LOCUS       DATAQL010000001         4251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5343598, whole genome shotgun sequence.
ACCESSION   DATAQL010000001 DATAQL010000000
VERSION     DATAQL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5343598"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VHS97_00005"
     CDS             complement(<1..1070)
                     /locus_tag="VHS97_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX3446605.1"
                     /translation="MSARAVHLVIDARPRGPHGLLAVEVVLGKSVLGRLFDLAIENVP
                     ANEPIVVHARGEEHDRLRALDMGASHPDVVFVNGPPRADATVLRTDRLYDPARLKRSL
                     RRGLSPELAVVWRLDRLESLTTAEQELSRRLTYQPLGKYWAFPLAQRLADRLCPTSVR
                     PNALTIASGGLMIGAATLVGAGIHDWLGRSVVALSMAVALVLDTADGRLARLQGTSSA
                     FGQWLDQVLDEVADLALHAAIAWAAFCSNGSPIWLVVGIIYASGKYLFLVQSLLGNEL
                     EEGQKKPPHIGLLPGVGTGSAQGRRGIDRIAGMVRLLGHADLRWHLWIVLALVGRLDV
                     ALAAYAGYFVLRSLAGVIRKGVR"
     gene            complement(1169..2125)
                     /locus_tag="VHS97_00010"
     CDS             complement(1169..2125)
                     /locus_tag="VHS97_00010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3446606.1"
                     /translation="MEPLTIDRTHILVLNYNGRGLLADCLPSIVAAARSSPVRCRVTV
                     VDNGSDDGSRELVATDWPNVDFVSETNLGLASFNRVLERLEEPVVLLLNNDIKLDSGA
                     VGPLIKVFETHGDALFSAPLCWSFDGRTYEGMRTRVRTRFGLVQGMCRVPGFEKFLNQ
                     PDLTAASGPVLAVDRRKFLALGGYDPIYFPGRIEDLDLGFRAWMAGCRGYFVPESIAY
                     HLGFGTFQPQLGKGRCDQLASRNTLIFMWKNTGGIRLLRHFAWLPIRFAFALLRGRLS
                     FASGLLAALSRVHRVLAARRILAVGQGDWVERQEKFFERFEW"
     gene            complement(2956..3357)
                     /locus_tag="VHS97_00015"
     CDS             complement(2956..3357)
                     /locus_tag="VHS97_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3446607.1"
                     /translation="MSQTTQERNKALVLDAFNTLFNKRDYEAAQRYWSPDYIQHSAHI
                     APGREGLFNLVRSMPPTLKYQPGMIVADGDIVIVHGRFSGFGAPVNWIAADILRIEDG
                     VFVEHWDVIQDEATQEQSKSGRPMFGDSFPL"
     gene            complement(3409..>4251)
                     /locus_tag="VHS97_00020"
     CDS             complement(3409..>4251)
                     /locus_tag="VHS97_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX3446608.1"
                     /translation="FNDHLSDLDKVVERILPFDVVCVMRERTPLPRSVIERLPRLKLI
                     ASTGTRNAAIDIDAAAERGIVVAHTGYNARPTIEMTWALILASARQVASENAHLRAGG
                     WQLTVGDSLHGKTLGVLGLGNIGSEVARIGLAFGMEVIAWSQNLTADKAQTCGARLVS
                     KEELFRGADILTIHLVLSQRTKGLVGAAELQSMKPSARLINTSRGPIVDEPALIEVLR
                     DRRIAGAALDVFDLEPLPADHPFRSLDNVLATPHIGFVARELYRTFYRDTVENITRWL
                     DHRT"
BASE COUNT          873 a         1336 c         1259 g          783 t
ORIGIN      
        1 cgaaccccct tcctgatcac gccggccaaa gaccgcaaca cgaagtaacc ggcataggcg
       61 gccagtgcga cgtcgagccg gcccacaagt gccagtacaa tccacagatg ccaccgcagg
      121 tcggcatggc cgagtagtcg aaccattcct gcaatccggt cgatcccccg gcgcccttgg
      181 gccgatccag taccgacgcc aggcaataag ccaatatgcg gtggcttttt ctgtccctcc
      241 tccagctcat ttcccaggag cgactggacg agaaagagat acttacccga ggcataaatg
      301 atcccgacaa cgagccaaat tggtgagccg ttgctgcaaa atgctgccca ggcgatagcg
      361 gcgtgcaagg ccaggtcggc gacttcgtcc aagacctggt cgagccactg gccgaaggct
      421 gaactggttc cctgcaaccg ggcaaggcgg ccgtcggcgg tgtccaggac gagcgccaca
      481 gccattgata gagcgacgac cgaacggccg agccaatcgt gaatgccggc gccgaccagc
      541 gtcgccgccc cgatcatcaa acctccggag gcaatcgtca gggcattggg gcggaccgac
      601 gtcgggcaga gccgatccgc cagacgctgg gccaggggaa acgcccagta tttgcccagc
      661 ggctgatagg tcaatcgacg cgacaactct tgctcggcgg tcgtcagcga ctccagccga
      721 tcaagccgcc atacgacggc cagctccggc gaaagccccc gccgtaggct gcgcttgaga
      781 cgggcaggat cgtagaggcg atcggtccgc aaaacggtgg catccgcgcg aggcggccca
      841 ttgacgaaca cgacgtcggg gtgactagcg cccatatcaa gcgcgcggag ccggtcatgt
      901 tcctcgccgc gcgcgtgaac gacgatcggc tcgttcgccg ggacgttctc gattgccaga
      961 tcgaacagac ggcccagcac gctcttgccc agaaccacct cgaccgccag caacccgtga
     1021 ggaccacgtg gtcgggcgtc gatcacgaga tggacggccc gagcagacat caatcgcctc
     1081 cgtactagtt ccaaacaact atcttttcga gcatccggcc gacagagccc gcgtcacggg
     1141 tcttctaaag ttcgcaccgt cccgtgacct accactcaaa ccgctcaaag aacttttctt
     1201 gcctctcgac ccaatccccc tgccccacgg cgagaattcg cctcgccgcg agaacgcgat
     1261 gcacgcggga caaggctgcc aataaccctg atgcgaagct caatcttccg cgcaagagtg
     1321 caaacgcgaa ccggatggga agccacgcga aatggcgcaa gagacggatt cccccggtgt
     1381 ttttccacat gaagatcaag gtgttccggc tggccaactg atcgcaccgc cccttcccta
     1441 actgcggctg gaaagtgccg aagcccaggt gataagcgat tgattccggt acgaaatagc
     1501 cgcgacagcc ggccatccag gcgcggaacc ccagatcgag atcttcgatc cgcccgggaa
     1561 agtagatcgg atcgtaaccc ccgagcgcga ggaatttgcg acgatcgacg gccagcactg
     1621 ggccggacgc ggcggtcagg tctggctggt tgagaaactt ttcaaagcct ggcacgcggc
     1681 acatcccctg cacgagccca aatcgggtcc gtactcgcgt cctcatccct tcgtaagtgc
     1741 ggccgtcgaa cgaccagcac aaaggcgccg agaacagggc gtcaccatgg gtttcaaaga
     1801 ccttgattag cggcccgacc gcgccggaat cgagcttgat gtcattgtta aggagcagaa
     1861 cgacaggctc ttcgagccgc tcgagcaccc gattgaatga cgcgagcccg aggttcgtct
     1921 cactgacgaa gtcgacgttc ggccagtccg tcgcgacgag ttcgcgcgat ccatcgtcgg
     1981 aaccattgtc gacaaccgtg acacgacacc ggactggtga cgaacgagcc gcggccacaa
     2041 tcgatggcaa acaatcggcc agcaagccgc gaccgttata gttgagtacg aggatatgag
     2101 tacgatcgat cgtcaagggt tccatccctc gcgctaagct gtgcatcctg cacatcgaat
     2161 tgatcaatcg acgaaataga acaaagtcgc cggcttcgag acccgttttc atacgttcgc
     2221 gtgggtgcat accataagtc gggagtcgat tgtggtcaac cccgtcacgg aatggtgcgt
     2281 agggtggtgc cgagccgcga gaaatcgcgg cgagccccac cgacccgccg tggtgcatgg
     2341 cgggcctccc gatcccgagg ttcgttcggc aatggtgcat agtgggcctc ccgattgctg
     2401 ggatcgttcg gctatagtgc atagtgggcc tcccgatccc gcggatcgtt cggctatagt
     2461 gcatggtggg cctcccgatc cctgggatcg ttcggctata gtgaatggtg ggcctcccga
     2521 tccctgggat cgttcggcga tagtgcatgg cgggcctccc gatcccgagg atcgttcggc
     2581 accaccctac tcccgctgcg ccgcggtcgc gttaggcact acgcacttgt tccctgaaca
     2641 gctcggatct ggcgcacaca ctcatcgtcc gcgaaattca ctcttcggaa ccaaagcatt
     2701 gccgcacagg cagttgcgca tgtcgctgga ataatcggca caggttagca agtcgtgatt
     2761 gcttgtccga attttagttg cgcatgtcgc tggaataatc gatattcgtg gcatgatcac
     2821 tactgccgcg ttgtaggccg tcccggcaaa ttcaaccggt attgtgtccc tatcgcctta
     2881 gacaacggga aatttcctca ggtcactcac ggcgcgcccg gtcgggaacc tgcgcgacgg
     2941 gcgtcccggt caccgctata acggaaacga gtcgccgaac atcggccggc cgctcttgga
     3001 ttgctcttga gtagcctcgt cctggatgac gtcccagtgt tcgacaaaga ccccatcctc
     3061 gatccggagg atgtccgccg cgatccaatt cacgggtgcc ccgaaacccg agaaacgccc
     3121 gtggacaatg acgatgtcgc cgtcagcgac gatcatcccc ggctggtatt tcaaagtcgg
     3181 tggcatgctc ctgacgaggt tgaagaggcc ctcgcggcct ggtgcgatgt gcgcgctgtg
     3241 ctgaatgtag tcgggcgacc agtacctctg ggcggcctca tagtcccgct tgttgaacag
     3301 tgtgttgaag gcatcaagga ccagtgcctt attcctctcc tgcgtcgtct gactcatcac
     3361 gatctcctgc ccgtttagcg actgcggtgc tggaatgtta cgcatcgttc aggtccgatg
     3421 gtccaaccat cgcgtgatgt tttcgaccgt atcgcggtag aaagtcctgt agagttctcg
     3481 agcgacgaaa ccgatatgcg gtgtagccag gacgttatcc agcgatcgga acgggtgatc
     3541 cgccggcaaa ggctcgaggt cgaacacgtc gagcgccgcg ccggcaatgc ggcggtcacg
     3601 caagacctcg atgagcgccg gctcgtcaac gatcgggcca cgcgaggtat tgatgagcct
     3661 ggccgatggc ttcatggact gcagctcggc cgctccgacc aggcccttcg tcctttggct
     3721 cagaacgagg tggatcgtca ggatgtcggc gccgcggaag agttcctcct tggagacgag
     3781 ccgcgctccg catgtttgcg ccttgtctgc cgtgaggttc tggctccagg cgatcacttc
     3841 catgccgaac gcgagcccga ttcgcgcaac ctccgagccg atgttgccca ggccgagaac
     3901 gccgagcgtc ttaccgtgga gactgtcgcc caccgtgagc tgccagccgc cggcgcgcag
     3961 atgtgcgttt tccgaagcga cctgacgcgc gctcgcaagg atgagagccc aggtcatctc
     4021 gatggtgggg cgtgcattgt atccagtatg cgctaccacg atacctcgct cggccgcggc
     4081 atcgatatcg atggccgcgt tgcgagtacc ggtcgatgcg atcagcttca gtcgcggcag
     4141 ccgctcgatg accgacctcg gcagcggtgt gcgctcgcgc atgacgcaga ccacgtcgaa
     4201 cggcaggatc cgctcgacaa ctttgtctaa atcggacaga tggtcattga a
//
LOCUS       DATAQL010000002         4818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3511648, whole genome shotgun sequence.
ACCESSION   DATAQL010000002 DATAQL010000000
VERSION     DATAQL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511648"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VHS97_00025"
     CDS             <1..1313
                     /locus_tag="VHS97_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3446609.1"
                     /translation="EVDIVLFPAQRLGDLVDAGALAKIPNVAVVPPKPPENEAGDRDQ
                     REQDQTDATFADVFRYMDIAPAFREQISRYGPDRLALPCGGSALVLVYRRDAFERAPN
                     KTAAQQAGLTLEPPTTWEQLDSLAKFCHGRDWNGDGKPDMGIALTLGAGSEGVGDATF
                     LARAASLGQHRDHYSFLFDSDTMIPRIDTPPFALALRGLVALKAYGPPGMERFDADSA
                     RAAFRTGDVALLIDRAERADTWSHGKPIGVAPLPGSDRVYEPIRKEWVPASPRNAPSY
                     LPRGGGWLIGLSAQLSGTQLEAALDFAKYLGGPENSPRIRGERTFPMLPVRTSQMGQG
                     LPDPMSAPDVDSRLWSDAVSRTLLAERVVPGLRIPQADGFLADLAKGRAAAAGGATAE
                     EALANVAKAWTERTKGYGPKRLLWHYRRSLNSLATLPEPPDLGK"
     gene            1326..1907
                     /locus_tag="VHS97_00030"
     CDS             1326..1907
                     /locus_tag="VHS97_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446610.1"
                     /translation="MDDTEGSPPTLNADIETEVKELMGLFDLPAFARRGQDLEIILRR
                     LHERCRRTRSEMLDMVHLRLRQWSRAVNGPTAWSGVFIRSIEPLWPLSGAEEGAWADS
                     LAPIRRRRLIASDLKSAVVRFNRRWTQFLERLNLEPANAAITQYNRYYVLEKECVMGS
                     PRLAARHFIPVPQLTTEMLANDHPTLPVPELIE"
     gene            1969..3555
                     /locus_tag="VHS97_00035"
     CDS             1969..3555
                     /locus_tag="VHS97_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3446611.1"
                     /translation="MKVPFQLIGRTFLAGLAVMSAGASLAGPAAPQRAADNSNEPNKP
                     AIRAGVLAFEVTCAASLRNEPIKGRVYVMLASAGSGVEPRLGPNWFRPQPFFAKDVEN
                     WRPGEPLQIDSGADGFPSPLEEIEPGKYAIQAVARLNLDTHKIGDGEGNLYGPVVHTE
                     LDPKRGCTVALQIDHAVGPRAFKATERIKLVELPSPMLSAFHHRPIKHRAAVILPKES
                     AREWQAAKLPTVYIIPGFGGDHYMATAMADSPRMAYGNDFIRVVLDPDCGTGHHVFAD
                     SATNGPRGTALVEEFIPHIESSFPVLANPRARLLNGHSSGGWSSLWLQVAYPDFFGGT
                     WSTSPDPVDFRDFQRINLYASGENMFRDRDGKRRPIARMGGAPALFYDRFCKMDDVIG
                     WGGQLGSFEAVFSPLDQKGRPRKLWDRSSGAIDPEVAKAWEPYDIRLVLERNWPELGP
                     KLKGKLHVIMGDVDTFYLEGAVKLLKESLAQLGSDAVVDVIAGRDHGNLLDAPLAGRI
                     NREMHAAVQSVLAAQPPARD"
     gene            3599..4471
                     /locus_tag="VHS97_00040"
     CDS             3599..4471
                     /locus_tag="VHS97_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446612.1"
                     /translation="MPVLEREEYIEQAYFFHTFRERLIDGLPSQEILARIGEELLSTT
                     KLPLAVSFLHVEMKGTGQMGPAMERISHYFTPFQAHVIGQSENDLSRFAMDQALLVLE
                     REAKFKADGPSLPALFVFQFEALSRNRLGYIKGLAAMASDPFYTEDWTDYIMTLRTRL
                     GDVDFADLIFVRSEQFVHERQRLNPEFVPKFPILFGEKEGKIARANRGRDPMYLFAAL
                     QRQLGYPEVPRPRRPDELEGRVLLLEQKIALLENRLKSAESDLQQDVDLAQVVVKPED
                     TAGIPQGWGQKGAK"
     gene            4551..>4818
                     /locus_tag="VHS97_00045"
     CDS             4551..>4818
                     /locus_tag="VHS97_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446613.1"
                     /translation="MKASNRVFWVGLLLFFSAHCPLRTAYSQEFDRIVVDANFPGAYQ
                     VEVADVNGDSKPDIVAVGGSTCAWYENPTWKKRVVTGSQETPGII"
BASE COUNT          919 a         1514 c         1479 g          906 t
ORIGIN      
        1 gtgaagtcga catcgtgctc ttccctgccc aacggctggg cgatcttgta gacgccggcg
       61 cactggcgaa gatacccaac gtggctgtcg tccccccgaa gccgccagaa aacgaagcgg
      121 gcgaccgcga ccagcgagag caagatcaga ccgatgccac gttcgctgac gtctttcgat
      181 acatggacat cgcaccagcc tttcgcgagc aaattagccg gtacggtcct gatcggctcg
      241 cgctaccgtg cggaggctcg gcccttgtgc tcgtttatcg ccgcgatgcg ttcgagcgtg
      301 cgcctaacaa aacggcggca caacaggcag gcttgaccct ggagccgccg acgacctggg
      361 aacagcttga ttccctcgca aaattctgcc acggccgcga ctggaacggc gacggtaaac
      421 ccgacatggg aattgccctg acgctcgggg ctggcagcga aggtgtaggc gacgctacgt
      481 tcctggctcg ggccgcgagc ttgggacagc atcgggacca ttactcgttt ctcttcgatt
      541 ccgacacgat gattccgcga atcgatacgc ctccgtttgc cctagcgctt cggggtttgg
      601 tcgcactgaa ggcatatggc ccacccggta tggagcggtt cgatgccgac tcagcccgcg
      661 cggcgtttcg cacgggcgac gtcgcactgc tgatcgatcg tgccgaacgc gccgacacct
      721 ggtcacacgg caaaccgatc ggggtcgccc cactgccggg ctcggatcgc gtctatgagc
      781 cgatccggaa agaatgggtc ccggcttcgc cgcggaacgc acccagctac ttgccccgag
      841 ggggcggctg gttgatcggt ttgagcgccc agttgagcgg cactcaactc gaggcggcgc
      901 ttgacttcgc caagtaccta ggcggcccgg aaaactcgcc gcgcattcga ggcgagcgga
      961 cctttccgat gctgcctgtt cgcactagcc agatggggca aggtttgccg gacccgatgt
     1021 cggccccgga tgtcgactct cggctctggt cggacgcggt gagcaggact ttgctggccg
     1081 aacgagtcgt gccgggtttg cgcatccccc aggctgacgg cttcctggcc gacctggcca
     1141 agggccgcgc ggccgcggcc ggcggcgcga ctgcggaaga ggctcttgca aatgtagcca
     1201 aggcatggac cgagcgcacc aaagggtacg ggcccaaacg cttgctatgg cattatcggc
     1261 gcagtctcaa cagcctggcg acgctcccgg agcctccgga tctcggaaaa taagagggcg
     1321 caaccgtgga tgacaccgaa gggtcgccgc cgaccttgaa cgccgacatc gagacggaag
     1381 tcaaggaact gatgggcctc tttgacttgc cagccttcgc gcggcgcggg caggacctcg
     1441 aaatcattct caggcgctta cacgagcgct gccggcgaac gcgcagtgaa atgcttgaca
     1501 tggttcactt gcggctccga cagtggtcgc gcgcggtaaa cggtccaact gcctggtccg
     1561 gtgtcttcat ccgctccatc gagcccctgt ggcccctctc gggagccgag gaaggagcgt
     1621 gggccgactc actcgctccc attcgccgcc ggaggttgat cgcgtccgac ttgaaaagcg
     1681 cggtagttcg gttcaaccgg cgctggacgc aattcctgga gcgtttgaac ctcgagccgg
     1741 ccaacgccgc catcacccag tacaatcgtt attatgtcct cgagaaggag tgcgtgatgg
     1801 gttcgccgcg gctcgccgcc cgccacttta ttccggttcc ccagctcaca accgagatgc
     1861 ttgcgaacga tcatcccaca ctgccggtcc ccgagctgat cgagtgattc gcctcgaagg
     1921 caaagttcac tcactcaatt ccggcggccc taccacagat tggaaatcat gaaagtgcct
     1981 tttcagctta tcggacgaac ttttctcgcc ggcttggcgg tgatgtcagc gggtgcgagc
     2041 ctggccggcc cggcggcccc acaaagagcg gcggacaatt cgaatgagcc aaacaaaccg
     2101 gcaatccgag ccggggttct ggcgttcgag gtcacctgtg ccgccagttt gcgaaacgag
     2161 ccgatcaagg ggcgggttta cgtcatgctc gcgtcagccg gttcgggggt cgagcctcgt
     2221 ctcggcccca actggttccg accccagccg tttttcgcga aggacgttga gaactggcga
     2281 ccgggtgagc cgttacagat cgactctggc gcggacggct tcccgagtcc gctcgaggag
     2341 atcgagccgg gcaagtacgc catccaggcg gtcgctcggc tcaacctcga cacgcacaag
     2401 atcggcgacg gcgaggggaa cctctacgga cccgtcgtgc ataccgagct cgatccgaag
     2461 cgcggatgca cggtcgcgct tcagatcgat cacgccgtcg ggccccgggc gttcaaggcg
     2521 accgagcgaa tcaagctggt cgagctgccg agcccgatgc tgtcggcttt tcatcaccgg
     2581 ccgatcaagc atcgcgcggc ggttatcttg cccaaagaga gcgctcgaga gtggcaggcg
     2641 gccaagctgc cgacggttta catcatcccc ggcttcggcg gcgaccacta catggcaacg
     2701 gccatggccg acagccctcg gatggcttat ggcaacgatt tcattcgcgt ggtgctcgat
     2761 cccgattgcg gcaccggcca tcacgtcttc gccgacagcg cgaccaacgg accccgcggc
     2821 acggccttgg tggaggagtt cattccccat attgagagct cattccctgt actggcgaat
     2881 ccgcgcgccc gcttgcttaa tggccattca tcgggaggct ggagcagcct ttggcttcag
     2941 gtggcctacc ccgacttctt tggcggtacc tggtcgacca gtcccgaccc agtcgacttt
     3001 cgcgattttc aacggatcaa tctttacgcg tccggcgaaa atatgttccg cgaccgcgac
     3061 ggtaaacggc ggccgatcgc caggatggga ggcgcgccgg cgctttttta cgaccggttt
     3121 tgcaagatgg acgacgtgat cggctggggg ggccagcttg gctcgttcga ggccgtcttt
     3181 agcccgctcg accagaaggg ccgcccgcgc aagctctggg accgatccag cggcgcgatc
     3241 gatcccgagg tcgccaaggc gtgggagccc tacgacatcc ggctcgtcct cgagcggaac
     3301 tggccggagc tcggccccaa gctcaagggc aagctccacg tcatcatggg cgacgtggat
     3361 acgttttacc ttgaaggggc tgtcaagctc ctgaaagagt ctctggccca actggggagt
     3421 gatgcggtgg tcgacgtgat cgccggccgc gaccacggca acctgctcga cgcgccgctc
     3481 gcagggcgga tcaaccgcga gatgcacgcg gccgtccaat ccgtgctcgc cgcacaaccg
     3541 cccgcgcgcg attagtccgt ttcaggatga acaagggcaa acaccgagga gtatgccgat
     3601 gcccgtgctc gagcgtgaag agtatatcga gcaggcgtac ttcttccata catttcgcga
     3661 gcgactgatc gacggcctgc cctcgcagga gatcctggca cgaatcggcg aagagctgct
     3721 gtcgaccacc aagctgccgc tggctgtctc atttctccat gtcgagatga aggggactgg
     3781 ccagatgggg ccggccatgg aacggatcag ccactatttc acgccgtttc aggcgcacgt
     3841 tattggccag tctgaaaacg atttgagccg tttcgccatg gatcaggccc tcctcgtgct
     3901 ggaacgtgaa gccaaattca aagccgacgg cccgtcgctt cccgctctct tcgtttttca
     3961 attcgaggcg ctgtcgcgca accgcctggg atacatcaaa ggactggccg ccatggcgtc
     4021 cgaccccttc tataccgagg attggaccga ttacatcatg acgctgcgca cgcggctggg
     4081 agacgtcgat tttgccgacc tcatattcgt tcgttccgaa caatttgtcc atgaacgcca
     4141 gcgcctgaat cccgaatttg ttcccaagtt tccgatcctt ttcggcgaga aggaaggaaa
     4201 gattgcgcga gccaaccggg ggcgcgaccc gatgtacctg ttcgcggcgt tacagcgcca
     4261 gctcggctac cccgaggtcc cccggccccg taggcccgac gagctcgagg gtcgcgtcct
     4321 gctcctcgag caaaagattg cactgctcga aaatcggctc aagtccgcgg aaagcgacct
     4381 ccagcaggac gtagatctcg cgcaggtggt ggtcaagcct gaagataccg caggcatacc
     4441 gcaagggtgg ggtcaaaagg gggcgaaata gtcattggtc attggtcctt ggtccttgga
     4501 taatactcgg agactaagtc agagtttgat ccgaacccct gaggtgtccg atgaaagcgt
     4561 cgaaccgtgt gttttgggtt ggcctccttc ttttcttcag tgcccactgc ccactgcgca
     4621 ctgcgtactc tcaagaattc gaccgcattg tcgtcgacgc aaacttcccg ggcgcctatc
     4681 aggtcgaggt ggctgacgtc aacggcgaca gcaagcccga catcgtggct gtgggcggca
     4741 gcacgtgcgc ctggtacgaa aatccaacat ggaaaaagcg ggtcgtcacg ggttcccagg
     4801 agacgcctgg aatcatct
//
LOCUS       DATAQL010000003         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1374279, whole genome shotgun sequence.
ACCESSION   DATAQL010000003 DATAQL010000000
VERSION     DATAQL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374279"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="VHS97_00050"
     CDS             complement(<1..1611)
                     /locus_tag="VHS97_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3446614.1"
                     /translation="MTFASLLLALLTATNSPASDASDGPALLDFHAEWCGPCHKARPA
                     VAQLIRQGYPIKTIDIDQEPKLRARYHVDQVPTFIVVDGAGRELDRTTGPQPAADLAK
                     FYKAAIAKVQPPASSNAHVGSREDSRGDADDREDPPASSRESSGNDDHDRPESEGDAA
                     EPPFTNPKPWESVVRIRVLGNHSTGFGSGTIIHSTPQESIILTCAHIFKIEGRKQVQP
                     SEFQRKIMIDLFDGTLRGPKKNMVHFVEQVEGRAVDYDFNRDVGLIRIRPGRRLPACR
                     IVPDYWQPKSRMAVLTVGCSEGHDATAWNTVISRPRIQNFLSGNPTYEAIECNEAPKQ
                     GRSGGGLFTEDGYVAGVCNFAEPQGNHGLYATPRSIYHILDRNNMMAFYSPVSRGSGT
                     VVADGRAGSQPRRNVPVAVERGQSPDHEEAPRNRTDGEIVKVPSPNFLGIVDPVAESA
                     ERTARAASGTTTRRTAWQPHPVPGAPASVKTKAEPTDLNLDPSADHDHFGPPSSDPQT
                     QEGTGDQADSKPASLQGATSGSRWRPVKA"
BASE COUNT          368 a          648 c          731 g          459 t
ORIGIN      
        1 tgccttgacg ggccgccatc gggagcccga agtcgcgccc tgcagtgacg cgggcttcga
       61 gtctgcctgg tcgcctgtgc cttcttgtgt ttgcgggtcg ctggagggcg gaccaaaatg
      121 gtcatgatcg gccgacgggt ccaagtttaa gtcggtcggc tcagcttttg ttttgaccga
      181 ggccggcgcg cccggaacgg gatgtggctg ccaggctgtg cgcctggtgg tggtgcccga
      241 ggctgcccgg gcggtccgtt ctgcagactc cgccaccggg tcaacgatgc ccaaaaaatt
      301 gggggacggg accttcacga tttcgccgtc ggtgcggttt cttggagcct cttcgtggtc
      361 gggcgattga ccgcgttcga ctgcgactgg gacgtttcgc cgtggttggc tacccgcccg
      421 accatcggcc acgaccgtgc ccgagccgcg cgagaccggc gaataaaatg ccatcatatt
      481 gttgcggtcc agaatgtgat aaatggaacg aggcgtcgcg tacaagccgt gatttccttg
      541 cggttcggcg aaattgcaga cgccagcgac gtagccgtct tccgtgaaca gtccaccgcc
      601 ggagcgcccc tgtttgggtg cctcgttgca ttcgatggct tcgtaggttg gatttcccga
      661 caagaagttc tgaatccgcg gtcggctgat gacggtattc caggcggtcg cgtcgtgtcc
      721 ttccgagcag ccgaccgtga ggaccgccat gcgcgatttg ggttgccagt agtcggggac
      781 gattcggcaa gccggcagac ggcggccggg cctgatccgg atcagaccga catcacgatt
      841 gaaatcgtaa tcgacagcgc gcccttcgac ctgctcgaca aaatggacca tgttcttttt
      901 gggtccccga agtgtgccgt cgaacaggtc aatcatgatc ttgcgctgaa attcagacgg
      961 ctggacctgc ttgcgcccct cgattttgaa tatatgcgca caagtgagga tgatcgactc
     1021 ctgaggcgtg ctatggatga tcgtgcccga gccaaaacca gtggaatggt tgccgagaac
     1081 ccgaatccga accacggatt cccatggctt gggattggta aacggaggct cggcggcgtc
     1141 gccctcgctc tcggggcggt cgtggtcgtc gtttccactg gattctctcg acgaagcggg
     1201 cgggtcctcc ctgtcgtcgg catctccgcg cgagtcttcg cgcgagccta catgggcatt
     1261 ggagctcgct ggcggctgta ccttggcgat ggcggccttg tagaactttg caaggtcggc
     1321 ggccggttgt gggccggtcg ttcggtcgag ctcgcgtccc gctccgtcaa cgactatgaa
     1381 cgttgggacc tggtcgacat gatagcgcgc gcggagcttg ggctcctggt cgatgtcgat
     1441 cgtcttaatc gggtacccct ggcgaatcaa ttgcgcgacg gcagggcggg ccttgtggca
     1501 cggtccacac cactcggcat gaaagtcgag cagggccggg ccgtccgagg catcgctcgc
     1561 ggggctgttc gttgcggtca agagcgcgag caacaaggag gcgaaggtca tgccgccagg
     1621 actcctttcc ctcgggatag gccgcgagcc aacgtcggag tgacgtcgcc gccgggctgt
     1681 accagtctgg tgttacgcgc ttcggcagga agcgaggaac atatctctgg ttgggcctgt
     1741 tgccaggatc gggcgctctg gttcgtcgac tccttcgcgg accgggtacg cgccccgccg
     1801 aaaaggattg gacggaccac gtccgcttga gcggacgttt tcgccgaaag aagcagatgt
     1861 gatacgagat gatgggcgat ttgccgggga ggaaatcggt ccccgaccct tccttgggcg
     1921 gcgcgcctct gcctgagggc cccgcgacac accatcttgg cgtgccgcgt cccgccgttt
     1981 gagccatcta gccgcacaaa cgtctcgtcg ccgatgcggg cgagcacgtc cagttggcga
     2041 aggtccaagg gcggtcccga ggttgccgcg agctccttcc tggtatctaa cccccctcga
     2101 catccggtcg agggtgtgcg agatcccgag gggttcgcgt tcaccgcggt gagccccttt
     2161 tactaggagt ggacgaggag taccagatca cttatcgact tttcga
//
LOCUS       DATAQL010000004         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2901011, whole genome shotgun sequence.
ACCESSION   DATAQL010000004 DATAQL010000000
VERSION     DATAQL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901011"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VHS97_00055"
     CDS             <1..1067
                     /locus_tag="VHS97_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3446615.1"
                     /translation="QKVKSLGLWAPQIPRSLGGLGLNCTEYALVGEELGKSPLGHYVV
                     NGQAPDAGNMEILREFGTDAQQERWLKPLVRGEVRSCFAMTEPEHAGSNPVWMSTTAV
                     RDGEDYVLNGRKWFASAADGASFAIVMAVTDAHAEAYRRASLIVVPTATVGFQRVRNI
                     PCMGHVGDDWASHAELVFDQCRVPRENLLGEEGTGFAMAQARLGPGRIQHTMRWIGIC
                     RRALEMMCSRAATRQLAPGDPLGSRQTVQNWIAESRAAIDAARLLVLHAAWTIDTQGI
                     AAAREAISLIKFHVAGVMQEVVDRAIQTHGALGISDDTVLSWFYRQERAARIYDGPDE
                     VHKVVVAKRLLKQHGMSNSS"
     gene            1101..2171
                     /locus_tag="VHS97_00060"
     CDS             1101..2171
                     /locus_tag="VHS97_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX3446616.1"
                     /translation="MSAQIDETRPVRDGEQLDTARLRDYLAAHLPGAEGLLEVEQFPH
                     GHSNLTYLLRMGGQEWVLRRPPFGNRVKTAHDMGREYRILSRLCHVYAPAPAPVLYCD
                     DESVVGAPFYLMERRKGVILRGAPPADRPMPPELVTRLCETLVDGMALLHGLDYRAAG
                     LEDVGKPQGYVERQVTGWTKRYHDARTEDVPDLERTATWLAENQPAESGGTTLIHNDF
                     KFDNLVLDPGDLTRIVAVLDWEMATIGDPLMDLGTTLAYWTEPSDPAPLHRFIVGPTT
                     QPGSLSRQGLVERYGRTAGRDISHMLFYYVCGLFKVAVIAQQIYARFVRGLTHDPRFA
                     GFNHVVAALGLGAVRAIDSKRI"
     gene            2196..2960
                     /locus_tag="VHS97_00065"
     CDS             2196..2960
                     /locus_tag="VHS97_00065"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3446617.1"
                     /translation="MAQTIDLTGKVALVTGGGRGIGKAIAGRLADAGASVVIASRKRE
                     NLEAAAADLSRLSGKVVPIVCHLGKLDQIEALMHQTESQLGPVDILVNNSATNVGQGP
                     ALDVTDLMLDKMIEINIKAALRLIRLTVPTMIERGKGGSIINVSSISGLQPQTGGLLY
                     SFTKAGLNMMTRSWAREFGKHGIRVNALAPGLIQTDFSAYFWEDEDRRREFVSEQPIG
                     RLGQPDDVAGLALYLASDQASFVTGQVFVVDGGLTA"
BASE COUNT          546 a          990 c          960 g          510 t
ORIGIN      
        1 gccaaaaggt caagtcgctg ggactctggg cgccgcagat cccccggtcc ttgggcgggc
       61 tcgggctcaa ttgcacggaa tatgccctgg ttggagaaga gctgggcaag agcccgctcg
      121 gtcactacgt cgtcaacggc caggcacccg acgccggcaa catggaaatc ctccgcgagt
      181 tcggcaccga cgcccagcaa gagcgctggc tgaaaccact cgtgcgcggc gaggttcgta
      241 gttgctttgc catgaccgag cccgagcacg ccggttccaa cccggtctgg atgagcacaa
      301 cggcggtccg cgatggagaa gattacgtac tgaacggccg caaatggttt gcctcggcgg
      361 ccgacggtgc gagcttcgcc atcgtgatgg ccgtcaccga cgcccatgcc gaggcctacc
      421 gccgcgccag cctgatcgtc gttccgaccg ccacggtcgg cttccagcgc gtgcgcaata
      481 ttccgtgcat gggacacgtc ggcgacgatt gggccagcca cgccgagctg gttttcgatc
      541 aatgtcgggt cccgcgcgag aatttgcttg gcgaagaggg aaccggcttc gcgatggctc
      601 aggcgcgcct gggcccgggg cggatccagc acacgatgcg ctggatcggc atatgccgcc
      661 gcgctctcga gatgatgtgc agccgggccg ccactcgtca gctcgcaccc ggcgacccgc
      721 tcggaagccg gcagactgtg caaaactgga tcgccgagtc gcgcgcggcg atcgacgcgg
      781 cacggctgct tgtcttgcat gcagcctgga ccatcgacac ccaggggatc gctgcggccc
      841 gcgaggcgat ctcgctcatc aagtttcatg tggcgggcgt catgcaggaa gtcgtcgatc
      901 gggccatcca gacccacggc gccctgggga tcagcgacga caccgtcctt tcctggttct
      961 accggcagga gcgcgcggcc cggatctacg atgggcctga tgaggtccac aaagttgtcg
     1021 tcgccaaacg cctcctgaaa cagcacggca tgagcaactc gtcttgaatt ccccgcactc
     1081 gatccctcgc gaggccggcg atgagcgctc aaatcgatga aacacgcccc gtgcgcgacg
     1141 gcgaacagct cgacaccgcg cgattgcgcg actacttggc cgcccatttg ccaggagccg
     1201 aaggactgct cgaagtcgag cagttccctc acgggcactc caacctgact tacttgctcc
     1261 gcatgggagg tcaggagtgg gttttgcgca ggcctccctt tggcaatcgg gtcaagacgg
     1321 cccatgacat ggggcgcgag taccgcatcc tctcccggct ctgccatgtc tacgcgcccg
     1381 cgccggcgcc tgtactgtac tgcgacgacg agagcgtcgt cggagcaccc ttttacctga
     1441 tggagcggcg caagggcgtc atcctccgcg gcgcgcctcc cgccgaccgc ccgatgccac
     1501 cggagctcgt gacacggctc tgtgagacgc tcgtcgacgg catggcgctc ttgcacggac
     1561 tcgactatcg cgccgccgga ctggaagacg tggggaagcc tcagggctat gttgagcgcc
     1621 aggtgacagg ttggaccaag cgctaccacg acgcacgcac cgaggacgtc ccggatctgg
     1681 aacgaaccgc aacatggctc gccgagaacc agccggccga gtcgggtggg acgaccttga
     1741 tccataacga tttcaagttt gacaatctcg tgctcgaccc cggcgacctc acacggatcg
     1801 tggccgttct cgactgggaa atggccacca ttggcgaccc gctcatggat ctgggtacga
     1861 cgcttgccta ctggaccgag cccagcgacc ccgcgccctt acaccggttc atcgtcggcc
     1921 cgacgaccca gcccggcagc ctgagccgcc aggggctggt cgaacgctac ggtcggaccg
     1981 ccggacgcga catctcgcac atgctcttct actacgtgtg cgggctgttc aaggtcgccg
     2041 tcatcgccca gcagatctac gcccgatttg tccggggcct gacccatgac ccccggttcg
     2101 ccggtttcaa tcacgtcgtg gccgcgctcg gactgggcgc ggtcagggcg attgattcga
     2161 agcggatttg acgatctcaa cggagacgac gcacgatggc acaaacgatc gatttgacgg
     2221 gtaaggtcgc cctggttacc ggcggcgggc gcggaattgg caaggcgatc gccggccggc
     2281 tggccgacgc cggcgcgtcc gttgtgatcg ccagccgcaa gcgagagaac ctcgaggccg
     2341 cggccgccga cctcagccgt ttgagcggca aggtcgtccc catcgtttgc cacctcggca
     2401 agctcgacca aatcgaggcc ttgatgcacc agaccgagtc gcagctcggg ccggtcgaca
     2461 ttttggtcaa caacagcgcc accaacgtgg ggcaggggcc cgcgctcgac gtcaccgacc
     2521 tgatgctcga caagatgatc gagatcaata tcaaggctgc gctccgactg atccggctga
     2581 cggtgcccac gatgatcgag cgcggcaaag ggggctcgat catcaacgtc agttcgatct
     2641 cgggtctgca gccgcagacc ggcggcctgc tttacagctt cacgaaggcg ggtctgaata
     2701 tgatgactcg gtcgtgggca cgcgagttcg gcaagcacgg catcagggtc aacgcgctgg
     2761 cacccggctt gatccaaacc gactttagcg cctacttttg ggaagacgag gatcggcggc
     2821 gcgagtttgt gagcgagcag ccgattggcc gcctgggcca gcccgatgac gtcgcaggac
     2881 tggcgctcta tctcgcctcc gaccaagcct cgttcgtcac cgggcaggta ttcgtggtcg
     2941 acggcggact gacagcctga cggcgcgtac gaaccaggcg ttcgctggcg ccgtgcattg
     3001 atcggt
//
LOCUS       DATAQL010000005         5284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2748398, whole genome shotgun sequence.
ACCESSION   DATAQL010000005 DATAQL010000000
VERSION     DATAQL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2748398"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1788
                     /locus_tag="VHS97_00070"
     CDS             43..1788
                     /locus_tag="VHS97_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446618.1"
                     /translation="MSGTAGNEPSTALIHEGWNHLTSQRPLAAWGSWQRVLRANPDSQ
                     AAQQALATLESAADLPLAARTSYRFRQPSDPTRRTAWDARMRDQGDNELEATADVFGR
                     LAAEDPTDSAAWYNRALCLAWMGKNVEAIGCLDRVVGLEAERAFDLAVDAWTLAEVLR
                     QGGGAETLADDLRFACTVAWKPRDTSWLLEEFPEIQRIPTPRAPGAASDERSEIEVYE
                     WLDRPVSELTGRSPAPALPPTVLASVYVKGHTLRLSSPRVETLERIEEVLFPRLEIGG
                     GSVRREASPLPLPFLDADVWIFRIPPDLEPDHANHLSRESVERYLEDQWIHRPRHGLD
                     SRSPLAASVAASRGDTVARAKLTAVIRLREQLGNRPSAILLYQGYPFDRLRRRLGLEM
                     VYPTAVDVEDLGCASAGELDRLDPATLDDTRLVEAFMSAAGLKDDARTGRLATELLKR
                     RPIALKSLDLALVVAPLVRQSIARNHIDGAVSWLKEARSIGDLATATTLEIWIAEVYA
                     RADRPDRALKTYMRLIKPDAAGAALALDAGEMMLDNGHLDEAKVLLLLAGDMARRNGR
                     QWIERRARRLLDRLS"
     gene            2206..2520
                     /locus_tag="VHS97_00075"
     CDS             2206..2520
                     /locus_tag="VHS97_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446619.1"
                     /translation="MKIVPLEGTTMTVAELVELAQGESVILTREGNPLVSVKGITGSD
                     WESASLAHNPQFAELIERSRRSYREQGGIGMDQMRRELGLESHPSDVSTAHACNEAET
                     GD"
     gene            2754..4514
                     /locus_tag="VHS97_00080"
     CDS             2754..4514
                     /locus_tag="VHS97_00080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3446620.1"
                     /translation="MPRVRTIMTLRAHVAATCLLFLVGPNGRTALANADADVILHHGK
                     IVTADRAFSIHEALAVKGDQVIQVGSDADVLRSRGPNTTLVDLAGKMVLPGLIDSHTH
                     PTDACMIEFDHPLPEMETIADVLKYIHARAESLGPAQWVVVRQVFITRLKEQRYPTRA
                     ELDQAAPSNPVLFATGPDASLNSLALKLSGIDRNFQIDGPGKIEKDPLTGEPTGILRN
                     CTRYVKVSPSDNKPTEQDRERRLIEQFKDYNSVGITSVIDRDAYAPAIARYQKMHDAG
                     ALTVRLGISHHIETLGPLDKILEEIRRVAVHPLRHGGPWLRIVGIKTYLDGGMLTGSA
                     YMREPWGVSKIYAIDDPNYRGVLFIPRDKLVPIVREAVASGLQFTAHTVGDGAVHTLL
                     DTYEEINKSTSVAPTRPCITHSNFMSQEAIDQAARLGVVVDIQPAWLYLDTRTLAAQF
                     GYDRLRYFQPLRSLFAAGVIAGGGSDHMQKIGSLRSINPYNPFLAMEVAITRRAKGYD
                     RPLHPEEALTREQAIRFYTINNAHLLFLEDRIGSLEPGKKADFVVIDRDLLTCPDDQI
                     NSTRALATYLDGKQVFRRTD"
     gene            4716..5081
                     /locus_tag="VHS97_00085"
     CDS             4716..5081
                     /locus_tag="VHS97_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446621.1"
                     /translation="MEEHALAEILNSHFHGETGEKVDSLEVILNVFDIKSVRTTSRND
                     ESGIPIATRGAPRFFQDESALFASINESYTEGFVEVYAPAPLAWADRTKRAKWMNSLK
                     TPIRDTIESIVESTLKGTS"
     gene            5081..>5284
                     /locus_tag="VHS97_00090"
     CDS             5081..>5284
                     /locus_tag="VHS97_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446622.1"
                     /translation="MDAKEFVQLSILAMGSEIQGKTKLQKTVYFLGLMTGCLDDLGYR
                     AHFYGPYSDEVADAIGWLNTIGAV"
BASE COUNT         1066 a         1609 c         1572 g         1037 t
ORIGIN      
        1 tagagcgcca caatctcgag cgtcgcgaaa ggaccgcctt cgatgtcagg gactgccggc
       61 aacgaaccgt cgacggcctt gattcacgaa ggttggaatc atctgaccag ccagcgccca
      121 ttggccgctt ggggaagctg gcagcgtgtg ttacgggcca atcctgattc gcaggcggcc
      181 caacaggcct tggcgactct cgaatccgcc gcggacttac ccctggcggc aaggacttca
      241 tatcgatttc gccagccatc cgacccaacg cggcgaacgg cgtgggacgc ccggatgcgc
      301 gatcagggcg acaacgagct cgaggcaacg gccgacgtgt tcggccggct ggcggccgaa
      361 gatcccaccg attccgccgc ctggtacaac cgggcgctct gtctggcctg gatgggcaaa
      421 aacgtggagg caatcggctg cctggaccgc gtcgtcggcc tcgaggcgga gcgggcgttc
      481 gatctggccg tcgacgcctg gactcttgcc gaagtacttc gccagggagg cggagccgaa
      541 acgctggccg acgacttgcg cttcgcttgc actgttgcct ggaaaccccg cgacacgtcg
      601 tggctgctcg aggagtttcc cgaaattcag cgtattccca ccccgcgcgc accgggagca
      661 gcctcggatg agcgttccga gatcgaggtc tacgagtggt tagaccggcc ggtttccgag
      721 cttactggtc gttcgccagc gcccgccctg ccgccgacag tgctagcgag cgtctatgtg
      781 aagggtcaca cgctgcgcct gtcgagtcca cgggtcgaga ccctggaacg gatcgaggag
      841 gtcctcttcc cacggctcga gattggcggc ggctcggtcc ggcgcgaagc gtcgccgttg
      901 ccgcttccgt tcctcgacgc cgacgtctgg attttccgga ttccaccgga tctcgagccc
      961 gatcacgcca accatctcag ccgcgagtcg gtcgagcggt atttggagga ccagtggatc
     1021 caccgcccgc gccacgggct cgacagccgc tcgccgctgg ctgcctcggt cgcggcgagc
     1081 cgcggtgata cagtcgcgcg cgccaagctg actgcggtca ttcgcctgcg agaacagctc
     1141 ggcaaccgtc ccagcgcgat tctgctctat cagggctatc cttttgatcg gctgcggcgc
     1201 cggcttggtc tggagatggt ctatcccact gcggtcgacg tggaagacct tggctgtgcc
     1261 agcgccgggg aactggatcg ccttgaccca gcgacgctcg atgacacgcg gctcgtcgaa
     1321 gcgttcatgt ccgccgccgg cttgaaggac gacgcgcgga ccgggcgcct ggcgaccgaa
     1381 ctcttgaagc gcaggccgat cgcacttaag tcgctcgacc tcgctctggt ggtcgctccg
     1441 ttggtgcgtc agtctattgc gcgcaaccat atcgatgggg ccgtgagctg gctcaaagaa
     1501 gcgcgttcga ttggcgacct agcgacggca acgactctgg agatctggat tgccgaagtg
     1561 tatgcgcggg cagaccgtcc ggaccgggcg ctcaaaactt atatgcgcct gatcaagccc
     1621 gacgcggccg gcgcggcgtt ggcgctggac gccggcgaga tgatgctcga caacggtcat
     1681 ctcgatgaag ccaaggttct cttgctcttg gcgggagaca tggcacgccg caacgggcgg
     1741 cagtggatcg agcggcgggc gcggcggtta ctcgatcggc tctcttgagg gcgtagttgt
     1801 cattgacggt ctgttcgttt ggacttgcct tagtggggtc gaaaccgcga acatcaagtc
     1861 ttgtgaagtc ggcttacgca ggaaaaatca gaatctcgta tcgcggctgt ttatggcgtt
     1921 tgcaatcgaa ttctcaccgg atgcccgcga ccatgtccga tcactgaaga agcgagacta
     1981 atcgatcata ctcgatgcca tcgcagaaca attgacctat cagcccgacc aggcgaccac
     2041 acgccgaaag cggctggagg ataatcacct cgcgccgtgg gagctacgca tcggtgattt
     2101 ccgcgttttc tacgatgtca acattgatga ccaggtggtt caagtggtag cggtcgggat
     2161 gaaggtgcat gaccggctgc gaatcggtgg cgaggagatt acattatgaa aatcgtaccc
     2221 cttgaaggca caacgatgac ggtcgccgag ttggtcgaat tggcccaggg tgaatccgtc
     2281 attttgacgc gcgaaggcaa tccgctggtt tctgtcaagg gcatcacggg ttccgactgg
     2341 gaatcggcgt cgttggccca caacccacag tttgcggagc ttatcgagcg atcgcgccgc
     2401 tcatatcgcg agcaaggagg cattggtatg gatcaaatgc gtcgggaact tgggctagag
     2461 tcacatccat ccgacgtttc cacggcccac gcttgcaatg aggccgagac tggcgattga
     2521 tgcgaagcgg gccagctttg ttacctcgct gtcgagatca ccagctttgt tacgtcgctg
     2581 tcgagatcaa atgtaaagct cgacgtgagc ggggccgctt tcatgacctg gcaaagttcg
     2641 gtatcttcgc ttcggaagta aacgcgtccg gtggaccgac gtccccgaat cgtggatcat
     2701 cgtaatacca ccgaagaatc gacgcaatcc gactgtcgta caggaggctt agcttgccac
     2761 gagttcgcac catcatgacc ctccgcgccc atgtcgcggc cacttgtctc ttgttcttgg
     2821 tcggaccaaa cggacggact gctttggcca acgccgacgc tgacgtgatt ctccatcacg
     2881 gcaagatcgt cacggccgat cgcgcgttct cgatccacga ggcgctcgcc gtcaagggag
     2941 accaggtgat ccaggtcggc tccgatgcag acgtcctccg gtcgcgcggg ccgaacacga
     3001 ccctggtcga cctcgcgggc aagatggtat tgcccggctt gatcgattcc cacacgcacc
     3061 caaccgacgc ctgcatgatc gagtttgacc atccactacc tgagatggaa accatcgcgg
     3121 atgtgctcaa atatattcat gcgcgggccg aatcgttggg cccagcgcag tgggtcgtcg
     3181 tccgccaggt cttcattacg cgactgaaag aacagcgcta tccgacgcgc gccgagctcg
     3241 atcaggccgc tcccagtaat cccgtgctat tcgcgaccgg ccccgacgcc tcgctcaatt
     3301 cactggcttt gaagctcagc gggattgatc gcaactttca gatcgacgga ccgggcaaaa
     3361 ttgaaaagga cccgctcacc ggcgagccga ctggcatcct gcgcaactgc actcggtacg
     3421 tcaaagtttc cccctcggac aataagccga ccgagcaaga ccgggagcgg cgacttatcg
     3481 agcagttcaa ggattacaat tcggtcggca tcacgtccgt catcgatcgc gacgcctacg
     3541 caccggccat cgctcgctac cagaaaatgc acgacgccgg ggcgctgaca gtccgacttg
     3601 gtatttcgca ccacatcgag acacttggtc cgctcgacaa gatccttgaa gagatccgcc
     3661 gcgtggctgt tcacccgctt cgtcacggcg gaccctggct ccggatcgtc gggatcaaga
     3721 cctatctcga cggcggcatg ctcacgggca gtgcctacat gcgcgagccg tggggagtga
     3781 gcaagatcta cgcgattgac gacccgaatt atcggggggt tctcttcatc ccgcgcgaca
     3841 agctagtgcc gatcgtgcgc gaggccgtcg cgtcgggcct tcaatttaca gcccacactg
     3901 tcggcgatgg ggccgttcac actctgctcg atacctacga agagatcaac aagagtacga
     3961 gcgttgctcc tacgcgaccg tgcatcacgc actctaactt catgagccag gaggccatcg
     4021 accaggccgc ccggctgggt gtcgtggtcg acatccagcc ggcctggctc tacctcgata
     4081 cacgcacgct ggccgctcaa ttcggctatg accggctgcg ctacttccag ccattacgca
     4141 gcctgttcgc ggccggcgtg atcgctggcg gcggctcaga ccacatgcag aagatcggct
     4201 cgctccggtc gatcaaccca tacaatccct tcctggcgat ggaggtcgcc atcacccggc
     4261 gggccaaagg ctatgaccgg ccgctacacc ccgaagaggc cttgacccgc gagcaggcca
     4321 tccgcttcta cacaattaac aacgcccatc ttctgttttt ggaggaccgg atcggctcac
     4381 tcgagcccgg caaaaaggca gatttcgttg tgatcgaccg cgacctcttg acctgtcccg
     4441 acgaccagat caactcgacc cgggcacttg caacatatct tgacggcaaa caagtcttcc
     4501 gccggactga ctgactctcc gtacgagtcg gatgggagct cggaacccgt gcggggccat
     4561 tacgtaccaa agtagtcttg cgtacagtaa cggcgtccgg tagtgtgcgg gcattggaac
     4621 cattgcattg ggggaacaaa agttggccga acggcaccag atccgtgatc ccgttcacaa
     4681 tttcgttgag ttatgggaga aagagatccg actcgttgga agagcatgct ctcgccgaaa
     4741 ttctgaactc tcatttccat ggagaaactg gtgagaaagt cgattcgctg gaagtgatcc
     4801 ttaacgtatt cgatattaag tcggtgcgga cgacctcgcg taacgacgag tccggtatcc
     4861 caatagcgac ccgaggggcg ccgcggttct ttcaggacga gtcggccctc tttgcgtcca
     4921 tcaacgaaag ttataccgaa ggattcgtcg aggtctacgc ccccgcccca ctggcctggg
     4981 cggaccggac gaagcgcgcg aagtggatga actccctcaa aaccccaatt cgcgacacga
     5041 tcgagtcgat cgtcgaatcg acacttaaag gaacgtcatg atggatgcaa aagaattcgt
     5101 acaactttca atcttggcta tggggtccga aattcaaggc aaaacgaagc tccagaagac
     5161 cgtgtacttc ctcggattga tgacgggatg cctagacgat ctcggctatc gtgcgcactt
     5221 ttacggcccc tattcggatg aagtcgccga tgcgatcggt tggctgaata cgatcggcgc
     5281 cgtt
//
LOCUS       DATAQL010000006         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466 k141_546,
            whole genome shotgun sequence.
ACCESSION   DATAQL010000006 DATAQL010000000
VERSION     DATAQL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VHS97_00095"
     CDS             complement(<1..561)
                     /locus_tag="VHS97_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HEX3446623.1"
                     /translation="MGLYCEQGGFFIDPWRAVDRAVVTHAHADHLCWGCGQYLMAQDG
                     LTVARTRLRDEPAITTVPYGEPVDIHGVRVSLHPAGHILGSAQVRLEHAGKVWVVSGD
                     YKVEPDLTCAPFTPIPCHVFISECTFGLPVFRWPVQGDVFAEIFAWWRGNQAAGRSSL
                     LFAYALGKAQRLLAGLAALARADQGLP"
     gene            complement(744..1109)
                     /locus_tag="VHS97_00100"
     CDS             complement(744..1109)
                     /locus_tag="VHS97_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX3446624.1"
                     /translation="MGKKLYVGNLTFSVTNADLEELFAPFGEVRSAQVVEDRQTGRSR
                     GFGFVEMASDADALAAIDALHDQEHNGRRLTVNEAKPREDRGGGGGGGYRGGGGGGGG
                     YRGGGGGGGGGYRGGGDRY"
     gene            complement(1352..2158)
                     /gene="eutC"
                     /locus_tag="VHS97_00105"
     CDS             complement(1352..2158)
                     /gene="eutC"
                     /locus_tag="VHS97_00105"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEX3446625.1"
                     /translation="MEDESRAIERLQSAPEPALVPSPAPTLSLPSLTLAQIRARTPAR
                     VLTGRAGGAYRTATWLELRSDHAAARDAVCDELDLLSDLTTSFVAEWGLFEVSTLARS
                     KAEFLLYPERGRALDEPSRIELTNYCPPGADFQVAIADGLSAAAVRVQVPVLLPLLAA
                     EARRRSWRFGQPFFIRHGRVGVLNDVGEVLQPAVVVLLIGARPGLATAESLSAYMAYR
                     PRAGHDDARRNLISNIHARGISHDQAALRIAHLAARMIEFQTSGFAMKEE"
     gene            2363..>3599
                     /locus_tag="VHS97_00110"
     CDS             2363..>3599
                     /locus_tag="VHS97_00110"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEX3446626.1"
                     /translation="MMRVWYSNQLERLVDRLIENLGTANCSPATRLFTMPTIIVPNRN
                     IETYLKYEIARGAGVAAGLNFKVPEEFLKSLLAREDGEQARKLVSRHALRAFFIEVLS
                     QDSESGQTLPDAVRAYIDAGGDDGDGRDLRRFQLSSRLAQLARQYGDYRPDWLRAWSK
                     GQITLEGDPMEATEQWQRDLWAQLIVLVRAQPGRGADWVLPFELFGSLEKTDWGPANQ
                     VHLFGFSYVWHGLPELIKYLLQGQDVCIYSLAPFVEFQEDLPVLDLQSRNGQPFVRRG
                     AKVLKTTEAGEAHSPADLAIVAQWGRPGREYLGMLGQFPKTDLHPEFVKSDQKTVLGR
                     LHREILARSVESSGPLEPDPSLAIMACPGVRREAEVVANEIWRLIRDDDGRNPPAPDR
                     LRFRDIAVLLADRANQSAYQ"
BASE COUNT          750 a         1101 c         1084 g          664 t
ORIGIN      
        1 gggtaaaccc tggtccgctc gagccaacgc tgcgaggccg gctaacagcc gttgtgcctt
       61 accgagggca taggcaaaca agaggctcga gcggcctgca gcctggttac cgcgccacca
      121 cgcgaagatc tccgcaaaca cgtccccttg caccggccaa cgaaagaccg gcaaaccgaa
      181 cgtgcactca ctgataaaga catgacatgg gatcggagta aacggtgcgc aggtgaggtc
      241 gggctcgacc ttgtaatcac ccgagacgac ccaaaccttg ccggcatgct ccaaccttac
      301 ctgcgctgag ccgaggatat gacccgccgg gtgcagcgag acgcgtactc catgaatatc
      361 aaccggctcg ccgtagggga ccgtggttat cgcgggctca tctcggagcc gagtgcgcgc
      421 cacagtcaat ccgtcctggg ccataaggta ctggccgcag ccccaacaaa gatggtcagc
      481 gtgtgcgtga gtaaccacgg cacggtcgac cgcgcgccag gggtcgatga agaagccccc
      541 ctgttcacag tagagaccca cgtctgtcac tcggagcagt gggttttcgt ccaggtgcat
      601 gtctctatta tagacacgac ccgggcccga caatcacccc cttttttgct gtcgatcgaa
      661 gctgatcgat caggcattgg tttgattcga gcgagccggc gccggcaact cgtgttggat
      721 cgacccagcg gcatcctgcg tggtcaatac cggtcccctc cacctcggta cccgccgccc
      781 ccgccgccac ctccaccccg atacccacct cccccgcccc cgccgccccg ataaccacca
      841 ccaccgccgc caccgcggtc ctcgcgcggc tttgcctcgt tcacagtcaa acggcgtcca
      901 ttgtgctcct ggtcgtgcag agcgtcgatg gcagcgagag catcggcgtc agatgccatc
      961 tcgacgaagc cgaagccgcg gctgcgaccc gtttgccggt cctcgacgac ttgagcactc
     1021 ctcacctcgc caaaaggcgc gaacaactct tccaggtcag cgttcgtgac cgaaaacgta
     1081 agattgccga cgtacagctt cttacccacc gtcaaacccc cccagggtct ggaaactgac
     1141 aaagtgaccc gcagtcgact ttccgggtca aacgaaagag gccgagccgg acggcccatc
     1201 cggtgggcta ggcgattgag gtcgatgacg attctgggct caatgaagcg gagcaaaatt
     1261 ctcgaactca attcgacaag ccgaccgcaa gagtttaacg aggatcgaca cgaaatggta
     1321 ccgattccag gcgtaaaggt gccgttgcct ttcattcttc cttcatggca aagccacttg
     1381 tctggaactc gatcatgcgc gcggccagat gagcgattct taaggcggcc tggtcatgag
     1441 agatgcctcg ggcgtggatg ttggagatca ggtttctgcg ggcatcgtcg tggccggcac
     1501 gtggccggta ggccatgtat gccgataagc tttcggcagt ggccagcccc gggcgtgcgc
     1561 cgatcaagag aacgacgaca gctggctgca agacctcacc gacgtcgttg agcacaccca
     1621 cgcggccgtg gcgaatgaaa aagggctggc cgaaccgcca gctccgacgg cgggcttccg
     1681 ccgcgaggag ggggagcagt acgggaacct ggacacggac ggcggcggcg gatagcccgt
     1741 cggcgatggc cacctgaaag tcggcgcccg gcgggcaata attggtcagc tcgattcgcg
     1801 atggctcgtc cagtgcacga ccccgctcgg gatataggag aaattccgcc ttggagcggg
     1861 ccaacgtaga cacttcaaac agcccccatt cggccacgaa tgatgtggtc agatcgctca
     1921 gcagatcaag ttcatcacaa accgcatcgc gagccgcggc atggtcgctc cgcagctcga
     1981 gccaggttgc ggtgcgatag gcaccccctg cacgcccggt caagacacga gccggagtgc
     2041 gcgctcgaat ctgcgcgagc gtaagggagg ggagcgatag agttggtgcc ggtgagggca
     2101 ccagcgccgg ctcaggggct gattgaaggc gctcgatcgc gcgggattcg tcttccattg
     2161 cagcgattct cgagctcatg tgcgcggaac aagaaagatc tcggttgatt cgagcttccg
     2221 gccgtgtcgc tctgagttta acagacgatg aggatcactt tcattacggc ccaattcttg
     2281 cgataatgta cggatgagcc cttgcttgta aacgggtgct ggcgtgcgcc tagactcgac
     2341 cgcccgggag aaacgatcga cgatgatgcg cgtttggtat tccaaccagc ttgaacggct
     2401 ggttgatcgc ctgatcgaga atctcggaac ggcgaactgt agcccggcaa cacgcttatt
     2461 cacgatgccg acgatcatcg tcccgaatcg aaatatcgaa acctatttga aatacgagat
     2521 cgcgcgtggg gccggcgtgg cggccgggct gaatttcaaa gtcccggagg agtttctcaa
     2581 atcgctgctg gctcgcgagg acggagagca agctcggaag ctagtgagcc gccacgcgct
     2641 tcgggctttc ttcatcgaag tgttatctca agattccgaa tcggggcaaa cgctccctga
     2701 tgcggtccga gcctacatcg acgctggcgg tgacgacggg gatgggcgcg acctgagacg
     2761 gttccagctt agctcgcgtc tggcgcaact tgcgcgacaa tacggcgatt atcggcccga
     2821 ttggctaagg gcctggtcca aggggcaaat cacgctcgaa ggcgatccca tggaggctac
     2881 agaacaatgg cagcgtgacc tctgggcgca actaatcgtg ctcgttcgcg ctcagccagg
     2941 gcgcggcgcg gactgggtct tgccctttga gctatttggt tcgctcgaga aaacggattg
     3001 ggggccggcc aaccaggtgc atctctttgg tttctcctat gtctggcatg ggctccccga
     3061 actcatcaaa tatctccttc aagggcaaga cgtatgtatc tacagcttgg cccccttcgt
     3121 cgaattccag gaagacctcc ccgttctcga tctgcaatcc aggaacggcc agccgtttgt
     3181 tcgacgaggc gcgaaggttc tgaagacgac tgaggctggc gaagcacatt caccagccga
     3241 ccttgccatc gtcgctcaat ggggccgccc agggcgagaa tacctgggga tgctcgggca
     3301 attccctaag acggacttac atcctgagtt cgtgaaaagc gatcaaaaga cggtgctagg
     3361 ccgccttcat cgcgagatcc tagcgcggtc ggtcgagagt agcgggccgc tcgaacctga
     3421 cccaagtctg gcgatcatgg cttgccctgg cgtccggcgc gaggccgagg tcgtcgccaa
     3481 cgagatctgg cgtttgatcc gtgacgacga cggccgcaac ccaccggcac cggatcggct
     3541 gcggtttcgc gatatcgccg tgctgttggc ggaccgggcc aatcagtctg cctaccaag
//
LOCUS       DATAQL010000007         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2901444, whole genome shotgun sequence.
ACCESSION   DATAQL010000007 DATAQL010000000
VERSION     DATAQL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901444"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1619
                     /locus_tag="VHS97_00115"
     CDS             186..1619
                     /locus_tag="VHS97_00115"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX3446627.1"
                     /translation="MNDPDSSPLLTCPECGKLRVSLDEARLQIAQLQTELRDLRAQLN
                     RNSSNSSTPPSADPPGAPKPVVKTPTGRKPGGQPGHRGHHRQRLPPERINTIVPYLPT
                     TCAHCQAPLPAEPGPGDPEPTWHQVAELPELAAVLTEHQGHARTCPCCGGLNRGAIPP
                     EIRAHVIGPRLAAVMSYFSGRHHIGRRGVEEVVETVFEVPISLGSVFALERETSAALA
                     SPYHEAQAAVRDAPVKNTDETGWSENGQKRWLWGAATATVAFFVIHLRRNFEGLQALL
                     GETITGIVGSDRWAAYSKLPLELRQICWAHLKRDFQKLIDRGGPAEAIGRVGLEVVEC
                     LFADWWAFRRGELDRPGLQARLDPIARELQAVLEQGRGCADSKAATFCANVLALYPAL
                     WLFAAIEGVEPTNNHAERILRLGVLWRKNAFGCHSPAGCRFVERMLTVVQTLRLQKRP
                     VLDYLHRAIAAHRAGLPAPRLLSQDGN"
BASE COUNT          319 a          569 c          538 g          318 t
ORIGIN      
        1 ctcggaacac tcagatcagt tcgcggtcag tggaatcgag acattgttag gacccgcaga
       61 agcgccgtca agcagattct ttaaatgtaa ccgatcagtc cactccttgg catcgataat
      121 gctagggttg caagaatcaa ccaggtgctt gcaagttccg gtcgttcctg tgtagcgtct
      181 cactcatgaa cgatccggat tcgagccccc tgcttacctg ccccgagtgc ggcaaacttc
      241 gtgtctcgct cgacgaggct cggctccaga tcgcccagtt acaaaccgaa ctccgcgacc
      301 ttcgcgcgca actcaatcgc aattcctcga actcctccac acctccttcg gccgatcccc
      361 caggggcgcc caagcccgtg gtcaagacgc cgacggggcg aaaaccaggc ggacagccag
      421 ggcatcgggg acatcatcgc cagcgcctcc cgccggagcg gatcaacacc atcgtgccgt
      481 atctgcccac gacctgcgcc cactgccaag cccccttgcc cgccgagccc gggccgggcg
      541 accccgagcc gacctggcat caggtcgccg aactccccga gctcgcggcc gtcctcaccg
      601 agcaccaagg gcacgcgcgg acctgcccct gctgtggcgg cctcaatcgg ggcgcgatcc
      661 ccccggagat ccgcgcccat gtcatcgggc cacgcttggc cgcggtgatg tcttacttca
      721 gcggccgtca tcacatcggc cgccggggcg tggaagaagt cgtcgagacc gtctttgagg
      781 tgccgatctc cctgggctcc gtcttcgcgc tcgaaaggga gacgagtgcg gccctggcct
      841 ctccgtatca cgaggcccaa gcggcggtcc gtgacgcccc ggtaaagaac accgacgaga
      901 ccggctggag cgagaacggg cagaaacgct ggctctgggg tgcggcgacc gcgacggtgg
      961 ccttcttcgt gattcacctc cggcgcaact tcgaaggact tcaggccctg ttgggcgaga
     1021 cgatcaccgg catcgtcggt agcgaccgct gggcggccta cagcaagctg cccctggaac
     1081 tgcgccagat ctgctgggcc cacctgaaac gcgactttca gaagctgatc gatcgaggag
     1141 gacccgctga agcaattggc cgagtgggtc tggaggtcgt ggaatgcctg ttcgccgact
     1201 ggtgggcgtt ccgccgcggc gaactggacc gacccggcct gcaagcccgg ttggacccga
     1261 tcgcgcgcga actccaagcg gtcttggagc agggtcgcgg ctgtgcggat tccaaggcgg
     1321 cgaccttctg cgccaatgtc ttggcgttgt atccggcctt gtggttgttc gcggcgattg
     1381 aaggggtcga gccgacgaac aaccacgcgg aacggatcct gcggttgggc gtgctgtggc
     1441 ggaagaacgc cttcggttgc cacagcccgg cggggtgccg gttcgtggag cggatgctga
     1501 ccgtggtcca aaccctgcgg ttgcagaaac gtcccgtgct ggattatctc catcgcgcca
     1561 tcgcggctca tcgcgccggc cttcccgctc cgcgattgct gagccaagat gggaactgat
     1621 cggttacgaa agtggtacct gatatattgg gcgttgaagg tctcgctcca agcttagtta
     1681 aacatcggaa gtcactcatt aaaggatcat cagggtccaa tcgcgattcg tctgatctcg
     1741 ggct
//
LOCUS       DATAQL010000008         4892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4886390, whole genome shotgun sequence.
ACCESSION   DATAQL010000008 DATAQL010000000
VERSION     DATAQL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4886390"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VHS97_00120"
     CDS             complement(<1..1281)
                     /locus_tag="VHS97_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3446628.1"
                     /translation="MDHDLDQPRYKPANGPMPARERPPAALDLDKILPVTSSARPKWQ
                     GLKGLLVAQIFGQFNDQAFKQIVILLAMATVASEAEKQERTAFVSMMLLIPLTIISLP
                     AGVLADRVSKRSVIIAMKVFEMILMLVGAAALYMQPHGGWISIGVLGLIGVQTAFFVP
                     AKYGILPEILPHERLSAGNGLLEMTSNLAALAGIVGGGIILFAVGIHTWLGGVILAIL
                     SAFGLWASLTIPPVKAARAEGGVGKTARLAWESIHADRVLGLALLGQVFVWTIATMVP
                     PVVLAYDASHLGLKDWQSGLPLAALGIGIGVGCLLAGKLSASKVEYGLLPLGALGLTL
                     SALVFAAIGPGLYGTIVVMTFLGIFSGLLFVPLNALLQWRSPADRRGAVISMANLLVY
                     GGMVLGTFLALVLARAGVSARGTFLAASIVLAGGF"
     gene            1662..2648
                     /locus_tag="VHS97_00125"
     CDS             1662..2648
                     /locus_tag="VHS97_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3446629.1"
                     /translation="MEQRQLGKGGPLVSAIGLGCMGMSDFYGPADDTESIATIHEAVD
                     RGINFLNTGDFYGMGHNEMLIRQALAGRRDRVFISVKFGALRTPRGEFVGFDARPQAV
                     KTFLAYSLKRLGTDYIDLYQPARVDPAVPIEETVGAIADLVKAGYVRHIGLSEAAGAT
                     VRRATAVHPIAALETEYAVVTREIETETLPTVRELGVAVVAYGVLSRGLIGGSSAGSF
                     NAPGDSRSLVLPRFQGENLRKNLALVDALAAVAAEKGVTVAQLAIAWVLAQGQDIIPL
                     VGARRRDRLAEALGALDVHLSADELARIDQSVPAGAVAGDRYPPEAMHSVNR"
     gene            complement(2669..3799)
                     /locus_tag="VHS97_00130"
     CDS             complement(2669..3799)
                     /locus_tag="VHS97_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HEX3446630.1"
                     /translation="MIDPDRARVSQPRRMALAVWAAVLMVVATPGCETRISREETTAR
                     PHQEADTRPFERLSQYKLFAGEPKDQQPASSVIPYDLNSALFSDYADKYRFVKLPAGT
                     HATYSDDGVFVFPVGTVIAKTFAFPRDARDPKAGRRLIETRILKHDADGWVGLPYIWN
                     AEQTDAILDVAGDTVDVSWIHTDGRTRTNNYIIPNANQCKGCHKAGESMTPIGPRARH
                     LNRDFTYQEGAENQLAHWSRLGALVGATDPGAAPRLANWADQESGSLDARARAWLEIN
                     CAHCHNPEGPARNSGLDLLASQHNPTAFGIEKPPIAAGNGSGGLLHDIVPGQPDKSIV
                     VYRISSTHPGVMMPELGKRLVHDEGVALIREWIAAMPVASRN"
     gene            complement(3796..>4892)
                     /locus_tag="VHS97_00135"
     CDS             complement(3796..>4892)
                     /locus_tag="VHS97_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HEX3446631.1"
                     /translation="DFKSTLSLDVSHVTLKGQGADKTILSFKNQGAGTGGEGLSITSK
                     DDVTLADLAIEDARGDGIKVNGTKRIIVRNIRAEWTGGPKETNGGYGIYPVLCTDVLI
                     EGCTVAGASDAGIYVGQSTNIVVRRNTAEKNVAGIEIENSNKADVYQNWATDNSGGIL
                     VFTMPDLPTKDGLACRVYSNKVLANNHANFAPKGNIVATVPPGTGIMIMANDQVELFD
                     NTIEQNQTAGLSIVSYLLTERPLKDEKYDPYCEAIHVYGNHFARNGTKPAGPIGEMLA
                     KALGSPLPDILYDGIIDPKKQVGGKLPDALAVRIHNNGDARFVNFDAPALTAAAAGSG
                     NGKVPHFVRDLKEYAGTLPSLAPVSIEGLQ"
BASE COUNT          908 a         1506 c         1513 g          965 t
ORIGIN      
        1 gaaaccaccg gccaggacga tcgatgccgc caggaacgtg cctcgtgccg agacccccgc
       61 acgggccagc acaagcgcca ggaaggtgcc cagaaccatg cctccatata ccaagaggtt
      121 ggccatcgag atcacagcac cgcgacggtc ggccggcgac cgccattgca gaagcgcgtt
      181 aagcggaaca aagagcaagc cgctgaaaat ccccagaaag gtcatcacaa cgattgtgcc
      241 gtatagccca gggccgatcg cggcgaatac gagcgcgctc aacgtcagac cgagtgcgcc
      301 caaggggagc aagccatatt cgaccttcga tgccgacaac ttgcccgcca agaggcaacc
      361 cacaccaatg ccgattccaa gcgcagccag cggcaggccg ctctgccagt ctttgagtcc
      421 caggtgcgac gcatcgtaag ccaggaccac aggcgggacc attgtggcga tcgtccacac
      481 gaaaacctgg ccgagcaagg cgaggccgag tacccgatcg gcatggatcg attcccaggc
      541 cagccgagcc gtcttgccga cacctccctc cgcccgcgcc gccttgacag gtggaatcgt
      601 caacgacgcc cacaggccga aggcggacag aattgccaga attacgcccc ccagccacgt
      661 gtgaatgccg accgcgaaga gaatgatccc gccccccacg attccggcca gcgcggccag
      721 gttcgacgtc atctccaaga gtccattacc ggccgacagg cgctcgtgag gcaagatttc
      781 gggcaagatc ccgtacttgg ccggcacaaa aaacgcggtc tggactccga tcagacccaa
      841 cacgccgatc gagatccaac caccatgggg ctgcatatac aaggcggccg cacccaccag
      901 catcaggatc atctcgaaca ccttcatcgc gatgatgacc gaccgcttac tgacccggtc
      961 ggccaagacg ccggccggca gcgagatgat cgtgagcggg atcaagagca tcatcgagac
     1021 gaaggcggtt cgctcctgct tttcggcttc gctggccacg gttgccattg ccagcaagat
     1081 cacgatctgc ttgaaggcct ggtcgttgaa ctggccgaag atttgtgcga ccaaaagacc
     1141 tttgagacct tgccattttg gtcttgcgct gctggtcacg gggaggatct tgtccagatc
     1201 cagggccgcc ggcggccttt cccgagccgg catcgggccg ttcgccggtt tgtaccgcgg
     1261 ttgatccagg tcgtggtcca tctcttcgat cccttctcag cgacccgcct tgggtctcgt
     1321 gcgcatctcg cgtgcgaaag tgatgttcga cgtctcctgg atctttcatg accgattgca
     1381 cgcaaattgg gccatccttg ccccaatatc ctcaatcgca cacgttcact ctaggtcgtt
     1441 ccccggcaac ctggcacgcg ctggccgaaa tgctcgacga agtccctctt ctcacgagcg
     1501 agaagcggtt cgcagttttg tcacacggat accgcattca gtaagctttt tttcagtccg
     1561 tcgttgagga tgcaagctcg tgtcgagtaa cctatttccg ttgtattgat agactgtctt
     1621 cgaagatcga atccacggct cgagtaaaaa gggcgaattt cgtggaacaa cgccagctcg
     1681 gcaaaggtgg tccacttgtc tcggcgatcg ggctaggttg catggggatg tcggactttt
     1741 acgggcccgc cgacgacacc gaaagcattg cgacaatcca tgaggcggtc gaccgcggca
     1801 tcaacttcct caacacgggc gatttttatg gcatgggcca taatgaaatg ctaattcgcc
     1861 aagccctggc gggccggcgc gaccgcgttt ttatctcggt gaagttcgga gcgctccgga
     1921 ctccaagggg cgagttcgtt gggtttgacg cacggcctca ggccgtgaag actttccttg
     1981 cctacagcct caagcggctt ggcaccgatt atatcgacct gtaccagccg gcccgcgtcg
     2041 acccggcagt tccgatcgaa gagaccgtgg gcgcgatcgc cgatctcgtc aaggccggct
     2101 acgtccgcca catcggcctg tcggaagccg cgggggcgac tgttcgccgc gccaccgcgg
     2161 tccacccgat cgccgccctc gagaccgaat atgccgtcgt cacgcgcgaa atcgagaccg
     2221 agactctacc gacagtgcgc gagttgggtg tagctgtcgt cgcctacgga gtcctttcgc
     2281 gcggcctgat cggaggctca tccgccggga gtttcaacgc acccggcgat tcgcgctctc
     2341 tggtcctccc gagattccag ggtgaaaacc tcaggaagaa cctcgcgctc gtagatgcct
     2401 tggccgccgt cgcggccgag aagggggtaa ctgtagcgca acttgccatt gcctgggtgc
     2461 tggcccaggg acaagacatc atccccttgg tgggagcccg ccggcgcgac cggctggcgg
     2521 aagcactcgg ggcactcgac gttcacctct cggccgacga gctcgcgcgg atcgaccagt
     2581 ccgtaccggc cggcgcggtc gccggcgatc gatatccacc tgaggccatg cactcggtca
     2641 accgctaaac cgtcccctta tccgccggct aatttcgcga cgccactggc attgccgcga
     2701 tccactcgcg gatcaacgcc acgccttcgt cgtgcacgag ccgtttgccc aattcgggca
     2761 tcatgacgcc ggggtgtgtc gaggagatcc ggtaaaccac aatcgatttg tcgggctggc
     2821 cgggaacgat atcgtgcagc aggccgccag acccgttgcc ggcggctatc ggcggtttct
     2881 cgatgccgaa cgcggtcggg ttatgctgcg atgccagtag gtccagaccc gagttgcgcg
     2941 cggggccttc ggggttgtgg caatgagcac agttgatttc gagccatgcc cgggcgcggg
     3001 cgtcgaggct gccgctctct tgatcagccc agttggcaag ccgcggtgcg gcgccagggt
     3061 cggtggcacc gacgagcgcg cccaagcggc tccagtgtgc gagctggttt tcggcccctt
     3121 cttgataggt gaagtcgcgg ttcaagtggc gcgccctggg gccgatcggc gtcatggact
     3181 cgccggcctt gtggcatccc ttgcattgat tggcgttggg aatgatgtag ttgttggtcc
     3241 gggtacggcc gtcggtgtga atccagctca cgtcgaccgt atcgccggcg acgtcgagga
     3301 tggcgtcggt ctgctcggcg ttccagatgt aaggcaggcc cacccagccg tcggcgtcat
     3361 gcttgaggat ccgggtctcg atcagccgcc ggcctgcttt ggggtcgcgc gcatcgcgcg
     3421 ggaacgcgaa ggtcttcgcg atcacggtac cgactggaaa cacgaagacg ccgtcatcac
     3481 tgtaggtcgc atgggtaccg gcaggtagct tgacgaacct gtatttatcg gcgtagtcgc
     3541 tgaacagcgc cgagttgagg tcgtaaggaa tgacgctgga tgcgggttgt tgatccttgg
     3601 gctcgcccgc gaaaagcttg tattgcgaaa gcctttcgaa ggggcgtgtg tctgcctctt
     3661 ggtgcgggcg tgcggtcgtt tcttcgcgag aaatgcgcgt ctcgcaaccg ggagtcgcga
     3721 ccaccatgag gacggcggcc cagacggcca aagccattcg acggggctgg ctcacgcggg
     3781 cgcggtcggg atcgatcact gcaatccctc gatcgaaaca ggcgccaggg aggggagggt
     3841 acccgcatat tcctttagat cgcggacgaa gtggggcacc ttcccgtttc cggatccggc
     3901 tgcggccgcc gtcagggcag gggcatcgaa gttgacgaat ctggcgtcac cgttgttatg
     3961 aatccgcacc gccagcgcat cgggcaattt gccgccgacc tgtttcttgg ggtcgatgat
     4021 cccatcgtac aggatatccg gcagaggcga gcccagggct ttggccaaca tctcgccgat
     4081 tggcccggcg ggcttggtgc cattgcgggc gaagtgatta ccataaacgt ggatcgcctc
     4141 gcagtacggg tcgtactttt cgtccttgag cggccgctcg gtaagcagat agctgacaat
     4201 cgagaggccg gcggtctggt tttgttcgat cgtattgtca aaaagctcga cctgatcgtt
     4261 cgccatgatc atgatgccgg tgccgggcgg gacggtcgcc acgatgttcc ctttgggcgc
     4321 gaaattggcg tgattgttgg ccagcacctt gttgctgtac acgcggcagg cgaggccatc
     4381 cttggtgggc aggtcgggca tcgtgaagac gaggatgccc ccagagttgt cggtcgccca
     4441 attttggtag acatcagcct tgttcgagtt ttcgatctcg atgcccgcga cgtttttctc
     4501 ggccgtattg cgccgaacga cgatgttggt ggattggccg acgtagattc cggcgtccga
     4561 ggcgccagcg actgtgcagc cttcgatcag cacgtcggtg caaagaaccg ggtagatacc
     4621 gtatccgccg ttggtctctt ttggaccgcc agtccactcg gcccggatgt tccgcacgat
     4681 gatccgcttg gtcccattga ccttgatgcc gtcgccgcga gcgtcttcga tggcgaggtc
     4741 cgccagggtc acgtcgtcct tgcttgtaat ggacagtccc tcgccgcccg tgccagcccc
     4801 ttggttcttg aagctcagga tcgtcttgtc ggcgccctgc cccttcaagg tcacgtggct
     4861 cacgtcgagt gagagggtgg atttgaagtc ga
//
LOCUS       DATAQL010000009         9396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3359704, whole genome shotgun sequence.
ACCESSION   DATAQL010000009 DATAQL010000000
VERSION     DATAQL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3359704"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VHS97_00140"
     CDS             complement(<1..482)
                     /locus_tag="VHS97_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX3446632.1"
                     /translation="MNASRVEICVVTIALLTSCGRADEPVDSVLDALVAAHNHERELA
                     HVAPLKLSRTLCQSAADHASDMAKQHKLDHKGSDGSTVADRAKRKGYAFVRVGENIAQ
                     GQKSVEKVMDIWMKSPGHRANILADYTEMGAAQAEDDNGDPYWCVNFGIPIPRLNPDE
                     A"
     gene            complement(527..1318)
                     /locus_tag="VHS97_00145"
     CDS             complement(527..1318)
                     /locus_tag="VHS97_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3446633.1"
                     /translation="MNRVTEPALFILTILSFGTCVAQAQVQEAVEQYPSGETRVTVEC
                     FSPDAKGKYPAVFLLHGAGGLDPGTATAFREIGRGLAAQGYVVLIPHYFEKTGHKFGT
                     RFLAGEIQSYDDALHDGIEFAAASGIVDPERIGLVGYSLGAHLAFFRSTRDPRVKAIV
                     SCSGSLPVGWNAKFPPVLILQGSKDKGSPTQRLKAFEEKLKTTDTPYATHVYHWLGHN
                     FDVSTWDDAGRRMAVFFNKHLKKREPKKSKAKSKPQTKTDSSDDS"
     gene            complement(1343..1687)
                     /locus_tag="VHS97_00150"
     CDS             complement(1343..1687)
                     /locus_tag="VHS97_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX3446634.1"
                     /translation="MSAVSGTLPVIESCDGCGACCMVVTRPPFYHVFEDMGEEAWDRL
                     ERERPNLVAELRADYQARQESGGPFYGTPCIWFDAETLRCRHYDYRPLACQMFEVGDE
                     DCRDARRRAAVL"
     gene            complement(1684..2055)
                     /locus_tag="VHS97_00155"
     CDS             complement(1684..2055)
                     /locus_tag="VHS97_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446635.1"
                     /translation="MRLCNTSYRAGLIQGKLRDRSRAAEGRPSAIGNEPVDECVPGFA
                     LERELDIKAGFNFGLLAANKCWVAGVELATASEPPARKPRIWGRRPSGVDPSHPVRCN
                     LLLNFADINSSLRSFQQDMHS"
     gene            2189..2923
                     /gene="lipB"
                     /locus_tag="VHS97_00160"
     CDS             2189..2923
                     /gene="lipB"
                     /locus_tag="VHS97_00160"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX3446636.1"
                     /translation="MRPTPQLPTLEVYMLGLVDFTEVQQLQRRIVYELGEQGGAVLIL
                     CEHPPTISVGRSGSRAHIGPDDETLHALGIKVHWVNRGGGCVLHLPGQLAAYLTLPLD
                     TCGLSLQDYLDRLHRAIMLVLKEFDLAGAVRADLPGVFSGPARIATVGVAVSRSIAYH
                     GLTLNVGPYLEPFDVLEEPGIGNALLRNTSMEARRQRHTSMSKVREALIRCLEQVLGL
                     ERHHVYTHHPLIRRKVHSHVYVPSPG"
     gene            2898..3920
                     /gene="lipA"
                     /locus_tag="VHS97_00165"
     CDS             2898..3920
                     /gene="lipA"
                     /locus_tag="VHS97_00165"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX3446637.1"
                     /translation="MSTFPVPGKSSGLPSLPVLDEEFRSPGSADLVAASAIRSHVRRL
                     PEWLKRPIPVAGGMAFTRSLVSELGLETVCESARCPNRSECWTRRTATFMILGDVCTR
                     PCGFCAVKRGRPDEVALDEPERLAEACHRLGLRHVVITSVTRDDLADGGADHFRQCVL
                     AVRRRTGATIEVLTPDFDGRGELIDVVLSARPEVFNHNLETVARLQQHVRRKSQYQVS
                     LSVLEHVKMTCPQIRTKSGLMLGLGETTEEVLETLADLRAIGCDLLTLGQYLQPSPRH
                     LPVVRYLPPAEFDELGRLARGLGFADVASGPFVRSSYHADEMARLELMPGAELQLSSG
                     NGAGSS"
     gene            complement(3917..5032)
                     /locus_tag="VHS97_00170"
     CDS             complement(3917..5032)
                     /locus_tag="VHS97_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HEX3446638.1"
                     /translation="MVDPHHLVNNASELLSPSLLIYPRLIRHNLHEMISLAGGAGRLR
                     PHVKTHKMADVVRLIESSGIRKHKCATIAEAEMVAAAGGTDVLLAYPLTGPNLKRFVY
                     LARGYRSTTFRATVDNSEAARALSAAAEGLGAPVPVLVDLEIGMGRTGIQPGDAAAEL
                     YALIDRLPNLVPDGLHAYDGHIHETDLDKRRQEAQSGLESTLALRERLLKRGLPVPRL
                     VVGGTPTLPIHAKLDLPGLECSPGTTVLHDNSYLTRYPDLPFIPAALLLTRVVSHPSA
                     GRVCFDLGYKAVASDPVGPRARFLSLDDARPVVHSEEHMVVETSHAESLPVGTPLFAI
                     PTHVCPTVALHRRAYVVEDGEVVGQWEVTARDRVLGV"
     gene            complement(5188..6276)
                     /locus_tag="VHS97_00175"
     CDS             complement(5188..6276)
                     /locus_tag="VHS97_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX3446639.1"
                     /translation="MTADPHSTESVSEPEFARLTRSDRLLAVLSQFRRVVVVSHVNPD
                     PDSLASMLGIKALVESAQPGRPVILTVDGMIARSENRAMVELIPVPLVPVETVTTDSE
                     TAVVMVDTQPFTGRRYSEAAMPQVVIDHHETGGVLQGVLFSDIRTHMGATSTMIAGYL
                     IEQRVVVTPQLATALLYGIDSETTGYPREASSLDDGALIWLFPRADKDLLARIRNPKL
                     PQSHFATFQHALANAFIYDKLIVSWCGAVSQPDIIAEIADFFIRFDQVDWSLAAGVFD
                     DKLKLSLRAGTLGGRAGETLHHVVNGLGSAGGHDKRAGGSIVAADLRPETVESLLRTV
                     RSRLLDELHIDGQQGRRLLNSCPVIPAP"
     gene            complement(6380..7186)
                     /locus_tag="VHS97_00180"
     CDS             complement(6380..7186)
                     /locus_tag="VHS97_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX3446640.1"
                     /translation="MSSRVLLALFDSMYVIALTAWVGSILFFSFGVAPIIFGVLGEQM
                     GGKFVRALFPRYYLWGAIAGAIALPAFVAGPLCYHEFRGPMVGVQALVILAGILCMLY
                     GGNSLTPAINQARDAGPAGRERFNQLHRRAVRLNGLVLLAGIGLLIAFAARPAPRTAG
                     IVELTPAERVRFDNGVNQVIQDVEAKYGLRPPRKHEPGESAERDPLIDDETVDEIESF
                     YARKRARDQARARRGSATDSRAVQGRVSPHMTLPSASESDATRPPGSGQR"
     gene            7601..8374
                     /locus_tag="VHS97_00185"
     CDS             7601..8374
                     /locus_tag="VHS97_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446641.1"
                     /translation="MRPALLLLVALPVLSGCANRQLSFSTLRQTSTLADIQETQVIEN
                     FARLAANPGALPFLSLANSGSATVTDGGSGSLSFLGAHRLFTESTIGVSANRSIALNW
                     GLAPLNNPDRLKAMKAAYLSVLDPSLVDPAEQTKLNDVIKADPSYAINPGWMEVGGKW
                     DVPKHAWRVGRCGKTYVWVMPDRAEDFSRFVLLTLNLASLQSTGKSPLAQGHVGPSAV
                     APPTPSAAAPAADPFPPRLYDQPPSVNPGLFFVPRGTAP"
     gene            complement(8404..8988)
                     /locus_tag="VHS97_00190"
     CDS             complement(8404..8988)
                     /locus_tag="VHS97_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX3446642.1"
                     /translation="MTIKFFKSSSDFRKWLEANHARAPELWVGFYKKTSGRSSITWPE
                     SVDQALCFGWIDGIRKSIDDISYKIRFTPRKPTSVWSTVNVRRALALTNQGLMHSKGL
                     EAFEKRKDNRSGIYSYEQRTDAMPEPYAQKLRANKAAWDFFQTQVPSYRKVVSWWVVS
                     AKKEETRQKRLDKLIEDSAQGRTIDQYTRLQKSK"
     gene            complement(9034..>9396)
                     /locus_tag="VHS97_00195"
     CDS             complement(9034..>9396)
                     /locus_tag="VHS97_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446643.1"
                     /translation="RNLKPELAQGGQVTDGKIHATDYITLQADAYRTSVANIAKMKVK
                     YDMDIYEALNGSKPKSYEEFMEVIIKKGKADGIQLPMLPYYQEYGYDPDKKELVVIEY
                     PAKKKAMEEQSDREHGRN"
BASE COUNT         1818 a         2845 c         2822 g         1911 t
ORIGIN      
        1 gcctcgtccg gattgagccg cggaatggga atcccaaagt tcacgcacca ataggggtct
       61 ccattgtcgt cttcggcctg ggctgctccc atctcggtgt aatcggccag gatattggcg
      121 cggtggccgg gactcttcat ccagatatcc atcacctttt cgacgctctt ctggccctgg
      181 gcgatattct cgcccacgcg aacaaaggcg taacccttgc gtttagcccg gtccgccacg
      241 gtcgagccgt cgctgccctt gtggtcgagc ttatgttgct tggccatgtc gctggcgtgg
      301 tccgcggccg actggcacag cgtacgcgac agcttgagcg gggcgacgtg cgccaactca
      361 cgttcgtggt tatgagcggc cacgagggcg tcaagcaccg agtcaacagg ttcgtctgct
      421 ctgccgcagc tcgtcaggag cgcgatcgtc acaacacaga tttcgaccct gctcgcgttc
      481 atatgagctt acccatcaag ttctgttcac gcgaatttcg cgtctgttac gaatcgtcgg
      541 agctatccgt tttggtctga ggtttcgact tggccttcga tttcttgggt tcccttttct
      601 tcaggtgttt attgaagaat accgccatcc gccggccggc gtcatcccag gtagaaacgt
      661 cgaaattgtg acccaaccag tgataaacgt gagtcgcata aggggtgtcc gtcgtcttca
      721 gcttttcctc aaacgccttc aaacgctgtg tcgggctgcc tttgtccttc gatccctgga
      781 ggatcaacac cggaggaaac ttcgcattcc acccgacggg caacgacccg gagcacgaga
      841 caattgcctt gacgcgagga tcgcgtgtcg accgaaaaaa cgcgagatgg gcccctaagg
      901 agtagccgac gagcccgatc cgctccgggt cgacaatccc gctcgccgcc gcgaactcga
      961 taccatcatg cagggcgtcg tcgtaggact gtatttcgcc ggcaaggaaa cgcgtgccga
     1021 acttgtggcc ggtcttttcg aaataatggg ggatcaatac aacatatcct tgtgcagcga
     1081 gtccccggcc gatctcacgg aaagcggttg cggttcccgg atcgagcccc ccggcgccgt
     1141 gcaacaagaa gactgccggg tatttaccct tcgcatcggg cgagaagcac tcgaccgtga
     1201 cccgcgtttc gccgctggga tactgttcga ccgcttcttg cacctgggct tgcgctacgc
     1261 atgttccgaa cgagaggatg gtcaggatga aaagagcggg ttctgtgact cgattcatgc
     1321 cgggtccctt tccggactac gttcaaagga ccgccgcccg acgtctagcg tcgcggcaat
     1381 cctcgtcgcc gacctcgaac atctggcatg cgagtgggcg gtagtcgtaa tggcggcaac
     1441 ggagcgtctc ggcgtcaaac cagatgcagg gggtaccgta aaacgggcct ccgctttcct
     1501 gtcgggcctg gtaatcggca cggagctcgg caacgaggtt agggcgctcg cgctcgagtc
     1561 gatcccaggc ctcttccccc atgtcttcaa agacatgata gaaagggggg cgcgtcacga
     1621 ccatgcagca tgcgccacaa ccgtcacatg actcgatgac aggcaatgtc cccgatacgg
     1681 cgctcatgag tgcatgtctt gctgaaagga tcgcaatgag gagttgatgt cggcaaagtt
     1741 caaaagcagg ttacacctca cggggtggct ggggtcgacg cccgaagggc gacgccccca
     1801 gatccgaggc ttccgcgccg ggggctcgct ggctgtcgcc agctcgaccc cagccaccca
     1861 gcacttgtta gcggccaaga ggccaaagtt gaaccccgcc ttgatgtcga gttcccgttc
     1921 gagcgcaaac cctgggacgc actcatcaac gggttcgttt ccaatagcag acgggcgtcc
     1981 ctccgctgca cgcgaacgat ccctcagttt accttggatc agtcccgccc gataggacgt
     2041 gttgcaaagt ctcattccgt cttcttatcc cggcttaacc cgcacttgcc tgagggggtt
     2101 tttcgggtgt agagtgaatg gaaatgcaaa ccgtcctgaa ggcgatggag ttcgagtcga
     2161 ctcattttct ggcgattatt gactgacaat gaggccaacc ccgcaactgc cgacgctcga
     2221 agtttacatg ctggggctgg tcgactttac agaggtccag caacttcagc gtcgcatcgt
     2281 ctacgaactg ggcgaacaag ggggggcggt tctgatcctg tgtgagcatc caccgacgat
     2341 cagtgtgggc cgttctggga gccgtgccca catcgggccc gatgacgaaa cgctccacgc
     2401 cctgggaata aaggtgcact gggtaaatcg gggcggcggt tgcgtgctcc atttgcctgg
     2461 tcagctcgca gcctatctca ctctgccgct cgatacttgc gggctgagcc tgcaagatta
     2521 cctcgaccgg ctccatcgcg cgatcatgct ggtgctcaag gagtttgatc tcgccggtgc
     2581 cgttcgtgct gatttgcccg gcgtcttctc gggtccggcc cgaatcgcca cggtcggcgt
     2641 ggccgtcagc cgctcgatcg cctaccacgg attgacgctc aacgtgggtc cgtatctcga
     2701 gccttttgac gtcctggaag agccgggaat cggcaacgct ttgctccgaa acacctcgat
     2761 ggaggcccgg cggcaacggc acacctcaat gtccaaggtc cgcgaggcgc tgatccgatg
     2821 cctggaacag gtcttggggc tggaacgaca ccacgtttat acccatcacc cgctgattcg
     2881 ccggaaggtc cattcgcatg tctacgttcc cagtcccggg taaatcatca ggcttgccca
     2941 gtttacctgt gctcgacgag gaatttagat cgccggggtc cgcggatctg gtggctgcgt
     3001 cggccatacg ctcccacgtg cgccgcttgc ccgagtggct caagcggcca attcccgttg
     3061 cgggggggat ggcatttacc cgcagcctgg tcagcgagct cgggctggag accgtttgtg
     3121 agagcgctcg ctgcccaaac cgctcggagt gctggacgcg gcgtacggcg accttcatga
     3181 tcctggggga cgtttgtact cggccgtgcg gcttttgcgc tgtcaagcgc ggtcggcccg
     3241 acgaagttgc cctcgatgag ccggagcgac tggccgaagc gtgtcaccgg ctgggtctgc
     3301 gccacgtggt gatcacatcc gtgacgcgtg acgacctggc cgacggaggt gccgaccact
     3361 tccgccaatg cgttctggcg gtgcgccggc ggaccggtgc caccattgaa gtgctcacgc
     3421 ctgatttcga cgggcgaggc gagctgatcg atgtcgtcct ctccgcccgt cccgaggttt
     3481 tcaaccacaa cctcgagacg gttgcccgtc tccagcagca cgtcaggcgc aagagccagt
     3541 atcaggtcag cctaagcgta ctcgaacatg tcaaaatgac ctgtccgcaa atccgcacca
     3601 agagcggcct gatgctcggg ctgggtgaaa cgaccgaaga ggtcctcgaa actctggccg
     3661 acttgcgggc gatcggttgc gatctcctca cgcttggcca ataccttcaa ccctcgccgc
     3721 gccatttgcc agtggtgcgc taccttccgc cggccgaatt cgacgagctg ggccggctgg
     3781 cgcgggggct cggctttgcc gatgtcgcca gcggaccatt cgtccggtcg tcgtaccacg
     3841 ccgacgaaat ggctcgcctg gaactgatgc ccggcgcgga attgcaattg tcgtcaggca
     3901 acggggcggg gtcctcctaa acaccaagca cccgatcgcg ggcggtgacc tcccactggc
     3961 cgacgacttc gccatcctcg acaacgtagg cccggcgatg aagtgcgaca gtcgggcaaa
     4021 cgtgcgtcgg gatcgcgaat aggggagtgc cgaccgggag gctctcggcg tgtgacgtct
     4081 caacgaccat gtgctcttcg ctgtgaacga cgggtcgggc atcgtcgagc gagaggaaac
     4141 gggcgcgggg cccgacgggg tcggaggcca cggccttgta accgagatcg aagcagaccc
     4201 gcccggcgct gggatgactg accacgcgtg tcaagagcaa ggccgccggg atgaatggca
     4261 ggtcgggata tcgcgtcagg tagctgttgt cgtgtagcac cgtcgttccg ggcgaacatt
     4321 cgaggcctgg tagatcgagc ttggcatgga tcggcaaagt cggcgtgccg ccgacgacca
     4381 gccgaggcac gggcaatcct cgtttcaaga gacgctctcg caacgccagc gtactttcca
     4441 ggcccgattg agcctcttgc cggcgtttgt cgagatcggt ctcgtggatg tggccgtcgt
     4501 aggcatgcaa cccatcggga accaggttgg gcaatcggtc gatcagggcg tacagctctg
     4561 cggcggcgtc gcccggttga atcccggtcc ggcccattcc gatctcgagg tcgaccaaga
     4621 ccggcacggg cgcgccgagc ccctcagcgg cagccgataa cgcccgggcc gcttcggaat
     4681 tgtcgaccgt ggcacgaaag gtcgtggaac gatacccgcg ggccagatag acgaaccgct
     4741 tcaggtttgg accggtcaac ggatacgcca gcagtacgtc ggttcctccc gcggccgcca
     4801 ccatctcggc ctcggcgatc gtcgcacatt tgtgcttgcg gatcccagat gactcgatca
     4861 agcggacgac gtctgccatt ttgtgggtct tgacgtgcgg ccgcagccta ccggcccctc
     4921 ctgccagaga aatcatctcg tgtaggttat gccgaatgag cctgggataa atcaaaagcg
     4981 aagggctgag aagttctgac gcgttattaa ccaggtgatg agggtccacc atggggatat
     5041 tggcctctga gtgagaaagc ggttatgttc ccgacagttt aactgaaaaa caggtcgcat
     5101 cgcgagcgga tcgtcggccg ccacagtgcg ttgatgcccg gcacgcagta atggtgcggt
     5161 gggtgacgcc gtttcatcca tggttgctca gggagccgga atgaccggac aagaattgag
     5221 gaggcgccgt ccttgctggc cgtcgatgtg gagttcgtcg agcaagcgac tgcggacggt
     5281 tcgcagcagt gattccacgg tttcgggacg aagatcggcc gcgacgatcg agccgccggc
     5341 gcgcttgtcg tgaccgcccg ccgagccgag tccgtttact acatggtgca gtgtctcacc
     5401 cgcgcgtccc ccaagcgtac ctgcacgcag ggacaatttc agcttgtcgt cgaacactcc
     5461 cgcggccagc gaccaatcca cctggtcgaa gcggataaag aagtcggcga tctcggcgat
     5521 gatatccggc tgtgacaccg cgccgcacca actgacgatc agcttgtcat aaatgaacgc
     5581 gttggcaagc gcgtgttgaa aagtggcgaa gtggctctgc ggcagcttgg gattgcggat
     5641 gcgggccagc aagtctttgt cggcgcgagg aaacagccag atcaaggccc cgtcgtccag
     5701 agagcttgcc tcgcgcggat aacccgtggt ctcggagtca atcccgtaga gcagggcggt
     5761 cgctagctga ggcgtcacga ccacgcgctg ttcgatcagg tagccggcaa tcatggtgct
     5821 ggtcgcgccc atatgggtgc ggatatcgct aaagagcacc ccctggagca ctccgccggt
     5881 ctcatgatgg tcgatgacca cttgcggcat ggccgcctca ctgtagcgcc ggccggtgaa
     5941 cggctgggtg tcgaccatga ccacggccgt ttcggagtcg gtagtgaccg tctcgactgg
     6001 aaccaagggg accggaatca gctcgaccat agcccggttc tcggaccggg caatcatgcc
     6061 gtcgacggtc aggatgactg gcctaccagg ctgggcggac tcgaccaggg ctttgatccc
     6121 cagcatgctt gccagcgagt ccggatcggg attaacgtgc gacaccacca cgacgcgacg
     6181 aaactgcgat agcaccgcca gcagtcggtc ggagcgggtg agccgggcaa attccggctc
     6241 ggatacagac tccgtactgt ggggatcggc cgtcatcgtc ggcttggtcc caggtgctcg
     6301 cgctgcgcac gggcaccaac tggtgtcgaa cgagccaacc ggttggatcg catgattcca
     6361 agagtgtacg ccaatcgagt catcgttggc cagagccagg cggccgcgtc gcgtcagact
     6421 cggaggccga ggggagggtc atatgtgggc tcactcgacc ttgaacagcg cgcgaatcag
     6481 tggccgaacc gcggcgcgca cgggcctggt cacgcgcgcg cttccgagca tagaacgact
     6541 cgatctcgtc gaccgtctcg tcgtcgatca atgggtcgcg ttcggcggac tcacccggct
     6601 catgcttccg tggggggcgc agcccgtact tggcttcgac atcctggatg acctggttaa
     6661 ccccgttgtc gaaccggact cgctcagcgg gtgtcagttc gacgatcccc gccgtccggg
     6721 gagccggccg cgccgcgaat gcaatcaaca atccgatccc cgccaaaagt actaacccat
     6781 tgagccggac cgccctgcgg tgaagctggt tgaatcgctc gcggccggcc gggccggcat
     6841 cgcgagcctg gttgatcgcc ggcgtgagcg agttgccccc gtaaagcatg cacaaaatgc
     6901 cggcgaggat cacgagagct tgcaccccga ccatcgggcc ccgaaattcg tgatagcaaa
     6961 gcggacccgc cacgaatgcc ggcaacgcga tcgcgcctgc aatcgccccc cagagatagt
     7021 agcgggggaa gagggcgcgg acgaacttgc cacccatctg ctccccgaga acgccgaaaa
     7081 tgatgggagc cacaccaaat gagaagaaca aaatgctgcc gacccacgcc gtcagcgcga
     7141 tgacgtacat cgagtcaaat agcgccagca aaacacgaga cgacatcgct tcacccaaat
     7201 ccgagcatgg ctacattgac caaccagatc gcgcacactg tgcgaacctg gtttgtccgt
     7261 tgctggaatt gtatccagaa ccggggggca tgtccggctc tcgacttaca cacattggcc
     7321 ggaacctcgc gctatgagaa ctcgacccca acgccagcga cgtttgcgac gatcaagcaa
     7381 cccggccggc cgttagcccg tcaccgcgtg gatgcttgcg cagcattgac cagttatccc
     7441 gaaaatcccc ttcaaccccc acaatcatcg acgaaggatc atacataatc gcgataccgt
     7501 acgcctcggt tgaactttca tcacgacaga gcttaatgcc gaaagtctag tcagagaatg
     7561 cacaggaacg gatgcgatcc actccaggat ggagaaatcg atgagaccgg cattactgct
     7621 attggtggcg ctacccgtct tatcggggtg cgccaatcgg cagttaagtt tcagcaccct
     7681 tcgtcagacc tccactctgg ccgatatcca ggagactcag gtcatcgaga atttcgcccg
     7741 gctcgcggcg aatcctgggg cgcttccatt tctctcgttg gcgaatagcg gatccgcgac
     7801 cgtcactgac ggcggctcgg gaagtctcag ttttttgggt gcgcacagat tgttcacgga
     7861 aagcactatt ggcgtcagcg cgaatcgtag tatcgcgctc aactggggat tggcgccttt
     7921 gaacaatccg gatcgattga aggccatgaa agccgcgtat ctctcggtac tcgatccatc
     7981 attggtcgat cccgccgaac agaccaagct caacgacgtg atcaaagccg acccatcgta
     8041 tgcgatcaat ccgggctgga tggaagtcgg cggcaaatgg gatgttccca aacacgcctg
     8101 gcgagttggt cgttgcggca agacttacgt ctgggtgatg cctgatcggg ccgaggactt
     8161 cagccgcttc gttttactga ctcttaatct tgcgtcattg caatcaacgg gtaaatcccc
     8221 gctggcacag gggcacgttg gcccgagcgc tgttgcgccc cccacgccga gcgccgccgc
     8281 gcctgccgcc gacccgttcc cccctcggct ttacgatcaa ccgccgagcg taaatcccgg
     8341 gctcttcttc gtgcctcgtg gcacagcgcc ctgaacgaag gtcggccgtc gaatcagcac
     8401 ttcttatttc gatttctgta ggcgggtata ctggtcgatc gttcgtccct gcgcggagtc
     8461 ttcgatgagt ttgtcgagcc gtttctgtct cgtctcttct tttttggcgc ttaccaccca
     8521 ccagctcacc actttgcggt aagacggaac ctgcgtttga aagaaatccc aggcagcttt
     8581 gttcgctcgc aacttctgtg cgtagggctc gggcatcgcg tcggttctct gctcgtacga
     8641 atagataccg gatcggttgt ccttccgctt ctcgaatgcc tcgaggccct tcgagtgcat
     8701 caagccttgg ttggtgagcg ctagggcgcg tctcacatta accgtgctcc atacactggt
     8761 cggcttacga ggagtaaatc gaatcttgta gctgatgtcg tcgatacttt tgcgaatccc
     8821 gtcaatccag ccgaagcaca gtgcttgatc gaccgactcc ggccatgtga tgctcgacct
     8881 gcccgaggtc ttcttgtaga agccgaccca gagttccggc gccctagcat gatttgcctc
     8941 gagccacttg cggaaatccg atgatgattt gaagaatttg atcgtcatca gaaaaaagtg
     9001 cgtcagaaag caaattaggg cacgttcgta acttcagttc cgcccgtgtt cccggtcgct
     9061 ctgttcttcc atcgccttct tcttggcagg gtattcgatg acgacgagct ctttcttgtc
     9121 gggatcgtag ccgtactcct ggtagtacgg caacatagga agctgaatgc cgtcggcctt
     9181 gcccttcttg atgatgactt ccatgaactc ttcgtagctc ttgggctttg agccattgag
     9241 cgcctcgtag atgtccatgt cgtacttgac cttcatcttg gcaatgttgg caacagaggt
     9301 gcggtacgca tcggcctgaa gggtgatgta gtcggtggcg tgaatcttgc cgtcggtgac
     9361 ttgaccgccc tgggcgagct cgggcttcaa gttcct
//
LOCUS       DATAQL010000010         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1069901, whole genome shotgun sequence.
ACCESSION   DATAQL010000010 DATAQL010000000
VERSION     DATAQL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069901"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..404)
                     /locus_tag="VHS97_00200"
     CDS             complement(162..404)
                     /locus_tag="VHS97_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3446644.1"
                     /translation="MPICDLGTGMWAAQGILAALYERERTGKGRLVECSLLETAIGFS
                     SWTLAQSLADHQEPTRQGSRHRQNAPYQRMRKRDGI"
     gene            1077..1325
                     /locus_tag="VHS97_00205"
     CDS             1077..1325
                     /locus_tag="VHS97_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446645.1"
                     /translation="MTIDDLMQQIKETERLIDVYRNANEVIVGTEDQIESRRGLINRP
                     TVTAAEIGDQIVNILERRLAAMRAKLQKPGAEYQGGNR"
BASE COUNT          351 a          595 c          579 g          376 t
ORIGIN      
        1 ggtggctttc gtcgcgagca ccgcgttcat cacggtctcc aatgcctcgc ggctctccat
       61 ccgtcgctgg ttcgtcgcaa aacgcgggtc ctcgcaccat ttcgggtgcc ccgaggcctc
      121 ggcacagcgc acccagatcg attggccggc ggctcccacc atcagatgcc gtccctcttc
      181 cgcatgcgct ggtagggcgc gttctggcga tggcgcgagc cctggcgggt cggctcctga
      241 tgatcggcaa gcgactgggc caacgtccac gagctgaagc cgatcgcggt ttcgagcagc
      301 gagcattcga cgagacgccc cttgccggtg cgctctcgct cgtagagggc ggcgaggatg
      361 ccctgcgctg cccacatacc ggtgccgaga tcgcagatcg gcaggccggc cgaggtcccc
      421 ctcgggctcg ccggtcacgt gcatgatccc gcccatgccc tgcgcgatcg atagcggttc
      481 ggtcacatct gcagacgcgg cggctcgggc attggcgccc gagctgacgt aatgtcttgg
      541 cgaaaggtcc gcgccggagc tcgtggtcgc gagcaccttg ctcgtcacgc ttaccgacgt
      601 atcggcaaag tcggcacgag gctttcgact tttacccgtc tagcaactgg cgtcagccag
      661 gagctttgct tgtcgccggt gctggctttg agcacaacaa ccttccacct ctcgacaagc
      721 tggtctgtaa tagtcgcgtc ctggagctaa gacgacaccg atacggcggg cagtgcgaca
      781 ctggcggttg cgagcagcgg tcttatcggc ctctcggtcg acgcggctta atttccggtc
      841 aggataggat caaagcacga attatggtgc gcccgatctg ttgtgtgagc ggcttgcgtc
      901 cgtcgcggta accagggtag agatcccggg aatcgaaccc gcggccgctt tggaaaaagc
      961 aaggctcccg ggtggagcgc ggtcctctgc tctgtgggtg tcggctacac tcgtcaacga
     1021 gccgggttta aacagcgcag ggcccgatcg ctgaggcgga caatggatac taagacatga
     1081 cgatcgatga tttgatgcaa cagatcaaag agaccgagcg cctcatcgac gtgtatcgca
     1141 atgccaatga ggtgatcgtc ggcaccgaag atcaaatcga gagtaggcgt gggctcataa
     1201 accgacccac cgtcacggcg gccgagattg gcgatcagat tgtcaatatt ctcgagcgcc
     1261 ggcttgcggc gatgcgggca aagctgcaaa agcctggtgc cgaataccaa ggaggaaacc
     1321 gctaatcgcc catgccgccg ggcggagggc agcctgcagg attgcgtttc ttcccgactc
     1381 ccgccgcttg gttcaaccgc acacgagtac cgccgccctt tccggcgtag gcacaacact
     1441 cggattccgg ctgacgggcg cgaccggacc gctcgctcac actccctcac tcgtcaagtg
     1501 tgagatgatc cggaacctac ggtgaatcgc gacaattcac ctaggtctgg gcaggcgccg
     1561 gtggttcatc tggcgttgtt gcaccagcgg tacccgtacc gggagcaaat ccaactatag
     1621 ggcagaatct gaccccaacc ggacatgctg ctgaccgtca attcgatcag cttgccggct
     1681 cgcctggatt ccgccgccag ggatgaaatg ggacgaggtc ttcacaccac accttaggct
     1741 aagtctgacc ggactcttgc cccgctcacc ccgacccctt taatccattc tttcgtcgcg
     1801 atttgccgct ggaggtcatc gctacgtggc tgcttgcgcc ttggcgattt ccgcagctgt
     1861 gtagcggtag gcacgaaaca cctggttggt ttgttcctgt g
//
LOCUS       DATAQL010000011         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4734000, whole genome shotgun sequence.
ACCESSION   DATAQL010000011 DATAQL010000000
VERSION     DATAQL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4734000"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..1570
                     /locus_tag="VHS97_00210"
     CDS             428..1570
                     /locus_tag="VHS97_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3446646.1"
                     /translation="MHKRLRRGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRAQC
                     SNNLKQIGLALHNYHTAIDRFPMGVSKNPLWGPGDTDGQWGYGRWTGWSAQALLLTYM
                     EQNPLYNAANFSVGPGAGGGMLGSWPNSTIYNTVINTYLCPSDPNADFDRSNSYFASI
                     GSTTYESPLNSSGMFAAFTSYGLRDCTDGSSNTIAFAESLTGQDNAGFGYGSQQNTAS
                     GTNYRGNVATTAKDPGTGGQGTTDDPKSYYVTGAQFLYASANSAAVLQGLQACAAGWN
                     QPNSTYSSMRGFCWADANGGWTYCNIIQTPNHDSVFSGGGCRFGSGGDGIDSAFSYAV
                     SSNHPGGANVLMTDGSVHFIKDSVSRLTWWGLGTRNGGEVISSDSY"
     gene            complement(1752..1898)
                     /locus_tag="VHS97_00215"
     CDS             complement(1752..1898)
                     /locus_tag="VHS97_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446647.1"
                     /translation="MTRRRRAQDRRFAATGLPLIAGVFDLKISIDLSMDALSAGSFLL
                     SPEL"
     gene            1970..3316
                     /locus_tag="VHS97_00220"
     CDS             1970..3316
                     /locus_tag="VHS97_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3446648.1"
                     /translation="MSLRVALGCNPTRSAVAALVLAALGATCAILVRPDRDPNRLWRS
                     ARSELRAGRFDRAESTMRRLLALRPATEDDWFTIAQLAMATGRTNEALDDLAHVPDSH
                     PLAAQARLWEGQLELRRQRAAAAESSLRRAIEIDASLVAARRELVYLYGVQRRCHELS
                     AEFATLAEVAPMSFDQVSLWCLIGIAPWDPREVQPILAAFVQADPFDRASRLALAEAY
                     GSLGQYEDVEAVLKHLPMTDPGARAILARIAFDRGDAPTVESLVANGPDDHPILQLYR
                     GQLALLRRDLPVAIGHFRKWTAADPHDRARLHMLGDALVKSGKLVEGELYLQAARDHE
                     VLYKLIDRASTAEGRQNLSLLKDLGAAYQRVGLIPEAKAWYKLALARDPADPGVQVAL
                     YHLGAAAARARINSTTEPGPSRDGGMPAVTSECRDAMRSKGRDLAPRNSQTHDDAG"
     gene            3347..>3822
                     /locus_tag="VHS97_00225"
     CDS             3347..>3822
                     /locus_tag="VHS97_00225"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX3446649.1"
                     /translation="MYALPIIALVSLLAVVPAGDEPVPPRVLIFSKTAGFRHDAIASG
                     LAAVRELATDGGFAVDATEDAALFTPENLARYRAVVFLNSSGEVFDDKQKAAFQGFIR
                     GGGGLAAVHQGVTTLDKWPWYVTLVGGVKFAGHPEPQQATVHCEIRDHAATKELPET"
BASE COUNT          733 a         1210 c         1177 g          702 t
ORIGIN      
        1 ccgcttgaaa caataattat gatgcgaaca tagcgtgagt ctacccgcca agcgacctga
       61 ccgaaagtta tagaaaaaag acccaaaatt tcatggaccc ggatattgca gcccgtatgc
      121 tcaagcctga cggtcgggcc aaagtccgcc gcgtgcttcc gaggatgaga aatccgctca
      181 ggtcgggggg gtaggacgta ggtcgcgaat cattcgcgac caggcgaggc gcgggttggt
      241 tgtgcaaccg tgattgccta agagggcgcg ccagccgggc ttgattccgt ttgcgaagag
      301 ggcaagtttc aggaatcaac cggacgtttc gacatcttcc atagcgtgcg gatactgccc
      361 ctgacggtct tgtcgattgc acggtctcca caaaatctga gtcatatctg aatcgcagga
      421 gatttccatg cacaaacgtt tacgtcgagg gttcacactc atcgagctct tggtggtgat
      481 cgcgatcatc gcagtcttga tcgctctctt gttacccgcg gtacaagcag cgcgcgaagc
      541 ggctcgccgc gcccaatgca gtaataacct caagcaaatc ggcctcgcac tgcacaacta
      601 ccacacggcg atcgaccgct tcccgatggg cgtctcgaag aaccctctct ggggacccgg
      661 tgacaccgac gggcagtggg ggtacggccg ctggaccggc tggagcgccc aggcgctctt
      721 gctgacctac atggagcaaa acccgcttta caacgcggcc aatttctccg tcggcccggg
      781 tgccggcgga gggatgcttg gaagctggcc caatagcacg atttataaca cggtgatcaa
      841 cacctatctc tgcccgtccg atcccaacgc ggatttcgat cgcagcaaca gctatttcgc
      901 gagcatcggt tcgaccacct acgagagccc tctcaacagc tcagggatgt ttgcggcctt
      961 tacttcctat ggtctacgcg actgcaccga cgggtcgtcc aacaccatcg ccttcgccga
     1021 gtcgctgacc ggccaggata acgccggctt cgggtatggc agtcagcaga atacagcgtc
     1081 tggcaccaat taccggggca acgtcgccac caccgccaaa gatccgggca ctggcgggca
     1141 ggggacgaca gacgacccaa agagctacta cgtcacggga gcccagtttc tctacgcctc
     1201 ggctaactcg gccgcggtac ttcaagggct gcaagcctgc gctgcggggt ggaaccagcc
     1261 gaattcgact tattcgtcga tgcgcggatt ctgctgggct gacgccaatg gcggctggac
     1321 atattgcaac atcatccaga cgccgaacca cgactcggtc ttctccggcg ggggttgcag
     1381 gttcgggtcc ggcggcgacg gcatcgacag cgcgttcagc tatgctgtct cgagcaatca
     1441 cccgggcggg gcgaatgtcc ttatgaccga cggcagcgtc catttcatca aggacagcgt
     1501 gagtcgtctc acttggtggg gccttggaac acgcaatggc ggcgaagtta tcagctccga
     1561 cagttactaa gaagctcaag aaccggccga ggggcgtggc ggcgagagcc tgccttcccc
     1621 tctgccgagg gagggtcgac gatgctattc gataagacca gaagacctgc cgtggcgctt
     1681 ttagggatcg ccatgctgac tggcgttctc tcgcttggcg ggtgctctga ctcgacgaca
     1741 acaagctcag gttacagctc cggggacaag agaaacgatc ccgcgctcaa ggcgtccatg
     1801 gaaagatcga tggagatttt caagtcgaag acgccggcga ttaagggaag tccggtggcg
     1861 gcgaagcggc ggtcttgagc acgtcgtcgt ctcgtcatcc aagtgcccct ggcaaggagg
     1921 acctgacggt tgcccgggcc ggggatggcg tgaccgagga ggtaagccga tgagtcttcg
     1981 tgtcgctctc ggttgcaacc cgaccaggtc ggcggttgcc gccctggtct tggccgccct
     2041 gggtgcgacg tgcgccatcc tcgttcgacc cgaccgcgat ccgaatcggc tctggcgttc
     2101 ggcgcgcagc gagctccgcg ccgggcggtt cgaccgtgcc gagtcaacga tgagacgcct
     2161 gctcgcgttg cgtccagcga cggaagacga ctggttcacg atcgcccagc tcgcaatggc
     2221 cacggggcgg acgaacgagg ccctcgacga cctcgcccat gtgcccgaca gtcacccatt
     2281 ggcagcacag gcccgcctct gggagggaca gctcgaattg cgccgtcagc gagcagcggc
     2341 ggccgaatcg tcgttacgac gagccatcga gatcgacgcg agcttggtcg cagcccgaag
     2401 agagcttgtc tatctctacg gcgtgcagag acgatgccat gaactgtccg ccgagttcgc
     2461 cactctcgcc gaagtggcac ccatgagctt tgaccaagtg tcactctggt gcctgatcgg
     2521 gatcgcaccc tgggatcctc gagaagttca gcccatcctc gcggcgttcg tgcaagccga
     2581 cccctttgac cgggcgtcgc ggctggcgct ggcggaagcg tacggcagtc tcggacagta
     2641 tgaggatgtt gaggcggtcc tcaagcactt accgatgacg gaccccggcg cccgggcgat
     2701 cctcgcccgc atcgccttcg accgcggtga tgccccgacc gtcgagtcgc tggtggcgaa
     2761 cggccctgat gaccacccga tcctccagct ttaccggggc cagttggctc ttctccgccg
     2821 cgatctcccc gtggccattg gccacttccg caagtggacg gccgccgacc cccacgacag
     2881 ggccaggctg cacatgctcg gcgatgcgct cgtcaagtcc gggaaactgg ttgagggcga
     2941 gctttacttg caggccgccc gcgaccacga ggttctttac aaactcatcg atcgagcgtc
     3001 aaccgcggag ggacgacaga atttgagcct gctcaaggac ctgggcgcgg cctatcagcg
     3061 cgtcggcctc atccccgaag ccaaggcttg gtacaaactc gctctcgctc gagaccctgc
     3121 cgacccgggc gttcaagtgg cgttgtacca cctcggcgcc gcggcggccc gggcacggat
     3181 caattcaaca accgagcccg ggccctcacg tgacggcgga atgcccgccg tgacatcgga
     3241 gtgccgggat gccatgcggt cgaaaggccg tgatctcgcg cccaggaatt cacagaccca
     3301 tgacgatgct ggttgaccga aaccgttgtc cgagcgagct agccctatgt acgccttgcc
     3361 gatcattgcc cttgtgtctc tgctcgccgt cgtcccagca ggcgacgagc cggtgccgcc
     3421 gcgagtgctt atcttctcga agaccgccgg ttttcgtcac gacgcgatcg cgtccggcct
     3481 ggcggccgtt cgcgagctgg cgaccgacgg gggcttcgcc gtcgacgcca cggaagacgc
     3541 tgcccttttt acgcccgaga acctcgcgcg ctaccgcgcc gtcgtcttcc tcaatagttc
     3601 gggcgaggtt ttcgacgaca aacagaaggc ggcgtttcag ggcttcatcc ggggcggcgg
     3661 cgggcttgcc gccgtgcatc aaggcgtcac gacactggac aagtggccgt ggtacgttac
     3721 cttggtcggc ggtgtgaagt tcgcgggaca tcctgagccc cagcaagcga ctgtccactg
     3781 cgagatccgc gaccacgccg ccaccaaaga gctgcctgag ac
//
LOCUS       DATAQL010000012         7498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3207490, whole genome shotgun sequence.
ACCESSION   DATAQL010000012 DATAQL010000000
VERSION     DATAQL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3207490"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VHS97_00230"
     CDS             complement(<1..396)
                     /locus_tag="VHS97_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HEX3446650.1"
                     /translation="MRKLIVCRTGIVVLIIAAIAGRAGRAADTPGRPVVQPADDARKE
                     PKAKEKPVPEPGMRVVAGPVEVERAYRLSLGPWSWQFSAPESIDLPDIVDRIRGLFGM
                     LLILGVAAYLSDNRRAISRRVVLWGLGLQW"
     gene            complement(409..1752)
                     /locus_tag="VHS97_00235"
     CDS             complement(409..1752)
                     /locus_tag="VHS97_00235"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX3446651.1"
                     /translation="MRAVDIIRKKRDGLALSPAEIAAMVEGIATGEVADYQWSALLMA
                     IVWRGMKSHETAALTDAMLRSGRIIELGHIPGPKIDKHSTGGVGDKTSLILAPIAAAA
                     GVYVPMVSGRGLGHTGGTLDKLESIPGFRVDLDLDRFQEVLGCCGLVMIGQTSEIAPA
                     DKFLYALRDATATVESIPLIAASIMSKKLAEGIDGLVLDVKTGGGAFMENLDDSIALA
                     EAMCAIGARLGKRMVALITRMDQPLGCAVGNAVEVAECIDCLKGGGPGDLVSLSIELA
                     AEMVFMAGKAESLNDARTVCRRTIADGTALERFRRLVQEQGGDARALDDPSLLPAPRK
                     RVVLKSSESGFVHSLAARPVGHATMLLGAGRARMDSPVDHAVGVILHKKVGDLVRIDE
                     PLCTLLVNAESRLEEAINLIRNAYTFAAEPSAPTPLLVERIATAAASSLEIGSQA"
     gene            complement(1803..2447)
                     /gene="upp"
                     /locus_tag="VHS97_00240"
     CDS             complement(1803..2447)
                     /gene="upp"
                     /locus_tag="VHS97_00240"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX3446652.1"
                     /translation="MSSVVVSSHPLVHHHIATLRDSRTSPAEFRRVVRMLAVLLAQEA
                     TADLPTCAREVTTPLGRAEARILKDVVGIVPILRAGLGMADGVIELLPDAEVWHIGLY
                     RDETTLRPREYYNKFPVRTRVTLALLVDPMLATGGSAVRACEIIKSAGVTRLKLLSLI
                     AAPEGIELLSRLMPDVEIHIGAVDSGLNEVGFIYPGLGDAGDRQFGTGVEPAEE"
     gene            2968..3930
                     /locus_tag="VHS97_00245"
     CDS             2968..3930
                     /locus_tag="VHS97_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3446653.1"
                     /translation="MAVQFAVLASGSRGNSTLVCGGGAGVLIDVGMGPRAIGERLDSV
                     GASWSRIAAVVLTHTHSDHVDTGAFAELSRRGVVLYCHEGHRAELAANAGFRKLEEAR
                     LIKHYDHRPFLTSTGQRLEPIELRHDGGPTFGFRIEASVGRRQRAVSIGYLADTGSWS
                     ERMAESLIDVDVLGVEFNHDVALQRSSPRPEFLIARNLSDDGHLSNRQGAELIQAVLA
                     RSGAGALRHVVLLHLSEQCNQPELAIQAAGDVIQAAGRRSIVHAARQSPAFPNLWVTP
                     RFTRPRDPTGAGAERTVTTTKKVSSSNGSSRCYTGLLFGDSEQD"
     gene            3954..4727
                     /locus_tag="VHS97_00250"
     CDS             3954..4727
                     /locus_tag="VHS97_00250"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX3446654.1"
                     /translation="MPHYFASDVHLRTDRPERDCRFHAWLNRLTPDDSVVIAGDLCDF
                     WMGARRAHDNPLDCESLRTLAEFRRRGGSLAIMPGNHDEWLCPFYHAELGATIIAEPH
                     DMTLHGLRVRVVHGHLLGARRVWKSWMETRAFYEAFSYVPGPIAQTLDRILQSRNLHG
                     LKADEERHMKVFRDYAARCRGTADLVVIGHVHQPADDALSDPRLVVLGGWQRRSSFLQ
                     IDEKGATLHIEHDCEQNLHHDAQPRQPSLAHNGGDLGKI"
     gene            4702..5352
                     /locus_tag="VHS97_00255"
     CDS             4702..5352
                     /locus_tag="VHS97_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX3446655.1"
                     /translation="MEEILARFDHHIHTLKHSPDSQIDPFHLVKRAREVGLDGVVITE
                     HDYQWESEELAELAARAAPLLVFSGAEISAREGHFLVYGLPTLERVPAGVTLAELLET
                     VRHHQAAIVAAHPFRWDQPFEAIVAEHGPAFDALELVSNNVPTETRSKTEALVRKHPM
                     ACTGSSDAHELDVVGCYFTEFDVPIDSIADFVTALRNRRGRPRHRPGADLASGPVT"
     gene            complement(5370..5723)
                     /locus_tag="VHS97_00260"
     CDS             complement(5370..5723)
                     /locus_tag="VHS97_00260"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3446656.1"
                     /translation="MTLRELARETTESWFGKSQLVFIYGLTGGGGNLISAILRYAAGS
                     NPRIHSGGGSVVIMGLVGLWAVAGLRSGTGTGKSLGRLMFIFIFMTALLGIALPQYID
                     NWGHASGALVGVTLG"
     gene            complement(5766..6347)
                     /locus_tag="VHS97_00265"
     CDS             complement(5766..6347)
                     /locus_tag="VHS97_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX3446657.1"
                     /translation="MSLSSESCAVAFKEWAGICDALITGRQSIVVRKGGISEAAGPGV
                     FVPEHSQFWLFPTWVHQAEQGLRPSAGEVPVTHRTVGDGSIPICALVRVDLIGQVERE
                     ETLSSLEEFHVFTAETITKRFHYRRPGLWIMGARVWRREPGFTLAPTPEQAGCKTWVT
                     LEQPLATTGLTPVLDDTEWAVTRERLRSILECN"
     gene            complement(6344..7342)
                     /locus_tag="VHS97_00270"
     CDS             complement(6344..7342)
                     /locus_tag="VHS97_00270"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX3446658.1"
                     /translation="MRRVAILGAGGMGTALALLLSKAVPDVRLWSRDPEHAAQFARTR
                     INERHLPGIRVPESLRITAAAAEAAAAAELIVAAVPTSYLRSTLESLAHEIPAHVPVL
                     SVVKGIEYGTFARPSEIIHKALGPRPLAVLSGPSHAEELARGLPASVVVSGSDLVLVN
                     QIRDILSHDVFRVYTSTDALGVELAGALKNILGIAAGICDGLGSGDNAKAALLTRGLV
                     EITRLGLDLGARHETFYGLAGVGDVVTTCYSPYGRNRSVGQRIGRGETLERVLGEMVN
                     VAEGVPTTRSVYSLAQKRGVEMPITAELYQILFEGKPPPSAVADLMVRMPKVEWQW"
BASE COUNT         1506 a         2401 c         2183 g         1408 t
ORIGIN      
        1 ccattgcaag cccagccccc agaggacgac cctgcgcgag atcgcccggc ggttgtccga
       61 cagataggcc gccaccccga ggatcaagag cattccgaaa agaccgcgaa tccgatcgac
      121 gatgtccggc aggtcaatgg attccggcgc ggagaattgc cacgaccatg ggcccagcga
      181 aagtcgatac gcgcgctcaa cttccaccgg cccggcgacc acccgcatgc ctggttctgg
      241 gaccggcttt tccttggcct tcggctcttt gcgggcatcg tcagctggct ggaccacggg
      301 tcgcccaggc gtgtcggccg cccggccggc acggcccgca atggcggcga tgatgagcac
      361 gacgattcca gttcgacaaa cgatcaactt gcgcattcaa aacgaccttc aggcttgcga
      421 tccgatctca agggagctgg ctgcggccgt agcgatgcgt tcgaccagca ggggcgtcgg
      481 ggcggatggc tccgcggcaa acgtgtacgc attccggata aggttgatgg cctcttccag
      541 ccgcgattcg gcattcacga gcaaggtaca gagaggctcg tcgatccgga ccaagtcgcc
      601 gacctttttg tgcaagatca cgccgacggc gtgatcgacg gggctatcca tccgcgcgcg
      661 gccggctccc aagagcatcg tcgcgtggcc taccggccgg gccgctagcg agtggacaaa
      721 acccgactca cttgatttca aaaccactcg tttccgcggc gcgggcaaga gtgacggatc
      781 gtccagcgca cgagcatcac ccccctgctc ttgtacgagc cgccggaacc gctcaagcgc
      841 tgttccgtcg gcgatggtgc gcctgcagac ggttcgggcg tcgttgagag actcggcttt
      901 gccggccatg aagaccatct cggccgccaa ctcgatcgac aggctgacca ggtcgcccgg
      961 cccgcctccc tttaaacagt caatacactc cgccacttcg acggcattgc cgacagcgca
     1021 ccccagcggt tggtccatcc gcgtgatcag cgcgaccatc cgcttgccga gccttgcacc
     1081 gatcgcgcac atggcctcgg ccagtgcgat cgaatcatcc aggttttcca tgaacgcgcc
     1141 gccgcccgtt ttgacgtcga gcaccagccc gtcgatcccc tccgccagct ttttcgacat
     1201 gatcgacgcg gcgatcaacg ggatcgattc gacggtcgct gtcgcgtcgc gtaacgcata
     1261 gagaaacttg tcggcagggg cgatctcgga cgtctgacca atcatgacga gtccgcagca
     1321 acccagaact tcttgaaagc ggtcgaggtc gaggtcaacg cgaaacccgg ggatcgactc
     1381 gagcttgtcg agcgttccac cggtatgacc caggcctcgt cccgacacca tgggcacata
     1441 cactcccgcc gccgccgcga tgggcgccag gatcaaggac gtcttgtctc caactccgcc
     1501 ggtgctgtgc ttgtcaattt tgggtcccgg gatgtggccc aattcaatga tcctccccga
     1561 gcgcagcatg gcatcggtaa gggcggctgt ttcatgcgat ttcatccccc gccagacgat
     1621 cgccatcaag agggccgacc actggtagtc ggcaacctcg ccagtcgcga tcccctcgac
     1681 catcgcggca atctccgcgg gagaaagagc cagcccatcg cgtttcttgc gaatgatgtc
     1741 aacggcccgc atgacggaca aaacctcgat tttccaggta agccttaaac tgcgcgtcaa
     1801 ctctactctt ccgcaggctc tacaccggtg ccgaattgcc ggtcgcccgc gtcccctaag
     1861 cccggataga taaacccaac ctcgttcaac cccgaatcga ctgcaccgat gtggatctcc
     1921 acatcgggca tcagccgact cagtaactct atcccctcgg gggccgcgat caacgagagc
     1981 agcttgagcc gagtcactcc ggccgatttg atgatctcgc atgcccgcac ggccgaacca
     2041 ccggtggcaa gcatgggatc gaccaaaagg gccagggtaa cccgcgttcg gaccggaaac
     2101 ttgttgtagt attcacgcgg ccgcagggtc gtctcatcgc ggtacaggcc gatatgccac
     2161 acctcggcat cgggtaagag ctcgatgaca ccgtcggcca tccccagccc ggcgcggaga
     2221 atcggcacga tccccacgac gtccttcaag atcctggctt ccgcgcgacc caacggcgtc
     2281 gtgacctcgc gggcacaggt cggtaaatcg gcggttgcct cctgcgcgag caacaccgcg
     2341 agcatgcgaa ccacccggcg aaactccgca ggcgatgtcc gggagtcgcg tagcgtagcg
     2401 atatgatggt gcacgagtgg atgcgacgaa acgacgacac tcgacacact ttcctcccga
     2461 ttcagaacgc agcgtcccgc ggtcccgatt tctgcagccg attgcccatt caatcgcacg
     2521 aatttccgga ccgagatccc ttttcctgaa taaaatcccc aaaaaattca cgtccagatc
     2581 ggattttcca gatagtggac tgacgttttt cgttgcgagt tcctaagtct agattctcgc
     2641 gatggatggg cgcggacgct gcgatcggac cgggtcaatg cgcggattga ggttcgtcat
     2701 cgccatcttc aaccgttgga agcagatatg gtacacgacc ggacgcccgt cgaaacgatg
     2761 gttctagatt ttcttggcga cgatcgagcc gggtagtaac gacggatcat cttgcccgaa
     2821 ttgaccggtc cattctacac tggcccagtt ccgagagtag acggatcgag gaccggcggc
     2881 catcacatga ggtgcccctc ctgtggccgt ccatgtcgct gacatgactc tcgactgatc
     2941 actgacgttg caggaggttt cgtcatcatg gctgtccaat tcgccgtgct tgccagcggg
     3001 tcacgaggga attcgacgtt ggtttgtggc ggaggcgccg gcgtgttgat cgatgtgggc
     3061 atgggcccaa gagcgatcgg agagcggctc gacagcgtgg gcgcaagctg gtcgcggatc
     3121 gcggccgtgg tcttgacgca tacccattcc gatcacgtcg acaccggagc tttcgccgaa
     3181 ctttcgcggc gaggcgtcgt gctctactgc cacgaagggc accgggcgga gctggccgcc
     3241 aacgcggggt tccgaaagct cgaagaggcc aggttaatca aacattacga tcatcgtccg
     3301 tttttgacct ccacgggcca gagactcgag ccgatcgagc tccggcacga cggtggtccg
     3361 actttcgggt tcagaatcga ggcatcggtg ggaaggcgcc agcgagcggt aagcatcggc
     3421 tatcttgccg acacgggctc ctggtcagag cgaatggccg agagcttgat cgatgtcgac
     3481 gtgcttggtg tcgagttcaa tcacgatgtg gcccttcaaa ggtcatcacc acgacccgag
     3541 ttcctgatag cgcggaattt gagcgacgat ggtcacctct caaaccggca gggggccgag
     3601 ctgatccagg ccgtgctggc gcgatcgggc gcgggggcgc tgcgccacgt tgtgttgctg
     3661 catttgagcg agcagtgcaa tcaacccgag ctggcgattc aggccgcggg agacgtgatc
     3721 caagcggccg ggcgccgctc gatcgtccat gccgcgcggc agagccctgc attccccaat
     3781 ctctgggtga ctccccgttt cacgcgaccg cgtgacccga caggcgccgg cgccgaaaga
     3841 acagtcacaa caaccaagaa agtctcatcg tccaacggca gttcacggtg ttacactggt
     3901 cttttgttcg gcgactcgga gcaagattga cggatcgcga ggactaagag gctgtgccgc
     3961 actacttcgc cagcgacgtt catctgagaa cggatcgacc cgagcgcgac tgccgtttcc
     4021 acgcatggct aaaccgcctt acgcccgacg attccgtggt gattgccgga gatctttgcg
     4081 atttctggat gggtgcccgc cgcgctcatg acaatccact ggattgcgag agcctgcgga
     4141 cgctggccga gttccggcgc agaggcggct cgctggcgat catgcccggc aatcacgacg
     4201 agtggctttg cccgttttac catgccgagt tgggggcaac catcatcgcc gagcctcacg
     4261 atatgacgct gcacggcctt cgtgtcagag tcgtccacgg ccatctcttg ggagcgcggc
     4321 gagtttggaa gtcgtggatg gaaacccgcg ctttctacga ggcgttttct tacgttcccg
     4381 ggccgattgc ccagacactc gatcgaatcc tccagtcgcg caaccttcac ggcctgaagg
     4441 ctgacgaaga acgccacatg aaagtctttc gcgactacgc ggcccgatgt cgaggcacgg
     4501 ccgatctggt ggtgatcggc catgttcatc aaccggccga cgacgctctc tccgacccac
     4561 gcctggtcgt cctggggggc tggcagcgcc gctccagctt tctgcaaatc gacgaaaaag
     4621 gggcgactct gcacatcgag cacgattgcg aacagaatct gcaccacgac gcacagccac
     4681 gccagccgtc cttggctcat aatggaggag atcttggcaa gatttgatca tcacatacac
     4741 actcttaagc attcgcccga cagtcaaata gacccttttc atttggtgaa gcgtgcccgg
     4801 gaggttggtt tggatggagt agtaatcacc gaacacgact atcagtggga atcggaagag
     4861 ctggcggagc tcgccgcgag agcggcgcca ctcttggttt tttcgggggc agagatctcg
     4921 gcgcgcgagg ggcatttcct ggtttacggc ctgccgacac tcgagcgcgt gcccgcgggg
     4981 gtcacactgg ccgagttact cgagacggtc cggcatcatc aagccgcgat cgtggcggct
     5041 cacccatttc gatgggatca accgtttgaa gcgatcgtcg ccgagcatgg gccggcgttc
     5101 gacgcgctcg agctggttag taacaatgtg cccaccgaaa cgcgctccaa aaccgaggct
     5161 ctagtgcgaa aacatccgat ggcttgcact ggctcaagcg acgcgcatga gctcgacgtc
     5221 gtcggctgct atttcacaga atttgacgtt ccgatcgatt cgatcgccga cttcgtgacg
     5281 gcgcttcgca accgccgcgg gcgcccacgg caccgccccg gagccgacct tgccagtgga
     5341 ccggtgactt aatcagcttg gttccaacgt cacccgagcg tgaccccaac caaagcccca
     5401 ctggcgtggc cccagttatc gatgtactgt ggcagggcaa ttcccaaaag tgctgtcatg
     5461 aatataaaaa taaacattag tcggcccagc gacttccccg ttccggttcc tgaacgcaat
     5521 ccggcgaccg cccagagtcc gaccagcccc atgatcacga ccgaaccccc tcccgaatgg
     5581 attcgcggat tcgagccagc ggcataccgc agaattgccg aaatcagatt gcccccgcca
     5641 ccggttaagc catagataaa gacaagttga gatttgccga accacgactc cgtggtttca
     5701 cgggcgagct cacgcaaggt catacacgcc cttcctgggt aaagatctga cggccaggcg
     5761 tgccgtcaat tgcattcgag aatcgatcgc agcctttccc tcgtgacggc ccattcggtg
     5821 tcgtcgagca cgggcgtaag tccggttgtt gcgagtggct gttcgagtgt aacccaagtc
     5881 ttacaaccag cctgttcggg agttggggcc agcgtgaaac ccggctcgcg gcgccagacg
     5941 cgcgcaccca tgatccagag acccggccgc cgatagtgaa accgctttgt aatcgtctca
     6001 gccgtgaaaa catggaattc ctctaaagac gacagcgttt cttctctctc gacctggccg
     6061 atcaggtcga cgcgaaccaa ggcgcaaatc gggatcgatc cgtcgcccac ggttcgatga
     6121 gtcacgggaa cctcgccagc cgagggccgc aacccttgct ctgcttggtg aacccaagtg
     6181 ggaaaaagcc agaattgaga gtgttccgga acgaatactc caggaccggc ggcttcgctg
     6241 atccctccct tgcggactac gatgctctgg cgcccagtga tcagggcatc gcagatgccc
     6301 gcccattcct tgaaggcgac agcacatgac tcggaagata agctcaccat tgccactcga
     6361 ccttcggcat tcgaaccatc aaatcggcca cagccgacgg cggcggtttc ccctcgaaca
     6421 gaatctggta tagctcggcc gtgatcggca tctcgactcc ccgcttctgc gcaagggaat
     6481 agacgctccg tgtcgtcggc accccttccg ccacattgac catctcgccg agtacacgct
     6541 cgagagtttc accgcggccg atccgttgtc caaccgagcg attgcggccg tacggactgt
     6601 agcaggtcgt gacgacgtcg cccacgccgg ccaggccgta aaacgtctca tgtcgtgccc
     6661 ccagatcgag cccgagccga gtaatctcca ccaagccgcg ggtcagcagg gcggccttgg
     6721 cgttatcgcc agatccgagc ccgtcgcaaa tgcccgccgc aattcccagg atgtttttca
     6781 gtgcccctgc cagctcgacc ccgagcgcat cggtgctggt gtagacacga aatacatcgt
     6841 gcgagaggat gtcgcggatc tggttgacca gcacgagatc cgagcccgac acgaccacgg
     6901 aagcaggcaa tccacgcgca agttcctcag catggctcgg cccgctgagc accgccaacg
     6961 ggcgtggccc gagtgcctta tggatgatct cgctgggccg cgcgaacgtt ccatattcga
     7021 tgccttttac aacactcagg acgggcacat gtgcagggat ttcgtgggca agcgactcca
     7081 gcgtcgaccg caggtatgac gtcggcaccg cggcgacgat cagctcggca gcggcggcgg
     7141 cctctgccgc ggcagccgtg atccgcagtg actccgggac cctgattccc ggcaagtgcc
     7201 gctcgttgat tcgtgtcctg gcgaactggg ctgcgtgctc gggatcgcgg gaccagagcc
     7261 gcacgtcggg caccgccttg ctcagcaaaa gcgcaagcgc cgtccccatt ccccccgcgc
     7321 ccagtatcgc aacgcgtctc atcgattcaa atccgccaaa gccttcgacc gaaaccggga
     7381 cacgacacct cactgccgac ggtacgctcg gtcgagcagg tcaatcggat gcaccacctc
     7441 gatcgacttg ccgcgttcct tgactttgcg cgcgatttga aggatgcagc ccacgttt
//
LOCUS       DATAQL010000013         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5192142, whole genome shotgun sequence.
ACCESSION   DATAQL010000013 DATAQL010000000
VERSION     DATAQL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5192142"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /locus_tag="VHS97_00275"
     CDS             complement(<1..2095)
                     /locus_tag="VHS97_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3446659.1"
                     /translation="MNRFSRRLGGLALAGLWAAVIPAQGADELQSKGTGPAYELIGKV
                     AVMHEGRVKPLDTVAREEIKQVYHRETMKLRDLNDEVEAILDPQGHAKKAAGGSRVAK
                     WGPVGAFIGWTMSPEYWDDQPFILVDYLPLRRKIVAETLTSRLNAIAEKSTTADDEKS
                     SLRKLAAGPETTAAELTAFVRGSKLPAEDKKTIAEVAVKLSEEHKWLTPRELEEAKIT
                     DKDHTHPFIEWASELQDQQRQFNANPQSAARLTELERRAVEAATRLSTYKAYSGERFQ
                     TTALVLIMPRPNNAKYLAATAKTIKEAREKGDPRDLPVFKLDELKAISTYWNNVPRDD
                     RRDPTEDAAFDERYTAWLRDNSVWVPLKVMLRSKPEELVEAGYPEPEVRAFLDAYREL
                     SDAESSSPGQVSEASAAKFLASSRALGEAVNPSKYPTTAKIERETHFNAMNPFWQAPY
                     AYGTAVTLLLLSLGFVSVAGKSSFPALMGSTIYRVGLASLAIGIALEIYGFYLRVRIS
                     SWAPVTNMYETVIWVALVAAVLSLVFEMIYRKVFTALAGSAVALLGTIVAANVPLLDP
                     SIRSLQPVLRDNFWLSTHVLCVVSSYAAFGLAWMLGLVSTIYYLTATYRRSPRFLELA
                     LPLIPGLLLLAGGGAGVAASYGWFGPEWSIGGNQASSSSQSAYGGDTLFYVFASMALL
                     GETISLASLLAIGGEV"
     gene            complement(2196..>4079)
                     /locus_tag="VHS97_00280"
     CDS             complement(2196..>4079)
                     /locus_tag="VHS97_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX3446660.1"
                     /translation="LGMNILCAALIRYPWKKRQTGFVVTHAGLLILLMGSYYSFRTAD
                     EGQVGMLEGDVRGELVRIDYPVIRVREVDPHTRQSSVEFDLPFRPGPFEWGTASSPKP
                     NLLERALSWVTFGVLGPSRENGEVLSKPGDPFKFVVKEHLPASAPAVDHVADPDGAPM
                     VRMGLQFKGPGMPKAQNAFRSEEDHWFGTERKFYRVVRSQPPALLAFSYVDRPELVDD
                     FLKPPSNPGTKGVARFRYRDRTGKERVFDWALDEQQGKAVGLPESELTVTLSEATEFP
                     TSTGRLDQILGDDSIPIAVFKIQSGKDEPITHMALANLPMVPNVIPPTEGSAKAPPRP
                     LASIHYMVAPTLDPKTNGRFGQIEVMAGPGKALYYRVFGRGKESKGELRAAGPLDLRK
                     PIVAFGGNANMPMTITFEVDQYLPEAIEKPIFQPVVLPKGQMGNGIAACRAEMTVGGQ
                     TKEFWLSRSENLDPPPPQTVAFRDSVYEISYDVDRKPLGFELKLDDFDMDFEPGTEQP
                     THFVSKVRLTDKSENIAEAPHTISMNNPLDHRGYTFYQSNYIRVRDRRTNQFTGQFQS
                     VFQVASNPGRPIIYGGCSLVVLGAFLQFYMRAGIFTDGGKKERERIAAKKKAELETKT
                     EDL"
BASE COUNT          794 a         1252 c         1235 g          798 t
ORIGIN      
        1 cgacttcgcc gccgatcgca agcagacttg caagcgaaat cgtctcaccg agcaaggcca
       61 tgcttgcgaa cacataaaag agagtgtcgc cgccataggc cgactgcgag ctggaagagg
      121 cttggttgcc gccgatgctc cattccggtc caaaccatcc atacgacgcc gccacgccag
      181 cacctccgcc ggccaggaga agaaggccgg gaatgagtgg aagggccagt tcgaggaacc
      241 gcggcgagcg gcggtaggtg gcggtcagat agtagatcgt tgatacgagg ccgagcatcc
      301 atgccagtcc aaaggccgca tagctcgaga ccacgcacag cacgtgggtc gacaaccaga
      361 agttgtcacg aaggacaggc tggaggcttc tgatgctcgg gtccaatagg ggaacattgg
      421 ccgcgacgat cgtgcccagc agcgcgaccg ccgaaccagc cagcgcggtg aacaccttgc
      481 gataaatcat ctcaaagacc agcgacaaga cggccgccac gagcgccacc cagatgaccg
      541 tctcgtacat gttggtgacg ggagcccagc tcgagatccg cacacgaagg taaaagccat
      601 agatctccag agcgattccg atcgccaggg aggcaaggcc gacgcgataa atcgtcgagc
      661 ccatgagagc cgggaacgat gatttcccgg ccacggaaac aaaccccagg ctcaagagca
      721 agagcgtgac ggcagttcca taggcgtacg gagcctgcca gaagggattc atcgcgttga
      781 agtgagtttc gcgttcgatc ttggcggtgg ttggatactt gctggggttg acggcctcgc
      841 ccaatgcacg cgaggaggcg agaaacttcg cggcagatgc ttcggacacc tgaccgggtg
      901 agctcgactc ggcatcggac agttcgcgat aggcgtccag gaacgcacga acctcaggtt
      961 cggggtaacc cgcctcgacc agctcttccg gtttggacct gagcatgacc ttgagcggca
     1021 cccagaccga gttatcgcgg agccaggccg tgtaccgttc atcgaatgcc gcgtcttcgg
     1081 tcgggtcacg acgatcgtcg cgcgggacgt tgttccagta agtcgagatg gccttgagct
     1141 catcgagctt gaagaccggc aggtcgcgcg gatctccctt ttctcgcgcc tccttgatcg
     1201 tcttggctgt cgcagcgagg tatttggcat tgttggggcg gggcatgatc agaaccaggg
     1261 cggtcgtctg aaaccgctct ccgctataag ccttgtaagt cgacagccgc gttgccgcct
     1321 ccacggcccg acgctcgagt tcggtgagcc gggcggccga ctggggattg gcgttaaatt
     1381 gccgctgctg atcctggagc tcgctggccc actcgatgaa cgggtgggtg tggtctttgt
     1441 cggtgatctt cgcctcttca agctcgcggg gagtgagcca tttatgctcc tcgctcagct
     1501 tgacggcaac ctcggcgatc gtcttcttgt cttcggccgg cagcttgctg ccgcgcacga
     1561 aggcagtcag ctctgcggcg gtcgtttccg ggccggcggc gagcttgcgc aacgaagact
     1621 tctcgtcatc ggcggtcgtc gacttctcgg cgatcgcatt gagcctggac gtgagcgttt
     1681 ccgccacgat cttccgcctc aaggggagat aatcgaccag gataaacggc tgatcgtccc
     1741 agtactcggg gctcatggtc cagccgatga aggcacctac tggcccccac ttggcgactc
     1801 gagaaccacc ggccgctttc ttggcatggc cctgcgggtc gaggatcgcc tccacctcgt
     1861 cgttcaagtc gcgcagcttc atggtctcgc ggtgatagac ctgcttgatt tcctcgcgag
     1921 caaccgtatc gagcggcttg acgcgccctt cgtgcatcac ggccaccttg ccgatcaact
     1981 cgtaggccgg gccggttccc ttcgattgga gctcatcggc tccctgcgcg ggaatcacgg
     2041 ccgcccacaa cccggccagt gccaacccac ctagtcgtct gctgaatcgg ttcatgttcg
     2101 cgtctcccag ggctcgtcac gcgtgacgtc aactcttgtt ttcaataggt ctgtatgtga
     2161 tcccggccag ccaaagcgct cgccggccgt tgttcctaga gatcttcggt tttcgtttcc
     2221 agctcggctt ttttcttggc cgcgatccgc tcacgctctt tcttgccacc gtcggtaaag
     2281 attcccgccc gcatgtaaaa ctgcaagaag gcacccagca ccacgagcga gcaaccaccg
     2341 tagataatcg gccggccagg attgctcgcg acctgaaaga ccgactgaaa ctgccctgtg
     2401 aactggttgg tgcgccggtc gcgcacgcgg atatagttcg actggtaaaa ggtgtagccg
     2461 cgatggtcca gcggattgtt catcgatatc gtatgcggag cctcggcgat gttctcggac
     2521 ttgtcggtca ggcggacctt gctgacgaag tgggtcggct gctcggtacc cggctcgaag
     2581 tccatgtcaa aatcgtcgag cttgagctcg aagcctagcg gcttgcgatc cacatcgtac
     2641 gagatctcgt aaacggaatc acggaaagcg acggtctgcg gcgggggtgg gtcgaggttc
     2701 tcagagcggc tgagccagaa ttccttggtc tggccgccga ccgtcatctc cgccctgcac
     2761 gctgcgatcc cgttgcccat ctgtcccttg ggcaacacga ccggctggaa gatcggcttt
     2821 tcgatcgcct ctggcaaata ctgatcgacc tcgaacgtga tcgtcatggg catattggcg
     2881 ttgccgccga aggcgacaat gggcttacga aggtcaagtg gtccagcggc gcggagttcg
     2941 cctttgctct ctttgccccg accgaaaact cggtaataga gggccttgcc cggcccggcc
     3001 atgacctcaa tctggccaaa gcggccattg gtcttgggat cgagggtggg agcgaccatg
     3061 tagtgaatcg acgccagtgg ccgcggcggc gcttttgccg acccttccgt cggcggaatc
     3121 acgttgggga ccatcggcag gttggcgagc gccatatggg tgatcggctc gtccttaccc
     3181 gactggatct tgaatacggc aatgggaatc gagtcatcac ccaggatctg gtccaaacgt
     3241 ccggtgctgg tcggaaattc ggtggcctcc gagagcgtga cggtcagctc gctctcgggc
     3301 aatccgacgg ccttgccttg ttgttcatca agcgcccagt cgaagacacg ctcttttccg
     3361 gtgcgatcgc gatagcggaa ccgagcgacc cctttggtcc ctggattcga cggcggtttg
     3421 aggaagtcgt cgaccagctc aggccgatcg acatacgaaa acgccagcag agccgggggc
     3481 tggctgcgaa cgaccctgta aaacttgcgc tcggtcccaa accagtgatc ttcttctgac
     3541 cggaaggcat tctgcgcctt gggcataccc ggccccttga actgcagccc catccggacc
     3601 atcggcgcac catccggatc ggcgacatgg tccaccgcgg gggccgacgc cggcaaatgc
     3661 tccttgacta cgaacttgaa cggatcgcct ggcttgctca agacctcgcc attttctcga
     3721 gacgggccga ggaccccgaa tgtaacccac gacagagccc gctccagcag attgggtttc
     3781 ggactcgacg cggtccccca ctcgaaaggg cccggacgaa acggtaggtc gaattcaacc
     3841 gacgactgac gggtgtgggg atcgacctcc cggacgcgga tcacgggata gtcgatgcga
     3901 accaattcac ctctcacgtc accctcgagc atcccaacct ggccctcgtc ggcggttcga
     3961 aagctgtagt acgagcccat gagcaggatc aagagcccgg cgtgcgtgac gacaaacccc
     4021 gtctgccgct tcttccacgg gtaccggatc aaggccgcgc acaggatgtt catccccag
//
LOCUS       DATAQL010000014         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1070260, whole genome shotgun sequence.
ACCESSION   DATAQL010000014 DATAQL010000000
VERSION     DATAQL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070260"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VHS97_00285"
     CDS             complement(<1..859)
                     /locus_tag="VHS97_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446661.1"
                     /translation="MVRPHKCILVTTAFFIWMTLVASWAHALDTADARLLSNPAITEG
                     KIAFVYADDIWVADADGRNAKKVTSHAGEEQNPYFSPDGRHIAFTASYDGNVDVYVIP
                     SEGGEPTRLTWHPGEDIVRGFTPEGKVLFSSQRAVFSGRFAQFYLVGITGGVPEPLPV
                     PTADKGAVSPDGKFLAYTPLGERFRQWKNYRGGTVSRIWILKLDDLSHEEIPKPADGC
                     NDTEPMWIGESIYFLSDRDGEFNLYSYDRHSKKVTRCTEHDTFPVGSASAGVGKVIYE
                     QAGWIHVFEP"
     gene            complement(957..1649)
                     /locus_tag="VHS97_00290"
     CDS             complement(957..1649)
                     /locus_tag="VHS97_00290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3446662.1"
                     /translation="MIAIGDIHGCSRALDALLGAIEPQADDVIVTLGDCVNRGPDSRG
                     VLERLIALERQCKLISLTGNHDEMLLQLLMDAKVGRFRTLSNWLRMGGDATVASYGAP
                     QGKIAWADLTRIPAEHTAFLERCRAYHETATHIFIHANYDPVLPMKEQPFELLRWESL
                     RDAIPGPHYSGKTVIAGHSSQKTGEILDLGYLVCIDTYCYGGGWLTALDVQSGEVWQT
                     NLQGELRSSGSG"
BASE COUNT          367 a          570 c          585 g          404 t
ORIGIN      
        1 cgggctcgaa aacatggatc cagcctgcct gctcgtagat taccttgccc actccggcgg
       61 aagcgctgcc caccgggaat gtgtcgtgct cggtgcaacg agtgaccttc ttggaatgac
      121 ggtcgtacga gtaaagattg aattcaccgt ctcgatctga caggaaatag atcgactcgc
      181 cgatccacat cggctcggtg tcgttgcacc cgtccgccgg cttgggtatt tcctcgtgcg
      241 acaggtcgtc gagtttgagg atccagatac gcgagacggt ccctccgcga taattcttcc
      301 actggcgaaa tcgttcgccg agcggcgtat aagccaggaa ttttccgtcg ggcgagacgg
      361 cccctttgtc cgcggtgggc accggcaagg gctccggaac gccccccgtg atgccgacca
      421 agtagaactg cgcgaatcgc cctgagaaga ccgcgcgttg cgagctgaac aggactttcc
      481 cttcgggtgt gaagccgcga acgatgtcct cgccggggtg ccaggtgagc cgcgtcggtt
      541 cgcccccttc ggaggggatg acgtaaacgt caacgttgcc gtcgtagctg gccgtgaagg
      601 caatgtgcct accatcaggc gagaagtacg ggttttgctc ctccccagcg tgtgacgtta
      661 cctttttcgc attacggccg tcagcatcgg caacccagat gtcgtcggca tagacaaacg
      721 cgatctttcc ctcggtaatc gccgggttgg acaaaagacg agcgtcggct gtatccagag
      781 cgtgtgccca gctcgcgacc aaggtcatcc agatgaagaa ggccgtcgtc actagaatac
      841 atttgtgcgg tcgcaccatg gctatttcct cacttctcgt cgggtcaatg tccggccgtg
      901 tgatacgttt gccgcgggaa ttgattatga atgactgatc gaccgcgagc gagaccctaa
      961 ccggatcctg atgaacgcag ctcgccttgc aaatttgtct gccacacctc gccgctttgc
     1021 acgtcgagcg ctgtcagcca gccgccgccg tagcagtaag tatcgataca gacgaggtag
     1081 cccagatcga ggatctcgcc ggtcttttgc gacgagtggc cggcgatgac agtcttgccg
     1141 gaatagtgag ggcccgggat ggcgtcacgc agcgactccc agcgcaaaag ctcgaatggc
     1201 tgttccttca tcggcagcac ggggtcgtaa ttggcatgaa tgaagatgtg cgtcgcggtt
     1261 tcgtggtagg cacgacagcg ttcgaggaac gcagtgtgct cggcggggat tcgcgtgaga
     1321 tcggcccagg ctattttgcc ctgcggagcc ccatacgacg cgacagtggc gtcacccccc
     1381 atgcgcagcc agttcgaaag cgtacggaag cgccccacct tggcgtccat gagcaactga
     1441 aggagcatct catcgtggtt gcccgtgagc gagatcaatt tgcattgtcg ttcgagcgca
     1501 atcaatcgct ctagcactcc ccggctgtcc ggaccgcggt tgacgcaatc gcccagggtc
     1561 acgattacgt cgtcagcctg cggctcgatt gccccaagca aggcgtccag agctcgcgaa
     1621 cagccgtgga tgtcgccgat ggcaatcatt cgagacctca tattctctcc gtcggtcgag
     1681 actcttgctg gggctcaatc agtcgaaaca gtataatcag ttccgggtga attcgtgcgg
     1741 aaatcgagga tttgcagcat gccgggatta gtggcaacga gccggagaca gcgatcgcgg
     1801 acggccctgt cgctgttgct tgcgggcctc gcgctcttcg gctgcgcggg gtattgctgg
     1861 tttcgcgccg gcggttatgc gctgttctcc accgctcgcg tgccggcgct cccacggtcc
     1921 gcggct
//
LOCUS       DATAQL010000015         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_459676, whole genome shotgun sequence.
ACCESSION   DATAQL010000015 DATAQL010000000
VERSION     DATAQL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459676"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VHS97_00295"
     CDS             complement(<1..364)
                     /locus_tag="VHS97_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446663.1"
                     /translation="MGATGKYGHWIVWSIAAMVLFLAIGLAAFLPWAGLTRGPVVWTR
                     HSANQETSAVAAYDQGDWKRAADLSRQSLKAKGDDLQLLRVYARASARLERDETAAAV
                     YNRIGTAQLEPEDSLLLGL"
     gene            610..3591
                     /locus_tag="VHS97_00300"
     CDS             610..3591
                     /locus_tag="VHS97_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX3446664.1"
                     /translation="MSTSRSRSINDLMMRIGRHWRRLGIAAVLLALGWVVFARLEVWN
                     KLGELRLAQQEIARGQLAAAQKRLASLAARPGALGGAADYWLGICEAIGNRPDAALRA
                     FARLPDGYVFEPIGAYHEAKSNLTQGKLHAAERRLEQVLAPDGPGLDQVRDLLGQIYQ
                     IEVRFDDAKVLLRASLVDAKDPIRVLKELSNLELERLPYSGLQAALEKAGQLAPEDER
                     VCLGKGRLAIEAGRWEEARQWLERCRATHADTPVWRGLLELARGSGRPDEALDAARQL
                     RTGQLDHGERLSLLAWLHEQRGDTQAESSALEQWLRRDPAATRALERLAELAQQAGKS
                     DRVTDLRRRKTEVERALQAYRHLLWREEPLGGTAERYELAELAEAAGRRHEARALYTW
                     TLTADPDHRAGREALARLDRLDAERQLELSSHYEPWPSSVVVARPDRPHWGPEPVGAL
                     SFTDDAEAAGLRFVYDNAETIFHQQPEPFGGGLALLDYDGDGWLDVYCVQGGPFTPAS
                     KLGAPSASSGDRLFHNRRDGHFDDVTDASGIGRFPRGHGHGVTVGDIDGDGHPDLFVT
                     RWRSYALYRNKGDGTFEDVTTRSGLGGDRDWPTSAALADFDGDGDLDLYVCHYAAWDL
                     ENPRLCRNTANSEYINCNPLDSEALPDHLFRNDEGSFVDVTTAAGIADRDGRGLGVIA
                     ADLDDDGRVDLFVANDSSANFLFRNLGGMRFEEVGHAAGVAGNASGAYQAGMGVAAGD
                     LDGDGLVDLAVTNFYGESTTFYRNLGGGNFTDATASVGLAVASRRLLGFGVALFDADN
                     NGRLDLASANGHVNDLRPNYPYLMPAQLLISGVDGRLTDYSDRAGAPWTVPRMGRGLA
                     VGDLDNDGRLDVLILSHKQPLAFLHNRTEARPFLTLRLEGRGSNRDAIGAKVAVTAAG
                     RRRVAQRVGGGSFQSASDLRLHFGMGNADRVESIEIVWPSGRISRFRDLKADNGYLLR
                     EGALEPSPLNGFPNVRVEAGN"
BASE COUNT          680 a         1159 c         1156 g          692 t
ORIGIN      
        1 taaggcccag gagaagggaa tcctcgggct cgagttgcgc ggtccctatc cggttataga
       61 ccgcagcggc cgtttcatcg cgttctagcc gggccgacgc ccgcgcgtag actcgcagca
      121 gctggaggtc gtctcccttc gccttcaacg actggcgcga caagtccgcg gctcgcttcc
      181 agtctccctg atcgtacgcc gccaccgcgg aagtctcttg gttcgcggag tgacgcgtcc
      241 atacgaccgg cccacgcgtc agcccggccc acggcagaaa cgctgccagt ccgattgcca
      301 aaaacaagac catagccgcg atcgaccata cgatccaatg tccatatttc ccggtcgcgc
      361 ccatgtctcg tgtcacgttt caccgcaagg tcgatcatcg cgaaaaggct caagttccaa
      421 ggaagtcttt atatcagatt cccgattgca gattccagaa ttttgacaaa aggagaatgc
      481 tatatatctc aacgagaata aatacaacgg catggactca tagaatcagt gcttcccggc
      541 tcatcgcttc cgggaggtta attcgtgcgt gatcttgact gctcctccac ggtcgaccac
      601 gattagacta tgagtactag ccgctctcgc agcatcaacg acctgatgat gcgaataggc
      661 cggcattggc gtcggcttgg catcgcggcg gtgctgttgg ccttgggctg ggttgtgttt
      721 gctcggctcg aggtgtggaa taagctgggc gagctgcgac tggcccagca ggagatcgcc
      781 cgcggacagt tggcggctgc tcaaaagcgg ctggcgtcgt tagcggctcg gccgggagcg
      841 ttgggaggtg cggctgatta ctggctgggg atctgcgagg caatcggcaa ccgccctgat
      901 gctgcgctcc gcgccttcgc tcggctgccc gacgggtatg tatttgagcc cattggcgcg
      961 taccatgaag cgaaatcgaa tctgacgcag ggaaagctgc acgccgccga gcgacgcctc
     1021 gagcaggtct tggcccccga tggtcccggc ctggaccagg tccgcgacct gcttggccaa
     1081 atctatcaga tcgaggtccg attcgatgac gccaaggtcc tgcttcgcgc cagtctcgtg
     1141 gatgcgaagg acccgatccg cgtcttgaaa gaactgagca acctcgagct ggagcgtctg
     1201 ccctatagcg gtctgcaagc ggccctggaa aaggccggcc agctggcgcc cgaggatgag
     1261 cgcgtctgcc tcggtaaggg ccggctggct attgaagccg gtcgctggga ggaggcgcga
     1321 caatggttag agcgctgcag ggcaacacat gcggatacgc ctgtatggag aggcttgctc
     1381 gagttggccc gcggatccgg ccgacccgac gaggccctcg atgcggcgcg gcagctacgc
     1441 accggacaac tcgaccacgg agagcggctt tccctcctcg cgtggttgca cgaacaacgg
     1501 ggcgatacgc aggcggagtc ttccgccctc gagcaatggc tccggcgtga ccctgcggcc
     1561 acgagagcgt tggaacgcct tgccgagctg gcccagcagg ccggcaagtc ggaccgagtg
     1621 accgatctca gacggcgcaa gaccgaggtc gagcgggccc tgcaggcgta ccggcactta
     1681 ctttggcggg aagagcctct cggcggaacg gccgagcggt acgagctggc tgaactggca
     1741 gaggccgccg gtcgccgcca cgaggcccgc gctctgtaca cttggacgct gacagccgat
     1801 ccggaccatc gggccggccg agaggccctc gcccgacttg atcgattgga tgccgaacgg
     1861 cagctcgaac tcagctcgca ctacgagcca tggccgagta gcgttgtggt ggcacgcccg
     1921 gatcgcccgc attgggggcc ggagcccgtc ggcgcgcttt ccttcaccga cgatgccgag
     1981 gccgccggcc tgcgattcgt ctacgacaac gccgagacaa tcttccacca acagcccgag
     2041 ccgttcggcg gcggcctcgc tctgctggac tatgacggcg acggctggct cgacgtctac
     2101 tgcgttcagg gcgggccatt cacaccggcc tccaagctcg gtgcgccatc ggccagctca
     2161 ggcgatcggt tatttcacaa tcggcgcgac ggccactttg acgacgtcac cgacgcttcc
     2221 gggatcggtc gcttcccccg cggacacggc catggtgtca cagtcggcga catcgacggt
     2281 gacggccacc cggacctgtt cgtcactcgc tggcggtcat atgcccttta ccgcaacaag
     2341 ggggatggaa ccttcgagga cgtgaccacc cgctccggcc tgggtggtga cagggattgg
     2401 ccgacatcgg cggccctggc tgacttcgat ggcgacggcg accttgacct ctacgtctgc
     2461 cactacgcgg cctgggatct cgaaaaccct cgactctgtc gcaacaccgc caatagtgaa
     2521 tacatcaact gtaacccact cgactccgaa gctcttcccg accacttgtt ccgaaatgac
     2581 gagggcagtt tcgttgatgt gacgacggcc gcggggatcg ccgaccgcga cggccgcggc
     2641 cttggcgtta ttgccgccga tctcgacgac gatggtagag tggacctttt cgtcgccaat
     2701 gactcatcgg ccaactttct attccgcaac ttgggcggca tgcgatttga ggaggtaggg
     2761 cacgctgccg gtgttgcggg caatgcctcg ggggcctatc aggccggcat gggtgtcgct
     2821 gcgggcgacc tcgacggcga tgggctagtc gaccttgccg tcaccaattt ctatggcgag
     2881 tcgaccacgt tttatcgcaa tctgggggga ggcaacttca ccgacgcgac agcctcggtg
     2941 ggcttggcag tcgccagccg gcggttgctg ggcttcgggg tcgccctttt cgacgccgac
     3001 aataacggtc ggctcgacct ggcctcagcc aatggccacg tgaacgacct gcggcccaat
     3061 tacccttacc tgatgcccgc gcaacttctg atcagcggtg tcgacggccg cctgaccgat
     3121 tactccgacc gggccggtgc cccttggacg gtcccgcgga tgggccgcgg cttggcggta
     3181 ggcgatctcg acaatgacgg tcggttggac gtgctgatcc tgtctcacaa acagcccttg
     3241 gcctttcttc ataaccggac agaagcgcgc ccgttcctga ctctccgact ggagggtcgc
     3301 ggctcgaatc gcgacgccat cggagcgaaa gtcgcggtca ccgcggcagg acggcggcgc
     3361 gtcgcccaac gagttggcgg cggcagcttc caatcggcat ccgacctacg tcttcatttc
     3421 ggtatgggca atgctgatcg ggtcgaatcg atcgagatcg tgtggccctc cggacgcatc
     3481 agccgttttc gcgacctgaa agccgacaac ggctatctgc tgagagaggg agctcttgag
     3541 ccgagcccac ttaatggttt tccaaacgtg cgagtcgagg ccgggaatta atgtgacagc
     3601 ccggacacac cgattccaac tgattggtgt aacaaacatt tgcacacttc ttggaggctg
     3661 agcgtcatgt ccgagcacaa agtcacg
//
LOCUS       DATAQL010000016         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_917730, whole genome shotgun sequence.
ACCESSION   DATAQL010000016 DATAQL010000000
VERSION     DATAQL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917730"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..662)
                     /locus_tag="VHS97_00305"
                     /pseudo
     CDS             complement(48..662)
                     /locus_tag="VHS97_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            complement(734..2680)
                     /locus_tag="VHS97_00310"
     CDS             complement(734..2680)
                     /locus_tag="VHS97_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446665.1"
                     /translation="MVDQSRKASLPWLLAGAFIIALLGLPCRGSDLFRDDFARYPSGW
                     LTRPLGQLNGAIQEYHYLTHRGVPLESWANAICHLDAWAVGDEDGTPYLEQHTVNDQA
                     EIMSPLFITGDPEWGDYTVGTEIKPLSLDNMAGVVFRYHTNRHYYRFALHGGKTARLA
                     VRLPLENEFRTAQWRGLGSVSFPYDTTRYYRLTVENAGAAIKAYIDGKLVLTADDGEL
                     IRGKVGVTANIPARFRDFRVAAPDEVKKGIAERIAQREAGLAQHRANNPRPKLWKSFN
                     TPQFGAGRNVRFGDLDGDGFPEMLIAQNIPRIADNFIQISCMTAVNFDGKVLWQLGRP
                     DPRNGLLTADTPFQVHDIDGDGRNEVVMVKDFQLQIREGATGRLRQSAPMPPALVDNR
                     LKPYALTNGDSLAFLDLRGHGKRQEVMIKDRYTHFWIYNNHLEPLWQGQGQTGHYPYP
                     FDIDGDGHDELAIGYAVWNHKGHQLWSRDRELRDHADGLAVGSFGSDPKAEPRVYAWG
                     SDEGFLVFDRRGTIMKHERIGHAQTASVGKFRPDLPGLQLICVNFWKNPGIITLFDAD
                     GNLLAQDEPIHSGSPMLPVNWTGDGREFVLLSGNTREGGMIDGELRRVVMFPEDGHPD
                     LTAYVSNVTGDARDEVILWDQERV"
     gene            2929..3576
                     /locus_tag="VHS97_00315"
     CDS             2929..3576
                     /locus_tag="VHS97_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446666.1"
                     /translation="MEKGKTDELGEVSEVLRRAAAGDEEALRALFARYHDRLKRMVHL
                     RLSRRLAGRVDDSDVLQEAFVEVARKLPLYVQEPKLPPFLWMRHLTALKLTEVHRRHL
                     GTQLRDADREVTLHRGGLPLADSVSLAAQLLGTLTTPSQAAIKAETRMLVQEALNGMD
                     PIDREVLALKHFEQLSTTEIAEVLGLSKAGAGSRYLRAIKRLRAILEQIPGFEAF"
     gene            3783..4157
                     /locus_tag="VHS97_00320"
     CDS             3783..4157
                     /locus_tag="VHS97_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3446667.1"
                     /translation="MARSVLTGRFEPTGEEKELPDEKPRIVPADETGPDTLSSSSLSL
                     PGVGARGGAKRQTHWEGVARIGHQVADALAYAHGQRIVDRDVKPSNLLLDNKGTVWVA
                     DCSVSLCVLAGNIHNSAITAAV"
BASE COUNT          797 a         1306 c         1348 g          840 t
ORIGIN      
        1 ccaagggccc aaaaccgatt caaggctgag ccgccgatcc ggtgaattca caggcaactc
       61 gcgggcgagg cgctcaacca tggcgacgat cgccggcccg gcaggctgat tctgaccgtg
      121 agggaagcag cgcagctcca gcttatcgaa ccgctccatt tcggtcggcg gctcagaaaa
      181 ggggctgcct cccccttgct catcagccag cggaggaacc agccgtaggc cgcctcgcgt
      241 ttcttccgct ggtagccatg gccgtcggca gagtcgacga ccaagccgat ccggtcgccg
      301 gtgccgtagg cttcgtagag aatgcggccg tattccgcca cggcacgcac cccctcgacc
      361 gggaaactct cgtcgcgctc tgcggcgatg atcaagagcg gcttcggcgc cgccatcgcc
      421 agcagttctt ggttattggc gtatcggatc agacccggga tgaagtggca gtgctcggcg
      481 gcttggtacc agtcgagtgg ccggcagaca tggatctgct cgccgaagtc actggtgccg
      541 accactggga cggccgccgc gatccggtcg tcgagggccg cggtgatcca cgtgttgtaa
      601 ccgcccccgg aggccccggt gatcccgatg cgctttgagt ccatctcttt tcgggacacg
      661 aggtattcaa gcgcacaacg cgtctcttac tcggcgatcc cttgttgagc aacgccgacc
      721 aagagcgcct cggtcagacg cgttcctgat cccaaaggat gacttcgtcg cgagcgtcgc
      781 cggtgacgtt ggacacgtag gctgtcaggt caggatggcc gtcctcgggg aacatcacga
      841 ctcgacggag ctcgccgtcg atcatgcccc cctcgcgggt attcccggag agcaacacga
      901 attcccgacc atcgccggtc cagttaaccg gcagcatcgg gctaccggag tgtataggtt
      961 cgtcctgggc caacaaattg ccgtcggcgt cgaacagcgt gatgatcccg ggattcttcc
     1021 agaagttcac gcagatgagc tggagcccgg gaaggtctgg cctgaacttg ccgacgctgg
     1081 cggtctgcgc atgcccgatg cgctcatgtt tcatgattgt ccctcggcgg tcgaacacga
     1141 gaaacccctc atcacttccc caagcataga cgcgcggctc agccttcggg tcggagccaa
     1201 agcttccaac cgccaagccg tcggcatggt cgcgcaattc gcgatcgcgg ctccagagct
     1261 gatgaccttt atggttccag accgcgtagc ctatcgcgag ttcgtcatgg ccgtcgccat
     1321 cgatgtcgaa tggataggga taatggcccg tctggccctg gccctgccaa agcggctcaa
     1381 gatggttgtt gtagatccaa aaatgggtat atcggtcttt gatcatgacc tcctggcgct
     1441 ttccgtgccc tcttagatcc aggaaagcca gtgagtcgcc attggtcagg gcgtagggct
     1501 tcaggcgatt gtcgaccagg gccggcggca tcggtgcaga ttgccgcagc cggcctgtag
     1561 ccccctcgcg gatctggagc tggaagtcct tgaccatcac gacttcgttg cggccatcgc
     1621 cgtcaatgtc gtggacctga aacggggtgt ctgcggtcaa tagtccattg cgcgggtcgg
     1681 ggcggcccag ttgccagagc accttgccgt cgaaattcac cgcggtcata caactgattt
     1741 ggatgaagtt gtcagcaatc cggggaatgt tctgggcgat gagcatctcg ggaaagccgt
     1801 cgccatcgag gtcgccgaat cggacattac gcccggcccc gaattgcggc gtgttgaacg
     1861 atttccagag cttgggccgc gggttattcg cgcggtgctg ggccagccca gcctctcgct
     1921 gggcgatccg ttcggcaatc cctttcttga cctcatccgg cgcggccaca cggaaatccc
     1981 gaaaccgtgc cgggatgttg gcggtgacgc ccactttgcc gcggatgagc tcgccgtcgt
     2041 cagccgtcag gacgagtttc ccatcgatat aagccttgat ggcggctccc gcgttttcga
     2101 ctgtcaggcg gtagtaacgc gtcgtgtcgt acgggaagga aacgctgccg agaccacgcc
     2161 attgcgccgt gcggaattca ttctcgagtg ggagacgcac ggccaggcgg gccgtcttgc
     2221 cgccgtgaag cgcgaagcga tagtagtggc gattggtgtg gtagcggaag acgactcccg
     2281 ccatgttgtc gagcgagagc ggctttatct cggttccgac tgtgtaatcc ccccattccg
     2341 ggtcaccggt gatgaagagc ggactcatga tttcggcctg gtcgttcacc gtgtgttgtt
     2401 ccagatatgg cgtgccgtct tcgtcgccga ctgcccaggc atcgaggtgg cagatcgcgt
     2461 tggcccagga ttccagggga acgccccggt gcgtcaaata gtggtattcc tggatcgcgc
     2521 cattcaactg accgagaggc ctggtcagcc atccactcgg gtaccgcgcg aagtcgtccc
     2581 ggaacagatc gctaccccgg cagggcaaac ccaacaacgc gatgatgaaa gccccagcca
     2641 ggagccacgg gagacttgct ttcctcgact ggtcgaccat gagcggcacc tcgcaatttc
     2701 gcggctgtgg atcgtctcgt gttgtcgcac ctttcgtacc ggccgagtaa accacgtgat
     2761 gccaaacggg aaaagaggct tcctctggcg gaggtgttgc gccgtcgagg atctgagtgt
     2821 gacttggaga atgacctact ttttggtcga acgctgtcga gtggaaaaag gttctggaaa
     2881 aacggcagtc tatggagacg atcagccgcg gattgcactt tataaggtat ggagaaagga
     2941 aagaccgacg agctgggaga ggtttccgag gttctgcggc gcgccgccgc gggcgatgaa
     3001 gaggcgctgc gcgcgttgtt cgctcgctat cacgaccgac tcaaacgcat ggtacacctg
     3061 cggctcagcc gccggctggc gggccgggtc gacgattcgg atgttcttca ggaagcgttc
     3121 gtcgaagtgg cgcgcaagct gcccttgtac gtccaggagc ccaagctgcc gccctttctg
     3181 tggatgcgac acctaaccgc cctcaaactg actgaggtcc accgccgtca cttgggtacc
     3241 cagcttcgtg acgccgaccg cgaggtcacg ctgcatcgtg gcggattgcc attggccgat
     3301 tcggtctcgc tggcagctca gcttttgggg acgttgacga ctccttcgca agccgcgatc
     3361 aaggccgaga cgcggatgct ggtgcaagag gcgctgaacg ggatggaccc gatcgaccgc
     3421 gaggtcttgg ccctcaagca cttcgaacag ctcagcacga cggagatcgc cgaagtcctg
     3481 ggcctctcca aggccggcgc cggcagccgt tatttgaggg cgatcaaacg cctccgcgcg
     3541 attctggaac agattcccgg cttcgaggcg ttttgagcct cgttttgtgc gttctcgaac
     3601 acgaggatga cgatggcgac gtctgaggcc caatcgcgct ctggcctggt cctggagctg
     3661 gccgaggagt tcctcgactg ctatcgcgcc ggctagcggc ccgcgctcgc ccacggcgga
     3721 cggggagggc gaaagtacgg ccggattatg catcgattca ccgcgatgca tccgacgccg
     3781 acgtggcgcg ctcggtcttg accggccggt tcgagccgac gggcgaggaa aaagaactcc
     3841 cggacgagaa gccccggatc gtacctgcgg atgagaccgg tccggacacc ctttcctcgt
     3901 cgtcattatc gctgccgggc gtcggggctc gcggcggagc gaaacgccag acccactggg
     3961 agggagtggc gcggataggt caccaggtgg ccgatgcgct ggcttacgct catggccagc
     4021 ggatcgtgga ccgcgacgtc aagccatcga accttttgct ggacaacaag gggaccgtct
     4081 gggtcgcgga ctgttccgtt tctctttgcg ttctcgcggg caacattcat aattcagcaa
     4141 taaccgccgc cgtgtgactg gagaccaacg ccatgtccga tgcaccacgc aatccaccat
     4201 tcaacaactc accgatccgt ttggcggtgt gcgtttcggg cgagggaacg accctccaaa
     4261 acctgatcga ccagattcga ttgcgtcgcc t
//
LOCUS       DATAQL010000017         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1223184, whole genome shotgun sequence.
ACCESSION   DATAQL010000017 DATAQL010000000
VERSION     DATAQL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223184"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2296)
                     /locus_tag="VHS97_00325"
     CDS             complement(65..2296)
                     /locus_tag="VHS97_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3446668.1"
                     /translation="MRPVSSCLPFALGVIAFGVGSPSAPPAPKLVLQAPPAASVNSVA
                     VSPDGAFVASAGEGGVRLYDAKSGVLVRAYGDVGDRSVAFSPDGRSLTAGGFHMDMLV
                     GIYDVRTGKRRRTLAGHKEWETDACTISPDGTLIASTGTDKQILVWDLATGELRNRLA
                     DQPFRVTTLAFSPDSTTLAGGGDKTIRIWDLRSGQLRRTLAGHGDWVCTIAFSPDGTT
                     IASGSCDWSFHRGHDWPRPEWKGPEKFEWRLWDALSGKLKRSMTESGQLLSLVFAPDG
                     KSLACGIGKDVRIYDFLSEAPGRVVTSHHYGVTSVAFTPDGAAIISGSHDHTIKRTSL
                     ATGRVEWHAPGSSEQVNSVALSEDGSLLATGSSDGRFALGVRQAGTKGIGPGAVRLWD
                     ARTGRLIRRLGDPADQIMAVALAPSGQVVLAGGGSPGGKGVLSVWNAATGAPLWSVGD
                     HAAEVLAVAFAPDGSSLASGSADGQIKIRDPRTGSVMRTLAGHVRGATSLAFSADGVA
                     LACGRGDGTTSLWEIQTGQQIRTFRPARSQAGVIKDDRPITSIALSRDGETLATCTAG
                     VNQTFAEPVRIWDVRTGELKRQFSEPTIAGRPMALSPDGTVLATGGKSVRLWDARTGK
                     PLRDLMGHLKRTQSIAFSADGRLLFSGGSYGTTNAWEVATGRHLVTLFAFPEQRDGAV
                     EEEWLAYHPDGYYNGSSDVDRFLAWRVGSELLTPASLGPQLHRPERLESALKLDLVKA
                     SLP"
     gene            complement(2389..>2728)
                     /locus_tag="VHS97_00330"
     CDS             complement(2389..>2728)
                     /locus_tag="VHS97_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX3446669.1"
                     /translation="GAALAIALTTGVAFAVVAERERRSRAEHLIEEVAALYRKADWFR
                     DQARRIPPDQLDTWERALAHVRRTAEIVGAGAIDKVTRVSVDRLINELRDEEKRIRER
                     VREQLNRKPE"
BASE COUNT          482 a          906 c          862 g          478 t
ORIGIN      
        1 cgctctatcc gccaagtaga agttgccgca agcccaacgg cggctaattg tgaataggga
       61 ccacttaggg caagctggct ttcacgaggt cgagtttcag ggcagactcc agccgttctg
      121 ggcggtggag ctgcggaccc agggatgccg gcgtcagcag ctcactgccg actcgccaag
      181 cgaggaaccg atcgacgtcg gacgagccgt tgtagtatcc gtccggatga taggccagcc
      241 attcttcctc gaccgcgccg tcgcgttgtt caggaaacgc aaacaacgtg accaggtgtc
      301 gcccggtcgc gacctcccag gcgttcgttg ttccataact gccaccgctg aacaggagcc
      361 gcccgtccgc tgagaacgca atcgactgtg tcctcttgag atgccccatg agatcgcgga
      421 gcggcttgcc ggttcgcgcg tcccagagcc tgacggactt gccgcccgtc gcaagaacgg
      481 tcccgtcggg cgagagggcc atcggcctgc ccgcgatcgt aggctcggag aactggcgct
      541 tcaactcgcc cgttcgaacg tcccagattc tcaccggctc ggcgaatgtc tggttcacgc
      601 cggcggtgca cgtcgcaagt gtctctccgt cgcggctgag cgcgatggac gtgatcggcc
      661 ggtcgtcctt gatcaccccg gcctgcgaac gcgcgggtcg gaaggtgcgg atctgttgtc
      721 ctgtttgaat ctcccagagg gaagtggtcc cgtcgccgcg gccacaggcg agggccacgc
      781 cgtcggccga gaaggcaagc gatgtggcgc cccgcacatg ccccgccagg gtccgcatta
      841 cggagcccgt ccgggggtca cggatcttga tctgtccgtc agcactaccg ctggcgagtg
      901 aggacccgtc cggcgcgaag gcgactgcga gcacctccgc cgcgtgatct cccacagacc
      961 agagaggcgc cccggtcgcg gcattccaga cgctgaggac ccctttgcca ccgggacttc
     1021 cgccgcccgc aagaacgacc tgtccgcttg gcgcgagcgc cactgccata atttgatcgg
     1081 ctggatctcc cagccgtcgg atcaagcggc cggttcgcgc gtcccatagc cggactgccc
     1141 caggcccgat cccctttgtc ccagcctggc gcacgcccaa ggcgaaacga ccgtcgctgc
     1201 tgcctgtagc caaaagcgag ccgtcctccg agagtgcgac cgaatttacc tgctcagagg
     1261 agccgggtgc gtgccattcg accctgccgg tcgcgaggct cgtgcgcttg atcgtatggt
     1321 cgtggctgcc gctgatgatc gcggcaccgt cgggcgtgaa agcgacggaa gtgacgccat
     1381 aatgatggct cgtcacgacc cggccgggag cctcggacaa gaaatcgtaa atccgaacat
     1441 cctttccaat tccacaggcg agggacttgc cgtcgggcgc gaagacgagc gacaggagtt
     1501 gccccgactc ggtcatggac cgcttgagtt tgcccgagag cgcatcccaa agcctccact
     1561 cgaacttctc tgggcccttc cattccggcc gaggccaatc atgaccacga tgaaacgacc
     1621 aatcgcaact cccactggcg attgtcgtcc catccggcga aaacgcgatg gtgcaaaccc
     1681 agtcaccatg gcccgcgagg gtccgacgaa gctgacctga cctcaagtcc caaatccgaa
     1741 ttgtcttgtc gccgccgccc gcaagtgtgg tgctatccgg cgaaaaggcg agggtggtca
     1801 cgcggaacgg ttggtcggcg agtcggttcc ggagctcccc ggttgcgagg tcccacacca
     1861 ggatctgctt atccgttcca gtcgaggcga ttagcgtgcc gtcgggagag attgtgcaag
     1921 cgtccgtttc ccactccttg tgtccggcga gagtgcgccg gcgcttgccc gtgcgaacgt
     1981 cgtagattcc gaccagcata tccatgtgga agcccccggc cgtcaaactc cgtccgtctg
     2041 gcgaaaaggc gaccgagcga tccccaacat ctccgtacgc acgcaccaga acgccggact
     2101 tcgcatcata aaggcggacc ccgccctcgc ccgcgctggc gacgaacgcc ccgtcgggcg
     2161 agacagccac tgaattcacc gacgccgcgg gcggcgcctg gaggacgagc ttcggtgccg
     2221 gaggcgcgct aggcgagccg acgccgaatg ctatcacgcc gagggcgaat ggcagacaag
     2281 agcttacggg gcgcatgagt ttgctcctga tctcgcggaa taggtcgata cgtttaggtc
     2341 tcgggttgcg gttggacgtg tcctcttgca aacgtctagt tcggcccgtc actcgggttt
     2401 ccgattcaat tgctcacgga cgcgctcgcg gatgcgcttc tcttcgtccc tgagttcgtt
     2461 gatgagcctg tcgacgctga ctcgcgtcac cttatcgatc gcaccggcgc cgacgatctc
     2521 ggcggtccgc cggacgtggg cgagcgcccg ttcccaggtg tcaagctgat cgggtgggat
     2581 gcgccgggcc tggtcacgga accagtccgc cttgcggtag agcgccgcca cctcctcgat
     2641 caagtgctcg gcgcgagacc ggcgttcgcg ctcggcgacg acggcgaacg cgacgccggt
     2701 cgtgagagca atggcgagcg cggcgccg
//
LOCUS       DATAQL010000018         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_765180, whole genome shotgun sequence.
ACCESSION   DATAQL010000018 DATAQL010000000
VERSION     DATAQL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765180"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..2417)
                     /locus_tag="VHS97_00335"
     CDS             complement(189..2417)
                     /locus_tag="VHS97_00335"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX3446670.1"
                     /translation="MDAIQPYKGDAPPPSTGRPPTSASLQFINPAAQKTTSDYFKAVR
                     HRIWMVLAIAVPFAIVSSIMVLRLPPVYVVKAEIEINPPQLDPTLSALLTHEIGRSDP
                     AAHASYIPNREARLRSKELAQRVVSNDSIVPAVSQFADPALELFKSLSVLQVKKNGNS
                     FIVTLEGGDPALTKRLLEILLEEFKKQAELEQGDKLRATQVYAEENLKNLKTALTALE
                     EEISKAMKTTRTVGPGGRNILEDEYINLGTMMSQKQLQINQLHQQMMISQMFPKFELD
                     GAAGGREARLAQLRADKRKYERGLDHMKRTMRNFNHDPAAEEWAKSLEAILDEIAELS
                     AFKPRETANYTDMFIEQYRNELDADKEQHDKLLAKLQEMMPDHQRVLTLLRDREDKAK
                     QISGLEQRLLEFGIVQQQLVHSDCVRIPASVVEPTVPMKPNRPMLIGFGLFASIGLGV
                     GLVCLLEYIDHSVKVPEHVTHGLTLPLLGVVPRIRRSALTHRGGHLWTPGTPDSIEAD
                     AYRNVRASLLGIGDKRGSIVTLLVTSAKAGEGKSTTALNLAATCARAGERTLLMDVDF
                     RRPSLADVFIEPESTSTVHGLVDVLRGDMPWQRTVRHTEIPNLDFIPTGDTRDIPIEI
                     LGTLELRQLLIALAHHYDRVILDGPAVLGLADCRVLGRIVDASLLVVRSGSHHLTTLH
                     RAKAMLEQSHVAIAGVVFNGLTDDMDNWSSYGYDPLPLGGGKPRSMASDADRLALATS
                     DV"
     gene            complement(2444..>2769)
                     /locus_tag="VHS97_00340"
     CDS             complement(2444..>2769)
                     /locus_tag="VHS97_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446671.1"
                     /translation="PSDSVLAADIVFRYLQRLSQALVDRLERLESSASSMTKTTVLGT
                     VPDPELGTSRGQGSALAALGLLGTILARVVDRNTPKRAAKAHIEAPNRAAADALATPA
                     SVSRG"
BASE COUNT          492 a          851 c          859 g          567 t
ORIGIN      
        1 ggatcatgac gagcacggca accgccggcc taaaccacca gatcgcaccg ccgaaacaca
       61 ccgcgctacc gaatacgagc gcgaccacgg ccacgctttg taagcggtca gtcgccagca
      121 ataatttcag ccgcagattc acgttgagcc tctcaagaaa tcgctccgtg gctcaggccg
      181 gcggccggtc aaacatcgct cgtcgccagc gccagccggt cggcgtcgct cgccattgac
      241 ctcggcttgc cgccgcccag aggaagcggg tcgtatccgt agctcgacca gttgtccatg
      301 tcatcggtca gtccgttaaa gacaacgccc gcgatggcca cgtgcgattg ttcgagcatg
      361 gccttggccc ggtggagggt cgtcaggtga tgcgagcccg agcgaaccac cagaagcgat
      421 gcgtcgacga tccgacccaa gacccggcaa tcggccaggc ccagcacggc cggaccgtcg
      481 aggatcaccc ggtcgtaatg gtgtgccagt gcgatcagca attgccgcag ctcgagcgtg
      541 cccaggatct cgatcgggat gtcgcgcgtg tctccggtcg gaatgaagtc gaggttggga
      601 atctcggtat ggcgaacggt tcgttgccac ggcatatcgc cccgcaacac atcgacgagc
      661 ccgtggaccg tggacgtgga ttcaggctcg atgaagacgt cggccaggct gggccggcgg
      721 aagtcgacgt ccatgagcaa cgtccgttcg cccgcccggg cacacgtggc ggccaggttg
      781 agcgcagtcg tgcttttacc ctcacccgcc ttggcgcttg tcaccagcaa ggtcacgatc
      841 gacccgcgct tgtcgccgat acccaaaaga ctggcgcgga cgttgcgata ggcatcggcc
      901 tcgattgaat cgggtgtacc gggcgtccag aggtgccccc cgcgatgggt cagtgcgctc
      961 cggcgaatcc gcggcaccac acccaatagt ggcaaggtga gtccgtgcgt cacatgctcg
     1021 gggactttca ccgagtggtc gatatattcg agcaggcaca ccaggccgac cccgagtccg
     1081 atgctcgcga acagcccaaa gccgatcagc atcggccggt tcggcttcat cggcaccgtc
     1141 ggctcgacca cgctcgcggg gatccgtacg cagtcgctgt ggacgagttg ctgctggacg
     1201 attccaaact cgaggagcct ctgttccaac cccgaaatct gttttgcctt gtcctcgcgg
     1261 tccctcagta aggtaagcac gcgctggtgg tcgggcatca tctcttgcaa cttggccagc
     1321 agcttgtcgt gttgttcctt gtcggcgtcg agctcattgc ggtattgttc gatgaacatg
     1381 tcggtgtagt tggcggtctc tctgggcttg aaagcgctca gctctgcgat ctcgtcgagt
     1441 atggcttcga gcgacttggc ccactcttcc gcggcggggt cgtggttgaa attacgcatc
     1501 gttcgcttca tatggtcgag gccgcgctca tacttgcgct tgtctgccct caactgcgcg
     1561 agccgcgcct cgcggccgcc agcggccccg tcgagctcga acttgggaaa catctgcgaa
     1621 atcatcatct gctggtggag ttgattaatt tgcaactgct tttgcgacat catcgtgccc
     1681 aagttgatat attcgtcctc gagaatgttc ctgcctccag gccctacggt ccgggtcgtc
     1741 ttcatcgcct tgctgatctc ttcttcgagg gcggtcaggg cggttttcag gtttttgagg
     1801 ttttcctcgg cgtaaacctg ggtcgcgcgc agcttgtcgc cttgttccaa ctccgcctgc
     1861 ttcttgaatt cctccagcag gatttccagg agcctcttgg ttagagccgg gtcgcccccc
     1921 tcgagtgtca cgataaacga gttgccgttt ttctttactt ggaggacgga gagggacttg
     1981 aacagctcga gcgccgggtc ggcgaactgg gagaccgccg gcacgatgct atcgttactc
     2041 acgaccctct gggccagctc cttgctgcgc agccgcgctt cgcggttggg gatgtagctc
     2101 gcatgggctg cagggtcgct gcgaccaatc tcgtgcgtca ggagggccga aagcgttgga
     2161 tcgagctgcg gcggattgat ctcgatctcg gccttcacca cgtaaaccgg ggggagcctc
     2221 aagaccatga tcgacgacac gatggcaaac ggaacggcga ttgccagcac catccagatc
     2281 cgatggcgca cagccttgaa gtaatcggag gtggttttct gggcagcagg attgatgaat
     2341 tgcagcgacg cgcttgttgg cggccggcct gtactcggcg gaggagcatc tcctttgtag
     2401 ggttgaatcg cgtccataag cgcctctcta tctgatcgtt gaatcatccg cgggagaccg
     2461 aagctggtgt ggccagcgcg tcggcggccg ctcggttggg cgcctcgata tgggctttgg
     2521 ctgcccgctt gggcgtattc cggtcgacga cgcgggccaa gatcgtcccc aagaggccca
     2581 atgcggccag cgccgagccc tgtcctcgcg acgtaccgag ctcggggtcc ggcacggtgc
     2641 ccaaaaccgt tgtcttggtc atgctcgagg cggacgactc cagccgctcc agccggtcga
     2701 caagcgcctg gctcaagcgt tggaggtagc ggaagacgat gtcggcagcc agcactgaat
     2761 ccgacggca
//
LOCUS       DATAQL010000019         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1528762, whole genome shotgun sequence.
ACCESSION   DATAQL010000019 DATAQL010000000
VERSION     DATAQL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528762"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..353
                     /locus_tag="VHS97_00345"
                     /pseudo
     CDS             48..353
                     /locus_tag="VHS97_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            605..676
                     /locus_tag="VHS97_00350"
                     /pseudo
     CDS             605..676
                     /locus_tag="VHS97_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            806..1171
                     /locus_tag="VHS97_00355"
     CDS             806..1171
                     /locus_tag="VHS97_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446672.1"
                     /translation="MTLYDWVPTRARIHAIADEAGTRALCELFPGARLDVSVSYSSKD
                     RICQGRHKPLDAYQMIELSWGDGTNKSVRYCELLRTIMADQIAWIRNHSHARTITEDN
                     GRDSLAAACAADRLAQALV"
     gene            1196..>1983
                     /locus_tag="VHS97_00360"
     CDS             1196..>1983
                     /locus_tag="VHS97_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylase"
                     /protein_id="HEX3446673.1"
                     /translation="MLLKRHYERLLLSPSDLKPSRDDFEVVGVFNPGAIVAHGEVILL
                     VRVAERPRERRQGYMALPRWAPEDGLVIDWVADDVIQVLDPRVVKREADGLVRLTFIS
                     HIRVVRCGDGRSVKEVSDVRFSPATSLEEFGVEDPRITAIDGRYFFTYVAVSRHGAAT
                     ALASTADFRQFDRHGVIFCPENKDVVLFPERIGGQYVALHRPNAATPFCRPEMWIARS
                     PDLIHWGQHECLHGGGAEWETGRVGAGTPPVRVDAGWLEIYHGNR"
BASE COUNT          367 a          585 c          629 g          402 t
ORIGIN      
        1 acagcgatcc atgcacggac gaagtccgaa ctcgaaatgg agtaacgatg atttcaggag
       61 aaactcgact gggtgtaatt ggctgcgggg gtttcgggct ctacgccctt cagcagtttg
      121 cgcaggtacc tggcgtgaaa ctgacgggaa tggcgggtac ccaccgagat gccgcccgtg
      181 ccgccgcgcg gcggttcggc attcccgacg tccaggatgt cgaagcgctc ttggctcgcg
      241 acgacatcga catggtctac atcgcaacgc ctccattcct ccactttccg caagcgctcg
      301 ctgctcttga ggccggcaag cacgtcattt gcgaaaagcc tctggccatg acggtcgcgg
      361 aggccgatac cttgatcgcc atggcaagga agcgcgatcg ggtactcgtg gccaacttga
      421 tgcagcgata caaccccttg ttcgacgcca tcaagcacct gatccaagat caggtcctgg
      481 gcgagctgct tcacggatac tttgaaaact acgcgtcgga cgaaaatctg ccgccggagc
      541 actggttctg gaccgctcta aaagcggcgg aatcttcgtg gagcacggcg tccatttttt
      601 cgacatgttc gcgggctggt tgggacccgg tgtcgtcctg gccgcccagc gaagcgttcg
      661 tccgggctcg tcaccaccga tcgaggagca agtccaatgc accgttcgat acggtgcgac
      721 tgctctggtc aacttctttc atggatttga acaagccggc cgaatggacc ggcaggaatt
      781 gcggctcgtc ttcgagaggg gggacgtgac actttacgat tgggtgccta ccagggcccg
      841 catccacgcg atcgccgatg aggccggcac gcgcgctttg tgcgagctct tcccgggtgc
      901 gcggctcgat gtgtcggtga gctattcgag caaggacaga atctgccagg gtcggcacaa
      961 gccgctcgat gcctaccaga tgatcgagct ttcctgggga gatgggacca acaagtccgt
     1021 tcgctattgc gagctgctcc gaacgatcat ggcagaccag atcgcctgga tccgcaacca
     1081 ttcccacgcg cggacgatca ccgaggacaa tggccgcgat tcgctagctg ccgcctgcgc
     1141 cgccgaccgc ctcgcccagg ccttggtttg aaagtgaaca aggaaggacc cagccatgct
     1201 tttgaagcgg cattacgaac ggctgctttt gagtccgtct gatctgaaac cttcgcgcga
     1261 cgatttcgag gttgtcgggg tcttcaatcc tggggcaatc gtcgcgcacg gtgaggtgat
     1321 cctgctcgtc cgtgtggcgg agcgtccgcg cgagcgccgc cagggttata tggcactgcc
     1381 gcgatgggct cccgaagacg gtttggtgat cgactgggtt gccgacgacg tgatccaagt
     1441 gcttgatcca cgcgttgtca aacgcgaggc cgacggcctg gtacggctga cctttatctc
     1501 tcacatacga gttgtgaggt gcggtgatgg ccgctcggtg aaggaagtaa gcgacgtgcg
     1561 tttctcgcct gccacgagcc tggaagagtt cggagttgaa gatccgcgga tcacggcgat
     1621 cgatggccgg tattttttca cttacgtcgc ggtgtcacgt catggcgcgg cgaccgcgct
     1681 ggcgtcgacg gcggacttcc gccagttcga tcggcacggg gtgatttttt gtcccgagaa
     1741 caaggacgtt gtcttgttcc cggagcgaat cggcggccag tatgtcgcgc ttcatcgtcc
     1801 caacgccgcg acgccgtttt gccggcccga gatgtggatc gcacggtcgc cggacctgat
     1861 ccattggggc cagcatgagt gtctgcacgg cggcggtgcc gaatgggaga ccggccgcgt
     1921 cggcgcgggc acaccgcccg tgcgtgtcga cgccggatgg ctcgaaatct accacggcaa
     1981 ccg
//
LOCUS       DATAQL010000020         4000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3360714, whole genome shotgun sequence.
ACCESSION   DATAQL010000020 DATAQL010000000
VERSION     DATAQL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3360714"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..678)
                     /locus_tag="VHS97_00365"
     CDS             complement(415..678)
                     /locus_tag="VHS97_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEX3446674.1"
                     /translation="MDGPRAVGPSTKLFPIVEAAGSRWVSQRRGGGSFLSASDPRLHF
                     GLGPATRIDRLQVRWPSGQVDSFQNLDADKGYRIREGDPRPTE"
     gene            815..2737
                     /gene="ilvD"
                     /locus_tag="VHS97_00370"
     CDS             815..2737
                     /gene="ilvD"
                     /locus_tag="VHS97_00370"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3446675.1"
                     /translation="MRSDMIKRGDARAAHRSLLRATGVGEGDWDKPFIAICNSHVDII
                     PGHVHLQAVGNYVKECVRAAGGMPFLFNTIGVDDGIAMGHDGMKYSLPSRELIADCVE
                     TMIQAHCFDAMICIPNCDKIVPGMFMGAMRVNVPTIFVSGGPMEAGRTASGKTVDLID
                     AFVAGVQKQNGALSAEELDEIEKAACPTCGSCSGMFTANSMNCLAEAIGLALPGNGTI
                     LATSADRKSLYERASERIVAMAREFAKRGAGHGLLAREVASPAAFDNAMILDMAMGGS
                     TNTVLHILAIAHEAGVPFSLERIDELSKKTPNLCKVSPSSAYHIEDVARAGGIHTILG
                     EVARGCPGLLDLSCRTVTGKTLGENIDEFDIRRKTASSAAMLLTQVRAGGERTNKAWT
                     VPSVNEGRSQAAGLALLEAEGEVGDGLSDALGNGHGSGSNGHGDGDSFDPFDVIRTVE
                     HAYSATGGLTMLTGNLAPQGAVVKTAGVSARMLKHTGPAVIFDSEIDAYNGIVFDKVK
                     AGDVVVIRYEGPKGGPGMQEMLAPTTAIKGVGLGDSCALVTDGRFSGGTAGASIGHVS
                     PEAAVGGPIGLLKNGDVIEIDIPNGVLSVRLSEQELAKRRQDWTPPKPKVTSGYLARY
                     AKMATSADTGAILKWD"
     gene            complement(2875..>4000)
                     /locus_tag="VHS97_00375"
     CDS             complement(2875..>4000)
                     /locus_tag="VHS97_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446676.1"
                     /translation="LVARRTRVTPSCESLESRQLMDAAGVGSASALLEAQAAASTTQP
                     SHSFISLLPSSPTKSFSTIPANGDLNPYGVAKVPRGFLVTNFNNSQNLQGTGTTITKV
                     FLNGTTQTWFQAAQGFGLTDAIGVLSKGFVFVGSVPTTDGTSATVQQGSLLVLNRFGK
                     QIANLKNPNLLDGPWGMAVNDKGDTAQIFVSNVLNGTITRLNVTIKNGLPDITSAKQI
                     ARGYTIQSSASAVVLGPTGLAYNAQANVLYVASTADNAIYAIPNASSLNGPINKGIAV
                     VQNNTNLHGPIGLTFAPNGDLLVTNGDAVNPDPNNPSLLLEFRPRGAFVNSLSLDPAE
                     GAAFGLVVKKVGQSFWLGTVNDDTNTLDIRIASKSNLTSA"
BASE COUNT          788 a         1199 c         1264 g          749 t
ORIGIN      
        1 agcggttcgc cgggacccgg attcacgggg gcgatcggtg acgaatcccc gccgtcgggc
       61 ttgcggagga tgcgatggtt ggtcaccagc gcatggatga cgtcggtgcg atccgcccgc
      121 ggcatatggc agccaggtct cggcaatctt cccgtggcat tgagcacacg cggagtcccc
      181 cacatagcta accgccaagc gggtgttcac gtagggagag gcgatatcca ccatgtgcgg
      241 acggtttatc cccggagccc tccgccagga gacgaaggtc gtcgcggagc ccgccaccgc
      301 gaggttcgcg acgaccagca ggatcatgcg tctgtcgggg gtcatcggtt gctctgctgg
      361 tttatttgaa ggagtgaaaa ggcgtcagct actgagcatc tttttcactc tttttcactc
      421 cgtgggcctg gggtctccct ccctgatgcg atatccctta tctgcatcca ggttctggaa
      481 cgaatcgact tggcctgacg gccagcggac ttggagtcga tcgatccggg tcgcaggacc
      541 caggccgaaa tggagccggg ggtcgctcgc cgacagaaag ctgccgccgc cccgtcgttg
      601 cgacacccag cgcgaaccgg cggcctcgac gatcgggaaa agtttggtgc ttggcccaac
      661 ggcgcggggt ccatccaacc tcccggcgaa agcgatgcga gcttcgcgac ccgctcggca
      721 atcgtcttga gttcctcgaa tcgttatgat gaattctgtc gttcaaggcc tttcgcctcg
      781 caatgatgcc gcaaaccgct catggagccg cctaatgcgt tccgatatga tcaagcgtgg
      841 tgacgcccgc gctgcccacc gcagtctcct tcgagcaacc ggcgttggtg agggagattg
      901 ggacaagccc ttcatcgcga tctgcaacag ccatgtcgac atcattcccg gacacgtcca
      961 tctgcaggcg gtgggcaact acgtaaagga atgcgtacgg gcggccggtg gcatgccgtt
     1021 tttgttcaac acgatcggcg ttgacgacgg cattgccatg ggccacgacg gcatgaagta
     1081 ttcactcccc tcgcgcgagc tcatcgccga ctgcgtcgaa acgatgatcc aggcccactg
     1141 cttcgacgcg atgatctgca ttcccaactg cgacaagatc gtgccgggca tgttcatggg
     1201 agcgatgcgc gtcaacgtgc cgacgatatt cgtatccggg gggccgatgg aagccggtcg
     1261 gacagcctcg ggcaagaccg tcgatctgat cgacgcattt gtggcgggcg ttcaaaagca
     1321 gaacggcgcg ctttcggccg aggaactcga cgagatcgag aaggccgcct gtccgacctg
     1381 cggcagttgc tcgggcatgt tcacggccaa cagcatgaac tgcctggccg aggcgatcgg
     1441 cctggccctc cccggtaatg gcaccatttt ggcgacctcg gccgaccgca agtcgcttta
     1501 cgaacgggca tcggagcgca ttgtcgccat ggcacgtgaa ttcgccaaac gcggagccgg
     1561 ccacggactg ctggcccgcg aggtcgcctc accggcggca ttcgataacg ccatgatcct
     1621 cgacatggcg atggggggta gcaccaacac ggtcctgcac atcctggcga tcgcccacga
     1681 agccggtgtg ccgttttcgc tggagcgaat cgacgaattg agcaagaaga cccccaacct
     1741 ctgcaaggtg agcccgtcga gtgcttacca tatcgaagac gtcgcccgtg cggggggcat
     1801 tcacacgatc ctgggcgagg tcgcgcgggg atgtccaggc ttgctcgacc tttcatgccg
     1861 gacggtgacg ggcaagacgc tgggcgaaaa catcgacgag ttcgacatcc gccgcaagac
     1921 ggcgtcgtca gccgcgatgc tcctgaccca ggtgcgggcc ggcggcgagc gcaccaacaa
     1981 ggcctggact gtccccagcg ttaacgaagg tcgctcgcaa gccgctggac tcgccttgct
     2041 cgaagccgaa ggagaagtcg gagacggcct ttccgatgcg ctcggcaatg gccacggttc
     2101 cggatcaaac ggccacgggg acggcgacag cttcgatccg ttcgacgtga tccgcaccgt
     2161 cgagcacgcc tattcggcga ccggcggact gaccatgctc acgggaaatc tcgcgcccca
     2221 gggggccgtc gtcaagaccg ccggagtcag cgcccggatg ctcaaacaca ccggaccggc
     2281 ggtaatcttt gacagcgaga tcgacgccta taacggcatc gtatttgaca aggtcaaggc
     2341 gggggacgtg gttgtgatcc gctatgaggg gcccaagggg gggccgggca tgcaagagat
     2401 gctcgctccc acgacggcaa tcaaaggagt cgggctcggc gatagttgtg cgctcgtcac
     2461 cgacggccga ttctcgggtg ggacggccgg ggcctcgatc ggccacgtga gccccgaggc
     2521 cgccgtcggt ggcccgattg gtctcttgaa aaacggcgat gtgatcgaga tcgatattcc
     2581 aaacggagtc ctctccgtcc ggctttccga acaagagcta gctaagcgcc gccaagattg
     2641 gacgccgccc aagcccaagg tcacttcggg ctacctggcc cgctacgcca aaatggctac
     2701 cagcgccgac accggggcga tcctcaagtg ggattgagga tcgctcggca atcgaggaaa
     2761 tgccgaacga gcctcggccg cgtcgccacg taggtcaggc tttccggcct gacgtcagcc
     2821 tggaaatgct gacctacatg ggcggccccg cttcacaagc ggatgcgagt attctcacgc
     2881 actcgtcaaa ttgctcttgg atgcgatgcg gatgtcgagg gtattggtgt cgtcgttgac
     2941 ggttcccagc cagaagctct ggccaacctt cttgacgacc aacccgaaag ccgcaccttc
     3001 agccgggtcg agcgataatg aattgacgaa tgctcccctc ggccggaact cgagaagcag
     3061 gctcggatta ttcgggtcgg gattgacggc atcgccattg gtgacgagca agtcgccgtt
     3121 aggcgcgaag gtcagaccaa tcggaccatg caggttggtg ttattctgca cgacggcaat
     3181 ccccttgttg atcggcccgt tcagcgacga ggcgttgggg atggcgtaga tcgcgttgtc
     3241 ggccgtcgag gcgacgtaca gaacattagc ctgggcgtta taagccaaac ccgtgggtcc
     3301 aagcacaacc gccgaagcgc tcgactgaat cgtgtatccc ctcgcgattt gcttcgccga
     3361 tgtgatatcg ggcagaccat tcttgatggt cacgttcaaa cgcgtgatcg tgccgttcaa
     3421 gacgttggac acaaagatct gggccgtgtc ccccttgtcg ttgaccgcca tgccccaggg
     3481 cccatcgagt aaattggggt tcttgaggtt ggcgatctgt ttgccgaagc ggttgagcac
     3541 cagaagggaa ccctgctgga cggtcgccga cgtgccatcg gtcgtgggca cactaccgac
     3601 gaacacaaag cccttgctca agacgccaat ggcgtcggtg aggccgaagc cctgggcggc
     3661 ctgaaaccag gtttgggtgg taccattcaa gaaaaccttc gtgatcgtgg tcccggtgcc
     3721 ctgaaggttt tgggagttgt tgaagttggt tacaaggaac ccgcggggca ccttcgctac
     3781 gccgtaggga ttcaggtcgc cgtttgccgg tatcgtcgaa aaagatttgg tggggcttga
     3841 cggaagcaaa ctgataaatg aatgcgacgg ttgcgttgtc gaggcggccg cctgcgcctc
     3901 gagcagcgca gacgcggaac caactcctgc cgcatccatc agttgtcgcg attccagaga
     3961 ttcacacgat ggggttacgc gcgtgcgacg cgctaccagg
//
LOCUS       DATAQL010000021        11015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3208307, whole genome shotgun sequence.
ACCESSION   DATAQL010000021 DATAQL010000000
VERSION     DATAQL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11015
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3208307"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VHS97_00380"
     CDS             complement(<1..640)
                     /locus_tag="VHS97_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3446677.1"
                     /translation="MKSFLTACGLTNPLQLVVETQSSYEGELRLLNQPYAVIGRDPRA
                     DVVLDHAKVSRRHIYVQVVDGEAFWIDLESRTGTRGNGDSQKSGWLGGGGAVRAGPYV
                     IRRFTDESLADRQRVQSKLPEVAPLVAQAYGRAPLPEVTLEFLNGPAQSTIWPVRRVM
                     SLMGSASGCKFRLTDPSVSRFHGSMLRTAAGLWIVDLLGQGGITVNDRPVRFG"
     gene            967..1887
                     /gene="rfbA"
                     /locus_tag="VHS97_00385"
     CDS             967..1887
                     /gene="rfbA"
                     /locus_tag="VHS97_00385"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX3446678.1"
                     /translation="MNKVSMFRKGIILAGGSGTRLYPITRAVSKQLLPIYDKPMIYYP
                     LSSLMLAGIRQILLISTPEDIGSFERVLGDGNQFGVSITYAIQPKPGGLAQAFLIGRQ
                     FVGDDAVALILGDNIFYGQGFQAMLNRACAQTIGATVFAYPVKDPERYGVVEFDSQGK
                     VLSIEEKPTQPKSHYAVTGLYFYDNQVVEIATRLKPSERGELEITDVNRAYLNRGQLR
                     VEVFSRGFAWLDTGTTESLIQAANYVETIESRQGLKIACLEEIAYRMGYITAEQVEKL
                     ADKIPNDYGNYLREVIRELSTTTLAVDPHA"
     gene            complement(1902..6335)
                     /locus_tag="VHS97_00390"
     CDS             complement(1902..6335)
                     /locus_tag="VHS97_00390"
                     /inference="COORDINATES: protein motif:HMM:NF020855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3446679.1"
                     /translation="MRVKTIKRLAILIAVLGLVSGTAVWGWNWQITRMAHAKALEAES
                     AVKKGDFATAGRLYREHLALIPDDVEIQLAYADTLARGGSIAERAEALQVYGNVLRRS
                     RYIGRDDVRRKQMELKVKAGRLLDEGGAEFDLKMLLSNDANKNDGELWFWMGRCAEAG
                     KNDAAAATYYRSSIKADASPEIKIDAYWRLATVLRSRLDKPREADQAIEKMVESHPSN
                     YKVFLERGRYRRRFDLPDSAADFQKALQLSGGEPAVYVEMAKAAIAEGRQQNGAARQI
                     LEDGLKKWPSSAMLCRELAIRDLEIGRVDQAVETLERGLDTPAETRDTPERGLKISGE
                     VVDKADLLWLLARVLARSGDTAKLRLRIEQLKGVGVQTTLVDFLTAHYYVNTKDFRKA
                     RQILVPIDSKTGLPADIRAMVKDLLAICHSQLGEPGKQQEFYRQALAANPQDSRAKQG
                     LIDSMVKQGDIEGAIKEYNKNRTTLTPGERLHLAQLLIVRNRQRSKPQRDWSEAAALI
                     DDASSSSPELTEPAMRLRAELCIAQEKPAEAKDILEKARSQFPKSVALWVAAADLMAT
                     QKQFDAASALLAQAKDQLGSDRVELQLERAKLAVAEGGPQVAAKLNALGQDIDAFPKE
                     DRARILNALGLDLYQLQDFPAAGRFWSRMAEQEPNNLDLRLRLLDVACQTANIAEIDD
                     NIKKIKEIEGNDGSIGRYCDINYLIWQAQRAMDKDPREAQRLRTKARSDLNDLDARRP
                     DWSHVPLTMAKIEQQELLQPGLRDDEIRANEENIIRSYLKAIDLGEHSSAVLRSTVQL
                     LFKNKRGIEAIDLINRIPLESQLSADLTRLANQFALANRDFKGAEDIARKAVAANPDD
                     FMNQFWLVRVLLQSGKQEDALTVLRNAVDRAPKEPDRWLTLVQFLLMTRQPDQAAKAI
                     NTAEANLPPDRAPLALAQYCELMVRFYGAANEQATTKWLANARSWYQKAQAAHPDDIS
                     IARRITEFYLGAKLVAEAESELDAILRRDAGASSDQIRAWARRTKALILASSTDPRRV
                     RSALAVLEPPGQAALSGDGQKALDDPDDLRVLAQVLSYQRTEQDRQRAIDILNSLVTK
                     NTAKPDDRFLLAQLYEASGIWDKAREEYLALNAKTRSARDLETLNRRPQYLLHFAAKS
                     LLRNRTARNEQALSDVQDVIDDLKQIQPDAIATLALQVDLYRARNQLDAAADLIRTTA
                     DRPDLAPSMLKQLAELAERIDKLDLAEQVHKKYADSSNSPLQKYPLVIFLAKNDRLEK
                     ALDICEPLWANPRDMESVAQICIDAISYSSKYDRAQFERAAAWLEKAREAAETQNWNS
                     TSTVLSALASIRERQERFQDAEALYKHAIDKGHNPIAYNNLAWLMVLRNGGAAKEALA
                     YVERAIALGGPLRSYLDTRAVIYMALGQPQKAISDLEKVVETDPSPAELFHLAQAYLQ
                     ANKQQKAKEDLRAAKDKGLPKGLHSLEMSAYKKLLEDLGPL"
     gene            7322..>11015
                     /locus_tag="VHS97_00395"
     CDS             7322..>11015
                     /locus_tag="VHS97_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446680.1"
                     /translation="MRRGSSEILSRGHRGRSRLDARGKTTRHLRQCQIDLEGLESRTL
                     LATIPAATATGAPIDLSGLSTVTNNGNAHSPTVVVDPYDSQKLFAVWGVDLSQVVPAP
                     TPTTAIVEGAFSNNGGATWNNLGEQVASRQLDVATINATPPTNYTQITEPSVAFDGNG
                     DVYVLSLQTSGANDGLLYLTQFNFSGATPVLTSAYNVYQWVSGSDAATDAVLSVDTAN
                     PGAPGAPDPHANNVYIAWASIDAIPASTLPTVFNPNRAELVVGTPNSIGGNQVRLAFS
                     AVTTVNAGGNFGGAPNGGNDDAHPQLVINQTNGGKVTVLWDDFGSGSKASPAFDNLLS
                     NLVQPGDTYGFAGATGPFLNGIPSPPAAAPTPVPTFFTDSVTVPNQAAIDNLSVTIDL
                     VDQQTVQNLNLTLIAPDGVHSIVLVQNQNNAAGTANTGQGLPSGNAIGVFGFATGATG
                     TPGRNVGTIFDDNATRNIFDPTTTGTNGNNATNYIGTFRPEGGSLQSFLSSLGGAING
                     TWTLQITNFASSAVTGFLNPGQLQHFSLQFSTGMSRSSPSVVATTLVTGALGNSFANK
                     PPSSPSVGVGPGPVVAIDNTLGSASPDQGRIYAAYVIYLRDSDPNAHLNPTTNTDIAL
                     SYSDNGGQTWISDGIVNDDAATLDGYSGSSIGSASAYTSGRTQFQPEIAVDQATGTLV
                     VSWRDARNDAANARVATYITTSIDGGNSFSAQTYANPSQTAIDAITGQTDILGPLGDN
                     QSSGNGGQADPTFGYGDQMGLAVSNGHVFPVWAGNFNAAYLVNTSTVTAYPLNIWYRP
                     MVIAAGPRIVTSSMGPIPYAEAAAGNHLVSISVTFDRPVDPNTVSVGDVKVFYHDTSN
                     TSSSIALPVASFLPTAGGTSSTTQFTIVFNAAAAGPIYTGTYSYLIAPDNGSGLIIST
                     PVESYKGTVLGTYDRMDQNADGTSDQNAVTTAFTGLTPGDVYAAPAPQPLTPIQFLGA
                     ASILTPPFNQNTLPLIVPGPQALSTSVPGGNSAAGNLITDGTTSTLSVTFDRPMETSS
                     FTPSQVLQIMGPAGAISGPQFFPSTSSTGQIIPAATSAGAGVLNSTLTIPSFGGTFKA
                     AHIIVDLNAAFSPDSQLTGVLIAPNGTTVTLFSGVGATGANFINTIFDDSAASSITTG
                     TAPFTGTFQPTGKLSTLNGLTVDMQNSALQWVPGVWTLQLSNASTGATGMLANWSLNI
                     TPVITVTPVSPVSGAATTFTIGFPLQQ"
BASE COUNT         2240 a         3391 c         3023 g         2361 t
ORIGIN      
        1 ggccgaagcg cactggccta tcgttgaccg tgatgccccc ctgccccagc aggtctacaa
       61 tccacaagcc agccgcggtg cgcagcattg aaccatggaa tcgagaaaca ctgggatcag
      121 tgagtctgaa cttacaacca ctggcggatc ccatcaacga catcacgcgg cgaacgggcc
      181 aaatagtcga ttgtgccgga ccgttaagaa actcgagcgt gacctccggt aatggtgcgc
      241 ggccgtaggc ctgtgcaacc agaggagcaa cttctggcag cttgctttgt actcgctggc
      301 ggtcagccaa gctctcgtcg gtaaaacgtc ggattacata aggaccggcg cgaacggccc
      361 ctcccccccc cagccagccc gacttttgcg agtctccatt accgcgcgtc cccgtccggc
      421 tctccagatc aatccagaac gcctcaccgt cgaccacttg aacataaatg tgccgccggc
      481 tcactttggc gtgatcgaga accacgtccg cccgcggatc acggccgata accgcgtacg
      541 gctgattcaa cagccgcaat tcgccctcgt aagagctctg agtctcaaca acgagctgga
      601 ggggattcgt aagtccacag gcggtcaaga aagatttcat cactctgggt gatctaaccg
      661 tgaactcggg cagcagccac ggttggtcac catcactcct tcccgaatcg agaactacat
      721 ggcgcaccaa ccgctcgtgg cggctcgccc agagagtgga gacgaatcgg gcgtccggcg
      781 atcaaaccca tgcatcgatt gcgggcttga cccattatca gaaacgatcg tgccgttgtc
      841 cacgagagaa ttatggagta aaacgacaac caaatcgcag gcgcgaattg tccggccgtg
      901 ctgaacgcct cgcagtcgtg gattcgactt gagctgatac taactccagc gggaaaggcg
      961 actttaatga ataaggtgag catgtttcgc aaaggcatca ttctggccgg cggttctgga
     1021 acacggctgt atccgatcac gcgtgcagtc agcaaacaat tgttgccgat ctacgacaaa
     1081 ccgatgatct attatccgct atcgagcttg atgctggctg gaattcgcca aattttgctg
     1141 atctcgacac cagaggacat cggcagtttc gaacgcgtgc taggcgatgg caaccagttc
     1201 ggggtttcga tcacgtatgc gatccagccc aaaccgggtg gcctggccca ggcttttctg
     1261 atcgggcgtc aattcgtggg cgacgatgcc gtcgcgctga ttctcggcga taacattttc
     1321 tacggccagg gatttcaggc catgctcaat cgtgcatgcg ctcaaactat cggagcgacg
     1381 gtcttcgcgt atcccgtcaa ggatccagag cgctacggcg tggtcgaatt cgattctcaa
     1441 ggcaaggtcc tatccatcga agagaaaccc acgcaaccca agtcgcacta tgccgtcacc
     1501 ggactttatt tttacgacaa ccaggtcgtc gagattgcca cgcggttgaa accttctgag
     1561 cgaggggaac tggaaatcac cgacgtcaat cgcgcgtact taaatcgtgg tcaattgcgc
     1621 gtcgaggtat tcagtcgcgg cttcgcctgg cttgacacag gcacgacaga gtctctcatc
     1681 caggctgcca attatgtcga aaccattgaa tcacgccaag gattgaagat cgcgtgcttg
     1741 gaggagatcg cctaccgcat ggggtatatc acggccgagc aagtagagaa gctggccgac
     1801 aaaattccca acgactatgg aaactacctt cgagaagtca tccgggaact gtcgacgacg
     1861 acgctcgcgg tggatccaca tgcctgattc tggtgcactc gtcatagcgg tcccaggtct
     1921 tcaagtaact ttttataggc cgacatttcc agcgaatgta atccttttgg cagccccttg
     1981 tccttggccg cccgcagatc ctctttcgcc ttttgctgtt tgttagcttg aagataggcc
     2041 tgcgccaggt gaaacaactc agctggtgaa ggatcagtct cgaccacttt ttcaaggtcg
     2101 ctgatggctt tttggggttg acccaaggcc atgtagatga cggcccgggt gtcgagataa
     2161 cttcgcaacg gaccgcccag cgcgatggcg cgttcgacgt acgcaagagc ttcttttgcc
     2221 gccccgccat tgcgaaggac catcagccac gcgaggttat tgtacgcgat cgggttgtgt
     2281 cccttgtcga ttgcgtgctt gtacaacgct tccgcatcct ggaagcgttc ctgccgctcg
     2341 cgaatgctgg ccagagcaga cagcactgtt gaagtcgaat tccaattctg agtctccgcg
     2401 gcttcccggg ccttctcgag ccaggcggcg gcgcgctcga actgcgcccg gtcgtatttg
     2461 gaagagtacg agatggcgtc gatgcaaatc tgggcgaccg actccatgtc acgtggattc
     2521 gcccaaagtg gttcgcagat atcgagggct ttctcgagtc ggtcgttttt ggcgagaaaa
     2581 ataaccaagg ggtacttttg cagcggcgag ttcgacgagt ccgcgtactt tttatgaact
     2641 tgctcggcca ggtcaagttt atcgattctt tcggccaatt cagccagctg tttcagcatc
     2701 gacggggcta gatctggccg atcggcagtg gtccgaatca gatccgcggc cgcatcaagt
     2761 tggttgcgag cgcggtagag atccacctga agggcgagag ttgcgatcgc gtcgggctga
     2821 atctgtttga gatcgtcgat gacatcctgc acatcagaaa gcgcttgttc gttgcgggca
     2881 gtgcgatttc gtagaaggct tttcgccgcg aaatggagca ggtattgggg cctgcggttt
     2941 aaggtctcca ggtcgcgtgc gctcctggtt ttcgcattga gcgccaggta ttcttcgcgt
     3001 gctttgtccc aaatgccgct ggcctcgtag agttgcgcca gcaggaatcg gtcatcgggt
     3061 ttggcggtat tcttggtgac gagcgaattc aagatatcga tcgcccgctg ccgatcttgc
     3121 tcagtccgtt ggtagcttag cacctgagcc aaaacccgca ggtcgtctgg atcgtcgaga
     3181 gctttctggc cgtcgccgga aagggcggcc tgaccgggcg gctcgagcac tgccaaggca
     3241 ctccggaccc gccgcgggtc ggtcgacgac gcaagaatca aggctttggt gcgccgagcc
     3301 cacgccctta tctggtcgct gctggcgccg gcgtcccgcc tcaagatcgc gtccaattct
     3361 gactcggcct ccgcgaccag cttagccccc agataaaact ctgtaatacg gcgggcgatc
     3421 gagatgtcgt cgggatgagc ggcttgcgcc ttttggtacc agctcctggc attggcgagc
     3481 cacttcgtcg ttgcctgttc attggccgct ccgtagaacc gtaccatcaa ttcacagtat
     3541 tgcgccaagg ccaacggcgc tcgatccgga ggcagattcg cctcggcagt attgatagct
     3601 ttcgctgcct gatccggctg cctggtcatc aacaggaact ggaccagagt gagccatcgg
     3661 tcaggttctt taggagcgcg gtcaaccgcg tttcggagca ccgtgagagc gtcctcctgc
     3721 ttcccactct ggagcaaaac ccggactagc cagaattggt tcatgaaatc gtccggattg
     3781 gcggcgaccg ccttgcgcgc gatatcctcg gcgcccttga agtcgcgatt cgcgagtgcg
     3841 aattgattcg ccaaccgcgt caggtcggca ctgagctggg actcgagggg aatcctattg
     3901 ataaggtcga tcgcctcaat gccccgcttg tttttgaaca agagctgaac ggtgctgcgg
     3961 agtactgccg agctgtgctc gcccagatcg atcgccttca agtacgagcg aatgatgttc
     4021 tcttcgttcg cacggatttc gtcgtccctc aggcctggtt ggagcagctc ttgctgctca
     4081 attttagcca tcgtcaaggg aacatgagac cagtccggac ggcgtgcatc cagatcatta
     4141 aggtcgcttc gcgccttcgt tcgtagtcgc tgagcttcgc gcggatcctt atccatggct
     4201 cgctgcgcct gccagatcag ataattgata tcgcaataac gaccgatcga cccgtcattc
     4261 ccttcgatct ccttgatttt cttgatgttg tcatcgatct ctgcgatgtt ggcggtctga
     4321 cacgccacgt cgagtagacg aagccgtaaa tcgagattgt taggctcttg ttcggccatg
     4381 cgtgaccaga atcggcccgc cgctggaaaa tcttgcagtt gatagaggtc aaggcctaat
     4441 gcgttcagta ttcgggcgcg atcttccttg ggaaacgcgt caatgtcctg tcccaacgcg
     4501 ttcaatttcg cggcgacctg cggccctccc tcggcgaccg cgagcttcgc gcgttccagc
     4561 tgaagttcga cccgatcgct gccgagttgg tctttagctt gagcgagtaa agcggaagct
     4621 gcgtcaaact gcttttgagt cgccatcagg tcggcggcag cgacccaaag cgccacactc
     4681 ttgggaaatt gcgatcgagc tttttcgaga atgtctttcg cttccgcggg cttttcttgt
     4741 gcgatgcaca gctccgcgcg caggcgcatc gccggttcgg tcaattcggg tgagctactt
     4801 gacgcatcgt cgatcagggc ggcagcctca ctccagtcgc gctgcggctt tgatcgctgg
     4861 cggttgcgta caatcaacag ttgtgccaga tgcaaccttt ccccgggcgt cagcgtggtc
     4921 cggttcttat tgtattcctt gatcgcccct tcgatgtccc cctgtttgac catggagtcg
     4981 atcaacccct gcttcgctct cgaatcttgg gggttggccg ctagcgcctg acgatagaat
     5041 tcttgctgct tgccgggttc gcccaactgg ctgtggcaaa tcgctaataa atccttgacc
     5101 attgcccgga tatcggctgg tagaccggtt tttgagtcga tcgggacgag gatttggcgc
     5161 gccttgcgaa aatctttcgt atttacataa tagtgcgcgg tcagaaaatc cactaacgtg
     5221 gtctggacgc cgacgccctt gagttgctcg atccgcagtc gtagtttggc ggtgtctccg
     5281 ctacgggcta aaactctcgc caagagccag agaagatcgg ctttatcgac gacctcacct
     5341 gaaatcttga ggccgcgctc gggcgtgtcg cgcgtctcgg caggcgtatc caggccgcgc
     5401 tccagggttt cgaccgcctg atcaacgcgg ccgatttcga gatcgcgtat ggccaattcc
     5461 cggcaaagca ttgcggagga cggccatttt ttcaggccgt cctcgaggat ttgtcgtgca
     5521 gcgccgttct gctggcgacc ttcggcgatc gcagccttgg ccatctccac gtacaccgca
     5581 ggctcgccgc ccgacaattg cagtgctttt tgaaagtcgg ccgcgctgtc cggcagatcg
     5641 aaccggcggc ggtaacggcc gcgctcgagg aagaccttgt agttacttgg atgggactcg
     5701 accatttttt cgatcgcttg gtccgcctct cggggcttat ccagccggct gcggagcacg
     5761 gtcgcgagcc gccagtacgc gtcgatctta atctctggtg aggcgtcagc cttgatcgaa
     5821 ctccggtagt acgtcgctgc ggccgcgtcg tttttccctg cctcagcaca acggcccatc
     5881 caaaaccaga gctccccatc gttcttgttc gcgtcgttgc tcaaaagcat cttcaagtcg
     5941 aattcggcgc caccctcgtc gagcagacgc ccagccttca ccttcaactc catctgtttg
     6001 cggcgcacgt cgtcgcgccc gatataccgc gaccgcctga gaacattgcc gtaaacttgc
     6061 agcgcctcgg cacgctcggc gatcgatccg cctcttgcca gtgtgtcggc atacgcgagt
     6121 tgtatctcga catcatcggg tataagcgcc aggtgctctc gataaagccg tcctgcggtg
     6181 gcaaagtcac ccttcttgac ggcgctctcg gcctccagag ccttggcgtg ggccatcctt
     6241 gtgatttgcc aattccaacc ccatacggcc gtaccactga cgagtcccag aaccgcaatt
     6301 aagatcgcca ggcgcttgat agtttttact cgcataacaa tttcgtcctc aacgttgccg
     6361 gttgccgaga gattgctccg gacgcgatcc aggctgtttc gtttataaag agcttacggt
     6421 aattcccacc aaactaatca aacgccatca cacctgacca tccattctag gtcgagccag
     6481 ctcgagtcta ggtcgaacga gctcgacgct gttggttttt ggcaacgcca actacgtcgg
     6541 agttagccag ctatggccac tcaatgttgg catcagggga agactagcga ccatccggtc
     6601 atattaattc cgtccctcat ggcttcttga tcgctgttga ccgtggtacc atggtgcatg
     6661 agccaccgat tgtgccatgg gagcgatgcg tcaatgcttc tgagctgccc ggttcacatg
     6721 gagcgacggc tggcttggtc gtgcaataag agcagaaacc agacggatga cggtggatga
     6781 tcaaacgtat ctaccgcaac ttcggtttct gccgacgact gaggacaaag atcccccgca
     6841 gtcaaaggac ttactcatct tagatgacgg gttgacctgt atgcgtgacg accgctgatc
     6901 ctgcctaatt gcgagcttac tttcttcagc ggattgcggc gtgatgccct acctcaacct
     6961 aattgcgcta gtgcccgcaa ttttcctggg aaaaatccaa gaaaattttc gacaccctat
     7021 tgacacgcta acacccaagc gtgaatgcta gcaacacagt atgcaggcag tcatgcgacc
     7081 ggccaaggct tgaaagctca acgagtgagc ggatcaggtt gtgacaccag gatatcgagc
     7141 gagccgaaaa ccgcgcgttg cagacgaaga aatttgacac cgacccgtta agcctgcatg
     7201 ctttctcgat tccttgagct ttatctcggc tttgcgatct acgcagatta cgcaatcgga
     7261 atcaccgcca caaatatcgc tctccgaaga gcgatcaaca gagagactgg gggaccaggt
     7321 tatgcgtagg ggatcgtcgg aaatcctttc tcgggggcac cgcggtcgga gtcgcctgga
     7381 tgccaggggc aagacaactc gccatttacg gcaatgccag atcgacctgg agggacttga
     7441 gtcgcgcacg ctgctggcca cgataccggc ggccactgcg acaggtgcgc cgatcgatct
     7501 ttccggtctg tcgactgtga cgaacaatgg caacgcccac agtccgacgg tggtggttga
     7561 cccttacgat tcccagaagc tcttcgcggt atggggcgtc gatctgtcgc aagtggtccc
     7621 tgctcctacg ccgacgacgg cgatcgtcga gggggctttt tccaataacg gtggcgcgac
     7681 ctggaacaat ctcggcgaac aagtcgcttc ccggcagctc gacgtggcca cgatcaacgc
     7741 cacccccccg actaactata cccagataac cgaacccagt gtcgcctttg acggcaatgg
     7801 tgatgtttac gtactctccc ttcaaacgag cggtgccaac gacggcttac tctacctgac
     7861 ccagttcaac ttctccggcg ctacgccggt actcacgtcg gcctacaatg tataccaatg
     7921 ggtcagcggc tccgacgcag cgacggacgc agtcttgtcc gtcgacacgg cgaatcccgg
     7981 cgcgcccggc gcccccgacc ctcatgcgaa caatgtctac atcgcctggg caagcatcga
     8041 tgccattccc gcctctactc tcccgaccgt cttcaatccg aacagggccg agctggtcgt
     8101 cggaacgccc aattccattg gagggaacca ggtacgatta gcgttcagtg ccgtgacgac
     8161 cgtcaatgcg ggtggcaact tcggcggcgc ccccaacggc gggaacgacg atgcgcaccc
     8221 gcaactcgtg atcaaccaga cgaatggcgg caaggtcact gtactctggg atgacttcgg
     8281 ctcaggttcc aaggcctcgc cggcgttcga taatctcttg tcaaacctgg tccagcccgg
     8341 cgatacgtac ggattcgccg gcgccaccgg gccgttcctt aatggcatcc ccagcccccc
     8401 ggcagcagca cccacaccgg taccgacctt tttcaccgat tcagtcaccg tccccaacca
     8461 ggccgcaatc gacaacctct ccgtcacgat tgacctggtc gatcagcaga ctgtccagaa
     8521 tctgaatctc acgttgattg ctcccgatgg ggttcactcg atcgtcctgg tccagaacca
     8581 gaacaatgcc gccggcacag ccaacacagg tcagggactt cctagcggca atgccattgg
     8641 agtgtttggt ttcgctacgg gtgccaccgg tactcccggg cgcaacgtcg ggacgatctt
     8701 cgacgacaac gccacccgga acatcttcga cccgacgacg acagggacga acggcaacaa
     8761 cgcaacgaac tatattggca ccttccggcc tgaaggaggt agcctgcaga gtttcctttc
     8821 gtctttaggg ggcgccatca atggcacctg gacactgcag atcacaaatt tcgcgtcctc
     8881 ggccgtcacc ggtttcctca acccgggtca gcttcaacat tttagcctcc agttcagtac
     8941 ggggatgagc cgcagttcgc caagcgtggt cgccacgacc ctcgtcacgg gtgcactcgg
     9001 caatagcttt gcaaacaagc cgccgtcctc gcccagcgtg ggagttggcc caggcccggt
     9061 ggtcgccatc gacaacactc tgggctccgc cagccccgat caagggcgga tctacgctgc
     9121 ctatgtcatc tacttgaggg actccgatcc aaatgcgcac ctcaatccga ccaccaatac
     9181 cgatatcgcc ctgagctact ccgacaacgg cggccaaacg tggatctccg acggcattgt
     9241 taatgacgac gccgcgacct tggacggcta ctcggggtcg agcatcggct ccgcttccgc
     9301 ctacacgagt ggccggacgc agtttcagcc cgagattgcg gtcgaccagg cgacaggcac
     9361 tctcgtcgtc tcctggcgcg acgctcgcaa cgatgcggct aatgcccggg tggccaccta
     9421 catcaccacc agcatcgacg gcggcaattc ttttagcgcc cagacctatg ccaacccgtc
     9481 ccagaccgcg atcgacgcca tcacgggtca gacagatatc ctgggcccct tgggcgataa
     9541 tcagtcgagc ggaaatggtg gccaggccga tcctacattc ggttacgggg accagatggg
     9601 gctagccgtc tccaatggtc atgtcttccc ggtctgggcg ggcaacttca acgcggccta
     9661 cctcgtcaac acatcgacag tcacggcgta tccgctcaac atctggtacc ggccaatggt
     9721 catcgcggcc gggccgcgga tcgtcacaag ctcgatggga ccgatcccgt acgctgaggc
     9781 ggcggcgggc aatcatctgg tcagcatctc cgtaaccttc gaccgtccag tcgatcccaa
     9841 cacggtctcg gtaggtgacg ttaaggtctt ctaccacgac acgagcaata ccagtagctc
     9901 gatcgcgctg ccagtggcaa gtttcctccc gaccgcgggc gggaccagct ccacgacgca
     9961 gttcacaatc gtctttaacg ctgcggccgc cgggccaatc tatacaggca cctacagcta
    10021 tttgatcgct ccggacaacg gttcaggcct tattatcagc accccggtcg agagctataa
    10081 gggcaccgtc cttggcacgt acgatcggat ggatcagaat gccgacggaa catccgacca
    10141 gaacgcggtg acaacagcgt tcaccggcct gacgccaggc gacgtctacg cggccccggc
    10201 gccccaacct ctgaccccga ttcaattctt aggcgccgcc agcatcctca ctccaccgtt
    10261 caatcaaaac accctgccgc tgatcgtgcc cgggcctcaa gctctcagca cctccgtgcc
    10321 gggcggtaac agcgccgccg gtaacctgat caccgatgga acgacgagca cactcagcgt
    10381 gacgttcgac cggcccatgg agacaagctc cttcacgccg agccaggtgc tgcagatcat
    10441 gggaccagct ggcgcgatct ccggtccgca gttcttcccg tcgaccagta gcactggcca
    10501 gataatcccg gccgccacct cggccggggc gggcgtgctc aactcgacgt tgaccattcc
    10561 gagcttcggt ggcacgttca aggctgctca tatcatcgtc gacctcaacg ccgcgttctc
    10621 gccggattca caactcaccg gagtcctgat cgcgccaaac gggaccacgg tcaccttgtt
    10681 ctcgggagtg ggcgccaccg gcgcgaactt catcaacacc atctttgacg actcggccgc
    10741 gagttcgatc accacgggca cagccccatt taccgggact tttcagccta cgggcaagct
    10801 ctcgacactg aacggcctta cggtcgatat gcaaaactcc gcccttcagt gggtccctgg
    10861 tgtctggacg ctccagctct ccaacgcctc gaccggcgcc accggcatgc tcgccaactg
    10921 gtcgctcaat atcacgccgg tgatcactgt cacgccggtc agccccgtga gcggggccgc
    10981 gaccacgttc acgatcggct tcccgctcca gcagc
//
LOCUS       DATAQL010000022         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1986919, whole genome shotgun sequence.
ACCESSION   DATAQL010000022 DATAQL010000000
VERSION     DATAQL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986919"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1050)
                     /locus_tag="VHS97_00400"
     CDS             complement(10..1050)
                     /locus_tag="VHS97_00400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX3446681.1"
                     /translation="MRIGLGINYAGGFKEVAAEVADLERAGLDIVFVPEAYSFDAVSA
                     LGYLAACTERVQLASGILQLYTRTPTLTAMTAAGLDYVSDGRFTLGLGASGPQVIEGF
                     HGVPYDAPIARTREVIDICRQVWRREYLQHKGKHYTIPLPADQGTGLGKALKLINEPV
                     RERIPILVAALGPKNVELVAEVAEGWQPIFYLPEKAPEVWGQALATGRAKRDPALGEL
                     DVYAGPVLAIGENVEPLREFVKPHLALYIGGMGAKGKNFYHALATKYGYGPQADRIQE
                     LYLSGDKDGAAKVVPDELVRDVNLIGTREFVKERLAAFREAGVTTLNVAPIAATPAER
                     IKLIETLRDLVD"
     gene            complement(1063..1485)
                     /locus_tag="VHS97_00405"
     CDS             complement(1063..1485)
                     /locus_tag="VHS97_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3446682.1"
                     /translation="MHPFRKAVEERDEAAIQALLADDVVFTSPVAFKPYVGKPITAAL
                     LRGVLRIFEDFRYVREIADANGRDHALVFEARISGADGKKVNGCDFLHFNDDGLIDDF
                     MVMVRPLSGATALSEAMGAQFDRIQQEALELANQFTSA"
BASE COUNT          289 a          542 c          524 g          331 t
ORIGIN      
        1 gcaacggcgt tagtcaacca ggtcgcgaag cgtctcgatc agcttgatcc gctcggccgg
       61 cgtagccgcg atgggtgcca cgttcagggt cgtcactccc gcctcgcgga acgccgccaa
      121 acgttccttg acgaactcgc gggtaccgat caggttgacg tcccgcacga gttcgtcggg
      181 cacgaccttg gccgcgccgt ccttgtcgcc ggacaggtag agctcttgga tccggtcggc
      241 ttgagggccg tagccgtact tcgtggccag ggcgtggtag aagttcttgc ccttggcgcc
      301 cattccgccg atgtataggg ccaggtgcgg tttgacgaat tcccgtagtg gctcgacgtt
      361 ttcaccgatg gccagtaccg gtccggcata cacgtcaagc tcacccaagg cggggtcccg
      421 cttggcccgg ccggtcgcca gcgcctgtcc ccacacctcc ggcgcctttt cgggcagata
      481 gaagatcggc tgccagccct cggcgacctc ggccaccagc tcgacgtttt ttggtcccag
      541 cgcggccacc aagatcggaa tacgttcccg gacaggctca ttgatgagtt tcagcgcctt
      601 accgagtccg gtgccctgat cggcgggcaa cggtatcgtg tagtgcttgc ccttgtgttg
      661 caggtactca cgacgccaca cctggcggca aatgtcgatg acttcgcggg tgcgggcaat
      721 cggggcgtcg tagggcacgc cgtggaagcc ctcgatgacc tgtgggccag aggcgccgag
      781 gccgagcgtg aaccggccat cggagacgta atcgaggccg gccgcggtca tcgcggtcag
      841 cgtgggcgtt cgggtgtaca gctgcaagat gcccgaggcc aactgaaccc gttctgtgca
      901 ggcggccagg taccccagcg cgctcaccgc gtcgaacgaa tatgcctcgg ggacaaagac
      961 aatgtccaac ccggcgcgtt cgaggtcggc cacttcggcg gccacctcct tgaacccgcc
     1021 cgcgtagttg atccccaatc cgatccgcat cttttcctac ccctacgcgc tggtgaactg
     1081 attggccagt tcgagagcct cttgctgaat ccgatcgaac tgggcgccca tcgcctcgga
     1141 caatgcagtc gcgcccgaca gcggccgcac catcaccatg aagtcgtcga tcagaccatc
     1201 gtcgttgaag tgcaggaagt cgcacccgtt gaccttcttg ccgtccgcgc cggaaatcct
     1261 ggcctcgaag acgagcgcgt ggtcgcggcc gttggcgtcg gcaatctccc tgacgtagcg
     1321 gaaatcctcg aagatgcgca acacaccgcg caggagcgcc gcggtgatcg gcttgccgac
     1381 atacggcttg aatgccactg ggctggtgaa caccacgtcg tcggccagca gcgcctggat
     1441 ggccgcctcg tcgcgctcct cgactgcttt gcggaaggga tgcatcagac acctcgcata
     1501 gtcgacttgt tgagtaggtg gggttgacgc tagagcctgt tcggccgcat gtcgatatgc
     1561 gattagtcac tttgttgatt attcacatcg ttgtatgatc ggccgatgtc gctccgtgac
     1621 gcggtgttgg ccaccctcct ggacggcgag ttctccggat acgacctggc caagagattc
     1681 gacgcc
//
LOCUS       DATAQL010000023         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5193183, whole genome shotgun sequence.
ACCESSION   DATAQL010000023 DATAQL010000000
VERSION     DATAQL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5193183"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(563..1663)
                     /locus_tag="VHS97_00410"
     CDS             complement(563..1663)
                     /locus_tag="VHS97_00410"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX3446683.1"
                     /translation="MKPLQVADYKPVWIICLVASAIGAGLAAKSASASDDADLLKDAR
                     GLFQPLPKDMATKEFPVTPERVLLGRMLFFDPRISADGTGSCMRCHQPALYGADGLPK
                     SVGVHDKPLPRNAPTVFNAGLYTSEHWDGRFANVEAQAKSALLGPGFGNPDFPTAMAR
                     VKAIPGYGAMFQQAFPGEADPVSEDNWGKAIGAYERTLVSPSRFDDYLAGKSDALSAA
                     ERKGLRTFIDTGCVECHKGSGLGGLGFRKFGVVSDYWPLTGSQDIDKGRFVLTNEPAD
                     LYKFKVAGLRNVAMTPPYFHDGAVDTLPKAVHIMAKIQLDADLSDQEVNEIVTFLGSL
                     TGTLPEGFERAPVLPAGGFRPPTSASPGPQTR"
     gene            complement(1853..>2179)
                     /locus_tag="VHS97_00415"
     CDS             complement(1853..>2179)
                     /locus_tag="VHS97_00415"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEX3446684.1"
                     /translation="RPLGEVVKGQSVWTHAEVCGTLVGVRCPAWVEGLNVPGYHWHFL
                     SDDRKVGGHVLDCRVDEGRVHFDVCVNWLIKLDGSAELNAVNLGEDLSRDLRRVESSR
                     GENNSQ"
BASE COUNT          418 a          673 c          651 g          437 t
ORIGIN      
        1 cggcaccgcc ctgagtcggt agagttgccg tgccaaccct cgctctacaa ctgtccattc
       61 gaacagatat cgaggtgttc acagctcttg gagcttctcg cagcggctgg gaacgacggg
      121 ctcgccgacc atcgaggaat cgctaggatc gttacaacta ctgtcaggta aatagcttct
      181 gccgacgacg cacgttcctt ctagaccccg tccgcgtgaa tccagccacc tgccgtgagt
      241 tcatctcggc ccgcttcggg tcttacgcct gatgcaagca cgcatcggtt aatcgcttgc
      301 gatgccggcg cagtgtggcg cggacctgga aattgggatc ctgggtgcga tcacgtaaac
      361 tgacttctcg aaggtcgtat ctgctccatt gtcactaagc gcggcgggtg gacagaaatc
      421 cgccttgatt caacaaattt gcccagcgag tgaccggtcc attgaccgca agagttccag
      481 tttggctcga tccaaagtat gcgaatgcag ccgaccatgc tacgacgtcc gtctctcatg
      541 gtccgcgcgg ccgcggcgca acttatcttg tttgtgggcc cggcgaagcc gaagttggcg
      601 gacggaaacc tccggccggg agcactggcg cccgctcgaa tccctcgggc aaagtgccgg
      661 tcaagctgcc gagaaaggtt acgatctcgt tgacctcttg gtcggagaga tcggcgtcaa
      721 gctggatctt cgccatgatg tggacggcct tcggcagagt gtcgaccgcg ccgtcgtgaa
      781 agtaaggagg cgtcatcgcg acgttgcgca gtccggcaac cttgaacttg tagaggtcag
      841 cgggctcgtt cgtcagcacg aatcggccct tgtcgatgtc ctggctgccg gtcaacggcc
      901 agtagtccga gacgacgcca aacttgcgaa acccgagtcc gccgagtccc gaccccttgt
      961 gacactcgac gcagccggtg tcgatgaacg tccgcaatcc cttacgctcc gccgcgctca
     1021 gggcgtcgga cttgccggcc agataatcgt cgaaccgcga tggagagacg agtgtccgct
     1081 cgtaggcgcc gatggccttg ccccagttat cttccgaaac tgggtcggcc tcgccgggga
     1141 aggcttgttg aaacatcgcg ccatagccgg gaattgcttt gaccctcgcc atcgcggtcg
     1201 ggaagtccgg attgccgaag cccggcccca gcaaggcgct cttcgcctgc gcctcgacgt
     1261 tggcgaaccg gccgtcccag tgctcgctgg tgtagagccc ggcgttgaac acggttgggg
     1321 cgttccgcgg aagaggtttg tcgtgcactc cgaccgactt gggcagtcca tccgcgccgt
     1381 aaagagcagg ttgatggcaa cgcatgcagc ttccggtgcc gtcggccgag atgcgcggat
     1441 cgaagaacag catgcgaccc aggagcacgc gttccggcgt gaccggaaac tccttggtcg
     1501 ccatgtcctt cggcagcggc tggaagaggc cacgggcgtc tttgagtaaa tcggcgtcgt
     1561 cgcttgcgga agcggatttt gcggcgagac cggccccgat cgcggaggcg acaaggcaaa
     1621 tgatccagac cggtttgtag tcagcaactt ggagcggttt catgtgaatc ctccggaatt
     1681 ccatcggtcg ccgatcagag cgacagaccg ttgcgaactt agatcttcaa tatacacctc
     1741 agccatttaa ttaacgcgga aagtatctga gggatccttc gtcgattttc gaatgagtct
     1801 tgttctcggc tcaagcccga ctgatttgag atcccttcta cgagcgcgga tctcactgcg
     1861 agttgttttc accccgggag ctttccaccc gtcgcagatc gcggctaaga tcctcgccga
     1921 gatttaccgc attgagctct gcggaaccgt cgagcttgat gagccaattg acgcacacgt
     1981 cgaaatgcac tcgtccctca tcgacccggc agtcaaggac gtgcccgccg accttgcgat
     2041 cgtcggagag gaagtgccag tggtagccgg ggacgttcag cccctcgacc cacgccgggc
     2101 atcggacgcc cacgagcgtc ccgcacacct cggcatgcgt ccagaccgac tgccccttga
     2161 ccacctcgcc caggggccg
//
LOCUS       DATAQL010000024         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3514113, whole genome shotgun sequence.
ACCESSION   DATAQL010000024 DATAQL010000000
VERSION     DATAQL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3514113"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /gene="rplM"
                     /locus_tag="VHS97_00420"
     CDS             <1..441
                     /gene="rplM"
                     /locus_tag="VHS97_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX3446685.1"
                     /translation="TYTPHIDTGDYVIVTNVDKVVFTGNKWRQKFYQRYSVYPGGQTD
                     EAAWKLFERQPERILELAIQRMMPKNKIGRHMMAKLKLYTGPNHPHQAQCPIPLEPMS
                     GRPTPAGALFVTTADATDSGSAGSENAATPHPADEVTPPVTSEV"
     gene            557..961
                     /gene="rpsI"
                     /locus_tag="VHS97_00425"
     CDS             557..961
                     /gene="rpsI"
                     /locus_tag="VHS97_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX3446686.1"
                     /translation="MTATAQKTYTWGTGRRKTAVARVRIREGTGQFIVNGLDCNEYFP
                     LIVQQKDTREPLHLTDMAGRIDVFVNVRGSGKLSQAGAVMLGLARALKDFRPDLEAKL
                     REHGMLTRDARMVERKKYGHKKARKSFQFSKR"
     gene            complement(1083..2102)
                     /locus_tag="VHS97_00430"
     CDS             complement(1083..2102)
                     /locus_tag="VHS97_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3446687.1"
                     /translation="MTETEPDRHVMTGQLVRIFKAPEWGLVAGIAVVLAVIYFLDRDR
                     AFFREVNIRPLLHRVALFGVLAIGAAVVIIAGGIDLSTGAVVALSSVVSAKLMTEWLR
                     RGGPSAPPPSVALVALAIGLTLLMGLMIGLVHALMINKLRIPPFIATLATMAGLRSLA
                     TILCENRTINVSFDAFRVLGKEAWVALSIFSGVAVLMSILMGWTVLGRHLFALGGNEM
                     AARLSGLRTNRLKAVAYGLSGTLSALGGILFTGNSGQADSRLGTTYELTAITAAVVGG
                     CSLSGGVGSIRGTVLGLLLTQIVLKATGIVVKGIDSTRVEGLVLGTVVLLAVAFNQRF
                     RGART"
     gene            complement(2163..3317)
                     /locus_tag="VHS97_00435"
     CDS             complement(2163..3317)
                     /locus_tag="VHS97_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3446688.1"
                     /translation="MDYAFSEAQQHWYEAATRFAREELIDAEAASREERGEFWREGYK
                     RCARFGILGLPVPTEYGGQGQDLATTVAAVEGLGYGCPDTGLVFGTGASIWTVTMPIL
                     AHGTDAQKRRYLPGLCDGSILGANAASEPEAGSDIFSMEMRAVRRGDGCWTLNGRKTW
                     ITSGAIADLLVCFASTDASKGVLGITAFLVERGTPGFHVKRRIENLGLRTAPMAELAF
                     EDCVVPADSILGREGRGSTLFNAALEWERGAILAGIVGTMRRQLDRCIERVRSRKQFG
                     QSIGKFQSVSNRIVDMSVRLETSRYMVYRYAWSKAHGKDAALWASMAKLHVSESFVQN
                     SLDAVRLFGASGYVSGEDQERDLRDSVGGVIFSGTNDIQRNLIAQYLRLG"
     gene            3598..3816
                     /locus_tag="VHS97_00440"
     CDS             3598..3816
                     /locus_tag="VHS97_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446689.1"
                     /translation="MSVKCLIVYYVNGQWIGEEVNWKRSGSGSYREVSRVEIPQSKAQ
                     IEAFAAENRFVIEWRGKLPAETPSAASA"
BASE COUNT          840 a         1197 c         1166 g          727 t
ORIGIN      
        1 acttacacac ctcacattga caccggtgac tatgtcatcg tgaccaatgt cgacaaggtt
       61 gtcttcacgg gtaacaagtg gcgtcagaag ttttaccagc gctattcagt ctatcccggc
      121 ggtcagacag atgaggccgc ctggaagctg tttgagcgtc agcccgaacg gatcctggag
      181 ctcgcgattc agcggatgat gcccaaaaac aagatcggcc gccacatgat ggccaagctc
      241 aagctttata ccgggcccaa tcatcctcac caggcccagt gcccgatccc gctcgagccc
      301 atgtcgggac gtccgacccc ggcgggcgcc ctattcgtca caacggcaga tgccacggat
      361 agcgggagcg cgggctcaga gaacgcggcg acccctcatc ccgccgatga ggttacgcca
      421 cccgtgacga gcgaagtctg agcaacgtat acagttcgtt ttgagcaaac tgagtgatac
      481 gaatcttttc gtcgagtacg gaacagttct ctcgcaataa agcgtaggac ttgtcccagc
      541 caggaaatca tcaaacatga cggcgacggc gcaaaaaaca tacacctggg gaaccggccg
      601 acggaaaacc gcggtcgccc gtgtccggat ccgtgagggg accggccagt tcatcgtcaa
      661 cggactggac tgcaacgagt acttcccttt gattgtccag cagaaagaca cgcgcgaacc
      721 gctgcacttg accgacatgg cgggacgtat cgatgtattc gtcaatgtcc gaggcagtgg
      781 caagcttagc caggcaggcg cggtcatgct cggtctggcc cgagcgctca aggattttcg
      841 ccccgacctc gaggcaaaac ttcgcgaaca cggcatgctg acccgcgatg cccgcatggt
      901 cgagcgcaaa aagtacggcc acaaaaaagc ccgcaagagt ttccaattct ccaagcggta
      961 ggtcgactct ggagcgtatt ccggcacaca accgaaaccg atccgcattg gtgctctggc
     1021 gaccgatgcg gatcggtttt ggttgtggcc gttctccaat gtcgcgtcct tatggccgag
     1081 ggctaagttc gcgcgcctcg gaaccgctga ttgaacgcca cggcgaggag taccaccgtt
     1141 cccaggacca gtccctcgac ccgggtgcta tcgattccct tgacgacgat gccggtcgcc
     1201 ttgagtacga tctgagtgag gagcagcccc aggaccgtcc cgcggatcga tccgacgccg
     1261 ccggaaaggc tacagccgcc gacgaccgcc gccgtgatgg cggtgagctc ataggtcgta
     1321 cccagtcggc tgtcggcctg gccgctattt cccgtaaata agatgccccc gagcgccgag
     1381 agggttcccg acaggccgta ggcaacggcc ttcaagcgat tggtccgaag gccgctcaac
     1441 cgcgcggcca tctcgtttcc tcccaacgca aagagatggc ggcccagaac ggtccatccc
     1501 atcaagatgc tcatgagcac ggccacgccc gagaagatcg aaagcgcgac ccaagcttct
     1561 ttgcccagca ccctgaatgc atcgaacgaa acgttgattg tcctgttctc gcagaggatg
     1621 gtggcaaggc tgcgcagacc ggccatcgtc gccagcgtcg cgatgaaggg tggaatccga
     1681 agcttattga tcatgagcgc atggaccaaa ccgatcatta gccccatgag cagggtcagc
     1741 ccgatggcga gcgcgacaag cgcgaccgac ggggggggag cgctcggtcc acccctacgt
     1801 agccactcgg tcatgagctt ggcgcttacc accgacgaga gtgcgacaac agccccggtc
     1861 gacagatcga ttccgccggc gatgatcacc actgctgcac caatcgcgag cacgccgaaa
     1921 agcgcgacgc ggtgcaacag cggccgaatg ttcacttccc gaaaaaaagc gcgatcgcga
     1981 tcgagaaagt agatgacagc caggacgacg gcaatgccgg caactagtcc ccattcgggg
     2041 gccttgaaaa tacgtacgag ctgtccggtc atcacgtgtc ggtcgggttc ggtttcggtc
     2101 atcatggtac acgagtgttg aagacggctt cgctgccgcc gagtggcgat cgaccgacga
     2161 gtttaaccca gccgcagata ctgtgcgatc aggttgcgct gaatgtcatt agtgccggag
     2221 aagattacac cgccgacgct atcgcgcaga tcgcgctctt ggtcctcgcc ggagacatag
     2281 ccagaggcgc caaagaggcg aaccgcatcg aggctgttct ggacgaagct ctctgatacg
     2341 tggagcttgg ccatcgatgc ccagagcgcg gcgtccttgc cgtgtgcctt ggaccaagcg
     2401 tatcgataga ccatgtaccg gctggtttcc agccggacgg acatatcgac gatccgattc
     2461 gagacagact gaaacttgcc gatcgattgg ccgaactgct tcctggatcg cacccgctcg
     2521 atgcagcgat cgagttgtcg gcgcatcgtg cccacaatcc cagccaaaat cgcaccccgt
     2581 tcccattcga gggcggcatt gaagagggtt gagcctcgtc cttctcggcc aaggatactg
     2641 tcggccggca ctacgcagtc ctcgaaggcg agttcggcca taggcgcggt gcgtaaaccg
     2701 aggttttcaa tgcggcgttt gacgtgaaat ccaggagttc cgcgctccac gaggaacgcc
     2761 gtaatgccga gtaccccctt ggaggcatcg gtgctggcga agcaaaccag gaggtcggcg
     2821 atggcgccgc tcgtgatcca ggtcttgcga ccgttcaagg tccagcagcc gtcaccgcgg
     2881 cggaccgcgc gcatttccat gctgaagata tcggaccctg cttcgggttc actggccgca
     2941 ttggccccca ggatactgcc gtcgcagagg ccgggcaggt agcgtctctt ctgagcgtcg
     3001 gtgccatgtg ccaggatcgg catggtgacc gtccagatcg aggcacccgt cccgaagacc
     3061 aatcccgtgt cgggacagcc gtatcccagc ccttccacgg ccgcgacggt cgtcgcgaga
     3121 tcttgaccct gaccgccata ctccgtcggc accggcaaac cgaggatgcc gaaccgcgcg
     3181 cacctcttgt atccttcgcg ccagaactcg ccgcgctctt ctcggctcgc agcttccgcg
     3241 tcgatcaact cttcccgagc gaaccgcgtg gctgcctcgt accaatgttg ttgtgcttcc
     3301 gaaaaggcat aatccatcac cgcgtccatc tcgtatgatg ttgtaggtcg ccttgttccc
     3361 cgatcgacga gaaccgtgag cgctctcggc gccgacggaa cagcctttcg caggccggca
     3421 attgccggca atgatgatgg ccaaagactc tattttgggc aagacgttaa cagctcggat
     3481 gccagtcgag tgtatcgttg cggcgcgatg ccgaatcgaa gccgcgtctt ccgcttggcg
     3541 agaacgcacc gctggtttat cattttttgg ggatgatttt ctggctcact cggagacatg
     3601 agcgtgaagt gtctgatcgt ctactacgtc aacgggcaat ggatcggtga ggaagtgaac
     3661 tggaaacgct cgggaagcgg gagctatcgc gaggtctcgc gcgttgagat tccccaatcg
     3721 aaagctcaga tcgaggcgtt cgcggctgaa aaccggttcg tgatcgagtg gcgcggcaag
     3781 ctgccggcag aaacaccgtc cgcggcgagt gcgtgagcga cgggagaaag caaccgagtg
     3841 aagcaagaaa cgacctcggc ggacgttgag ttgaagggca gtgtggcttg atgtccattg
     3901 acaccggcgg ccctccactt cgcctggccg
//
LOCUS       DATAQL010000025         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2292690, whole genome shotgun sequence.
ACCESSION   DATAQL010000025 DATAQL010000000
VERSION     DATAQL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292690"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VHS97_00445"
     CDS             <1..820
                     /locus_tag="VHS97_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446690.1"
                     /translation="TAAIRLAVIVTFVLFHIVGLGMCIELGLFPHTCVVGWLALTPGQ
                     FWDALNRRWRVSRSSARAVDRGTWVHAMRAPCAETIPDSEAPADDVGLPLNMNVLAGF
                     FLVYVLLWNIRTLDYQKYHRFLPAKLDWIGHTFKLNQCWDMFAPFPSKEGGWHVMRGT
                     LRDGTEVDLWRGGAPIRLEEPELVSAIYRNSRWRKYLTNLNYEEYARYRENFARWLCR
                     DWNARHVEARRLAEVEIDYMRKVTRLDHDAAAPEKVLIIKHSCLDTCTGGGSGD"
     gene            863..1159
                     /locus_tag="VHS97_00450"
     CDS             863..1159
                     /locus_tag="VHS97_00450"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX3446691.1"
                     /translation="MITVQGRSVDELKTAFQDSIDDYLASCKQRGEEPNTPFSGEFVT
                     RIPPELHRQINLAASISGKSLNVWVDEQLVAAVTRIESMKAAKATGKGMSSMIP"
     gene            complement(1624..1706)
                     /locus_tag="VHS97_00455"
     tRNA            complement(1624..1706)
                     /locus_tag="VHS97_00455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Leu,seq:caa)
     gene            complement(1796..2137)
                     /locus_tag="VHS97_00460"
     CDS             complement(1796..2137)
                     /locus_tag="VHS97_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446692.1"
                     /translation="MAKNPIPIAHDVSPVRPNSSPRRVGMMCLLAIIIVPIVVESAAL
                     SYGQWCSITGRSTRVSTPIMDTVSNAFHETRSVVEDVFSPSFARVFREPGVAIPVALV
                     LLGIGMTLLRR"
     gene            complement(2218..>2483)
                     /locus_tag="VHS97_00465"
     CDS             complement(2218..>2483)
                     /locus_tag="VHS97_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3446693.1"
                     /translation="GIRTGVDDVRSLAAVIIQAETFGSSISAALRVQSDAMRLRRRQM
                     AEERAAQTSVKIMIPLIFFIFPGVFVVLVGPAVMRMMSAFGSP"
BASE COUNT          502 a          749 c          744 g          488 t
ORIGIN      
        1 cacggcggcg attcgcctcg ccgtcatcgt gaccttcgta cttttccaca tcgtcggcct
       61 cggcatgtgc atcgagttgg gcctctttcc gcacacctgc gtcgttggct ggctggcctt
      121 gacgcccggc cagttctggg atgcgttgaa ccgccgctgg cgtgtgtccc ggtcgtcggc
      181 tcgggccgtt gaccgaggca cgtgggtcca cgccatgcgc gctccgtgcg ccgagacgat
      241 cccggacagc gaggctcccg cggacgacgt tggcctcccg ctgaatatga acgtgttggc
      301 cggcttcttc ctcgtatatg tcctactctg gaacatccga accctggatt atcagaaata
      361 ccaccgattc ctcccggcga aacttgactg gatcggccac acattcaagc tcaaccagtg
      421 ctgggacatg ttcgctccgt ttccttcgaa ggagggcggc tggcacgtga tgcgaggtac
      481 gttacgcgac gggaccgagg tggacctctg gcgaggcggc gcgcccatcc gcttggaaga
      541 gcccgaattg gtgtcggcga tctaccggaa cagccgctgg cgaaagtatt tgacgaacct
      601 gaactatgaa gaatacgcgc ggtatcgcga gaattttgcc cggtggttgt gccgcgactg
      661 gaacgcccgg catgtcgaag cccggaggtt ggccgaagtc gaaatcgatt acatgcgcaa
      721 ggtaacgcgg ctggaccatg acgcggccgc ccccgaaaaa gtcctcatca tcaagcattc
      781 ctgtctcgac acctgcaccg gcggaggctc aggggactga ctcgcgggga tcttccacgg
      841 cgaggtcctc gacacgcggg acgtgatcac cgtccaggga cgaagcgttg acgagttgaa
      901 gaccgccttc caggattcga ttgacgatta tctcgcctct tgcaagcagc gtggagaaga
      961 gccgaacaca ccgttctcgg gcgagttcgt tacccgcatt ccgccggagc ttcatcgcca
     1021 gatcaatctg gcggcaagca tttccggcaa gagcctcaat gtctgggtcg acgagcagtt
     1081 ggtggcggcc gtcacccgca tcgagtcgat gaaggcggca aaggccacgg ggaaaggaat
     1141 gtcgtcgatg atcccctgat cgactttggc cgccgccggg caagagatga aggaacgcac
     1201 tgatcaaaac gggcaagatt gactgggtcg ccctcgcaac cggacgccgt ccaggtatcg
     1261 ttgtctcgcg gcaaagcctg aaccaaggaa accagattgt cgttgtcctt tgcacgacga
     1321 accgtttggc catccgttcc aagctgccga attgcgtacc gttccatgcg ggtgacttcg
     1381 gcatcccggc cgatcgtgtg gcacagtgtg aggcgatcta tgccttgggc gagggggaga
     1441 ttgctaccgc gtctggcgtg attggccggc tcgatgctgc acgataggga gacgtcatca
     1501 aggccatcgg caacgtgatc gactcggact gcgaactgaa ctgagctaat ggccgctgca
     1561 cgcgcgaatc tgggtagggc gatgaggccg ctttggactg ccctctgccg cttcgctctt
     1621 gagtaccgaa ggtgggactt gaacccacac cctcttgcga ggaccggatt ttgagtccgg
     1681 cgcgtctgcc attccgccac ttcggctcgc atattctgtt ttaggatcct ataagagcca
     1741 aaccgctgtc aaccccgatg ggctgctgct cttaaggatc gactcaaacc ctttgctatc
     1801 gcctcagcag cgtcattcca atgccgagga ggaccagcgc gaccggaatc gcgacaccgg
     1861 gctccctgaa cacgcgcgcg aaactgggcg aaaagacatc ctcaacgaca gatcttgtct
     1921 catgaaaggc gttcgagact gtgtccatga tgggagtgct cacccgggtt gatctacccg
     1981 tgatgctgca ccattggccg tacgagagcg ccgcactttc tacaacgatc ggcacgatga
     2041 taatcgccaa caagcacatc ataccgactc gccgcgggct cgagttcggc cgcaccgggc
     2101 tgacgtcatg agctatcggg atcgggttct tggccatcgc ggttctccgg gcaaacaaag
     2161 ggtgtgaaaa atgttgtgtc ggcgtcggca tccgaaatca gtgcgtcttt cggtacttta
     2221 gggagaccca aatgcgctca tcatgcgcat caccgcgggc cccaccagga ccacaaacac
     2281 acccgggaaa ataaagaaga tcagcgggat catgatcttg acggaagtct gggccgcgcg
     2341 ttcctccgcc atctggcggc ggcgcagtcg catggcgtcg gattgaaccc gcaacgcagc
     2401 cgaaatgctc gagccgaacg tctcggcttg aatgatcacc gcggcgagtg accgcacatc
     2461 gtcgacgccc gtccggatcc cca
//
LOCUS       DATAQL010000026         4777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2139999, whole genome shotgun sequence.
ACCESSION   DATAQL010000026 DATAQL010000000
VERSION     DATAQL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139999"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VHS97_00470"
     CDS             complement(<1..1161)
                     /locus_tag="VHS97_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX3446694.1"
                     /translation="MRGYTRFAILALILYLDSGLASAKSNAAPGSRPNIVVIMADDMG
                     YGDLGAHGNPKIKTPNLDRFASQSVRLKNFYVSPVCAPTRASLLTGRYNFRTGVVDTY
                     LGRALMRSEEVTLAEMLAGAGYRTGIFGKWHLGDNAPLRPMDQGFERSLVIKGGGIGQ
                     PSDPPGGSSYFDPILQTDSRSQRFKGYCSDIFAQAAIDFLNQPSDHPFFAYLAFNCPH
                     EPLEAPEADLAVYRAMKLELGEFPQLGQPVPPNFAASADTVARVYAMITNIDTNVGKV
                     LHSLDARGLADNTIVVFLTDNGPAQVRFNAGLRGWKGSVFDGGIHVPCYIRWPGRFPA
                     GRVVDPIAAHIDLAPTLLDACGVPPPAGVQFDGKSLIPLLKGFQSAGWPERTL"
     gene            1650..3272
                     /locus_tag="VHS97_00475"
     CDS             1650..3272
                     /locus_tag="VHS97_00475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3446695.1"
                     /translation="MSGLDALEGRQLMTASLQAIPTVTVPALQGYTVPLLANSGATDA
                     QTFTVTSSNPNITASVAQGPFWTLGVASKDSANPSASFTGSLTFQLFQNLTPNTVKMI
                     EQFTNDGFYVNSGDFFPRVVSGFDSPQTTVIQGGATNVNGTGSSGQPNTPFANENVQQ
                     LALTGVDQLALANAGGTDTNNTQFFINTGPADALGYNYTVFGQLVAGGATLAQMAHVP
                     VQTNSVTGENSQPVNPLTITSASLSSTSPDGVVILNTTQAKQGETATITVTATDPTDG
                     TKASQSFNVEVGPYTGPTTSTAIGNVNFKPFANPVSTTAYENAATQIQLSGQSTYPDA
                     TAKAPLNYTLTTLPSHGSVSNFNASTGTFTYTPQSGYLGNDTFQYLVTANGPNTAAAL
                     ATSNPAMVTVGVSAAPPVVSVQNVALSTNRRHMVTQIDVTFSGQVNATQADNTGIYQL
                     AYPDKSGSYTGKNAVVVKLHSANYSAATNSVTLTPRKPFALKARPLQLVIDGTSPSGL
                     QDIVGRYLDGADNGQAGSDAVVSISKNGAKIG"
     gene            complement(3285..4730)
                     /locus_tag="VHS97_00480"
     CDS             complement(3285..4730)
                     /locus_tag="VHS97_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3446696.1"
                     /translation="MSHLIPRRDVLWRIGGGLGGIALATMLGEEGLLASTELTPQSPR
                     PRADWNGGLHHRAQVRRVIQLFMNGGVSQMDTFDYKPELHRGHGQTFDPGGHVEAPTS
                     APGKLMQSPFKFRQYGDCGRWVSSVFPEIAACVDDLTFLMALSSKTNVHGPASFMMNT
                     GFLMPGFPAMGAWLSYGLGSIADNLPTFVVLPDPKGLPYNAKGNFTAGFLPQSHQGVI
                     LDAGAPRPIPELFPPASASYLTPESQREGRAVLDQMNRQHAAANPGDTRLEARIAAYE
                     LAARMQQSAPEALDLAGESAATRTLYGVDDPSTADYGRRCLLARRLLERGTRFVQVWS
                     GAGGPINNWDNHNDIPNQLPPMARSVDRPIAGLLRDLKGRGLLEDTLVVFSTEFGRQP
                     FTQGATGRDHNGGTSVAWLAGAGVKAGASFGESDPWAWRAATGQTYCYDLHATILHLM
                     GIDHTKLTFRHNGIDRRLTDVHGEVIHAILA"
BASE COUNT          971 a         1494 c         1433 g          879 t
ORIGIN      
        1 tagagtccgt tcgggccagc cggcgctctg aaatcctttc aagagcggga tcaagctctt
       61 gccgtcgaat tggacgccgg ccggcggcgg cacgccgcag gcatcgagca acgtcggcgc
      121 caggtcgata tgagcggcga ttgggtccac gactcgcccg gccgggaagc gcccaggcca
      181 acggatatag cagggaacat gaatgccgcc gtcgaacacc gaccccttcc acccgcggag
      241 gccggcgtta aatcgcacct gggctgggcc gttatcggtg aggaaaacaa cgatggtgtt
      301 gtcggctagc ccgcgcgcgt cgagtgaatg taagaccttg cccacgtttg tgtcgatgtt
      361 ggtgatcatg gcgtagaccc gcgcgaccgt gtcagcagac gccgcgaaat tcggcggaac
      421 tggttgcccg agctgaggga attcgccgag ctcgagcttc atcgcccggt aaaccgccag
      481 gtcggcctct ggagcctcga gcggttcgtg cggacaattg aaagcgaggt aagcgaaaaa
      541 gggatgatcg gaaggctgat tgagaaaatc gatggcggct tgggcgaaga tgtcgctgca
      601 atagcccttg aagcgctgcg atcgactgtc agtctgcaag atcgggtcga aatagctgct
      661 gccgcctggc gggtccgacg gttggccgat accgcctccc ttgatgacga gcgacctctc
      721 gaacccctga tccatcggcc gcaggggcgc gttgtccccc aaatgccatt tgccgaagat
      781 gccggtccga tagcccgcgc ccgccagcat ttcggccaac gtgacctctt cagagcgcat
      841 cagggctcgg cccaagtagg tatcgacaac gccggtgcgg aaattgtagc ggccggtgag
      901 caggctggct cgcgtcggcg cacagaccgg tgacacataa aagttcttca gtcgcacgct
      961 ctggctggcg aaacggtcaa ggttcggcgt cttgatcttg gggttgccgt gtgcccccaa
     1021 gtcgccgtag cccatgtcgt cggccatgat gactacgatg ttgggccgcg acccgggcgc
     1081 cgcattggat tttgcgcttg ccagaccact gtccaaataa agaatcagcg cgagtatcgc
     1141 gaaacgcgtg tagcctcgca tggaaccatc ctggctgaac tgtcgacgat gcctgaaact
     1201 ctgcattccc agaatacctc gctgctgcta gactcgccag aacgggatct gaaccctgcg
     1261 gttccgccgg gttttctatc gatattcatg tcggtcttcc tttcgccaag tacatgtcag
     1321 agcgatctaa gtggacccgc cgaagtcgcc gcgcagcgta ggtcgggctt tctagcccga
     1381 cgttttcctt gaacgcacaa aatgtcgggc tggaaagcct gacctacaca agtagttctg
     1441 cagtctcact tcggccgtga tcacgtcact tcttagcagc ggaccgtcgc gccaagtcgg
     1501 atgaatggtt ttggttagag cgcgacgcgt cgcagaaaac catgagccat ggaaggtgat
     1561 ttatgtggga cagtagcctc ggacgccgct cccaaaccgc ccggccattg accggccaaa
     1621 acgccagacc ggtacgtcga atttcatgtt tgtccgggct cgacgcgctg gaaggccggc
     1681 agttgatgac agcttcgctg caagcgattc caaccgtgac tgtccccgcc ctccaaggct
     1741 ataccgtccc cttgctcgcg aacagcggcg ccaccgacgc ccagaccttc acggtcacgt
     1801 ccagcaatcc gaacatcacc gcatcggtgg cccaaggccc gttctggacc ctgggagtcg
     1861 cctcgaagga ttcggcgaat ccatccgcca gcttcacggg ctcgttgacg tttcagctct
     1921 ttcaaaactt gacgccaaac accgtcaaaa tgatcgagca gttcacgaac gacggcttct
     1981 acgtgaactc cggtgacttt tttccgcggg tcgtgagcgg ctttgactcc ccgcagacga
     2041 cagtcattca gggaggtgcg accaacgtta acggcaccgg ttcgagcggt cagccgaata
     2101 cccccttcgc caatgaaaat gtccagcaac tggcactcac gggcgtggat caattggcgc
     2161 tggccaacgc gggagggacg gacacgaata atacacagtt tttcattaac accggcccgg
     2221 cggatgccct gggatacaac tacacggtct ttggtcagct tgtcgccgga ggagccactc
     2281 tggcgcagat ggctcacgtt ccggtccaga cgaactcagt gactggcgaa aactcgcagc
     2341 cggtcaaccc cctcaccatc acctccgcgt cgctgtcgtc cacgagtccc gacggcgtcg
     2401 tgatcctcaa caccacgcag gcgaaacagg gcgagacggc caccatcacc gtcacagcca
     2461 ccgacccaac cgacggtacg aaagcttcgc agtcgttcaa cgtcgaggtc ggtccctaca
     2521 cagggccgac gacctccact gcaatcggca atgtcaattt caagccgttt gcgaatccgg
     2581 tctcgaccac ggcgtatgaa aacgcggcga ctcagatcca gttgtcgggt caaagcacct
     2641 accccgacgc caccgccaag gcaccgctaa actataccct gaccacgttg cccagccatg
     2701 ggagcgtcag caatttcaac gcatcgacgg gcacgtttac ctacacacct caatcgggtt
     2761 atctgggtaa cgacacgttt cagtatctgg tgacggcgaa cggtcctaat acagccgccg
     2821 ccctggcgac cagcaacccg gccatggtga ccgtcggcgt ctcagcggcg ccgccggtgg
     2881 tatcggtgca gaatgtcgcg ctttccacga acagaaggca catggtgacc cagatcgacg
     2941 tcacattcag tggtcaggtc aacgccactc aagcggacaa cacagggatc tatcaactgg
     3001 cttatcctga caagtcaggg tcttacaccg gcaagaacgc ggtcgtcgtc aaactgcact
     3061 cggcgaacta cagtgcggcg accaacagcg ttaccctcac tccgaggaag ccgtttgcgc
     3121 tcaaggcgcg accattgcaa ctcgtcatcg acggaacgtc gccatccgga ctccaagaca
     3181 tcgtgggccg ctatctcgac ggagcggaca acggccaggc ggggagcgac gccgtcgtct
     3241 cgatctccaa gaatggcgcg aagatcgggt gatgcggtac ggattcacgc caggatcgcg
     3301 tggatcacct ccccatgaac atcggtcagg cggcggtcga tgccgttgtg ccggaatgtg
     3361 agcttggtgt ggtcgatccc catcaggtgg aggatcgtgg cgtggaggtc atagcagtaa
     3421 gtctggccgg tggcggctct ccatgcccag gggtcgcttt cgccaaaact cgcgcccgcc
     3481 ttcacgcccg cgcccgcgag ccaggccacc gacgtgccgc cgttgtggtc gcggccggtg
     3541 gccccttgag taaacggctg gcggccaaat tcggtgctga agacgacgag cgtgtcttcc
     3601 agcaggccgc ggcccttgag gtcgcgcaat aaacccgcga tcggccggtc gacgcttcgg
     3661 gccatcggag gcaactggtt gggaatgtca ttgtggttgt cccagttgtt gatcggcccg
     3721 cctgcgccgc tccagacctg gacgaagcgc gtgccgcgct ccaacaaccg gcgggccagc
     3781 aagcaccggc ggccatagtc ggccgtggaa gggtcgtcga caccatagag cgtgcgcgtg
     3841 gcggcgctct ctccggccag gtcgagtgct tcgggagcgc tttgctgcat ccgagccgcc
     3901 agttcatagg cggcgatgcg cgcttcgagc cgcgtgtcac cagggttggc ggccgcgtgc
     3961 tggcggttca tctgatcgag gaccgctcgg ccttcacgct ggctctccgg tgtgagatag
     4021 ctcgcggatg cagggggaaa caactcaggg attggccgcg gtgcgcccgc gtcgagaatg
     4081 accccttggt gtgactgcgg caagaaaccc gctgtaaaat tacccttggc gttgtacgga
     4141 aggcccttgg gatcgggcaa gaccacaaaa gtcggcaagt tgtcggcgat cgagcccaag
     4201 ccgtaggaaa gccacgcccc catcgcggga aagccgggca tcaagaagcc ggtattcatc
     4261 atgaagctgg ccggtccgtg gacgttcgtc ttggagctca gggccatcag aaacgtcagg
     4321 tcgtcgacgc aggccgcgat ctcgggaaac acgctgctga cccagcgccc gcaatcccca
     4381 tattggcgga acttgaacgg gctctgcatc agtttgcccg gcgcgctcgt cggcgcctcg
     4441 acgtgccccc cagggtcaaa cgtttggccg tgaccccggt gaagctcggg cttgtagtca
     4501 aaggtgtcca tctggctcac gccgccgttc atgaagagct ggatcacccg gcgaacctgg
     4561 gcacgatggt gcagccctcc gttccaatcc gcgcgcgggc gtggtgattg cggcgtcaat
     4621 tcagtactgg cgagcaagcc ctcctcgccc agcatcgtcg ccagcgcgat tcccccaagt
     4681 ccgccgccga tccgccagag aacatcgcgc cgggggatca agtgagacat gacgaatcgc
     4741 ctcatcaatt tacgaacaga aactcgttac tattgaa
//
LOCUS       DATAQL010000027         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466 k141_3387,
            whole genome shotgun sequence.
ACCESSION   DATAQL010000027 DATAQL010000000
VERSION     DATAQL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3387"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2542
                     /locus_tag="VHS97_00485"
     CDS             <1..2542
                     /locus_tag="VHS97_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3446697.1"
                     /translation="LDPAKVARSAAIEATKLDDIRKVVVDTSDDGTPIYVRQIAQVVI
                     GYQPRLGIVGRGAEDDVVEGIVLMRKYEKSLPTAEAVAAKMEEIERERLLPKGMTLRV
                     FNQRTDLVDVTTHNVLHNLVEGMFLVIIVLFVFLGDILSAGIVAIMIPLALLFSVTVL
                     YVQGKSANLLSIGAVDFGIIVDSSVIIVENIYRHITAHDADRSRPFIERIIEASYEIE
                     RSLFFSTTIIVCAFIPLFAMTGPEGALFGPMASTYAFAIFGALLLALTLAPVLCSFLL
                     VNKTEETETIVDRMMKRRYLKNLDRVLDHRFTTLMLMCGLLVFTVSLVPSLGGEFMPQ
                     LEEGNLWIRAIMPRTTSLAESARIAPRLREVMAAIPEVRGVMSQVGRPDDGTDITSFF
                     NLEFNVPLKPMEQWRKRTRTIFGHPVSFLWGGQDVLARTITREEVQDEMMDRFHEQFP
                     ALDFSFSQLIRDNVEEALSGVKGANSVKLFGSDLDVLENNGEKIANILRSVRGIQNVG
                     VFHIVGQPNFEIEIDREACARYGVNVADVESNVKVAIGGEAFSQMVEGEKLYDIVLRL
                     PYDLRDDPGDIAHLAVDVPGPKNKPGARIPLSHLATIDPHKPGASYIYRENNRRYLPI
                     KFAVQGRDLASAIGEARAKVKDPKYGAVLPPGYRLEWSGEFAQMEKANARLMWIVPIS
                     IGLIMVLLYTAFSSLKDALLVMVNVVAATMGGVWALQLTSTPFSISAAVGFISIFGVA
                     VQDGVLLISYFNQMRQSGLPVREAVMRGAELRVRPVVMTSLTAALGLLPAAMANSIGS
                     QAQKPLAIVVVGGMLVTLFLTRYLMPVLYTFFPAPGNLRIDYPNKVNS"
     gene            2539..>3030
                     /locus_tag="VHS97_00490"
     CDS             2539..>3030
                     /locus_tag="VHS97_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446698.1"
                     /translation="MIPTEKSATSTHAPNSIDGTAPTNGIVPHHASPTRESVSGRNFP
                     GPPTRRLVRFTVLMLTIGGIAAVAAWKFPQPMRDAMAALTGPKTTEPLARHVQFQPPA
                     VSPDGIITLGEKAVAGMGLEWAKAVSQTEPIRLELLGTTEYDSDNQTRIRPLFRGRVD
                     RVYA"
BASE COUNT          589 a          909 c          892 g          640 t
ORIGIN      
        1 actcgatccg gccaaagtcg cccgctccgc cgcgatcgag gccaccaagc tggatgacat
       61 tcgcaaggtg gttgtcgaca cctcggacga cggcacgccc atttatgtcc ggcagatcgc
      121 ccaggtcgtc attggatacc aacctcggct ggggatcgta ggccgcgggg ccgaagacga
      181 cgttgtcgag ggcatcgtcc tcatgcgcaa atatgaaaag tcgctaccaa ccgcggaggc
      241 ggtcgcggcg aaaatggagg agatcgagcg cgagcgtttg ctgccgaagg ggatgacgct
      301 gcgggtcttt aaccaacgca cggacttagt cgatgtcacg acccacaacg tgctccacaa
      361 cctcgtggaa ggcatgttcc tggtgatcat cgttctgttc gtctttctgg gcgacatctt
      421 gagcgccggc atcgtggcga ttatgatccc gctggctctc ttgttttcgg tcactgtgct
      481 gtatgtccag ggcaagtcag ccaatctcct gtcgatcgga gcggtcgact tcgggatcat
      541 tgtcgacagc tcggtgatca tcgtcgagaa catttaccgg cacatcacgg cgcacgatgc
      601 cgaccgatcc cggccgttca tcgagcggat cattgaggct tcctacgaga tcgagcgctc
      661 gttattcttc tcgaccacga tcatcgtctg cgcattcatt ccactgtttg cgatgaccgg
      721 gccagaaggg gccttgttcg gtccgatggc cagcacctac gcattcgcga tcttcggcgc
      781 cttgctgctg gccctcacgt tggccccggt cttgtgttca tttctcttgg tgaacaagac
      841 cgaggagacc gagacaattg tcgaccggat gatgaagcgg cgctatctga aaaacctgga
      901 ccgcgtgctc gatcatcgtt tcacgacact gatgcttatg tgtggcctgt tggtcttcac
      961 ggtgagttta gtgccgtcgc ttggtggcga gttcatgccg caactcgagg aggggaacct
     1021 ctggatccgg gcgatcatgc cccgcacaac ctcgctggcg gagtcggccc gaattgctcc
     1081 caggctccgc gaggtgatgg ccgcgatacc cgaggttcgc ggcgtgatgt cgcaggtggg
     1141 ccggcccgac gacggcacag atatcaccag ttttttcaac ctggagttca acgtcccgtt
     1201 gaagccgatg gaacagtggc ggaaaagaac caggacaatc tttgggcatc ccgtctcgtt
     1261 cctgtggggc ggccaggacg ttctcgctcg cacgatcacg cgcgaggagg ttcaggacga
     1321 gatgatggac cgattccatg agcaattccc ggctcttgat ttcagctttt cgcagctcat
     1381 tcgcgacaac gtcgaagaag cactttcggg cgtgaaggga gccaactcgg ttaagctttt
     1441 cggctccgac ttggacgtcc tggaaaataa cggggagaag atcgccaaca ttctgcgctc
     1501 ggtacgtgga atccagaatg tcggcgtctt tcacatcgtc ggccagccca atttcgagat
     1561 cgagatcgac cgtgaggcct gcgcccggta cggtgtcaac gtggcggatg tcgagtccaa
     1621 tgtcaaggta gcgatcggcg gcgaggcgtt ttcgcagatg gttgagggag agaagctcta
     1681 cgacatcgtc ctgaggctac cgtacgacct gcgcgacgat cctggggaca ttgctcatct
     1741 cgcggtcgat gtacccggcc ccaaaaacaa gcccggcgct cgcatcccgc tctcgcacct
     1801 cgccacgatc gaccctcaca agccgggtgc gtcatacatc taccgcgaaa acaaccgacg
     1861 ctacctgccg atcaagttcg ccgtccaagg ccgagacctc gcctccgcga tcggtgaggc
     1921 gcgcgccaaa gtgaaggacc cgaagtacgg cgccgtgttg ccaccaggat accggctcga
     1981 gtggtcgggt gagtttgccc agatggaaaa ggccaacgcc agactcatgt ggatcgtccc
     2041 gatctcgatc ggcctgatca tggtcctttt atacaccgcg ttcagttcat tgaaagacgc
     2101 cctgcttgtc atggtcaatg tcgtcgcggc gacgatgggg ggcgtatggg ctttgcagct
     2161 gacgagcaca ccgttctcga tctcggcagc tgtggggttc atctcgatct ttggcgtcgc
     2221 cgtccaggac ggcgtgctcc tgatctccta tttcaatcag atgcgtcaaa gcggcctgcc
     2281 ggtgcgagaa gcggtgatgc gcggggccga gctgcgggta cggcccgtgg tcatgacttc
     2341 tctgaccgcg gcactcgggc tacttcccgc cgcgatggcc aactcaatcg gctctcaagc
     2401 ccagaaaccg ctggcgattg tcgtggtcgg tggaatgctc gtcactcttt ttctgacccg
     2461 ctacttgatg cccgtgcttt acaccttctt cccagctcca ggcaatctgc gtattgacta
     2521 tccgaacaag gttaattcat gatcccgacc gaaaaatcag cgacatcgac gcatgcgccg
     2581 aactcgattg atggcaccgc tcccacgaat gggattgtgc cgcaccatgc gtcccccact
     2641 cgtgaaagcg tctcgggcag gaactttccc ggcccgccaa ctcgtcggct ggtcaggttt
     2701 accgtactca tgttgacgat cgggggtatc gcagccgtag ccgcgtggaa atttccccag
     2761 cccatgcgcg acgccatggc ggccctcacc ggccccaaaa cgaccgagcc cctggcgcgg
     2821 cacgttcaat tccagcctcc cgccgtgtca cccgacggca tcatcacact gggagaaaag
     2881 gcggtggcgg ggatgggttt ggagtgggcc aaggccgttt cccagacaga accgattcgc
     2941 ctcgagctgc tcggcacaac cgaatatgac tccgacaatc agacaaggat tcgtccgctt
     3001 ttcagggggc gagtggatcg cgtttatgcg
//
LOCUS       DATAQL010000028         4395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_461361, whole genome shotgun sequence.
ACCESSION   DATAQL010000028 DATAQL010000000
VERSION     DATAQL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461361"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VHS97_00495"
     CDS             complement(<1..184)
                     /locus_tag="VHS97_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446699.1"
                     /translation="MSARESPWFERWFGFLAFVGRTCARAPSAIVYRLPEVIRQFEQV
                     AVKSLPIVLGAGLSVGL"
     gene            537..2342
                     /locus_tag="VHS97_00500"
     CDS             537..2342
                     /locus_tag="VHS97_00500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3446700.1"
                     /translation="MSNASMERQDGLEVGASPWEIFALGALAPLAPYAQVHLAPKIPP
                     GLLNTALMSYLSLQSDEQLLAVIDGSGGRLAGSCALTTRRIYWAAVDDSDESQPTTKG
                     RLRVRVHRQLLCRHVLEYDTLPPAVIESRGPNGSFRLDFGGGRALNLKGVDANLALSL
                     RQYLDKIRAVARNESAPELAEMEPELAARVARAWPAVAQVSSRARSLGVDLRQFRRSL
                     ETATPHAFVTPLLIVVCVIVYGVMVVSGVSPLWPTGAQLILWGANEGARIILGHEYWR
                     LITSVFVHGGVIHLALNMWSLLAIGPLVERLYGNAAFAVIYLAAGIGGAIASVAASPV
                     RVSVGASGAICGVLGALLAFLLVHRRAIPTLVLKPLRANALGYVVFIAILGAVIPNID
                     QEAHLGGLAAGFVVGFLLSRPWPAVKSKWVAVRRAVVTVLVAAALAGAAAAIAGKGAM
                     TLPPQLRLQHLEQQIAPPLDRFNAIAKATPGSLVLRRDLLVPDDHQTHVRTIHELTEG
                     ANANLTILRRVKTPDPALRKLVSSLIEAQSSQIAMLAAARRFLETRNADELTGEAGFL
                     AEKAATDRSVRAFQEQQVHYLTERGLISKSVTKER"
     gene            2593..3972
                     /locus_tag="VHS97_00505"
     CDS             2593..3972
                     /locus_tag="VHS97_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HEX3446701.1"
                     /translation="MPIFSRTKSVTKGDPIVMAEHRRMSKFCLPIVMLTAGLTLAASP
                     RAANAGLDEYVKRPDGAFTWSKINKLSTPAGTITTLGLTSQVWQEITWKHELTIYEPR
                     ELSYTDAMLLFITGGNHNSKQNDNDHKQGFGLAQLCGARIAVLRQVPNQPLLDGKTED
                     ELIAETFVRYLQTKDENWPLLFPMVKSAVRAMDALQAFATETGKPVTRFVVTGGSKRG
                     WTTWLTGAVDDRVVAIAPMVIVTLNMGKQGPNQLKVWGKYSEQIGDYVERGLMEKADT
                     PDGTKLWKMVDPFTYRDRLTKPKLLINGTNDRYWTLDALDLYWNGLEGPKYLIELPNA
                     GHGLDANRDWALGGIGAFFRHVVTDRPMPKISWDMARGAGGEATLTIHADPAPQSARI
                     WTARSHSRDFRESHWESAPLKPGETITRYVPAEKSGNLAFFGELEYLIDGIPYHLTTS
                     FLEPAQDKP"
     gene            4063..>4395
                     /locus_tag="VHS97_00510"
     CDS             4063..>4395
                     /locus_tag="VHS97_00510"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446702.1"
                     /translation="MRNSSAARIVPYAALIASFGLVPPSVRAAEPIVHTLSFPAPETH
                     VAQVQAIVPTGRAASIEIMMAVWSPGFYRVEAYADQVENLEARTTDGAPLMVESTHKN
                     RWRIQTAGE"
BASE COUNT          852 a         1368 c         1328 g          847 t
ORIGIN      
        1 caaggcccac ggataagccc gctcccagaa cgatcggcaa acttttgacg gccacctgct
       61 cgaattgccg gattacttcc ggcaaccgat aaacgatggc cgatggcgcc cgcgcgcacg
      121 ttcgaccgac aaatgcgagg aagccgaacc accgctcgaa ccaaggcgat tcacgggcgc
      181 tcatgccggg tctcccctgc gacgcggggc tcgtcaccaa gattaacgat agtcattcta
      241 tcggtcctca tcccctcgtt ttcatcgtaa ttcgttcgcg acgcacagga tagcgatcgg
      301 aaccgtgcgc gcgtccgtac tacccctgag cgcggccact gccgcacttc tttaaaaagc
      361 cgatcatgcc aaaaatcagg cgttcgtttt tgcctaggat gacaccggtt ggtgtttctc
      421 gtagggttga gtaatctttc gtgagcgaga gggagacgaa gtctaatccg acgacaagga
      481 cgatcggtgg cctggagggg ggggaatcgc gaccgaagcc tcggccgcgc acgctcgtga
      541 gtaatgcatc aatggagcgg caggacggtt tggaggtcgg cgccagcccg tgggaaatct
      601 tcgccttggg tgcgctggcc cctcttgcac cgtacgcaca agttcatctc gcccccaaga
      661 ttccgcccgg cttgctcaat actgcactca tgtcgtactt gtcgcttcaa agcgacgagc
      721 agctgctggc tgtgatcgat gggagcgggg ggagactcgc cggcagttgt gcgctcacga
      781 cgcgccgtat ctattgggcc gcggtcgacg acagcgacga aagccagcca acgaccaaag
      841 gtcgccttcg ggttcgagtc caccggcagt tgctgtgccg ccacgtgctc gagtacgaca
      901 cgttaccgcc ggcggtgatt gaatctcggg gcccgaacgg gtcgttccgg ctcgattttg
      961 gaggaggacg ggccctgaat ttaaagggtg tcgatgcgaa tctggccctg tcgttgcgtc
     1021 agtatttgga caagattcga gccgttgcac gcaacgagtc tgctcctgag cttgccgaaa
     1081 tggagccgga gctcgcagcc cgggtcgcgc gtgcctggcc tgcggtcgcg caggtcagca
     1141 gccgcgctcg aagtctcggt gtcgatttgc gccagttcag gcgatctctc gagacagcga
     1201 ctccccacgc atttgtgacc cccctcctga tcgtggtctg cgtcatcgtc tatggcgtga
     1261 tggtggtttc gggcgtgtcg cctttgtggc ccacgggggc gcaattgatt ctctggggtg
     1321 ccaacgaggg ggctcggatt atcctgggcc atgaatactg gcggctcatc acaagcgtgt
     1381 tcgtgcacgg cggtgtgatt catttggcgc tcaacatgtg gagcctgctg gcgatcggcc
     1441 ccttggtcga gcggctctat ggcaacgcgg cctttgccgt aatttacttg gccgccggta
     1501 tcgggggtgc aatcgccagt gttgcagcgt ctcccgtgcg agtcagcgtc ggggcgtcag
     1561 gcgcgatttg cggggtgctg ggggcgctcc tggcgtttct gctggtgcac cgtcgcgcga
     1621 tcccgacgct cgtcttgaaa ccgcttcgcg ccaatgcgct ggggtacgtc gtattcattg
     1681 caatcctggg cgcggtaatc cccaacatcg atcaggaagc ccatctgggt gggctggcag
     1741 cgggttttgt ggttggattc ttgctctccc gaccctggcc ggccgtcaag agtaagtggg
     1801 ttgccgtccg ccgtgccgtt gtcaccgtct tggtcgccgc cgcgctggcg ggcgcagccg
     1861 ccgccattgc gggcaagggc gcaatgacgc tccctcctca attgcggctt caacacctcg
     1921 agcaacagat tgcgccgccg ctcgaccgtt tcaacgcaat tgccaaagcc acccccggtt
     1981 cactggtcct ccgccgcgat ctcttggtcc cggacgacca tcaaactcac gtgcgcacca
     2041 ttcacgagct gaccgaaggt gcaaatgcaa atttgacgat tcttcgccgc gtgaaaacac
     2101 ccgacccggc ccttcgtaaa ttggtctcct cgctgatcga ggcgcaatcc agccagatcg
     2161 ccatgctcgc ggccgcgcgc cgctttctgg aaaccagaaa cgccgacgaa cttaccggag
     2221 aggctggctt tctcgctgaa aaagccgcaa ctgaccgcag cgtgcgcgcc tttcaagaac
     2281 agcaagtcca ctacctgact gaacgcggct tgatctctaa atccgtgacg aaagagcgct
     2341 aaatgccctt gcacgatttt gggcgggcga ggctctttgg gagggcgagc ctcccggcgt
     2401 gccacggcgc aatccggctc ggacggactt ccgcgagctc agtcgagccg agcctcgccc
     2461 tcccggaatc acgcaagtcc gtttagataa gccgccgcga caacggaagg cgggttgcga
     2521 tgggcgagtg tgtcgtgata tcgtcaccag aagatttgtt gtgcggcgct ccgcccaaat
     2581 caagtgcacc gtatgccgat tttcagtcga accaagtcgg tcaccaaggg agatccgatc
     2641 gtgatggcag agcacaggcg catgtcgaag ttttgtctcc ccattgtcat gctcacggct
     2701 gggctgacgc ttgccgcgag cccgcgcgcg gcgaacgccg ggctcgacga gtatgtcaaa
     2761 aggccggacg gcgcgttcac gtggtccaag atcaacaaac tcagtacccc cgccggaacg
     2821 atcaccacgc tgggtctcac gtcgcaggtc tggcaggaga tcacctggaa gcatgagctg
     2881 acgatttacg agcctcgcga gctctcgtac accgatgcca tgctcttgtt catcaccggc
     2941 ggcaaccaca acagcaagca gaacgataac gatcacaagc agggcttcgg cctggcgcaa
     3001 ctttgcgggg cacggatcgc tgtcttgcgc caggtgccca accaacccct cttggacggc
     3061 aagaccgaag acgagctgat cgctgaaacg ttcgtccgct acctccagac aaaggacgaa
     3121 aactggccct tgctcttccc catggtcaag agtgcggttc gagccatgga tgccctgcaa
     3181 gcctttgcca cggaaaccgg caagccggta actcgatttg tcgtgaccgg gggctcgaag
     3241 cgcggctgga cgacctggct taccggcgcc gtcgatgacc gggtcgtggc gatcgcaccc
     3301 atggtgatcg tgacgctcaa catgggcaaa caaggcccca accagctcaa ggtttggggc
     3361 aagtacagtg aacagatcgg cgactacgtc gagcgcggac tgatggaaaa ggccgacacg
     3421 cccgatggaa ccaagctctg gaagatggtt gatccattca catatcgcga tcgacttacg
     3481 aagcccaagc tgcttattaa cggcaccaac gaccgctact ggacgctcga cgccctggac
     3541 ctctactgga acgggctcga gggaccaaag tacttgatcg agcttcccaa cgccggccac
     3601 ggcctcgatg ccaatcgcga ttgggcattg ggaggcatag gcgcgttctt ccggcacgtt
     3661 gtcaccgacc ggcccatgcc caagatttcc tgggacatgg ctcgcggcgc ggggggtgag
     3721 gcgactttga cgatccacgc cgacccggcc ccgcagtccg cgcgaatctg gaccgcgcgg
     3781 tcgcacagcc gcgacttccg cgagtcacac tgggaatcgg cccccctgaa gccgggcgag
     3841 accatcacgc ggtacgtccc tgccgaaaag agcggcaatc tcgccttctt cggcgagctt
     3901 gaatacctga tcgatggcat accttaccac ctgacaacgt cgttcctgga accggcgcag
     3961 gacaaacctt gaccgcgcgc acttgatcgg aatcggtcgc gtgatcgaac cgttcggcgc
     4021 cgatatccgc ggcgcgcctg acgcgatacg gaggactcga ccgtgagaaa ttcaagtgcg
     4081 gcacggatcg ttccgtacgc ggcattgatc gcgtcattcg ggctcgtgcc tccgtctgtt
     4141 cgggctgctg agccgatcgt ccacacgctc agtttcccgg caccggagac ccacgtagcc
     4201 caagtccaag ccatcgttcc caccggcagg gctgcatcga tcgagatcat gatggcagtc
     4261 tggtctcctg gcttctaccg cgttgaagcg tatgccgacc aggttgaaaa cctggaggcg
     4321 cggacgactg acggcgcgcc actcatggtc gaatcaacgc acaaaaaccg ctggcgtatc
     4381 cagacggcgg gcgag
//
LOCUS       DATAQL010000029         5196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4583025, whole genome shotgun sequence.
ACCESSION   DATAQL010000029 DATAQL010000000
VERSION     DATAQL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4583025"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VHS97_00515"
     CDS             <1..1145
                     /locus_tag="VHS97_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3446703.1"
                     /translation="LAAANASLEKANASKQNLVSRARLALDQAQLSLDEIEERRERNL
                     LARKAASQEDYDKAIAQRKKSAAQVEADQATLEQAEADFKIGIASAAADVERAAAAVE
                     DAKLNLSYCRMFSPIDGRIGELQYKLGNLVGDGSANVLVTIEQLDPMGLDLRPASRYL
                     PIATALTDQGLAVNISVDAPEGEHYYPYTGKTIFIDNKVDENTSTFLARAEVPNPEGA
                     ILPGQYIKATLTVGEYFDAVLVPEQAVLEGQEGTRVYIVDAENKVQLAKVQAVDNYKG
                     LRVLESGVEPNQKVIVEGIQLVRPGQVVKPVEVPLENYETDAAASLYSDPRYTSKVSR
                     VPGMSRRPDPTEPAAKPAGPEPKKAEPEPKKTEPGPAAGAGEPVPK"
     gene            1158..4391
                     /locus_tag="VHS97_00520"
     CDS             1158..4391
                     /locus_tag="VHS97_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3446704.1"
                     /translation="MVNFFIGRPIFATVLALLMMLVGGICIFILPIAQYPEITPPQVQ
                     VTTTYTGADAVTVAETVTTPIEQQINGVKGMIYFSSDSTSNGISTIIATFDVGYSQDI
                     GAVDIQNRVETAQAVLPPEVKQFGVTIKKTSTDMVCVVNLVSPDGRFDSTYLDNYAQI
                     YVLDTLKRVAGVSDVSVFGRKYAMRIWLDPDRMAAQLIAPAEVINAIQSENRQAAAGK
                     IGAQPLPPGQAFKEYPILTKGRLTSVREFEEIVVRRRDDGSIVRLGDLGRVQLDSENY
                     DTAGWLDGKPAGTLPIYQYSDANGLDIVRNVRANMDHLAKSFPPGLEYRINYDTTKYV
                     RENIDEVEHTLVEAFLLVLLVVFVFLQGVRATIIPMLAIPVALVATFALMAAFGFSIN
                     TLTLCGLILAIGLVVDDAIIVVENVEKFLERGDHPLAAVRAAMAEITAPIVTITLVLA
                     AVFVPVAFIPGLTGRLYNQFAMTIVFSFVFSAINSLTFSPAMSRLFLRPKHGESKFFF
                     FRWFNHGMRWLENSYDSFLDFTAHHWWTIVIPSIALLALTYWMLVQRPKAFIPTEDQG
                     YLIASIQTPDGTGKEATARIAKTVGQIAQDLDPGKPGETAGNERPVSSVLVLEGFNVL
                     NSTNQTNSATAFVILKEWSERNKPELRAMALATLLQQRIAAQVRGAVVLVLQPPPIRG
                     LSQTGGFEFMIEDREGRGVQALAKVTDGFLNEARRTEQDRPVYPELGAMFTPFSARVP
                     QLRFALDRVKAERLDVAVSEVFAVLQTNLGGLYVNDFNLYGKVWKVIVQAEGAYRTKP
                     DDIMNLYVLNRKKNKVPLSALGDVKYDLGAIDVPHYNMYNAAKITGQPAPGVSSGQAI
                     AAMERVADAVLPEGFGYEWTGTTFQEQKTGNLAVYIFALSIVCVFLFMSALYESWIRP
                     IYILLTVPLAMFGAVVGLWLYDMPLDVFGQIGLVMLIGLETKNAILVVEFAVELRKKH
                     GASIIDAAKEASRQRLRPILMTSFAFVMGVLPMARATGAGAYSRNSLGIVIAFGMAIS
                     TVLGRFIIPIYLVLGERIIEAFGGARDIMPTPGVEPAAEKNHGVEEP"
     gene            complement(4568..>5196)
                     /locus_tag="VHS97_00525"
     CDS             complement(4568..>5196)
                     /locus_tag="VHS97_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX3446705.1"
                     /translation="VFFMDGGKGHQIVQKDHLEKIQKLTAKGVGLVCLHYAVEVPKGT
                     PGDKFIDWIGGYYETGFSTNPHWTADITKFPEHPTTRGVKPFSIRDEWYFNIRFRPEM
                     KGVTPILAAKPDDKTRQGVSASPRGPYQHIVDAKGREEVLVWTVERPDGGRGFGFTGA
                     HAHKNWGDPNFRKLVLNAILWSAKLEVPADGMECKISEDDLKLNLDAK"
BASE COUNT         1029 a         1525 c         1607 g         1035 t
ORIGIN      
        1 agctcgctgc ggccaacgcg tcactcgaga aggccaatgc ttccaaacag aacttagttt
       61 ccagagcgcg actggccctc gaccaggcgc aattgagtct cgatgagatc gaggagcggc
      121 gcgagcgaaa cttgctcgcg cggaaggcgg cctcgcagga agactacgac aaggccatcg
      181 cccagcgcaa gaaaagcgcc gcccaggtcg aggccgatca ggccaccctc gagcaggcgg
      241 aggccgattt caagatcggg atcgccagtg ccgcggccga cgtcgaaaga gccgcggccg
      301 cggtcgagga tgccaagctg aatctcagct actgcagaat gttttctcca atcgatgggc
      361 ggatcggcga gttgcagtac aagctgggta atctggtcgg cgacggcagt gccaacgtgc
      421 tagtcacgat cgagcagctc gatccgatgg ggctcgacct gcgtcccgcg tcccggtatt
      481 tgccaatcgc cacggccttg accgaccaag gactggctgt caatatatcc gtcgatgctc
      541 ctgagggtga acactattac ccctatacag gcaagacgat attcatcgac aataaggtcg
      601 atgaaaatac gtccacgttc ctggcgcgcg ccgaagtgcc taaccctgaa ggcgcgattt
      661 tgcccggcca gtacatcaaa gccacgctga ccgtgggcga atatttcgat gcggttctcg
      721 tccctgaaca ggcggtcctc gagggtcagg aagggacgcg ggtttatatc gtggacgccg
      781 agaacaaagt gcaattagcc aaggttcagg ccgttgacaa ctacaagggc ctgagagtgc
      841 tcgagtcggg agtcgagccc aaccagaagg tgatcgtcga gggcatccag ctcgtccggc
      901 cgggtcaggt tgtcaagccg gtcgaggtcc cgctcgagaa ttacgagacg gacgccgcgg
      961 cgtctttgta ttcagatccg cgctatacta gcaaggtgtc gcgagttccc gggatgagtc
     1021 ggcggcccga tccgacggaa ccggccgcca agccggctgg acctgagccc aagaaggccg
     1081 agccggaacc caagaagacc gagcctggtc cggctgcagg cgcgggcgaa ccggtgccga
     1141 aatagggatt acgcccgatg gtcaatttct tcatcggacg gccgatcttc gcgacggtgc
     1201 tggcgctgct catgatgttg gtcggcggca tttgcatctt cattttgccg atcgcgcagt
     1261 atcccgagat cacgccgccc caggttcagg tgaccacgac ctatacgggt gccgacgccg
     1321 tcaccgtcgc cgagaccgtt acgacgccaa tcgagcagca gatcaacggt gtgaagggga
     1381 tgatctattt cagctccgac agcacctcca acggcatttc gacgatcatt gcgacatttg
     1441 acgtcggcta ctcgcaggat atcggcgcgg tcgatatcca gaaccgagtc gagactgccc
     1501 aggcggtctt gccgccagag gtaaagcagt tcggcgttac gatcaagaag acgtcgaccg
     1561 acatggtctg cgtggtcaac ctggtctcgc ctgacggccg cttcgactcg acgtacctcg
     1621 acaattacgc ccagatttac gttctggata cactcaagcg ggttgccggt gttagcgacg
     1681 tttccgtgtt cggacggaag tacgcgatgc ggatctggct cgaccccgac cgcatggctg
     1741 cccagttgat cgcgcctgcc gaggtgatca acgcgatcca gtccgaaaac cggcaggcgg
     1801 ccgcgggcaa gatcggcgcc cagccgctgc cgccgggtca ggcgttcaag gaatatccga
     1861 tcctcaccaa ggggcgattg acgagcgtaa gggaattcga ggagatcgtt gtccgccgcc
     1921 gcgacgacgg ctcgatcgtc cggctggggg acctgggccg agttcagctc gactcggaaa
     1981 actacgatac cgctggctgg ttagacggca agccggccgg cacgttgccg atctaccaat
     2041 attccgacgc caatggtctc gacatcgtcc gcaacgtccg ggcgaacatg gatcaccttg
     2101 cgaagagctt cccgcccggg ctcgagtacc gcatcaacta cgacaccacc aagtacgtcc
     2161 gggagaatat cgacgaagtc gagcacacgc tggtggaggc gttcttgctc gtcctgctcg
     2221 tggttttcgt attcctgcag ggggttcgcg cgacgatcat cccgatgctg gccatcccag
     2281 tcgccctggt ggcgacattc gctctgatgg ccgcgtttgg attctcaatc aatacattga
     2341 cgctgtgcgg cttgatcctg gcaatcggcc tcgttgtcga cgacgcgatc atcgtcgtgg
     2401 agaacgtgga aaagtttctc gagcgcggcg atcacccgct agcggcagtg cgcgcagcga
     2461 tggccgagat cacagcgccc atcgtcacga tcacgctggt gctggcggcg gtattcgtgc
     2521 cggtggcatt tatccccggc ttgacggggc ggctctacaa ccagttcgcg atgacgatcg
     2581 tcttttcgtt tgtattctcc gccatcaact cactgacatt cagcccggcg atgtcgcggt
     2641 tgtttttgcg gcccaaacac ggcgagtcga agttcttttt ctttcgctgg ttcaatcacg
     2701 gcatgcgctg gctggagaat tcgtacgact ctttcctcga cttcacggca caccactggt
     2761 ggacgatcgt gatcccgtcg atcgcgctcc tggcgctgac ttactggatg ctggtccaga
     2821 ggcccaaggc gtttattccg accgaagacc aaggctattt gatcgcatcg atccagacgc
     2881 ccgatggcac gggtaaggaa gcgacggcca gaatcgccaa gacggtcggc cagatcgccc
     2941 aagatcttga tccggggaag ccaggcgaaa ctgctgggaa cgaacggccg gtcagctcgg
     3001 ttctcgtgct cgaagggttc aacgtcttga actcgaccaa ccagaccaac tcggccacgg
     3061 cgttcgtcat cttgaaggag tggtccgagc gcaataagcc cgaattgcgg gcgatggcgc
     3121 tcgcgaccct gctacagcaa cggattgcgg cgcaggttcg tggcgctgtg gtcttggtgt
     3181 tgcagccgcc gccgatccgc gggctctccc agaccggcgg cttcgagttc atgatcgagg
     3241 accgcgaagg ccgtggcgtg caggcacttg cgaaagtcac cgacggattt ctcaacgaag
     3301 ctcgccgcac cgaacaggac aggcccgttt acccagagct gggcgcgatg ttcacgccgt
     3361 tctcggcgcg ggtgccgcag ctccggtttg ccctcgaccg cgtcaaggca gagcggctcg
     3421 acgtcgcggt gagtgaagtc ttcgcggttc tacaaaccaa cctcggcggg ctgtacgtca
     3481 atgatttcaa cctctacggc aaggtgtgga aggtgatcgt tcaagccgag ggagcatacc
     3541 ggacaaagcc cgacgacatc atgaatcttt atgtgctcaa caggaagaaa aacaaggtac
     3601 ctttgagcgc tttaggcgat gtcaagtacg acttgggggc gatcgacgtt ccccattaca
     3661 acatgtataa cgcggccaag atcaccggcc agcctgcgcc gggagttagt tcgggacagg
     3721 caatcgcggc catggaacgc gtggccgatg cggtccttcc cgaaggcttt ggctacgaat
     3781 ggaccgggac cacgttccag gaacaaaaga ccggaaacct ggctgtctac atcttcgctc
     3841 tgtcgatcgt atgcgtgttc ctgttcatgt cggccctata cgagagctgg atccgcccca
     3901 tctacatcct cctcacggta ccgctggcga tgtttggcgc ggtcgtcggc ctctggctct
     3961 acgacatgcc tctggacgtg ttcgggcaga tcggattggt catgctgatc ggtctagaga
     4021 ccaagaacgc cattttggtc gtcgagttcg ccgtcgagct tcggaagaag cacggtgcga
     4081 gcatcattga cgcggcgaag gaagcgtcgc ggcagcgact gcgaccgatc ttgatgacgt
     4141 cgttcgcgtt cgtcatgggg gtcctgccga tggcgcgcgc gaccggtgcc ggggcctaca
     4201 gccgcaactc gctggggatc gtcatcgcgt tcggcatggc gatcagtacg gttctgggcc
     4261 ggtttatcat cccgatctac ctggttctgg gtgagcggat cattgaggcg ttcgggggcg
     4321 ccagagacat catgccaacc ccaggcgttg agccggccgc tgagaaaaat cacggagtgg
     4381 aggagccgta gggtggcgtc gagccgcgag cattcgcggc gaggcccacc tgccgaagcg
     4441 gttgttgtat aggccgaccg tcgcccttgg tgggctcacg atccggcgga tcgttcggca
     4501 ccaccctacc acgcgttact tcgctgcctt cgccctgacg gggagagaga ccgcgatgcg
     4561 ccacgactca cttggcatcc agattcagtt tgaggtcgtc ctcgctgatt ttgcactcca
     4621 tgccgtcggc aggaacttcg agcttggccg accagaggat cgcgtttaac accagcttgc
     4681 ggaaattggg atcaccccag ttcttgtggg cgtgggcgcc agtgaatcca aatccacggc
     4741 cgccgtcggg ccgttcgacg gtccacacga gcacttcttc acggcccttg gcgtcgacga
     4801 tgtgttggta tggaccgcga ggagaggcag aaacgccctg gcgcgttttg tcgtcgggtt
     4861 tggcggcgag aatcggtgtg acacctttca tctcggggcg aaagcgaatg ttgaaatacc
     4921 actcgtcgcg gatcgagaac ggcttgactc cgcgggtggt cgggtgctcg gggaacttgg
     4981 tgatgtcagc agtccagtgg ggattggtgg agaagccagt ttcgtagtac ccgccgatcc
     5041 agtcgatgaa cttgtcgccg ggcgtacctt tggggacctc gacggcatag tgcaggcata
     5101 ccaagccaac ccccttggcc gtcagtttct ggattttttc gaggtggtct ttctgaacga
     5161 tctggtgtcc ttttcctccg tccatgaaga agacaa
//
LOCUS       DATAQL010000030         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_461383, whole genome shotgun sequence.
ACCESSION   DATAQL010000030 DATAQL010000000
VERSION     DATAQL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461383"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VHS97_00530"
     CDS             <1..1212
                     /locus_tag="VHS97_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX3446706.1"
                     /translation="HRPDSRLRARLIHTLGDSGLTVQILLDRLNRPGLDPSQRQAILL
                     AAAEATSAGISPSARSGFVDVADAMFHHDTDPGVHSAAELLLRRFGHETRLEQTGEPR
                     SILLDHEASSRGWEVGPNGHTFAILRGPLEFFMGSRLEEDGRQPSEERHYRRIDRSIA
                     VATKEVTADQFRKYRGTYKPQGGSAQTPGIVANSVHWYNAAGYCNWLSREAKIDQSQW
                     CYPEAIDSSSNPEDMPPSGPILGEALKKTGYRLPTEAEWEYFCRAGTETSGPFGESDT
                     LLTRYAWTWRSLKDQVSPPGQLLPNEFGLFDTLGNVLEWCQDGPKDALKDIYQDPYPT
                     GTVVQPASDSFRDELVLDDNFWRYLRGSAYTFPASKARSAFRDEGPAWQSTPARGFRV
                     VRTIPNNHKTN"
     gene            1366..1953
                     /locus_tag="VHS97_00535"
     CDS             1366..1953
                     /locus_tag="VHS97_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446707.1"
                     /translation="MAGKTNPTPILAYGDAQITALALAGTVALPSPFPASARISMVIS
                     PEPSEDGTSTEQRFSLKLVEVIGDKLVVTRFVRVEHVRAIRFRSGRNTNDDPWTWQLK
                     TSRELYADFRTTEDELKAYSQTFKSAGTKFSFEQNLESGTRGIVYVPETAPVLVRTEV
                     AVVAAQAVDIASSYWAAYGKSCWCSGAEPPIKSDV"
     gene            1959..3368
                     /locus_tag="VHS97_00540"
     CDS             1959..3368
                     /locus_tag="VHS97_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3446708.1"
                     /translation="MTRINGVADAMEVDLIFLSRDLSPPRDDVWRSVSEQSGVQVRIH
                     RVTGPRRNDDSNRWETIARARNKGKRLGSAPWVMFVDDDVVLSPWCVARLVDGLSHRP
                     AFAALAADYNKEMSSGWEHWDYPRHVGMGATLFHREHLDRLTFRWETDKCECRCCCDD
                     LRGAGLAIGYLREAEARHNPLPLNRVTDKVVDRGRAIASPSARLSMPNLTGRILAAFD
                     RNHFRLFRHRFLKSLRGSGNHEHVTAVTYGLYPSEQDLLAQTPGVEVIAANPDGHPAV
                     RRLRDFQSVLNRWHEQTPVAYWDAGDVVFQGRLRPLWDLVGTYPDQLLAVTEPTRFAD
                     NGTVQSWVTTIRDPDARQKAMDLFFKNPVINSGFVASTASVMLRYFREAVHLRHSSSL
                     LGSIDWGDQTVLNLYCHSQPHKWREISSGWNFCLCGRKPGEYRVHRDGRTERLDGSPL
                     YIVHGTAGTLNPWDLAFML"
BASE COUNT          782 a         1109 c         1044 g          699 t
ORIGIN      
        1 catcgccccg actcgcgtct ccgcgcccgg ctgatccata cgctgggaga ctccgggctg
       61 acagtccaga ttctcctcga tcgactgaac cggccgggcc tggacccgtc ccagcggcag
      121 gcgatcttgc tggccgcggc cgaggcaacc tcggcaggaa tcagcccgtc cgcgcgttcg
      181 ggattcgtcg atgttgccga tgccatgttc catcacgaca ccgatcccgg tgttcactcc
      241 gccgccgaac tgctcctgcg gcgctttgga catgaaacca ggcttgaaca aaccggtgag
      301 ccgcggagca ttttactcga tcacgaggcg agttcacgcg gctgggaggt cggtcccaac
      361 gggcacacgt tcgcgatcct tcgcggcccc ttggaattct ttatgggatc ccggctcgag
      421 gaggacggcc gacagccctc ggaggaacgg cactatcgcc ggatcgaccg gtcaatcgcc
      481 gtggcgacca aggaagtcac cgccgatcaa ttccgcaaat atcgagggac ctacaaaccc
      541 caaggcgggt ccgctcaaac gcctggcatc gtcgccaact cggtccattg gtacaacgcg
      601 gccggctact gcaactggct cagccgcgag gccaagattg accagagcca gtggtgctac
      661 ccggaggcga tcgatagcag cagcaatcca gaggacatgc caccctctgg cccgatcctg
      721 ggagaggcct tgaaaaagac aggttaccgg ctcccgacgg aggcggaatg ggagtacttc
      781 tgtcgggcag gcactgaaac gagcggcccg ttcggggaat ccgacacgct actcacccgc
      841 tacgcgtgga cctggcggag cttgaaagac caggtttccc cgcccggtca gctcctgccc
      901 aacgagttcg gactttttga cacgctcgga aacgtcctgg aatggtgcca ggacggaccg
      961 aaggatgcat tgaaagacat ataccaagac ccatacccga ccgggactgt cgtccaaccc
     1021 gcttctgaca gttttcgcga cgagcttgtc ctggatgaca atttctggcg ctatctccgt
     1081 ggctcggcat acacgttccc agcatcgaaa gcgcgctccg cgttccggga cgaaggcccc
     1141 gcgtggcagt ccactcccgc gaggggcttc cgtgtcgtga ggacaattcc gaacaaccat
     1201 aagacgaact aagagatggt caactcaaaa gtcaatcaac gttatcgaaa caaattttgt
     1261 tgacacgatc cggaatggcc tcaacaatcg aatcgatcgg caacgacggt acgcgggcga
     1321 gcgctcgtgt atgtccggat gctgacggtt tcaaggagtg ttgccttggc tggcaaaact
     1381 aacccaacac cgatccttgc gtacggcgat gcgcaaatca ccgcgctcgc cctcgcagga
     1441 accgtcgcgt tgccatcgcc gttcccggca tcggcccgga tctcgatggt aatctcgccg
     1501 gaaccttcgg aagacggcac ctcgactgag caaagattct ctctcaagct cgtcgaagtg
     1561 atcggggaca aactggtcgt aacaagattt gtaagagtgg agcacgtccg ggccatacgc
     1621 ttccgttctg ggcgtaatac gaacgacgac ccgtggacct ggcagctcaa aacctctcga
     1681 gagctttacg cagactttag gacaaccgag gacgaattaa aggcttactc ccaaaccttt
     1741 aagagtgccg gcacaaaatt ctcgttcgag caaaacctcg aatccggaac gcgagggatt
     1801 gtctacgtcc ccgagacagc cccggtcctg gttcgcactg aagtcgcagt cgtagcggca
     1861 caagccgtgg acatcgcttc ttcgtattgg gcagcctatg ggaagtcgtg ctggtgttcc
     1921 ggtgccgagc ccccgatcaa gtccgatgtg taaacgggat gacgaggatt aatggggtcg
     1981 ccgatgccat ggaagtcgat ctgatatttc tgagccgcga tctgtctccg ccccgagacg
     2041 acgtctggcg ctcggtctca gagcaatcgg gagtgcaggt ccggatccac cgcgtgaccg
     2101 gtcctcgccg caatgacgac tccaaccggt gggagacaat cgcccgtgcc cgcaacaagg
     2161 ggaagcggct gggttcggcc ccttgggtca tgttcgttga cgacgatgtc gtcctgagcc
     2221 cttggtgcgt ggcacggctg gttgacgggt tgagccatcg tcccgcgttc gccgcgctag
     2281 cggccgatta caacaaggag atgagctcgg gatgggaaca ctgggattac ccccgacacg
     2341 tcggaatggg cgcgacactc tttcaccggg aacatctcga taggctgaca ttccgctggg
     2401 agactgataa gtgcgaatgt cggtgctgct gcgacgacct ccgcggcgct ggactagcga
     2461 tcggctatct gcgcgaagct gaggcccggc acaatccatt gccgctgaat cgcgtcaccg
     2521 acaaagttgt cgatcggggc cgagccatcg cttcaccttc cgctcggctg agcatgccga
     2581 atttgacggg acgcatcctc gccgcgtttg accgaaacca ctttcgcctc tttcgtcaca
     2641 gatttctcaa gtcattgcga ggatcgggca atcacgaaca cgtgacggcc gtcacatatg
     2701 ggctctaccc aagcgaacaa gacctcttgg cccagacgcc cggagtcgaa gttatcgcgg
     2761 ccaaccccga cggccatccc gcggtacgcc gactacgcga ctttcagagc gtccttaatc
     2821 gctggcacga acaaacgccc gtcgcgtatt gggacgccgg cgatgtcgtt tttcaaggcc
     2881 gactcaggcc gctttgggac ttggtcggca cctaccccga ccaactattg gcggttacgg
     2941 agccgaccag atttgctgac aacggcacgg tccaaagctg ggtaacaacg attcgggatc
     3001 ctgacgcacg gcaaaaggca atggatcttt tcttcaagaa tcctgtcatc aacagtggat
     3061 tcgtcgcaag caccgcgtcg gtgatgctac gttattttcg tgaagccgtt caccttcgcc
     3121 actcgagctc gcttctcggt tcgatcgatt ggggagacca aacggtattg aatctctact
     3181 gtcactctca gccgcacaag tggcgcgaaa tatcctcggg ctggaacttt tgtctttgcg
     3241 gccgcaagcc gggtgaatac cgcgtccatc gcgacgggcg aaccgagcga ctcgacggct
     3301 cgcccctgta tatcgttcac ggcaccgcag gcacccttaa cccatgggac ctagcgttca
     3361 tgctttgagc aaagcgcaag gcaactcgcc gccagccgct tctcggtgag ctcatggaca
     3421 aacggggaca tcgaggacaa aaggggacat aattaagtcg aggacaaaag gggacataat
     3481 taatattgag cgtccgagga tggtacttta caatcgtggc atgccaagaa ctgccagagc
     3541 ctcggttggc ggcatgtggt atcacgtgct caaccgcggc gaggccgtct tccataagcc
     3601 cgccgactac gacgccttcg tcgaggccat catc
//
LOCUS       DATAQL010000031         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4583064, whole genome shotgun sequence.
ACCESSION   DATAQL010000031 DATAQL010000000
VERSION     DATAQL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4583064"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VHS97_00545"
     CDS             <1..241
                     /locus_tag="VHS97_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446709.1"
                     /translation="AFVMAHPGVASAILGPRTMQHLDDLLAGANVVLKDDILDRIDQI
                     VPPGADVGPLPAAYNPPAVTQANLRRRPAIGRAAA"
     gene            574..1446
                     /locus_tag="VHS97_00550"
     CDS             574..1446
                     /locus_tag="VHS97_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3446710.1"
                     /translation="MSPISNLESGIRIPGISSHFLDSARPGDQGQGRLRYLTGGTGKP
                     LLLLHTVRTQAEHFRHLIPLVQNRYTVYALDLPGLGYSQIVSGASYEEPDMRAAVKHL
                     ITRLDLHDVTLLGESMGAVLALTTAADLPERVRGVLAINPYDYAGGIMRSSLLARLII
                     SGVLAPVIGPIIAGVEPKPIMRAVLRGGIVDKTALREDYLDELLRVGRRPGYAFVARS
                     VYASLPSFVAARSRYPDVRAPVHFIYGEEDWSRTLDRKANKELIPVAKFTQVPNAGHF
                     IALERPEVLANLLP"
     gene            1467..>1700
                     /locus_tag="VHS97_00555"
     CDS             1467..>1700
                     /locus_tag="VHS97_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446711.1"
                     /translation="MARRVRNHALRPRLPDATIVKPTDAVIRVSRACICGSDLGHERT
                     RGTTGSLEGNIMAIELQLGTVLQASPLKELGFSA"
BASE COUNT          356 a          543 c          476 g          325 t
ORIGIN      
        1 ggccttcgtg atggcacacc cgggtgtcgc gtcagcgatt ctcggcccgc gcaccatgca
       61 acacctggac gatctgcttg cgggcgccaa tgtcgttctt aaggacgata ttttggatcg
      121 gatcgaccag atcgtgcccc ctggcgcgga cgtcggaccg ctcccggcgg cttacaaccc
      181 gccagcggta acacaggcca atttgcgtcg ccgaccggcc atcggccggg cagccgcctg
      241 accgaaccgg catcctcacg ctgatactgc acagatttgc cccatcaaaa tggtgggccc
      301 gctcaccatc gaattagcgc agaaagagcc tatttttata gatgtttcgc caattcggtt
      361 gagccagtta cccaggaagt tacccatctg aaataccggc gtgtttcagg ttaaagatgg
      421 ccttaactcg ccaggttcag acggcctgaa cgacgcaatg gcgagcattt gacgatgcat
      481 gtatatacat ataatatagc taaccaaaga ctagtcctgg cgcctaaaca agcgggtcga
      541 gaagagacgg ctcaggatgc cgcaggagat gccatgtcac caatcagcaa ccttgaatcc
      601 ggaatcagaa ttccgggcat cagcagccac ttccttgact cggcgcgccc cggcgaccaa
      661 ggccagggcc gactgcgcta cctcacgggc ggaacgggca agccattgct cttgttgcac
      721 accgtacgca cccaggccga acatttccgg cacctcatcc ccttggtcca gaaccggtac
      781 accgtgtacg ctctcgatct gccagggttg ggttactcac agatcgtgtc aggcgcctcc
      841 tacgaggagc ccgacatgcg cgccgccgtc aaacatctca tcacgcggct cgacctgcac
      901 gacgtgacac tgctcggaga gtcaatgggt gccgtgctcg ccctgacaac agcagccgat
      961 cttcccgagc gagttcgagg cgtgctggcg atcaatccct atgactacgc cggcggaatc
     1021 atgaggtcca gcctcctggc tcggctgatc atcagcggcg tgctcgcgcc cgttatcggt
     1081 ccgataattg ccggagttga acccaaacca atcatgcgcg ccgtgctccg tggcggcatc
     1141 gtcgacaaga ctgcgctgcg ggaggactac ctggatgaac tgctcagggt cggtcgacgg
     1201 cctggctacg cgttcgttgc ccgttccgtg tacgccagcc tccccagctt cgtcgcagcc
     1261 cgctctcgtt atcccgacgt cagagcgccg gttcatttca tctatggaga ggaggactgg
     1321 tcgcgaacgt tggaccggaa ggcaaacaag gagttgattc ccgtggccaa attcactcag
     1381 gtgccaaacg cgggccactt catcgctctg gaaagacctg aggtgctagc aaacctcctc
     1441 ccttgaatcc gcgtggccga aaaaaagtgg cgcggcgagt ccgaaaccac gcgctacgcc
     1501 caaggctccc tgacgccacc attgttaaac ccaccgacgc cgtgatccgg gtcagccgcg
     1561 cctgcatctg cggcagcgac ctaggtcacg agcgaactcg tggcacgact ggctcgctcg
     1621 aggggaacat catggcgatc gagctccagt tgggcacagt gttgcaggct tcccctttga
     1681 aggaactcgg ctttagcgcc
//
LOCUS       DATAQL010000032         7697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3667332, whole genome shotgun sequence.
ACCESSION   DATAQL010000032 DATAQL010000000
VERSION     DATAQL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3667332"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VHS97_00560"
     CDS             <1..299
                     /locus_tag="VHS97_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3446712.1"
                     /translation="VGEFKDQNVRLIAVNLQETAEQITAMLERQKLHVTVALDRDGVV
                     AENYKAAAIPQTVIINRDGNVARLFVGGGAHLEDQLKEAIKSVLNGDKPQEPKK"
     gene            complement(331..2001)
                     /locus_tag="VHS97_00565"
     CDS             complement(331..2001)
                     /locus_tag="VHS97_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3446713.1"
                     /translation="MSAVSGLTARFMLGLGLLAIVGWPAIATVREAAMAWSDRPGVRG
                     AAGGSLDPAASAELLRDTGEMARPLRLAIETGGLVAATLALVLPLGVLLALFLFRTDA
                     WGRGPLLALIGLSAFVPLPLQATAWLGALGNAGRAQAFGIRPLLVGHLGAAVVHALAS
                     LPWVVLIVGVGLCAVEPELEESALLDYGPIGVVFRVTLRRAWGAIAAAALAVAVLTAG
                     DMTVTDLLQIRTYAEEAYLQYSLGRGPGGAALVAIPPLVVLGLLILIVGRFLGRLDPA
                     RLVSSFTRARVWQLRRWRIPCGALLAVLIGNTAAVPLYALVWRAGRVGGRATLGLAPH
                     WSLAGLTGTLRNAGEEIWEPLQQSLVCTAVAATVTAFLACALAWASRTSFGWRCVALV
                     TVALTLATPGPVAGMALVLAYRNLPSVYDSPAMVVMAEALRTLPYALLILWPFLRSFP
                     QEHLDAAALDGLKPWGQFVWVVLPLSRRAIVAAWTISFAIGLGELPATNLVAPPGTQP
                     MPVVIWGLLHTGVESHLSGVALIMLIVVGAAGIIAASAVRLLRLPATD"
     gene            complement(1998..2822)
                     /locus_tag="VHS97_00570"
     CDS             complement(1998..2822)
                     /locus_tag="VHS97_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3446714.1"
                     /translation="MKLGLINSAWAQAGRGTAFGIQETKKIGFDSIDIFADPLEIDAK
                     EKRLIKDECDRARLPIVSVACVAVGLVDFNVSVQRFHIERVRAYLDMAYLFEAKNVLL
                     VLGEYIWQKEVIPPAEQWTTAVKHVRTLGEYADHLGLEIALELEPFDLSLLNDVASMA
                     RFLKDVDHPAVKANLDISHLVLADQPAALIEVLRGRVAHVHISDCDGKVHGDLPPGRG
                     VVDFVPYLRAIKALEIPEVAVSIELEYSPEPEKIVEWVSEAYDRTAALLRSAGLRS"
     gene            complement(2919..4457)
                     /locus_tag="VHS97_00575"
     CDS             complement(2919..4457)
                     /locus_tag="VHS97_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3446715.1"
                     /translation="MNVSSYASLVTMLSPVFAWAVDVPSLVLISVGLVALSITAWLQA
                     RQWRLRHSILSSDFQTVAAERSQAELARVQTEVFYHSLVETIPQMILCKDLEGRFTFA
                     NNKFCAELGTTLDNIKGKTDLDFFPRELAEKYRRDDKRVLESGEILDIVEEHVTPRGE
                     KLYVQVMKTPLSGADGEPIGVQGIFWNVTERMRAEEQLREQNVTLQELARSEREAHEA
                     LKTAQSRMVETEKLASLGQLVAGVAHEINNPLAFVSNNVAVLERDLRDLKTLIGLYRQ
                     VERSGDVGHAELNGQIDAVSEQLDVVYTLDNMPRLIERTRDGLRRIERIVKDLRLFAR
                     VDEGEWNEVDLNPGIESSVNMVQGYARKKGVRLATDLEPLPMVRCRGARIHQVVVNLL
                     MNAIDACSADETVTIRTRSEAEGQVVRIDVADTGCGIEPAIRERIFDPFFTTKAIGEG
                     TGLGLSISYGIVQEHHGTIEVQSEPGAGSCFRIRLPREPQRSHSRGPLAAQGVGSAVE
                     APTS"
     gene            complement(4454..5569)
                     /locus_tag="VHS97_00580"
     CDS             complement(4454..5569)
                     /locus_tag="VHS97_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3446716.1"
                     /translation="MSAIETANLNHRPLLPERRDVGIGVVGAGFIVRDCHLVAYAEAG
                     FRVVGITSRSIDKAREVAELRRLPRVYESLEQMLADPAIDVIDMAVPPGDQPGVIDRV
                     LERPRRIRGILAQKPLAMSYGVAKRLVHACKEAGVLLQVNQNMRYDHSVRALKTLLDR
                     RVLGEPVLATIEMRAIPHWMPWAERGRSLSTFLMSIHHLDTFRYWLGEPERVIASTRP
                     DPRTRFSHSDGINLYILEYANGARASGWDDVWAGPAREGAGAEYAVRWRVEGTQGLAL
                     GTIGWPGWPQRVASTIDYSTIADQGAWHRPRWNDAWFPDAFAGTMSGLLRALETGEEP
                     DIAGSDNLKTIALCEAVLAAAHDHRITRLDEFTGNSP"
     gene            5806..6330
                     /locus_tag="VHS97_00585"
     CDS             5806..6330
                     /locus_tag="VHS97_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446717.1"
                     /translation="MLGGPASSIQVMTPAEVARLIDTHSAPLVLYARQWCDAPEDVVQ
                     EAFVKLVRQGRPPEDTAAWLYRVVRNGALDEAKMARRRLRRESVAARPVRWFVEPEVD
                     GLDAQTAVAALETLAPERREVIVAHHWGGLSFEQIAAVAGCSASTAYRRYTAGVEDLR
                     NHLGVICPKNSSNA"
     gene            6763..>7697
                     /locus_tag="VHS97_00590"
     CDS             6763..>7697
                     /locus_tag="VHS97_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446718.1"
                     /translation="MCRTLFSCAALALLGLPGRADDTIVASETLVRLSVTPALAPKPA
                     LRYQLLPELKEMNPGNPIQNYLKCFMEQQKFFFDKDAFERRERLLTVPLKELPAQELT
                     DYGGFALKQADWAARLDNPDWQILLKVKTDGVALLLPDVQQMKPLANALKVRFRAEVA
                     LSRFDDAIRTAKTMFAISRHMSEHPTLVGNLVGISIAYLAIGPLEEMLEHPQCPNLYW
                     ALTNLPVPMVPIDKGMGGERALVLSEFRGLDERSPMTGDELDKFITHTDILIGEGKPP
                     KSGESRVRAWLDARTKDDKTVIAARHRLIECGFPQ"
BASE COUNT         1497 a         2461 c         2377 g         1362 t
ORIGIN      
        1 cggtcggcga attcaaagac caaaacgtgc gattgatcgc ggtcaacctc caggaaacgg
       61 ccgagcagat aaccgccatg ctcgagcgcc aaaagctgca cgtgactgtc gccctcgacc
      121 gcgatggcgt ggtggccgaa aattataaag ccgccgcaat accccagaca gttatcatca
      181 accgcgacgg caatgtcgcg cggcttttcg tgggtggcgg cgcgcacctg gaagaccagc
      241 tcaaagaggc gatcaagtcg gtgctgaacg gcgacaaacc gcaagaaccc aagaaatagg
      301 aggggcgttt cctgcaacgc tttttgtctg tcagtccgtt gcaggcaggc gcagcaatct
      361 caccgccgag gccgcgatta tccctgcggc gccgactacg atcagcatga taagggccac
      421 gcctgacaag tggctctcga caccggtatg aagcagaccc cagatcacca ccggcatggg
      481 ttgagttccc gggggagcaa ccaggttggt ggcgggcagc tcgcccagtc caatggcaaa
      541 ggaaatcgtc cacgctgcca cgatcgcccg gcgggataac ggcaacacga cccacacgaa
      601 ttgcccccag ggtttgagtc catcgagtgc agccgcatcg agatgctctt gaggaaaaga
      661 acgcaggaac ggccagagaa tcaagagcgc gtacggcaga gtccgtagcg cctcggccat
      721 gactaccatc gccggtgagt cgtacaccga gggaaggttt cgataagcca gaacgagcgc
      781 catgccggcg acagggccag gggtggcgag cgtgagcgcg accgtgacca aggccacaca
      841 ccgccagcca aatgacgtgc ggctggccca cgcgagcgca caggcgagga aggccgtaac
      901 ggtagcggcc acagccgtgc acacgaggct ctgctggagc ggttcccaga tctcttcacc
      961 tgcgtttcgc aaggtgcccg tgagtcccgc gagcgaccag tgtggggcga ggccgagcgt
     1021 ggctcggccc cctactctcc ccgctcgcca taccagggcg taaagcggca ctgcggccgt
     1081 gttgccgatc agcacggcaa gtaacgcgcc gcacggaatc cgccagcggc gcagttgcca
     1141 gaccctggcc cgagtaaatg atgagaccaa tcgcgcgggg tcgagtcgac cgaggaaacg
     1201 accgacgatc aagatcagca atcccaggac gacgagcggg ggtattgcga ccagggccgc
     1261 cccccccggc ccgcggccca gactgtattg caagtacgct tcctcggcgt atgtgcgtat
     1321 ctgcaagaga tcggtgactg tcatatcgcc ggcggtaagc acggcgactg ccagcgccgc
     1381 cgcggcgatc gcgccccatg ctcgccgcaa cgtcactctg aacacgaccc cgattggacc
     1441 gtaatcgagc aacgccgact cttccagctc gggctccacg gcacagagcc ctacccccac
     1501 gatcaggacg acccaaggca ggctggccag cgcgtgcacc accgcggctc cgaggtgtcc
     1561 gaccaataat ggccgaatac cgaacgcctg ggcgcggccg gcgtttccga gtgcgccgag
     1621 ccaggcggtc gcttgcaagg ggagcggcac gaaggccgac aggccgatca aggccagcag
     1681 cggaccgcgg ccccaggcgt ctgtgcgaaa gaggaagagc gccagcaaaa cacccaaagg
     1741 cagcacgagc gcaagagtcg cggcgaccaa gccgccagtc tcgatcgcga gtcggagcgg
     1801 ccgtgccatc tctcctgtgt cgcgcagcag ctcagcggag gcggccggat cgagcgaacc
     1861 gccggccgct ccccgcacgc caggtcgatc cgaccaggcc attgccgcct cccggacagt
     1921 cgcgatcgcc ggccagccga caatcgccaa gagccccagg ccaagcatga atcgggccgt
     1981 cagtcccgaa accgcgctca cgaacgtaat cccgcactgc gcaagagagc cgccgtacgg
     2041 tcgtaagcct cactgaccca ttccacaatt ttctccggct ccggcgaata ctccagctcg
     2101 atcgagactg cgacttcggg gatttcgagg gccttgatcg ctcggagata gggtacgaaa
     2161 tcgaccaccc ccctccccgg cggcaagtcg ccgtgcacct tgccgtcgca gtcggagata
     2221 tgaacatgcg cgacgcgccc tcgcagcact tcgatcagcg cggccggttg atcggccagt
     2281 accaagtgcg agatgtcaag attggccttt acggcagggt gatcgacgtc cttaagaaac
     2341 cgggccatgc tggcgacatc gttcaagagc gaaaggtcga acggctcaag ctcaagcgcg
     2401 atctccagac ccagatgatc ggcatattcg cccagggttc gcacgtgctt gaccgcggtc
     2461 gtccattgct ccgcaggggg gattacttct ttctgccaga tgtactcacc cagcaccagc
     2521 agcacgtttt tggcctcgaa caggtaggcc atgtcgagat aagcccggac tcgctcgatg
     2581 tgaaaccgct ggacgctgac gttgaaatcg accagtccga ccgccacgca ggcgacgctg
     2641 acgatcggca atcttgctcg gtcgcactcg tccttgatca ggcgtttctc cttcgcgtcg
     2701 atctcgagtg gatcggcgaa gatgtcgatc gagtcaaagc cgatcttctt ggtttcttgg
     2761 attccaaacg ctgttccgcg acctgcctgg gcccaggcgg aattgatcag tcccagtttc
     2821 attgttggtc ccacgcacta tagattgatg caacggcgcc aggcgccctg ataatcaacg
     2881 tgtcacggcg ttccggcccg tgtcaagcgg cgccacggtc aagaagtcgg tgcctccacg
     2941 gccgatccca ctccctgagc ggccagtggg ccgcggctat gagatcgctg cggctcgcgg
     3001 gggagccgga tacgaaagca cgagccggcg cccggctcgg attggacctc gatcgttccg
     3061 tggtgctcct gcacaatccc gtagctgatc gagagcccca gtccagtgcc ttcgccgatg
     3121 gccttggtgg taaagaacgg gtcgaagatc cgttcgcgaa tcgccggttc gatgccacaa
     3181 ccggtgtcgg cgacgtcgat tcgtaccacc tgcccctccg cttcggaccg cgtgcgaatc
     3241 gtgaccgtct cgtccgcgga gcaggcgtcg attgcgttca tgagcaggtt gacgacgacc
     3301 tggtgaatcc tcgccccgcg gcagcgaacc atcggcaagg gctcgaggtc ggtggcgaga
     3361 cgaacaccct tcttgcgggc gtagccctgg accatattca ccgacgactc gatcccgggg
     3421 ttcaggtcaa cctcgttcca ttctccctca tcgaccctgg caaagagccg caggtccttc
     3481 acgattcgtt caatccgacg caggccgtcg cgggtgcgct cgatcaagcg gggcatgttg
     3541 tccagggtat aaaccacgtc gagctgttca ctgaccgcgt cgatctggcc gttcagttcg
     3601 gcgtgaccga cgtcgcctga gcgttcaacc tggcggtaaa ggccgatcaa agtcttcaag
     3661 tcgcggagat cgcgttcgag cacggcgacg ttgttgctga cgaatgccag gggattgttg
     3721 atctcgtgag ccacgccggc cacgagctgg cccaagctcg cgagcttttc ggtctcaacc
     3781 atgcgactct gtgcggtctt gagcgcctcg tgcgcttcgc gttccgagcg ggccagctct
     3841 tgcagagtca cattctgctc ccggagctgt tcctcggcgc gcatacgctc ggtgacgttc
     3901 cagaaaatcc cctggacgcc gatgggctcg ccgtcggccc cggaaagcgg tgtcttcatt
     3961 acttgtacat agagcttctc gccccgggga gtgacgtgct cctcgacgat gtcgaggatc
     4021 tcacccgact ccagcactcg cttgtcatca cggcggtact tttccgccag ctctctgggg
     4081 aagaagtcga ggtcggtctt gcccttgata ttatcgaggg tcgttccgag ctccgcgcag
     4141 aatttgttgt tggcgaaagt gaagcgcccc tcgaggtcct tgcacaagat catctgggga
     4201 attgtttcga ccagagagtg atagaagacc tcggtctgca cgcgcgccag ctcggcctga
     4261 ctccgttcgg cggcgacggt ctggaaatcg gatgacaaga ttgagtggcg caatctccac
     4321 tggcgggcct ggagccacgc cgtgattgat agcgcgacca gtccgacgct gatcaagacc
     4381 agcgagggga cgtcgacggc ccacgcgaag acagggctga gcatggtcac taatgaggcg
     4441 tacgatgaaa cattcacggg ctgtttccgg tgaattcgtc gagccgcgtg attcggtggt
     4501 cgtgtgcggc ggccaaaacg gcttcacaaa gtgcgatcgt cttgaggttg tccgaccccg
     4561 cgatgtccgg ttcctcgccg gtttcgagcg cgcgcagtaa tccgctcatt gtgccagcga
     4621 atgcgtcggg aaaccacgca tcattccagc gcggtcgatg ccaggcgccc tgatcggcga
     4681 tcgtcgaata gtcgatcgtg ctggcgacac gctgtggcca acccggccag ccgattgttc
     4741 caagcgccag cccctgtgtc ccctcgacgc gccagcggac cgcgtactcg gccccggccc
     4801 cttcacgcgc gggcccggcc cagacgtcgt cccaccccga ggcgcgggcg ccgttggcgt
     4861 attcgagaat gtagaggttg attccatcgc tatgcgaaaa cctggttcgc gggtccggcc
     4921 gagtgctcgc gatgacccgc tccggctcgc ccagccagta acggaacgtg tcgaggtggt
     4981 gaatgctcat gaggaacgtc gagagcgacc ggcctctttc ggcccagggc atccagtggg
     5041 ggatcgcccg catctcgatc gtcgccagca ccggttcgcc cagcacccgc cggtcgagga
     5101 gggttttcaa ggcccgcacc gagtggtcgt agcgcatatt ctggttgact tgaagtaaca
     5161 caccggcctc tttgcacgcg tgcaccagac gcttggccac gccgtaagac atggccagcg
     5221 gcttttgagc aagaatgccc cgtatgcgtc gcgggcgctc gaggacgcgg tcaatgacac
     5281 cgggctggtc gccagggggg acggccatgt cgatgacgtc gatggcggga tcggccagca
     5341 tctgttcgag cgactcataa acgcgcggca gacgcctcag ttccgccact tcgcgcgcct
     5401 tgtcgatcga tcgcgacgtg attcccacta cccgaaagcc ggcctcggcg taagccacaa
     5461 gatggcagtc gcggacgata aaccccgcgc ccaccacgcc gatgcccacg tcccgccgct
     5521 cgggcaggag tggacgatga ttcaggttgg cggtctcgat cgcactcatt ggggactgga
     5581 ccagcatcga ggcctgcaac ttatgcccgc gtgggatatt cgaattaacc gcacgctatt
     5641 gacgctagat tctgacgatc tctactccca aacctattcc cacccaacgc ccgtgtccag
     5701 ggggcgcgga attccggctc acggacgtgg ccggcgaaag tggccgggac ctcattgcct
     5761 gagcgagcta tttgacgaca tcaagcgatt aagaattttg gcggaatgct cggggggcct
     5821 gcgtcgtcta ttcaggtaat gactccggcg gaagttgcac ggctgatcga cacgcattca
     5881 gctccgctgg tgctgtacgc acggcaatgg tgcgacgcgc ccgaggacgt tgtccaggag
     5941 gcttttgtca agctggtccg ccagggccgg ccgccggagg acaccgccgc ctggctctac
     6001 cgcgtggttc gtaacggtgc cctcgatgaa gccaagatgg cccgaaggag gctgcgacgt
     6061 gagtcggtgg ctgccaggcc ggtccgctgg ttcgtcgagc cggaagtgga cggcctcgac
     6121 gcccaaaccg ccgtggccgc tctcgagaca ctggcgcccg agcggcgcga ggtcattgtg
     6181 gcgcatcact ggggaggatt gagttttgaa cagatcgccg cggtggccgg ctgttcggcc
     6241 agcactgcct accggcgcta caccgccggg gtcgaagatc tgcgcaatca tctgggggtg
     6301 atatgtccga agaattcctc gaacgcctga gccggttcaa accggatgcc ggcgggttgg
     6361 accgagacgc cttgctcttc gccgccggcc gcgcctctgc ccgcccgaac cgtggctggg
     6421 ttgcgctggc cgcgctactc gcaaacacgc aaatgctgtc actcgtcctt ctgtggccgc
     6481 gcccgatgcc cccggcgggc agatcgacta ttgcggcgga cgtcgcccca gttctctcga
     6541 catcggcaac tctcgagcct ccgacgtccg aggcctgggc aaacgctggc ctgtggtcgg
     6601 cccgaaatag tcggcgggct ttggaagccg aagatcgccc gaccggcgac gtgaccttca
     6661 tcgatagcgg gccaccgttg cgggcgtcgg gcccagttcc gccgtagctc ctgaactgag
     6721 ccctcgcgac ctcatttctc gccgtttgcc aggagtcgtc ccgtgtgccg aacccttttc
     6781 tcttgcgcag cgcttgctct acttggtctc cctggccggg ccgacgacac gattgtcgca
     6841 tcggaaacac tggtccggct gagcgtgacg ccggcgcttg cccccaaacc ggcactccgc
     6901 tatcagctcc tgcctgagct caaggagatg aatcccggca acccaatcca gaactacttg
     6961 aagtgtttca tggagcaaca gaaattcttc ttcgacaagg acgcattcga acgccgcgaa
     7021 agactgctga ccgtgccgct caaggaattg cccgcgcaag aactgacgga ttacggcggt
     7081 ttcgccctca agcaggccga ctgggccgcg cggctcgaca acccggattg gcagatcctg
     7141 ctcaaagtga agacagatgg agtcgccctg ctgctacccg acgtgcagca gatgaagccg
     7201 cttgccaacg cgctcaaggt gcggttccgg gccgaggtcg ccttgagccg ctttgacgac
     7261 gccatccgaa ccgccaagac gatgtttgcg atatcgcggc acatgagcga acacccaaca
     7321 ctcgtgggca atctggtcgg catttccatc gcgtacttgg cgatcggccc cctggaggag
     7381 atgctggaac acccgcaatg ccccaacctt tactgggccc tgacgaacct gcccgttccc
     7441 atggtcccga tagataaagg gatgggagga gagcgggcgt tggtgttgtc ggaatttcgc
     7501 ggtctggacg aaaggtcccc tatgactggg gacgaactcg ataagttcat cacgcacaca
     7561 gacatactga tcggagaggg caagccaccc aaatcgggcg agagtcgggt gcgggcgtgg
     7621 ttggacgcgc ggaccaagga tgacaagacg gtaattgctg cacgtcaccg tcttatcgag
     7681 tgtggcttcc cccagga
//
LOCUS       DATAQL010000033         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3667429, whole genome shotgun sequence.
ACCESSION   DATAQL010000033 DATAQL010000000
VERSION     DATAQL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3667429"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2106)
                     /locus_tag="VHS97_00595"
     CDS             complement(<1..2106)
                     /locus_tag="VHS97_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX3446719.1"
                     /translation="MLTTLLTYAVLLLGQTTGGALAPWASQPHATDVAKQHVQAITDR
                     RVEYVVIQGGTMDGRNCRSPQGVWQPFEQIWESNRSVRMENAGATDVVNPWLSNGHND
                     FRSLDKIVARAIEPQMTDSEKARALWWQEVQHRFHWEGDNGELLDPVKVFNVYGHNTC
                     GNDSICLAGLWRKAGLKVAPARLVGHCVTQVFYDGSWHLMDGDMQSIYLLRDNETIAG
                     EQDLVRDHDLIRRTHTQGMLQTDGRAGDEWESSIYVFEGQVAGDRNSAASALNMTLRP
                     GEAIVWRWGHLNPIKYHGPRPPRFPDTVCNGLWEYRPDFKQPSWRAGATTALSVRERD
                     GSLMAEKGKTGIVVWAISSPYVVVGGRLEVDGTGATFKLSWDGVSWREIDGNLDGFFP
                     SEGPARYRYYLKCELSGDAQLRRLGITNDLQMAPLTLPGMGVGTNAFTYTDESASGRR
                     MRITQQWVERSASRPPVAPPEPVFPPAAGVTDGTGISFQWRPATDPDGDAISDYHFEL
                     SARADMKWPLSMSFAKLISRTADAGQARYTLPGPGLLNPGTKYFWHVRARDDKGVWGP
                     WSPTWSFTPRGPAPPRDVYLEFDRERGRGVLRWAPNPVGRKPMAYRVYASDEKGFSVS
                     DRPYTVTVGESKEMPSVFPANFVVETSAAELAVVGPHVKLEGANKAFYRVVAVDAAGN
                     RSGPSDYAASPRPVIVTAPV"
BASE COUNT          445 a          810 c          788 g          473 t
ORIGIN      
        1 aaccggggcc gtgacgatga ccggccgcgg ggaagcggcg tagtcggaag gcccgcttcg
       61 attccccgcc gcgtccaccg caactactcg gtagaaggcc ttgttcgccc cctcgagttt
      121 cacgtgcggg cctacaaccg ccagttcggc ggccgaggtt tcgaccacga agttcgcggg
      181 gaacaccgag ggcatttcct tggattctcc caccgtgacc gtgtagggcc ggtcgctgac
      241 ggaaaagccc ttctcgtcgc tggcatagac acggtacgcc atgggcttgc ggccgactgg
      301 gttcggcgcc cagcggagga cgcctcgccc gcgctcgcgg tcgaattcga ggtagacatc
      361 tcgaggaggt gccggccccc tgggcgtgaa gctccaggtt ggactccaag gtccccagac
      421 tcccttgtca tctcgcgctc ggacgtgcca gaaatacttc gtgcccggat tcagaaggcc
      481 ggggcccggc aacgtatagc gcgcctggcc ggcgtcggct gtccgagaga tgagcttggc
      541 gaagctcatc gagaggggcc acttcatgtc ggcgcgtgcg gagagctcga agtggtagtc
      601 gctgatcgca tcgccgtcgg gatcggtagc cggccgccac tggaaactga tccctgtccc
      661 gtcggtcact cctgccgcgg gaggaaagac cggttcgggc ggcgcgacgg gcgggcggct
      721 cgcggaccgc tcgacccact gctgggtaat tcgcatgcgg cgtccgctcg ccgactcgtc
      781 ggtgtaggtg aacgcgttcg tgccgacacc cataccggga agagtgaggg gggccatctg
      841 caggtcgttc gtgatcccga gccgccgcag ctgtgcgtct cccgacagct cgcatttcag
      901 gtagtatcgg taccgagcgg gcccctctga agggaagaac ccgtcgagat tgccatcgat
      961 ctcgcgccag gacacgccat cccaagagag cttgaacgtc gcgccggtac catcgacttc
     1021 gagccttcca ccgacaacga cgtatgggct cgaaatcgcc cagacgacga tgcctgtttt
     1081 tcctttctcc gccatgagac tgccgtccct ttcccggacc gacagggcgg tggtggcgcc
     1141 cgcgcgccag gacggctgtt tgaagtcagg tcggtattcc cacaaaccgt tgcaaaccgt
     1201 gtcggggaac ctggggggcc ggggcccgtg atacttgatc gggttcaaat gcccccagcg
     1261 ccacacgatc gcctcgcctg gacgcagcgt catgttcaag gcgctggcgg cagagttgcg
     1321 gtcccccgcg acctgtccct cgaacacata aatggaagac tcccactcat cccccgcgcg
     1381 cccgtcggtc tggagcattc cctgagtgtg agtacggcgg atcagatcgt gatcacgcac
     1441 caggtcctgc tcaccggcga tggtctcgtt gtcgcgcagg aggtaaatgg actgcatgtc
     1501 gccgtccatc aagtgccatg agccatcgta gaagacctgt gtgacgcaat gccccaccag
     1561 acgggccggc gcgactttga ggccggcctt gcgccacagg ccagccaggc atatggaatc
     1621 gttgccgcac gtgttgtggc catacacatt aaagactttt acaggatcaa gcagttcgcc
     1681 attgtcgcct tcccaatgga agcgatgctg cacttcctgc caccagaggg ccctggcctt
     1741 ctcgctatcg gtcatctgcg gttcgatggc cctggccacg atcttgtcga ggctgcggaa
     1801 gtcgttatgg ccattggaga gccacggatt gacgacatcg gtcgcacccg cgttctccat
     1861 ccggacggag cgatttgact cccagatctg ttcgaagggt tgccagaccc cctggggcga
     1921 ccggcagttc cggccgtcca tcgtgcctcc ctggatcacg acgtactcga ctcgtcgatc
     1981 agtgatcgct tgcacgtgct gctttgccac gtcggtcgcg tgcggctgcg acgcccatgg
     2041 ggccagcgcg ccgccggtcg tctgcccgag aagcaggacg gcataggtca acagtgttgt
     2101 cagcatgcgc atctctccct cacgaccacg atcggctcgc ccacacgacg gatgaggtga
     2161 acgagacgcc cattgtatcc ttggagtccg cgcttcgtct gtcccgattc acggcgttcg
     2221 cttgatggct gtttctccgt cgcttaggct tacagaggca aggtcaggag tcagcaggag
     2281 gcggccccat gatcgccggc cagacgcctg cgatgaactc ttcgcgagta gaatacgccg
     2341 aacgtcgaca gcatcaaggt agaaaccgct catgacatac tgcaaggccg tcgttgtaat
     2401 cgtggtcgcg tccctttccg cgcagcttgc catcggccag gagtggccga aggagccgat
     2461 cccggcaccg aggatccgcg acgagatcaa ggcccacgag tctggcctgg cagtgt
//
LOCUS       DATAQL010000034         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3820332, whole genome shotgun sequence.
ACCESSION   DATAQL010000034 DATAQL010000000
VERSION     DATAQL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3820332"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..>2452
                     /locus_tag="VHS97_00600"
     CDS             495..>2452
                     /locus_tag="VHS97_00600"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3446720.1"
                     /translation="MSTSARGFARHDDRFDLRILASGVFPNYNPENYKKLREGFAMRQ
                     LFVFGWTFVVASAAMAETVKVASIEGVTEYKLANGARVLLFPEASRPTVTVNMTVLVG
                     SRHEGYGESGMAHLLEHMVFKGTPYHPEVPKALRDHGASFNGTTNSDRTNYFETMPAT
                     DQNLEFGIALESDRLVNSFVKGEDLLSEMTVVRNEFERGENNPGSILNQRIYSAAYEW
                     HNYGKSTIGSQSDIERVPIDNLQAFYKKYYQPDNVVLVVAGKFEEAKALALVEKYLGS
                     IPKPTRKLDATYTEEPAQDGERTVTLRRVGAVGSAGVAYHIPAASHADWAPLSLLGGI
                     ISQSPNGRLYKALVESKLATSASGRADNSHDPGLFFASASCPPEKLDAVRDALVKTFE
                     SLGEVPFTEDEVNKAKVRNKRSAELLQLNSQAMAQALSSASSHGDWRLLFIQRDRVAA
                     VTASDVNRVAKAYFQKPNRTVGLYIPAKESTRLAVPSAPTIDFVVKDYKGGSVGAAGE
                     AFDPSPANLDSRIKIIKESGLKAGLLHKQNRGETVSLVLTLHYGTEDSLKDQVTAAGM
                     LPGLMMAGTKKHDRQALREELDSLGIRIGTGGGGGGRGGRGGRGGGGAGGTAGQLTFS
                     VDAKRDTLPQALKLLGEILREPAFPADE"
BASE COUNT          539 a          759 c          714 g          440 t
ORIGIN      
        1 cgacaacagg tctcgagcat cgctgccgca tcctccagcc actgcgcttt atccagatcg
       61 gcagcaagga ggaagcacgt ttcatctcgg agcagcggat aagcgcccat gacgaagtcg
      121 cgcccggagt cgtcgtgtcc agaaagatgc cagcgcacaa cttggtccgt gacgggcaag
      181 aagtgttgat tcagacacgc ggcacttgat cctgggcttc tcgcacaagc cggttaccca
      241 ttcattgcca caagcaggtg aatatccgga cttgccagtc ttgcgattct cgaagcggcg
      301 aggataaaca tcgtctcgcc cgcgaaagag cgagcgaaac agagcgatct tggactcagg
      361 cggggacagg tgatggacaa taccgtcgga ctggctcatg tgaaacgcct gccggacacg
      421 actcgcctca tcgaaaaaca tcgaatcact cgctcgacgt aagaaccata ctacaccttg
      481 cgtggaggaa gtcgttgtct acgtccgcgc gcggcttcgc cagacacgac gatcgctttg
      541 acctccgcat actagcaagc ggcgttttcc ctaactataa tcccgaaaac tacaaaaaac
      601 ttcgggaggg attcgccatg cgtcagttgt ttgtgtttgg ttggactttc gtggtcgcgt
      661 cagcggcgat ggcggagacg gtcaaagtcg cctcgatcga gggtgtgacg gagtacaagc
      721 tagccaacgg agcacgggta ctactctttc ccgaggcgtc gcggccgacg gtgaccgtga
      781 acatgaccgt gcttgttggc agccgccacg aaggctacgg cgaatccggc atggctcacc
      841 tgctcgagca tatggtcttc aagggcacgc cgtaccaccc ggaagtgccg aaggcgcttc
      901 gcgatcacgg ggccagcttt aacggcacca cgaattccga ccgtaccaat tacttcgaga
      961 cgatgcccgc caccgaccaa aacctcgagt ttggcatcgc tctcgagtcc gatcgtctcg
     1021 tgaacagttt cgtcaaaggc gaggatctgc tgtcggagat gacggtcgtc cgcaacgagt
     1081 ttgaacgtgg tgagaacaac cccggctcca tcctcaacca gcgaatctac agcgcggcgt
     1141 acgaatggca taactacggc aaatccacca tcggcagcca atccgacatt gagcgcgtgc
     1201 ccatcgacaa tctacaggcc ttctacaaaa agtactatca gccggataac gtcgtcttgg
     1261 tcgtggccgg caagttcgag gaggcaaagg ccctcgccct cgtggaaaaa tatctcggtt
     1321 cgatcccgaa accaacgcgc aagctcgacg ccacctacac cgaggagcca gcgcaagatg
     1381 gcgaacgtac ggtcacgctg cgccgcgttg gcgcggtggg ctcggcgggc gttgcctatc
     1441 acatcccggc tgcctcgcac gccgactggg caccgctgag tctcttgggc ggcatcatct
     1501 cgcagtcccc caacggccgg ctttacaagg ccctggtcga atcaaagctg gccaccagcg
     1561 cgtccggccg tgcggataac tcgcacgatc ctgggctctt tttcgcttcg gcgtcatgcc
     1621 cgccggaaaa gctcgacgcg gtgcgcgacg ccctggtgaa aaccttcgaa tctctcggtg
     1681 aagtgccatt cacagaagac gaagtcaaca aggccaaggt ccgcaacaag cggagcgccg
     1741 agttgctcca attgaacagc caggcgatgg cccaggcttt gagttcggca tccagccacg
     1801 gcgactggcg actgcttttc atccagcgcg atcgcgtcgc cgccgtgacg gcgtccgacg
     1861 tcaatcgagt ggcgaaagcc tacttccaga agcccaaccg cacggtcggc ctctacatcc
     1921 cagccaagga atcgacgcgt ctcgcagtgc ccagcgctcc caccatcgat tttgttgtca
     1981 aggattacaa aggcggctca gtcggagcag cgggcgaagc cttcgacccc tcgcccgcga
     2041 atctggattc gaggatcaaa atcatcaaag aaagtggcct gaaagccggc ctgttacaca
     2101 agcagaatcg gggcgagacg gtctcgctcg tgctgacctt gcactacggc accgaagact
     2161 cactcaagga tcaggtaacc gcggccggaa tgctccccgg cctgatgatg gccggcacta
     2221 agaaacacga tcgccaggcg ctccgcgaag agctcgattc actgggtatc cgcataggaa
     2281 cgggtggcgg cgggggcggt cgcggtggtc gcggaggtcg cggtggcggc ggagccggcg
     2341 gtaccgccgg gcaactgacc ttctctgttg atgccaaacg agacaccctc ccgcaggcgc
     2401 tcaagctgct cggcgagatc ctgcgcgagc cggcctttcc cgcggacgag tt
//
LOCUS       DATAQL010000035         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5346915, whole genome shotgun sequence.
ACCESSION   DATAQL010000035 DATAQL010000000
VERSION     DATAQL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5346915"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..652)
                     /locus_tag="VHS97_00605"
     CDS             complement(23..652)
                     /locus_tag="VHS97_00605"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX3446721.1"
                     /translation="MLISPIDRDALRAQVRQSKPVPNFKIDNFLDEEFANRVLAAFPT
                     FEEALKVGRTFQAVNEYKKVQLTDASLFAEPVAELNQALAAPEFLEMLSYVFDIPDLL
                     ADDQLVGGGIHQTGPRGHLDVHIDFNYIEDRQLHRRLNILIYFNKDWRPEWGGNIELW
                     NKDVTVCHHSFSPIFNRCVVFITNEISFHGVTAVKCPADRTRKSFAAYY"
     gene            complement(732..>2281)
                     /locus_tag="VHS97_00610"
     CDS             complement(732..>2281)
                     /locus_tag="VHS97_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEX3446722.1"
                     /translation="PRVVATLIPTKKNQPARDFLMGFGAAYGREVDGVWRFELPADVA
                     ATVVYSPTATAADDPIGDSDPVPVRTPSEPAGQPRKSERFQRIAVELSRPEHVLEAAR
                     ARPAGQGVRPELGQPLVAARNEIESELARIWADLLRLDEVGIRDSFFDLGGTSLRAVD
                     LFAQIDNRLGKTLPLTSLIEAPTIEQLARLVAGGADRDSLVLIRDGRDLPPLFLVHDG
                     DGETMLYRNLALLLSDEHAVYGLQPHSLPNVPMAHSRIDDMAAYHIGKMRSIQPRGPY
                     LVGGMCAGGVIAFEIALQLQKQGENVALVAVIDAADVAATRKAWRTAGQRIRRFSSVM
                     GQDQSIRFDQRIVAVLKTVAAKARNLTAYLIGQRLKEWRDDIRMRQFRYRLDRGRALP
                     QSLRDIPVRTAYMFAEKNYRPDGLFGGRLVLFRATCGEANDEPYIERYLDPLLGWGRR
                     AKEDILVHDVPGGHSSMLQEPNVRVLAVQLQTAIDDVLADVESPSREPARKDSGMLSA
                     RPVPTIR"
BASE COUNT          443 a          683 c          716 g          439 t
ORIGIN      
        1 agccaaccgg tgcctctttg gtctagtagt aggcagcgaa agattttcgc gtacgatcgg
       61 ctgggcattt cacggccgtg accccatgaa agctgatctc gttggtgatg aagaccacac
      121 accggttgaa gatcggcgaa aaggaatgat ggcacaccgt gacgtctttg ttccacagct
      181 cgatgttgcc cccccattcg gggcgccagt ctttgttgaa gtagatcagg atgttgagcc
      241 gtcggtggag ctggcgatcc tcgatgtagt tgaagtcgat gtggacgtcc aagtgaccgc
      301 gcgggccggt ctgatggatt ccgccgccga caagttggtc gtcggccaag aggtcgggaa
      361 tgtcgaacac gtaactgagc atttcgagaa attccggcgc cgccagcgct tgattgagct
      421 ctgcgaccgg ctcggcaaaa agagaggcgt cggtgagctg cactttcttg tattcattca
      481 cggcctgaaa ggttcgaccc accttgagcg cttcctcgaa ggtcggaaac gcggcgagga
      541 cacggttggc gaattcttca tcgagaaagt tatcaatctt gaaattgggg accggtttgg
      601 attgacgaac ttgcgcccgg agcgcatcac ggtcgatggg gctgatgagc atggctttcc
      661 tcaaggctga gtcattcaat cgaggttgac acagagttcc gggcgatcca ggatttctag
      721 gtgccgggcg gctaacggat cgtcgggacc ggtcgggcag acagcattcc tgaatccttc
      781 ctcgccggct cacgcgaagg gctctcaacg tccgcgagga cgtcatcgat tgccgtctgc
      841 aattgtaccg ccaagacgcg gacgttgggc tcctggagca tgctcgagtg accgccgggg
      901 acatcgtgaa ccaaaatgtc ttcttttgca cgccgacccc agccgagcag aggatctagg
      961 tagcgctcga tgtagggttc gtcgttcgcc tcaccgcagg tcgccctgaa caacacgagt
     1021 ctgccgccga agaggccatc cggccgataa ttcttctcgg cgaacatata ggccgtccgc
     1081 acggggatgt ctcttagaga ctgcggcaaa gcccgaccgc gatcgaggcg gtaacggaac
     1141 tgcctcatcc ggatgtcgtc gcgccactcc ttgaggcgtt ggccgatcag ataggcggtc
     1201 aggttcctgg ctttggccgc gaccgttttg aggaccgcca cgatgcgctg gtcgaatcgg
     1261 atcgattggt cctggcccat cacggaggaa aagcgtcgaa ttcgctgacc tgccgtgcgc
     1321 caggccttac gggtcgcagc cacgtcggcc gcgtcgatca ccgcaaccag cgcaacgttt
     1381 tccccttgct tctgtaactg gagggcgatc tcgaaggcga tcacgccccc ggcgcacatc
     1441 cccccgacca gatacggacc ccgcggctgg atcgagcgca tcttgccgat gtggtaggcg
     1501 gccatgtcgt cgattcggct gtgggccatg ggcacattgg gcaaggaatg aggctgaagt
     1561 ccgtagaccg cgtgctcgtc actaagaagt aaagccaggt tgcggtatag catcgtctcg
     1621 ccgtcgccgt cgtgaaccag aaagagcggc ggcaagtctc tcccatcgcg gatcaaaacc
     1681 aacgaatccc gatcggcgcc acccgcaacg aggcgggcga gctgctcgat cgttggagcc
     1741 tcgatcaacg aggtcagggg aagcgtcttg ccgagccgat tgtcgatctg cgcgaagagg
     1801 tctacggccc gtagcgaggt tccccccagg tcgaaaaaac tgtcacggat cccgacctcg
     1861 tcgaggcgaa gtaagtcggc ccagatacgc gcgagctctg actcaatctc gttgcgcgcc
     1921 gcgaccaaag gctggcccag ctcgggacgg acgccctggc cagccgggcg ggcccgcgca
     1981 gcctccaaaa cgtgctccgg ccgggagagc tcgacggcga tccgctggaa tcgctccgac
     2041 ttgcggggtt gcccggccgg ctcgctgggc gtacgcacgg gtactgggtc gctgtccccg
     2101 atcgggtcgt cagccgctgt ggccgtgggg ctgtaaacga ctgtcgcggc cacgtcagcc
     2161 gggagctcga agcgccatac accgtcgacc tcccgaccgt acgccgcccc aaatcccatc
     2221 agaaagtccc gggccggctg attcttcttg gtcggtatca gggtcgcgac gacccttggc
     2281 a
//
LOCUS       DATAQL010000036         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_919629, whole genome shotgun sequence.
ACCESSION   DATAQL010000036 DATAQL010000000
VERSION     DATAQL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919629"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VHS97_00615"
     CDS             complement(<1..732)
                     /locus_tag="VHS97_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3446723.1"
                     /translation="MAAEFGDRTAFSVVDIGPITFAEWDGAANALARRLLADGLEPGG
                     RVGIHLQPDHALRWLVAYTAAHRAGGVAVPMNPRLAPAEVAHVLAHSAAQVVVADGDL
                     VVRDLESGAGRLAFVIDAGAAGSVVPSPPDAFSPVLAWDDAVTGDRSPFQAPREKDDL
                     ADILYTSGTTGRPKGVAVRHSNGSMVGAVEPNWSGGGWVHSSPLFTFAGISLVYTPMK
                     FGLQVLYQPRFDADRWLRLVEEEHPV"
     gene            986..>1637
                     /locus_tag="VHS97_00620"
     CDS             986..>1637
                     /locus_tag="VHS97_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446724.1"
                     /translation="MKRVRVRFVFASVFCVLVPAVLAIQPEVGASNTSTQVTTSLQSW
                     YQKTGHSVLQAFVADARRFAHVTASTTPREASQDCNRFTTDVLAAEHKKLPPNSVLAS
                     DYRYYLQTAATRFKECVTGIAEKSEFELAEGAQGGSLAAQAAMTIIRGTQSGKVVNLP
                     PSSTNLLPSLSASVVVPKCYADFKSLEVAIEAYNAQNNAFPAPPAAWNAATYSDNFA"
BASE COUNT          331 a          505 c          538 g          263 t
ORIGIN      
        1 cacgggatgc tcctcttcga cgagacgcag ccaacggtcc gcatcgaaac ggggctggta
       61 gagcacctgc aggccgaact tcatcggcgt gtacaccagc gaaatgcctg cgaaggtgaa
      121 gagcggactc gaatggaccc atccgccgcc cgaccagttc ggctcgacgg cgccgaccat
      181 cgacccgttc gagtggcgca cagccacacc cttgggacgc ccggtcgtcc cagacgtgta
      241 gaggatgtcg gccagatcgt ctttctcgcg gggcgcctga aagggagagc ggtcccctgt
      301 gacggcgtcg tcccaggcga ggacgggcga gaaagcgtcc ggcggagacg gcacgacgga
      361 gcccgccgcg ccggcgtcga tcacgaaggc gaggcggcca gcaccggatt cgaggtcgcg
      421 cactacgagg tcgccgtcgg cgacgaccac ctgggcggcg gagtgggcca ggacgtgcgc
      481 cacctcggcg ggggcgagac gcgggttcat ggggaccgcg acaccgcccg cccggtgggc
      541 ggccgtgtag gcaacgagcc agcgcagcgc gtggtcgggc tgcaggtgga tgcccacgcg
      601 accaccgggc tcgaggccgt cggcgaggag gcggcgggcc aacgcattgg ccgcaccatc
      661 ccactcggcg aacgtgatgg ggccgatgtc gaccacgctg aacgccgtgc ggtcaccgaa
      721 ctcggcggcc atgcggccca gatgctcgtc caataacacg attgaggatg gtagccatga
      781 agaagccggg ccgtcggact gtccggattg gttcgaccgt ttgggtcaag gggtccggtt
      841 gcgctctttc ccatgatcca agagaaactt gctcatattg acggagggat tagtgcctta
      901 tgctgcatgc agacacgcgc caatcggcca aaggggcaca cgtgcccaaa cgcattggca
      961 gaatggcaga atgaggggga aggcgatgaa gagagtccgt gttcgctttg tgttcgcgtc
     1021 ggtcttctgc gtgttggtgc ccgctgtcct ggccatccag ccagaggtag gcgcctccaa
     1081 tacgagcacg caagtcacca cgtcgttgca aagttggtat cagaagactg gtcactctgt
     1141 cctccaagcg ttcgtggcgg atgcccggcg attcgcacac gtgacggcat ccacgacacc
     1201 gcgtgaagcg agccaggatt gcaaccgctt cacgaccgat gtactcgcgg cagaacacaa
     1261 gaagcttccg cccaattcgg tcttggcctc ggactaccgc tactaccttc agacggccgc
     1321 gacgaggttc aaggaatgtg tgacgggcat tgccgaaaaa agcgagttcg agctggccga
     1381 gggagcacag ggtggttcgt tggcggctca agcggcgatg acgatcatca gagggaccca
     1441 gagcgggaag gtcgtgaacc tcccaccctc gagcaccaat cttctgccat cactatccgc
     1501 atcagtggtc gttccgaagt gctacgcgga cttcaagtca cttgaagttg cgatcgaggc
     1561 atacaacgca caaaacaacg ccttccctgc tcctccggcc gcgtggaatg cggcaaccta
     1621 cagcgacaac ttcgctc
//
LOCUS       DATAQL010000037         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4430998, whole genome shotgun sequence.
ACCESSION   DATAQL010000037 DATAQL010000000
VERSION     DATAQL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4430998"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VHS97_00625"
     CDS             <1..526
                     /locus_tag="VHS97_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEX3446725.1"
                     /translation="THGRSIADEVKRLLRGRWTELPAPPQITLERFDLPFDTLPTRTQ
                     LEQLVQAGGPPGYNASVLLAKLNRGEPLPHDLPYSTQTWKFGDKLLMVFLPGEVVVDY
                     VLRLKKEFDRDRLWVTAYANDVPCYIPSERILREGGYEGGGAMVYSGRPTRLKRGVEQ
                     LIIDAVHRCAGAGF"
     gene            complement(608..1939)
                     /locus_tag="VHS97_00630"
     CDS             complement(608..1939)
                     /locus_tag="VHS97_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3446726.1"
                     /translation="MKTASHDLSVPVSSAGAMPRVSTVPLLMMVVATAHFNRISISVA
                     GAERIVAEYGVSPERMGLIYSSFLLFYTLAMLPGGWFIDRFGARTALLSLGFGSAIFV
                     ALTGVVGLLANGAQSVCLGLIVVRSLLGLTNAPLHPGSARMVHESAPPESRAFANGWV
                     TFSACLGIAASYYVMGTLIDHFDWPIAFLISGSFTLIVALVWTVRTRGWRRPLNAFAG
                     GTSVRADLSALFRVLRRRSVVCVTLSYTAYGYFQYLFFYWIEYYFETIQGQGRGVSRG
                     YSMMITLAMGAGMVSGGWLADRVPRSFSRRVRMLLVPTIGMIASGVVFEIGVFAPHPQ
                     ATLVAFVIAAALLGMCEAGFWTTVVELGGTYGGTAAGLMNTGGNAGGTLSPYLTPLFS
                     GYFAARHGTDLGWRLSLAVAGVVLIAGAVVWWGINPADDSSNQPTLEPVTA"
     gene            complement(1966..2994)
                     /gene="hisG"
                     /locus_tag="VHS97_00635"
     CDS             complement(1966..2994)
                     /gene="hisG"
                     /locus_tag="VHS97_00635"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX3446727.1"
                     /translation="MSTGLIDPIRLALPSKGHLYDGIVELLKTAGYKVWRASDRQYEA
                     TIVDHGRFHVVFMRPTDIVIQVQEGRCHLGVTGKDVFAEHAVETDVARVVVPNLGYGG
                     CRLAVAVPESWIDVSHVMDLVELTTEFKAAGRTFRVSTKYPALVRQHFRKFGIYHYQL
                     IDSEGALELHPSLGIADIIVDLTSSGTTLKDNRLREVEEGTVLESAACLIGHAPSLAT
                     LAAEGESGPLALLLDAIDGVRGAEGWLHLEVVGSQAAPGPETAAQVAAHLREQGASHV
                     ARGEIWDEAGKPGWRVTALLSARKLAGCRRALFRMGASRVVGLPPRIVFDRDGSSTFD
                     ELRLQLSN"
BASE COUNT          680 a         1091 c          908 g          540 t
ORIGIN      
        1 cacgcacggc cgttcgatcg ccgacgaggt caaacgcttg cttcgcggtc gatggaccga
       61 gctgccggcc ccgccccaga tcacactcga gcggttcgat ctcccgttcg acacgttgcc
      121 cacacgcacg caactcgagc agcttgtcca ggccggcggc ccgcccggat acaacgcgtc
      181 ggtcctgctt gccaagctca atcgcggcga gccgctcccg cacgacttgc cgtactcgac
      241 gcaaacatgg aagtttggcg acaagctgct catggtgttc ctccccggcg aagtcgtcgt
      301 cgactacgtt ttgcggctca agaaggaatt cgatcgggac cggctctggg tcaccgcata
      361 cgccaacgac gtcccttgct acattccttc cgaacgcatc ctccgcgagg gtggatacga
      421 aggaggcgga gccatggtct attccggacg gcctacacgg cttaagcggg gcgtcgaaca
      481 gcttatcatc gacgccgtcc accgatgcgc cggcgcggga ttttagacca gcgttactgt
      541 gtaccacgac ggatccctgg tttgtgtact tccactactc ggctcatttg cgacttcgcc
      601 acaatcatca tgccgtcacc ggctcaagag ttggttggtt actcgagtcg tccgccggat
      661 tgattcccca ccacaccacg gcccccgcga tcaggacgac tccggcgacc gccaggctca
      721 accgccatcc caagtccgtg ccgtgtcgcg ctgcgaagta accgctgaag agtggcgtga
      781 ggtacggcga gagcgtgccg ccggcgttac cgcccgtgtt catcagcccc gcggccgttc
      841 cgccgtaggt accgcccagt tccaccaccg tcgtccagaa cccggcctcg cacatcccga
      901 gcagcgcggc cgctatcaca aaagccacca atgtggcttg tgggtgagga gcaaacaccc
      961 cgatctcgaa gaccacgccg ctggcaatca tcccaatcgt gggcacgagc aacatgcgca
     1021 cgcgccgact gaacgaacgt ggcacgcgat ccgccagcca tccgccactg accatgccgg
     1081 cccccatcgc cagtgtgatc atcatcgaat acccccgcga cacgccgcgc ccctggccct
     1141 ggatcgtctc gaaatagtat tcgatccagt agaagaacaa gtactggaaa taaccatagg
     1201 cggtgtagct gagcgtgacg cagacgacgc tgcgacggcg aagaactcgg aacaaggccg
     1261 agagatcggc gcgcaccgaa gttccacctg caaaggcatt caacggtcgc cgccaaccgc
     1321 gcgtgcggac cgtccacacc aaggccacga tcaaagtgaa actgcccgag atcaggaagg
     1381 cgatcggcca gtcaaagtgg tcgatcaagg tccccatgac atagtagcta gcggcgattc
     1441 ccaaacaggc ggagaatgtc acccacccat ttgcaaacgc ccgcgactcg ggcggcgcac
     1501 tctcgtggac catccgggcc gagcccggat gcaacggggc attggtcagg ccgagtagcg
     1561 atcgcactac gatcagtccc agacacaccg actgtgcacc atttgccaac agccccacaa
     1621 ctccagtgag tgcgacaaat atggccgagc caaatcccag cgacagaagc gcagtccgcg
     1681 ctccaaaacg atcgataaac cagccgccag gcaacatcgc caacgtgtag aacaagagaa
     1741 acgacgaata aatcaaaccc atccgttccg gcgaaacacc gtattcggcg acgatccgct
     1801 ccgcgccggc cacggaaatg ctgatgcgat tgaaatgcgc cgtcgccacg accatcatca
     1861 aaagcggaac cgtgctgacg cgcggcatcg cgccagccga cgacacgggc acagagagat
     1921 cgtggctggc agttttcata tatacatgtc gtttcgctta ctctcttaat tgctcaactg
     1981 aagtcgcagt tcgtcaaagg tagaggaccc gtctcgatcg aagacgatac ggggaggtag
     2041 cccgacgacg cggctggcgc ccatgcggaa gagggcgcgg cggcagccgg ccagcttgcg
     2101 ggcagacagc aaggccgtca cgcgccagcc aggcttgccc gcctcgtccc agatttcgcc
     2161 acgtgccacg tgcgacgcac cttgctccct gaggtgagcg gcaacctgcg cggccgtctc
     2221 ggggccaggg gcagcctggc tgcccaccac ttccagatgg agccagccct ccgcgccgcg
     2281 cactccgtcg atcgcgtcga gcaagagagc caagggacca ctctctccct ccgccgccag
     2341 cgtggccagg ctgggcgcat gcccgatcaa acacgcggcc gactcgagca ccgtcccctc
     2401 ctcgacctcg cggagccgat tgtccttgag cgtggtcccc gaactggtca ggtcgacgat
     2461 gatgtcggca atgcccaggc tggggtgcag ctcgagagct ccttccgaat cgatcaactg
     2521 gtaatgatag atgccaaact tgcgaaagtg ctggcgcacg agggccgggt acttggtcga
     2581 cacgcggaaa gtccgcccgg ccgccttgaa ctcggtcgtc agctcgacca agtccatgac
     2641 gtggctgacg tcgatccagc tctcgggaac cgcgacggcc agccggcagc cgccatagcc
     2701 gaggttggga accaccacgc gcgccacgtc ggtttccacg gcatgctcag caaacacgtc
     2761 cttgcccgtc acacctagat ggcagcgccc ttcctgcacc tggatcacga tgtcggtcgg
     2821 ccgcatgaac acaacgtgga agcgaccgtg atccacgatc gtcgcttcat attgccggtc
     2881 gctggcacgc cagaccttgt agccggccgt ctttaacagc tccacgatcc cgtcgtacag
     2941 gtgccccttc gacggcagcg ccagccggat ggggtcgata agtccggtac tcatccgcca
     3001 agcttccttc gtttcgtggt ccgcgtgtgt ggattgttga tattgttcgg aaacccaatc
     3061 acttcgctag ggccaatacc gctaaaataa cagtaagttt ctggcgcatc acaaatgaaa
     3121 atcccgcccc ccttgtgggg gagggttagg gtggggggcg tcactctcga ggaccaaagc
     3181 cacgccgatt tctgacactt gcctcccccc accccaacc
//
LOCUS       DATAQL010000038         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466 k141_4032,
            whole genome shotgun sequence.
ACCESSION   DATAQL010000038 DATAQL010000000
VERSION     DATAQL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4032"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VHS97_00640"
     CDS             <1..1049
                     /locus_tag="VHS97_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3446728.1"
                     /translation="LIAQSNGAKFIALSAVLAGVREVRAAIDEAKKSQRRDARTILFI
                     DEIHRFNKAQQDALLGAVEDGTVSLIGATTENPSFEVNSALLSRCRVVILEPLGPDDV
                     ATIIRRALVDSERGLAGRRPHLTPDLIDKLASWSAGDARVALSTLEDAVLATAPAEDG
                     TRPVAESTLVEALGRARYAYDRQGEDHYNLASALIKSVRNSDASAALYWLARMIEGGA
                     DPIFIARRLCILASEDIGLADPQAIVQAAAAAQITHLIGLPEALFPLAQATIYLAQAP
                     KSNKVMTAYTAAATDAAASAREPVPLHLRNAVTSLMKGIGYGGGYRYIHDDPAAQTER
                     RCLPERFEDRNYLE"
     gene            complement(1096..3288)
                     /locus_tag="VHS97_00645"
     CDS             complement(1096..3288)
                     /locus_tag="VHS97_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3446729.1"
                     /translation="MSTRMKARGASLAILVCVVGSGRAHADVPEDKEIAATIARGVAF
                     LKDAQSPQGHWDEHAQNEHRLGMTALAGLALMENGVARDAPEISRAREIVSEMATTSD
                     QTYDIALAILFLARCQQGRRGEADALIRTLGQRLAGGDREGIWDYQVPRSELEPVPAK
                     TRSRGAAGKRGARRPQFFPSQGDNSNTQFALLGLWAASRHGYDSDQALEAIDHHFRSS
                     QLRDGRWGYRIGMNGKDSMSCAGLMGLTIAASRPSLAERQTARARGAMLAADPAFQSA
                     LKAVGQDARRAGLHSDIYYLWSLERVCVALGLRTLDGFDWYAHGARILIELQQDDGGW
                     PHGQWGRLPATCLALLFLRKANLAFEIDRVLRLPGSGNDLHVVKTSGPGSAESDGQAP
                     GAADSTPAANVSPAAETTGEAGAGDVRVNVTAASEQAFPRISVQFEVKRPDGSFLLDA
                     GRDDFGVTEEGREVDVVEFRAPLTTEAIPTTVVLVVDHSLSMEEEDRIGGLKRAVRSF
                     LEKLPEGSRIALIAFGSEVDRLASFTTDRGQIKTAVDALEPAGATRFYDAVAAALELL
                     DHETGRRAVLALTDGEDTFSQSANLESVIAGAARIGLPVYTLGLGSEEEIESADLRRL
                     AVSTRGQYYPARHADQLRTIYEQLAERLSSSYSLIYQSDRKVPDGTLRPVRISYRGSK
                     KSAGETAVFIPGMVVPSGGWSPLFLSMLGALTVLIVLPGLLARRRARV"
     gene            complement(3285..>3732)
                     /locus_tag="VHS97_00650"
     CDS             complement(3285..>3732)
                     /locus_tag="VHS97_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446730.1"
                     /translation="REQLGRSMAGLRPGVRVALASAGAVALRFVAVLLNALPFGGAPG
                     RLIEPRLSAIAFVLVAVGALAGLRLAIQLGEKRDLGSATIAGGALGFLGAALWYAMLQ
                     SFERLLGPWSTSIWAVAFLWGTFGALVGLVSIFMVPYRSKEPEVAR"
BASE COUNT          713 a         1325 c         1083 g          611 t
ORIGIN      
        1 gcttgatcgc ccagagcaac ggagccaagt ttatcgcgct gtcggcggtg ttagccggcg
       61 tgcgagaagt tcgtgcggcc atcgacgaag caaagaagag ccagcgccgc gatgctcgta
      121 ccatactctt catcgacgag atccatcgct tcaacaaggc ccagcaagac gccctgcttg
      181 gggcggtcga agacggcact gtcagcttga tcggcgccac cactgagaac ccgtcgttcg
      241 aggtcaactc ggcgttgctc tcgcgctgcc gcgtggtcat tctcgagccg cttggcccgg
      301 acgacgtcgc cacaatcatc cgccgggcgc tggtcgactc cgaacgcggg ctggcgggac
      361 gacgacccca tctcactccc gacttgatcg ataagctcgc gagctggtcg gccggcgacg
      421 cgcgcgtggc cctctcgact ttggaagatg ccgttcttgc cactgcgcct gccgaggacg
      481 gaacacgacc ggtcgccgag tcgacgctgg tcgaggcact gggacgagcg cggtatgcat
      541 acgaccggca aggagaagac cactacaacc tggcctcggc tctgatcaag tcggtgcgca
      601 actccgacgc cagcgccgcc ttgtactggt tggcgcggat gatcgagggg ggcgccgatc
      661 cgattttcat cgcgcgccgg ctctgcattc tcgcctccga ggacattggg ctggccgatc
      721 cgcaagcgat cgtccaggcg gccgcggcgg cccagatcac acacttgatc ggcttgcccg
      781 aggcactttt ccccctggcc caggccacaa tctatctggc gcaagcgccc aagtccaaca
      841 aggtgatgac cgcctacacc gccgccgcca ccgatgctgc tgcctcggcc cgcgaaccgg
      901 tcccactcca ccttcgcaat gcggtcacct cgctgatgaa aggcatcggc tacggcggcg
      961 gttaccgata catccacgac gatccggccg cccaaactga gaggcgctgc ctaccggagc
     1021 ggtttgaaga ccggaattat cttgaatagt tggcggtaaa atcgactgac gatccctggg
     1081 tcctggtgac cgagatcaaa cccgtgctct tcgtcgtgcc agcaggcccg gcaggacaat
     1141 cagcacggtg agagcaccaa gcatgctgag aaagagtggc gaccacccgc cggagggcac
     1201 caccattcct ggaataaaaa ccgccgtctc gcctgcggac tttttgctgc cgcgatagct
     1261 gatccgaacc ggccggagcg tcccgtcggg gactttgcgg tcgctctgat agatcagcga
     1321 atagctcgaa ctgagccgct ccgcgagttg ttcgtatatt gtccgaagct gatcggcgtg
     1381 acgtgccggg tagtactgcc cgcgtgtcga gacggcaagg cggcggagat cagcgctttc
     1441 gatctcttcc tcgctcccca ggcccagggt gtaaaccggc aaaccgattc gggccgcccc
     1501 cgcaatcacc gactcgaggt tcgccgattg actgaacgtg tcttccccgt ctgtcagcgc
     1561 caggacggcg cggcgccccg tctcatggtc gagcaattcg agcgcagccg cgaccgcgtc
     1621 gtaaaaacgc gtcgcccccg ccggctcgag ggcgtcaacg gccgtcttga tctggccccg
     1681 atccgtcgtg aacgaagcga gccggtcgac ctctgaccca aacgcgatca acgcgatcct
     1741 cgaaccctcg ggcagctttt ccaggaacga gcggaccgcc cgcttcaagc cgccgatccg
     1801 atcttcttcc tccatgctca ggctgtggtc gaccaccagg accacggtcg tgggaatcgc
     1861 ctcggtcgtc aggggcgcac ggaactcgac gacgtccact tcgcgccctt cttcggtcac
     1921 gccgaagtcg tcacgtcccg cgtcgagcag aaacgatccg tcgggtcgct tcacctcgaa
     1981 ctggaccgat atccgcggga acgcctgctc gctggcagcg gtcacattta cacgcacatc
     2041 tcccgcgccc gcctcgcccg tggtctcggc agcaggagag acattagccg caggcgtgga
     2101 atcggccgca ccgggcgcct ggccatccga ctcagcggaa cccggcccac tggtcttgac
     2161 gacatgaaga tcattccccg agcccggcag acggagcacc cgatcgattt cgaacgcaag
     2221 attggccttg cgcaagaaga gcaatgcgag gcatgtcgct ggcagcctgc cccattgacc
     2281 gtggggccag ccaccgtcat cttgctgcag ctcgatcagg atccgcgcgc cgtgagcgta
     2341 ccagtcgaat ccatcgagcg tgcgcaaacc cagcgccacg cagacccgct cgagcgacca
     2401 caggtaatag atgtcggaat gcagaccagc gcggcgggcg tcctggccga ccgccttcaa
     2461 tgccgactga aatgcgggat cggcggccag catggctccc cgagcgcgcg ccgtctgccg
     2521 ctcggcaagg ctcggccgcg acgcagcaat cgtcagaccc atcagtcccg cgcacgacat
     2581 cgagtcctta ccattcatgc caatccgata accccagcgg ccatcccgga gctgcgacga
     2641 ccgaaagtga tgatcaatcg cctcgagcgc ttgatccgaa tcgtatccgt gccggcttgc
     2701 cgcccacaga cccaagagag cgaactgcgt attggaattg tccccttgac tcgggaaaaa
     2761 ctgaggccgg cgcgcgcccc tcttgcccgc ggcaccgcgc gatcgggtct tggcaggcac
     2821 cggctccagt tcgctccgcg gcacctggta atcccatatc ccttcgcgat ctccccccgc
     2881 caggcgctgc cccagcgttc ggataagcgc atcggcctcg cctcgccgcc cttgctggca
     2941 ccgcgccaga aacaagatcg ccagcgcaat gtcgtacgtt tgatcggaag tcgtcgccat
     3001 ctcgctaaca atctcgcgcg cccgactgat ctcgggcgcg tcgcgagcca ctccattctc
     3061 catgagcgcc agacccgcca gcgctgtcat gcccagacgg tgttcgtttt gcgcatgctc
     3121 gtcccagtgc ccctggggcg actgcgcgtc cttcaaaaac gccactcccc gcgcgatcgt
     3181 cgcggcgatc tctttgtcct cgggcacgtc ggcatgggcc cgccccgagc ccacgacgca
     3241 caccagaatc gccaagctcg cgcccctggc tttcatccgc gtgctcatcg cgcgacctcc
     3301 ggctctttgg accgatacgg gaccatgaat atcgagacca gaccgaccaa agccccgaac
     3361 gttccccaca aaaaagcgac ggcccagatc gacgtcgacc acggacccag caaacgctcg
     3421 aagctctgga gcatcgcata ccacaatgcg gcccccaaaa accccagcgc accccctgca
     3481 atcgtggcag aaccaaggtc gcgcttttca ccaagctgga tcgccagcct cagccccgcc
     3541 agagcgccga cggcgacgag cacaaacgca atcgccgaca gccgcggctc tatcaggcga
     3601 cccggcgcgc cgccgaacgg tagtgcgttc aggaggacgg caacgaacct tagagcgacc
     3661 gcgccggcag atgccagcgc gactcgcacg ccgggccgca gaccagccat cgatcgccca
     3721 agttgttccc gc
//
LOCUS       DATAQL010000039         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1683221, whole genome shotgun sequence.
ACCESSION   DATAQL010000039 DATAQL010000000
VERSION     DATAQL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683221"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..813
                     /locus_tag="VHS97_00655"
     CDS             400..813
                     /locus_tag="VHS97_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3446731.1"
                     /translation="MDKIKAGLSDAEREALRVLWDHGPGTVRRLMELLVERGHRWAYS
                     TVATLLRRLEGKGYSASESVGGSLVYRATLSREDLLERRLKDAAVELCDGAEAPLVFL
                     ALVQGNRFTTAELDRIRQIIDDAKKREGRSPKPGR"
     gene            863..>2537
                     /locus_tag="VHS97_00660"
     CDS             863..>2537
                     /locus_tag="VHS97_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3446732.1"
                     /translation="MLVWFAETSLIAVVLAAVAMVAGRWTRLGPGARHALWLVVMIKL
                     VTPPVVSWPWAVSLASPPHEVAASPEAKGRASKDVLGASLDASRSKDLSLPVTTHLLV
                     VPAAWADSSLASRGTLGVWCLASAALALWHISRVACFRRRLGWALPAPDWLVEQALCV
                     AHRLGVRAPEILVVPGLTTPMLWFLGRPKLLVPAGLVKTLGSVGWQGILAHELAHLSR
                     RDHWVRRVELAAGLFWWWNPLYWLTRGRLEAEAELACDEWAVRVFPEGRLDYAEALLQ
                     VCKSHCLDRPPEPALGIAGAGRFLERRVTMILRDQIPFRASRPVLIGAGLLALVALPS
                     WSASATSDPDQDKTDQSAASPVATAGEIVSSGDDKQVTWPANGADSRPADRATNSRLP
                     RRTGRQTSDNVIAPPANDLGDVRPDHVRRGRNSADARPDEQETRSRQLRRQGPVSTRP
                     ALPPNNVHRGDARPAIADVDSASPDARPNGDPTPRRASQDVNDARIAGQRGGQIGLDV
                     QPGKKTIGTSVGRDRQKAAASESRGREQRTRSRRIREMRALIDQLQNELE"
BASE COUNT          462 a          752 c          861 g          462 t
ORIGIN      
        1 gctcgtctac tgcggcgccg gcatgagtcg ctcgccctac atcgcggcgg cggcaatcgc
       61 gcttgtgcgc acacacgctt tcgacgacgc gctcaaactc gtgtccaact cggggccggt
      121 cgacgtgtcg cctgacctat gggcagacgt tcgagtggtt ctgggttgat ccagtttcct
      181 ggatcattcc ttaaaaccgt cggaagcccg ccacacatcc gccgcatacc gttactcacg
      241 gcgcgaggca agtcgttggt cgaacagcct tcgtgattcc gccgatcatc ccctaactat
      301 gcggattacg caaaaatgac ttgacggctg ttacaagttg taataggatg cctgttacaa
      361 ggtgtaactg gaaatgggga cgacgacccg aggatgagga tggacaagat caaggccggg
      421 ctcagtgacg cggagcgcga ggcactgcgg gttctttggg atcatggtcc cgggacggtc
      481 cgtcggctca tggagctgct cgtggagcgc ggccatcgat gggcgtattc gaccgtggcg
      541 acgctgcttc ggcgactgga gggcaaaggt tacagcgcga gcgagagcgt gggcggatca
      601 ttggtatatc gcgccacgct ctcgcgggaa gacctgcttg agcgacggct caaggacgcg
      661 gcggtcgaac tgtgcgatgg ggcggaggcc ccgctcgtat tcttggccct ggtacagggg
      721 aatcggttta cgaccgctga gctcgatcga atccgccaga tcattgacga tgcgaagaag
      781 agagaaggcc gttcgcccaa gccgggcaga tagacagcgc agcgttgtgt tggtttgatg
      841 tgcgaggtgg ggagacgtcg tgatgctcgt ctggttcgcg gagacatcct tgattgctgt
      901 ggtcctggca gccgtggcga tggtcgctgg ccgttggacc cggctcggac ccggggcgcg
      961 acacgccctc tggctagtgg tcatgatcaa gctcgtgact ccgccggtcg tgagctggcc
     1021 ctgggctgtc agcctggcaa gtccgcccca cgaagtggcc gcgtcgccgg aggcaaaggg
     1081 ccgcgcgtcg aaagacgttc taggtgcatc gctcgacgcc agtcggtcga aggatctgtc
     1141 gctgccggtc acaacacatc tcctagtggt accggcggcg tgggcggatt cctcgctggc
     1201 cagccgaggg acgttaggag tctggtgcct cgcgtcggcg gcgctggcgc tttggcacat
     1261 tagtcgggtc gcgtgcttcc gtcgtcgact cggatgggcg ttaccggcgc cagactggct
     1321 cgtggagcag gccctgtgcg tcgcgcatcg gcttggcgtg cgtgcacccg agattcttgt
     1381 cgttcccggt ttgacgacgc ccatgctctg gtttctgggc cgtccgaagc tgcttgtgcc
     1441 ggcgggtctt gtcaaaacgc tcgggtcagt cggatggcag gggattctgg cacacgagct
     1501 cgcccacttg tcgcgccgcg accactgggt acgccgcgtc gagctggccg ccggcctttt
     1561 ttggtggtgg aacccgcttt actggttgac gcggggtcgg ctcgaggccg aggccgaact
     1621 ggcctgcgac gagtgggccg tgcgtgtttt ccccgaaggt cgtcttgatt acgccgaagc
     1681 attgctccaa gtctgcaaat ctcattgcct tgacaggccg ccggaacccg cgttgggcat
     1741 cgccggggct ggtcgatttc tcgagaggag agtgacgatg atcctgcgtg accaaatccc
     1801 attccgcgct tcacggccgg tgctcatcgg cgcgggccta ttggcccttg tggcactgcc
     1861 cagctggagc gcgtccgcga cctcggaccc ggatcaagat aagacagacc aatcggccgc
     1921 cagccccgtt gcgactgccg gcgaaatcgt gtcgtcgggc gacgacaaac aggttacatg
     1981 gccggcgaac ggcgccgact ccagaccagc cgatcgcgcg acgaactcac ggctgccgcg
     2041 tcgcaccggc cgacaaacaa gcgacaacgt gatcgctccg cccgcaaacg acctagggga
     2101 cgtgcggccg gatcatgttc gaagaggtcg gaacagtgcc gacgcgagac cagacgagca
     2161 agagacacga tcaaggcagt tgcgtcgaca aggtcccgta tcgactcggc cagcgcttcc
     2221 accgaacaat gtccacagag gggacgcccg tcccgcgatc gctgacgtgg attcggccag
     2281 tcccgacgcg agaccgaacg gggatccgac gcccagaagg gcatcgcaag atgtgaacga
     2341 tgcgcgcatt gccggacaga ggggagggca gatcggactc gatgttcagc ccgggaagaa
     2401 aacgatcggt acctctgtag gacgggaccg ccagaaagct gcagcctccg agagcagggg
     2461 gcgggagcag cggacaaggt cccggcgcat ccgggaaatg agggcgctga tcgaccagct
     2521 ccagaatgag ctcgagg
//
LOCUS       DATAQL010000040         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_308989, whole genome shotgun sequence.
ACCESSION   DATAQL010000040 DATAQL010000000
VERSION     DATAQL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308989"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..657
                     /locus_tag="VHS97_00665"
                     /pseudo
     CDS             1..657
                     /locus_tag="VHS97_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(883..1522)
                     /locus_tag="VHS97_00670"
                     /pseudo
     CDS             complement(883..1522)
                     /locus_tag="VHS97_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          300 a          476 c          469 g          277 t
ORIGIN      
        1 gatgccgacc tcgaagcggt cgtcgagggc gttagggtct tcggctacta caacgccggt
       61 caggactgca cggctgcctg ccgagtctac gcgggcaaca aagtttacga caggcttgtc
      121 gcggatctcg ccgcggcggc ccaaaccctc aagttcaacc agcccaacga cgacgagaac
      181 gatttcggtc ccctgatttc ggggcgtcag cgctcgcgtg tttcgtagtt cgtcgagagg
      241 gccctcgagt cgaagcatgt gtcgatcgcc gccggcggcc agcagacgaa aggcaagggc
      301 ttcttttacg agccgaccgt cgtcgccggg gcgctgcagt ccgacgagat cgtccggcga
      361 gaagttttcg gccctgtcgt ctcggtgacc cggttcgacg acgccgaaca ggccatcgcc
      421 tgggccaacg actccgacta tggcctcgcg tcgtcggttt ggaccaggga cgtgtccaag
      481 ggcatgcaga cggccgcgcg cctccaatac tgctgcacct gggtcaactg ccatttcatg
      541 ctgacgaacg agatgccgca cggcggcgtc aagtcctccg gctacggcaa ggatctgtcg
      601 agctatgccc tggaggacta cacgttcgtt cggcacgtga tggtgaagtc gagctgaggc
      661 gcgcaatccg cctcacgccc tgacgcgcag tccctcggga tcgtagggcg gcttcaggaa
      721 gggtcttgcg gggcgctgtc agaccaacgg tagatcgggg cgagcgacgc atccaatcgg
      781 aaagccgatg tgcccgagcg ggaaggagct gaggaaacgc cgcggacttg tcacgagaac
      841 ttggctttgg ccaaacgaaa gtcagaaggg ctcggctcga gttcacgccg cagtcgctgc
      901 ccgccagttc tgcaacgctt cgcctctgag gacgcgaagc gtgtcgcgag aaacaagatg
      961 acgctgaacg ttgaacgtat tctggacgat ggcgtgagca gacaggaacc gttgcgctga
     1021 tccgggcgat ttgaacgctg catcttccgc tcgcgccgtc gcaccggcag atgcgaattc
     1081 tcggccctat tgttcttgcg caggccttgc tcatggcgag ccgataagcc cagttgcgcc
     1141 ttgaccgctc cataggagcg cagcttgtcg gtaacaagcg tcttgggcgc gaagccctgc
     1201 ttcttaagca gtctgcgcat gagcttccca gccgccttgg cgtcgcgccg ccgttgcacc
     1261 agcaggtcga gaacctcgcc ctcgtcatcg actgcccgcc agagccagaa tcgtttaccg
     1321 ccgatcagga ccgccatctc gtcgagatgc cactgggaag ttggccgatg acgtctgcga
     1381 cgaagttcct tggcaaacaa cggcccgaac ttcagcaccc atcgcctgac tgtctcatag
     1441 agaattccaa cccacgctcg gccagcaaat cctcgacatc gcggaagctg agagtgaagc
     1501 gaaggtacaa ccacaccgca tg
//
LOCUS       DATAQL010000041         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4125752, whole genome shotgun sequence.
ACCESSION   DATAQL010000041 DATAQL010000000
VERSION     DATAQL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4125752"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VHS97_00675"
     CDS             <1..653
                     /locus_tag="VHS97_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446733.1"
                     /translation="MIGLLTKWRRAERALCRQLGRGASFNEVADALGLTEAQKMLVAK
                     AHQARQLKLENNITAPAHRGLSWGSADHNTPTEAMLEANDEWRILLDRLGRLEPRERS
                     VLVLRYGLEGESALTLKEIGRRLGVTREWVRKIEIRAVRKLHNEYAAEMSGPPGGGSS
                     TRPARRFGRAQTEEVVMSGSPSARTGARPRSRARAQSSRRSTRRGQSPALAVTSAL"
     gene            840..2435
                     /locus_tag="VHS97_00680"
     CDS             840..2435
                     /locus_tag="VHS97_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446734.1"
                     /translation="MPQSRRRRGVFAESAIAACLVALVSAVNIGFNTVWSAPPRFDGA
                     GYAVLAQSWLRGEGYRAIDHPDRPRHAHFPPGYPLILALTWGLAGKSAAAGHAVSSLL
                     TVGATLAAWLWFRSFLPGPTALALGLALAVNWIWARTGSAIQSEPLYLFLSHATLLAA
                     VTAGRRTALGTWRSIALGALLAACVLTRQVALGLVAAVVIDLVARHRFADAGTALGSA
                     ALFVFPWVAWIATIGPAAKTQAGLLVGGDGTWTERLGRQSVFYLQRIPDQLTGPFVEV
                     GTDLGRGPIIGIAANLWAAMVTGLIIAGWIRCMGRPRRRLAGLLPLVTLAMLAIWPYT
                     EAGRFLIPLIPCLLIGAIEGTVGLATLLCRLSGSRMRVARIRLYAASLILMASLPYSA
                     YLLATGRARALESTHRAFDLACAWIAAHGDRPGPLISRHPGEVFWQTGRQGLEVTSVE
                     RPGDRDADPDTIARLIAAYHVSYLLIDQHRYAGAPRSPLAQFVAERPELAREIWRSNS
                     GPDSVIIYEVSAGGPGGTPDASL"
     gene            complement(2446..>3215)
                     /locus_tag="VHS97_00685"
     CDS             complement(2446..>3215)
                     /locus_tag="VHS97_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEX3446735.1"
                     /translation="IGLGAWPAPPQEAGQLKVKRTIPPEVVEDDIVTRRAVCRWAAQP
                     PVLDGKLDDRCWQKGAVIDHFASYWTKTPRQGTFAYLVWDDDALYYAGSMTDAELRSY
                     GTKRNDTLWDGDVFELFFKPSAEKPEYFEFQANPRMLVFEMAFPKRGDYGGKFSEAPI
                     LGNKAVVALDGTLDQPGDRDLGWTVEGRIPWSAFANGKAKPKPGDEWFFALCRYDYGP
                     QGTKPVLMSSAPLTQPSFHRYEDYGKLRFEGPRERGR"
BASE COUNT          547 a         1002 c         1004 g          662 t
ORIGIN      
        1 acatgatcgg tctattgacc aagtggcgcc gggccgagcg ggctctctgc cggcaactgg
       61 gccgcggggc tagctttaac gaagtagccg acgcactcgg tctcaccgag gcccaaaaaa
      121 tgctcgtggc caaggcgcac caggctcgcc agctcaagct ggagaacaac atcacggcgc
      181 cggctcaccg gggattgtcg tggggatccg ccgaccataa cacgccaacc gaggcgatgc
      241 tggaagcgaa cgacgagtgg cgcatcctgc tagaccggct gggtcgtctg gagccccgcg
      301 agcggtccgt cctggttctt cggtacggac tggaaggcga atcggcccta acactcaagg
      361 agatcggccg caggcttgga gtaacccgag aatgggtgcg caagatcgag atccgtgccg
      421 tacgaaaact tcacaacgaa tacgctgccg aaatgtccgg gcctcctggc ggcgggagct
      481 cgactcgtcc cgcccggcgt ttcggtagag cacaaaccga ggaagtcgtc atgagcggct
      541 ctccgtccgc caggacaggc gccaggccca ggtcacgagc tcgagcgcag tcgtcgcgtc
      601 gttcgacccg tcgcggtcaa tcgccggcgc tggcggtcac gtcggctcta tgacgttgac
      661 ccgtatgcaa ttgccgtgtg cacaaaccgc cggcgctctc cagcgtgtct aggcgtacgc
      721 cagcattgtg ccttgcggtg tccgatcccc ggatgctcca atcactttgg cttcgtggga
      781 tctggagcat caacgtattg acccgctcgc gccatcgagc gcgctggaga ctgagcgcga
      841 tgccgcaatc gcgaaggcgg cgcggagtat tcgcagagag tgccatcgcg gcctgcttgg
      901 ttgccctcgt gtcggctgtc aatatcggct tcaacactgt ttggtcggct ccgccgcggt
      961 tcgacggagc tggctacgcc gtgctcgcgc aatcttggct tcggggagaa ggctaccggg
     1021 ccatcgacca tcccgatcga ccccggcatg cgcattttcc cccgggttat cccctcattt
     1081 tggcactgac atgggggctc gcgggcaagt cggccgcggc gggacacgcc gtgtcgagcc
     1141 ttctcaccgt gggggcaact ctcgcggcct ggctctggtt ccgcagtttc ttgcccggac
     1201 cgacagcgct ggctctggga ctggctctag cggttaactg gatctgggcg cgaaccggaa
     1261 gcgccatcca gtcggagcct ctctatttgt ttctgagcca cgccaccctc cttgcggccg
     1321 ttaccgcggg acggcgaacc gcgctgggca cgtggcgatc gatcgccttg ggcgcgcttt
     1381 tggccgcttg cgtgctcact cgccaggttg ctttgggctt ggtcgctgcg gttgtcatcg
     1441 accttgttgc tcgtcaccgg tttgcggacg cgggaacagc tctaggttcg gcggccttat
     1501 tcgtcttccc gtgggtggct tggatcgcca cgatcgggcc agcggccaaa acgcaggccg
     1561 gcctgctcgt cggaggggat gggacatgga ccgagcgatt aggacgacaa tcggtctttt
     1621 acctccagcg catccccgac cagctcaccg gtccgttcgt cgaggtcggt accgacttag
     1681 ggagggggcc gatcattggc atcgcggcca atctttgggc ggcgatggtt accggattga
     1741 tcattgcggg ctggatccgc tgcatggggc ggccacgacg ccgtttggcc ggccttcttc
     1801 ccctggtcac cttggctatg ctcgcaatct ggccctatac cgaggcagga cggtttctta
     1861 tcccgcttat cccgtgcctt ttgatcggcg caattgaggg aaccgtgggc ttggcgactc
     1921 tattgtgtcg actgagcggc agcaggatgc gagtcgcccg gatcaggctg tacgcggcga
     1981 gccttatcct gatggcatcg cttccctatt cggcgtactt gctggcgacg ggccgcgccc
     2041 gtgctctcga gtcaacccat cgcgccttcg acctagcgtg tgcctggatc gccgcacatg
     2101 gggatcggcc cggcccgttg atatcgcgcc atcctggcga agtcttctgg caaactggtc
     2161 gtcaagggct cgaggtgaca tccgtggaac gacccggcga ccgcgatgcg gatcccgaca
     2221 cgatcgcccg gttgatcgcg gcctaccacg tttcgtattt actgatcgac cagcatcgct
     2281 acgccggagc acctcgtagc ccgctggctc agttcgtcgc tgagcgcccg gagctcgctc
     2341 gtgaaatatg gcgaagcaac tccggcccgg attccgtgat catttatgag gtgtcagcgg
     2401 gaggcccggg gggcacgcca gacgcatcct tgtagttggt tttgactagc ggccccgttc
     2461 acgcgggcct tcaaaccgaa gcttgccata atcttcgtaa cgatgaaagc tgggctgggt
     2521 caagggagcg gagctcatga gcaccggctt ggtcccttgc ggcccataat catagcggca
     2581 caatgcaaaa aaccattcgt cgccgggttt gggtttcgct ttcccatttg caaaggccga
     2641 ccacggaatt cggccttcga cggtccaacc gagatcgcga tcgccaggct gatcaagtgt
     2701 cccatcgagc gcgaccacgg ctttgtttcc caatatcggt gcctcgctaa atttgccgcc
     2761 gtaatccccg cgcttgggaa aggccatttc gaacaccagc atcctgggat tggcctgaaa
     2821 ctcgaagtat tcaggctttt cggcgctcgg tttgaaaaaa agctcgaaga cgtcaccgtc
     2881 ccagagagta tcgttgcgct tcgttccata agagcgaagc tcggcgtcgg tcattgagcc
     2941 ggcgtaataa agtgcatcat cgtcccagac caggtaggcg aacgttccct gtcggggggt
     3001 ctttgtccag taagacgcaa agtggtcgat cactgctcct ttttgccagc accggtcgtc
     3061 aagcttgccg tcgagcaccg gcggctgcgc ggcccatcga cagacggctc gccgggtcac
     3121 gatgtcgtct tcaaccacct cgggtggaat ggttcttttg accttgagct ggccggcctc
     3181 ttgtggcggc gccggccacg cgcccaagcc gattg
//
LOCUS       DATAQL010000042         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4125981, whole genome shotgun sequence.
ACCESSION   DATAQL010000042 DATAQL010000000
VERSION     DATAQL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4125981"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1235)
                     /locus_tag="VHS97_00690"
     CDS             complement(270..1235)
                     /locus_tag="VHS97_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446736.1"
                     /translation="MRFCETGMPLEDRPIDFDADGRFVRLKAAGLFHLVDKAKPLGTC
                     HFGGERRPAGLVAGLVCRRHDQDRQGADPVAVRHDCLEPTPKIVQNRRRLDRTADRWV
                     DELMTEGKRGRTVQPVFADKLIGQVVEGRAQRGPVGDSRQPEACSLILPPLSLLFEAS
                     GELIVPRVDDDRIRTPGQPCQLFVLHLQRISRIGQDLARETTVGDRLLQLPGHDLAYR
                     QPRPVGLSHRARTTEDHNPVFAGGILDKSRRQRRKPMRPASPGRRTNLEHEERNQARQ
                     SEKEAALQSALELAASPWAGKERREHRRSYETGEKDRAVDDQYGL"
     gene            complement(1896..2465)
                     /locus_tag="VHS97_00695"
     CDS             complement(1896..2465)
                     /locus_tag="VHS97_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEX3446737.1"
                     /translation="MPILPAEPDCYPPDLWDNPASIGAKEEAVWWCLHTKPRQEKAIA
                     RDLRVQGIVYYLPQMLKESRTPQGRKIQSIVPLFTSYMFLNGNVNDRLAALRANRLVS
                     VIEVVDQASLERDLRQINTMLSSGLPVTAEEAAPVGATVRILTGPLTGMVGKVIRRAK
                     RDQFVAVVHFLGRGAMVDLQDWQVEQVLE"
BASE COUNT          527 a          855 c          864 g          560 t
ORIGIN      
        1 taacgaccgg gtctgtcagg cgccggccaa cctggaaatc ctattgacag taactgttgg
       61 gcaatgacat ctcccgacac agagggaccc tcttgagaaa gtcgttgtgt acggctgacg
      121 cggcggacga gggtcttcgg atctcgcggc tactgtatct ctgcgccatt gcccttccct
      181 ttgtcgttct catctcggcg gagctcgtaa ttccagggtg ggttcccacg ctgcccagac
      241 cgttccgtcg cgcgctgacg gaggcattat tacaaaccgt actggtcgtc tactgcgcga
      301 tccttctcgc cggtctcgta ggaacgccgg tgctcgcgtc gctccttgcc agcccatggc
      361 gacgcggcaa gttcgagcgc ggactgaagc gcggcttcct tttcggactg tcgtgcttgg
      421 tttcgctcct catgctcgag gttggttcgg cggcctggcg aagctggacg catcggcttc
      481 cgtcgctgcc gacgcgattt gtcgagaatc cccccggcga atacaggatt gtggtcctcg
      541 gtggttcgag cgcggtggga gagccctact ggccgtggtt gtcggtaggc cagatcgtgg
      601 cctggcaatt gcagcagacg atcgccgacc gtcgtttcgc gtgcgagatc ctggcctatc
      661 cgggagattc gctggagatg cagcacaaaa agctggcagg gctgacccgg cgtccgaatg
      721 cgatcatcat ctactcgggg cacaatgagt tcgccgctcg cttcgaagag gagcgagagg
      781 ggtggcagga tgagcgaaca ggcttctggc tgtcgcgagt cgcctacagg gcctctctga
      841 gctctccctt ctacaacttg gcctatgagc ttatcagcaa aaaccggctg gaccgtccgc
      901 cctcgctttc cctccgtcat cagctcatcg acccaccggt ctgcagtccg gtcgagtcgg
      961 cggcgattct ggacgatttt gggcgtcggc tcgaggcaat cgtggcgtac tgcgacggga
     1021 tcggcgcctt gccgatcttg atcgtgccgc cggcaaacga ggccggctac gagcccagcc
     1081 ggtcgacgct ccccgccgaa gtgacaagtt cccagcggct tcgccttgtc gacgagatgg
     1141 aagaggcccg cagccttgag tcgcacgaac cgaccgtcag cgtcaaaatc tatcggacga
     1201 tcctcgagcg gcatcccggt ttcgcagaag cgcatttccg gctggcacgt ctgctggaat
     1261 cacacgggca agtaaccgag gcagcgcctc actatcttgc cgcactcgat cacgacggat
     1321 tgccgattcg atgccaagca ccgtttcgcg cggtctatcg agcggtcgca gcgcggcatc
     1381 ctcgctccgt gctgatcgac ggaagacgcg agctcagcga gatcagtccc aatgggctgc
     1441 ttggtgacca tgtgatccac gacacgcatc accccacatt gcggggccat gtggcgcttg
     1501 ccggcgcggt cctccgcgag ctggcgcgtg tgaacgtctt cggcttgacc gtcccgatca
     1561 acctgccact cgatccagcc gctagtgccg aacactttgg cataaacgct gaaaggtggg
     1621 cgacaacgtg tgaaaggatt agtgaacact acaggcgcgt ggcgatctat cgctacgatc
     1681 cggcagagag gctgcgaaag gctcaagttt ttgccgaagc tgctcgccgg atacgcggcg
     1741 gttgcttgcc cggtcaactc ggtctgccgg gaattggatg cgagcgaagc gcaaaagacc
     1801 gattgcttgg ggaatcacct gatactattc ccgatgcgcc ggccgaaccc tcaaagcagg
     1861 cggcgatcac gcatcgagcg acccgcgcgg atggttcatt cgaggacctg ttccacctgc
     1921 cagtcctgca ggtcgaccat cgcgccgcgg cccaagaaat ggaccacggc gacgaactgg
     1981 tcgcgtttgg cccggcgaat caccttgcca accatacccg tgagcggtcc ggtcaagatc
     2041 cgaaccgtag cgccgaccgg tgcggcctct tcggcggtga ccggcaagcc cgagctcaac
     2101 atcgtattga tctggcggag atcgcgctcc agcgatgcct gatcaacgac ctcgatcacg
     2161 ctcaccaggc ggttggctct gagggctgcg agccgatcgt tgacattgcc gttcaggaac
     2221 atgtaactgg tgaagagcgg gacaatcgat tgaatctttc gcccctgggg tgtgcgactt
     2281 tccttgagca tctgaggaag gtaatagaca attccctgaa ctcgcaggtc gcgcgcgatc
     2341 gccttttcct ggcggggctt ggtgtgcaga caccaccaga ccgcttcttc cttggccccg
     2401 atcgacgcgg gattatccca aaggtcaggt gggtaacagt cgggctcggc tggcaagatc
     2461 ggcattacgg agctcggaga tgaaccatcg cgggctgcaa tggccgccgc gattttcgat
     2521 caaactgtct gcagttttca gcccaactta gttaaacgcg cgagcggcgt caagcgagac
     2581 gaacggttga cggcgggccg tgatagtgag ctggctatag cgagcctgga gacatgcaat
     2641 gaaagccgat caacggccgg gaacagggaa ttacgcaggg cttactggcg tccttgttct
     2701 caataagcca tcgggcatta cctcccgcaa gctcgtcgac caggtcgcgc ggctggcatc
     2761 gcgttcgaag gtggggcacg cgggcaccct cgatcccctg gcgacc
//
LOCUS       DATAQL010000043         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4278616, whole genome shotgun sequence.
ACCESSION   DATAQL010000043 DATAQL010000000
VERSION     DATAQL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4278616"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..833)
                     /locus_tag="VHS97_00700"
     CDS             complement(273..833)
                     /locus_tag="VHS97_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446738.1"
                     /translation="MNDVTRILSAIEQRDPKAPEQLLPLVYEELRKLAAQRLARESPG
                     QTLQATALVHDAYLRLVGSEAPSWNSRGHFFSAAAEAMRRILIENARRKRAVKRGGGL
                     KRVEFDGMDMPEAALAHDILAVDEALSKLAAEDPAKAELVKLRYFGGLSVEDAGRVLG
                     ISRATADRYWAYARVWLYSELSEGEQ"
     gene            1255..1617
                     /locus_tag="VHS97_00705"
     CDS             1255..1617
                     /locus_tag="VHS97_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3446739.1"
                     /translation="MSSFSTRSDSNVALVTFENASGLNDFRNTALRDGLYELVATREE
                     PLLAIDLAKVDYLSSSGVAILVGLKRRVETKGGKIVIFHVQPIVRDLLAVMKLDRFFL
                     IADDESKAVASVRPLPPA"
     gene            1936..>2939
                     /locus_tag="VHS97_00710"
     CDS             1936..>2939
                     /locus_tag="VHS97_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446740.1"
                     /translation="MSFMQQRSGRSDWARRRLVSAPEALESRQVLSSGVPAYASPWLP
                     SDLFVTNPITHQRELVNSSQLVHQNDPNSPGLSNQGKIVTGQDRQGDKWTITVHGPGE
                     VIVTDTTPNDGNLGDDIDTIQLINTNPRTTFVTGNVIPSNTFPRIDTTSLQDITFTTP
                     NIQGNASGTPLEPSSGVIKFNQLIDLNGVKSIELNGFSLDANVSPAVTSQTGIFLYGG
                     VGVLSFDNIDANINTAVNSTPYQIVIGEPNTPLKVEPSIFINHVFNLVFNGATQSSPP
                     TTPVTSPSVEFMINGVTRSFDMIASSQPKIPAGLEVQFPPVGTTGRTSIQATAVNTLT
                     V"
BASE COUNT          588 a          935 c          827 g          589 t
ORIGIN      
        1 ggcttctagt ggttgtccgg cccatccagg gcgagatttt ttgatctgtt gcgggcttcc
       61 cagggcgttg ccctgggcta gcatgtgact ggcccgtcgg gccgaatcgc aggatgctca
      121 tcccccaggc tggcacctga aattttcctg gcacaacttc tacgtgtacg ctcgaccaac
      181 gcgctcacgc ttccctcact gcctactgcc taccgccttc cgactaatga ctaatgacca
      241 atgaccaatg actaatgacc aatgaccaat gactactgct cgccttcgct cagttcgctg
      301 taaagccaga cacgcgcata tgcccagtag cgatccgccg tcgcgcgtga gatccccagc
      361 acgcggccgg cgtcctcaac cgaaagaccg ccgaaatagc ggagcttgac cagctcggcc
      421 ttagcggggt cttcggccgc gagcttggaa agggcctcgt caaccgcgag gatgtcgtgg
      481 gccaaggcgg cctccggcat gtccattccg tcgaattcga ctcgcttcaa gcctccgccg
      541 cgcttgacgg cccgcttgcg ccgggcgttc tcaatcagga tgcggcgcat ggcctcggcg
      601 gcggccgaga agaaatgccc ccgactgttc caggagggtg cctcagagcc taccaagcgc
      661 agataggcat cgtgaaccag ggccgtggcc tggagcgtct ggcccggaga ctcgcgggcc
      721 agccgttggg cggccagctt acgcagctcc tcatagacca gagggagcag ttgctctggc
      781 gccttggggt ctcgttgctc gatcgccgag aggatgcggg tgacgtcgtt catcgacata
      841 aactctatca accgccccgg tgacactgtg gaccctggcg cgagcgtcat cgctcggcga
      901 gatcccatgt gcgccggcat gacagccgga gcggttgagc ccatcgtaac ccgcgagtgt
      961 gggccgggat agccacctcc aacgaagagt aagatcaaga tgaagattat gatgatgaag
     1021 acgattaaga gcaggatcaa gattaagagt aggacccaat ctccttggac ccgttctcgt
     1081 cgcccaaaac tgcctgctgc ctacaaccta cttgcttcga tgggatgagt ttcgagcacg
     1141 taatctccgg gtgaattgca acgaccttga tcggcgcgat atgatggagg tgcgccgtaa
     1201 ggcgggctgc gctacggcat tctggatcgc ctcggtggag gaactctcga cgtcatgtca
     1261 tcattcagca ctcgctcgga ctcgaacgtc gcactcgtga cgttcgagaa cgcctctggc
     1321 ctgaacgatt ttcgcaatac ggcgctccgc gacggactct acgaactggt cgcaacgcgc
     1381 gaagaaccgc ttctggccat cgacctcgcc aaggtcgact acctttccag ctctggcgtc
     1441 gccatcctcg ttggcctcaa acgccgcgtc gaaaccaaag gcggcaagat cgtcatcttc
     1501 cacgttcaac cgatcgtccg cgacctcctc gccgtcatga agctcgaccg gttcttcttg
     1561 atcgccgacg acgaaagcaa ggccgtcgcc tccgttcgtc cccttcctcc ggcctaatcc
     1621 gtctcttcgc tttcctcgat accctgtatt tcccatcttt acagcagccg tcaagacagg
     1681 ggccgccgtg cgccgattga gagaggcgta agtgagtcgt tgcgccgaca gtgaatggag
     1741 tgaggtggcg aggctcgttc cggcaggatc ctcgacaaat aacggtgtcg cgcggcggac
     1801 ttgcggtttc tccagagccg gttgaccatc cgaaccaagg ggagggcggc tgcaagggag
     1861 ccggttcgat tgttcctgct ggaacgatcg acagattcgg cttgccctgg cgagctgccg
     1921 ggaagggagt cttccatgag tttcatgcag cagcggtctg gccgctccga ttgggctcgg
     1981 cgcaggctgg tctcggcacc ggaggccctt gagagcaggc aggtgctctc gagcggggtt
     2041 ccggcatacg cttcgccttg gctacccagc gacctttttg tcaccaatcc gatcacgcat
     2101 cagcgcgagc tggtcaactc ttcccagctc gtccaccaga acgatcccaa cagcccgggg
     2161 ctctccaacc agggcaagat tgtcaccggc caggatcgcc aaggcgacaa gtggaccatc
     2221 acggttcacg ggccgggcga agtgatcgtg accgacacca cgcccaacga cggcaacctc
     2281 ggcgacgaca tcgacacgat tcagctcatc aacaccaacc cgcgtacgac ctttgtcacc
     2341 ggcaatgtga tcccgtcgaa cacctttccg cggattgaca ccaccagtct gcaagacatc
     2401 accttcacga cccctaacat tcagggcaat gccagcggta cccccctcga gcccagcagc
     2461 ggcgtcatca aattcaatca gctcatcgac ctaaacggtg tgaagtcgat cgagcttaac
     2521 ggcttttctc tcgacgccaa tgtctcgccg gcggtgacca gccagaccgg tatcttcctc
     2581 tacggcggcg tcggggtgct ctcgtttgat aacatagatg ctaatatcaa tacagccgtc
     2641 aactcgacgc cgtatcagat cgtgattggc gagccgaata ctccgctcaa ggttgagccg
     2701 tcgattttca tcaaccatgt tttcaatttg gtctttaacg gcgcgacgca atcgagtccg
     2761 ccgaccacgc cagtgacgtc tccgtcggtc gagtttatga tcaacggggt gacccgcagc
     2821 ttcgacatga tcgcgtcgtc gcagccgaag attcccgccg gtctggaggt tcaattcccg
     2881 cccgttggga ccactggccg aacgtcaatc caggcgactg cggtcaacac gctcacggt
//
LOCUS       DATAQL010000044         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2446902, whole genome shotgun sequence.
ACCESSION   DATAQL010000044 DATAQL010000000
VERSION     DATAQL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446902"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2013
                     /locus_tag="VHS97_00715"
     CDS             <1..2013
                     /locus_tag="VHS97_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446741.1"
                     /translation="EDLLAEAEPKDRPAILELLRKSYDVMARDAKAAGRDREATHYRD
                     NLAIIEHCRDANAPAKPPDAKPDKPAAPGPLTDRDKSASGTAAPQPRPHSPKVLLPGS
                     VQDSAPSIQAAVAPALEPPMSPIVDVSAASPRPVDTPNAPPAGPTAASSSPPSINIQP
                     ARNEADATVAAPTSSEPTGSAGPSSTRAAAGAVQPANTPNESAGPSLADGDRLFASGR
                     YEDAGRCYAALAAQNGLPARRQAHWAYCRMVNVAQRINARPKTPREWDEIEAEIVKIQ
                     RLAPNNWYGEYLRNKVAEVRRGARRTQPKSDNLVVRGRAPDDSRNKPDPEPRRFSRLF
                     GKSRAPTPAPGQPSGALAANNAEMPLNLPQESQPPEPASPPASGRADLGSTATQGADR
                     SSPRSDPAVERAVAQDGSDNTTPWQVLETPNFRIFHRNARLAESAGQAAESVRAVQAK
                     RWKSPASQRPWTPPCELYLYPTGKVFAQETQQPETSPGFSTMMCNGNQVVARRTNLRA
                     DHPQVLTAILPHEVTHVVLADLFTVQQIPRWADEGMAVLAEPRAEQQVRAAELQEPLQ
                     AGRIFDLSKLMAMDYPATKDWNLYYAQSVSLTRFLVEQGPPEQFVQFVRDSQRDGIDG
                     ALRSVYRIGGFPELQERWIEYARRQTASLSQARREPKDQGLAAELK"
     gene            complement(1986..>2771)
                     /locus_tag="VHS97_00720"
     CDS             complement(1986..>2771)
                     /locus_tag="VHS97_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX3446742.1"
                     /translation="TQNLFAWLTGVTKTGKENLFFDVILHLGTTAAILVHYRVEIAQA
                     VRGLLGANDAPAGFRRPELIRVGVLAAIATSPLIPHALFFKKMIEKTFEGITFVGFGF
                     LITAAVLLLTAWLKRRDGAKGPAEMTWLDALLIGVAQMFAPLPGVSRSGLTIAAALAL
                     GLSRTWSVGFSLLIAVPAILGAAVWEMRHVDPASLSGNRVVQAVAGTILAGVVGYFAI
                     IWLTRIVRSGRIWYFSVYLVVLGIVVLSLAASVPSYFSSAASP"
BASE COUNT          607 a          944 c          786 g          434 t
ORIGIN      
        1 gaagacttgt tggccgaagc cgaaccgaaa gaccgacccg cgattctgga gctactccgg
       61 aagtcgtacg acgtgatggc gcgcgatgcc aaagcagccg gccgcgatcg ggaagcaact
      121 cactaccgag acaacctcgc gattatcgag cactgccgcg acgcgaacgc cccagccaag
      181 ccacccgacg ccaagcccga caaaccggcc gctcccgggc cactgacgga tcgcgacaag
      241 agcgcgagcg gcacggcggc gccgcagccg cgcccgcatt caccgaaagt tttactccct
      301 ggctcagtgc aagactctgc tccgtcgatt caggctgccg tagcacccgc tctcgagccg
      361 cccatgagcc caattgtcga cgtttcagcg gcgtctccca ggccggtcga cacaccaaat
      421 gcgccccccg cgggcccgac cgccgcatca agctcgccac cgtcgatcaa tattcagccg
      481 gcccgcaacg aggcggatgc gacagttgcc gctccaacca gttcggaacc aacgggctca
      541 gcaggtccgt cctcgactcg cgctgccgcg ggcgcggttc agccggcgaa tactccaaac
      601 gaatctgccg ggccatcact ggcggacggt gatcgactgt tcgcatccgg tcgatacgaa
      661 gacgcggggc gttgctatgc ggcgcttgcc gctcagaacg gtttacccgc ccgtcgccag
      721 gcgcactggg catattgccg catggtgaac gtcgcgcagc ggatcaacgc ccgtccgaaa
      781 acaccccgcg agtgggacga gatcgaggcc gaaatcgtca agatccagcg gttagccccc
      841 aataactggt acggcgagta cctgcgtaac aaggttgccg aagtacgccg aggcgcgcgg
      901 cggacgcagc ccaaatccga caacctggtt gttcggggaa gggcgcccga cgacagtcga
      961 aacaaacccg atccggaacc gcgtcgcttc tcgcgtctct tcggcaaatc gcgcgcccct
     1021 acgcctgcgc cagggcaacc ctcaggcgcg cttgcggcga acaatgcgga gatgccgctc
     1081 aatttgccac aagaatcaca gcctccagag cccgcctcac ctccggccag tggacgcgcc
     1141 gaccttggtt caactgctac acaaggcgcc gaccgatcat cgccccgttc cgacccggcc
     1201 gttgagcgcg ccgtagctca ggatggctca gataacacaa ctccatggca agttctcgaa
     1261 acacccaatt tccgcatctt tcatcgcaat gcgcgcctgg ccgagtcggc cgggcaagcc
     1321 gccgaatcgg ttcgcgcagt tcaggcgaaa cgttggaaaa gtcctgcctc gcagcgaccc
     1381 tggacacccc cttgcgaact ctacctttac cccacgggca aagtctttgc gcaggagacc
     1441 caacaaccag aaacctcacc cggattttcg accatgatgt gcaacggcaa tcaagtcgtc
     1501 gcgcggcgca ccaacttgag ggccgaccat ccgcaggtcc tgaccgccat tctgccgcat
     1561 gaggtgaccc acgtcgtctt ggcagatctt ttcacggtgc agcagattcc tcgctgggcc
     1621 gacgaaggga tggccgtctt ggccgagcca cgggcagagc aacaagtccg cgccgccgag
     1681 ctgcaagaac ctctgcaggc cggccgaatc ttcgatctca gcaagctgat ggccatggac
     1741 tatcccgcca ccaaggactg gaacctttac tacgcccaga gcgtatctct gacccgcttc
     1801 ctcgttgaac aggggcctcc cgaacagttc gtccagttcg tccgcgattc gcagcgtgac
     1861 ggtatcgacg gcgcgctccg aagcgtttat cgaatcggag gattccccga gcttcaagaa
     1921 cgatggatcg aatatgcgcg acggcagacg gcgtcgctca gtcaggcgcg acgcgagccc
     1981 aaggatcaag ggctggccgc cgagctgaaa taactcggaa cagaggcggc caacgagagg
     2041 accacgatcc ccaagacgac caggtaaacc gagaagtacc agatccggcc ggatcgcacg
     2101 atccgagtga gccagatgat cgcgaaataa ccgaccacgc ctgctagaat cgtgccggcc
     2161 acggcctgga ctacgcggtt tccggacagg ctcgccgggt cgacgtgcct catctcccag
     2221 acagccgctc ccagaatcgc cggcacggcg atcagcaagc tgaagccgac ggaccaggtc
     2281 cgcgacaatc cgagcgcgag cgcagccgcg atcgtgagcc cgctgcggct gacccccggc
     2341 aacggggcaa acatctgggc cactccgatc aagagtgcgt ccaaccacgt catctccgca
     2401 gggcccttcg ccccatcacg gcgcttcagc catgccgtga gcagcaagac cgcggctgtg
     2461 atcaagaatc caaatccgac aaacgtgatt ccctcaaacg tcttttcaat cattttcttg
     2521 aaaaagagtg cgtgcggaat caatggcgaa gtcgctatgg cggccaatac gcctactcgt
     2581 atgagctcgg ggcgacgaaa cccggccggg gcatcgtttg cacctaagag accacgcacc
     2641 gcctgggcga tttccactcg ataatgcacg aggatcgcgg ccgtcgtccc cagatggaga
     2701 atcacgtcga aaaagaggtt ttccttgccg gtcttggtga cacccgtcaa ccaggcgaac
     2761 aaattctgag t
//
LOCUS       DATAQL010000045         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2904866, whole genome shotgun sequence.
ACCESSION   DATAQL010000045 DATAQL010000000
VERSION     DATAQL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2904866"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VHS97_00725"
     CDS             complement(<1..1023)
                     /locus_tag="VHS97_00725"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3446743.1"
                     /translation="MNDLGLTVAWLAMQIAILLAPALALNALASRRGPAAGAWVAVLS
                     LGLVVALNVAAFVPRIEWNDKDPGPDIAPPTNEFKNNSAPNFVPNSLRSDVIQPTARS
                     GRTLEWLALAWDRLGRGAAEPAVQVRPWARTLAAVALAGTAAGLLRLTIGLCAVALCC
                     RRGRPVDDPGIIGLLEELRCAMACRRPVALREVPDLTTPATAGRRRPVLLLPDNWRSW
                     SAAERRVVLAHELAHIVRGDYTTGLLARFAVMLNYYHPLVRLMAGRLQLQQEQAADAM
                     GAQFAGGRARYLVALSSLALRQDRRCPRWPARAFLPARGTLIRRIAMLRYESNSKPFD
                     QPLSSAR"
     gene            complement(1020..1403)
                     /locus_tag="VHS97_00730"
     CDS             complement(1020..1403)
                     /locus_tag="VHS97_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3446744.1"
                     /translation="MGRPPTKDLTERELEVMHVFWSCGELTAALARDQLAATGLDRSY
                     TTIANLVRGLNDKGFLRQLNAERPFVYRAVRSYEDVSGRLLGDLVQRVFRGSRAQLLC
                     RLVDERKLTAEERAVLEQIMKEEGR"
     gene            complement(1680..2651)
                     /locus_tag="VHS97_00735"
     CDS             complement(1680..2651)
                     /locus_tag="VHS97_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3446745.1"
                     /translation="MVPIHGICRLAVLGLGTMGHGIAQTFALAGYDVTCFDESASVRN
                     SLIERVRENLAAFVAAELVGPEQVEPTLAHLHLADSEAEAVADCQFLTEAIPEDLAAK
                     QAVLARLERIMAPETILASNSSSFPISQSGRLLEHPERALVTHWFNPPHLTPVVEVVP
                     GPRTSAEIVQATMQLLTHIGKMPIHVRRELPGFLVNRVQVAIQREVWDLVEQGVATRE
                     EIDAAIRGTVGFRFAVMGPLEIHDFGGLDIQLATYRNLVGEIRSDTIAPAVLDGLVAE
                     GHLGIKTGRGFYDYSHERLVARRTRRDSLLLKLWKLLYRNGSSNSMS"
     gene            complement(2694..>2876)
                     /locus_tag="VHS97_00740"
     CDS             complement(2694..>2876)
                     /locus_tag="VHS97_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3446746.1"
                     /translation="RDVSFATVAETMGAKGIHVRKPCEIAGALEQAFAAQGPCVVDVA
                     SDIEAIAPWGDVGASS"
BASE COUNT          546 a          940 c          873 g          517 t
ORIGIN      
        1 ccgcgcactg gacaacggct ggtcgaacgg ctttgaattg gattcatacc gtaacatcgc
       61 gatcctcctg atcagggtcc cgcgcgccgg gaggaacgcc ctcgccggcc agcgtgggca
      121 ccgtcgatct tgtctcagag ccaggctcga taacgccacc aggtagcgag ccctacctcc
      181 cgcaaactga gcacccatcg cgtcggccgc ctgctcctgc tggagctgga gccggccggc
      241 catcaagcgc accaagggat ggtagtaatt cagcatgacg gcgaaccgag ccagcagtcc
      301 cgtcgtgtag tcgccgcgga cgatatgagc cagctcgtgc gcgagcacta cgcgacgctc
      361 ggcggctgac cagcttcgcc agttgtccgg gagcagcagc accggccggc gtcggcccgc
      421 cgtggcgggt gtagtcaggt cgggcacctc gcgcagcgca acgggccggc gacacgccat
      481 cgcgcatcgc agttcctcga gcaggccgat aatccccggg tcatcgaccg gacgcccgcg
      541 tcggcagcag agggcgaccg cgcaaagacc aatcgtcagt cggagcaggc cggcggccgt
      601 cccggccaaa gcgacggcgg ccaacgtgcg cgcccatggc cggacctgta ccgccggctc
      661 ggccgccccg cgcccgagtc gatcccaggc gagcgctagc cactcgagcg ttcggccact
      721 tctggcagtc ggctgaatca catcggacct aagagaattg ggcacgaaat ttggtgcgga
      781 gttgttctta aactcattcg tgggtggcgc gatgtcgggc ccaggatctt tgtcattcca
      841 ctcgatccgg ggcacgaacg ccgcgacgtt caaagccacg accagtccga ggctcaggac
      901 cgcgacccag gcacctgcgg ccggcccgcg gcgcgatgcc aacgcgttca gcgccagtgc
      961 cggcgcgagg aggatggcta tctgcatggc gagccatgcc accgtcaatc cgaggtcgtt
     1021 catcggcctt cctccttcat aatctgttcg aggacggccc gctcctccgc cgttagcttg
     1081 cgctcgtcga ccagccggca cagtagttgc gcccgagaac cgcggaagac ccgctggacc
     1141 aggtcgccca agagccgtcc cgagacgtcc tcgtaagacc gcacagcccg gtacacgaac
     1201 ggccgttcgg cattgagctg gcgcaagaaa cccttgtcat tcagcccccg caccaggttt
     1261 gcaatcgtgg tgtagctgcg atcgaggcca gtcgctgcaa gctggtcgcg ggctagagcg
     1321 gccgtcaatt cgccgcacga ccagaagacg tgcatcacct ccaactcccg ttccgtcagg
     1381 tccttcgtcg gcggccgtcc catggttggc cctcaagtgg tttctagtat ttggtttgtc
     1441 aaatttggtt cggcaaattt accgagccgg cggatgctgt caaccccggt acagtgacac
     1501 gggcatattg cgcgtgtggt tcgtgacgtc acccgccgcg ccctagcgcg gctgcggtcc
     1561 tcgcccgaat tcgctgatga gctggagagg atcctcacgc ggtgtcgtcg aaaaaccaaa
     1621 aacaaagtct gacaccttca acgaaccttt cgataacata ccgccaacta ggctatatgt
     1681 tatgacatcg aatttgaaga accgttgcga tagagcagtt tccagagctt gagcaagagc
     1741 gagtcgcgac gggtacgtcg agcgacgagc cgctcgtgtg agtagtcgta aaaaccccgt
     1801 cctgtcttga ttcccaaatg gccctcggcc acgagcccgt ccaggacggc cggcgctatt
     1861 gtatcgctac gaatctcgcc gacgagattg cgataggtcg caagctggat atcgagcccc
     1921 ccgaagtcgt gaatctcgag cggtcccatg acggcaaaac gaaagccgac cgtgccgcgg
     1981 atggccgcgt cgatctcttc ccgcgtggcc accccttgtt cgacgagatc ccatacctcg
     2041 cgctggatcg cgacctgaac ccggttgacg aggaagccgg gcaattcgcg ccgcacgtga
     2101 atcggcattt tgccgatgtg agtgagtaac tgcatcgtgg cctgaacaat ctctgcgctc
     2161 gtgcgcggac cggggacgac ctcaacgacc ggcgtcaaat gaggcggatt gaaccagtga
     2221 gtcaccagcg ctcgctccgg atgttccagc agccggcccg attgcgagat cggaaaactc
     2281 gacgagttgc tcgccaggat cgtctccggg gccatgatcc gttccaggcg cgccagcact
     2341 gcctgcttgg cggccagatc ttctggaatc gcttcggtca ggaactggca gtctgcgacc
     2401 gcttctgctt ctgagtcggc caggtggaga tgagccagcg tgggttcgac ctgctcgggt
     2461 ccgaccaatt cagcggccac gaaggccgcc agattctcgc ggacacgctc tatcagtgag
     2521 ttccggacgc tcgccgactc gtcgaaacaa gtgacgtcgt agccggccag ggcgaacgtt
     2581 tgagcgatgc cgtggcccat cgtccctagc cccaagacgg cgagtcggca gatgccgtgg
     2641 attggtacca tgttcggggt tgcccctcca acttcatggg atcatgatca cgttcacgac
     2701 gacgcgccca cgtcgcccca tggagcgatc gcttcaatgt cgcttgccac atcaacgacg
     2761 cagggcccct gggccgcgaa tgcctgctct agtgcacctg cgatttcgca aggcttgcgg
     2821 acatggatac ctttggctcc catcgtttcg gccacggtgg cgaagctgac atcccg
//
LOCUS       DATAQL010000046         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3668819, whole genome shotgun sequence.
ACCESSION   DATAQL010000046 DATAQL010000000
VERSION     DATAQL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668819"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1647)
                     /locus_tag="VHS97_00745"
     CDS             complement(181..1647)
                     /locus_tag="VHS97_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446747.1"
                     /translation="MNSVPNFYKKLIYGLVIVFIFGAMIPYTSWLNEEKKTYDLGEAA
                     IGQIDTGSFMMKLLLLGGFRGIFADLLWLRAEEYKRDHDWDRLGTTVDLITKLQPHFL
                     SIWTFQGWNLAYNVAVEWDAPEDKYTWIKQGIKFVQEGVRKNRKSPDLVWDTAWFYFH
                     KMGFSDESIILRRLFRDDEDEAFKTYNDPATHQPKVGNDNFQLGYGWFTQSVELVDSG
                     VTRQSSGTGEEIRYVDPTPQRKGRSDDIAFRSMPAHAQTRYAAGLEKMSIAGIPATFG
                     EVAKAEWYKSWEEWNKLGHFVFMSHNEVPRDGKLVRDKILLDDACYPDRLKQMPDNQA
                     YWTQRWADQMNYRYWKERCAAEQTTNGVLARQLFYEATLAYKTGDFPKAADKFKEGLG
                     VWKTVMNDFPTYRDDDLNKKDTGHWVKRYVAALKQLQVPVPADLPFKDCLALAENDTT
                     VDPFDAIEMIGVVGESKPQTPAPAPMQGPAIAEPKAGK"
     gene            complement(1644..>4058)
                     /locus_tag="VHS97_00750"
     CDS             complement(1644..>4058)
                     /locus_tag="VHS97_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446748.1"
                     /translation="TPELLVRLRWLFLLSDPAVTSNNLAGGLLTWLKAISLICLVCWV
                     ASWLLTGVKHGLIGRGRWFDYIGMVALLLTPVTVLVRVLESVRRLPIYSVAGLPVSTS
                     LVLLFTILYLIWAEIGIARTIRRLGSAFDSFVLIGIHIALAIGMAVGFYVQQKGYLPL
                     VMAMVGPQAQFTWREGLIYGARIGLTYMGYIVFVRVAVLLFTEVIAVRGRRLYSIALL
                     SIYESNRRMWAPWVVTAVFLLVLAFTHWFLQPPRAAEMGRLYVGTLSLLCSVLLTVMV
                     TILTPLSLPTDIQQQTIYTVVSKPVRRLELIWGRLIGYMALVTILVAVFGAISLAYLW
                     RTVHSTIYATEQLAIKAQKENRMTDFKQLKEQADQLRTRMQARVPIKGSLSFLDSRGT
                     PHAVGIDVGQEQGMREPRSHIEGATPSTAIWSFGVVPDPFTPPNRTPVLINRRIPVES
                     FINPGTIEWQLDRVYDLTAQIETVKRSKDQPNLAASKVAEADAAIARNQKELDRARAD
                     YDALTKRAEGLRAEAAQAEAAGNAEEANRLKSEIAKLSSKPITVEMSFNVYRTTKGKV
                     GEPVYAEIQAINPRTGREFDGDVFPIKEYYTNQVQLPAWILAGSAGALRIEIRCLSPT
                     QYLGMSESDLYLLATSGDFGTNYMKGLFGVWLQAMVLTAIGICAGTFLSWPVALLTTI
                     AFFVAGQLAFNFLFDFSRQAILGGGPFESLIRILTHDNQMSDLTPTAGVVLAKTLDSL
                     VMPVMSMLVYLVPNFQALDVTNMVADGFMVNWRTMLINTLLALAYVLPFSFAGFLILK
                     NREVAA"
BASE COUNT          861 a         1187 c         1197 g          813 t
ORIGIN      
        1 gggaaaggta actttgcagt cttggctgga gcccctcgca tcaccaaggg gggaagttac
       61 gttccagctt tgacctggag cccccccaca tcacctgggg ggcaaaggtt accttccagc
      121 cgtcgagctg gaaagaccga ccttcaatgt gcagcaactc gcatcaccag gggtcaaaag
      181 ttactttcca gctttgggct ccgcgatcgc gggaccctgc attggcgcag gggccggcgt
      241 ctgcggcttg ctctcaccta caacgccgat catttcgatc gcgtcgaacg ggtcgacagt
      301 cgtgtcattc tcggcgaggg ccaggcagtc cttgaacggc aggtcagcgg gtaccggcac
      361 ttggagctgc ttgagtgctg cgacatagcg cttcacccag tggccggtat ctttcttgtt
      421 caaatcgtcg tcgcgataag tcggaaaatc gttcatgacg gtcttccaga ccccgagccc
      481 ttctttgaat ttatcggcgg ccttggggaa atcacccgtc ttgtaggcca gcgttgcttc
      541 gtagaagagc tgtcgcgcca gcacgccatt ggtcgtctgt tctgcggcgc agcgctcttt
      601 ccaatagcgg taattcatct ggtcagccca gcgctgcgtc cagtaggcct ggttatccgg
      661 catctgcttc aggcgatcgg gatagcaggc atcgtcgagc aggatcttgt ctcgcaccag
      721 cttgccgtcg cgcgggacct cattgtgcga catgaagaca aaatggccca gcttgttcca
      781 ctcctcccac gatttgtacc attcggcctt ggcgacctcg ccgaaagtcg cgggaattcc
      841 cgcgatgctc atcttttcca ggcccgcggc gtagcgggtc tgggcgtgag cgggcatcga
      901 ccggaaggcg atgtcgtcgg aacgtccttt ccgttgaggc gtggggtcga cgtaacggat
      961 ttcttcccca gtcccagagc tttgacgggt caccccgctg tcgaccagtt cgacggactg
     1021 tgtaaaccag ccatatccca actggaagtt gtcgttcccc actttcggtt ggtgggtcgc
     1081 ggggtcgtta taggtcttga acgcctcatc ctcgtcgtcc cgaaacaagc ggcgcaggat
     1141 gatcgattcg tcggagaagc ccatcttgtg aaaataaaac cacgcggtat cccagaccag
     1201 gtcgggtgac ttcctgttct tcctgacccc ttcctgaaca aacttgattc cctgtttaat
     1261 ccacgtgtac ttatcttccg gagcgtccca ctctaccgcc acgttgtagg cgaggttcca
     1321 gccctgaaag gtccagatgg acagaaagtg cggttgcaac ttggtgatca aatcgaccgt
     1381 cgttcccagg cggtcccagt cgtggtcgcg cttgtattcc tcggcgcgaa gccagagcag
     1441 atcggcgaag atgccgcgaa atccgcccag gagtagcagc ttcatcatga agctgccggt
     1501 gtcgatttga ccgatcgcgg cctcgccgag atcataggtt ttcttctctt cgttcaacca
     1561 gctggtgtac ggaatcatgg cgccgaagat aaagacgatc accaagccgt agatcagctt
     1621 cttgtagaaa ttaggtacgg aattcatgcg gcgacctcgc ggttcttcaa gatgagaaag
     1681 ccggcgaaag aaaacggtaa gacgtatgcc agagccagca gggtattgat tagcattgta
     1741 cgccagttga ccatgaagcc gtcggcgacc atgttggtaa cgtcgagtgc ctggaagtta
     1801 gggacgaggt aaacaagcat cgacatcacg ggcatgacca gcgaatcgag cgtcttggcc
     1861 agcacgacgc ctgcggtcgg agtcaggtcc gacatttgat tgtcgtgggt gaggatccgg
     1921 atcaacgact cgaacgggcc tcctcccagg atggcctgtc gcgagaagtc aaaaaggaaa
     1981 ttgaaagcga gctggcccgc gacaaagaac gcgatggtcg tcaagagggc cacgggccag
     2041 ctcagaaacg tgccggcgca gatgccgatg gcagtcaata ccatggcctg cagccagacg
     2101 ccgaaaagcc ccttcatata gttcgtgccg aaatcccccg aagtggccaa gagatagagg
     2161 tcgctctccg acattcccag atactgagtg ggactcaggc agcgaatctc gatccggaga
     2221 gcgccggctg aaccggccag aatccacgcc gggagctgca cctggttggt gtaatactcc
     2281 ttgatcggga agacgtctcc gtcaaactcc cggccggtgc gcgggttgat cgcctggatt
     2341 tccgcgtaaa cgggctcgcc aaccttgcct ttggtcgtcc ggtagacgtt gaagctcatc
     2401 tcgaccgtga tcggttttga cgacagcttt gcgatctcgc tttttaaccg attggcctcc
     2461 tctgcgttac cggcggcctc cgcctgggcg gcttccgctc ggagtccctc ggcccgtttc
     2521 gtcaaggcgt cgtagtcggc gcgggctcga tccagctctt tctggttgcg tgcaatggcc
     2581 gcgtcggcct cggcgacctt ggaagctgcc aggttcggct ggtccttcga gcgtttgacg
     2641 gtttcgatct gagcggtgag atcgtaaacg cggtcgagct gccactcgat cgtgccaggg
     2701 ttgataaagc tctcgacggg gatgcgacga ttgatcaaga ccggagtacg gttcgggggc
     2761 gtgaacggat cggggacaac gccgaagctc cagatggccg tcgagggggt cgccccttcg
     2821 atgtggctgc gcggttcacg catcccctgt tcctgaccca cgtcaatgcc cactgcgtgc
     2881 ggcgtcccgc gcgaatcgag gaacgacagc gatcctttga tgggaactcg tgcctgcatg
     2941 cgcgtgcgga gttgatcggc ctgctctttc aactgcttga aatcggtcat ccggttttct
     3001 ttttgggcct tgatcgccag ttgctcggtc gcatagatcg tcgaatggac cgttcgccag
     3061 aggtaagcca ggctgatagc gccgaaaacg gccaccagaa tcgtcacgag cgccatgtag
     3121 ccgatcagcc gaccccagat cagctcgagc cggcgcaccg gcttggagac aaccgtgtaa
     3181 attgtctgct gctggatatc cgtgggcagc gaaagcggcg tcaagatcgt caccatgacg
     3241 gtcaagagca ccgagcagag caaactgaga gtgcccacgt aaagtcggcc catctccgcg
     3301 gcccgtggag gctggagaaa ccagtgtgtg aatgccagca caaggaggaa caccgcggtg
     3361 accacccacg gagcccacat gcgccggttg gactcgtaaa tgctgaggag agcgatcgag
     3421 taaagacggc gcccccgcac ggcgatcact tccgtgaaga gcagcacggc gacccgcacg
     3481 aaaacgatat agcccatgta cgtcaggccg atgcgcgcac catagatgag cccctcgcgc
     3541 catgtgaact gagcctgcgg gcccaccatg gccatgacca gaggcagata acccttctgc
     3601 tggacataaa agccgactgc catgccgatc gctagagcga tatggattcc gatcaaaaca
     3661 aaggaatcaa aagcactccc cagacggcgg atggtccgcg cgatgccgat ctccgcccaa
     3721 atcaggtaaa ggatggtgaa cagaagaacg agcgacgtgc ttaccgggag cccggccacg
     3781 gaataaatcg gcagtcgcct caccgactcg aggacccgga ccaggacggt gaccggcgtc
     3841 aacaagaggg caaccatgcc gatatagtca aaccaacggc cgcggccgat cagtccgtgt
     3901 ttcacccctg tcagcaacca cgatgcgacc cagcacacca gacagatcag gctgatggct
     3961 ttgagccaag tgagcagacc tcccgccaga ttgttgctgg taacggctgg gtcgctcaag
     4021 agaaagagcc acctcaaccg aaccagcagt tcgggcgt
//
LOCUS       DATAQL010000047         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1989533, whole genome shotgun sequence.
ACCESSION   DATAQL010000047 DATAQL010000000
VERSION     DATAQL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989533"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VHS97_00755"
     CDS             complement(<1..1050)
                     /locus_tag="VHS97_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3446749.1"
                     /translation="MFEDLLRRLGIGETNSGAYCGDGIECGGNVEIHSTNPATGRPLP
                     SLRSASVADYERVVTQASQVFQSWRMIPPPRRGEVVRQIGVALRARKADLGLLVTLET
                     GKIRTEGEGEVQEMIDMCDFAVGLSRQLYGLTIASERPRHQMIEQWQPLGPIGIITAF
                     NFPVAVWAWNAMIAAVCGDTMIWKPSPLAPLTAIAVQGIVNDVARVNGCEGVFNLCMG
                     GADDVGERMLHDPRLPLISASGSCRMGRRVGEVVGRRLGRALLELGGNNAIIITPSAE
                     LELAIRATAFAAVGTAGQRCTSVRRLIVHESIHDAVLNRLIQVYSRVKIGDPWDESVL
                     LGPLINERAVETMMAA"
     gene            1225..>2573
                     /locus_tag="VHS97_00760"
     CDS             1225..>2573
                     /locus_tag="VHS97_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3446750.1"
                     /translation="MKLFYRPKRSSGARQRLFDFLDRSRRRQRAFKLAILGLTSLAIA
                     LVLGLLPKGRYVVAAAHSTVRQAARGVVGLRTPREEIDENWRRFRLDGIAESKKALVQ
                     VFAKASAGNQRLMRYAGLDPDHGLLRWGNYDRTLLLPSTVFEADNNGRSYRFRACTDS
                     IWLRQITIQSGVLMFFQVMDGPQLAEAIRGTEAIPVLESRQSTNSWGLRGPEPELDAP
                     LRGIVLGDSFMQGMFIGDDETPPECLRHDLESRLKIKVSIVNTGHIGYSPEQYFYSLV
                     EYADRFRPHFVVVSVFGNDFAGEIADVVTSGKGDWDEGKYWLDKIITLCMARGWTYVI
                     VPVPYEPVLLGRRKAGHFPGTISTILATNAFNFLDPSDGLLDDFLELAVEGERVGKRP
                     YGCPLFNVHIGDGHFSALGSRSWARIVGRRLELLLERTQSLDNVIRSSEAPPRDDASL
                     "
BASE COUNT          489 a          803 c          769 g          512 t
ORIGIN      
        1 ggccgccatc atcgtctcga cggcccgctc gttgatcagc ggccccaaca gcaccgactc
       61 atcccaggga tcgccaatct tgacccgtga gtaaacctgg atcagccggt tcaggacggc
      121 gtcgtggatc gactcatgaa ctatcaggcg gcggacgctc gtgcagcgct ggccggccgt
      181 gccgacggcg gcgaacgcag tcgcccggat cgccaactcc agctcggcac tcggtgtgat
      241 gatgatggca ttgttgcccc ccaactcgag gagggcccgt cccaaccggc ggccgacgac
      301 ctcgccgacg cggcgaccca tccgacagct cccgctcgcc gaaatcaaag gtagccgcgg
      361 gtcgtgcagc atccgttcgc cgacgtcgtc agccccgccc atgcagaggt taaagacccc
      421 ttcacacccg ttgactcggg cgacgtcgtt gacaattcct tgcacggcga tcgcggttaa
      481 cggcgccagc ggcgatggct tccagatcat ggtgtcgcca cagaccgcgg cgatcattgc
      541 attccatgcc cacacggcga ccgggaaatt gaacgcggtg atgatcccaa ttggccccag
      601 cggctgccac tgctcgatca tctggtggcg cggccgttcg ctggcaatgg tgaggccgta
      661 aagttggcgc gacagtccga cggcgaagtc gcacatgtcg atcatttcct ggacctctcc
      721 ttccccctcg gtccggatct tgccggtctc gagcgtgacc aggagaccca gatccgcctt
      781 tctggcgcgg agcgcaacgc cgatctgccg caccacctcg ccgcgccgtg gtggagggat
      841 catgcgccag ctctgaaata cctggcttgc ctgcgtgacg acacgctcgt aatctgccac
      901 agacgccgac cgcaacgagg gcagaggccg gccggtcgcg ggatttgtgg aatggatctc
      961 gacgttgccg ccgcattcga tgccgtcgcc acaatacgcg ccgctgttgg tctcaccgat
     1021 accgagccta cgcaagaggt cttcaaacat ggcgtcgtgc ctttccgcct cgaaatgagg
     1081 atgaattcgc ccctatttcc catcataccg ccggagccgg gggaaggatg cgttctctgg
     1141 cgtgaaaaca ccggttgatc ctgtcgggct cgtcaacgaa aatggatcag tgtctccaac
     1201 gacagcgagc gagtcgaggc gtcgatgaag cttttttaca gaccgaagcg atccagcggc
     1261 gctcgacagc ggctatttga cttcctcgat cggtcacgcc gtcgccaacg tgcgttcaaa
     1321 ctggcaatcc tgggtttgac gtccctggcg atcgcgcttg ttctcggctt actgcccaaa
     1381 ggacgctacg tcgtggcggc agcgcactcg accgtgcgtc aggcggcgcg aggcgtcgtg
     1441 gggcttcgca cgccgcgcga agagatcgac gaaaactggc gccggttccg gctggacggg
     1501 atcgccgaat ccaagaaagc tttggtgcaa gtcttcgcga aagccagcgc cggtaatcaa
     1561 cgcttgatgc gctatgccgg gctcgatccc gatcatggcc tgcttcgttg gggaaactac
     1621 gaccgcaccc tcctgctgcc gtcgacagtc tttgaagctg acaacaatgg tcgttcctac
     1681 cgatttcggg cctgcaccga ctcgatctgg ctgcgacaga ttacgatcca gagcggggtc
     1741 ttgatgttct tccaggtcat ggatggacct caactggccg aggcgatccg aggtaccgag
     1801 gcgatccctg tcttagagtc acgtcagtcg accaattcgt ggggtctgcg cggtcccgag
     1861 ccagagctcg acgcgcccct gcgcggtatt gttcttggtg attcgttcat gcagggaatg
     1921 ttcataggcg acgacgaaac gcctccggaa tgcctgcggc atgacctcga aagccgcctg
     1981 aagatcaaag tctcgatcgt caacacggga catatcggtt actcgcccga gcagtacttt
     2041 tattcgctcg tcgagtacgc cgatcgcttc cgcccgcact tcgtggtcgt gagcgtcttc
     2101 ggcaacgatt tcgcaggcga gattgctgat gtcgtcacgt caggaaaagg agattgggac
     2161 gagggaaagt actggctcga taaaatcata actctgtgca tggcgcgcgg ctggacgtac
     2221 gtaattgtgc ccgtccctta cgagcccgtt ttgctgggcc gccgcaaggc cgggcatttt
     2281 cccggaacga tttccacgat ccttgcaacc aacgccttca attttctcga cccgagcgac
     2341 ggtctactgg acgattttct tgagctagcc gtcgaaggag aacgagtcgg caagaggccg
     2401 tacggttgcc ctctgttcaa cgtccacatc ggcgatggtc acttctctgc gctgggatcg
     2461 cggtcctggg cgagaattgt ggggaggcgg ctggagctct tgctggaaag aacgcaatcg
     2521 ctcgacaacg tgattcgctc gagtgaagcc ccgcctcgcg acgatgcaag cct
//
LOCUS       DATAQL010000048         7395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1531622, whole genome shotgun sequence.
ACCESSION   DATAQL010000048 DATAQL010000000
VERSION     DATAQL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531622"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..717)
                     /locus_tag="VHS97_00765"
     CDS             complement(208..717)
                     /locus_tag="VHS97_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446751.1"
                     /translation="MMKGNFTGKGRGKQDADRAHGGGRESAAVPVPIDDLILRDYLTD
                     SLAPEESARVEKALRDSAELRAQLEEVRQNRADGQLHTLGAIWNRSRLTCPSRQQLGS
                     FLLDALDPELASYLTFHLEVIECPFCQANLADLKAQSQTATAAHASQTRQRRILQSSQ
                     HLLSEDKKS"
     gene            complement(721..1350)
                     /locus_tag="VHS97_00770"
     CDS             complement(721..1350)
                     /locus_tag="VHS97_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446752.1"
                     /translation="MAVTETDSLLVRHVRAGNAEAWRQLIERFEGRLLAFVDSRLRDR
                     AASEDVVQETFIGFLTSLPHYDEKRDMEAYLFSIAAHKLTDHLRKQGRRPIDQFGSDD
                     HGRPLDEVPGGGRAASSIARSGERKKAEERHLTEAMGQLVREWLARADYNRLRCMELL
                     VVKGWANKDVAEHLGITEQAVASYKFQTIARLKEMARRAGLSLEELGGG"
     gene            complement(1540..2022)
                     /locus_tag="VHS97_00775"
     CDS             complement(1540..2022)
                     /locus_tag="VHS97_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446753.1"
                     /translation="METLAAFIVFAAFVVAFFGSIFGLAQVLSPDRIRRLGQNIWPLR
                     LTLWQMMSGVAVAAFLILAFEKGYETALVLVVVSLFVLAWFVRAWSHEFVFLMGLRDI
                     DLPARHDKLIWAFLLLIFPPITTWLFRSFRLAHWPEPVQETQAPLRSETKGTTATQPA
                     "
     gene            complement(2051..3064)
                     /locus_tag="VHS97_00780"
     CDS             complement(2051..3064)
                     /locus_tag="VHS97_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446754.1"
                     /translation="MKRVLFLIVLVMLVSWLLVARRNAVGAARRRAFESHQRSIRALE
                     AGRPRPVISRRDPRLESRQQTRRALAEAREALAEARNEVHHAFAEARDDVRAAFDEVR
                     VALVSDDDPAHGPCPVPAPKALASEEVDGLPVPIVPGTRVTKAEATPPIHPRPVVRVH
                     APAPPAPPVLPTPAPVAVPAVVKASVPQTVTGQLSANKERAIADARHQLRSRIVHWLD
                     PEVPGSWSPPTRLIDAMILGQPQIRRIQKDYGELFEATLTVDTSPVRRNALIEVYNRQ
                     LVERRMASLGGALAFVLICLAAVSGYVRADEATKGYYTNRLRLLSAAGVGAAGVIIYH
                     MVV"
     gene            complement(3194..5155)
                     /locus_tag="VHS97_00785"
     CDS             complement(3194..5155)
                     /locus_tag="VHS97_00785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3446755.1"
                     /translation="MTATAEDLPNVDLNDHNRMGETVAYEPKTSSNSGPLAPSGSSDM
                     PKANYTPQVHGRYTFGSGARPLDGYTIKRAIGRGGFGEVYYATSDSGKEVALKLILRN
                     LDVERRGVVQCMNLKCPNLLTIFDLKSNDADDTFVIMEYVAGPSLASVLKQYPAGLPE
                     AEVRNWLKGLVEGVAYLHDHGIVHRDLKPANLFMEEGIVKIGDYGLAKLITPSQGTEH
                     SESIGTCHYMAPEIGSGKYHKPIDIYAMGVILYEMLTGRVPFEGETVNEVLMKHLTAR
                     PDVSVLPKPYQAIVAKALAKDPNHRVAKVYDLLAPGDAPRTPDVRIIGDGKAGPGAGT
                     RLADGTAVRAAQEDDILRIEAEEPIFYIGPDTRPPGAAGRNAIAQRLRANWAALRRPA
                     RYQSPPPSPGRNRPVTAQASPVHGGAVRQAAYASPASKQPIRKVAAPPPEPPPLGSGR
                     VRTAELAASMLWSAPLVALLIIPAATILGIDLSNDPQQLAVLYGMALLGTWTTLVPNK
                     LFEARKIDGTNRRLLAMAAGLLLGGVSLALGRLLQLDFAPQTAFFDNSRNLGPVYFGA
                     LYAVMSGWSSLVARDRVTRFRILPIVGTGLVAAALIPFWPHTRQDGIAVAVLIATAVQ
                     LVSPWNEAASLYARYVRACEKQRRTGQVV"
     gene            complement(5284..6960)
                     /locus_tag="VHS97_00790"
     CDS             complement(5284..6960)
                     /locus_tag="VHS97_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3446756.1"
                     /translation="MTNHSSWLPAAQEITIPMVNPGGESTMLGHWRIVLRQAEEAARA
                     GRFADAYALASRPDVADHHHAVQFRSRLALDLIARATRRGAVDDLAGAVEDLDLAERI
                     GAPPDSLAAARLNLADRVAEDVRVDLDAGDPVRVLERIEELARHKIGGPSLRRAREIA
                     DAWQSALAEGRRGEFGRAQEQLDRAERLAAGAGAIAAQQAVAAAKTDLETRQRGAASK
                     VEALYAALSEGKWPQILSAAEAVLVCVPEHPAARQARSRAWQQIAAIGPGAAAQSPQR
                     GARAAQGAAMGEDSNSVRESAEPGGSTEPDGIVWLTAAAKHEGVSPSLGAGGSTPAAG
                     FSPRPSVLPRVPMQPRPLARVDAAGPKGRFLLWVDAVGGYLVCLDDQVVLGRAGPDSH
                     ADVPLMGDLSRNHASLIRNGESYLLRAHHPSFVNGKPVADEAVLRDGDVIRLGSTVEL
                     EFRQPSPVSATARLSILSRHRLPLAVDGVLLMAETCIVSSALQAHIPAPALSNPVVLY
                     RQAGVLWCRAAGAFDVDGRTCASRAPLTLQSSVLGDGFSFSLEPLGTQPV"
     gene            complement(7124..>7395)
                     /locus_tag="VHS97_00795"
     CDS             complement(7124..>7395)
                     /locus_tag="VHS97_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446757.1"
                     /translation="EAKLRVLEAAQTKNESNFDGSTLSQAKKTIADLEKRLNVMSHRA
                     AIDGRYGDLDSAPTYVDPRRDVIKEVEEKFGDSTRPAEKSGDKSL"
BASE COUNT         1425 a         2328 c         2276 g         1366 t
ORIGIN      
        1 gtttgcaccc caggctgcaa catgtcgccc cgttcggggc ttttcggtct cccgcccgtc
       61 aacgaagccg caacaagcgc tccggcctgt caataccaga ttttcaccac gccagcctcg
      121 ccctcccaaa aaaggatcga atccgcccgg gcgaggtatc gcttggtgat tcgaagcgcg
      181 ccagtcaccg cgcacccagc agtgtgctca gctcttcttg tcctcgctta gcagatgctg
      241 gctcgactgc agaatacggc gctggcgtgt ctgggacgca tgggccgcgg tagcggtttg
      301 agactgggct tttaaatcgg cgagattcgc ctggcagaag gggcactcaa tgacctcgag
      361 gtggaaggtc agatacgaag caagttcagg gtcgagcgca tcgagcaaaa aactacccag
      421 ttgctggcgg ctggggcatg tcaagcgcga ccggttccag atggctccga gggtgtggag
      481 ctgcccgtcg gcgcggttct ggcggacttc ctcgagttgg gcgcgaagct cggcggaatc
      541 gcgcaaagcc ttttccaccc gggctgactc ttccggtgcc agcgagtcgg tcaggtagtc
      601 gcgcagaatc aagtcgtcga tggggactgg aacggcggcc gattcgcggc cgcctccgtg
      661 cgcgcgatcg gcgtcttgct tgcctcttcc cttgcctgtg aagttaccct tcatcatcga
      721 tcaacctccg ccaagctcct cgagagaaag ccccgcgcgg cgggccatct ccttgaggcg
      781 ggcgattgtc tgaaacttgt agctggcgac cgcctgctcg gtgatgccga gatgttcggc
      841 gacgtccttg ttagcccatc ccttgacaac gaggagctcc atgcaccgaa gccggttgta
      901 atcggcccgt gcgagccact cgcggaccag ttgccccatt gcctcggtca aatgccgctc
      961 ttcggccttc ttgcgttcac cgctccgcgc gatgctggag gcagcccggc caccgcccgg
     1021 cacctcgtcg agcggccggc cgtggtcgtc ggagccaaat tggtcgatcg gccgccgccc
     1081 ctgcttccgc agatgatccg tcagcttgtg cgccgcgatc gagaacagat acgcttccat
     1141 atcccgcttt tcgtcgtaat gcggcagact cgtcaggaag ccgatgaacg tctcctgaac
     1201 cacatcctcg ctggcggcac gatcgcgcaa ccggctatca acaaacgcca gtaggcgtcc
     1261 ctcaaaccgt tcgatgagct gacgccaggc ctcggcattg ccagcgcgca catgccggac
     1321 gagaagggaa tcggtctctg taacagccat cgattgagtc tgccccggga cggattttcg
     1381 ctcccagacg aactaccatg cccgattgta atcattgccg tgaccgtttg gctatcctct
     1441 ccatccttat ccatcaagac atgtgttgag aagcgatgtc gcgatttgtc caacacaatg
     1501 aggggaagca ccctataggg ccgcggcgcc cgagcacatt caggctggtt gggtcgccgt
     1561 cgttccctta gtttctgacc ggagcggagc ctgagtctct tgcactggct cgggccaatg
     1621 agccaggcga aacgagcgga ataaccaggt agtgatcggc gggaaaatca ggagcaagaa
     1681 agcccagatc agcttgtcat gccgggccgg caggtcgatg tcacgcagtc ccatcaggaa
     1741 gacgaattca tgagaccagg cgcgtacaaa ccacgccaga acaaaaaggg agacaacgac
     1801 gaggacgagc gcagtctcat agcctttctc gaaagcaagt atcaaaaacg ccgccacggc
     1861 caccccgctc atcatttgcc agagagtcag ccgcaaaggc caaatgttct gcccgaggcg
     1921 ccgaattcgg tccggcgaaa gcacctgagc caggccgaat atcgagccga agaacgcgac
     1981 cacaaacgcc gcaaagacga tgaaggctgc caaggtctcc atgggccgaa tccccctatt
     2041 tgccgttgga tcagaccacc atgtggtaga tgatcacgcc agccgcgccg actcccgcgg
     2101 cggacaagag ccgcaggcgg ttcgtgtaat agcccttggt cgcctcatcg gcacgaacgt
     2161 agccggagac ggcggccagg cagatcaaca cgaaagccag ggctccgccc aggcttgcca
     2221 tccgccgctc gaccagttgg cgattgtaga cctcgatcaa tgcgttgcgg cgtacaggtg
     2281 aagtgtcgac tgtgagcgtg gcttcaaaca actctccgta atctttttga atccgcctga
     2341 tctgcggctg ccccaaaatc atcgcatcga tcaatcgggt aggaggcgac caggatccag
     2401 gaacttcggg atcgagccag tggacaatcc tggatcgcaa ctgatggcgc gcatcggcga
     2461 tggctcgctc cttattggct gaaagttggc ccgtcacagt ctgcggcacc gatgccttta
     2521 caacggccgg gactgcgaca ggtgctgggg tcgggagtac gggcggagcc gggggagcag
     2581 gagcatggac tcttacgacg ggtcgcggat gaattggggg cgtggcctcg gccttggtca
     2641 cgcgggttcc cggcacgatc ggcacgggga gaccatcaac ctcttcgctc gccaaagctt
     2701 tgggggcagg gactggacat ggcccatgag ctgggtcgtc gtcactaacc agtgcgacgc
     2761 gcacttcatc aaacgccgct cgcacatcgt cgcgggcctc ggcgaacgcg tggtgaactt
     2821 catttcgagc ctcagcgagc gcttctcggg cctcggcgag tgcacgccga gtttgctgac
     2881 ggctctcgag ccgcgggtct ctacgcgata tcaccggtcg aggtcgaccc gcttcgagtg
     2941 cacgaatact tcgctgatgg ctctcgaatg ctcggcgacg cgcggcccca accgcattcc
     3001 gccgagcaac caagagccac gacaccagca tcacaaggac gatcaagaac aacacgcgtt
     3061 tcattgaagt cgcatcctct gacggccagc gatccgcttg ttttgggcta atggcaagtg
     3121 acgaccaaga cagaattcct gcagacgctc ggcgtttgca cgcctcaatt tccttccaaa
     3181 tctgtgccag aatctacaca acctggccag ttcgtctttg cttttcgcag gcgcgaacgt
     3241 aacgtgcgta gagcgaagca gcctcgttcc aggggctgac cagttgaacg gccgtggcga
     3301 tcaagacggc gacggcaatt ccgtcttgtc gcgtatgcgg ccaaaacgga atcagtgctg
     3361 ccgccaccag acccgttccc acgatcggca ggatgcggaa ccgcgtcacg cggtcgcgcg
     3421 ccaccagcga tgaccatccg ctcatgaccg cgtacaaagc tccgaagtag accgggccca
     3481 ggttgcgtga gttgtcgaaa aacgccgtct gaggcgcgaa gtcgagttgt aacaaccgcc
     3541 ccagcgcgag actaactcct cccagcagta gtccggcggc catggcgaga agtcgccggt
     3601 tagtcccgtc aatcttgcga gcctcgaata gcttgttagg aaccagggtc gtccacgtcc
     3661 ccaagagcgc cataccatag aggacagcca gttgctgtgg gtcgttcgat agatcaatgc
     3721 ccagaatggt ggcggcaggg atgatcaaga gcgccacgag aggggcggac cagagcatcg
     3781 acgctgccaa ttcggcagtg cgaacgcggc cgctgcccaa aggggggggc tcggggggcg
     3841 gggcggcgac cttccggatt ggctgtttgg aagctggact cgcgtacgcg gcctgccgaa
     3901 cagcaccgcc gtgcacaggg gaagcctgag ccgtgaccgg ccgatttcgc ccgggggacg
     3961 gtggcggtga ttggtagcgg gcagggcgcc gaagcgccgc ccagttcgcc cgcaaccttt
     4021 gggcaatcgc gttgcgaccc gcagcgcctg gcgggcgggt gtccggacca atgtaaaaga
     4081 ttggctcctc ggcctcgatc cggagaatgt cgtcctcctg agccgcccga acggcggtcc
     4141 cgtctgccaa cctagtcccc gctcctggcc ccgccttgcc gtcgccgatg attcggacgt
     4201 cgggggtgcg tggcgcgtct ccgggagcaa gtaggtcgta gaccttggcg accctgtgat
     4261 tgggatcctt ggcgagtgcc ttggcgacga tcgcctgata tggcttggga agcaccgata
     4321 catcggggcg agccgtcaag tgcttcatca gcacctcgtt gactgtttcg ccctcaaacg
     4381 gcacccgccc ggtcagcatc tcgtagagga tcacacccat ggcataaata tcgattggct
     4441 tgtggtactt gcccgaaccg atctcaggcg ccatgtaatg gcaagtgccg atactctcgg
     4501 agtgctcggt tccctggctc ggggtgatca gcttggccaa gccgtagtcg ccgatcttga
     4561 cgatcccttc ctccatgaac aaattggccg gcttcaggtc gcggtggacg atcccatggt
     4621 cgtggaggta ggcgacgcct tccactaatc ctttgagcca gttgcggacc tcggcctcgg
     4681 gcagtccagc ggggtattgc ttcaagacgc tcgccaggct gggaccggcg acatattcca
     4741 tgatgacgaa cgtgtcgtcg gcgtcgttgc tcttgaggtc gaagattgtg agtaagttcg
     4801 ggcacttgag attcatgcac tgcacgaccc cgcgccgctc gacatcgaga ttgcgcagga
     4861 tcaatttgag cgcgacttcc ttgcccgaat cggacgtggc gtagtagacc tcgccgaaac
     4921 ccccgcggcc gatggctcgc ttgatggtat agccatcgag cggccgcgct ccggaaccga
     4981 aggtgtagcg gccgtgtacc tgcggggtgt agttggcttt cggcatgtct gagcttcccg
     5041 aaggggctag aggccctgag ttcgacgacg tcttaggctc gtacgcgacc gtttcgccca
     5101 tacggttatg atcgttcaaa tccacgttag gcaggtcttc agccgttgca gtcatgagtt
     5161 gtccaactcc cacagagagg gcctactcca ccggcaaagg ccgcttggct gcgcgacccg
     5221 cgccgatcac ctattgtcgg gcccggccag ctgtgccggc ctcctataat aacaattcgc
     5281 ttcctacaca ggctgagttc ccagaggttc aagactgaac gaaaagccgt cgcccagaac
     5341 gctggattgc aaagtcagag gagcgcgcga agcgcaagtt cggccgtcga catcaaaggc
     5401 gcccgcggca cggcaccaca gcacgccggc ctggcggtag agaacgactg gattactgag
     5461 cgccggggcg ggaatgtggg cctgtagcgc actactgacg atgcacgtct cggccatcaa
     5521 gagtacgccg tcgaccgcca ggggaagacg atgccggctc aggatcgaca accgtgccgt
     5581 ggcactcacc gggctgggct ggcggaattc gagctcgacg gtcgaaccta accggatcac
     5641 gtcaccgtca cgaaggacag cctcgtcagc gaccggctta ccgttgacga acgatggatg
     5701 atgggcccgc agcagataac tttcgccgtt gcggatgaga gatgcatgat ttcgagagag
     5761 gtcgcccatc aggggaacgt ctgcatgact atcgggcccg gcccgaccca agacgacttg
     5821 atcgtccagg cacaccaggt aacctccgac cgcatcgacc cacaggagga agcggccctt
     5881 gggcccggcc gcgtcaacac gtgccaacgg tcgcggctgc atggggacgc gaggtagcac
     5941 cgacggacga ggcgaaaacc cggcggccgg ggtgctcccg ccagcaccaa gagacggaga
     6001 aacgccttcg tgctttgcgg cggctgttaa ccagactatc ccgtcgggct cggtagagcc
     6061 cccaggctcg gccgattcgc ggaccgagtt agaatcctcg cccatggcgg caccctgcgc
     6121 ggctcgggcg ccgcgctggg gcgactgggc agcggcgccg ggtccgatcg ccgcgatctg
     6181 ctgccacgcc cgtgaacgag cttgccgcgc ggccgggtgc tcgggcacac ataccagcac
     6241 ggcctcggcc gcggagagga tttgcggcca tttaccttct gaaagtgccg catagagcgc
     6301 ctcgaccttg gaggcagccc ccctctggcg ggtttccagg tcggtcttgg ccgcggctac
     6361 tgcctgctgg gcggcaatcg cgccggcgcc agcggcaagg cgctcggccc gatcaagctg
     6421 ttcctgcgcc ctgccaaact cgccgcgccg cccttcggcc agcgccgact gccaggcatc
     6481 ggcgatctca cgcgcccggc gcaacgatgg gccgccgatc ttgtgtcgtg cgagctcttc
     6541 gattcgctcg agcactctca ctggatcgcc ggcatccagg tcaacgcgca cgtcttcggc
     6601 gacccggtcc gccaggttaa gtcgggccgc ggccagcgaa tcgggcggtg cgccaatccg
     6661 ttccgccaag tccaggtctt cgacggctcc cgccagatcg tcgaccgctc cgcgccgcgt
     6721 cgcccgagca atcaagtcga gcgccagccg gctgcgaaac tggactgcat gatggtgatc
     6781 cgccacgtcg ggccgactcg cgagagcata agcgtctgcg aaccgacctg cccgtgcggc
     6841 ttcctctgct tgccgcaaga cgatgcgcca atgtcccaac atggtagatt cgccgcccgg
     6901 gttgaccatg gggattgtga tctcctgagc ggccggtagc cagctcgagt ggttggtcaa
     6961 gaaaccctcg ggctcttgac caaccagtcg ccgctgacca tcagccgttt gccactagcc
     7021 accgtcgggt ggctaatggc cagtaaaacc gaattgcatg gtattgtcac tagccactga
     7081 tcgattgcca cacgaggccg tacttgccag gatcgctttt ggatcagagg ctcttgtcac
     7141 ccgacttttc ggccggccgg gtggaatcgc caaacttctc ttcgacttcc ttgatcacgt
     7201 cgcggcgggg atcgacgtag gtcggtgcac tgtcaagatc gccgtagcga ccgtcgatcg
     7261 cggcccgatg cgacatcaca ttaagccgct tctcgaggtc ggcaatcgtc ttcttggctt
     7321 gggatagtgt gctgccatca aagttagact cgttcttggt ctgggcagcc tcgagcacgc
     7381 gcagcttggc ctcga
//
LOCUS       DATAQL010000049         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1836843, whole genome shotgun sequence.
ACCESSION   DATAQL010000049 DATAQL010000000
VERSION     DATAQL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836843"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..>3130)
                     /locus_tag="VHS97_00800"
     CDS             complement(169..>3130)
                     /locus_tag="VHS97_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3446758.1"
                     /translation="AKQKGGLRLDARTALLVGGSTGPALVPGNPAESLLVDAINYGEA
                     LQMPPKSKLPDAEIAALTEWVKRGAPWGIQTPVATANAADAKPSDKSDQLSREEYQKR
                     ARFWSFQPIKQVTPPALKTGPLDWARNPIDQFIQSAMTEHGLAPSPEADRRTLIRRLS
                     FDLLGLPPSPTEVAAFVADAGADAYERLVDRLLASPRYGERWARHWLDLVRYAETGGH
                     EFDYDIVNAFGYRDYVIRALNADLSYDRFVTEQIAGDLLDPPRRSAGAGSNESILGTG
                     FYFLGEGTHSPVDVREEQMRRIDNQIDVMSKAFLGLTVACARCHDHKFDPITNKDYYA
                     LAGVLRSSRHQQAFIDPPQRFAPAVGRLLSLKSTIASLIREAMTQAPEVFSRETATVW
                     TRCASDESSARQTTRPSRSPKSDEDETVFEDFHGDSFDGWSVTGDAFGDGPSRAGGLR
                     LELVNGGSRLVSIKPGQAHSGLISDRLRGVLRSRSFTIENRYVHWLVAGKGGRISVVV
                     DGFEKIRDPIYGELTRRIDAGDHPRWVTQDLGMWIGHSAYFEISDGSTVDFGGGSAQI
                     EEGRGYIAVDEIRLSNRSTPAPPVGSDSDQLPGVTEPALELAGVIKALHATAHDAAAD
                     RLAAAVEEVRATEQQIPDPTLALAIADGTGQDERIHIRGSHKNLGEIVPRRFLEILGG
                     SDSATSAGSGRLYLARRMVDPRLNPLLSRVLVNRLWKHHFGEGIVKSTDDFGAMGQKP
                     SHPELLDWLVSELLAHDWSWKAIHRLMVTSSTYRMVSTPHGDLERLDPTNTFLHRMNV
                     RRLEAEAIRDALLAVSGRLVPSMYGKSTPVHLTSFMEGRGRPRQSGPLDGDGRRSLYL
                     SVRRNFLNPMFLAFDTPAPFSTMGRRNVSNVPAQALTLMNDPLVVGQARLWAERIVAG
                     PSQSAPVRLDNLYEIAFSRPPTLDEARACLEFLDRQMQAGHAGQGYDTQRQALAWTAL
                     CHVLVNMKEFIFID"
BASE COUNT          592 a          953 c          987 g          598 t
ORIGIN      
        1 cggcgatcct tgccccaaag gccggctcac gaaatagcgc cgcaagagcc agcgcgccaa
       61 atcccgcacc gcaccgcaat aaaaggtcgc gccggtccag tccaggttcg acaaagtgtc
      121 ggcaatgcat ggaggttccg cgattgctgt agatggaacg actcgggttc agtcaataaa
      181 aataaactct ttcatgttga caagaacatg gcaaagggcg gtccacgcga gcgcttgcct
      241 ttgagtatcg tacccctgtc cggcgtgccc ggcttgcatt tggcggtcga gaaactccag
      301 gcatgctctg gcctcatcta aagtcggagg tcgggaaaag gcgatctcgt acaggttgtc
      361 tagccgcacg ggggccgatt ggctgggtcc ggccacaatg cgctcggccc agaggcgagc
      421 ctggcctacc accagggggt cgttcatgag cgtgagcgcc tgggccggga cattcgacac
      481 gttgcgccgg cccatggtcg agaacggagc cggcgtgtcg aaagccagga acatcggatt
      541 gaggaaattg cgccgaacgc tcaaataaag gcttctccgg ccgtcgccgt cgagcggacc
      601 ggactgacgg gggcggccgc ggccctccat gaaactggtc aggtgcacgg gagtgctctt
      661 tccgtacatc gaggggacga gccgtccgga tacggccagg agcgcatcac ggattgcctc
      721 ggcctccaga cgccgcacgt tcattctgtg tagaaacgta ttggtcgggt ccagacgctc
      781 gaggtcgcca tggggagtgc tcaccatgcg gtatgtgctt gacgtgacca ttagtcgatg
      841 tatcgccttc caggaccagt catgcgcgag caactcggaa accagccagt cgagcagctc
      901 tgggtggctc ggcttttgcc ccatcgcccc gaaatcgtcg gtcgacttga cgatgccctc
      961 gccaaagtga tgcttccaga gtcggttgac cagcacgcgg gacaagagag ggttgagtcg
     1021 gggatcgacc attctccggg ccaaatacag gcggccgctt cccgcactcg tggcagaatc
     1081 ggaaccgccg agaatctcca ggaatcgccg gggaacaatt tcgcccaagt tcttgtggct
     1141 gccccgaatg tggattcgct cgtcctgacc cgtcccgtcg gcgattgcca gagccagcgt
     1201 cgggtcggga atttgttgct ctgtcgcgcg tacttcttca acggccgccg ccaggcggtc
     1261 ggcggctgcg tcatgggccg ttgcatgcaa ggccttgatc accccagcca gctcaagcgc
     1321 gggctcggtc accccgggca gctggtcgga gtcgctcccg actggtggcg cgggagttga
     1381 ccggttcgat agtcggattt cgtcgaccgc aatgtagccg cggccttctt cgatctgggc
     1441 gctgccaccc ccgaagtcga ctgtagaacc gtcgctgatc tcgaaatagg cggagtggcc
     1501 gatccacatg ccaagatctt gagtcaccca gcgtggatgg tcaccggcat cgattctgcg
     1561 cgtcaactca ccgtagatcg ggtcgcggat tttctcgaag ccgtcaacaa cgacgctgat
     1621 cctgcctccc tttcctgcga ccagccagtg gacataacgg ttctcgatcg tgaagctgcg
     1681 cgagcgcagc accccccgaa gccggtccga gatcaagccg ctgtgcgcct ggccgggttt
     1741 gattgagacc aggcgtgagc ccccgtttac cagctccagt cgcagaccac cggcccgaga
     1801 aggaccgtca ccgaaagcat caccggtgac cgaccaacca tcgaagctat cgccatggaa
     1861 atcttcgaat accgtctcat cttcgtcgct ttttggtgat cggctcgggc gggttgtctg
     1921 ccgggcggac gattcatcgc tcgcgcaccg ggtccagaca gtcgccgtct cgcggctaaa
     1981 cacctcgggc gcctgggtca ttgcctcgcg aatgagcgag gcaatcgttg atttgagact
     2041 caagagccgc ccgaccgcag gagcgaaccg ctggggagga tcgataaacg cttgctgatg
     2101 ccgcgagctg cgaagaacac ccgccaaggc gtaataatct ttgttggtga tcggatcgaa
     2161 cttgtggtcg tgacaccgcg cgcacgcgac ggtgagtccc agaaacgcct tggacatcac
     2221 atcaatctgg ttgtcgatcc gccgcatctg ttcttcgcgc acgtcgaccg gcgaatgcgt
     2281 cccttcacca aggaagtaaa acccggtccc gaggatggat tcgttgcttc ccgcgcctgc
     2341 ggaccgtcgc ggcgggtcga gcaggtcgcc ggcaatctgt tcggtcacga atcgatcgta
     2401 ggagaggtcg gcgttgagcg cgcggatcac atagtcgcgg tagccgaagg cattgacgat
     2461 gtcgtaatca aactcgtggc caccggtctc ggcgtagcga accaggtcga gccagtggcg
     2521 ggcccatcgt tcgccgtaac gggggctcgc cagcaggcga tcgacgagcc gctcgtaagc
     2581 atcggcgcca gcatccgcaa cgaaggctgc gacttcggtc ggactcggag ggagccccaa
     2641 gaggtcaaag ctcagccgac gaatcaaggt ccgccggtcg gcctccggcg acggtgccag
     2701 cccgtgttcg gtcatcgcag actggatgaa ctgatcgatc ggattgcgtg cccagtctag
     2761 ggggccggtc ttcaaggcgg gtggcgtcac ttgctttatg ggctggaacg accagaatcg
     2821 agcccgcttt tggtattctt ctctgctgag ttggtcggac ttgtcgctcg gtttggcatc
     2881 cgctgcattc gcggtagcaa cgggcgtctg gattccccag ggagcgcctc gcttgaccca
     2941 ctcggtcaat gcggcaatct cagcgtcggg gagtttcgat ttgggaggca tctggagtgc
     3001 ttcgccatag ttgatggcgt cgacgagcag gctctccgct gggttgccgg gcacgagagc
     3061 cggaccggtc gatccgccaa ccaggagcgc cgtacgcgca tcgagccgga gcccaccctt
     3121 ctgcttggcg
//
LOCUS       DATAQL010000050         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1531941, whole genome shotgun sequence.
ACCESSION   DATAQL010000050 DATAQL010000000
VERSION     DATAQL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531941"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VHS97_00805"
     CDS             complement(<1..481)
                     /locus_tag="VHS97_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3446759.1"
                     /translation="MEQRKKIPAEAVDLYTRFIHGEISRRDFFNGVERFAVAGLTATA
                     IVDALMPNYALGQQIRKDDERIKASYETVPSPDGNGYIRGYFVRPFSADSRDATPAKL
                     PGVIVIHENRGLNPHTEDVARRFALENFMAFAPDALTPFGGYPGDDYKGGQLFTKLDP
                     "
     gene            complement(669..>1942)
                     /locus_tag="VHS97_00810"
     CDS             complement(669..>1942)
                     /locus_tag="VHS97_00810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX3446760.1"
                     /translation="DTAALMAQDIASGNFTVLAENYKADISDFLSDPETNVVQAYATN
                     YLRNEWTALDRIISADLAYLKAKLKGDISVTSQTDANDAWIVEVDSTTASIATYRYDR
                     TTKALAKLFVSRPELEGETLAAMIPIEIQSRDGLTLVSYLTLPPASDPNGNGRPDKPV
                     PMVLLVHGGPWSRDTYGYYGLHQWLANRGYAVLSVNFRWSTGFGKAFVSAGDLEWGAK
                     MHDDLIDAVDWAIAAGITTTDKVAIMGMSYGGYATLVGLTFTPDRFACGVDIVGPSNL
                     STLLDSSPAYWTADEHSVQKRIGDPTTEQGRQLLHDRSPLFKVEAIKRPLLIGQGAND
                     PRVNQAESDQIVNAVAKKGIPVTYVLFPDEGHGFVRPENRIAFYAAAEQFLAAFLGGR
                     AEPIDGAIKASSATVPHGAAFTPGLAEALAQ"
BASE COUNT          386 a          588 c          579 g          389 t
ORIGIN      
        1 cggggtcgag cttggtaaac agctgcccgc ccttgtagtc gtcgcccgga taaccgccga
       61 acggcgtcag cgcatcgggc gcaaaggcca tgaaattctc cagggcaaag cgccgtgcaa
      121 cgtcctccgt gtgcggattg aggccgcgat tctcgtggat gacgataact ccaggcagct
      181 tcgccggagt cgcgtcgcgt gaatcggcgc tgaatggccg gacgaaatag ccgcggatgt
      241 acccattccc atccggcgag ggcacggtct catagcttgc cttgatgcgc tcgtcatcct
      301 tgcgaatctg ctgccccagg gcgtagttcg gcatcaacgc atcgacaatc gccgtggcag
      361 taaggccggc aacggcgaaa cgctccaccc cattgaaaaa gtcacgacga ctgatctcgc
      421 catgaataaa gcgtgtgtac agatctaccg cttccgcggg aattttcttc ctctgttcca
      481 taagaaacct cctcccttcg agttaaattc acgttgaggg taccattact cgcctgactt
      541 tcaagccctc gcgaaagaca gcaaggctcg gccattaagc ggtccgcggc tgagccagcc
      601 aaccgccgtc gcgaacgcga gaccgcagat gaggcggtga acttgcctga ggtgctgatc
      661 cggtccgttt actgcgccag ggcctcagcc aagcctggcg tgaacgccgc tccgtggggc
      721 accgtcgcgc tcgaggcctt tatcgccccg tcgatcggct cggccctgcc gcccagaaat
      781 gcagcgagga actgctcggc cgcggcatag aaggcgatcc tgttctcggg ccgtacgaag
      841 ccatggccct catccgggaa cagaacatag gtaacaggga tccccttttt cgccaccgca
      901 ttcacaatct gatcggattc cgcctggttg acgcgcggat cgttggcgcc ctggccgatc
      961 agcagcggcc tcttgatggc ttccaccttg aacagcgggg aacgatcatg aagcagttgg
     1021 cgcccctgct cggtcgtggg atcgccgatg cgcttttgaa cgctatgttc gtccgccgtc
     1081 cagtatgccg gcgagctatc gagaagcgtg ctcaggttcg acggcccgac gatatcgaca
     1141 ccgcaagcga acctgtccgg ggtgaaggtc agtccgacaa gcgtggcgta gccgccatac
     1201 gacatgccca tgatcgcgac cttgtcggtc gtcgtgatgc cggcggcgat ggcccagtcc
     1261 acggcatcga tcaggtcatc atgcatcttg gcgccccatt ccagatcgcc ggcggagacg
     1321 aacgccttgc cgaagccggt cgaccaacga aagttcaccg acaggacggc atagccacgg
     1381 ttggccagcc attgatgaag cccgtagtag ccgtatgtat cccgtgacca tggcccgcca
     1441 tggaccaaca gcaccatcgg aactggttta tcagggcgac cgttcccatt cggatcgctg
     1501 gccggcggca atgtcaggta cgacaccaga gtgagaccgt cgcgtgattg gatttcgatt
     1561 gggatcatgg ccgccaaggt ctcaccttca agctcggggc ggctgacgaa gagcttggca
     1621 agagccttgg tcgtccggtc gtagcgatag gtcgcaattg acgctgtggt ggaatcgacc
     1681 tcgacaatcc aggcgtcatt ggcgtcggtt tgcgaagtaa cggagatgtc gcctttcaac
     1741 ttggccttca ggtaggcgag gtctgcgctg atgatccgat cgagagctgt ccactcattg
     1801 cgcaggtagt tcgttgcata agcctggacc acgttggttt ccgggtcgct gagaaaatca
     1861 ctgatgtcgg ccttgtaatt ctcggcgaga acggtgaagt ttccgctggc aatgtcctgg
     1921 gccatcagag ccgcagtatc cc
//
LOCUS       DATAQL010000051         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466 k141_5621,
            whole genome shotgun sequence.
ACCESSION   DATAQL010000051 DATAQL010000000
VERSION     DATAQL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5621"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VHS97_00815"
     CDS             <1..720
                     /locus_tag="VHS97_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3446761.1"
                     /translation="CHYEFWPWRDKDIDAILKTNRELGLTAAQFSESPVKGFGYGITN
                     PDPGRRAEFEEEIRSAVPVAKKLGVKKLCVVAGEETKGYSREEQTEAVIAALKAGARI
                     VEPEAITLILEPLNVLVDHPRQLIVHSGEAATVLKAVGSPNVKMLFDVYHQQISEGNL
                     TGNIRKYHDLIGYFQIADHPGRHEPTTGEINYPFVLRTIHEIGYRDPIGMEMSPKSDP
                     MEAFKAIRHVDTLARGPLRTC"
     gene            840..>1915
                     /locus_tag="VHS97_00820"
     CDS             840..>1915
                     /locus_tag="VHS97_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446762.1"
                     /translation="MNGNSIKQSSILLRRVDHDFARFWSVVCANPERFVLGLGFFLRV
                     YIYLRNRTFWMDEASLWRNLAGMPILDFSAGLTGDQLAPIGFLILERALMALVGTSQY
                     ASRFLPLCCGLIALYLFSSLAQRLLSRQAALVALILFAFSDDLTHYSSELKPYSMDLA
                     VGLFFTLMALDAIDKPITWRTTAVLAISVVTAPWLSFASAFVVAGCGMILIVSSLLAG
                     RLRDAAVFIAIGIGWLASFAVSYRASAALLSPYTTMYAFWYFAFLPVWPLPMDAARLA
                     SAAGILLEIFVNPLNLVAPLWPRLGVAIPIALLFLGGISLLRRSPFAWGILVLPIGLA
                     MIASAMHRYPLHGRLILELVPAFYL"
BASE COUNT          332 a          580 c          554 g          449 t
ORIGIN      
        1 tgccactacg agttctggcc atggcgtgac aaagacatcg atgcaatcct gaagacgaac
       61 cgcgaactcg gcctcacggc cgcccagttc tcagagtcgc ccgtcaaggg gttcggatat
      121 gggatcacca atcccgatcc gggtcggcgc gccgagtttg aagaggaaat ccgatccgcg
      181 gtgccggtgg caaagaagct gggagtgaag aagctctgcg tggtggccgg cgaggaaacc
      241 aagggctatt cccgcgagga acagacagag gccgtcatcg ctgccctcaa ggcgggtgcc
      301 agaatcgtcg agcccgaagc gatcacgctc attctcgaac cactcaatgt gctggtcgat
      361 catccccggc agttgatcgt ccactccggc gaagccgcca cggtcttgaa ggccgtcggc
      421 tcgccgaacg tcaaaatgct cttcgatgtc taccatcagc agatcagcga gggaaacctc
      481 acgggcaata ttcgcaaata tcacgacctg atcggctatt ttcagatcgc cgatcatccc
      541 ggccgccacg agcccacaac cggagagatc aactatccct ttgtgctgcg gaccatccac
      601 gagatcgggt accgcgaccc catcggcatg gaaatgagcc ccaagagcga tccgatggag
      661 gcgttcaagg cgatccggca cgtcgatact ctggcgcgtg gcccactgcg cacgtgctga
      721 gcgtgcctgg agccgcgcac ctcggccgga gggcgtccgg cgcatatttt cggcaccgtg
      781 atcgccggcg cccccaacaa gagctggctc aaccttttgt cgtctcggaa cggatgagga
      841 tgaacggcaa ttcgatcaag caatcatcga ttttactgcg gcgggtcgac catgacttcg
      901 cacggttttg gagtgtcgta tgcgctaatc ctgagcgatt cgtcctcggg ctgggctttt
      961 tccttcgcgt ttatatctat ttgaggaatc gcacattctg gatggacgaa gcgtcgttat
     1021 ggagaaatct cgcgggcatg ccgatcctcg atttctccgc gggcctgacc ggtgatcaat
     1081 tggcacccat cggtttcttg attctcgagc gcgctttgat ggcactagtg ggaacttcgc
     1141 aatacgcctc acgattcttg cccctctgct gcggcttgat cgcgctttat ttgttctcga
     1201 gcttggctca gcgtctcttg tcgcggcagg cagcgcttgt ggccttgatc ctgttcgcgt
     1261 tttcggacga cctgacccat tattcgagcg agctcaagcc gtactcaatg gacctcgcgg
     1321 tcgggctctt cttcaccttg atggcgctcg atgcgataga taagccaatc acgtggcgta
     1381 caactgcggt gctggcaatc tcggtcgtca ctgccccgtg gctttcgttt gcctctgcct
     1441 ttgttgtcgc cgggtgcggc atgatcttga tcgtctccag ccttcttgcg ggccgtctac
     1501 gcgacgccgc ggtttttatc gcgatcggaa tcgggtggtt ggccagcttc gcggtgtcgt
     1561 atcgggcgtc ggcggcgctg ctcagtccgt ataccacgat gtatgccttc tggtatttcg
     1621 ccttcctgcc tgtgtggccg ttaccgatgg acgctgcacg actcgcttcg gccgccggga
     1681 tactgctgga aatcttcgtc aaccccttga atctggtcgc cccgctctgg cctcggctag
     1741 gagtcgccat tccaatcgcg ctcctgtttc tgggagggat ttcgctgttg cgccggtcgc
     1801 cgtttgcctg gggaatcctg gtcttgccca tcggcctggc catgattgcc tcggcgatgc
     1861 atcgttatcc gctgcacggc cgcttgattc tcgagctggt cccggccttt tacct
//
LOCUS       DATAQL010000052         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_157890, whole genome shotgun sequence.
ACCESSION   DATAQL010000052 DATAQL010000000
VERSION     DATAQL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157890"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1757)
                     /locus_tag="VHS97_00825"
     CDS             complement(213..1757)
                     /locus_tag="VHS97_00825"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF010094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HEX3446763.1"
                     /translation="MPNSLIESYLKSKDPAAIAPGFLAYLANLECVARVAPDVAKSIV
                     QELADQRSNIKMIASENFSSLATQCAMANLLTDKYAEGIPHHRFYAGCDNVDSIEDQA
                     NLRARELFGAAHAYAQPHSGADANLVAFWAILRARVELDEMAKILGAESPEALVPADW
                     YKLTGDQWGVVRKALGSQRLLGMDLASGGHLTHGYRLNASGKMFEAHGYTVDRTTFLL
                     DYDAIEKQALEVKPLILLAGFSAYSRNINYARMRAIADRVGALFMVDMAHFAGLVAGK
                     VLQGEFNPIAYAHIVTTTTHKTLRGPRGGLVLCTNELKEHVDRGCPLVLGGPLPHVMA
                     AKAVAFTEALRPEFATYAQRIVANARALGEAFVREGLNVLTGGTDNHLLVLDVASTHG
                     ITGRQAEDAIRRCGITLNRNPIPFDPNGPWYTSGLRFGAAAVTTLGMGKAEMNEIAAV
                     VALVLKNIKPSATKSGAIDKTKYTLDETIRGQAHSRVNNLLESYPVYPEIDLPFLVEG
                     LGMKSS"
     gene            1949..2860
                     /locus_tag="VHS97_00830"
     CDS             1949..2860
                     /locus_tag="VHS97_00830"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3446764.1"
                     /translation="MALEFAPGKTRIGWIGTGVMGRSMCGHLIAAGYEATVYNRTAEK
                     ARPLVEQGAKLADSPRGVALQSDIVFTIVGYPRDVREVTLGANGTLAGAKPGTVLVDM
                     TTSEPALAIEIDQAARAKQVHAVDAPVSGGDVGAREARLSIMIGGEKDVVDALTPLFE
                     TMGKTIVHQGPAGAGQHTKMVNQILIASNMIGVCEALLYAFKAGLDLNTVLQSVSSGA
                     AGSWSLSNLAPRVIAGNFEPGFFVEHFLKDMAIALDESRRMKLSLPGLALAQQLYLAV
                     EAQGYARKGTQALALALSRLSAVDWKQ"
     gene            complement(2981..3271)
                     /locus_tag="VHS97_00835"
     CDS             complement(2981..3271)
                     /locus_tag="VHS97_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3446765.1"
                     /translation="MRDFAAMDGVRIKTLIGIDSRRFDKAVAEIKERQGTAPGIVFGD
                     FRGVLDDKDIDALVVGTPDHCHAIPTVLGCQAGKHIYVEKPDAHNRRQSRQF"
     gene            3487..>3676
                     /locus_tag="VHS97_00840"
     CDS             3487..>3676
                     /locus_tag="VHS97_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446766.1"
                     /translation="MILFFLLLIDPPSFRHPRGRVVLFRGITPLCLVRDGRPRLGAAL
                     RLCKTHQSFMQRTVIGSAL"
BASE COUNT          712 a         1120 c         1148 g          696 t
ORIGIN      
        1 tgacgcgccg agagtggcaa ggtcacgtat gcattgctac catattggtc acgtggcagc
       61 ggcaggaaat gaattcggta gtcgttcagc ggaattccga gaccgcgaat atgcgaacaa
      121 tgaggaaacc aggaaaccag gaactgacca aacctgcctt ctcctgcctt cctgcattcc
      181 tcattcggtc cctgccgcat ttggctgaaa agttacgacg atttcatccc gagtccctcg
      241 acaagaaacg gcaaatcgat ttcgggatag accggatacg actcgagcag attgttgacg
      301 cgggaatggg cctggccgcg aatggtctcg tcgagcgtat atttcgtctt gtcgatcgcg
      361 cctgacttgg tggcgctcgg tttgatgttc ttgagcacca gggcaaccac ggcagcgatt
      421 tcgttcattt cggcctttcc catacccaga gtggtcaccg cggcggctcc gaaccgcaga
      481 ccgctggtgt accaagggcc gttggggtcg aaggggatcg ggttgcgatt gagggtgata
      541 ccgcagcgcc tgattgcgtc ttcggcctga cggcccgtga ttccatgagt cgaagccacg
      601 tcgaggacca gcaaatggtt atcggtgccg ccggtaagga cgttcagccc ctcgcgcacg
      661 aacgcttcgc cgagcgcccg ggcattggcc acgatccgct gggcgtatgt ggcaaactct
      721 ggccggagcg cctcagtaaa cgcgacggcc ttggccgcca tcacatgtgg aagcggtccg
      781 ccgagcacca gcgggcagcc ccggtcgaca tgctctttga gctcattcgt gcaaagcacc
      841 aagccaccac gcggaccgcg cagggtcttg tgcgtggtcg tcgttacgat atgagcatag
      901 gcgattggat taaactcgcc ctgcaacact ttccccgcaa ccaatccggc aaagtgagcc
      961 atgtcgacca tgaagagagc gcccacgcgg tcggcgatcg cgcgcatccg ggcatagttg
     1021 atgtttcgcg aataggcact gaaaccggcg agcaaaatca gcggcttcac ctcgagggcc
     1081 tgtttctcga tcgcgtcgta gtcgagcaga aatgtcgtgc gatcgaccgt atagccatga
     1141 gcctcaaaca tcttgccgga cgcattgagt ctgtacccgt gcgtcaagtg gccgcccgag
     1201 gcaagatcca tgcccagtag gcgctggctg cctaatgcct tgcggacgac accccactgg
     1261 tcgcccgtca gcttgtacca atccgcgggg acaagggcct ccgggctctc ggcacccagg
     1321 atcttggcca tctcgtccag ctcgacgcgg gcgcggagga tcgcccagaa cgccacgaga
     1381 ttggcgtcgg cgccgctgtg cggctgggca tatgcgtgcg ccgctccgaa tagctcgcgc
     1441 gctcgcagat tcgcctgatc ctcgatcgag tcgacattgt cgcagccggc gtaaaaccgg
     1501 tgatgaggaa tgccttcggc gtacttgtcg gtaagcagat tggccatcgc gcactgcgtc
     1561 gccaacgatg agaagttttc actggcgatc atcttgatat tgctgcgctg gtcggcgagc
     1621 tcttgaacga ttgatttcgc cacatcggga gcaacccggg ccacgcactc gaggttggcg
     1681 agataggcca ggaaaccggg cgcgatcgcc gcggggtctt tggacttcaa gtagctttcg
     1741 atcagggaat tcggcatgag tgggctcgag tgtgcaggga tcgggaagcg acggatagtc
     1801 cattttccgg ctgagacctg aacgtccccg gggccaggga ccggtcgggt gtgcggcccg
     1861 cggcccgtcg ttataatgtg tcgcggaagc ttgtgaattg taacggctaa caacgacctg
     1921 gctcaaggga acacgcgggg aacacgcaat ggcactcgag ttcgcgccgg gaaaaacgcg
     1981 catcggctgg atcggcacgg gcgtgatggg aagatcgatg tgcggccacc tgatcgcggc
     2041 cggctacgag gcaacagttt acaaccgcac cgccgagaag gcgcggccct tggtggagca
     2101 gggggccaag ctcgccgatt caccccgggg ggtcgccctc cagtctgaca tcgtcttcac
     2161 gattgtcggc tacccacgcg acgtgcgcga agtgacgctg ggcgcaaacg gcacgctcgc
     2221 cggagccaaa ccgggcaccg tcttggtcga catgacgacc agcgagccgg cgctggcgat
     2281 cgagatcgat caggcggcgc gggccaaaca ggtgcatgcc gtcgacgcgc cggtctcggg
     2341 cggcgatgtg ggcgcgcgcg aggccaggct gtcaatcatg atcggcggcg aaaaggacgt
     2401 cgtcgatgcg ctcacgccac tctttgagac gatgggcaag accatcgtgc atcaaggacc
     2461 cgccggcgca ggccagcaca ccaagatggt caatcagatc ttgattgcct ccaacatgat
     2521 cggcgtctgc gaggccttgc tctatgcgtt caaggccggt ctcgacctga acaccgtcct
     2581 ccagagcgtc tccagtggcg ctgccggctc gtggagtctc tccaacctcg cacccagagt
     2641 gatcgccggc aacttcgagc cgggtttctt cgtcgagcat ttcctcaagg acatggcgat
     2701 cgcgctcgac gaatcgcggc ggatgaagct cagcctgccg ggcctggcac tcgcccagca
     2761 gctctacctg gccgtcgaag cgcaaggata cgcacgcaag ggaacacaag cattagcgct
     2821 cgcgctttcc cgtctctcgg cagtcgactg gaaacagtag gcagcaggca gtaggcggtt
     2881 tgaggaaacc gatgcccttt actgaccgct gtgcaaaggc ggtcaagttt ttcgaaccgt
     2941 agaatcgggg tcggggtatg gatgcgtccc acaccaaccc tcagaattgg cgtgattggc
     3001 gacggttgtg cgcgtcgggc ttctcgacat agatgtgctt gccggcctga cagcccagga
     3061 cggtcgggat cgcgtggcag tggtcgggcg tgccgacgac cagagcgtcg atatccttgt
     3121 cgtcgagcac cccgcggaag tcgccgaaga cgatacctgg ggcggtccct tgccgctctt
     3181 tgatctcggc gacagccttg tcgaaccggc gggaatcgat gccgatcagc gtcttgatcc
     3241 ggacgccgtc catcgcggcg aagtcgcgca tcagggtgcc ggcccgctcg acgaactcct
     3301 accggacgtc cggttcgcgt cgcatccctc cgcacgacgc aagaccgccg cgtgaactgg
     3361 ctgccaggga cctcccccgc caagccgtga gaatgaaaac cgcgcgaaat actcgcacaa
     3421 cacgccgacg ccgaacgaac tagttgtagc gagggtgatc ggcctggtga acccggggtc
     3481 cttccgatga tcctgttctt cctcttgctc atcgacccgc cttcatttcg ccaccccagg
     3541 ggccgggttg tgctgttccg gggaatcact cccctctgcc tcgtgcgcga cggccgtcct
     3601 cgattggggg ccgctcttcg tctctgtaag acgcatcagt cgtttatgca acgcacagtg
     3661 attggatcgg cattga
//
LOCUS       DATAQL010000053         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2295528, whole genome shotgun sequence.
ACCESSION   DATAQL010000053 DATAQL010000000
VERSION     DATAQL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2295528"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(657..1439)
                     /locus_tag="VHS97_00845"
     CDS             complement(657..1439)
                     /locus_tag="VHS97_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HEX3446767.1"
                     /translation="MWAETRVAVVTGGGRGIGRGIALSLAERGFALVINYRSDVLAAT
                     ATCREAEDRGSPSASLIHADIAQLDQGRRLLDETIRAQGRVDLWVNNAGVAPAQRSDL
                     LETTAESWDGVVGTNLRGPFFLTQSVAKAMIQLIAAGIVAEPQIVFITSISSTFASVS
                     RAEYCVAKAGLSMVAQLFAVRLADAGIRVYEIRPGLIETAMTQPVRKTYNDKITAGIT
                     PIRRWGTPDDVGRAVAAIAAGAFPYSTGAVFDVDGGLNLRIL"
     gene            1623..1696
                     /locus_tag="VHS97_00850"
     tRNA            1623..1696
                     /locus_tag="VHS97_00850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1657..1659,aa:Arg,seq:cct)
     gene            1763..1836
                     /locus_tag="VHS97_00855"
     tRNA            1763..1836
                     /locus_tag="VHS97_00855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1797..1799,aa:Arg,seq:tct)
     gene            1993..2703
                     /locus_tag="VHS97_00860"
     CDS             1993..2703
                     /locus_tag="VHS97_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446768.1"
                     /translation="MAEERLTEIPTSWTTISSAHSPGPKSQAAMEELVGRYHDAVTRY
                     IHLKVRDKHLADEVLQEFWTKLLTGKLAGADKSKGRFRDYLRTVLHRLIIDHFRTRKL
                     QPLPPGDLLDSARTDDDFDRVWREAVINRVWSRLETYQAATPKNRYATVLQLRRDFPK
                     ASIDEIAAKLGEMIKTQVTPEAFRKNLQRARAKFIELLVQELRETIHPTSTEDVEAEI
                     FDLGLGHLYRRYTNSTES"
BASE COUNT          651 a          883 c          834 g          599 t
ORIGIN      
        1 gatgttggga tgctgaagtg tttccgcact cctggcctcg cgcaagaatc gttgcagcaa
       61 ggtcgcgttg cgggccaacg tccgcgtgag gaccttgagc gcgacttcgt ttcctgtctc
      121 aacgtgcacg gcacgaaaga cggagctcat tccaccggct cctagcgggt ggacgatgcg
      181 atagctcccc atccgctgct cggataccgg acccgtgctc ggctgactca tcgaggttga
      241 ctcatccatt acgaattcgc ctcgagatcg gcctcatgaa aagtcagacg ctgccggagc
      301 acttggtcga gctggcaaaa atgactgcga caacgccaca tccggtgcgg accgagcggt
      361 ctgtaacacg cgcgtctgtg cgtgattttc ggatcctcaa tcatcgttga agacggtcag
      421 ccgtttgccg gctttttacc gatatggttg cgaggattca aacactgcct ctccaatccc
      481 gatccgcgac ggtgccgtct tatttctccc gtcgttgttt cgcaacaccc tcattctacg
      541 tcagccgatt aagcttagga cagcccccga taggggcgag aagatatagt tgcagccctc
      601 ggatcggtcg ggatggagtt gttaattcgt aaagcattac ttaagagaat gttatgtcag
      661 agaattcgca gatttagacc gccgtcgacg tcgaagaccg cacccgtaga atagggaaat
      721 gcgccggcag cgatcgcggc gacggcccgg cccacatcgt cgggagttcc ccaacgacga
      781 atcggggtaa ttcccgccgt gatcttgtcg ttataggtct tgcgaacggg ttgggtcatc
      841 gcggtctcga tcaagccggg acgaatttcg taaacgcgga tccccgcgtc ggccagccgc
      901 accgcaaaga gctgcgcgac catgctcaat ccagccttgg ccacgcaata ctcggcccgt
      961 gagacgctgg cgaaggtact cgaaattgaa gtgataaaca cgatttgagg ctcagcgaca
     1021 atgcctgcgg cgatcaattg gatcatggct ttcgcaacgg actgcgtgag gaaaaaagga
     1081 cctcgaagat tggtgcccac cacaccgtcc cagctttcgg ccgtggtttc cagcagatcc
     1141 gaacgctgcg cgggggcaac gccggcgtta ttgacccaga ggtcgactcg accctgtgcg
     1201 cggatcgtct cgtcgagtag tcgccgccct tggtcgagct gtgcgatgtc tgcgtggatc
     1261 aaacttgcac tgggcgatcc ccggtcttcg gcctcacgac aggttgccgt cgcggccagt
     1321 acgtcagagc gataattaat aaccagcgcg aagccccgct cggcaaggct caaggcgatg
     1381 ccgcggccaa tacccctgcc cccgccggtt accaccgcta cacgcgtctc cgcccacatt
     1441 ggtaaatact cctcggaatc agtaccaccg actggattca cctcgcctcg atcacggaag
     1501 tcgtcctata atgcttacga ttcaagatac cgaggccact ggagtgttca cacggggacc
     1561 gcgtcgcaaa cacgttgaca gcaaaccgtt tcatacttga gaatctcgta agcgaaaatt
     1621 tcgcgctcgt agctcaggtg gatagagcgg cggtttccta aaccgcaggc cgcaggttcg
     1681 aatcctgccg ggcgcacctt gaccgggtgc gggtagcctg ggctctgtga ggctgacacg
     1741 gcgaaatgcg ccgaccttgg ttgcgcccgt agctcagttg gatagagcaa cggttttcta
     1801 aaccgtcggt cgcaggttcg agtcctgccg ggcgtatttg ggtcgaacgt tcacggcata
     1861 tgttatactc cggcacaacg ggattgatgg gtttcacgtg aaacggagtg ccagagtcgg
     1921 ccagggccgt ctttcccgaa tcgaattgtc gacagaacgg atgagatgcc ccgtcaatct
     1981 cggatggcag cgatggcaga agagcgcctg accgaaatcc ccacgagctg gacgacaatc
     2041 agtagcgccc actcacccgg cccaaaaagc caggcggcca tggaagaact cgtgggccgg
     2101 tatcacgatg ccgtgacgcg ttacatccat cttaaggtgc gagacaaaca tctggcagat
     2161 gaggtcctgc aggaattctg gaccaagctc ctgaccggaa agctcgcggg cgctgacaag
     2221 agcaaggggc gatttcgcga ttatctgagg accgttctac accggctcat catcgatcac
     2281 tttcgaaccc gcaagcttca accgcttcct cccggcgatc tgttggattc ggctcgaacc
     2341 gacgacgatt tcgatcgcgt ctggcgtgaa gccgtgatca atcgcgtctg gtcgcgtctt
     2401 gagacctatc aagccgctac cccgaagaac cgatacgcaa ccgttctcca actgagacga
     2461 gacttcccca aggcgtctat cgacgaaatc gccgccaagc tcggcgagat gatcaaaacc
     2521 caggtcaccc ccgaagcttt tcgcaaaaac ctccagcgcg cccgcgccaa gttcatcgaa
     2581 ctcttggtgc aggaactgag ggagacaatc cacccaacga gcaccgagga tgtcgaagca
     2641 gagattttcg atctcggtct cgggcacctc tatcgccgct ataccaactc aaccgagagc
     2701 tgatcgcggt tttcgcgagt ccccgcaatc tacgacgctt cacataggca ccgccgggga
     2761 aaatccttcg tcgaggagct tttgcgtttg attaaaattc ccccgtcccc ttgacccact
     2821 cgcttcagca ggtagagtta aatcaagcca gtgtgcgcaa cgtcagcgaa aggcgtcgct
     2881 caccagggcc ttcgggaccg atttgattac cgcgatggat ggtacgcgcc gcgcgcgatg
     2941 gacaccagag aggagcggcg atgctgg
//
LOCUS       DATAQL010000054         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1226639, whole genome shotgun sequence.
ACCESSION   DATAQL010000054 DATAQL010000000
VERSION     DATAQL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226639"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1168
                     /locus_tag="VHS97_00865"
     CDS             227..1168
                     /locus_tag="VHS97_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX3446769.1"
                     /translation="MTPSVVSKATAPSPFLSLACETARRLGAVGVLILPEGPMDWDLI
                     RVQRSSEIAILVASSSQRQLELIRKAGLIAVEVEPTEVAISERISLALIEAVANDLLQ
                     AGARVVAVYSGFESEALDSLTVIRLGEHLERLTARDLRALETSVPFETLKAVVDVAIE
                     IGREGREGKAVGTLIVVGDARNVMSRTRQLGFDPFKGYRRKERNVRDFRVREAIKEIA
                     QLDGAFVVARDGTVEAACRLIDAPMSGLTLPKGLGSRHWAAAAITEVTKAVCVVVSQS
                     NGTVRVFQSGDVILRIAPMRHARAMKWQDADTEPAPS"
     gene            complement(1229..1483)
                     /locus_tag="VHS97_00870"
     CDS             complement(1229..1483)
                     /locus_tag="VHS97_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446770.1"
                     /translation="MIRLETPDRFGEQLCAGIAIRNSGQIGVPAEKTVTPRKELNRVG
                     HARNLYHVSDGNTAPVEPTNAADNDQQTGWGLASVVNTTT"
     gene            1535..1945
                     /locus_tag="VHS97_00875"
     CDS             1535..1945
                     /locus_tag="VHS97_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446771.1"
                     /translation="MKAFELLDEETVERAVLLAPALSPRYDLTAALRAVRRDVVVFWS
                     PLDVIVLGFGTRLFGTVDRVRTVSAGLVGFQIPAALAHDARQNREYRKLRQIRWHPRM
                     ATTGNFGGHLGPDCPFFLKKYVVPLFQFDESAIS"
BASE COUNT          496 a          843 c          872 g          558 t
ORIGIN      
        1 cagtcgacga cacgtcgtcc tggagcgggc tgctgccgga tacgcggcaa taggctgacc
       61 cgcatcgact cgcgttgtta acgctcgtga atcgaatttt gaggaccgcg gcaatcgtcc
      121 cggtggttcc acgcttgggc attggtcctc accccaccga tatccgtttg aatcccgtcg
      181 tcgttgggcc gcgccagaca agaccccgag ttcgaggctg caccctgtga ccccaagcgt
      241 cgtgtccaag gcgaccgcgc cttcgccctt tttgtcgctg gcgtgcgaga ctgctcgccg
      301 gctgggggct gtcggcgtct tgatcttgcc tgaagggccg atggattggg accttatccg
      361 ggtgcagcgc agtagtgaga tcgcgatcct ggtggccagc tcgtcgcagc gacagctcga
      421 gttgatacgc aaggccggcc tgatcgccgt cgaagtcgag ccgaccgaag tcgcgatatc
      481 ggaacggatc agcctggcat tgatcgaggc ggtggccaac gacttgcttc aagctggtgc
      541 gcgcgtcgtg gcggtttatt ccggattcga gtccgaggcg ctcgactcac tgaccgtaat
      601 ccggttgggc gagcacctgg aacggctcac cgcgcgcgac ttgcgagcgc tcgagacatc
      661 agtgcctttt gaaacgctaa aggcggttgt cgacgtggcc atcgagatcg gtcgcgaggg
      721 ccgcgaagga aaagctgtcg gcacgctgat cgtcgtcggc gatgcgcgca acgtgatgtc
      781 caggactcgc caactgggtt tcgacccatt caaggggtat cgtcgcaagg aacgcaacgt
      841 tcgtgacttc cgcgttcgcg aggcgatcaa ggagatcgcc caactcgacg gtgcctttgt
      901 ggtggcgcgg gacggtaccg tggaagccgc ctgccgtttg atcgacgccc cgatgtccgg
      961 actgacgctg cccaagggcc tgggctcgcg ccactgggca gcggccgcga tcaccgaggt
     1021 taccaaggcc gtctgtgtgg tcgttagcca gtccaacggc accgttcgcg tctttcagag
     1081 cggcgatgtc attctccgca tcgcgcccat gcgccacgcc cgcgcgatga agtggcaaga
     1141 cgccgacacc gagcccgctc cgtcgtgacg gacggaaaga atctcttggg tctagcggac
     1201 ggtgccaatg cacgacggat gcgacaaatc aagtggtggt gttgacaacc gaggccagcc
     1261 cccagccggt ctgttggtcg ttgtcggcgg cgttggtggg ctcgactggt gcggtattgc
     1321 cgtccgacac gtggtaaagg ttgcgcgcat gccctactcg gttcaactct ttccgtgggg
     1381 tcacggtttt ctccgctggc acgccgatct gaccagaatt acgaatcgcg atgcccgcgc
     1441 aaagctgctc gccgaaacga tcgggcgttt caagacggat catccgcgca gtccgatagt
     1501 cctggtcgcg aaatcgggcg gttcgggagt ttgtgtgaag gcgttcgaat tactggatga
     1561 agagacggtc gagcgcgcgg tgttgctcgc tccggcgttg tcgcctcgtt acgacctcac
     1621 cgccgcgctt cgcgcagttc gccgggacgt ggtcgtcttc tggtcgccgc tcgacgtgat
     1681 tgtactgggg ttcggcacgc ggttgtttgg cacggtcgat cgggtcagga cagtcagcgc
     1741 gggcctcgtt ggttttcaga ttcccgccgc cttggctcac gacgcgagac agaaccgcga
     1801 gtaccggaag cttcgtcaaa ttcgctggca cccgcggatg gcgaccaccg gcaatttcgg
     1861 cggacatctg ggaccggatt gtcctttttt tctcaaaaag tacgtcgtgc cactcttcca
     1921 gttcgacgaa tccgcaatca gttgaatcgg agttcagggt caagccgcta gggcggtgcg
     1981 ccgatatcct attggcctgg ccagcgccga tcgaaagaca cgtcgcgggc caggcagtcg
     2041 ctcagacacg atccgatccg tccggcccgc aaacgcggct tggtcgcctc aatcgcgacc
     2101 gggcgtgggt cggcgggagg ccgggtctga tcggcgtctg agcctccgga tacgggctta
     2161 aaaccggtcg gaatttcaag agcgtccggg ccgccacccg gggaggccgc aacgacttcc
     2221 ccggacgacg ccccgggcgc tctgaacgtc gagggtctcg agcactctcg cctgggaacc
     2281 aatcccggac taacgccctc gagaactggc acccttgaag ccgcgccgcg cggcggtctc
     2341 gccttttcaa aagggcgacc cactctctgc ccgttgacgc atcgagaggg cgggaccgcc
     2401 tcgcacttga ccttcctcgc aatatctcga acccggcatc acgtgacgac gctataacag
     2461 acctgttttg ctcggctctt gactttactc aataccagat ttggaattgc agtcaggatt
     2521 cgacctttcc tcaagagcgc tgcgttcact ttggagcgat ggcgcacttg accctacggg
     2581 gcttgcaccg gataaatctc gttatcgaga gtcgctcgaa tccaccgggc aactgggaac
     2641 gttttcccct tgtacgcgaa aaggctcgtc atcgaaaagc ggacgagact catcgccggg
     2701 ggcttcgcaa ttattggagt tagaagaaat caatgaacgg ccagcttgct cggatcgcgc
     2761 acgtccacg
//
LOCUS       DATAQL010000055         3911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4738060, whole genome shotgun sequence.
ACCESSION   DATAQL010000055 DATAQL010000000
VERSION     DATAQL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4738060"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..541)
                     /locus_tag="VHS97_00880"
     CDS             complement(50..541)
                     /locus_tag="VHS97_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446772.1"
                     /translation="MVVCSGSVHHVREIERFFRVVKGALTSDGVFVFNEYVGPCYQVY
                     PSEQLDVVNRLLRAIAPDFRRRQKLDQTTVEFMLARDPSEAVRSSLILPFASAYFDIE
                     LCRPYGGTVLHPLYPLLRHDAMAKSTPEIQTVMRLLIEVEAILVEKGVLASDFVLCVC
                     RKK"
     gene            complement(566..925)
                     /locus_tag="VHS97_00885"
     CDS             complement(566..925)
                     /locus_tag="VHS97_00885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3446773.1"
                     /translation="MILSKAQSYWERNPEIASEDQWTGNPLIAEAVYRRISAGESSDH
                     WLAWLFRDYFKKQQFARVLSPGCGAGDHEVAMISLGCINQLDAFDFSEASLGIAREKA
                     AAKNLNIDFYFDDINSF"
     gene            complement(1393..>3911)
                     /locus_tag="VHS97_00890"
     CDS             complement(1393..>3911)
                     /locus_tag="VHS97_00890"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3446774.1"
                     /translation="HSENGAPKEEPKDLRLLYPAARALKDQEKRERLRLPAGGMVWIT
                     AAGSPLTDAVCAALKKQDLRSQVVRLDQETISQPDESLCGLIILAPREPHSQTFVKDA
                     FRVLRAAGPALQQTAARESALLLTVSRFDGTFGLNGLALESSPAAGALAGLAKTARHE
                     WQGVDCKALDLDRSIESPTEAANAIVDEIVHRGAIEVGLKSDGARVCIDLEPASSGGS
                     HNGVHNGRFVPLRRDDLVVMSGGARGVTAEVAVALAKSFGPRLVLLGRTPAPAAEPDW
                     MADIRDDAQLKRVLLDRSNGRRSPHELGEETRRLLTEREIRHNLTRIEEAGSQVIYRS
                     IDVRDGAAVRAILQEMQAKYGKIRGLIHGAGVVADRKIIDQTDAQFDAVYDTKVLGLD
                     HLFQAIDPQALSFLILFSSSTARFGRTGQVAYAAANEVLNKWAQQQSLRLPHCRVISY
                     NWGPWAGGMVQDSLKPLFEKEGIALIALEAGARCVVDQIKKGSPTAVEVVVLAEHNPT
                     ASVAAAPTPKSTATLPAERKPEPVFRRAVDLESLPVIASHVIDGHPVLPMAIMLEWLA
                     EGAIHRNPGLVVSGIDDLRLFKGVILNHSPQASVEVRVGKAVRHGSQFVVPTELKGIM
                     AGGREMAHARADVILTDRHSTGARKLAETTLIPYPHTCAEIYRTILFHGPALQGIDKV
                     EGLGDHAVSGWAATAPEPAEWLDRPLRSAWLTDPLAIDCAFQLVVLWCRNRLGANSLP
                     TAIGGYRQYRRSFPAAGVRIQAEIQHSSDTRVVADIEFLDGHGDLVARLESYECVVDA
                     SLNQAFRRNQIAQAITVPPLVKGGPGGVVRALSDTSQPKY"
BASE COUNT          740 a         1141 c         1265 g          765 t
ORIGIN      
        1 cggcattcaa atgtagtccg tcttctagcg aggctcgagt gcgtttgctt catttcttcc
       61 gacaaacgca cagcacaaag tcactggcga ggacgccttt ttcgacgaga atcgcctcga
      121 cctcgatcaa caggcgcatg actgtttgta tctctggcgt cgatttcgcc atcgcatcgt
      181 gtctcaagag aggatacagc ggatggagca cggtgccgcc atagggacgg cagagctcga
      241 tgtcgaagta ggcggatgca aacggcaaga tgagcgacga tcgaaccgct tccgacggat
      301 ctctggccaa cataaattcg accgtggtct gatcgagctt ttggcgacgc ctgaaatcag
      361 gtgcaatcgc ccggagcagg cggttgacga cgtcgagctg ctcgctgggg tagacctggt
      421 agcagggccc gacgtattcg ttgaacacga acacgccgtc cgatgtgagc gcgcccttga
      481 ccacgcggaa gaaccgttcg atctcgcgga cgtgatggac cgatcccgaa cagacgacca
      541 tgtcatactt gcgggcggcc gggacctaga atgaattgat atcatcaaaa tagaaatcga
      601 tattgagatt cttggcggcg gctttttcac gagcgattcc caggctcgct tccgagaagt
      661 caaaggcgtc cagttggttg atgcagccga gcgatatcat ggcgacctcg tgatcgcctg
      721 cgccgcaccc cggagagagg acgcgagcga actgctgttt cttgaaatag tcacgaaaga
      781 gccaggcgag ccagtgatcg cttgactctc ctgccgaaat ccgtcgatag acggcctcgg
      841 cgatcagagg attgcctgtc cactggtcct cggaagcgat ctccgggttg cgctcccagt
      901 acgactgtgc ttttgacagg atcatgggcc gtcttcggtt cgccgagccg gtgccccgat
      961 actgtgtcga ccatcgcctt atcgtcgaca ggatcatggc tactcccaga ttcaccggac
     1021 agtgtcattg gcctgggatc aaaatatggc tcggtggacg agcccaggat cgggaatcta
     1081 gcggattgtt aagaaaagtc aaggccgctc ggacgcggtt ggttgcgcca acggattctg
     1141 catcataggc gcgggaagtg ggcggtgtgg ggaccggcac cctcggactg tgggcgtgca
     1201 gaccttcagc ctggcgtgcc ggcggggtcg gattcgcccg gactgagcaa taggcggact
     1261 tcggccttca cgaggggggg aaaggatgct gggttgtcaa tgttttggtc gcgatgtgtg
     1321 tgtgcgtgtg ttagcgcctt gaccaccgct ccccggcccc ccctcacatg gggaggaaac
     1381 gatgccgtgt cgtcaatatt ttggttgcga tgtgtctgat agcgccctga ccaccccccc
     1441 cggccccccc ttcacaaggg ggggaacggt gatcgcttga gcgatctgat tacggcgaaa
     1501 cgcctggttg agcgaggcat cgacgacgca ctcgtacgac tcgagccggg cgacgagatc
     1561 accatgtccg tccaagaatt cgatgtcggc gacgactcgc gtatcgcttg agtgctggat
     1621 ctccgcctgg atgcgcacac cggcagcggg aaagctgcgg cgatactggc gatagccgcc
     1681 gatggctgtg ggcagcgagt ttgcccccag gcgattgcgg caccagagga cgacaagctg
     1741 aaaggcgcag tcgatggcca ggggatcggt cagccaggcg cttcggagcg gccgatcgag
     1801 ccattccgcg ggctcgggtg cggtggcggc ccagccgctg accgcgtggt cgcccaggcc
     1861 ttcgactttg tctatgccct ggagcgcggg gccgtggaac aaaatggtgc gatagatctc
     1921 tgcgcatgtg tgcgggtagg gaataagcgt ggtttcggcg agcttccggg cgccagtcga
     1981 gtggcgatcg gtcaggatca cgtcggcccg cgcgtgggcc atctcgcgac cgccggccat
     2041 gattcccttg agctcggtgg gcacgacgaa ttgagaaccg tgacggaccg ctttgcctac
     2101 gcggacctcg acggacgcct gcgggctatg gttcagaatc acccctttaa agagccgtaa
     2161 gtcgtcgatc ccgctcacca cgagtcctgg attgcggtga atcgcaccct cggcgagcca
     2221 ttccagcata atcgccatgg gcaacacggg gtgtccgtca atgacgtgcg aggcgatgac
     2281 cggcaaagac tcgagatcca cggcgcggcg aaagaccggc tcgggctttc gttcggcggg
     2341 gagcgtcgcg gtcgatttcg gcgtgggggc ggcggctacg ctagcggtcg ggttgtgttc
     2401 ggcgagcacc acgacttcga ccgcagtagg gctgcccttc ttgatttgat cgacgacaca
     2461 gcgagcgccc gcctcgagcg cgatcagcgc gatcccttcc ttttcgaaca gcggtttgag
     2521 ggaatcctgt accatgccgc cggcccaagg tccccagttg tacgagatca ctcgacaatg
     2581 gggcagacga agcgactgct gctgagccca cttattaagt acttcgttgg ccgccgcgta
     2641 ggccacctgg cccgtgcggc cgaacctggc cgtggaagac gaaaatagga tcaagaatga
     2701 gagcgcctgg ggatcgatcg cttggaagag atgatccagg ccgaggacct tagtgtcgta
     2761 aaccgcgtcg aactgagcgt cagtctgatc gatgatcttg cggtcggcca caacgccggc
     2821 gccatgaatc agaccgcgaa tcttgccgta tttcgcctgc atttcttgga ggattgcccg
     2881 aacggctgcg ccgtcgcgca cgtcgatcga ccgatagatc acctgcgagc cagcttcttc
     2941 gatacgagtg aggttgtggc ggatttcgcg ctcggtcaac aggcggcgcg tctcttcgcc
     3001 gagctcgtgg ggactgcggc gcccattgga ccggtccaag agcacgcgct tcagctgggc
     3061 gtcgtcgcga atgtccgcca tccagtcggg ctctgcggcc ggcgctggcg ttcgaccgag
     3121 caagaccagt cggggcccga acgatttggc caaggcgaca gcgacttcgg cggtaacccc
     3181 gcgcgctccg ccactcatca cgaccagatc gtcgcgccgg aggggaacga atcggccatt
     3241 gtgcacgccg ttatgcgagc ccccgctcga ggcgggctcg aggtcgatgc acacgcgagc
     3301 gccgtcggat ttcagtccga cttcgatggc ccccctgtga acgatctcat cgacgattgc
     3361 atttgcggcc tccgtcggtg attcgatcga gcggtcgaga tcgagcgcct tgcaatcgac
     3421 cccttgccat tcgtggcggg ccgttttggc caggcccgcg agcgctccgg cggccgggct
     3481 ggattccagc gcgagtccgt tgagtccaaa agttccatca aagcgcgaaa cggtcagaag
     3541 gagggcagat tcacgggcgg cagtctgctg gagggcagga ccggctgcgc gaaggacgcg
     3601 gaacgcgtcc tttacaaacg tctgcgaatg cggttctcga ggcgccaaga tgatcaaccc
     3661 gcaaagtgac tcgtccggct gcgaaatggt ttcttggtcc agccggacca cctggctacg
     3721 aaggtcttgt ttttttagcg ctgcacagac ggcgtcagtc aacggggagc cagccgccgt
     3781 gatccagacc atcccgccgg cgggaagccg gaggcgctcg cgtttttctt ggtctttgag
     3841 tgcgcgagcc gccggataga gcaaacggag atccttgggt tcttccttcg gcgcgccgtt
     3901 ttcgctgtgt t
//
LOCUS       DATAQL010000056         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2142842, whole genome shotgun sequence.
ACCESSION   DATAQL010000056 DATAQL010000000
VERSION     DATAQL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142842"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VHS97_00895"
     CDS             <1..446
                     /locus_tag="VHS97_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX3446775.1"
                     /translation="SFVELPLGAASAAPLKSGTWTLLWNVEQRLVVDPDDPDRGLGMY
                     VQTGLGDGNPNPVRWFVSAALSGNSPLPGREGDIFGVGFYDLGPSAQAKSQAPGLRDE
                     WGVELFYNLRIAPGCHLTPDLQVLHPGLAPLANALVFGIRLKVDF"
     gene            complement(574..>2112)
                     /locus_tag="VHS97_00900"
     CDS             complement(574..>2112)
                     /locus_tag="VHS97_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3446776.1"
                     /translation="AGLALLDHPSEHIRAWGVRLLCDGTTPAPPTLARFAELAKFDPS
                     PKVRLSLASAAPRLPVDARWAVVEPLLSHKEDATDRVLPLMAWYAVEPLVTADRSRAM
                     SVAAVCQIPTVRRFMARRTVEADPAAGLAAVLALLKTADNAGCFDLLIGTHDGLRGRK
                     HITRPEGWPEVFANLVARPSLAVVEQTLLLALDLGEPKAAAVLRQVVRDEKSPQAMRQ
                     RALSAIVERRLPGLVEDLQGLVDDSAMRGPVLRALAAYDDPATPEILLTRYGNLPESD
                     RNDAIATLASRPRWALVLLDAVERGRVPRRDLSIAVARQLQAFGDPRIGARLEAVWGK
                     IQPTSKSKAALVARYKSLLGPSQEPAADPSRGRALFNRTCLSCHKLFDAGGDVGPELT
                     GSDRANTDYILENVLDPSAAVSRDYTVTSIATADGRLISGIVREQTDASLVIQTANER
                     LVVPREDVEAIKPSKTSMMPEGLLDPLSPGEIRDLFAYLASSKQVSTPPDGRAPESTN
                     GLSR"
BASE COUNT          355 a          682 c          696 g          379 t
ORIGIN      
        1 catcgtttgt cgagctgccc ctaggggccg cctccgccgc gcccttgaag tccgggacct
       61 ggaccctgct ctggaatgtc gagcagcgac tcgtggtcga ccccgacgac ccggaccggg
      121 gtttgggcat gtacgtgcag acgggcttgg gcgatggcaa cccgaacccg gtccgctggt
      181 tcgtgagcgc agccctctcc gggaacagcc cgttgccggg ccgcgaagga gatattttcg
      241 gcgttgggtt ttacgacctt gggcccagtg cgcaggccaa gagccaggcg ccagggctcc
      301 gggacgagtg gggagtcgag ctcttttaca acctgcggat cgcacctggt tgccacctga
      361 cgccagacct gcaggtgctc catccggggc tggcgccttt ggctaacgcg ctcgtgttcg
      421 gcatccgcct aaaggtcgat ttttgatgac gccgcggctg ggtggcgctg gcgaatgctg
      481 ggccacttgc taccgctctc gatcgactca agcgatccgt ggcagcccga cctgaagcgg
      541 ccgtgccgct tgtcatcgtc atgactgcgt accttagcgc gaaagtccgt tggttgactc
      601 cggagccctt ccgtccggcg gcgtcgatac ttgtttgctc gaagccaggt acgcaaagag
      661 atcgcgaatc tcgcctgggg acaatgggtc gagcagtccc tcgggcatca ttgacgtctt
      721 ggaaggcttg atcgcttcca cgtcctcgcg gggtacgacc agccgctcgt tggcagtctg
      781 gatgaccaac gacgcgtcgg tctgctcgcg gacgatcccg gagatcaagc gcccatcggc
      841 ggtcgcgatg ctggtcacgg tgtagtcgcg gctcacggcg gcgctcggat ccaggacgtt
      901 ttcgagaatg tagtcggtgt tggcacgatc cgagccggtc aactcgggtc cgacgtcgcc
      961 gccggcgtca aagagtttgt ggcacgacaa acacgtgcgg ttgaagaggg cccggcctcg
     1021 cgacgggtcg gccgccggct cttggctagg gccaaggagt gacttgtacc tggccaccaa
     1081 ggcggctttc gacttcgatg tcggctggat cttgccccag acggcttcga gccgtgcacc
     1141 gatcctcgga tcgccgaagg cctgcaattg ccgcgcgacc gcgatgctca gatcacgacg
     1201 cggcacacgc ccccgttcca cagcatccag tagcacaagc gcccaccgtg ggcgcgaggc
     1261 tagagtggcg atcgcgtcgt tgcgatccga ctcggggaga ttgccgtagc gggtcaaaag
     1321 gatctcgggc gtcgcgggat catcataggc agccagcgcc cgcaataccg ggccgcgcat
     1381 ggcggaatcg tcgacaaggc cctgcagatc ttctaccaag ccgggcaggc ggcgttccac
     1441 gatggccgac aaggcccttt gcctcatcgc ttgaggactc ttttcgtcac gaacgacctg
     1501 ccgcaagacc gcggctgctt tgggttcccc caggtcgagc gccaggagca gggtttgttc
     1561 caccaccgcc agactgggcc gcgccaccag attggcaaag acttccggcc agccctcggg
     1621 acgggtgatg tgcttgcgcc cgcgcaggcc gtcgtgcgtg ccgatcaaga gatcgaagca
     1681 gcccgcgttg tccgccgttt tgagcaacgc gagcaccgcg gccagaccgg ctgccgggtc
     1741 tgcctccacc gtgcgccggg ccatgaatcg gcggacggtc ggaatctggc agacggcagc
     1801 gacactcatg gcccgcgacc gatcggccgt gacgagcggc tcgaccgcgt accatgccat
     1861 caagggaagc actcgatcgg ttgcatcttc cttgtggctc aacagcggct caacaactgc
     1921 ccacctcgcg tctactggca accggggtgc ggcagaggcc aagctgagcc ggaccttggg
     1981 gctgggatca aatttggcca gttcggcgaa ccgggccaat gtgggagggg ccggtgtcgt
     2041 cccatcgcag agcagcctca ctccccacgc ccggatatgc tcgctcgggt gatcgagcaa
     2101 tgccaggccg gc
//
LOCUS       DATAQL010000057         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4891060, whole genome shotgun sequence.
ACCESSION   DATAQL010000057 DATAQL010000000
VERSION     DATAQL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4891060"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VHS97_00905"
     CDS             <1..600
                     /locus_tag="VHS97_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446777.1"
                     /translation="QLLASRTAVAQWGMSPVIDATELRLLRIAYRRAHGVAEMPSPEF
                     LEELTHELKNQFPSNADLLNESELFYDFQGRARGSAKELDAHVITANDDTVCGDTDPL
                     RDRSPLAKNVCRKVGMVQNDLAKIQPGWFHVGRWRDIPKDACYVGRSGHVYVWVGPEG
                     LDQLTEFTLTVLKFTTLIKETQTLISPGSVKFSPGDRGG"
     gene            complement(658..1314)
                     /locus_tag="VHS97_00910"
     CDS             complement(658..1314)
                     /locus_tag="VHS97_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446778.1"
                     /translation="MDTGPDTIGQNATCARGSFVVSEERTTTIVQRYLDALAGDAPAE
                     PIVRELLDRAVHRLEKLCANMLHRSYPRLTGPPSNLETAELLGGVVEGLLKAMRSIRP
                     QTVRQFFALANQHMRWQLNDLARRLDERPPAVELREGLVPAAASSDSGITANGRRMLE
                     AIDSLPEDEREIFGLVRLQGLTQTEAALVVGVSARTVKRRLNRASLLLAEELDNLRPV
                     "
     gene            1518..2351
                     /locus_tag="VHS97_00915"
     CDS             1518..2351
                     /locus_tag="VHS97_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX3446779.1"
                     /translation="MKQPELYERFRALHEQKGGVVMPNAWDGVSALLLKQAGFMALGS
                     SSAAIAFSLGRIDGAHAVSAVEALENAALLGRLTGLPINADLEDGFGPAPEDCAATVL
                     AAIAAGMAGLGIEDTTANPEKPIHEFDHAVERIRQAARVAKGRILLTGRTDNYLFGRP
                     DLDDTIRRLVAFAQVGADVLYAPLLPDMDAIRAVVKAVAPKPVNVLIGPGTGPVPIAE
                     LQAAGVRRVSLGAALYCHAMASLEDAAFALAAGNIAPAAEGLRPSKIMGMLREATAKT
                     A"
     gene            2384..3343
                     /locus_tag="VHS97_00920"
     CDS             2384..3343
                     /locus_tag="VHS97_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3446780.1"
                     /translation="MIRTSCPLSLFAAITLVLGLDGAASAQQAVEVLHKTVKVGDLDI
                     FYREAGPKDAPTLLLLHGFPTSSQMFRKLIPKLADKFHVVAPDYPGFGHSSMPSRDEY
                     SYTFDNLSKTIDEFTGKVGLTKYALYVQDYGGPVGYRLAVAHPERITAIVVQNGNAYD
                     EGLDNDFWKPLKAYWKEPNSRERRDALRAFLKYDATKWQYTHGVKNPELVSPDGPAHD
                     QFLLDRKGNDEIQLDLFLSYGSNPPLYPKWQAYFREYQPPVLIVWGKNDQIFPAAGAE
                     PYKRDLTSLEYHLLDAGHFALETNGDEIARLMREFLGKQVAAK"
     gene            3438..>4319
                     /locus_tag="VHS97_00925"
     CDS             3438..>4319
                     /locus_tag="VHS97_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX3446781.1"
                     /translation="MRAILRTAFGGPEQLVIREIPKPEPKEGHVVIAVKAFGINHAEM
                     HMRKGEWAEIADVSGIECVGVVESCPGGEFTVGSKVAALMGGLGRTINGSYAEFTRAP
                     VTNVAAIESNLPWEDLAAIPESYATAWTCLFRNLEIASGQTLVIRGATSSFGQAALNM
                     AVNSGVRVIATTRNRGRFQTLEALGASRVEVEGPDLSNRLTEAKRVDAVLDLVGNSTI
                     LDSLALLRRGGRACLAGFLGGLAPVPDFNPLLQMASGVHFSFFGSFVFGTPGFPLSDV
                     PLAAIAGDVAAGRYKAKP"
BASE COUNT          837 a         1321 c         1359 g          802 t
ORIGIN      
        1 cagctcctcg cgtcgaggac cgccgtcgca caatggggta tgtcgccggt gattgatgca
       61 accgagttgc gcctgctccg gatcgcctat cgccgcgcgc acggtgttgc cgaaatgcct
      121 tcgcccgagt ttctcgagga gttaacccac gagttgaaga accagttccc atccaacgcc
      181 gacctcctga acgagtcgga gttgttctac gacttccagg gccgcgcccg cggcagcgcc
      241 aaggagctgg atgcccacgt gatcacggcc aacgacgata ccgtctgcgg cgacaccgac
      301 cccttgcgtg atcgctcacc gttggcgaag aacgtctgcc gaaaagtcgg aatggttcag
      361 aacgacctcg ccaagatcca acccggctgg tttcacgtag gtcgttggcg cgacatcccg
      421 aaggatgcct gctacgttgg gaggagtggg cacgtctacg tctgggtcgg tcccgaaggg
      481 cttgatcagc tcacggagtt cacgctgact gtgctcaagt ttactaccct gatcaaggag
      541 acacagacgc tcatcagccc cgggtctgtg aagttctcgc cgggggaccg cggcggatga
      601 ccatcgtcct cgtaccgggc ggtgactcgt caaatgcgag aatgaaacga ccctgtccta
      661 taccggccgc aggttgtcga gttcctccgc cagcaataga ctggcgcgat tcaagcgccg
      721 ctttacggtc ctggccgaga cgcccaccac gagggcggcc tcggtctggg tcaatccctg
      781 caggcgcacc aagccgaaga tctcgcgctc atcctccgga aggctgtcga tggcctccaa
      841 cattcgacgt ccgttcgctg tgataccgga atcgctgctg gccgcggcag gcacgagccc
      901 ttcgcgcagc tccacggcgg ggggtcgttc atctaagcgg cgagccagat catttagctg
      961 ccagcgcata tgctggttgg ccagggcaaa gaactggcgc acggtctgag gacgaatcga
     1021 acgcatggcc ttgagcaacc cttcaaccac gccgccaagc aattcagccg tctccaaatt
     1081 cgacggcggg ccggtcagac gtgggtagct ccggtgcagc atgttggcgc acaacttctc
     1141 caggcggtga acggcccggt ccaacaactc ccggacaatc ggctcggccg gcgcgtctcc
     1201 agccaaggca tccaggtagc gctgcacgat agtggtggtg cgttcttcac tcacgacgaa
     1261 tgacccccgc gcacaggtgg cattctgacc gatggtatcg ggtcccgtgt ccacctgcta
     1321 ctcttagcgc tgatggggac ggtcatcagc atgacagatc gaaattccca gggcgcttag
     1381 cattcaatcc gttggcgcac tcaggtcgcc gaaatatcgt cgtaaccaac aaacatcgac
     1441 ttgacaagtt agcagttttt caataacctt tatcacagga tttttacggt tatcgcttca
     1501 agcgaggagg aggtttcgtg aaacaaccgg aactctacga gcgcttccgt gccctccatg
     1561 aacagaaggg cggcgtggtg atgccgaacg catgggacgg cgtctctgcg ttgctgctga
     1621 agcaggccgg gttcatggcg ttgggatcgt cgtccgccgc aattgccttt tcccttggcc
     1681 gcattgacgg cgcgcatgcg gtcagcgcgg tggaggcgct ggagaatgcc gcgttgctcg
     1741 gcaggctgac tggacttcca attaacgccg atctcgagga cggcttcggc ccggcacctg
     1801 aagattgcgc cgcgaccgtc ctagcggcta tcgcggcagg gatggcgggg cttggcatcg
     1861 aggacacgac ggccaatcct gagaaaccga tccacgagtt cgatcacgcc gtcgaacgga
     1921 tccgccaggc ggcccgcgtg gccaaggggc gtatcttgct gaccgggcgt acggacaact
     1981 acctgttcgg caggccggat ctggacgaca cgatcagacg cctcgtcgct ttcgcccagg
     2041 tcggcgctga tgtgctttat gcgcctctac tgccggacat ggacgcgatc cgggcagtgg
     2101 tgaaggcggt ggcacccaaa cccgtcaatg tgctcatagg gcccgggacc ggtccggtgc
     2161 cgatcgctga actgcaagcg gccggcgtgc ggcgcgtcag cctgggcgcc gcactttact
     2221 gccatgcgat ggcgagcctc gaggacgccg cctttgcgct ggcagcaggg aacatcgccc
     2281 ccgcggcgga gggtctccgg ccgagcaaga tcatgggcat gctgagagag gcgacggcga
     2341 agactgcgta agggaagaca tcatcatgac tagagaggcg aaaatgattc gtacatcctg
     2401 ccccctgtcg ttgttcgcgg caatcacgtt ggtgctcggc ctggacggtg cggcttccgc
     2461 gcaacaagcg gttgaagtgc tccacaaaac cgtgaaggtc ggcgaccttg atattttcta
     2521 ccgcgaggcc ggcccgaagg atgccccgac cttgctcctc ttgcacggtt ttccgaccag
     2581 ttcgcagatg ttccgcaagc tgatcccgaa gctagcggac aagttccacg tcgtcgcgcc
     2641 ggattacccc ggattcggcc atagctcgat gccgtcgcgc gacgagtatt cttacacgtt
     2701 cgataacctg tcaaagacca tcgacgaatt caccggaaag gtcgggctga cgaaatatgc
     2761 cctctacgtc caggattatg gcggcccggt cggctatcgt ttggctgtgg cacatccgga
     2821 gcggatcacg gccatcgtgg tgcaaaacgg gaacgcatac gacgaggggc tcgacaatga
     2881 cttctggaag ccgctcaaag cgtactggaa ggaaccgaac agccgggaac gacgcgacgc
     2941 ccttcgcgcc ttcctgaagt atgacgcgac caagtggcaa tacactcacg gagtaaagaa
     3001 ccccgaactc gtaagtcccg acggcccggc gcacgaccag ttcctgctcg accgcaaggg
     3061 gaacgacgag atccagctcg acctgttctt gagctacggc agcaacccgc cgctgtatcc
     3121 gaagtggcag gcctacttcc gcgagtacca gcctcccgtg ctgattgtct ggggcaagaa
     3181 tgatcagatc ttcccagccg ccggggccga gccctacaag cgcgacctga cgtcgctcga
     3241 gtaccacctg ctggacgccg gccactttgc cctggaaacc aacggcgacg agattgccag
     3301 gctgatgcgg gaatttctcg gtaagcaagt cgcggcgaaa tgatccgacg gtcgtgagag
     3361 aaaccgtggc cgaccggtcg aagtaatgcg tgcatcaaag tggtttgtgc ccactaacga
     3421 ctggaggagg gagttccatg cgtgcgattc tcagaacggc gttcggcggg ccggagcagt
     3481 tggtcatcag ggagatccca aaacctgagc ccaaagaagg ccacgtggtg atcgcggtca
     3541 aggcgtttgg gatcaatcac gccgagatgc atatgcggaa aggggaatgg gccgagatcg
     3601 ccgatgtaag cggtattgag tgcgtcggcg tcgttgagtc ttgccccggc ggggagttca
     3661 cggtcgggtc caaggtggcc gccttgatgg gcggcctggg ccggacgatc aacggcagtt
     3721 acgccgaatt tacacgcgcg cccgtaacga atgtcgcggc catcgaatcg aacctcccgt
     3781 gggaagacct ggccgcgatc ccggagtcct acgccacggc ctggacatgc ctgttccgca
     3841 acctcgagat cgcttcgggc cagactctgg tgatccgggg cgcgacgtcg tcctttgggc
     3901 aggcagccct caatatggcc gttaactccg gggtacgggt catcgcgacg acccgtaacc
     3961 ggggccggtt ccagacgttg gaggcgctcg gcgcgagtcg agtggaggtg gaggggccgg
     4021 acctctccaa ccgcttgacc gaggcgaagc gggtcgatgc cgtgctcgat ctggtcggca
     4081 acagcacgat cctggactcg ctcgccttgc tccgtcgggg cgggcgcgcg tgcctggctg
     4141 gcttcctcgg cgggctcgct cctgtgcccg acttcaaccc gctcttgcag atggcgagcg
     4201 gggtacattt cagcttcttc ggcagcttcg tgttcggaac gccgggcttc cccttgtcgg
     4261 acgtgccgct ggcggcgatc gctggcgatg tagcggccgg ccggtacaag gcgaagcct
//
LOCUS       DATAQL010000058         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3058942, whole genome shotgun sequence.
ACCESSION   DATAQL010000058 DATAQL010000000
VERSION     DATAQL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3058942"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..877)
                     /locus_tag="VHS97_00930"
     CDS             complement(164..877)
                     /locus_tag="VHS97_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX3446782.1"
                     /translation="MKVLMVITSHDQLGNTGRKTGFWLEELAAPYYTFKDAGVEITLA
                     SPKGGRPPLDPKSNEPEFRTDLTLRFEKDAAAEAQLDKTVRLDSVRQEDFDTVFYPGG
                     HGPMWDLAEDKHSIKLIESFIAAGKTIGIVCHSTGALRHVKAPDGKPLVQGKEVTGFT
                     NGEEEEVGLTKIVPFLVEDEMLKLGATFSKTANWTPHVVSDGQLITGQNPHSSGPAAK
                     ALMAALKQKTKPGATARAS"
     gene            1256..2050
                     /locus_tag="VHS97_00935"
     CDS             1256..2050
                     /locus_tag="VHS97_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3446783.1"
                     /translation="MPSYWRVTFDLPPVNIFGPKETVELGQIITAIELDEQLKVVVFD
                     SAVDGFFITHYDFLAPLEESARIPPGPTGLQALPDMLVRLSRAPVVSIASIRGRATGV
                     GSELALASDMRFASREKAILSQWEVGAGLVPGGGPMARLPRLMGRGRALEVLRSADDI
                     DGDLAERYGYVNRSFPDAELDGFVDALAMRIASFDKQAIADTKRLVDVASLPPDAEIN
                     PEWNAFIAALGRPSSQRRIKALMERGFHRAGDVENRLGFYVGQLNG"
BASE COUNT          413 a          663 c          717 g          477 t
ORIGIN      
        1 ttctcagtct ttgcgtgcat tggagcctcc aagtttgggt tcttgtttga cgccctttga
       61 ccgtctttga cgtagtggag aggcgcctat tgaaagcgcc tctccaacga caatgcgcaa
      121 cctcatccgt gcgagatcag cagtcgcttg gtggatagtc gatctacgaa gcccgagcag
      181 tggcgcccgg cttggtcttt tgcttcagcg ctgccatcag ggctttcgca gccgggcccg
      241 aggaatgcgg gttctgaccg gtgatcagtt ggccgtcgct gacgacgtga ggtgtccagt
      301 tcgctgtttt cgagaaggtg gcgcccagtt tcagcatctc gtcctcgaca aggaagggaa
      361 cgatcttggt gaggccgacc tcttcttctt cgccgttggt gaagccggtc acttccttgc
      421 cctgaaccag cggcttgccg tcaggcgcct tcacgtgacg cagcgcgccg gtggaatggc
      481 agacgatgcc gatggtcttg ccggcggcaa tgaacgactc gatcagcttg atggagtgct
      541 tgtcttcggc cagatcccac atcgggccat ggccgccggg atagaagact gtgtcgaagt
      601 cttcctgcct gacgctgtca aggcggacgg tcttgtcgag ctgggcttcc gcagctgcgt
      661 ccttctcgaa ccggagcgtc aggtcggtcc ggaattcggg ttcattgctc ttgggatcga
      721 gcggcgggcg gccgcccttt ggcgacgcca gcgtgatctc aacgccggcg tccttgaatg
      781 tgtagtaggg cgcagcaagc tcctcgagcc agaagcctgt ctttcgaccg gtgttgccca
      841 gttggtcgtg ggatgtgatg accatcagca ccttcatgtc tgtctcctct gttgcggtca
      901 ctgaccaaag cgccgcctgg caccacgata ggaatgcttc ggcgcgatct cgtcacctcg
      961 caattcatcg ccgagcagct cccgaattgc cgaagcgagt gacgctgctg atagcggacc
     1021 gacggcacgg ctttcactca tacactcagc gccgatcact catacgttga tatgagcgga
     1081 accttcccaa cgttgaatgg tccgggaacg ggccggcgaa cagcatggat tggttcgccg
     1141 tgtagcgacg acgaaccgtg atgccgtcgt gtggcgcgcg tctttcgaga aggagcacat
     1201 cataaatcgc tctgaaagaa ccttcacaac cagggagatc cgtgtcgagc ggcgcttgcc
     1261 gtcatactgg cgggtgacgt tcgatctccc gccggtcaac atcttcggac cgaaagaaac
     1321 cgttgaactg ggccaaatca tcacggcgat cgagctggat gagcagctca aggtcgtggt
     1381 cttcgacagt gccgtcgacg gcttcttcat cacgcattac gacttcttgg cgccgctcga
     1441 ggagtccgca aggattccgc ccggaccgac cggcttgcag gccttgccgg atatgctggt
     1501 tcgtctcagc cgagctccag tcgtgtccat cgcctcgatc agggggcgcg cgacaggagt
     1561 tggcagtgag ctcgcgcttg caagcgacat gcggtttgcg agccgggaaa aggcgatcct
     1621 ctcgcaatgg gaggtcggtg ccggcctcgt gccgggcgga ggaccgatgg cgcggttgcc
     1681 gcggctgatg gggcgaggcc gggcgctgga agtgctgcgc agtgccgacg acatcgacgg
     1741 tgatctcgcg gaacggtatg gctacgtcaa ccgctcgttt ccagatgccg agcttgacgg
     1801 cttcgtcgat gctctcgcca tgcggatcgc atccttcgac aagcaagcaa tcgccgatac
     1861 aaagcgcctg gttgatgtcg cgagcctgcc acccgatgcg gaaatcaacc cggagtggaa
     1921 cgcattcatc gccgcactcg gacgcccatc gagccagagg cggatcaagg cgctgatgga
     1981 acgcggcttc catcgcgccg gcgatgtgga aaaccggctt ggcttttacg ttggccagct
     2041 caacggctga cgaggggagc cagcatcacg gcgtggaccg tttgtccatg tatcgggcgt
     2101 ccagccatct tagcgcggcc gctgcaatga atgccaagct cactcagggc aaaccgagtc
     2161 gcgatccatg tagcggcggc gctcggcaca gtcgattcca tgatcgagtg gcattggatt
     2221 gatctcgagg cgaccatata tggtggcgtg ctcagccgga agtgtggtcg
//
LOCUS       DATAQL010000059         4100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1685312, whole genome shotgun sequence.
ACCESSION   DATAQL010000059 DATAQL010000000
VERSION     DATAQL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685312"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..2141
                     /locus_tag="VHS97_00940"
     CDS             477..2141
                     /locus_tag="VHS97_00940"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX3446784.1"
                     /translation="MGTSTATSGIGCVGRRLFAVLGSSFGGVVAVCLVINLGAPRLIS
                     ADVDGKKQDRTVVAQGRALFEREWQPSDSRTHGGDGLGPVYNDTSCVACHNMGGTGGA
                     GSAGKNVDIITAAFNGQIPTEQPVGVHQERGFLSMAIGSLLGVEQPAKPAAEEKTTVK
                     ATARKPERRKIDTTELVNAHPGFRTSRSVVLHRFGTDESYEQWRQTFVGFQRGNFGRG
                     QAGNGMMLAQLAANADAVVMQNQIGQFFITRSQRNPTALFGAGLIDSIADSDIEAAAK
                     VKYPDFPAIAGRVSRLKDKRIGRFGWKSQTASLADFVLTACAVELGLEVPAHHQGGSP
                     QRPEAAAKGLDLNAQECESLVAYVSDLPRPAERKPESEREAKEIEVGRGLFAKVGCAT
                     CHSPKLGKVENIYSDLLLHDLGPALGDVGQYGVFDPSSSEDEIVDDPGSVAVISESPR
                     RRMIGAGTSPLVAADAQQVKRPTSGPASRFEWRTPALWGLRDSAPYLHDGRAKTLDQA
                     IAFHGGEAGGIAQRFFKLTAKERGQFEAFLKSLTAPAPEELVASAK"
     gene            2276..>4100
                     /locus_tag="VHS97_00945"
     CDS             2276..>4100
                     /locus_tag="VHS97_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446785.1"
                     /translation="MSMRLVPARFRFWRGLAVLAAASAASLIAAQNLRAQESARIASH
                     PSLARYFPSQDLVVYVEFDGLDGHSEAWKKTAAYRLFTETTTGAMYEAALPRLFGLFL
                     ARQNAVRINGSELTEFLSQLFRSGFAVGINRAGGTGPPRSFGLVIRGGAKGDTPQIVE
                     RLLRAGMPIRVAVQDIQKPGGRTVHELGTSPDRSVAWWTEQDDLVVSLISPTGPDAII
                     DALEGRATNAITHPNRQALLRSEDAGGFEPVGLAFFDMAALPPLPSKAVSLGLDRIKR
                     FDYRWGFQGESLLSIVGAVVPAPRTGIPAIFDQPTFDSRHLPPVPNGLGGFTVVSLDG
                     ARVAARIRESLAVLADPPGDNIPAPEQVGEALRAFLGVSLDDEIFAHLGSRFTIYNVA
                     TRINAPSHILESAAQGLFRVPKLAIVAEVKNHDLLAKSIEKLVDHANHTTRGMPMQSM
                     GLNPGEIVRLKNVENGYCWSTLRTRIPIAGDLRPTLLLAQKTLVIASTPAMARKARDL
                     AESPPAGAQPSDNPLTNKLDWLPGNLTLLSVADTAQSVYPELIVGLPRLLESMHGSPM
                     GFPFMMRMNFGPRGGVPDLPGAGPEGPLKALDVELIPDPDEL"
BASE COUNT          783 a         1268 c         1299 g          750 t
ORIGIN      
        1 cttcgtgcag gcacagggcg aggtagtcga atcgcaccaa ctgatggagc cggccggcca
       61 gctcatggaa cagcgcggat aaatcgcggt gcgagacgat cgcttccgag acggccagca
      121 aggctcggta ccgatcgccg gcggaccgcg cctgtgcgtc gaatggagcg cttgccatga
      181 cgaagaccgt gtcagtcata cttccggaac aatcaagtgg tccaccgcac ccccggtgtt
      241 cggagtcgtg tgtgtgagga ggattttacc agttttcagg gcagcgagcg ccgtgtcagg
      301 ctcccgtgca gttgcgcgcg tcggtccgac gacaccgacg cgcgcatgat gccgcacgcg
      361 acgacaatag cgaaagattg aagaaacagg ttgcgacccg acacgcgaca aatgtaatat
      421 gcacgagatc agttcgccct tttcccactt ctcgtcagga atgaaaggga tgcgccatgg
      481 gcacttcgac tgcaacatca gggatcggat gtgtcggccg ccggttgttc gccgttcttg
      541 gctccagctt cggcggggtc gtcgccgttt gccttgtgat caatctgggt gcgccccgtt
      601 tgatctcggc cgacgtcgac ggcaagaaac aagaccggac cgtcgtggcg caggggcgtg
      661 cgctattcga gcgggagtgg cagccgagtg attctcgcac ccacggtgga gacggtctcg
      721 ggccggtata caacgacacc tcttgtgtcg cctgccacaa catgggcgga accggaggcg
      781 ctggctcggc cggtaagaat gtcgacatca tcacggcggc gttcaacggt cagatcccaa
      841 ctgagcaacc ggtcggcgtg catcaagagc gaggcttcct gagcatggcg atcggatcac
      901 tgctcggcgt cgaacaaccg gcaaaacccg ctgccgaaga aaagaccacc gtcaaggcga
      961 ccgcccgcaa gccggaacgg cgcaagatcg acacgaccga actggtgaat gcccaccccg
     1021 gcttccggac gtcgcgcagc gtcgtgttgc accgcttcgg aaccgacgaa tcttacgagc
     1081 agtggcggca gactttcgtc gggtttcaac gcggcaactt cgggcggggt caggctggca
     1141 acggaatgat gctggcacag ctcgccgcca atgccgatgc cgtggtcatg cagaatcaga
     1201 ttggccagtt cttcatcacc cggtcccagc gcaacccgac tgccctcttc ggagcagggc
     1261 tgatcgactc gattgccgat agcgatatcg aggcggcagc aaaggtgaag tatcccgact
     1321 tccccgcgat cgccggccgc gtgagccggt tgaaagacaa gcgaatcggc cgattcggct
     1381 ggaaatctca aacggcttcg cttgcggatt tcgtcctgac agcctgcgcc gtcgagctgg
     1441 gactcgaagt gccggcacac catcaagggg gctcaccgca gcggcccgag gcggccgcca
     1501 agggcctcga cctgaatgcc caggaatgcg agtcacttgt cgcctacgtt tccgatctgc
     1561 cccggccggc cgagcggaag cccgagagcg agcgagaagc gaaggagatt gaagtgggcc
     1621 gcgggctgtt tgcgaaggtc ggctgtgcga cgtgccactc gccgaagctc ggcaaggtcg
     1681 agaacatcta cagcgatctt ttgctccacg acctcggtcc cgcgcttggc gacgtcgggc
     1741 agtacggcgt cttcgacccc agctcctcgg aagatgagat cgttgatgac ccggggtcag
     1801 tcgctgtgat ttctgagtca cctcgccgtc gcatgatcgg cgccggtacg tcgccgcttg
     1861 tcgcggcgga tgcgcagcag gtcaagcgac cgacgagcgg gccggcctcg cgattcgagt
     1921 ggcgcactcc tgctttgtgg ggcttgcgcg attcggctcc ttacctgcat gacggtcgcg
     1981 ccaagacact cgaccaggcg attgccttcc atggcggcga ggcgggcgga atcgctcagc
     2041 gattcttcaa actcaccgcg aaggaacgcg ggcagttcga ggcattcctg aagtctctga
     2101 ccgccccggc cccggaggag ctggttgcca gcgcgaagta agggaagatt cgtccgacgt
     2161 aggtcaggct ttagcctgat gtccctttca tagcttgacc tgagaatcgt caggctgaag
     2221 actgacctac ttacgaatcg tcaggctgaa gcctgaccta cgagaggctc gcgtcatgag
     2281 tatgcgattg gtccctgcgc gtttcagatt ctggcgcgga ttggcggtac tggcagcagc
     2341 gtcggcagcg agcttgatcg ccgcgcagaa tttgcgcgcg caggaatccg cacgcatcgc
     2401 gagccaccct tcactagctc ggtattttcc gagccaggac ctggtcgttt acgtcgagtt
     2461 cgacgggctc gatggccaca gcgaggcttg gaagaagaca gccgcttatc gcctttttac
     2521 cgaaacaacc accggtgcga tgtacgaggc ggcgcttccc aggttgttcg ggctgtttct
     2581 agccagacag aacgccgtac ggatcaatgg gagcgagctg acagaattcc tctcgcaact
     2641 gtttcgctcg ggttttgccg tcgggatcaa ccgagcggga gggaccggtc cgccgcgaag
     2701 ttttggcctg gtcatccgcg ggggggccaa gggtgacacg ccgcagattg tcgaacgcct
     2761 gctgcgtgcg ggaatgccca ttcgagttgc ggtccaagat attcagaagc cgggcggccg
     2821 gaccgttcac gagctcggca cgtcaccgga ccgatccgtc gcatggtgga ccgaacagga
     2881 cgatctggtc gtcagcttga tctcgcccac tggtcccgac gccatcatcg atgcgctcga
     2941 gggccgggcg accaatgcga tcacccatcc caaccgccag gcgctcttgc gcagcgagga
     3001 tgcgggcgga ttcgagcccg tcggactggc tttcttcgat atggccgcac tccccccgct
     3061 gccctccaag gctgtcagct tgggcctcga tcggatcaag cgattcgact atcgatgggg
     3121 gttccagggc gagtcacttc tgagcatcgt gggggcagtc gtgccggccc cacggacggg
     3181 gatcccagcg atcttcgatc agcccacgtt cgactcgcga cacctgcccc ccgtgccgaa
     3241 cgggctcggc ggattcacgg tcgtttcgct ggatggggcc agggtggcag cacggatccg
     3301 tgaatcgctg gccgtgctgg cggacccgcc tggcgacaat attccagcgc ccgagcaggt
     3361 tggcgaggcc ctgcgggcat tcctgggagt ttctctggac gatgaaatat tcgcccatct
     3421 cggatcgcga ttcacgattt ataacgtggc gaccaggatc aacgcgccgt ctcacattct
     3481 cgagagcgcc gcgcagggtc ttttccgcgt ccccaagttg gcgatcgtcg ccgaggtcaa
     3541 gaatcacgac ctactggcga agtcgatcga aaagctcgtc gatcatgcca accacacgac
     3601 gcgcggcatg cccatgcaat cgatggggct caatccaggc gagattgtgc gactcaagaa
     3661 cgtggaaaac ggttattgct ggtcgaccct caggacccga attccgatcg ccggcgactt
     3721 gcgccccacg ctgcttctgg cccagaagac gctcgttatc gcctccactc ccgccatggc
     3781 gcgaaaggcg cgcgatctgg cggagagccc gcccgcgggg gcgcagccgt cagacaatcc
     3841 actgacgaac aagctcgact ggcttcccgg caacttgacg ctgctcagcg tcgccgatac
     3901 ggcgcagtcg gtctatcccg agctcatcgt gggattgccc aggctgctcg aatccatgca
     3961 cggaagcccg atgggcttcc cattcatgat gcggatgaat ttcggtccaa ggggtggcgt
     4021 gccggatctg ccgggagcgg ggccagaggg cccgctcaaa gcactcgacg tcgagctgat
     4081 ccccgacccc gacgagctac
//
LOCUS       DATAQL010000060         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466 k141_6128,
            whole genome shotgun sequence.
ACCESSION   DATAQL010000060 DATAQL010000000
VERSION     DATAQL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6128"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(542..799)
                     /locus_tag="VHS97_00950"
                     /pseudo
     CDS             complement(542..799)
                     /locus_tag="VHS97_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611638.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            945..1163
                     /locus_tag="VHS97_00955"
     CDS             945..1163
                     /locus_tag="VHS97_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446786.1"
                     /translation="MGSASSGSIQSPFLGQRVENVTGEGIWIAKSFLVDRIRNDAFDG
                     RNTFRVPRQMFVEIQSLIARIRAPPVLA"
     gene            complement(1332..>1544)
                     /locus_tag="VHS97_00960"
     CDS             complement(1332..>1544)
                     /locus_tag="VHS97_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446787.1"
                     /translation="RLEFDHHASLPIQGGRSALAFGIEARFIPIFGRAVPPNWPNVMP
                     SPFFRSADGGGRIRRIDEAILKSGTA"
BASE COUNT          285 a          454 c          474 g          331 t
ORIGIN      
        1 ttcgacggtc cctctcggaa ggtgcgaggc gatgtcctgc tcggcctcga tgatcgacgc
       61 cggctaccgt catggcgtcc gcgctgggaa atgatcccga cgctcaggta ctgaaagttc
      121 gcagactcgc tgctggaggg aagtggattc gaacttccgg ttccgcgatg cgcgctcatc
      181 gccaacagcg tgggtcgcgc cgcctgattt cgccggtgag tggcggctcc ttgaatgacc
      241 gccttacaac cccgatcggt tggtgggcca gcaactgctc ggctgacccg ccgcgaggat
      301 cgatcggccc aactcggacg ctgctccgaa aaccttggta atcgtgctgc ggcgaagtat
      361 cggttcctaa acggctcagc cgcatccgcc gtttgtccag accgctcgag ccgagtttcg
      421 aagtctaggt tctccatcaa cgttatgagg atccgtgagc ccgtattgtt tcgtgtccgg
      481 ctttccgatg taaaacttgt tgtcgtggac cgggaaaaag gtcgccatca tccgagccct
      541 gcagcttacg ccgcaggcgc tccaggctgg ccggcaagag ccagttcttg aacggggcgc
      601 cgttacgcaa ggcaccgggc ttcctgacga gcaccggcac gtattgccag ggatcataga
      661 tcgtctcgcc gcggccaaag cggcggcaat gctcgccgac gattgcgcca tcttggcgga
      721 tcacaacgcg atcggcatag gcctggacct cgattggccg accccacacg cgcgaggcaa
      781 ccgaatactt gttgttgtcg ctgattgcct gcggcatgtt gtcgggctcg tcgtctccgt
      841 gctctccggt ttcaaacgac caaccttcga atttccaatc tcgaccgtcg gcgtggcggc
      901 cttgcgggcg catttcgggc gtcagttcat cgaatgcttg atcagtgggt tcggcgtcct
      961 ccggttctat ccaaagccca tttttgggcc aacgcgtcga gaatgtgacc ggcgaaggca
     1021 tctggatcgc caaaagcttt ctggtcgatc gtattcgcaa tgatgcgttt gatggacgga
     1081 ataccttccg agtgccgcga cagatgttcg tcgaaatcca gtcgctgatt gcccggatac
     1141 gggcgccgcc cgtcttagca tgagggtcgc ggaagttggc gatacaggcg caaatggccg
     1201 gggttttcgc gcaacgctgg gcacgaagag cgcgggctgt taccttccaa tctcgatcgt
     1261 gatttcaggg gcgatttggg tgcgaatggg tgcatggaga tccacagcga cggctgtgcg
     1321 taaagacacg attaggctgt accggacttt agtattgcct cgtcgatgcg acggattctc
     1381 ccgccaccat ccgccgaccg gaaaaacggc gatggcatga cgttcggcca attcggcggc
     1441 actgcccgtc caaatatcgg tatgaagcga gcctcgatgc cgaaggcgag ggccgatcgt
     1501 ccaccctgga tcggtaggct ggcgtgatgg tcgaattcca gcct
//
LOCUS       DATAQL010000061         5556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2296009, whole genome shotgun sequence.
ACCESSION   DATAQL010000061 DATAQL010000000
VERSION     DATAQL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2296009"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..2018
                     /locus_tag="VHS97_00965"
     CDS             237..2018
                     /locus_tag="VHS97_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX3446788.1"
                     /translation="MAVLLQIRNAHKSYGGQKLLHGAEASITDDVKVGFVGRNGSGKS
                     TLLKILLGEEELDQGEVLRHPKLRLGYLRQHDAFLADETVLEFLMRDSAQPDWRCGEV
                     AAQFELKGTYLDGPINKLSGGWQTRVKLAALLLHSPNLLLLDEPTNFLDLRTQILLEH
                     FLKNYDRACLIVSHDREFLGATCDHTLELSRGKLAMYPGKIDAYRENQAERKKQDERV
                     NAGVMAKRKHLEEFIAKNKARASTATRAKSKEKQLVKLETVEIAIDEPTVSIRAPQVE
                     PRRGSALRCIDLTIGYPERRIASGIGVEVVHGARTAIVGDNGQGKTTFLRSIVDSLPP
                     LEGEVKWGHGCDIGIYAQHVYTSLPPNQTIYDYLENAAPFGTKTQEILNGAASMLFRG
                     PDVKKKISVLSGGERARLCLAGLLLSEHNVMILDEPGNHLDVESVESLAEALLAYKGT
                     VIFTSHDRHFMKRVATSIVEVRDGRVVNYHGDYEAYLYAVNKEIEEGERETATRMSKA
                     PAEVLKTKAAPKPSLRSERELRKEMSNLEKTIARLDEQKKLSNTQFLSATDSKEAVRL
                     HQEIEDLARQLTDAEERWCVLQEELGE"
     gene            2090..3973
                     /locus_tag="VHS97_00970"
     CDS             2090..3973
                     /locus_tag="VHS97_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEX3446789.1"
                     /translation="MGSCRIAVACLLLLTGRALGQSNTGVDWPAVGNDPGCMRYSTLD
                     QINRSNVTRLKPVWTYHTGELVGRTGKTIECTPIVVDGVMYVTTGYLRVVALHAASGR
                     ELWQFDPRKDHSFPHRLASGGVNRGCAYWSDGKPDGERRIIHGTSDGRLFAIDAKSGK
                     LDSKFGDGGIRNLRKELDPKVAALEYGPTSAPVIWKNTIIVGVSCGEGPGIAAPGDIR
                     AFDVRTGEQVWRFRTVPRPGEFGNDTWKGESWKDRGGANPWGGFSVDVGRGLVFAGLG
                     SAAFDFHGGDRHGDNLFANCTIALDAKTGTRVWHFQTLRHDLWDHDLPTYPNLVKVMR
                     DGKQIDAVVQVTKTGYVFVFDRETGKPLFDIEERPVPPSDIPGEQAARTQPIPVRPPP
                     FSAQFLDETNITNIGEANRASVLAELKKVRSGPAFNPPSRQGTVVIPGFHGGATWSGA
                     SFDPTNGLLYVNSNNVPNIITLIDAKEGSAPRYGSFGHTGYRRFLDHEGYPAIKPPWG
                     VLSAINLNTGEFAWQVPVGEHVELTSRGIPQTGTENFGGTIVTAGGLVFIGGTRDERI
                     HAFDKDTGRLLWEHPLPAGGYATPATFQVNNRQYVVIAAGGAGKLATKAGDAFVAFSL
                     AGE"
     gene            complement(4110..4460)
                     /locus_tag="VHS97_00975"
     CDS             complement(4110..4460)
                     /locus_tag="VHS97_00975"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX3446790.1"
                     /translation="MIRLLADQNFNGRILKGLVRSEPTIDIVHVRDIGLSAETDFGIL
                     ERAAVDGRVLLTHDRQTVPGFAYARVSAGELMPGVFLISKMLPIGQAIDEILLAVQCM
                     SPEECRDIVKYFPM"
     gene            complement(4457..4807)
                     /locus_tag="VHS97_00980"
     CDS             complement(4457..4807)
                     /locus_tag="VHS97_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX3446791.1"
                     /translation="MTILEQKPLTLPVPLREEPQGVLRVGKSRVLLELVIHAYQQGES
                     PAAIVEMYSTLELADVFAVIAYYLTHRAHVDEYLRQCDEKAEAVRRKIESSQRPGPTK
                     EELLARARAKGLIS"
     gene            complement(4846..>5556)
                     /locus_tag="VHS97_00985"
     CDS             complement(4846..>5556)
                     /locus_tag="VHS97_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3446792.1"
                     /translation="RVRDEAADLSRRVSLGDRAKLDEYLTSVREVEKRAGTMRAAKQK
                     ADERASDRGQSAVTMKRPDNGLPEDIREHMRLMCDIVALGFQTDKTRIATLLLCRDIS
                     GLFYPFLDVREAHHLASHSDLSKSYERVTQYYCSQLAYLASRLEAMPEGVGTVLDNSC
                     LLFTSSMWSGTKHDASKLPVVLVGGLGGTLPTGRIFDYTGKGDDNRKLCSMYLSIMDR
                     MGVKLDRFGDADTRLAGF"
BASE COUNT         1122 a         1641 c         1695 g         1098 t
ORIGIN      
        1 ggttcaaggg ccaccgtcgc agccgccgga tcttcaatca ccaccacgtc gagcatcgcc
       61 tcaccgtgcc ttgacaactt ttgttgtcaa gcgtgccctg ccggcttttt tctcgagcaa
      121 attctttcct cttgccgagt ctcaaccaaa aatctccgtt tctcggctta tgcgacgacc
      181 tgtcatctgt tatcatgagc gtctttctaa tctggtccga ggagacccgt ttgacgatgg
      241 ccgttttgct gcagatcagg aatgcccaca agagctacgg tgggcagaag cttttgcacg
      301 gagccgaggc ttcgatcacc gacgatgtga aggtgggatt tgtcggccgg aacgggtcgg
      361 ggaagagtac actactgaag attcttctcg gtgaggaaga gttggaccaa ggggaagtgt
      421 tgcggcatcc caagctgcgg ctgggctatc ttcgtcagca tgatgccttc ctggctgacg
      481 aaaccgttct cgagttcctg atgcgcgaca gcgcacaacc ggactggagg tgcggcgagg
      541 tggcggctca gtttgagttg aagggaacct atctggacgg gcccatcaac aagctgtctg
      601 gagggtggca aacgagggtg aagctggcgg ctctgttgct ccattcgcct aacctgctgt
      661 tgctcgacga gcccaccaac ttcctcgacc tgcgcacaca gattcttctc gagcatttcc
      721 tcaaaaatta cgacagggcc tgcctgattg tctcgcacga ccgggaattt ctcggggcga
      781 cgtgcgacca cacgctcgag ctgtcgcgcg gcaagctcgc gatgtacccc ggcaagatcg
      841 acgcctatcg cgagaaccag gcagaacgca agaagcagga cgagcgtgtg aacgcgggcg
      901 taatggccaa gaggaagcat ctcgaggagt tcattgccaa aaataaggcg cgtgccagca
      961 cggcgacgcg ggcgaagtcc aaggaaaagc agctcgtgaa gctcgagacg gtcgagattg
     1021 ccatcgacga gccgacggtc agcatccgcg cgccgcaggt cgagccgcgc cgggggtcgg
     1081 cactgcgttg tatcgatctg acgattggat atccggaacg gcgaattgct tctgggatcg
     1141 gtgtcgaagt cgtgcacgga gcccgcacgg ccattgtcgg cgacaacggc caggggaaga
     1201 cgacgtttct gcgctcgatc gtcgattcgc tgccaccgct cgagggggaa gtcaagtggg
     1261 ggcacggctg cgatatcggc atctacgccc agcacgtcta caccagcttg cccccgaatc
     1321 agacgatcta cgactatctg gaaaacgccg ctcccttcgg gaccaagacc caggagatct
     1381 tgaacggggc cgcgtcgatg ctgttccgcg ggccggacgt caagaagaag atctccgtgc
     1441 tttccggtgg cgagcgcgcg cggctctgtc tcgcagggct cttgctcagc gagcacaacg
     1501 tcatgatcct tgacgagccg ggtaaccatc tcgacgtcga gagcgtcgag tcgctggccg
     1561 aggccctcct ggcctacaag gggaccgtca tctttacgag ccacgaccgg cacttcatga
     1621 aacgggtggc cacatcaatc gtcgaggttc gcgatggccg ggtcgtcaac tatcatggcg
     1681 attatgaggc ctatctctac gccgtcaaca aggagatcga ggaaggcgaa cgcgagacag
     1741 ccacgcgcat gtcgaaggcg cccgcggagg tcttgaaaac caaggccgcg cccaagcctt
     1801 cgctgcgtag cgaacgggaa cttcgcaagg agatgagcaa cctcgagaag acgatcgccc
     1861 gcttggacga acagaaaaag ctgtcgaata cgcagttcct ctcggccacc gattccaagg
     1921 aagccgttcg gctgcaccag gaaatcgaag atcttgccag gcagctcacc gatgccgagg
     1981 aacgctggtg cgtcttgcag gaagaactgg gagagtaaat cgctcttcgc gagctctccc
     2041 ggtttatact gtggcatcgt ctggtgcggt ccgtctcggg agattggcta tgggttcctg
     2101 tcgtattgcg gtcgcgtgtc tcttactact gaccggtcgc gctttgggac aatcgaatac
     2161 cggcgtcgac tggccggctg tgggcaacga tcccggttgc atgcggtatt ccacactgga
     2221 tcagatcaac cgatcaaatg tcacgcggct gaaaccagtc tggacgtacc acacgggaga
     2281 actcgtaggc cgcacgggca agacgatcga gtgcacgccg atcgtggtcg atggtgtcat
     2341 gtatgtgacg accggctatc tgcgcgtcgt ggccttgcac gcagcgagcg gccgcgagct
     2401 gtggcaattc gacccccgga aggatcattc gtttccgcac cgactggcgt cgggcggtgt
     2461 caaccgaggc tgcgcttact ggtccgacgg caagccggac ggcgagcggc gtatcattca
     2521 cggcacgtcg gacggacgat tgtttgcgat cgatgcaaag agcggcaagc tcgactcaaa
     2581 attcggcgac ggcggtatcc gtaatcttcg caaggaactc gatccgaagg ttgcggcact
     2641 cgaatacggg ccgacatccg ccccagtgat ctggaagaac acgatcattg tcggcgtctc
     2701 ttgcggagaa ggcccaggca tcgccgcgcc gggggatatt cgagcgttcg acgtccgcac
     2761 aggtgagcag gtctggcggt ttcggaccgt acctaggccg ggcgagttcg gcaacgacac
     2821 atggaaaggt gaatcgtgga aggaccgcgg cggcgccaac ccgtggggtg gcttcagcgt
     2881 agatgtgggt cgcggcttgg tattcgccgg gctggggtcg gcggcttttg actttcacgg
     2941 cggcgaccgc catggcgaca atctgttcgc gaattgcaca atcgcgctcg acgccaaaac
     3001 gggcacgcgc gtctggcact ttcagacgtt gcgtcatgac ctgtgggacc acgatttgcc
     3061 gacttatccc aaccttgtca aagtcatgcg cgacggcaaa caaatcgatg ccgtcgtgca
     3121 ggtgacgaaa acgggctacg tgttcgtgtt cgaccgcgag accggcaagc ccctcttcga
     3181 catcgaggaa cgcccggttc ctccatcgga catccctggc gagcaggcgg ctcggacgca
     3241 gccgattcct gtcaggccgc cgccgttctc cgcccagttc ctcgacgaga cgaacatcac
     3301 gaacatcggc gaggccaacc gtgcgtctgt tcttgccgag ctgaaaaaag ttcggagcgg
     3361 gccagccttc aacccgccca gccggcaagg gaccgtcgtt attcccggct tccatggcgg
     3421 agcgacctgg tccggggcat cgtttgatcc cacaaacggc ctgttgtatg tcaactcgaa
     3481 caatgtcccc aatatcatca cacttattga tgcgaaggag ggcagcgctc cgcgttatgg
     3541 atcttttgga catacgggct accgccggtt ccttgaccac gaaggatatc cggcgatcaa
     3601 gccgccatgg ggggtcttga gtgcgatcaa cctcaacacg ggcgagtttg cctggcaggt
     3661 cccagtcggc gaacatgtgg agctgacgtc gcgcgggatt ccgcaaacag gcaccgaaaa
     3721 ctttggcggc acgatcgtga cggccggcgg cctggtcttt atcggtggga ctcgggacga
     3781 gcggattcac gcgttcgaca aagacacagg ccggcttctt tgggaacacc ccctcccggc
     3841 cggcggctac gcgacccccg cgacttttca ggtcaacaac cgccaatatg tagtgatcgc
     3901 agccggcggg gctggcaaac ttgccactaa ggcgggcgat gcttttgtgg cgttttcgct
     3961 ggcaggcgag taatgtgcgc cgggctctct ctcagcaggc gggggtaacc tataccgctc
     4021 ctcgatgtgc actttctcaa tctcggcctt cggaggatgt ccacccagac gagtagccga
     4081 cgatcggcgt accgctttag cccactgatt cacatcggga aatacttgac aatatccctg
     4141 cattcctcgg gggacataca ttgaaccgcc aaaaggatct catcgattgc ctgaccgatg
     4201 ggaagcattt tactgatgag gaaaaccccc ggcatcagtt cccctgccga gactctagca
     4261 taagcaaacc cgggcaccgt ttggcgatca tgggttaaca gcacccgtcc atcgacagcc
     4321 gcccgttcga gaattccgaa atcggtctct gccgacaagc cgatgtcgcg tacgtgaact
     4381 atgtcaattg tcggctctga acgcactaag cccttcagga tgcgtccgtt gaaattctga
     4441 tccgccagga gtcgaatcac gaaatcaacc ctttggccct ggcgcgggcc aacagttcct
     4501 ccttggtggg cccaggtcgc tgggagctct cgatcttgcg gcgtacggct tccgcttttt
     4561 catcgcactg gcgaaggtat tcatcgacgt gagcgcgatg cgtcagatag tacgcaataa
     4621 ccgcaaagac atcggctaat tccaaggtgc tgtacatctc gacaatagcc gcgggcgatt
     4681 cgccttgctg gtaggcatga attacgagtt ccagcaggac ccggctctta cccacgcgta
     4741 agacaccctg cggttcttcg cgcaagggga ccggtagcgt cagtggcttc tgttccaata
     4801 tggtcatggt cccgttctcc ctcggatcac ggtgtcttta tccgctcaaa aaccagcgag
     4861 cctcgtgtct gcatcgccga agcggtcgag cttgacaccc atgcgatcca tgatcgagag
     4921 gtacatggag cagagtttgc ggttgtcatc ccctttgccg gtgtagtcga aaatgcggcc
     4981 cgtcggcagc gtcccgccca ggccgccgac caggacgacc ggcaacttgc tggcatcgtg
     5041 cttggtgccg gaccacatgc tcgaggtgaa gagcaggcac gagttgtcga gcacggtgcc
     5101 caccccttcc ggcatggcct cgaggcgcga ggcaaggtac gcaagctggc tgcagtagta
     5161 ctgagtgacg cgctcatagg atttagacag gtcgctgtgc gaggccaggt ggtgcgcctc
     5221 gcgcacgtcg aggaacgggt aaaacagccc ggaaatatca cggcagagca agagcgtggc
     5281 aatacgcgtc ttgtctgtct ggaagccgag ggcgacgatg tcgcacatca gtcgcatgtg
     5341 ctcgcggatg tcttcgggca ggccgttgtc cgggcgcttc atcgtgactg cagactgccc
     5401 gcggtcgctg gctcgctcgt cggccttctg tttggcggcc cgcatcgtgc cggcgcgttt
     5461 ctcgacctcg cggacgctcg tgaggtactc gtcgagcttg gcccggtcgc ccaagctcac
     5521 ccggcggctc aagtccgcgg cctcgtcgcg cacgcg
//
LOCUS       DATAQL010000062         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5349392, whole genome shotgun sequence.
ACCESSION   DATAQL010000062 DATAQL010000000
VERSION     DATAQL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5349392"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VHS97_00990"
     CDS             complement(<1..189)
                     /locus_tag="VHS97_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446793.1"
                     /translation="MCVPFPAATGFILDTDFYDIKLTTRGKGVPCRSLTNSKSLLTSV
                     LNQACDQTEFERLSRRKIP"
     gene            188..1366
                     /locus_tag="VHS97_00995"
     CDS             188..1366
                     /locus_tag="VHS97_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446794.1"
                     /translation="MKIFWSWQADTRGKTGRHFVREALADAIKALKQPEKVEEPSERE
                     TREALHLDHDRQGVPGSPDLAPTIFRKIDQAAVFVADVTLVAQMEGEDGSAKKKLINS
                     NVAIEYGYAVHALGDESILMIQNVHYGEREELPFDLKHKAGPVQYQLAPDATKQEIAA
                     ERARLRAKFVEALRPYLSIGKAPGEPARKFEETPCTANAAFFWTPSDVLARIGSDTPP
                     AFRHKSEDDDAIEYRFNEPRAFYLRLIPTIPLAEELKVTTLLDVVQRRPLEVLTRTVF
                     GGGMSGRNRFGAISYEPHGISPVPTGFTQLFRNGEIWGVSGQFAIRYYDELVVPMVNV
                     DNIYKRVLANYISVAGEDLGVTRPYQVEMGAVGLKDMRLCLPQPVSAGDEVSGPIYEN
                     "
     gene            1661..>2055
                     /locus_tag="VHS97_01000"
     CDS             1661..>2055
                     /locus_tag="VHS97_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446795.1"
                     /translation="MSEALLRDIAEYCRGAGIAESTFGRLAVNDGKLVGRLRLGGRVT
                     TDTVERVRAFIAREPHPAVNGVPVDLPVSLSPPTAAAAHNFRFYDNRQKYLLFVTTCS
                     EKQVIAQRVGLELANIHPRPPALRVFDAG"
BASE COUNT          420 a          606 c          632 g          397 t
ORIGIN      
        1 tggtatcttt cggcggctca ggcgctcgaa ctcggtctgg tcgcaggctt ggtttaaaac
       61 cgacgtcaac agtgatttcg aattggttaa cgagcggcat gggacccctt ttccccgggt
      121 ggtcaactta atatcgtaaa aatctgtatc aagaataaaa cctgtggccg caggaaatgg
      181 gacacacatg aagatcttct ggtcatggca ggcggatacc cgaggtaaga caggccgcca
      241 ttttgtgcgc gaggctctcg ccgacgcgat caaggcactc aagcagccag agaaagtcga
      301 ggagccgtca gagcgcgaga cgcgcgaggc ccttcatctt gatcacgacc ggcagggggt
      361 gcccggcagt ccagaccttg ccccgacaat tttcaggaag atcgaccagg cggcggtgtt
      421 cgtggcggac gtcacgctcg ttgcccaaat ggaaggggaa gacggcagcg ccaaaaagaa
      481 actaatcaat tcgaatgtcg cgatcgagta cgggtacgcc gtgcacgccc ttggagacga
      541 atccatcctg atgatccaga acgtccacta cggcgagcga gaggaactgc cgttcgatct
      601 gaagcacaaa gcaggcccgg tccagtatca attggcgcct gatgcgacca aacaggaaat
      661 cgccgcggag cgcgcgaggc tgcgggccaa attcgtcgag gcgctcaggc cgtacctgag
      721 tatcggcaag gcgccgggcg aacctgcccg caagtttgag gaaacgccct gcacagcaaa
      781 tgcggccttc ttctggaccc cgtcggatgt gttggccagg attggctcag acacgccccc
      841 tgcttttcgg cacaaaagcg aagacgatga cgcaatcgag tatcgcttca acgagccgcg
      901 cgcattctat ttacgtctga tccccacgat cccactggct gaagagctaa aggtgacgac
      961 gctcctcgac gtcgtgcagc gccggccgct tgaggttctc actcgtaccg tgtttggcgg
     1021 aggaatgtcc gggcgtaacc gcttcggcgc aatcagttat gaaccgcacg gcatctcgcc
     1081 ggtgccgact ggctttaccc agcttttccg caacggcgag atttggggtg taagtggcca
     1141 gtttgccatt cgctattacg atgagctcgt tgtgccgatg gtcaacgttg ataatatcta
     1201 taagcgcgtg ctggcgaact acatctcggt agccggtgaa gatctggggg tcacccgccc
     1261 ctaccaggtc gagatggggg cggtcggact aaaggacatg cgtctctgtc tcccgcagcc
     1321 ggtaagtgcg ggtgacgagg tgtcagggcc gatttatgag aactaactca agctccggcg
     1381 agtattaaac gacacgggcc cggggaatca gcgggcgctg atcgaagagt ttctcgacgc
     1441 gctctacgac ctcgcgggag tgacgagatg acggtccagg ggcaaacccg acttagttaa
     1501 ctctcccggg gccgctggtg cgggtctcgg cccacggccg ctcaaattct tcatgggatg
     1561 tagcccacta tcttgcccac cctgatagtg ggcattgttt tagttgacct ggagctgacc
     1621 ccggagctta atcccggtgc gggtaccggg aggtcgtccg atgagcgagg cgctgctgcg
     1681 cgacatcgcc gaatactgtc gcggcgcggg cattgcggaa tcgacgttcg ggcgcctcgc
     1741 ggtgaacgac ggcaagctgg tcggccgact gcgcctcggc ggccgcgtca cgaccgacac
     1801 cgtcgaacgc gtgcgggctt tcatcgcccg ggagccgcat cccgctgtca acggtgtgcc
     1861 ggtcgatctg ccggtgtcgc tgtcgccgcc cacggcggcg gctgcccaca atttccgctt
     1921 ttacgacaac cgccaaaaat acctgttgtt cgtcaccacc tgcagcgaga agcaggtgat
     1981 cgcccagcgc gtcggcctgg agctcgctaa catccatccg cgtccgcccg cgttgcgcgt
     2041 cttcgacgcc ggcat
//
LOCUS       DATAQL010000063         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_616664, whole genome shotgun sequence.
ACCESSION   DATAQL010000063 DATAQL010000000
VERSION     DATAQL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616664"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VHS97_01005"
     CDS             <1..347
                     /locus_tag="VHS97_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3446796.1"
                     /translation="RIVNIGTSLLAATTGFYSAYAGSKAPLEHFTRALAKEVGSRGIT
                     VNTVAPGPLNTSFFYPAETDAATEFHKSMSINGKLGEVKDIVPLVKLLVSPEGCWITA
                     QTIFINGGYAAR"
     gene            complement(452..685)
                     /locus_tag="VHS97_01010"
     CDS             complement(452..685)
                     /locus_tag="VHS97_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446797.1"
                     /translation="MPEYFTNIDVSGPVLHGELPSMIGVVESPQSLEPIGTAALIGST
                     DKGLAEWSLRVRGANVPGHWVIIGNWFVPDEGW"
     gene            complement(926..1678)
                     /locus_tag="VHS97_01015"
     CDS             complement(926..1678)
                     /locus_tag="VHS97_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446798.1"
                     /translation="MRNLHGTSSSAGADVRRALAQYHSTLQQVRELNNAEPEPDEPIE
                     VQQGFERLAAATARAEQMARDALSNAVASFAGDVPCSVIVETRGGSSVVTLAGKPNGL
                     IVAAAADRVPVKDIVCRPEPPPEFAHEFRQMAPNQGGVYLVLQGDDVVAVYNGVYFRT
                     TNEVVADFLELIQAGFEGVWSDDYVVWCLGRIVAVIHQPMDGKEQKVFLFNEERNDPI
                     AGRVIEPMPYWPTYQEWVAGGRGDLWKTERYP"
     gene            1919..>2121
                     /locus_tag="VHS97_01020"
     CDS             1919..>2121
                     /locus_tag="VHS97_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446799.1"
                     /translation="MPGINNRSIIPLQFSHNSQFKCQALRVCEAAFEFYEKCLGGRIV
                     MMLTYGDSPMADQTPPDGHEKIL"
BASE COUNT          430 a          649 c          620 g          422 t
ORIGIN      
        1 gccggatcgt caacatcggc acttctctct tggccgcaac gactggcttt tattctgcct
       61 acgcgggaag caaggccccc ctcgaacact tcactcgcgc tctcgccaaa gaggtcggaa
      121 gtcgcggcat caccgtcaat acggtcgcgc caggaccctt gaacacctca tttttctatc
      181 ctgccgaaac cgacgcggcc accgaattcc acaagagcat gagcatcaac ggcaaactcg
      241 gcgaagttaa ggatatcgtg cctttggtga agctattggt gtccccggag ggatgctgga
      301 tcactgccca gaccattttc atcaacggag gatacgcggc ccgctaattc gggagcgatc
      361 cggggatcct cgccacgcca atcgcttcga tctatcacgg ctgtgtgttg tagaccacgt
      421 tcacggtcac ttggagcgcc ccggtgcgtg gttaccaacc ttcgtcgggg acgaaccagt
      481 tgccgatgat gacccagtgg cccggcacgt tggcgccgcg gacgcggagc gaccactccg
      541 ccagcccctt gtcggtcgat ccgatgagag cagccgtgcc gatgggctcg aggctttgcg
      601 gcgattcgac aactccgatc atactgggga gctcgccgtg gagaacgggc ccggagacat
      661 cgatgttggt aaagtattcg ggcatgatgt caccgcctga tcctgcgctg ggcgctcgga
      721 tcaattgccg tgctgacaaa atttgacaag gccgacggtt cgccgctgga acgaaaccgt
      781 ggccacagtg tcaggatcga cggattggag ctttgtggcg gctccggcgg acaagagcgc
      841 cgctagcttc ggcatcatgc agaatcgttt tcccgtcgcg tcctatgcac ttccaacata
      901 attatagctc atgctgtgcc agtcttcatg gataccgttc ggttttccag agatcacctc
      961 ggccgccggc gacccattcc tggtatgtgg gccagtacgg catgggttcg atcacacgac
     1021 ctgcgattgg gtcgttgcgc tcttcgttga agaggaagac tttctgctct ttcccgtcca
     1081 tcggttggtg gatcacagcg acgatacggc caaggcacca gaccacgtaa tcgtcgctcc
     1141 agaccccctc gaatccggcc tggatcaact cgaggaagtc ggcgacgact tcgttcgtgg
     1201 tgcgaaaata aacgccgtta tagacggcga cgacatcatc cccttgcagc acgagataga
     1261 cgcccccttg gtttggggcc atctggcgaa actcgtgggc aaactcgggc ggtggctcag
     1321 ggcggcagac gatgtccttg accggcacgc gatcggctgc ggcggcgaca ataagcccgt
     1381 tgggcttacc cgcgagggtg actaccgagc tccccccgcg ggtttcgacg attaccgagc
     1441 aaggaacatc gcccgcgaat gacgccacgg cgttggacag cgcgtcgcga gccatctgtt
     1501 cggcgcgggc cgtcgccgcg gccagccgct cgaacccctg ctggacctcg atgggctcgt
     1561 ctggttcggg ctcggcgttg ttgagctccc gaacctgctg caaggtggaa tgatattgcg
     1621 ccagtgcgcg gcgaacgtcg gcaccggctg aactgctcgt tccatgtagg ttccgcatga
     1681 tcgtcctaac gctccatcgg ccggactatc cttcgacttc cggcctctca tctccatctc
     1741 gagtggggcg cgaacgccgc acaaggtact tgataatatc gcagcggaac caagaggcaa
     1801 tgatctggtc cggcagactg aaaacggaat caggaaccgg cggcgaaaca ccgccaggtt
     1861 caatggcagt cgcgaggcgg agcattctca cccagattgc ccacagtggg catactagat
     1921 gccaggcatc aacaatcggt caataatccc cctccagttc tcccataaca gccagtttaa
     1981 gtgccaggcg ctaagggtat gcgaggcggc cttcgagttt tacgagaagt gcctgggcgg
     2041 ccgaattgtc atgatgttga catacggcga ttcgccgatg gccgaccaaa cgccccccga
     2101 cggacacgag aagatcctcc a
//
LOCUS       DATAQL010000064         6523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_769118, whole genome shotgun sequence.
ACCESSION   DATAQL010000064 DATAQL010000000
VERSION     DATAQL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769118"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VHS97_01025"
     CDS             <1..1648
                     /locus_tag="VHS97_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX3446800.1"
                     /translation="GAADRGGLAVGDYFGNGRQDIAVAIFGNNTVAILPNNGDGTFGT
                     PAALAMPSGFTNIRSVTTANFFGNGFADLAVAGGEGYNNVLSSTNPAGVALFENDGKG
                     HFTFEGKYLAAVTPDPGGGNGQGDTINPEHVNAADLNNDGKPDLVLSLYDHNIDVFIN
                     NGNGTFQPAVPYTTETPGAVGGYPRGVVFGDFNGDGIVDIATLNVGQPVPADQSTPEP
                     GSVGVLYGNGDGTFQAPIQYTPYDLPGGLAVGDFNSDGLADLAVTQNYDGHSVSVMLN
                     QPNTANQPPTVTGVSPASGATSGGTVVTIAGTNFTGTSYVDFGSVSALSFTVNSDTSI
                     TATAPAEATGTVDVTVYNSGTSTTNAADSFTFTTVSTTQVDLSSAFNRTGIVADGTNF
                     GGGGLDGGGNAFSSNLLGTSLTAGGTTFGFGPAGASDVVSAAGQGIALPACNDSALEL
                     LATGVNGNQANQTFTVTYTDGTTATFTQSISDWYTAQGYAGESTALSTAYRDTSGGTE
                     QAGPFNVYEYTLPLDPTKAVSSLTLPEDGNALVLAIDVLP"
     gene            complement(2479..3123)
                     /locus_tag="VHS97_01030"
     CDS             complement(2479..3123)
                     /locus_tag="VHS97_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX3446801.1"
                     /translation="MNRSLASGSFGDPATDQIVKLDGTPGTIAHRHCKRLRLVRAITN
                     SAGNRGGSLPPHCRSGIRGGGAALTEMLAALTGVFPAAGAIVQHLPPRSPSVLAHVLS
                     RHSAIPVTDTKDGELIRQARAYLAPADYHLLVNADRTFSRTQSDKVHGTRPAAENLFE
                     SAAKSLNERIIAVVLTGADGDGESGVQTVKQMGHAGACRAMNVNVCLIQDLLTF"
     gene            complement(3278..4186)
                     /locus_tag="VHS97_01035"
     CDS             complement(3278..4186)
                     /locus_tag="VHS97_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX3446802.1"
                     /translation="MPIQEPRSTIDDSTAEHQFIADSRPPRVAIKFGAMTDCGKVRSN
                     NEDHFLVARLSKSMRVCKTSLPGEGETQFSEEEGYLMVVADGMGGAEAGERASALAVG
                     SIEGFVLNTLKWFLHLSGPDEHQLVTELRQSLESADRTVIERARSNRAFYGMGTTLTM
                     AFSVATDLFIVHAGDSRAYLFRDGELEQLTSDDTVVQVLVDAGVISPEDAKHHNRRHV
                     VTNVIGGPGEGVSVEIQKLKLLDGDTVLYCTDGLTEPVDDHAIAEILHHHPDPEDACT
                     RLVELALSRGAPDNVTAVVVRYQVDR"
     gene            4412..4744
                     /locus_tag="VHS97_01040"
     CDS             4412..4744
                     /locus_tag="VHS97_01040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX3446803.1"
                     /translation="MRATWDFDHPRGTTTSLYSDGVPEAMEPAGERFGDARLLAAIGR
                     GRSEPLQAGVLLTLGALAAFAGCESKGPAEKAGESIDKGVQNAKDVVNPPGAGEKAGR
                     EVDKALKP"
     gene            4961..6202
                     /locus_tag="VHS97_01045"
     CDS             4961..6202
                     /locus_tag="VHS97_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3446804.1"
                     /translation="MGWVLRGLGHVVRAGIGKGFLLWERSFAWASWQEFLVIVCGVFL
                     LGGVAGGRLPGLRVVCGLATLGMGVVACLAYMFIDLERNEVERGHKAVHNPLKGQILA
                     RNLERYGKQVGIPLLIVAAIALIGGFAFLNQGLYETIGKHWYAIADERRLPIYVDFLA
                     YTLTKILGLIDVLDLARSHHILGTAFVRPAAWPANALLAGLKVFFTFVLLHQIFASLR
                     QGKLLAETVADFWSPHEPIHDRACGALPIYGIVAIGPLLRSLRLVTSLTKEQRTEIPL
                     ILETIGPSIIPALVRHLRDPHEDVRAIAAAALGHLHAVETVPALAALALDPSDSMRQS
                     VVAAIGSLGKSSTGSARTRRGLAGRSGFLGRAIWRTVWWKKRPALAPPGDPIELAVAT
                     CFEKGGRALSADARPALAGPG"
     gene            6165..>6523
                     /locus_tag="VHS97_01050"
     CDS             6165..>6523
                     /locus_tag="VHS97_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3446805.1"
                     /translation="MPTLVPLLQDRDESVRTAAAEAIAQVGTLNHAATAILSLGLDSR
                     DNVVRAQTAEALGTIGAAAEDAAPALVQATIDGNDRVRAKAVQALGKIGETAAVVAVP
                     GLVRALSDRDNWVSALAA"
BASE COUNT         1203 a         2032 c         2064 g         1224 t
ORIGIN      
        1 cggcgcggca gaccggggcg ggttggcggt gggcgattac ttcggcaacg gccggcaaga
       61 tatcgcggtg gcgatcttcg gcaacaacac tgtggctatc ctgcccaaca acggtgacgg
      121 caccttcggg accccggcgg cgctcgcaat gccctccggg ttcacgaaca ttcgctccgt
      181 gaccaccgcg aacttcttcg gcaatggctt cgcggacctg gcagtcgccg gcggggaagg
      241 ttacaacaac gtcctttcaa gcaccaatcc cgccggtgtc gccctcttcg agaacgacgg
      301 caagggccat ttcactttcg agggaaagta tctcgccgcg gtaacaccgg atcccggcgg
      361 aggcaacggg caaggcgaca caatcaatcc agagcatgtc aacgccgccg acctcaataa
      421 cgatgggaag cctgacctgg tcttatcgct ctacgaccac aacatcgacg tgttcatcaa
      481 taacgggaac ggcacctttc aaccggccgt cccttacacg accgagacac caggggcggt
      541 cggcgggtat ccgcgcggcg tggtcttcgg cgacttcaac ggcgacggta tcgtcgacat
      601 tgcgactctc aacgtcggcc agcccgtccc tgcggaccag tcgacacctg agcccggctc
      661 ggtaggggtc ttgtacggga atggcgacgg tacgtttcag gccccaattc aatacactcc
      721 gtacgaccta cctggtgggc ttgcggtcgg tgacttcaac agtgacgggt tggctgatct
      781 cgccgtcacg caaaattatg acggccactc ggtctcggta atgctaaatc aacccaacac
      841 ggccaatcaa ccccccacgg tcacgggtgt cagtcccgcc tccggcgcta ccagcggcgg
      901 caccgtggta accatcgccg gaacaaactt caccggcact tcgtacgtcg atttcggcag
      961 cgtgagcgct ctctcgttca ccgtcaactc cgatacgtcg atcacggcga cggcaccagc
     1021 ggaggcgacc ggcacggtgg acgtcacggt ctacaactcc ggtacctcga ccacaaacgc
     1081 ggccgacagc ttcacgttca ccacggtgag cacgacgcaa gtcgatctct cctccgcctt
     1141 caaccggacg gggatcgtgg ccgacgggac gaatttcggc gggggcgggc tcgacggcgg
     1201 cggcaacgcc ttctcgtcca acctgctcgg gacgagcctg acggccggcg gcaccacctt
     1261 cggctttggc cccgccgggg ccagcgacgt cgtcagcgcc gccgggcagg gcatcgccct
     1321 gccggcgtgc aacgacagcg cgctcgagct actggcgacc ggcgtcaacg gcaaccaggc
     1381 gaaccagacc ttcaccgtga cctacaccga tgggacgacg gcgacgttca cgcagtcgat
     1441 cagcgactgg tacacagcgc aggggtacgc cggggagtcg acggccctgt cgaccgccta
     1501 tcgcgacacg tcgggcggga cggaacaggc cgggccgttc aacgtctacg aatacacgtt
     1561 gccgctcgac ccgaccaagg cggttagcag cctgaccctg cccgaggacg gcaacgccct
     1621 ggtgctagcg atcgacgtgc tgccgtaacc gacctcatcg gtgagtggcc gggtggtcgg
     1681 ggtggcggat tggccgcccc gatccccagc cagttgtagg ccgcggacga tgcggttgat
     1741 gaacgccgtc gtgggctggg ccaccgcaga gtcgcccgcc gcaggctgcg gtgctgacta
     1801 cgacagtagg actatcggcc actatcactt ggcagctggg cggaacaatc ccttgacgtt
     1861 tcgggccagc accatttttt cccggccctt cctctgcacc cggtaggtag gcagaatctg
     1921 gcctgtggca gccaaatgct tcatttcaac ggaggtgcag ggtccgagca tcctttggcc
     1981 atccgacgag tacaaccact ggacaggcat tgaaagcctc ctacgaagag agaacggcac
     2041 tgcataaacg cacgatgacg gaaggactga tgaggctgtt ttggggaagg cgccaccgtt
     2101 ttgaaacttg acgcagtcct acggtgccaa ccggagaatg aggatacgac tcgacgggcg
     2161 ttggatgccc taatacatga acgggatggg aaaaagcttc cgcgttccaa ccaccttaat
     2221 tggttcgttt ccatcttctc cctgacgact tcgcaccttt cgcgcaccgc aatcagtaaa
     2281 gctatttgct tgcgttgctg tctctggtca gtcgtcatgg cactctgcct gcgataagaa
     2341 tcaccatcag ccaacgcagt cgcgttgcgg cggccgttac tggctgagta ggcatgacgt
     2401 cggttcccgg taggttggaa ccatgccgca tcacgctctg tagctcatta ttctaccgac
     2461 attccgagat ctgtacaatt agaatgtcaa aagatcttga atcagacaaa cattaacgtt
     2521 catggctcgg cacgcacccg catgccccat ttgtttcacg gtctgcacgc cgctttcgcc
     2581 gtcgccatca gctccggtca ggacaaccgc gatgattcgc tcgttcaaac tcttcgccgc
     2641 cgactcgaag aggttttcgg cggcgggccg ggtgccatgc actttatccg actgcgtgcg
     2701 cgaaaaagtg cgatcggcat tgaccagcag gtgataatcg gccggtgcga gataggcccg
     2761 ggcctggcgg attagctcgc cgtcctttgt atcggtgacc ggtatggcgg agtggcggct
     2821 gaggacgtgg gcgaggacgc ttggtgaccg cggtggcagg tgctgaacaa tggcgccggc
     2881 cgcgggaaag acgccggtca gggcggcgag catttcggtc agggccgcac cgcccccgcg
     2941 gatgccgctc cgacaatgag gcggaaggga tcctccgcga ttgcctgcgg aattcgtgat
     3001 ggctcggacg agccgaagac gtttgcaatg ccgatgggcg atcgtgcctg gcgttccatc
     3061 cagcttgacg atttgatcgg tggcggggtc accaaagctg ccggaagcga ggctgcgatt
     3121 cacgtggagg cgttcggcgt gaggatcatg agggtacggt gaggttggag tctgagataa
     3181 tcgaccgcaa gtttgagtaa ggcgccggtg gcgtcacgga acgggaaaat ttccccacaa
     3241 tgaatacgaa aatacgctcg cagaactttt ttgtattcta tctatcgacc tgataccgga
     3301 cgacaactgc agtcacattg tccggcgcgc ctcggctaag ggccaactcc accaaccgag
     3361 tgcacgcatc ctcggggtcg ggatgatgat ggaggatctc ggcgatcgcg tggtcgtcga
     3421 ccggctcggt caggccgtcg gtgcagtaaa gcacggtgtc gccgtcgagc aatttcagct
     3481 tctggatctc gacagagacg ccctcgccgg ggccgccaat gacgttggta acaacgtggc
     3541 gccgattgtg gtgcttggcg tcctcgggcg agatcacgcc ggcgtcgacc agtacctgga
     3601 cgaccgtatc gtcgctggtc aactgctcga gctcgccatc gcggaacagg taagcgcgcg
     3661 agtcgccagc gtgtactata aaaaggtcgg tggcgacgct aaaggccatc gtcagggtag
     3721 tccccatccc ataaaatgcg cgattgctgc gggcgcgctc gatgacggtc cgatcggcgg
     3781 attcgaggct ctggcgaagc tcggtcacga gctggtgttc gtcggggccg ctcagatgca
     3841 agaaccattt gagcgtgttc aagacaaatc cctcgatgct gccgaccgcc aaagcgctgg
     3901 cgcgttcgcc ggcctcggcg ccgcccatgc cgtcggcgac gaccatcagg tagccctcct
     3961 cttcggagaa ctgcgtctcg ccttcgccgg gcaggctggt cttacacacg cgcatcgact
     4021 tggagagcct ggcaaccagg aaatgatcct cattgttgct gcggaccttg ccgcagtcgg
     4081 tcatggcacc gaacttgatc gccactcgag gcgggcggga atcggcgatg aactggtgct
     4141 cagcggtcga gtcgtcgatc gtggagcgag gctcttggat aggcatgatc gcagtcctct
     4201 gcctgacgca ctggttctag cgaagggcgc ttcacgcaca tctcgtgccg gatttcccgc
     4261 gtctcttctc cagtcggcaa gtggtaccca tcgacccgga ctggtcatcc atccgggagt
     4321 ttcgccggcc gatccgccgt gtgccgaccg agctggttgt gacagttggt aaacgcccct
     4381 gctcgccgga cgagaccaac cgcctcatta tatccgcgca acatgggatt ttgatcatcc
     4441 tcgaggcacc acgacaagcc tttactccga cggcgtcccc gaggcgatgg agcccgccgg
     4501 ggagcgcttt ggcgatgcgc gtctcctggc ggccatcggc cgagggcgat ccgaaccact
     4561 ccaggcgggt gtgttactga cgttgggggc gctggcggcc ttcgccggtt gcgagtccaa
     4621 ggggcctgcc gagaaggccg gcgagtccat cgacaagggc gtccagaacg caaaggacgt
     4681 ggtcaatccg ccaggcgccg gggaaaaggc ggggcgggag gtcgacaagg ccctcaaacc
     4741 ctgaacggca gccgaaaacg agaggcgatc cggcctgttc tttctctccc cggtaccgga
     4801 tcgtctccgt ttccattgga gcgatgcgga tcgcgaggcc acgttgatgc atacgtcctc
     4861 ctggttcgtc aggctggaac aaacgcaaac atcgtatctg ctcctggcga tactggcggt
     4921 gaccgctgcg gcggccgcga tcctctaaca ggtcggggtc atgggctggg tcttgcgtgg
     4981 cttgggccat gtggtccggg cagggatcgg caaaggcttc ctgctttggg agcgctcgtt
     5041 cgcgtgggct tcgtggcagg aattcctggt catcgtctgc ggagtgttcc tcctgggcgg
     5101 ggtggccggc ggtcgattgc ctggattaag ggtcgtttgc ggcctcgcga cactgggaat
     5161 gggagttgtt gcgtgcctcg cctacatgtt catcgacctg gaacgaaatg aagtcgagcg
     5221 gggccacaag gccgtccata atccgttaaa aggccagata ctggcgcgaa atctcgagcg
     5281 gtacggcaag caggtcggca tccccctctt gatcgtcgcc gccatcgcct tgatcggcgg
     5341 atttgctttc cttaaccagg gcctctatga gacgatcggc aagcactggt atgcgatcgc
     5401 cgacgagcgc aggctgccga tctacgtcga tttcctcgca tacaccctca cgaaaatcct
     5461 gggcctgatc gacgtgctgg acctggccag gtcgcaccac atcttgggca ctgcgttcgt
     5521 tcgtcccgcc gcgtggccgg ccaatgcact tttggccggg ctcaaggtgt tcttcacatt
     5581 cgtattgctc catcagatct ttgcctcgct gcggcagggc aaactgctcg cggagacggt
     5641 cgctgatttt tggagcccgc acgagccgat ccacgaccgt gcctgtggtg cgctgccgat
     5701 ctacgggatc gtggccatcg gaccgctttt gaggtctctg cgcctggtta catcgctgac
     5761 caaggagcaa cggaccgaga tcccgttgat cctggaaacg atcggcccgt cgatcatacc
     5821 ggccttggtc cgtcatctcc gcgaccctca cgaagatgtg cgggcgatcg ccgccgccgc
     5881 gctgggtcac ctccacgccg tggagactgt accggcgttg gccgcgctcg ctctagaccc
     5941 cagcgactcg atgcggcaga gcgtcgtggc ggcgatcggc tccctgggca agtccagcac
     6001 gggctccgcg cgcacacggc gcggccttgc cggacggagc gggttcctgg gccgagcgat
     6061 ctggcggact gtctggtgga aaaagcgacc ggccctggct ccgcccggtg acccgatcga
     6121 gctggccgta gcaacgtgct ttgagaaagg cggccgcgcg ctcagtgccg acgctcgtcc
     6181 cgctcttgca ggaccgggat gagtcggtgc gcacggccgc ggccgaggct attgctcagg
     6241 tcggaacgct caatcacgcc gcgaccgcca ttttgtccct ggggctggat agtcgggaca
     6301 atgtcgttcg cgcccagacg gccgaggccc tcgggaccat cggcgcggcg gcagaggatg
     6361 ccgccccggc actcgtccag gcgacgatcg acggcaacga ccgcgttcgc gccaaagccg
     6421 tgcaagcgct gggcaagatc ggagagacgg ccgcggtcgt cgccgttccc ggcctggttc
     6481 gggcgctctc ggatcgagac aactgggtca gcgccttggc cgc
//
LOCUS       DATAQL010000065         7085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3212496, whole genome shotgun sequence.
ACCESSION   DATAQL010000065 DATAQL010000000
VERSION     DATAQL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212496"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VHS97_01055"
     CDS             <1..984
                     /locus_tag="VHS97_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446806.1"
                     /translation="EGAEAPAPGKPLFREFMGLNSHTIQFKPALYAPVCRAVRDYHPL
                     DWDTGDDTSFPPPFPSARNGVDWRKVYGSWKDAGYETDVSIMFDNLPAKSWKDLPRDA
                     RAYGRSFARSFGPSSASKLVASAEIGNEPGLYDDDAYRSLFRNMAEGLREGDPKLKIG
                     PCAVTLGKSERYAKSVDCLRGVEGLYDFLNIHTYAEVEGYPTWRRSYPEDPKIDYLRR
                     VNRLIEWRDRNAPGKEVRVTEFGWDASTKPRHTTGDFAKWDGNVSDEKQAQYLVRAFL
                     VFAAMDVNRAYLYFFNDADEPSFHASLGITRNFQPPGRATTSVVRLRPPAM"
     gene            complement(965..1279)
                     /locus_tag="VHS97_01060"
     CDS             complement(965..1279)
                     /locus_tag="VHS97_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446807.1"
                     /translation="MNLCSKPYVHLPIDAVDTVVFDRADRFEADRYDRYTSFAEARDA
                     ARSSIEIMLDEGDYDGEDHRQELANMLGLLDTCSTFEELALCPEYQRILEKLDLVTSL
                     AA"
     gene            complement(1450..2007)
                     /locus_tag="VHS97_01065"
     CDS             complement(1450..2007)
                     /locus_tag="VHS97_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX3446808.1"
                     /translation="MDRLYILYDARCGLCSWAKRWLMRQSMLIDLRFIPAGSATARRL
                     FPGLDKASEPPEELVVVSDQGAVYRNGSAWIMCLFALEAYRDWANRLAHPLLRPLARQ
                     GFALLSKQRSRISHWLSLASEVEIAQTLSQVSAPVCASQTSEPSVSSPLAATEKPSVR
                     PPLDGDSERFRSADDGNGPIHVSDY"
     gene            complement(2137..2550)
                     /locus_tag="VHS97_01070"
     CDS             complement(2137..2550)
                     /locus_tag="VHS97_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446809.1"
                     /translation="MNAYVVGTYITYLAISISLTVWVARTLVKNGRVFLVDSFLGNEA
                     LADSVNHMLAAGFYLVNVGYVSLALKYGDKPADLAQAIESLSTKVGLVLLVLGVMHFF
                     NLYVFSRMRRRALLRNQKPPVAAEQFLEPVQAWNH"
     gene            2860..3498
                     /locus_tag="VHS97_01075"
     CDS             2860..3498
                     /locus_tag="VHS97_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446810.1"
                     /translation="MWPDASETQSLLHRVARADVSATDELWERYRPALRQMIGLRLDQ
                     AVARRVDASDVVQDVLIKASQRLADYLRNPALPFHLWLRQIARDHVIDAHRRHRATAR
                     RTVDREQALYAHGGSFDDRSSLDLAAQIRDSGLTPAAEALRHELQTRFHATLLRLDDD
                     DREIILLRHFEQLSNSEAAQALGLSEAAAGMRHIRALRRLRTLLGETPSLCG"
     gene            3519..5279
                     /locus_tag="VHS97_01080"
     CDS             3519..5279
                     /locus_tag="VHS97_01080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3446811.1"
                     /translation="MTAPDDSMMSGQSADASLADADESLLAALLDELTDLWRQGQQPD
                     LERLASLHPRLTADLRSLWATVWVAEEMARNGFGEREPGEAHIGALNTTAWPQATECT
                     APAMAQATPHGLDGSLQGRFGDYELFEELGRGGMGVVSRAREIARGRIVALKRLLRGL
                     ASTPQDLERFQIEALAAARLAHPHIVPVFQVGECDGQPYFTMQYIDGTTLAQQLAGGP
                     LPEHDAARLLVPVCRAIHYAHDRGVLHRDLKPSNILIDRQGNPYVSDFGLAKRIDVDP
                     TLTPSGAFVGTPSYMPPEQAGRIASGRRDPLGATADVYSLGAILYHMLTGRPPFQAAT
                     PIETMLLALEHDPIAPRALNPRVNPDLEMVALQCLQKPPGLRYPSAAALAEDLESFLR
                     GDPVSARSTSLRALAARLMGETHHAPVLENWGLLWIYHSIALLVFFGTTNALYLAGVT
                     ARWPYVLIFTVGLCGWAALFWALRRRGGPIRFVERQLAHVWGAGIVSINLIFLVEWLL
                     GLPTLKLAPMIAVTNGTMFMIKAGILSGDYYLQAAVTFLAIFPMAWFPRFAPLIFGLV
                     ASACFFVTGLKYRLRRLRST"
     gene            5310..5936
                     /locus_tag="VHS97_01085"
     CDS             5310..5936
                     /locus_tag="VHS97_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3446812.1"
                     /translation="MPLARSVYNSNHPTDSGQSRASARRPLIEPEKTTARREPSREFL
                     GEQRAYFMTFDSKKQRKIMGQFATGVTVVTTGGEAGLHGLTANAIASLSLNPPLVLVA
                     IDKGAHSLDYLKKNRCFAVNILRQDQEAISRRFATPGPKNFADIDVATAATAAPILSD
                     CLAFVDCRVVEILPGGDHEIFVGEIVAGEFHGGEPLLYFAAGYRQLAE"
     gene            complement(5992..>7085)
                     /locus_tag="VHS97_01090"
     CDS             complement(5992..>7085)
                     /locus_tag="VHS97_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEX3446813.1"
                     /translation="MAVIAGTSRGAADDDRRRDGGPAPKGYVCYRASAPIRIDGRLDD
                     DAWKVAPWTDPFVDIQGDARPRPRFQTRAKMLWDDTYFYVAALLEEPHVGGTLTKHDS
                     IIFQDNDFEIFIDPDGDNHEYYELEINALNTEWDLFLKAPYRDGGPAINEWEIPGLKS
                     AVHVEGTLNDPKDTDKSWSVEFAIPWKALAERANRPAPPRDGDQWRVNFSRVEWRHEI
                     VDGHYRKVPKTPEDNWVWSPQGVIDMHRPERWGYVQFSTAMPGEAVYRVDAAGPIRDR
                     LMQIYYAQKAFFDRNKRWAATLGELQLADSPATASYATNLSLSPAGYEAAITYNPTGG
                     KPMTLTVQQDSRIQLRQANSPVTTSQSAP"
BASE COUNT         1278 a         2200 c         2220 g         1387 t
ORIGIN      
        1 gagggggccg aagcccccgc gccggggaag cccctcttcc gcgaattcat gggcctgaac
       61 agccatacga tccagttcaa gccagccctc tatgcgccgg tctgccgggc ggtccgcgac
      121 tatcacccac tcgactggga caccggcgac gacacgagct tccccccgcc gttcccctcc
      181 gcccgcaacg gcgtcgactg gcggaaggtc tacggctcct ggaaggacgc cggctacgag
      241 accgacgtca gcatcatgtt cgacaacctc cccgcgaagt cctggaagga cctcccgcgg
      301 gacgcccgcg cctatggccg gtcgttcgcc cgatcgttcg ggccgtcctc cgcgtcgaag
      361 ctggtcgcct ccgcggagat cggcaacgag ccggggctct acgatgatga tgcttaccgc
      421 agcctcttcc gcaacatggc cgaggggctg cgcgagggcg atccgaagct gaagatcggc
      481 ccgtgcgccg tgacgctcgg caagagcgag cgctacgcca agagcgtcga ttgcctccgc
      541 ggggtcgagg gcctgtacga cttcctcaac atccacacct acgccgaggt cgagggctat
      601 cccacctgga ggcggtccta tcccgaggac ccgaagatcg actacctccg ccgggtgaat
      661 cggctgatcg agtggcgtga ccggaatgca cccggcaagg aggtccgcgt gacggagttc
      721 ggctgggacg cctcgacgaa accccggcac acgacgggcg attttgccaa atgggacggc
      781 aacgtgagcg acgaaaagca agcgcaatac ctggtgcgcg cgttcctcgt gttcgccgcg
      841 atggacgtga accgcgccta tttgtatttc ttcaacgatg cggacgagcc ttcgtttcac
      901 gcctcattgg ggattactcg aaacttccag ccaccgggtc gcgcgacgac ctcggtggtt
      961 cggctcaggc cgccagcgat gtgacaaggt cgagcttctc gagaatgcgc tggtactcgg
     1021 ggcaaagcgc cagttcttcg aaggtagaac acgtgtcgag aaggccgagc atattcgcta
     1081 gttcttgccg gtgatcttca ccgtcgtaat caccctcatc gagcatgatc tcaatgctcg
     1141 atcgggccgc gtcgcgagcc tcggcaaaag aagtatagcg gtcgtaccgg tcggcttcaa
     1201 atcggtccgc gcggtcgaag accaccgtgt ccacggcgtc gatcggcaga tgaacgtagg
     1261 gtttactgca aagattcatg aacgattctg gctccccaaa aaaggattgt cgcaaattgg
     1321 atatctggca gtacgatcgg cggaccggct tggttcgccc agaaatggat cgatcccatc
     1381 ccgcggagat gagcccgttc acgccggaca cttgagtcca tcacgcggaa gagaaatcga
     1441 tccattcgat caatagtcgc taacgtgaat tgggccgtta ccgtcatccg ccgatcggaa
     1501 cctttcgctg tcgccatcaa ggggcggtcg aaccgatggc ttttccgtcg cggccaacgg
     1561 ggaacttacc gacggctcgg aggtttgtga cgcacagacg ggagcgctga cctggctcaa
     1621 ggtctgggcg atctcgacct cgctggccag tgagagccag tgcgagatcc tcgaacgttg
     1681 ttttgagagc agggcgaacc cctgccgggc cagaggtcgc aagaggggat gtgccagccg
     1741 gttggcccaa tcgcgatacg cctccagcgc gaataagcac atgatccagg cactgccgtt
     1801 gcgataaacg gccccctggt cgctgacgac aacgagctcc tccgggggct cactggcttt
     1861 gtccaggccg ggaaaaagcc gccgggccgt ggccgagccg gccggaataa acctcagatc
     1921 gatcagcatg ctttgccgca tcaaccaccg cttggcccaa ctgcagagcc cgcagcgtgc
     1981 atcatagaga atatagaggc ggtccatggt ttggctcctc attattgcgc acgcccggca
     2041 gaggtgcggc ggccaccggc agatcgccgc cagccgttct tgtcacacgg ccgttgcgtg
     2101 cgcccaacaa agggttggta ttgccgcggg tgcagttcag tggttccacg cttgtacggg
     2161 ctcgagaaac tgttcggcag caaccggcgg cttttgatta cgaagcaagg ctcgccgccg
     2221 catcctcgaa aagacgtaga ggttgaagaa atgcatcaca cccagaacaa gcagcaccag
     2281 acccaccttg gtgctcaggc tctcgatcgc ctgtgccagg tcggcgggct tatcgccgta
     2341 tttgagcgcg agcgagacat agccgacgtt cacaaggtaa aacccggcgg caagcatgtg
     2401 gttgaccgaa tccgccagtg cttcgtttcc caagaacgag tcgaccagga agactcgacc
     2461 gttcttcacg agcgttcgcg cgacccagac tgtcagcgat atgctgattg ccaagtaggt
     2521 gatgtaggtt ccgacgacat aggcgttcat gacccttgtc ctttgcactt gtccgcgtct
     2581 cctggaggag tctcggaacg ggcctggctg ccctggtccc ggcctatccg ggacgagatt
     2641 gctggcagcc cctctcacga acaagaaccg caagagtttc gcagcattaa ccgggaattc
     2701 tcttaaagaa gcgatgagaa ccgcgggtcc gccagcggtg gggagcatgg tttcatggta
     2761 gatcggactg gtatattggc acccgtaagc tacggcgcaa gtggccgggc gcgtggtgcc
     2821 tgatgaaagc tgtgttctgc caagagcttg gatcaactga tgtggcctga cgccagtgag
     2881 acacagagcc tgttgcaccg ggtcgcgcgg gccgatgtct cggcaaccga cgagctctgg
     2941 gagcgatacc ggcctgcctt gcggcaaatg atcggcctgc ggctcgacca ggcggtggca
     3001 cgacgggtgg atgccagtga tgtcgtgcaa gacgttttga tcaaggcgag ccaacggctg
     3061 gcggattatc tccgcaaccc ggccctgccg tttcatctct ggttgcgtca gatcgcgcgg
     3121 gatcacgtga tcgacgctca tcggcggcac cgcgcgacgg ccagacgcac cgtcgatcga
     3181 gagcaggcgc tgtacgccca tggcggttcg tttgatgatc gctcgtcgct cgatttggcc
     3241 gcgcaaatac gtgactcggg actgacccca gcggctgagg ctttgcggca tgagctgcag
     3301 actcgattcc atgccacgct tctccgcctc gacgatgacg atcgcgagat catcttgctc
     3361 cgccatttcg agcagctctc caacagcgag gccgcgcaag ctctgggtct ctccgaggcc
     3421 gccgccggca tgcggcacat tcgcgcattg cggcgactgc gaaccctgct gggggaaaca
     3481 ccgtctttgt gtggttgaga gagagagcct ttaaattcat gacagcgccc gacgacagca
     3541 tgatgtccgg ccagtctgct gatgcgagtc tcgccgacgc cgacgagtcg ctgctggctg
     3601 cgctcttgga cgagctgacc gacctttggc ggcaaggcca gcagccggac ctggagcgac
     3661 tggccagtct gcacccgagg ttgacggccg atctccgttc gttgtgggcg accgtctggg
     3721 tcgccgagga gatggccagg aatggattcg gcgaacggga gccgggcgag gcacatatcg
     3781 gtgctctgaa tacgaccgcg tggccccagg cgaccgagtg caccgcgcca gccatggcgc
     3841 aggcaacccc gcacggcctg gacggttcgt tgcaaggccg gttcggcgac tatgagcttt
     3901 tcgaggagct ggggcgtggc ggcatggggg tggtatcgcg ggcccgcgag atcgcgcgcg
     3961 gccggatcgt tgcgctcaag cggctgttgc ggggacttgc ctcgacgccc caggatctcg
     4021 aacggtttca gatcgaggcg ctggcggcgg cccgattggc gcatccccac attgtgccgg
     4081 tctttcaggt cggtgagtgc gacgggcagc cgtacttcac gatgcaatac atcgacggaa
     4141 ccaccctggc gcagcaactg gccggaggcc cgttgccgga gcacgatgcg gcgcggctgc
     4201 tggtgcccgt ctgccgcgcg attcactacg ctcacgatcg tggagtcttg caccgcgact
     4261 tgaaaccgtc gaacatcctg atcgatcgcc agggaaaccc ctatgtcagc gacttcggcc
     4321 tggccaaacg aatcgacgtc gatcccacgc tcaccccctc gggtgcgttt gtgggtacac
     4381 caagctacat gccgccggag caggccggcc gcatcgcctc gggccggcgc gacccgctgg
     4441 gcgcgaccgc cgatgtctac agcctgggcg cgatcttgta tcacatgctg accggccggc
     4501 cgccatttca ggccgccacg ccgatcgaga ccatgctcct ggccctcgaa cacgatccga
     4561 tcgcgccacg cgcgttgaac ccgcgggtca accccgacct cgagatggtc gcactccagt
     4621 gtctgcagaa gcctcccggg ctgcgttatc cgtcggccgc ggccctggcc gaggatctcg
     4681 agtcgttctt gcgcggcgat ccggtgtcgg cgcgctcgac cagcctgcga gcattggccg
     4741 cccggctcat gggcgagacc catcacgcgc cggtgctgga aaactggggc ttgctctgga
     4801 tctaccatag tattgcgctc ttggtctttt tcggcacaac taacgcgctg tacctcgcgg
     4861 gagtgacggc gcgctggcct tatgtattga tcttcacggt cgggctctgc ggctgggcgg
     4921 cgctcttctg ggcgctgcgc cgccgcggcg gtccgatccg cttcgtcgaa cgccagctcg
     4981 cccacgtttg gggtgccggg attgtctcga tcaacttgat cttccttgtc gagtggttgc
     5041 tgggattgcc cacgctcaag cttgctccaa tgatcgccgt caccaacggc acgatgttca
     5101 tgatcaaggc gggcatcctt tcgggcgatt attacctgca ggccgccgtg acgtttctgg
     5161 cgatctttcc catggcctgg ttccctcgat ttgcgccctt gattttcggc ctcgtcgcct
     5221 cggcatgttt cttcgtgaca ggtctcaaat accgcctgcg ccgcctgcga tcgacgtgaa
     5281 agggcacaaa cggcccgaga gcgtgccctt tgcctctcgc gcggagcgtg tacaattcca
     5341 atcaccccac cgactcgggc cagtccaggg ccagcgcccg tcggcccttg atcgagcccg
     5401 aaaagacgac ggctcgccgt gagccgtcgc gcgagttctt gggcgagcag cgagcttatt
     5461 tcatgacatt cgactccaag aaacaacgta agatcatggg ccagtttgcc acaggcgtca
     5521 ccgttgtaac gaccggcggt gaggccggtc tccatggcct gaccgccaac gcgattgctt
     5581 ccctttcgct caatccaccg ctggttctgg ttgcaattga caagggggcc cattcgcttg
     5641 actacctcaa gaaaaaccgc tgcttcgccg tcaacattct gcggcaggat caggaagcaa
     5701 tttcgcgccg ttttgcgacc ccggggccca agaacttcgc tgacatcgat gtcgccacgg
     5761 cggcgaccgc cgcccccatc ctgagcgatt gtcttgcgtt cgttgattgc cgggtcgtcg
     5821 agatattgcc tggtggagat cacgagatct tcgtcggcga gattgtggcc ggcgaattcc
     5881 acgggggcga gccgctcttg tattttgccg cgggttatcg ccagctcgcc gagtgacctg
     5941 attcgattgg tcctattcaa acaggtggcc gggtctactt gtcgccggct actagggcgc
     6001 ggactgggat gtcgttacgg gcgaattcgc ctggcgcaat tgaatccgcg aatcctgctg
     6061 aacggttaag gtcatcggtt ttccccccgt cgggttgtac gtgattgccg cttcatagcc
     6121 ggcgggcgaa agcgagaggt tagttgcgta actcgcagtc gctggcgaat cggcgagttg
     6181 caactcgccc agcgtggccg cccacctttt gttccgatcg aagaaagcct tctgggcgta
     6241 gtagatttgc atcaagcgat cgcggatcgg gccggcggcg tcgacgcggt agacggcctc
     6301 accgggcatg gctgtcgaaa actggacata gccccagcgc tcggggcggt gcatgtcgat
     6361 cactccttgg ggtgaccaca cccagttgtc ttccggcgtt ttcggcacct tgcggtagtg
     6421 gccgtccacg atctcgtgcc gccactctac ccgcgagaaa ttgacacgcc actggtcgcc
     6481 gtcacgtggg ggcgcgggcc ggttggcgcg ctcggccaaa gccttccagg gaatcgcaaa
     6541 ttcgaccgac cacgatttat cggtgtcctt gggatcgttg agcgttccct cgacgtgcac
     6601 cgctgacttc aatccgggga tttcccattc gttgatcgcc ggaccaccgt cgcggtacgg
     6661 cgctttcaaa aagaggtccc actcggtatt gagggcgttg atctcgagct cgtaatattc
     6721 atggttgtcg ccgtcgggat cgatgaagat ctcgaagtca ttgtcttgga agatgatcga
     6781 gtcgtgtttg gtcagagtgc cccccacgtg gggttcttcg aggagtgcgg ccacgtagaa
     6841 gtaagtgtcg tcccagagca tcttggcccg tgtctggaac cgaggccgag ggcgtgcgtc
     6901 cccctgaatg tcgacgaagg gatcggtcca cggggctacc ttccaggcgt cgtcgtcgag
     6961 gcggccgtcg atccggatcg gtgccgaggc gcgatagcag acgtaacctt tgggtgcggg
     7021 gccgccgtct ctacgtctgt cgtcgtcagc ggcgccgcgg gacgtgccgg cgatcacggc
     7081 catga
//
LOCUS       DATAQL010000066         6019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3364151, whole genome shotgun sequence.
ACCESSION   DATAQL010000066 DATAQL010000000
VERSION     DATAQL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3364151"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="VHS97_01095"
     CDS             <1..1758
                     /locus_tag="VHS97_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEX3446814.1"
                     /translation="PDIKDSAWFGGKAEGWERVPYWLDGMVPLAYLLDDSDLEAKVKR
                     AIDAILDRQQPDGWLGPVGDTQNHKPYDVWPLFPLFKALTQYQEASGDPRVLPALLKC
                     CRKIEQVISGEPLYSWAKVRAADLAITLYWLYDRTKEPWLLELADKAFAQSHDWRAMF
                     ENFPFKSKAQDRKDLENHGVNTGMGLKYGGVRYRLTGFANDKDAIFSMLDILDRYHGQ
                     ATGIFTCDEHLAGRSPSQGTELCTVAEAMYSLEVLAAVQGDPRLGDRLEKLAFNALPA
                     TFKKDMTAHQYDQQCNQVICSAAGEHDYTSNGPEANLYGLEPNFGCCTANMHQGWPKL
                     ASSLWMKTADDGLAVVAFAPCVIETKIQGKPVKVAVETMYPFRSVIAIAITVPEPMTF
                     PLYLRRPEWAKDAEIVLSNMKLNDGPARKSKYIKLSSEWSGTKTLNLRLPMDARLYQG
                     YKGAMAVERGPLVFALPINTDWKKFRDRPNLPFDDWEVYPKSPWNYALQIDREHPDRS
                     IAFEERAVGTSPFSTSSTPLVARVKGRRLAGWGLEKGAAAPPPESPLTSREPLEDLTL
                     IPYGCTDLRLTEFPILTTP"
     gene            1825..5283
                     /locus_tag="VHS97_01100"
     CDS             1825..5283
                     /locus_tag="VHS97_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX3446815.1"
                     /translation="MGVDDQTQPDLSVSPQKSRFRSSIVWKLTLFVGVLVALNCGVLI
                     GVAYLATSRILRNQIHQRLSTVAADRQEMLAYALRQHEERATHFAHRARFHQLLVQRA
                     EGTISSDRFKVDTDNLLLYARNSTTGYLALWIEDETGKVIASSGPQDLVAEYSRLDRS
                     EAGPDGGLVVPPRRVGTTFGFVFSSVVHGQDGEVLGIAFLLSDFAPIASFLMNPGGLN
                     ETGEVLVGVREGTNIRLVLPTREASTAAVVTASEFPTLDAAIVGKIGSTRTIDYRGHD
                     VLVDYRPVGLGYAGWGLVAKIDAREAYAPVDRLRTWLLALGGGALALGLGASNAIARR
                     FARPIRHLARTSAAIAAGDLSVRTLVNSSDEIGALSTAFNQMTEDLSRSYATLERRIS
                     ERTRDLEAVRDLLDAFFHISTSKLDHDNIDKTFDSVLRFCSQLGYDLAMISLVDHAAG
                     TIRAARATGKMTSVVDLTVRPLEGNDILAVAVRENRVLVIPDSRLDPLNDPAAVALSG
                     IRGQIVIPLVSDQVLGTLQVATHSPLDPHQVDLRPLETLAHHTARALTGIRQLEEIRR
                     LNQTLEQHAEELARSEAALREQTRILQSILDCMGDGVIVADSQASFIVFNPAAERMLG
                     HGRIDKPPQEWSRLYEVFLPDRVTPYPVEDLPLIRAIQGESTDQAELYIAYPSRDDGT
                     WILVTGRPVYDENDELQGGVIVFHDITRRKRFERRLAAQYETTRVLAEADTLAQANAK
                     ILETICESLDWDYGVFWRVDSHAQRLRCATIWHRPTASAPEFEALCRTISLQRGAGLP
                     GRVWENAQPAWIPEIASEADAPRRLVAMRDGLHAAFAVPIVLRGDCLGVLEFFSQEAR
                     PPDPAILEMMNNLGTQIGQFIERHQMRARVIQSEKLASLGMLSAGVAHEINNPLAYVA
                     NNLAVLERDVRFLLTLLAIYEKADQDLAFTQPDLIQQVARLSSEFDLSYVKEHMGKIL
                     RSTRQGVKRVADIVQNLRGFARLDYAAVDQADIHEALTTAIEMLRGRLDRRNITVEEN
                     LGTLPPVSCSPAQLNQVFLNLLVNAMQAIESTHRDDGRIEITTDSENGEIIVEVADNG
                     CGIPDEILPQIFDPFFTTKEVGDGTGLGLSITHSMVQDHGGRLEVESAPGQGTRFRVF
                     LPVARS"
     gene            5465..>6019
                     /locus_tag="VHS97_01105"
     CDS             5465..>6019
                     /locus_tag="VHS97_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446816.1"
                     /translation="MILSVIVILAILIDRAPGQDERPSHGPAAAARAPLARLVPKTDL
                     LLYFQFDGLDAHRDAWHASAAYKLLNDTRLGTVIEDLLLQVVEIFQDSEPREQRIASA
                     EIVDVLKQVARDGFVLGVTRIAQDDFRWVAAFRCGDRPVVRRLLEIAGAPGRPAMGQA
                     MADPAVISVGRHTFRRFGAEGVWLV"
BASE COUNT         1175 a         1837 c         1813 g         1194 t
ORIGIN      
        1 cccgacatca aggacagcgc ctggtttggc ggtaaggccg agggctggga gcgagtcccc
       61 tactggctcg acggcatggt tcctctcgcc tatttactcg acgacagcga tcttgaagcc
      121 aaggtcaagc gggcgatcga cgccatactg gatcgccagc aacccgacgg ctggctcggt
      181 cccgtgggcg acacgcagaa tcacaagccg tacgacgtgt ggcccctgtt cccgctgttc
      241 aaagcgctca cgcaatatca agaggcttcg ggcgatcctc gtgtgctccc ggcgctattg
      301 aagtgttgcc gcaagatcga acaggtgatt tctggcgaac ccttgtatag ctgggcgaag
      361 gtgcgcgcgg ccgacctcgc catcacgctt tactggctgt acgaccggac caaagagccc
      421 tggctcttgg aactagccga taaggcattt gctcagagcc acgactggcg tgccatgttc
      481 gagaatttcc cgttcaagtc caaggctcag gacagaaaag atctcgagaa ccacggcgtc
      541 aataccggca tggggctgaa atatggcgga gtgcggtatc ggctcacagg ctttgcgaat
      601 gataaggacg cgatcttcag catgctcgac atcctcgatc gatatcacgg ccaagccacc
      661 ggcatcttca cgtgcgacga gcatctggcg ggtcgcagcc cctctcaagg gacagagctt
      721 tgcaccgtgg ccgaagcgat gtactcgctc gaagttctgg cggctgtcca gggcgatccg
      781 aggctgggag accgcctcga gaaactcgcc ttcaacgccc tgccggcgac attcaagaaa
      841 gacatgacgg cccatcaata cgaccagcag tgcaatcagg tgatctgctc tgccgcagga
      901 gaacatgact acaccagcaa tggacccgag gcgaatctct acggccttga gccgaacttc
      961 ggctgctgca ccgccaacat gcatcagggc tggcccaaat tagcctccag cctgtggatg
     1021 aagacggccg acgacggcct ggcggtagtc gcgtttgcac cgtgtgtcat cgagaccaag
     1081 atccagggca agcctgtgaa agtggcggtc gagacgatgt atccttttcg ctcggtgatc
     1141 gctattgcga tcactgtgcc cgagccgatg acgtttcctc tgtatttgcg ccggcccgag
     1201 tgggcgaaag atgccgagat cgttctttcg aacatgaagc tgaacgacgg tccggctcga
     1261 aaaagcaaat atataaagct gagctccgaa tggagcggga caaagacgct aaaccttcgc
     1321 ttgcctatgg acgcgcgact ttatcaaggc tacaagggcg ccatggcggt cgagcgtggt
     1381 ccgctggtct ttgccctgcc gatcaacacc gattggaaaa aattcaggga tcggcccaat
     1441 cttcccttcg acgactggga ggtgtatccg aaatccccct ggaattacgc gctccagatc
     1501 gaccgagaac accccgatcg atcgatcgcc tttgaggaac gggcggtcgg gacaagcccg
     1561 ttttccactt cgagcacgcc gctcgttgcc cgagtcaagg gtaggcgtct tgccggctgg
     1621 ggtctcgaaa aaggagccgc ggcaccccct ccagaaagcc ctttgacaag ccgggagccg
     1681 ttggaggatt tgactctgat cccgtacggc tgcaccgacc tgcggctcac cgagttccca
     1741 attcttacca caccgtgaga tgatagacgg gtcgccggcg ctgccggttc tggttgaagc
     1801 cgcgaaagca ccgagtctgt gtcaatgggt gtcgatgatc aaacccagcc ggatttgtca
     1861 gtctctccgc agaaatcgcg atttcgctcc tcgatcgtct ggaaactgac attatttgtg
     1921 ggcgtcttgg tcgcgctcaa ttgcggtgtg ctgatcggcg tcgcgtacct tgcgaccagc
     1981 aggatccttc gcaaccagat ccaccaacgg ctgtcgaccg ttgcagctga ccgccaggag
     2041 atgctggcct acgctcttcg gcagcacgag gagcgggcga cgcacttcgc acatcgcgct
     2101 cgtttccacc agcttctcgt ccaacgcgcg gaggggacga tcagctctga ccgattcaag
     2161 gtcgataccg acaacctcct gctctatgcg cggaactcga cgacagggta tctggccctc
     2221 tggatcgagg atgaaaccgg caaggtgatc gcatcgagcg gccctcaaga tctcgttgcc
     2281 gaatattctc ggttggatcg atctgaggca ggaccagatg gcggtctggt ggttccgccg
     2341 cgtcgcgtgg gcacgacttt cggctttgtg ttttcttcgg tggtccacgg tcaggacggc
     2401 gaggtcctgg gcatcgcttt cctgctgtcg gatttcgcgc ccattgcatc gtttctgatg
     2461 aacccgggcg ggctgaatga aaccggcgaa gtcttggttg gcgttcgtga agggacgaat
     2521 atccgcctcg ttttgcccac ccgggaagcg tctactgccg ccgtggtgac ggcgagcgag
     2581 ttccccactc tggacgcggc gatcgtggga aagattggct cgacgcggac aatcgattat
     2641 cgcggccatg acgtgctggt ggactacagg cccgtcggtt tgggctacgc gggttgggga
     2701 ctcgtcgcca agatcgacgc ccgcgaagcg tatgcgccgg tcgaccggct ccgcacttgg
     2761 ttgcttgcgc tcggcggggg agccctggca ctgggattgg gagcgtccaa cgcgatcgcg
     2821 cggcggtttg cacggccgat ccgccatctg gcccgcactt cggcggccat cgctgccggc
     2881 gatctgagcg ttcgcaccct ggtcaattca tcggacgaaa tcggtgcctt gagcacggcg
     2941 ttcaaccaga tgaccgagga cctttcgcgc tcctacgcca cccttgagcg ccggatctcc
     3001 gaacgaacgc gcgatctgga ggccgtacgt gacctgctcg atgccttttt ccacatctcg
     3061 acctcgaagc tggaccacga caatatcgac aagacgttcg attcggttct gcgattttgc
     3121 tcccaactgg gttacgacct ggcgatgatc tcgctggtcg atcacgctgc cggcacgatc
     3181 cgggcggcgc gtgcgacggg gaaaatgacc agcgtggtgg acctgacggt ccgccccctc
     3241 gaagggaacg acattcttgc ggtcgcggtg cgagagaatc gcgtgctggt catccccgat
     3301 tccaggctcg atccactgaa cgacccggcc gcagttgcct tgtcgggcat ccgcggccag
     3361 atcgtgattc ctctggtcag cgatcaagtg ctgggcacgc ttcaggtagc cacgcattcg
     3421 cccctagacc cacatcaagt ggatttgcgc ccgctggaga ctctcgccca tcacaccgct
     3481 cgggcattga cggggattcg tcagctcgag gagatccgac ggctcaacca aaccctggaa
     3541 cagcacgccg aggaactggc gcgctcggaa gccgcgctcc gagagcagac ccgaatcctc
     3601 caatcgattc tcgattgtat gggcgacggg gtcatcgtcg ccgactcgca agccagtttc
     3661 atcgttttca acccggctgc cgagcgaatg cttggccacg gacgcatcga caagccgccc
     3721 caggaatggt cgcggcttta cgaggtcttc ctgccggatc gggtgacccc atacccggtg
     3781 gaagaccttc ccttgatacg cgcaattcag ggcgagtcga ccgaccaggc cgagctctac
     3841 attgcttatc ccagccgcga tgacggcacg tggattcttg tgaccggccg ccccgtgtac
     3901 gacgaaaacg acgagctgca aggcggggtc atcgtcttcc acgacatcac acggcgcaag
     3961 cgatttgagc gccgactggc cgctcaatac gagacaactc gggtgctggc cgaggccgat
     4021 acactcgccc aggcgaacgc caagattctc gaaaccatat gcgaaagcct cgactgggac
     4081 tatggcgtgt tctggcgagt cgactcgcat gcccagcgct tgcgatgcgc gacgatctgg
     4141 catcgaccca cggcgtcggc gcctgagttc gaggctctct gccgcacgat atcactgcag
     4201 cgtggagctg gactgccggg ccgggtttgg gagaacgccc agccggcctg gattccggag
     4261 atcgccagcg aagccgatgc cccccgacgc ttggtcgcaa tgcgagatgg tctgcatgcg
     4321 gcctttgctg tgccgatcgt tttgcgtggc gattgtctgg gggttctcga gttttttagc
     4381 caagaggcgc ggccgcccga ccccgccatt ctcgaaatga tgaacaacct gggcacgcag
     4441 atcggccagt tcatcgagcg gcatcagatg cgagcccgcg tcatccagtc agaaaaactg
     4501 gcgtcactgg ggatgctttc ggcaggagtg gcccatgaaa tcaacaatcc cttggcttac
     4561 gttgccaaca acctggcagt gctcgaacgc gatgtccgat tcttgctcac gctgctggcg
     4621 atctacgaaa aagccgatca agacctggcg ttcacgcagc ctgacttgat tcagcaggtc
     4681 gcacggctct cctctgagtt cgatctgagc tatgtgaagg aacatatggg caagattctg
     4741 cgtagcacgc gccagggggt caagcgcgtg gccgacatag tccagaacct ccgcgggttt
     4801 gcccgcctcg actacgccgc cgtggatcag gctgacattc atgaggcgct cacaacggcg
     4861 atcgagatgt tgcggggccg cctcgaccgg cgcaacatca ccgtggagga aaatctgggg
     4921 actttacccc cggtctcctg ttcgcccgcc cagctcaatc aggtcttctt gaacttgctc
     4981 gttaacgcca tgcaggcgat cgagtcgacc catcgcgacg acggccgaat cgagattact
     5041 accgactcag agaacggtga gataatcgtc gaggtggccg acaacggctg cgggatcccc
     5101 gacgagatcc ttccccagat cttcgatccc ttcttcacga ccaaggaggt aggtgatggc
     5161 accggattgg ggctttcgat cacgcacagc atggtccagg accacggtgg tcgtctggaa
     5221 gtcgagagcg cacccggcca gggcacgcga ttccgcgtct ttcttccagt cgccaggagc
     5281 taaggctccg cgaaattata agttgagcga acttcggccc cggagtggca cgggcttcca
     5341 gcccgtgtct gaaaatacca cgggcaagat gcccgtgcca cttaagcccg ccgcgcccgc
     5401 tggctcaact tgttctttcg cggtgcctga gtcgcggact atcagtttcc gggtagtccg
     5461 cggcatgatc ttaagcgtga tcgtgatcct ggcgattttg atcgatcgag cgcccggcca
     5521 ggacgaacga ccttcgcacg gcccggcggc cgccgccaga gcgcccctgg cgcgcctggt
     5581 tccgaaaacg gatttgttgc tctacttcca gttcgacggg cttgacgccc atagagacgc
     5641 ctggcacgcg tcggccgcct acaaactctt gaacgacaca aggctaggca cggtcatcga
     5701 agacctcctg cttcaagtcg tggaaatctt ccaggattct gagccccgcg agcagcgaat
     5761 cgcgtctgcg gaaatcgtcg acgttctcaa gcaagttgcc cgcgacgggt ttgtgttggg
     5821 tgtaacgagg attgctcaag acgactttcg ctgggtcgcg gcgttccgat gtggcgaccg
     5881 accggtggtc aggcggttgc tcgaaatcgc tggagccccc ggacgccccg cgatgggcca
     5941 ggcaatggct gatcccgccg tgatctcggt cggccgccat acgtttcgcc ggtttggcgc
     6001 cgaaggagtt tggttggtc
//
LOCUS       DATAQL010000067         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_159156, whole genome shotgun sequence.
ACCESSION   DATAQL010000067 DATAQL010000000
VERSION     DATAQL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159156"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..856
                     /locus_tag="VHS97_01110"
     CDS             134..856
                     /locus_tag="VHS97_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3446817.1"
                     /translation="MLQPQTLSRQVYQQLLRQIFSGELTPGSPLREAELSAQLGVSRT
                     PVREALGRLSEYGVVESRPNHGCVVCRLGREQLIHLHEVREALEGMAIELACGKLTNA
                     DFAYLDELAEAARDQSSPNYFSACDEFDVKLHGMIAERSGNPILAREIRKLQDMTMMI
                     HDQLESVLIGGRRVDPEEQWEIRRNGWDEHVRIIDALRSRKPKACRAAMVSHIRTTSE
                     YKARLMPASDSGPNENGRSARH"
     gene            1521..2663
                     /locus_tag="VHS97_01115"
     CDS             1521..2663
                     /locus_tag="VHS97_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3446818.1"
                     /translation="MTRRSHRAFTLIELLVVIAIIAVLIALLLPAVQSAREAARRIQC
                     TNNLKQIGLASHNYHTAINSFPLGSSMNPNCNTTAPYPIANWGANQSSLSLLLPFIEA
                     GPLFNSMNYSCMADPAYGPANRTATLSYINVFICPSDPNISNHDCNCNYAACYGTTTN
                     SMTSTGTSMSWSLGSSPAGTFTGSTGLYAPAVSYSIANCTDGTSNTVAYAETLVGDSK
                     ATAVYLDSSSPGRAISPPSTYRGNIVYTSNAVVKLQDAWTNQPATLAGLQICATAMMG
                     PGALIQDTRGYRWAFGITGYTMFNTIQTPNDSQYPFGGCRPFDTNTTNYPDDGFSYDA
                     NSAHPGGVNTLFGDGSCRFVKNSIARNIWWSLGTKAGGEIVSSDSY"
     gene            2780..>3105
                     /locus_tag="VHS97_01120"
     CDS             2780..>3105
                     /locus_tag="VHS97_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446819.1"
                     /translation="MTTSVQEWSTCAVSAPVGRQNSRRSRQAPAGWGACIWLVPIPLV
                     LVVMTAFFPALNNGFVNWDDDKNFLDNPYYRGIGAAQWKWAWSTFWLGVYQPLAWLLF
                     EVQYFF"
BASE COUNT          632 a          895 c          896 g          682 t
ORIGIN      
        1 attcgaatcg cggggaaacc tttcctcagc ctggtagcat cgagacctct gacaaaatcc
       61 agaggaatct cttgaccaat gattgtatac aatatacaat gcggccgctg cccatctcta
      121 aaaggtatct gtcgtgcttc aaccacaaac gttgtcgagg caagtttatc agcaacttct
      181 gcggcaaatc ttctccggcg agttgacgcc cggcagcccg cttcgggagg ctgagttatc
      241 cgcccagctc ggggtcagcc gcacgcctgt gcgcgaggca ctgggtcggc tgtccgagta
      301 cggtgttgtc gagtcgcgtc caaaccacgg gtgcgtggtg tgccggttgg ggcgcgagca
      361 gttaatccac ctgcacgagg ttcgcgaggc actagagggg atggcgatcg agctcgcatg
      421 cggcaagctc accaacgccg atttcgccta tctcgatgaa ttggccgagg cggcgcggga
      481 tcaatcgtcg cctaattact ttagtgcttg cgacgaattt gacgtgaaac tccacggcat
      541 gatcgcggaa cgctccggga atccgatcct cgctcgtgag atccgcaagc tccaggatat
      601 gacgatgatg atccacgatc agctcgaatc agtcctgatc ggtggtcgtc gtgtcgatcc
      661 tgaagagcaa tgggagatcc gacggaacgg ttgggatgaa cacgtcagga tcatagatgc
      721 cttgagatcg agaaagccaa aagcttgtcg cgcggccatg gtcagccaca tccgcacgac
      781 gagcgagtac aaggcccggt tgatgcctgc gtctgattcg ggcccaaatg agaacggcag
      841 gtccgcgcgg cactgaatta gcctaggcgc agacgggacg cgcgatgcgt ctagattcaa
      901 gctctcattc gcgatcgggg gtcggtccca gagcgaagcc gtcgttgaga agttgatcga
      961 cacgtcgcat cacccagcgc ttatctgccc ttgtgattgt gcgcgcggtt taagcacttg
     1021 agttcacgag agtttgttcg ctctgggcac tggcgggcat ttggcccggc atccactagt
     1081 gagcgtgctt cgagtcgagt cggtacatct ttttcatttt caagttcagc tccccggagg
     1141 acactgatgc tgcttcgttg cagggtttct tgcctcgcca cgatcggttt tctcttgctt
     1201 tgcatgatcg ggtgcggggg tggagtgcca acgaccggag agtcgctgaa agaagggccg
     1261 gataccgcag caaggggaaa ggcaatggcg gatggataca tggcaaaggc tggaatgcgc
     1321 aaggatgaag cccgcgcgaa aacgccagcc ccgccgaaat aggcgcatcg tccggcctat
     1381 catccctcga tcgtttgacg ggatccgcgt tcagaagtgt cggaacggtg aatcaatagg
     1441 cgtgcctccc tcgtcggcgt ctagccgctg attcacaggg cctcggttca tttattctct
     1501 tcgtaaacgg agcgatcatc atgactcgtc gttcgcatcg tgccttcacc ttgatcgagc
     1561 tcttggtcgt cattgcgatc atcgccgtgt tgatcgccct gctactgccg gcagtgcagt
     1621 cggcccgcga ggctgcgcgg aggattcaat gcaccaacaa ccttaaacag atcggcctag
     1681 ccagtcacaa ttaccacacc gccatcaatt cgttcccgtt gggatcgtcc atgaacccca
     1741 actgtaacac gacagcgcca tatccgattg cgaattgggg agccaaccag agctctctct
     1801 cgttgctttt gccgtttatc gaggcgggcc ctctcttcaa ctcgatgaat tactcctgca
     1861 tggccgaccc agcgtatggt ccggctaacc gtacggccac gttatcctat attaacgtgt
     1921 tcatttgccc atcggacccg aatatctcca accacgactg caactgcaat tatgccgcct
     1981 gctacggaac cacgaccaat tcgatgacgt ccacgggcac gtcgatgagc tggagcctcg
     2041 gctcgagccc ggcaggtacg ttcactggca gcacggggct gtacgcgccg gcggtgagtt
     2101 actcgatcgc caattgcacc gacggtacat cgaacaccgt tgcttacgcc gagacccttg
     2161 tcggagacag taaggcgacg gctgtgtatt tggactcgtc atcgccggga cgagccattt
     2221 cgccaccgag cacatatcgt ggaaatatag tttacacctc gaatgcggtg gtcaagcttc
     2281 aggacgcctg gacgaaccaa ccggcgacct tggctggtct ccagatctgc gcgacagcga
     2341 tgatgggacc gggcgccttg atccaggaca ctcgcggtta tcgctgggcg tttggcatca
     2401 cgggttacac gatgttcaat acgattcaga cccccaacga ttcacagtat cccttcggcg
     2461 ggtgccgtcc gtttgatacc aacacgacca actatccgga cgacggtttc tcctatgatg
     2521 ccaacagcgc tcatcccggt ggtgtgaaca cgctctttgg tgacgggagc tgcaggtttg
     2581 tgaagaattc gatcgcccgg aacatttggt ggtcgctcgg caccaaggcc ggcggcgaaa
     2641 tcgtcagctc ggacagctac tgagccacgt ggttgcccgg cctctccgcc ggcgagatcg
     2701 gctcaagagt agatcgttcg tgtcttcagc tctgcgtccg gtgatttgag aaacggatgc
     2761 cggtcgctct tgattgatca tgacgacttc cgtgcaagaa tggtcaacgt gcgcggtctc
     2821 cgcgccggtc gggcgccaga attccaggcg gagtcgccag gcgccggcag gctggggcgc
     2881 ttgcatctgg ctggtcccga ttccgctcgt gctcgtcgtc atgaccgcct tcttcccggc
     2941 cctgaacaac ggcttcgtca actgggacga cgataaaaac ttcctcgaca acccctacta
     3001 ccgtgggata ggcgcagccc agtggaagtg ggcctggagt acgttttggc tcggggttta
     3061 ccaaccgcta gcctggttac tcttcgaggt gcaatatttc ttctg
//
LOCUS       DATAQL010000068         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2449270, whole genome shotgun sequence.
ACCESSION   DATAQL010000068 DATAQL010000000
VERSION     DATAQL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449270"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VHS97_01125"
     CDS             <1..580
                     /locus_tag="VHS97_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3446820.1"
                     /translation="CGLLVNKIGGPSVKPYQPAGYWSLLNFPVRDYISDHGESQYRRG
                     LYSYWQRTFPHPSMIAFDAPSREECTAERPRSSTPLQALVLLNDPSYVEAARVFAARI
                     IRHGGGSFPEKLAWAFRTALSREVQPEEVRVISKLYTKHMKDYKCDKQAAKQVVSVGE
                     SPVPTDLDIGDFAAWTSVARVILNLHETITRN"
     gene            583..2022
                     /locus_tag="VHS97_01130"
     CDS             583..2022
                     /locus_tag="VHS97_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3446821.1"
                     /translation="MTRFERLRLAVTRRSFLGRSATGLGAVALASILNPRLLADSASG
                     DSGERWRGVVNPLHHAAKAKRVIWLYMAGGPSHLETFDYKPKLAELSGKPMPESITKG
                     QPIAQLQGARLTCMAPQHKFRRYGKSGQEFAVLFPNLGEVADEMCIVRSLQTDAINHD
                     PAHTFMNTGTSISGRPSMGSWLWYGLGSDCDDLPGFVVLISTGKAGQNQPIAARQWHS
                     GFLPGSFQGVSFRSKGDPVLYVNSPQGVSQSKQRDIVDAAMTLNKIHNDSVDDPEIAT
                     RISQYEMAFRMQSSVPGLMDLSKEKKETLEMYGTSGSDGSFAANCLLARRLAERGVRF
                     IQLYHRDWDHHGNVKNDVAVTAREVDQPTAALIKDLKRLDMLKDTLIIFGGEFGRTPM
                     AQGTGRDHHMKGFSMWMAGGGIKGGISYGSTDEFGYNAIDPVVHVHDVHATMLHLMGI
                     DHEKLTYLYQGRQFRLTDVSGNVVNGILA"
     gene            complement(2264..2896)
                     /locus_tag="VHS97_01135"
     CDS             complement(2264..2896)
                     /locus_tag="VHS97_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446822.1"
                     /translation="MPGPQEVVIIAADAVRVQGKIRPLDDAKAVSGRALYVGGKATVP
                     TTPAVTAEYLPPHTDRGRKTTGVSEFDLKLDPKNVGVMLRRKLDYALPNQRAQVFVAD
                     ASRAGADAGELDWKPAGVWYLAGSNTCVYSNPKDELGAAQHVAQTSNRRFRDDEFLVP
                     RALTAGRSAIRVRVRFTPVSRPLCPGERLPELAWSEIRYTAYCWVIPDES"
     gene            complement(2941..4353)
                     /locus_tag="VHS97_01140"
     CDS             complement(2941..4353)
                     /locus_tag="VHS97_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446823.1"
                     /translation="MADSTSPRRTFPTLLLFCLTAGIAAAGEPPTIPIGLDAYRQWDR
                     WPSQRLGARAYMRSTYDRRGGNEGADASHFLYQTADDFNVTLDALGPGVLYFARYNHW
                     HGGPWHYEVDGTDHIVRETSSADPNHPAPNSVFLPQKLFPNPLTWTWAITKGADLMWV
                     PLPFEKSFRMAYSRTHYGTGYYIFHQYVPGAPLSRPIRSWDGQTPPDKDVLDLVARAG
                     TDLVPRADSLDGIRSGMHERSGVVKLPSKGTVALGEIIQAPSTIRALEFDLPAGKAEE
                     FGRALLRVTWDGRPGPSIEAPVALFFGAGTLYNRDDREYLVKAFPVFIRFASGRARLA
                     CFFPMRRATSGASIPPASRVSNDLSPPLTTSPSASSRPRRASRTRSGSGERTLTETTR
                     PTPSGCSSTTRSERPVWARAPRIPRASATGSTGFQRALTRGQVPCPRIRPRRSPSRAV
                     ANIVSASSHATPSMSSIRSG"
BASE COUNT          864 a         1388 c         1467 g          847 t
ORIGIN      
        1 ctgcggttta cttgtgaaca agatcggcgg accgagcgtc aagccctatc agcccgccgg
       61 ctactggtca ctcctgaact tcccggttcg cgactatatc tccgaccacg gcgagagcca
      121 gtatcgacgc gggctctata gctactggca acggacattt ccgcatccga gcatgattgc
      181 gttcgatgcg ccgagtcgag aagaatgcac ggccgagcgc ccgcgctcga gcacgccgct
      241 gcaggccctc gttctgttga acgatccctc ttatgtggaa gcggcacgtg tgttcgccgc
      301 gcgcattatt cgccacggag ggggctcgtt tcccgaaaaa ctcgcttggg ccttccggac
      361 cgcgctctca cgcgaagtac agcccgagga agtgcgcgtg atatcgaaat tgtatactaa
      421 acatatgaaa gattataagt gtgataagca ggcggccaaa caggttgtga gtgtcggcga
      481 atcgccggtc ccgacggacc tcgacatcgg cgacttcgcg gcctggactt ctgtggcgcg
      541 ggtgattctc aacttgcacg agaccatcac gaggaactaa agatgacacg attcgaaagg
      601 ctccgactcg cagtcacgcg ccgatcgttc ctcggccgtt cggcgacggg tctaggcgcc
      661 gtcgcccttg catcgatcct caatccacgc cttctggcag attctgcatc cggggacagt
      721 ggggaacgct ggcggggggt ggtgaatccc ttgcatcacg ccgccaaggc gaaacgagtc
      781 atctggctct acatggccgg tggtccctcg cacttggaga cattcgacta caagcccaag
      841 ctggccgagt tgagtggcaa gccgatgccc gagtcgatca caaagggaca gccgatcgcc
      901 cagcttcagg gcgcccggct cacctgcatg gcgccgcaac acaaattcag aagatacggc
      961 aagtcaggac aggaatttgc cgtgttgttc ccgaatcttg gcgaggtcgc ggacgagatg
     1021 tgtatcgtcc gttcactcca gaccgacgcc atcaaccacg atccggccca caccttcatg
     1081 aacacgggca caagcatctc cgggcgccca agcatgggct cgtggctgtg gtacgggctt
     1141 ggcagcgact gcgacgatct ccccggtttt gtggtgttga tctcaacggg caaggcaggc
     1201 cagaaccagc ccattgcggc ccggcaatgg cacagcggct tcctgcccgg cagtttccag
     1261 ggggtctcct ttcgctccaa gggagaccca gttctctacg tcaacagccc gcaaggggtg
     1321 tcacagagca agcagcgcga catcgtcgat gccgcaatga cccttaacaa aatccacaac
     1381 gactcggtcg atgacccgga gattgccact cgcatcagcc agtatgaaat ggccttccgc
     1441 atgcagtcga gcgttccggg actgatggac ctttccaagg agaagaagga aactctcgaa
     1501 atgtatggta cctccgggtc ggacggttcg ttcgctgcca actgtctgct tgcccgccga
     1561 ttggccgagc gcggggtccg ctttattcag ctttatcacc gcgattggga tcatcacggc
     1621 aatgttaaaa acgacgtggc cgtcacggcc cgggaagttg atcagcccac ggccgcgctc
     1681 atcaaagatc tcaaacgcct cgatatgctc aaagacacgc ttatcatctt cggcggcgag
     1741 ttcggccgaa ctcccatggc ccagggcacg ggtcgcgatc atcacatgaa gggtttctcc
     1801 atgtggatgg ccggcggcgg catcaaggga ggcatcagct acggctcaac cgacgagttc
     1861 ggctacaacg ccatcgaccc agtcgttcat gtccacgacg tacatgccac catgttgcac
     1921 ttgatgggca tcgatcacga gaaattgacc tacctctatc agggccggca gtttcgcctg
     1981 acggatgtgt ccggaaatgt ggtgaatgga attctcgctt gattgccgag ggatcgtgat
     2041 tgggcgttca ccaagacgag cgattagtaa ttcttacgtg agtgagcgtt tgtgcgtcca
     2101 ctggtgcaag gccccgggac aagtcggtcc gtgagatatg ggcgaagcgc ggctacacat
     2161 ccgcactcat caacgggcag gcgaatgttc gatgtgatcc ctaagtcagg atcgtgagcg
     2221 atcctgtgtg attgagcccc tgccggttcc gagacgggcg atctcacgac tcgtcaggta
     2281 tcacccagca atatgcggtg taacggatct cgctccaggc cagttccgga agccgctcac
     2341 cggggcacag tggcctcgac acgggggtga agcggacgcg cacccggatc gcggaacggc
     2401 ccgcggtcag cgcgcgcggc accaggaatt cgtcgtcgcg gaagcggcgg ttcgaggttt
     2461 gagcgacgtg ctgcgccgcg ccgagctcat ccttcgggtt ggaatagacg cacgtattcg
     2521 atccggcgag ataccagacg ccggcaggct tccagtcgag ctcgccggcg tcggcgccgg
     2581 ctcgactcgc gtcggccacg aacacctggg cgcgctggtt gggcagggca tagtcgagct
     2641 tccgccggag catcacgccg acattcttgg gatcgagttt caagtcgaat tcggagacac
     2701 ccgtggtctt ccggccgcga tcggtgtgcg gcggaagata ctcggcggtg acggcgggag
     2761 tcgtgggcac ggtggccttg ccgccgacgt agagcgcccg gccggagacg gccttcgcat
     2821 cgtcgagggg acggatcttg ccttgaacgc ggacggcgtc cgcggcgatg atgacgacct
     2881 cttgcgggcc cggcacgggc gcttgctcgg agggctcggg cagaatcgtt cgcgtcgtgc
     2941 tcagccagat ctgatcgagg acatgctggg ggtggcgtgg ctggatgcgg agacgatgtt
     3001 tgccaccgcg cgggaaggtg accgtctggg gcggatcctg gggcagggca cttgaccacg
     3061 cgtaagtgcc cgctggaaac cggtcgagcc agttgccgaa gcccttggga tgcgcggagc
     3121 ccgcgcccag acgggtcgtt ccgatctcgt cgtcgaactg catccagaag gcgtcggacg
     3181 tgttgtctcc gtcaaggttc tttccccgga cccagatcgt gtacggcttg cccgcctcgg
     3241 cctggaactc gcactcggcg atgtcgtcag gggcggcgag aggtcgttcg acacccgaga
     3301 cgcgggcgga atcgacgccc cactcgtagc gcgacgcatc gggaagaagc aggcgagccg
     3361 ggcgcgcccg gacgcgaacc ggatgaacac cgggaacgcc ttgaccaggt attcgcggtc
     3421 gtcccggttg tacaacgtcc ccgcgccgaa gaagagcgca acgggggcct cgatcgaagg
     3481 tccggggcgt ccgtcccacg tcacgcggag caacgcccgg ccgaactcct ccgccttccc
     3541 ggcggggagg tcgaattcca gtgcccggat cgtcgacggc gcctggatga tctccccgag
     3601 cgccacagtt cctttcgagg ggagtttcac gacgcccgac cgctcgtgca tcccggaccg
     3661 gatcccgtcg agcgagtcgg cccgggggac gaggtcggtc ccggcccgtg cgaccaagtc
     3721 cagcacgtct ttgtccggag gagtctggcc gtcccacgac ctgatcggcc gcgacagagg
     3781 ggccccgggg acatactggt gaaagatgta gtagccggtg ccgtagtgcg tccgcgagta
     3841 ggccatgcgg aacgacttct cgaacggcag cggcacccac atcaggtcgg cacctttggt
     3901 gatcgcccac gtccaggtca gcgggttcgg aaacagtttc tggggcaaga agactgagtt
     3961 cggcgcgggg tgattcgggt cggccgacga cgtctcccgg acgatgtggt cggtaccgtc
     4021 gacctcgtaa tgccagggac cgccgtgcca gtgattgtat cgcgcgaaat aaaggacacc
     4081 cggccctagg gcgtcgagcg tcacgttgaa atcatcggcg gtctggtaga ggaaatgact
     4141 ggcgtcggcg ccttcgttgc cgccccttcg atcgtaggtg cttcgcatgt aggcgcgcgc
     4201 gcccaaccgc tgtgagggcc atcggtccca ctgtcggtag gcgtcgagcc cgatcgggat
     4261 cgtcggtggt tcgcccgccg ctgcaatgcc cgcggtcagg caaaacagca gcagtgtcgg
     4321 gaaggtccgt cgtggcgagg ttgagtcggc catggattcg tattccgccc ctcgttttag
     4381 gattcggggt gaggctagag cgagattgtc ccgcctgtag ttgaaatgtg gggcggctcc
     4441 tcacggacat tctcacgcca ctccgtgacg gtatccagtc cttatgccca aaccagagtc
     4501 ttcggggtcc tgcgacctcg gcgtcgtgcg atggcctcgt cacaccagga gaagccgaga
     4561 tcggcc
//
LOCUS       DATAQL010000069         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2449341, whole genome shotgun sequence.
ACCESSION   DATAQL010000069 DATAQL010000000
VERSION     DATAQL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449341"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VHS97_01145"
     CDS             complement(<1..577)
                     /locus_tag="VHS97_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX3446824.1"
                     /translation="MAGSTTAFGPVILKPRRARPFFARHPWVFDSSIDRVIGEPRAGD
                     QVDVVTNDGQRIATGLYNPTSTIRVRLYRWDDGPLDGAFWSKTLAAAGRLRSEILDLG
                     KNSSAYRLIFSEGDGLSGLTVDRYDRWLVAQFTSLALYTRRDSILKLLMELTGALGII
                     ARTDRGIAHKEGLRAGEEVIVGSIPDLPVEIV"
     gene            complement(673..1140)
                     /locus_tag="VHS97_01150"
     CDS             complement(673..1140)
                     /locus_tag="VHS97_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3446825.1"
                     /translation="MDHSPGAPKIVIADDNYQNVELLEAYLSDFDCEIRTAHDGEETL
                     LVVDEFKPDLLLLDVMMPRLSGFEVCKKLRSNADTKDLLILMVTALNEAADFERGVQV
                     GTDDFLTKPVNKVELLCRIRSLLRVRHLKTQLDRTLAYLADFEATSRESSSNP"
     gene            1490..2074
                     /locus_tag="VHS97_01155"
     CDS             1490..2074
                     /locus_tag="VHS97_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446826.1"
                     /translation="MPLRDIDRKLVDRCLQKEPGAWNDFVDRYMGLIYHVIQHVAYAR
                     SRLLSSEDVEDIGAEILLKIVDNDYAVLKKFKGISSLPTYLTVIARRTCVKELIKRHR
                     EEELSHTSAHRAFTDDGVSGEVEAILSAEEVGRMLEDLSDREAEVIRLYHLKFLNYRQ
                     IGKQLGLPENSIGPILAKARAKLRHAAEKQQSAS"
     gene            complement(2104..>2418)
                     /locus_tag="VHS97_01160"
     CDS             complement(2104..>2418)
                     /locus_tag="VHS97_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446827.1"
                     /translation="PGHERRNLAELNRMERKDSRTTVRQTMPPPEPRNGVKLGRPTPI
                     QDDTSMVPRVPAGAAARSRGATTSPLEALVTPPLPVSPDNSATQPASGTWAQANLFQI
                     ER"
BASE COUNT          526 a          708 c          694 g          490 t
ORIGIN      
        1 cgacaatctc caccggcagg tcgggaatcg aaccgacgat cacctcctca ccggcccgta
       61 acccttcctt gtgcgcgatc ccgcggtcgg ttcgggcgat gatcccgagt gcaccggtca
      121 actccatgag caatttcagg atcgagtccc gccgcgtgta gagcgccagg ctggtgaatt
      181 gcgccaccag ccagcgatca tagcgatcga cagtaaggcc cgagagaccg tccccttcgc
      241 tgaagatcag acggtatgcc gacgagtttt tgcctaggtc gagaatttcg ctccgcagcc
      301 gccctgccgc cgccagcgtc ttggaccaaa atgcgccgtc gagcggaccg tcgtcccaac
      361 gatagagcct tacgcggatc gtactcgtag ggttatagag tcccgtggcg atgcgttggc
      421 cgtcattggt gacgacgtca acctgatcgc cggcacgcgg ttcgccgatc acacggtcga
      481 tcgacgaatc aaaaacccac ggatggcgcg caaaaaacgg tcgcgcccgg cgcggcttca
      541 agatgacagg gccaaaagcg gtcgtcgagc ctgccaatga tccacctcaa tatcgcattc
      601 cagaccgggc gaaccgggtc gaaaggcctg cggtccggat atcaggatca gggatcggct
      661 ccgcgttgcc ggtcagggat tgcttgacga ctcgcgactg gtcgcttcga aatcggccag
      721 ataagccaga gtacggtcga gttgggtctt caaatgacgt acccgtaaca agctacgaat
      781 ccgacagagc agctcgacct tgttgacggg cttggtcaga aaatcatcgg tcccgacctg
      841 aacaccgcgt tcaaagtcgg cggcctcgtt gagcgcagtc accatgagta tcaaaaggtc
      901 tttggtatcg gcattggagc gcagcttttt gcaaacctca aagccagaca aacggggcat
      961 catcacgtca agtaagagca agtccggctt gaattcgtct acgaccaaaa gcgtctcttc
     1021 gccgtcgtgc gcggtccgga tctcgcaatc gaaatcgctg aggtaagctt ccagcagctc
     1081 gacgttctgg tagttgtcgt cggcgatgac gattttgggg gcaccgggag agtggtccaa
     1141 attccgttct cctcgaggaa ccaaacagtc gctcaattct aaccccgagt ttcttcacag
     1201 agaacctgaa tattcaacaa actctcgtga attcccctta aaatgctggg tcgatccaca
     1261 gtattctcgc gtaaggaacg gaaacaatca gaaaccacgg cgatcgccgc cgtcggaacc
     1321 tttcggttgc cgaacctcgt caggctcggc cgcgtattga caaaggcctc tcgggcaagg
     1381 ttgccaccgg tcatctctgt gatacaattg taaagtttca gacatgggat gagttccaag
     1441 gcggctcctc catcgtctcg agccttcttc tttgagaggg ggcaacgccg tgcccttgcg
     1501 tgatattgac cgcaagctgg ttgatcgctg cctgcagaaa gagcccggcg cctggaacga
     1561 ttttgtcgat cgttacatgg ggctgatcta ccacgtgatt caacacgtgg cgtacgcgcg
     1621 aagccgtctt ctctcgtcgg aagatgtcga agacatcggc gctgagatcc tcctgaaaat
     1681 tgtcgacaac gactatgccg tcctcaagaa gttcaagggg atcagctcac ttcctacata
     1741 tctaacagtg atcgcacgta ggacttgcgt taaagagctt atcaagcggc atcgcgagga
     1801 agagctgagc cacacaagcg cccaccgcgc atttacggac gacggtgtct cgggcgaagt
     1861 cgaggcgatc ctctcggccg aggaagtggg tcggatgctc gaagatctga gcgaccgcga
     1921 agccgaggtc attcggcttt atcacctcaa gtttttgaac tatcgccaga tcggcaagca
     1981 gctcgggcta cctgagaatt cgatcggtcc gatcctggcg aaggcccgcg ccaaacttcg
     2041 acacgcggcc gagaagcagc aaagcgcaag ttgatcaatg ggtcgcaacg acacaactgc
     2101 ctatcagcgt tcgatttgaa acaggttggc ttgggcccat gtgccgcttg cgggctgcgt
     2161 cgccgagtta tcgggggaga cgggcaacgg cggggtcaca agtgcctcca agggcgatgt
     2221 tgtcgcgcca cgcgaccgcg cggccgcacc ggcgggaacg cggggcacca tcgatgtgtc
     2281 atcctggatc ggtgtcggcc gcccgagttt cacaccgttc ctgggttccg gcggcggcat
     2341 agtctgtcgc accgtcgtcc gcgaatcctt ccgctccatt cgattgagtt ccgccaagtt
     2401 tctccgttca tggccggg
//
LOCUS       DATAQL010000070         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3824026, whole genome shotgun sequence.
ACCESSION   DATAQL010000070 DATAQL010000000
VERSION     DATAQL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3824026"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1539
                     /locus_tag="VHS97_01165"
     CDS             <1..>1539
                     /locus_tag="VHS97_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446828.1"
                     /translation="GDAWLAYVQFHHSPDHVKLAAPLHEAPKDFSSLKQPVGGDQIWL
                     QKYSGGAWQQPVAVTKAGKDQYRAAVAVDGSGRPWVFWSENRGGNFDIFVSPVDQSGP
                     QSEIQISKESGSDIDPVAVTDSNGKVWVAWQGWRDGRAAIFTAHQNGNSFTAPEKIST
                     STKNEWDPAIAADKAGRVAVAWDSYRNGNYDVYARTWSNSWAAEVPIAASARYEAYPS
                     IAFDPKGRLWVAYEEGGEGWGQDFGAYASPGIALYKGRAIRLRALEPDGRLVTIQSSI
                     DKTLIGIPSARPDILGSQLDGEPRDPDTQSALTRKPDQAPEGGAQTTAKNTLPRLTID
                     DSGRISIAFRTAHPLWWSPIGTVWSEYVISFDGKQWSKPIYLDHSDNLLDNRPAVLAT
                     APGKLLIVNSSDGRHKFVPASSIMGEREGTKDPYNNDLWSQEIDLGPGDQVLPVVALS
                     QSEKTPSAPTWESVEVNGAKAIREYKAGPDGSYHIVRGEFHRHSEISMDGGGDGTLLD
                     QWRYV"
BASE COUNT          330 a          470 c          459 g          280 t
ORIGIN      
        1 ggcgacgcct ggctcgccta tgtgcagttc caccatagtc ccgatcacgt gaagttagcc
       61 gcgccgctgc atgaggctcc caaggacttc tcgtcattaa aacaaccggt gggcggagat
      121 cagatctggc tgcagaaata ctcaggcgga gcgtggcaac agcccgtcgc cgtaaccaaa
      181 gccgggaaag atcaataccg ggccgcagtt gcggtcgatg gaagcggccg gccatgggtc
      241 ttttggtcgg aaaaccgagg cggaaatttc gacatctttg tcagccccgt cgaccaatcc
      301 ggaccgcaga gcgagattca gatttccaaa gagagcggat ctgatattga tcccgtcgct
      361 gtgaccgatt cgaatggcaa agtttgggtt gcctggcagg ggtggcgcga tggccgggcg
      421 gcgatcttta ccgcgcacca gaacggaaac agctttacgg cgccagaaaa gatttccacc
      481 tcgacaaaaa atgagtggga cccggccatc gcggccgaca aggcggggcg ggtggccgtg
      541 gcttgggact cctaccgcaa cggcaactat gacgtttatg cgcgcacctg gtcgaactcc
      601 tgggcggcgg aagttcccat cgctgcctcg gcccgctatg aagcctatcc ctcgatcgcc
      661 ttcgacccca agggccgtct atgggtagcc tacgaagagg gcggcgaagg atggggccag
      721 gacttcggcg cttatgcgag ccctggcatt gcgctctata aaggccgcgc cattcggctg
      781 cgcgcgctcg agcccgatgg acggctggtc accattcagt catcgatcga caagactctc
      841 atcggtatcc cctctgcgcg ccctgatatc ctcggcagcc agctcgatgg agaaccgcgc
      901 gacccggata cccagagcgc acttacccgc aagcccgacc aagcccccga agggggcgcc
      961 cagacaacgg cgaagaatac tctgccgcgc ctgaccatcg acgactcggg acggatctcc
     1021 attgcctttc gcaccgcaca tcccctctgg tggagtccca ttgggacagt ttggtcggaa
     1081 tacgtgatct cgtttgatgg caagcagtgg tcaaagccca tctatctcga tcattccgac
     1141 aacctgctcg acaatcgtcc cgcggtgctg gccaccgctc cgggaaagct gctgatcgtc
     1201 aactcctcgg acggccgcca caaatttgtt cctgccagct ccatcatggg cgaacgtgaa
     1261 ggaacgaagg atccctacaa caacgaccta tggagccagg aaatcgatct tggtcccggc
     1321 gaccaggttc ttccggtggt cgcgctttcg cagtcggaga agacaccctc cgccccgact
     1381 tgggaaagcg tggaggtcaa cggggcaaaa gcgattcgcg aatataaggc aggacccgac
     1441 ggctcgtacc acatcgtgcg cggcgaattt catcgccata gcgagatatc gatggatggc
     1501 ggcggggacg gcacgctgct tgaccaatgg cgctatgtg
//
LOCUS       DATAQL010000071         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3213435, whole genome shotgun sequence.
ACCESSION   DATAQL010000071 DATAQL010000000
VERSION     DATAQL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3213435"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VHS97_01170"
     CDS             complement(<1..1806)
                     /locus_tag="VHS97_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3446829.1"
                     /translation="MAAVLLAISFGLCAGYLDLFLMLFKKFYWKDEWILRIGRDFPWT
                     VPVAHAVLLLIPGMAIGAASRLRPRLVSMRAGSWLFATLAIWSALLRLPLYGACSLML
                     AAGLGRQIGDAVASRCWSSRSVGYSLAGLLSLLAVLAALSSGQQAIREQLAVAGLPPP
                     SPDAKNVVLIVWDTVRASNLSAYGYPRNTTPNLAQWARQGVRYSLAVAPAPWTYPSHS
                     CFFTGQWPFKLNSQWKFALDTAGPTFAEFLAARGYQTAGFSANTNCCNYETGLARGMA
                     HYEDFPLTPRSLVGRTVLGRWILANILYRGLYHDMKWIGLQSRGAHGTNEAFLGWLRG
                     RRPDRPFFAFLNYFDAHAPYVPPPGFVGRFGIRPRPPRDYDFLLGNMGAANNPTIARD
                     AQMARDCYDDCIAFLDDELGRLLDELRGQGLLDNTVVIITSDHGESFGDHGHFGHGSS
                     LHFDAIGVPLVILSPTAPAGLVVDSPVSLRDLPATVVDQLALSAGSPFPGRSLAAYWR
                     SKPGQVPRGITTPALSEQADAIAFEPQPQSGHGHSGFQMSLVASGHHYIRTGLGAEML
                     YDLVRDPSELINLIGSSYGDQTVGVFRNMLLEALSD"
     gene            complement(2124..>2864)
                     /locus_tag="VHS97_01175"
     CDS             complement(2124..>2864)
                     /locus_tag="VHS97_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3446830.1"
                     /translation="TVSNSIVNTFLCPSDDMAPIPVPANAQWSGRLNNYFASVGTTIA
                     YQGALDTTGCFTQAGRAYGVQNFTDGTSNTIAFAEALVGPDSGGYQTKTQQALFRNGT
                     NVSPSSSAGSSSLFDANTNYNAVMADMQTCQTAALTLPSGDGVQGTLNEDDKGERWAA
                     DDGGFTLINTVVPPSSTQFSFACCAFAQSYGCDDGSFENVNSRHPGGANVLFADGSVH
                     FLKSSIAIKTYWALGTKGNGEVVSSDSY"
BASE COUNT          592 a          834 c          922 g          516 t
ORIGIN      
        1 atcgctcagc gcctcaagga gcatgtttcg aaagactccc acggtttggt cgccgtagga
       61 cgaaccgatg aggttgatga gctcggacgg atctcgtaca agatcgtaga gcatctcggc
      121 tcccaaacca gtccgaatgt aatgatggcc cgaggccacg agtgacatct gaaacccgct
      181 gtgcccgtgg ccgctttgag gctgcggttc gaaagcgatc gcgtccgcct gctctgagag
      241 tgctggggtg gtgattccgc ggggcacctg cccgggttta gaccgccagt acgccgccaa
      301 cgagcggccc gggaacggtg agcctgccga gagcgccaac tggtcgacca cagttgcagg
      361 caggtcgcgc aagctcacag gactgtccac cactaggccc gccggggcgg tcggggaaag
      421 gatcaccagc gggacaccga tcgcgtcgaa gtggaggctg gagccgtgtc caaagtggcc
      481 atgatcgccg aacgattccc catggtccga agtgatgatc accacggtgt tgtcgagaag
      541 cccctggcct cgtagctcgt cgagcaaccg accgagttca tcgtcgagga aagcgatgca
      601 atcgtcgtag caatcgcggg ccatctgggc atcacgtgca atggtcgggt tgttggcagc
      661 ccccatgtta cctagcagga aatcgtaatc ccgcggaggc ctgggccgga ttccgaagcg
      721 tcctacaaat cccggcgggg gcacataagg cgcgtgggcg tcgaaatagt tcaagaaggc
      781 gaagaagggt cgatccggcc gccgcccgcg cagccagccg agaaaggctt cgttggtccc
      841 gtgcgcgccg cgggactgga ggccgatcca cttcatgtca tgatagaggc cacgatacag
      901 gatgtttgcg aggatccacc tcccgagcac cgtgcggccg acgagggacc gcggagtcag
      961 cggaaaatct tcgtaatggg ccatgccgcg agccaacccc gtctcgtaat tgcagcagtt
     1021 ggtattcgct gagaacccag cggtctgata gccccgtgca gccaggaatt cagcaaaggt
     1081 tggaccggcg gtatcgagcg cgaacttcca ttgcgaattg agcttgaatg gccactgccc
     1141 ggtgaaaaag cagctatgcg aagggtacgt ccacggggca ggcgccacgg cgaggctata
     1201 cctgacaccc tgccgcgccc actgcgccag gttgggcgtg gtattacgcg gatagccgta
     1261 ggcgctcagg ttggaggcgc ggactgtgtc ccagacgatc agcacgacgt tcttggcatc
     1321 cggggatggt ggcggcaaac cggccacagc gagctgttcg cggattgcct gctgacccga
     1381 tgagagcgcc gccagaacgg ccagcaggct caggagaccc gccagactgt atcccaccga
     1441 ccgcgaggac cagcaacggc tcgcgaccgc gtcgccgatc tgccgaccca gcccggcggc
     1501 cagcatcagg ctgcaggcgc cgtacagcgg tagccggagc agggccgacc agatcgcgag
     1561 cgttgcgaac agccacgacc ctgcgcgcat cgagacgagg cgcggccgaa ggcggctcgc
     1621 ggcaccgatc gccatcccgg ggatcaagag cagtaccgcg tgggcgaccg ggacagtcca
     1681 ggggaaatcc ctgccgattc gaaggatcca ctcatccttc cagtagaatt tcttgaagag
     1741 catgagaaac agatcgagat agcctgcgca gagtccaaac gagattgcca gcagaaccgc
     1801 ggccatcggc gtcagtggaa tccggatcgg cggacgccga ggcgtaggtt tgggaggggg
     1861 gacattcgtc tggacttgcc cttccgcgct atccttgatg gttgtcacag tagaagtgac
     1921 ccgaaccttc gcccgagtgc tccggctaca cgccaatcat aggattgtat tggtgcactc
     1981 ggaacaaccg aggaggccgt catccataca aacgagttac agactccacg attgtaatcg
     2041 tcgttgttgt gagttacgag cccacgcgat caaagacggt gaagcgagat cgccattggc
     2101 tcgtcgaacc gggccgatcc cagtcagtag ctatcggacg agacgacctc gccgtttcct
     2161 ttggtcccga gcgcccagta cgtcttaatt gcaattgacg atttgaggaa gtgcacgctg
     2221 ccgtcggcga acaggacgtt ggccccgcct gggtgcctgc tgttgacgtt ttcgaacgat
     2281 ccgtcgtcgc aaccgtagga ttgggcgaat gcacagcagg caaaggaaaa ttgagtcgag
     2341 ctcggtggca cgaccgtatt gatgagtgtg aatccgccat cgtccgccgc ccaccgttcc
     2401 cccttgtcgt cttcgttgag agtaccctga accccgtcac cggaggggag ggtcagggca
     2461 gccgtttgac acgtctgcat atcggccatg acggcattgt aattggtgtt ggcgtcgaag
     2521 aggctggacg aacccgcact cgaggacggt gaaacgttgg tgccgttccg gaaaagcgct
     2581 tgctgtgttt tggtctgata gcctccagag tccggtccga ccagcgcttc ggcgaatgcg
     2641 atcgtgttcg aggttccgtc agtgaagttc tggacgccgt acgctctacc cgcctgggta
     2701 aagcatccgg tggtatcgag tgccccctga taagcgattg tcgtgccgac cgaggcgaag
     2761 tagttattga gtctgccgct ccactgggcg ttggctggga ccgggatcgg ggccatgtcg
     2821 tcggacgggc aaaggaacgt gttgacaatc gaattgctga ccgt
//
LOCUS       DATAQL010000072         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_770251, whole genome shotgun sequence.
ACCESSION   DATAQL010000072 DATAQL010000000
VERSION     DATAQL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770251"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VHS97_01180"
     CDS             <1..1110
                     /locus_tag="VHS97_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3446831.1"
                     /translation="QQIVGQNFKFFTVFAAAGIIYLVMTSAVAVGQAVLERRFNLEID
                     RTAGRGSGFANLFGFALPRLAKKAEAETPCDLKPTRRALAAMKSAEAASLDWIGNITA
                     PPGRADDQQPFVTLANVHKAYGSKEVLRGIDFSVNRGEVVVIMGPSGSGKSTMLRLVN
                     HLETLDWGEIKVEGKHVGYRMRNHNVLKPVRDLAKARAEARIGMVFQHFNLFEHMTAL
                     ENVMEAPIHVYGEPRDKVRRLAEELLATVGLRQHADKLPHRLSGGQQQRVAIARALAI
                     SPRLMLFDEPTSALDPELVGEVLAVMRKLAEAGMTMLVVTHEVRFAEEVADRVVFMDE
                     GLIVEQGTPAEVLGAPKQERTQRFLRMVDGEADVT"
     gene            complement(1162..1521)
                     /locus_tag="VHS97_01185"
     CDS             complement(1162..1521)
                     /locus_tag="VHS97_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446832.1"
                     /translation="MLGADNAEAFVERFWEPAFQHHDANDLLSLLRTWFEGDISANPL
                     YDGDYKRALSAIKARAIVMPCEYDRYFPPVDDHEAVSHMPNAQCRPIDSIWGHMCLWN
                     PTDQKVIDAALRELLEA"
     gene            1623..>2222
                     /locus_tag="VHS97_01190"
     CDS             1623..>2222
                     /locus_tag="VHS97_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446833.1"
                     /translation="MIGLLCFMLAVLASPFKSRLRLEAENAVLRHQLMVSRRRLRGRV
                     RITNHDRWFFVQLYRWFPSILSVLTVIRPETLVRWHRAGFRSYWRWKSRSSGGRPQID
                     PELRVLIRRMSMDNPPWGAPRIHGELLKLGFEIAQSSVAKYMVKRREPPSQGWRTFLH
                     NHAPEVAAMDFFVVPTIGFDLLYAFVIVRLDRRELVWINV"
BASE COUNT          397 a          684 c          709 g          432 t
ORIGIN      
        1 cagcagatcg tcggccagaa cttcaagttc ttcacggtct ttgcggccgc cgggatcatc
       61 tatctcgtga tgaccagcgc ggtcgcggtc ggccaagcgg tgctcgagcg gcggttcaac
      121 ctggaaatcg atcggacggc cggtcgaggc tcgggctttg ccaatctgtt cggcttcgcg
      181 cttccccgac tcgccaagaa ggcggaggcg gagaccccat gcgatctcaa gccaacgcgt
      241 cgggccctcg cggcgatgaa atctgccgaa gcggcctcac tcgattggat cggcaacatc
      301 acggcacccc ccggacgggc cgacgaccag cagcccttcg tcactctggc caacgtccac
      361 aaggcctatg gctcgaagga ggtgctgcgg gggatcgatt tctcggtcaa ccggggcgag
      421 gttgtggtca tcatggggcc gagcggatct ggcaagagca cgatgctgcg gctcgtcaat
      481 catctcgaga ccctcgactg gggtgagatc aaggtcgagg gcaagcatgt cggctaccgc
      541 atgcgcaatc acaacgtgct gaaaccggtc cgcgacctcg ccaaggcgcg agccgaggcg
      601 cgcatcggca tggttttcca gcacttcaac ctgttcgaac acatgaccgc gctggaaaat
      661 gtcatggaag caccgatcca cgtctacggc gagccgcgcg acaaggtgcg ccgcctcgcc
      721 gaagagctat tggcgaccgt gggcctgcgc cagcatgccg acaagctgcc gcatcgtctg
      781 tccggcggcc agcagcagcg cgtggccatc gcccgtgccc tcgccatttc acccaggctg
      841 atgctgttcg acgagccgac ctcggcgctc gatcctgagc tcgtgggcga ggtcctggcc
      901 gtgatgcgca agcttgccga ggccggcatg accatgctgg tggtgaccca cgaggtccgc
      961 tttgccgaag aggttgccga ccgggtcgtc ttcatggacg aagggctgat cgtggagcaa
     1021 ggcacgccgg cggaggtctt gggtgcgccc aagcaggagc gcacccagcg cttcctgcgc
     1081 atggtcgacg gcgaggccga tgtcacctga gccatgatga gcgaactggc ggccccgtcg
     1141 cccgaggccg cctgaatgct atcaagcctc gagcaattcg cgtagcgccg cgtcgatcac
     1201 cttttggtcc gtggggttcc agagacacat gtggccccag atcgaatcga tcggccggca
     1261 ttgcgcgttc ggcatatggc tcacggcttc gtggtcgtcg accggcggaa aatagcggtc
     1321 gtattcgcag ggcatcacga tcgcccgtgc cttgatggcg gaaagcgccc tcttgtagtc
     1381 gccgtcgtag agcggattgg cgctgatgtc gccctcgaac caggtcctga gcaggctaag
     1441 cagatcgttg gcgtcgtgat gttggaacgc cggttcccag aaccgttcca cgaaggcctc
     1501 tgcgttgtcg gcgcctaaca tccggtaggc ctcggtgcgg aaaaatggct ccgaaaatcc
     1561 cctgtgtgta ccgaaaactt tgtccgggtt gatgatgtga ccaaatcggc taagctggcg
     1621 ggatgatcgg gcttctctgt ttcatgctgg ccgtcctcgc ctcgccgttc aagtcgaggt
     1681 tgcggcttga agcagagaac gcggtgcttc ggcatcagtt gatggtctcg cggcggaggc
     1741 tgcgtggtcg tgtccggatc acgaaccatg atcgctggtt ctttgtccaa ctgtatcgct
     1801 ggtttccatc aatcctgtcg gtcctcaccg tgatccggcc cgagacgctc gtgcgttggc
     1861 acagggccgg ttttcgcagc tactggcgtt ggaagtcgcg atcttcggga ggccgaccgc
     1921 agatcgaccc ggaactgcgt gtgttgatcc ggcggatgag catggacaac cctccttggg
     1981 gagcgccacg catccatggc gaactgctca agctcgggtt tgagatcgcg cagtcgagcg
     2041 tcgccaagta catggtcaaa cgacgggagc cacccagcca aggatggcgg accttcttgc
     2101 acaaccacgc gccggaggtt gccgccatgg acttcttcgt cgtcccgacg atcggcttcg
     2161 acctgctcta tgccttcgtt atcgtccggc tagatcgcag agaactcgtc tggatcaacg
     2221 tc
//
LOCUS       DATAQL010000073         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3671633, whole genome shotgun sequence.
ACCESSION   DATAQL010000073 DATAQL010000000
VERSION     DATAQL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671633"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VHS97_01195"
     CDS             <1..464
                     /locus_tag="VHS97_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEX3446834.1"
                     /translation="GNALYVLVKPENAGADLFQPVVGRGTAYADIDGDGDLDVVVTVN
                     KAPARLFRNDGGSNNHWIRLELTGDGKMSNRDAIGAKIELKAGGNDCHRQLFPSKSYL
                     SSVEHTLTFGLGQLDQADTVKITWPSGKVSIFENLKAGKVYRVDEDGGLHQ"
     gene            721..>2059
                     /locus_tag="VHS97_01200"
     CDS             721..>2059
                     /locus_tag="VHS97_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3446835.1"
                     /translation="MLTIAGRARSGYCDGVTRRDFLRIGGLGLGGLSLSQLLRAEAQA
                     GIARSHKAVIMIFLAGGPPHMDMFDLKPDAPSDIRGEYAPIATNVPGLEICEHMPRLA
                     QMMDKFAVVRSLVGAGGDHSAGQCLTGYRDMVSKSQGGRPSLGAVVSLLQGPVHGDVP
                     PFVGLSPRTGELRWGNPGNPGYLGLAHAPFTPFRTETKGNRNSKQGPGDAGLSLDETA
                     IVPERLAGRRSLLAVLDNLRRKIDHDDAMLGLDSFNQRAFEVLSSRRVFEALNLSKED
                     PRLRARYGIGDMRNDDDGPPCCMDHFLMARRLVEAGVRVVTISFGRWDTHKQNFETCR
                     SRIPKLDMALSSLVQDLHVRGLDKDVSVVVWGEFGRTPKINNDAGRDHWPAVNFALLA
                     GGGMRTGQVIGSTDKEAAYVKERPVSFQNVFATLYHNLGIDPGLAVPDRSGRPM"
BASE COUNT          407 a          638 c          626 g          388 t
ORIGIN      
        1 ccggcaatgc gctctacgtc ctggtcaagc ccgagaacgc aggtgccgac ctgttccagc
       61 ccgtcgtggg gcgcggtacg gcctacgccg atatcgatgg cgacggcgat ctcgacgtcg
      121 ttgtcaccgt caacaaggct ccagcacggc tcttccgtaa cgacggtggc agcaacaacc
      181 actggattcg cctcgagctc accggcgacg gcaaaatgtc caatcgcgac gccatcggcg
      241 ccaagatcga gctgaaggcc ggtggcaacg actgccaccg ccagctcttc ccatcaaaaa
      301 gctacctttc atcggtcgag cacacgctaa ccttcggcct gggacagctc gaccaggccg
      361 ataccgtgaa aatcacctgg ccatccggca aggtctcgat tttcgagaat ctcaaggccg
      421 gcaaggtgta tcgcgtcgac gaagacggcg gattgcacca atagtcgcga acgcccaggc
      481 gatgcatttc gtaatgatta taaggggagt gatgtcggca aaagcgcccc gatgcacaaa
      541 agaatcattg ccgatatccc agaccacaaa ttctcccatt ccgtgcggag cgcgcccgga
      601 aaatgacgaa taataaacct actgccttgt cttcgcgttc gaccgtcgca ctctttttgt
      661 taaacgaagt gttaacaatt tttggcggtg ggacgcggag cggacgaggg aggtggccag
      721 atgctgacga tcgcgggtcg ggcgcgctcg ggctactgcg acggggttac ccgccgcgac
      781 tttctcagga ttggagggct gggcctgggg ggactgtctt tatcccagtt gctccgggcc
      841 gaggcacagg ctggaatcgc gcggtcgcac aaggccgtga tcatgatctt cctggccggg
      901 ggccctcccc acatggacat gttcgacttg aagccggacg ctccatccga catccggggc
      961 gaatacgctc ccatcgccac caatgtcccg gggctagaga tctgcgagca tatgccgcgg
     1021 ctggcgcaga tgatggataa gttcgcggtg gtccggtctc tcgtgggcgc cggcggtgat
     1081 cactcggcag gtcaatgctt gaccggttac cgcgatatgg tcagcaagtc acaggggggt
     1141 cggccaagcc tcggagccgt tgtttcgctg ctccaagggc cagtgcacgg cgacgtacca
     1201 ccctttgtgg gcttgtcgcc tcgaaccggc gaattacgct ggggtaatcc cggcaacccc
     1261 ggctatctcg gcctggctca tgccccattc acaccgttcc ggaccgagac caaaggaaac
     1321 cggaacagca aacagggccc tggtgacgca gggctgagtc ttgacgagac ggcgatcgtg
     1381 ccggagcggc tggccggccg tcgttccctg ttggccgtgc tcgataacct caggcgcaag
     1441 atcgatcacg acgacgcgat gctcgggttg gacagcttca accagcgtgc gtttgaagtc
     1501 ttgtcctcgc gacgcgtatt cgaggctctc aacctcagca aggaagaccc acgactccgg
     1561 gcccgctacg gcattgggga catgcgtaac gacgacgacg gtcccccctg ctgcatggac
     1621 cacttcctga tggcccgtcg cctggtcgag gccggcgtgc gggtcgtgac catttcattt
     1681 ggccgctggg acacacataa gcaaaacttc gagacttgcc gctcgcggat ccccaagctc
     1741 gatatggcgc tctcgtcact tgtgcaagac ctgcacgtgc gtgggctgga caaagacgtc
     1801 tccgttgtgg tctggggcga gtttggccgg acgccaaaga tcaacaatga cgccggccgc
     1861 gatcactggc cggcggtgaa tttcgccctc ttggccggcg gcggaatgcg tacgggccag
     1921 gtcatcggct cgaccgacaa ggaagccgcc tacgtcaagg agcgaccggt ttcatttcaa
     1981 aacgtgttcg cgacgcttta tcacaacctc ggcatcgatc cgggcctcgc agtccccgat
     2041 cgctccggcc ggccgatgt
//
LOCUS       DATAQL010000074         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_312611, whole genome shotgun sequence.
ACCESSION   DATAQL010000074 DATAQL010000000
VERSION     DATAQL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312611"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VHS97_01205"
     CDS             complement(<1..362)
                     /locus_tag="VHS97_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3446836.1"
                     /translation="MDPKKRVLIVDDESNVRLMLETALASVGYEVIEATDGHAALDLL
                     RGPDFHCDLVLLDLLMPRMDGMELLRLLRVSGNVVPVVILTAHGSIPEAVEAMKLGAI
                     DFLTKPTTPDALRGVVAEV"
     gene            complement(338..2224)
                     /locus_tag="VHS97_01210"
     CDS             complement(338..2224)
                     /locus_tag="VHS97_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3446837.1"
                     /translation="MKTLQTKILIGLLPTLAIVVGLGLWAIVMFYRLGGNIDVILREN
                     YTSILAAEGMKEAIERMDSGLLFAVGGRDEYGKTQFDEYRPRFLAHLKTEKANITLPG
                     EEGLAQSVDRLFQQYTESSARFFAQPHLPPERRTEIYFGELKPAFDKIKASADEILVL
                     NQKNMTAMDRRARDNASASIRLMIGVVIAAVVLAVAASLFLSRSILQPIQAVTDGARA
                     LARGELDQLVPAASRDELGELANAFNEMARTLREYRQAGTARLLRAQRTAQATIDSFP
                     DPVVVVDTVGSVEQANRAGRRLMGASDTAELSVPWSPVQPLNTLVNDVLAGRGDYLPL
                     SLEQAIPLSDGEQERYYLPRVVAIRTGHNELIGAAVALVDVTKFHLLDRLKSDMVATV
                     SHELKTPLTSVQMAIHLLLEELIGPLNPKQVELLIAARQDADRILTMINDLLDLTRIE
                     QGRVRLDLEPLSAALLVEEALHRFQSQAQASGVALAADIKGDTLTVMVDRDRIEHVFD
                     NLIHNAIQHSNRHGNIRVSAIPDGDQAKFVVEDNGEGIASEHVAHIFEKFYRVPTGRH
                     AGGAGLGLAIVREIITAHGGEIGVVSKPGRGTTFTFTLPAVGSADESKSIDGAKIWTQ
                     RSAS"
     gene            complement(2529..>3598)
                     /locus_tag="VHS97_01215"
     CDS             complement(2529..>3598)
                     /locus_tag="VHS97_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446838.1"
                     /translation="FDAIDGRDVALVFLASFVLKGDKGQWYWEIAKEMTDSDLREFLQ
                     NAADREFELLKPDNASSAREALLGIIERATERLTLKAHAHRERAEVKAELAADILAFD
                     DSPEGECLRRYELAHGRGVARAIDLRRKHRPTANQIDRNVATGTVAEFDILQQAHAIT
                     VENATIEPTAAHENVTSEPNITCENATIEPTLAADVGLESPTYIKALEHNVTIEPNDV
                     GENLTNEPPFAGHGSDRESAELRTLADGSDDEHFHAEVDIEKASKWIREAVEKRQAIR
                     ADELRRLNEESRTEADAVAVVRRTCRRGMGIEQSESTPHVERKAGGIGKTSEEKMHPA
                     LHRLLTGRESTLLDMSPILGK"
BASE COUNT          641 a         1109 c         1065 g          783 t
ORIGIN      
        1 acctcggcga ccacaccccg cagcgcgtcg ggagtggtcg gtttcgtgag aaaatcgatc
       61 gcgcccagct tcatggcctc gactgcttcg ggaatgcttc cgtgggcggt caggatcacg
      121 accgggacga cgtttcctga gacgcgcagc aggcgaagca gctccattcc gtccatacga
      181 ggcatgagga ggtcgagcag aacaaggtcg caatggaaat caggtccgcg cagcagatcg
      241 agggcggcat gtccgtcagt tgcttcaata acctcatagc cgaccgacgc cagcgccgtc
      301 tcgagcatga gccggacgtt cgattcgtcg tcaacgatca agacgcgctt ctttgggtcc
      361 atatctttgc gccatcgatg gattttgact cgtctgccga gccgaccgcc ggcagggtga
      421 atgtgaacgt cgtacctcgg cccggcttgc tgacgacacc gatctcaccc ccgtgggccg
      481 tgatgatctc gcggacgatc gcgaggccca gaccggctcc gccggcatgc cgcccagtcg
      541 ggacgcggta aaacttttcg aaaatatgag ccacgtgctc ggaggcgatc ccctcgccgt
      601 tatcttcaac cacgaatttg gcctggtcgc cgtcaggaat cgcgctcacc cggatatttc
      661 cgtgcctgtt cgagtgctgg atcgcgttat ggatcaggtt gtcaaagacg tgctcgatcc
      721 gatcgcgatc gaccatgact gtcagggtgt ctcccttgat atccgccgcg agggctacac
      781 cgctggcttg tgcctgcgac tggaatcgat gaagcgcttc ctcgacgagc agggcggcgg
      841 atagtggctc gagatcgagc cggacacgcc cctgttcgat ccgggtgaga tcgaggagat
      901 cgttgatcat agtgaggatt cgatcggcgt cttggcgcgc tgcaatgagc agctcgactt
      961 gcttgggatt gagcggccca atcagttcct cgagcaggag gtgaatcgcc atctgcacgc
     1021 tggtgagcgg agtcttgagc tcgtggctga cagtggcgac catgtcgctc ttgagccgat
     1081 cgagcaaatg aaattttgtc acgtcgacaa gggcgacggc ggctccaatc agctcgttgt
     1141 gcccggtgcg gatcgccacg acccggggca gatagtagcg ctcctgttcg ccgtcggaca
     1201 aaggaatggc ctgttcgagc gatagcggca ggtaatcgcc gcgacctgcg agcacgtcgt
     1261 tgaccagagt attaagaggt tggactggtg accagggcac ggagagttcg gcagtgtcgc
     1321 ttgcccccat gagccgccgg cccgcgcggt tggcctgttc caccgagccc accgtgtcga
     1381 ccaccacgac cgggtcggga aatgagtcga tcgtggcctg ggcggtcctt tgcgcccgca
     1441 gcagacgggc ggttccggcc tggcgatact cgcgcagggt ccgggccatc tcgttgaagg
     1501 cgttggccag ctctccgagc tcgtcgcgcg aggcggccgg cacgagttga tcgagctctc
     1561 cccgcgccaa ggcgcgcgcg ccgtcggtca cggcctgaat cggttgcaga atcgatcggc
     1621 tcaagaacaa ggacgcagcc accgccagta ccacggccgc gatcaccacg ccgatcatca
     1681 gccggatcga cgccgacgcg ttatcgcgcg cgcggcggtc cattgccgtc atattcttct
     1741 ggttcagcac cagaatctcg tcagcgctcg ccttgatctt gtcaaaagcc ggcttcagct
     1801 cgccgaaata gatttcggtc cggcgttctg gtggcaggtg gggctgggca aaaaaccggg
     1861 cggagctctc ggtgtactgc tggaaaagac ggtcaaccga ctgcgccaga ccctcttccc
     1921 cgggtaacgt gatgtttgct ttctccgttt tcagatgtgc caggaaccgc gggcgatatt
     1981 cgtcgaactg cgttttgccg tactcgtctc gaccgccgac ggcgaacagc aggcccgagt
     2041 ccatgcgctc gatcgcttcc ttcatcccct cggccgcgag aatgctggtg tagttttcgc
     2101 gcaaaatgac atcgatgtta ccccccaagc gataaaacat gacgatcgcc cagagcccga
     2161 gccctaccac gatcgccagc gtcggcagca acccgatcaa aatcttggtt tggagcgtct
     2221 tcacctgacc tacccttcag caaacgctga gcgattcccg tgccgcatcg ggcattgccg
     2281 ccgtaagtcg ctcgtcatca tacgcaaagg gttcagagga atcttttgcg gcgtcacgga
     2341 cgtgccgcga cggcgttgca atttgcacga taatcccacc gatagctcgc aaattgcaat
     2401 ggtgcgatgt cgcacgacca agactgagag caggacctct tgaccgtgcc gcacgtggcc
     2461 gcctttgcgc gcaccatcaa cccgtgcgtt ctcttttctc atagtgtcta ctgcgtagtg
     2521 ccccctgctt actttccgag aatcggcgac atgtccaaaa gagtcgactc ccggcccgtc
     2581 aggagccggt gaagagcggg gtgcatcttc tcttcgctgg ttttcccgat tccgccagct
     2641 ttgcgttcaa cgtgcggcgt tgattctgat tgctctatcc ccatccccct gcggcaagta
     2701 cggcgcacta ctgcgactgc atccgcctct gtacgactct cctcattcaa tctccgcagc
     2761 tcgtctgccc ggatcgcctg tcgcttctcg actgcttccc tgatccactt gcttgccttc
     2821 tcgatgtcaa cctcggcatg gaagtgctcg tcgtcactgc catcagcaag ggttcttagc
     2881 tccgcgctct cgcggtccga gccgtgaccg gcgaacgggg gttcgttcgt caagttttcc
     2941 cccacgtcgt tgggttcgat cgtcacatta tgctcgagtg cttttatgta ggtcgggctt
     3001 tctagcccga cgtcggcggc aagtgtgggt tcgatcgtcg cgttttcgca ggtaatgttg
     3061 ggttcgctcg tcacgttttc atgggcggcg gtgggttcga tcgtcgcgtt ttccaccgtg
     3121 atcgcgtggg cttgttgcag gatgtcgaat tcagcgactg tgccggtcgc gacatttcga
     3181 tcgatttggt tagctgtggg gcggtgcttt cgccgtaagt cgatcgcgcg ggccacgccc
     3241 cgaccgtgtg ccagctcgta acgacggagg cactcgcctt ccgggctgtc atcaaaagcc
     3301 aggatgtctg cggccagctc tgccttcact tcagctcgct ctcgatgggc gtgggccttc
     3361 aacgtcaagc gctcggtggc tcgctcgata atccccagca gtgcctcccg ggcactgctg
     3421 gcattatcgg gtttgagtaa ttcgaactca cgatccgcgg cattctgcag gaattctctt
     3481 aaatcggaat cggtcatttc cttcgcgatt tcccagtacc actggccctt gtcgcccttg
     3541 agcacaaacg aggccagaaa cacgagtgcc acgtccctgc cgtcgatggc gtcaaagg
//
LOCUS       DATAQL010000075         4955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3671783, whole genome shotgun sequence.
ACCESSION   DATAQL010000075 DATAQL010000000
VERSION     DATAQL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671783"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VHS97_01220"
     CDS             <1..1054
                     /locus_tag="VHS97_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3446839.1"
                     /translation="VKFQSEVDRWDVEVKRLERQVSGTVVNTSVLFESTNQLKSSIAA
                     LDKAKATIKRAKAELIAAEARHAKAQVDVRVMEADLKVADSQEKFAQAWVGYLTLTAP
                     FTGVVTARNANTFDFVLPATGDPTAFYLSPDISTAGAAPIYVVDRLDIVRIFVDIPEQ
                     DANFVNIGTKATVLAKAFRDKELPATVTRVSWALNQKSRTLRAEIDLENPDSQLLPGM
                     FAYVKVIIERPDVQALPLDALVYDGDQTFCWLYKNGKAVRAELETGVSDDKWIEVTNR
                     RPPVDQEAPGDMVPWTRIDGTEQVILGDFSGLADNAPVEVATGERAAKLAGAVPSLDY
                     HPAKTTAGTGTVETSK"
     gene            1051..2625
                     /locus_tag="VHS97_01225"
     CDS             1051..2625
                     /locus_tag="VHS97_01225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3446840.1"
                     /translation="MIPRVNLSTVSDRPVRCRGRVLWPPFGLWAALVVTGCHHEAENH
                     YTSVGKPPTVQLVQPQIRDIVRVIGQPSFTQSYERSSVYPKMNAYIQKWIVDIGDKVK
                     KGDVLATLFVPELVEDHGTKKATVVLDQEKIALAKVVVEVAEAEVEAALARLDEARAD
                     FASNKAEADRWDAETKRLENELKRGVVNPQDVLQASNRWKAMVASRETANASVLKAEA
                     ELLARRAALAKAKVDVRVAEADLKVAESEEKRLQAWVDYLVLPAPYDGVIVARNANTF
                     DFVLPTTGDPSALSRGPYMSASGSAAPIYVVDRTDIVRVFVDIPEQDANYVQIGSKAT
                     VLVRAFREEPIPGTVTRTSWALNVKSRTLRAEIDLPNPGSKLLPGMYAYTKVMIERHK
                     VRTLPVSALMHLGDKTVLQVGETSFCWIYNDGRAKRIEVETGVSDGEWIEITNRRAVT
                     SQGEEPWTPFDGTEQVILGNLSILVDNGPVQIAPETDTSKANGAAAAANRVVRPDASR
                     AIQRVAGGGPVPTGGR"
     gene            complement(2580..4466)
                     /locus_tag="VHS97_01230"
     CDS             complement(2580..4466)
                     /locus_tag="VHS97_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3446841.1"
                     /translation="MDRIGRITVVVATLLVVGFEPWPCHAQRAALPATSIPPPRPGRD
                     VGAMERKLAALEPTDLRSPILVSPALEDLLEAAAREPIDPCLSVNFATAFRPPSESTA
                     PTAPRRIIPPPRPFGQQSPLTASSSSPPADPAVVVATGDLVARLKRAPLESTDVGFPI
                     NLATALRLSDARPLIVAAAQASVWVAEAQLTRAKVLWVPTLTLGGDYIRHDGGGPDFN
                     KGLLTAPSVNYFYGGGGLNVSNSGLFQFVNLTDAFFEPLIAQRVLNSRQWDIQSAKND
                     ALLLTADTYFRVHQHRGMYAGALYCVERGRNLVEQIASLSDELVSKFEVERARNLLAD
                     LEQQAVSARQAWRVQSANLTRVLRLNPQAVVVPLEHDHLQLTLIDPAQSLPDLQKIAL
                     TNRPELASQRELVQAAAERIRREKLRPLLPIVIIGGFQNAGGMMTQAGIFGLGPNTSL
                     NQWAGRDDVSLQLVWQFDAFGIGNLARIKEQRGGQSETFVSLYKIQDKVVAEVTEAQA
                     NLQSAAARVRQADRSLRTAIIAYNGNVEGLRNTTRYGDVLVLIYRPQEVVYALRLMKL
                     AFDEYFATVAEYNRAQFELFHALGYPANVITYLRSPGNIEPVDTERPAYLPPVGTGPP
                     PATR"
     gene            4705..4926
                     /locus_tag="VHS97_01235"
     CDS             4705..4926
                     /locus_tag="VHS97_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446842.1"
                     /translation="MPTFTIEYRDERHRIGLEQAIAYVTELHQIAQDAPRGSVLDACK
                     KLAKSDGRAEFVRESDARSRIQNPIFLNL"
BASE COUNT         1000 a         1501 c         1575 g          879 t
ORIGIN      
        1 ggtgaagttc cagtccgagg tcgaccgctg ggacgtggag gtcaaacgac tcgagcgcca
       61 agtctcgggg accgtggtga acaccagcgt ccttttcgag tcgaccaatc agctcaagtc
      121 gagcatagcg gcgctcgaca aggcaaaggc gaccatcaag cgggcgaagg cggaattaat
      181 cgccgccgaa gctagacatg caaaggccca ggtggacgtc cgagttatgg aagccgactt
      241 gaaggtggcc gacagccagg agaaatttgc gcaggcctgg gtcggctacc ttacgctcac
      301 ggcacctttc accggcgtcg tcaccgcccg caacgccaac acgttcgatt ttgtcctgcc
      361 cgccaccggc gaccccacgg cattttatct gtcgccggac atttcgaccg cgggggccgc
      421 gccgatctat gtggtcgacc gtctcgacat tgtccggatc tttgtcgaca tcccggaaca
      481 ggatgccaac ttcgtcaaca tcggcacaaa ggcgaccgtg ctggccaagg cgttccgcga
      541 caaggagctg ccggccacgg tcacgcgcgt ttcctgggcg ctcaaccaga agagtcgcac
      601 gctccgcgcc gagatcgacc ttgagaaccc cgatagccag ctcttgcccg gcatgttcgc
      661 ctacgtcaag gtgatcatcg aacgtccgga cgtccaggca ctgccgcttg acgcgctggt
      721 ttatgacggc gaccagacct tctgttggct ctataaaaac ggcaaggccg tgcgggccga
      781 actcgaaacg ggcgtcagtg acgataagtg gatcgaggtc accaatcgcc gcccccctgt
      841 cgaccaggag gcgcccggcg acatggtccc ctggacgcgg atcgatggca cggaacaggt
      901 gatcctcggc gatttttcag gcctcgccga taacgcgcca gtggaggtcg cgacagggga
      961 aagggcggcg aaactcgcgg gcgcagtgcc ctcactcgat tatcatccgg cgaagaccac
     1021 cgccgggacc ggaaccgtgg agacgtcgaa gtgattcccc gcgtcaacct atccaccgtc
     1081 tcggatcgac cggtccgctg ccgcggccgt gtgctctggc ctccctttgg tctctgggcc
     1141 gcgctggtag tgacagggtg ccaccacgag gccgaaaacc attatacgag cgtcggaaaa
     1201 cctccgaccg tgcagctagt ccagccgcag atccgcgaca ttgtccgcgt tatcggccag
     1261 cccagcttca ctcagagcta tgagcgatca tcggtttatc cgaagatgaa cgcctacatc
     1321 cagaagtgga tcgtcgacat cggcgacaag gtaaagaagg gcgatgtgct cgcgacgctc
     1381 ttcgttcccg agttggtcga ggatcacggt acgaagaagg cgacggtcgt gctcgaccag
     1441 gagaagatcg cactcgcgaa agtagtggtg gaggttgccg aggccgaggt cgaggcggcg
     1501 ttggcacggc tcgacgaggc ccgcgcggat ttcgccagca ataaggccga ggccgaccgc
     1561 tgggacgcgg agaccaagcg actcgagaac gagctcaagc gaggcgtggt caatccccag
     1621 gatgtgctcc aggccagcaa tcggtggaaa gcgatggtcg cgtcgcgaga aactgcgaac
     1681 gcaagcgtct tgaaggccga ggccgagctg ctcgcgcggc gggcggcgct cgccaaggcc
     1741 aaggttgacg tccgggtcgc cgaagccgac ctcaaagtag ccgagagcga ggaaaagcgg
     1801 ctccaggcct gggtggatta cctcgtactg cccgcgccct atgacggcgt gatcgtcgcc
     1861 cgcaacgcca atacctttga ttttgtcctg cccactacgg gcgatccctc ggcgctctcc
     1921 cgtggtccgt acatgtcagc ctcgggcagt gccgctccga tctatgtggt cgatcgcacc
     1981 gacatcgtcc gcgtcttcgt cgacattccc gaacaagacg ccaactatgt gcaaatcggc
     2041 tccaaggcca ctgtgctcgt ccgggcgttc cgcgaggagc cgattcccgg aaccgtgacg
     2101 cgcacttcgt gggcgctcaa cgtcaagagc cgcacgctcc gcgccgagat cgacctaccc
     2161 aaccccggaa gcaaactctt gcctggcatg tacgcgtaca ccaaggtgat gatcgagcgg
     2221 cacaaggtgc ggacgctgcc ggtgtcggcg ctcatgcacc tgggggacaa gaccgtcctg
     2281 caagttggcg aaacgtcgtt ctgctggatt tataacgacg gccgcgcgaa acggatcgag
     2341 gtggagaccg gggtgagcga cggcgagtgg atcgagataa ccaatcgtcg cgccgtcacg
     2401 tctcagggcg aagaaccctg gacgccgttc gacggcacag agcaagtgat cctgggcaac
     2461 ctatcgatcc tcgtcgataa tggtcccgtg cagatcgcgc cagagaccga cacttcgaaa
     2521 gccaatggtg cggccgcggc tgcgaatcgc gtggtccgac ctgatgcgag ccgggcgatt
     2581 cagcgggtcg ccggaggcgg tccggtgccc accgggggca ggtaggccgg tcgttccgtg
     2641 tccactggct cgatgttgcc cggggaccgg agataggtga tcacgttagc ggggtaaccc
     2701 agcgcgtgga atagctcgaa ctgcgcacga ttgtattcgg caacggtggc gaagtactcg
     2761 tcgaaggcga gtttcatcaa cctgagtgca taaaccactt cctgcggtcg atagatcaag
     2821 accaagacgt cgccatagcg agtggtattg cgcaacccct cgacgttacc gttataggcg
     2881 atgatggccg tgcgcaacga gcgatccgcc tgcctgaccc gcgccgccgc tgactggaga
     2941 ttcgcctgcg cctcggtcac ttccgccacc accttgtctt ggatcttgta aagactgacg
     3001 aaggtctcag actgcccacc gcgctgttct ttaatccgcg cgaggttgcc gatcccaaat
     3061 gcatcaaact gccacacaag ctgaaggctg acgtcgtcac ggccggccca ctgattgagg
     3121 ctggtgttgg ggcccaggcc gaagatccct gcttgcgtca tcatgccgcc ggcgttttgg
     3181 aagccaccga tgatgacgat cggcagaagc gggcgcagct tctcgcggcg gatccgctcg
     3241 gccgccgcct ggaccagttc gcgctgcgac gcaagctcgg gtctgttagt cagcgcgatt
     3301 ttctgaaggt cggggagcga ttgcgcgggg tcaatcaagg tgagctgaag atgatcgtgt
     3361 tcgagcggca cgaccaccgc ttgggggttg agccgaagaa ctcgggtgag gttggcactc
     3421 tgcacgcgcc acgcctgacg cgcggacacc gcctgctgct cgaggtcggc gagcaggttg
     3481 cgggcccgct cgacctcgaa tttcgatacc agctcgtcgc tcaagctggc gatctgctcg
     3541 accaggttgc ggccgcgctc gacgcagtag agcgctccgg catacatgcc ccggtgctgg
     3601 tggacgcgga aataggtatc ggcggtcaag agcagggcgt cgttcttggc ggactggatg
     3661 tcccattgcc gggaattgag aactcgctgt gcgatcagcg gctcaaagaa agcatcggtc
     3721 aaattcacaa actggaataa acctgaattg gacacgttta agccgccacc gccgtagaaa
     3781 tagttcacgc ttggggcggt caacaggccc ttattgaaat ccgggccacc gccgtcgtgt
     3841 cggatataat cgcctcctaa ggtcaatgtt gggacccaga ggaccttggc ccgcgtgagc
     3901 tgtgcttcgg ccacccagac actggcctgg gcagcggcca caatcagcgg gcgggcgtcg
     3961 gaaagccgca aggccgtggc gagattgatc ggaaagccga cgtcggtcga ctccagcggc
     4021 gcgcgcttga gcctcgcgac cagatcgccc gtcgctacca ccacggccgg atccgccggc
     4081 ggggacgacg acgacgcggt caatggactt tgttgtccga acggtcgcgg cggcggaatg
     4141 attctacggg gcgccgtcgg cgccgtcgat tcgctgggcg gtcgaaaagc agtggcgaaa
     4201 ttgacagaca agcagggatc gatcggctct cgtgccgcgg cttcgagcag gtcttcgagc
     4261 gccggagata cgaggattgg tgagcgaagg tcagtcggct cgagcgccgc gagcttccgc
     4321 tccatggccc cgacgtcgcg tcccggccgc ggaggcggga tcgacgtcgc tggtagggcc
     4381 gctcgctggg cgtggcaggg ccacggctcg aacccaacga caagcagggt cgcgacgacg
     4441 accgtgattc tgccgattcg atccatcagg cgcggctcct ccccaccctc ctgatcggaa
     4501 cctgcagcgc agccactgag gcctgacagg acgtcagaga ctttatcggc tcatgacaat
     4561 cctgatgttg atggcgaatg cagcgattct gagcccgcca cgttggaggg tgctcctctg
     4621 cattttggat gcgtcatgga tgacattgga gttcggtggt ttgcgcagaa caagagggct
     4681 tcaatagagc aaggaggctc gctaatgcca acgtttacga tcgaatatcg cgacgagcga
     4741 catcgcattg ggttagagca agcaatcgcc tacgtcaccg aactccacca aattgcccag
     4801 gatgcgcctc gcggctccgt gctggatgcc tgtaagaagc tggccaagag cgatggtcgc
     4861 gcggagtttg tgagagaatc ggatgcgaga tcccgaattc aaaatccgat attcctaaac
     4921 ctctgatgca ttgggattga catccaatcg gaatc
//
LOCUS       DATAQL010000076         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3824560, whole genome shotgun sequence.
ACCESSION   DATAQL010000076 DATAQL010000000
VERSION     DATAQL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3824560"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VHS97_01240"
     CDS             complement(<1..250)
                     /locus_tag="VHS97_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX3446843.1"
                     /translation="MSDERTQPPSKRRRQLARQQGQAVHSPELTAAAGWLVAVIVLGL
                     VGENLTRGFTELVSGSLTQPGALMAEPVAVVSRVREFVV"
     gene            complement(285..1055)
                     /locus_tag="VHS97_01245"
     CDS             complement(285..1055)
                     /locus_tag="VHS97_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX3446844.1"
                     /translation="MAVEIGDWNWPWVLSHAGVWAIVMARVLGLCLTAPALAVPELDW
                     RLRLLLAVVLGVVLIPVLDPLIVPPSDRSSAAWALVLEILTGGILGWWAALVIAGARM
                     AGELVAAQAGLSTAALFDPETGEEITVLGRLYGWVALAVFLALDGPLVLIGALVESYQ
                     AVPAGRLLISEGPAALAFAHVGQALEVALRAAAPPALALCLAGIVLGWLSRAASSLPF
                     TALALPIRSFLGVVLVLLSLGALVTTLSGAWDTFPWTP"
     gene            complement(1078..1338)
                     /locus_tag="VHS97_01250"
     CDS             complement(1078..1338)
                     /locus_tag="VHS97_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEX3446845.1"
                     /translation="MSHAIVWSREALRMSLLLGGPLLAAAFLVGLLVNIGQTLTQLHE
                     PVVGLVPRIAAVTVVLLLILPWLLTRWVSFAAELIESIPSLL"
     gene            complement(1398..2294)
                     /locus_tag="VHS97_01255"
     CDS             complement(1398..2294)
                     /locus_tag="VHS97_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX3446846.1"
                     /translation="MTPRHKGRLRWRVALSLAVTLFAAPGSPVLPSAISATDEPKAKA
                     VSEGISVNRPSAPNAAVAPSLSMPDLTSKDSMRTLQNVALFGLISLAPMALLMVTAFV
                     RINVVLILLRQAMGSPQVPGNQILTALALMLTALVMHPIGERVYQKAVLPYSSGRLPA
                     GEAWKAGTQPIKTFMVDQIVWTKHQDYLQTLYEYAVPPSPGHVEAAPGRPEDFPLRVV
                     APAYLLSELTTALLMGFYVYLPFLVIDLVVSAVLAATGLFMLPPALVATPIKLIVFVL
                     ADGWLLIASMLLSTFTVAPGGP"
     gene            complement(2291..2866)
                     /locus_tag="VHS97_01260"
     CDS             complement(2291..2866)
                     /locus_tag="VHS97_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX3446847.1"
                     /translation="MPRANRRRRALRAVLGLAVLLALGFAVDMLVLVRARVRADGSAA
                     TGGQAQAVPTAVAEAIGTPSSAQPHEEPRNAEDELRGSRLSPLRRLSKAARPGGSDTW
                     SLAMIGITLALAVCGGIIAAGRRFLPQGAGGGVKVIGRVSLSPKHSVYLLEVGRRRLL
                     VGAGPQGAPALISELDDFAENEANSPAGVEP"
BASE COUNT          722 a         1093 c          975 g          496 t
ORIGIN      
        1 caacgacaaa ttctcgtacg cgagacacga ccgcgaccgg ctcggccatg agcgctccag
       61 gctgggtcag cgatccgcta accaactcgg taaatccgcg tgtcaggttc tcgcccacca
      121 gcccgagcac gatcaccgcc acgagccagc cggcggccgc agtcaattcc gggctgtgca
      181 cagcctggcc ttgctgacgc gccagttgcc tgcgccgctt cgacggtggt tgcgtccgtt
      241 cgtcggacat gacatgacct caaggagggt tcgggcggcc agattcacgg cgtccaggga
      301 aacgtgtccc acgcgccgga aagcgtagtt accaacgcgc ccaggctgag taagaccagc
      361 acgacaccca ggaaactgcg gatcggcaac gccagcgctg tgaaggggag cgaggaagcc
      421 gcacggctca gccagcccag cacgattcca gccaagcaca aggccagcgc aggcggcgcg
      481 gcggcccgga gcgccacttc cagcgcttgg cccacgtgcg cgaacgccag cgccgcaggg
      541 ccttccgaaa tcaatagccg gccggcgggc acggcttgat aactctcgac cagggcaccg
      601 atcagaacga gcgggccatc gagcgcaaga aacacagcca gggcgaccca accgtaaagc
      661 cggccgagta cggtgatctc ctcacctgtc tcgggatcga acagcgcggc cgtcgatagc
      721 ccagcttgag cggccaccaa ttcgcccgcc atgcgcgccc cggctatcac aagcgcggcc
      781 caccagccga ggatgccgcc ggtcaagatc tccaacacca acgcccaggc ggcgctcgac
      841 cggtccgaag gaggaacgat caacgggtcc agcaccggga tcaacaccac accaagcacc
      901 acggcgagca agaggcgcag acgccagtcc aattcgggta ccgccagcgc cggggccgtg
      961 aggcataaac cgagcacacg tgccatcacg atcgcccaga cgccggcatg ggagagcacc
     1021 cagggccaat tccagtcgcc gatctccaca gccatgaacc aagacctatc ccttacttta
     1081 gagcaaacta gggattgact cgatcaattc agccgcgaac gacacccagc gggtgagcag
     1141 ccaaggtaga atcaaaagca ggaccacagt cacggctgcg atgcgcggaa ccagcccgac
     1201 cacgggctcg tgcaactgag tgagagtctg gccgatgttg accagtagac cgaccaggaa
     1261 ggccgccgcc agcagaggcc cccccaacaa cagcgacatc ctcaaggcct cgcgcgacca
     1321 gacgatcgca tggctcatgt ccacagccgg atctcccttc tcctggcgta acgggccgac
     1381 tgtctcgctg gttcgattca gggaccgccg ggtgcgaccg tgaaggtcga cagcagcatg
     1441 gaagcgatca gtaaccaacc gtcagccaag acaaacacga tcagcttgat cggggtcgcc
     1501 accagggccg gcggcagcat gaacaatccg gtcgccgcca gaactgcaga aaccaccagg
     1561 tcgatcacca gaaacggtaa gtacacgtaa aaacccatca acagcgctgt ggtcagctcg
     1621 ctgagcagat aggccggcgc cacgacgcgc agcggaaagt cctccggacg tcccggagcc
     1681 gcttcgacat ggcccgggct gggcggcacc gcatattcat aaagcgtctg gagatagtct
     1741 tgatgcttgg tccacacaat ctggtcgacc ataaacgtct tgatcggctg ggtgcccgcc
     1801 ttccaggcct cgccggccgg gagccgtccg gacgaatagg gcagaacggc cttttgatag
     1861 accctttcgc cgatcgggtg catcaccagg gccgtcagca tgagagccag cgcggtcagg
     1921 atctgattac ccggcacctg cgggctgccc atggcttgcc ggagcaagat cagaacaaca
     1981 ttgatacgca cgaatgccgt caccatcagc aaggccatcg gcgccaacga gatcaatcca
     2041 aagagcgcaa cattttgaag cgtccgcatc gagtctttgg acgtcaagtc tggcatcgac
     2101 aacgacggcg cgaccgccgc attgggggcc gagggccggt taacgcttat accttcggat
     2161 acggcttttg ctttgggctc gtcggtagcc gatatcgcgc ttggaagcac aggacttccc
     2221 ggggcggcga ataaagtcac cgccagagac agcgcgactc gccagcgcag tcggcctttg
     2281 tgtcgcggag tcatggctct actcctgcgg gagaattagc ttcgttctcg gcaaaatcat
     2341 cgagctcgct gatcaaggca ggcgctccct ggggccctgc cccaaccaga agcctgcgcc
     2401 ggccgacttc cagcagatag accgaatgct tgggagacag gctcacccgg ccgatcacct
     2461 tcacaccacc acccgcaccc tgaggcagga accggcggcc ggcggcgata atcccaccgc
     2521 atacggccag ggccagggtg atcccgatca tcgccagcga ccaggtgtcg gatccgccgg
     2581 gacgagccgc tttcgacagt cggcgcaacg gtgacagtcg cgaacctctg agttcgtctt
     2641 ccgcattccg aggctcttcg tgtggctgcg cactcgaggg ggtgccaatc gcttcggcca
     2701 ccgcggttgg aaccgcctgc gcctgacccc cggtcgcagc cgatccatcg gctctgacac
     2761 gggcccgcac cagaacgagc atgtccacgg cgaagccgag ggccaagagc acagccaacc
     2821 cgagaaccgc acgcagagct cggcgcctac gattcgctcg gggcatcggc agatcctctg
     2881 ctgaagaaga gcgggcatcg tgcccgcgtc ggagatttta ttgggccaaa acgcccgtga
     2941 tacttcctaa acccaattcc gttggattag cgttaactta tactcgcata gcaagttatg
     3001 atctcctccc ccctcgtggg ggagggccag ggtggggggc gtttctctcg gggaccgtgg
     3061 ccgagcgaat gctttggcac ttgcctcccc cctccctaac catcccccac aaggggggag
     3121 ggcgacagaa acgacctagg attagtaagt tggacgtaat tcaacggtat tggtcctaaa
     3181 ccaggacttc gtcgcgccca ctcggctcag gcagactgat acggccattg tcgtacaacc
     3241 ggcgcaaccg ctcggcgata tcgagctggg ccgcttcgga atccga
//
LOCUS       DATAQL010000077         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_770541, whole genome shotgun sequence.
ACCESSION   DATAQL010000077 DATAQL010000000
VERSION     DATAQL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770541"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VHS97_01265"
     CDS             <1..1660
                     /locus_tag="VHS97_01265"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX3446848.1"
                     /translation="PGSVVVNWGDGSAVQTLAAANLTLNGSPNGVVFTINASHTYAEE
                     GTHAYTVTVTDVGGATTVISGSAKIADAPLTATAVQPTVDTTEAALFPLPVFAPPAFS
                     GPVAEFSDGNPLPPTGSSSIADFTATIDWGDGTAPSAGTIVALLGTTTPTYAVTGSHT
                     YADAGVNGGAGTYAIHVFVQDDGGSRLTLTNTAHVADIPITVTGQLNPASDSGVSNSD
                     DITNVTQPDFFGKSEPLSHVTLSETLLSSGLSVPIGTVQAGIDGSWNIKSGISLADGS
                     YRISASAVDQFGETTTAAPVVITSDLVIDTVGPVITGAFFNRLNGQVDYTIQDPSPTS
                     GPPSGVWVNTMLDSSNYLLTKVHANRAYPGKWIVTNVTATPGAAPNSYDVAVTFNSGK
                     PIRGGFYLFTIRDSTNGNSSVQDITGNHLDGVFYGSFPSGNGINGSDFVAMLSAYHFK
                     IFAPQTIVGTASAANGGVGGPRVGAVHSGNFTRVVPVGGGPVFGTAPTQVSGTRTAHT
                     KKAVSRSKLATYAVAPKSHAVKTAVHDDALKALADQTKPTRLRK"
     gene            complement(1779..3875)
                     /gene="mutL"
                     /locus_tag="VHS97_01270"
     CDS             complement(1779..3875)
                     /gene="mutL"
                     /locus_tag="VHS97_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX3446849.1"
                     /translation="MGIIRQLTPAVINQIAAGEVVERPASVVKELLENAIDAQATRID
                     LSVERGGKDLVRIADNGIGMTPDDLIPAFQPHATSKLADADDLFRVRTLGFRGEALAA
                     IAEVAKVRCQTRQAQGAEGSELLIEGGIFGPVKKCGCPPGTVLEVRNLFYNIPVRRTF
                     LKSDMTESGHVADMFSRIALAHPEVHLTYRSGNKVIYDLPPVPGTRDRIATFFGRELA
                     ESLLWVEGQLDSMQLWGYVAHPSQSRSSAKGQFLFIGGRYVRDRSLSHALNEAYRGLL
                     MVGRMPVAFLHLEIPADEVDVNVHPTKVEVRFRDSQRVYSHLLSTLRQTFLKSDLHTR
                     LQATSEQAAAEPARVAVGAGAMAQPDMSYGSRQGSPYGSSVERLELDGAPADRQTVAS
                     WFESSRAQPRLPESIGAPAPPSWAQSLPLAFPVVAGEAFDEFSSSATAPNIAVPHSHQ
                     APVGPPSGAANGPSAGDRSGFDGPTQAGADYFRPIEAGEQADFHDGAKRGVSSLPPAA
                     HLKKAIQVHDSYLIAETGDGMMVIDQHALHERILYEELRTRVAEGRVESQRLLVPEPV
                     DLTAAEAAALVEHGELLAQLGLEVEPFGGDTVLVRSIPAMLPRVVPERLVRDLADHFH
                     SQPLPPTRDGLVAELLHMVACKAAVKAGQPLSALEIEALLERRHLVADSHHCPHGRPT
                     ALVFTKADLEKQFGRI"
     gene            complement(3854..>4125)
                     /locus_tag="VHS97_01275"
     CDS             complement(3854..>4125)
                     /locus_tag="VHS97_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3446850.1"
                     /translation="QSLEEIEKHYILETLKRCNGNREEAARQLGIGERTLYRKLKEYN
                     LGLDASAAGWSVESGRISIYERKIRLYNAWVVKPRRSTRWESSAS"
BASE COUNT          768 a         1375 c         1242 g          740 t
ORIGIN      
        1 gccgggctct gtcgtcgtca actggggcga cggctctgcc gtgcagacct tggcagccgc
       61 gaatctgacc ttgaatgggt cgcccaatgg cgtcgtcttc accatcaatg cgtcccatac
      121 ctacgccgag gaggggaccc acgcctatac ggtcactgtg accgatgttg gtggtgcgac
      181 gaccgtgatc tccggttcgg ccaaaatcgc cgacgcgccg cttaccgcga ccgccgtcca
      241 gccaacggtc gatacgaccg aggcagccct cttcccgctt ccggtgttcg cgccaccggc
      301 cttcagcggg ccggtcgccg agttcagcga cggcaatccc ctgcccccca cgggctcgtc
      361 ttcgatcgcc gacttcaccg ccacgatcga ctggggcgac ggcacagcgc cctcagccgg
      421 gacgatcgtc gccctcctgg gaaccaccac gccgacctac gcggtgactg gttcccacac
      481 ctacgcggac gcgggagtca acggcggcgc tgggacgtac gcgatccacg tcttcgtcca
      541 ggacgacggc ggctcgcgac taaccctcac caacaccgcc catgtcgccg acatcccgat
      601 cacggtcacc gggcaactca atccggcgag tgacagcgga gtgtcgaact ccgacgacat
      661 caccaacgtc acccagccgg acttcttcgg caagtccgaa ccgttgtccc atgtgacatt
      721 atccgaaaca ctgcttagca gtgggctctc cgtgccgatc ggcacggtgc aggcgggcat
      781 cgatggatcg tggaacatca agtccggcat ctcccttgcc gacggcagtt accggatctc
      841 ggcctcggcg gtcgaccagt ttggcgagac caccacggcc gccccggtcg tcatcacgtc
      901 cgacctggtg atcgacacgg tcggtccggt catcacaggg gccttcttca accggcttaa
      961 tggtcaggtt gactatacca tccaggaccc gagcccgact tcagggccac cctcgggagt
     1021 gtgggtcaac acgatgctcg actcgtccaa ctacctgctg accaaggttc acgccaacag
     1081 ggcgtatcct ggcaagtgga tcgtgacaaa cgtcaccgcg accccaggcg ccgccccgaa
     1141 ttcgtacgac gtcgccgtga ccttcaacag cggcaagcca atcagaggcg gattctacct
     1201 gttcacgatc cgcgactcca caaacggcaa ctcgtccgta caggacatca ccggtaatca
     1261 tctcgacggc gtattttacg gctcgttccc ttcggggaac ggcatcaacg gctccgattt
     1321 cgtcgcgatg ctctccgctt atcacttcaa gatcttcgcg ccccaaacga tcgtggggac
     1381 agccagcgcg gccaatggcg gcgtgggcgg tccacgcgtc ggagcggtgc acagcggcaa
     1441 ctttacacgg gtcgtcccgg tcggtggcgg cccggtgttt ggcaccgccc cgacgcaagt
     1501 cagcggaacg cgcaccgcgc acaccaagaa ggccgtttca aggtccaagc tggcgacata
     1561 cgccgtcgcc cccaagagtc atgcggtcaa gaccgctgtc cacgacgacg ccttgaaggc
     1621 actcgcggac cagaccaagc ccacacgcct gcgcaagtga gcggcgtcct tcgataaaaa
     1681 ctcattcgaa gatcggggct gggaccgtgc cggccccgat cgtgttcttt gatggcgttg
     1741 cgatagagcg cgtgcaaagc ggtctcgccg atcgtccttc agatcctgcc gaactgcttt
     1801 tcgagatcgg ccttggtgaa gaccagcgcg gtgggccggc cgtgcgggca gtgatgcgaa
     1861 tcggcgacca ggtgtcgtcg ctccaggagc gcctcgatct caagagccga caggggctgg
     1921 ccagccttca cagctgcctt gcaggcgacc atgtgcaaaa gctcggccac caatccgtct
     1981 cgggttggag gcaagggctg actatgaaaa tggtcggcaa ggtcacgtac caagcgctcg
     2041 gggaccacgc gcggcaacat tgctgggata cttcttacca gcacggtatc cccgccgaat
     2101 ggctcgacct ccaggccaag ctgcgccaag agctcgccgt gctcgaccag ggcggcggcc
     2161 tcggccgccg tcaggtccac cggctcgggg accaaaagcc gctgcgactc gactcgcccc
     2221 tccgccacac gcgtgcgtag ctcttcatac aagatccgct cgtggagcgc atgctggtca
     2281 atgaccatca tgccatcgcc ggtctcggcg atcagataac tgtcatggac ctggattgcc
     2341 ttctttaagt gtgcggccgg cggcaatgac gacactccgc gcttggcacc atcgtggaag
     2401 tccgcctgtt ccccggcttc gatcggtcga aaataatccg ctcccgcttg agtcgggccg
     2461 tcgaaccctg atcggtcgcc ggcggacggt ccgttcgccg cgcccgaagg cgggcccacg
     2521 ggcgcctgat ggctgtgagg cacagcaatg ttgggcgccg tggcggagga ggagaactcg
     2581 tcgaacgcct cacccgccac gactggaaac gccagcggca gcgattgagc ccaggaaggg
     2641 ggcgcgggcg cgccgattga ttcggggagg cgtggctggg cgcggctcga ctcaaaccat
     2701 gacgcgacgg tctggcggtc ggccggggcg ccatccagct cgagccgctc gacgctggaa
     2761 ccatagggcg agccctgacg ggagccgtaa ctcatatctg gctgcgccat cgccccggcg
     2821 ccgacggcga ctctggctgg ctccgcggca gcctgctcgg atgttgcctg cagccgcgtg
     2881 tgcaagtcac ttttcaagaa cgtctgccgc aacgtcgaaa gcaggtggct gtaaacgcgc
     2941 tgagagtcgc ggaaccgaac ttcgaccttg gtcggatgga cattgacatc gacctcgtcg
     3001 gcggggattt cgaggtggag aaacgccacc ggcatccggc cgaccatcag caacccgcga
     3061 taggcttcat tgagcgcatg ggagagcgag cggtcacgta cgtaccggcc cccgatgaac
     3121 aggaactgcc ccttggcact cgagcggctc tgggacggat gcgcgacata gccccagagt
     3181 tgcatggaat cgagctgccc ttccacccag agcaacgact cggcgagctc gcgcccgaaa
     3241 aacgtcgcga tccggtcgcg ggtacccggg accggcggca ggtcgtagat gaccttgttt
     3301 cccgagcggt aggtcaggtg aacttccggg tgcgccagcg cgatccgcga aaacatgtcg
     3361 gcgacgtgac cgctctcggt catatccgac ttgagaaacg tgcggcgcac cgggatgttg
     3421 tagaacaggt ttctgacttc caacacggtg ccggggggac agccgcattt cttgaccggg
     3481 ccgaaaatgc ccccttctat caagagctct gaaccttccg cgccttgggc ctggcgcgtc
     3541 tggcagcgga ccttggccac ctcggcaatc gccgccagtg cctccccgcg aaaacccagg
     3601 gtccgcacac ggaacaggtc gtcggcgtcg gccagtttac tcgtggcatg cggttgaaac
     3661 gccgggatca agtcatcagg ggtcatcccg atgccgttgt cggcaatccg caccagatct
     3721 ttaccacccc gttcaaccga taggtcgatc cgcgtagcct gggcgtcgat cgcgttttcg
     3781 agcagctctt tgaccacgct cgccggccgc tcgacaacct cgcccgcggc gatctgattg
     3841 atgaccgccg gagtcaactg gcggatgatt cccatcgtgt cgatctcctg ggtttgacga
     3901 cccaggcatt atacaatctg atcttccgtt cataaatgga gatccgacca gactcgacgc
     3961 tccaccctgc ggcgctcgca tctaacccca gattatattc ttttagtttt cgatagagcg
     4021 tccgctcgcc gataccgagc tgacgtgcgg cttcctcgcg gttgccgttg caacgtttca
     4081 gggtctcgag gatatagtgt ttttcgatct cctcgagcga ctggc
//
LOCUS       DATAQL010000078         6535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4893214, whole genome shotgun sequence.
ACCESSION   DATAQL010000078 DATAQL010000000
VERSION     DATAQL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4893214"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VHS97_01280"
     CDS             complement(<1..240)
                     /locus_tag="VHS97_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446851.1"
                     /translation="MTRRLRGWRVFPVCLAALLAVAGCGESEGEDQNDQSPPRTAGGM
                     RGQSGPARAGIKQIMTKVAKGATSLTSIIGNELNQD"
     gene            341..637
                     /locus_tag="VHS97_01285"
     CDS             341..637
                     /locus_tag="VHS97_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446852.1"
                     /translation="MSSPRLFAGDSNRNRRDLGSSLPYLTLSLPAPRPTIGDGAGNQA
                     GLFGQGRGLRVGLFFRLAVDMRLLRRGVFARWNVGQHSQQADEFHGLGEVAVER"
     gene            641..1312
                     /locus_tag="VHS97_01290"
     CDS             641..1312
                     /locus_tag="VHS97_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3446853.1"
                     /translation="MRRSVGSTAGDRDDYRANVAIDWGRINTYVFPASLSVGPLALAV
                     LTRGNYAAAWGNTNWGQGQSSESILPIPYLQSAFGHLGRITFASATDGSSNTVFMSEV
                     RQGTASDVRGLMWSVGAGGGSFNSRLTPNGTIDLLGQFNGFDQLGDSFSCVNNPGQDL
                     PCAALTNHGNNTAFSASRSRHAGGVNSLFGDGSVHFIKNSINPITWVALNSIQAAEVI
                     SSDSY"
     gene            1740..2723
                     /gene="msrP"
                     /locus_tag="VHS97_01295"
     CDS             1740..2723
                     /gene="msrP"
                     /locus_tag="VHS97_01295"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX3446854.1"
                     /translation="MRATDPIPASEITPLSVYLNRRSFINGGIAAASAVTTGWLYRRL
                     NRAGSAEVETPALTAVRAAPQGQEGIVSGVRVEEPMTPLLSISNYNNFYEFTTNKESV
                     ATVAAGFVSKPWQVAVEGMAHKPRVFDLDELIKTIPLEERVYRMRCVEGWSMVIPWSG
                     FPLAKLLERVQPMGDVEYVAFETLLDPARMPGQKTNVLRWPYVEGLRMDEATHPLTLL
                     AVGLYGHELPPQNGAPLRLVVPWKYGFKGIKSIVKITLMSKRPATTWNISAPTEYGFF
                     SNVNPEVDHPRWSQATEQRIGELGRRPTLMFNGYAAQVGHLYSGMDLRANF"
     gene            2738..4486
                     /locus_tag="VHS97_01300"
     CDS             2738..4486
                     /locus_tag="VHS97_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX3446855.1"
                     /translation="MKVTRFARFTVLLNCTVPVILLCWDAWRGQLGANPVNFAIRTTG
                     LLSLIFLVLTLIVTPASRITHWSWLGQFRRIFGLYAFFHGFLHFLIFFGFDRAASASS
                     TFSEIVTRPYLMVGTLGLVLMVPLAATSTDGMIRRLGGKRWKALHRLAYVVAMAGAIH
                     YYMLVKADVTQPVGFAIALAVLLGYRLVAHYLNLRSAYHKLLSASATTVASAATVPPG
                     KPKFWSGQLRVARVFDEAPGVRTFRLATPAGTRLPFDFLPGQYLNLSLMIDGKRVNRS
                     YTIASSPTRAGYCELTIKREERGIASRYVHDAVREGCLLDVSAPAGRFTFTGTEATSI
                     VMIAGGVGITPLMAKIRYLTDLSWTGDIHLLFAVRTVNDIIFREELEYLRRRFPNLHV
                     TVTLTRDDGTNWTGERGRISPEMLNRVVPQISAELVHICGPGEMTESTIRMLQELGVP
                     DERVRLESFSPAGRPDESASLPPEEYASTVVTVAGGEMALAGTPLGAASVTFARADKS
                     KQVSPRETILEAAEDLGVEIAYDCRAGICGQCKTKLLAGRVIMDVQDALNPLDRVNSM
                     ILSCQARCVDQVVVEA"
     gene            4523..5296
                     /locus_tag="VHS97_01305"
     CDS             4523..5296
                     /locus_tag="VHS97_01305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX3446856.1"
                     /translation="MNRHSVRRVVLFLSTLGASLFALASAETVRAQAPAGEASDAPVR
                     KELDEFRDKQSKTAPPDRLRAYEEGIEAVRKSGVTDKSLKVGDHAPDFELPNAAGKKV
                     KLSDLTARGLVIVTWYRGGWCPYCNIALRGFHKSLPEIRATGASLVAISPEMPNNSMS
                     TAEKNHLDFDVLSDRGNKVGHAYGLVYKIPNVITEQFKGRLDLAKYNGDDSAELPLGA
                     TYVIDREGIIRYAFVDADYRKRAEPSTVISVLRGLGKKR"
     gene            complement(5345..5749)
                     /locus_tag="VHS97_01310"
     CDS             complement(5345..5749)
                     /locus_tag="VHS97_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446857.1"
                     /translation="MPYLQLDTPFSYSTEAKQRLARRLGEIYSKHMTSNINRLTVAIR
                     ELGDGGVWRCGEGDPRPAALLMCDVRRGRSAEQRAELARKLIDACKEIVGLKDDNLNL
                     EFTQHAGDEMYHTLYGGLSDDWNAGEPDRLKS"
     gene            complement(5775..>6535)
                     /locus_tag="VHS97_01315"
     CDS             complement(5775..>6535)
                     /locus_tag="VHS97_01315"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX3446858.1"
                     /translation="LAPQLVTAGNIAAPFDAVLVTVKAFSLEQALDDFAPAVGPETMI
                     LPVLNGMKHVDILAARFGESSVVGCVCKVAATIDDEGQIVQLATFQDLIYGEFGGAQS
                     LRTDRLDAVMQGAGFDARLSPTIEREMWEKWILLATLGGITCLMRGTVGEIEAAPEGL
                     DFVHRFLDEVVAVVKAVGHPPSEDFMTSARALLTAKGSTQTSSMYRDLQKGSPIEADQ
                     IIGDLLARAQLAHVSTPLLATAYTHLSVYQSRIS"
BASE COUNT         1261 a         2004 c         1976 g         1294 t
ORIGIN      
        1 atcctggttc agctcgttgc cgataataga ggtcagcgag gtcgccccct tggctacctt
       61 agtcatgatt tgcttaatac ccgctcgcgc aggtccgctc tggccacgca ttccgcccgc
      121 agtgcgcggc ggtgactgat cgttttgatc ctcgccctcg ctctctccac aaccagcaac
      181 cgccagtaag gcggcaaggc agaccgggaa cacacgccat ccacgcaacc gtctcgtcat
      241 ctcaaaaacc ctcgtcgatt aaaataaatc gccaatacct gcctgaagag aggcccgtga
      301 accatttccg gttactttct tgcgtgcgcg gaaagtcttt ttgtcgagcc cgcgcctctt
      361 tgccggtgac tccaatcgga accgccgcga cctcggatca tcgctgccgt acctcacgct
      421 ctctctcccg gcgccgcgcc ctaccatcgg agacggagcc ggtaatcagg cgggcctatt
      481 cggtcagggg cgcggcttaa gggtcggact attcttccgg ttggccgtgg atatgcgact
      541 cctgcggcgc ggtgtgttcg cccgctggaa tgtcggccag cattcgcaac aagcggacga
      601 gttccacggt cttggtgagg tggccgtcga acggtgaggg ttgcggcgct cggtcggctc
      661 cacagcggga gatcgcgacg attaccgggc caacgtcgcg atcgactggg gaaggatcaa
      721 cacttacgtc tttccggcat cgctcagtgt aggtccattg gccctggcgg tcttaacgag
      781 aggaaactac gcggccgcct ggggaaacac gaactggggg caggggcagt cgtcggaatc
      841 gatcttgcca atcccctatt tgcagtcagc gtttgggcat ctcggccgga tcacgttcgc
      901 ctcagcgacc gacgggtcga gcaacactgt cttcatgtcc gaggtgcgcc aggggacggc
      961 cagtgacgtc aggggcctca tgtggtccgt aggcgcggga ggcggctcgt tcaattcccg
     1021 gttaactccc aacgggacca tcgacctcct cggccagttt aacggcttcg accaactcgg
     1081 tgattcgttc tcctgcgtca acaatccggg ccaggatctc ccgtgcgcgg ccttgaccaa
     1141 tcacggcaac aacacggcgt tctcggcctc gcggagtcgc cacgcgggcg gcgttaattc
     1201 tctctttggt gacggttcgg ttcacttcat caagaactcg attaatccga taacctgggt
     1261 cgcgctcaac tcgattcagg cggccgaagt gatcagctcc gacagctatt gaccaacgaa
     1321 gatagctctg caaatagtcg acattcggcg agagcgggag cggcttgagc gaggcgatgg
     1381 gttcgtctcc aaggacaatg ccggcacccc gctccccgct tgaatcggcc gcacatcgcc
     1441 cgctcttccc acgaccgttc gtagtcccca cgaaggttcg tggcgcgtct cgcccgactc
     1501 tcttccgcaa acgtcccacg caaaagatcc caattcgatg aagcggctgt cgttgcatcg
     1561 atttgaagaa cgtgtccggc ctaacggcac cgcggttgcg aagctgccaa caggagttct
     1621 cacggatctg gtatgttcgt gaacgtgccc attgaagaaa gcggctcatt ggcgaatctg
     1681 agaatcgccg gctggtgggt caccgcggac cgatcctgac tcgcgaatag ggccctgtta
     1741 tgagagcaac ggatccaatt cctgcaagtg aaatcacacc cctgtcggtg tacttgaacc
     1801 gccggtcgtt catcaacggg gggatcgcgg cggccagcgc ggtgacgacc ggatggctct
     1861 atcgccggct caatcgcgcg ggttcggccg aggtcgaaac gccggcgctc acggccgtga
     1921 gggccgcgcc gcaagggcaa gagggcatcg tcagcggggt tcgcgtcgag gagcccatga
     1981 cgccgttatt gagcatcagt aactacaaca acttttatga gttcacgacc aacaaggaaa
     2041 gcgtggccac cgtcgccgcc ggcttcgtgt cgaaaccatg gcaggtcgca gtcgaaggga
     2101 tggcgcacaa gccgagggta ttcgatctcg acgagttgat aaagaccatc ccgctggaag
     2161 agcgagtgta ccggatgcgt tgcgtcgagg gatggtcgat ggtgatcccg tggtcgggat
     2221 tcccgctggc caaattgctc gagcgggtcc aaccgatggg agatgtcgaa tacgttgcgt
     2281 tcgaaaccct gctcgacccc gcgcgcatgc cgggccaaaa gacgaacgtc ctccgctggc
     2341 cttacgtcga gggtcttcgg atggacgaag cgacgcaccc gctgacgctc ttggcggtcg
     2401 gactctacgg gcacgagttg cccccgcaaa acggcgcgcc gcttcggctg gtggtgccct
     2461 ggaaatacgg cttcaaggga atcaagtcga tcgtgaagat cacgctcatg tcaaagcggc
     2521 ccgcgacgac ctggaacata agcgcaccga ctgaatacgg gttcttctcc aacgttaacc
     2581 ctgaggtcga tcacccgcgg tggagtcagg cgaccgagca acggatcggc gaattgggtc
     2641 gtcggccgac gcttatgttc aacggctatg ctgcgcaggt ggggcatctc tattccggca
     2701 tggacctgcg cgccaatttc tgaagcgggc ggcaaggatg aaggtcacac gattcgctag
     2761 attcaccgta ctactcaatt gcaccgtgcc ggtgatcctc ctctgctggg acgcgtggag
     2821 gggccagctc ggcgccaacc cggtcaactt cgccatccgg accaccgggc tgctctcgct
     2881 gatcttcctg gtcctcacac tcatcgtcac tcccgcaagc cgcatcaccc attggagctg
     2941 gctgggccag tttcgccgga tatttggact gtacgcgttc tttcatgggt tcctgcattt
     3001 cttgattttc ttcgggttcg accgcgcggc gagcgcgagc agtacttttt cagagatcgt
     3061 cacgcgtccc tatctgatgg tgggaacgct cggtctggtg ctcatggtgc cgctggcggc
     3121 gacctcgacc gacggcatga tcaggcgcct gggaggcaag cgatggaagg cgctgcacag
     3181 gctggcttac gtcgtcgcga tggccggtgc tatccactac tacatgctcg tgaaggccga
     3241 cgtaactcag ccggtagggt tcgccatcgc actcgcggtt ctgctcgggt accgactggt
     3301 cgcccactac ctgaatctcc ggtccgccta tcacaagctt ctttcggcct ctgccacaac
     3361 agttgcatct gccgccaccg tcccgccggg caagcccaag ttctggtcgg gtcaactgag
     3421 ggtcgcacga gtcttcgacg aggcgccggg agttcgcacg ttccggctcg caacgcccgc
     3481 cggtacccgc ctgcccttcg acttcttgcc gggccagtat ctgaacctct cactcatgat
     3541 cgacggcaaa agggtcaacc gatcctacac aatcgcctcg tcacccaccc gcgccggcta
     3601 ttgcgaattg acgatcaagc gggaggagcg tgggatcgcg tcgcggtacg tccacgacgc
     3661 tgtccgcgag gggtgcctgc tcgacgtctc cgcgccggcg ggccgcttca cattcacggg
     3721 gaccgaggcc acgagcatcg tgatgattgc gggcggagtc ggcatcacgc ccttgatggc
     3781 caagatccgc tatctcaccg acctcagttg gaccggggac atccatcttc tattcgccgt
     3841 gaggactgtc aacgacatca tctttcgcga agaactcgaa tatctgcgac ggcggtttcc
     3901 caacctgcac gtcaccgtga ccctcacccg cgacgacggg acgaactgga caggtgagcg
     3961 cggcaggatc tcccccgaaa tgttgaatcg ggtcgtcccc cagatttcgg cggaactcgt
     4021 ccatatttgc gggcccggtg aaatgacgga atcgaccatc cggatgctgc aagagctggg
     4081 cgtgcccgac gagcgggtca ggctcgagtc attcagtccg gcgggccggc ccgatgagtc
     4141 cgcatcattg ccaccggaag agtacgcgag cactgtcgtg acggtggccg gcggggagat
     4201 ggcgttagcc gggactcccc tcggggcggc ctcggttacg ttcgcgcgag cagataaatc
     4261 gaagcaagtt tccccgcgcg agaccatact cgaagcggcg gaagacctgg gcgtcgaaat
     4321 cgcgtacgac tgccgggccg gcatctgcgg ccagtgcaag accaagctgc tcgctggtcg
     4381 cgtcatcatg gacgtccagg atgctctcaa tcctctcgat cgtgtgaaca gcatgattct
     4441 cagttgtcag gcccgctgcg tcgatcaagt cgttgtggaa gcttgagtca cggcgtgtaa
     4501 tttcagagga atacaaatgt tcatgaatcg tcattctgtg cgtcgagtcg tgttgtttct
     4561 gtcgacccta ggtgcgtccc tcttcgcgct cgcgagcgcc gagaccgtac gcgcccaagc
     4621 gccagcgggt gaagcgagtg acgcccccgt ccgtaaagag ctcgacgaat ttcgcgacaa
     4681 gcagtcgaaa accgccccgc cggaccgcct ccgagcctac gaggaaggga tcgaggcggt
     4741 gcgcaagtcc ggtgtaaccg acaagtccct gaaagttggc gaccacgcgc cggacttcga
     4801 gctcccaaat gcggccggca aaaaggtgaa gctgtcggac ctgacggcgc gaggtttggt
     4861 cattgtgacg tggtatcgcg ggggctggtg tccgtactgc aatatcgcct tgcgcggctt
     4921 ccacaagtca ttgccggaga ttcgtgcgac cggcgcgagc ttggtcgcca tctcgccgga
     4981 gatgcccaac aacagcatga gtacagcgga gaaaaaccac ctcgactttg acgtgctgag
     5041 tgaccgtggt aacaaagtag gccatgccta tgggctggtg tacaagatcc ccaatgtcat
     5101 caccgagcaa ttcaagggca ggctcgacct ggccaagtac aacggcgacg attccgccga
     5161 actccctctc ggtgccacct acgtcatcga ccgcgagggg atcatccgct acgcgttcgt
     5221 agacgcggat taccggaagc gagccgagcc ctccaccgtg atcagcgtac ttcgcgggct
     5281 gggcaagaag cggtgaactt cgtcccgtcg ggtgtatctg ggcgccgaga agctgatttg
     5341 attgtcagga tttcagccgg tctggttcgc cggcattcca atcatcgctc agaccgccgt
     5401 agagcgtatg gtacatctcg tcaccggcgt gctgggtgaa ctcgaggttg agattatcat
     5461 ccttgagccc gacaatttcc ttacacgcat cgatcagttt tcgcgcgagc tcggcccgct
     5521 gctcggccga gcggccgcgg cggacgtcgc acatcaagag cgccgccggc cgcgggtctc
     5581 cttctccaca gcgccagacg cccccgtcgc caagctctcg gatggcgaca gtaagtcgat
     5641 taatattgga agtcatatgt tttgaataaa tctcgccgag ccgccgggcg aggcgttgtt
     5701 tggcttccgt cgaataggaa aacggcgtgt caagttggag gtaaggcatc gcggaaatcc
     5761 tccgggtcaa ttggttagga tatcctgctc tggtagaccg acagatgggt ataggctgtc
     5821 gccaggagag gtgtcgatac gtgagcgagt tgtgcgcgtg ccaagagatc gccgatgatc
     5881 tggtcggcct caatgggact tcctttctgt aggtcccggt acatcgacga agtctgggtc
     5941 gagcccttcg ccgtcaacag tgcccttgcg ctcgtcataa agtcttcgct cggcgggtgt
     6001 cccactgctt tgaccaccgc gacgacctcg tccaggaacc gatgaacgaa gtcaagaccc
     6061 tccggtgccg cctcgatttc gccgacagtg ccacgcatca ggcacgtgat cccgcccagg
     6121 gtggccagga ggatccattt ctcccacatc tcgcgctcga tcgtcggcga gagtcgggca
     6181 tcgaagcctg ccccttgcat gaccgcgtcg agccgatcgg tgcgaaggga ttgagcgcca
     6241 ccgaattcgc cgtatatgag gtcctggaag gtggcgagct gcacgatctg cccctcgtcg
     6301 tcaatggtgg ccgcgacctt gcagacgcat cccacaacag agctctcgcc gaagcgcgcg
     6361 gcgagtatat cgacatgctt catgccattc agcacgggta ggatcatcgt ctcgggaccc
     6421 acggcgggag cgaaatcgtc gagggcctgt tcgagtgaaa acgccttcac ggtcaccaaa
     6481 accgcatcga agggcgcagc gatattgccg gcggtaacga gttgcggcgc cagcg
//
LOCUS       DATAQL010000079         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3977233, whole genome shotgun sequence.
ACCESSION   DATAQL010000079 DATAQL010000000
VERSION     DATAQL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3977233"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..505)
                     /locus_tag="VHS97_01320"
     CDS             complement(152..505)
                     /locus_tag="VHS97_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3446859.1"
                     /translation="MKTAFKTHEGRVVLVTGAAQGIGQAIALAFAERGARVIATDLKS
                     PRDTVSKIGPSASAFELDVTQEEQWSSLAAQVRDIGDVDIVVNNAGYFPNRPSTNWIY
                     RRGERRLQRIWTRTS"
     gene            complement(502..1086)
                     /locus_tag="VHS97_01325"
     CDS             complement(502..1086)
                     /locus_tag="VHS97_01325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3446860.1"
                     /translation="MMTISVIVGSTRQGRFSEKPAQWILQQLQKREGIEGRLLDLRDF
                     PMPFFDQPMPPAMPGRPPYEHEVVKKWTARIAASDGFVFVTPEYNYGPSAVLKNAIDW
                     VYPEWNRKAAAFVSYGGAMGVRAVQQLRETAVEVQLAPVRSAVHIPPATLWAHFQGGD
                     VEKGLAELTRQANAMIDDLLWWTTALKTARGAAK"
     gene            complement(1133..2236)
                     /locus_tag="VHS97_01330"
     CDS             complement(1133..2236)
                     /locus_tag="VHS97_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX3446861.1"
                     /translation="MKAVRLLEYGGQLVLDDVPTPAIKRDEVLVKIKSTAVNHLDLVE
                     ASGTARQIFPLDLPWIPGHEFSGVVEQIGSDVAAYAPGDAVFGNTTGMGAYAEYLTVK
                     ASAIAKKPSNLSFEEAASVPVASQTAWEGIFTNGHLEKGQTILIHGGAGAVGAYAVQF
                     ASHTGATVIATAGGDDKAYLKSIGASRVIDYREEQFEKVLREKVDVVFDLVGGDTQKR
                     SFLVLKEGGHLVSAVQPVSQEDAARYRVSALMMRLAPSGEMLARIGRLLEEGTIRPDV
                     ATVYALEDAAQAWKNIARNLPGVHGISPSAPGVSRDKSHGKIVLRVALSDHEIKGRAY
                     ELFLARGSQPGRELEDWLQAERELTAERPRRAD"
     gene            complement(2284..>2638)
                     /locus_tag="VHS97_01335"
     CDS             complement(2284..>2638)
                     /locus_tag="VHS97_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446862.1"
                     /translation="VWALGPETKLQMIAVEDIGWFGARAFNDAALNRREIDLAGDVRR
                     LPEAAEILTKAMGRAIAFAQTPIEQVRQYSKEMATMLEWFERVGYSADIVGLEREFGR
                     KLTKLPDWARRQERF"
BASE COUNT          495 a          837 c          727 g          579 t
ORIGIN      
        1 tttgccagac ctctcatgaa accgatgatt cccatctttg aagtgatgta gtgactcatt
       61 cctgttatcg ccaggccgac catgttcgaa gaaattccga cgaagcggcc ccactttttc
      121 ctgcgcatgc cgggcaagaa atatttcgcg ctcaggaagt gcgagtccag attcgttgca
      181 atcgtctttc gccacgtcga taaatccaat tcgtcgatgg acgattcgga aaatagcccg
      241 cgttgttcac gactatgtcc acgtcgccga tatctcgaac ctgggcggcg agcgaactcc
      301 actgctcttc ttgtgtcaca tcgagttcga aggcggacgc cgacggaccg atcttactga
      361 cggtgtcgcg cggcgacttg aggtcggtcg caatgacccg tgcgccgcgt tctgcgaacg
      421 ccaacgcgat cgcttgaccg atcccttgag cggcgccggt gaccaagaca acgcgtccct
      481 cgtgcgtttt gaatgcagtt ttcattttgc ggccccgcgt gccgtcttga gggccgtcgt
      541 ccaccagaga agatcgtcga tcatggcgtt cgcttgcctc gtcaactcgg ccaggccttt
      601 ttccacatcg ccgccttgaa aatgcgccca tagcgtcgcc gggggaatgt gaacggccga
      661 tcgaaccggc gccaattgga cttcgacggc tgtctcgcga agttgctgaa cggcgcgcac
      721 gcccatcgcg ccgccatagc tgacaaacgc cgctgccttg cggttccact ccgggtagac
      781 ccaatcgata gcgttcttca gcactgcgga gggaccgtag ttgtattccg gcgtgacgaa
      841 tacaaaacca tccgacgctg ctattcgggc agtccatttc ttcaccacct catgttcgta
      901 gggcgggcgg cccggcatag ccgggggcat gggctgatcg aagaaaggca tcgggaagtc
      961 tcggagatcg aggagccgac cttcaatccc ttcccgcttt tgcagctgct gaagaatcca
     1021 ctgcgccggc ttttcggaaa accggccctg gcgtgtactg ccgacgatca ctgaaatggt
     1081 catcatctta tttgctcctt gaggattgtt tccatcgacg ctgcgctcag actcaatccg
     1141 ctctgcgcgg acgctcagcc gtgagctccc gttcggcctg gagccaatct tccagctcac
     1201 ggccaggttg tgacccgcgc gcgagaaaaa gttcgtaggc gcggcccttg atttcgtggt
     1261 cactcaaggc cacacgaagc acgatcttgc cgtgagactt gtctcttgac actcctggag
     1321 cgctgggcga aatcccatga acccctggca ggttccgggc gatgttcttc caggcttggg
     1381 ccgcatcctc gagcgcgtac acagtcgcga cgtctggccg tatcgtcccc tcttcgagca
     1441 gtcgcccgat tctcgcgagc atctctcctg agggcgcgag cctcatcatc agcgccgaca
     1501 cacggtacct ggcggcgtct tcctgcgaga cgggctggac ggcagagacg agatggccgc
     1561 cttccttcaa gaccaggaac gaccgcttct gtgtatcgcc gccgaccaaa tcgaaaacca
     1621 catcgacctt ctctcgcaaa accttctcaa actgctcctc ccgatagtcg ataacccggc
     1681 tggctccgat ggacttgagg tacgccttat cgtcaccgcc tgcagtggca atgacggtgg
     1741 cgcccgtatg cgatgcgaat tgcacggcat aggcgcctac tgcgccggca ccgccatgaa
     1801 tgaggatcgt ttgtcctttc tcgaggtgac cgttggtgaa gattccctcc catgccgttt
     1861 gggaggccac cggcactgat gccgcttcct caaaggagag gttggatggt ttctttgcga
     1921 tggccgacgc cttgacagtc aggtattccg cgtacgcacc cattccagtg gtgttgccga
     1981 acacggcatc acctggcgca tatgccgcaa cgtcactacc aatttgttcg acgactcctg
     2041 aaaactcatg accaggtatc cagggcaggt cgagcgggaa tatctgtctt gcggttcctg
     2101 aggcctccac aagatccaga tgattgacgg cggtactttt gatcttgact aggacctcgt
     2161 cgcgtttaat tgccggcgtc ggtacatcgt ccaacaccaa ctgcccaccg tattccaaca
     2221 atctgacggc tttcatgcga gttctcctta aagtctgccc aattggcgcc acgcgcgggc
     2281 gcctcagaat cgctcctggc ggcgtgccca gtcggggagc ttcgtgagct tgcggccgaa
     2341 ctcgcgctcc aggccaacga tatcggcgct gtagccaacg cgctcgaacc attcgagcat
     2401 cgtcgccatt tccttgctgt actgccggac ctgttcgatg ggcgtctgcg cgaatgcaat
     2461 cgcccggccc atcgccttcg tcaggatctc cgccgcctcc ggcagccttc gtacgtcgcc
     2521 ggcgaggtcg atttcgcggc ggttcaaggc agcgtcgttg aacgcgcgcg cgccgaacca
     2581 gccgatgtcc tccacggcaa tcatttgtag cttcgtctcc ggcccgagcg cccagacc
//
LOCUS       DATAQL010000080         3808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3519667, whole genome shotgun sequence.
ACCESSION   DATAQL010000080 DATAQL010000000
VERSION     DATAQL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519667"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..413
                     /locus_tag="VHS97_01340"
     CDS             156..413
                     /locus_tag="VHS97_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446863.1"
                     /translation="MAALLFCLPPLTWTATGLLAGWLAARRGKSWRWGFALGFFCSTA
                     GLALAALPRKSSLAQARQGDSSGPGQGEVAVPVGSAVGQSR"
     gene            complement(1113..1187)
                     /locus_tag="VHS97_01345"
     tRNA            complement(1113..1187)
                     /locus_tag="VHS97_01345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1150..1152),aa:Pro,seq:ggg)
     gene            1299..2252
                     /gene="pyrF"
                     /locus_tag="VHS97_01350"
     CDS             1299..2252
                     /gene="pyrF"
                     /locus_tag="VHS97_01350"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX3446864.1"
                     /translation="MHFADRLFKAIRRVGNPVVVGIDPRPEELPTGFLERFPGDSSGV
                     AQALLAFGRDVLDVVSPLVAVVKFQSAFYEAYGPTGFAALHATAAIAHEKGLIVIIDG
                     KRNDIGSTAEAYARGYLGKVPVAGSFEPSWQADAITVNPYLGSDGVTPFVKVAAREHK
                     GVFVLVRTSNSSAGDFQDLLFDGKPLYRHVGDRLARWAQGHVGEEGYSLVGAVVGATY
                     PEQLAELRQSLPGIVLLVPGYGTQGGSAQDVSAAFDENGMGALINNSRGITFAYERPA
                     YRARFGGDWQSAIAQAVRDMIDDLATNSRAGRLGTAATLAK"
     gene            complement(2307..2552)
                     /locus_tag="VHS97_01355"
     CDS             complement(2307..2552)
                     /locus_tag="VHS97_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446865.1"
                     /translation="MARAWLKSAGAAALILAATAASATACPNCKEAVNLDAGEVANMS
                     SGYNWSVVFMLAVPFSLLSTGAFLVRRAVKSGVLPEM"
     gene            complement(2689..>3808)
                     /locus_tag="VHS97_01360"
     CDS             complement(2689..>3808)
                     /locus_tag="VHS97_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX3446866.1"
                     /translation="NVIVTPELPGTITQTASVTSSERQLIPADAAATATVLESPGSLQ
                     FSAASYAVPETAGSAYLTVTRTGGSRGPVTVNYQTVPVNATPGLDFNSTSGTLTFAAG
                     QTSAVIQVPVLADPWDDQNERVNVVLSSPGGGATLGAIASAQLTIIDVDPKRTPPHVS
                     QLSWTGSAQWITSVVLTFDSPLNTAYATNAANYLMTVQSAGNPVITFSMINYSIANRT
                     VTLVPSAPLPAGHFYRLQVMGTGATAIRDLAGNLLAGTTNGAAGTNYVTSFGQGTKLQ
                     YVDNSGNQVTVKLTGPGYLEDVLDSTGQGVVLTVFGEVPHRTSLSGSVRKVRGKSGRT
                     NIGAIKGLGKFGDVRVSLRSPTFLVNQYPFQQKGRGAL"
BASE COUNT          743 a         1138 c         1163 g          764 t
ORIGIN      
        1 cgtcgttgaa cttcgggagc gttgctcgac aaaatccgcc cgtcgcttac actaacaccg
       61 acttgctcga gccgttggcg aagtaggcgg cagcgtcacg tctggaacag taggaccagc
      121 gctcgtcaga gaggaacctc aggagagaga ccgctgtggc cgcactgcta ttctgtttgc
      181 ccccgctcac ctggaccgcc accggccttc tggcaggttg gttggccgcc cgccgcggca
      241 agtcgtggcg ctggggcttt gcactcggct tcttctgcag tacggccggg ctggcattag
      301 cagccctgcc gcgcaaaagt agcctcgcgc aggctcggca gggggatagc tctggccccg
      361 gccaaggcga agtggcagtg ccggtcgggt cggctgtcgg gcagtcacgc tgagcaaagc
      421 gcgcaggcga gctgctgcag cccgtcagtc aaggcggtcc cagacatcgc ggcggcggcg
      481 tcgccatcac caggcaatgc ccgtgcgggt cacttctcgg gccacggttc gttgtccgta
      541 agcacctcgt tgagcactcc cctataagaa atcagtcgtt gtatgattgc aacgacgtaa
      601 ctggggtcga agatgcggtt ttggcggcca ttgatcacat tttgcttatc tatttagcca
      661 gcagcagttt gatggccagt ttgatgattg cgggtctaac tcgtggtcac gccgtttttt
      721 gtgtagtagc tttcaacatg ggggcggtat ttggccacca gctagcgaca aagatgtcga
      781 tgtggagaag tgcaccgcgt ttcatcttga ccctggttct gtcgataagc agcgtgcgga
      841 cgatctcgtg aaattcgcct tgcgttcgga agtgtcgtgg atactacggt aaatattgcg
      901 gccgttgaca tgaacaacag cctgacctaa gccacggtgg agaacgatat ggcggaacgg
      961 gggacttgcg cgacatcgtt gtactctcca ggatggccgc agcaaccacg gtactactgt
     1021 ggtacgactg cctgctccgg agcacatacg ccgcaccgcc gaacatggcg ggtaagctac
     1081 aagtaaaacc tttcggcaac gctcgtaacc agtcgggacg gccggatttg aaccggcgac
     1141 ctcttgcacc ccaagcaagc gcgctagcca agctgcgcta cgccccgaac ccttgtcagg
     1201 ataacccgca ctaccagtgc cgtctatccc agccaagctc tttgattgta gaatccacca
     1261 tcgatctgtc aatccaaccg caacgggagg cacgtctcgt gcatttcgcg gaccgactat
     1321 ttaaggcgat tcgtcgcgtc ggcaacccgg tggtcgtcgg aatcgacccc aggcccgaag
     1381 aactgccgac cggattcttg gaacgatttc caggggactc gagcggagtc gcccaggcgc
     1441 tgctcgcttt cggccgcgac gtgctcgacg ttgtgtctcc gttggtcgcc gtggtcaagt
     1501 ttcagtccgc cttttacgag gcctatggtc ccactggttt tgctgcgctc cacgcgaccg
     1561 cggcgatcgc tcatgaaaaa gggctgatcg tcatcatcga tggcaagcgc aatgacatcg
     1621 gctcgaccgc cgaggcctac gcacgcggct atctgggaaa ggtccccgtc gcgggctcgt
     1681 tcgagccctc gtggcaggcc gacgcgatca ccgtcaatcc ctatctggga agcgacggcg
     1741 taacgccgtt cgtcaaagtc gcagccagag aacataaggg ggtttttgtg ctcgtgcgca
     1801 cgagcaattc ctccgcgggc gattttcagg atttactctt cgatggtaag ccactctata
     1861 gacacgtcgg cgatcgtctc gcgcgctggg ctcaagggca cgtcggcgaa gagggttaca
     1921 gcctcgtggg tgcagtagtg ggcgcgacct atcccgagca actggccgaa ttgcgacaaa
     1981 gcctgcccgg gatcgttttg ctcgtccccg gctacggcac tcaaggaggc tcggcccaag
     2041 acgtttcggc ggctttcgac gaaaacggaa tgggcgcact catcaataat tcccgaggaa
     2101 ttacgttcgc ctatgagcgc ccggcctacc gggcccggtt cggcggcgat tggcaatctg
     2161 cgatcgctca ggccgtccgc gatatgatcg acgacctcgc gacgaattca cgtgcgggac
     2221 gcctgggaac agcggccacg cttgcaaagt gaaatctcat gctccggccg cagcgacgac
     2281 actacggtct gaatcgcgac gggtggtcac atctcgggca gtacgccact cttgaccgcg
     2341 cgacgaacaa ggaaggcacc cgtgctcagt agcgaaaacg gcaccgccaa catgaagacc
     2401 acgctccagt tgtagccgct cgacatgttg gcgacctcgc ctgcatccaa gttgacagcc
     2461 tccttgcaat tcggacaagc cgtggcgctc gcggccgtcg ccgccagaat caaggcggcc
     2521 gcgcctgcgc ttttcaacca agctcgtgcc atcggaagat gccatcccat cagagccgcc
     2581 ccagagtcgc ttggggactg tatcgtttcc cctcgatggc aatattctag accccggcgg
     2641 cgggcgtcgt caaccaaagt cgagcgcgcc cgccagtttg gactcgtttc aaagggcccc
     2701 gcggcccttc tgctggaacg gatactgatt gacgagaaaa gtcggcgacc tgagcgagac
     2761 tcgcacgtcg ccgaatttgc cgagcccctt aatcgcgccg atattcgttc gaccgctctt
     2821 gccgcgcacc ttacggacac ttcccgacag gctcgtacga tgcggcactt cgccaaacac
     2881 ggtgagaacg actccctgtc cggtcgaatc gaggacatct tcaagatagc ccggcccggt
     2941 caacttcacg gtgacctgat tgccggaatt gtccacgtat tgcagcttgg tcccttgtcc
     3001 gaaggacgtg acataattcg tcccggccgc gccgttcgtc gtgccggcaa gaagattgcc
     3061 ggccagatcg cgaattgctg tcgcacccgt gcccatgacc tgaagtctgt aaaagtggcc
     3121 ggccggaagc ggagccgagg gcacgagggt gaccgtgcgg ttggcgatac tgtaattgat
     3181 catgctgaat gtgatgaccg gattacctgc cgattggacc gtcatgaggt aattggctgc
     3241 gttcgtggcg taagccgtat ttagcggcga atcgaaggtc aggaccacgc tggtgatcca
     3301 ttgggccgaa ccggtccagc tcaattgcga aacatgggga ggagtccgct tggggtcgac
     3361 gtcgatgatg gtcagttgag ccgaagcaat cgcgcccaag gtggctccgc cgccggggga
     3421 gctaagcacg acgttaaccc gctcgttttg atcgtcccag gggtcggcca ggaccggtac
     3481 ctggatcaca gccgaagtct ggccggcggc gaacgtgagc gtgcccgaag tcgaattgaa
     3541 atcgagtccc ggagttgcgt tgaccgggac agtttgataa ttgaccgtga ccgggccgcg
     3601 cgagccgccg gtgcgagtca cagtcaggta ggcggacccg gcggtttcgg gcacggcata
     3661 gcttgctgca ctgaattgca aactgcccgg cgattcgaga accgtcgccg tcgccgcggc
     3721 gtcggcgggt atgagttgtc gctcacttga ggtgacgctt gcggtctgcg tgattgttcc
     3781 cggcaactct ggagtgacga ttacgttg
//
LOCUS       DATAQL010000081         7590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_160404, whole genome shotgun sequence.
ACCESSION   DATAQL010000081 DATAQL010000000
VERSION     DATAQL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160404"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VHS97_01365"
     CDS             <1..1018
                     /locus_tag="VHS97_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX3446867.1"
                     /translation="TSRGASLNGTSVFAAMLRSERHELRTAIRLSDELAEWADGIVRF
                     APYPGPPDREEAEWYEQWLADDADRWLIYVVRDFDAVAEYWKTVADRLPAANEPERRA
                     EAEERRSKGANWVSRLPNKAKKAADPYAWFEVDTGWNPPRTCTNLTGEWAGGIDGVAA
                     GLTVHEAIRSRSCRLLLEADGKPFVMEKTVGAGRVLVVANGSFLLNEGVANPARRPLA
                     ERVLDWVNGRTARVAMVDGPSVLGEGEGMPSLWDLLWRIPPLRWVAIQVGLAAFLAAL
                     ARAPRLGRPRPDPPSGADRPAEHAVALGALLAKTGAAREANELVDSYRRWRTPHPRKP
                     GLHP"
     gene            1031..2080
                     /locus_tag="VHS97_01370"
     CDS             1031..2080
                     /locus_tag="VHS97_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3446868.1"
                     /translation="MDDNELASGASSQPGQQQVGEDAAGLSDGRRGPNELVSWSRRVL
                     GEVNKVFVGQPTLVRGVLASLLAEGHVLIESVPGLGKTLLVRVLGQVLGCSFNRIQFT
                     PDLMPSDVTGSPIYDERINDFRFRPGPVFTQILLADEINRAPAKTHSALLEIMQENRV
                     TIDGVSYPIEPPFLVMATQNPIESEGTYNLPEAQLDRFLFKLVVAYPSASEETDILKL
                     HTRGFGIDRGIADRLSVVTSPAEVRAMQRRAESVLVDDHVLGYIATLVRKTRQWPTFS
                     LGASPRAGVAILRGARAVAAMEGREYILPDDVQDVLLPALRHRVLLTPEAEVEGQSAD
                     ELLTELIRSVEVPLR"
     gene            2077..3399
                     /locus_tag="VHS97_01375"
     CDS             2077..3399
                     /locus_tag="VHS97_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3446869.1"
                     /translation="MKMVWPGRALGVALLVPALLSLVLFIREEVLPLVLAIDGIVSFV
                     ALIDLASLVGAGRLRVERQVGRVCSLNEHQDVVLSIENVGRRARSLRLRDDVPDEFSA
                     DPAAFDVTVPSRRQASLTYRFVPKRRGTYVFQQVDALVDSRLGFWRGQYSWPLRTEVR
                     VYPNIHQVARFTMLARRDRLSTMGLRRSRRLGTDNEFERLRDYIEGDDPRHLDWRATA
                     RRRKLTCRAFQANQSQRIMFLIDCGRLMAGDTGDGLSPLDHAFNAMLLLAHVALIRGD
                     QVGMLAFSDRVCAYVAPGGGPRRINRLVHGVHNIFPEMVEPRYDRAFIELEQRCRKRS
                     LVVLMTNLFDELSAELAGEYLRNLSGRHLPLGVFLRDHDLFAMADRAAAGGAGLYQGA
                     AAASLLNWRERVLAGLRMSGVLTLDVFPEELTAVLVNRYIEIKARHLL"
     gene            3441..4322
                     /locus_tag="VHS97_01380"
     CDS             3441..4322
                     /locus_tag="VHS97_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446870.1"
                     /translation="MDSFQPPSDHDRENPYAPPQSALLPRARDWSAAGVPFTVGDVFE
                     RSWSIFKTRFWSCLSVVWGVVGINWAISFGMNVLLAGLAEIARDETVYRLLYVLTLFG
                     AIVLQVWVAWVGQTIALLKIVRGEPVAFEDVFKGGRMVLTTILASIVMLAVIAVPILI
                     AVGAITAGLVLMQNQSGLGAVLLFLVVSVPIGVVVVFLTVRLYMYYYMVIDRDAGVFE
                     SFRQSWQCTKYQASTITLVFCLELAIVLAGFLALCVGLIFAIPLANLLVPVTYQFLTG
                     TGKSGGTEPEFYWENEN"
     gene            complement(4334..4900)
                     /locus_tag="VHS97_01385"
     CDS             complement(4334..4900)
                     /locus_tag="VHS97_01385"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3446871.1"
                     /translation="MTEAHSPPNRTLMYLAIALALFWLFCLKVFLPARPNDLENTGLS
                     EPADYNWTLTDLDGQAVPFAKFKGKTVFLNIWATWCGPCVGEMPSIAKLARDPRLQGK
                     NIEFVCASIAESAESVRGFLTGKDWTMTILRADKTPAVFSSEGIPATFLIGPDGRIAA
                     TKIGAADWNEPGVVAFLEQLASTPTKPR"
     gene            complement(4968..>7590)
                     /locus_tag="VHS97_01390"
     CDS             complement(4968..>7590)
                     /locus_tag="VHS97_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX3446872.1"
                     /translation="AKMAVEQARKAGSAIGDVYFWGAMGSIAGTFLCGFVLMNLAPTS
                     TIVVLVAAALALLAAALIGEPLGLAAGIAAALLLGLGSVAPLVSRLNLGAIDLGSYKL
                     NYLAVAGTFAGIILGILGVVGLFRARSEDQALTAAVAAKSPGGDFGVDKPRPKLTDLA
                     VLAFAASMAFMALEMVAGRLVQRHLGSSIYGWTSVIGVLLAGLSFGNFLGGKIADFVK
                     NEKQASWLFLAASILSLTVLLTEKQPQWFADHALGGEGKALLSQAITLSELKLGNYSV
                     PMNWQYRILFVTTLVFFLPALSLGTVSPVVAKLAVDRLKRYKRTGTAIGQVYAWGMVG
                     SIVGTFLTGFFLIDILGTKGVILLLGTTMTFGATLLGTVWHAVWAGIPLGLCVLAFTP
                     PAFVDAIGKVFPYVKGAAFEDIGKRWGIREQLGNPNDPLSEFVWVDESNYYYIKVENQ
                     IEDEGAIQKRTLVLDNLIHGYFILDHPERLDYDYEHVYALVAYRAAQASGKVKFKPAA
                     ANNGPATPPPPGASTSSTKDDSRRIAPAKDDEAKPSPKKDTTGAPAKDEAAKPPGGLK
                     SVQASSDDGKITPPKDGESKPKIDRSDPLDLEELGSTVPAVEQSSMNTLFLGGGAYCF
                     QRHMQYAYPGTGVDVAEIDRAVTNANWLATGLPKDSPIKTHWGDARQFVELNQDSKQY
                     DLVFGDAFNDFSVPWHLTTREFNDKIKKMLTPNGVYMINIIDVYESDEHAQESAQKKI
                     DDQKITDPAEQTRIREEALARAHRYGGFVGAWTKTARLTWDNVYIFGTDNTPGGGLRE
                     TFVVVASMKPLDLEDLGRRPLDPKFYTKSTRTEPKPYGPSDVQAVDVRSRNIILTDDY
                     APVENLLGPVAETRADE"
BASE COUNT         1358 a         2209 c         2462 g         1561 t
ORIGIN      
        1 aacaagtcgc ggggcgagct tgaatgggac ctcggtgttt gccgcgatgc tgcggagtga
       61 aaggcatgaa ctgcgcacgg cgatcaggct cagcgacgag cttgcggaat gggccgacgg
      121 aatcgtccgt tttgccccat atcccggtcc tcccgatcgt gaagaggcag agtggtacga
      181 acaatggctc gccgatgatg cggaccgttg gttgatctat gtggtacgtg acttcgacgc
      241 ggtcgcagag tactggaaaa ccgtggcgga ccgtctgccc gccgccaacg aaccggaacg
      301 gagggcggaa gccgaggaac gccgatcgaa aggggccaac tgggtcagcc ggctccccaa
      361 taaagcgaag aaggcggccg atccgtatgc gtggttcgag gtggataccg gctggaaccc
      421 gccgcgcacg tgcaccaact tgaccggtga atgggccggg gggatcgatg gcgtggcggc
      481 cggcttgacg gtgcatgaag cgatccgatc tcgcagctgc cgtctcttgc ttgaagccga
      541 cggcaagccg ttcgtaatgg aaaaaacagt cggcgcagga cgagtacttg tagtcgccaa
      601 tggatcgttc ctgctgaacg agggagttgc caatcccgcg cgccggccgc tggccgagcg
      661 cgtgttggat tgggtcaacg gccggaccgc gcgtgttgcc atggtcgatg gtccttcggt
      721 gctgggagaa ggcgaaggga tgccctcgct ctgggatttg ctctggcgca ttcctccctt
      781 gcgctgggtg gcgatacaag tgggtttggc cgcgtttttg gcggccctgg cgcgcgcacc
      841 tcgtctcggc cgtccgcgcc ctgatcctcc atcgggggct gaccggcccg ctgagcatgc
      901 ggtggcactg ggggcgctcc tggcgaagac cggagcggca cgcgaggcta atgaactggt
      961 cgattcttac cggcgatggc ggacgcctca cccgaggaaa cctggtctcc atccctaagg
     1021 cggtgctgga atggatgata atgagctcgc ttctggagct tcgtcgcaac cgggccagca
     1081 gcaagttggg gaggacgccg ccggcctatc ggacggccgc cgcggcccca acgaactggt
     1141 gagctggtcg cggcgcgtgc tcggcgaagt caacaaggtg ttcgtcggtc agccgacact
     1201 ggtgcgcggc gtcttggcgt cgctcttggc cgagggacat gtcttgatcg aaagcgtgcc
     1261 ggggctgggc aagactctcc tggtgcgagt tctggggcag gtcttgggtt gctcgttcaa
     1321 tcgcatccag ttcacgccgg acctgatgcc ctcggacgtg accggctcgc caatttacga
     1381 cgaacggatc aacgactttc ggttccgtcc cggaccggtc ttcacgcaaa tcttgctcgc
     1441 cgacgagatc aatcgggcac cggccaagac gcattcggcg ctgctcgaga tcatgcaaga
     1501 gaatcgggtg accatcgacg gcgtcagcta tccgatcgag ccgccgtttc tggtcatggc
     1561 cacccagaac cctatcgaat cggagggaac ttacaatctt cccgaggctc agctcgatcg
     1621 gtttctgttc aagttggtgg ttgcctatcc gtcggcgtcc gaagagaccg acattctcaa
     1681 gttgcacacg cggggtttcg gcatcgaccg aggaatagcc gaccgcttga gcgtggtgac
     1741 gagtccggcc gaggtgcgcg ccatgcagag gcgggctgag agcgtgctgg ttgacgacca
     1801 tgttctggga tacatcgcca ccctggtgcg caagacccgt cagtggccga cgttttcgct
     1861 aggagcctct ccgcgcgcgg gggtggcgat cctgcggggc gcgcgggcgg tggcggcaat
     1921 ggaggggcgc gaatacattc tgccagatga cgttcaagac gtgctcctgc cagccttgcg
     1981 gcatcgagtg ctgctcacac ccgaggccga ggtcgagggc cagtcagccg acgaactctt
     2041 gaccgagctg atcagatccg tcgaggtgcc gctgcgatga agatggtctg gccgggtcga
     2101 gcgctggggg tcgcactctt ggtaccggcg ctcttatcct tggtgctttt catccgtgaa
     2161 gaggtcttgc cgctggtgct ggcgatcgat gggatcgtta gtttcgtggc gctcatcgat
     2221 ttggccagcc tggtaggggc gggccgtttg cgggtcgagc gccaggtggg ccgggtctgt
     2281 tcactcaacg agcaccagga cgttgttctc tcgatcgaaa acgtgggtcg gcgcgcgcgg
     2341 tcgctacgat tgcgcgacga cgttcccgac gaattctcag cagatccggc ggcgtttgac
     2401 gtcacggtgc caagcaggcg ccaggccagc ctcacgtacc gcttcgtgcc caagcggcgc
     2461 gggacctatg tgttccagca ggtcgacgcg ctcgtcgaca gccgcctggg gttttggcga
     2521 gggcaatact cctggccact acggaccgag gttcgggttt accccaacat tcatcaggtc
     2581 gcgaggttca cgatgctggc gcggcgcgat cggctttcga ccatggggct gcggcggtcg
     2641 cgccggctgg gcacggacaa cgagtttgaa cggctgcgcg actacatcga gggagacgat
     2701 cctcgtcacc tggactggcg tgcgacggca cggcggcgga agcttacatg ccgggcattc
     2761 caggccaacc agagccagcg gatcatgttc ttgatcgatt gcggccggct gatggccggc
     2821 gacaccggcg atggtctttc gcccttggac catgcgttca acgccatgct gcttttggct
     2881 catgtcgccc tgatccgggg cgaccaggtg ggtatgctgg cgttttcgga ccgggtctgc
     2941 gcctacgtgg cgcccggcgg cggaccgcgc cggatcaatc gtctggtgca tggcgtgcat
     3001 aacatttttc ctgagatggt cgagcctcgt tacgatcggg cgttcatcga gcttgagcaa
     3061 cgatgtcgca agcgctcgct ggtcgtgctt atgacgaacc tgttcgacga gcttagtgcg
     3121 gagcttgccg gtgagtatct ccgcaatcta tcgggccgtc atttaccgct cggagtcttt
     3181 ctccgcgacc acgatctctt tgctatggcc gacagggccg cggccggcgg ggcggggctc
     3241 tatcaagggg ccgcggccgc gtcgctcttg aactggcgcg aacgtgtcct ggccgggctg
     3301 cggatgagcg gggtcttgac cctcgacgtg tttcccgagg aattaacggc cgtcctggtc
     3361 aaccgctaca tcgaaatcaa agcgcgacac ctgctctgac acaattaccg accgcgcaaa
     3421 aagctgccgg agatcgaccc atggactcat tccagccccc gagcgatcac gatcgcgaga
     3481 atccctatgc tccgccccaa tccgcgttgc tgccccgggc gagggattgg tcggcggcgg
     3541 gcgtgccgtt cacggtgggg gacgtgtttg aacgaagctg gtcgatcttc aagaccaggt
     3601 tttggtcctg cctatcggtc gtctggggcg tcgtgggcat caactgggcc ataagcttcg
     3661 gaatgaacgt gttgctggcc ggcctcgccg agatcgcgcg cgacgagacg gtgtaccgtc
     3721 tgctctacgt tttgacgttg tttggcgcca tcgtgctcca ggtttgggtc gcttgggtcg
     3781 gccagaccat tgcacttctc aagatcgtcc gcggcgagcc ggtcgcgttt gaagatgtct
     3841 tcaagggcgg tcgaatggtg ctgacgacca tactcgcatc gatcgtcatg cttgcggtga
     3901 tcgcggtgcc gatcttgatt gcggtgggcg cgatcaccgc gggcctggtc ttgatgcaaa
     3961 accagtcggg cttgggcgcc gtgctcttgt ttctggttgt aagtgtaccc atcggagttg
     4021 ttgttgtctt tcttaccgtc cggctgtaca tgtactacta catggtgatc gaccgcgacg
     4081 ccggagtctt cgagtctttt cgccaatcct ggcaatgcac caagtatcag gctagtacca
     4141 taaccctcgt cttttgtctc gagcttgcga ttgtgctcgc cggctttctg gcgctctgcg
     4201 tcgggctgat tttcgcgata ccgctggcga atttgctggt cccggtcacc tatcagtttc
     4261 tgacaggaac agggaagtcc ggcggcacgg agcctgagtt ttactgggaa aatgaaaact
     4321 gacggacgat cgattagcgc ggcttggtcg gcgtgctcgc gagctgttcg agaaatgcca
     4381 cgacccctgg ctcgttccag tcagccgcgc cgatcttggt ggccgcgatc cggccgtcag
     4441 gaccgatcag aaacgtggcg ggaatgccct cggaagagaa aaccgcgggc gtcttgtcgg
     4501 cgcggaggat cgtcatggtc caatccttgc ccgtcaggaa cccgcgcacg gactcggcgg
     4561 actcggcgat ggacgcgcag acgaattcga tattcttacc ctgaagccgc ggatctcgtg
     4621 ccagcttggc gatcgagggc atttctccca cacacggtcc gcaccaagtc gcccagatgt
     4681 tgaggaacac cgtcttgccc ttgaacttcg cgaatggtac ggcctgaccg tccaagtccg
     4741 tgagtgtcca gttgtaatcg gccggctcgc tcaagccggt gttttcgagg tcattgggac
     4801 gagcgggaag gaagaccttc aggcaaaaca accagaatag cgccaacgca atggcgaggt
     4861 acatcaacgt tcggttgggc ggtgagtgtg cttcggtcat cgatgggtct cgacctgtga
     4921 gaaggtcgcc aagctagata ggattccggc cggcgaacct ctgtcgttca ttcgtcggcg
     4981 cgggtctcgg cgacaggtcc cagcaagttt tccacgggag catagtcgtc ggtcaaaatg
     5041 atgttgcgtg agcggacatc cacggcctgg acgtcgctcg gcccgtacgg cttgggttcg
     5101 gtgcgcgtgc tcttggtgta aaacttggga tcgagaggcc ggcggcccaa gtcttccagg
     5161 tcgagcggct tcatggaagc aacgaccacg aacgtttcgc gtaagccccc accgggcgta
     5221 ttgtcggtgc cgaaaatata aacgttgtcc caagtgagcc tggcggtttt ggtccaggcg
     5281 cctacgaagc cgccgtagcg gtgagcgcgc gccagcgctt cctcgcggat cctggtctgc
     5341 tccgcagggt cagtaatctt ttggtcgtcg attttcttct gggcggactc ttgggcgtgc
     5401 tcgtcggact catagacgtc gatgatgttg atcatgtaca cgccgttggg cgtgagcatt
     5461 tttttgatct tgtcgttgaa ctcgcgcgtg gtcaggtgcc aggggacgga gaagtcgttg
     5521 aaggcgtcgc cgaagaccaa gtcgtattgc ttggaatcct ggttcagctc gacgaactgc
     5581 cgcgcgtcac cccaatgagt cttgatcggc gaatccttgg gaagcccggt ggccagccag
     5641 ttggcattcg taacggccct gtcgatctcg gcgacgtcga cgccggttcc cggataggca
     5701 tattgcatgt gtcgctggaa gcaatacgcc cccccgccca ggaaaagtgt attcatactc
     5761 gattgctcga ccgcggggac cgtggagcct agctcctcga gatcgagtgg atcggatcga
     5821 tcgatctttg gcttgctttc gccgtctttc ggaggcgtga tcttgccgtc gtcactcgat
     5881 gcctgcacgc tcttgagtcc gccgggtggt ttggcggctt cgtccttggc tggcgctcca
     5941 gtcgtgtctt ttttggggga cggcttggct tcgtcgtcct tggccggggc tattcgacgt
     6001 gaatcgtctt tcgtcgagct cgtgctggcg cctggaggtg gcggcgttgc aggtccattg
     6061 tttgccgccg ccggcttgaa tttcaccttg ccgctggcct gggctgcccg ataggccacc
     6121 agggcgtaga cgtgctcgta gtcgtagtcg agtcgttcgg ggtggtcgag gatgaaataa
     6181 ccgtgaatca ggttgtcgag caccaacgtt cgcttctgga tcgcgccctc gtcttcgatc
     6241 tggttttcga ccttgatgta atagtaattg ctctcatcga cccaaacgaa ttcactcaag
     6301 ggatcattgg gattgccgag ttgttcgcgg ataccccacc gcttgccaat atcctcaaaa
     6361 gccgccccct tcacgtaagg gaagaccttg ccaatcgcat cgacgaaggc cggcggggta
     6421 aacgccagga cgcacagacc cagggggatg ccagcccaga cagcgtgcca gacggtacct
     6481 agtagggtgg cgccgaaggt catcgtcgtt cccagcagca ggattacccc cttggtcccg
     6541 aggatgtcga tcaagaaaaa gcccgtcagg aaagtgccga cgatgctgcc gaccatgccc
     6601 caggcataga cctgaccgat ggcggtgcct gtgcgtttgt agcgtttcaa gcgatcgaca
     6661 gccagcttgg ccacgacggg gctcacggtt cccagcgaca gagccggaag aaagaagacg
     6721 agcgtcgtga caaagagtat tcggtactgc cagttcatcg gcacggagta gttgcccagt
     6781 ttcagttctg agagcgtgat ggcctggctc aagagagcct tcccttcgcc acccagggca
     6841 tggtcggcga accactgcgg ttgtttctcg gtcaagagga cggtgagcga gagaatcgac
     6901 gccgcgagga acagccagct cgcctgtttt tcattcttaa cgaagtcggc gatctttccg
     6961 cccaggaagt tgccgaatga caggccagca agcaaaacgc cgatgacgct ggtccagccg
     7021 tagatgctcg agcccaggtg ccgttggacc aagcggccgg caaccatctc gagggccatg
     7081 aacgccatac tcgccgcgaa ggcaaggacc gcgagatcgg tcagtttggg gcgcggcttg
     7141 tcgacaccga aatcgccgcc gggcgatttg gcggcgacag ccgccgtgag cgcctggtcc
     7201 tcgctacggg cgcggaagag cccgactacc cccaagattc ccaggataat ccccgcgaat
     7261 gtgccggcca cagcgaggta attcaacttg taagagccca gatcaatggc accgagattc
     7321 agccggctaa ccagaggcgc aaccgagcca agtcccagca agagggccgc agcgattccg
     7381 gcagccagtc ccaacggttc gccgatcagg gcggccgcca agagcgccag ggcggcggcg
     7441 accaggacga cgatcgttga cgtcggcgcc agattcatga gcacgaaccc gcagagaaac
     7501 gtgccggcga tcgagcccat cgcgccccaa aaatatacgt cgccaatcgc gctgccggct
     7561 ttccgagcct gctcgacggc catcttggcc
//
LOCUS       DATAQL010000082         4876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3977439, whole genome shotgun sequence.
ACCESSION   DATAQL010000082 DATAQL010000000
VERSION     DATAQL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3977439"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            483..776
                     /locus_tag="VHS97_01395"
     CDS             483..776
                     /locus_tag="VHS97_01395"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX3446873.1"
                     /translation="MPTYMSLLNFTDQGIRNIKESPKRLASTKKALKKLGGELKAFYL
                     TQGNFDGVLIFEVPTEEALTTFLLTTGAAGNVRTTTLRAYPEDEYLKHIAALS"
     gene            complement(1327..2016)
                     /locus_tag="VHS97_01400"
     CDS             complement(1327..2016)
                     /locus_tag="VHS97_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX3446874.1"
                     /translation="MRVLATPAGRVNIVVDQASGQQRVGRFGWKCQQATLFTFSGDAY
                     LNEMGITTPLFPAENCPQGKCGLLNNPALKPAPNDSDNSSLVEFTDFMTFLAPVPQGN
                     FINQARFGGFVFREIGCINCQAPVLRTGSNGSAALNEVTFFPFSDFLLHDMGSLNDGI
                     AQSGAGQNEIRTAPLWGCRLLTTFLHDGRANNLSDAILAHDGQGRDARNRFAGLNDDP
                     KAALLAFLNAL"
     gene            complement(2048..3352)
                     /locus_tag="VHS97_01405"
     CDS             complement(2048..3352)
                     /locus_tag="VHS97_01405"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX3446875.1"
                     /translation="MNAIMRMMRVRKPISAALGAAILMLGFAALGGGSGTNFGDPLPG
                     LTPDLQARFAAGLANFQSPDDPTTGLGPVFNNTPCSSCHSTGAVGGGSNLVETRYGTV
                     TNGVFDPLAQYGGSLIHAQGIGSSFNGVPFTTPNGTVVNFVGEIVPQPPAVPNGPATI
                     VAGRRTTPLFGLGLVDAVSDDTFQNLANLERFLSPETAGRVNVVSDVFADGQDPQARA
                     GRFGWKAQHSTLFAFAGDAYLNEIGITTPLFPNENVPQDPNEGRLVNPALLAANPGPA
                     VGPDNTNDPDNASLSQFTDFMTFLAPPPKLALTDQARSGAQTFFAIGCANCHVPTLTT
                     GPNSIPQLDQVRFHPFSDFLVHDMGSLGDGIAQGGVGQTEMRTAPLWGVRILPSFLHD
                     GRASTLSEAILFHAGQGQDARDRFANLEADDQANLIAFLNSL"
     gene            complement(3528..4787)
                     /locus_tag="VHS97_01410"
     CDS             complement(3528..4787)
                     /locus_tag="VHS97_01410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3446876.1"
                     /translation="MKPASDHATDLGSLTPDAALALVEVLAAEMIQRWRQGEHILPEA
                     YLDRFPGLREHPEAATELIYEEINLRSEYGLDVPTEQVLERFAQWRPQLEVLLDCHRI
                     LSPRPAPRFPRAGEVIDDFRLLAKLGSGRLGRVFLASQMSLADRHVVLKFTPCDALEY
                     LTLARLQHPHIVPLYSVHNHPDCGLRSLCMPYHGGVVLSRLFALMRPTPPAKRTAQTV
                     LDALDRAQAQARPAGPPDRIVQPASARGSYAQAVCWIGACLADALHYAHERNLVHLDI
                     KPSNVLMAASGQPMLLDFHLAREPVGPGRERPYVFGGTPGYMSPEHRDALQAIRESRP
                     IERPVDARSDVYSLGVTLYEALTGELPAKGDDLKSVRRHNASVTTSLDDCITACLADE
                     PARRHRDASALSVLLRQHAGDCVLGTY"
BASE COUNT          961 a         1422 c         1594 g          899 t
ORIGIN      
        1 cttgaacagt tgcgggagct gcgtagagaa tctttcctcg gtttcgagaa ctaagaagtg
       61 tccgccgcca atgactttgt gaatgtacgt taccttgcct tccgagtcga tcttgcacac
      121 accctggccg gtgtggatga agaacactcc cccctcgcat tcacgacgat gccgaaagcc
      181 gggtgagcga aggcctcgtc agtgaacagc ccgcacagag acgaaacaac caggactgcg
      241 ccgatgcacg aacggcccat gatcgcacat ccagttcttc gtgtgcggtc gggtgccaaa
      301 gcatgttgtc agcatgtcca ttctacgaac gccaactcga cgttgccctg gtcgaattgg
      361 gggaacaccc ttgcctgctt cctcgggccc gtctaacatc aggatttgct gatggtcacg
      421 tctgtgcgag attctgattg cccatcgatt cgatgtatca acattccttg cggggggtcc
      481 caatgccaac ctacatgagt ttgcttaact tcaccgacca gggaatccgc aacatcaagg
      541 aatcgcccaa gcggctggcg tcaaccaaga aggccttgaa aaagctggga ggggagttga
      601 aagccttcta cctgacccag ggcaacttcg acggagtgct gatcttcgag gtgcccaccg
      661 aagaggcgct cacgactttc ctgctcacca ccggcgccgc cgggaacgtg cggaccacaa
      721 cactccgggc gtaccccgaa gacgaatacc tgaagcacat cgcggcgctg tcttgactct
      781 gagcggcccg gccagcccgc tcattccacg cgccgaggcc ggcccggaca cttgccgtca
      841 tcacgaccgg cggctcttga ctttaatcgg tacatctgtc gagatgcctg tgtcccgacc
      901 tgcccgcgaa gttgaaggcg aagccatcga agatcagaac ggccgttctg attggctgga
      961 tccgtagacc acgtcggcac ctccaaaacc gagtcggttc ccgccaaaga aagaaagacc
     1021 cactgctttt ccagcgcgac tgcgtttata ctggacattg aatttcttcc ttgacagggg
     1081 ggattttcac cgggccttga tcggaatacc cgccgatcga ggcccacgtt gtttgggggt
     1141 ggagtggggc tttgaactac gaacggctcg tgttagatcg tgccgccaaa tcgaggaagg
     1201 gggccaggac caaccaagcc ccccagagtt gatccgaccc tggagtcgtc ggtcagaccc
     1261 ggtctcaagc cattcaagaa tgaacttgaa accaagtctg aactgtttcg cttccgcatg
     1321 cggaatttac agcgcgttca ggaaggcgag cagcgccgcc ttcgggtcat cgttgaggcc
     1381 ggcgaagcga ttgcgagcgt cgcggccctg gccgtcgtgc gccaggatgg cgtcggagag
     1441 attgttggcg cggccgtcgt gcaggaaggt cgtcagcaga cggcagcccc agagcggggc
     1501 ggtccgaatc tcgttctggc cggcgccgct ttgcgcgatc ccgtcgttca ggctgcccat
     1561 gtcatgaagc aggaagtccg aaaacgggaa gaaggtcacc tcattcaagg ccgcggaccc
     1621 gttacttccg gtccgcaaga ccggcgcttg gcaattgatg caaccgatct cgcggaacac
     1681 gaagccgccg aacctcgcct gattgatgaa gtttccctgc ggaacggggg ccaggaacgt
     1741 catgaaatcc gtgaactcca ccagggagct gttatcggaa tcattgggcg ccggtttcaa
     1801 ggcggggttg ttcaagaggc cgcactttcc ctggggacag ttctccgccg ggaacagcgg
     1861 cgttgtgatc cccatttcat tgaggtatgc atcgccagag aacgtgaaga gcgttgcctg
     1921 ctggcacttc cagccgaacc gccccaccct ctgctggccg ctagcctggt ccacgacgat
     1981 gttgacgcgt ccggccgggg tcgccaggac acgcattgca ttgatcgcac tggatcatcg
     2041 agagatctca gagcgaattc aggaaggcga tcaggttggc ctggtcatcc gcctcaaggt
     2101 tggcgaaccg gtcacgagca tcctggcctt ggccggcgtg gaagaggatc gcctcggaga
     2161 gtgtgctcgc ccgaccgtcg tggaggaaac tcggcaagat gcggacgccc cagagcggtg
     2221 ccgtcctcat ctccgtctgg ccgaccccgc cctgtgcgat gccgtctccc aggctaccca
     2281 tgtcgtggac caggaagtcg gagaacgggt ggaacctgac ctggtcgagc tgggggatcg
     2341 agttcggtcc ggtcgtcaac gtggggacgt gacagttggc acagccgatc gcaaagaacg
     2401 tctgggcccc gctccgggcc tggtcggtca gcgccaactt gggtgggggt gccaagaacg
     2461 tcatgaagtc ggtgaactgc gaaagggacg cgttgtccgg gtcattcgtg ttatccggac
     2521 cgacagccgg acccgggttg gccgccagca gagcggggtt cacaaggcga ccttcattcg
     2581 gatcctgggg gacgttctcg ttgggaaata gcggggtggt gatccctatc tcgttgagat
     2641 aggcgtcgcc ggcaaacgcg aacagcgtgc tgtgctgggc cttccagccg aaccggcccg
     2701 cgcgcgcttg ggggtcctgc ccgtcggcga agacgtcgct gacaacgttg acgcggccgg
     2761 cggtctccgg cgagaggaac cgctcgagat tggcaaggtt ttggaaggtg tcatccgaca
     2821 cggcgtcgac caacccgagg ccgaacagcg gagtggtccg ccgaccggcg acgatcgtcg
     2881 ccggaccgtt gggaacggcc gggggctgcg ggacgatttc tccgacgaag ttgaccacgg
     2941 tcccgtttgg tgtcgtgaag gggaccccgt tgaagctgct gccgatcccc tgggcgtgga
     3001 tgagcgaacc gccgtactgg gcgagcggat cgaacacacc gttggtcacc gtcccgtacc
     3061 gggtctcaac gaggttgctg cccccgccga cggcaccggt gctgtgacaa gaagagcacg
     3121 gggtgttgtt gaagacgggc ccgagcccgg tcgtggggtc gtcgggggac tggaaattgg
     3181 ccaggcccgc ggcgaagcga gcttgcaagt cgggggtgag ccccggcagc gggtcgccaa
     3241 agttggtacc tgagccgccc cctaaggccg cgaagccgag catgagaata gccgcgccga
     3301 gcgcggcgga aatcggtttg cgcactctca tcattctcat aatcgcattc atgagtgccg
     3361 tcctggtttg gtagatgggg tgatgcttga tcccgttttg catgtcgatg aacgagctat
     3421 tcaaaaggtg aatgccgcag aggaacgcag gagcctatag gagcccccaa gcaagaggct
     3481 caacggaaat tcggcgtcga ggcgggcgcg aaatttttga agacagatta atacgttccg
     3541 agaacgcagt cccccgcgtg ctgccgaagt agcaccgaga gtgccgatgc gtctcggtgc
     3601 cggcgggctg gttcatcggc caggcaggct gtaatgcagt cgtcgaggga ggtagtcacg
     3661 ctggcgttat ggcggcgcac cgatttcagg tcgtcgccct tggcaggcaa ctctcccgtg
     3721 agggcctcat agagggtgac acccagagaa taaacgtcag aacgtgcgtc gacgggtcgc
     3781 tcgattgggc ggctttcccg gatcgcttgc aacgcatctc tgtgttcggg agacatgtaa
     3841 ccgggcgtcc cgccgaaaac atacggtctt tcgcggccgg ggccgaccgg ctctcgtgcc
     3901 aggtggaaat ccaggagcat cggctgcccg ctggctgcca tgagcacgtt ggatggcttg
     3961 atgtcgaggt ggacgaggtt tcgctcatga gcatagtgaa gagcgtcggc caggcaagcg
     4021 ccgatccaac acacggcttg agcgtaggag ccgcgcgcgg acgcgggctg gacgattctg
     4081 tcgggcggcc cggccggtcg ggcttgcgcc tgggctcgat ccaaggcatc gagcaccgtt
     4141 tgcgctgtgc gctttgcggg tggagttggt ctcatcaagg cgaagagccg cgacagaacc
     4201 acgccgccgt gatacggcat gcacagcgag cgcaacccgc aatccgggtg gttgtggacc
     4261 gagtaaagcg ggacgatgtg aggatgctgc aaccgcgcca gggtgagata ctcgagggca
     4321 tcgcacggcg tgaatttcaa aacgacatgg cgatcggcta gcgacatctg gctcgccaga
     4381 aagacgcggc ccagccgccc gctgccgagc ttggcgagga gacggaagtc gtcgatgacc
     4441 tcaccggccc ggggaaatcg tggggcgggc cggggagaca ggatgcgatg gcaatctaag
     4501 agtacttcga gctgaggccg ccactgcgcg aatcgctcga gcacctgctc tgtcggcaca
     4561 tcgaggccgt attcggagcg gagattgatt tcctcgtaga tcagctctgt ggcggcttcg
     4621 ggatgttcgc gtaggccggg aaaccggtcg agataagcct cgggcaggat gtgctccccc
     4681 tggcgccagc gctggatcat ttcggcggcc agcacctcca ccagggcaag ggcggcatct
     4741 ggcgtcaagg agccaagatc ggtcgcgtgg tctgaagccg gtttcacgac ggagcgcctc
     4801 tcgctacgta agttgacccc gattgcgcga aggcgacgtt gccggcgaga atgcggagaa
     4861 tccgccggac actgtc
//
LOCUS       DATAQL010000083         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1840176, whole genome shotgun sequence.
ACCESSION   DATAQL010000083 DATAQL010000000
VERSION     DATAQL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840176"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VHS97_01415"
     CDS             <1..457
                     /locus_tag="VHS97_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446877.1"
                     /translation="NDMIMMVQMFFAMHREHLSSAREELERVEQLTRELSALQARLRE
                     PTESPAAGPGAGPAAGVGRAAKRLPPLDAEDRQPRAVSGESNQAAHRRESGSPKSGIK
                     SSKRKSPPKSDGASELDSAQLHAQLTKRITELQRERQGYWQSIMSKMSK"
     gene            799..1656
                     /locus_tag="VHS97_01420"
     CDS             799..1656
                     /locus_tag="VHS97_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446878.1"
                     /translation="MSTDAKPSTSSSSPTPSTQHREPGIRIFMWPKVIFLYPTAIVAL
                     ICALGMSIIHDRTHDPTKSLKSAVEAKYLTQDNDSLTDEPGTSDKVERFRTPQNLLAM
                     LFLSMFAFNLLVMALDFPRFSLVAVILAILFALFFILWIGSYFHLDLLKPIHLIFSGI
                     YAVANKGFYIMVFVTLMLVFTVIYITRWLDYWEVLPNEILHHHGPLSDLERYPTMNLK
                     FDKEIPDVLEWALLGAGRLVLHVPNVSKALVLDNVLFISAKEKALKNVMSRLEVRVTT
                     DQEANESVT"
     gene            complement(1696..2571)
                     /locus_tag="VHS97_01425"
     CDS             complement(1696..2571)
                     /locus_tag="VHS97_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446879.1"
                     /translation="MHEPRGRHLPLAGPRRFIGDLVHFARRIPSAPVSRSFDVSALTD
                     PRVRHPARPSWSCLFMKAYALVGSEHAPLRRSLLEFPWPRLYEHPWMNCALAIERTFE
                     GEEGVFVGIFRAPERQTLTQLQQAVSWYKNETLEKVGFYRMALRFSKAPTPVRRLLWW
                     STLNLSGYKRCKRFGTFGLSSYGALGAEQIHPISPLTTTLTYGPIDPATGRVVVKLIY
                     DHRVLDGAYIARRLRDIEEVMHGPILAELRDDRAMAAGVTSAEPPPNVEPLLKPHLSR
                     GTAPADSHPTRDFAG"
BASE COUNT          595 a          838 c          830 g          568 t
ORIGIN      
        1 taacgacatg attatgatgg ttcagatgtt tttcgcgatg cacagggaac atctgtcgtc
       61 ggcgcgcgag gagctcgaga gagtcgagca actgacccgc gagctgagcg cgctgcaagc
      121 cagacttaga gagccaactg aatcgccggc cgccggtccg ggcgccgggc cggccgccgg
      181 ggtcggtcgc gctgccaagc gtctaccgcc tctcgacgcc gaggatcgtc agccgcgggc
      241 cgtctcgggt gaatcgaacc aagcggctca tcgacgcgaa tcgggttcgc ccaaatcggg
      301 aatcaaatct tccaagcgaa agtcaccacc gaaatcggat ggggcgagcg aactcgacag
      361 cgcccaactc cacgcccagt tgaccaaacg catcacggaa ctccagcgcg agcgacaggg
      421 atactggcaa tccatcatga gcaaaatgag caagtgatgg cccttcggat acgcccgcga
      481 cctaccgaac gccatgcacg gccgatcggc ccgccgccag tttcgcatcc acttgcagct
      541 cttgccggcg cgattattgc gatcgcccta tccaaggttt gagctgaggc ggcgaacatc
      601 ctgatggtga gatcggcaac gtggcgaccc tacgtcggtc gccgtcggag cgaatctcct
      661 agtcttcagg gactttaccc atgattgccg gggcgttagg atgacctcgc ccagtcattt
      721 ggcacacgct gtgcgtgtga cgatggtccg cagtcagcaa gaacttaagt cgacctttca
      781 gcgcggagtt tgccagtcat gtcaacggac gcgaagccat ccacttcctc ttcatcgcct
      841 acaccgtcca ctcagcatcg cgagccaggg atccggatct tcatgtggcc caaggtgatt
      901 tttttgtatc cgacagccat cgtcgccctg atctgcgcgc tggggatgtc gatcatacac
      961 gaccggactc acgatccgac caagtccctg aagtcagcag tggaagccaa gtatttaacg
     1021 caagataacg attccttgac ggatgagccc ggcacgtctg acaaggtcga acggttccga
     1081 acgccccaga atttactcgc gatgctcttt ctatccatgt tcgcgttcaa cctgctggtg
     1141 atggcgctcg actttccccg attctcgctt gtcgcggtga tcctcgcgat cctgtttgct
     1201 ctgttcttca tcctctggat cggctcgtat tttcatctcg atttgctcaa gccaatccat
     1261 ttgatcttca gcggcatcta cgctgtggca aacaagggtt tttacatcat ggtctttgtg
     1321 accttgatgc tggttttcac ggtcatttac atcactcgat ggctggacta ctgggaagtc
     1381 ttgcccaacg agatcctgca tcaccacggg ccgctctccg acctcgagcg ttatccgacc
     1441 atgaatctga agtttgacaa ggagattccc gacgtgcttg agtgggcact attgggcgca
     1501 ggccgactcg ttctgcacgt tcccaatgtg tccaaggcgc ttgtgctcga caacgtcttg
     1561 ttcatcagtg ccaaggaaaa agcgctcaag aacgtaatga gccgtctcga agtgcgtgtt
     1621 acgacggacc aggaggccaa cgagtcggtc acgtgagccg gacaaacctg attgattcgt
     1681 gcaaaacgga gaggttcagc ccgcaaagtc acgggttggg tgtgagtctg cgggcgcggt
     1741 tccgcgcgac aggtgaggtt tgagcagcgg ctcgacatta ggtggcggtt cggcggacgt
     1801 cacgccggcc gccatggccc gatcatcccg aagttccgca agaattggtc cgtgcatgac
     1861 ctcctcaatg tcgcgaagcc tgcgagcgat gtaggctccg tcgagcaccc gatggtcgta
     1921 gatcagcttg acgacaaccc ggccggtggc cgggtcgatc gggccgtagg tcaacgtggt
     1981 cgtcaacggt gagatggggt gaatttgctc ggcgcccaaa gccccgtagc tcgagaggcc
     2041 gaaggtgcca aaccgcttgc accgcttgta gcccgacagg ttcagtgtac tccaccaaag
     2101 tagccgccga accggcgtcg gtgccttact gaaccgcaat gccatacggt aaaagcccac
     2161 tttttcgagc gtctcgttct tgtaccacga cacggcctgt tggagttgag tgagcgtctg
     2221 acgctcgggc gcgcggaaaa tcccgacgaa gaccccttcc tccccctcga aggtgcgctc
     2281 gatcgccagc gcgcaattca tccatgggtg ctcgtacaac cggggccagg gaaactcgag
     2341 caaagaccgc cgcaagggcg cgtgttccga gccgaccagc gcgtaggcct tcatgaacag
     2401 acacgaccat gaaggccgtg ccgggtgcct cacgcgtgga tcggtaagtg cgctcacatc
     2461 gaacgatcgg ctgaccggcg ccgaagggat tcgacgcgcg aaatgcacga gatcgccgat
     2521 gaaacgccgt ggccccgcga gcggtagatg acggcctctt ggttcgtgca tccatgcacc
     2581 tcgaggagtg gcgatcggcg caatgtcatt ggcgccgtcc agttagcata gttgggggct
     2641 cttgctcgac acgagttcat cgccacgagc aagcggtccg aagactacaa gaaatcgtgt
     2701 aggcggggaa ggcgaagctt tcaagaataa ggcaagaaca ggctcgaacg aacttccaaa
     2761 agggagtcgg cggcccgggc gccagacgtc tggctcgacc cgtgctgggt caggaatgga
     2821 tttcccagat c
//
LOCUS       DATAQL010000084         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_160742, whole genome shotgun sequence.
ACCESSION   DATAQL010000084 DATAQL010000000
VERSION     DATAQL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160742"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VHS97_01430"
     CDS             complement(<1..518)
                     /locus_tag="VHS97_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446880.1"
                     /translation="MYDERDDGKEPELKAGETIGTATPSVAPREASLFEPVRDRYGWR
                     LALVFGNHARGGRCPYFPHACFHCDIGAGEETAFDRAANRQRLDWFRDYYQAQLGSIN
                     HLVLYNSGSILNPREMPPDLLDEILAFARSLGATRIVSVDSREAYIRQGALSRVVSVL
                     GSGIMLRPILGI"
     gene            complement(499..>1695)
                     /locus_tag="VHS97_01435"
     CDS             complement(499..>1695)
                     /locus_tag="VHS97_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3446881.1"
                     /translation="LWERVAVFLGFTEFDACKVMGLAAYGDPEHYRPQFDRLFPLIEP
                     DEGRCGQGRLPFGINPVLARFRARDVHGLESLFGPRRRSDDPPELDRFADVAAGLQRC
                     TNLAVLAIARRLARSAGERSLVYAGGVALNCVSNSSLEREGPFDSLFVPGAAHDAGTA
                     VGAALAVAHEDDGLRHEPMRMGPRARSPFLGLSYDHPAIDAAIFRSGVVAEIVANPAI
                     TAASLVADGQVVGWFQGRDEFGPRALGGRSLLADPRRAEMRAALNQRIKHRESFRPFG
                     ASVLAEDAADWFLIPGDRPGAACCRQFMILAYPVRPARADLIPAVLHHDGTCRLQVVD
                     AQTAPLFHTLITRFRELTGVPMVLNTSFNDQEPLVATPDDAIKTFSRTHIDVLILGDR
                     IVRRTR"
BASE COUNT          366 a          520 c          538 g          271 t
ORIGIN      
        1 tcaatgccga gaatcggacg taacatgatt ccagaaccaa gtaccgagac aactcgcgag
       61 agagcgccct gcctgatgta ggcctcacgc gaatcgacgg agacaatccg agtggcgcca
      121 agcgagcggg caaaggccag aatctcgtcg agcaggtccg gcggcatctc gcggggattg
      181 agtatcgagc cggagttgta cagcacgagg tggtttatcg aaccaagttg cgcttgatag
      241 tagtcgcgga accaatcgag ccgctggcgg ttggcggcac gatcaaaggc tgtctcctca
      301 ccagcaccga tgtcgcaatg gaaacacgcg tgcggaaagt aaggacagcg accgccccgg
      361 gcatggttgc cgaaaacgag tgccagccgc cacccatagc ggtcgcgcac tggttcgaaa
      421 agactcgctt cgcgcggcgc cacgctgggt gtcgcggtac cgatcgtctc cccagccttg
      481 agctccggct cttttccgtc atcgcgttcg tcgtacaatg cgatctccga gaatcaggac
      541 atcgatatga gttcgcgaga aagtcttgat cgcatcatcg ggcgtggcga ccagtggctc
      601 ctggtcgttg aacgacgtgt taaggaccat gggcacgccc gtgagctcgc ggaatctggt
      661 gatcaatgtg tggaacaatg gcgcagtctg ggcgtcgaca acctggagac gacatgtgcc
      721 gtcgtgatgg agcacagcgg gaatgagatc ggctcgcgcg ggacggacgg gataggccag
      781 aatcatgaac tgccggcaac aagcggctcc cggccgatcg cctgggatta aaaaccagtc
      841 ggcggcatct tcggccagaa ccgacgcgcc gaacggtcga aacgactcgc ggtgtttaat
      901 acgttgattg agcgcggcgc gcatctcggc gcggcgcgga tccgccagta gcgagcggcc
      961 acccagcgcc ctgggtccaa actcgtcgcg tccctgaaac cagccgacga cctggccgtc
     1021 cgcgaccaga gatgccgcgg ttatcgcggg attggcgacg atctcggcca ccactcccga
     1081 acgaaagatc gcggcatcga tcgccggatg atcataagag aggcccaaga aaggcgaacg
     1141 cgcacgaggt cccatgcgca ttggttcatg gcgaagccca tcgtcttcgt gcgcgacggc
     1201 gagggcagcc ccgaccgccg tgccggcatc gtgagctgcg ccaggcacga agagagagtc
     1261 aaacggccct tcgcgctcga gactagagtt ggacacacag ttgagggcga ccccgcccgc
     1321 gtagaccaag gacctttccc cggccgagcg agccaggcgt cgcgcgatcg caagaacggc
     1381 caggttagtg cacctttgca gcccggcggc aacatccgcg aaccgatcga gctcgggagg
     1441 atcatcggac ctccgcctcg ggccaaagag cgactccaga ccgtgcacat ccctcgcgcg
     1501 gaaccgtgcc aagacgggat tgatgccaaa tggcagtcgg ccctggccgc atcgtccttc
     1561 gtcgggttca atcagtggga agagccggtc aaactgcggg cgataatgtt ctgggtcgcc
     1621 gtacgcggcc agccccatca ccttgcaggc gtcgaattcc gtgaatccga ggaacacggc
     1681 cacccgttcc cagag
//
LOCUS       DATAQL010000085         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2297813, whole genome shotgun sequence.
ACCESSION   DATAQL010000085 DATAQL010000000
VERSION     DATAQL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297813"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VHS97_01440"
     CDS             <1..694
                     /locus_tag="VHS97_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3446882.1"
                     /translation="FSKISTFLCPSDASQASVSVTTGTFTGTLPLTNYPNNIGTFAPE
                     ATTGMVNGPAYYVGVTSPSSSLVRLATITDGTSNTAIFSEYLAYESSTQNGNWQIYQD
                     TVDSAKVATPLLTLATDCQAAVTIAPPATGVSSDDGMKGVDWLFQHCGAGGCYSHVIT
                     PNKKACYFTGSKTAGHPTSTMIGASSFHPGGANVAFLDGSVRFIKDSVSQPTWWALAT
                     KAGGEVISSDSY"
     gene            807..1673
                     /locus_tag="VHS97_01445"
     CDS             807..1673
                     /locus_tag="VHS97_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3446883.1"
                     /translation="MNRRPEYSRRSFFGLTASAAALVPLAGHISITEAAYPGSGDLPW
                     LGLKVGLASYTFNKLPLDAAIKGILRVGVNYVSIKEAHLPLKSTALERKAVAQKFRDA
                     GITPLSCGVINMTDDESQLRNAFEYARDAGIPTIVCKPTHQSLPVLDTLVKQFGLRLA
                     IHNHGPEDKVWPSPLDVWAAIQKYDERIGVCIDVGHTARCGVDPVGAIRTCAPRLYDL
                     HIKDIASKTGKSMPVEVGRGVLDVRGMLKALLDIGYGYHVGLEYEKDMNDPIPGAAES
                     FGYIRATLAAMR"
     gene            1745..>3423
                     /locus_tag="VHS97_01450"
     CDS             1745..>3423
                     /locus_tag="VHS97_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3446884.1"
                     /translation="MTNRAIPLGILAFLLGTSSPGAVSADDVSSATADEPFTSAVRPI
                     LAARCFGCHAGKTPKAGLDLSRFDAQDGVLAAFRLWEKVIQKVKSNEMPPEVAKPLTN
                     GQRGTLIDWHHRTFVGLEPRPGPNRPRRLTRTEYRNTLSDLFGIPLRRDRGELFFQAD
                     TGSIVEKLLPADPPGPSGFDNDSSVLSLGPSEFAKCLQIAEYVVDQLDSLPEPRQALF
                     SVGSTKATMPRERARAILSRFAGRAFRRPVADAELAPFLAVFDAAYASPGKDGVKEGP
                     VAPVQGGTSPLQDARFGKAIQEGFTAILVSPKFLYRLETARESRTPYRISDHELATRL
                     AYFLWSTMPDDELFRLATEGRLHQDDVLGRQVERMLADPKSIALAENFGGQWLGYAEL
                     ENPDRFRVSRSEESIKLLRSMYREPLYFFDDLVRSNRSLLELIDSRHTFLNPTLGYHY
                     RLKGYKRERLIKDGGYDWPDPLRRVALDDPNRGGVLGMAAALVATSAPERTSPIRRGV
                     WILDASLGQRPPDPPPNIPPLEEAGNGQRLRSIKEQMEMHRGEVSCARCHDA"
BASE COUNT          652 a         1113 c         1030 g          628 t
ORIGIN      
        1 tttcagcaag atttcgactt ttctttgccc gtcggacgcg agccaggcgt cagtctcggt
       61 gacgaccggc acgttcacgg gaacgctgcc tctgaccaac tacccgaaca acatcggcac
      121 atttgccccg gaggcgacca cagggatggt gaacgggccg gcctactatg tcggagtcac
      181 gtcaccatcg agcagcctcg tcaggctcgc cacgatcacc gacggcactt ccaacaccgc
      241 catcttcagc gaatacctgg cgtacgagag ctcgacgcag aatggcaact ggcagattta
      301 ccaggacacg gtcgactcgg ccaaagtcgc gacgccgctc ctgaccctgg cgacggactg
      361 tcaggcggcg gtcacgatcg cgcccccggc gaccggggtc tcttccgacg acggtatgaa
      421 aggggtcgac tggctctttc aacactgcgg cgcggggggc tgttacagcc acgtgattac
      481 gcccaacaaa aaggcctgct acttcaccgg gagcaagacg gccggccacc cgacttccac
      541 catgatcggc gccagctcgt tccaccctgg tggcgcgaac gtggcgttcc tcgacggctc
      601 cgtcagattt atcaaggaca gcgtcagcca gccgacctgg tgggcgctgg ccacaaaagc
      661 cggaggcgag gtcatctcgt ccgacagcta ttaaaaaaaa gtggatcgct ccggggcgct
      721 gactccagcg tcccggcgcc ggtatgatcg tgatctcctg tgaaaaccca ctgtcgctcg
      781 tacctcgcgc cccaaaggag ccagtaatga atcgtcggcc tgagtattcg cgccggagtt
      841 ttttcggtct gaccgcatcg gccgcggccc tcgttccact cgcaggacac atctcgatta
      901 ccgaagccgc ctaccccggc tcgggagacc taccttggct cggtctcaag gtaggactcg
      961 cctcttacac cttcaacaaa ctgccactgg acgccgccat taagggcatc ctccgggttg
     1021 gagtcaacta cgtttcgatc aaggaggcgc acctgccctt gaagagcact gcgcttgagc
     1081 gcaaggcggt cgcccaaaag ttccgcgacg caggtatcac acccctctcc tgcggggtga
     1141 tcaacatgac cgacgacgaa tcgcaacttc gcaacgcgtt cgagtacgcc cgcgacgcgg
     1201 gcataccgac gatcgtttgc aagccgactc atcagtcctt gccggtgctc gacacgctgg
     1261 tcaagcagtt tggcctgagg ctagctattc acaatcacgg gccggaagac aaggtctggc
     1321 catcgccact ggatgtctgg gcggccatcc agaagtatga cgagcggatc ggagtgtgca
     1381 tcgacgtcgg gcacacggca cgctgtggcg tagaccccgt gggggcgatc cgcacctgcg
     1441 cccctcgact gtatgaccta cacatcaagg acatcgcgag caaaacgggg aagtcgatgc
     1501 cggtggaggt cgggcggggc gtcttggacg ttcgggggat gctcaaggcg cttcttgaca
     1561 tcggttatgg ttaccatgtc ggccttgagt acgagaaaga catgaacgac ccgattcccg
     1621 gcgcggcgga gagcttcgga tacatccggg ccacgctggc ggcgatgcgt taaattcgac
     1681 agccccgacg cggaccgtct cggtcgcgag ttcgatccgc gagaccacac ggaattcaac
     1741 gtcaatgacg aaccgcgcga tccccctggg cattctggcc tttctcttgg gcaccagttc
     1801 gcccggcgcg gtctcggcag atgacgtgtc gtcggcgacg gcggacgagc cgtttacgtc
     1861 ggccgtccgg ccgatcctgg ccgctcgatg ctttggttgc catgccggga aaaccccgaa
     1921 agcaggactt gatctctcgc ggtttgacgc gcaggacggg gtgctcgcag cgtttcgact
     1981 atgggagaag gtgatccaga aggtcaaatc aaatgagatg ccgcccgaag tggccaagcc
     2041 gctgaccaac ggccagcggg ggacactgat cgactggcac caccgaacgt tcgttggcct
     2101 ggagcctcgc cctggcccca acagaccccg tcgcctcacg cggaccgagt atcgaaacac
     2161 gctgtcggac ctgttcggca tccccttgcg ccgggaccgt ggcgagcttt tcttccaggc
     2221 ggataccggg tccatcgtcg agaagttgct cccggccgac ccaccgggcc cgtctgggtt
     2281 cgataacgac tcatcggtcc tctccctcgg tccgtcggag ttcgccaagt gccttcagat
     2341 cgcggaatat gtcgtcgacc agctcgactc tctcccggaa ccacgccagg ctctcttctc
     2401 cgtggggtcg accaaggcga caatgcctcg tgagcgcgct cgcgcgatcc tctcacggtt
     2461 cgccggccgt gcctttcggc gcccggtcgc ggatgcggag ctcgctccgt tcctcgctgt
     2521 tttcgatgca gcgtatgctt cgccagggaa agacggggtg aaggagggac cggtcgcgcc
     2581 agtgcagggc gggacgtccc ccctccagga cgcgcgcttc ggcaaggcca tccaggaggg
     2641 cttcacggcc atcctcgtct ccccaaagtt tctctatcgg ctcgagacgg cccgagagtc
     2701 gcgcacgcct taccgcatca gcgatcacga actggcgact cggctcgctt actttctttg
     2761 gtcgacgatg ccggacgacg agctgttccg gctggccacg gaaggacgct tgcaccagga
     2821 tgacgtcttg ggccgccagg tcgaacggat gctcgccgat cccaagagca tcgccctcgc
     2881 cgagaatttc ggcggccagt ggctcggcta cgcggagttg gagaatcccg accggttccg
     2941 ggtctcgcgt tccgaagagt cgatcaagct cttgcgatcg atgtatcgcg agccgctata
     3001 cttcttcgac gacctggtgc gctcaaatcg cagcctgtta gagcttatcg attcgcggca
     3061 cacattcttg aacccgacac tgggatatca ctatcgcctg aaaggttata aacgtgagcg
     3121 gttgatcaaa gacgggggct acgactggcc tgacccccta cggcgggtcg cgcttgacga
     3181 cccgaaccga ggcggcgtat tgggcatggc cgccgcgctc gtagccacct ccgcccccga
     3241 acgaaccagt ccgatccggc gcggtgtctg gatcttggac gcgagtctcg gccagcgtcc
     3301 ccctgatccg ccgccaaaca tccctcctct agaagaggcc gggaatggcc agcgacttcg
     3361 gtcgatcaag gaacaaatgg agatgcatcg cggcgaagtg agctgcgccc gctgccatga
     3421 cgc
//
LOCUS       DATAQL010000086        11505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2450796, whole genome shotgun sequence.
ACCESSION   DATAQL010000086 DATAQL010000000
VERSION     DATAQL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450796"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1714
                     /locus_tag="VHS97_01455"
     CDS             128..1714
                     /locus_tag="VHS97_01455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3446885.1"
                     /translation="MTKFQHATRMGLLMAAAAVLLGWVLRHKETSFADGLRYIHQAEK
                     IQAATWRDGLVKGIDHPLHPLGIAAVHRVLGGTDPTSWQIAASVLCFTSAVLLVIPIY
                     LLTLELFGEKTAWLGCLLVMVNPIIGYVVVNVLSESTFLLWWSFGLWGSVRFLREGRF
                     FWLPLAIGFGALAYLTRPEGMLLPAALVLTLLVLPLSRATRINWPRWWRAIGFLLTGV
                     VLLVGPYIALKGSVGTKPGIARVLGLAPRSLSLALEREKPIPPEQTDFDTYRIATIRM
                     LKVFRAAVTPPLFTVALLGIVLAAPLAAKGRARAGLFLTIVLAASAVALVRLHATGGY
                     CTVRHGLVPGIILTLAAAYGLTWLIEKVSIPGRWLGQADLRLRPGPAIWALLIGGAVV
                     FPNIQSQGPPVPGPFSVYRETGHWIAENTTAQDKVLDLTDWSLFFSGRPGYIFANVYE
                     APADPSTRWLVVRAPHVNGHWHYSEVLRELIAGREPVALVPPKADLNQVQIRIYDRQA
                     PPPPQTATATSSRDSTTRQR"
     gene            2023..2607
                     /locus_tag="VHS97_01460"
     CDS             2023..2607
                     /locus_tag="VHS97_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446886.1"
                     /translation="MADATGGENGNHSRPPDNAGRGPRQRRQSRTGRRPKGALRPVGP
                     SSGRGPIRVRRIGGEDFEFIHPRGVEEAELDYEEGIELWKAGDPESARDAIRYALSAY
                     HDNIWIHVALGQIALVEFHDPALARGHFGYAFELANRALPQGFKGRLPRERPNNRPFY
                     EAIDGLIQSLEALGRRADVDSLKALASRLASGSP"
     gene            2914..3804
                     /gene="ispE"
                     /locus_tag="VHS97_01465"
     CDS             2914..3804
                     /gene="ispE"
                     /locus_tag="VHS97_01465"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX3446887.1"
                     /translation="MRGLMIVRPIAGGVEVLAPAKLNLFLEVLGRRPDGYHEIETLMV
                     AVSLYDTLTFTDDPSGAISLRCSDPALPVGSDNLVVLAAERLRASAGCHRGARIVLEK
                     TIPTEAGLAGGSSDAAATLAALDRLWDLQTPPAKLSALAGEIGSDVAFFAHAPAAVCR
                     GRGERVEPVALKEEYHFVLVCPPVGMSTVNVYRQVVPPERPQPIGPVLEVLAHGGPAD
                     LGRSLFNRLQFFAETLLPELTRVKDALASLGSLLDGSMMSGSGSAYFGLCRSRAAACQ
                     AADILEPLGLGWVRVVTCGP"
     gene            3836..4498
                     /locus_tag="VHS97_01470"
     CDS             3836..4498
                     /locus_tag="VHS97_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HEX3446888.1"
                     /translation="MEITEVRIKLMEDNSGSNERLQAFCSITFDDMFVIRDLKIIEGA
                     KGFFVAMPSRKLTDRCHNCGTKNHLRSRFCNQCGCRLDENRALRDADGRAKLHADIAH
                     PINSMCREKIQAAVLASYAEELERSKMPGYISRYDEYEVDDFDVQYEGHPNGPPMTAG
                     NEPPVRRAPLRGHTQHARGNQSVLRGPHVSARKESTPEGVSSDHHHHHHRGDSSSFGN
                     GL"
     gene            4691..5707
                     /locus_tag="VHS97_01475"
     CDS             4691..5707
                     /locus_tag="VHS97_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3446889.1"
                     /translation="MLRPQRRHRVSIADDRAGQALNDDLSTSHRQPRDDRQATTATAS
                     RSVWSRLKVWLARIFTLAALICLGFLTVLYFLQARMIFPGASTQGQREAQVGSRADTE
                     LVDLRTKAGERVAALFAPALTSDGRPDPRASVRPTIIYFYGNAMCLNYATPELDRFRR
                     MGLNVLIPDYVGYGMSGGTPSERGCQATADTAYDYLVSRRGVDPRQIIAAGWSLGGAV
                     AIDLASRRTVGGLIAFSTFTSTNDMARTIVPLKLPRWFFVHRFESLRKIPTIACPILL
                     GHGRLDSLVPFAMFERLAAAAKTPVTALVIEQADHNDFYDEGGQRIDNAIAKFVSEHF
                     RGEP"
     gene            complement(5741..7342)
                     /locus_tag="VHS97_01480"
     CDS             complement(5741..7342)
                     /locus_tag="VHS97_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3446890.1"
                     /translation="MEGIPGAVLAEDAPTRTYTESVHSGLATSYRDQAILLSRQGLYA
                     ESEAYSREALRLRPDDVDTLNELGAALWWQRRYVEAEAIYRQADQLQPNDYRILTNLG
                     LALYQQGRVDEAGDYYRRAIQIDLAAFDAHVNLGIVLSDQGKFDEADDWLTRASEQRP
                     DSAEALQNLGMNLCRMGKSPEAIHHYEEALRHRPDHPEMHRNLAYALLTCGDYKRGWR
                     EYEWRLKCDPHPGYQINQTLWNGDDLDGRTILLHVEQGFGDTLQFIRFAPLVKRRGGH
                     VLVICQTPLLQLVARCDGVDLAFDDSCYKPKCHFQAPLLSLPAIFGTTLDTLPAQIPY
                     LATDPILVDHWRSEISRAIGLEGGECADALDQSDRAGGTKPFLIGIAWQGNPGHSDDR
                     WRSFRLSHLAPVAELSGVRLISLQTNHGLDQLESDGRSVRVVELPGRRRRDFMETAAI
                     MMHLDLVITPDTAVAHLAGGLGRRVWVGLSTVGEWRWPADRDDSLWYPTARLFHQTTL
                     GDWDGVFRRMATELKRELRGIPRDA"
     gene            complement(7462..8781)
                     /locus_tag="VHS97_01485"
     CDS             complement(7462..8781)
                     /locus_tag="VHS97_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3446891.1"
                     /translation="MRSIFSKVMLWSLGTFVLSLVAYWAISRTLDRHGPSKGDPFPHF
                     LRMVEDDLRHAYETGGPDQLARQLRRLDYYLPGEHLLTDECGRDLASGADRSDLLRRG
                     HRPPGPPRLADGRMIFVGPPHDNRYRFISIVKPWFDRPNILPYYGSIVLVIALMGWIL
                     AAHLAAPLRRLRRLVDQFGRGDLSTRALSTRKDEIGELSRSFDEMAGRIETLLSAERR
                     LLQDVSHELRSPLTRLDVAVDLASTSDDPGEFLGRIKRDIGRLSILVNELLQLTRAEG
                     DPTAHHLEPVQLDLLLEGLFEDCEVEAWAKGCRIERESMPPLEIMGEPELLRRAFENV
                     VRNAVRHAPKGTTVEIGLDSAGDAARISVRDHGPGVPDELLAAIFEPFFRVERDRSRA
                     SGGIGLGLAIARRAVDLHRGRISARNAQPGLVVNIELPMAEVLAGSA"
     gene            complement(8783..9535)
                     /locus_tag="VHS97_01490"
     CDS             complement(8783..9535)
                     /locus_tag="VHS97_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3446892.1"
                     /translation="MAHGTNLGFRLEQGADAARSGMLVLLIDDDTELCELLQEFFARR
                     NIRLETACDGRRGLARALAGGHDLLLLDVMMPGLDGFELLRLLRRQSQVPVIMLTART
                     TQSDRVAGLDAGADDYLPKPFGTEELMARIRAVLRRAVRGPKESEMLQLGGIRIIPPA
                     REVLVQDRPVALTTVEYDILEFLAHSAGRIVSRDELTAALYQRRATKFDRTLDMHICN
                     LRKKLGYHGGSIRTVRGVGYLCRVGPDAPGET"
     gene            complement(9601..10272)
                     /locus_tag="VHS97_01495"
     CDS             complement(9601..10272)
                     /locus_tag="VHS97_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446893.1"
                     /translation="MTEYLNTLGNAIIDRSPEELIGAVAVALTISLAMAGLYCIGRRK
                     ITENLLPMIVLMIVANLVSMAVGMGYFRVAHKKMGYVERELQRRPPGGPGGSAEVAAR
                     WIFRTADSDHDGRLSEEEASLAAADFVRRTDPSGQGSINLRSLDTAIQATMFHGRDPD
                     HLPRPFGNRGPWNPEISGPGSRSDRLPPRMGPPSDDQLPDINQSKNPSEERIRLEPEK
                     THISD"
     gene            complement(10355..10993)
                     /locus_tag="VHS97_01500"
     CDS             complement(10355..10993)
                     /locus_tag="VHS97_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX3446894.1"
                     /translation="MRVLAGLMSSVLILNLASGATAQPPGPGQEVYSQFATGGDLVTR
                     MMEFDKDKDGKLTKSEVTDQRLHRLFARSDANNDGIVTKNELASLAAKEESKSRGRSF
                     GFGPPGGGPGGMMMMMPPPRPGEILPPMVQERLRLSAVQKAELAAIQKEVDERLGNIL
                     TDEQKKQLQEMRQPGPGGFGPRGGGPPGGFGPGGGEPLGQGPRGGFGPPWPR"
     gene            complement(11040..>11505)
                     /locus_tag="VHS97_01505"
     CDS             complement(11040..>11505)
                     /locus_tag="VHS97_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX3446895.1"
                     /translation="DPTGSPINNVTIEIWQCDANQVYLHTGDSANKAGQQDKNFQGFG
                     RFMTSSTGAYYFRTIKPVPYPGRPAPHIHFKVKKGGRELLNSQINIAGHPGNDVDGVV
                     QGGISLFVRELLMTDFTPIKGSKIGELAATFDIVLGRTPDEREFSRGVSSSP"
BASE COUNT         2136 a         3606 c         3499 g         2264 t
ORIGIN      
        1 cgcggccaag aggagccgcc ctcgcggaaa cgcaaacagg ctgtattccg ctcggttcgg
       61 caaggaagca ccgaggagcg cggcgttaag gcccgacgct tatcgaaccc cgaaaggacg
      121 gatccgcgtg accaaattcc aacacgccac gcgcatgggg ttgctcatgg ctgctgccgc
      181 ggtgctgctc ggctgggtgc tccggcacaa agagaccagc tttgccgacg gcctgcgcta
      241 tatccaccag gccgagaaga ttcaggccgc cacatggcgc gatggactgg tcaaaggtat
      301 cgaccacccg ctccacccgc tgggcatcgc cgccgtacat cgcgtgctcg gtggaacgga
      361 ccccacgtcg tggcagattg ccgcatcggt gctctgtttc acaagtgccg tgctgctggt
      421 catcccgatc tatctgctca ccctcgagct gtttggcgaa aagaccgcct ggcttggctg
      481 tctgctggtg atggtcaatc cgatcattgg ttatgtggtg gtcaacgtcc tctccgagag
      541 cacgtttttg ctttggtgga gctttggtct atggggatcg gtccggtttc tccgcgaggg
      601 ccgtttcttc tggctgccgc tggcgatcgg gtttggagca ttggcttacc tgacgcgtcc
      661 cgaagggatg ctgttgcccg cggcgctggt gctcactttg ttggtcttgc cgctgtcgcg
      721 ggcgacgcgc atcaactggc cgcgctggtg gcgtgcgatc gggtttctgc tcaccggcgt
      781 cgtcctgctg gtcggaccgt atattgctct caaaggcagc gtgggaacca agcccgggat
      841 cgcacgtgtg ctgggcttgg ccccgcggtc tttatcgctg gcgctcgagc gggaaaagcc
      901 gattcctccc gaacagaccg acttcgatac gtaccggatc gccacaatcc gcatgctcaa
      961 agtatttcga gcagcagtca cgccgccgct tttcaccgtt gcgttgctcg gaattgtcct
     1021 ggcagcgccc ttggcagcca aaggtcgagc ccgcgccggc ttgttcctga cgatcgtgct
     1081 ggccgcttct gcggttgcgc ttgtacggct tcatgccact ggtggttact gcaccgtgcg
     1141 tcacggtctc gtgcccggca tcatcctcac actggcagcg gcctatggct tgacctggtt
     1201 aattgaaaag gtgtcaatcc cggggcgctg gcttggccag gccgacttac gactgcggcc
     1261 tggccctgcg atctgggcgt tactcattgg gggcgcggtc gttttcccca atattcaatc
     1321 ccagggaccg ccagtacccg gcccgttttc ggtttaccgt gagaccggcc actggatcgc
     1381 tgagaacacc accgcccaag ataaagtgct ggacctgacc gactggtcgc tcttctttag
     1441 cggtcggccc ggctatatct tcgcgaacgt gtacgaggcg cccgccgacc ccagcacgcg
     1501 atggcttgtg gtccgcgcgc cccacgtcaa tggccactgg cattacagcg aagtgcttcg
     1561 cgaactgatc gcgggccgtg aacccgtcgc gctggttccc cccaaggcag atctcaacca
     1621 ggtgcaaatc cgcatctacg accgccaggc cccccctcca ccccagaccg caaccgcaac
     1681 gagctcacgc gacagcacaa cccgacaacg ctaaacggcc ccggacgccg ttcggctgtt
     1741 ccgccgtttg gcgttcggcg cgggtctccg accccactga aacgcgcgac caaaggtctc
     1801 ccgctatagc cgcagccctg gccaccgatc ggcgcggttt tccgacccgc ggcgaagggg
     1861 gttatgccgc gattggaggc cgtcggtcgc acctcgcaag gttttcgtga acatcgtgcc
     1921 cgcttgtagg tgaggggaca gacgggtagg ctcgttggtg gtgtgaacgc aatgcgatag
     1981 ttccaagcgc ggcgagtgcg aggcaactgg gaacgccaat cgatggccga tgccaccgga
     2041 ggggaaaacg gtaaccatag ccggccgcct gacaatgccg gtcgtggacc tcggcagcgc
     2101 cggcagtcaa ggacagggcg tcggccgaag ggagcgctgc gaccggtcgg cccgagcagc
     2161 ggccggggac cgatccgcgt ccgccgaatc ggcggtgagg acttcgagtt catccatccg
     2221 cggggcgtgg aggaggccga gctcgactat gaagagggga tcgagctttg gaaggccggc
     2281 gatccagagt ccgctcggga tgccatacgc tatgcgcttt cggcttatca cgacaacatc
     2341 tggatccacg tggcgctggg ccaaatcgcc cttgtcgagt ttcacgatcc ggcactggcg
     2401 cgtggtcatt tcggctacgc gttcgaactg gcaaaccggg ccttgcccca gggtttcaag
     2461 gggcgtttgc cgcgagagcg gcccaacaat cgaccttttt atgaagccat tgacggtctg
     2521 atccagagct tggaagcgct ggggcggcgt gccgacgtcg acagccttaa agcgcttgct
     2581 tctcggctag cgagcggttc gccttgatgc ctttttggtc acattggacc gcttgttcgt
     2641 aagcgtaaac gcatcgcgcg tcgtcggcac tctggttttt tcgactttag gaatttttcg
     2701 gacttgcagt gattcctcga tcgagataga acgaccgcag aacgagactg acgagccgaa
     2761 ggaatggcct ggcatacacg tccgccgcaa ccaatgccga cggaaaggaa gccgaacgtc
     2821 gatgagcgtt gttgtggttg gcggggtgcc caagtcaact ctggatcgtc gagcccaagc
     2881 gccctggcgc cggcggccgg cgagattggt tcggtgcggg gtctcatgat cgttcgtccg
     2941 atcgccggcg gcgtggaggt cctcgctccg gcgaagttaa acctgttttt ggaggtcttg
     3001 gggcgcaggc ccgacggcta tcacgagatc gaaacgttga tggtcgcagt gagcctgtac
     3061 gacaccttga ccttcaccga cgacccgtcg ggcgcgatca gcctacggtg tagcgatccg
     3121 gcattgccgg tgggaagtga caatctggtc gtgctggcag ccgagcgtct gcgggcgtcg
     3181 gccggctgcc atcgaggggc gcggatcgtg ctcgaaaaga cgatcccgac agaagccggg
     3241 ctggctggtg gttcgagcga cgcggcggca acgctcgcgg ccttggaccg gctctgggat
     3301 ctccaaacgc cacccgccaa gctctctgcc ttggctgggg agatcggcag cgacgtggcc
     3361 ttcttcgcgc atgctcccgc cgcggtttgt cgcggacggg gtgagcgagt cgaaccggtc
     3421 gccctgaagg aagagtatca ctttgtgctc gtttgtccgc cggtcggcat gagcacggtc
     3481 aacgtctacc gccaggtggt tccacccgaa cggccccagc cgatcgggcc ggttttggaa
     3541 gttctggcac acggcggacc ggctgatctg ggtcggagcc tttttaaccg gcttcagttt
     3601 ttcgccgaga cgctcttgcc cgaattgact cgggttaaag acgccctggc gagcctgggc
     3661 tcgttgctcg acgggtccat gatgagtggc agcggctcag cctattttgg gctgtgtcgc
     3721 tctagagccg cggcctgcca ggccgcggac atcctcgaac cgctcggact aggatgggtc
     3781 cgggtcgtga cctgcggccc ctgagcagaa aacgccccgt aaaggagtac cggctgtgga
     3841 gatcactgaa gtccgcatca agctcatgga agacaactcg ggcagcaacg aacggttgca
     3901 agccttttgt agtattacgt tcgacgacat gttcgtcatc cgcgacctga agatcatcga
     3961 aggcgccaag gggttcttcg tggcgatgcc gtcacgcaag ctgacggatc gctgccacaa
     4021 ttgtggcacg aagaaccacc tgcgaagtcg cttctgcaat caatgcggat gccgactcga
     4081 cgaaaaccgc gcgttgcgcg atgccgacgg gcgagccaag ctgcacgccg acatcgctca
     4141 tcccatcaac tctatgtgcc gtgagaagat ccaggccgct gtgctggcat cctacgccga
     4201 ggagctcgag cgctccaaga tgcccggcta catttcgcgc tacgacgaat acgaagtaga
     4261 cgacttcgac gttcaatacg aaggtcatcc caacggtccg cccatgacgg ccgggaacga
     4321 gcctccggtt cggcgggcac ctctgcgtgg ccacacccag cacgcccgcg gtaaccagtc
     4381 cgtcctgcgt gggccccacg tctccgctcg caaggagagc actcccgaag gagtcagctc
     4441 cgatcatcac catcatcatc atcggggcga ctcttcctcg ttcggcaatg gtctttgagt
     4501 gtgtgatcct taacccggtc agtcctcgcg gttttgtctt acttgatcgg aattgcgcta
     4561 cacccggagt cataacttcc cgaggcctag actgatcgaa gaggcatccg acccggcgca
     4621 gcggggctaa agcccgcagc gccgggtcgg ttctatgcgc cctcggtata atgaacgagc
     4681 cgaataagcg atgttgcggc cacagcgtcg acaccgcgtc agcatcgcgg atgacagagc
     4741 aggtcaggcc ttgaacgacg acctttcgac atctcatcgg caacctcgtg acgaccgcca
     4801 ggcgactacc gcgacggcgt ctcgttcggt ctggtcgcgg ctcaaggtct ggctcgcgcg
     4861 gatctttacc ctcgccgctc tgatttgctt gggatttctc accgtgcttt acttcctcca
     4921 agcccgcatg atctttccgg gcgcaagtac ccagggacag agagaagcgc aggtgggatc
     4981 tcgggccgat acagaactcg tggatttgag gacaaaggcg ggcgagcgag tggccgctct
     5041 gtttgcaccg gccctgacgt ccgacggccg gccggatcca cgcgcctccg ttcgcccgac
     5101 gattatctat ttctatggca acgcgatgtg tctcaattac gcgacgcccg agctcgatcg
     5161 cttccgccgc atgggattaa acgtgctcat ccccgattac gtcggctatg gcatgagcgg
     5221 aggcactccg tctgagcggg gatgccaggc gacggcagac acggcctatg actatctggt
     5281 ttcgcggcga ggagtcgatc cccgacaaat tatcgcggcc ggctggtctc tgggaggagc
     5341 cgtcgccatt gatctggcat cgcggcgcac tgtcggaggg ctaatcgcct tcagcacgtt
     5401 cacaagcaca aacgacatgg cccggaccat cgttccgctc aagttgcctc gatggttttt
     5461 cgttcatcgg ttcgaaagtc tgcggaagat acccacgatc gcgtgcccga ttctcctggg
     5521 ccacgggcgt ctcgactcat tggttccttt cgccatgttc gagcgactgg ccgccgcggc
     5581 caagacacct gtaacggcgc tcgtcatcga gcaagctgat cacaatgact tctatgacga
     5641 aggcggccag cgaatcgata acgcgatcgc caaattcgtg tcggagcact tccgcggcga
     5701 gccctgattg gtcgatggac gtcaatgaca ccatcgacag tcaagcgtcc cgagggattc
     5761 cccggagctc tcgtttcagt tcggtagcca tccgccggaa tacgccgtcc caatcgccca
     5821 gggttgtctg gtgaaagagc ctcgcggtgg gataccacag gctgtcgtcg cggtcggccg
     5881 gccagcgcca ctcacccacg gtgctcaggc cgacccaaac gcggcgcccc aatccgcccg
     5941 ccaggtgcgc caccgccgta tcgggtgtga tcaccaggtc gagatgcatc atgatggccg
     6001 ctgtctccat gaagtctcgt cgccggcgcc cgggcagttc gacgacgcgc accgacctgc
     6061 cgtccgactc gagctgatcc aagccgtggt tcgtctgcaa gctgatcaga cggacgcccg
     6121 acagctcggc caccggtgca agatgggaca gccgaaacga ccgccagcga tcgtcgctgt
     6181 ggccggggtt cccttgccac gcgatcccga tcaggaaagg ctttgttccc ccggccctat
     6241 ccgactggtc cagagcatct gcacactcgc cgccttcaag tccaatcgcc ctggaaatct
     6301 cggaacgcca gtggtcgacg agtatcggat cggtggcgag atacggaatc tgggccggca
     6361 gggtgtcgag ggtcgtgccg aaaatggccg gcaggctcag taatggagcc tgaaagtggc
     6421 atttcggctt gtagcaagaa tcgtcgaacg ccaggtcgac tccgtcgcaa cgggcgacca
     6481 gttgcaaaag tggcgtctga caaataacca ggacgtgccc ccctcgacgc ttgaccaagg
     6541 gcgcgaagcg tatgaattgc aaggtatcgc caaagccctg ctcgacatgg agcaggatgg
     6601 tgcgaccatc aagatcgtct ccgttccaca aagtttgatt gatttgataa ccgggatgcg
     6661 ggtcgcactt gagacgccac tcgtactcgc gccagccacg cttgtaatca ccgcatgtaa
     6721 gcaacgcata tgcgagattt cggtgcatct cggggtggtc gggccgatgc cgtaaggctt
     6781 cctcgtaatg atgaattgcc tcaggcgact ttcccatgcg acagaggttc attccgaggt
     6841 tttgcaacgc ttcggccgag tcgggccttt gctcgctcgc ccgcgtgagc caatcgtcag
     6901 cctcatcgaa cttgccctga tcggagagca caatccccag gttcacatgg gcgtcgaagg
     6961 cggcgaggtc gatctggatt gcccgccggt agtagtcgcc ggcctcatcg acgcggccct
     7021 gctggtagag ggccaggccc agattggtta aaattctgta gtcgttgggc tgaagctggt
     7081 cggcctggcg gtaaatcgcc tcggcctcca cgtatcggcg ctgccaccag agtgcggcac
     7141 ccagctcgtt gagagtgtcg acgtcgtcgg gccggagccg gagtgcctcg cgcgagtagg
     7201 cctcggattc ggcataaagg ccttgccgtg acagaaggat cgcctgatcg cgataactcg
     7261 ttgccaagcc cgaatgaacg ctctcggtgt aagtccgggt cggcgcgtcc tctgccaaaa
     7321 cagcgcccgg tatcccttcc atggcggacc ctcctgtgcc gtcgtgcggc ccagattaaa
     7381 tcgtggaagc cgctctcttc caagcccaat tccggcgtgc cactcgcggt gcagcttttc
     7441 tgggccactg agtgaatcca atcacgcaga gccggccaaa acttcggcca tgggaagctc
     7501 gatgttcacg accagcccgg gctgagcatt gcgggcggag atccggcctc ggtgcaggtc
     7561 gacggcgcgg cgggcgatgg ccagtcccag cccgataccg ccactagccc tgctgcgatc
     7621 ccgttcgact cgaaagaacg gctcgaagat cgccgcgagc aactcatcgg gcacgccggg
     7681 gccatgatcg cgcacgctga tcctcgccgc gtcgccagct gagtccaacc cgatctcgac
     7741 cgtggtcccc ttcggtgcgt ggcggacggc gttacgcacg acgttttcga aggccctgcg
     7801 caacaattcg ggttcgccca tgatctcaag aggcggcatg gattctcgtt cgatgcggca
     7861 gcccttggcc caggcctcca cttcacaatc ctcgaataga ccctccagca agaggtcaag
     7921 ctggacgggc tcgaggtggt gggccgttgg atccccttcg gcacgggtca gttgtaggag
     7981 ctcgtttacg agaatcgata gccgaccgat gtcacgcttg atccgaccca agaactctcc
     8041 gggatcgtcg ctggtactgg ccagatcgac ggcgacgtcg agccgtgtca ggggcgatcg
     8101 gagctcgtgc gagacgtcct gtagcagccg gcgctcggcc gatagcaggg tttcgatccg
     8161 cccggccatc tcgtcgaacg agcgcgagag ttcgccgatc tcgtctttgc gggtcgatag
     8221 agcccgggtc gacaggtcac cccggccaaa ctggtccaca aggcgtctga gccgtcggag
     8281 cggggcggcc aggtgtgcgg ccaggatcca tcccatgagc gcgatcacca aaacgatcga
     8341 gccgtaatac ggcaagatgt tgggccgatc gaaccagggt tttacaatcg agatgaaacg
     8401 ataacggttg tcgtgcggcg gacccacgaa gatcatgcgg ccgtcggcaa gccgaggcgg
     8461 acccggagga cgatggcccc gcctgagcag gtcggatcga tcggcgccgc tggccaggtc
     8521 gcgaccacat tcgtcagtca aaagatgctc accaggcaaa taatagtcga gccggcgcag
     8581 ttgacgggca agctgatcgg gcccgcccgt ctcgtaagca tggcgaagat catcctcgac
     8641 catccgcagg aagtgaggaa acgggtctcc cttggatgga ccatgtcgat ccagtgtgcg
     8701 cgagattgcc cagtaagcga ccagggagag gacgaaagtt ccgagcgacc agagcatgac
     8761 cttggagaag atggatcgca cgtcaggttt ctcccggtgc atcaggcccg acccggcata
     8821 gatagccgac gccgcggacc gtccggatcg agcctccgtg ataccccagc ttcttccgca
     8881 ggttgcagat gtgcatgtcg agggtgcggt caaacttggt cgcccgccgc tgatagaggg
     8941 cggccgtcag ctcatcgcgc gagacaatcc gtccagcgga atgggccagg aattcgagta
     9001 tatcgtactc caccgtggtg agcgcgaccg gccgatcttg aaccaaaacc tcgcgcgccg
     9061 gcggaatgat cctgatccca cccagttgca acatctccga ttccttcggt ccccgcacgg
     9121 cccgccgcag cacggcccga atcctggcca tcagctcctc ggtcccgaac ggcttgggaa
     9181 ggtaatcgtc ggctcccgca tcgagacctg ccacacgatc ggactgggtc gtgcgggccg
     9241 tcagcatgat caccggaacc tggctctggc gacggagcag tcgcagcaac tcaaagccgt
     9301 ccaggcctgg catcatcacg tcgagtaata gtaggtcgtg ccctccagcc aaggcccggg
     9361 ccaggccccg ccggccatcg caggcggtct ccagacgaat attgcggcgc gcgaaaaact
     9421 cctgcaagag ctcgcacagc tccgtatcat cgtcgataag taagaccaac atgcccgatc
     9481 gcgcagcgtc cgctccttgc tccagccgaa atcccaggtt cgtgccgtgc gccatcattg
     9541 cttcctgacc ggcagcgacg gaccgcccgc tgatgccgcg cggtcgccct gtacccactg
     9601 ttaatcggaa atgtgcgtct tttcgggttc cagtctgatc cgctcttcgg agggattttt
     9661 ggactggttt atgtccggca actggtcgtc cgacggtggg cccatgcgcg gtggtaatcg
     9721 atctgacctt gaaccgggtc ctgaaatctc tggattccag ggtccgcgat ttccgaacgg
     9781 tctggggaga tgatcgggat ctcgaccatg aaacatcgtg gcttgtatgg cggtgtccaa
     9841 cgatcgtaga ttgatcgaac cctgcccgct cggatccgtc cgtcttacga agtcggctgc
     9901 cgccagcgat gcctcctctt cggagaggcg cccgtcatgg tcgctgtccg ccgttcggaa
     9961 gatccaccgc gccgccacct cggccgaccc gcccgggccg cccggcggcc ttctctgtaa
    10021 ctctcgttcc acgtacccca tcttcttatg agccacccga aagtagccca tgccaacggc
    10081 catggataca agattggcga cgatcatgag cacgatcatg ggcaacagat tttcggtgat
    10141 cttcctgcgc ccgatgcagt agaggcctgc catcgccaag gatatggtaa gagcgaccgc
    10201 gaccgctccg atcagttctt ccggcgatcg atcgataatc gcatttccca atgtgttcag
    10261 atactcggtc acggcggcct ccccagaaat cacgaggaca tcttacccgc accgctctat
    10321 gatcgtatcg caaccggttc ggccgtacgg caggtcatct gggccagggc gggccgaacc
    10381 cgcctcgagg accttgaccc agaggttctc cgcctccagg cccaaaaccc ccaggtgggc
    10441 cgccgccacg tgggccgaat ccaccagggc caggctggcg catctcctgg agttgcttct
    10501 tctgctcgtc ggtcagaata tttcccagcc tctcgtctac ttctttctga atcgcggcga
    10561 gctcggcttt ctgaacggcg ctaagcctta accgctcttg gaccataggc ggcaggattt
    10621 cgccgggtcg cggcggcggc atcatcatca tcataccgcc gggaccgccg cccggagggc
    10681 cgaagccgaa ggaacggccc cgactttttg attcttcctt tgccgccaag ctcgccagct
    10741 cgttcttcgt cacaattcca tcgttattgg cgtcggaacg tgcgaacagc cggtgcagtc
    10801 gctgatcggt cacctcgctc ttggtgagtt tgccgtcctt gtctttgtca aactccatca
    10861 tcctggtcac cagatcgccg ccggtggcaa attgcgaata cacttcctgg ccagggcccg
    10921 gcggctgcgc cgtcgcgcct gaggccagat taagaatcag taccgacgac attaaaccgg
    10981 ccagcacacg catcggagtt ctccctcaaa taacaattga tttccggtag ggtcatgcgt
    11041 caaggtgacg aacttacgcc tcgagaaaac tcccgctcgt ccggtgtacg acccaggaca
    11101 atatcgaacg tcgcggccag ctcaccgatt tttgagccct ttattggcgt gaagtcggtc
    11161 ataagcagtt cccgaacgaa aagactgatg cccccttgca ccacgccgtc gacatcattt
    11221 cccggatggc cagcgatgtt gatctgggag ttcagcaact cgcggccgcc cttctttacc
    11281 ttgaagtgga tgtgcggagc cggccggccg gggtagggga ctggcttgat ggtgcggaag
    11341 taatacgcgc ccgtcgagct cgtcatgaat cggccgaacc cctggaagtt tttgtcttgc
    11401 tgaccagcct tgttggcact gtcgcccgtg tggagataca cctgattggc gtcgcattgc
    11461 cagatctcga tcgtgacgtt attgatcggg ctgcccgtcg ggtcc
//
LOCUS       DATAQL010000087         5981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_924421, whole genome shotgun sequence.
ACCESSION   DATAQL010000087 DATAQL010000000
VERSION     DATAQL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924421"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VHS97_01510"
     CDS             <1..819
                     /locus_tag="VHS97_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3446896.1"
                     /translation="SPHENRPNMIDADAYNNQFVHWSYGLSSYYGGYGGSGGYGGYEN
                     GYSPFLFDSSLYDRRGPSYNNAYYDYAVYGSGAFEYRSLNSVVAQPAVYEYSHPLTTP
                     VTPPQPAVANPVGTGFDASRDAFKAGNYSKALELADQSIRQLPGDGTLHEFRGLVLFA
                     LHRYEDAAAPLHTALSVGRGWDWRTLVGLYSNVSVYTEQLRALENYCNLNPRSASARF
                     VLAYLYLTQGNADVAADQFKQVSALQPENAVAARLIQQLDKTKMPAASDRVRPE"
     gene            complement(955..5313)
                     /locus_tag="VHS97_01515"
     CDS             complement(955..5313)
                     /locus_tag="VHS97_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3446897.1"
                     /translation="MKSIFKLALLLGLALAIDLRGAELPQKDGASPDARPVLKVLFLG
                     DQGHHRPADRAAQLTPVLAGRGIDVTYTEKLTDLNAQNLARYDALLVYANIDRIGSDQ
                     EKALLDYVRGGGGFVPVHCASYCFRNSPACVALVGAEFQRHGTGEFDTKIVDPSHPIM
                     NGFVPFRTWDETYVHHKHNDKDRHVLQTRAEGKSEEPWTWVRTEGKGRVFYTAYGHDS
                     RTWQNPSFHDLIERGIRWASGKGKVYDSRGRVAAGLPPFSYEDSGADIPNYLPGRRWG
                     TQGEAHRQMQNPLPADLSIKHLVVPPGFEPRLFAAEPEIYKPLCMAWDHRGRLWIAES
                     VDYPNTKRRDGRGRDRIAILEDTNGDGRADSFKVFSEGLNIPTSLLCYDGGVIVLQAP
                     DTLFLKDTDGDDRADVNKKLFTGWGIGDTHAGPSNLRWGLDNWVWGIVGYSAFRGTVG
                     GEAIAFGQGFYRFKPDGSKLEFVRSTSNNSWGVGLSEEGIVFGSTANGCPSVYMPIAN
                     RYYEAVRGRAPFKLESIAASNAFYPVTEKVRQVDFHGGFTAAAGHALYTARTYPRQYW
                     NQVAFVTEPTGHLIATFTLQRKGSDIVDYYGWNLLASDDEWTSPITAEVGPDGHVWVI
                     DWYNYIVQHNPTPQGFKTGSGNAYETPLRDKTHGRIYRIVYKDASPSKPVSLDPADAA
                     GLVVALRHDNQLWRLHAQRLLVERKKTDVVPALIELVRDAAVDAIGLNPGAIHALWTL
                     HGLGAISDAGSPAAQAVLAALKHPSAGVRRNAAQVLPADPRSASALLAAGLLSDPDPQ
                     VRLAALLSLTDLPPADQVATALAVALRGGLAKGDQWLADAATAAAAKNSDAFLKALAK
                     KDQKPAGTEVLAIADHVAEHWARGGPSESAGALLAALSAGEPAVDEAILRGAVRGWPK
                     GRPATFDGSMSDTLKRLTVELNPAARAHLVRLVGLWGNQALDRLGAEIAASLLASTQD
                     SKVPEARRIEAARQLIELRPADPAAARALLELIATPVSAELTAGLVDAVGNSKAPEVG
                     KAFVDVLPKLSPAVRSRVLRALLGRSDWIPAFIVDLERDPSRLSELALDQKQALTAHP
                     NRDIAARAQRLLAQGGGLPDPDRQLVIDRLAPELKEGGDPARGKLVFQQQCTKCHRHN
                     GEGGQVGPDLSGMAAIPRHELLIHILDPSRSVEGNFVQYSVATTDGRVIGGLLASESK
                     TSVELIDAEGKRHVVLRDDIDQMAASKKSLMPEGFEKQVPTAAINDLLAFLTQRGKYR
                     PLDLTKAATIVSTRGMFEDTASEAERLVFPDWSTKLVEGVPFMLVDPKGDHVPNVVLL
                     HGPLGKFPPEMPKSVDIPCNTSARAIHFLSGVSGWGFPYGRKGSVSLIVRLHYADGTV
                     ENHPLQNGVHFADYIQVVDVPGSKLAFNLHGQQIRYLSVEPKKKDSIERIELVKGPDR
                     SAPLVMAVTIEVAGAE"
     gene            complement(5433..>5981)
                     /locus_tag="VHS97_01520"
     CDS             complement(5433..>5981)
                     /locus_tag="VHS97_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446898.1"
                     /translation="NLSTGEYLGVRLADLGFTGKEDFEISATLPDIPSLKEVGQFGLY
                     AGLRSDTNIRGGLISRSGPGGPATSDFFRLFLVKNNKGVDFDIYEIGLMTTGDNLRLT
                     LRRLEGRFSLVVDNLTRNSSSTLEISQPDFLDPVKDMHVGLFGANTQSEVPKTLTIRD
                     LKVTVWTTQADNPTIVQSGVGR"
BASE COUNT         1114 a         1829 c         1869 g         1169 t
ORIGIN      
        1 tctccccatg agaatcggcc caacatgatc gacgccgacg cttacaacaa ccagttcgtg
       61 cactggtctt acggcctatc cagttactac ggcggctatg gtggttcggg cggatatggc
      121 ggatacgaaa atgggtactc gccatttctt ttcgactcgt cactctatga ccgacgcggc
      181 ccgagctata acaacgctta ttatgattac gccgtttatg gttcgggtgc gtttgaatac
      241 cgaagcttga actcggtggt ggcgcagccg gcggtttatg agtattcgca cccgctcacc
      301 acgcccgtga cgccgccgca accagcggtc gccaatccgg tgggcacggg cttcgatgcc
      361 tctcgtgatg cgttcaaggc cggcaattat tccaaggccc tcgagctggc cgaccaatcg
      421 atccgccagc tacccggaga cggaactctg cacgagttcc gcggcctagt cttgttcgct
      481 ctccatcgct atgaagatgc ggccgcgcca ctccataccg cgctttcggt cgggcggggc
      541 tgggactggc ggaccttggt aggactttat tccaacgtgt cggtctacac cgagcagctt
      601 cgtgctctgg agaattattg caacctgaat ccgcgctccg cctcggcacg gtttgtattg
      661 gcctacttgt acctgaccca agggaatgcc gacgtggccg ccgatcagtt caaacaggtc
      721 agtgcgcttc agcccgaaaa cgccgtcgcg gcgcgtctga tccagcagct cgataagacc
      781 aagatgccag ccgcgtccga ccgcgtccga cccgaatgac gacgggccga ttctcaccaa
      841 gacaccttga gtgcgatgcc aacaatttgt tgctcagtcg acggtatcga gctcgagttc
      901 aggccgacgc gtgcgttctg tttctgatca gcaccgtcag ccgtttcctc cgggtcattc
      961 cgcgcccgcg acctcgatcg tcaccgccat aaccagtggc gccgaccgat ccggcccctt
     1021 gaccagctcg atacgctcga tcgagtcttt cttcttgggc tcgacgctca aatagcggat
     1081 ttgctggcca tgaaggttga aggcaagctt cgagccgggg acgtcgacga cttgaatgta
     1141 gtcggcgaag tggactccat tttgcagggg gtggttttcg acagtcccgt cggcgtagtg
     1201 caagcgcacg atcaatgaca cgctcccctt gcggccatag ggaaagcccc atccgctgac
     1261 accgctcaag aagtggatcg cccgcgcgct ggtgttgcac ggaatgtcga ccgacttggg
     1321 catctccgga ggaaacttgc ccagcggtcc gtgaagtaag acgacgttgg ggacgtggtc
     1381 tcccttggga tcgaccagca tgaacggcac gccctcgacc agcttggtgg accaatcggg
     1441 gaagaccagt cgctcggcct cggaggcggt gtcctcgaac atgccgcgag tgctcacgat
     1501 cgtcgccgcc ttggtcagat cgagcggccg gtacttgccg cgctgggtga gaaacgccag
     1561 aaggtcgttg atcgcggcgg tgggtacttg cttttcaaaa ccctcgggca tgagcgactt
     1621 cttggacgcc gccatttggt cgatgtcatc gcgcaagacg acatgccgct tcccttcggc
     1681 gtcgatcagc tcgaccgagg ttttggactc ggaggccaaa agcccgccga tcacgcggcc
     1741 gtcggtggtc gcgacggagt actggacgaa gttgccctcg acgctacggc tggggtcgag
     1801 gatgtgaatc aagagttcgt ggcgcggaat ggcagccatg ccggagaggt cggggccgac
     1861 ctggccccct tcgccattgt ggcggtggca cttggtgcat tgctgctgaa aaaccaattt
     1921 accccgcgcc ggatctccgc cttccttgag ctcgggcgcg agccggtcga tgaccagctg
     1981 gcgatcggga tcggggagcc caccgccttg cgccagcagt cgctgggcac gggcggcgat
     2041 gtcgcgattg gggtgggcag tcagggcctg tttctgatcg agtgcgagct cggagagacg
     2101 actcgggtcg cgctcgagat caacgatgaa tgccggaatc cagtcggacc ggcccagaag
     2161 ggcgcggaga actcgtgatc gcacggcggg cgacagtttg ggcaagacat cgacgaacgc
     2221 cttccccacc tcgggcgcct tgctgttgcc gacggcgtcg accaagccgg ctgtcagctc
     2281 ggcggaaacc ggcgtcgcga tcaattcaag cagtgctcgg gccgcagctg gatcggccgg
     2341 ccgcagctcg atgagctggc gtgcggcctc gattcggcgt gcctctggca ccttcgaatc
     2401 ttgggtcgag gcgaggagcg atgcagcgat ttccgcgccc aggcggtcga gggcctgatt
     2461 gccccacagg ccgaccaggc ggaccagatg cgcccgcgcc gccggattga gctcgaccgt
     2521 cagtcgcttg agtgtgtcgc tcatcgagcc gtcaaaagta gccggacgcc ccttgggcca
     2581 gccgcgaacc gcgcccctca agatcgcctc gtccaccgct ggctcgccag cggataacgc
     2641 ggccaaaagc gcaccggccg attcgctggg tccgccgcgg gcccaatgct cagccacgtg
     2701 atcggcgatc gccagcacct cggttccggc gggcttttgg tcctttttag ccagcgcctt
     2761 caaaaacgcg tcgctattct ttgcggccgc cgcagttgct gcgtccgcca gccattggtc
     2821 ccctttggcg aggccgccgc ggagagccac agccagtgca gtcgccactt ggtcggccgg
     2881 cggcagatcg gtcaggctca agagcgcggc cagccgcacc tggggatcgg ggtcgctgag
     2941 caagccggcg gcgaggaggg cgctggcgga tcggggatcg gcgggcaaca cttgagcggc
     3001 gttacggcgc actccggcgg aggggtgttt gagcgcggcc agcacggctt gggccgccgg
     3061 actccccgcg tcggagatgg cccccagtcc atgcagagtc caaagcgcgt ggatggctcc
     3121 cggattgaga ccgatcgcgt cgaccgctgc gtcgcgaacg agctcgatca aagccggaac
     3181 aacatcggtc tttttgcgct cgaccaagag ccgctgggcg tgtagccgcc agagctgatt
     3241 gtcatgccga agggcgacga caaggcccgc ggcatcggcc ggatcgagcg agacgggctt
     3301 ggaaggcgac gcatctttgt agacgatccg atagatacgc ccgtgcgtct tgtcgcgcag
     3361 cggggtttca tacgcgttac cgctgccggt cttgaatccc tgcggcgtcg ggttgtgctg
     3421 gacgatgtag ttgtaccaat cgatcaccca gacgtgtccg tcgggcccga cttcggcggt
     3481 gatcggcgac gtccattcgt cgtcgctcgc gagcaagttc cagccgtagt aatcgacgat
     3541 gtcgctccct ttgcgctgga gtgtgaacgt ggcgatcaaa tggccggtcg gctcggtcac
     3601 gaacgcaact tgattccagt actgacgcgg ataggtccgg gccgtgtaaa gagcatggcc
     3661 ggccgccgcg gtgaacccgc cgtgaaagtc gacctgtcgg accttttccg tcacgggata
     3721 aaacgcgttg gaggcggcga tgctctcaag tttgaaggga gcccgaccgc gaaccgcctc
     3781 gtaatagcgg ttggcgatcg gcatgtatac gctggggcag ccgttggccg tcgagccgaa
     3841 aacgattccc tcttcgctca agcccactcc ccacgagttg ttgctggtac ttcgcacgaa
     3901 ctcaagtttg gatccatcgg gcttgaaccg ataaaacccc tggccaaacg caatagcctc
     3961 gccccccacc gtgccgcgga aagcggaata gccgacgatt ccccagaccc agttatccag
     4021 cccccagcga agattgctcg ggccggcgtg cgtgtcaccg atgccccagc cagtaaagag
     4081 tttcttgttg acatcggctc gatcgtcacc gtcggtgtct ttcaagaaga gcgtatcggg
     4141 cgcctgcagg acgatcacgc cgccgtcata acataagagg ctggtgggga tgttgagacc
     4201 ctcggagaag accttgaacg agtcagcccg gccgtcgcca ttggtatctt ccagaattgc
     4261 gatccgatcg cggccccggc cgtcgcgcct tttggtgttg gggtaatcga cgctctcggc
     4321 gatccagagt ctcccccggt ggtcccacgc catgcagagc ggcttgtaga tttcgggctc
     4381 ggcggcaaag agccgcggct cgaagccagg cggtacgact agatgcttga tggatagatc
     4441 ggctggaagg ggattttgca tctggcggtg cgcttcgcct tgtgtccccc accgccgacc
     4501 agggagataa ttggggatgt ccgccccgga gtcctcgtag gaaaacggcg gcagaccggc
     4561 cgcgacccgg ccgcggctat cgtagacctt ccccttgccc gaggcccaac gaataccgcg
     4621 ctcgatcaag tcgtgaaagc tggggttctg ccaggttcgc gaatcgtggc cataggccgt
     4681 gtaaaagaca cgtcccttgc cctcggtcct tacccaagtc cagggctctt ccgattttcc
     4741 ctcggctcgc gtttgcagaa cgtggcgatc cttgtcgttg tgtttgtggt gcacataagt
     4801 ctcatcccag gtgcggaacg ggacgaagcc gttcatgatc ggatgagacg ggtcgacgat
     4861 cttggtgtcg aactccccgg tgccgtgccg ttggaactca gcgccgacga gcgcaacgca
     4921 tgcgggcgaa ttccggaagc aataggatgc gcagtggacc gggacaaatc cgccaccgcc
     4981 acggacatag tcgagcaggg ctttttcctg atcagagccg atcctgtcga tattggcgta
     5041 gaccaaaagc gcatcgtagc gcgccaggtt ttgtgcgttc aggtcggtga gcttctcggt
     5101 gtaggtgaca tcgatcccac ggccggcgag caccggcgtc aactgcgcgg ctctgtcggc
     5161 cggccggtgg tgaccctggt cacccaggaa caagaccttg agcaccggcc tagcgtcggg
     5221 acttgctcca tccttctgcg gcagttcggc ccctcgcaag tcaatcgcca gagccaatcc
     5281 aagtaagaga gcgagcttga atattgattt catgagagaa agtctcgggg ttgcgcgggg
     5341 agtccgtatg tctggccgct gtcacgtgcc aaccgcacca gaatcgcatt atcgaaaacg
     5401 agagccgaga agttcggagc caatcgaaca attcaccgtc ctaccccgct ttgaactatt
     5461 gtgggattgt cggcttgcgt cgtccagacg gtgactttca ggtcgcggat cgtcagagtc
     5521 ttggggactt cactctgcgt gttggcgccg aagagcccga cgtgcatatc ctttacagga
     5581 tcaaggaagt cgggctgaga aatctccagg gtgctcgaac tgttacgggt caggttgtcg
     5641 acgaccagcg aaaaccggcc ctccagccgc cgcaaggtca aacgcaggtt atctccggtc
     5701 gtcatcaggc cgatctcgta gatgtcgaaa tcgacaccct tattgttctt gacgaggaac
     5761 aagcgaaaga aatcggatgt cgccgggccg cccggcccac tgcggctgat caatcccccc
     5821 cggatattcg tatcgctgcg caatccggcg tataggccga attgaccaac ctccttgagg
     5881 ctcgggatat cgggaagggt cgcgctgatc tcgaagtctt ccttaccggt gaaacccagg
     5941 tcggcaagcc ggactcccag atattcgccg gtcgacagat t
//
LOCUS       DATAQL010000088         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2756951, whole genome shotgun sequence.
ACCESSION   DATAQL010000088 DATAQL010000000
VERSION     DATAQL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2756951"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1713
                     /locus_tag="VHS97_01525"
     CDS             <1..>1713
                     /locus_tag="VHS97_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3446899.1"
                     /translation="PAAPVFRISPAEPWRVVRTRQRAADPVLSRRLPPTELFAAGFFT
                     SATGVTVYRGSSYPPNYRGNVFVGDVGGNLVHRKILDAEGPTYRAWRADRNTEFLAST
                     DNWFRPVNFANTPDGTLLVIDMYRETIEHPHSIPEPIKKHLDLTSGNDRGRLYNLAYA
                     GSARRPRPNLGHAPADELVRLLADPDSWWRETAQRLLFERQDRSVVESLRKMVKARPT
                     ALGRLHALWTLQLLTGLDSESIVRGLEDPEPRVREQAILLAEARLPREPELLAKLLPL
                     AGDKDPMVRLQLAFSLGEANTDPRVIAALASIGTADAPSVWTRTAVSSSIAGRSLAFL
                     GALGIQKGFLTTDAGRAWLDELAFLVGSERDPVQSTRLLDAMNDARIGTQLLMRAAVA
                     LGRARQRAGGSIRPVLEGTKSNLAASLLADAVRFAGSDGQVEDRLVAIKLLRLADQKA
                     ARILFPSLLDARQPMAVQLGVLQALGGLFDRDVARQVVMHWRSMSPTVRREGAEVLFS
                     RRDGVEAVIGAIESRAILPSELDPVRLKELDAYADPSLQTRARKILAADGSTSRNRGQ
                     VVASY"
BASE COUNT          307 a          547 c          546 g          313 t
ORIGIN      
        1 ccggccgcgc cggtgtttcg gatcagcccg gcggagccct ggcgggttgt acgaacccgc
       61 cagcgcgcag ccgatccggt gctctcgcgt cgcctgccgc cgaccgaact tttcgccgcg
      121 ggcttcttca cctcggcgac cggcgtcacg gtttatcgcg ggtcgtccta tccgccaaat
      181 taccgcggca atgtgttcgt gggggacgtg ggcggaaatt tggtccatcg caagattctc
      241 gacgccgaag gaccgacata tcgcgcctgg cgggccgatc ggaacaccga attcctggcc
      301 tcgaccgaca actggtttcg gccggtcaac ttcgcgaata cgcccgatgg gacactgctc
      361 gtgatcgaca tgtatcgcga aacgatcgag catccgcatt cgatcccgga accgatcaaa
      421 aagcacctgg acttgaccag cggcaacgac cgtggccgac tttataacct ggcttatgcc
      481 ggctccgcac gtcggccccg gccgaacctc ggccatgcgc cggcggacga actggttcgg
      541 ctcctcgccg atcccgactc ctggtggcgc gagacagcgc agcggctact gttcgaacgt
      601 caagatcggt cggtcgtcga aagcttgagg aaaatggtca aggcccgacc gaccgcgctg
      661 ggacgactgc atgcgctctg gacacttcaa ctacttacgg ggctcgatag tgaatcgatc
      721 gtgcgcggcc tggaggatcc ggagccgagg gttcgcgaac aagccattct attggccgag
      781 gcccggctgc cacgcgagcc ggaactcctc gcgaaactgc tgccactggc cggcgacaaa
      841 gatccgatgg tccgactgca actggcgttc tcgctgggcg aggccaacac cgatccgcgc
      901 gtgatagcgg ccctggcttc gatcggcacg gccgacgctc caagcgtttg gactcgcacc
      961 gccgtctcga gctcaatcgc ggggcgttcg ctcgcgttcc tgggcgcgct gggcatacaa
     1021 aagggatttc tcacgaccga cgcagggcgg gcatggcttg acgaactggc cttcctggtt
     1081 ggatcggagc gagacccggt gcaatcgacc cggctgctcg acgcgatgaa cgatgccaga
     1141 atcggtactc agcttttgat gcgcgcggca gtggcactgg gtcgcgctcg acaacgcgca
     1201 ggaggatcaa ttcgaccggt gctggaggga acgaaatcaa atctggcggc atcactcctg
     1261 gccgacgcgg tccgattcgc cggctcggac ggtcaggtgg aagatcgtct ggtggcgatc
     1321 aagctcctga gattggcgga tcaaaaggcg gctcgcattc tgtttcccag ccttttggac
     1381 gcacgccagc cgatggctgt tcaactggga gtcttgcagg ctctcggcgg gcttttcgac
     1441 cgcgatgtcg cccgtcaggt cgtcatgcac tggaggtcga tgagccccac agtgcggcgg
     1501 gagggcgccg aggttctctt cagtcgacgc gacggcgttg aggctgtaat cggagccatc
     1561 gagtcgcgag caatcctccc ttccgagctc gacccggtcc ggctgaaaga gcttgatgcc
     1621 tacgcggacc catcgctcca gacacgggcc cgcaagatcc ttgccgctga cggatcgacg
     1681 tcgcgcaacc gcggtcaggt cgtcgcttcg tac
//
LOCUS       DATAQL010000089         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1688511, whole genome shotgun sequence.
ACCESSION   DATAQL010000089 DATAQL010000000
VERSION     DATAQL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688511"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="VHS97_01530"
     CDS             complement(<1..1672)
                     /locus_tag="VHS97_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX3446900.1"
                     /translation="MFKDVKRRHCFSGACIGLLAVVFGSSTGFAQSPVQYCNATPTPP
                     FCSAVPGDRSEGWAQQKRSEVMAQHGIVTTSQPLAAQAGLEILKQGGNAIDAAVATAA
                     MLNLVEPMNVGMGGDLFAIVYIAAENKIHVLNASGKAPTGATLARYNSLGYSWNPANF
                     GPGSGMPGGGILSVTVPGAAWGWDEVVRKYGRLSLKEDLEQAAEYAEDGFPVSERISN
                     DWHLPNALPLVHCCTALDQDSVNTWYVNGKGPAPGQIFKNPDLARSFRLLQKHGRDAF
                     YKGEIASAIVAKSTALGGTMTMADLANYSGEWVTPAQTSYHGFQVAELPPPSQAWNVA
                     EMLNILEACVPKWVPGQTLASLGPANPEFWHLIVEAKKLSYADLYAYNGDPDFVNVPL
                     GTLLSKSYAASLCGKVDPHHASGTSAGGNVGSIGDTIVLSTADRWGNMVSWVNSNFAG
                     FGSGITVPGYGFILHNRGSLFTLNPNSPNVIMPQKRPYNTLSAGFAVNDDVRMAFQLM
                     GGDMQSQGHMQVLVNMIDLGANLQAATDMARFFHSQVSNTLSLETELYN"
BASE COUNT          379 a          586 c          653 g          386 t
ORIGIN      
        1 gattatacaa ctccgtttcg agactcagcg tgttcgagac ctggctgtgg aagaaacggg
       61 ccatgtcggt cgccgcctgc aagttggcgc cgaggtcgat catgttgacc agcacttgca
      121 tgtggccttg cgactgcatg tcgccgccca tgagctggaa ggccatccta acatcgtcgt
      181 tcacagcgaa gccggcggag agcgtgttgt agggacgctt ctgcggcatg atcacattgg
      241 ggctgttcgg gttcagcgtg aacagcgagc cgcggttgtg caggataaag ccgtagccgg
      301 gcacggtgat gccggagccg aagccggcga agttgctgtt gacccaggag accatgttgc
      361 cccagcggtc ggcggttgac aggacgatgg tgtcgccgat gctgcccacg ttgccacccg
      421 cactggtgcc cgacgcatgg tgcggatcga ccttgccgca gagcgacgcg gcgtaggatt
      481 tcgacagcag agtgccgagc gggacgttca cgaagtccgg atcgccgtta taggcgtaaa
      541 ggtcggcgta ggacagcttc ttggcctcga caatcaggtg ccagaactcg ggattggccg
      601 ggccaagact ggcgagcgtc tgaccaggga cccacttcgg cacgcaagcc tcaaggatat
      661 tgagcatctc agcgacgttc caggcctgcg atggaggggg cagttcggcg acctggaagc
      721 cgtggtagct ggtctgcgcc ggcgtaaccc attcgccgct gtaattggcg agatcggcca
      781 tggtcatggt gccgccgagc gccgtcgact tcgcgacgat ggcgcttgcg atttcgccct
      841 tgtagaaggc gtctcggccg tgcttctgga gcagccggaa cgagcgcgcg agatcggggt
      901 tcttgaagat ctgcccggga gccggaccct tgccgttgac ataccaagta ttgacggaat
      961 cctgatcgag cgcggtgcaa cagtggacga gcggcagcgc gtttggcaga tgccagtcgt
     1021 tcgagatacg ctcagagaca gggaagccgt cctcggcgta ctcggccgcc tgctcgaggt
     1081 cctccttgag gctgaggcgg ccatatttcc gcaccacttc gtcccagccc caggcggcgc
     1141 cgggcaccgt gacactgagg atgccaccgc cgggcatgcc cgagccgggg ccgaaattgg
     1201 cggggttcca agagtaaccc agcgagttgt agcgagcgag cgtcgccccg gtgggcgcct
     1261 tgccgctggc gttcagcaca tggatcttgt tctctgcggc gatgtagaca atagcgaaca
     1321 ggtcgccgcc catgccgaca ttcatgggtt cgaccaggtt gagcatggcc gccgtggcga
     1381 ccgcagcgtc gatggcattg ccgccctgct tcaggatttc gagcccggcc tgggccgcca
     1441 gcggctggct cgtggtgacg attccgtgct gcgccatcac ctccgatcgt ttctgctgcg
     1501 cccatccttc cgaccgatcg ccggggacgg cgctgcagaa gggaggcgtc ggcgtggcgt
     1561 tgcagtattg caccggcgac tgggcgaagc cggtgcttga gccgaagacg actgcgagga
     1621 ggccgataca ggccccgctg aaacaatgtc tgcgctttac gtccttaaac atcgagacac
     1681 tccctagagg ttatatttcg ttgagccatc ttcagtggct gtttttcaga gactttttct
     1741 cgaaatccac gacagcaacg atccggacga ccgatgccct ggtcaaaccg agacatcgcg
     1801 cggcctccgt tgccaatcga tccggcgaag ttgacagtcg cattgtcgtt gagtcaacac
     1861 ttcaaattgt aaacatggcc tggacaactg gagcgtcgga gcacatcgcc gaatgttgcg
     1921 gccgatttac acggacatga cgcttgggca gcgtgacgcc aagcgatcgc cgaaatgttt
     1981 tcgagcgaag cggaaacgac tcac
//
LOCUS       DATAQL010000090         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3366830, whole genome shotgun sequence.
ACCESSION   DATAQL010000090 DATAQL010000000
VERSION     DATAQL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3366830"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..888
                     /locus_tag="VHS97_01535"
     CDS             70..888
                     /locus_tag="VHS97_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446901.1"
                     /translation="MSRRTRRQTTSGSTLKIYLQEIKNTPLLSAAEEQELAGRVAAGN
                     PYARDHMIKANLRLVVNIARGYVGKGLCLEDLIEEGNLGLIRAVEGFDGAMDTRFSTY
                     ASFWIKQSIRGAVMKNGKPIRLPGYMVKLLSKWQRANYLLRERLGRTPTPDEVGKSLK
                     LSKKRIGMVAKAIQVNELMSHPDEMAGYDFRLDNMLIDERAKAPEAILIESDDVEWVL
                     DRLDDRGATVIRMRFGLEPYSPMTLHEVGEKLGLTRERVRQLEKEAMERLMEAV"
     gene            complement(1388..1570)
                     /locus_tag="VHS97_01540"
     CDS             complement(1388..1570)
                     /locus_tag="VHS97_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446902.1"
                     /translation="MRSTTSSPAATAARTSLRQTEATDSELAEELGVSRLDSVPNLTR
                     RFVKWLADREAMRKRL"
     gene            complement(1973..3073)
                     /locus_tag="VHS97_01545"
     CDS             complement(1973..3073)
                     /locus_tag="VHS97_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3446903.1"
                     /translation="MPTVADVLRRYGPAYLERFGSTMPREHKKVLAAITACRTGQLGG
                     VVYQCESCGATHTMGRSCGNRHCPGCQKDKTKAWLENQLERLLPCPYFLITFTVPAGL
                     REFVRRHQRIAYAAMFEASSAAIKTLAADPKFVGTARCGFFGVLHTWGRTLEYHPHVH
                     YVVPGGGLSDDGTEWLPSRADFFVPVRALSILFRAKFRDALDRAGLLIEVDGAVWRQD
                     WVVHSQAVGDGRTSLQYLAPYVFRVAISDRRIVSCEDGRVTFSYRKSGSRRWRTMTVD
                     ATEFIRRFLQHVLPSGFPKVRHYGFLSGSSGASIAAIRWLIALFYGLAHRLHADPIRA
                     PADAVPIRCPVCSGPMRVLTFLSREAAFFDTS"
     gene            complement(3078..3944)
                     /locus_tag="VHS97_01550"
     CDS             complement(3078..3944)
                     /locus_tag="VHS97_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX3446904.1"
                     /translation="MSSLLRQRMHEDLQLAGLADGTQTLYLRVVKQLAAHYRKAPDQL
                     TEQQVRDYLLYLKNERRCSPSTLKIAASGIIFFYSHTAPRNWQTLKKLRFPRPQALPD
                     VLSIDEVRRLIDAVRTSHNKAFFWTVYSLGLRLQEGLNLHVGDIDSARMVVHVHRGKG
                     AKDRYVPLPPRTLTVLRQYWATHRHPVFLFPATGRDHAQAHTATEPMARPSVHGAMKR
                     VVQELGFRKRVTIHTLRHSYATHLLEAGVNLRLIQQYLGHGSLQATMIYLHLTSLGQD
                     QARATINKLMSE"
BASE COUNT          829 a         1265 c         1434 g          738 t
ORIGIN      
        1 aagcacgcta cgcccacccc ccaggcccaa tcccaacgac gaacaccacg cgttcgaagg
       61 agctcgacga tgagccgccg cacccggcgg cagaccacca gcggttcgac cctcaagatc
      121 tatctgcaag agatcaaaaa caccccgcta ctgtcggcgg cggaggagca ggagcttgcc
      181 ggccgggtag cggcgggcaa cccctacgcc cgcgaccaca tgatcaaggc gaacctgcgg
      241 ctcgtggtca atatcgcgcg gggttatgtg ggcaaggggc tctgcctcga ggacctcatc
      301 gaggagggga accttggcct gatccgcgcc gtcgagggct tcgatggcgc catggacacc
      361 cgcttcagca cctacgccag cttctggatc aagcagtcga tccgcggcgc cgtgatgaag
      421 aacggcaaac cgatccgact gcccggctac atggtcaagc tcctgtcgaa gtggcagcgt
      481 gcgaattacc ttctgcggga gcgcctgggg cggactccga cgcccgacga ggtgggcaag
      541 tcgctgaagc tctcgaagaa aaggatcggc atggtcgcca aggcgatcca ggtcaacgag
      601 ctcatgtccc atcccgacga gatggccgga tacgactttc gcctcgacaa catgctgatc
      661 gacgagcggg cgaaggcccc cgaggcgatc ctaatcgagt cggacgacgt cgagtgggtc
      721 ctcgaccgcc tcgacgatcg cggggcgacg gtaatccgga tgcggttcgg cctcgagccc
      781 tattccccga tgaccttgca cgaggtcggc gagaagctcg gcctgacccg ggaacgcgtc
      841 cggcagttgg agaaggaagc gatggagaga ttgatggagg cggtgtgagc cgcattcggg
      901 tctccctgga gttactttgg ggtcggacct gggttctggt ttttgctgga ggcagggcct
      961 aacgaacgga caattgcgct tccaatgttc taaattgctt gcgcatgccc tcccgatccg
     1021 ccagccacct cacgacccga cgcgtcaggt tcagaacgct gtccggccgg gacacacccc
     1081 ctaactcggc ccgctcgtcg aagatggcca cggtgtgcca gcccaaagtc gtagcggtcg
     1141 ttgccgcggg ccgtgaggtg gtcgatcgca tcgaggtatt gaattaggag tcgccgtatc
     1201 ataatcgcga ttgagcgtcc gatccgagtc aaagcaagga aaagaaccgg gaatctggtc
     1261 cgaccccaaa ggcccccctg ccgggacctc acgacgttgt gacgtccaag gtactttggg
     1321 gtcggaccag ggttctggtt tctgccggaa gcagggccta acgaacaggc cattgctctt
     1381 ccaactttca cagtcgcttg cgcatggctt cccgatccgc cagccacttc acaaaccgac
     1441 gcgtcaggtt cgggacgctg tccaggcgag acacgcccag ttcctcggcc agctcgctgt
     1501 cggtcgcctc ggtttgccgg agagatgtcc ttgcggccgt tgccgcgggc gatgaggtgg
     1561 tagatcgcac cggcggattg gattcgaagt cgccgtgcca tactcgcgat gagagcatcc
     1621 gatccgaatc aaagcgagga aaaacttata aaggatttga tctcgcgaag ccgagatcaa
     1681 gtccatggtt caagaagccc gggaccgagc gtagcggcct ggtcataggg taatggttcg
     1741 atgtcgcgaa ccgaccatcg gctcagtggc acaagcaccg gccgtgtcgc tggagtgatg
     1801 gaggtcgcga ttcgagaacc agcggtgggc gcagcggcaa tggcaggccg gttgttcgct
     1861 gttgcgacgc gtcggtgacc ttggatggat cttcaatgcg gacgccgaac gagcttcggc
     1921 tgggcgaagg agacgacgac acgcagcaag tgggatgaat cgcgctcatt ggctagctcg
     1981 tgtcgaagaa agccgcttcc cgcgacagga acgtgagcac gcgcattggt ccactgcaga
     2041 ccgggcaacg gatcggaacc gcgtcggctg gcgctcggat ggggtccgca tgcagtcgat
     2101 gggccagccc gtagaacaac gcgatcagcc agcgaatcgc tgcgatcgac gccccgctgc
     2161 tgccactgag gaagccgtag tgccgcacct tcgggaaacc gctcggcaag acgtgttgca
     2221 agaagcggcg aatgaactca gtggcgtcca cagtcatcgt ccgccaccgc ctcgagccgc
     2281 tcttgcgata ggagaacgtc actcggccgt cctcgcacga tacaatcctc cggtcgctga
     2341 tcgcgacgcg aaagacgtag ggggcgaggt actgcaacga ggtgcgtcca tcgccgaccg
     2401 cctgggaatg aaccacccag tcttgccgcc agacggcccc atcgacctcg atgagcaggc
     2461 ccgctcggtc gagcgcatcg cggaatttgg cgcgaaagag gatcgacaat gccctcacgg
     2521 ggacgaagaa gtcggcgcgt gaggggagcc attctgtgcc gtcgtcgctg agaccgccgc
     2581 ccgggacgac gtaatggacg tgagggtggt attcgagcgt gcggccccag gtgtggagca
     2641 cgccaaagaa cccgcaccgt gccgtgccga caaatttggg atcggcggcc agcgtcttga
     2701 tcgcagcgct ggacgcctcg aacatcgcgg cataggcgat ccgctggtgc cggcgcacga
     2761 actcgcggag tcccgccggg accgtgaagg tgatcaggaa gtaggggcac ggcagcaggc
     2821 gctcaagctg gttctcgagc caggccttgg tcttgtcctt ctggcaaccg ggacaatgcc
     2881 ggttgccgca ggagcgtccc atcgtgtgcg tcgctccgca cgactcacac tggtagacca
     2941 cgccccccaa ctggccggtg cggcaggcgg tgatcgccgc gagcaccttc ttgtgctctc
     3001 ggggcatcgt gctgccgaac cgctcgagat aggccgggcc ataccgccgc aacacgtcgg
     3061 cgacggtggg catcggacta ttcggacatc agcttgttga tggtcgcgcg tgcctggtcc
     3121 tggccgagcg atgtcaaatg gagatagatc atcgtggcct gcagcgagcc atgcccgaga
     3181 tactgctgaa tcagccgcag gttgacgccg gcttcgagca gatgggtggc gtagctgtgg
     3241 cgaagggtgt ggatcgtgac tcgcttgcga aagccgagct cctgcacgac ccgcttcatc
     3301 gcgccgtgga cgctggggcg ggccatgggt tccgtcgccg tatgggcttg cgcatggtcg
     3361 cggcccgtgg ccgggaagag gaagaccggg tggcgatgcg tcgcccagta ctggcggagg
     3421 acggtcaagg tgcgaggcgg cagcgggacg tagcggtcct tggctccctt accccgatgg
     3481 acatgaacga ccatccgggc gctgtcgatg tcgccgacat ggaggttcaa gccctcctgg
     3541 agccgcagtc cgagcgagta gacggtccag aagaaggcct tgttgtggga agtgcggacg
     3601 gcgtcgatga gtcggcggac ctcgtcgatg ctcaggacgt cgggcagcgc ctgcggcctg
     3661 gggaagcgga gtttcttgag ggtttgccag ttgcggggag cggtgtggga gtagaagaag
     3721 atgatgccgc tggcagcgat cttcaacgtg ctgggggagc aacggcgctc gttcttgagg
     3781 tagagcaggt agtcgcgcac ctgctgttcg gtaagctgat cgggggcttt gcggtaatgg
     3841 gcggcgagtt gcttgacgac gcggaggtag agcgtctgtg ttccgtcggc gagtccggcg
     3901 agctgaaggt cctcgtgcat gcgctggcga agcagggagc tcatgagcgg tcctttccaa
     3961 acggagggtt gaacaaccgt ctggaaagca ctctgtcccc gcggcgtccg tcggtcagca
     4021 gggcaacctg tgaccgggag aggatcgacg acggaaccgc gaaccgaggc cgcgaatcat
     4081 cctgccgcga agcggcttac ttgaaccatt aacctggtcc gacccgtaag cccttggtcc
     4141 gacccgtaag ccctcgtaag ccctgagtaa aatcgatagt catgaggcgc ccgaagcaaa
     4201 cgaagccggt gcggcgttcc gactgtgcgg ttgcttgcac gttggacatc attggcgatc
     4261 gctgga
//
LOCUS       DATAQL010000091         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466 k141_9232,
            whole genome shotgun sequence.
ACCESSION   DATAQL010000091 DATAQL010000000
VERSION     DATAQL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9232"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..217)
                     /locus_tag="VHS97_01555"
     tRNA            complement(74..217)
                     /locus_tag="VHS97_01555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(121..123),aa:Leu,seq:caa)
     gene            502..1860
                     /locus_tag="VHS97_01560"
     CDS             502..1860
                     /locus_tag="VHS97_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX3446905.1"
                     /translation="MSKTYREWCPDQSYMFPPSPRDWLPENDLVYFLVDTVATLDLTP
                     IFAHYERELRGQPPFHPRMMVALLLYCYATGTRSSRKIMRRCQTDVACRVIVGEDVPD
                     FRTISDFRKTHLARLETLFLEVLKLCALAGLARVGTIALDGTKVKANASRHKAMSYDR
                     MKEEEKRLKEEIARLMAEAEATDAAEDEAHGHDRRGDETPDELKRRASRLAKILEAKA
                     LLEERARAEAAEEAARRQAEGKQPPAKPPTEAVPEPKDQINFTDPQSRIMKVSNKGWD
                     QCGNAQAVANEDQIILAADVTDQANDKRQAVPMVDQTLENLEAAGVEQPVGAAALDSG
                     YYSEANTTALEERGIDPHIATERLKHNEEIPPVPRGRIPQNLSAKQRMARKLRTKKGR
                     ETYAKRKGIIEPIFGQLKHVLGFRQFSLRGLVSMRGEWRLMCTVHNLLKLWRHAQVAT
                     AE"
BASE COUNT          492 a          694 c          742 g          436 t
ORIGIN      
        1 accgacaggt cgggtggcct gagggttctc tacgtctgct agcctcgata acgccgactc
       61 gcgctccatt caataccgaa ggtgggactt gaacccacac cctcttgcga ggaccggatt
      121 ttgagtccgg cgcgtctgcc atagtcgtgg tattggaaac acgttgcagc gatgcttaaa
      181 gtcggcgaat ccaaggctcg cacgtcgcca ccttggtgtt ttcgcattgt ggccgcatgg
      241 ggctgacgtt gccaatactg ttcccatttt gttcccaact ggcgcccaac tggcggcttc
      301 tacaggcttg atcatgcgtg atgggctgac gcgggcagtg gccgatgccg gatcacggct
      361 gcaaaccctg tccccggtga tccgtgggcg atgccggaac ggccttccta ggagtctgtc
      421 cgagtattgc ctttcgaagg cgagattcga ataatccgtt ggctcgtggt ttccagtcac
      481 ctgatccagc gagattgtgg catgagcaag acgtatcgcg agtggtgccc tgaccaatcc
      541 tatatgtttc caccttcgcc gcgggattgg ctgcccgaga acgatctggt ctacttcctc
      601 gtcgataccg tcgccacgct cgacctgacc cccatcttcg ctcattatga gcgcgaattg
      661 cgggggcaac cgccgttcca ccccagaatg atggtcgcgt tgcttctgta ttgctacgcc
      721 accggcactc gttcctcgcg gaaaatcatg cgccgctgtc agaccgacgt cgcctgccgg
      781 gttatcgtcg gcgaggacgt tcctgacttc cgcacgatca gcgacttccg caagacccac
      841 ctggctcggt tggagactct cttcctcgaa gtgctcaagc tttgtgctct ggccgggctg
      901 gctcgggtgg ggaccatcgc cttggacggg accaaggtca aggccaacgc ctcgcggcac
      961 aaggcgatga gctatgaccg gatgaaggag gaagagaagc ggctcaagga ggagatcgcc
     1021 cggctcatgg ccgaggccga ggcgaccgac gcggccgagg atgaggcgca cggtcacgac
     1081 cgacgcggcg acgagactcc cgacgagctg aaacggcgcg cgagccgatt ggccaagatc
     1141 ctggaggcca aggcgttgct ggaggagcgg gcgcgagctg aggcggccga ggaagcagcc
     1201 cgccgccaag ccgagggcaa gcagccgccg gccaaaccac caaccgaggc ggttcccgag
     1261 cccaaggacc agatcaactt caccgatccc cagtcgcgga tcatgaaagt ctcgaacaag
     1321 ggctgggacc aatgcggcaa cgcccaagcg gtggccaatg aggatcagat catcctggcc
     1381 gccgacgtga ccgatcaggc caacgacaag cgccaggcgg tgccgatggt ggaccagact
     1441 ctggagaacc tggaagctgc tggagtggag caacccgtcg gagccgcggc gctcgactcg
     1501 ggttactaca gcgaagccaa cacgacggcc ctggaggaga gaggcatcga ccctcacatc
     1561 gcgaccgagc gattgaagca caatgaggaa atccccccgg taccgcgtgg tcggattccc
     1621 cagaacctgt cggccaagca acggatggcg cggaagctcc gcacgaagaa aggccgcgag
     1681 acatacgcga agcgaaaggg gatcatcgag ccgattttcg gtcagttgaa acatgtactg
     1741 ggattccgcc agttctcgtt gcggggcctg gtgtccatgc gcggcgaatg gcggttgatg
     1801 tgcacggtgc acaacctgtt gaagctgtgg cggcacgccc aagtggcgac ggccgagtaa
     1861 ttgaccctgc ccgatcgccg aaatcgaccg gcgatgagtg aaacaaggtc cgatgaggct
     1921 ctcgggcttt gatgagtgct gatccggccg agcatgagaa agaaatgctc cgacaccagg
     1981 tgtcggaaca ggttcgtcaa agtggcgtta aacagacaga ctcctagcta gaggcacaaa
     2041 accgcgtcga ggagtggccc tcggtcgtgc cagccggggt gtcttgttgg cctcggcttt
     2101 cggcgttggt tcaaatccag tcgccccgac tcttttcaga aagaagcctt tcggcgagaa
     2161 cgtcgaaggg cgttctcatt gtggggacaa gagttgcgcc gtcgagcgtg cagttcaaac
     2221 acacgatttc gaggattcga cgctttgcgc cataatcggc ggtaagccgt tgctgctgcc
     2281 ggagtgtttg cgaagttcaa aaactttcgc cgcccactct gccgtttcgt cgtgggagct
     2341 cacaccgacg agaaaaaagc gagg
//
LOCUS       DATAQL010000092         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3062346, whole genome shotgun sequence.
ACCESSION   DATAQL010000092 DATAQL010000000
VERSION     DATAQL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3062346"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..406)
                     /locus_tag="VHS97_01565"
     CDS             complement(59..406)
                     /locus_tag="VHS97_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446906.1"
                     /translation="MRDHLVQRRRGANAGAERTGSPFRARLKQYSAENPSSDGQKDDF
                     NYDANWMKHSNGADEVEIEELMVTLRLYRAISKYQAVYGIGTPEMAAPFPSAGEAGVA
                     VGNASSEEPPHAA"
     gene            623..1120
                     /locus_tag="VHS97_01570"
     CDS             623..1120
                     /locus_tag="VHS97_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3446907.1"
                     /translation="MSATDVHDTSRDASVGQVDMKLEVLIIPVSDVDRSKEFYERLGW
                     RLDRDVAPMEGLRIVQFTPPGSGTSVTFGQGLTTSAPGSAEGELVVSDIEAAYDELVG
                     RGIEVSDIWHGPPFPVEARQPGPDPERTSYRSFLYFTDPDGNTWLVQEVTTRLWSAPR
                     PDERR"
     gene            complement(1700..>2059)
                     /locus_tag="VHS97_01575"
     CDS             complement(1700..>2059)
                     /locus_tag="VHS97_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3446908.1"
                     /translation="LEAERIKYAIRLPANQVLQERIAYLLTRPVGRPPNHVQRSHALA
                     YNLGNFPRTLAMPEPIKDWSLTSLKDKLIKIGAKVVSHGRYVIFQMAEVAIARQMFEE
                     ILRLIAELRPQPPPALA"
BASE COUNT          394 a          624 c          607 g          434 t
ORIGIN      
        1 tcggcccagc gtatcaaatc acgactcctg acagcgtcgg accttccaga atcggagttt
       61 atgcggcgtg aggcggttcc tccgagctcg cgttgcctac agcgaccccg gcctctccag
      121 ccgaaggaaa cggggcggcc atctcgggtg tcccgatccc gtagaccgcc tgatatttcg
      181 agatcgcccg atacagccgc aatgtaacca tcaattcctc gatttcgacc tcgtcggctc
      241 cgttgctgtg cttcatccag ttagcgtcgt aattaaagtc gtccttctgc ccatcggatg
      301 aggggttctc ggcactgtac tgttttagac gggcgcgaaa aggtgagccg gtgcgttcgg
      361 ctccggcatt tgcccctcgg cggcgctgca caaggtgatc gcgcattcga aatcgccctc
      421 tcgaaaatgt ttgatcgcag cctgtatctg tcgctccgcc gcgagccgtc tgcttgtctg
      481 aatcacacgt ccagccatgg ctcaccttgt aaagagtcga acagtgcgcc cttgcgaatt
      541 ctagtttcgc accagaaccc tcggagccgc cagacgttgc ggaactcaag gatttataaa
      601 aacgtggaag gttgaggtag cgatgagcgc caccgacgta cacgacacaa gccgcgacgc
      661 gagcgtcggg caggtcgaca tgaagctcga ggtcctgatc atccccgtgt ccgacgtcga
      721 ccgatccaag gagttctatg agcgcttggg ttggaggctc gaccgcgacg tcgccccgat
      781 ggagggcctt cgcatcgtcc agttcacacc cccgggctcg gggacctcgg tcacgttcgg
      841 ccagggactc acgacgtccg cgccaggctc ggccgagggg gagctggtcg tctccgacat
      901 cgaggccgcc tatgacgagc tcgtcggccg tggcatcgag gtgagtgaca tctggcacgg
      961 cccgccgttc ccggtcgagg cgcggcagcc gggccccgac cccgagcgca ccagctacag
     1021 gtcgttcttg tacttcaccg atcccgatgg caacacgtgg ctggtccagg aggtcacgac
     1081 gcggctctgg tccgctcccc ggccggatga acgccgctga gacgggctac cagagtcgtg
     1141 tttggtttca aatggtacaa ggaaataggc gctcacgaag gccacgacca ccagtaatga
     1201 cgtgatgata gcgcttatcg tcgaatatag gatcgctttg ttcaacagtg ccgcacgcgt
     1261 cttgaggcgg gggacatcgg acttcagggc tgctttgacc ttatcatcgt ccgctatggc
     1321 gttcaacgct tgcgacctgt cgatgacgcg gttcatgcgc gaaatgagca ccgagatgaa
     1381 ggcggcgact gcacccaaaa gaaaggcagg tgccgtcact tgcgaaatga cctgagacag
     1441 ctgtgttgca gtgggtgtgt ctggcaacgg cacgtctcgc cttacgaggt tgtaaaccga
     1501 gattctccag atgaatccca aaccggcatc aatgttcgcg ttttcccttc cctcttgcaa
     1561 gacagacgcg aggtgctgac cgctaccagc acctcgggta acccgaacgc ttggtcgagg
     1621 gtctgatccg gcccattttc cctggcattt ggacgcactc cttctgtcgg ttgctcttga
     1681 atgcatgaca tcgaacgtct cacgctagcg ctggtggtgg ctgcggccgt agctccgcga
     1741 tgagccgcaa aatctcttcg aacatttgcc gtgcgatggc gacctcggcc atctggaaga
     1801 taacatagcg gccgtggctc acgactttcg cgccgatctt gatcagtttg tccttcaagc
     1861 ttgtcagtga ccagtcctta atcggctcgg gcatcgccag cgtgcgcggg aaattgccga
     1921 ggttgtaggc gagcgcatga gaccgctgca catggttcgg aggtcgtccg accggccgcg
     1981 tcagcaaata tgcaatcctc tcctgcagga cttggttggc cggcagccgg atcgcatact
     2041 tgatccgctc cgcttcgag
//
LOCUS       DATAQL010000093         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4436906, whole genome shotgun sequence.
ACCESSION   DATAQL010000093 DATAQL010000000
VERSION     DATAQL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4436906"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1168)
                     /locus_tag="VHS97_01580"
     CDS             complement(65..1168)
                     /locus_tag="VHS97_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3446909.1"
                     /translation="MSDPLTWSPISLGRWFGTSVRVHITLIVFVVFQLLSTAVSQATK
                     GELSHLSQTACWLGLLLLALAVHELAHAVTAFWLDCDQDEVHIWPLGSLVGPSFVPRS
                     SEHILVAAAGPVSNGLFVLLTAIGLSVFGGAQFVWNPFGNEETVEHVLTHYGGAPRLA
                     SGKFALPLSPVWIAGWFGYLNYILLLANLFIPALPFDCGRMLRAYLSSTSVVSSRESI
                     AAPWTARAVGLLLLIIGVVRLVKWRTDGITLILLAVLIEHLVRAEARLLEDGGYFDDG
                     VFGYDFSEGYTSLESGAPKVRPYRESALKRWRRRRSELRRQRRIAREAAEERRMDEIL
                     EKLHREGRVALTDEENRFLVRVSARYRNRTKNT"
     gene            1370..>1788
                     /locus_tag="VHS97_01585"
     CDS             1370..>1788
                     /locus_tag="VHS97_01585"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3446910.1"
                     /translation="MIPIPTLILAMVINSTSPEICPTVAVPLGPRSYNIRVVTNDPAG
                     FGPFARAELARTWAGASCRSALIVTDLHIADLGVPATIQASLSDVGIAAAITVLPTGE
                     STKSLEQASRLYDELVKIKADRHTVIVGVGGGVIGDLA"
BASE COUNT          371 a          568 c          536 g          313 t
ORIGIN      
        1 tcggtcaagc acgtccgccg cactgcggta acgtcgcccc ggccatgcgt tcgctgcggt
       61 tcgatcacgt gtttttcgtc cggttgcgat agcgtgcgct cacgcggacg aggaaccgat
      121 tctcttcgtc cgtcagggcg acgcgccctt cgcggtgcag tttttcgaga atctcatcca
      181 tgcgccgctc ttcggccgcc tcgcgggcga tccgccgctg acggcgcagc tcggaccgac
      241 gacgccgcca ccgtttcagc gcgctttccc ggtaaggacg aaccttgggg gcaccgctct
      301 ccaggctcgt gtagccctcg gaaaagtcgt agccaaagac accgtcgtcg aaatagcctc
      361 cgtcctcgag cagacgagct tcagcccgga ccagatgctc aatcaacacg gccaacagga
      421 tcaaggtgat cccgtccgtg cgccacttca ccaggcggac aacgccgatg atcagtaaca
      481 gcaatccaac cgcccgcgct gtccacggag cagcgatgct ctcgcgggac gagacgactg
      541 atgtactcga caaataggcg cgcagcatcc ggccgcagtc gaaagggagc gcgggaatga
      601 acaggttggc aagaagcaag atgtaattga gatatccaaa ccaccctgcg atccagacag
      661 ggctcaaggg gagcgcgaat ttgcccgacg ccagcctggg tgccccgccg tagtgggtca
      721 ggacatgctc aacagtctct tcgttgccga acggattcca gacgaactga gctcctccga
      781 aaacgctcag gccgatggcc gtcaagagaa cgaaaagccc attcgacact gggccggcgg
      841 cggcgaccaa gatgtgctcg ctcgatcgag gcacgaacga gggtcctacc aggctaccca
      901 gcggccagat atggacctca tcctggtcgc aatcgagcca gaaggccgtg acggcatggg
      961 ccaactcgtg caccgccagc gcgagtaaga gcagacccag ccagcaggct gtttgcgata
     1021 aatgagagag ctcgcccttg gtggcctgcg agacggccgt cgacaagagc tggaatacca
     1081 cgaaaacgat cagggtaatg tgcacgcgga ccgaggttcc aaaccatcgg cccagactga
     1141 ttggcgacca ggtcagagga tcgctcatag tgccaacccg gattcgtacg cgatcgcgcc
     1201 tttggaatcg tcgctcgcca ctcttacact acctaatcta acagcgaaac tggttggtcg
     1261 caacgcgagc actcggccgc tccgcgcgcc gtcttgcccg tagcggcgcc cgggcgctgc
     1321 ttcttcgatc cgactgcgca tgctaaagtg aaacatcgtg aggttgacgg tgataccaat
     1381 tccgaccctg attcttgcaa tggttatcaa ctcgacgtcg cccgagattt gccccaccgt
     1441 ggccgtcccg ctcggcccgc ggtcgtacaa cattcgggtt gtcacgaacg atcccgcagg
     1501 gtttggacca ttcgctcgcg cggaactggc tcgaacctgg gcaggcgcat cctgccggtc
     1561 ggccctcatc gtgaccgacc tgcatatcgc cgacctcggc gtcccagcga ctattcaggc
     1621 gtcgctgtca gacgtcggga tcgcagcggc gatcaccgta ttgcccacgg gagaatcgac
     1681 aaaatcgctc gagcaggcgt cgcggctgta cgatgagctg gtgaagatca aggccgatcg
     1741 tcatacggtg attgtcggag tggggggggg cgtgatcggc gacctggc
//
LOCUS       DATAQL010000094         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_314575, whole genome shotgun sequence.
ACCESSION   DATAQL010000094 DATAQL010000000
VERSION     DATAQL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314575"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VHS97_01590"
     CDS             <1..328
                     /locus_tag="VHS97_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3446911.1"
                     /translation="LTPDCHSKAQNILRQVGLGHRLGHKPSKLSGGQQQRVAIARALI
                     KDPAVLLADEPTGNLDHKGGDEIFALLREQQDKRGCTLVVVTHDRRFIRPDDHVLEIE
                     DGRIAS"
     gene            463..1752
                     /locus_tag="VHS97_01595"
     CDS             463..1752
                     /locus_tag="VHS97_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3446912.1"
                     /translation="MQTTKKPQERLRAAVPRFALVLIPSAVLALAGLAASQDTKPQAR
                     SATKGSLPALKAKVAFPNLRFDRPIALASPDDGTNLLFVAEQHTATIWSFPNERKTAD
                     KKLFLPLRHPINKGNEEGLLGLAFHPKYRENKQFFIYYSANDSGRRHSVVSRFRASSS
                     DPRVADPASEERIWVSAEDPFENHNGGTIAFGPDGYLYITLGDGGAADDPLNSGQNPR
                     DWFASILRIDVDHPDAGKLYGIPADNPARRERKYSGWAPEVYCIGLRNVWKFSFDRKT
                     GELWAGDVGQNLWEMVHRIENGGNYGWSIREAFHPFQPQRRQRPNAASKIQPPIVEYP
                     HSPTADRPDSGLSITGGYVYRGKLNPALEGVYVYGDFETGRIWGLRYEQGKVLANGEL
                     IDVRRAPKLNIASFGEDAEGELYILAFDGRIHELVPL"
     gene            complement(1878..>2782)
                     /locus_tag="VHS97_01600"
     CDS             complement(1878..>2782)
                     /locus_tag="VHS97_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX3446913.1"
                     /translation="NDHALISDGVYGKTTSLVTRQLPRWGIDHDIFDPAAGPATLKSL
                     MKPETRLIYTETASNPLLKVADLDSMAAVARQAGIPLIVDNTFTPVICRPIDHGASLV
                     VHSVTKMIGGHSDLTLGAVVGAKNLVEQAKVIASTLGQTGNPFESWLALRGMATLCLR
                     FKRACDSSLELARRFESDGRVVQVYYPGLKSHPDHELALRILKDGFGAIVTIDLGSRS
                     NAQTFIKALDGIPFAPSLGDVQTTLSHPTTTSHRGQDDAQLARQAITPGMIRISVGIE
                     DAGDLWREFSHALGALKVAPAFRP"
BASE COUNT          568 a          834 c          849 g          531 t
ORIGIN      
        1 gctgacgcca gactgccact ccaaggcgca gaacattctc cggcaggtcg gcctggggca
       61 tcggctcggg cacaagccgt caaaactctc cggcggccag cagcagcggg tggccattgc
      121 gcgggcattg atcaaagatc cggccgttct gcttgccgat gagcccacgg gcaacctcga
      181 tcataagggg ggcgacgaga ttttcgccct cttgcgcgag cagcaagata aacgaggttg
      241 tactttggtc gtcgttaccc atgaccgccg cttcatccgt cccgacgacc acgtcctcga
      301 gatcgaagat ggccggatcg cgtcgtgacg cgggacaaaa actgggaaac gacgggcaac
      361 tgccctacaa tagcccgtgc cgaaagccgg cgagctggcc tcacggaacc gtttaaaacg
      421 acccgccgat tgttccctcc accgtttgaa ggaatgttgc gaatgcaaac gacgaagaag
      481 ccgcaagagc gcttgcgggc cgcagtcccc cgttttgccc tggtcttgat cccgtcggcc
      541 gtcctcgccc tggctggctt ggcagcttcg caagacacga agccgcaggc tcgatctgca
      601 accaagggat cgctgccggc cttgaaggcg aaagtcgctt ttcccaatct tcgcttcgac
      661 cgtcccatag cattggccag ccctgatgac ggcaccaacc tgttgttcgt ggcggaacag
      721 cacacggcca caatctggtc gtttcccaac gagcggaaaa cggccgacaa aaagcttttt
      781 ctcccgcttc gccatccgat caacaaaggc aatgaagaag ggttgcttgg cctggctttc
      841 catccgaaat acagggaaaa caagcaattc tttatctact actccgccaa cgattccggc
      901 cgccggcatt cggtcgtgtc tcggttccgc gcatcgagca gcgatccccg cgtcgccgat
      961 ccagcgagtg aagagcggat ctgggtgtcg gccgaagatc cgttcgaaaa ccacaacggc
     1021 ggcacgatcg ccttcggtcc ggacggatat ctctacatta cgctgggaga cggtggcgct
     1081 gccgacgacc ccctaaatag cggccaaaat ccccgcgatt ggttcgcgtc catactgagg
     1141 atcgacgtgg atcaccctga cgcgggcaag ctgtacggca tccccgcgga taaccccgcg
     1201 cggcgggaga ggaaatactc tggctgggct ccggaggtct attgcatcgg cctgcgcaac
     1261 gtctggaagt tcagctttga tcggaagacc ggcgagctgt gggcgggcga cgttggccag
     1321 aacctctggg agatggtcca tcgcatcgag aacgggggca attacggctg gagcatcagg
     1381 gaagcattcc atccgttcca gccccaacgg cgccagcggc ccaatgccgc ctccaagatc
     1441 cagccgccga tcgtcgagta tccccattcg ccgaccgccg accgaccaga cagcggcttg
     1501 agcatcaccg gtggatatgt ataccgcggc aagctgaatc ccgcgctcga gggcgtctac
     1561 gtctacggag atttcgagac cggacgaatc tggggcctgc gctatgaaca ggggaaagtc
     1621 ctcgcgaacg gcgagctgat cgacgtcagg cgcgcaccca agctgaatat cgcgtcattc
     1681 ggcgaagacg cggaggggga attgtatatc ctggcatttg acggccggat ccacgagctt
     1741 gtaccgctct aagtgcaccc gccgaagtcg tcacgcagcg aaggtcgagc tttctagccc
     1801 gacgttttac tggtgggcac aaagtgtcag gctggatagt ctggcataca caaatagttc
     1861 tgcggtctca cttagggtca ggggcgaaat gccggcgcca ctttcagagc gcccaaggca
     1921 tgactgaatt ctcgccacag gtcgccggcg tcttcgatgc cgaccgagat ccggatcatg
     1981 ccaggcgtga tcgcctggcg agcgagttgg gcatcgtctt gtccgcgatg gctggttgtg
     2041 gtggggtggc tgagcgtcgt ctgcacatcg cccaagctcg gggcgaacgg aatcccgtca
     2101 agcgccttga tgaatgtctg ggcgttggac cggctaccaa ggtcgattgt gacgattgcc
     2161 ccgaagccgt ctttgagaat gcggagcgca agctcatgat cggggtggct cttaagtccg
     2221 ggataataga cttggacgac acggccgtcc gattcgaacc ggcgtgccag ctcgagcgag
     2281 ctatcgcatg cgcgtttgaa tcggaggcag agagtcgcca tgccgcgcag ggccagccag
     2341 ctttcgaacg ggttgccggt ctggcccaga gtcgaggcga taaccttggc ttgctcgacg
     2401 agattcttgg ctccgacgac cgctccaagc gtcaggtcgc tgtggccgcc gatcatcttg
     2461 gtcaccgagt gcacgaccag actggcgcca tgatcgatcg gccggcagat caccggcgtg
     2521 aaggtgttat cgacgatcaa gggaataccg gcttgccgcg cgaccgctgc cattgagtcc
     2581 agatcggcca ccttgagtag tgggttcgag gctgtctcgg tgtaaatcaa ccgggtctcc
     2641 ggcttcatga gtgacttgag tgtcgccggg ccggcggccg ggtcaaagat gtcgtgatcg
     2701 atgccccagc gcggcaattg cctggtcaca agtgatgtcg tcttgccgta aactccatcg
     2761 gatatcaagg cgtgatcatt ct
//
LOCUS       DATAQL010000095         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5352819, whole genome shotgun sequence.
ACCESSION   DATAQL010000095 DATAQL010000000
VERSION     DATAQL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5352819"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VHS97_01605"
     CDS             complement(<1..1179)
                     /locus_tag="VHS97_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX3446914.1"
                     /translation="MATTVDEPKAVVLRDDDTVGVAARPIPRGFTLQLGGRTIEVREP
                     IGLGHKVALVDIRPGEPVRKYGQIIGFAAKPIAAGSLVHVHNLEADLFERDYAFASER
                     PSSALPDRLRTFRGYLRPDGRVGTRNYIAVVSTVNCSASTSRYVADRFRDGSWRRDFP
                     NIDGVFAITHKGGCGLPFEGLDHQILERVLAGFAHHPNVAAYVIVGLGCEVSYASHLV
                     ETQHLITLGRANGNGQKQPAHRPRTLNIQEEGGITRTVEAAAKAVYELMPEANSWVRT
                     EQPASKLCLAMECGGSDGNSGVTANPALGAAADLLVAQGGTAILGETTEIYGAEHLLT
                     RRAVSRAVGEKLVDRIKWWEWYTGVFGARIDNNPSPGNKAGGLTTIYEKSLGALAKAG
                     S"
     gene            1353..2162
                     /locus_tag="VHS97_01610"
     CDS             1353..2162
                     /locus_tag="VHS97_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3446915.1"
                     /translation="MPSPFPGMDPYLEQPSGWTDVHVELISATRAALKALVGPKYLVR
                     IEERIYISNDNDPGRIDLVPDVQVSSRDVGARPLTMAADGGTEVAEPLVLETLVDEKI
                     REAYIKVVDAQRNKVVTVIEILSPDNKVAKSQGLKSFRMKRVEVMRSASHWVEIDLLR
                     RGVSLPLRKRIRPHDYFVHVSPTHRRPLGLVWPIRLSQRLPVIPIPLRAGDDDAPLDL
                     QSVLDTAYDHAGYDTDINYTKEPVPPLSPPWSEWSDRWLREKGLRPQEAIE"
     gene            complement(2169..2975)
                     /locus_tag="VHS97_01615"
     CDS             complement(2169..2975)
                     /locus_tag="VHS97_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX3446916.1"
                     /translation="MGIRISQVDAFTKRRFAGNPAAVCVLAEPADVSWMQDVAAEMNL
                     SETAFVHRLEHGTKYHLRWFTPRNEVDLCGHATLATAHILWEEGHLPAGEPALFETRS
                     GLLTARSGPDGIELDFPSEPVHEEVTDAGKLTELQSALGASVQYAGRNRFDLLVELET
                     EELVRTLRPDIRRLGQFPVRGVIVTSRSNSRDCDFVSRFFAPQVGIDEDPVCGSAHCC
                     LGPYWALKLGRLSLIAHQVSCRGGVVKIRVEGNRVALTGHAVTVLRGELV"
     gene            complement(3120..3320)
                     /locus_tag="VHS97_01620"
     CDS             complement(3120..3320)
                     /locus_tag="VHS97_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446917.1"
                     /translation="MARLAKSGASPEQVTKELYAVALCRDPNSSELETATRHLTSSKD
                     FRLAIEDLGWVLINSKEFLFRH"
     gene            complement(3328..>3542)
                     /locus_tag="VHS97_01625"
     CDS             complement(3328..>3542)
                     /locus_tag="VHS97_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446918.1"
                     /translation="VTATTTAFDGLPRGARATQIPDGKMENPFLKTFGRPARELACEC
                     ERESDSNLSQALQLIGGDRQRQAPGR"
BASE COUNT          701 a         1099 c         1061 g          681 t
ORIGIN      
        1 tgaaccagcc ttcgcgagcg ctccgagcga cttttcgtag atcgtggtca agccgcccgc
       61 cttattgccc ggcgaggggt tgttgtcgat ccgggcacca aacacgccgg tataccactc
      121 ccaccatttg atccgatcga cgagcttctc ccccaccgcg cgcgaaacgg cccggcgcgt
      181 cagcaggtgt tccgcgccgt agatttcggt cgtctcaccc aggatggcag ttccaccctg
      241 agcgaccagc agatctgccg ccgctccgag cgccgggttg gcggttacgc ccgagttacc
      301 atcggaccca ccgcactcca tcgccaggca aagctttgag gccggctgct cggtccgtac
      361 ccacgaattc gcctcgggca taagctcgta cacagctttt gccgccgcct cgaccgtgcg
      421 ggtgatcccc ccctcctcct ggatattgag ggtccgcggc cggtgagccg gctgcttctg
      481 cccgttgccg ttggcccgcc ccaaagtgat gaggtgttgc gtctcgacca ggtgcgaggc
      541 atagctcact tcgcatccga gcccgacgat cacgtaggcc gccacgttag gatggtgggc
      601 aaagccggcg agaacgcgct caaggatctg atggtcgaga ccttcaaaag ggagcccaca
      661 gccccctttg tgggtgatgg caaaaacgcc gtcgatattg gggaaatccc gtcgccatga
      721 cccatctcga aatcggtcgg cgacatagcg agacgtgctg gcggaacagt tgaccgtgct
      781 gaccacggcg atgtagttcc gcgttcccac ccgcccgtcc gggcgcaggt agccacgaaa
      841 tgttctgagc cgatccggca acgctgacga gggccgctcg gacgcaaagg cgtagtcgcg
      901 ctcgaagagg tcggcctcga ggttgtgcac atgcacgagc gagccggccg cgattggttt
      961 cgcagcaaaa ccgatgattt ggccatactt tcgcaccggc tcgcctggtc tgatatctac
     1021 gagagcgacc ttgtggccta ggccgatcgg ctcgcgaacc tcgatcgtcc tcccccccaa
     1081 ttgcaatgta aaaccgcgcg gaatcggtct ggccgccacg ccgactgtgt cgtcgtctct
     1141 cagaacgacg gctttgggct catcgaccgt tgtcgccatg tccgacctct gctagcgaac
     1201 gcacttttgc aaattctccc cgataatata gtctagtggt tttcccagtc gttagaaagc
     1261 cgctctgaac cgacatgttg cggcggtata attcatcgtg ccgtcgtcct cgcgtgcgac
     1321 ggaactgccg aaacgcgctg ggaggatcac cgatgccttc gccatttccc ggtatggacc
     1381 cgtatctaga gcagccctct ggatggacgg acgtgcatgt cgagctgata tcggcgacgc
     1441 gggcggcgct caaagcgctg gtcggcccta agtacctcgt tcggatcgaa gagcgaatct
     1501 acatatcaaa tgacaacgac cccgggagaa tcgacttggt gcccgatgtt caagtctcat
     1561 cccgagacgt cggcgctcga cctctgacga tggcggccga cggcggtacc gaagttgcgg
     1621 aaccgctcgt gctcgagaca cttgtcgacg aaaagattcg cgaagcttac atcaaagtgg
     1681 tcgacgcgca gcgtaacaag gttgtgacgg tcatcgaaat cctgagcccc gataacaagg
     1741 tggcaaaatc acaaggactc aagagcttcc gaatgaagcg tgtcgaggtc atgaggtcgg
     1801 cgagccattg ggtcgagatt gatttgcttc gccggggcgt ttcgctgccg ctgagaaaac
     1861 gaatccgtcc ccacgattat tttgtacacg tctcccctac tcaccgccgg cccctgggtc
     1921 tagtctggcc aatacgtttg tcgcagcgcc tcccagttat tccaattccg cttcgcgccg
     1981 gcgacgatga cgctccgctc gatctccagt cggtcctgga cacagcctac gatcatgccg
     2041 gctatgacac cgacatcaat tacacaaaag agccagtccc gcctctgagc ccaccgtggt
     2101 ccgagtggtc cgaccggtgg ttgcgggaaa aagggctgcg accgcaagaa gcaatcgaat
     2161 gagggaatct acaccaactc gcctcgcaag accgtcaccg catgaccggt caaggcgacg
     2221 cggtttcctt cgacgcggat cttgaccact ccgccgcggc aagaaacctg gtgggctatc
     2281 agtgacagac gaccgagctt aagggcccag tacgggccca gacagcagtg tgccgagccg
     2341 cagaccgggt cttcgtcgat gcccacctga ggtgcgaaga accgggaaac gaagtcgcaa
     2401 tcacgcgagt tggaacggct ggtgacgatc actccgcgga ctgggaattg tcccagccgt
     2461 cgaatatcgg ggcgcaaggt tcgaacgagc tcctcggtct cgagctcgac caaaagatcg
     2521 aaccgattgc gaccggcata ttgcacggag gcccccaggg ccgactgcag ctcggtgagc
     2581 ttgcccgcgt ctgtgacctc ttcatggact ggttcagacg gaaaatccag ctcaattcca
     2641 tcggggccgc ttcgcgcggt caaaaggccg ctgcgcgtct cgaagagggc gggctcaccg
     2701 gccggcaggt ggccttcttc ccagagaatg tgggcggtcg ccagagtggc atgaccacat
     2761 aggtcgacct cgttccgggg cgtgaaccag cgcaaatgat acttcgtgcc gtgctcgaga
     2821 cgatgcacga aggccgtttc ggagaggttc atctccgcgg caacgtcttg catccagctc
     2881 acgtccgccg gctcggcaag aacgcagact gcggccggat taccggcaaa ccttcgtttg
     2941 gtgaacgcgt cgacctggct gatgcggata cccatgagcg gtcgctccgg ctgagaggcg
     3001 aaaactgtcc ctcctcgatg gatcgctatt atagagccgc caacgcccga acggttcggt
     3061 gtcggtacga atgcgatcga tgtcgagatc gactgagcaa tgcgcgcctg ttctccagct
     3121 caatggcgga acaggaattc cttggagttg atcaacaccc agccaaggtc ctcgatggcc
     3181 agccggaaat ctttcgaaga ggtgaggtgc ctggtggccg tctccagctc ggacgagttc
     3241 ggatcccggc aaagtgccac ggcatagagc tctttggtga cctgctcggg cgatgcgccg
     3301 cttttagcca ggcgagccat tcggccgcta tcgtcccgga gcttgccgtt gacggtcgcc
     3361 gccgatcaac tgcagggcct gcgagagatt cgaatcgctt tcgcgctcgc attcgcaagc
     3421 cagctcacgg gcagggcggc caaatgtctt caagaaaggg ttctccatct tgccgtcggg
     3481 gatctgggtg gcgcgagcgc cgcggggaag tccgtcgaag gctgtcgtcg tggcggtgac
     3541 cg
//
LOCUS       DATAQL010000096         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2910076, whole genome shotgun sequence.
ACCESSION   DATAQL010000096 DATAQL010000000
VERSION     DATAQL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2910076"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            828..>3083
                     /locus_tag="VHS97_01630"
     CDS             828..>3083
                     /locus_tag="VHS97_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446919.1"
                     /translation="MTVRWKPLVILSGLFLLVALIGVVAITLTLVPRSSQSFLRRARI
                     AHGAGRFEEAEIDYKQALQFDAKDATVHDEFAGFYRDWAKHAAADKRALLLNERHNHL
                     ESAVKFDKAIKAPRLELLRDALRDDLVPESNYWAKEVLNVEPENLDAHFVLALEALEN
                     RTPNVPDVRRHLEVLEKKKAPAIRRLLVRAKLAEATGDKAAREEAFEQARAIKLGPES
                     NPADQMAGLRIVSLTVRVEADPARLDQDVSRMLRQVKDLDKTDELVPARVARVRTLLD
                     QTQRALILRSARVPDVVKKVIDRQVDAIEIDLESIFKLALAGEHTSDLQTYVAYADHL
                     RFRQQRDRCLQVVDQGLKLPQASKPTAAQAVMRLHVVAVEMALSRGDDQARFDKAAPH
                     IQALLDCPDQLFQGFGHLFAGSVDLDRSGVIRVTEGGAGGAATKESPAHLRTSALHHL
                     KIAAALMPEIAEAQARYGVALVLAGEQNLGRQFLQTALRLGSLDSQYQLWAAWTILQA
                     GYPEDAEPIVSALLRQVATGDAPRELEASLHLLSGEIHQAKRSPEELNKAVAEFDKAL
                     ATGHEMSTTAVVRLAQIDVQLGRYDRALARIDALTGQGKGSAAAEQLAVLTLEQQGKA
                     AEARSRLRVARNRYPTGADLAGIDAALLDKDGKHAEADKVLEQFLAAGPDNPTLVMMR
                     AQIQAESLKDFAKARSLLLAVSEKTENSAPLVQLADLELARNQLDQAQEVIAKIRNRW
                     RESATSDVLEAQ"
BASE COUNT          635 a          943 c          916 g          589 t
ORIGIN      
        1 ctcggaaaga ttaaaaaact gcgagacggc ccgctctgca aatgaaacgc accgtccaga
       61 gtatgaaccc acgcgtgcga tcaagattcg cacctcctat ctcaggcgct ccgccgcgac
      121 ttacgtcacg ggcggcggtt tgcgcatctc cgtcacaatt cacaggccac agggtgattt
      181 acacaggcaa atgcaatcga tgtaaaggtc cggcaattgg cgacttgcgg ctgagcaatc
      241 gcgacgtcga tgagggcgag ttggcactga agacctttcc aggagtgcca ctcgttttat
      301 gacgtaattt tgtagactcg cccgcgcact gccggtcaga aggatcgccg tccggactgt
      361 aattcgacaa acctcggctt gcgggtcggc ttcgccgacc tcaaaccgcc gtggcacccg
      421 tttctactaa cgcaggttat gccagtctcc gttggcgaac ccagccgcac ggtagtcgcg
      481 cggattcccg cgaaagttag cttgatgtct gagcgaggaa accctatcct gaccccagcc
      541 tgatgccggg atagaacggc aatggattgc gacctacttg aggtatcagg gcaaatggta
      601 ttgcccctcc cgagtctcga tttctcgaac ctgagtcgcc accgacctgg atcgggtctt
      661 cgcgcatccc atcgcttcat ttcttccggc tcgttaaagg ccgtcagtct cctcccgatt
      721 tccatcaccg cgcgttcttc ttccgcaagg cgacccggat ggtcgccgtg gcggattggt
      781 tgaccggccg cgcggttcgg tgcctagagc caaaggagag tcgcgcgatg accgttcgct
      841 ggaagcccct ggtaattctt tctggtctat tcctgctcgt ggcattgatc ggcgtcgtcg
      901 ccatcaccct gacattggtg ccacgctcat cgcaaagctt cctcaggcgc gctcgcatag
      961 cccacggtgc cgggcgcttc gaggaggccg agattgacta taaacaagcc ctccagttcg
     1021 acgccaagga cgccacggtt cacgacgaat ttgccggttt ctatcgcgat tgggccaagc
     1081 acgctgccgc cgacaagcga gctttgctct tgaacgagcg gcacaatcac ctggagagcg
     1141 cagtcaagtt cgataaagcg atcaaggcac cccgattgga gctgctccga gacgcccttc
     1201 gcgacgacct cgtgcctgaa tcgaactatt gggccaagga agtcctgaat gtcgagccgg
     1261 aaaacctcga cgcgcatttt gtgctggcgc tcgaggcgct tgaaaaccgt acgcctaacg
     1321 tgcctgacgt gcggcgccat ctcgaggtcc tcgaaaagaa gaaggcaccg gccatccggc
     1381 ggctcttggt tcgagcaaag ctcgctgaag ctaccggcga caaggcggcg cgcgaagaag
     1441 cttttgagca ggcccgagcg atcaagcttg ggcccgagtc gaatccggcc gatcagatgg
     1501 ccggcctacg gatcgtatcg ctgactgtcc gggtcgaggc cgatccggct cgattggatc
     1561 aagacgtgag ccgcatgctc cgccaagtca aagatctgga caagaccgat gagctcgtcc
     1621 cggcgcgggt cgcacgggtg cggacgctgc tcgaccagac tcagagagcc ttgattcttc
     1681 gttcagcgcg cgtccctgat gtcgtcaaga aggtgatcga tcgtcaggtc gatgcgatcg
     1741 aaatcgacct cgaatcgatc ttcaagcttg cgctcgccgg agagcacact tccgacctgc
     1801 aaacctatgt ggcatacgcc gatcatcttc ggtttcgcca gcagcgagac cgatgcctcc
     1861 aggtcgtcga ccaggggctc aagctccccc aggcgtcgaa accaacggcg gcccaggcgg
     1921 tcatgcggct acacgtcgtt gctgtcgaga tggccttgtc gcgcggggat gaccaggctc
     1981 gcttcgacaa ggcggcgccg cacattcagg ctctcttgga ttgtcccgac cagctctttc
     2041 aaggattcgg ccacctgttc gccggttcgg tcgacctgga tcgctcgggc gtaatccgcg
     2101 tgacggaggg cggcgctggc ggcgccgcaa ccaaggagag tccggctcat ctgcggacga
     2161 gcgccttgca tcatttgaag atcgccgcgg ccctgatgcc ggagatcgcg gaagcccagg
     2221 cacgctacgg cgtggcattg gtgttggcgg gcgagcaaaa cctggggcgt cagtttttgc
     2281 agaccgcatt gcgattgggg agcctcgatt cgcaatatca gctctgggcc gcgtggacca
     2341 ttttgcaggc aggctatccg gaggatgccg aacccatcgt gagcgctcta ttgcggcaag
     2401 tcgccaccgg cgatgcgccg cgcgagctcg aggcgagcct gcacctcctg agtggcgaga
     2461 tccaccaggc aaaacgatcc cccgaggaat tgaacaaggc cgtcgcggag ttcgataagg
     2521 ctttggcgac cggtcacgag atgagcacga ccgcggttgt gaggctggcg cagatcgacg
     2581 ttcaactcgg ccgatatgac cgtgcgttag ctcgaatcga cgcactcacg ggccaaggaa
     2641 aaggaagcgc tgcggccgaa cagcttgccg ttttgactct tgagcaacaa ggcaaggcgg
     2701 ccgaggccag gtcgcgatta cgcgtggctc gcaaccgcta tcccaccgga gccgatctcg
     2761 ccgggatcga tgcggcatta ctggacaagg atgggaaaca cgccgaagct gataaggtcc
     2821 tggaacaatt cctcgccgcc ggacccgaca acccgactct ggtcatgatg cgtgcccaga
     2881 tccaggctga atcgctcaaa gatttcgcca aagcgcgatc gctgcttttg gccgtctcgg
     2941 aaaaaaccga gaattcggct cccctcgttc aattagccga tctcgagctc gcgcggaatc
     3001 agcttgacca ggcccaagag gtcatcgcca agatccgcaa tcgatggaga gaatccgcca
     3061 caagcgacgt gctcgaagcc cag
//
LOCUS       DATAQL010000097         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2451987, whole genome shotgun sequence.
ACCESSION   DATAQL010000097 DATAQL010000000
VERSION     DATAQL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451987"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VHS97_01635"
     CDS             <1..709
                     /locus_tag="VHS97_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446920.1"
                     /translation="ADLDWNTIFQLSRKWANRFEITLAQDFVDHLDDSADGETGRLLF
                     EVEGTDAATTAMATEFAKAIQGKMVLGLVTAAGIPAKPAGPTVACKIRLSAGEASVQI
                     SGSDATAMKWLPYGKFSQPVVQENGKLDVVKFADALADGILNRLVRAQVLKGSAQREK
                     GKLIYQIRIENASPLILNGLAMLGTASEQDEVAKVLSGIAISPHRTMLVPMHEDVVKS
                     LGLKKGIKVLALDLSGL"
     gene            complement(846..2084)
                     /locus_tag="VHS97_01640"
     CDS             complement(846..2084)
                     /locus_tag="VHS97_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3446921.1"
                     /translation="MRFQQTTLDNGLQVIAELNDQAHSVAAGFFVKTGSRDESAELAG
                     VSHFLEHMTFKGTPTRDAVAVNRDFDRVGAKHNAQTSEEDTFYHVTCLPEYLPQAFDV
                     LSDILRPTLRGDDFETEKQVIIEEIRMYLDNPMSVAYEAAKATHFGEHPLGNSILGTV
                     ESIAGLQADRMRAYFVQRYCPSNIVLAFAGKGEWNSLVNLAQAHCGAWQKGSADRQAV
                     PVRGTGSFRAILRAEDQQQTVVAVCDAPPLESEDRYAAHLLATILGDHTGSRLYWTLI
                     DPGLADGAELSYQTYNQAGAFFSFLSCEPSATQANLDRIARVYRTAIAQGVSEEELVQ
                     AKNKVLARSVLRSERPMGRLATFGFHWMYQHTYISIDQELDAFNKVGLADVRRLLEDW
                     PLWPTTIVSVGPTTDVHVPE"
     gene            complement(2163..3434)
                     /locus_tag="VHS97_01645"
     CDS             complement(2163..3434)
                     /locus_tag="VHS97_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3446922.1"
                     /translation="MAAALSSMLEQHTFPNGLVLVAENMPGVQSAAFTLLLPAGAAYE
                     GADGLNLKGGAATMAAEWISRGAGPRDSRELLTALDNLGVSHAESAQSLHTSLAVATL
                     GRNLEPALEIIADMVLRPHLDDDEVEPIRALCSQNLRSLDDDPGTKVIYELRRRHFPD
                     PWGRPSPGTAEGVAALTTTDLRHFIGQTYRPNGAILGVAGSIDWSRLRDHAGRLFEGW
                     KPKPEPRVPERPGGPLRDHILRETQQIQIALASPAATVADTDYYRARAATAILGGYSS
                     ARLFTEVREKRGLCYSVYASYESQLDRAAMLCYAGTSADRAQQTLDVMLAEIVRLGRG
                     GIALDELDTMRAGLKSSLIMSQESSMSRSGSLASDWYFLGRVRPIEEIAASLDALTPE
                     AVSDYAARLQDTSNLTILTLGPTALQFAVVP"
BASE COUNT          730 a         1084 c         1161 g          730 t
ORIGIN      
        1 cgccgatctc gactggaaca cgattttcca gttgtcgcga aagtgggcca atcgctttga
       61 aatcaccttg gcccaggact tcgtcgatca tctcgacgac tcggctgacg gtgagacggg
      121 acgattgctc ttcgaggtgg aagggaccga cgcggctacc acggccatgg cgacagaatt
      181 tgccaaagcc atccagggca agatggtgct cggcctcgtg accgctgcgg ggatacccgc
      241 aaagcccgct ggacccaccg tcgcctgcaa gattcgtctg agcgccggcg aggcgtcggt
      301 gcagattagc ggcagcgacg caacggcaat gaagtggctt ccctacggca aattctcgca
      361 accggtggtt caagagaacg gcaagctcga cgtcgtgaaa tttgccgatg cgttagctga
      421 tggcatcctc aaccgcttgg tccgcgctca ggtgttgaag ggctcggccc agcgtgagaa
      481 gggcaagctc atttaccaga tccgaatcga gaacgcgtcg cctctgatct tgaacgggct
      541 ggcgatgctc ggaaccgcga gcgagcaaga cgaagtcgcc aaggtattgt cggggatagc
      601 catctcgccg caccgaacca tgctcgttcc catgcacgaa gatgtcgtca aatccctggg
      661 cctgaaaaaa ggcatcaagg tgttggcgct tgacctgagc gggctgtgaa cccagtccga
      721 taacgggctc aagtgattgc ggatccaagg tgggtcagcg cgagcgattt caagtcaccg
      781 accccgggcg agtcgatcgt ccggggcggt tttgttttcg aacggtgagc cggaacggtg
      841 gccgattatt ccggcacgtg gacgtcggtc gtgggaccga ccgagacgat cgtcgtcggc
      901 cagaggggcc aatcctcgag cagacggcgc acatcggcca ggcctacttt gttaaatgcg
      961 tctagctcct gatcgatcga gatgtacgta tgctggtaca tccagtggaa gccgaaggtg
     1021 gcaagtcggc ccatcggtcg ttcgcttcgg agtacggagc gcgccaagac cttgttcttg
     1081 gcctgaacca gctcctcttc gctcaccccc tgggcgatcg ccgtgcggta aacgcgcgca
     1141 atccgatcga ggttggcctg ggttgcggag ggctcgcagc tcaagaaaga gaagaaggcc
     1201 ccggcttggt tgtaggtctg ataagaaagc tcggcgccgt cggccagacc gggatcgatc
     1261 aaggtccagt aaagccgtga gccggtatga tcccccaaga tcgtcgcgag cagatgcgcc
     1321 gcgtaccgat cttcgctctc gagcggaggc gcatcacaca cggccacgac cgtttgctgc
     1381 tgatcttcgg cgcgaagaat cgcccggaat gatccggtgc cacgtaccgg gaccgcttgt
     1441 cggtcggccg aacctttctg ccacgcgccg caatgggcct gggcaagatt cacgagcgaa
     1501 ttccattccc ccttgccggc aaatgcaagc acgatgttcg atgggcaata ccgttggacg
     1561 aagtacgcgc gcatccggtc agcctggagg cccgcaatcg actcgactgt gcccaggatg
     1621 ctgttaccga gcggatgttc gccaaaatgg gtggccttgg cagcctcgta tgcgactgac
     1681 atcgggttgt ccaggtacat ccggatctct tcgatgatca cttgcttttc ggtctcgaag
     1741 tcgtcgccgc gaagagtggg ccggagaatg tcggagagta cgtcgaatgc ctgcggaaga
     1801 tattccggca gacacgtcac gtggtagaaa gtgtcttcct ccgaagtctg agcgttgtgc
     1861 ttggctccca ctcggtcgaa gtcgcgatta accgccacgg catcgcgggt tggtgtaccc
     1921 ttgaacgtca tgtgttcgag gaagtgagaa acgccggcca gctcggctga ctcatcccgg
     1981 cttccggtct taacgaagaa gccggcggcc acagaatgcg cctggtcatt cagctcggcg
     2041 atcacctgca gcccgttgtc cagcgtcgtc tgttggaatc gcatcagagt caacctgtgt
     2101 gtggtgagag gtctctggat cgacgaagtc acgcatggtc cgcggacgac cttcgggtaa
     2161 agtcagggca caacagcgaa ttggagcgcg gtcggaccga gcgtgagaat tgtcaggttg
     2221 ctggtatcct gaagccgcgc ggcataatcg gacacggcct caggggtcaa ggcgtcgagc
     2281 gatgcggcga tttcttcgat cgggcggact ctgcccagaa aataccaatc ggaagccagc
     2341 gatcccgagc ggctcatgct cgattcttgg gacatgatca gcgagctctt gaggccggct
     2401 cgcatggtgt caagctcgtc gagggcaatt ccccctcggc cgaggcggac aatttcggcc
     2461 agcatgacgt ccagggtctg ctgcgccctg tcagccgagg tcccggcgta acacagcatg
     2521 gcggctcgat cgagctgact ttcatagctg gcgtacaccg agtagcacag cccgcgtttc
     2581 tcccgaactt cggtgaaaag ccgcgccgac gaataacctc ccaggatcgc agtggcggct
     2641 cgcgcccggt aatagtcggt gtcggcgacc gtcgcggcag gagatgccaa agcaatctga
     2701 atttgctgcg tctctcgcaa gatatgatcg cgaagcggtc cgccgggacg ttccggaacg
     2761 cgtggttcgg gcttcggttt ccagccctcg aacaaccggc cagcgtgatc gcgcagtcgc
     2821 gaccaatcga tcgagccggc aactccgagg atcgcgccgt tgggtctgta ggtctggccg
     2881 atgaagtggc gcaaatccgt ggtcgtaagc gcggcaactc cttccgccgt ccctggcgac
     2941 ggtcgtcccc acggatcggg gaagtggcgg cggcgcagtt cgtagatcac ctttgtcccg
     3001 gggtcgtcat cgaggctacg caagttctgc gagcagagag ctcggatcgg ctccacctcg
     3061 tcgtcgtcga ggtgaggccg gagcaccatg tcggcgatga tttcgagggc tggctccaga
     3121 ttgcggccca aggtcgctac agcgagtgag gtgtgcagcg attgggcgct ttccgcgtga
     3181 ctgacgccca gattgtcaag tgcggtcagg agctcacggc tgtcgcgcgg gccggccccg
     3241 cgcgaaatcc actccgccgc catcgtcgcc gcgccacctt tcagattcag tccatcggcc
     3301 ccctcgtagg ctgctcccgc gggcaaaagt aatgtgaacg ccgccgactg gacgcccggc
     3361 atgttctcgg cgaccagcac cagcccgttg ggaaaggtgt gttgttccag catggaagac
     3421 aatgctgcgg ccaaggggac ctcgaacgtt tttcatggac atcccgccgt cattgcacgg
     3481 gccatccgtg gcaagcggcc agaagtcgtc gcgacctgct tcgaccgttg gacggtccga
     3541 atccctgtca ttgaacgtga gagcctacca gaatcttccc tgcatgttga agagtgatca
     3601 agtatcctcg ccattttcgg agaggtttcg cgcattcgat atcgctttct taggggaagc
     3661 aattgccaaa agacgctatt atctataggg tcgcgcctcg ttatc
//
LOCUS       DATAQL010000098         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1689411, whole genome shotgun sequence.
ACCESSION   DATAQL010000098 DATAQL010000000
VERSION     DATAQL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689411"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1476
                     /locus_tag="VHS97_01650"
     CDS             226..1476
                     /locus_tag="VHS97_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446923.1"
                     /translation="MRFFLALTLALVTPGQTERGQGANSRMSSSDDVRVPYNRWQHGP
                     PADPGFFPIAVWLQDPRNAGQYKRAGFNLYVGLWEGPTEAQLASLKAAGMPVVCDQNR
                     VGLAHRDDPTIVGWMHGDEPDNAQEIRDEKTGRRHYGPPVAPAKVVANYERLRAADPT
                     RPVMLNLGQGVANDEWKGRGPGASLDDYPAYIRGADIVSFDVYPVADVEKPNGADFLW
                     YVPKGVDRLLKWTGGRKPVWNCIECTRIHNPAAKATPAQVKAEVWMALIHGSRGLIYF
                     VHQFKPQFNEHALLDDAEMLAAVTSLNHEITMLAPLLNSPTVVDGGTVRSSDPEVPID
                     LMVKRRSDATYVFAVGMRNRPARGSFVLRGLPALASAQVHGEDRALTIRDGQFADDFA
                     AYGVHIYKITAAGSASSTTNSLRP"
BASE COUNT          312 a          480 c          460 g          292 t
ORIGIN      
        1 gaagccgagt atgctgatgg gccagcgaac ccgcttctat gggttaccat tacggccgca
       61 cgatttcaat gagcgacgag gagacaagaa tgcagtcact ccctggggac agagcgatgc
      121 gtagatcggg cgcgatggtt ctccatgccg tggttttgct tgcttcaatc tgaatctgca
      181 aacgggaaca caatactgag tcccaagaaa cctcagggtt ttttcatgcg ttttttccta
      241 gccttgacgc tcgcgctcgt cactccagga cagaccgagc ggggtcaagg ggccaattct
      301 cgaatgtctt cgtccgatga cgttcgagtg ccctacaatc gctggcaaca cggcccgccg
      361 gccgacccgg gctttttccc gatcgccgtc tggcttcagg atccgcgcaa cgcggggcaa
      421 tacaagcggg cggggttcaa tctctacgtc ggtctgtggg agggtccaac cgaggcccaa
      481 ctcgcgtcat tgaaagccgc cgggatgccg gtcgtttgcg accaaaaccg cgtcggtctg
      541 gctcaccgag acgacccgac catcgtaggc tggatgcacg gcgacgaacc cgataatgcc
      601 caggaaattc gcgacgagaa aacgggccgg cgccattatg gaccgccggt cgcaccggcc
      661 aaggtcgttg ccaactacga gcgcctgcgc gcagccgacc cgacgcgacc ggtgatgctc
      721 aaccttggac agggcgttgc caacgacgag tggaaagggc gcggacccgg ggccagcctc
      781 gacgattacc cggcttacat tcgcggcgcc gacatcgtct cgttcgacgt ctatccagtc
      841 gctgacgtcg aaaaacccaa cggagccgac ttcctgtggt atgttcccaa aggtgtcgat
      901 cgccttttga agtggaccgg cggccgcaag ccggtatgga attgcatcga atgcacgcgg
      961 atccataacc ccgcggcgaa ggccacgccc gcccaggtca aggccgaagt gtggatggcc
     1021 ttgattcacg ggtcgcgggg cctcatctac ttcgtccacc agttcaagcc ccaattcaat
     1081 gagcatgcgc tattggacga cgcggaaatg ctggcggccg tcacctccct caaccatgag
     1141 ataacaatgt tggctccgct tttgaacagc ccgacggtcg tggatggcgg aacagtccgc
     1201 tcatccgatc ccgaggtgcc cattgatctc atggtcaagc ggcgatcgga cgcaacttac
     1261 gtcttcgccg tcggcatgcg caatcggcca gcgcgggggt catttgtgct ccggggccta
     1321 cctgccctgg catcggccca ggtacacggc gaggaccgcg cgctcacgat cagggacggc
     1381 cagtttgccg acgacttcgc agcctacggc gtgcacattt acaagatcac ggcagccggt
     1441 tctgcgagct caacaaccaa ttcactgcgc ccgtgagttt gggtttggcg accgcaacga
     1501 ggttggccag tccgtcaaac gccaacgggc tgcttgagcg cctt
//
LOCUS       DATAQL010000099         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3826970, whole genome shotgun sequence.
ACCESSION   DATAQL010000099 DATAQL010000000
VERSION     DATAQL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3826970"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1376)
                     /gene="dgoD"
                     /locus_tag="VHS97_01655"
     CDS             complement(120..1376)
                     /gene="dgoD"
                     /locus_tag="VHS97_01655"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEX3446924.1"
                     /translation="MKTRKIASGLINRRHVVAASLGGLGLAALDRVEATAQNPVSTEA
                     PIAAKDPLRVTKLETLFVKPRWLFLKVYTNAGIVGLGEPLVEGRAATVATAVKEIEPY
                     LVGKDPRQVVHHWQAIYRHAFYRGGPVLTSALSGVDMALWDIKGKALGVPVYELLGGP
                     TRRRVRVYAHARNVDGIKRGLAQGFTAFKTNPASRRPARYVETPAEVRYAAEKFAELR
                     KAAGDDIDIAVDFHGAISPATAKLLIKALEPFQPMFIEEPCQAQNHDIMAELARGTHL
                     PIATGERVFTKWGFREVLEKRAATILQPDLCHAGGITEVRLIAGIAEAYYAAIAPHNP
                     LGPISLAAGVQIAASIPNFFCQEQVSLGNGYLKKPFVVKNGYLELPTGPGLGIELDDN
                     AMAAQIGHDWKNREAYDADDGSVMDW"
     gene            complement(1639..2853)
                     /locus_tag="VHS97_01660"
     CDS             complement(1639..2853)
                     /locus_tag="VHS97_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3446925.1"
                     /translation="MNRLQLAVASTALLFALNTASIKGDEPRWKQHTINGMSEFEAAG
                     VFDVDNDGKLDIVSGNTWYCAPDWKPYPVREVARVGTYFNDFATHPLDINGDGNLDFV
                     TCSYFGKSVGWVENPGKAGAPWTYHEVDVPGNIEASWMVDLSGDGVPDLLPNTVKVVV
                     WYEVVKKEGGNGVELKKHDLGAAAAGHGVGSGDVNGDGRVDLLTPKGWFEAPANPSRD
                     SWAWHPDWQLGATGIQILARDVDGDGLADVVYGMGHDYGLFWLKQTKNSGGDRTWAKQ
                     TIDNSVASVHTLTWADIDGDGKAHELVSGKRVYAHEIEPGATDGSLIAWYAFDPTARS
                     WAKHVIFQGESARNAPAKGADRLALKDFPAGTAGTGLQMTPIDIDRDGDLDLVCPGKS
                     GLYLFENLRLAR"
     gene            complement(2918..>3278)
                     /locus_tag="VHS97_01665"
     CDS             complement(2918..>3278)
                     /locus_tag="VHS97_01665"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446926.1"
                     /translation="DNKGLGIMFQGTDATLLADYSTYKIIAEKGRKIDEPERSLPRSV
                     GHHREWLDAIKSRAQCSCHFGYGHRLASVGNLGNISLWTEEKLTWDPSAERITNHTAA
                     NQYLTKEYRKPWTLPSV"
BASE COUNT          582 a         1003 c          996 g          697 t
ORIGIN      
        1 atctcccccg tcgtctgggt tgcgcttaac accatcagtg gcggcgaggt cctcggtagc
       61 tcggactact agaaaagtgt cacatttgaa ctggagttcc cgaccttttc cccctttttt
      121 caccagtcca tgaccgagcc atcgtcggcg tcataagctt cccggttctt ccagtcgtgt
      181 ccgatctgag cggccatggc gttgtcgtcg agctcgattc caagccctgg acccgtagga
      241 agctcgagat agccgttttt taccacaaac ggttttttca aatagccgtt gcccaggctg
      301 acctgctcct ggcagaagaa gttgggaatt gaagcggcga tctggacacc tgccgcgagc
      361 gagatcggtc ccagtgggtt gtgaggagcg atggccgcgt agtaggcctc agcaattccc
      421 gcgatcagcc ggacctcggt gattccgccg gcgtggcaga ggtcgggctg taagatcgtc
      481 gcggccctct tctccagaac ctcgcgaaat ccccacttgg tgaagacgcg ctcgccggtc
      541 gcgatcggca ggtgcgtgcc ccgcgcgagt tcggccatga tgtcgtggtt ttgcgcctga
      601 caaggctcct cgatgaacat gggctggaaa ggttcgaggg ccttgatcaa gagcttggcg
      661 gtggccgggc tgatcgcgcc gtggaagtcg actgcgatgt cgatatcgtc gcccgccgct
      721 ttacgcagtt cagcgaactt ttcagccgcg taccggacct cagcgggtgt ctcgacatac
      781 cgggccggcc ggcggctggc tggattggtc ttgaaggcgg tgaagccctg cgcaaggccc
      841 ctcttgatac catctacgtt gcgcgcatga gcgtagacgc gcacccgccg ccgggtggga
      901 ccgccaagca gttcgtatac cggtactccc aacgctttgc ctttgatgtc ccacaaggcc
      961 atgtcgacgc cgctgagagc gctggtgaga accgggccgc ctcggtagaa cgcgtggcgg
     1021 taaatcgcct gccaatgatg aaccacctgg cggggatctt tgcccacgag gtaaggctcg
     1081 atctctttca ccgcggtcgc gacggtcgcc gcccgtccct cgacgagcgg ctctcccagc
     1141 ccgacaatcc ccgcgttcgt ataaaccttc aaaaacagcc accgcggctt gacgaacaag
     1201 gtttccagct tcgtgacccg aagaggatcc ttggcggcga tcggagcctc ggtcgaaacg
     1261 gggttttgag ccgttgcctc tacgcggtcg agcgcggcca ggcccagacc cccaagggaa
     1321 gcggcgacga catggcggcg gttgattagt cccgaagcaa tctttcttgt tttcatcgtc
     1381 gtcacttcct cgactttggt ttgaaattga tgtagttcaa tctggtgcaa tcccgtcgca
     1441 atttactacg ccgcgaccct gcgggtcgcg gcgtacaaga cgcacctcgc tccgcgccca
     1501 tcttaactgc ggagaccggg atcccgtatt caggccgatc cgcaagcagg agattcggcg
     1561 attgagggcc atttattccc agaacaaggg cgtgatctac ggggtcgcaa taggtgcgac
     1621 cgtgttggac cgacgtggct accgcgcgag tcgcaagttt tcaaaaaggt agaggccgct
     1681 tttgcccggg cagaccagat cgaggtcgcc gtcgcggtcg atgtcgatgg gcgtcatttg
     1741 caaaccggtg ccggccgttc cggctgggaa atctttgagc gcgaggcggt cggccccctt
     1801 tgcgggtgcg ttccgggccg attccccctg gaagatcacg tgcttggccc aactcctggc
     1861 ggtcgggtcg aacgcgtacc aggcgatcaa cgacccgtcg gtcgcacccg gctcgatctc
     1921 gtgggcatag acgcgcttgc cggacaccaa ctcatgggcc ttgccgtcgc cgtcgatatc
     1981 ggcccatgtg agagtatgga ccgaggcgac tgagttatcg atcgtctgct tggcccaggt
     2041 ccgatcgccc cccgagtttt tcgtctgctt gagccaaaag aggccgtaat cgtgacccat
     2101 gccgtaaacg acgtcggcaa gtccatcgcc gtcgacgtcg cgcgctagaa tctggatgcc
     2161 ggtcgcccca agttgccagt cggggtgcca cgcccacgag tcgcgagaag gattcgccgg
     2221 agcctcgaac cagcccttgg gcgtgagcag gtcgacgcgg ccgtcgccat tgacatctcc
     2281 cgagccgact ccgtgtccgg ccgcggccgc acccaggtca tgcttcttga gctcgacccc
     2341 attaccccct tctttcttca ccacctcgta ccagacgacc actttgacgg tgttgggtaa
     2401 gagatcgggc accccgtcac ccgacagatc gaccatccag gatgcctcga tgtttccggg
     2461 gacgtcgact tcgtgatacg tccagggcgc tccggctttg ccggggtttt cgacccagcc
     2521 gacgctcttg ccgaagtaag agcaggtgac gaagtcgagg ttgccgtcgc cgttgatgtc
     2581 gagcgggtgg gtggcaaagt cgttgaaata cgtgccgacg cgagcgactt cgcgcacagg
     2641 atacggcttc cagtccggtg cgcagtacca ggtgttgccc gaaacgatat cgagcttgcc
     2701 gtcgttgtcg acgtcgaaaa cgcccgctgc ttcgaactcg ctcatgccgt tgatcgtgtg
     2761 ttgcttccag cggggttcat cccccttgat ggatgctgtg tttagcgcga agagtagagc
     2821 cgtgctggcc actgccagtt gcaatcgatt catgatgcct tttccgcctt tcaagatcca
     2881 aatcaagatt caaactcggg aggagatatc gcgccgttca aacactcggc agcgtccagg
     2941 gcttgcggta ttccttggtc aggtattggt tggccgcggt atggttcgtg atccgctcgg
     3001 cgctgggatc ccaggtcagc ttctcctcgg tccagagcga gatattgccc aggttgccga
     3061 ccgacgccag gcggtggccg tatccgaagt ggcacgagca ctgggcgcgc gacttgattg
     3121 cgtccaacca ctcgcggtga tggcccacgg agcggggcag acttcgctcg ggttcgtcga
     3181 ttttccgacc cttttcggca atgatcttgt acgtggaata gtccgcgagc agcgtggcat
     3241 cggtcccctg aaacatgatc ccgagcccct tgttgtcg
//
LOCUS       DATAQL010000100         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4437466, whole genome shotgun sequence.
ACCESSION   DATAQL010000100 DATAQL010000000
VERSION     DATAQL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4437466"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..1566
                     /gene="pdxA"
                     /locus_tag="VHS97_01670"
     CDS             421..1566
                     /gene="pdxA"
                     /locus_tag="VHS97_01670"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX3446927.1"
                     /translation="MTVPEERPLVALTMGDVAGIGPEVIARAWNDPRLHALARPFVIG
                     DPAVLERALKLVGADNRTRVDVVTTPEEADPSLDVIPCLTVVIDGVDLAQVAPGVVDA
                     RAGRAAYEFLIMAARLAIAGRIDAITTLPLNKQALHQSGVRHPGHTEILAKYCGVADH
                     AMMLYLETQGAGTNGSPVPGRRGGTGLGVVHVTLHVALRRIFQLVTTEAVLAKIRLAD
                     QAMRPLTAVWPPRIAVASLNPHAGEDGLFGDEEQRIIQPAVELGRREGFDVTGPFPND
                     TLFYHALAGDYDAVVAIYHDQGHIALKTIGFDRAVNITLGLPIVRTSVAHGTAFDIAW
                     KGLANPASLLESVRVAARIVAGKLRATEVELEGLGQSVLLSRQRSIG"
     gene            complement(1714..2988)
                     /locus_tag="VHS97_01675"
     CDS             complement(1714..2988)
                     /locus_tag="VHS97_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446928.1"
                     /translation="MRQASESNRVGVSIGGELEPGQDPEGSRRPPGRGTETPARRRWL
                     SEPKAVIWLALAAAVLVGGGRRLIAWWQARRAVARLDDPDVTAAEIEAVAEYGRNGVY
                     DLLRIFSSARSEQARQAAGSSLARLWLLDQLVAEEEQAVVRRGFTVTWSARRRYPRSL
                     KTEIPIAVAYEVPFLDDSGRRVDPKNLEWSHRVLGARRITLEEFSQWTPGAGRVAFTI
                     FPSDFETNGPHRLVLEARVRTVDLTDYWEIEPPHVPFLFEFDPILKLDAILTLPDATR
                     DELFARAIRLEPATVADGANSTYVSLGAEWALRDPPRLAVALPLPCDLAHEISIEIQG
                     NAGRLAGGSLVVSGQGLARQGSPATEAVVRRFDLGPIADLAPGAIERPGTRSMRLSLQ
                     AAPDRGWADPDVRSIWPGQLETNWVDVEIVRR"
     gene            complement(3030..3839)
                     /locus_tag="VHS97_01680"
     CDS             complement(3030..3839)
                     /locus_tag="VHS97_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446929.1"
                     /translation="MTRRHWLLLLAGLVFGLLISGRFALTAQDRAETPQAKSSHSPRE
                     KTGESAKLPALSADASDPLLQPLVVQGNQVAVTLQDALLRPYPFPFSRPTSLAEVCAH
                     LKETLKAPVVLDLAALGRKSAKPDDTVQLELDGVRLKTGLKLLLDQVGLTFRVVAEDN
                     LMIITDQEGAEDPAERIWAELRALHRDLHAVQDAVDELADYVGIEGAEGPRVRQPTII
                     EDMPENAGDKPVIIPEKPGRAPEKPGETGAPAPGSRPLAPRVPLARPRRVL"
BASE COUNT          766 a         1376 c         1338 g          721 t
ORIGIN      
        1 cgtcggccag ccaacatccg cgcgccggag ggcgatgtag gtcgggcttt ctagcccgac
       61 gtcggccagc caacatccgc gtgccggagg gcggggtcgg aagacccgcg cccaaacatc
      121 cacccgcgcc caacaaacgc gcgaagttcg gcgagggtct ccgaccccgc cggtacgcca
      181 gaccgaaggt ctacgcgccc acagtccagg cggtgccgct acccacaccg cccacttccc
      241 gcgcctgtga tgccagaatc cgtcgacgca cccggggtgg tgggcgggtc agtattggac
      301 gcgtttcgtg gcgctggcgc acgtgcctag cgctccattc tggccgggct ccgcggtaac
      361 agatacaatc gaagtatcca agcgctcgtt tttggggcat catttcgctc gagaatcgcc
      421 atgacagttc ccgaagaacg ccctctggtg gccctgacga tgggcgacgt ggcgggcatc
      481 ggaccagagg tgatcgcgcg agcctggaat gatccgcggc tgcacgccct ggcccgtccg
      541 ttcgtgatcg gggacccggc cgtgctagaa cgcgctctca agctggtcgg cgctgacaac
      601 cgaacgcgcg tggatgttgt aaccacgccc gaggaggccg acccgagtct ggacgtcatc
      661 ccttgcctca cggtcgtgat tgatggagtc gatctggcgc aagtcgctcc cggtgtcgtc
      721 gatgcacggg ccggccgggc cgcgtatgaa tttctcataa tggcagccag gctggcgatc
      781 gctggccgca tcgacgccat cacgaccctg cccttgaaca agcaggccct ccaccagagc
      841 ggcgtccgcc accccggcca caccgagatc ctcgcgaaat actgcggcgt ggccgaccac
      901 gcgatgatgc tctatctcga aacacaaggt gccggcacaa acgggtcgcc tgtacctggc
      961 cgccgcggcg gtactggcct gggtgtcgtc cacgtgacgt tgcacgtcgc gctgcggcgc
     1021 attttccagc ttgtaacgac ggaagccgtc ctcgccaaga tccggctggc cgaccaggcc
     1081 atgcgcccct tgaccgccgt ctggccgcct cgaatcgccg tggcctcgct caatccgcat
     1141 gcgggtgagg atggtctttt tggcgacgaa gaacagcgga tcatccagcc tgccgtcgag
     1201 ctcgggcggc gagaagggtt cgacgtgacc gggccgtttc ccaacgacac cctcttctat
     1261 catgccctcg cgggcgatta cgacgccgtc gtggcgatct accacgacca aggccacatc
     1321 gcgctcaaga caattggctt cgaccgcgcc gtcaatatca cgctggggct gccgattgtg
     1381 cgcacgagcg tggctcatgg aacggctttc gacatcgctt ggaagggcct ggccaatccg
     1441 gccagcctgc tcgaaagtgt gcgggtcgcc gccaggatcg tggcgggcaa acttcgtgcg
     1501 actgaggtcg agctggaagg cttgggccag tctgttctgc tatcgcgaca gcgatccatc
     1561 ggctagactg acgttcaatc aaaagaagca cggccccaag agcgatgggc tattgcttac
     1621 ccatgacgat catctgggga tggagcgccc caactaaagc ggatccgacc agctgcgctc
     1681 gggcaccgac ccaggctaag ccggctctgt gcgctagcgc ctgacgattt cgacgtcgac
     1741 ccaatttgtc tctagctgac cgggccagat cgaacggaca tccgggtctg cccagccgcg
     1801 gtccggggcc gcttgcagcg agagacgcat cgaccgagta cctggccgct cgatcgctcc
     1861 cggggccaaa tcggcaatcg gccctagatc gaagcggcgg acgacggcct ctgttgcggg
     1921 cgaaccttga cgcgcgagcc cctggccgct cacgaccaag cttccgcccg cgagacgacc
     1981 tgcgtttccc tggatctcga tcgagatctc gtgggccagg tcgcagggca gaggaagtgc
     2041 caccgcgagc ctgggcgggt cgcgcagcgc ccactccgca ccgagcgaga cgtaagtcga
     2101 attcgcgccg tccgccacgg tcgccggctc gagacggatg gcccgcgcga acagctcgtc
     2161 gcgggtcgcg tcgggcaacg tcaggatggc atcgagtttc aagatcggat cgaactcgaa
     2221 gaggaacggc acatgtggag gctcgatctc ccagtaatcc gtcagatcga ccgtgcggac
     2281 gcgggcctca agaaccaggc gatgcgggcc attggtctcg aaatcgctgg ggaaaatcgt
     2341 gaatgcgacg cggccggcgc ctggagtcca ctgggagaac tcttccaggg taatgcggcg
     2401 cgcacccagc acgcgatgcg accattccag gtttttgggg tcgacgcggc ggccgctgtc
     2461 atcgagaaac ggcacttcgt aggccaccgc gatcggaatt tcggttttga gcgatcgagg
     2521 gtagcggcgg cgggcgctcc aggtcacggt gaacccgcga cgcacgacgg cttgctcttc
     2581 ctcggcgaca agctgatcca aaagccagag ccgcgccagc gacgaaccgg cagcctggcg
     2641 agcttgttcc gatcgcgccg agctgaagat ccgcagcaga tcgtaaacgc cgttcctgcc
     2701 atattcggcg acggcttcga tctccgccgc cgtgacgtcg gggtcgtcca gccgggcaac
     2761 cgccctccgc gcctgccacc acgcaatcag ccggcgtccg ccgcctacga gtacggcggc
     2821 ggccaacgcg agccagatca ccgccttcgg ctcactcagc cagcggcgcc gggcgggggt
     2881 ttccgtacca cgcccaggag ggcggcgcga cccctcagga tcctgacctg gttccagctc
     2941 accgccaatt gaaaccccga cccgattcga ctcgctcgcc tgccgcatcg atgctctctg
     3001 aatttaacgg tattggcact aagccggcgt catagaacgc ggcgcggacg ggccagggga
     3061 acgcggggtg ccagcgggcg cgagcctggc gctggcgctc cagtctcgcc gggcttttcc
     3121 ggcgcacgac ccggtttttc aggaatgatc accggtttgt cgccagcgtt ctcgggcatg
     3181 tcttcgatga tggtcggctg gcgcactcga ggcccttcgg ctccttcgat cccgacgtaa
     3241 tcggctagtt cgtcgacggc gtcctgcacg gcgtgcaggt cgcgatgtaa agcgcgcaac
     3301 tcggcccaga tccgctccgc gggatcctcc gcgccctctt ggtcggtgat gatcatcaag
     3361 ttgtcctcgg cgaccacgcg aaaagtcaaa ccgacctggt cgagcaatag cttcaggccg
     3421 gtcttcaagc gaacgccgtc cagctcgagc tgaacggtgt cgtcgggctt ggcgctcttg
     3481 cgtccgaggg cggcgaggtc gagcaccacc ggggccttaa gtgtttcttt caagtgagcg
     3541 cagacctcgg cgagcgaggt tgggcgcgag aacggaaagg gatagggccg caagagcgca
     3601 tcctgaagcg tgaccgcgac ctggttaccc tggacgacga gcggctgcaa gagcggatcg
     3661 ctcgcatcgg ccgacagtgc cggcaatttg gcggattcgc cggttttctc cctgggtgag
     3721 tggctgcttt ttgcttgtgg ggtctcggcc cggtcctggg ccgtgagcgc gaaccgcccc
     3781 gagatcaaga gcccgaaaac caggcctgcc agcagcagta accagtgtct tcgcgtcatg
     3841 caccgctcct cttagacacg aggcgcgagg cggaatgggc gcctgcaggc gcaaagtcgg
     3901 cgatcattcc ccttttctgg aacattatgg cgcattgtaa ccgcgacgtc tcggaccaac
     3961 cagcgggact tgcggccgaa acgttgacct cgatcagctc aaccacgcct aatctttcac
     4021 agggaggcac tcgagcaggc ggtcaagtag aatggtcgtt cgagaccatg gcaaacgaac
     4081 aagtgtaggc acgaactggt gctacgtgag ctgtgaccca caatgggtgg gcttggcggc
     4141 ttcctcacgc aggcaacatc atgaacagtc cacaaccgct gggccccaga ctcgaactta
     4201 t
//
LOCUS       DATAQL010000101         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4437575, whole genome shotgun sequence.
ACCESSION   DATAQL010000101 DATAQL010000000
VERSION     DATAQL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4437575"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VHS97_01685"
     CDS             <1..248
                     /locus_tag="VHS97_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3446930.1"
                     /translation="RDHWPLCFTVGFAGGGVQGGRVVGASDPIGAVPADRPVEPPDIA
                     ATIFHSLGLNLETKLPGPAGRPFPLVDVGHREIQELF"
     gene            271..>1967
                     /locus_tag="VHS97_01690"
     CDS             271..>1967
                     /locus_tag="VHS97_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX3446931.1"
                     /translation="MRIRLVFVVAGIIGYWFAPAADALAADGFVLFPSECVLSTPASR
                     QQLIAQEIEHGEVGRQRVAGIAWSSSDPKIATVVDGIVTPVHDGQATITAEVGAHKAT
                     AKVVVAGMSLPFAWSFRNHVEPILAKQGCNSGACHGALAGKGGFRLSLQGYDPAADFF
                     NMVKQDRGRRVELADPGQSLLVAKPSGAIAHKGGVRFATSALEYRILSEWISNGAAPP
                     SEADPRVEELEILPARSFQRVGESQQILVRARYSGGQAEDVTRWVKWSSADESVCRVD
                     EQGKTQVIGPGEGAIVAWYASKLAIARVTVPYEGQQSQAAATALHPKPRNFVDEQIDK
                     QLARLNLAPSPLCTDAEFVRRAYVDTIGRLPSVDEVRAFLGDASPSRRDALIESLLAR
                     PEFSDYWTYKWSDLLMLNGTRLRPAALKTYYQWIHKQVADGVPWDQFVRAIITSTGES
                     VENGPTNFYALSQSPEDMTENVSQAFLGLSIGCAKCHNHPLEKWTNDQYYGMASMFAR
                     VRAKGWGGEGRDGDGRRTLYVAESGELVQPRTGKPQPPAPLDGHALKFDDPADRRVSL
                     "
BASE COUNT          371 a          629 c          636 g          331 t
ORIGIN      
        1 gccgcgacca ctggccgctc tgcttcacgg tcggctttgc cggcggcggt gttcaagggg
       61 gacgtgtggt gggtgccagc gatccaatag gcgccgtccc ggcggaccgt ccggtcgagc
      121 cacccgacat cgccgcgacg atcttccaca gcctcggtct caacctcgaa acgaagctcc
      181 cgggtccggc ggggcggccg tttccgctgg tcgatgtcgg acatcgagaa atccaggaac
      241 tcttctgatt gccactacga ggtaatgtgc atgaggatcc gactcgtttt cgtcgttgct
      301 gggatcatcg gctactggtt tgcaccggcg gctgacgccc ttgcggctga tggtttcgtc
      361 ctgttcccgt cggagtgcgt gctcagcacc ccggcgagcc ggcagcaatt gatcgcgcaa
      421 gagatcgagc acggcgaggt aggacgtcag cgggttgccg gaatcgcgtg gtcgtcaagc
      481 gaccccaaaa tcgccaccgt cgtcgacggt atcgtgaccc cggtccacga cggccaggcc
      541 acgatcaccg cggaagtggg tgctcacaaa gcgacagcca aggttgtggt cgcaggcatg
      601 agtctcccgt ttgcctggag tttccgcaat cacgtcgagc cgatattggc gaagcaaggg
      661 tgcaactcgg gtgcctgcca cggtgccctg gcaggaaaag ggggcttccg gctctcgctc
      721 cagggctatg atccggcggc cgacttcttc aatatggtga agcaagaccg aggccgtcgc
      781 gtggagctcg ccgatccggg gcagagcctg ctcgtagcga agccttccgg cgcgatcgcg
      841 cacaaagggg gcgtgcggtt tgcgaccagc gcgctcgaat accgaatctt gagcgagtgg
      901 atcagcaacg gcgcggcacc tcccagcgag gccgacccac gggtcgaaga attggagatc
      961 ctgccggccc ggtcgtttca gcgcgtcggt gagagccagc agatcctggt ccgtgcccgt
     1021 tattcgggcg gccaggcaga agacgtcacc cgctgggtca aatggtcgtc cgccgacgag
     1081 tcggtctgcc gcgtcgatga gcaagggaag acgcaggtga tcgggcccgg cgagggagcg
     1141 atcgtggcct ggtacgcaag caagctggca atcgcccggg tcaccgttcc atacgaaggg
     1201 cagcaatccc aggcggcagc gaccgcccta caccctaagc cccggaactt cgtcgacgaa
     1261 cagatcgaca aacaactggc gcggctcaac cttgcccctt cgccgttgtg caccgacgcg
     1321 gaattcgtcc gccgcgcgta cgtcgacacg atcggccggc tgccttcagt cgacgaagtc
     1381 cgggcctttc tcggcgacgc ttcaccgtcc cggcgcgacg cactcatcga aagtctgctc
     1441 gcccggccgg agttcagcga ctactggacc tacaaatggt ccgacctgct gatgttaaac
     1501 ggcacgcgac tgcgcccggc agcgctcaag acctattatc agtggatcca caagcaagtc
     1561 gccgacggcg tgccgtggga ccagttcgtg cgcgcgatca tcacttccac cggtgaaagc
     1621 gtcgagaacg gccccaccaa cttctacgcg ctcagccagt cgcccgaaga tatgaccgaa
     1681 aatgtcagcc aggcgttcct gggactgtcg atcggctgcg ccaagtgcca caaccaccca
     1741 ctcgaaaaat ggacgaacga ccaatattac ggtatggcca gcatgttcgc acgggtcagg
     1801 gccaagggct ggggcggtga ggggcgagat ggagacgggc ggcgcaccct ctatgtggcg
     1861 gagtcgggtg aactggtcca gccccgaacg ggcaagcctc agccgccggc gccgctcgac
     1921 ggacacgcgc tcaagtttga cgatcccgcc gatcgccggg tgagcct
//
LOCUS       DATAQL010000102         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1689900, whole genome shotgun sequence.
ACCESSION   DATAQL010000102 DATAQL010000000
VERSION     DATAQL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689900"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1982)
                     /locus_tag="VHS97_01695"
     CDS             complement(87..1982)
                     /locus_tag="VHS97_01695"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446932.1"
                     /translation="MVLYASTHAHETIHVLWNMGAIGGLTDAQLLERFLSGPDEGSEV
                     AFTVLVERYGPNVRRVCLDMLSDPHEAQDAAQAVFLVLARKARSIRKPESLGPWLHGV
                     AVRMARRIRAEAARRRKIEQRRAEAMAERFHTQHNAEPDRYIELYEEIERLPEKYRNP
                     IVLCYLQGHTQEQASQRLGWPLGTVQTRLHRGRERLRTRLSRRGIGPSAIAATAVALP
                     RNTATAAAVPVPPSWVNATARAAVHFAAGTPTAGHVAQVVVRLAEGALVTMLRETLKA
                     FALGILVVGLASFGVALGVRAVTNGREPDRRSATPTAAPAQPVVARAQPVRATPPPRK
                     RPNVDLTIKAGARYLEARQQRDGSWPDVDIEAHTGLTSLVTLALLGADEPAALPSITR
                     AIEYLRGFTPDQLDSVYAVSLQTRAFAAMDVEQDVPRIARNVEWLERAQIKPGDRVEW
                     PGSWTYNQAKNRSGDNSNTFYALLGLAAAAEAGVPVKAEVWTLADRYWSSSQRPDGGW
                     GYTPLANSATSSMTCGALASLILTRTRLNEDQESPDGTGQPGGVQPAKAAPRLLPRRR
                     PPAHGASRPGAIGGAHRAAQSTGMVATETGRSHPGHCREARQLGAAGVGPIAEDARAT
                     SLDSLGR"
BASE COUNT          362 a          687 c          645 g          419 t
ORIGIN      
        1 tggactcact gatgagctcg gccggatact ttacgacgcc acgatgcaac gaggccgcca
       61 gcctccagag tcgcgtcagc tccgactcac cggcccagag aatccaggga tgttgctcgg
      121 gcgtcctccg cgatcggacc cacccccgcc gcgcccagtt ggcgagcttc ccgacagtga
      181 ccgggatggc ttcggccagt ctcggtagcc accattcctg tggactgagc tgctctgtgt
      241 gcgcctccga tcgcgccagg ccgcgacgca ccatgagctg gcggacgacg tcggggaaga
      301 agccgcggcg ccgctttggc gggctgaacc cctcccggtt gcccagttcc atctggagat
      361 tcttggtctt cgttcaggcg ggtccgggtc aagatcaagc tcgcaagcgc tccgcaagtc
      421 atgctggagg tcgccgaatt cgccaaaggt gtgtagcccc aaccgccatc ggggcgttgt
      481 gagctgctcc aatagcggtc ggcgagcgtc cagacctcgg ccttgactgg aacgccggcc
      541 tcggctgccg cggctaatcc gagcagggca tagaacgtat ttgagttgtc gccgctccta
      601 ttcttggctt gattatatgt ccagcttccg ggccattcga cacgatcgcc aggcttaatc
      661 tgagcccgtt cgagccattc cacgttgcgg gcgattcgcg gcacgtcttg ttcgacgtcc
      721 atcgctgcaa acgccctcgt ttggagcgaa accgcgtaga ccgaatccag ttgatcgggc
      781 gtaaagcctc tgagatactc aatcgcccgc gttatggagg gtaaggcggc aggttcgtcg
      841 gcccccagca aggcgagcgt gaccaggctt gtcagtcctg tatgcgcctc gatatcgacg
      901 tcgggccatg agccatcccg ctgttgccgc gcttcgaggt agcgcgcgcc tgccttgatc
      961 gtcaggtcga cattgggccg ctttcggggg ggtggcgtgg cccggactgg ttgcgcacgc
     1021 gccaccacgg gctgggccgg cgcggcggtt ggtgtcgccg atcgcctgtc tggttcacgg
     1081 ccgttcgtga ccgcacgaac gcccagcgcc acaccaaagc ttgccaggcc cacaaccagg
     1141 atgccgagcg caaaagcctt cagcgtctcc ctcagcatcg tgacaagggc gccctcagcc
     1201 aaccgcacca caacctgggc aacgtgccca gccgtcggtg tgccggcggc aaagtggacg
     1261 gctgcccttg cggtcgcatt tacccagctg ggcgggaccg gaaccgcagc cgcggtcgcg
     1321 gtgttccgtg gtagtgccac cgctgtcgcc gcgatagcgc ttggcccaat gccacgccgg
     1381 gagagccgag tacgtagccg ctcgcggccg cggtgaaggc gtgtctgaac ggttcccagc
     1441 ggccatccga gccgctgtga agcctgctcc tgggtgtggc cttgaagata gcagaggacg
     1501 atcgggtttc gatacttctc gggcagccgc tcgatttcct catagagttc aatgtaccgg
     1561 tctggctcag cgttgtgctg cgtgtggaat cgctcagcca tggcctcagc ccttcgctgt
     1621 tcgatttttc gtcttcgagc agcctcggcc cgaatccgcc gcgccatacg cactgccacc
     1681 ccgtgcagcc acgggccgag cgactcgggc ttgcggatcg atcgagcctt cctcgccagc
     1741 acaaggaaaa ccgcctgggc cgcgtcttgt gcctcatggg gatcgctgag catgtcgagg
     1801 cagacgcgtc gcacgttcgg cccgtaccgc tcgacgagta cggtgaaagc aacctctgat
     1861 ccctcatcag gcccggacag aaatcgctcc agcaattgcg cgtcggtcaa acccccgatc
     1921 gcccccatat tccagagcac atggattgtt tcatgtgcat gtgtcgatgc gtagagcacc
     1981 atcggctcga aactcctgac gcaatcggaa cgcgcgtttt ctcccatagt gacgtcctgt
     2041 gccggctttc atttcagaat tccgggacgt cgcgaatttt tgtaaactta tccagtgatg
     2101 gaatctggat tta
//
LOCUS       DATAQL010000103         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1384095, whole genome shotgun sequence.
ACCESSION   DATAQL010000103 DATAQL010000000
VERSION     DATAQL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384095"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VHS97_01700"
     CDS             complement(<1..367)
                     /locus_tag="VHS97_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEX3446933.1"
                     /translation="MATAISSHTRGSGTPDNLVDRRVAAFCSTRYPELFHTVAYANDI
                     WKHDPFDVESIHRNAREKFWRIVGRVLEPSGLASGRILLLLGESGCGKTHLMRAFRNQ
                     IHSQSSGYCGYLQMTAFTGH"
     gene            complement(380..1453)
                     /locus_tag="VHS97_01705"
     CDS             complement(380..1453)
                     /locus_tag="VHS97_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446934.1"
                     /translation="MTTPAPRNLSPIESLVLVRLLATGEKGEASAKIKKDLEPLLAHR
                     WQGAALTERIEQTLSALESRGLLALVPGKTKKAAPKFVRTAEGHRNGLEFLGVSQLRS
                     KTTWAVLRKTYLPACAIGIPASSEARFKALSSEPAFQAVLLRQQYGLSIAELPKPDQA
                     TDGLAWKLIGFEGEDRKFNPKNVKTALFNRALGDGRATDFKKAATRLLAQRVGARRDD
                     PKELRDAIVRGLIDQEATSANPAESEDVLPQSSPMSSALARSPVDLRTFAEQVKSAAR
                     DCRTGRFGDNKVFIAHVWKALQVDPAFRSMDLAAFKEHLAEANNVRLLDLSRADLVQA
                     MDPDDVRESEVHYMSATFHFVRI"
     gene            complement(1450..>2543)
                     /locus_tag="VHS97_01710"
     CDS             complement(1450..>2543)
                     /locus_tag="VHS97_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX3446935.1"
                     /translation="LVGVRLKEADPEAVVVPWVDELANKHDLGTAAVHWSAQLLSRNA
                     QALERSLRLMTTHERGMCLESCLPLESGTSLALLGRCLIEFAATGTRPGGPDFARELN
                     IPLQGYGRPWIRIFRAIGDFVNWRCLPVLVVSLAKPSVTGLEGIARLLGELATGQPRG
                     AMALIVSQDLFDSFVALTPASRAKALLLESIVTLNGKQSSVTAEPVLPSLLAPESTCD
                     DLIEDDLESRIRDLEAPQQSSDDDEARSAAERCLFERLESGAETAGLFELNATLDFYF
                     GSSRWIEVDFAARSLKLAIEVDGYHHFHDPEAFRRDRRKDLELQKHGYLVVRVLAEDV
                     VERLEEVLDTILEAVAFRRAAAIHLGATS"
BASE COUNT          522 a          773 c          756 g          492 t
ORIGIN      
        1 agtggccggt aaacgccgtc atctgcaggt agccgcaata tcccgagctc tgggagtgga
       61 tctggttccg aaaagcacgc atcaagtggg tcttgccgca gccggattcc cccaatagca
      121 ggagaatcct gccgctggcc aggcctgatg gctcgagcac tcgtcccacg atccgccaaa
      181 acttctcgcg ggcgttacgg tgaatcgact ccacatcgaa ggggtcgtgt ttccagatgt
      241 cgttggcgta ggcgacggta tggaaaagct ctggataccg ggtcgagcag aacgcagcga
      301 cgcggcgatc gaccaggtta tccggggtgc cagagccgcg tgtgtggctc gagatggctg
      361 tcgccataag ggtgcccttt cagatccgta cgaaatgaaa cgtggcgctc atatagtgta
      421 cctcggactc acgaacatcg tcgggatcca tggcctgaac caggtcggcc ctgctgaggt
      481 cgagcaacct cacattattc gcctcagcaa ggtgttcctt gaaagcggct agatccatac
      541 tccgaaacgc gggatcgact tggagagcct tccagacatg ggcgatgaac accttattgt
      601 caccaaaccg gccggtgcga cagtcgcgtg cagcggactt cacctgctcg gcaaaagtcc
      661 gtaaatcgac cggcgaccgg gcgagggcgg aggacatcgg cgaggattga ggcaagacgt
      721 cctctgattc agcggggtta gcgctagtag cctcctgatc gatcaaaccg cgcacaatcg
      781 cgtcacgcag ctctttggga tcgtcgcgcc gcgcgcccac gcgctgcgcc agcaggcgag
      841 tggcggcctt tttgaagtcg gtcgcacggc catcgccgag ggcgcggttg aatagcgctg
      901 tcttgacgtt cttggggttg aatttccgat cctcgccttc aaagccgatc agcttccagg
      961 ccagaccgtc ggtggcttga tccggcttgg gcagctcagc aatcgagaga ccgtactgct
     1021 gcctcagcag gacggcttgg aaagccggct cggaagagag cgccttgaac cgggcctcgc
     1081 tcgatgcagg tatgccgatc gcgcatgcag gcagataggt cttcctgagc acggcccaag
     1141 tggtctttga cctgagctgc gagacaccca ggaattcgag accgttccga tggccttcgg
     1201 cggtgcgtac aaacttgggc gcggccttct tggtcttacc ggggacgagc gccagcaaac
     1261 ccctggactc gagagcgcta agcgtctgtt cgatccgctc ggtcagtgcc gcgccctgcc
     1321 atcgatgcgc gagcagcggc tccaggtctt tcttgatctt cgcgcttgcc tctccttttt
     1381 cacccgtggc cagtagccgc accaggacaa gcgactcgat cggcgataga ttcctgggtg
     1441 cgggagtcgt catgatgttg cccccaggtg gatcgccgct gcgcggcgaa acgcaacggc
     1501 ctcaaggatt gtgtccaaca cttcctcgag ccgctccacc acgtcttcgg ccaacacccg
     1561 cacgaccagg tagccgtgct tttgaagctc caggtctttg cggcgatcgc gacgaaacgc
     1621 ttccggatcg tggaaatgat ggtaaccatc gacctcgatg gcgagcttca aagagcgggc
     1681 agcgaagtcg acttcgatcc aacggctgga tccgaagtag aagtcgagcg tcgcattgag
     1741 ctcgaacaac ccagcggtct cagcaccgga ttccagccgc tcgaacaaac accgttccgc
     1801 ggcgctgcgg gcttcatcgt cgtcggaact ttgttgcggc gcttcgagat cgcgaattct
     1861 cgactccaga tcgtcttcaa tcaaatcatc gcatgtagat tcgggagcta agagggatgg
     1921 taacacaggc tcggcggtga ccgacgattg ctttccgttc aaggtcacga tcgattcgag
     1981 aagcagggcc ttagcgcgcg aagccggtgt gagtgcaacg aagctatcaa acagatcctg
     2041 actgactata agggccatcg ccccgcgcgg ctggcccgta gccaactcgc ccagcagccg
     2101 agcaattcct tccagcccgg tcacactggg cttggcgaga ctcacgacca agactggtag
     2161 gcacctccaa ttaacaaagt cgccgatggc cctgaagatg cggatccacg ggcggccata
     2221 gccctgtagc ggtatattta gctcgcgcgc gaaatccggg cctcccggac gtgttcccgt
     2281 cgccgcgaat tcaatcaaac agcggcctaa aagcgcaagg ctggtcccgc tttcgagcgg
     2341 taaacaggat tccaggcaca ttccgcgttc gtgagtcgtc atcaggcgga gcgaccgctc
     2401 gagcgcttgg gcatttcgac tcaacagctg cgctgaccag tggacggcgg cagtccccaa
     2461 gtcgtgctta ttcgcgagtt catcgaccca cggaactaca acagcctcag gatcggcctc
     2521 tttcagccgc acgcccacca atg
//
LOCUS       DATAQL010000104         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3216697, whole genome shotgun sequence.
ACCESSION   DATAQL010000104 DATAQL010000000
VERSION     DATAQL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3216697"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VHS97_01715"
     CDS             complement(<1..958)
                     /locus_tag="VHS97_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3446936.1"
                     /translation="MDDLFGPESRRDLIRRGFSRRDFGRLAAFLTAGAALPFYNEAAL
                     AQGLSALPNLAPDAVRINANENPMGPCPEAAEAIHKVVSQGGRYLYNETGAFVAAMAE
                     VEGVSKDHVMPFAGSSDPLHRAVLAFTGPTKSYVVAEPGYEAGARGAEFIGAKTVRVP
                     LRKDYAHDVHAMAQADPNAGLIYVCNPNNPTGSITSKEDIEYLVAHKPKDAIVLLDEA
                     YIHLSKNAEPASALVAADKDVIILRTFSKLYGMAGLRAGAALGRPDLIAKLRGYGAGA
                     LPVTGMVGATVSLKVKGLVAERRKTIADIREETAEWLDKRGFG"
     gene            complement(1273..1632)
                     /locus_tag="VHS97_01720"
     CDS             complement(1273..1632)
                     /locus_tag="VHS97_01720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX3446937.1"
                     /translation="MSMIRIPRRGEIWLVDFEPSFGAEIRKVRPAVVLSLDQLARLPL
                     RVVVPLTDWKPQYNQYSWFVRIPLSPANGLSKDSGADAFQVKSVSETRFSRFLGAASP
                     IELDAIATAVAFVVGKP"
     gene            complement(1601..1912)
                     /locus_tag="VHS97_01725"
     CDS             complement(1601..1912)
                     /locus_tag="VHS97_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446938.1"
                     /translation="MPANVSEKLILDALHKVPQAHWSHVLEFLHDLEPKAPLPGEGEM
                     PKTPTIAELLALSSDRRDMILEAQAALAEHDYRNDPELTAFEAFGPDDLYVDDTDTPA
                     R"
     gene            complement(1946..2917)
                     /gene="trpS"
                     /locus_tag="VHS97_01730"
     CDS             complement(1946..2917)
                     /gene="trpS"
                     /locus_tag="VHS97_01730"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX3446939.1"
                     /translation="MRVLSGIQPSGALHIGNYFGALKQFIDLQRDNDAYYFVADFHAL
                     TSVRDAALLRGYTFDVFVTMLSLGLDPEKATLFVQSDVPETTELAWLLSTVTPMGWLE
                     KCVSFKDKIQQGLPAEHGLFAYPVLQAADILLYDADLVPVGQDQKQHLEITRDVAERF
                     NRTYGGGEEIFRLPKPHILEAVAVVPGVDGRKMSKSYNNTIDIFDDPATIRKKVKKIV
                     TDSTPVEAPKNPDTCPLFNLYKLFAAPDERALVERRYREGGIGYGEVKTRLAESIIAR
                     FGPARDLRAEWLAHPERVAAVRAAAADRAKATARRVLDRARAACGVG"
BASE COUNT          564 a          874 c          939 g          598 t
ORIGIN      
        1 cgccgaagcc ccgtttatcc agccattccg cggtctcttc gcgaatatcg gcgatagttt
       61 tgcggcgctc ggcaaccaaa cccttgacct tcaagctcac ggtggcgccg accatgccgg
      121 tgaccggtag cgctccggcg ccgtagcccc gcaatttggc gatcaggtcg ggccggccga
      181 gcgcggcgcc ggcgcggagg ccagccatgc cgtagagctt ggaaaacgtc cgcagaatga
      241 tcacgtcttt gtcggccgcc accagggccg aggccggctc ggcattcttc gacagatgaa
      301 tgtatgcctc gtccagcagg acaatggcat ccttgggctt gtgggcgacc aggtattcga
      361 tgtcttcctt ggaagtgatc gaaccggtcg ggttgttagg attgcagacg tagatcaagc
      421 cggcgttggg gtcagcctgt gccatagcgt gcacgtcgtg ggcgtagtct ttgcggagcg
      481 gaaccctgac ggtcttggcg ccaatgaatt ccgccccgcg cgccccggcc tcatagcccg
      541 gttccgcgac cacgtagctc ttcgtaggac cggtgaatgc caagacggcg cgatgcagtg
      601 ggtcactcga gccggcaaag ggcatcacat gatcctttga cactccttcg acttcggcca
      661 tcgcggcgac gaatgcaccg gtttcgttgt acaggtagcg gcctccctgg ctgacgacct
      721 tgtggatcgc ctcggccgcc tcggggcagg ggcccatggg gttttcgttg gcgttgatcc
      781 gcacggcgtc gggcgcgagg ttgggcagcg ccgaaagccc ctgtgccagc gctgcctcgt
      841 tgtaaaacgg caaggccgca ccggcggtga gaaatgccgc cagccggccg aaatcgcggc
      901 ggctgaatcc gcgacgaatc aggtcccgac gtgactccgg gccgaaaagg tcgtccatcg
      961 ttcctccagg atctgcgtga gtgtcgaatg cagggatcaa cgtgaaacgt gccggacagc
     1021 cgcggtacgg cctggacccg tgcgccgggg ccagtgttga aacggttgtc ggagttcggc
     1081 cgagagggag aagagtcagg acagattaag gagttctcga tctaccctgt ggggcgctca
     1141 gccgcgactt agggcatcat tatagacttg gtcaggtatc tcgtaaacgg gcttgtggca
     1201 cgtgatcggc ccgatgcatt ggccgaacag gattatgctg gatcgcagct tcttttggtg
     1261 ccgtcctgca gttcatggtt tacccacaac gaaggccacg gcagtcgcga tcgcatcaag
     1321 ttcaatcggg ctggctgcac ctagaaagcg agagaaccgt gtttcggaca ccgatttgac
     1381 ttggaacgcg tcggctccag aatccttgct caggccgttt gctggactca gtgggatgcg
     1441 tacaaaccat gaatactggt tgtattgcgg cttccagtcg gtgagcggga caacgacgcg
     1501 gagcggtagg cgagcgagtt gatcgaggct cagcacgacg gcgggtcgaa ccttgcgaat
     1561 ttccgcaccg aaggagggct caaaatccac gagccagatc tcaccgcgcc ggggtatccg
     1621 tatcatcgac atagaggtca tccgggccga aggcctcgaa ggcggtaagt tcaggatcat
     1681 tgcggtagtc atgttcggcg agcgcggctt gtgcctcgag tatcatgtcg cgccggtcgg
     1741 aagagagggc gagcagttcc gcgatcgtcg gcgtctttgg catttcccct tcccccggta
     1801 gcggggcttt cggttcgagg tcgtgaagaa attcaagcac atgactccaa tgcgcttgtg
     1861 gaaccttgtg caacgcgtcg agaatcaact tttctgaaac gttcgcgggc acggaacacc
     1921 tccaggtcct agtgattgtt caatttcatc ccaccccgca agcggcgcgg gcgcggtcaa
     1981 gcacacgtcg ggcagtggct ttcgcacggt cggcggccgc cgcgcggact gcggcgacgc
     2041 gctcgggatg cgccagccat tcggcccgca gatctctcgc cggtccgaag cgggcgatga
     2101 tcgactcagc gagtcgggtc ttcacttcgc catagccgat acccccctcg cggtagcgcc
     2161 gctcgactag ggcccgctca tcgggtgccg caaagagctt gtaaaggttg aagagggggc
     2221 aggtgtcggg attcttgggt gcttccacgg gtgtgctgtc ggtaacaatc ttcttcactt
     2281 tcttgcggat cgtggccggg tcgtcaaaga tatcaatcgt attgttgtag ctcttagaca
     2341 tcttgcgacc gtcgacaccc ggcacaaccg ccaccgcctc caggatgtgc ggtttgggca
     2401 gccgaaagat ctcttctccg ccgccgtacg ttcggttgaa ccgctcggcg acgtcgcgcg
     2461 tgatctccag gtgttgcttc tggtcttggc cgaccggaac caagtcagcg tcgtatagca
     2521 agatgtcggc cgcctgcagc accggatagg caaagagtcc gtgctcggcg gggagtcctt
     2581 gctggatctt gtccttgaag ctgacgcact tttccagcca acccatgggt gtcaccgtcg
     2641 agagcagcca cgccagctcg gtagtttccg gcacgtccga ctgcacgaat agagtcgctt
     2701 tctccggatc gagcccgagc gacaacatgg tgacgaacac gtcgaaggtg tagccgcgca
     2761 gcagtgccgc atcccgaacg ctcgtgagcg cgtgaaagtc ggccacgaag tagtaggcgt
     2821 cgttgtcgcg ctgtagatcg ataaactgct tcaaagcacc gaagtagttg ccgatgtgca
     2881 gagcgcccga gggctggatt cccgacaaaa cacgcatgat gcgatcaact catccggatc
     2941 agtcatcgag ccgacgatgc tcaccaagca ccttc
//
LOCUS       DATAQL010000105         5132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2300015, whole genome shotgun sequence.
ACCESSION   DATAQL010000105 DATAQL010000000
VERSION     DATAQL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300015"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VHS97_01735"
     CDS             complement(<1..401)
                     /locus_tag="VHS97_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX3446940.1"
                     /translation="MRAEIIAIGSELVSGQSLDTNSYWLSQQLTALGIAVAFHTTLGD
                     VLDDHLVAFKNAVTRADVVVMTGGLGPTQDDLTREALARCAGVPLVEDAESLAAIAAM
                     FARRNRVMSERNRVQALFPIGATPLPNPVGTA"
     gene            633..1250
                     /locus_tag="VHS97_01740"
     CDS             633..1250
                     /locus_tag="VHS97_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX3446941.1"
                     /translation="MSGCVLQRSTLVLNRHWQPVHVAPVHRALVMLWNEAACVIDPDD
                     FQLYTWSDWAKLAPREGEPFIRTVRFHLRVPEVLALTRHDKARHQTVTFSRRNLFKRD
                     HSTCQYCGCQPGTEELTIDHVIPRAQGGQTTWENCALACVPCNARKANRTPDQAAMKL
                     RRAPVRPAWKPLYDASGARIASWSRFLSDAYWNVPLQNSGYKIQR"
     gene            complement(1293..2669)
                     /locus_tag="VHS97_01745"
     CDS             complement(1293..2669)
                     /locus_tag="VHS97_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446942.1"
                     /translation="MIRSSTRRRFLEMTGAAAGIAGLTGAVGSAPLGGPNSQPAAARG
                     RRLKVAALATVYNYLSHAYHIVGRFIDGFPVYDGHGLHKPEFEIASLFIEQTPVATDL
                     GRAKARLHGIRQSPSIADALTLGTGKLGVDAVLLIAEHGEYPYNEKLQKLYPRGRFFG
                     EVLEVFKSSGAAVPVFIDKHLSYSRAEAQQMVDQAKSLKVPLMAGSSLPVTWRLPALE
                     IPLGREFKDVVVASRGDLEIFGFHALETLQCMVERRERRGKPQGVATVTCLEGDAVWQ
                     AGDKGEWSWSVLEHALSRSHSVNPGDIKQNTRDFKPPPAPGRDVPTKLGYPVAYIVEY
                     VDGLRATVLILNGHVDDTTIAARLSGSDGKDTIVSTLMYLPAPPGASFFNPLVLRIED
                     FFRTGLSPYPVERTLLTGGVLDAALESRLNDHRPIATPDLAGIDYRAPADSGFIRTPL
                     TNPEPNRL"
     gene            complement(2876..3820)
                     /locus_tag="VHS97_01750"
     CDS             complement(2876..3820)
                     /locus_tag="VHS97_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3446943.1"
                     /translation="MILIPNFVQEGRAEEAQRLIELRWQSLDAHGEVTVEQAVNLARL
                     HMEMRWNIPPAATLRDYLDQVGRLAPDDDRIWLGRANLAIRAGSYDEGRRLLDDCLRR
                     RPDDRAVWRARLDWSMRTNRLDEAKAALKHLPAELATPAKVHCLSAWIASMCGDIDRE
                     RQELAALVAEVPEDFRALERLEKLEQQAPANTALAKPRRPRAEIERDQMRYRDLYRRN
                     QPARDSEEMARLAERLGHRFEAILFLTAAIAEEPDRADLRENLHRLMEAARKPDDRSQ
                     NRFDSVPWDCGGDNSSAAVCTERQHFASDWAGWPFVNS"
     gene            complement(3900..4136)
                     /locus_tag="VHS97_01755"
     CDS             complement(3900..4136)
                     /locus_tag="VHS97_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446944.1"
                     /translation="MLLAINGLLAVLFVVTQLEPNQVAMLGPPGQYLHELFNPPAVPE
                     TSPFGKRLIAGLGHHTAGLYLQRTRDRGPHRPAH"
     gene            complement(4845..>5132)
                     /locus_tag="VHS97_01760"
     CDS             complement(4845..>5132)
                     /locus_tag="VHS97_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446945.1"
                     /translation="WGYLWGLIGLVLAMPITVSLKVVLQTIPELNRWAELMSLDWQSP
                     APAIAAVAGSEQPADPAAPNDPGQANTIIMTSQEFETARAGIENRSASSVS"
BASE COUNT          984 a         1599 c         1558 g          991 t
ORIGIN      
        1 gcggtcccga ccgggttggg caggggcgtg gcccctatgg ggaagagggc ctgaacgcgg
       61 ttgcgttcgc tcatcacgcg attgcggcga gcgaacatgg ccgcgatcgc cgccaacgat
      121 tccgcatctt cgaccagtgg aactccggcg caccgagcga gtgcttcgcg ggtcaggtca
      181 tcctgcgtgg gtccaagacc gccggtcatc acgaccacgt ctgccctggt aacagcgttc
      241 ttgaaggcca ccaggtggtc gtccagcacg tctcctagcg tcgtgtggaa cgcgacggcg
      301 atcccgagcg ccgtcagttg ctggctgagc caatagctgt tggtatcgag cgattgcccg
      361 ctgacaagct cgctgccgat cgcgatgatt tcggcccgca tcggtggttc ccaatcagtc
      421 caagccgggc tacctgcccg gtacgaaatc gataatcaca gtccgaggcc acatcgtgcc
      481 gaccggagaa agtcaatgta gcacccccaa gccgccctgg catcatccct tttccgttgg
      541 ttaagataac caaacgcggg ggctcttgcc gctctctggc cagggagagg actaccggag
      601 ctgctaccgc caggtccgag aggtcacaac ccatgagtgg ctgtgtgctg caacgatcga
      661 ctctcgtgtt gaaccgccac tggcagccgg tgcacgtcgc gcctgtgcac cgtgctctgg
      721 tcatgctgtg gaatgaagcg gcatgtgtga tcgatccgga cgactttcag ctttacacct
      781 ggtcagactg ggcaaaactc gcgccccgcg agggagagcc gtttatccgg acagtccggt
      841 tccatcttcg tgtgcccgag gtcctggcac tcacccgtca cgacaaggcc cggcaccaga
      901 ccgtcacgtt cagccggcgc aacctgttca aacgtgatca ctcaacgtgc cagtactgcg
      961 gctgccagcc tggcacggag gagctcacga tcgatcacgt gatccctcgt gcccagggcg
     1021 gccaaaccac ctgggaaaac tgcgccctag cttgcgtgcc ctgcaatgca cggaaggcca
     1081 atcgcactcc tgaccaggcc gccatgaagc tgcggcgtgc tccagtgcgc cccgcgtgga
     1141 agcctctcta cgacgccagc ggtgcgcgta tcgcgagctg gtcacgcttc ttgagcgacg
     1201 cctactggaa cgtgcccctc cagaattctg gatataagat ccaacggtag aatgatcgcc
     1261 gtgcggggac ggccgtttcg gcccgaaatg gcctataacc gattgggctc cggattcgtc
     1321 aacggcgttc ggatgaatcc tgaatcagcc ggtgcccgat aatcgatgcc agccaggtcg
     1381 ggcgtggcga tcggccggtg atcgttcaac cgactttcga gcgccgcatc gaggacgcct
     1441 cccgtcaaga gcgttcgttc gaccggatag ggggacagac cggtgcggaa gaagtcctcg
     1501 atccgtagca ccaacggatt gaaaaagctg gcacccggcg gcgcgggcaa atacatcagc
     1561 gttgagacga tggtgtcttt cccgtccgag ccgctcaacc gtgcggcgat cgtcgtatcg
     1621 tcgacgtgac cgttcaagat cagcacagtg gcacgcaaac cgtcgacata ttcgacgatg
     1681 taggcgaccg ggtagcccag tttggtgggg acgtcgcgtc ccggtgcggg cgggggcttg
     1741 aagtcgcgcg tgttttgctt gatgtctcca ggattcactg agtgactacg tgagagggcg
     1801 tgctcgagta ccgaccacga ccactcaccc ttgtcgcccg cctgccagac cgcgtcacct
     1861 tccaggcagg tcacggtggc cacgccctgc ggcttgcctc tccgctccct ccgctcgacc
     1921 atgcactgga gggtctcgag cgcatggaaa ccaaagatct ccaggtcacc acgcgaggcc
     1981 acgacgacgt ctttgaactc ccggcccagg ggaatctcga gcgcggggag ccgccaggtg
     2041 accggcaagc ttgagccggc catgagcggc actttcaggc tcttagcctg gtccaccatc
     2101 tgttgcgcct cggcgcggct gtacgagagg tgtttgtcga tgaagaccgg gacggccgcg
     2161 cccgacgact tgaagacttc aagcacttcg ccaaaaaacc gcccgcgcgg atagagcttc
     2221 tggagctttt cgttgtaggg atactcgcca tgctcggcga tcaagaggac ggcatcgacg
     2281 cccagcttgc cggttcccag ggtgagagca tcggcaattg atgggctctg acgaatgcca
     2341 tgcagcctgg ccttggctcg gcccaggtcc gtcgctaccg gggtttgctc gatgaacaga
     2401 ctggcaatct cgaattccgg cttgtgcagc ccatggccgt cgtaaacggg gaatccgtcg
     2461 atgaatcgac cgacgatgtg gtacgcatgc gacaggtagt tgtagaccgt tgccagcgcg
     2521 gcgactttca gtcgcctgcc cctggccgcc gccggttgcg agtttggccc gcccaaagga
     2581 gccgaaccca cggcgcccgt caaaccagct atgcccgctg ccgccccggt catttccaga
     2641 aatcgtcgcc gcgtcgagct tcgaatcatt cgtgatactc cgcttttatg agcatgcgtg
     2701 cgaaggcggg tcgatcgcgc gatgcgacgg aaccaggata aaccaccggt cgagaggcag
     2761 caacgcctcg gctgccggtc atcgaccggc tcatgtgacg atcgccaagt gacacccgtt
     2821 gggcgcaaaa ttggcacgtt ccagaggcgt cggcgacgcc agctcgtcat gctcgctagc
     2881 tgttgacgaa tggccaccca gcccagtcgc tcgcgaagtg ttgccgttca gtacaaactg
     2941 ccgccgacga gttgtcaccg ccgcagtccc acgggacgct gtcgaagcgg ttttggcttc
     3001 tatcgtccgg cttacgagca gcctccatga ggcgatggag gttctctcgc agatcggcgc
     3061 gatccggctc ctcggcgatc gcggcagtga ggaagaggat cgcctcaaag cgatgaccca
     3121 ggcgttcagc caggcgggcc atttcttcgg aatcgcgcgc gggctgattg cggcgataga
     3181 gatcgcgata ccgcatctgg tcgcgttcaa tctccgctct tgggcgccgc ggcttggcga
     3241 gagcggtgtt cgccggcgct tgttgctcca gcttttccag acgctcgagc gcccggaaat
     3301 cttcggggac ctccgcgacc agggctgcca gttcctggcg ctcgcgatcg atatccccgc
     3361 acatggatgc gatccacgcc gagaggcagt ggactttcgc gggtgtcgcc agctcggccg
     3421 ggaggtgctt gagcgcggcc ttcgcttcat cgagccgatt cgttcgcatc gaccagtcca
     3481 gccgcgcccg ccagacggct cgatcgtcgg gacggcgcct gagacagtcg tcaagcaacc
     3541 gccgcccctc gtcgtaggag ccggccctga tcgccagatt cgcccgcccc agccagatcc
     3601 ggtcgtcgtc gggagccagg cggccgacct ggtcgaggta gtcacgaaga gtcgccgccg
     3661 gcgggatatt ccagcgcatc tccatatgga gtcgggccag gttgacggcc tgctcgaccg
     3721 ttacttcacc gtgtgcgtcc aggctctgcc atcgcaattc gatcagccgc tgggcctctt
     3781 ctgcccggcc ttcctgcacg aaattcggga tcaatatcat gcggcgcgcg gatccctccg
     3841 acccgagggg atcggtcgtg tgcatgataa agtcctcagc gtcggcgaac cgcccctgtt
     3901 caatgagcag gtcggtgcgg cccgcgatcg cgcgtccgct gtaggtagag tccggcggta
     3961 tggtggccca gtccggctat gagacgcttc ccgaacgggc tggtttccgg cacagcgggc
     4021 ggattgaaaa gttcgtgcag gtactgacct ggaggaccaa gcatcgcgac ctggttcggc
     4081 tcgagctggg taacaacgaa gagaacagca agcagtccgt tgatggcgag caacattctc
     4141 aatcgccagc gatttcgcct tgcaggtttg gctgccgggc tgaaaggtcc ggctggcaca
     4201 gccgagattc cgtgtgggga ttcgctgtct ggcgtatcag ggtcctaaaa aaaatcccca
     4261 cgatctacgt ttttggttgg cttcctttcg cacgcgacat gagccgtgtc tgaagatatc
     4321 atgaatggct atgtgagtga tcaaacctct ggagaaatcc gatgagttgc aatcaaaggc
     4381 tatgcatcca caatgccact gcgcgactgc ggaccatccg aattgaacct tgggcacggc
     4441 actatctact cggtcccgat gaaaaactgg agatcatcgc aagagctaca gaacggcgtt
     4501 caagctttcg catcgttgag gcgaacgaca cgactctgat ttacactgag cgatgtgaca
     4561 acgtccgcgt cgttcaggac ggcatttcgc aggatctctt gcgagactac gaaatcgacg
     4621 tggaaccggt ccgtccgacg cgtacgaatg ataatcccat gttcgaccga gatgtcgatg
     4681 caaactaata cttaatcact taggtcgagt gccgcgcgga cttttccccc ctttcacaag
     4741 ggggggaaag gaaaggcatc cgccactccg tattccaatt cagcgcgacg aaaatacgca
     4801 ggtctcaacc taaaagttga aacgccccct gatcttggcc tgaactacga caccgagctg
     4861 gccgatctgt tctcgatgcc tgcccgtgcc gtttcaaact cttgactcgt catgatgatc
     4921 gtgtttgcct gacccgggtc gttgggcgcg gcggggtcag caggttgttc tgagccagcg
     4981 accgccgcga tcgccggcgc cggcgactgc cagtcgagcg acatcagctc ggcccaacga
     5041 ttcagctcgg ggatggtctg caataccact ttcaggctaa ctgtgatcgg catagccagc
     5101 accaggccga tcaggcccca gagatatccc ca
//
LOCUS       DATAQL010000106         7603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1384261, whole genome shotgun sequence.
ACCESSION   DATAQL010000106 DATAQL010000000
VERSION     DATAQL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384261"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VHS97_01765"
     CDS             <1..770
                     /locus_tag="VHS97_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446946.1"
                     /translation="ATGPSGGGTNPPADGAPPANGSTSPPANAPSPGGSSPPGQGASG
                     PNQPGRPGMGQQQGGMQQQQQQQRQQQQQQQQQQEQQQRNQANNQAGGAPGRPGGQGG
                     PGGAGGTGAAGVDNSPADFHSPEGAVRAFLSALKAKDLDRLNEATALRAPVEATPKNQ
                     KLFEKIFGLSLSESELDELAKKLDGYQIAGENPPQSTARVDVILQKADTEHRGGYFRR
                     KVTVRHEKKGWGVLDIAGATEFKAPIAVPGRGMGRRR"
     gene            1163..1492
                     /locus_tag="VHS97_01770"
     CDS             1163..1492
                     /locus_tag="VHS97_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446947.1"
                     /translation="MVFSAPANLCAIFEVLVVLFQVFGIMGLCLNRLMPRSTPWSSRG
                     QIGSIIALVGLGIAGALCGRHDSEFALFAGGTMTVLLIGLTIGGGTIDKNEPARPRVT
                     TEAELAA"
     gene            1882..2706
                     /locus_tag="VHS97_01775"
     CDS             1882..2706
                     /locus_tag="VHS97_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3446948.1"
                     /translation="MMVELKRISTALGLVAALATSAMAQGSAPNANQMTADAVAGTLR
                     SSSNLAGNRIEIQAKEGVVTLTGVLGSPVLKSEALARTRYVAGVRQVVDQLQVANDGS
                     VRTVQYQPNQRVAFRQDLAGHPIGGDVVHGGGMGTGAQFGGGMGTGAQFGGGMGAGAQ
                     FSGGPTTSGPISDGGPMPEGPSGSAGATQAAQPGYPNYAWPSYAPYPNFSAVGYPTAY
                     PWQAWPNIGPFYPYPEVPLDWRAVTLRWDDGIWWLDFKKHYTRPFFTPYPFGLFAY"
     gene            3137..3757
                     /locus_tag="VHS97_01780"
     CDS             3137..3757
                     /locus_tag="VHS97_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446949.1"
                     /translation="MFRSARHRSPIASRWVRGLLAVAFLAFVALNSAGCTLGAISYVA
                     NNISPLHVTGWSIAWPIFASQSQRLEEDIKREERDRVPILDPIPTEFAPAACLDPPSE
                     EEVWNKVPKFKHGAPIFYETQRNNVRILIEKIGEKIDPAKVYPLAGPCQLVHCHYKCT
                     VYYDELYWADYPIPFNHVDHKVEVVYFDKDHLRRVGGPEQASNPPL"
     gene            complement(3896..4834)
                     /locus_tag="VHS97_01785"
     CDS             complement(3896..4834)
                     /locus_tag="VHS97_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3446950.1"
                     /translation="MTLSHWVNRARWTLPGLILFFAAMSAIPPWAAAQENATSQALRI
                     IVFGAHPDDCELEAGGTAARWAKLGYKVKFVSVTNGDIGHHQLAGAPLARRRTDEVKK
                     CAQILGIDTEVLDIHDGELLPTLENRRTITRLIREWKADAVIAPRPNDYHPDHRYTGI
                     LVQDAAFMVIVPSFCPDVPALRKNPVFLYTEDDFKKPNPFVPDVVVPIDPVIDLKVAC
                     VDALGSQFYEWNPWLFDYLSQVPTDPAARLEWTRNRVEKRYGSLANRFRPKLIELLGE
                     EKGKEVKYAEGFEICEYGSQPSAAELMRIFPFFGGK"
     gene            5072..6925
                     /locus_tag="VHS97_01790"
     CDS             5072..6925
                     /locus_tag="VHS97_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446951.1"
                     /translation="MKPLSKKRRWALSRRLGSASICGAIIVLLANREAQSQTRADLLE
                     LPDGQRVTGHLAGDSRTRIGFTAVQASAPVPLESGSVVQFNGSGPTSLASTPPFRVLI
                     GETLRLSGTLRGISQRGVRLGLSWDAPDVALPRPGVEAVVQRPGEARVLVDGFETLEK
                     SRWAIVGKPELVAAPHFVDEQSLRLPAGGTSLVHALAEPIAAGRFDLAFLDDGAVVAG
                     QLWSIELLFQGSSGVSATRVLLGWAEESLAVESPNGLAVQRLARTPGWHRFSLRFSPT
                     QTEVSVDGKELAHGKGPDGPLVTIRLASSAPAQAVPPPGLAGHFDDLQLIRFAEPPDS
                     FELDVAQDEARLVMGDQLYGEIKHSDGERVNMTVDGEPISLAWSDVSGLYFRCIPARG
                     NVIEGLLARLEWRTSPGEEPDNVDFAEGAIQSVSDKAVTLDTPYAGVLSIPRERVRRL
                     VVRGHGRRLLIDQAAHHLGDEYSATAPLLNPRQPEGDRLERAIELTEIPDQPCFLVLD
                     VFQLVGEDNDPRWSQRIREGELRTYVAINGKRIDYLNRYVKKSNDVPEQIAVPIPKGA
                     LKRGKNTIRLELTGMATKEKELDDFGLLQMALEFRSSPSRGQEPKAETGPP"
     gene            6938..>7603
                     /locus_tag="VHS97_01795"
     CDS             6938..>7603
                     /locus_tag="VHS97_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446952.1"
                     /translation="MKTLTRSMVRHAHLIAREVGAKVILLHADVVEDDCDLSALIEDV
                     GFRVVLVSRRPGFRAPDEWKDLCKVIQLPDIAMTRTGQIKVAILLVAAERLIQAGDRI
                     LCLTGVDLSEALDTIMVLDMGTEIELFSASTADPLPADVTPTVFERALTLAGELGVEG
                     REGRPVGTIFVIGDSDRVLAQSSQLVINPFHGYAENERNLLDPRLEETIKEFSAIDGA
                     FVVR"
BASE COUNT         1519 a         2316 c         2295 g         1473 t
ORIGIN      
        1 tggcgaccgg cccatccggc ggcggcacca atccgcccgc cgacggcgcc ccgccagcca
       61 acggatcgac gagtcccccc gcgaacgcgc cgtccccagg cggctcatcc cccccggggc
      121 aaggtgccag cggtccaaat cagccgggga gaccgggcat gggtcagcag cagggaggaa
      181 tgcaacagca acaacaacaa cagcggcaac aacagcagca gcaacaacaa cagcaagaac
      241 agcagcaacg aaaccaagcg aataatcaag ctggcggagc gcctggccgg cctggcggcc
      301 aaggaggccc cggtggtgcc ggcggaaccg gtgccgcggg agtcgataat agcccggccg
      361 attttcattc tccggaaggt gccgtcaggg cgttcttgag cgccctcaaa gccaaagacc
      421 tggaccgact taacgaggcg accgcgcttc gcgccccggt cgaggcaacc cccaaaaacc
      481 aaaaattgtt cgagaagatc ttcggtctaa gcctttccga gtccgagctg gacgaactcg
      541 ccaagaagct cgacggctac cagatcgccg gcgagaaccc gccacaaagt acggcacgcg
      601 tcgacgtcat cctccaaaaa gcggacacag aacatagggg cggctacttc cgccggaagg
      661 ttaccgtgcg gcacgaaaag aaaggctggg gcgttctcga tatcgccggt gcgacggaat
      721 tcaaagcgcc cattgccgta ccaggtagag ggatgggcag acgccgctga ggcgcgattg
      781 agtttatcgc gggcgggcct gcttggcctt ccgctcattc gttcaagctc tcgtgccaac
      841 caacgcgagc ccgagtccgc gtaatcggtc caaatgatgc acgcgaccat gaatgacaaa
      901 atgtaagatt gacgaacccg cttgtgtaac ttttccaccc gcgcctgcaa aattctttgg
      961 gcgcgggtgt tttttttaaa gttttgcggt tgaatggccg gggttcgggc ccattaagcg
     1021 aatctggggc ttttggtccg gcatgtgctc aaggaccaag accaagcgac cgctgcaagc
     1081 tgggtagagc agcaactctc tgtcgcaatc ggacttcaca cacttcgaca tctcccacgc
     1141 aggaaccaag gaaggagcct gtatggtttt cagtgcacca gcgaatctgt gtgccatctt
     1201 tgaagtcttg gtcgttttgt ttcaggtatt tggcatcatg ggtctttgcc tgaatcgact
     1261 aatgccccga tccacgcctt ggtcgtcgcg tgggcaaatc gggtccataa ttgcgctcgt
     1321 cggtcttggc atcgcgggcg cgctttgcgg ccgccacgat tcggaattcg cactcttcgc
     1381 cggtggaaca atgacggttc tgctgatcgg tttgacaatc gggggtggaa cgatcgacaa
     1441 gaacgaaccg gcacgtccgc gtgtgacgac cgaagccgag ctcgccgctt gaacgagaaa
     1501 aatcggcgta agctttttct gaaccaaaga cttcgacgca tcggggccga tcggccccga
     1561 ttttttttgt ccgtcatttg acaggcccca aaccgcgcgc aatagttgcg gcgacgtttg
     1621 gaatcgacgg gccgtctccg cctgcctgca catctttgtc tcaacacctc gacagagccc
     1681 gttcctgctc gcgtcgagtc tcgtgggtac gattcgaaca gcctcgagcg gtttcggccg
     1741 cgacgggcgc ccgttcggac tgcgccccct gatccatatg aaacgtatgt cacgatggac
     1801 cattctccga cccgaacgac gcgtcccgca cgccgttcgc cttctggtcg agtcggatcc
     1861 aagcgggaaa aggacaagcc catgatggtt gaactgaaac ggatttcaac cgcgcttggt
     1921 ctggtggccg ccctggccac gtcggcgatg gctcaagggt ccgcccccaa cgcgaatcag
     1981 atgaccgcgg acgctgttgc cggcacgctg cgctccagca gcaacctcgc cggtaatcgc
     2041 atcgagatcc aggcaaagga gggcgtggtt acgctgaccg gcgtgctcgg tagcccggtc
     2101 ctgaaatcgg aagcgctggc gcgaacgcgc tatgtcgcgg gcgttcgtca ggtcgtggat
     2161 caattgcagg tcgcaaacga tggatcggtt cgcacggtcc agtatcagcc caatcagcgg
     2221 gtggctttcc gccaggatct cgccggccat ccgatcggcg gcgacgtcgt acacggcggc
     2281 ggaatgggaa ccggagccca gttcggcggc ggaatgggaa ccggagccca gttcggcggc
     2341 ggaatgggag ccggagccca gtttagcggc ggcccgacga cgtccggccc gatcagcgat
     2401 ggcgggccga tgccagaggg gccgtctgga agcgctggcg cgacgcaagc cgctcagcca
     2461 gggtatccca actacgcctg gcccagctat gctccttatc ccaacttctc ggcggtcggc
     2521 tacccgactg cttacccctg gcaggcctgg ccgaacatcg gcccgttcta cccctatccc
     2581 gaagtcccgc tcgactggcg cgccgtcact cttcgttggg acgacggcat ctggtggctg
     2641 gatttcaaga agcactacac ccggccgttc ttcaccccct atccgtttgg gctgttcgct
     2701 tactgagcgg tatccggttg attcccgcga tcggcaagcc caacgagagc cggctggaag
     2761 gcattttgct tccagtcggc ttattttgtc gtatccggca gttcgaaaag aacgcccaca
     2821 ctctctatgt caacgtaatt tgcgaaagaa cgaccaccct attcgccgat gtccatctcc
     2881 agtgaaccaa gtcgtacgcc tgagtcgttt ggagtgctat agagctccgc gagaaattgt
     2941 caacgcaaac gagaaatttc tcactcgctc agtcgttctc gttcgataac tagacccatg
     3001 gaccggtcgt cggattgtgg caagtcgccg gtttgatcgg tcaatcgctc gatgttagcg
     3061 gtcccggcgc cccaaggccg gaggggcaag aggcctctcg gcgggttcgc gcacgtccca
     3121 aggaggggag aagacaatgt tccgatccgc gcggcaccgc agcccgattg cctcgcgctg
     3181 ggttcgcggc cttttggcgg ttgccttcct ggcttttgtc gctctcaata gcgccggatg
     3241 tactctcggc gccatctcgt acgtagcgaa caacatctcg cccttacatg tgacaggctg
     3301 gtcgatcgcg tggccgatct tcgcgtcaca gtcacaacga ctggaagagg acatcaagcg
     3361 cgaagagcgc gacagagttc cgatcctcga cccgattccg acggagtttg ccccggcggc
     3421 ctgcctcgac ccaccgagtg aggaagaggt atggaacaag gtgcccaagt tcaagcacgg
     3481 cgccccaatt ttctatgaaa cccagcgcaa caacgtcagg atcctgatcg aaaaaatcgg
     3541 tgagaagatc gacccggcca aggtctatcc gctcgccgga ccctgccagc ttgtccactg
     3601 ccactacaag tgcacggttt attacgacga gctgtactgg gcggattatc cgattccgtt
     3661 caaccacgtc gatcataagg tcgaagtcgt ttacttcgac aaggatcacc tgcgtcgagt
     3721 cggcggcccc gaacaggcct cgaatccgcc gctttgagtg ctctacagaa cgaactgtaa
     3781 aaccatcgcg ggggaccggc tcccagggca atgatgctcg ggatacctgt cccctgcggc
     3841 gttttttggg tcggggcgag tttgccatcg ctgggccaat agcgcaaacc gagattcact
     3901 tgccgccaaa gaaggggaag atccgcatca gctcggccgc ggaaggctgg gagccgtact
     3961 cgcagatctc aaagccctcg gcatacttga cttccttgcc tttttcttca cccaagagct
     4021 cgatcagctt ggggcggaac cggttggcca acgagccata gcgcttttcc acgcgattgc
     4081 gcgtccactc caggcgggcc gctggatcgg tgggaacttg ggagagatag tcgaaaagcc
     4141 atgggttcca ctcgtagaac tgagatccca gggcgtcgac gcaggcaacc ttgagatcaa
     4201 tgacagggtc gatggggacg accacgtcgg gtacgaatgg gttgggtttc ttgaaatcgt
     4261 cctcagtgta gagaaacacc gggttttttc tcaatgccgg cacatcgggg caaaagctcg
     4321 ggacgatcac catgaaggcc gcgtcctgga ccaagatccc agtgtagcga tggtccgggt
     4381 gatagtcgtt ggggcggggt gcaatcaccg cgtcggcctt ccactcacgg atgagccgcg
     4441 tgatggttcg ccgattttcg agggtcggca agagctcgcc gtcgtggata tcgaggacct
     4501 cggtatcgat tcccagtatt tgcgcacact ttttcacctc gtcagtgcgg cgacgtgcta
     4561 ggggggctcc ggcgagttgg tggtgcccga tgtcgccgtt ggtgacgctg acaaacttca
     4621 ccttgtatcc caatttggcc caccgggcgg ccgtgccgcc ggcttccagc tcgcagtcgt
     4681 ccggatgggc tccgaaaacg atgatccgca gagcttgact tgtcgcgttc tcctgggccg
     4741 cggcccaggg tggaatcgcg ctcatcgccg caaaaaacaa aattagaccg ggcaatgtcc
     4801 accgcgcccg attcacccaa tggctcaaag tcattttgac cgctcccaat cgttaagatt
     4861 tcgatcgcgg gaggtctccc gcgaccaggc aacgacgctg gcttgtcctc ccagcctatc
     4921 aacctgctca cgcgagtcac tgggggagtc gaattcgtta ccgaaacgat cgccgctaca
     4981 catcgcgatc gcgcgagtct cttgttgcta ttttctcaac tgcaagcgct cgacttgccg
     5041 gttcaacttg cgacctcgta aaatcaagtt catgaagcca ctctcgaaaa aacggcgctg
     5101 ggcgctctcg cggcggctgg gttccgcctc aatttgtggc gcgatcattg tcttgttggc
     5161 aaaccgggag gctcaaagcc agacgcgggc cgatctgctg gagcttccag atgggcagcg
     5221 ggtgactggg catctggccg gcgacagtcg gacgaggatc ggctttacgg cagtacaggc
     5281 gtctgcgcct gtgccacttg aaagcggttc agtcgtccag ttcaatgggt ccgggcccac
     5341 gtccctggcg agcacgccgc cgttccgcgt cttgatcggc gagactctgc gactctcggg
     5401 taccctgcgc gggatcagcc agagaggcgt acgactcggc ttgagctggg atgcccccga
     5461 cgtcgcgctg ccgcggcccg gtgttgaggc cgtcgtccag cgtccaggcg aggccagggt
     5521 gcttgtcgat ggattcgaga cgctcgaaaa gtcgcggtgg gcgatcgtgg gaaaaccgga
     5581 gcttgtcgcc gcaccccatt tcgtcgacga gcaaagtttg cggttgcccg ccggcggaac
     5641 atcgctcgtc cacgctctcg ccgagccgat cgcggccgga cgttttgacc tggcattcct
     5701 ggacgacggg gcggtcgtgg ctggccagct ctggtcgatc gagctgttgt ttcaaggatc
     5761 ctccggtgtg tcggcaacgc gcgtgttgct agggtgggcc gaggagagcc tggccgttga
     5821 gtcgcccaac ggtctggccg tgcagcgact ggcccgcaca cccggctggc accggttctc
     5881 gctcagattc agccccactc aaaccgaagt ttccgtcgac ggcaaagagc tcgctcacgg
     5941 caagggcccg gatggcccgc tcgttaccat tcgtctggcc agctcggctc ctgcgcaggc
     6001 ggtgcccccg cccggcctcg cagggcactt cgacgatctg caattgatcc gttttgccga
     6061 gccgccggac agcttcgagc tcgacgtcgc ccaggacgaa gccagactgg tcatgggtga
     6121 tcaactctat ggggagatca aacacagcga cggtgagcgg gtgaacatga ccgtcgacgg
     6181 cgagcccatc tcgctcgcat ggagcgacgt ttccggtctg tatttccgtt gcattcccgc
     6241 cagagggaac gtgatcgaag ggctgctggc acgcctcgag tggcggacgt ctccggggga
     6301 agagccggat aacgtcgatt tcgccgaagg agcgatccag tccgtttcgg acaaggccgt
     6361 caccctggat actccgtacg ccggcgtgct gtcgattccc agagaacggg ttcggagact
     6421 ggtcgtacgc gggcacggac gccggctctt gatcgaccag gcggctcatc accttggcga
     6481 cgagtactcc gcgactgcgc cattactcaa ccctcgacag cccgaaggcg accggctcga
     6541 gcgcgcgatc gagctgaccg agatcccgga tcagccgtgt tttcttgtcc tcgacgtctt
     6601 tcagctggtc ggcgaagaca acgacccccg gtggtcgcag cgcatccgtg agggagagct
     6661 ccgaacctac gtcgcgatca acggcaagcg cattgattac ttgaatcgtt atgtcaagaa
     6721 gagcaacgac gttcccgagc agatcgcggt ccccatccct aagggggcac ttaagcgagg
     6781 aaaaaacacg atacggttgg aactgactgg catggccacc aaggaaaagg aactggacga
     6841 tttcgggctt ctccagatgg cgctcgagtt tcggtcgtcg ccgagccgtg gccaagagcc
     6901 taaagcagag accggtccac catgaaggcg atgccacgtg aagacgctga cgcgaagtat
     6961 ggtccgtcac gcgcatttaa tcgcccgaga agtcggcgcc aaggtcatct tgctccatgc
     7021 cgacgttgtc gaggatgact gcgacctgtc ggctttgatc gaggacgtgg gattccgcgt
     7081 ggtcctggtc tcacgacgac ccggcttccg cgcccctgat gaatggaaag acctgtgcaa
     7141 ggtcattcag cttcccgaca tcgcaatgac gcgcaccggc caaattaagg tcgccattct
     7201 gctcgttgcc gccgaacgtt tgatccaggc cggtgaccgc atcctttgtc tcacaggtgt
     7261 cgacctgtct gaggcgctcg acacaattat ggtcctcgac atggggaccg agatcgagct
     7321 gttctcagcc tccacggcag acccgctgcc agccgatgtc acaccgaccg tgttcgagcg
     7381 cgccttgaca ctggccggtg aactgggcgt ggaaggacga gaaggacgac ccgtgggaac
     7441 gatattcgtg attggcgaca gtgaccgcgt actggcacaa agcagccagc tcgtgatcaa
     7501 cccgtttcac ggctatgccg agaatgagcg caatctcctc gaccctcggc tggaagaaac
     7561 aatcaaggaa ttctcggcaa tcgatggcgc gttcgtcgtg cgt
//
LOCUS       DATAQL010000107         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3063861, whole genome shotgun sequence.
ACCESSION   DATAQL010000107 DATAQL010000000
VERSION     DATAQL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3063861"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VHS97_01800"
     CDS             <1..909
                     /locus_tag="VHS97_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX3446953.1"
                     /translation="FSHAHPVLKRIKPGETVATKTVDSAGLDEKDEKRSEPSNPLTGP
                     FFVEGAETGDALVVRLKKVRMNRDWGWSAYRLGLFSLTPETVEHIYPNAVHPDLVIKG
                     RATIVPWDLDRKANTVRLRTPKSQVHPLEFPAKPMLGCIGVAPAGDFAPTSGPSGTYG
                     GNLDYNKIGEGATVYLPVYHTGGLLFVGDGHALQADGEPTGTGIETSMDVEFVVDLKK
                     GAKLTGPRVETADEIISIGSQPEFASALDNGLKIATSDMAGWLAKDFGMEPWAAHLLI
                     GYQGRYDVVTVAGSMALCLPKNRLPK"
     gene            1361..>2303
                     /locus_tag="VHS97_01805"
     CDS             1361..>2303
                     /locus_tag="VHS97_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3446954.1"
                     /translation="MSRRSSSQPLSYRSPRNVQPECAPRGGRVRFPFLSWKRIVGLGV
                     LAWVPLYLADGTTPAPGKADHSVLGKHSGRIISVAFNPSGRWLAASGGTRSVSLWDMS
                     CREPAMALEPAPEPEVAFMNCLAFTPDGLNLAVANDRGSVSMWDVASGNLWHNLRAST
                     QSVRSLAFSPDGRLLATGISDGSIVLWDATMLCERAVLLGSRRQINCVAFSPDGRTLA
                     SASTDGRTKLWDISSGENIRTLKPNANDPPAIQCVAYSPDGRILATACAQSGVALWDE
                     STGRLLVTCGECGPGAMTLAFSPVGGMLAWGATDGRIE"
BASE COUNT          433 a          688 c          744 g          438 t
ORIGIN      
        1 ttctcgcatg cgcacccggt cttgaagcga atcaagcccg gcgaaacggt ggcgacgaag
       61 acggtcgatt cggcgggtct cgacgagaag gacgagaagc ggtccgagcc gtccaacccg
      121 ctaactgggc cgttcttcgt cgaaggggcc gagacggggg acgcgctcgt ggtgcgattg
      181 aagaaggtgc ggatgaatcg cgactgggga tggtcggctt accggctggg ccttttttcg
      241 ctgacgcccg agacggtcga gcacatttat ccgaacgccg tccaccccga ccttgtcatc
      301 aagggacgtg cgaccatcgt cccatgggac ctcgaccgta aagcgaacac cgtacggctc
      361 cggacgccga agagccaggt tcatccgctg gagttcccgg ccaaaccgat gctcggttgc
      421 atcggcgtgg cgcccgcagg cgattttgcg ccgacttcgg ggccgtcggg tacctacggc
      481 gggaaccttg attacaacaa gatcggcgaa ggggcgacgg tgtatctgcc ggtctatcac
      541 accggcggcc tgctgttcgt cggcgacggc cacgccctcc aggccgacgg cgagccgacg
      601 gggaccggga tcgaaacttc catggatgtg gaattcgtcg tcgatctgaa gaaaggcgcg
      661 aagctgaccg ggccgcgagt cgagacggcc gatgagatca tcagcatcgg ctcgcagccc
      721 gagttcgcca gcgccctcga taatggcctc aagatcgcga cgagcgatat ggccggctgg
      781 ctcgccaaag atttcgggat ggaaccgtgg gccgcacact tgttgatcgg ctaccagggg
      841 cgttacgacg tggttactgt cgccgggtcg atggccctct gcctgccgaa gaaccggctg
      901 ccgaagtagg cggacccacc tacagcctga tcattgacca gaatgtcgtc ggcgatcgga
      961 cgaccaggtt actgctcgtg gaaacgatcc aaaaatcgcc gtagcgagca tgctgcgctt
     1021 tggcttgcgt cacatgctcg catccgatcg aggtgtcgac tccaatacaa cccgaatgct
     1081 ataggtcaga caaacccatc accgaccgcc gaatgaatcg aaatattcga cgcgtccgga
     1141 cgaattcctg atgtgacctt ctacgcattg tagtaacttg cctctacgtt gtgtagtaag
     1201 gtgttgctag acacacctgc ggaccaagca ttttgccgcc tggccggggg caatcagcgc
     1261 gggccagcgc gagaggatgt gccggttctt cgccaggtaa ttcgacctgt acatcctgtg
     1321 ggccgagttt gagggcctgc tggaggtgga ggagtccgcc atgtcgcgcc gatcgtcatc
     1381 tcaaccactg agctaccgca gtccccggaa tgtccagccc gaatgcgcgc cgcgcggcgg
     1441 ccgggttcga tttccgtttc tgagctggaa acggatagtt gggctcggag tccttgcctg
     1501 ggtgccgctc tacctggcgg acgggacaac tccagccccg ggtaaagcgg atcacagcgt
     1561 gctcggtaag cactcgggca ggatcatttc tgtcgccttt aacccgagcg gtcgttggct
     1621 agctgcgtcg ggaggcacga ggtcagtgtc actctgggat atgagctgcc gcgagccggc
     1681 catggccttg gagcccgccc ccgagcccga agtagccttc atgaactgcc tcgctttcac
     1741 acctgatggg ttgaatctgg ctgttgcgaa cgatcgtggg tcggtgagca tgtgggatgt
     1801 ggcctccggg aacctttggc ataaccttcg ggccagcacc cagtcggtcc gctctctggc
     1861 attttcgccc gatggccgat tgctggcgac ggggatcagc gatggtagta tcgtgctatg
     1921 ggacgcaacg atgctgtgtg agcgggccgt cttgctcgga agtcgccggc agatcaactg
     1981 tgtcgcgttc tcacccgacg gccgcaccct cgcctccgcc agcaccgatg ggaggacaaa
     2041 actatgggac atctcatcag gagagaacat ccggaccctg aaaccgaacg cgaacgatcc
     2101 cccggcaatc cagtgcgtgg catactcgcc cgacgggcga attcttgcga cggcctgcgc
     2161 tcagtcgggg gtcgcgcttt gggacgagtc aacggggcgt ctgctggtta cctgcgggga
     2221 atgcgggccc ggtgcgatga ccttggcatt ctcgcccgtt ggcgggatgc tcgcctgggg
     2281 cgcgaccgac ggcagaatcg agc
//
LOCUS       DATAQL010000108         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_163113, whole genome shotgun sequence.
ACCESSION   DATAQL010000108 DATAQL010000000
VERSION     DATAQL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163113"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VHS97_01810"
     CDS             <1..209
                     /locus_tag="VHS97_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446955.1"
                     /translation="ATRRPGARTIQAKAVVVGRAAAESPGAGCGAGRDRMTSVLSRGS
                     SISRWRRWIRDRGRNESATDESAA"
     gene            206..508
                     /locus_tag="VHS97_01815"
     CDS             206..508
                     /locus_tag="VHS97_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446956.1"
                     /translation="MSRQTISPTVGQTSQPFTTAAFTVFVRAGAGTSPDRRRHALGES
                     DGSLHIAGSLEKTRCPWFLSPSNQALARAPFPSGMIGRSPEAPIPRSTRKAGLTDG"
     gene            complement(898..1299)
                     /locus_tag="VHS97_01820"
     CDS             complement(898..1299)
                     /locus_tag="VHS97_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446957.1"
                     /translation="MKAILSMALVLGVCGLAGAADDKVVDPVGTWKCEYEIGGQQRTS
                     TLKIKKDGDNLAGTMSWPDQDGTKLKDLKLKDGTLTFSAERKLAGMDDGITVEYKLKI
                     DEDKLKGKGAAEFGGEKREWDIEGKREKNDK"
     gene            1610..2374
                     /locus_tag="VHS97_01825"
     CDS             1610..2374
                     /locus_tag="VHS97_01825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3446958.1"
                     /translation="MKSTVEQIRARFDNDVERFSNLETGQSATIDAPLVLELISQAAA
                     ATNPGATDLLDVGCGAGNYSLKLLQSLPDLNLTLIDLSRPMLERAQQRLRPATKGHIE
                     AFQGDVRDLDLGEARFDVIVAAAVLHHVRTDAEWEAVFAKFFKALRPSGSIWISDLIE
                     HSSAAIQEMMWRRYGEYLVGLKDEAYRDTVFAYVTREDTPRPLMYQLDLLRKVGFRQI
                     EVLHKTSVFAAFGAWTYADRIAAVPAGEVDDSERSA"
     gene            complement(2772..2996)
                     /locus_tag="VHS97_01830"
     CDS             complement(2772..2996)
                     /locus_tag="VHS97_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446959.1"
                     /translation="MSRDHMKDVEFSEMCLKLPRTPIEDDISYRAALEILDRLFARDD
                     LRTPAELEYFRTLAQMASEYEMKRISNSSS"
     gene            complement(3018..4607)
                     /locus_tag="VHS97_01835"
     CDS             complement(3018..4607)
                     /locus_tag="VHS97_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3446960.1"
                     /translation="MVLFFLWVLTLTAPLIALMLSAGCAALPKPVSETGFVTTAPNAT
                     DAAVPPDVARQYWKRDRSREIKSNGYSREGFFMPATDLGSRSTAQERLILLVLATVQF
                     TAIVDFMVIMPLAPQLKRAIGLTTDRFALVVAAYTVAAGLAGLISTLFLDRFARRPTY
                     LVLFAGFLLGTLACGVAPNFVTLMAARGLTGAFGGILGGMAMTIIGDVFPEHRRGAAT
                     GILMMSFSMASVLGVPIGLTLGQEFGWHIPFLVLAALCVPFFILAACVLPRLDGHLKG
                     QGRAEPLAQLKAIFLDANHLRAFALTSALMISGFSVLPFLSAYLVANVGVDEKHLRWA
                     YVAGGIVTLVGMPTIGRMADRFGKLRVYRFVVPANAALLLMVTSLPPLPLALVLLAMS
                     ATMLSNGGRMVPAMAMIASSVVPRLRGGFMGANSAIQHLSSSLGVMVAGFILFEPKNG
                     PIERFPVVGLMAAVVSLSSLWLAGRLRVEASAADDEAVEAAKAGIATAHGQEKVQMRV
                     VPTTVVFSRDHQVAPISARTG"
     gene            4855..5385
                     /locus_tag="VHS97_01840"
     CDS             4855..5385
                     /locus_tag="VHS97_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3446961.1"
                     /translation="MSRSPSDQSSDSQSLVDAFGRLMPVFKRWLELKYAKGTMSYARL
                     KLIGMLHVKGPMIMSELGARLDVSARNVTKLVDALEHEGLVRRVAHCTDRRATVIETT
                     PEGSKVGEQVWGEHRRSMSTLFDELSDVDRADLLRTIVRLHAVLERRMAAAGLNPSRS
                     AEVPRASQAPGEANPG"
     gene            5585..>5767
                     /locus_tag="VHS97_01845"
     CDS             5585..>5767
                     /locus_tag="VHS97_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446962.1"
                     /translation="MRRIRTGLVLFSAAGMLGAAALAETKVEIGNVHLCCPGCVKAVD
                     SALAGVEGVTGVCDQKA"
BASE COUNT         1136 a         1868 c         1748 g         1015 t
ORIGIN      
        1 acgctaccag gcgtcccggg gcacgtacga tccaggcgaa ggcggtcgtc gtgggcaggg
       61 ccgccgccga gtcgcccggt gcaggctgcg gtgccggccg cgacaggatg accagcgtgc
      121 tcagtcgagg ctcgtcgata agccgctggc ggcgctggat cagggaccgg ggccgaaatg
      181 agtctgcaac agacgagtcg gccgcttgag caggcaaacg atcagcccaa cggtgggcca
      241 gacatcccag ccattcacga cggctgcttt caccgtattc gttcgcgcgg gtgccggcac
      301 atcgccggat cgacgacgcc acgcgctggg cgagtcggac ggctcacttc acatcgctgg
      361 ctccttggag aagacgcgct gcccgtggtt cctcagtccg tcgaaccagg ccctggccag
      421 agcgccgttc ccctcgggga tgatcggccg ttcacccgaa gccccaattc cgcgatcgac
      481 gcgaaaggcc gggcttaccg atggctaacg accaaatcga acgacctcta ggccgcactg
      541 gttgatgagc cgatggcaga gctccacgag tgcgcgatgc cgatcgggaa gatcttcgct
      601 tcagcataac gcctgcgcgc ccgggcacaa ggcgttgtca gagtcaatga atggagacaa
      661 acggtcactt ctgaccccca gttcagttat gcgcgctctg cgtacgaaga cgcacggatt
      721 tcgagataac gccgatcaat ccgacaatcg ccgtccaaga ctacctctcg gaaatcctgc
      781 ttcggtcgat ccgaccgacg cgcgcaacgg ggaccctgca tttcgggggc gaagaaacga
      841 cgataccttg gcccgcgtcg tccatccgcc gtcggaaccc tcaagactgc gcgactacta
      901 cttatcgttc ttctccctct tgccctcgat atcccattcc cgcttctcgc ctccgaattc
      961 tgccgcgccc ttccccttga gtttatcctc gtcaatcttc agcttgtact cgacagtgat
     1021 gccgtcatcc atcccggcca acttccgctc ggcggagaat gtcagcgtac cgtccttcag
     1081 cttcaggtcc ttgagcttcg tgccgtcctg gtccggccag ctcatcgtcc cggcgaggtt
     1141 gtccccgtcc ttcttgatct tcaaggtgga tgtccgctgc tgtccgccga tctcatactc
     1201 gcacttccat gtcccgaccg gatctaccac cttgtcgtcc gccgcgcccg ccaggccgca
     1261 taccccgagg accaacgcca ttgagagaat cgccttcatg atcgcctccg agatcgtggc
     1321 gcaacggacg cagtccaacg tagaccgcca cccgccgctg gcggcgatta tacccgtgcc
     1381 gcgaccggtg gtcaggacta tgagcgccga aaccctgtcc gtcggcaacc ggatgcacgg
     1441 gtcacgcgtg agccatatcc cactcgttcg gcggcgtctc gcccgtcttg accgggcggg
     1501 tgatcaccgc gctgcaccag atcatccggc gaccgaacct cggcgggctt ggccgtgtct
     1561 catccttgca caacctcgtt ctcacacact cgggacgcgc gagactcgca tgaaatcgac
     1621 cgtcgagcag atccgggccc gattcgacaa cgacgtggag cggttcagca acctggagac
     1681 cgggcagtca gccacgatcg acgcgccgct agtcctcgag ttgatttccc aggccgcggc
     1741 cgccacgaac ccgggtgcga cggacttgct cgacgtaggt tgcggcgcag gcaattacag
     1801 cctcaaactt ctccagagcc tccccgacct caacctcaca cttatcgacc tgagccggcc
     1861 gatgctcgaa cgcgcgcagc agcgcctccg gcccgcgacg aaaggacaca tagaggcctt
     1921 ccagggcgac gtccgtgacc tggacctcgg cgaagcccgc ttcgacgtca tcgtggccgc
     1981 ggccgtgctg caccacgtcc gaacagacgc ggaatgggag gcggtcttcg cgaagttctt
     2041 caaggcgctc cggcccagcg gctcgatctg gatttccgac ctgatcgagc actcctccgc
     2101 cgcgatccaa gagatgatgt ggcggcgcta cggcgagtac ctcgtcgggc tgaaggacga
     2161 ggcctatcgc gacaccgtct tcgcctacgt cacccgggaa gacacgcccc ggccgctgat
     2221 gtaccagctc gacctgctgc gcaaggtagg attccgccag atcgaggtcc tgcacaagac
     2281 cagtgtgttc gcggcctttg gcgcgtggac atacgcggat cggatcgccg cagtccctgc
     2341 aggagaagtg gacgactcgg aaaggtcggc ctaaagaagc ctggatcgtc gcgtcgagca
     2401 gggccacggg catggtcgcg gctgccgccc ccggggcgag cagggtcgcc ggcagcgcca
     2461 ggccgcggca ggttaaccgg acccggagcc gctcccgcgg cagggagagc cggggtaaag
     2521 ggtcagggcc gtcgctcacc tccggaacag cagcagtcgc aaccgagaca ggttctctca
     2581 agctacatga cgtgcaagtt ctcgctcagt ctactggtct aactcttgcg cgcgtcggtc
     2641 cgacgacacc gaagcgcgct actgccaggg cgagcaagcg accgccaggg agcttcccca
     2701 agctggctca tgtcagccag caatactgga tgtgatcatc ggtatcaaaa gggtcatcca
     2761 agctattgag gtcatgacga tgagttactg atgcgtttca tttcatattc agaagccatt
     2821 tgtgccagcg tccggaaata ctccaactcg gccggagtcc gcaggtcatc gcgtgcgaag
     2881 agacggtcca ggatctcgag ggcagcccgg tacgaaatgt cgtcctcgat cggcgtccgt
     2941 ggtagcttca ggcacatttc cgagaattcc acgtctttca tgtgatcgcg gctcattgcc
     3001 caatcctcct actcaggcta tcccgtgcgg gccgagatcg gggcgacctg gtggtctcga
     3061 gaaaagacga ccgttgtcgg gacaactcgc atttgcactt tctcttgtcc gtgtgctgtc
     3121 gcaatccccg ccttggccgc ttccacagcc tcatcgtccg cggcactagc ttccacccga
     3181 agtcggccgg caagccacaa gctcgagagc gagaccacgg cagccatcag gccgacgacg
     3241 ggaaagcgct cgatcggccc attcttcggt tcgaacagga tgaagccggc caccatgacc
     3301 ccgaggctcg acgagagatg ctgaatggcc gagttagccc ccatgaagcc gccccgcagg
     3361 cgcgggacga cactggaagc gatcatcgcc atcgccggga ccatacgccc gccgttgctg
     3421 agcatcgtgg ccgacatggc gagcaacacc agggcaaggg gcaggggggg taagctagtc
     3481 accatcaaga ggagtgcagc gttggctgga accacaaacc ggtacacgcg gagcttgccg
     3541 aaccggtcgg ccatccggcc aatggtcggc attccgacca gtgtgacgat cccgccggca
     3601 acgtaagccc atcgcaagtg cttctcatcc acgccgacat tggcgaccag gtacgcgctc
     3661 aagaacggga ggacgctgaa gccgctgatc atcagggccg atgtgagagc gaaagcccgc
     3721 aggtggttcg catccaggaa gattgccttg agctgggcca gcggctccgc acgaccttgt
     3781 ccctttaaat gcccatcaag ccggggcaac acgcaggctg ccaggataaa gaaggggaca
     3841 caaagagccg ccaggacgag gaatggaatg tgccagccaa attcctgccc gagcgtcagg
     3901 ccgattggca cgccaaggac cgaggccatc gagaacgaca tcatcaggat tccggtggct
     3961 gctccacggc gatgctcggg gaagacatcg ccgatgatcg tcatcgccat cccgcccaaa
     4021 attcccccga aagcgccggt caatccgcgg gccgccatga gcgtgacgaa gttcggagcg
     4081 acgccgcagg ccagcgtccc cagcaggaac ccggcgaaca gcaccaggta cgtcggcctc
     4141 cgcgcgaacc ggtccaggaa cagcgtcgat atcagccctg ccagccccgc cgcgacggtg
     4201 taagccgcca cgaccagggc aaagcggtcc gtcgtcaggc caatcgcccg tttgagctga
     4261 ggtgccagcg gcatgattac cataaagtca acgatcgcgg tgaactgcac tgtggccagg
     4321 acgagcaaga tcaggcgctc ttgtgccgtc gatcgactgc cgagatccgt agcgggcatg
     4381 aaaaatcctt ccctagaata gccatttgat ttgatctcac gtgatcggtc ccgcttccag
     4441 tactgccggg cgacgtcagg tggaaccgcg gcatccgtcg cattcggcgc ggtcgtgacg
     4501 aagccggttt cggagaccgg tttgggcaag gccgcacaac cggctgatag catcagggcg
     4561 atcaagggcg ccgtcagggt caggacccac aagaaaaaga gcaccatttc ccgcgcctcc
     4621 attgttctgg gcgtgactgg ggtgctggtc cgttgagcgg ctcgatcagg acgcggcaac
     4681 tccctttggg caccactggg ctagcccatg tcggcctctt cctggagccg acttatcgcc
     4741 ggggcgggcg atgggtttgc tgtcctgggg gcctcctgcc cgtttataat gtctgtcgaa
     4801 ctacttccct agcggataat gtactagttc agcattatta ggatcgtctc cgaaatgtca
     4861 aggtctccat cggaccagtc gtccgattcc cagtccctgg ttgatgcctt cggtcgcctt
     4921 atgccagttt tcaaacgctg gctcgagttg aagtacgcga agggaacgat gagctacgcc
     4981 cggctcaagc ttatcggcat gctgcatgtg aaagggccga tgatcatgag tgaactgggt
     5041 gccaggctcg acgtctcggc ccgcaacgtt accaagctgg tcgatgcgct cgagcacgag
     5101 gggttggtcc ggcgagtcgc ccattgcacc gaccggcgcg ccaccgtgat cgaaaccact
     5161 ccggaaggga gcaaggtcgg cgagcaggtt tggggagaac atcgcaggtc gatgtcgacg
     5221 ttgttcgatg agttgagcga cgttgaccgg gccgatctgc ttcgaacgat cgtccgactc
     5281 cacgccgtgc tcgagcgacg catggccgcg gctgggctaa acccgtccag gtccgccgaa
     5341 gtgccgcgcg caagtcaagc tcccggcgag gcaaatccag gatgaatcag ggtccttgta
     5401 ttgcgtcggc aagatagggc ctaccctctt gcaagcggct cccgagcttt gaacaatcgt
     5461 ggaacagaat cctccgcgcg accgatccgg cacgccgtcg cgggggactc acgaggccat
     5521 cgagcgcgcg ctcgaccgca tcccttcccg cgagactgca cgagtgccga aggaaatgtc
     5581 gatcatgagg cgaatacgaa cgggtctggt actcttcagc gcggctggca tgctcggcgc
     5641 ggccgcactg gccgagacca aggtcgaaat cggaaatgtt catctgtgct gccccggttg
     5701 cgtcaaagcc gtcgactccg cgttggcggg cgtcgaaggg gtcacgggcg tctgcgacca
     5761 gaaggcc
//
LOCUS       DATAQL010000109         4698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1995468, whole genome shotgun sequence.
ACCESSION   DATAQL010000109 DATAQL010000000
VERSION     DATAQL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995468"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VHS97_01850"
     CDS             <1..988
                     /locus_tag="VHS97_01850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX3446963.1"
                     /translation="DRLEGQFDPNKAAVVIGSAFGGLDLLDAEQQRMIKRKSLAVSPY
                     LVPAMIINQAAGQIAQHLGLYGPSAAPANACASGGHAVVLGAMFLRSGDAELALCGAG
                     ESAFTPAVINGFATMKALLGKKPGDRSEHDPGQGSRPFSIDRAGFVMSEGAGALVLAT
                     ESAARRLGLELQAELVGWSTNSDGYHMAMPSRERIRRCLETALERSALNCDQVDYYNA
                     HGTSTSVNDRVETQVLRDVFGASVGRLPVSSIKGAIGHSLGAASAIEAAVAVRALRAQ
                     LIPPTINFMADPELELDYVPNHARPARLDVVVTASFGFGGTNNALVLRNTPS"
     gene            1133..2059
                     /locus_tag="VHS97_01855"
     CDS             1133..2059
                     /locus_tag="VHS97_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3446964.1"
                     /translation="MERRGLNFRKNFLRVFLPIFRALPLPVASRFVSGIGRIEYRWSK
                     SLRASFHNAVSSGQSMLECDWDVPTVSRELAGNHVLWRTRDLLLDGVPDERAKDMFRV
                     TGRDYIDAAIGLGRGCIVLASHFGAHLLPAHWLFRENFPVRFYMERPRHISRYMARHF
                     DTSGPLGQDKLFISRQGVPADSASSILRATRAIKAGMLLYLAGDVRWTGQLTESAQFL
                     GRTMRFSTTWVALAAMTEAPVVMVFCRMEPDGRYHLEFRKSFQIPKDVAARGEVGRWV
                     QHFMDTLEQQVRRYPANSNDYFFWEELSGLVA"
     gene            2395..3681
                     /locus_tag="VHS97_01860"
     CDS             2395..3681
                     /locus_tag="VHS97_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3446965.1"
                     /translation="MALPIRLSAAAKRTNPSSISHLMQTALENPGIVSLAAGFVDQQS
                     LPVDVVAQVASDLLGDPTEGRRSLQYGTTIGDLRLRSRLIDHLERSEGRSPGSFKEAI
                     SRTVVTTGSAQLIYLVCEAILDPGDIVLVESPTYFVFLGPIETRGARAIRVPIDDGGL
                     RLDKLDMILTHLERQGELERVKLIYTIPEHANPTGISLDADRRKPLLELARRWSKKHR
                     IFVLEDAAYRGLSFGVNEPPSLWSLDSNQETVILARTFSKTLSPGLKLGYGILPIGLR
                     EPILTLKGNHDFGTANLNQRLLERMLSDGSYDRHVARLTKLYRRKCGIFLEALDDHVG
                     SLDSEVRWTRPEGGLFVWMTVPEDLDTSFEGPLFPLCVREGVLYVPGDHAFATEPEPA
                     PKNHLRLTFGVPSELELVEGARRFAAALSACLHPVA"
     gene            3775..>4698
                     /locus_tag="VHS97_01865"
     CDS             3775..>4698
                     /locus_tag="VHS97_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX3446966.1"
                     /translation="MGILQRYVMGEVTRSFGLALLSMTAIFVLFMVAAQAKDIGLSPN
                     EILRLVPYVIPSTLPYTIPVSLLFAATVVYGRLAGDNEIIAVKTAGLSVWMVLYPTLT
                     LAAALSLWLLYVSGGWIPDCNHNAKLVLFKDVEDMFYKLLARDREFNHPKWPFLIKVR
                     DVQGMKMIDATFKHKVKGTNEFDAVIQAREAKLHFDLPTKLVTIFLEKSEIQKFDRDA
                     DVVLINNDVLKIPIPPDSQINTDKKLQENSNAEIVAELAKNRKLIATVRKRESIKAGF
                     AFASGRLESINWTDVNSAYRDHPDWIRRCDEL"
BASE COUNT          941 a         1377 c         1425 g          955 t
ORIGIN      
        1 tgaccggctc gagggtcagt ttgacccgaa caaggccgcg gtcgtcatcg gttcggcatt
       61 tggcggcctc gacctgctgg acgccgaaca gcagcggatg atcaagcgca agagcctggc
      121 ggtgagccct tatctcgttc cagccatgat catcaaccag gccgccgggc agatagccca
      181 gcacctcggg ctctacggcc cgagcgccgc gccagccaac gcgtgcgctt cgggcgggca
      241 cgcggtcgta ttgggcgcca tgttcctgcg atccggagac gctgagttgg ccctgtgcgg
      301 cgccggcgag agcgcgttca caccggccgt gatcaacggc ttcgccacga tgaaggcact
      361 cttgggcaag aagccgggcg atcgttcgga acatgaccca gggcagggca gccggccgtt
      421 cagcatagat cgcgccggat tcgtcatgtc ggagggagcc ggagcgctcg ttctcgcaac
      481 cgaatccgcg gcccgccggc tgggcctgga gcttcaagcc gagctagtgg gctggtcgac
      541 caattccgac ggttaccaca tggccatgcc ttctcgagag cgcatccggc gttgcctcga
      601 gaccgcgctc gaacgatcgg cgctcaattg cgaccaggtt gactattaca acgcccatgg
      661 tacgagcacc agtgtcaacg atcgtgtcga aacgcaggta ctccgcgacg ttttcggcgc
      721 gtctgtcggg cgtctgccag tcagctcgat taagggagca atcggccata gtctgggcgc
      781 ggcatctgcg atcgaggctg ccgttgcggt gcgagccttg cgcgcgcagc tcatcccgcc
      841 caccattaat ttcatggccg accccgaact ggagctcgat tatgtgccaa accacgcccg
      901 gccggcccgg ctcgacgtcg tcgtgaccgc gtcgttcgga ttcggaggca ccaacaacgc
      961 acttgttctg aggaacacgc catcatgacc gcgatcgcgc aatctttccg aaagaatcaa
     1021 aaaagggacc acaaaaaggg gtcagaatgc ttttagcccg cccttttttg tggttggccc
     1081 gttttacggc gcgtgcaaga tgcggtgaat atcgggtgag ggagagtcgg caatggagcg
     1141 acgcgggctg aacttccgca agaatttcct gagggtgttc ctgccgattt tccgggcgtt
     1201 gccgctgccg gtcgcctcgc ggttcgtatc agggatcggc aggattgaat atcgatggag
     1261 caagagcctg cgcgcgagct ttcacaatgc cgtaagctcc ggccaatcga tgctcgagtg
     1321 cgattgggac gtaccaacgg taagtcgcga actggccggc aaccacgttc tctggcgcac
     1381 gagagacctg ctcttggatg gcgtgcccga tgagcgcgcc aaggacatgt tcagggtgac
     1441 cgggcgcgat tatatcgatg ccgcgatcgg cctggggcgc gggtgtatcg tactggcgag
     1501 ccacttcggt gcgcatctcc tacccgcgca ttggctcttc cgcgagaatt tccccgttcg
     1561 attctacatg gaacgtcccc gccacatttc gcgttacatg gcgcgacatt ttgacaccag
     1621 tgggccgctg ggtcaggaca agctcttcat ctcgcgacag ggggtgcccg cggattcggc
     1681 cagctcgatc ctccgagcga cgcgggcgat caaggccggg atgctgcttt atctcgcggg
     1741 cgatgttcgc tggaccgggc aactcacgga atctgcccag tttttgggcc ggactatgcg
     1801 attctcgacg acctgggtcg ctttggcagc gatgaccgaa gcgcctgtcg tcatggtatt
     1861 ttgccgcatg gagccagacg gccgctacca cctcgagttt cgcaagtcat ttcagatacc
     1921 aaaggacgtg gctgcacgcg gggaagttgg gcgctgggtt cagcatttca tggatacgct
     1981 tgagcagcag gtccgccggt acccggccaa cagcaatgac tatttcttct gggaagagct
     2041 gagcggtttg gtcgcgtagt cagtgtttgc tccacttcga cgatacgccc gagccaggcg
     2101 atcggtggcg tggcgccgga cagccgcgat aggcgatcga gggaccgaac cccggccgtg
     2161 cgacgcgtgt cggttgataa ccacgcatct tcaaagatcg cgtgagggtg gtgttcatgg
     2221 agactcgttg gccggcggac cggaacgagt tagaatggaa aggccgagcc gatccacatg
     2281 ctcaaagcgt tggagtcggt cctgtctaac cgagcttgac accctcggcc aggtggatag
     2341 agtacgcaca aacacgcatt gtctcgcttc cccacgagct ggcaaatccc aacgatggca
     2401 cttccgatac gattgagcgc ggccgcgaaa cggacgaatc cctcctcgat cagccacttg
     2461 atgcagaccg cgctcgagaa tccgggaatt gtgtcgctgg ctgccggttt cgtggatcag
     2521 cagtcactgc ctgtcgatgt ggtggcgcag gtcgcctctg atctcctggg agatccgaca
     2581 gaaggacgtc ggtcccttca gtatggaaca acgatcggcg acctgcggct acggtctcgg
     2641 ctcatcgatc atctggaacg gagcgaaggc cgctccccag gttctttcaa ggaggccata
     2701 tcacgaacgg tcgtcacaac tggttcggcc caattgatct acctggtttg cgaggcgatt
     2761 ctggatcccg gcgacatcgt cttggtggaa tcgccgacgt actttgtctt tttgggaccg
     2821 atcgagacgc gaggggcacg cgcaatccgg gtgccgattg acgatggcgg gctccgcctc
     2881 gacaagctcg acatgattct gacccacctg gaaaggcagg gtgagctcga acgggtcaag
     2941 ctgatctaca cgatccccga gcatgccaat ccgacgggta ttagcctcga tgctgatcgt
     3001 cgcaagccgc ttttggaact ggcccgacga tggtccaaga agcatcgaat cttcgtgctg
     3061 gaagacgctg cttatcgcgg tttgtcattc ggagtcaacg agccgccgag tctttggagc
     3121 ctcgacagta accaggagac ggtcatcctg gcccggactt tcagcaaaac cctgagcccg
     3181 ggactcaagc tcggctacgg aattttaccg atcgggctcc gcgagccgat cttgacgttg
     3241 aaaggtaatc acgacttcgg cacggcgaat ctgaatcagc ggctgctgga acgaatgcta
     3301 tcggacggaa gctacgaccg gcatgtcgcg cgtttgacca agctgtaccg gcgaaagtgc
     3361 gggatttttc tcgaagcact cgacgaccac gtgggatcgc tcgattccga agtacgctgg
     3421 acgcgtcccg aaggtgggct tttcgtctgg atgactgttc ctgaagacct cgacacgagt
     3481 tttgagggcc cgctgtttcc tctgtgcgtg cgggagggcg tcttgtatgt ccccggagac
     3541 catgcattcg cgaccgagcc cgagccagcg cccaagaacc atctccgcct cacgttcggc
     3601 gtgccgagcg agctggagct ggtcgaggga gcacgccgat tcgcggcggc tttgagtgca
     3661 tgcttgcacc cggtagcttg atggcgactc aaggaagatc gagtccgttt tgataacccg
     3721 ccgtgcggga attactagcc cgcggcaagg acgcttacac gagggctatc gaaattgggg
     3781 atacttcagc gctacgtgat gggtgaagtc acgcgctcct tcgggctcgc gctcttgtcc
     3841 atgaccgcga tcttcgtgct gtttatggtc gcggctcagg ccaaggatat cgggttgtca
     3901 cccaatgaga tccttcgtct cgtaccctac gtcatcccca gcacattgcc atacaccata
     3961 ccggtttccc tcttgttcgc ggccacggtc gtctacggcc ggctagcggg cgacaacgag
     4021 atcatcgccg tcaaaaccgc cggcttgagc gtgtggatgg tgttgtaccc gacgctgact
     4081 ctggcggcgg cattgagtct gtggctcctt tatgtgagcg gtggctggat ccccgactgc
     4141 aaccacaacg caaaactggt cctcttcaag gacgtcgagg acatgttcta caaactgctg
     4201 gcgcgcgatc gcgaattcaa ccatcccaaa tggccgttcc ttatcaaggt tcgcgacgtg
     4261 cagggcatga agatgatcga cgccaccttc aagcataaag tcaaggggac caacgagttc
     4321 gacgccgtga tccaggcaag ggaggccaag cttcactttg atctgcccac caaacttgtg
     4381 acaatttttc tcgaaaagtc tgagattcag aaatttgacc gcgacgccga cgtcgtgctg
     4441 atcaacaacg acgtactcaa gattccgatt cctcccgaca gtcagatcaa taccgacaag
     4501 aagttgcaag agaatagcaa tgccgagatc gtggccgaac tcgccaagaa tcggaagctg
     4561 atcgcgaccg ttcgaaaacg cgaatcgatc aaggcagggt tcgcctttgc ctcgggtcgt
     4621 ctcgaatcga tcaactggac cgacgtgaat tcggcgtatc gagaccaccc cgactggatc
     4681 cggagatgcg acgaactt
//
LOCUS       DATAQL010000110         5117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1232471, whole genome shotgun sequence.
ACCESSION   DATAQL010000110 DATAQL010000000
VERSION     DATAQL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232471"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VHS97_01870"
     CDS             <1..294
                     /locus_tag="VHS97_01870"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3446967.1"
                     /translation="LLPHALRFNRDAAGPRMADLAGRLGLGRTGDAAGHLVTLTELLL
                     ARMPLPRRLGELEGLERDRIAEYSRLAMLDHCHRTNPRVCTPADMEDLLDRAW"
     gene            444..1538
                     /locus_tag="VHS97_01875"
     CDS             444..1538
                     /locus_tag="VHS97_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX3446968.1"
                     /translation="MRTNLSGLPVARADLATGYDAILVVGFGGPECREDVLPFLENVT
                     TGRNIPRERLLEVAEHYYHFDGVSPINAQVRELIAALRIELDGHGMSLPIFWGNRNWH
                     PLLGDTLAEMTRGGVKNALAVVLAAYSSYSSCRQYREDIARAQSAVGPSAPHIDKVRV
                     YYNHPDFIAANADRIRDAWGRFASERRDSVHVAFTAHSIPIAMARNCDYERQLSAACR
                     LVAEELGIPQERWALVYQSKSGRPGDPWLEPDILQYLRDLNARGTDSVVIHPIGFLSD
                     HMEVLFDLDEEARLLCQGLGMEMVRARTVGTHPLFVSLLRELIAERIGFLPEPVRRAA
                     GDDGPSHDVCPETCCLPAPRHSSPTAVQPG"
     gene            1717..2883
                     /locus_tag="VHS97_01880"
     CDS             1717..2883
                     /locus_tag="VHS97_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX3446969.1"
                     /translation="MASRRLLLVEDSSTMRRMLSTMLKEEGFEVETANDGLQGLAKAR
                     LDPRPELILTDYEMPELDGAGLCQALKADKELRSIPVMMLTTLGEAQNKIAGLTAGAD
                     AYIEKPKGKDDFRVLCAQIQAHLRIADLGIELANRNRLLESAHNKLKFELELARKVQE
                     ALMPRPPKPRGMLRLAVRYSPANQLGGDVYDFYRLDNNRLGILVADVSGHGVNSAMLS
                     GMVKAMAAPLSLAVLEPGELLAGLDVAGEQYFPEGYFCTGFYLIADEETGLVRYAGVG
                     HPPAMIVGPQGPRTLPSNPGMLGIGMVDGTAGATDRIEPGESLVIYTDGLTDAMDPSD
                     VLFGEDRLRTLLQSHYGADPAEILNQVASALDHHTSPGRPADDINIIVLQHLAK"
     gene            3047..3343
                     /locus_tag="VHS97_01885"
     CDS             3047..3343
                     /locus_tag="VHS97_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3446970.1"
                     /translation="MAVEQTEYKTIRLTGPVTLYEVSAVRESLRKALAEGKPLRLDLS
                     DSGPWDLAGVQLLVSCVKAARDRDRQARLVSVPKVCVEVAERCGLAEWLSSYQE"
     gene            3481..3846
                     /locus_tag="VHS97_01890"
     CDS             3481..3846
                     /locus_tag="VHS97_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3446971.1"
                     /translation="MSKTVLHADDSASVRRWVAEQLGELDLKVISVADGEAALNVLRE
                     APCDLLLTDLEMPNLDGLSLVSAVRDLPMHRFLPVLILSCRQPTEFQPEHRQGVTGWL
                     IKGIEPEQLRRWVKRVLPR"
     gene            3879..>5117
                     /locus_tag="VHS97_01895"
     CDS             3879..>5117
                     /locus_tag="VHS97_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX3446972.1"
                     /translation="MSSGADDRFREMFYEEARELLISLEEGLMDLERRQSDRAHLDKT
                     FRAAHSLKGAAAMVGLAAIAEFTHRIEAVLERIRAGTLPVDSDIITTLLESRDHLAAM
                     VEAEAARSPISPSGELSHRLISLLGGPSQPKTSEPPGSGSSGSSPPPPNKPPENAPVS
                     QAPVTSSPPALPAQARDDPEPNSKVKKPSTTPGGETKKKPRAPRGKTRKMDQAPGPPP
                     ASGKDEIDGESGGSNLTIYQIKLTPGRDVLRRGINPLGVLDELRELGETSIVTDPETV
                     PALDQIDPERCYLNWTITVKSAAEPERLFEAFLFFAEDSTIVIERQGPDGNWVPMRLG
                     EAGTPDGFPAAKSATAANGDNLTLPAVAAPVAHEVPLAPAATNGHAPSPALRVPSQPV
                     AMNGGAATSPVLPRVHARIRV"
BASE COUNT          989 a         1523 c         1592 g         1013 t
ORIGIN      
        1 ctccttcctc atgcgcttcg gttcaatcgc gatgccgccg gaccgcggat ggccgacctc
       61 gcggggcggt tgggcttggg tcgcaccggg gatgcggcgg ggcacttagt tacgctcact
      121 gagctgttgc tggcacgcat gccgttgccg cgccggctcg gagagctcga agggttagag
      181 cgcgaccgga tcgccgagta ttcacggctt gccatgctcg accactgcca tcgcaccaac
      241 ccgcgcgttt gtacgccggc cgacatggaa gacctgcttg atcgcgcctg gtgatttttc
      301 gtaacggcgc cggtgtcatc gccggccacg atcctctcag gttatgcctg gctgaaacca
      361 gacccttgag actgatttcg ttttggctac aactatggtg tctaagacgc ccttgattct
      421 gtcacgagat ggtttctcac gtcatgcgca ccaacctgag cggtctgccg gtcgctcgcg
      481 ccgatctggc gaccggctac gacgcgatcc ttgtggtagg tttcggcggc cccgagtgcc
      541 gtgaggatgt tttgcccttc ctcgaaaacg tgacgacggg tcgcaacatt cctcgagaac
      601 ggctcttgga ggtggcggag cattattatc atttcgacgg agtgagcccg atcaacgctc
      661 aggtccgcga attgatcgca gcgttgcgaa tcgagctcga cgggcacggg atgtcgttgc
      721 cgatcttctg gggaaatcga aactggcacc cgctcttggg tgacacgcta gcggagatga
      781 cgcgcggtgg agtcaagaac gctttggccg tcgtgctggc agcttacagc tcgtactcca
      841 gttgtcgaca gtacagggaa gacatcgccc gcgcccaatc cgcagtcggt cccagcgcgc
      901 ctcacatcga taaagtaagg gtttattaca accacccaga ctttattgcg gccaacgccg
      961 accgaattcg cgacgcctgg ggccgattcg cgtccgagcg ccgcgactcc gtgcatgttg
     1021 ccttcaccgc gcacagcatt ccaatcgcga tggcgcggaa ctgtgattac gagcggcaat
     1081 tgagcgcggc ctgtcgattg gtagccgaag agctcggtat tccgcaagag cggtgggcct
     1141 tggtctacca gagcaagagc ggtcgccccg gcgatccgtg gctcgagccg gacatccttc
     1201 aataccttag agacttgaat gcccgcggga ctgactccgt cgtgattcat cccatcggat
     1261 ttttgtccga tcacatggag gttcttttcg acctggatga agaagcccga ctgctctgcc
     1321 aagggctcgg tatggagatg gtccgagcgc gaacggtcgg cactcatccg ctttttgtga
     1381 gtctactccg agagctaatt gccgaacgga tcggatttct tccagaaccc gtgcggcgcg
     1441 ccgccggcga cgatggaccc agccacgatg tctgccccga aacttgctgc ctcccggccc
     1501 ctcgccattc ctcgccaaca gcagtgcagc caggctgagc tagggcgctg atttcggcca
     1561 gtagggcacc acacttgcgc tgacgtgccc tagacattct ggccgccccg cttttatcgt
     1621 ggtactgaac gtacacgtgc caaatctatg ctctggccgg aatgatcggg ctggggataa
     1681 gttcgattac agaatcagaa tgaggagaga gggctgatgg cgtcgcgccg cctcttgctt
     1741 gtcgaagaca gttccaccat gcggcgaatg ctctcgacca tgctgaagga ggaagggttt
     1801 gaggtcgaaa ccgccaatga cggcctgcag gggctcgcca aggcccgact tgatcctcga
     1861 cctgagctga tcctgacgga ttacgagatg ccggagctcg acggtgccgg gctttgccag
     1921 gcgctcaagg ctgataagga gctgcgatcg attcccgtca tgatgctcac gacgctcggt
     1981 gaggctcaaa acaagatcgc cggtctgact gccggagccg acgcctatat cgaaaagccc
     2041 aagggcaagg atgacttccg ggtactgtgt gcgcaaatcc aggcgcactt gcgaattgcc
     2101 gacctgggta ttgaactcgc caatcgcaac cggctgctgg aatccgcgca caacaagctg
     2161 aaattcgagc tcgaactggc aagaaaagtc caggaagccc tgatgccgcg cccgccgaag
     2221 cctcgaggca tgctgcggtt ggcggtgcgg tactcgccgg caaatcaatt gggcggagac
     2281 gtctacgatt tttaccggct tgacaacaat cgcttgggga tcctggttgc ggatgtgtcg
     2341 ggccacgggg tcaattcggc gatgctgtcg gggatggtca aggcgatggc cgcgccgctg
     2401 tcgctcgccg ttttggagcc gggtgaatta ctagctggct tggacgtggc cggagaacag
     2461 tattttcccg aaggttactt ttgcacgggc ttttacttga tcgcagatga ggagacgggt
     2521 ctggttcgct acgcaggtgt agggcatccg ccggcgatga ttgtcgggcc gcaggggcca
     2581 cgcacgctgc cctcgaatcc cggtatgctg ggtatcggta tggtcgatgg cacagcaggt
     2641 gccaccgatc gcatcgagcc gggggagtct cttgtaatct atacagacgg tttgaccgat
     2701 gccatggatc catccgacgt acttttcgga gaagaccggc tgaggaccct gctgcaatct
     2761 cattatggcg cggatccggc cgagatcttg aaccaggtcg ccagcgcctt ggaccaccac
     2821 acgtcgccgg gccgccccgc cgacgacatc aacatcatcg ttctccagca tctcgccaag
     2881 tgaatgctcc ccaagccacc ggcggcatgt taaactatac gagggtttgt ggcccgatca
     2941 gggcggcgag ccttccgttc cagcttcctc ttaatcagtc agtttcagga cggtttggga
     3001 tccactcaac tcccttctta gcttggggtt agcgtgggat cgcaccatgg cggtcgaaca
     3061 aacagagtat aagacaatcc ggctgaccgg gccggtcacg ctctacgagg tgtcggcggt
     3121 ccgggaatct ttgcgcaagg cgctggcgga aggaaagcca cttcgcctcg atttgagcga
     3181 ctcgggaccg tgggatctgg ccggcgtcca gcttcttgtt tcatgcgtga aagccgctcg
     3241 ggatcgagat cggcaggcac ggctggttag tgtccccaag gtttgcgtcg aggtcgccga
     3301 acgatgcggg ttggcggaat ggctgagttc gtatcaggaa taaagaaaac ggggggagta
     3361 tatcccaagg aaaaatcacg agtggtggct cgggccgctc aggctggatg agcacgtccc
     3421 gcggaatcgt tcgacgggcc tcatcttgac cgcgcacggc cgtggaacac ggttaaaatc
     3481 atgagcaaaa ccgtcctgca tgccgacgac agcgcttcgg tgcgacgctg ggtggccgag
     3541 caacttggcg aactggacct caaggtcatc tcggttgccg atggtgaagc cgcgctcaac
     3601 gtgttgcgcg aagcgccctg cgatctgttg ctgactgatc ttgagatgcc gaacctcgac
     3661 ggattatcgc tggtatccgc ggttcgcgac ctgccaatgc atcgtttctt gccggtattg
     3721 attctgtcat gccggcagcc caccgaattc cagccggaac atcggcaggg tgtgacagga
     3781 tggctcatca agggcatcga acccgagcaa ctgcggcggt gggtcaaacg ggtattgccg
     3841 cggtgagcag ttcatctttc tggtgaggga caacggattt gagcagcggt gcggacgacc
     3901 gattccggga gatgttttac gaggaagcac gcgagctttt gatcagtctc gaagagggac
     3961 tgatggacct tgagcggcgt cagagcgacc gcgcgcatct cgacaaaacc ttccgggcgg
     4021 cgcacagctt gaagggagcc gcggccatgg ttgggctcgc ggcgatcgcc gagttcaccc
     4081 atcgaatcga ggctgtcctt gagcggatcc gggccggcac gctgccggtc gattccgata
     4141 tcatcacgac gttgctcgag tcgcgcgatc atctggcggc catggtagag gccgaggcgg
     4201 cacggtcacc catctccccc tcgggcgagc tgtcgcaccg cctgatttcc ctcttggggg
     4261 gaccttcgca gcctaaaact agcgaaccgc ccggcagcgg atcgtctggc tcatctccgc
     4321 cgccacccaa taagccaccg gaaaacgcgc cagtttcaca ggctccggtg acttcttcac
     4381 ctcccgcttt gccggcgcaa gcccgcgacg accccgagcc gaattccaag gtgaaaaaac
     4441 cctccacgac gcccggtgga gaaaccaaga aaaagccacg tgcaccgcgg ggaaagaccc
     4501 gcaagatgga ccaagcgcca ggtccgccgc ctgcgtccgg caaggatgaa atcgacgggg
     4561 aatcgggcgg ctcgaatttg acgatctacc agatcaagct cacccccggc cgggatgtcc
     4621 tgcggcgtgg gatcaatccc ctcggagtct tggacgagct gcgcgagctg ggcgaaacat
     4681 cgatcgtcac cgaccccgaa acggttccgg ccctcgacca gattgatccg gaacgctgct
     4741 accttaactg gacgatcacg gtcaagtcgg cggcggaacc ggaacgcctg ttcgaggcat
     4801 tccttttctt tgccgaagac agcacaatcg tgatcgagcg ccagggcccg gatggtaact
     4861 gggtgccgat gcggctcggc gaagcgggga cgccggatgg gttcccggcg gcgaagtcag
     4921 ccacggccgc gaatggcgat aacctgacgc tcccggccgt agccgcgcct gtggcgcacg
     4981 aagtaccttt ggctccggcc gccaccaatg gccatgcgcc gtcgccggcc ctgcgggttc
     5041 cttcgcagcc agttgccatg aacggtgggg cggcgacttc cccggtgctt ccccgtgtgc
     5101 atgccaggat ccgggtc
//
LOCUS       DATAQL010000111         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3064708, whole genome shotgun sequence.
ACCESSION   DATAQL010000111 DATAQL010000000
VERSION     DATAQL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3064708"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VHS97_01900"
     CDS             complement(<1..274)
                     /locus_tag="VHS97_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446973.1"
                     /translation="MHETWPSWNAECARWKEPDVLQLDLVAATVFFPTTCALLISQEV
                     CRQISYAWLSWDSSEHFVKRDGRALAHNTLEEMRILAVKRMAAGEHP"
     gene            549..1451
                     /locus_tag="VHS97_01905"
     CDS             549..1451
                     /locus_tag="VHS97_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3446974.1"
                     /translation="MEVGRLRALLELARCGTMAAAAEALFLTPSAVSQQIAQLEEEAG
                     VKLTERIGRGVRLTPAGHALVGHAERVMVVLDEARSELAELRREIAGELRVAAFSSIA
                     SAVLPDTVKALQHAYPRLEIVLEEFDPIEGLTALRSWRTDIALIDDLSVVVGDSQESV
                     GVVPVAEDALYVAVAADHALSKRLSVSVADLRHETWAIESTWSGFGDVITNLCRRAGY
                     EPRINAKCRGFEMVVAMVASGCSVSMVPGLRLVRPPAGVTWVKLRPEVRRKVYVAYRR
                     GERDHPTIKVFVEEIVRTASRLLG"
BASE COUNT          344 a          493 c          547 g          351 t
ORIGIN      
        1 cagggtgctc acccgccgcc atgcgtttca ctgcgagtat gcgcatttcc tcaagcgtgt
       61 tatgagccaa tgcccggcca tctctcttca cgaaatgctc cgacgagtcc caagataacc
      121 acgcatagga tatttgtcgg caaacttcct gactgatgag taatgcgcag gtcgtgggga
      181 aaaacacggt ggccgcgacc agatccagct gtaaaacgtc aggctctttc cagcgagcgc
      241 attcggcgtt ccaactcggc caggtctcgt gcacccacaa ggtactcgtc gttacggtgt
      301 tgttcgacgt gctcgaacca gtggctaatc tgcgtgaaaa gaaaggcaag catgacaatc
      361 tccaagaggt tgctcgtagg ggacgccgcc gtccgaccgc ggcgctgcgc ctctgtctcg
      421 ctgttgttct ccggcgccga tctgcaactc aatggtaggc aatggatatg ctcacgtata
      481 cttatctttc ttgatctaat acattagaga ttctgtttaa tatccaagtc tcctcgaggg
      541 taaagatcat ggaagtcggg cgtcttcggg cgctgctgga gctggcgagg tgtggcacga
      601 tggcggcagc cgccgaggcc ctgttcctga caccgtcggc agtttcacag cagatcgcac
      661 aactggaaga ggaggccggt gtcaagctca cggagcgcat tggccgcggg gtgaggctta
      721 cgcccgccgg ccatgcgcta gtgggccacg cggaacgagt catggttgtg ctggacgagg
      781 ctcgctcgga attggcagag ctacggcgcg agatagccgg cgagctccgc gtcgcggcgt
      841 tttcgtcgat cgcgtcggcg gtgctacccg atacagtcaa ggcgttgcag cacgcctatc
      901 cgcgcttgga gattgttctt gaagaattcg atccgattga ggggcttacg gcgttgaggt
      961 cctggcgaac ggacatcgcc ctgattgacg acctctcggt cgtggtgggc gatagtcaag
     1021 aaagcgtagg ggtggtaccg gtcgcagagg atgcgctcta cgtcgcagtc gcggccgatc
     1081 atgcgctgag caaaaggctg tcagtgagcg tagccgatct ccggcatgaa acctgggcca
     1141 tcgaatcgac gtggagtggc tttggcgacg tcatcaccaa tttatgccgc cgagccgggt
     1201 acgagcccag gatcaacgcc aagtgccgtg gattcgagat ggtagtagcg atggtggcct
     1261 ccggctgctc ggtgtcaatg gtccccggac tcaggctggt gaggccaccg gccggcgtga
     1321 catgggtcaa gctgaggccc gaggttcgcc gaaaggtcta cgtcgcttat cgccgcggcg
     1381 agcgcgacca cccgacaatc aaggtgttcg tcgaggaaat cgtccggacg gcatcgcggc
     1441 tcctcggtta gccgaatcga cgacgctcat acgccaactc gcaggcgcaa gataccggtt
     1501 attgcagact ggcaaaattt gggacggccg aatggccggt atcgccgatg acgggatgca
     1561 catcatcggt ggtcgaagga ctgttgacgg agtgaatcgg tcgcacgagt gtccaaacgg
     1621 cggccacgac gaatgactcg tcctggccgg acctggtccc acggccagcc tgcatgactc
     1681 gggtgaaccc gcttgctgcg acgggccgcc ctcgacccgt tactgcccct cagtt
//
LOCUS       DATAQL010000112         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2911892, whole genome shotgun sequence.
ACCESSION   DATAQL010000112 DATAQL010000000
VERSION     DATAQL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2911892"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VHS97_01910"
     CDS             <1..1216
                     /locus_tag="VHS97_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3446975.1"
                     /translation="GLTALGLIFGVAAVISMLAVGEGASRDAQLRIEELGATNIILRS
                     VKPSEDAQAASGRSAPLILRYGLKYADYERIVETVPTIKQVLPVREIRKQIRSVESAF
                     DGRIVGTTHEYAEFNHLKVARGRFLERSDDTLYRNYAVLAHATAEALFPYQDPIGETI
                     ALGSDAYKIVGVTGERASTAASGGGFAGQDYNRDVYIPLNTCRLRFGERIIDFRSGRF
                     SAEETELTQLTIQVQSTYEVTPTANVLTATYEPYHPEKDVQMTVPYDLLEQARRSARQ
                     FSIILGTIASISLLVGGIGIMNIMLATVTERTREIGIRRALGAKRRDIIQHFLIECVL
                     LSGVGGLAGVLLGVWIPRIIDYFVPDQKTIITAQSVVLAFGISVGVGILFGIYPARRA
                     SLMDPIDALRHE"
     gene            1607..>2155
                     /locus_tag="VHS97_01915"
     CDS             1607..>2155
                     /locus_tag="VHS97_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446976.1"
                     /translation="MRTGGIRGEEILEIGKWLFWDNRGSLNFYPHRPIRDVSSPGDGG
                     PRTSFGESPDDPDQPALLVRRLQSNAAGCQWMLDRWAELGTLVECELAWLSPDKLKAI
                     RLMGMQPIDAIDDLDVARIFLACFVIRGSTGDPFHEIIYELFTREEVVFRNQVAARLL
                     TAFMPKDAADARAVLAAIVANAR"
BASE COUNT          413 a          701 c          619 g          422 t
ORIGIN      
        1 gggcctgacg gccctgggtc ttatcttcgg cgtggcggcg gtgatctcga tgcttgcggt
       61 gggagaaggg gccagccgcg atgcccagct ccgcatcgag gagctcggcg cgacgaacat
      121 catacttcgc tcggtcaagc cgtcggaaga cgcccaggcc gcttccggcc ggtcggcgcc
      181 acttatcctc cgctacggac tcaagtacgc cgactacgag cgcatcgtcg aaaccgttcc
      241 gactataaag caggtgttgc cggtccgcga gatccgcaaa cagatccgct ctgtcgaaag
      301 cgccttcgac ggccgcatcg tcggcacgac tcacgagtac gccgaattca atcacctcaa
      361 ggtcgccagg ggtcggtttc tcgagcggtc cgatgacacg ctctaccgca actacgccgt
      421 gctcgcgcac gcgacggccg aggccctttt cccctaccag gaccccatcg gcgagacgat
      481 cgctctgggg agtgacgcct acaagatcgt cggcgtaact ggtgagcgcg ccagcaccgc
      541 tgcgtcgggc ggaggcttcg ctgggcagga ctacaatcgt gatgtgtaca ttcctctgaa
      601 cacctgccgg cttcgattcg gcgagcggat catcgacttc cgctcggggc gcttcagcgc
      661 cgaagaaacg gaactcactc agctcacgat ccaggttcaa tcgacctacg aggtgacccc
      721 gacggccaac gtactgaccg cgacttacga gccctaccat ccggaaaaag atgtgcagat
      781 gacagtgccg tacgaccttc tggagcaggc ccggcgctcg gcccgtcagt tcagcatcat
      841 cctcgggacg atcgcctcaa tctcgctctt ggtcggcggc atcggcatca tgaacataat
      901 gctggccact gtgaccgagc gaacccgtga gatcggaatt cgccgcgcgc tgggcgccaa
      961 gcggcgcgac atcatccagc attttctgat cgagtgcgtg ctcctctccg gggtcggcgg
     1021 cctggccggc gtgctcttgg gcgtttggat tccgcggata atcgattact tcgtgccgga
     1081 tcagaagacg attatcacgg cgcaatcggt ggtcctggca ttcggcatct ctgttggcgt
     1141 cggcatcctg ttcggcatct acccggcccg ccgcgcgtcc ttgatggatc cgatcgacgc
     1201 cctgcgccac gaataggggt ttgcacgccc aatgctcgac cagaagtaca cgcacccgtt
     1261 gatctccgtg tgcttcgccg gtgtcggaca ggcacggcag gtcgctcgcc gcggaaatcc
     1321 caaccttctg gttgtgccgc cattacactg acccgtcaat tcgcccgccc gcgaaacaaa
     1381 gtccctcccg gttcgtccaa tcgtgccaac aattcaagat attccttgcg ttacagcaac
     1441 actcttatcc gttccaatag caaagtctcc tgttatggac gatcttcctt attatagccg
     1501 ccttacccaa acgacaggct tttgttggtc accctcttga cgcccaaaaa ctgcgtaact
     1561 atccaacggg aggagcaaaa tattatggcc accgcagctc aaatcaatgc gaacaggcgg
     1621 aattcgtgga gaggagatcc tcgaaatcgg caagtggctg ttctgggaca atcgcggctc
     1681 gctcaacttt tacccgcacc ggccgatccg cgatgtgagt tcacccgggg acggcggccc
     1741 gcgcacgtca ttcggtgaga gtcccgacga ccccgatcag cctgctctgc tcgtgcggcg
     1801 cctgcaatca aacgccgccg gctgccagtg gatgctcgac cgctgggccg agctcgggac
     1861 cttggtggaa tgcgagctcg cgtggctctc gcccgacaaa cttaaggcca tccgccttat
     1921 gggcatgcag cccatcgacg ccatcgacga tctggacgtc gcccggatct tcctcgcctg
     1981 cttcgtgatc cgaggcagca ctggcgaccc gttccacgag atcatctacg agttgttcac
     2041 gcgtgaggaa gtggtgttcc gcaaccaggt ggccgcccga ctgctgacgg catttatgcc
     2101 caaagacgcg gccgacgcac gcgcagttct ggcggcgatt gtggccaatg cgaga
//
LOCUS       DATAQL010000113         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_164062, whole genome shotgun sequence.
ACCESSION   DATAQL010000113 DATAQL010000000
VERSION     DATAQL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164062"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VHS97_01920"
     CDS             <1..186
                     /locus_tag="VHS97_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446977.1"
                     /translation="MVAGFGMAIGAIQASHYLSWRQLCRTIIATADTSHIPPSPPVDI
                     MANGGLLGWWRRRQKTA"
     gene            complement(573..797)
                     /locus_tag="VHS97_01925"
     CDS             complement(573..797)
                     /locus_tag="VHS97_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446978.1"
                     /translation="MPDPKDDPKSSEWRPYMFARDEWHCRICGDRFLYSEMVRKHIAN
                     EHNETMPPSMYEYFHGAVWQRKKLISPSEW"
BASE COUNT          422 a          641 c          611 g          447 t
ORIGIN      
        1 atggttgcgg gctttggcat ggcgatcggc gcaatccagg cttcgcatta cttgtcatgg
       61 cggcagttgt gtaggacgat catcgccact gccgacactt ctcacatacc accgtccccg
      121 ccggtagata ttatggcgaa cggcggcctg ctcggctggt ggcgacgacg acagaagacc
      181 gcctgagccg gcgcaacacg tcgtttgggg cggccagtgt gggatcccac gggttaagat
      241 cttgttaagg tttctcaatc attaatgggg gtgtccaacc atcttctgga ctttgacacc
      301 tccctgtttc gcacttcgag ccgccagctc cggcggctct tttttcgtcg gcctgtccca
      361 tttttccttt cttagttcag gccgcaagcg gcgcgcgcct aaattcagca gccgttgccc
      421 ggcgttgcga gacggacagc cgccgcgatt agatcacacg aacggcactc atgactgcct
      481 cgttgagact ttgcgaacgc tgtcagccgg aacagcgagc gtttcgacgc ggccgtcgaa
      541 caggaccgcg ataaactcgt ctctaagctc gcttaccact cagaggggct gatcagtttc
      601 tttcgctgcc aaactgcgcc gtggaagtac tcgtacatgc tcggcggcat ggtctcgttg
      661 tgctcattgg caatgtgttt gcgtaccatc tccgagtaca ggaagcggtc accgcaaatg
      721 cggcaatgcc attcgtcgcg ggcgaacatg tacggacgcc actcggagct tttcgggtcg
      781 tctttgggat cgggcatttt aacaagtcct gaatctgatg ctgggcccgc tcgcgtcggc
      841 tgcgatctga aacgccatcg tttttcgaag cactaagtcg tttgtcccac gttcgcgcag
      901 ccaattgact cggccagagc cgccaaagcc caccatggtg tccccgcgct tccaaccgcc
      961 ggagcgaata aaccagccgc ggcggatcgg ttttggctaa aaggatcagg cagactggaa
     1021 ttcattttat cttaacgtcg aagatcccaa accggcgacc aacctcaacg acctagtcgg
     1081 tagggccatc attttggcgg tcgcctttta cttggtggcc gtcgtcgcga ctgtatgcgt
     1141 aacggacgcc ccgtaagaaa gtgggcgctt gacctgcttt gtcgcccggt ccagccatga
     1201 agagctgcct catctactac aacgaggtct cgccaaggcg ggcgggcatt cacccctttc
     1261 gagcaaaagg ccccgccgtg aatcgacggg gccagagtca tgcaattgtt cggactttcg
     1321 atgcggaaga gaggccgctg cttccgcagc acggcaattc gggacgcggg acatagttcc
     1381 cgatgccggc gcgcagcttc gccgctcacc ggaagtcaca cccgtctctg gcacgcaaac
     1441 caccgcttcc aaaattcgac gactggtttc agcagataaa gccaggctaa atgggcacac
     1501 gggcggcttg cgtgatggga atccccggcg gcttttgcgg cattacgccg ccggggaccc
     1561 ggacggtacc cggaccgact gtccgcaggc cgcccacctg attagacatc ccacagtcaa
     1621 gttcagttca tcagcccgcc catgaaagtg ctatgccgaa catcgcttcg tctgatgtga
     1681 tggccatcac gaaatggtcg agagcgaccg cttgaggttc ccgagactgc ggacgacgac
     1741 cacgacgacg ggtgagccga tacggcacag atgaccaaac ccgtcatctg gcgcaggcaa
     1801 gttgatggat acacatcaac tagaccgaga tgaatcgtga ccacggtttc acagacgctc
     1861 atcgcgagga cgctctggat tttctcgagt agtccttctg gcggcgtatg gcttatatgc
     1921 aatagacaca acgctccgcc taacggggtg acgctccacc ccgtcccaga tcgaatcgct
     1981 gggcggggtg gtaacgctgc gctcagcgag cgtgctcgga tgcgtctcag ccgtgccagt
     2041 gatgctccgc catgtgccga cgtgctgcga agcgcatggc gatggcgaaa cggcgcttct
     2101 gcgcctcgtc gaggctctgg t
//
LOCUS       DATAQL010000114         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2606953, whole genome shotgun sequence.
ACCESSION   DATAQL010000114 DATAQL010000000
VERSION     DATAQL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606953"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VHS97_01930"
     CDS             <1..477
                     /locus_tag="VHS97_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446979.1"
                     /translation="KVGKWTAAELEHAAEHWRRQFRGEAPVKNDNDITEADIAAHNLI
                     LWGDPGSNSLVAKVITKLPLTWDKENVRVGSATYGAGHHVPVLIYPNPLNHKRYVVLN
                     SGFTFREYDYLSNARQVPKLPDFAVVDIDVPVSARAPGGVATAGFFTEEWGLPATD"
     gene            553..1437
                     /locus_tag="VHS97_01935"
     CDS             553..1437
                     /locus_tag="VHS97_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX3446980.1"
                     /translation="MPSSGLPVIFYFMKQLLRELSNALFIILGILSAGMGIHGFLISS
                     HFIDGGVTGISMLLANVLGIPLPILILAINLPFIGLGYRQIGRAFAIRSALAISGLSV
                     CLATIHYPDVTPDKLLTAVFGGFFIGAGMGLAMRGGAVLDGTDIAALLISKRSHLFRV
                     GDVILGLNVLIFLTAEFFLGVEPALYSILTYLSATKTLDFLLHGLEEYTAITIVSERS
                     AAIREALTVGLGRGVTVYKGRGGMSGADQEILLCVVTRLEIGKVLNIAHDLDDSSFIT
                     IHPLAEVKGGVLKKTAHH"
     gene            1464..>3754
                     /locus_tag="VHS97_01940"
     CDS             1464..>3754
                     /locus_tag="VHS97_01940"
                     /inference="COORDINATES: protein motif:HMM:NF028477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HEX3446981.1"
                     /translation="MKMLNKQAIVGVAAVLLTTASVHAEPPGEYPTFRPPAVPLVAHN
                     PYLSIWSMADRLTDDTTRHWTRREHPLVSLIRIDGKAYRLMGTQPKDVPAMKQVGVRV
                     LPTRSIYDYDDGHLHVTLTFLTPALPEDLDVLARPLTYLTWEVRSVDGITHVVSIYDS
                     TSGHLAVNAPSQKVEWSRPACGGLTALRVGTVDQTLLQPAGDDTRIDWGYAYVAAPAS
                     QSQGAIGASAALLDHFVRLGALPKEDDTRKPRAADDDPPTLAIAFDLGSVGSTAVPRH
                     MMIAYDEIFAIKFLGQKLRPYWRRDGATADVLLTSAERDYADLVRRCAAFDAEMMADL
                     ASAGGARYAQIAALAYRQTLAGCGLAADANKQPLFFPKENSSNGCVATVDVIYPAAPQ
                     FLLMGPAYAKALVAPPLVYSTSPRWKFTFAPHDVGTYPQANGQVYGGGESSTNEADMM
                     PVEESANMILLCAAIAKMEGTAEFSSTWWPQLTRWEAYLEKYGEDPENQLCTDDFMGH
                     LAHNANLSVKAILAIAAYGELCRMRGDQASATKYKNLAKEYARHWIKVAADGDHYRIA
                     FDKPNTWSQKYNLVWDELLGLDIFPAEVARKEVAFYKTRLERFGVPLDSRTRLTKTDW
                     TLWIATMAKVPADFEAMVSPIHDYLNQTSARLPFVDSYMTNNAQSDGMRARPVIGGVF
                     IKMLADVATWKKWAARDRARVGNWAPAPAPPRVTEVVPTSEHKPVVWRYSFTKPGADW
                     ARPDFDDSKWKQRPGGFGKSGTPG"
BASE COUNT          756 a         1115 c         1128 g          755 t
ORIGIN      
        1 aaggtcggca aatggacagc cgccgagctc gagcacgccg ccgaacattg gcgacgacaa
       61 ttccggggtg aggcacccgt caagaatgac aacgacatca ccgaggccga catcgccgcg
      121 cacaacctta tcctctgggg cgacccgggc agcaattcgc tggtggcgaa agtgatcact
      181 aagctcccat taacctggga caaggagaac gtgcgcgtgg ggagtgcaac ctatggcgca
      241 ggtcatcacg tgccggtgct gatctatccc aatcctctga atcacaagag gtatgtcgtg
      301 ctcaacagcg gtttcacatt tcgagaatac gattacctga gcaatgcgcg gcaggttccc
      361 aagctaccgg acttcgctgt ggtcgacatc gacgtaccag tatcggctcg agcacccggt
      421 ggcgtcgcaa cggccgggtt cttcaccgag gagtggggtt tgccggcgac ggactgaccg
      481 cctgagcgag ctacggcgcg aaccgggaat cgtcgtcccg ttcatccctc gcgcaggctt
      541 tcgaggcgcc gattgccgtc aagtggcctt ccggttattt tctatttcat gaaacagctt
      601 ctacgtgaac tttccaatgc gctgttcatt atcttgggca tcttgtcggc gggaatggga
      661 attcatgggt tcttgatttc cagccacttc attgacggcg gggtcaccgg catctcaatg
      721 ctgctggcaa acgtgctcgg aataccgctg ccgatcttga ttctggcgat caacctgccg
      781 tttatcgggc ttggctatcg tcagatcggt cgcgcgtttg cgatccgcag cgcgctggcc
      841 atctcggggc tttctgtgtg cttggccacg atccattatc ccgacgtcac tcccgataag
      901 ctgttgactg ctgtattcgg tggttttttc ataggggcgg ggatgggtct ggcgatgcga
      961 ggcggtgcgg tactggacgg gaccgacatc gcggcacttt tgatcagcaa gaggagccat
     1021 ctctttcgcg tgggagacgt cattctcggg ctgaatgtcc tcatcttcct gacggcggag
     1081 tttttcctgg gtgtcgagcc ggctctgtac tcgatcttga cttatctctc agcgacaaag
     1141 acgctcgatt ttctattgca cggtcttgag gagtatacgg cgatcaccat cgtttccgag
     1201 cggagcgccg cgatacgcga agcgctcaca gtgggtttgg gacgaggcgt tactgtctat
     1261 aagggacgcg gcggaatgag cggcgcggac caggaaatct tgctgtgcgt agtaacgcgg
     1321 ctggagatcg gcaaggtgtt gaacatcgcc cacgatctgg acgactcgtc gttcatcacc
     1381 atccatcctt tggccgaggt caaggggggc gttctcaaga agactgcgca ccattaagac
     1441 aatcctatgc aaggagtaat ggcatgaaga tgttgaacaa acaagccatc gtcggtgtcg
     1501 ccgccgtgtt actgaccacc gcctcggtcc atgcagagcc cccaggtgaa tatcccacct
     1561 ttcggcctcc ggccgtgcca ttggtcgcgc ataatccata cttgagcata tggtccatgg
     1621 ccgaccggtt gacggatgac acgacacgcc actggacccg ccgcgagcat ccgctggtca
     1681 gcttgatccg gatcgacggc aaagcctatc gtctcatggg gacccagccc aaggacgtgc
     1741 cggcgatgaa acaagtcggc gtccgggtct tgccgacccg tagcatctat gactatgacg
     1801 atggccatct gcacgtcact ctcacattcc tgacgccggc attgccagaa gatctcgacg
     1861 tgctggcgcg gccgctcact tacctcacgt gggaggtgcg ctcggtcgac ggcattactc
     1921 atgtagtgtc gatctatgac agcacgagcg ggcacttggc ggtgaacgcg cccagccaaa
     1981 aggtcgaatg gtcgcgtccg gcgtgcggcg gtctgacggc attgcgcgtg gggacagtcg
     2041 accagacttt gctccagccg gctggcgacg acacgcgcat cgactgggga tatgcgtatg
     2101 tcgcggcccc agcctcgcag tcgcaaggag caatcggcgc gagcgcagca ctgctcgatc
     2161 atttcgtccg cttgggcgcg ctccccaagg aggacgatac ccgcaagcct cgcgcggccg
     2221 acgacgatcc gccgaccctc gcgattgcct tcgacctggg ctcggttggc tcgactgccg
     2281 tgccgcggca tatgatgatt gcttatgacg agatatttgc catcaagttt ctcggccaga
     2341 agctgcgacc ctactggcgg cgcgatggag cgaccgcaga tgtgctgctt acgtccgcgg
     2401 aacgagatta tgcggatctc gtgcgcaggt gtgcggcatt cgacgcggag atgatggcag
     2461 atctcgccag tgcgggaggt gcgagatatg cgcagatcgc cgcgctggcg tatcgccaga
     2521 cgctcgccgg ttgcggcctg gcggccgacg ccaataagca accgctgttc tttcccaagg
     2581 aaaatagtag caacggctgc gtggctaccg tcgacgtgat ctatcctgcc gcacctcagt
     2641 ttttgctcat gggcccggcg tatgcgaagg ccctggtcgc tccccctctc gtctatagca
     2701 cctcgccgcg ctggaagttt acgtttgccc ctcacgacgt gggcacctat ccgcaggcca
     2761 acggacaggt ctacggcggc ggcgagagct caaccaatga agccgacatg atgcccgtcg
     2821 aggagagcgc caacatgatc ttgctgtgcg ccgccatcgc caagatggag gggacagccg
     2881 aattctcgtc gacatggtgg ccgcagctca ctcgatggga ggcctacctg gaaaagtacg
     2941 gtgaggaccc cgaaaaccag ctctgcaccg atgacttcat gggccatctg gcccacaacg
     3001 ccaatctgtc cgtcaaggct atcctggcca tcgcggccta cggtgaactc tgtcgcatgc
     3061 gtggcgatca agcatccgcc acgaagtaca agaatctcgc aaaggaatat gcccgacact
     3121 ggataaaggt cgccgccgac ggcgatcact atcgaatcgc cttcgacaag ccgaacacat
     3181 ggagccagaa gtacaatctt gtgtgggacg agctcttggg cctcgatatt tttccagccg
     3241 aagtggcaag aaaggaagtc gccttttaca agacccggtt ggagcgattt ggggtccctc
     3301 tcgactcgcg cacgcgtctc accaagacgg actggactct ctggatcgcg acgatggcga
     3361 aagtcccggc cgactttgaa gcaatggtct caccgatcca cgactatctg aaccagacga
     3421 gcgcgcggct cccgttcgta gactcgtaca tgaccaacaa cgctcagagc gacggcatgc
     3481 gtgccaggcc cgtgatcggc ggcgtgttca tcaagatgct tgcggatgtt gcgacctgga
     3541 agaagtgggc tgcacgggac cgagcgcgcg tcggcaactg ggcgccggcc cccgcaccgc
     3601 ctcgagtcac cgaagtcgtg ccgacgtcgg agcacaagcc cgtcgtatgg cgctactcat
     3661 ttaccaaacc cggcgctgac tgggccaggc ccgactttga cgacagcaag tggaaacaaa
     3721 gaccaggagg gtttggcaag tccggcacgc cagg
//
LOCUS       DATAQL010000115         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4591468, whole genome shotgun sequence.
ACCESSION   DATAQL010000115 DATAQL010000000
VERSION     DATAQL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4591468"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..>2187
                     /locus_tag="VHS97_01945"
     CDS             417..>2187
                     /locus_tag="VHS97_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX3446982.1"
                     /translation="MRNSIVIYLNGKRLSAAGDAIFTSLAEFLRQRGLVGTKIGCGEG
                     DCGACTVLVGTLEDGSIRYRTIASCIQSLYQLDGTHIVTIEGVAQAGDLSPIQQAMVD
                     HHGSQCGFCTPGFVTALSGLFERADRIDEDVLREGLHGNLCRCTGYEPILEAALSVDP
                     ANVQRLASLYPSRSMYEELAECSCDSILIETGRRVFFRPTTLDEAVAFRAQHPGAVIT
                     AGGTELGVQRNKQGREPEALLSLGAITELSKISHNDGVLSVGANVTWARLEAFTHGSL
                     PEIHTLTRRFGSPQIRNAATLVGNIAHGSPVADSLCFLMISLAELELTSSHSVRRVAI
                     SDFYTGYKKTVVAPDEIITRVLIPQSAQSNGEIVKLYKISKRKEIDVSTFRAGIRIKR
                     DGQQIESAAIAYGGVGPTVRRLPATEDFLVGRPWSETTFREAGARARAEVEPISDVRG
                     SRDFRLKLAENILVKFYHDTIDAQPRDNSKAASAVTHHARDMAIDIQNVHESARAHVT
                     GKAVYVDDLPPFRNELLVEFVGSPLAHGRIVTLDVNKAAQLEGIAAVLTAADVPGDNN
                     FGPIFHDEEVLAAHECHHIGQPIV"
BASE COUNT          435 a          675 c          653 g          424 t
ORIGIN      
        1 caacccaata tcctattttc ggagccggcg acgatttgac aatgggttta ccgcagatta
       61 caccgcgcaa tcgagcaggt cgttcactag cgcgatcgcg caccgcgtgc taacgtgcga
      121 acagacgttc acactcgccc ccttttgtgg gagagggcca gcatggggat acggcgcggt
      181 gctccgcgcg gacgcaatgc gtgtcagtgc cttacgtccg caaaaacctg acggcaacct
      241 tcacactgga ccagtccgcc atcgtttaac ggagggcttc ttacctcaca ccgcgacgta
      301 atgtgacgtg aatcctggga cgagattgat cgacctctgc gcgggccaat tgttataacc
      361 gatgcatggc aacgttcctc gacagcgcac tctaagttcg aaattcaaaa gatgctatgc
      421 ggaattcgat tgttatatac ctgaacggaa aacggctgtc ggccgccggc gacgccatct
      481 tcacgtcact ggccgaattc ctccggcagc gcggcctggt cggcacgaag ataggctgtg
      541 gcgaaggaga ctgtggcgcc tgcacggtcc tggttggtac gctcgaagac gggtccattc
      601 gctatcggac gattgcttcg tgtattcaat cgctttacca gctcgacggg acccacatcg
      661 tcacgatcga gggtgtcgct caagcggggg acttgagccc gattcagcag gcgatggtcg
      721 accaccacgg gtcgcagtgt ggattctgta cccccgggtt tgtcacagcg ctcagcgggc
      781 tattcgaacg cgcagatcga attgacgaag acgtgctccg cgagggcctg catggcaacc
      841 tttgtcgctg caccggctat gagccgattc tggaagccgc tttgtccgtc gatccagcaa
      901 acgtacagcg tctggcgagc ctttacccct ctcgctccat gtatgaagag ctcgctgagt
      961 gctcttgtga ttcgatcctg atcgagacgg ggcggagagt cttcttccgg ccgacgacac
     1021 tggacgaggc cgtcgcattt cgggcgcaac atcccggcgc agtgatcact gccggcggca
     1081 cagaactggg tgtccagcgc aacaaacagg gtcgcgagcc cgaggcgctc ctgagcttgg
     1141 gcgccatcac cgaactgtcg aaaatcagcc acaacgacgg cgtgctctcg gtcggcgcca
     1201 acgtgacctg ggcgcggctc gaggcgttta cgcatggcag cttgcccgaa atccatacgc
     1261 tcacccggcg gttcgggtcg ccgcagatcc gcaatgctgc cacgttggtg ggcaatatcg
     1321 cccacggttc tccggtcgct gactcgcttt gctttctgat gatctccctt gccgagctgg
     1381 aactgacgag ctcgcacagt gtgcggcgcg tggcaatctc tgacttttat accggctaca
     1441 aaaagaccgt ggtcgcaccc gatgagatca ttacccgggt gctcatcccg caaagcgctc
     1501 aaagcaatgg cgagatcgtg aagctctaca agatttccaa gcgcaaggag atcgacgtct
     1561 ccacgttccg cgccggcatc cggattaaac gagacggcca gcagatcgag tcagccgcga
     1621 tcgcctatgg cggcgtcggg cctaccgtgc ggcgattgcc cgcgaccgag gactttctgg
     1681 tcggccggcc ctggtcggag acgacttttc gcgaggcagg cgcgcgggct cgcgccgagg
     1741 tcgagccgat ctccgacgtc cgcggctcgc gcgacttccg cctgaagctc gcggaaaaca
     1801 ttctcgtcaa gttctatcac gacacgatcg acgcgcaacc tcgagataac agcaaggctg
     1861 cgtcggccgt aacccaccac gcccgcgaca tggccatcga tattcaaaac gtacacgaat
     1921 cggcgcgcgc gcacgtcacc ggcaaggcgg tgtacgtgga cgacttgccg ccgtttcgca
     1981 acgagctttt ggtcgagttc gtcggcagcc cgctggcgca tggacggatc gtcacgctcg
     2041 acgtgaacaa ggccgcacag ctcgagggga tcgcggccgt attgactgcg gccgacgtgc
     2101 ctggtgacaa caatttcggc ccgatcttcc acgacgagga ggtgctggcc gcgcacgagt
     2161 gccatcatat tggccagccg atcgtgg
//
LOCUS       DATAQL010000116         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1844362, whole genome shotgun sequence.
ACCESSION   DATAQL010000116 DATAQL010000000
VERSION     DATAQL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844362"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VHS97_01950"
     CDS             complement(<1..279)
                     /locus_tag="VHS97_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3446983.1"
                     /translation="MSSRSYRLGVQIVCGTVFASAIVCSIWVLLNPPKSPPAAQDLGA
                     AALPLGEFQLQERSGRTVRETDLNERVWIASFIFTRCPLSCPRISGVMK"
     gene            complement(312..782)
                     /locus_tag="VHS97_01955"
     CDS             complement(312..782)
                     /locus_tag="VHS97_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX3446984.1"
                     /translation="MVETTKMSASEVAAEHATESHAPYLKVWAGLAVLTLVEYYYASI
                     FKSQFLILLLGLLFLAVVKAGMVGWYFMHLKFEGKWVYIMIVPAMILATIIVLALTPD
                     MVLKPETEENQVEEYARIAPAAERPGTILAARAESPDPRASARGSRGGSVNQTL"
     gene            complement(846..1796)
                     /locus_tag="VHS97_01960"
     CDS             complement(846..1796)
                     /locus_tag="VHS97_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX3446985.1"
                     /translation="MAVATHSSTKTEPAAHGSAESHSHVRMAPVSPGKVAMWLFLATE
                     VMFFTGLIGSYIVLRAGSQHTAYSNLYPPTTPLKRLENTHGVLLKSPGGDATRVEHIL
                     HTSGGLTEEQAEEVVHEAPHGLVSRLTKEKAASLIAELRSAGAEVEDEPLKTHNWPLP
                     YDELTNPLAINLTAFNTFVLICSSVTMVLALSAFQEDKKRKGSGYLLATVLIGSVFLS
                     IQVYEYYQLMVGHHYPPGISATGHFRPSVSLFASCFFTMTGFHGAHVTGGVILLFVIW
                     LGSLMGKFSKDHYAPVELAGLYWHFVDLVWIILFTIVYLL"
     gene            complement(1858..2790)
                     /gene="cyoE"
                     /locus_tag="VHS97_01965"
     CDS             complement(1858..2790)
                     /gene="cyoE"
                     /locus_tag="VHS97_01965"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX3446986.1"
                     /translation="MASIDQPFLAPAASNDVLAAISGRFAAYVSLTKPRLVLLVLVTV
                     AVGFLLGIRGNLSRNTLVSLTATLAGTALVAGGAGALNQWLERDRDARMRRTSNRALP
                     SGRLAPSEAAAFGGLLVCLGTTILLLGANRLAAEVALATFVLYVCVYTPLKTWTSLNT
                     VIGAVPGALPPVIGWVAATGRLGVEAWALFLIVFLWQFPHFLAIAWIYREDYQRAGFR
                     MLTAADPKGAMTGAQAVSYALALIPAGLLPATVGLAGPIYFAGALLLGLLYLAEAARF
                     WFEASDRRARRLLRASFLYLPAILILLLLNPVPY"
BASE COUNT          637 a          873 c          910 g          489 t
ORIGIN      
        1 tttcattacg cccgaaatcc gaggacagga caggggacat cgggtgaata tgaacgaggc
       61 gatccagacg cgctcgttca gatcggtttc ccggactgtg cggccggatc gttcctgcaa
      121 ctgaaattcg ccgagcggca gcgcggccgc tcccagatcc tgagccgcgg gcggggactt
      181 gggaggattc aatagcaccc agatcgagca cacgattgcc gaagcaaaga ccgtcccgca
      241 aacaatctgc acaccgagtc gataggacct gctgctcact ttgattagac caccgtgggg
      301 gaaatggcgg gctaaagcgt ctggttcact gaccctccgc gggaaccgcg ggcactcgcg
      361 cgcggatcgg gcgattcggc ccgggcagcc aggatcgtcc ctggccgttc agcggccggc
      421 gcgatgcgcg cgtactcttc aacctggttt tcctcggtct cgggcttcaa gaccatgtcg
      481 ggcgtgaggg ccaacacgat gatcgtggcc aggatcatcg ccggaacgat catgatgtag
      541 acccacttgc cctcgaactt gaggtgcatg aagtaccagc cgaccattcc cgccttgacc
      601 acggccagga aaagtagccc caacaataga atcaagaatt gactcttgaa tatcgacgcg
      661 tagtagtact ccaccaaggt caaaactgcc aatcccgccc agaccttcag gtagggcgcg
      721 tgtgattcgg tcgcgtgttc ggcggcaacc tccgacgccg acatcttggt ggtttcaacc
      781 atcgtaagtc tcccgagcag cgaccgcggt tcgattcgtc aatccgaccg gccgggttgc
      841 gaagttcaaa gcaagtagac gatcgtaaac agaatgatcc acaccaggtc gacgaagtgc
      901 cagtacaggc cagcaagctc gaccggggcg tagtggtcct tcgaaaactt tcccatgagc
      961 gacccgagcc agatcacgaa gagcagaatc actccgccgg tcacgtgggc cccgtgaaac
     1021 ccggtcatgg taaagaagca tgaggcgaac aggctcacgc tcggccggaa gtgcccggtc
     1081 gcactgatgc cgggaggata atggtggcca accatcaatt gataatattc gtatacctga
     1141 atactcagaa agacgctgcc gatcaacacg gtcgccagaa gatagcccga ccctttgcgt
     1201 ttcttgtctt cctgaaacgc ggagagtgcc aggaccattg tcaccgacga gcagatcaag
     1261 acgaacgtgt tgaacgccgt caggttgatg gcgagagggt ttgtcagctc gtcatagggg
     1321 agtggccagt tgtgggtttt gagaggttca tcctcgacct cggctccagc ggacctgagc
     1381 tcggcgatca ggcttgcggc tttttccttg gtgagtctgc tgaccaagcc atgaggtgcc
     1441 tcatgaacga cttcttcggc ttgttcttcg gtgaggccgc cgctggtgtg caggatgtgc
     1501 tcgaccctgg tagcgtctcc cccgggcgat ttcaacaaga cgccgtgagt gttctccagc
     1561 ctcttgagcg gcgtcgtcgg tggataaaga ttgctgtagg ccgtgtgctg gctgccggcg
     1621 cgaagcacga tgtaagaacc gatgagcccg gtgaaaaaca tcacttccgt ggccaagaag
     1681 agccacatgg ccaccttgcc ggggctgacg ggggccatac gaacgtggga atgactctcg
     1741 gccgagccat gcgcggcggg ctcggtcttg gtcgagctat gggtggcgac tgccatcgtg
     1801 gtaacggccc tcgaagcatg acaaaaaggg agacgagccg ccggcggggg cgactcgtca
     1861 atacggcacg ggattcaata agagcagaat caggatcgcg ggcagataca aaaacgacgc
     1921 gcggagcagg cggcgtgcgc ggcggtcgct ggcctcgaac cagaaccgag ccgcctcggc
     1981 cagatacaag agccccagca agagggcgcc ggcaaaataa ataggacccg ccaggccgac
     2041 cgtcgccggt aacagccccg cggggataag cgccagcgcg taggagacgg cctgtgctcc
     2101 ggtcatcgcg cccttgggat ctgccgccgt gagcatccga aagccggcac gctggtaatc
     2161 ctcgcgatag atccaggcga tcgccagaaa gtggggaaac tgccagagga acacgatcag
     2221 gaacaaggcc caagcttcca cgcccagccg accggtcgca gccacccagc cgatgaccgg
     2281 aggcaacgcg ccgggcaccg ccccgatcac tgtattgagt gacgtccacg ttttgagcgg
     2341 cgtgtacaca cagacgtaga gcacgaacgt cgccagcgcg acctcggccg ccagccgatt
     2401 ggcccccagc agtaagattg tcgttcccaa acagacgagc agaccaccga acgcggccgc
     2461 ctccgagggt gccagacgcc cgctgggaag tgcgcggttg gacgttcgcc gcattcgggc
     2521 gtcgcggtcc cgctccagcc actgattcaa tgcgcccgcg ccccccgcaa ccagggccgt
     2581 cccggcgagg gttgcggtca atgacacgag cgtgttccgg gataaattgc cacggatacc
     2641 caggaggaaa ccgacggcca ctgtgaccag aaccaggagg acgagccgag gcttcgtgag
     2701 tgatacgtag gcggcgaaac ggccggagat ggcggccagg acgtcattcg aggccgcggg
     2761 ggccaggaac ggttgatcga tcgacgccat ggatttcaag caaccgcctc ccagtccatc
     2821 ttggccggct cgagcggccg gtccgaattc gtgccgaact caggcgacgg gctcgtcgct
     2881 ggcgcagcca attgacgaaa ggtccgtag
//
LOCUS       DATAQL010000117         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3370460, whole genome shotgun sequence.
ACCESSION   DATAQL010000117 DATAQL010000000
VERSION     DATAQL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3370460"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1245
                     /locus_tag="VHS97_01970"
     CDS             229..1245
                     /locus_tag="VHS97_01970"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3446987.1"
                     /translation="MTGRQKRYWQILATFLLGGAVAGVAYNIVRGHLAPINFATGAIF
                     GLMIASGIGAFEALVSQGPARLWMRSLPLVGAVAVRSLVYAAVIFPIQYYDLGVKAMG
                     LQSIGTLKEFWSATAYSIGVSVLFSLLLEISYLIGPRRFIRLIAGLYHAPREEERFVL
                     FVDVAGSTGTAERLGDRRSHTMLDRVFSIATGPVLNFGGEIDQYVGDEMIVTWPAASG
                     VGDGRALRCFLAMREELAKAAGSFEREFGHAPQVRGSLHFGAVVIGEIGDTRRDIVFH
                     GDVMNTAARLEEASRHVSGGFVVSRSARERLGPVERVGLESLGKFSPRGKLEPLEIFG
                     LLPG"
BASE COUNT          308 a          447 c          492 g          334 t
ORIGIN      
        1 acttatggga gtccagtacc ccgacaagga atcgcgtttc tgacggaagc gtcgcgctga
       61 aggtgtgacc ccgaaggcat aagtcggcgt caagcgtttg aacttggatc gattcgcttc
      121 gctcctcacg atgacccaag cgcgtcgtgc aatcgttgca gacagtccca tttgcgccac
      181 gaagaacgta tctatccgcg cggagcgacc gcgaagcgga gcaaatcgat gaccggcaga
      241 cagaaacgct actggcaaat cctggctacg ttcctcctgg gcggagccgt cgcgggcgtt
      301 gcctacaata ttgtgcgagg ccatctcgct ccgatcaact ttgcgacagg cgcaatcttt
      361 ggcctcatga ttgcctcggg catcggcgcc tttgaagccc tcgtctcgca gggacctgca
      421 cgcttgtgga tgcgctcact cccgctcgtg ggggccgtag ccgttcgcag ccttgtctac
      481 gcagcagtca tttttccgat ccagtattat gatcttggcg tgaaggccat ggggttgcaa
      541 tccataggca ccctgaagga attctggtcc gcgacggcat attcgattgg cgtttcggtc
      601 ctcttcagtc ttcttctcga aatctcatac ctcattggac cgcgcaggtt cattcgcctc
      661 attgccggcc tctaccacgc gccccgggag gaggagcgtt ttgtgctttt cgtcgacgtg
      721 gccggatcga cggggacggc cgaacggctg ggcgacaggc gaagccacac gatgctcgac
      781 cgcgtgttca gtatcgccac gggaccggtg ctcaatttcg gcggcgaaat cgaccaatac
      841 gtgggtgacg agatgatcgt gacctggccg gccgccagcg gcgtcggcga tggtcgcgct
      901 ctgcgctgtt tcctggcgat gcgggaggaa ctggccaagg ccgccggcag cttcgagcgc
      961 gagttcggac acgcgcccca ggtgcgcggc agcctgcact ttggcgccgt tgtgatcggc
     1021 gagatcggcg acaccaggcg cgacatcgtc ttccatgggg acgtgatgaa cacggcggcg
     1081 agattggaag aagccagccg gcacgtttcc ggcggatttg tcgtgtcacg gtccgcccgc
     1141 gagagactgg gtcccgtcga aagggtgggc ctcgaaagtc tgggaaagtt cagcccgcgc
     1201 gggaaacttg agccgcttga aatattcggg ttgctacctg ggtgacaggc tttatcggtt
     1261 tgccatggcc acggcgttgg gaatgactgc tatttgcgga tattgttgaa aaactcacaa
     1321 attgagcaac gacgaaaaac tcgttccggc gcgcataacg tcatctcagc tggtagtcgt
     1381 cacggcaagc cacatacgag ggtcacaggc ggcaaagcca gtcagtccgc cgaaccccta
     1441 aggaattttc catcgaggct gccactggtc ttctgaactg tgatcgagcc ggaaattcga
     1501 gtttttcaac atcgggtaat gggcgggagc tcttccacgg agggtcgtta ggatcggccg
     1561 tggtttggaa gaggagaccg t
//
LOCUS       DATAQL010000118         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5050968, whole genome shotgun sequence.
ACCESSION   DATAQL010000118 DATAQL010000000
VERSION     DATAQL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5050968"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..2437)
                     /locus_tag="VHS97_01975"
     CDS             complement(248..2437)
                     /locus_tag="VHS97_01975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3446988.1"
                     /translation="MKRLLVLSMLSLATAGCAQSKGALLRGASYPPSPVALTPVPSIY
                     DTVNSGVGGEKVAQTAIKNPDDPHWSGRAEVSVAARPVPPGTPNSPSGPGGAVPAAGA
                     APAGPALAAVQPQPAPAPPVGAAPATAPPATAPVAADLPVPSALPQSGGQGPDSLAAS
                     PSNAGPAGPAAEIGPAAAPESVSLSAAPAANDAGTPPPDSTSDAAKLTAASPVEAAVS
                     PSASAAPATSPAAGSPAPARGRDPLLGPEPDLLPAMPDLPSVTAKAKQPPPQVSQAPP
                     EAAPASVIQAPPPDGPPPLEVTPGLPPAASPQPNSAEPASPGPSAAVDLPIDADKSTA
                     VSRPATAGLAAAGLSLEAAAPPGQLSQPPRPVPGAASLSGDRVDRNVVLASSEEPKKP
                     RKDHKSVYKEPGGPVARVGDEIITYHDLIAAFKENLKKYPQLRGDGFNSAEQMQINRE
                     KDMLVRQTLAGLIDRTLLAQEAKRHIKEKKSIDRLYEEADKIWRTEEVLPLQRQYNVD
                     SESKLKEALAEEGRSLDTMRQSFRQYFLAETYLHEKLKDRIKVELPDLLRYYNEHLRD
                     HQFDRPAQITWRELVVEVEKYKSREQARKKADALLENLRRGANFQQLARSESDGPTSS
                     RNQGGLMKTTPGSYAVKSINDALDTLPINQVSGVIEGPDSFHVLLVENRRPAGPASFE
                     EIQDQIRPALENQKYRDERVAFVAKLQKNSLISIYNLSMDDSKRNSD"
     gene            complement(3049..4131)
                     /locus_tag="VHS97_01980"
     CDS             complement(3049..4131)
                     /locus_tag="VHS97_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3446989.1"
                     /translation="MATSGSRASWRSLESLFNTGALGGLTDGELLECFQSSRDTIGPE
                     AFRILVERHGPMVLGLCRSVVRDPHDADDAFQATFLVLVRKADSITRRDTIGPWLYGV
                     AGRVARRARNRSIQRKRREVPTNAEIPSTNRPFAESPSVDDIVHDEIGRLPESFRGPL
                     VLCCLEGLSYDVAARQLGLTEPTLRGRLFRARKKLASRLRERGITAGLFASAVEPVRL
                     TLPPLPSSLVETTVQFAIRWSSVTGLLSGAALIPESIGLLAQGVIKAMFLQSIKLSGI
                     VAIVAAGALGTMVVAQQANNAANTSGPPTNNAAASTPEEQAKEAKGLRRVVDRMELLT
                     IDKQTQLIKQKLDQVIDAEFPAGTTL"
     gene            4149..>4368
                     /locus_tag="VHS97_01985"
     CDS             4149..>4368
                     /locus_tag="VHS97_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446990.1"
                     /translation="MNEGRRVAFLPIILCSLSRDPHNFLISAIANRHRPHSRSSIVES
                     WRLIQLGDPAPLPVVTPHDPEHTGQVMKL"
BASE COUNT          809 a         1297 c         1381 g          881 t
ORIGIN      
        1 gatggttcga catcctcgtc tcctagtcag ctacagagcc tcgactcggt ccggagcaac
       61 agactcgtga gctgtacggc ccgcccccgc ggaacacacg tccatgagta tacacacgag
      121 agaattgggc tcgcaagatg cacggagggc ttggcggggc agtgtgcggt gatggaacaa
      181 gataaccgag atttaacagt acaattttat tcgtatgttt atagaatttt atattgtcat
      241 acaaatttta atcggagttt ctctttgagt cgtccatgct gagattatag atcgaaatga
      301 gcgagttctt ttgcagcttg gcaacgaatg cgacgcgttc gtcgcgatat ttctggttct
      361 cgagcgcagg cctgatctga tcttggattt cctcgaatga ggccggaccg gcgggccggc
      421 ggttctcgac caagaggaca tgaaagctgt caggtccctc gatcacgccg ctgacctgat
      481 tgatcggcag cgtgtcgagc gcatcgttga tagacttgac ggcatagcta ccaggagtgg
      541 tcttcatcag gcctccttgg ttgcgagagc tagttgggcc gtcgctttcg gatcgggcaa
      601 gctgctgaaa gtttgccccg cgtcgaagat tctcgagtag cgcgtcggcc ttcttgcggg
      661 cttgctcgcg gctcttgtat ttctcgacct cgacaacgag ctcgcgccag gtgatctggg
      721 caggacggtc aaattggtgg tcccggaggt gctcgttgta atagcggagc aagtcgggca
      781 gctcaacctt gatccggtcc ttgagctttt catgcaggta agtttccgcc agaaagtact
      841 gccggaacga ctggcgcatg gtatcgagag aacgcccttc ttcggcgagt gcttccttca
      901 atttcgattc gctatctacg ttatattgtc gctgtagcgg caggacctct tccgttcgcc
      961 agatcttgtc ggcctcttca tagagccgat cgatactttt tttctctttg atatgacgct
     1021 tggcctcctg ggccagcagg gtgcgatcga tcaagcccgc gagagtctga cggaccaaca
     1081 tgtctttttc ccggttgatc tgcatctgtt cggcgctgtt gaagccatca cctcgaagct
     1141 gcggatactt ctttagattc tccttgaagg cagcgatgag atcgtgatag gtgatgatct
     1201 cgtcgccaac tcgcgcgacg ggcccgccgg gctctttata gacgctctta tgatccttac
     1261 ggggtttctt aggctcttcg gacgaagcca gcacgacgtt acgatccacg cggtcgccag
     1321 acaggctggc cgcgccgggc accggtcgtg gcggttgtga caactggccc ggcggcgcag
     1381 ccgcctcgag cgacagcccg gccgcggcca ggccggcggt ggccggcctg gagacggcgg
     1441 ttgacttgtc ggcgtcgatt gggagatcga cggcagccga gggcccgggg gaagcgggct
     1501 cggcggagtt tggctgcggg ctggccgcgg ggggcagacc cggcgttact tcgagtggcg
     1561 gcgggccgtc tggcggcggc gcctgaatga ccgaggccgg cgcagcttcc ggtggcgcct
     1621 gactgacctg cggcggaggc tgcttggctt ttgcggtaac cgacggcaaa tctggcatag
     1681 cgggcagcag gtcgggctcg gggcccagta aggggtcgcg gccgcgcgcg ggggcaggcg
     1741 acccagccgc aggtgacgtc gcaggcgcag cggaagctga cgggctgacg gccgcctcaa
     1801 ccggcgatgc agccgtgagc ttggcggcgt cgctcgttga atcgggcggt ggagttccgg
     1861 catcgttcgc tgctggcgcg gcggacagcg ataccgactc gggagcggcc gccgggccaa
     1921 tctccgcggc cgggcccgcc ggcccggcgt tggaggggga cgccgcgagt gagtcggggc
     1981 cctgtccgcc agactgcggc agggccgaag gtacgggtag gtcggccgcc accggcgcgg
     2041 tggccggcgg ggcagtcgcc ggcgctgccc cgacgggcgg tgcaggcgcg ggctgcggtt
     2101 gcacggcggc caatgccggg ccggcaggag ctgcgcctgc cgccggcacg gcgccgcctg
     2161 gtccactagg agaattcggc gtaccgggcg gtactggccg agccgccacc gaaacttcgg
     2221 cacggcccga ccagtgcgga tcatccggat tcttgatggc cgtctgagcg accttctcgc
     2281 cgccgacgcc cgaattgacc gtgtcgtaga tcgagggcac cggagtcagc gcaaccgggc
     2341 tgggcggata cgatgcgccc cgaagcaatg cacctttcga ttgagcacag ccggccgtcg
     2401 ccaggctcaa catgctcaac accaacagtc gtttcatgat ttggttggcc tccatccttg
     2461 gagtttgccg accgcgtctg gagaagaggg cgcaaagaaa tgtttcgcag ccgacggcct
     2521 tcccggcctt cgtgacactt ttgagtaatc ggtctcggac gaacgtgacg gtcgtccgac
     2581 gtgattcgag acgacgccaa ggacgtgaaa atcacgcccg accaaatgac cgaacagacg
     2641 gcgacagacc gccgcgcgag gaatcttacc cggacgacct gggaaagcaa ggcgaaacgg
     2701 gaagccgcgg gatcgcaatc cttgctgcag ccatcatttc gccggccgac cgagcttgtc
     2761 caacgactcc agcactcgat caagcttgcg ttccatctcc tcaacgcgca tctccgtcac
     2821 gctcgtccgc gaatcgatca ttaagaagcc atccttaacg aaataggaaa gttgcagcgg
     2881 gtggagagcc caccaaagga gtcctcgcac tgtgcactgc tttctgttca ccacaatcgg
     2941 cactgccatg ccctggttgg cttcctgtaa gcctattggg ttcacgtaaa ttgggattcc
     3001 tgggtagttt ttgtcggtcg ttgtttgctt gatgtgtttg agaagatgtt aaagagttgt
     3061 tccggcaggg aattcggcgt cgatgacctg gtcgagcttc tgtttgatga gttgggtctg
     3121 cttgtcaatc gtcagcagct ccattctgtc gaccacacgg cgtaggccct tggcctcttt
     3181 ggcttgctct tccggagtcg acgcggccgc gttgttggtg ggcggcccac tagtattagc
     3241 cgcgttattc gcttgctggg ccaccaccat cgtccccagc gcccctgcgg ccacaatggc
     3301 gacgataccc gagagcttga tcgattgcag aaacatggcc ttgataactc cttgggctaa
     3361 caaaccgatc gattcgggga tgagggctgc gcccgacagc agaccggtca ccgacgacca
     3421 ccggattgca aactgaaccg tggtttcaac caacgatgag ggcaggggcg gtagcgtgag
     3481 gcgaaccggc tcaactgcgg acgcgaacag gccggcggta attccgcgct cgcgcagtcg
     3541 cgatgcaagc tttttgcgag cccgaaagag ccggccgcgg agggttggct cggtcaggcc
     3601 cagttgacgt gccgccacgt cgtagctcaa cccttcgaga cagcagagga cgagcggacc
     3661 gcggaacgac tctggcagtc tcccaatctc atcatggacg atgtcgtcga cagaaggcga
     3721 ctccgcgaac gggcggttgg tgctgggaat ctcggcattt gtcgggactt cgcgccgctt
     3781 gcgctgaatc gaccggttcc gtgcccgccg tgcaacccgg ccggccactc cgtacagcca
     3841 ggggccgatg gtatcccggc gcgtgatgga atcggccttg cgcaccagga ccaagaacgt
     3901 ggcttgaaaa gcatcatcgg cgtcgtgtgg gtcccggacc acgcttcggc acagacccaa
     3961 caccattgga ccgtgccgct cgaccaggat ccgaaacgcc tcaggaccga tcgtgtcgcg
     4021 actgctctga aagcactcca gcagctcgcc atcggtcaaa ccaccgagtg cgcctgtatt
     4081 aaagagcgat tcgagtgacc gccaactggc tcgcgaacca cttgtcgcca tcgaggagtc
     4141 tcggccagat gaacgaaggt cgacgcgttg cattcctacc gataatattg tgcagcttgt
     4201 cgcgcgatcc tcacaacttc ctgatttccg ccatcgcgaa tcgccatcga ccacactctc
     4261 gcagctcgat cgtcgagagc tggcgactga tccagctggg tgacccagca cccttgcccg
     4321 tcgtcacgcc ccatgatccc gagcacaccg gccaagtcat gaaattgt
//
LOCUS       DATAQL010000119         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_622821, whole genome shotgun sequence.
ACCESSION   DATAQL010000119 DATAQL010000000
VERSION     DATAQL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622821"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2854)
                     /locus_tag="VHS97_01990"
     CDS             complement(<1..2854)
                     /locus_tag="VHS97_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3446991.1"
                     /translation="MPFFFGCRGYRHFGLALWSFLLAGAAVGSACAAMPKVADGFKIR
                     LVAAVPAVQYPSQVATAPDGSLFVGEDPMDQVGPADKAIDRILIFRDGKEPVVFADKL
                     FAIFGMVWHEGALYVMNMPHLTVFRDRDGDGKAEERKELFTDLGVRAGFPNNFNDHIV
                     SGLKIGIDGYLYISVGDKGVPKATGSDGRTAQVKGGGILRCRPDGTGLEVYSNGTRNH
                     LEPNLDDRDNLFTYDNTDDGLGWWTRVSHHIDGGYYGYPFDYHNRKDRMLPRMAEYGG
                     GSPCGGIYYGEDAWPEKYRGLFFWAEWGRRVVRAFKFEPEGASFKVAETFDFVEPGGV
                     DDFRPLDLALANDGKTMYIADWSFGAWNNKTEKLGRVYAVTYDRATRSRTRPRRKDSD
                     SIDVQIQQLAHPSFNERSRAQASLVRRGKTALPAVTAALADSATSPVAKRHLVWVIDA
                     IAGGTPEATYPLIDALKSANRDVRAQAARALGERAVPIAMEPLLKRLRDGEPAVRLQV
                     VIALGRIGQAQAIGALLPLVADPDVYLAYSVRQALRRIGDWPAIARGLDSTDVKVRSG
                     VLLAIEQVYDVAAAGELIRFAASPQRPADERVKAIAYLGEVARKAPPWDGKWWGTQPA
                     KRNPPSKTIAWDGTPRVMATIRELTTDKSAAVRKAAVDALVNTNDRDSRPLLRSRFTA
                     EQEPDVKSAIARAVGKLGDTESLDLLISALRDNRMAEPVRDAALESIEMIGSTKAVTA
                     LVDVLDQKTMGVDKLPRVIAALGRFKDAKAKKPVMEALKSPAAAVREAAIDALAAIVK
                     ANKESMRDDVIPSIRALLNDPAAEVRNRAIAGTAAIGDRQAIPALIELLDKAESRFEA
                     GLALAALPDVRALQVYLRGLTEKNADLRKASAGAIAAIRESAVVVLDQLSKRNELPQA
                     ALPELRSIFTALAPIAAWHVIGPFQFDQKPNLGDKES"
     gene            complement(3066..3923)
                     /locus_tag="VHS97_01995"
     CDS             complement(3066..3923)
                     /locus_tag="VHS97_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3446992.1"
                     /translation="MTNLAEPEALPAPARIAVAIPCYNEAAAIATVVAQFRAALPAAR
                     IVVFDNNSTDGTGEIARALGTCVILVREQGKGHAVRAAFATLTEEDVLVLTDGDGTYP
                     AECAPLLVTQVLDGHADMAVGARQPLPGAGAMSLTRGLGNQLIRAAFWVLVGPGNTDL
                     LSGYRAFSKRYRALVRLRSSGFEIETELASEAVARRLRVVEIPTPYHPRIAGTESKLR
                     AFRDGRRILGTIVLQSFRLRPHRLLFAWVAPFLVLALTVHRAFAAIGGVGLIALWGLV
                     LIDIRVRRQ"
BASE COUNT          760 a         1313 c         1277 g          728 t
ORIGIN      
        1 cggattcttt gtcgccaaga ttcggcttct ggtcgaactg aaacggtccg ataacatgcc
       61 atgcagcgat tggcgccagg gcagtgaaga tcgagcgaag ctcgggcagc gcagcctgcg
      121 gcagctcatt gcgcttcgag agctgatcga gtacaactac agccgattca cgaatggcgg
      181 cgatcgcgcc ggccgaagct ttgcgcaggt cggcgttctt ttcggtcaga ccgcgaagat
      241 agacctggag agcgcggacg tcaggcagcg ccgccagcgc caaaccggcc tcgaaccgcg
      301 attcggcctt gtccaacagc tcgatcagtg cgggaattgc ctgacggtcg ccgatggctg
      361 ccgtgccagc gatcgcacgg ttgcgcacct ccgccgccgg gtcgttcaaa agagcccgaa
      421 tgctcgggat cacatcgtcg cgcattgact ctttattcgc cttgacaata gcagccaacg
      481 catcgattgc cgcttcgcgt accgcggccg ccgggctctt caaagcctcc atgaccggct
      541 tcttcgcctt ggcatccttg aatcgaccga gtgccgcaat cacgcgcgga agtttgtcga
      601 cgcccatggt cttttggtcg agcacatcga ccagcgcggt gacggcctta gttgaaccga
      661 tcatctcgat cgactcgagg gccgcgtcgc ggaccggctc ggccatgcgg ttgtcgcgaa
      721 gggcagaaat caagagatcg agcgactccg tatcgcccag cttgccgact gcccgggcga
      781 tcgcgctttt gacgtcgggc tcttgctcgg ccgtgaatcg gctgcggagc aaagggcgcg
      841 aatcgcggtc gtttgtgtta actaatgcat ctacggccgc cttgcgtact gcggccgatt
      901 tgtccgtcgt cagctcgcgg atggtcgcca ttacgcgcgg ggtaccgtcc cacgcgattg
      961 tttttgacgg cggattgcgc tttgccggct gagtgcccca ccacttgcca tcccagggag
     1021 gggccttgcg agccacttcg cccagatacg caatagcctt gacgcgctca tcggccggtc
     1081 gttggggcga ggcggcaaac cgaatcagct cacctgcggc cgcgacgtcg tacacctgct
     1141 cgatcgccag taagacaccg gagcgcacct tgacgtcggt tgagtcgaga ccgcgcgcga
     1201 tggccggcca atcgccgatc cgccgtagcg cctgccggac cgagtaagcc agatagacgt
     1261 caggatcggc taccagcggc aagagcgctc caatcgcctg ggcctgcccg attcgcccga
     1321 gcgcgatcac cacctgcagc cgcactgcgg gctcaccgtc cctgagccgc ttcaacagtg
     1381 gttccatcgc gatcggcacg gcccgttcgc ccagggcgcg cgccgcctgg gcacgtacgt
     1441 cgcgattcgc cgatttgaga gcatcgatca acggataggt ggcctccgga gtgccgccgg
     1501 caatggcgtc gatcacccac acgagatgcc gcttggccac cgggctggtc gcgctgtccg
     1561 cgagcgcggc cgtcaccgcc ggcagcgcgg tcttgccgcg gcgcaccagg ctcgcctggg
     1621 ctcgagaccg ttcgttgaac gagggatggg cgagctgttg gatctggacg tcgatcgagt
     1681 cggagtcttt gcgccgcggc cgcgttctcg accgtgtcgc tcggtcgtaa gtaaccgcgt
     1741 agacgcgtcc tagtttctcg gtcttgttgt tccaggcacc gaacgaccaa tcggcgatgt
     1801 acatggtctt gccatcgttt gcaagcgcca ggtcaagcgg gcggaagtcg tcgactcccc
     1861 cgggttcgac gaagtcaaac gtttcggcga ccttgaagct cgctccttcg ggctcgaact
     1921 taaacgcgcg aacaacgcgc cgaccccact cggcccagaa aaacagtccc cggtactttt
     1981 ccggccaggc gtcctcaccg taatagattc cgccgcaagg tgagccccca ccgtattcgg
     2041 ccatccgggg gagcatccgg tctttgcggt tgtggtagtc aaacgggtag ccataataac
     2101 cgccgtcaat gtgatgagag acccgcgtcc accaccccag gccgtcatcg gtgttgtcgt
     2161 aggtgaaaag attatcgcgg tcgtcgagat taggttccaa atggttgcgg gtgccgttcg
     2221 aatagacctc gagcccggtc ccgtccggcc ggcagcggag gatgccgccc cccttcacct
     2281 gcgcggtccg cccgtcggag cccgtcgcct tgggcacccc tttgtcaccc acggagatat
     2341 ataggtatcc atcgatgcca atcttcagtc ccgagacgat atgatcgttg aaattgttgg
     2401 ggaagccggc ccgcacgccg aggtcggtga atagctcttt gcgctcctcg gccttgccgt
     2461 cgccgtcgcg atcgcggaag acagtcaagt gaggcatgtt catcacatag agtgctcctt
     2521 catgccatac catgccgaag atcgcgaaca acttgtcggc gaagacaacg ggttccttgc
     2581 cgtcgcgaaa gatgaggatt cggtcgatcg ccttgtcggc gggcccgacc tgatccatcg
     2641 ggtcttcgcc gacgaatagc gaaccgtcgg gcgcggtcgc gacctgtgag ggatactgca
     2701 cggccggcac cgcggccacc aggcgaatct tgaaaccgtc cgccaccttg ggcatggcgg
     2761 cgcaggccga gcccacggcg gcgcctgcga gcaagaaact ccaaagcgcc aagccgaagt
     2821 gccggtaacc ccggcaacca aaaaagaagg gcatcgtcgt ctggttcctt aacgatggaa
     2881 agggttgcgg aagggagaca agtctggtca agccgccagg caacccggcg gcagcgatct
     2941 gctgtgcgcc tcggtacgcc ggaacctgtc ctgcacagta taaattcggt gcccggccag
     3001 cgacaaccgt ggccgtcgcg cggtccgata tcaccaatcc cacgtcattc acgcccgatt
     3061 ctcacctact gacgccgcac ccggatgtcg atcaacacga gaccccagag cgcgatcaga
     3121 cctacgccgc cgatcgccgc gaatgcccgg tgaactgtga gcgcgagcac caggaatgga
     3181 gcgacccacg cgaatagcag tcgatgagga cgaagccgga agctctgaag cacgatcgtg
     3241 cccaatatcc gccggccgtc gcgaaacgcc ctcagtttgc tctcggtgcc ggcgatgcgc
     3301 gggtgatagg gagtgggaat ctcgacgacc cgtagccgtc gtgccacggc ctcgctggcc
     3361 agctcggtct cgatctcgaa ccccgacgac cgcagccgca caagggcgcg atagcgtttg
     3421 ctgaaagcac ggtaaccgga aaggaggtcg gtgttgcctg gtccgaccaa gacccagaag
     3481 gccgcgcgga tcagttggtt tcccagtccg cgggtcagtg acattgcccc ggcgccgggc
     3541 aacggctgac gcgcgccgac cgccatgtcg gcgtggccat caagaacctg agtcacgagc
     3601 agaggagcgc actcggccgg gtacgtgccg tcgccatcgg tcaagacgag cacatcttcc
     3661 tcggtaagtg tcgcgaaggc cgcgcgcacc gcgtgcccct ttccttgctc gcgaactagt
     3721 atgacacacg tgccaagcgc gcgggcgatc tcgccggtgc cgtcggtcga gttgttgtcg
     3781 aacacgacga tcctcgcagc cggcagcgcg gcccgaaact gggcgaccac ggtcgcgatc
     3841 gccgccgcct cgttatagca cgggatggcg accgcgatcc gagccggagc tggaagagcc
     3901 tcgggttctg cgagattggt cactttcccc cccttgtgaa gggggggccg gggggggtgg
     3961 tcattgagcg atcaggcaca cggattccaa cgagcttgcc aggaacgaag cactttcccc
     4021 ccctcgtgaa gggggggcca gggggggcgg tcagtgaaca atcaggcaca attattgc
//
LOCUS       DATAQL010000120         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_318047, whole genome shotgun sequence.
ACCESSION   DATAQL010000120 DATAQL010000000
VERSION     DATAQL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318047"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1637
                     /locus_tag="VHS97_02000"
     CDS             252..1637
                     /locus_tag="VHS97_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3446993.1"
                     /translation="MFDPNPFVSRRDILKAVSSGFGYVAFAGLSTIAAEHDRKRPLAS
                     RPPQFPARAKRVIFLCMHGAPSHVDTFDYKPRLAADTGSPSPIGRLAGAKLLGSPWSF
                     SQRGQSGPWISELFPEVAGHADELCIIKSMHTDLPAHPQAYLRMHTGSSQFLRPSLGA
                     WTLYGLGTENENLPGFIAIKPVARNGGPQNYGSAFLPAIYQGTRIGNEVTPVANAQLG
                     NIKNPRLGPAQQRSQLDLVQSMNRELLAREQAHPGVEGIIESYELAFRMQGSLPEAMD
                     LANESDATKKLYGIDDDETADFGRQCLLARRFVEAGVRFVELSHGDWDQHRNLRTDHA
                     RHARAVDKPIAGLLTDLGSRGLLKDTLVIWGGEFGRTPHAQNGDGRDHNNKGYAIWMA
                     GGGAKPGLSYGQTDDHGYEAVDHPVHVHDWHATVLHLLGLDHEKLTYRYAGREMRLTD
                     VKGRVVKDIIA"
     gene            1651..1971
                     /locus_tag="VHS97_02005"
     CDS             1651..1971
                     /locus_tag="VHS97_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446994.1"
                     /translation="MRHATVTRLMVILTAVITAASATTGGELPPGRRHVLSIDGEKTV
                     TFVPQANLAPLRIDYHARVEYIVNTRTGDEAFATKKTGGKNAVTAKNKRKAGGAPSPK
                     RRRP"
     gene            2076..3041
                     /locus_tag="VHS97_02010"
     CDS             2076..3041
                     /locus_tag="VHS97_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX3446995.1"
                     /translation="MDTAADDLPPQARRLGPKLRALADRGVYIGTSSWKYEGWLGSIY
                     TEGRYLTRKKFSKAKFEQDCLDEYAETFPTVCGDLTFYQFPSEQYWAKLFDATPKQFI
                     FGFKVPEDITVETWPKHARYGKRAGLPNENFLNATALEQFFTRRLMPYGQRVGPLIFE
                     FGTFNRTTFPRPADFMAKIDAFLKALPEGFRYAIEIRNDEYLTAEYLDILASYNVAHT
                     LNAWTRMPALDVQAQLPGVLTADFTVVRALLRKGRPYDKAVETFQPYDRIQEPNESAR
                     EGMRLIAQRAMHERKDAFLFVNNRLEGNAPSTIEVVVERIGTLDY"
     gene            3809..>4137
                     /locus_tag="VHS97_02015"
     CDS             3809..>4137
                     /locus_tag="VHS97_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446996.1"
                     /translation="MPNPPGIPAGGIFRQATAHSSRATAGVLSLDPVAVDLSPSSAGP
                     GVAAGFRGGQPSSLAAQAVATTLHFGAIQSSLKSSPESTPRTVPIVTAVTPGQQRAEL
                     PDIPALAP"
BASE COUNT          847 a         1346 c         1194 g          750 t
ORIGIN      
        1 actccgattc acgacgactg attgatcacg tcctcgtccg cgtccggcgg cagaggacgt
       61 gatcaatcag tcgtcgtgaa tcggagtcgc cggacatggc cgtcagccct ccccgcttgc
      121 tgtggcacgg gcttccagcc cgtgtctgaa aataccacgg gcaagatgcc cgtgccacat
      181 aaggccgccg agcccgcttg ctcaatctat ttttcgcggt tcctagcggc cgcttagcag
      241 gactgcctct catgttcgac cccaatccgt ttgtctcgcg ccgtgatatc ctcaaggccg
      301 tttcctcggg cttcggctac gtggcgtttg ccgggctgtc gacgatcgcc gccgaacatg
      361 accgcaagcg ccccctggcg tcacgaccgc cgcaatttcc ggcccgggcg aagcgggtga
      421 tcttcctctg catgcacggg gcaccgtcgc atgtcgacac cttcgactac aagccccgcc
      481 tcgccgccga cacaggcagt ccctcgccca tcggcaggct ggccggcgcc aaactactgg
      541 gctcgccctg gagcttcagc caacgcggcc agagcgggcc gtggatctcg gagctctttc
      601 ccgaggtcgc cggccatgcc gatgagctct gcatcatcaa gagcatgcac accgacctgc
      661 ccgcccaccc gcaagcgtac ctgaggatgc atacgggcag ctcgcagttt ctaaggccgt
      721 cgctgggggc atggacactt tacggcctcg gtaccgaaaa cgaaaacctc cccgggttca
      781 tcgccatcaa gcccgtggcg cgcaacggcg gcccgcagaa ctacggcagt gcctttctgc
      841 cggcgatcta ccagggaact cgcatcggca acgaggtcac gccggtcgcc aacgcccagc
      901 tcggcaacat caaaaacccg cgcttgggtc ctgctcaaca acgtagccag ctcgacctcg
      961 tccagtccat gaaccgtgag ctgctggcgc gcgaacaggc ccaccccggc gtggaaggga
     1021 tcatcgaatc gtacgagctg gcgttccgga tgcagggctc gcttcccgag gcgatggacc
     1081 tggccaatga gtcggacgcg accaagaagc tatacgggat cgacgacgac gaaaccgccg
     1141 acttcggccg ccagtgcctg ctcgcgcgcc ggtttgtcga ggcgggcgta cgattcgtcg
     1201 agctctcaca cggcgactgg gaccagcacc gcaacctgag gaccgaccat gcacggcatg
     1261 cccgggccgt cgacaagccg atcgccggac tgctcaccga cctgggcagt cgcggcctct
     1321 tgaaagacac gctggtgatc tgggggggcg agtttgggcg taccccccac gcccagaacg
     1381 gcgacggccg cgaccacaac aacaagggct acgcgatctg gatggccggc ggcggcgcga
     1441 agcccggtct ctcctacggt cagaccgacg accacggcta cgaggcagtc gatcacccgg
     1501 tacatgttca cgactggcac gccacggtcc tgcacctttt gggcctcgac cacgaaaagc
     1561 tcacctatcg ctacgccggc cgcgagatgc ggctgaccga cgtcaaggga agggtcgtca
     1621 aggacatcat cgcttgaaga gcagccgttc atgcgccatg ccaccgtaac gcgcttgatg
     1681 gtcatcttga cggcggtcat caccgcggcc agtgccacga ccggcggcga gcttcccccg
     1741 ggccgccggc acgtgctctc gatcgacggc gaaaaaactg tcaccttcgt gccccaggcg
     1801 aacctcgccc cgctaagaat cgactaccac gcccgcgtcg aatacatcgt caacacgcgg
     1861 actggcgacg aagccttcgc caccaagaag accgggggga agaacgccgt caccgccaag
     1921 aacaagcgaa aagcgggggg cgctccgtcg ccaaagcgtc gccgcccttg acctggcgat
     1981 tcacccgtcc gagatgaagt tcaaccagaa cggccagacg gtcgtcgaat cacaacttgc
     2041 aatgcctggc ctttttctgg tgggatagta gagtcatgga caccgctgcc gatgacctgc
     2101 cgccgcaagc tcgtcgattg ggccccaagt tacgggcctt ggccgatcgt ggtgtctaca
     2161 ttgggaccag ttcctggaag tacgaaggtt ggctcggctc gatctatacc gagggccgat
     2221 atctcactcg gaaaaagttc tcgaaggcta agttcgagca ggattgtctg gacgaatatg
     2281 ccgagacgtt tccgacggtc tgcggcgacc tcacgttcta ccagttcccg agcgagcagt
     2341 actgggcaaa gctcttcgat gccacaccca agcaatttat cttcggtttc aaggtcccgg
     2401 aagacatcac cgtcgagaca tggcccaagc atgcgaggta tgggaaacgg gctgggctcc
     2461 cgaatgagaa cttcttgaac gcaaccgccc ttgaacaatt tttcacgcgg cggctcatgc
     2521 cctatggtca gcgggtcggg ccgctgattt tcgagtttgg gacattcaac cgaacgacgt
     2581 ttcccaggcc agcggatttc atggcgaaga tagatgcatt cctcaaggcg ttaccggaag
     2641 gctttcgata cgccatcgag atccgcaacg acgaatacct gacagcggaa tacctggaca
     2701 tactggcctc gtataacgtc gcccacacgc ttaatgcgtg gacgaggatg ccagcgttgg
     2761 acgtgcaagc ccagcttcca ggcgtcctga cggctgactt tacggtagtc agggcgctat
     2821 tacggaaggg ccggccctac gacaaagccg tcgagacctt tcagccctat gatcggatcc
     2881 aggaaccaaa tgagagtgcc cgggaaggta tgcgactcat cgcacaacgc gcgatgcacg
     2941 agcgaaaaga cgcgtttctc ttcgtgaaca accggctcga aggcaatgca ccttcgacca
     3001 ttgaggttgt tgttgagcgg atcgggactt tggactattg aattcgatca aagtctcgaa
     3061 cgctttgcca ttgtcgtgtc gtcggatcct tcaaatgagt aagtgatccc tcccgaccgt
     3121 gtcctcgctg attgcacgca cgatgcatac gaaaaggttt cgaattcctt gcggccgcgg
     3181 tcgtcgtcga attacgacta tgctctaatg ggaaaccgca aaagaatcga ttcaacaagg
     3241 atgaatctct cgcgagagca ggtacaggat gttccacggc ttcggcgtga accatgagaa
     3301 gtcgctccac ggttctatcc aacagagaca gtccgcgcat ttcgcggtct ttcgaatcga
     3361 acttctggaa gaccgatttt tgttgtccgg cgactcggcg cgagaatttt cgggcctgag
     3421 acctacgccc gaccagaggc cggcggcgct cttcgacagc cattcacttg cgccgcgccc
     3481 ggtgtcgtcg tcaatgcggc aggtgagcgc gtcgatccgg caaacgtccg atcgtgcaaa
     3541 cacaggagtg tctatcagcg ccgtgcacca actctcttca agccggcaaa agggaatggc
     3601 gaagtcttcc aaacaccacg aatcgggcaa aaatgtttgg agcccgcagg aatcgccgaa
     3661 ctcaccccta cagtacgaac caaccaccgg cggtgccgga actacaacct cttcgacagg
     3721 ttcgggcggg gtaatcgcca ccgggccgac ggattcacaa gggcctgggg ccgcgatacc
     3781 ttgcccgcct acgccgattc cagtgccgtt gccgaatccc cctggtattc ccgccggtgg
     3841 gatctttagg caagccacgg cccattccag ccgtgcgact gccggtgtgc tctcacttga
     3901 tccagtcgct gttgatcttt cgccgtcgag cgccgggccg ggcgtggcgg cgggcttccg
     3961 cggcggccag ccctcgtcgc tcgccgcgca agccgtcgcc accacgctgc acttcggcgc
     4021 tatccaatcc tcgctcaagt catcgccgga atcgacccca cggacggtac cgatcgtgac
     4081 agcggtcacg cccggtcaac agcgggcgga gctaccagac atccccgcgc tcgcgcc
//
LOCUS       DATAQL010000121         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5203552, whole genome shotgun sequence.
ACCESSION   DATAQL010000121 DATAQL010000000
VERSION     DATAQL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5203552"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VHS97_02020"
     CDS             complement(<1..710)
                     /locus_tag="VHS97_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3446997.1"
                     /translation="MSDSAIASGVLSRRTLIAGGAAAALSAASYRRVLGANDRIGLGF
                     IGFGLIGKRHVLDFRDQPDAKVVAIAEAHGGRRDEAVALAGGSARAYADFRSLLDNRD
                     IDAVVVSTPDHWHALMTMMACAAGKDVYVEKPLSLFVREGRWMVDVAKRHNRVVQVGT
                     QQRSGPHYQQARELVRGGQIGQIVAVRMWSYRNVMPGFGSPADCDPPPGLDYDMWLGP
                     APKRPYNPNRALYHFRWF"
     gene            994..1698
                     /locus_tag="VHS97_02025"
     CDS             994..1698
                     /locus_tag="VHS97_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3446998.1"
                     /translation="MAKNHGARQQKRVAKQKAKRSAKRSLWSRLNSKDPAIRLQHVEK
                     LPVVQAFVASQLWKDGIGYLLIARQESDGGLVFAVFLVDVYCLGVKNAFWRAGTRGDL
                     DEMIRKLESTQRMSAITPECLVKIVKEAVEYAQSFGFPPHPDYRHASKLLDGIDSSSC
                     PNQFEFGRDGRPFYIQGPNESLALATAISQRVQSAGGHFIHKLSGPDPEEFGAGEDEF
                     DLLDSTDADDFPDESS"
     gene            1827..2231
                     /locus_tag="VHS97_02030"
     CDS             1827..2231
                     /locus_tag="VHS97_02030"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3446999.1"
                     /translation="MVSQNPNDTVRGRGRLADASLTPEQQASVDARRAERQLPQYQDD
                     LARDIGAYRQEYPPAGDRELNEAFAELRRERERQGLSLTEMAERTGIDRATISKLETG
                     KLNNPTIGTLRTYAKALGRRLAWTLEVVATLE"
BASE COUNT          537 a          704 c          785 g          447 t
ORIGIN      
        1 cagaaccagc ggaagtggta gagcgcccga ttcgggttat atggccgctt cggggcagga
       61 ccgagccaca tgtcgtaatc gagtccaggg ggcggatcgc agtccgcagg tgaaccgaag
      121 ccgggcatga cgttacggta cgaccacatc cggacggcga cgatttgccc gatctggccg
      181 ccgcgcacca gctcgcgcgc ttgctggtag tgcggaccgg aacgctgctg ggtgccgacc
      241 tgtacgactc ggttgtgtct cttggcgacg tcgaccatcc accgtccctc gcgcacgaac
      301 aaggaaagtg gtttctcaac gtaaacgtcc ttgcccgccg cacaagccat catggtcatg
      361 agggcgtgcc agtggtcagg cgtcgaaacg acgaccgcat cgatgtcgcg gttgtcgaga
      421 agagaccgga agtcggcgta agcgcgggcc gagccccccg cgagcgctac ggcctcgtcg
      481 cgccgcccgc catgtgcctc ggcgatggcg acgactttgg catctggctg gtcgcggaaa
      541 tcgaggacgt gccgcttgcc gatcaagcca aaaccaatga aacccaggcc aatccggtcg
      601 ttcgctccca aaacccgccg gtaagaagct gcggaaaggg ccgcggcggc tccgcccgcg
      661 atcagagttc gccggctcaa aacgcccgaa gcaatggcag agtcggacat ggtctcgaac
      721 tcctcaaaag atgagtgccc tcggatatgc ctcgcaacct taacttacag aggtgattag
      781 gtcgctgcaa gcaggtcaga taacagagcc taggcgtttt cggtggaaag ccgtaggggc
      841 agtggttcaa gtcggcgagc ctttggtatc atcgcggtct tgagattggg atgggcggcc
      901 gtaggttgtc gctgggggtg tcgcggcgga gcgcggcact caataatctg gtccggccgt
      961 ccaaggttca caaggagtgg agggcaagga gaaatggcca agaatcacgg agcaaggcag
     1021 caaaaaaggg tggcgaagca gaaagcgaaa cgctcggcga agcgttccct ttggagtcgt
     1081 ctcaactcga aagacccagc catccggctt caacacgtcg agaaattgcc ggttgtccag
     1141 gcctttgtgg cgtcacaact ctggaaggac gggattggtt acctcctgat cgcacggcaa
     1201 gagtccgacg gagggctagt cttcgccgtt tttctcgtgg atgtgtactg cctcggagtg
     1261 aagaatgcgt tctggcgcgc tggcacgcgt ggggatctcg atgagatgat ccgaaagttg
     1321 gagagcaccc agaggatgtc ggcgatcact ccagagtgtc tggtgaagat cgtcaaagaa
     1381 gcggtcgagt acgcgcagtc cttcggcttc ccgccccatc cggactatcg ccatgcctcg
     1441 aaactgctcg atgggatcga ttcctcgagt tgcccgaacc aattcgagtt cggccgcgat
     1501 ggccggcctt tctacatcca agggcccaat gagtcactcg cactggccac ggcgatcagc
     1561 caacgcgtcc aaagtgcggg aggacacttc atccacaaac tatctgggcc cgatcctgag
     1621 gagtttggcg ccggggagga tgagtttgac ttgcttgatt caaccgacgc agatgacttc
     1681 cctgacgaat cgtcctgaac cggagttgga agggaagcgg tgcggaacag cccgatattt
     1741 agacaagcca tcttcgccga tgccggccaa ggttgctcgg ttccgcgtat tgccctgttt
     1801 ctgcttacga aatcgaggaa tgaaccgtgg ttagccaaaa tcccaacgac acagtccggg
     1861 ggcgtggacg actagcggat gcctcgctga ctccggaaca gcaggcctct gtcgacgcgc
     1921 gtcgggctga acgccaactt ccgcaatatc aagacgatct cgcccgggat atcggagcct
     1981 atcgacagga atacccgccc gccggagatc gggaattgaa tgaggccttc gccgaattgc
     2041 gacgcgaacg tgagcgacag ggcttgagcc tcacagaaat ggccgagcgg acaggaatcg
     2101 acagagcgac gattagcaag ctcgagacag gcaaactaaa caatccgacg atcggtaccc
     2161 tcaggacgta cgccaaggca ctcggacgcc ggctggcatg gacgctcgaa gtggtcgcca
     2221 ctctggaata gggccaactt cgctttcggc ggaagccgaa tagggctggg gcggaactct
     2281 gacacggtcg tcgttattca cggtggagcg gcggcattta ccgctaactc gtggcgcggc
     2341 atagggggac ggttcaagcg cgagaggaat cgatttttca ttcgccagac aattgcctgc
     2401 gggcctcatc atgggtctgg gactggccgg cgcccacgcg tccgcgaatc ccgcggcaat
     2461 cggggcggaa cgt
//
LOCUS       DATAQL010000122         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1539804, whole genome shotgun sequence.
ACCESSION   DATAQL010000122 DATAQL010000000
VERSION     DATAQL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539804"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VHS97_02035"
     CDS             complement(<1..1420)
                     /locus_tag="VHS97_02035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3447000.1"
                     /translation="MAIGPGTEHCKSKVRMAAMKPSSKETRQTGTDSTSAPVERARVL
                     AGGERARTTVTPLEETAVHRSPASERLADHASLPDVESLLGRRVGQYRIERILGRGTM
                     ACVFKAKHLGLGRPCALKVMEPSLVARQPGIRAQFWAEARAAANLLHPHVVTIHNLGS
                     VQGFHFIEMEYIPGGQTLRESLIREGPLEPVRASTLARQIVLALGAAHEAGLVHRDIK
                     PANVLLTPDGRAKLADFGLVRRLDDLADGGAPLAGTPTFMAPELFRGTPASPRSDIYA
                     LGVLLYHTLSGRLPFAADTIGKLIKLHQREPIPDLRVFVPGVPDALIEILAKCLAKSP
                     ADRFGSANDLSSALQLVIHRDRDTASLVYEAAKGIDCFIQGYGDTFRVILPLRGDRLQ
                     EVVIEATEEKNNERYLSVFSVCGPADPAYYRYALTINSHLTFGSISIHDVLGSPMFVM
                     SRTFSRDTVRTEDVRDALLEIGK"
     gene            complement(1726..>2635)
                     /locus_tag="VHS97_02040"
     CDS             complement(1726..>2635)
                     /locus_tag="VHS97_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447001.1"
                     /translation="AGDILGRLEAAKELAEKHKDDKIACEALAKAWIREKEPLARTEM
                     VRLVAAVGEPCRAAILNAAKDAEPRVRVAALGGLAALKCDADAEAILRATWSNKAEAY
                     GARSAALRALVSAKVNDADELIASALKDPSDPHSLAPSALQTILDRGGQKAREAAVLY
                     SRPGQPSPLRAVAIQALSKQAKDDPQAEKLLIALVDDPVQSVRTSAMFAVTSGGFTAA
                     VPALERQLPKINGPMRQLLKAQIENLKNGTKTDKVASDSRANEPADLERQAADLELQA
                     KELRNRAEALKLKAERAKLATSKPTS"
BASE COUNT          469 a          815 c          826 g          525 t
ORIGIN      
        1 gcttgccgat ctccagcaac gcgtcgcgga cgtcttcagt gcgcaccgtg tcgcgggaga
       61 aggtgcgcga catgacgaac attgggcttc ccagcacgtc atggatcgaa atactgccga
      121 aagtcaggtg ggaattgatc gtgagggcgt acctgtaata tgcgggatcg gccgggccgc
      181 agaccgaaaa taccgagaga taccgctcgt tgttcttttc ttccgtcgct tcgatgacga
      241 cctcctgcag acggtcaccg cgcaacggga ggatcaccct gaacgtgtcg ccataaccct
      301 ggatgaaaca atcaatcccc ttggcggcct cgtaaaccag gcttgcggta tcgcggtcgc
      361 ggtgaatcac gagctggagc gcgctggaga gatcgttggc gcttccaaac cgatcggcgg
      421 gtgatttggc caggcacttt gcgagtatct cgatcaacgc atctggtacg cccggaacga
      481 acacgcgaag gtcggggatt ggctcgcgct ggtgaagctt gatcaacttg ccgatggtat
      541 ccgcggcaaa cggcaatcgg cccgacagcg tgtgatagag gagaacgccc agggcataga
      601 tgtctgagcg tggacttgcg ggcgtacccc ggaagagctc aggggccatg aaggtgggcg
      661 ttccggccag aggtgcgcca ccgtcggcca ggtcatccag ccggcgcacg agaccaaagt
      721 ccgccagctt ggcccggccg tcgggcgtga gcaggacgtt cgccggcttg atgtcgcggt
      781 gcaccaggcc ggcctcgtga gccgctccca gtgccagcac aatttgtcgc gccagggtcg
      841 acgcccgcac cggctcgagc gggccctccc gtatgagcga ctcgcgcagg gtctggccgc
      901 ccgggatata ttccatttcg atgaaatgaa atccctggac gctgcccagg ttatggatcg
      961 tcaccacgtg cgggtgcaga aggttagcag ccgctcgcgc ttcagcccag aactgggcgc
     1021 gaattcccgg ctgcctggcc accagcgatg gctccatgac tttgagcgca cacggccgac
     1081 ccaacccaag atgcttggcc ttgaacacgc aggccatcgt tccccggccc agtattcttt
     1141 caatgcgata ctggccgact cgccggccga gcaacgactc gacgtcgggg aggcttgcgt
     1201 ggtccgccaa ccgctcggaa gccggcgagc ggtggaccgc ggtctcttct aacggcgtga
     1261 cggtagtacg ggcacgctcg ccaccggcga gcactcgcgc ccgctcgacc ggggcggaag
     1321 tactatcagt tcctgtttgg cgcgtctctt tggaggaggg tttcattgcc gccatacgaa
     1381 ccttcgactt gcagtgttcc gttcccggtc caatcgccat ccgcttacag gctccttcgg
     1441 tgtttgtcgc ttcgtgccga caaggcacgc cgctgccgtc aggacacaaa atgcccccga
     1501 cgggtgcaat tcaccgcacg tcacttgaac aatacagtat tagcgaaaac cgaccaaaaa
     1561 tcgacctcaa gaccaaacga atcacctgaa gtccttgatt atcatcgtct tcagcatcgt
     1621 cggctcgtga tcgtttccgg aatcctaaca caatttcatc cgtgcttgga ttgaagatgg
     1681 cgacaagtta agcgtatgca acgatttgcg attcgttgtg cgctgtcagc tcgtcggctt
     1741 ggacgtcgca agtttggccc tctcggcctt gagcttgagg gcctcggcgc ggttgcgcag
     1801 ttcctttgcc tggagctcga ggtctgcggc ctgacgttcc aggtcggcgg gttcgttagc
     1861 gcgtgaatcg ctcgcgacct tgtcggtttt cgtgccgttc ttgaggttct cgatttgagc
     1921 cttgagaagc tgccgcatgg gcccattgat tttcggaagc tggcgttcaa gtgccggcac
     1981 cgcggccgtg aagccaccgg aggtgacggc gaacatggcg ctcgttcgga cactctggac
     2041 cgggtcgtcc accaaggcga tcaggagctt ctcggcctga ggatcgtcct ttgcctgttt
     2101 ggacagcgcc tgaattgcca ccgcgcggag aggcgagggt tggccgggac ggctgtaaag
     2161 cacggcggcc tctcgcgcct tctggccacc tcggtcgagg atcgtctgga gggccgatgg
     2221 cgccagggaa tgaggatcgg aagggtcctt gagggctgag gcgatcagct catccgcgtc
     2281 attcaccttc gcggatacca gggcccgcag tgccgcgctc cgggcaccgt aggcttcggc
     2341 tttgtttgac caggtggcgc gcagaatcgc ctcggcgtcc gcgtcacact tgagagccgc
     2401 gagcccgccc aaggccgcga cccttacgcg tggctcggcg tctttggccg cgttcagaat
     2461 ggccgcgcgg cagggctcgc cgaccgccgc gaccagccga accatctcgg tccgggcaag
     2521 cggttctttc tcgcgaatcc aagccttcgc cagggcctcg cacgcgatct tgtcatcctt
     2581 gtgcttctct gccagctctt tggccgcctc aagccggccg aggatgtcgc cggcg
//
LOCUS       DATAQL010000123         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_623544, whole genome shotgun sequence.
ACCESSION   DATAQL010000123 DATAQL010000000
VERSION     DATAQL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623544"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VHS97_02045"
     CDS             complement(<1..216)
                     /locus_tag="VHS97_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447002.1"
                     /translation="MCRKALSRGLALWFASMGVACGQTVPTAARIESVPIELSMPERY
                     QVAESLEPIRQVTLVAPADGFIRSMEIR"
     gene            456..1811
                     /locus_tag="VHS97_02050"
     CDS             456..1811
                     /locus_tag="VHS97_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447003.1"
                     /translation="MATDRITRRDFVESMGMSAGAISLLGTTGVAAASPNDKIRLGLI
                     GAGSRGNQLLDSFFKQSDIEIIAVADVDDRHAGETAERVKKEKGNLPQSARDYRFLLD
                     RKDLDAVIIATPDHWHALPAIQAVLAGKDVYVEKPVAHNVGEGQAMLHAARKTNKIMA
                     VGTQQRSSSHFQRAVEIVRSGQLGKIFWVQTWNFENISPVGMGKYPDGEAPSHVDYDR
                     WLGPAPLRSFNPNRFHLLFRWFFDYAGGMMSDWGVHLNDIVIWALNSKGPKSVCTTGG
                     IFTTDDERDTPDTMQVVYEFPGCTLTYSMRKGNGLKYNGHEYGILFCGTDGSLVLDRS
                     GFEVIPDKVVLPYGIKLVHGDRPTRKIELKPESVTKGVDGQDAHVRNFLDCMKSRALP
                     TCDIEIAHRSTNTCHLGNISYKLGRKLEWDADNETFKNDSEANALLKREPRKGYELPQ
                     V"
     gene            1853..2131
                     /locus_tag="VHS97_02055"
     CDS             1853..2131
                     /locus_tag="VHS97_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447004.1"
                     /translation="MKKTAGHFLRICGLLIEMLGVWGVFSSTGAKNSARLQLPGGNEI
                     PLAWLAVGVGFVLWFTGTILVYFTRPTRKTPSHRFSEPTDVPPVQKSS"
     gene            2557..3786
                     /locus_tag="VHS97_02060"
     CDS             2557..3786
                     /locus_tag="VHS97_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX3447005.1"
                     /translation="MVNEQDQNSQNSMHRLDPPGRLFRLEWNLFRAVRSVAAGSVSAG
                     GMPANTDGGSLERPPSDVICGGMYRACSTWQYEVVSHLIIHYQGGQRLGYLTGEQYAA
                     VFQPATPTSPEAPLRTGYRRVVKTHEGDRSFARALAERRARAVYAYRDVREVVFSLMH
                     KRGMTFERLLRQGMIHQILANDRFWMAQPDVLVQRYDDLVADPVRGVMELARHLGISL
                     EASEARQIASEYSHESNRARTEALRQRLQQAGVDLESTTNAQICDPTTLLHWNHIRGP
                     KSKCWSALASQGQRSVLERLCGRWLRARGYSLVTIDSGHANISLRARLSMEFDLMVGK
                     ANFLIRSASQRWPRTARAIKRILGIPVDAQAGATAWADPTPSRSDARGLNPPHIPITG
                     FSTVDERELVERDMRTS"
     gene            complement(3811..>4644)
                     /locus_tag="VHS97_02065"
     CDS             complement(3811..>4644)
                     /locus_tag="VHS97_02065"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3447006.1"
                     /translation="RYEGQSANFQLAEARESSGQLSEAADLFKKAATESAGKPYPLQA
                     ALIREAEVLAELAMVEPDRVKDAKDKLTRFLQSYPNSRHLAAARATQARLQLHAGDFA
                     TALATIAELEKLATGKERAKVLHTRVLAKQGKFDEAIGKLDNLIADYPKNSPQQRAAL
                     FAKAENLAGKKQFHEAESLLRAVIQSNPPEDAAALAAAYNALGDCLRAANRPKDALFA
                     YLHTDLLYSKDKEEHPRALHQIVEIYRQLKQDGRADDFAQRLKQEYPRSPWAVARTNS
                     Q"
BASE COUNT          959 a         1386 c         1385 g          914 t
ORIGIN      
        1 gcgaatttcc atgctgcgga tgaatccgtc cgcaggcgcg accagcgtca cctgccggat
       61 cggctccagg ctctcggcga cctggtagcg ctcgggcatt gagagctcta ttggcacgga
      121 ctcgatccga gcggcagtcg ggacggtctg accgcaagcc acgcccatcg aggcgaacca
      181 aagggctaaa ccacgcgaca gggccttgcg gcacatggca cacctcgcac ggtcaataat
      241 tcggatcgaa tttcgtagac gctttgaatg ctccctgaac tcacgttaat cccaagggcc
      301 ctgagcgtca acagactgcc acgctgcggc cagcccactc atgacctctg gcgaggcccg
      361 cccgcccgcg ttacgatcgg ttactatgga atgtcgcaac cacacaagcg gtgcgagctt
      421 tcacccgagc atcgatccca agaaaggtga accagatggc gaccgatcga atcacgcggc
      481 gcgatttcgt ggaatcaatg ggcatgtcgg ccggagcaat cagcctactc ggaacgaccg
      541 gggtcgccgc cgcttcgccc aatgataaga ttcggctcgg attgatcggc gcggggagcc
      601 ggggcaatca attgctcgat tcgttcttta aacagtcgga catcgagatc atcgccgttg
      661 ccgacgtgga tgatcgccat gctggcgaaa ccgccgagcg ggtcaagaaa gaaaaaggga
      721 acctccctca gagcgcgcgc gactatcgct tcctgctcga tcgcaaagac ttagacgccg
      781 tgatcatcgc cacgcccgac cactggcacg ctcttcccgc gatccaggcg gtgctggcgg
      841 gaaaggacgt ctatgtagaa aagcccgtcg cgcacaacgt cggcgaaggc caggcaatgt
      901 tgcatgccgc ccgcaagacc aacaagatca tggcggtcgg gacccagcag cgttcttcgt
      961 cccatttcca aagggctgtt gagatcgtgc gctcgggtca gctcggcaag atcttctggg
     1021 tgcagacttg gaacttcgaa aatatcagcc cggtcggcat gggtaagtat cccgacggcg
     1081 aagcaccatc gcatgtcgat tacgatcgtt ggctcggccc cgcgcctctt cgatcgttta
     1141 atccgaaccg gttccacctg ctgtttcgct ggttcttcga ctatgccggc ggcatgatga
     1201 gcgactgggg cgttcacttg aacgacatcg tgatctgggc tctcaattcc aagggtccca
     1261 aatccgtctg tacgaccggt gggatcttca ccaccgacga cgagcgcgac acgccagaca
     1321 ccatgcaggt cgtctacgaa tttccgggat gcacactgac gtactcgatg cgcaagggga
     1381 acggccttaa gtataacggc catgagtacg ggatcctctt ctgcggcacc gacggcagct
     1441 tggtgctcga ccgaagtggc ttcgaggtta ttccggacaa ggtcgtgcta ccgtacggaa
     1501 tcaagctcgt ccacggcgat cgacccaccc gcaagatcga actgaaaccc gagtcggtca
     1561 ctaaaggtgt cgacggccaa gatgcacacg tgcgcaactt cctcgattgc atgaagtctc
     1621 gtgctttgcc gacctgcgat atcgagattg cgcaccggtc gaccaacacg tgccacctgg
     1681 ggaacatctc ctacaagctg ggccgtaagc tcgaatggga cgctgacaac gagacgttca
     1741 aaaacgattc ggaggccaat gcgcttttga agcgagagcc gcgtaaggga tacgagttgc
     1801 cccaggtgtg aggtcgggcg tgtcccaatt gtccgccagc gtcagagcat ccatgaaaaa
     1861 aaccgcgggc catttcttgc gcatctgtgg tttattgatc gagatgctgg gcgtctgggg
     1921 cgtcttcagt tcgaccggtg cgaaaaactc ggcccgactg cagctcccag ggggaaacga
     1981 aatccccctg gcgtggttgg ccgtcggtgt tggttttgtc ctctggttca ccggcacgat
     2041 cctagtctac ttcaccaggc cgacacgaaa gactccgagc cacaggttca gtgaaccgac
     2101 tgacgtacct cctgtccaaa agtcctcctg accccaaagg ccactccaca ttgcccaata
     2161 ctcgccatta acgccgcggc gcccagaagt gctggcatga tgcaaaagac gaaatcagaa
     2221 ataggagccc ggcatctata ctgggcgatc tggcggtttt cggcagatgc cggacgcgct
     2281 cctttttgcc cgcgcatccg ccacacgtta ccatttgaag tgtcgggcac ttattctggg
     2341 taagctgcgt caagtaaatc aacttgcggc cgcgacgatg tgaggccctt gcggcggctg
     2401 aatgcgatag ccgcgatttc gtctggtcta cctttccgag tcaggataga acgacgtttt
     2461 cgagcattgc gctgtcgttt gccaatgcga ggctccggcg caggggcgag acaaccgtcc
     2521 gcgccagacc ggctagaccc gcaaggaggt ctcaccatgg tgaacgagca agatcagaac
     2581 tcgcagaact cgatgcacag actcgaccca cctgggcggt tattccgcct ggaatggaac
     2641 ctgtttcgtg ccgtgcgatc agtagcggcc ggctcagtct cagctggcgg catgccggcg
     2701 aacaccgacg gcggaagcct ggaacgacca ccgagcgacg tcatctgcgg cggaatgtac
     2761 cgcgcttgct cgacttggca atacgaggtg gtatcccatc tgatcatcca ctaccagggt
     2821 ggacagcgac tagggtattt gaccggcgag caatatgcgg ccgtcttcca gcccgcgaca
     2881 ccgacctcgc cagaagcacc actgcgaacc gggtaccggc gtgtcgtcaa aacgcatgag
     2941 ggcgaccgat cgttcgcccg cgcgctggcc gagagacgag cgcgggcggt ttacgcctat
     3001 cgtgacgtgc gcgaggttgt cttttcgctc atgcataaac ggggaatgac gttcgagcga
     3061 ctgctccgcc agggaatgat ccatcagatt ctcgccaatg atcggttctg gatggctcag
     3121 cctgacgtct tggttcagcg ctacgacgac ttggtcgccg acccggtccg gggagtgatg
     3181 gaactcgcgc gtcatctcgg gatctcactc gaagcgagcg aggcacgcca gattgccagc
     3241 gagtactccc acgaatcaaa cagggcacgc accgaggcac tacgacaacg cttgcagcaa
     3301 gccggagtcg atctggaaag cactaccaac gcgcagatct gcgacccgac aaccttgttg
     3361 cactggaacc atattcgcgg gccaaaatcg aagtgctgga gcgcgctggc cagccaagga
     3421 cagcggtcgg ttctggaacg gctttgcggc cgctggctgc gtgcgcgagg ctattcgctc
     3481 gtgactattg attccggcca cgccaacatc tcgcttcgcg cgagactgag catggaattc
     3541 gatctcatgg tgggcaaagc gaatttcttg atacggtcag cgtcacaacg atggccgcga
     3601 accgcacgcg cgatcaagcg gattctcggc attcccgtcg acgcccaggc aggcgccacc
     3661 gcgtgggccg atcccacacc gtcacgttca gatgcgcgag ggctgaatcc cccgcacatt
     3721 ccgatcacgg gattttcgac tgtcgacgag cgagagttgg tcgaacgaga catgcggaca
     3781 tcgtgagtcg gaatcgaatg ctcgatcgtt ttactgcgaa ttcgtgcgcg cgactgccca
     3841 ggggctgcga ggatattcct gcttcaaacg ctgggcaaag tcgtcggcgc ggccatcctg
     3901 cttgagctgg cgataaatct cgacgatctg atggagcgcg cggggatgtt cctccttgtc
     3961 tttgctgtac agcaggtcgg tatgaagata ggcgaaaagc gcgtccttgg gccgattggc
     4021 agcgcggagg cagtctccga gcgcgttgta agcggcggcc agtgcggctg cgtcttccgg
     4081 cgggttcgac tggatcaccg cgcgcagcaa tgattcggct tcatgaaact gcttcttgcc
     4141 ggccagattc tcggccttgg caaacagggc cgcgcgttgt tgaggagaat tcttgggata
     4201 gtcggcgatc agattatcga gcttgccgat ggcctcgtca aacttgcctt gcttggccag
     4261 gaccctggtg tgaaggacct tggctcgttc cttccccgtt gcgagctttt caagctcggc
     4321 gatcgtggct agagcggtgg cgaaatcgcc ggcatggagc tggagccgtg cctgagtagc
     4381 tcgcgccgcc gcgaggtggc gactgttagg atacgactgc aagaaacgcg tgagcttgtc
     4441 tttcgcatcc ttgacgcggt cgggttcgac catggccagt tcggccagga cctcggcctc
     4501 gcgaatgagt gcggcctgaa gcggatacgg cttaccggcc gattccgtgg ccgccttctt
     4561 gaacaaatcg gccgcctcgc tcaactgtcc gctcgattcc ctggcttcgg ctagttgaaa
     4621 attcgccgac tggccctcgt agcg
//
LOCUS       DATAQL010000124         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_929748, whole genome shotgun sequence.
ACCESSION   DATAQL010000124 DATAQL010000000
VERSION     DATAQL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929748"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2101
                     /locus_tag="VHS97_02070"
     CDS             <1..2101
                     /locus_tag="VHS97_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3447007.1"
                     /translation="FVDGEASSLSQFAQRHPELGINKIDTHRSPVIGPRLIGSSANGP
                     NQAVLSIVSLNSMYVPQKTRVAVDRILEYINKETQAPPPGLSRLVTGSAAVGHDTNTA
                     TDESIQNTTNTTIALVILILVAVYRSPLLAMVPLVTIAISLVASLRLIALLTQIPGLG
                     FQVINITQIFIVVVLFGAGTDYCLFLVARYREELGLGKSRVEALRKAIGQMGEALVAS
                     AATVIVGLGMLGFSSFATFRYSGPTIALSLAVALAAALTLAPAMLVWIRAGLFVPATP
                     PQREAGENSETERRDPLTLTSFWMGVADLVVSYPLTIFAACVAVLVPLAVVGARATPN
                     YGQLADLDPDRPSVVGANAARTYFVVGELSPTIALIEHPTLDLRSPQGRAVIEEISRR
                     LAAIASVAEIRSLTRPIGKPEGPATGKSLVARLADRAVRIAAETRYVTTKPRQAADVN
                     HITRLEIVFNSDPFSELGLQTLEDVRATLIRATAAGEPLEGTKRIGLAGSTPAVSDLK
                     RVTTGDQHRMYVLVPLGVYFVLVALLRRPGLSLYLIATVVLTYLASLGLTDLLFHALH
                     RGPGPWEGLDWTVGFFLFVILVAVGEDYNILLMARVIEEERKYGVIEGTRRAVAQTGG
                     IISSCGLIMAGTFGSMLTGSLASLRELGFALGLGVLLDTFLVRPILVPAFVVLIDRVR
                     HRHRAASRPAEIEAGRS"
     gene            complement(2217..2708)
                     /locus_tag="VHS97_02075"
     CDS             complement(2217..2708)
                     /locus_tag="VHS97_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447008.1"
                     /translation="MRGFAIGLALLVAAPGCGSSDRIRVSGKVVRGGAAIACPPDRML
                     GVTLIAIDVKDQEGKGVSAGEPYPAEVNPADSTFVVPGPDGSGILPGRYRVALIQKPT
                     AAAVLAAQEKAGKKKTIRRDTDFFKNQFSAANSPIVRDLGASCDLLIDLDHPTEQKVG
                     DVR"
     gene            complement(2831..3994)
                     /locus_tag="VHS97_02080"
     CDS             complement(2831..3994)
                     /locus_tag="VHS97_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3447009.1"
                     /translation="MERRAFTLIELLVVISIIAVLIALLLPAVQSAREAARRAQCTNN
                     LKQLGLAAQNFVSSNNNLPDGMNEPYIDGLNPNEVSGKFVNDSTNPFGPNWAVLLLPY
                     IEQQNLFNASNVLGYPGIPGPYSPGPPYSSVPNASSYNMDWCNTTVRSTPLNVMTCPT
                     DVNNNPSNFYFSNQSDAAYGITPVDPRTGTPLMNWARGNYGAVEGGTDGDHVVNGYGG
                     ESADPFPGTSKRGVMGNNFGVTLAQVTDGLSNTMFFGELRAGLNSLDIRGTWAIGMPG
                     ASLCCHAKNYNPTPNGTFMISPPNCGDGGDELQGCYAFAPMFPNRGQLGMPCSCQQGH
                     RNTGGQSRSMHPGVVNVGMGDGSVRFIKNTIANQVWFELLVSNDGWILGSDQY"
BASE COUNT          718 a         1301 c         1369 g          869 t
ORIGIN      
        1 ttttgtggat ggcgaggcct cgagcctctc ccagttcgcc cagaggcatc cggaactcgg
       61 gatcaataag atcgacactc accgctcgcc cgtgattggt ccacggttga ttggttcgag
      121 cgccaatggt cccaatcagg cggtgttgtc gatcgtttcg ctgaacagca tgtatgtccc
      181 ccagaagacg cgcgtcgcgg tcgaccggat cttggaatac atcaacaaag agacccaagc
      241 gcctccaccg ggactcagtc gcttggtaac gggctcggcg gccgtgggac acgacaccaa
      301 cacggcgacc gacgaaagca tccagaacac gacgaacacc acaatcgccc tggtgatctt
      361 gattctggtg gcagtttacc gttcaccgct cctggcgatg gtgcccctgg tcacgattgc
      421 catctcgttg gtcgcctcgc tcaggctgat cgcgctgctc acgcaaattc cgggcctggg
      481 atttcaggtg atcaacatca cgcagatctt cattgtggtc gtgttattcg gcgcagggac
      541 tgactactgt ctcttcctgg tggcgcggta tcgggaggag ctcgggctcg ggaaatcgcg
      601 ggttgaggcg ctccgtaaag cgatcgggca gatgggtgag gcgctggtcg ccagtgccgc
      661 gacggtgatc gtcgggctgg ggatgctcgg tttctcgagt tttgccacgt tccggtacag
      721 cggaccgacg atcgcgctaa gtcttgcggt ggcgttggcg gccgccctga cgttggctcc
      781 cgcaatgctg gtctggattc gcgccgggct tttcgtgccc gccacgcctc cccagcgcga
      841 ggccggtgag aattccgaga cagagcgtcg tgacccgctc accctgacga gcttctggat
      901 gggggtcgct gacctggtgg tgagctatcc cttgacgatc ttcgcagcct gcgtggcggt
      961 cctagttccc ttggccgtcg ttggtgcgcg cgccacgccg aattacggcc agctcgccga
     1021 ccttgacccg gatcgcccga gcgtggtcgg tgccaatgcg gcccggacct acttcgtcgt
     1081 tggcgagttg agtcctacca tagccctgat cgagcacccc actctggact tgcgctcgcc
     1141 gcaaggccgg gccgtgatcg aggaaatcag ccgccggctg gcggctattg ccagtgttgc
     1201 cgagatccgc tcgttgacgc ggcccatcgg caagccggag ggacctgcca ccggcaagag
     1261 cctggtcgcc cggttggccg accgggccgt gcgcattgcc gccgagaccc gatatgtgac
     1321 caccaagcca aggcaagcgg ccgatgtcaa ccacatcacc cgcctcgaga tcgtttttaa
     1381 ctccgatccg ttctcggaat tgggcctcca aacactggaa gacgtccgcg ccacgttaat
     1441 acgcgccacc gcggctggcg agcccctcga aggcaccaag aggatcggac tagcgggttc
     1501 caccccggcg gtcagtgatc tcaagcgcgt gaccacgggc gaccagcacc ggatgtacgt
     1561 cctggttcca ctcggggttt actttgtttt ggtcgccttg ctacgccggc ctggtctaag
     1621 cctctatctc atcgcaacgg ttgtactgac ctaccttgcc tcattgggat tgaccgactt
     1681 gttgtttcac gccctgcacc ggggccccgg gccctgggag gggctcgact ggaccgtggg
     1741 cttcttcctg ttcgtgatct tggtggcggt gggcgaggat tacaatattc tgcttatggc
     1801 ccgcgtgatc gaggaagagc gaaagtacgg cgtcatcgag gggacacgca gggcagtcgc
     1861 tcagaccggc ggtatcatca gctcgtgcgg tctgatcatg gccggaacct ttggttccat
     1921 gctgacggga agtttggcct ccctccgcga gctgggtttt gcgctggggc tgggcgtcct
     1981 gctcgacacg ttcctcgttc ggccgatcct ggttccggcc ttcgtcgttc tgatcgatcg
     2041 agtccggcat cggcaccgcg ctgcgtcccg tccggcggaa attgaagccg ggcgaagtta
     2101 ggtatggttt cgtccgagcc agtggacctg cccgctacgc ccgttgaagc cctcgcaagc
     2161 ggcaatcttc attaactcaa agccgaggct gagggcggcc gaccgggttt ttcgcctcac
     2221 cggacgtcgc cgactttctg ctcggtcgga tggtccaagt cgatgagcag atcgcacgag
     2281 gcccccaggt cccggacgat cggcgagttc gcggcgctga actggttctt gaagaagtcg
     2341 gtatcacgac ggatggtctt tttcttaccc gccttctcct gggcggccaa taccgcggcc
     2401 gccgtcggct tttgaatgag cgcgacgcgg taccggcccg ggaggattcc gctcccgtcc
     2461 gggcccggca cgacgaacgt gctgtccgcc gggttcacct cggcggggta gggctccccg
     2521 gcggagacgc ccttcccctc ctggtccttg acgtcgatcg cgatcagcgt gacaccgagc
     2581 atccggtccg ggggacaggc gatcgcggcg ccgcccctga cgaccttccc gctgacccgg
     2641 atgcggtccg acgaaccgca gccgggcgcg gccacgagga gggccagacc aatggcgaac
     2701 ccgcgcaagt ctatcataat tcccataacg tgatagtatc ctaatctgtc ggtcggttcg
     2761 gggatcacgt ctcggcaacg tggaggggtt tgacccgacc gaggggttcg gcttgcggcc
     2821 tcgagtgcgg tcagtactgg tctgagccca ggatccagcc gtcgttgctg accaagagct
     2881 cgaaccaaac ctgattggcg atcgtgttct tgatgaatcg cacgctgccg tcgcccatgc
     2941 cgacgttgac cactccgggg tgcatgctgc gggactggcc tccggtgttg cggtgcccct
     3001 gctggcagct gcagggcatg ccgagctgcc cgcggttggg gaacatcggg gcaaaggcgt
     3061 agcacccctg cagctcgtcg ccgccgtcgc cgcagttagg cggggagatc atgaacgtcc
     3121 cgttcggcgt cggattgtag ttcttggcgt ggcagcagag gctggcgccg ggcattccga
     3181 tggcccaggt gccccggatg tccagggagt tgagccccgc cctcagctct ccgaaaaaca
     3241 tcgtgttcga caggccatcg gtaacctggg cgagcgtcac gccgaagttg ttgcccatca
     3301 cgccgcgctt cgacgtgccg gggaacgggt cggcactctc gccgccgtag ccgttgacga
     3361 cgtggtcgcc gtcggtcccc ccctcgaccg caccgtaatt gccgcgtgcc cagttcatca
     3421 gtggagttcc cgtcctcggg tcgacggggg tgatgccgta ggccgcgtcg ctctggttgc
     3481 tgaagtaaaa gttactcgga ttgttgttca cgtcggtcgg acaggtcatc acgttcagcg
     3541 gggtcgagcg gactgtcgta ttgcaccagt ccatgttgta agacgatgcg ttcggtaccg
     3601 agctgtaggg cggacccgga gaataggggc cggggatgcc ggggtaaccc aggacgttcg
     3661 acgcgttgaa caggttctgc tgctcaatgt aaggcaggag caacaccgcc cagttgggcc
     3721 cgaacgggtt ggtcgagtcg ttgacgaact tgccgctgac ttcgttcgga ttgagcccgt
     3781 cgatgtacgg ctcgttcatc ccgtcgggaa ggttgttgtt actcgagacg aagttctgag
     3841 cggccaggcc gagctgcttg aggttgttgg tgcactgggc gcggcgggcc gcctcgcggg
     3901 ccgactggac cgcgggtaag agcagggcga tcaacacagc gatgatcgag atcacgacca
     3961 gcagctcgat cagggtgaaa gcacgtcgtt ccatcgcagt ctccagtgag cggtttgaac
     4021 acaggcgacg accgggactg cggattatat gcgcctccgc gaccgcgcat caaaacaaga
     4081 ttcaagtatc tttttgtgaa gaatctgtta attaaaaatt ctttatttta gctcgtcatc
     4141 acatcagggg gggatggcgc gggcccaatc acgcatcgac aatcgcgtgc agggacgcaa
     4201 gcgggaatgt ccgacacttt ccgaacctat tctttggtcc gagcgggtgg cggcgcg
//
LOCUS       DATAQL010000125         7445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3677952, whole genome shotgun sequence.
ACCESSION   DATAQL010000125 DATAQL010000000
VERSION     DATAQL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3677952"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1325
                     /locus_tag="VHS97_02085"
     CDS             255..1325
                     /locus_tag="VHS97_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3447010.1"
                     /translation="MSSRRAFTLIELLVVIAIIAVLIALLLPAVQAAREAARRTQCVN
                     NLKQIGLALHNYHSVNDCFPMGSGSGVLTYPPPTYQAKECWAVHGAILQFLEQSPTYN
                     AINFYWSPDEPPNQTVDNTQILTYLCPSDPNATSNTLGDNGVSTTGNNCYFASIGPTT
                     DFRVGLATSAPSFGANPTAGLFAFQQSKSIARVIDGTSNSIAFAESTVGVPGQTAKQK
                     LIGLVNVAIPALALQANAATNVTGVQAGIAACSAAWQAGSSSAVDLQRGDSWQQGAMC
                     MTMFNTICTPNDQADEWAYCSNSGSGACSNYSNADSYHPGGVNVLMGDGSVKFIKNSI
                     NQLTWWALGSIAGGEVISSDSY"
     gene            1361..3934
                     /locus_tag="VHS97_02090"
     CDS             1361..3934
                     /locus_tag="VHS97_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3447011.1"
                     /translation="MTTFAYAAARRRKLRGLFEGLDEGSPSLSNKQCYPAIEIGICYR
                     NVPQGSTLRYKNCTEVELTKRTHGMMGRVEVTPRVLTLTRSRPPYSAASTVALATAVI
                     VGHWGARAEDSPGLRTFREAIEPVLVTECYRCHSAKAEKIKGGLRLDSREAMLRGGDT
                     GPAVVPGKSGESLLVQALRHEDGLEMPPKAPALSGEIVARFVQWIDAGAPAPDVGSAT
                     AVVAPGPGDARQHWAFQPITRPEPPAVNDPGWALTPVDAFVLAKLEERGWSPAADASR
                     SDWLRRVTFDLTGLPPSPEDVEAFERDASANAFESVVDRLLASPRYGERWAQHWLDVI
                     RYAETEGFEYDRHIPDAWRFRDYVIGSLNRDKPFNRFVTEQIAGDEIAPGDQEAATAS
                     AFHRLGPVRRNAGNPDIALSRNEVLTERTDIVGTAFLGLTVGCARCHNHKLEPISQKD
                     YYRLQAYLAATNESDVLLAPDKERTAWEAETKSVNKRMEQVRARMKRVQGPDRERLVK
                     ELQALEDKLPAPLPTIPATRNDSEHRTPIHVLKRGEWEAKGEPVGPRPPSILVADQEP
                     ELPADLADPRTRLASWLTGPTQPLTARVFVNRVWQHHFGAGLVKTVNDFGTKGDRPSH
                     PELLDWLTSSFISSGWRLKPLHRMIVLSHAYRQSDHALSSAEAATADPENRLLWHFAR
                     RRLTAEEIRDTMLVASGRLNDKAGGPSVMVPVDSDMIRLLYKPAQWKVATDATEHDRR
                     SVYLLAKRNLRPPFLEVFDSPALMTSCARRESSTHAPQALELLNGRLSNDLASSFALR
                     LQAECGGDVSKVVSRAFALALGRAPTARERALALEFLREQPLTEFALAVFNLNEFLYV
                     R"
     gene            3943..5394
                     /locus_tag="VHS97_02095"
     CDS             3943..5394
                     /locus_tag="VHS97_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447012.1"
                     /translation="MSLDTGMFAAMYPHSRPTRTRREFVRDACCGFGGLALASILHEE
                     AVRAAKVTDPLDPKPPHLSAKAKSVIFLYMAGGPSHLETFDPKPLLNRLEGQTRPKEF
                     GEAKYQFVQRDARLLGTRRQFKKYGQSGMEISDLFPETARCADDLAVIRSCHGDMVVH
                     SAAQYELFTGRVTPGFPSMGSWVLYGLGSESQSLPAYVVMPDPKGALEAGQPMYANGF
                     LPAVYQPTMFRGGDRPVLNLDLPTGVAHRARTRTLKLIREMNESTLDPADLEFSARVN
                     AYDLAFKMQTEAPEVLDLSREPRGTMDLYGIGREPTNDYGRRCLLARRLVENGVRFVC
                     VVSGGGAGNQQWDAHSNIEENHLRKAAETDKPVAALLTDLKRRGLLDTTLVVWGGEFG
                     RSPEAESGKGRDHHNLGFTMWMAGGGVKGGTVVGATDAIGLHAVDRPYHFRDIHTTIL
                     HQLGLDQHKLTYPHLGRDERLTFVEGKVVEEIV"
     gene            complement(5398..6699)
                     /locus_tag="VHS97_02100"
     CDS             complement(5398..6699)
                     /locus_tag="VHS97_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3447013.1"
                     /translation="MTTGTGVGFGSRRSFLDRACRGITGGLLTSGISARHLWARAGDE
                     SADSLRIVKVEPHLLTGVRGYGPWLLVRVETADGFVGWGEGTNFPGVQPIAAAVRSLS
                     DVVVGARAWDIEQLWNRMYRSLYYNGMGGVVMAAISGIDTALYDIVGKKLGVPVYNLL
                     GGKVRETLRIYANGWTEGIPHTPEALAKRTKELVDKGFTGCKFDPFSTSPFNREVTQE
                     DLRGAVAQVEAVRSAGGPDYEIAIDVHGRWNTNSALKIIRALEPYRLFFLEEAVPPEN
                     IAAMAEVQRAVTVPLATGERIFGRHGFRELLERQAVRIVQPDIARTGGITEFKKIASM
                     ADTYYVSVAPHNPNGPICTIASIHACLSIPNFLVLEFFEPDEPVFREIIDGGLRRDKA
                     AVYPPSGPGLGVRISDEVLRKHRFDEARTREMELRTFNTMK"
BASE COUNT         1373 a         2458 c         2272 g         1342 t
ORIGIN      
        1 ccgataggtg gcacatcttt tgcagcgcct ttgtattcag cattgatgtt aagcaagtgg
       61 atgctctttg tttcaaaata tttgttgatt gtaagaaccg cttgccgcgt tcttgattct
      121 cgtccatctt ttcgctctgc gacgcaatca acagtggttc ctgttttcac gaccggccgt
      181 ggcgacctct cccgccatgt acgagcgacc gcgatcgatc gttccgtcca atcctcgcac
      241 tcgggagatc cgacatgagt tcacgccgcg cgttcacctt gatcgaattg ttggtagtca
      301 tcgcgattat tgcggtgctg atcgctctct tacttcccgc cgttcaggcc gcgcgcgagg
      361 cggccaggcg gacgcagtgc gtcaacaacc tgaagcagat cggcctggcg ctgcacaact
      421 accacagcgt caacgactgc ttccccatgg gctccggcag cggcgtcctt acatatccgc
      481 cgcccaccta tcaggcaaag gagtgctggg ccgtgcacgg ggccattctc cagttcctcg
      541 agcagtcgcc gacctataac gcaatcaatt tctattggag ccctgatgag ccccctaacc
      601 agacggtgga taatacgcag atcttgacct acctctgccc atccgatccc aacgcaacct
      661 cgaacacgct tggcgacaac ggggtgtcga ccacgggcaa caactgctac ttcgcgtcga
      721 tcgggccaac gacggacttc cgcgtgggcc tcgcgacttc ggcgcccagc ttcggcgcga
      781 acccgacagc tgggttgttc gctttccaac agtccaagtc gatcgcccgg gtgatcgatg
      841 ggacgtcgaa ctcgatcgcc tttgccgagt cgactgtcgg ggttcccggc cagactgcca
      901 agcagaagct gatcggcctt gtcaacgtcg cgattccggc cctggccctc caggcgaacg
      961 cggccaccaa cgtcaccggc gtgcaagcgg gaatcgccgc ctgctcggcg gcctggcagg
     1021 cgggctcaag ctcggctgtc gaccttcagc ggggtgattc gtggcagcag ggggcgatgt
     1081 gcatgacgat gttcaatacc atttgcaccc ccaacgacca ggccgatgaa tgggcctatt
     1141 gcagcaactc cggttctggg gcttgttcca attacagcaa cgccgacagc taccaccctg
     1201 gcggcgtcaa cgtgctgatg ggcgacggca gcgtcaagtt catcaagaac tccatcaacc
     1261 agctaacgtg gtgggcattg ggttcgatcg ccgggggtga ggtgatcagc agcgactcct
     1321 attaacgtgc cgggcgcttt tccggcactc gaaggaatgg atgaccacat ttgcgtatgc
     1381 agccgcccgc cgccgaaaac tgagagggtt attcgagggg cttgacgagg gaagtccctc
     1441 gctgtccaat aagcaatgct accccgccat agaaattggc atttgttatc gtaacgtccc
     1501 gcaaggatcc acgttgcgat acaagaattg cacagaggtc gagctaacca aacgaacgca
     1561 cggtatgatg ggccgcgtgg aggtaacccc aagagtgctc acgctgactc gctcccgccc
     1621 gccctacagc gccgcatcga cggtcgcact tgcgacggcc gttatcgtgg gccactgggg
     1681 cgcccgcgct gaagactctc ctggactgcg cacctttcgg gaggcgattg agccggtcct
     1741 cgtcacggag tgttaccgct gccattctgc gaaggcagag aagataaagg ggggccttcg
     1801 actcgactcg cgcgaggcca tgttacgcgg aggggacacc ggcccggccg ttgtcccggg
     1861 caagagcggt gagagtttgc tcgtgcaagc cctccggcac gaagacggac tggaaatgcc
     1921 ccccaaggcg cctgcgctca gcggggagat cgtcgcccga ttcgtccagt ggatcgacgc
     1981 cggcgcaccg gcccccgacg ttggctcggc cacggcggtg gtcgcgccgg gacctggcga
     2041 cgcgcgccag cactgggcgt tccagccgat cacgcggcct gagccaccgg cggtgaatga
     2101 cccggggtgg gcccttacgc ccgtcgacgc gttcgtcctg gccaagcttg aagagcgcgg
     2161 ctggtccccc gcggccgacg ccagccggag cgactggctg cgccgcgtca cgttcgactt
     2221 gactggcctg cccccctcgc ccgaggatgt cgaggcgttc gagcgcgacg catccgcgaa
     2281 cgcatttgag tccgtcgtcg atcgcttact ggcaagcccg cgctacggcg agcgctgggc
     2341 gcagcactgg ctcgatgtga tacggtacgc cgagactgag ggattcgagt acgaccgcca
     2401 tatccccgac gcctggcggt tccgcgacta cgtcattggc tcattgaacc gtgacaagcc
     2461 gttcaaccgg ttcgttaccg agcagatcgc cggggacgag atcgcgccgg gcgaccagga
     2521 ggccgcgacg gcgtcggcgt tccatcgact cggcccggtc cgccgcaatg caggaaaccc
     2581 cgacatcgca cttagccgca acgaggttct gaccgagcgc accgacatcg tcggcaccgc
     2641 ctttctcggt ctgacagtcg gctgtgcccg ttgccacaac cacaagctcg aaccgatcag
     2701 ccagaaggat tactaccggt tgcaggccta tctcgccgcg accaacgagt ccgacgtctt
     2761 gctcgctcca gacaaagagc ggaccgcctg ggaggccgag accaagtcgg tcaataaacg
     2821 gatggagcag gtccgcgccc ggatgaaaag agtccagggc ccggatcggg agcgactagt
     2881 caaagagctc caggcgctgg aagacaagtt gcccgcgccg ttgccgacga tcccggccac
     2941 gcggaacgac tccgagcacc ggacccccat ccatgtccta aagcgcggcg agtgggaggc
     3001 gaagggcgaa cccgtcggcc cgcggccgcc gagcattctc gtcgccgacc aagagcccga
     3061 gttgcccgcg gacttggccg acccgcgcac gcgcttggcc agttggttga ctggcccgac
     3121 acagccgtta acggcccggg tcttcgtcaa ccgggtctgg cagcatcact tcggtgccgg
     3181 gctggtcaag acggtcaacg acttcggcac caagggcgac cgccccagcc accccgaact
     3241 gctcgactgg ctaacctcat cgttcatctc cagcggctgg cggctgaagc cgctgcaccg
     3301 aatgatcgtt ctgagccacg cttaccgtca gagcgatcac gccctttcat ccgccgaggc
     3361 cgcgacggcc gatccggaga accgcttgct ctggcatttc gcccgccgcc ggctcacggc
     3421 cgaggagatc cgcgatacca tgctcgtcgc ctccggccga ttgaacgaca aggccggcgg
     3481 cccgagcgtc atggtgccgg tcgactccga catgatccgc ctcctgtaca agcccgcgca
     3541 gtggaaggtc gccactgacg cgaccgagca cgatcggcgc tcggtctacc tgctcgccaa
     3601 gcgcaatctt cgccctccgt tcctggaagt gtttgactcg ccggccctaa tgaccagttg
     3661 cgcccgccgc gagtcgagca cccacgcgcc tcaggcgctc gaactcttga acggccggct
     3721 ctcgaacgac ctcgcctcgt cgtttgcgct acggctccaa gccgagtgcg gcggcgatgt
     3781 gtcaaaggtc gtcagtcggg cattcgccct tgccctcggc cgcgcaccaa cggcccggga
     3841 acgggccctg gcgctcgagt tcctgcgcga gcagccgttg acggaattcg cgttggccgt
     3901 gttcaatctg aacgaattct tgtacgttcg ctgatcacca aaatgtcgct cgacaccgga
     3961 atgttcgctg ccatgtaccc tcactcccgc ccgacgcgca ctcgccgcga attcgtccgt
     4021 gacgcctgct gtggatttgg tggcctggcc ttggcatcga tcctccacga ggaggcggtt
     4081 cgggccgcca aggtcactga cccgctcgac cccaagccgc cgcacctgtc cgcgaaggcg
     4141 aagtcggtca tcttcctgta catggccggc gggccgagcc acctggagac gttcgacccc
     4201 aagccgcttt tgaacagact cgagggccag acgcgtccca aggagtttgg cgaggccaag
     4261 tatcagttcg tccagcgcga cgcccgcctg ctcggcacgc ggcggcagtt caaaaagtac
     4321 ggccagagcg ggatggaaat ctccgacctc ttcccggaga ctgcccgttg cgccgacgac
     4381 ttggccgtaa tccgctcgtg ccacggggac atggtcgtcc actcggcggc ccagtacgaa
     4441 ctcttcaccg gcagggtcac gcctggcttc ccgagcatgg gctcatgggt cctctacggc
     4501 ctgggttccg aaagtcagtc gctgccggcc tacgtggtga tgcccgatcc gaaaggcgcg
     4561 ctcgaagccg gccagccgat gtacgccaac ggctttttgc cggccgtgta ccaaccgacg
     4621 atgttccgcg ggggcgacag gccggtcctc aacctcgact tgcccacggg cgttgctcac
     4681 agagcgcgga cgcgcacttt gaaactgatt cgcgagatga acgagtcgac gctcgacccg
     4741 gccgacctcg aattctccgc ccgggtcaac gcgtacgacc tggcgttcaa gatgcagacc
     4801 gaggctccgg aagtcctcga cctctcgcgc gagccccgcg ggacgatgga cctctacggt
     4861 atcggccgcg agccgacaaa cgactacggc cgccgctgcc ttttggcgcg gcggctcgtc
     4921 gagaacggcg tccggttcgt ttgtgttgtc tctggcggcg gcgcgggcaa ccagcagtgg
     4981 gacgcccact ccaacatcga agagaatcac ctccgcaagg ccgccgagac cgacaagccc
     5041 gtcgccgcct tgcttaccga cctgaagcgc cggggcctgc tcgacacgac gctcgtcgtc
     5101 tggggcggcg agttcggccg ctctcccgag gctgaatcag gcaagggccg cgaccaccac
     5161 aacctcggct tcaccatgtg gatggccggc ggcggcgtca aagggggtac ggtcgtcggc
     5221 gccaccgacg ccatcggcct ccacgcggtc gaccgtcctt atcatttccg cgatattcat
     5281 acgacgatcc ttcatcagtt agggctcgac cagcacaagc tcacgtaccc gcacctcggg
     5341 cgggacgaac ggctgacctt cgtggagggg aaggtggtcg aggagatcgt ctgatgccta
     5401 tttcatcgtg ttgaacgtcc gcagttccat ctcgcgtgtc cgcgcctcgt cgaagcggtg
     5461 tttcctgagg acttcgtcgg aaatcctcac tcccaatccc gggccggacg gtgggtagac
     5521 tgccgccttg tcccgacgca gcccgccgtc gatgatctcg cgaaagacgg gctcgtcggg
     5581 ctcgaagaac tcgaggacga ggaagttcgg gatcgagagg cacgcgtgga tcgaggcaat
     5641 ggtacaaatc ggcccgttcg ggttgtgggg ggcgaccgag acgtaatagg tgtccgccat
     5701 cgatgcgatc ttcttgaact ccgtgatgcc gccggtgcgg gcgatgtcgg gctggacgat
     5761 gcgcacggcc tggcgctcga ggagttctcg gaagccatgc cgcccgaaga tgcgttcccc
     5821 cgtggccagc gggacggtta ccgctcgctg cacttccgcc atggccgcga tgttctccgg
     5881 cggcaccgcc tcctcgagga agaacaggcg atagggctcg agcgcgcgaa tgatcttgag
     5941 tgcggagttg gtgttccacc ggccgtgtac gtcgatggcg atctcgtaat ccggcccgcc
     6001 cgccgaacga accgcctcga cctgcgccac ggcgccgcgc agatcctctt gcgtgacctc
     6061 gcgattaaag ggggatgtgc taaacgggtc gaacttgcaa ccggtgaatc ccttgtctac
     6121 caactcttta gtgcgtttgg ccagggcctc gggcgtgtgt ggaatgccct cggtccaacc
     6181 gttggcgtag atccgcagcg tctcgcggac tttgccgcca agaaggttgt agaccggcac
     6241 tccgagcttc ttgccaacga tgtcgtagag ggcggtgtcg attccgctga tcgcggccat
     6301 cacgacgcct cccattccgt tgtagtagag ggagcgatac attcggttcc acaactgttc
     6361 gatgtcccag gccctggcac cgacaacgac gtcgctcagg ctccggacgg cggcggcgat
     6421 cggttggacg ccggggaagt tggtgccttc gccccagccg acgaagccat ctgccgtctc
     6481 gacgcgcacg agcagccacg gcccgtaacc cctcacacca gtgagcaggt gcggttcgac
     6541 cttgacgatc cggagcgagt cggccgattc gtcgcccgca cgggcccata agtgtcgggc
     6601 cgagatacca gaagtgagca gccccccggt gatcccccgg caggcccggt cgaggaacga
     6661 tcgccgtgaa ccgaaaccga cacctgtgcc cgtcgtcatt tggatgcccc ttccccgtcc
     6721 cggatgtgac tcgatgatgc ccgcccacaa tagggtaccg cccatattgc ggccgtaaca
     6781 tcgttgacgc ccccggctca gcggggcagt ggacctggcg gcgcttaatt acgtactgtc
     6841 cggacagtat gacgctggcc cactcgatcg ggggatgtgg tgaaaatggt ctgggatgtg
     6901 gcgtcatacg cgacgtggaa agagtccgga ggctacggag tggggtcagg ccgagccccc
     6961 gtcacgagag atgatgcggc ctgattcgga ccggaggctt ggagcaggct atctgattgc
     7021 atccctgtga ccgtctttgg caaggtcacg atatcgccaa ccagagaaag cggaagatcg
     7081 gcagcgtaca acaagttgcc gggagccttc tctgactgca gccattcaat gtcccgacgg
     7141 accccgccga acggtcttga gacctcttgt cccgacgggc ccggcgatgg gagtcgccga
     7201 ccctcgacat ttgccatcgt tccacaacca aatgatgcta caacagcgga taaagctaaa
     7261 cagcccacaa ctctctgaat taacatattc ggctccccga ccgcccgtca cattccgctc
     7321 actccaaaaa acgccggtga ttatgacggc catccggaat accacaagag gaacatgagt
     7381 caacccgcag acgggcatac gcgcctacgt cggtccggcg aaccgtcgct gacggttttc
     7441 atttc
//
LOCUS       DATAQL010000126         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_929885, whole genome shotgun sequence.
ACCESSION   DATAQL010000126 DATAQL010000000
VERSION     DATAQL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929885"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VHS97_02105"
     CDS             complement(<1..1208)
                     /locus_tag="VHS97_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447014.1"
                     /translation="MTTAPRRISVGFLGTGYIADWHATALRTVKGAGLAAVCDRDLGR
                     ARAFAARYGITRVYDSLGTMLKEGGLGAIHVLVPPELHAQTAREIIDAGLDVLLEKPM
                     AVAANECDELIERARSAGVKVGVGHNFLFAPNYERLKDDVRSGRLGRLDDVTITWNKG
                     LGQLQSGPFDLWMLRDPANIMLEVGSHSVAHMLDLVGLVPILGVQATNRLDLPGGARF
                     FRRWRVQAGPASVGVTLSLSFTPGFSEHMIHVRGSLAAASADFERNTYLLQRHTPAGL
                     DFDRYHMTIADAKALKQQARGTLGQVIHSKLRPNGGSPYGQSIARALQSFYTGSAESL
                     DPRLSPQFGRDVVRTCVEIGRLAPRIQVTEPVRSDNLMAPLANGTAMSGTTSSSREIL
                     VLGATGFIGR"
     gene            complement(1205..2260)
                     /locus_tag="VHS97_02110"
     CDS             complement(1205..2260)
                     /locus_tag="VHS97_02110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3447015.1"
                     /translation="MSQLGIVTIGRNEGERLRRCLTSVAGRDLPVVYVDSNSTDKSVE
                     LARSLGAEIVELNPSLSFTAARARNAGFERLEQIAPGVEYVQVVDGDCEVAAGWLDRA
                     RSVLEERPDVAVVFGRRRERYRDQTIYNRMADLEWDVPVGEAKACGGDAMILAEAFRR
                     VGGYNPSIIAAEDDELCLRIRGQGWKVLRIDADMTLHDMAMTRFAQWWTRAVRCGHAY
                     AEGVARYGRTPERHFVRQMRSTIFWGLLLPLIVVGLAWPTHGASLVLLCGYLVLFWRS
                     ERFFRIGRGWRAADARLYAAFCVLAKFPHVAGMVKYWSRRIQGKPVQIIEYRGAQTVL
                     AAPRPELSPITCDDNLR"
     gene            complement(2223..>2942)
                     /locus_tag="VHS97_02115"
     CDS             complement(2223..>2942)
                     /locus_tag="VHS97_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3447016.1"
                     /translation="ALVDFLEHHPAVGIAGSRLVNREGTAEWSAFRFPTVLGELEGGV
                     RLGLVTRILSRWVISSPAPLVASRCDWSSGASLMVRRAVFEAIGLLDENYFMYFEEVD
                     FCRRAHGAGWAAWYVPQSEVVHLAGQSSGVTGRQRERKRLPGYWFQARRYYNKKHLGP
                     VRAALADLVWSLGFSSYRVRQWLFRKPDTDPESMLRDFVHYNFFPNGSLSIKRSDDRE
                     LPRSWGDRDHESARNRHDRPQ"
BASE COUNT          599 a          958 c          905 g          480 t
ORIGIN      
        1 tcccggccaa tgaatccggt cgcacccagg accagaatct cgcgcgaaga gcttgttgtt
       61 cccgacatgg ccgtgccgtt ggccaagggc gccatcaaat tgtccgaccg caccggctct
      121 gttacctgga tgcgcggcgc aagtctgccg atctccacgc aagtgcgaac cacgtcgcgc
      181 ccgaactggg gtgaaagacg tgggtcgagc gattcggcgg agccggtgta aaacgattgc
      241 aacgcccggg caatactctg gccataggga ctacctccgt tgggccggag cttggaatgg
      301 atcacctggc ccagcgtgcc ccgcgcctgc tgcttcaagg ccttggcgtc cgcaatagtc
      361 atgtggtacc ggtcgaagtc caaacccgcc ggagtatgtc gctgcaggag ataggtgttg
      421 cgctcgaagt cggcggaggc agcggccagg ctgccgcgaa cgtggatcat gtgctcggaa
      481 aaacctgggg tgaaagacaa gctcaatgtc acacccacgg acgcggggcc ggcctgcacg
      541 cgccagcggc ggaaaaaccg cgccccgccg ggcagatcca ggcggtttgt cgcctgcact
      601 ccgaggatcg ggaccaggcc gaccaggtcg agcatgtggg ccaccgaatg cgaccccacc
      661 tcgagcatga tgttcgccgg atcgcggagc atccagagat caaagggacc agactggagc
      721 tggcccaaac ccttgttcca ggtgatcgta acgtcgtcga gccgccctaa ccgtcccgat
      781 cgcacgtcgt ccttcaatcg ctcgtagttg ggcgcgaaca gaaaattatg accgacgccg
      841 accttgaccc cggcggaccg ggcgcgctcg atcaattcgt cgcactcgtt ggcggcgaca
      901 gccatcggtt tctcaagcag tacatctagt ccggcgtcga tgatctcgcg agcggtttgg
      961 gcgtgcagct cgggggggac cagaacgtgg atggcgccta acccaccctc ttttagcatc
     1021 gtgcccagcg aatcgtagac gcgggtgata ccgtagcgcg cggcgaacgc ccgcgcacgg
     1081 cccaggtcac ggtcacaaac ggcagccaga ccagccccct tgaccgtgcg caaggccgtg
     1141 gcgtgccagt cggcaatgta gccggttccc agaaatccta cgctgattcg tcgcggagcg
     1201 gttgtcatcg gagattgtca tcacaggtga tcgggctcaa ctctggccgc ggtgcagcca
     1261 gcacagtctg ggccccgcga tattcgatga tctggaccgg cttgccctgg atccgccgcg
     1321 accagtactt caccataccg gccacgtgag gaaacttggc caggacacaa aacgcggcat
     1381 aaagccgcgc atcggccgcg cgccagccgc ggcctattcg aaaaaaacgt tcgctccgcc
     1441 agaacaagac caggtaaccg cacaagagga cgaggctcgc gccgtgggtc ggccaggcca
     1501 ggccaacgac gatgagcggc aagagaagtc cccaaaagat cgtgctccgc atctgacgga
     1561 cgaaatgacg ctcgggcgta cggccgtagc gtgccacccc ctcggcgtac gcatgtccgc
     1621 accggacagc gcgcgtccac cattgcgcga accgggtcat ggccatatcg tgcaacgtca
     1681 tgtcggcgtc gattcgcaga accttccagc cctgtccgcg tatccgcagg cagagttcat
     1741 cgtcctcggc agcgatgatc gacggattgt agccgccgac acgacggaac gcctcggcga
     1801 ggatcatggc gtcgccgccg cacgctttgg cctctccgac agggacgtcc cactcgagat
     1861 cggccatgcg gttgtagatc gtctgatcgc ggtagcgctc gcggcgccgg ccgaagacca
     1921 cagcgacgtc gggcctctcc tcgagcaccg agcgggcgcg gtccagccaa ccagccgcca
     1981 cttcgcaatc gccgtcgaca acctggacgt attcgacgcc cggggcgatc tgttcgagcc
     2041 gctcgaagcc ggcatttcgc gcgcgggctg ccgtgaacga gagcgacggg ttcagctcca
     2101 cgatctccgc ccccaacgac ctcgccaact cgacgctttt gtccgttgaa ttcgaatcga
     2161 cgtagacgac cggcaggtcg cgcccagcca ccgaggtcag acaccggcgc agtcgttccc
     2221 cttcattgcg gccgatcgtg acgattccta gctgactcat gatctctgtc tccccaagat
     2281 ctgggcaatt cacgatcgtc ggagcgcttg atcgagaggg agccgttcgg gaagaagttg
     2341 taatggacga agtctcgcaa cattgactcc gggtcggtgt caggcttgcg gaaaagccat
     2401 tgccggaccc ggtatgaaga gaatccgagc gaccagacga ggtcggcaag cgcggcgcga
     2461 accggcccca ggtgcttttt gttataatag cgccgggctt ggaaccagta gccggggaga
     2521 cgcttgcgtt ctcgttgtct acccgtcacg cccgaacttt gaccagcaag gtgaacgact
     2581 tccgactgcg gtacgtacca ggcggcccag ccggcgccgt gggcgcgacg gcagaaatcc
     2641 acctcctcga aatacatgaa ataattctcg tccaaaaggc cgatggcctc gaagaccgcg
     2701 cggcgtacca tgaggctagc accagagctc cagtcgcagc ggctcgcaac gagcggtgca
     2761 ggggacgaga tgacccaccg cgacaagatt cgggtcacca ggccgagccg aaccccgcct
     2821 tccaactcgc ccaggacggt aggaaagcga aacgccgacc actccgcggt tccctctcgg
     2881 ttcacgagcc ggctgccggc gattccgaca gcgggatgat gctcgagaaa atcaaccagc
     2941 gc
//
LOCUS       DATAQL010000127         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_623905, whole genome shotgun sequence.
ACCESSION   DATAQL010000127 DATAQL010000000
VERSION     DATAQL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623905"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2965)
                     /locus_tag="VHS97_02120"
     CDS             complement(<1..2965)
                     /locus_tag="VHS97_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447017.1"
                     /translation="MDLFTFLIFGLSQLLLAQASVSHAPAESKPAAVSTPAGERLGAS
                     DRAFPWYVILSSPPDIAALWQSIEHPDLVVIKENQLVNKQAGDALSGADRAMAPRWVV
                     ESVAVRGAFGEDFADLKVDLLIDVKGAEPLWVPIRLDGRTLTRARQAGLELALRRSDG
                     GQWEVKLEGEGEHRILVELRAPLGAAAARKSLSLNIPEVASTAVELEFSHGESDIVIG
                     ANEVSGPHDGASGNEAHLTAHLSPRSKLDVSWTGNADAGVSNPPLLTAQGEIAIDIDD
                     EQVRTRSSWAIRCLRGTKHSLELQIDDDDEVTELTIDDQSAESRVERVRGTGKLMVRL
                     GDPLRTGSITQLVMKTRRPLMSSGARRVSFAGFPFANAREQSGFIGITQSANLWVKAA
                     TSQGLRRVDIGKLPADLRTRPSTSLAFEFLDQPFRLDLGVEPSPPLVRADSKTIFWIA
                     PERARSETTIELGWVRGRLSEVELSVAPGLELTSVGPADVVESSHLSDLIAARDPSGA
                     TSRERRLRIRLTSLGRDANKVTLKLTGLQRIPPEGKVGLGLFTPIQAASAHAAFALIA
                     DRGLALALADDSGRIRISSESPLTANKLAAAGTWSTLREELEGSAMLLVDDDNSRLLE
                     INITHHARTLVQETVLAVQVSKRWVDVLERSTFGVHHGVLSSLEMLVPAEIVDRWELL
                     EKELSDREELGREPGGARRYRLTFVRPVVDKLTLRFRYRLNLASALNATTAQEITIPS
                     ISFKEVPPGPTNVEMSLAPEIVLKETLKGWIRSSEDARSEPSAEGAILSFVESDPGAR
                     GRQFQFKALALEPVPLPSLVASRLLLKTVSSDDDSARTSASYWVESHGPDFAFALPDA
                     ARWIGARVDGRIAEHVDYNPADGGYRLRFPGEVGSKSALVELQYEHGNKGDANTWQAP
                     RLLDGGVALQTLWELRLPSSLALVGVPRGWSDQNQWAWSGITWKRCPWQDMTAVNEWI
                     GGAGAVSRTGEEFDVA"
     gene            complement(3267..5249)
                     /locus_tag="VHS97_02125"
     CDS             complement(3267..5249)
                     /locus_tag="VHS97_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447018.1"
                     /translation="MRLTLRTLLAWLDDTLQPTQVREIGGQVAESPFAQELSERIHRV
                     TRQRRLSVPSSSGPDGTDPNVVASYLDNDLDPEAVAEYEKKCLTSDVKLAEVASVHQI
                     LSLLGQRVKVPAEARTRMYQLVKGREALRPRARDAKRAEVPEPLTKPIQPWVVPDTPR
                     RNLFERIGPVAAGLALIGLSVLTALWSLRAPQTEIVYPSGPPRDRVPGDAGANASAKD
                     NAASPMPDGPQELETGLPEDARVASNEKSAAAGPDSAVAPKPTDAAESKATKAADPSV
                     AAKDSAVPKPVPADSSGIAEADGVVLLRYNNDQREWDRLVGSTSVNKSDRILCLSPFR
                     ATITLGKIPIFLAGETEVRILSRSSDKIPAIELIQGRLLLRNPPAASLKVGFSDRNIT
                     LELTPSSSVVLERTDRREYGQTVTQAPPLVVYCTAGEVPLTADLKQETLTASDVVVID
                     TAGGVKRTAIDTPPAWATDAEPSPREVQIRDQFLRMFHPGRPVLTEIVAASEDDNADN
                     KRLSILALKSLGDLSLLMPMLSRKDDPVTRTTALNAIRTFMGLGRESANRVREQLSEE
                     FGDDTALFVEKMLIGITPDEASNPQIYERLVSLLGPERESVGIRELAIDTLKRLTGRD
                     DLGYDPDHPEAKGLNAWKDLQKQGKLRYSATRGKAK"
BASE COUNT         1032 a         1662 c         1570 g         1053 t
ORIGIN      
        1 tggcgacgtc gaattcttct cctgtgcgag agaccgcacc ggcgccgcca atccattcat
       61 tcactgccgt catgtcttgc catgggcagc gcttccacgt aatgccactc caagcccatt
      121 gattttggtc ggaccatccc cgcgggactc cgactaacgc caggctcgat ggaagccgta
      181 actcccagag agtctgtaga gctacgccgc catccaaaag ccgcggagcc tgccaggtgt
      241 tcgcgtcgcc cttgttaccg tgttcatact ggagttcgac cagcgccgac ttggatccca
      301 cttcgcccgg aaaccgcaga cggtagccac cgtcggcggg attgtagtcg acatgttcgg
      361 caattcggcc gtcgaccctg gccccgatcc agcgagcggc gtcggggagt gcgaaggcaa
      421 agtccggccc gtgcgattcg acccagtacg aggcgctggt gcgcgccgag tcgtcatcgc
      481 tcgagaccgt tttgagcaag agccgtgacg ccaccagtga cggcagcggc accggctcaa
      541 gtgcgagcgc cttgaactga aactgacgcc cgcgcgcacc cggatcactt tcgacgaacg
      601 acaagatcgc cccctcggcg ctcggctcgg agcgcgcgtc ttcggaagag cggatccagc
      661 ctttcaatgt ctctttcaga acgatctcag gcgcgagcga catctccacg ttcgttggcc
      721 ccggcggaac ctccttgaat gaaatcgagg ggatcgtgat ttcctgtgcc gttgtggcgt
      781 tcagggcaga agcgaggttt aggcgatatc gaaaacggag cgtcagcttg tcgacaaccg
      841 gccgcacaaa tgtcaaccgg taccgccgcg cgccgcccgg ctcccgaccc agctcttcgc
      901 ggtcggacag ttctttttcg agcagctccc agcgatcgac tatctcggcc ggcactaaca
      961 tctccagtga gctgagcacg ccatggtgca cgccgaaggt tgatcgctct agcacgtcga
     1021 cccacctttt cgaaacctgg accgcgagca cggtttcctg gaccaacgtt cgggcatggt
     1081 gcgtaatatt gatttcgagc aaccgagaat tgtcgtcatc cacgagcaac atcgcgctcc
     1141 cctccagctc ttcgcgcaac gttgaccacg tgccagccgc cgccagcttg ttggctgtca
     1201 ggggagattc gcttgatatt cgaatccgcc ccgaatcgtc tgcgagcgca agcgcgaggc
     1261 cgcggtctgc aatcagcgca aacgctgcgt gcgccgaggc tgcttggatg ggtgtgaata
     1321 atcccaaccc cactttgccc tctggcggta ttcgctgcag tccagtgagt ttgagcgtca
     1381 ctttgttcgc gtcgcgacct agcgatgtta aacgaattct cagacgccgc tcacggctcg
     1441 ttgccccgct tggatcgcgt gctgcgatca ggtcggaaag gtgcgagctc tcgacgacgt
     1501 cggccggacc gacagaggtc agctcgagtc caggcgcgac gcttagctcc acctcggaga
     1561 gtcggccgcg aacccagccg agctcgatcg tcgtttcact gcgggcgcgc tccggcgcga
     1621 tccagaagat ggtcttcgag tcggccctca ctaggggcgg agaaggctca accccaagat
     1681 ccagccgaaa cggttgatcg aggaactcaa aggccaggct cgttgagggt cgcgtgcgca
     1741 ggtcggcggg caacttcccg atgtcgaccc tgcgcagccc ttgagacgtc gccgccttga
     1801 cccagaggtt tgcactctga gtgatgccga tgaaacccga ttgctcccgc gcattggcaa
     1861 aaggaaagcc ggcaaatgaa actctgcggg cacccgagct catgagcgga cggcgggttt
     1921 tcatcaccaa ttgtgttatt gagcccgttc gcagaggatc acccagacga accatcagct
     1981 tcccggtccc gcgcacgcgc tcgacgcgtg actcggccga ttgatcgtcg atcgtgagct
     2041 ctgtgacctc gtcatcgtcg tctatctgca gctccagact gtgcttcgtg ccgcgcaggc
     2101 aacggatcgc ccatgaggac cgcgtccgca cctgctcgtc atcgatgtca attgcgattt
     2161 caccctgtgc cgtcaacaga ggaggattcg acacgccggc atccgcattg cccgtccagc
     2221 tcacgtcgag cttggaacgc ggtgatagat gagcagtcaa atgggcttcg ttgccgctgg
     2281 ccccgtcgtg cggcccggag acttcattag ctccgatgac gatgtctgac tcaccgtggg
     2341 aaaactcgag ttcgacggcc gttgaggcga cttccggaat attaagagac aacgacttgc
     2401 gagccgcggc tgcgccgagc ggagccctca attcgacaag aatgcgatgc tccccttcgc
     2461 cttccagctt gacttcccat tgcccgccat cgctccttct caaagccagt tcaaggcccg
     2521 cttgccgcgc gcgggtcaac gtcctgccgt ccagccggat cggtacccag aggggttcgg
     2581 ctcccttaac gtcgatcaac aggtcaacct tgaggtcagc aaaatcttcg ccgaaagcac
     2641 ctcgaaccgc gacagattcc accacccagc gcggggccat ggcgcgatcc gcgcctgata
     2701 aagcatcgcc ggcttgcttg ttcaccagtt gattctcttt gatcaccaca agatcaggat
     2761 gttcgatgct ttgccagagc gcagcgatat cgggcgggct gctcaggatg acataccaag
     2821 ggaacgcgcg atcgctggca ccgagccttt cgccagcagg agtcgacact gcggcgggtt
     2881 tactttcggc cggcgcgtgg gaaacgctcg cctgagccag cagcaattgg ctcaagccga
     2941 agatcagaaa ggtgaataaa tccaaggcgc agcaccttca ggagttgcac agcttggccg
     3001 gacctacggc ggcgctgaac gcgcctcgac gatcccgacc tacgacgctt gagacgaaaa
     3061 tgacgcctcc caacgcgcaa tatcggtcga tcttaactat accacagtcg aacggattcc
     3121 tccatgcgcc gatcggcccg agccggcaac catgcatgaa tccgctacgt aaatctcagc
     3181 ctggatcgcg gcgtttcgag tggcgacagg tgacgatcgg atgcgaatcg caagatgatg
     3241 gggaccaagt cgcggcgggc gatccgtcat ttcgccttgc cgcgagtagc cgagtaacga
     3301 agcttgccct gtttttgtag atctttccag gcgttcaagc cttttgcctc gggatggtcg
     3361 ggatcgtatc ccagatcatc ccggcccgtg agccgcttga gtgtgtcaat cgccagctcg
     3421 cggatcccca ccgattcccg ttcgggtccg agcagtgaga ccagacgctc atagatttgg
     3481 gggttcgacg cttcatcggg cgtaataccg atcagcattt tctcgacgaa tagggcggta
     3541 tcgtcgccga attcttccga gagttgctct cgaacgcggt tggccgactc gcggccgagt
     3601 cccatgaagg tgcgaatcgc gttcaaggcg gtggtccgcg tcacgggatc gtccttccgc
     3661 gacagcatcg gcatcaaaag cgacagatcg ccgagcgatt tcagggccag gatcgacaat
     3721 cgcttgttgt cggcgttgtc gtcttcgctt gccgcgacga tctcggtcag cacaggtcgg
     3781 ccgggatgga acatccgcag gaactgatct cgtatctgaa cctcgcgcgg ggacggctca
     3841 gcgtcggttg cccaagcggg gggtgtatca atcgcggttc gcttgacgcc gcccgcggtg
     3901 tcgatcacca cgacgtctga ggccgtcagc gtttcctgct tgaggtcggc ggtcagcggc
     3961 acttcgccgg cggtgcaata gacgacgagc ggtggggcct gagtaacggt ttgaccgtac
     4021 tcccggcgat ccgttctctc caggacgaca ctgctcgaag gcgtaagttc cagggtgatg
     4081 ttcctgtcgg agaaacccac cttgagcgat gcggcgggag gattgcgcag caagagccgg
     4141 ccctgaatca gctcgatcgc ggggatctta tccgatgaac gagagagaat ccggacctcg
     4201 gtctcacccg caagaaagat aggaatcttc cccagagtga tcgtcgcccg aaacggcgag
     4261 aggcagagga tccggtcaga tttgtttact gatgtcgagc caaccaggcg atcccactcc
     4321 cgctgatcgt tgttatagcg caagagtacg actccatcgg cttcagcaat accggacgag
     4381 tcggcaggca caggcttggg gaccgcggaa tccttggcgg ccaccgaagg gtccgctgcc
     4441 ttggtagcct tcgattcggc cgcatcggtt ggtttgggtg cgacggccga gtctggacca
     4501 gcggcggccg atttttcgtt gctcgcaact cgagcgtctt ccgggagtcc ggtttcgagc
     4561 tcttgtggcc catccggcat cgggctcgcc gcattatcct tggccgatgc atttgcgcca
     4621 gcatcgccag gcaccctatc cctcggagga ccagacggat aaacaatctc cgtctgaggg
     4681 gctcgtagac tccacaacgc cgtcaggacc gacaatccaa tcagagctaa accggccgca
     4741 accgggccga tccgctcgaa caaattgcgt cgcggcgtat ccggcaccac ccacggctgg
     4801 atcggcttgg tcaacggctc gggaacctca gctctcttgg catcgcgcgc cctcggccga
     4861 agggcctctc gccccttcac aagttgatac atgcgcgtcc gcgcctcggc gggcaccttc
     4921 accctctgac ccagtaaact gagaatctga tgaacgctcg ccacttctgc gagctttacg
     4981 tcggaggtga ggcacttctt ttcatactcc gcgaccgcct ccggatcgag gtcattatcg
     5041 aggtagcttg caaccacgtt ggggtcggtt ccgtcggggc cggaactgct tggcacggaa
     5101 aggcgacgct gccgcgtgac gcggtgaatt cgttccgaaa gttcctgagc gaacggactc
     5161 tcggcgacct ggccgccgat ctctctgacc tgtgtcggct gcagcgtgtc gtcgagccaa
     5221 gccaaaagcg tcctaagagt taaacgcatg ccgaaaacct cgatcgtgtc ggttggatgt
     5281 tttgattaac ggattgagca aggatccgcg gacgcgc
//
LOCUS       DATAQL010000128         4777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5357108, whole genome shotgun sequence.
ACCESSION   DATAQL010000128 DATAQL010000000
VERSION     DATAQL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5357108"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2874)
                     /locus_tag="VHS97_02130"
     CDS             complement(<1..2874)
                     /locus_tag="VHS97_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3447019.1"
                     /translation="MSDSRNIRSIRSDTIPEGSTDTPDDSSAPTSGSIAEAAKEGVDS
                     ISEPRAPLVPLAAAPTPDPSAPIAHSGVDETALFDGADQPRELPHTDNESFGLAQYHN
                     RILVRTWSHGRVEAAEHARLARPVVLRVLDAEAARDLAVRHEFLRQAREVALLDHPNL
                     GTGVLEAISDGPELYVAFSLSADRPLEGIGPRRAWLRSRDCLPYDNRRLALLLTDGAR
                     GLQALHQVGLVHGNLRRENLRAVPHTDRLRLHGLSSTPLVIDRREHKPPADDLADLAA
                     AIAVLATGQEAPLRAARAKPIARALRRANPGLDPELAEILGRCVATELPDRLDRADEL
                     ITRLEPLTRHRVVLATWGDRLVTLVYDCVLALIVYMLIRLLGLANSQNALLRAPALIP
                     ILWFPVILPLFEICVGWSPGRRAWRLRLVDVSGTRAARWKIGLRALLRIGWIGLAIAA
                     GYGLMWLVERASPIGLLWLSDDVTAIALGGVILLVTSRFTRARRPFYDVITGITWGIR
                     ETVAPPFVTGQSTPGGPSSARRQAVTELAVAGERVGPNRLEREIGRGGMGAVYAAWDE
                     ILDRPVAVKLITATCDASAEILKRFEQEARLAAQVRHENVAQVFSVGQDRHRPYMVLE
                     LLHGRTLQQLVEESGPVPLQAAWSFITQGATGLRAANQLGIVHRDIKPSNLMLTHEVV
                     VKVLDFGISKLVVEEEQGSETARAPKFDRMWSDPAWRDARLTRTGAVLGTPLYMSPEQ
                     AKGQPMDCRSDIYSLGMTLYYLLAGQPPFDCSDPLDLLAKQCEAAPPSLKDEVVGLTP
                     ERAEVLERMIAKDPEARFQNYEELLEALHATARLPERASLGPRVLAALLDLVLFSVIG
                     TLIAWIPVPGSLGRVSGPIGFHLAWILPAVGVWRWRVTPATWVIGLRVTRPHGGRVGL
                     VRSLVRQIAYEPVLILAPLASFLAWPTGTSLAIALNGMLWVVSL"
     gene            complement(2899..3228)
                     /locus_tag="VHS97_02135"
     CDS             complement(2899..3228)
                     /locus_tag="VHS97_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447020.1"
                     /translation="MQHRDWNDLENAIYSSMARLPIKAGSCLQRAPLVWTKLAWGGFL
                     RAALAVMNPHVLPALAERLRIAAPIVILELSAELILERDPRFLREMGVLGPEASARIF
                     ADRGVTL"
     gene            3611..>4777
                     /locus_tag="VHS97_02140"
     CDS             3611..>4777
                     /locus_tag="VHS97_02140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3447021.1"
                     /translation="MNEESLFVAALEKPTAAERHAFLEFACAGDSGLRQRVELLLAAH
                     DKTRGMLDASARPTGWTEVAAELTPGRGPHASELVGTKIADRYRLLEEIGAGGMGTVW
                     KAEQKQPVRRTVALKLIKAGMDSRTVLSRFEVERQALAVMDHPNIARVLDGGTTESGR
                     PYFVMEYVKGVPLTRYCDDARLSIAQRLALFVPVCQAVQHAHTKGVIHRDLKPSNILV
                     CLQDGQPMPKVIDFGLAKAIHQPLTEHTLHTAHGQLVGTPLYMSPEQAEPNNLDVDAR
                     TDVYALGVILYELLTGSTPVEGGRFHHATWHEMLRMIKEEEPQRPSARLSGSDSLPSL
                     AVQRQLEPVRLTRLLRGELDWIVMKALQKDRSRRYETANSFARDVERYLHDEPVE"
BASE COUNT          927 a         1511 c         1526 g          813 t
ORIGIN      
        1 caaagaaacg acccagagca tgccattgag tgcgatggcc aaagatgtcc cagttggcca
       61 cgcaagaaag gaggcgagtg gggccagtat caggactggc tcgtatgcga tctggcgtac
      121 cagtgagcgg accaggccga cgcgaccgcc atgtggtcgg gtcacacgga ggcctatcac
      181 ccatgttgcg ggcgtcactc gccatcgcca cacgcccacg gcgggaagga tccaggcgag
      241 gtggaagccg atcggccccg agacacgacc aagcgagccg ggaacgggga tccaggcaat
      301 cagcgttccg atcacggaga acagtacgag gtccaggagc gcggccaaaa cgcgcggccc
      361 gagcgaggcg cgctcgggga gccgcgcggt cgcgtgcagg gcttcgagca gttcctcgta
      421 gttttgaaat cgcgcctctg gatccttcgc aatcattcgc tccaggacct cggcccgctc
      481 gggggtgagg ccgacgacct catccttcag gctcggaggc gccgcctcgc actgcttggc
      541 gagcaggtcg agggggtcac tgcaatcgaa cggcggctgg ccggccagga gataatagag
      601 ggtcattccc agtgagtaga tgtcgctgcg gcaatccatc ggctggccct tggcctgctc
      661 gggcgacatg tacagcggcg ttccgagcac agcaccggtc cgcgtcaagc gcgcgtcgcg
      721 ccaggcgggg tcggaccaca tccgatcgaa cttgggcgcg cgggcggtct cgctcccctg
      781 ctcttcctca acgaccagct tcgagatacc gaagtccaga accttgacaa cgacttcgtg
      841 ggtgagcatc aggttcgatg gtttgatatc gcgatggacg atccccagct ggttggctgc
      901 ccgcaatccg gtggcgccct gcgtgatgaa ggaccaggcc gcttgcaacg gcacgggccc
      961 gctctcctcg accaactgct gtagtgtccg cccgtggagc aattcgagca ccatgtacgg
     1021 cctgtgccgg tcctgtccga cactgaagac ctgcgcgaca ttttcatgcc gtacctgcgc
     1081 ggccaggcga gcctcttgct cgaagcgctt gaggatttca gctgacgcat cacacgtcgc
     1141 ggtgatcagc ttgaccgcga ccggtcggtc caggatctca tcccaggcag catagacagc
     1201 acccattccg ccccggccga tctctcgttc gagacgattc ggtccaactc gctctccggc
     1261 aacggccagt tcagtcaccg cctgtcgccg cgctgagcta gggccgccgg gcgtcgactg
     1321 gccagtaacg aaaggagggg ctaccgtttc gcggatgccc caggtaatgc cagtgatcac
     1381 gtcatagaac ggccgacgag cgcgggtgaa ccggctagtc accaggagta tcacgcctcc
     1441 aagcgctatc gcggtcacgt cgtcgctcag ccagagcaat ccaatcggag aggctcgctc
     1501 caccagccac atgagcccgt aaccggccgc gatggccaaa ccgatccagc cgatgcgcag
     1561 cagcgcgcgc agcccgattt tccatcgggc tgcccgggtc ccggagacat ccaccagccg
     1621 aagccgccat gcccgtcgcc cgggtgacca gcccacgcaa atctcaaaca gtggcagaat
     1681 cacgggaaac cagaggattg gtatcaaagc gggcgcccgc aaaagggcgt tctgcgaatt
     1741 cgccaggccc aacagccgta tgagcatata cacaatcagg gcgagaacgc agtcgtatac
     1801 caaagtgacc agccggtctc cccacgttgc cagaacaacg cggtggcgag tcagcggctc
     1861 cagacgtgtg atcagctcgt ccgcgcggtc gagccggtcg ggtagctctg tggcaacgca
     1921 acggccaaga atctcggcca gctcggggtc gaggccaggg ttggctcggc gcagcgcccg
     1981 cgcgatcggc ttcgcccgag cagctcgcaa tggggcttct tgcccggtcg cgaggactgc
     2041 aatggccgct gccaggtcgg cgagatcgtc cgccggcggc ttgtgttccc gcctatcgat
     2101 tacaagcggc gtcgatgata ggccatggag tcggagccgg tcggtgtgag gcacagcccg
     2161 caagttttcg cgtctgaggt tgccgtgcac caaccccacc tggtgcaggg cctggagccc
     2221 gcgcgccccg tcggtgagca gcagggccag gcgtcgatta tcgtagggca ggcagtcgcg
     2281 acttcgaagc caggcccgcc gcggtccgat tccttccagc ggccgatccg ccgaaaggct
     2341 aaaggcgacg taaagctctg gaccatcgct gatcgcttcg aggacgccgg tgccaaggtt
     2401 agggtggtcg agcaatgcga cttctcgcgc ctggcgaaga aactcgtggc gcacagccag
     2461 gtcgcgcgcg gcctccgcat cgagcactcg taacacgacc ggtcgagcca atcgagcgtg
     2521 ctcggccgcc tcgacccggc catggctcca ggttcggacc agaatccgat tgtggtactg
     2581 cgccaagccg aaggattcgt tgtctgtgtg cggcaactct cgaggctgat ccgcgccgtc
     2641 gaacagggcg gtctcatcga ctccggaatg cgcgatcggt gctgacgggt ctggagtcgg
     2701 cgcggcggcg agtggaacca atggcgctcg cggctcggag atcgagtcaa caccctcctt
     2761 cgccgcctcg gcgatcgagc ccgatgtcgg cgccgagctg tcgtcaggtg tgtccgtgga
     2821 gccttctggg atcgtgtccg accgaatcga ccgaatgttg cgagaatcgc tcatggtttc
     2881 gtaccgacga ttggacgctc agagcgtgac gccacggtcg gcgaagatcc tcgcagacgc
     2941 ttccgggccg agcacgccca tctccctcaa gaagcgaggg tcgcgttcca gaatcagctc
     3001 agcagacagc tcaagaatga cgatgggggc agcaatccgc aagcgttccg caagagctgg
     3061 caagacatga ggattcatga cagcaagcgc cgcgcggaga aagcctcccc aggcgagttt
     3121 tgtccacaca agcggcgcgc gctggaggca tgatcccgct ttgatcggca accgggccat
     3181 gctagagtaa attgcgttct caagatcatt ccagtcacga tgttgcacga tatcgtttca
     3241 tgttcgatcc atattctcct acttcatgtc ggtatttcgg ccctgattgc gagacattat
     3301 tcgactggtt cgaatttcac ccgcagattg gccggctgga ctggtccagg ctgctttatg
     3361 aggggtcgct gttgacatca gggaaccctg ttccagaaac acacgtgcgc cgcgccgatt
     3421 ttccagctcc gcgagcatcg ggaatttcac caagacgcac gctcggtcga cctggaagtc
     3481 ggcagagctc tgattcgccg attcagtgac tcggtttaat ttcgccgcga gatcgatcgg
     3541 gttgtggtac tggcggtgtc tccgaagtct cctgaccaac ggccccttgc gggccgggag
     3601 cgctcgagcg ttgaacgaag aatcgctttt tgtggctgcc ctggagaagc cgaccgcggc
     3661 cgagcggcat gcctttctgg aattcgcgtg tgccggcgac agcgggctgc gacagcgagt
     3721 ggagctgttg ctggcggccc acgacaaaac tcgtggcatg ctggacgcgt cggccaggcc
     3781 gaccggatgg acggaagtcg ccgccgaatt aacccccggc cgtgggcctc acgcgtccga
     3841 actcgtcggt accaagatcg ccgaccgcta caggctgctc gaggaaatcg gcgcgggggg
     3901 catgggcacg gtctggaagg ccgagcaaaa gcaaccggtc cgccgaacgg tggcgttgaa
     3961 gctgatcaag gccggcatgg attcgagaac cgtcctgtcg cggttcgaag tggaacggca
     4021 ggccctggct gtgatggacc acccgaatat cgccagggtg ctggacggcg gcacgaccga
     4081 gagcggtcgg ccgtatttcg tcatggaata cgtgaagggc gtccccctga cccggtattg
     4141 cgacgacgcg cgcctgagca ttgcccagcg actcgcgctc ttcgtgcctg tgtgccaggc
     4201 cgtccagcac gcccacacca agggtgtgat ccacagagac ttgaagccca gcaacatcct
     4261 ggtctgcctt caagacggcc agccgatgcc caaagtgatc gacttcggtc tggccaaggc
     4321 gatccaccag cccctcaccg aacatacgct gcacaccgcc cacggccagc tcgtgggcac
     4381 gccgctttat atgagtccgg aacaggccga gcccaacaac ctcgatgtgg acgcccgcac
     4441 cgacgtctac gcccttggcg tgatcctcta cgagctcttg accgggtcga cgccggtgga
     4501 gggaggacgt ttccaccacg cgacctggca cgaaatgctc cgcatgatca aggaggaaga
     4561 gccgcagcgg ccgagcgccc ggctcagcgg cagcgactcg ctgccgagcc tggcggtaca
     4621 gcgacaactt gagccagtcc gtctgaccag gctgttacgg ggcgagctag actggatcgt
     4681 gatgaaggcg ctgcaaaagg accgctcgag gcgctatgag acggccaatt cgttcgcccg
     4741 ggatgtcgaa cggtacctgc acgatgagcc ggtagag
//
LOCUS       DATAQL010000129         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3067245, whole genome shotgun sequence.
ACCESSION   DATAQL010000129 DATAQL010000000
VERSION     DATAQL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067245"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VHS97_02145"
     CDS             <1..456
                     /locus_tag="VHS97_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3447022.1"
                     /translation="GTGKEQTIKIESSGGLTKEEIDRMQRDAQAHAAEDKRRRELAEA
                     RNAAEQRVYQLEKLLDENKNRLSEADTAAVRSAIVQVNEAKKGNDPSAIHRAIDDLQQ
                     ASQAMIQHLQATSSPNVDGPGSGGHGPARERGPYGGQAEEVIDAEFEKK"
     gene            complement(660..1877)
                     /locus_tag="VHS97_02150"
     CDS             complement(660..1877)
                     /locus_tag="VHS97_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX3447023.1"
                     /translation="MREGPVVEWIRSKYQGLSAEFTERARRRWAAVEASSLGWGGISA
                     VSHATGIARTTIARGIRELESTGVKSTGRQRRAGAGRKRADEVDPELVAALDRIVEPE
                     SRGDPMTPLRWTCKSTRNLAKELTAQGHSVSASTVRQLLRNQGYSLQANRKTREGASH
                     PDRDAQFRRIGRRVTSQLRAGQPAVSVDAKKKETIGKLKNPGRTWRRKREPLKVDVHD
                     FPDPKKGKAVPYGVYDLKHNEAWVSVGISSDTAEFATESIRRWWRRMGRPRYPGAKRL
                     LITADSGGSNSSRCRLWKVELQKLADELNLEIEVCHFPPGTSKWNKIEHRLFCHVTRN
                     WRGRPLESFEIVVNLIGSTTTATGLQVHAQIDGSGYTKGRKVTNAELARVNLKPARFH
                     GEWNYVIGPQLQP"
     gene            complement(2052..>2480)
                     /locus_tag="VHS97_02155"
     CDS             complement(2052..>2480)
                     /locus_tag="VHS97_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447024.1"
                     /translation="KKLQNDTTALLQNAQQRFPTLRIAYLSGRIYGGWAVTSLNPEPY
                     AHEGNLVVRWMIRDQMSGSPELNHDPAKGPVKAPLLLWGSYLWADGTIPRKSDGLVWE
                     RQDLAADGTHPSQSGRRKVAEMLLKFFESDPLARTWFVKH"
BASE COUNT          473 a          788 c          722 g          497 t
ORIGIN      
        1 ggtaccggca aggagcagac aatcaagatc gagtcctccg gcgggctgac caaggaggag
       61 atcgatcgga tgcaacgcga cgcccaggcg cacgccgcag aagataagcg ccgccgtgaa
      121 ctcgccgagg cccgtaacgc cgccgaacag cgcgtctacc agctcgagaa gctgcttgac
      181 gagaacaaga atcgactttc cgaggctgac acggcggccg tccgcagtgc catcgtccag
      241 gtcaacgagg ccaaaaaagg gaacgaccca tccgcgattc accgcgcgat cgatgacctt
      301 cagcaagcca gccaggcgat gatccagcac ctccaggcca catcgtcgcc aaacgtcgac
      361 ggcccgggtt ctggcggaca cggccctgcg cgcgaacggg gcccgtacgg tggccaggct
      421 gaggaggtca ttgacgccga attcgagaag aaatagagat atgcggcagc gttcggacag
      481 gttgttactc gggagccgac tccgaggcat gccaggcgac aacctgccac ttgaagtcgg
      541 aaccacttgt ggtcgctgga cgaggttcgg gaagccaggc gcgtacgccc acattgctcg
      601 ccacgcagtg aagtgcccgg agtgacgtta ggcgccgcgc gaaaataagt gctgcaagtc
      661 tacggttgca gttgtgggcc gatgacgtag ttccattcgc cgtggaaccg tgccggtttc
      721 aggttcactc gggccaattc cgcgttcgtc accttgcgac ctttcgtgta gccactgcca
      781 tcaatttgag cgtggacttg tagtcccgtc gcggtcgtcg tggagcctat caggtttacc
      841 acgatttcga agctctccag cggacgcccg cgccagttcc gggtgacgtg gcagaagagc
      901 cggtgctcga tcttattcca cttgctcgtc ccagggggaa aatggcaaac ctcgatctcc
      961 aagttcagtt cgtcggcgag tttttgtagc tcgaccttcc acagccgaca tcgactgctg
     1021 ttgcttccgc cgctgtcggc tgtgatcaaa agccgtttcg caccaggata acgcgggcgt
     1081 cccattcggc gccaccagcg gcggatcgat tccgtcgcaa actcggccgt gtcgctgctg
     1141 atcccgacgc taacccaagc ctcgttatgc ttgagatcgt agacgccata tggcacggct
     1201 ttgcccttct tcgggtctgg aaagtcatgc acgtcaacct tgagaggctc gcgcttccgg
     1261 cgccacgtcc gaccaggatt cttcagtttc ccaatcgtct ccttcttttt cgcatcaacg
     1321 gaaaccgccg gttgaccggc ccgcaactgt gaggtcacac ggcgaccgat tcgtcggaat
     1381 tgcgcatccc gatcggggtg gctagctccc tcgcgtgttt tgcgattggc ctggaggctg
     1441 tagccttgat tccgcagcag ctgtcgtacg gtgctcgcgc tcactgaatg cccttgcgcc
     1501 gtcaactctt tagcaaggtt tcgcgtactc ttgcaagtcc atcgcaaggg cgtcattgga
     1561 tcgccgcggc tctcaggctc gacgattcgg tccaacgcgg cgaccaattc cggatcaact
     1621 tcgtcggctc tcttacgtcc agctcccgcg cgacgttgcc tcccggtcga cttaacacca
     1681 gttgactcga gctcgcggat gccacgggcg attgtggtgc gagcgatccc cgtagcgtgg
     1741 ctcactgccg aaatgccacc ccaacccaac gaactggctt cgacggcagc ccatcggcgg
     1801 cgagcgcgct cagtaaattc cgcggagagg ccctgatact tcgagcgaat ccattcgaca
     1861 accggtcctt ccctcatggg aatcgtcatc gacgaaaggc gccagttcgt caatggcaag
     1921 ttgcagcatt aattttcgcg cggcgcctta gcatgttcct caccctccgg cacaccgaaa
     1981 accggatctg accccttgga ccccggatct gaccccttgg acaatcactt gttctgcagg
     2041 tcaattcagc ctcaatgctt cacgaaccac gttcgggcca gcggatcact ctcgaagaac
     2101 ttcaggagca tctccgccac cttcctgcga ccgctctggc tcggatgcgt gccgtctgcg
     2161 gccaggtctt gccgctccca gaccaggccg tcactcttgc gagggatcgt accgtcggcc
     2221 cagaggtacg agccccagag cagtaatggc gccttgacgg gccccttcgc cgggtcgtga
     2281 ttcagctcgg ggctcccgct catctggtcg cgaatcatcc agcggacgac cagattgccc
     2341 tcatgcgcgt aaggctcggg attcagcgaa gtgacggccc agccgccgta gatccggccg
     2401 gagaggtagg cgattcgcag ggtggggaag cgctgttgcg cgttttgcag aagcgccgtc
     2461 gtgtcgtttt ggagcttctt
//
LOCUS       DATAQL010000130         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3678226, whole genome shotgun sequence.
ACCESSION   DATAQL010000130 DATAQL010000000
VERSION     DATAQL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3678226"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /gene="proA"
                     /locus_tag="VHS97_02160"
     CDS             complement(<1..526)
                     /gene="proA"
                     /locus_tag="VHS97_02160"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566405.1"
                     /note="Catalyzes the phosphorylation of L-glutamate during
                     the proline biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX3447025.1"
                     /translation="MSAVAEIDERDDPSTVVTLSLCRTLAQEAKLASRHLAWARGSAK
                     NAWLIGSAVALLERREEILAANGRDVAAAPEQGLNAAAVDRLTLNARRIEEMAQTLRD
                     VAAAPDPVGEVVASSRRPNGLEVKQVRVPLGVIFMIYESRPNVTVDAAALCVKSGNSV
                     ILRGGKEAIHTNRAL"
     gene            complement(523..1392)
                     /gene="folP"
                     /locus_tag="VHS97_02165"
     CDS             complement(523..1392)
                     /gene="folP"
                     /locus_tag="VHS97_02165"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX3447026.1"
                     /translation="MNTTSLVRWEARGREILPALDRVPKVMGIVNLTPDSFSDGGLSM
                     NHEAAVSHARRLAAEGADILDLGGESSRPGAEPVALEEELRRVIPVVETLAAEMSIPI
                     SVDTNKAEVARQALKAGASIINDISALGFDPAMAAVAAESGVGVVLMHMRGTPATMQM
                     SMQYNDVVAEVYDFLARRIEWTLAQGIRRERIAIDPGIGFAKTHKHSFEILRSLRRFE
                     SLECAILIGTSRKGLLGKITGRSVVERAAGSVASSLAACLNGARVVRVHDVAPMVDTI
                     KVWTVLQGWGDSP"
     gene            complement(1478..2035)
                     /gene="lptE"
                     /locus_tag="VHS97_02170"
     CDS             complement(1478..2035)
                     /gene="lptE"
                     /locus_tag="VHS97_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX3447027.1"
                     /translation="MFMVGSILAGLGGCGSFGRAPSARSSDGGFFGWHFNAPFDTSEV
                     KTVSVYFKTQSFRRDVEKQLTEAVEKEISMRTPYRVVGNHEKADSVLTGLITYADKNL
                     VVEAPTNFARELNMTISVSVKWTHNPPNEVEDARPPTIVTELNNFVPEVGETSLSATN
                     HVVQNLAKQIVDMMEQPWFNNSDLD"
BASE COUNT          415 a          708 c          629 g          434 t
ORIGIN      
        1 ggagcgcccg gttggtgtga atcgcctcct ttccgccgcg caagatcacg gagttcccgc
       61 ttttgacgca taatgcggcg gcgtcgaccg tgacatttgg acggctctcg tagatcatga
      121 agatgacgcc gagcggaacg cgcacctgct tgacctcgag gccatttggc cggcggctcg
      181 acgccacgac ttcgccaacg gggtcgggtg cggcggcgac atctcggagc gtctgcgcca
      241 tctcttcgat acggcgcgca ttgagcgtca gccggtccac ggcggcagca ttcaatccct
      301 gttcaggcgc ggccgcaacg tcgcggccgt tggccgccag gatctcttcg cggcgctcga
      361 gcaacgcgac ggccgagccg attaaccagg cgttttttgc tgatccgcgc gcccaagcga
      421 gatgccggga cgccagcttg gcttcttgag ccaacgtgcg gcagaggctc agcgtgacca
      481 cggtgctcgg atcgtcgcgc tcgtcaatct cagcgaccgc gctcatggcg aatcccccca
      541 accctgcaaa acggtccata ccttgatggt atcgaccatc ggagcgacat catgcacccg
      601 cacgacacgc gctccgttca aacaagccgc cagcgacgac gcgactgacc ccgcagcgcg
      661 ctcgaccacc gatcgccccg tgatcttgcc taaaagaccc ttgcgggaag tgccaatcaa
      721 aatcgcacat tccagactct caaatcggcg caagctccgc aagatctcaa aactatgttt
      781 gtgggtcttg gcgaagccaa taccgggatc gatggcaatc cgctcgcggc ggatcccttg
      841 ggccaaagtc cactcaatac ggcgtgccag aaagtcgtag acttcagcca cgacatcgtt
      901 gtactgcatg ctcatttgca tcgtggccgg ggtgccgcgc atgtgcatga gcacgactcc
      961 aacccctgac tcggcggcaa cagccgccat cgccggatcg aatcccaagg cggagatatc
     1021 gttgatgatc gaggcaccgg ctttcagggc ttgtcgcgcg acctcggctt tgttcgtatc
     1081 gaccgagatg ggaatcgaca tctcagcggc aagagtctcg acaaccggga tcacccggcg
     1141 cagctcttcc tcgagtgcga ccggctcggc ccccgggcgg cttgattcgc cccccaagtc
     1201 gaggatatcg gcgccttcgg ctgcgagccg gcgggcatga gacacggccg cctcgtggtt
     1261 catgctcagg ccgccgtcgg aaaagctgtc cggcgtgagg ttgacgatcc ccatgacctt
     1321 gggaacccga tcaagagccg gcaagatctc ccgcccgcgt gcctcccacc ttaccaacga
     1381 tgttgtgttc acgatttcgt tccggcctag ggccggctgg cacgcccgcc cttactcatg
     1441 cggcgcttcc aggcgacggc tcgccaaggc atctggctca atcgaggtcg gaattgttaa
     1501 accacggttg ttccatcata tccacgatct gcttcgccaa gttctggacg acgtggtttg
     1561 tggcgcttaa cgacgtttcg cctacttctg gcacaaagtt gttaagttcg gtgacgatcg
     1621 tgggcggtcg cgcgtcctcc acctcgttgg gcgggttgtg ggtccacttc acactgacgc
     1681 tgatcgtcat attgagctca cgtgcgaaat tcgtcggagc ctcgacaacc aggtttttgt
     1741 cggcgtaggt aatcagcccc gtcagcaccg agtcggcttt ttcgtgattg cctaccactc
     1801 ggtagggagt gcgcatgctg atctctttct cgacggcctc ggtcagttgc ttttccacgt
     1861 cgcgacggaa tgattgcgtc ttgaaataga cgctcacggt cttgacctcg gacgtatcga
     1921 aaggcgcgtt aaaatgccag ccaaagaatc ccccgtccga tgacctggcc gacggtgccc
     1981 gcccgaatga gccacacccg cccaggccgg ccagaatcga tcccaccatg aacatgagcc
     2041 ggcgtcgccc agtcgaaaaa tcagggcccg cgctgctttc gcggctgtca attcgtcgcg
     2101 agcatcggaa aggtacattc ttcatcgcac ccttgctccc gggagaactg gacgcaggtt
     2161 ccagaccggc aagttacgca agctcg
//
LOCUS       DATAQL010000131         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4746119, whole genome shotgun sequence.
ACCESSION   DATAQL010000131 DATAQL010000000
VERSION     DATAQL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4746119"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VHS97_02175"
     CDS             complement(<1..209)
                     /locus_tag="VHS97_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX3447028.1"
                     /translation="MLSSKLSVGVHILTLLALKPDEPVTSACIAGSVNTNPVVIRRLL
                     GLFREAGFVESKNGVGGGWVLVTDP"
     gene            complement(395..>2050)
                     /locus_tag="VHS97_02180"
     CDS             complement(395..>2050)
                     /locus_tag="VHS97_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3447029.1"
                     /translation="YDSVLEFTAHHWWTIVAPSVILLGLTGWLVAARPKSFIPTEDQG
                     YLIVTVQTPDGTSSEATQRVLTRVESLCRGTEGIRHTVALEGLNVITSANQTNCGVVF
                     LPLKEWSERSTPELRAVGLAALIQQKLSSEIRDASVMVLQPPPIRGLSSTGGFELMVE
                     DRSGKGTDELQRVIDRFQDEARKRPELAGIFTTFSARVPQLKFDIDRTKARRLDVPIS
                     DLFAVLQANLGGYYVNDFDLYGKVWKVMVQAEGGVRSKPEDIQNLYVLNRQGERVHLS
                     SLGEVRYALGPIDVPHYNLYGAAKINGGPALGFSSGQALAAMEEVAAKVLPEGFGYEW
                     TGTTLQEQKTGNQAAYIFALSVICVFLFMAALYESWIRPLVIILTVPLAMFGAVVGLW
                     LYDMPLDVFGQIGLVMLIGLETKNAILIVEFGVEMRQKRAMTILESAKAASRERLRPI
                     LMTSFAFVMGVLPMARATGAGAYSRNSLGIVIAFGIAVSTVLGRFVIPIYYVLGERLI
                     DFLSRYRGDGGEPDAPGVHSHLDGHRDKALIAEDGAFATMPLH"
BASE COUNT          418 a          624 c          625 g          383 t
ORIGIN      
        1 ttgggatcgg tcaccagcac ccagccgccc ccgacaccat ttttggactc cacgaagccg
       61 gcttcgcgga acaagccgag caatcggcga atcaccaccg gattggtgtt aacgctgccg
      121 gcaatgcacg ccgaggtcac gggttcatct ggcttgaggg cgagcaaggt caggatgtgg
      181 acgccaacac tcagcttgga acttaacatc gcgacccctc cgtccttgca gcgatactaa
      241 ctgcaaatgg gaacgctgtg aatacgagcg cactcggatt gaatatggac tttgacttgg
      301 tcgtggtacc tgaggttaca tcgccccccc caccctggcc ctcccccaga aggggggagg
      361 ggactataat gtgctgtgca catctaatgc gctgctaatg taagggcatc gtggcgaaag
      421 cgccgtcctc cgcgatgagg gccttgtcgc gatggccatc taaatgtgag tgaacgccgg
      481 gagcgtctgg ctcgcctcca tcgccgcgat atcgcgacaa gaaatcgatc agccgttctc
      541 ccaggacgta atagatcgga atgacaaacc ggcccagcac ggtgctcacg gcgatgccga
      601 acgcgatgac aatgcccagt gagttgcggc tgtatgcgcc ggcccccgtg gcccgcgcca
      661 tcggtaaaac tcccatcaca aacgcgaacg aggtcatcag aatcggccgc agccgttccc
      721 gggatgccgc tttggccgat tcgaggatgg tcatcgcgcg tttctggcgc atctcgacgc
      781 cgaactcgac gatcaggatc gcgttcttgg tttccaggcc gatcaacatg accaggccga
      841 tctggccgaa gacatcgagc ggcatgtcgt acaaccagag acccacgacc gcgccgaaca
      901 tggccagcgg cacggtgagg atgattacca gcgggcggat ccagctctcg tagagtgccg
      961 ccatgaacag gaagacgcag ataaccgaga gggcgaagat gtaagcggcc tggttgcccg
     1021 tcttttgctc ctggagtgtc gtgccggtcc actcatagcc aaagccctcg ggtaacactt
     1081 tcgccgcgac ctcttccatt gcggcgagcg cctggcctga gctaaagccc agggcggggc
     1141 cgccgttgat cttggccgct ccatacagat tgtaatgcgg gacgtcgatc ggtcccaagg
     1201 catagcggac ttcccccaga gagctcaagt ggacccgctc gccttgacga ttgaggacgt
     1261 aaagattctg gatgtcttcg ggcttggagc gcacgccgcc ttcggcctgg accatcacct
     1321 tccagacctt gccgtagaga tcgaagtcgt tgacataata accccccagg ttggcctgaa
     1381 gcaccgcgaa aagatcggag atcggcacgt ccagccgccg cgccttggtg cggtcgatgt
     1441 cgaacttgag ctgagggaca cgggccgaga aggttgtgaa tatgccagcc agttcggggc
     1501 gcttgcgggc ttcgtcttga aaccggtcga tgacgcgctg gagctcgtcg gttcctttgc
     1561 ccgatcggtc ttccaccatc agctcgaaac cgccggtcga gctcaggccg cggatcggcg
     1621 gaggctgaag gaccatcaca ctggcgtcgc gaatttcgct tgagagcttt tgctggatta
     1681 aggccgccag gccgacggcg cgcagctcag gcgtggatcg ctcagaccat tccttgaggg
     1741 gaaggaagac cacgccgcag tttgtctggt tggccgaggt gatcacgttg agcccctcga
     1801 gggcgaccgt gtgcctgatc ccctcggtac cccggcagag cgactcgacg cgcgtcaaga
     1861 cgcgctgtgt cgcctcactg ctggtgccgt cgggcgtctg gaccgtcaca atcaggtaac
     1921 cctggtcttc cgtcggtatg aatgacttgg gccgggccgc gaccaaccag ccggttagcc
     1981 ccagcaagat gaccgaaggt gcgacgatcg tccaccaatg atgggcagtg aactcgagca
     2041 ccgagtcgta
//
LOCUS       DATAQL010000132         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2304417, whole genome shotgun sequence.
ACCESSION   DATAQL010000132 DATAQL010000000
VERSION     DATAQL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304417"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VHS97_02185"
     CDS             <1..455
                     /locus_tag="VHS97_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447030.1"
                     /translation="QKEIAQIQAEMARKNALQKVGEQSKGVIAGRSVQPILNPMVVVP
                     GMAEVKASSEAFPAPLEPVDDLPPATVVTHVLIVEPGKMIVRGTTSDGGTVKRVSVNG
                     QDARPVRPNFAEWEVLLPVPNGVVEPIAFAEDTSGNIEKQPHHATNGR"
     gene            complement(1690..>2600)
                     /locus_tag="VHS97_02190"
     CDS             complement(1690..>2600)
                     /locus_tag="VHS97_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX3447031.1"
                     /translation="PLVSMIALGPAIVHRVDTPEVTLLHWNVQWGGGPFRSQQTWAAQ
                     RTEILRGNPDLIVLSELPPADWLARLTDDLGPAASSVYLENNPGSSFWYPMGVFSRWP
                     IRLERRVALPGGAGMSVTADVRGRRLRLLCVDGQSNPFRSRLPFLVAITDLCRAASDA
                     GRPFDVIVGDFNTPSRSLGFDSLMDQGYTLASRSSAGWRGTFPSWFPLYDIDHVWLGP
                     GLIARACRLYNGPATDHRGQVVRFGPLIVGEQATDVRIDEKASPGQSVPRRMSTLARH
                     TRPTGRGGARRIGASASSRILQGSAE"
BASE COUNT          519 a          807 c          813 g          461 t
ORIGIN      
        1 cgcagaaaga gatcgcccag atacaggccg agatggccag gaagaacgcc ttgcagaagg
       61 tcggggaaca gtcgaaggga gtaatcgcgg ggcggtcagt tcagcccatc ttgaacccga
      121 tggtggtcgt gccgggtatg gccgaagtca aggcatcaag cgaggcattt ccggctccgc
      181 tcgagccggt ggatgacctg ccgcccgcaa ccgtggtcac gcatgtgttg atcgttgagc
      241 cgggcaagat gatcgtgcgc ggcaccacat cggacggcgg taccgtcaag cgggtctcgg
      301 tgaacggcca agacgccagg ccggttcgac caaacttcgc ggagtgggaa gtgctattgc
      361 ccgtgcctaa cggcgttgtc gagccgatcg ctttcgccga ggacacgtcc ggcaatatcg
      421 agaagcaacc gcatcacgca acgaacggac gatagtgtgg tggtcaacga ctgagcgtag
      481 ccccataatc aaggcctgct tttcgccaat gagacaagcg ttgaaacgcg tcggcgaacg
      541 gtaccagagt gctcggcaag cgcaccagtg ctgcgagact ggcatgaggg gaaacgccaa
      601 ggagtcacca cgagctaatg aatttcgtga tcttgggtaa cggcccagaa gagctcgcct
      661 gggccacgtg gctggatggg caagttgatc atcgtttggc agcagccttt ccgggtttct
      721 atcactcgac cttgtcgggc attccgacag caagcgatct cgacggtgct ctggcccagc
      781 cgggcatcga cgccgcgatc gtgggaggcc cgatacatga gcgcggtgag tatctccgac
      841 gagccgccgc cgaggggttg gcaatcatct gccttcaccc cccgggggac gactccgagg
      901 cctattacca ggtgtccctg agccggtccg agaccggggc cgtgattgtg ccggacctgc
      961 ccctcaggat tcatcccggt gtggaggcga ttcgtcaagt catggaaggg ggcgagctcg
     1021 gtgcgttccg agcaattcgc tacgagttcc cctcccacgg cgagggaata gacctggccc
     1081 acgtcgtgtt tccccgttgt gtcgacgtcg tgcgggcgat tgtcggcgag atcgaggctt
     1141 tgaccgctac cggcgatcca ccgggaaccc gtcccgatat cgagctggtg gtccagcttc
     1201 gtgcagccaa agcccaacga gccgagctcc gtacctggtc gggccccgat gagcctgctc
     1261 ggcttacgct cctggggtcg caaggctcga caacgctcga attcgatact ctgctcgtac
     1321 agcccgcccg tctgatcagg cgcgtgacac atcaagcaga cgagctgatg gaactcgccc
     1381 catgggacgc ccaccatgcg attgtctcgg ttttggaggc gtcgcgggct gttcggccag
     1441 gtatcgatta tcccagtccg agcctccacg atgcgacgcg agccatggag ctgaccgagg
     1501 cgaccgtgcg cagcctccgt cgcggccgca ccgtcgacct gcattacgag ccgatcagcg
     1561 aggaagccac cttcaaatcg gtcatgacat cgacaggctg catgatcctt gtcggagcgc
     1621 tcttcgccgt gctcgtggcc ctctctggac cgccgctcgg attcaactgg acgatctata
     1681 tcgcctacct tattccgccg atccttgtaa tattcgtgat gatgcagacg ctccgattcg
     1741 ccgtgcgccg ccccgaccgg tcggacgagt atgccgcgcg agggtcgaca ttcgacgagg
     1801 cacgctctgg ccagggctag ctttttcgtc gatccgcaca tcggtcgctt gttctccaac
     1861 aatcaagggc ccgaagcgaa caacctgtcc tcgatgatcc gtcgccggcc cgttgtagag
     1921 tcggcacgcg cgcgcaatca gtcccggacc gagccagaca tgatcgatgt cgtagagagg
     1981 gaaccacgat ggaaacgtcc cgcgccaacc ggcagacgac cggctcgcta gtgtataacc
     2041 ctgatccatc aacgagtcga accctaggct tcggctggga gtattgaaat cgcccacgat
     2101 cacgtcgaac ggacgaccag cgtctgacgc ggcgcggcaa agatcggtga ttgccaccag
     2161 aaacgggagg cgcgatcgaa acggattgct ttggccatcg acgcaaagga gccgcaggcg
     2221 gcgaccccgt acgtcggccg tcacgctcat accggctccg ccgggcaagg caactcgccg
     2281 ctccaaccga atcggccagc gcgaaaacac gcccattgga taccagaaac tgctgcccgg
     2341 attgttctcg agataaaccg aggacgcggc gggaccgagg tcgtcggtga gccgagcgag
     2401 ccaatcggcg ggcgggagct cgctcaggac gatcaggtcg gggttgcccc tcagaatttc
     2461 agtccgctgc gcggcccacg tttgctggct gcggaacggc ccgcctcccc actggacgtt
     2521 ccaatggagc aaagtgacct cgggagtgtc gacgcggtgt actatcgccg gtcccaacgc
     2581 gatcatcgag accaatggca
//
LOCUS       DATAQL010000133         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1541101, whole genome shotgun sequence.
ACCESSION   DATAQL010000133 DATAQL010000000
VERSION     DATAQL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541101"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..859)
                     /locus_tag="VHS97_02195"
     CDS             complement(284..859)
                     /locus_tag="VHS97_02195"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3447032.1"
                     /translation="MAISIGIVVVVLIIAAIAFIATRPTNSRIQRSAQINAPADVVFS
                     IINDLRRWAEWSPYDKRDPNMKKTFEGPSAGPGAIYVWNGNSQVGEGRLTIVESKPGE
                     LVSMRLEFSRPFKCNNEVNFKIVPSDGGTRVSWIMDGKNTFMTKLMSIFMDMDKMVGT
                     DFEHGLANLNNVAQAETQRFGQSARADVKVG"
     gene            complement(885..1133)
                     /locus_tag="VHS97_02200"
     CDS             complement(885..1133)
                     /locus_tag="VHS97_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447033.1"
                     /translation="MNEDVSPTVFVALRPRGAKQTLGRLADFVATMWFLVSNEQVRNE
                     PPISEKETQYKSIPRFGMVADRFGVSWMILAMRSGNIY"
     gene            complement(1199..2254)
                     /locus_tag="VHS97_02205"
     CDS             complement(1199..2254)
                     /locus_tag="VHS97_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3447034.1"
                     /translation="MRCFVAGLVLLIWATTPAKAQVFDLVNLDHLNRKLRGRVVDYTQ
                     NHGADRRIYSPVLGKPRDLYVYLPPGYDPSVAYPLILFLHGADIDEHDFLDPGDLKAL
                     DWMISEGQIPAVVIAAPDGTYEGQNRVTSTHSLWVNGLGGRFEDHVVVEVVPFLMRTY
                     SIHPEREGHALLGISAGGFGAMAIALRYRNIFGAVATIAGPLNMRYDNCQGRYGDDFD
                     PATCRERTEYDPDMLIARFYFGLLKRRVKTYLEPVYGPGPDMIAKVARDNPADLLSAT
                     DLRPGELDIYVNYPARDNFNFDAQDQSFVWLAAQRGIAVDVTRDPRGHHNLSYIERAE
                     PPAYLWLGRHILPPARR"
     gene            2889..3455
                     /locus_tag="VHS97_02210"
     CDS             2889..3455
                     /locus_tag="VHS97_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447035.1"
                     /translation="MGHIAVPPPPFPLTASYLEAEFRDAERYRAEHGGLLPDNEFWRY
                     LETRWAVDPSRFDHWHPRIGPWLTENNQLTQEPQTVTPPPVSPPPIVTPPASPPPIFT
                     PPASPPPIVTPPTSPPPIVTPPTEPPPVETPPPASPPPIEIQPTGGGDPPPPPINGSG
                     GATPEPSSWVLLATSVVVTFMVSKFKKG"
     gene            complement(3650..4195)
                     /locus_tag="VHS97_02215"
     CDS             complement(3650..4195)
                     /locus_tag="VHS97_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3447036.1"
                     /translation="MGDRQAYSQSTLGPERTTYRTTVAEVSPCDGLIEANCPCQRPAQ
                     CPQGNRLLTRKENQKPANSRRTDFDFPVVDKERTWKILDVMAPIAKAHGCSPARLSLA
                     WLLAKPVVTSVIIGAKRLDQLQDNLAAVELTLTQDKLRQLDAVSALAPEYPGWVLALQ
                     GADRLEPVHRWERFREASNSR"
     gene            complement(4117..>4705)
                     /locus_tag="VHS97_02220"
     CDS             complement(4117..>4705)
                     /locus_tag="VHS97_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3447037.1"
                     /translation="FATNHLGHFQLTARLWKALKQARDARVVCLTSAGHCRAGVDLED
                     PNFVKRAYDKWAAYGQSKSANSLFAVELDRRGKEHGIRAFAVHPGGILTDLVRHMTDE
                     ELEPHGIVRENGVLVSPASLKNVEQGAATSVWCAVSSALSGKGGVYCEDCDIAPIVPD
                     DTKLLSGVRRWAIDKPTARALWDLSEQLTELQWPR"
BASE COUNT          968 a         1429 c         1354 g          954 t
ORIGIN      
        1 gccgtccgca cgcattgaag gcccgcggaa aaaatgtggt ggccgctctt ctcgagctga
       61 tcaacccagc cgatgcaggc acccacgacc gcgtccatct cgtcctgaga cagggcgtct
      121 agcttctttt cgtcttggta caccaagcac atgtactgca ttggttcctc gtcttcgcca
      181 ccgcatcgcg ttctgcgggc gattgcaagc gagtgctgtc aggaaatctc cagcagcccc
      241 tgcatttctc aaaccgaaag ctcagacgcg gtggcgggaa atattacccc actttcacgt
      301 cggctcgtgc actttgcccg aacctctgcg tttcggcttg ggccacattg ttgaggttgg
      361 cgaggccgtg ctcgaagtcg gtgcccacca tcttgtccat gtccatgaag atcgacataa
      421 gcttggtcat aaatgtgttc ttgccgtcca tgatccagct tactcgcgtt cccccatccg
      481 agggaacgat cttgaaatta acctcattgt tgcatttaaa ggggcgggag aactcgagtc
      541 tcatggagac gagttcgccg ggtttggact ccacaatcgt caagcggccc tctcctactt
      601 ggctattgcc gttccaaacg tagatggctc cgggtcccgc ggagggtccc tcgaacgtct
      661 ttttcatgtt cgggtcacgc ttgtcataag gggaccattc ggcccaccgg cgcaggtcgt
      721 tgatgatcga gaagacgaca tcggcgggcg cattgatctg cgcactccgc tggatgcgag
      781 aattcgtcgg ccgcgtcgcg atgaaagcga tcgctgcgat gatcaacacg acaacgacta
      841 ttccaatcga tatggccata agtacctccc ggcggtgaaa cggttcagta gatgttaccc
      901 gagcgcattg cgaggatcat ccatgacacg ccaaagcggt cggcaaccat gccgaatctc
      961 gggatggact tgtattgcgt ctctttttcc gagatcggcg gttcgttgcg aacctgttca
     1021 tttgacacta aaaaccacat tgtcgccacg aaatccgcca gacggccgag tgtttgtttc
     1081 gcaccccggg gtctgagcgc aacaaacacc gtcggtgaaa cgtcttcgtt caccgcgtgc
     1141 acatcgacga gagccgtgcc tcggttcgtt caaggcgctc aataaaccga aacaggattc
     1201 aacgccgagc cggcgggagg atgtgacggc ccagccacaa gtacgccggc ggttcggcgc
     1261 gctcaatata cgaaagattg tggtggcctc gaggatcccg cgtcacgtcg acggcaatgc
     1321 cgcgctgagc ggccagccag acgaaggatt ggtcctgggc gtcgaaattg aaattgtcgc
     1381 gggcagggta attgacgtat atgtccagct caccggggcg gaggtcggtc gcagagagga
     1441 ggtcggccgg attgtcgcgg gcgaccttgg cgatcatatc agggccagga ccgtacaccg
     1501 gttcgaggta agtttttacc cgcctcttga gtaaaccgaa gtagaagcgt gcgatcaaca
     1561 tatcaggatc atactcggtt cgttcgcggc aggtggcggg gtcgaagtcg tcgccgtagc
     1621 gaccctggca attgtcatag cgcatgttga gcggcccggc gatggtggcg acggctccga
     1681 agatgttgcg ataccgcagc gcgatcgcca tcgcgccgaa gccgcctgcc gagatcccga
     1741 gcaacgcgtg gccctcacgc tcgggatgga tcgagtacgt ccgcatcagg aacggcacga
     1801 cctcaacgac gacgtggtcc tcgaaacgac cgcccagacc gttaacccac agcgaatggg
     1861 tggaagtcac ccggttttgc ccctcatagg ttccatcggg cgccgcgatg acgaccgccg
     1921 ggatttggcc ctcgctgatc atccagtcga gcgccttcag gtcgccaggg tccaggaaat
     1981 cgtgctcgtc gatgtcggca ccgtggagga acaagatcaa tgggtaggcg accgagggat
     2041 cgtagcccgg cgggagatag acgtagagat cgcgcggctt ccccaggacg ggcgagtaga
     2101 tccgccggtc ggcgccgtgg ttctgggtat agtccaccac ccggccgcgg agcttgcggt
     2161 tcaggtgatc cagattgacc agatcgaaca cctgcgcctt agctggagta gtggcccaga
     2221 tgagcagcac cagacccgcg acgaagcatc tcatgaacca tccttggatc gagtggaatc
     2281 actgcgcagg atcactcaat tgatcggcta tgcgccaaca agcctttaac gtccttacgc
     2341 cggaacgcag gcacgatacg cttggactca accttgccat cgctttatcg cgccccactt
     2401 gacagttcct gattagctgc atatacatcg aagtgtggat ggaataggcg ataggccggt
     2461 tcgcactcaa tgtctttgca aagctctacg gcaagcgtcg cggcccgtca gttaattctt
     2521 gtcgcaagtt aggcaagcgt agtaacgtcg gctgtccggc tgccggctgg atcggatttg
     2581 ctgttgcgtt aatcggcatt ttattgtttc ctgtcactca gccgtccgca tggcggcgcc
     2641 catgaaacca tcatacgcgt cgctgcatca tgaaaggccc cggacatcgg caatctcctc
     2701 gactcgtttg tctctcgtga gactctcgcc tgtgatcatg gaacgggccg ctacgacgtc
     2761 gatgaaatca gccatgaccg gttgctcgtg gtgcgaacat cccgatgatc gggagaagtg
     2821 attgcaacga aggatcgcac agtgagtgtc gaccgtgaca ccctggacac cacggattgg
     2881 agtaaccagt gggacacatc gccgtaccgc cgccgccatt tccgctgacg gcgtcgtacc
     2941 tcgaggccga atttcgtgat gccgaacgct atcgagccga gcacggcggc ctgttacccg
     3001 acaatgaatt ctggcgatat ctcgagaccc gctgggcggt cgacccatcg cggttcgacc
     3061 actggcaccc gcggatagga ccatggctca ccgagaacaa tcaactgaca caagagccgc
     3121 aaaccgtcac gccaccaccg gtctcgccgc cgccaatcgt cacaccaccg gcctcgccgc
     3181 cgccaatctt cacaccaccg gcctcgccgc cgccaatcgt cacaccaccg acctcgccgc
     3241 cgccgatcgt cacaccaccc accgaaccgc cgccggtcga gacgccgcct ccggcgagtc
     3301 ctccgccgat tgaaatacag ccgacgggcg gcggtgatcc gcctccgccg cccatcaacg
     3361 gttccggcgg agccacgccg gagccatcgt cgtgggttct tctggcgacg tctgtcgtcg
     3421 taacgtttat ggtgtccaag ttcaagaaag gatgaccgcg tgccatcctg tgagcgggcg
     3481 aaccccggac agtaaagcgc tcgtcagcga tcaacagtgc cacaacgttc aagcaaaccg
     3541 ctccgccacg agcgcgatga tccagacgac actttgaact cgcccgagcg atggtcttgc
     3601 gacgcttggg caaaggcgga tttggtgagg ttgccgaatt cggtcaggat caacgtgaat
     3661 tgctcgcctc gcgaaaacgc tcccagcgat gaactggttc cagacggtcg gctccctgta
     3721 acgctagcac ccaacctgga tactcagggg caagagcgct gacggcgtcc aactgcctga
     3781 gcttatcttg ggtgagtgtg agttcgaccg ccgcgagatt gtcctgaagc tggtcgaggc
     3841 gtttcgcacc aatgatgacc gaggtcacca caggcttagc caagagccat gcgagcgaaa
     3901 gacgcgcagg gctacatcca tgtgctttgg cgatcggagc catcacgtcg aggatcttcc
     3961 aggtccgctc tttgtccacg acgggaaagt cgaagtcagt gcggcgcgaa ttcgcgggtt
     4021 tctgattttc ctttcttgtg agtaatcggt ttccttgggg gcattgtgcc ggtcgttggc
     4081 atggacaatt ggcctcgatt aagccgtcac acgggctcac ctcggccact gtagttcggt
     4141 aagttgttcg ctcaggtccc aaagtgctct ggctgtaggc ttgtcgatcg cccaccgtcg
     4201 aaccccggag aggagtttgg tgtcgtccgg cacaatcggc gcaatatcac agtcctcgca
     4261 atagacgccg cccttgccac tcagggccga gcttacggca caccaaacgg acgttgcggc
     4321 gccttgctcg acgttcttga gactcgccgg agacacgagc acaccgtttt cccgaacaat
     4381 accgtgcggc tccaattctt cgtcggtcat atggcgaacg aggtcggtga gaatccctcc
     4441 tggatggacc gcgaacgctc gaatcccgtg ctccttgccg cgcctgtcga gttcgaccgc
     4501 gaacagggaa ttcgctgact tggactgtcc gtatgccgcc cacttatcgt acgcccgttt
     4561 gacgaagttc ggatcctcga ggtcgacgcc agcgcggcaa tggcccgcgg aagtcaagca
     4621 aacgacccga gcatctcgcg cctgcttgag cgccttccac aaccgcgcag tcagctgaaa
     4681 atgacccaga tggttcgttg caaat
//
LOCUS       DATAQL010000134         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1694377, whole genome shotgun sequence.
ACCESSION   DATAQL010000134 DATAQL010000000
VERSION     DATAQL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694377"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VHS97_02225"
     CDS             <1..270
                     /locus_tag="VHS97_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447038.1"
                     /translation="RRRGLNEAQGIRVESVGVLAASGMIAGEALMGLVTAWFNYKYGK
                     VPQVFASPSYIFGILVLLLLGFVLVRVPLANAGRPEDPAPPAVIM"
     gene            complement(625..1890)
                     /locus_tag="VHS97_02230"
     CDS             complement(625..1890)
                     /locus_tag="VHS97_02230"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX3447039.1"
                     /translation="MTQRERDRLVVLKKAKKKLITQAQAAEEIGMTERQVRRLLRALK
                     KRGDRAVIHALKGKVSNRRMPDEERDKIVRILSDDLYHGFGPTLASEYLRRRHGIGIG
                     REALRLLMAEAGLWRPRRRRTDKVHLWRERRSRFGELVQWDTSDHDWLEGRGGSIHLI
                     HMIDDATSRVMARFVGSDSSEENMKLLWTWLERHGRPLAVYTDKDSMFHNAPKRVDGA
                     DLKQMQPTQIGRALAELGIAGIRAHSPQAKGRVERSFRTAQDRLVKGLRVERVRTLEA
                     ANEYLAREFLPWWEAHCTVRPAQPDDAHRPLGREHDLSAILSCVIERRVMNDYTFMLL
                     RNRYRIEPADIVPGLRGGILRIERRLDGSIAAAFQGRYLHFADCPAAVRQNARTPARV
                     PAANPTPRPKSTWMKNFHLKKSPPMQAAT"
     gene            complement(1993..2235)
                     /locus_tag="VHS97_02235"
                     /pseudo
     CDS             complement(1993..2235)
                     /locus_tag="VHS97_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2416..3795
                     /locus_tag="VHS97_02240"
     CDS             2416..3795
                     /locus_tag="VHS97_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3447040.1"
                     /translation="MGKRGSREKQDSIWIEAASLATPGAHPFYERLNKLLEKRGFDRF
                     AGEACGKFYSRTGRPGLPPGIYFRLLLVGYFEGIDSERGIAWRAADSFALRSFLGYEL
                     HQTTPDHTTISRTRRLIDVETHRSVFTWVLGVLAQEGLLKGNTVAIDGTTLEANAALR
                     SIVRRDTGAGYEEFLTGLAKESGIETPTREQLTKLDRKRPKKGSNEDWVNPHDPDAQI
                     TKMKDGRTHMAHKAEHAVDLETGAVVAVTLHPATAGDTNTVCETLAQCGEQIREVAVE
                     AGPETEQTLNPEGPAAAVLDKGYHSSDVLVVLKDVGVRTYCSEPARGRRKWIGKAEEQ
                     RAVYQNRRRIRGEHGKRLLRQRGERVERSFAHLYETGAMRRTHLRGHNNIIKRLLIHA
                     SAFNLALVMRKACGYGTPRGFGDAAQACILALRILCGLLRYAEFVESRRSEQVIGNIG
                     AHSNALSVS"
BASE COUNT          757 a         1250 c         1280 g          834 t
ORIGIN      
        1 cgccgccgcg gtctcaacga agctcagggc atccgtgtgg aaagcgtcgg cgttctcgcg
       61 gcttccggca tgattgcggg cgaagcgctc atgggcctgg tcaccgcctg gttcaactat
      121 aagtacggaa aggtcccgca ggtattcgcc agcccgtctt atatcttcgg cattctcgtc
      181 ctgctgctgc tcggatttgt gcttgtccgc gtgcctctgg ccaatgccgg ccgccccgaa
      241 gatcccgccc cgccggcggt aattatgtaa ctcacccccc tgtcaacgcc atttagaaat
      301 gtcctattcg ctgccacata gaaatgtccg gttttgaggg gggtggcggg agagtgggtt
      361 taaggtgaag gcgtccgaac ggggtcgcgc ccgaacggat gcccgaagcc gaggcgacgg
      421 agttggctcc gaaagcagtc ggcgcaaacc cctccgctgg ctcgccggtg gccggactgg
      481 ccacagtgag cgcggagggt tttgtttttg cgttttcaag caagctgctt ttggtgaaat
      541 tcccgagcgg aggcttcggg gcagtgaacg cgagagcccg ccgggcggtg tttgcccggt
      601 gctccctgtg gattgtgtct gcggctatgt cgcagcctgc atcggcggcg attttttcag
      661 atggaagttt ttcatccacg tgctcttggg ccggggcgtg ggattcgcag ccgggacgcg
      721 agcgggcgtt cgggcgttct gccgcacggc ggccgggcag tctgcaaaat gcagatagcg
      781 gccctgaaac gcagccgcga tgctgccgtc gagacgccgc tcgatccgca gaatcccccc
      841 gcgcagcccc ggcacgatat cggcaggctc gatcctgtac cggttccgca gcagcatgaa
      901 tgtgtaatcg ttcatcaccc tgcgctcgat cacgcagctc agaatggctg acagatcgtg
      961 ctccctgccg aggggccggt gcgcatcgtc gggctgcgcc ggcctgacgg tgcagtgcgc
     1021 ttcccaccag ggcagaaact cgcgcgccag atactcgttg gcggcctcca gggtccgcac
     1081 gcgttcgacc cgcagcccct tcaccagccg gtcctgagct gtccggaagc tgcgctcgac
     1141 ccggcctttc gcctggggcg aatgcgcgcg aatccccgct atgccgagct ccgccagagc
     1201 ccggccgatc tgcgtcggtt gcatctgctt cagatcggcg ccgtcgactc gcttcggcgc
     1261 attgtgaaac atgctgtcct tgtcggtgta gacggccagc ggccggccgt gccgttccag
     1321 ccaggtccac aacagcttca tgttctcctc gctggaatcg ctcccgacga aacgcgccat
     1381 cacccggctg gtcgcgtcgt cgatcatgtg gatcaggtga atcgagccgc cacgcccttc
     1441 gagccagtcg tgatcggagg tgtcccactg aaccagttcg ccgaaacggc tcctgcgttc
     1501 ccgccacagg tgaactttgt ccgtcctgcg gcgccgtgga cgccagaggc cggcttccgc
     1561 catcaacagc cgcagcgcct ctctgccgat gccgatgcca tgcctgcggc gcagatactc
     1621 gctggccaga gtcggcccga agccgtggta aagatcgtcg gacaggatgc ggacgatctt
     1681 gtcgcgctcc tcgtccggca tgcggcgatt cgagaccttg cctttgagcg cgtgaatcac
     1741 cgcccggtcc ccccgcttct tcaatgcacg caacagccga cgaacctgac gctcggtcat
     1801 gccaatttct tcggcggcct gcgcctgcgt gattagcttc tttttcgctt tcttcagtac
     1861 aaccagccgg tcccgttcgc gctgggtcat ggtgagaact ccctgttctt ccacgcttcc
     1921 agcctgacct tgtcaaccgg acatttctat gtggcacgaa taggacattc tcacgtggcg
     1981 gcgacaggac accaccggtc cttgcttttg catcctcggc agcgcataat tgcgcacctg
     2041 ttcgagcaac ccttcgtcgc tccacggcgc cttcgctacc agatgatgga gactttggcg
     2101 catggattga acgttctccg gagccagacg cgccgccatc ggctcgatgc tcttgcgttc
     2161 gcccggcagc agcaacccct tgcaatagct cttcaacggc tcttgccggt cctgatgccc
     2221 cgcggctgtc gccaagccct ccatatacgc cgtgaagcgt ttctcttgtg ggcctggcgc
     2281 ggagcggcgt tttcccacaa aacaattcta tatcaaaatg ttgttttatg acccagtagt
     2341 actaggggcc cggggtgcaa cccgattttc ctcttggccc tgtgcaagga atgaccgtag
     2401 tatggaggca tgtcgttggg caagcgcggt tctcgggaga aacaggactc gatctggatc
     2461 gaagccgctt cgctggccac cccgggggct catcctttct acgagcgcct gaataagctt
     2521 ctggaaaaac gtgggttcga ccgcttcgcc ggcgaggcct gcgggaagtt ctattcgcga
     2581 accggtcgtc cgggactgcc gccaggcatt tattttcggt tgctgctggt cgggtatttc
     2641 gaggggatcg attcggagcg cggcatcgcc tggcgggctg cagactcctt tgcgctgcga
     2701 tcgtttctcg gctatgaact gcatcaaacg acgccggatc acacgaccat ctcgcgcacg
     2761 cgtcgactga tcgatgtgga aacgcatcgg agtgtattca cgtgggtgtt gggcgtgctg
     2821 gcgcaagagg gtctgctgaa agggaatacg gtcgctattg acgggacgac gcttgaggca
     2881 aatgccgcgc tgcgctcaat cgtgcggcgc gataccggcg ccggttacga agagttcctt
     2941 accggcctgg cgaaggaatc gggcatcgag acgcccacgc gcgagcagtt gacaaaactg
     3001 gatcgcaagc gaccgaaaaa gggttccaac gaggactggg tcaatccgca tgatccagat
     3061 gcgcagatca caaagatgaa ggacggccgc acgcacatgg cacacaaggc cgagcacgcg
     3121 gtcgatctgg agaccggcgc agtggtggcc gtgacactgc acccggcgac ggcgggcgac
     3181 acgaatacgg tctgcgaaac actggcgcag tgcggcgagc aaatccgcga ggtggctgtt
     3241 gaggccggcc cggaaacaga acagaccttg aatccggaag gcccggccgc ggcggtgctg
     3301 gataagggtt atcacagcag cgatgttctg gttgtactga aggacgtcgg cgtacgaact
     3361 tactgttcgg agccggcgcg gggacgccgc aagtggatcg gcaaagcaga ggagcagcga
     3421 gcggtttatc agaaccggcg gcgcattcgc ggcgaacacg gcaaacggct gttacggcaa
     3481 cggggagaac gggttgaacg cagcttcgcg catctgtacg aaacgggcgc gatgaggcga
     3541 acgcatctac gcgggcacaa caacatcatc aaacggctgc ttattcacgc cagcgcattc
     3601 aatctggcgc tggtgatgcg taaagcctgt ggatatggaa cgcccagggg attcggagat
     3661 gcggcacagg cctgcattct cgcgttgcgc attctttgcg gcctgctccg atacgcggaa
     3721 tttgtggaat cacggagatc agaacaggta atcggtaaca ttggcgctca cagtaacgcc
     3781 ctttcggtta gctgaactat cgcttcagat ttctcccctc acgatccaag aacagttcaa
     3841 acccgggctg ctagggcttg cgcccactat cagtaccagc ggaggaacgc ttgcaggggg
     3901 gattaactat tactacgcgg ttagcgcagt ggatagcggc gggggcgaga gtccgctttc
     3961 cttcgtagct caggccagtc cgaacgcggc gtcgaatgct aattcggtgg cgcttggcgg
     4021 gatcgggttg ccggccggta cggtaagttt caatgtttat cgcggctcgg cgccgcaaca
     4081 attgcttcgg attgcttcga atcagccgcc ggcaacctca t
//
LOCUS       DATAQL010000135         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1846859, whole genome shotgun sequence.
ACCESSION   DATAQL010000135 DATAQL010000000
VERSION     DATAQL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846859"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VHS97_02245"
     CDS             complement(<1..1118)
                     /locus_tag="VHS97_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3447041.1"
                     /translation="MFAATLLTAQPFKATKTTSGAPAATVQTYPTSDLSRTGIFYAGG
                     KYVGEPGKEVMGGSAYVEVWVPKQIKHPYPIIYIHGAGQTATDWLQTPDGRAGWAYYF
                     AKLGYVQYMVDSPARGRSPYVPDHDGNLTIRNVANLEATFTASAKKGDFPRAHLHNQF
                     PGTGLMGDLAFDEFAKTQVQFLAGGGPASQDELSRDAFIALLDMIKSPVIILSHSQGG
                     TVGWLMADARPNQVKGIVTVEPAAPPIKGVDTAKVAYNAGGGGGGLSWGVTSSPITYD
                     PPIKSPSELQVVVEEKSDIPGDVVPCYVQKEPARKLVNLEKIPVVYLSSEGGYHREFD
                     HCLAKWLNQAGVKTQFVRMEDVGLRGNGHEMMLEKNSD"
BASE COUNT          304 a          469 c          490 g          327 t
ORIGIN      
        1 tcgtcgctgt tcttctccag catcatctcg tggccgttgc cgcggagccc gacgtcttcc
       61 atgcgcacga actgagtttt cacaccggcc tggttgagcc acttggcgag gcaatgatcg
      121 aactcgcggt gatagccgcc ctccgacgat agatagacaa caggaatttt ttcgagattg
      181 acgagcttcc gcgcaggctc cttctggaca tagcacggaa ccacgtcccc aggaatatcc
      241 gacttctctt cgaccacgac ctgcaattcg gatggggact tgatcggagg atcgtaagtg
      301 atcgggctcg aagtaacgcc ccagctcaga ccgccgcctc cgccccccgc gttataagcc
      361 actttcgccg tgtcgacacc tttgatcggc ggcgcggccg gttccacggt cacgatgccc
      421 ttcacctggt tgggacgcgc gtcggccatg agccatccga cggtgccgcc ttgcgaatgg
      481 gacaaaatga tgaccggaga tttgatcatg tccagcagcg cgatgaaggc gtcgcggctc
      541 agctcatcct gtgatgcggg cccgccgccg gccaggaact gcacctgcgt cttggcaaac
      601 tcgtcgaacg cgaggtcgcc catcaggccc gtcccgggaa attgattatg cagatgagcg
      661 cgcgggaagt cacccttctt cgccgatgcg gtgaaggtcg cttccagatt cgcgacgttg
      721 cggatcgtca ggttgccatc gtgatcaggc acataaggcg aacggccgcg ggccggtgag
      781 tcgaccatgt actgcacgta gccgagcttg gcgaaataat aggcccatcc cgcacggccg
      841 tcgggagtct gcaaccaatc ggtggcagtc tgtccggcgc cgtgaatata aattatggga
      901 tagggatgct tgatctgctt gggtacccag acctcgacgt aggccgaacc gcccatcact
      961 tctttgccgg gctcgccgac gtatttgccg ccggcgtaga agataccggt gcgtgaaagg
     1021 tcgctggtgg gataagtctg gaccgttgcc gcaggtgccc cgctggtggt tttcgtggcc
     1081 ttgaacggct gggcggtgag taaggtggcg gcgaacatcc cgacgactcc ggccaccggc
     1141 actgatgtcg gcggtcgcat cttaggaaag tgcatagcta attgtcgtac aaggttaccc
     1201 cgttgtgcgc tcagcgcgca ggtggatgga tgatcggaat ggcttcgcgg cggcctcgac
     1261 ggtaaacgcg gaagtcggcg agatcggtcg tgagtaccga gtgtctcgga tgtagttcgc
     1321 tcagccggat caggcacaga tccgcgaggt ccggctacca agatgaaacg cggcttcggc
     1381 gaggacggcg tcacaagtca aacgtggctc cgtcacccgt gtcgcgattt caagcgccca
     1441 cccgtgatgc tgatcgttgc gtttgccgaa tgccaccaga aaaccggtgt cggcgctcgc
     1501 cttcatttct tggagaatcc cttacgttcc gaaaggtctt ttggtccgcc gattgtccca
     1561 gcaagccgta ggaagggacg agcatctgcg
//
LOCUS       DATAQL010000136         8566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1541589, whole genome shotgun sequence.
ACCESSION   DATAQL010000136 DATAQL010000000
VERSION     DATAQL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541589"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VHS97_02250"
     CDS             <1..356
                     /locus_tag="VHS97_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX3447042.1"
                     /translation="ELGREVMHNRTIAQLGSWQPAPTRKSFRDRPATEAPAAEIPMSE
                     HGALRQALRAWRSRKSQEMGVPPYTLFWDRTLDELCMRRPSTQDDLAGVWGIGEEKRR
                     KFGAEILAVIAGCAE"
     gene            468..1901
                     /locus_tag="VHS97_02255"
     CDS             468..1901
                     /locus_tag="VHS97_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447043.1"
                     /translation="MVHIDDEIGSSSRATQAPPRWAMTTFVALFAMNMLDYLDRNLLM
                     SMQPQIKEDLRISNFQWGLLTSIFLVSYSVFGPFMGWLGDRLRRTWLLGLGVGVWSLA
                     TIGSGLARTYGHMAVARSVLGIGEATYGVIAPTILLDLFAREQRARLMSAYYVAMPLG
                     SALGIGLGSVIASKLSWHLAFFMVGAPGVAAALLVLFLPEPVRGASEGVDTERLKALE
                     RAGARREDYLALSKNASYVYAVLGMAAYTFAIGGMLVWVPIYLFTTRGFDQARAGTIL
                     GVITLAAAVLGMSAGGWLADRLAKTKPEALFLVPAVAMFGSIPFVVAALLLNAEPAIF
                     AAIFLAEALMFVNTGPCNAIIANVVQPNLRAAAYSIAIFAVHFLGDIWSPSLIGKVSD
                     LFGDAEMMAGRLGRVLSSIGAVPTQVNGHSPENIVAGLFVVVPALLLSGILLLAGARY
                     LRREMAQMQSRLEAAPESAATAEATNS"
     gene            complement(1948..3087)
                     /locus_tag="VHS97_02260"
     CDS             complement(1948..3087)
                     /locus_tag="VHS97_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3447044.1"
                     /translation="MIERITRLKREAGTHSPSAWTLAQRIPELRLKVDACFLSNPYAT
                     NLFLMHFHREVIRPRRLRALVELYPSQNRVIATKLGRVLELDDDSIFVGNGAAEIIQA
                     ILHRFTRGKILVNLPTFSPYYEFSRPDTQVIYNVLRKENNFQFDPTAYLDLVRRERPD
                     TVVLINPNNPDGGYVPYAVMRRMLDELRTVPNVIVDESFIHFACEGDGYIYRSVADQV
                     GRLPNLTVVKSMSKDFGVAGIRAGYAVMAPDRVRTLLDNGYLWNSSGIAEYFFDLYSR
                     PDFQAQYERERIRFIRNSRRFFAALGKIDGLHVYPTHANFALAELRGGLSADELVCRL
                     LIRRGIYTRTCDDKKGLEAGKFVRIAARNRPENAYIVRSLRRMIG"
     gene            3473..4021
                     /gene="fae"
                     /locus_tag="VHS97_02265"
     CDS             3473..4021
                     /gene="fae"
                     /locus_tag="VHS97_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEX3447045.1"
                     /translation="MSERIGLRTGEALVEGSLDYLCAEPEIVIGELDGPVGHALANLI
                     GDQVKGHSRVFAILSSDVQVRPTTVMVSKVTVKSGKYTNILMGTVQAAIANGVLDAVR
                     AGDIPKDKVNDLGIIYSVWLDPSVTEVDDLDHNALFRVHREATAKVIKKAMRNEPTID
                     WLLENQDKITHYFHELGLKGEL"
     gene            complement(4106..4651)
                     /locus_tag="VHS97_02270"
     CDS             complement(4106..4651)
                     /locus_tag="VHS97_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447046.1"
                     /translation="MNAKAIMTLALRIMGFWVLFSAVAAMVNFAAFLTSSIPRVAGPQ
                     GELGFLISSGFGLIAYASFAAGLLLFAPAIASWFSTEPAPPIPVATDRRVDVRDVYII
                     AARLLGLYSLSSGVPTAQRLAQLILDYRFEWGRAGEFIWAEFVQASIYLVGAALLIFC
                     AAGIADVFSRAHGMSERPMAG"
     gene            complement(4654..5568)
                     /locus_tag="VHS97_02275"
     CDS             complement(4654..5568)
                     /locus_tag="VHS97_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447047.1"
                     /translation="MRRPGPCIDTPHPLHAGPAIEAARAGVHVLVEKPLAASLNDCDL
                     MLAAARDNRTKLGVISQRRWYEPVRRMKAAIDADKIGRPVLGVFTMYSWRDPSYYQSD
                     PWRGRWDTEGGGVLVNQSPHMIDLLAWLMGDEVAEISGYWANLNHPSVAVEDSAVAIL
                     RFRNGGLGSIVASLSQKPGVYTKVHIHGSNGASVGVETDRGATFIAGVSAISEPPLND
                     LWTIPGEEDNLARFQAEDRARFQLIDAVNHYHALQIQDFLRAILEDRPPLVTGEDGRR
                     VVALFQAIYQANREGTSIKCSRDQCREI"
     gene            complement(5451..5753)
                     /locus_tag="VHS97_02280"
     CDS             complement(5451..5753)
                     /locus_tag="VHS97_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447048.1"
                     /translation="MDRVRTALIGCGKVGQIHALALAALPESEFVAACDVDLPRATTF
                     ATRFGVRPYGDVARMLEECGVQALASTPRTRSMPGRRSRPPGQVSTFWSKNRWRPA"
     gene            complement(5763..6662)
                     /locus_tag="VHS97_02285"
     CDS             complement(5763..6662)
                     /locus_tag="VHS97_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3447049.1"
                     /translation="MALKIAAFPKCFIDQIAGDRSMSVFDWIEMARSLDADGLEMYDG
                     FFTSLEPSYLDQVGEAIAAAGFAMPMLCCSPDFTNPDPDARKRAVDREAELIQVARRL
                     GGPRTVCRVLSGQRYPDISRHQGLEWVVDCINQVLGAAREHDIVLGLENHYKDGFWTY
                     PEFAQKMDLFLELVDSIPNGDHFGVQYDPSNAIVAGDDPIALLRAVAPRVVSMHASDR
                     SLAEGATLEDLRQTDGTLGYSPELRHGVTGKGLNDYNSIFRILAEHGYQGWVSIEDGM
                     NGMDEMAESLAFLRRMSLQYFPD"
     gene            6873..8018
                     /locus_tag="VHS97_02290"
     CDS             6873..8018
                     /locus_tag="VHS97_02290"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX3447050.1"
                     /translation="MMFETARVGTGDRPWHRAHVVAPGVLLLLSCLVSTSAAQDRQPA
                     PKDAVRTSYPRINLSTGYEVISSWPAGHKDLAWGAVAGIAIGPEDQVWTFNRGTVPVQ
                     VYSANGELLRSWGEGLFREPHQVRIDRQGDVWLVDSGQHCVRKYTPDGKLLLTLGTPG
                     EPGEDSTHLNRPTDVAITSAGDLFVTDGYGNNRVVHFDRQGRFVTAWGSLGVGPGQFS
                     LPHSIAVDSQGRLYVADRNNARLQVFDQTGRFLDEWRDLLVPWHIVITDRDEIYVCGS
                     SPMRWPRIPIPGLVVGVPPKDQLVMVFAPDGRVLRLYTFLKGRQPGELDWVHALAVDR
                     RGNLYLGDIQGRRAQKFIRLEPAVRDGDVAKKSLPRRDEPVERAGKP"
     gene            complement(8069..8386)
                     /locus_tag="VHS97_02295"
     CDS             complement(8069..8386)
                     /locus_tag="VHS97_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447051.1"
                     /translation="MDTLDHIFGAMDNWSTCVTFGEGQTVVGKGEALADLKRVVSVPD
                     RGAVVVPRLSKKKPASGIAGLTTGAHLVARGLFGCRAVGRKLGVITVTKRCAVQQRLS
                     ARA"
BASE COUNT         1628 a         2565 c         2662 g         1711 t
ORIGIN      
        1 ccgaactcgg ccgcgaggtc atgcacaatc ggaccatcgc tcagctggga tcctggcagc
       61 cggccccgac caggaaatcg ttcagggata gaccggcaac ggaagccccg gctgccgaaa
      121 ttcccatgtc cgagcacggt gcgcttcgcc aggcgctgcg ggcgtggcgc tcgcgaaaat
      181 cccaggaaat gggcgttccg ccgtacacgc ttttctggga tcgtacgctc gatgaactgt
      241 gcatgcggcg accgagcact caggacgacc tcgccggcgt ctggggtatc ggtgaagaaa
      301 agcgtcgcaa gtttggagcc gagattctcg cggtgatcgc cgggtgtgcg gagtgattct
      361 gctcttaccg gtggacgttc cctgagccgt tttcgtaaca tcaccctgcc tgggcggtcg
      421 gagccgccct cgcgtgttag ggcgtcctgg caccaaggag gcacgacgtg gtgcacattg
      481 acgacgagat cggatcatcg tcgcgcgcca cgcaggcgcc gccgcggtgg gccatgacaa
      541 cgtttgtggc actctttgcc atgaacatgc tcgactatct cgatcgcaac ctgctgatgt
      601 cgatgcagcc gcagatcaag gaagatctgc gtatctcgaa tttccagtgg ggcctcttga
      661 ccagcatctt cctggtgagc tactcggtgt ttggcccgtt catgggctgg ctgggcgatc
      721 ggctccggcg tacctggctc ttgggattgg gggtcggggt ctggagcttg gcgacgattg
      781 gcagcgggct ggcgcgcact tacggtcaca tggcggtggc gcggagcgtg ctggggatcg
      841 gcgaggccac ttacggcgtc atcgcaccga cgatcctcct ggaccttttc gcccgcgagc
      901 agcgggcccg gctgatgtcg gcgtattacg tggcgatgcc gctgggtagt gcgctaggga
      961 tcggcttggg ctcggtcatc gcgtccaagc tgagctggca cctagcgttt ttcatggtcg
     1021 gggcgccggg cgttgccgcg gcgctcctgg ttctgttcct tcccgagcca gtgcgcgggg
     1081 cgagcgaggg ggtcgatacc gagcggctca aggctttaga gcgggccggc gcccgccgcg
     1141 aggattattt ggccttgagc aagaatgcgt cgtacgtcta tgcggtcctc ggcatggccg
     1201 cctacacctt cgccatcggc ggaatgctgg tctgggtacc catttacctg ttcaccaccc
     1261 gcggctttga ccaggcgcgg gcgggaacca tcctgggggt catcacgctg gcggcggccg
     1321 tgctgggaat gtcagctggt ggctggcttg ccgaccggct ggccaaaaca aagcccgagg
     1381 ctctgtttct ggtgccggcc gtcgcgatgt tcggctcgat cccatttgtc gtggcagcgc
     1441 ttttgttgaa cgccgagccg gcgatcttcg cagcgatctt tcttgccgag gccttgatgt
     1501 ttgttaacac agggccgtgc aatgcgatca tcgccaacgt ggtgcagccc aacttgcggg
     1561 ccgcggcgta ttcgatcgcg atcttcgcgg tccatttcct gggtgacatc tggtccccgt
     1621 cgctgattgg caaggtttcc gacctgtttg gcgatgcgga gatgatggcc ggccggctgg
     1681 gccgggtgtt gagctcgatc ggagcggtgc caacccaggt aaacggccac tcgcctgaaa
     1741 atatcgtcgc cggtctgttc gtggttgtgc cggcgctttt gctatcgggc attctcctcc
     1801 tggccggcgc gcgctacctg cggcgggaaa tggctcagat gcagtccaga ctcgaggcag
     1861 cgcccgagtc ggccgccact gcggaagcga cgaattcgtg atctcggggc aatacgcctg
     1921 ggtcaccctt tgcaagtaag cggcgtttca gccaatcatt cggcggagcg agcgcacgat
     1981 gtaggcgttt tcggggcggt tgcgggcggc gatccgcacg aacttgccgg cttcgagccc
     2041 ttttttgtcg tcacaggttc gcgtgtaaat tccgcgccga atcagcaagc ggcacacaag
     2101 ctcgtcggcg ctcaagccgc cgcggagctc ggccagggca aaattggcat gggtaggata
     2161 aacatgcagc ccgtcgatct tgccgagggc ggcgaaaaac cggcgcgagt tacggatgaa
     2221 acggatgcgc tcgcgctcat attgcgcctg gaaatccggc cgggaataca aatcgaagaa
     2281 gtattcggca atgcccgagg aattccagag ataaccgtta tcgagcagtg ttctcacgcg
     2341 atcgggcgcc atcaccgcgt atccggcccg gattccggcc acgccgaagt ccttggacat
     2401 gctcttgacg accgtcaggt tcggcaatcg gccgacctga tcggcgacgc ttctgtagat
     2461 gtaaccgtct ccctcgcagg cgaagtggat gaagctctca tcgacgatca cgttggggac
     2521 ggtccggagt tcatcaagca tgcgccgcat cactgcgtag gggacatagc cgccatcggg
     2581 gttgttgggg ttgatgagga cgaccgtgtc gggccgttcg cggcggacca gatcgagata
     2641 agccgtcggg tcaaactgga aattgttctc tttgcgtagg acgttgtaga tgacctgtgt
     2701 atcgggccgg ctgaactcgt agtagggcga aaatgttggc aggttgacca ggattttccc
     2761 ccgcgtaaac cgatgaagga tcgcctggat gatttcggcg gcgccgttgc ccacgaagat
     2821 cgaatcgtca tcgagctcca gcacgcggcc tagcttggtg gcgatcacgc ggttttgcga
     2881 cggataaagc tcgaccaggg cgcggagccg cctaggacgg atgacctcgc ggtggaagtg
     2941 catcaagaac agattcgtcg cgtatggatt ggacagaaaa caggcatcca ctttgagccg
     3001 gagctcagga atgcgctggg ccagtgtcca ggcgctgggg gaatgagtgc ccgcctctct
     3061 tttgagccgc gtgatccgct cgatcaacgc ggcttcgcgt gggctctggc cgatccgagc
     3121 gagtgatttc cgtctctgct gctccagcca ccttgccgaa cgccccagat caaacggttt
     3181 aaatcttaat ccatgttcgc gaggacgctc gacaatcact tcaacctcat cgagttggca
     3241 tgaccactgt ggcgggaccc atcgtcgtat attattgttc atctagacgc tacgacgact
     3301 tcacaaaatc ttagcacatc gtagatccgc ggggggtgcg aggcaaggtc ctagtgcaag
     3361 tgggctccgg acggttttct tatcctgtct ggcgcacaat actagggcgc gcgggcttac
     3421 tttacggttt gcaccaaaat tgacctcatc acaagagaca cgaggtgaaa ccatgagcga
     3481 gcggatcgga ctgcgaacgg gtgaggcctt ggtcgaaggt tccctggact atctctgcgc
     3541 tgaaccggaa atcgtgatcg gcgagctcga cggcccggtc ggccacgcgc ttgctaacct
     3601 gatcggcgac caggtcaaag ggcattcgcg cgtgtttgcg atcttgagta gcgacgtgca
     3661 ggtccggccg accaccgtga tggtgagcaa ggtgacggtc aagagtggaa aatacaccaa
     3721 cattttgatg ggcacggtcc aggcggcgat cgccaacggg gtactcgacg cggtgcgcgc
     3781 cggcgacatt cccaaagaca aggtcaacga cctggggatt atctactcag tctggctcga
     3841 tccctcggtg acggaagtcg acgatctgga tcacaatgcc ctttttcgtg tgcaccgcga
     3901 ggcgactgcc aaggttatca aaaaggcaat gcgcaacgag ccgacgatcg attggctgct
     3961 ggagaaccag gacaaaataa cgcactattt ccacgagctg gggctcaagg gggagctgta
     4021 ggctcacggg ttctgctcaa tccatatccg ccggcagggc accgcggagc ggcgtgctca
     4081 cgtttcattc gattggacgc ttagctcacc ctgccatcgg ccgctcggac atgccatgag
     4141 ccctcgaaaa gacatccgcg attccggcgg cacagaagat taacaaggcc gcgccgacca
     4201 ggtagatcga tgcctggacg aactccgccc agataaactc ccccgcccgt ccccactcga
     4261 acctgtagtc gaggatcaat tgtgccagtc tctgggccgt cgggacgccg gacgacagcg
     4321 aatagagccc cagtaatctc gccgcgatga tgtagacgtc ccgcacatcg acccgacgat
     4381 ccgtggcgac aggaattggg ggggctggtt cggttgagaa ccacgatgcg atcgctggtg
     4441 cgaacaagag caaccctgcc gcgaaggacg cgtacgctat caacccgaaa cccgaggaga
     4501 taaggaaacc caattccccc tgcggcccgg cgacacgggg gattgacgag gtgagaaagg
     4561 ccgcgaagtt gaccatcgca gcgaccgcac tgaacaagac ccagaatccc ataatgcgca
     4621 acgccaacgt catgatcgcc ttggcattca tcgctatatc tccctgcact ggtccctcga
     4681 acacttgatc gacgtcccct cgcggttcgc ctgatagatg gcctggaaga gcgccacgac
     4741 gcggcggccg tcttcgccgg ttacgagtgg gggacgatcc tccaatatcg cccgcaggaa
     4801 atcctggatc tggagcgcat ggtagtgatt caccgcgtcg atcaactgga agcgagcccg
     4861 atcctcggcc tggaaccgcg ccaggttgtc ttcctcgccg gggatggtcc acaggtcgtt
     4921 taaagggggc tcggagatcg ccgatacgcc ggcgatgaag gtcgcgccgc ggtcggtttc
     4981 gacgccgacg gaagcgccgt ttgatccatg aatgtgcacc tttgtgtaga cgcccggctt
     5041 ttgcgacaga ctggcgacga tcgagccgag ccccccattg cggaaccgga ggatcgcgac
     5101 ggcgctatcc tcgacggcga ccgacggatg gttgaggttg gcccagtagc cgctgatctc
     5161 ggcgacttcg tcgcccatca gccacgcgag caagtcgatc atgtgcggcg actggttgac
     5221 gagcactccg cccccttcag tgtcccagcg cccgcgccac ggatcggatt ggtaataaga
     5281 tgggtcgcgc cagctataca tcgtgaatac gccgagcacg ggccggccga tcttgtcggc
     5341 gtcgatcgcg gccttcatgc gccggacagg ctcgtaccac cgccgctgac tgatcacgcc
     5401 cagcttggtt cggttatctc tcgccgcggc gagcataagg tcgcaatcat tcaagctggc
     5461 cgccaacggt ttttcgacca gaacgtggac acctgccctg gcggcctcga tcgccggccc
     5521 ggcatggagc gggtgcgggg tgtcgatgca agggcctgga cgccgcactc ctcgagcatc
     5581 cgcgccacgt cgccgtaggg tctcacgccg aagcgcgtgg caaaggtcgt tgcacgcggt
     5641 aaatcgacgt cgcatgcagc cacgaactcc gattcgggca aggccgccag tgccagagcg
     5701 tgaatttggc caacctttcc acaacctatt agtgctgttc gcacccggtc catcggtgat
     5761 cctcaatcgg gaaaatattg aaggctcatc cgccgcaaga acgccagcga ctcggccatc
     5821 tcgtccatcc cgttcatgcc gtcctcgatg ctgacccaac cttgataacc gtgctcggcg
     5881 aggatccgga agatagagtt gtaatcgttg agtcctttgc cggtcacgcc gtgccggagc
     5941 tcgggtgaat aacccagcgt accgtcggtc tggcggaggt cttcgagcgt cgccccctcg
     6001 gccagggagc ggtcactcgc gtgcatgctg acgacccgcg gcgcgacggc tcgcaagagc
     6061 gcgatcgggt cgtcgccggc gacgatcgcg ttcgacgggt cgtattgcac tccaaaatga
     6121 tcgccgttgg ggatcgagtc gaccagctcg agaaacagat ccatcttctg ggcgaattca
     6181 ggatacgtcc agaagccatc cttgtagtga ttctccagtc ccaggacgat gtcgtgttca
     6241 cgggctgccc ccaagacctg attgatgcag tcgacgaccc actcgaggcc ttggtggcgg
     6301 ctaatgtccg ggtacctctg gccggaaaga acccggcaaa ccgtgcgcgg tccgcccagc
     6361 cggcgggcta cttgaatcaa ctcggcttcg cggtccacgg cgcgcttccg cgcatcgggg
     6421 tcggggttcg tgaaatcggg cgagcagcaa agcataggca tggcgaagcc cgccgcggcg
     6481 atggcctcac ccacctgatc aagatacgag ggctcgagac tggtgaaaaa gccgtcgtac
     6541 atctccagcc cgtcggcgtc gagcgaccgc gccatctcga tccagtcgaa gaccgacatc
     6601 gatcgatcgc ccgcgatctg gtcgataaaa cacttgggaa aagccgcgat cttcagggcc
     6661 atgccggtgt ctcctcgcgt gaaaggcggc cgtgcagccg gttgtcgaga gtcgattgga
     6721 agactaagat ggataagatg ccgacatcaa ggcaaacaaa gcctattttt tttccccccc
     6781 cggcggccat gcgtcgcgca acctgcggga catgtggctc tttagaccat cgactctctc
     6841 aggcagggcg tggtcgccct cgagtgttct tcatgatgtt tgaaactgcc cgcgttggta
     6901 ccggcgatcg tccttggcat cgcgctcacg tcgtggctcc aggcgttctt ttgcttcttt
     6961 cttgtctggt ctcgacatcg gcggcccaag atcgccagcc ggcgccgaaa gatgcggtgc
     7021 gcacttcgta ccccaggatc aacctatcca ccggctacga agtgatttcg tcgtggccgg
     7081 ccggtcacaa ggatctcgcc tggggggcgg tcgccgggat tgcgatcggt cctgaggacc
     7141 aggtctggac gtttaatcgt ggaacggtgc ccgtgcaggt ctacagcgcc aacggcgagc
     7201 tgcttcgttc ctggggcgag ggacttttcc gagagcccca tcaggtgcgc atcgaccgcc
     7261 aaggcgatgt ttggctggtc gacagcgggc agcattgcgt gcggaaatat accccggacg
     7321 gaaaactcct gcttactctc ggaacaccgg gagagcctgg tgaagattca acgcacctga
     7381 accggcccac ggatgtggcg atcacgagtg cgggcgatct ctttgtaacc gacggctatg
     7441 gtaacaaccg ggtcgtgcat ttcgatcggc agggacggtt cgtgacggcc tggggaagct
     7501 tgggggttgg tcccggacag ttcagcttgc cgcattcgat tgccgtcgat tcccagggca
     7561 ggctgtacgt ggcggatcgg aataacgccc gtttgcaagt tttcgatcag accggccgtt
     7621 tcctcgatga atggcgcgac ctcttggtcc cctggcacat cgtcattacc gaccgggacg
     7681 aaatctatgt gtgcggttct tcgcccatgc gctggcccag gattcccatt ccggggctag
     7741 tcgtgggcgt tccgcctaag gaccagcttg ttatggtttt tgcgcccgac ggccgcgtgc
     7801 tccggctata cacatttctc aaaggccggc agccgggtga gctcgactgg gtccatgcac
     7861 tggccgtcga ccgtcgtggc aacctttacc tgggtgatat ccagggccgg cgtgcccaga
     7921 agtttatccg gctcgaaccc gccgttcgcg acggtgacgt cgccaaaaaa tcgctgccaa
     7981 ggcgagacga acccgtggag cgggcgggca agccttgatc aggcattcgg agacaggatg
     8041 ggaaccgcat cgcgggtcat cactgcactc aagctcgcgc cgaaagtctc tgctgtaccg
     8101 cgcatctctt ggtcacggtg atcacgccga gcttccggcc gaccgcgcgg caaccaaata
     8161 aaccgcgtgc gaccagatgg gcgccagtcg tcagacccgc aattccactc gcgggtttct
     8221 tcttcgacag gcgcgggacc acgacggctc cacgatcggg caccgagaca acgcgcttca
     8281 agtcggcgag ggcttctccc ttcccgacca cagtctgccc ctctccaaat gtcacacagg
     8341 tagaccaatt gtccatcgca ccaaaaatat gatcaagtgt atccataact cttttcctcc
     8401 tggaattcac cccgggatct gcacgatccg cactcgtctc gcgttcgaga cattggtagc
     8461 aaacgccgtg cctgactatt gagtcgatag gccattggaa ctttgtggca tcatctttgc
     8521 cgatcaagtt ttacgaaggt ctctctttct acgtgatgcg catccg
//
LOCUS       DATAQL010000137         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_777690, whole genome shotgun sequence.
ACCESSION   DATAQL010000137 DATAQL010000000
VERSION     DATAQL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777690"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1531)
                     /locus_tag="VHS97_02300"
     CDS             complement(113..1531)
                     /locus_tag="VHS97_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3447052.1"
                     /translation="MGSIAGLVAGAAVGGLVIWLASKRLLCDATACLAALGALEVDHT
                     PGTREMDADRPLPVLGSAAFDAWIAHLYDLLAHLRRVQSEYAGAEQLVRRLGDTINGS
                     RGSKERSVSLESGAEGAAQPLLVFLDQFRRSALQINRDVSALEEANERVASGAHDQSE
                     AVSRTATSVEALSDRIDRIARNAGEAADACESARQEAQQGLEQVHSVIEGMDRLLSRI
                     EANGRKVRRLDDRSTEIGVIVELIRGISSRTDMLALNATIESIRAGEHGRGFAIVAEE
                     IRKLAERAAVATREIGTLVEAIQADTQESIRALSEEQAQTQRESQRIRETGTSLARIR
                     QVVEKSARLVEGISRSTNDQVLTTQDLVRAMQRISDVTQQTQERTTQARGFISALNQS
                     CEPWQRLAAVDPVPDDSASRPAGAGLQLPEELPPLGAGLITPPSFGSSRTISSPSIRS
                     PHGSSKLRQSPGRKGVGSEHSR"
     gene            complement(1646..>2244)
                     /locus_tag="VHS97_02305"
     CDS             complement(1646..>2244)
                     /locus_tag="VHS97_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3447053.1"
                     /translation="PKQWFCLFRGDAGPMAISVESVAEVLETDSLIRLAWSPPQVVGM
                     CSYHREVVPVVKLGPLPREVGDEVPGRRDQTVAIDTAGEMAVIDESTRHVVLILKTEH
                     GAWGIGVDSENTIMSRESPECHSPRIYPNGLVLIGVIRLAGTCYGIVDAEATWLGLRS
                     AVSRWSGLISESNPSSPLPSGEEPRSVDPGAIGKYREA"
BASE COUNT          399 a          726 c          699 g          420 t
ORIGIN      
        1 gcccttggag tcaggtgttc ctggccctgc gacgcgtttg tccacaattc ggcgagtatc
       61 gccagacgat cggctagcga agtcgccgtc gcgggggctg gagatccaga cgtcatcgcg
      121 aatgctcgct cccgactccc ttgcgtccgg gactttgccg caacttcgaa gacccgtggg
      181 gggaccggat tgacggagag gagatggtac gggaactccc gaacgagggc ggggtgatga
      241 gacccgcgcc taatggtggc aactcttcgg gcagttggag acccgcgccc gcgggtcggc
      301 tcgcggagtc atccggaacg ggatcgacgg cggccaggcg ctgccagggc tcgcacgatt
      361 gattcagggc actgatgaat ccacgggcct gtgtggttcg ttcctgagtc tgctgcgtca
      421 cgtcagagat cctctgcatg gctcgcacga ggtcctgcgt ggtcaggacc tgatcgttgg
      481 tcgaacgcga gatcccctcg accagccggg ccgatttctc gaccacctgt ctgatccggg
      541 ccaaggatgt accggtctcg cgaatccgtt gcgactcgcg ctgcgtttgc gcctgttcct
      601 cgctcagagc ccggatgctt tcctgcgtgt cggcctggat ggcctcgacg agcgtgccga
      661 tctcgcgggt ggccacggcg gcgcgctcgg ccagcttgcg gatctcctcc gcgacgattg
      721 cgaagccacg accgtgctcg ccagcgcgga tcgattcaat cgtggcgttg agggcgagca
      781 tgtcggttcg gcttgagatc cctcggatca actcgacgat cactccgatc tcggtcgaac
      841 ggtcgtccag ccggcggacc tttcggccgt tggcctcgat ccgggagagg agccgatcca
      901 tcccttcaat gacgctgtgc acctgttcga gcccttgctg ggcctcttga cgcgcgcttt
      961 cgcaagcatc cgccgcctct ccggcattgc gggcgatgcg gtcgatccgg tcggacagcg
     1021 cctccacgct ggtggcggtc cgggagacgg cctccgactg gtcatgagcc ccagaggcga
     1081 cccgctcgtt cgcctcttcc aatgccgaga cgtcgcggtt gatctgcagc gcggatcgcc
     1141 gaaattggtc gaggaagacc agcagaggct gagccgcccc ttccgcgccc gattccaaac
     1201 tcactgagcg ctctttcgat ccgcgggacc cgtttatggt gtcgccgagt cttcgcacca
     1261 actgctccgc gccagcatac tcggactgga cgcgcctcag gtgggcaagg aggtcgtaca
     1321 ggtgagcgat ccacgcatcg aaggccgccg atcccaggac tggtagcggt cggtccgcgt
     1381 ccatctcgcg tgtcccgggg gtatgatcaa cctcaagagc tccaagggcc gcgaggcagg
     1441 cggttgcgtc gcatagtagt cgcttggagg ccagccagat caccagtccc cctacggccg
     1501 caccggccac gagaccggca atagagccca tagcagaccc caggcagagg actgatcccg
     1561 cccctatggc agcaccggcg ctaccggtga tcgcgccgca agtcaacatt cgtggatggc
     1621 aattcaagat cgacctcgac agtacttatg cctcgcgata tttccctatg gcgccggggt
     1681 cgactgatct cggctcctct ccggaaggca gcggagaaga agggttggat tcgctaatga
     1741 gtccggacca ccggctgacc gcggagcgca aaccaagcca ggttgcctcg gcgtcgacaa
     1801 tcccgtagca ggttccggcg agccgaatga ctccgatcaa cacaagtccg ttgggataaa
     1861 tcctgggcga atggcattcg ggagattccc ggctcatgat cgtgttctcg gagtcgacgc
     1921 caattcccca agcgccatgc tcggttttca ggatcaagac aacatgccga gttgactcat
     1981 cgataacggc catctctccg gccgtatcta tcgcgaccgt ctggtctcgc cggcccggga
     2041 cttcatcccc gacttcgcgc ggcagagggc ccaacttgac gactggaacc acctctcggt
     2101 ggtacgaaca catcccgact acttggggcg ggctccatgc caatcgaatc agcgagtcag
     2161 tctcgaggac ctcggccacg gattccacag aaatcgccat tggaccggca tctccgcgga
     2221 agaggcaaaa ccactgcttg ggcc
//
LOCUS       DATAQL010000138         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4900623, whole genome shotgun sequence.
ACCESSION   DATAQL010000138 DATAQL010000000
VERSION     DATAQL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4900623"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2987
                     /locus_tag="VHS97_02310"
     CDS             <1..>2987
                     /locus_tag="VHS97_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447054.1"
                     /translation="TVAGEPDARDDPARAGCERLQAVLLERRIERELARPAVGWSADG
                     LWAERFHLSRLQTQVESLDLSQVTAIRLNKPDSGSTGPESRLPAFATSPIEWLASLER
                     RRALLADDAFDRISALDLASRAESCERVVQTAADEVGETIAFLEDMPLRRAVTRLKLM
                     REDLERMAVSCRRAARKAKNGAESSSGGETHHESKSSHEFVADHLSDQLEPAAIKRLR
                     RQTGRVERLSRRVQSAWQEKLLALRMQRLFGHPFVAILENTVLFLILVLFALIAAQVV
                     LEQTSTTGLSVRQHEFFAWADLVICSVFLFEFGLKLVLAPNRLTYLARHFVIDLVASL
                     PFGFIFNQIAIAQLENAAIGAGPPSGPFRPFLRIGRIAIRFLRVALPILRLLRIPLIL
                     LRLSDRLVRRMAGLLNRNIVLFEPSQAQKAESSDRHRLVTLRSELEHARNAIEARLDR
                     EQRRQLAERILGDLECRIDGLPAEAIDEASEEAHGREIPVERVVEQLIQMTPERLVDR
                     AGPAFVRSFDRYLRLLDLPLLRRLPLVRNLVAYREKSPAEAGALAANYLGHLIQRGLD
                     VVYFFADLHGTLSPPVFLDRLGATLVNATRTPAKRLLWLGSAFLFLFVVVNALTFLKP
                     LRGIVDRLQTLMGWPVIILGGICLIFWYLGAWFRKIANQSADFCERVVEAQFAAHTKN
                     LKSRRRHQDAQFLAARVIDPELLLRASDDRLPPLVHSKKDEDPEQHGHDLFENRELAF
                     LRNVRLLYQDYLDGSPLHRSDTKASVQLLGNLALGNLRRSHLGHLLREGRSIDRLDLS
                     RAGGLFGGPYLWFNYITRMVVQETALLILDYNRHAIPVDRLACSPEAIRRAFQFWLAG
                     RLKIDPDEVWLPERATADSIGRDGLNSGCSPAAPPLDGATPLEQSPRREARMFLETVE
                     FTAVDFLADEAERDAEIHARFGPQVAELVRRDRQQNVRRAFRSFPLHELPLADRTINP
                     LTLYETYLAGGRVALLPFILARA"
BASE COUNT          499 a          961 c          906 g          621 t
ORIGIN      
        1 ccaccgttgc cggcgagccc gatgcgcgcg atgatccggc tcgtgccggc tgcgaacggt
       61 tgcaagcggt tctcttggag cggcgaatcg agcgcgagct agcccggccc gccgtcgggt
      121 ggagcgccga cgggctctgg gcggagcgat ttcatctctc gcggctccag acccaagtcg
      181 aatcgctcga cctcagtcaa gtcacggcca tccgcctgaa caagccagat tccggttcca
      241 ccggcccgga gtcgcggctg ccggcattcg ccactagccc gatcgagtgg ctggcctccc
      301 tggaacgccg ccgcgcccta ctagccgacg acgcattcga tcggatcagc gcacttgatc
      361 ttgccagccg cgccgaatcc tgtgagcgcg tcgttcagac ggctgccgac gaggtcggcg
      421 aaacgatcgc gtttctcgag gatatgccgc tccgccgcgc cgtcacgcgg ctgaaattga
      481 tgcgagaaga cttggagcgg atggccgtct cgtgccggcg cgcggcgcgc aaagcgaaaa
      541 atggggccga gagctcgagt gggggcgaaa cccatcatga gtcgaaatcg agccacgaat
      601 ttgttgccga tcatttgagc gaccaactcg agccggcggc gatcaagcgg ctgcgccgcc
      661 agacgggccg cgtcgagcgg ctgtcgcgac gggtgcagag cgcgtggcaa gaaaagctgc
      721 tggccctgcg gatgcagaga ctctttggcc acccttttgt cgccatactg gaaaacaccg
      781 ttctttttct gatcctggtt ctctttgcct tgatcgcggc ccaggttgtt ttggagcaga
      841 cctccacgac gggcctctcg gtccggcagc acgagttctt tgcctgggcc gatctggtga
      901 tttgttcggt cttccttttc gagtttgggc tcaagctggt ccttgccccc aatcgactga
      961 cgtatttagc ccgccacttc gtgatcgacc tggtggcgtc gctgccgttc ggcttcatct
     1021 tcaaccagat cgcaatcgcg caacttgaaa atgcggcgat tggcgccggt ccgccgtcag
     1081 ggccgttccg cccgttcctt cgcattggcc ggattgcgat tcgctttttg agggtggcgt
     1141 tgccaatcct caggttgttg cgcattccgc tcatcctctt gcggctctcc gatcgcctgg
     1201 tgcgccgcat ggcgggattg ctcaaccgca atatcgtgct gttcgaaccg tcgcaggctc
     1261 aaaaggccga gtcgagcgat cgccaccgcc tggttacact acgaagcgag ctagaacacg
     1321 cgagaaatgc gatcgaggcc cggctcgatc gcgagcagcg ccggcaactc gcagagcgta
     1381 tcttgggcga cctggagtgc cggatcgatg gcctgccggc cgaagccatc gacgaagcgt
     1441 ctgaagaggc ccacgggcgc gagattcccg tcgagcgggt tgtcgagcaa ttgatccaga
     1501 tgacgcccga acggcttgtc gaccgcgcgg gaccggcgtt tgtgcggagc ttcgaccgct
     1561 atctccggct gctggatctg ccgctcttac gccgtctgcc gctagtgcgc aatctggttg
     1621 cctaccgcga aaaaagcccg gcggaagcgg gtgcgctggc cgccaattat ctgggccacc
     1681 tgatccagcg cggtctggat gtcgtctact ttttcgccga cctgcacggc acgctttcgc
     1741 cgccggtttt cctcgaccgc ctgggggcca ctctcgtcaa cgccacgcga acgcccgcca
     1801 agcgtctctt atggctaggt tccgcgtttc tctttttgtt cgtggtcgtc aacgccctga
     1861 cttttttgaa accgctcagg ggaattgtcg acaggctcca gaccttgatg ggctggccgg
     1921 tgatcatcct gggaggcatc tgcctaatct tctggtacct gggtgcgtgg ttccgcaaaa
     1981 tcgccaatca gtccgccgat ttctgcgagc gggtggtcga ggcccagttt gcggcccaca
     2041 ccaagaactt gaaatcccgc cgtcgtcacc aggacgccca gttcttggcc gcacgggtca
     2101 tcgaccccga gctcttactc cgcgcttccg atgatcggct ccccccgttg gttcactcca
     2161 aaaaggacga ggatcccgag caacatgggc acgatttgtt cgagaatcgc gagcttgcgt
     2221 tcctgcgcaa cgtccgtttg ctctatcaag attacctcga tggatctccc ctgcaccgca
     2281 gcgatacgaa ggcgtcggtc cagcttttgg gcaacctggc tctgggcaat ctgaggcgca
     2341 gtcacctggg ccacttgctc agggaaggcc ggtcgatcga tcggctcgat ctgagccgtg
     2401 ccggtggtct gttcggcggc ccgtatctct ggttcaacta catcacccga atggtcgttc
     2461 aagagacggc gcttttaatt ctcgactaca atcgccacgc gattccggtc gaccggctgg
     2521 cctgctcgcc cgaggcgatt cgtcgcgcat ttcaattctg gcttgctggc cggctcaaga
     2581 ttgatccgga tgaggtctgg ctgcccgagc gagcgaccgc cgactcaata ggccgcgacg
     2641 gccttaattc gggctgttcg ccagcggctc cgccgctcga cggtgccacg ccactcgaac
     2701 aatcaccccg ccgtgaggcc cggatgttcc tggagacggt cgagttcaca gccgtcgact
     2761 tcctcgctga cgaagccgag cgcgacgccg agatccacgc tcggtttggt ccccaggttg
     2821 ccgagctagt gcgacgagac cgtcagcaaa atgtgcgccg ggcatttcgc agcttcccgc
     2881 tgcacgagct gccactggcc gaccggacga tcaacccgct tacgctttac gaaacgtatc
     2941 tggcgggggg ccgtgtcgcg ctgttgccct ttatcctggc gcgagca
//
LOCUS       DATAQL010000139         6385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2152537, whole genome shotgun sequence.
ACCESSION   DATAQL010000139 DATAQL010000000
VERSION     DATAQL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152537"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VHS97_02315"
     CDS             <1..940
                     /locus_tag="VHS97_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447055.1"
                     /translation="HERGTYMMHTGYAPNPTVVHPGWGSVCAMELGEKLENFDLPHCI
                     SINSPGVGAGFLGMSYAPFVVQNPNAPIANLRPPQDVDGLRMDRRFELLGLVENQFVA
                     QRRAQAAVDHKAVYAKTLRMMNSRYQDSFNLDRESAKVRDAYGRGSFGSGCLMARRLV
                     EKGVSYVEVSLGGWDTHNNNFDTLSNRLLPELDKGMSALVDDLADRRLLDTTTIVWMG
                     EFGRTPKINQNAGRDHHPGSWSVVVGGGGLKGGQVIGATDADGMDIIDHPIGVMDLIA
                     TMTKTMGINVETQYTTPRGRPIKVVDGGKPIKELIG"
     gene            complement(1151..6343)
                     /locus_tag="VHS97_02320"
     CDS             complement(1151..6343)
                     /locus_tag="VHS97_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447056.1"
                     /translation="MRKSWRFQDEAGAILSTQAISESDRAALGFPNDGLAIEVRVTVP
                     RHAEKAVTFHAEYSWNNQGPIPLVSLGRAYLAQGLVICETPASLRSRATTTLLCRLDG
                     ATLEQADFETGEEYATGSRDDRGLSRGTTVEAFSYTEPGSRLELFAEPLEPSGITGIV
                     REAILSTLVDHEGKLLNRLRLVVNLGEARSLEFVLPQEISLVRVRRDGTDVAPIAKPT
                     GFSIPVTGAAQGTRSSTIVFDYVTPRRTFADGERLRPDLPRVDLPCLSFVWEVITSPA
                     WRADDAGPGLVAGDRNDFREWPYAALGVPTPLWTIFMPGRGRQQNESVLRLLDEKLAD
                     RAVTELSFAEWFSRWDSGPWPVVIDRVALSTAGLGPKSQCVLPTVKTEGRNLALATLK
                     QHGLALAPVQNVLVITTEAELSRLESADRGAEQIREALVWGSDRTDRFQSVARWRGEA
                     SPRIASVIGEEPAERVKLPPGWSARRFAGSSWPASDSFVRLANVQKRIVMGWIIAATC
                     ILAWVSYRPRLVFFRYPLLTATLALCLFCEWLLPASYASYSAAVYVAAFSILIVELGR
                     DCVRSVGPGRAARRPDSALGRRAVGATVSAALAALLMDNVAPIQAAAPADRESTILAL
                     YPYDGPFDPSRSVTDVIIRLADFNRLKLMAETEVPQVNSSVRAIGAVHRVTWKSDLEV
                     IVESEIELVAAGRAPFVWKLPVSQARDIQTVVDGKRTPVAIEPGGAVGKVEILEAGKH
                     VLRVRRSSAPANDYGQSHLVLPVNAIPSARVLVQPREGGRQDAELTAFGRTKTQLDGS
                     LTALLGPADRILVRWGKPAGASVVRALGVVDGLILWDITPAGDRVRARFTTHQPRPLS
                     TFTFGHQEGMILRSAQVAGSGETVWEENSARREWTLHVDPPLQSGSTIALECWLPHDA
                     ARDDKGGRSRAEAGLPPEPSRSLPGLEPIGMERYAGSLGVRRPGGWTGRFDPLPETDP
                     ISDELFVQAWGALPEEPLTLCGTSRFVGECGASLQTGPPPTRIQVKPTIQLQIESGRI
                     ALTVDAELTELAGHLRDAEAVIPEDLQITSVTAEGLSDWTVSTDHRLHMKFDRSISRP
                     KRLLRVLGWIALSEDPTQLSGRQHRQKTPWFWWDGLEPSVGFLTISSIAKPEMRESTG
                     LTRISSESSGGVGATTPRHSSTYRVDDPRKLGEILWESVPPGVRVSIESQITIHPDSA
                     EWVAVLRYDVIGGALDAIYLKMPAAWSTGAALHFPSGKYQLTTETRGADAFWTITPER
                     PIWGSQRFVLRSSRHLESDRPIVHPEIAPRGKGVVDAYLSIINATGQPLAPANSVGLQ
                     SIPYASRFQAREFATFGGTAVGAFRVVKDPWTVSVELPKIASRDMDSWSGSAQVAFAE
                     TMMVVMPDRSSLGRAVYETVPGTGPNLSFELPPGSTLVWATVDFNPAVPLRSSSGIWS
                     ILCDNRRQSRIGLIWRTEPTTSRSMRSTSSVGLPRAGAGSANTLVSIYTPPGLTVSRG
                     DSSGLEIAGMARLEMARADWLARCVGDLVAKFDRSSGRDHEKLVALLINHEMALRGAL
                     RNIQALDPARAKVESDRFANDFATLRSARAARNETIVRAGLEEDVAAAQTYLGEGSPT
                     LARPHFGAPEPIASDRIRFLGRPTTLIGLIPGVDVTAPPTILTLETRPWDDLASTLPL
                     QAIITIVLISGIALATVGMGRWNWQGSVALLAALGLAAYTGGPTLLATGLALALVGWK
                     TARG"
BASE COUNT         1299 a         2011 c         1928 g         1147 t
ORIGIN      
        1 ccacgagcgc ggcacctaca tgatgcacac cggctatgcc cccaacccca cggttgtcca
       61 ccccggatgg ggctcggtgt gcgccatgga actgggcgaa aagctcgaga atttcgatct
      121 gccccattgc atctcgatca attcaccggg cgtcggcgca ggattcctcg gtatgtcgta
      181 cgcaccgttt gtcgtccaga atccgaacgc accgatcgcc aacctgcggc ccccccaaga
      241 cgtcgacggc ttacggatgg atcgacggtt cgaattgctc gggcttgtcg aaaaccagtt
      301 tgttgcccaa cgacgcgcac aagcggctgt cgatcacaag gcggtctatg ccaagacgct
      361 ccgcatgatg aattcgcgtt atcaagactc gttcaacctc gaccgcgaat cggccaaggt
      421 ccgcgacgcc tacggccgtg gctctttcgg ttcgggctgt ttgatggcca ggcgcctggt
      481 cgaaaaggga gtcagctacg tggaagtatc cctgggaggc tgggatactc ataacaacaa
      541 tttcgataca ctctccaatc ggctgcttcc tgagctcgac aagggaatgt cggcattggt
      601 cgacgacttg gccgatcggc gccttctcga taccaccacg atcgtctgga tgggggagtt
      661 tggccgcacc ccgaagatca accaaaacgc gggccgcgat catcacccag gtagctggtc
      721 ggtcgtggtt ggcggcggag gattgaaggg cggccaggtc attggcgcga cggacgccga
      781 tggcatggac atcatcgacc accccatcgg cgtgatggac ttgattgcca ccatgaccaa
      841 aaccatgggt atcaacgttg aaacccagta cacgacgccg cgcggccgtc cgatcaaggt
      901 cgtcgacggc ggtaagccga tcaaagagct gatcggttga taggttgtac tctactagat
      961 gcggcgaatg gattcactcg acggaagacg acgcctgctc atgagagccg gcgtcgtgcc
     1021 gtcgcacgcc agacttcaat tccattcgta ggttgggcac cgctccattc gtaggttggg
     1081 caccgcccac cagtctactt tcggtgggcg gtgcccaccc tacaaatcgc gaaagcggtc
     1141 gtccaatcgg tcagcctcga gccgtcttcc agccgacgag cgccagcgcc agcccggtcg
     1201 ccagcagcgt cggtccacct gtgtaggcag ctagcccgag tgcggcgagc agcgcaacgg
     1261 aaccctgcca gttccagcgg cccattccta ccgtggcgag cgcgatgccg ctgatcagca
     1321 caattgtgat gatcgcctga aggggcaagg tacttgctaa gtcatcccac ggtcgagtct
     1381 ccagcgtcaa gatggtgggc ggagcggtca cgtcgacgcc cgggatgagt ccgatcagcg
     1441 tggtaggtcg gcccagaaag cggatacgat ccgaggcgat cggttcgggt gcaccgaagt
     1501 gcggacgagc cagagtagga gacccttctc ccaaataagt ctgcgcggca gcgacatctt
     1561 cctcgagtcc ggctctcacg atcgtctcgt ttcgggcagc gcgggccgat cgtagcgtag
     1621 cgaagtcgtt ggcaaatcgg tcactttcca ctttagctcg agccggatca agtgcttgaa
     1681 tgtttctaag agcgccgcgc agggccatct cgtgattgat gagcaacgcc accagctttt
     1741 catggtcgcg ccccgagctg cgatcgaact tggcaacgag atcgccaaca cagcgggcga
     1801 gccagtcggc ccgcgccatc tccaaccgag ccatgccggc gatctcgaga ccgcttgaat
     1861 cgccccggct caccgtcaac cccggcggcg tgtagatgga aaccaacgtg tttgccgatc
     1921 ccgcccccgc gcgcggaagt cctacggacg aagtggagcg cattgaccgt gacgtggtcg
     1981 gctcggttct ccagatcaag ccgatgcgcg attggcgccg attgtcgcag agaatagacc
     2041 agatcccgga tgatgagcgg agcggaacgg ccggattgaa gtcgaccgtt gcccagacga
     2101 gagtgctgcc aggtgggagc tcgaacgaca ggttcgggcc tgtaccagga accgtctcgt
     2161 agacggccct gccgagactc gaccgatccg gcatgacaac catcatcgtt tcggcaaagg
     2221 cgacctgggc cgaaccgctc cacgaatcca tgtctcgcga ggcgatcttg ggcagctcaa
     2281 cactgaccgt ccacggatcc ttgacgaccc gaaacgcgcc gacggccgtc ccaccgaaag
     2341 tggcaaattc tctggcctga aaccttgagg catacggtat cgactgcaag ccgacggagt
     2401 ttgcgggggc gagtggttgc cccgttgcgt tgatgatgct cagataagca tccacaactc
     2461 ccttaccgcg cggagcgatc tcagggtgaa cgatcggtct atccgactcc aggtgacggc
     2521 tcgaccgaag cacaaaccgc tgagaacccc agatggggcg ctctggtgta atcgtccaga
     2581 aggcatccgc gccccgcgtc tcggtcgtca actgatactt gccgctcgga aaatgcagag
     2641 ccgcgccggt agaccaggcg gcgggcatct tgaggtagat cgcgtcgagc gcgcctccga
     2701 tcacatcgta acggagaacg gccacccatt cggccgaatc gggatgaatg gtaatctggc
     2761 tttcgatcga gactctgaca ccaggaggaa ccgattccca gaggatctca ccaagtttgc
     2821 gaggatcatc gactcgatag gtcgagctgt gtcggggcgt cgtcgctccc actccgccgg
     2881 acgactctga agaaatcctg gtcaagccgg tcgattcgcg catctccggc ttggcgattg
     2941 atgagattgt caaaaagccg acgctcggtt ccaggccgtc ccaccagaac catggcgtct
     3001 tctggcggtg ctggcgaccg ctgagctgcg tcggatcttc gctgagcgcg atccacccga
     3061 gcacgcgcaa gagtcgtttc ggccggctga tcgaacggtc aaacttcatg tgcaagcgat
     3121 gatcggtgga aaccgtccag tccgacagtc cttcggcggt cacgctcgtg atctggaggt
     3181 cctcgggaat cacggcctcg gcatcacgaa gatggccggc cagctcggtg agttcggcat
     3241 cgacggtcag ggcgattcgc ccggattcga tctgaagttg aatcgtgggc ttgacctgaa
     3301 ttctggtcgg aggcgggcct gtttgtagcg acgcgccaca ctcaccgacg aaccgactgg
     3361 tcccgcaaag cgtgagcggc tcttcgggaa gtgctcccca ggcttgcaca aacaactcat
     3421 cactgatcgg atcggtctcg ggcaacggat caaagcggcc cgtccaacct cctggccgcc
     3481 gaacgccaag cgatcctgcg tatctttcca tgccgatcgg ctcgagaccc ggcaggctgc
     3541 ggctcggttc gggcggaagg ccggcctcgg cccgcgaccg tcctcccttg tcgtctcgtg
     3601 ccgcgtcatg aggcaaccag cattcaagcg cgatcgtcga gcccgattgt aaagggggat
     3661 ccacatgcaa ggtccattca cgcctggcag agttttcctc ccagaccgtc tcgcccgagc
     3721 cggcgacttg cgccgatctg agaatcattc cttcctggtg cccgaaggtg aaagtggaaa
     3781 gcggtcgcgg ctgatgagtc gtgaaccgcg cacggacgcg gtcgccggcc ggagtgatgt
     3841 cccacaagat caagccatcg acgacaccta aagcgcgcac gaccgacgcc ccggcgggct
     3901 tcccccaacg aacaagaatc cggtcggcgg ggcctaagag ggcagtgagc gaaccgtcga
     3961 gctgcgtctt cgtccggcca aatgcggtca attcggcatc ttgacggcct ccttctcgcg
     4021 gttgcacaag gacgcgagcg gacggtatcg cattcacggg aagaacgaga tgagactgcc
     4081 cataatcgtt tgcaggcgcc gacgaccgcc gaacccggag gacgtgtttg cccgcttcca
     4141 gtatctcaac tttgccgact gcgcctcccg gctcgattgc aaccggcgtc cgcttcccat
     4201 ccacaactgt ctgaatgtcg cgcgcctggg aaaccggcag tttccagaca aagggcgctc
     4261 gacccgcggc taccaactcg atctcactct ccacgatgac ctcgagatcg ctcttccatg
     4321 tgacgcgatg aactgccccg atcgcgcgca ccgaggaatt cacctgtggg acttcggtct
     4381 cggccataag cttgagccgg ttgaaatccg cgaggcggat aatgacgtcg gtgaccgagc
     4441 ggctgggatc gaaggggccg tcatacggat aaagcgccag tatcgttgat tcacgatcgg
     4501 cgggcgcggc ggcttgaatg ggggcaacgt tatccatcaa caaggcggcg agcgccgcgc
     4561 tcacagtcgc gccaacggcg cggcgtccca aggcgctatc cggacgcctc gccgcgcggc
     4621 ctggcccaac cgatcgtacg caatcgcggc cgagctcgac gatcaagatc gaaaatgcgg
     4681 cgacataaac cgcggcgcta tagctcgcat aacttgcggg aagcagccat tcacagaata
     4741 ggcaaagagc caaggtcgcg gtcagtaggg gataccgaaa gaagacgagc cgagggcggt
     4801 aagacaccca ggccaaaatg caggtcgcgg cgatgatcca tcccatgaca atgcgcttct
     4861 gtacgttggc caaacgcaca aacgaatcgc tggcgggcca ggacgaccca gcaaaccgcc
     4921 gggccgacca tcccggtggt agcttgaccc gctcggccgg ctcttcaccg atcaccgagg
     4981 caatcctcgg cgaggcctca ccgcgccagc gtgccacgga ctgaaatcga tctgtccgat
     5041 cggaacccca aaccaatgct tcgcgaatct gctcagcccc gcggtcggcc gactcgagtc
     5101 ggcttagctc ggcttcggtt gtgatcacga gaacgttttg aactggtgcg agcgctaacc
     5161 cgtgctgctt gagcgtcgcc aacgccaggt tgcgcccttc tgtcttgacg gtgggaagaa
     5221 cgcattgcga tttcggtcct agtcctgcgg tgctcagggc aacgcgatcg ataacgaccg
     5281 gccatggtcc cgagtcccag cggctgaacc actccgcgaa actcagctct gtcaccgccc
     5341 tatcggcgag cttttcgtcg aggaggcgca gcaccgactc attctgctgg cgcccgcggc
     5401 cgggcatgaa gatcgtccac aacggtgtgg gcaccccgag cgccgcgtat ggccattccc
     5461 ggaagtcgtt gcgatcgcca gcgacgagac ccggcccggc gtcgtcagcc cgccaggcag
     5521 gcgatgtgat gacctcccac acgaaagaca agcaggggag atccacacgc ggaaggtctg
     5581 gcctcaagcg ctcgccgtcg gcgaaagtcc gccgcggcgt cacgtagtcg aagacgatcg
     5641 tgcttgacct cgttccctgg gccgcgccgg tcacgggaat cgagaaacct gtcggtttcg
     5701 cgatcggcgc cacgtctgtt ccatcacgtc gcactcgaac gagcgagatc tcttgaggca
     5761 agacaaattc gagcgagcgg gcttccccaa gattcacaac aagacgaagt cggttcagaa
     5821 gctttccctc gtgatcgacc aaggtcgaca agatagcctc gcggacgatc ccggttattc
     5881 cggatggctc cagcggctcg gcgaagagct cgaggcggct gcccggctca gtgtatgaaa
     5941 atgcttcgac cgtcgttcct cttgagagcc cccggtcgtc gcggctcccc gtcgcgtact
     6001 cctcgccagt ctcgaaatcc gcctgttcca gggtcgcgcc gtcgaggcgg cacaaaagcg
     6061 ttgtcgtggc gcgagaccgc aacgaggccg gcgtttcgca gatgaccagg ccttgtgcga
     6121 gatacgctcg tcccagggaa accagcggaa tcgggccttg gttattccac gaatattccg
     6181 catgaaaggt caccgccttt tcggcgtgcc tgggtaccgt gacgcggact tcaatggcga
     6241 gcccatcgtt gggaaaaccc agcgctgcgc ggtcggactc acttatggcc tgcgtcgaca
     6301 gaatggcgcc cgcttcgtct tgaaaccgcc aagatttccg caatccaccg gcctggccaa
     6361 tccagagagg aaccgagtca atcgg
//
LOCUS       DATAQL010000140         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5053669, whole genome shotgun sequence.
ACCESSION   DATAQL010000140 DATAQL010000000
VERSION     DATAQL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5053669"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VHS97_02325"
     CDS             <1..1008
                     /locus_tag="VHS97_02325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3447057.1"
                     /translation="ALFLIIGAALAIYGTYVLNGLRCDLHEAKKFGQYQLRQKLGVGG
                     MGEVYLAEHQMLKRPCALKLIKPEAGADPIALARFEREVQSAARLSHPNTIEIYDYGH
                     TDDGTFYYVMEYLPGMSLQDLVQTYGPLPARRIIYFFRQVCAGLAEAHAIGLVHRDLK
                     PANIYITVRGGEVDFAKILDFGLVKLTKDKGAVALTSDQRVSGTPLYMAPEQVMGDRS
                     LGARADIYALGGMMYFTLTGQPPFKGGSPFEVMMAHARDPVVPPSQLRPEVPADLEQV
                     VLRCLAKKPDDRYPDVKALGKALAACAAATEWDAEKAEEWWSEVSRSSTADAGVALAQ
                     T"
     gene            complement(1149..1508)
                     /locus_tag="VHS97_02330"
     CDS             complement(1149..1508)
                     /locus_tag="VHS97_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447058.1"
                     /translation="MSENISGEGSSSDPMKTVADALEKAVQTATDSAADAKASLEKSL
                     PAASRFVSRLVYTTCYSLSYGVVFPTVWVARSIPPDNPIVTGLAEGAMAAIDAVDQLK
                     NRQLAAPVVESSQPQPS"
     gene            2025..2579
                     /locus_tag="VHS97_02335"
     CDS             2025..2579
                     /locus_tag="VHS97_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447059.1"
                     /translation="MRRKRTFLQGTLDLLESRVVLSRTALGTPVVVSGLYPHQRILNR
                     QQQPVAAEINQVFDSFQNDYDQARATYFTSIQNQANPSLATTNAFVFYTTQRVSLIGQ
                     QTLNIFVQTKHTPRQTQSLERLVATKIIGAQAQTPPGSLAQSLLQTIPQPGTSAPTSS
                     LYSLSEDNAIEAARVAILNGVNNL"
     gene            2849..4249
                     /locus_tag="VHS97_02340"
     CDS             2849..4249
                     /locus_tag="VHS97_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447060.1"
                     /translation="MLTLVVNPITAVAGQSVNSSVATFAAGDVQGTIANFQATIFWTG
                     AVNLTTTGFIAPSGPGTYTIYSSNVYAKPGSYAVNVVLTGANSSSAQASGTATVSDAP
                     LSTSPVSIDTLIQTPFSGEVAFFRTNNSYATSADFKASIDWGDSTPPTSGSISSNGFG
                     SFSIVGQHTYAIVGTLPITVTIVSPGGQTNIVNSTAIVTAPSVSVFPTNVIGNAGQPL
                     SGVTVATFLDPTISDTATDFRATVDWGDGTINVGSIVNQGDGVFSVTGNHTYTASGTY
                     TINIQVVRVADGQTASTSSTAQIGSPSPTFAFTGGLASVPANGPFFSSGYATTNQPTF
                     NGTAAAYAIVQLFARPLNVDAEVPLGQTVADSSGNWSLATGPLAAGGYTITAKVTPPA
                     GYPSLMMALTSNNGTFFIDLTPAGKVANSQTPAVAAETNRPVERSKTRNITLHRTPKI
                     PRERVARSAPRDGSIR"
BASE COUNT          968 a         1354 c         1249 g          868 t
ORIGIN      
        1 gccttgttcc tcatcatcgg ggcggccctg gcgatttatg gcacgtacgt cttgaatgga
       61 ctgcgatgtg atctgcacga agccaaaaag ttcggccagt atcaactgcg ccagaagctg
      121 ggagtcgggg ggatgggcga agtctacttg gccgagcacc agatgttgaa gcggccctgc
      181 gcgctcaaac tgatcaagcc cgaagcggga gccgacccga tcgcgctcgc acgattcgag
      241 cgcgaggtcc agtcggccgc gcggctttct catcccaaca cgatcgagat ctacgactac
      301 ggccacaccg acgatgggac cttttattat gtgatggaat acctgccggg gatgagccta
      361 caggacctcg tgcagacgta cggcccgctg cccgcaagac ggatcattta cttcttccgc
      421 caggtctgtg ccggactggc cgaggcacat gccatcggcc tggtacatcg cgatttgaag
      481 ccggcgaata tctatatcac agttcgaggg ggtgaggtcg acttcgccaa gatcctcgac
      541 ttcggcctgg taaaactcac gaaggacaag ggcgccgtcg cgctcacgag cgaccaaagg
      601 gtgagcggta cgccgctgta catggctcct gagcaggtaa tgggggaccg ctcgcttggg
      661 gcccgagccg atatctacgc cctgggcggc atgatgtatt tcaccttgac aggccagccg
      721 ccgttcaaag ggggttcgcc attcgaggtg atgatggccc acgcccgcga tcctgtcgtc
      781 cctccgtcac agctccgtcc cgaggtcccc gccgacctcg agcaagttgt cctgcgatgc
      841 ctggccaaga aaccagacga tcgctatccg gacgtcaaag ctcttggcaa agcactggcc
      901 gcctgcgcgg cggcaaccga atgggatgcc gagaaagccg aggaatggtg gtccgaggtc
      961 agccggtcga gcacggccga tgccggggtc gcattggccc agacttaacc cctccaatgg
     1021 gacggtcggc gcagtgcggt tgatcgaaag gtcagcaggc tgaaatcagt tggtaggcgc
     1081 agctacccgg tcgaccacca ctcacgttgc tttcccgttc aaagacggcg aatcaactag
     1141 ccaaattttc acgacggctg cggctgactc gactcgacta ccggcgcagc cagttgccga
     1201 ttcttcaact ggtcaaccgc atcgatcgcc gccattgccc cttccgccag accggtaacg
     1261 atcgggttgt ccggcgggat tgacctcgcc acccagacgg tcggaaacac cacgccataa
     1321 gagagcgagt aacacgtggt gtagaccaac cgagacacaa agcggctggc cgcgggaagt
     1381 gacttctcca ggctcgcctt cgcgtccgcg gcgctgtcag tcgcggtctg aacggctttc
     1441 tcgagcgcgt ccgccacagt cttcatggga tcactcgagg atccttcacc actgatgttc
     1501 tctgacatga acagggcctc tcccgaaaag cgattgtcaa ctcgatccga aacgacagga
     1561 ctcgctgagc cgcgccagag cgatcatacg ggcgaaatcg aaaaactgcg tgtggcgaga
     1621 tgggaccgaa caacggctaa ccgattgaca acatagtcga tccgccaacg ccgggagaac
     1681 agcgccgcag gtacaaaaat tcaatccgtt tgatgttaaa ctgggaagtc tgggacgtca
     1741 agcgccaaga tgccgaagaa ttccggttca agtcgcggtc aactgcaatc atgcgagagt
     1801 cattggtgcc tttcgtcgtc cgaccgacaa aagaacacgc tggtccgggg agttctcagc
     1861 cggtctcgct acatacgccc gcttaatgac ggaaaaatct catgtcggcg tttatgtgat
     1921 aaattcactc atagaccaag ctgttcgata aaagctcggg cagcgaatgc gtcttccggt
     1981 tgtagagatt cccatactcg ggatcacaag gaggcgatcc ggccatgaga cggaaacgca
     2041 catttcttca gggcacgtta gacctcttgg agagtcgagt cgttctgagc cgcacggcgc
     2101 tgggaacacc ggtggtcgta agtgggttgt acccgcacca gaggattttg aatcgccagc
     2161 aacaaccggt cgctgctgaa atcaaccagg tgttcgactc gtttcagaat gactacgacc
     2221 aagcccgcgc aacctatttt acttcgattc agaaccaggc gaatcccagc ctggcgacga
     2281 ccaacgcctt tgtgttctac acaacgcagc gggtctccct gattgggcaa cagaccctta
     2341 atatcttcgt gcagacgaag catacgccca ggcagacaca aagtcttgag cggctcgtgg
     2401 cgacgaagat catcggggcc caagcccaaa cgcccccggg ctcgctcgca caatccttgc
     2461 ttcagacgat cccgcaacca ggcacgagcg cgccgacttc ttcgctctac tcattgagtg
     2521 aagataacgc catcgaggcc gcgcgggtcg cgatcttgaa cggtgtgaat aacctttaga
     2581 atgggacatt cggcagttcg atcgttctcc gccccggaaa ccacgttcag cggcgaccgc
     2641 cgttcaggcg tgtctggcaa tgagccaggt gaaacggcgt tgttcccgac ggagcaatca
     2701 tccagttgtt gcgttccaga aaaactcggc ttgtctgaag cgaggcatga cgaccatgaa
     2761 cttttttggg tggacagcca gtccgatacg cggttcgcgc cagagacgtc cggtcgctgc
     2821 atgggttgaa cggctcgagc gaaggagctt gctgacgttg gtggtcaatc cgatcacggc
     2881 agtcgcgggt cagtccgtca acagctcagt tgctaccttt gcggcaggcg acgtgcaggg
     2941 gacgattgcc aacttccaag cgaccatctt ctggaccggc gccgtcaatc tcaccacgac
     3001 cggctttatc gcaccgagtg gaccggggac ctataccatc tatagctcga acgtgtacgc
     3061 gaagccggga tcctacgctg ttaacgtcgt gttaacaggt gccaacagtt cctcggcgca
     3121 agcctccggg acagcgaccg tctccgatgc gcccttgagt acgagcccgg tgtcaatcga
     3181 taccttgatt cagacgccgt tctctggcga ggtcgccttt ttccggacta acaactcgta
     3241 cgccacctcg gccgatttca aggcctccat cgactggggc gactcgacac cgcccacgtc
     3301 ggggtcgatc tcctcgaacg gcttcggttc cttcagcatc gttggtcaac atacttatgc
     3361 gatcgttggg actttaccga tcactgtgac gatcgtgtca ccaggcggtc aaaccaacat
     3421 cgtcaactca acggctatcg tgacggcgcc gtctgtttcg gtattcccga ccaatgtgat
     3481 cgggaacgcc gggcaacccc ttagcggcgt tacagtcgcc acgtttctcg atcccacaat
     3541 cagtgacact gccaccgact tccgggccac cgtcgactgg ggcgatggaa cgatcaatgt
     3601 agggtcgatc gtcaatcaag gcgatggcgt cttcagtgtc accggtaatc atacttacac
     3661 ggcatccggt acctatacta ttaacataca ggtcgttcgc gttgctgatg gccagacggc
     3721 gagcaccagc agcactgccc agatcggcag cccgagcccc accttcgcct ttaccggagg
     3781 cctggcaagc gtgcccgcta acggtccctt cttttccagc ggatatgcca ccaccaatca
     3841 gccgacgttc aacgggaccg ccgcggccta tgcgattgtg caactgtttg cccgaccctt
     3901 gaacgtcgac gcggaagtgc cactagggca gacggtggcc gactcaagcg gcaactggag
     3961 cttggcaacc ggcccgctcg ccgcgggtgg ctacaccatc accgccaagg tcactccgcc
     4021 ggccggttac cccagcctga tgatggcact gaccagcaac aatggcacct ttttcatcga
     4081 cctcacgccg gcgggcaaag tcgcgaattc tcagaccccg gctgtggcgg ctgaaacaaa
     4141 tcggccagtc gagcgatcga agacgcgaaa catcactctc caccgaacgc cgaaaatacc
     4201 tcgggaacgg gtcgcgcggt cggctccgag agacggctca attcgatgat ctcgcggaaa
     4261 gaaatctgtt aacacatcct ctttgccgac tgcgagcggg cctccaactg cctcggatcg
     4321 ctgagatctg gccccaaagt cgctttaggt tattggccga tcagcttcaa cgtgcaggca
     4381 gttttcctcg acgctgcaaa caagtcttcg caagattggt gcccgtagga aaagctcgc
//
LOCUS       DATAQL010000141         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3832786, whole genome shotgun sequence.
ACCESSION   DATAQL010000141 DATAQL010000000
VERSION     DATAQL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3832786"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VHS97_02345"
     CDS             <1..1203
                     /locus_tag="VHS97_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3447061.1"
                     /translation="NWGRTVGGSHLYVGAAAEALSRQHDVTVLHQFDDLSNAYMAEFL
                     DLDLSRVEFRLIPRLANAEAGAATRNPLARHQSAVRKHADVSAEFDLFVGNFSFQVPA
                     FNHSPRGVLVVDFPFKDYDWWHDFGQSPPRWTSPRGALRRLYRRYIWGERFASYHRDL
                     VNSEFTRGWMKRRWFIDSTVVYPPCRDAIAPAEKRPIILAVGRFVPDKKADVLVEAFR
                     TLCDQGLRGWELVLAGGLPGSEWEGADSYLENLKQRAQGYPIDLRMDVPGPDLTRLFE
                     TAAIFWHAKGYQVDAEVHPEHMEHFGIVTVEAMIAGCVPIVFAGGGQPEIVNDGADGV
                     LWRTQEELADRTRDLISNPDRLRELSGRAVGRGNTFSKDAFVSRFINAVSDLLPGQRD
                     RLALQSKS"
     gene            1454..2299
                     /locus_tag="VHS97_02350"
     CDS             1454..2299
                     /locus_tag="VHS97_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3447062.1"
                     /translation="MATTASPSNFQHIQIRSDSNLATLDLQEIFRYRDLLLTLADRDI
                     RVRYKQTVIGVAWVVLQPLMTSLIFAFVFGVLARLPSEGRPYILFAFSGLLAWNAFSQ
                     TLTRVSFALVGNVHLVSKVYFPRLILPLAAMASTLVDFVVALVLMFLMLIFYGVWPGW
                     GLLLLPVWLAVMLVMALGLGLVAAALMVKYRDISHIIPTILQLGMYVTPVAWSALIVP
                     GKFRWIFLANPLTGLLNAFRWSLLGEGALSLPALAYSTVTAALFFWCGAIVFKQQERN
                     FADVI"
     gene            2289..>2946
                     /locus_tag="VHS97_02355"
     CDS             2289..>2946
                     /locus_tag="VHS97_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3447063.1"
                     /translation="MSSNSIAISVKGLGKKYTIAHKSTRPTTLREAIVERLRHPFGYD
                     REEHESFWALRDVDFEIREGEVVGFVGRNGAGKSTLLKLLSRITGPTTGTIDIHGRIA
                     SLLEVGTGFHPELTGRENIYLNGAVLGMSRREITSKFDEIVAFAEVEQFLDTPVKRYS
                     SGMYVRLAFAVAAHLEPEIMIVDEVLSVGDASFQKKCMGKMQDFSGHGRTILFVSHNM
                     N"
BASE COUNT          518 a          873 c          929 g          626 t
ORIGIN      
        1 aattggggaa ggaccgttgg agggagccat ttgtatgtgg gagccgcggc cgaggccctg
       61 tctcgacagc acgacgtcac cgttctccat cagttcgacg acctatcgaa tgcgtacatg
      121 gccgaattcc tcgacctcga cctttcccgg gtcgagttcc gcctgatccc ccgacttgcg
      181 aatgcggaag cgggcgcggc gaccaggaac ccgctggccc gccaccaatc ggccgtcaga
      241 aagcatgccg atgtcagcgc tgaattcgat ctgttcgttg gcaatttctc cttccaggtg
      301 cccgccttta atcattcgcc acgcggcgtc ctggtcgtgg acttcccgtt caaggactac
      361 gactggtggc acgacttcgg gcagagcccc ccccgctgga cgtcgccccg cggcgcattg
      421 cggcggctct accgcaggta catttggggg gagcgattcg cgtcgtatca tcgcgacttg
      481 gtgaactcag agtttacccg aggctggatg aagcgcaggt ggttcatcga ctcgacggtg
      541 gtctatccgc cgtgtcggga tgccatcgca ccggcagaga agcgaccgat catcctggcc
      601 gtggggaggt ttgtgcccga caagaaggcg gatgtcctag tggaggcgtt tcgtacactc
      661 tgcgaccagg gactgcgggg ctgggagctc gtactggccg gcggccttcc cggtagcgag
      721 tgggaggggg ccgattccta tctcgagaac ctgaagcaac gggcgcaggg gtacccgatc
      781 gatttgcgga tggatgtccc cggccccgat ctgacgcgcc tgttcgagac ggcggccatc
      841 ttctggcatg ccaagggtta tcaagtcgat gcggaggtcc accccgagca catggagcat
      901 tttgggatcg tcaccgtgga ggcgatgatc gctggctgcg tgccgattgt cttcgcaggg
      961 ggcgggcagc cggagatcgt caacgatggg gccgacggcg tcctctggcg cacgcaggag
     1021 gaactggccg atcggaccag ggacctcatc agtaacccgg accgcctgcg ggagttgtcc
     1081 ggccgagccg taggacgggg caacacgttt agtaaagacg ctttcgtgtc taggtttatc
     1141 aacgcggtct cggatctgct tcctggccag cgggaccgcc tggcgctgca aagtaaatcc
     1201 tgatccgctc atgtggggaa acaggcgcaa gggcggcatc cgcaacagca ctctcataaa
     1261 ttcatccgtc actcctttcc cgcatccgcc tggcccggca tccttcgcct gttgattctc
     1321 ggcaacgccg cccgacttga gtgcacgtaa gctatcgaca gaatctggag tgcgctgtta
     1381 tcgttgacct cctccacgga gtagaattat cacggcgtcg cgaacgtact ccacagactc
     1441 gctggtaaaa tttatggcaa cgacggcatc gccgagcaac ttccagcaca tccagatccg
     1501 gtccgattcc aacctggcca ccctcgacct tcaagagatc ttccgctatc gggatctcct
     1561 gctgacgctg gccgatcgcg acatcagggt ccgttacaag cagacggtca tcggggtggc
     1621 gtgggtggtg ctccaacctc tgatgacgtc gctgatcttt gcgttcgtct tcggcgtgct
     1681 cgcccgcttg ccgagcgagg ggcggccgta catcctgttc gctttttcag gcttattggc
     1741 ctggaatgcc ttctcgcaaa cgctgacgag ggttagcttc gcgctggtgg gaaacgtcca
     1801 tctggtctcg aaggtgtatt tccccaggct gattctgccg ctcgccgcga tggcatcgac
     1861 gctggtcgat tttgttgtcg cgctggtgct gatgttcctc atgttgatct tctatggggt
     1921 atggccgggg tggggcctgc tgctgctgcc ggtctggctc gcggtaatgc tggtgatggc
     1981 gctgggcctt ggcctggtgg cggcggcact gatggtcaag tatcgcgaca tcagccacat
     2041 catcccgacg atccttcagt tgggcatgta tgtcaccccc gtggcatggt ccgcgctgat
     2101 tgtccccggg aaattccgct ggatcttcct ggcaaacccg ctgactggcc tgctgaatgc
     2161 attccgctgg tcgctgctgg gcgaaggggc gctttcgttg ccggcactgg cttattcgac
     2221 ggtgacggcc gccctgttct tctggtgcgg cgcgatcgtc ttcaagcagc aggagcgaaa
     2281 tttcgccgat gtcatctaac tccattgcca tcagcgtcaa gggactgggc aagaagtaca
     2341 cgatcgccca caagtcgacg cggccgacga cgctcaggga agccatcgtc gagcggttgc
     2401 ggcatccttt cggctatgac cgggaggagc atgagagttt ctgggcgctg cgggacgtcg
     2461 atttcgagat ccgcgagggt gaagtggtcg ggttcgtcgg ccgcaacggc gccggcaaga
     2521 gcacgctctt aaagctcctc tcgcggatca cggggccgac gaccgggaca atcgacatcc
     2581 acggccgaat cgcgagcttg ctggaggtcg ggaccggctt ccacccggaa ctgaccggcc
     2641 gggagaacat ttatctcaat ggggcggtcc tcggtatgag ccgtcgcgag atcacatcca
     2701 agtttgacga aatcgtggcc ttcgcggagg tcgagcaatt cctcgatacc ccggtgaagc
     2761 gctactcgtc ggggatgtac gtccggctgg ccttcgccgt cgccgcccac ctcgagcccg
     2821 agatcatgat cgtcgatgag gtcttgagcg tgggcgatgc ctcgttccag aagaaatgta
     2881 tgggcaagat gcaggacttc tcgggtcatg gccggacgat cctatttgtc tcgcacaaca
     2941 tgaatg
//
LOCUS       DATAQL010000142         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3985454, whole genome shotgun sequence.
ACCESSION   DATAQL010000142 DATAQL010000000
VERSION     DATAQL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3985454"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..776)
                     /locus_tag="VHS97_02360"
     CDS             complement(159..776)
                     /locus_tag="VHS97_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447064.1"
                     /translation="MLRKTAVGSLADHARSGLRRREILQIGYSSLIGAGLSTLLNARG
                     AAEARSTPMPRAKSVLLIFLTGGPCQLDTFDPKPDAPAEVRGPFGSIETKLAGVRFAG
                     LLPEMAARADRFALIRTLAYPDLPGASHEVATPLILAGLDQLPAGVRESDARRLWPCY
                     SGALNHFRPRQDGLPSGMVIPKPLRFVDGEALGSDAGLLGPRYDP"
     gene            1120..2535
                     /locus_tag="VHS97_02365"
     CDS             1120..2535
                     /locus_tag="VHS97_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3447065.1"
                     /translation="MRYALGRRSWLALLLAVTLSGSSKAQGTRDTTTYRRFKAALDAI
                     PAIDTHDHLLPFDRLLARRETDRGKGVNLAGLWQNSYYTWNHRLEPWTPGMKFDAWWQ
                     KAKHDFDDSRATSFYRYQLPAFTDLYGVDFETLTDEKARALDDQIFENYKDSTWLQKV
                     VTERANIELMFNDPYWDRFGFSTDYPFGVLVLNVTTLVAGFHPSEFKEPADDPYAFAV
                     RERMTVKSLDDYLKVLDRLFVKAREKGAVCLKTTLAYKRTLRFEDVPAERAAQAFGHP
                     RSELSAEQLKGFEDFIMWRLVELSARHELPFQIHTGQARIQGSNPMLLVDLIEANPKT
                     KFILFHGGFPWVGETGVILMKHGRHVWLDSVWMPTLSYETAKRAFHEWLDVMPSSRIM
                     WGADANHAEGIYGATEMTRRCLAEVLAERVDRGDLREEHALRIGRQILRDNALSLFPQ
                     LESRLWKHKQAVGTAQAPKAQ"
     gene            complement(2934..3131)
                     /locus_tag="VHS97_02370"
     CDS             complement(2934..3131)
                     /locus_tag="VHS97_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447066.1"
                     /translation="MLKFVMALGVIWWIVIAVWFVRLGLGLDHLSNDQILPGIIGGIC
                     GGWAAWYYVPRLMKKEAEKSR"
     gene            complement(3170..3352)
                     /locus_tag="VHS97_02375"
     CDS             complement(3170..3352)
                     /locus_tag="VHS97_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447067.1"
                     /translation="MKRLDLLVYVSVFAASYILFGLASGQTTFSRPDLAGMAAGCIGG
                     ELGMLYMRHRTKRQAS"
BASE COUNT          746 a         1106 c         1063 g          649 t
ORIGIN      
        1 cgaactggcc gatcgttgga tcatccgccg caacgcggcg ccagtcgtcg aatcgctcca
       61 acagggcgcg cctgtccctt gcgcgagagc ctgccatgcc gatcgagagt ccgacatttt
      121 ccgggccaaa agtcggatcg gtcggattac agttgagctc aaggatcata ccgtggccca
      181 agcaatccgg catcgcttcc gagagcctcc ccgtcgacga aacgtaaggg tttggggatc
      241 accatgccgc tggggaggcc atcctgcctc ggccggaagt ggttcagggc accggaatag
      301 cagggccaaa ggcgtcgcgc atcggactcg cgcacgcccg ccggcaattg atccaacccg
      361 gccaagatca atggcgtcgc gacctcatgg gatgccccgg ggaggtcggg ataggctaac
      421 gtccgtatca aggcgaaccg atcggctcgc gccgccatct cgggcaggag gccggcaaat
      481 cgcacgccgg caagcttggt ctcgatcgat ccgaaaggac cacggacctc ggcaggagcg
      541 tcgggcttgg ggtcgaaggt gtcgagctgg cacggaccac cggtcagaaa gatcagcaag
      601 accgacttcg ccctcggcat tggcgtcgat cgcgcctcag ccgccccgcg cgcgttgagt
      661 aaggtcgaaa gacccgcccc gatcaggctg gagtatccaa tttggagaat ctctcgtcgg
      721 cgcaagcctg atcgagcatg atcggccagt gatcctactg ctgttttgcg caacatgtcg
      781 tcctcgttcg cgcctagatc aactggtgaa gatccaacgt tactttgagc ctggagccta
      841 tcgcatcaag gcctgagtga aaacaccggc gtcgggggga tggaccactt caatgcccaa
      901 cctgcggtcg ttgcgccgac cgactccatt tttaccagaa atagaccaga accgctcaac
      961 tcgccatccg attccacatg cttgaacgct gcgccttcgg gaaatcgctc ggatctctcg
     1021 caagatcctc atagtttaca cggcgttccg gccggagtat cttccggtca gattgtccca
     1081 aagcgggacc tggctgcgac atcccacggg gtgatccaca tgcgatatgc actcggacgt
     1141 cggtcatggc tggccctgct gctcgccgtc acactctcgg ggtcgagcaa ggcgcagggg
     1201 acgcgtgata cgaccacgta tcgccgcttc aaggcggcgc tcgacgcgat cccggcaatc
     1261 gatacgcacg accatctcct gccattcgac cgtttgctcg ctcgccgcga aactgatcgc
     1321 ggcaagggtg ttaacctcgc cgggctctgg cagaacagct actacacctg gaaccaccgg
     1381 ctggagccgt ggacgcccgg gatgaagttc gacgcctggt ggcaaaaggc caagcacgac
     1441 ttcgacgact ctcgcgcgac gagcttctac cgctatcagc tccctgcctt cactgacttg
     1501 tacggcgtgg acttcgaaac cctgaccgac gaaaaggccc gcgcgctgga cgaccagatc
     1561 ttcgagaatt acaaagactc cacctggctg caaaaggtcg tcaccgagcg cgcgaatatc
     1621 gagttgatgt tcaacgaccc atactgggat cggttcggat tcagcaccga ctaccccttc
     1681 ggagtcctcg tgttgaacgt cacaacgctg gtggccggct ttcacccctc agaattcaag
     1741 gagcccgctg acgacccata cgccttcgcg gtcagggaga ggatgaccgt taaatcgctc
     1801 gacgactacc tgaaggtgct cgatcgcctg ttcgtgaaag ccagagagaa gggcgcggtc
     1861 tgtctcaaga cgacgctcgc ctataagcga acgctgcgct tcgaggacgt ccccgccgag
     1921 cgtgccgcgc aggcgtttgg gcatccccga tcggagcttt cggccgagca gttgaagggg
     1981 ttcgaagact tcatcatgtg gcggctcgtc gaactgagtg cccgccatga gctcccgttc
     2041 cagattcaca ccggccaggc gcggatccaa ggctccaacc cgatgcttct ggttgacttg
     2101 atcgaggcca atcccaagac gaagttcatc ctgtttcatg gtggattccc gtgggtgggc
     2161 gagacaggag tcattttgat gaagcacgga cgacatgtct ggctggactc ggtttggatg
     2221 ccgacactca gctacgagac cgcgaagcgg gcgttccacg agtggctaga cgtgatgccg
     2281 tcgagccgga tcatgtgggg ggccgacgcc aaccatgccg agggaatcta cggcgctact
     2341 gagatgactc gtcgctgcct ggctgaggtc ctcgcggagc gcgtcgaccg aggagacctt
     2401 cgcgaagagc acgcgttgcg aatcgggcgg cagatcctcc gcgacaatgc cctgtccctc
     2461 ttcccccagc tcgaaagccg actctggaag cacaagcaag cggtaggtac ggcccaggcc
     2521 ccgaaggcac agtgacgacg gcgagcacct cggaagtggg cgtgaaatct cgcgaacatg
     2581 agttggccag cattcggaca cgatacgagg acgcataatc gaggaccaga accttctaaa
     2641 gcgcgagggc aacattgaga tattctggga gggcgaggct ccgtccgagc tggcattgcg
     2701 gtgcggctcg gacgaagcgc ttgtagcgat ttcgttgacg gacgggagcc cgaaaagccc
     2761 cgaacgatcc atgttgtagc ctggggtgta aatcccaggt atcgaccgag ccaacattaa
     2821 acgataacag aattcaatgc cacggcccaa ccccggtcgc cgtccggggt ttacaccaca
     2881 ggctgcgctt tgtcgccgct ctgcggctct cacgagcgct cgagccacgc ttatcaccga
     2941 gacttctcag cttccttctt catcagccgc ggcacgtagt accacgcggc ccacccaccg
     3001 cagatgccgc cgatgatccc cggcaggatt tggtcgttgc tcaggtggtc gagccccaag
     3061 ccgaggcgga cgaaccacac ggcgatcacg atccaccaga ttacgcccaa agccatcacg
     3121 aacttcaaca tcggattctc ctcgcttggt cgtttgtcga taccatcggt caggacgcct
     3181 gacgcttcgt ccggtgccgc atgtaaagca tccccagctc gccgccgatg cagcctgccg
     3241 ccatgcccgc aaggtcgggt cggctgaacg tggtctgccc ggacgccagg ccgaacagga
     3301 tgtaactcgc ggcgaatacg ctgacgtaga ccagcaagtc gaggcgtttc ataactcact
     3361 ccttggggtt taaacgtcgt cggcgtctca ctaagggtta tcagcgtcgg ggtgaggaac
     3421 ggacagaaag ttcatgtagg atgcgcggat caaagcgatc gtcgatcacg ccgccgccgg
     3481 ccgttacaag gcgaagccat cgcgggtgta tcgcttcgag gacattcgcg aggcgcatcg
     3541 cgcgatggaa tcgaatcagg cgaa
//
LOCUS       DATAQL010000143         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5054392, whole genome shotgun sequence.
ACCESSION   DATAQL010000143 DATAQL010000000
VERSION     DATAQL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5054392"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..477
                     /locus_tag="VHS97_02380"
                     /pseudo
     CDS             280..477
                     /locus_tag="VHS97_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            478..972
                     /locus_tag="VHS97_02385"
     CDS             478..972
                     /locus_tag="VHS97_02385"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447068.1"
                     /translation="MFLATADRAMVKMNDRITASIASISDGLDRVAKAISTYLAFFAE
                     NPQFVELIIQERANFKDRKRPTYFEHRERNVERWRQLYRELMAEGRVRQMPAEQITDV
                     IFGAIYGTMFINYFTGQSKPSDVQAQEIVDIVFRGILSESERERRGGGGPVSPLATAK
                     TPNL"
     gene            1015..2316
                     /locus_tag="VHS97_02390"
     CDS             1015..2316
                     /locus_tag="VHS97_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447069.1"
                     /translation="MNRRASVLLLMLMAAISGCQGGSPAPPKSAPNVEFQMPVAGHVT
                     DYADFPGTTDAIVTVQVTSRVSGYMTKVYFKDGTPVEEGNLLFEIDPRQYKAEYDRAE
                     GNLAQIEAHMRRLNKEYDRARTLLIKRSISQEEYDRYESDFKETQANLEIAKANRDLA
                     KLNLDWTEVRAPSAGLLSRRMVDPGNLIKADETALTSIVTQDPMYVYFDVDEQNMLKV
                     HRLIREGKIKAKSEKEVPVLFGLSDETPNFPHGGTVDFTDNKVDINTGSLRFRATVRN
                     EKGILTPGLFVRVRLPIGDPHETLFVREEALASDQGRKIVYTVEWKPVFEDGKPVYVE
                     SGSGPEKTKEQKMGWIVAAKPLKVGSLRDGFRAVEDGLAPTDKVIVTGLQKIRPGREV
                     KPTEKPLDRDVPKDLSFNFPRGDENKLGGGSPGRDTRKVSP"
     gene            2434..>3455
                     /locus_tag="VHS97_02395"
     CDS             2434..>3455
                     /locus_tag="VHS97_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3447070.1"
                     /translation="MFSRFFIDRPIFAAVLSIIITLAGGLSLIALPIAQYPQVSPPNV
                     SVDCTYPGASAQVVAETVAAPIEQAVNGVENMMYMASQCTNDGSYNLTITFKLGVDLN
                     LAQVMVQNRVALALPIMPDVIKQTGVTTKKKAPDILMSVAIFSPDDRYDQLYLSNYAM
                     IQIQPELARLDGVSDVTMLGQRDYSMRIWVDPDKLAARNLTAGDVVAAIREQNLQVAT
                     GQVGQQPMTRGQRTQVATKTLGRLVDTEQFGNIIVKMMPDGHFLRLKDVGRVELGSKN
                     SDLDSEVDRKPSGSLAVFQLPDANALECADRVMAKMDELRKTFPDGLDYVVRYNTTPY
                     IRESIW"
BASE COUNT          764 a          999 c         1007 g          685 t
ORIGIN      
        1 cgaaaggatt ccgtctcgca attttcgagc cgccggaact cgtgagcttt agttgatgcg
       61 tctgggttgg cctacgcctt gccgccgtcc cccccctaat tccttatggt tgtgtggcga
      121 aatcgtgact gatgcgcccg gagactcaag cgttgcgctt gccggactct tgccgttgcg
      181 ctcaccccac cggaaaaatc ctcgcgatcg gccattgacc tgggcggggc ctcgcttaaa
      241 atgtggactg actggtcagt ccgcagtttt gaggggtgca tggaaaatct cagcgagaaa
      301 aaattgaagc gatgctgcga cgaggagtcc atattggcgc ggagggagga aatcctggac
      361 acggccaccg aactcttcgc cgaccaaggc ttctctgacg ccgtgacgca ggttttggcg
      421 gatcggcttc aaatcggcaa aggaactctc taccgccatt ttcccagcaa gcgcgatttg
      481 tttctggcga cagctgaccg cgccatggtg aaaatgaacg atcgaatcac tgccagcatc
      541 gccagcatca gcgatggtct ggatcgcgtg gcgaaagcca tctctaccta tcttgccttc
      601 tttgctgaaa atccgcagtt tgtcgaatta attatccagg agcgggcgaa cttcaaggat
      661 cgcaagcgcc ccacctactt cgagcatcga gagcgcaatg tcgagcgctg gcgccagttg
      721 tatcgggagc tgatggcgga gggacgggtt cggcagatgc ctgccgagca gatcaccgac
      781 gtgatcttcg gtgcgattta cggaacgatg ttcatcaatt atttcacggg tcagtccaag
      841 ccatcggacg ttcaggcgca agagatcgtg gatatcgttt ttcgcggtat tttgagcgaa
      901 tcggaacggg agcgtcgagg gggagggggg ccggtatcgc ccctggccac ggcaaagact
      961 ccgaacctct agacatacca accagtctga tcactctaag tggggaaaga atccatgaat
     1021 cgccgggcca gcgtgttgct actgatgctg atggccgcga taagcgggtg ccagggaggg
     1081 agcccggctc cgcccaagtc ggctccaaat gtcgaatttc aaatgccggt cgcgggccat
     1141 gtgaccgatt atgcagattt ccccggtacg accgatgcca ttgtcactgt tcaggtgacg
     1201 tcgcgcgtat ctgggtacat gacgaaggtg tatttcaagg atggcacgcc ggtcgaagag
     1261 gggaacctgc tcttcgagat cgatccgcgc caatacaaag ccgagtacga ccgagccgaa
     1321 ggcaacctgg cgcagatcga ggcccacatg cggcggctca acaaagaata cgatcgagcc
     1381 cgcacgttgc tgataaagcg ctcgatcagc caggaagagt acgaccgtta cgaatccgac
     1441 ttcaaggaaa cgcaagcgaa tctcgagatc gccaaggcca accgcgatct ggccaagctc
     1501 aacctcgatt ggaccgaggt gcgagcgccg tcggcgggtc tgctcagccg tcgaatggtc
     1561 gatcccggta acctgatcaa ggccgacgaa acggccttga cctcgatcgt cactcaggac
     1621 ccgatgtatg tgtatttcga tgtcgacgag caaaacatgc tgaaggtgca tcgcttgatt
     1681 cgtgagggga aaatcaaggc caaatccgaa aaggaagtgc ccgtgctctt cggcctgtcc
     1741 gacgagacgc ccaatttccc ccacggcggc acagtcgatt tcaccgacaa caaggtcgac
     1801 atcaataccg gcagcctgcg gttccgagcc acggtaagga acgaaaaggg aattcttacg
     1861 cccgggctgt tcgtgagagt ccggttaccg atcggcgacc cccacgaaac gctgttcgtc
     1921 cgcgaggaag ccctggcgtc agatcaaggg cggaagattg tctataccgt cgagtggaaa
     1981 cccgtgttcg aggacggcaa gccggtttat gtcgaatcag gatcggggcc cgaaaaaacc
     2041 aaagaacaaa agatgggctg gatcgtcgct gccaagcccc tcaaggtcgg ctcgctccgc
     2101 gacggcttcc gagccgtcga ggatggcctg gctcccaccg ataaggtgat cgtcaccggc
     2161 ttgcagaaaa tcaggccggg cagggaagtt aagccgaccg aaaagcctct cgatcgcgat
     2221 gtccccaagg atctgtcatt caacttccca cggggagatg agaacaagct gggtggcggc
     2281 tcccctggac gtgatacgcg caaagtctct ccatgagttc cactccctcc ggcatccgcc
     2341 aatccagggt tcgctcgaca cgcccgcggg ccctggttgg tttactgcgt ccttggtcgt
     2401 tggtatcagg aaacctgatc gagagactca accgtgtttt ctcgtttctt catcgatcgg
     2461 ccgatcttcg ccgcggtgct gtcgattatc atcaccctgg ccggtgggct atcgctgatc
     2521 gcgttaccca tcgctcaata cccgcaagtt tcgcccccga acgtctccgt tgactgtacc
     2581 taccctgggg ccagcgccca ggtcgtcgcc gagacggtgg ccgcgccgat cgagcaagcg
     2641 gtcaacggcg tcgaaaacat gatgtacatg gcgtcgcaat gcaccaacga cggctcgtac
     2701 aacctcacga ttaccttcaa gctcggagtc gatttgaatc tggcgcaggt gatggtccag
     2761 aaccgagtcg ccctcgcctt gccgatcatg cccgatgtga tcaagcagac cggcgtgacc
     2821 accaaaaaga aggcgccgga cattctgatg tcggtcgcca tcttttcgcc tgatgatcgc
     2881 tacgaccagc tctatctcag caattacgcc atgattcaga tccagcccga gctggcccgg
     2941 ctggacggcg ttagcgacgt gacgatgctc ggccagcgcg actacagcat gcgaatctgg
     3001 gtcgatccgg ataagttggc cgcccggaat ctgacagccg gagacgttgt cgccgcgatc
     3061 cgcgagcaaa acttgcaggt ggccactggc caggtcggcc agcagccgat gacacgcggc
     3121 cagcggactc aggtcgcgac caagaccttg ggacgactcg tcgatacgga acaattcgga
     3181 aacatcatcg tcaagatgat gcctgacggc cattttctgc ggctcaagga tgtcggccgg
     3241 gtggaacttg gttccaagaa ttcggatctg gacagtgagg tcgatcgtaa acccagcggc
     3301 agtctggcgg tgttccagct ccctgacgct aatgcgcttg agtgcgccga tcgggtcatg
     3361 gccaagatgg atgagctcag gaagaccttt ccggacggtc tcgattacgt cgtccgctac
     3421 aacaccacgc cctacatccg cgaatcgatc tggga
//
LOCUS       DATAQL010000144         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1848166, whole genome shotgun sequence.
ACCESSION   DATAQL010000144 DATAQL010000000
VERSION     DATAQL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848166"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1754)
                     /gene="purH"
                     /locus_tag="VHS97_02400"
     CDS             complement(153..1754)
                     /gene="purH"
                     /locus_tag="VHS97_02400"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX3447071.1"
                     /translation="MNEAIIPIRRALISVFNKQGLIDLARALAATGAELLASGGTRSA
                     LAASGLDVIEVADYTGQPEVFGGRVKTLHPKIHGGILARRDDPGDLATLESQGFLPID
                     LVVVNLYPFEATISRPDVSQAEAIENIDIGGPALIRAAAKNHAHVAVATNPSQYPALI
                     SAIREKGGTNLTDRHGLAVEAFRTTSLYDSAIAAYLGRVMSTQAPEPIAFPDRLQLRF
                     ELRQPLRYGENPHQQAAFYVEPAERGTNLATAKLRHGKELSYNNLLDLDSALRLVRQF
                     SDPAACILKHNNPCGAAIADDLATAFENAYDGDSLSAFGGVVGLNRPVDLLSAQRMSK
                     PGRFLEAILAPGFDEDALEWLRTKPSWRNSVRILDLGGSLGPTGDAPTRLDYRRIEGG
                     LLVQSWDQLEPDPAAGTIATRRSPTDLERRDLAFAWKVCASVKSNAIVLAKDRRIVGV
                     GAGQMSRIDSVRIATEKAGPRAAGSVLASDAFFPFRDGPDVAAAAGVTAIIQPGGSRR
                     DDETITACDEHGMAMILTGRRHFRH"
     gene            complement(1883..3253)
                     /locus_tag="VHS97_02405"
     CDS             complement(1883..3253)
                     /locus_tag="VHS97_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3447072.1"
                     /translation="MFRRLLSFWVFVIGTLAGGSARAQMPFPVDLIPTRTALERLGLE
                     RQWFGVIPLAESERLKRITMSGDRLFAQTDHAMVHAFDSESGRLLWSAQLGERTGFAR
                     GVASNSFAAFVTNANTLHALDKNTGRPIWKQNLNTIPTSTPACDENQVSVGMTSGRVV
                     GFNLKLRDDKGNESILREPSQAWAWTTGGPVLTRPLPAGQFVAFGSSDGKAYVVAANE
                     WTSLYRIATGGPIGEGLGAYGTRTLLIPSGDNNLYGVDLLTSRVLWTFPSGAPIDQEP
                     LVADSDIYTVNTAGNLSVIDPNTGAPRWTTSTQGGRLAAISGAKLFLRSYNLDLFLMD
                     RKTGRMLVDPGETHVRAGLNLREYDLDVVNRFNDRMYFATSSGMIVCLRETGHAQPRL
                     LRDPSALPFGYVPPEGVKETPPPAPAAETGAQPKNEPGAEATPPAGEKEKADTDKEKV
                     PAEAPK"
     gene            complement(3431..>4014)
                     /locus_tag="VHS97_02410"
     CDS             complement(3431..>4014)
                     /locus_tag="VHS97_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447073.1"
                     /translation="PVLAGERAHSIADIARAIRAANWVDAQTRLDELEESFPNDPELS
                     ILREQLARARHDAIQVTLSQLAAAREVNDPDRVLEVYQVVFPLLDQPERIALERDLAK
                     WFLTLIHRRLRVGKVQPDVVHLASRFSESFASTVEGASVRASLSTLRRSVGLCPRCGH
                     PYTGIADACPKCLKPSSNSSATILGGAEMNEPA"
BASE COUNT          775 a         1226 c         1228 g          785 t
ORIGIN      
        1 gggggctggg gtcgagctgg cgacacccag cgacccccgg cgcggaagcc tcggatctgg
       61 gggcgtcgcc cagcgggcgt cgacccagcc acccttgagg tgcaacctgg ttgctggctg
      121 tgccgaccac acgtaccgca agcgggcatc tctcagtgcc ggaagtggcg tcgtccagtc
      181 aggatcattg ccatcccatg ttcatcgcag gcggtgatgg tttcgtcgtc cctcctggag
      241 ccgcctggtt gaataattgc ggtcacgccg gcggcagcgg ctacgtccgg cccgtcgcgg
      301 aacgggaaga atgcgtcgga agccaaaact gagccggcgg ccctggggcc ggctttttcc
      361 gtcgcgattc gcaccgaatc tattcgactc atctgcccgg caccgactcc gacgatccgt
      421 ctgtctttcg ccagcacgat cgcgttggac ttgaccgacg cgcagacttt ccaggcaaac
      481 gccaggtcgc gccgctctaa gtccgtcggc gatcggcgcg ttgcaatcgt acccgcggcc
      541 ggatccggct ctagctgatc ccagctctgc acgagcaggc ctccttcgat gcgccggtag
      601 tccaatcgag tgggggcgtc gccggttggg ccaagcgaac cgccgagatc gagtatacga
      661 acgctgttgc gccagctcgg cttggtccgg agccactcca gtgcgtcttc gtcaaagcca
      721 ggggccaata tcgcttcgag aaatcgaccg ggcttggaca ttcgctgcgc gctcaatagg
      781 tcgacaggac ggttcaatcc cacgactccc ccgaaagcac tgagcgaatc gccgtcgtaa
      841 gcattttcga atgccgtggc caggtcatcg gcgattgcgg cgccgcacgg gttgttgtgc
      901 ttgagaatgc acgccgccgg atcggaaaac tggcggacca gtcgtaaagc actgtcgaga
      961 tcgagcaagt tgttgtagga gagttccttt ccgtgacgca gtttcgctgt cgccaggttc
     1021 gttccacgct ccgctggctc gacgtagaac gccgcttgct gatgcggatt ctcgccgtaa
     1081 cgcaaaggct gtcgtaactc aaaacgcagt tgaagccgat cgggaaaagc gatcggttcc
     1141 ggggcctgtg tgctcatgac gcggccgagg taagcagcga tggccgagtc gtagagactg
     1201 gtcgtgcgaa aagcctcgac cgccagcccg tgccggtccg tgagatttgt ccctcccttt
     1261 tcccggatgg ccgagatcaa ggccgggtac tgagaagggt tggtcgcgac cgcgacgtgt
     1321 gcatggtttt tggccgccgc gcgaataagc gccggcccgc cgatgtcgat gttttcaatc
     1381 gcctcggcct ggcttacatc aggccgggag atggtggcct caaaaggata caagttgacg
     1441 acgaccaggt cgataggcag aaacccctgg ctctcgagcg tggcaaggtc tccgggatcg
     1501 tcacggcggg cgagaatccc accgtgaatc ttggggtgta aggtcttgac cctacccccg
     1561 aaaacctctg gttgaccggt ataatcggca acctcaatta cgtccaaacc ggatgccgcg
     1621 agcgctgacc gcgtgccgcc gcttgccagc aactctgcgc cagtcgccgc taatgcccgc
     1681 gccagatcga tcaagccctg cttattgaag acactgatca atgctcgccg gatggggatg
     1741 atcgcctcgt tcatggttgc aactcccgga tttcgactca aagccgtcgt accaacgaaa
     1801 accccctcat cgggccttcg gccaaccatt ctcccacatt gggagcggtt ttcgatgagg
     1861 gggcacgtca atccgggata tgttacttgg gcgcctcagc gggaaccttt tctttgtcag
     1921 tatcggcctt ttccttctcg ccagcaggcg gagttgcttc ggcccccggt tcgttcttgg
     1981 gctgggcacc ggtctcggcc gccggagcag ggggaggcgt ctctttgacc ccttccggtg
     2041 ggacgtagcc aaagggcaga gcactgggat cgcgaagcag tcggggttga gcatggcccg
     2101 tctcccgcag gcacacgatc atccccgacg aggtcgcgaa gtacatccgg tcgttgaacc
     2161 gattgacgac gtccagatcg tattctcgca gattcaatcc ggcccggacg tgagtttcgc
     2221 cggggtccac cagcatgcgg cccgtcttcc ggtccatcag aaaaaggtca aggttgtacg
     2281 accgcaggaa gagtttggcg ccgctgatgg cggcaaggcg tccgccttgg gtcgaagttg
     2341 tccagcgggg cgcgccggta ttggggtcga tcacgctcag attgcctgca gtattgacgg
     2401 tgtaaatgtc cgagtcggcg accagcggtt cttggtcgat cggcgcaccc gagggaaacg
     2461 tccagagcac gcgcgacgtg agcaaatcga ctccgtaaag attgttatcg ccggagggta
     2521 tcagcagtgt tcgagtcccg tatgctccca gtccctctcc gatgggcccg ccggtcgcga
     2581 tacggtaaag cgaagtccac tcattggccg cgacgacgta agccttgccg tccgacgagc
     2641 cgaaggcgac aaactggccc gccggcagtg gtcgcgtcag gaccgggccg cccgtggtcc
     2701 aagcccaggc ctggctcggt tcgcgcaaaa tcgattcgtt ccctttgtcg tctcggagct
     2761 tgaggttgaa accgacaacc ctgccggaag tcattccaac cgaaacctga ttttcatcgc
     2821 aggcgggtgt gctcgtcggg atggtattca ggttctgttt ccagattggt ctgccggtgt
     2881 tcttgtccag cgcgtgcaga gtgtttgcat tggtgacaaa ggctgcaaat gagttggacg
     2941 cgacgcctcg cgcgaaccca gtgcgctcac cgagctgagc tgaccaaagc agccgccctg
     3001 actcggaatc gaacgcatgc accatcgcgt gatccgtctg ggcaaacagc cgatcccccg
     3061 acatggtgat gcgcttgagt cgttcgctct ccgccagagg gataactcca aaccattggc
     3121 gttcgagtcc gagccgctcg agcgcggtcc tggtcggaat gaggtcgacc gggaacggca
     3181 tttgtgccct cgcggacccg ccggccaagg tgcctatcac gaagacccaa aacgagagca
     3241 gtcggcggaa catgggctct ccctttcgca agctgatgag caagacaagc gcgcgcaaaa
     3301 ataaccacca ttcatcccta gccgacgact tcgcccacgt caagttgagt ggagctggag
     3361 attctaggta atttcgttcc atcgcaacca atcggcgtat tgagaccttc tggcgtccgg
     3421 ccaagaatgg ttatgccggc tcgttcatct ccgcccctcc gaggattgtc gcagacgaat
     3481 tcgacgaagg cttgaggcac ttgggacacg cgtccgctat tccagtgtac ggatgaccac
     3541 agcgtggaca aagcccgaca ctccgcctca gagtcgaaag tgaggctcgc acgctggcgc
     3601 cttccaccgt ggacgcaaag gattccgaga accgcgaggc aagatgcacc acgtccggct
     3661 ggaccttgcc tacacgtaag cggcggtgga tcagcgtgag gaaccatttg gcaaggtctc
     3721 gctcgagcgc gatgcgctct ggctgatcca gcaacggaaa gacgacctgg tagacttcga
     3781 gcacgcggtc gggatcattt acctcgcggg ccgccgcaag ctgagacaat gtcacctgaa
     3841 ttgcgtcatg ccgtgcccgg gccaactgct cacgcaagat ggaaagctcg ggatcgttgg
     3901 ggaatgactc ctccaattca tccagtcgag tctgggcatc cacccagttc gcagcccgaa
     3961 tggcacgcgc gatatccgcg atcgagtgtg cgcgctcacc tgccagcact ggat
//
LOCUS       DATAQL010000145         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3374088, whole genome shotgun sequence.
ACCESSION   DATAQL010000145 DATAQL010000000
VERSION     DATAQL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3374088"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1212
                     /locus_tag="VHS97_02415"
     CDS             73..1212
                     /locus_tag="VHS97_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3447074.1"
                     /translation="MSINWDSLRATEFPVAEHWAYFDHAAVAPLPQRSAEALRGFAHE
                     QLVDGVVGWPARERRIEAIRDQVALLINAHRDEIAFIPSTTHGIGLIAEGFPWREGDN
                     VVSAAEEYPSNLYAWMNLESRGVKLRLVPSRDGRIWLDDLASAIDGSTRVLTISHVEF
                     STGFRNDLDALAELCHKRRVALFVDAIQGLGPLVIDVQKTPVDFMAADGHKWLLGPEG
                     AGLLFVRREWIDRLRPIGIGWHSVVGSYNSPRNDFRLKDSAQRWEGGSFNMAGLLTFG
                     ASVGLFLELGPEAVSRRILERAEAVRTLAADAGWTVYGSARETDRSAIVALERPGVDP
                     DKAARALRGQGIVVCSRRGRLRVSPHLYNNHEDLERLKAGLAGSC"
     gene            1250..1786
                     /gene="coaD"
                     /locus_tag="VHS97_02420"
     CDS             1250..1786
                     /gene="coaD"
                     /locus_tag="VHS97_02420"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX3447075.1"
                     /translation="MKPISTDPLRTAVFTGTFDPITLGHLDVIGRGCLLFEHLVVAIG
                     VNPNKTSLFTIEERVEMARHVVRPFPNVTVKSFEGLTVHFVKRIGARVILRGLRTLSD
                     MEYEFGMTLTNHRLDPSVETVFLMADGEYSHVSSTLIKQVACYGGTEALKRFVPEELI
                     VPILSKLQINSPPAAGNE"
     gene            complement(1760..2461)
                     /locus_tag="VHS97_02425"
     CDS             complement(1760..2461)
                     /locus_tag="VHS97_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX3447076.1"
                     /translation="MALFSFEIDPHKVLGVTAQASLQEIRDAYKQKAKRFHPDGGGEE
                     WAFRILVQAYEMLSSARIARATRVETPPPAAHAPRSKPERGTESVHPGIHDSGVEPFR
                     VVGVELLCVRYLWDDAEYLWLTQRAPDEDRFLSCNLNLSWPDQGVVERDEMLRDQAAI
                     LTTLHEIFDQMIISTRVVASRSRAEADRFAGWLAYSNFDRTWKAVNTLHQLLRARGLG
                     LRQWSRDLFIPRGWR"
     gene            complement(2513..>3587)
                     /locus_tag="VHS97_02430"
     CDS             complement(2513..>3587)
                     /locus_tag="VHS97_02430"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEX3447077.1"
                     /translation="SPRMTLEDLTKSSGEWLRGTGPESDIVMCSRIRLARNLADFPFS
                     NRASRGEKAEIEAHFSSAMAHAKLDLSYLDVNSLPTLDRQFLVERQIISRELANSDGP
                     RGVAISLLENVAIMVNEEDHLRVQFMLSGFRLSEVWDAVNQLDDKLEDQLAYAFSPQL
                     GYLTACPTNVGTGIRVGVMLHLPGLVQTKQIDKVFRALQKINLAVRGLYGEGSQASGD
                     FYQISNQQTLGKSELELIKILTDVVPQVLTYERSARQALLTERRQHLHDQVSRAYGVL
                     KTAQTISSEETMLLLSSVRMGINLGLIDDLSIATVNELFIQTQPAFLQKLRGSELGVE
                     ERNVARASYLRSRLSNGRQAPRH"
BASE COUNT          646 a         1105 c         1136 g          700 t
ORIGIN      
        1 tcatcgtggg taagtctgcc aggcgctgac ggtccgacct ggtcgctggg ctaggcgggc
       61 gagcgcatct tcatgagcat taactgggat tccctgcgag ccaccgagtt cccggtcgcg
      121 gagcactggg cctatttcga ccacgccgcg gtggcccctc tgccccagcg ttcggccgag
      181 gccctccgtg gcttcgccca cgagcagcta gtcgatggcg tcgtgggctg gcccgcccgc
      241 gaacggcgga tcgaggccat ccgcgaccag gtcgcccttc tcatcaatgc gcaccgcgac
      301 gaaatcgcct ttatccccag cacgacccac ggcattggcc tgatcgccga ggggtttccc
      361 tggcgcgagg gagacaacgt cgtctcggcc gccgaagaat atccctcgaa cctttacgcc
      421 tggatgaacc tggaaagccg gggcgtcaag ctgcggctgg ttcccagccg cgacggccga
      481 atctggctcg atgacctggc ttcggccatc gatggttcga cgcgcgtgct cacgatcagt
      541 cacgtcgagt tttccaccgg ctttcgcaac gatctggatg cgctcgccga gctctgccac
      601 aaacgtcgcg tggcgctttt cgttgacgcg atccagggtc tggggccttt agtaatcgat
      661 gtacaaaaaa cgccggtcga tttcatggcg gcggacggtc acaagtggct cttaggaccc
      721 gagggggctg gcctactctt cgtgcgccgc gagtggatcg accggctcag gccgattggg
      781 atcggctggc atagcgtcgt cggctcgtac aactcgccgc gcaacgactt tcgcctgaaa
      841 gacagtgccc agcgctggga agggggctcg ttcaacatgg cgggcctgct cacttttggt
      901 gcgagcgtgg gactgtttct cgagctcggg cccgaagccg tgtcgcggcg gattctcgag
      961 cgtgccgagg ccgttcgcac tttagccgcc gacgccggct ggacagttta tgggtcggcg
     1021 cgcgagacag accgctctgc catcgtggcg ctcgagcgcc ctggggtcga tcccgacaag
     1081 gcggcccgcg cactgcgcgg ccagggaatc gtcgtctgca gccggcgggg acgccttcgt
     1141 gttagcccgc acctttacaa caatcacgaa gacctggagc ggctcaaagc cggcctggct
     1201 ggatcgtgct gatttccagt cgccccgcgt acgaagcccc gagatcgcca tgaaacccat
     1261 ttcgaccgac ccgcttcgca cggctgtgtt cacgggcact ttcgacccga tcaccctggg
     1321 tcatctcgac gtgattggcc gcggctgcct gttgttcgag cacctggtgg tcgccatcgg
     1381 cgtcaatccc aacaaaacct cgttattcac tatcgaagag cgcgtcgaga tggcgcgcca
     1441 cgtcgtccgc ccgtttccga acgtgacggt caagtcgttc gaggggctga ccgtgcactt
     1501 tgtgaaacgc atcggcgcca gggtgattct ccgcggacta cgtaccctct ccgacatgga
     1561 gtacgagttt ggtatgacgc tgaccaacca ccggctcgac ccctcggtcg agacggtctt
     1621 cttgatggcc gacggcgagt actcgcacgt ctcgagcacc ctgatcaagc aggtcgcctg
     1681 ctacggcggg accgaagcct tgaaacgctt tgttcccgaa gagctgatcg tgccgatcct
     1741 gtccaagctc cagatcaatt caccgccagc cgcggggaat gaataggtcg cgcgaccact
     1801 gccgcagacc caggccgcgg gctcgcagca gttgatgcag cgtgttaacg gctttccacg
     1861 tacggtcgaa attggaatag gccagccagc cggcaaagcg atctgcctcg gcgcgcgacc
     1921 gcgacgcgac cacgcgtgtg gaaatgatca tctggtcgaa gatctcatgc agcgtcgtca
     1981 gaatcgccgc ctgatcacgt agcatctcgt cgcgctcaac gactccctgg tcgggccagc
     2041 tgagattcag gttgcagctt aagaaacggt cttcatctgg agcgcgctgc gtgagccaaa
     2101 ggtactcggc gtcgtcccag agataacgca cgcacaagag ctcgacaccg acgacgcgaa
     2161 aaggctcgac gccgctgtcg tgaattccgg gatggaccga ctcggtgcct cgttccggct
     2221 tggaacgagg tgcgtgggcc gcgggcggcg gcgtttcgac gcgtgtcgcg cgggcgatgc
     2281 gcgctgagct gagcatttcg taggcctgaa ctaggatgcg aaaggcccat tcctccccgc
     2341 cgccatcggg atgaaatcgc ttggccttct gcttgtaggc gtcacggatc tcctggaggc
     2401 tggcctgtgc ggtcaccccg agcaccttgt gcggatcgat ctcgaaactg aacagagcca
     2461 caatatggtg tccttcgggg ggcgcctctc cctgctggtc ccgtgatcga gctcaatggc
     2521 ggggagcctg ccggccattg gacaagcggc ttcgcagata cgatgctcgg gcgacgttgc
     2581 gttcttcgac gccgagctct gatcctcgca gcttttgcag gaatgccggt tgcgtctgaa
     2641 tgaagagctc attgacggtg gcgatcgaca ggtcatcgat caacccgagg ttgattccca
     2701 tgcgcacact tgagagcaag agcatggtct cttcgctgga aatcgtctgg gcggtcttca
     2761 agacgccata agcgcggctg acctggtcgt gcaagtgctg ccgccgctcg gtcaggagag
     2821 cctgccgggc ggagcgttcg tacgtcagga cctggggaac cacgtctgtc agaatcttga
     2881 tcagctcaag ctcgctcttg cccagggtct gctggttaga aatctggtaa aagtcgccgg
     2941 atgcctggct cccctcgcca taaaggcccc ggaccgccag gttgattttc tgcaaggcgc
     3001 gaaacacctt gtcgatctgc ttggtctgga ccaagccggg gagatggagc atcacaccga
     3061 cgcggatccc ggtgccgacg ttggtcgggc acgcggtgag atagccgagc tgcgggctga
     3121 atgcataggc cagttggtcc tcgagcttgt cgtcaagctg gttgaccgcg tcccacactt
     3181 ccgacagccg gaagcccgag agcatgaact ggacacggag atggtcttcc tcattgacca
     3241 tgatggcgac gttttccagc aagctgatag cgacgcctcg cggaccgtcg ctgttggcaa
     3301 gctcgcgcga gatgatctgg cgctcgacca aaaactggcg atcaagggtg gggaggctgt
     3361 tgacgtcgag ataagagaga tcgagcttgg cgtgcgccat ggcggagctg aagtgggcct
     3421 cgatctcggc tttctcaccg cggctggctc gattggaaaa agggaaatcg gccaggtttc
     3481 gggccagccg gatccgagag cacatcacga tatcgctctc ggggccggtg ccccgcagcc
     3541 actcgccgga cgatttcgtc aggtcttcga gagtcatgcg tggggat
//
LOCUS       DATAQL010000146         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3374095, whole genome shotgun sequence.
ACCESSION   DATAQL010000146 DATAQL010000000
VERSION     DATAQL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3374095"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1485
                     /locus_tag="VHS97_02435"
     CDS             304..1485
                     /locus_tag="VHS97_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447078.1"
                     /translation="MEQRDDPKLNEKALEKAEFVEYRFPNKTGQSRFVHTPDFDGHGN
                     VFIMDVNQPAVVKVDPQTGKIDDHQNLGTSETLTVDNDGTVWFGSLNHFDPKTNALEQ
                     YRFDAGGGQGKSIGISTVIMDSQGNIWLSLLGTGGVGEFIRKEDTIRWWRVPVLRSHA
                     YGITLDHNDKVWMADYHDSGITSFDPTTGAFRHYLLDGDNTPSNIRRPGVDSKGFVWA
                     STWGSRGAMNATLYRVDPASGEVTPHKIGIPYANPYDVEADPWDNMWLSMDNYVVKFD
                     QKTNEFTRYPLPERTDVPKLAVTRDGAVWFGPRNAGEFGDYGGSASVLYPDKDKIKTF
                     AAYYFPGSDRDHKADYHGPGTPVTNTVKLVPAAEQNPGAYATALLGSESAAQAAGVAK
                     H"
     gene            1546..1911
                     /locus_tag="VHS97_02440"
     CDS             1546..1911
                     /locus_tag="VHS97_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEX3447079.1"
                     /translation="MKGSTSEERNSGSGCNRRLFLIRSSITLGAGALALTLGQRALAG
                     DPLLSVSDPEAQKLHYTDDASKVDHAANPTYVAGSKCANCSMFRGGGAATGGCALFQG
                     KSVSANGWCSGYSQQQDAE"
     gene            1912..>3154
                     /locus_tag="VHS97_02445"
     CDS             1912..>3154
                     /locus_tag="VHS97_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX3447080.1"
                     /translation="MDKFDLLIRNVRVVRPGASDVPLQQIAVKDGRFAAVAADLKPQS
                     ARTVTDAKGALGFPGLVDAHMHTGIYNPMEEDALRESRAAAQGGVTSSLNYFRSGQYY
                     MNRSGPYAELFPDLMEASAGRFYVDYAYHLAPMDAQEIGEISMLIEKYGVTSFKIFMF
                     YGGYGLHGASGHQHEFLMIGPDEKYDLAHFEFVMRGIKEAMERFPERRPYISLSMHCE
                     TAEIMAAYTKLVQKEGRLTGLRAYSAARPPHSEGLAITIASYLAQETDCLNINLLHLS
                     SRKAVEAALAMQGAFPQINFRREVTIAHLCIDYDVETGALAKVNPPIRSREDVEYLWK
                     TLLDGKIDWVCSDHACCRHEMKVDGTKPDDIFLAKSGFGGTEYLLSALITEGRKRGLS
                     YNRVAELTSRNPAQRFGLLRKG"
BASE COUNT          640 a          929 c          987 g          598 t
ORIGIN      
        1 atcgtgccct tcgacgtcgc ctatcccccg ggtcacggtc gggatgtgat tcagcgggta
       61 tgttttggct gccacaccgt caatttctac ccgtacaacg tcgttcgaag atacgccggt
      121 ggacgccctc cctttgacaa ggccggatgg gcgggcgtca tcgacctcat gtccaggaat
      181 ccgtgtttca atgtcccggg caatgcctcg tgcttcgatc ccgctctgct gtcgcccgcc
      241 gaccgcgagg cggtgattga ctaccttgct gccaatttcg gtgcgaattc cgtgccgcga
      301 gtcgtggagc agcgggacga cccgaagctg aatgagaagg cgctggagaa agcggaattc
      361 gtcgagtatc gcttccccaa caagacgggg cagagtcgct tcgtgcacac gcccgatttc
      421 gatgggcacg gcaatgtctt catcatggat gtcaaccagc ccgctgtggt caaggtcgac
      481 cctcaaacgg gcaaaatcga tgaccatcag aacctgggca cctcggagac cttgacggtc
      541 gataacgatg gtacggtctg gttcggcagc ctgaaccatt tcgatccgaa gacgaatgcg
      601 ctggagcaat accgcttcga cgctggcggt ggtcagggca agtccattgg gatcagcacg
      661 gtgatcatgg attcgcaggg caatatctgg ctgtcgctgc tgggaaccgg cggcgtcggt
      721 gagttcatcc ggaaggagga tacgatccgg tggtggcggg ttcccgtcct gcgcagccat
      781 gcctacggca ttacgctcga tcacaacgac aaggtctgga tggccgatta tcacgacagc
      841 gggatcacga gtttcgaccc gacaaccgga gccttccggc attacctcct ggacggtgac
      901 aacacgccgt cgaacattcg tcgccccggc gtcgactcga agggcttcgt ctgggcttcg
      961 acctggggta gtcggggcgc catgaacgcg acgctatatc gggtcgatcc ggccagcggc
     1021 gaagtgaccc ctcataagat tggaattccc tacgcaaacc cctacgacgt cgaagcggat
     1081 ccctgggaca acatgtggct ctcaatggac aattacgtag tgaaattcga tcagaagacg
     1141 aatgagttca cccgctaccc gctgcccgag cgaaccgatg tgccaaagct ggcggtcaca
     1201 cgcgatggcg ccgtctggtt cggtccgcgc aacgcggggg aattcggcga ctacggcggc
     1261 tccgcatcgg tgctgtatcc ggacaaggac aagatcaaga cttttgcggc ttactatttt
     1321 ccgggtagcg atcgcgacca caaagcggat tatcacggcc ccgggacgcc ggtgacgaac
     1381 acggtaaagc tggtccccgc cgcggagcag aacccgggcg cgtatgcgac tgctttactg
     1441 ggtagcgagt ccgccgccca ggcggcgggt gtggccaagc attgagcgct aaccgaagta
     1501 tgatggatag accatctcaa cggtccacga ttgaggattg agcaaatgaa agggtctacg
     1561 agcgaagagc ggaactccgg atccggatgc aaccggcgtc tttttctgat ccgtagcagc
     1621 atcacgctcg gagcaggcgc gctcgccttg accttgggtc agcgcgcgct tgcaggcgat
     1681 ccactgctgt cggtgagcga tccagaagcc cagaagctgc attacaccga cgatgccagt
     1741 aaggtggatc acgccgcgaa cccgacctac gtggccggtt caaaatgcgc caactgcagc
     1801 atgttccgcg gcggcggcgc ggcaacagga ggctgcgcgc tcttccaggg aaagtcggtc
     1861 agtgcaaacg gttggtgttc gggttactcg caacagcagg atgcggaata ggtggataag
     1921 tttgatttat tgatccggaa cgtccgtgta gtgcgtccgg gcgccagcga cgtgcctttg
     1981 cagcagatcg ctgtgaagga tggccgattt gccgcggtgg cagcggacct caagccgcag
     2041 tcagcgcgta ccgtcaccga cgccaaggga gcgctcggct tcccggggct cgtcgatgcc
     2101 catatgcaca ccggtattta caacccgatg gaggaggacg cgctgaggga gagccgcgcc
     2161 gcggcgcagg gcggggtgac ctcgagcttg aattatttcc gctcggggca gtactacatg
     2221 aacaggagcg ggccttacgc cgagctgttt cccgatctca tggaggcgtc ggcaggtcgc
     2281 ttctacgtcg actacgcgta ccacttggct cccatggacg cgcaggagat cggcgagatc
     2341 agcatgctga tcgagaaata cggcgtcacc tcgttcaaga tcttcatgtt ctatggaggc
     2401 tatgggctgc acggtgcctc ggggcaccag catgagttcc tcatgatcgg gccggacgag
     2461 aagtacgacc tcgcgcactt cgaattcgtg atgcgcggca tcaaggaggc gatggagcgg
     2521 tttcccgaga ggcgccccta tatctcgttg agcatgcatt gcgagaccgc cgagatcatg
     2581 gcggcttaca cgaaactcgt tcagaaggag ggccgtctca cggggttgcg cgcctacagc
     2641 gccgcccggc cgccgcactc ggaaggcctg gcgatcacga tcgcctcgta tctcgcgcag
     2701 gagacggact gtctcaacat caatctgctc catctttcct cgcgcaaggc cgtcgaggcg
     2761 gcacttgcga tgcagggcgc atttccgcag atcaatttcc ggcgggaggt gaccatcgcc
     2821 cacctttgca tcgactacga cgtcgagacc ggagctctgg cgaaggtgaa tccaccgatc
     2881 cggtcacgcg aggatgtcga gtacctctgg aagactctgc tcgacggcaa gatcgactgg
     2941 gtctgcagcg atcatgcgtg ctgccgtcat gagatgaagg tcgacggcac caagccggac
     3001 gacatcttcc ttgccaagtc gggattcggc ggcacggaat atctgctgtc ggcgctcatc
     3061 acggaaggac gcaagcgcgg cctgtcctac aaccgcgttg cagagctgac ctcccgaaac
     3121 ccggcccagc gcttcggact gctccggaag ggcg
//
LOCUS       DATAQL010000147         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5359714, whole genome shotgun sequence.
ACCESSION   DATAQL010000147 DATAQL010000000
VERSION     DATAQL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5359714"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VHS97_02450"
     CDS             <1..564
                     /locus_tag="VHS97_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3447081.1"
                     /translation="LFLGKFFRQGTAIASVAPSSRWLSETTVRNIDWGTVNALVELGA
                     GTGPITRVIARHARPDCRVVVIERDPDFARLLRDRFRAMPNFDIIEGNVRDLDSILAN
                     CGIDRVDHVISGLPVPSFPKELQRSLFEAVRKVLRQDGTYNQITEIPWVFWRFYQGFF
                     DHVQFAFEPRNLPPAGAYYCRGIKMSG"
     gene            584..>2026
                     /locus_tag="VHS97_02455"
     CDS             584..>2026
                     /locus_tag="VHS97_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX3447082.1"
                     /translation="MRDSSRSSGLTGSVPVRGLVNLGFHLHGRLRGQELDHADPVKVQ
                     GETLRRLIKTARLTRFGLDHRFGQIRTVAEFQRAVPIRTYETLWADYLRAAYPVFEDL
                     TWPGRISFLALTSCTTQGATKYIPVSREMVASNRKAAQTVLAFHMGARPDSRLFHGRF
                     FFLGGSTDLKEPAPGVFEGDLSGIAALELSPLMRPFAFPPLDLALETDWDSKLSELAS
                     RSVSERITMVSGVPSWLLMLFQRILEATGKRSLAEVWPHLEMVVHGGVKFDPYREAFE
                     SLLGSPSIRLQETYPCSEGFIAFGDPRTGLLRLIYDHRLFYEFVPVDELSSPNPTRHW
                     LGTAQTGVNYAIVVSTCAGMWAHVIGDTIRFESLSPPMISFTGRTRYTLSAFGEHLIS
                     EEIEAAIAGSAADTGATIRDWHVGPVFKGAMGYHQFVVEFLNEPVDLRRFRDCLDGEL
                     SNRNADYLAHRVPGVGLPPPAVIVAPSGSFE"
BASE COUNT          351 a          592 c          634 g          449 t
ORIGIN      
        1 cttttcctcg gcaagttctt caggcaaggg accgcgatcg cgagcgtcgc cccgagcagc
       61 cggtggcttt cggagacaac ggtcaggaac atcgactggg ggacggtcaa cgccctggtc
      121 gagctggggg caggtactgg cccgatcacg cgagttatcg cccggcacgc ccgcccggac
      181 tgccgggttg tcgtcatcga acgcgacccc gatttcgcgc ggctgctacg ggaccggttt
      241 cgggccatgc ccaacttcga catcatcgag gggaacgtcc gcgacctgga ctcgatccta
      301 gccaactgcg gaatcgatcg agtcgaccac gtcatttcag gactgcccgt cccgtctttt
      361 cccaaggagc tgcaacggtc gctcttcgag gccgtccgca aggtgttacg gcaggatggg
      421 acgtacaatc aaatcaccga gatcccgtgg gtcttctggc ggttttacca gggtttcttt
      481 gaccacgtcc aattcgcatt cgagcctcgc aacctgccgc ctgccggggc ctattattgt
      541 cgcggcatca aaatgagcgg ttagttccac gatggacagg aaaatgcggg atagctcgag
      601 atcttccggc ttaaccggct cggttcccgt acgcgggctc gttaacctcg ggtttcatct
      661 ccatggccgg ctgcggggcc aggagttgga tcacgcggac ccagtgaagg tgcagggtga
      721 gaccctgcgc cggctgatca agacggcaag gttgacccgt tttgggctgg accatcgatt
      781 cggccagatt cgcacggtcg ccgaatttca gcgggccgtg ccgattcgga cttacgagac
      841 gctctgggcc gattacttgc gtgctgcgta cccggtgttc gaagacttga cgtggcccgg
      901 gcgcatttca tttctcgcgc tcacgagctg cacgacccag ggtgcgacca agtacattcc
      961 ggtctcccgc gagatggttg cgtctaaccg caaggccgcg cagacggtgc tggcgtttca
     1021 catgggtgct cggcctgatt cgcggctgtt tcatggtcga tttttctttc tgggagggtc
     1081 aaccgacctg aaagagccgg cgcctggtgt ctttgagggg gacctgagtg gaatcgcggc
     1141 gctcgaactg tcccccctga tgcggccgtt tgccttcccg cccttggacc tggcgcttga
     1201 gaccgattgg gacagcaagc tctcggagct tgcatctcgt agcgtttccg aacggatcac
     1261 catggtgagc ggcgtaccga gttggctgct catgctcttt caacggattc tggaggcgac
     1321 cggcaagcga tcgctggcgg aggtctggcc acatctcgaa atggtggtgc acggcggcgt
     1381 caagtttgat ccgtaccgcg aagcgtttga atcgctgttg ggctcgccgt cgatccgact
     1441 gcaagaaacc tatccatgct ccgaaggatt cattgcgttt ggcgatcccc ggaccgggct
     1501 gctccggttg atttacgatc atcggctatt ctatgaattc gtccctgtcg acgagctcag
     1561 ctctcccaat ccgacgcggc actggctagg cacggcacag accggggtaa actatgcgat
     1621 cgtcgtttct acctgcgcgg ggatgtgggc acatgtgatc ggcgacacca ttcgattcga
     1681 atcgctctcg cctcccatga tcagcttcac gggcaggact cgatacacac tctccgcctt
     1741 tggcgaacat ttgatcagcg aggagatcga ggcggcgata gctgggtccg cggcagacac
     1801 tggtgcgacg attcgagatt ggcacgtcgg tccggtgttc aagggcgcga tgggctatca
     1861 ccagttcgtt gtcgagtttc tcaatgaacc ggtcgatttg cggcggtttc gcgattgcct
     1921 ggacggtgag ctctcaaatc gcaatgccga ttatctggct caccgggttc cgggtgtggg
     1981 gctgccgccg cctgcggtga ttgtggcacc gtcggggagt tttgag
//
LOCUS       DATAQL010000148         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2458959, whole genome shotgun sequence.
ACCESSION   DATAQL010000148 DATAQL010000000
VERSION     DATAQL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2458959"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            734..1252
                     /locus_tag="VHS97_02460"
     CDS             734..1252
                     /locus_tag="VHS97_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3447083.1"
                     /translation="MDIVIRPFREADRPIIRDLTVAGFEGVSIDHNIDERLGPVAGKG
                     WRWRKARDIDRDIDLEGSELAVALDRETGQVAGYITMQCDLETQIGWIHNLVVGSRAR
                     GQGLGRRLLEHALAHFQKAGMKVAKIETLEQNEIGRHLYPSVGFIEVARQIHYAMPLT
                     DPTTDDGAADSR"
     gene            1227..>2591
                     /locus_tag="VHS97_02465"
     CDS             1227..>2591
                     /locus_tag="VHS97_02465"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3447084.1"
                     /translation="MTELPIPVDSTFPALAPRLEMRNITKNFGGVRALRDVSLTARAG
                     EVHAICGENGAGKSTLMKILAGAIIEYDGDVRLDGRTVRFSGPREAEDSGIRIIYQEL
                     NLVPQLTVADNIFLGREKTWGGRLGWLDNRAMEDRSRKLFSRLGTSIDPSAKLGNLRI
                     GDQQMVEIAKTLAFDAAVVIMDEPTSALSDSEVERLFRVIADLRKAGTCVLYISHKMN
                     EVFTLADQVTVLRDGQFVATASRAETRPEQVVRWMVGREIAALNYVQHPIGEQPLLEV
                     LDLCLPTPAGSGRPSLRDISFSVRTGEVLGVAGLLGAGRTELLEAIYGASAFQPAGTI
                     RIEGDEARFTHPDQAIKAGIAMVTEDRKTLGLFDRMTVAENITIRRLPDLTIGPLIDP
                     RAERRAVSWSIKELAIKTAGGGAPVTSLSGGNQQKCILARGLLIEPKVLLLDEPTRGI
                     DVGAK"
BASE COUNT          528 a          774 c          789 g          500 t
ORIGIN      
        1 caagtcggcc cgctcactcg gtggcgacac gctcagtcct tcttcgccgt gagcaccgac
       61 gaagctgggc ggcttccggg tccactcaag accaacaaaa gaaaaacgac tgccaagcag
      121 ccgaagacca cggcatgagt cgttctgtgg tccaggaaag cgacgaaggt cagcgcaccg
      181 gatgcgagca ttcccgtctt aaagatcaac cgtgtcgggc tggtatgcga taaccggccg
      241 gtgacgtgcg tccttaaggg atcggcaatt gataggttat cttcagccga gtctgagatc
      301 tccatgtcgt tctgagcggc tgtcgacccg gtccgtttgc tcgcgggcac gtaatgccct
      361 tcgggttctg gcggaaggga tgccagtccg cagacgggac atcgttgcca ccccgaccaa
      421 ccatcggatc cagacacaag ttccgcgcgg caatgcgggc aagtgtacgt atcaatgaag
      481 tcgatctcgg tcgacacccg actactcccg cttatgggct tgcgcgcctc gtgacgaaaa
      541 agcaagtgtc atcctatgcg gcaatcacgg cggccgcaat tgacctggat acgtccgaga
      601 cctgtccgga cccaggagcg cgacaaccct ctttcgacct ggctgtagcg ggcgtactat
      661 gggcgcgacg ccatggcgag gcggacaggt cgtgccgttt gttgtggctc gcagctgagt
      721 atgacatcac accatggaca ttgtaattcg accatttcga gaggcagata gacccatcat
      781 ccgcgacctg acagtcgctg gctttgaagg cgtttccatc gatcacaaca tcgatgagcg
      841 attgggaccg gtcgccggca aagggtggcg gtggcggaag gcgcgcgaca tcgaccgcga
      901 catcgactta gaggggtccg agctggccgt tgccctcgac agagaaaccg gtcaggtggc
      961 cggctacatc acgatgcagt gcgacctcga gacgcaaatc ggctggatcc ataatctggt
     1021 cgtgggctct cgagcccgtg gccaggggct cggccgcagg ttgctcgagc acgctctggc
     1081 tcattttcaa aaggccggta tgaaggtggc caagatcgaa accctagagc aaaacgagat
     1141 cggccggcac ctttacccgt cggtcggctt catcgaggtt gcgcggcaga ttcattacgc
     1201 aatgcccctg acggacccta ccaccgatga cggagctgcc gattcccgtt gattccactt
     1261 ttcccgccct tgcgccccgg ctcgagatgc ggaacatcac caagaatttc ggtggcgtcc
     1321 gtgcattgcg ggatgtgtcg ttgaccgctc gagctggcga ggttcacgcc atctgcggcg
     1381 aaaacggcgc aggcaagagc actttgatga agatcctcgc tggtgcgatc atcgagtatg
     1441 acggtgacgt ccgcttggac ggccggactg tccgcttttc gggaccgcgc gaggccgagg
     1501 actctggcat caggatcatc tatcaggagc tcaacctcgt tccgcagctc acagtcgctg
     1561 ataacatctt cctgggacgt gaaaaaacat ggggcggcag gctcggttgg cttgacaacc
     1621 gcgccatgga agatcggtcg aggaaactgt tttcccgact tggcacctcg atcgacccca
     1681 gcgcaaaact cggcaatctg cggattggcg atcagcagat ggtcgaaatc gccaagacac
     1741 tggccttcga cgcggcggtc gtgatcatgg atgaaccgac gtcggcgctg tcggattcgg
     1801 aggtcgagcg cctatttcgc gtgatcgccg acttgcgcaa agccggtacc tgcgtactct
     1861 atatcagcca caagatgaat gaagttttca cgttggccga ccaggtgacc gtcctccgcg
     1921 atggtcaatt cgtcgcgacc gcgtcgcggg cggagaccag gccggagcaa gtcgtccgct
     1981 ggatggtagg acgcgagatt gccgccctca attacgtgca acacccgatc ggcgagcaac
     2041 ccttgctcga agttctcgac ctgtgtttgc ccactcccgc cggaagtggc cggcctagct
     2101 tgcgcgatat ttcgttttcg gtccgcacag gcgaggtcct gggagtcgcc ggcttgctcg
     2161 gtgccgggcg aaccgaattg ctggaagcca tctatggagc gagcgccttt caaccggctg
     2221 gtacgatccg aatcgaggga gacgaagccc ggtttacaca tcccgatcag gcgatcaagg
     2281 cggggatcgc catggtcacg gaagaccgca aaactctggg actcttcgat cgcatgaccg
     2341 tggccgagaa tatcacgatt cggcgtcttc ccgacctgac gattggccct ttgatcgatc
     2401 cccgcgccga acgtcgcgcg gtatcttggt cgatcaaaga gctggcgatc aagaccgcgg
     2461 gcggcggcgc gccggtcacg agcctctccg ggggcaacca gcagaagtgc atcctcgcgc
     2521 gcgggctctt gatcgaaccg aaagtcctct tgctcgacga acccacccgc ggtatcgatg
     2581 tcggtgccaa g
//
LOCUS       DATAQL010000149        11286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5054926, whole genome shotgun sequence.
ACCESSION   DATAQL010000149 DATAQL010000000
VERSION     DATAQL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5054926"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..412
                     /locus_tag="VHS97_02470"
     CDS             227..412
                     /locus_tag="VHS97_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447085.1"
                     /translation="MSGPIVRKYGFPNFDKIFGERPLEHGSDDEDASAEPAETANPSE
                     SIKKTSAKKKSSKPKKE"
     gene            complement(504..1466)
                     /locus_tag="VHS97_02475"
     CDS             complement(504..1466)
                     /locus_tag="VHS97_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX3447086.1"
                     /translation="MRRLPTPADAEPSKATGTARLVDPASIVHPAAEPRILGRFRSTD
                     RPAASAEVWVLCILPAVAAAFLLLSLARPARAWGPRGHRASAKLAESRLSPRARAIIR
                     DLLEPGESLADASTWADENSRDIPGSAAWHFVNVPVSANHYDPRDCRPRGCVVSKIAE
                     FRSILADPHTPRSRRRTALLFFVHLVQDLHQPMHVADRNDRGGNNLQLRYGRYDNTNL
                     HQVWDSGLLSQGYRNEAELLRTMDNLAHRPDAGLWLKGHIEDWADESLEVGRRAYLNP
                     RTNTILRTGDSIGRDYERVNVPLVTDRLARSGVRLAFLLNDIFK"
     gene            1650..3899
                     /locus_tag="VHS97_02480"
     CDS             1650..3899
                     /locus_tag="VHS97_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3447087.1"
                     /translation="MALVDPYSPCICGSGQKYKWCCQKVEAYAERSQRLAENGQFEAA
                     LKPLDEGLAKVPDNSWLLTRKALVELQLNQLEPARQTLRTLLHHHPDNLGGMVLLTRL
                     VAEAEGPAEGAAQFQQALSNVRDADRDQLASLAVFVGSALGEAGFSAAALAHLELAGN
                     LQADAKKQSASLVKSLKMNQAISAWEKNPHRLWPVPDRASGPVRESFERALEWAGQGL
                     WSSAASAFELLASGSAADGVADRNRGLCCLWIADNAGAVAALRRYIARAGSTPDAVDL
                     EALCQRVDRIAPYDRVEFVHLTWPIRNRAGLLAALSGNKMFEVGEDRGLDPKDPKSQE
                     VSRFFVLDRPQIEAKAGLARSEIPMVEGELLIGQDTVILETYDDGRLDRLLDRVTAAA
                     GANIPPAHPRTKIIGKEQRHQLALSWRWRIPEGVREEDAERLNDEQVAYIISETWPAT
                     SHPALHRRTPLQAARDGGSEVALRAAVLQLQESPEGWVDLVDWDKLRAKLHLKPEPAI
                     DPSTVDIDELPLSRLTMIPIDRLDDDRVLALYARSREWGIRHVLNRVARLIDSRPSLM
                     VKGKIEAPTLYGELALDAAGRSDRTGALDWIKRGIDSEPPSKRSAHALSWGLIELQVQ
                     MVLDEPEVWVKSLAVILETYRGNNEATSAIFLRLINLGLVQVVPDPNNPDQVALDTRT
                     LEYYLNRFGPRVTTAAGELGVAATRGEIWTPASGAGSAGGKGGAIWTPGSAPAPVPAG
                     GKSKLIIPG"
     gene            4418..6478
                     /locus_tag="VHS97_02485"
     CDS             4418..6478
                     /locus_tag="VHS97_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX3447088.1"
                     /translation="MSTGSELIYLIGSRQNLDDYQKKHWSGSFAEYLDILQRDPRVTR
                     TAYQRIYDMIISHGTDEVVVNKEKTIRYKFFEDPENDGQDAIFGLERTLINLVNILMS
                     AANGYGTERRVLLLHGPVGSSKSTLARLLKKGLERYSRVEEGSLYSFSWKEEGPDGEK
                     VHAPCPMHEEPLHLVPHEHRAEVTAILGKGRGFNDFQVTIEGELCPFCRQMFNERMEL
                     YSGDWARVVDDVVVRRVILSEQDRIGIGTFQPKDEKNQDATELTGDINYRKIAEYGTE
                     SDPRAFNFDGEFNIANRGIIEFIEVLKLDVAFLYDLLGASQEHKIKPKKFAQTDIDEV
                     IIGHTNEPEYKKLQSNEFMEALRDRTVKIDVPYVTRLADEVKIYEKDYNSRKVRGKRL
                     APHTLEVAAMWAVLTRLEEPKNAGLTLMQKLKLYNGKTLPGFTEDNIKELREEAEREG
                     MHGISPRYVQDKISNALVAHPNERSINPFMVLNELEAGLKHHSLINSEETRKHYRHLL
                     SLVKEEYEDIVKNEVQRAIAADEDALTRLCGNYIDNVKAYTQREKVKNKYTGQTEEPD
                     ERLMRSIEEKIDIPESRKDDFRREIMNYIGALSLDGKKFDYRTNERLQKALELKLFQD
                     QKDTIKLTSLVSSVVDKDTQEKIDIVKARLIRNYGYDEDSATDVLNYVASIFARGDVK
                     RGSH"
     gene            6527..7192
                     /locus_tag="VHS97_02490"
     CDS             6527..7192
                     /locus_tag="VHS97_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447089.1"
                     /translation="MKTTEGVGTAVPIYEDLLSRDRKWAMSQGSRYFEEKSAVQDALG
                     RFARHLDNLGIPYAIAGGMALFQHGFRRFTEDVVILVSRDGLNAIHAHLESLGYVPLS
                     TGSKNLRDTEYGVRIEFLIAGEFPGDGKPKAVAFPEPELARVMIACIYYMSLPKLVEF
                     KLASGMTGGIQRMKDLTDVVELIKTLSISAEFADQLNPYVRDKYLELWQGIQDSPVGP
                     AEI"
     gene            7534..8637
                     /locus_tag="VHS97_02495"
     CDS             7534..8637
                     /locus_tag="VHS97_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX3447090.1"
                     /translation="MVRKIDHDANRFKQIVRGKIKSDLRKYITHGEMIGKTGGEFVSI
                     PLPQIEIPEFRYGTKNRGGVGQGPGDVGTTIGRSSDGDGGAGAGDAPGHHILEVELTM
                     DDLAAILGEELALPRIAPKGRANIIEEKDRYTGIRQTGPESLRHFKRTYKKALKRQIA
                     SNTYDPREPLVIPIREDKLYRSWNPIALPQFNAIVFYLMDVSGSMTDDQKEIVRAEAF
                     WIDAWLKSQYDGVTTRYIIHDAVAREVDEHTFYHTRESGGTRISSAYNLANKIIDDQH
                     PQADWNIYVFHFSDGDNWGEDNRQCIGLLRDQLLPKCNLFCYGQVESPYGSGEFYREL
                     EEAFEDEDNLALSEIRNKEGIYDSIKEFLGRGR"
     gene            8720..10285
                     /locus_tag="VHS97_02500"
     CDS             8720..10285
                     /locus_tag="VHS97_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX3447091.1"
                     /translation="MSTVNTHNLPDDLRAIQLETEAHARDYGLDFYDTIFEVLDYDEL
                     SEIAALGGFPNRYPHWRFGMEYQQLAKGYRYGLQKIYEMVINNDPCYAYLLRCNQLVD
                     QKLVMAHVYGHNDFFKNNIWFSQTNRKMMDEMANHGNRIRSCMERHGEETVENFIDSC
                     LSLENLIDIYSPLIKRREEINRYDFVGDQEDDEANAPTKFQSKGYMDAYVNPPRVLKE
                     EARKRETEREKERAKPSSIPEQPERDVLLFLVEHAPLKAWQHDVLAIIREEAYYFAPQ
                     GQTKIMNEGWASFWHSTIMTQKTLHPSELVDYADHHSGTMATQPGRLNPYKLGIELLR
                     DIEERWNKGQFGPEWDECDNLDARRKWDKKLGLGRQKIFEVRRIHNDVTFIDTFLTVD
                     FCRRYKLFSYRHNDQTETYEIESREFKKIKERLLFNLTNFGQPIIRVKDANHRNRSEL
                     YMQHEYFGVDLRIDHAQDTLRHLHRLWTRPVHLETCVDGRPTLLSFDGTDHSIRPLGG
                     SSHESEQKDRRRA"
     gene            10251..10736
                     /locus_tag="VHS97_02505"
     CDS             10251..10736
                     /locus_tag="VHS97_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447092.1"
                     /translation="MSLSKKIAAALDENTKAYVLPCTVTIEDSPNRLTLHLSSLDTVG
                     LAFSTMEYANTSRTELSSDALKEWADRLTKRVTYLMEPLKVVEVDDRGGEVQVRSQSP
                     TPRADERGYYEMRLFRQGNLRMERFVFDGGARQRRPTDCRLTREVLERLADDIVASAG
                     A"
BASE COUNT         2563 a         3281 c         3349 g         2093 t
ORIGIN      
        1 gaaacagaca caaaatcaat aaactactaa ctaccgaaat taaaccgcgc cgcgcgatcc
       61 tcaccccctg attttccgga aaaaccaggg tgcagattct ggatacggga ctggacgtat
      121 ccccatgtat aggttaaaat acatggtaaa taacgattga gtaagctggc aatcagccgc
      181 gatcggccca aatgcctgtg acgtcattac ctgggagaca gccaccatga gcggtccgat
      241 cgtccggaag tatggcttcc cgaattttga caagattttc ggcgagcgcc ccctcgagca
      301 tggcagtgac gacgaggatg cgtcagccga gccggcggaa acggcgaacc cttccgaatc
      361 aatcaagaaa acatcggcca agaagaagag ctcgaaaccg aaaaaagagt agaagccgat
      421 cgacagatgg tgcggagagc atctggccga caaacttcac aagcggcgcg aaacgccggg
      481 cgttgcacat tctcggagag agctcacttg aaaatatcat tgagaagaaa cgcgagtcta
      541 actcccgagc gagccagccg gtcggtcaca agtggaacgt tgacccgctc gtagtcgcgg
      601 ccgatcgagt cgccggtgcg caagatggtg tttgtccgcg gattcaagta ggcgcgtcgt
      661 ccgacttcga ggctttcgtc ggcccagtct tcgatgtggc ctttgagcca gaggcccgcg
      721 tcaggccgat gggcgagatt gtccatagtg cgcaagagct cggcctcatt gcggtagccc
      781 tggctgagta atcccgaatc ccagacctgg tgtaaattgg tgttgtcgta gcgtccgtag
      841 cggagctgga ggttgttgcc cccgcggtcg ttccgatccg cgacgtgcat cggctgatgg
      901 aggtcctgaa ccagatggac aaagaaaaga agagctgtcc gccgccgcga tcgtggggtg
      961 tggggatcgg caaggatcga ccgaaattcg gcgattttgg agacgacgca cccgcgcggc
     1021 ctgcagtcgc gggggtcgta atgattggcc gagaccggca cgttgacgaa atgccaggcg
     1081 gcactgccgg gaatgtcgcg gctgttttcg tcggcccaag tcgaggcatc cgccagcgat
     1141 tcaccgggct cgagcaggtc gcgaatgatc gccctggctc gcggggacag tcgcgattcg
     1201 gcaagcttgg cagaagctcg atggccgcgg ggaccccatg cccgagccgg tcgagccagg
     1261 ctgaggagca agaaagcggc agcaaccgcc ggcagtatgc acaataccca aacttccgcg
     1321 gacgccgcag gtcgatccgt cgagcggaat cgccccagga tccttggttc ggctgcggga
     1381 tgcacgatcg acgcgggatc gaccaaacgg gcggttcccg ttgctttgct tggctcggcg
     1441 tcagctgggg tggggagacg tctcatcatc gtcgcggcgg ggattaaagc ggaagttaga
     1501 gcggcccaac cgggccacac gcacaccagc gtcagttgcg tctcgatcct ggttcgcagt
     1561 ggcatcagca tagacggccc gcgcgggctt ttgctagtgt gtcgaggaac ggattagagc
     1621 gcgacccgac ttttctagaa aggcccacga tggcgttggt cgacccttat tcgccttgca
     1681 tctgcgggag cggacagaag tacaaatggt gctgccagaa ggtcgaagcc tacgccgaac
     1741 gttcccagcg gctcgcggag aacggccagt tcgaggcggc cttgaagccc ctggatgaag
     1801 ggctcgccaa ggtacctgac aattcctggc tcctgacgcg caaggctttg gtcgagcttc
     1861 agctcaacca gcttgaacca gccaggcaga cgttgcgcac gctcctgcac catcatcccg
     1921 acaacctggg tgggatggtg ctcttgaccc ggctggtcgc ggaggccgag ggtcctgccg
     1981 agggggcggc ccagttccag caagccttgt cgaacgtccg cgatgccgac cgtgaccagc
     2041 ttgcatcgct ggcggtgttc gtcggttcgg cgctcggaga ggcaggattc agcgccgcgg
     2101 cgctagcgca tctcgagctc gccggtaacc tccaggccga cgccaagaag cagagtgcct
     2161 cgctggtcaa aagcctcaag atgaaccagg cgatttccgc ctgggagaag aatccccatc
     2221 gcctctggcc ggttcccgac cgtgcctcag gtcccgtccg agaatcgttc gaacgcgcgc
     2281 tggaatgggc cggacagggg ctctggtcgt ccgcggcgtc ggccttcgag ctgctggcat
     2341 cgggctccgc ggcggacgga gtcgccgacc gcaatcgggg gctgtgttgc ctctggattg
     2401 ccgacaacgc cggcgccgtg gccgcactac gacgttacat cgcgcgcgcc ggatccacgc
     2461 ccgacgccgt ggatctcgag gcactttgcc agcgggtcga ccggatcgcg ccttacgatc
     2521 gagtcgaatt cgttcaccta acctggccaa tccgtaaccg agccggcctc ttggccgcgc
     2581 tctcgggcaa caagatgttc gaggtggggg aggatcgagg tctcgatccc aaggatccca
     2641 aatctcaaga ggtctcacgg tttttcgtgc tcgatcgacc ccagatcgag gcgaaagccg
     2701 gactcgcgcg tagcgagatc ccgatggtcg agggagagct cttgatcggt caggacacag
     2761 tcatccttga aacgtacgac gacgggcggc tcgaccggct gctcgatcgg gtcaccgcgg
     2821 cggcaggcgc gaatatcccg ccagcccatc cccgaaccaa gatcatcggc aaggaacaac
     2881 gtcaccagtt ggcactaagc tggcgctggc gaattcccga aggggtaagg gaagaggatg
     2941 ccgagcgtct caacgacgag caagtcgcgt atatcatcag cgagacctgg ccggcgacat
     3001 cccacccggc actgcaccgc cggactccgc tgcaggccgc ccgggacggc ggttccgagg
     3061 tcgcgctgcg ggcggccgtt cttcagctgc aagaatctcc cgaaggctgg gtcgatctgg
     3121 tcgactggga caagctgcgc gccaagctcc atttgaagcc agagcctgcc atcgatccca
     3181 gcacggtgga catcgacgaa ctgccgctct ctcgattgac gatgattccg atcgaccgct
     3241 tggacgatga ccgcgttctg gccctttacg cccggtcacg cgagtgggga attcgccacg
     3301 ttctaaaccg ggtggcccgg ctcatcgaca gccgcccatc cctgatggta aaaggcaaaa
     3361 tagaagcgcc caccctctac ggagagttag cacttgacgc tgccggacgg agcgaccgta
     3421 ccggagcgct cgattggatc aagcgcggaa tcgactccga acccccctcc aagcgctcgg
     3481 cgcacgcact gagctgggga ttgatcgagc tgcaggtgca gatggttctc gatgagccgg
     3541 aggtttgggt caagagtctc gcggtgattc tggaaactta tcgaggcaat aacgaagcga
     3601 catcggccat attcctccgc ttgatcaatc tcggcttggt tcaagttgtg ccggatccga
     3661 ataatcccga tcaagtggcg ctcgacaccc gcacgcttga atattacctc aatcgcttcg
     3721 ggccgcgggt taccacggcc gcgggggaac tgggtgtcgc tgcgacacgt ggcgagatct
     3781 ggactccggc tagcggtgcc ggcagcgcgg ggggtaaggg cggtgcgatc tggacgccgg
     3841 gctccgcccc tgccccggtg ccggcaggag gaaaatcaaa gctcatcatt cccggatgat
     3901 gaccaagtca gccgaccgac gcctgcgagg agatcggaaa cgctggaatc gccggtgacg
     3961 gtgcagtatc gatgggactg ttcgagggcg gttgaaggtc tatgaaaagg gtgcggcctt
     4021 gcccgttctc aaacgctggt ctcacattgg atcgcttgac ataaaagaga tctcggagtt
     4081 gtaataaacg tggtgcggac gaatctgaaa ccgaagcgcg ctcccgtccg tgatgtatta
     4141 gcgagcgtgg agagcacttc gtccgttccc ttggacctat tgacttccaa tcggccacat
     4201 cggccgctga cttattcggg cattccccat cgttgacgag gcgcgttgac aatttgttgg
     4261 ccgagccctc acacggaatt aatgaggatg caaaaaccct gcgtgcgggc gttggtccgg
     4321 ctcgggggtg gcccgatggg tagggccttg tcagttttca tcttgtgaat ggccccggca
     4381 aagcctgatg gagccgccct caggagggaa acgtctcatg tccaccggat cagagttgat
     4441 ctacctgatc ggcagtcgtc agaacctcga cgactatcaa aaaaaacact ggtcggggtc
     4501 gttcgccgag tacctcgaca tcttgcagcg cgatcccagg gtcacccgga cggcctacca
     4561 gcggatctac gacatgatca tcagccacgg aaccgacgag gtcgtggtca acaaggaaaa
     4621 gacaatccgg tacaagtttt tcgaggatcc ggaaaacgac ggccaagacg cgatcttcgg
     4681 cctcgaacgg acgttgatca atctggtcaa catcctgatg agtgccgcca acggctatgg
     4741 gaccgagcgg cgagtattgc tgctgcacgg tccggtcggc agctcgaaga gcacactggc
     4801 ccggctgctg aaaaaagggt tggaacggta ctcgcgcgtc gaagagggaa gtctatacag
     4861 tttcagctgg aaggaagaag ggcccgacgg cgaaaaagtt catgctccct gtccgatgca
     4921 cgaagaaccg cttcatcttg tcccgcacga gcatcgcgcc gaggtcacgg caattctggg
     4981 caaagggcga ggtttcaacg actttcaggt cactattgaa ggagaactct gcccgttctg
     5041 ccgccagatg ttcaatgagc ggatggaact gtactccggc gattgggccc gcgtggtcga
     5101 cgacgtcgtc gtacggcgag tcatactttc cgagcaggat cgcatcggca tcggcacgtt
     5161 ccagcccaag gacgaaaaga accaggacgc caccgagcta accggcgaca tcaactatcg
     5221 caagatcgcg gaatatggca ctgagagcga cccccgcgca ttcaatttcg acggcgagtt
     5281 caatatcgcc aatcgcggca tcatcgagtt catcgaagtc ctcaagctcg acgtcgcctt
     5341 cctctacgat cttttgggcg ccagccagga acacaagatc aagcccaaga agttcgcgca
     5401 gaccgacatc gatgaggtga tcatcggtca caccaacgaa ccggaataca agaaactgca
     5461 atccaacgag ttcatggaag cgctgcgcga tcgtaccgtc aagatcgacg tcccctatgt
     5521 gacgcggctc gccgacgagg tcaagattta cgagaaggac tacaacagcc ggaaggtccg
     5581 cggcaagcgc ctggcgccgc atacgctcga ggtcgccgca atgtgggccg tcttgacacg
     5641 gctcgaggaa cccaagaacg ccgggctgac cttgatgcaa aagctcaagc tctacaacgg
     5701 caagacgctt cccggtttca cggaagacaa catcaaagag ctgcgcgagg aggccgaacg
     5761 cgaaggaatg cacggtatca gtccgcgcta tgtgcaggac aagatatcca acgccttggt
     5821 cgctcatccc aacgagcggt cgatcaatcc gttcatggtc ttgaacgagc tggaagccgg
     5881 actcaagcac cactcgctca tcaacagcga ggaaacgcgc aagcattacc ggcacttgct
     5941 gtcgctcgtc aaggaagaat acgaggacat cgtgaaaaac gaggttcaac gcgccatcgc
     6001 cgccgacgaa gatgctctta cgcgtctctg cggcaactac atcgacaacg tcaaggccta
     6061 cacccagcgc gagaaggtca agaacaagta caccggccag acggaagagc cggatgagcg
     6121 gttgatgcgg tcgatcgaag agaaaatcga tattccggaa agccgcaaag acgacttccg
     6181 ccgcgagatc atgaactaca tcggcgcgct gtccttagac ggtaagaagt ttgactaccg
     6241 gaccaacgag cggctgcaga aggcccttga gctcaagctg ttccaggacc agaaagacac
     6301 gatcaagctt accagcctgg tatcaagcgt cgtcgacaag gacacccagg aaaaaatcga
     6361 catcgtcaag gcccggttga ttcgtaacta tggctacgac gaagactcgg ccaccgacgt
     6421 ccttaactac gtcgccagca tcttcgctcg cggggacgtc aagcggggga gccattgatc
     6481 gagcacgtac ccaccttcgg ccgacctctc tttgggagaa actccgatga aaacgaccga
     6541 aggtgtcggg acggccgtgc ctatctacga agatctgctg agccgggatc ggaaatgggc
     6601 catgagccaa ggaagtcggt atttcgagga gaaaagcgct gtgcaggatg cactggggcg
     6661 cttcgcacgg catttggaca atctcggaat tccgtacgcg atcgctggcg gaatggcgct
     6721 attccagcat ggtttccgtc ggttcactga agacgtcgtt atcctcgtat cgcgcgatgg
     6781 cctcaatgcc atccacgccc atttggaaag tctgggatac gttccccttt ccaccgggag
     6841 caagaatctc cgcgacacgg agtacggcgt ccgcatcgag ttcttgattg caggcgagtt
     6901 tcccggcgac ggcaaaccca aggcggtggc tttccccgag ccggaactgg cccgagtgat
     6961 gatcgcttgt atctactaca tgtcgctgcc aaagcttgtc gaattcaagt tggcgtcggg
     7021 gatgacgggt gggattcagc gaatgaagga tttgaccgac gtcgtggagc tgatcaaaac
     7081 gctttcaatt tccgccgaat ttgccgatca gctaaacccg tacgtccgtg acaagtacct
     7141 cgaactatgg cagggaatcc aagattcacc tgtcgggccg gccgaaatct gaacccagga
     7201 cgcctgccgc ggtgtggcgt aaaccgaaca ttcggcagcg aaatcgaaat ccaccatgca
     7261 tttggcttgg acaccggatg gctcaaggaa tacaatttgc aagggacata gaatggggtc
     7321 aatgagtttg gcgaccggac agagataacg ccagaccgtc ctcgacccac gcctacccgt
     7381 cgcttcaggc agttttcccc taattgatct tttgtttttc gactcggtgg accgctgagg
     7441 ccgcggcgca ttttgctgcg cgtttgggca cgaggtggtc taaattgggg cgtgaccgcc
     7501 gtcccgatcc ccagaggcga ggattataaa gccgtggttc gaaaaatcga tcatgatgcc
     7561 aatcggttca agcagatcgt ccgcggcaag atcaaatcgg acctgcgtaa gtacatcacg
     7621 cacggcgaga tgatcggcaa gaccggcggc gagtttgtat cgatcccctt gccacaaatc
     7681 gagatccccg agtttcgcta cgggaccaaa aaccgtggcg gagtaggcca agggcccggc
     7741 gacgtcggca ccacgatcgg ccgatccagc gatggcgatg gcggtgccgg cgcgggcgat
     7801 gcgcctgggc accacatcct cgaggtcgag ctgaccatgg atgatctggc cgccatcctg
     7861 ggcgaagagc tggcgctgcc gagaattgcc cccaaggggc gagccaatat catcgaagaa
     7921 aaagacaggt acaccggcat tcgccagacc ggcccggaga gtctgcggca cttcaagcgc
     7981 acctacaaga aagccctcaa gcgccagatc gcttccaaca cctacgaccc ccgtgagccg
     8041 ctcgtcattc cgattcgcga ggataagctc taccgttcgt ggaacccgat cgcactaccc
     8101 cagtttaacg ccatcgtctt ctacttgatg gacgtctcgg gctcgatgac cgacgaccag
     8161 aaagaaatcg tgcgggccga ggccttttgg atcgacgcct ggctcaagag tcagtacgac
     8221 ggcgtgacca cccggtacat cattcacgac gcggtggccc gcgaagtcga cgagcacacc
     8281 ttctatcaca cacgcgagag cggcggaacc aggatcagct cggcctataa cctcgccaac
     8341 aagatcatcg acgaccagca tccccaggct gactggaata tctacgtgtt ccatttttcc
     8401 gacggagaca actggggcga agacaaccgg caatgcatcg ggcttttgcg cgaccagctt
     8461 cttcccaagt gcaacctgtt ctgctacggc caggtcgaga gcccgtatgg ctcgggcgag
     8521 ttctaccgcg agctggaaga ggcgtttgaa gacgaagaca acctggcgct gtcggagatt
     8581 cgcaacaaag aaggtattta cgactcgatc aaagagttcc tcggtcgcgg gcggtgaatc
     8641 caaagaccca acaatctaag actggcgaac gtaccaagag gtagatcccg atccatcccc
     8701 ggttcgcgag acgaagtcca tgtcgacggt caatactcac aatctgccag atgacctccg
     8761 tgcgatccag ctcgagaccg aagcgcacgc gcgcgattac ggcctggact tctacgacac
     8821 gattttcgag gtgctcgatt acgacgaact ctcagagatc gccgctctgg gcggttttcc
     8881 caaccgctat ccgcactggc ggtttgggat ggagtaccaa cagctcgcca aaggttatcg
     8941 ttatggactc caaaagattt acgaaatggt catcaataac gacccgtgct acgcctacct
     9001 cctgaggtgc aaccaactgg ttgaccaaaa gctggtcatg gcccacgtct acgggcacaa
     9061 cgatttcttc aaaaacaaca tctggtttag ccagaccaac cgcaaaatga tggacgagat
     9121 ggccaaccat ggcaatcgaa tccgctcgtg catggagcgc cacggtgaag agactgtcga
     9181 gaacttcatc gatagctgct tgtcgctcga gaacttgatt gacatctact cccccttgat
     9241 caagcgccgc gaggagatca atcggtatga tttcgtgggc gaccaggaag acgacgaagc
     9301 gaatgccccc acgaaattcc agagcaaggg ttatatggat gcgtatgtga atccgcctcg
     9361 ggttctcaag gaggaggctc gcaagcgaga aacagagcgc gaaaaagaga gggccaagcc
     9421 gtcgtcgatt cccgagcagc cggaacgtga cgtgctgctc ttcttggtcg agcatgcgcc
     9481 cttgaaggcc tggcagcacg acgtcctggc gatcatccgc gaagaagcgt attacttcgc
     9541 gcctcagggc cagaccaaga tcatgaacga gggctgggcc tcgttctggc actcgacgat
     9601 catgacccag aagacactcc acccgtcgga gctggtcgac tatgccgacc accactccgg
     9661 gacgatggcc actcagccag gccggctcaa tccgtacaag cttggcatcg agctcttgcg
     9721 cgacatcgag gagcggtgga acaaagggca attcggcccg gaatgggatg aatgcgataa
     9781 tctcgatgcg cggcgaaagt gggacaaaaa gctgggcttg gggcggcaaa agatttttga
     9841 agtgcggcgc atccacaacg acgtgacatt tatcgacacc tttctgacag tggacttttg
     9901 ccgccggtac aaactcttta gctaccggca caacgatcag accgagacct acgaaatcga
     9961 gagccgcgag ttcaagaaga tcaaggagcg actacttttc aacctgacca atttcgggca
    10021 gcctatcatc cgcgtcaagg atgccaatca ccgcaatcgc tctgaactct acatgcagca
    10081 cgagtatttc ggggtcgatc ttcggatcga ccacgcccag gacacgctcc gtcatctgca
    10141 ccgtctctgg acccgtccgg ttcacttgga gacctgcgtt gatgggcggc ccacattgtt
    10201 gtcgtttgat ggtacagatc actcgatccg tccgttggga ggttcttccc atgagtctga
    10261 gcaaaaagat cgccgccgcg cttgacgaaa ataccaaggc ttatgtgctt ccctgcactg
    10321 tcacgataga ggatagcccc aatcgcctga cgctccacct ttcatcgctc gatacggttg
    10381 ggctggcctt ctccaccatg gagtacgcca acacgagccg gaccgagcta tcctcagacg
    10441 cgcttaagga gtgggcggat cgcctcacca agcgggttac gtacttgatg gaaccgctca
    10501 aggttgttga ggtcgacgac cgggggggcg aagtccaggt ccgcagccag agtccgaccc
    10561 ctcgtgccga tgaacgtggc tattacgaga tgcgactgtt ccggcaagga aacttgcgca
    10621 tggaacgatt cgtgttcgac gggggcgctc gtcagcgccg tccgaccgat tgccggctta
    10681 cgcgcgaggt tctggaacgg ctggcggatg atattgtcgc cagcgccggc gcgtaacctt
    10741 gggctaacta agaaagtcca cgacgaacga atggcaccgc gtagcaatat tttcgttggt
    10801 cttcagataa cccagggcgt tgccctgggc tgatttgtta atacccccat tggggcaaaa
    10861 aggtaatggt gccgcccgca gggatctcgc cccgaagggg caataacgtg ccagcccagg
    10921 gcaacgccct gggaggccga ccaacggcaa aagccttgcc cttcaaatgc cgaacatggg
    10981 gaatcatccg gcggagcctg cgggtgagtt ccggtgaatc ggatgggcag gtcgccggac
    11041 cgccgagctt ggacgcggta ttggccggat cgatggcgga cggtctcatc tccggcttat
    11101 gagcatagat cgaccgccaa gcgagagcgg gagaattcgg ccgctcttcg atcgaactta
    11161 ggctaaagcg gcagccacca tcggtctccg tctcgtctcc gttcgatatt ctcgatgagt
    11221 ctgcgaacct ctgccagctc tgcggccgac gccttctcgg ttgcgagcgc ctgcatgact
    11281 aacttc
//
LOCUS       DATAQL010000150         9255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3374691, whole genome shotgun sequence.
ACCESSION   DATAQL010000150 DATAQL010000000
VERSION     DATAQL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3374691"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VHS97_02510"
     CDS             complement(<1..1118)
                     /locus_tag="VHS97_02510"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX3447093.1"
                     /translation="MATLLAITVLLPLVGSLVLVLTPRLEAQRARSIGLSFALVTLAL
                     SLVMLLAFRAEVSGPQFAFMAADGRYGLGWIERPDIRFALGLDGISLWLFILTSLLVM
                     TAFGASWLSITERVPLYYAFILALETGLLGLFASLDVVLFYIFFEFTLIPLFFLIGLW
                     GGPQRRRAAVTFFLYTLAGSLLTLLGVIALVVVHFQFSKTNLLTFSIPELTQGLAALP
                     WDEWRDTSSWTSPQVVIFLLLLAGFAIKVPMFPFHTWLPLAHVEAPTAGSILLAGVLL
                     KVGTYGLLRFNIGMTPLGATALFPLLSTLAVIGIIYGALAALAQTDIKRLVAYSSVSH
                     MGFIVLGIFAFNATALDGSAIQMVNHGLTTGALFACVG"
     gene            complement(1149..3203)
                     /gene="nuoL"
                     /locus_tag="VHS97_02515"
     CDS             complement(1149..3203)
                     /gene="nuoL"
                     /locus_tag="VHS97_02515"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX3447094.1"
                     /translation="MSDSFVSIAWLIPLSPLMGAGLAAFGPKPVRAVAHIPVIAGIAL
                     AFLLSAGLLFAADPGKTTLVMRWLSISNLEVPIEFRIDGLTTMMLAMVTFVSSLVAVF
                     AAGYMAGDPSYPRFFALIGLFVFSMTGLVLSNNYVLTYAFWEGVGVCSYLLIGFWHTR
                     PSATAAAIKAFLVNRIGDVGFAIAIFWLWSIVPNHDLSYGNVLAESTLRGMPPGAVVG
                     IGLLLFWAATAKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYLIARSMPLLTL
                     APGVLLLIASIGCATALLSAAIAVTQTDLKRVLAYSTVSQLGYMFMALGSGVSNVAQL
                     AIVAAMFHLFTHAFFKALLFLAAGSVMHAMGDVIDMRRFRGLRHRLPHTFRTFAVGGL
                     ALSGIFPLSGFFSKDEILLSLKLAAHTGGWIYLLIYWLAVFTAFLTAFYTGRAFFMTF
                     WGIEKLPSPDDPEAPELASAVDSHALHGDLGAGHGHEDDSHAHGHGSHVGHESPPIMT
                     YPLYALAACTILIGLVCLLSGLFGGGTAEWFGHHLHATLGFESLGHEVHHFDWLIAIT
                     GTVAGVGGLALAYLMYAEPSPIPDRLSQRLAPLYEASLHKFRVDELYEWLFVRPTRAL
                     AVVCEFLDTYLVDRLVRGVAMIPPTVGRDMLARYQNGLIQFYATISALSVAILLLILL
                     LI"
     gene            complement(3351..3827)
                     /gene="nuoK"
                     /locus_tag="VHS97_02520"
     CDS             complement(3351..3827)
                     /gene="nuoK"
                     /locus_tag="VHS97_02520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX3447095.1"
                     /translation="MAPTDEFTLDILTNYLLVGAALFALGMLGFLARRNLIVMFLSVE
                     MMLQGIALSLVGFGRFHGSWTGQIFTIVILTVAACEASVALALIVVLYNQRSSLDVTL
                     WQDIREAVVPASSPIAEETLDAEPVQPTLGPESYPHLVPAGIEPPHPTQPWIERRN"
     gene            complement(3878..4705)
                     /locus_tag="VHS97_02525"
     CDS             complement(3878..4705)
                     /locus_tag="VHS97_02525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX3447096.1"
                     /translation="MTIGDAILATLVVVLGAAGTYLLLPHQHGGARPRRVHGAGAISA
                     SLGLLAFLLFWSPPGPFVTTVFFYIFSLAATIGALLTVTSRNPVYSALWFASVVLATS
                     GLFLLAGAPFLAAGTVIVYAGAIIVTFLFVIMLAQVEGKALYDRASRAPGRATFTCFL
                     LLWSLIYSLSTLRTHPHDGGPTPIQSGSLKRTREIPQVFSLQPMGEHLAVLASAYRPT
                     SWIYDTKGKEKLNVAGLGEALYTDHLVTAGLTGVLLFVALVGAVAIANPKKTERAGS"
     gene            complement(4771..5352)
                     /locus_tag="VHS97_02530"
     CDS             complement(4771..5352)
                     /locus_tag="VHS97_02530"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX3447097.1"
                     /translation="MRADDPKLLRLEPPQLKAWEKFFLPQIAVGLGVTARHIAGVMFA
                     NKAITVQYPEEQHVPSPNYRGVHRLNKDEEGRVKCVACMLCATACPAHCIDIVGATAP
                     STWPDREKYPESFVIDELRCIYCGMCEEACPVEAIELTGLYDLTGLSREEMIFDKVKL
                     LSVFDTTKSHEPMRYGKPPAEQSSATTILPSLE"
     gene            complement(5408..6439)
                     /gene="nuoH"
                     /locus_tag="VHS97_02535"
     CDS             complement(5408..6439)
                     /gene="nuoH"
                     /locus_tag="VHS97_02535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX3447098.1"
                     /translation="MWLLIGVLLKILIVTNVLLGAVAYLIYVERKIAAYAQDRIGPNR
                     AGGEFGVPFGLLQPLADGAKMLLKEGVIPGYVNKPLYLLAPMIAIVATLIAFAVVPFG
                     PVGLGQTIDFQIAPGVDVGIVYIFAVGSLAVYGVILAGWASNNKYSFIGGLRSSAQLI
                     SYEVPLGLSILGMVLWAGSLDLSTIINWQDRHVWGVIASPIGFLIFLVSAFAETNRLP
                     FDLPESEQELVGGFHTEYSGMKFGMFFLGEYVHVITVSFLTVILFFGGWDFPLLSQEQ
                     TGLLSAVAKLMVILIKVALVILFMMWVRWTLPRFRYDQLMDLAWKSLIPLALVNLVAT
                     AVIVQMLRS"
     gene            complement(6521..8269)
                     /locus_tag="VHS97_02540"
     CDS             complement(6521..8269)
                     /locus_tag="VHS97_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3447099.1"
                     /translation="MATIIINSNEYTIPEGEKLNAIQMAERVGIDIPYYCWHPALSVV
                     ANCRMCEIEVGTKDAKTGEIKLLPKLVPGCQTPAKDLTVLVTNSPKVKEHQRMIMEYL
                     LLNHPLDCPVCDQAGECGLQDYSYRYGQSVHRFVEERTVNPRKDVSDLIQLNMDRCIM
                     CTRCVRFTREISQTGELEVMRRGSHAEIDVFPGEPVDNPLAGNIVDLCPVGALLDKDF
                     LHKQRAWFLSKHDSICTRCSTGCNISSEENRGALWRFKPRHNPLVNDYWICDEGRYSY
                     KAANDPNLLAAMYVRKNNDLQPVAFGEAIQAVDRGFKEIGQRGGLVAAVLSPFLTVEE
                     AYLLARYVKGLNPGNVLALGPVPAHGTDQTFQPDQTKGRTGDTSFVVPRPFTIHAEKC
                     PNRQGVAAVLEHFEGKVIAFDELAKRVGAGELSGLYVVSDAIEPWIDLDQSNSLRAKV
                     SFLVVQDTTVTPLAHLADVVMAGATFAEKAGSYVNASGRLQYSEAALPPRDGSLPDLD
                     ILGILLDRAGGPVCSSEILAELAETIPAFGLAKGGKLPPYGVVIGKSPPPATKAPVPP
                     SFNDAWFVAHGAARWR"
     gene            complement(8362..>9255)
                     /locus_tag="VHS97_02545"
     CDS             complement(8362..>9255)
                     /locus_tag="VHS97_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX3447100.1"
                     /translation="GLIESLEGKRGWPRIKPPFPAIEGAFRKPTVVNNVETLCCVPHI
                     IERGASWFKSIGTPKSYGPKLYCVSGHVNKQVCVELALGVTCRTLIDEHAGGVWKGRK
                     AKAAVPGGISMGLLSAAELDTPLDFESLRKPGCLGLGTAAVTVVDDQTSIVDYLYNTC
                     RFFAHESCGQCTPCREGTTWFYKTIKRIKFGGGRSEDLDILLNLADNMGIIPGTTICG
                     LADGAAWPIKNAVAKFRGELEDYIRTHQSPAAQVKPLQEKIAHGIPADLDLAAGRLTV
                     LSNTGNGRASLALHQPRPEPA"
BASE COUNT         2068 a         2783 c         2780 g         1624 t
ORIGIN      
        1 atccccacgc aggcgaacag agcccccgtc gtgagcccat ggttgaccat ctggatcgcg
       61 gatccgtcga gggcggtagc gttgaacgcg aagattccga ggacgatgaa acccatgtgg
      121 ctcaccgagc tgtacgcgac cagccgcttg atgtcagtct gagctagagc cgctaaagcg
      181 ccataaatga tgccaatcac agccagagta gataacagcg ggaagagcgc ggtcgccccc
      241 aatggggtca tgccaatgtt gaatctcaag agaccatagg tccctacttt gagaagaacc
      301 ccagcgagga ggatagagcc ggccgtcgga gcttcgacgt gggccagcgg cagccaggta
      361 tggaagggaa acattggcac cttgatcgca aagcccgcca acaggagcag gaaaatcaca
      421 acctgcgggc tggtccacga cgacgtgtcg cgccattcat cccacggcaa cgccgccagg
      481 ccttgcgtca gctcgggaat cgaaaacgtg agcaggttgg tctttgaaaa ctgaaagtgc
      541 acgaccacca gtgcgatcac gcccaggagc gtgagcaggc tgccggcgag cgtgtacaga
      601 aaaaaagtca ccgccgcgcg ccgtcgctgc ggcccgcccc acaaaccgat caggaaaaaa
      661 agcgggatca gcgtgaattc gaagaagatg taaaacagca cgacgtccag gctggcaaac
      721 aagcccagca agcctgtctc aagcgccagg ataaacgcgt aataaagcgg cacgcgttcc
      781 gttatcgaca gccaggaagc cccaaacgcc gtcatgacca agagcgatgt caatataaag
      841 agccagagcg agatgccgtc gagtcccagg gcaaagcgaa tgtcggggcg ctcgatccag
      901 cccaagccgt agcggccgtc cgccgccatg aacgcaaatt gggggccact cacctccgcg
      961 cggaacgcca acagcatgac caggctcagg gccagcgtca caagcgcgaa cgaaagtccg
     1021 atcgatcggg ctctttgtgc ttcgagtctt ggcgtcaaga cgagaaccaa gcttcccacg
     1081 agagggagca ggaccgtgat cgccagcaat gtcgccatcc gtctatcctc gaggcctgga
     1141 accgggaatc aaatcaataa caagatcaac agcaggatcg ccaccgagag cgccgagatg
     1201 gtcgcgtaaa actggatcag tccgttctga taccgcgcga gcatgtcccg gccgactgtc
     1261 ggcgggatca ttgccacccc tctcaccagc cgatccacca gataggtgtc aaggaattcg
     1321 catacgaccg ccagtgcgcg cgtcggccgg acaaagagcc actcgtaaag ctcgtcgacc
     1381 cgaaacttgt gcagcgatgc ctcatagagt ggcgcgagcc gctgactcaa tcggtcgggt
     1441 atcgggctgg gctctgcgta catcagatag gcgagtgcca gaccgcccac gccggcgacc
     1501 gtgcccgtaa tcgcaatcag ccagtcgaaa tgatgcacct cgtgtccgag cgattcgaag
     1561 ccgagagtgg cgtgcagatg atggccgaac cattcggccg tgcccccgcc gaacaggcca
     1621 gaaagcaggc ataccagacc aatcaagatc gtgcaggcgg ccagggcgta gagcgggtaa
     1681 gtcatgatcg gcggcgactc atggccgaca tgagagccgt gcccgtgcgc atgggaatcg
     1741 tcctcgtgac cgtgcccggc cccgaggtcg ccgtgaagtg catgcgagtc aacggcgctg
     1801 gcgagctcgg gcgcctcggg atcgtcgggg ctggggagct tttcgattcc ccagaaggtc
     1861 atgaagaacg cccgccccgt gtaaaatgct gtcaggaacg ccgtgaacac ggcgagccag
     1921 taaatgagaa gataaatcca cccgccggta tgcgccgcca gcttgagcga cagcaggatt
     1981 tcgtctttgc tgaaaaagcc ggagagtgga aagatgcccg acaaggccaa gccccccacc
     2041 gcgaaggtgc ggaatgtgtg tggcaaccgg tgcctgaggc cgcggaaccg ccgcatgtcg
     2101 atgacgtctc ccatcgcgtg catcacgctt ccagcagcga ggaacaagag ggccttgaaa
     2161 aacgcatggg tgaaaaggtg aaacatcgcc gccacgatag cgagctgcgc gacattgctt
     2221 acgcctgacc cgagcgccat gaacatgtag ccgagctggc tgaccgtcga gtaggcaagt
     2281 acacgcttca agtcggtctg ggtcacagcg atcgcggccg acaagagcgc cgtcgcgcag
     2341 ccgatcgacg cgatcaagag gaggaccccc ggggcgagcg tcaagagcgg catcgaccgc
     2401 gcgatcaagt aaacccccgc cgtgaccatc gtcgcggcgt ggatcaaggc agagaccggc
     2461 gtggggcctt ccattgcatc cggcagccag acgtaaagtg gaatctgcgc gctcttggca
     2521 gtcgccgccc agaataagag cagtccgatc cctacgaccg ccccaggcgg cattccccgc
     2581 agggttgact cggctagcac attaccgtac gagaggtcgt gattgggcac gatcgaccac
     2641 agccagaaga tggcgatcgc aaagccgaca tcgccgattc gattgaccag aaacgccttg
     2701 atcgcagccg ccgtggccga cggccgcgtg tgccagaatc cgatcaataa ataactgcaa
     2761 acccccacgc cctcccagaa ggcgtaggtc aagacataat tattcgagag aaccagaccg
     2821 gtcatggaga aaacgaagag cccgatcagc gcgaaaaagc ggggatatga cgggtcgccc
     2881 gccatgtagc ctgccgcgaa cacggccacg agcgatgaga caaacgtcac catcgccagc
     2941 atcatcgtgg tcaggccgtc gatccgaaat tcaatcggca cctcgaggtt gctgatcgac
     3001 agccagcgca tcacgagggt cgtttttccg ggatccgccg cgaagagcaa tcccgctgag
     3061 agcaggaacg ccagagcgat acccgcgatc accggtatgt gggcaacggc acgcacaggc
     3121 tttggcccga aagcggccag tccggcgccc atgagtggag acaatggaat caaccacgcg
     3181 atacttacga aagaatccga cacctgaccg ctcctcgatc cgctcagtct gaagtttgct
     3241 tgacggtcaa atcgctgcta aagctactcg acccacctga atgagcgagc gttccagccg
     3301 gcccagtgca gccgttgcgc ctcgtggctc acactcgcgg tcctgatttt tcaattccgc
     3361 cgctcaatcc acggctgcgt gggatggggc ggctcgatgc cggccggaac caggtgggga
     3421 tacgactcgg gaccaagcgt tggttgaacg ggttcggcgt cgagagtttc ctcggcgatg
     3481 ggcgacgagg ccggcacaac ggcctcgcgg atgtcttgcc agagagtgac gtcgagcgac
     3541 gaccgctggt tgtaaagtac cacaatcagc gccagggcaa cggaagcctc gcacgccgcg
     3601 accgtgagta tcacgatcgt gaaaatctga ccggtccagc tgccgtgaaa tcggccgaaa
     3661 ccgaccaagg aaagcgctat cccttgcagc atcatctcta ccgaaaggaa catcacgatc
     3721 aggttccgcc gcgccaggaa gcccagcatc cccaacgcga agagtgcggc acccaccaag
     3781 aggtagttcg tgagaatgtc cagtgtgaac tcatcggtcg gagccatcag cgtattcttt
     3841 cgcttgcaag aaatagggcc aagaacggcc attgccatca cgagccagca cgttccgttt
     3901 tcttggggtt ggcgatcgcg accgcaccga cgagcgcgac gaagagcaat actccggtaa
     3961 gcccggcggt taccagatga tcggtgtaga gagcctcacc caatcccgcc acgttcaatt
     4021 tctcttttcc cttggtgtcg taaatccagg atgtcggccg gtaggcgctg gccagtaccg
     4081 ccagatgttc gcccatcggc tgcagcgaga aaacttgggg gatctcgcgc gtccgcttca
     4141 atgaacctga ttggatcggt gtgggcccac cgtcatgcgg gtgcgttctc agagtcgaaa
     4201 gtgagtagat aagggaccac aacagcaaga aacaggtgaa agtagctcgg cctggcgcgc
     4261 gtgacgctcg atcgtacagt gccttcccct ccacctgggc cagcatgatc acgaagagaa
     4321 aggtgacgat gatggcaccc gcataaacga tgaccgtgcc agcggccaga aacggggcgc
     4381 ccgccaagag aaacagcccc gacgtcgcca gtacgaccga ggcgaaccaa agagcgctgt
     4441 aaacggggtt gcggctggtc acagtgagga gcgcgccgat cgtcgcggcc aggctgaaga
     4501 tgtagaaaaa gacagtggtc acgaaggggc ccggtgggct ccagaacagg aggaaagcca
     4561 gtagccccaa gctcgcactg attgcgccgg ctccatgcac ccggcgtggc cgtgcgccac
     4621 catgttggtg cggtagcagc aaatacgtgc cggccgcgcc cagcacgacg acaagcgtgg
     4681 ccagaatcgc gtcaccgatc gtcatcgtgg tcaagctttc cttcgtggta catgggttct
     4741 agtcaggcac ttcgaccgga tcttggccga tcactcgagc gacggcaaga tggtagtcgc
     4801 cgaactctgc tcggccggcg gtttgccgta gcgcatcggc tcgtggcttt tggtggtgtc
     4861 gaagaccgac aggagcttga ccttgtcgaa gatcatctcc tcgcgcgaca acccggtcag
     4921 gtcgtaaagg cccgtcagct cgatggcctc gaccggacac gcctcctcgc acatcccgca
     4981 gtaaatgcaa cgcaactcgt caatgacaaa actctccgga tacttttcgc gatccggcca
     5041 ggtggaaggc gcggtcgccc ccacgatgtc gatacagtga gccggacagg cagtcgcgca
     5101 gagcatgcag gcgacgcact tcacgcggcc ttcctcgtct ttgttgagtc ggtgcacgcc
     5161 gcggtaattc gggctcggga catgctgttc ctcggggtat tgcacggtga tcgccttgtt
     5221 ggcaaacatc acgcctgcaa tgtgtctggc tgtgaccccg agccccacgg cgatctgagg
     5281 caggaaaaac ttctcccacg ccttgagttg tggtggctcc agtcgtagaa gctttgggtc
     5341 gtcggctcgc actgccacgg ctccctttgt ttaaagagca ccaagtcgaa aatagatgaa
     5401 aaaccggtca agatcgcaac atctgcacga tcacggccgt cgcgaccagg ttcacgagtg
     5461 ccagcggtat caacgacttc caggccagat ccatgagctg atcgtaacga aagcggggca
     5521 aagtccagcg cacccacatc atgaatagga tgacgagcgc gaccttaatc aagatcacca
     5581 tcagcttggc cacggccgac agcaagccgg tttgttcctg cgacaacagc ggaaagtccc
     5641 agccgccaaa aaagaggatg actgtcagga aactcacagt gatcacatga acgtattcgc
     5701 cgagaaagaa catgccgaac ttcattcccg aatactcggt atgaaaaccc cccaccagtt
     5761 cctgctcgga ctcagggagg tcaaacggca ggcggttggt ctcggcgaac gcgcttacga
     5821 gaaagatcaa gaatccgatc ggcgaggcga tgacgcccca aacgtgacgg tcctgccagt
     5881 tgataatcgt cgagaggtcg agcgatcccg cccagaggac catccccagg atcgatagcc
     5941 ccaggggaac ctcataactg atcaactggg cactcgaacg caagccgccg atgaatgaat
     6001 acttgttgtt cgacgcccag ccggccagga tgactccata aaccgccaga ctaccgacgg
     6061 cgaaaatata aacgatgcct acgtcgacac cgggggctat ctggaaatcg attgtctgac
     6121 caagcccgac tggcccgaac ggcacaacgg caaaagcaat caaggtcgcg actattgcga
     6181 tcatgggggc tagaaggtag aggggcttgt tgacataacc cggaatgaca ccttctttca
     6241 acagcatctt ggcgccatcg gccaagggct gcagaagtcc gaaggggacg ccaaactccc
     6301 cgccggcccg gttgggtccg atccgatcct gcgcgtaggc ggcgatcttg cgctcgacat
     6361 aaatcaggta cgcgacagcg cccaagagca cgttcgtcac aattaaaatc ttcaagagca
     6421 cgccgatcag caaccacata gattcgatcc ttcaccctca ttttccgcgc cgcttgacgg
     6481 cgcggttagt atagcaagcc acaatcgtgt gatcacgcct ttagcgccag cgcgccgccc
     6541 cgtgggcgac gaaccaggcg tcgttgaagc ttggcggcac gggcgccttc gtggctggcg
     6601 gtggcgactt gccgatcacg acgccgtagg ggggtagttt acctcccttg gcgagaccga
     6661 aggccgggat cgtctccgcc agttcggcca ggatttcgct ggaacagacc ggtccaccgg
     6721 cgcgatcgag gaggattccc aggatgtcga ggtcgggcaa agaaccgtcc cgcggcggca
     6781 aggccgcctc ggaatactgg agccgcccgc tcgcattgac atagctgccc gccttctcgg
     6841 cgaaggtggc gcccgccatg accacgtcgg caagatgcgc cagcggcgtg accgtcgtat
     6901 cctggaccac cagaaaactg acctttgccc gcagcgagtt gctctggtca agatcgatcc
     6961 agggttcgat ggcgtcggaa acgacgtaca gtccgctcaa ttcgccggcg ccgacccgct
     7021 tggccagctc atcgaaggcg atgaccttcc cctcgaagtg ctcgagaacc gcggccaccc
     7081 cctgccgatt cgggcacttt tcggcgtgga tcgtgaacgg acgcggaaca acgaagctgg
     7141 tgtcgcccgt acgtcccttg gtctggtcgg gctgaaaggt ctggtcggtg ccgtgcgccg
     7201 gcaccggacc caaggcaagc acgttgcctg ggtttaatcc ttttacataa cgagcgagca
     7261 ggtacgcttc ctcgacggtc aagaacggtg aaagcacggc ggcaacgagc ccaccgcgct
     7321 ggccgatctc tttgaagccg cgatcgactg cctgaatggc ttcgccgaat gcgacaggct
     7381 ggaggtcgtt gttttttcgg acatacatcg ccgccagcag gttgggatcg ttggccgcct
     7441 tgtaactgta gcgtccctcg tcgcaaatcc agtagtcgtt gaccaaggga ttgtgccgcg
     7501 gcttgaaccg ccagagcgcg ccgcggtttt cttcggatga gatgttgcac cccgtcgaac
     7561 agcgcgtgca gatcgagtcg tgcttggaca gaaaccaggc acgctgcttg tgcagaaagt
     7621 ctttatcgag caaggctcct accgggcaca ggtcgacgat gttgccggcc aaggggttgt
     7681 cgaccggctc gcccggaaag acatcaatct cggcatgact gccccggcgc atcacctcta
     7741 actcgccggt ctgtgagatc tcgcgggtga accgcacgca ccgcgtgcac atgatgcagc
     7801 ggtccatgtt gagctggatc aggtccgaaa cgtccttgcg cgggttgacc gttcgttctt
     7861 cgacaaagcg atgaaccgat tggccgtagc gatagctgta atcctgcaag ccgcattcgc
     7921 cggcctgatc gcagacgggg caatccagcg ggtgattgag cagcagatac tccatgatca
     7981 tccgctgatg ttccttgacc ttggggctgt tggtaacgag caccgtcagg tctttggcgg
     8041 gcgtctggca accgggcaca agcttgggga gcaacttgat ctcgccggtc ttggcgtcct
     8101 tggtgccgac ttcgatctcg cacatcctgc agttggcaac caccgagagc gcagggtgcc
     8161 agcaataata aggaatgtcg atccctacgc gctctgccat ctggatcgcg ttgagctttt
     8221 caccttcggg aatcgtgtac tcgttgctat taatgatgat ggttgccatg gacgtccttt
     8281 tacccgatcg aggtgattcc agattcaact attgcaggtt tcaaaaattc attctcgatc
     8341 cgcgagggtg gcacagtcgg ttcaagccgg ttcaggccga ggctggtgga gcgccaggga
     8401 agcgcggcca ttgcccgtgt ttgacagcac ggtgagccgg ccggcggcga ggtcgaggtc
     8461 ggccgggatc ccgtgggcga tcttttcctg gagcggcttc acctgggccg cgggcgactg
     8521 atgagtccga atatagtctt cgagctcacc gcgaaacttg gccacggcgt tcttgatcgg
     8581 ccaggcggcg ccgtcggcta ggccgcagat tgtggtcccc ggaatgattc ccatgttatc
     8641 ggcgaggtta agcaggatgt cgaggtcttc ggaccggccg ccgccgaact tgatccgctt
     8701 tattgtcttg tagaaccagg tagtcccttc gcggcagggg gtgcactggc cgcagctctc
     8761 atgggcgaag aatcggcagg tgttgtacag atagtcgacg atactggtct gatcgtcgac
     8821 cacggtcacc gcggccgtgc ccaggcccag gcagcccggt ttgcgcaggc tctcgaaatc
     8881 gagtggcgta tcgagttctg cggccgacaa cagacccatg ctgattccgc ccggcacggc
     8941 cgccttggcc ttgcgccctt tccagacgcc gccggcgtgc tcgtcgatga gcgtgcggca
     9001 agtgaccccc agcgccagct cgacgcatac ctgtttgttc acatggcccg agacgcaata
     9061 aagcttcgga ccatagctct tgggggttcc gatcgatttg aaccaactcg caccccgctc
     9121 gatgatatgc gggacgcagc acagcgtttc gacattgtta acgacggtgg gcttgcgaaa
     9181 ggcgccttcg atcgccggaa acggcggttt gatccgaggc cagccgcgct tgccttccag
     9241 gctttcgatc agccc
//
LOCUS       DATAQL010000151         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2917123, whole genome shotgun sequence.
ACCESSION   DATAQL010000151 DATAQL010000000
VERSION     DATAQL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2917123"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VHS97_02550"
     CDS             complement(<1..1352)
                     /locus_tag="VHS97_02550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3447101.1"
                     /translation="MALNTAPDTDDSPAIRFSRLWETSLAIPDVFAFLRLYPDLFPID
                     RLDVLLVDQKERWRRRQPLPLRIYFSAFPDIAAEGEMVRALADGDRRERRRSAGLPSD
                     TPGMKTLDILSEAPTQPVEGESERADTEVERPGPGQVEPVPGIRCAPEDGLRTTKGPA
                     VLPPTSDRMSFALDETHHLQSEAESLRAMLNAVRFTLVCRLGAGGMGVVYETYDQERG
                     ELVALKTMRRVDPIALVRFKQEFRSLSDITHPNLVNLYELFAVDDRWFFTMELVEGCD
                     FVTYVRTQVDSLAFRADALTLPDGDPRGVVPPPAQDQESKPARSFDEMRLRLSLTQLA
                     EGVNALHQSGKLHRDIKPPNVLVNMEGRVVLLDFGLTADLESLARQQAVDRQIVGTVG
                     HMSPEQASGRSVTAASDWYSVGVMLYEAMTGQLPFAGSPDQVFIAKQTSPPQSPDAIV
                     P"
     gene            complement(1583..3187)
                     /locus_tag="VHS97_02555"
     CDS             complement(1583..3187)
                     /locus_tag="VHS97_02555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX3447102.1"
                     /translation="MNPSNALGDFQQTALILIPEFILLFTAVGIMTASAFVSLSRRFW
                     CAISAGALLAALLALLNLSDRQTGLYSAVALNDDLSFYARFVLLCTGLVLLALAHREP
                     DDERAGEFFGALLMVNAGAMLVAAANELVFLFVGLELVSIPTYVLLYLSRRSRATQEA
                     ATKYFFLSIFSSGLLLYGLTFLYGTIGISNLKAMAFLFDKLANMPQDQLGLIAVVFVM
                     AGLCFRVAAVPLHFYAPDVYEGSPIAIAATLSWVPKAVGFLAIVRALTAVLATKDPGS
                     SHLLHGAVMLAWVIAAATMIWGNFVALLQDNLKRLLAYSSIAHAGYIMVGVAAAFAGE
                     SQGGGMYYGSEGVFFYLVAYAVMTLGAFGVISALRIKGQVAQTVEDLSGLGWTHPLPA
                     LALSICLLSLAGIPPLVGFWAKFEIFTALLAASQRVESGSFIALAIIGMLSAAAGAYY
                     YLRLVVVMYLRPSKEEVRLSGGWPVALAAGACASLTLFFGLFSSPVSSAARAAALAAL
                     HHSPSQHSQVAAAVPSPQLTGRSIHN"
     gene            complement(3233..>3712)
                     /locus_tag="VHS97_02560"
     CDS             complement(3233..>3712)
                     /locus_tag="VHS97_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447103.1"
                     /translation="NRRAGVLAATGMILGACYLLWMLRKVIFGPLLEPAHQDAAGEAP
                     SHHAPGHNTCSPVGWHEIAGLTPIMGLIVAIGVFPRPFLEQIRPAVARLDDNVQSQRA
                     LANKPREVPQANPPGSISQGRRGGGAPAKKKGGQPRPKAGSAAPKGSTASANNTPKQ"
BASE COUNT          804 a         1120 c         1147 g          641 t
ORIGIN      
        1 ggtacgatcg catcaggcga ttggggggga ctggtctgtt tcgcaatgaa tacctgatcg
       61 ggtgagcccg cgaacgggag ctgtcccgtc atcgcctcgt aaagcatgac cccgacgctg
      121 taccagtcgc tggcggcggt aaccgaccgg ccggacgctt gctccggcga catgtggccg
      181 acggtcccaa cgatctggcg gtcgaccgcc tgttgccgag ccaggctctc aaggtcggcc
      241 gtcagcccaa aatcgaggag aacgacccgg ccctccatgt tcaccaggac gttgggcggc
      301 ttgatgtcac gatgcagctt gccagattgg tggagagcat tcacaccctc ggcaagctga
      361 gtcagtgata aacgaagccg catttcgtca aatgagcgag caggcttcga ctcctgatct
      421 tgcgctggcg gtggaactac tccccggggg tcgccgtctg gcagggtcag cgcatccgct
      481 ctaaatgcaa gcgagtcgac ctgggtcctc acgtaggtga caaagtcgca gccttcgacc
      541 aactccatgg taaagaacca gcggtcgtcg accgcgaaca attcgtacaa attgacaaga
      601 ttgggatgtg tgatatcgct cagggaccga aattcctgct tgaagcggac cagggcgatg
      661 gggtcgaccc gacgcatggt tttgagtgcg accagctcgc cgcgctcctg gtcgtaggtc
      721 tcatatacta cccccattcc gccggcccct aaccggcaca cgagcgtgaa gcgcaccgcg
      781 ttgagcatcg ctcgcaaact ttcggcttcc gactggaggt gatgagtctc atcgagggcg
      841 aacgacattc gatcggatgt cggtgggaga accgccggcc ccttggtggt gcgcaatcca
      901 tcttcgggag cacatcggat tcccggcacg ggctcgacct gcccgggccc aggccgctcg
      961 acttcggtgt ctgcacgctc ggattcgccc tcgacaggct gcgtcggtgc ttccgacaaa
     1021 atgtcgagcg ttttcatccc gggcgtgtcg ctcggcaagc cagccgaccg ccgtctctcg
     1081 cgacggtcgc catcggccag cgcgcgaacc atttcccctt cggcggcaat atccgggaag
     1141 gccgaaaagt agattctgag gggcaacggc tggcggcgac gccagcgctc cttctgatca
     1201 acgagcagca catcgagccg atcgatcggg aaaagatccg gatataagcg aagaaaagcg
     1261 aacacgtcag gaatcgccag gctcgtctcc caaagcctgg aaaaacggat cgccgggcta
     1321 tcgtcggtgt ctggagcggt gttcaatgcc atcgccgtgt cgaccgccgt cattcgtttg
     1381 gatgcatgct cgaagtgacg acaggataaa ctcgcccgta acacgaaaat gatccgcgcc
     1441 gcaccgccct ggtcggtgct ctccgcggtc gccaccgaga gtgtagccag aaactacgcc
     1501 ggctgccagc gagcccaagc tccctgcttt ccctcgaccg ttgaccgtac cagttaaaga
     1561 tacggacaaa agcgccgcgc gatcaattgt gaattgagcg acccgtgagt tggggtgatg
     1621 ggactgcggc ggctacctgc gaatgctgag acggagaatg atggagtgcc gccagcgcgg
     1681 ccgctcgggc cgcgctcgat acgggcgacg agaacaggcc gaagaagaga gtcaagctcg
     1741 cgcacgcacc ggcggccaag gccacgggcc agccgccgct gagccgcact tcttccttgg
     1801 acggacgcaa gtacatgacg acaacaagcc ggagataata gtacgcgccc gccgcagcgc
     1861 tcagcatacc gatgatcgcc agcgcgatga acgaacccga ttccacacgc tggctcgcgg
     1921 ccaagagtgc ggtaaagatc tcgaattttg cccagaagcc caccaggggt gggatgccgg
     1981 ccaaactaag caaacagatc gacagagcca gggccggcaa cggatgggtc cagcccaaac
     2041 cggataaatc ttcaactgtt tgtgcaacct ggcctttgat gcgtagggcc gagatcactc
     2101 cgaaggcgcc cagcgtcatt acggcatacg cgaccaggta gaaaaagacg ccttcggaac
     2161 cataatacat accgccgccc tgggattcgc cggcgaacgc ggctgccacg ccgaccataa
     2221 tgtaccccgc gtgagcaatc gacgaatacg ccagcagccg tttgaggttg tcctggagca
     2281 aggcgacgaa gttgccccag atcatggtcg cggcggcgat aacccacgcg agcatgaccg
     2341 caccgtggag caaatgggaa ctgcctgggt ccttggtcgc caggacagcc gtgagggccc
     2401 gcacgatcgc caggaatccg accgccttgg gcacccatga cagcgtcgcg gcgatcgcaa
     2461 tgggcgagcc ctcgtagacg tctggggcgt aaaaatggag aggcaccgcc gccactcgga
     2521 agcaaagtcc tgccatcacg aacacaaccg cgatcaagcc gagctggtcc tgcggcatat
     2581 tcgccagctt gtcgaacagg aacgccattg ctttcaagtt gctgattccg atcgttccgt
     2641 acaggaatgt caggccatag aggagcaggc cggatgaaaa aatgctgagg aagaagtatt
     2701 tggtagccgc ttcttgcgtg gcccgactgc ggcgcgacag gtaaagcagc acgtaggtcg
     2761 gtatactcac gagctcgagc ccgacgaata agaacaccag ttcgttggcc gcggccacca
     2821 gcatagcccc ggcattgacc atgagtaagg caccgaaaaa ctcgccggcg cgctcgtcgt
     2881 cgggctctcg gtgcgcaagc gccagcaaga cgaggccggt acacaaaagc acaaaccgcg
     2941 cgtaaaacga caaatcgtcg ttcaatgcga cggccgaata gagtccggtc tggcggtcgc
     3001 tcaagttcaa gagggccagt agcgccgcca agagtgcgcc tgcgctgatc gcgcaccaga
     3061 atcggcgtga caggctgaca aacgccgacg ccgtcatgat ccctacggcc gtgaacaaca
     3121 ggatgaattc ggggatcaaa atcagtgcgg tctgctggaa gtcgccgaga gcattcgagg
     3181 gattcatagc cgcttttctc gtgaatttgc gtcgggcaaa gatgagccga ggttattgct
     3241 tgggggtgtt gttcgctgag gctgtagaac ccttgggcgc agccgaacct gccttgggcc
     3301 tgggctgccc gcctttcttc ttggccggag caccaccccc tcgtctccct tggcttatac
     3361 tccctggagg attcgcctgg gggacctcgc gaggtttgtt cgctaaagcg cgctgggact
     3421 gaacgttgtc gtccaagcgg gcgaccgcag ggcgaatctg ttccaggaac ggccggggaa
     3481 agacaccgat cgccacgatc aagcccatga tgggagtcag gccggcgatt tcatgccagc
     3541 cgacgggaga acacgtgtta tggcccggag cgtgatgcga tggagcctcg ccagcggcgt
     3601 cttggtgtgc aggctcgagc agtgggccga agatgacttt gcgtagcatc caaagcaggt
     3661 agcacgcgcc caggatcatg ccggtcgctg ccaggacgcc ggcccgccgg tt
//
LOCUS       DATAQL010000152         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_17066, whole genome shotgun sequence.
ACCESSION   DATAQL010000152 DATAQL010000000
VERSION     DATAQL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17066"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VHS97_02565"
     CDS             <1..1265
                     /locus_tag="VHS97_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX3447104.1"
                     /translation="IWLVVRGRAPDRVLWIVTGVGVVASVIRLVFVYGHGALGSDYRI
                     FYEAGRDILAGISPYSPSRFASHPFLNPPSMFPFFAVLALPPYPTSLAVWTLVNSVMA
                     FALVWQARRTLLVQEGGTTSRLSHAELGALAAAFGLSDACTTTINLGQLSLLATVLIL
                     LALEFQGRQMPARAGGVLGVAAMKVGTMLPFLLLFCRKQDRRSWIVLVATTAALILLG
                     GRAPQIFEDVRSMLHYIGEFSKPGQTNDISYQGPLHWTILGFDHLAYRLGMRERTMLA
                     VFQFVGLGLIGGWLAWEVIAGRVTRAQGIVLLSLYSVVFLYHRMYDAVMFVPALVYSV
                     EQAKNRRGRCHFLFTGAMLSMLFVLYMRQKTLAKLTDWVTTHHGVAASLVETLILPQA
                     VWLILLSMVLLRVGMADAKRPDSLGYIT"
     gene            complement(1467..2642)
                     /locus_tag="VHS97_02570"
     CDS             complement(1467..2642)
                     /locus_tag="VHS97_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447105.1"
                     /translation="MPTISAGSKASLTPSPAGVPEPTVPGSSGNWIWPSWRNLILFAL
                     LSRVVVFAVGFMVVGGGSDNSRRWEAFRRFDAIWYEQIAVGGYDEYRPARENRIAFLP
                     ALPIAMASGGVLGVSPKVMGVLAPNLAFVVGLACFGRAALRVTGQSGTAWRACLLLTA
                     YPYSFFFSAPYQESLGLALMAAGLVLWVEDRPWAAILVLPWAPLARLQGVSLPAAIGI
                     DWVVARLRGRASRRWACLVALSGGLGLLLFGLYCYQKYGDPLIHLKAHGMWGRKPASL
                     PNFARSLVAPAVRWWEGSRLDLKNYAIAVLFLGLAAHAWWRRGLFWAILIAVPILEGM
                     VNDTLMGMGRIALMAFAGFIDLAEILNLRITFWGGMAVMVLVQVYLIWQYSHWIFVA"
     gene            complement(2705..>3329)
                     /locus_tag="VHS97_02575"
     CDS             complement(2705..>3329)
                     /locus_tag="VHS97_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447106.1"
                     /translation="RRRVNWIARQIKITSNYVQITSSTSVLTPSPPCGGSQGGVFLAW
                     FNFGIFAGDKSWVAGVELATASEPPARKPRIWGRRPSGVDPSHPLRWNLLLNLSISDA
                     AAPQWAMTQPVSVASVFRLMYSSTSIENEAPAKSRRFRPLALRLVVGVYKGGHDHDCV
                     VKAVIVVRPLMRNAVDSTGLQAQTVALFGRSWVSLARTRAPRLSVDC"
BASE COUNT          682 a         1064 c          959 g          624 t
ORIGIN      
        1 ttatctggct tgtcgtgcgt ggacgagcgc cggatcgcgt gctctggatc gttaccggag
       61 tcggcgtagt cgcttcggtg atccgtctgg tcttcgtcta cgggcacggg gccctggggt
      121 ctgactatcg gatcttctat gaggcaggtc gcgacatcct ggccggaatc tccccttact
      181 caccgagccg gttcgcaagt cacccgttcc tgaacccgcc gtcgatgttc ccgttcttcg
      241 ctgtgctagc tctgccgccg tacccgacga gtcttgccgt ctggacgctc gtcaattcag
      301 tcatggcgtt cgccctggtc tggcaggcga ggcgaaccct ccttgtgcaa gagggtggaa
      361 cgacttcccg gttatcccac gccgagctcg gcgcactggc cgccgccttc ggcctgtccg
      421 atgcgtgcac gacgacgatc aatctgggcc agctttcgct cttggccacg gtcctcatcc
      481 tcctggcgct cgagttccag gggcgtcaga tgccggcgcg ggcgggtggg gtcctcggag
      541 tcgccgccat gaaggtgggc acaatgctcc ccttcttact gctcttctgt cgaaagcaag
      601 atcgcaggag ctggatcgtt ctcgtggcca cgaccgcggc cttgatcctc ctgggagggc
      661 gggcgccgca aatattcgaa gacgttcggt cgatgctcca ctacatcggc gagttctcga
      721 agcccgggca gaccaacgac atttcttatc aagggcctct ccactggacg attttgggct
      781 tcgaccatct ggcctaccgc ctggggatgc gggagcggac gatgctggcc gttttccagt
      841 tcgtcggcct cggcttgatc ggaggttggc tcgcgtggga ggtcatcgcc ggtcgggtca
      901 ctcgggcaca ggggatcgta ctcctgtcgc tctactccgt cgttttcctc tatcaccgca
      961 tgtacgacgc agtcatgttc gtgcccgcct tggtgtactc cgtcgaacag gccaagaacc
     1021 ggcgaggccg atgccacttt ctcttcaccg gggccatgtt gtcgatgctt ttcgtacttt
     1081 acatgaggca gaaaactctg gcaaagctca ccgattgggt aacgacccac catggcgtgg
     1141 ccgcgtcgct ggtcgaaacg ctaattcttc cccaggcagt gtggctgatc ctgctgtcga
     1201 tggtcttgct gagggtcgga atggccgacg ccaaaagacc ggactcgttg ggctacataa
     1261 cataacttga cggcgggttg cccggaccag tgtgcaacac gggattcccg actttgggta
     1321 cgagcgctgc cagttcggat acgtcgagta cgttgggccc gttgaaattc cacgaaaaac
     1381 tactgcggcc agaccaccag cgacgtgctc cgcgacttgc tcaacctggg cgtcaccttt
     1441 cgagccacac gtgccctagc cgacgatcag gcgacgaaaa tccagtgaga gtattgccag
     1501 atcagatata cctgcacgag gaccatcacg gccatccccc cccagaaggt gatccggaga
     1561 ttcaggatct cggccaggtc gatgaagccg gcaaacgcca tcaatgcgat ccggcccatc
     1621 cccattaatg tgtcgttcac cattccttca aggatcggca ccgcgatcag aatggcccaa
     1681 aacaaacccc gccgccacca agcatgcgca gccagaccga ggaaaaggac tgcgatcgcg
     1741 tagtttttca ggtcgagcct cgacccttcc caccagcgga ccgctggtgc cacaagactc
     1801 cgggcgaagt tcggcaggct ggcgggctta cgcccccaca tcccatgggc tttcaggtgg
     1861 atcagcggat caccgtattt ttgatagcag tagaggccaa agagcagcag gcccagccca
     1921 ccggacagcg caaccagaca ggcccaacgt cgggaggctc ggccccgaag ccgggcgacg
     1981 acccaatcga tcccaatcgc cgccggcagc gagaccccct gtaagcgagc caacggagcc
     2041 cagggcaaga ccaggatggc cgcccaggga cgatcctcca cccacaacac gagcccggcc
     2101 gccatcagtg ccagtccgag cgattcttga taaggcgccg agaagaagaa cgaatagggg
     2161 taagccgtca gcagcaaaca ggctcgccaa gccgttcccg attggcccgt aacgcggagc
     2221 gcggcccgcc cgaagcaggc caagcccacg acgaaagcca ggttcggcgc tagcacaccc
     2281 attactttgg gcgacacccc caacacccca cctgaggcca tcgcgatcgg cagcgccggc
     2341 aagaaggcga tacggttctc gcgcgcaggg cgatactcgt cgtagccacc tacggcgatc
     2401 tgttcatacc atatagcgtc aaacctccga aaagcttccc accgacgcga gttgtcggac
     2461 cctcctccaa cgaccataaa accaaccgcg aagacgacga ccctcgacaa caatgcgaag
     2521 aggatcaagt ttctccacga gggccagatc cagttgccag agctacctgg gaccgtgggt
     2581 tcggggacgc cggctggcga aggcgtgagc gaagctttgg aacccgccga aatggtcggc
     2641 actaaagaag ttcctgttaa ggagtacaaa aataaggcgc ggtcgtccac gcattcgagt
     2701 tcgatcaaca atcaacactt aagcgcggtg ctcttgtcct tgctaaagat acccaagagc
     2761 gtccaaagag agctactgtc tgagcctgta acccggtcga gtcaacggca tttcgcatca
     2821 agggccttac aactatcacc gcctttacga cgcagtcatg gtcgtgcccg cccttgtata
     2881 cgccgacgac cagccgaaga gccagaggcc ggaaccgcct gctcttcgcc ggcgcctcgt
     2941 tctcaatgct tgtgctgctg tacatgaggc ggaaaacgct cgcaacgctc acaggttggg
     3001 tcatggccca ctgcggcgcg gccgcgtcgc tgatcgaaag gttcaaaagc aggttccacc
     3061 tcagcgggtg gctggggtcg acgcccgaag gtcgacgccc ccagatccga ggcttccgcg
     3121 ccgggggctc gctggctgtc gccagctcga ccccagccac ccagctcttg tcacctgcga
     3181 agatgccaaa gttgaaccac gccagaaaaa cccctccctg tgagcccccg caaggggggg
     3241 agggtgtcaa aaccgacgtt gagctagtaa tttgcacata attcgacgtt attttgattt
     3301 gacgggcgat ccaattgacc cgccgacgg
//
LOCUS       DATAQL010000153         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2612231, whole genome shotgun sequence.
ACCESSION   DATAQL010000153 DATAQL010000000
VERSION     DATAQL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612231"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VHS97_02580"
     CDS             complement(<1..1004)
                     /locus_tag="VHS97_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX3447107.1"
                     /translation="MAEKLIPATVIGSWSFPGWFEKFVADVKGHPERFGPADRVEAVR
                     DAVRLAIDDQVRAGLDRITDGEMQRVDFNLGFYEYLHGLESAPRVRRWGAPAHDQRDR
                     YRAVEPITAPDGLGTVAEYRRLREYTDAPVKVPVPGPFTLAGCIDGGAVYADRTAVTD
                     ALIPIVNGELRALAASGVDFIQLDEPSFACHPKEPESFLEVIARTVEGANAYISMHMC
                     FGNYRARAVGHRSYRPLFPHLGRARVNQLALEFASRELAELDLLSQLPESMDVAIGLI
                     DVKNTWVEPPELVAERLRTVLKYIDAARVSVTPDCGFSQTAGISRLPRPGRWLRGSSW
                     "
     gene            1189..>2140
                     /locus_tag="VHS97_02585"
     CDS             1189..>2140
                     /locus_tag="VHS97_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447108.1"
                     /translation="MKAWSWVIFAAVGWGVMLAAYNTRAQQDRGAAWAYGFFVIGPPL
                     PPEIAQTERAIPVRLRLVPAVERTESILKVMSQLAKDKVANLESQLRPKLILLPLAEA
                     GIDASSLQSGRLPEAGRDQVIAGPSAAHKDRLTVGDRVLDVVGVLKGEFALLRNDYLI
                     PPSEPANALFADGEPSVHPATLVRLTYEQSHDRHFLGELLKPLSPPEYSVVMPQERLD
                     PVAFYSYAGGMAAFLLGGSGALIGLFRWLAARSRGPLLSNGDEHFGEVAEPETTKPQT
                     SWWAKPLLEMERRPRLVWGVHLVYFGLVIAFSVLVYQLPDV"
BASE COUNT          408 a          644 c          658 g          430 t
ORIGIN      
        1 aaccagcttg accccctcaa ccatcgccct ggccttggca atcgcgatat gcctgccgtc
       61 tgcgaaaagc cgcaatcggg agtcaccgaa acgcgtgcgg catcgatgta tttcagcacg
      121 gtccgcaatc gttcggccac cagctcgggt ggttcgaccc aggtgttctt gacgtcgatc
      181 aagccgatag ccacatccat cgattcgggc aactgagaga gcaggtcgag ttcagcgagc
      241 tcgcggctgg cgaattcgag agccagttga ttgacccgcg cgcggccgag atgcgggaac
      301 aagggccgat acgagcggtg gcccacggca cgggcgcggt aattgccaaa gcacatgtgc
      361 atgcttatgt acgcattggc tccttcgacc gtgcgggcga tcacctccag gaacgactcg
      421 ggttccttgg gatgacaggc aaagctgggc tcgtcgagct ggatgaagtc aactccggaa
      481 gccgccagag cccggagctc gccgttgaca atcgggatca atgcatcggt cacggcagtt
      541 cgatcggcat agaccgcgcc gccatcgatg cagccggcca gggtgaaggg acctggaacc
      601 ggcaccttta ccggagcgtc ggtgtattct cggagccgac gatattcggc aactgtcccc
      661 agcccgtccg gcgctgtgat gggctcgacg gcccgatagc gatcacgctg gtcgtgcgcg
      721 ggcgcgcccc agcggcggac gcgcggcgcg gactcaagtc cgtggagata ctcgtagaaa
      781 cccaggttga aatcgacccg ctgcatttcg ccgtcggtga tccggtcgag gcctgcgcgg
      841 acctggtcgt cgatcgccaa ccgaacggcg tcacggacgg cctcaacgcg atcggccggc
      901 ccgaatcgct cgggatggcc tttcacatcg gcgacgaact tttcaaacca acccgggaac
      961 gaccagctcc cgatcaccgt ggcgggtatc aacttctcag ccatcgtaat cttgctcctc
     1021 gtgcgagaat tgtcgcggct attgtcccgt ccgttatgtt tgcaagtcaa acgaagaccg
     1081 accgaacatg agaccataat tgacggcaga ctctgactag tgtaagaatg agcgcgcaac
     1141 gcgatctctc tgacattcga cactgcctcg cccgaatcga ggatgattat gaaagcttgg
     1201 tcttgggtca ttttcgctgc ggtgggctgg ggcgtgatgc ttgcggcgta caacacgcgt
     1261 gcccagcagg atcgtggggc cgcgtgggcg tatggctttt tcgtgatcgg tccgccatta
     1321 ccgccagaga tcgcgcagac tgagcgtgcc atccctgtgc ggctgcggct cgtgccggcg
     1381 gttgaacgca ccgaatccat tctcaaggtc atgagccagc tggcgaagga caaagtggcc
     1441 aatctggaat cgcagcttcg acccaagctc attctgctac cattggcgga agccggcatc
     1501 gacgcgagct cattgcaatc ggggcgattg ccggaagccg gtcgggatca ggtcatcgcg
     1561 ggtccatctg ccgcacacaa agaccggttg actgtcggtg atcgggtcct cgacgtagtc
     1621 ggagtgctca agggcgagtt cgcgctcttg cgaaacgact atctgatccc tccgtccgag
     1681 ccagcgaatg ccctgttcgc agatggcgaa ccatcggtcc atccggcgac gctcgtgcgg
     1741 ctcacttacg agcagtctca cgacaggcac ttccttgggg aattgctcaa gcccctttcg
     1801 ccgcccgagt acagcgtcgt gatgccgcag gaacgactcg atccggtagc cttctattct
     1861 tatgcgggag gcatggcggc cttccttctc ggtggctcgg gtgccttgat cggtctcttc
     1921 cgctggttgg ccgcaaggtc ccgtggacct ttactgtcga atggtgatga gcatttcggc
     1981 gaggtcgccg agccggagac gaccaaaccg caaacaagtt ggtgggcaaa accgctcttg
     2041 gagatggaac gacggccccg cttggtctgg ggtgtgcatc tggtctactt cgggctcgtg
     2101 atcgcctttt cggttttggt ttatcaactt cccgacgtcc
//
LOCUS       DATAQL010000154         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2917215, whole genome shotgun sequence.
ACCESSION   DATAQL010000154 DATAQL010000000
VERSION     DATAQL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2917215"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VHS97_02590"
     CDS             complement(<1..742)
                     /locus_tag="VHS97_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3447109.1"
                     /translation="MSVRSEPLLAAARPARRGAPIGLWLDRQSVFSWLMMAPPLLFLA
                     ALVGYPFCYGILLSLQDRPVAHPGTFVGLKNFATNIADPIFWRVAVNTFVYTGVATLL
                     KMVGGLGLAVAMNQHFRMKNLVRALLLLPFIVPTVLSTVAWMWMLDPAFSVVNRLLIG
                     LGVPRPGPSWLGNPILAMFSIIMINTWRGLPFYGITLLAGLQTVPPELYEAATIDGAS
                     GWQRFRYVTLPLLQPIILIVTLFSVIFTF"
     gene            complement(746..2044)
                     /locus_tag="VHS97_02595"
     CDS             complement(746..2044)
                     /locus_tag="VHS97_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3447110.1"
                     /translation="MTEPATSHPTTRRGFLKLAAAGTAAALSRPRPAGATERTITILH
                     ESSFIKPFDEYVVNTIAPAYEKETGIKVNYEVTSVGSLPTRISTIAETGSGADITMNG
                     LLQVIQFGDKYLDVGDLAEDIGKAQGGWYDAGREAVLVEGKWKAIPFSNIGQLMNWRT
                     DWFAEVGVKKFPETWEELYEVGKKLKAEDHPFGFELGHGFGDNHGWLYPLLWSYGGAE
                     VEPDGKTVVIDSDETARAVDFARKFFRDTMFEDVLGWTDPSNNKAWMAEQISCTNNAE
                     SILWFAKAHFPDIGKVTDQAQNPQGPKGRFHLLDCQSHAIFDFSPRKEEAHKFLRWLT
                     AEQQLGGWFASADSYYQPFLHAYDNAPMWQVEPRNIPYRDAMATAHLPGWPAPPDHRL
                     AQSVAKYVIVDMFAKACTGTATKDAIKSAEAQLKEIYRAV"
BASE COUNT          447 a          743 c          753 g          446 t
ORIGIN      
        1 cgaaggtgaa gatcaccgaa aacaacgtca caatcaggat gatcggttgc aacagcggca
       61 aggtgacgta gcggaaacgc tgccaaccgc tggcgccgtc gatcgtcgcc gcctcgtata
      121 gctcgggcgg cacggtctgc aagccggcga gcaaggtgat cccgtaaaag ggcaacccgc
      181 gccaagtgtt gatcatgatg atcgaaaaca tcgccaagat cgggttgccg agccaggagg
      241 gacccggcct cggcacgccg agcccgatca gcagccggtt aacgacactg aacgccgggt
      301 cgagcatcca catccaggca accgtgctca acacggtcgg cacgatgaac ggcagcagca
      361 acagcgcccg caccaggttc ttcatgcgga agtgctggtt catcgcgacc gcgagcccga
      421 gcccgcccac catcttcagc agcgtcgcga cgcccgtgta aacgaaggtg ttgaccgcga
      481 cgcgccagaa gatcgggtcg gcgatgttgg tcgcgaaatt cttcagtccg acaaaggtcc
      541 caggatgggc gaccggccga tcctgcaggc tcaacaaaat gccatagcag aacggatagc
      601 cgacgagagc cgccagaaac agcagcggcg gcgccatcat cagccagctg aaaacactct
      661 gacggtcgag ccataggcca atcggggcac cgcggcgcgc cggccgcgcc gccgcgagga
      721 gaggctcgga ccggaccgac attcctcaga cggcgcggta gatctctttg agctgcgcct
      781 cggcgctctt gatcgcgtcc ttggtcgcag taccggtgca cgccttggcg aacatgtcga
      841 cgatgacgta tttggcgacg ctctgggcca gccggtgatc tggcggcgcc ggccagcccg
      901 ggaggtgcgc cgtcgccatc gcgtcgcgat aggggatgtt gcgcggctcg acctgccaca
      961 tcggcgcgtt gtcgtaggca tgcaggaagg gttggtaata gctgtcggcc gaggcgaacc
     1021 agccgccgag ctgctgttcc gccgtcagcc agcgcaggaa cttgtgcgcc tcctccttcc
     1081 gggggctgaa gtcgaaaatc gcgtggctct ggcagtcgag caggtggaag cggcccttgg
     1141 gtccctgtgg gttttgcgcc tggtcggtca ccttgccaat gtccgggaag tgcgccttgg
     1201 cgaaccacag gatgctctcg gcgttgttgg tgcaggagat ctgctcggcc atccacgcct
     1261 tgttgttgct cggatcggtc cagcccagca cgtcctcgaa catcgtgtcg cggaagaatt
     1321 tgcgggcgaa atcgacggcg cgcgcggtct cgtcggagtc gatcaccacg gtcttgccgt
     1381 cgggctcaac ctcggcgccg ccataggacc acaagagcgg gtagagccag ccgtggttgt
     1441 cgccgaagcc gtggccgagc tcgaagccga agggatggtc ctcggctttc agcttcttgc
     1501 cgacctcgta gagctcttcc caggtctccg ggaacttctt gaccccgacc tcggcgaacc
     1561 agtcggtgcg ccagttcatc aattgaccga tattgctgaa ggggattgcc ttccatttgc
     1621 cctccaccag aacggcctcc cgaccggcgt cgtaccagcc gccctgcgcc ttgccgatgt
     1681 cctcagcaag gtcgccaacg tcgagatatt tgtcgccgaa ctggatcacc tggagcaggc
     1741 cgttcattgt gatgtcggcg cccgacccgg tctcggcaat cgtgctgatc cgtgtcggca
     1801 agctgccgac gctggtcacc tcgtagttga ccttgatgcc ggtctctttc tcgtaggccg
     1861 gggcaatcgt gttgaccaca tattcgtcga acggcttgat gaacgagctc tcgtgcagga
     1921 tcgtgatggt cctctccgta gcgcccgccg gacggggccg gctcagcgcc gccgcggtgc
     1981 ccgcggccgc gagcttgaga aatccccgcc gtgttgtcgg gtgacttgtt gccggctctg
     2041 tcataccgcg tcctccctga gttgatgccc tgggggattg actgcccgct tctttgcatg
     2101 ttggagccca acactacatc aatatcgggc ttggcacagt cgagaatcgg gatcgagcag
     2161 cgcgtcctta ccgttcttta gttcgagtat cggacggttt tgaaccggcc gagtaagctt
     2221 gatgaagctc cacgcaccgc gcccgcaaga aggacaacat tgctgacgaa agtgcgattg
     2281 tgcacgtgtt gaaccagagg tcgcctgatg gggtcagacc cggaactggc cgagctgata
     2341 tagaaacggc ccgttgatct tcgcgatata gatgcggccg tcctgcacc
//
LOCUS       DATAQL010000155         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_17238, whole genome shotgun sequence.
ACCESSION   DATAQL010000155 DATAQL010000000
VERSION     DATAQL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17238"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VHS97_02600"
     CDS             <1..1629
                     /locus_tag="VHS97_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3447111.1"
                     /translation="RPDVPELKAGSRYLVEVVVRTLGIGHPFSQGTVDSNEIWVELTA
                     RSGERVIGHSGGMGSDDVVDPYSHFINVYMLDRDGNRIDRRNPQDIFVPLYNKQIPPG
                     AGQVVHFGLDLPTSETGPITLEAKVNYRKFDRTYLDYIFGKGQGPKLPVVVMARDQVS
                     LPVHGGPPVTNPPSPIEATWQRYNDYGIGLFLEGASKGGQKGELKQAEHIFLKVAELG
                     RADGWVNLARVFQREGRIPDALSALEKAANHKEPAAPWVINWLTGQINASNGLLDDAI
                     TSFESVLSTKIPARKFDFSLDYRVINDLGSALYARARSEFPVVSATRRDFLHRAIAAY
                     RRTLAIDSEDVGAHWGLAQAYYQAWVDQANGATQTEPESGVKDEPVEPETLVKQATTI
                     ADPKVAALKRRELALQLARGITRFMNGERPRYQSRLEPLHDIVEILGRAWEAETDPQN
                     QAALARALEVAHRRLHERLKPDETAEGRAFALARQKDPAANHNSESIVIHSLHRPGAP
                     GSSQAVATAAIARTNENPTNTAATARPPAPEYRK"
     gene            1626..>2956
                     /locus_tag="VHS97_02605"
     CDS             1626..>2956
                     /locus_tag="VHS97_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3447112.1"
                     /translation="MSLVRIADRVKYLGWLLLFFLPAGCSPGTAKPVLPAAVAPVRPA
                     AVKAETLPAVKFIDITKEAGISFVHDNGAQGDKLLPETMGAGVAFLDYDNDGDQDLFF
                     VNSSFWPGHEIKPAPTQALYRNDGKGHFENATKEAGLDKSFYGQGVAVGDYDNDGDAD
                     LYVTAVGRGYLFRNDGKGHFDDVTLAATAKGPNGWLTGAAFIDIDNDGDLDLFIGNYV
                     TWTPEIDKVQGFQLTGIGRAYGRPTSFGGSFCALLLNEGGRFTDISEKAGIQVRTPDL
                     KVPVGKSLGVAPYDIDGDGLVDIAVANDTVQNFLFHNQGQGRFEEIAIPSGVAFDQSG
                     SPRGGMGIDWAPFMNDDRLGLAVGNFANEMTALYVCDQPKTLVFSDLANLYGLGAPTQ
                     PPLKFGLFFFDYDLDGRLDLLSANGHLEPDISKVQASETYEQPVQLFWNSG"
BASE COUNT          614 a          939 c          863 g          540 t
ORIGIN      
        1 cgtcccgacg tacccgagct caaggcaggg agccgttact tagtcgaggt cgtcgtcaga
       61 accctcggca tcggccaccc tttcagccag ggaaccgtcg attccaacga gatctgggtc
      121 gagctgaccg cacgttcggg agaacgtgtc atcggccact cgggcggtat gggatcggat
      181 gatgtcgtcg atccctactc gcacttcatc aacgtttaca tgcttgaccg cgacggcaac
      241 cggatcgatc gccgcaatcc gcaagacatc ttcgtgcccc tctacaacaa gcagattccc
      301 ccaggcgcgg gccaggtggt tcactttggg ttggacctgc ccacgagcga gaccggcccc
      361 atcaccctgg aggccaaagt caattaccgc aagttcgacc ggacctatct cgactacatc
      421 tttggcaagg ggcaggggcc caagctgcca gtggtcgtca tggcgcgcga ccaggtttca
      481 ctgccggtcc acggtggtcc ccccgtcacg aatccgcctt cgccaatcga ggcaacctgg
      541 cagcgctata acgattacgg catcgggctc ttcttggaag gtgcgagcaa ggggggacaa
      601 aagggagagc tgaaacaggc cgagcacatc ttcctcaaag tggccgaact ggggcgggcc
      661 gacggctggg taaacctggc gcgcgtcttt cagcgcgaag gccgaatacc cgacgccctg
      721 tcggctctcg aaaaggccgc caatcacaag gagcccgctg ccccctgggt tatcaactgg
      781 ctcaccgggc agatcaatgc gagcaacggc ttgctcgacg atgcgatcac cagcttcgag
      841 tcagtcttgt ccaccaaaat ccccgcgcgt aagtttgact ttagcctcga ttatcgcgtg
      901 atcaacgatc tgggctcggc tctttacgcc cgagcacgga gcgagttccc ggtcgtgagc
      961 gcaaccagac gagacttcct gcaccgggcg atcgccgcct acaggcggac gctcgccatc
     1021 gattccgagg acgtgggggc ccattgggga cttgcgcaag cctattatca agcctgggtc
     1081 gaccaggcaa atggtgccac acaaacggaa cctgaatccg gcgtcaagga cgaaccggtt
     1141 gaacccgaaa cactcgtgaa gcaggcaacc acaatcgccg atcccaaggt tgccgctcta
     1201 aagcgccggg agctggcgct acaattggca cgagggatca cgcggtttat gaatggtgaa
     1261 agaccgcgat accagtcgcg gttggagccg cttcatgaca ttgtcgagat cctgggacgt
     1321 gcgtgggagg ccgagaccga ccctcaaaat caggccgcgc tggcgcgcgc cctggaagtc
     1381 gcgcaccgga ggctgcacga aaggctcaag cctgacgaga ccgccgaagg ccgggccttc
     1441 gccctggcgc gacagaaaga cccggccgcc aatcacaact ccgagtcgat cgtaatccat
     1501 tctctgcacc gaccgggtgc gcctggaagc tctcaggcgg tggcgactgc cgctatcgca
     1561 cgaaccaacg aaaacccgac caacaccgcc gccacggcca gaccgcccgc tccggagtat
     1621 cgaaaatgag cctcgttcgc atcgccgatc gcgtgaaata cctgggttgg ctgctcttat
     1681 tcttcttgcc cgcgggttgc agccccggga ccgccaagcc ggtactgccc gcggccgtcg
     1741 cccccgtgcg gccggccgcc gtcaaggccg agacgctccc cgccgtcaag ttcatcgata
     1801 ttaccaagga agcgggcatc agtttcgttc acgacaatgg cgcccagggt gacaagctcc
     1861 tccccgaaac gatgggcgcc ggcgtggctt ttctcgacta cgacaatgac ggcgatcagg
     1921 acctgttttt cgtcaactcg tcgttctggc ccgggcacga aatcaagccg gcacctaccc
     1981 aggcccttta tcgcaatgat ggcaagggcc attttgaaaa cgccaccaaa gaggcagggc
     2041 tcgacaagtc gttctatggc caaggggtgg ctgtgggtga ttatgacaac gacggcgacg
     2101 cggacctcta tgtcacggcc gtcggccgcg gttatctctt ccgcaacgac ggcaaggggc
     2161 acttcgacga tgtcacccta gccgccactg ccaaaggtcc caacggctgg ctcaccggtg
     2221 ccgcctttat cgacatcgac aatgacggag atcttgacct ttttatcgga aattacgtta
     2281 cttggacccc cgagatcgac aaggtgcaag gatttcagct aacgggcatt ggccgggcct
     2341 acggtcggcc aacctcgttt ggcggctcgt tctgcgcact actcttgaac gaagggggac
     2401 ggtttacgga catcagcgaa aaggccggaa tccaggtgcg cacacctgac ctcaaggtgc
     2461 ccgtgggtaa gtcgctgggt gtggcaccgt acgacatcga cggcgacggg ctggttgaca
     2521 tcgccgtcgc caacgacacg gttcaaaact tcttgtttca caaccagggc cagggcaggt
     2581 tcgaggagat cgccatcccg tcgggcgtcg cgtttgatca gtccggctcg ccgcgaggag
     2641 gcatgggcat cgactgggcg ccgttcatga atgacgaccg gctaggcctg gcggtcggca
     2701 acttcgccaa cgagatgacc gcactttacg tctgcgatca gcccaagacg ctcgtgtttt
     2761 cggacctcgc caatctctac gggctcggtg ccccaacaca gccgccgctg aaattcggcc
     2821 ttttcttttt tgactacgat ctggatggcc gcctcgacct cttatccgcc aacggtcact
     2881 tggagcctga catatccaag gtgcaggcca gcgagaccta tgagcaaccc gtccagctct
     2941 tctggaattc cggtag
//
LOCUS       DATAQL010000156         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3223908, whole genome shotgun sequence.
ACCESSION   DATAQL010000156 DATAQL010000000
VERSION     DATAQL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223908"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VHS97_02610"
     CDS             <1..940
                     /locus_tag="VHS97_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3447113.1"
                     /translation="IGRIPIDTTGPDGKPGARIPLQQVVNIEAHKPGAAYIYRENNRR
                     YIPIKFSVKGRDLASTIAEAQEKVKDPKSGAQLPPGYDIDWSGEFQQMEEANDRLMWI
                     VPISIGLILSLLYTAFSSIKDALLVMMNVVEASMGGILALWLTGTPFSISAAVGFVSV
                     FGVAVQDGVLLISYFNQLRAAGLPVRESVMRGAELRVRPVVMTSLTAALGLFPAAIAT
                     SIGSQAQKPLAIVVVGAMLCTLFLTRYLMPVLYSFFPAPAGKDECKAELILGSHYTDR
                     FLRPAAHSGHSAGQNDHGDGRGQSSTEYNGFGESQS"
     gene            937..2292
                     /locus_tag="VHS97_02615"
     CDS             937..2292
                     /locus_tag="VHS97_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447114.1"
                     /translation="MKISWKTLVAVIFIAAGAIGLALNGWTRGHVVGVLKQLSQAASS
                     GEESVSDKSWISKPRVPWDRTLTLEDYQIESIGLKTVAVKQQTEPTILKLFGLTDYDP
                     AKVTVIRTQFDSRVDSVLVDLGSTVKKGTPLLDLFSTDLAEAKSNYEAAISQWARDKK
                     VLDYKTPLAIGNNIPKKELIEVENDEAQSRLKMKLAKDKLLVYGLTDAEIENARNEDG
                     VQKAKMILRSRADGVVVLRSVVPGNYYTSADQLMTIAPLDHLWVRGSVSELDADQVQV
                     GQKVRIFFPSTNRTIDAHIDYIDKAIDPDSRSAKFRTTIPNPEGKLKAGMFVRTWPEI
                     PPMPGNTVIPRIAMVSVDRYDYVFVKKPGKANQYERRQIVVAKEHNDVVIVAKPSVGN
                     AGLVAGEEVVTTGSLILEQMYEDKVMTEGGLLAAGPEEDPTNSLNHPTPVVVTTTAAR
                     R"
     gene            2685..>3693
                     /locus_tag="VHS97_02620"
     CDS             2685..>3693
                     /locus_tag="VHS97_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3447115.1"
                     /translation="MNRKIGLTRSHAGPRRATKPANNIAWTVGLVFALAFWIWADRAK
                     AQAPTIDTSVPTLPGSGNSLLGQAPGLGGGSFLNLPGTGGILGGRAGVSAPKGIPTTI
                     STPATAAGPADMQMPISSPQPAPVSPTATPFYGTLEIVTQDDDGPPNGVTLEKAIDIT
                     LERSLDLHSKFLEIPMARADILQANLRSNPIFYQDGQLLQYKGTSTAFSRAAPGGPSQ
                     FDTNITYPFDISHKRQARTMVASRAERVLEALYQDAVRQRIDDIYGAFVTALAARQTV
                     RYAKTSADRLVEVTDRNEQRFKKGDISLADLNQVRIRLRTARLGLADAQATYRKTKLD
                     LG"
BASE COUNT          781 a         1106 c         1100 g          706 t
ORIGIN      
        1 catcggccgg atccccatcg acacgacggg cccggacggg aagccagggg cccgaatccc
       61 tcttcagcaa gtggtcaaca tcgaggctca caaacccggc gccgcgtaca tctatcgaga
      121 gaacaatcgc cgctacatcc cgatcaagtt cagcgtcaag gggcgcgacc tggcctcgac
      181 gatcgccgaa gcgcaggaga aggtcaagga tcccaagtcc ggcgcgcagc tgccgccggg
      241 atacgacatc gattggtcgg gtgagtttca acagatggaa gaagccaacg accgcttgat
      301 gtggatcgta ccgatctcga tcggactgat tctcagtctg ctttacaccg cattcagctc
      361 gatcaaggat gcactcctgg tgatgatgaa tgttgtggag gcatcaatgg gcggcatcct
      421 ggcactctgg ctgaccggaa ccccgttttc gatctcggca gcggtgggtt tcgtgtcggt
      481 ctttggagtg gcggtgcagg acggtgtgct gttgatctct tatttcaatc aattgcgggc
      541 ggcgggtctg ccagtgcgcg agtcggtgat gcgcggtgcg gaactgcgcg ttcgccccgt
      601 ggtcatgacc tcgctaaccg cggcgctcgg tctatttccg gccgcgatcg cgacatcgat
      661 cggctcgcag gctcagaagc cgctggcgat cgtggtcgtg ggggcgatgc tctgcaccct
      721 gttcttgact cgctacctga tgccggtgct gtatagcttc ttcccggctc ccgctggcaa
      781 ggacgagtgc aaggccgaac tgatcctggg ctcacactat acagaccggt tccttcgtcc
      841 agcagctcac tcgggccatt ccgccggtca aaatgaccac ggtgacggcc gcggccagtc
      901 gtcaacggag tacaacggct tcggagaatc tcagtcatga aaatcagctg gaaaacgctg
      961 gtggccgtca tcttcattgc cgccggcgcg atcggattgg cgctcaatgg atggacccgg
     1021 ggccacgttg tgggcgtttt gaaacagttg tcgcaggcgg cctcttccgg cgaggaaagc
     1081 gtttccgata aatcctggat ctcgaagccg agagtgccct gggaccgcac cctgacgctc
     1141 gaggactatc agatcgagtc aattggctta aagacggtcg cagtcaaaca gcaaaccgag
     1201 ccgacgatct tgaagctctt cgggttgaca gattacgacc cggcgaaagt gaccgtcatc
     1261 cgcacccagt tcgacagccg cgtggatagt gttctggtag atctgggctc gaccgtcaag
     1321 aaaggaactc ccttacttga tctcttcagc accgatctcg ccgaggccaa gagcaactac
     1381 gaggcggcga tcagccagtg ggcccgcgat aaaaaggtgc tcgattacaa aacccccctg
     1441 gccattggga ataatatccc gaaaaaggag ttgatcgagg tcgagaatga cgaggcccag
     1501 agccggctca agatgaagct ggccaaggac aagctgctgg tttatggctt gaccgacgcc
     1561 gagatcgaaa acgccaggaa cgaagacggc gttcagaagg cgaagatgat cttgaggtcg
     1621 cgggctgacg gggtcgtggt cctgcgatct gttgttccgg ggaactatta cacttccgcg
     1681 gatcaattga tgacgattgc gccgctcgac catctctggg tgcgcgggag cgtcagtgag
     1741 ctcgacgccg atcaggtcca agtcggacag aaagtgagga tcttctttcc ctctaccaat
     1801 cggacgatcg atgcccatat agattacatc gataaggcaa tcgatccgga ctctcgatct
     1861 gccaaattcc gcaccaccat tccaaatccg gagggaaagc tcaaggcggg tatgttcgtc
     1921 cgcacatggc cggagattcc gcccatgccg ggaaacaccg tgatcccgcg catcgccatg
     1981 gtctcggtcg atcggtacga ctacgtgttt gtcaagaagc cgggaaaggc caaccagtat
     2041 gagcgacggc aaatcgttgt tgcgaaggaa cacaatgatg tcgtcatcgt ggccaaaccg
     2101 tcagttggca acgccggact cgtagccgga gaggaagtcg tgacgaccgg cagcttgatc
     2161 ctggagcaaa tgtacgagga caaggttatg accgaagggg gcctgctcgc cgcgggaccg
     2221 gaagaggacc ccaccaattc tctcaaccat cctacgccgg tggtcgtgac gacaactgcc
     2281 gcccgtcgct gatcgcgtga cgtggctttc cgggtaaagc tggcataggg atcgacagcc
     2341 cgtctcgtcc gttctagcat tcgcgaccgg atcaccccct tcaccgtcac agtcttccca
     2401 attcgcacgc tcgcccggac ccggaaaatc cgcactgtgc gaatcgtacg tctttttcat
     2461 cgcccaagtt aagtcacatc cggtgcagtg gaccacgaca agtgaattcc ccctggtccg
     2521 gttgcgccga ttcgacaggc gatgatggtt cgtatgctcg cagcggacct tcagcttcgc
     2581 acttgagaca gccgatttac ggtggcagtg gttgttgcgg acgagctgaa tcgagcgaag
     2641 tgaccgattg aaacggggca cgacctgtgg gcacccatcc taggttgaat cgcaaaatcg
     2701 gcttgaccag gtcgcacgcg ggcccccgaa gagccaccaa gcctgcgaac aacatcgcgt
     2761 ggaccgtagg cctcgtcttc gccctggcct tctggatatg ggcggaccgc gcaaaggcac
     2821 aagctcccac gattgacacc agcgtgccca cgcttcccgg ttccggtaac tcgcttctgg
     2881 gtcaggcgcc cggtttgggc ggtggctcat tcctcaactt gccgggcacc gggggcattc
     2941 tgggcggacg cgcgggtgtg tcagcgccga agggcattcc cacgacgatc tcaacgcctg
     3001 cgacggcggc aggaccggcg gacatgcaaa tgcccatctc gtctccccag cccgcgccgg
     3061 tgtcaccaac ggcgaccccg ttttacggca cgctggaaat cgtgacgcag gacgacgacg
     3121 gcccgcccaa cggagtgaca ctcgaaaagg ccatcgacat cacgctggag cgcagcctcg
     3181 acctccactc gaagtttttg gagattccga tggcgcgtgc cgacatcctt caggccaatt
     3241 tgcggtccaa ccccatattt taccaagacg gtcagctcct ccagtacaaa ggtacaagca
     3301 cggcgttcag ccgcgcggcc ccgggcggcc ccagccagtt tgacaccaac atcacctatc
     3361 cgttcgatat ctcgcacaag cgccaggcac gcaccatggt tgcttcccga gccgagcgtg
     3421 tcctcgaggc gttgtatcaa gatgccgtcc gccagcggat cgacgacatt tacggagctt
     3481 tcgtcacggc gctggcggct cgtcagacgg tccgttatgc gaagacgagc gccgacaggc
     3541 tggtcgaggt cacagaccgg aatgagcagc gtttcaagaa gggcgacatc tccctggctg
     3601 atttgaacca ggtcaggata cggctgcgga ccgccaggct gggactggcc gatgcccagg
     3661 cgacgtaccg caagaccaag ctcgatctgg gat
//
LOCUS       DATAQL010000157         5375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_17716, whole genome shotgun sequence.
ACCESSION   DATAQL010000157 DATAQL010000000
VERSION     DATAQL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17716"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..675
                     /locus_tag="VHS97_02625"
     CDS             193..675
                     /locus_tag="VHS97_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447116.1"
                     /translation="MSRTPGVLMVMSVLCLAGCGSGHNIWVTGMLQKGGEMYKPPDGH
                     KLALYFCPMPGEGSATPTGDVEMADYDPRDGSFTVPGREGNGIPPGKYRIAVVETLRR
                     EALDQLKEASKSKNRGQQKLIRDDTNLLEASFGEKTSPFVRDLTTSTKLTLDMSKPNE
                     "
     gene            703..2700
                     /locus_tag="VHS97_02630"
     CDS             703..2700
                     /locus_tag="VHS97_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3447117.1"
                     /translation="MSGRSPAVCVHPLDSTTAIADQPISGLGCGETLTTRTRLGPWDV
                     LALAAWLGLAAGWLEVGTRVLCKSLVGTNRLYLTTRHFVWLVPLSNLLLFLIGGMLLA
                     AAAKLWPRLAGWLSPRLLCAAAVLPALMVAGPQVYPWAWVVVAAGISMRAVAWFERAD
                     FNSRRWLLFSFPGLLGSVIVVAGSVFGGDWLKERREAARSVPRAGTPNVLLIVLDTVR
                     ADRMSLYGYERSTTPALERLAQRGIRFAEARATAPWTLASHATMFSGRLPHDLKVHWQ
                     TPLRRDFPTLAEYLGSRGYATAGFVANTEYCSYDTGLDRGFTHYDDYPPDVKHLRPLR
                     TALFASSAWEAVSWGLWRSLGPLDATVRWLTDPGRKDAGSINRDFVDWLSHRQGSPRP
                     FFAFLNYLDAHVPYLPPEGTGFRYGTGPRTLEDFYVLVGGWKTIDKSRLPPNYRELIR
                     DCYDNCLSYLDRQLGELFDTLERRGVLDQTLVIVTSDHGEELGEHGLFEHGESLYRPE
                     IHVPLLIVLPARNQSPEVVRETVSLRDLTATIADLVGMADNAPFPGSSLARLRRDVRP
                     EGASRLRDDDGAISELSGPNPTSPSQGRSPAARGSLVSLAEEDYVYIRNEGDGREQLF
                     HESADPDEQVNLAKVEAMRPRLERLRRRLNQVRTNPPPAAR"
     gene            complement(2714..3802)
                     /locus_tag="VHS97_02635"
     CDS             complement(2714..3802)
                     /locus_tag="VHS97_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HEX3447118.1"
                     /translation="MLPTLRVIGSWFLLATAAMLGGDDGKIPTATPQAVVETEAVPSR
                     GDAADDPAIWIHPDNPAQSLVLGTDKKGGLNVFDLDGRRLQIVSDGARPNNADVLYGF
                     PLGDRPVDLVVAGTRSKARPGVAFWRIDPQNRRLTELGPLPAFTVFGGREPYGSCVYK
                     SHRDQSFYVFITSKDGDVEQYRLEADGQTAIRAQRVRSFNVGSTCEGCVADYELGWLY
                     IAEEKVGIWKFGAEPDADTSGTLIAKVGDHGLTADVEGLTIYYASDSKGYLIASSQGA
                     GTFQVYDRAGSNAFVLAIDPSSGTIGDVGETDGIDVTNVATSSRFPRGLFVCQDGQGK
                     NGFQNFKFFAWDQIAGDRLIVDTKRSAR"
     gene            4114..>5375
                     /locus_tag="VHS97_02640"
     CDS             4114..>5375
                     /locus_tag="VHS97_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3447119.1"
                     /translation="MDSDAIATDLRSIRNIGIIAHIDAGKTTTTERILYYTGEIHRMG
                     DVDKGNTTTDYREDERERGITIVAAAITCHWKDAEDKPITINIIDTPGHVDFTAEVER
                     SLRVLDGAVVVFSAVEGVEAQSETVWRQAAKYRVPRICFINKLDRIGAEFERVFEEIQ
                     ERLLESHPIAVQIPIGAGPEGTMGEFKGLIDLIAMKALFYKTEDLGSTITETEIPEDL
                     RPEAELWREKLLNALSNVDEIFTAAFMAHLEGAELPPAEIVAALRRATLTGLVHPILC
                     GSSLRYVGVQRLLDAVAAYLPSPLDKPPVVGHHPKKGTEITRKPDPNEPFCGLVFKIT
                     SDAHGDLSFVRVYSGRLKSGSRVYNPGKDKKEVCSRLYHIRADDREQIQESIAGDIAG
                     IVGLKDSVTGDTLCDGAHPILLERIEFP"
BASE COUNT         1069 a         1653 c         1613 g         1040 t
ORIGIN      
        1 gtcaagaact cgatcgcgtt tcccgtctgg ctcgcgatcc aggccaagaa cgacggcgtg
       61 gtcctcagct cggaccaata ttgatagaat cttacattgg cggccggtcg aaactgttgc
      121 gtagtctcga ccggccgcca agccctatct cggaccacat cggtctctcc tcttcccaat
      181 ccgagaatca tgatgagtcg aacacccggc gtgctaatgg tgatgagtgt cctttgcctc
      241 gcagggtgtg gcagcggtca caatatctgg gtaaccggca tgcttcaaaa ggggggtgag
      301 atgtacaaac ccccggacgg tcacaagctc gcactctatt tttgcccgat gcccggcgag
      361 ggatcggcta cgccaaccgg cgacgtcgaa atggcggatt acgatccgag ggatggcagc
      421 ttcacggtgc cagggcgcga gggtaatggc atcccccctg ggaaatatcg gatcgccgtt
      481 gtcgaaaccc tccgccgcga ggcactcgac cagttgaagg aagcctcgaa gtcgaagaac
      541 agaggacaac aaaagctgat tcgtgacgac acaaacctcc tcgaagcgtc gttcggcgag
      601 aagacatcgc cctttgttcg cgacttaaca acgtcaacca aactcacgct ggacatgtcg
      661 aaaccgaatg aatgaggatc tttctcagac agcaggtcct cagtgagcgg tcgttctccc
      721 gctgtgtgtg tacatccttt agactccaca accgcgatcg cggatcagcc gatatctggg
      781 ctgggatgcg gcgaaacgct cacgacccga acgcggctcg gtccctggga tgtgctcgca
      841 ctggcagcct ggctcggtct ggccgcggga tggctcgagg taggcacgag agtgctctgc
      901 aaaagcttgg tcggaaccaa tcggctctac ctcacgaccc gacactttgt ctggctcgtt
      961 ccgctgtcga acctgctgtt gttcttgatt gggggcatgc tgctggcagc agccgccaag
     1021 ctatggcccc gcctggcagg gtggcttagc ccgcggctcc tctgcgcggc ggccgtcctg
     1081 cccgcgctca tggtggccgg acctcaagtc tacccatggg cctgggtcgt agtcgcggcg
     1141 ggaatatcga tgcgcgcggt cgcatggttc gagcgggctg atttcaacag ccgccggtgg
     1201 ctcttgttta gctttccagg cctgttggga tcggtcatcg tcgtcgctgg ctccgttttc
     1261 ggcggagact ggctcaaaga gcggcgcgag gctgctcgtt cggtgccccg ggccggtacg
     1321 cccaacgtac tgttaatcgt gctggacacg gtgcgggcgg atcgcatgag cctctacggt
     1381 tatgagcgtt cgacaacgcc cgcactcgag cggctcgcgc agcgggggat tcgcttcgcc
     1441 gaggcgcggg cgaccgcgcc ctggaccctc gcttcgcacg cgacgatgtt ctccggccgc
     1501 ttgccccacg atctcaaagt ccattggcag acgcccctgc gcagggactt cccgacgctc
     1561 gctgagtacc tcgggtctcg gggctatgcg acggcgggtt tcgtcgcgaa taccgaatat
     1621 tgttcgtacg atacgggcct cgaccgcggc ttcacccatt acgacgacta ccctcccgat
     1681 gtcaagcacc tccgtccctt acggacagcg ctctttgcct caagcgcctg ggaggccgtg
     1741 tcctggggat tgtggcgaag tcttggcccg ctcgacgcca cggtccgttg gctcaccgac
     1801 cccggtcgca aggacgctgg atccataaat cgcgattttg tcgactggtt atcccaccgg
     1861 caagggtcgc ctcggccctt cttcgccttc ctcaactacc tggatgccca cgtgccctac
     1921 cttccgccgg aaggaaccgg gttccgctat ggcacggggc cccggaccct tgaggatttc
     1981 tacgtcctgg tcgggggatg gaagacgatc gacaagagcc ggctgccccc gaactatcgt
     2041 gaactcattc gagattgcta cgataattgt ctttcttatc tggacaggca actgggcgag
     2101 ttgtttgaca cattagagcg ccgcggcgtg ctcgatcaga ccctggtgat cgtcacctct
     2161 gaccatggcg aggaactggg ggagcatggt ctctttgaac acggcgaaag tctttatcgt
     2221 cctgaaatcc acgtgccgct gctgatcgtc ttgcctgcgc gtaaccagtc gccggaggtg
     2281 gtgcgcgaga ctgtcagctt gcgtgaccta acggcaacca tcgcagatct ggtcggcatg
     2341 gcggacaatg cgccgttccc aggttcatcg ctggcacggc tgcggcgaga tgtccggcca
     2401 gaaggcgcca gtcgccttcg tgatgatgat ggggcaatct ccgaactctc gggcccgaat
     2461 ccaacctccc cgagtcaagg ccggtctccc gctgcgcggg gttcgctggt ctcactggcg
     2521 gaggaagatt atgtctacat ccgcaacgag ggggacgggc gggagcagtt atttcacgaa
     2581 agcgctgacc ccgacgagca ggtcaacctc gcgaaggtcg aagcgatgcg cccccgactg
     2641 gagcgcctcc gacgacgtct gaaccaggtg aggacaaatc ctcccccggc cgcacgataa
     2701 actttgacag aatttaccga gcggagcgtt tcgtgtcgac gatgaggcga tcgcccgcga
     2761 tctggtccca cgcgaagaac ttgaagttct ggaagccgtt cttgccctgg ccgtcctggc
     2821 agacaaacag tccgcgcgga aagcgcgacg aggttgcgac gttcgtcaca tcgatgccgt
     2881 cggtctcacc cacatcaccg atcgtgccac ttgatggatc gatcgcgagt acgaaggcgt
     2941 tcgacccagc gcggtcgtaa acctgaaacg tgccggcccc ctggctcgac gcgatcagat
     3001 atcccttgga atccgaggcg tagtagattg tcaaaccctc gacatcggcg gtcagaccgt
     3061 ggtcgccgac ttttgcgata agagttcccg acgtgtcggc atcgggctcg gcaccaaact
     3121 tccagattcc gactttttcc tcggcgatgt acagccatcc cagctcatag tcggcgacgc
     3181 aaccctcgca agtcgagccg acattaaacg agcggactcg ctgggcacga atcgctgtct
     3241 ggccatcggc ctcgagccgg tactgctcga catcaccgtc cttgcttgtg atgaagacat
     3301 agaaactttg atcgcggtgg ctcttgtaaa cgcacgaacc atacggctcg cgtcccccga
     3361 agacggtgaa agccggtaac gggcctaact ctgtcaaacg gcggttttgg ggatcgatgc
     3421 gccagaacgc cacaccaggt cgcgccttgc tccgtgtgcc agccaccacc aggtcgactg
     3481 gccggtcccc gagcgggaaa ccgtagagaa cgtccgcgtt attgggacgc gctccgtcgg
     3541 agacaatctg taggcgccgt ccgtcgaggt cgaagacgtt caacccaccc ttcttgtctg
     3601 ttcccaaaac gagactctgg gcgggattgt ccggatggat ccagatggcc ggatcgtccg
     3661 ccgcgtctcc ccggctcggc actgcctccg tctcaaccac ggcctgaggt gtcgccgttg
     3721 gtatcttgcc gtcatcaccg cctaacatcg cggcggtcgc cagcaagaac caacttccga
     3781 ttacgcgtaa agtcggcaac acgcaagtgc tccttacgtt aactgaaggg aatcgacggc
     3841 gtccggccga aaacgttcgc tacaagagta aagccgacat cagctcctaa tatcaaatgt
     3901 tgttagggtg cattttttct taagggatgg ttaacgcgcg gtttcgttgg gcgatcacag
     3961 gagacataag ccgtacgcgc gacttgcgct gtaacgacgc ggcgcgtaag gtaagctgga
     4021 agaatcgaga ttcgccgcga gacgatcgca agcggccggt gtcggtgccg catgatcgag
     4081 ccgccgtttg aattgattag ggatgacgtg accatggaca gcgacgcaat cgcgaccgac
     4141 ttacgatcga tccgcaatat cggcattatc gcccacatcg atgccggtaa gactaccacg
     4201 accgagcgga tcctctatta cacgggcgag atccaccgca tgggggacgt cgacaaggga
     4261 aacaccacga ctgactaccg cgaagacgag cgggaacgag ggatcacgat cgttgcggcg
     4321 gcgatcactt gccactggaa ggatgccgag gacaagccga tcacgatcaa catcatcgac
     4381 acgcccgggc acgttgactt caccgccgaa gtcgagcgat cgttgcgagt actcgatggg
     4441 gccgtcgtcg tcttctcggc ggtggaaggg gtcgaggcgc agagtgaaac ggtctggagg
     4501 caggcggcaa agtatcgcgt cccgcgaatc tgcttcatca acaagctgga tcgcatcggt
     4561 gccgagtttg agcgggtgtt tgaggagatc caggaacggc tgctcgagag ccacccgatc
     4621 gccgtgcaga tcccgatcgg cgccggtccc gaagggacca tgggagaatt caagggactg
     4681 atcgacctga tcgcgatgaa ggcgctcttc tacaagaccg aggacctggg ctcgacgatc
     4741 accgagaccg agattccgga agatcttcgt cctgaggcgg aactctggcg ggaaaagctc
     4801 ttgaacgccc tgtccaacgt cgacgaaatt tttaccgcgg cattcatggc acacctggaa
     4861 ggggccgagt tgccgccggc cgagatcgtc gccgccctgc ggcgggcgac cttgaccggg
     4921 ctggtccacc cgattctctg cggctcgagc ctccgctacg tcggcgttca gcgcctcctg
     4981 gacgcggtcg ccgcttactt gcccagcccg ctggacaaac ccccagtcgt cggccatcac
     5041 cccaagaagg ggacggaaat cacgcgcaag cccgacccaa acgagccgtt ttgtggcttg
     5101 gtattcaaga tcaccagcga cgcccacggc gacctctcgt tcgtccgggt ctattcgggt
     5161 cgactcaaat cggggagccg ggtctataac ccaggcaagg acaaaaaaga ggtctgctcg
     5221 cggctttacc acatacgggc cgacgatcgc gagcagatcc aggagtcgat cgccggcgac
     5281 atcgccggca tcgtcggcct caaagactcg gtcaccggcg acacgctctg cgacggggcg
     5341 cacccgatcc tcttggaacg gatcgagttt cccga
//
LOCUS       DATAQL010000158         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_780062, whole genome shotgun sequence.
ACCESSION   DATAQL010000158 DATAQL010000000
VERSION     DATAQL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780062"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..631
                     /locus_tag="VHS97_02645"
     CDS             203..631
                     /locus_tag="VHS97_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447120.1"
                     /translation="MNRSRRIALTLPVAVAMSAIAGLGSLPAADDAKPPEKVIPAAHV
                     REHVGKECTAIMTVKSSKNAAPRKTYFLDSEEDFHDEKNLAVVISYDHADKFREAGID
                     DPAEYYRGKTIQVTGKVIEEDDQVRIRVEDPKQIKLVEPK"
     gene            651..1625
                     /locus_tag="VHS97_02650"
     CDS             651..1625
                     /locus_tag="VHS97_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3447121.1"
                     /translation="MSRHRGFTLIELLVVIAIIAVLIALLLPAVQSARAAARRIQCTN
                     NLKQLGLATLNYESTHGIFPPGQMKLTTKPPSGFTLFVNMLPFLEQQPLYNGWNFSNA
                     FDNLYGSTARSATIISALLCPADIIPQNPIQNGTANEWYGITSYGGNAGTQSHPFSAV
                     TSDGIFFYTGPAAPTFNQVRIAGVTDGLSNTLFFGERNHFDPNYDTFAAPGWTFFSQT
                     MAMWGWWASSSGGYGLSEVAMSTYASINYKIAFAYGSAAASGLSQSQFTTNYETPRVC
                     SFGSLHPGGANFAMGDGSVRFIKESIAQQAYHALGTRAGGEVVSADAY"
     gene            complement(1790..>2519)
                     /locus_tag="VHS97_02655"
     CDS             complement(1790..>2519)
                     /locus_tag="VHS97_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447122.1"
                     /translation="NGRPWAPKWLVDGIGVDYFGHVTQVTFVERGSDAELVHVGSFGR
                     IERLILSGSAVTDAGLADLKNLTRLQSLNLQGTKVTDAGLAHLGGLTSLQRLYLHNTQ
                     VIGTGLVHLKRLTDLQVLNVNNTPVNDTGLGYLAKATSLRELCLLGTQVTDAGLEHLD
                     GLHKLKVLDVPYTAVTDAGLEQLTNLPNLLDMRLDHTAISDGGLDKLKRLPLLQQLTL
                     GETKVTDAGVRGLKEALPKLRISR"
BASE COUNT          528 a          780 c          712 g          499 t
ORIGIN      
        1 cgccgctacg tttgccatga tcgtgtcaaa gaacgacagg attgtatcgg aaggcctcct
       61 ggcttagaat cacagaccgc gccatgattc tggcgcccca ggcttgggtg ataccccgaa
      121 gaagccgcat gatcgactcg acgcggcttc ttgctgaatg ggttcatcgt tcgtgctacg
      181 ccaacaatcc aggagagacg ccatgaatcg gtcccgccga attgccctca ctttgccggt
      241 cgccgtggca atgagcgcga tagccggcct aggctcgctc cccgcggctg acgacgcgaa
      301 gccgccggaa aaggtcatcc cggccgccca cgtccgcgag cacgtcggca aggaatgcac
      361 ggccattatg accgtcaagt caagcaagaa cgcggcgccg cgaaagacct attttctcga
      421 ttccgaggaa gacttccacg acgagaaaaa cctcgccgtc gtgattagct acgaccatgc
      481 cgacaagttc cgcgaagcgg gcatcgatga cccggcggaa tactacagag gcaaaacgat
      541 ccaagtgacc ggcaaggtga tcgaggagga cgaccaggtg cggatccgcg tcgaggaccc
      601 aaagcaaatt aagttggtcg agccaaagtg acccgcaaag aggatctcac gtgagcaggc
      661 accgtggttt tactctcatc gaactcttgg ttgtcatcgc tatcatcgcc gtgttgattg
      721 cgctcttatt gccggcggtt cagtccgccc gcgcggccgc gcggcggatc cagtgcacca
      781 acaacctcaa acagctcggc ctggccacgc tcaactacga atcgacgcac ggcatcttcc
      841 cgcccggcca gatgaaactc acgaccaagc ccccctcggg ctttacactc ttcgtgaaca
      901 tgctcccttt tctggaacag cagcccctct acaacggctg gaatttcagc aacgcgttcg
      961 acaaccttta tggctctacc gctcggtcgg ccacgatcat aagcgctctc ttgtgccccg
     1021 ccgacatcat tcctcagaat ccgatccaga acggcacagc caacgagtgg tacgggatca
     1081 cgagctatgg cggtaacgcc ggaacgcaat cgcatccgtt ttcggcggtg acctcggacg
     1141 ggatcttctt ctacacgggt ccggcagccc ccactttcaa tcaagtgagg atcgccggcg
     1201 tcaccgacgg cctctccaac acgctcttct tcggtgagcg caaccatttc gacccgaact
     1261 atgacacatt cgccgcaccg gggtggacgt tcttctcgca aacaatggcg atgtggggct
     1321 ggtgggcgtc gtcaagtggc ggctacggcc tctccgaagt tgcgatgagc acatatgcat
     1381 ccataaatta caagattgcg tttgcctatg gcagcgcggc tgcatcgggc ctgagccaaa
     1441 gccagttcac cacgaattac gaaacgcctc gagtgtgctc gttcggcagt ctgcatcccg
     1501 gaggcgccaa tttcgcgatg ggcgacgggt cggtgcgctt catcaaagag tcgatcgcgc
     1561 agcaggcgta ccacgccctg ggaacgagag ccggcggcga agtggtctcg gccgatgcct
     1621 attgacccat tgcgtggccc gagtgccgat cgcgccggtt cgcatgtctc gttctaatca
     1681 atggtgccgg cgattcccgc caaaatcatc cctccaacga caacccatgc tgctacaggc
     1741 atctcttctg ctccaaataa aggtcccaaa cgtcgcggga gagctccgat caacgggaga
     1801 tccgcaattt tggcaaagcc tctttgagcc ctcgcactcc ggcgtccgtg accttggtct
     1861 cgccgagggt caattgttga aggaggggca gcctcttcag tttgtccagc ccgccgtcgc
     1921 tgatggcggt gtggtcaagt cgcatgtcca gaaggttggg cagattcgtg agctgctcta
     1981 gaccggcgtc ggtgacagcg gtgtacggaa cgtccaatac cttcaacttg tgcaggccgt
     2041 ctaaatgctc cagcccggcg tcggtgactt gcgtaccgag gagacacagc tcccggaggc
     2101 tggtcgcttt cgccaggtac ccaagaccag tgtcgttgac cggtgtattg ttaacgttta
     2161 ggacttgaag gtcggtcagc ctcttcaggt gcacgaggcc agtaccaatc acctgggtat
     2221 tgtgaaggta aagcctctgg aggctggtca gaccgcccag gtgcgccagt cccgcgtcgg
     2281 tgaccttggt tccttggagg tttagcgact gcaggcgagt caggttcttc aagtccgcga
     2341 gccctgcgtc ggtcaccgcc gatccggaga gaatgagtcg ctcaatccgt ccgaaactcc
     2401 cgacgtggac aagttccgca tctgacccgc gctcgacaaa ggtgacctga gtgacatggc
     2461 cgaagtagtc gaccccgatg ccatcgacca accatttcgg agcccagggc ctgccgttc
//
LOCUS       DATAQL010000159         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4291684, whole genome shotgun sequence.
ACCESSION   DATAQL010000159 DATAQL010000000
VERSION     DATAQL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4291684"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..961
                     /locus_tag="VHS97_02660"
     CDS             422..961
                     /locus_tag="VHS97_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447123.1"
                     /translation="MNGFTRKPWRIGTQALFWSGSILIAVGSLGFGQAKAAPIHKVHE
                     LVDVDHKSYRTWADYVLGGPSVWSSLAHPPLTAGIEQAIWRNIRTDPSESNPMVQFFM
                     YRENLAPRRFDHYHPRLALELDKIVAAADAQQAKSSPPAPSSVQAQELGPAAQVPEPG
                     ILAIALMMTGYALWRRHRA"
     gene            1173..2759
                     /gene="metG"
                     /locus_tag="VHS97_02665"
     CDS             1173..2759
                     /gene="metG"
                     /locus_tag="VHS97_02665"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX3447124.1"
                     /translation="MGNESRFYITTAIDYPNSRPHIGTAFEKIGADVQARFRRMEGDT
                     VHFLMGNDENTIKVPQRARELGVEPKEYVDEMARQFQDVWRALDISYDDFIQTSEERH
                     HRGCQTFIQAVFDAGDIYKGVYTGHYCNGCEAFKTEKEVEEGGGRCPNHPNTPLTWVE
                     EENYFFRLSAYRDRLLAYYASHPEFIQPESRRNEVINLVESELKDVAITRKGFTWGIK
                     VPFDPAQTIYVWFDALLNYITAIGYGTDDGRFNSIWPANVHVIGKDITRFHCALWPAM
                     LMSAGLPLPLGVFGHGFVYRKNETTGEVQKLSKSLGNVIEPMELIEKFSADAFRYYFL
                     SQCPFGGDGEFSFERFADAYNSGLANNLGNLYSRILTMCLKYFDGHLEGAASVDVTQW
                     RKSLNPDELVGSLREQIGSFEYSAALQRIWRDVVDPANRYIQETQPFKLAKTDLAACH
                     TVLVNLADWLRVAAILIKPFLPRTAKTFYEAFNFEATNAWDQVGYGDAAVPQLPPDLQ
                     LMAPIVGGKPAPLFPKIDLG"
BASE COUNT          653 a          928 c          843 g          609 t
ORIGIN      
        1 gcagggattt cggtcgcgac cgctgtcgac atcgcgcatc tctccttcgg tcaatcagaa
       61 gcatctaatt caaatctggg taagcatttc cgtcttcagg ttgtttgcgc cggcgtgaac
      121 cagccttagg cggcatagtt cactcaaaga atacaggtcc cgacgctgac aatcaaccgc
      181 cccagaatct tgctctggct gcggcatgcg tcgcgggcac gcttcttctt ccgatttcct
      241 gccatttacg caatcttcgt aatcggtatt atccatggga tagataaagt cgagcacctt
      301 tgcataattc gctcaagctc tcgtacgtga ctgaccgaga atattcataa ggagcgcagc
      361 gctcgcgctc tcaatcccgc gagcatcagt acatgtagct ccgcttcctc gaaggtgacg
      421 catgaatgga ttcacgcgaa agccgtggcg gatcggaact caggctctct tttggagcgg
      481 atcaatcttg atcgccgttg gctccttagg tttcggacag gccaaggcgg ctccgattca
      541 caaggtccac gagctcgtgg acgtcgacca caaatcgtat cgaacctggg cagactacgt
      601 tctgggcggc ccgagtgttt ggtccagcct tgcacacccg ccgttgaccg ccgggatcga
      661 gcaagccatc tggaggaata tcaggaccga cccatccgaa tcgaacccga tggtgcagtt
      721 cttcatgtac cgggaaaact tggctccgcg acgctttgac cattaccatc cgagacttgc
      781 actcgagctg gacaaaatcg tggccgcggc agacgcccag caggccaaat cgtccccacc
      841 agctccctcg tcagtccagg cgcaagagct aggccccgcc gcacaggttc ccgagcccgg
      901 catactggcc attgcgctca tgatgaccgg ttacgccctc tggcggcgcc atcgtgcatg
      961 acgattggtt ccaacctgtc agaagtcggc acgtggcgat gcttcgccaa acgccgcaac
     1021 aacacttttg gtacattagt agtctcccga ggcgaggatg aacccggcgg gggggcagca
     1081 atgtctactt aactgacgcc gcgcgaatct ggaatcacaa cggcacacga aacgaaatcg
     1141 caccgagccc gccggcgatc gaggaaggaa cgatgggcaa cgaatcgcgc ttctacatca
     1201 cgacggcgat tgactatccc aacagccggc cgcatatcgg cacggccttc gagaaaatag
     1261 gggccgacgt tcaggctcga ttccgccgaa tggaaggaga tacggtccat ttcttaatgg
     1321 gcaacgacga aaacacgatc aaggtgccgc agcgcgcccg cgagctggga gttgagccta
     1381 aagagtacgt tgacgagatg gctcgccagt tccaggacgt ctggcgcgca ctggacatct
     1441 cgtacgacga cttcatccag accagtgaag agcggcacca tcgcggctgc cagacgttca
     1501 tccaggctgt attcgacgcc ggtgacatct acaaaggggt ttacaccggc cattactgca
     1561 acggttgcga agcgttcaag acggagaagg aagtcgagga aggaggcggc cgctgtccga
     1621 accacccgaa cacgcccctt acctgggtcg aggaagaaaa ttacttcttt cgactctcgg
     1681 cctatcgcga ccgactgctg gcatactatg cttcgcatcc ggaattcatt cagcctgaaa
     1741 gccggcgcaa cgaagtcata aacctcgtcg aatccgagct caaagacgtc gccatcaccc
     1801 gcaagggctt tacctggggg atcaaggttc cgtttgaccc cgctcagacg atctacgtct
     1861 ggttcgatgc cttgctcaac tacatcactg caatcggcta cggcaccgac gacggtcgct
     1921 tcaacagtat ctggccggcc aacgtgcatg tgatcggcaa agacatcact cggtttcact
     1981 gcgccctctg gccggccatg ctcatgtcgg cggggctgcc gttgccgctc ggcgtattcg
     2041 ggcacggctt cgtttatcgc aagaacgaga caaccggcga ggtgcagaaa ctgagcaaga
     2101 gcctgggcaa cgtcatagag ccgatggaac tgatcgagaa gttctcggcg gatgccttcc
     2161 gctattactt tctcagccaa tgcccctttg ggggcgacgg cgagttttcc ttcgaacgct
     2221 tcgcggacgc ctacaacagc ggcttggcca acaaccttgg caacctctac agccgcatcc
     2281 tgaccatgtg cttgaagtat ttcgacggcc acttggaggg tgcggcatcg gtcgacgtaa
     2341 ctcaatggcg caagagcctt aacccggatg agctggtcgg ctcgctgcgc gaacagatcg
     2401 gttcgttcga atacagcgcc gccttgcagc gaatctggcg cgacgtcgtc gacccggcca
     2461 accgctacat ccaggagacg cagccgttca agctcgccaa gaccgacctc gcggcctgcc
     2521 acacggtcct cgtcaacctg gccgactggt tgcgggtcgc ggcgatcttg atcaagccat
     2581 tcctgccccg cacggccaag accttctatg aagcctttaa cttcgaggca acgaacgctt
     2641 gggaccaggt cggatatggc gatgccgctg tgcctcagtt gccgccggat ctgcagttga
     2701 tggccccgat cgtcggcggc aagccggcgc ctctctttcc caagatcgac ctgggttgat
     2761 cttctcccca aaaagcgtcg gctacgactg gagcggccgc gttggtctgt cgagcaaaac
     2821 gctgttgacc gtccttggac ctccgcctat gatcccgatc gctagcgcgg aagccggccc
     2881 aaggtccacc gacggggatg attccgggaa acggatcagc ctcatggcaa tttcagattc
     2941 ggtcgtggcc aaaggcttct tgctcccctt gtgaaccgca agtgcgtcgt tctggccgat
     3001 cgggttgctc gtgacccgag ggaattacca cag
//
LOCUS       DATAQL010000160         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_933913, whole genome shotgun sequence.
ACCESSION   DATAQL010000160 DATAQL010000000
VERSION     DATAQL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933913"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..492
                     /locus_tag="VHS97_02670"
     CDS             55..492
                     /locus_tag="VHS97_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447125.1"
                     /translation="MRRSCLVFLSTVLMLAGGCSDQARDVGIKPPPSGGAMIRLPNGR
                     GFVAVKTELPAGSPKGKTKTRAATIVAVFYQTDGVTPMTPAPTDVVFKIGPPENAKSI
                     ALSPDAGDPSRFASLPGPYLSALQGIVHAKINGEDVEESFSAL"
     gene            complement(541..3078)
                     /locus_tag="VHS97_02675"
     CDS             complement(541..3078)
                     /locus_tag="VHS97_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3447126.1"
                     /translation="MKRLWISQVAAMVGAACLTLTYATTRQALAQDAPHYAGPTDKGF
                     LLPNGWKLQPAGEQVPQSDLPLNIVAMADNRHALVATSGYNSHDLSLVDLDQKRVANR
                     QTVRESWFGLAVSPEGDRVWWSGGGGSKVHAFRLADQRLANENGPEPETKDRTRVPKG
                     QGHFGGGIALDRNRKRLYSLDVDRGTLAALDLVDRKEVKSVPAGTRPYDVALARNGLQ
                     LYVSDWAGRTVRVLDAGDLRTVAKVAVGEHPNQIAVHPHDDRVFVACASSDSVSVIDA
                     TRGIVIETIHTALFPRSPEGSTPDALAIAPDGKTLFVANADNNNVAVIDIAAPSRSQV
                     KGFIPTGWYPTAVAVTPDGKRLLIGVGKGLQTKSNAIPPPLLGDAKAGSEAKARGEMT
                     REKTDRDPRHPHVGATLSGSLSIVTVPDDKTLAEYTETVYKNCPYSDKMLTDSPYPAQ
                     TAIPTKVGNPSPIKHVLYIIKENRTYDQVFGDMPRGNGDSSLVMFGREVTPNHHKLAE
                     QFVLLDNLYCNGHVSADGHPWSTMAYGTDYIARNWALTYSRRAGIDDDDDGDLSNAPS
                     GYLWDLCARNGRSYRNYGEYGRRVSQPDGSIKMEAAVPGLVGHMSPDYGISKVPGKRA
                     RDTDNADTFLREFREFEQNGNLPQLMIMSLGEDHTTGTRPGTFTPRACVASNDLALGR
                     IVDAITKSKYWPETAIFVIEDDAQNGPDHVDAHRTIGLVISPFTRRNYLDSTQYSTVS
                     MIRTFELILGLPPLSQYDAAARPMFDSFTDKADLATFTHEPPRIDLNEINEVTAYGAD
                     RSLKMDFDEYDKIDDFELNEILWRSIKGKDAPLPPAVRRAVAYRGGR"
     gene            complement(3270..3470)
                     /locus_tag="VHS97_02680"
     CDS             complement(3270..3470)
                     /locus_tag="VHS97_02680"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX3447127.1"
                     /translation="MTWYQIANDWQQFTAMVKEEWDKLTDDDLTTFGGQSDQLAGILQ
                     RKYGYAREQAEKEITEFSHKRR"
BASE COUNT          751 a         1112 c         1192 g          843 t
ORIGIN      
        1 tcaagggagc cagggagcaa tctctggctc gttattttta gggtgtttcc aaaaatgcgc
       61 cggagttgtc tcgtattcct gtcaaccgtg ctcatgctgg cgggcggttg ctccgatcaa
      121 gcccgcgatg tgggaataaa accgcctccc agcggaggcg ctatgatccg gttgccgaac
      181 ggtcgcggtt tcgtggcggt caagaccgag ctcccggccg gaagtccaaa aggcaagacc
      241 aagacccgcg cggccactat cgtcgccgtc ttctaccaaa ccgacggcgt caccccgatg
      301 acgccggcgc ccaccgatgt cgtgttcaag atcggtccgc ctgagaatgc gaaatcgatc
      361 gcactatcgc ccgacgctgg cgatcccagc cggttcgcct cgctgcctgg accctatttg
      421 agcgcactgc agggaatcgt gcacgcgaaa atcaacggcg aagatgtcga ggagtcgttt
      481 tccgcgctct gaccgagagc cgaggtgatc ggctcgtcgg tcgagggcgg ccacgcgcgg
      541 ctatcttccc ccgcgatagg cgaccgcgcg ccggacggcc gggggcagcg gggcatcttt
      601 tcccttgatc gaccgccaga gtatttcatt caactcaaag tcgtcgatct tgtcgtactc
      661 gtcgaagtcc atcttgagcg accggtcggc gccgtaggct gtcacttcgt tgatttcgtt
      721 cagatcaatc cgcggcggtt cgtgcgtaaa cgtcgcgagg tcggccttgt cagtgaacga
      781 gtcgaacata ggccgggcag ccgcgtcata ctgcgacaag gggggtagac ccaagatgag
      841 ctcgaacgtg cgaatcatgc tcaccgtgct gtactgggtg ctatcgaggt agttgcgcct
      901 ggtgaacggg ctgatgacaa ggccgatcgt ccgatgggcg tcgacgtggt cgggcccgtt
      961 ttgcgcatca tcctcgatca cgaaaatcgc cgtttccggc cagtatttgc tcttggtgat
     1021 ggcatcgacg atccgtccca acgccaggtc gttgctggcc acgcaggccc tgggcgtaaa
     1081 agtgcctggt cgtgtgcccg tcgtgtggtc ttctcccaaa ctcatgatca tgagctgggg
     1141 gagattgccg ttttgctcga actcgcgaaa ctcgcgcagg aacgtatcgg cgttgtcagt
     1201 gtcgcgtgcc cgcttgcccg gtaccttgga aatgccgtaa tccggcgaca tatggccgac
     1261 cagaccgggg accgctgcct ccatcttgat tgacccgtcc ggctggctga cccggcggcc
     1321 atattcgccg tagttacgat agctccggcc gtttcgcgcg cacaagtccc acagatagcc
     1381 cgacggggca ttggaaagat ccccgtcgtc atcgtcgtcg attcccgcac ggcgcgagta
     1441 agtaagagcc cagttgcgcg cgatgtagtc agtaccatag gccatcgtcg accagggatg
     1501 gccgtccgcc gagacgtgcc cattgcagta gaggttgtcg agcagcacga actgttccgc
     1561 cagcttgtga tgattagggg tgacctcgcg cccgaacatc acgagcgagg aatctccgtt
     1621 gccgcgcggc atgtcgccaa agacctgatc gtaggtccga ttctccttga tgatgtacaa
     1681 aacatgcttg atcggcgacg ggttgccgac cttggtcgga atcgctgttt gcgcaggata
     1741 tggcgagtcc gtcagcatct tgtcggagta agggcagttc ttgtacacag tctcggtata
     1801 ctcggcgagc gtcttgtcgt cggggaccgt gacgattgac agcgaacccg aaagcgtcgc
     1861 gccgacgtga ggatgtctgg ggtcgcggtc tgtcttctct cgcgtcattt ccccgcgggc
     1921 tttcgcctcc gatccggctt tcgcgtcgcc gagcagagga ggggggatgg cattggactt
     1981 ggtttgcagc cctttgccga cgccgatcag gagccttttg ccgtcggggg tgacggctac
     2041 ggcggtcgga taccaccctg tcggaataaa gcctttcacc tggctacggc tgggcgcggc
     2101 gatgtcgatc accgcgacat tgttattatc ggcattggcg acgaagaggg ttttgccatc
     2161 aggtgcaatc gcgagggcgt ctggcgtgct tccctcggga gaccgcggaa agagtgcggt
     2221 atgaatggtc tcgatgacga ttccgcgcgt cgcgtcgatg accgagacgg aatcgctcga
     2281 ggcacaggcg acaaagaccc ggtcgtcgtg aggatggaca gcgatctggt tgggatgttc
     2341 gcccacggcg actttggcga cggtgcgcaa gtcgcctgca tcgaggacgc gcaccgtgcg
     2401 gccggcccaa tcggaaacgt agagctgcaa tccgttgcga gccagtgcga cgtcgtacgg
     2461 ccgggtgccg gccggtacgg atttcacttc cttacgatcg acaaggtcaa gagcggcaag
     2521 cgtgccgcga tcaacatcga gcgaataaag ccgcttgcgg tttcgatcca aagcaatccc
     2581 gccaccgaaa tgaccctgtc ccttgggcac gcgtgttcga tccttggttt ctggttccgg
     2641 tccattctca ttggcaagcc gctgatcggc caggcggaac gcgtgcactt tgcttccccc
     2701 gccccccgac caccagacac gatcgccctc gggcgatacc gcaaggccaa accagctctc
     2761 acggacggtc tggcgattgg cgacgcgctt ctggtcgagg tcgacaagcg agaggtcatg
     2821 ggagttgtaa ccactggtgg cgacgagtgc gtggcgattg tctgccatcg caacaatgtt
     2881 gagcggcaag tccgactgcg gtacctgttc accggcgggc tgcaacttcc aaccgttggg
     2941 aagcaggaag cccttgtccg tcggtccagc ataatgaggg gcgtcctgag ccagtgcctg
     3001 gcgggtagtc gcgtacgtta acgtcaggca ggctgctcca accatcgctg ccacttgcga
     3061 gatccacagt cgtttcatga gagaggtcgc tccgtgtgcg ttcgtgatct ttgatccgag
     3121 attcaacgca caatagtcaa cgatcggacg cggccagtcc aatgagtttt tcgtgtaggt
     3181 ttggttgtct tgcccttcac gaacgcgaca atcacataag ccattaccgg tcaatttgtg
     3241 atgcccactt tacgcagtgc aaaccgctgt catcgtcttt tatgtgaaaa ttcggtgatt
     3301 tccttttcgg cttgctcgcg cgcgtagccg tattttcgct ggaggatgcc ggcgagttga
     3361 tcgctttgac cgccaaaggt cgtcaagtcg tcgtcggtta gcttgtccca ctcttccttc
     3421 accatggccg tgaactgttg ccagtcgttg gcgatctgat accaggtcat gcggcgacct
     3481 ccgggatcga acccatggtt aactcctcaa ggcctcaatc caccgtttca ttgtggcgcg
     3541 cctaacggaa atagcaagtg aattgtttgg agattatcaa tatggacata aaaggaaacg
     3601 tatatatgga atcaacacga agcgcaccgc caacacgaca gaggcggtac gcctgtgaac
     3661 atctcgtttg ggggcttcgg caaactgggc aatgcttcac gaagatttcc ctgtaatctt
     3721 gctgatatcg gcggttataa gaagacggtc ggaagaaggt ctggtgcgcc gctcgtggcg
     3781 ggcagatcaa ctagcgccga gcaaacgccg gccgacgagt tggtgatgtc aatttccgtc
     3841 agaggtcgct ggttcggtga ccggtgagac gatatcgatc cgtgaggaac tgagaatg
//
LOCUS       DATAQL010000161         4136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2460364, whole genome shotgun sequence.
ACCESSION   DATAQL010000161 DATAQL010000000
VERSION     DATAQL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460364"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VHS97_02685"
     CDS             <1..183
                     /locus_tag="VHS97_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447128.1"
                     /translation="RPLVNYNLSRDGSGDLGVGTDERGPWNDVGQWNFVAEPEESLKS
                     RVEGPKPGGKPIRTAK"
     gene            complement(194..988)
                     /locus_tag="VHS97_02690"
     CDS             complement(194..988)
                     /locus_tag="VHS97_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447129.1"
                     /translation="MIEDDAAGGEPALSAAIDQPPVREASGAVEAEETAAEKGWVIET
                     VVCPFHCLYQDALSFHTQSRLARSESEASRISRAALLLYVSSAEALVHQAAIELGRAE
                     LRGIIVDGERPLTLLEAWRVLPAIAAEPGTLVPTFDRESPPWPQFAELLALRTSWAYP
                     GPAAERRAFYRSARKDGDYEPLQLHQVPPSLRQAAGRNQLAFPRTGLPRDPYALRSHH
                     LDTARGILDAAIEALDRRMAGALTRDQRHRREPVRMVHPPSHEAAP"
     gene            complement(1282..2019)
                     /locus_tag="VHS97_02695"
     CDS             complement(1282..2019)
                     /locus_tag="VHS97_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3447130.1"
                     /translation="MPLRDHFRPPLADRRTWEGLHGGWPMMIVTGLNQKLPRRYIAEP
                     QVHLGSTIEIDVATYEEHDAGFSSAGETDEGGGVATAVWAPPLPTLAVASNLPDLDEY
                     EVRVYDTKRALRLVAAVEIVSPANKDRPEYRRAFVAKCAALLQDHVCVAIVDLVTTRS
                     ANLYGDLMELLGQTDPSLANEPPSLYAVACRGARKNEAWHLEMWTHTLALGTPLPTLP
                     LWLAENFAVPLELELSYEESCRVLRIP"
     gene            2163..3566
                     /locus_tag="VHS97_02700"
     CDS             2163..3566
                     /locus_tag="VHS97_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HEX3447131.1"
                     /translation="MNARGRLSRCVNAMFAVLFVLGLAPQAHAHFLFIRIGAPAEAGR
                     SAEVFFSEQAEAGDPKFVAKIAHTVLWIQTRPGEFRELPTNQAADRLRASLPSDRTLA
                     IVGKCEYGVLARPKETPFLLRYYPKAVAGNADDLNRLTPRLEIPFEIQPIFENQATDN
                     NKFGVSRIRLVALRNGKPSPHAVFTAVDSDLSEQTIKADSLGSATWTPAAPGRYSIYV
                     RETLKEPGVLGDKKYDEIREFATLALTWPLERHDADPNATALFKDAIAHRAEWRDFPG
                     FSADLNGHIDGRPFTGTVKVKNDGSVELQTDDPVAKPWLQDQLDSLVMHRIAQPSSES
                     ADAHQPRLRFADNDDDHPLGRLLAVDGDQMGSSYRIKDQQITVVNRRMGKQNMTITVL
                     DNDKNPDGRFLPHSYVVHYWDASTGKLNRVETFQERSRRVGSWDLPVLRSVVTASDSG
                     LVVKVVNLSKHALLDAR"
     gene            3573..>4136
                     /locus_tag="VHS97_02705"
     CDS             3573..>4136
                     /locus_tag="VHS97_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447132.1"
                     /translation="MTTTRPLPGDRGAMLPADRTDESARGWFVLVVWLGLTVVALGFV
                     TLYGNRTPRWEEWFLVPAVTGVQKVDLSWLWENVQGHRMPIFKLVLIACYAIFGFNSK
                     PILYLNVLLFSALSLGLLWAIRQVRGRWCHADAFLPIVLLNLGQTEAFSWAQTFTYVT
                     STCLETLLLILIVTHRGALNRTSIVLAG"
BASE COUNT          803 a         1260 c         1253 g          820 t
ORIGIN      
        1 cgaccacttg tgaattacaa tctatcgcgc gacggctcgg gcgacttggg cgtcggcacc
       61 gacgagcgtg gtccctggaa tgatgtcgga caatggaatt ttgtggccga gcccgaagag
      121 agcctgaaat cgagggtcga agggcccaag ccgggaggca agccgattcg caccgcgaaa
      181 taggcccggc gcgtcaaggc gccgcctcat gggacggcgg gtgaaccatg cggacgggct
      241 cgcggcgatg ccgctgatct cgcgtcaatg cgcctgccat gcggcggtcg agtgcctcga
      301 tcgcggcgtc gaggatcccg cgcgcggtat cgagatggtg ggaccgcagt gcataggggt
      361 cgcggggtaa tcctgtacga gggaaggcga gctggttgcg tccggctgcc tgccgcaagc
      421 ttggtggcac ctggtgcagt tgaagtggtt catagtcgcc gtccttgcga gcggaccgat
      481 aaaacgcccg ccgctcggcg gcgggccctg gatacgccca ggaagttcgg agcgcgagca
      541 actcggcgaa ctggggccaa gggggagact cgcgatcaaa cgttggcacc agagttcccg
      601 gctcggcagc gatcgcgggc aagactcgcc acgcttccaa aagcgttaac ggccgctccc
      661 catcgacgat gatcccccgc agttcggcgc gacccagctc gatcgcggct tgatgaacta
      721 gagcctcggc actcgacaca tacagtagaa gcgccgcccg cgagatccga ctcgcctcgc
      781 tttctgatcg cgccagccgg ctctgggtgt gaaatgagag ggcatcctga tacaggcagt
      841 gaaacggaca gaccaccgtt tcgattaccc agcccttttc ggcagccgtt tcttccgcct
      901 cgaccgctcc actcgcttcg cggaccggcg gctgatcgat agccgcggat agcgctggct
      961 ctccgccggc ggcgtcgtct tctatcatgg gccgtacagt tccatcaaat tcttatgcca
     1021 cttggaagcc acgccaacgg cggccggtgg tgacgacaac cggtctctcc cggttttttt
     1081 tgggccattt ggctgtcggc ggttgtcgag attgaagcag ataacctacc agattcgcga
     1141 gagcttccat agctttgagc attcccgggg atcaccacga ccaaacgaac cctgacacgc
     1201 agttcgtggt ccccgtgact tttgcgtggg gatgggacag ttcgccgtaa gcgtccgttt
     1261 cgcccagagg ggacacgacg tctacgggat gcggaggacg cgacaggact cctcgtagct
     1321 cagttccaac tccaagggga ccgcgaagtt ttcggcgagc cagagcggca gtgtcggcaa
     1381 cggcgtgccg agtgcaaggg tatgcgtcca catctccagg tgccaggcct cgttctttcg
     1441 ggccccgcgg caggcgactg cgtagagaga tggaggttcg ttcgccagcg atggatccgt
     1501 ctgccctaag agctccatca agtcgccgta caggttggcg gagcgggtcg tcacgagatc
     1561 gacaatcgcg acacaaacgt ggtcttggag taaggcggcg catttggcga cgaaggcgcg
     1621 acggtattct ggacggtctt tgttagccgg gctgacgatc tcgacggcgg caacgagccg
     1681 gagcgcacgc tttgtgtcat agacgcgcac ctcgtactcg tccaggtcgg ggaggttgga
     1741 tgcgaccgcc agcgtcggca aagggggagc ccagacagcg gtggcgacgc caccaccttc
     1801 gtcagtttcg ccggcagatg aaaatccggc atcgtgctcc tcgtacgttg ccacgtcgat
     1861 ctcgatcgtc gagcccaggt ggacctgtgg ctcggcgata tagcgccgcg gcagcttttg
     1921 gttgaggccc gtgacaatca tcatgggcca accgccgtgc aaaccctccc aggtcctgcg
     1981 gtcagcaagc ggcgggcgaa agtgatcccg taacggcatg acggaccctc ctaacgtaca
     2041 aagtaaccaa agggctgtgc ggctcacagc ccgttaccct gtaatggcag gtgctgacca
     2101 atcctgtaca atgatcagaa tgaatcgcaa acgcccgatt tgaccagacg gagatcttcc
     2161 caatgaatgc ccgcggtcgg ctttctcgat gcgtgaatgc gatgtttgcc gtcctctttg
     2221 tgctcggtct cgccccccag gcccacgcgc actttctatt catccggatc ggagcgccag
     2281 ccgaggccgg ccgatcggct gaggtctttt tcagcgagca ggccgaggca ggcgacccga
     2341 agtttgtcgc caagatcgct cacaccgtcc tttggatcca aacgcggccg ggcgagtttc
     2401 gcgagcttcc aaccaatcag gccgccgacc ggctccgggc ctcattgccg tctgatcgca
     2461 ccttggcaat cgtaggcaag tgcgagtacg gcgttttggc acgcccaaag gaaactccgt
     2521 tcctgctgcg ctattatccc aaggcagtcg ccggcaacgc cgacgatctg aaccgactga
     2581 cacccaggct agaaattcct tttgagatcc aaccgatttt tgaaaatcaa gcgaccgaca
     2641 acaacaagtt cggcgtgagt cgtatccgat tggtcgctct cagaaacggc aaacccagcc
     2701 cgcacgcggt attcacggcc gtcgattccg acctctccga gcaaacgatc aaggccgatt
     2761 cgctcggctc tgcgacctgg acaccggcgg ctcccggccg ttactccatc tatgtccgcg
     2821 aaacgctcaa ggaacccggc gttctgggag ataaaaagta cgacgaaatc cgcgaattcg
     2881 ccacgctggc attgacctgg ccgctggaac ggcacgatgc cgatcccaat gcgactgcac
     2941 ttttcaagga cgcgatcgca catcgtgcgg agtggcgcga tttcccgggc ttttcggctg
     3001 atctcaacgg ccacatcgac ggccggccgt ttactggcac ggtcaaggtc aagaatgacg
     3061 gctccgtgga gcttcaaacc gacgacccgg tcgcaaagcc ctggctccag gaccagctcg
     3121 actcgctggt catgcaccgg attgctcagc cctcgagcga atcggccgat gcgcaccagc
     3181 ccaggttgcg gttcgccgat aacgacgatg accatcccct cggccggcta cttgccgttg
     3241 acggggacca gatgggatcg agctaccgga tcaaggacca acagatcacg gtcgtcaatc
     3301 gccgcatggg aaagcagaac atgacgatca ctgtgctcga caacgacaag aaccccgatg
     3361 gccgattctt gccgcacagc tatgtggtgc attattggga tgccagcaca ggaaaactca
     3421 atcgggtcga gacttttcag gaacgctcgc gacgagtcgg ctcgtgggac ctgccggtac
     3481 tgcgttcggt tgtgacggct tccgattcgg ggctcgtcgt caaagtcgtc aatctttcca
     3541 aacacgcgtt actggatgcg agatgagcca tcttgacgac cactcgacct ctaccaggcg
     3601 atcgcggggc aatgctgccc gcggatcgga ctgatgagtc tgctcgcggc tggttcgtcc
     3661 ttgtcgtgtg gttgggcctg accgtggtgg cactcggttt cgtgactctt tatggaaatc
     3721 gcactccgcg ctgggaggaa tggtttctcg ttcccgccgt gaccggtgtc cagaaagttg
     3781 atctttcctg gctctgggag aacgtccagg gacacaggat gccgatcttt aaactggttc
     3841 tgatcgcgtg ctatgcgatc ttcggcttca actcgaaacc gattctctac ctgaacgttc
     3901 tgctgttctc ggctctgtct ctcggcctgc tttgggcgat tcggcaggtc cgcggtcgct
     3961 ggtgccacgc cgatgctttc ttgccgatag ttctcctcaa cctcggtcaa actgaggcgt
     4021 tttcctgggc gcagaccttc acgtacgtca cctcgacatg cttggagacg ctcctgctca
     4081 tcctgatcgt gacgcatcgc ggagctttga ataggacgag catcgtactg gccggc
//
LOCUS       DATAQL010000162         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4445870, whole genome shotgun sequence.
ACCESSION   DATAQL010000162 DATAQL010000000
VERSION     DATAQL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4445870"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VHS97_02710"
     CDS             <1..620
                     /locus_tag="VHS97_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX3447133.1"
                     /translation="AIAEADGEGETVIAATLARWLKVSAPAVTMAIRRLKRDGLIHVG
                     ADGEITLKKHGREIADRVLQRHHLIERMLTEIFGMEWYKVHEEAEQLEHAVSADFERR
                     LIEKLGQSESCPHGNQAGVDRPQDRRDRGWKPLDELETGSIARVMSVFERDRSLLEYL
                     DGLGIRPSAELQMVTRNYDDTLTLKVAGKPVQLGNTAAAKVWVGR"
     gene            808..1314
                     /locus_tag="VHS97_02715"
     CDS             808..1314
                     /locus_tag="VHS97_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447134.1"
                     /translation="MSRFAQTLSPAVSPWTPRQRPLQVEASIGRRKPAWAWARCLPLA
                     LLPVPFLIPLHSTPSPMVNLREELGQLHISWNVPTSSTLTILDGTDKTSILITPEHST
                     VTYARRSGDVTVKLGSALARFIGPLPPPPEIVQLRTSVHSLQSKIASLRKVQSADWAK
                     LSALQEYF"
BASE COUNT          352 a          569 c          478 g          329 t
ORIGIN      
        1 aggccatcgc cgaagccgat ggggaaggcg agaccgtcat cgccgcgacc ctggcccgct
       61 ggctgaaagt ttccgcccca gccgtcacca tggccatccg ccgcctgaag cgcgacggcc
      121 tgatccacgt cggcgccgac ggcgaaatta ccctgaagaa gcatggccgc gaaatcgccg
      181 accgcgtcct ccaacggcat catttaatcg aacgcatgct gacagaaatt ttcggcatgg
      241 agtggtacaa agtccacgaa gaagccgagc agttagagca cgccgtctcc gccgacttcg
      301 agcgccgcct gatcgagaaa ctcggccaga gcgagtcctg cccgcacggc aaccaggccg
      361 gagtcgaccg gccacaggac cgccgggacc gcggctggaa gccattggat gagcttgaga
      421 ccggctcgat cgcccgcgtc atgagcgttt ttgagcgcga ccgttcgctg ctcgaatacc
      481 tggacggcct gggcatccgt ccgtccgccg agctgcaaat ggtgacccgg aattacgacg
      541 atacgctgac cctgaaagtc gctggaaagc cggtgcaatt ggggaatact gcggccgcga
      601 aagtctgggt cggacgataa gggttccata gcgcgtaccg cggagtactc cccgcagcct
      661 tccgagcctt ccactaggta ttttcccagt tgtcgacccc catcgccgcc gctactcttg
      721 atcttgaact tacgaggctg atgcagtcgg agcagtaagc atcgaccctt cctttacagc
      781 agcttcacat ctaggtggat cacgcgtatg agccgtttcg cacaaacgct aagcccagcc
      841 gtcagcccgt ggaccccgcg gcaacgccct ctgcaggtcg aggccagcat cgggcgcaga
      901 aaaccagcgt gggcctgggc ccgatgcctg cctctagcct tgctgcccgt tccgttcctg
      961 atccctctcc actcgactcc gtcccccatg gtgaatctgc gggaagagtt gggacaactg
     1021 cacatttcct ggaacgtccc gaccagctcg actctgacga ttctcgatgg aaccgacaag
     1081 acgtcgattc taattacgcc ggagcattcg acggtgacct atgcccgccg ctccggcgac
     1141 gtgaccgtca aactcggctc cgcgctagcc cgcttcatcg gcccactgcc gcctccgccc
     1201 gaaatcgtgc aactgcgcac cagcgtccat tcgctgcaat cgaagatcgc atccctgcgg
     1261 aaggtgcaat cggccgactg ggcaaaactc tccgctctcc aggagtattt ctaagaggct
     1321 cggtcatttt ttgtggcgcg cgcgtccacg cgtgccgcgt cgacgtgcgg tagacacgag
     1381 tctacgaaat ctccgtctgg gtgcgataag tgctcaatca ccttagatcg caaaactttc
     1441 ccgccatttg cgcctaacct cgcgatgaac aaagcaatga acaaaatcta tctcgcccta
     1501 attctggctg gcgcggcgca tgccggttga ggttaaacgc ggaccgaatc ggtaggtgtt
     1561 cgcttgcggg attccgatca tcgtttgaga gacgccctac cggaaagcgc tgaacgtctg
     1621 ggtcgaccct gccggggccc cgacgaaccg actttagtac gttatcgccc cccaaacgcc
     1681 gcgcgttcgt tttggttgct cacaacccct taaaatacgc gaccgtct
//
LOCUS       DATAQL010000163         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_934429, whole genome shotgun sequence.
ACCESSION   DATAQL010000163 DATAQL010000000
VERSION     DATAQL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934429"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2728
                     /locus_tag="VHS97_02720"
     CDS             <1..>2728
                     /locus_tag="VHS97_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3447135.1"
                     /translation="PAAGLEATLWASEPLLVNPTNIDVDSRGRVWVAEGLNYRLTRGG
                     NKRFQRIEEADKIKILEDTDGDGKADKATVFADRVFPVPMGIAVEEHFDKSGKYQGCR
                     VFVGNSPDLLVLEDTDGDDKADKRYPLLTGFGGIDSDHGVHGMVLGLDGKLYFSHGDG
                     CCSVQQDHSERPQNFDVIDKSGRRVSSDQLANTLRVNRDGTQFEIICDRQRNNYETSL
                     NSFGNIFTSDNDDDGNRGCRVIWAMDGGHYGYRTVGSPRHWGEEVPGNVPKLVGTGNG
                     SPCGIMVYEGELLPPEYRGGIFEVDAGTRQVNFFPLTRKGAAFRTEYKVLLGGDDPWF
                     RPVDACAGPDGSVFVADWYDAGVGGHAFSDQTTGRIYRVAPKGHKSQNVKADFATPVG
                     LIAALKSPNIATQDAARRGLIARGREGNVGADVANMAVHDPDPLYRARALWIWHAKEG
                     DPIAMAALSEAVNQGDPRIREQAVRILGRDCRENGRVEYKNPDAKQPPAALKHVEILV
                     AMADDADAGVRRELILALRNLPTAKVGDALRKLATSWDGQDRWYLEALGLALEKREGD
                     YLSKLFDGKLYGNLDLERGGKENKVALPPYFPVDRNEAFIATGTPELPASAVTKYLGL
                     AWRLHRREVLPVLERFAPYLRAPELQQAVDDILERMSEPETADLVADMAARTTDSVHQ
                     QKLFLLLSHRLAGAWNLARDRPKVLQLIERAVSNPVTRAQGIELAAATGDARYRGTLE
                     VFAQDPEAPEDVRVAAVAAIGSFRMTPNRVLEHLVSSVRGKPSSNAVAEAAVRAMGEH
                     AGAQSRLTEILTGKDFPLGVRRQALRSMAQIRDGGSHVIELVRAGKLPEELKTEAATI
                     LHMSPDRRLRDEANKVLPLPKMASGRPLPSILQLVRSEGDVNKGRAVFFRAGA"
BASE COUNT          526 a          823 c          898 g          481 t
ORIGIN      
        1 gccggccgcg gggctggagg cgacgctgtg ggcatccgag ccgctgctgg tcaatcccac
       61 caatatcgac gtcgattcgc gcgggcgggt ctgggtcgcc gagggattga actatcggct
      121 cacgcgcggc ggcaacaaac gatttcagcg catcgaagag gccgacaaga tcaagatcct
      181 cgaagacacc gacggcgacg gcaaagccga caaggcgacc gtgttcgccg accgggtctt
      241 ccccgtgccc atgggcatcg ccgtcgaaga gcacttcgac aagtcaggga agtatcaagg
      301 ctgccgggtc ttcgtcggca acagccctga cttgctggtg ctcgaagaca ccgacggcga
      361 cgacaaagcc gacaagcggt acccgctctt gaccggattc ggtggaatcg actccgacca
      421 cggcgtgcac ggcatggtgc tgggcttgga cggcaagctc tacttcagtc acggtgacgg
      481 ctgctgctcg gtccagcaag atcactccga gcggcctcag aacttcgacg tgatcgacaa
      541 gagcggccgg cgtgtttcgt ccgaccaact cgctaatacg ttgcgcgtca accgcgatgg
      601 cacgcaattc gaaatcatct gcgatcgcca acggaacaac tacgagacca gcctgaattc
      661 gtttggcaac atcttcacca gcgacaacga cgatgacggc aaccgcggct gccgcgtgat
      721 ctgggcgatg gacggcggcc attacggcta tcgtacagtc gggagcccac ggcactgggg
      781 cgaggaagtg ccgggcaacg tgcccaagct tgtcggcacg ggtaacggca gtccgtgcgg
      841 catcatggtc tatgagggtg agctcttgcc gccggagtat cgcggcggga tcttcgaggt
      901 cgacgcgggt acccggcagg tcaacttttt cccgttgact cgcaaggggg ccgcattccg
      961 gaccgagtat aaggtgttgc tcggcggcga cgacccgtgg ttccggccgg tcgacgcgtg
     1021 cgccggtcct gacggctcgg tcttcgtagc ggattggtat gatgcgggtg tcggcggcca
     1081 tgcgttcagc gaccagacga ccggacgcat ctatcgtgtc gcacccaagg gacacaaaag
     1141 ccagaacgtc aaagccgact tcgccacgcc cgtcggcctg atcgccgcgc tcaaatcccc
     1201 caatatcgcc acgcaagatg cggccaggcg aggcttgatc gcgcggggca gggaaggcaa
     1261 cgtgggcgcc gacgtggcca acatggcggt gcacgacccc gacccgctct accgcgctcg
     1321 tgctctctgg atctggcacg cgaaagaagg cgatccgatc gcgatggccg cgctctcaga
     1381 agcggtcaat cagggggatc cgcgcatccg cgaacaggcc gtccgcatcc tgggccgcga
     1441 ttgccgcgaa aatggccgcg ttgaatacaa gaacccggat gccaagcagc cgcccgcggc
     1501 gctaaagcat gtcgagatct tggtcgcaat ggccgacgac gccgatgcgg gcgttcgccg
     1561 cgaactgatc ctggctttga ggaatctccc gacggcaaag gtcggcgatg cgttgcgcaa
     1621 gctggcgact tcgtgggatg ggcaagaccg ctggtatctc gaggccctcg ggctcgcgct
     1681 cgaaaagcgt gagggcgatt atttgtccaa gctttttgac ggtaagcttt atggaaatct
     1741 ggatctcgaa cgtgggggca aggagaataa ggtcgcgctt cccccctatt tcccggtcga
     1801 ccgcaacgag gcatttatcg cgacgggcac gcccgagttg cccgcgagcg ccgtcaccaa
     1861 gtatctgggg ctggcgtggc ggctgcatcg tcgtgaagtc ctgcccgtgc tcgagcgttt
     1921 cgcgccttat ctgcgagctc ccgagctgca acaggcggtc gacgacatcc tggaaaggat
     1981 gagcgagcct gaaacggcgg atctggtggc cgacatggcc gcgcggacga ccgattcagt
     2041 tcatcagcaa aagcttttcc tgctcttatc gcaccggctc gccggcgctt ggaatttggc
     2101 tcgtgatcgg cccaaggttt tgcaattgat cgaaagggca gtgtccaacc ccgtaacacg
     2161 tgcgcaggga atcgaactgg cggccgcgac gggtgatgcg cgctatcgtg ggacactcga
     2221 agtcttcgcc caggatcccg aggcacctga agacgtgcgg gtggcggccg tcgctgcgat
     2281 tggttcgttt cgcatgacgc ccaaccgggt tttggagcat ctggtgtcgt cggtccgggg
     2341 caagccgagc tccaatgctg tcgccgaagc ggcggtgagg gcgatggggg aacacgctgg
     2401 tgcgcagagt cggctcactg agatcctgac cggcaaggat tttccgctgg gcgtgcgacg
     2461 ccaggcgtta cgcagcatgg cccaaattcg agacggtggc tcgcacgtaa tcgagctggt
     2521 gcgcgcgggc aagctgcccg aggaactgaa aaccgaggca gccacgatct tgcacatgtc
     2581 gcccgatcgt cgcttgcgcg acgaagccaa taaagtcttg cctctcccca agatggcctc
     2641 cggacggccg ctgccgtcga tcttgcagtt ggttcgaagt gagggggatg tgaacaaggg
     2701 gcgcgcggtc tttttccgag ccggcgcc
//
LOCUS       DATAQL010000164         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3225197, whole genome shotgun sequence.
ACCESSION   DATAQL010000164 DATAQL010000000
VERSION     DATAQL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225197"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..459
                     /locus_tag="VHS97_02725"
     CDS             142..459
                     /locus_tag="VHS97_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447136.1"
                     /translation="MDATLAGPQSTPVSEPPQFKSRPGALAWAFRKSRDRWKAKCQEL
                     RTELKRHTNRVADLTKSREQWRSRAGATSAELETRDAEIAALRAEIAALELKKKSTQS
                     GRP"
     gene            483..1739
                     /locus_tag="VHS97_02730"
     CDS             483..1739
                     /locus_tag="VHS97_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447137.1"
                     /translation="MPRGQQYPLGVTATFLALVLRCATSLRGAAKVLELLGPLSDSEA
                     APHWSTGRLWLLRVGLAALELPKEIVDDRVWLVDHSIQIGTTKCLVILGVRPAQWSAG
                     RPLAHEDMELIALEPMASSNKLTVAECLRKAADRTGVPRAILDDHGADLHGGVNLFRG
                     DHPRVDEIYDVKHKVACLLRGHLEADPRWKAFTAELGPTTIRIQQTELAYLVPPSQRS
                     KARFMNIGMLMDWARMALVALDDPSPLRVEAERLRAKLGWLAGFRAEIADWSAAQAII
                     EATLDFVRCEGLYVGAGQDLTQRLPAASGTAGVLREELIDFVTRQSSQARIGERLPGT
                     TEVLESCFGRLKALEDGQSRSGFTGLVLSLGAMVGRLEPARLGEMLERVRVRDVMAWC
                     REKLGLSIQARRQEAYARAARATELG"
BASE COUNT          326 a          618 c          651 g          305 t
ORIGIN      
        1 agaggccgtc ccgcccggca ggcgggacct ggggtgacga aaggttgccc ttgacatccc
       61 cctctcgtct tccaaactat cagcgttttg ttgcgcggca gtcgtcgttc gcaccgcatt
      121 cacccaccgg agagccttgg catggacgct actctcgctg gtccccaatc cactcccgtc
      181 tctgaaccgc ctcaattcaa gagccgaccg ggcgcgttgg cgtgggcctt ccgcaaaagc
      241 cgcgatcgtt ggaaggccaa gtgccaggaa ctcaggaccg aactcaaacg ccacaccaat
      301 cgcgttgccg acctgaccaa gagccgagag cagtggcgca gccgggccgg ggccacgtcc
      361 gccgaactcg agacccgcga cgccgagatc gcggctctcc gcgccgagat cgcggccctg
      421 gagctcaaaa aaaagtcgac ccagtcgggt cgcccctgat ccccgagttc gccgtccccg
      481 acgtgccgcg cggccagcaa tatcccctcg gtgtcacggc gaccttcctc gccctggttc
      541 tgcgctgcgc cacatcgttg cgcggcgccg ccaaggtcct ggaactcttg gggcccctca
      601 gcgactccga ggccgccccg cattggtcga cgggccgact ctggctgctc cgcgtcggcc
      661 tggccgcctt ggaactcccc aaagagatcg tcgacgaccg ggtgtggttg gtcgaccact
      721 cgatccagat cggaaccacc aagtgcctgg tcatcctggg cgtccggcca gcgcagtggt
      781 ccgcgggacg gccgctggcg cacgaggaca tggaactgat cgcgttggag cccatggcga
      841 gctcgaacaa gctcaccgtg gccgagtgcc ttcgtaaggc cgcggaccgc accggcgtgc
      901 cgcgggcgat cctggacgac cacggggccg acctgcacgg cggagtcaat cttttccgcg
      961 gcgatcatcc ccgggtagac gaaatctacg acgtcaagca caaggtggca tgcttgctgc
     1021 gaggccatct tgaggccgat ccgcgatgga aagcgttcac ggccgagttg gggccgacga
     1081 cgatccgaat ccagcagacg gagttggcgt acctggtgcc gccgagccag cggtccaagg
     1141 cgcggtttat gaacattggc atgctcatgg actgggcgcg catggcgtta gtggccctcg
     1201 acgacccgtc gccgttgcgg gtagaggcgg aacggttgcg cgccaagcta ggctggctgg
     1261 ccggcttccg cgcggagatc gcggactggt cggccgctca ggccatcatc gaggcgaccc
     1321 tggatttcgt gcggtgcgaa gggctgtacg tcggggcggg gcaggacctg acgcagcggc
     1381 tgccggccgc ctcgggcaca gccggcgtgc tgcgggagga attgatcgac ttcgtgacga
     1441 gacaatcctc gcaagcgcgg atcggtgagc ggctgcccgg gacgaccgag gtgttggagt
     1501 cgtgcttcgg tcggctgaag gcgctggagg atggccagtc ccggagcggc ttcacggggc
     1561 tggtgctgag cctgggtgcg atggtgggga gactggagcc ggcgcgcctt ggagaaatgc
     1621 tcgagcgggt gcgagtccga gacgtgatgg cctggtgtcg tgagaagttg ggactctcga
     1681 tccaggcccg tcgccaggag gcttacgcga gggcggcgcg cgcaacagaa ttgggatgaa
     1741 ccgctcgggc gtgcaagccc aacttttcgt catcccaggc gggacctaca tgttgcggcg
     1801 aatcgaggag cgaacccatc gaacggggca agctcaggcc gcgtctcccc gctaaacacg
     1861 tacccgcgtg ggaacgagcg ggacgcacca gctccgcctc
//
LOCUS       DATAQL010000165         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2613976, whole genome shotgun sequence.
ACCESSION   DATAQL010000165 DATAQL010000000
VERSION     DATAQL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613976"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VHS97_02735"
     CDS             <1..1335
                     /locus_tag="VHS97_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3447138.1"
                     /translation="LSDNATSRAIMRHPDAVKTVGFSSDGLVALTVSSDQVRLWDAET
                     GEMLGAPLPHVKEVLDASFSPDGKAVLTRGRDSAIRIWSTAPIRSGERRLRHKGWVTA
                     VAFHPQGGESFLTGIGGSEAKVLSWSLRSDSQPDVTLNHLGPVLSLAYRRDGKVFAAG
                     TRDRRVWFGGEGRPSKLASLELDDRVWAVAFSPDGQTLLTGIEKGRAEFWDVATGKKE
                     LEALRHDKAVYAVAYSPDGRTILTGSEDMTARLWDTATRRPRGEPLLHQGTVYSVAFG
                     PPDGQVILTGSDDGTARLWQTSTGQPVGKPLQHPARVLAVAFSPDGRMVATGCGDGAA
                     RLWDATTGHPIGVPLLHHGPVRSVAFGAKATKTAEQIEGWTLLTGSEDMTARVWDIPP
                     PAVESPERIMRTLEAATGMVLDSQDVAETLRPAAWELLMRERNASTSSSGLR"
     gene            complement(1327..1755)
                     /locus_tag="VHS97_02740"
     CDS             complement(1327..1755)
                     /locus_tag="VHS97_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447139.1"
                     /translation="MARQEQDFNKETNQQSTTLFIKDSQGVTIDLTEVQCAQVLQSLL
                     TFFDLQGRLKPINQGSGRGPNIFSLLGRLPDAEMQDVEVNSLLQGQSIRIENCTDVSI
                     SVRQVEVENVVEIGLAMLRLLEQQVGGKTSLDRENLADLS"
     gene            2171..>3528
                     /locus_tag="VHS97_02745"
     CDS             2171..>3528
                     /locus_tag="VHS97_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3447140.1"
                     /translation="MVRDPNPPTSSSPGHDAPIAAGRPRQFSRSLRLVVSLLLLFATA
                     GGLVGWWIWWQFYVRTNNAYVAGNITPVSSEIGGMVVALYADDNMIVKAGDPIAQIDP
                     VQWQLAVDQDVADLKQLQAQERASDVGVRFIRRDRTALLEGTKARLAEADRAVRAAGV
                     EVQTRGRIRDKDRELLASTRARLPGLIASEENARGYFARFSRLAASGDVPVQDRDNRD
                     AAYREAMSKVESLRNEIAAAERQLLASELQLEETGVRLEQSQQARESARAAVGQAEAE
                     QLQPDIRVANLEAVRSQVRQAEAKLRTARVNLSHCLIRAPQGGIISRRTIQLGESLAA
                     KQPFLSIVPLDFDNVWVVANLREDQMDRVRAGNPAVVKLDAIPERSFLGWVESASGGT
                     GSVFSLFPPDNATGNFVRVVQRLPVRIRFAARENYQNRIRPGMSATVDIDHTNVVRRG
                     DRV"
BASE COUNT          669 a         1089 c         1129 g          641 t
ORIGIN      
        1 ctctcggaca acgcgacttc gcgtgcgatc atgcgccacc cagatgccgt caagactgtc
       61 ggtttcagct cggacggtct ggtcgcgctc acggtaagtt cagatcaggt ccgcctctgg
      121 gatgccgaga ccggcgaaat gctgggcgcc ccattgcctc acgtcaagga agtcctcgat
      181 gcgtcgttca gccccgacgg caaggccgtt ttgactcgag gccgagacag cgctattcgg
      241 atctggtcga cggcgccgat ccgttcaggc gagcgccgcc tcaggcacaa gggctgggtc
      301 acggcggtgg cattccaccc gcagggcgga gaatcgttcc tgaccggcat tggcggaagt
      361 gaagcaaaag tcctgtcgtg gagtttaaga tccgacagcc agcccgatgt cacgctcaat
      421 catcttggcc cggtcctttc attggcttac cgtcgggatg gaaaggtatt tgccgcggga
      481 acgcgcgacc gccgggtctg gtttggaggt gagggccgcc catcgaaact tgcgtcgctc
      541 gaactggacg accgggtctg ggcggttgcg tttagcccgg acggacagac tctgctcacg
      601 ggaatcgaaa agggccgcgc cgagttttgg gacgtggcca cgggtaagaa agagctcgaa
      661 gcgctccgtc acgacaaggc cgtctacgcc gtcgcctaca gtccggacgg gcggactatc
      721 ttgaccggta gcgaggacat gaccgcgcgg ctctgggaca cggccacgcg ccggcctcgg
      781 ggagagccgc tcctgcacca ggggacggtg tactccgtcg cgttcggccc gcccgacggt
      841 caggtcattt tgaccgggag cgatgacgga acagcccgtc tctggcaaac gtccaccggt
      901 caacccgtag gcaagcctct tcagcatcct gcacgggtgt tggcagtggc gttcagcccg
      961 gacggtcgga tggtggccac tgggtgtggc gacggggctg cccgtctttg ggacgccacg
     1021 accgggcacc cgatcggcgt cccgctcctt catcacggtc cggtccggtc tgtggctttc
     1081 ggcgccaagg cgacgaaaac agccgaacag atcgaaggct ggaccttgct caccggcagc
     1141 gaagacatga cagcccgagt ctgggacata cccccgccgg cggtcgaatc gccggaacgg
     1201 atcatgagaa cgctcgaggc ggccaccggt atggttctgg actcccagga tgtggccgag
     1261 actttgcggc ccgcggcgtg ggagctgcta atgcgggagc gcaatgcttc cacaagctcg
     1321 tcggggttaa gataaatcgg ccaaattctc tctatctaag gaggtttttc cgccgacttg
     1381 ctgctccagc aaccgcaaca tcgccaggcc gatctcgacg acattctcca cctcgacctg
     1441 cctgacgctg atgctcacgt ccgtgcagtt ctctatacga atcgactgcc cttgcagcaa
     1501 cgagttgacc tcgacatcct gcatttcggc gtcgggcagg cgccctagga gactgaaaat
     1561 gttcggtccc cgcccgctgc cctgattgat gggtttcagt cgcccctgga ggtcaaaaaa
     1621 cgtcagcaaa gactggagca cctgggcgca ctggacctcg gtcaggtcga tcgtgacgcc
     1681 ctggctgtcc ttgatgaaaa gtgtggtcga ttgctggttg gtctccttgt tgaagtcttg
     1741 ctcttgtcgc gccacggtac ccccctctga tcttgccatc ggagctgtga tgtcaccgct
     1801 cgaatcatac cagcgtcccg ggctctcaac ctcggcccgc aacgcgtccc cacttcgacc
     1861 ggtgcgtgat gcgcagggtc tcggaaaact gtgatgaacc cttttcgctc tcgcacgaag
     1921 ccggcaccaa ccgatcacac gagggaaaat tcagggaaat tgcggccgag atgtgcggaa
     1981 agcaggcaca aaccgccgaa tcattataat atggattatc ggattccgcc ggaggtgcgt
     2041 tcgatatttg atccgccaag atgaaagctg cctcacctcc ttcaaaaacg gagcagggaa
     2101 ggcaatgcat gatagcgccg cgtgaaccgg cgaggggcat ccccgtgttg aggaagagag
     2161 aagacccggg atggttcgag accccaatcc gcccacttct tcgagtccgg gccatgatgc
     2221 gccgatcgca gccgggaggc cacggcagtt ttcccgctcc ttacggctag tcgtttcgct
     2281 gttgctgctc ttcgccacgg ctggggggtt ggtcggctgg tggatttggt ggcagttcta
     2341 tgtccgtaca aacaacgcgt acgtcgcagg caacatcacg ccggtctctt cggagattgg
     2401 cggcatggtc gtcgcgctct atgccgacga caacatgatc gtcaaggcgg gcgaccccat
     2461 cgcgcagatc gaccccgtgc agtggcagct cgctgtcgat caggacgtcg ccgatctgaa
     2521 gcaattacag gcccaagagc gagcgtcgga tgtgggcgtg cgattcattc gtagggatcg
     2581 cacggcctta ctcgaaggta ccaaggcccg gctggccgag gccgaccgcg ccgtacgggc
     2641 ggccggtgtc gaggtccaaa ctcgggggcg catccgtgac aaggacaggg aactgcttgc
     2701 ctcaacgcgg gcgcgacttc ccgggctgat cgccagcgag gagaacgcca gaggctactt
     2761 cgcacgattc tcgcgactgg cagcctcggg tgacgtcccg gtgcaagacc gagacaatcg
     2821 ggacgcggcg taccgcgaag caatgtcgaa ggtcgagtca ttgcgaaacg agatcgcggc
     2881 cgccgagcgc cagttgctcg ctagcgagct gcaactcgag gagaccgggg tccgcctcga
     2941 acagagccag caggccaggg agagtgctcg agccgcggtg ggtcaggccg aggccgagca
     3001 gcttcagccc gacatccggg tcgcaaatct cgaggccgtc cgcagtcaag tgcgccaggc
     3061 agaggcgaag ctccgtactg cgagggtcaa cctgagccat tgcctgatcc gggctcccca
     3121 aggcgggatc atcagccggc ggaccatcca gctcggcgag agcctggcgg cgaaacagcc
     3181 gttcttgagc atcgtcccgc tcgatttcga caacgtctgg gtcgtggcga acctgcgcga
     3241 agaccagatg gaccgggtcc gggccggaaa cccggccgtt gtcaagttgg acgcgattcc
     3301 cgagcggtca ttcctcggct gggtcgaaag tgcctcgggt ggcaccggct cggttttctc
     3361 gctcttcccg cccgacaatg ccacgggcaa cttcgtgcgg gtcgtccaac ggctccctgt
     3421 gcggatcagg ttcgcggcgc gggagaacta ccagaaccgg atccgacccg ggatgtcggc
     3481 aacggtcgac atcgaccata cgaacgtcgt ccgccggggc gatcgagt
//
LOCUS       DATAQL010000166         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1850799, whole genome shotgun sequence.
ACCESSION   DATAQL010000166 DATAQL010000000
VERSION     DATAQL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850799"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VHS97_02750"
     CDS             <1..941
                     /locus_tag="VHS97_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEX3447141.1"
                     /translation="ANDYGVSELFANQQGKSFREIGKQTGIGFAPKSGMNVAFGDIFN
                     RGRFSVYVTNISEEGVLIQGNNLWVPREGTSGDSLKYDNLANDLGVELGGWSFGAQFG
                     DLNNDGNQDLILTNGYVSAAKGTSYWYEFSMVAGGNSTIISDAKNWPPMEGKSLSGYQ
                     HKRVWINDGAGHFTDVSQAVGYTDSHDGRGVALVDLWNRGVLDVVIVNQRGPVLLYKN
                     EVSPDNAWVDFELRGTRSNHSAIGAQVRLFWNGQEQLQQVSGGCGYSAQNQRRLHFGL
                     GKNTRIEKAFIRWPSGQEQTIESPAAGRVHKLEEPR"
     gene            938..1894
                     /locus_tag="VHS97_02755"
     CDS             938..1894
                     /locus_tag="VHS97_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEX3447142.1"
                     /translation="MTSPNSTVTTAEQNARPSAQRRGWSFDNRFLAPVLITGILLVGQ
                     LTFGILESYTRTLLAIVSSILMELVLARLITGKWPHLASAYITGISVGILIRSPALWP
                     YVLCSLISITSKYAIRWHGRHLWNPSNLGVSAMLYLAPASVAGLSVQWGNAIWPMLVI
                     WLLGSMIIMRLRRFHICATYVASFLFFSVARSAVTGDPWLAAVSPITGPMYQLFVFFM
                     ITDPKTTVRTKWGQCVVAFLVALVEAILRLNRVVHAPYYALFFVGPAANAIEIWWTSR
                     RTVPAAQQPLAVSSVTNSAPRVQSTADQPAALVTSGQADGNL"
     gene            1919..3292
                     /locus_tag="VHS97_02760"
     CDS             1919..3292
                     /locus_tag="VHS97_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX3447143.1"
                     /translation="MLANVERLVDLGESRESGLVGASLRAVEKAVQTADIARLQATDG
                     HFAAVARDEQTIRLGRTLGLPLRYFVAKRYHGPFLVVADRIDRLYESCCQHKIGWQFD
                     PLYTRMIPAHYLVEIDQVGCPDPNPRYHRFFTPEVGQGSGDVRELGTAYVRAAYEAIR
                     NWIAGVPDGEAIAILFSGGIDSGSVFLLARQALRELKRDPDLARAFTLDLGGGADADQ
                     ARRWVRELGLEACWEHVSLPGVKPNLEEAIATIEDYHPLDVECAATAFLLLKAIRRQY
                     PSLNYLLDGDGGDENLKAYPLEDSDLTISSVLKNPLLYQEGWGIEAIKHSLTFSGGLS
                     RAYVRTYAPAERLGFSAFSPYTTRSTIAAALAVPFERLLEGDAERLSWLKADIVREGV
                     ESVTGIRMPVFPKRRFQAGAGGDVYRNWRVSKAWCRQVFLRQWEERLGAAWDPAAERV
                     SGNAIPA"
BASE COUNT          644 a          995 c         1002 g          699 t
ORIGIN      
        1 ttgccaacga ctacggcgtc tccgagctgt tcgccaacca gcagggaaag tcgtttcgcg
       61 aaatcggcaa acaaaccggg attggcttcg cgcccaagag tgggatgaac gtggccttcg
      121 gcgatatctt caaccgggga cgattctccg tctacgtgac gaatatctcc gaggaagggg
      181 tcttgatcca gggcaacaac ctctgggtgc ccagggaggg gacctcaggc gattcgctca
      241 agtatgataa cctcgccaat gatctgggag tggagctcgg cggctggagc tttggtgctc
      301 agttcggcga cctcaacaac gacggcaacc aggatcttat attgacaaat ggctacgtct
      361 cagcggccaa agggacaagc tactggtacg agttttccat ggtggctggt ggcaacagca
      421 ccatcatttc ggacgccaag aactggcctc ccatggaagg caagagcctc tcgggttacc
      481 agcataagcg ggtctggatc aatgatgggg cagggcactt taccgacgtt tcgcaggcgg
      541 tcggctacac cgactcccac gacggccgtg gcgtcgctct ggtcgacctg tggaaccgcg
      601 gtgtccttga tgtcgtgatc gtcaatcaac gcggcccggt cttgctttat aagaatgagg
      661 tctcaccgga caacgcatgg gtcgatttcg agctgcgtgg gactcgttcc aatcatagcg
      721 ccatcggtgc ccaggtacgg ctgttctgga acggccagga acagttgcaa caggtcagcg
      781 gcggttgcgg ctattcggcc cagaaccagc gtcgcctcca cttcgggttg ggcaagaaca
      841 cgcggattga gaaggctttt attcgctggc cctcggggca ggaacagaca atcgaatccc
      901 ctgccgctgg ccgcgttcac aagctggagg aacctcgatg accagcccga actcaactgt
      961 gacgacggcc gagcaaaacg cccgtccctc cgcccagcgc cgcggctgga gtttcgataa
     1021 ccgcttcctc gcgccggtgc tcatcaccgg aatcttgctc gtcggtcagc tcacttttgg
     1081 aatcctggaa agctacacca ggacgctcct ggcgattgtg agcagcattc tgatggagct
     1141 ggttctggca cggctcatca ccggtaaatg gccccacctg gccagtgcat acattacagg
     1201 gatcagcgtc ggtattttga tccgttcgcc ggcgttgtgg ccgtacgtcc tttgcagcct
     1261 catttcgatc acatcgaaat atgccattcg ctggcacgga cggcacctgt ggaatccatc
     1321 gaacctgggc gtgagcgcca tgctttacct ggctccggca tcggttgccg gcttgagtgt
     1381 ccaatggggg aacgcgatct ggccgatgct cgtcatctgg ctcttgggct cgatgattat
     1441 catgcggctg cggcgcttcc acatctgtgc cacctacgtc gcgtcgttcc tgttcttttc
     1501 cgtcgcacga agcgccgtca cgggtgatcc ctggctggct gccgtgtcgc cgatcaccgg
     1561 gccgatgtac cagctcttcg tcttcttcat gattaccgat ccgaagacga cggtgcggac
     1621 caaatggggc cagtgcgtcg tggcattctt ggtcgcactc gtcgaggcca tcttgcgctt
     1681 gaaccgggtc gtccacgccc cctactatgc gcttttcttc gtgggcccgg cggcgaacgc
     1741 catcgagatc tggtggactt cgcggcggac ggttccggcc gctcaacaac cgctagccgt
     1801 gtcgagcgta accaattcag cgccccgcgt ccaatcgaca gccgatcaac ctgccgccct
     1861 cgttacgtcg ggtcaagccg atggaaatct ctaaggtctg aatatgggag aaccttaaat
     1921 gttggccaat gtcgagcgcc tggtcgatct gggggaatca cgcgagtcgg gactggtggg
     1981 ggcatcttta agggccgttg aaaaagccgt gcaaaccgca gacattgcca ggttacaagc
     2041 gaccgatgga cactttgctg ccgtggcccg cgatgaacag acaattcgtc tgggacgaac
     2101 cctcggcctg ccgctccgct acttcgtggc gaaaagatac cacggaccgt tcctggtcgt
     2161 ggccgatcgg atcgaccggc tctatgagtc ttgctgccag cacaagatcg gctggcagtt
     2221 tgatccgctc tacacgcgga tgatccccgc acattacctt gtggagatcg atcaggtcgg
     2281 ctgtcccgac ccaaatccgc gttaccatcg gttttttacg cctgaggtcg gccaaggctc
     2341 tggcgatgtt cgcgagctcg gcacagctta tgtgcgcgcc gcctacgagg cgatccggaa
     2401 ctggatcgcc ggggtgccgg acggtgaagc gattgcgatc cttttctccg gcggaataga
     2461 cagtggctca gttttcctgc tggcgcgaca ggcactccgc gagctgaagc gagacccaga
     2521 tctggcgcga gcgtttactc tcgacctggg gggtggcgcc gatgccgacc aggccaggcg
     2581 ctgggtgcgt gagctagggc tcgaggcctg ctgggaacat gtttctctac cgggagtaaa
     2641 gcccaacctg gaagaggcca tcgcgacgat cgaagactat catcccctgg acgtcgaatg
     2701 cgcagccacc gcgtttttgc tcctgaaggc gattcgtcgc caatacccgt cgcttaacta
     2761 cttgctcgat ggtgacggcg gcgacgagaa cctgaaggcc tatccgctcg aagactcgga
     2821 ccttacgatt tccagtgtgc tgaagaatcc ccttttatac caggaaggct ggggcatcga
     2881 ggcgatcaag cacagcctta ccttttcggg tggtctctcg cgtgcttatg tccgcaccta
     2941 tgcccccgct gagcgactcg gcttttcggc ttttagcccc tacaccaccc ggtcgacgat
     3001 cgcggctgcg ctcgcggtcc cgttcgagag attgctcgaa ggagacgcag aacgcctctc
     3061 ctggttgaaa gccgacatcg tccgcgaagg cgtcgagtcg gtaacaggga ttcggatgcc
     3121 cgtatttccc aagcgacggt tccaggcggg ggcaggcggc gacgtctacc gcaactggcg
     3181 agtcagcaag gcctggtgcc gccaagtctt cttgcgccag tgggaagaga ggctgggcgc
     3241 agcatgggac cccgcggccg aacgagtctc gggcaatgcc attccagctt gaaacacggg
     3301 atcttggaat tggctgacaa ccctccacaa gcggttgact
//
LOCUS       DATAQL010000167         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1698370, whole genome shotgun sequence.
ACCESSION   DATAQL010000167 DATAQL010000000
VERSION     DATAQL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698370"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VHS97_02765"
     CDS             complement(<1..900)
                     /locus_tag="VHS97_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3447144.1"
                     /translation="MPGTWQIGDRIADRFDVFEVHHGGMGVVYLVLDRLRTVGHETVA
                     LKTLHDEFLPDHVRLARFAEECRLWIQLGRHPHIVQALAVEEIGGKPYIVLERATGGE
                     LRSRIGTQPLDPIQALRHGVEFCLGMEHAIRQGLRCHRDIKPGNLLISGSGALKITDF
                     GLAGIRDEFLSSIDWPDAPIPLAEATVQQPIVWTDPRDQPGPIRSSPTDATTGDGSVG
                     SGTTEPYRDAGPDLQRADTSRLAIGSTSHEDPNAFATIEYTPPGRSNLRDAPRSRLTR
                     TGVMLGTLPYMAPEQFRDSKAVDV"
     gene            1492..>1929
                     /locus_tag="VHS97_02770"
     CDS             1492..>1929
                     /locus_tag="VHS97_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447145.1"
                     /translation="MFAIPILLGLVLTAPADESALGAARLPATDAAILDFFQKRSQPP
                     PARTAIEQLARALGSQNATEADDAQGKLVSIGAPVVPVLREVANRVDAVRTSQRAKEV
                     LQLIEGPRAALLPVEAAQLLAARKPPGTAEALLGYLPFAENDAV"
BASE COUNT          326 a          636 c          634 g          333 t
ORIGIN      
        1 gacgtcaacg gccttggagt cgcggaactg ctcgggggcc atgtagggaa gcgtccccaa
       61 catcaccccg gttcgcgtca gccgcgagcg aggggcgtca cgcagattag agcggcccgg
      121 cggggtgtat tcgatcgtcg caaaagcgtt cggatcctcg tgcgatgtgc tgccgatcgc
      181 caagcgtgag gtatcagcgc gctgaaggtc cgggccggcg tcgcgatacg gttcggtcgt
      241 tcctgatcca acagagccgt ctccggtcgt cgcgtccgtg ggcgacgagc gaatcggccc
      301 cggctgatct cgggggtctg tccacacgat cggctgctga acggtcgcct cggccagggg
      361 gatcggtgcg tccggccagt cgatgctgga gaggaactcg tcacgtatgc cggccaggcc
      421 gaagtcggtg atcttgagcg cgcccgaacc ggagatgagg aggttgccgg gcttgatatc
      481 acgatggcac cgcagaccct ggcggatcgc gtgttccatg cccaggcaga actcgacgcc
      541 gtgccgcagt gcctggatcg ggtcgagcgg ctgagtcccg atccggctcc gcaactcgcc
      601 gccggtcgcc cgttcgagca cgatgtaggg tttgcccccg atctcctcga ccgcgagcgc
      661 ctggacgatg tgcgggtgcc gtcccaattg gatccagagt cggcattcct cggcgaatcg
      721 cgcgagccga acgtggtcgg gaaggaattc gtcatgcagc gtcttcaatg cgacggtctc
      781 atgccccact gtcctgaggc ggtccaggac caggtagacg acgcccatgc cgccgtgatg
      841 gacctcgaac acgtcgaacc gatcggcgat ccgatcccca atttgccagg tcccaggcat
      901 gatgggtcgc ctcctccaga accctcgatc gtcctgttgt acgagaagcc tcccgttgcg
      961 ctgccgtgct ctccatgcta acgagcctgc gtcgcggagg cgcacagaac gccggcgcat
     1021 tgcgtgtgtt ggggcgagtt tcgggtattt taccgtgcga ctagagggta gccggggggt
     1081 ggcgtccctg ctacgatacc cggcggtacg ttaccggagg acgccatggc catcctcgaa
     1141 cacctcgacg cccgaacccg gctcgccggc gccttcgagc cggcccacca gacccatcga
     1201 ggctctccag ggccacccgc ggcagcatcg gcgtcacgat cgacccggtt cgacaacgcc
     1261 cgccaatgtg tcaacgacag ggccggacgc cgccatctga agcccggtgc cgcggaaatg
     1321 atggcatctg ttcgaggggt tcggaacgca tctatactgg cagttgtgct tgatcgaaac
     1381 gcgcggcgaa ctgtccgatc cgacctttca ggtgggacgg gctggcgatc ccaccgcgaa
     1441 tgaccacgaa gcaagagcat ccggccaaac tgtttccgca ggaggtcgcc aatgttcgcg
     1501 atcccaattc tcctggggct agtcctaaca gctcccgcgg atgaatcggc tctgggggcc
     1561 gctcggttgc cggcgactga cgcggcaatt ctcgacttct tccagaaacg ctcccagccg
     1621 ccgcctgcgc ggactgccat tgagcagctc gcgcgggccc ttggcagcca gaacgcgacc
     1681 gaggccgacg acgcccaggg aaagctggtc agcatcggag cccccgttgt ccctgtgctc
     1741 cgcgaggtcg ccaatcgggt ggatgccgtc cgcacttccc aacgggcaaa ggaggtcttg
     1801 cagctgatcg aggggccgcg cgccgccctt ctgcccgtcg aggccgccca attgctggcc
     1861 gcgcggaaac cgcctgggac cgccgaggcg ctgctgggct acctgccctt tgcggaaaat
     1921 gacgcggtc
//
LOCUS       DATAQL010000168         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1850841, whole genome shotgun sequence.
ACCESSION   DATAQL010000168 DATAQL010000000
VERSION     DATAQL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850841"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1489
                     /locus_tag="VHS97_02775"
     CDS             446..1489
                     /locus_tag="VHS97_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3447146.1"
                     /translation="MISIDFATEQIEALHHERFHHPHPRVQERMEAVYLKSQGLPHKD
                     ICRLSRISENTLRAYLRQFQEGGIERLKRLDFAVPQSELADHRDTLEEYFRKNPPRSA
                     AQAADDIERLTGVRRGATQVRKFLDGMGMKCRKLGMIPAKADADEQSKFLDEKLWPRL
                     KQAQRLKRVVCFVDAAHFVHGPYLGYLWCFVRLLMRGPSGRKRFNVLGAIDAVTHELT
                     TVCNETTINARAICELLGKLSARYVGLPMTLVLDNARYQKCAVVQALAVSLKIELLFL
                     PPYSPNLNLIERLWKFVKKECLSCRYHEDFARFKAAIVDCLEGVEGKHKGAIQSLITL
                     KFQTFDNPQILAA"
BASE COUNT          319 a          503 c          503 g          293 t
ORIGIN      
        1 tcggcgccca ccgccgccat cgcggcccgg agctgcggca gatccagggc gtcgagcggc
       61 gggaccgggg gtctaacaat catcggacca ggaactcggg ataaggccca aatcgctgcc
      121 aggtcaagcc ttacggccgg cattgcgtgc ttgaatttgg atcggaatcc tcacgatcca
      181 tccctgtgat aaggatgatt atcacgggaa tcccggaccc gacacgcggt aatctccggc
      241 agaaatgacg cggtccgatc gctgaaactt catcgttatc acaggaaccc ggggatccgc
      301 tctgcaaggt cgttcgctgg aggtcagaag cttataccta tcgcggtccg agtttgaggg
      361 ttgtttcggc tgttcaggcc ttctacactg accgatgctt tcaagcatcg ggacacagac
      421 accgctcccc cagccggtca ccgcgatgat ctccatcgac ttcgctaccg aacagatcga
      481 ggccctccac cacgaacgtt tccatcatcc ccatcccagg gtccaggaga ggatggaggc
      541 agtctatctc aagagccaag gcctgcccca caaggacatc tgccggctct cgcggatctc
      601 cgagaacacg ctgcgggcct acctccgcca gttccaggag gggggcatcg agcggctcaa
      661 gcggctcgac ttcgccgtgc cccagagcga gctggccgac catcgcgaca cgttggagga
      721 gtacttccgg aagaatccgc cccgatcggc cgcccaggcc gccgatgaca tcgagcggct
      781 caccggcgtc cgacgcggcg cgacccaggt ccgcaagttt ctcgacggga tggggatgaa
      841 gtgtcgcaag ctgggcatga tcccggccaa ggccgatgcc gacgagcagt cgaagttcct
      901 cgacgagaag ctctggcccc gcctgaagca ggcccagagg ctcaagcggg tggtctgctt
      961 cgtcgacgcg gcccacttcg tccacggacc gtacctcggc tacctctggt gcttcgtccg
     1021 gctgctgatg cgcggcccct ccggtcgcaa gcggttcaac gtcctggggg cgatcgacgc
     1081 ggtgacgcac gagctgacga ccgtctgcaa cgagacgacg atcaacgccc gagcgatctg
     1141 cgagctgctc ggcaagctgt cggcgcggta cgtggggctg ccgatgacgc tggtgctgga
     1201 caacgcccgt taccagaagt gcgccgtggt gcaggcgctg gcggtgtcgt tgaagatcga
     1261 gttgttgttc ctgccgccgt actcgccgaa cctgaacctg atcgagcggc tgtggaagtt
     1321 cgtcaagaag gaatgcctgt catgccggta tcacgaggat ttcgcacgat tcaaggcggc
     1381 gatcgtggat tgcctggagg gagtcgaggg taagcacaag ggggcgatcc aatcgctaat
     1441 cacgctcaaa ttccagacct tcgacaatcc tcagattctg gccgcgtgaa gtatagtcac
     1501 cgcgccactc ctcgactcta caaactcaat gaatcccgat tttcccggga cggtggcgcc
     1561 ggggacgtcg ctcagggact tattcggatg gtggttcgag gagaggaggg gtcgaatc
//
LOCUS       DATAQL010000169         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3683030, whole genome shotgun sequence.
ACCESSION   DATAQL010000169 DATAQL010000000
VERSION     DATAQL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683030"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VHS97_02780"
     CDS             complement(<1..1242)
                     /locus_tag="VHS97_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447147.1"
                     /translation="MAIFKNCESTTRRDCLRLGLSALIGGGLIDALRIRAQAAIPNIR
                     PTSCILIWMDGGPSHYETFDPKPGAPAEIRGKFGPIATKVPGIQFSEPMKRLAGIADK
                     IAIVRSIRHDQGNHGAGNHYMMTGAPTRIPVGCGAFVSFHPSMGAVTAHERGTTGGLP
                     PYFSIPSMTRSGGPNFLGARYAPFVVPDDPNAASFRVRDVAPPRGLADNRVDGRRDLR
                     DRLDHFRRYADKAAGDPALALDEYYDQGYDLMASRQAQSAFDITREDPKVRDRYGRSP
                     FGQRCLLARRLVEAGVPFVTLNEGGWDHHVSLFDKFDQRMPAVESSIATLIDDLDQRG
                     LLDSTLVIALGEFGRTPKINKDAGRDHWSNAMSVLVAGCGTPGGQVVGATDARGYAAS
                     DRVLSPENFAATVYTKLGINPG"
     gene            complement(1300..>1692)
                     /locus_tag="VHS97_02785"
     CDS             complement(1300..>1692)
                     /locus_tag="VHS97_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3447148.1"
                     /translation="PPRSRALEIWTNRVPSLFLDTFGRPDPNQDPPCERTSDTAVVQA
                     LHLMNSAGVHTKLSADRGRVARLAKSDKAPHAIVEELYLLTYSRRPTREEASVALALF
                     ADPKSDRRGAVEDLLWALLNSPEFVFKD"
BASE COUNT          311 a          503 c          563 g          315 t
ORIGIN      
        1 accaggattg atcccaagct ttgtatagac ggtggccgcg aagttctcgg gggaaaggac
       61 gcggtcactg gcagcatatc ctcgggcgtc cgtcgcaccg acgacctggc cacccggagt
      121 accgcaacca gcgaccaata ccgacatggc gttcgaccag tggtctcggc ccgcatcctt
      181 gttgatcttc ggtgtccggc cgaactctcc gagtgcaatc accaaagttg aatcgagcaa
      241 tccgcgctgg tcgaggtcgt cgatcagggt cgcgatcgaa gactcgaccg caggcattcg
      301 ctgatcgaat ttgtcgaaga gcgaaacgtg gtggtcccag ccaccttcat tgagagtgac
      361 gaacgggaca ccggcctcga ccaatcgccg ggcaagcagg caacgctgac cgaatgggct
      421 tcggccgtaa cgatcgcgga ctttcgggtc ttcacgcgtg atgtcgaaag cgctctgagc
      481 ctgacgagac gccatcaagt cgtacccttg gtcataatat tcgtcgagag ccagggccgg
      541 gtcgcccgca gccttgtcgg cgtatcgacg gaagtggtca agtcgatcgc ggagatcgcg
      601 tcggccatcc acccgattgt cggcgaggcc gcgcggtggg gcgacgtcgc ggacgcggaa
      661 gctcgccgcg ttcgggtcat caggcacgac gaacggggcg taccttgccc ccaggaagtt
      721 tggcccgccc gatcgcgtca tgctcggaat ggaaaagtac ggcggcagac caccggtggt
      781 cccgcgctcg tgggccgtga ccgcgcccat gctcgggtgg aagctcacga aagcaccgca
      841 gccgacgggg atccgcgtcg gcgcaccagt catcatgtaa tgattgccag ccccgtgatt
      901 cccctggtcg tggcggatcg accgaacgat cgcgatcttg tcggcgattc cggcgagccg
      961 cttcatcggc tcggagaact ggattccggg gaccttggtc gctattgggc caaacttccc
     1021 gcggatctcg gccggagcgc cgggcttggg gtcgaaggtc tcgtagtggc tcgggccgcc
     1081 gtccatccag atcaagatgc agctcgtcgg ccttatgtta gggatcgcgg cctgagcacg
     1141 gattcgtagc gcgtcgatca gcccaccacc gatcagggcg ctcaggccga ggcggaggca
     1201 gtcgcggcgg gttgtcgatt cgcagttctt gaagattgcc atcggtctcg cctttgtcac
     1261 tgaggtgcag cgggcggctt ggccggtctg cggtgcggat cagtccttga acacgaattc
     1321 gggggagttc agcagagccc aaagaaggtc ttcaaccgcg ccgcgacgat cgctttttgg
     1381 atccgcgaag agtgccaggg cgacgctcgc ctcttccctg gtcgggcgac ggctgtaggt
     1441 gagaagatac agttcctcga cgatggcgtg tggggccttg tcactcttgg ccagccgtgc
     1501 gactcgccct cggtcagcgg acagtttcgt gtgaactccg gcagaattca tgagatgaag
     1561 agcctgaaca accgccgtgt cgctcgtccg ttcacagggg ggatcttggt tcggatcagg
     1621 ccggccgaac gtatcgagaa agagcgaagg gacgcggttg gtccagatct ccagggcgcg
     1681 gctgcgagga gg
//
LOCUS       DATAQL010000170         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_171126, whole genome shotgun sequence.
ACCESSION   DATAQL010000170 DATAQL010000000
VERSION     DATAQL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171126"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..4054
                     /locus_tag="VHS97_02790"
     CDS             86..4054
                     /locus_tag="VHS97_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3447149.1"
                     /translation="MPDSNADCQLLFGVLAIQLRILSVASVSRCLSRWSASRESSLRC
                     ILIDQGVLDESAAALVDKAVEHHVRVANADVRGALEAFAGSDVLETLRGVLDQTMRFP
                     GPKEPTVARHDGLRFPVTAPANGNGRPKVQEPLTEADGEKFDDSDRTITNAGSSGRGA
                     GFQILRPLARGGLGEVFVARDGSLNREVALKLIQESQANDPQSKARFLVEAEITGGLE
                     HPGIVPVYALGESADGRPFYAMRLVRGETLKERIRKFHEGGSIRRQSLEFRQLLNHFV
                     RVCDVVAYAHDRGVVHRDLKPSNVMLGKFGETLVVDWGLAKPIDRTGETPAALQDELT
                     LRPASGSSVQATLHGSALGTPQYMSPEQALGQLDRIGPASDVYSLGATLYCILTGRPP
                     LASLGDVGEVLRRVALGDIPSARDSKIGIPDTLDAICKKAMAVRPEDRYQSAFALAGD
                     IESWLADEPPLGVRESLARRIGRWERRHRTFIRVSGLALIVVALVAIAAALGVNAARR
                     RAEERRRQAVTLGDIAETRKQDADRQRDALRRLTNRLTLDRGLGLLHDDDRREGLLWL
                     ARSLIGMTGQADPLEPATRINMAAWSSSLHRLKDCLDHKGPVRAVAWSPTGQSVATAS
                     DDGTARLWDPHSGAPLSPPLAHSGPIVALAYSPDGTTLATASEDQTARLWNAAAGLPR
                     GEPMRHSGPVTSLAFTSDGTTLYTGSADGMVRSWNSATGQPRRQTLEHGMPVRSIAIA
                     AQGRALASIDVHGSGILWDLASGNRQPIAAGSSSPVRSMAFSADGSKLACGCDDGLLR
                     LVDTKNGMILATTSRSLHSGPIITVAFRPDGNAVATGSYDTSCRLWSVPDLRPLGRPM
                     EQRGHVWNIAFSPDGSLLAAAYDDNKAQVWNLARYERHGDALPHTQSVRAVVFSGDGR
                     SILTACDEGSARIWQLGNAPSIGQSMDHATEVRALAARPDGKVIATADADGIIRLWDA
                     FTTRLIAKQHGHAPATTFEIAFNPAGTVLVTGSRDGLLKLWNGATLEPIATPIRMSSW
                     VRRVAISPDGTTLAAGDHLGQLGFWDARTGARLAPFAAVLKTVTSLAFNPDGTRLAVC
                     TSEGEGRIWDMTRFVPVGEPMRHKGSIQTAVYSPDGTRLVTGSYDKTARIWDARTMKP
                     LGSPLAHRAYVWSVCFSPDGERILTGSFDGTAQIWDGHTGHPRGEPMRHSDMIYGALF
                     SSDASMVLTFGRSRKAWLWDAASSRPVGEPLLHESEILHAAFLSDRSVVATASRDGTV
                     RLWSVPSAIAGASDRLAEEFTVLTGMKLGTDDVVRVLDFPDWKRKRDAFNDNTPTAQH
                     EK"
     gene            4199..>4803
                     /locus_tag="VHS97_02795"
     CDS             4199..>4803
                     /locus_tag="VHS97_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3447150.1"
                     /translation="MAHDETDRSDRRSFLQAGALATASAMTLAPAAAAAQDAAAKTTA
                     LPKRTLGRTGIEMTMLEMGTGALREKGVLDRLLKLAYASGVRTFDTAKMYGTEPGFKK
                     WFEQSPEVRKQIVLVTKDNPRTPKDMLGMLDKRLEALGTDYVDLFFIHGLGDEHKLSD
                     ALNFVTSQEFKETADAIRKSGKAKFVGFSSHHKDRAALIQAA"
BASE COUNT          878 a         1520 c         1538 g          867 t
ORIGIN      
        1 gagttacgcc tgccttcggc tctttgccgt ctgaaatctc tccgccgatt cattgaacca
       61 tcacaaccaa gctcgcagag gcgacatgcc tgacagcaac gccgattgcc aattactttt
      121 cggcgtgttg gccatccagc tgaggatatt gtcggtcgcg agcgtttctc ggtgtctgtc
      181 gagatggtcc gcgagccggg agtcatcgct caggtgcatc ctgatcgacc agggtgtgct
      241 cgacgagtcg gccgctgctc ttgttgataa ggcggtggag catcatgtcc gcgtggccaa
      301 cgccgacgtc aggggcgcgc tggaagcgtt cgccggcagc gacgtgctcg aaaccctgcg
      361 gggcgtgctc gatcagacca tgagatttcc cggccccaag gaaccgacgg ttgcgaggca
      421 cgacggactt cggttcccgg tgaccgcccc cgccaatggc aacgggcggc cgaaggtgca
      481 agagccgctg accgaggccg acggcgaaaa gtttgatgac tccgaccgta cgatcaccaa
      541 cgctggcagc tcggggcgag gcgctggttt tcagatcttg aggccgctgg ctcgaggggg
      601 cctgggtgag gttttcgtgg cccgcgacgg cagcttgaat cgcgaggttg cactcaagct
      661 aatccaggaa tcgcaggcga acgatcccca gagcaaggcc cgtttcttgg tcgaggcgga
      721 gattaccggc ggactcgagc atcccgggat cgtaccggta tacgcgctag gtgaaagcgc
      781 tgacgggcgc ccgttctacg ccatgcggct ggtacgcggc gagacgctta aagagaggat
      841 ccgcaaattt cacgaggggg gctcgatccg ccgccagtcg ctcgaatttc gccagttgct
      901 caatcatttc gtgcgggtgt gcgatgtcgt cgcctatgcc catgacagag gtgtggtcca
      961 tcgcgacttg aagccgtcca acgtgatgct gggcaagttc ggcgagacct tggtggtcga
     1021 ctggggtttg gccaagccga tcgatcggac cggtgagacg cccgcggcgc tgcaggatga
     1081 gctgactctc cggcccgctt ccggcagcag cgtgcaggcc accttgcacg gctcggctct
     1141 aggaacgccg caatacatga gcccggagca ggcactgggg cagctcgacc ggatcgggcc
     1201 tgcgagcgat gtgtacagcc tgggagcgac gctttactgt attctgacgg ggcgtcctcc
     1261 gctggcctcg ctgggcgatg tcggtgaggt attgcggcgc gtcgcacttg gcgacatccc
     1321 gtcagcgcgc gactcgaaaa tcggtatacc cgatacgctg gatgcgatct gcaaaaaggc
     1381 gatggcagtg cgtccggaag atcggtatca gtcggccttt gcgctggctg gcgacatcga
     1441 gagctggctg gcggacgagc ccccgctcgg agttcgcgag tcgctggccc gacggatcgg
     1501 ccgctgggaa cgtcgtcacc gcacattcat tcgcgtcagt gggctggcct tgatcgtcgt
     1561 cgctctggtg gccatcgccg ctgccttggg tgtcaatgcc gcccgccggc gcgccgagga
     1621 gcgccgccgc caggcagtca ctctgggaga catcgccgag acgcgtaaac aggatgccga
     1681 tcgccagcgc gacgcgctac ggcggctcac gaaccgtctg acgctcgacc gcggcctggg
     1741 actgcttcat gacgatgatc gccgggaggg gctgctctgg ctggctcgta gtttgatcgg
     1801 catgaccggc caagccgacc ccctcgagcc cgcgacacgc attaatatgg cggcctggag
     1861 tagctcgctg caccggttga aagattgcct ggaccacaag gggcctgtgc gcgcagtcgc
     1921 ctggtccccg acgggtcaat ccgtcgccac ggccagcgac gatggcaccg cccggctttg
     1981 ggaccctcat tcgggcgctc cactgagccc acccctggct cactctggcc cgatcgtggc
     2041 actggcctat tctccggacg gcacgacact ggccaccgcg agcgaagacc agaccgctcg
     2101 actctggaac gcagccgcag gtttaccgcg cggagagccc atgcgccatt ccggaccagt
     2161 gacctctctg gccttcactt cggacggcac gaccctctat acagggagcg ctgatgggat
     2221 ggtccgatcg tggaacagcg cgacgggcca accgcgcagg cagaccctcg agcacggcat
     2281 gcccgtacgg tcgatcgcga tcgcggccca gggacgggcg ctcgcgagca tcgacgtcca
     2341 tgggtccggg attctctggg acctcgccag tggcaaccgg cagccgatcg ccgcgggctc
     2401 atccagtccc gtccgctcga tggcgtttag cgccgatggg tccaagctgg cttgtgggtg
     2461 cgacgacggg ctgctacggc tcgtcgatac gaagaacggg atgatcctgg caacgacgtc
     2521 gagatctctg catagcggac cgatcatcac cgttgccttc cggcccgatg ggaacgcggt
     2581 tgcgaccggc agctacgata cttcgtgccg tctctggagc gtgcccgacc tgcgtccgct
     2641 cggccgcccg atggagcagc gcggacatgt ttggaatatt gcctttagcc ctgacggctc
     2701 gctcctggca gcggcctacg acgacaacaa ggcccaggtg tggaatcttg cccggtatga
     2761 gcgtcatggc gatgctctgc cgcacaccca gtccgtccgc gcagtcgtct tcagcggcga
     2821 cggccggtcg atcctcacag cttgcgacga aggatctgcc cgaatctggc aactcggcaa
     2881 cgccccaagc atcggccagt cgatggatca cgcaacggaa gttcgcgctt tggccgcccg
     2941 ccccgacggc aaggtgatcg cgaccgcgga cgccgacggc atcattcggc tctgggatgc
     3001 cttcaccacg cgcctgatcg caaaacaaca cggccatgct cctgcgacca cctttgagat
     3061 cgccttcaat cccgcgggga ccgtgcttgt gacgggctct cgtgacggtt tattgaaact
     3121 ctggaacggt gcgactctgg agccgatcgc aacgccgatc cgcatgagca gctgggtgcg
     3181 ccgcgtcgca atcagtccgg acggtacgac gcttgccgcc ggcgaccacc tcggccagct
     3241 cggcttctgg gacgcgcgca ccggcgcacg cctggcaccg tttgctgcgg tcttgaagac
     3301 ggtgacgtcg ctcgcgttca accccgatgg cacacgcctg gcggtctgca cttctgaggg
     3361 cgaaggccgc atctgggata tgacgcgctt tgtgcccgtg ggagagccga tgcggcacaa
     3421 gggctcgatt cagaccgccg tctacagccc cgacggaacg agactcgtca ccggcagcta
     3481 cgacaagacg gcccgaatct gggacgcgcg gacaatgaaa ccgctcggca gtccgctagc
     3541 gcatcgtgcg tatgtgtgga gcgtttgctt tagtcctgac ggcgagcgaa tccttaccgg
     3601 cagctttgac gggaccgcgc agatctggga cgggcacacc ggtcacccgc gcggcgaacc
     3661 gatgagacac tccgatatga tctacggagc cctcttcagc agcgacgcgt cgatggtact
     3721 cacgttcggc cgttctcgaa aggcatggct ctgggatgcg gccagctccc ggcccgtggg
     3781 ggagcctctg ctccacgaaa gcgagatctt gcacgcggcc ttcctttccg atcgctcggt
     3841 cgtcgccacg gcatcgcgtg acggcacggt ccgcctctgg tccgtcccat cggcgattgc
     3901 gggcgcctcg gatcgcttgg ccgaggaatt caccgtcctg acaggcatga agctgggcac
     3961 tgacgacgtt gtccgcgtgc tcgatttccc ggactggaaa cgaaagcgcg acgccttcaa
     4021 tgacaatacc cctaccgcgc agcatgagaa gtgacaatcg atggtcgcag acttcctgat
     4081 tgaagagcag tctggacgag ggtaaagtac tatgttattc gtactggcga caagcctgat
     4141 gcctgatgat caccagagag accggcgcct tatcaccatt cacgaggagt cctgactgat
     4201 ggcccacgat gaaacggacc gttctgaccg tcgctcgttc ctccaggccg gagcgctggc
     4261 gacggcctcg gcgatgactc tggcgccggc cgcggcggca gcccaggatg ccgccgccaa
     4321 gacgacagcg ctgccaaagc gaacgctggg tagaaccggt atcgagatga cgatgctcga
     4381 gatgggcacc ggtgccttgc gcgagaaagg agttttggac cgcctgctca agcttgccta
     4441 cgccagcggc gtgcgcacat tcgacaccgc caaaatgtac ggcaccgagc ctggcttcaa
     4501 gaaatggttc gagcaatcgc ccgaggttcg caagcagatc gtgctggtca ccaaagacaa
     4561 tccgcgcact cccaaggaca tgctcgggat gctcgacaag cggcttgagg cgcttggtac
     4621 agattacgtc gacctgttct tcattcacgg tctgggtgat gaacataaat tgagcgacgc
     4681 gctcaacttt gtgacatcgc aggaattcaa agagacggcc gacgcgatcc gcaagtccgg
     4741 caaggcaaag ttcgtcggtt tctcgagcca tcataaggat cgcgccgcct tgatccaggc
     4801 ggc
//
LOCUS       DATAQL010000171         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_781099, whole genome shotgun sequence.
ACCESSION   DATAQL010000171 DATAQL010000000
VERSION     DATAQL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781099"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            767..934
                     /locus_tag="VHS97_02800"
                     /pseudo
     CDS             767..934
                     /locus_tag="VHS97_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1098..1883)
                     /locus_tag="VHS97_02805"
     CDS             complement(1098..1883)
                     /locus_tag="VHS97_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447151.1"
                     /translation="MVWSVLVSLFRSRVSLEAEILILRHQLNIQRRYLPKRLTFSAMD
                     RLIFAGLYRLMPSTLNALTLVKPETVVGWHRAGFRSYWRWKSRPRSGRPTVPAEIRRL
                     IREMGIANPLSGAPRIHSELLKLGIEIGQTSVAKYMARRRGPPSQSWKTFLRNHADGI
                     AAMDLFVVPTISFRLLYGLLIMGHGRRQVLWFGVTDHPTAEWIANQLTQGLWLGANPS
                     LPDPGSRRSLWRDIPPPDSIDRHSRSPDLRSFTARSAGNALIM"
BASE COUNT          414 a          604 c          572 g          395 t
ORIGIN      
        1 gcatctcgtt ctccctaagc tcaccaatct aggcgcggct ccgcgattga cttaacgtcg
       61 gatcgaccgt gcttcaatct tctcaatcgt ctaataggtc gaacgctgtt tcctgttctc
      121 ggggcttctg ggagtgagcg cttcggccgc agaccccgtc ggtcgataga atcgttgtct
      181 cggaccaatt cgagcagctc gcctcgatcg gatgtccaag gtcaatcgcg ccgttccgat
      241 cagacagtta cttccgtacc tggcccttct cggtcctgag gcgatgccaa cggcgcggct
      301 gcagtttaag ctaaagcaga ctttccgacc cttcttcatg acgacgagcc ggccgccttg
      361 gacctattcg gcacgagccg cccatccatc gcccgcgcgt ggatgtcagg tcagccagca
      421 cgctaggccc gctgcgatcg gcgcacggac gtgctcggct gaggcgcgtg gtgacagact
      481 gaggcgttgg gactatgcta gccgcgcaac gttcgtgcgg agcacttatg agtaacgagc
      541 ggaatagcca ccgtagcacc atccagcttt tcgcggttgc ggtggcaatc gcgatactag
      601 cggctttcgt cacgactctt gagcgagtcg gcacaaggac ggctgacaac caaaccgcgc
      661 cgggtacaac gggccttgcc aagccacatg cacctttgga cagagcaccc ggtcagccgg
      721 tgctacgcaa ctgagcgcac acgcaggtct acggctggcg gcttgccatg gccgcggctc
      781 catcctcatc accagccaac tccccgtggc aacttggcac gaggttattg gcgagccgac
      841 gctcggcgac gccatcttgg accggtttgt ccacaacgcc taccggctcg aactcgatgg
      901 tgcgcctgcc gtaaatcaaa tccggccgta ttgatggtgc agtccgccca gcaacggacg
      961 gctgataatg cgtccgactg cctcagcggc gcgcgagttc ggcgcatcct tgttcaatga
     1021 caagtgagtt cgggtgtcgt tgtaatagtt catgtacgaa agtaacacgt ggcgcagatg
     1081 ccgttcgcca aacactacta catgatcaat gcattccctg cggatcgagc cgtgaacgag
     1141 cggaggtcgg gcgatctcga atgccgatcg atcgaatccg gcggaggaat atctctccat
     1201 aagctccgtc tcgatcccgg atcaggaagc gagggatttg ctcccagcca caggccctga
     1261 gtgagctggt tggcgatcca ttcggccgtc ggatggtctg tgacgccaaa ccataaaacc
     1321 tgtcgtcgac catgccccat gatcaaaagg ccgtagagca gcctaaatga gatcgtgggc
     1381 acgacgaaca gatccatcgc cgcgatgccg tcggcatgat tgcggaggaa tgtcttccag
     1441 ctttgggacg gcgggcccct tcgcctcgcc atatacttgg cgacgctggt ctggccgatc
     1501 tcgatgccga gcttgagcaa ctcgctatgg atccgcggcg ctcccgacag cggattggcg
     1561 atgcccatct cgcggatcag ccgccgtatt tcggccggaa ctgtcggtcg gccggaacga
     1621 ggtcgtgact tccagcgcca gtacgatctg aacccggcac gatgccaacc gacgacagtc
     1681 tccggcttca caagcgtcag cgcattgagg gtacttggca tcaaacggta cagtccagca
     1741 aagatcaggc gatccatggc actgaaggtc agtcttttcg gcagataccg gcgctggatg
     1801 ttaagttgat gacgaaggat caggatctca gcttccaacg agacccgcga ccggaacaat
     1861 gagaccagca ccgaccagac catgctgcaa gcttctctca taaggcagaa gcattgcgcg
     1921 attcggcctt acgtgccagc cggattagat tttcgacgac agggtcagct acagaaagca
     1981 agggc
//
LOCUS       DATAQL010000172         4161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3530778, whole genome shotgun sequence.
ACCESSION   DATAQL010000172 DATAQL010000000
VERSION     DATAQL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3530778"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VHS97_02810"
     CDS             complement(<1..230)
                     /locus_tag="VHS97_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447152.1"
                     /translation="MRRWRFIRFMTLGLVLLALPARLPAQDTAKTDVAPAQAPGVTAQ
                     PDATEAASTTEKAFHYFGFTTDPAYGIWEKISL"
     gene            complement(629..1672)
                     /locus_tag="VHS97_02815"
     CDS             complement(629..1672)
                     /locus_tag="VHS97_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3447153.1"
                     /translation="MPKSNALISLGKSPLTDLRSLGSSGLFHLLVVLLAWVTVLNVAL
                     PLTANRPKALYAEVDPVDNRADVPPSPGQGGGNPGDIGGMSDIPLVTPSDGKSPQGAT
                     RDPVAETLLAEILPNPQPKPSDTIQRALPGPQTAGQGLIPGSGSGGGGGAGGGSGGGA
                     GRGIGPGTQFFGARDHAHSFAYVIDCSGSMAARNSLEVAKREMLASISQLPPDAQFAV
                     VFYNLQARMLTDPLGKKGLMAATAPNKARVQTQLATIAPDGGTDHMTALRAALALKPE
                     VIFFLTDADLMSNNDVNEILAEVGPTRIQAVEFGRGTELGQRTPLGRLATTTGGSYLY
                     IDVSHFPRSSSGF"
     gene            1937..2635
                     /locus_tag="VHS97_02820"
     CDS             1937..2635
                     /locus_tag="VHS97_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447154.1"
                     /translation="MKRLCVVTAALVGFFFPIAPGDDQEIKLSDCPQAVRKTFQAEAK
                     GAKIETVTREREADAEALYWAEVDLGGKKYEIGILEDGTLTEMNLAVSDEKISLERCP
                     AAVQETFSGEALGEKVVEIGRDVKYGVTIYQTVVNHKGKSYTVIVAEDGTLMEKVLVI
                     NDEEVKLSDCPAVVRSSLHLHANGGEIGDITRSTGIVRPTFEAEVKIKDKIYLIEVDE
                     TGLLVSKSLEAGED"
     gene            complement(2711..3760)
                     /locus_tag="VHS97_02825"
     CDS             complement(2711..3760)
                     /locus_tag="VHS97_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447155.1"
                     /translation="MITSSRWNRGTVPWALSIGLLIVPQAVAHGGGHGGGHGGGHGGG
                     HAVQAPHFSVRQPAHNMPARMPRAAAPRNINNNSGATHPHGSTSGARALTNQTHAQSN
                     ANHSQALASGTRTGHNSAATTTTTSSTHARNNAATIASSTHTRNNVAPTSGTGRPTAL
                     GTMPTLSAGYSLVPNAYTYGYGRGARSYRAYSYGSGYRNRSYGRGYGYGRSQGLNRGV
                     LAGLVSTRASLARVPHDYQGHRGRAIHAITMAIRQLSHRSMSYNGMGNSSGMNNGGAV
                     GMRQGAGGAGAGALQPQRMSQAQSDNRMGQALRRLQGINMQLASQGSYTNGHARARGH
                     VQQAIHELNFALSIR"
     gene            3759..4127
                     /locus_tag="VHS97_02830"
     CDS             3759..4127
                     /locus_tag="VHS97_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447156.1"
                     /translation="MATQLLTVHEWTTSAVTGQRRVHAGLTSIDSSLEHHSYVEFNPR
                     AMRKYRGVRGRQGSLSGKRAWFNFSIFAGEKSWVAGVELATVSEPPARLPRIWGRRPS
                     GVDPSHPLRCNQLLNLAGKR"
BASE COUNT          854 a         1189 c         1303 g          815 t
ORIGIN      
        1 agcgagattt tctcccagat gccgtaagcc gggtcggtgg tgaaaccaaa gtaatggaac
       61 gctttttcgg ttgtggaagc cgcttcggta gcgtctggtt gagccgtgac gcccggcgcc
      121 tgcgccggag caacatccgt cttggccgtg tcttgcgccg gcagacgagc gggcaatgcc
      181 aaaagcacca gtccgagggt catgaaccga atgaatcgcc aacgtcgcat gatccttatc
      241 tccaggttca agggttgtca cattctcggc gaataagggt cgtcagtcgc ttgacattgc
      301 accgcgactg ttgttgcgcg tgcgctgcaa gcactgtcgc atcagtcaaa gtggtaatcg
      361 tcggtgagcg aacagatcgg actaagatcg gtgccgccgc aaggctcagc tcatacattt
      421 ccgccgacga tcgcacgagc gccggcactg gtcgcaccca cagattttca ccacaaactc
      481 acgggggaca tacagtagca cgaaatggaa aacacattca accgggggta cccccttgtt
      541 ggaggtcacc gagtggattt cgcgcacgaa cgcgcgtcat ccagagaaaa gtcccccaaa
      601 aaaagcgggc cgaacggcga cgcccgcttt agaaaccgct actcgatctg ggaaagtggc
      661 tcacatcgat ataaagatag gagccgccgg ttgtggttgc caatcgaccc agcggagttc
      721 gctgtccgag ctcggttccg cgcccgaatt cgaccgcctg aattctcgtc ggcccaacct
      781 cggccaatat ttcgttgaca tcattgttcg acatcagatc cgcgtcagtc aggaagaaaa
      841 tgacctcggg tttgagcgcc agcgccgcgc gtaaggctgt catatggtcg gtgccgccat
      901 cgggggcgat cgttgcaagc tgcgtttgca cccgcgcttt gttgggtgcc gtagcggcca
      961 tcaatccttt ttttcccagg ggatctgtga gcatgcgcgc ctgaaggttg tagaaaacta
     1021 cggcaaactg ggcgtctggc ggaagctgac taatgctggc aagcatttca cgcttggcaa
     1081 cctccagcga gtttcgggcg gccatgctac cggagcaatc gatcacgtag gcgaaggaat
     1141 gagcgtgatc gcgtgcgccg aaaaactgag tgcctggtcc aatcccgcga ccggcgccac
     1201 cgccagaacc cccacccgcg ccaccgcctc cgccggagcc cgagcccgga atgagcccct
     1261 ggccggccgt ctgagggcca ggtagggccc gctgaatggt atcgctcggc ttgggctggg
     1321 gattgggtaa gatctcggcc aagagcgtct cggcgaccgg gtcacgggtc gccccctgag
     1381 ggcttttgcc atcggacggc gtcaccaagg gtatatcgct cattcctcct atatcacccg
     1441 gattgccacc tccttggccc ggtgaagggg gaacatccgc tcgattgtca accggatcga
     1501 cctccgcgta gagcgccttg gggcggttcg ccgtcagcgg caaggcgacg ttcaaaacag
     1561 tcacccatgc cagcaaaacc acgagcaagt gaaacaaccc cgaagacccg agcgaacgga
     1621 ggtctgtgag gggactttta cccaatgaaa ttagcgcgtt tgatttgggc acgagaggat
     1681 cactccgcta tagtcacagc tactggaaat tgtcacatcc ctggatggcg ggtcaagccc
     1741 aggcaatgag ccgatggcgt ggaaatctca gcgcgggcct cggccatgtt tcgaatgaga
     1801 cgcgatgtag ggcggtgact gtacgattct tcagagccta ttgaccgctc cgcatcgcca
     1861 tcggacactc aagatatgat cttgtgcgag ttgggttgac gtcgtcgcgc tcttatgaag
     1921 aggggaaagg gtcgtcatga aacgtctgtg tgtcgtgaca gccgcgctcg tcgggttctt
     1981 cttcccgata gcgccgggcg acgatcaaga gatcaagttg tccgactgcc cccaagcggt
     2041 acgcaagacg tttcaggcgg aggcgaaggg agcgaagatc gagaccgtca ccagggagag
     2101 agaagcagac gccgaggcgc tctactgggc tgaagtagac ctgggcggca agaagtacga
     2161 gatcggcatc ctcgaggacg gcacgctcac cgaaatgaac ctggccgtca gcgatgagaa
     2221 aatctcctta gagcgttgcc cggccgctgt ccaggagacg ttcagtggcg aggcacttgg
     2281 cgagaaagtc gtcgagatcg gcagagatgt gaagtacggc gtgacgatct accagaccgt
     2341 cgtgaatcac aagggcaagt cctacacggt cattgtcgcc gaggacggaa ccttgatgga
     2401 aaaggtcctg gtgatcaatg acgaggaggt caagctgtct gactgcccgg ccgtcgtgcg
     2461 gtcttcattg cacttgcacg cgaacggcgg cgaaatcgga gatatcactc gttcgaccgg
     2521 gattgtcagg cccacgttcg aggccgaggt caagatcaag gacaaaattt atctgatcga
     2581 ggtcgacgaa acgggattgt tggtctcaaa gtcgctcgag gccggcgaag attgaacggc
     2641 cgcgacgaga tgccgagcat tggtggcggg agtaagtaat ttgcccgtgt tgctgatcgc
     2701 cgctgctcga tcagcggatc gagagggcaa aattcagctc gtggatcgct tgctgcacat
     2761 gtccgcgtgc ccgcgcgtgc ccgttggtat acgacccctg gctggcgagt tgcatgttga
     2821 ttccctgcaa tcgccgcaga gcctgaccca tccggttgtc ggattgcgcc tgcgacatcc
     2881 gttgcggttg gagcgctccc gctcccgcgc cgccggcacc ctgtcgcata ccgaccgccc
     2941 ctccgttgtt catgccggac gaattgccca tgccattgta gctcatcgat cggtgtgaaa
     3001 gctgccgtat cgccattgtg atcgcgtgta tagcccggcc gcggtgaccc tggtaatcgt
     3061 gcggaacacg tgcgagactc gcacgtgttg aaactaaccc agcgagcact ccgcgattga
     3121 gtccttggga cctcccatag ccatagcccc ggccgtaaga gcggttacga tagccactac
     3181 cgtagctata ggcccggtag gagcgagcac cgcgaccgta gccatacgtg taggcattcg
     3241 gtacgaggct atagcccgcg ctgagtgttg gcatggtccc cagcgcggtg ggacggccag
     3301 ttccgctggt cggcgcgacg ttattccggg tgtgggtgct actggcaatg gtcgcggcgt
     3361 tattgcgagc gtgggtgctg ctggtggtgg tggtggtggc cgcggagttg tgaccggtgc
     3421 gggtgccact cgcaagtgct tgcgaatgat tggcgttgga ttgagcatgc gtctgatttg
     3481 ttagggcacg cgcgccactg gtcgacccgt gcggatgagt ggcaccgcta ttattgttaa
     3541 tattgcgcgg cgccgccgcg cgaggcattc gggctggcat gttgtgcgcc ggctggcgca
     3601 cggagaagtg gggcgcttga acggcatggc cgcccccatg tccaccgcca tgaccacctc
     3661 catgaccgcc gccatgcgca actgcctgcg gcactatcag tagcccgatc gaaagcgccc
     3721 aaggaacagt gccgcgattc cagcgagacg acgtgatcat ggcaacacag ctcctgacgg
     3781 tgcatgaatg gacaacaagc gccgtcaccg gccaacgacg tgttcacgcg ggcttgacgt
     3841 ctatcgactc ctcgctcgag catcactcgt acgtagaatt caacccccgg gcaatgcgga
     3901 agtatcgcgg cgtccgtggg aggcaggggt cattgtcagg aaaacgggcg tggttcaact
     3961 ttagcatctt tgcaggtgaa aagagctggg tggctggggt cgagctggcg acagtcagcg
     4021 agcccccggc gcggctgcct cggatctggg ggcgtcgccc ttcgggcgtc gatcccagcc
     4081 acccactgag gtgtaaccag cttttgaacc ttgccggaaa acggtgatac gcttgacctc
     4141 aagaccggcc gttttcagga a
//
LOCUS       DATAQL010000173         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5057249, whole genome shotgun sequence.
ACCESSION   DATAQL010000173 DATAQL010000000
VERSION     DATAQL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5057249"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..499)
                     /locus_tag="VHS97_02835"
     CDS             complement(113..499)
                     /locus_tag="VHS97_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447157.1"
                     /translation="MFLLWGLLCLLGGTNTSQAAGCHMQDRLVLRSTLSWEKQLAVDL
                     NSEPLVQLPPVLAKPPCQGEVPHVVDSDFAWPSAAFVQPLRFGPPGLSDFLSFQFPSE
                     PIRPPLLRLDRPPRTTVTCTAVERLA"
     gene            1331..3079
                     /locus_tag="VHS97_02840"
     CDS             1331..3079
                     /locus_tag="VHS97_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3447158.1"
                     /translation="MLQLTLMLFFAPLFTAAAVSYEKDRRTLSLLLMTDLSDLEIVLG
                     KLIAGLLNILSILAASIGFLSICAFFGGISFGQVVNLFAVTAASGVAGGALGLLIALW
                     RDRTFQSISLTILMVVFSVAGVEAFAIAYPTLEFMHVPLVEVLNPYRAMLAVLYPQTD
                     QLTGVVRASSVVYIAARLTFAAALVAFGTFMLRKWNPGRNEPRELREEGEVEAVETLV
                     EIDEETAEEAVGVSSAATAAIGQSGVAVASRPHADVAVASTSARGSDALGEPGPTTGL
                     HVPRRTHRRIGAAARAYRHPWANPILWRELKTRAYGAKPLIIKGCYILLFALGMGLFF
                     HLGQEMENPLRSTLGLIPIGLAILSLVLVNAQGVTSITSERDTGALDLLLVSELSPKE
                     FIYGKLYGVLYNSKEMIALPVLTAIAMFVLGIFSGENLVLFVIDFLLLCHFAAMLGLH
                     AAITYTSSRTAVANSLATIFFLMVGILLCAFLIALNNQEFGRQLLSFMIFIGAGSVAL
                     FGSLGAKNPSHAIALVALLTPFWTFYCIISLVNADIMAAFLVSAGIYGFAILAMLVPA
                     VGDFDIALGRTSAIQG"
     gene            3115..>4306
                     /locus_tag="VHS97_02845"
     CDS             3115..>4306
                     /locus_tag="VHS97_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3447159.1"
                     /translation="MLTRRLHKSASIVVFACAAFAFAPFPSWLTAKAQDDGLANELPR
                     IKPLEPAAALGSFRLQPGFQLEPVATEPLVTNPVSACYDADGRLYVVEMRGYPYPEKS
                     PSGGVSRLEDRDGDGQFDARKVFLGGLSWPTGIVPYDDGVFIAAAPDILYAKDTNGDG
                     VADDKKVMFSGFGTENVQGLLNGLLWGPDGWIYGVTSSNGGTIHNRSRPDVKPVAVRG
                     RDFRFKADGSAFEAVSGGGQFGHSFDDWGHRFTCNNSNHVRQIVLPSRYLERNPALVP
                     PSVILDIAAEGPAAPVFRISPAEPWRVVRTRQRAADPVLSRRLPPTELFAAGLFTSAT
                     GVTVYRGSSYPPNYRGNVFVGDVGGNLVHRKILDAEGQTYRAWRADRNTEFLASTDNW
                     FRPVN"
BASE COUNT          781 a         1318 c         1277 g          930 t
ORIGIN      
        1 tctgtaaaag cgcgatcggt ttccacgcgc gcgaatccac gcaagaccct cccccgctgt
       61 cccagtccaa tgacgtaaat ggaatggaaa aagctcaagg cgcaatcgcc gttcaagcca
      121 gccgttcgac cgcggtacac gttacggtgg tccgaggggg acgatcgagg cgcaacagcg
      181 gcggccgaat gggctctgag gggaattgaa aggagaggaa gtccgataag cccggagggc
      241 cgaagcggag cggctggacg aacgcagcac taggccaggc aaaatcggaa tcgacgacgt
      301 ggggaacctc gccctggcac ggcggcttgg caagtactgg aggaagttga acgagtggtt
      361 cagagttcaa atcaaccgcc aactgctttt cccacgacag cgtcgaccgc agaacaagcc
      421 gatcttgcat gtggcagccc gcggcctggc tggtattggt cccaccgagc aggcacaaga
      481 gtccccacag caagaacatc catatgcgac cagagtgtgc tggaaccgcc cgagacacgc
      541 cgagatttgc tccgaatgga ctgcgcacgt ttgtgaggaa aggctgaact cgatcgtaag
      601 aggccgcgtt gccgattgtc aatgggacca ggctatcgag gacgacccat cgaccatcct
      661 catttccttc gttgcttcgt tcgcatcgtc tgaagatcat ccccaaccca gtcttggctg
      721 agtgcgcgct tccgggcgtg catgccctcg agacggctcc ggtacattgc aactcggcca
      781 gcccacggac gccaataagc tgcggccgca actagccggc accacggcct accgagccgg
      841 ttcaccatgc cagcttcgag atccacgaga agacgctcgc cggcccgact tggagagaga
      901 attcggcatc tggtacactt gatctctggc aatttgttcg gaggctccga acaaactgca
      961 gtagtgcgaa cgtaagacat gccagggagc accgcgcgcg cgactcggct tcgagtgtgc
     1021 gtcctgccag cgcaagcgca acaagagccc ggcacggttc gaccgcggtt tttctaggaa
     1081 tggtacgtta tcctcatcct tcgtgccctc gccgacgtgg gaggtttgac ctttgtttgc
     1141 cggaccgatt attgcccgag aggttttaac ggcccctcgg ccattgcgtt actacctttg
     1201 gcgtgcttcc tttgcttgct ttctgttcat acttttgtgg acggcgtggc aatcgttcat
     1261 tggctgggag tatgtgcgag aattaggggt tctagctcgg tttggcggca tcctatactg
     1321 gatgttcgcc atgctccagt tgacgttgat gctctttttc gccccgttgt tcaccgcggc
     1381 cgcggtctca tacgagaaag accgccgcac gttgtccctt ctcttgatga cggatctgag
     1441 tgacttggag atcgtgctgg gcaaactgat cgccgggctg ctcaacatat tgtcgattct
     1501 ggcggccagc atcggattct tgtcgatttg tgccttcttc ggtggcatct ccttcggcca
     1561 ggtagtgaat ttgtttgccg tgacggcggc ctctggtgtg gccggcggag cgctcggctt
     1621 gttgatcgca ctttggcgtg accgcacgtt tcagtcgatc tctttgacga tcttgatggt
     1681 ggtcttttcg gtcgcgggcg tcgaagcctt tgcgatcgcc taccccacac tcgaattcat
     1741 gcatgtgcca cttgtcgaag tcctcaatcc ttatcgcgcc atgctagccg tcctctatcc
     1801 ccaaaccgat cagcttaccg gtgtcgtgcg ggcctcgagc gtcgtttata tcgcagcccg
     1861 gctcacgttc gcggcagcac tcgtggcttt tggaaccttc atgctccgca aatggaatcc
     1921 cggccggaat gagccgcgcg agctccgcga ggaaggcgaa gtcgaggcgg tcgagacgtt
     1981 agttgaaatt gacgaggaaa cagcggaaga agccgtggga gtgagtagtg cggcgacggc
     2041 tgcgatcggg cagtccggcg tcgcggtcgc cagccgtccc cacgcggatg tggcggtcgc
     2101 gtcgacttcc gcacgcggct cagatgccct cggtgagcca gggccgacca cgggcctcca
     2161 cgtcccccgc cgaacccacc gccggatcgg ggctgccgcg agagcctatc gccacccttg
     2221 ggccaacccg atcctctggc gcgagctcaa gacgcgcgct tacggggcca agccactgat
     2281 tatcaagggg tgctatatac tactattcgc tctgggcatg gggctctttt tccacctcgg
     2341 ccaggaaatg gagaaccctc ttcgctcgac cctggggctg atccccattg gtctggcgat
     2401 cctcagccta gtcctggtca atgcccaggg ggtcacatca atcacaagcg agcgcgacac
     2461 cggcgcgctc gatctgctcc tggtatccga gctgtccccc aaagagttca tatacggcaa
     2521 gctttacgga gtgctttata acagcaagga gatgatcgcg ctgcccgttt tgactgcgat
     2581 cgccatgttc gtactcggga tattctccgg cgaaaatctg gtgctgttcg tcatcgattt
     2641 cttgctcctc tgccatttcg cggcaatgct ggggctgcac gcggcgatca cctataccag
     2701 tagccgcacg gcggtggcca acagcctggc gacgatcttc tttctcatgg tcggcatctt
     2761 gctctgtgcg tttttgatcg cgctcaacaa ccaggagttc ggccgccaac ttctcagctt
     2821 catgatcttt atcggggccg gcagcgtggc gctatttgga tcccttggag ccaagaaccc
     2881 gtcccatgcg atcgctctcg tcgcactctt gaccccgttc tggacgtttt actgcatcat
     2941 cagcctggtc aatgccgaca tcatggccgc gtttttagtc agtgcgggca tttacggctt
     3001 tgccatactc gccatgctgg ttcctgccgt cggcgacttc gatatcgcct tgggccgcac
     3061 cagcgcgatc cagggttgac ccggcttttt tcccgctgat gttggaaaaa aatcgtgttg
     3121 acccgtcgcc tgcacaaatc cgcgtcgatt gtcgtattcg cctgcgccgc ttttgccttc
     3181 gcgccgtttc cgtcttggct aactgccaaa gctcaggacg acggcctggc caatgagctg
     3241 ccgcggatca agccgctgga acctgcggcg gcgcttggtt cgttccgcct gcagccaggc
     3301 tttcagctcg aaccggtagc gacagagccg ctggtgacca acccggtcag cgcctgctac
     3361 gacgccgatg gacggcttta tgttgtcgag atgcgggggt atccctatcc agaaaaatcg
     3421 ccctctggcg gtgtcagccg tctcgaagac cgcgacggcg acggccagtt tgacgcgcgg
     3481 aaggtcttct tgggcggtct ctcgtggccg accggaatcg ttccctacga cgacggcgtg
     3541 ttcattgcgg ccgctcccga cattctgtat gccaaggaca ccaacggcga cggcgtcgcg
     3601 gacgacaaga aggtgatgtt cagcggattt ggcacagaga acgtccaggg actcttgaat
     3661 ggtctgctct gggggccgga tggctggatt tacggggtaa ccagcagcaa cggcgggacg
     3721 atccacaacc gatcgcggcc cgacgtcaag ccagtggccg tgcgtggacg cgactttcgc
     3781 ttcaaggccg acggctctgc gttcgaggcc gtttcgggcg gaggccaatt cggccattcg
     3841 ttcgacgatt gggggcaccg cttcacctgc aacaacagta accacgtccg ccagatcgtt
     3901 ttgccctcgc gctacctcga gcggaatccc gccttggtcc cgccgtccgt gattctcgac
     3961 attgccgcgg agggaccggc cgcgccggtg tttcggatca gcccggcgga gccctggcgg
     4021 gttgtacgaa cccgccagcg cgcagccgat ccggtgctct cgcgtcgcct gccgccgacc
     4081 gaacttttcg ccgcgggcct cttcacctcg gcgaccggcg tcacggttta tcgcgggtcg
     4141 tcctatccgc caaattaccg cggcaatgtg ttcgtggggg acgtgggcgg aaatttggtc
     4201 catcgcaaga ttctcgacgc cgaaggacag acatatcgcg cctggcgtgc cgatcggaac
     4261 accgaattcc tggcctcgac cgacaactgg tttcggccgg tcaact
//
LOCUS       DATAQL010000174         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1393036, whole genome shotgun sequence.
ACCESSION   DATAQL010000174 DATAQL010000000
VERSION     DATAQL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393036"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VHS97_02850"
     CDS             <1..653
                     /locus_tag="VHS97_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX3447160.1"
                     /translation="ARILYRKGWIRRSFFLRALYHGLQHRFGRLDYGRLIAIGMNSIA
                     RIPVVELERIAYENFVEFVRPRLYEGVVEHLYGLRRQGTAIVLVSSSPGLVIQPLSIY
                     LSCTDTLTTPVVVERGRLVAVGSGPACYGEGKRYWAEEWARSRNVRMEDAVAYADNWS
                     DRALLERVGRAVVVHPRGRLLRLARAKNWDIVRPKRPSVAKSLGDKALSHERGPTT"
     gene            956..1633
                     /locus_tag="VHS97_02855"
     CDS             956..1633
                     /locus_tag="VHS97_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447161.1"
                     /translation="MNDKTPAVPGQDPPVYSESLLSRTKAGPAAVALVIGLAALTAQA
                     SETDLVRDIQRYCTVCWRNARLDPRLWDDCTQEVCCRLLGKARIGQLDLTQVMADDTP
                     ERRELVRAIDMVRKRVQRTKKYQPLDTFTTPVTDLDQRQRNRLELGEILEEARRAVLT
                     ARQDRIIELWMRGWSVPEIGAELKLPVTRVSDEKYKALRKLEHQLSNRREELEFGLPS
                     MALANLD"
     gene            complement(1683..2144)
                     /gene="bcp"
                     /locus_tag="VHS97_02860"
     CDS             complement(1683..2144)
                     /gene="bcp"
                     /locus_tag="VHS97_02860"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX3447162.1"
                     /translation="MVEEGKPAPDFTLADQGGKKVTLSKLKGSSVVLYFYPKDDTPGC
                     TKEACEFRDAFADYKKAGAIILGVSPDDSDSHAKFAKKFELPFTLLADNDKKVSEAYG
                     VWKEKSMYGKKYMGVERTTFVIDRQGKIAKIFPKVKVDGHSSAVLAAIKAG"
     gene            2352..>2580
                     /locus_tag="VHS97_02865"
     CDS             2352..>2580
                     /locus_tag="VHS97_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX3447163.1"
                     /translation="MGRSETFEHTADLGLRIFATDLPDLFRTAGEALFDVIVANRSDV
                     RVVETELVSLTAESIADLLVQWLNELIFRCET"
BASE COUNT          506 a          728 c          796 g          550 t
ORIGIN      
        1 tcgcgcggat cctctatcgc aagggctgga tccgccgctc attctttttg agggcactct
       61 accacgggct ccagcatcga ttcggccggc tcgactatgg ccggctgatc gccatcggca
      121 tgaactccat cgcgcgcatc ccggtggtcg aactcgaacg gatcgcttac gagaatttcg
      181 tggaattcgt tcgaccccgt ttgtatgaag gggtcgtcga acacctctac ggcttgcgcc
      241 gtcaagggac ggcgatcgtg ctcgtatcgt cgtcgccggg actggtgatt cagcccctga
      301 gcatttattt gagctgtaca gatacgctga cgacgcccgt ggtggtcgag cgtggccgcc
      361 tggtcgcggt gggttcgggt ccggcttgtt atggcgaagg taagagatat tgggccgagg
      421 aatgggcgcg ctcccgcaac gtccggatgg aagacgcagt cgcgtatgcc gataactgga
      481 gcgatcgggc cctgctggaa cgggtcggcc gggcggtggt ggtacatccc cgaggcaggc
      541 tcctgcggtt ggctcgggca aagaattggg atatcgtgcg gccgaaacgg ccgtcagttg
      601 caaagtcctt gggagataaa gcgttgagtc acgaacgcgg cccaaccacg tgagccatgt
      661 tgcttgccag gatcgtgaaa aaaccgcctg ccgacgatcc cggcgaaccg atattcttgg
      721 cagaaccgtg gacttgaacg agcgctcggc ataggattcc atggagggta gtcggaggac
      781 tggttccgga gccgaccttc ccttctaaca gctgccactt tcgctgttta ggaagacttt
      841 ggtatattga ggtcttggac tcgagcgatt ttccgcggag agcaggtccg atcaaggtga
      901 ggatggatcg ctcgcgggta acaatcccag cacgaggcaa tacgaaacgg cgatcatgaa
      961 cgacaaaact ccggcggtcc cagggcagga tccgccagtc tattccgaaa gtctgctttc
     1021 tcgtaccaag gccggtccgg cggccgtggc attggtaatc ggccttgccg cgctgaccgc
     1081 acaggcttca gagacggatc tggtacgcga tattcagcgt tactgcacgg tctgctggcg
     1141 caacgccagg cttgacccac gactctggga cgactgcacc caggaagtct gctgccgcct
     1201 tcttggcaag gcgcgtattg gccaacttga cctgacccag gtcatggccg acgacacgcc
     1261 cgagcggcgc gagctggtgc gggcgatcga catggtgcgc aagcgcgtgc agcggacaaa
     1321 gaaatatcag cctctcgaca catttacgac gcccgttacc gatcttgatc agcggcaacg
     1381 gaatcgacta gagctgggtg agatccttga ggaagcccgc cgcgctgttc tgaccgcccg
     1441 gcaggatcgt attatcgagc tttggatgcg cggttggtcg gtgcccgaga tcggggccga
     1501 attgaagctg ccggtcacgc gagtcagtga cgagaaatac aaagccctgc gcaagctcga
     1561 gcatcagctc agcaatcgcc gcgaagagct ggaattcggc ctgccgagca tggcgttggc
     1621 caatctcgac tgaacgatgc ttggtttggg gcgccgtatc atcgcgagcg ggggcgtagt
     1681 tgtcagcccg ctttgatcgc cgcgagaacg gccgagctgt gaccgtcgac cttcactttg
     1741 ggaaaaattt tggcgatctt cccttgccgg tcgatcacga aggtcgtgcg ttccacgccc
     1801 atgtacttct tgccgtacat gctcttttct ttccagaccc cgtaggcttc actcaccttc
     1861 ttgtcgttgt cagccagcag ggtgaacggc agctcaaatt tcttggcgaa cttggcatgt
     1921 gaatcactgt cgtcgggact gacgccaaga atgatggcac cggctttctt gtagtcggcg
     1981 aaggcatcgc gaaattcgca cgcctccttg gtgcagccag gagtgtcgtc cttgggatag
     2041 aaatagagga cgacggacga acctttcagc ttcgacagcg tcaccttttt gccgccttgg
     2101 tcggccagcg tgaagtcagg ggccggcttg ccttcttcga ccatcgattg attctccaag
     2161 aaaacacgtt tggaatcccg gggtcggtcg gggaacaatt acaccagagc gccgcacaat
     2221 gtgacggtcg accattatcc gcttcgttct atccgtccga ggcgcggacg gaaagccatc
     2281 ttgccgcgtt tttctggcga cattgtatcc gacgtggttt gaacactggt aatcgccatc
     2341 gaggcaccga catgggccgt tccgaaacat tcgagcacac ggccgacctg gggctgagga
     2401 ttttcgcgac cgacctgccg gacctgttcc ggacggccgg cgaggcactc ttcgacgtga
     2461 ttgtcgccaa tcgcagtgac gtccgggtcg tggagaccga gctggtctca ttaacggcgg
     2521 aatcgatcgc agacttgctg gtccaatggc tcaatgagtt gatctttcgg tgcgagacgc
//
LOCUS       DATAQL010000175         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3377145, whole genome shotgun sequence.
ACCESSION   DATAQL010000175 DATAQL010000000
VERSION     DATAQL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377145"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..467)
                     /locus_tag="VHS97_02870"
     CDS             complement(273..467)
                     /locus_tag="VHS97_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447164.1"
                     /translation="MRYGTSIVDAYRHAGIYTGRLLRGEEAADLPVQQAVKVELIINL
                     KPAVALGLKFRRPCSPAPMR"
     gene            complement(548..1708)
                     /locus_tag="VHS97_02875"
     CDS             complement(548..1708)
                     /locus_tag="VHS97_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEX3447165.1"
                     /translation="MKRRLAFALLAAAFTGDSFAADLPLAGLPQLYYPGRLLPVEWTG
                     LYFGVNAGYGWANESSTIFFTGFTGGATTPAALGGTGISASGQGVTELGGTHVVGSSS
                     PRGGIAGGQFGFNWQYGMIVFGAELDAQWSGQQATASVVCTNCTASSTLKIKSLTTGR
                     ARVGLAFDWILPYVTAGGALVNAEDNLAVTVGGVTGNFQGLSGTSLGWAAGAGFDVAL
                     SSNLSARLEYLYIRADGVRLTGPIPGILGTGTASESGGYRDNILRVGLNYRFGPRGGP
                     GVLEPTVSPTAYAINYDFLPSVVLADKAKPAKQVQTMSPPIVTADAAPETSSIALPSS
                     SAKSGFKNFNEIGALEDTDTIALPKSSSKNRREKEEDESQRLKRIMAICAGC"
BASE COUNT          438 a          579 c          523 g          381 t
ORIGIN      
        1 atcgaggcat tcgggcaaca aagtcgactg tccgcgatcc gccccaacca tgaagcgctt
       61 catccgatcc tcccgcaaat cgcgcgagaa tcttaccgcg agaatcaaat ccagcgatgc
      121 gttttcacgc agccagggtc aacctcagcc attggcgtga tgtccgctgc acgaccgctc
      181 ttccaccaag aacagacatt cgccggcacg catagaacgg ccgtttcgtg ccaataccgg
      241 ccgtgagcag atgcagcaaa aaatttactc gatcacctca tcggcgcggg cgagcaaggt
      301 cggcggaatt tcaagccgag tgccacggcg ggcttgaggt tgatgatcag ttcaactttc
      361 accgcctgct ggactggcag atcggccgcc tcttcgcctc ttagcaggcg gccggtgtag
      421 atgcccgcat gtcgataagc atcgacaata ctggtcccat acctcatcga cccacccgtg
      481 cacacgatga gcgtacggtg tcccggtgct gccggcactt gccagaccat agaaaatggc
      541 gagagcccta acagcctgca caaatagcca tgatgcgctt caaccgctgg ctctcatctt
      601 cttctttttc tcgccgattc ttggaagacg acttgggaag cgcgatcgtg tccgtatcct
      661 caagggctcc tatttcgttg aagtttttga aacctgattt ggccgaactt gacggaagcg
      721 caatcgaaga ggtctcgggc gcggcatctg ctgtaacgat aggtggagac atggtctgta
      781 cctgcttggc aggttttgct ttgtccgcca ataccactga gggtaagaaa tcgtagttga
      841 tcgcataggc agttggggaa acggtgggct cgagcacgcc agggccgccg cgaggaccaa
      901 acctgtagtt caagccgacc cgcaggatat tgtctcgata tccgccggac tctgacgcag
      961 tccctgttcc caggataccc ggtattggtc ccgttaaacg gacaccgtcg gccctgatgt
     1021 aaagatactc gagtcttgcc gacaagttgc tcgacaaggc gacgtcgaag cccgcgcccg
     1081 ccgcccatcc gagcgaggta cccgatagcc cctgaaaatt gccagtcact cctccaactg
     1141 tcacagccaa attgtcttca gcgtttacca gcgcgccacc tgcagtcacg taaggaagta
     1201 tccagtcgaa ggccaggccc acacgagctc gtccagtggt aagcgactta attttgaggg
     1261 ttgagcttgc agtacagttg gtacagacaa ctgaggcggt ggcctgctga cccgaccact
     1321 gagcgtcaag ctcagcgcca aagacgatca tcccatactg ccagttaaag ccaaattggc
     1381 ctccggcaat gccgccgcgt gggctgcttg agccaaccac gtgggtgcca ccgagttccg
     1441 tcacgccctg ccccgacgcg gagatccctg tcccgcccag agcggcagga gttgttgcgc
     1501 cgcccgtaaa gcccgtaaag aaaatagttg acgactcatt ggcccaacca taaccggcat
     1561 tcactccaaa atagaggccg gtccattcga ctggaagaag acgacctgga tagtagagct
     1621 gcggtagtcc tgcgagcggc aaatcggccg cgaaagagtc gccggtgaag gctgcagcta
     1681 agagcgcgaa cgcaagccgc cttttcatag gatgccccct gccgagtctc gcgcattcta
     1741 gtttgaaggg ctaactttga ccgtgacaga tttgtcacat cccaaccgaa aaaatcacac
     1801 gtcaactgct ggccaatgca ggaccgatcg atgcgccgtg ggcgcgctga tgcggcagat
     1861 ggggtcagaa tcggtcttcc gacgatgtcc gccgcacgac cgcgttcacc ttataagcgg
     1921 a
//
LOCUS       DATAQL010000176         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_324220, whole genome shotgun sequence.
ACCESSION   DATAQL010000176 DATAQL010000000
VERSION     DATAQL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324220"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VHS97_02880"
     CDS             complement(<1..462)
                     /locus_tag="VHS97_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3447166.1"
                     /translation="MQADAYGRARQLLSSRREYVIARVLGFIQSLLLVALLGLVALFV
                     ALLASRGETRFPDSKVGLLPVWVVNRATGVDQPRADGKNPPAKPPQYLLFDDTGIFPL
                     IADNLESKNPVHRAGASILGWATRSFPALQYNRSALTTLFALGSVCIVLIAL"
     gene            complement(654..1472)
                     /locus_tag="VHS97_02885"
     CDS             complement(654..1472)
                     /locus_tag="VHS97_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3447167.1"
                     /translation="MKSLTIALATAKEAIRQPAFFVMAVFAGALLIVTVFVPYFTFGE
                     DMKMYKDTGLTTISFFCMLLALLTASSTVADEIEGKTAITLLSKPINRRQFIVGKFMG
                     IELGVLALYLILGTLFAAGVWYKYSYDLRESAGTPEAGKQWAQVTQVMPGLVLGFFEV
                     TVLTSISVAISTRLPMLANLVVCILIFFLGHLSPVLVELEKQGRVNELVGFMAKFFAW
                     ALPSLEFFNASPSISTGAVIPWLGYVLPVLGYCVLYSGAALLFAFLLFEDRDLA"
     gene            complement(1588..1866)
                     /locus_tag="VHS97_02890"
     CDS             complement(1588..1866)
                     /locus_tag="VHS97_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX3447168.1"
                     /translation="MPRVRRRILFSGRVQGVGFRATCQSLARGFDIAGYVRNLPEGRV
                     ELVAEGEGSEIDAFLTTVQREMSRYIANMTTNPEPPGDGSLIGFSIRH"
     gene            complement(2155..>3508)
                     /locus_tag="VHS97_02895"
     CDS             complement(2155..>3508)
                     /locus_tag="VHS97_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447169.1"
                     /translation="GLAAGLVGGLTLIGASNTTAQTKTPTAADPESSAGPVAVATETV
                     NLLTASKAGDLDVAARGQGQERVHLSIKNRSARRLNVIVPPGLVAASTVGQGGAGGRG
                     GGGMQSMGLGSAANREGAFGEFQLAGSPEGLRSVGVADETRTRQITVPVGETIELNIP
                     AVCLNFGWATPTPRDKFSLMDVEEYSPNPRVRKALRSLATYGTSLGVAQAVMWRVCND
                     LPFETMVEQAGKVMNVHEIALAGRFVEALDSSSTGDLVDLATVRDSRVFVQVRGEGSL
                     GSDAQRLAGQLDGLHILGLPLQVVESEEPPAALAPAIFLKVFVTDAKNGESRFAMVVN
                     SCSGPNAWQPLGKVSFRDNSSISVLDGQALTKAIDRAIAGSFVTVKPARRTLGTTTLK
                     VENRLPFTISNLVVRAGTSSGAPPVPFEGVGVGPARSALLPIQAATASLVEKIEINGL
                     "
BASE COUNT          807 a         1078 c         1006 g          617 t
ORIGIN      
        1 caaggcgatc agcacgatac aaaccgaacc cagcgcaaac aaggtcgtga gtgcgctgcg
       61 attgtattgg agggccggaa aggaccgcgt cgcccatccc aggatcgacg ctcccgcccg
      121 gtgcacggga ttcttacttt ccaggttgtc ggcgatgagc gggaaaatgc ccgtgtcgtc
      181 gaagagcaga tattgcggcg gtttagccgg cggattctta ccgtcggcgc gtggctgatc
      241 cacaccggta gcgcgattga cgacccagac cggcagcaaa cccactttgg aatccggaaa
      301 ccgcgtctcg cccctcgacg cgagcagcgc gacaaagagt gcaaccagcc ccagaagcgc
      361 gaccagcaag agcgactgaa taaaaccgag aacccgtgcg atcacgtact cgcgtcggga
      421 gctcaagagc tgacgtgcgc gaccgtacgc gtcggcttgc atgcgtgaga tcccctgact
      481 ccgagcccca gcgatttggt tcgatgtgga ccagtaatgt cggttttgcg atctacaaca
      541 ggccgaacgc gcaggcaatc agcccgatcg acctcaaaga ccaccggttt gctaacccgc
      601 gagcccacgg cgcgaatccc ggctgggcac tcttcatcag gcaccaggtc ggatcaggcc
      661 aggtcgcggt cctcgaacaa cagaaaggca aacaataacg ctgctccact gtacaacacg
      721 caatagccaa gaacaggcaa aacgtagcca agccatggta tgactgcacc ggttgagatc
      781 gatggactgg cgttgaagaa ctccagtgat ggtagagccc atgcaaagaa cttggccatg
      841 aaaccaacca gctcgttgac gcgcccctgc ttctccagtt cgaccaagac ggggcttaaa
      901 tgccccaaga agaagatcag aatgcagacc acgagatttg ccagcatggg caatcgcgta
      961 gaaattgcga cgctaatact tgtcaaaacg gtcacttcaa aaaacccaag cacaagccca
     1021 ggcatgactt gcgtcacttg agcccattgc ttgccagcct ctggggtccc ggcggactcg
     1081 cggaggtcgt acgaatactt ataccagacc cccgcggcaa agagagttcc gagaatgaga
     1141 tagagcgcca gcaccccaag ctcgatcccc ataaatttgc ccacaatgaa ctgacgacga
     1201 ttgatcggct tggacaagag cgtgatagcg gtcttaccct cgatctcgtc ggcgaccgtc
     1261 gagctggcgg tcaaaagcgc caggagcatg caaaaaaacg agatcgtcgt cagccccgtg
     1321 tccttgtaca tcttcatgtc ttcgccaaaa gtgaagtaag gcacgaagac tgtgacaatc
     1381 aaaagagcgc cggcaaatac tgccatcaca aagaaagccg gctggcgaat cgcctctttt
     1441 gccgtggcaa gagcaatagt gagtgatttc atggcttgtg ctgatgattc cgtgaatgag
     1501 accagatcag gtaatacggg cagtcgcgat atcctcgggc gcgaatccga cgtcagcctg
     1561 ccgttatcga gtgtcaaagc ggtgaatcta gtggcgaatg gaaaacccga tcagggatcc
     1621 gtcgccaggt ggttcagggt tagtcgtcat attcgcaatg tagcgagaca tttcacgttg
     1681 gacagtggtc agaaacgcgt cgatctcgct accttctcct tcggcaacaa gctcgactcg
     1741 gccctcggga aggttgcgga catagcccgc aatatcgaaa cctcgagcca gagactggca
     1801 tgtggcgcga aaccccacac cctgaacccg gcccgagaac aagatccgcc ggcgaacccg
     1861 aggcatgaga cttcgccccg cgccggtggc gacgcggcca ctgtcaaaac aaacaaggat
     1921 cgatggcaaa cgactgccgc gtttccataa caatcgtagc cgcccggacc ttaccgaggc
     1981 aagtcattct aacggcacat gctcggaaat gtcacattcg ccagaaaaac tcggcgccga
     2041 tcgtctcaag tcgacgagca gcgccgatcg ctgtttcggc tgtcaaatgc gagcgggccc
     2101 gaggatgtgg tcgatgacac gatccccgac gagcggtttg gtccgccgtc cgtgtcaaag
     2161 accgttgatc tcaattttct cgacaagaga cgctgtcgcc gcctgaatcg gcaggagggc
     2221 agaccgagcc ggccctacgc ccacaccctc aaacggcacc ggaggagcgc ccgaggacgt
     2281 tccggccctc accaccaaat tgctgatggt gaatggaagt cggttttcga ccttgagcgt
     2341 cgtggtaccg agtgtgcgcc gcgctggctt taccgtgacg aaagagccgg cgattgcccg
     2401 atcaatcgcc ttggtcagcg cctgcccgtc gagaacgctg atcgaagaat tgtcacggaa
     2461 cgagacctta cccagcggct gccacgcgtt gggcccagag cacgaattaa cgaccatggc
     2521 gaaccggctc tcgccgttct tggcgtccgt cacgaacact ttgagaaaaa tcgccggggc
     2581 cagggcggcg gggggctctt cggattccac cacttgaagt ggcagtccca gaatgtgcag
     2641 accgtcaagc tggccggcca gccgttgggc atcgctgcct agcgatcctt caccccgaac
     2701 ttgcacgaaa acgcgtgagt cacgcacggt ggcaaggtcg accagatctc ccgttgagga
     2761 agagtcaaga gcctcaacga atcgacctgc cagcgcgatt tcatggacgt tcatgacctt
     2821 gccggcttgc tcgaccatgg tctcgaacgg caggtcgttg caaacgcgcc acatgaccgc
     2881 ctgagcaacc ccgaggctgg tcccatacgt cgccaggctg cgtaacgcct tgcgcacccg
     2941 aggattgggg ctatactcct cgacgtccat caaactgaat ttatcgcgcg gcgtcggggt
     3001 agcccaacca aaattcaggc acacggcggg gatgttcaac tcgattgttt cacccacggg
     3061 cacggtgatt tgacgggtgc gagtttcatc cgcgaccccg accgaccgca acccctcggg
     3121 actaccggct agctggaatt cgccaaatgc accctcccga ttggccgcgg atcccagccc
     3181 catgctttgc atgccgccgc cgccgcggcc gccggctccg ccctggccga ccgtgctggc
     3241 ggcgaccaag cccgggggca cgatcacatt gaggcggcgg gcggagcggt ttttgattga
     3301 gagatgaacc cgctcctgac cctggccacg tgcggccacg tcgagatcgc cggctttgct
     3361 ggcggtgagc aggttgaccg tctccgtcgc caccgcgacc ggaccggctg aggattcggg
     3421 atcggccgca gtcggtgttt tcgtctgggc agtcgtgttc gacgccccga tcaacgtcaa
     3481 gccaccaaca aggcccgcgg ccagcccg
//
LOCUS       DATAQL010000177         5673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3836328, whole genome shotgun sequence.
ACCESSION   DATAQL010000177 DATAQL010000000
VERSION     DATAQL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836328"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..381
                     /locus_tag="VHS97_02900"
     CDS             166..381
                     /locus_tag="VHS97_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447170.1"
                     /translation="MLQGCQGVEAQHVERIEHVVVGEKRLHEVPREGFLDLYISKGHN
                     GSLDGIGLKEHPSGLGLVKTVFVVPTE"
     gene            1145..1534
                     /locus_tag="VHS97_02905"
     CDS             1145..1534
                     /locus_tag="VHS97_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX3447171.1"
                     /translation="MIRNTKQRDAIRRVIQEAGRPLGAAEVLTAAALYVPGLGIATVY
                     RTLNTLLDDGSVVAVPIPGEPPRYESTSAAAHHHHHFHCGGCGRVFDVLGCPKGLDKL
                     APPGFSISGHDILLHGQCSDCRGPSGS"
     gene            complement(1531..1767)
                     /locus_tag="VHS97_02910"
     CDS             complement(1531..1767)
                     /locus_tag="VHS97_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447172.1"
                     /translation="MFPLFANRDAWGLSHSSRPISHTQLGLLVETFIVCYLGLLLFLV
                     AYVGFCALRRHIADRRRSTKSQTDRDGWDDDFFD"
     gene            complement(2045..2503)
                     /locus_tag="VHS97_02915"
     CDS             complement(2045..2503)
                     /locus_tag="VHS97_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447173.1"
                     /translation="MIREIHENLFYTSDRLLGASSGVTAQNHANHVISWLRHTILVGS
                     LILLHGAITVVGGALHALPGCGHDVSAVHHPIGSDGGLGTHCPDLGPVGAAHCSLCHY
                     LFQGQVLSEVVRVQSAYLALPHTIRHDLTSPTLQNHPTSHPRAPPADSVG"
     gene            complement(2613..>5673)
                     /locus_tag="VHS97_02920"
     CDS             complement(2613..>5673)
                     /locus_tag="VHS97_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX3447174.1"
                     /translation="AFPDTTPNQVQINTVAPALAPEEIERQVTLPVELSLGGMKGLQE
                     VRSVSKFGLSQVVAIFADEVDVYFARQQINERLGDLRLPQGIERPTLGPVATGLGEVY
                     HYYLSSDTHDLTELRTLHDWVVRPRLRRVPGVAEVNTLGGLAKQYEAQVDPAKLAKYG
                     LTFDEVTRALRENNANVGGGPVERSGELTIVQGIGVARSVAEIGGIVIKSVEGVPIRV
                     LDVAEVGVGHAIRRGGTTAEGKGEVVLGLAFMRMGENSRDVTQALDAAMDEVRGLLPP
                     GVEVNIVYRRTDLIGQVLRTVERNLLEGAVLVVAVLFAFLGDLRAGLIVASAIPLSML
                     FAVSMMQRVGIAGSLMSLGAIDFGLVVDSSVVMVENCVRCLSRDRTLKSKLDVVRDAA
                     IEVRKPTMFGELVIMIVYLPILTLEGVAGKMFRPMALTVIFALGASMVLSLTLMPVLA
                     SFGLPRRMSEKETWFDRLAHRLFRPVLHQALRWPVLTLCAVAVATVGATALGMTLGSE
                     FIPRLNEGTIVFDTVRLASVNLDESQRYGMRIEQIIKEEFPDEVAVVWTRTGTADVAT
                     DPMGLEISDVFVALKPRSNWKRAKSQEELVDEMSRVTAALPGMKVVYSQPIEQRISEM
                     IAGIHADLGIKLFGDDLNVLTEKAADIERVVKRIPGAAEVVSEQVTGLPVLRVEIDRQ
                     ALSRYGVSARQVLDAIAAVGGLGVGEVREGERRFPLMVRLPARYRDDPGSLRRILITT
                     AVGQRLPLTQVVRLVPTVGPSTIHREWGRRRTIVQANVRGRDVGSFVKEAQSRIAQEV
                     KLPPGYTVEWGGKFESMIRAERRLYQIVPVALALTLSLLYLSFGSLRDALMIFSGVFF
                     ARIGGVFGLWVTGQPFTISAGVGFVALAGASILEGLILVSAIRDRMAHGVPKREAIEQ
                     ARLSRLRPVLMTGMVASLGFVPMMLSTGIGAEVQRPLATVVVFGMACDTIMTMMALPA
                     LYLLFGKEPRSMTKAEDSAPRPDLAPLASNGDGTEPRRQSVLVGTQG"
BASE COUNT         1142 a         1768 c         1786 g          977 t
ORIGIN      
        1 cctgatgtgc ctccgtcatc cttcgagact ccgggaagtc atgattaacg agggttccga
       61 gaccgtccgc gcggtccgga tacggcgtga gaggcccctt cggccccatc tcaagccgag
      121 gaacgcgacg actacgagtg ccgcgtaaat cgcgaacgca agggcgtgct ccaagggtgt
      181 cagggggttg aggctcagca tgtcgagcgc atcgaacatg tcgtggtggg cgaaaagcgg
      241 ctgcatgaag ttcctcgcga agggttcctg gatttatata tttccaaggg acataacggt
      301 tcccttgacg gaatcgggct caaggaacac ccatccggct tgggactggt caagacggtt
      361 ttcgttgtcc cgacagagta agggggaaga cccgtctagc aagcggatgg gcgactcgcc
      421 ccgcctggat tcgccgaatg acaactacgc aacgcgcggt gttccggcgg caagccagcg
      481 cgatgttgtc gtcgaggccg accaaggctt gaccgcccca accgcggagg cccagcaagc
      541 ggccaggact gatgatggaa tcgctctcat cacggtcact gtagcgcaga ccggcggccg
      601 gcacggagga accgcgatta gacctgagaa tcagagggag gggctcgaca gccgagcgta
      661 cgttgagggc gaatttcggg agagatgggg gagagaaccg tgacggacgg caccgacgcg
      721 gcccgcgagg cgatgacaac tttttcagcg accacctgtg cttgcgccag gtattcgcaa
      781 accggacacg gcgaggcctg atcgacccta caagacactg ccttaagaca gtcgtcgagc
      841 gagtgcaccg tccggtcggc caccatttga tgcccgcatc ccgtgagact gtgaaggccg
      901 ggaccgagca acatgaccgt cccgttcagg aaggtcatca ggccaatcag gagagttgat
      961 aattcgcgcc gcgccattgt tagacgagtg actcacgaaa ggctgaaggt tggaatctct
     1021 ggaccgggct gccttgcttc cgacgtcgca gctacagttg actccgctcg tgataaagca
     1081 ttatcattaa cgatacgagt atgtcaagag tgaggccgaa gtaaatcttt ccgaccagta
     1141 ttgaatgatc cggaatacaa agcaaagaga cgcaatccgt cgcgtaatcc aggaggctgg
     1201 ccggccgctg ggtgcggccg aggtgttgac cgcggccgcc ctctatgtgc ccggtctcgg
     1261 catagcgacg gtctacagga ccttgaacac cttgcttgac gatggctccg tggtagcggt
     1321 gcccatcccg ggcgagccgc ctcgatacga gagtacgagc gcggcagcgc accatcatca
     1381 tcatttccat tgcggcggct gcggacgggt cttcgacgta ctcggctgtc caaagggtct
     1441 ggacaagctg gccccgcccg gcttctcgat cagcgggcac gacatattgc tccatggtca
     1501 atgttcggac tgcagggggc catcgggctc ctaatcaaag aaatcatcgt cccatccgtc
     1561 acgatcggtt tgtgacttgg tggatcgccg gcgatcggcg atgtggcgcc gcagggcgca
     1621 gaaaccgacg tacgcgacca ggaacaacag cagtcccaaa taacacacaa tgaatgtctc
     1681 gacgaggagc cctagctgcg tgtgcgagat gggacgggat gagtgggata gcccccacgc
     1741 atcacggttg gcaaagagtg gaaacatgaa ggccttccac gcgttgaatg tggcgctgct
     1801 ttcagccaga gacgtgctgt ttcgtgggtt agcaaaagtc ggctcaatcc ccggggatcg
     1861 gaggtccgat ggacttggcg ggccggttcg cgaccgagag agcaggcgaa cgggtcacct
     1921 gcggccggcc gaaaaacggg ccgcctcggt ccaatcggcg cgagcgtggg acagacccca
     1981 cgggctgttc cggtagacag cccggaccgt ccccaatgat ccggccgatg tccgaaccac
     2041 tgcgttatcc cacggaatcg gctggaggtg cacgcggatg ggaagtcggg tgattctgga
     2101 gcgttggtga agtgagatcg tggcgaatgg tgtgaggaag tgccaggtat gcggactgta
     2161 ccctgacgac ctcggagagg acctgccctt gaaagaggta atggcacaag gagcaatggg
     2221 cggctcccac cggacccaga tcgggacagt gagtgccaag acctccatcg gaaccgatcg
     2281 ggtggtggac agcggaaacg tcatggccgc atccaggcaa cgcgtggaga gcgccaccga
     2341 cgaccgtgat cgctccgtgc aggaggatca ggctaccgac caggatggta tgacgcagcc
     2401 aagaaatcac gtgattagca tggttctggg cagtgacgcc cgaggaggcg ccgagaagcc
     2461 tatcggacgt atagaaaagg ttctcatgaa tctcgcgtat cattccccga tcaatcgacg
     2521 gcgtcaatgt cgctttcgag aagggaggag cctcgccgag ttgaggatga gcccgatggt
     2581 cctgccagac cgaccctgat gcctctttgc cctcaccctt gagtcccgac caaaacggac
     2641 tgccgccgcg gctccgtccc gtcgccgttg gaggccaacg gcgcgaggtc gggccgcggc
     2701 gcggagtctt cagcctttgt cattgaccgt ggttccttcc cgaataggag atacagggcg
     2761 ggtagggcca tcatggtcat gattgtgtca caggccatac cgaagacgac gacagtggct
     2821 agtgggcgct ggacctcggc gccgatcccg gttgaaagca tcatcggtac gaatcccagc
     2881 gacgcgacca tgccggtcat cagcaccggc cgcagtcggg agagccgcgc ctgttcgatc
     2941 gcctcgcgct tgggcacgcc gtgggccata cgatcccgga tggcactcac taggatcagg
     3001 ccctcgagta tcgaggcgcc ggccagggcc acgaagccga ctccagccga gattgtgaat
     3061 ggctggccgg tgacccaaag gccgaagacg ccaccgattc gcgcgaagaa gacgccgctg
     3121 aagatcatga gcgcgtcgcg aagcgagccg aacgacagat agagtaggct cagcgtcagg
     3181 gccagggcga ccggtacgat ttgatagagc cgccgctcgg cgcggatcat gctctcgaac
     3241 ttaccacccc attcgactgt gtagcccgga ggcagtttca cctcctgcgc gatccgggac
     3301 tgcgcctcct tgacgaacga tccgacgtcg cggcctcgga cgttagcttg cacgatcgtc
     3361 cgccgcctgc cccattcgcg gtggatcgtc gatgggccga ccgtgggcac cagccgaacg
     3421 acctgcgtaa gtggtagccg ctgcccgacg gcggttgtga tcagaatgcg gcggagcgag
     3481 ccggggtcgt cacggtatct cgccgggagc cgcaccataa gggggaagcg acgctcccct
     3541 tcgcggacct cacccacgcc gagcccaccc accgccgcga tcgcgtcgag cacctgtcgc
     3601 gccgacacgc cgtagcgtga gagggcctgc cggtcgatct cgacccgaag gacgggaagc
     3661 ccggtcacct gctcgctcac cacctcggct gcaccgggga tccgtttgac gaccctctcg
     3721 atgtcggcgg ccttctccgt gaggacgttc aggtcgtcgc cgaacaactt gatccccagg
     3781 tcggcgtgga tgccggcgat catctcgctg atacgttgct cgatcggctg actgtaaacc
     3841 actttcatgc cgggcaaggc cgcggtgacc cgcgacatct cgtcgaccaa ctcctcctgg
     3901 ctcttcgcac gcttccagtt cgagcggggc ttgagcgcga cgaacacgtc gctgatctcc
     3961 agccccatcg ggtcggtggc cacgtcggca gtcccggttc gggtccagac cacggccacc
     4021 tcgtctggga actcttcttt gatgatctgc tcgatccgca tcccgtaccg ttgcgactcg
     4081 tcgaggttga cgctggcaag ccgcaccgtg tcaaagacga tcgtcccctc gttgagccgc
     4141 gggatgaact ccgagcccag tgtcatccct agcgcggtag cgccgacggt tgcgactgcg
     4201 acagcgcaga gagtcaggac cggccaccgc aaggcttggt gaaggaccgg tcggaatagg
     4261 cggtgcgcca ggcggtcgaa ccaggtctcc ttctccgaca tccgccgcgg cagcccaaac
     4321 gacgccagca caggcatgag ggtgagcgac aagaccatcg acgcccccag tgcgaagatg
     4381 accgtcagcg ccatcggccg gaacatcttc cccgccacgc cctccaaggt caggatcggc
     4441 aggtagacga tcatgatcac aagctcgccg aacatggtcg gctttcggac ctcgatcgcc
     4501 gcgtcgcgga cgacgtcgag cttcgacttg agcgtacggt cgcgggaaag acagcggacg
     4561 cagttctcga ccatcacgac cgagctgtcg acgacgaggc cgaagtcgat cgcccccagg
     4621 ctcatcaggc tgccggcaat ccctacgcgc tgcatcatcg acacggcgaa aagcattgac
     4681 agtgggatcg ccgaggcgac gataagtccg gccctcaagt cgccgaggaa ggcaaagagg
     4741 acggcgacca cgagcaccgc cccttcaagt aggttgcgct cgactgtccg gagtacctgg
     4801 ccgattagat cggtgcgcct gtagacaatg tttacttcaa cgcccggggg cagaagcccc
     4861 cggacctcgt ccatggcggc atcgagcgcc tgcgtcacgt cacgcgagtt ctcccccatc
     4921 cgcataaagg ccaggcctag gacgacctca cccttgccct cggcggtggt gccgccgcga
     4981 cggatcgcgt ggccgactcc gacctcggca acgtcaagga cccggattgg gaccccttcg
     5041 acggatttga tcacgatccc accgatctcc gcgaccgacc gagccacgcc gatgccctgc
     5101 acgatggtga gttcgcctga tcgctcgacc ggacctccgc cgacgtttgc gttgttctca
     5161 cgcagggcac gggtgacctc atcgaatgtc aacccatact tcgccagctt cgccgggtct
     5221 acctgggcct cgtactgctt ggctaaacct cccagcgtgt tgacctcggc gacacccggc
     5281 acccgcctca ggcgtgggcg aacgacccaa tcgtgaagcg ttcgcagttc agtcaggtcg
     5341 tgcgtgtcgc tgctgaggta ataatggtag acctcaccca ggcccgtggc gaccggaccg
     5401 agcgtcgggc gctcgatccc ctggggtagc cgcaggtcgc ccagccgttc gttgatctgc
     5461 tggcgggcga aatagacgtc gacctcgtcg gcgaaaatcg ccacgacctg agagagcccg
     5521 aacttcgaga ccgagcgtac ctcttgtaat cctttcatcc cccccaacga caattccacc
     5581 gggagggtta cctgccgctc gatctcctct ggcgcaaggg ccggggccac cgtattgatc
     5641 tggacctggt tgggcgtcgt gtcgggaaaa gca
//
LOCUS       DATAQL010000178         6044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2461849, whole genome shotgun sequence.
ACCESSION   DATAQL010000178 DATAQL010000000
VERSION     DATAQL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461849"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..755
                     /locus_tag="VHS97_02925"
     CDS             144..755
                     /locus_tag="VHS97_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3447175.1"
                     /translation="MLSLAVALGIGASCQAGLVVEAPNITAAPGSSGSFDVLITSTAG
                     SFGVASDTVDLSLTGLSGVSFTNVSIATATPYIYGANSATTVGSTFTFSTFPGTQFET
                     FDFILTLGAQTINSGEVFGLVNVQYSVAANATPGATGTLTFGPDTMLSDAAGNNVDFT
                     APNGSITISSSAVPEPSGMILLAVGCATVLVGSARRRFARPRC"
     gene            complement(913..1692)
                     /locus_tag="VHS97_02930"
     CDS             complement(913..1692)
                     /locus_tag="VHS97_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447176.1"
                     /translation="MRPRSIMRFRPGLEQFEAKQLLAAGSMTTHVVNTKDGSRALALH
                     SGGTSTAHTALAMSHVGSTVQDARRQPAPIGAYGFLAFRVTNTPWQTPFKLVPPFQQV
                     LVQGGQPVPGQVYNVAYVSVKNGTAQTFTASNNFTVRMTNQPRSFGGFPVLTGNEQWK
                     PGQILVFYVLTKKYYPVSFVGGGFQFNLGGRSSTAIPGPSGIFLRLKYNSATFANTLN
                     WIVAFGQGNQVGRGAQLGLPNTSINQIVDARTQRPDFAGHF"
     gene            complement(1977..3857)
                     /locus_tag="VHS97_02935"
     CDS             complement(1977..3857)
                     /locus_tag="VHS97_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447177.1"
                     /translation="MERSGRSTFVLATLLMYSLATWSCRAQYPAVPLATSVPTPRSAR
                     DDQAVKRVPMPPESIDLRHPIDFATYFRVPGESTSPTATAPRTSPPAPNPVRLAVYSE
                     PLAAPAAVPPRSAIPTPRQFGQPSPSTTPTPTPAPASDLAPPLGDLSMRLKSAPLEPT
                     DVGFPINLATALRLSDARPLIVAAAQASVWVAEAQLTRAKVLWVPQLNIGGDYIRHDG
                     GGPDFNKGLLTAPSVNFFYGGGGLWEIQALTDVFFEPLVKRQVLNSRQADIQTAKNDA
                     ALMTATTYFDVHQYRGMYAGALYCVNRGRDLVERIDSLSAELVSKVEVERAKNLLADL
                     EQQSVSSRQSWRVSSARLTRVLRLNPLAVVVPLEHDHLQITLIDPARPLPDLHVVALT
                     NRPELASYHALVEAAVARIRRERGRMLLPTVLVNGFQTPDEMIQAGIFGIGTNNKLNQ
                     WTGRDDVSLQLMWELEGLGLGNLARIKEQRGEQSRAVIDFFNIQDMVAADVTRAQADL
                     QSAATRVDQAERALRSGIATFNGSFEGLQNTTRYGDVLVLVSRPQEVVFALQLMKTAF
                     DNYFTTVAEYNKAQFDLFHALGYPAREITYVRPPGTEEPVYTTRPDYLPPVGNGPPPA
                     TR"
     gene            complement(4208..5050)
                     /locus_tag="VHS97_02940"
     CDS             complement(4208..5050)
                     /locus_tag="VHS97_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447178.1"
                     /translation="MTLKVEKNRNDAGPAQASVHDVPGRRGLPHYQERAGSARTEGSD
                     REARNRLVQANLGLVVTIARQFLGRGLQLDDLTGEGNLGLIRAAEEFNPSYGTRFSTY
                     ASLWIKEAIRRALINTTTTIRLPAHMVGLLTKWRRAERALLREAGRMPTFEEIASVLG
                     LSEVQKSLVAQALQAAQLKFEGDCSDESEIRLADAVGNRQQGTSEDVVESEDEWVFTA
                     RRMERLDARERAILALRYGLEGDLLTFKEIGRRFGMTGEWARRLEHRALSKLGDKRCD
                     CPCD"
     gene            complement(5482..>6044)
                     /locus_tag="VHS97_02945"
     CDS             complement(5482..>6044)
                     /locus_tag="VHS97_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447179.1"
                     /translation="EEAMRIKDQIVLSNLRLVVSIARRFAAPSQELSDAVSDGVVALL
                     RAVDRFDFARGYKFSTFATCVVRNEFVRAGRSSRRHDRFVGGSDGLADTADTRCDEGE
                     EERAMQDRHQAVALMLGQLKDRERRIVIGRYGLGGASKQTLVQLGNELGISKERVRQI
                     ELRATTKLRRIAQTAKNRWERECQHS"
BASE COUNT         1102 a         1809 c         1874 g         1259 t
ORIGIN      
        1 gcaacattgt gatgtccaac tttcaatcgc gctcactcaa agtcaaaggt gcaaagacat
       61 ccatgaaaaa acgtttgccc atctgcctat tttctctccc tggtgcggcc ttgtttaggc
      121 gttgcaatgg ataccttcga gtcgtgctct cactggcggt cgccctcggc atcggcgcct
      181 cgtgccaagc cggtctggtg gttgaagctc cgaacatcac cgccgccccg ggctccagcg
      241 gatcgttcga cgtcctcatc acgagtacgg caggatcatt cggcgtcgcg agcgacaccg
      301 tcgatctctc tctcaccggc ctgagcggcg tttccttcac caatgtttcg atcgccacgg
      361 cgacgcctta catctacggc gcgaactccg caacgaccgt aggatccacg tttactttta
      421 gtacgttccc aggcacgcaa ttcgagacgt tcgactttat cctcacgttg ggcgcccaga
      481 cgatcaactc cggggaagtt tttggcctgg tgaacgtgca gtattcggtc gcagccaatg
      541 ccactccggg cgccactggc acgctcacgt ttggcccaga cacaatgctg tcggacgctg
      601 ccggcaataa cgtggacttc acggctccga acggatcgat caccatttcc tcttctgccg
      661 tacccgagcc ctcgggcatg atcttgctgg ccgtcggctg tgccacggtt ctggtcggtt
      721 cggcgaggag gcgctttgca agacctaggt gctgatcgga acgaggtcga acgttttccc
      781 cgctctaatg gccagctcct ccaagatccg gaggagcaca cccacgttgg atccacatgc
      841 ctcgtcagac gttgacgcgg ctgctcaagt tggcggcatc cgtcatccta atccagcgcc
      901 gacaattagc gattagaaat gccccgcaaa atcagggcgc tgggtccgcg cgtcgacgat
      961 ctggttgatc gatgtgttgg gaagacctag ttgtgcgcct cggccaacct ggttgccctg
     1021 cccaaacgca acgatccaat tgagagtgtt ggcgaaggtg gccgaattgt acttcagccg
     1081 aagaaagatc cccgatggtc caggtatggc tgtcgatgac ctgccaccga ggttgaactg
     1141 gaaacccccg ccgacgaagg acaccgggta gtacttcttg gtcagcacgt agaagacgag
     1201 tatctggccc ggtttccact gctcattacc cgtaagaacg ggaaagccac cgaaggatcg
     1261 cggttgattc gtcattctta cggtgaaatt attgctggca gtgaaggttt gcgccgtccc
     1321 gttctttacg ctgacataag ccacattgta aacctggccg ggcaccggct ggccaccctg
     1381 gaccaagacc tgctggaagg gtggcaccag cttgaagggg gtctgccacg gcgtgttggt
     1441 tacacggaac gcgaggaaac cgtaggctcc tatgggtgcg ggctggcggc gagcgtcttg
     1501 taccgttgat ccgacatggc tcatcgcgag tgccgtatga gcagtcgatg tgccgccaga
     1561 gtggagggcc aaggcccgtg agccgtcctt tgtgttcacg acgtgagtcg tcatcgaacc
     1621 ggcggcgagc agttgtttcg cttcaaattg ctcgagacca ggacggaatc tcatgatcga
     1681 gcgcgggcgc attgatgaac gaccctcctg gttcaaggaa gcattgacgg atggaatccg
     1741 tcagtccgac ccgcaagggt ctggcatccg tctgagccgt ggcgcgatct cggttggagg
     1801 ctgggcgcta tcgccatgtt ggggcgtatg gcggcccgct cgatgatacc ctacgaatcg
     1861 gatgtcgagg cgccggcgca ttattcatgg tgcccattat cccggtcggg ccggctcgat
     1921 tattcccgcc gaatcggata cgcgggctgg tcgagcggtc aaacggtcgg tgcatgtcag
     1981 cgggttgccg gcggcggccc attgcctacc gggggtagat aatcgggccg ggtcgtgtac
     2041 acaggttcct cggtgccagg ggggcgaacg tacgtgatct cgcgggcggg gtagccgagc
     2101 gcgtggaaca ggtcgaattg cgccttgttg tattcggcga ccgtcgtgaa atagttgtcg
     2161 aaggccgttt tcatcagctg gagcgcgaag acgacttcct gcggccggct gacaaggacc
     2221 aggacatcgc catatcgtgt ggtgttctgc aagccttcaa agctcccgtt gaacgtggcg
     2281 attcctgagc gcagcgcgcg ttcggcctgg tcgacccggg tcgccgctga ttggagatct
     2341 gcctgcgcac gggtcacgtc tgccgccacc atatcttgga tgttaaagaa atcgatgacg
     2401 gccctcgact gctcaccgcg ctgctctttg atccgtgcca gattgccgag tccaaggcct
     2461 tcgagctccc acatgagctg taagctcacg tcgtcgcggc cggtccactg gttgagtttg
     2521 ttgttggtgc caattccgaa gatcccggcc tgaatcatct cgtcgggagt ctggaagcca
     2581 tttaccagga cggtggggag gagcatgcgc cctctttccc ggcggatccg cgcgacagcc
     2641 gcctcaacga gcgcgtggta cgaggcgagc tcgggacggt tggtcagggc gaccacatgc
     2701 aggtcgggga gcggtcgtgc gggatcgatc aaggtgatct gaaggtgatc gtgctcgagc
     2761 gggacgacca ccgcgagtgg attgagccgc aggacccgag tgaggcgggc gctcgacacc
     2821 cgccacgatt gccgtgacga gaccgattgc tgctcaaggt ccgcgagcag attcttggcc
     2881 cgttcgacct ccactttcga gaccagctca gcactcaaac tgtcgatccg ctcgaccaag
     2941 tcgcggccgc ggttgacaca gtagagggct ccggcataca tgccccggta ctggtggacg
     3001 tcgaagtaag tggtcgcggt catgagcgcg gcgtcgttct tggcggtctg gatgtcggct
     3061 tgccgggaat tgagcacctg gcgcttcacc aggggctcaa agaagacatc ggtcaacgct
     3121 tggatctccc acaggccgcc accgccgtag aagaaattca cgctcggtgc ggtcagcagc
     3181 cccttgttaa aatcgggtcc gccgccatcg tgccggatat agtcaccgcc gatgttcaat
     3241 tgtggaaccc agaggacctt ggctcgtgtg agttgcgctt ctgccaccca gacactggct
     3301 tgggcagcgg ccacgatcag cggccgagcg tcagagagtc gcaaggcggt ggcgagattg
     3361 atcgggaagc cgacgtcggt cggctccagt ggcgccgact tgagcctcat ggataggtcg
     3421 cccagtggtg gcgccaggtc cgaagccggc gccggcgttg gggttggcgt ggtcgatggg
     3481 ctcggttgtc cgaattggcg cggcgttggt atggcggacc gaggcggtac ggccgccggc
     3541 gcggccaacg gctccgagta gaccgcgagt cgaaccgggt tcggggctgg cgggctggtc
     3601 cgcggcgccg ttgctgttgg agaggttgat tcgccaggca ctcgaaagta ggtagcgaag
     3661 tcgatcggat ggcgcaggtc gatcgactca ggcggcattg gcacgcgctt caccgcttgg
     3721 tcgtcgcgtg ccgacctagg ggtcgggact gaggtcgcca gcgggacggc tgggtactgg
     3781 gcccggcagg accacgttgc caacgaatac atcagcaggg tggcgaggac aaacgtagat
     3841 cggccggatc gttccatcgg gcttcgctcc ttcgccatcc ctcctgatcg gaacgcgtaa
     3901 cgcggccgat caggccagac agaacggcgt aggattcatc ggctttttac gatgagaatg
     3961 ttgacgccga ttaaagcgat ttggagcctt ccgttcttat cttattgccg ggctagagct
     4021 tgtatctgcg caccgagtcc cgatttcttg cgttttcaaa tctacctctg cggtcgagac
     4081 ttcgggaggc ccgcagccaa atccgcgctg tgtgcgttcg taggggttac tcatgtcggg
     4141 ctggtgctcg aaatacccgc gttcctagcc aggccaaatc gagcaagatt tcttagtgca
     4201 ttgcatgcta atcgcacggg caatcgcagc gcttgtcgcc gagtttgctt agggcgcggt
     4261 gctcgagcct tcgtgcccat tcccctgtca ttccaaatcg acggccgatt tccttaaacg
     4321 tcagcaaatc gccttccagc ccatagcgca gcgccaggat ggctcgctcg cgggcgtcca
     4381 ggcgctccat ccgtcgagcg gtaaagaccc actcgtcctc ggattcgacg acgtcttcgc
     4441 tcgtaccctg ctgccgattc cccaccgcgt cggccaaccg gatttccgat tcgtcgctgc
     4501 agtcgccctc aaacttgagt tgggccgctt gaagtgcctg tgccaccaga gacttctgaa
     4561 cctcgctcag acccaagact gatgcgatct cctcgaacgt tggcatgcgg cctgcctcac
     4621 gaagaagtgc ccgctcggcc cgccgccact tcgtcaacag acccaccatg tgcgcaggta
     4681 agcgaatcgt tgtggtcgta ttgatcaggg cgcgccgaat ggcctccttg atccagagag
     4741 aagcataggt actaaatcgc gtcccgtagc tggggttgaa ctcctcggcc gcccggatca
     4801 atcccaggtt cccttcaccg gtcaggtcat cgagttgcag accgcggccg aggaactggc
     4861 gagcaatggt gacgaccagg ccgagattgg cttggaccag ccggttgcgc gcttcgcgat
     4921 cgctaccttc ggtccgggcg gaccccgcgc gctcctgata gtgaggcaac ccgcgccgac
     4981 ccgggacatc gtgaaccgac gcctgtgccg gaccggcatc gttgcgattt ttctctactt
     5041 tcaaggtcat cagacgcact cctaatatgt ccagtccagc gcgcaggggc cacgcttgga
     5101 ctgatcagcc tccggatcga cgacggtggt gggacaatcc tacctaacgc ttcttcaggt
     5161 gtcaccggca agcaaaaccc ttggacgcgg tgtccgtaag agtagttgag ccggtcgctt
     5221 cttcgcgcta ggtcacggac tacgtcgccg ttggggagaa ctatcggagg gtacgatgat
     5281 ctcacactgg aaatatcttc atcgcgagct tggctatcgc gatgcacacc ggaattccga
     5341 tcgtgattct gtaggagaag gtcgggccga gcacgttgcg caattcgggt ctgtggcccg
     5401 tgtccatcct ggtcaattcg tgtcgagtca accatgaccg tctccgctcg ttgcccggtg
     5461 aatctccaga cgaagtatga gctaactgtg ctggcactct cgctcccagc gattctttgc
     5521 ggtctgcgcg attctacgga gcttggtcgt ggcccgcaac tcaatctggc ggacgcgctc
     5581 cttgcttatt cccagttcgt tgccgagctg cacgagggtt tgtttgctgg ccccgccgag
     5641 tccataccgg ccgatgacaa tccgccgttc gcggtccttg agctggccga gcataagcgc
     5701 gacggcctgg tgccgatctt gcatagcccg ctcctcttcg ccctcgtcgc agcgtgtgtc
     5761 ggcggtgtca gccagcccgt cgctaccgcc gacgaatcgg tcatggcggc ggctggagcg
     5821 gccagcgcgc acgaactcgt tccggacgac gcacgtggcg aaggtgctga acttgtaccc
     5881 tcgcgcgaag tcgaaacggt ccaccgcgcg caggagtgcg acaacgccgt cggagacggc
     5941 atccgacagt tcctgcgagg gcgcggcgaa gcgcctggcg atcgagacga ccaggcggag
     6001 gttggatagg acgatctgat ccttgatccg catcgcctct tcct
//
LOCUS       DATAQL010000179         5325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_935550, whole genome shotgun sequence.
ACCESSION   DATAQL010000179 DATAQL010000000
VERSION     DATAQL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935550"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..2034
                     /locus_tag="VHS97_02950"
     CDS             307..2034
                     /locus_tag="VHS97_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX3447180.1"
                     /translation="MTINRIRKASFPLTAALALFATALPACAQGPASVVPQPREGAWM
                     EVHNSFLVLTKKGHIDLLFLGDSITQMWNENSVWRRFYEPRHAANFGIGGDRTQHVLW
                     RIQNGELDGIEPKVTVLMIGTNNLSSDTPDEIAQGIGAIVSELRRRLPKTQVLLLGIF
                     PRAPKPNAVRERLKSVNEKIAKLDDGTHVRYLDICKAFLSDDGSISRDVMPDSLHLSL
                     KGYRIWAEAMEPTLWSMLDEPKPLAAQFQREMNTPSGPGEKSEPVDITAAQIRSALAR
                     AVTPLERSLVVYAEKRDCFSCHNQTVPLVALKIARSRGVAINEDAFDGAIALTMTDLE
                     SAIGLYQKGRGQPGGATRAAYALWALETGNHSADLITATVSDFLLNFDRDLDHWTTSS
                     RRVPMEASPFTVTALTVRGLRTFGASARSNVVKERIGKARSWLAKSRPADTEDSVFRL
                     WGLNFAAAPPEETKVAAKELLAAQRTDGGWAQIDALASDAYATGSALVALHEAGGLPT
                     DDPAYRSAVAFLLRTQKDDGTWFVASRSRPFQPYFESGFPYGKDQFIAVAASGWAAAA
                     LALALPPVP"
     gene            complement(2063..2857)
                     /gene="ispD"
                     /locus_tag="VHS97_02955"
     CDS             complement(2063..2857)
                     /gene="ispD"
                     /locus_tag="VHS97_02955"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX3447181.1"
                     /translation="MGQDRFAVILPAAGKSSRFGDPKQKKIYTELDGRAVWLRAVEPF
                     VNRDDVAQIIMVIAGEDREMFERRYRASVAFMNIKVIEGGATRTESVARALEFVTPEC
                     EYIAVHDAARPCLSHELVEAVFSAARTHGAALPGVRIADTIKEAGDDHLTSRTLPREH
                     LYLVQTPQVFRRDLLFKAYANSSRFGGSVTDDCQLVEALGQQCALVEGSALNIKITTQ
                     IDLKLAAAILPLLETPKRPGSSHPYSDAQQMWSTLPKLKASDLFGA"
     gene            3100..3927
                     /locus_tag="VHS97_02960"
     CDS             3100..3927
                     /locus_tag="VHS97_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX3447182.1"
                     /translation="MMECLGPQCARMVLDWLKAQAAAAASDSSSSAATLNQGMLWSHV
                     PYQEEAPAITSLAELVGAVEHLHYAVHRRYSNQLTRAERSRLDNTIDNALFKIDRYLN
                     RAVTSDSARYVDEVHTLRQSIRDGRERLERQARLARRADLKLAQLASLSPESFEEFVG
                     ELFEMLGYEVEVVGGSGDLGADLLLRRNDGLIAVVQCKYHRQTPVGSPELQKFLGTIH
                     HTSSHKGFFVTTSTFSLSAEKFAAEHPIELVDGPRLIELVSSALDPKSRRETAPAWF"
     gene            4208..4447
                     /locus_tag="VHS97_02965"
     CDS             4208..4447
                     /locus_tag="VHS97_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447183.1"
                     /translation="MSTRTRDNTLSGLKLPDPFEDLTGVISSDLRVIATALAQRAGDR
                     LLLSPRQTHHLRRKLWNKLTQAINETMDPLTVERQ"
     gene            4497..5171
                     /locus_tag="VHS97_02970"
     CDS             4497..5171
                     /locus_tag="VHS97_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX3447184.1"
                     /translation="MARLILGVTGSVAAIRTPALYAQLNATGHDVRVVATRSSLYFFD
                     TALLGLAQGENAEVTTNGPLFRDSDEWPGTRYHRGDEVLHIEFRKWADLFVVAPLDAN
                     TLAKFALGMSDNFLTCIYRAWDFSKPLILAPAMNTLMWDSPVTLGHLRQLIEHRTTEP
                     LPAGWTLEQAPAIYAQYVPKIILIPPQSKRLACGDVGVGAMAEVTQIAEVVRHWSLQE
                     QPSADP"
BASE COUNT         1039 a         1688 c         1542 g         1056 t
ORIGIN      
        1 gagtctacgc aaaggctcgg cgagtcgcta gaggtctgtg gcaaagagct tccgggcggg
       61 ccggcaagat gctcacccct tggcccggct gccttgcggt aggttccgtt cgtcgaaact
      121 cgccaccctt tggccgtcgc acttagcggc aaagattatc cccaccgcct ggaaacagcc
      181 ccgacaagcg cagtacattc agacgtatca atcgagattc gacgcgcgaa gtccccgtgt
      241 ctcgaaacca aggactaatg accaatgact aatgaccaag gactaatgac caaggactgt
      301 tgaccaatga ccattaaccg catccgcaag gcttcgtttc ccttgacggc tgcgctggcg
      361 ctcttcgcca ccgcgttgcc cgcgtgcgct cagggtccgg cgtccgtcgt accccagcca
      421 cgcgaaggag cctggatgga ggttcacaac agcttcctcg tgctaacgaa aaaagggcac
      481 atcgaccttc tcttcctggg tgattcgatt acgcaaatgt ggaatgaaaa tagcgtttgg
      541 cggcgctttt acgagcctcg ccacgccgcc aatttcggca tcggcggtga tcggacccag
      601 cacgtcctct ggcggattca aaacggcgag ctcgacggca tcgagcccaa ggtcaccgtt
      661 ctcatgatcg gcaccaacaa cctctcctcc gacacccccg acgagatcgc gcaggggatt
      721 ggcgccatcg tcagcgaact gcgccggcgg ctccccaaaa ctcaggtcct cttactgggc
      781 atatttccgc gagccccaaa gccgaacgct gtgcgcgaac ggctcaagtc cgtcaacgaa
      841 aagatcgcca agctcgatga cggtactcac gtgcgttatc tcgatatctg caaagcattc
      901 ttgagcgacg acggttcgat ctcgcgtgac gtcatgcccg attcccttca tctgagcttg
      961 aaagggtatc ggatctgggc cgaagccatg gaacccaccc tctggtcgat gctcgacgaa
     1021 ccgaagccgc tggcagcgca attccagcgt gaaatgaaca cgccgagcgg acccggtgaa
     1081 aagtcggagc ccgtcgatat cacggccgca cagatccggt cagcccttgc gcgggccgtg
     1141 acgccgctag agaggagcct cgtcgtctac gccgagaagc gcgattgctt ctcgtgccac
     1201 aatcaaaccg tccccctcgt cgccctcaag atcgcccgct cccgcggcgt cgcgattaac
     1261 gaagatgcct tcgacggggc catcgccctg acgatgaccg acctcgagag tgcgatcggg
     1321 ttgtaccaaa aagggcgtgg ccaacccggt ggggcgaccc gcgccgccta tgcattgtgg
     1381 gcgctcgaga caggcaatca ctctgccgat cttatcaccg cgaccgtatc ggatttcctg
     1441 ctgaacttcg atcgcgatct agatcactgg accacctctt ccaggcgcgt acccatggag
     1501 gcgagcccct tcaccgtgac agccttgacg gtccgagggc ttcggacctt cggggcgagc
     1561 gcgcggtcaa acgtcgtgaa agagcggatt ggcaaggccc gttcctggct ggccaagagc
     1621 aggcccgccg acaccgagga cagcgtcttc cgcctctggg gcctgaactt cgccgccgcg
     1681 ccacctgaag agaccaaggt cgcggcgaag gagttgctcg ccgcccagcg caccgatggg
     1741 ggttgggccc agatcgacgc gctggccagc gatgcgtacg caaccgggtc cgcgctcgtg
     1801 gcgctgcatg aggccggcgg gttgccgacg gatgatccag cctatcggag cgcggttgcc
     1861 ttcttgttgc gcacccaaaa ggacgacggc acctggttcg tggcttcacg gagcaggccg
     1921 ttccagccct atttcgagag tggcttcccc tacggcaagg accagttcat tgcagtggcc
     1981 gccagcggct gggccgccgc ggcgcttgcg ctcgcactac cgcccgtgcc gtaggcagac
     2041 gacggtaagc cagcttcagg tctcatgcac caaacagatc cgaggccttc aacttgggga
     2101 gcgtactcca catttgctgt gcgtcggaat acggatgcga cgaacccggg cgcttcggtg
     2161 tttccagcag gggcagaatg gccgcggcga gcttcagatc gatctgcgtt gtgatcttga
     2221 tgttgagcgc cgagccctca acaagcgcgc attgctgacc cagtgcttcg acgagctggc
     2281 agtcgtcggt aacgctgccg ccgaagcgtg aactgttggc ataggccttg aagagcagat
     2341 cacgccggaa gacctgcggg gtctggacca ggtagagatg ctcgcgtggc agagtcctgc
     2401 tagtcagatg atcgtcgccg gcctctttga tcgtgtcggc gattcgcacg cccggcagcg
     2461 cggccccatg ggttcgggcg gcactgaaaa cagcctcgac cagttcatgg ctcaagcagg
     2521 gccgagccgc atcgtgcact gcgatgtatt cgcactccgg ggtcacaaac tcgagcgcgc
     2581 gggctaccga ctcggtccgg gtcgcccctc cttcgataac tttgatgttc atgaacgcga
     2641 cactggcacg atagcggcgc tcgaacatct cgcgatcctc gccggcgatc accatgatga
     2701 tctgggcgac gtcgtcacgg ttgacgaagg gctcgacggc ccgcagccag acggcgcggc
     2761 catccagctc tgtgtaaatc tttttttgct tcgggtcgcc aaaccgcgac gacttgcccg
     2821 cggctggcag aatcacggcg aaccggtcct ggcccatggc tccctctctt cccgagacgc
     2881 aagggatcct ctcgcactct ccgcaccccg cggcgatgct tcgccgcgaa gccgacttcc
     2941 gtgctgacgc ctctagcccc gattttgatc ctgtcctcct cgggtgtcaa ctcgggacga
     3001 gaatctcgca tgctcgcgcg gaattgtgtt gtctggttcg cgcgcctcgt acaagctgaa
     3061 gcgtctttgg tcacatggag gatttgtcgg agatcgacaa tgatggagtg tctcggacca
     3121 caatgcgcca gaatggttct cgattggctc aaagcccagg ccgcggccgc ggcaagcgac
     3181 tcttcctcat cagcggcgac tctcaatcaa gggatgctct ggagccacgt cccctaccag
     3241 gaagaagcac ccgcgatcac gtccctcgcc gagcttgtgg gggcagtcga acaccttcat
     3301 tacgccgttc acaggcgcta ttcgaaccag ttgacgcgtg ccgagcggtc tcgtctcgac
     3361 aacacgatcg acaacgcact gttcaagatc gatcgctatt tgaaccgcgc tgttacatcc
     3421 gattccgcgc gttatgttga cgaagtgcat actttacgcc agtcgattcg cgacggacgc
     3481 gaacgcttgg aacgacaggc acgtctggcc cgccgcgctg atctcaagct ggcccagctt
     3541 gcttcactgt cccccgagtc gttcgaggaa tttgtcggcg agctcttcga gatgctcggc
     3601 tacgaggtcg aggtcgttgg cggctccggc gatctcgggg ccgacctgct ccttcgccgc
     3661 aatgatgggc tcattgccgt cgttcagtgc aagtatcaca ggcagacccc ggtcggctcg
     3721 cctgagcttc agaaattttt gggcacaatc catcacacca gcagccacaa aggattcttc
     3781 gtgacgacga gcacattctc gctctcggct gagaagttcg ccgccgaaca cccgatcgag
     3841 ctcgtcgacg ggccgcgctt gatcgagctc gtctccagcg ccttggaccc gaaatctcgc
     3901 cgcgaaacgg cgccggcgtg gttttaactt cgtgacgagt tctgcggccc atcttcgtcc
     3961 cacaatgtgt tgactgatat cgtatgatag aaatagggaa atggcatgcg gcttgcgtca
     4021 ccatctgatt gggcgattga gccgagcgat ttccggcacg gcaagcccgc ccccagcgcg
     4081 gcaccaacgg agcggagatt tcctggagaa agacttgact gagggattga acacgggtta
     4141 gcttttcgat cgtgtcacgg aaaggatcca acctcccgtc tcacccttgg ccaaggaggg
     4201 ttgttgaatg tcgactcgta ctcgcgacaa cacattatca ggcttgaaac ttcccgaccc
     4261 cttcgaagac ctgacaggag tgatcagttc cgacctgagg gtgatcgcga cggctctggc
     4321 tcagcgcgct ggagaccggc tcttgctctc acctcgtcaa acacatcatc tgcggcgcaa
     4381 gctttggaac aagctcacgc aggcgatcaa cgaaaccatg gatccgctca cagtcgagcg
     4441 gcaatgacct ttcatccgac cgcgaacgtt tctcttagcg actctgaatt ttgaccatgg
     4501 ctcggctcat actcggcgtg accggctcgg tcgccgcaat ccgcacacct gcgctctacg
     4561 ctcagctcaa tgccaccggt cacgatgtcc gcgtcgtggc gacccgatct tcgctctatt
     4621 tttttgatac cgcccttctc ggcctggccc agggcgagaa cgccgaagtc accacgaatg
     4681 gaccgctctt tcgcgatagc gacgaatggc ccggcacccg gtatcaccgg ggcgatgaag
     4741 tcttacatat cgaatttcgc aagtgggccg acctgtttgt ggtcgcgccc ctggacgcca
     4801 acaccctggc caagttcgct ctcggcatgt cggataattt cctgacttgc atctaccgag
     4861 cgtgggattt ctcgaagccg ttgatactag caccggcgat gaacaccttg atgtgggata
     4921 gccccgtgac tttggggcac cttcgccaat tgatcgaaca ccgtaccacc gagccgctgc
     4981 cagccggctg gacgctcgaa caagctcccg ccatctacgc gcagtatgtg cccaaaatca
     5041 tcttgatccc gccgcaatcc aaacgcctgg cgtgcggtga tgttggtgtc ggggccatgg
     5101 ccgaggtgac ccagatcgct gaggtggtcc ggcactggtc cctgcaagag cagccatctg
     5161 ccgatccgtg agatcggcgg acggaacatt gccgggccgc ggttttctga cgatcgttat
     5221 ttggctggaa gagcgtcgat aattcggtcg atcttcttct cgagcgcgtc aagtcgtcgg
     5281 ccgagttcgg gggcaggcgg tgcgacatcc ggtgcaattc ccaga
//
LOCUS       DATAQL010000180         5203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1545903, whole genome shotgun sequence.
ACCESSION   DATAQL010000180 DATAQL010000000
VERSION     DATAQL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545903"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VHS97_02975"
     CDS             complement(<1..933)
                     /locus_tag="VHS97_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX3447185.1"
                     /translation="MANTQKYVYSFGGGSAEGRSDMKDLLGGKGANLAEMSVIGIPVP
                     PGFTITTEVCADYYERGRQLPDEVRPQVESALSRMEEAVGKKFGNPADPLLVSVRSGA
                     ALSMPGMMNTILNLGLTDASVEGLAKKTANARFAYDGYRRLIDMFGSTAMGVEHEAFE
                     HELESLKNERGVKLDTDLNGDDLKELVRRYKAVYQKHVKESFPQDPKDQLWKAIMAVF
                     NSWMGNKALEYRRIERITGLNGTAVNVQAMVFGNTGNDSGTGVAFTRDQNTGADEFYG
                     DYLINAQGEDVVAGIRTPEPISRLKEEMPKVYDQL"
     gene            complement(1174..2061)
                     /locus_tag="VHS97_02980"
     CDS             complement(1174..2061)
                     /locus_tag="VHS97_02980"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HEX3447186.1"
                     /translation="MSQFESNVEILVSPALPGAGRVSMKTALKRGVIVAASLAMAASV
                     FVGARSLWLEWALLKDAVGGAQASALVGFRDIAPTVSYAQPPRDLIRQEGNETLLWSR
                     WENGVGHHWFHFRRGDIDPAHLRHPESTMISRAIDHPVVESNGGTIWKRIPQECTVVG
                     HRLVGLRCAYPVIVLGKVQVINDVVEDHPYLIVANLFAPNQEAYSIFESELDGHRVTM
                     AASGYFHDGKPLLYDRGTESLWLEEADHLKAAAGKHKGARLVRVARPAPVSWKSWLAQ
                     SKDCRLLVGADRSQATPRE"
     gene            complement(2363..4456)
                     /gene="fusA"
                     /locus_tag="VHS97_02985"
     CDS             complement(2363..4456)
                     /gene="fusA"
                     /locus_tag="VHS97_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX3447187.1"
                     /translation="MENLRNVRNIGISAHIDSGKTTLTERVLYYAGRTHVIKEVKGEG
                     AVMDHMELERERGITITSAATTVQWNETKINIIDTPGHVDFTVEVERSLRVLDGAVLV
                     LCAVAGVQSQSITVDRQMKRYAVPRIAFINKMDRTGANPANVIQQLETKLGLTAIPLQ
                     LPIGAESSFLGVIDLIERHAVYFDGDKGESIRNEPIPADMVEAAERARQGLLEALSMI
                     SDQIMELLLEEAEVPIDLIHKTIREGTIAQQLCPVMVGSAYRNKGVQSLLDAVCRYLP
                     SPLDREIFAKDHTNGGAEVPLAPDPDAPLVSMAFKLVEEPFGQVTFVRIYQGTLSKGT
                     FYFNSRQRKRARISRILRVHADQKEDIDSASAGDIVAVMGIECATGDTYCAEGTDLSL
                     ESIYAAEPVIDLSIQPLKRADYDKLAKALHRFMREDPTFRVHVDHETSETIISGMGEL
                     HLEIYVERIRREYKVDCTVGAPKVSYREAPTRETPFNYKHKKQTGGAGQYAHIVGKLI
                     PMDPQSAEPFLFENKVTGGRIPTEYIPSVEKGFRESLHKGPVAGYEVIGVQMRLEDGS
                     YHDVDSSTMAFEICARDCFRETFKKADPVLLEPIMKVEVEIPTEFQGPVTGAISSKRG
                     VILGTESRSGYSVIVAEVPLAEMFGYSNDLRSMTQGKGSFSMEFLKYQKLPSRFQEEI
                     VKKAQTLTKTATAKA"
     gene            complement(4908..>5203)
                     /locus_tag="VHS97_02990"
     CDS             complement(4908..>5203)
                     /locus_tag="VHS97_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447188.1"
                     /translation="GTDGDRSIARNRRDSLQPGQVTSLAPCYEIRNGTRFVVFLGSTA
                     TPVRNSTIELNTVDSPRTPSIVATPASGYFQISVKHGRNVPSSRIRSRIINVK"
BASE COUNT         1019 a         1496 c         1530 g         1158 t
ORIGIN      
        1 caactggtcg tagaccttgg gcatctcttc cttgagccga ctgatcggtt ccggggtccg
       61 gattccggca actacgtctt cgccttgggc gttgattaag tagtcgccgt agaactcgtc
      121 tgcgcctgtg ttctggtcgc gtgtaaaggc tacaccggtc cccgagtcgt tgccggtatt
      181 gccgaagacc atcgcctgca cattgaccgc ggttccgttc aaccccgtga ttctctcgat
      241 ccggcgatac tcgagggcct tgttccccat ccaactgttg aagaccgcca tgatcgcctt
      301 ccagagctga tctttcgggt cttggggaaa cgactccttg acgtgtttct ggtagacggc
      361 cttgtaacgg cgtaccaatt ccttgagatc gtcaccgtta agatcggtgt cgagcttgac
      421 gccgcgctcg ttcttgagac tttccagctc gtgctcgaac gcctcatgct cgactcccat
      481 cgccgtcgaa ccgaacatgt caattaagcg gcgatagccg tcgtaggcaa agcgggcatt
      541 ggcggtcttc ttggcgagac cttcgaccga ggcatcggtc agtccgaggt tcaagatggt
      601 gttcatcatg cccggcatcg acagcgccgc ccccgagcgg acactgacca ggagaggatc
      661 ggctggattg ccgaacttct tgccgacggc ctcttccatc cgcgataaag ccgactcgac
      721 ctgcggcctg acttcatcgg gaagctgacg gccacgttcg taatagtcgg cgcagacctc
      781 ggttgtgatg gtaaaccctg ggggaactgg aataccgatc acgctcatct cggcgagatt
      841 ggcccccttg cctcccaaca gatctttcat atcggagcgg ccctcggccg atccgccacc
      901 aaacgaatac acgtactttt gagtgttcgc catcgttctt cgttgctcca aatctcaggt
      961 gtgccaagcg cattcgaatg tcgaggtggg aaaagtgtgg tatgcgcgcg gaacagcctc
     1021 gcgatgcatt cgctcgtaat atcatggaat accgaacgtt atcgttttgg ccacccgtag
     1081 tcaagcgttc ggtcgcatga tgtcgacggc gcaccggcac ccgcacgggt ttggctggtg
     1141 tcgtttcgtc cgcggtcttg gcgggcggcg gattcactct ctcggcgtag cctgggaacg
     1201 gtcggcgccg accaagaggc ggcaatcctt actttgcgcc agccaggatt tccaactgac
     1261 gggtgcgggt cttgcgactc gcaccagccg cgctcccttg tgtttgccgg cggcggcctt
     1321 caagtgatca gcttcctcga gccagagact ctcggttccc cgatcataaa gcaacggctt
     1381 gccgtcgtgg aaataaccgc tggcggccat cgtcacgcgg tggccatcca gttctgactc
     1441 aaagatggaa taggcctctt ggttgggagc gaacaggttg gccacgatca ggtaaggatg
     1501 gtcttcgaca acgtcgttga tgacctgcac ctttcccaag acgatgacgg gataagcgca
     1561 ccgcagcccc accagtctgt ggccgacgac cgtacactcc tgtggaatcc gcttccagat
     1621 cgtacctccg ttgctttcga ccacgggatg atcgatggcc cgcgagatca ttgttgactc
     1681 tgggtgcctc aggtgagccg gatcgatgtc gcctcgtcgg aaatgaaacc agtgatgacc
     1741 gactccgttt tcccatcgcg accagaggag cgtttcatta ccctcttggc gaatcaaatc
     1801 cctgggaggc tgcgcgtacg agacggtggg cgcgatgtcg cgaaagccga cgagcgcgct
     1861 ggcttgggca cctccgaccg cgtcttttag cagggcccac tccagccata gactgcgagc
     1921 ccccacgaag acggatgccg ccatagcaag gcttgcagcg acgatcacac cgcgcttaag
     1981 ggccgttttc atcgacacac ggccagcgcc tggcagggcg gggctcacaa gaatctcgac
     2041 gttcgattcg aattgggaca tgcgttcgac cctcctgcat acttggtgcg gtgaaagcca
     2101 ctcaggcgat ggcaaggatg ggtacgaacg atccttatga cacctcggcg gacgtacgtc
     2161 aactccccag cggatattcg accaatcgga agaggcgcaa acattgcgcc cacagaaacc
     2221 cagggcgttg ccctgggctg ttctgttaat gcccctgcgg ggcaatgaag caaatatgcg
     2281 gcttcctgta tccatccccg aaggggctat cacatgccag cccagggcaa cgccctgggg
     2341 gcgctttcga caaccaaggt tttcaggcct tggccgtcgc tgttttggtg agtgtttgag
     2401 ccttcttgac gatttcttcc tggaaccgcg aaggcagttt ctggtatttg aggaattcca
     2461 tgctgaagct tcccttgccc tgagtcatgc tgcgcaagtc gttggagtaa ccaaacatct
     2521 cggccaaggg aacttccgcg acgatgacgg agtatcccga acgtgactct gtccccaaga
     2581 tcacgccgcg cttggaggat atggcgccgg tgacgggacc ttgaaactcg gtcgggatct
     2641 cgacctcgac cttcatgatc ggctcgagca ggacggggtc tgctttttta aaggtctcgc
     2701 ggaagcagtc gcgagcgcag atctcgaaag ccatggtcga cgaatcgaca tcgtgatacg
     2761 acccgtcctc gagcctcatt tgtacgccga tgacctcgta acctgcaacc gggcctttgt
     2821 gcaacgactc gcgaaagccc ttctcgacgg agggaatgta ttcagtgggg atacggccgc
     2881 cggtgacctt gttttcgaac agaaaaggct ccgccgactg cggatccatc gggataagct
     2941 tacccacgat gtgcgcgtac tggccggcgc cgccggtctg tttcttgtgt ttgtagttga
     3001 atggggtttc gcgggtcggc gcctctcggt agctcacctt gggagcgcca acggtacaat
     3061 cgaccttgta ttcgcggcgg atccgttcga cgtagatttc gaggtggagc tcgcccatgc
     3121 ccgagatgat cgtctcactc gtctcgtggt cgacgtgaac gcggaaggtg gggtcctcgc
     3181 gcatgaaacg gtgcagggct ttggcaagtt tgtcataatc ggcccgcttg aggggttgga
     3241 tcgacaggtc gatcacaggc tcggcggcat agatgctctc gagcgacagg tctgtcccct
     3301 cggcgcaata ggtgtcgccg gtcgcgcact cgattcccat cacggcgacg atgtcgcccg
     3361 cggaggccga gtcaatgtct tctttctggt cggcgtggac gcggagaatc cggctgatcc
     3421 tggcccgctt gcgctggcgg gaattgaaat agaacgtgcc cttggacaac gtaccctgat
     3481 agatgcgcac gaaggtgact tgcccgaatg gctcttcgac cagcttgaag gccatcgaca
     3541 ccagcggggc gtcgggatcg ggtgccaggg gaacttccgc tccgccgttg gtgtggtcct
     3601 tggcaaagat ctcgcgatcg agcgggctgg gcaagtagcg gcaaaccgcg tccaggagcg
     3661 attgaacccc cttgttgcgg tacgccgagc caaccatcac gggacagagt tgctgggcga
     3721 tcgttccttc gcggatcgtc ttgtggatga gatcgatcgg gacttcggct tcctcgagca
     3781 agagctccat gatctgatca gagatcatcg agagcgcttc gagcagacct tggcgggcgc
     3841 gttcggcggc ctcgaccatg tcggccggaa tcggctcgtt ccggatcgat tcgcctttgt
     3901 cgccgtcaaa atagacggca tggcgctcga tcaggtcgat cacccccagg aaactcgact
     3961 cggcgccaat cgggagctgg agcggaatgg cggtgagtcc cagcttggtc tcgagttgct
     4021 gaatcacgtt cgcggggttt gccccggtcc ggtccatctt gttgatgaat gcgatcctcg
     4081 ggactgcgta cctcttcatc tgacgatcga cggtgatcga ttgagactgc acgccggcga
     4141 cagcgcagag cacgagcaca gcaccgtcca ggacccgcag cgagcgctcg acttcgacgg
     4201 tgaagtccac gtggcccggc gtgtcgatga tgttaatttt ggtttcgttc cactggacgg
     4261 tggtcgccgc cgacgtgatc gtgatgcccc gctcgcgctc gagctccatg tggtccatca
     4321 cggccccttc acccttgact tctttgatga cgtgggtgcg gccggcgtaa tataagaccc
     4381 gctcggtcag cgtagtcttg cccgaatcga tgtgtgccga gattccaata ttccgtacgt
     4441 tgcgaagatt ttccatgatc caaaaacact caggcaccgt cgaaagggtg gaactccctt
     4501 tgttcgggct acgaactaca caccaagatt aatcagccgg atttggttca acccgtcgtt
     4561 ccaggacggg ctgaagggca tgccgaggcg agctacggag gcacaccaaa accgatctga
     4621 ggacaattct aacgcgtttt ggtgtggaac atgggaagag tccaggtaga gctaatgaac
     4681 acaagcgtaa tattttagca cgaccaccta ttcggtcaag gcccaatacc atccctgcac
     4741 cacaccttga tcaacttatt atttactata cgcacaaacg catgacttgc gtcgtatggg
     4801 ggatcgtgga ttccctcgaa cgacctcgtg cttgtaattg gatcttacga tgttgctgca
     4861 tgacatgtgc ggtgatgagc ggctgaaaga cgattttccg agagttctca cttaacattg
     4921 ataattcggc tgcgaatacg tgacgagggg acattccgtc cgtgcttgac gctaatttgg
     4981 aagtagccac ttgctggcgt ggctacaatt gagggtgtac gtggcgagtc caccgtgttg
     5041 agctcgatcg tcgagtttct aaccggcgtt gcggtgctgc caagaaaaac aacgaagcgc
     5101 gttccgttcc tgatttcgta gcaaggagcc agagatgtta cttgccccgg ctggaggctg
     5161 tcgcggcgat ttcgcgcgat cgatcgatcg ccgtccgttc cag
//
LOCUS       DATAQL010000181         4666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_781841, whole genome shotgun sequence.
ACCESSION   DATAQL010000181 DATAQL010000000
VERSION     DATAQL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781841"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3038)
                     /locus_tag="VHS97_02995"
     CDS             complement(<1..3038)
                     /locus_tag="VHS97_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447189.1"
                     /translation="MSRVSLEPEDSPLDPALPSAPALAAAPDLLPDGPTDRSATEGHA
                     AGEIENAQAVAEPARDGWALIALVPYDGREPPPTVTDELANGVWCAVSALWHPSLLAR
                     AAGLPRIEPVDSPSPPGAREIRVIATGAGDQLPSGYRTQAEDARTALLDSGTDRADLI
                     AQIQARVGATGALEMIENEGMTTSAQVFLALGTVRFMLRELTVAMGHADALDHDSLTR
                     ELLAGAHHWQIGDWASAVNRLRAGFEVMTQARERFYPVDAYLLDLCLLDPAVPAGSIA
                     ELLGNPIAISFIAQARAIENLALHDPQRLAALRQAIADGWADVAGGSYSEEEDPLLPL
                     ESILWQFRRANDVYRTHLDDRNAETFARRRFGLFTQLPQFAKRFGFHFALHLALDAGR
                     FPVPPETKRLWESPDGSSLETLFRPPLAADRPAQGWFVPWRMAATMKNDHVAALPMVH
                     WPQPVAPWYLDLRRAASYSPVLGRWTTLNDFFHLTDRPYETFRPEPDHYRTPYLAQAV
                     SRREPEPIARLARHHRLKARAQAAGTTVALAHAIALSTPTATTEPGESPESGILEEID
                     TLIETRRHAEAGAAIDRVLPDWAAELARRIVAVGDATATERQARARPGYLVLNPIHVP
                     RRAAVVLPDAALDLRPEGPLRVAQFTEEGVLGVVDLPALGFAWVPKEASLDRPAAEAG
                     GLSAKGRQLKNESIAIEIDAATGGIRSLASAGESTARVGQQLVMTGLADAQGKPVTSL
                     MRSDRFEIEYGGPALVQATSSGGLVDPQSGNRLASFVQRYRLWAGRPILEIEITLSDL
                     DSAWLSRAAQADPYAVYLACRWAWPDPNSMLRRTVFWSSEITESERPETPDAFDISTR
                     TQRTALLFGGLPYHRKVGSRMLDTLLVAGSESTRTFTLGVVLDLEHPFHAAQDLIAPP
                     LVVPIDDGPPSLGSSGWLARIDRKGVAVSHVGFASATGDDRGWGLVFHLLETAGQAGR
                     CRLRLFRNPLGARQADFLGETIVDLSIDGDAVLVDLTPHEL"
     gene            complement(3043..3783)
                     /locus_tag="VHS97_03000"
     CDS             complement(3043..3783)
                     /locus_tag="VHS97_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEX3447190.1"
                     /translation="MSTSAADSAPSWVPLAPRERRVLGVLAEKAKTTPEYYPLSLAAI
                     VTGCNQKSNRDPVTNYDQDDVEETLHSLRKKGAVILVEGSGRVQRWKHTLYDWLKVSK
                     VELAVMAELLLRGPQTEGELRARANRMEPLADLPALQAILEALAPRGLVKHLSPPGQK
                     RGVYVAHGLYPADEFEKVRAAFANRFAGEDEAPARLSTARVDQAAAPVAPAWTAELTA
                     LRSEVEQLRENLEALTNEVRDLKSALGV"
     gene            complement(3942..4460)
                     /locus_tag="VHS97_03005"
     CDS             complement(3942..4460)
                     /locus_tag="VHS97_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3447191.1"
                     /translation="MALVSYVSNQDAAEKIKPVFEGMEKKIGVVPNVFRAMAHNPEML
                     EAFLALNSTLAKTKLDGKLRELAYIKTSELNGCDYCLHHHRALGKKAGLNDRQVAETA
                     HFETSDAYDDIEREVMLYAEEVTRHINVDDALVDRIKQKLGDRELVELAMTVGIANFT
                     NRVTETLRLELP"
BASE COUNT          810 a         1504 c         1509 g          843 t
ORIGIN      
        1 agctcgtgcg gggtcagatc gacgagcacc gcgtcgccgt cgatcgacag gtccacgatc
       61 gtctcgccca ggaaatcggc ctgacgcgct ccgagcggat tgcggaacaa tcgcaagcgg
      121 cagcgccctg cctggccggc cgtctcgagc aagtgaaaaa cgaggcccca gccgcggtcg
      181 tcgccggtcg cactcgcgaa ccccacgtgc gagacggcaa cccccttgcg gtcaatccgc
      241 gccagccacc cgctggagcc gagcgacggg gggccatcgt cgatcgggac gaccagaggg
      301 ggtgcgatca gatcctgcgc cgcgtggaat ggatgctcga ggtcgagcac cacccccagc
      361 gtgaacgtcc gggtggattc ggaaccggca accaagagtg tgtcgagcat gcggctgccg
      421 accttgcggt ggtacggcaa gcccccaaac aaaagcgccg tccgttgagt ccgtgtcgag
      481 atgtcgaacg cgtcgggggt ctcggggcgc tccgactctg tgatttcgct cgaccagaag
      541 accgtccggc ggagcatcga attggggtcg ggccaggccc agcggcaggc gagatagacc
      601 gcgtaagggt cggcctgggc ggctcgagaa agccatgccg aatcgagatc actcagcgtg
      661 atctcaattt cgaggatcgg ccggcccgcc cagagccggt accgctgcac aaacgaagca
      721 agccgattgc cgctttgtgg atcaaccagc ccgccgctcg atgtcgcctg cacgagtgcg
      781 ggaccgccgt attcaatttc gaatcggtcg gaccgcatca gcgatgtcac gggtttgccc
      841 tgcgcatcgg ccaacccggt catgacaagt tgttgaccga cccgagccgt cgattcgccc
      901 gcggacgcca ggctgcggat tcccccggtg gcggcgtcga tctcgattgc gatggattcg
      961 tttttgagct ggcggccctt ggccgacaat ccccccgcct cggctgccgg acggtccagg
     1021 ctggcctcct tgggaaccca tgcgaaaccg agcgcgggca gatccacaac gcccaacacc
     1081 ccttcctcgg tgaactgggc gacccgaagt ggaccctccg gtcgcaggtc gagcgccgcg
     1141 tcgggcaaga cgaccgccgc gcgccgcggc acatgaatcg ggtttagaac gaggtatccg
     1201 gggcgggctc gcgcctgccg ttccgtggcc gtggcatctc ccactgcgac gatccgccgc
     1261 gccagttccg ccgcccagtc cggcaaaacg cggtcgatcg ccgctccggc ctcggcgtga
     1321 cggcgggttt cgatcagggt gtcaatttcc tcgagtatgc cgctctcggg cgattcgccg
     1381 ggctcggtgg tggctgtagg ggtggaaagc gcgatcgcgt gggcgagggc cacggtcgtc
     1441 ccggccgctt gggcgcgcgc cttcaaacga tggtgccgcg ccaaccgcgc gatcggctcc
     1501 ggctctcgcc ggctgaccgc ctgcgccaga tagggcgtac ggtagtgatc gggctccggc
     1561 cgaaaagtct cgtacggacg gtccgtcaga tggaaaaagt cgttgagcgt tgtccatcgg
     1621 ccgagaaccg gcgaatagct ggcagcccgg cgcaggtcga gataccacgg cgctaccggc
     1681 tgaggccagt ggaccatggg gagcgcggcg acatggtcgt tcttcatcgt cgcggccatc
     1741 ctccagggca cgaaccatcc ctgtgccggc cgatcggccg cgagcggcgg ccggaacaga
     1801 gtctctagac tgctgccatc ggggctctcc cacaaccgct tggtctcagg aggaaccgga
     1861 aaccggcccg catcgagcgc cagatggagc gcgaaatgaa atccaaaccg tttggcgaat
     1921 tgtggaagct gggtgaagag gccaaatcgc cggcgcgcaa atgtctcggc attgcgatcg
     1981 tcgagatgcg tgcggtagac gtcgtttgcc cgccggaact gccacaagat cgattccagt
     2041 ggtaagagcg ggtcttcctc ctccgagtaa ctaccgccgg cgacgtccgc ccagccatcg
     2101 gcgatcgcct gtcgcagcgc agccagccgt tgcggatcgt gcaaggccag gttttcgatc
     2161 gcccgcgcct gggcgatgaa cgagatggcg atcggatttc ccagcaactc ggcaatcgat
     2221 ccggcgggga cggccggatc gagcaggcag agatcgagca aataggcatc gaccgggtaa
     2281 aatcgctcgc gcgcctgggt catcacctcg aagccagctc ggagccgatt gactgcgctc
     2341 gcccagtcgc cgatctgcca gtggtgggcc cctgccaaga gctcgcgcgt caggctatcg
     2401 tggtcgagcg cgtcggcatg tcccatcgca acagttagct cgcgaagcat aaagcgcacg
     2461 gtgccgagag ccagaaacac ttgtgccgac gtcgtcatgc cctcattttc gatcatttcc
     2521 agggcaccgg ttgcgccgac acgtgcttga atctgggcga tcaagtccgc gcggtcggtg
     2581 cccgagtcga ggagggcagt ccgggcgtcc tcggcctggg tgcggtagcc ggatgggagc
     2641 tggtcacccg ctccagtcgc gatcactctg atctcccgcg cgccgggcgg cgacggggaa
     2701 tcgaccggtt cgatccgggg caagccggca gcgcgcgcca ggagcgaggg atgccaaagt
     2761 gccgagaccg cgcaccaaac tccgtttgcg agttcgtcgg tcaccgttgg ggggggctcg
     2821 cgtccgtcgt agggaaccag cgcgatcagc gcccagccgt cccgagccgg ctctgcaacc
     2881 gcctgagcgt tttcgatttc gcccgccgcg tggccttccg tggctgaccg atcagtcggc
     2941 ccatcaggca gcaaatcggg ggcggccgcc aacgcgggag ccgacggcag cgccggatca
     3001 agcggtgaat cctccggctc gagagaaact ctgctcattc ggtcaaactc cgagcgcgga
     3061 cttgaggtcg cgcacttcgt tagtgagggc ttccagattt tcgcgcagtt gctcgacctc
     3121 ggagcggagc gcggtgagct cggcggtcca cgccggtgcg acgggcgcgg ccgcctgatc
     3181 gacgcgcgcg gtcgacaacc gcgctggtgc ctcgtcttcg ccggcgaacc tgttggcaaa
     3241 tgccgcgcgg actttctcga actcatcggc cggatagagg ccgtgagcca catacacgcc
     3301 gcgcttctgt cccggcggcg agagatgttt aaccaagccc cgcggcgcca gtgcctcgag
     3361 gatcgcctgc agggccggca ggtcggccag tggctccatc cggttggcgc gggcgcgcag
     3421 ctcaccttcg gtctgtggtc cgcgcaaaag tagctcagcc atcacggcca gctcgacctt
     3481 gctcaccttg agccaatcgt aaagagtgtg cttccagcgc tggactcggc cgctcccctc
     3541 gaccaagatc acggcccctt tctttcgcag actatgcagt gtctcctcca cgtcatcttg
     3601 gtcgtagttg gtgaccgggt cgcgattgga tttctggttg cagccggtca cgatcgccgc
     3661 cagcgacaac ggatagtatt ccggagtagt cttggccttt tcggccaaga ctcccagcac
     3721 ccgccgctct cgcggcgcca agggaaccca cgacggtgcc gaatcagccg cgcttgtact
     3781 catgttgcat tggttcctga ggacgttcgc gtgagagaaa tcacagggcg atcagtaggc
     3841 ggttttgttg cgccacatgc ccacggcaac tttcgggctg tcacgtttca agccgcgtac
     3901 gcggcggcat cataaagccc ggggtgtaaa ccccgggtgt actacggcag ctcgagccgc
     3961 aatgtctcgg tgacccgatt ggtgaaattg gcgatgccga cggtcatggc cagctcgacg
     4021 agctcacgat ccccgagctt ctgcttgatc cgatccacga gagcatcgtc gacattgatg
     4081 tgccgggtca cctcttctgc atagagcatc acctcccgct cgatgtcatc gtacgcgtca
     4141 cttgtctcga aatgagcggt ctcggcgacc tgtcggtcat tgagccccgc tttcttgccc
     4201 agtgcccgat ggtggtgcaa gcagtagtcg cagccattca actccgacgt tttgatgtac
     4261 gccaactcgc gcagcttgcc gtcgagcttg gttttggcca aggtagagtt gagcgcgaga
     4321 aacgcctcga gcatttcggg gttgtgggcc atcgcccgaa acacgttggg cacgacgccg
     4381 atctttttct ccatcccctc gaagaccggc ttgatctttt cggccgcgtc ttggttggac
     4441 acataactga caagcgccat ggtgaagtct cctggtcggc tggattccca gagggttgtc
     4501 ttttggcacg gatttgttgt tgaaacgtcg cgtcctgttc gcgctcatcg tagccgcggc
     4561 tgcgcagccc cttcaaggtc tcactcgatg ttgcacgact cgcgtgggtc gtcgaacggc
     4621 gagctcctcg ctgatcccgg tctatagagt gcctgacgaa ccgcgg
//
LOCUS       DATAQL010000182         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3836849, whole genome shotgun sequence.
ACCESSION   DATAQL010000182 DATAQL010000000
VERSION     DATAQL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836849"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VHS97_03010"
     CDS             <1..1429
                     /locus_tag="VHS97_03010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3447192.1"
                     /translation="RLGPIPRFVPPSFERRSLSSGLELLIVERHELPILTVDLIVKSG
                     ETSTPKGKEGLGSIAASLLDEGTQTRNALEVAGELAEIGATLAAAGELESTTVSLTTL
                     TRHLDRGLDLYADIIMNPSFPEKELSRLKLQRLAQLKARADDPEQTAAAVFPRLIYGM
                     DHPYGRPDVGTARSVESITRDDAVGFYKRIMVPGNAALVVVGDVRPDTITAALEARLR
                     KWSPGPVPPAPSVLSPATRQRRGSYLIDKPAAAQSVVTVGKIAAARKSPDYFALRVMN
                     AILGGQFASRLNMNLREDKGYSYGAQSSFTFFKGPGPFEAEATVQTAVTKESLIEIMK
                     ELTDITGRRPVTEAELSFAKQGLIQGFPNRFETTFGVAGQIANLVIDDLPDDEFTHYQ
                     ARLEAVTNAEVDRVAREYVTPASMTILVVGDRSQIEGPLKSLPFVETIQRLDTEGNPL
                     APPASAKPAAAAKTGPATGSRATAN"
     gene            complement(1460..>1921)
                     /locus_tag="VHS97_03015"
     CDS             complement(1460..>1921)
                     /locus_tag="VHS97_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3447193.1"
                     /translation="AVTGWRVGTIIAPPQLTHAFRQVHDFVSIGAAAPLQEAGAVAYR
                     LPRTYYAQLAADYQARRDRFCKALWDIGFDFDPPHGAYYIMAGIGAFAATDDIEFARY
                     LVRDIGVATVPGSSFFQDKALGRPFVRFCFCKRDETLDEAAGRLQALRVMV"
BASE COUNT          383 a          616 c          601 g          321 t
ORIGIN      
        1 caggcttggg cccataccgc ggtttgtgcc gccaagtttc gagcgccgtt cgctttcaag
       61 cggtctcgag cttttgatcg tcgagcgcca cgagctgcca atcctgaccg tcgacctgat
      121 cgtcaagtcc ggcgagacct ccacgcccaa gggcaaggaa ggtttgggct cgatcgccgc
      181 cagcctgctc gacgagggca cccagacacg caatgctctg gaggtggccg gcgagctcgc
      241 cgaaatcggc gccacccttg ccgctgcggg cgagcttgaa tcgaccaccg tcagcctgac
      301 gaccttgaca cggcacctcg accgcgggct tgatctctat gccgacatca tcatgaaccc
      361 atcctttccc gaaaaagagc tgagccggct caagctgcag cggctggctc agctcaaggc
      421 ccgcgccgac gatccagagc agaccgccgc cgcagttttc cctcgcttga tttacgggat
      481 ggaccatccc tatggccgac ctgacgtggg gacggcccgc tcggtcgagt cgatcacgcg
      541 cgacgatgcc gtagggtttt acaagcggat catggtgcca ggcaacgcgg cgctcgtggt
      601 cgtcggcgac gtccggccgg acacaatcac ggccgctctc gaagcacggt tgcggaagtg
      661 gtcgccgggc cctgtgcctc cggctccgtc ggtcttgtct ccggccacac gacagcgccg
      721 ggggtcctac ctgatcgaca aaccggctgc cgcgcaatcg gtcgtaaccg tgggcaagat
      781 cgcggcggca cgcaaatcgc ccgactactt cgccctgagg gtaatgaacg cgatcctcgg
      841 gggccagttt gccagtcgcc tcaacatgaa cctccgtgaa gacaagggtt acagttacgg
      901 tgcgcagtcg agctttacgt ttttcaaagg ccctggcccg ttcgaggcgg aagctactgt
      961 gcagaccgcc gtcaccaagg aatcgctcat cgaaatcatg aaggaactga ccgacatcac
     1021 cggccggcgt cccgttaccg aggccgagct atcgtttgca aagcaggggc tcatccaggg
     1081 tttccccaat cgattcgaga ccacgttcgg ggtcgccggc cagatcgcta acctggtgat
     1141 cgatgacctg cccgacgatg agttcaccca ttatcaggca cgactcgaag ccgtaaccaa
     1201 cgcggaagtc gaccgggtcg cgcgcgagta cgtcacgccg gcgagcatga caattctggt
     1261 cgtgggagac cggtctcaga tcgaagggcc gctcaaaagc ctgccgttcg tcgagaccat
     1321 ccagcggctc gacacggaag gtaaccccct ggctcctcca gccagcgcga agccggccgc
     1381 ggccgcgaaa acaggcccag caaccggatc gcgggcgact gcaaactgag cattgaggat
     1441 ttgagaggtt ttgaaagctc taaaccatca cgcgcaaggc ctgcagccgc ccagcggctt
     1501 catcaagcgt ttcgtcacgt ttacagaagc agaagcgaac gaacgggcgt cccagggctt
     1561 tatcttgaaa gaagctcgag ccgggcacgg tggcgacgcc gatatcgcgg accaggtagc
     1621 gggcgaactc aatatcgtcg gtggccgcga aggccccgat gccggccatg atatagtagg
     1681 ctccgtgggg cgggtcgaaa tcgaaaccga tgtcccaaag ggccttgcag aagcggtcgc
     1741 gccgcgcctg gtagtcggcg gcgagctgcg cgtagtaggt ccgaggcagg cgatacgcga
     1801 cggcgccggc ctcttgcaag ggagcggccg cgccgatcga aacgaagtcg tgtacctggc
     1861 gaaacgcatg cgtcagttgc ggcggcgcga tgatcgtgcc gacccgccag ccggtcacgg
     1921 c
//
LOCUS       DATAQL010000183         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5210447, whole genome shotgun sequence.
ACCESSION   DATAQL010000183 DATAQL010000000
VERSION     DATAQL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5210447"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1152)
                     /locus_tag="VHS97_03020"
     CDS             complement(112..1152)
                     /locus_tag="VHS97_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447194.1"
                     /translation="MVGSKHVRLRVGVIGLGRLWETRHKPSLARLDDRFRVTAVYDPV
                     FRRAELEAAQIGCAACEGLSALVERPDVDVIYLLSRQWFGLHPIALACAAGKPVYCAL
                     PLADELTELESLAKQIEQSGIVFMPEFARRCYPATLRLKELLATELGPPRLVVGHTRL
                     YGFDRYAIPGPTTQIAPAPLTVDPGSYLIDWCCFVFQSHPEGVQGFRCHVLPSPDHVD
                     QESDFESFVAAFPGGATAQMSYGRYHRVPWGDASRFLPAPGFQVFAERGAAWLEMPEK
                     IQWSSSAGTKEERLALEPTVGDVLNDQFHRLVRGDHSLAPTIRDALEIARLANDLRRS
                     QRGRAGSARPAT"
     gene            1327..1776
                     /gene="dtd"
                     /locus_tag="VHS97_03025"
     CDS             1327..1776
                     /gene="dtd"
                     /locus_tag="VHS97_03025"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX3447195.1"
                     /translation="MRAVLQRVARAAVEVDGTCVGRIERGWLVLLGVAQGDTEDDAGW
                     MAEKVLQLRGFEDDAGKMNRSVADVGGSVLVVSQFTLLGDCRGGRRPSFTAAADPAVA
                     ERLYLLCAETLRRSGLDVATGVFRAMMQVELVNDGPVTFVLDSRKSL"
     gene            1838..3379
                     /locus_tag="VHS97_03030"
     CDS             1838..3379
                     /locus_tag="VHS97_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX3447196.1"
                     /translation="MQPATGRPPLLLGIDVGTQSLRAALVDAFGRTVSFGIAPFDTTY
                     PRPTWAEQEPLQWWSAARAAIGLALARGDVDPGQVVGIGLDCTACTVVACDSGGQPLR
                     KALLWMDQRAFHEAGLISETGDPILRYVSGRVSPEWMLPKALWLKRHEPEVYARAERI
                     VECTDWMMYKLTGEWTLSLNHIAVKWNYARPDGGWPVGLMKAVELDDLLTKWPSRIVP
                     LGKGESPLGEQAAREIGLPAGIPVAQGGIDAYLGMLGLGATSDGDVAVIVGSSTCHLA
                     QSRNGVFGSGAAGCYPDATVLGLYTLEAGQTATGSILDWYRRHFAGFQQHEALERGIN
                     VFQVLDEQAAAVPPGAEGLVVRDDWQGNRSPYKNPQARGAIAGFSLAHGPGHVFRAIY
                     EATACGTRHILEDAKAHGLNVERIFVGGGGARSALWLQIHADILKQPVHLARESEACA
                     LGSAMAASVAAGIYPDFDAAARAMVAIERLVEPFSANAGVYDELFGRYVELYQRLNQL
                     PSSSH"
BASE COUNT          665 a         1090 c         1161 g          649 t
ORIGIN      
        1 gccgaccacg agagcggcgg cggtgagcaa accccaaacc gagaagccgg cagtggtcag
       61 gccggcacga gctaaacgat gttcaggatc catcctcatg actgtcagtc ctcacgtcgc
      121 cgggcgagcg ctgccggccc gcccgcgctg agagcggcgc aggtcgttgg ccaaacgggc
      181 gatctcaagg gcatcgcgga tcgtgggcgc cagcgagtga tcgccccgga caaggcggtg
      241 aaactggtcg ttgagcacgt cgccgaccgt gggctcgagc gccagccgtt cttcctttgt
      301 cccggccgag ctcgaccatt ggatcttttc cggcatctcc agccaggctg cgccgcgttc
      361 ggcaaagacc tgaaagcccg gagcgggcaa gaagcgactg gcgtcgcccc agggaacgcg
      421 gtggtagcgg ccatacgaca tctgggccgt cgcgccgccg ggaaaagctg ccacgaagct
      481 ctcgaaatcc gattcctggt caacgtgatc gggcgaaggg agcacgtggc atcgaaagcc
      541 ctgtacaccc tcggggtgcg actgaaagac gaaacagcac caatcgatca gatagctgcc
      601 aggatccacc gtcaaaggcg ccggagcgat ctgcgttgtc ggccccggga tcgcgtagcg
      661 gtcgaaacca tagagtcgag tatgtccaac gaccagtcgc ggtggaccga gttccgttgc
      721 cagcagttcc ttgagccgca acgtcgccgg gtaacagcgc cgggcaaatt cgggcatgaa
      781 gacaatgccg ctctgctcaa tctgtttggc cagcgattcc agctcggtca gttcgtcggc
      841 cagcggcagt gcacagtaca ccggttttcc cgcggcgcat gccagggcaa tcggatgaag
      901 cccgaaccat tggcgcgaca agaggtaaat cacgtcgacg tcgggccgtt ccacaagtgc
      961 cgacaatcct tcgcaggccg cgcagccgat ctgggcggcc tcgagctcgg cccggcgaaa
     1021 gacagggtca taaaccgctg tcacccggaa ccgatcgtcc agtcgggcca aggacggctt
     1081 gtgccgggtt tcccagagcc ggcccaaccc tatgactccc acacggagcc gcacgtgctt
     1141 cgacccgacc aagcccgcct cctcgcgacg cgatccgtgc tcgccgtgac gctcgctgta
     1201 actgtcgcag tgagaaggtt atcagcttac ggcatcctgg gctacccaaa ttcacaggca
     1261 agcaacacga ctctgtccgc gttattatga ccctatagtt gccggcgacc gtgcggagtt
     1321 tgatccatgc gtgcagtgtt gcagagagtg gccagggctg cggtcgaggt cgacgggaca
     1381 tgtgtcggcc gtatcgagcg cggctggctg gtattgctcg gtgtcgccca gggtgatacg
     1441 gaggacgatg ccggctggat ggcggagaag gtacttcaat tgcgcggttt cgaggacgac
     1501 gcgggcaaga tgaaccgaag tgtcgcggac gttggcggca gcgtcttggt ggtcagccag
     1561 tttactcttc tgggcgactg ccgcggtggc cggcggccga gcttcaccgc ggcggccgat
     1621 ccagcggttg ccgagaggtt gtacctgctg tgcgcggaga ccctgcgacg gtcggggctg
     1681 gatgtcgcca ccggcgtctt ccgcgccatg atgcaggtcg agctggtcaa cgacgggcca
     1741 gtgacgtttg tgttagacag tcgaaagtcg ctttgaacag attcgaatga cttaggtcga
     1801 gacaggtaat tcaagcagag ccgagaggcc agtttccatg cagccagcga cgggtcggcc
     1861 acctttactc cttggaattg atgtcggcac ccagagcctg cgtgccgccc ttgtcgacgc
     1921 gtttggacgg acggtttcct tcgggattgc gccctttgat acgacgtatc cgcgaccgac
     1981 ctgggccgag caagagccgc tccaatggtg gtcggcggca agagccgcga tcgggctggc
     2041 gctggcacga ggagacgtgg accctggaca ggtcgtcggc atcggtcttg actgtacggc
     2101 ctgcaccgtc gttgcctgtg actcgggcgg tcaacccctg cgcaaggcct tgctctggat
     2161 ggatcagcgt gccttccacg aggccggact tatcagtgaa accggagacc cgatcctgcg
     2221 ctatgtctcc ggccgtgttt ctccagagtg gatgcttccc aaggcactct ggctcaagcg
     2281 ccatgagccg gaggtctacg cccgagcgga gcggatcgtc gagtgcaccg actggatgat
     2341 gtataaactg actggcgaat ggacgctctc gctcaatcac atcgcagtca agtggaacta
     2401 tgcccggcca gatggcggat ggccggttgg actcatgaag gccgtcgagc tcgacgactt
     2461 gctgacgaaa tggccttctc gaatcgtgcc gctgggcaag ggagagtcgc ccctgggcga
     2521 gcaggcggca cgtgaaatcg gtttgcccgc ggggattcct gttgctcagg gaggaatcga
     2581 cgcatacctg gggatgctcg gactgggggc gacgtcggac ggcgatgtgg ccgtgatcgt
     2641 cggctcgagc acctgccacc tggctcagtc gcgcaatgga gtctttggct cgggcgcggc
     2701 cggctgttat ccggacgcga ccgttctggg tctctacact ctggaggccg gccagaccgc
     2761 caccggttcg atactcgact ggtatcgacg ccatttcgcc ggcttccaac agcacgaggc
     2821 cctagagaga ggcatcaacg tgttccaggt gctcgacgag caagccgcgg ccgtgccgcc
     2881 aggcgcggag ggtctggtgg tgcgcgacga ttggcagggg aatcgctctc cgtacaaaaa
     2941 cccacaagcc cgcggcgcga tcgcgggttt ctcgcttgcc cacggccccg gccacgtgtt
     3001 ccgggctatt tacgaagcga ccgcttgcgg cacgcgacac atcctcgaag acgcaaaagc
     3061 tcatggcctc aacgttgaac ggatctttgt cggaggcggc ggggccaggt cggctctctg
     3121 gctccagatc cacgccgaca tcctcaagca gccggtgcac ctcgcacgcg agagcgaggc
     3181 gtgtgcgcta ggctcggcca tggccgcctc cgtggcggcc ggaatttatc ccgatttcga
     3241 tgcggccgcc cgtgccatgg tcgccatcga acgactcgtg gaacccttca gtgccaatgc
     3301 gggcgtttac gatgagcttt tcgggcgtta cgtcgagctc tatcagcggc tgaatcagtt
     3361 gccatcgtcg tcgcattgac ggccaagcag agacgcacga gaatcgccga tgaccagccc
     3421 cccgcacgcg ttgtgaggtg acgacgtgac gagccaggat agccgtcaag ctgcaaatgc
     3481 gttcattgtc acgttctatt ttgatcaagc cacgtcgctg ctcaaccgcg ggctctttgc
     3541 cgagtccgag acgtacttcc gagag
//
LOCUS       DATAQL010000184         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2462522, whole genome shotgun sequence.
ACCESSION   DATAQL010000184 DATAQL010000000
VERSION     DATAQL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462522"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1232)
                     /locus_tag="VHS97_03035"
     CDS             complement(84..1232)
                     /locus_tag="VHS97_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX3447197.1"
                     /translation="MPTRWVNVESIGSYFESLSDPRHTRNRKHLLVDIVVIAVCGMVC
                     GCDGPTAIHRWAENRRDWLAQSLSLPNGIPSRDCIRRLLIALKPRAFQECFQDWIAGA
                     IRTDDCDPDRLIAIDGKTCRGSHDASRGLGPLHIVSAWASEEGIALGQVATEEKSNEI
                     TAIPELLKQIDLTGSLITIDAMGCQKEIARDIVDGGGDFVISLKDNQPKLRESIEAHF
                     EKHLERDLEDLRYRAYETSESAHGRIDERSYVLTKIPPDFELKEEWPWIKAIGYSLRI
                     TQHADGSETDETRYYILSRYLSGKRFSEAVRGHWGIESMHWVLDVNFREDDSRARERA
                     LGNNLSWLRRFAVSLLKRHPVKDSLRGKMLRCMMNTDFLTEVLTIQGD"
     gene            1315..1542
                     /locus_tag="VHS97_03040"
     CDS             1315..1542
                     /locus_tag="VHS97_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447198.1"
                     /translation="MYQSAPDSASPYRLPIYARWPCAAGRAYAAYGGVYILSSLAWLW
                     VVEGTRPDRWDVIGAAVCLIGAAVILYGPRS"
BASE COUNT          272 a          544 c          491 g          316 t
ORIGIN      
        1 ggagtacaac gccttcatcc gccccgacgg cacttgggtc cgccagtacc gcttccccgg
       61 cccccgtcca cggccagcgc atatcaatct ccttgaattg tcaggacttc cgtgaggaag
      121 tccgtgttca tcatgcaccg aagcatcttg cctcggaggc tgtctttgac cggatgtcgc
      181 ttgagcaggc tgacggcgaa tcgcctcagc cagctcaaat tgttgccgag cgctcgctcg
      241 cgcgcccggc tgtcgtcctc gcgaaagttc acatccaaca cccagtgcat cgactcgatg
      301 ccccaatgac cgcggaccgc ctcactgaat cgtttgccac tgagataacg gctgagaata
      361 tagtaccgcg tctcgtccgt ctcgctgccg tccgcatgct gtgtgatccg cagcgagtag
      421 ccgatcgcct tgatccacgg ccattcctcc ttcagctcga agtcgggcgg gatcttggta
      481 aggacatacg accgctcatc gatccgaccg tgagccgact cgctcgtctc gtaggctcga
      541 tacctgagat cctccaggtc gcgctcgaga tgcttctcga agtgggcttc gatcgactct
      601 cggagcttcg gctggttatc cttgaggctg atcacgaaat cgcctccgcc gtcgacgatg
      661 tcgcgcgcga tctctttctg gcagcccatg gcatcgatcg tgatcaagga gcccgtcaga
      721 tcgatctgct tcaggagctc ggggatcgcc gtgatctcgt tggatttctc ctcggtcgcg
      781 acctggccca gggcgatccc ttcctcgctg gcccaggcgc tgacgatgtg cagcggaccg
      841 agtcctcgcg acgcgtcgtg cgacccccga cacgtcttac catcgatggc gatcagccga
      901 tccggatcgc agtcgtcggt ccggatcgcg ccggcgatcc agtcctggaa gcactcctga
      961 aacgcccggg gcttgagcgc gatgagcagt cgtcggatgc aatcgcgcga ggggatgccg
     1021 ttggggagcg aaagggactg ggccaaccaa tcccggcgat tctcggccca gcgatggatg
     1081 gccgtaggac catcgcagcc gcacaccatc ccgcagaccg cgatcaccac gatgtccacc
     1141 agcagatgct tgcgatttcg cgtatgccgc gggtcggaga gcgactcgaa gtaggaaccg
     1201 atcgactcga cgttcaccca tctggttggc acgtgacgca tcctggccgg aggccagcgg
     1261 gtcccacggg caatccgtga tcgccgcacc gcgcgagcac cgcctcaagc caaggtgtac
     1321 cagagcgcgc ccgacagcgc cagcccatac agattaccca tttatgcgcg ctggccgtgc
     1381 gccgcgggtc gggcctacgc cgcctatggc ggggtctata tcctgtcgtc gctggcctgg
     1441 ctctgggtcg tggaagggac gcggccggac cgctgggacg tgatcggtgc cgccgtctgc
     1501 ctgatcggtg ccgccgtcat cctttacggc ccgcggtcct gatgaaggca gttccggctc
     1561 gcgtcgggtc ttgcccggcc gccgggatgg cggaccctga gcgtcagccg ccgtataatg
     1621 ttc
//
LOCUS       DATAQL010000185         5899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1700161, whole genome shotgun sequence.
ACCESSION   DATAQL010000185 DATAQL010000000
VERSION     DATAQL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700161"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2416
                     /locus_tag="VHS97_03045"
     CDS             <1..2416
                     /locus_tag="VHS97_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3447199.1"
                     /translation="VNGFELGLDGWVYGANGDSGGTVRSVKTRKTIEISGRDFRFRPD
                     TGDFEAESGRTQYGRHRDDWGHWFGNNNPNWGWHYVLANRDLSRNPDFAPPDTRVTLE
                     PDTRLFPISRTLARFNDPGAANHVTSANSPTPYRDELFGAEFATSLFVSEPVHNLVHR
                     MVLEPQGMSFRGHRGPNDANREFLASSDNWFRPAMLKTGPDGALWIADMYRAVIEHPE
                     WIPDDWERRLDLRAGSEQGRIYRVYPLDKKLRPIPQFDKLDTTQLVAAMESPSGWQRD
                     TVQRLLLHRRDPAAIAPLRALAIATKQPKTRVQAIWTLADLNGLDEPCALAGLDDPDP
                     RVRESVIAAVSSRAGQSRIIMDALIKTAEDANAHVRLQAALALGDCADRRAGEALAAL
                     ARRDGNDQWLRAAILCSAVPHVDTLLVALLRDTAPGVQKPESFEAMVGPLLSVAVSHS
                     DRAPIELLTRSIAVPSGQGGRYAPWQFSTLAGLLEARDHPNRPFKLDLDKPFASVWAA
                     AHRVVSDESAPEAERVGAVRLIGYRARRDLKNRDLLIGLLRPQVSAGLQQAAVAALGR
                     TDDPKLADLLLVDWKRYSPSIRDSILDVVLSRTAWTSSLLSCLEDGCVPPPEIDPARR
                     QQLLTRKSDRLRSRAEAVFAHQSQPRQAVVDAYRASLAMRGDKSAGATVFKKVCASCN
                     RLGNEGVEVGPDLAALNDKSPEALLIAILDPSRAFEAKFASFSIATVDGRVMNGLIAA
                     ESATAVTLRRQDGKEDVLLRSQIEEMTGSSQSLMPEGLEKDLKPQDLADLITFVVSAR
                     PSLKPE"
     gene            2423..3826
                     /locus_tag="VHS97_03050"
     CDS             2423..3826
                     /locus_tag="VHS97_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3447200.1"
                     /translation="MPLPTDIRPIAVELYFLPIKSRVPLKFGPEITTEVTCARVKLTV
                     ENPQGKRGCGWGETPLSVQWVWPSSLSYIDRHNALVSLCLKIAEAWLSAPIEPGHPME
                     VGYHFLENVLPDLTSQFNRSQRHAPEPVPWLAALVCASAFDLALHDAYGMVHRLPTYS
                     TYNDQYMNFDLAHYLEPAPDAALSFANRFPEEFLSVDPQKTVPAWHLVGGKDPIEPDE
                     LTGSEPDDGYPILLRDWVRRDGLKCLKVKLRGDDFAWDYDRLSKVGQIAIEQRVDWLT
                     ADFNCTVQQPEYVTAMLDRLLQEQPRVYGMLLYVEQPFPYDLEAHRIDVHAVSARKPL
                     FMDESAHDWRFVRLGRALGWSGVALKTCKTQTGAILSLCWAKAHGMTLMVQDLSNPML
                     AQIPHVLLAAHAGTIMGVESNGMQFYPAASIPEAAIHAGLYRRRNGCLDLSTVSGPGF
                     GYRVDQIKRELPAPAFP"
     gene            complement(3837..>5899)
                     /locus_tag="VHS97_03055"
     CDS             complement(3837..>5899)
                     /locus_tag="VHS97_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX3447201.1"
                     /translation="PPRRHEVPRVDKVSWCKNPIDAFVIAQLEKNGLRPADESDRWAL
                     LRRITFDLTGLPPTPGELAEFLADSAPLAYERLIDRLLASPRYGERWGQHWLDLARFA
                     ETDGFEFDQARPDAWRYRDWVVQALNHDMPYETFIRLQLAGDEVEPGDPAAFIATGFN
                     RCYPDMVDLNDQGLRRQNALNDITETTGLVFLGLTIGCARCHDHKFDPIRQTDFYGLE
                     AFFAPARFRDDYPIASGRDREENQKRAHDWENALAGVRTALIRLEGPWRDLLAPGTPA
                     GLDDDTATAFSKPDADRSPRDVELIYETLARDKRVKAGIWPIVLDPVTWNARRRLVAR
                     IEWIKRSTPPTLPKARGVDEAGPLAPPTYLLRRGEYGARGPEIKPSFPAVLCSLDEKS
                     DANPIPSSRSTGRRKALADWLVRPDHPLTARVMVNRLWRHHFGKGLVGTPSDFGTMGD
                     EPSHPELLDWLATELVARGWSLKSIHRLILTSATYRQSSRVQGEGTVVDPDNALLWRQ
                     NRWRLDGEAVRDSLLAVSGRLNQAMGGPSVFPELPPELKRLSSKGAVWPVSANVEDRE
                     RRSLYVFVRRNLRFPFFEVFDRPDTNASCPQRPVTTIAPQALSLLNSPLAHDAARRLE
                     TRAIREAGPDRAKEVEWVSLVVFGRSPDAAERRMMDEFLGGGGSLEHLCLALFNTNAF
                     IYID"
BASE COUNT         1114 a         1886 c         1804 g         1095 t
ORIGIN      
        1 cgtgaacggc ttcgagctgg gtttggacgg ctgggtctat ggcgccaacg gcgacagcgg
       61 cggcacagtc cgttctgtca aaacgcgcaa gactatcgag atcagcggcc gtgatttccg
      121 atttcgaccc gacaccggag atttcgaagc cgagagcggg cggacgcagt acggtcgcca
      181 ccgcgacgac tggggccatt ggttcggcaa caacaaccct aattggggct ggcactatgt
      241 cctcgccaat cgcgacctca gccgcaaccc ggatttcgcc ccacccgata cgcgagtgac
      301 gcttgagccc gacacccggc tgtttcccat cagtcgtacg ctggcccggt ttaacgaccc
      361 cggcgccgcc aaccatgtca cctcggccaa tagcccgacg ccctatcgcg acgagctctt
      421 cggtgccgaa ttcgccacca gcctgtttgt cagtgagccg gtacacaacc tggtgcatcg
      481 aatggtgctc gagccccagg gcatgagctt ccgcggccat cgcgggccga acgatgcaaa
      541 tcgcgagttt ctcgcgtcca gcgacaactg gttccggccc gccatgctca aaacaggccc
      601 cgacggtgcg ctatggattg ccgatatgta tcgcgctgtt atcgagcacc ccgaatggat
      661 tcctgacgat tgggaacggc ggctcgacct tcgcgccggg agcgaacagg ggaggatcta
      721 tcgcgtctac ccgctcgaca aaaagctccg gcccatccca cagttcgaca agctcgatac
      781 gacccagctc gtggccgcga tggaaagtcc gagcggctgg cagcgtgaca cggtacagcg
      841 cctgctcttg caccgccgtg atcccgcggc gatcgcccct ctacgagctt tggcgatcgc
      901 cacgaaacag cccaagacgc gtgtccaggc gatctggacc ctggccgatc ttaacggcct
      961 cgatgaacca tgtgcgctgg ccggcctcga cgacccagat ccccgtgtgc gcgagagcgt
     1021 gatcgcggcg gtgtcatcac gcgcggggca gtctcggatt atcatggatg ccttgattaa
     1081 gacggctgag gacgccaatg ctcacgtccg attacaggca gcgctggcgc tgggcgactg
     1141 cgcggaccgc cgcgccggcg aggcgttggc ggcactggcg cggcgcgatg gcaacgacca
     1201 atggttgcga gccgcgatct tgtgctcggc ggttccacac gtggacacac tgttagtcgc
     1261 gctgctccgt gacacagcac ccggcgttca aaagccagag tcgttcgaag caatggtcgg
     1321 tccgctgttg agcgtggcag tttctcacag cgatcgagca ccgatcgagc tgctcacgcg
     1381 ctcgatcgcc gtgccttcgg ggcaaggggg gcggtacgct ccgtggcagt tttccacact
     1441 cgcgggattg ctcgaagcgc gcgatcaccc caacaggcca ttcaagcttg atctcgacaa
     1501 gccatttgcg tcggtctggg cggctgccca ccgcgtcgta tccgacgaat cggcccccga
     1561 agccgagcgg gttggggctg tccggcttat cggctaccgg gcgcggcgag atctcaagaa
     1621 tcgcgacttg ctcatcggcc tgttgcggcc ccaggtctcg gctggcctcc agcaagcagc
     1681 cgtggccgcc ctggggcgaa ctgatgaccc caagctcgct gacctcttgc tggtcgattg
     1741 gaagagatat tctccctcga ttcgagactc gattcttgac gtcgttctca gccgaacggc
     1801 gtggacttcg tcgttgctct cttgtctcga agacggctgc gtcccgcccc ccgagatcga
     1861 ccctgcgcgg cggcagcagc ttctcacgcg caaaagcgat cggctcaggt cccgcgccga
     1921 ggctgtcttc gcgcatcaaa gccaaccgcg gcaagccgtc gtcgacgcct accgggcgtc
     1981 acttgcgatg cgcggcgaca aatcggcggg tgcaaccgtt ttcaagaagg tgtgtgcgtc
     2041 ctgcaatcgt ctcgggaacg aaggggtcga ggtcgggccg gacctcgccg ccttgaatga
     2101 caagtcgcct gaggcactct tgatcgccat actcgatccc agccgagcgt tcgaagcaaa
     2161 gttcgccagc ttttcgatcg caactgtcga cggccgggtc atgaacggac tgatcgccgc
     2221 cgagtcggcc acggccgtca ccctccgccg acaggatggc aaggaagacg tgctgctccg
     2281 gtctcagatc gaggagatga ctggctcaag ccagtcgctt atgcctgaag gactcgagaa
     2341 agacctgaag ccgcaagacc tggccgacct gatcaccttc gtggtctcgg ctcgcccctc
     2401 gcttaagcca gagtgaccca tcatgcccct gcccaccgat atccggccca tcgccgtcga
     2461 gctctacttc ctgccgatca agtcccgcgt gccgctcaag ttcgggcctg agatcacgac
     2521 ggaggtgacg tgcgcacgtg tgaaattgac tgtggaaaat cctcagggca agcgcggttg
     2581 cggctggggc gagacgccac tgtcggtcca gtgggtctgg cccagtagtc tcagctatat
     2641 agatcgccac aacgcactgg tctcgctttg cctcaagatc gcggaggcct ggctgtcggc
     2701 acccatcgaa cccggccacc cgatggaggt cggctatcac ttcctcgaaa acgtgttgcc
     2761 tgatctgacg agccaattca accgatccca acggcacgca cccgagcccg ttccgtggct
     2821 cgcggccttg gtttgtgcat cggccttcga tctggcgctg cacgacgctt acgggatggt
     2881 ccaccgcctg ccaacatact cgacgtataa cgatcaatat atgaactttg acctggctca
     2941 ttacctcgag ccggcgccgg acgctgcgtt gtcgttcgcg aaccggttcc cggaggaatt
     3001 cctgagcgtt gacccacaaa agaccgtccc cgcctggcac ttggtcggtg gtaaggaccc
     3061 tatcgaaccg gacgagctga caggcagcga acctgacgac ggctatccta ttcttttgcg
     3121 cgactgggtt cgccgcgacg gcctcaagtg cttgaaggtc aagctccgtg gtgatgactt
     3181 cgcgtgggac tacgaccggc tgtcgaaggt cggccagatt gcgatcgaac aaagggtcga
     3241 ttggctgacg gccgatttca attgcacggt ccagcagccc gaatacgtca cggcaatgct
     3301 cgatcgcctg ttgcaggagc agccgcgcgt ctacgggatg ctgctctacg tagagcagcc
     3361 tttcccctac gatctggagg cccatcgcat cgacgttcat gcggtatcgg cgcggaagcc
     3421 attgtttatg gacgagagcg cacacgactg gcgcttcgtc cggctggggc gcgcgctcgg
     3481 ttggtcggga gttgcgctca aaacctgcaa gacgcagacc ggtgcaatcc tctctctctg
     3541 ctgggcaaag gctcatggca tgacgctcat ggtccaggac ctctcgaatc ccatgctcgc
     3601 gcaaatcccc cacgtgttgc tggccgctca cgcggggacg atcatgggtg tcgagtccaa
     3661 cggcatgcaa ttctacccgg cggcctcgat cccggaggcg gccatccacg ctggtcttta
     3721 tcgccgccgt aacggatgtc ttgacctgtc aaccgtcagc gggccgggct ttggatatcg
     3781 cgtcgatcag atcaaacgcg agctgccggc gccggccttt ccttgacctg tcgagatcaa
     3841 tcaatataga tgaacgcgtt tgtattgaaa agtgccagac aaagatgttc gagcgagccg
     3901 ccgccaccca ggaattcgtc catcatccgc cgctcggcgg cgtcgggcga gcggccaaaa
     3961 acgaccaatg aaacccactc cacttccttg gcccggtcgg ggcccgcttc ccggatagcg
     4021 cgagtctcga gtcgtcgtgc ggcgtcgtga gccaacgggc tgttcaacaa gctcagggcc
     4081 tgcggagcga tcgtcgtgac cggccgttgc ggacagctcg cattggtgtc tggccgatcg
     4141 aagacctcga aaaatggaaa tcgcaagttg cgcctgacga agacgtataa actgcgacgc
     4201 tcgcggtcct cgacattcgc cgagaccggc cagactgccc ccttgctgct caggcgtttc
     4261 agttcggggg gaagttcggg aaaaacggac ggccccccca tggcttggtt gagtcgcccc
     4321 gacacggcca gcaaactgtc gcggaccgcc tcgccgtcca gccgccaccg attttgccgc
     4381 cacaagagtg cgttatcggg gtcgacgact gtcccctcac cttggactcg cgacgactgg
     4441 cgataagtcg cgcttgtcag aatcaggcgg tggatcgact tcagactcca gccgcgcgcg
     4501 accaactcgg tcgccaacca gtcgagaagc tcagggtgcg acggttcgtc gcccatcgtg
     4561 ccaaagtcac tgggtgtgcc gaccagaccc ttgccgaaat gatgccgcca gagccggttc
     4621 accatcaccc gagccgtgag cggatgatcg ggccgaacca gccagtcggc cagggccttg
     4681 cgccggccgg ttgaccgaga tgatgggatc ggattcgcgt cggatttctc atcgagagaa
     4741 cagagtaccg ccgggaaact cggcttgatc tcaggccctc gagcaccata ctcaccgcgg
     4801 cggagcaagt aagtcggggg tgccaaggga ccggcctcgt cgacaccccg ggctttcggc
     4861 aaggtggggg gtgtcgaccg cttgatccac tcgattcgcg ccaccaggcg acgacgcgcg
     4921 ttccaggtga ctggatcgag tacaatgggc cagataccgg ccttgactcg cttgtctcga
     4981 gccagcgttt cgtaaatcaa ctcaacatcg cgtggagaac gatcggcatc gggcttgctg
     5041 aacgcggtcg ccgtgtcgtc atcgaggccg gccggtgttc ctggtgcaag cagatcgcgc
     5101 cacggcccct ccagccggat caaagcggtc ctgacgcccg cgagggcgtt ttcccagtcg
     5161 tgggcgcgct tttgattttc ttcccggtcg cggcccgagg cgatcgggta atcatcgcga
     5221 aatcgcgctg gcgcaaaaaa ggcctccagc ccgtagaagt cggtttgccg gatcgggtcg
     5281 aacttgtggt cgtggcaccg cgcacaaccg atcgtcaagc ccaggaagac cagtccagtg
     5341 gtctcggtga tgtcgttgag cgcattctga cgcctgaggc cctggtcatt cagatcgacc
     5401 atatcgggat aacagcgatt gaagccggtg gcgataaaag ccgccggatc acccggctcg
     5461 acctcgtcgc ccgcgagttg caaacggatg aaagtttcat aaggcatgtc atgattgagc
     5521 gcctggacca cccagtcgcg gtagcgccag gcgtcgggac gagcctggtc aaactcgaag
     5581 ccgtcggtct cggcgaatcg tgccaggtca agccagtgct gcccccaacg ctcgccgtag
     5641 cgcgggctcg ccagcaggcg gtcgattaac cgctcatagg ctaagggcgc cgagtcggca
     5701 aggaattcgg caagctcgcc gggcgtagga ggcagcccgg taagatcgaa ggtgatccgt
     5761 cgcagaaggg cccaacggtc ggattcgtct gccggacgca ggccattttt ctcaagctga
     5821 gcgataacga acgcgtcgat cgggttcttg caccacgata ccttgtcgac acgcggcacc
     5881 tcgtggcgcc gcggcggca
//
LOCUS       DATAQL010000186         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_479141, whole genome shotgun sequence.
ACCESSION   DATAQL010000186 DATAQL010000000
VERSION     DATAQL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479141"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(728..1732)
                     /locus_tag="VHS97_03060"
     CDS             complement(728..1732)
                     /locus_tag="VHS97_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3447202.1"
                     /translation="MKAAVFESFGEPSQMLNVREVSDPTPGPGEVRVRMILSPINPSD
                     MLVVQGLYGVLPKLPATPGFEGVGIVDDVGPGVLARVLNLKGKRVAAINSAGGNWAEM
                     AIIPARQARPVPDDIPDEQAACYFVNPATVLAMARHVLAVPKGDWLLQSAAGSALGRM
                     LIKLGRHDGFKTLNVVRRHEAIAELKDLGGDAVISSSDGPIDEQVRRLTGGAGARYAV
                     DPVGGETGTGVFRSLAEGGRVLLYGTLSGEPIQVDPRLVISGPRSVEGFWLGHWMRER
                     SIPSALLLFREIAALMREGVLRSEIGRVYPLSQIKEAAREAGIVGRQGKVLLRLGQVG
                     "
     gene            complement(2109..>3146)
                     /locus_tag="VHS97_03065"
     CDS             complement(2109..>3146)
                     /locus_tag="VHS97_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3447203.1"
                     /translation="AIMETYSPQQLTMKSGLAANFAVSDAWYASAPCQTWPNRAFVHL
                     GTSCGRVNNWPDDPFDFNVPTIFNVLEANKNTWSVFNPGPIISLTRLQFPKLWDSGLE
                     SHFLSIKEFLKQAQEGTLPSYSFVEPSFLGIEGTPNDEHPPHDVCLGEQFLQSIYSAV
                     VNGKNWDKTLLVVTYDEHGGCPDHMPPPWGAITPDKQSDPGEEGFRFNRFGVRVPTIL
                     VSPWIQAGTVFRSTTNVPFDHTSILATLRDWLAIPDQLMLPSKRVQAAPTFGDVLNLS
                     QVRPDKPVITASCGPSPRLRDAMEPPNDLQKSMVAAMIRKIVAHPVDLSLVRKVLATI
                     RTREDAAQFLA"
BASE COUNT          626 a          975 c          957 g          588 t
ORIGIN      
        1 gaaatcgcgc gtaccgtcgg acgatcttca tcccggatga cttcgcccca agctccggtg
       61 taggcggctc gcacttgcga ggaactctct cccacgcaga gcagccgctc gcccgtctgc
      121 gacaggcagg ctaccgcccg ccaatcctgg gcaccagtcg taccactact catcttccat
      181 cactcccacc cggcactttc ccctcaacag ccgaatcatg gttttcggcc tcattctgaa
      241 atcgtcgcgc cgggacttgg tctttgattc atcgtctaga aaaagtgtcg tgagaccgta
      301 ttgccttcca gcaatcagta cggattaaat taggcagacg ttcaacaaag agtgaaacag
      361 ccgtaaaacg gcgcggcgaa cgctgcaaga tagcgtgatt gtggtggaat ccggcgtcca
      421 aagtattccc gtgcacgatg actcacgcac gcatccgtcg taaccgcccg atcgaaaact
      481 gcgagagcct gggaaacgaa aagaccagat cgacgccagg ttgattgatc cgggtcgtga
      541 cgtacgtgtt acggcatcag ccggagtctc tacagaacat aattgtagct aacgcacgcc
      601 gtttcggcga atgaaattta tgaaattcga gtggaaacgg tggaggtcgg aaccaccgcg
      661 cggcgggcag tggctcgaga acgtcgggat cgcgtcgcaa tcgatcaggg tgtgtccgta
      721 gcgacaatca tccgacttgg ccgagcctga gaagtacctt tccctgccgg ccgacgatgc
      781 cggcctcgcg ggcggcttcc ttgatttggc tgagtggata aacccgaccg atctcgctcc
      841 ggaggactcc ctcgcgcatg agcgccgcga tttcgcggaa caagagaagg gctgagggaa
      901 ttgaacgctc tcgcatccaa tggcccagcc aaaacccttc gacggatcgc gggcccgaaa
      961 tgacgaggcg agggtcgact tgaatcggct cgcccgaaag cgtcccgtac aggagtaccc
     1021 ggcccccctc ggccagggac cggaaaactc cggttcccgt ttcgcccccg accgggtcca
     1081 ccgcgtaccg cgctcccgcg ccgccggtga gcctgcgcac ttgctcatcg atcggcccat
     1141 ccgagctcga gatgaccgcg tcgccgccca gatccttgag ctcggcgatt gcctcatgtc
     1201 ggcggaccac attcaaggtc ttgaatccat cgtgacggcc cagcttgatc agcatccgac
     1261 ccagggcgga accagccgcc gattggagca gccaatcgcc tttaggcacg gccagaacat
     1321 gccgtgccat cgccagcacc gtggccggat tgacaaaata gcacgcggcc tgttcgtcgg
     1381 ggatgtcgtc ggggacaggt cgcgcctgcc gggcgggaat aatggccatc tcggcccaat
     1441 tgcccccggc ggagttgatt gcggcgacgc gcttgccctt gagattgaga acccgtgcca
     1501 gcacgccagg acccacgtcg tcaacgatgc cgactccctc aaagcctggt gtggccggca
     1561 atttcggcaa tactccgtag agaccctgca caaccagcat gtccgacgga ttgattggac
     1621 tcaaaatcat ccgtacgcga acctcgccgg gccccggcgt ggggtcggag acctcgcgca
     1681 cattgagcat ctgggatggt tcgccaaatg attcaaaaac cgcggctttc atgcgggcgt
     1741 cccgatcagg tcgaatggct ccgaggtcga caccgaggcg gctatcttac gaactgagac
     1801 cacgtccgtc acgtagggtg gtgccgaacg atccaaagga tcgtgaggcc caccgacgaa
     1861 aaccacggcc caccgtgaaa accacggccc gtccggtggg cctcgccgcg atttctcgcg
     1921 gctccgcggt cctgcacggt gggcctcgcc gcgatttctc gcggctcggc gatcctgcac
     1981 ggtgggcctc gccgcgattt ctcgcggctc cgcggtcctg cacggtgggc ctcaccgcga
     2041 tttctcgcgg ctccgcggtc ctgcacggtg ggcctcgccg cgatttctcg cggctcggcg
     2101 ccaccctact acgcgagaaa ttgagccgcg tcctcgcgag ttcggatcgt tgccagcact
     2161 ttccgtacga gcgaaaggtc gacaggatgg gcgacaatct tacgtatcat cgccgcgacc
     2221 atgctcttct gaaggtcgtt cggcggctcc atggcatccc gcagacgagg cgacgggccg
     2281 cagctcgcgg ttatcacggg tttgtccggg cggacctgcg acaggttcaa cacgtcaccg
     2341 aacgtcggcg cggcctgaac gcgtttgctg gggagcatga gctgatcagg gatcgccagc
     2401 cagtcacgca gcgtcgccag gatggaggta tggtcaaacg gcacattcgt cgtggaccgg
     2461 aagacggtgc ccgcctggat ccagggcgac accaggatgg tcgggacgcg gacgccgaag
     2521 cggttgaagc ggaagccttc ctcgcccgga tcactttgct tatcgggcgt gatggctccc
     2581 caagggggcg gcatatggtc ggggcagccg ccgtgctcgt cgtaggtaac gacgagaaga
     2641 gtcttgtccc agttcttgcc gttgacgacg gccgaataga tcgactgcaa gaattgctcg
     2701 cccaggcaga catcgtgggg tggatgctcg tcgttcggcg ttccttcgat acccaggaac
     2761 gacggctcga caaacgagta cgacggcagc gtgccctcct gggcttgttt cagaaactct
     2821 ttgatgctga gaaaatggct ctcaagcccg gaatcccaca atttggggaa ctgcagacga
     2881 gtcagcgaga tgatcgggcc agggttgaac acggaccagg tgtttttgtt ggcttcgaga
     2941 acgttgaaga tcgtcgggac attaaaatca aaaggatcgt cgggccagtt attgacccgg
     3001 ccgcacgagg tccccagatg gacgaacgcg cggttcggcc aggtctggca cggtgccgag
     3061 gcgtaccagg cgtcgctgac ggcaaagttg gctgccagcc cgctcttcat tgtgagttgt
     3121 tgcgggctgt acgtctccat gattgc
//
LOCUS       DATAQL010000187         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_936698, whole genome shotgun sequence.
ACCESSION   DATAQL010000187 DATAQL010000000
VERSION     DATAQL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936698"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHS97_03070"
     CDS             <1..691
                     /locus_tag="VHS97_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX3447204.1"
                     /translation="AAGWDFLSATITITDPPRDLVAGMIAVVRIKVPMAGIEPFCSAP
                     IDPPRLVAGESRQAYVCPDHPANLTAEPGRCPVDHKDREPRPLYDYQRLRWWCPMHPS
                     VTADLAGASCKACGGMILVPRVISYSPAGQVLSVPQSAVVDAGACKVVFVESMPGMFD
                     GVEVVLGPRCGDSYPVVRGLEPGQRVAIAGAFLLDAETRLNPSLAAGYFGAGRGERVV
                     VPGPIAGANKR"
     gene            735..1700
                     /locus_tag="VHS97_03075"
     CDS             735..1700
                     /locus_tag="VHS97_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3447205.1"
                     /translation="MIDAIITFSIRNRALVIGASLVLAALGAWAAWDTPVDAIPDLSE
                     NQVVVFTEWKGHGPREIEDQVTFPLTLGLRGLRGVRVVRSSSDVGFSMISVILGDQVK
                     LSEGRRRVAERLARVQAQLPREASPELTPDAAATGQIFWYTVEGAGFDLGRLRAIQDW
                     YVRPQLGSVAGVADVSSVGGFPIEYQVIHDPNRLRIFGVSLSDLVEAVSTSNASSGGH
                     VIHKGNAEYVVRGVGRLGASLTPGDESFDPDRAIRDLENVVIPLAKGGTIRLAAVADV
                     ALGPGFRRGVLEKDGNEVTGGVVLMAHGENPLEVTRRIKVKIREL"
BASE COUNT          293 a          590 c          590 g          351 t
ORIGIN      
        1 cgccgcgggc tgggacttcc tgagcgccac aatcacaatc accgatcccc cgcgcgacct
       61 tgtcgccggg atgatcgcgg tcgttcgcat caaggttccg atggccggga ttgagccttt
      121 ctgctctgcg cctatcgatc cgccccggtt ggtcgcgggc gagtccagac aagcctatgt
      181 ctgtcctgac catcccgcca acctgaccgc cgaaccgggc cgctgtccgg tcgaccacaa
      241 agatcgcgag cctcgaccgc tctacgacta ccagcggctg cggtggtggt gcccgatgca
      301 tccgagtgtg acggccgacc tggctggggc aagctgcaaa gcctgcggcg gcatgattct
      361 cgtgcccaga gtgatctcat acagtccagc gggccaggtg ctgagcgtgc cgcaatcggc
      421 ggtcgtcgac gccggtgcgt gcaaggttgt cttcgtcgag agcatgcctg gcatgttcga
      481 cggcgtcgag gtcgtcctgg gtccgcgttg cggcgactct tatccagtcg ttcggggcct
      541 tgaacctggc cagcgcgtcg cgatcgccgg cgcgtttctg ctcgatgccg agacacgcct
      601 gaaccccagt ctcgcggccg gctacttcgg agcgggccga ggcgaacgcg tcgttgttcc
      661 tgggccgatc gccggagcca acaaacgttg agccttgagg cacgggcatg ttgctcgcgc
      721 cacaatcaaa tcccatgatc gatgcgatca tcaccttctc gattcggaat cgtgccctcg
      781 tgattggcgc cagcttggtg ctggccgccc tgggcgcatg ggccgcctgg gatacgccag
      841 tcgacgcgat tcccgacctc tcggaaaacc aggtcgtcgt gttcaccgag tggaagggac
      901 acggcccgcg cgagatcgag gaccaggtga cctttccgct cactctcggc ctccgcgggc
      961 tgcgcggtgt gcgcgtggtg cggtcgtcca gtgatgttgg tttctcgatg atcagcgtga
     1021 tcttgggcga tcaagtaaaa ctctccgaag ggcgccggcg cgtggccgag cggctggctc
     1081 gagttcaagc ccagctgccg cgtgaagcta gccccgagct tacgcccgac gcggcggcca
     1141 ctggccagat cttctggtac accgtcgagg gcgccggctt tgatctgggc cggctccggg
     1201 cgatccagga ctggtacgtt cgcccccagc ttggctcggt cgccggagtg gccgatgtct
     1261 ccagcgtcgg tggtttccca atcgaatacc aggtgatcca cgaccccaac cggctccgaa
     1321 ttttcggcgt atcgctcagc gacctggtcg aggcagtctc aacatcgaac gcgtcgtccg
     1381 gcggacacgt catccacaag ggaaatgccg agtacgtggt gcgtggtgtt ggccgtctgg
     1441 gcgcgtcgct tacgccgggt gatgagagct ttgaccccga tcgcgccatc cgcgacctgg
     1501 aaaacgtggt tattccgctt gccaaaggag gcacgatccg gcttgccgcg gtcgcggatg
     1561 tcgcacttgg cccgggcttt cgccgaggcg tcctggagaa agacggcaac gaggtcactg
     1621 gcggcgtggt cttgatggcg cacggtgaaa acccgctcga ggtcacgcgc cgcatcaaag
     1681 tcaagatccg cgagctttag cccggtctgc cgcgcggcgc gcgcatcgtc ccattttacg
     1741 accgcacccc gctgatccaa ggggcgatca gtacggtcac acataccgtt gtcgaggcca
     1801 tgatcgcggc atcattgtgc gtgc
//
LOCUS       DATAQL010000188         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1089190, whole genome shotgun sequence.
ACCESSION   DATAQL010000188 DATAQL010000000
VERSION     DATAQL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089190"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..513)
                     /locus_tag="VHS97_03080"
     CDS             complement(163..513)
                     /locus_tag="VHS97_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3447206.1"
                     /translation="MRPIYHPHPDEITVQGILYALSDPVRVRILNGLRDADCSQNCTT
                     FTNIGETPLPKSTLSQHFRILREAGLVRSERKGVELQNRMRCEELEPKYGPMIRAILS
                     AYQADCAAKSEAAD"
     gene            795..1523
                     /locus_tag="VHS97_03085"
     CDS             795..1523
                     /locus_tag="VHS97_03085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3447207.1"
                     /translation="MAVITGGSSGIGLATAQRFVAEGAYVFITGRRQTELDKAKALIG
                     KNVTAVQGDIASLEDLDRLYATIKKEKGILHIVVASAAMVELVSPSTATPEHYDKTYN
                     TNARGTFFTIQKAVPLMTAGGAIVTVGSVAYKKGLPPMTVYSSTKAAIRYSVKGWAAD
                     LIGKGIRVNNLSPGVIDTPIIDSQHETKEGADAMRDMYVKMTPLGRLGRPEEMASAIF
                     YLASDESSFTTGIDLVADGGLSEF"
     gene            complement(1905..2612)
                     /locus_tag="VHS97_03090"
     CDS             complement(1905..2612)
                     /locus_tag="VHS97_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447208.1"
                     /translation="MSTLASKRIWLREAMAWVGCAAVLFAALRADTFVTVLILAFPLR
                     LALIARYAGLGVARGECRAAILPSWVAFLGDETDERRFVRGLGLSLFVDALVVGLWPV
                     WREAALEVGSLAPDPWARVNSSVFLQDWRMIARDLDYWKRLWTWEAWSLARWWLLFGL
                     LAIATTPLSRRVRPRVEAAIRLLRFSPWLIVLEFGYLVGVWIARGKVGVVPEPSTEFA
                     SEWGFGSMAFSFMSPSG"
     gene            complement(2718..2873)
                     /locus_tag="VHS97_03095"
     CDS             complement(2718..2873)
                     /locus_tag="VHS97_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447209.1"
                     /translation="MLTYLTAPGLSDAQIQLISGHDSKKSLEVYQHLSLDAVNEAYQE
                     AILALGL"
     gene            complement(2867..3121)
                     /locus_tag="VHS97_03100"
     CDS             complement(2867..3121)
                     /locus_tag="VHS97_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3447210.1"
                     /translation="MDTKQCKIFINQGKGSKDRYIFLPKSFRLVLRSRLQANPKNRYR
                     FESQRCTPFAPRRMQQIVQAYRDRAGIAHHVHPICSVTRC"
     gene            complement(3212..3370)
                     /locus_tag="VHS97_03105"
     CDS             complement(3212..3370)
                     /locus_tag="VHS97_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447211.1"
                     /translation="MTVQQGDRPFARHWNGTVLPGAVVVVVVGSLGIFLYHAVENARN
                     AARSAATT"
     gene            complement(3429..4250)
                     /locus_tag="VHS97_03110"
     CDS             complement(3429..4250)
                     /locus_tag="VHS97_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447212.1"
                     /translation="MPTIQLGPHRVTRLIIGGNPVYGYSHFNKNFDRHMTAWHTPERV
                     VELLKRCEDCGLNTFQNSYSERTLADVERYRSAGGTMNWLCLGKPDWDQHPEFIDDAA
                     KHKPIGISPHGSLNDRLYRQKKYNILTDLLKRIRDRGVLVGLSAHDPTLIETAEDKGW
                     DVDYYMTSLYFRTRTKDELRAIVGNDLPLGEIYLPSDPPKMFGAIKATSKPCLAYKLL
                     AAGRRIGSAAEVRRCFEEAFANIKGNDAAIVGMYQQFGDQVAENAAIVREIGSRA"
BASE COUNT          983 a         1341 c         1347 g          910 t
ORIGIN      
        1 gtcgaaagaa acaccatcgc gccggattgg cgatcacgtc ggaggtgcgg gctggtgttg
       61 caatggttgc aatgcgtgtc cattcttccc ccgtgtccat ttttccccac cacaggacac
      121 aatttgcaat acatcgggtt gttctggact gatgtgaagg cgttagtcgg ccgcttccga
      181 ctttgccgcg caatcggctt ggtaggcgct caaaattgcg cgaatcattg gcccgtactt
      241 cggttcgagt tcctcacacc gcatgcggtt ctgtaactcg acccccttgc gttcgctgcg
      301 gaccaagccc gcctcgcgga gtattctaaa gtgctgtgac agcgtggatt tgggcagagg
      361 cgtctcgccg atgtttgtga acgtcgtgca attctgcgag cagtcggcat ccctcagtcc
      421 gttgaggatt cggacccgca ccgggtcgga cagggcgtaa agaatgcctt ggacagtgat
      481 ctcgtcaggg tgggggtggt agatcggtct catatggcaa ttttaacgga ggttcggcct
      541 tcgtccaata gttctattca accgaacaat cggaaatgac gtgtggtcgc tttttcgggc
      601 gttgtcaatt gactcttgac tccttcgtgc actagttcta aagtattgaa ctacagcaca
      661 aggagaattt tatgaggatc tgggcgggct gccttcgacc gcggccaagg cgctactggg
      721 agcaatgctg gtgaaggctt tcaaccactt gattgaactt aaaggagtta cgtatgagca
      781 agttgagtgg caaagtggca gtgatcaccg gtggcagcag cggcatcggt ttggcgaccg
      841 cacagcggtt cgtcgccgaa ggcgcgtatg tctttatcac cggtcggcga cagaccgagc
      901 tggacaaggc gaaggcgctg atcgggaaaa acgtgaccgc ggtccagggc gatatcgcca
      961 gcctggaaga cctcgaccgc ttgtatgcga cgattaagaa ggagaagggg atactgcaca
     1021 tcgtcgtggc ctccgcggcg atggtcgagc tggtttcgcc ctcgacggcc acgccggagc
     1081 actacgacaa gacgtacaac accaacgccc gcggcacgtt cttcacgatt cagaaggccg
     1141 tgccgctgat gacggccggc ggggcaatcg tcacggtcgg ttcggtcgcc tacaagaagg
     1201 gccttccccc catgacagtt tattcttcga ccaaggccgc gattcgctac tcggttaagg
     1261 ggtgggcggc ggacttgatc ggcaagggca tccgcgtcaa caacctcagc cccggcgtga
     1321 tcgacacgcc gatcatcgac agccagcacg agaccaagga aggggccgac gccatgcggg
     1381 acatgtacgt caagatgacg ccgttgggcc ggctcggccg gccggaggag atggcctcgg
     1441 cgatcttcta cctcgcctcg gacgagagca gcttcaccac gggcatcgac ctggtcgccg
     1501 acggcggcct gtcggagttt tgatgaccgc tgctgcctac ccgccggttt ttgaccggcg
     1561 ggctccgaac acgctgacca gaaactgacc ttttgcggtt ttcaggctct tctccgagtg
     1621 attcgagacg ccacgactcg gcctaaaccc aacattcgtg tcaccagccg caaagtaggg
     1681 ggcccggggc cgtgaaactg gggacatcga gtccaagttg agttatcgag gccacccgtt
     1741 gtgggccctc cagagcggaa agcccaacgc agaccaaaac attgatagtc caaccgcgac
     1801 aggtacgagc gcaagcgacc cgaggattgc ctcgacccat cgccatcccg gcactatgcg
     1861 gaaaaacgcc agtccgacga cgaagctcga caggccgcca cgtgttagcc agaaggggac
     1921 ataaaagaaa atgccatact tccgaaaccc cattcgctgg cgaactcggt agatggctca
     1981 ggtacgacgc ccaccttccc ccgcgcaatc cacacgccaa ccaggtatcc gaattccaga
     2041 acgatcaacc agggggaaaa ccgcaggagc cgtatggccg cctcgaccct cggcctcacg
     2101 cggcgtgaga gtggcgtggt cgcgatcgcc aggagtccaa acaggagcca ccatcgcgcc
     2161 agcgaccacg cttcccacgt ccagagccgc ttccagtagt cgaggtcgcg cgcaatcatc
     2221 ctccaatcct ggagaaacac cgaggagttc acccgcgccc acggatccgg tgccaggctc
     2281 ccgacttcca acgcggcttc gcgccagacc ggccacagac cgacgaccag ggcgtccacg
     2341 aatagggaga gacccagtcc acgaacgaac ctccgctcat cggtctcatc ccccaagaat
     2401 gcgacccatg acggaaggat cgcggctcgg cactcccctc gggcgacacc tagccccgcg
     2461 tatctggcta tcagagcgag gcggagcggg aacgccagga tcaagactgt cacaaatgtg
     2521 tcggccctga gagctgcaaa gaggacggcg gcgcagccga cccaagccat cgcctctcgc
     2581 agccagattc gcttggacgc aagagtggac atgggtatct cacaagagaa gatctcacga
     2641 tcatcgaccg ccgtaccctt caagctcaag gcccaaaccc accattcgta gcacctcacc
     2701 aggaacaaag tagcacgtca cagcccgagg gccaggatcg cttcctggta agcttcattg
     2761 acggcgtcca gggagaggtg ctggtagacc tccaggctct tcttgctgtc gtgtccagag
     2821 atcaactgga tctgcgcatc ggacaagccc ggcgcggtca ggtaggtcag catctggtga
     2881 cggaacagat ggggtggacg tgatgagcga tgcccgcccg gtcacggtac gcctgcacga
     2941 tctgttgcat gcggcgtgga gcgaacggcg tgcaacgttg agactcgaag cggtatcggt
     3001 tcttcgggtt ggcctgcaaa cggctgcgca acaccaagcg gaaactcttc ggtaagaaga
     3061 tgtagcggtc tttgctgccc ttgccctgat tgatgaagat cttgcactgc ttggtgtcca
     3121 tgtcttcaac ctttgtcgcc ccgttgtact tcccagcaat gcatgtcctt tgtcgccgtc
     3181 tttgtcgccc tttgttgccc cctttgaggt ttcacgtcgt ggctgccgaa cgggcggcat
     3241 ttcgtgcatt ctccaccgca tgatagagaa agatcccaag ggaaccaaca accacgacca
     3301 cgaccgcacc aggtagcacc gtaccattcc agtggcgagc gaagggtcgg tctccctgct
     3361 gaacagtcat ggtcggactc ctaattggaa ccagatgcgg cgatcaaagc caccggtcaa
     3421 tgatctcttc atgccctcga cccgatctcc cggacgatgg cggcattttc agcaacctga
     3481 tcgccgaact gctggtacat acccacgatt gccgcatcgt tgcccttgat gttggcaaac
     3541 gcctcctcga agcatcgccg aacttcggcg gcactcccga tccgacgccc agcagccaag
     3601 agtttgtaag ccaggcaagg cttcgaggtt gccttgatcg ctccgaacat cttgggaggg
     3661 tcggacggca gataaatctc gcccagaggc aagtcgttgc ctacgatcgc ccgcagctcg
     3721 tctttggttc gggttcggaa atagagagac gtcatatagt aatcgacatc ccagccttta
     3781 tcttcggcag tctcgatcag ggtcggatca tgggctgaga gtccgacaag gacaccgcgg
     3841 tcccggattc gtttcaacaa gtcggtcagg atgttgtatt tcttctgacg atagagccgg
     3901 tcgttcaagc tcccgtgggg ggagatgccg atgggtttgt gtttggcagc gtcgtcaatg
     3961 aactcgggat gctggtccca gtcgggtttc cccagacaga gccaattcat cgtgccgccc
     4021 gcagagcgat accgttccac atcggcaagc gttcgctccg agtagctgtt ctggaaggtg
     4081 ttgagcccgc aatcttcgca tcgcttgagc aattccacga ctcgttccgg cgtgtgccag
     4141 gcggtcatgt ggcggtcgaa gtttttgttg aagtgcgagt aaccgtagac ggggttgccc
     4201 ccgatgatta gccgagtgac tcgatgtgga cccagttgaa tggttggcaa tagcgacgac
     4261 gttcttgtgg gtccatcagc ggcttgaaca gagggggtca ttgcagcaac ggcggcagcg
     4321 atgcctgcag tttggtgcag gaaatcccgg cgactgggag ccgatcgaat gccatcagcg
     4381 agacgatccg atgaagaggt atccatgaga gaccctttcc tggctcggta gaagttgggg
     4441 atggcttcag catatcaggg tgcttccaga aagagccagc acacacctcg aagtcgggat
     4501 cgggggctaa acctgcggac atcgaatctt gattgaccta gaacgagagc cgctagatca
     4561 gatcaagccc gtaggtgatc c
//
LOCUS       DATAQL010000189        11709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2920711, whole genome shotgun sequence.
ACCESSION   DATAQL010000189 DATAQL010000000
VERSION     DATAQL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2920711"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VHS97_03115"
     CDS             complement(<1..214)
                     /locus_tag="VHS97_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447213.1"
                     /translation="MSTRVLPSVVHQVLDLPAASLASVIGAWPRSALLESGPGFSGAG
                     RWSILAAYPRLVWEATGSRWSLHADSG"
     gene            402..536
                     /locus_tag="VHS97_03120"
     CDS             402..536
                     /locus_tag="VHS97_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447214.1"
                     /translation="MAARSNRGRNRLCDVVPLALLTLSIVTPGAWGQVSLPDQGKVVT
                     "
     gene            600..1187
                     /locus_tag="VHS97_03125"
     CDS             600..1187
                     /locus_tag="VHS97_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447215.1"
                     /translation="MDLDEREFDDAGLSSLKGLTRLQSLSLDGTRITDAGLANLKGLT
                     SLIDLSLDNTEIGDAGLAQLEGLKNLQKLSIDRTRVSDAGLAHLKGMKRLESLSLVST
                     AVTDAGLAHLSGLTRLQWLLLHNTHIGDAGIKHLKGLTDLEFLFLHNTQVSDAGLEYL
                     KGLKRLQILNVENSRVTEAGVQQLQKLRPQLKISF"
     gene            1970..3256
                     /locus_tag="VHS97_03130"
     CDS             1970..3256
                     /locus_tag="VHS97_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX3447216.1"
                     /translation="MRTMKVRALDGRWRSWAPSWLASHRPSRLALPQRPTVSRSSGPA
                     ARTISREDLKKRIATAVLAFRGYDLTNLGRSPELLEHRVYGPVVRAALESASALCGDV
                     LGQKVDLAARVLAREPSTLETFVHDIGTIVSMELAQIRLLEEIFEIPVPHARLSFGHS
                     IGELSALVVGGMYTMEQLLPIPLALATDCAELTANTTMGILSTQGNTLQIEDVQHLCS
                     SISSRGQGLIGPSTYLSPFQVLLLGQGNTLDLLEQEMGEFLPTGVTLRRKPNRWPPLH
                     TPLVWERNIPNRTAMMMYHTAGGLQKPTPSVLSCTTGLANYDAWNSRAILADWTDHPQ
                     RLWDALEYSLASGAELVIHVGPDPKLLTTCFDRLSHRIMKQLKMRHLDRLGSSVIPSI
                     GRNGWLTRKLPANAVLLRAPFLDHLVLEDWLLAQDV"
     gene            3404..5317
                     /locus_tag="VHS97_03135"
     CDS             3404..5317
                     /locus_tag="VHS97_03135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3447217.1"
                     /translation="MSTQTREPETQTFVEAALKLGGKSEEEARTTGTVDRADDQVDAL
                     FAARYQTANSPAHRIVWDREATLDLFLPQPPATSPASEKIMQRSLEAVRKNREQGSLY
                     GPDGKVSEAVLNDLSQAGYWGLLIAPEYGGGGASFASFARFLTKMATIEPNLAGLASV
                     HGCIGAVDPLRTFGNSEQKERYLPRLASGERLSAFALTEPGAGSDLTALKTQAVLDGD
                     DYVVNGEKLFITNAVPGRTIGLVCLIDKRPAVLIVDLPDHEDEHFQLVTYGLLALRHL
                     NNHGLRFRDFRVPRENLLQPAQGDGLTIAYHGLNLGRVALCATAAGSMRVMLANMLPW
                     AKFRRTYGAAIVSRELVERRIGRMAALIVGCDAMVDWCSWLLDQGFRGELECIIAKIF
                     GSESQKEAAVELFMKTHGGRAFLKGHLFGDNIHEYLAPCIYEGEGEMLGMAFFKSLIK
                     EHGKEFFEPIGRAIQASGIKKPNPMNPAHAWALRSALVPYAKWRTAQMVTGKRRLNFD
                     SLSLPPALAAHAEYAAEALENGRLEISDVMTKHQLRLADRQCRMAELSQRLQDLVVIL
                     ATSLWASRQSNEVVQSAADILCQDLTRKITCKRPTDAYFRAVTKLGETVANGGFEAIA
                     GIEAGEILMPYNA"
     gene            5336..7489
                     /locus_tag="VHS97_03140"
     CDS             5336..7489
                     /locus_tag="VHS97_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX3447218.1"
                     /translation="MANAFRFDELDGKIGVVTFDLPDKKVNTLSQEVLKELAGLVAEL
                     EKRTDLVGLLFKSGKPGQFVAGADLNELAMMSYAKLEQVAGLTNMGHQLYNRISRLPF
                     PTVALIDGGCLGGGTELVLTMDERLASTAPHTQISLPEVSIGILPGWGGTQRLPRLIG
                     LNAAIEVICAGEKLSGQKAVTVGFAFDAVPAEKLVEEGRRLIEYLQQSGEWKSQRERL
                     QGPLGLNADELNFAFAVAEGAIKGKTKGHYPAPLVALKAIREGCNKTLEEGLKAEQNG
                     FGELAGSPIAANLIGIFFLKNRLARDPGVSDPSVKPRPVHSVGVLGAGFMGAGIAAAN
                     ARSAIRTSMVDVDDARIADGLKRASDVVMSRIKIGRATPQDMASMLALLNTSTSTKIF
                     ADSDIVIEAITENEAVKKEVYRGLAGVLKDDAILASNTSTISITRMAESAPHPERFIG
                     MHFFNPVDRMELVEVIRGEKTSDETVATVVALAKRIRKTPIVVKDCAGFLVNRVLFPY
                     MNESLILLQDGASVEAIDRAATKFGMPMGPMILTDLVGLDTAVYAGKVVSKAYPDRTV
                     TSPILEEMLKASPGDKKSLMKFWLSDKKAKTQPNPAALAIIAKYRTGDRAIESDEITD
                     RLFLPMLLEATRVLEEGIVREPGDVDMGLILGIGFPPFRGGILRWCDSLGAAAVVDKL
                     AKYAHLGKRFEPTESLLKLAKTGETFYPRPKLVKG"
     gene            7565..8722
                     /locus_tag="VHS97_03145"
     CDS             7565..8722
                     /locus_tag="VHS97_03145"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX3447219.1"
                     /translation="MKNAVVIDAVRTPVGRASSDRGYYRDVRSEDLSAHVIQALLKRT
                     GVAPKEIEDVRWGCVMQQGEQGFDVARIAAMVAGLPPETGGVTINRNCASSLQAINDS
                     AMSIAAGCEDVQIAGGVEHMDHVPMTKDYNPCPSLFRHHSEAIMNMGLTAEYLAAKYR
                     IPRGKQDEFALRSHQLATKATDAGEFKNEIIPTWGRDDAGRKVLLTTDQCIRRDSSAE
                     SLAALPPAFNPEGGSVTAGNSSPINVGAAALLVMSEEKARELGLKPIARIRAMAVAGV
                     NPEEMGIGPVPATQKALKRAGLTLDQIDCFELNEAFAVQVLSVLKLLGLGEEKVNKRG
                     GAIAIGHPLGASGARIATTLLHRMRDEGAKLGLASMCIGQGQGVTTIFEAC"
     gene            8723..9511
                     /locus_tag="VHS97_03150"
     CDS             8723..9511
                     /locus_tag="VHS97_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3447220.1"
                     /translation="MIRHPLGLRLDSDRSVRDQIYESARMGARGVVIDASDDVAPHRL
                     GATGRRELRQILRTVELSLVALSLPTRRPFDSADQLDDRMRRADAAFAMAYELGTTIV
                     LARAGAVPPPDDNARLEVFAGTLGELGRRAEHHGVRLALETGSEPGQKLKEFLDALGS
                     PGLAASIDPASILQAGIDPIVTVRELASWVVHAYAKDATGSSGVKSVNPRGFGYPPGA
                     LDWEEYLGSLEEIGYHGFLTVWPAPGLPAAAQFTAVSDRLKQVL"
     gene            complement(9529..>11709)
                     /locus_tag="VHS97_03155"
     CDS             complement(9529..>11709)
                     /locus_tag="VHS97_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447221.1"
                     /translation="EIVSLAHGPGEDAARIERFFDGEGPELAFSPDGKYVVVATRPPR
                     IWDASTGRPLHQLSGHAGSVPGVAFSPDGRQVATAGVDSTIRLWDVQTGTERAVLRGH
                     SALVGCVAFHPDGWCLLSGGRHIGQVKLWDLTHPQENLTLTDCHSPAFAFVDDGRKLS
                     LVTNLGGLQVREIASGSTRVGAQVDMTMQWLTPAAPAEFSLEGRRFAAVSSNRLFVKV
                     CDPQSGTELIALRGLSAPATFVSLSRDGSRAAASGLYAKNEVRSREVKVWDATSGQIL
                     ASFKPSNAPTPYTYGRVVLSPDGGHVAFDDYDYSASAGSVNSAALIGTRVKVCEVPGG
                     REHFSLPVGNEILFSVAFSPDGRLLVTGDAAGKTCVWDAQTGSMLYSADQAGPIFRFA
                     FSPDSRWLAGADREKVRIWNAVDGQEVLVLRGAPPRSLDGGFNPALAWSRDGQQLASL
                     NWDGSISIWSASPQPISPSQRWNAAGGRVFAWHLAEAQAAVDQAQTQAAQFHLTRLAD
                     EAPPDIVTLLGRARLFLLLGQFDRAAADYAHWSDTGEPDNGTAWLSYARLLIARGDFD
                     GYRRHCARMLDAVAKQINPQMVSCAAQTIGLSPGPPNHAQNVIALLERAIPLDRRNAH
                     IEFALALAHLRAEKFEDAAILLGKVTDREPDWGWRSWPVLAIIERRRGRLEESKRELD
                     KARAWLDQHREAMTAGSNNSLISLDRLDFEAFCREAATLSGPAK"
BASE COUNT         2332 a         3443 c         3697 g         2237 t
ORIGIN      
        1 ccccggagtc ggcgtgcaac gaccagcgtg acccggttgc ctcccaaacc aggcgaggat
       61 aggcggccag gatgctccat cggcctgccc cgctaaaccc tggtccgctc tcgagcaacg
      121 cagacctggg ccaagcgccg atcaccgagg ccaacgaggc ggctggcagg tcgagaacct
      181 ggtgcacaac tgagggaaga acacgggttg acacaacgag cctcaaaccg aggaatcatg
      241 gtgcgcctgg gttgcagatt ttgatcttca tcgatccttg cgatcctttc aaccgtaggg
      301 acgtcgaccc aaggcgagaa ccaagctacg attgcttttt aaaacttcgc ccttccctcc
      361 tgcggaaagg aaccatgatg gcgacacgaa tgagtacctc gatggcagca cggagcaatc
      421 gaggcaggaa ccggctctgc gacgttgtcc cgctggcatt attgacgctg tccatcgtca
      481 cgccaggcgc ctggggacaa gtcagcttac ccgatcaggg gaaagtggtc acctagcttg
      541 aaaaacgggg cgcccaggtc tttcgggacg agacgcaacc tgaccggcca gtaatcagcg
      601 tggatctgga cgagagggag ttcgacgacg cagggctgtc gagcttgaag ggactgacgc
      661 gtctccagtc gttgtcgctt gacggaacca gaatcaccga cgcggggttg gcgaatctta
      721 agggcttgac gagcctcatt gacttatcgc tcgataacac ggaaatcggc gatgccggac
      781 tggcccagct tgaaggattg aaaaacctgc agaagctgtc tatcgaccgt acgagggtca
      841 gcgatgccgg gctggctcac ctcaaaggaa tgaaacggct cgaaagcctc tcgctcgtga
      901 gcacggccgt gacggatgct ggcctggcgc atttgagcgg ccttactcgc ctgcagtggc
      961 tgcttctgca caacacccat atcggcgacg ccggcatcaa acacttgaaa ggcttgaccg
     1021 acctggagtt tctgttcctg cacaacaccc aggtgagtga cgctgggctg gaatatctga
     1081 aggggctcaa gcggcttcaa atcctgaatg tggaaaactc ccgagtgacc gaggccggag
     1141 tgcaacagct ccagaagctg aggccgcagc tcaagatttc cttctgatgg ttttgcacca
     1201 cacgttcgtc acggtcctgg catttgattc gtactcacag agccgtgttt tgtgcggttc
     1261 ggatcgacgg tgcgacgatg cgtgttgcgg aaaaacaacc cgaccgggaa ggccgcgcta
     1321 actgtcgacc gtaagtccgg cacagattga cttatcctct tatatggctc ttaattgcac
     1381 gcattcaggc ttgtttttcc gggggaatgg tttataatat taacggttat tgaaagtccg
     1441 ccgcgtgaac gattccgggg gggctctcaa tgaagatcgg cgctggatct cttggcagcg
     1501 atgccattgg gttcctggcc tcgggagacg caaggcagca aacacggccc ccagggtggg
     1561 ttccccaaaa ggtcgtcctg aagcatctca tgacgcatct cgatcgtgac attttggtgt
     1621 cgcgaaatcg tcgtcgcgtc gcagtcggtc tcggtgtcca ttctgggcac ctccaattga
     1681 agttatcggt gaagtggatc gcccactttg tccggcgggg atagtacgca gatgctccgc
     1741 gccaggcttc gtaccgtaaa ttcttgcagg gagggcgagt tgcctgccga gcccattaca
     1801 acagaggcaa ttgcatcccg agacggctcg gcaggcaact cgcatttccc tagacgtgcc
     1861 ggagagatcg attctgaacg aatggcaacg atattgcgta atgatttcag cacccttccg
     1921 ctcgaccaga gcgggacgca gtagcgtgcg atagagaggg gtatgggcca tgcgaacgat
     1981 gaaagtgagg gctctcgatg gccgctggcg aagctgggct ccatcgtggc tcgcgagtca
     2041 tcggccttcg cgtctggctc tcccccagcg gcctaccgtg tcgcgatcga gtggtccggc
     2101 cgcgcggaca atctcccgcg aagatctcaa gaaacggatc gcgacggcag ttctggcatt
     2161 tcgcggctac gacctgacaa acctcggccg cagtcccgag ctgctggagc accgtgttta
     2221 tgggccggtg gttcgggcgg cactcgaatc agcgtcagca ctctgcggtg acgtcctggg
     2281 ccaaaaggta gacctggcgg cccgggtgct ggcccgtgaa ccgtccacgc tcgaaacttt
     2341 cgtccacgat atcggcacga tcgtctccat ggagctggcc cagatccgtc tgctggaaga
     2401 gatcttcgag atccccgttc cacacgcacg actgagcttt ggccacagca ttggtgagct
     2461 ctccgctctc gttgttggcg gcatgtacac catggagcaa ttgctgccca ttcccctggc
     2521 actcgcgaca gattgtgctg agcttactgc caacacgacg atgggcattc tctcgaccca
     2581 gggaaacaca cttcagattg aagacgtaca gcacctgtgc tcgtcgatca gcagccgtgg
     2641 gcaaggtctg atcggaccct cgacgtatct ctcgccgttc caggtccttt tgctcggcca
     2701 gggaaacacg ctggatttgc tcgagcagga gatgggcgag ttcctgccga ccggagtgac
     2761 attgcggcgc aagccgaacc gctggccccc cttgcatacg ccgctcgtgt gggagcgcaa
     2821 tattcccaac cggacggcga tgatgatgta tcacacggcg ggcggtctgc agaaaccgac
     2881 accaagtgtt ctgtcgtgta cgactgggct cgccaattat gatgcgtgga acagtcgggc
     2941 gatcctggcc gattggaccg atcatcccca gcggctatgg gatgcgctgg aatactcgtt
     3001 ggcatcgggt gccgagctgg tcattcacgt ggggccggat ccgaaactgc ttacgacgtg
     3061 ttttgaccgc ctgagccaca ggatcatgaa gcaattaaag atgcggcacc tcgaccgcct
     3121 ggggtcgagt gtgataccga gtatcggccg caatggctgg ctgacccgta agcttcccgc
     3181 caacgcggtg ctgttgcggg ctccgttcct tgatcatctg gtcctggagg actggttgct
     3241 ggcccaggat gtctgataac atcacgtgcc gtccccgagg ggtcgggggc aagagcagcc
     3301 ttccgtacgt gaatgccgga gggtgaggct cagtgcggcg cctgattctt ccaagggtta
     3361 caaggcccgg ccatgtggtg ctgggagagg aagaggtttt cccatgagta cccaaactcg
     3421 ggagcccgaa acccaaacgt tcgtcgaggc cgctctcaag ctgggcggca agagcgagga
     3481 ggaggctcgg acaaccggca cagtcgaccg ggccgatgac caggtcgacg ctctgttcgc
     3541 cgcgcggtac cagacggcca acagcccggc ccatcgcatc gtctgggatc gcgaggccac
     3601 gctcgactta ttcttgcctc agccgccggc gacctcgccc gccagcgaga agatcatgca
     3661 gcggtcgctg gaggcggtcc gcaaaaaccg cgagcaaggg agtctgtatg ggcctgacgg
     3721 caaagtgtcc gaggcggtgc tgaacgatct atcccaagcc ggttactggg ggctcttgat
     3781 cgctcccgag tacggcggtg gtggtgcttc gtttgcctcg ttcgcccgat tccttaccaa
     3841 gatggccacg atcgagccca acctggccgg cctggcgtcg gttcacggat gcatcggcgc
     3901 ggtcgatccg ttgcgaacat tcggaaattc agagcaaaag gaacggtatc tcccccgctt
     3961 ggccagcggc gaacggctgt cggcctttgc gctcaccgag cccggagccg gctcggactt
     4021 gaccgcgctc aaaacccagg ccgtgctcga cggcgacgac tacgtagtca atggtgagaa
     4081 actgtttatc accaacgccg tcccgggacg cacgatcggc ctggtgtgcc tgatcgacaa
     4141 gcgccccgcg gtgctgattg ttgacttacc cgatcacgag gacgagcatt tccaactggt
     4201 gacctacggc ctgctcgcgc tgcgtcactt gaacaatcat gggctgcgat tccgcgattt
     4261 ccgcgtgccg cgcgagaact tgctccagcc ggctcaaggt gacgggctca cgatcgccta
     4321 tcatggtttg aatctggggc gagtggcgtt gtgcgccacc gccgccgggt cgatgcgcgt
     4381 gatgctcgcc aacatgcttc cctgggccaa gtttcgccga acgtacggcg cggcgattgt
     4441 ctcgcgcgag ctggtcgagc ggcggatcgg ccgaatggcc gcactgatcg tgggttgcga
     4501 tgccatggtc gattggtgct cgtggctctt ggaccagggg ttccggggcg agctcgagtg
     4561 catcatcgcc aagatttttg gcagcgagtc gcagaaagaa gcggccgtcg agctgttcat
     4621 gaaaacgcac ggaggccgcg cgttcttgaa aggtcacttg ttcggcgaca atattcacga
     4681 gtatctggcg ccttgcattt acgaaggtga aggcgagatg ctcggcatgg cgtttttcaa
     4741 gtcgttgatc aaagagcacg gcaaagagtt cttcgagccg atcgggcggg cgatccaagc
     4801 atcgggcatc aaaaagccca acccgatgaa cccggcccac gcctgggcgc tgcggtccgc
     4861 attggtgccc tatgcgaaat ggcggaccgc ccagatggtg acaggaaagc gccgtctcaa
     4921 cttcgatagc ttgtcgcttc caccggcctt ggccgcgcat gccgaatatg cggcggaggc
     4981 tctcgagaac ggccggctcg aaattagtga cgtgatgacc aagcatcagc ttcgactggc
     5041 cgaccgtcaa tgccgcatgg ccgagctgtc gcagcgcctg caagacctgg tggtcatcct
     5101 ggccaccagt ctctgggcga gccgtcagtc gaacgaggtc gtgcaatccg cggccgacat
     5161 cctgtgccag gatttgaccc ggaagattac ctgcaaacgc cccaccgacg cgtatttccg
     5221 tgccgtcacc aagctcggag agacggttgc caatggcggg ttcgaggcca tcgcgggcat
     5281 cgaggccggg gaaatcctca tgccctacaa cgcttgatcg cgcgaaggag agatcatggc
     5341 gaacgctttc cggttcgatg agctcgatgg caagatcggt gtcgtcacct ttgacctccc
     5401 cgacaaaaag gtcaacacgc tgtcgcagga ggtgttgaaa gagctggcgg gcctggtcgc
     5461 tgagctcgag aagcgcaccg acctcgttgg gctgttgttc aagagcggca agcctggcca
     5521 gtttgtagcc ggcgccgact tgaatgagct ggccatgatg agctacgcca agctcgagca
     5581 ggtggccggg ctcaccaaca tgggccatca gctatacaat cggatcagcc gcttgccgtt
     5641 tccgaccgtc gccttgattg acggtggttg cttggggggc ggaaccgagc tggttctgac
     5701 gatggacgag cgcttggcat cgacggcgcc acacacgcag atcagcctgc cggaagtttc
     5761 gatcggcatc ttgcccggct ggggcgggac gcaacgcttg ccccggttga tcggactcaa
     5821 tgcggcgatc gaagtgatct gcgcaggaga aaagctctcg ggccagaaag ccgtgaccgt
     5881 gggctttgcg ttcgacgccg tcccggccga aaagttggtc gaggaagggc gccggcttat
     5941 cgaatacttg cagcagagcg gcgaatggaa atcccagcgc gagcggttgc aagggccgct
     6001 gggactgaat gcggacgagc tcaatttcgc gtttgcggtc gccgaggggg cgatcaaggg
     6061 caaaaccaag ggacattacc ccgccccgct ggttgctttg aaagcgatcc gcgagggctg
     6121 taacaagacg ctcgaagaag ggctcaaagc cgagcagaac gggtttggcg agctcgccgg
     6181 ctcgccgatc gcggccaacc tgatcgggat cttcttcttg aaaaaccgcc ttgcgcgcga
     6241 ccccggcgtg agtgatccgt cggtcaagcc tcggccagtg cacagcgttg gcgttctggg
     6301 cgccggcttt atgggggcgg ggatcgccgc ggcgaacgcg cgctctgcta tccgcacctc
     6361 gatggtcgat gtcgatgacg ctcgaatagc cgacggcctc aagcgcgcct cggacgtggt
     6421 gatgagccgg atcaagatcg gccgcgccac cccgcaagat atggcgagca tgcttgctct
     6481 cttgaacacg tcgacatcaa ccaagatctt cgccgatagc gacatcgtga tcgaggcgat
     6541 caccgaaaac gaggccgtca agaaagaggt ctatcgcggt ctggccggtg tgctcaagga
     6601 cgatgccatt ttggcgagca acacctcgac gatctcgatc acgcgcatgg ccgaatctgc
     6661 cccacatccg gagcgattca ttggcatgca ctttttcaat cccgtcgatc ggatggagct
     6721 ggtcgaggtg atccggggcg agaagacgag cgacgagacg gtggccacgg tcgtcgcgct
     6781 ggcgaaacgc atccggaaaa cgccgatcgt cgtcaaggat tgcgcgggct tcctggtgaa
     6841 tcgcgtgctg ttcccctaca tgaacgagtc cttgatactg ttgcaagacg gggcgtcggt
     6901 cgaagcgatc gaccgggcgg ccacgaagtt cggcatgccg atgggtccca tgatcctgac
     6961 ggacttggta ggcctcgata cggcggtata cgccgggaaa gtggtttcga aagcgtatcc
     7021 ggaccggacg gtaacatcgc cgatcctgga agagatgttg aaggccagtc caggcgacaa
     7081 gaaatcgctg atgaaattct ggctgtcgga caagaaggcg aaaacccagc ccaacccagc
     7141 cgccttggcg atcatcgcca agtaccgcac gggtgatcgc gcgatcgaat cggacgagat
     7201 cacggatcgg ctgttcctgc cgatgctctt ggaggcgaca cgggttctgg aagaagggat
     7261 cgtccgcgag ccgggggacg tcgatatggg gttgattctg ggaattggtt tcccgccgtt
     7321 tcggggtggc attctccgct ggtgcgatag tttgggcgcg gccgcggtcg tcgacaaact
     7381 cgcgaagtat gcacacctgg gcaagcgatt cgagccaacc gagtcgttgc tcaagctggc
     7441 gaagacaggt gagacgtttt atccgcgccc gaaattggtc aaaggctgac tttcacaact
     7501 cacgcactcg tcaggctaga aagcctgacc tacgtaggac tctggctctc tgaggattcc
     7561 ttcgatgaaa aacgccgtgg tcatcgacgc cgtccgcacc cccgtgggcc gggcgtcatc
     7621 agaccgaggt tactaccgcg acgtccgttc cgaggacttg tccgcccatg ttatccaggc
     7681 gctcctcaag cggacgggcg ttgcgcccaa agagattgaa gacgtgcgct ggggctgcgt
     7741 gatgcagcaa ggcgaacagg gtttcgacgt cgcccgcatt gcggcgatgg tcgccggcct
     7801 tccgccggag actggcggtg tcacgatcaa tcgcaactgc gcctccagct tgcaggcgat
     7861 caacgactcg gcgatgagca tcgccgctgg ttgcgaggat gtgcagattg ccggtggtgt
     7921 cgagcacatg gatcatgtgc cgatgacgaa agactacaac ccgtgtccct cgctgtttcg
     7981 gcatcacagc gaagcgatca tgaacatggg attgaccgcc gagtatctgg ctgccaagta
     8041 ccggattccg cgtggcaagc aagacgagtt tgccctgcgc agccaccagc ttgcgaccaa
     8101 ggcgaccgat gcgggcgagt tcaagaatga aatcataccg acatgggggc gcgatgatgc
     8161 cggtcgtaag gtgctgctaa caacggatca gtgcatccgc cgcgacagtt cggccgagtc
     8221 gctggcggcg ttaccccccg ccttcaaccc ggaaggggga tcggtgacgg cgggcaacag
     8281 ctcacccatt aacgtcggtg cggcggctct cttggtgatg tcggaagaga aagcgcggga
     8341 actcggattg aaaccgatcg cgcggatccg ggccatggcc gttgctggag tgaatccaga
     8401 ggaaatgggc atcgggccgg tccctgcgac tcaaaaagcg ctcaagcggg ctgggctcac
     8461 tctggatcag atcgattgct tcgagttgaa tgaagctttc gccgttcagg tcttgtccgt
     8521 gctcaagtta cttggactcg gcgaggaaaa ggtcaacaaa cgcggtgggg cgatcgccat
     8581 cggccatcct ctcggggcca gcggcgcgcg gatcgcaacc acccttttgc acaggatgcg
     8641 cgatgaagga gccaagctgg ggttggcgtc gatgtgtatc gggcaagggc aaggagtaac
     8701 gaccatcttt gaggcgtgct gaatgattcg ccatccgcta ggactgcgtc tcgactccga
     8761 tcggtccgtg cgcgatcaga tctatgagtc agcgcggatg ggtgcccgcg gcgtggttat
     8821 tgacgcgagc gatgatgtgg cgccgcaccg gctaggagcg actggccggc gcgagctgcg
     8881 ccaaatcctc cgcacggtgg agctgtcgct tgtggctctc tcgctcccga cccgcaggcc
     8941 cttcgactcg gccgaccagc tcgatgaccg gatgcggcgg gcggatgcgg cttttgccat
     9001 ggcctacgag ctgggcacga caatcgtgtt ggcgcgcgcg ggcgccgtcc caccgcccga
     9061 tgacaatgcc cgtctggagg tgtttgccgg cacactgggt gaattgggcc gccgcgccga
     9121 gcaccacggg gtccggctcg cgctggaaac cggatctgag cccggtcaga agctcaagga
     9181 gtttctcgac gctttgggtt caccaggcct ggccgccagc atcgatcccg ccagcatcct
     9241 ccaagcgggt atcgatccca ttgtcaccgt gcgagagctg gcatcctggg ttgtgcacgc
     9301 ttacgccaaa gacgcgaccg gctcgagcgg cgtcaagtcc gtcaatcccc gagggtttgg
     9361 ctatcctcct ggcgccctcg attgggaaga gtatctgggc tcgctcgagg agatcggcta
     9421 tcacggcttc ttgaccgtct ggccggcgcc tgggctgccc gcggcggccc aattcaccgc
     9481 ggtatccgat cggctcaagc aagttttgtg acacctgcag ccgtcgtctc atttcgccgg
     9541 gcccgagagc gttgctgcct cacggcagaa tgcctcaaaa tcaagccgat ccagtgagat
     9601 gagcgaatta ttcgagcccg cagtcatggc ttcacgatgc tgatcgagcc acgctcgagc
     9661 cttgtccagt tcacgtttcg attcctccag acgacctcgc cgccgctcga tgatcgccag
     9721 cacgggccac gagcgccatc cccagtcggg ttcgcggtcc gttactttcc ctaaaagaat
     9781 ggcggcatcc tcgaattttt cggctctcaa gtgggcgagt gcaagagcga attcgatgtg
     9841 ggcattacgc cggtcgaggg ggatagctcg ttccagaagc gcgatcacgt tctgggcatg
     9901 gttgggcgga ccaggcgaga gcccaatcgt ctgggcagca caggacacca tctgggggtt
     9961 gatttgcttt gcgacggcgt cgagcatgcg ggcacaatgg cggcgatatc catcgaagtc
    10021 accgcgcgcg atcagcaagc gggcgtagct cagccaggcg gtgccgttgt ccggctctcc
    10081 cgtgtcggac cagtgagcgt agtcggcggc ggctcggtca aattgtccca ggagcagaaa
    10141 caaccgagcg cgtccaagaa gagtgacgat gtcgggaggt gcttcgtccg ccaacctggt
    10201 gaggtggaat tgggccgcct gagtctgtgc ttggtcgacg gcagcttgag cctcggcgag
    10261 atgccaggcg aagacacgtc cgccggctgc attccaccgt tgagacgggc tgataggctg
    10321 gggagaagcg ctccagatgc tgatgctgcc atcccagttc aaggacgcga gttgctgtcc
    10381 gtcccggctc caggcgagcg caggattgaa acctccgtcg agtgaacgag gcggggcgcc
    10441 tcgcaagacc aagacttcct ggccgtcgac ggcgttccaa atccgcactt tctcgcgatc
    10501 cgcccccgcc aaccaccggc tatccgggct gaaggcgaat cggaagatcg gtcccgcttg
    10561 gtcggccgaa tagagcatcg aaccggtttg tgcatcccag acgcacgttt ttccagccgc
    10621 gtcaccggtg accagtagtc gaccgtcggg gctaaaggcg acggagaaaa gaatctcgtt
    10681 gccgaccggc aaactgaaat gttcgcgacc accgggcact tcgcagacct tgactcgagt
    10741 cccgattaat gcggccgagt tgactgagcc cgcactggcc gaatagtcgt aatcgtcaaa
    10801 cgcaacgtga cctccatcgg gactcaacac cactcggccg tatgtgtacg gggtgggtgc
    10861 gtttgagggc ttaaaggaag cgagaatctg tccgctggtt gcgtcccaga ccttcacttc
    10921 ccgtgaacgc acctcgtttt tcgcatagag gccggacgcg gctgcacgac tgccatcgcg
    10981 gctcaaagag acaaacgtgg ccggcgccga caggcctcgg agcgcgatca gctcagtacc
    11041 cgattgcggg tcgcagactt tcacgaacaa ccggtttgat gaaacggctg cgaagcgccg
    11101 accttccagc gagaattcgg ccggggccgc gggagtgagc cattgcatcg tcatgtcaac
    11161 ttgggcgccg acccttgtcg atccactggc gatctcacgc acttgcagtc ctcctagatt
    11221 cgtcaccagg ctcaactttc tgccgtcgtc aacaaaagcg aacgctggag aatggcagtc
    11281 cgttaaagtg agattctcct gagggtgggt cagatcccag agcttgactt ggccaatatg
    11341 acgtccaccg gagagcaggc accacccgtc gggatgaaaa gccacgcaac cgaccagggc
    11401 ggagtggcca cgcagtacgg cgcgttcagt gccggtctgg acgtcccaga gccggatcgt
    11461 cgaatcgacg ccggcagtcg cgacctgtcg gccgtcggga ctgaaggcca ctcccggcac
    11521 agacccggcg tggccgctga gctgatggag aggccggccg gttgacgcgt cccagatccg
    11581 tggcgggcgt gtcgcgacga ccacatattt tccgtcgggg ctaaaagcca gctcagggcc
    11641 ctcaccgtcg aagaaacgct caattcgggc cgcgtcttca ccagggccgt gcgcgagcga
    11701 gacaatttc
//
LOCUS       DATAQL010000190         7090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1852696, whole genome shotgun sequence.
ACCESSION   DATAQL010000190 DATAQL010000000
VERSION     DATAQL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852696"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VHS97_03160"
     CDS             <1..188
                     /locus_tag="VHS97_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447222.1"
                     /translation="TVAPGDNLQFTVSQVTRGSLDYARKGDYAFLMPRQLNPKGLVLS
                     QLVLYKSIPFSQFSLTR"
     gene            complement(295..1950)
                     /locus_tag="VHS97_03165"
     CDS             complement(295..1950)
                     /locus_tag="VHS97_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX3447223.1"
                     /translation="MPRSWPAIARVRRIGEVWAQFWRGEYDRSTLRSVPSWGMSFLIH
                     ALMLLLLAFVFHLRRSGESLALSIQPAIVDTQLGDVTSLVDATRAGDPFTTNDSPDPP
                     SLGLAPTDPDIKLVGQPQIASLSKFAPAMASPMLEASVKPGVSSFAKSRTKGLALEGM
                     MRLPEFAEAVSAPFSGRDGLTRAKLLRREGGTAKSEKSVEDGLAWIARHQRADGSWSL
                     NFQDQCQAEPCPHSLASIDSDTAATGMALLPLLGAGHIHTVKTRYQDTVRRGLEWLTA
                     HQGPDGDLFVGPPGMAYLYSHAIATMALCEAYGLSRDPSLEPAARRAIAFICNAQDPV
                     SGGWRYSPGQTGDTSVFGWNIFALRSGHLAGLPVPRKVLKSCTNYLDMAAVDKQRVVY
                     SYQPGRQRGDGLDAVMTTEALLSRQLLGWPRDFPPLIKGVGMISAHLQQSSDRNIYYW
                     YYATQLLHNMGGDKWQRWNLKIREGLISMQVKDNSCAQGSWDPFLPQPDLWGRMAGRL
                     YVTSLSILTLEVYYRYLPMYRSYDEDQAKPDSAMKEKDDDKPK"
     gene            complement(2120..4786)
                     /locus_tag="VHS97_03170"
     CDS             complement(2120..4786)
                     /locus_tag="VHS97_03170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3447224.1"
                     /translation="MPDSNPLTNFRFEKTKLYNGLDVIIRRQAQLPIVAINVWYHVGS
                     KNEERYQRGFTHLFEHLMFEGSAHYPGDFFKHLQPLGANINGSTSSDRTNYFVDLPTA
                     HTEQAVAMESDRMANLLGALDQTKLRVQKDVVKNEYRQNYANRPYGMVGALIAETLYP
                     PEHPYSWMTIGVMEDLERATLDDVSAFFRRFYVPSNASLALVGDLDLDHALAMVERYF
                     GPIAGGSAAPRPWVPALGLKDTAEIVLRDRVELERLYVVWPTVPHFHADDAALMLLGD
                     VLARGRSSRLHKKLVIDEQIAQDVTAYQAGRELDGSFGIIVTLRPSRSINQARRLVES
                     ELLTIANEGVTDEELRRVRTMRIASFFFALEHVGGFGGVADRLNAYNVFRGDPSLITS
                     DVERFESVAGTKLGGVAAAYLADRPRVNLSVLGQKKAVSKTPLDRSLIPAAPAPSHYR
                     PPAPTVIQLNCGIPLWVLPRSDLPTVAGAIVLTAGAGLQEPGQAGLAQLTSVMLDEGT
                     KTRSAESIALAVEAMGATIGASSGWDGSYVSFRCLKADLPRVLDLTVDILLNPTFPEG
                     EWGRVRGQTLAALRAESDNAESSAYRAFLAAIYADDHPYRYPLAGTEKTVSGFSPDQL
                     ADFHARHLVPGRAAIVVAGDVDPEALADEFCRRLPPLTRKGEGFPAAAPPIKRSLRRV
                     LLLDRPGAPQAVLRVGHLGLARSDPAFEHTVVLNQILGGQFSSRLNSKLREERGLTYG
                     VRSHFDCRGQAGPFSVTTSVQADRLAIALNDIRLELEALTSDRPPNQVELDNARRSLV
                     EGHARQFETPSALVNRFAGLIIHGLPPDHEAGFADRLASIDVASLQVQARERIDPGSL
                     VAIVVADAESVIDELKGIGWADVETIDGPGVP"
     gene            5251..6426
                     /locus_tag="VHS97_03175"
     CDS             5251..6426
                     /locus_tag="VHS97_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX3447225.1"
                     /translation="MNWDDIIIDAAATDTGMRRSNNQDSYMAVRASHEESWRQRGHVF
                     LVADGMGAHAVGELASKMACDLIPHTYMKARTGTPSEAIVKSFREVSAQIHSRASANR
                     DFQGMGTTCSALLLLPTGALVAHVGDSRVYRIRKGRIDQLSFDHSLAWELVRRNHLTS
                     EQASLSVPKNVITRSLGPEIEIEVDIEGPLAVELGDVYLLCSDGLSGPVDDDEMGAIA
                     ANFHPNDACRYLVSLANLRGGLDNITVVIVRIGEWVDPDSGETLNRVPTPKHVRDRSG
                     REGWRSRLSELVTGRRRQGSLALVEDHPYRTAECNINETLVDRFDELTRNIQEQAIAQ
                     AWSLDWAALATLRRQATDARTAKNLWVSLRKAGELIFMLGQAARFYRKNAGPATPVQ"
     gene            6475..>7090
                     /locus_tag="VHS97_03180"
     CDS             6475..>7090
                     /locus_tag="VHS97_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX3447226.1"
                     /translation="MSSSDSFTRLSTSCARGPARVESASPPLVSPLELSAVYCPADLD
                     HVDALSEGRSKGFIYARDGHPNAAQLAEKLAHLEGGDGALICASGMGAIASVFLSLLS
                     QNDHALISDGVYGKTTSLVTRLLPRWGIDHDIFDAAAGPATLKSLLKPETRLIYTETA
                     SNPLLKVADLDSMAAVARQAGVPLIVDNTFTPVICRPIDHGASLV"
BASE COUNT         1410 a         2148 c         2144 g         1388 t
ORIGIN      
        1 cgaccgtcgc ccccggcgac aacctgcaat ttaccgtcag tcaggtcaca cgcggctcac
       61 tcgactatgc ccgtaaggga gactacgcct tcctgatgcc caggcagctc aatccgaagg
      121 ggcttgtgtt atcgcagctc gtcctttaca agtcgattcc cttcagccag tttagcctaa
      181 cccgttagta ggtcagtctt tccggggtga cgtcaggcta gaaagcttga cctacggtgc
      241 cgaagtcgcc gacatcggca caatgctcgg gccatgactg tccccagatc cggatcactt
      301 cggtttgtcg tcgtcttttt ccttcatcgc gctgtcgggc ttggcttggt cttcgtcata
      361 agagcgatac atcggcaaat accggtagta aacctcgagt gtgaggatcg acaacgaagt
      421 tacatagagg cggccggcca tgcgccccca aagatccggt tggggcagaa acggatccca
      481 gcttccttga gcgcacgagt tatctttcac ctgcatgctg atcaagcctt cgcggatctt
      541 gagattccag cgctgccact tatcgccccc catgttgtgc aagagctgag tcgcgtagta
      601 ccagtagtag atattccggt cgctcgattg ctgaaggtgg gcggagatca tccccacccc
      661 tttgatcagg ggcgggaaat cgcgcggcca gcccagaagc tgccggctta gcagggcttc
      721 ggtcgtcatg accgcgtcga ggccatcgcc gcgctgccgg ccgggttgat acgaatagac
      781 tacccgttgc ttatcaaccg ccgccatgtc cagataattt gtgcacgact tcaagacttt
      841 gcgaggtacg ggaagtccgg cgagatggcc gctgcgcagt gcgaaaatat tccagccgaa
      901 aaccgacgta tcgccagtct ggccgggcga atagcgccat ccgccgctca cgggatcctg
      961 ggcattgcag ataaacgcga tggcccggcg agcggctggc tccaagctgg ggtcgcgcga
     1021 caagccatag gcctcgcaca gggccatcgt ggcaatcgcg tgactataaa gataagccat
     1081 gccaggcggc ccgacgaaaa ggtcgccatc gggcccttgg tgagcggtca gccattcgag
     1141 tccgcgccgg actgtgtcct ggtagcgggt cttgaccgta tggatgtggc cggcgcccaa
     1201 gagaggcaac agggccatcc cggttgcggc agtgtcggaa tcgatgctcg cgagagaatg
     1261 tgggcatggc tcggcctgac actggtcctg gaaattgagg ctccatgacc catcggctcg
     1321 ctggtgtcgc gcgatccatg ccagtccgtc ctcgaccgat ttctcggact tcgctgttcc
     1381 tccttcgcga cgaagcaact tggcgcgcgt cagaccatcg cggcccgaaa acggggccga
     1441 cacggcctcc gcgaactccg gcagccgcat catcccttcg agcgcgagcc ccttagtacg
     1501 cgacttcgca aacgatgaga cgcccggctt tacgctcgcc tccaacattg ggctcgccat
     1561 cgcgggagca aacttggaca gcgacgcgat ctgaggctgg ccgaccagct tgatatcagg
     1621 gtcggtcggc gccagtccga gcgacggcgg gtcgggacta tcgttggtcg tgaaggggtc
     1681 acccgcgcga gtcgcatcga ccagagacgt gacatcgcca agctgtgtat cgacgatcgc
     1741 cggctggatc gataacgcga gcgactcacc gctgcgccga aggtgaaaca cgaatgccag
     1801 cagcaaaagc atgagtgcgt ggatgaggaa gctcatgccc caggatggga ccgatcgaag
     1861 ggtacttctg tcgtactcgc cacgccagaa ctgagcccac acttcaccga tccgccgcac
     1921 tctggcaatc gctggccagc tcctgggcat gagaggtccc tcgcggcatc aaagaaaaac
     1981 ggattaccga agaaagggcc aacgaccatt ataggactcg caaagcatag tgccagcacc
     2041 gacgcgccgg cgcgtgtatt tttgagattg gcttgaaccg attgcggcgg cgctacgcta
     2101 gattgcgact ggctcccact cacggcactc ctggcccatc gatcgtctcc acgtcggccc
     2161 aaccgatgcc tttcaattcg tcgatcacac tctcggcatc ggccacaacg atcgcaacca
     2221 gcgatccggg gtcaatccgc tctcgagcct ggacttgcaa cgacgcgaca tcaatgctcg
     2281 cgagccgatc ggcaaaccct gcctcgtggt cgggcggaag accgtggatg atcagccccg
     2341 cgaatcggtt cacgagcgcc gagggagttt caaattggcg ggcatggccc tcaacaagag
     2401 atcggcgcgc gttatcaagc tcgacttgat tgggaggccg atcgctcgtg agcgcctcga
     2461 gctcgagtcg gatatcgttc agggcgatgg cgagccgatc ggcctggacc gacgtggtga
     2521 cggaaaatgg acccgcctgc ccgcgacagt cgaaatggct gcggacgcca taggttaacc
     2581 ccctctcttc gcgcaacttg gagttgagcc gggacgagaa ttgacctccc aagatctggt
     2641 tcaaaacgac ggtgtgctcg aaggctgggt cggagcgagc aagtccgaga tggccgacgc
     2701 gcaagaccgc ttgcggcgca cctggccggt cgagtaggag cacgcggcga agggaccgct
     2761 ttatcggcgg ggcggccgcc gggaatccct ctccctttcg tgtcaatggc ggcagccgcc
     2821 ggcagaactc gtcggcgagc gcttccgggt ccacgtcgcc ggcaacgaca atggccgctc
     2881 ggccagggac aaggtgtcgc gcgtggaagt ctgccagttg atcgggactg aatcccgaca
     2941 cggttttttc ggttcccgcg agcgggtatc ggtagggatg atcgtcggcg tagatcgccg
     3001 ccaggaacgc ccggtaggcg ctcgactccg cgttgtcact ttcggccctc aaggcggcga
     3061 gcgtctggcc gcggacccgc ccccactctc cttccggaaa tgtcgggttc aagaggatgt
     3121 cgactgtcag atccaggacc cttgggagat ctgccttcaa acacctgaaa gacacgtacg
     3181 atccgtccca gccgctgctc gccccgatgg tggcgcccat cgcctcaacg gccagggcga
     3241 tcgattccgc cgagcgcgtc ttggttcctt cgtccagcat gacggacgtc aactgagcga
     3301 gcccagcttg ccccggctcc tgcaggcccg cccccgccgt gaggacgatc gcgcccgcca
     3361 cggtcggcaa atcgctccgc ggtaagaccc aaagcggaat tccgcagttt agctggatga
     3421 ctgtcggggc cggcgggcga tagtgcgaag gcgccggtgc ggcgggaatt agggaacgat
     3481 cgagcggtgt cttgctgacg gctttcttct ggccaagcac cgacaaattc acccggggcc
     3541 ggtcggccaa atacgccgct gcgacaccgc ccagctttgt gcccgcaacg ctttcgaacc
     3601 gctctacatc cgaagtgatc agcgacggat caccacgaaa gacgttatat gcgttcaagc
     3661 gatcggcgac cccaccgaag cctccaacgt gttcgagcgc gaaaaagaaa ctggcgatcc
     3721 tcatcgtgcg gacacgccgt agttcctcgt cggtcacgcc ttcgttcgcg attgtcaaaa
     3781 gctctgactc caccaggcgt cgagcctgat ttatcgatcg cgagggccgc aaagtaacaa
     3841 tgatcccgaa cgacccatcg agctctctgc cggcttgata cgcagtgacg tcctgggcga
     3901 tctgctcgtc gatcactagc ttcttgtgca gacggcttga tctcccccgg gccagcacgt
     3961 cgcccaggag catgagggcc gcgtcatcgg cgtggaaatg aggaacggtc ggccagacga
     4021 catagagccg ctcgagctcg acgcgatcgc ggagaacgat ctcggccgtg tctttcagtc
     4081 caagcgcggg cacccatggt cgtggtgccg ccgagccgcc tgcaatcggc ccgaaatagc
     4141 gctccaccat ggcaagtgcg tgatctagat cgagatctcc cacgagcgcg aggctcgcgt
     4201 tgctggggac atagaaccgg cgaaagaatg ccgagacgtc atccagagtc gcccgctcca
     4261 ggtcttccat taccccgatc gtcatccagc tatatggatg ctcaggcgga taaagcgttt
     4321 cggcgatcaa agcgccgacc atcccataag gtcggttggc gtagttctgg cggtactcgt
     4381 tcttgacgac gtctttctga acgcgaagtt tggtctggtc gagtgcgccc aacaagttgg
     4441 ccatgcgatc cgattccatg gcgaccgcct gttcagtatg ggcggtcggc aggtcgacga
     4501 agtaattggt tctgtcagat gatgtcgatc cattgatgtt tgctcccagt ggttgcaggt
     4561 gtttgaaaaa gtcccctggg taatgagcgg agccctcaaa catcaagtgc tcaaagagat
     4621 gggtaaaacc tcgctgatag cgctcttcat tcttcgagcc gacgtggtac cagacgttta
     4681 tcgccacgat cggaagttgt gcctgcctac gaatgatgac gtcaaggccg ttgtaaagtt
     4741 tcgtcttctc aaaccgaaag tttgtcagag gattcgaatc gggcataacg gagggccatc
     4801 tcgcaacggg gtgtggaatg gttttttggg ggtcacaagc ggatcagggt tcatgtacta
     4861 ttaggaacga tcgatcgacg tgaaaccagc ccgagttttc cagggcggtt gcctgggcac
     4921 gccggacttc tttcggatct aggttttcgt gcgagtgcca agggtggcat tgaaggcggt
     4981 ggcgaggctg ggtggaataa ttccgagcga aaagctcaca gttgcggcgg ttggacgata
     5041 caatttcgga gggagaccgc gtgcttctgg atcacgcgcg acgggccgga cctagtgaac
     5101 aactccgaat tgtggggttc agaatagtcc tgaagacctg gtgatcccgg ggccgattct
     5161 atatatcgtg tggctctttg gcgaacgcgg gcgatcggcc gcctgaaatc gtatcgtcag
     5221 tgacccctga gatgacaaca aggtctcgca gtgaactggg acgacatcat catcgacgcc
     5281 gccgcgaccg acacgggcat gcgtcgttcc aataatcaag actcgtacat ggcggtccgc
     5341 gcgtctcacg aggagtcttg gcggcagcgg ggacacgtct tcctggtcgc ggatggaatg
     5401 ggagctcacg ccgtcggtga gctcgcgagc aagatggcgt gtgacctgat cccgcatacc
     5461 tacatgaagg ctcggacggg caccccgtcc gaagcgatcg tcaagtcctt tcgcgaggtg
     5521 agcgcgcaga tccatagccg cgcctcagcc aatcgcgact ttcaagggat gggtacgacc
     5581 tgttcggcct tgctcttgtt acccacgggt gctttggtcg ctcatgtagg cgattcacga
     5641 gtctatcgca tccggaaagg ccggatcgat caactgtctt tcgatcacag cctcgcctgg
     5701 gagctggtgc ggcgcaacca cctcacctcc gagcaggcca gcctgtctgt tcctaaaaac
     5761 gtgatcacgc gcagcctggg gccggaaatc gaaatcgagg tcgacatcga aggtcccttg
     5821 gccgtcgagc ttggcgacgt ctaccttctc tgctccgacg gtctctccgg tcccgtcgac
     5881 gacgacgaga tgggcgcgat cgccgcaaac tttcacccga acgatgcttg ccgctacctg
     5941 gtcagtctcg caaacctgcg ggggggactc gacaacatca ccgttgtgat cgttcgaatc
     6001 ggcgagtggg tcgaccctga ttcaggggaa acgctcaatc gggtcccaac tccgaaacat
     6061 gttcgagacc gttcgggacg cgaaggctgg cgatcgcgac tctccgagct ggtcacgggg
     6121 cggcgacgcc aaggttcgct agccctggtc gaagatcacc cctatcgaac ggccgagtgc
     6181 aacatcaacg agaccctggt tgatcggttt gatgaactta cccgcaacat ccaggagcaa
     6241 gcgatcgcac aagcgtggtc gctcgattgg gccgcgctgg cgactttgag gcgccaggcc
     6301 accgatgcgc gtacggccaa gaacctttgg gtttcgctcc ggaaagcggg cgaactcatc
     6361 tttatgctgg gccaagccgc ccggttttac cgcaagaatg cggggccagc gacccctgta
     6421 caatgatctc cttgtcgtca ccgcgccgcc atgccccctg aggcatcgaa caccatgtca
     6481 tcgagcgact cgtttacccg cctgtccact tcgtgcgccc gtggtcccgc gcgggtcgag
     6541 tcggcgagtc cgcctctggt ttcaccgctc gaattgagtg cggtatattg tccggctgat
     6601 ctcgatcatg tcgatgcgct gagcgagggt cgatccaagg gattcatcta cgcgcgcgac
     6661 ggtcatccca acgcggcgca gcttgccgaa aagctggccc acttggaagg cggcgacggg
     6721 gcgctgattt gtgcgtccgg catgggagca atcgcttcgg ttttcctctc gctcttgagc
     6781 cagaatgatc acgccttgat atccgatgga gtttacggca agacgacatc actcgtgacc
     6841 aggctcttgc cgcgctgggg catcgatcac gacatctttg acgcggccgc cggcccagcg
     6901 acactcaagt cactcttgaa gccggagacc cggttgattt acaccgagac agcctcgaac
     6961 ccgctgctca aggtggccga tctggactcc atggccgcgg tcgcgcggca agccggtgtt
     7021 ccgttgatcg tcgataacac cttcacgccg gtgatctgcc ggccgatcga tcatggcgcc
     7081 agtctggtcg
//
LOCUS       DATAQL010000191         3895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5059375, whole genome shotgun sequence.
ACCESSION   DATAQL010000191 DATAQL010000000
VERSION     DATAQL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5059375"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VHS97_03185"
     CDS             complement(<1..264)
                     /locus_tag="VHS97_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3447227.1"
                     /translation="MSRTNSVAGAFVVGASRETSGASQADRQPTPARKIKVIVTGGHP
                     GDPEYGCGGTIARYAELGHEVVLLYLNRGEWPPRDPGVSRVAEA"
     gene            complement(471..1094)
                     /locus_tag="VHS97_03190"
     CDS             complement(471..1094)
                     /locus_tag="VHS97_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEX3447228.1"
                     /translation="MSTLALGATAFTTRGLFADELVRTPKQTEGPFYPNKLPLDTDND
                     LLVINDGITPAVGEVTYLTGRILDAKGDPVRSALVEIWQVDNNGAYIHTKDPHGENHD
                     KNFQGFGRFLTSSTGEYYFRTIKPVLYTGRTRHIHFKIKRGSKELLTTQCYVKGEPAN
                     ERDGIYRSIRDPKARESVTVDFTPLPGSRIGELAAMFDVVLGFTPEG"
     gene            1518..1985
                     /gene="ruvX"
                     /locus_tag="VHS97_03195"
     CDS             1518..1985
                     /gene="ruvX"
                     /locus_tag="VHS97_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX3447229.1"
                     /translation="MTRILGLDFGTRRIGAAISDPGRTIAFPLEIYERRGLYLDERHY
                     RELIEENDVDRIVVGLPLHTSGREGELAARARAFGEWLGGVSGRPVIYFDERYTTVEA
                     ENRLIDLGMTRQKRKALRDKLAAQIMLQSYLEAGCPETEAAGVPLADPDEVEL"
     gene            1982..2863
                     /locus_tag="VHS97_03200"
     CDS             1982..2863
                     /locus_tag="VHS97_03200"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3447230.1"
                     /translation="MTTLIIGCGYLGQRLGTILYEQGERVFGTVRSASRASLLAGLGI
                     EPVIANVLEPDSLRELPEANGVFYCVGFDRAAGSSMRTVYVDGLQNVIDRLPRSVMRF
                     VYASSTGVYGQTDGGWVDESSPTSPQHESGKVCLEAEDRVRAWADSGNRSASAVVLRF
                     AGLYGPGRMVRQSILERGEPIPGDPYKFLNLIHIDDAARAAAAALTTTQPHRLYLVCD
                     DRPVMRKEYYAQMATLLGTPEPRFEVPGPDSPEAARDATNKRINNHRLKSSLHLTLIH
                     PDITTGLFASLHTSTHS"
     gene            complement(3047..3241)
                     /locus_tag="VHS97_03205"
     CDS             complement(3047..3241)
                     /locus_tag="VHS97_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447231.1"
                     /translation="MIREAVALVEKEGMHLEIHLTASSMRALAFASASTSDPNPATTT
                     HKKPARLARNGFGINGGSEL"
     gene            3273..>3895
                     /locus_tag="VHS97_03210"
     CDS             3273..>3895
                     /locus_tag="VHS97_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447232.1"
                     /translation="MFSSAGFETGGFAAASRFTGPIADRGSLAQVKEAAQGRVRRGLD
                     ELNSALRSIPPDSPDRPFMTFQIHATMGLLSMYDGKFGEAAGWFDTALAESADNDVPP
                     GLRANFEAMLGVIHLRRGETENCLECRGPSSCIFPIDSEAVHQFPSGSREAIRHFQRY
                     LDKRPDDLGVRWLLNIAYMTLGEYPDKVPQNQLIGIEPLHSRLDVGR"
BASE COUNT          734 a         1176 c         1213 g          772 t
ORIGIN      
        1 ggcctcggca actcgagaga caccagggtc gcgcggcggc cactcacctc tgttgagata
       61 aagaagaacg acctcatgac caagctcggc gtaacgggcg atcgtgcctc cacagccata
      121 ttcgggatca cccggatggc cacccgtgac gatcactttg atcttgcgcg cgggcgttgg
      181 ctgccggtcg gcttgggatg caccagaagt ctcccgcgat gcccctacca cgaaagcacc
      241 tgcgacggaa tttgtccgac tcaacagttc tcgtcgtgaa agcggcttca tcattgttgc
      301 gttctatcta cagcttcgag ccgggggaag gggcgctcag ttagccgcgc gcctggtggg
      361 acagtgggcg cgccttaacg cccccgtcgc tcattcggtc ggcaacggta gtagatgatc
      421 cgagtcaatc gccaaatgtc gcgaacgacg gccgcgtgcg gcgaggctgt ctaaccctcg
      481 ggagtgaagc cgaggacaac gtcaaacatg gcggccagct cgccgattcg cgaaccaggc
      541 agcggggtaa agtcgacggt aaccgactca cgtgccttgg ggtcgcggat gctgcggtag
      601 atgccgtcgc gctcgttagc cggctctccc ttcacatagc actgggtcgt gagcagctct
      661 ttggagccgc gcttgatctt gaaatggatg tgccgggtcc ggccggtgta gagcaccggc
      721 ttgatcgtgc gaaaataata ttcgccggtc gaactggtca ggaaccggcc gaacccctgg
      781 aagttcttgt cgtggttctc gccgtgggga tccttggtat ggatgtaggc tccgttgttg
      841 tccacctgcc atatttcgac caatgcgctt cgcaccgggt ctcccttggc gtccaagatc
      901 cggccggtca gataggtcac ctcgccgacg gcgggtgtga tgccgtcgtt gatcacgagc
      961 aggtcgttgt cggtatcgag cggcagcttg ttgggataaa acggcccctc ggtttgtttg
     1021 ggcgtacgca ccagctcgtc ggcaaacagg ccgcgggttg tgaacgcggt cgctcccagc
     1081 gcgagggtgc tcaaaaaatc tcgacggcta cggggtagaa taatccggct catcggggaa
     1141 cccccctctc gcgcggaaga cagtggtggt aaccggtcta cagcgtaatc ccgacaatga
     1201 ccccacgccg gatacgcggc tcggtcgacc actgtcgggc atcaggagtt gatcttatca
     1261 caacggttgg cggcggctag tgatctgcgg cggtcttgaa tcacgttgag gacggcttca
     1321 cttgggcccg ggaagcaagc tgaacgcgcg cgacctggat aagcttggcc gatggctcgg
     1381 ccgggtccgg aggcgacacc attgccatct cgaaatggag aactccgggc gaaattgccg
     1441 atggttcgcg atacgatgaa gatcgcaggc cgctcggaaa gtgatagcct cgttttttcg
     1501 tactcggatg tcccccaatg actcgcatcc tcggtctcga tttcggcacc cggcgtatcg
     1561 gtgcggccat cagcgacccc ggccgcacga tcgcctttcc cctcgagatc tacgagcggc
     1621 gcgggctata tctcgatgag cgtcattatc gtgagctcat cgaggaaaac gacgtcgatc
     1681 gaattgtcgt cggcctaccg ttacacacga gcgggcgcga gggtgagctg gcggcgcggg
     1741 cgcgcgcgtt tggcgagtgg ctgggcggtg tcagcggacg tccggtcatc tatttcgacg
     1801 aacgttacac cacggttgaa gccgagaatc ggctgatcga cctcggtatg acgcgacaga
     1861 aacgcaaggc gcttcgcgac aagcttgctg ctcagatcat gctccaaagc tatcttgaag
     1921 ccggctgccc cgagacagag gccgccggtg tgccgctcgc cgatcccgac gaggtggagt
     1981 tgtgaccact ctgatcatcg gctgcggcta cttggggcag cgattgggga caattctcta
     2041 cgagcagggc gagcgtgtct tcggcacggt tcgatcggcg agtcgcgcct cccttctcgc
     2101 cggcctgggc atcgagcccg ttatcgcgaa cgttctcgag cctgactcgc tgagggaatt
     2161 accggaggcc aatggggtgt tttactgcgt cgggttcgat cgcgccgccg ggtcttccat
     2221 gcggacggtc tacgtcgatg gactgcagaa cgtgatcgat aggctgcctc gatcagtgat
     2281 gcgcttcgtc tacgccagct cgaccggcgt ttacggccag accgacggtg gatgggtcga
     2341 cgaatcatcg ccgaccagtc cccagcacga atcgggcaaa gtgtgcctgg aggccgagga
     2401 tcgcgtgcgc gcctgggcgg actcaggcaa ccgctctgcg tcagccgttg ttttgcgatt
     2461 cgccgggctc tacgggcccg gacggatggt gcgccagtcg atcctggaac gaggcgagcc
     2521 gataccgggt gacccgtata aattcctcaa tttgatccac attgacgacg cggcccgggc
     2581 tgccgccgcc gcactgacga ccacccaacc tcatcgttta tatctggtct gcgacgaccg
     2641 gccggtcatg cgcaaagagt attacgccca gatggcaacg cttctgggca cgccagagcc
     2701 gcgcttcgag gtccccgggc cggacagtcc cgaagccgcc cgcgacgcga ccaataaacg
     2761 aattaacaat caccgcctca aaagcagcct gcacctcacc cttattcatc ccgatatcac
     2821 gaccggcctt ttcgcatctt tgcacacgag cacgcattcg taatcgcgag tgattgtcgc
     2881 gtcagtccga gcccccgtcg ttttcgggcg gaggtcgact agggcggggc ccgtcggaca
     2941 agacttgaca ttcattccgc gatggccgcc tactacatgt tgcagacgat taggcagctc
     3001 aagttcgtga tggctggacg atcagaatcg gtttctgctg tcggaatcag agttcggagc
     3061 cgccgttgat accgaaacca ttgcgagcca gcctcgcggg cttcttatgc gtggtggtgg
     3121 cagggttggg atcgctcgtg cttgctgacg cgaaggcgag cgcgcgcatc gaggaagcgg
     3181 tcaggtggat ctcgaggtgc atcccttcct tttcaacgag cgccacggct tcgcggatca
     3241 agccaaacag gcctccgatc gactcgattc cgatgttcag ctccgctggc ttcgaaaccg
     3301 gcggcttcgc agcggcaagc cggttcactg ggccgatcgc cgatcgcggg tcgctcgcgc
     3361 aggtcaaaga ggcagcccaa gggagagtgc gacgcgggct cgatgaattg aactcagcgc
     3421 ttcgatcgat cccgccagac tcgcccgacc gcccgttcat gaccttccag atccacgcga
     3481 ccatgggcct cctgagcatg tatgacggga aattcggcga ggccgccggt tggtttgaca
     3541 cggccctggc ggagagcgcg gacaacgacg ttccccccgg tctgcgggcc aactttgagg
     3601 cgatgctggg ggttattcat ctgcggcgtg gcgaaacgga aaactgcctc gaatgtcgcg
     3661 gcccgtcgag ctgtatcttc ccaatcgact ccgaggccgt gcatcagttt ccatcaggct
     3721 cgcgcgaggc gatccgccat ttccaaaggt acctcgacaa gcggcccgac gatctggggg
     3781 tacgctggct cctcaatatt gcctacatga cgctgggaga atatcccgac aaagttccac
     3841 agaatcaatt gatcggaatc gagcctctgc attcccgact cgacgtcggc cggtt
//
LOCUS       DATAQL010000192         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5211597, whole genome shotgun sequence.
ACCESSION   DATAQL010000192 DATAQL010000000
VERSION     DATAQL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5211597"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..795)
                     /locus_tag="VHS97_03215"
     CDS             complement(178..795)
                     /locus_tag="VHS97_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447233.1"
                     /translation="MNDATTNDPETWVDQHEDSLFRFALVRVRDPDTACDLVQETFLA
                     ALRVRETYSGRSSVRTWLVAILKHKISGHLQRIGREQRFQGGSVCEDRAECTFDRRSR
                     WRTAPLDWGSDPLSEYERREFWDVLGRCLSRLPADLADAFLERELDGLSREVICEDMK
                     ITPENLSVKLFRARLLLRGCPELHWFRVHSAISKGTQASAWAQAR"
     gene            1299..1622
                     /gene="trxA"
                     /locus_tag="VHS97_03220"
     CDS             1299..1622
                     /gene="trxA"
                     /locus_tag="VHS97_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX3447234.1"
                     /translation="MSAINLTDRTFAAEVLQSEVPVLVDFWAPWCGPCRMMSRIVDEV
                     SAELGDRARVVKVNIDEALATAVRYGITSIPSFAVIRGGEVQQRFSGVVPKDRLLRAL
                     DPNLR"
     gene            1722..2162
                     /locus_tag="VHS97_03225"
     CDS             1722..2162
                     /locus_tag="VHS97_03225"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HEX3447235.1"
                     /translation="MTSDSRPTDRRNRCPGIWTYAEVRAVLPYVASIMGSLREHRLDC
                     LRHRLSVQRFAAKSGRPTRDTLLDRAEAAREEGRAVAEYEKTLAELESLGIRCLDSAA
                     GLAIFTVYESGYLSEYVYDLFDSQPVRFPDEHVPGGKGKGRRGK"
     gene            2432..3043
                     /locus_tag="VHS97_03230"
     CDS             2432..3043
                     /locus_tag="VHS97_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447236.1"
                     /translation="MDRANGVWIEELRQPGPRREAALADLRADVLRGLRSALAGRPGM
                     DDAFFEDTVQETLLKVLDRLETFAGRGRFVSWALAIAVRVAQTTIRKQRWQGVPLDRF
                     KDENGGPAVDVADQSLGPERASLRSELLVAMHRVIREQLTDKQWLALTAELNGMPQDE
                     IARQLGSNRNAVYKLTHDARRKLRAGLESAGFGADSLLEAWKS"
     gene            3060..>3258
                     /locus_tag="VHS97_03235"
     CDS             3060..>3258
                     /locus_tag="VHS97_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447237.1"
                     /translation="MNLSVDQIGALLQAVSRTRDRELTCDECLGELGSFAELTLEGRP
                     VEEAYDLVQQHLQICDECREDF"
BASE COUNT          620 a         1016 c         1041 g          581 t
ORIGIN      
        1 agacggtggg cactggccct caggcattcc cataccggcc gctcccggtc gagtgattcg
       61 gattcagagt gcgagacctt ttcgcacgat gcccacagcc acgacaaggc accgagggcc
      121 tcctcgcgga gaccgcgtct ggtccggtcg ggcatgggtt acctcctttc aggggtgtca
      181 cctggcctgg gcccaagccg aggcctgagt acctttggat atcgctgagt ggacacggaa
      241 ccagtgcagt tccgggcagc ctcggaggag caggcgggcc cgaaaaagct tgaccgagag
      301 gttctccggg gtgatcttca tatcctcgca gataacttcg cggctgaggc cgtcgagctc
      361 gcgttcgagg aacgcgtcgg ccaggtcagc ggggagcctt gacaggcatc gcccaagcac
      421 gtcccagaat tcacgacgtt catactcgct gagcgggtcg ctgccccaat cgagcggagc
      481 ggtccgccaa cggctgcgac ggtcgaacgt gcattcggcc ctgtcctcgc acacactgcc
      541 cccctggaag cgctgctcgc ggccgatcct ctggagatgg ccggagatct tgtgcttgag
      601 gatggcgaca agccaggtgc ggactgacga gcgcccggag taggtctcgc ggacccggag
      661 cgccgcgaga aatgtctcct gaaccaagtc gcaggccgta tccgggtcgc ggacccgcac
      721 gagggcaaac cggaagagac tgtcctcgtg ctggtcgacc caggtttcgg ggtcgttggt
      781 cgttgcatcg ttcattgttt ggatctatcc tcgaaaacat cattatttta acaactactt
      841 cttctatact agacgccctt tcttcgcttg ctattcggtc cggcccttgt gttcttccgg
      901 ttatcggccc ctatctgtta gtcgaggggc ggcgacttgc attacaaaaa gaatccttga
      961 gccgcggaat cccgcgcgcg aggggtgacc gcggtcaccg aggaaacgtc ttccggctgt
     1021 tgcgggcgca ggcctggcgt ctcttcgccc gctcgggctg atttgacgtc agaccgcctg
     1081 aaagcctcat cccagtcgcc tgacggccac tgatcagtcg aaccgaacag ccaccgacag
     1141 cgcctatccg taagacgtcg cgggcgcagc ggtccttacc tcaaacgaca gaaaaaacgc
     1201 accacgcgga atcttgcctg tgcgaggtaa gagatcggga ggcagccccg tcctcttcct
     1261 gtgatcgatc cgcaactttg agcgagagag aaatgaccat gtctgcaata aatttgaccg
     1321 atcgcacgtt tgccgcggaa gttcttcagt cggaggttcc ggtactcgtc gacttctggg
     1381 ccccgtggtg cggcccgtgc cgcatgatgt cccggatagt cgatgaggtg tcagcggagc
     1441 ttggagaccg cgcccgcgtc gtcaaggtga acattgacga ggcgctggcc acggccgttc
     1501 ggtacggcat cacgtcgatc cccagcttcg ccgtgattcg cggcggtgaa gtccagcaac
     1561 ggttctcggg ggtggtaccg aaggatcgcc tcctaagggc gctcgatccg aaccttcgct
     1621 gactgaggtg cgcgccagta ttctcgaacg gcgccggccg gactttggac ctggaggcag
     1681 gtgactgaag catccgaccg gcatcgcgcc aggaggacta aatgacaagc gacagccgac
     1741 cgaccgatcg tcgcaaccgt tgcccgggca tctggaccta cgccgaggtt cgggccgtac
     1801 ttccctacgt cgcctcaatc atgggctcgc tccgcgagca caggttagac tgcctgcgcc
     1861 accggctttc cgtccaacga tttgccgcca agtccggcag gcccacgcgg gacacactgc
     1921 tcgaccgggc tgaggctgcc cgcgaggaag gccgagccgt tgcggagtac gagaaaaccc
     1981 tcgccgagct tgagtcgctc ggcatccgct gcctcgactc cgccgccggc ctggccatct
     2041 tcacggttta cgagagcggg tacctcagcg agtacgtcta cgacctgttc gactcgcagc
     2101 ccgtacgctt tccggacgag cacgtgcccg gcggcaaggg caagggacgt cgaggaaaat
     2161 agcttgggta tgtcaagcta ggacgcaaca gaccaacgcc aaacgtggtg ggaggccgca
     2221 actcgatatc ggtcttgaga tgagttacgg agaggtggcc ccggtgcggc aggcaggcta
     2281 cactaagagc ggcaacgccg agagggtcgc tggcgtcgcc ttggatccgt agtccaactt
     2341 ccacgtggtc ggtacgttgc tcgacttccc ggcggcactg ccggacgccc gcagttgaca
     2401 ggcggcccgg cgttgcccgc ggacactcga aatggatcgc gccaacggcg tctggattga
     2461 ggaactaagg caacccggcc cccgccgcga ggccgcgctg gcggacctac gcgccgacgt
     2521 gctccgggga ttacgcagcg cgctggcggg acgcccgggc atggacgatg cgttctttga
     2581 ggacacggtg caagagacgc tcctgaaagt gctggaccgc ctggaaacat tcgccggtcg
     2641 ggggcggttc gtgagttggg ccctggccat cgccgtccgc gtggcgcaaa ccacgatacg
     2701 gaaacagcgg tggcagggcg tgccactcga ccggttcaag gacgaaaacg gcggcccggc
     2761 agtggacgtg gccgaccaat cgctggggcc cgagcgtgcg tcgctccgct cggagttgct
     2821 ggtcgcaatg catcgggtca tccgcgagca gctcaccgac aagcaatggc tcgcactgac
     2881 cgccgaactg aacggaatgc cccaggatga gatcgcccgg caactcggca gcaaccgcaa
     2941 cgccgtctac aaactcacac acgacgcccg ccgcaaactc agggcagggc tcgaatccgc
     3001 gggatttggc gctgatagct tgcttgaggc gtggaagagt taatagcgag gcttatggta
     3061 tgaacttatc agtcgaccag atcggcgccc ttctgcaagc cgtcagccgc acgcgggatc
     3121 gagagctgac ctgcgacgag tgcctggggg aactgggcag cttcgccgag cttaccctcg
     3181 aggggcggcc agtcgaagaa gcctatgacc tcgtccagca acacctgcag atttgtgacg
     3241 agtgccgcga ggactttg
//
LOCUS       DATAQL010000193         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3533004, whole genome shotgun sequence.
ACCESSION   DATAQL010000193 DATAQL010000000
VERSION     DATAQL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3533004"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VHS97_03240"
     CDS             <1..1615
                     /locus_tag="VHS97_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX3447238.1"
                     /translation="RFVFDPGRTPRCLIPETISGGVGVIDFDGDGWLDVYCVQGGTLT
                     APDLPTGGPVSLIPDPKPGDCLFRNQGDGTFREVTRQARIDRLAWGRGYGMGVTVGDY
                     DNDGRPDLFITRLRRYDLFRNRGDGTFEDVTERAGLAGVQYNPTSAAFADLDGDGDLD
                     LYVCHYVRVDLDHPPLCKNRRGEYCYCDPAKFEPAADHVFRNEGGRFVDVTTEAGFTD
                     RDGPGLGVVAADLDDDNRIDLYVANDGTANFLFRNRGGFRFEEIGVTAGVAGSAEGGF
                     QAGMGVASADLDGDGRLDLLVTNLYLEGTTLYHNLGQGMFADHSASSGILPATRYLLG
                     FGIAVFDASNEGRLDVAITNGNVNDFRPFYPSAMPSRLYKGRSGGRLVDVSEHAGPPW
                     AVPRLGRGLAAGDLDNDGRIDLIVLAQDGPLAYFHNQTERALHFVTFRLEGTDSNRDG
                     VGARVVVATASGRQVAQRVGGGSYLSACDGRLHFGLGASTTVESVEVRWPSGRLDRWK
                     NIAADTGYLLREGDPAPRPLAGMRKRMKN"
     gene            complement(1701..2915)
                     /locus_tag="VHS97_03245"
     CDS             complement(1701..2915)
                     /locus_tag="VHS97_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HEX3447239.1"
                     /translation="MRTHRRRRDRDRPHVALFVETSINYGRGVLRGIARYLRECGPWS
                     IFLEQHEIGAKLPDWIDRWDGDGIITRYDHPQILETELPTVVLFDRTDEWLGLPRILN
                     DNLAVGRMAAKHLLERGFESFGYYGIPSEYWSDLRWRGVQEMVKVVEGVPCHCRHDPG
                     DRTQDWESQQNALAEWISSLPRPLGLIACNDIHGLRALDACRRAQLAVPEEVAVVGAD
                     NDVELCDLSDPPLSSIDFNRERVGYEAAALLDYLMMGETPSSLAQLIPPLGVITRQST
                     DILAIADRYVAQALHIIRRRACSGIDVQSVLERLPLCRRSLEQRFRQCLGRSPGAEIQ
                     RVRIEQAKMLLAETDLTVEVVSRRSGFSKPAYFSTAFRNQTGIAPSMYRRLADPRGSP
                     FADVSAEVVTPL"
     gene            3351..4388
                     /locus_tag="VHS97_03250"
     CDS             3351..4388
                     /locus_tag="VHS97_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3447240.1"
                     /translation="MRLRRRGFTLIELLVVIAIIAVLIALLLPAVQSAREAARRIQCT
                     NNLKQIGLAMHNYHDVMGTFPPGSIADPGWGGTWWNWPAFILPQIEQTSLYNAINFSH
                     SNIARSATANVPAPNDTNSTVWLTVVNGFLCPTDPAVSNGVLPNMSWVCTENYGQMVT
                     GAVTCYVANWGDMKTGNLTFDFYSSESTSGTGPNWGCGGIFSGMFGDCSNGKAVGLRD
                     ITDGSSNTFLAGECSPNMNGSLMWVNGDGTYASTVIPLNWKTDLKDGVVDPTDGTTCG
                     LAYLNNFTQALHCWRNQTVNYAFKSYHPGGVNFAMVDGSVKFLKQSINAPTYRALGTR
                     GYGEVISADAI"
BASE COUNT          833 a         1359 c         1338 g          861 t
ORIGIN      
        1 ccggttcgtt ttcgaccccg gtcgcactcc gagatgcctg attcccgaaa ccatctcggg
       61 cggcgtgggc gtgatcgatt tcgacgggga cggctggctc gatgtttact gcgtgcaggg
      121 ggggacgcta acagcccccg acctccccac gggtgggccg gtgtcgctga ttccggatcc
      181 gaagccaggc gactgcctat ttcgcaacca gggggatggg acatttcgcg aggtcacccg
      241 gcaggccagg atcgaccgcc ttgcgtgggg gcggggttac ggcatgggtg tcaccgtggg
      301 cgactacgac aacgacgggc gtcctgacct gttcatcacg cgtctgcgcc gctacgacct
      361 gttccgcaat cggggcgacg ggaccttcga ggatgtgacc gagcgcgcgg gtctggccgg
      421 ggtgcagtac aatcccacct ccgcggcctt cgccgatctc gacggggacg gcgacctcga
      481 cctttacgtc tgtcattatg tccgcgtgga cctggaccat cccccgctct gcaagaacag
      541 gcgaggggaa tattgctact gtgatccggc caagttcgag ccggccgccg accacgtctt
      601 tcgcaatgaa ggcgggcgtt tcgtcgatgt gaccaccgag gctggattca ccgaccggga
      661 cggaccgggc ctcggagtgg tggcagcgga ccttgacgac gacaatcgca tcgacctgta
      721 cgtcgccaac gacggaacgg ccaacttcct attccgcaac cggggcggat ttcgatttga
      781 ggagatcggg gtgacggccg gcgtcgccgg cagcgccgag ggtgggttcc aggccggtat
      841 gggtgtcgcc agcgctgacc tcgatggcga tggccggctt gatctcctgg tgaccaatct
      901 ttaccttgag gggaccacgc tgtaccacaa cctgggacag ggcatgttcg ccgatcacag
      961 cgcctcgtcg ggaatcctcc ctgccacccg gtacctgctc ggtttcggga tcgcggtatt
     1021 cgacgcgtcc aacgagggtc ggctcgacgt ggcgatcacc aacggcaatg tgaatgactt
     1081 tcgtccgttc tatccctccg ccatgccgag ccggctctac aagggacgtt ccggcggacg
     1141 tctggtcgac gtttcggagc acgcgggacc gccctgggcc gtccctcggc ttggccgtgg
     1201 cctggccgcc ggcgatctcg acaacgacgg ccgcatcgac ctcatcgtcc tggcccagga
     1261 cgggccgttg gcctatttcc ataaccagac cgagcgcgcc ctccacttcg tgacgtttcg
     1321 gctcgagggg acggactcca accgggatgg cgtcggcgcg cgagtcgtgg ttgccaccgc
     1381 ctctggccgg caggtcgcgc aacgggtggg gggcgggagc tatctttcgg cctgtgacgg
     1441 tcgactccac tttggtctgg gagcgtcaac aacggttgag tccgtcgaag tccgctggcc
     1501 ctccggtcgt ctcgatcgct ggaagaacat cgcggcggac accggctacc tgctccgcga
     1561 gggtgatcct gcgccccggc ctctggccgg gatgagaaaa aggatgaaaa attaaggatg
     1621 agttggaaat cgctaaatca cgaaacagat gtttgtttca actttccgcc ttcatccttc
     1681 atgattccgt tcgccggtgt tcatagggga gtaacgacct cggcgctcac gtcggcgaaa
     1741 ggtgatccac gcgggtcggc caggcggcga tacatcgacg gtgcgatgcc ggtttggttt
     1801 ctgaaggcgg tgctgaaata ggcgggcttg ctgaatcccg atctacggct gacgacttcg
     1861 acggtcaaat ccgtctcggc caggagcatc ttggcttgct cgatccggac tcgctggatc
     1921 tcggctcctg gcgagcggcc caaacactgt cggaaccgct gctccagtga cctccgacag
     1981 aggggcaacc gctcgagcac cgactgaacg tcgatgccgg aacaggcacg acggcggatg
     2041 atgtggagag cctgtgcgac ataccggtcg gcgatcgcga ggatgtcggt cgattggcgc
     2101 gtgattaccc cgaggggtgg gatgagctgt gccaacgagg aaggagtttc acccatcatc
     2161 aggtagtcga gcaatgccgc ggcctcataa ccgactcgtt cccgattgaa atcgatgctg
     2221 gagagaggag ggtccgagag gtcgcaaagt tccacatcgt tgtcggcccc aacgacggcg
     2281 acttcctccg gcaccgccag ctgggcgcgc cggcacgcgt cgagggctcg caagccgtgg
     2341 atgtcattgc aggcgatgag gccgagtggc cggggtaaac tgctgatcca ctcggccagg
     2401 gcgttctgct gcgactccca atcctgcgtc cggtcgcccg gatcgtgtcg gcagtggcac
     2461 ggcacgccct ccacgacctt gaccatctcc tggacgccac gccagcgtaa atccgaccag
     2521 tactcactgg gtattccata gtacccaaac gattcgaatc cccgctcaag caagtgcttg
     2581 gccgccatcc ggccgacagc aaggttatcg ttgaggattc gcggcaggcc gagccactcg
     2641 tcggtgcgat cgaacaagac cacggtgggg agctcggtct ccaagatctg cggatggtcg
     2701 tagcgagtga tgatgccgtc gccatcccag cggtcgatcc aatcgggaag cttggcgccg
     2761 atttcatgct gctcgaggaa gatggaccac gggccgcatt cgcggagata ccgcgcgata
     2821 ccccgcagca cccctcggcc gtagttgata gaggtctcga caaacaacgc cacatgtggc
     2881 cggtctcgat cccgtctcct tcgatgggtg cgcatgggac cagctcccct cgcatggaat
     2941 gtgcggatcc gaccttaacc cctccccgac cgcgctcgac cgccgccagc ccgatgccga
     3001 attataaccc tcgctcacaa ctaagctcga tcgaaattga tgcgcaaaat cgaaattctt
     3061 tgcgcaagtt gggatagacc gaccgcgcgc ggggcattct atttacttga acgagaggcg
     3121 tcacgcgctg caatcctcct tacttgttta gtcagttaac attttcctag cagtcgtcaa
     3181 tcggggcgca ccccagtgac gaccggaggg atgcaacaga acttcgatca ccatcgcctc
     3241 ttgtgtggtt cagtttcagc ggtccacttc agtcagccgt tttcatgaca ggacatatca
     3301 tatcacactc tcatctaatt gatccccaca attcgcggag gaggcgaact atgcgtttgc
     3361 gacgtcgcgg tttcacgttg atcgagctac tggtggtgat tgccatcatt gcggtcttga
     3421 tcgctttgtt attgcccgcg gtacagtcag cccgtgaggc ggccaggcga atccagtgca
     3481 cgaacaatct caagcagatc ggcttggcga tgcacaacta tcacgacgtc atgggaacct
     3541 ttccgcccgg atccatcgcc gaccctggct ggggaggcac ttggtggaac tggcctgcgt
     3601 tcattcttcc tcagatcgag cagacgtcgc tttacaatgc gatcaacttc agccacagca
     3661 atatcgcgag aagcgccacc gcaaacgtac cggctccgaa cgacaccaat tcgacggtct
     3721 ggttgacggt agtcaatggc ttcctctgcc ccacggatcc ggcagtctca aacggcgtgc
     3781 tgcccaatat gagttgggtt tgtaccgaga attatggaca gatggttacc ggggcggtga
     3841 cctgctacgt cgccaactgg ggcgatatga agaccggcaa tttgaccttc gacttctatt
     3901 cctcggagag cacctccgga actggcccga actggggctg cggtggcatt ttcagtggaa
     3961 tgttcggcga ttgcagcaac ggcaaggcag ttgggctccg cgatatcacc gacggcagct
     4021 ccaacacatt cttggccggc gagtgttcgc caaacatgaa cggctcgctc atgtgggtca
     4081 acggcgacgg cacctacgcc tcgaccgtca ttccgttgaa ctggaagacc gacctcaagg
     4141 acggcgtggt ggacccgacc gatgggacca cttgcggcct cgcctatttg aacaatttca
     4201 cacaggccct gcactgctgg cgcaaccaga cggtcaacta cgccttcaag agctaccacc
     4261 ccggcggcgt caacttcgcc atggtcgacg gctcggtcaa gttcctcaag cagtcgatca
     4321 acgcgccgac ctatcgcgcc ctcggcaccc gcggctacgg cgaggtcatc agcgccgacg
     4381 cgatctgacc c
//
LOCUS       DATAQL010000194         4133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_937368, whole genome shotgun sequence.
ACCESSION   DATAQL010000194 DATAQL010000000
VERSION     DATAQL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937368"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /locus_tag="VHS97_03255"
     CDS             complement(<1..2159)
                     /locus_tag="VHS97_03255"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447241.1"
                     /translation="MAQAAATAPGMVNELFDRESTRVRTDEQLLQQFISRSDESAEAA
                     FALLVERYGPIVHRVCFSILGNSHDTQDAAQAVFLVLARKARSIRKPESLGPWLHGVS
                     VRVARRAKSASGRRRVAEQRKAEMMLELDSGESGPEPMDFSELHEEINRLPEKYRLPI
                     IICYMQGKTQTQAARMLGWPLGTVQIRLHRGRERLRSRLTRRGAGLIALTGSDLTTSL
                     SSTPGAFGPEWTETTARAAVRFAAGKGTAGLVAPPVSGLAETVLAAMLGESLKLIHLT
                     AIALFLVLGGICLTGLRADKTRLLVSRLELTPAPAPLAKPEPATRAANSTPLFHGADN
                     NDPATESILDIVEQPRTAQPPEGSFENSTTLAPTPSDHPDSDVALPASPQSPDRFRSL
                     GVAGKRSEKTLSTGRELFERLWVKNDQRGHGGDGLGPVFNGQSCVACHNLGGSGGAGT
                     VGSNIEILTATGTPGGGAGYSYSFSMDFGAGRFEYRIGGDSQVQSGRPQAAHAPLLTG
                     IHPGFRESNSIVLHRYGTDPAYHGWRGSVLGSHGSVSIQSSERNPPPLFGAGQIDAIP
                     DGAIEAAARRKFPGSAQVNGRVSRLKDGRVGRFGWKAQTATLAEFVRSAAAGEIGLEI
                     PGRHQAADPRMPGLAATGLDMDESECDALVEYVRSLAVPVAIKPADEKRSAQVKAGES
                     TFKSIGCTVCHMPKLGEVDGLYSDLLLHDMGPQLADA"
     gene            2515..3981
                     /locus_tag="VHS97_03260"
     CDS             2515..3981
                     /locus_tag="VHS97_03260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX3447242.1"
                     /translation="MTQASPADVGLTAKLREHFGFRRFRPGQEAAVRAVLAGRNTLVL
                     MPTGSGKSLCFQLPSLEMEGATIVVSPLISLMKDQTDSLQQKGHDVVAINSSLTPRQR
                     HEAEVSIAKGQMQFVYTTPEQLVNHDFRAVLKQTSIDLFVVDEAHCVSQWGHDFRPDY
                     LELGSVIEDLGHPTVLALTATATEEVVDDILRQLRIPDAEIVHTGFYRPNLYLAVAVT
                     PDEDQRRERLLEFLGQEEGSGIIYASTVKAVSELAEFLATKGFSVAPYHGRLKASVRA
                     ETQDRFLKGEVPLLVATNAFGLGIDKPDIRFVVHANLPGSIEAFYQEFGRAGRDGQPG
                     RCTLLYSPEDRKVHTFFQSRRYPTAEDLVNAHHTVRRVADQLPTFADLEAISPLPKTR
                     LKQAISLLRTRGVVKEDLSGHYHLVEPNLGHDDLARFVREYEDRDERDRLKLQRFVEY
                     AETRACRWNSLLDYFGGNDPVSDPCGHCDVCEPVAAAP"
BASE COUNT          790 a         1294 c         1220 g          829 t
ORIGIN      
        1 tcagcgtcgg cgagctgggg gcccatatcg tgcaaaagca ggtcgctata gagcccatcg
       61 acttcaccca acttcggcat gtggcaaacg gtgcacccga tcgacttgaa ggttgattcg
      121 ccagccttca cctgtgcgga gcgcttttca tcggccggtt tgatggcgac aggaaccgcc
      181 aggctgcgca cgtactcgac cagggcatcg cattcacttt cgtccatgtc cagtccggtc
      241 gcggcgagcc ccggcatgcg cgggtcagcg gcttggtgac ggccgggaat ctcgagcccg
      301 atctcgccgg cggcggcgga gcggacaaac tctgcgagag ttgccgtttg ggccttccac
      361 ccgaaccggc cgacgcgccc gtctttcagg cggctcactc gtccattgac ctgtgccgac
      421 ccgggaaact ttcgcctcgc ggcggcctcg atcgccccgt cggggattgc gtcgatttgt
      481 cccgctccga acaagggcgg tgggtttcgc tcggagctct ggatcgagac cgacccatgc
      541 gaaccgagca ccgacccgcg ccatccatga tacgcgggat cggtcccata acgatgcaac
      601 acgatgctgt ttgattctcg aaagccggga tgaattccgg tcaggagtgg cgcatgggct
      661 gcttgtggac gacctgactg cacttgggaa tcgcctccta tccggtattc aaaccgcccg
      721 gcgccgaagt ccatgctgaa cgagtaggag taacccgcgc ctccgccagg cgtgccagtc
      781 gccgtcaaga tctcgatatt cgagccaacc gtgccggcgc ctcccgatcc tcccagattg
      841 tggcatgcga cgcacgattg tccgttgaaa accggaccga gcccatcacc cccatggcct
      901 ctctggtcat ttttcaccca caatctttcg aacagttcgc gtcccgtact caatgttttc
      961 tctgaccgct tacctgccac gccgagactc cggaaccggt ctggtgattg cggcgaagcc
     1021 gggagcgcga catcgctgtc agggtgatca ctcggtgtcg gcgccaaagt ggttgaattc
     1081 tcaaagcttc cttcgggcgg ttgggcggtc cgcggctgct ctacaatgtc taggatcgac
     1141 tctgttgccg gatcgttgtt gtcggctccg tggaagagag gcgtagagtt cgctgctctc
     1201 gttgccggct ccggtttcgc caagggagcc ggcgccggcg tgagttcaag gcgtgaaacg
     1261 agaaggcgag ttttgtccgc cctaagtccg gtcaaacaga tcccgcccaa gacgagaaag
     1321 agtgcgatcg cggtcaggtg aatgagcttc aaggattcgc caagcatcgc cgcgagcacc
     1381 gtctcggcca gtccggacac aggcggcgcg accagcccgg ccgtcccttt gccggcagca
     1441 aaccggacgg cggcgcgcgc ggtcgtctcg gtccactccg ggccaaacgc accgggtgtc
     1501 gacgagagcg atgttgtcag gtctgagcct gtcagagcga tcaggccggc accccggcgt
     1561 gtcagccttg accgcaatcg ctccctccct cggtgcaagc gaatctgaac tgtcccgagc
     1621 ggccagccga gcatccgcgc ggcctgcgtc tgtgtcttcc cttgcatgta gcagataatg
     1681 atcggcaacc gatatttttc aggaagccgg ttgatctcct catggagctc ggaaaagtcc
     1741 ataggttccg gccccgattc cccgctgtcg agttcaagca tcatttccgc cttcctctgt
     1801 tcggcgacgc ggcgccgccc tgacgcactc ttcgcgcgtc gcgccacgcg gacggaaacg
     1861 ccgtgtaacc agggacccaa cgactcgggc tttcgaatgg agcgggcctt cctcgccagc
     1921 acgagaaaca ctgcctgagc cgcgtcctgc gtgtcgtgtg aattgccaag tatactgaag
     1981 caaactcggt gaacgatcgg gccataccgt tcgacgagaa gggcaaaagc ggcctcggcg
     2041 gactcgtccg atcgggagat gaattgttgc aagagttgct cgtcagtccg gactctggtc
     2101 gactcacgat cgaagagttc gttgaccatg ccaggtgctg tcgccgccgc ttgtgccatg
     2161 cgtcgtcatc ctttcgcttt tggaccgcct ttcacgttag tgcctcccaa cgcggcttac
     2221 catttcagaa tctcgggatt tttattgcgg gcttgtcctt tgagccgacg acgaaccaga
     2281 gcgcccaatg acgtgcttca catcatgcgt ccctctgtcc agcgccagtc cttgccaatc
     2341 ttcggccaga aacggactca tgcgggtcat ccgcaggacg gccgcgaagc gcggagtgat
     2401 tccggacgac ggcccgcgat ttgcacgttg tctgtgcgtc gcgaatgaac tcagggactg
     2461 agcctgtccg ccttgaccgc gaggcttggt cgggcggcga agataggacc tatcatgact
     2521 caagcgagtc ctgccgatgt cgggctgacg gccaagctgc gtgaacactt tgggtttcga
     2581 cggtttcgcc ccggccaaga agccgcggtc cgcgcggttc tggccggccg aaacacgctt
     2641 gtgctcatgc ccacggggtc gggaaaaagc ttgtgctttc aactgcccag cctggagatg
     2701 gaaggcgcca ccattgtcgt cagccctttg atctcgttga tgaaggatca gaccgattcc
     2761 cttcaacaaa aagggcacga tgttgttgcg atcaacagct ccctgacccc gcgacagcga
     2821 cacgaggccg aggtgtcgat cgcgaaggga cagatgcaat tcgtttacac gacgcccgag
     2881 caactggtta accacgactt tcgggccgtg ctcaagcaaa cgtcgatcga tctgtttgtt
     2941 gtcgatgaag cgcactgcgt tagccagtgg ggacacgatt tccggcctga ctatctcgag
     3001 ctcgggtcgg tgatcgaaga cctgggacat cccacagtgc tggccttgac ggccaccgcc
     3061 acggaagagg tcgtggacga cattctgcgc cagctacgaa ttcctgacgc cgagatcgtc
     3121 cacacgggct tttatcgacc caacctttat ttggccgtgg cagtgacccc ggatgaggat
     3181 caaaggcgag agcgacttct cgaattccta ggtcaagaag aagggagcgg gattatctat
     3241 gcctcgacag tcaaagcggt aagcgaactt gccgaattcc tggcgacaaa aggtttctcg
     3301 gttgctccct accatggccg attgaaggcg tcggttcgag cggagacgca ggaccgcttc
     3361 ttgaaaggag aggttccctt gttggtcgcg accaacgcct tcggcctggg aatcgacaag
     3421 ccggatattc gtttcgtggt ccacgccaat ctgccgggct cgatcgaggc gttctaccag
     3481 gaattcggcc gggccggccg cgatgggcag cccggccgtt gcacattgct ctattcgccc
     3541 gaagaccgga aggtccacac attctttcaa agtcgtcgtt atcccaccgc cgaagatctg
     3601 gtgaacgctc accacaccgt ccggcgcgtc gccgatcagc tgcccacttt cgcagatctc
     3661 gaggcaatct cacctttacc caagacgcgt ctcaaacagg cgatctcact gttgcgaacc
     3721 cggggggtcg tcaaagaaga tctatcgggg cattatcacc tggtcgagcc caacctgggt
     3781 cacgacgatc tggcacgatt cgtgcgcgaa tacgaagatc gtgacgaacg cgaccggctc
     3841 aaactgcagc gattcgtcga atacgccgaa acgcgagcct gccggtggaa ctccttgctc
     3901 gattatttcg gcggcaacga cccggtctcc gatccatgtg gtcactgcga cgtctgcgag
     3961 cccgtcgccg ccgcaccgtg accgagtcct cgcccccacg aaccagcgta ttgtgcgtaa
     4021 gatcctcgag gatgcgggac ctacgtcgcg attgaccctg accgagaccg aactgaatca
     4081 gccgttcgcg cgccgagtgc tggcgacaaa gcgcccctcg ccctgtcggc gcg
//
LOCUS       DATAQL010000195         6616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3228218, whole genome shotgun sequence.
ACCESSION   DATAQL010000195 DATAQL010000000
VERSION     DATAQL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3228218"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..2505)
                     /locus_tag="VHS97_03265"
     CDS             complement(118..2505)
                     /locus_tag="VHS97_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447243.1"
                     /translation="MVRKPAVADALLLMLAGLIHGAGTAASAAPPKINNLSPLGVQRG
                     VATELTVSGANLAGSPRLIAPFRFRIEPASPPAKSDAANWKVRLTAAADVAVGIYPIR
                     IQTDDGISPSFLLPVGQLPQVAEKEDNSSFELAQVLPELPIVVEGQVAGNDVDYFRFH
                     GTKGELIVVDAQCARIGSGIDPTIRLTTAAANRAYISSADDSPGLQTDARLTAVLPAD
                     GDYVVELSDSRYQGGGRPVFRLVIGAVPMAAEVYPLGGRAGETVGLEFRGGTLEGVKV
                     AAAAMNPLLGTELVVPRISSAMLGVGTGAGGVPGGGQTLDLESLSALVASSYPEIREP
                     ADPEAPPARAVAPVVLNGRIDPAGDDDRFVLAVTPGQRLRIKVVAYEVGSALDAVLRV
                     LGNGGSALANADDMTTPLPPVNGQPQSLVIPDPTLEFTVPGGTNEITLVLRDLEDRGG
                     VGFPYRIVVEPLLPDFQIVLTDSEVSVPKGGVVAVGVTVQRRGYTGPINTTIADPPTG
                     LSVRPGTIAAGQTTGILSLSAAADASFPAVPIKLVGRAQGVNGPIERLAYKPIVFAQQ
                     AKLATCSITEYGLTAAPALAEPVVFDTPPALVEVAHGLSATVPVKVTRSQGADAALAI
                     SPLPLPPGLTIAAATIAEKASDGQLTVKAAVTAPLGVMTVGFQAKGKFGGQDQTIDLP
                     AVTLSIVPPATLAIAAPGLEIKPGATVELAGSIVRKGGYDGAVTVKINGLPAGLKADP
                     VTVADKESRFAVKIIADAKAAVTSAETQVALTYQVEKKDYSVGPVPLAVKVVPIK"
     gene            2984..4003
                     /locus_tag="VHS97_03270"
     CDS             2984..4003
                     /locus_tag="VHS97_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3447244.1"
                     /translation="MSTRDRWFQHDIRRPKAPVVAPVDSLAPRPAPKDAVILFDGSNL
                     DAWRSPDGGPARWKVTGGAMEVAPGTGAIETKAKFGDVQLHVEWASPNPPVGTGQDRG
                     NSGIFLMGEYELQVLDSYHADTYADGQAGAIYGQYPPLANASLPPGEWQTYDIAFRRP
                     RFDNSGKLLEPARLTLIHNGILIQNNEELLGRTSWLESLPYEAHADRGPIQLQDHGHR
                     VRFRNMWIRDLPERPLPTAEDLARPQIVSLTPQVLDSFTGQYAMGSNADAPRVTITRG
                     DGHLIVKLTFRPTPLVIEPISPTEFLLPHTDGRFTFRQDQQGRVTGALFKIGDGEREL
                     TKINK"
     gene            complement(4058..4729)
                     /locus_tag="VHS97_03275"
     CDS             complement(4058..4729)
                     /locus_tag="VHS97_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3447245.1"
                     /translation="MQSPVEKAFEAVQSGDVQSLRELLTSDPKLAGARSETGLSLVLQ
                     ACYFKRPEMVELILSAAPNLDIFEVAALPSEWERGAALLAADPELAGNWSSDGFTPLH
                     LASYFGNEAMAGLLLERGADPGAVSRNGMALRALHSACSACNIGIAAMLLRRGVDVNA
                     RQHGGWTALHSAASNGDLPLVDLLLENGADPGLASDDGKTSFDVAQVRGHTAVLEALR
                     GQHVS"
     gene            4839..5282
                     /locus_tag="VHS97_03280"
     CDS             4839..5282
                     /locus_tag="VHS97_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3447246.1"
                     /translation="MADERAIADRLHSAAIHLLRRVRRVDEATGLSGAKLSALSVVVF
                     AGPISLRDLAAAEQVRPPSMTRTVRELELDGLITRETDDSDRRVTRIRATAKGEQLLQ
                     QGRGARIKLLADLLRALDVDDLSLLDEASKALERLFSAPMRDRKG"
     gene            complement(5557..>6616)
                     /locus_tag="VHS97_03285"
     CDS             complement(5557..>6616)
                     /locus_tag="VHS97_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447247.1"
                     /translation="LHCEPLETRQLLSIGQTSLGASVLASPTTAFALVSAPVAVSNST
                     PSGLPNSSVSGVATSGGLSPIFSVGTANSTNSANVPIFVTGNVNGNEVNAGDNGAGLS
                     PVTSSTITPLNPNDTSITASSTEPQPDVTVFVIPPPLAPPVLQLGPSTTPPTTVSNST
                     SLSNLDAQPPSATHFGQGDPFGGRRLYLEGINANRLSLSLIDNIDPSPAATRVNVPDV
                     SPAQPVDAPAPPPAPPVDAPESPPAPQGDQPPAPDATNVRPLPPTSEPDVEATLDLTD
                     ARVLTRSGDGDASAPNDRFASNNTSWSFSVLFGFTVVASGGYQFAIRQADRLKGRSIP
                     RWVGAERPNKRKKGLSSP"
BASE COUNT         1266 a         2070 c         2070 g         1210 t
ORIGIN      
        1 agttggccgc cagcgaagct ctttcccccc cttgtgaagg cggggccagg gatagattgc
       61 tttgtacaag tagaagtctg attattcgac ggcattgggc tttatcccag tttcattcta
      121 cttgatcggc acgaccttga ctgccagagg tacgggcccg accgaatagt ccttcttttc
      181 aacctgataa gtaagtgcaa cctgcgtctc tgcggaagtt acggcggctt tcgcatcggc
      241 gataatcttc acggcgaagc gcgactcctt gtcggccacg gtgacagggt ccgccttgag
      301 tccggcgggc aggccgttaa tcttgacggt caccgcaccg tcgtagcccc ccttgcggac
      361 gatcgatccc gccagctcga cggtcgctcc tggtttgatt tcgagcccgg gcgcggcaat
      421 ggcgagcgtg gcggggggca cgatcgaaag cgtcacggcg ggcaggtcga tggtttggtc
      481 ctggccgcca aacttgccct tggcttggaa gcccactgtc atgactccca agggcgctgt
      541 caccgcagcc ttgaccgtga gttggccatc cgaggctttc tcggcgatgg tcgccgcagc
      601 gatggtcagg cctggtggca ggggaagcgg tgaaatcgcg agcgccgcgt cggccccttg
      661 tgagcgagtc accttgaccg ggaccgtcgc gctcaagccg tgggcaacct cgactagagc
      721 agggggggtg tcgaatacaa cgggctcggc gagggcgggc gcggccgtca ggccgtattc
      781 ggtgatcgag caggtggcga gctttgcttg ctgggcaaac acgatcggct tatatgcgag
      841 ccgctcgatt gggccgttga ctccctgcgc ccggccgaca agcttgatcg ggacggcggg
      901 aaacgaggcg tcggccgcgg ccgatagtga gagtatgccg gtcgtttggc ccgcggcgat
      961 cgtcccggga cgcacgctca atcccgtcgg cggatcggcg atggtcgtat tgatcggccc
     1021 ggtgtagccc cggcgctgga cggtcactcc gaccgcgact acaccaccct tgggaacgct
     1081 gacctccgag tcggtgagca caatctggaa gtcgggcaag agcggctcaa cgacgatccg
     1141 gtacggaaaa cccacgccgc cgcggtcttc cagatcacgc aatacgagag tgatttcatt
     1201 ggtgccgccg ggcaccgtaa attcgagcgt cgggtcgggg atgaccaatg actgcggctg
     1261 gccgtttacc ggcggcaaag gagtggtcat gtcgtcggca ttcgcgagcg ccgagccgcc
     1321 gttgccgagt acgcgcaata cggcgtcgag cgccgagccg acctcatagg cgacaacctt
     1381 gattcgcaac cgctggcccg gggtcaccgc cagaacgaag cgatcgtcgt cgcccgcagg
     1441 gtcgatccgg ccgttgagca cgacaggcgc gaccgcccgg gccgggggcg cctcgggatc
     1501 agcaggctcg cggatctcgg gataactcga agccacgagc gccgacagcg attcgaggtc
     1561 aagcgtctgc cctccgccag gtactccgcc agcgcccgtg cccacgccca gcatggcact
     1621 cgagatgcgc ggcacgacca gttccgtacc caaaagcggg ttcattgcgg ccgcggcgac
     1681 cttgaccccc tccagggtgc caccgcgaaa ttccaggccg acggtctcgc cggcgcggcc
     1741 ccccaggggg tatacctccg cagccatcgg cactgccccg atgaccagcc gaaacaccgg
     1801 acggccccct ccttgatagc gcgagtcgga cagctcgacc acgtaatcgc catccgccgg
     1861 cagcacggcg gtcagccggg catccgtctg caggcccggc gaatcgtcgg ccgaggagat
     1921 ataagcgcgg ttggccgccg ccgtcgtcaa ccggatcgtg ggatcgatcc ccgagccgat
     1981 ccgcgcgcat tgcgcatcga cgacgatcaa ctcaccttta gtgccgtgga accggaagta
     2041 atcgacgtca ttccctgcca cctgcccttc gacgacgatt ggcagttccg gcagcacctg
     2101 ggccagctca aagctgctgt tgtcttcctt ctcggcgacc tggggcaatt ggccaacggg
     2161 cagcagaaac gacggcgaga tcccgtcgtc ggtttgaatt cggatcggat agatccccac
     2221 cgcgacgtcg gccgcagccg tcagcctgac tttccagttg gccgcgtcgc ttttggccgg
     2281 tggcgaggcc ggctcgatcc gaaaccgaaa tggcgcgatc aaccggggac ttcccgcgag
     2341 gttcgcgccg ctgacggtca gctcggtggc cactccacgc tgaacgccca ggggcgacag
     2401 gttattgatc tttggtggtg cagcgcttgc cgccgtgccg gctccgtgaa tcaagccggc
     2461 cagcatcaag agcagagcgt cagcgaccgc gggctttctt accatggaaa cgatctcttt
     2521 caagcaaatc gctgcaattt ccgaccagcc gttgggctcg agcacgacag gcaatcgggc
     2581 ggggcaagga gcgccatcag gcgggtcgcc acgttttgtt gaatgttaat ttaacctcgc
     2641 acgatatcat caatttgtca aggagaggtg gttgcgctcg aaagcgtgat gaccgaggtg
     2701 tggcgtatcg gcggacaccg tcggcgcggg cctttcgagc cgatcgacca acatctacaa
     2761 tagcctgatc gacgatcgcc gcacatccgc gatgagacgg gtggcgaatc aaagtcaccc
     2821 cgcttgcgcg cggtttgtag gggcgatttc aaggcgaccc atcgttttct ttttttggtg
     2881 agagcgacgc aaaatgtctg cacgatccaa ccgcctggcg tgcctctcgt tgatatcgag
     2941 tacgggacta atcgctttcc tcgtagccgg cgaggcacat aacatgtcga cccgcgaccg
     3001 ctggtttcag cacgacattc gccgtcccaa ggcgccagtc gtggcacccg tcgacagcct
     3061 ggccccgagg cctgcgccca aggatgcggt tattctcttt gatggatcaa atcttgacgc
     3121 ctggcgaagt cccgacgggg gcccggcacg ctggaaggtg acgggcggcg ctatggaagt
     3181 cgcgccggga accggagcga tcgagaccaa ggccaagttc ggggatgttc aacttcacgt
     3241 ggaatgggcg tcgcccaacc caccggtggg cacgggccag gatcgcggca acagtggcat
     3301 ttttctcatg ggcgaatacg agctacaagt ccttgattcg tatcatgccg acacctacgc
     3361 cgacggccag gccggggcca tctacggcca gtatccacca ctagccaacg cctcgctccc
     3421 ccctggagag tggcagacgt acgacattgc gtttcgccgg cctcgcttcg acaacagcgg
     3481 caagctgctc gagccggccc gactcacgct catccacaat ggcatcctca tacaaaataa
     3541 tgaagagctt ctaggcagaa catcctggct ggagtcgctc ccctacgagg ctcatgccga
     3601 tcgcgggccg atccagcttc aggaccacgg ccatcgagtc cgcttccgca atatgtggat
     3661 ccgcgacctg cccgaacggc ccctaccgac ggcagaggat ctcgctcgac cccagatcgt
     3721 ctcgcttacg ccccaggtcc tcgattcatt cacgggccag tatgccatgg gatcaaacgc
     3781 cgatgccccg cgggtcacga tcacccgcgg cgatggacac ctgatcgtaa agctcacgtt
     3841 caggcccaca ccgctcgtca tcgagcctat ctctccaacc gagtttctct taccgcacac
     3901 cgacggccgg ttcaccttcc gacaagatca acagggccgg gtgacaggcg ccttgttcaa
     3961 gatcggcgac ggcgagagag agctaacaaa gattaacaag tgaatcagaa cttgaacctt
     4021 gcatgggacg gatactgagc atcggctcgc cccggggcta gctcacatgt tggccgcgga
     4081 gcgcttccag aacagccgta tgtcctcgaa cttgggctac atcaaacgat gtctttccat
     4141 cgtcgctggc gagacctgga tcggcgccat tttctagcag gagatcgacg agcggaagat
     4201 cgccattgct cgccgcggag tgcagagcgg tccagccacc gtgctgcctc gcgttcacgt
     4261 cgaccccccg ccgcaacaac atcgcggcga ttccgatgtt gcaagccgag caggcgctat
     4321 gaagcgcccg aagcgccatc ccattgcgcg atacggcacc tggatcggcc ccccgttcga
     4381 gcaggagccc ggccatcgcc tcgttgccga aatacgacgc gaggtgcagc ggcgtgaatc
     4441 cgtccgagga ccaattgccc gctagctcgg gatcggccgc gagcaacgcg gcaccacgct
     4501 cccattcact ggggagcgcc gcgacctcga agatgtcaag gttcggggcg gcgctcaaga
     4561 tcagctccac catctccggc cgtttgaagt aacacgcttg cagcacgagt gagagcccgg
     4621 tctcgctccg cgcaccggcg agtttgggat ccgaggtcaa caactcacgg aggctctgca
     4681 cgtcaccgga ctggacagct tcgaacgctt tctcgactgg actctgcatt ggtctgcctc
     4741 caccatgtga agaagaaacg acactaaatc agccagttag ttagttagtc taactaacta
     4801 tacggttggt atgcttggcg tcaagtggag agatcgggat ggccgacgaa cgtgcgatcg
     4861 ctgaccggct gcattcggca gccatccatc tcctccgccg cgttcgacgc gtagacgagg
     4921 cgaccggcct ttccggtgcg aagctgtcgg cgctttcggt ggtggtgttc gcgggcccga
     4981 tctcactgcg ggacttggcc gcggccgagc aggtcaggcc tccgagcatg acgcgcaccg
     5041 tgcgagagct ggagctcgac ggcctgatca cgcgcgagac ggacgactcc gaccgccgcg
     5101 ttacgcggat tcgcgcgacc gcgaaaggcg agcaactctt gcagcaaggg cgcggagcca
     5161 ggatcaagct gttagccgat ttgctccggg ccctcgacgt cgacgacttg agcctgctcg
     5221 atgaggcgag caaggcgctc gaacgtcttt tctccgcgcc catgcgcgac cgaaagggtt
     5281 gacgtccggt cgttcggacg tcgatgagtg cttttggttc gcctctcgag ccgatagccg
     5341 ctgctcggcc gccttcgcga cgccgaattc atcaatccca cggcttccct gtcatgacgg
     5401 cgggtctact tggggccgcg tcgcaatagg gccgttaaga agtgaccgcg caaaaacgca
     5461 cgcgccggaa atacatcgag caatcgcagg cggagctgca tgcgatagaa ctgttctgca
     5521 tcccgctcac gaactctgga gcaactgccg ccgaacctac ggcgaggaca atcccttctt
     5581 tcgtttgttc gggcgctcgg ctcccaccca gcgcggaatc gatcgcccct tcaagcgatc
     5641 ggcctggcgg atcgcgaatt gatatccgcc tgacgcgacg acagtaaacc caaagagcac
     5701 cgaaaaactc caggaggtgt tgttgctggc gaaccggtcg tttggcgccg aggcgtcacc
     5761 gtcacctgat cgagtcagga cacgagcatc agtcaggtcg agtgtggcct cgacgtccgg
     5821 ttcgctggtc ggtggcaatg gtcggacatt tgtagcgtca ggcgccgggg gttgatcacc
     5881 ttgcggtgcc ggcggtgact cgggagcatc gaccggaggc gccggcggcg gcgcgggagc
     5941 gtcgaccggc tgtgccggcg acacgtcggg aacattaacc cgagtggcgg cgggcgaggg
     6001 atcgatattg tcgatcaacg acagactcag ccgattggcg tttattcctt ccaagtacaa
     6061 acgccggccg ccgaaagggt caccctgtcc aaagtgagta gccgacgggg gttgtgcatc
     6121 aaggttcgac agcgaggtgc tgttcgacac cgttgtcggc ggcgtggtac tcgggcccag
     6181 ttgtagcacg ggaggcgcta gcggcggcgg aatcacgaag accgtaacgt ccggctgcgg
     6241 ttccgtgctt gatgccgtga tcgatgtatc gttcgggttc aacggagtga ttgtcgagct
     6301 ggtgacggga gataatccgg ccccgttgtc gcccgcgttg acctcatttc cgttcacgtt
     6361 tcccgttacg aaaatgggca cattcgccga attagtcgag ttggccgttc cgaccgaaaa
     6421 tatcggactc agaccgccag acgtcgcgac tccagatacc gagctgtttg gaaggccgga
     6481 cggagtggaa ttggacacag ccactggcgc ggaaaccagg gcaaatgccg tggtagggct
     6541 cgccagcacg ctcgcgccaa gactggtctg accgatcgaa aggagttgac gagtttcgag
     6601 gggttcgcag tgaagt
//
LOCUS       DATAQL010000196         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3685756, whole genome shotgun sequence.
ACCESSION   DATAQL010000196 DATAQL010000000
VERSION     DATAQL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3685756"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VHS97_03290"
     CDS             complement(<1..1258)
                     /locus_tag="VHS97_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447248.1"
                     /translation="MQPTSHLTAPTSTESVGRPLEPEAYPHARYAWYVVGVLTLAYIF
                     SFIDRQILALMVGPIQHDLGIGETQMSLLMGASFAVFYTLFGIPLGRLADTRSRRAII
                     SVGIAVWSVMTAGCGLARTFWQLAVMRMGVGVGEASLSPSAYSLISDYFRPQRRSTAM
                     SVYSMGIYIGSGLAFILGGFITHLVSGRESTDLPWLGPVRSWQLVFFAVGLPGLLISL
                     LLLTVREPVRKGVRLQADGSAITAPSSSAVWSYIRLNSLTFLCLTVGTGLIALYGYGS
                     YSWIPSILIRRYAWTQAKTGLVFGTIVAIGGTLGIVSGGRLADWLRQRGYHDSDLRIA
                     LGGVIAGLPFVVLFPLAPSANWAAGLLVPVVFFMSMPFGVAPAAIQQMMPNTMRAQAT
                     AFYLFVINLLGIGLGPTIVAALTEDVF"
BASE COUNT          354 a          463 c          486 g          263 t
ORIGIN      
        1 ggaaaacgtc ttcggtgagc gcggcgacaa tggtcgggcc gagcccgatg cccaggaggt
       61 tgatcacgaa cagatagaat gccgtcgcct gagcgcgcat tgtgttgggc atcatttgtt
      121 gaatcgcggc cggggcgact ccgaacggca tgctcatgaa gaaaacgacc ggcaccaaga
      181 gcccggccgc ccagtttgct gacggcgcca gcggaaacag cacgacaaac ggcaggcccg
      241 cgatcacgcc cccgagcgcg atccgaagat cggagtcatg ataacctcgt tgccgcagcc
      301 aatccgcaag gcgacccccc gagacaatgc ccagtgttcc accgatagcg acgatggtcc
      361 cgaaaaccag acccgttttc gcttgcgtcc atgcgtaacg gcgaatcaaa atcgatggta
      421 tccacgaata ggagccataa ccgtataggg cgatcagacc ggtgccgaca gtgaggcaaa
      481 ggaaggtcag gctattcagc cgaatatagg accatacggc acttgagctg ggtgcggtta
      541 tcgcagagcc gtccgcttgc aggcgaactc cctttcgaac cggttcgcga accgtgagga
      601 gtaagagcga gatcaggagg ccggggaggc ccaccgcaaa aaagacgagc tgccacgacc
      661 gcaccggtcc gagccacggc aagtcggtgc tctcccgccc ggaaacgaga tgcgtgatga
      721 agccgcccag gatgaacgcc agaccggagc cgatgtagat gcccatggaa taaacgctca
      781 tggcggtgga gcggcgctgc gggcggaagt aatccgagat gagcgagtag gccgaaggag
      841 aaaggctcgc ctcacccacc ccgaccccca tgcgcatgac ggccagttgc cagaatgtcc
      901 gggccagacc gcagccggcc gtcatcacac tccataccgc gatgcccacc gaaatgatcg
      961 cgcggcgact gcgcgtgtcg gcaagccgcc ctaaggggat gccgaaaagc gtatagaaga
     1021 ccgcaaagct ggctcccatc agcaggctca tttgcgtctc gccgatcccc aggtcgtgct
     1081 gaatagggcc gaccatcagg gcgagaatct ggcggtcgat aaacgaaaaa atgtaagcta
     1141 gcgtgagcac gccgacgaca taccaggcat atctggcgtg gggatacgct tccggctcga
     1201 gcggtcggcc gacggactcc gtgctcgtag gcgctgttag gtgtgacgtc ggttgcataa
     1261 gattctctcg gtatctggcg ccgtcgccgg tccgttctgt cttcacgatc ggctgatggc
     1321 ggaatcggaa ctgtcgggtt ttcgtaacgt cggctgccgc tctaggccta acacggtccg
     1381 cgattgtcgc actcagcgaa ggtttgggca atctggcggc tggatcgctc aggacgagcg
     1441 gccggcctgg gcctctcgcg aaattcttcg agttcgggtc gcttctcagt ccgatcaatc
     1501 gcggataaag tcaatgaatt aagtcaccaa tcgcagtcaa cctgtgctcg ccgtgggagc
     1561 tatcga
//
LOCUS       DATAQL010000197         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_631930, whole genome shotgun sequence.
ACCESSION   DATAQL010000197 DATAQL010000000
VERSION     DATAQL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631930"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VHS97_03295"
     CDS             complement(<1..182)
                     /locus_tag="VHS97_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447249.1"
                     /translation="MGASRRDFLRSSCAGALAWNALGLPPGTTRAGAAAAEMGEDERA
                     KLAATALEIARSQGAS"
     gene            471..1250
                     /locus_tag="VHS97_03300"
     CDS             471..1250
                     /locus_tag="VHS97_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX3447250.1"
                     /translation="MNVVVTGGGTIAPIDDVRLMTNVSSGRFAAAISEACLHRGANVW
                     HIHSLTAQIPLRRFAEFALDAIDNASELGRLAQLRERWLKERDRLNLIPLKIGNVADY
                     ATTLEHVLRSHPIDVVFLPMAVADFEPEPVPGKISSESESLILRCRPTPKVIRLVRDW
                     SPSVYLVGFKLLSRVSREELVRRAEVACRDNRADLTVANDLQTLRQGQHTVHLVRPGN
                     DSETLEPGPDLAERLVARVLIWANEPRPAVPASSVVAAEKA"
     gene            1247..2428
                     /locus_tag="VHS97_03305"
     CDS             1247..2428
                     /locus_tag="VHS97_03305"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3447251.1"
                     /translation="MNDPALDQSGENPAPPEAAERIYFPELDGLRFVAFMMVYLFHGG
                     VPQPIASQVIGRSAAAALRENGGFGVQLFFILSGYLIATLLLREEARYGRISLRAFWI
                     RRILRIWPLYYLVIAIGFGLIPAIEGQIMTQGYRDMLRNHLIPFLAFLGNWSMALVTP
                     LPDWLSILWSVCVEEQFYLIVPLLIAFVARKYRVPLVATLIAGSIGVRSFCAHRYGSD
                     LMIVFNTFAQFDTLLSGVLLALCLGWDRDRPRLARWLRWLQWPLYLVFGWLLCQPHLA
                     HGTPLPRTWDYVWVWLCGVGIVIVAVWGGGWLRAALSYSRVVWLGKISYGLYMYHEIA
                     LWTRERMYYLLPWFPNKEELMTIATLALTIAMAAASYYGYERRFLELKRAWTKVPSRP
                     V"
     gene            complement(3036..3476)
                     /gene="tnpA"
                     /locus_tag="VHS97_03310"
     CDS             complement(3036..3476)
                     /gene="tnpA"
                     /locus_tag="VHS97_03310"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX3447252.1"
                     /translation="MANTLTNLLYHIVFSTKDREPLIQATFRDELEKYIAGIVRNEGG
                     IVLGIGGMPDHLHLVAKFRPDRSVAEMARLIKANSSKWVNENHGNPGRFAWQSGYGAF
                     SVSQSQLNGLLAYVANQDAHHHVRSFQDEYREFLIKHGIEFDER"
     gene            complement(3744..>3990)
                     /locus_tag="VHS97_03315"
     CDS             complement(3744..>3990)
                     /locus_tag="VHS97_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3447253.1"
                     /translation="YHASSDDPPTLIIHGDADLLVPIQQAELVVEKLKSSGVETKLIV
                     KKGAAHGWPDLLKDTAIIADWFDEHLKPQKKDSGAGS"
BASE COUNT          679 a         1184 c         1219 g          908 t
ORIGIN      
        1 taagacgcac cctgtgagcg ggcgatctcc agggcagtcg cagcgagctt ggcgcgctcg
       61 tcctcgccca tctcggctgc cgccgcgccg gcccgagttg tccctggtgg caagcccaga
      121 gcattccagg ctagagcgcc ggcgcacgaa ctgcgcagga agtcccgtcg tgaagctccc
      181 acggcctact cctcgacaag tgatgacacg atgcgtaata gactgtcgaa tcgagcggat
      241 tagacggtac tatatcggcg cggcgcagct ccttcaatcg cgtggcggaa atgagcgagt
      301 tcggacccag tgaccggcca actttcgctg gttggcggag atttgggctg ctcggggcgg
      361 gtctttgtcc ttggcccgcc gcactgttgg gcgcgggtct ctgaccccgc ccacgggacg
      421 accgcaggtc tcccgacgcc cctcggtctc tttcgcggag ctagtctttg atgaacgtcg
      481 tcgtgaccgg aggtggcacg atcgcgccga tcgacgacgt acgtctcatg acgaacgtct
      541 cttcggggcg gtttgccgcg gcgatctcgg aagcatgtct gcaccgcggg gcgaacgtct
      601 ggcacatcca ctctttgacg gcccagattc ctctgcggcg ttttgcagag tttgcactcg
      661 acgcgatcga caatgcctcc gagctcggtc gtctggctca gcttcgagaa cgatggctga
      721 aagagcgcga ccgcctgaat ctcattcccc tcaagatcgg caacgttgcc gattatgcca
      781 caaccctcga gcacgtactc cgttctcatc ccatcgatgt cgtctttctg ccgatggcgg
      841 tcgctgattt cgagcccgag ccggtgccgg ggaaaatcag ctctgaatcc gagtcgctca
      901 tcctccgctg ccggccaacg cctaaagtca ttcgcctggt tcgcgactgg tctccctcgg
      961 tctacctggt cggcttcaag ctcctctcac gcgtgagccg cgaggagctc gtgcgccggg
     1021 cggaggtggc ctgccgcgac aatcgcgcgg atctcaccgt cgcgaacgac ctccagaccc
     1081 tgcgccaagg tcagcacact gtgcacctgg tgcgccccgg taatgactcc gagacgctcg
     1141 aacctgggcc ggacctggcc gagcggctgg tcgcgcgcgt gctcatctgg gcgaatgagc
     1201 cgcgaccagc cgttcctgcc tcctctgttg tggcggccga gaaagcatga acgacccggc
     1261 cctggaccaa tccggcgaaa accccgcgcc acccgaggct gccgagcgca tctacttccc
     1321 cgagctcgat ggactccggt tcgtcgcctt catgatggtc tatctatttc acggcggcgt
     1381 gccccagccg atcgcgtcgc aagtgatcgg ccgaagtgcc gcggccgcgc ttcgtgaaaa
     1441 tggcgggttt ggcgtccagc tctttttcat cttgagcggc tacttgatcg ccacgctctt
     1501 gctccgcgag gaagcccgat acggccggat ctctctccgg gcgttctgga tcaggcgcat
     1561 cctgcggatt tggcctctct attatctggt catcgcgatt ggcttcggtt tgatccccgc
     1621 gatcgaggga cagatcatga cgcagggata ccgtgatatg ctccgcaatc atctcattcc
     1681 gtttctggca ttcctcggca actggtcgat ggcgctggtg actcccctac ctgactggct
     1741 cagcattctc tggagtgttt gtgtcgaaga gcagttttat ttgatcgtgc cgctattgat
     1801 tgcgttcgtc gcgcgcaagt accgcgtacc gctcgtcgcc accttgatcg ccggctcgat
     1861 cggcgtgcgc tcgttctgcg cacatcgtta cgggtcagac ctcatgattg tgttcaacac
     1921 attcgcccag ttcgacacac tcttgagcgg tgttctgttg gcgctatgcc tgggatggga
     1981 tcgcgacaga ccgcggctgg cccggtggct gcgctggctt caatggccgc tctacctggt
     2041 ttttggatgg ctcttgtgcc agcctcatct ggcacacggc acgccgttgc cccgtacctg
     2101 ggattacgtg tgggtgtggc tgtgcggcgt gggtatcgtg atagtcgcgg tgtggggcgg
     2161 aggctggctt agggctgccc tgtcgtactc acgagttgtt tggctgggaa agataagtta
     2221 tgggctgtac atgtaccacg agattgcgct ttggaccagg gaacgaatgt actacttgct
     2281 accctggttt ccgaacaagg aggaactgat gacgatcgcg accctggctc tgacgatcgc
     2341 catggcggca gcctcttact acggttatga gcggcgattt ttggaactaa aacgagcctg
     2401 gacgaaggtc ccgtcgcggc cggtgtgaca cgctcccgaa gtatgagcga gttggattca
     2461 cgagcctgag tagggcggaa tcgaatagct cggggcgtta gctttgaggt tctggttttc
     2521 atttgatgag ccccgtcggg gcggaatcat atagctcggg cgtcagcccc gaggtgcggc
     2581 gccagccgcg tgaatgtgcg aacgacctta ccttcctcgt ttttgcattt gacgagcccc
     2641 atcggggcgg aatcgtatag ctcggggcgt cagccccgag gtgcggcgtc agccccgagg
     2701 tgcggcgtca gccccgaggt gcggcggcag ccccgaggtg cggcggcagc cgcgtgaatg
     2761 tgcgaacgac cttacgttcc tcgtttttgc atttgacgag ccccatcgat ccggacatat
     2821 gtcgtgccgc tggacaacat atggtccgga tcagccttct gaaacatatg tccggcccgg
     2881 ctttttggag tgcgaaggag cggtcagaca tatgtctaga aggtaggggc ggaatcgtat
     2941 agcttggggc gttagccccg aggaggagga ggagaacccg tttcctcaag gtcacattcc
     3001 ggcgcccatc tttgattcct cctttacccc cacagttatc gctcgtcaaa ctcgataccg
     3061 tgtttgatca ggaattcgcg atattcgtct tgaaacgatc ggacatggtg gtgtgcgtct
     3121 tggttagcga catacgccag aagtccgttg agctgcgatt ggcttaccga aaatgctccg
     3181 tacccggatt gccaggcgaa ccggcccgga ttcccatgat tttcgttgac ccatttggag
     3241 gaatttgcct tgatcaagcg agccatttcg gcgaccgatc gatccggtct gaatttcgcg
     3301 acgaggtgaa gatgatcagg cattccgcca attccaagga cgattccacc ttcgttccgg
     3361 acaatgccgg cgatgtattt ctcgagctcg tcacggaacg ttgcttggat gagcggttcg
     3421 cggtctttgg tgctgaagac gatgtggtag agcaggttgg tcaacgtgtt ggccatgacg
     3481 tgggcttctc gggggatttc cggtccaccc agacaaatct acgcggttca cgattctctg
     3541 ggggggttcg caattctccg ccgcttgcaa ttctccgggg gttcgcaaat gctctccggg
     3601 ggtttgcacc cccggctcta tgatgtcgcc cctagcgggg ctcgggagga accgctctga
     3661 catccgcgat catcctccgg gggtttgcac ccccggctct atgatgtcac cccgggcggg
     3721 gctaatacgg ggcgaagtcg gcgtcacgat cctgcgccac tgtctttctt ttgtggcttc
     3781 aagtgttcgt cgaaccaatc ggcgatgatg gccgtgtcct tcaacaagtc gggccatccg
     3841 tgtgcggctc ccttcttgac gatcagtttg gtctcgacgc ccgacgactt gagtttctcg
     3901 acaacgagct cggcttgctg gatcggcacc aataaatcgg cgtcgccgtg aatgatcagg
     3961 gtgggaggat cgtcggacga cgcgtgatag
//
LOCUS       DATAQL010000198         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_174028, whole genome shotgun sequence.
ACCESSION   DATAQL010000198 DATAQL010000000
VERSION     DATAQL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174028"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            771..2243
                     /locus_tag="VHS97_03320"
     CDS             771..2243
                     /locus_tag="VHS97_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3447254.1"
                     /translation="MISATAAHSSHKITPRVLADLVAILGVDNVLHQRDETLVYECDG
                     YLVEKSLPDVVVFPTSTEHVVEVVKLCNRHEIPFVPRGAGTSLAGGTLTIGGGLMICL
                     TRMKRILEVSVRDRYALVESGVVNVWLTRSLSGKGFHYAPDPSSQGACTIGGNVATNS
                     GGPHTLKYGVTVNHIKGTTLVMPDGTVVETGGVTEDNPGYDLTGLVVGNEGTFGIVTR
                     AVVNLTRDPEAGRTLLAVFDSVDAATETVSGIIAAGIVPAALEMLDNLMIRAVEQAFG
                     FGFPVEAGAVLIIEVDGLDAGLDREARAISEIVQTHGGSVGKSITWRTRKEPEYVAIW
                     KSRKSAFGAIGRLSPTYCTQDGVVPRTKLPHILRHIVDVGRRHNLRIANVFHAGDGNI
                     HPILLFDERDADQVKRVFLASHEILDECIAVGGSVTGEHGIGVEKMEFMPKLFSPEDL
                     AAMVAIRNTFNPLGRCNASKTLPGGAGCIERKTPGRAASA"
     gene            2416..>3318
                     /locus_tag="VHS97_03325"
     CDS             2416..>3318
                     /locus_tag="VHS97_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447255.1"
                     /translation="MAKRPRFSRWRRLPGVFRTGENRPTDSGNELDRIILYVPARILD
                     LAEVLADKAGVPTVQDYCALLLMQALESERVKRKVSDFEARRGPLEGLKQIADDIHYL
                     AEWQEQSGSRAEAPSPGRAGNHDPAGKNPPTVNEPITVDIALAEGNVRQLESAPDKDR
                     PDVAEEEEKRDQPPATNVEPVAGVIPIFSLETTVVKMTDSGAVDVVLRHAGSGEDQWG
                     FLPCLRRGEPVPAVKVAELNYALNQLEGELKGANTVERRTAHALYRLALEAQVLLTDA
                     WPGVFDERIITAIRTVQEAVERILS"
BASE COUNT          665 a          995 c         1009 g          649 t
ORIGIN      
        1 ctcgtacagg gtcttgagcg cggcgtggta ctgggacttg agggctgatt gcacattcat
       61 ggctctctcc gatgacagtg gaattcatgc cggaacttac ccggcaaatc gtacgggttg
      121 tgagcggagt ccaaggcgca cctgaattag agcggcgtcg tcgcggactg tcaacatgcg
      181 tacagtaaat tcatttccgt caactggcgt tcgcagccga atcccgatta cgatcgcact
      241 ggctgctttt cattggtcga cgatggtcat gtcgccgcgg ccatctcgaa agcgcgaacg
      301 aaccacgacc tggtgaggtt ccctgggcca aacggccaat acgtttgcca tctaaagaat
      361 ccgttgtcaa ccgcgtcgcg cgggatgaga cggccggaga ccttcccaaa aactgccatc
      421 tactgccatc tgctgcagtg ttggaggcgc aaaaccgcta cgccccttgc aaccgttcca
      481 ttccgttccc tgatctcgag ggcatgctgg ccagcctggg cccggggctg agaactttga
      541 gcggggtcaa caagcgcctc ggtggcatgt gcctcttaat cttactcttc gttggagctg
      601 cactcggtcg ttttttacca ctcaatcaaa gggttgacga cccgctcgtg ctttcgtttc
      661 ccgttcaccc aaaggcagaa cgggtttgtc tctggagtct gaccctatcc ataatagcca
      721 gagaatgatg taattcctcg gcatcgccct tccgcgagaa acctgatccc atgatctcag
      781 caactgccgc ccattcgtct cataaaatca cgccgcgcgt gctggcggac ttggtcgcga
      841 tcctgggtgt ggacaatgtt ctgcaccagc gcgacgagac cttggtctac gaatgcgatg
      901 gctatttggt cgaaaagagc ctgcccgatg tggtcgtctt cccaacgtcg accgagcatg
      961 tcgtggaggt cgtcaagctg tgtaaccgcc atgaaattcc gttcgttcct cgaggggccg
     1021 gtaccagcct ggcgggaggc acgctgacga tcggcggcgg gctgatgatc tgcttgacgc
     1081 gaatgaagcg gatcctcgag gtgagcgtcc gcgatcgcta cgcgctcgtc gagtcgggag
     1141 ttgtcaacgt ctggctgacc cgctcgctct cggggaaagg gtttcactac gcgcccgacc
     1201 cttccagcca gggcgcctgc acgatcggcg gcaacgtcgc gaccaactca ggagggccgc
     1261 acacgctcaa atacggcgtg accgtcaacc acatcaaggg gacgacgctg gtcatgcctg
     1321 acggcacggt cgtcgaaacc ggtggggtca ccgaggataa ccccggctac gacctcaccg
     1381 ggctggtcgt gggtaacgaa gggacctttg ggatcgttac ccgagcggta gtcaacctga
     1441 cgcgcgaccc cgaggcgggg cggactcttt tggccgtctt tgactcggtc gacgcggcaa
     1501 ccgaaaccgt cagcggaatc atcgccgccg gaatcgtgcc ggccgcgctc gagatgcttg
     1561 acaacctgat gatccgcgcg gtcgaacagg cctttggctt cggctttccc gtcgaggccg
     1621 gggccgtgct gatcatcgag gtcgacgggc tcgacgctgg gctcgatcgc gaggcccgcg
     1681 ccatcagcga gatcgtccaa acgcacggcg gatcggtcgg caagtcgatc acctggcgga
     1741 cccgcaaaga gcccgagtat gtcgctatct ggaaaagccg caagtcggcc ttcggcgcga
     1801 tcgggcggct ttcaccaacg tactgcaccc aggatggggt ggtcccgcga accaaactac
     1861 cccatattct ccggcacatc gtcgacgtcg gcaggcggca caatctgaga attgccaacg
     1921 tttttcacgc cggcgacggc aatatccacc cgatcttgct cttcgacgag cgcgatgctg
     1981 accaggtgaa gcgtgtgttc ctggcgagcc atgaaatttt ggacgagtgt atcgcggtcg
     2041 gcggcagcgt caccggcgag catgggatcg gggttgaaaa gatggaattc atgcccaagc
     2101 tgttttcacc cgaagatctg gccgccatgg ttgcgattcg aaacacgttc aatccattgg
     2161 gccggtgcaa cgcgtcgaag acgctcccag ggggtgcggg ctgcatcgag agaaagaccc
     2221 cggggcgagc ggcatccgcc tgatctgttt ttcgaaacgg acatcagcac gcccacggcg
     2281 acattcacgg ccaaggtcga atgggttatc ctggagccat atggatccac catcgctttg
     2341 ggatcgttgc caattggcga actggcctca agtggcgaaa cggcctcggt tctgcgtcaa
     2401 cggagaattt ccaacgtggc caagcgaccc cgattttcgc gatggcgacg gcttcccggc
     2461 gtgtttcgga cgggcgagaa ccggcccacc gacagcggga acgaactcga tcgcatcatc
     2521 ctctacgtgc cggcccggat tcttgacctg gccgaggtcc tcgccgataa ggcgggcgtg
     2581 cccacggttc aagattactg tgccctcctc ttgatgcagg ccctcgaaag cgagcgggtt
     2641 aagcgtaagg tttccgattt cgaggccagg cgaggtccgc tcgaaggatt gaagcagatc
     2701 gccgacgaca tccattatct ggccgagtgg caagagcaat ccggcagtcg ggcggaagcc
     2761 ccgtcgccgg gcagagccgg caatcatgat ccggccggca agaatccacc gacagtcaac
     2821 gagccgatca ccgtcgacat cgcgctcgcc gaggggaacg tccggcagtt ggaatcagcc
     2881 cccgataagg atcgacctga tgtcgcagaa gaagaagaga aacgcgatca accgccagcg
     2941 accaatgtcg agccagttgc cggcgtcatt ccgatttttt ccctggagac aacggtggtg
     3001 aaaatgaccg actctggcgc cgtcgatgtc gtcttgcgcc atgccggctc gggcgaggat
     3061 cagtggggct ttcttccctg tctgcgccgc ggtgagccag ttcctgcggt caaggtcgcc
     3121 gaactcaatt acgcgctcaa ccaactcgaa ggcgagctca aaggagcgaa cacggtcgag
     3181 cggcggacgg cccatgcatt gtacaggttg gctctcgagg cgcaggtatt gttgaccgac
     3241 gcatggccgg gcgtcttcga tgagcgcatc atcacggcca tccggaccgt gcaagaagcc
     3301 gtcgagcgta ttctctcg
//
LOCUS       DATAQL010000199         6215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1090331, whole genome shotgun sequence.
ACCESSION   DATAQL010000199 DATAQL010000000
VERSION     DATAQL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090331"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /gene="ruvB"
                     /locus_tag="VHS97_03330"
     CDS             complement(<1..959)
                     /gene="ruvB"
                     /locus_tag="VHS97_03330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX3447256.1"
                     /translation="MREPTIKGNSSDAQGGGRRGGSSSEPDALKPAADAVDDKLRPQR
                     LSEIIGQRAVAERLEISLAAAQKRGEPLPHILFDGPTGVGKTTFATVLHNELGVELNI
                     TSGAALDKKMDVMPFLTNASEGSILFIDEIHRLPRAVEEFIYPVMEDFRVDVVLGEGM
                     SARTINLPLKKFTIIGATTRSGMLSAPLRERFGMHEHLEFYDVDDLARIITINAGKLR
                     SNLVDKAAWELAARSRGTPRIANARLRWVRDYALARADGHISLSVARDALDMQEIDSE
                     GLDKQDRRYLETLIRVFAGGPTGVEALAATMNVSVDTLKDEVEP"
     gene            complement(1052..1708)
                     /gene="ruvA"
                     /locus_tag="VHS97_03335"
     CDS             complement(1052..1708)
                     /gene="ruvA"
                     /locus_tag="VHS97_03335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX3447257.1"
                     /translation="MLTKVRGILETLSDESLTLSVDPFEIEVLIPEHTRRQVQAKLGQ
                     PITLHTILYIEGGAMASRLVPRLVGFLSAIDREFFEIFCSVDGVGVRKALRAMVLPVR
                     ELARTIQDQDLKLLSTFPGIGEATAERVVAKLRRKVGKFALIVRPETTAAATTETNGA
                     QEVAEPDVIRDTYAALLSVGHSDSEAREAIDRVMTGKKRFKSVADMIEAIYLQGRQKG
                     "
     gene            2899..3621
                     /locus_tag="VHS97_03340"
     CDS             2899..3621
                     /locus_tag="VHS97_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3447258.1"
                     /translation="MDDASLSKTQWIRLIALILVVAAAASTGCSTYIGTTSKSFLHQV
                     RDNPDPNIRYIAYSKLGVPSAYDNQAQKDEAIGIMIAKLREAKEPVAVRAVIVRSLGN
                     LGDRRARQEIIKAANDVENAVIRVEACRALGKVGQPEDATILARIMTIDKLEDCRIAA
                     IEGLGMLKAQDPRIFNILLDGMDHDDPAIRLECLRALREITGKDAGVDPAAWRRELEP
                     TLKELAAKSVSPGTAALKSTPR"
     gene            3865..5997
                     /locus_tag="VHS97_03345"
     CDS             3865..5997
                     /locus_tag="VHS97_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA strand exchange inhibitor protein"
                     /protein_id="HEX3447259.1"
                     /translation="MDTHTLELLDFDKVRALVAARAACSLGKSAAARIAPTIDRGQIH
                     DWQALTTEMTEAVGAGLKPSFGGLHDIRELARRAHRGSVLEPEELAETVETLRAIGSL
                     DVWLARVGDQFPRLGYLRQSVGEFSGVAVAIEGCLDSRGTVLDTASRRLSGLRREIGR
                     VQDRIQETLRQMLRSPAVRRTLRFPNFTMVGHHYVLPVAKDHRGEVQGSVLRSSASND
                     TVYVEPTAIGEQSAQLSFLRAREAKEMRRILRWLSAQVGMVAEALLQTLDTMAELDLV
                     HAKARLSMDYRMSAPQFNDEGRLVLRAARHPLLEALFRGERSMPVSPDPSSTVADDSD
                     RAGAGTEFVAPIGHSSVRPDASVATPAPSPDSRTVTPIDVNLGARFHILVVTGPNTGG
                     KTVALKTVGLLAVMAQSGLHIPAGEGSELPIFDDVLTDIGDEQSLEQSLSTFSSHVGR
                     IREILGRATERSLVLLDELGAGTDPTDGAALGRAILDELDRVGCRSIVTTHIGDLKTY
                     ALSNPDAENAAVEFDDETLKPKYRLMIGDIGHSNALKIARQLRLPEHVVARAESYLAQ
                     NRSGGVPEWEVLQQVRKEAEQARDASMRVQAEAERTRAALVQRLAELQDESRKAEEIV
                     EARARLQAGDRVVVPRLGYDRPGRLVKLDPRKKTAVVSIGHVTWNVSIDELIPQSTRA
                     SSQDSPRSSATAPKNSGARPATLPEDDE"
BASE COUNT         1205 a         1853 c         1917 g         1240 t
ORIGIN      
        1 ggctcgactt cgtctttgag ggtgtcgacc gacacgttca tcgtggcggc cagagcctcc
       61 actccggtcg ggccccccgc gaagacgcgg atcagagttt ccagatagcg gcggtcttgc
      121 ttatcgaggc cctcggagtc gatctcttgc atgtcgaggg cgtcgcgcgc gaccgacaga
      181 ctgatatgcc catcggcgcg ggccagcgcg tagtcgcgga cccagcggag gcgcgcgttg
      241 gcgatgcgcg gggttcctcg gctgcgggcg gccaactccc atgcggcttt gtcaacgagg
      301 ttcgagcgca gcttgccggc gttgatggtg atgatccggg cgaggtcatc cacatcgtaa
      361 aactcgaggt gttcgtgcat gccgaatcgc tctcgaagcg gagcagagag catgccgcta
      421 cgtgtcgttg cgccaatgat cgtgaacttc ttgagaggca gattgatggt gcgcgccgac
      481 atcccctcgc cgagcacgac gtcgacccga aagtcctcca tgaccggata gatgaactcc
      541 tcgacggcgc ggggcagtcg atggatctcg tcgatgaaca ggatcgagcc ctccgaggca
      601 ttggtcaaga aaggcatgac gtccatcttc ttgtcgaggg ccgcgccact cgtgatgttg
      661 agctcgacgc ccagctcgtt atgcaacacc gtcgcgaaag tcgtcttgcc cacgccggtg
      721 gggccgtcga aaagaatgtg gggcaggggt tcgccccgtt tctgcgcggc ggccaaactg
      781 atctcgaggc gctcggccac cgcgcgttgg ccgatgatct ccgagagacg ctgtggccga
      841 agcttgtcgt cgactgcgtc ggcagctggt tttagagcgt ccggttcgga agaagaaccg
      901 ccgcgccggc ctcctccttg cgcgtcactg gaattccctt tgatcgtcgg ctctcgcatc
      961 gatccgtcct ttcaagaagt cagatcggaa ttcgggtttc gacttcttct ctcttctttc
     1021 tatctttctc tgttttcttg gttaactctc ttcatccctt ttgccggcct tgcagataga
     1081 tggcttcgat catgtcggcg acggatttga acctcttctt tccagtcatg acgcggtcga
     1141 tggcctcgcg tgcctcgctg tcggaatgac ccactgagag aagcgcggcg taggtgtcgc
     1201 ggatcacgtc gggctcggcg acttcctgtg cgccgttggt ttccgtcgtg gcggctgcgg
     1261 tggtttcggg tctgacgatc agggcaaact tgccgacctt gcggcgcagc ttggcgacga
     1321 cgcgctcggc cgtcgcctcg ccaatgccgg gaaatgtaga gaggagcttc aggtcctggt
     1381 cttgaatggt gcgcgccagt tcgcgaaccg gcaggaccat ggcccggagc gccttccgta
     1441 cgccgacgcc gtcgaccgag cagaaaatct cgaagaactc gcgatcgatg gcggacagga
     1501 atcccaccag ccgtgggacc agtcggctgg ccattgcgcc gccctctata tagaggatgg
     1561 tgtgaagcgt gatcggctgg cccagtttcg cttggacctg ccggcgggtg tgctcgggaa
     1621 tcaacacttc aatctcgaag gggtcgactg agagcgtcag tgactcatcg gagagcgtct
     1681 ccaagatacc gcgaactttg gtgagcactc ttgcttaccg tcctttcgaa aaccgatcat
     1741 aaccaagcgc tgataccttt gttcagacat tctgccgcaa cgttctaggg gttgcaaggc
     1801 gagtcgaatg gctctggtgt cgcacgccaa ggacgcggtc actcgatcga ttcgatcggc
     1861 gcggagagaa aaatcagaaa ttcccaagtg tctcaccaag atttttcgac aagattttgc
     1921 tgcggcgtga aatttctttt cgcttgctag aacatacact tctccgtgtt tgacatgctt
     1981 cgtgatcatc acacgaaggc gtcggtctgt tgccataagc tctgaaaaaa aagggaaaaa
     2041 ccgcgataat ctgacaagta taatgcgatt tagtatttaa tataaaaact atgaattgct
     2101 aactacaata tgatccagct taatcatcca ctgcaaatcc catctcttcg gcaaaaaaaa
     2161 cgaccatttc tcgcgaagtt cctcttccaa agtttaaaag atttggtatg aattggcgca
     2221 gggaccggtc gattcatgcc ggtcggactc aagcaagtat gtctttgaac aacgaggtga
     2281 tgtcgatggc caagaaacct gaaccgaaga agaccaccgc tgccaagact gctccggccc
     2341 cgaagaaggc tgcccccaag aaggccgccc ccaagaagaa gtgaggccgc tggccgccgc
     2401 cgtgtttcgc ggcggcggcc aagccccgcg ccctacggca aaaaagggcg ctcccgccaa
     2461 ccaacgcaca acaggatgtt cattcggtgg ttccatggaa acgcgtgggg ccggcccttc
     2521 ttggcaacga ggccaaggcc aacttgcgag tcgtccggct cccattaaga tttggatgta
     2581 atccggggcg agatcgacgg agtgtgtcgc cccattgtga ccgagcttgg tctaaagcat
     2641 cgaggaacgg cgcgcttcat gttgcccgga tggctggttc ttttgttctg gcttgccgca
     2701 ccgagctgcc cttggcgaat gccgaggttc tggttaacat gccgccatat catggagcgc
     2761 gtcctcccag tgcgaggcga agtgcgtctc ggctccgata tagaaggaat gcgagctctc
     2821 ggcttcgcat tacaggccgt cgaggtaaaa cgtcgaacgg ccgatcgtta acttgcgcga
     2881 aagacggggc agacggtcat ggatgacgcg tcactctcca aaacgcaatg gatcaggctc
     2941 atcgctttga tattggtggt cgcggccgct gcttcaaccg ggtgttcgac ctacattggg
     3001 accacttcca agagcttcct ccatcaggtt cgtgacaacc ccgaccccaa cattcgctac
     3061 atcgcttatt ccaagctggg ggttccctcg gcctacgaca accaggctca gaaggatgaa
     3121 gcgattggaa tcatgatcgc caagcttcgg gaggccaagg agccggtggc cgttcgcgcg
     3181 gtcatcgtac gaagcctcgg aaatctgggc gaccgtcgtg ctcggcagga gatcatcaaa
     3241 gccgccaacg atgtcgagaa cgccgtgatt cgagtcgagg cctgccgcgc tctcggcaag
     3301 gtcggacagc cggaagacgc aacgatcctc gcccgaatca tgactatcga caagctcgag
     3361 gactgccgca tcgcggcgat cgaaggcctg ggaatgctca aggcccaaga cccgcggatc
     3421 ttcaatattc tcctggacgg tatggatcat gacgatcccg ccatccggtt agaatgcctg
     3481 cgggctctga gggaaatcac cggcaaggat gccggcgtcg accctgccgc gtggcggcgc
     3541 gagctcgaac cgactctcaa ggaactggcc gcaaaatccg tcagcccagg caccgccgca
     3601 cttaagtcga ccccgcgcta aagtggcggg ccgtgctctc acatccttca tgcgcgcttc
     3661 actccgagcg acgagggccc cagaggatcg atcgccattc cggctcaact cgccatggtt
     3721 ggatcggggg caaccttgcg ataatgcgct atctcgcgtg aaccgggcat tcgcctttag
     3781 tgtcggtgag tgcgcgatca cgaagggagg agacccggcc gcccgatggc gtcccgggcg
     3841 cgggcatcgc ggtcgtgagg tcacatggac acgcacacgc tcgagcttct cgacttcgac
     3901 aaggttcgtg cactggtcgc cgcgcgtgcg gcctgctcat tgggaaagag tgcggccgca
     3961 cgcatcgcgc ccaccatcga ccgcggccaa attcatgatt ggcaggcttt gacaaccgag
     4021 atgaccgaag cggtcggcgc gggcttgaag ccctcatttg gcgggttgca cgatattcgc
     4081 gagttggcgc gacgagcgca taggggaagt gtgctcgagc cggaagagct cgccgagacg
     4141 gtcgagacgc ttcgagccat tggcagcctc gacgtttggc tggcgcgcgt cggcgaccag
     4201 tttccccgcc tggggtatct ccgccagagc gtgggtgagt tctccggggt tgccgtcgcg
     4261 attgagggct gtctcgatag ccggggcacg gtgctcgata cagccagtcg ccgactctct
     4321 gggttgcggc gcgagatcgg ccgtgtacag gatcggattc aagaaacgtt gaggcagatg
     4381 ctccggtcac ccgcggtccg gcggactctc cgctttccca atttcacaat ggtcgggcat
     4441 cattacgtct tgccggtggc caaagaccat cgtggcgaag tgcagggctc cgttctccgc
     4501 tccagtgcca gcaacgacac cgtgtacgtc gagcccaccg cgatcggtga gcagtcggca
     4561 cagctttcgt tcctgcgggc gcgcgaggcg aaggagatgc gccgaatctt gcgctggctc
     4621 tcggcgcagg tggggatggt cgcggaggcc ttgctgcaaa ctctcgacac catggcggag
     4681 cttgacctgg ttcacgccaa ggcgcgtctc agtatggatt ataggatgtc ggctccgcag
     4741 ttcaacgacg agggaaggct cgtgctccgc gccgccaggc accctttgct cgaggcgctg
     4801 tttcgtggcg aacgatccat gccggtgtca ccggatccgt cctcgaccgt agcggacgac
     4861 tctgatcgcg cgggtgctgg gactgaattc gtcgcaccga tcgggcatag ttcagtccgt
     4921 ccggacgcca gcgttgccac gccggccccc tcgcccgact cgcggacggt caccccgatc
     4981 gacgtgaatc ttggggctcg atttcatatt ctggtcgtga ccggacccaa caccggcggc
     5041 aagacggtcg cgctcaaaac ggtcgggtta ctggcggtca tggctcagtc cggtttgcat
     5101 atcccggccg gcgaaggctc cgagctgcca atctttgatg acgtcctcac ggatatcggc
     5161 gacgagcaga gcttggagca gtcgctgtcg actttttcgt cgcacgtggg ccggatccga
     5221 gaaatcttgg gccgggcgac ggagcgttcg ttggttttgc tcgacgagct cggggcggga
     5281 accgatccca ccgacggggc ggcgcttggc cgcgcaattc tcgacgaact ggaccgcgtc
     5341 ggttgccgct ctatcgtgac gacccatatc ggcgacctca agacttacgc gctatcgaac
     5401 cccgatgccg agaacgccgc cgtcgagttc gacgacgaaa cgctgaagcc aaagtatcgg
     5461 ctgatgatcg gggacatcgg tcattccaac gcgctcaaga ttgcgcggca gttgcggtta
     5521 ccagagcacg tggtcgcgcg ggcggagtcg tatctggccc agaatcgaag cggcggagtt
     5581 ccggagtggg aggttctcca gcaggttcgt aaagaggctg agcaagcccg cgatgcatcg
     5641 atgcgggtcc aggcggaagc ggagcgcacg cgggcggcgc tcgttcagcg tctggcggaa
     5701 ttgcaagacg agtcgcggaa ggccgaagaa atcgtagagg cacgcgctcg tcttcaggcg
     5761 ggagaccgcg tggtcgtgcc gcgactcgga tacgaccgac ccggcagatt ggtgaagcta
     5821 gacccgcgca agaaaaccgc cgtcgtctcg attgggcacg tgacctggaa cgtctcgatc
     5881 gacgagctca tcccccaatc gacccgtgcg tcgtcgcagg attccccacg gtcgtcagcc
     5941 accgcgccta agaactcggg cgcgcggcca gcgacattgc ccgaggatga cgagtaacga
     6001 acctcgaaag aacaacacgc gcgatcctag acctccgtcg tggcacgggc ttccagcccg
     6061 tgtccgggta gacgacgggc aagatgcccg tgccacttgc tcaatttatt tggttgcgga
     6121 ggcacgcagc gcccctcacc cgccctgcgg gcacactcta cccgtttcgg gcttagcagg
     6181 aggggttttc gtcaccacta gctccctctc cttgg
//
LOCUS       DATAQL010000200         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3379756, whole genome shotgun sequence.
ACCESSION   DATAQL010000200 DATAQL010000000
VERSION     DATAQL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379756"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VHS97_03350"
     CDS             complement(<1..436)
                     /locus_tag="VHS97_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3447260.1"
                     /translation="MGESYTHPEVLVSADWVQEHLKDSKVRVVESDEDILLYDLAHIP
                     GAVRIDWQGDLQDQLIRYYINADKFSALCSKAGIASDTTVIFYGDKSNWWACYAFWAF
                     KLFGHKDCRILNGGRKLWLDQGRPTTTEIPTYPPTKYIVAGSD"
     gene            complement(500..1810)
                     /gene="sufD"
                     /locus_tag="VHS97_03355"
     CDS             complement(500..1810)
                     /gene="sufD"
                     /locus_tag="VHS97_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX3447261.1"
                     /translation="MTSKAAGFTEETFKAFLRDRDEPSWLTERRREAFARFQASAWPS
                     ARDEEWRRTDIRGFKLDGFSPPSPEQPTVAARESFDDQWNALSLHYATGIEHINGSLV
                     REPEPARLGGAVFVDLNRAVKDYPELLKRYLLTEAVTPAADVFTALHSAFWTSGTLLY
                     VPKGVALEAPLFELIGLAQEGRVDLSHTLIVLEDGAQATLVRESAGRGEGLLQALHVG
                     AVEVFLAQGARLRLVNIQNWDEGTWHFSRERALVGRDASLQWTVGGLGARVAKVNQEV
                     ALLGKGADAQVNGVMFTTGRQHLAYFTRQDHVAPHTTSDLLYKGGLKNKSRIVWKGMI
                     RVEKDAQRTNAYQKNDNLILSESARADSIPGLEIEANDVRCTHGATAGRVDDEMIFYA
                     QARAIPRQTAIRLIVEGFFANVYDRITVEPVRETLRQAVATKLA"
     gene            complement(2211..3626)
                     /gene="sufB"
                     /locus_tag="VHS97_03360"
     CDS             complement(2211..3626)
                     /gene="sufB"
                     /locus_tag="VHS97_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX3447262.1"
                     /translation="MSTDLNLQVAGIKDDYKYGFHDSEENYAFKSGKGLTREIVCKIS
                     EMKNEPFWMREFRLKALEVFRQKPTPEWGGDLSTLNFDDIHYYMKAADRQGKSWDDVP
                     AEIKNTFDKLGIPEAERKFLAGVGAQYESEVVYHSLRDDLQKKGVIFVDTDTAVREYP
                     DLVKQYFTTVIPIQDNKFAALNTAVWSGGSFVYIPAGVKVDIPLQAYFRINAENMGQF
                     ERTLIIVEEGAQVHYVEGCTAPMYSSESLHSAVVEIVVKKHGRCRYTTIQNWANNIYN
                     LVTKRAVAHEDALMEWVDGNLGSRLTMKYPSVYMVGKGARGEILSIAFAGKGQHQDAG
                     GKVVHAAPYTSSRIISKSISKNGGRASYRGLLKVAHGAKGCKSNVVCDALILDDRSRS
                     DTYPYIEIDEDDVKIGHEASVSKIGEEQLFYLMSRGLSEAEASTLIVSGFIEPLVKEL
                     PMEYAVEMNKLIQLQMEGSVG"
BASE COUNT          718 a         1145 c         1141 g          878 t
ORIGIN      
        1 catcgcttcc cgccacaata tatttcgttg gcggataagt ggggatctcg gtcgtggtcg
       61 gacgcccttg atcgagccag agtttgcggc cgccgttcaa gatccggcaa tccttgtgtc
      121 caaacaactt gaaggcccaa aaggcgtagc acgcccacca atttgactta tccccgtaaa
      181 agatcacggt tgtgtcactg gcgatgcctg cttttgagca tagcgcgctg aatttatcgg
      241 cgttgatgta gtagcgaatg agttggtctt ggagatcgcc ctgccagtcg atccggacgg
      301 cgccggggat gtgcgccagg tcgtagagca agatatcctc atcgctctcg acgacgcgca
      361 ccttggagtc cttgagatgt tcttgaaccc agtcggctga caccagcact tccggatgcg
      421 tgtacgactc tcccataatg caaactccgt gaaaaagtgg ctgtggaata gatcggccat
      481 gacgcttctt cggtcggcct tacgcgagct tggtcgcgac ggcttggcgg agcgtctcgc
      541 gcaccggctc gaccgtgatt cgatcgtaga cgttggcgaa gaagccctcg acgatcaagc
      601 ggatggcggt ctggcgtggg atcgctcggg cctgagcgta aaagatcatt tcgtcgtcga
      661 ctcgtcccgc agtcgcgccg tgggtgcaac gaacgtcatt ggcctcgatt tccagacccg
      721 gaatcgagtc ggcacgggca gattccgaca ggatcagatt gtcgtttttc tggtaggcgt
      781 tggtgcgctg ggcatctttc tcgacgcgga tcatcccctt ccagacgatc cgggacttgt
      841 tcttgagccc ccccttgtag agcagatcac tggtcgtatg gggggcaacg tggtcttgcc
      901 gggtgaaata agcgagatgc tggcggccgg ttgtgaacat gacgccattg acctgggcgt
      961 cggctccctt gcccaggagg gcgacttcct ggttaacctt tgccacccgc gcgcccaggc
     1021 cgccgacggt ccactgcagg ctagcgtcgc ggccgaccag cgcccgttcc cggctgaagt
     1081 gccacgttcc ctcgtcccag ttctggatat tcaccaatcg caaacgcgcg ccttgcgcca
     1141 ggaagacctc gaccgcaccc acgtgcagcg cttgaagaag accctctccc cgaccggcag
     1201 actcgcggac aagagtggct tgggcgccgt cttccagcac aatgagcgtg tgactcaggt
     1261 cgacccgccc ctcctgggcc agaccgatca gctcgaacag aggggcctcc agtgccactc
     1321 ccttaggcac gtagagcaat gttccgctcg tccagaacgc tgaatgtagg gcggtgaaga
     1381 cgtcggcggc gggagtcacg gcctcggtca gcagatagcg tttcagcagt tcgggatagt
     1441 ctttgaccgc gcgattcaga tcaacgaaga ccgctccccc gagccgcgcc ggttcgggct
     1501 cacgtaccag gctgccattg atgtgctcaa tgccggtggc atagtggagg cttagggcgt
     1561 tccattggtc gtcgaacgac tcgcgggccg cgacagttgg ttgctccggg gacgggggac
     1621 taaaaccatc gagcttgaaa ccgcggatgt cggtccgccg ccactcctca tcgcgcgcac
     1681 tcggccaggc ggacgcctga aatcgggcga atgcctcgcg tcgccgctcg gtcagccacg
     1741 acggctcgtc acggtcgcgg aggaatgcct tgaaggtttc ctcggtgaag ccggcagctt
     1801 tagaagtcaa taaggttccg gatgcactgc tactcattgt gctctgtctc ttcagaggcc
     1861 cagccaggtc cgcacggcgt tttcgacttg agcgagttga actccctcga gatcgccaag
     1921 tttccttacc aatttagcct gggtgatcga tgttggattc tgcgcatcga atacgcccct
     1981 ctggaggaat ggtcgaggtg cggtcacctc gaatcgcgtt cctctgggac tagttgtatg
     2041 aggaatgacg gttactattg cacgatcggt atcaagaacc ggaacgctaa gcaccaagca
     2101 cggccgagtt ttggccacgt atccgagatc gacgatccac acctcgccgc gatcagccct
     2161 ggacatcggc cgcctcgcga gcgtcgtatt caatatcgat cgcctccgat ttagcccacc
     2221 gagccttcca tctgaagctg gatgagcttg ttcatctcga cggcgtattc catgggcagc
     2281 tctttgacga gcggctcgat gaatccactg acgatcaatg tcgacgcctc ggcttccgag
     2341 aggccgcggc tcatcaagta gaagagctgt tcctcaccga tcttggagac gctggcctca
     2401 tggccgatct tgacgtcgtc ttcgtcgatt tcgatgtacg gataggtgtc ggagcggcta
     2461 cggtcgtcga gaatgagggc gtcgcacacg acgttggatt tacatccctt ggccccgtga
     2521 gcgaccttca aaagcccgcg atagctggcc cggccaccat tcttgctgat gctcttggag
     2581 atgatccgcg agctggtgta aggcgcggcg tggacgactt tgccgccggc gtcttgatgc
     2641 tgacctttgc cggcgaaggc gatcgagagg atttcaccgc gagcaccctt gccgaccatg
     2701 tagaccgacg ggtacttcat cgtgagcctg ctacccaggt tgccgtcaac ccattccatc
     2761 agggcgtctt cgtgagccac ggcccgcttg gtcacgaggt tgtagatgtt attggcccag
     2821 ttttgaatcg tcgtataacg gcagcgtcca tgctttttga caacgatctc gacgaccgcc
     2881 gagtgcaggc tttcgctcga gtacatcgga gccgtgcagc cctcgacgta atggacttgc
     2941 gccccttcct cgacaatgat cagggtccgc tcgaactggc ccatgttctc ggcgttgatg
     3001 cggaaatagg cctgcaatgg gatgtcgacc ttgacgccgg cgggaatgta gacgaacgac
     3061 ccgccgctcc agaccgccgt gttgagcgcg gcgaacttgt tgtcttggat tggaatcacg
     3121 gtcgtgaagt attgcttcac cagatcggga tactcgcgaa ccgcggtatc ggtatcgacg
     3181 aagatcactc cttttttctg taggtcgtcg cgcaggctat ggtagacaac ctcggattca
     3241 tactgggcgc cgacgcctgc cagaaacttg cgttcggctt cgggtatgcc caatttgtcg
     3301 aacgtattct tgatttcggc tggtacgtcg tcccagcttt ttccttgacg gtcggcggcc
     3361 ttcatgtagt agtgaatgtc gtcgaagttg agagtcgaca ggtcgcctcc ccattccggc
     3421 gtcggctttt gccggaagac ctcgagcgcc ttcagccgga attcccgcat ccaaaacggc
     3481 tcgttcttca tttccgagat cttgcagacg atctcgcgcg tcaatccctt gccgctcttg
     3541 aatgcgtagt tttcttccga gtcgtgaaac ccgtatttgt agtcgtcctt gattcccgca
     3601 acctggagat tcagatcggt agacatcggc aactatccct tccttcgtga agttgaacct
     3661 gctcgggcgc gacgcggcgg acgattgtcg gaaaggcggt gaacgttagc ccgcggccga
     3721 cagcggcttc tgcggctgtg cggcttccag ttccgcctcg tcccgggctg cgtcggggta
     3781 tttttcgcgg acccattcgt agccttcttt ttcgagtcgt ttgatcagct cggcaccgcc
     3841 gttttccacg atccggccgc cgaagagaat gtggacgaat tg
//
LOCUS       DATAQL010000201         5074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2770062, whole genome shotgun sequence.
ACCESSION   DATAQL010000201 DATAQL010000000
VERSION     DATAQL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2770062"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..761)
                     /gene="hypB"
                     /locus_tag="VHS97_03365"
     CDS             complement(117..761)
                     /gene="hypB"
                     /locus_tag="VHS97_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX3447263.1"
                     /translation="METDVLAKNNRLAERNRGWFAGRNILALNLMSAPGAGKTTLLER
                     TINDLRTELALSVIEGDQETINDAERIRATGCSVVQINTGTGCHLDAVMLAKGLQELA
                     PPMNSIVMIENVGNLVCPALFDLGESAKVVITSVTEGDDKPLKYPHMFRESTVMILNK
                     VDLLPYVPFSMARCLDFVRQVNPRLRVIPVSALRGDGLDEWYDWIRREMKSCSQ"
     gene            801..1022
                     /locus_tag="VHS97_03370"
     CDS             801..1022
                     /locus_tag="VHS97_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447264.1"
                     /translation="MRMCVPVVGLVMIVVVIMVMVVIVVVIVVVMMMSVTIVLGEAGV
                     LVLDVAVISGADRKRLAALKVDELGLGIV"
     gene            complement(1036..1368)
                     /locus_tag="VHS97_03375"
     CDS             complement(1036..1368)
                     /locus_tag="VHS97_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX3447265.1"
                     /translation="MHELGIAQEVVALVADHARGKVTRVVLEIGKLSAVLPDAIRFCF
                     DLCSEGTLVAGADLEIVEIPGLARCRACGGEVALEKPFGRCDCGGSDLEWLSGEELRI
                     KEYEVADV"
     gene            complement(1380..1502)
                     /locus_tag="VHS97_03380"
     CDS             complement(1380..1502)
                     /locus_tag="VHS97_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447266.1"
                     /translation="MKVLETLAGAVIAVLSVVILLVGSIFALGSMGKYMRNKAM"
     gene            complement(1539..2060)
                     /locus_tag="VHS97_03385"
     CDS             complement(1539..2060)
                     /locus_tag="VHS97_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX3447267.1"
                     /translation="MLPRILVAGIGNIFLGDDAFGVEVVRKLVERAWPQNVSVVDFGI
                     RGFDLTMALLEEIDLVILVDAAPRRGPPGSLYVIEPDAGEHDNSAADEPLVDPHSLDP
                     VKVLRMVKTMGAGPKRVLVVGCEPENVGSDLDWDVTPGLSEPVQQAVDRAVELVESLI
                     RDAHLGRLALGAR"
     gene            complement(2063..2374)
                     /locus_tag="VHS97_03390"
     CDS             complement(2063..2374)
                     /locus_tag="VHS97_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447268.1"
                     /translation="MDHWDPTIEQPKLATIRVAGVEFHIGDRVRLWPLGDADILDMAM
                     KGKMARIAAIEQDYEDRVYLAVTVDDDPGQDMGLAGKPGHRFFFRPEEVEPLNDDEPR
                     I"
     gene            complement(2443..3492)
                     /locus_tag="VHS97_03395"
     CDS             complement(2443..3492)
                     /locus_tag="VHS97_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447269.1"
                     /translation="MNQGLVDQIAAAVLYEGYILYPYRPSVKNRQRWTFGGLVPRAYS
                     QLQCVSEPFEMQTECLVAGSPASSITISVRFLQLVSRLVGQFADPLDEWPDEGVIPCP
                     TVELLQVGDEMFYTWQEAVEQRIDMGESSLGALADCPRRREFALASHRDRQPLRWPDG
                     KIVGAIVRERKSIEGQVETSAAEVAPGVFKVTVKIENDTPLDLNNAADRDEALTHGLV
                     STHTVLGVRDGEFVSLIDPPEPYKNVASLCRNVGCWPVLVGDEGDNDTMLSAPIILYD
                     YPQIAPESPGELFDGTEIDEILTLRIMTLTDQEKQAMAAVDQRARAVLARTELLDNEQ
                     MLGLHGTFRGLRPFT"
     gene            complement(3489..4148)
                     /locus_tag="VHS97_03400"
     CDS             complement(3489..4148)
                     /locus_tag="VHS97_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEX3447270.1"
                     /translation="MLDLNFTVETAEVEPHAAAPLLIFKLRIAESVAAEEPTTIPAIA
                     LKCQIRIEPARRRYAADEEEKLLDLFGTRERWGHTLRSTLWTHTSVVVPPFTGETVID
                     LPVPCSFDFNVAATKYFYALADGEVPLCLLFSGTIFYTDRDGYLQVSQIPWEKEASYR
                     LPVQVWKNMMELYYPKSAWLCLERDTFDRLYRYKSSRGLPTWEQAIDMLIDSVLEEVE
                     S"
     gene            complement(4159..4788)
                     /locus_tag="VHS97_03405"
     CDS             complement(4159..4788)
                     /locus_tag="VHS97_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEX3447271.1"
                     /translation="MQPADAPVRNPLSALRQFVRKPSGPPAERCELCGLAIAAGHAHL
                     LEPATRQLVCSCEPCAVLFSGRQVGRYRRVPRDIWALPEFRLTDIQWEDLHLPINLAF
                     FVQSTPAQRVLALYPSPAGAVESLLTLEAWQTLVVDNPVLGELEPDVEALLVNRMKDV
                     RAYYRTPIDECFKLVGLIRAHWRGLSGGTEVWDEVGRYFKSLHERSRTR"
BASE COUNT          956 a         1502 c         1603 g         1013 t
ORIGIN      
        1 cccccaggcc atgacctcgg ccaacggagt ggctcgctga gggtccccgg atcacgaaca
       61 cgcgaccgag cgaaaagtca tcggtcaggc caacgggccc gaccgatgac tttttcctat
      121 tgggaacatg acttcatctc gcggcggatc cagtcgtacc attcgtccag gccgtcgccg
      181 cggagcgcgg agacggggat cacgcggaga cggggattga cctggcggac gaagtcgagg
      241 caccgcgcca tgctgaaggg gacgtacggc agcaggtcga ccttattgag aatcatgacc
      301 gtactctcgc ggaacatgtg cgggtacttg agaggcttgt cgtcgccctc ggtgaccgat
      361 gtgatcacaa ccttggcgct ctctcccagg tcgaagaggg ccggacagac gaggttgccc
      421 acgttttcga tcatgacgat cgagttcatg gggggagcaa gttcttgcag ccccttggcg
      481 agcatgaccg cgtctaagtg acaacccgtg ccggtgttga tctgaacgac actgcagccg
      541 gtcgcacgaa ttcgctcggc gtcattgatc gtttcttgat ccccctcgat gaccgagaga
      601 gctagctcgg tacgcaggtc gttgatcgtg cgttcgagta acgtggtctt gcctgcaccc
      661 ggggcgctca tcaggttcaa ggcgaggatg ttgcggccgg caaaccagcc gcgatttcgc
      721 tcggccaggc ggttgttctt ggcgagcaca tcggtttcca ggcgaactgt tgcggcctca
      781 gaatgagaat gagcgtgcgc atgcgaatgt gtgtgcccgt tgtgggcctc gtcatgatcg
      841 tggtcgtgat catggtcatg gtggtgatcg tagtggtgat cgtggtcgtg atgatgatgt
      901 ctgtgacgat cgtgctgggc gaagccggcg tgctcgtgct cgacgtcgcg gtcattagcg
      961 gtgccgatcg caagcgtctg gccgctctga aggttgacga gcttgggctt ggaatcgtct
     1021 gagcaaccac aggtttcaca catccgccac ctcgtattcc ttgattctga gttcttcacc
     1081 acttagccat tcgaggtcgc tgccaccaca gtcgcaacgg ccaaacggct tttcgagagc
     1141 gacctcgccg ccgcaggcac ggcagcgggc gaggccggga atttcgacaa tttcgaggtc
     1201 ggctccggcg accaaggtcc cctcgctgca caggtcgaaa caaaaacgga tcgcgtcggg
     1261 cagaacggcc gaaagcttgc cgatctcgag gacgacacgc gtgactttgc ctcgggcatg
     1321 gtcagcgacg agcgcgacca cctcctgcgc gattccgagc tcatgcatct agcgaacctc
     1381 tacattgcct tattgcgcat gtacttcccc atcgagccga gggcaaagat cgatcctacg
     1441 agcaatatga ccactgagag aacagcaatc acggcgcctg cgagcgtttc gagaactttc
     1501 atgctacgct cctctcgaat caacgaggcg atcttggctc atctcgcccc tagggcgaga
     1561 cggccaagat gtgcatcacg aatcaacgat tccacaagtt cgacggcccg gtccacggct
     1621 tgctgcacgg gctcgctcaa accgggtgtc acgtcccaat cgagatcgga gcctacgttc
     1681 tcgggctcgc accccaccac gagcacgcgc ttgggaccag ctcccatggt tttcaccatg
     1741 cggaggacct tgaccgggtc gagcgaatgg ggatcgacca aaggctcgtc ggccgcggag
     1801 ttgtcgtgct cgccggcatc gggctcgatg acgtagaggc tgccgggggg acctcgacgc
     1861 ggggccgcgt caacgaggat gacgaggtcg atctcttcga gcagtgccat cgtgagatca
     1921 aagccgcgga tgccgaaatc aacgacactc acgttttggg gccacgcgcg ctctaccagc
     1981 ttccggacga cttcgacgcc gaaggcgtcg tcgcccagga agatgttgcc gatgccggcg
     2041 accaagatgc gcggaagcat ttttagatcc ttggctcgtc gtcgttaagc ggttcgactt
     2101 cctcgggccg gaaaaagaag cggtggccgg gcttgcccgc caggcccatg tcctggccgg
     2161 gatcgtcgtc gacagtgacg gccaggtaaa cgcggtcttc atagtcctgt tcgatggccg
     2221 cgattctggc cattttaccc ttcatggcca tgtcgaggat gtcagcgtct cccaggggcc
     2281 aaagccgtac ccgatcgccg atgtgaaatt cgacgccggc gactcggatg gttgcgagct
     2341 tcggctgttc gatggtcggg tcccagtgat ccattgtttc gatcccgtag gtcgggcttt
     2401 ccagcccgac ataagtaaac gctgtcagac ccagagcctg acttacgtga atggccgtag
     2461 ccctcggaaa gtcccatgga ggccgagcat ttgttcgtta tcgagcaact cggtgcgggc
     2521 caggacggcg cgggcacgct gatccacggc ggccattgcc tgcttctcct gatcggtcaa
     2581 ggtcatgatg cgcagcgtga gaatctcgtc gatctcggtg ccgtcaaaga gctcgccggg
     2641 ggattcgggt gcgatctgcg gataatcgta gagaataatc ggcgcggaga gcattgtgtc
     2701 gttgtccccc tcgtccccga ccaagaccgg ccagcagcca acattgcggc aaagggaggc
     2761 gacgttttta tacggctcgg gcggatcgat cagcgacacg aattcgccat ctcgaacacc
     2821 caaaaccgtg tgggtcgaga ccaggccgtg cgtgagcgct tcgtcgcggt cggcggcgtt
     2881 gttgagatcc aacggggtat cgttctcgat tttcacggtc actttgaata ccccgggcgc
     2941 gacctccgcg gcactcgtct cgacctggcc ttcgatggat ttgcgctcgc gtacgatcgc
     3001 tcccacgatc ttgccgtccg gccatcgcag cggctgtcga tcgcggtgag acgcaagcgc
     3061 gaattcgcgc cgacgggggc agtcggccag cgcaccgagg cttgactccc ccatgtcgat
     3121 cctttgttcg acggcctctt gccaggtgta gaacatttcg tcgccgacct ggagtaattc
     3181 cacggtcggg cagggtatga ctccctcgtc cggccattca tcgaggggat cggcgaactg
     3241 acccaccagc cgcgacacga gctgcagaaa gcgaaccgag atcgtgatcg agctggcggg
     3301 gctgccggct acaaggcatt cggtctgcat ttcaaacggc tcgctcacac actgcaactg
     3361 gctgtacgcc cgcggtacca atccgccgaa cgtccagcgc tggcggttct tcaccgaggg
     3421 gcgatacggg tagaggatgt aaccttcgta gagcacagcg gcggcgatct ggtcgacgag
     3481 tccttggttc atgattcgac ctcctcaagg acagaatcaa tgagcatatc gatcgcctgc
     3541 tcccaggttg gtaagccgcg gctgctcttg tagcggtaga gccgatcgaa cgtgtcgcgc
     3601 tcgagacaga gccaagcgga cttgggataa tagagctcca tcatgttttt ccagacctgt
     3661 accggcagcc ggtacgatgc ttccttttcc cagggaatct ggctgacctg taggtagccg
     3721 tctcgatcgg tgtagaagat cgtgccgctg aagagcaggc agaggggcac ttcaccgtct
     3781 gccagggcgt agaaatactt ggtcgccgcg acgttgaaat cgaacgagca cgggaccggc
     3841 aggtcgatga ccgtttcgcc ggtaaatggc ggtacgacaa cgctggtatg ggtccatagc
     3901 gtgctgcgga gagtgtgccc ccatcgctcg cgcgtgccga acaggtcgag caacttttcc
     3961 tcttcgtcgg cggcgtagcg gcggcgagcc ggctcgatgc ggatctggca ctttagcgcg
     4021 atggccggga tcgtcgtggg ctcttcggcc gcgacgctct cggcgatccg cagcttgaag
     4081 atcaagagcg gcgccgcggc atggggctct acctcggctg tttcgaccgt gaagttcaga
     4141 tccagcacgc gattctcctc atctggtgcg cgaacgctcg tgcaggctct tgaaatatcg
     4201 cccgacttca tcccaaactt ccgtgccgcc ggacaggccg cgccagtgag cccggatcaa
     4261 accgaccagc ttgaaacact cgtcgatcgg tgtgcggtaa taggctcgaa cgtccttcat
     4321 cctgttgacc aggagagcct cgacgtccgg ctcgagctcg cccagcacgg gattgtccac
     4381 gaccagtgtc tgccaggctt cgagagtcag gagcgattcg accgccccgg ccgggctggg
     4441 gtaaagggcg aggacgcgct gggcgggcgt gctctgcacg aagaaagcca ggttgatcgg
     4501 cagatggaga tcttcccact ggatgtcggt caggcggaac tcgggtagcg cccaaatgtc
     4561 gcgcgggact cgtcgatatc ggcccacttg cctgccacta aaaaggacgg cgcacggctc
     4621 gcaactacag acgagctgcc gggtggcggg ctccaggagg tgtgcgtggc cagcggcaat
     4681 tgcaagcccg cagagctcac agcgctcggc cggcggcccg ctgggcttac ggacgaattg
     4741 ccggagggct gacagtgggt tgcgaactgg ggcgtctgca ggttgcatca gttgactcag
     4801 ccgggtttgg gcaaatgttg ttgaatgatc tatgcgctct tatcccgacg tcagtgtcat
     4861 ctccctctcc ccttgtggag gagggttggg gtggggggat gcaggtgcca gacgttcggc
     4921 tgggcctttg cccacggcgg aaacccccca ccctggccct cccccacgag gggggaggga
     4981 attatagatt gctatgtacc agtaagttgt tgctaatgtc cggtattcga ttcacgccgg
     5041 gctccggatc gccatccgcg ggtccagggg cacg
//
LOCUS       DATAQL010000202         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2311802, whole genome shotgun sequence.
ACCESSION   DATAQL010000202 DATAQL010000000
VERSION     DATAQL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311802"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..251)
                     /locus_tag="VHS97_03410"
     tRNA            complement(176..251)
                     /locus_tag="VHS97_03410"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Ala,seq:cgc)
     gene            400..678
                     /locus_tag="VHS97_03415"
     CDS             400..678
                     /locus_tag="VHS97_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX3447272.1"
                     /translation="MYALLNLISTVIQIYIWLLIAQAVLSWLVAFGIVNRHNRVVATA
                     GDFLWRITEPLLRPIRRVLPDLGGIDISPVILILLLYFIRNLMFEYFV"
     gene            671..1000
                     /locus_tag="VHS97_03420"
     CDS             671..1000
                     /locus_tag="VHS97_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX3447273.1"
                     /translation="MSDRPWIMARDGLRVAVRLTPRAKADRLLAIAVAADGKRMVKAS
                     VTAPPEDGRANEALLRLLARVWRLPRRDLTIVAGAASRSKTVCIAGDPLQLSSRLSAL
                     LSTLPGH"
     gene            1014..1724
                     /locus_tag="VHS97_03425"
     CDS             1014..1724
                     /locus_tag="VHS97_03425"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX3447274.1"
                     /translation="MDHALVSLVVAGVAFCGSHTLLSSTRLRGSLRDQLGEQGFLFVY
                     SLTALVTFAWFVVAYARAPTVLVWSPPAWTAWVPVLAMPLATVLLVAGYTTPNPTAVG
                     MERSVRADDPAPGILRITRHPVMWAVGLWALSHLAANGDLASLWFFAAIAVLALGGTV
                     LIDRKKRLALGSHWQRLADATSNIPFAALVAGRTRLRWREIGLLRPVAALLLYAVLYL
                     AHPILAGVPVMIPWVLHG"
BASE COUNT          284 a          636 c          660 g          426 t
ORIGIN      
        1 tcaaccgctg cctgcaatgc gcgcgtgctc gtcaaggcgg gagcggagcg cgacgttcgg
       61 atcgcgcttg ctccgctcag gggaccaggc gggatcgaag gcgccgtggt ggtgatgacc
      121 ccttggcaca cggctgacgc cgaaacctag cgccgcgcga tcgggggtga ccggatggtg
      181 gagccgaggg ggatcgaacc cctgaccttc gcattgcgaa cgcgacgctc tcccagctga
      241 gctacggccc catcgcatgt ttcaagcccc atcggcgggc gcaaccgact atgatgatcg
      301 agccgggggc tgtcaaggct acctcccgct ctgccgcttg cggaccctca ccggcgacgc
      361 tagggtcgac ccactacttg ccgccgaggt ttcggtcaga tgtacgcctt gctgaacctc
      421 atcagcaccg tgatccagat ctacatctgg ttgctgatcg cccaggccgt gctgagctgg
      481 ctggtcgctt ttggtatcgt caaccggcac aaccgcgtgg tcgccacggc cggggatttt
      541 ctctggcgca taaccgagcc gttgttgcgg ccgattcggc gcgtgttgcc cgatctcggc
      601 gggatcgaca tctcgccggt gattctgatc ctgctgctct atttcatccg caacctgatg
      661 ttcgagtact ttgtctgacc gcccctggat catggcgcgc gacggcttgc gggtggcggt
      721 gcgcctgaca ccgagagcca aagcggatcg cctgttagcc atcgcggtcg ccgcggacgg
      781 aaagcggatg gtcaaggcct cggtcaccgc accgcccgag gatgggcgcg ccaacgaggc
      841 attgctgcgg cttttggcgc gggtctggag gcttccgcgc cgcgatctga cgatcgtcgc
      901 cggcgccgcc agcaggtcca agaccgtttg catcgccggc gatccgctgc agctctcgtc
      961 caggcttagc gcgctgctct cgactcttcc gggtcactag ggacgagagg gggatggacc
     1021 atgccctagt gtccttggtg gtcgcgggcg tcgccttttg cggatcgcac actttgcttt
     1081 cgagcacgcg attgcgcgga tctttgcgcg accagctcgg cgagcagggg tttctcttcg
     1141 tatattcact gacggcgctt gtgactttcg cctggttcgt cgtcgcgtat gctcgggcgc
     1201 cgaccgtcct ggtctggtcg ccgccagctt ggacggcgtg ggtcccggta ctggcaatgc
     1261 ctttggcgac ggtgttgctg gtcgccggct atacgacgcc caacccgacc gccgtcggca
     1321 tggaacgatc ggtccgcgcg gatgatccgg ctccaggtat cctgcgaatt acccgccatc
     1381 cggtcatgtg ggcggtcgga ttgtgggcgc tcagccactt ggccgcgaac ggcgatcttg
     1441 cctcgttgtg gtttttcgcg gcgattgcgg tactggcgct tggcgggacg gtcctgattg
     1501 accgcaaaaa gcggcttgct ctcggcagcc attggcagcg cctcgccgat gccacctcga
     1561 acataccatt cgccgcgctc gtcgctggcc gcacccggct gcgctggcgc gagattggcc
     1621 tgttgcggcc ggtcgcggcg ctattgctct acgccgtcct ctatctcgcc catccgattc
     1681 tcgctggcgt gccggtgatg attccctggg ttttgcacgg ctagaatggg aaggttcctg
     1741 ccggtctcgc caccgttatg tttcggcgta gatgctgaca gcgcctggtc atcgaaggcc
     1801 cgccgcgcat tttcggttcc tttgttcggt cctcacccca accttccccg cgttgtggga
     1861 aaggggataa ccgcattgtc ggccccattg atggggagag gtcagcacga gaaccggcgt
     1921 cgggcggtga ggcggaggcg gagggcgttc agtttgatga agccttcggc gtcgcgctgg
     1981 tcgtagaccg cgtcttcctc gaaggt
//
LOCUS       DATAQL010000203         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1548390, whole genome shotgun sequence.
ACCESSION   DATAQL010000203 DATAQL010000000
VERSION     DATAQL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548390"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VHS97_03430"
     CDS             <1..407
                     /locus_tag="VHS97_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447275.1"
                     /translation="KIEAGKKASLVLDAEANSPPIPFPNGVLLDDADHLLIADFSQGY
                     IFRLDLTTKKLERIGSGFGGTDGLAKDARGRRFVGDWKNGKLFELVSENEPPRLLSDM
                     FQSAADIALMPDGKTLLVPDMKAGTLTWFPVR"
     gene            449..1363
                     /locus_tag="VHS97_03435"
     CDS             449..1363
                     /locus_tag="VHS97_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3447276.1"
                     /translation="MASNPQNLLSVREILVLGPYGVVGTGVVDAATANPAWRVVTAAR
                     RPVPSHRDSTAVRHVNVDLLDREGTRAVLSKLDTVTDLVFAAYVEKSTMAETVEPNAR
                     MLQNTLDAFAARDVPLRRVVLAGGAKSYGFHLGSFNAPAKESQPRLIAPIHYHQQEDI
                     LKAWWEKTGTVWTVLRPHVVLGPSLNSPMNLVTGVATYAAMSRELGLPLRFPGRKEGW
                     NSLQEATDAELFGRAALWALGSDTAKNEIFNVSNGDVYRWRQLVASTLGLPPRLKLIR
                     SQPSATARSINLAIAKFVAWSLPLPKTR"
     gene            complement(1560..>3305)
                     /locus_tag="VHS97_03440"
     CDS             complement(1560..>3305)
                     /locus_tag="VHS97_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3447277.1"
                     /translation="RDSIETLTSLAAVPPIRRFALHALSDHRADAGRVPDRPFVEGLT
                     DADPRVRLQAVIGLGRLGKGAGASAIVERTADDDPLVAHVAVQALVALNAVSACLDAL
                     DPATPRLAPGAARALQAMHTAQAVDGLIAKLEKSSDAATHRLVFNALCRLDHREADYT
                     GDWWTTRPDTSGPYYKPVTWAETPKIERALEGAFERADKRSVSSLLVELVRNKVELKA
                     TAAVDFDFAGLEPSNRAVMVDILGGRRSLPDKAIRFLDGIAASDQEAPALRVRALKGL
                     VRQDRRSAAGIMAVIGHQEKPAPELLDAWREYLRDGGHPRRLGDWRTMAQDKDPARRE
                     LAYAVLLAVDANPRTFRQARADAERAIESAGKSPSSTASLLRAIGQTDSVKYAFQVKN
                     GLKSSDPEVKSAAVFAAQRLDLDGEGDGPGRGPTIASLPFETVVAAATSENGDAPRGE
                     RLFLRQGCAACHTVASGAPPKGPSLAGIAQRYNRAALAESILKPSAKLAQGFEPQKFA
                     TVDGRTFDGFVVRESGDEVECRDAQGSVFILPKKEIDARAKGDVSIMPTGLIDALTVH
                     DLASLLTYLESLKSK"
BASE COUNT          579 a         1098 c         1042 g          586 t
ORIGIN      
        1 tcaagatcga agcgggtaag aaggcgtccc tggtactgga cgccgaggca aactcgcccc
       61 ctatcccgtt cccgaatggc gtgctactcg acgacgccga ccacctgctt atcgccgact
      121 tcagccaggg atacatcttc cggctggact tgacgaccaa gaagctcgag cggatcggca
      181 gcgggttcgg cggcaccgac ggcctcgcca aggacgcgag aggacgccga ttcgtcggcg
      241 actggaagaa cggcaagctg ttcgagctgg tctccgagaa cgagccgccc aggctcctct
      301 ccgacatgtt ccagtcggcg gcggacatcg cgctcatgcc ggacggcaag accctgcttg
      361 tgccggacat gaaggccggg acgctgacct ggttcccggt ccgctgaatc gtcgcccaga
      421 ccactcgccg caacgagagg agacgatcat ggcttccaat ccccagaacc tgctctccgt
      481 ccgcgaaatc ctggtgctgg gcccctatgg cgtggtcggc acgggcgtcg tcgatgccgc
      541 cactgccaac cccgcctggc gcgtcgtcac ggccgcccgc cgtcccgttc cctcgcatcg
      601 cgattcgaca gcggtgcggc acgtcaatgt cgatctgctc gaccgcgagg gcacaagggc
      661 ggtgctctcc aagctggaca ctgtgacaga cctcgtcttt gcggcctacg tcgagaagtc
      721 gacgatggcc gagacggtgg aacccaacgc aaggatgctt cagaacacgc tcgacgcgtt
      781 cgccgcccgc gacgtcccgc tccggcgcgt cgtcctcgcg ggcggggcca agtcctacgg
      841 tttccacctc ggctcgttca acgcgcctgc gaaggagtcg caaccccgcc tcatcgcccc
      901 gatccactac caccagcagg aggacatcct gaaggcgtgg tgggagaaga ccgggacggt
      961 ctggacggtc ctgcgcccgc acgtggtatt gggcccgagc ctgaactcac cgatgaacct
     1021 cgtgacgggc gttgccacgt acgccgcgat gagccgggaa ctcggcctgc cactgaggtt
     1081 tcccggccgc aaggaagggt ggaactcgct ccaggaggcc accgacgcgg agttgttcgg
     1141 gcgagccgcg ctttgggcgc tcggatcgga cacggcgaag aacgagatct tcaacgtctc
     1201 gaacggcgac gtgtatcgct ggcgccagct ggtggcctcg accttggggc tgccacccag
     1261 actcaaattg atcaggtcgc agccatcggc gaccgcacga tcgatcaact tggcgattgc
     1321 gaaattcgtc gcctggtcac tccccttgcc aaaaacgcga taactccgta gcgtgacccc
     1381 cggcgccatc ccgcgtatcc ccttgccgga cgcaccgtgt gcgccgatga ttcccgcgac
     1441 atgggtgccg tggccgcctc cattatcgcc gaagtcgccg gtcttctcgc cggtcaccgt
     1501 gttctcaccc cctcgaatgc tcagatcagg gtgtttcaag tcggtgatac ccagatcgct
     1561 tatttggact tgagagattc gaggtaggtc agcaacgagg cgagatcgtg aactgtcaga
     1621 gcatcgatca agccggtagg catgatggac acatctccct tggcacgagc atcgatctct
     1681 tttttcggca aaatgaaaac gctcccctgg gcgtctcggc actccacctc atcgcccgat
     1741 tcgcgtacca caaagccatc aaacgtccgg ccgtccacag tggcgaattt ttgcggctcg
     1801 aagccctggg ccaatttggc gcttggtttt aagatcgact cagcgagcgc ggctctgttg
     1861 tagcgttggg cgatgcccgc gagcgatggc ccttttggag gcgcgccgga cgcgacggtg
     1921 tggcaggcgg cgcacccctg tcgcagaaac agccgctcgc cgcggggcgc atctccgttt
     1981 tcggatgtag ccgcggcaac aactgtctcg aagggtagcg atgcgatcgt cggaccccgg
     2041 cccggcccgt cgccttcacc gtccaagtcc aatcgctggg cggcaaagac cgctgcactc
     2101 ttcacctctg ggtcggagga cttcaggcca ttcttgacct gaaaagcgta tttcaccgag
     2161 tcggtctgtc cgatcgcacg cagcaagctg gccgtgcttg atggcgactt gcccgccgac
     2221 tcgatcgccc gctcggcgtc ggcccgcgcc tgtcgaaaag ttctcgggtt agcgtccacg
     2281 gcgagcagca cggcataagc cagctcgcgc cgcgccgggt ctttatcttg ggccatggtt
     2341 cgccaatctc cgagacggcg gggatgcccg ccgtctcgga gatattcccg ccatgcgtcc
     2401 agcagttcgg gcgctggctt ctcctggtgc ccgatcaccg ccatgatgcc ggcggccgag
     2461 cggcgatctt ggcggaccaa ccccttcaaa gcccgaacgc ggagcgccgg agcttcctgg
     2521 tcggaggcgg ctataccatc caggaaacgg atcgccttgt cgggaaggga acggcggccg
     2581 ccgaggatat cgaccatgac tgcgcggttc gacggctcga gtcccgcaaa gtcaaagtcg
     2641 accgctgccg ttgccttgag ctcgaccttg ttgcggacca gctcgaccaa gagtgaactg
     2701 actgaacgct tgtcggcgcg ctcgaaggca ccctcgaggg cccgctcgat cttgggggtc
     2761 tctgcccagg tcacaggctt gtaatatggc ccgctggtat ccggtcgggt ggtccaccag
     2821 tcgccggtat agtcggcctc gcggtgatcg agacggcaca gagcgttgaa gacgagccga
     2881 tgggtggcgg cgtcggatga tttttccagc ttggcgatca ggccgtcgac ggcctgggca
     2941 gtatgcatcg cctggagggc gcgggcagcc ccaggcgcga gccgcggggt cgcgggatcg
     3001 agtgcgtcaa ggcacgcgct gacggcgttc aatgccacca gcgcctgcac ggcgacgtgt
     3061 gcaaccagtg ggtcgtcgtc agccgtccgc tcgacgatcg ccgaggcacc ggcgcctttg
     3121 cccagccggc ccagcccgat gaccgcttgg agccgcaccc gtggatcggc gtcggtcaac
     3181 ccctcgacaa agggcctgtc cggcacgcgc ccggcgtctg cccgatggtc gctcaaggca
     3241 tgcaatgcaa accgccggat tggtggcacg gctgccaggc tcgtcagagt ttcgatcgaa
     3301 tcacg
//
LOCUS       DATAQL010000204        13753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2312004, whole genome shotgun sequence.
ACCESSION   DATAQL010000204 DATAQL010000000
VERSION     DATAQL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312004"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1242
                     /locus_tag="VHS97_03445"
     CDS             31..1242
                     /locus_tag="VHS97_03445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX3447278.1"
                     /translation="MKTTDAVWITGVGAATPLGCTLEAIEANLLAGRSGVSLVTRFST
                     ADYPSKIAAQLSTPLHPFDGDVLSFSARPPLDQLAHWCVESALRDAGLWGSHREARVG
                     LVLGIGAEWMLHWEADSSRGGTRLYDPELDRDSTIERVHRALGLAGPALALSAACASG
                     NHALEIGRHWLRLGLVDICVAGACDLAVSPIGLATFGNLRALSRRNDDPAAASRPFDR
                     GRDGFVLGEGGVAFILESATRARRRGANAYAEVAGCGSSSDAFHHVIPSPDPAQAAAA
                     MRQALADAQVEPGDVDHVNAHATSTPVGDAAEAAVLRLVLGDDAGRIPVTSTKSLSGH
                     MLTAAGAFEALACLTAMRHQAIPPTINLDDPDVDLCLVANEARPHPVRVVVSNSFGFG
                     GSNTSLALRAV"
     gene            1360..2145
                     /locus_tag="VHS97_03450"
     CDS             1360..2145
                     /locus_tag="VHS97_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3447279.1"
                     /translation="MAKQGAVVIVTGASSGIGRALAMRLGLLGYRVGLIARRREALES
                     AAAGISRSGGQAVSAVADVSDRSQLRSAVAEIERSLGPADVMVANAGFGVPTQLDPLN
                     TCEVEQTIRVNVLGVIYSIEAVLPGMIARKCGHVVGISSLGAFKGMPGESAYCASKAA
                     VNAYMEGLRIALRSKGVVVTTVCPGFVDTPMTPMDSARPFLMSADGAACRIARLIATR
                     RGGVVRFPLSMSLLMSLFARLPDALVARLMRIESVPERAAAAE"
     gene            2151..3278
                     /locus_tag="VHS97_03455"
     CDS             2151..3278
                     /locus_tag="VHS97_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3447280.1"
                     /translation="MIDLALKMLLDEKVRFAATVLGVGFAAALIMIQMGLFFGLLENA
                     SITIERLDADLWVTARNTANVDFGNPFPETYVQRVRSVPGVERADNLIVWYAIVALPT
                     GAKESVIYYGLKDFPAWNFPWNVESGDPADLRRGQFVMVDASAERRFGPFRTGDHREF
                     QGRRLKIIGRTREALSFTTSPVAFLDYELAQSLSPNELVGRTTFVIVRLEPGADASAV
                     TREIRRRLPYNDVYGQAEWAARSRGYWIESTGLGLTLFLTVALGAMVGVVIVAQTLYA
                     STCEHLVEFGTVKALGGGNRTVYAIVAEQALFAAALGFGLALALAYGLAPILSRLDMK
                     MIVTPTLAGLVFVGTQALCVAAASLSFHKVASLDPAMVFRG"
     gene            3305..3985
                     /locus_tag="VHS97_03460"
     CDS             3305..3985
                     /locus_tag="VHS97_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3447281.1"
                     /translation="MAVLEGRDLTKTYRQGKLEVPVLHGVSLAVERGEVVALEGPSGS
                     GKTTLLCILGCLLTPTGGRIVIDGHLIDENRPDRLQEIRRRSIGFVFQQFNLFPSLTA
                     SENVEYALNLKGWRGRTARHEAERTLESVGLGDRKNFRPRDLSGGQRQRIAVARAMAG
                     AAPLILADEPTGSLDSESGNIVLGLFRDLAKSGSRALLIVTHDPLVRAIADRVLSIRD
                     GRLTPGET"
     gene            3990..4898
                     /locus_tag="VHS97_03465"
     CDS             3990..4898
                     /locus_tag="VHS97_03465"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447282.1"
                     /translation="MSPTMFLGRVVLPAIGLVVAVGVTWQSFRKVTARTEVISVGETA
                     RSAARSTGRIIAEGRVVAYPGAQATVGTEVMGTIINMPARETVAVRKGDLLVEFRSDD
                     IKASLGEAHFRLIEAEAGIRLLRARYQLDRIFPALSAKTPQPADNRLDEYAAAQARRD
                     AAKAAVDRLEAESAKYRIMAPINGVVIARHAQPGETVTPSSSLATIADLSRLRIEAEV
                     DEFDIAGVSLGAKATITVEGYPGQVWQSKIEEIADAVVPRETRPEDPGRSADTRVLKV
                     KLALLEPNPFKLGQHVEVEILRRAAE"
     gene            complement(4988..5060)
                     /locus_tag="VHS97_03470"
     tRNA            complement(4988..5060)
                     /locus_tag="VHS97_03470"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5025..5027),aa:His,seq:gtg)
     gene            5391..6422
                     /locus_tag="VHS97_03475"
     CDS             5391..6422
                     /locus_tag="VHS97_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447283.1"
                     /translation="MRAGKSTMGSSELRIGIAGCGTAARIHLDRLLTLEGVAIVGCAD
                     TDLAAAKALVDRATQGPLARGEETVPAFGDHRELIRALAPDALAIFTPHLSHYRLTMD
                     ALQAGCHVFIEKPLSTNVQEAADIVTLARGRDLKVGVGHQYRLCPSLIEARQRIASGA
                     IGPVRLVSCILARPWLTTLGPQEREWRASGQFAPGGILSDAGDHLIDALLWTTGQFAR
                     EVGAIQSHGDSGIDLVTAAAIRLADGAPATLAISGTSPGNVFVLDYFGERGRLRATDQ
                     TLEQELFDSLRQDVVLPPANQTIDGNFVAALTKETPLCCSADQALDTVRLLEAIGRSA
                     ATGHVVRLI"
     gene            6779..7210
                     /locus_tag="VHS97_03480"
     CDS             6779..7210
                     /locus_tag="VHS97_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447284.1"
                     /translation="MAIKSALVRGNSPMNSLGLRTEVMLDCLEPLTTLADPTAAKANW
                     DGWSDLPPPAVGRHPGDEEEEVPEEEIDGEETLDGDDFEDDEEEFDDEEFDDEDEEDI
                     DEELDDEIDDIDVDDDEEDDFDDDEDLDELDEEEDDELEDI"
     gene            7622..8062
                     /locus_tag="VHS97_03485"
     CDS             7622..8062
                     /locus_tag="VHS97_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447285.1"
                     /translation="MAAPLNRPNDSSSERPSPETSTRRLREQSQIEFEIDFLGRVLER
                     DPFYVDALRVHGNNLAAKGLYGRALQIDRRLVRLISDDAIAWYNLACSYAVLGMIEPA
                     FSALQRSLELGYHFLTRLRRDPDLKALRRDPRFADLLRRFEFLV"
     gene            8063..8893
                     /locus_tag="VHS97_03490"
     CDS             8063..8893
                     /locus_tag="VHS97_03490"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX3447286.1"
                     /translation="MTHHEWEHVQAASQMVGSRWPGKPTVGLVLGTGLGALAREIEAE
                     ATIPYPEIPYFPRSTVESHKGQLVCGTLAGKSVLAMEGRFHLYEGYSPPQVTFPIRVM
                     KELGCRLLIVSNAAGGLNPLTSKGDLIVIEDHINLMGLNPLIGPNDDRLGPRFPDLIE
                     PYDRELQSLALKIALEKNIVAHKGVYVAVVGPNLETRAEYRFLRSIGADVVGMSTVPE
                     VLVAVHAGMKVLGFSIITDMCLPDALQPVRIEEIIAVANLAEAKLRTVVTGVLERWNG
                     "
     gene            9031..11844
                     /gene="ileS"
                     /locus_tag="VHS97_03495"
     CDS             9031..11844
                     /gene="ileS"
                     /locus_tag="VHS97_03495"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX3447287.1"
                     /translation="MSTKSKPYKETLNLPVTRFDMKANLPVREPQIQARWLEQRLYEQ
                     VRQAKADQPRKVLHDGPPYANGEIHMGTLLNKVLKDFVVRSLTMAGFDSPYVPGWDCH
                     GLPIEHKVVKELGSKAAALNHAEIRALCQTEAMKWVDVQRTQFRRLGVMGDWDNPYLT
                     LDPRYEAGILDILAELVEGGYVGRQKKPIHWCMTDRTALAEAELEYREETTPSIYVNF
                     PLVSGVPQGWSGSGTWHAMIWTTTPWTLPANVAIAVHPELEYVGVRYVDPATGHAVQT
                     IIAAELVAKVMGLRQVADFAEVGRCRGRELEHSEYRHPFVDRVSPIVLANYVSVTDGT
                     GLVHTAPGHGAEDYQTGRVYRLSILSPVDESGRFTSDAPLWLTGQHVFAANRVIIDRL
                     KAGAHLYHEQQLEHSYPHCWRCKKPVIFRATEQWFISVDHDDLRGRTLKSIDEVCWLP
                     EWGQTRIEAMVSLRPDWCISRQRSWGVPIPALGCTACHAQLLTAETVRHFRDLFRTEG
                     ADVWFTRPVEQLVPPGAVCAHCGGTSFRKEGDILDVWFESGSSHRSVLAKEFNLGFPT
                     FMYLEGSDQHRGWFQSSILTSVGTTGRAPFETVLTHGFVVDDKGRKISKSEGNYIPAE
                     KMTSQYGADVLRLYVASMDYADDISVSERGIKEMSEAYRKIRNTFRYLLGNLDDYRHF
                     NPETVDLTWLHPIDRWALRQLNKVVRDVRAAYERFEFYRVHQRIYQFCAVELSSFYLD
                     VLKDRLYAEAPAGPDRRAAQFVLARLHDHLARLLAPIIPHTAEESWDYLPATSAKASS
                     VHLAEFPEPDRAWDDDADDARWDGLLKLREQILIALEGLRKSKQIGSAQEAKVSVTTN
                     RPEHWLPDGELLATLCNVSEVEIMGDSTATIEAVTAEHASYAKCERCWNYRPTVGQSP
                     EHPTLCERCVRVLSELDGGRATE"
     gene            11921..12511
                     /locus_tag="VHS97_03500"
     CDS             11921..12511
                     /locus_tag="VHS97_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3447288.1"
                     /translation="MATTTLMDEIDIANEIEVDRLDDELYEVVDGKRIRRLPMSAYSA
                     TIASRLVRKLGMFTDERGLGETVGEVLFRLPLLADLFRNRRPDVAYVSFGRWPSGRAM
                     PLKDNAWDVVPNLAVEVISTHDLAEESLQKIGEYFEAGVQLVWVVYPQQRLVYVYQTP
                     TRVHILATDDTLDGGAVLPGFQMRVCSLFDTIAPAN"
     gene            complement(12580..>13753)
                     /locus_tag="VHS97_03505"
     CDS             complement(12580..>13753)
                     /locus_tag="VHS97_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447289.1"
                     /translation="YGWFLRWLMDKGDGSPFPEPPTEMQRFDDLELRCFPPGQNQPAG
                     PAIVAMVERLAHDLPVNSPDRRLSLESVLGPWPALEREQTGLRVEPVQLQRLMIPTEP
                     GINVPAFLLNAPGNARGIIVAVADNGKESIGSDPVIAAAIESRWAVCGVDPRGIGESS
                     SAKTGWIFAVSLLLGENFVGRQAFDLQQVIDNLGTTDAFAGKPIGLYARGPNASLMAV
                     YAMAHRQLTALARSREGAPPGEPRRKPARTEPYPSGITQGPSNLHRQSQLNWFLLRDV
                     FLSYRAFFERPRALSESFRLMPADGNRTTSFDREIPASFFVVDVLHSWDLPQLLSSIS
                     AQGLIVNAIDGEGDRLSERAALKLLPRHIRVVSGAEPHQGIKNFLRSVLVERGTSP"
BASE COUNT         2617 a         4109 c         4273 g         2754 t
ORIGIN      
        1 gacgggtcgg acacaccagg atgaggcgac atgaaaacga ctgatgctgt ctggatcacc
       61 ggtgtcgggg cggcaacccc gcttggctgc acccttgagg cgatcgaagc aaacctcctg
      121 gccggccggt cgggtgtctc gcttgtgacc cggttttcga cggccgacta tcccagcaag
      181 atcgcagcgc aacttagcac gcctctacac ccattcgacg gcgacgtcct ctctttctcg
      241 gcacggcccc cgctcgatca gctcgcccat tggtgcgtcg agtcggccct gcgcgacgcg
      301 ggtctgtggg ggagccatcg cgaggcgagg gtcggattgg tgctggggat cggtgccgag
      361 tggatgctgc attgggaggc cgacagtagc cgtggcggga cgcggcttta cgacccggag
      421 ctcgaccgcg attcgacgat cgagcgcgtc catcgtgcac tgggtctcgc gggccccgcg
      481 ctggcgctgt cggcggcctg cgccagtggc aaccacgctc tcgagatcgg ccgccattgg
      541 cttcgcctcg gtctggtgga tatctgcgtc gcgggagcct gcgacctggc ggtgtcaccg
      601 atcggccttg caacctttgg caatctgcgc gcgctgtcac gccgcaacga cgatccggcc
      661 gccgcgtcac gtccgtttga tcgcggtcgt gacggattcg tgctgggtga gggaggagtc
      721 gcgttcatac tcgagtcggc cacacgagct cggcgccgcg gcgcgaatgc ctatgccgag
      781 gtcgccggct gcgggtccag cagcgacgct tttcaccatg tgattccgag cccagacccc
      841 gcgcaggccg ccgctgccat gaggcaagca ctggccgatg cgcaagtcga gcctggcgac
      901 gttgaccatg tgaacgctca cgcgacgagc acaccggtcg gcgacgctgc cgaggccgcc
      961 gtcttgcgcc tcgtcctggg agatgacgcc ggccggatcc cggtcacctc gaccaagagc
     1021 ttgagcggcc acatgctcac cgccgccggc gcgttcgagg cgctggcttg tctgacagcc
     1081 atgcggcatc aggcgatacc gccgacgatc aatctcgacg atcccgatgt tgacctttgc
     1141 ttggttgcca acgaggcccg tccgcatccc gtccgcgtcg tggtgtccaa ctcgttcggc
     1201 ttcggcggca gcaatacgag cctggcgctc cgggcggtgt aggagatccg ctctgccgcg
     1261 aaaagccttg aatctggaat aaggcctcgt tgatccttga aattagggaa tgaccgatat
     1321 tctcagttca cgctggcacc ggaggcgaat gagttggatg tggcgaaaca aggtgcggtg
     1381 gtgattgtca ccggtgcttc gagtggcatc gggcgggccc tcgctatgcg gctgggttta
     1441 ctcggctatc gcgtgggcct gatcgctcgc cggcgcgaag cactcgaatc ggcggccgcc
     1501 gggatcagcc ggtccggcgg acaggccgtt tcggccgttg ccgatgtttc ggatcgctcg
     1561 caactgcgct cggccgtcgc cgaaatcgaa aggagtcttg gaccggccga cgtgatggtt
     1621 gccaacgcgg gctttggcgt accaacccag ctcgatcccc tgaacacgtg tgaagtcgag
     1681 cagacgatcc gggtcaacgt cctgggcgtc atttactcga tcgaggccgt gttaccgggt
     1741 atgattgcac gcaagtgcgg gcacgtggtg ggaatttcga gcttgggtgc gttcaagggc
     1801 atgcccggcg agtcagccta ttgcgcgagc aaggcggccg tgaacgcgta catggaggga
     1861 ctgcgaatcg ccctgcggtc gaaaggggtt gtggtgacaa ccgtgtgccc cggctttgtc
     1921 gacacgccga tgaccccgat ggattctgcc aggccgttcc ttatgtcggc cgacggtgcc
     1981 gcctgtcgca ttgctcgttt gatcgcgaca cgacgaggag gcgtggttcg ctttccgctc
     2041 tccatgtcgc tcttgatgtc tctctttgct cggttgcccg acgcactggt cgcgcggcta
     2101 atgcgaatcg agtccgtccc cgagcgagcc gcggcggcgg agtgagcacg atgattgacc
     2161 ttgccctcaa gatgttgctc gacgaaaagg tccgctttgc ggccaccgtg ctgggagtcg
     2221 ggttcgccgc ggccctgatc atgatccaga tgggtctatt ttttggcctg ctcgagaacg
     2281 cgagcatcac gatcgaacgg ctcgatgccg atctttgggt gacagcgcgg aacacggcca
     2341 acgtggactt tggcaaccca tttcctgaga cctacgtaca gcgggtgcgc tcggttccgg
     2401 gcgtggagcg agcagataac ttgattgtct ggtacgcaat cgtcgccttg cccaccggtg
     2461 ccaaggaatc ggtcatttat tatggattga aggattttcc ggcctggaac ttcccatgga
     2521 acgtcgagtc gggcgatcca gccgatctcc ggcgtggcca gtttgtgatg gtcgatgcgt
     2581 ccgcggagcg ccggttcggg ccgtttcgca ctggcgatca ccgtgagttt cagggacggc
     2641 gtctcaagat tatcggccgg acccgcgaag cgctctcgtt cacgaccagc cccgtcgcct
     2701 tcctcgacta tgagctcgcc caatccctct cgcccaacga gctagttggt cggacgacat
     2761 tcgtcatcgt tcgactcgag ccaggcgctg acgccagtgc ggtcacacgc gagatccgcc
     2821 gccgcttgcc ttacaacgac gtttacggcc aggctgaatg ggcagcccgg tcgcgcggct
     2881 actggatcga gagtaccggt ttggggttga ccttgtttct caccgtggcg ctcggcgcga
     2941 tggtcggcgt tgtcatcgtg gcgcaaaccc tctacgcttc gacgtgcgag catctcgtcg
     3001 aatttggaac tgtcaaagcc ctgggtggcg gcaatcgcac tgtttatgcg atcgttgcgg
     3061 agcaagcgtt gttcgcggct gctctgggct ttggactggc actggcgctg gcatacggac
     3121 tggctccgat tttatcaaga ctcgacatga agatgatcgt cacgccgacg ctggcaggtc
     3181 tggtcttcgt gggtacgcag gcgctctgcg tcgctgccgc gtcattgtcg tttcacaagg
     3241 tcgcgtcgct cgacccggca atggtgtttc ggggttgagt ggtgatggtg gaaggggctt
     3301 tcgcgtggcg gtactcgaag gtcgcgattt gacgaaaacc taccggcagg ggaaacttga
     3361 ggttcccgtg ctccacggtg tctcactggc ggtcgagcgg ggcgaggtgg ttgcgctcga
     3421 agggccgtcg ggttcgggca agacgacgct cctatgcatc ctgggctgcc tgctgacccc
     3481 gaccggaggt cgcattgtca tcgacggaca tttgatcgat gagaatcgtc cggatcgttt
     3541 gcaagaaatc cgccgccgct cgattggttt cgtcttccag caattcaacc tctttccctc
     3601 cctgacggcc tcggagaatg tcgagtacgc ccttaatctc aagggctggc gggggaggac
     3661 tgcccgccat gaggccgagc ggaccctcga gtctgttggt ttgggggatc gcaagaactt
     3721 tcgtccccgt gacctctcgg gcggccagcg gcagcgcatc gccgtggcgc gggctatggc
     3781 cggagccgcg cccttgatcc tcgccgacga accgaccggc agcctcgact cggagagcgg
     3841 taatatcgtc ttgggcttgt tccgcgatct ggcgaaatct gggagtcgag cgctcttgat
     3901 cgtgactcac gacccgctcg tccgcgccat cgccgatcgc gttttgtcga tccgcgacgg
     3961 tcgcctgact ccgggagaga cttgaaacaa tgtcaccgac gatgttcttg ggacgggttg
     4021 tcctgccggc catcggcctc gtcgtggcgg tcggcgttac ttggcagtcc tttcgcaagg
     4081 tgactgcccg taccgaggtc atcagtgtgg gagagaccgc gagatccgcc gcccgctcga
     4141 ccggcaggat cattgctgag ggacgagttg tcgcgtaccc cggtgcccag gcgactgtcg
     4201 gcacggaggt catgggaacc atcatcaaca tgcccgcgcg tgaaaccgtg gccgttcgca
     4261 aaggcgattt gctcgttgag ttccggtccg acgacatcaa agcttcgctg ggtgaggcac
     4321 attttcgact gatcgaggct gaggcgggca ttcgcctttt gcgtgcgcgc taccagttgg
     4381 accgaatatt ccctgccctg agtgcgaaaa cgccgcagcc tgcggacaat cggctcgacg
     4441 aatacgcggc cgcccaggct cgtcgcgacg ccgccaaggc cgccgtcgac cgtctggagg
     4501 ccgagtcggc caagtaccga atcatggcgc caatcaatgg tgttgtcatc gcacgccatg
     4561 cccaacccgg cgagacggtc accccaagtt cgtccctcgc cacgatcgcg gacttgtcca
     4621 ggctgagaat cgaggccgaa gtcgatgaat tcgacattgc cggagttagc ctcggggcga
     4681 aggcgaccat caccgtggag ggttatcccg gccaggtctg gcaaagcaag atcgaggaga
     4741 tcgccgatgc tgtcgtcccc cgtgagactc gccccgagga tcccggcagg tccgccgaca
     4801 cacgcgtact gaaggtcaag ctggctttgc tcgaaccgaa tcccttcaaa ctggggcaac
     4861 acgtcgaggt cgaaattcta cgccgcgccg ccgagtaatg atcgcaagcc aatctcgccg
     4921 ttcttgagat tgcgaccaga ctgtaagcat gtgtgtggga gatgtcgtgc cagaattgca
     4981 ctaaaagggt gggtgacggg attcgaaccc gcgatatcca gatccacaat ctggtgcctt
     5041 aaccgctagg ccacacccac catcattcat atcgaaccac tctgacacga tataccctgg
     5101 tttgggttca cgtcaaggcg atcagcgagg gtcgcacgat ggcatgtagg cgcgtcattt
     5161 gggcgcgatt gcgcgagagt tggcgaccga tgagaggtgg cggcggaccg cccgattcgt
     5221 gtgtccgcca ccccaaagca aagaaatgaa aataaggtct tgacatcgct gtataaactc
     5281 ttgacgaaag gcggacatat tgctgggatg aagccgcgat tttgctggag cctcggcaac
     5341 gccgaaatca atgcggctca tctggtttga gtttacgctc aagaaacaaa atgcgggcag
     5401 gcaagagcac catgggatcg tcggaactaa ggatcggaat cgccggctgc ggcacggcag
     5461 cgcgaatcca cctggaccgg cttctgaccc tggagggggt ggcgatcgtc ggctgcgcgg
     5521 ataccgacct cgcggcggcg aaggcactgg ttgaccgagc aacgcaaggg cctctggccc
     5581 gcggtgagga aaccgtccct gcgttcggtg atcatcgtga gctgatccgc gctctggcac
     5641 ccgacgcgct ggcgattttc accccccatc tgtcccacta ccgactgacc atggatgccc
     5701 tccaggcggg ctgccatgtg ttcatcgaga agcctctctc caccaacgta caagaggccg
     5761 cggatatcgt caccttggct cgcgggcgcg atctcaaggt gggcgtcggg catcaatatc
     5821 gcttgtgtcc tagcctcata gaggcgcgcc agcgaatcgc ctcgggcgca atcggcccgg
     5881 ttcgattggt gagctgtatt ctcgcccgtc cctggttgac gaccttgggc ccacaagagc
     5941 gcgagtggcg cgcgagcggc cagtttgccc cgggcggtat cctatctgat gccggcgacc
     6001 atctcatcga tgctctactc tggacaacag gacagttcgc gcgtgaggtg ggggcgattc
     6061 aaagccatgg agactcggga atcgatctcg tgacagccgc ggccatccgg ctggctgacg
     6121 gagcgccggc cacactggcg atatccggca cgtcgccggg aaacgttttc gtgctcgatt
     6181 actttggcga gcgcggccga ctgcgggcca ccgaccagac tctggaacaa gagcttttcg
     6241 actcactgcg gcaagacgtt gtcctgccgc ccgccaacca aacgattgac ggcaactttg
     6301 ttgcggctct gaccaaggaa acgcccctat gctgttcagc cgatcaagcg cttgacactg
     6361 tgcgactgct tgaagccatc gggcggtcgg ctgcgacagg ccacgtagtt cgtctcatct
     6421 agtgcaatcc atgtatcgtg aaaggagcgg gctgggcgac ctgaaattcc gatcacttgt
     6481 tcgagccgtc ggtccgggaa tcttgccttc gaaatgcatg gagcgatcac cgattttgcg
     6541 gcggattcgc ctgcacaaag agtctttcaa ggttccccgc ctgccatgcc gaccattgcg
     6601 agtagtaaat gatcgtcatg cctcaagact gcatgacttc gatacgacgg gcacagccac
     6661 ggaatgcgtg ctctaaccca atggaatctc gttatttcga tacttgacag taatgcctct
     6721 ccttgactac gactatggct ccgctctcgg atcgaacagg gttcacccaa gaagcgaatt
     6781 ggccatcaag agcgcgttgg ttagggggaa ttccccgatg aactcccttg gcctgaggac
     6841 tgaagttatg ttggactgtc tcgaaccgct caccacgctc gccgacccaa ctgccgcgaa
     6901 ggcgaactgg gatggctggt cagacctacc cccgccggcg gtgggtcgac accccggtga
     6961 cgaagaagaa gaagtgcccg aagaagagat cgacggcgag gaaacgctcg acggcgatga
     7021 cttcgaggac gacgaggagg aattcgacga cgaggaattc gacgatgagg atgaagaaga
     7081 catcgacgaa gaactcgacg acgagatcga cgatatcgac gtcgatgacg atgaggaaga
     7141 cgatttcgac gacgacgaag acttggacga gctcgatgag gaagaagacg acgagctcga
     7201 agatatctga tcgtcgaaat gtgtggtcaa gctggccgaa taagtgtcgc cacctctggg
     7261 ttgtctccgg ttcggcaatg atggatgaat gagtgcacgg ggtgaattcc gcgctcaacc
     7321 gtggcgttga ccccgacttt catgtgcctg gactcttgtg gagacgggtt ccttacggct
     7381 attctggcac aagattggtc gagtcgccgg aaaactaaat tcttgttgcc agggcaggcc
     7441 cgatcgccat accatagtac gctggctttt tcaaactcca ggcggactgc aagtgtgcgt
     7501 cggcttcccg ttttgggatc gtgtttcagg tgctgattcc gggtcgcgat cgccttccat
     7561 cactgcgccc tacacgttgt gagctcaggc tttgcaccac tgatcgaggg agcacgttgc
     7621 gatggccgca ccgctgaatc gtccgaacga ttcgtcttct gaacgcccgt ctcccgagac
     7681 gtcgacacgg cgattgcgcg agcaaagcca gatcgagttc gaaatcgatt tccttggccg
     7741 ggttctggag cgagatccgt tttacgttga cgcgttgcgg gttcacggga acaacctggc
     7801 agcgaagggg ctttacgggc gtgcgctcca gatcgatcgc cggttggtca ggctgatctc
     7861 cgatgacgcc attgcgtggt acaacctcgc gtgcagctac gccgtactgg ggatgatcga
     7921 gcccgccttc tctgctcttc agagatcgct ggagttgggc tatcacttcc tcacgcggct
     7981 acgccgtgac cccgatctca aggctctgcg ccgtgaccct cggtttgctg atcttctacg
     8041 gcgtttcgag ttcctcgtct aaatgacgca tcacgaatgg gagcacgtcc aagcggccag
     8101 ccaaatggtt ggttcgcgtt ggcccggtaa gcctacggtc ggtctggtcc tgggaaccgg
     8161 cttgggcgca ctcgcgcgcg agatcgaggc cgaggcgacg attccttacc cggaaatccc
     8221 gtattttccc cggtcaaccg tcgagagcca caagggacaa ctggtgtgtg ggactctcgc
     8281 cggcaagtcg gtcctggcga tggaagggcg gtttcacctc tacgaagggt actcgccgcc
     8341 acaagtgacc tttccgattc gcgtgatgaa ggaacttggt tgccggctct tgatcgtctc
     8401 caacgccgct gggggattga atcctctgac cagcaagggg gacttgatcg tcatcgaaga
     8461 ccacatcaat ctgatgggtc tcaatcctct gatcgggccc aacgacgacc ggctgggccc
     8521 tcgatttccc gacctgatcg agccctacga ccgcgaactg caaagcctgg ccctcaaaat
     8581 tgcgctcgag aagaacatcg ttgcccataa aggtgtctat gttgctgtgg ttgggcccaa
     8641 cctcgagacc cgagcggaat atcggttcct gcgcagcatt ggggccgatg tcgtcggaat
     8701 gtcgaccgtg ccggaagttt tggtggccgt ccacgcaggc atgaaggttc tgggtttttc
     8761 gatcatcacc gacatgtgcc tgccagatgc actccagccg gtccgaatcg aagagatcat
     8821 tgccgtggcc aacctggcgg aggccaagct tcggacggtg gtcaccgggg ttctcgagcg
     8881 ctggaacggc tgatcgcgtg tgatcaagtt cgacgatgac cgcggccgat cgttgggctc
     8941 tctgccggac gaccatgacc aagagaccag cgtagcgcgt ggctctcacg cgtgccgctg
     9001 tactaaaatt taccaggtct gtgatacgcc atgtctacca aatccaagcc atacaaagaa
     9061 acgctgaacc tgcctgtaac ccgcttcgac atgaaggcaa atttgcccgt ccgagagccg
     9121 caaatccagg ctcggtggct agagcaaagg ctctacgagc aggtccgcca ggccaaggcc
     9181 gaccagccgc ggaaggtctt gcacgacggt cccccatacg ccaacggcga gatccacatg
     9241 gggaccttgc tcaacaaagt gctcaaggat ttcgtggttc gatcgctgac gatggccggc
     9301 tttgacagtc cttacgtgcc ggggtgggat tgtcacgggc tgccgatcga acacaaggtg
     9361 gtcaaggaac tggggtcaaa ggcagccgcg ctcaaccacg ccgagatccg tgcgttgtgc
     9421 cagaccgagg ccatgaagtg ggtcgacgtt cagcgcactc agttccggcg acttggcgtc
     9481 atgggtgact gggataaccc gtacttgacc ctcgatccga gatacgaggc gggcatcctc
     9541 gatatcttgg ccgagctcgt cgaggggggc tatgtcggcc gacaaaaaaa accgatccac
     9601 tggtgcatga ccgaccgcac ggcgctcgcc gaagctgagc tcgaatatcg ggaggaaacg
     9661 acgcccagca tctacgtgaa tttcccgctc gtctcaggcg tcccgcaggg ctggagcggg
     9721 tctggtacgt ggcacgcgat gatctggacg accacgccct ggactttgcc cgccaatgtc
     9781 gccatcgccg ttcatcccga gctcgagtat gtcggcgtgc gctacgttga tccagccacc
     9841 ggccatgccg ttcagacgat catcgcggcg gagctcgtcg ccaaggtcat gggcctgcgc
     9901 caggtggccg atttcgccga ggttggccgc tgccgtggca gggagctcga gcattccgaa
     9961 tatcgccacc cgttcgttga ccgcgtcagc cccattgtgc tggcgaatta cgtcagtgtc
    10021 accgatggaa ccggactggt acacactgca cccggtcacg gagccgagga ctatcagacc
    10081 ggtcgagtct accggctgtc catactgagc cctgtggacg agtcgggccg gttcacgtcc
    10141 gacgcccccc tttggctgac cggccagcac gttttcgcag cgaaccgggt catcatcgat
    10201 cggctcaagg ccggggccca tctctatcac gagcagcagc ttgaacatag ctatcctcac
    10261 tgttggcggt gcaagaagcc ggtgatcttc cgggcaaccg aacagtggtt tatctcggtt
    10321 gatcacgacg acctgcgtgg gcggaccttg aagtcgatcg atgaagtttg ctggcttccc
    10381 gagtggggcc aaacgcgcat cgaggcgatg gtctcgctcc ggcccgactg gtgcatcagc
    10441 cgccaacggt cgtggggcgt cccgatcccg gctttgggat gtaccgcctg ccacgcgcaa
    10501 ttgctcacgg ccgagaccgt ccgccatttc cgtgacctgt ttcgaaccga aggagccgac
    10561 gtctggttta cccggccggt cgagcagttg gttcctcctg gcgccgtatg cgcccattgc
    10621 ggcggcacgt cgttccgtaa ggagggagac atccttgatg tctggttcga gtcgggctcc
    10681 agccatcgca gcgttctggc gaaggaattc aacctcggct tccccacctt catgtacctg
    10741 gagggctcgg atcagcaccg cggctggttc cagtcgtcga ttctgacgtc ggttggtact
    10801 accggcagag caccattcga aacggttctg acccacggtt tcgtggtcga cgacaagggc
    10861 cggaagatct ccaagtccga ggggaattac attcccgcgg agaagatgac cagccagtat
    10921 ggcgccgacg tactgcggct ttacgtcgcc agcatggatt atgccgacga catcagcgtc
    10981 agtgagcgcg gaatcaagga gatgtcggaa gcctatcgca agatccgcaa cacattccgc
    11041 tatctcttgg ggaacctcga cgattaccgg catttcaacc ccgaaaccgt cgacctgact
    11101 tggctgcatc cgatcgaccg ttgggctctc aggcaactta acaaggtcgt ccgcgacgtc
    11161 cgggctgcct acgaacggtt tgagttttat cgcgtccatc agcggattta ccagttctgc
    11221 gctgtcgagc tgtcgagctt ttacctcgac gtcttgaagg accggcttta cgccgaggct
    11281 cccgcgggcc ccgaccgccg cgccgcgcag tttgtgctcg cccggctaca cgatcatctg
    11341 gctcgcctgc tggccccgat catcccgcac accgccgagg aatcgtggga ttacttgccg
    11401 gcgacctccg caaaggcgtc gagcgtgcac ctcgccgaat ttcccgagcc cgaccgcgcg
    11461 tgggacgacg acgccgacga tgcccgctgg gacggtttgc tcaagctccg cgagcaaatc
    11521 ttgatcgcgc tcgaaggtct gagaaagagc aagcagatcg gcagcgcgca agaagccaag
    11581 gtgagcgtca ctacaaacag gccggaacac tggctacccg atggcgagct gctggccacg
    11641 ctttgcaatg tttctgaagt ggagattatg ggtgactcga cagccaccat cgaagccgtc
    11701 acggccgagc atgcctccta tgccaagtgc gagcgttgct ggaattaccg tccgacggtc
    11761 gggcaaagcc ccgagcatcc gacactgtgc gagcgctgcg tgcgggtact gtcggagctt
    11821 gatggtggcc gtgcaactga gtgatttctc ggctacgata atcaagaacg gcactcgcgc
    11881 atgaacttag ggatgccact acgctgcggg agaatcgaac atggccacca cgaccttgat
    11941 ggacgaaatc gacatcgcca acgaaatcga ggtcgatcgt ctcgatgacg agctctacga
    12001 agtcgttgac ggcaagcgga taagaagact tcccatgagc gcatattccg ccacgatcgc
    12061 gtcccggctg gtgagaaagc ttggcatgtt caccgacgaa aggggattgg gagaaaccgt
    12121 cggggaggtg ctttttcgac tgcctttgct ggctgattta ttccgcaacc ggcgtccaga
    12181 cgtcgcgtat gtttcgtttg gccgctggcc ttcgggtcgc gcgatgccgc taaaggataa
    12241 cgcatgggat gtcgttccca atctcgcggt cgaagtgata agcactcacg atttggccga
    12301 agagtcgttg caaaaaattg gcgaatattt cgaggcaggc gttcaactcg tttgggtcgt
    12361 ctatccccaa cagcgcctgg tctatgtcta ccaaactcct actcgcgtcc acattttggc
    12421 cacggacgac accctggacg gcggggcagt cctacctggg tttcagatgc gcgtgtgtag
    12481 cctctttgat acaatcgcgc cggcgaatta gggtgcacag gctctcgcag acttcaccag
    12541 ccccacgcgc gagccccccc gatgcccgag acacagtgat tatggggagg ttccccgttc
    12601 gacaagaaca ctcctgagaa agttcttaat cccttgatgg ggctccgcgc ctgagacgac
    12661 gcgaatgtgg cgcggcagga gcttgagggc ggccctttcc gagagccgat cgccttctcc
    12721 atcgatcgcg ttgacgatca atccttgagc gctgatcgaa gagagtaact gcggcaagtc
    12781 ccacgagtgc aagacgtcga cgacgaaaaa cgacgccggg atttcccggt cgaacgacgt
    12841 tgtccggttg ccatcggccg gcatgagtcg gaacgattcg ctcagcgctc tcggccgttc
    12901 gaaaaacgca cgataactca ggaagacgtc gcgcagaaga aaccaattca actgcgattg
    12961 acgatgtagg tttgaagggc cttgcgtgat cccggaaggg taaggctccg tccgagccgg
    13021 cttgcgccgt ggctcgcccg gaggcgcgcc ttcccgggat cgtgcaagcg cagtgagctg
    13081 ccgatgggcc atcgcgtaaa cggccatcag agacgcattc ggaccgcgtg catagaggcc
    13141 gatcggtttg cccgcaaagg cgtcggtggt gcccaagtta tcgatcacct gctggaggtc
    13201 gaatgcctgt cgccccacga agttctcgcc gagcaagaga ctgacggcga agatccagcc
    13261 ggtctttgcg ctcgacgatt cgccgatgcc acgcgggtcg acgccgcaga ctgcccaacg
    13321 actttcgatt gccgcggcga tcaccggatc ggaaccgatc gattccttgc cgttgtcggc
    13381 gacggccacg atgatgcccc gggcattccc gggggcgttc agcagaaacg ccgggacatt
    13441 gatcccgggt tccgtcggga tcatgagtcg ttggagttgg acgggctcga cccgaaggcc
    13501 ggtttgctcg cgctctagcg ccggccaagg gcccaaaacc gattcaaggc tgagccgccg
    13561 atccggtgaa ttcacaggca aatcgtgggc gaggcgctca accatggcga cgatcgccgg
    13621 cccggcaggc tgattctgac cgggagggaa gcagcgcagc tccaggtcat cgaaccgctg
    13681 catttcggtc ggcggctcag ggaaggggct gccatccccc ttgtccatca gccagcggag
    13741 gaaccagccg tag
//
LOCUS       DATAQL010000205         4275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2312074, whole genome shotgun sequence.
ACCESSION   DATAQL010000205 DATAQL010000000
VERSION     DATAQL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312074"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VHS97_03510"
     CDS             complement(<1..325)
                     /locus_tag="VHS97_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3447290.1"
                     /translation="MALSNEVYPEVYTKVIKVLAQSLGVEEDDIKPSATLQADLGAES
                     IDFLDIVFRLERECGIKIPRGELFSERVFELSTKIVQDGHVTAEGLAALRSQMPYADL
                     TTLERD"
     gene            complement(395..1471)
                     /locus_tag="VHS97_03515"
     CDS             complement(395..1471)
                     /locus_tag="VHS97_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3447291.1"
                     /translation="MIGVTRCSWAAGLMLILTAHCAAGDSLGVAINSDRTTARPPREE
                     LSGQDPGRLETRRPYERGKVPVVFIHGLWGSPRNWDRMIEDLEDDPALRSKFQFWTVR
                     YTSGDSIPYSAHLLRQSLRRARRVFDPEGTDVAFDRMVVVGHSLGGILAKMMVQRSGP
                     QLWQTVCTRPIDQVIGPPEEYQFVRQAFFYKPVPEVRRVIFIATPHRGSPLASGRLRE
                     LGTQLCWRPNRFRQAHEVLLSKNGPDMFAHGFRDQLPTSAGELSPGHPLLLKLCDLGI
                     DSSVRSHSVIADLHDPPKSDGTDGIVPYSSSHLDGVASELLVHGLHICLNHPAVIEEV
                     RRILMEHLGNESGFPIDEQLSRRP"
     gene            complement(2040..3488)
                     /locus_tag="VHS97_03520"
     CDS             complement(2040..3488)
                     /locus_tag="VHS97_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3447292.1"
                     /translation="MANLLLIDDDPDLLRDQVGHVFPTPPHRVEIALTGAAGLERVAI
                     ACPDVILLDLRLPDQSGLDVLTQLRQVDARIPVVFVTVVRSADSAIEAMRHGAYDYLL
                     KPIDLQKLDRVISEALKVARLMREPAVVTEIPTDEEVPGEAIIGSCPGMQEAYKAIGR
                     VADQTFPVLITGESGTGKELVARAIYQHGPRAKAPFLALNCAAIPENLLESELFGHEK
                     GSFTGADRRRIGKFEQCSGGTLFLDEIGDMPPATQAKMLRVLQEQTFERVGGNQTIRT
                     DVRLIAATHRDLKAWSEDGKFRPDLYYRLSVFTIHLPPLRERGDDLSLLIRHFLRRFG
                     RELGRDVRQAAPEALERLRAYSWPGNIRELQSVVKQALLRAGGNILLPAFLPQSLGGL
                     AVSPPGPSGKEPAVEEFLVRLRMGPDVRDLYAEAHRDLDRLLLPRVLEHTGRSQHQAA
                     RLLGIARQTLRLKLRDLGLSAPRPADAEGDPG"
     gene            3569..>4275
                     /locus_tag="VHS97_03525"
     CDS             3569..>4275
                     /locus_tag="VHS97_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3447293.1"
                     /translation="MIRSDEIGDLRQAEQELRERERQLDSLMGHLPGLAYRALADEHW
                     TTLFVSKGTEDVTGYPPDDFTSRRINYIDIMLPEDRLATRDVILNAVRDRGTYEDEHR
                     IRHKDGSIRWIWARGHGVFAADGSLRFLEGLNLDITARKQAEEALRESEERFRGTFEN
                     AAVGIVHNDPAGRFLRVNEKYCAIVGYSREELLQKTLHDIIHSEDLAAHIELYTLCFT
                     RGESPAYPLERRYLRKDG"
BASE COUNT          806 a         1282 c         1413 g          774 t
ORIGIN      
        1 ggtcgcgctc cagcgttgtc aaatccgcgt agggcatctg cgagcgcagc gcggcgaggc
       61 cctcggcggt cacgtggcca tcttgcacga ttttggtgga gagctcgaaa acccgctcag
      121 agaacaactc accgcgcgga attttgatcc cgcactcgcg ctcgagtcgg aagacgatgt
      181 ccaggaagtc gatggattcg gcgcccagat cggcttgcaa ggttgccgag ggcttgatgt
      241 cgtcttcctc gactcccagt gattgcgcaa gcacctttat tacctttgta tagacttctg
      301 gatagacttc gtttgacaat gccatcggct gcttccttca caagatgttg ggggcgaaaa
      361 aagtcgtgat tgcttctacg ggtcgcgtgg cgctttatgg tcggcgagac aattgctcat
      421 cgatcggaaa accggattcg tttcccaagt gctccatgag gatgcgccgg acctcttcaa
      481 tgacggcggg atgattcaag caaatgtgga ggccgtgtac gagcaactcc gaggcgacgc
      541 cgtcgagatg cgagctcgaa tagggaacga taccgtcggt gccgtcggac ttgggcgggt
      601 catgaagatc ggcaatgacc gaatgagagc ggaccgacga atcgatgccc aggtcgcaga
      661 gtttcaagag cagcggatgt cccggggaaa gctcgccggc gctggtcgga agttgatcgc
      721 gaaatccgtg agcaaacatg tcgggcccgt ttttcgacaa aagcacttca tgggcttggc
      781 ggaagcggtt tggtcgccaa cacagctgtg taccaagctc tcgaagacga ccgctggcaa
      841 gcgggctgcc gcgatggggg gtggcgatga agatgacccg gcgcacctct ggcacaggct
      901 tgtagaagaa cgcctgccgc acgaactggt actcctcggg cgggccgatg acctggtcga
      961 ttggacgcgt gcagacagtc tgccagagct gcggtccgct cctctgaacc atcatctttg
     1021 ccaagatgcc cccaaggctg tggcccacga cgaccattcg gtcgaaggcc acatccgtgc
     1081 cctcgggatc gaagacgcga cgcgcccgcc gcagagactg ccgcagcaag tgggccgagt
     1141 agggaatcga gtcgccgctg gtatagcgaa ccgtccagaa ttgaaatttt gaccggagag
     1201 ccgggtcgtc ctcgaggtcc tcaatcatcc ggtcccagtt tcgcgggctg ccccagagac
     1261 catggataaa gacgacaggg accttgcccc gctcataagg ccggcgtgtc tcgagccggc
     1321 ccgggtcttg ccccgacagt tcctcgcgcg gcggacgggc tgtggtgcga tcagagttga
     1381 tcgcgactcc gagcgaatca ccggccgcgc agtgtgcggt caggatcagc atcagtccgg
     1441 ccgcccagga gcaacgagta acaccgatca tctagggctc cttcagtgtc attgagcctc
     1501 gcttggaggc tctcaccgct tgcaattcta ctaagcgtaa cacccggtca tcgggcgcgg
     1561 tccggtcggc tgactcgtcc atcccggcga tggggcaccg ggccgacgac tgctcgctca
     1621 acctgatcga cctgcggaag ccggaccgtg tgatttcttc ctctggtttg gtgcagcatg
     1681 tctcggtcgg cggcgtaccc gatggcctgg ccgtcaggat cgcgcgtcaa atatcagtgt
     1741 cgcgccggcc acgaagtcgg cgttgggctg gatcaagaaa ggcgagcggt aatgcaacag
     1801 gtgtgccaga ggtcggatgt gatcatcgca ggcgattaac cgatcgccac accgccaggg
     1861 atttcaccgc cattagatga gagtggggca ggcgctagac ggagtctaca gaaagcggcg
     1921 gccaactgga caattgacgt gaggtgacaa cttttttgcc cggttgatgg gaacatctac
     1981 ccaagccgca gatcccgtcg gcaagcgtgg ttgccgttca gcaagtgagc agggagatcc
     2041 tatccagggt ctccttctgc atcggctggg cgcggggcgg aaagtcccag gtcgcggagt
     2101 ttcaggcgca gggtctgccg ggcgattccc aatagccggg cggcctggtg ctggctccgc
     2161 ccggtgtgct cgaggacgcg gggcagcagg agccggtcca ggtcgcggtg tgcctccgcg
     2221 tagaggtcgc ggacgtcggg acccatccgc aggcggacga ggaactcctc gacggcgggc
     2281 tctttgcccg acgggcccgg gggggatacg gccaggcccc cgagggattg cggaaggaag
     2341 gcgggcaaga ggatgttgcc accggcgcgc agcagggcct gcttgacgac gctttgcagt
     2401 tcacggatat tgcccggcca cgaataggca cgcagccgct ccagcgcctc tggggcggcc
     2461 tgccgcacgt cgcgccccag ttcgcgaccg aatcggcgta gaaaatgccg gatgagcagc
     2521 gacaggtcgt cgccccgctc gcgcaatggg ggcaggtgga tggtgaaaac gctcagccgg
     2581 tagtacaggt ccggccggaa cttcccgtcc tctgaccatg ccttcaggtc gcggtgagtc
     2641 gccgcgatca gccgcacgtc ggtgcggatt gtctggttgc ctccgacgcg ctcgaacgtc
     2701 tgttcttgga gtacacgcag catcttggcc tgggtggccg gcggcatgtc gccgatctcg
     2761 tcgaggaaca gcgtgccacc cgagcactgc tcgaacttgc cgatgcggcg gcggtcggcc
     2821 ccggtgaagg agcccttctc gtggccgaac agttcgctct cgagcaaatt ctcggggatg
     2881 gcggcgcagt tgagggccag gaacggcgcc ttggcgcggg ggccgtgctg gtagatcgcc
     2941 cgggccacca gctccttgcc ggtgccactc tccccggtga tcaacacggg aaaggtctga
     3001 tccgcaacac ggccgatggc cttgtacgct tcttgcatcc ccgggcagct gccgatgatc
     3061 gcttcgcccg gaacctcttc gtcagtcgga atctctgtaa caacggccgg ctcacgcatc
     3121 aaccgggcga ccttgagcgc ctcgctgatc acgcggtcca gcttttgcag gtcgatcggc
     3181 ttgagcaggt agtcgtaggc cccgtgcctc atcgcctcga tggccgagtc agccgaccgg
     3241 accacggtga caaagacgac cggtatgcgg gcgtcgactt ggcggagttg cgtcaagaca
     3301 tcgaggccgg actggtcggg cagccgcagg tcgaggagaa tcacgtccgg gcaagcgatg
     3361 gcgacccgtt ccaggcccgc tgcgcccgtg agggcgatct caacccggtg tgggggggtg
     3421 gggaacacgt gaccaacttg gtcgcggagc aggtccgggt cgtcgtcgat caggagcaag
     3481 ttcgccatgg actgcgagcc ccacgcgctg taaacagatc tcgagcgcgg tttacattgt
     3541 acgaatacgt cacctatcgg gccatgacat gattcgatcc gacgaaatcg gcgacctccg
     3601 gcaggcggag caagagctgc gggagcggga gcggcagctc gactcgctca tgggccacct
     3661 tccgggactg gcctaccgcg ccttggccga cgagcattgg accaccttgt tcgttagcaa
     3721 ggggactgaa gacgtgaccg ggtacccgcc cgacgacttc acgtcccgtc ggatcaacta
     3781 catcgacatc atgctgcctg aagaccggct ggcgacgcgc gacgttatct tgaatgccgt
     3841 gcgggatcgc ggcacgtacg aggacgagca ccgcattcgg cacaaggacg gctcgatccg
     3901 ctggatttgg gcgcgcggac atggggtatt cgccgcggac ggatcactcc gcttcctgga
     3961 ggggctgaat ctggacatca ccgcgcgcaa gcaggccgag gaggcgctgc gcgagagcga
     4021 ggagcggttc cgcggcacgt tcgagaacgc cgccgtcggc atcgtccaca acgaccccgc
     4081 cggcaggttc ttgcgcgtca atgaaaagta ctgcgccatc gttggctact cgcgtgagga
     4141 actgcttcag aagactttac atgacatcat ccactcggaa gacctggcag cccacatcga
     4201 gctttatacg ttgtgcttta cgcggggcga gtcgcccgcc tacccgttgg agaggcgtta
     4261 cctgcgcaag gacgg
//
LOCUS       DATAQL010000206         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3839381, whole genome shotgun sequence.
ACCESSION   DATAQL010000206 DATAQL010000000
VERSION     DATAQL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3839381"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /gene="ahcY"
                     /locus_tag="VHS97_03530"
     CDS             complement(<1..1337)
                     /gene="ahcY"
                     /locus_tag="VHS97_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX3447294.1"
                     /translation="MTAAPATGDVKDLSLAEEGRKRIEWAERDMPVLRAIRERFEKEK
                     PLAGMKMSACLHVTAETANLAKTLKAGGADLLLCASNPLSTQDDVAASLVKNYGVATF
                     ASKGEDEHSYYRHIAAALQHQPNVTMDDGADLVSAMIFIALDRLDDLHNEVRKWAATL
                     TAPKRKELVANVVASMEETTTGVIRLRAMEKDGVLKLPVMAVNDAQTKHFFDNRYGTG
                     QSTIDGVIRATDTLLAGRQVVVCGYGWCGKGVALRARGMGSHVIVTEINPIRALEAAM
                     DGFQVKPMAEAATLGDIFITVTGDIHVIRSEHFAKMKDGAMICNSGHFNVELALEDLA
                     KLAVTTNKNIREFVDEYVLQNGHRLYVLGEGRLINLAAAHGHPASVMDMSFAAQALAT
                     EWAVKNKGKLEHKVYQVPKEVDEWVAALKLKTMGISIDTLTSEQKKYLESWEMG"
     gene            complement(1497..1805)
                     /locus_tag="VHS97_03535"
     CDS             complement(1497..1805)
                     /locus_tag="VHS97_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447295.1"
                     /translation="MERDRLGRRRSPEQERGLRPLHPPLPEEWISKALRVAMSEADWA
                     RFESLVARYGAMAPTQARAQGLVISDLLHAVPRPEPGPLDWMDWERRKTIRLLVPNRA
                     "
     gene            2065..2634
                     /locus_tag="VHS97_03540"
     CDS             2065..2634
                     /locus_tag="VHS97_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX3447296.1"
                     /translation="MVPAKDFKRRMVVEIDGAPHMIEHIQVQTPSARGAATLYKIKAR
                     NLKTKTRTEKAYRGTDALNESSFERRPIQFLYRDPESLHFMDSESFNQFSLPLDELRD
                     QVPYMSENMEGVDALVVDDEVIAIELPDTVELLIVETAPGVRGNSATGRTKPATLTTG
                     VVVQVPEHLDQGVTVRVDTRTGEYLGRAG"
     gene            2676..>3163
                     /locus_tag="VHS97_03545"
     CDS             2676..>3163
                     /locus_tag="VHS97_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447297.1"
                     /translation="MEGASAGYHIDARTRWLRISRLFQIRLSRLMVLIAIAAVVLLAW
                     LFNREYGSDQQAWTSSQIVALHDSDAARRRQAAENLYRVELENLSRTVVALAGALADP
                     DWQVRRAAARSLPRAIRAAVVWSGGIPKDDLTGDIDLAVRALIPAFRDPRDEVRIEAQ
                     QA"
BASE COUNT          576 a          951 c          985 g          651 t
ORIGIN      
        1 cccatctccc agctttcgag atacttcttc tgctcactcg tcaaggtgtc gatcgagata
       61 cccatggtct tgagcttgag ggcggcaacc cattcgtcga cttccttggg cacctggtac
      121 accttgtgct cgagcttgcc cttgttcttg acggcccact cggtggcgag ggcctgggcg
      181 gcgaagctca tgtccatgac gctggcagga tggccgtggg cggcggccag gttgatcagc
      241 cggccttccc ccagtacgta gaggcgatgg ccgttctgga gaacgtactc atcgacgaac
      301 tcgcggatgt ttttgttggt cgtgacggcg agcttggcca ggtcttcgag ggctagctcg
      361 acgttgaaat ggcccgagtt gcagatcatg gcgccgtctt tcatcttggc gaaatgctcg
      421 gaccggatga cgtgaatatc gccggtgacc gtgatgaaaa tgtcacccag cgtggccgcc
      481 tcggccatcg gcttgacctg gaagccgtcc atcgcggcct cgagagcccg gatcgggttg
      541 atttcggtga cgatgacgtg cgagcccatg ccgcgagccc gtagcgcgac ccctttaccg
      601 caccagccgt agccgcaaac gaccacctgg cggcccgcca agagcgtgtc ggtcgcccgg
      661 atcacgccgt cgatcgtgct ctggccggtg ccgtagcgat tgtcgaagaa gtgcttggtc
      721 tgggcatcgt tgacggccat gaccggcagc ttgagcacgc cgtctttttc catggcccgc
      781 agccggatga cgcccgtggt ggtctcttcc atgctggcca cgacgttggc cacgagctct
      841 ttgcgcttgg gtgcggtcag ggtcgctgcc cacttgcgga cctcgttgtg gaggtcatcg
      901 agccggtcga gcgcgatgaa gatcattgcg ctcaccaagt cggcgccgtc gtccatcgtg
      961 acattgggtt gatgttgcaa tgcggccgcg atgtgccggt aatagctgtg ctcgtcttcg
     1021 cccttgctcg cgaaggtcgc gactccgtag tttttcacga ggctcgccgc gacatcgtcc
     1081 tgggtcgaca gcggattcga ggcacacagc aagagatcgg caccaccggc cttcaaggtc
     1141 ttggccaggt tggcggtttc ggccgtaacg tgtaaacagg ccgacatttt cattccggcc
     1201 agcggttttt ctttctcgaa ccgctcgcgg attgcgcgca gaacgggcat gtcgcgctcg
     1261 gcccactcga tacgtttgcg gccctcttca gccaacgaaa ggtctttgac gtccccggtg
     1321 gcgggggcgg cggtcacgct tgccaattca aatactcctt acgaatttga aaaactgaat
     1381 tcccggttcg atctcgcgct ctcaaatttc gaggggcgat acgcaccctt cgtgagctta
     1441 ggtgataaac gcccagcggt gggggcaccg tcccggaccg gcgtggatcc gatccgtcag
     1501 gcgcggttgg gcacaagcaa gcggatcgtc ttgcgacgct cccaatccat ccaatcgagt
     1561 gggccgggct cgggccgagg taccgcgtgg agcagatccg aaatcacaag gccctgcgcc
     1621 cgggcctggg ttggagccat cgccccatag cgagcgacca atgactcaaa acgtgcccag
     1681 tcagcttcag acatcgcgac ccggagagcc ttgctgatcc attcctcggg caagggaggg
     1741 tgcaaaggac gaagccctcg ctcttgctca ggactccgcc gccggcctaa ccgatcgcgc
     1801 tccatgcctg ctacccattc tcggccgcga tgaactaccc gatctcggaa accaacgtcg
     1861 gagacgagtc tagcacacgc gtacgcgtgt atcaagaggt agtattccgc aaatggtctg
     1921 gttggaagtc tcgtgcggag tgcgggcgaa gttatgctac ggagattttg acctcgcaac
     1981 gccaggaccg gtaagatcga ggctgatatt cgggtgacgg ggctaagccc ttcggacttt
     2041 tgacacgagg gagggacctt ctccgtggtt cccgccaagg acttcaagcg ccggatggtt
     2101 gttgaaatcg acggtgcgcc gcacatgatc gagcatatcc aggttcaaac cccctcagct
     2161 cgaggcgcgg ccacgctgta caagatcaag gcccgcaact tgaagaccaa gacgcggacc
     2221 gagaaggcat atcgagggac cgacgcgctc aatgaatcga gctttgagcg ccgaccgatc
     2281 cagttccttt accgcgatcc ggaatcactc catttcatgg attcggagtc gttcaatcag
     2341 ttctcgctgc cgctcgatga gctgcgcgac caggttccct acatgagcga aaacatggaa
     2401 ggggttgatg cgcttgtcgt cgatgacgag gtgattgcca ttgagctgcc cgatacagtc
     2461 gagttgctga tcgtcgagac ggcacccggc gtccgcggca actcggcgac cggtcgtacc
     2521 aagcctgcta cgctcactac gggggtcgtg gtgcaggttc ccgagcatct cgaccaaggg
     2581 gtgacggtgc gggtggatac gcggacgggc gagtaccttg gccgggccgg ctagcggcgg
     2641 ttttcgaaat tgaccaatca aggtgggcac cggcaatgga aggggcgtcc gcgggttacc
     2701 atatcgatgc gcggacgcga tggcttcgta tcagccgact cttccagatc cggttatcgc
     2761 ggctcatggt gctgatcgca atcgccgctg tcgttctgtt ggcctggttg tttaaccgcg
     2821 aatacggaag cgaccagcaa gcctggacga gcagtcagat cgtcgcgttg cacgattctg
     2881 atgcggcgcg gcggagacaa gcggcggaga atctctaccg cgtcgagctc gaaaaccttt
     2941 ctcgcaccgt cgtcgcgttg gccggcgctc ttgccgatcc tgattggcag gttcgtcgtg
     3001 cggctgcgcg gtcgctccct agagccattc gcgccgccgt ggtatggtcc ggtggaatcc
     3061 ccaaagacga cctgaccgga gacatcgacc tggcggtccg ggccttgatt cccgccttcc
     3121 gagaccctcg cgacgaggtt cgcatcgagg cccagcaagc ctt
//
LOCUS       DATAQL010000207         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5060800, whole genome shotgun sequence.
ACCESSION   DATAQL010000207 DATAQL010000000
VERSION     DATAQL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5060800"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VHS97_03550"
     CDS             complement(<1..348)
                     /locus_tag="VHS97_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447298.1"
                     /translation="MIAFAMMAVAAWWLVDQPKRRGKAIATTKRAGGFVQFDDEYNPE
                     RLASPPSPISLVGLSDKWLGPEFAHGLSVVSVDGQPISEDDLTNLERVEGLRRLYLNG
                     TPITDAGMAHLGGL"
     gene            complement(409..1092)
                     /locus_tag="VHS97_03555"
     CDS             complement(409..1092)
                     /locus_tag="VHS97_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447299.1"
                     /translation="MNMISVPEVIERLAAFYGEPDPPEGTDPWEIIVWENVAYLVDDD
                     SRRKAMGSLRAKIGIRPEQIMAATSVQMLEAGVRGMVPEQSVKKLRRCAEIALHEFGG
                     DLRMILKCPLAQAKKALKRFPSIGDPGAEKILLFCRAFPILALESNGLRVLVRLGHGE
                     EKLNYATTYRLVQEAIEDELVKEYSWLIKAHQLLRRHGQELCRRSKPRCENCPLLTEC
                     PWVIGHGDP"
     gene            complement(1172..4375)
                     /locus_tag="VHS97_03560"
     CDS             complement(1172..4375)
                     /locus_tag="VHS97_03560"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX3447300.1"
                     /translation="MPTAISSQTRGSGTSDNLVDHRVAAFCSNRYPELFHTVAYANDI
                     WKHDPFDVESIHRNAREKFGRIVGRVLEPSGLASGRILLLLGESGCGKTHLMRAVRNQ
                     IHSQSSGYCGYLQMTAFTGHYGRYVLNNLIDSLDRPYDESRSETTGLMRLSNALAESC
                     EDVPRDRIDQLRERGRDQGGIDQLVGELADSIIVDERFSAIDVYLIQALLYLQRNDPP
                     VKARVLKYLRCEDLTEHDRRILGGIVPCTDPDGPHRIIERLGRLIWAVEQVPLLICVD
                     QLEDVFDLDEAAIKFRRAMATLCDIVSRLPSAIVVIACLDNFYDQLKKLLTRPIVDRV
                     ENDPPPVALGGLCNPDEVENLIGQRLKFLYESAGVSFQADLATYPLPDSLVRKLAGLR
                     PRDVLGEVHRYRERCVEKGKMAEYPFEGDGPVEPKPSLAIIPLEQAWNEFHSAFTTVV
                     PVDEAELATLVAEAIRTSSEELETGQKFEAKADGRMVEVECHGENQSLTRSLLGVCNK
                     APQGGALGRQIDEVVKRAGERAPVIVRSTDFPSNPKAAVSRQIGELITAGGRRTVVQD
                     SDCRAMMALARFRHERGADPSFSAWLKRTRPLTSLNSMRTILRLDHPKEAPVTMPAPV
                     TMPASVATVNPLTSAKTSAHPSGLRLEKVVIGTTSDRHGEPVMIEPGDLTRHAAFLGA
                     PGSGKTTVALCVVEQLLLRGIPAILVDRKGDLCCYARPDMGLRDGMTGELADRAARLR
                     NEVDVALFTPRRPDGRPLSISVIPAGLGALPVLEREQAAKFTAAALAGMMNYGDRQRD
                     LSCLAILRKAIELLSQERPTAAVPIKVLTDFVAEKDRGLVSAVGRLDLKLFDALVQDL
                     EVLRINQGDMLSAEGEPLAIEALLGVGTHLKPGKTRLSIISTKFLGTNQDIQFWVAQF
                     LMTLGRWISQSPAPSGSLQAVLLFDEADLYLPAVRQPPTKEPMEHLLKRARSAGLGLL
                     LATQSPGDFDYKCRDNIRAWFVGQVKEANSIAKMKPMLSECRVDIAARLPSQGPGEFH
                     LIQGGDATGLKTCLSAVDPRQVPEDEIVTLARRTRAH"
     gene            complement(4388..>4775)
                     /locus_tag="VHS97_03565"
     CDS             complement(4388..>4775)
                     /locus_tag="VHS97_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447301.1"
                     /translation="LIDQEPNGATPAESAGVLPQSSPTSSSAARSPVDLRTFAEQVKS
                     AARDCRTGRFGDNKVFIAHVWKALQVDPAFRSMDLAAFKEHLAEANNVRLLDLTRADL
                     VQAMDPDDVRESEVNYMSATFHFVRI"
BASE COUNT          953 a         1481 c         1390 g          951 t
ORIGIN      
        1 caatcccccg agatgggcca ttccggcgtc cgtgatcggc gtgccattga ggtacagccg
       61 tcgcaggccc tcgacccttt caagatttgt caggtcgtcc tctgagatcg gctggccatc
      121 gacactgacg accgacaatc catgggcgaa ctcgggacca agccatttgt ccgacaatcc
      181 gacaaggctg attggtgatg gtggggacgc cagtcgctcg ggattgtact cgtcgtcgaa
      241 ctgcacaaac ccaccagctc gctttgtggt cgctatggcc ttgccgcgcc gcttgggctg
      301 atcgacgagc caccaggccg ccacggccat cattgcgaaa gcaatcatta tgctccgcac
      361 cctgatccct ggtcgtcgca ttctatcacc tccgcagtgt tacatgcgtc acggatcccc
      421 gtgcccgata acccacgggc attctgtcag aagagggcaa ttttcgcagc gtggctttga
      481 ccgccggcat agctcttgtc cgtggcgccg cagcaactga tgagctttga tgagccagga
      541 atattccttg acgagttcat cttcgatcgc ctcctggacc agtcggtagg tcgttgcata
      601 gttcagcttt tcctcgccat gtcccaggcg caccagcacg cgtaggccgt tggactcgag
      661 tgccagaatt gggaaggcgc gacagaatag caagatcttc tcggcgcccg ggtcaccaat
      721 tgacggaaac cgtttgaggg ctttctttgc ttgggccagc gggcatttaa gaatcatccg
      781 caaatcgccg ccaaactcat gaagagcgat ttctgcgcag cgccgaagct tcttcacgct
      841 ttgctctggc accatgccgc gaaccccagc ctcaagcatt tgaacggatg tggcagccat
      901 gatctgctcc gggcgaattc cgatcttcgc ccgcagcgag cccatcgctt tccgccgact
      961 gtcgtcatca accaggtagg cgacgttttc ccagacaatt atctcccagg gatcagtacc
     1021 ttctggcgga tcgggctccc cgtaaaaagc cgcaagccgt tcgatcacct ccggcacaga
     1081 gatcatattc atgacggaga ctttcacgcg aagcactggt agacgattcc agtacgagcc
     1141 ggccagactg cgaaacgccg gccgcgccgc ctcaatgggc acgcgtccgg cgggcgagtg
     1201 tcacgatctc atcctcggga acctgcctgg gatccacggc cgagagacag gtcttgaggc
     1261 cggtggcatc gcctccctgg atcaggtgaa actcgcctgg cccctggctg ggaagccgag
     1321 ccgcgatgtc gacgcggcac tcgctgagca tcggcttcat cttggcgatg gagtttgctt
     1381 ccttgacctg gccgacgaac caggctcgga tgttgtcccg gcacttgtag tcgaagtcgc
     1441 caggactctg ggtcgccaaa agcaaaccca ggccagcgga ccttgctcgc ttgagtagat
     1501 gctccatcgg ctctttggtc ggcggctgac gtactgccgg caggtacaga tcggcctcgt
     1561 caaagaggag cacggcctgc agcgaccccg acggcgccgg cgactggctg atccaccggc
     1621 ctagcgtcat gagaaattgc gcgacccaga actgaatatc ctgattggtg cccagaaatt
     1681 tcgtgctgat aatgctcagc cgggtcttgc cgggcttaag gtgcgtgccg acgcccaaaa
     1741 gcgcctcgat cgcgagtggc tctccttcgg cggagagcat gtcgccttga ttgatccgca
     1801 ggacctccag gtcctgaacc aacgcatcga acagcttcaa gtccaagcgg ccgaccgcac
     1861 ttaccaatcc acggtccttt tcggcgacga agtctgtcaa aaccttgatc ggcaccgcag
     1921 ccgttggacg ctcttggctc agcaactcga tcgcctttcg caggatagcc aggcaagaca
     1981 gatcacgctg ccgatcgccg tagttcatca ttccagcgag cgcagcggcg gtgaacttgg
     2041 cggcctgttc gcgctcgagg accggcaaag cgcccagacc ggcggggatc actgagattg
     2101 acaacggccg gccgtcgggc cgacgcggcg tgaaaagcgc aacatccacc tcgttgcgca
     2161 gccgagcggc ccgatcagcc agctcgcctg tcataccgtc gcgcagcccc atgtcgggcc
     2221 gggcgtagca gcagagatct cctttgcggt caaccagtat cgccggaatt ccacggagga
     2281 gcagttgttc gaccacacag agcgccaccg tggtcttacc gctccctggt gcgcccagga
     2341 aggcggcatg ccgcgtcaga tcgcctggct cgatcattac cggctcgcca tgccggtcgc
     2401 tggtcgtgcc gatgacgacc ttttcgagtc gcagtccgga gggatgagcg ctcgttttgg
     2461 ctgatgtcaa aggattcaca gtggccactg aagctggcat cgtcacaggc gctggcatcg
     2521 tcaccggcgc ctccttgggg tggtccagtc tcaggatcgt ccgcatcgaa ttgagactcg
     2581 tcaaaggacg agtacgcttg agccaggccg agaagctagg gtccgcaccg cgctcgtgcc
     2641 gaaacctcgc taaagccatc atcgcgcggc agtcggagtc ttgcacgacc gtccgacggc
     2701 caccggccgt aatcaattcg ccaatctgcc gcgacacggc cgccttcgga ttgctgggaa
     2761 aatccgtcga gcgcacgatc accggcgcgc gttcgcccgc tcgtttgaca acttcgtcga
     2821 tttggcgacc cagcgcaccc ccttgcggag ccttattgca gacccccagc agactgcgcg
     2881 ttagcgactg attttcgcca tgacactcga cctcgaccat ccttccgtcg gctttggcct
     2941 caaatttctg gccagtctcg agctcctcag agctagtgcg aatggcctca gcgacgagcg
     3001 ttgcgagctc ggcctcatcg actggcacca cggtggtgaa cgctgagtga aattcgttcc
     3061 aggcttgttc gagcgggata atcgctagtg atggctttgg ttcaactggg ccgtcaccct
     3121 caaaagggta ttccgccatc ttccctttct cgacgcagcg ctcgcggtag cgatggacct
     3181 cgcccaggac gtcgcgcgga cgtagacctg ccagttttcg cacgaggctg tccggtagtg
     3241 gatatgtcgc cagatcggcc tgaaaggaaa cacccgcgga ttcgtacagg aacttgagac
     3301 gctggccgat caagttttcg acctcgtctg gattacaaag acctcccaaa gcgactggcg
     3361 gcggatcgtt ctcgacccgg tccacgatcg gccgcgtcag tagctttttc agttgatcgt
     3421 agaagttatc gagacatgct atgaccacga tggctgacgg cagccggctg acgatgtcgc
     3481 aaagtgtcgc catggcacgg cgaaacttga tcgctgcctc atcgagatcg aagacatcct
     3541 caagctgatc gacgcaaatc aagagcggaa cctgttcgac ggcccagatt aaacgcccca
     3601 ggcgctcgat gatccggtgg ggcccatcgg gatcggtaca cggaacaatc ccgcctagta
     3661 tccgccggtc gtgctccgtc agatcttcac accggaggta tttgagcacg cgggccttga
     3721 ccggcgggtc gttgcgctgg agataaagga gcgcctggat cagatacaca tcgatggcgc
     3781 taaatcgctc atcgacaatg atcgagtcgg cgagttcacc caccaactgg tcgatccccc
     3841 cttggtctcg tccgcgctcg cggagctggt cgatccggtc acgcggtacg tcctcgcatg
     3901 actcggcgag cgcgttcgag agccgcatca gcccggtggt ttcggaccga gattcatcgt
     3961 agggcctgtc aagcgaatcg atcagattgt tgagcacgta ccgcccatag tggccggtaa
     4021 acgccgtcat ctgcaggtag ccgcaatatc ccgagctctg ggagtggatc tggttccgaa
     4081 cagcgcgcat taagtgggtc ttgccgcagc cggattcccc caatagcagg agaatcctgc
     4141 cgctggccag gcctgacggc tcgagcactc gccccacgat ccgcccgaac ttctcgcggg
     4201 cgttccggtg aatcgactcc acatcgaagg ggtcgtgttt ccagatgtcg ttggcgtagg
     4261 cgacggtatg gaaaagctct ggataccggt tcgaacagaa cgcggcgacg cggtgatcga
     4321 ccaagttatc cgaggtgcca gagccgcgcg tttggctcga gatggctgtc ggcataaggt
     4381 tgccctttca gatccgtacg aaatgaaacg tggcgctcat ataatttacc tcggactcac
     4441 gaacatcgtc gggatccatg gcctgaacca ggtcggccct tgtgaggtcg agcaacctca
     4501 cattattcgc ctcagcaagg tgttccttga aagcagctag gtccatactc cgaaacgcgg
     4561 gatcgacctg gagagccttc cagacatggg cgatgaacac cttattgtca ccaaaccggc
     4621 cggtgcgaca gtcgcgtgca gcggacttca cctgctcggc aaaagtccgt aaatcaaccg
     4681 gtgaccgggc ggcggaggag gacgtcggcg aggattgagg caagaccccc gctgattcag
     4741 cgggggtagc gccattaggc tcctggtcga tcaaa
//
LOCUS       DATAQL010000208         4686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3991928, whole genome shotgun sequence.
ACCESSION   DATAQL010000208 DATAQL010000000
VERSION     DATAQL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3991928"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..1511)
                     /locus_tag="VHS97_03570"
     CDS             complement(429..1511)
                     /locus_tag="VHS97_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX3447302.1"
                     /translation="MSVLNIRKDSCDLDFLSLGALIHRLDPGIIPFRKARSFDIHVSG
                     GEYNVAASLSDCFGLRTGIATAMVKYPIGELVVSRVRETGVTPYYKWFEHDGVRGPNI
                     ATVYSDRGYGVRAPVVFYNRANEAGALLKPGDFDWARIFARGVRWFHSGGIFASLSET
                     TAPLIVEGMQAARAAGAITSFDLNYRAKLWASVGGAERGQQVMKRIAGQLDALVGNEE
                     DLQKGLGIAGPEVATKSKLDPDTFFALIERVVEQYPNIKLVATTLREVHSTNKHDWAA
                     VIWLDGQRYVSPTCQLDVIDRIGGGDGFAAGLFYGLISGRPPEEALRLGWAHGALLTT
                     FPGDVTMAKLEEVEALAKGGSARVQR"
     gene            complement(1591..2712)
                     /locus_tag="VHS97_03575"
     CDS             complement(1591..2712)
                     /locus_tag="VHS97_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX3447303.1"
                     /translation="MKAVAVLPGKKNSVHSRDIPVPKLSDQPHPHVCKIADGRAVLVK
                     VLQVGVDATDREINEALYGNAPPGGEHLVIGHESFGRVIEVGDKVIEVKPGDYVSCTV
                     RRPGGSLFDKIGRNDITSEEVYYERGINLCHGYLTESFVDDAEYIVKVPQNLKHLGVL
                     SEPASVCAKAIDQAFLAQQRLQVWNPKKAFVLGAGQIGLLATMMLKLRGIEVFTLATR
                     PGPHRKSEIVEAYGATYVSTKKTAMADLAKQVGKPDLIFEATGNAEVCFRSMEVLAHN
                     GALIWTSITGGKHEVTVDAAKINLEWVLGNKLLVSSVNGNRRHFEQGIQALSHGELTY
                     PGVTERILTHPVSGLDNFREMMRLLEEKEALKVFVNVAE"
     gene            complement(2801..3955)
                     /locus_tag="VHS97_03580"
     CDS             complement(2801..3955)
                     /locus_tag="VHS97_03580"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX3447304.1"
                     /translation="MSSRIVLFTLALNLLGMPPLAAALSAVRSDAEAQSQRSLLRMDF
                     GKTPEGAPVELYVLTNGKMTAKVMTYGAILTELQVPDRNGKLADVVLGFDSLDGYLAD
                     QPHFGATTGRVANRIAKGRFTLDGTEYKLAVNNGPNSLHGGLKGFDKKVWKAVDASGQ
                     AGPAVKLTYTSPDGEEGYPGNLSVSVTYTVTPDNELKIDYLATTDKATPINLTNHSYF
                     NLAGPASGPILDHELLLAADQYTAADDTLIPTGEIKPVKGTPLDFTKPTPIGARFDQL
                     SGDPRGYDHNYVLRSDGKTPALGARVYEPGSGRVLEMFTTEPGVQFYTANFLDGTLKG
                     KGGALYGKHQGFCLEAQHFPDSIHHDNFPSTVLRPGEKYTQTTIYKFSAR"
     gene            complement(4158..4499)
                     /locus_tag="VHS97_03585"
     CDS             complement(4158..4499)
                     /locus_tag="VHS97_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447305.1"
                     /translation="MPGVAPAAMQAGWHVAGNITRTLRGQPRLPFHYVDKGMLATIGR
                     GAAVARIGPIKASGYFAWLLWLFVHILFLIGFRNRLLVMFQWAWSYVTFDRGARLITE
                     PLKEPLVASHS"
BASE COUNT          905 a         1454 c         1416 g          911 t
ORIGIN      
        1 cttcgggcac cctctccccg cccggcgggg cgagggaaaa gaacggcagc ggcacttccg
       61 aaagcatggc gagaaagaca agtcgccctc gcccgggaga gggaagaact tcgcccagat
      121 cggcgcgcaa aaaatgccgt agcggtctgc ccaaggaggt cggcgctccc gaaaccggga
      181 ttcgtcgcaa tagcgaccct tctgacgccc caggccgaac acctcaaacc tggtacttga
      241 agtacgggct aaaccaccac ggcaaccact attttgacgc actccgcgac attgtcaagt
      301 cgctgccgat gggttgcggc gcgagtggcc tcgacctcct gatccggccg cgagctgttc
      361 tggtaagtgt ggtcgccgcc cgctccatcc agcgcgacgc ttcctcgccc gctaagattg
      421 tcaggccact accgctgcac gcgggccgat ccccctttgg cgagtgcttc cacttcctcg
      481 agcttggcca tggtaacgtc gcccggaaac gtcgtgagca gtgcgccatg ggcccagccc
      541 aggcgcaggg cctcttccgg gggccgtccg gaaatgaggc cgtagaagag tcccgcggca
      601 aagccgtcgc cgccgccgat gcggtcgatg acgtcgagct gacaagttgg gctgacatag
      661 cgctgaccgt cgagccaaat cacggccgcc cagtcatgct tgttggtgga atgcacctcg
      721 cgcaacgtcg tcgcgaccag cttgatgttg ggatattgct cgaccacacg ctcaatcagc
      781 gcgaagaacg tatcggggtc gagcttcgat ttggtggcca cttccgggcc ggcaatcccc
      841 agccctttct gcaagtcttc ctcattgccg acaagcgcat cgagttgccc cgcgatccgc
      901 ttcatgacct gctggccgcg ctcggctccc cccaccgacg cccaaagctt ggcgcgatag
      961 ttaaggtcga acgaagtgat ggcgccggca gccctggctg cttgcattcc ctcgacgatc
     1021 aatggcgccg tcgtttccga aagcgacgcg aaaatgccgc cggaatggaa ccaacggacg
     1081 ccgcgggcga agatccgggc ccaatcgaag tcgcccggtt tgagcaaggc cccggcctcg
     1141 tttgctcggt tgtaaaagac cacaggcgcc cgcaccccgt acccccggtc gctgtagacc
     1201 gtggcgatat tgggtccccg cacgccatcg tgctcaaacc acttgtaata cggggtgaca
     1261 ccggtctcgc gcacccgcga cacgacgagc tcgccgatcg ggtacttgac catcgccgtc
     1321 gcgattcccg tccgtaggcc gaagcaatcc gatagcgagg cggccacatt gtattcgccg
     1381 ccggaaacgt gaatatcgaa cgaacgcgct ttgcggaagg ggatgatgcc agggtcgagc
     1441 cggtggatga gcgcgcccaa ggaaaggaag tccaggtcgc acgaatcctt acgaatattc
     1501 aaaacgctca tggctgctcc agatactttc tccaaagcct tgtctgccgc cagattgccc
     1561 gtcgagtcgt tcgtgccggc gcaacccagc tcactccgcg acgtttacaa aaaccttgag
     1621 cgcctctttc tcctccaaga gccgcatcat ctcacggaag ttgtcgagcc cggacacggg
     1681 atgcgtgagg attcgctcgg tgactcccgg ataggtcaat tcgccgtgag agagtgcctg
     1741 gatgccctgt tcgaagtggc ggcggttgcc gttgacgctg gagaccaaaa gcttgttgcc
     1801 gagcacccat tccaggttga tcttggcggc gtcgaccgtg acttcatgtt tgcctccggt
     1861 gatactcgtc cagatcaggg caccgttgtg tgcgagcacc tccatcgagc ggaagcagac
     1921 ctcggcgttg ccggtcgctt caaagatgag gtcgggcttg cccacttgtt tcgcgaggtc
     1981 ggccatcgct gttttcttgg tgctcacgta ggtggcgcca taagcttcga cgatttcgga
     2041 cttgcgatgc gggccaggcc tggtcgccag ggtaaatacc tcgattccac gcaacttgag
     2101 catcatcgtg gcgagtagtc cgatctgacc ggcccccagc acgaaagcct tcttggggtt
     2161 ccagacttgg agccgctgct gagcgagaaa ggcctgatcg atcgccttgg cacagacact
     2221 cgcgggctcg gagaggacac ccaaatgctt gagattctga ggcaccttca cgatgtattc
     2281 ggcatcgtcg acaaacgact cggtcaggta gccgtggcaa aggttgatgc cgcgctcgta
     2341 gtacacctct tcgctagtga tatcgttgcg gccgatctta tcgaacagcg atccgccagg
     2401 acggcggacc gtgcatgaga cgtaatcgcc cggcttgacc tcgatcacct tgtcgccaac
     2461 ctcgatgacc cgaccgaagc tctcgtgccc aatgacgagg tgctcgccgc cgggcggggc
     2521 gttgccgtag agggcttcgt tgatctcacg gtccgtggcg tcgacgccca cctgaagcac
     2581 cttcaccaac acagcccgcc cgtcggcaat cttgcatacg tggggatgag gctggtcgga
     2641 tagcttggga accggaatgt cccgggaatg aacgctattc ttcttgcctg gcaaaacggc
     2701 aacggctttc atcggtgacg cgcctgatcg ataaggtaca aagaaccgcg ccggctcccg
     2761 ttttgggcga aagccgggct ccaatgcgat tcgccggctt tcatcgcgcg gagaacttgt
     2821 agattgtcgt ctgggtgtac ttttcgccgg gtctcaggac ggtggacgga aagttatcat
     2881 ggtggatcga gtcgggaaaa tgctgggcct cgaggcaaaa gccttggtgc ttgccgtaaa
     2941 gggcgccccc tttgcccttc aaggtgccgt ccagaaagtt cgcggtataa aactgcacgc
     3001 ccggctcggt cgtaaacatc tcgagcaccc ggccgctgcc gggttcgtag acgcgcgctc
     3061 ccaaggccgg cgtcttgccg tcactcctta acacataatt atgatcgtag ccgcgcggat
     3121 cccccgagag ctggtcgaat cgtgctccga tgggcgtagg cttcgtgaaa tcgagcggcg
     3181 tccccttgac gggtttgatt tcgccggtcg gaatcagcgt gtcgtcagcg gcggtgtact
     3241 ggtcggccgc cagcaacagc tcatgatcca ggatcggacc ggatgcgggc ccggccaggt
     3301 tgaaatagct gtggttggtg aggttgatcg gggtcgcctt gtccgtcgtc gccaagtagt
     3361 cgatcttgag ctcgttgtcg ggcgtgacgg tataggtcac actgacggac aggttgccgg
     3421 gatagccttc ctcgccgtct ggactggtgt acgtcagctt gacggccggg ccggcctggc
     3481 ccgaagcgtc cactgccttc cagacctttt tatcaaagcc cttcaggcct ccgtgcagcg
     3541 aatttggtcc gttattgacg gccagcttgt attcggtgcc gtcgagggtg aaccgaccct
     3601 tggcgatgcg gttcgcgacg cgacccgtcg tcgcgccgaa atggggttgg tcggctaaat
     3661 agccgtcgag tgagtcgaag ccgaggacga cgtcggccag tttcccgttc ctgtccggaa
     3721 cttggagctc ggtcaggata gccccgtagg tcatgacctt ggcggtcatt ttgccattcg
     3781 tgagcacgta caactcgacc ggagctccct ccggcgtttt tccgaagtcc atcctaagaa
     3841 ggctcctctg gctctgggct tccgcgtcgg atcgcactgc gcttagtgcg gcggccaaag
     3901 gcggcatccc cagcagattg agggcgagcg taaacaacac gatacggctg ctcatggatc
     3961 ggcctcacta gttgaataac agaggatacc gcagcgctcc gcgaatagtc agtagcatgt
     4021 cggcaaaagt acgatcccgg ccacccatgc cggattgtaa cgcgccattc cgggccatag
     4081 cgcattcccc ggcggctacg agacaaaggg tcaggcccct tttctacaaa aaaggttcct
     4141 gacccctttg tctctttcta actgtgcgac gcgacaagcg gctccttgag cggctcggtg
     4201 atcaaccgtg cgccacgatc gaaggtgacg tacgaccagg cccactggaa catgaccaaa
     4261 agacggttac gaaacccgat caggaacagg atatggacga acagccagag cagccaggcg
     4321 aaatagcccg acgccttgat cggcccgatt ctggcgacgg cggcaccgcg gccgatggtt
     4381 gccagcatcc ctttgtcgac gtaatgaaac ggcaggcgag gctggccgcg cagcgttcgc
     4441 gtgatgttgc cagcaacgtg ccagcctgct tgcatcgcag cgggggcgac ccccggcacc
     4501 aaagcgccgt ctttttcgac gtgagccaga tcgcctataa cgtagacgtc gtcgcgtcac
     4561 gggatcgtca gctctggctc gaccaggacg cgaccagccc gatcgagtgg cacaccgaga
     4621 gtcactccca gcggcgaggc cgctacaccc gcggcccaga ccacggtgtg ggcgtggacc
     4681 cgctcg
//
LOCUS       DATAQL010000209         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2922548, whole genome shotgun sequence.
ACCESSION   DATAQL010000209 DATAQL010000000
VERSION     DATAQL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2922548"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VHS97_03590"
     CDS             complement(<1..643)
                     /locus_tag="VHS97_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3447306.1"
                     /translation="MSDTLADRLVAFALSTRYEDLAPEVVAEARRRLIDALGCAVGAL
                     AEPASSIARRVAARTQGVPSVGLIGGGKAAPDWAAFSNGVHIRYLDCNDTYLSLEPAH
                     PSDNWADVMAAGEAAGADGKAWITAAAVAYEVQCRLCDAASIRARGWDHTTYGSISST
                     LAAAKLLSLSHDQAVHALGIAGTTGTALRLTRAGELSMWKGCAFASAARNGLFA"
     gene            complement(742..>2484)
                     /locus_tag="VHS97_03595"
     CDS             complement(742..>2484)
                     /locus_tag="VHS97_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447307.1"
                     /translation="QKRAEAELVYALAELSWIEAKRLDHRRKPQAIDRYIDAAAYAHD
                     YLFGDDPVLAEGRGPSDPRFRLAMEIYNSGVDRLIRAAQTKGQIQPQNGEAIPFKVHG
                     REQSLRIVLKDSPWRAADVHKLLLASDFEVTGINRDLSQYGLGVPLIAVRETETKPGE
                     RPPGERFYPAEMAFPLTAFLVPNSRLHDANVNINQARECTLLLYDPVQTRAVGSRTNV
                     LSLEIDLTTPLAYMWSRTDLDRYRWTGLLRPEQALERANLLLVRPYEPHKIPVVMVHG
                     LISTPLAWIPMLNELLRDPVLQSQYQFMLYMYPTGVPIPIAAAGLRESLIQAKMMYDP
                     DGRDPKFDRMVLLGHSMGGVLSRTMAVSSGDQFWRLYSDRSFDDILGPREVLDELRGY
                     FFFEPLPFVRRVIFLATPHHGSDMSRGVVGRVGTSLISDPDHIHKLLYQLVKDNPDAF
                     DKRRFRRLPTSIETLVPDSPILTALSNMQPPAPPNVINFHSIIGSERPTGVEKTTDGV
                     VPYRSAHLDGVASEKVVRSDHGVQKDPEAIREVRRILHEHVGVMPTVPAIHESRAQSE
                     SAQAGKLEMPSTDR"
BASE COUNT          492 a          754 c          788 g          450 t
ORIGIN      
        1 ccgcgaagag cccattgcgg gccgcggagg caaatgcgca tcccttccac attgacagct
       61 ctccggcacg agtaagccgc aacgcggtgc ctgtcgtgcc ggcgatcccg agcgcatgca
      121 cggcctgatc gtgtgagaga ctcagcagct ttgccgcggc caaggttgat gagatcgagc
      181 cgtaagtcgt gtggtcccaa ccccgagcgc ggatgctggc ggcatcacac agccggcact
      241 gaacttcgta ggcgaccgcc gcagccgtga tccaggcctt gccatccgca ccagccgctt
      301 caccggcggc catgacgtcg gcccagttgt cactgggatg cgccggctcg agcgagagat
      361 aagtgtcgtt gcagtcgagg tagcgaatgt ggacaccatt ggaaaacgcg gcccagtcgg
      421 gcgcggcttt cccaccgccg atcaacccga ccgacggcac accttgagtt ctcgcggcga
      481 cgcggcgtgc aatcgaggac gcaggctcgg ctaatgcccc gacggcgcac cccagggcgt
      541 cgatcagacg ccggcgggcc tcggccacga cctcgggcgc aagatcctcg tagcgcgtgc
      601 tcagggcaaa cgcaactaaa cggtcagcga gcgtatcgga catcaatcgg cctcgttcca
      661 aatgcttgtc ctcggttgcc tggttgtcgc ccgatcctcc gaatcggggc cgtcgaccac
      721 gcgcgacaac tgaactctac cctatcgatc ggtactgggc atttccagct tcccggcctg
      781 cgcggattcg ctctgagcac gggactcgtg gatggccggt acggtcggca taacgcccac
      841 gtgttcgtgc agaatccgac gcacctcgcg gatggcctcc ggatctttct ggacaccatg
      901 atcggaccgc acgacttttt ccgatgcgac cccgtccagg tgagcgctgc gatacggaac
      961 gacgccatca gtcgtctttt cgacgccggt cggtcgctcc gagccgatga tcgagtggaa
     1021 gttgatcaca tttggaggcg caggtggctg catgttcgaa agcgcggtga ggatcggcga
     1081 gtcgggcaca agtgtctcga tggacgtcgg caggcggcga aatcgccgct tgtcgaaggc
     1141 atcgggattg tctttcacca actggtagag aagcttgtga atgtggtcgg gatcggagat
     1201 caagcttgtg cccacgcggc ccacgacccc gcgagacatg tccgagccgt gatgtggtgt
     1261 ggccagaaaa atgacccggc ggacaaaggg caatggctcg aagaagaaat aaccacgcag
     1321 ctcatccagg acctcacgcg gccccagaat gtcgtcgaac gaacgatcgg agtaaagtct
     1381 ccagaactgg tcaccgctcg aaaccgccat tgtccggctc aatacgcctc ccatgctgtg
     1441 acccagaagg accatccggt cgaacttggg atcgcggcca tcgggatcgt acatcatctt
     1501 ggcctggatc aacgactcgc gcagacctgc cgccgcgatc gggatcggta cgccggtcgg
     1561 atacatgtac agcatgaatt gatactggct ttgaagcacg ggatcgcgca agagctcatt
     1621 gagcatcggg atccaggcca gcggggtaga gatcaaacca tggaccatga cgaccggaat
     1681 tttgtgcggc tcgtagggac ggaccaacag caagttggcc cgctcgagcg cctgttcggg
     1741 gcgaagcaaa cccgtccagc ggtagcggtc gagatcggtc cgagaccaca tgtacgccag
     1801 cggggtggtc aggtcgatct cgagggaaag cacgttggtc ctgctgccga cggcgcgtgt
     1861 ctggacagga tcgtacagta ataacgtgca ttcccgcgct tgattgatat tgacattggc
     1921 gtcgtgcagc cgcgagttgg ggacgaggaa tgctgtcagg ggaaaggcca tctcggccgg
     1981 atagaatcgc tcgccaggcg ggcgctcgcc aggcttggtc tcagtttcgc gaacggcgat
     2041 caacggcaca cccagtccat actgtgaaag gtcgcgattg atgcccgtca cctcgaagtc
     2101 cgaagccagg agtagcttgt ggacatccgc ggccctccag ggagagtcct tgagcacgat
     2161 ccgcaacgat tgttcgcggc cgtgaacttt gaacgggatg gcttcaccgt tctgtggctg
     2221 gatctgcccc ttggtctgcg cggcgcggat cagacggtcg actccggagt tgtagatctc
     2281 catcgccagc cggaaccgcg gatccgaagg cccacgtccc tcggcgagca ctggatcgtc
     2341 gccgaaaaga tagtcgtggg catatgcggc ggcgtcaatg tagcggtcga tcgcctgcgg
     2401 cttacgccga tggtcgagcc gtttggcctc gatccacgac agttcggcca gggcgtagac
     2461 cagctcggcc tcggcgcgct tctg
//
LOCUS       DATAQL010000210         4929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3229219, whole genome shotgun sequence.
ACCESSION   DATAQL010000210 DATAQL010000000
VERSION     DATAQL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229219"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VHS97_03600"
     CDS             <1..638
                     /locus_tag="VHS97_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447308.1"
                     /translation="GAGGKFFVWFMANSNNAFPEANTGNNLSKPAAVQVTGQPLPALR
                     AIGLAVPSRMQPGDTINPTIVVENFGTADTSAQGPVTVDLVESVTKNFTLGSQIVATY
                     TINTLAGVSGTPTKGNFRKFAKQILAQPSNVVSLGGTKVTLSTTPSTYFLGVVIDPQG
                     AIKQLSLPKNSFQVIHVVGPPIKGLPPANTVSNGTSAQFPTAPFGGKVGNV"
     gene            894..1895
                     /locus_tag="VHS97_03605"
     CDS             894..1895
                     /locus_tag="VHS97_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3447309.1"
                     /translation="MTRRYVNQLANGDSVDDAFLVADKQLRANRQGNLYLHLELRDKT
                     GTVGARLWNATENLARQFEPGDYLHVRGKTQVFQGSLQIILTNLEVLPANQIEPEEFL
                     PQSSQNVTKLLARLREVLLAMGNPHLRALVECFLIDDVFVRKFTTAPAGIKNHHAYQG
                     GLLEHVVSLLNVADRISDLYPEIDRDILLTGIFLHDIGKVDELSYERAFGYTDPGQLV
                     GHLVMGVEMLGEKVERSSELTGEAFPAELLLRLKHMIVSHHGTHEFGSPKLPMTLEAI
                     ALHYLDNLDAKIHTFSREIRDDPSRDSSWTPFQQSLGRRLFKGNTPVDGAGDDGAEP"
     gene            1876..4239
                     /gene="rnr"
                     /locus_tag="VHS97_03610"
     CDS             1876..4239
                     /gene="rnr"
                     /locus_tag="VHS97_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX3447310.1"
                     /translation="MTGRSLEPQEWLAMADFAGRVAQLVARPDYKPITLKAMSRYFDV
                     PADDYADFRAAVKRLVKEGKLDLAKDKTLRRPEQAGVIVGLFRRSAKGFGFVRPANST
                     SKIDHIYIPAESTRDASSGDEVAVKITKRSRREGMNVEGRIVEVLARASGTFVGTYTE
                     VGETGYVKIDGTTFRDPISVGDPGAKGVKPGDKVALEIVRYPTPFLEGEGVITEILGQ
                     RGQPGVDTLTVIRAFNIPDTFDEEVLDEAREQGKRFNEDEIGERLDLRGLTTVTIDPA
                     TARDFDDAISLSRDTQGHWTLAVHIADVAHFVRPGSALDQAARHRGTSVYLPDRVIPM
                     LPEILSNSLASLQAGRTRYTVSVFMDFNPDGILTSKRFARSAIRVDHRFAYEHALEVM
                     RNPQSAHQGVAPEITGMLAQMLELAMILRRRRFARGGLELSLPEVEIELGDHGQVTGA
                     HLAVNDESHQVIEDFMLAANEAVATSLTEKDVSFLRRAHPDPEAFKLLQFADFVRSLG
                     LKLDQPQSRFELQRVLKETIGKPEEYAVHYGLLRSLKQANYTPEPEGHYALASLDYCH
                     FTSPIRRYPDLQVHRQLIAVLEGKKPRAKHDELVVLGQHCTKTERRAEAAERELIRMK
                     LLSFLEDHVGKAFHAVIVGVEDFGLFCRLVELPVDGLIHVTSLADDFYYLESGTHTLV
                     GRRSGRRHRLGDREIVRVAHVDVDRRELDLVLADDPVTRARSARTGRLATTTESHSGR
                     QGPEVRRPGRAETSAAADTESGPQRRKGPKKKKSTARAKKAGKKKRP"
BASE COUNT         1022 a         1491 c         1464 g          952 t
ORIGIN      
        1 caggggctgg aggcaagttt ttcgtctggt tcatggccaa ctccaataac gcatttcccg
       61 aagccaatac cggcaataac ctgagcaagc cggctgccgt gcaagtcaca ggtcagccgt
      121 tgccagcgtt gcgtgccatc ggtctggccg tgccgtcacg gatgcagccc ggagatacaa
      181 tcaacccgac cattgtggtc gagaacttcg gaacggccga tacctcggcc cagggcccgg
      241 taactgtcga cctggtcgag tcggtcacca agaacttcac gcttggtagc cagatcgttg
      301 cgacctacac gatcaatacc ctcgccggcg tctcgggaac gccgaccaag ggcaacttcc
      361 ggaagtttgc caagcagatt ctcgcccagc cgtccaatgt cgtgagtctc ggtggcacga
      421 aggtcactct atccacgacc ccaagcacct atttcttagg cgtcgtgatc gacccccagg
      481 gcgcgatcaa gcagctcagc ctgcccaaga actcattcca ggtgatccac gtcgtcggac
      541 cgcccatcaa gggcttgcct ccggccaaca ccgtgtcgaa tggaacctcc gctcagttcc
      601 ccaccgcgcc cttcggcggc aaggtcggca acgtctgatt gtcgttgtct cgagcagccc
      661 ggaaccgatc gagcttggtt ccgggctgct cccgggcagg tacgttcgcc cgctcatgac
      721 tttttcgctc ccaatcgcaa ggctgcgcgt gtcactgcct tgcctgcgga gcccaggcga
      781 ggagcaccgg ctcccttgac cgctgcgccg gctcgcccct acaatcgacc gctcgccggt
      841 cgccgtgccc ggccttctcg ctcttgcgtt cgttcacccg atcgaattgc ctcatgaccc
      901 gccgctatgt gaatcaactg gccaatggcg actcggtcga cgacgcgttc ttggtcgccg
      961 acaagcaact ccgggccaac cgccagggca acctctatct ccaccttgag ctgcgcgaca
     1021 agacggggac cgtcggagcg cggctttgga acgcgacgga aaatttggct cgccagttcg
     1081 agccaggcga ttatctgcac gtccggggca aaactcaggt ttttcaggga tcgttgcaga
     1141 tcatcctcac caatctcgag gtccttccgg ccaatcagat cgagcccgag gagtttctgc
     1201 cccagagctc gcaaaatgtc accaagttat tggcgcggct gcgcgaagtc ttgctggcga
     1261 tgggtaatcc acatctccga gcgctggtcg agtgctttct gatcgacgac gtgtttgtcc
     1321 gcaagtttac gacggcaccg gccggtatca agaaccacca cgcctatcaa ggtgggctgc
     1381 tcgagcatgt tgtttcgctc ttgaacgtgg cggacaggat cagcgatctt taccctgaga
     1441 tcgaccgcga cattctcctg acggggattt tcctgcacga catcggcaag gtcgacgagc
     1501 tgtcgtacga gcgagccttt ggctacaccg atccagggca attggtgggc cacctggtga
     1561 tgggagtcga gatgcttggc gagaaggttg agcgctcttc tgagctcact ggagaagcgt
     1621 tccctgccga gctgctcttg cggcttaagc acatgatcgt gagccaccat ggcacgcacg
     1681 agtttggcag tcctaagctg ccgatgacgc tggaagcgat cgcgctgcat tatctcgaca
     1741 acctcgacgc caagatccac accttcagcc gtgagatccg cgacgacccc agccgcgact
     1801 cgagctggac gccgtttcag cagagcctgg ggcgccggct gttcaagggg aacacaccgg
     1861 tagatggagc gggtgatgac ggggcggagc cttgagcccc aggagtggct cgcgatggcc
     1921 gatttcgccg gccgcgtcgc ccagctggtc gcgcggcccg attacaaacc gatcacgctc
     1981 aaggcgatgt cgcgctattt cgacgtgcca gcggacgatt acgccgattt tcgcgcagcg
     2041 gtcaaacggc tcgtgaagga aggtaagctc gacctggcca aagacaagac cctgcgccga
     2101 cccgagcagg cgggcgtgat cgtgggattg ttccgccggt cggccaaggg gtttgggttc
     2161 gtccgccctg ccaattcgac ctcgaagatc gaccatatct acatcccggc cgagtccacg
     2221 cgcgacgctt ccagcggcga cgaagtcgcc gtcaagatca ccaagcgatc gcggcgcgag
     2281 gggatgaacg tcgaaggccg gattgtcgag gtgctcgccc gcgcctcggg gacgttcgtc
     2341 gggacgtata ccgaggtcgg cgaaaccggc tacgtcaaga tcgatggaac gacgtttcgc
     2401 gacccgatct ccgtgggcga tcccggcgcc aaaggggtaa aacccggcga taaggtcgcg
     2461 ctcgaaatcg tgcgatatcc aacacctttt ctcgaagggg aaggcgtcat caccgagatc
     2521 ctcggccagc gcgggcagcc gggagtcgac acgttgacgg taattcgtgc gtttaacatc
     2581 cccgacacgt ttgatgaaga ggtcttggac gaggcgcggg aacagggcaa gcggtttaac
     2641 gaagacgaga tcggcgagcg gcttgatctg cgcggattga cgacggtgac catcgatccg
     2701 gcgacggcac gcgactttga tgatgcgatt tcgctgtcgc gcgacacgca gggacactgg
     2761 acattggcgg ttcacatcgc cgacgtggca cacttcgttc ggcccgggtc agcactcgac
     2821 caggcggccc ggcaccgggg cacgagtgtc tatctgccag atcgtgttat tccgatgctc
     2881 cccgagatct tgtcaaacag cctggcgagc ctacaggcgg ggcgaacccg ctatactgtc
     2941 agcgtgttca tggatttcaa tcctgacgga attctgactt cgaagcgctt tgcccgttcg
     3001 gcgatccgtg ttgaccatcg ttttgcctac gaacacgcac tcgaggtgat gaggaacccc
     3061 caaagcgctc accagggcgt cgcgcccgag attaccggca tgctggcgca aatgctcgag
     3121 ctggcgatga ttctccgccg ccgccggttc gcgcgcggag ggctcgagct ttcgttaccc
     3181 gaggtcgaga tcgagttggg cgaccatggt caggtcaccg gcgcgcatct ggctgtcaat
     3241 gatgagagcc accaggtcat cgaagatttt atgctggcgg ccaatgaggc cgtcgctacc
     3301 agcctgaccg aaaaagacgt gagctttcta aggcgagctc atcccgatcc cgaggctttt
     3361 aagctccttc agtttgcgga ttttgtgcgc agccttggat tgaagctcga ccagcctcag
     3421 agccgtttcg agctgcagcg cgtgttgaaa gaaacgatcg gcaagccaga ggagtacgcc
     3481 gtccattacg gcctcttacg gagcctgaag caggcaaact acacgccgga gcccgagggg
     3541 cattacgcgc ttgctagcct tgattactgc catttcacct caccgatccg ccgctaccct
     3601 gacttgcagg tgcatcggca gttgatcgcc gtgctcgagg ggaaaaagcc gcgcgccaag
     3661 cacgacgaat tggtagtcct cggccagcac tgcaccaaga ccgagcgcag ggccgaggct
     3721 gccgaacgcg agctgatccg gatgaagctg ctttcgttcc tcgaagacca cgtcggcaag
     3781 gcgtttcatg ccgtcatcgt tggagtcgag gattttggac tcttctgccg gctcgtggaa
     3841 ctgccggtcg acggacttat ccatgtcacc agcctggctg atgattttta ctatctcgaa
     3901 tcgggaacgc acaccctggt cggccgccgt tccggccgcc gccaccgatt gggcgatcgt
     3961 gagattgtgc gggtcgcgca cgttgatgtt gaccgccgcg agttggacct ggtcttggct
     4021 gacgatcccg taacgcgggc acgatcggcg aggacgggac ggttggcgac cacgaccgag
     4081 tcacacagcg gcaggcaagg gccagaggtg cgaagacccg gccgcgccga aacaagcgcc
     4141 gccgccgaca ccgagtctgg cccacaacga cgaaaaggcc ccaagaaaaa gaagagcacc
     4201 gccagggcga agaaggccgg aaagaaaaaa aggccctaga gcgaacggtc ttcgttgctc
     4261 tataaagcga tatcctgcgc gccgtcgata agtgtatcgc cgatctgctc gtcccagccc
     4321 ggcgccgcgg gcttggagcc gtcggggcga cgagtcgaaa ttcgactgga aatcgtcagg
     4381 aaaacctggt tcggtctaag ttgcccaggt gggctgccca cggcgcaacc atagcagccc
     4441 ggattgtttc cgcggtcggc agctcaaaga ggagcatttc gccacagtcg agatcgttct
     4501 ccaccaggaa aaaccacctg gaacaacatt cgcacgtacc caaaagctgc gatggctgtt
     4561 ccacgtcggg ttggtgaaga ttgagtgcaa cctggcaatc gggacattgt agtgagttcg
     4621 cccatgcgtt tgagctcacg cttcgagcga ccaattcaaa ggtcaacgga gttgaccgtg
     4681 caatcgcgaa atgggaatgc ttcaacgctt cctccctgtc ttcctcgaca tcaaggaata
     4741 gatggacgga tatctggcaa actttgcctg aggaccacgt cgttctctag ttaggcagaa
     4801 cgctgctata aatatcatag acgagtcatc cattaccgca gtcctaaatt cttgaggaaa
     4861 gacctgactg gttgatagca tgacgcttag cactaagttg aacttaacat acggaagtga
     4921 aaacgccaa
//
LOCUS       DATAQL010000211         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2159846, whole genome shotgun sequence.
ACCESSION   DATAQL010000211 DATAQL010000000
VERSION     DATAQL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159846"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VHS97_03615"
     CDS             <1..1516
                     /locus_tag="VHS97_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIVB peptidase S55"
                     /protein_id="HEX3447311.1"
                     /translation="PFSQMCQYVRSNDRRIAAESKSRAGGKFHAASFGVPLLIDGLAD
                     EAPIRPLSSSQPITVSGGGIGGMTPIVTPLAASGFGPRALNLLSDRLRPLGMAPMSGG
                     AAPERVVREEGDRPLAPGSPLSIAMVMGDFDLSGIGTVTHVEGDRVYGFGHPMFSLGA
                     CEFPMMTGYIHTVYPRASVSMKMGSPLKVVGVIDTDVSTSVAGRIGPKPDMLPLSVRV
                     KTSQFADAHTYRVQMVREPVLLPNLIMAVLTNAIDTEGNLPEELTAQLSATIRIKGFD
                     PISVSDTFSGPRYTGPMGAAALFSPLASMVNILVKNSMAPVRIESIDCDVQIEPGRKV
                     AQIDTVRLVSETIEPGHDLKAIVTLKPFKGERETVELSIPIPADFPEGPCEAVFCDAA
                     NSIRRKFRNDPAVLEPRDVNGVIQTIRIQTEPKRTAVYAHIPSPERGLSVQGQPLPNL
                     PGSVRAVFASKREVPVSPIRTDMVRVAATGWVVEGMHSLRFMVAKDAGLSLSYR"
     gene            1559..>3144
                     /locus_tag="VHS97_03620"
     CDS             1559..>3144
                     /locus_tag="VHS97_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447312.1"
                     /translation="MSHRSYAHGVSPRRPLLWLITFVVACGIMPAVAKVETWRQEGPA
                     AFAKCRREGVVISDSGRVRLGHALSSFGSLAAERVWDLAQTPDGVLYAATGDAGKVFR
                     REAKGDAAWTVAYDSKDTQVLSLAVLPDGTVYAGTGPHGQIVNLSDPKHPASSPDPKV
                     QYIWDLAADAHGNLYAATGPDGQLWKRPADGVWSLSYDSKATHLLCLAIGPGGVVFVG
                     GDGEGLIYRVSPDGKTTILFDAPQSEVRTLLWGPDGALYAGTATEASGANTARGSLFV
                     TQGGGMPRFFDGAGTDRGVDAPSHGGDDGPVDTVRVQAGGLAQARPAAPRPLGGGSAA
                     PKPISAGDNAVYRLDNDGVPREVLRVKALVHALVWADNRLLVGTGPEGQLYEVRDRGE
                     ETAPIAKLDSGQILALLVRPDGTLVIGTGDPGSVVQVAPGYAAAGTLLSEVHDTKLVS
                     RFGSLSWRADLPPRTSIAFETRTGNVGEPDETWSPWSATQSSPASGATVAPLARFIQY
                     RAKLTTADPRRTPDLRSVSL"
BASE COUNT          532 a          977 c         1038 g          597 t
ORIGIN      
        1 ccctttctct cagatgtgtc agtacgtgcg gtcgaacgat cggcggatcg cagccgaatc
       61 gaaaagtcgc gccggtggca aatttcacgc agcgagtttt ggcgtaccgc tcttgattga
      121 cggcctggcg gatgaagcac ctattagacc gctgtcatct tcgcaaccga tcaccgtctc
      181 tgggggtgga atcgggggaa tgactccgat cgtcacgccg ctggcggcat cggggttcgg
      241 tccccgcgct ctgaaccttt taagtgatcg gctccgtccc ctgggcatgg caccgatgtc
      301 gggcggcgcc gcacccgagc gggtggtgcg ggaagaaggg gaccggccgc tggcgccggg
      361 ctctccgctg tcgattgcga tggtcatggg agacttcgat ctctccggca tcggcaccgt
      421 cactcacgtg gaaggcgacc gcgtctacgg gttcggtcat ccgatgttca gcctgggcgc
      481 gtgtgagttt cccatgatga ccggctacat tcacacggtc tatcctcgcg ccagcgtgag
      541 catgaagatg ggctcgccgc tcaaagttgt gggggtgatc gataccgacg tgagcacgag
      601 cgtagccggc cgcatcgggc ccaagcccga tatgctgccc ttatccgtcc gggtcaagac
      661 gagccagttt gccgatgctc acacgtatcg ggtgcagatg gtccgcgagc cggtcctctt
      721 gcccaacctg atcatggcgg tcctcaccaa cgcgatcgac actgagggaa acctgcccga
      781 ggagttgacc gctcagttga gcgccactat ccgcataaaa gggtttgacc ccatttcggt
      841 gtccgacacc ttcagcgggc ctcggtacac tggtccaatg ggggcggcgg ctcttttcag
      901 tccactggcc tcgatggtta atattctggt caaaaactcg atggcgccgg ttcgcattga
      961 atcgatcgac tgcgacgtgc agatcgagcc aggccgcaag gtcgcgcaaa tcgacacggt
     1021 ccgtctcgtt tccgagacga tcgaaccggg ccatgactta aaagcgatcg tcactttgaa
     1081 gccgttcaag ggggagcgcg aaacagtaga actgtcgatt cccatcccag ccgattttcc
     1141 cgagggaccc tgcgaggccg tcttctgtga cgccgccaac agcatccggc ggaagttccg
     1201 caacgatcct gccgtcctcg agccgcgcga cgtgaatggt gtgatccaga cgattcgtat
     1261 ccagaccgag cccaagcgca cagccgtata tgcacacatc ccttcgcccg agcgtggtct
     1321 gtcggtgcag gggcaaccgc tgcccaatct accgggaagc gtgcgggcgg tctttgcgtc
     1381 gaagcgtgag gtgccggtgt cgccgattcg caccgacatg gtacgggtgg cggccaccgg
     1441 ctgggttgtc gaggggatgc actcgctgcg gtttatggtg gccaaggatg ccgggctatc
     1501 gttatcgtat cgttgagatg cttgggcctt gcccgactca acgaccacga ggaacgaaat
     1561 gtcccaccga tcgtacgctc atggggtcag tccccggcgc ccgcttcttt ggttaatcac
     1621 ctttgtcgtc gcgtgcggca taatgcccgc ggtggcgaag gtcgaaacgt ggcggcaaga
     1681 agggccggcg gcgtttgcga agtgccgccg cgaaggtgtg gtcatctcag acagcgggcg
     1741 cgtacggctc ggccatgcac tctcgtcctt cggttcgctg gccgccgagc gcgtttggga
     1801 cctggctcag acgcccgacg gcgtcttgta cgccgcgacc ggcgacgccg gcaaggtctt
     1861 tcgccgcgaa gccaaagggg atgcggcctg gaccgtcgcc tacgattcca aggacactca
     1921 ggtcttgtcg ctggcggtcc ttcccgacgg gaccgtttac gccggcaccg ggccgcatgg
     1981 acagatcgtc aacctgagcg atcccaaaca tcccgcgtcg tctcccgacc cgaaagttca
     2041 gtacatctgg gatctcgcgg ccgacgccca tggaaacttg tacgcggcca ccggtccgga
     2101 cggtcagctc tggaagcggc cggccgatgg cgtgtggtca ctctcttacg atagcaaggc
     2161 gactcacctg ctctgcctgg cgatcggccc gggcggagtg gtctttgtcg gcggcgacgg
     2221 cgagggcttg atatatcggg tgtcgcccga cggcaagaca acaatcctct ttgatgcccc
     2281 gcagtcggag gtgcggacct tgttgtgggg accggacggt gctttgtatg ccgggacggc
     2341 gacggaagcc agcggcgcca acacggcgcg cggaagtttg tttgtgacgc aggggggcgg
     2401 gatgccgcgc ttcttcgatg gggcgggaac cgatcgcggc gtggacgctc cttcgcatgg
     2461 aggcgacgac gggcccgtgg ataccgtgcg cgtccaggca ggggggctcg cccaggcgcg
     2521 gccggcggct ccgaggcctt tgggcggcgg ctcggctgct cccaaaccga tcagcgccgg
     2581 cgacaacgcc gtctatcgtc tcgacaacga cggcgttccg cgcgaggttt tgcgggttaa
     2641 ggcgcttgtg cacgcccttg tctgggccga caatcgcctt ttggtcggca ctgggccgga
     2701 gggccagctc tacgaggtgc gcgaccgcgg cgaagagacc gcgccgatcg ccaagctcga
     2761 tagcggccag atcctggcgt tactggtgcg ccccgatggt acacttgtga tcgggacagg
     2821 cgacccgggc tcagtggttc aggtcgcgcc agggtatgcg gcggccggca ctctcttatc
     2881 cgaggtccac gacaccaagc ttgtgagccg attcggatcg ctctcctggc gcgccgacct
     2941 gccgccgcgc acgtcgatcg cgttcgagac acggaccggc aatgtcggtg aaccggacga
     3001 gacctggtcg ccttggtcgg ccacgcagag cagtccggcg agtggagcga cggttgcacc
     3061 gctggctcga tttattcagt atcgagccaa gctgacgacc gccgacccgc ggcgcacccc
     3121 cgatctgcgg tcggtttcgc tcag
//
LOCUS       DATAQL010000212         7943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_938676, whole genome shotgun sequence.
ACCESSION   DATAQL010000212 DATAQL010000000
VERSION     DATAQL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938676"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VHS97_03625"
     CDS             complement(<1..265)
                     /locus_tag="VHS97_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX3447313.1"
                     /translation="MARRKTLPESVRAKLALAERLAALRSELFGDRGGPEMARRLGIP
                     VRTWYNYEGGVTVPAEVILKIIELTSVEAGWLLHGEEPRFRHAH"
     gene            complement(511..1161)
                     /locus_tag="VHS97_03630"
     CDS             complement(511..1161)
                     /locus_tag="VHS97_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3447314.1"
                     /translation="MNYVLQVVRGRSASTTLKLADGVTVIGRHDDCLIRIKSSQVSRR
                     HCELYEVADKLTLRDLGSSNGTFVNGKKVSGQQALKHGDEVTVGAVTLRVAQLGQAPI
                     SRAEHGSDSKPKAADTAVLEAIAADEDEQEEFEMEFDDGDPEPVVEGIPLADDAETKP
                     TKPKSSPKMATASRTPAPAAEKPADKPSTAKASTEGAKEDDAIAQFLLDLKLDDED"
     gene            1413..1484
                     /locus_tag="VHS97_03635"
     tRNA            1413..1484
                     /locus_tag="VHS97_03635"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1445..1447,aa:Gly,seq:gcc)
     gene            1594..3108
                     /gene="tig"
                     /locus_tag="VHS97_03640"
     CDS             1594..3108
                     /gene="tig"
                     /locus_tag="VHS97_03640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX3447315.1"
                     /translation="MSTNEVESDSSVLVSDASQEPESVAEAPKRKLEIGVDITDVGPC
                     KKHLKISIPRTEIERQYEESLETLRKESVVPGFRAGRAPRQLVVKRFRKQVSDQVKSA
                     LLMTSLEQIDADYKLEPITQPRLDVAAIELPETGPMNFEMDVEVRPQFDVPNYKGLAI
                     QRPVAELTEQHVDEQLTRFLEGHGQIVPKLEGAAVGGDYLTADLTFESSEGVPLSEHK
                     EVEFRLQPEIRFQDGSISDTSPLVGAKPGETRKLEAKLGSVVADPSLRGATATVRVHV
                     IDLKHVRLPESNQAFLDSINVDSIQTLRHAVRETLERRIRTEQRQLIRRQLLDQLLRQ
                     TPFQLPSDLVSREERTTISRLVTQLKREGTSDDVIRAHEAQIRANAHETTLRTLKELL
                     LLSKIADVEGIKVEDQDLVLEIEAMADRTGESVRRIRARVEKEGGADSLATQILEQKV
                     IDRILENSAVQDVEVTIDPEGRVETLDITATVPAVESGSAESDSPTLENIEKGS"
     gene            3236..3874
                     /locus_tag="VHS97_03645"
     CDS             3236..3874
                     /locus_tag="VHS97_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX3447316.1"
                     /translation="MPFEPPLADPMLQRYRDYARQRQMTLGDLLLENRIVFLEGVIND
                     AVANLAVMKFLYLQYENRTQGIHFYINSPGGSVTSTLAIYDTMQFIECPILTYCIGLA
                     ASGAAVLLAGGSKSRRYALPHSKIMIHQPYGQVGGQVSDIEIQAEEIVKSKQVINEIL
                     AKHTGQPIERIARDTERDRYLTAIQAKEYGLVDEVVGRVAGTEKGMGAPPVV"
     gene            3992..4606
                     /locus_tag="VHS97_03650"
     CDS             3992..4606
                     /locus_tag="VHS97_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX3447317.1"
                     /translation="MPLVPIVIERSGREERAMDIYSRLLQDRIIILGSTIDDNVANLV
                     VAQMLVLAHQDAKADIHLYVNSPGGSVTAGLAIYDTMQWVSCDVQTYCIGQCASMGSL
                     LLAAGAKGKRSALPHSRIMIHQPLAGMEGTATEILIHAEEFIRMKKQLNQIYQKHTGQ
                     TLEKLQDDTDRDRFMSPEEAREYGLIDNVVDREPPIPLNPNPIA"
     gene            complement(6376..6801)
                     /locus_tag="VHS97_03655"
     CDS             complement(6376..6801)
                     /locus_tag="VHS97_03655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX3447318.1"
                     /translation="MSSPDQHPIPVGIGIIRRNNRFLIRRRPEGTVYAGYWEFPGGKC
                     EPGEDPAQATARECLEEVGLAIVVGALRRVITYRYPHGLVRLHFYDCTTQEPVAEPPP
                     SSGFQWVMADRLASLRFPEANEAILADLGGGEIYRTTDE"
     gene            complement(6798..7676)
                     /locus_tag="VHS97_03660"
     CDS             complement(6798..7676)
                     /locus_tag="VHS97_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX3447319.1"
                     /translation="MGLFSGKKGMILGVANDFSIAWAIAQKLLAEGAELGFTHLPGDK
                     MERRVRKLADPIGAKLITSCDVQKDDDVARVFDEAKATYGTIDFVLHSIAFAPIDDLK
                     CPFVDASREGFKTAMDISVYSLAIVARHAARVMTDGGSIAAMTYFGGERVVPGYNMMG
                     VCKAALDAAVKYLAYDLGPKNIRVNALSAGPVKTLAASAVGDSDKLAGLYEMTSPLER
                     NITREEVGSAGMLLLSDHARGITGEILHVDCGYNVMGSPGRAIEKAKKLILEKVKAAN
                     PALAAALENDSELKRL"
BASE COUNT         1624 a         2294 c         2437 g         1588 t
ORIGIN      
        1 tgtgggcgtg gcggaaccgc ggttcctccc cgtgcaacaa ccaacccgcc tcgaccgacg
       61 tcagctcgat gattttcaat atgacttcag cggggacggt cactccacct tcgtagttgt
      121 accaggtgcg aaccgggatc cccaaccgac gggccatctc gggcccacct cggtctccaa
      181 acaactctga gcgcaacgca gccagacgct cagctaacgc caacttggca cgaaccgatt
      241 cagggagagt tttacggcga gccatgtgcg gattcctgca tgcagacttg tgtcgacaaa
      301 tcgacccttg gtgccaagcg aatcggcacg attggtcgac gacctagagc tatttaaagc
      361 gttttgccgt agaaatcaat gcgattgtcg acaaagctgc gaaagttcaa caaaaaagtc
      421 gcaaagatcg tgattttgag gtctgacgaa tcgatcgttt cggcaaataa gtcgttatca
      481 gcatttcccg gagatcctgc acggcgcgca ttaatcctcg tcatcgagtt tgaggtcgag
      541 taagaactgc gcgatcgcat cgtcctcctt ggcaccttca gtgcttgcct tggccgtgct
      601 gggtttgtcg gccggtttct cggcggcagg agccggagtg cggctggcgg ttgccatctt
      661 ggggcttgat ttgggcttgg tcggttttgt ttctgcgtcg tcggcgagtg ggattccttc
      721 cactactggt tcggggtcgc cgtcgtcgaa ttccatctcg aattcttcct gctcatcttc
      781 gtccgcggcg atcgcttcga ggacggcggt gtccgcagcc ttgggcttgg aatccgagcc
      841 gtgctcggcc cgggaaattg gggcctggcc gagctgtgcc acacggagcg tcacggctcc
      901 cactgtcacc tcgtcgccgt gcttcaaggc ctgctgccca gagacttttt tgccattcac
      961 gaacgttccg ttcgagctgc ccaggtcgcg aagcgtgagc ttgtcagcga cctcataaag
     1021 ctcgcaatgg cgccggctga cttgcgacga tttgatgcgg atcaggcaat catcgtggcg
     1081 accgatgacc gtgacgccat cggccagctt gagagtcgtt gaggcacttc ggccgcgcac
     1141 gacctggagt acatagttca tcgtggattc gtcccgaaca ctttgcacgt gggttcgcac
     1201 ttcagccgac gaaatgatcg aactttctgt aagttatcgt ttggcgggcg tgtacgcaaa
     1261 tgaaaagccg gcatcaaatt gagggccttc cgggcttttc cgaagacggc ttgcattccc
     1321 ggtccaccgc tctatactga ttttgatgag gctggaaagc ccgaatttcg tttactatgg
     1381 tttgggcgca tctcttgacc tcgctatgag atgcgggagt agctcagggg tagagcacca
     1441 cgttgccaac gtgggtgtcg agggttcaaa tcccttctcc cgcttttcga tagatgccat
     1501 cctgggtcgg cggcttttcg ctcgagcgcg tatggtgcgt gatggcggta gaagccatcc
     1561 gggcgcgata tccaattgag ggaaagcctt tcgatgagca ccaatgaagt tgaatcggat
     1621 tcgtccgttt tggtgagcga cgcgtcgcaa gagccggagt ctgttgcgga ggctcccaag
     1681 cgcaagcttg aaatcggcgt cgacatcacg gacgttgggc cgtgcaagaa gcatctcaag
     1741 atctcgattc cgcgaacaga gatcgagcgg cagtacgagg agtcgctcga aactctccgt
     1801 aaggaatccg tagtgcccgg cttccgtgcg gggcgcgctc ctcggcaact cgtcgtcaag
     1861 cgtttccgca agcaggtttc cgaccaggtc aagtcggccc tgttgatgac gtcgctcgag
     1921 cagatcgacg cggattacaa gcttgagccg atcactcagc cccggctcga cgtcgcggca
     1981 atcgagcttc ccgaaaccgg accgatgaac tttgagatgg acgtcgaagt tcgtccgcag
     2041 tttgacgtgc cgaattataa gggcctcgcg atccaacgtc cggtcgcaga gctcactgaa
     2101 cagcacgtcg acgagcagct cacgcgcttt ctcgagggcc atggccagat cgtccccaaa
     2161 ctcgaagggg ccgccgtagg tggcgactat ctgacggcgg acctgacgtt tgaaagctcg
     2221 gagggcgtgc ctctgagcga gcacaaggag gtggagtttc ggctgcagcc cgagattcgt
     2281 ttccaggatg gctcgatttc cgatacgagt ccacttgtgg gcgccaagcc cggcgaaacg
     2341 cgcaagctcg aagccaagct gggttcggtg gtggccgatc cgagtttacg cggagcgact
     2401 gcaacggttc gcgtgcatgt catcgatcta aagcacgtga ggcttcccga gtcgaaccag
     2461 gcatttctcg actcgatcaa cgtcgacagc atccagacgc tgcgtcacgc cgtgcgcgag
     2521 accctggagc gtcgaatccg gaccgaacaa cggcagttga ttcgccgcca gctcctcgat
     2581 caacttttgc ggcaaacacc gtttcaactc ccatcggacc tggtgtcgcg ggaggaacgg
     2641 accacgatca gccggctggt gacgcaactg aaacgagaag ggacgtcgga cgacgtcatt
     2701 cgcgctcacg aagctcagat tcgcgccaat gctcatgaga cgacgctgcg caccctcaag
     2761 gaactgctgc tgctttctaa aattgccgac gtcgaaggga tcaaggtcga ggaccaggat
     2821 cttgtcctcg agattgaggc gatggccgat cgtacgggag agagtgtgcg gcggatccgc
     2881 gcgcgggtag aaaaggaagg tggggccgac tctttggcga cgcagatcct cgagcagaag
     2941 gttatcgacc ggatactaga aaacagtgcg gtccaagacg tcgaggtcac gatcgatccg
     3001 gagggaagag tcgaaaccct cgacatcacc gccacggtgc ccgctgtcga atcgggatcc
     3061 gcggaaagcg atagcccgac gttggaaaat attgaaaaag gatcgtgaac cctctcgcgc
     3121 gacgaggctg gtggaaatgc cccgttgacg ttccacgtgc cctggctctt tggcttgtgc
     3181 tcaatttcga gcatgggccc atgcggaccg ggccctgatt ccgaaggacc gttccatgcc
     3241 gtttgaacct ccgctcgccg atccgatgct tcagcgctat cgcgactatg cgcgccagcg
     3301 gcagatgacc ttgggcgatc tgctcctcga gaaccgcatc gtcttcctcg aaggagtaat
     3361 caacgacgcc gtggccaacc tcgccgtcat gaagttcctc tacctgcaat acgagaatcg
     3421 tacgcagggg atccactttt atatcaacag cccgggtggt agcgtcacca gcacgctcgc
     3481 gatctacgac accatgcaat tcattgagtg cccgattttg acctattgca tcggtctggc
     3541 ggcctcgggc gccgccgtgt tgctcgccgg cggatccaag tcccggcgtt atgcactgcc
     3601 tcactcgaaa atcatgatcc atcagccgta tgggcaggtg ggaggccagg tctcggatat
     3661 cgagatccag gccgaagaga tcgtcaagag caagcaagtg atcaacgaga tccttgccaa
     3721 gcacaccggg cagccgatcg agcgcattgc gagggacacc gagcgcgatc gctatttgac
     3781 cgccattcag gccaaggaat acggcctcgt cgacgaggtt gtgggtcgag ttgccggcac
     3841 ggaaaagggc atgggcgcgc cgccggtcgt ttgagcagag acgaaatcac cgagctgacg
     3901 attttccgcg agctgctccc ggtcgcttga gcgctaagtt ttctaaatgg cggccgccgg
     3961 ccgacgattc ctcttcgaga agggctatat catgccactg gttccaatcg tgatcgaacg
     4021 cagcggtcgt gaagaacggg ccatggatat ctattcccgc ctgttgcaag accgcatcat
     4081 catcttgggc tcgacgattg atgacaatgt cgccaatttg gtcgtggctc agatgctggt
     4141 cctggctcac caagatgcca aggccgatat tcatctctac gtcaatagcc ccggtgggag
     4201 cgtgactgct gggctcgcga tttacgacac gatgcaatgg gtctcgtgcg acgtgcaaac
     4261 ctactgcatc ggccagtgtg ccagcatggg ttcattgcta ctggcggcgg gggccaaggg
     4321 aaaacgcagt gcgctgccgc acagccggat catgattcac cagccgctgg ctgggatgga
     4381 ggggactgca accgagatct tgattcacgc cgaagaattc attcggatga agaagcagct
     4441 caatcagatc tatcagaagc acacaggaca gacgctggaa aagctgcagg atgataccga
     4501 ccgcgatcgg ttcatgtccc cggaggaagc gcgggaatat gggctgatcg ataacgttgt
     4561 cgatcgcgag ccgccaatcc cgctgaaccc gaatccgatt gcttagttcc gcgccaggga
     4621 gagaaggccc ggaatcccgc cgggagggcg agcctccgtg cgagccggca cgtggaaaag
     4681 gctcgaacgg gcgctgctcc ggtcgtggcg gcgggatcgc tcgggctacc gagcgtgtgg
     4741 gcgattggcg tcgccccatc gtaacggcgt cggggggcgg cggacagggg gccgccgcgc
     4801 ggggagcacg gctgccgacc aggggcatcg ctatctggag agatcctccc aaccgatcgc
     4861 cacggggacg atcggtctcg gccgaatccg cggatcggtc acgatcgaaa aaccgcaccg
     4921 gcgcgctcgg acgcagaacg aggagcgctc cgggagatag tgaggctccc cctccacgtg
     4981 ggcgatcagc ggcaagaaga agggaaagaa cgggaaaccg gtgcgcctcc gacacgggca
     5041 gaggtcgcct tgctgctgga tggccagata gacctcgcgc ccgctgagca ggaacctcgc
     5101 ccccgggccg atcaggaagg ttcacggtag cgattggcac ggactcgtcg tcgccgtgct
     5161 cactgaaccg atccattggc tccgggcccg gcgccgtaaa acaacgaggg tgaggatcgc
     5221 gatcgccgcg ctcgccagga tcggggaccg catctcgagg gcagaccgca cccggtctcg
     5281 gatcacgaag ggctgcgacg gtagcggccc tggagtccat tcgaacagga ccggaaacca
     5341 tcgttgccat agagcccgcg atggaggaat ggagccgtga gggatcgact ttggatctga
     5401 agggaattca tcatagccga ggatactgcc atcctccttg aacacgactg ttttgatcgg
     5461 tcgaaggtcg acctcgctac gtattaggaa cgggatggaa ataacttccc gttttttcgc
     5521 caagctcccc cgatcagcca gtcctgaggc aacgcgaagt cgggaggttt tttccgtccc
     5581 gttctttggt acgtacaaat ccggtttggc gatgtccccg cgcgagttgt cctgacggag
     5641 aacgggacct cgcatggccc catgcgagtt gtgccgacgg agaatgggac cttgaacgaa
     5701 cccatttgcg agcacgtaat agacgtcgcc ctcgttatcg atggtgacat cagagagatc
     5761 gatcaattta ctccttatct cctcatcaga gggataaggt ggtggacgat aggccgcgcc
     5821 ggcgatcatc aatgcgatca aggcgcaacc gatcgccatc catcgcacca tggaacgaaa
     5881 cgggtgctgc cgagctgaag tctcctgact gcgtgatgaa gatgggcgct ggccgcgtcg
     5941 cgcgggggat ccgatcagca cggtgattac caggatcgtg atcgcaacag aggcaaggac
     6001 gggcgcccag atctgtggcg tggtcggcgg taggcgacga tacaagatgc gcaccatgaa
     6061 cctctggctg gcgctgatgc tgaaatcagt ccgtacgacg tatatcgaac catcacttag
     6121 aaaaaaggaa tgtcctctcc cgaaatcaga acctcctccc tccgaactcg ggtcgatcgg
     6181 gcgaaaactg cgtgtggcga cggcacctgt taagttcagg ccgatcaggg cgatcacgat
     6241 gaggagtaac cggatcgata aacgaagagc aggataaggc atggctcgag tcccggatca
     6301 gaccgcggat caggctccag gcggctcgcc ggccacatag ggactggtca gagggttcgc
     6361 cgtcgagttc acccattatt cgtccgtggt tctatagatc tctcctccac caagatcggc
     6421 gagaatcgcc tcgttggctt ctgggaagcg cagcgacgcg aggcgatcgg ccattaccca
     6481 ttgaaagccc gagcttggtg gaggctcggc gacaggttct tgggtcgtgc aatcatagaa
     6541 gtggagcctg accaagccgt gcggatagcg gtacgttatg acgcggcgca gggcgccaac
     6601 cacgatcgcc agtccgactt cctcgaggca ttcgcgggct gtcgcttggg ccgggtcttc
     6661 gcccggttcg catttccccc cggggaactc ccagtagcca gcgtagaccg ttccctccgg
     6721 ccgtcgccgg atcaggaagc gattattccg gcggataatg ccgatgccta ccgggatcgg
     6781 gtgctggtcg ggcgagctca tagcctcttg agctcgctgt cgttctcgag cgcggccgcc
     6841 agagcagggt tcgcggcctt gactttttcg aggatcagct ttttggcctt ctcgatcgcg
     6901 cggcccggag agcccatgac attgtagccg caatcgacgt ggagaatttc gccggtgatc
     6961 ccgcgcgcgt gatcagagag caataacatt cccgccgagc caacttcctc gcgggtgatg
     7021 ttgcgctcga gcggcgaggt catctcgtaa agtccggcca gtttgtcgga gtctcccacc
     7081 gccgacgcgg ccagcgtttt gactggtccc gccgacagcg cgttgactcg gatgtttttc
     7141 ggccccaggt catacgccaa gtatttgaca gcggcatcga gcgcggcctt gcagacgccc
     7201 atcatattgt agccggggac gacccgctcg ccgccgaagt aggtcatggc cgcaatggat
     7261 cctccgtcgg tcatcacgcg agccgcgtgt cgagccacga tcgccagcga gtagacgctg
     7321 atatccatgg ctgtcttgaa tccctcgcgg ctggcgtcga caaaggggca tttcaagtcg
     7381 tcgatagggg caaacgcgat cgagtggagc acgaagtcga tcgtgccgta ggtcgctttt
     7441 gcctcgtcga atacgcgtgc gacgtcatcg tctttctgga catcgcacga agtgatcagt
     7501 ttggcgccga tcgggtcggc caatttgcgg acgcgccgtt ccatcttgtc gccgggcagg
     7561 tgggtgaaac cgagctctgc tccctccgcg aggagtttct gcgcgatcgc ccaagcgatg
     7621 ctgaagtcat tcgccacacc caagatcatt ccttttttgc ccgaaaacag tcccatcccc
     7681 ggcccctttc gcctgtacgg cgccaaaatg tcttctggct catccgcgtt tggtcgtcag
     7741 cgttgtaaaa taccgggact caccggcccg ctcaacgtcg actttgcgcc gaggctggac
     7801 gagcacggcg aaggggtatt ttcatgcagc cagtatggac cggacggaag gcggacgccg
     7861 gcgacaagtc agaagtgacg acgcccgtgc gaaaatggtt tacggccgaa ctctggacct
     7921 ggctgcttgt cggggtcggc gtg
//
LOCUS       DATAQL010000213         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1396670, whole genome shotgun sequence.
ACCESSION   DATAQL010000213 DATAQL010000000
VERSION     DATAQL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396670"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..1727
                     /gene="mgtE"
                     /locus_tag="VHS97_03665"
     CDS             348..1727
                     /gene="mgtE"
                     /locus_tag="VHS97_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX3447320.1"
                     /translation="MLGKLALPEIRELIEAHDDDTLHEVVNRWHSADLADLVNALPIS
                     EQVHVLRLIKPPLAAVAFAYLDLDAQQDVLTSLSEEESKYILNEMAPDDRTALLAELP
                     PDQAERLIDLLDPDQRAIARALLNYGEDSVGRLMTPDYVVVRKEWTMRHVLDHIRAHG
                     RDSETLNVIYIVDDQNKLIDDLRIREILLAPLHTHVADVMDNKFVSLAATDDKKKAVE
                     VFRKYDRIALPVVDSQGRLAGIVTLDDVIDVAEEEATREIQRFGGLEALDDPYMSTTL
                     PAMVRKRATWLVILFVGEMLTATAMGFFEKQIERAVVLALFVPLIISSGGNSGSQAAT
                     LIIRALALGEVRLGDWWRVMRRELVAGLSLGLILASIGFVRIAAWNAVSNLYGPHWLL
                     VGLTVSLSLIGVVLWGTLTGSMLPFLLRWMGFDPATSSAPFVATLVDVTGLIIYFTVA
                     IMLLSGTLL"
     gene            2072..2671
                     /locus_tag="VHS97_03670"
     CDS             2072..2671
                     /locus_tag="VHS97_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447321.1"
                     /translation="MPAKEGLALAPPLFEASRIAQSISSPARLSSVTRPAIVDEPDSS
                     PRAVDPDDSQYELPVSTIVDIEDSQVELPAFTADRSTSDDNWPTANDDSPSGELTLSS
                     PWYLSFVDSWGRYHFAVAIGFGAFSLALLGFFLTREILGGQTIGATVTLLIVGCVVMV
                     AFLLLSVTVTALNLLLADLTKNVRQLRIQSEPKTRIVSD"
     gene            2878..3204
                     /locus_tag="VHS97_03675"
     CDS             2878..3204
                     /locus_tag="VHS97_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEX3447322.1"
                     /translation="MDEFEGFPEDVRNHLSEQGSDLTLPHSLDLYLYYPSEIEAQAVA
                     AHFLESPLESDVIESDQHWLCLVQCRLVPTQDGLEAIAELMRTAHDQFGGDFDGWHAE
                     IVPATE"
BASE COUNT          747 a         1126 c         1065 g          807 t
ORIGIN      
        1 ggatatcagg gtccagggaa gacatgcgat gctaatgcgg cgatctggcg cggtcaatgg
       61 ccctgaatcg catacgcgcc caaaatcatt cggttcatgg atccggattg tcgtgctccc
      121 acaaagctcg atccacgaac catctctatc gactaccgat ctctgatgac ataatcgact
      181 gatcagcgac gtcgagccgc cgggtgaata ttgcccaggt gttttcgcac tgggacacga
      241 agccttgtcc cacgagccat cggccatagc gcgacacgcg atgtgatcga ccggcccgag
      301 cggccgtgat ttggcctctt gatcgcccga ttgagaaata aactgcaatg ctgggaaagc
      361 tggcgctacc ggaaatccgt gagctaatcg aagcccatga cgacgatacc ctgcacgagg
      421 tcgtcaaccg ctggcactcc gccgacctcg ccgaccttgt caacgcactg cccatcagcg
      481 agcaagtgca cgtcctgcgg ctgatcaagc ccccactggc cgccgtggct ttcgcttacc
      541 tcgatctcga cgctcagcag gatgtcctga cgtcgctctc cgaggaagag tccaagtaca
      601 tcctgaatga gatggcgccc gacgaccgga cggcgctcct ggccgagttg ccccccgatc
      661 aggccgagcg actgatcgat ctgctcgatc ccgaccaacg agccattgcc cgcgccctgc
      721 tcaactacgg cgaagacagc gtgggccggc tgatgacgcc cgactatgtg gtcgtccgca
      781 aggaatggac catgcggcac gtgctcgacc acatccgcgc tcatggccgc gatagcgaga
      841 cgcttaacgt gatttacatc gtcgacgacc agaataaact gatcgacgac ctgcgaatcc
      901 gggagatact gctcgctcca ctgcatactc acgtcgccga cgttatggac aacaagttcg
      961 tcagtctggc cgcgaccgac gacaagaaga aggccgtcga agtcttccgt aagtacgacc
     1021 ggatcgcgct gccggtcgtc gattcgcagg gccgcctggc ggggattgtg actctcgacg
     1081 acgtgatcga tgtcgccgag gaagaggcaa ctcgcgagat tcaaagattc ggcggtttgg
     1141 aggcactcga tgatccttac atgagcacga cgctgccagc catggtgcgc aagcgggcaa
     1201 cctggctggt gatcttgttc gtgggcgaaa tgctgacggc aacggcaatg ggtttttttg
     1261 aaaagcagat tgaacgcgcg gttgtcctgg ccctcttcgt gccgctcatc atttcgagcg
     1321 gcggaaattc gggctcgcag gcagccactc tcatcattcg cgcactggca ctgggcgaag
     1381 tccgtctcgg cgactggtgg cgcgttatgc gccgggaact cgtcgcgggt ttgtcgctgg
     1441 gtttgatact ggcctcgatc ggcttcgtcc ggatcgccgc ctggaatgcc gtgtccaacc
     1501 tctatggtcc ccactggctc ctcgtcggct tgacggtgag cctctcgttg atcggcgtcg
     1561 tgctctgggg cacattgaca ggatcgatgc tcccctttct gctgcgatgg atggggtttg
     1621 accccgccac atcctcggcc ccattcgtcg caaccctggt tgatgtcacc ggactgatca
     1681 tctacttcac cgtcgccatc atgctcttga gcggtacgtt gctctaagcg cgccgccatc
     1741 cccgagtcaa accaaagtcg acggccgatc ggcacgtggc gcgaaatatg ccctcattaa
     1801 gcacgaccga tcgatttgcg cgtttgcaca ccaagttgaa cggagttgaa aacaatcaac
     1861 gagtatgggg acttcgttga atttggttca cttgctgcct ataaaggtgc tgaacgtccg
     1921 cggcaggatc cgtcttggcc gtaaggtaaa ttcgcccatg atgatccgat gtccaaattg
     1981 tgaacgaatc ggcaatttag cagttcatct tggattgaat gcgcgcgttg tccgttgtcg
     2041 ccgctgcggc acccgattca cgatcgttcc cttgcccgcc aaagaaggac tcgcgctcgc
     2101 tccaccgcta tttgaagcgt ctcggattgc tcaaagtatc agcagtcccg cccgattatc
     2161 gtccgttaca cggcctgcaa tcgtcgacga accggattcg tcaccacgag ccgtcgaccc
     2221 cgatgattct caatacgaat tgccggtctc tacgatcgtc gacatcgaag actcgcaggt
     2281 cgagttgccc gcatttactg ccgatcgttc aacttcggac gacaattggc ctaccgcgaa
     2341 cgacgattct cccagcggag aactgacgct ctcgagccct tggtacctca gtttcgtgga
     2401 ctcttggggc cgctaccatt tcgccgtggc aatcgggttc ggtgcattct cgctcgcgtt
     2461 gcttggcttc tttctgacaa gagagatact tggcggccaa acgatcggcg cgaccgtcac
     2521 tttgctgatc gtaggctgcg tcgtcatggt tgctttcttg ctactctcgg tgaccgtgac
     2581 cgctctcaac ctgctgctgg ccgatctgac caagaacgtc cggcaattgc ggatccaatc
     2641 ggagccgaag acgcggatcg tcagtgactg atggaaaaac agtcggttgc attgacctgg
     2701 tttgtgggtc gccgcccgtg tgagcgctga cgtcgatccg atgctcccca gcaattttgg
     2761 ttgcaactcg ccgtcgcgag cgggacaatc cagtggttgg agaacgtcgc ctttccgctg
     2821 tgcatcccgg cgttttgcgc cctaaccgcg tgaagaatcg ccagatagga aatcaggatg
     2881 gacgaattcg agggtttccc ggaggacgta cgaaaccatc tctccgaaca gggctcggat
     2941 ctgacgcttc ctcacagcct ggacttgtat ctctactatc cgtcggaaat cgaggcccaa
     3001 gcggtggcgg cgcactttct tgaatctccg ctcgagtccg atgtgatcga gtcggaccaa
     3061 cattggctct gcctggttca atgccggcta gtcccgactc aagacgggct ggaagcgatc
     3121 gccgagttga tgcggacagc gcacgaccaa tttggaggcg actttgacgg atggcacgcc
     3181 gagatcgtgc ccgcgaccga atgaaggaaa aatgcaggcg gccgataggc atcgcgtttg
     3241 tcggccgctt cgttaggggc cgagtcatcc tcgcctgacg gcgactaaaa tcgatccgaa
     3301 ctgaccactt cgccttgctc ccgctgcctt gaagtcgccc gcaatcggcg gtctacaccc
     3361 ctggcggcgg ttgcgggtag agagaacccg tcaatagacg gcaggaattg atgccgtgac
     3421 gctgcgccga ggtctgactt ttcgaccacg gctccaatcg acatcgcctc gcagttttcg
     3481 gtgttcctcg aagcgtggtc ggaacactcg tcgcttgtcc ccgccaggca gaaaatgggg
     3541 caaagcagcg ttaacgcaaa acagatgatc actccaacgc ggattattag atcgtcttac
     3601 cgttcagagg tcgttctgtc attggaggtc cggcgtgagt tggtcgtcgc ggcttggctc
     3661 ggggtcattg accggcggtt gcgagcatgg ttcaaccaca cctctaccac ggaacctccg
     3721 agcacgcgcg ccgcgctgcc aatct
//
LOCUS       DATAQL010000214         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_633442, whole genome shotgun sequence.
ACCESSION   DATAQL010000214 DATAQL010000000
VERSION     DATAQL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633442"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(508..2799)
                     /locus_tag="VHS97_03680"
     CDS             complement(508..2799)
                     /locus_tag="VHS97_03680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3447323.1"
                     /translation="MNRWNLRQSLRESGPEEAGDANAPRFTSSSETFFRDAVAEWNGT
                     GHGRIELDSHSASVTRLARVATGHRPIRDANVPVRDSESRASEQLGTTAPRVGDQLAG
                     FRILARLGRGAQGRVYLATQTELADRPVVLKVSPLRGGEHRSLARLQHTNIVPILSSQ
                     DLPEQGLRVLCLPYMGGTTLEHVLLKLMLRPVGERSGRQLLEVLDQTTNLALVSLPAA
                     GPAREFLAVESYERSIIWIGLCLAEALHHAHLRNLVHCDVKPANILLAADGTPLLLDF
                     HLAQPPLDFGAAPPLWIGGTPPFIAPEQAIALAAMTEGRRAPSVGARADIYALAMVLH
                     IALTDELPSGERRIVSGMRRANPRISPGLAAIIARALAKDPQERYPSAAIFAEDLRRH
                     LDDRPLVGVSNHSLMERWTKWRMHHPIAVARAGADVSAVLGLVTAGFIAWFAADRRLN
                     DAIGELEQGRIHVAAGDHRQAAQHLDHGLMLLESGTPLDRLLPRAREARDSIIATRDQ
                     NRRAEMAVQLHDLADRVRFLFAASPTRTAAARKLNDRLAEMWHARDRIDLQLAKEADE
                     GTRARVRADLLDLSILWAELRVELADEVTRPEAHREAILTLAQAEADWGPSPVLSQEQ
                     SFHAEAIGLAEVSAPRPSAMGPRTAWECYALGRAMLRHGDLEPAADLLDRAVALQPRS
                     LWAQFYRGRCALLRNRYAEAVDAFGACVALAPEAVCYYNRSLAYSGLGAKTRARLDLE
                     RARELDPTLVASPQGPRRDPVRR"
     gene            complement(2811..>3241)
                     /locus_tag="VHS97_03685"
     CDS             complement(2811..>3241)
                     /locus_tag="VHS97_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447324.1"
                     /translation="ASDPVTTSSLIGVGDDGSAGRGRLQDELVVTRASSADPQVPTGR
                     GTAPAELVPVNLESLERALGQLMRQIDEIGGDVAQWFTRVGALEMLLAAGMVGMACEL
                     IRRCERRSQLMNAHTRLGRSSLPGPFYRRKACPFAGTSIR"
BASE COUNT          587 a         1034 c         1097 g          523 t
ORIGIN      
        1 cggcccgtat ctgggactcc ggcccaccca cggacgaggt cattcgcgat cgctgacgcg
       61 caggcgagcg cagcgaggtc atttggggtc cactcgggtc gtcccctcca ttgtctgagg
      121 acggccgact ggccgaaacg atgaaatgtc caaggtaaga gacgtgacag agtcaaaggg
      181 cacgcatcgc ggctcgggtt gcattgcatg tcctggagtc ctgtcctgga gtccgtgtca
      241 tggagtccgc aagttgtccc gggggcgagt gccacgaatg agcgtccgaa ttcgaccgta
      301 ggaagtgcag cggaacgagc gccggctgcc aacgcggatg accggtgact tcgcccacca
      361 tctggtcaag ccgaaatcgc tgggacagat gtggccacaa aaacgcgaac gagcgaatgc
      421 aggaaaaact aagccacaag cttagccaag gcaggctgca acaccctcga caatcgaact
      481 gcgagaggct ttgggatgga ttgcatctca acgccggacg ggatcgcgcc tcgggccctg
      541 cggcgaggcc acgagggtcg gatcgagctc gcgggcccgc tcgaggtcga gccgtgcgcg
      601 ggttttggcg ccaaggccgg agtaggcgag agagcggttg taatagcaga cggcttcagg
      661 ggccaaggca acgcaagcgc caaatgcgtc gacggcctct gcgtagcgat tgcggaggag
      721 ggcgcaccgg cctcgataaa actgggccca taggctgcgg ggttgcaggg cgacggcgcg
      781 gtcgagcaga tcagcggccg gttcaaggtc gccgtggcgg agcatggcgc gcccaagggc
      841 gtaacactcc catgcggtgc gcgggcccat ggcgctgggt cgtggcgccg aaacctcggc
      901 caagccgatg gcctcggcgt gaaagctctg ctcctgcgat aagaccggag acgggcccca
      961 atctgcctcg gcctgggcca acgtgaggat cgcttcgcga tgggcctccg gtcgcgtcac
     1021 ctcgtcggca agctcgacgc gaagttccgc ccacaggatc gacaggtcga gtaaatcggc
     1081 gcggacgcgc gctctcgtcc cctcgtccgc ctccttcgcc agctgcaggt cgatccggtc
     1141 gcgtgcatgc cacatctccg cgaggcgatc attgagcttt cgggcggcgg cggttctcgt
     1201 cggactggcc gcaaagagga agcggacgcg atcggcaaga tcgtggagtt ggacggccat
     1261 ctcggcccgc cggttctggt cccttgtcgc gatgatcgaa tctcgcgcct cccgggcgcg
     1321 gggcaaaagg cgatcaagag gcgtgccgga ttcaagcagc atcagcccgt gatcgaggtg
     1381 ttgggcggcc tgacgatggt cgcccgctgc gacatgaatc cgaccctgct cgagttcgcc
     1441 tatcgcatcg ttgaggcggc ggtctgccgc gaaccaggcg atgaaacccg ccgtgactaa
     1501 gcctagcacg gcgctgacgt ccgccccggc ccgagccacg gcgatgggat ggtgcatgcg
     1561 ccacttggtc caacgttcca tcaggctatg gtttgagacg cccaccaagg gccggtcgtc
     1621 gagatgcctc cttaggtctt ccgcgaagat ggcggcgctc ggatagcgct cttggggatc
     1681 cttcgccagg gcgcgagcga tgatcgcggc cagcccgggg ctgattcgag gattcgcccg
     1741 ccgcattcct gagacgatcc tgcgttcacc cgacggcagc tcgtcggtga gcgcgatgtg
     1801 caacaccatc gccagcgcat agatgtctgc tcgagcgccg acggacgggg cacgtcggcc
     1861 ctccgtcatc gccgcaagcg cgatggcctg ctcgggcgcg atgaacgggg gagtgccgcc
     1921 gatccacagt ggcggcgcag cgccgaaatc cagagggggc tgggccaagt gaaagtcgag
     1981 caagaggggc gtgccgtccg ccgcaagcaa gatgtttgcc ggcttcacat cacagtgaac
     2041 gagattcctg agatgcgcgt ggtgaagcgc ctcggccagg cacagcccga tccaaatgat
     2101 cgaccgttcg taggactcca cggccaggaa ctcacgagcg ggacctgcgg ccggcagcga
     2161 gaccagggcg aggttcgtgg tctgatcgag cacctccagc agttggcgtc cggagcgctc
     2221 tcccacgggg cggagcatca gctttaaaag cacgtgctcc aatgtcgtcc cgcccatata
     2281 gggaaggcag aggaccctca ggccctgttc gggcagatct tgcgaagaca ggatcggcac
     2341 gatattggtg tgctggagcc gcgccaacga ccgatgctcg ccgccgcgca gcgggctgac
     2401 cttgagtacg accggtctgt cggccagctc ggtctgcgtc gccaggtaga cccgcccctg
     2461 ggcccctcgt cccagccggg ccaatatgcg aaaaccggca agctggtccc ctacgcgggg
     2521 ggcggtcgtt ccgagctgct ctgaggcccg agattcgctg tcacggaccg gcacattcgc
     2581 gtcgcgaatg ggtcgatggc cggtcgccac tcgcgcgagc ctggtgacgg aggctgagtg
     2641 gctgtcgagc tcgatccgcc catgaccggt cccgttccac tcggcgacgg cgtccctgaa
     2701 aaatgtttcg gacgaagacg tgaaccgcgg cgcattcgca tccccagcct cctcggggcc
     2761 gctctcgcgg agactttgac gaaggttcca tcgattcatg gagcaaccag tcaacggatc
     2821 gacgtcccag cgaacggaca cgccttccgg cgataaaacg ggccgggcag gctcgaacgt
     2881 ccgagtctgg tgtgggcgtt catgagttgg ctccggcgtt cacaccggcg gatgagctcg
     2941 caggccatgc ccaccatgcc cgcggccaag agcatctcga gtgcgccgac tcgcgtgaac
     3001 cactgggcca catctccgcc gatctcgtcg atctgccgca tcagttgccc gagggcacgc
     3061 tcgaggctct ccaggttaac gggcaccagt tccgcgggcg cggtgccgcg gcccgtcggc
     3121 acttgcgggt ccgcggagga cgctctggtg acgaccagtt catcctgaag acgaccccgg
     3181 cccgcggatc catcgtcccc gacgccgatc aggctggaag tcgtcaccgg gtcggacgcg
     3241 t
//
LOCUS       DATAQL010000215         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2465294, whole genome shotgun sequence.
ACCESSION   DATAQL010000215 DATAQL010000000
VERSION     DATAQL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465294"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..359)
                     /locus_tag="VHS97_03690"
     CDS             complement(93..359)
                     /locus_tag="VHS97_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447325.1"
                     /translation="MTDLEQEAAWRAEFEADGERAVLDTICHGTGIYPDPKRLFAVRW
                     LRERRVERACREQKMNSYVRYTFWSAVVAVVTGIVGVLVTWFSR"
     gene            962..>1744
                     /locus_tag="VHS97_03695"
     CDS             962..>1744
                     /locus_tag="VHS97_03695"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3447326.1"
                     /translation="MARTGRPQDRLLSLSAIEREELEHLGRSRSAPHSLVRRAQIILG
                     SASGETNLSIARRLRVSNPTVCHWRRKWFDQGLVGLYGEARPGRPRTHDEERVAELLR
                     TVLDSKPANATHWTVRSAAATTGLSKSTVARTFALFGVQPHRSKHFKLSTDPEFVDKV
                     REIVGLYLNPPDHALVLCVDEKTQIQALERSQPILPLGLGYIEGVTHDYYRHGTTTLF
                     AALDIANGQVIAQCRARHRHQEFLSFLKHIEANVPRDLDLHLV"
BASE COUNT          355 a          536 c          519 g          334 t
ORIGIN      
        1 agcggcccaa ctggcggtgg ctgattgaat agctcttgct tgtcgcctcg caccagaacg
       61 tcatcaaatt cgagaaacat gctcggccat gcctagcgag aaaaccaagt caccagcaca
      121 ccaacaatcc ctgtgaccac tgcaacaacc gctgaccaaa atgtgtagcg gacgtaggag
      181 ttcatcttct gttctcggca cgctcgctca actcgtcttt cgcggagcca acgcacggcg
      241 aacaaccgct ttgggtcagg atagatgcca gtgccatgac agatcgtgtc gagcacagca
      301 cgttccccgt cggcctcaaa ctcggcgcgc cacgcggcct cttgctccaa gtctgtcatg
      361 gtgcttcgcg gaggtttcaa cggtacccca actcgatgac gacaccctac cacagcaatg
      421 gcagtcgaac tggagtgttc gcagcttttt cacggacagc atcctcggcc gctaggctcg
      481 gcaggctacg aggcggaacg cacagcgagc ggcgaggttt tgggctgagg atcaagggcg
      541 tcaaccggct ggtcagggcg atggacgcgg aaatcatcag gcccgccgag gcgcggtttg
      601 acgagcttgt gatgcggaga gctgagacgt tgagggtcag gcgcgtgtga gcgaagccag
      661 ttgccgggcg gcgggattgg tgttgagacg ctcatcgccg cgaaactcag aaaggctgat
      721 gagcggcatg tcgctccccc ggagcctcgc ccatggtggc gtcgcgtcgg agagatttcg
      781 gcttgcacgc cccagaccgc cgaggccctc ttatgcgatt gctaatcggc aggggcggct
      841 tgtgggagga cactcgaaca tgccttggct gccctgcagc gggagctagt ctcatgtccc
      901 ataaatatct ttacataatc tgcgaggatc gccatgattc gtttttgctt tggagcggat
      961 cgtggcgcgc actggaagac cgcaggatcg tttgttgtcg ctgagcgcca tagagcggga
     1021 agaactcgag cacttgggtc gatcgcgttc ggcccctcac agcctggttc gtcgagccca
     1081 gatcattctg gggtcggctt caggcgagac caatctcagc atagcccgac gactgcgagt
     1141 ctcgaacccg accgtgtgcc actggcgcag gaaatggttc gatcagggcc ttgtcggcct
     1201 gtacggcgag gcgaggcccg ggcgcccgag aacccacgac gaagagcgag tggcggagct
     1261 tcttcgcacc gtcctcgaca gcaagcccgc caacgcgacg cactggactg tgcgctccgc
     1321 cgccgcaacg accggccttt cgaaaagcac ggtcgcgcga accttcgctt tgttcggcgt
     1381 acagcctcac cgctccaaac atttcaagct gtcgaccgat ccggagttcg ttgacaaggt
     1441 ccgggaaatt gttggtctgt atctgaatcc accagatcac gccctcgttc tctgcgtcga
     1501 cgaaaagacg cagatccagg cgcttgaacg tagccaaccg atcctgcctc tggggctcgg
     1561 ctacatcgaa ggggtgaccc acgactacta tcggcatgga acgacgacgc tgttcgccgc
     1621 tctcgatatc gccaacggtc aggtcatcgc gcaatgccgc gcccgccacc gacatcagga
     1681 gttcctgagc ttcctgaagc acatcgaagc caatgtcccg cgcgacctcg atctccacct
     1741 ggtg
//
LOCUS       DATAQL010000216         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1854807, whole genome shotgun sequence.
ACCESSION   DATAQL010000216 DATAQL010000000
VERSION     DATAQL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854807"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1317)
                     /locus_tag="VHS97_03700"
                     /pseudo
     CDS             complement(163..1317)
                     /locus_tag="VHS97_03700"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C1-like protein"
     gene            1550..1933
                     /locus_tag="VHS97_03705"
     CDS             1550..1933
                     /locus_tag="VHS97_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX3447327.1"
                     /translation="MRSGSRPVDDPRVYLAAERTFLAWVRTSVSLMGFGFLIARFSFW
                     IREYELAGGMATHSGQGVSATLGFGMVCVGVFVCVLAAIRHRLYVRDLERGVGNPPLH
                     VRTSLVVAGILAMVGLAIAINILML"
     gene            1947..2960
                     /gene="gnd"
                     /locus_tag="VHS97_03710"
     CDS             1947..2960
                     /gene="gnd"
                     /locus_tag="VHS97_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX3447328.1"
                     /translation="MQLGMIGLGRMGSNMVRRLLRSGQECVVYDVHPAAADPLVKEGA
                     VGGASLDEFVAKLKPPRAIWMMVPAAVVDSTVAELVSRVEPGDILIDGGNSYYHDDIR
                     RGAELKSKKIHYVDVGTSGGVWGLERGYCMMIGGEIDVVRRLDPIFAALAPGAGNSPR
                     TPGREKLPGTSEQGYLHCGPSGAGHFVKMVHNGIEYGLMAAYAEGFNILKNANIGKHK
                     ADVDAETTPLRTPELYQYDLNLADIAEVWRRGSVIASWLLDLTAQALAHSPQLAEFSG
                     RVSDSGEGRWTANAAIDEGVPAPVLSAALFARFSSRGTDEYADKMLSAMRFAFGGHLE
                     KKA"
     gene            3077..>3429
                     /locus_tag="VHS97_03715"
     CDS             3077..>3429
                     /locus_tag="VHS97_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447329.1"
                     /translation="MPMHDWTRVEAGIFHAFHHRWISAISDTLNTGLLPPDYYALPEQ
                     HAAGFGPDVLNLQGQPDQPGDESLETARYLTLLSRPRTRFMAESGAEFYRRKKSSVVV
                     RHVSGDRIVSMVEIVS"
BASE COUNT          640 a          982 c         1113 g          694 t
ORIGIN      
        1 gccggcgcgc gctttcgccg tcctcaccgc gaccaaccag gtaggctccg ccggcgacaa
       61 ggccactgac cagtcggcta gcaagctgat cgagtctgtc ggatttctga cccggcaata
      121 atctcgaaaa ccctgaggcc cggcgcctgt gtggctcgcg attcacttga atccagaccc
      181 ggacttgccg ctttcgaggt cgatccggcc ccagacgtga tcgccgctca cggggttgga
      241 tcgggcgacc tgtccgtccg agaagcgaac ggaataatcc gaaaaccgcc gatcgggccg
      301 agggtagtcg gtgctaatga actgggctcc gctcgccaga gccttgtcgc gctctttgat
      361 gtcgccggtg cgggcctggc gcgtgtctgc atcggcccgg gagcgcacca agaaaccctc
      421 gcggacgaga tgctggatac ggtcaaagtc cttgacagga tcgttgatct tgaaccaggc
      481 ggcggctgga tccgtgggct cgacgctcgc aaacatgacg cgacccttca aggcctggtg
      541 tccttcgaga tagaggtctc gcagcgagcc ttcgttgtcg agggcaaaaa ggacgagccc
      601 gcgaacctcc tcgagagccg gccatccctg ggcacggatg gcctcgggca aggtcgcgaa
      661 ccggccgcgg atccggtcgg gagtaatgat ctcgctcttg gcgaagaccg aaaggatctc
      721 ggcgtcgacg gtgtcgatct cctcgcgccc gaagcgtatc gggcgggtgg gtagagacgg
      781 aattgcagtg tctttgagct cgacctggat caagatgggg acatgacgcg ggttggcttc
      841 agaccaaccc cggacctgtt tcaaggcgtc gataaacgta gaggcggtcg agcggaaatc
      901 gacatcggga acgtgaagta cctttaatca cggcttatcg agctgtccct gggcgtttgg
      961 gtcgggaccg ggatccttct tttgaccgat caagatcttg cgcgcggacg ggctggcaaa
     1021 cagccctcct ttgggatcgg cgtagacatc gagctcgacc tggcggatgc ctagttgtga
     1081 gaattgctcg gccaaagggg ggtgggtata gtcaatcgac tcggcccggc gcgcgccacc
     1141 ggcggcgatc aggctcatca cgcctggatc gggcgcgata tgatacgagt tgtggctgcc
     1201 aatgacctgg atctggttca aacgcggctc gccggccaga gacgccgtgc tgagtagtgc
     1261 ccagcagctg acgtcaagca tgaatcctgc tgcgacgtgg attaatcgcc aaaacatgct
     1321 gttcgaccct cgtcaacgaa gagtgccaat tctcgtgcta cagtttgatc ggttttactt
     1381 caatcgtcac tgtctgaaca ggaccctgca acgccggcag atctcgcacg cttgacacac
     1441 gaaccacggg ccgtccatat tgcgcatgcg ggcgtatgtg gaggatagaa cgatcgcgga
     1501 gactgtcgca aagttagctg agactgatcg gagcgcggca acgagcgggt tgcgatcggg
     1561 gtcccgaccg gttgacgatc cgcgcgttta cctggcggcg gaacggacct ttctcgcctg
     1621 ggttcgtacg tcggtgtcgc tcatgggttt tggctttttg attgcaagat tctcgttctg
     1681 gatccgcgag tacgagcttg cgggcggcat ggcgacgcac tccggccagg gcgtttctgc
     1741 aacgctcggg ttcggcatgg tttgcgtcgg ggtattcgtt tgcgtgttgg ccgctattcg
     1801 ccatcgcctt tatgtccgcg atctggagcg gggcgtcggt aacccgcccc tgcatgtcag
     1861 gacatcgttg gttgtagccg gaatcctggc gatggttggt ctggccatcg ccattaacat
     1921 cctcatgctt taatcgcgag gcagccatgc agcttggcat gatcggcttg ggtcgaatgg
     1981 gttccaacat ggtgcggcgg ctcttgcgca gcgggcagga atgcgtggtt tacgacgttc
     2041 atccggccgc ggcggatccc ctggtcaagg aaggggccgt gggcggggca agcctcgatg
     2101 aattcgtggc caagctgaag ccgcctcggg ctatctggat gatggtcccg gcggcggttg
     2161 tcgactcgac ggtcgctgag cttgtcagcc gcgtcgagcc gggtgatatc ttgatcgacg
     2221 gcggcaactc gtactatcac gatgacatcc gccggggcgc cgagttgaag tccaaaaaga
     2281 tccattatgt cgacgtgggg accagcggcg gcgtctgggg actcgagcgc ggctactgca
     2341 tgatgatcgg cggcgagatc gatgtcgtgc ggcgactcga tccgatcttt gccgcgctgg
     2401 cccctggtgc cggaaactcg ccgcgcacgc caggtcggga aaagttgccc ggtacgtccg
     2461 agcagggcta cctccactgc ggacccagcg gcgccgggca cttcgtcaag atggtgcaca
     2521 acggcatcga atacggcctg atggccgcct atgccgaggg gtttaacatt ctcaagaatg
     2581 cgaatatcgg taagcacaaa gccgatgtcg acgccgaaac cacgcccttg cgcacaccgg
     2641 agctttacca atacgacctg aacctagccg acatcgccga agtctggcgg cgcggcagcg
     2701 tcattgcctc gtggctcttg gatttgacgg cgcaggctct cgcgcacagc ccccagcttg
     2761 ccgagttctc gggacgcgtc tctgactcgg gagaggggcg atggacggcc aatgccgcca
     2821 tcgacgaagg cgttcccgcc cccgtgttga gcgcggctct gtttgccagg ttcagctcgc
     2881 gcggaactga cgaatacgct gataaaatgc tgtcggccat gcggttcgcg ttcggcgggc
     2941 atctggaaaa gaaagcgtga gcgccggcta gagcctcggt tgacaccagt tggtttgggg
     3001 ctcatcccgt ggttgatata ctggtgacgt ggagggtgtt gcggacatac cgcaagtgtc
     3061 gaaaagagag tgtgtcatgc cgatgcatga ttggacgaga gtggaagcgg gtatcttcca
     3121 cgctttccat caccgctgga tctcggctat cagcgacacg ctcaacacgg gattgctacc
     3181 gcccgattac tacgcgctgc ccgagcagca tgccgcgggt ttcgggcccg atgttttgaa
     3241 cttgcagggt caaccggacc agccaggcga cgaatccctt gaaactgcaa ggtacctcac
     3301 gttgctctca cggccacgaa cccggttcat ggctgagtcg ggtgccgagt tctaccgccg
     3361 gaagaagagt tcggtggttg tccggcacgt cagtggagac cggatcgtat cgatggtcga
     3421 gatcgtctc
//
LOCUS       DATAQL010000217         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3229643, whole genome shotgun sequence.
ACCESSION   DATAQL010000217 DATAQL010000000
VERSION     DATAQL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229643"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(446..742)
                     /locus_tag="VHS97_03720"
     CDS             complement(446..742)
                     /locus_tag="VHS97_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447330.1"
                     /translation="MLWTIDLQADVGTASKRPHGAGLDAMSILSPAERRQLEQDGIMP
                     IEDGAFVVLRADVFERVRPLIVGEELTLDEQRRLLSELGKSVGWDDPAMDVYND"
     gene            complement(956..2431)
                     /gene="hemG"
                     /locus_tag="VHS97_03725"
     CDS             complement(956..2431)
                     /gene="hemG"
                     /locus_tag="VHS97_03725"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX3447331.1"
                     /translation="MAERMSGGAGSDRVVVIGGGLSGLATAHRIQERARTMRRPVELT
                     VLEAKDQIGGVLWTDRSDGFTLEGGPDSFITNKPWGIDLCRRLGLSDQLIATESSHQR
                     SFVVRNGRLVPVPEGFVLMAPHRLGPILTTPILSWRGKLRFLMDLVIPRRDEPSEESL
                     AAFVRRRLGREALDRLVQPLVAGIYTADPNDLSLKATLPQFLAMERDHGSLILAGWRQ
                     SGRRGARGLERQASGARYGMFVTLAEGMDTLPKALARALPEGTVRTGTAVRRISRNEP
                     VSPWLVELLNGPPIEADAVVIATEAHAAARFLDSQDPTLALQLRAIPYASSLIVNIAY
                     RRDQISHPLDGFGAVVPAIEGRPILAVSFLSVKFANRAPAGTVLLRVFIGGATQPQHL
                     ELDDQAITGLVRHQLADLIGASGEPLFARIGRHPRSMPQYILGHLDRVDTIRRHLAKH
                     SRLYLAGVAYDGVGIPDCIHAAEMTADSLLEALANPAAHAA"
     gene            complement(2607..>3341)
                     /locus_tag="VHS97_03730"
     CDS             complement(2607..>3341)
                     /locus_tag="VHS97_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX3447332.1"
                     /translation="HTTADVDRIRPLGDIAPLSYVMEAVGSIRAALPATIPLIGFAGA
                     PFTLACYVIEGAGSRHYDQAKSFMYRDPGAWHALMERLTGATALYLNGQVQAGAQALQ
                     VFDSWVGALAPHDYRKFVQPHMIKLFGLLDPSVPVIHFGTGTSTLLELQRGAGGAVIG
                     LDWRVELDQAWRQLGYDVAVQGNLDPVVLLSSPEEIETQARRILAEAGGRPGHIFNLG
                     HGILPQTPVDNVLALVDMVHAISSRS"
BASE COUNT          688 a         1054 c         1010 g          589 t
ORIGIN      
        1 acttcactcg ccccgtctga tcagatcgcc atctctgcat gggctcgcca atccgtcgtc
       61 gtcgtcgggt attgatcgat cggaggatta agatcaggaa aaagactcgc cactcaaggt
      121 ctgcccagtg cgattttcaa acaatcatcg atctgcgcca ttgtcgctcg agagataaag
      181 ccaatccgct ttacgatgag ccgctgatgg agggagaaaa ggctctcaca agccactacg
      241 gacaccgcga gcaatccgga tcccgtttcc aacgcgggat caatgacaag gcgcgttgcg
      301 ccgatcctgt tgggattgct ggtgatcagt gcgatgatcg tgttgaccga gggcaccgag
      361 tccccgagca ccactagcgc gggtctgcgt ttcgtctgcg ccgcgtcgga gaatggaaaa
      421 tcgacaatga cgatgtcacc cctcatcaat cgttatagac gtccatcgcc ggatcgtccc
      481 agccgacgct ctttccaagc tcggacagta accgccgttg ctcatcgagt gtcaattctt
      541 ccccaacgat aagtggtcgg acgcgctcga aaacgtccgc cctcaacacc acgaaggcgc
      601 cgtcctcaat cggcatgatg ccgtcttgct cgagctgccg acgttccgct gggctcagaa
      661 tactcattgc gtccaacccg gccccgtgag ggcgtttaga tgccgtgccc acgtcggcct
      721 gaagatcgat ggtccaaagc atgcgacgat ttccagcgaa cgcaggcgca acgagcttat
      781 ctcgtagcga tacattgtat ggtagagagg cttgccgtcg acatccaggc gattcgtgcg
      841 gacccgatcc ccgtcgcacg acttgagcgc aacgaccgag ggccgtcgac caattcaaac
      901 aattggccaa agccaatccg atcgatgctc aatcccgccc cgatgcgcgg tcgagtcacg
      961 cggcatgggc cgccgggttt gcgagggctt cgagcagcga atcggcggtc atctccgcgg
     1021 cgtggatgca gtcgggtatt cccacgccgt catacgcgac accggcgaga tagagccggg
     1081 agtgcttggc gagatggcgg cggatggtgt cgacgcgatc gaggtgtccc agaatatatt
     1141 gaggcatcga ccggggatgg cggccaatgc gcgcgaagag gggttcgccg ctcgccccga
     1201 tcaaatcggc cagctgatgc cggaccaagc cggtgatcgc ctgatcgtcg agttcgaggt
     1261 gctggggctg ggtcgccccg ccgataaaga cccgcaagag aaccgttccg gccggcgccc
     1321 gattggcgaa cttgacactt aaaaacgaca ccgccaagat cggccggccc tcgatggccg
     1381 gcactaccgc cccgaagccg tccagaggat gactgatctg gtcgcgccgg taggcaatat
     1441 tgacgatcag tgacgatgcg tatgggatcg cgcgcagctg gagcgccagg gtcggatcct
     1501 gcgagtcgag aaatcgcgcc gccgcgtggg cttcggtggc gataacaacc gcgtcggcct
     1561 cgataggcgg gccgttcaga agctcgacca gccagggtga caccggctcg ttgcgactga
     1621 tccggcggac cgccgtaccg gtacgaaccg tgccctcggg aagcgcccgg gccagggcct
     1681 tgggaagcgt atccatgccc tcggcgagcg tcacaaacat cccgtaccgc gcgcccgacg
     1741 cctgccgttc gaggccacgg gccccgcggc ggcccgactg gcgccagcca gccaagatca
     1801 ggctgccatg gtcgcgctcc atcgccagaa actggggtaa ggtcgccttc aggctcaggt
     1861 cgttggggtc ggccgtgtag atccccgcga ccaagggctg aaccaggcgg tccagagcct
     1921 cgcggcccag ccggcggcgc acgaaggcgg ccagactttc ctcgcttggt tcgtcgcggc
     1981 gcgggatgac taggtccatc aagaaacgga gcttaccgcg ccaggaaagg atcggcgtgg
     2041 tcaaaatcgg accaagacgg tgcggcgcca tcaagacaaa gccttccggc accggcacta
     2101 accggccatt gcggacaacg aacgaacgct gatggctcga ctcggttgcg atcaactgat
     2161 ccgaaagccc gagacgacgg cagaggtcga tgccccaggg cttgttggta atgaacgagt
     2221 ccggcccccc ctcaagcgta aaaccgtccg agcgatcagt ccaaagcacg ccgccaattt
     2281 gatccttcgc ttccagaacg gtcagctcga caggcctgcg cattgtccgc gcgcgttcct
     2341 ggatccgatg cgcggtcgcc agaccggaga gcccgcctcc gatcacaacc acacgatcag
     2401 accccgcacc gccagacata cgctctgcca aattggttca ctcccacgcg attgcgcgat
     2461 tctgtatctt gtttgcctat cttatttacg gggtagttgt caaggggttg gggcgcaaaa
     2521 acttacgccg tctcgactgt tcgtaacccc tatataccag gcgctcgatc gcggcttttg
     2581 atcaatggcg gccggcgaag cgggcgttac gatcgactgg aaatggcgtg aaccatgtcg
     2641 accaatgcca agacgttgtc gacgggagtt tgcggaagta taccatgccc gagattgaag
     2701 atatggcccg gccggccgcc ggcttccgcc aggatgcgcc gggcctgcgt ctcgatctcc
     2761 tcaggcgacg atagcaagac gaccggatcg aggtttccct ggacggcgac gtcataaccc
     2821 agctgccgcc acgcttgatc gagctcaacc cgccaatcga ggccaatgac ggcgccgccg
     2881 gcaccgcgct gtaattcaag cagtgtcgac gtaccagtcc cgaaatgaat gaccgggacc
     2941 gacggatcca gcaggccgaa gagctttatc atgtgtggct gtacaaactt gcggtagtcg
     3001 tggggagcca acgcgcctac ccagctatcg aacacctgga gtgcctgggc tcccgcctgg
     3061 acctggccat tcaagtacag cgcggtcgcc ccggtcaatc gctccatgag tgcatgccag
     3121 gctcctgggt ctctatacat gaacgacttg gcctggtcgt aatggcgcga tcccgctccc
     3181 tcaatcacgt agcacgcaag ggtaaacggt gcaccggcaa agccaatcag cggaattgtc
     3241 gccggcagcg ccgcccgaat ggaccctacc gcctccatca cgtacgagag cggcgcgatg
     3301 tcgccgagcg gccgaatgcg gtcaacatcg gcggtggtgt g
//
LOCUS       DATAQL010000218         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1549280, whole genome shotgun sequence.
ACCESSION   DATAQL010000218 DATAQL010000000
VERSION     DATAQL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549280"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..835)
                     /locus_tag="VHS97_03735"
     CDS             complement(110..835)
                     /locus_tag="VHS97_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX3447333.1"
                     /translation="MISTIDTVITAETPEGVAISMRAAGFPVRCMAFLIDAAIRYTIV
                     STVATGLNFGGRVGQGVLLIIVFLILWLYPVIFELTPAAATPGKRAMGIQVIMANGLP
                     LTPAGCLIRNLMRAVDMLPLLYGFAIVSMLLRRDARRLGDLAAGTLVVYRDRASPTSD
                     FGPGEPLRPPLPLTLRQQTAIAAFGWRVTRLTSERAEEIAVLAADAVPGAKDTAVATR
                     LVGIARWQHGQRGVIPRDAAKQT"
     gene            complement(832..1365)
                     /locus_tag="VHS97_03740"
     CDS             complement(832..1365)
                     /locus_tag="VHS97_03740"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX3447334.1"
                     /translation="MATDSSNLYAPPRSAVKDVDGDDALELASRSARLGAVILDGLIG
                     FIWFVPAYITNFAMIAQQSRGNPLAVWINLARTGGWFYLGLIGALVVLAIDLTLLARN
                     GQTIGKKLLGIKVVRVDRSPVSLFRVFFLRYVCNTFLTLIPLVGSLYSLVDSLMIFSE
                     SRRTVHDRIADTIVIKA"
     gene            1599..>2081
                     /locus_tag="VHS97_03745"
     CDS             1599..>2081
                     /locus_tag="VHS97_03745"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HEX3447335.1"
                     /translation="MYRYPTDIYTEPEPDTDTLANLGPLTGMAGIWTSAAGQDVNPKR
                     DGPEHQRFIERYELQPIDAQTNGPQLFYGLRYHTRIVKPDDPETFHDQVGHWLWEPAT
                     HAILLTLSIPRGQTAMAVGQATPDAKSFTLEAVRGSLTNGIISNPFLEQAFRTDRYTI
                     K"
BASE COUNT          450 a          651 c          627 g          353 t
ORIGIN      
        1 cgatagaggg caaggaaccg gtcgggatcc agttggcgtt ggtggcgtcc gagttcgctt
       61 tcgagttcgt tccagctggc ctggttcgcg gcttgaaagt cgaacggcgt cacgtctgct
      121 tcgccgcgtc tcgcgggatc acgccccgct gaccgtgttg ccaacgcgca atcccgacca
      181 ggcgggtcgc caccgcggtg tctttggcgc ccggcacggc gtcggcggcc aaaaccgcga
      241 tttcctcggc gcgctccgat gtcagtcgag tgactcgcca gccgaaggcg gcgatggccg
      301 tctgctgacg gagggtcaac gggaggggtg gccgcagggg ctcgccggga ccgaaatcgc
      361 tcgtcgggga ggcccgatcg cgatacacga cgagcgtgcc ggccgctagg tcgccgagcc
      421 ggcgggcgtc ccggcgcaac agcatggaca cgatcgcaaa gccatacaat agcggcagca
      481 tgtccaccgc gcgcatcaag ttgcgaatca agcagcccgc gggggtgagg ggcaggccgt
      541 tggccatgat cacttggatg cccatcgcgc gctttcccgg cgtggccgcc gccggcgtca
      601 attcaaagat gacgggataa agccacaaaa tcaggaaaac gataatcagc agtacacctt
      661 ggcccacacg gcccccgaaa ttgaggcctg tggcgacggt ggaaacgatc gtgtagcgta
      721 tcgctgcgtc gatcagaaaa gccatgcacc gcacgggaaa gccggcggca cgcatcgaaa
      781 tcgcgacgcc ctcgggcgtt tccgccgtaa tgaccgtgtc tatggtcgag atcaagcttt
      841 gatgacgatg gtatcggcga tgcggtcgtg caccgttcgt cgcgactcgc tgaatatcat
      901 cagagagtcg accaaggaat acagggaacc gacgagcggt atgagcgtca gaaaagtatt
      961 gcagacatag cgcagaaaaa agactcgaaa caacgacacc ggcgatctgt cgacgcgcac
     1021 gactttgatg ccgagcagct tcttgccgat ggtctggcca ttgcgcgcca acaaggtgag
     1081 atcgatcgcg agtacgacca gcgcgccgat gaggccgaga taaaaccaac cgccggttct
     1141 tgcgagattg atccagaccg ccagtggatt gccgcgcgac tgttgtgcaa tcatcgcaaa
     1201 attcgtgatg taggccggca cgaaccagat gaagccgatg agtccatcga gaatgacggc
     1261 ccccaagcgc gccgacctcg acgctagttc gagcgcatcg tcaccatcaa cgtctttgac
     1321 cgcacttctg gggggagcat ataagttgct cgaatccgtg gccatattcg ctccttgcgc
     1381 cgatcgagtg cctatgatac cccggtgtcg cgagccggtg gcaatcggtt tgaaatcctg
     1441 tgcgcgacct cacagcctca gggtgcgaga cgccggagtg atcaacgaga accagcgccc
     1501 cgaacaagct agtacgataa gccgctttgg gcgggcgccc cgatcaggcg ggcgctgcga
     1561 tcccagaact gcaaaccgat tcctcgcgcg gagttctcat gtatcgctac cccaccgaca
     1621 tctacaccga acccgaaccg gatactgaca cgctggcaaa cctcgggcca ctgaccggca
     1681 tggccggcat ctggaccagc gccgccggcc aagacgtcaa tccgaagcgt gacggtcccg
     1741 agcaccagcg gttcatcgag cgctacgaac tccagccgat cgacgcccag accaacgggc
     1801 cgcaactttt ctacgggctg cgctatcaca cacgaattgt gaagccggac gatccggaga
     1861 cctttcacga ccaggtcggc cactggttat gggaaccagc cactcacgca atactgctga
     1921 cgctgtcgat tccccgcggc caaaccgcga tggccgtcgg ccaggcaacc cccgatgcga
     1981 agtcattcac gctcgaagcc gtacgtggtt cgctcactaa tggcatcatc tccaatcctt
     2041 ttctcgagca ggcgtttcgc accgatcgct acacgatcaa g
//
LOCUS       DATAQL010000219         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_633516, whole genome shotgun sequence.
ACCESSION   DATAQL010000219 DATAQL010000000
VERSION     DATAQL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633516"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1019)
                     /locus_tag="VHS97_03750"
     CDS             complement(42..1019)
                     /locus_tag="VHS97_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3447336.1"
                     /translation="MNSTQITEPAINELDELLSLSNRLLDNVALVVLGKPDVIRLAVV
                     ALLAEGHVLIEDVPGVGKTLLARAIAASIDCSFRRIQFTPDLLPSDVLGSSIYHAPSG
                     EFVFKPGPLFANVILADEINRTTPRTQSALLEAMSDRQVSIEGQTYSLTPPFIVLATQ
                     NPYEFEGTYVLPESQLDRFMLRLRMGYPIRAEERKVFASHRNGEPVEMLSPVLTAQEV
                     LQLQHGVRGVQVDDAITEYLLDIVHATRRSEDLHVGVSTRGALTLYRAAQSLALVSGR
                     NYVVPDDIKSLVVPVLAHRVVGKSFLQAGEFGAAEAIIHDLVDRIRVPA"
     gene            1214..2287
                     /locus_tag="VHS97_03755"
     CDS             1214..2287
                     /locus_tag="VHS97_03755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3447337.1"
                     /translation="MTGESIKVLILAGRLNRHDGGWPIGPLLDRLERRGCDVQIVCLS
                     DGGGLADLPRVIELRVLGNRWLRSFALRSFWSNSPLERPDLVHAVDDEMGDVALALAE
                     TAGLPYYQTVSRFGTIARGLRLSRRWCRGIVATSLDLGQALIDELGVPADRIAVIPPG
                     LAPPAHHFGGDGTGKVPVIGTGGPLEEASGTIVFLEAARLVLDAGHDVEFVIASQGSQ
                     QVILRHRAKRLQIAERVTVADYPSFSAEFWSVLDVYCQPAVVPSTGRTLIQALGHAIP
                     CIATDVKGLSGLIDSGENGLLVPRSDPNTLAKAITSLLDDPVKAQRLGSAALDRVRAC
                     FDPEREADRLADLYHQAALARCV"
     gene            complement(3311..3436)
                     /locus_tag="VHS97_03760"
     CDS             complement(3311..3436)
                     /locus_tag="VHS97_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447338.1"
                     /translation="MPVWALELPAPAWVPVEVVVPAWALELPVAAWLPVDVVVLV"
     gene            complement(3455..3829)
                     /locus_tag="VHS97_03765"
     CDS             complement(3455..3829)
                     /locus_tag="VHS97_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447339.1"
                     /translation="MPDPAPALPEFADDEVVVDVCALGAGEPALLEFAEDEVDVCADE
                     LPEPESPAAVLAFVDVEVDACAPGDAGVGVVAVDGFVAVLPAVVAVVVPVVVCALELP
                     DPAWVPVEVVVPVWALELPAPA"
BASE COUNT          800 a         1286 c         1140 g          705 t
ORIGIN      
        1 gccccgcgga gccaaaaagc gattgcggtg agcctgattt ctcacgccgg cactcgaatg
       61 cggtcgacca gatcgtggat gatcgcctcg gccgctccga actctcccgc ttgaaggaac
      121 gacttgccca cgacgcggtg agccagtacc gggacgacca gtgacttgat gtcatccgga
      181 accacgtaat tgcgacccga aaccagtgcc aggctctgag cggcccgata gagcgtcagt
      241 gcgccgcgcg tgctcacccc cacgtgcagg tcttcactgc ggcgcgtcgc atgcacaatg
      301 tcgagcaggt actccgtaat cgcatcatcc acctgcaccc cccgcacgcc atgttggagc
      361 tggagaactt cctgcgcagt caagaccggg ctcagcatct cgactggctc gccattgcga
      421 tgactcgcaa agaccttgcg ttcctcggcc cggatcgggt atcccatccg cagccggagc
      481 atgaagcgat cgagctggct ctccggaaga acgtaggttc cctcgaattc ataggggttt
      541 tgagtcgcca acacaataaa ggggggcgtc aacgaatacg tctgtccctc gatcgaaacc
      601 tgcctgtcgc tcattgcctc caggagcgcg ctctgggtac gaggggtggt gcgattgatc
      661 tcgtcggcca gaatcacgtt ggcaaaaagc ggtccgggct tgaacacaaa ctcgccggac
      721 ggagcgtgat agatgctcga tcccagcaca tccgacggca gcaagtcggg agtaaactgg
      781 attcgccgga acgaacagtc gatactggcg gcgatggctc gggccaagag cgtttttccc
      841 acaccgggaa catcttcgat cagcacgtgt ccttcggcca gaagcgcgac gacggcaagc
      901 cgaatgacgt ccggcttgcc caggacgacg agtgcaacgt tgtcgagcaa gcgattcgag
      961 agagatagaa gttcatcgag ttcattgatc gcaggttcag tgatttgggt agaattcatg
     1021 cggtacgctc aacgaacggt gcggtgcgac tggtccaacc ttggtccttc gcgatgaata
     1081 tactataaac catcgacgca ccgattccaa cggctggatg gatgcggggc cgcctgaaca
     1141 gtcagtgcac ggcggttcaa ggacgactga cactcgcggg gggccgcgct cgagcccggc
     1201 ctagcttcaa ttcatgacag gcgagtcaat taaggtcttg attctggccg gtcgtctcaa
     1261 ccggcacgac ggagggtggc cgatcggccc tctgctcgat cggctagaac gtcgcggctg
     1321 cgacgtccag atcgtttgcc tgtccgatgg ggggggcctg gccgaccttc ctcgggtgat
     1381 cgagcttcgc gtgctcggaa accgctggct acgcagcttc gccctccgga gcttttggtc
     1441 caacagtccg ctggaacgcc ccgacttagt gcatgccgta gacgacgaga tgggcgatgt
     1501 tgccttggca ttagctgaga ccgccggtct tccctactat caaacggtct cacgttttgg
     1561 gaccatcgcg cgcggcctga ggctcagccg ccgctggtgc cgcggaatcg tagcgacgag
     1621 cctcgatctc ggccaggcct tgatcgacga actcggcgta ccggccgacc ggatcgcagt
     1681 cattccgcca ggattggcac cacccgcgca ccatttcggc ggggacggga ccgggaaggt
     1741 gccggttatc ggcaccggcg gacctctcga ggaagcttcg ggaacgattg tcttcctgga
     1801 agccgctcgt ctcgtgcttg acgccggtca cgacgtcgag tttgtaatcg ccagtcaggg
     1861 gagccagcag gtcattctcc gccaccgcgc taagcgcctc cagattgccg agcgggtgac
     1921 ggtggccgat tatcccagct tcagtgccga gttttggtcg gtgctggacg tgtactgtca
     1981 accagccgtt gtgccgagca ccggccggac cttgatccag gcgctcggtc atgccattcc
     2041 ctgcattgcc accgatgtca aggggctctc cggtttgatc gactccggcg aaaatggcct
     2101 gctcgtcccc cgatccgatc ccaacaccct ggcgaaagcg atcacttccc ttctcgatga
     2161 cccggtcaag gctcaacgac tgggctcagc cgcgctcgac cgtgttcgcg cctgcttcga
     2221 tcccgaacgt gaggcagata ggctcgctga cctctatcat caagcggcct tggcgcgctg
     2281 cgtctgacca atatgccgtg atgccatcgt gcagtaactt gttttattct aacaatttat
     2341 tgcgaaccag ccgccatagg cttccagacg taatcccggc gcattcatga tcagctcgcc
     2401 ccatgccaca aaaaaaattg gtacatgtgg cacacgtcgg gccccgttgt tcatcatagg
     2461 accgcacgag ccgcgttcaa tgtcggcgca tcgtggcgaa aggtcggaca gccggccagg
     2521 tcgacttccc tcgtccgtgt cgctgtgtgc ggatttgcgg aacgagcatg ggagttcatt
     2581 atttgattag cccaggaggg gccatgttga ccaaggatgg catcaatcaa gctggtcgtg
     2641 gagttgtccg ccgccgcgtc aaattcgcgg ccggcgtcga aaacctggaa gggcgtatgc
     2701 tcttgtcgat ctttcgccat caggcttccc acgccgctac tcatatgacc gcgcagacca
     2761 ctgccgttgc tcagcaggcc acccaggccc acagtgtttc tcgtccccac gctcagcacg
     2821 ccactcaaca tcgccagagt gctatcgggc gcatgaaaat tgtggccggc ggcgcgacag
     2881 caagtgcggc acccacgatc accatccagc cgacgttcaa tattcagccc gcgatcaacg
     2941 tccagcccac gctcagcggc ggaacggcca gtacccagag ctcgtcaaat acgtccgcca
     3001 gcacggcccc gacgccgacc acgacttctg tctcggcgtc gggctctcag acgaatggcg
     3061 acacaaccaa ctcatattcg accaaatggt cgtcgacatc cgacaagccg acttcgcctg
     3121 acacgaaatc caccggcggc tcctcgacct tgcagaccaa caactcatcg acgaccacct
     3181 caccttcgtc aacgccatcg acgccctcga caacgccgtc ctcaccgcca acgattaccg
     3241 gcgggtcaaa cgcacaggcg ggcccaacaa cgtcgacggg aagccaggcc gattccggcg
     3301 gctcgggcgc tcaaaccagc acgacgacgt caaccggcag ccaagcggcc actggcagct
     3361 caagcgccca ggccggcacg acaacctcga cggggaccca ggcgggtgcg ggcaattcaa
     3421 gcgcccagac cggcacgacg acttcgacgg gatctcaagc gggtgcgggc aattcaagcg
     3481 cccagaccgg cacaacaacc tcgacgggaa cccaggcggg atcgggcagt tcaagtgcgc
     3541 agacgacaac gggaacgacc accgcgacaa cggcgggtaa tacggcgacg aacccgtcca
     3601 ccgccaccac gccgacaccc gcgtcaccgg gcgcgcaagc gtcgacttca acgtctacaa
     3661 acgccaacac cgccgccggc gattccggct caggcaattc gtccgcgcag acatcgacct
     3721 catcttcggc gaattccagt aacgcgggct ctccggcgcc tagcgcgcag acgtcgacca
     3781 cgacctcatc gtcggcgaat tcaggcagcg ccggggcggg atccggcact gcgggcgcgc
     3841 aaacttcgac ttcttcgtcg acctcggcct aaaggtgatc cgtcagtacc actcgccgcc
     3901 tttcaggcgg ggatcgcgac tgtgcaccac g
//
LOCUS       DATAQL010000220         7033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5213642, whole genome shotgun sequence.
ACCESSION   DATAQL010000220 DATAQL010000000
VERSION     DATAQL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5213642"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1235
                     /locus_tag="VHS97_03770"
     CDS             441..1235
                     /locus_tag="VHS97_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3447340.1"
                     /translation="MSVFRDNLFVGSVALVTGGGTGIGRGIAEALARNGANVAIMSRK
                     QDKLTAAAEQIAAATGRRCVPLVADVRQPEAVEPAVNRVVEELGGLDILVNNAAGNFL
                     CPSADLSPNGFGTVIDIDAKGTWNVSRAAYHARLKHHGGQILNISATLHYGGTPGQAH
                     VAAAKAAVDALTRTLAVEWGTQNIRVNAIAPGPIGDTEGARRLFAGEAADMLRGAIPT
                     RRLGTIDDIVNLALFVLSEAGTNINGAILVSDGGLHLVGRFGAGSL"
     gene            1284..3212
                     /locus_tag="VHS97_03775"
     CDS             1284..3212
                     /locus_tag="VHS97_03775"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HEX3447341.1"
                     /translation="MAREQDQFLDVVDRDTAERRWWSTLCPEVLASEEIALAEALTRV
                     LAIDIAAPVDVPPFDRSNVDGFAVQAQDTFGAAEERPRALLINDEEVTTGRVPTTTLL
                     PGYATSIATGAVVPRGADAVVMIEHAYVDGTRLRVVRPVAPGAGITFAGTDIARGERI
                     LRRGSVLTSRETGILAAIGMGHVTVVRQPRVGIVSTGDEIIAPGEPLRPATIHDCNST
                     LIADAVRELGAEPVVLGIVADDDHALDAVLEGGLARCDVVLLSGGTSKGAGDLCYRVL
                     ARRDPGIIVHGVALKPGKPICLGAVGKKPVVILPGFPTSAIFTFHEFVAPLLRFLSGR
                     RAFEPATTTARMPFRYNSETGRTEYLLVNLVEGPAGLAAYPLGKGSGSVTTFSLADGF
                     LTIPRTQEFIDAGEAVNVFAIGRGTAPCDLVVIGSHCTGIDLILGILNERGFTSKTMW
                     VGSHGGLTAVARGECDVAGIHLLDPDSNQYNRSFVPPRATLLAGYGRMQGIGHRPGDP
                     RFEGRVAAAIVATTARDPDCFMVNRNRGSGTRILIDRLLGGLRPCGHAVEARSHNAVA
                     AALRQGRADWGLLIEPVARAYDLAFIPLGAEQFDFLIPEDRWDRLAVAAFRECLASPS
                     VRLRLVQAGFQLDHEELS"
     gene            3209..3826
                     /locus_tag="VHS97_03780"
     CDS             3209..3826
                     /locus_tag="VHS97_03780"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX3447342.1"
                     /translation="MSFGAVVLCGGESRRMGRPKAWLPFGEELMLERVVRLAGTVAQP
                     MIVVAAPGQELPALAADVAIVRDSVSGRGPLQGLAAGLAAFDNSVDLIYATATDVPFL
                     EPRWMTRLVELAAGYDLVIPHVAGYYHPLAALYRPAAVLPAIESLLLADRLRPVFLVE
                     AVKTRVVDEGEMRAVDPALDTLRNLNYPEDYERALADAGLGRTRS"
     gene            complement(3857..5449)
                     /locus_tag="VHS97_03785"
     CDS             complement(3857..5449)
                     /locus_tag="VHS97_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX3447343.1"
                     /translation="MPIKVANIRLELDESEDGLPAKIAARLGVPRDAVVQWRILRKSL
                     DARGHDDIHFNLAAAVDVPEDELKRIGASPGLEPYVPERFQWPEPGSMPLSHRPVIVG
                     AGPAGLFAGYLLAKDGYRPLILERGRAVKDRVADVRRFDENGPLDPESNYLFGEGGAG
                     TFSDGKLTSRGSGPDVTRVLEILADCHGKPSIVYEHRPHLGSNRLPLVVRTLRRKVEE
                     LGGEIRFSCRVEDLDIADGRLRGLSTSSGYIAAELAVLAIGHSARDTYGVLLRRGVPI
                     EAKPFQLGVRIEQPQAAIDGVRYGPNAGHPALGAADYSASVRAGRRDLFTFCMCAGGY
                     VMPSVSDPGYFCTNGMSESRHDSPFANSGLVVTIEPAETGSAHPLAGVHYQQRVERAA
                     YVAGGRSYKAPIQWARDFLAGRQSRGKIPSSYRRGTLPVDLALLLPEAVMESLMIGLP
                     AMDRRFGMRFLRDATLTGPEARGSSPVRIIRDPQTRQSPTVAGLYPCGEGAGYAGGIV
                     SAAVDGVRTARAIVATYANALA"
     gene            complement(5536..>7033)
                     /locus_tag="VHS97_03790"
     CDS             complement(5536..>7033)
                     /locus_tag="VHS97_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX3447344.1"
                     /translation="KVIPGIDHRLAPGDRINICDVEFLFYPQLPADSPSKEPGDIMIV
                     TEGENLEAPHLHTLDASRSSAMASMIKPEVKLKAILEIARNLSTELKIDTVAPKILDS
                     LMELFPQAERLFLVLVDPETKRLIRKAFKYKPAKKSIFSTIIPADEIPMSISRSIVNH
                     VLGQKKAVLSQDAGADKNLPTSASIADLKIRSVMCVPLLTPDREALGIIQLDTSDRKQ
                     FHQEDLDVLAAVAGQAAIAIQNAQMHESLLERERVNRDLKLAEQVQKRFLPQSVPAIP
                     GYEFFAHYDPAYEVGGDYYDFVPLPGSRVAVAVGDVSGKGVAAALMMAKFSGDTRYCI
                     LTENSPAAASNELNSLLFSAAIEEKFITLSLSVLDTEKRTLSITSAGHLPVMVRRVNG
                     TVDEVGEEIAGFPLGIVPESDYKQIDVKLYPGDVVAVFSDGVTDARNLREELYDSRDK
                     RRLIKKLAETAGGPETVGRALLQDIREFSAGHNQVDDITLICFGPLAR"
BASE COUNT         1216 a         2125 c         2343 g         1349 t
ORIGIN      
        1 cagcttggga ggaccacggc accgagaagg ttggcgaagc gctgggagtt ttcgagctcg
       61 gccgcgaggt gctcgacagt cgccgccacc agatccagcc gggccgtttg catgcacagt
      121 gtctcgatac ccacgatctc aaccttcccg accggggtcg tcgcggaggc gtcccgccga
      181 gtgtcggcgc gtgcacttgc cgcttgttca cgttgaatat aacgaaaacc cgtccggaaa
      241 aagagcgcgc aatccagacg cgcgagacgt gacgaattgt tcgcagtgcg gtctctgata
      301 ggataggttc ggcccgagcg gccaaggtgt caccgtccgc agacctcggt cttcggtcgt
      361 cgtgcgtgat cgtcgtgcgt atctgcagca agggcggctt cggccccgcg ttttgcctct
      421 atttgttgaa gaggtttggt atgagcgtct ttcgggacaa cctatttgtg ggatcggtgg
      481 cgttagtcac ggggggcgga accggcattg gccgcggtat tgccgaggcc ttggccagga
      541 acggtgccaa cgtggcgatc atgagccgga aacaagacaa gttgacggcg gcggcggaac
      601 agatcgccgc ggccaccggg cgccgctgtg taccgctggt cgccgacgtc cggcagcccg
      661 aggcggtcga gccggctgtc aatcgcgtcg tcgaagagct cggtggattg gatatcctgg
      721 tcaacaacgc cgccggcaat ttcctctgcc cgtcggccga tctgagcccg aacggctttg
      781 gcacggtgat cgacattgac gccaagggga cctggaacgt gtcgcgggcg gcctatcacg
      841 cgcggttgaa gcatcatggg ggccagatcc tcaacatcag cgcaaccttg cactatggcg
      901 gcacgccggg tcaagctcac gttgccgccg ccaaggcggc cgtcgacgcg ctcacccgca
      961 cactggccgt cgagtgggga actcagaaca ttcgggtcaa cgccatcgcc cccggcccca
     1021 tcggtgatac cgaaggggca cggcggctct ttgccggcga ggcggccgat atgctccgcg
     1081 gggctatccc tacccgccgt ctgggcacga tcgatgacat cgtcaacctc gccctgtttg
     1141 tgctgagcga ggccggaacc aatatcaacg gtgcgattct ggtttccgat ggcggcctcc
     1201 acctggtagg ccgcttcggt gctgggagtc tctaggaact cagtctggcc actcggcaca
     1261 cgtaccgaca actcggggcg ctcatggctc gagagcaaga ccagtttctg gatgtcgtgg
     1321 accgcgatac cgccgagcgg cggtggtggt caacgctttg ccccgaggtg ctcgcatccg
     1381 aagagatcgc cctggccgag gcgctcacgc gggtcctggc gatcgatatt gccgccccgg
     1441 tcgacgtccc tccgtttgat cgttccaacg tcgacgggtt cgccgtacag gcacaagaca
     1501 ccttcggcgc ggccgaggaa aggccgcgcg ctctcttgat caacgatgag gaggtgacga
     1561 cgggacgggt gccgacgacg actctcctgc ctggatatgc cacctcgatc gcgacaggcg
     1621 ccgtcgttcc ccgcggtgcc gatgcggtgg tcatgatcga acacgcgtat gttgacggca
     1681 ccagactgcg ggtcgttcgt ccggtcgcgc cgggggccgg aatcacgttc gcggggacgg
     1741 acatcgcgcg gggtgaacgg atcctccggc gcgggtcggt gctcacgtca cgcgagaccg
     1801 gtattctggc cgccatcggc atggggcatg tcacagtggt ccgccagccg cgcgtgggga
     1861 tcgtctccac cggtgacgag atcatcgcgc ccggagagcc acttcgaccc gccacgattc
     1921 acgattgcaa ttcaaccttg attgccgacg ccgtccgcga gctcggtgcc gagcccgtgg
     1981 ttctcggcat cgtcgcggac gacgaccacg ccctggacgc agtcttggaa ggcggtctcg
     2041 cgcggtgtga tgtggtgctc ttgagcggcg ggacgagcaa aggggcgggg gacctttgct
     2101 atcgcgtcct ggctcgacgc gatcccggga tcatcgtcca cggagttgcg ctcaagccgg
     2161 gcaaaccaat ctgcctggga gcggttggca agaagccagt ggtcatactg cccggatttc
     2221 caacctctgc gattttcaca ttccatgaat tcgtagcgcc cctgcttcgt ttcttgagtg
     2281 ggcgccgcgc gtttgagccg gcgacgacga ccgcacgcat gccttttcgc tacaacagcg
     2341 agactggtcg aaccgaatac ctgctggtga atctggtgga aggcccggcg ggattggctg
     2401 cctatccgct cggcaagggg tctggatcgg tgacgacctt cagcctggcc gatggttttc
     2461 tcaccattcc ccgcacccag gaatttatcg atgcgggcga agccgtcaac gtcttcgcga
     2521 ttggccgcgg gacggcgccc tgcgacctgg tggtgatcgg gtcgcattgt acgggcatcg
     2581 atctgatcct gggcatcctc aacgaacggg gcttcacgtc caagacgatg tgggttggtt
     2641 cgcacggcgg attgactgca gtggcgcggg gcgaatgcga cgttgcggga attcatttgc
     2701 tcgacccgga tagcaatcag tacaaccgct cgttcgtgcc accgcgcgcg acacttttag
     2761 ccggctatgg aaggatgcag ggaatcggtc atcgcccggg cgacccgcgt ttcgagggtc
     2821 gggttgccgc cgcgatcgtc gcgaccactg cacgtgatcc cgattgtttc atggtcaacc
     2881 gcaatcgcgg cagcgggacg cggatcttga ttgatcgcct gctgggcggc ctccggccgt
     2941 gcggtcatgc ggtcgaggcg cggtcgcaca acgcggttgc cgctgccctg cggcagggac
     3001 gagcggactg ggggctcttg atcgagccgg tcgcccgggc ttacgacctg gcattcatcc
     3061 ccttgggcgc ggaacaattc gatttcttga tccccgaaga tcggtgggac agactagcgg
     3121 tcgcggcgtt ccgcgagtgc ctggccagtc cgtctgtccg cctccgtctc gtccaggccg
     3181 gatttcagtt ggatcatgag gaactctcat gagcttcggc gcggtggtct tatgcggggg
     3241 tgaaagccgg cggatggggc ggcccaaggc ctggctgcca ttcggtgaag agctcatgct
     3301 cgagcgcgtc gtgcggctgg ccgggactgt cgcgcagccg atgattgtgg tggccgcccc
     3361 aggtcaggag ctgcctgcac tagctgccga tgttgccatc gtgcgcgact cggtctccgg
     3421 gcgtgggccg ctgcaaggat tggccgcggg attggcggca ttcgacaact cggtcgactt
     3481 gatttatgcg acggctaccg acgttccgtt cctcgagcca cgctggatga cacggcttgt
     3541 cgagctggcc gccggctacg acctggtcat tccccacgtg gctggctatt accatccgtt
     3601 ggcggcgctc tatcgaccgg ccgcagtctt gccggcgatc gagagcttgc tccttgcaga
     3661 ccgcttgcga ccggtcttct tggttgaggc cgtcaagact cgagtcgtcg acgagggcga
     3721 aatgagagct gtcgatcctg cgctggacac gctcaggaac ttgaactatc ccgaggacta
     3781 tgaacgggca ctggctgacg cgggattggg gcgcacacgt tcgtagcggc cggttcaatt
     3841 ctcaggccgc gagagtttat gcgagcgcgt ttgcgtatgt tgccacgatc gcccgcgcgg
     3901 tgcgcacacc gtcgacggcg gcgctcacga ttcccccggc gtagccggct ccctcgccgc
     3961 aggggtaaag ccccgccacc gtcgggcttt gccgggtttg cgggtcgcgt atgatgcgga
     4021 caggcgagct gccgcgcgct tcgggcccgg tgagtgtggc gtcgcgcagg aaccgcatgc
     4081 caaagcgtcg atccatcgcg ggaaggccga tcatcagcga ctccatgacc gcctcgggga
     4141 gcaagagcgc cagatcgacc ggcagcgtgc cgcggcgata gctgctgggt atcttacctc
     4201 ggctctgccg gccggccagg aaatcgcgag cccattggat cggtgctttg taggaacgtc
     4261 cacctgctac gtacgccgca cgctcgacgc gctgctggta gtggacgccg gcaagcgggt
     4321 gcgcgctgcc tgtttccgcc ggctcgatgg tcacgaccag gccgctattg gcgaacggtg
     4381 aatcgtgccg gctctcgctc atgccgttgg tgcagaagta tcccgggtcg ctgacactgg
     4441 gcatgacgta gccgccggcg cacatgcaaa aggtaaacaa gtcgcggcgg ccggcacgga
     4501 ccgacgcact ataatcggcc gcgcctaagg ccgggtggcc ggcgttggga ccgtatcgga
     4561 ccccgtcgat cgcggcctgg ggctgttcga tccgcacgcc gagttgaaac ggtttcgctt
     4621 cgatcggcac gccgcggcgc aaaagcactc cgtacgtatc gcgagcgctg tggccgatcg
     4681 ctaaaacggc taattcggcg gcgatgtagc ccgaggaggt tgagagaccc cgaagccgac
     4741 cgtcggcgat gtcgagatcc tcgacgcggc acgaaaaccg gatctcgccc cccagctctt
     4801 caaccttgcg tcgcagcgtc ctgacgacca gtggcaagcg gttagaccca aggtgcggcc
     4861 ggtgttcata gacgatcgat ggcttaccgt ggcagtcagc gaggatttcc agtacacggg
     4921 taacgtccgg gccgctgccg cgcgaggtga gcttaccgtc gctaaatgta ccggcccccc
     4981 cttcaccgaa gaggtagttg ctctcagggt ccagcggccc gttttcatcg aaccggcgaa
     5041 catcggcgac gcggtccttg acggcccgcc cgcgctcgag gatcaagggc cgatagccgt
     5101 ctttcgcgag caaatagccg gcaaagagcc ccgcgggacc ggcccctacg atgacgggcc
     5161 ggtgcgagag gggcatcgag ccgggctccg gccactggaa tcgttcggga acatagggct
     5221 ccaggcccgg cgaggcaccg atgcgcttga gctcgtcttc ggggacatcg acggcggcgg
     5281 ccaggttaaa gtggatgtcg tcgtgcccgc gggcgtcaag gctcttgcgg agaatacgcc
     5341 attggaccac cgcgtcgcgc ggaaccccca gccgggctgc gatctttgcg ggcaatccgt
     5401 cttcggattc atcgagctcc aggcgaatgt tggcgacttt gatcggcatg gctggctagt
     5461 tagaccggtc tgcgaccgcg ggcatcttgc ccatcaaatg gatgctggcg ctgggctcgt
     5521 gccttccagc ggacatcagc gagccagcgg tccaaaacag atcagggtaa tgtcatcgac
     5581 ctggttatgg ccggcggaaa actcgcgaat atcctggagc aaggcgcgtc cgaccgtttc
     5641 cgggccgccg gccgtttccg cgagcttttt gatcagccgg cgcttgtcgc gcgagtcgta
     5701 gagctcttcg cggaggttac gcgcatcggt gaccccatcg gaaaagacgg caacgacgtc
     5761 gccaggatag agcttgacgt cgatctgctt gtaatcagac tcgggaacga tccccagcgg
     5821 aaagccggcg atctcctcac cgacttcatc gaccgtgccg ttgacacgtc gaaccatgac
     5881 ggggagatgg ccggcggagg tgatcgacag tgtgcgtttt tcggtgtcga gcacgctcaa
     5941 gctcagtgtg atgaattttt cctcaatggc cgccgagaaa agcaggctgt tgagctcgtt
     6001 cgaggcggcc gccggtgaat tctcggtgag gatgcaatag cgagtgtcac cggaaaactt
     6061 ggccatcatg agcgccgcgg caaccccttt gccggagacg tcgcccacgg cgacggcaac
     6121 ccggctgccg ggcaagggaa caaagtcgta gtagtcgccc cccacctcgt aggccggatc
     6181 gtaatgggcg aaaaactcgt aaccgggaat cgcgggcacc gattgtggca gaaatcgctt
     6241 ttgaacctgt tcggcgagct tgaggtctcg attcacgcgc tctcgctcaa gcaatgattc
     6301 gtgcatctgg gcgttttgaa tcgcgatggc ggcctggccg gccacggcgg caagcacatc
     6361 cagatcttct tggtggaact gcttgcggtc actcgtgtcg agctgaatga taccgagggc
     6421 ttcgcgatcg ggagtcaaga ggggaacgca catcaccgag cggatcttta gatcggcgat
     6481 cgacgccgag gtcggcagat tcttgtcggc gccagcatcc tggctcagaa ccgccttctt
     6541 ttggcccagc acatggttga cgatcgaccg gctgatgctc atcgggatct cgtcggcggg
     6601 gatgatcgtc gagaagatcg acttcttggc gggcttgtat ttgaaggcct tgcggatgag
     6661 tcgcttggtt tcagggtcga cgagcaccag gaagagccgc tccgcctgag ggaagagttc
     6721 catgagtgag tcgaggatct tgggcgcgac ggtgtcgatc ttgagctcgg tggaaaggtt
     6781 gcgggcaatc tcgaggattg ccttgagctt gacctccggc ttgatcatgc tagccatggc
     6841 gctcgaacgc gaggcgtcaa gcgtgtggag gtggggtgcc tcgaggtttt cgccctcggt
     6901 gacgatcatg atgtcgccag gttccttcga ggggctgtcc gccggcagct gcgggtaaaa
     6961 cagaaactcg acatcgcaga tattgatccg gtcacccggt gcaaggcggt gatcgattcc
     7021 cggaatgact ttg
//
LOCUS       DATAQL010000221         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3230144, whole genome shotgun sequence.
ACCESSION   DATAQL010000221 DATAQL010000000
VERSION     DATAQL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3230144"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VHS97_03795"
     CDS             complement(<1..496)
                     /locus_tag="VHS97_03795"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX3447345.1"
                     /translation="MSAGAWDARADDGIAPKAVYALTNEPTGNHVAVFARDGGGMLTP
                     VGMVPTGGAGSGENTHSQGGLAFSDDRQILYAVNSGDNTITVFALTPNGPVAVQRIES
                     GGQLPVSVAVRKDVVYVLNAGSVAGGVDNIAGFRVGPQGSLSPLPGSSRSLSAQMVGP
                     AQIAF"
     gene            complement(877..1233)
                     /locus_tag="VHS97_03800"
     CDS             complement(877..1233)
                     /locus_tag="VHS97_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447346.1"
                     /translation="MANWTRPGLRAGALAALSWLWASCERISLSESDSLDRDLSKFEL
                     LMVKAHQLTCPACRRFGRQVRLLDAALAGIRSRREAGDRLSGLFLPPDARERIKAALR
                     AANDSGGPVNSRAPTD"
     gene            complement(1247..1873)
                     /locus_tag="VHS97_03805"
     CDS             complement(1247..1873)
                     /locus_tag="VHS97_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447347.1"
                     /translation="MDDAAALDPETWVDQYGDSLFRFAVSRVQDPETASDLMQETFLA
                     ALRIRHTYAGRSSVRTWLVAILKHKIADHLRKIGREQRSREDMGPGDGTEGMFDRRGR
                     WRIPPLDWGSDPLREYERREFWEALGQCLSKIPAHLVDAFLECELEGSSREAICRDLN
                     VTPENLSVRLFRARLLLRRCLELNWFCSGEARSKGPTELTCPTAWTAP"
     gene            complement(2422..3615)
                     /locus_tag="VHS97_03810"
     CDS             complement(2422..3615)
                     /locus_tag="VHS97_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447348.1"
                     /translation="MPVWHEATREWVKQGRLVVLGVTQEQHADRCRLFAQWKQFEWPI
                     LHDPINLLEAPAVPIVVAIDEHGIVRAIRPRPATFKDDFLDKTFADDAPPGAPRSAGR
                     PDATSLRRKAEANPDAGGWRSVGDALTIWGGSEQLEQAIDAYARALALDPSDKNSLFR
                     LGVAHRMRHESASRRPGDFQAAIDAWGRALDLDPNQYIWRRRIEQYGPRLAKPYSFYD
                     WVVQAKADVSRRRETPVALAVEPYGSELAGPVRDVTAEASAPLEPDPKGQIHRDESRL
                     IEAEMVVVPRRVRPGQAARVHVTFRPGAAHSAHWNNESTPLRVWVAGADGWTIASRLL
                     EAPHGDQPESVEVRRLDFEVKAPPTAQGTTRLAAYALYNACEQAGGRCLFLRQDLSVE
                     LDVAK"
     gene            complement(3688..>3880)
                     /locus_tag="VHS97_03815"
     CDS             complement(3688..>3880)
                     /locus_tag="VHS97_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447349.1"
                     /translation="GRQLTPFSPNNRRRTMRKLALTLAACAILLASTSRAEAASYAPK
                     VGERHPDFTLPTIGDRTPT"
BASE COUNT          705 a         1231 c         1259 g          685 t
ORIGIN      
        1 tgaaagcaat ctgcgccgga cccaccatct gggcgctcaa ggaccgggat gatccgggaa
       61 gagggctgag ggaaccctga gggccgaccc ggaagccggc gatgttgtca accccgcccg
      121 cgacgctgcc ggcattgagc acgtagacaa cgtctttccg gacggccacg ctaacaggaa
      181 gctgcccgcc ggactcgatc cgctggaccg caaccgggcc attgggggtg agtgcgaaaa
      241 ccgtgatcgt gttgtcgcca gaattgaccg catacagaat ctgccggtcg tcgctgaagg
      301 cgagcccccc ctgactatgg gtgttttccc ccgatcccgc gccgcccgtg ggcaccatgc
      361 cgaccggtgt cagcatccca cctccgtccc gcgcgaagac ggccacgtgg ttgcccgtcg
      421 gttcgttggt gagagcataa actgctttcg gggcgatacc gtcgtcagcg cgtgcgtccc
      481 aggcgcccgc cgacatgacc accgccgcca aagcacctcg aagccatccg cgtctgctgc
      541 agcttgaatt ccccatggat agacctttct ccttttgtcc gagatcatgg ccgggcgtcc
      601 gcacacgcgg taccaaaccg gccagggatt agtcgcggac cggcgtgggg aattacacgg
      661 tttttcagcg gcactaaaga gacggtcact tcgtcattga tgagcaaggc gagaggctgt
      721 ccgctcgaag agggagcagc ccaccgtcga ccgaactacg gatgatctcg gctaaaaaac
      781 gaaggataat ttccgtcgac gtccttcgtc ccaccgtgcg ccggtctttc cagccgcctg
      841 ggccgtgaat cgattagata cgacggagct cgatcctcag tcggtcggtg ccctggagtt
      901 gaccgggccg ccgctgtcgt tggcggcccg cagggcggcc ttgatgcgtt cgcgagcatc
      961 tggcgggagg aacaggccgg agagcctgtc gcctgcctca cgccgtgacc tgatacctgc
     1021 cagcgctgca tcgagcaaac ggacctgcct cccgaaccgc cggcatgccg gacatgtgag
     1081 ttggtgggcc ttgaccatta gaagctcaaa tttcgacaga tcacggtcga gcgaatcgga
     1141 ttcagaaagg gaaatccttt cgcaagacgc ccagagccac gacaaggcag cgagggcccc
     1201 ggcacgaagc ccgggtcgcg tccagttggc catgagctac ctcccttcag ggggccgtcc
     1261 aggccgtcgg acaggtcaac tccgtaggac ctttggacct cgcctccccg ctacaaaacc
     1321 agttcagctc caggcagcgc ctcagaagca ggcgggctcg aaagagtcgg acggagaggt
     1381 tttctggggt tacgttcaga tctcgacaga tggcctcgcg gctcgatccc tcgagctcgc
     1441 attcgaggaa cgcgtcgacc aagtgagcgg gaatcttcga caagcattgt ccgagcgcct
     1501 cccaaaattc acgacgttcg tactcacgga gcgggtcgct accccagtcg agcggtggga
     1561 tgcgccagcg gccgcgacgg tcgaacatgc cttcggtccc gtccccgggc cccatgtctt
     1621 cccgagagcg ctgctcgcgg ccgatcttcc ggaggtggtc ggcgatctta tgtttgagga
     1681 tggcgacgag ccaggtgcgg accgacgacc gaccggcata cgtatggcgg atccgcaggg
     1741 ccgcgaggaa tgtctcctgc atcaggtcgg aggcggtctc cggatcctgc acccgcgaga
     1801 cggcgaaccg gaagaggctg tccccgtact gatcgaccca cgtttcgggg tcgagggccg
     1861 ctgcatcgtc catcgcttga agccactctg aaagcgtgtc ttgttcacat aatgaaaaat
     1921 tgatcttata gacgtccgtc tttcgattgt tattcgggcc ggacctagtg tctttccggt
     1981 tctgatctgt tagtcgcagg acggcgcatt gcattacaga caatgcacca cggactgcgg
     2041 aaacgcctgg tgtcggtgac cgccgacgag ggcacccggt ctctcggctt cgcacggcgg
     2101 gtgccaagcc ggcgatcctc ggaaatattt gtcgtaaccg ccgagcaaat gagtagcttg
     2161 ggacgatggt tccccttcgg gggaagcatg ggccgcgagt gatccgaggg gccacgccac
     2221 gaaatgacgt ggctcccaca aaatctcgtg ggcggatcgt gtgataccga gcttcttgag
     2281 cccgctgcgg agggtgtgga agtgtaaacc aggacgagat cggcgacgaa gaagctatct
     2341 attggcgccg gtcgccacgg agaacggccc tcgaccggcg gccaaagatc tgcttgtcct
     2401 catcgtgttc cagggccgcc ctcatttcgc cacgtcaagc tcgaccgaca ggtcctgacg
     2461 gaggaagagg caacgtcctc ccgcttgttc gcatgcgttg tagagggcgt aggccgcgag
     2521 gcgggtcgtt ccctgcgcgg tcggcggtgc cttcacctcg aagtcgaggc ggcggacctc
     2581 aaccgactcg ggctggtcgc cgtgcggcgc ctccagcagc cgcgaggcga tcgtccaccc
     2641 gtctgccccc gcgacccaga ctcgcagcgg cgtcgattcg ttgttccagt gcgcactgtg
     2701 ggccgccccg gggcggaagg tgacgtggac ccgagccgcc tgtccggggc ggacccgtct
     2761 cggcacgacg accatctcag cttcgatgag ccgcgattcg tcccggtgaa tctgcccctt
     2821 cggatcgggc tcaagtgggg ccgacgcctc agcagtgacg tcgcggacgg ggcctgcaag
     2881 ttctgacccg tacggctcga ccgcgagggc gaccggcgtc tcgcggcggc gagagacatc
     2941 cgccttagcc tgcacgaccc agtcgtagaa agagtacggc ttggccaggc gcgggccgta
     3001 ttgctcgatg cgtcgacgcc agatgtactg gttcgggtcg aggtccaggg cgcggcccca
     3061 ggcatcgatg gcggcctgga agtcaccagg ccttcgactc gccgactcat gtcgcatccg
     3121 gtgggcgacg ccgagacgga acagggaatt cttgtccgag ggatcaagag ctagggcgcg
     3181 ggcataagca tcgatcgcct gctcgagctg ctccgagccg ccccagattg tcagggcgtc
     3241 gccgaccgac cgccagccgc cggcgtccgg gttcgcctcg gccttgcggc gcagcgaggt
     3301 cgcgtccggg cgacctgccg accgtggagc acccggcggc gcgtcgtcgg cgaacgtctt
     3361 atcgaggaag tcgtccttga acgttgcggg ccgcgggcgg atggcgcgta cgatcccgtg
     3421 ctcgtcgatt gctacgacga tcggcacggc cggcgcctcc agaagattga tcgggtcgtg
     3481 gaggatcggc cactcgaact gcttccactg ggcaaacagc cggcagcggt cggcgtgctg
     3541 ctcctgagtg acgcccaaga ctaccaggcg cccctgcttg acccactcgc gagtcgcttc
     3601 gtgccaaact ggcacgtgct gtcggcagcc cactcaccag gaggcgaact ggatgagcag
     3661 caccttcttg ccgcggaaat ccgacaacta ggtcggtgtg cgatcgccga tcgttggcag
     3721 agtgaagtcg gggtgtctct cgccgacctt cggcgcatag gacgcggcct cagcgcgcga
     3781 cgtcgaggcg agcaagatgg cacaggccgc aagggtgagg gccaacttcc gcattgtccg
     3841 tctcctatta ttcggggaaa aaggggtcaa ttgacggcct
//
LOCUS       DATAQL010000222         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_939562, whole genome shotgun sequence.
ACCESSION   DATAQL010000222 DATAQL010000000
VERSION     DATAQL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939562"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VHS97_03820"
     CDS             complement(<1..288)
                     /locus_tag="VHS97_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3447350.1"
                     /translation="MTTPNKTRLDADTFRLTMLIFAIIAFMYFTGEVLKPLALSVLLS
                     FALAPAARILERCGLPRVAAVILTVVISLGLLGGIGYVVGQQLTSLAKRMPD"
     gene            complement(479..1813)
                     /locus_tag="VHS97_03825"
     CDS             complement(479..1813)
                     /locus_tag="VHS97_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3447351.1"
                     /translation="MPFMPFNILGIWFRGLLSLATIAGGIYLLTLWYHESHAVVPIRE
                     GPIADARGGNSEARDQDALGAVRTRSVFQFEPGMNRQTAYLATAIALLALSVVGGRVR
                     QAAATVFGGPEPARAEEAGEPFDDRSGEVHSLTRPDGSVLRVECYGPADAFPIVMTHG
                     WGCDSTEWFYSKKKLADRFRLVLWDEPGLGLSTKPANNNYRLEKLAGDLAAVLAFASD
                     RPAVLIGHSIGGMITLTLCKAFPELLGPRVAGLVLAHTSYKNPVRTTKWAALYTAIEK
                     PVITPLLYLTIGLWPLVWVMNWLSYLNGSAHRSTHKESFAGTETRGQLNFFARFMPRS
                     RPDVLARGMLGMIGYDAAAILRTIQVPTLVIVGDQDTTTLPEAGEFIRRSVPGAQLAT
                     ISRAKHLGLIEHNGRFNELVAEFAESRLAAGVALAVGPVKSVGPQVSRMDFD"
     gene            complement(1947..>2928)
                     /locus_tag="VHS97_03830"
     CDS             complement(1947..>2928)
                     /locus_tag="VHS97_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX3447352.1"
                     /translation="TWSGSLWQVWVLLFSGLDEPPRTIAGRLVTMAILVLGVGTAGLF
                     TASVASVLVERYLRRRDVSSFEMDDHLVLCNWSPRGMAWIHEVHAKSIQDKRPVVIIH
                     DAADEIELPDKKEDAAFDDVYIVKGDPTSEVVLRRASVPMAHSVVILADGREGKHADG
                     KSVLTCITIRSICRGERQPNVAVECHNSINRNHLRKAGADEIISSEELGLRLLARSAL
                     FHGMTRVYQELLTVGRDANEMYLLPAPEGLVGKDFVELTGMFARHRDDRKSCLLIGIQ
                     RGDEMHLNPIGGEAGPLHHDDQLIVLCRVFLQPSQTLPTDPPMVPPGGSK"
BASE COUNT          606 a          897 c          866 g          559 t
ORIGIN      
        1 atccggcatg cgtttggcga gcgaggtcaa ctgttggccg acgacgtagc cgattccacc
       61 caaaagtcca aggctgatta caaccgtcag aatgaccgct gcaacccgtg gcaagccgca
      121 ccgctcaaga atacgcgccg ccggcgccaa cgcgaaactt agaagcaccg acagcgcgag
      181 cggcttgagg acttcgcccg tgaagtacat gaaggcgata atcgcgaaga ttagcatcgt
      241 cagccggaag gtgtcggcat ccagacgggt cttgttcggg gtggtcactg actttttcct
      301 ccatggggcg cggccaaggc atcttgtgtc tgcgacggaa tatataaatc caaacggaaa
      361 acgtgcgtcg atcttagatg gccaaacccg gtgccgcggc gtggattagc acgcgcttcg
      421 cgtaagacgg gggacatgcg agttattaaa tactttgcgt ccgtcgcagg tttgacgatt
      481 aatcgaagtc catgcgcgac acctgtggac cgactgactt gactggaccc acggcgagcg
      541 cgacgcccgc agcgaggcgc gactcggcga actcggcgac cagctcgtta aatcgtccgt
      601 tgtgctcgat caaaccaagg tgcttggccc gggagatagt cgcgagctgg gcgccgggca
      661 cgctacggcg aatgaattcg ccggcttccg ggagcgtcgt ggtatcctgg tcgccgacga
      721 tgacgagggt aggaacctga atcgtccgca gtatcgccgc cgcgtcatag ccgatcattc
      781 ctagcattcc ccgagccagg acgtcggggc gactgcgtgg catgaatcgg gcaaaaaaat
      841 tgagctggcc ccgcgtctcg gttccagcga acgactcctt gtgcgtcgac cggtgcgccg
      901 aaccgttgag gtaactcagc cagttcatca cccacacgag cggccagagc ccgatcgtca
      961 ggtaaagcaa cggggtgatg accggcttct cgatcgcggt gtaaagcgca gcccatttcg
     1021 tcgtccggac agggttctta tagctcgtgt gggcgagaac gagcccggcc acacgcggac
     1081 ccaatagctc gggaaatgcc ttgcagaggg tcagtgtgat cattccgccg atgctgtgcc
     1141 cgattagaac ggctggccgg tcgcttgcga acgcgagcac ggccgcaagg tcgcccgcta
     1201 gcttctccag tcggtagttg ttattggccg gcttcgtcga gaggcccagc cccggttcgt
     1261 cccaaaggac caggcggaag cggtcggcga gttttttctt ggagtaaaac cactcggtgc
     1321 tatcgcaccc ccagccgtga gtcatcacga tcgggaacgc gtcggcgggg ccgtaacact
     1381 cgacccggag cacgcttccg tcggggcgcg ttagcgaatg aacttcaccg ctccgatcgt
     1441 caaaaggctc gccagcctcc tccgcccgtg cgggctctgg acctccaaat accgtcgcgg
     1501 cggcttgccg gacacggccc cctacgacag ataatgccag caaagcgatc gccgtcgcga
     1561 ggtatgcggt ctgccgattc atccccggtt cgaattgaaa aacgctgcgc gttcggacgg
     1621 cgccgagggc gtcctggtca cgcgcttcgc tgttccctcc ccgcgcgtcc gcaatggggc
     1681 cttcccggat cggtacgact gcgtgcgact catggtacca cagtgtcaag aggtagatgc
     1741 cgcccgcgat agtcgctagg gacagtaggc cacggaacca gatcccaagg atgttgaacg
     1801 gcataaaagg cattactgcg atctccagtc ggacactggc gcggacggac ggatcccggt
     1861 taatatcgct ggcgcgacac tatcaattca tctcacaatg caaaacataa gccgtctttg
     1921 gactagtttg cgttcatggt tcggaatcat ttcgatccac ctgggggcac catcggcgga
     1981 tcggtcggca aagtctggga aggctggaga aatacgcggc acaagacgat gagttgatcg
     2041 tcgtgatgca gcggacccgc ctcgccgccg atggggttga ggtgcatttc gtcgccccgt
     2101 tggatgccga tcagcaggca cgacttgcgg tcgtcacgat gccgcgcgaa catcccagtg
     2161 agctcgacaa agtctttgcc cacgagtcct tctggtgccg gcaacaaata catctcgttc
     2221 gcatcgcggc ccacggtcaa aagctcttgg tagacgcgcg tcattccgtg gaagagtgcc
     2281 gaacgggcca atagtctgag gcccagctcc tccgaagaga tgatctcgtc ggccccagcc
     2341 ttccgcaggt gattgcgatt aattgaattg tggcattcga ccgcaacgtt cggctgccgc
     2401 tcgccccggc agatactgcg gatggtgatg caggtcagga cactcttccc atcggcatgc
     2461 ttaccttccc gtccatcggc gaggatcacg accgagtggg ccatcggcac gctggccctg
     2521 cgcagcacga cttcacttgt cggatcaccc ttcacgatgt aaacgtcgtc gaacgcagca
     2581 tcttcctttt tgtccggcaa ctcaatctcg tccgccgcat catgaatgat cacgaccggc
     2641 cgcttgtcct ggatcgattt ggcgtgaact tcatgaatcc acgccatccc gcgaggggac
     2701 cagttgcaca ggacaagatg gtcgtccatc tcgaaactcg acacgtcacg cctccgtaga
     2761 taacgttcga ccaatacgga cgcgacgctg gccgtaaaaa gtcccgccgt gccgacaccc
     2821 agcacgagga ttgccatggt caccagccgt ccggcaatcg tcctcggcgg ctcgtcgagc
     2881 ccgctgaaaa gcaggaccca tacctgccag agtgatccgc tccaagtc
//
LOCUS       DATAQL010000223         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2619156, whole genome shotgun sequence.
ACCESSION   DATAQL010000223 DATAQL010000000
VERSION     DATAQL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619156"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VHS97_03835"
     CDS             complement(<1..419)
                     /locus_tag="VHS97_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX3447353.1"
                     /translation="MASFRFRLAVYGMTVLAVAKVVACAPLTAAEPDTPDAFQRQVMP
                     LFERYCVDCHMNGESEGGIALDRFANQEAAIKDGKIWIRVRDAIAERIMPPADEPQPS
                     IDELGRIVAWIEKDFLAAQCAKGESSAPVVLRRLNRR"
     gene            complement(598..>3455)
                     /locus_tag="VHS97_03840"
     CDS             complement(598..>3455)
                     /locus_tag="VHS97_03840"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEX3447354.1"
                     /translation="DVFVEKFIRNAKHIEVQILGDKGGNLVHLYERDCSVQRRHQKIV
                     EIAPAPSLDPVVRSAICEAAVELVRHVQYDNAGTVEFLVDISTGSFYFIEVNPRIQVE
                     HTVTEIVTNIDLVKSQILVADGKPLSDPEIDLATQESVQVQGYALQCRVTTEDPENRF
                     TPDYGRITHYRPVGGLGIRTDGGPAVTGGIITPFYDSLLVKICASGRRFVDACRRMER
                     ALQEFRVRGVKTNVPFLLNLLEHPDFLAGNCTTRFIDETPELFRFPVRQNRAMRLLTY
                     AAEITVNGFPGVARPAGHAAAPEPDPPRLDSTAGPAPGFRQRFLVMGPDPFSRWVRDY
                     QPLLVTDTTFRDAHQSLLATRVRTRDMLRVADAYARMCPGIFSLEMWGGATFDTSMRF
                     LKEDPWERLAQLREKIPNILFQMLLRGSNAVGYTSYPDNVVKAFVKEAAAAGIDLFRV
                     FDSLNWVPNMELSLETVREVGGLCEASICYTGDILDPGRPKYNLAYYVGLAKELEKRG
                     ANLIAIKDMAGLCKPMAAERLIKTLREEVGVPIHFHTHDIGGAQAASVLRGADVGLDI
                     ADGAVASMAGLTSQPSLNALVESLRFSPRDTGVDLAALIEMSRYWEEVRALYNPFESG
                     PRSPAADLYQLEMPGGQYTNLYQQAKALGLAARWLEVCKAYSQVNQLFGDIIKVTPSS
                     KVVGDMALFVVANNLTPDDILDPARELAFPESVVEFFEGRLGQPPGGFPPALQARVLK
                     GRPPLNVRPGANLPPADVDGARDKATALLGRPASDRDALSYLLYPRVFPDLAAHERLY
                     SDTSILPTSIFFFGPDPGAEHLVEIEPGKTLIVKLLAVGEPHADGNRTVFFELNGQPR
                     EVEVADRSLASSVRETPMADPADPNQIAAPLPGLVVGVAVSAGDAVRKGQKLLSIEAM
                     KMETTIYAERPGRVTDMLATVGRQVKTGELLLKFGPQ"
BASE COUNT          661 a         1067 c         1074 g          653 t
ORIGIN      
        1 tcgcgccgat tgagccggcg caagacgaca ggtgccgagc tctcgccctt cgcacactga
       61 gcggccagga agtctttctc gatccatgcc acaattctgc cgagctcatc gattgatggc
      121 tgaggctcgt ccgctggcgg catgatgcgt tctgcaatgg catctcgcac tcggatccag
      181 atcttgccat ccttgatggc agcctcttga ttggcaaagc gatcgagagc gatgccccct
      241 tctgactctc cgttcatatg gcagtcgacg cagtagcgct caaaaagcgg catgacctgc
      301 cgctggaacg cgtcgggtgt gtccggctcg gctgcggtta agggcgcgca ggccaccacc
      361 tttgctaccg ccaggaccgt cattccgtat acagccaacc tgaacctgaa acttgccatt
      421 attttgtgtg actcgtttcg attgtgtgga cacccgtttc cccaatcggt cactgacggg
      481 ggatttctta gacaattatt gaacaccaat cgaccgatcg ggtctagtga ataatgtcat
      541 aagcgtcaaa aggcgtcctg atgccttgta ttaacctgtt ttaaggatcc tgaatcttta
      601 ttgcgggcca aatttcagca ggagctcccc ggtctttacc tgccgcccga cagtcgccag
      661 catgtcggtc acgcgaccgg ggcgttcggc atagatcgtc gtttccatct tcatggcttc
      721 gatcgacagc aatttctgtc ctttgcgcac agcgtcgcca gccgagaccg ccacgccgac
      781 caccaggccc ggcaacgggg ccgcaatctg gttgggatcg gctggatcgg ccatcggggt
      841 ctcgcgcact gacgaggcaa gagaacgatc ggccacctca acttcccgcg gctggccgtt
      901 aagctcgaaa aagaccgtcc ggttgccgtc ggcgtgcggc tcgccgacgg ccagcagctt
      961 gacgatcaac gtcttgcccg gctcgatctc caccagatgc tcggccccgg ggtcgggacc
     1021 gaaaaagaag atcgacgtgg gcaggatcga cgtgtcggaa taaagtcgct cgtgagctgc
     1081 cagatccgga aagacgcgcg gatagagcag gtaactgagc gcatcgcggt cgctcgccgg
     1141 acgtcccaaa agcgcggtcg ctttgtcacg ggcaccatca acgtcggccg gcggtagatt
     1201 ggcgcccggc cgcacgttca aagggggacg tcccttcagc acgcgcgcct gaagcgcggg
     1261 cggaaagccc cccggcggtt ggccgagccg gccctcgaaa aactcgacga cactttcggg
     1321 aaatgccaac tcgcgcgcag ggtcaagaat gtcgtcgggc gtcaggttgt tcgcgaccac
     1381 aaagagcgcc atgtcgccga cgaccttcga gctcggcgtt accttgatga tgtcgccaaa
     1441 gagctgattg acctgggaat aggccttgca gacttcgagc caccgcgcgg ccaggccgag
     1501 tgccttggcc tgctgataca ggttggtgta ctgcccaccc ggcatctcga gctggtagag
     1561 gtcagcggct ggcgagcgcg ggccggactc gaacgggtta taaagcgccc gcacttcctc
     1621 ccagtaccgg ctcatctcga tcaaggcggc cagatcgacc cccgtgtcgc ggggtgaaaa
     1681 ccgtagagac tcgacgagcg cgttcaagct gggttgcgag gtcaacccgg ccatcgaggc
     1741 gactgcgcca tcggcgatgt cgagcccgac gtccgccccg cgcagcacac ttgcagcttg
     1801 cgcacccccg atgtcgtgcg tgtggaagtg gatcggtacg ccgacctcct cacgaagggt
     1861 cttgatcaac cgctcggcgg ccatgggctt acataacccc gccatgtcct tgatggcgat
     1921 caagttggcc ccgcgttttt cgagctcctt ggccaggccg acataatacg ccaggttgta
     1981 cttgggacgg cccgggtcga ggatgtcgcc ggtatagcag atactggctt cgcacagccc
     2041 gccgacctct cggacggtct cgagcgacag ctccatattg gggacccagt tgagcgagtc
     2101 gaacacccgg aacagatcga ttccggccgc ggcggcctct ttgacgaacg ccttgaccac
     2161 gttgtcgggg tagctggtgt acccgaccgc gttcgacccg cggagcagca tctggaacag
     2221 aatgttgggg atcttctcgc gaagctgggc cagccgttcc caggggtctt ccttcaagaa
     2281 ccgcatcgat gtgtcgaagg tcgcgcctcc ccacatctcg agcgaaaaga tccccggaca
     2341 catacgtgcg taggcgtcgg ctacgcgcag catgtcgcgt gtgcgcacgc gcgtcgccaa
     2401 gagcgattga tgagcgtcgc gaaacgtcgt gtctgtgacc aagagcggct gatagtcgcg
     2461 cacccagcgt gaaaaggggt caggacccat tacgaggaac cgctggcgga agccaggcgc
     2521 cggcccagcc gtcgagtcga ggcggggcgg atcgggctcg ggcgcagcgg catgtccggc
     2581 gggccgcgcg acgccgggaa acccgttaac cgtgatctcg gcggcatagg tcaagagccg
     2641 catcgcccgg ttctgccgga ctggaaagcg gaagagctcg ggggtctcgt cgatgaaccg
     2701 cgtcgtgcag ttgccggcaa ggaaatctgg atgctcgagc agattgagca ggaaggggac
     2761 gttggtcttc acgccgcgca cgcggaattc ttgcagcgct cgctccatcc gccggcaggc
     2821 gtcgacgaac cgtcggcccg aggcgcagat cttcacgagc aatgagtcgt aaaacggagt
     2881 gatgatcccg ccggtaaccg ccgggccgcc gtcggtccgg atccccaatc cacccaccgg
     2941 ccgatagtgg gtgatccggc cgtagtccgg cgtgaatcgg ttttcggggt cctcggtcgt
     3001 cacacggcat tgcagtgcat agccctgcac ctgaaccgac tcttgcgtgg ccaagtcgat
     3061 ctccgggtcc gagagcggct tgccgtcggc gaccaagatc tggctcttga ccaggtcgat
     3121 attggtgacg atctcggtga cagtgtgctc gacctggatg cgcggattga cttcgatgaa
     3181 gtaaaacgac ccggtgctga tgtcgaccaa gaactcaaca gtccccgcgt tatcgtactg
     3241 aacgtggcgg acgagctcga ccgccgcctc gcagattgcc gagcgcacga ccgggtccag
     3301 gctaggtgcc ggtgcgatct cgacgatctt ttggtgccgg cgctgaacgg aacaatcgcg
     3361 ctcgtacaga tgcaccaggt tgccgccttt gtcgcccagg atctgcacct cgatgtgctt
     3421 ggcgttgcgg ataaactttt cgacgaacac gtcgg
//
LOCUS       DATAQL010000224         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4297750, whole genome shotgun sequence.
ACCESSION   DATAQL010000224 DATAQL010000000
VERSION     DATAQL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4297750"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..646
                     /locus_tag="VHS97_03845"
     CDS             212..646
                     /locus_tag="VHS97_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX3447355.1"
                     /translation="MSWASRLADLTVFFHATYVGFIVFGLAAILLGVVFRWNWVRNIW
                     FRAIHLIAIGIVVGESLVGMPCPLSIWEAQLRKAAGQTVYSGDFLGHWAHRLIFFRAD
                     PWVFTLIYMLFGMAVLATFLLAPPRRSRRADRSAPGTVPGSR"
     gene            721..1959
                     /locus_tag="VHS97_03850"
     CDS             721..1959
                     /locus_tag="VHS97_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEX3447356.1"
                     /translation="MMSSLARYRLGLILILATLVMGLVTVIGPRPATAETCLSPYVKR
                     LDRPEKYLYLFCVDADAQDNDFVTVIDVNPDSLTCGTILNTVDLGSKGNETHHWGYTD
                     DRTRIWAGGLFSNRIWILDVATDPAKPRIEKVIDNVTASSGLSGPHSYYALPGRMLIA
                     FLGAGDGGLPAGLAEFTNDGKFIRRLENPSEAPYGYDVAVKPSVNRMVTSSFTPFRNY
                     RKPLAQMDLKDFGRDLIVWDFKNRQPVQVGKTGLAPLEVRWSLKPENNYGFTNCALDN
                     SIWMFKGNGEGNYEFKKVADTGAMPADLRQSPDDKYLYVSCFGDNLIQQWDVSVPEHP
                     QLKSTVAPCVQPNMMHVTGDGKRMYVSNSLLSTLDHSGRFCVRLVHVGPDGLKVDPFF
                     NIDLNKFPTGPARGHDMLIN"
BASE COUNT          423 a          631 c          637 g          450 t
ORIGIN      
        1 cgctcgcgaa atttgccttt cggaaccaaa ccattgccgc acttgcagtt gcgcatgtcg
       61 ccggaaatcg ggacgatttg ttagccgcaa tttggcgtat catgatttgt tgtataagcg
      121 gaccacagcc tcgtatttgt ccggctcctg agtcggtatt atcacgagag gtcgatcctg
      181 tgccaccgat ccacctcggg ggaggccttc catgtcttgg gcgagcagac tggcagacct
      241 gaccgtcttc ttccacgcga cctacgtcgg tttcattgtg ttcggcttgg cggccattct
      301 gctcggcgtt gtgttccggt ggaactgggt ccgcaacatc tggttccgcg cgatccacct
      361 gatcgcgatc gggatcgtcg tcggcgagtc tctcgtgggt atgccgtgcc cattaagcat
      421 ctgggaagcc cagctcagaa aggcggccgg gcagacggtg tactcgggcg atttcctcgg
      481 ccactgggcc caccggttga tcttcttccg cgcggacccc tgggtattca cgctcattta
      541 tatgctcttc ggcatggctg tcttggcgac gtttttgctg gcaccgccac gacgttcgcg
      601 gcgcgccgat cgatctgctc ccggcaccgt gccgggatct cgataaaatt gatcgaaatt
      661 gtataatcga tgttgctggc gcgagatccg gcgcaagtcc ccagagtgag gaggaaagca
      721 atgatgagca gcttagctcg atatcggctg ggtttgatac tgatcttggc gactctggtg
      781 atgggactgg tcacggtcat cgggccgcgg ccggcgacgg cggaaacctg cctgtctcct
      841 tatgtgaagc gattggatcg gccggagaaa tatctgtacc tattctgtgt ggatgccgac
      901 gcccaggaca acgacttcgt caccgttatc gatgtgaacc ccgacagcct gacctgcggc
      961 acgatcctga acacggtcga cctgggctca aaaggcaacg agacccatca ctggggctac
     1021 accgacgacc gcaccagaat ctgggcaggc ggattgtttt ccaatcggat ctggatcctg
     1081 gacgtggcga cggacccggc caagccgcga atcgagaagg tgatcgacaa cgtcaccgcg
     1141 tcgagcgggc tgtcgggtcc ccacagctat tacgcactgc cgggccggat gctgatcgcc
     1201 tttctggggg cgggagacgg tggtttgccg gccggtttgg ccgagttcac caacgacggc
     1261 aaattcatca ggcgcctgga aaaccccagc gaagccccct atggctacga cgttgcggtc
     1321 aaaccatcgg tcaatcgcat ggtcaccagc agtttcacgc cgtttcgaaa ctaccggaag
     1381 ccgctcgccc agatggacct gaaagacttc ggccgcgact tgatcgtgtg ggatttcaaa
     1441 aaccgccagc ccgttcaggt gggcaagacg ggactggcac ctctggaagt acgctggagc
     1501 ctgaaaccgg aaaacaacta cggattcacc aactgcgccc tagacaactc gatctggatg
     1561 ttcaagggaa acggcgaggg taactacgag ttcaagaagg tcgccgacac cggcgccatg
     1621 cccgccgacc tccgccagag ccccgacgac aaatacctat acgtgagttg cttcggcgac
     1681 aatttgattc agcagtggga tgtgtcggtc cccgagcacc ctcagttgaa gagtacggtc
     1741 gccccttgcg ttcagcccaa tatgatgcac gtgaccggcg acggcaagcg gatgtacgtc
     1801 tcgaactcgt tgctttcgac gctcgaccat tcaggccggt tttgcgttcg gttggttcac
     1861 gtcggccctg acggtttgaa agtcgacccg ttctttaata tcgatttgaa taagtttcca
     1921 accgggccgg cccgcgggca cgacatgctg attaactgag ccgttcgtga acaagccgga
     1981 cgactttgtc gagcgcgggt ctcctggccc cgcccgctgg ttgaccgaag gtctgccatg
     2041 gccagagggt gcatcgcata cggcaaaccc ttccaagcca aggcccacaa tgagcgccga
     2101 agacctccgg tcggccctgc ggcggggtcg ggagagccgc g
//
LOCUS       DATAQL010000225         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3381568, whole genome shotgun sequence.
ACCESSION   DATAQL010000225 DATAQL010000000
VERSION     DATAQL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3381568"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..>2041)
                     /locus_tag="VHS97_03855"
     CDS             complement(281..>2041)
                     /locus_tag="VHS97_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX3447357.1"
                     /translation="AAYSVYVQTNQLLISLSSEVERIPQVLRQLKRYPRWTYDGRLRE
                     LAKKSDVELRSQLGYRMALLEDDPGQVDGLFHRLLASDLAEMAVIRDSLLPYRAQISD
                     TYRAQLRDALLRSTDPRAFLILFSLIDEHNTAILADLRQAVDEPIVAADSETVKDAIA
                     ARRARAAVALLRLGQANQVWHRLEHSPDPRSRSAFINAINPLGADPALLADELEEIVK
                     NSSIGPINDGAVEDDKNAYLFDRKTSRSRGLILALAAYPPDQLEATRRDKLIGLLADL
                     YRNDPDAGVHAAAELVLVRLGRGELPQLEIGLPRPDAVARHRWYVSKAGHTMVRIEGP
                     VAFTMGSPFSEPAHQKHEVMHRRIIPRRFALASREVTVDQFQRFAIERRGSPHEFSKY
                     YSPEGDGPLISLSWFDAVQYCNWLSDQEGLPRCYLPNDRGEYADGMRIDHEAVAKGGY
                     RLPTDAEWEYACRAGTTTSRYYGDAPDLLGLYEWYIATSGFHARPCGRLLPNDLGLFD
                     MLGNVSEWCHDQHPEPLPHPGELVRDAITAEVVTGEKRNVRGETYAMIPAGLGSAVRT
                     WYDPTEYRADIGFRPARTLP"
BASE COUNT          402 a          609 c          622 g          408 t
ORIGIN      
        1 gggtgaggcg tgagatcgac gtccggaggt gccggctgat ctttcttcgg atcgggggat
       61 ttagccatgt cttcccatcc gccgcattcg cgtcttgaaa gccgatcaaa cccaacgaca
      121 acgacacatt actgagcttc aggactatac aggcatgaga tcgtcgtagc aatgatgtat
      181 tgccagcggt tttttttccg ataaacgggc cgccgacacg cgaccaaggg ctccgaaacc
      241 gtggctatgg atttcggagt cggcacaaga gcacgcgtgg tcaaggaagc gtgcgcgctg
      301 gtcggaaacc gatgtcggct ctgtattccg tcgggtcgta ccaggtgcgc acggcggacc
      361 ccagtcccgc gggaatcatc gcgtatgtct cgccgcggac attgcgtttt tcgccagtca
      421 cgacctccgc cgtaattgca tcgcggacga gttcgccagg atgcggtagg ggttccggat
      481 gttggtcgtg gcaccattcg ctgacgtttc ccagcatgtc gaacaagccc agatcattgg
      541 gcagcagccg gccgcaaggg cgggcatgga atcccgacgt ggcgatgtac cattcgtaga
      601 gaccgaggag gtcaggcgca tcgccatagt aacggcttgt cgtggttcct gcacggcacg
      661 cgtactccca ttcggcatca gtcggcagtc ggtatccccc cttggcaacc gcctcgtgat
      721 cgattcgcat gccgtcggcg tattcgccgc gatcgttggg gagatagcac ctgggcaatc
      781 cttcctggtc gctcagccag ttgcaatact ggaccgcgtc aaaccagctc aaagagatca
      841 ggggaccgtc gccttccggg ctgtagtact tactaaattc gtgaggtgat cctcgtctct
      901 ctatcgcaaa tctctggaat tgatcgacag tcacctctcg ggaagcgagc gcgaagcggc
      961 gggggatgat ccgccggtgc ataacctcgt gtttctggtg cgcgggttcg gagaacggcg
     1021 agcccatcgt gaacgcgacg gggccctcga tccgcaccat ggtatggccg gccttgctca
     1081 cataccagcg atggcgggcc acggcatccg gccgcggaag accgatctcg agctgcggaa
     1141 gttcgccgcg cccaagccgc acgaggacta actccgccgc cgcgtgaacc ccggcatcgg
     1201 gatcgttgcg ataaagatct gcgagcaaac cgattagctt gtcgcgcctt gtcgcttcaa
     1261 gctgatccgg cggataggcc gcgagcgcaa ggatgagtcc ccgactccgc gatgtttttc
     1321 ggtcgaagag atatgcgttc ttgtcgtcct caaccgcccc gtcgttgatc gggcctatgg
     1381 aactgttttt gacgatttct tccagctcat cggccaggag cgccgggtcg gctccgagtg
     1441 gattgatggc attgatgaag gcactccgcg accgcggatc cgggctgtgc tcgagtcgat
     1501 gccacacctg gtttgcctga cccagacgca agagcgccac cgcggccctt gctcgacgag
     1561 ccgcgatcgc gtccttcacc gtctcactat ccgcggcaac aatcggctcg tcgacggcct
     1621 ggcgcagatc agccagaata gccgtgttgt gctcatctat cagcgaaaaa aggatcaaaa
     1681 acgcgcgcgg gtcagtgctc cgcagcaggg cgtccctgag ctgtgcacga taagtgtcgg
     1741 aaatctgggc ccggtagggc aagaggctgt cgcggatgac agccatctcg gcaaggtcac
     1801 tggcaagcag gcggtgaaac aggccatcaa cctggccggg gtcgtcttcc aggagcgcca
     1861 tcctgtagcc gagctgcgaa cgcagctcga catccgattt cttcgccaac tcacgcaggc
     1921 gtccgtcata agtccagcgc ggataacgct tgagctggcg tagaacctgc ggaattcgtt
     1981 cgacctcgct cgaaagcgaa atcaacagct gattggtctg gacgtacacg ctgtaagcgg
     2041 c
//
LOCUS       DATAQL010000226         6424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_634432, whole genome shotgun sequence.
ACCESSION   DATAQL010000226 DATAQL010000000
VERSION     DATAQL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634432"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VHS97_03860"
     CDS             complement(<1..296)
                     /locus_tag="VHS97_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3447358.1"
                     /translation="MNTDLQSITGHLCLTGAGLSCLVLLALPIMAAVGPVRTGEQIYR
                     QQCAACHGSIGEGTDDHYPRPLMGERSVASLSRLIAKTMPEDAPGECVGEDADKV"
     gene            complement(645..1487)
                     /locus_tag="VHS97_03865"
     CDS             complement(645..1487)
                     /locus_tag="VHS97_03865"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3447359.1"
                     /translation="MTSQTQQTGAAEERHESIHRPEDGKVTVVVPGVELCPLVGSQCG
                     ARNLFTGLLTLGAKASYPLYTRPFSEALVLVEGEASVDVEDRSYCLKPFDAMTVSPRV
                     PRRVVNRSSSRPAILHVSLASSTPDQTWVNGRFVAVEQPTDVTGQAGTERLCRNSTVA
                     RLELAPLARFQDLFGVDLGARGICGGCGHFEPGARLPCHRHDFDESITIVQGTATCIV
                     EGRRHELSGNATMLVPEGRCHYFINLTLEPMAMIWVYAGDRPDRIVVDEAYCHPERAK
                     GSRA"
     gene            2075..2446
                     /locus_tag="VHS97_03870"
     CDS             2075..2446
                     /locus_tag="VHS97_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447360.1"
                     /translation="MKVGSVSKKATALKKAAPTKAKPSPVSKKATPALASKKVAIKKA
                     APKKAAGPKLTDPQQNMLKKVVAHQGSLGYHPAKVENKVLETLLKHKLVKKGKKHPET
                     KLFHYQVSTIGKKHLEMSAKS"
     gene            complement(2643..3539)
                     /locus_tag="VHS97_03875"
     CDS             complement(2643..3539)
                     /locus_tag="VHS97_03875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3447361.1"
                     /translation="MLTTSTHTSISTPTYPPIPHRLLGKTGCEVSQFGLGGEGVLRTH
                     GRMAEAVAVIHRALDQGVTYCDTSPAYAGSIDYYGAALGERRNQVFLASKTHDRTRDG
                     SLRLLDESLKRLRTNHLDLWQLHDLRTPSDLNMIFGRRGALEALVQARDEGRVRFLGV
                     TGHHDPAILLEAMRQFDFDTVLVALNAADVHRLPFAQTALVEAARRGMGVIGMKVYAA
                     GTLLRGDARSLPVASSEAMGYVLSLAGVSTVVIGCSSPAEVDENARIAREFQPFDAPT
                     MRTIEERTMARAGIFTSYKKLA"
     gene            complement(3563..4762)
                     /locus_tag="VHS97_03880"
     CDS             complement(3563..4762)
                     /locus_tag="VHS97_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX3447362.1"
                     /translation="MSIEPVLTQHKVTVLLVDDQPMIGEAVRRMLAGEPDIVFHYNRD
                     ARKALDEADRLKPTVILQDLVMPDIDGLTLVKAFRAHEPTKETPLIVLSTKEEPTVKA
                     EAFALGANDYIVKLPDRLELLARIRYHSKGYINLLQRNEAYGALLESQQRLANEMKQA
                     AHYVQSLLPDKLRKGDVRTDWRFVPSAELGGDSFGYHWLDDDHFAFYLLDVSGHGVGS
                     ALLSVSAMNALRSQALPQTDFRAPGQVLGALNNAFQMDQQNGLFFTIWYGVYHKPTRR
                     IDYAGGGHPPVLLFNGQSADQAALEILEAGGPMIGAVPDLEFKTATTSLGSFAKLFLY
                     SDGVYEIERTDKSMWPFNEFIEFMKKGPHDIAGDSKMDRLIAYDREIQGREEFVDDCS
                     IVELQFV"
     gene            complement(4885..5925)
                     /gene="cheB"
                     /locus_tag="VHS97_03885"
     CDS             complement(4885..5925)
                     /gene="cheB"
                     /locus_tag="VHS97_03885"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEX3447363.1"
                     /translation="MRIGIVNDIGLAREALRRVVQSCSQHEIAWTASDGAEAIALARE
                     DRPDLILMDLFMPGTDGVEATRRIMGESPCAILVVTATISGHLSKVYQAMGYGALDAL
                     DTPILGLHGQISGASLLLHKIEIIGKLLGNTAERHGDRRQAADSLIHTLHPLNSAELI
                     EPLVILGASTGGPNALAQVLAEIPATLEVPIIIVQHVDAAFAPGLGQWLSEQSHRHVT
                     LITEGHRPAARDVLLSGTDDHLILGEDRCLHYSAEPSDVSYRPSVDVFFASVARNWPK
                     PGVAALLTGMGRDGAAGLLKLRGQDWRTIAQDESSSVVWGMPKAAAEIGAAEQVLPIS
                     RVADAIVRLVPK"
     gene            complement(6060..>6424)
                     /locus_tag="VHS97_03890"
     CDS             complement(6060..>6424)
                     /locus_tag="VHS97_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3447364.1"
                     /translation="VVDDSITVRELERQLLENRGYTVDVAVDGVDGWNAVRSTHYDLV
                     VSDIDMPRMDGIQLVQHIKEDARLKAIPVVIVSYKDREEDRIRGLDAGANFYLTKSSF
                     HDRTFLTTVADLIGEPRQ"
BASE COUNT         1440 a         1846 c         1832 g         1306 t
ORIGIN      
        1 actttatcgg catcctcacc cacgcactcc ccaggggcat cttcaggcat ggtcttggcg
       61 attaaccgcg atagactggc caccgatcgc tctcccatga gtggccgcgg ataatgatcg
      121 tccgtgcctt cgcctatcga gccgtgacag gcggcacatt gctgacggta gatttgctca
      181 ccagttctaa cgggacccac agccgccatg atgggtaaag ccaggagcac gagacacgac
      241 aatccagccc cggtaaggca gaggtgaccg gtgatcgatt gcagatcggt attcatggga
      301 aagccaacca tcagggcctt ggactttagc gtgccaatac caggttcgac actcggccag
      361 gcgagattaa ccgctagctc cgggccaatg agacccagaa tccgttagcg gcaacgtcag
      421 gcaggaaccg ttctctcaag tggggtggta gtgagccggc cagcgttggc caatcagtcg
      481 cttcacgtca gaaagcacgc ggttcaacca ttggatcctt ttaacgctgg gattcaacaa
      541 accttttaca attttacctg attccgtgga tgtcagcaag tcaaccactg attgcggccc
      601 gtgattgctc gcgccgagtt gcttccacgc ccaacgcaag gacgttaggc tcggcttccc
      661 ttggcccgct cgggatggca gtatgcctcg tccacgacga tgcggtcagg ccggtcgccg
      721 gcatagaccc atatcatggc catgggctcc aacgtgaggt tgataaagta atggcagcgg
      781 ccttcgggga cgagcatcgt agcgttgcct gaaagttcgt ggcgccgccc ctctacaatg
      841 caggttgccg ttccctgcac gatggtgatc gactcgtcga aatcgtggcg atggcagggc
      901 aggcgcgcgc cgggctcaaa gtgcccacag ccgccacaaa tcccccgcgc gccgagatcg
      961 acgccaaaaa ggtcttggaa tcgcgctagt ggcgccagct ccaatcgcgc gaccgtgctg
     1021 ttgcggcaca gcctttcggt ccctgcctga ccggtcacat ccgtgggctg ctcaaccgcg
     1081 acgaaccggc cgttgaccca ggtttggtcc ggcgtcgatg aggcaagcga gacgtgaagg
     1141 atagcaggcc gtgacgatga gcgattgaca actcgacggg gcacgcgcgg tgaaacggtc
     1201 atcgcgtcaa aaggcttgag gcaataactc cgatcctcaa cgtcgaccga tgcctcgcct
     1261 tcaaccagta ccagcgcctc agagaacggc cgggtataga gcggatacga agccttggcg
     1321 cccaacgtca agaggcccgt gaataggttg cgcgctccgc actgagaacc caccagcggg
     1381 catagctcta ccccggggac taccaccgtg accttgccgt cctcggggcg gtgaatcgac
     1441 tcgtggcgct cctcggcagc cccggtttgt tgggtttgag atgtcatgac gcagaccaaa
     1501 ctcgaaagat ccaagacgta caaatccacg gctaactgtg gcgaagaggc gaccaaatcg
     1561 aaatctagcg cgagtctaac tcgtccgaga cgtacgatca actcggctgc atagactttg
     1621 gagggtcgcc gcgaaaagac gataagagca cagcatacaa catctatgtt aatatatgtt
     1681 aagttacagt aatagagtat aatattatat attttgacta tgaatattaa tcgttctgag
     1741 caggctttgt tgctttcccg tcggcattgg ccgaacacac ccgtgctctc ttgaacggcg
     1801 attggggtgt cggcaattta gttcatttgt tattcgtatc agtttacttg gcgcgtgtac
     1861 aattatgtga caatagatgt gagaatctga ttatactcgg aattggtgga gaaaacggaa
     1921 aaatcgtcaa attcgacctt taaggtcttg ccagatctgt atagattaac tgatgattcc
     1981 acgcaagatc ggcgcatgac ccgatcgaca aggtttcaac attgacaaag ggatttacat
     2041 catggctgtg aacaagaccg caggagtgac gaagatgaaa gttgggtcgg tttcgaaaaa
     2101 ggccaccgcc ctcaagaaag cggctcccac gaaggccaag ccttcaccag tttccaagaa
     2161 ggccacgccc gcgcttgcct ccaagaaagt tgctatcaag aaggccgcac cgaagaaggc
     2221 tgccggcccc aagctgacgg atccgcagca aaacatgctc aaaaaggtgg tcgcccacca
     2281 gggctccctc ggataccacc ctgccaaagt tgagaacaag gtcttggaga cgctgctcaa
     2341 acacaagttg gtcaagaagg gcaagaagca ccctgagacc aagctgtttc attaccaagt
     2401 ctcgaccatc ggcaagaagc acttggaaat gtcagccaag agctgaggca cggtgaatcg
     2461 atcgcatgtg gttcttgatg agagtgtcac cgtgatctcg acatcacggt gacactctca
     2521 tgcccgtgat cctctcacca agatgttctc gttaccatga tggtgtttcc tgcgaaacgc
     2581 tcctttcaac cttcgggtga aaggagcgtt ttgcgctttc tgtccgctat tgagcattgc
     2641 ggtcaagcca gttttttata ggacgtgaaa atgccggcgc gggccatcgt acgttcctca
     2701 atggtgcgca tcgtaggcgc gtcaaatggt tgaaactcgc gggcgattcg agcattctcg
     2761 tcaacctcgg ccgggctcga gcaaccgatg acgacggttg agacgccggc aaggctcaag
     2821 acataaccca tggcttcaga tgaggcgact ggaagcgatc ttgcgtcacc tcgaagcaaa
     2881 gttcccgcgg catagacttt cataccgatc acgcccatgc cgcgtcgcgc tgcctcaacc
     2941 aacgcggttt gagcgaaagg caagcgatgg acatcggccg cattgagcgc caccagcacg
     3001 gtatcgaaat cgaactggcg catcgcttcg agcaggatgg ccggatcatg atgcccagtc
     3061 acgccaagga accggactcg tccctcgtcg cgcgcctgga caagcgcttc gagtgcgccc
     3121 cgacggccaa agatcatgtt gaggtcgctc ggagttcgca gatcgtggag ttgccacaag
     3181 tcgaggtgat tggttcgcag ccgtttcagg ctttcatcga ggagccgaag cgagccgtcg
     3241 cgcgtacgat cgtgggtctt ggaggccagg aagacctggt tgcgccgctc gcccagagct
     3301 gcgccatagt aatcaatact gcctgcgtag gccggcgacg tgtcacagta cgtcacccct
     3361 tggtcgaggg cgcggtggat aaccgcgacg gcttccgcca tgcgtccatg agtacggagc
     3421 acgccttcgc ctcccaggcc aaattgggaa acctcacagc cggtcttgcc cagcaaacga
     3481 tgagggattg gcggataagt aggtgtgctg atgctggtat gggtcgacgt cgtgagcatt
     3541 ggaattactt cctccgggcc gctcaaacga attgcagttc gacgatcgag cagtcgtcga
     3601 cgaactcttc tcgaccttga atctcccgat cgtaggcgat caggcgatcc attttggagt
     3661 cgccggcgat gtcgtgagga ccctttttca tgaactcgat aaattcattg aacggccaca
     3721 tggacttgtc ggtccgctca atttcgtaga cgccgtcact gtagagaaaa agcttggcga
     3781 atgagccgag cgaggtagtt gcggtcttaa actcgagatc gggaaccgct ccgatcatcg
     3841 ggccgcccgc ttcgaggatc tcgagtgcag cttggtccgc ggattgcccg ttgaaaagga
     3901 gcacaggtgg atggcctccc ccggcgtagt cgattcgccg cgtgggcttg tgatagacac
     3961 cataccagat ggtgaagaag agaccgttct gttgatccat ctggaaggca ttgttgagcg
     4021 cgcccaagac ctgaccgggc gcgcgaaaat cagtctgagg tagcgcttgc gatcgcagcg
     4081 cgttcatagc cgagaccgag agcaacgccg atccgacacc atgaccgctt acatcgagca
     4141 ggtagaatgc gaaatgatcg tcatcgagcc aatggtaccc aaaggaatcg cctcctaact
     4201 cagcggaggg gacaaagcgc cagtcggtcc ggacgtctcc cttcctgagt ttgtcgggca
     4261 aaagagactg cacgtaatgg gcggcctgct tcatctcgtt ggccaacctt tgctgacttt
     4321 caaggagtgc cccgtaggcc tcgttacgct gaagcaggtt gatataaccc ttggagtgat
     4381 agcggatccg cgccaggagc tcgagccggt cgggaagctt gacgatgtag tcattggccc
     4441 ccaaggcgaa cgcctcggcc ttgacggttg gctcttcctt ggtcgagagc acgatcaagg
     4501 gggtctcctt ggtcggttcg tgagcccgga acgctttcac caaagttaga ccgtcgatgt
     4561 cgggcatgac gaggtcttgc aggatcacag taggcttgag cctatctgcc tcgtcaagcg
     4621 ctttgcgggc gtcgcggttg tagtgaaaaa caatgtcggg ttcgccggca agcatgcggc
     4681 ggacggcctc accgatcatg ggctgatcgt cgaccagcaa aaccgtgacc ttgtgctggg
     4741 tcaagacagg ttcgatgctc atgcacgaga ctcccggcgg ctcgagacta agtggatgcc
     4801 gatctcaaat cactaaaagc ataaattctt tgatgatctg cgaaacgggc tgcggtttga
     4861 ccatacgagg ccgcgggctc ggcgctactt gggaacgagc cgaacgatcg cgtcggcgac
     4921 gcgtgaaatg ggcaaaactt gctcggccgc accaatctcg gcggcggcct tgggcattcc
     4981 ccaaacgacg ctcgacgact cgtcctgggc gatcgtccgc caatcttgcc cgcgcagttt
     5041 gagcagcccc gctgctccat cgcgacccat gccggtcaac agagccgcta cacccggctt
     5101 gggccaattc cgggccacgc tggcaaaaaa cacatcgacc gaggggcgat aactcacgtc
     5161 gctgggctcg gcagaataat gaaggcaacg atcctcaccc agaatgagat gatcgtccgt
     5221 gcctgagagc agcacatcgc gggccgcggg acgatgcccc tctgtgatca acgtcacgtg
     5281 ccggtgcgac tgttccgaaa gccattgtcc cagtccaggt gcgaatgccg cgtcaacgtg
     5341 ctgcacgatg atgatcggaa cttccagggt ggccggtatc tcggcgagaa cctgagccaa
     5401 ggcgttggga ccgccagttg acgcccccaa aatcaccagg ggctcgatta actccgcact
     5461 gttcaaagga tgcagtgtgt gaatcaagga atcagccgcc tgccgtcgat cgccgtgccg
     5521 ttcggccgtg tttcccagca gcttgccgat aatctcgatc ttgtgcaaga gaagcgaagc
     5581 cccggagatc tggccgtgga ggcccaatat cggcgtgtcg agcgcatcga gggcgccata
     5641 gcccatcgct tggtagactt tactcagatg acccgagatc gtcgccgtca ccaccaatat
     5701 cgcgcacggc gattcaccca tgattcgccg cgtagcctcg accccgtctg tgcccggcat
     5761 gaatagatcc atcagaatca gatcgggccg gtcctcgcgc gccagtgcaa tcgcctcggc
     5821 accgtcgctg gcagtccagg caatttcgtg ttgggaacac gactggacca cccggcgcag
     5881 agcctcgcgc gccagaccaa tgtcgttgac gatcccgatt cgcatttgac gcgacttcga
     5941 ctcctgggag cccaccgctc aaagcagcaa ctgtaaatga gaaaccccaa ctcatgcaag
     6001 cggctcttgg tcgagccgtg ccttggttcc gtcgagtttc tattgtaaca cgaccataat
     6061 cactgccgcg gctcgccaat caagtcggcc actgttgtca aaaacgtccg gtcgtggaag
     6121 ctgcttttgg taaggtagaa gttggcgccg gcatcgagcc ctctgattcg gtcctcctcg
     6181 cggtctttgt acgagacgat caccaccgga atggccttga gccgggcgtc ttccttaatg
     6241 tgctggacaa gctggatgcc gtccattcgc ggcatatcaa tgtcgctcac gaccaggtca
     6301 taatgggtac tccggaccgc gttccagccg tcaacgccat cgacggcgac atcaacagtg
     6361 taaccgcggt tttccagcaa ctgcctttct agctcacgga ctgtgataga atcgtcgacg
     6421 acca
//
LOCUS       DATAQL010000227         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3381662, whole genome shotgun sequence.
ACCESSION   DATAQL010000227 DATAQL010000000
VERSION     DATAQL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3381662"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..620)
                     /locus_tag="VHS97_03895"
     CDS             complement(66..620)
                     /locus_tag="VHS97_03895"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX3447365.1"
                     /translation="MPVKIYTKTGDLGMTGLLGNRRVPKDDARIEAYGTADELNAMLG
                     VVRAHGLDTAADGLLADVQGDLFVVGSALADPAPGGPFHNVITDQHVARLESAIDTLE
                     TELKPLNQFILPGGTLAAAQIHLARTICRRAERLTVKLSRQPGEDVPSTLIVYLNRLS
                     DFLFVLARTVNHRADVPDIPWKGL"
     gene            837..1934
                     /locus_tag="VHS97_03900"
     CDS             837..1934
                     /locus_tag="VHS97_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447366.1"
                     /translation="MSSTLSTLFLAAALAAQHPGASPSAAPALSAEEVARLEAPHLKN
                     VRQVTSGLAKAGEGYFSADGRSIIFQAVPYAAPTIFHQPKPDEDGYQIYLGELNQDAP
                     AKMVSTGKGRCTCSFFHPDGKSILFGSTHLSPSTEAAPSKGPAYSRTERYRWEFPESM
                     DIFSADRDGGNLKRLTDTPGYDAEGSYSADGSRIVFTSFRDGDAEIYIMDADGKNPRR
                     ITHAKGYDGGPFFSPDGSRIIYRSDRLGNDLLQVYVNNTDGTAERALTKNEFVNWGPY
                     WHPDGRHIIYATSKHGHSNYELYLMNVDTGDEVRITYHEGFDGLPVFSPDAKKLMWTS
                     SGRTSDRKSQLFIADFTLEPAPRSAPRAAAR"
     gene            2018..2470
                     /locus_tag="VHS97_03905"
     CDS             2018..2470
                     /locus_tag="VHS97_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3447367.1"
                     /translation="MSDFIMDKYLADLSDEDIRLRPIEGMHPIALQLGHLLAGERMFI
                     EMVNPGSSPPLPEGFKDAHDLKNKELTDAGFLSKDKYVELMKAQRVATLATLEAVPDS
                     DLDDNRDGKLPAWLPTVGAALLFAGLHSINHTGQFVAVRRALNKPIAF"
     gene            complement(2474..3292)
                     /locus_tag="VHS97_03910"
     CDS             complement(2474..3292)
                     /locus_tag="VHS97_03910"
                     /inference="COORDINATES: protein motif:HMM:NF021365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX3447368.1"
                     /translation="MAWTLPDDCVLGILGERPEPGVLPQLAEGEHRSESLSSRTTIEA
                     RPRRSAEGHKDHLKNGLVIEWGVEAAAVLQQAMLFDTLDIWDSESVLAPGGRRVLLYA
                     PGDAGPWFDALLPASFALQPQVGADPGECLHAFFSGELEEGASRVVLIGSDSPTIDPT
                     IVVSAFLCLEGRDVVLGPATDGGVYLVGARGSVPPIFDGIDWNRPNALRQSVDRLTDT
                     GLSLSLLPPWYRINQPNDVRMLAGHIRALRRAGLDPGIPRVERLIEREWLARLA"
     gene            complement(3294..>3698)
                     /locus_tag="VHS97_03915"
     CDS             complement(3294..>3698)
                     /locus_tag="VHS97_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX3447369.1"
                     /translation="QRFLIIWWRCGRCRARPNANYLEEPMTSLLTRLLRRSPRAEHLT
                     LTVYTRAQCCCCHKALDVLKDAQQRSGFGIEEVDIDADPNLITLYDTEVPVVALNGKV
                     RFRGVVNPALLERLLVAESAKQSDDKLQLEGN"
BASE COUNT          762 a         1166 c         1085 g          685 t
ORIGIN      
        1 cggtgatcag cacaaccttg tcttgtaatg actgggccgt tgtccggtct gctatggtcg
       61 ccatttcaca gccccttcca gggaatatcg ggcacgtccg cgcggtgatt gacagtgcgg
      121 gcgaggacga acaggaagtc gctcagacga ttcaaataga cgatcaacgt actgggaacg
      181 tcctcacccg gctggcgcga tagcttgacg gtcaatctct cggcgcggcg gcaaatcgtt
      241 cgcgcaaggt ggatctgcgc cgccgcgagc gtgccgcccg gcaaaatgaa ctggttcaaa
      301 ggcttcaact cggtttcgag cgtgtcgatc gccgattcga ggcgtgcgac gtgctggtca
      361 gtgatgacgt tgtgaaacgg gccgccgggc gccggatccg cgagcgccga gccgacgaca
      421 aacaggtccc cctggacgtc cgcgagcaga ccgtcggccg ccgtgtccaa gccgtgcgct
      481 cgcacgacgc cgagcatggc gttcagctcg tcggcagtgc cataagcctc gatccgggca
      541 tcgtccttgg gcacacgacg atttcccaag agacccgtca tccctagatc gcccgtcttc
      601 gtatagattt tcactggcat tcctcgttcg acccagcccg tgtgtacccg ttcgcgttcg
      661 atgcgaggtg aaacctcgac cattggcggt cgctttgtcc ggaattatac tatgcgcatt
      721 gctcggcacc gagtaggggt gaaagagcgg tcgaaccgac aacctgcgcc gcgatttgac
      781 tattacttcc gctcgccgcc ctacgccaac accgaattcg agacggagtc cgctctatgt
      841 cttcaacgct atcaacccta ttcctggcgg cggctctggc cgcccagcac cctggggcca
      901 gcccttccgc agcgccggca ctctcggccg aggaggtggc tcgtctcgaa gccccccatc
      961 tgaagaacgt tcgccaggtg acgtccgggc ttgccaaggc gggcgaaggg tacttcagtg
     1021 ccgacggccg ctcgatcatc tttcaggcgg ttccctatgc ggctcctacg atctttcacc
     1081 agcccaagcc cgatgaggat gggtatcaaa tctatcttgg tgagctcaac caggatgctc
     1141 ccgccaaaat ggtcagtacg ggtaaaggac gctgcacctg ctcgttcttc caccccgatg
     1201 gcaaatcgat cctgttcggg tcgacccacc tgagccccag caccgaggcg gcgccctcca
     1261 agggtccggc atacagccgg accgagcggt atcgctggga gtttcccgag tctatggaca
     1321 tcttcagcgc ggatcgggac ggggggaact tgaaacgact gaccgacaca cccggctacg
     1381 acgccgaggg aagctattcg gccgacggct cgcggatcgt ctttacttcg ttccgtgacg
     1441 gcgacgccga gatctacatc atggacgccg atgggaaaaa cccgcgccgg atcacgcacg
     1501 ccaagggtta cgacggcggc ccgtttttct ctcctgatgg ctcgcggatc atctatcgca
     1561 gcgaccgcct ggggaacgac ctcttgcagg tttatgtcaa caataccgac gggaccgcgg
     1621 agcgcgcact gacgaagaac gagttcgtca actgggggcc ctattggcat cccgacggcc
     1681 gccacatcat ctatgcgaca agcaagcatg gccattccaa ttatgaactg tatttgatga
     1741 atgtggacac cggtgacgaa gtgcggatca cctaccacga aggcttcgac ggtttgcccg
     1801 tgtttagtcc ggacgccaag aagctcatgt ggacctcgag cggccgcaca tccgaccgca
     1861 agagccagct tttcatcgcg gactttaccc tggagccagc gccacgctca gcccccaggg
     1921 cggcggcgcg ctaagtgtga cctttcaaca acattaacgc caacaggaga tcaagaccgc
     1981 tatgggacca aaggacgtta tcaagcagac tctagacatg agcgacttca ttatggacaa
     2041 atacctggca gatctgtcgg acgaagacat ccggctgcga ccgatcgaag gcatgcaccc
     2101 gatcgccctg cagctcggtc acctgctcgc cggcgagcgg atgttcattg aaatggttaa
     2161 tcccggttcg tcacctcctt tgcccgaagg gttcaaggac gcgcatgacc tgaagaacaa
     2221 agaactgacc gacgcgggtt ttctctccaa ggacaaatac gttgagctca tgaaggccca
     2281 gcgagttgcg actctggcga cgctcgaggc cgtgcccgac tcggatctgg atgacaatcg
     2341 tgatggcaaa ctccctgcct ggctgccgac cgttggagcc gcgttgctct tcgccggcct
     2401 gcattcgata aaccacaccg gccagtttgt agcggtgcgg cgggcgctca ataagccgat
     2461 tgccttctaa ccgtcaggcg agccgcgcca gccattccct ttcgatcaat cgttcgacac
     2521 gagggatgcc cgggtccagg cccgcgcgac gcagcgcgcg aatgtgcccg gcgagcatcc
     2581 gcacgtcgtt gggctgattg atccggtacc agggaggcag aagcgacagt gacagcccgg
     2641 tatccgtgag ccggtcgacc gattgcctca gggcatttgg gcgattccaa tcgataccgt
     2701 cgaagatcgg cgggactgaa ccgcgagctc cgaccaagta gactccgccg tcggtcgccg
     2761 gcccgagaac gacgtcgcgg ccttccagac agagaaacgc actgacgacg atcgtcggat
     2821 cgatcgtcgg cgagtcggaa ccgatcagga ccacgcgcga cgcaccttcc tcgagctcgc
     2881 cactaaaaaa cgcgtgaagg cattcacccg gatcggcgcc gacttgcggc tgcaaggcga
     2941 atgaagcggg caagagcgcg tcgaaccagg ggccggcgtc gccgggagcg tacagaagga
     3001 cgcgccggcc gcccggagcg agtaccgact cggaatccca aatgtcgagc gtatcgaaga
     3061 gcatcgcctg ttgaagcacg gcggccgcct caactcccca ttcgatgaca agcccgttct
     3121 tgagatggtc tttatggcct tctgcggatc gacgaggccg agcctcgatc gtagtccggc
     3181 tcgaaagtga ttccgagcga tgctccccct ccgccaactg cggcagaacg cctggctcgg
     3241 gtctctcgcc caagattccg agcacacagt catcgggaag ggtccaggcc acgctaattc
     3301 ccctcaagct gcagcttgtc gtccgactgc ttggcgctct cggcaacgag caatctctcc
     3361 agaagcgcgg gattgaccac gcctcggaac cgcaccttgc cattgagcgc cacgactgga
     3421 acctcagtgt cgtacaacgt gatgaggttg ggatcagcgt cgatatcgac ttcctcgatc
     3481 ccgaaaccgg atcgctgctg cgcgtccttg agcacatcga gcgccttatg gcagcaacaa
     3541 cactgggcgc gggtgtaaac ggtgagtgtc aagtgctctg cccgcggcga ccggcgcaga
     3601 agtcgtgtca gcaatgaagt catcggttcc tcgaggtaat tagcgttcgg gcgagctcga
     3661 caccgcccgc acctccacca gattatcaaa aaacgttg
//
LOCUS       DATAQL010000228         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4145755, whole genome shotgun sequence.
ACCESSION   DATAQL010000228 DATAQL010000000
VERSION     DATAQL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4145755"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(522..881)
                     /locus_tag="VHS97_03920"
     CDS             complement(522..881)
                     /locus_tag="VHS97_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447370.1"
                     /translation="MSNLSRTKADNLDLAGWFPVNAEKHWWAANGVARMAKHYATTES
                     RIDEVKIGFELLLPSGIDGVLPTTFVIDGIKLIHNNGAGEPPKIRLTSDLPEGPYSVD
                     FPLDAVVTMIAEPTDDD"
     gene            1188..1373
                     /locus_tag="VHS97_03925"
     CDS             1188..1373
                     /locus_tag="VHS97_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447371.1"
                     /translation="MNTNPNQRHSYGDWRDMTVEELRDRLHLQVTSWWRHVTLMDLYE
                     DPQTKGGLLQIARSEGE"
     gene            1375..1572
                     /locus_tag="VHS97_03930"
     CDS             1375..1572
                     /locus_tag="VHS97_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447372.1"
                     /translation="MTEAADRAREANAHFLRARVSIVDPTVMELIAGCAVLATAIEAL
                     AVELHDMKQKSGFIREKSHLR"
BASE COUNT          329 a          512 c          515 g          299 t
ORIGIN      
        1 cctgatggtc gcccttcata ccgtccgggt gcaacgcgac gaagccgcgg ccgaggcagc
       61 gcggctgcgt cggcgagtcg acgaattgag cgcccaggca ccctcgcacc tactgccaac
      121 cgacccaatt cccaaccggc agcacgccag ttaaacgcgg gcggcgatca ggtgaagtgc
      181 taaaccccaa cgcggggccc tacgctcccg cgcatgcgcg acatcgaaac catcgactcc
      241 gaggcggtga gagcgtcggc cgcacctcgt ttagccaggc catcgccagc atcttcggca
      301 accacgacgt cggtgacgtg caagcagcag atgggatttc accgagcact gccgacggct
      361 catcgagtcc cagaccggag tgaagtgcca gcgcgcttgt cccggattta ggctccgcgc
      421 gtgcgcgaca tcgcggccat cgactccgag ctgcgattgg tggcggcact tcgccgtgcg
      481 gctcgggagc ggggtgggcc gctgccgtcg atagacgtgg ctcagtcgtc gtccgtgggc
      541 tcggcgatca tggtgacaac ggcgtcaagc gggaagtcga cgctgtacgg cccttcaggg
      601 agatcagacg tcagcctgat cttcggtggt tcgccagcgc cgttattgtg gatgagcttg
      661 atcccgtcga tgacgaaggt cgtaggcagt acaccatcta tgccggacgg caagagcagt
      721 tcgaaaccga tcttcacttc gtcgatgcgg ctttcggtgg tcgcgtagtg tttcgccatc
      781 cgcgcaaccc cattggccgc ccaccagtgt ttttcggcgt tgaccggaaa ccaccccgcc
      841 agatcgaggt tgtcggcttt cgtacggctc aggttgctca tttagctctc ctcgggggcg
      901 gggcccggtc ctcatcgggt gctgtctacg cgagcagcca atcccagcgc gggagttgat
      961 ggctacccag gctgacgctt agcaagccgg caggcggctt gtttggccgc cgactcgagt
     1021 acgatcatac cgataatcgc ttgaatcggc ttcttagata cttaaaagat tgtgtaatgg
     1081 aaataccaga cttttagtcc ggcgcatcgg cgcgtgctac gtcgacatgc tccgggcacc
     1141 gcgcggtgtc tggcacgcct aacccgaatg aagggctgtg accaccgatg aacacaaacc
     1201 ccaaccaacg ccacagctat ggggactggc gcgacatgac cgtagaagaa ctgcgcgacc
     1261 ggctgcacct ccaagtcacg tcgtggtggc gccacgtgac actgatggac ctatacgaag
     1321 acccgcaaac gaagggcggg ctcttacaga tagctcgatc cgagggggaa tgaaatgacc
     1381 gaggccgccg acagggccag agaggcgaac gcccacttct tgcgggcgcg agtttcgatc
     1441 gtcgacccca ccgtaatgga gctgattgcc ggttgtgctg tactcgccac agcgatagag
     1501 gccctggcgg tcgaactgca cgacatgaag caaaagtctg ggttcatccg cgaaaaaagt
     1561 caccttcggt gaggggtggc tgggtcacag ccacgagacg ttgccgccac ccggcctgta
     1621 gccggggcca cattacgttc cgcgagaccg tgtaa
//
LOCUS       DATAQL010000229         6917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_482844, whole genome shotgun sequence.
ACCESSION   DATAQL010000229 DATAQL010000000
VERSION     DATAQL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482844"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..3587)
                     /locus_tag="VHS97_03935"
     CDS             complement(12..3587)
                     /locus_tag="VHS97_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX3447373.1"
                     /translation="MMGIAQKVVRHFSDAVRSRGQSYFVKGRVTVMAARPGEVVAKVR
                     GTSKYRVRLRMRGSKLLASCTCPYFSPQGEPCKHLWATLLLAEARGMLQSPPVYPVHL
                     VTESPRRSTTVGLPETRNESGIEREPSRPNQTDLGIGAVDLGLSRPSGPPPRGPRPKG
                     KDRGIRRDQPGEAGSWSQRGHAPRDYRAGSTGRSKTKLPGPGHGTGSGPARAKAVNRN
                     SKRLLVYVLDVAATLAQNQVVIDLARRQRKTTGEWGPLRPWYYAPHASHVKYDPEDRL
                     ILALLDEAQGAAPHAGYLPPGLNAGSVSGSGPTNGVATAVGMRQPGAYGSAAAARAAS
                     ERALRGMRRFVLRKDQAALVERLARTGRLRLRRTEGEEDPPTVRWDDAQPWRFSLEIR
                     PELGGKRWAWRGSLRRGENRMDLSEPLVLLPGLLILGVGRAARFDDLGVMPWIQRLRY
                     EKEITFVEPQQDLMLGRILAETRVPPLELVESLRLEEINAKPRPSLTLRTPRQNWGLG
                     SDKLIGELEFDYDGAVIPAGRTTPLAVSTELGLVIRRDPRTESAADVKLFELGFREAK
                     DPRLDPGTLELPAKRMAQVSKELVIAGWRVEAEGKLIRPAGEFKMALATGIDWFELDG
                     GIQYGDQKVNLPDLLAAARRGESMIELGDGSMGMLPEEWLKKYGMLADLATTADGSLR
                     FGSSQAGMLDALLAAQPEIEVDAAFEKVRQNLRQFEGVVAVDSPPGFHGELRPYQREG
                     LGWLDYLQRFGFGGILADDMGLGKTIQVLALLQRRRAHRQAKGPSLAVVPRSLVFNWI
                     QEAAKFTPRLRILDYTGPGRHALREAFADYDLIITTYGTVRTDIAELTNFQFDYAILD
                     EAQAIKNAESQSAKAARLLRSRFKLAISGTPIENHLGELWSIFEFLNPGMLGSDSVFK
                     KYVGSGASLEQTDRTLLAKALRPFILRRTKQQVVEDLPEKTEQTLYCDMEAEQRRCYD
                     ELRAHYRDALLRKDTAELNRSKIEVLEALLRLRQAACHPGLISPELASEPSTKLEMLI
                     PSISEVVAEGHKVLVFSQFTSFLAIVRERLEQENIVYEYLDGRTRNRAAKVERFQNDP
                     DCPVFLISLKAGGLGLNLTSAEYVYLLDPWWNPAVEAQAIDRSHRIGQTQHVFAYRLI
                     CRGTVEEKILELQQKKRDLADAILSADNRGVIQNLSRDDLEFLLS"
     gene            complement(3964..6516)
                     /gene="gyrB"
                     /locus_tag="VHS97_03940"
     CDS             complement(3964..6516)
                     /gene="gyrB"
                     /locus_tag="VHS97_03940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX3447374.1"
                     /translation="MADQVAISDYTGASIRVLEGIEAVRLRPGMYIGDTTTRGLHHLV
                     YEVVDNSIDEAMAGYCRNIHVTLHTDGSLSVIDDGRGIPVDIHADSGLSALEVVLTKV
                     HAGGKFDHDTYKVSGGLHGVGVTVVNALSEWLEAEVRRDGQIWRQEYRKGQPEGSVTP
                     KGPTKTTGTTIRFLPDGEIFPKIDFDFDVLEKRLRELAFLNKGIRIRLTDERTDEPRD
                     VAFFSSEGLSEFVVFLNRAQTVLHQPIVFSGRDEERAVEVEIALQYNDSISEMVVSYC
                     NNINTIEGGTHLTGFRTALTRTLNQYAKTASSAKTKDLTILGEDFKEGLTAIISVRVP
                     DPQFEGQTKTKLGNSDVEGITARVVNERLAEFLETNPGVGKRIIVKAQLAAEAREAAR
                     KARELVRNRKGVLSGGGLPGKLMDCSTHDQDSSELFLVEGDSAGGTAEGGRDRIYQAI
                     LPLRGKILNVERARLDRVLSNEEIRNIITAVGNSIGEEEDPAKRRYGKIVMMSDADVD
                     GSHIRTLLMTFFYRQMPKLVAEGHLYVAQPPLYLIVSGKKERRYVHAEDQMQTILMET
                     GLAGALLHTAAPETTERANEISGDRLKGLLDLVASIEQGLRAFGRRNRSVRDFLAMAY
                     KPKADDSAKPDPRDGLLPLFLVEHEGKEERFYSEKERQDYLAAHNLQLDVETELEPSS
                     GLTAVPDATGTGAIASQNASPDHRRVREVELHEVKMLNKHLIRLRDEFDLRADVLLPR
                     EVTGDDPPPRFVLERDGESHPLLDLRALVSTVRKIGEKGMKITRFKGLGEMDAEQLWE
                     TTMDPARRTLMQVRLDDVAAANDLFGTLMGDDVEPRREFIEKHALEVKNLDV"
BASE COUNT         1385 a         2091 c         1915 g         1526 t
ORIGIN      
        1 ggttttgccg ctcacgatag gagaaactcg aggtcatcgc gggacaggtt ctgaatgaca
       61 ccccgattgt cggcgctcaa gatcgcgtcg gccagatccc gcttcttctg ctgcaattcc
      121 agaatttttt cctcgacggt cccccggcag atcagcctgt aggcgaatac atgctgagtt
      181 tggccgatcc ggtgggaacg gtcgatggcc tgagcttcga cggccgggtt ccaccaggga
      241 tcgaggagat aaacatattc agcggaagtc aggttcagtc ccaagccgcc ggccttaagg
      301 ctgatcagaa ataccggaca gtcgggatcg ttctgaaacc gttccacctt ggcagcacga
      361 ttgcgggtcc gtccatccag atattcatag acgatattct cctgctccag ccgttcgcgg
      421 acaatcgcca ggaagctagt aaactgagaa aagaccaaga ccttgtgccc ttcggcaacg
      481 acttcggaaa tcgatgggat gagcatttct agcttggtgc tgggctcgga ggctagttcg
      541 gggctgatta aacctggatg acaagcagct tggcgaagcc gcaacagggc ttcgagcact
      601 tcgattttgg accggttcaa ttcggctgta tcttttcgca agagcgcatc gcggtaatga
      661 gcccgcagct cgtcatagca gcgcctttgc tcagcttcca tatcgcaata caacgtttgc
      721 tcggtctttt cgggtagatc ttcgacgacc tgctgcttgg tacgacgtag gatgaacggc
      781 cggagagcct tggctagcaa cgtgcggtcg gtttgttcca gggacgcgcc gctgccaaca
      841 tacttcttga agaccgaatc gctgccgagc attccggggt tcaaaaactc gaagatcgac
      901 cagagctcac ccagatggtt ttcgatagga gtgccgctta tggccagctt gaatcggctg
      961 cgcaacagcc gtgctgcctt tgctgactgg ctctcagcat tcttgattgc ctgggcttca
     1021 tccaatatcg cgtagtcaaa ctggaagttg gtcagttcgg caatgtccgt gcgcaccgtg
     1081 ccgtacgttg tgatgatcaa gtcgtagtcg gcaaatgcct cacgcagcgc atgccggccg
     1141 gggcctgtat agtcgagtat gcgaaggcgc ggggtaaact tggcggcctc ttgtatccag
     1201 ttaaagacca gcgagcgcgg gacgacagcc agagacggtc ctttggcctg acggtgagcc
     1261 cgtcgccgtt gcaaaagtgc cagtacctga atcgtcttac ccagacccat gtcatcggcc
     1321 agaatgccac caaagccaaa gcgttgaagg tagtcaagcc agcctaatcc ctcgcgttga
     1381 taagggcgaa gctcgccatg aaagcctggg ggcgaatcga cagctactac cccctcgaac
     1441 tgccgcaggt tctggcggac cttttcaaaa gccgcatcaa cctcgatttc cggctgagca
     1501 gccagaagcg catccagcat gcccgcctgt gacgaaccga agcggaggct accatcagcc
     1561 gtggtggcca gatcggcgag cataccgtac ttcttgagcc actcctcggg aagcattccc
     1621 atcgagccat ctcccagctc gatcatggac tcaccgcgcc gggctgctgc caaaagatca
     1681 gggaggttga ccttctggtc accgtattgg atcccgccgt cgagttcaaa ccaatcgatg
     1741 cccgtggcca gcgccatttt gaactcgccc gctggacgga ttagttttcc ctctgcctct
     1801 acccgccatc cggctatgac cagttccttg cttacctgag ccattcgctt ggctgggagc
     1861 tcgagagtac ccggatcgag ccgtggatct ttggcttcgc ggaaacccag ctcgaaaagc
     1921 ttgacgtcgg cagcgctttc agtccgtgga tcacgacgaa tgacgagccc caactcggtc
     1981 gagacagcca acggagtagt ccggccagct ggtatcacag cgccgtcgta gtcgaactcc
     2041 agctccccaa tcagcttgtc ggaccctaat ccccagttct gtcgcggagt tcgtagtgtc
     2101 agtgaaggac gtggcttggc gttaatttct tccagccgca gcgactccac aagctctaga
     2161 gggggaacac gagtctcggc caaaatccgc cctagcatta agtcctgttg tggttcgacg
     2221 aatgtgattt ctttctcgta acggagccgt tggatccagg gcatgactcc taaatcgtcg
     2281 aaccgggcag cgcgaccgac gcctaggatc aacaagcccg gtaaaaggac cagcggttcg
     2341 gaaaggtcca tccggttttc accccggcgg agcgacccgc gccaagccca gcgctttccc
     2401 cctaactcag ggcggatttc cagcgagaac cgccacggtt gggcatcgtc ccagcgcact
     2461 gttggcggat cttcctctcc ctctgtgcgg cgcagccgta gccgtcctgt tcgcgccagt
     2521 cgctcaacca aggccgcctg atctttccgc aacacaaatc gccgcatccc gcgtaacgcg
     2581 cgctctgagg cggctcgagc tgctgcagcg gaaccataag cccctggctg gcgcatcccg
     2641 acggcggtag ccacaccgtt ggtcggccca gagccagaca ccgagcccgc atttaagcct
     2701 ggcggtaaat accctgcatg aggtgcggcc ccttgggctt catcgagcag ggccagaatc
     2761 agacgatctt caggatcata cttaacatgg gaggcgtgcg gagcgtaata ccaggggcgt
     2821 agtggtcccc attcgccggt tgtcttgcgc tggcggcggg ccaagtcgat gaccacctgg
     2881 ttttgcgcga gcgtggctgc gacgtcaaga acatacacga gcagccgctt ggaattccga
     2941 ttgacggcct tggctcttgc cggcccagag cctgtgccat gtccgggtcc cggaagcttg
     3001 gtctttgagc ggcctgtgct accggcacgg tagtcgcggg gagcatgacc ccgctgactc
     3061 caactaccgg cctcacccgg ttgatctcga cggatgcccc tgtctttacc cttgggcctg
     3121 ggtccccgcg ggggtggtcc agacggccgt gataggccca ggtcgactgc tcctatcccc
     3181 aaatccgtct gattcgggcg cgacggctcc cgctcgatgc ctgactcgtt cctcgtttcg
     3241 ggtaaaccta ccgtcgtcga gcgccgaggg ctctcagtca ccaggtgaac aggatacacc
     3301 ggtggcgatt gcaacatgcc ccgtgcctcg gccaagagca atgtcgccca aaggtgctta
     3361 cacggctccc cctgcggact gaaatacggg caagtacatg aggccagtaa tttggaccct
     3421 cgcatgcgca accgtacccg gtatttcgag gtccctcgta ctttggcgac aacctcaccg
     3481 ggtctggccg ccatgacggt aacccgaccc ttgacaaaat acgactggcc tcgcgaccgg
     3541 accgcatcgc tgaaatgccg cacgactttt tgggcaatcc ccatcatctg ttcccgctcg
     3601 ggacggaccc tgcaatcaga ctggctagcg acatatggct agtaattggg tgacgatgcg
     3661 ccaatgaaca cgataaatgt tcaatgccgg aatcccgaat tccggattcg gaaggacttt
     3721 tgggatcagg caacctgatc aactcctcga tttcacattc ggcacaacac ggccttgaaa
     3781 cggtttgaat tctccccggg agggtgagat aaccaccgag ccgattcaat caacagggat
     3841 tgacctcctt gttgagatcg cctctctccg caggactgtt ttaggcaacc ggtaacgctt
     3901 ttttctcacc gatcactaat cacgatccac tcgtcactcg gaactctcca accgcctggt
     3961 gccttagacg tcgaggttct tgacctcgag tgcgtgtttt tcgatgaact cccggcgcgg
     4021 ctcgacatcg tctcccatca aggtgccaaa tagatcgttg gcggcggcga catcgtcgag
     4081 ccgcacttgc atcagcgtgc ggcgcgccgg gtccatggta gtctcccaca actgctcagc
     4141 gtccatctcg cccaagcctt tgaagcgggt aatcttcatc cctttttcgc caattttgcg
     4201 caccgtggac accagtgcgc gcaggtccaa gaggggatgg gactcaccgt ctctttcgag
     4261 cacaaaccgc ggcggaggat catcaccggt cacctcgcgg ggcagtaaaa catcggcccg
     4321 taggtcaaac tcgtcgcgga ggcggatcaa gtgcttgttc aacatcttga cctcgtgcaa
     4381 ctcgacctcg cggacgcgac gatgatcagg ggaagcgttc tgggaagcta tcgcccctgt
     4441 accagtggcg tcgggcacag cggttagtcc tgaactgggt tccaattccg tttccacatc
     4501 cagttgcaag ttatgcgccg ctaagtaatc ttgccgctcc ttttcgctat aaaagcgctc
     4561 ttctttaccc tcgtgctcaa ctaggaacaa agggagcaag ccatcgcgcg ggtctggttt
     4621 ggcggagtcg tcggccttgg gcttgtaggc cattgccaga aagtcgcgga ctgagcggtt
     4681 gcggcgaccg aaggcacgta gcccttgttc gatggaggca accaagtcga gcaggccctt
     4741 gaggcgatcg ccgctgatct cattggctcg ctcggtggtt tccggcgcgg cggtgtgtag
     4801 taaggcgccg gccaatccgg tctccatcag aatcgtctgc atctggtcct cggcatggac
     4861 gtatcgccgt tctttcttgc cactgacgat tagatacaga gggggctggg caacgtagag
     4921 gtggccctcg gccacgagtt ttggcatctg gcggtagaaa aaggtcatca agagggtccg
     4981 gatatgagaa ccatcgacgt cagcgtcgct catcatcacg atcttgccgt accggcgttt
     5041 ggcgggatct tcttcctcgc cgatgctgtt gccgacggcc gtgatgatat tgcggatttc
     5101 ttcattgctc aagacgcgat caagccgggc tcgctcgacg ttaagtatct taccgcgcaa
     5161 cggaagaatc gcctgataga tccggtctcg ccccccctct gctgtaccgc cggccgaatc
     5221 tccctcaacc agaaagagct cacttgaatc ttggtcgtgc gtggaacagt ccatcagctt
     5281 gcccggtaat cctccacccg agaggactcc cttgcggtta cgcactaact cacgtgcctt
     5341 gcgggcagcc tcgcgtgctt cagccgcgag ttgtgccttg acgatgatcc gcttgccgac
     5401 ccctgggttt gtctcgagaa actcggcgag ccgttcgttg acgactcgcg ctgtaattcc
     5461 ctcgacatcg ctgttgccca gttttgtctt ggtctggccc tcgaactgag ggtcgggcac
     5521 gcgcacgctg atgattgccg tgagcccttc tttgaaatcc tcgccgagga ttgtcaggtc
     5581 tttggttttg gccgagctgg cagttttggc gtactggttt agcgtgcgag ttaaggccgt
     5641 gcggaacccc gtaaggtgcg tgcccccttc gatggtattt atattgttac agtacgacac
     5701 aaccatctcg ctgatcgaat cgttgtactg taaagcgatc tcgacttcaa cggcgcgctc
     5761 ctcatctcgc cccgagaaga caatcggttg gtgcaaaacg gtttgagcgc gattcaaaaa
     5821 aacaacaaat tcgctcaagc cctctgatga gaaaaacgcc acatccctgg gctcgtccgt
     5881 acgctcatcg gtcagccgga ttcggattcc cttattgagg aaggccagct cgcggagcct
     5941 tttttcaaga acatcaaagt caaagtcgat cttggggaaa atctccccgt caggcagaaa
     6001 gcgaatcgtg gtgccggtcg ttttggtcgg gcccttgggc gtgactgacc cctcaggctg
     6061 ccctttgcga tactcttgcc tccagatctg gccatcgcgg cggacttcag cctccagcca
     6121 ctccgacagc gcgttgacaa ccgtcactcc taccccgtgc aaccccccag agaccttgta
     6181 ggtatcgtga tcgaacttgc cgccggcatg gaccttcgtc aagaccactt cgagcgcgct
     6241 taagccgctg tcggcatgaa tatcgaccgg gatgccccgt ccgtcgtcta tgaccgaaag
     6301 cgaaccatcg gtgtgcaacg ttacgtggat attccggcag tagcccgcca tcgcttcgtc
     6361 gatcgaattg tcaacaactt catagaccaa atgatgtagg ccgcgcgttg tcgtgtcgcc
     6421 aatatacatc ccggggcgca gccggaccgc ttcgattccc tctagtactc ggatactcgc
     6481 cccagtgtaa tccgagatgg ccacctgatc ggccatagcc acgtccgtat ggttcccctg
     6541 accattgccc gtctgttctt ttacctcgag attcgaatgt cccgggattg aagtctcaac
     6601 cgggcgatcc tcagttttgg tcaaatccgc cattacaggt ttacctcaat cttcgtctcg
     6661 tctcgtctcg tgctgaactt ccggccggag gccgtgggat agggcaggtg agacggtcat
     6721 gcctgccaga gatcatcacg aaggccggtt cccccgaccg gacatgactt gcatccaaag
     6781 gtatctcacc ctagaaggac agccgtggct ttagacacac tggtcctgcg tattcgcgac
     6841 catatctgcc atgaccgacg gtttgtgctt gtgggcgttt ggctgggctc cctaccactg
     6901 cgccatttga ccgacgg
//
LOCUS       DATAQL010000230         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2772272, whole genome shotgun sequence.
ACCESSION   DATAQL010000230 DATAQL010000000
VERSION     DATAQL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772272"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..800
                     /locus_tag="VHS97_03945"
     CDS             240..800
                     /locus_tag="VHS97_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447375.1"
                     /translation="MNGVFATLYTVFSLKTLGLSAATLGLVISVGGIGALSGALLAAR
                     FSGRLGLGRALILCMAGSRLAGLLIPMARGPAWLAVSCLVGHQLVGDALLMGYYVLAV
                     SLRQTVLPHDTLGRANATFHVSAGLLLPIGALLAGSIAAATDVRTALWISALGGLANP
                     VILQLSALRHLKAMPVAGHLDRSEVG"
     gene            complement(810..2195)
                     /locus_tag="VHS97_03950"
     CDS             complement(810..2195)
                     /locus_tag="VHS97_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447376.1"
                     /translation="MLRIPGSGALDCDGVTRRSFVQAGILGLGGLGWADLLRMRSRAS
                     AVPAAGLVRECAGRSVILFWLSGGPGHMETWDPKPDAPSQFRGPFGAIPTSLPGVHFG
                     ELLREEARLMDKLAIVRTVSHGSGDHTKGNHWMLTGFEGPAFNAPDNEVQRRPSLGSA
                     IARLKGERAGRTGLPPYIAVPNLRGGTDNLFHYAAYLGGYANPFVVESDPNDPSFRVK
                     NLRLPGGVSLGRLEDRRRMLAAMTELKRDAEPAFRDLDAYYHRAFDMLAGNEVARAFD
                     IAAEPPLVRDRYGRHTFGQSALLARRLVESGVPFVTVNCVPWDHHGTPPQLKTEEGAR
                     KLIPALDRAIAALVEDLIARGLYDSTLVVAMGEFGRTPRMNRDAGRDHWGKTFSVLFG
                     CGSMQMGQVIGRSSPRGEYVVDRPISPQDVAATVYHHLGIDGRSITFDDRTGRPTYLI
                     EHGEPILELVG"
     gene            complement(2421..3584)
                     /locus_tag="VHS97_03955"
     CDS             complement(2421..3584)
                     /locus_tag="VHS97_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX3447377.1"
                     /translation="MTFRAVGDQLAVVLDGMKLEGKIRDGKMELEQREKDETPMKMTG
                     TVERGTMSGDFTMGTMTGRWKATRIPVRPTDAPREHVFEPKEFHRVFSGAIPPVLHIY
                     PGDSVKTWSVDAGGVDAQGVRRSLGGNPQTGPFYIEGSLPGDTLVVKLKRIRLNRDTA
                     FSTGSLAASAITPGYVRNRKPVENFDSNWVLDREKGIARLAKPTEALKNYTVPLRPIL
                     GCVGVAPPGHMVFRSGYPGSFGGNMDYNRLREGVTLYLPVSQQGALLFMGDGHAAQGD
                     GELTGNALETSMDIEFSVDVIAGKSLAMPRAEDDEYLMAMGIAGSLTEAFQIATTMMA
                     RWLEDSYKLNAAEVASVLGTAMRYDIAEVVDPYVNVVAKVPKKALAQIPKADR"
BASE COUNT          632 a         1271 c         1136 g          736 t
ORIGIN      
        1 cggcctgggc ggcctgctca tccaggcgat cacggcgcca ctggcgatcg cgcttgacgc
       61 gatctccttc ttgatctctg cggtgctgct cggcgcgatc cgtcgcccgg aagtgcatgc
      121 agcgtccgac accggcggtc acgggccgac cttgatccac gacctacgcg tcgggctgcg
      181 ggcctgtgtg ctgcaccctt ggtacggccg gtatcctggt ctaggccgta tcggcgctgc
      241 tgaacggagt gttcgcgacg ttgtatacag ttttttcgct caagacgctt ggcctgagtg
      301 cagcgacgct gggcttggtg atcagtgtgg ggggaatcgg cgccctctcg ggcgcgctgt
      361 tggcggctcg gttctccggt cggctcggac tgggtcgggc cctcatcctc tgcatggccg
      421 gcagtcgctt ggctgggctc ttgatcccga tggcccgcgg ccccgcttgg ttggcggtct
      481 catgcttggt gggccaccag ctcgtggggg acgccctgct catgggctat tacgtccttg
      541 cggtcagcct gcgccagacc gtcttgcccc acgacacgct gggtcgcgcc aacgcgacgt
      601 ttcatgtctc ggccggattg ctgctaccca tcggcgcctt gctcgccggc tcgatcgccg
      661 cggcaaccga cgtccggacg gccctctgga tcagtgccct gggtggactg gccaatcctg
      721 tgatcctcca gctctccgca cttcgtcacc tgaaggcgat gcccgttgcc ggccatctgg
      781 atcgatccga ggtcgggtga gaaacacccc taccccacca gctccaggat cggctcgccg
      841 tgctcgatca ggtaggtcgg gcgcccggtt cgatcgtcga acgtgatcga gcgcccgtcg
      901 atgcccaggt ggtgatagac cgtcgcggcg acgtcttgcg ggctgatcgg ccggtcaacg
      961 acatattcgc cacgcggact gctccggccg atcacctggc ccatctgcat cgagccgcac
     1021 ccgaagagga cgctgaaggt cttgccccag tggtcgcggc cggcgtcacg gttcatccgc
     1081 ggagttcggc cgaactcacc catcgccaca accagcgtcg aatcgtacag accccgagcg
     1141 atcaggtcct caacgagggc ggcgattgca cgatcgagcg ccggaatcag cttgcgggcg
     1201 ccctcctcgg tcttgagctg gggaggcgta ccgtggtggt cccacggaac gcaattgaca
     1261 gtcacgaacg ggacgcccga ttcgaccagc cggcgcgcca agagggcact ctggccgaag
     1321 gtgtgccggc cgtagcggtc gcgaaccaag gggggctcgg cggcgatgtc gaaggcgcgc
     1381 gcgacctcat tgccggcgag catgtcgaag gcacgatgat aataggcgtc gaggtcgcgg
     1441 aacgccggct cggcgtcgcg cttgagctcg gtcatcgccg ccagcatgcg gcgccggtct
     1501 tcgaggcggc cgagcgagac gccgccggga agcctcagat tcttgactcg gaaggagggg
     1561 tcgttcgggt cggactcgac cacgaacggg ttggcgtagc cgccgagata ggccgcataa
     1621 tggaacaggt tgtccgttcc accgcgcagg tttggcacgg cgatgtatgg gggcaggccc
     1681 gttcggcccg cgcgctcacc cttaagccgc gcgatcgccg accccagcga cggccggcgc
     1741 tggacctcat tatccggggc gttgaaggcg ggtccctcga agccggtcag catccagtgg
     1801 ttgcccttgg tgtggtcgcc cgagccgtgg ctgacggtgc gaacgatcgc caatttgtcc
     1861 ataaggcgcg cctcttcacg caagagctca ccgaaatgaa cccccggcag gctggtggga
     1921 atcgcgccga atgggccgcg gaactggctg ggtgcgtcgg gtttgggatc ccacgtctcc
     1981 atgtggcccg gcccgccgct caaccagaaa aggatgacgc tcctgccggc gcattcgcga
     2041 accaggccag ccgcgggcac agccgaggcc ctcgagcgca ttcgcagcag gtcggcccag
     2101 ccgagaccgc caagcccgag gatgcccgcc tggacaaagc tgcgcctggt gacgccgtcg
     2161 cagtcaaggg caccgcttcc tgggatcctg agcatcagat cgttcctcgt gtctattcgc
     2221 cgcggttgga gcagggattt gctcgcttca ccgctcctgg tagcttcgga gatcgaccaa
     2281 accttcgcgc cgattcaaag agtttacact cggaccgcgc cggtcgcaaa tcagaccact
     2341 gatccaccac gacaccggtc cgatgctgac ccacaccgct cgacaggcag agccgttgat
     2401 tgtcaaaaca ttcgatggct ttaccggtcc gccttcggaa tttgcgccag cgcttttttg
     2461 ggcacttttg ccaccacgtt cacataggga tcaaccacct cagcgatgtc gtaccgcatg
     2521 gccgtcccca gcacggacgc cacttcggcc gcgttcaact tgtagctgtc ctccagccag
     2581 cgtgccatca tcgtcgtcgc aatctgaaac gcctctgtca gtgaaccggc gatgcccatc
     2641 gccatcaggt actcgtcgtc ctcagcacgc ggcatcgcca gggacttgcc cgcaatcaca
     2701 tccacggaga attcgatgtc catcgacgtt tcgagcgcgt tacctgtcag ttcgccgtca
     2761 ccctgtgccg cgtgcccgtc acccatgaac aatagcgccc cctgctggga tacgggcaaa
     2821 tacagcgtca cgccttcgcg caaccgatta taatccatgt tgccgccaaa gctccccgga
     2881 tagccagacc ggaaaaccat gtggcccggc ggcgccacgc ccacacaccc caggatcggc
     2941 cgcaggggca ccgtgtagtt tttcaatgcc tcggtcggtt tggccaacct ggcaattcct
     3001 ttttcgcgat ccagcaccca gttgctatcg aagttctcca ccggcttcct gtttcgtacg
     3061 tagcctggtg tgatcgcgct ggccgcgagg ctaccagtcg agaaggccgt gtcccgattc
     3121 agcctgatgc gcttcaactt cacaacgagc gtatcgccgg gcagcgatcc ttcaatgtag
     3181 aacgggccgg tctgcggatt cccccccaac gatctccgca caccctgcgc gtccacgccc
     3241 ccggcatcca cgctccaggt ctttacggaa tctcctggat aaatgtggag gaccggcgga
     3301 atcgccccag aaaacacccg gtggaactct ttcggctcaa acacgtgctc tctgggcgcg
     3361 tcggtcgggc gaaccggaat ccgtgtcgcc ttccatctcc cagtcatggt tcccatcgtg
     3421 aagtcgcctg acattgtacc ccgctcgacc gtgccggtca ttttcatggg ggtctcgtct
     3481 ttttcacgct gctctagctc catcttccca tcgcgaattt tgccctccag tttcatgcca
     3541 tcaagcacga cggccagttg gtctcccacc gctcgaaacg tcattcgatg gtaatccgtc
     3601 tcgccgtata gctcggcggc gacaacccag tcgccggcca ggtcggctgg cgtgatgctc
     3661 tggccttcca gcgccgccgg ccccaacaac gtgaggatga cccccaagaa tggttttcgc
     3721 atcgtttgct gacctccaga ccatgtattc acttatgact tgcacatgat cattt
//
LOCUS       DATAQL010000231         7057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4755984, whole genome shotgun sequence.
ACCESSION   DATAQL010000231 DATAQL010000000
VERSION     DATAQL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4755984"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..2507
                     /locus_tag="VHS97_03960"
     CDS             120..2507
                     /locus_tag="VHS97_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3447378.1"
                     /translation="MKTDLQSITGHLCLTGAGLSCLVLLALPIMAAAGPVRTGEQIYR
                     QQCASCHGSIGEGTDDHYPRPLVGERSVASLSRLIAKTMPEDAPGECVGEDADKVAAF
                     IYESFYSKTAPGRTKFQVPRIELSRLTVREYKTAIADLLGSFQAPGRWDDQRGLKGEY
                     SSRSRRRRDGNGGGSAFNRVDPEIRLDFGTGSPIPEQNALADVAKSWQRFPVLVVPLS
                     TFRGYSQDFRINWQGSLLAPETGEYEFLVRTENATRLWVNNMVRPLIDAGVKSGSDTE
                     YRGSVYLLGGRVYPLKLELSRTKEKTSSIALEWKLPRRAYEVIPLRNLSPNSFPETFV
                     LQTPFPPDDRSVGYERGTSISKAWDQAATDASIEVAGYIAAHLKELAGVGDEAADREP
                     KFREFCRLFAERAFRRPLTDEQKTFFIERQFKEAGNPQVAIKRVVLLVLKSPRFLYRE
                     IGNSRPDGYDVAARLSFGLWDSLPDRALLDAAAASQLGNREQVISQSERMVGDLRARS
                     KLREFFQQWLKVEQVPDLSKDPKRYSQFNEAIASDLRTSLDLFLDDVIGSDTADFRQL
                     LCADYVYLNGRLAQFYGADLPPDAPFQKVALEPRERAGILTHPYLMSSFAYTASSSPI
                     HRGVFIARSVLGRALRPPPEAVAPLAPDLHPDLTTRQRVTLQTKPETCVSCHGMINPL
                     GFTLEHFDAVGRYRSEEKGRPVDATGSYETRKGEIVKFGGVRDLATFLANSDETHTAF
                     VQQLFHYLVKQPVRAYGPHTLTDLKRFFVANNFNLHKLMVEIMATTALTSQGVKS"
     gene            2585..3904
                     /locus_tag="VHS97_03965"
     CDS             2585..3904
                     /locus_tag="VHS97_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3447379.1"
                     /translation="MAYRSNRREFIRDLGLSAAAFPFILNLPSLGFANQQRRKQRLVI
                     MFSPNGVVPPAFWPDEEGPSFKLKECLTPLEPFKDRTLVLHGVCDQLRGDGDNHMRGM
                     GCLLTGIELFPGNIQGGSDTPAGWAKGLSIDQEIKNALQSKSETRTRFGSLEFGVMVP
                     DRADTWTRMVYAGPNKPIAPLDDPYQMFAKLYGRKKDQESLKSILDDLEEDLQKLGKV
                     VSSDDRRLLEEHATFVREMEHDLDEPPSRDVGHAVPELEPGVKYDAVNMPKISKMQID
                     LMVNSFAADFSRVATMQFTNSVGGARMKWLGVTEGHHELSHNPDSDQKSQEKLTKINK
                     WYCEQMAYLVKRLAETPEPGGSGNLLDNTLVIWTNELGKGNSHTLNDIPFVLVGNGLD
                     FRMGRSLKFKKVPHNRLLLALANGMGHHIEKFGNPNLCGGGVLTGLT"
     gene            complement(3932..5023)
                     /locus_tag="VHS97_03970"
     CDS             complement(3932..5023)
                     /locus_tag="VHS97_03970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3447380.1"
                     /translation="MGTFAIGLTDHLEGPREQPSSVIFDEVADLVRLADQLGVEYAWF
                     SEHHGHAHCGHLPTPLLFALHMASQTQNIRLGTAIICLNLHHPLDVAEQVAVADVLTK
                     GRIAVGFGSGSTPEEFRLFGLAETDNEERHGRFAEALQLICSIWCANRTKADTGTSES
                     ARYFPVPPHHSLPASSKDLLRRSWVAVNSVGSAKIAGRFNFNILFSHLRTPDQYRQFR
                     IAYRAAGGVGQIAANRPIFVGPDDESAFARVEPALRTLWRRFRQEGKIPAETVEPDRP
                     QDLCAHPINFIVGGPVSVARQLLGLYEQVPFDVANVEVRWAGLSHELVRDSLRRLMED
                     VMPILEHDKFGQRGVRTQERRSATEPESR"
     gene            complement(5106..6560)
                     /locus_tag="VHS97_03975"
     CDS             complement(5106..6560)
                     /locus_tag="VHS97_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447381.1"
                     /translation="MISNRRINHSLFPLNRRAFLGRYAGAIGTLALTYLFEQERVLPA
                     NTTNAGEGASGKGTIGSDRRARARSVISLFQHGGPSQIDLFDPKPALTKHDGEPYPGQ
                     LEIHFDKQAGKLLGSPFRFRPHGQSGMVLSELVPHLSGIVDDLTLVRSMTTESVDHET
                     ALRLIHTGKILAGRPTWGSWVAYGLGTENQNLPAYVVLSDPDGLPVDGVRNWSGGWLP
                     AIDQGTPFRSGNAAVLNLKRPPNITAEARDYQLRFLEELNHAHSKRHPGDTELEARIA
                     NFEIAAAMQTAVPEALDLSMESMATRRMYGLDNPTTHDYGTRCLIARRLVERGVRFVQ
                     LFLSSQPWDTHSKNAQTLKSLCARTDQPSAALVRDLKQRGLLDETIVLWTGEFGRLPI
                     SQGKDGRDHNRHGFSLWLAGGGFKPGYVHGATDEFGYKAIDDMVTVHDLLATLLHALG
                     LDHRKLTYPHEGRLDSLTDAEVTQAKVVPQLLAV"
     gene            complement(6566..>7057)
                     /locus_tag="VHS97_03980"
     CDS             complement(6566..>7057)
                     /locus_tag="VHS97_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3447382.1"
                     /translation="PYVPTHRDGSGEVVVTESAEGATRRSIYLQQRRTEITSLLDLFD
                     APSIVTTCTRRVPSTIPLQSLSLMNSDFMVNGAGKLATRLEHACTTGPDGCVDHDACI
                     ERAFLLVIGRQPAADERASARRFVATQPSRYPGLVEAECHHRALADFCQMLLASNAFL
                     YVD"
BASE COUNT         1440 a         2074 c         2097 g         1446 t
ORIGIN      
        1 gttgccgcta acggattctg ggtctcattg gcccggagct agcggttaat ctcgcctggc
       61 cgagtgtcga acctggtatt ggcacgctaa agtccaaggc cctgatggtt ggctttccca
      121 tgaagaccga tctgcaatcg atcaccggtc acctctgcct taccggggct ggattgtcgt
      181 gtctcgtgct cctggcttta cccataatgg cggctgcggg tcccgttaga actggtgagc
      241 aaatctaccg tcagcaatgt gcctcctgtc acggctcgat aggcgaaggc acggacgatc
      301 attatccgcg gccactcgtg ggagagcgat cggtggccag tctatcgcgg ttaatcgcca
      361 agaccatgcc tgaagatgcc cctggggagt gcgtgggtga ggatgccgat aaagtcgcgg
      421 cgttcattta cgagtccttc tactccaaga ccgccccggg gcgcaccaaa tttcaagttc
      481 cgcgaatcga gctctctcgc ctgaccgttc gcgaatacaa aaccgcaatc gccgacctgc
      541 ttggcagctt ccaagcccct ggacgctggg acgatcagcg tggcttgaag ggagaatact
      601 cgagccgatc cagacgcagg cgtgacggca atggtggcgg gagcgcattc aatcgagtgg
      661 acccggagat tcggctcgat ttcggcacag gcagccccat ccccgaacaa aacgcactcg
      721 cggatgtcgc gaagagctgg cagaggttcc ccgtgctcgt ggttcctctt tcgaccttca
      781 gggggtactc gcaagatttc aggattaact ggcaagggtc gttgcttgct cccgagaccg
      841 gggaatacga attcctcgtc cgaaccgaga atgccacgcg gctatgggtt aacaacatgg
      901 tccgaccctt gatcgacgcc ggggtcaagt cggggagtga caccgagtat cgagggtcgg
      961 tctatctttt ggggggacgg gtttatccgc tcaagcttga gctgtctcgt accaaggaaa
     1021 aaacgtcctc gatcgcactg gaatggaagc tgccgcggcg agcctatgag gtgattccgc
     1081 tgcgcaatct ctctccaaac tcttttcccg aaacatttgt tttgcaaacg ccgtttccgc
     1141 ccgacgatcg cagcgtcggc tacgagcggg gcacgtcgat ctccaaggcg tgggaccaag
     1201 ccgccacgga cgcgtcaatc gaggttgcgg gttacattgc cgctcatttg aaagagctag
     1261 cgggcgttgg ggacgaggca gctgaccgtg agcccaagtt ccgcgaattc tgccgcttgt
     1321 tcgcggagcg ggcatttcga cgcccgttga cggacgaaca gaagacgttt ttcatcgagc
     1381 gccaattcaa agaggccggc aacccgcagg tagccatcaa gcgggttgtc ctcttagtcc
     1441 tcaaatcacc ccggttcctg taccgtgaga tcggcaacag ccgccccgac ggctatgacg
     1501 tggcagcccg gctctcgttc gggctttggg actcactgcc cgaccgggcg cttctcgatg
     1561 ccgcggcggc cagccagttg gggaaccgcg aacaagtcat cagccaatcc gaacggatgg
     1621 tcggtgatct ccgagcgcgc tcaaagctac gcgagttctt tcagcaatgg ctcaaggtcg
     1681 agcaagtacc tgatctttcc aaggatccca agcgctactc acaattcaac gaggcgattg
     1741 cctctgatct gcgcacgtca cttgacttgt ttctcgacga tgtgattggc agtgacacag
     1801 ccgatttccg ccagctcctg tgcgcggatt acgtttactt gaatggccgg ctagcgcagt
     1861 tttatggtgc cgatctgcct ccggacgcgc cgtttcaaaa ggtcgctttg gaaccgcgcg
     1921 aacgcgcagg catcctgact cacccctacc ttatgtcgag ctttgcctac acggccagta
     1981 gctcgcccat tcatcgtgga gtgttcatcg cgcggagtgt cctgggacgg gcgcttcgac
     2041 cgccccctga agccgtagcg ccgcttgcgc ctgacctcca ccccgatctg acgacccgcc
     2101 aacgcgtcac actccagacg aaaccggaga cgtgtgtgtc gtgccacggc atgatcaatc
     2161 cgctcggatt taccctggag catttcgatg ccgtcggccg atatcgaagc gaggagaaag
     2221 gtcggccggt ggacgcgact ggctcgtacg agacccgaaa gggcgaaatc gtcaaatttg
     2281 gcggagtccg agatctcgcg acttttctcg ccaacagtga cgagacacat accgccttcg
     2341 tccagcagtt gtttcactac ttggtcaagc agccggtccg tgcttatggc ccccacacgc
     2401 ttaccgacct caagcggttc ttcgtggcca ataattttaa cttgcacaag ctaatggttg
     2461 aaatcatggc gacaacggct ttgacgtctc aaggggtgaa gtcgtaaact cttggtgtcg
     2521 gtcaatccgt tcatagccgc actcgccccg cccgcttctg ccaaccgcct tccggagaga
     2581 tgaaatggcc taccgaagta accgtcgcga gttcatccga gacctgggct tgagcgcagc
     2641 tgccttcccc ttcattttga atttgcctag cctggggttc gccaatcaac agcgtcgcaa
     2701 gcaacggctg gtcatcatgt tcagccccaa cggcgtggtc ccgcccgcgt tttggcccga
     2761 tgaggaaggt ccgtcgttca agctgaaaga gtgtttgacg ccgctggagc cgttcaagga
     2821 tcgcacgctg gttctgcacg gtgtctgcga ccagcttcgg ggtgatggcg acaaccacat
     2881 gcgcggcatg ggctgcctgc tgacggggat cgagctcttt cccggcaaca tccagggtgg
     2941 ttccgacacc ccggcgggat gggcgaaagg actctcgatc gatcaggaaa tcaaaaacgc
     3001 gctgcagagt aaatcagaaa cgcgtacccg gtttggctcg ctcgagtttg gcgtgatggt
     3061 tcccgatcgc gcggatacgt ggactcgaat ggtctacgcc ggtccgaaca aaccaatcgc
     3121 cccgctcgac gatccttacc agatgtttgc caagctgtac gggcgcaaaa aggatcaaga
     3181 aagcttgaag agcattctcg acgatcttga ggaagatctg caaaagctag gcaaggtcgt
     3241 cagctccgac gatcgaagac tgttggaaga gcatgcgacc ttcgtgcgcg agatggagca
     3301 cgacttggac gagcccccca gccgcgacgt tggccatgcc gttcccgagc tcgagccggg
     3361 agtaaagtac gacgccgtca atatgcccaa gatcagcaag atgcagatcg acctcatggt
     3421 caatagcttc gcggccgatt tctcccgcgt agcaaccatg cagttcacca actcggtagg
     3481 cggtgcgcgg atgaaatggc tcggcgtcac cgaggggcac cacgagttgt cgcacaaccc
     3541 cgatagtgac cagaagtctc aggaaaagct gaccaagatc aacaaatggt attgcgagca
     3601 gatggcctat ctggtgaagc ggctggcaga gacgcccgag ccgggaggta gcggcaacct
     3661 gctcgacaac accttggtga tctggaccaa cgagctcggc aagggcaatt cccacacctt
     3721 gaacgatatt ccattcgttc tcgtggggaa tggcctggat tttcgcatgg gccgctcgct
     3781 caagttcaag aaggtgcccc acaaccggct cctcctcgcc ttggccaacg gcatgggtca
     3841 ccacatcgag aagttcggta acccaaacct ctgcggcggc ggggtgctga cgggtttgac
     3901 ttgatgtgag aatcgattga agtcaaatcc gttatcttga ctccggctca gttgcactcc
     3961 gtcgctcctg agtccggacg cctctctgcc cgaacttgtc atgttcgagg atcggcatca
     4021 cgtcctccat caatcgccga agactgtcgc gcaccaactc gtgtgacaac ccggcccacc
     4081 gcacttccac gttggcgacg tcgaatggca cctgttcata tagcccgagg agctgccgcg
     4141 cgacggacac ggggccaccg acgataaaat tgattgggtg cgcgcacaga tcctggggcc
     4201 gatccggctc gaccgtttcc gcgggaatct ttccttcttg gcggaatcgt cgccagaggg
     4261 ttcgtaacgc cggctcgaca cgagcaaagg ctgactcatc gtcggggccg acgaagatcg
     4321 gtcggttggc tgcgatctgt cccactccgc cggctgccct gtaggcaatg cggaactgcc
     4381 ggtattggtc cggcgttcgg aggtgcgaga acagaatgtt gaaattgaac ctgccagcga
     4441 ttttcgccga gcctacactg ttgactgcga cccacgaacg gcgcagtaga tctttcgacg
     4501 aggccgggag tgaatggtga ggtggaaccg gaaagtagcg agcggactca gaggttcctg
     4561 tatctgcctt ggtgcggttc gcgcaccata tcgagcaaat aagttgaagc gcttccgcga
     4621 aacgtccatg ccgctcttcg ttgtcggttt ctgcgagacc gaaaagacga aactcctcgg
     4681 gggtactacc actgccgaat cccacggcta tccgaccttt ggtcaataca tccgcgactg
     4741 ctacctgttc tgcgacatcg agtggatggt gcaggttgag acagatgatt gccgtgccca
     4801 ggcggatgtt ttgagtctga ctggccatgt gcaacgcaaa aagcaacgga gtgggcagat
     4861 gcccgcagtg cgcatggccg tgatgctcgg aaaaccacgc atactccacg ccaagctgat
     4921 cggctagccg gaccaggtcg gcgacttcgt caaagatcac cgacgatggt tgctctcgtg
     4981 gtccttcaag gtggtcggtc agcccgatcg cgaacgtgcc cactatccat agccgtccgc
     5041 ttgcggacgt cctccaagct ccgcggataa caatcatgcc actctagttg tgcggcagat
     5101 tccagtcaaa ctgcgagcaa ttgaggcacg accttagcct gagtgacctc ggcgtcggtc
     5161 aggctgtcga ggcgcccttc gtgcgggtag gtcagcttgc ggtgatcaag ccctaacgcg
     5221 tgaaggagcg tggccaggag gtcatggacc gtcaccatgt cgtcgatggc cttgtagccg
     5281 aactcgtcgg tcgcgccgtg tacatagccg ggcttaaacc ctccgccggc cagccaaagt
     5341 gagaagccgt ggcggttatg gtcgcgaccg tctttgcctt gggagatggg cagccggcca
     5401 aactcgcctg tccagagcac gatcgtctcg tccagaagcc cgcgctgttt aaggtccctg
     5461 accaaggcag cgctgggctg gtcggtgcgt gcacagagcg atttcaaagt ctgggcgttc
     5521 ttgctgtgcg tgtcccaggg ttgactgctt agaaatagtt gcacaaaccg tactcctcgc
     5581 tcgaccaaac gccgggcgat cagacagcgt gtgccataat cgtgggtggt tggattgtcg
     5641 aggccgtaca tgcggcgggt ggccatcgat tccatcgata gatcgagcgc ctcgggaacg
     5701 gctgtctgca ttgccgcggc gatctcaaag ttcgcgattc gcgcttccag ctcggtatcg
     5761 cctgggtgcc gcttggaatg ggcgtggttt agttcttcca ggaagcgaag ctgataatca
     5821 cgagcctcgg ccgtgatgtt tggcggccgc ttcaggttaa gcaccgcggc gttgcccgag
     5881 cgaaaaggtg tcccttggtc gatcgccggt agccagccgc ccgaccagtt gcgcacgccg
     5941 tcgaccggca gaccgtccgg atccgacaag accacataag ctggcagatt ctggttctcc
     6001 gttccaaggc cgtacgcgac ccatgagccc caggtcggcc gtccggccag gatcttgccg
     6061 gtgtggatca atcgcagcgc tgtctcatga tcgactgact cggtcgtcat cgatcgcacc
     6121 agggtcaagt cgtccacaat cccggacagg tgcgggacca gctcgctgag caccattccc
     6181 gattgaccat gtggacggaa ccgaaacggc gaccccaata gtttgcctgc ttgtttgtcg
     6241 aaatggatct cgagctggcc cgggtaaggc tcgccatcgt gttttgtgag cgcgggtttg
     6301 ggatcaaaca gatcgatctg gctcggcccg ccgtgctgga acaggcttat caccgaccgc
     6361 gctcgagccc gacggtcact tccaatcgtt cccttgcccg aggcgccttc gcccgcattc
     6421 gtagtgttcg ccgggagcac gcgctcttgc tcgaacaggt aggtaagggc caaagtcccg
     6481 atcgctccgg catatcggcc cagaaacgcc cggcgattca aagggaacag cgagtgattt
     6541 atccgacggt ttgagatcat cagattcagt cgacataaag aaaagcgttg ctggcaagta
     6601 gcatctggca gaaatcagcc agggcacggt gatgacactc agcttcaacc aggccggggt
     6661 atcgcgaagg ttgcgtcgcc acaaatctgc gagccgaggc tcgttcatcg gcggctggct
     6721 ggcggccaat tacgagtaga aaagcccgtt caatgcaggc gtcatgatcg acacagccat
     6781 cggggccggt cgtgcacgcg tgctcgagcc tcgttgcgag tttccccgcc ccgtttacca
     6841 tgaagtcaga attcatcaag ctgagcgatt gcaaagggat cgtcgacggc acgcggcggg
     6901 tgcaggtcgt gacgatcgag ggagcgtcga acaggtcgag caggctggtg atctccgtgc
     6961 gccgctgctg gagatagatc gagcggcggg ttgcgccttc ggccgattcc gtgacgacga
     7021 cctcaccgga cccgtcacga tgggtaggta cgtatgg
//
LOCUS       DATAQL010000232         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1550560, whole genome shotgun sequence.
ACCESSION   DATAQL010000232 DATAQL010000000
VERSION     DATAQL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550560"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VHS97_03985"
     CDS             complement(<1..744)
                     /locus_tag="VHS97_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3447383.1"
                     /translation="MAFYDGLFNQLGLREWCDASGSTGPISIAELLKKSKEADAGGQS
                     LAYAPFPSLDALHDACRAVPRTRDLTSGIADSFLYVRVPLKTVLDPLTQPLSWLLDFS
                     LKVVEVVPWWALVIALLLLVYFASRSWPILALVAAILFLLGFIDHLQYALQTMAIVFS
                     CTFIGILVGIPIGILMAKSNRLERLSLPVLDMLQTLPPFVYLIPLIFLFSVTESKLYG
                     IAIILYAVVPVIRLTNLGIRMVDGSVGEAA"
     gene            complement(861..1832)
                     /locus_tag="VHS97_03990"
     CDS             complement(861..1832)
                     /locus_tag="VHS97_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3447384.1"
                     /translation="MKSILAAVGMIGAGFLASVSSANAACGTVTITEMNWPSSAVVTA
                     VSSFLMEQGYGCTVRKVPTSTVPALTSLAETGQPDILTEVWPGVADIYYKLEKDGKIV
                     KVGDVISDGGVDGWWIPDYLAQAHPELKTLGGILANPKLVGGRFNNCPVGWGCRTSND
                     NLIKAFELEKHGIEVFNHGSGETLAASIASAYTAKEPWFGYYWGPTGVLGKYRMVEVD
                     LGPVDKDKAKCNATDNCPTPGKTGWPKATVLTTMSKPFHDKNPELVALMSNVSFSNEI
                     MDELLAWQDTKKATADETAVYFLTKYKNIWPNWLSEDAKAKVSAIVK"
BASE COUNT          466 a          609 c          649 g          433 t
ORIGIN      
        1 cgcagcctcg ccgacgctac cgtcgaccat cctgatgccg aggttggtga gccggattac
       61 tggcacgaca gcgtatagga tgatcgcgat cccgtagagc ttcgactcgg tgaccgagaa
      121 gaggaagatc aggggaatga ggtagacgaa ggggggcagc gtctgaagca tatcgaggac
      181 aggaagggag agcctctcca gccgattgct cttggccatc agtatgccga tgggtatgcc
      241 aacgaggatg ccgatgaacg tgcaagaaaa gacgatcgcc atcgtttgga gcgcatattg
      301 gaggtggtcg ataaaaccaa ggaggaacaa aatcgctgcg accagagcaa ggataggcca
      361 tgaccgggac gcgaaataaa caagtagcag gagcgcaatc accagcgccc accacgggac
      421 gacttcgacg accttgagcg agaaatccag gagccaagac agcggttgcg tcaggggatc
      481 gagcacggtc ttcaacggga cgcggacgta gaggaagctg tccgcgatgc cggaggtcag
      541 atcccgggtg cgcggcacgg cgcgacaggc gtcatgcaac gcgtcgagcg aagggaacgg
      601 ggcgtacgcc agcgactgcc cgcccgcatc cgcttctttg ctcttcttga gcagttcggc
      661 aatgctgatg gggcctgtgg agcccgaggc gtcgcaccac tcacgaagcc cgagctgatt
      721 aaatagtccg tcgtagaaag ccatgagggg ccttccgtgt ggagttgaat tgatgcggcg
      781 cgaccccagc ccggaaactc aaggtcaaga aaacgaacaa tgggtccgcg gacgatatca
      841 cgcccgacgg acccaacaca ttacttgacg atggcgctga ccttcgcttt ggcgtcttca
      901 gacagccagt tcggccaaat attcttgtat tttgtcagga agtacacggc tgtttcgtcg
      961 gcggtagcct tcttggtgtc ctgccaggca agcagctcgt ccatgatctc gttgctgaag
     1021 ctcacgttgc tcatgagcgc gaccagttcg gggttcttat cgtggaaagg cttcgacatc
     1081 gtggtcaaga ccgtggcctt cggccagcca gtcttgcctg gggttgggca attgtcagta
     1141 gcgttgcact tcgctttgtc cttgtcgaca ggccccaggt cgacctccac catcctgtac
     1201 ttgcctagga cacccgtcgg accccaatag tagccgaacc aaggctcctt cgccgtatag
     1261 gccgatgcga tggacgccgc aagcgtctcg ccggagccgt ggttgaagac ttcgatgcca
     1321 tgtttttcca attcgaaggc tttgataagg ttgtcattcg aggtgcggca gccccagccg
     1381 acgggacagt tgttgaaccg gccgccgacg agttttgggt tcgccaagat ccctcctagc
     1441 gtcttcagtt cgggatgggc ctgcgcgaga tagtcgggaa tccaccaccc atcgacgccg
     1501 ccgtccgaga tcacatcgcc gaccttgacg atcttgccgt ccttctcgag cttgtaatag
     1561 atatccgcga caccgggcca cacttccgtc aggatgtcag gctgcccggt ttcggcaagc
     1621 gaagtgagcg ctggaaccgt cgaggtgggc accttcctca ccgtgcagcc gtaaccctgc
     1681 tccatcagga agctcgaaac ggcggtcacc acggcggatg aaggccagtt catttccgtg
     1741 atggtcaccg tgccgcacgc cgcgttcgcg gaggatacgc tggccagaaa gcccgcgccg
     1801 atcatgccga ctgctgcaag aatagatttc atgccattcc cctttggcga gttgagcctc
     1861 gaccattcgc ggcgagcttg actgttgcct ctgggcggct cgcgatcagg tcgctttccc
     1921 ggctgattaa gcgggaggtt actgcccggg gcaatcaagt gacaagttgc ttaattccta
     1981 cgtaatgagt aagctgaagt ttatgcaaaa acttcgcaac aaactgccgc cgccgaattt
     2041 tcttataact ttcgaagccg cggggcgtca gcttagcttt accaaagcgg catcggaact
     2101 gaacgtttcg cgtgtcgcgg tcagccagca gatccgggcg ttggagaaat ttctcgg
//
LOCUS       DATAQL010000233         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3993432, whole genome shotgun sequence.
ACCESSION   DATAQL010000233 DATAQL010000000
VERSION     DATAQL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3993432"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1718
                     /locus_tag="VHS97_03995"
     CDS             198..1718
                     /locus_tag="VHS97_03995"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447385.1"
                     /translation="MTSGFRGKLMICSLLSFLMTLAAAGWSLAGLTQGRRPDLAVDSL
                     EYVVVRRADLDTTLLAGGDLQPAKQATVTCEVEDVTDSDGTMVLSVIENGAPVKKGDE
                     ICRLDSSAIEELARKEEILVNQARAFYVKARLDFETAKIALQQYQEGLVTQWTKEFES
                     RIALGRSDVKRQEDRLAWVEAMLAKGYLAVGQFLSERQSLARLRHELTKTEGEYELFR
                     RFQVPKEIHSLRGQIQTAEIGFRLEADRLKAEEDELAYLRKQIENCTIRAPQDGVVVH
                     AHRNRWWARPLEPGTRVYQEQALFWIPDLSQMEVDVSVHESMGPRVRAGMKAKVKIAS
                     RADQAVPGRVVAVNLLPTSNWKEWDENLKHFFVRVRLDEKPAKALPYMSATVEIDTGR
                     VSNALFIPVEAMAVVDGQQLCYVVTDDGLERRAIKTRRATRDVVEISAGLQEGERVLS
                     RSLNVEDPAVRGTSHRSGREIARNQSVSPRMSEARARSTPRSIDRQIPAAGDRSTS"
BASE COUNT          425 a          581 c          643 g          396 t
ORIGIN      
        1 cagatctttc gccgttcatt ctcgcgcgac gggccactcc acttctgacc tggagccgcg
       61 ctcgttcgtt gagttcgttg gcgatccctt gacaatcgtg ggactcatgt cttacacttg
      121 tcatcgatag gggagcgctc gatgttccgg ggctcccgca taagaactga tccgcatgcc
      181 aaggggtgag tcgcttgatg acgagcgggt tccgcgggaa attgatgatc tgctcgctgc
      241 tctctttctt gatgaccctg gcagcggcgg gttggtcgct ggccgggctc acgcaagggc
      301 gacgaccgga tttggcggtc gactcactcg agtatgtcgt cgtgcggcgt gccgacctcg
      361 acacgacgct tcttgccggc ggtgatctcc aaccggccaa gcaggcgaca gtcacatgtg
      421 aggttgagga cgtcaccgac tcggacggaa cgatggtcct gagcgtgatc gagaacggcg
      481 cgcctgtcaa gaaaggggac gagatttgcc ggctcgattc ctcggcaatt gaagagttgg
      541 ccaggaagga agaaatcctg gtcaaccagg cgcgggcatt ctacgtgaag gcccggctgg
      601 acttcgagac ggccaagatt gcgctgcaac agtatcagga gggtctggtt acccagtgga
      661 cgaaggagtt cgaatcacgt atcgcactcg gtcgatccga cgtaaagcga caggaagacc
      721 ggctcgcctg ggtggaggcg atgctggcca aaggctatct cgccgtaggc cagttccttt
      781 ccgaacggca gtccctcgcg cgcttgcgcc acgagctgac aaagaccgag ggtgaatacg
      841 agctcttccg ccggttccag gtccccaagg agattcattc actgcgaggt cagattcaga
      901 cagcggagat cggcttccga ctggaggccg accggctcaa agccgaagag gatgagctcg
      961 cctatcttcg aaagcagatc gagaattgca ccatccgagc accgcaagac ggtgtggtcg
     1021 tgcacgctca ccgcaaccgc tggtgggcgc ggccgctcga gccaggtacg cgggtctacc
     1081 aggaacaggc gttgttctgg atccccgacc tgagccagat ggaagttgac gtgtcggtcc
     1141 acgaatcgat gggccctcgg gtacgcgccg ggatgaaagc caaggtcaag atcgcctctc
     1201 gcgccgacca ggctgttccg ggccgagtcg ttgcggtcaa cctgctgccg acctcaaact
     1261 ggaaagaatg ggacgaaaat ctgaaacact tctttgtacg ggtgcgactg gacgagaaac
     1321 cggcgaaagc cttgccgtac atgtcggcca ccgtggagat tgacacgggc cgcgtctcga
     1381 acgcactttt catccctgtc gaggcgatgg ccgtggtaga tggtcagcag ttgtgctatg
     1441 tcgtcacaga cgacggcctg gagcggcggg cgatcaagac gcgccgcgcg acgcgagacg
     1501 tggtagagat ctccgcgggc ctgcaagaag gggagcgagt cctttcacgg tcactcaacg
     1561 tcgaggatcc cgcggtccgc ggcaccagtc atcgttctgg ccgcgaaatc gcgaggaacc
     1621 aatcagtatc gccacgcatg tcggaagcaa gggcgcggtc gacgccgcgg tcgatcgatc
     1681 ggcagattcc cgccgccgga gatcggtcga cttcgtgaac ttgattccgg cgcatgatgg
     1741 ttttcatcgc tgatgcggcc atggtcgtgg aaaccaaatc gtctccgttc atggtctcgc
     1801 agcgccttct agcccgtcgg ttgggcatca ctcaacccag gtcgactcgc cctcgacggc
     1861 tcatttccgc gccactcgac ctagcaaact tgcagggctc ctacaaaatg tagtaaaacg
     1921 gtactacagt atgtagaagg ctgtaagggg gatttcgcac gaagggtgga gaatactctt
     1981 tcagacgagg agcgcggctc ataaagtggt tcacataaac ctcggtcgaa aggatcggat
     2041 ctagc
//
LOCUS       DATAQL010000234         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_940134, whole genome shotgun sequence.
ACCESSION   DATAQL010000234 DATAQL010000000
VERSION     DATAQL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940134"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1421)
                     /locus_tag="VHS97_04000"
     CDS             complement(114..1421)
                     /locus_tag="VHS97_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447386.1"
                     /translation="MSKSRTKGRRPESRAVRPLLDGRLEDRMLLSSAAARTQRDLAIS
                     HFLLTHPAPKNAYSLHKPPQLAKLAPQFHGRTRRRSGVVATQTARGGQNVEINAGGSH
                     YMVSLAYTSNTLATNVAEGQNGQLGASNLTDASQSVLAANATFPQPIGTVRAYAMSNH
                     RVGIIVDGSTQNTELTINPLGQQQRKGFAHSFAYGAANLGHILNVGQLTVSSGQIGDI
                     EGFQTADLSGPLVVSGNTQIDRIALDAILPGGSITTGSDVNTLDVYNGITLTGTNINI
                     GRDVNLLNVGGNISLSNGSQFNIARNLGLVSQPPKGTGTGTNILSLNFVTVSNSIVSV
                     TIPAVGAYIQGSVIVNPGSLFNIGGNIFNTFYVEGSTTAGFTPSSNPGTPTATNPTVV
                     PSLGTLSRLTVENGTPAPLLQSALSQFGSSTDTGYVTSLGGFA"
     gene            complement(2124..3431)
                     /locus_tag="VHS97_04005"
     CDS             complement(2124..3431)
                     /locus_tag="VHS97_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447387.1"
                     /translation="MALEKELVRVAMNHDGVINRRHFVQTIALGTAGLAGSATIPISF
                     TDWMAVHAADLRKRQMACILLWMAGGPSQLETFDPKPGTEHGGETKAIPTAVPGVSIA
                     EGWGHTAKVMNDIAIVRSMTNKEGNHQRATYQLHTGYVPSATIKHPNIGCSMVSEMGE
                     SQFDLPHIVSIGGPTTGAGFLGAALEPFVVQNPHQPPENTQPKVGADRFRRRMGLLER
                     IEGAGFERTGGGDRVRDHKALYRQTAKMILSPHMRAFHLDEEPDPLRDAYGRTPFGQG
                     CLLARRLVQAGVTFIEVRSNGWDTHQQNHEKVGKLADQVDPGFAALVTDLKQRGMLNQ
                     TLVVWMGEFGRTPKINPNAGRDHFPKVFNVALAGGGIKGGQVIGSSNADGSEVKDHPV
                     TVPDLLASLCHSLKVDPAKEMMSPIGRPIKIVDGGNHVSALFG"
     gene            complement(3511..>4450)
                     /locus_tag="VHS97_04010"
     CDS             complement(3511..>4450)
                     /locus_tag="VHS97_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3447388.1"
                     /translation="VGVPERRALAASYVTGQDNPWFAKAYINRIWYALLGEAFYEPID
                     DIGPERTAKAVEVLEPLAEQWQKGGYDIRWLFRLILNTEVYQRRIRSTANEAGKTLFA
                     SSCPSRMRADQVFDALAQALALPLDTSGNIQQPAKPNANNPAPGKNAKQAQASLSLGD
                     PKRAPGQAQTKGAAKKTAAAIGLASQVGKKPGAMLRNGGPRLLVDRLFGVDPSVANED
                     VVGTIPQALFLMNSPLVNDRTKAKPGTALGEILTSAPNDRAALGMLYLRILSRQPTNH
                     EVEICGRYLASVGDRVEAFEDIYWSLINTTEFLTRR"
BASE COUNT          792 a         1334 c         1383 g          941 t
ORIGIN      
        1 ctcctcgatc caattgttcc tccagcacaa acggctcggc acgccgcgag aggttccgcg
       61 gcgcgcctag ccattatcta ttgaacggat ctcaatctca tgtcgcggac ggctcaggcg
      121 aatccgccga ggctcgtcac ataacctgtg tccgtagagc tgccaaactg gctgagcgcg
      181 gattggagca gaggggcagg agtgccgttt tcgactgtga gtcgagacaa agtaccgagc
      241 gaaggaacta cggtcgggtt ggtagcggtg ggggtgcccg gattggagct gggagtaaat
      301 ccggccgtcg tggaaccctc cacatagaac gtgttgaaaa tattcccccc gatgttaaac
      361 aggcttcccg ggttgacgat gacactgccc tggatgtagg cgcccactgc gggaatggtg
      421 acactgacaa tcgaattcga gacagtgacg aagttaagtg agaggatgtt cgttcctgtc
      481 ccggtcccct tgggaggctg gctcacaagg ccgaggttgc gtgcaatatt gaactgcgag
      541 ccattggaca ggctgatgtt gccgcccacg ttgagcaagt tcacgtcgcg gccgatattg
      601 atgttcgtgc ccgtgagcgt gattccgttg tacacatcca gggtgttcac gtcgcttccg
      661 gtcgtgattg agccgccggg caagatggcg tcaagggcga tccggtcgat ctgcgtgttg
      721 ccgctcacga ccaacggacc ggacagatcg gccgtctgga aaccctcgat atcgccgatc
      781 tggcccgagc tgaccgtgag ctggcctaca ttgaggatgt ggcctaggtt cgccgcgccg
      841 tacgcgaaac tgtgggcaaa gcccttcctt tgttgctgcc cgagcgggtt gatcgtcaac
      901 tcggtattct gggtcgagcc atcgacgatg atgcctacgc gatgattcga catggcgtag
      961 gctctgacgg tgccgatcgg ctgcggaaac gtcgcgttag cagccaagac gctttgtgag
     1021 gcatccgtga ggtttgatgc ccccaattgc ccattctgtc cttccgcgac gttggtcgcc
     1081 aaggtgttag acgtgtacgc gaggctgacc atatagtggg agccgccggc attgatttcc
     1141 acgttttggc cgcccctggc ggtctgggtc gcgaccacgc ccgaccgtct ccgcgtcctc
     1201 ccgtgaaact gcggtgccag cttcgccagc tgcggcggtt tgtggagcga ataggcattc
     1261 ttcggcgcgg gatgtgtgag caagaaatgt gaaatcgcca gatccctttg ggtccgggcc
     1321 gccgccgatg acaagagcat ccggtcttcc agccggccgt caagcagtgg acggaccgcg
     1381 cgagattccg gtcgacgacc ctttgtgcga gatttcgaca tctttcgatc ctcctgatcg
     1441 tggccaaccg gcgctcgcga agtgcgcgct atcccgctcg gaccgataac aagaggtcat
     1501 cagccgaaaa gctcgcgaca cttcgctcgc gtcggatcca accgaacgcg cgaagagcgc
     1561 ttcatgggat gagcgcggtt gtttcctgta ccgtcttcgt ttatcggcct gagacccgaa
     1621 gactccgcgc tcaatcctcg cgttcgacgg gatcggagtc gttcagcacc gcttggcgcg
     1681 gatcgcgcga gcatggcggt tgtgccggtt gaatgggtgg ggatgctgtc ggctatcgcc
     1741 ggtccgatcg aggtccagcc gaccagcccg ataacgcaca cagattgcgc agctgctgct
     1801 gacagccagt gggtattcct gccgagaatc cgagagcaat tctccgccct cgtaactccc
     1861 ctctcacaat ccctttccgc actccgtggc gttcgcaggt ttcgccaact tcgtggagaa
     1921 atccccgcgt gacctgtcct cgtgccacgc gcggcactcc ggccttgatc cggatgacaa
     1981 aggccgtgcg aggccgcagc gctgtccaca gtgcgcatgc tactagcgtt gccagcccga
     2041 gcagcaggaa aagcgccacc agttttctaa accgtcacaa ccgtattgtt gaaactcgcg
     2101 tcaatgtatg gctcactgga gatttagccg aaaagtgctg agacatgatt gccgccatcg
     2161 acgatcttga ttgggcggcc gatcgggctc atcatttcct tggccgggtc gactttgagc
     2221 gaatggcaca gagaagccaa gaggtcgggt acggtgacgg gatgatcctt gacctcggac
     2281 ccgtcggcgt tggaagagcc gatcacctgg ccccccttga ttccgcctcc ggccaatgcc
     2341 acgttgaaga ctttggggaa atggtcgcgg cccgcgttgg ggttgatctt gggtgttcgg
     2401 ccgaattcgc ccatccagac gaccagagtc tgattgagca tgccgcgctg cttcaagtcg
     2461 gtgaccaaag cggcgaaccc gggatcgacc tggtcggcaa gcttaccgac tttctcgtgg
     2521 ttttgctggt gggtgtccca gccgttggag cgtacctcga tgaaggtgac gcccgcctgc
     2581 actaaccggc gtgccagcag gcaaccctgg ccgaacggcg tccgcccata ggcgtcgcgc
     2641 agcgggtccg gttcctcatc gagatggaag gcccgcatat ggggcgaaag gatcattttt
     2701 gcggtctggc gataaagcgc cttatgatcg cggacgcggt cgccgccgcc ggtacgctcg
     2761 aagcccgccc cctcgatccg ctcgagcaat cccatccggc gccggaagcg atcggcgccg
     2821 accttgggct gcgtgttctc cggcggctgg tgcgggtttt ggacgacaaa cggctcgagc
     2881 gccgcgccga gaaagcctgc gcccgtggtg ggcccgccga tgctgacgat atggggcagg
     2941 tcaaactggc tttcccccat ttcactgacc atcgagcaac cgatgttcgg gtgcttgatc
     3001 gtcgccgagg gcacgtaccc ggtgtgaagc tggtaagtcg cgcgctgatg gtttccctct
     3061 ttattagtca tcgaccggac aatcgctatg tcgttcatga ccttggccgt gtgcccccaa
     3121 ccctcggcaa tcgatacacc cggcacggcc gttgggatgg ctttggtctc gcccccgtgc
     3181 tcggtaccgg gcttgggatc gaacgtttcg agctggctcg gtccgccggc catccagagc
     3241 aagatgcacg ccatctgtcg cttgcgtaaa tcggccgcgt ggacagccat ccaatccgtg
     3301 aagctgatag gtatggtcgc cgagccggcc aatccggcgg tccccagcgc gatcgtttgg
     3361 acaaaatgcc ggcggttgat cacgccgtcg tgattcatcg ccacccgcac caattctttt
     3421 tcgagtgcca tgagtctttg cctcgctatc tcgtggatct tgccgactgt gaggtcgttt
     3481 cacctgttgt cgattgaaag acgcaagagg tcaacgccgg gtgaggaact cggtggtgtt
     3541 gatcaggctc cagtaaatat cttcaaacgc ttcgacgcgg tcgcccacgc tggcgaggta
     3601 gcggccgcag atttcgactt catggttggt gggctgccgc gacaggatgc gtagatagag
     3661 catgcccagg gccgcgcgat cgttgggtgc cgaggtcaag atttcaccca gcgcggtgcc
     3721 tggtttggcc ttggtccgat cattgaccaa cgggctgttc atcaagaaca atgcctgggg
     3781 aattgtcccc acgacgtctt cgttggccac cgagggatcg acgccgaaga gtcgatcgac
     3841 cagcagccgc ggtcccccgt ttcgcagcat cgcgcccggc ttcttgccga cttgggacgc
     3901 cagcccgatg gccgcagcag tctttttcgc cgcgcctttg gtctgtgcct ggccgggcgc
     3961 ccgtttggga tcgcccagac tcaagcttgc ctgggcctgc tttgcgttct tgcccggggc
     4021 tgggttattg gcattgggct ttgcgggctg ctggatattg ccgctggtat ccaacgggag
     4081 cgccagggcc tgggcgagtg catcgaagac ctgatcggcg cgcatgcgac tcgggcagct
     4141 cgaggcgaat agcgttttgc cggcttcgtt ggccgtcgag cggatccgcc gctggtacac
     4201 ctcggtattg agtatcaagc ggaaaagcca gcgaatgtcg taacctccct tttgccattg
     4261 ctcggccaga ggttcgagca cctccacggc cttggctgtt cgctccggcc cgatgtcgtc
     4321 gatcggctcg taaaaggcct cgcccagaag ggcataccag atccggttaa tgtacgcctt
     4381 ggcaaaccag gggttgtcct gtccggtcac gtaagaggcc gccagcgccc gcctctcggg
     4441 cacgcccacg
//
LOCUS       DATAQL010000235         7580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1398241, whole genome shotgun sequence.
ACCESSION   DATAQL010000235 DATAQL010000000
VERSION     DATAQL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398241"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="VHS97_04015"
     CDS             complement(<1..1732)
                     /locus_tag="VHS97_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX3447389.1"
                     /translation="MATVTLTEKDVRAQAGNLVEMSWDPITRIVGSLGIYTKIDFANR
                     RVAECHSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHATCACYAQNMAFGIRP
                     PNIAECIVNLGEAAEYMFDHNIYQDNLVGVDFCEQMVKETNPGVLDQAERTESPHAKL
                     HGYRTIADIMRALNPFTGSFYREALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPT
                     VQLFTDYIVRLMKYVEFMKKVVPMHDDLFDFFYQALPGYEEVGRRRILLGCWGSFQNP
                     EVCDYTYQNMTKWGRAMYVTPGVVVDGQLVTTDLVKINLGIRILLGSSYYDDWDDSEM
                     FVKQDPLGNPVDRKHPWNQTTLPKPQKRDFKGKYTWVMSPRWYDERTKDHLALDTGGG
                     PIARFWATALAGLVDIGYIKATGHSVKIYLPKTTSLPEVELEWKIPKWSNAIERDRAR
                     TYFQAYSAAAALHFAEQALKELHAGRTQTWTEFKVPDEAIGCGFHEAVRGVLSHHVVI
                     RGGKIANYHPYPPTPWNANPRDIYGTPGPYEDAVQNTPIFEENGPDKFKGIDIMRAVR
                     SFDPCLPCGVH"
     gene            complement(1750..2817)
                     /locus_tag="VHS97_04020"
     CDS             complement(1750..2817)
                     /locus_tag="VHS97_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HEX3447390.1"
                     /translation="MAETAVPYGRKTQRAPSVKEVHVLWITAGLGCDGDSVSITAATQ
                     PSIEDVLLGAIPGLPKVHLHNPVLAYEVGDEFMRFWYQAEEGRLDPFVLVVEGSIPNE
                     KIKKEGYWAALGTDIKTGQPIPTCDWIDRLAPKALAVVAIGTCATYGGIHAMEGNPTG
                     CMGLADYLGWQWKSKAGLPIVNVPGCPVQPDNFMETLLYLLYQVAGLAPMIPLDDQLR
                     PTWLFGKTVHEGCDRAGYYEQADFAHEYGSPKCLVKLGCWGPVVNCNVPKRGWMAGIG
                     GCPNVGGICIGCTMPGFPDKFMPFMDEPPGARLSSTAIGLYGRVIRALRGFTQDTANK
                     EPKWRKRGTELATGYRPATRS"
     gene            complement(2885..3958)
                     /gene="hypE"
                     /locus_tag="VHS97_04025"
     CDS             complement(2885..3958)
                     /gene="hypE"
                     /locus_tag="VHS97_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX3447391.1"
                     /translation="MARLGEFNGTCPLPISSYDRIVLGHGGGGLLSAQLIERLFLPAY
                     GNDVLAALEDQATVSLPGRRGADAPRIAITTDSFVVRPLFFPGGDIGRLAVHGTVNDL
                     AVGGARPLYLTAAFILEEGLAIPQLERIAASMRQACDEANVALVAGDTKVVDRGKGDG
                     VFITTTGVGLVPQGRSLSIRSALPGDRIIVSGTIGDHGVAIMSVREGIEFETVVESDT
                     APLSGLVETMLESCPGIRAMRDPTRGGVSSTLNELAAASHVGVKLDESKIPVRADVRG
                     ACEILGLDPLYVANEGKLIAVVAPGEAEKLVAVMRAHPLGREAEEIGEVVSAHPGMVT
                     LRSLIGGERVVAMLSGEQLPRIC"
     gene            complement(3958..5133)
                     /gene="hypD"
                     /locus_tag="VHS97_04030"
     CDS             complement(3958..5133)
                     /gene="hypD"
                     /locus_tag="VHS97_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX3447392.1"
                     /translation="MKYLTEYRDGAAAEKVAAVIARTVTRPWTIMEVCGGQTHTIVKY
                     GLDAILPPLVELVHGPGCPVCVTPLETIDKAHAIAAQPDVIFCSFGDMLRVPGSRGDL
                     LQQKSRGSDVRIVYSPLDAVNLAAANPHRRVVFFAIGFETTAPPNAMAVALARKRGLT
                     NFSMLVSHVLVPPMMKALLAAPDNRIQGFLGPGHVCTVVGCGDYVPIARDFRVPIVIT
                     GFEPLDLLQGILWTIRQLESGRAEVENPYARAVRRDGNPASRRLLGDVFEICDRKWRG
                     IGLIEQSGYRLRAEYRDHDAERLFEVDHIEAHESSVCISGQILKGLKKPHDCPAFGRE
                     CTPQTPLGATMVSSEGACAAYHAYGRHLEAARLDSTGTLIHPAGPSSLPHELAVGDR"
     gene            complement(5130..5381)
                     /locus_tag="VHS97_04035"
     CDS             complement(5130..5381)
                     /locus_tag="VHS97_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX3447393.1"
                     /translation="MCLAIPGKVVETYREHDVRMGKVDFGGILKRVCLEHVPEANIDD
                     YVLVHVGFALSRIDEAEAKHVFEFLGTMDQLNDLEEPSP"
     gene            complement(5384..>7580)
                     /gene="hypF"
                     /locus_tag="VHS97_04040"
     CDS             complement(5384..>7580)
                     /gene="hypF"
                     /locus_tag="VHS97_04040"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX3447394.1"
                     /translation="SDSLDRFLHELSSSPPPIAQIEEVRWSSRPPRGDPDFRIESSEN
                     ELAGSIFVSADFATCDDCLRELFDHRDRRYRYPFLNCTNCGPRFSIIREAPYDRQRTT
                     MAAFAMCAACRAEYEDPRDRRFHAQPIACPVCGPRLRALDSRGQPIDTDQPLALGIAS
                     LMDKKIVAIKGLGGYHLACLADDAAAVALLRRRKHRDQKPLAIMVKDVESARSLCEIS
                     PAELRILISPRRPIVLLHRHRFARIAERVAPGNPTLGVMLPYTPLHHLILSELGDKPL
                     VMTSGNSSDEPIAYEDEDAVARLAGIADLFLTHDRPIHLRCDDSVTRVVAVTELPLRR
                     SRGYAPAPVELPLSCPMPILALGGQLKATFALGRGRHAFLSHHLGDLEYYEAERAYRQ
                     AVPHYERLFGIEPELVVHDLHPDYATTRYAHDRNPSIARLAVQHHHAHVASCMADNLL
                     DEPVIGVAFDGTGLGTDGAIWGGEFLTGGYGGFRRAAHLRYVPMPGGDLAIREPWRMA
                     ASYLADAGQRDGLLAGRVPAASLTTVRRVIDRRINTPMTSSAGRLFDGVAALLGLRDR
                     VTYEGQAAIELEWLAATASSHDVSSFEFDIAAGTELGSPFTIDTRPLVAAIAAAIGRG
                     QDAAQIARRFHSTLVEVVAQVCARLRQQEDLDVVILSGGVFQNALLTSEVIEHLERDG
                     FRVYRHKNVPPGDGGLSLGQLVIAAATGAPHFTRGTTCADVMAVSAMRS"
BASE COUNT         1448 a         2323 c         2426 g         1383 t
ORIGIN      
        1 tatgaacgcc gcagggcaag cacgggtcaa agctgcgcac ggcccgcatg atatcaatcc
       61 ccttaaactt gtcggggccg ttctcctcga agatcggagt attctgaact gcatcttcgt
      121 acgggccggg cgtgccgtag atatcacggg gattggcgtt ccagggagtg ggcggatagg
      181 gatgataatt ggcaatcttc ccgccgcgga taacgacgtg gtgcgagagg acgccacgga
      241 ccgcctcgtg gaagccgcag ccgattgcct catcggggac tttgaattca gtccaggtct
      301 gagtgcgtcc ggcgtgcagc tctttcaagg cctgctcggc aaagtgaagt gcggccgcgg
      361 ccgaataggc ctggaagtag gtgcgggctc ggtcgcgctc gatggcgtta ctccacttgg
      421 gaatcttcca ctccaattcc acctccggaa gcgaagtggt cttgggcaga tagatcttga
      481 cgctgtgccc ggtcgccttg atgtagccga tatcgaccaa gccggccagc gcggtcgccc
      541 agaagcgggc gatcggtccg ccgcccgtat cgagcgcgag gtggtctttg gtgcgctcgt
      601 cgtaccaccg cggcgacatg acccaggtgt actttccctt gaaatcgcgc ttttgcggct
      661 tggggagcgt ggtctggttc cagggatgct tgcgatcgac cgggttgccc agcggatctt
      721 gcttgacgaa catctccgaa tcgtcccaat cgtcgtagta tgagctcccc aagaggatgc
      781 ggatgccgag gttgatcttc accagatcgg tggtgacaag ctggccgtcg accacgactc
      841 cgggggtgac atacatcgcc ctgccccact tggtcatgtt ctgataagtg tagtcgcaga
      901 cctcgggatt ctggaacgag ccccagcagc ccaggaggat ccgtcggcgg ccgacttctt
      961 cataaccggg gagggcctgg tagaagaagt caaagaggtc gtcgtgcatc ggcacgacct
     1021 ttttcatgaa ctcgacgtac ttcatgagcc ggacgatgta atcggtaaag agctggacgg
     1081 tcgggacggt ccccactcca ccggggtaga gagtcgacgg gtggacgtgc cgtccctcca
     1141 tcagacagaa catctcgcga gtcatccggc tcatctgcag ggcctcgcga taaaagctgc
     1201 cggtaaaagg gttcaaggcg cgcatgatgt cggcaatcgt gcgatagccg tgcagcttgg
     1261 catggggcga ctcggtccgc tcggcctgat cgagcacgcc ggggttggtc tccttgacca
     1321 tctgctcgca gaaatcgaca ccgaccaggt tgtcctggta gatgttgtgg tcgaacatat
     1381 attcggccgc ttcgccgaga ttgacgatgc actcagcgat attgggggga cggatgccga
     1441 acgccatgtt ctgggcgtag caggcacagg tggcgtgatt gtcgccgcaa atgccgcaga
     1501 tccggctggt gatgaagtga gcgtcgcgcg gatccttgcc cttcatgaaa atgctatagc
     1561 cccggaagat cgacgaggtg ctgtggcact cggcgacgcg ccggttggcg aagtcgatct
     1621 tggtgtagat gccaagactt cccacgatac gcgtgatcgg gtcccacgac atctcgacca
     1681 ggttgcccgc ttgtgcgcga acgtctttct cggtcagggt cacggtcgcc atgtgtctgc
     1741 tccctttctt taagatctcg tcgcgggacg atagccggtc gccagttcgg tgcccctttt
     1801 tcgccacttc ggttccttgt tcgcggtgtc ctgggtgaat ccgcgcaggg cgcggatcac
     1861 gcggccgtag agcccgatcg cggtggaaga cagccgagcg ccaggcggct cgtccatgaa
     1921 cggcatgaac ttgtcgggaa agccgggcat cgtgcagccg atgcagatgc caccgacgtt
     1981 gggacaaccg ccgatgccgg ccatccagcc gcgcttgggc acgttgcaat tgacgaccgg
     2041 tccccagcag ccgagcttga ccaggcattt gggcgaaccg tactcatggg cgaagtcggc
     2101 ctgctcgtag tagccggcgc ggtcgcaccc ttcgtggacg gtcttgccga agagccacgt
     2161 cggcctcagt tggtcgtcga gcggaatcat cggggccagc cccgcgacct gatagagcag
     2221 gtacagtaat gtctccatga aattgtcggg ctgcaccggg cagccaggta cgttgacgat
     2281 cggcaggccg gctttcgatt tccattgcca gcccaggtag tcggccagtc ccatacagcc
     2341 tgtgggattg ccctccatcg cgtgaatgcc tccgtaagtg gcacaggtgc cgatggcgac
     2401 gaccgccagt gccttgggcg ccagccggtc gatccaatcg caggtgggga tcggctgtcc
     2461 ggtctttatg tccgtaccga gcgcggccca gtatccctct ttcttgatct tctcgttagg
     2521 aatcgatccc tcgacgacca ggacgaaggg atcgagcctc ccctcctccg cctggtacca
     2581 gaaccgcatg aactcatcgc ccacttcata ggcaagaact ggattatgta gatgaacctt
     2641 cggtagcccg ggaatggctc ccaagaggac gtcctcgatg gagggctggg tggcggcggt
     2701 gatcgacacc gagtcgccgt cgcagcccaa cccggcggtg atccagagga catgcacctc
     2761 tttgacggag ggcgcccgct gggtcttgcg tccgtaggga acggcggttt cggccattgc
     2821 tagagctccc ttcggttggg cccggtcgcc taaagttggc gccagacaaa cacgattgcg
     2881 aacgtcaaca gattctcggc aactgctcgc ccgaaagcat ggcaaccacc cgctcgccgc
     2941 cgatcagcga tcgcagggtg accattcccg gatgggccga cacaacctca ccgatctctt
     3001 cggcctcgcg tcccagggga tgagcgcgca taactgcgac gagtttttca gcttccccag
     3061 gcgcgacgac agcgatcagc tttccttcat tggcgacata aagcgggtcg agccccagaa
     3121 tctcgcaggc gccacggacg tccgcgcgga ccggaatctt ggactcatcg agcttgacac
     3181 cgacgtgtga tgcggcggcc agctcgttga gggtgctcga aactccgccg cgcgtcgggt
     3241 cgcgcatggc gcgaataccg gggcacgatt ccagcatggt ctcgaccaat ccgctaagtg
     3301 gcgcggtgtc gctctcgacc acagtctcga attcgatccc ctcgcggacc gacatgatcg
     3361 ccaccccgtg atcgccgatc gtccctgaga cgatgatccg gtcaccgggt aatgcagagc
     3421 ggatggacag cgatcgacct tggggaacca ggccgacgcc ggtggtggtg atgaagacgc
     3481 cgtccccttt gccccggtca acgaccttgg tgtcgccggc cacgagcgcg acgttcgctt
     3541 cgtcgcatgc ctggcgcatc gacgccgcga tccgctcgag ctggggaatg gcaaggccct
     3601 cctcgaggat gaaggccgct gtcaagtaga gcggccttgc cccaccgacg gccaggtcgt
     3661 tgaccgtgcc gtgaaccgcc agccggccga tgtcgccgcc gggaaagaag agaggacgaa
     3721 ccacaaacga atcggtggtg atggcgatcc gcggcgcatc ggcccccctg cggccgggca
     3781 ggctgacggt tgcctggtct tccagcgcgg ccagcacgtc gttgccgtag gcgggcaaaa
     3841 acagccgctc gatgagctgg gcactcagga gcccgccgcc gccgtggccg agcacgatgc
     3901 ggtcgtagga cgagattggc agcggacagg tgccgttgaa ctctcccagc cttgccatca
     3961 tcggtctcct accgcgagct cgtgggggag cgacgacggg cccgccggat ggatgagagt
     4021 accggtcgaa tcgagccgcg ccgcctccag atggcggccg taggcgtgat aggcggcaca
     4081 cgccccctcc gacgaaacca tcgtcgcacc cagcggcgtt tgcggagtac actcgcggcc
     4141 gaaggccgga caatcatgag gtttcttcag ccctttcaag atctgaccgc taatgcagac
     4201 cgatgactcg tgcgcctcaa tatggtcaac ctcaaagagc cgctcggcgt cgtggtcgcg
     4261 atactccgca cgtagccggt agccgctttg ctcgatcaag ccaatgcccc gccacttgcg
     4321 atcgcagatc tcaaagacgt cgcccaacag ccgccgcgag gcgggattgc cgtcgcgtcg
     4381 tacggcccgg gcatagggat tctcaacctc ggcgcgaccc gactcgagct ggcgaatcgt
     4441 ccacagaatg ccctggagca agtcgagcgg ctcgaagccg gtgatcacga tcggcacccg
     4501 gaaatcgcgt gcgatcggca cgtaatcgcc acagccgacg acggtgcaaa catggccagg
     4561 accgagaaag ccctggatcc ggttatccgg tgcggccaga agggccttca tcatcggtgg
     4621 caccaatacg tgcgacacca gcatgctaaa attggtcagc ccccgctttc gtgccagggc
     4681 gaccgccatg gcattggggg gcgccgtggt ttcgaagccg atcgcaaaga acacgacgcg
     4741 gcggtgagga ttggctgccg cgagattgac cgcgtcgagc ggcgaataga cgatccggac
     4801 gtcgctgccc cgcgactttt gctggaggag gtcgccgcgc gacccgggca cccgcagcat
     4861 gtcgccgaac gagcagaaga tgacgtcggg ctgggcggcg atcgcgtgcg ccttgtcgat
     4921 cgtttcgagt ggtgtcacgc agaccgggca gccagggccg tgcaccagct ctaccaaggg
     4981 cggtaggatc gcgtcgagcc cgtacttgac gatcgtgtga gtctggccgc cacagacttc
     5041 catgatcgtc cagggccgag tgactgtgcg ggcgatgaca gccgccacct tttccgcagc
     5101 cgcgccgtcg cgatactccg taagatactt catggcgatg gctcctccaa atcgttgagc
     5161 tgatccatcg tccccaggaa ctcgaagacg tgcttggcct cggcctcatc gattcgcgac
     5221 aaggcgaaac cgacgtggac cagtacgtaa tcgtcaatgt tggcctcggg gacatgctcg
     5281 aggcagacgc gcttgaggat cccaccaaag tcgaccttgc ccattcgcac gtcgtgctcg
     5341 cgataggtct ctacaacttt gccgggtatg gcgaggcaca tcgctagctc ctcatggcag
     5401 aaactgccat tacgtcggca catgtcgtgc cacgagtgaa atggggcgcg cccgtcgcgg
     5461 cggcaatgac aagctggccc aagctcaacc cgccgtcacc cggcggtacg ttcttgtgtc
     5521 gataaacgcg gaaaccgtcg cgctcgagat gttcaatcac ctcactcgtg agcagtgcat
     5581 tctgaaaaac gcccccgctc aagatcacta cgtccaggtc ctcttgctgc cgcagccgcg
     5641 cgcaaacctg ggcaacaact tcgaccaggg tggaatgaaa ccgccgggca atctgggctg
     5701 cgtcttgccc tctgccgatc gcggcggcaa tggcggcaac gagcgggcgc gtgtcgatcg
     5761 tgaacggtga gccaagttcg gtaccggctg cgatgtcgaa ttcaaaggag gatacatcat
     5821 gagacgaagc ggtcgcggcc agccattcga gttcgattgc ggcttgacct tcgtacgtca
     5881 cgcgatcgcg caggcctagg agagcagcca cgccgtcgaa tagccgtccg gcgctcgacg
     5941 tcatgggcgt attgatccgc cgatcgatta ctcgtcgaac cgtcgtgagc gacgccgcgg
     6001 gcacgcgccc ggccaggagt ccgtccctct gccctgcatc ggcaaggtag gacgcagcca
     6061 tccgccacgg ttcgcgaatg gccaggtcgc cgcccggcat tggtacgtat cggaggtggg
     6121 cggcccggcg aaagccgccg taccctcccg tcaagaactc acctccccaa atcgcgccat
     6181 cggttcccag gccggtgccg tcgaacgcga cgccaatcac cggctcgtca aggagattgt
     6241 cggccatgca actggccacg tgggcatgat gatgctgcac agcgagccgg gcaatcgacg
     6301 ggttgcgatc gtgggcatag cgcgtggtcg cgtagtccgg gtgcaagtcg tggacgacga
     6361 gttcgggctc gataccaaac aagcgttcgt agtggggcac tgcttggcgg tatgcgcgtt
     6421 ctgcttcgta gtactcgagg tcgccgagat gatgggaaag aaaggcgtgg cgtcctcgtc
     6481 ccagagcaaa cgttgccttc aactgtccac caagtgccag gatcggcatc ggacaagaca
     6541 acgggagttc gaccggtgca ggcgcgtagc cgcgtgaccg ccgcagcggc aactcggtca
     6601 ccgcaacgac gcgtgtcacc gaatcgtcgc agcggagatg gatcggccgg tcgtgagtca
     6661 ggaagaggtc ggcgatgccg gccagccgcg cgaccgcgtc ttcgtcctcg taggcgatcg
     6721 gctcatctga tgaattgccg ctggtcatca ccagcggctt gtcgcctagc tcggacaaga
     6781 tcaggtgatg cagcggcgtg tatggcagca tgacacccag agtcggattg cccggcgcca
     6841 ccctctcggc aattcgagcg aaacggtgac ggtgcaagag cacaatcggc cggcgcggtg
     6901 aaatgaggat acgcagctca gccggggaga tctcgcagag cgaccgggcc gactcgacgt
     6961 ccttaaccat gattgccagc ggcttttgat cgcggtgttt gcggcggcgg agtagggcga
     7021 ctgccgcagc gtcgtcggca aggcacgcga gatgatagcc accaagcccc ttgatcgcga
     7081 cgattttctt gtccatgagc gacgcgatgc ccaatgccag cggctggtcg gtgtcgatcg
     7141 gttgtccacg actatcgagt gcccgcaagc gcggaccaca cacagggcac gcgatcggct
     7201 gggcgtgaaa ccggcggtcg cgcgggtctt catactcggc gcggcatgcc gcacacatgg
     7261 cgaacgccgc catcgtcgtg cgctggcggt catacggggc ttcgcgaatg attgagaaac
     7321 gcggaccgca gtttgtgcaa ttgagaaacg gatagcgata gcgacgatcc ctgtggtcga
     7381 acagctcccg caagcagtcg tcacaagtgg cgaagtcggc ggagacgaag atcgaaccgg
     7441 cgagctcgtt ttcgctggat tcgattcgga aatcgggatc gccgcgaggc ggccgtgacg
     7501 accagcggac ctcctcgatt tgcgcgatcg gtggcgggct tgaggagagt tcgtgcaaga
     7561 aacgatcgag cgaatccgac
//
LOCUS       DATAQL010000236         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_634931, whole genome shotgun sequence.
ACCESSION   DATAQL010000236 DATAQL010000000
VERSION     DATAQL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634931"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VHS97_04045"
     CDS             complement(<1..319)
                     /locus_tag="VHS97_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HEX3447395.1"
                     /translation="MPILLAEPDCYPPDLWDNSASIGAKEEAVWWCLHTKPRQEKAIA
                     RDLRVQGIVYYLPLMLKESRTPQGRKIQSIVPLFTSYMFLNGNVNDRLAALRANRLVS
                     VIEV"
     gene            492..1412
                     /gene="truB"
                     /locus_tag="VHS97_04050"
     CDS             492..1412
                     /gene="truB"
                     /locus_tag="VHS97_04050"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX3447396.1"
                     /translation="MKAHQRPGTGNYAGVTGVLVLNKPSGITSRKLVDQVARLASRSK
                     VGHAGTLDPLATGILIVCIGSATRLVENLQDLPKTYRTLVRLGARSDTLDADGQIEIE
                     QSPRIPSLLDIERALQPLTGEVDQRPPAYSALKIQGRRAYDLARAGRALELAPRRVRI
                     DRIDVLHYDWPSLELEIDCGGGTYVRSIARDVGETLGCGGFVQTLVRTRTGPFTLADA
                     IEPALLSSESIHHYLRPAIDAVAGVPKLSIDAGQVEAVAQGRRLCTQDLGAVPFADGQ
                     VALVDREGILIAMAEFDTARGWIQPRKVLV"
     gene            1567..>2854
                     /gene="hflX"
                     /locus_tag="VHS97_04055"
     CDS             1567..>2854
                     /gene="hflX"
                     /locus_tag="VHS97_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX3447397.1"
                     /translation="MTIHVGPSVEERSYPALIDTNRIERSGRQERAILVGVILPHSEV
                     TTEDPLDELRGLAKTAGLMVVGSIVQKRHQIDIATYIGSGKVDELKNVVLAQEADVVL
                     FDNDLGPAQTRNLEKQLEVKVVDRTEVILDIFATHAQTHEAHLQVELAQLEYAMPRLK
                     RMWTHLSRYKGGIGVRGPGEKQLEEDRRLVAHRIQELKAKLAKIQARKEREVASRGDV
                     PTISLVGYTNAGKSTLMNALTEAGVLVEDKLFATLDTRTRRWRFGGGGHALLSDTVGF
                     IRALPHALVASFKATLEEARQAELLLHVVDASSPEAGSQIQAVKSVLAELGLESHPTL
                     LVLNKSDKVPDRSYLDVLKAHHSGSVAISAACGDGLDRLEAAVRSALSQRDLEAEIET
                     GVSNGRVLAYLAQHAQIHNRTYDADRVRLHCHLPKRT"
BASE COUNT          604 a          841 c          867 g          542 t
ORIGIN      
        1 cgacctcgat cacgctcacc aggcggttgg ctctgagggc tgcgagccga tcgttgacat
       61 tgccgttcag gaacatgtaa ctggtgaaga gggggacaat cgattgaatc tttcgcccct
      121 ggggtgtgcg actttccttg agcatcagag gaaggtaata gacaattccc tgaactcgca
      181 ggtcgcgcgc gatcgccttt tcctggcggg gtttggtgtg cagacaccac cagactgctt
      241 cttccttggc cccgatcgac gcggaattat cccaaaggtc aggtgggtaa cagtcgggct
      301 cggctagcaa gatcggcatt acggagctcg gagacgaacc aacgcgggcg gcaatggccg
      361 caggattttc gatcaatctg tctgcagttt tcagcccaac ttagttaaac ccgagagcgg
      421 cgtcaagcga gacgcacggc tgacggcggg ccgtgatagt gagctggctt taacgagcct
      481 ggagacatgc aatgaaagcc catcaacggc cgggaacagg gaattacgca ggggttactg
      541 gcgtccttgt tcttaataag ccatcgggca ttacgtcccg caagctcgtc gaccaggtcg
      601 cgcggctggc atcgcgttcg aaagtggggc acgcgggcac cctcgatccc ctggcgactg
      661 gtatcctgat cgtctgcatc ggatccgcta ccaggctcgt cgagaatctg caagacttgc
      721 ccaagactta tcgaaccttg gtacgattgg gcgcgcggag tgacacactc gacgcggacg
      781 gacagatcga aatcgagcaa tccccgcgga tcccgtcgtt gctggatatc gagcgggccc
      841 tgcaaccgct caccggggag gtcgatcaaa gaccaccggc gtattcggcc ctgaaaatcc
      901 aggggaggcg agcctacgac ctggcgcgcg cgggccgtgc tctcgaactg gccccgcgcc
      961 gcgtgcggat cgaccggatc gacgtgctcc attacgattg gcccagtctt gagctcgaaa
     1021 tcgattgtgg cggcgggacc tacgtccgat cgatcgcccg cgacgtcggc gagacgctcg
     1081 gttgcggtgg atttgtccag accttggttc ggacccggac cggaccgttc acgctcgcag
     1141 acgcgatcga accagcgcta ctctcgtcgg agtcgattca ccattatctt cggcccgcca
     1201 tagacgcggt tgccggcgtg cccaagctat caattgacgc cggccaggtc gaggccgtcg
     1261 cgcaaggccg acggctgtgc acacaagatc tgggtgcagt gccattcgcc gacggtcagg
     1321 tcgcactggt cgatcgcgag gggattttga ttgctatggc cgaattcgat actgcacggg
     1381 gatggattca gcctcgaaaa gtgctcgtct agagcgcggc ttttcgcgcc ggccgaacct
     1441 cgtctcagaa gtaggtgtca aacgagagga aaacgcggac atggtcaaaa tagtcatccg
     1501 tatactgcaa tcatcgttga cggcggatcg gcaatgactt agaatttgga aacagcgagt
     1561 cgtgaaatga cgattcatgt cggtccgtcc gtggaagaga ggtcatatcc cgccttgatt
     1621 gataccaatc gcatcgagcg ttcggggcgt caggaacgag ccattctggt cggggttatt
     1681 cttccccata gcgaagtgac aaccgaagat ccgctggacg agctccgggg tcttgccaag
     1741 acagccgggc taatggttgt cggcagcatc gtccaaaagc ggcatcaaat cgacatagcc
     1801 acatacatcg gctccggcaa ggtcgatgag ctcaaaaatg tggtgcttgc tcaagaggcc
     1861 gacgttgttc tctttgacaa cgacctgggt cccgcccaga ccagaaacct cgaaaagcag
     1921 ctcgaagtca aggtggttga ccgcaccgaa gtgatactgg atatcttcgc gactcacgct
     1981 cagacgcatg aggcccatct gcaggtggag ctggcgcaac tggaatacgc catgccgcgc
     2041 ttgaagcgga tgtggactca cctgtcgcgc tacaaggggg gcatcggggt ccgtggccct
     2101 ggcgaaaagc agctcgagga agaccgccgc ttggtcgcac accgaattca agagctcaaa
     2161 gccaagcttg cgaagatcca ggcgagaaag gaacgcgagg tcgccagccg gggagacgta
     2221 ccgacaatct cgctggttgg ttacaccaac gcgggtaaga gcacgctgat gaacgccctc
     2281 acggaagccg gcgtcttggt cgaggacaag ctttttgcca cgctcgacac gcgcacgcgg
     2341 cgttggcgtt ttggaggcgg aggccacgcg cttttgtcag acacagtcgg cttcatccgc
     2401 gccttgcccc acgccctggt cgcgtcgttc aaggcgaccc tcgaggaagc gcgtcaggct
     2461 gagctgctac tgcacgtcgt cgatgcctcc agcccggaag ccggaagcca gatccaggcg
     2521 gtcaagagcg tgcttgccga gctgggcctc gaaagccacc ctacgctctt ggtcctgaac
     2581 aagtcggaca aagttccaga ccgatcttat ctcgacgtgc tcaaggctca ccactctgga
     2641 tccgtcgcaa tcagcgccgc ctgcggtgat ggactcgacc gcctcgaggc cgccgtgcgc
     2701 tcagcgctca gccagcgcga tcttgaggcg gaaatcgaaa ccggtgtttc caacggccgc
     2761 gtgctggctt accttgcaca gcacgcccaa attcacaacc gcacatatga cgctgaccgt
     2821 gtgcggctgc actgccatct tcccaagcgc acgg
//
LOCUS       DATAQL010000237         3879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1093190, whole genome shotgun sequence.
ACCESSION   DATAQL010000237 DATAQL010000000
VERSION     DATAQL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093190"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VHS97_04060"
     CDS             <1..231
                     /locus_tag="VHS97_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447398.1"
                     /translation="FYSGKFIKFHIPEWLFWLVMVLIAAASLLIRPGAKKTDGDSGLS
                     ERLDLLDSVDESSEEQLAGDLRTQPEKPRSES"
     gene            complement(307..1104)
                     /locus_tag="VHS97_04065"
     CDS             complement(307..1104)
                     /locus_tag="VHS97_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447399.1"
                     /translation="MRLLSSAAQAHPLATIAAERDLVADPVQHPVPTGDQTSAQAIST
                     ETWDEALVQTQLAELLRSPGATASSPTTSATLSPTAQTATTDTDPVAMASGLSQLNKY
                     LNRTWSRAGIPAQLHDDSTQAVYTALLQNMGRGRFDGLVSDVGNWGVKDTFSRETSDG
                     LAFFRAVDMVKKRAQRERFHQSLDTVDVASDDRKSGSGHSLREALKEAIDQTLNPREA
                     ALIEDTLIGKTPAEIATQWGVAAKTVSNEKTRVLQKLRSALANYEMN"
     gene            complement(1549..2646)
                     /locus_tag="VHS97_04070"
     CDS             complement(1549..2646)
                     /locus_tag="VHS97_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX3447400.1"
                     /translation="MQLSAIGLKDTQLDTPALLIDLDALQQNIDLMAGHLRSRGIAWR
                     PHAKAYKCPAIAHLLRRAGAIGVTVAKVSEAEVMAAGGIDDILIAHLVVGPSKCARLA
                     ALQRQANVKATVDNRDHVAPLGQAAADAGTTIGVLVDVDLGMNRTGVATVEAAAELAR
                     HVAATPGLRFDGLMGYEGHTLMIADPAEKRAAIAAALGKLIRARDVVEAAGQECRIIS
                     AGGSGSYQLTADVAGITEVQAGGGIFACRYYTQACHVAGHHPAVSVLATVVSRPNSDR
                     AILDIGQKSVSQHQALPILRDYPDCRFVGLSAEHATVALAPQTNLQIGEKVHVVPGYS
                     DFTFVLHDRVLGLRSGRVEAVWDLLGRGRLQ"
     gene            complement(2694..>3879)
                     /locus_tag="VHS97_04075"
     CDS             complement(2694..>3879)
                     /locus_tag="VHS97_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447401.1"
                     /translation="AANERVRVAVIGLRSRGTDLSRLFAANPGAEVAAVCDVDDSMMG
                     KPVRAVEKIAGKPPRVEKDFRRLLDDKAIDAVAIATPDHWHAMLTVLACQAGKDVYVE
                     KPASHNVVEGRRMVEAARKYNRVVQLGTQRRSTPYVREAIEHVRSGALGKVAMARAWN
                     HQKRVNIGHGQPGPVPPGIDYAMWQGPAPDRAYHANRLHYNWHWFWNWGTGELGNNGI
                     HGLDVARWGLGAVAPIAVSSGGGKYVFDDDQETPDTQTVTWDFPEAALVYEHRIWSNH
                     GTEGELFGIAFYGEKGTLIIGEKGWRVEEGDKAQGAASPDGQARHVGNFVECVRSRET
                     PNADIEIGHLSTRLCHLGNIALRTGKKLAFDAETESFKDAEANALLAREYSRRFEMPS
                     HV"
BASE COUNT          705 a         1198 c         1156 g          820 t
ORIGIN      
        1 ttttacagtg ggaaattcat caaatttcat atacccgagt ggctcttctg gctcgtcatg
       61 gttctgattg cggcggcgag tttgcttatc agaccgggcg cgaaaaaaac tgatggcgac
      121 agcggcctgt ccgagagact cgatttgctc gactcggtag acgagtcatc cgaagagcaa
      181 ctcgctggcg accttaggac gcagccggag aagccgcgtt cggaatcata agcggttcgc
      241 ggggccggca ttgcaactga gagccgagtg acgcaacaaa agatctgcgt gccgtcagtg
      301 ccgcggctaa ttcatctcgt aattggccag ggccgaccga agcttttgca gcacgcgggt
      361 cttttcgttg ctgacagtct tggctgcgac accccactga gtggcgatct ccgccggcgt
      421 cttgcctatg agtgtgtctt cgatcagggc cgcctcgcgc ggattcaggg tctggtcgat
      481 agcttccttg agcgcctcgc gcaaactgtg accggagcct gatttgcgat catcacttgc
      541 cacgtcgacc gtatcgagcg attggtgaaa tcgctcgcgt tgggctcgct tcttcaccat
      601 atcgacggct cggaagaatg cgagcccgtc ggatgtctcg cgactgaaag tgtctttgac
      661 tccccaattg cccacatcgc tcaccaagcc gtcaaagcgc ccacgaccca tgttctgcaa
      721 gagcgcggtg tataccgctt gcgtgctgtc gtcgtgaagc tgagcgggga tgcccgcccg
      781 ggaccaggtc ctgttcaggt atttgtttaa ttgcgacagc ccggacgcca tcgccacggg
      841 gtcggtgtcg gtcgtggccg tctgagcggt cggactgagc gtcgcactcg ttgttggcga
      901 cgacgcggtg gcgccggggg atcttaagag ctcggccaat tgggtttgaa cgagcgcttc
      961 atcccaagtc tcggttgaga tggcctgcgc cgacgtctgg tcgcccgtgg gcacagggtg
     1021 ctggacggga tcggccacca ggtcgcgctc ggcggctatg gtggccagcg gatgagcttg
     1081 cgccgcgctt gatagtaagc gcatcgcctc aagccgttcc accgccgggc ggtaatcccg
     1141 ccgcgcacgc ggattatttt cgtttccgct ggccaagccc caacccccca actcgttcgc
     1201 ttcgtttgct ccgcagtgcc gccgccgcct ctatcatttt aatggccaac gtgcccccat
     1261 aacggacacg cggcgaacga ttttgatggt cccacggttc gatcgacccc gtgtccatcg
     1321 acggaacctt tagccattct atattttgaa catagatcgc caattacccg ttggcgccag
     1381 ttgcgcgtca acttaatcga aacgaaacag gtgttcgaga gcaaatcacg accactaatg
     1441 agtaaatata ccacccatgc acgctattgt ccagatcttg gcgagtagct ctaacttcta
     1501 cgatcgaagt tattgccaat taagaattag caaccacagg cgaccgtatt attgcaggcg
     1561 accccgacct aacaggtccc aaaccgcctc aacacgcccg ctccgaagtc ccagtactcg
     1621 atcgtgaagc acaaaagtaa aatcgctata acccggaacg acgtgcactt tttctccgat
     1681 ttgcaaattt gtctgcggcg cgagcgcaac ggtggcgtgt tccgctgaaa gaccgacgaa
     1741 gcggcagtct ggatagtctc ggagaattgg caaggcttgg tgttggctca cggacttttg
     1801 accgatgtcg aggatcgctc gatcggaatt cggccggctg acgacggttg cgagaacgga
     1861 cactgcggga tgatgccctg cgacgtgaca ggcctgggtg taatatcgac aggcgaagat
     1921 acctccgccc gcctggacct cggtgatgcc ggcgacgtcg gcggtgagtt ggtaagagcc
     1981 agagccgccg gctgaaatga tcctgcattc ttgtccggcg gcctcgacga cgtcgcgagc
     2041 ccggatcagc ttgccaagtg cggcggcgat ggctgccctc ttttctgcgg ggtcggcaat
     2101 catgagggta tgcccctcat aacccataag tccgtcgaat cgtaaaccgg gcgtggccgc
     2161 aacgtgccgg gctaattcgg ccgcggcttc gaccgtcgcc acgccggttc gattcatccc
     2221 caggtcgaca tccaccaaca ccccgatcgt ggtgccggca tccgcggccg cctggccgag
     2281 cggtgccacg tggtcgcggt tgtcgacggt cgccttgacg ttggcctgac gctgcagcgc
     2341 cgccaggcgc gcgcatttgc tgggaccgac gaccaggtgg gcgatcagga tgtcgtcgat
     2401 tccgccggcc gccatgactt ccgcctcgga gaccttggca acagtcaccc cgatcgcacc
     2461 ggcccgccgc agcaagtgtg caatcgccgg gcacttgtac gccttggcgt gcggacgcca
     2521 cgcgatgcca cgcgatcgga ggtggccggc catcaagtcg atgttctgct gaagcgcatc
     2581 gagatcgatc aaaagagctg gtgtgtccag ttgtgtgtcc ttcaggccaa tcgcgctcaa
     2641 ttgcatggtg gtttgttgct cgtatcgggt cgagtatctc ggcaatcttc acgtcaaacg
     2701 tgcgacggca tctcgaatcg ccggctgtac tcgcgtgcca gaagggcgtt tgcttcggca
     2761 tccttgaacg actcggtctc agcatcgaag gcgagctttt tgccggttcg taacgcgatg
     2821 ttgcccaggt ggcagagtcg cgtgctcaaa tggccgatct cgatgtcggc attgggcgtt
     2881 tcgcgactgc ggacacattc aacaaagttc ccaacgtgcc gtgcttgtcc gtcggggctc
     2941 gccgcgccct gtgccttgtc accttcttcg acccgccagc ctttctcgcc gatgatcagc
     3001 gtccccttct cgccgtaaaa agcgatgccg aagagttccc cctcggtgcc gtggttcgac
     3061 cagatgcgat gctcgtacac gagcgcagcc tccggaaaat cccaggtcac cgtttgcgta
     3121 tcgggcgtct cttggtcgtc gtcgaagacg tacttgccgc cgccggaact aacggcgatg
     3181 ggcgctacgg cgcccagccc ccagcgcgca acgtcgagac catggattcc gttattcccg
     3241 agttcgcccg tcccccagtt ccagaaccag tgccagttgt aatgcaagcg gttggcgtga
     3301 tacgcccggt cgggtgctgg cccttgccac atggcataat cgatgcctgg aggtacagga
     3361 ccgggctggc cgtggccgat gttgactcgt ttttgatggt tccacgcgcg agccattgcg
     3421 accttgccca gggctccgga gcgcacgtgc tcgatcgctt cccgcacgta gggggtactt
     3481 cgccgctggg ttccgagctg cacgacgcgg ttgtacttgc gcgcggcctc gaccatccgc
     3541 ctcccctcga cgacgttgtg gctcgctggc ttctcgacgt aaacgtcttt accggcctgg
     3601 cacgccaaga cggtcaacat cgcgtgccag tggtcgggcg tcgcgattgc gaccgcatcg
     3661 atcgccttgt catcgagcaa tcggcggaaa tctttctcga cgcgcggcgg cttgccggcg
     3721 attttttcga cggcacgcac gggttttccc atcatcgagt catccacatc acagacggcc
     3781 gcaacctcgg cccctggatt ggcggcgaac aaacgcgaca ggtcggtacc gcggctgcgc
     3841 aggccgatca cggcgacacg cacccgctcg ttggccgcg
//
LOCUS       DATAQL010000238         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_24866, whole genome shotgun sequence.
ACCESSION   DATAQL010000238 DATAQL010000000
VERSION     DATAQL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24866"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..1403)
                     /locus_tag="VHS97_04080"
     CDS             complement(429..1403)
                     /locus_tag="VHS97_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3447402.1"
                     /translation="MNRREFVGSSLTAAAAPPAPQRVASEVDGNAGGAVAIERHSDGR
                     PHRDKVLAVISPHSDDFTIFNGGLIAKLIDEGYKAYLIRVTNDDMAGPGSIGHTVLAN
                     EQDKDRLVRLFGFAGSFDLNYPNHNMDNTSKAELKSRFIFLIRLLRVDTVISFSPWAH
                     YEENPDHYVTATCVQAACWMAGRDKDYPEHFAAGLKPHAVSEKYYYGRWDPRSDLVID
                     WTINRVVDISPYVEKKVDGLIENRAQGPAGESGARLRGRLAAQGLKLALLGDDDDSAN
                     RAFIREFVLRFNRALGQRHGLAYAEPYFYIGPEPDIVEEYVRQHAVKR"
     gene            2167..>4037
                     /locus_tag="VHS97_04085"
     CDS             2167..>4037
                     /locus_tag="VHS97_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3447403.1"
                     /translation="MFLTRLKAGAVVLLASCTMASLTTLATERAQAGRDDGRPVAASA
                     AVGSASPGPPHDPAADAAAKAKKPQAAGGDGVVQSKGPILTPDGKLVYVNVYSTDPNA
                     DQKDLFNFANVFVMPRLSRRIKGMGIPRILGNLTFAMRVWLKPDRMRAHNLSSVDIMK
                     AVTPSRIVGREYDLRLMSLVEDRSGIPAEGWNLVIVAAVDRVLHFRIFDSDGKKVVDT
                     DEKRLTEQARPIEDLRKQLVSLRPPHELTESEKYQAVDAVLYDPRGDPGAFLELESEN
                     CRVVDAVTSIVGHTRGSAGRHDPFTGKTSQSKEYVQIYTGRYNKPEQYGQIVLRANDE
                     GEILRLKDVGELELIPQLFDNDSDVDGHPSDALVLKQASGSNAADVIEAIKEELEQTR
                     KESVPLGMNFEVIPLENPGMIYAVIKPPLRSTLEYTSAKCHELGAIAKGIDEITSVAS
                     LAGYQIRTEYRGSDAGTCLIQLKNRSDRKLTSRQIIEKLEEKCRTMSVHLEFFEPPAV
                     SVFVAAGGFSVRVLDRTNSNNDERLGRVPETYMDDLLKRKGVERLFVFLASSYPQYEL
                     VINNDVAMRRGVSIAYAMENLAIVVGGNVQAERKSRRLAEDFSTLFVKNDRGELEPYR
                     "
BASE COUNT          773 a         1241 c         1324 g          699 t
ORIGIN      
        1 ggcagttgtg cggcctatcg agccacgctc gcgtccgcct tcccgcgtcg gtccgacgac
       61 accgacgcat cgagctagcg attgggcgca tcggtcgatc cgaccgacgc gcgcaaggcg
      121 accgtccgac gcttcggtcg atccgaccgg cgcgccgcgg gccaacgctt cggtcctaag
      181 gtgttgtcgg ctacccggtc ggcgaggtaa ggcgactgat cggtttgagg actcggcaaa
      241 caaaaatgtg ctgccatggc cctggcgttc gagaaagccg aggctagccg aggacgcgtc
      301 acggacgacc ctcgtcggac gggtctcgga tacccgaccg ctcatagtga cggcgatgcc
      361 ggacgatctg gcggcacgcc ccccgttcgc aacgagtcga aggggatctc ataccgcgag
      421 agccggcgtc atcgtttgac ggcgtgctgg cggacgtact cctcgacgat gtcgggctcg
      481 ggaccgatgt agaagtaggg ctcggcgtag gcgaggccat gccgctggcc gagggcgcgg
      541 ttgaagcgca ggacgaactc gcggatgaag gcccggttcg cggagtcgtc atcatcgccg
      601 aggagcgcga gcttcagccc ctgcgccgcg agccgaccgc gcaaccgggc tccgctttct
      661 ccggccgggc cttgggcccg gttctcgatg aggccgtcga ccttcttctc aacgtacggg
      721 ctgatgtcaa cgacgcggtt gatcgtccaa tcgatgacca gatccgagcg cggatcccag
      781 cgcccgtaat aatacttctc gctcacggcg tggggcttca ggccggccgc gaagtgctcc
      841 ggatagtcct tgtcgcggcc ggccatccag caggccgcct ggacgcaagt ggctgtgacg
      901 taatggtcgg gattctcctc atagtgggcc cacggcgaga aactgatcac cgtatcgacc
      961 ctgaggaggc gaatgaggaa gatgaaccgg ctcttcaatt cggccttgga cgtgttgtcc
     1021 atgttgtggt tcgggtagtt gagatcgaac gagcccgcga agccgaagag cctgacgagg
     1081 cggtccttgt cctgctcgtt cgcgagcacg gtgtggccga tcgagcccgg gcccgccatg
     1141 tcgtcgttgg tgacgcggat gagataggcc ttgtagccct cgtcgatcaa cttggcgatg
     1201 agcccgccat tgaagatggt gaagtcgtcg gaatggggcg aaataacggc gagcaccttg
     1261 tcgcgatgcg gtcgtccgtc cgagtggcgc tcgatcgcga cggccccacc cgcgttgccg
     1321 tcgacttcgg aggcaacgcg ttggggcgcg ggcggggcgg cggcagcggt cagggaactc
     1381 ccgacaaact cgcgcctgtt catggcggct catcccctca cggaatggga tctcacgatc
     1441 gtcaggacgg taggagcgga gcgacccgtt gctcaggcgg gtcgcgccgt gaatcccatc
     1501 aggctacccg gtggtcagtg ccgtcggtag ggctcagcat ggtctcccaa gcgctgaccc
     1561 ggggctcgtc gagcccgaat gaccgatggc agcgcccgga cccgcaacgt ccgatcacgc
     1621 agatcccccc aggatcgtac gggagcgatg caagctacga cggggccgcg acggccaggg
     1681 cgcagtccgg ctccccctcc tgtctcagcg agcgccgacg aaagcagtcc ctcgtaggtc
     1741 ggcctctgga aggacaacta cgagacgcag cccgaccgcc ctgtaccgcg ttgaattagg
     1801 aacgccttgc ggaggaccag tatcctggac ctgccacaaa gtgacgcagg cagctaccgg
     1861 cgtaaccgga tggggctgtc acgatggtcg gccgtgcgtg cgtcggtccg ccgcgccgaa
     1921 gtcgattgat ccgaagatgc ttcggtcgtt ccgaccgacg cgcgcaaaga ggttcggaag
     1981 ccgaatacac cgtcccgcca agccaaggtg agggtgggat ttctggcatg gacggacgcg
     2041 ctggccggtt cggcgtcaga tatgattggg ggaacgacgg acgatcgagg gtctggacga
     2101 gtccagtcag atggcggcgt ggttccctgg tcttccacct cactcacggg aggagttctc
     2161 cagaccatgt tcctcaccag gttgaaggcc ggcgcggtcg tcctgctggc ctcgtgcacg
     2221 atggcgagcc tgacgaccct tgcaaccgag cgggcacagg ccggtcgaga cgacgggcgt
     2281 ccggtcgccg catcggccgc cgtgggcagt gccagccccg gacccccgca tgacccggcc
     2341 gcggacgccg cggcaaaggc gaagaagcca caggccgccg gaggtgacgg tgtcgtccag
     2401 agcaagggcc ccatactcac gccggacggc aagctggtgt atgtgaacgt ctacagtacg
     2461 gaccccaacg ccgaccagaa ggacctcttc aacttcgcca acgtcttcgt catgccccgc
     2521 ctctcgcggc ggatcaaggg catgggcatc cccaggatcc tcggcaacct caccttcgcc
     2581 atgcgggtct ggctgaaacc cgaccgcatg cgggcccaca acctgtcgtc cgtggacatc
     2641 atgaaggccg tcacgccgtc gcgcatcgtc ggccgggagt atgacctgcg attgatgtcc
     2701 ttggtggagg accgaagtgg catcccggcc gagggctgga atcttgtcat cgtggccgcc
     2761 gtggatcgcg tcctgcactt ccgcatcttc gacagcgatg gcaagaaggt cgtggatacg
     2821 gatgagaaga ggctgacgga acaagcccga ccgatcgaag atctccggaa gcagctcgtg
     2881 agtttgcggc ctccgcatga gctgaccgaa agtgagaagt accaagctgt cgacgctgta
     2941 ttgtacgatc cccgaggaga tcctggcgct ttcctggagt tggaaagcga gaactgccga
     3001 gttgtcgacg ctgtcacatc aatcgtcggt cacacccgcg gctcggccgg gcggcacgat
     3061 ccgtttacgg ggaagacgtc gcagtcgaag gagtacgtgc agatctacac cgggcgttac
     3121 aacaagccgg agcaatatgg acaaatcgtt ttgagagcaa atgacgaagg ggagatcctg
     3181 cggctcaagg acgttggcga gctggagttg attccccagt tattcgataa tgactcggac
     3241 gtcgacggcc atccctcgga tgctctcgtc ctcaagcagg cctccggctc caacgccgcc
     3301 gacgtcatcg aggccatcaa ggaggagctc gagcagacca ggaaggagtc ggtccctctc
     3361 ggcatgaact tcgaggtcat tccgctcgag aacccgggca tgatctatgc ggtcatcaag
     3421 cctcctctgc gctcgaccct cgaatacacg agcgcaaagt gccacgagtt gggggcgatc
     3481 gctaaaggca tcgacgaaat cacctcggtc gcctcgctgg caggttacca gatccggacc
     3541 gagtaccgcg gctcggacgc ggggacctgt ctcatccaat tgaagaaccg gtccgaccgc
     3601 aagctgacct cgcgccagat catcgagaag ctcgaggaaa aatgccggac gatgagtgtc
     3661 catctcgagt ttttcgagcc gcccgcggtt tcggttttcg tcgcggccgg gggtttctcc
     3721 gtacgcgtcc tggacaggac gaactcgaac aacgacgagc ggctcggaag agttcccgag
     3781 acgtacatgg atgacttgtt gaaacgcaag ggcgtggagc gcctctttgt ctttcttgcc
     3841 agcagctacc cgcagtatga gctggtcatc aacaacgacg tcgccatgcg gagaggggtg
     3901 tcgatcgcgt atgcgatgga gaacctagcc atcgtcgtag gcggcaatgt ccaggccgag
     3961 cggaagtccc gaaggctcgc cgaggatttc tcaacgctgt tcgtcaagaa cgaccgtggg
     4021 gaactggaac cataccg
//
LOCUS       DATAQL010000239         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3842277, whole genome shotgun sequence.
ACCESSION   DATAQL010000239 DATAQL010000000
VERSION     DATAQL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3842277"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VHS97_04090"
     CDS             <1..1114
                     /locus_tag="VHS97_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3447404.1"
                     /translation="PEETMPVETEMVKFVKIRSRLLSDFHNRPIDLRAGVILPRDFAR
                     QSQRHYPVRVHIGGYGSRFTNVGTMMNPESGFHRTWMADDTPPMILIHLDGAGPLGDP
                     YQVDSANHGPYGAAVTRELIPFVEERFRGIGQGYARVVDGGSTGGWVSLALQIFYPEF
                     FNGAWAFCPDGVDFRSFQLVNIYEDDNAYINQHGFERPASREVSGEVRCTMRHECQLE
                     NVLGTGDSWTLSGGQWGAWNATYGPRGVGGRPGPLWNPATGAIDPKAAIHWKEYDLRR
                     ILEENWTKLGPKLKGKLHIWVGEADDFFLNNAVHQLDSFLSQARPAYEGSITYGPGQG
                     NCWLGISQAAMIKQMAQRMTATSPTSNQLHHQENNP"
     gene            1111..2472
                     /locus_tag="VHS97_04095"
     CDS             1111..2472
                     /locus_tag="VHS97_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX3447405.1"
                     /translation="MMLRLSFRQTGGLSMRRAAFTAILLAITCFVVTAITTRAQETPR
                     NFDESKVGSYTLPDPLLSTTGEKVLTTAQWIDERRPEILRLFESRVYGKVPKPASPIE
                     PKFELRSEDKQALSGIAIRREVSILFSEKPDGPRMDLLLYLPKRADNATRVPVFLGLN
                     FGGNHTIHSDPGITLSRQWMRDRVKGVVNHRATESSRGSDSSSWPVERILARGYALAT
                     AYYGDIDPDYDDGFKNGIHPLFYRPGQTQPAADEWGSIGAWAWGLSRALDYLETAPEV
                     DPRKVVLMGHSRLGKTALWAGAQDARFAIVISVQSGCGGAALSRRIFGETVQHINTSF
                     PHWFCANFRQYNGHEDALPIDQHELIALIAPRPVLVCSAQEDLWADPKGEFLGALAAD
                     HVYRLLGTDGLAVTEMPVPARDQPVKSTIGYRIRPGKHEVTTDDWDAFIDFADLHLKR
                     SGR"
BASE COUNT          599 a          899 c          851 g          590 t
ORIGIN      
        1 acccgaggaa acaatgccgg tcgagaccga aatggtcaaa ttcgtcaaga tccgctcgcg
       61 gctcctgagc gatttccaca atcggccgat cgacctgcgc gctggcgtga tcctgccgcg
      121 cgacttcgcg cggcagtcgc agcggcatta ccccgtgcgg gtgcacattg ggggttatgg
      181 gtcgcggttc acgaatgtcg ggacgatgat gaatcccgaa tcgggatttc accgcacctg
      241 gatggccgac gacacgcccc caatgatcct gattcacttg gacggcgccg ggccgctcgg
      301 agacccgtac caggtcgact cggccaacca cggcccctac ggcgctgccg ttacgcgcga
      361 gcttattcca ttcgtcgaag agagattccg gggcattggc cagggatatg ctcgcgttgt
      421 cgacggcggc tcgaccggcg gctgggtgtc gctggcgctt cagatctttt atcccgagtt
      481 tttcaacggc gcctgggctt tttgtcccga cggcgtcgat ttccgctcgt ttcagcttgt
      541 gaatatctac gaggacgaca acgcttacat caaccagcac ggcttcgaac gaccggcgtc
      601 gcgcgaggtt tcgggcgagg tccggtgtac catgcggcat gaatgtcagc tcgaaaacgt
      661 ccttggaacg ggcgactcgt ggaccctctc aggcggccag tggggcgcct ggaacgcgac
      721 ctatgggccc agaggcgtgg gcggccgtcc cgggcccctc tggaatcccg ccacgggagc
      781 gatcgacccc aaagccgcca tacactggaa ggaatacgac ctgcggcgaa tcctcgagga
      841 gaattggacg aagctcgggc ccaagctcaa gggaaaactc cacatctggg tcggtgaagc
      901 cgacgacttc ttcctcaata atgccgttca ccagcttgac tcctttctgt cccaagcgcg
      961 acctgcctac gagggatcga ttacttatgg ccctgggcaa ggcaattgct ggctgggaat
     1021 ctcgcaggcg gcgatgataa aacagatggc ccaacgtatg accgcgactt cgccgacttc
     1081 aaaccagctt caccatcaag agaataatcc atgatgctca ggctcagctt ccggcaaacc
     1141 ggcggtcttt cgatgcgtag agccgcattc actgcgattt tgctggccat cacatgcttc
     1201 gtcgtaacgg cgatcacgac ccgcgcgcaa gagactccgc gcaatttcga cgagagcaag
     1261 gtcggatctt acaccttgcc tgacccttta ttgtctacga ccggcgagaa agtccttaca
     1321 acagcccaat ggatcgacga gcgccggccg gaaatcttgc gactctttga atcccgcgtc
     1381 tatggcaaag tgcccaaacc ggccagtccg attgagccca agtttgagct cagatcggaa
     1441 gacaagcagg cgctatctgg aatcgcaatt cgtcgcgaag tatcgatcct tttttcggag
     1501 aaacccgacg gtccgcgcat ggatctctta ctttacctgc ccaagagggc cgacaacgcg
     1561 acgcgcgtac cagtttttct ggggctgaat ttcgggggaa accatacgat tcactccgat
     1621 ccgggaatca cattgtcgcg ccaatggatg cgggaccgtg tcaaaggggt tgtcaaccac
     1681 cgggcaaccg agtcgtcgcg cggttccgac tcgtcgagct ggccggtcga gcgaatcctt
     1741 gcacgcggct acgcgctcgc gacggcatac tacggcgaca tcgacccgga ttacgacgat
     1801 gggttcaaga atgggattca tccgctattc taccgtccgg gtcagacaca gccggcagcg
     1861 gacgaatggg gttcgatcgg tgcgtgggcg tggggtctct cgcgcgcact agactatctc
     1921 gagaccgcgc ctgaggtcga cccacgcaag gttgtcctca tgggccattc ccggctgggc
     1981 aagacggcac tatgggcagg cgcacaagac gcgcggttcg cgattgtgat ctcggttcaa
     2041 tcgggatgcg gcggcgctgc cctctcgcga cggatatttg gcgagacggt ccagcatatc
     2101 aacacgtcgt tcccacattg gttctgcgcc aactttcgac agtacaatgg ccacgaagac
     2161 gcgctgccga tcgaccagca cgagctgatc gccctgatcg cccctcggcc ggtcttggta
     2221 tgttcagcgc aagaggacct gtgggcggac ccgaaagggg agttcctggg ggccctcgcg
     2281 gccgaccacg tctaccgcct gctgggcacc gacggcctgg ccgtcaccga aatgcccgtt
     2341 ccagcccgcg atcaaccggt caaaagtacc atcggttacc gtatccgccc cggcaaacac
     2401 gaggtgacaa ccgatgattg ggacgcgttc atcgacttcg ccgaccttca cttgaaacgg
     2461 tcaggccggt agcgcccgcg ctcggtgcgg atgtgccaat ttggacgcgt cagcacacgc
     2521 aaggcctgct cgctctatct cgtcgattcc gactctggcg cacgggccgc aacacccgca
     2581 ctgcttgaaa tggcctcgtt tggtcgattc tgcgcgctat cgccgcagcg ctataaacat
     2641 tttagaagat gtaagaaaac ttatatctgt gatattgatt aaccggcgct cacttccggt
     2701 tgcgccgcca ttgccagtta acagatgctg ggtgtttctc gcccggactg tgtgcttaac
     2761 tcggcagtcg gttcgccagg tggctgaccg agcaggaaga agttcgaaag cgtctcagtc
     2821 tgttggagga ccagttacga ccgaacctct tgcacgaaaa aaccgcaaac ctagtctgac
     2881 cccgagtcct tatagtctga ccccaagtcc ttattggaag cccggttcgt accccttcg
//
LOCUS       DATAQL010000240         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2620605, whole genome shotgun sequence.
ACCESSION   DATAQL010000240 DATAQL010000000
VERSION     DATAQL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2620605"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VHS97_04100"
     CDS             <1..321
                     /locus_tag="VHS97_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEX3447406.1"
                     /translation="ANLFALRATDPAAMRQADDPVGPGNIDWLQRIVNMTVNQMSRYR
                     GGRVICAWGTHGSYMDQDQNVLGWIEDICDPMCLGVTQDGQPRHPLYVPYSADLVAFN
                     ALVR"
     gene            371..1063
                     /locus_tag="VHS97_04105"
     CDS             371..1063
                     /locus_tag="VHS97_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447407.1"
                     /translation="MNPHRKFHPELQACELEDRLVPVVSNLGTIVLTTGGYVLLNPFP
                     GASSTPGSSSSGTAIPTSFSMTGSGGISSVQPGNSTGIPGLAATATAGATGGTDGSSG
                     GPAGSSGGSGATINVGSGGNNATAAISIPVVNRNTIANDAVNPVPQTVRPSGDGSPVL
                     PAGQVYRGGVPQTVPTPPSSETPGGQPNRSPGQNPVDYSSPNPLGAAPAHPTPDFSGD
                     SIKALTDRDRLP"
     gene            complement(1072..2160)
                     /locus_tag="VHS97_04110"
     CDS             complement(1072..2160)
                     /locus_tag="VHS97_04110"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX3447408.1"
                     /translation="MATITREELRFLATPEKGEVSALLVRPANASHVLVLGHGASTNM
                     RHATLENIAECMAGAGIATFRYNFPYMEHGKGRDSEEVCTSTIRSAVAAAHQATIDLP
                     VLAGGHSFGGRMTSTAASQMPLDRVCGLVFFAFPLHQPGKPDTKRAHHLDAVTLPMLF
                     LSGTRDDLADWDLLRTVCKKLGKRATLHPLETADHGFRVLKRSRQSDEDVFVEMARVA
                     RDWASKIIPFPASDRAAGTSFAWVDKSRISQHEGSETGDMNMPRGDKSKYTEKQKRKA
                     EHIEEGYEKRGVPEKEAASRAWATVNKESGGGNKSGSGRDVPDTHESSEKGGRIGGAA
                     SASRSAAERSASAKKAAATRKRNKAAKA"
     gene            2366..2830
                     /locus_tag="VHS97_04115"
     CDS             2366..2830
                     /locus_tag="VHS97_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3447409.1"
                     /translation="MNPTIRILGLEDAAVLDRVAPGVFDNAVDPRWAAEFLADSRHHL
                     AVALDADLVVGMASGVHYVHPDKPPELWVNEVAVAPTHQGRGIGRQMLEALFERGRSL
                     GCRGAWVATEPSNTKARRLYAAVGGVEASEESLIVEFCLLANEESTSPISRA"
     gene            complement(3198..>3455)
                     /locus_tag="VHS97_04120"
     CDS             complement(3198..>3455)
                     /locus_tag="VHS97_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX3447410.1"
                     /translation="RKRSTQANTVQWMTFLASLPITVDEHTHFHAIGDITALGRSHNL
                     SAYDAAYLELAMRRCLPLATRDEKLKAAARAVGVILYGFFE"
BASE COUNT          634 a         1038 c         1081 g          702 t
ORIGIN      
        1 gccaacctgt tcgccctcag ggccactgat cccgcagcga tgcggcaagc cgacgacccg
       61 gtcggcccgg gcaatatcga ttggctgcag cgaatcgtaa atatgacggt gaaccagatg
      121 agtcgctacc gtggtggccg cgttatctgt gcttggggaa cgcatggaag ctacatggac
      181 caagatcaaa acgtcttggg gtggattgag gatatctgcg atcccatgtg tctgggtgtg
      241 acgcaggacg gccaaccgag gcaccccctc tacgtgcctt atagtgccga cctggttgca
      301 ttcaatgcac tcgtaagata gacacgagga tgaaccacga gcaccgcgtc tttgtcgagg
      361 agatctcaca atgaatccgc atcggaagtt ccatcccgaa ttacaagcct gcgaactgga
      421 agaccgtctc gtgccggtcg tctccaactt gggaacgatc gtgctgacga ccggcggtta
      481 cgttctcttg aatcccttcc cgggcgcctc ttccacgcca ggcagttcct cgagcggcac
      541 cgcgatcccc acgtcgttct ccatgacggg ctccggtggg atatccagtg tgcagcccgg
      601 taacagcacc ggcatccccg ggctcgccgc gacggcgacg gccggcgcga ccgggggcac
      661 ggacggatcg agcgggggcc cggccggatc aagcggaggc agcggtgcga cgatcaacgt
      721 cggctcggga ggcaataatg ccaccgccgc catcagcatt cctgtggtca atcgcaacac
      781 gatcgccaac gacgctgtga atccggttcc ccagaccgtc cgtccgtccg gagatggctc
      841 tcccgtcttg cctgccggtc aggtctatag gggcggcgtc ccgcagaccg tgcctacccc
      901 gccgtcgtcg gaaacgcctg gcggacagcc gaaccggtcc ccgggccaga atcctgtcga
      961 ttattcgtcc ccgaatccac tgggcgcagc gcctgcacac cctacgcccg atttctcggg
     1021 tgattccata aaggccctca cggatcggga tcggctgccg tgatcaaaag gctacgcctt
     1081 cgccgccttg ttccgcttgc gggtggcggc ggctttcttg gctgaggccg accgctctgc
     1141 ggccgagcgc gaggctgagg cggcgccacc gattctgccg cccttctcgc tggactcgtg
     1201 ggtgtcgggc acgtcgcggc cgctgcccga cttattgccg ccgccggact ccttgttaac
     1261 agtcgcccag gcacgcgacg cggcttcctt ctcggggacg ccgcgcttct cgtaaccttc
     1321 ctcgatatgc tcggccttgc gcttttgctt ttcggtgtac tttgatttgt cgccgcgggg
     1381 catgttcatg tctccggttt ccgagccctc gtgttgggaa atccgcgatt tgtctaccca
     1441 tgcaaacgaa gtgccggcag cgcgatccga ggccgggaat ggaatgattt tgctcgccca
     1501 atcgcgggcg acgcgtgcca tctcgacgaa gacatcctcg tcgctctggc gggagcgttt
     1561 gaggacgcgg aagccgtgat cggcggtctc gagcggatga agtgtggcgc gcttgccgag
     1621 cttcttgcag actgttcgga ggagatccca atccgccaga tcgtcgcggg taccgctcag
     1681 gaagagcatg gggagagtaa cagcgtcgag atggtgggct ctcttcgtgt cgggcttgcc
     1741 gggctgatgc aaaggaaagg cgaagaacac gagaccgcac acgcgatcga ggggcatctg
     1801 cgaagccgca gtggaggtca tgcgaccacc gaacgagtgg ccgccggcca agacggggag
     1861 gtcgatcgtg gcctgatggg ccgccgccac cgccgaacgg atcgtcgatg tgcagacctc
     1921 ctccgaatcc cgccctttgc cgtgctccat gtagggaaaa ttgtagcgga acgtcgctat
     1981 gccagcgccg gccatacact ccgcgatgtt ctcgagggtc gcgtggcgca tgttcgtgct
     2041 ggctccgtgg cccagaacga gcacgtggct tgcgttggcg ggccgcacca gcaaggcgga
     2101 gacttctcct ttttcggggg tggccaggaa tcgcagttct tcgcgcgtaa tcgttgccat
     2161 cggtgtcctc cctcgtccaa aggctcaact gacatcgtgg cctcatttga taggcagttc
     2221 aagatggtgt gccgaggatg cggttcgacg cgaaggagga gatcgccgat ccgttctgag
     2281 ttcccaacaa ttctgatccg ctccgatcgg attcggtaga ctcggtaatc ggctttcaaa
     2341 catgtggggt gaggagatga tacccgtgaa tccgaccatt cgtatcctcg gcctcgaaga
     2401 cgctgcggtg ctcgatcgcg tcgcccctgg ggttttcgac aatgcggtcg atccgcgctg
     2461 ggccgcagag tttctcgccg actcgcggca ccatctggca gtcgctctcg atgccgacct
     2521 ggtggtcggc atggcctcgg gagtgcatta cgtccacccg gacaaaccgc cggaactctg
     2581 ggtcaatgag gtcgccgtgg ccccaacgca ccaaggccgg ggcattggcc gccagatgct
     2641 tgaggcgctc ttcgagcggg gccgtagcct tggctgccgt ggagcatggg tcgccaccga
     2701 accgtcaaac actaaagccc ggcgcctgta cgcggctgtc ggcggcgtcg aagcgtccga
     2761 agagtcgttg atcgttgagt tttgcttact tgccaacgaa gaatccacat cgccgatcag
     2821 ccgtgcgtga aagaatgatg cccttgatcg cccagcgatg cccgccttgc gacggcgctg
     2881 tcgtagtaga aatggtcctc gtcatgccgg tccgacagaa atgcatccgt gatccaacgt
     2941 caggatattg tcgttcatca cacttttcgt gaaggcgaat gcgaggggtg gtctgcggcg
     3001 aagtgaatcc ggtcgtgtcg gacgagaact ctaggacgat agtgaatgcg tagatggcat
     3061 ctcgtctgtc gtcctggcgg gcgccgtcaa atgaaaatcg gactctgggg gcgtgcatgg
     3121 aggagactcg ctcatgtccg agttcggatc agtatcgatg ctcgatattg gactcgatcc
     3181 ctttctcaga agccgagtca ctcaaagaac ccgtaaagaa tcacacctac ggccctggcg
     3241 gctgctttca gtttctcgtc ccgcgtcgcc agtggtaggc agcgccgcat ggctaattct
     3301 aaatatgcgg cgtcatacgc cgacaggtta tgcgatcggc caagggcggt gatgtctccg
     3361 atggcatgga agtgcgtatg ctcatcgacg gtgataggca gtgatgcaag aaaggtcatc
     3421 cattgaactg tgttggcctg agtcgaacgt ttccg
//
LOCUS       DATAQL010000241         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2925204, whole genome shotgun sequence.
ACCESSION   DATAQL010000241 DATAQL010000000
VERSION     DATAQL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2925204"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VHS97_04125"
     CDS             complement(<1..240)
                     /locus_tag="VHS97_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447411.1"
                     /translation="MGEERNRLAATVDDEKRGRAVCCTGIRSRRYRGGSTVVGLLSVL
                     AVLGLWPFAPSAKAQDTPAKRDAPGQPEVPQALAPA"
     gene            complement(483..1943)
                     /locus_tag="VHS97_04130"
     CDS             complement(483..1943)
                     /locus_tag="VHS97_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447412.1"
                     /translation="MARDGCWTWTAAATASLILLMGWPNSVARAEARATVSLRESKRN
                     GASTRVQTELKAKGLYRPGLPPGDASVDGKMPKPLQVEIETRFIFNERLVVNLNGTPG
                     SASIDHVDAAADQLGKGGSLRAVRHVVQAASAINGEVRLMSSLIRPEVRLLVAERRDP
                     GGSVVVVSPAGPLTWYELELVQGLGDPLILGDLLPDRPVKVGDRWRVRDAAAQALSEY
                     DSLTSNKLEATLESADAPKARIRLKGQIQGSARGGVGKMSCEGFLTFDLQAALVDHLD
                     LNRVESRQAGPVEAGLDIKSTLTVTRQAAEPPPELSDAALGGIPLEITPERELLRLIT
                     PSGKAAVLHDRHWHMFWEDPKLIVLKRLSGGQVIAQCNLMVGPNAGKARHQDPTQFRD
                     DIRRALKQRFVRYLGAGEIDGNPAGGFRYKVGIQGREGKLGIIWYYFLIASPEGEQLL
                     ATYTLAEDHMQVFGEQDLLMIGSLQWLKRSQPESRD"
BASE COUNT          491 a          789 c          727 g          560 t
ORIGIN      
        1 ggccggcgcc agtgcctgtg gcacctcggg ttggcccggg gcatctcgtt tggccggagt
       61 atcctgagct ttggctgatg gtgcgaatgg ccaaagtccg agcacggcca ggacgctcaa
      121 taatccgaca accgtgctcc cgccccgata ccgtcgtgag cgaatccctg tgcagcagac
      181 cgctcgccct ctcttttcgt cgtccaccgt ggcggccaaa cggtttcgct cttcacccat
      241 ggtgcaccgt cttcggatct tagtttttgt tggcattccc caaccagatc gctcgaccac
      301 gatcgggtgg ccctgacgaa tcgatcccgc gctttctatt agatcataat ataggccaca
      361 cccggtacgg tagagatcgc ggaagacccg cagttccgcg aggtgatctc atgcatccgc
      421 atcgtcgagg cgcagacatc cttatcccgc tccgcgcacg gatcatttct ccgatgagac
      481 gatcaatcgc gcgattccgg ctgcgaccgc ttgagccact ggagcgagcc gatcatcaag
      541 aggtcctgtt cgccgaatac ctgcatgtga tcctcggcca gcgtgtaggt ggccagaagt
      601 tgttctccct ccgggcttgc gatcagaaag taataccaaa tgataccgag tttaccttca
      661 cgtccttgga tacccacctt gtaacggaag ccgccggccg gattgccgtc gatttcgccc
      721 gcgcccaggt atcgcacgaa tctttgtttg agagcgcggc gaatatcgtc gcggaactgg
      781 gtcgggtctt ggtggcgagc tttgccggcg ttgggaccga ccatcagatt gcactgggca
      841 atgacctggc ctccgctgag ccgtttcagg acgatcaact tgggatcttc ccagaacatg
      901 tgccagtgcc gatcgtgcag cacggccgcc ttgccactcg gtgtaatcaa ccggagcagc
      961 tcgcgctcag gagtgatctc gagcgggatt ccacccagtg cagcgtcgga aagctccggc
     1021 ggcggttcgg ccgcttggcg cgtcacagtg agtgtactct tgatgtcgag ccccgcctcg
     1081 accggtccgg cctgccggct ctcaacacga ttcaaatcca gatggtcgac cagggccgcc
     1141 tggagatcga acgtcaaaaa accctcgcaa ctcatcttcc cgaccccgcc tcgggcggag
     1201 ccctggattt gccctttaag acgaatgcgg gccttcgggg catcggccga ttcgagcgtg
     1261 gcctcgagct tgttggacgt gagcgagtca tattcgctca gagcctgtgc cgcggcgtca
     1321 cgaacgcgcc aacggtcccc gacctttacc ggccggtcgg gtaacaggtc gcccagaatc
     1381 aatgggtctc cgagtccctg tacaagctcg agctcatacc acgtcaaggg cccggcggga
     1441 ctgaccacga ccaccgagcc gccagggtcc cgccgctcgg cgaccaagag cctgacctcc
     1501 ggccggatca gagacgacat gagcctcact tcaccgttga tcgccgaggc ggcctgaacg
     1561 acatgccgca ccgccctgag acttccacct ttcccgagtt ggtccgccgc agcatccacg
     1621 tgatcgatgc ttgccgaccc cggcgttccg ttgaggttaa cgacgagtcg ttcgttgaaa
     1681 atgaaacgcg tctcaatttc gacctggagg ggcttgggca tcttgccgtc gacggaggcg
     1741 tctccgggcg gcaagccggg ccgatacaat ccctttgctt tcaactcggt ctggaccctc
     1801 gtgctggcac cgttgcgctt cgattctcga aggctgaccg ttgccctggc ctccgctcgc
     1861 gccacgctgt tgggccaacc catcagcagg atcagactcg ccgtggccgc cgccgtccaa
     1921 gtccagcatc catccctggc cattcgattc tcctcgcgcc tgggctcgcc ggatccactt
     1981 cgaccattcc ggcgctgcac cgcgtccatt attgatactt gcaggaagct gtcaatttgg
     2041 cattgcggcg accaagtttc tgtactatcg tggatggatg ttcgttcgct ctccaaatca
     2101 atgcacgctc cgaaaggcaa tgcgcatccg aaaaagcgga gaaatctttg atctctccgc
     2161 caacgcggcc gacttgaacg gttgtctcga tcctattgtc taatagcggc ttacgcgtta
     2221 agcgcaaacg atatggggcg gctgcagcga attgagcgaa caaaaatcgt gattcaaacg
     2281 tctgtttgaa acgagcgttg gatttttttg tcgcgttgcg cggttcactt tgctacgact
     2341 ttgacgttga tatgaatagt ttcgagtcat cgcagcatcg aggttctggc acgggttttg
     2401 cattgggctg cttcgaaact gtgctgatgg cattgggacg gatgcgtggg aatgtgattc
     2461 gtgctgagta aacagaacct caatcatggg acgcgcgtca ttgtgtgagg aggcctgtcc
     2521 gcgctcgtca caaaatgaaa cgatccagcg gtgcaaataa tacgtat
//
LOCUS       DATAQL010000242         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4757030, whole genome shotgun sequence.
ACCESSION   DATAQL010000242 DATAQL010000000
VERSION     DATAQL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4757030"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VHS97_04135"
     CDS             complement(<1..737)
                     /locus_tag="VHS97_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447413.1"
                     /translation="MQTFEKAQRWHEGLSAGHWMVLAIASAGWVFDVYEGQLFTIFKT
                     QMLTELASATTAAIEWQGNLGLAAFLLGGAAGGLFFGILGDRFGRVRILAYTILVYSL
                     FSALTAFAQSAPQVHVLRFLVALGAGGEWAVAAALVAETFPSHARAAASGIFHASSIL
                     GSALASLTGMVFVGPGAWRWGFVMGLAPALLVLWIRLSMREPERWVAARRAEVSPAAP
                     ALGNLRELFGRQPWRSRALLGLALATVG"
     gene            complement(782..2278)
                     /locus_tag="VHS97_04140"
     CDS             complement(782..2278)
                     /locus_tag="VHS97_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3447414.1"
                     /translation="MGLFSSSYFRRKDVTHLIGEMNEGERLNRRLGPVALTGLGVGAT
                     IGTGLYVQTGFVANEFAGPSLILSFLLAAIGCGFAALCYSELASMVPVAGSAYTYAYA
                     TLGELLAWIIGWDLILEYAIGSSVVAAGWSNYLDAFLHNVFDFQLDPRLRSAPWDYDV
                     QHGTFMLKTVTLASGQTVMAWLNLPAIFITAAVTVVLVIGVKESAGFNAAMVLLNIGV
                     ILTVVGIGAAYIEPKNWRPFLHEDKGWRGVAEGAGRIFFAYIGFDSISTHAEEARRPQ
                     RDLAIGIMGALTICTVLYVAVAAVLTGMVPYRQIDINAPLSAALQGRGLTFAGGLITL
                     GILAGMTSSLLVGNLSQPRILLAMARDGLLPQGIFAAVHPRFKTPWKSTILVGVVVAA
                     AAALAPLGFLADLVSIGTLFAFVVVSGAVWILRITDPEAPRPFRAPWVPLVSTMGILV
                     NGGMMFSLGPENWLRLLIWLVLGLLIYFGYSRHHSALRMEPIAGARSR"
     gene            complement(2497..>3493)
                     /locus_tag="VHS97_04145"
     CDS             complement(2497..>3493)
                     /locus_tag="VHS97_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3447415.1"
                     /translation="LKLILDTLDRLRVSLPPPPHINRELVDHPSFAPREFAFLLDDFH
                     RRLDALAAYCSRIKALPILIVPGSNDGAYEPSRSVLAARTPTAERAEFAREFQSVRAA
                     ESDDAELAISRYRRLLEQHPEFAESHYRLARLLVKTTDWKEAERHFVLARELDSLVLR
                     CPNDFRAAIRTVAQRYHAVLIDGPAVLASQSPHGILDDHLFHDAQHMNLVGYVALAQQ
                     TLELLAQRRSLGWPESMQVLHIDLKDCADHYGLDATKWSQICERSSYFYRTTAFVRFD
                     PSERLDVARRYDEAARELAAGRPLPRQGLLSLDMPIPIPGRPSSPVAESASAPPS"
BASE COUNT          757 a         1090 c         1083 g          563 t
ORIGIN      
        1 ccgacggtgg cgagtgccag ccccaagagc gcccgcgacc gccacggctg gcggccgaag
       61 agctcgcgca gattgcccag agcgggagcc gccggcgaaa cctcggctcg acgggcggcg
      121 acccaccgct caggttcgcg catggagagg cgtatccaga gcaccaaaag tgctggggcc
      181 aatcccatga cgaatcccca ccgccacgcg ccggggccga cgaaaaccat gccggtcagc
      241 gacgcaagtg cgctgcccag aatgctcgac gcgtggaaga tccccgaagc ggcggcacgg
      301 gcgtgggagg gaaaggtctc ggccaccaag gccgcggcga ccgcccattc gccaccggcg
      361 cccagggcca ccagaaaacg caatacgtgc acctgggggg ccgactgcgc gaatgccgtg
      421 agcgctgaga acagggaata cacgagaatc gtgtacgcca aaatgcggac ccggccgaac
      481 cggtcgccca gaataccgaa aaagagccca cccgccgctc ccccgagcag aaaggccgcc
      541 aggccgagat tcccctgcca ctcgatcgcg gcggtggtgg cgctggccaa ctcggtgagc
      601 atttgcgtct tgaaaatcgt gaaaagctgc ccttcgtaaa cgtcgaagac ccagcctgcc
      661 gacgcgatcg ccagtaccat ccagtggcca gcactcaagc cttcatgcca gcgctgggct
      721 ttctcaaagg tttgcatggc gtggcctcga atggagctgg tgctgtgcga cacgaaggct
      781 cttaccggct ccgtgccccc gcgattggct ccatccgcaa ggccgagtga tggcggctgt
      841 aaccgaaata gatcaagagc cccagcacga gccatatcaa cagacgcagc cagttttccg
      901 ggcccaggga aaacatcatc ccgccgttga ccaggatgcc catggtcgaa accaacggta
      961 cccacggcgc acgaaaaggc cgaggcgctt ctgggtcggt gatccgcaaa atccagacgg
     1021 cccccgacac aacgacgaac gcaaacaagg tgccgatgct gaccaagtcg gccaggaagc
     1081 ccaagggtgc cagggcggcc gccgccgcga caaccacgcc gaccaagatg gtcgacttcc
     1141 agggcgtctt gaaccgggga tgaaccgccg cgaagatgcc ctgaggtaaa agtccgtcac
     1201 gggccatcgc caggagaatt cgcggttggc tcaagttacc gaccaagagc gagctggtca
     1261 tgcctgccag aattcccagt gtgatcaagc cgcccgcaaa cgtcagtccg cggccttgca
     1321 aggccgcgga taatggagcg ttgatgtcga tctggcgata agggaccatc cccgtgagca
     1381 cggccgcgac cgcgacatac aagacggtgc aaatcgtgag ggcccccatg atcccgatcg
     1441 ccaggtcgcg ctgcgggcgg cgggcctcct cggcatgggt cgagatcgag tcgaacccga
     1501 tataagcaaa gaatattctg cccgcccctt ccgctacgcc tcgccaccct ttgtcttcat
     1561 gcaagaaggg gcgccagttt ttgggttcga tatacgcagc gccaatgccc acgaccgtga
     1621 ggatcacacc gatattcaag agcaccatcg ccgcattgaa ccccgcgctt tccttgaccc
     1681 cgatcacgag cacgacggtg acggccgcgg tgatgaagat cgccggcagg ttaagccacg
     1741 ccatgaccgt ctgcccgctg gccagcgtca ccgtcttgag catgaacgtg ccgtgttgca
     1801 cgtcgtagtc ccagggggcc gaccgtagcc ggggatcgag ctggaaatca aagacgttgt
     1861 gcaagaacgc gtccaggtag tttgaccagc cggcggcgac gacgctcgag ccgatggcat
     1921 actcgaggat caagtcccag ccgatgatcc acgccaagag ctcgcccaac gtggcgtacg
     1981 cataggtgta ggcacttcct gctacaggta ccatgctggc cagctccgaa taacagagcg
     2041 cggcgaagcc gcagccgatg gcagccagca ggaacgaaag gatgagcgat gggccggcga
     2101 attcattggc gacgaagccg gtctggacgt agaggccggt gccgatcgtt gctccgaccc
     2161 cgagtcccgt aagagcgacc gggccaagcc gccgattgag gcgctcgcct tcgttcattt
     2221 caccgatcaa gtgtgtgacg tctttacgcc gaaaatagct ggacgaaaac aatcccatcc
     2281 gcgggttcct ctcaccgatt gatcaatcct gatcgaccga gtcggatgac ggcgagaccc
     2341 ttctcgccgt gccgaaatca gtcctgggcg tcgttctcgt catctcccgg tgagcccggg
     2401 cgagtcaaca gactacgaca ccaccgatgc taacgcaaag gctcggtcgc gcgttcgcgt
     2461 gaggttcgtg cccgcacaag aagtttggct tcgaaatcat gaaggtggtg cgctcgccga
     2521 ttcggcaacc ggcgacgacg ggcgccctgg tatcggaatc ggcatgtcca ggctgagtaa
     2581 accttgacgc ggcagcggtc ggcccgcggc cagttcgcgc gcggcttcgt cgtagcgtcg
     2641 cgcgacgtcg aggcgttcgg aagggtcaaa ccggacgaat gccgtcgttc gataaaaata
     2701 cgacgaccgt tcgcagatct gtgaccattt cgtcgcgtca agcccgtagt gatcggcaca
     2761 gtctttgagg tcgatgtgga ggacttgcat cgactctggc cagcccaatg aacggcgttg
     2821 tgcgagcagt tccagagttt gctgggccaa ggcgacataa cccacgagat tcatgtgctg
     2881 cgcatcatga aacagatgat catccaggat tccgtgaggg ctttggctcg ccagcaccgc
     2941 tggaccatcg atcaacacgg cgtgatagcg ctgcgccaca gttcgtatcg ccgcgcgaaa
     3001 gtcgttcggg caccgcagaa ccaagctatc gagctcgcgg gccaagacga aatgacgctc
     3061 cgcttccttc cagtcagttg ttttgaccag aagccgggcg aggcggtaat ggctctcggc
     3121 aaactcaggg tgttgttcca gcaaccggcg gtagcgtgaa atagccaact ctgcgtcatc
     3181 ggactcagcc gcgcgcaccg actgaaactc gcgtgcgaac tcggcgcgtt ccgcggttgg
     3241 ggtccttgcg gccaggaccg atcggctggg ctcataagcg ccatcgttgg atcccggcac
     3301 aatcaggatc ggcaatgcct tgattcggct gcaataggcc gcgagggcgt cgagccggcg
     3361 gtgaaagtcg tccaagagaa acgcgaattc gcgcggggca aagctgggat gatcgaccag
     3421 ctcccggttg atgtgcgggg ggggcggcag ggaaactcgt agccgatcga gcgtatccaa
     3481 gatcaatttg agc
//
LOCUS       DATAQL010000243         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2009607, whole genome shotgun sequence.
ACCESSION   DATAQL010000243 DATAQL010000000
VERSION     DATAQL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009607"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="VHS97_04150"
     CDS             complement(<1..1858)
                     /locus_tag="VHS97_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447416.1"
                     /translation="MRLSLIQLGLLGCLSLVTSCLAVGSTGSPMSWSPDGEWLSYLVV
                     TDATHDALRPGWLFASGVDRPDSVGRERPAGGRSPADGPIYRIWTTHRDGRTSVLIEE
                     SRWPLTAPSWSPRGKSIAFGRFVPVSIETTQAVQRGRLEIVIQDGRDSKHVVWSVPDL
                     ELDPVARADLRNLTCCWSPDGLYLAIPRRWPQPAVEIVRTDTMKRVHILDHATQPAWS
                     PDGTKCAFVRPDGQRDILEYVERHGQTFGKSHLVGAAVRIPAHPYWTSDSRSIIAVVM
                     PSAARLPELFRFNLEPGDSTRVMNLTPEPMRRTAKLRGLSIDFDREAERCFFSVDLEG
                     RESEIVWSIPREKETRKRFHPLDPGQRIESIAVSPDGQMVAARIASPVGLMSPVTYDC
                     ESEQSRLLVPDFDSRRDWIGTLAGAAGALLKASLPPAMADGQVAARPTLLPLPGELPP
                     QEPAVIRVNRLARFGATHCMLPTQRLASGDPEPVNPSETEARLFFNYLRGDYLAAATD
                     LEDLEASFDSPHDRLSLLSVRAQILWARGERPEAFGVIAYLLSSGESNRQLVEETPFG
                     LVFSPYISPTQAWAHYLSARAAEALKSNAGPARDPIGDPVDPHQPDGLIGIPD"
BASE COUNT          476 a          667 c          758 g          409 t
ORIGIN      
        1 tatcgggaat accgatcaag ccatcgggtt ggtgcgggtc gacggggtcg ccgattggat
       61 cgcgggctgg ccccgcattg gacttgaggg cctcggcagc gcgtgctgaa aggtaatgag
      121 cccacgcctg ggtgggactt atataggggg agaaaaccag gccgaacggc gtttcctcga
      181 ctagctgccg atttgactct ccgctcgaaa gcagataggc gatgacgccg aacgcttcgg
      241 gtcgctcgcc gcgcgcccag aggatctggg cgcggacaga aaggagactg aggcgatcgt
      301 gaggggagtc gaaacttgcc tcgaggtctt cgaggtcggt ggccgccgcg agataatcac
      361 ctcggagata gttaaaaaag agacgggcct cggtctcgga cggattgaca ggctccggat
      421 cgccgctcgc gagccgctgg gtgggcagca tgcagtgcgt cgcgccaaac cgggcgagac
      481 gattgacgcg gatgacggcc ggctcctgag gtggcaactc gccagggagg ggcaagagcg
      541 tgggtcgggc agccacctgg ccatcggcca tggcaggcgg gagcgaggcc ttgagtaggg
      601 cccccgcggc accagcaagt gtgcctatcc agtctcttct cgagtcgaag tcgggtacga
      661 ggagccggct ctgctcggac tcgcaatcgt aggtgaccgg cgacatcagg ccaaccggcg
      721 aggcgatgcg cgcggccacc atctggccgt cgggcgagac tgcgatcgat tcaattcgct
      781 ggcctggatc cagagggtga aatcgcttgc gcgtttcctt ttcccgcggg atgctccaga
      841 cgatttcgga ctctcgcccc tccaggtcga cggagaaaaa gcaccgctca gcctcgcgat
      901 cgaaatcaat cgacagaccg cgcagcttcg cagtccggcg catcggctcc ggagtcaaat
      961 tcatgacgcg ggtcgaatcg ccaggttcga ggttgaagcg gaaaagctcc ggcaaccgtg
     1021 ccgccgacgg cataacgacc gcgatgatcg agcggctgtc gctcgtccag taagggtggg
     1081 ccggtatccg gaccgcagct ccgacgagat gggatttgcc gaaggtctgg ccgtggcgct
     1141 cgacatactc gaggatatca cgttgaccgt cagggcggac aaaagcgcat ttggtgccat
     1201 cgggcgacca ggccggttgg gttgcatggt cgagaatgtg cacgcgcttc attgtgtcgg
     1261 ttcgcacaat ctcgacggcg ggctggggcc aacgccgagg tatcgcgagg tagagcccat
     1321 cggggctcca gcagcaggtc aaattccgta aatccgcccg cgcgacggga tcgagctcga
     1381 ggtcgggcac cgaccacaca acgtgtttac tatcgcggcc atcctggatc acgatttcca
     1441 atcggcctcg ctgaactgct tgtgtggttt cgatcgatac gggcacgaat cggccgaatg
     1501 cgatcgattt tcccctcggg ctccaggagg gggccgtgag cggccagcgt gattcttcga
     1561 tcaatacgga tgttcgacca tcgcggtgcg tcgtccaaat gcgatagatc ggaccgtcgg
     1621 cagggcttcg cccaccagct ggccgctctc gtccaaccga atcgggacga tcaactccgg
     1681 acgcgaatag ccaacccggc cggagagcgt cgtgagtcgc gtccgtgacg accaagtagc
     1741 tgagccactc gccatccggc gaccaagaca tcggcgagcc cgtcgaaccg accgcgaggc
     1801 acgatgtgac gaggctcaaa caaccgagaa gtccaagctg gatcaacgac aaacgcatgg
     1861 aacagtacca gtgggatcgg cctggggaga cgcgcggggt ccgcgtctgt tcttgaccgt
     1921 caattcactc gaaaatctgt tcttgggacg aaagccgaac gaatcgtcct cacggtagta
     1981 taaacccatg cacccggact ggatagcgga cgttggccat ctccggcgca cgagcgtact
     2041 ttggccgagt gaaattgagc ccgcgcggca tgaatagggg gcacgagggg agacgagggg
     2101 ccgttgcctc aacagattga ggtggcacgc ttcgatcaag tcagagaatt ctcaccttgc
     2161 cctggttgac aggacgcagg tggattgtat gtttgctgca acgcgttatg tcaaaaaacc
     2221 ggctgatgcg acttccttgg cctcttgaac ctcacggcga cgtcctgcta gattactgag
     2281 ccaacagtac gacacccatg aggctgggtc
//
LOCUS       DATAQL010000244         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2620726, whole genome shotgun sequence.
ACCESSION   DATAQL010000244 DATAQL010000000
VERSION     DATAQL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2620726"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..2086)
                     /locus_tag="VHS97_04155"
     CDS             complement(104..2086)
                     /locus_tag="VHS97_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447417.1"
                     /translation="MTGRRARFTLVGFVACLAVAAGAGDSRALGPADNARSRVFRIHV
                     VEEGTGRGVPLVELKTVNQIRYVTDSNGIVAFDEPGLFDRKVFFSITSHGYEVEKDGL
                     GYRGKALDVSHGGSADIVIRRINIARRLYRVTGGGIYRDSVLTGDPVPIREPLLNGKV
                     FGQDSVVNAVYQGKIHWFWGDTNRPDYPLGNFHVPGATSELPAQGGLEPEIGVNLSYF
                     LDDQGFARPIAPLPGEGPTWISGLVVLKDHDGRERMFATYAKIRKMLEVYERGLAEFN
                     AQSMRFEKVVQFPEASKYAGEFPDGHPFLYQDKGIEYVYYASPYPLVRVPANPDQLKK
                     IELSEAYTCLKPGTKPSLQQFDRGPDGLLRYGWKTRTPLLRQEQQTKLIVERRIKPEE
                     ALLNLRDIDSGKVVVAHGGSVYWNAYRRRWVMITVESYGSTSLLWEVWYTEADSPLGP
                     WVFARKIVTHDKYSFYNPKQHPMFDKDSGRIIFFEGTYTTTFSGNHDPTPRYDYNQIM
                     YQLDLSDPRLALPVAVYELTSGPGAAARLITKTALADRESTTPRPIAFFAPDRQGIAT
                     LPVFEQYDAKNGQTLSVAVNGQASDRSDARPLFFLLPADVKDYTAATAPFYEYREEGS
                     GRRVYSVEPPSLVVRSHWTPKLLGRVWRNPARSRLW"
     gene            complement(2109..4181)
                     /locus_tag="VHS97_04160"
     CDS             complement(2109..4181)
                     /locus_tag="VHS97_04160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX3447418.1"
                     /translation="MRKIRLKSPLNARLAEHFRRDLNESQRAAATAPDGYNLILAGPG
                     SGKTRVITYRVAYLIASGIAPESIMLVTYTRRAAREMIRRMESLIGQPASRVWAGTFH
                     HIGNRLLRRTAGLLGYQPNFTILDSEDQLDLIRLAMDDAGLSGTGKLAPKPAAVHHMI
                     SYSANVNRTLAEVITERDPELAPWQGQLEAAAAAYAQRKLAANSMDYDDLLLQWGRLI
                     REFADERALQGRMFRQILIDEMQDTNAVQVELVEAIAAAGAGNLSAVGDDAQSIYRFR
                     GADYDNILKFAERHSGARIFQLETNYRSTPQIVAFTKASIAHNKSGFDKVLVSARPDG
                     TLPLLVATEDAYQEAAFICQQILEASEKGLPLGQMAVLYRNHHDSILLQGELLARGIP
                     YTVRSGLRFFEQAHIKDVLAHLRVVLNPRDEASWRRLLLLLPGVGPAKAAAIFQHLSK
                     SGKPLESLEEAEAMALLPPKSKGFFAGFVSDLRQLRATDPERNPAAAVGAILKGGYPG
                     TVKLLYERPDNRIADIEQFALLAAKYDSLERLIADLLLAGDVYGMDSADEGDPGEVLV
                     LSTIHQAKGLEWSQVFIPRVVEDSFPHRRALDEPGGEDEERRIFYVAVTRAMNELTLT
                     YPLTIARGGRGPTVFTLPSRFLTEIDDSLVERAEIEIETGANLIGPWSSKLPGRGTGD
                     ELGSWFTE"
     gene            4305..4757
                     /locus_tag="VHS97_04165"
     CDS             4305..4757
                     /locus_tag="VHS97_04165"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX3447419.1"
                     /translation="MGGSAKKTANWPPGLFRYHSPNIPITAIRRYARRIAEKFDPDKI
                     ILFGSYAYGQPDEWSDVDLLVVIPTRNQIDQSLRIVLSATAPFPVDLIVRTPKRMLRD
                     WEDGDWFLREVLAKGKVLYEKTDGAVGSQGRGRHTRRNKPGAERAPAQ"
BASE COUNT          945 a         1408 c         1479 g          959 t
ORIGIN      
        1 ggcgtgggcg tgtcatcggg ttggggcggg gacgatttca tcgtatttct tcctgaatcc
       61 ccaaaaagta acgcgcgggg ctgcgaccca ctttcgccac gcatcaccaa agccgcgacc
      121 gcgccgggtt tcgccagacg cgaccgagta gcttgggcgt ccagtgcgag cggacgacca
      181 agctgggtgg ttcaaccgaa tagacgcgcc tcccgctccc ttcctcgcgg tactcgtaaa
      241 aaggggccgt cgccgcagtg tagtccttga cgtccgcagg gagcaggaaa aacagaggac
      301 gagcatcaga tcggtctgag gcttggccat ttaccgcaac gctcaaagtc tgcccattct
      361 tggcgtcgta ttgctcgaaa accggcaaag tcgcgatgcc ctgacgatcg ggcgcgaaga
      421 aggcgatggg ccgcggcgtg gtggactcac ggtcggctag cgcggtcttt gtgatcaggc
      481 gagcggcggc acctgggccg gacgtcagct catacaccgc cactggtaga gccagccgag
      541 ggtcggacaa atcgagttga tacatgatct gattgtagtc gtagcggggc gtcgggtcgt
      601 ggttgccgga aaaggtcgtc gtgtaagttc cttcgaagaa gatgatccgg ccgctatctt
      661 tgtcaaacat cgggtgttgc ttgggattgt agaagctata tttgtcgtgc gtcacgatct
      721 tgcgcgcgaa gacccagggg cccaggggcg agtcggcctc ggtataccaa acttcccaca
      781 ggagagatgt cgatccgtaa gactcgacgg tgatcatcac ccaccgccgt cggtaggcat
      841 tccagtaaac tgatccgcca tgtgccacca cgaccttacc cgagtcgatg tcgcggaggt
      901 tcaagagggc ctcctcgggc ttgatccgcc tctcaacgat caacttggtc tgctgttcct
      961 gtcggaggag tggcgtacgg gtcttccagc cgtagcgcaa gaggccgtcg ggcccgcggt
     1021 cgaattgctg caggctaggc ttggtgcccg gcttgaggca agtatacgcc tcgctgagtt
     1081 cgatcttttt gagctgatcg gggttggccg ggacgcggac caaggggtat gggctggcat
     1141 agtagacgta ctcgatgcct ttatcttgat agagaaacgg gtggccatcg ggaaattcgc
     1201 cggcatactt cgacgcttcg ggaaactgaa ctaccttttc aaagcgcatc gattgtgcat
     1261 tgaattcggc aaggccgcgc tcgtagacct cgagcatctt gcgaatcttg gcatacgtcg
     1321 caaacatgcg ctctcgcccg tcgtggtcct tgagtacaac taatcccgag atccacgtcg
     1381 ggccctcgcc gggtaagggc gcaatgggcc gagcgaagcc ttggtcgtcg agaaagtagc
     1441 tgagattgac gccgatctcg ggctcgagtc ctccctgggc aggcagctcg gacgtcgcgc
     1501 ccggcacatg gaagtttccc aaggggtagt ctgggcgatt tgtgtcgccc cagaaccagt
     1561 gaatctttcc ctggtagacg gcattcacca cgctgtcctg gccgaatacc ttgccgttca
     1621 agagcggctc gcgaatcgga acgggatcgc cggtaaggac gctgtcgcga tagattccgc
     1681 ccccggtaac tcgatacagc cgtcgcgcga tgttgattcg tcggatcacg atgtcggccg
     1741 aaccgccatg gctgacgtcg agcgccttgc ctcggtatcc caagccatcc ttctcgactt
     1801 catagccgtg gctggtgatc gaaaagaaga cctttctgtc gaacagtccc ggctcatcaa
     1861 aagcgacgat gccgttgcta tcggtgacat aacgaatctg gttaaccgtc ttgagctcga
     1921 ccaaggggac gccgcgtccg gtcccttcct ccacgacatg aatccgaaaa acgcgagagc
     1981 gagcgttgtc ggccggtcca agagcgcggc tatcgcccgc tccggcggcc accgccaggc
     2041 aggcgacgaa tccaacaagg gtaaaccgtg cgcggcgacc agtcattttg cgggatcctc
     2101 ctccacgtct attctgtgaa ccaagacccc agttcatcgc cggtcccgcg ccctggcagc
     2161 ttgctcgacc atggaccgat cagattagct cccgtttcga tttcgatctc cgctcgttct
     2221 accaggctgt cgtcaatctc ggtgaggaac cggctgggga gagtgaagac ggttggaccc
     2281 cggcctccac gagcgatcgt caggggatag gtcagcgtca actcgttcat ggcccgggtg
     2341 acggccacgt aaaagatgcg cctttcctcg tcttcgccgc cgggctcgtc gagagcgcgc
     2401 cggtgcggga agctatcctc gacaacacga gggatgaaaa cttgcgacca ctcaagtccc
     2461 ttggcctggt gaatcgtgga gagaaccaaa acttcaccgg gatcgccctc gtcggccgag
     2521 tccattccgt acacgtcgcc ggccaagagc aagtcggcga tcagccgttc caggctatcg
     2581 tacttggcgg ctagcaaggc aaattgttcg atgtcggcga tgcggttgtc gggacgttcg
     2641 tacaagagct tgaccgtccc gggatagcct cccttcaaga tcgctccgac cgcggcggcg
     2701 gggttgcgtt ccggatcggt tgcgcggagc tgcctgagat cgctgacgaa ccccgcaaag
     2761 aaacccttgc tcttgggcgg caagagcgcc atggcctcgg cctcttcgag cgactcaaga
     2821 ggcttgccgc tcttactcaa gtgctggaaa atcgcggccg cctttgccgg accgacgcca
     2881 ggcaacagca acaagagccg tcgccacgag gcctcgtcgc gcgggtttag gacgacccgc
     2941 aaatgggcga gtacgtcctt gatatgggct tgctcgaaaa atcgcaggcc gctacgaacc
     3001 gtgtagggaa tgccgcgtgc caacagctcg ccttgcaaaa ggatgctgtc gtggtggttg
     3061 cgatagagca cagccatttg tcccagcggc aggccctttt cgctggcctc gaggatctgc
     3121 tgacaaatga acgctgcctc ttgatacgcg tcctcggtgg cgacgagtaa tggcaacgtg
     3181 ccatcgggcc gagcggagac caagaccttg tcaaagcctg acttgttgtg ggcaatcgag
     3241 gctttggtga acgcgacgat ctggggcgta gaccgatagt tcgtttcgag ctgaaagatc
     3301 cgcgctccag aatgccgctc ggcgaatttg agaatattgt cgtagtcggc gccacggaac
     3361 cggtaaatcg actgggcgtc gtcacccact gcggaaaggt tgcccgcccc cgccgctgcg
     3421 atcgcctcga cgagctcgac ttgaacggcg ttggtgtctt gcatctcgtc gatcaggatc
     3481 tggcgaaaca ttcgaccctg cagggcacgc tcgtcggcaa actcgcgtat caagcggccc
     3541 cattgaagga gcagatcgtc gtaatccatg gaattggcgg cgagcttgcg ctgggcatag
     3601 gccgcggcgg cggcctcgag ctgcccttgc catggagcga gctctgggtc acgttcggtg
     3661 attacctcag cgagcgtgcg gttgacgttg gcgctatagc taatcatgtg gtgcacggcg
     3721 gccggttttg gcgcaagctt gcccgtaccg gagagcccgg cgtcgtccat ggccaggcgg
     3781 atcaggtcga gctggtcctc actgtcgagg attgtaaagt tcggctgata acccaacagc
     3841 cccgcggtgc ggcgcaacag gcggttgccg atatggtgaa acgtgcctgc ccagacacgg
     3901 ctcgccggct ggccgatcag tgattccatc cgccggatca tctcacgggc ggctcgtctg
     3961 gtgtaggtga ccaacatgat cgattcgggt gctatgcccg aagcgatcag ataggcgacg
     4021 cgataggtga tgacgcgcgt cttacccgat cccggcccgg ccaggatcag attgtagccg
     4081 tcgggcgccg tcgccgccgc ccgctgcgac tcgttcagat cgcgccggaa atgctccgcc
     4141 aatcgggcat tcaggggcga cttgaggcgt atctttcgca tttaacggcc cgagggcgtg
     4201 agtgtccgtt ctccacggcc acaacgttat tatactaggg tggcgcgggt tctgccgata
     4261 acaaggtttc aagcgccgga aaccgattac cggaccaagg caacatgggt ggatcagcga
     4321 agaagacagc gaattggccc ccgggcttat ttcggtatca ctcgccgaat atccccataa
     4381 cggcgattcg ccgctacgcc cgccgaatcg ccgagaagtt cgatccggac aaaatcattc
     4441 tgttcggctc atacgcgtat ggccagcccg atgagtggag cgacgtggac ctcttggtgg
     4501 tgataccgac ccgcaaccaa attgaccagt ccctccggat cgtcttgtcc gccacagccc
     4561 cttttccagt ggatctgatc gtacggactc cgaagcgaat gctgcgcgat tgggaggacg
     4621 gcgactggtt tctccgggag gtattggcca agggcaaggt tctttatgaa aagactgacg
     4681 gcgcagtggg ttcgcaaggc cgaggacgac acacgcggcg caacaagcct ggcgcagagc
     4741 gagcccccgc tcaatgacct catttgcttt cactgccagc agtcggctga g
//
LOCUS       DATAQL010000245         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1094037, whole genome shotgun sequence.
ACCESSION   DATAQL010000245 DATAQL010000000
VERSION     DATAQL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094037"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VHS97_04170"
     CDS             <1..1563
                     /locus_tag="VHS97_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3447420.1"
                     /translation="KAVNLRRGVPLWEVLATAGKSSTVLRCPCTYPPDSVRGQMLSGM
                     GVPDLPGGLGTSTFYTTSEAVTPRESENVVRPQPAGDRVFTTHLIGPRNLKGGGDLQI
                     EVSLKVDLAGRRLTLQSEGTPRELEVRQGQWSDWLRVKFKVGLLQSIRGMVRFYLVRC
                     EPELALYASPINFDPHSPPFAISEPPEYAGEIARRIGLFYTTGMVEDHAGLNNERISE
                     QAYLDQCEIVWREREKMMLDELEAFKQGFFYCLFDTPDRIQHMFWRFTEPDHPANRGL
                     PPSAEFAHVIDDCYRRCDAVVGKALEFSDDETLFVALSDHGFNSFRRGVNVNTWLNDN
                     GFLALREGVRPGEAAGDLLRQVDWSRTKAYALGLGGIYLNLRGREEQGIVTPEEVEPL
                     KAALARGLTGLPDPEHSNSPTIRRVHPREAIYHGPYLEEAPDLMVDFAPGYRISWSSS
                     MGGIAEILFEDNVKKWSGDHIIDPEHVPGVLFMNRPFRPQGARLLDLAPTILAALGVP
                     KGPAMEGESLLP"
     gene            1560..>2620
                     /locus_tag="VHS97_04175"
     CDS             1560..>2620
                     /locus_tag="VHS97_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3447421.1"
                     /translation="MKILVLGLDCAAPELLFGFEDLPNIRRLMEVGAYGRLESVIPPI
                     TVPAWMCMTTSQDPGSLGIYGFRNRIDHTYVGLGIASARSITEPAIWDHLAREGKRSI
                     IVGVPPGYPPRRINGISVSCFLTPDTDKNVFTHPPELSQEIRQLVGHYPVDVQGFRSE
                     NKAWLRDEIFNMSRTQFQVVRHLLETKPWDYFHFVDIGLDRIHHGFWKYHDPEHVLHE
                     PDSPFRETVHEYYRHIDQEIGRVLELLSDETIVLVVSDHGAQRLDGGFCVNEWLVQEG
                     HLVLKSYPAEVTPFSKLDVDWEKTKVWSEGGYYARVFFNVKGREPQGLIEPALYEAFR
                     DEIKAKFESTTDKDGKPLGT"
BASE COUNT          539 a          748 c          793 g          540 t
ORIGIN      
        1 aaggctgtca acctgcgacg aggggtgccg ctctgggaag tgctggcaac cgccggtaag
       61 agctcgaccg tgttgcgttg tccgtgcacc tacccgcctg attccgtgcg gggccagatg
      121 ctctcgggga tgggcgtccc cgacctgccc ggcggcctgg gaacttccac gttttacacc
      181 accagcgagg ccgtcacgcc ccgcgaaagt gagaacgtgg ttcggccaca gcctgcgggc
      241 gaccgcgttt tcacaaccca tctgatcggt ccgcgcaatc tcaaaggcgg tggcgacctg
      301 cagattgaag tcagcttgaa ggtcgatcta gcaggccgga gattaacact tcagtccgaa
      361 ggcacgccgc gtgaacttga agtgcggcag gggcaatgga gcgactggtt acgggtcaag
      421 ttcaaagtcg ggctgctgca atcgatccgg gggatggtca ggttttatct cgttcgctgt
      481 gagcccgagc tcgccttgta tgcctcgccg atcaatttcg accctcactc gccccccttc
      541 gcgatcagcg aaccccctga atacgccggc gaaattgccc ggcgtattgg cctcttttac
      601 acgacgggca tggtggaaga ccacgccggg ctgaacaacg agcggatcag cgagcaagcg
      661 tatctcgacc agtgcgagat cgtctggcgc gagcgcgaaa agatgatgct cgacgagcta
      721 gaggcattca agcaggggtt tttctactgc ttgttcgaca cgcctgatcg gatccagcac
      781 atgttctggc ggtttaccga gcccgatcac ccagccaacc ggggtttgcc cccgagcgcc
      841 gaatttgccc acgtcatcga tgactgttat cgccgctgtg acgctgtggt tggcaaggcc
      901 ctcgagttca gcgacgacga gactttattc gtcgcgctct cggaccacgg attcaacagt
      961 tttcgccggg gcgttaacgt aaatacttgg ctaaacgaca atggctttct cgcgctgcgt
     1021 gagggagtgc ggccgggcga agccgcgggc gacctgctcc ggcaagtgga ctggtcgcgc
     1081 accaaagcct acgcgctcgg gctggggggg atatatttga atttgagagg tcgagaagag
     1141 caggggatcg tcacgcccga agaggtcgaa ccgctcaagg ccgcactggc gcggggattg
     1201 acgggcttgc cggaccccga gcacagcaat agtccgacca tacgtcgggt gcacccacgc
     1261 gaagcaatct atcatggccc ctacctggag gaagcccccg acctcatggt cgactttgca
     1321 cctggctacc ggatctcatg gtcgtcgtcg atgggaggaa tcgctgagat tttgtttgaa
     1381 gacaatgtca aaaaatggag tggcgatcat atcatcgatc ctgagcatgt gcccggggtg
     1441 cttttcatga atcggccgtt ccgtcctcaa ggggcgcggc tattggatct ggcgccgaca
     1501 atcctggcgg ctctgggcgt tcccaagggc ccggcgatgg aaggagagtc gctcttgcca
     1561 tgaagatcct cgtgctcggg cttgattgtg ccgctccgga actgctcttc ggctttgaag
     1621 acttgcccaa catccgccgg ctcatggagg tgggtgcata tggaaggctc gagagcgtca
     1681 ttccaccgat caccgttccg gcctggatgt gcatgacgac gagccaggac cctgggtcgc
     1741 tgggcattta tggctttcgc aatcgaatcg accataccta tgtcggatta ggcattgcgt
     1801 cggcgcgatc gatcaccgag ccggccatct gggaccatct tgcccgcgaa gggaagcggt
     1861 cgatcatcgt gggtgtccct cccggctatc ccccacgccg aatcaacggc atttccgtga
     1921 gctgtttctt gacaccggat accgacaaga atgtattcac tcacccgcct gagctgtcac
     1981 aagagatccg ccagttggtg ggccattatc cggtcgacgt ccagggattt cgaagcgaga
     2041 acaaggcctg gctgcgcgat gaaatcttca acatgagccg aacgcaattt caggtcgtgc
     2101 gccacctgct cgaaaccaag ccgtgggatt attttcattt tgtcgacatc gggctggatc
     2161 ggatccacca tgggttttgg aaataccatg accccgagca cgtgctgcac gaacctgaca
     2221 gcccattccg cgagacggtt catgagtact atcgccacat cgatcaagaa atcggccggg
     2281 tgctggagtt attgagcgac gagacaatcg ttctcgtcgt gtccgaccat ggggcgcagc
     2341 ggctggatgg cggtttttgc gtcaacgagt ggctggtcca agaaggacac ctggttttaa
     2401 agagttatcc agcggaggtc actccctttt ccaagctcga cgtcgactgg gaaaagacca
     2461 aggtgtggag cgaaggaggc tattacgctc gcgtattctt caacgtcaag ggacgcgagc
     2521 cccaggggtt gattgagccg gctctttacg aggctttccg cgatgagatc aaagccaagt
     2581 tcgagtcgac aaccgacaaa gacggcaaac cgctcggaac
//
LOCUS       DATAQL010000246         5762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5215963, whole genome shotgun sequence.
ACCESSION   DATAQL010000246 DATAQL010000000
VERSION     DATAQL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5215963"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..2927)
                     /locus_tag="VHS97_04180"
     CDS             complement(102..2927)
                     /locus_tag="VHS97_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX3447422.1"
                     /translation="MSHLRSRLVLLSATGCFLLVAILVAARGQQMQPPDPERRLSFGD
                     TDLDGRLSLDEFRELIRNPPRLNKAAAKKANFPIEPVFRRLDADGDGFLTVQEFRRIG
                     QLQPGGPGGPALGPLAKGGFGPLAKAALAKKKTAPGKSPVAAPLKTEAARPAAGKPVT
                     PEQAKFFETKIRPVLMTTCAKCHGNGADKVKGGLYVASPEGLLKGGDTGAAVVPGNLD
                     ESLLITAIRYNDDSLQMPPKTKLPDEVIADFEKWVKMGAPDPRGSRAAAVRSVDLEKG
                     RQFWAFQPPRAVSPPQVKDASWPRSDIDRFLLAALEAKGIKPVADADRHTLLRRASFD
                     LTGLPPSPGEVTAFVADESAAAFARVVDRLLESPRFGERWGRHWLDVARFAESSGKAN
                     LLYSQAWRYRDWVIASFNADKPFNQFVAQQVAGDLLPAQSDCERAESIIATGFLAIGS
                     KIHNTQNRQQFLLDMADEQIDVTGQAFLGLTIACARCHDHKFDPIPQRDYYALAGIFR
                     STQTCYGTLPGLVQNANPAPLIELAAGANQPAAVPKLSPERLAGLQKQLDELIKTRDN
                     LTPDENFTPKGFQTRARIAMLRFRLASYRSDGTPRTYAMGVRERFEPIDSPLYVRGEL
                     DQPGEPVGRGLVQVICQETPPTISGGSGRRELAQWLASPDNPLTARVMVNRIWLHLFG
                     RGLVPTPDNFGAAGQPPYHPELLDALAVAFMRQGWSVKQMIRAIVLSRAYQLGSSHDD
                     HNFEVDPDDTLVWRMSKKRLEAEAIRDAILSTSGRLILEPPVGSAVAEAGEGFAGPFR
                     GFNQDGSDLHRAVYLPVVRDQLPESLTLFDFADPSLATAHRATTSGPSQALYLMNNSF
                     VIRQAEAAADRLRAAAGSEDSRIEAAYLRFLARSPTEPEKIRARDFLAAFNEGRAAPK
                     TTAEREKAAWTAFCQALYASAEFRYLD"
     gene            complement(3022..4011)
                     /locus_tag="VHS97_04185"
     CDS             complement(3022..4011)
                     /locus_tag="VHS97_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447423.1"
                     /translation="MSKTEPTSSAEIDRLVRGNLERNAERVDPRPLFERIQGTLADGS
                     SVRVAAGGLSRKGWPVWKWAGTAAAAAAVVAGLVLFSQDRLALARGETVVREARQAHA
                     LPVDRCYLVEVRRESSLLPELAPTAPQARLTRLWTRGDRFWVESARPQERWAWGRDEA
                     NRFWFAFGRRTAVRLEADEVPYWLNIYCDLHSLNLEKWLAEVLNRFELTRQSPANSPG
                     SSTIRVHARARVTPRQNPGVETADLEIDAETRVVRRMVVRRVWNGQPFATVTYTLAET
                     DELDPNEYLLEGHLEDRSETFTRDHQPERRKELLARWLAPRSPRWAQTIEQMK"
     gene            complement(4004..4555)
                     /locus_tag="VHS97_04190"
     CDS             complement(4004..4555)
                     /locus_tag="VHS97_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447424.1"
                     /translation="MDFPADLRRAREAWVLATLPRALAFANSLLHDRAISEDVVHDCY
                     VRLLEKADTYDLPADGTKLLYKAITNACIDRNYRDRRVLSLEVALSAEPDGPSAVADT
                     RTCDPHELASHQELEQAVACELERLTVAQRGALELQSLGYSLQEIAEALGVSPSNAGV
                     LVHRARKALAERLARFLEEPRDE"
     gene            complement(4960..5331)
                     /locus_tag="VHS97_04195"
     CDS             complement(4960..5331)
                     /locus_tag="VHS97_04195"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX3447425.1"
                     /translation="MTHLPDERRASTRCDVVKNRSSIQFEVPDGRRRIGATLINISRN
                     GALVLAEKPMLRAAPLSIRIDSPVRTDWVDAKVVRFNSNREIGLHFPQGCPDDLLLAA
                     TVGIDLAFLVRNEPNGTTAFD"
     gene            complement(5487..>5762)
                     /locus_tag="VHS97_04200"
     CDS             complement(5487..>5762)
                     /locus_tag="VHS97_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX3447426.1"
                     /translation="QRFSALRRSIPDVTQKMLTDRLKELERDGIVHREVYAVVPPKVE
                     YSLTPYGEGLKPILAAMAKWGTGHKLRKPSASRFRVAGSGQGAEEVS"
BASE COUNT          990 a         1792 c         1927 g         1053 t
ORIGIN      
        1 cgccacgtag ccgaagcccg aggaaacggc cttgaggata tcacggcgcg agacgaacgg
       61 attggggtcg aacatcagcg gcagtcctgt aaaagcggtc gttagtcgag ataacgaaac
      121 tcggcgctgg cgtacagggc ctggcaaaag gcggtccagg cggctttctc acgttcggca
      181 gtcgtcttgg gggcggctct gccttcgttg aacgccgcca agaagtcgcg tgcgcggatt
      241 ttctcgggtt cggtcggcga ccgcgccagg aaccggaggt acgcggcctc aatccgtgag
      301 tcttcagatc cagcagccgc gcgcaggcgg tcggcggcgg cctcggcctg acggatcacg
      361 aacgaattgt tcatgagata aagcgcctgc gacgggccgc tcgtcgtcgc gcgatgggcg
      421 gtggccaggc tcgggtcggc gaaatcgaag agagtgagcg attcggggag ctggtcgcgg
      481 acgaccggca gatagacggc ccgatgcagg tcggagccat cctggttgaa gccgcggaac
      541 gggccggcga aaccctcgcc cgcctcggcc acagccgagc cgaccggcgg ctcgaggatg
      601 agcctgccgc tggttgacag gattgcgtcg cgaatcgcct cggcctcgag ccgctttttg
      661 ctcatccgcc atacgagcgt atcgtcaggg tcgacctcga agttgtggtc gtcgtggctc
      721 gagccaagct ggtaggcccg actcaagacg atcgcgcgga tcatctgctt gactgaccag
      781 ccctggcgca tgaacgcgac cgccagcgca tcgagcagct cggggtggta gggcggctgg
      841 ccggcggcgc cgaaattgtc tggggtcggg accaagcccc gaccgaagag atgcagccag
      901 attcggttga ccatcacccg ggcggtgagc ggattgtccg gcgaggccag ccactgtgcc
      961 agctcgcggc ggccgctccc accggagatc gtcgggggcg tttcctggca gatcacctgg
     1021 acgagcccgc gccctaccgg ctcgcccggc tggtcgagct cgccgcggac gtagagaggg
     1081 ctgtcgatcg gctcgaatcg ttcgcgaacg cccatcgcgt aggtgcgcgg cgtgccgtcg
     1141 gaccggtacg acgccagccg gaaacggagc atggcgatgc gggctcgcgt ctggaagccc
     1201 ttgggggtga agttttcgtc gggtgtcagg ttgtcgcggg tcttgatcag ttcgtcgagc
     1261 tgtttttgga gcccggccag ccgctcggga gagagcttgg gtacggccgc cggttgattg
     1321 gcgccggcgg cgagctcaat taatggagcc gggttggcgt tctgtacaag gcccggcagc
     1381 gtgccgtaac atgtctgcgt gctccggaag atgccagcga gcgcgtagta gtcgcgctgg
     1441 ggaattgggt cgaacttgtg gtcgtggcag cgggcgcacg cgatcgtcag gccaaggaag
     1501 gcctggcccg tgacgtcgat ctgctcatcg gccatgtcga gcaagaattg ctgccggttc
     1561 tgagtattgt ggatcttgct gccgatggcg aggaagccag tcgcaatgat cgattcggcc
     1621 cgttcgcagt cgctctgggc ggggaggagg tcgccggcca cttgctgcgc gacgaactgg
     1681 ttaaatggct tgtcggcgtt gaacgacgcg atgacccagt cgcggtagcg ccaggcctgg
     1741 gaataaagca ggtttgcctt gccgctcgac tcggcgaacc gcgcgacgtc gagccagtgc
     1801 cgcccccagc gctcgccgaa ccggggcgac tcgagcaggc ggtcgacgac cctcgcaaac
     1861 gcggcggcag actcgtcggc gacgaacgcc gtcacttccc cgggcgaagg gggcaggccg
     1921 gtaaggtcga agctggcgcg gcgcaacagc gtatggcggt cggcgtcggc gacgggctta
     1981 atgcccttgg cctcgagcgc ggccagcagg aagcgatcga tgtcggacct gggccacgaa
     2041 gcgtctttga cctggggcgg gctaacagcc cgaggtggct gaaacgccca gaattgccgt
     2101 cctttttcca ggtcgacgga gcgcacggcg gctgcccgcg agccgcgcgg atcgggcgcc
     2161 cccatcttga cccatttctc aaaatcggcg atgacttcgt cgggaagctt ggtcttgggg
     2221 ggcatctgga gcgaatcgtc gttgtagcgg atagccgtga tcagcaggct ttcgtccagg
     2281 tttccgggca cgaccgccgc cccggtatca ccacccttca aaaggccctc ggggctagcg
     2341 acgtacagcc ctcctttgac cttgtcggcg ccgttgccgt ggcacttggc gcaggtcgtc
     2401 atcagcacgg ggcggatctt ggtctcgaag aacttggctt gctcgggcgt gaccggcttc
     2461 ccggcggccg gccgcgccgc ctcggtcttt agaggtgccg caaccggcga cttgccggga
     2521 gccgttttct tcttggccaa ggcggccttg gccagtgggc cgaagccccc ctttgctaac
     2581 gggccgagcg cgggcccccc aggcccgcca ggctggagct ggccgatccg gcggaattcc
     2641 tgtaccgtca gaaacccgtc gccgtcggcg tcgagccggc ggaagaccgg ctcgattggg
     2701 aagttcgcct tcttcgccgc cgctttattg agccggggcg ggttgcggat cagctcgcgg
     2761 aactcgtcca acgagagcct gccatccagg tcggtgtcgc cgaacgacag cctccgttcc
     2821 gggtccggtg gctgcatctg ctggccgcgg gcggccacca ggatcgccac gagcagaaag
     2881 caacccgtgg cggacaatag aaccagtcgg cttcggaggt gcgacatgcg aggttccttt
     2941 gcgaggcggc acgaaggttg atcgagggaa agcacggggc gacgacgtgg cgcacgtcga
     3001 cgccatccag ggcggcaatt gtcacttcat ctgttcgatt gtttgtgccc agcgcggcga
     3061 ccgcggagca agccagcggg ccaaaagctc cttacggcgc tccggctggt gatcgcgggt
     3121 aaaagtctcg gaacggtcct cgaggtggcc ttccaagagg tattcgttgg ggtcgagctc
     3181 gtcggtctcg gccagggtgt aggtgacggt ggcaaagggc tgtccgttcc agacccggcg
     3241 cacaaccatc ctgcgcacca cccgcgtctc ggcgtcgatt tccaggtcgg cggtctcgac
     3301 accgggattc tgccggggag tgacgcgggc cctggcgtgc acccggatcg tcgatgaacc
     3361 cgggctgttc gcagggctct gccgagtcag ctcgaaccgg ttcagcactt cggcaagcca
     3421 cttttccaga ttgagcgagt gcaggtcgca gtagatgttg agccagtacg ggacctcgtc
     3481 agcctcgagc cggacagcag tgcggcggcc aaaggcgaac cagaaccggt tggcttcgtc
     3541 gcggccccag gcccatcgct cctgggggcg agccgattcg acccagaacc ggtctccccg
     3601 ggtccaaagt cgcgtcaagc gtgcttgcgg cgcggtcggt gccagctcgg gcagcagcga
     3661 agactcgcgc cgaacctcga ccagatagca gcggtcgacc ggcagcgcgt gggcctgccg
     3721 cgcctcgcga acaaccgttt ctcccctggc gagcgccaaa cggtcttgag agaagaggac
     3781 aagccctgcg acgacggccg cggcggcggc cgcggtcccc gcccatttcc agaccggcca
     3841 acccttcctc gacagccccc ctgccgcgac ccgcaccgat gagccatcgg ccagggttcc
     3901 ctgaattctc tcgaacaggg gccgcgggtc gacccgttcg gcgttgcgct cgaggttgcc
     3961 ccggacgagc cggtcgattt ccgcgctcga agtcggttcg gttttactca tcgcgaggct
     4021 cctccaagaa acgcgcgagt cgctcggcca gcgccttgcg cgcccgatga acgagcactc
     4081 cggcattgct cgggctgacg cccaaggcct cggcaatctc ttgcagggaa tagcccaggc
     4141 tttgtaactc gagggccccc cgttgggcga cggtcaagcg ctcgagctcg caggcgacgg
     4201 cttgttcgag ctcttggtgc gacgccagct cgtgcgggtc gcacgtccgc gtgtcggcca
     4261 cggcggatgg cccgtcgggt tccgcggaca gtgcgacctc caggcttagc acacgacggt
     4321 cgcgataatt ccggtcgata catgcgttgg tgatcgcctt gtacaggagt ttcgtcccgt
     4381 cagctggcag gtcgtaggtg tctgcctttt cgagcaagcg aacataacaa tcgtggacca
     4441 cgtcttcact gatagcccgg tcatgcaaga gcgagttcgc gaaagccagc gcgcgcggca
     4501 gagtggccag cacccaggcc tctcgcgctc ggcgcagatc cgctggaaaa tccactccag
     4561 acaatgtcgc ctcagggcgg ttcgaagccg attgcgggcc ggccctgccg gttcaggctt
     4621 cggtcctcta atgaagacaa cgggaacggg ttcgatttct tacacgaaaa ctgtgaagcg
     4681 ccatcttatc gcaacggcgc gcccgatcta ggacgacggc gttcgcctgg ccattaaacc
     4741 ccgctgtggg cttggtggag cgatcagcct cgtcttaagc tggcagtatg aggcgatcgg
     4801 aatgaactgc ggcgacgtgt tcaagccgac gatgtggtgc gccaacagtt tcgggcatca
     4861 ttgtctgccg tttcagggcg atgcgcatgg ttttcgcctt gaaaacccag ggcattgccc
     4921 agggggtcct gcaggtgaga tcgtcagccc gacggccggt caatcgaatg ctgtagtgcc
     4981 attgggctca ttgcggacca agaaggcgag atcgatgccc actgtggccg ccaaaagcag
     5041 gtcatcagga catccctgtg ggaaatggag gccgatctct cggttcgaat tgaaccggac
     5101 gacttttgca tctacccagt cggttcgcac tgggctatca atccgtatcg acagcggcgc
     5161 agcgcgaagc atgggcttct ccgcgagcac tagcgcgcca tttcggctga tgttaatcaa
     5221 tgtcgcccct atccttcgcc ggccgtccgg cacctcgaac tggatgctcg accgattttt
     5281 caccacgtcg cagcgagtgg aggccctacg ttcgtcggga agatgtgtca tcggtcatcc
     5341 tcggtctggg aaatgctggc gactgaagtc aaacattcac actttaatct agaacgcggc
     5401 ggccaattgg agaactcgct cgcggcgatt tctctgcgcc gcgtctgcct cgcagtttct
     5461 gtattgggaa cgaaaccggg acacagctag cttacttcct ccgccccttg ccctgaacca
     5521 gcgacccgga atctggaagc agagggcttt ctcaatttat gccctgtacc ccacttcgcc
     5581 atcgctgcga gtatcggctt taagccttcg ccgtacgggg tcagcgagta ctcgactttg
     5641 ggcggtacga cggcgtatac ctcccggtgg acgatcccgt ctcgctccaa ctccttgagg
     5701 cggtccgtca acatcttctg tgtgacgtca gggatggacc gccgcagtgc actgaaccgc
     5761 tg
//
LOCUS       DATAQL010000247         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4757389, whole genome shotgun sequence.
ACCESSION   DATAQL010000247 DATAQL010000000
VERSION     DATAQL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4757389"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1597
                     /locus_tag="VHS97_04205"
     CDS             410..1597
                     /locus_tag="VHS97_04205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3447427.1"
                     /translation="MSGSADLSAGSHRSPEESVTEPMPSHPTFGLIRHWTRTVKRWMR
                     ERFTGFGGNAVDFEDLLHEFSAVIETTDSPATVQAGVLRQARRLVPGYRIELITGPAP
                     SHDHATVIERDCEESIGGKSPPHSDAAQTGQLVLEVPLRCGSLVRGRLRIRSRSARPA
                     ALRSETVRRLNTLCTIGASALDRLCLQEEWPWDDNVAHLSDPAGADSDHGSAKTEGTF
                     PQSARLQDATFLNAVLPFALSQARRHLEPLSIVCVSIDRLRGIQDLLGRAEVDRLVRS
                     VGETVGSLIRTSDIVVRLDDDRVIAVLPRAPGKGALEVAKKICLAVAANTQRNCDITS
                     VTVSIGVATFPSCADNVLSLFSAADDALERAQSQGRNRAALAPPRSAPASSVSRAVST
                     RAS"
     gene            complement(1627..1953)
                     /locus_tag="VHS97_04210"
     CDS             complement(1627..1953)
                     /locus_tag="VHS97_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447428.1"
                     /translation="MTVSTFLIFIAAAALEVGGDALIFKGLRGRGLGLIALGCVALCC
                     YGLVVNLVKWDFAKLLGVYVAVFASLSVLTGRFALKEDVPLSTWMGLGLIVVGGVVIQ
                     FGKRSL"
     gene            2004..2951
                     /locus_tag="VHS97_04215"
     CDS             2004..2951
                     /locus_tag="VHS97_04215"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX3447429.1"
                     /translation="MSTRMRLPEINRYHDRTAPTPLVPVRLDDNGPTIWCKLEFLNPS
                     GSTKDRIATYILSKALRRGEIAPGGTVVEASSGSTSIAMALASAQLGLCFVAVMPEGV
                     SQERRMIIESYGGEVVFSPRADGIRGALAGAALEAVGRGGFAPLQFSNPDNPGAHRYR
                     TAHEIIAQIPGGCVDAVISGVGTGGTLVGLYQGFADFGCSVAPFAARPVSGKALDGAE
                     CCSFSGRIPGVADGLSAIYAAFQSESNNLVELDVADDEAIEVTRALIRKGFPVGPSSG
                     LNYRAAMMAASRLGPEAHIVTVFPDRMERYFSTELFSKH"
     gene            complement(2918..>3764)
                     /locus_tag="VHS97_04220"
     CDS             complement(2918..>3764)
                     /locus_tag="VHS97_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447430.1"
                     /translation="SKTETAKRVYPWAIEFYTFIGRRFSESEGISKEVLAKAHRQAGF
                     CRLNMGDSRGRDDYRHAIRGFQDLAARFPDRLWYRTRLIETLLEYAGLLPAPADAALA
                     DELFRSALPVAEALLGDKNADLHCYRIGLIGPFNDLAWKLVRRPPTRESDAALAIRLA
                     RKAVNCEPGQAAYWNTLGVAYYRQGDWSAASTSIEKSIELGNGGDATDWFVLASTLHR
                     QGRSDQARQWFDRAVQWVERNPGLDKVRAAEILGFRDEASRILGGQGPLSHGTQCLEN
                     NSVEK"
BASE COUNT          711 a         1148 c         1167 g          738 t
ORIGIN      
        1 aacacaagcc aggccgagct gcgcggcagc gaccaaacgt cgacttccca caatacggcc
       61 cgctgtgctc aatcggctcg actcacaagc agagcttgat gcgttttgcg ggttggaaca
      121 agatttgcgg ctgggccgcg gtcgcctatc ctgcgcgttc gcagtgggac cttaatggtg
      181 ctcgaacagg ctcagcgatt tcgtttggct gtcgctttgg gagcgcttat ctagacaaag
      241 atcgatggcc ttcaaactcc ttgattcgtg gcgtcggttc agccctgaca gcatgtggcc
      301 ttcatccttc atcaacctat caacatggtc aaacccaatg acacagacca cgatgactgc
      361 acgtacctgg cgatgtgatt ccgtcggagt tcctcaggcg ccaagcgata tgagcgggtc
      421 tgccgatctg tcggccggca gccatcggag ccctgaggaa tcggttaccg agccgatgcc
      481 gagtcatccc acgttcgggc tcatccggca ttggacccgc actgtgaaac gctggatgcg
      541 cgagagattc acgggcttcg gtggtaacgc cgtcgatttt gaagacctct tgcacgagtt
      601 cagcgccgtc atcgagacga cggacagtcc tgcgaccgtc caggcgggtg tgttgcgaca
      661 ggcgcggcgg ctggtgccgg gataccggat cgagctgatc actgggccgg caccgtccca
      721 cgatcacgcc accgttatag agcgagattg tgaggaatcg atcggtggaa agagtccgcc
      781 ccactctgac gccgcccaga ccggccaatt ggttctggaa gtgccgttgc gttgcggctc
      841 gttagtccgc ggccggctgc ggattcgctc gcgcagcgcg cgtccagccg cgctccgaag
      901 cgaaacggtg cggcgactga acaccctttg cactataggt gcgtccgcgc tcgacaggct
      961 gtgcttgcag gaggaatggc cctgggacga taacgtggcg cacctgagtg acccagctgg
     1021 agcggattct gaccatgggt ctgcaaagac cgagggcacg tttccgcagt ccgcccgatt
     1081 gcaagacgcc accttcctga atgccgtgct cccgttcgcg ctcagccagg cgcggcggca
     1141 cttggagcca ttgtcgatcg tgtgcgtgtc tatcgaccgt ctgcgcggaa ttcaagactt
     1201 gctgggccgg gccgaggttg accgtttggt ccgaagtgta ggcgaaacgg tcggctcttt
     1261 gatccgtacc agcgatatcg ttgtccggct cgacgacgat cgtgtcatcg cggtgctgcc
     1321 acgggccccc ggaaaaggcg cgctcgaagt cgccaagaaa atatgtctcg ctgtcgcagc
     1381 gaatacccag cgcaattgcg acatcaccag cgtcacggtc tcgatcggcg tggccacttt
     1441 cccctcttgt gccgataatg tcttatcgtt gtttagcgcg gccgatgatg cacttgagcg
     1501 ggcgcagagc cagggtcgaa accgggccgc ccttgccccc ccgcggtcgg ctcccgcgag
     1561 ttcggtctcg cgcgctgttt ctactcgggc gtcctgatcg gttgcaaact tctgcgttcc
     1621 caccggctag agcgatctct tgccaaactg gataactacg ccgccgacaa cgatgagacc
     1681 gagtcccatc caggtcgaga ggggcacgtc ttccttgagc gcgaagcggc cggtcaagac
     1741 gctcagcgaa gcaaaaacgg cgacgtagac ccccagaagt ttggcgaaat cccacttgac
     1801 caggttcacg accagaccgt agcagcagag ggcgacgcac cccagggcga tcaacccaag
     1861 gccgcgtcct ctgagtcctt tgaagataag cgcatcacca ccgacttcga gcgcggccgc
     1921 tgcaatgaag atgaggaaag tcgaaactgt catctctcgt cctttagaat tgtcatctct
     1981 tgttttttgg agggaactgg gccgtgagca cacggatgag actccccgag atcaatcgat
     2041 accacgatcg gacagcgccg acgccgctcg ttccagtgcg gctcgacgat aatggcccga
     2101 cgatctggtg caagctcgaa tttttgaacc cgagcgggtc caccaaggat cggatcgcga
     2161 cgtatatcct gagcaaggcc ttgcgccggg gcgagatcgc gcctggcggg actgtcgtgg
     2221 aagcctcgag cggctcgacg agcatcgcga tggcgctggc atctgcgcag ctcggtcttt
     2281 gtttcgtcgc cgtgatgccc gagggcgtca gtcaagaacg gcgaatgatc attgagtctt
     2341 acggcggcga ggtggttttc tcgcccaggg ccgacggcat caggggcgcc ctagcaggtg
     2401 ccgcgctcga ggcggtcggg cgtggcggct ttgccccact ccagttttcc aatccggaca
     2461 accccggcgc ccacagatac cggaccgcgc acgagatcat cgcgcagatc cctggcggat
     2521 gtgttgacgc cgtgatcagt ggagtgggaa ctggagggac gctggttggg ctctaccagg
     2581 gcttcgccga tttcggttgt tcggtcgccc cctttgccgc caggcccgta agcggtaaag
     2641 cgctcgatgg ggcggaatgc tgctcgttca gcggtcgcat accgggtgtg gccgacggtc
     2701 tgtcggcgat ctacgcggcg ttccaatccg agtcgaataa cctggtcgag ctcgacgtcg
     2761 ccgacgacga ggccatcgag gttacgcgtg cgttgattcg caagggtttc ccggtcggcc
     2821 ccagctcggg cctcaattat cgggcggcca tgatggccgc ttcccgcctc gggcccgagg
     2881 ctcatatcgt gacggtattc ccggaccgga tggagcgcta cttttcgacg gagttgttct
     2941 ccaagcactg agtgccgtgg gacaagggac cctgtcctcc tagaattcga ctcgcctcgt
     3001 cacggaaccc gaggatctcg gcggcccgga ccttgtccag gccgggattg cgctcgaccc
     3061 actgcacagc gcgatcgaac cattggcggg cctggtccga acggccctgc cggtgaagag
     3121 tggaagcgag gacgaaccag tccgtggcgt ctccgccgtt gccaagctcg atcgactttt
     3181 cgatcgacgt ggaggccgct gaccaatcgc cttggcggta ataggcgaca cccagcgtgt
     3241 tccaatacgc ggcttggccg ggctcacaat tcacggcttt gcgagctagc cgaatggcca
     3301 gagcagcgtc agattctcgc gtgggagggc gacggaccag cttccaggcc agatcgttaa
     3361 acggtccgat caggccgatc cgatagcagt gcaaatcggc gttcttgtca ccgagcagag
     3421 cctcggcgac cgggagtgcg gagcggaaca gctcatcggc tagggcggcg tccgcgggcg
     3481 ccggcagcag accggcatat tccagaagcg tctcgatcag acgagtccgg taccataacc
     3541 gatcggggaa tctcgccgcc agatcctgaa agccacgaat tgcgtgccga taatcgtcgc
     3601 gtccgcgcga atctcccatg ttcagtcgac agaaaccggc ctggcgatga gccttggcta
     3661 gcacctcttt tgaaatacct tcgctttccg aaaatcttcg gccgatgaac gtgtagaact
     3721 cgatcgccca gggatagact cgcttggccg tttcagtttt cgaa
//
LOCUS       DATAQL010000248         6729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_941509, whole genome shotgun sequence.
ACCESSION   DATAQL010000248 DATAQL010000000
VERSION     DATAQL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941509"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VHS97_04225"
     CDS             complement(<1..317)
                     /locus_tag="VHS97_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447431.1"
                     /translation="MKRNPVAWAALIVASAALFSSSGFLRQMPAAPKVTEEGQRTAKA
                     LSQAFGAVADFARPSVVQISVQKKMGRGMTLPPGLRRIPTPRGNPGNPGDPGQDLEEM
                     LRR"
     gene            807..1871
                     /locus_tag="VHS97_04230"
     CDS             807..1871
                     /locus_tag="VHS97_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX3447432.1"
                     /translation="MLDPRNLAPEIFATRARELGAHDLSIRRLIAATVGQGIHDPLIW
                     GREFQVPKRLRDAIAFPLPRLVLERSITSPVDGFQKLLFRTYEGLAVETVLIPLHKTG
                     AVSVCLSSQVGCPMGCVFCATARMMQRRNLETWEIIDQLLQAREIVRREGRRVTGAVF
                     MGMGEPFLNYDRVLAAAELLRCPFGGAVAAQAITISTVGLVPEIDRFTLERRRFRLAI
                     SLGAATDAKREQLVPLAARTPVAEVMAAARRHSLARRDRVMLAYVCIGGVNVDESDAR
                     ALADLIGDTAVRLDLIDVTDPTGRWHPPAPDEYRRFRDALACHLKQPVVRRYSGGADI
                     EAACGTLAGTQFSAVRQRHS"
     gene            complement(1905..2294)
                     /locus_tag="VHS97_04235"
     CDS             complement(1905..2294)
                     /locus_tag="VHS97_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447433.1"
                     /translation="MFAKLACHLPRFWVGKLAHYGQHRNDEHCVALFEEASRYTGLHL
                     ENDLADSGYWSMMPLQQRAIVLLFLVDRGLVNRTVRRGRRVFEPLPHAESWVESQPCL
                     HPYKKPTLELLAALRHELQRRAHSHHA"
     gene            2643..5030
                     /locus_tag="VHS97_04240"
     CDS             2643..5030
                     /locus_tag="VHS97_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3447434.1"
                     /translation="MFSALSRFVIYRPWVVILGWIILTAGLHQAAPRWEQVTKDDNVR
                     FFPPDSLSVIGQDLLERGFPADASSSQLVLVYERKHGHVTPEDLRYIEQVASSFYQFA
                     QSVSDLGVKSLDTHRSPVIGPRLIGTSADGRDQAVLTIVRLNGTYLSKKTRVAVDRIL
                     EWLRSERPPAPAGLELALTGSAVVGRDTNKAAAESINNTTNSTVALVIIILLVVYRSP
                     LLAMVPLVTIALSVFVSLRLIALLTTVPRLGFQVINITQVFVVVVLFGAGTDYCLFLI
                     ARYCEELGRGRSRVDALREAIGQVGAALVASAGTVIVGLGMLYFSTFAKVKYTGPTIA
                     IGLAVALLAALTLAPAMLAILRSTIFWPFHAPHRSAGADGGPDDLENGPVAGFWAGIA
                     DLVVSYPFAILSVCLLVLTPLAVVGARTKSNHNQLSDLDPDRPSVIGANVVTRYFAVG
                     ELSPAIALVSNPRLDFSAPSGRQAIDEINRRLLAIDNIAEVRSLTQPLGKAAVPVART
                     SGGFFDRLAGQAMTTGAESHYVSVRPLLASDRNHITRFDVVFKTDPFSESSLRALDRV
                     NEVFLAASVPGQPLEGTESIGIAGSSSSVHDLKRVTTTDEHRMYVLVTLGVYAVLVLL
                     LRRPGISLYLIATVVLGYLASLGLTDLVFQALHRGPDPWGGLDWTVGFFLFVILVAVG
                     EDYNILLMARVIEEERKYGTTDGTRRAVASTGGIISSCGLIMAGTFGSMLTGSLTSLR
                     ELGFALGLGVLLDTFLVRPILVPAFVVLLDRIKTGRRTVSGPAESQQLATPVELS"
     gene            complement(5275..>6729)
                     /locus_tag="VHS97_04245"
     CDS             complement(5275..>6729)
                     /locus_tag="VHS97_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX3447435.1"
                     /translation="DRYEAHHRVQITDEALEASVELSDRYITGRCLPDKAIDVVDEAG
                     ARVRLKAMTRPPDLKELDEQIERLNQDKEEAVANQDFERAASLRDSADKLKKKKETIT
                     REWRERSKEIDGTVDEEVVAEVVSKMTGIPLTRLETEETARLLRMEDDIQKKVISQSE
                     AIKRISEAVRRSRAGLKDPKRPIGSFIFAGPTGVGKTLLAKALAEFMFGDSDALIQID
                     MSEYMEKHNVSRLIGAPPGYVGYEEGGQLTEKIRRRPYAVVLLDEIEKAHPDVYNTLL
                     QIMEEGRLTDSFGRNVDFKNTIIIMTTNAGAEVTSYSNIFGFDRGRDEAGSYEQMKER
                     LKVAIEKYFRPEFLNRLDDVIVFHALNKEDLKRIVDIELAKIRSRMSERGMELVLTDE
                     AKDFLILKGYNPDYGARPLRRAIENLIENPLSEEILRNSFKGKDVVDVQVEGPPEARK
                     LKFVASAKQAPEEKENLVAVGSDKESAPPDAKNN"
BASE COUNT         1238 a         1968 c         1992 g         1531 t
ORIGIN      
        1 aacctccgca acatttcttc caggtcctgg ccgggatcac cggggttgcc agggttgcct
       61 cgcggggtcg gaatccggcg aagcccgggc ggcaaggtca tacctctgcc catcttcttc
      121 tgaacgctga tctgcacgac tgacggtcgc gcaaaatcgg ccacggcccc aaatgcctgc
      181 gataatgcct tggcggtgcg ctgcccctct tccgtaactt tcggcgccgc aggcatctgg
      241 cgcaaaaagc ccgatgagct gaagagtgcg gccgacgcca caatcaaagc ggcccatgca
      301 actggattgc gtttcatact gtttcgatct cctttgatga gcttctagtc ctcacgactg
      361 tgatcatggt gagcgaaaaa gagaaagtcg gccggtgaga agtgcgtact ctgactggat
      421 gtagtacatg ccgaccaacc tacgacgtcg agtttctcga cttccctcaa cctttaattc
      481 gctgcatctc tcattccaac cacctcgtcg cgctgtgatc cgtggcgaag cggttgtcac
      541 actaggtact tcagtattac gaagcggata ttcagaaggt tgggttcaaa tccccggctc
      601 atttttccat ccacgattga gtcagttcaa aggtacccgg tggggccgcg gtcaccggtc
      661 gccgcattgc gatttaacgg ccaacggact atcaaccgtc ccagcattgt cccacggcga
      721 ctatccgcgg ggagtcgctg gggatagtct aagaaaaagt cgtcgttcgt cgactgcttt
      781 ttgtctttcc tccaagagtc aagctcgtgc tggacccgcg caatctggcc cctgagatat
      841 tcgccacccg cgcccgtgaa ctcggcgcac acgatctgtc gatacgccgg ctgatcgccg
      901 cgacggtggg ccagggtatt catgatccat tgatatgggg ccgggagttt caagttccca
      961 agcggttgag ggatgcgatc gcattcccgc tccctcgctt ggtcttggag cgatcaatca
     1021 cttcgccggt ggacggattt cagaagctct tgttccgcac ctatgaagga cttgcggtcg
     1081 agacggtatt gatcccgtta cataaaacgg gtgcggttag tgtgtgtctg tcgtcccagg
     1141 tcggctgtcc gatgggttgt gttttctgcg cgacggcgcg gatgatgcaa cggcggaacc
     1201 tggaaacctg ggagatcatc gaccagttgc tgcaagcgcg cgagatcgtg cggcgagaag
     1261 gacgacgagt gacgggggcg gttttcatgg ggatgggtga gccattcctg aattacgacc
     1321 gcgtgctggc ggctgctgaa ttgcttcgtt gtccctttgg aggagcggtt gcggcccagg
     1381 cgatcacgat cagcacggtc ggcctggtgc ccgagatcga tcgattcacc cttgaacggc
     1441 gccgttttcg cctggcgatc agcttgggcg cggcgacaga cgccaagcgg gaacagttgg
     1501 ttcccctggc cgctcgcact cccgtcgccg aagtgatggc cgccgcccgc cggcactcgt
     1561 tggcccggcg agatcgggtg atgctcgctt acgtctgcat cggcggagtc aatgtcgacg
     1621 aatcggatgc tcgggcgctc gctgacttga tcggcgacac ggccgtgcgt ctcgacctga
     1681 ttgacgtaac ggatccgact gggcgctggc acccccctgc accggacgaa tacaggcgat
     1741 tccgcgatgc actcgcgtgc cacctgaagc agccagtagt tcgccgctac tccggcggag
     1801 ccgacatcga ggcggcatgc ggcacgcttg caggcacgca gttctctgcc gttagacagc
     1861 ggcattcctg acgatggctc agaaacgggt ggccaaccgg cggcttatgc atggtgcgaa
     1921 tgagctcgcc tctgaagctc gtgacgcagt gcggccagta gctcgagcgt gggctttttg
     1981 tagggatgca agcacggctg gctctcgacc cacgactcgg cgtgagggag cggctcgaaa
     2041 acgcgacggc ctctgcggac ggtgcggttg accagaccgc ggtccaccag aaacaagagc
     2101 acaatggcac gctgctgcag aggcatcatg gaccagtagc cggagtcggc cagatcattc
     2161 tccaggtgca agccggtgta gcgacttgct tcttcaaaaa gcgcaacaca atgctcgtca
     2221 ttgcggtgct ggccgtaatg agcaagctta ccaacccaga agcgaggcag gtgacaggcg
     2281 agttttgcga acacggactt aagtatcctc tcgggccgac aaaatcaaac atgtgactgc
     2341 aaaacagatc acactcttta attggtaggt catcctacgg cgtcgggcaa gtcccttgtg
     2401 ccggattggg cggttctagt ggcaaaaaca gctctacatg atcaggcgct gagacaggac
     2461 acgccgcgaa aaaggcgcgc atgagcacag tcggctttct ctttgacatg gggggcaacc
     2521 atcgactatg gtcggtgaac tgacaaccga ctttggagga cgacattgcg ttgccagacc
     2581 ctgcatcgac ccgaattact tgcttgatca cggatcgtct gagcccggaa cgctaacgac
     2641 ttatgttctc cgccctgtcc agatttgtca tctaccgccc ctgggtggtc atccttggtt
     2701 ggatcatcct gaccgcaggt cttcaccagg ccgccccgcg ctgggaacag gtgaccaagg
     2761 acgataacgt ccggttcttt ccccccgatt cactgagcgt cattggacaa gacttactcg
     2821 agcgcggctt tcccgcggat gcctcgagct ctcagcttgt cttggtttac gagcgcaagc
     2881 atggacatgt gacgccggag gatctgcggt atattgagca ggtggcctca agcttttacc
     2941 aatttgccca gtctgtctcc gatttgggag tgaaaagcct cgacacgcat cgctcgccgg
     3001 tgatcggtcc tcggctcatc ggtacgagtg cggacggtcg ggatcaggct gttctgacga
     3061 tcgttcgctt gaacgggacc tatctctcga aaaagacccg tgtcgccgtc gatcgaatcc
     3121 tggaatggct tcggtcagag cgaccgcccg cgccagcggg cttggagcta gcactgaccg
     3181 gttcggcggt ggttggacgc gacaccaata aggcggccgc cgagagcatc aacaacacga
     3241 cgaacagcac cgtcgccctg gtaattatca ttctgttggt tgtttaccgg tcaccgctgc
     3301 tggcgatggt cccgctggtc acgatcgcgc tgtcggtttt tgtatcgctc cgtctgatcg
     3361 cgctccttac gacggttccg aggttaggat ttcaggtgat caacatcacc caggttttcg
     3421 tcgtggtagt cctcttcggt gccggaaccg attactgcct gtttctgatc gcgcggtatt
     3481 gcgaggaatt gggccgtggc cgatcaaggg tcgacgctct gcgagaagcg atcggccagg
     3541 tgggagcggc cttggtcgcc agcgccggta ctgtgatcgt cggtctgggc atgctttatt
     3601 tctcgacttt cgccaaggtc aagtacaccg gtccgaccat cgctatcggg ttagcggtag
     3661 cgctccttgc tgctttgacc ctggcaccgg ccatgctcgc catattacga agcaccatct
     3721 tttggccgtt ccacgccccg catcgctcgg ccggggccga cggtggaccc gacgacttgg
     3781 agaatggtcc tgtcgccggt ttctgggccg gtattgcaga cttggtcgtc agctatccgt
     3841 tcgccattct ctcggtctgc ctgttggtct tgacgccgct cgccgtggtc ggggctcgaa
     3901 ccaaatcgaa ccacaaccag ttgagcgacc tcgaccccga tcggccgagc gtgatcggag
     3961 ccaacgtggt cacgcgctat ttcgccgtcg gcgagctcag cccggcgatt gcgctagtga
     4021 gcaatcctcg ccttgatttc tccgcaccca gcggacggca agcgattgat gaaatcaacc
     4081 ggcgattgct ggcaattgac aacatcgccg aggtccgttc gttgactcag cctctgggta
     4141 aggccgccgt ccccgttgct cgaaccagcg gcggtttttt cgatcgactg gccggtcaag
     4201 ccatgacaac gggggcggag tcgcattatg taagcgttcg gcctttgctg gcgtcagacc
     4261 gcaaccacat cacgcgcttc gacgtggtat tcaagaccga cccgttctcc gagtcgagcc
     4321 tccgtgctct cgatcgcgtc aacgaggtct ttctggccgc gtcggttccc ggacagccgc
     4381 tggaagggac cgagtcgatc gggatcgcgg gctcgtcgtc gtcggtccac gacctgaaac
     4441 gggtgaccac gaccgacgaa caccgaatgt atgtccttgt cacgcttggc gtttatgctg
     4501 tacttgtcct cttgctcaga cggcctggca tcagccttta cttgatcgcg accgtcgtgc
     4561 tgggatatct ggcatcgctc ggtctgaccg acctggtctt tcaggcactt catcgcggcc
     4621 ccgacccttg gggcggtctc gattggaccg tcgggttctt tctcttcgtc atcctggtcg
     4681 ctgtcggcga ggattataac atcctcttaa tggcacgcgt gatcgaggaa gagcgcaagt
     4741 acgggacgac ggacggcacg cgacgggccg tcgccagtac gggcggaatc atcagctcgt
     4801 gcgggttgat catggccggg acattcggat cgatgttgac cggtagcctc acttcgctgc
     4861 gtgagcttgg ctttgctttg gggctgggag tgttgctcga tacgtttctg gttcggccga
     4921 tcctcgtgcc tgccttcgtg gttctgctcg acagaatcaa aacgggtcga cgcaccgtgt
     4981 ctggtccagc ggagagccag cagttggcga ctccggtcga gctttcctga agccgaatcc
     5041 ggcgtcggga agcttggcgc cgtgccccag gaccaaggca atgcatcgaa aacatcgtac
     5101 tagttcatga caagatataa tcccatcccc ctttgtgggg gagggagaca gaactcgcct
     5161 tgacctaaca ggttgcacct aattcagcgg tgtaggcccc agggcggggt caaataaaaa
     5221 cgggtgagcc agatcccgat ccggctcacc cgtttgatca ttcagagttg cagttcagtt
     5281 attcttggcg tcgggtggcg ccgattcctt gtcactgccg acggcgacaa ggttttcctt
     5341 ttcctcgggt gcctgcttgg cgctggcgac gaacttgagc ttccgggcct caggcggccc
     5401 ttccacttgg acgtcgacaa cgtccttgcc tttaaacgag ttccgcagaa tctcctcgct
     5461 gaggggattc tcgatcaagt tctcgatcgc acggcggagt ggacgtgccc cgtaatcggg
     5521 gttgtagccc ttcaggatca agaaatcctt agcctcgtcg gtcaagacta gctccatgcc
     5581 gcgctcgctc atgcgggagc ggatcttggc cagctcgata tcgacgatcc gcttcagatc
     5641 ttctttattg agggcgtgga aaacgatgac atcatcgagc cggttcaaga actccggccg
     5701 gaagtatttt tcgatggcaa ccttgagccg ttctttcatc tgctcgtagc tgccagcttc
     5761 atcgcgtcct cggtcgaatc cgaagatgtt cgagtacgac gtgacctcgg ctcccgcgtt
     5821 ggtcgtcatg atgatgatcg tgttcttgaa atcgacattg cgaccgaagc tgtcggtcaa
     5881 ccgcccttct tccatgatct ggagcagggt gttgtagacg tccggatgcg ctttctcgat
     5941 ctcgtccaag agcacgacgg cgtaggggcg gcggcggatc ttttcggtca attggccccc
     6001 ttcctcatag ccgacgtagc caggaggggc gccgatcaat ctggagacat tgtgcttctc
     6061 catgtactcg gacatgtcga tttgaatgag ggcgtcggaa tcgccgaaca taaactcggc
     6121 taaagccttg gcgagcaagg tttttccgac tccggtcggt ccggcgaaga tgaagctgcc
     6181 gatcggccgc ttgggatcct tcaatcccgc gcggctgcgg cggacagctt cgctgatccg
     6241 cttgatcgcc tcgctctggg aaatgacttt cttctggata tcgtcttcca ttcgcaagag
     6301 ccgggccgtt tcttccgtct cgagtcgcgt cagggggatc ccggtcattt tgctaacgac
     6361 ctcggcgacg acttcctcgt cgacggtacc gtcgatttcc ttggagcgtt ctcgccattc
     6421 gcgagtgatt gtctcctttt tctttttgag cttgtcggcc gagtcgcgca gcgaggccgc
     6481 tcgctcaaag tcttgattgg cgaccgcctc ttccttgtct tgattcaacc gctcgatttg
     6541 ctcgtcaagt tctttgaggt caggcgggcg cgtcattgcc ttcaaccgaa cgcgcgcgcc
     6601 ggcctcgtcg acgacgtcga tggccttatc aggcaagcaa cggccagtga tgtaacgatc
     6661 ggacaactcg accgacgctt cgagggcctc gtcggtaatc tgaacgcggt ggtgcgcttc
     6721 ataacggtc
//
LOCUS       DATAQL010000249         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4910997, whole genome shotgun sequence.
ACCESSION   DATAQL010000249 DATAQL010000000
VERSION     DATAQL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4910997"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..3642
                     /locus_tag="VHS97_04250"
     CDS             478..3642
                     /locus_tag="VHS97_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3447436.1"
                     /translation="MHKKPSARLVAYILAVLAPALTLLALWPLPAALGDQVLYTAFFP
                     AVLIAAYLGGLGPGLLATVISALAATFFLVEPLYSFAIHSGSDAVALAVFLVVGVIIS
                     GLGESLQLARRRIVTVERQRAEVALRETEERFRSLAENIRDVFWIIDPRGDRVLYASP
                     GYEEVWGRTLQSLYEQPGSLVESIHAEDRDEAIAHLKQDRLGEFWVQEYRVVRPDGSV
                     RWIRARVFPIKDRAGRFAHRAGLAEDITDRKRAEEALRESEQRFRGTFEHAAVGIAHV
                     DAESRCLRANAKMCEILGYAGTELVGKTVPEVTHPDDLGPNLFWFGALMRGELANFTM
                     EKRFFHKDGSIVSTYLTVSLQRDLAGKAAYAIVMVQDISELKRLEGELRKSMETAEAA
                     NRAKDEFLANVSHEIRTPMNAILGMTELALDTPLAADQRQYLNTVRSAADNLLGILND
                     LLDFSKIEAGKLELNPADFSLRATLGDTVPTLATRAHKKGLELACHVQSHVPDALVGD
                     ASRLRQVLLNLIGNAIKFTEEGEVVVRVEVDDNAASDGQVGLRFAVSDTGIGIPPEKH
                     ETIFCAFEQEDTSTTRKYGGTGLGLTIATRLVALMGGTITVESQLGRGSTFSFTAQFG
                     RQEHPGDPGAARLPVLLHKLPVLIVDDNATNRRILEEWLRAWQMEPAAVGDGLAALDG
                     LWGAVSVGRPYALVLLDARMPDTDGLSLAARIRKRVELSATRIILLTSGDRPGDPFLA
                     RQLRIDAHLLKPVQQDELLETIYRVMSRARDEAPMAIRPASQPLTAPAPALTSAPLQI
                     LVAEDNEFSAQLLEQLLARRGHRVRLAKNGREALASAENGGFDLLIVDVHMPELDGFQ
                     VVQAIREREQAVGGHLPIIALTARSRKEDRERCLGAGMDDFLSKPVRAAELWEAIDRV
                     AGARPPAERAGPTLLDPRVLWEVCGGDAGILERIGQAFRARLPGRLDAVQDALREQDS
                     LRLREAAHKLHGMVAVFSTLAGAVASDLEDLAARGQLDEARPLVEQLETMADELTRLT
                     SSLSFETLRQQAEVLNKPERAAGT"
BASE COUNT          693 a         1142 c         1198 g          674 t
ORIGIN      
        1 gcggggaacg tctggcgcac ttgctcttgg atcacggccg ggtcgtcatc gatgagcaac
       61 agatgggcca tggattgctt gctcccgcta tgtcaggggg acggccacag aacccgctca
      121 ttgtcaaggc ggtgactcgg cgtgtccagc cttgagggag agacatgtaa cgatagcata
      181 acgacccgcg aacccttttg gcaccgcttt cgcatcggtg attggcgcag cgttgtcgat
      241 gcgatggggg tcgattactt tcgagaagca ggcccgtggg tgttgcagac tattgcggca
      301 ggagtcggcg gacctaatgg cgccgagtta ttttctcctc ggcccgatct cgaacaatcc
      361 cgacgatgat cacttacgaa gccgaagagt ttttaagcac aatctatatc tctatagcaa
      421 gatcttcgcg ataggcgcca agagcgacgg tgggcgtacg cgtcacatga ggttggcatg
      481 cacaaaaaac caagcgcccg gctcgtcgcc tacattctag cggttttggc gccagccctc
      541 acgctgttgg cgctgtggcc gctcccggcg gcgctcggag accaagtcct ttacacggcc
      601 ttctttccgg ccgtgttaat cgccgcctac ctaggcgggc tcgggcccgg tttgttagcg
      661 actgtaataa gcgctctagc tgcaaccttt tttctcgtcg aaccgcttta ttccttcgcg
      721 atccacagcg ggtccgacgc cgttgctctc gccgtcttcc tggtggtcgg cgtgatcatc
      781 agcggcttag gcgaatccct gcaactggcc cggcgccgca tcgtgacggt cgagcgccag
      841 cgggccgagg tggccctgcg cgaaaccgag gaacgcttcc ggagtcttgc ggagaatatt
      901 cgcgacgtct tctggatcat cgacccacgt ggcgatcggg tgctctatgc cagtccgggc
      961 tacgaagaag tctgggggcg aacgctccag agcctctacg aacaaccagg ttccttggtc
     1021 gagagcattc atgcggagga tcgggacgaa gcgatcgcgc atctcaaaca agatcggctg
     1081 ggcgagttct gggtccaaga gtaccgggta gtgcggccgg atggctcggt gcgatggatc
     1141 cgtgctcgcg tgttcccgat caaggaccgt gccggccgct tcgcacatcg tgcggggctg
     1201 gcggaagaca tcaccgaccg caagcgggcg gaggaggccc tgcgcgagag cgagcaacgt
     1261 ttccgcggca cgttcgagca tgcggccgtc ggcatcgctc atgtcgacgc cgagagccgt
     1321 tgcttgcgcg ccaacgcaaa gatgtgcgag atcctcggct atgccggcac ggagctagtc
     1381 ggcaagaccg tgccagaagt gacgcacccc gatgacctgg ggcccaacct cttctggttc
     1441 ggagccttga tgcggggcga gctggccaac ttcacgatgg aaaaacgctt cttccacaag
     1501 gacggttcca tcgtctcgac ctaccttact gtctcgctgc agcgcgacct agcagggaag
     1561 gcggcctatg ccatcgtgat ggtccaagac atttcagagc tcaaacggct ggagggcgag
     1621 ctgcgaaagt ccatggaaac tgccgaggcg gcaaaccggg ccaaggatga gtttttggcc
     1681 aatgtcagcc acgagatccg cacgccgatg aacgccatcc tgggcatgac tgagctggcc
     1741 ctcgacacgc ccctggccgc agaccagcgg caatacctga acacggtgag atccgcggcc
     1801 gacaatctgc tgggcatcct caatgatttg ctcgacttct ccaagatcga agccggcaag
     1861 ctcgagctca atcctgccga cttctcgttg cgggcgacgc tgggcgatac ggtgccgacc
     1921 ctcgcaacac gcgcccacaa gaagggtcta gagctggcgt gtcacgtgca gtcccacgtg
     1981 cccgacgcgc tggtcggaga cgcctcaagg ctacgccagg tattgctcaa cctaattggc
     2041 aacgccatca agttcaccga agagggcgag gtagtcgtac gggtggaagt cgacgacaac
     2101 gccgcgtcag acgggcaagt cggcctacgc tttgcggtga gcgacaccgg catcggcatc
     2161 ccgcccgaga agcacgaaac gatcttctgt gccttcgagc aggaagacac gtcgaccacg
     2221 cggaagtacg gcggtaccgg gcttggcttg accatcgcca ctcggctggt cgccttgatg
     2281 ggcgggacga tcaccgtgga gagccaactc ggccgcggca gcaccttttc gttcacggcc
     2341 caattcggcc gtcaggagca cccgggagat ccgggcgccg cccggctgcc tgtcctgctc
     2401 cacaagctgc cggtgctcat cgtcgatgac aatgccacca accgtcgtat tctggaagag
     2461 tggctgcgcg cctggcagat ggagcccgcg gcggttggtg acgggctggc ggccctcgac
     2521 ggactttggg gcgccgtcag tgtgggccga ccgtacgcgc tggtcctgct cgatgcccgc
     2581 atgcccgaca ctgacgggct gtcgctggcc gccaggatcc gcaagcgggt cgagttgtcc
     2641 gccacgcgga tcattctgct gacatcaggg gaccgaccgg gcgacccctt cctggctcgc
     2701 cagttgcgga tcgacgccca cctgctcaag ccggtccagc aggacgagct gctggagacg
     2761 atctaccgtg tgatgagccg ggccagagac gaggcaccga tggcgatccg gccagcttcg
     2821 caaccgctaa ccgccccggc gccggctctg acctcagctc cgctgcagat cctggtggct
     2881 gaggacaacg agtttagcgc ccaactcctg gagcaactcc tggcccgacg ggggcaccgc
     2941 gtgcggctgg cgaaaaacgg ccgagaggcg ttggcctcgg ccgagaacgg gggcttcgat
     3001 ctgctgatag tcgacgtcca catgccggaa ctggacggct tccaggtcgt ccaggcgatc
     3061 cgggaacgag agcaggccgt tggggggcat ctgcctatca tcgccttgac ggcgcgctcg
     3121 agaaaggaag accgcgaacg atgcctcggg gcgggaatgg atgattttct gtcgaagccg
     3181 gttcgcgccg ccgagctgtg ggaggcgatc gaccgggtcg cgggtgctcg ccccccggcc
     3241 gagcgagcgg ggccgaccct actcgacccg cgtgtgctgt gggaagtctg cgggggcgac
     3301 gccggcatcc ttgagaggat tggtcaggcg tttcgcgctc gtctgccggg tcgtctggac
     3361 gctgttcagg atgccttgcg agagcaagac tcgctcaggt tgcgggaagc ggctcacaag
     3421 ctgcatggga tggtggccgt tttttccact ttagccggtg cagtagcctc ggatctggaa
     3481 gatcttgccg cgcgaggtca gctcgacgag gcccggccgc ttgtcgagca gctcgaaacg
     3541 atggccgacg agctcacgcg tctgacaagc agcctctcgt tcgagactct gcgacaacaa
     3601 gccgaagttc tcaacaaacc cgagcgggca gcaggcacgt gagaccacca actgggagat
     3661 tgctggaaca catcgttccg gcctgatccg atgcccaagc cgagcgt
//
LOCUS       DATAQL010000250         6114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_484364, whole genome shotgun sequence.
ACCESSION   DATAQL010000250 DATAQL010000000
VERSION     DATAQL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484364"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VHS97_04255"
     CDS             <1..292
                     /locus_tag="VHS97_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447437.1"
                     /translation="KATARVVARLVFITKGRLSPTFVFRTGPRREIAHCRSLRVPGLS
                     ATQCSIVSGFDSSCNIDQADPGDTPADFRQRNGNGTRIYEYHIMLRAYRGGG"
     gene            295..1083
                     /locus_tag="VHS97_04260"
     CDS             295..1083
                     /locus_tag="VHS97_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX3447438.1"
                     /translation="MGLDLTLAVIILIAALRGWFQGFVSQAVRLGGIIACVYLAEPVR
                     DYAKPNILPHLPSIQPELADRLLWWVSAAVTYVVLVGAASLVIKMTRRPEIPGISQSG
                     RNDQFAGFLLGAAKGVLVAAFAIAGVQKYALDQIKTVSWGEEQVKTSWALKWSDAYQP
                     VPRIWSSPPVRHFVNYVERMGLSKPGEPSQVPAGLEDERESPPVRTASRPTEGEIARQ
                     HRSDDEQGPSVTSTPSPVEETPGRSAVPAEKSVDKLRSQSKEPN"
     gene            1420..2592
                     /locus_tag="VHS97_04265"
     CDS             1420..2592
                     /locus_tag="VHS97_04265"
                     /inference="COORDINATES: protein motif:HMM:NF005750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEX3447439.1"
                     /translation="MANVLCVLYEDPVTGYPASYARDEIPVLTNYPDGQALPAPARVD
                     FKPGAMLGSVSGELGLRPFLERAGHTFIVTSDKDGPDSVFEKELPDADIVISQPFWPA
                     YLTAERLARAPRLKLAITAGVGSDHVDLHSAMKRKITVAEVTYCNSISVAEHAVMQIL
                     ALVRNYLPSHEWVVKKGWNIADCVERAYDLEGMSVGIVAAGRIGLAVLRRLEPFDVKL
                     HYTDKHRLSNAIEEELGLTYHSSVESLVRVCDVISIHCPLHPETEHLVNDDMLRKMKR
                     GSYIVNTARGKICDRDAIVRALQSGRLAGYAGDVWFPQPPPPDHPWRTMPHEAMTPHV
                     SGTTLSAQARYAAGVREILENWFANRPIRDEYLIVDGGKLAGVGAHSYTDADTSGK"
     gene            2729..3880
                     /locus_tag="VHS97_04270"
     CDS             2729..3880
                     /locus_tag="VHS97_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3447440.1"
                     /translation="MAAAAGFAAATTAVLMGPKPAEAGDPSFMNNVPDPLVSGKELPT
                     FKFELEKSQGKVIGNSFGKEATVEQLPVSKGIAGVSMKLEPGAMRELHWHATAAEWAF
                     VIEGRVRTTVIDPQGNAETNDFGPGTVWYFPRGHGHMLECLGDKPTHFILIFDNGYFS
                     EFGTFSITDWIGHTPKPLLAKNFGVPESAFDGFPKDEVYFARGAVPPQEQSVPLQGRK
                     LPPQTHKFELLEQAPHALFKGGREWRVDSSRFPISKTITGVILDLDPGALRELHWHPT
                     ADEWQCVIEGDISVTMFGSHGRYRTETLSKGDVGYIPQGYGHSIENIGSKGCRVLIGF
                     NTGIYETIDLSQWIAGNPLDVLATNFSKPPSLFEQFPQRDVFIAPKDGP"
     gene            3913..4344
                     /locus_tag="VHS97_04275"
     CDS             3913..4344
                     /locus_tag="VHS97_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447441.1"
                     /translation="MSDLSRRGFVGSAGLAASLVGLTAGANDDAEEGLATARARLALA
                     RAALEVVRANVGRGQFSPGERDPISIWSRRRLEARLELSKTKADRVAAAQEHVDEMKQ
                     VEQLIVRLEQAGQVDLLGKMDAEYRRLEAQSWLEREKMAKA"
     gene            complement(4733..5821)
                     /gene="cydB"
                     /locus_tag="VHS97_04280"
     CDS             complement(4733..5821)
                     /gene="cydB"
                     /locus_tag="VHS97_04280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX3447442.1"
                     /translation="MEAAWFVIVSGMLAFYAVLDGFDFGVGAIHRIVARNDEERRTVL
                     AAIGPIWDGNEVWLIAAGGVLFMAFPNVYATAFSGFYMALIVVLWLLILRGVAIELRS
                     HQDHPLWREFWDTVFMLASALLAVVFGTTLGNLIRGVPLGEEGLRGMPLFTNFLIGRA
                     PGILDWYTGLVGLFTLAALSVHGALYLAWRTIGPVRERSVAFALEAWKAALVLWVAAT
                     AATAWVRPEVFTGLVARPWAFAFVALSIAGAWGAIRFMRSGRDLAAFLSSSAFLLGML
                     AATLAGQYPFWLRSTLDPSHSLTALNSASDHYGLRVALTWWPVGIALVAVYFTFLFRW
                     MRGKVGMESGPSYGSAIAGRVDSPGQRV"
     gene            complement(5832..>6114)
                     /locus_tag="VHS97_04285"
     CDS             complement(5832..>6114)
                     /locus_tag="VHS97_04285"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX3447443.1"
                     /translation="GRLATTRPMLWVLMLAFPFPYIATTAGWWTAELGRQPWIIHGLM
                     RTADAHSKLVNPGDVVFTVLGFAGLYLLLGMLFVIQVLKEINSGPAASH"
BASE COUNT         1208 a         1862 c         1958 g         1086 t
ORIGIN      
        1 gaaggccaca gcccgggtcg tcgctcgcct cgttttcatc acgaaaggac gcctttcccc
       61 cacgttcgtg ttccgcaccg gaccgcgtcg cgagatcgcg cactgccgat cgttgagagt
      121 gcccggtctc tcggccacgc agtgctcaat cgtctccggc tttgattcaa gttgcaacat
      181 agatcaagcc gatccaggcg atacgccagc cgattttcgg caaagaaatg gcaacggcac
      241 gcgaatctac gaataccaca tcatgctgcg agcgtaccgc ggagggggtt aacgatgggt
      301 ttggacctga cattggcagt catcatcctg attgccgcgc tccgaggctg gtttcagggg
      361 ttcgtcagtc aggcagtgcg gctcggtggc atcattgcat gcgtgtatct ggccgagcca
      421 gtgcgcgatt acgccaagcc caatatcctg ccacacttgc catcgatcca acccgagctg
      481 gctgatcggc tgttgtggtg ggtttcggcc gcggtcacct acgtggtatt ggtcggcgcg
      541 gcgtcgctcg tgatcaagat gactcgccgg ccggagattc ccggtatttc ccagtctggt
      601 cgcaacgacc agtttgccgg cttcttgctg ggtgcggcaa agggggtgct ggttgcggcg
      661 ttcgcgattg cgggcgttca aaaatacgca ctcgatcaga tcaagaccgt ctcatggggc
      721 gaagagcagg tgaagacgtc gtgggcattg aaatggagtg atgcgtacca gccggtcccc
      781 aggatctggt catcgccacc cgtccggcat tttgtcaatt atgttgaacg aatgggcctt
      841 agtaaacctg gcgaaccgtc gcaggtgccg gccggccttg aggatgaacg cgagtctcct
      901 cccgttcgca cggccagccg accaaccgag ggggagatcg cgaggcaaca tcggtctgac
      961 gacgaacaag ggccgtccgt gacctccact ccgtctcccg tcgaggaaac cccgggaaga
     1021 tcggccgtcc ccgccgaaaa atcggtcgac aagctccggt cacagtccaa ggaaccgaac
     1081 tgaccgcccg cgccgtgatc gtgacctatt gccgctcggt gcagcggctc gcgtccgagc
     1141 gtgatgatag catcaccggc acatcgcccc cgaccgggtc ggtcgcaagg gcagggtcaa
     1201 accgtccagt gacggaatta tatgacccct cattctccgc ggattgccgt tgtcgatgac
     1261 ggcggcggtc ggagcggtgg tcgtccgtcg tgcgacaatc gatgaagggt ttgacccgcc
     1321 atctccgcga catttatcgg tgtgactggt gtatgccgta catgtcagcc tgggcgaact
     1381 cgatcatccg gcaggctttg ttttcgagga gcatcagaga tggccaacgt cctttgtgtt
     1441 ttgtacgagg acccggtgac gggctatccg gcgtcgtacg caagagatga aatcccagta
     1501 ctgaccaatt atcccgacgg ccaggcgtta ccggcgcccg cgcgggtcga tttcaaaccg
     1561 ggtgcgatgc ttgggagcgt ctcgggcgag ctggggctgc gaccgttcct tgagcgcgcc
     1621 gggcacacct ttatcgtgac ctccgacaag gatggcccgg actctgtttt cgagaaggag
     1681 ttacccgatg cagatatcgt catctcccag ccgttctggc cggcctatct gaccgcggag
     1741 cggctggcga gggcgccgcg gctcaaattg gccatcaccg ccggcgtcgg ctccgatcac
     1801 gtcgacctgc actcagcgat gaagcgcaag atcacggtcg ccgaggtcac gtactgtaat
     1861 agcatcagtg tcgccgagca cgcggtcatg caaatcctcg cgcttgtccg gaattacttg
     1921 ccgtcgcatg aatgggttgt caagaaaggc tggaacatcg ccgactgcgt cgaacgggct
     1981 tacgacctgg aggggatgag cgtcggcatt gtcgccgccg gccggatcgg tttggccgtg
     2041 ctccggcgcc tagagccctt cgacgtgaaa ctgcattaca cagacaagca tcgcctgtcg
     2101 aatgcgatcg aggaggagtt ggggcttact tatcactcga gcgtggagtc gctggtccgg
     2161 gtttgcgacg tgatttccat tcactgcccg ctccatcccg agacggaaca ccttgtcaac
     2221 gacgacatgc tgcgcaaaat gaagcgcggc tcgtacatcg tgaataccgc ccgcggaaag
     2281 atctgcgacc gcgacgccat tgtccgcgcg cttcaatctg gacgactcgc cgggtacgcg
     2341 ggcgacgtgt ggtttcctca gccgccaccg cctgaccatc cctggcgcac tatgccgcat
     2401 gaagccatga caccgcacgt gtcagggacg acactgtcgg cccaggcccg ttacgccgcg
     2461 ggcgtgcgcg agatactgga gaactggttc gccaaccggc cgattcgcga cgaatacctg
     2521 atcgtagacg gaggaaaatt ggccggagtc ggtgctcatt cctacacaga cgccgacacg
     2581 tccggcaagt aggacctacc gatcatcgtc gacctggact ggccggcgaa agtccggtct
     2641 gagctcgtgt cgtactgctg tagagtctgt gcgctacgta aactatttaa aggaccatca
     2701 catggtaggt ttgccgagga gagatttcat ggcggccgct gccgggttcg cggccgccac
     2761 gaccgccgtg ctaatgggcc cgaagcctgc cgaggccggc gatccgagct tcatgaacaa
     2821 cgtccccgac cctctcgtct ccgggaaaga gttgccgacc ttcaagttcg aactggaaaa
     2881 gtcccaagga aaggtgatcg gcaacagttt tggcaaggag gcgacggtcg agcaactgcc
     2941 ggtctccaag gggatcgccg gcgtctcgat gaagcttgag ccgggcgcca tgcgcgagtt
     3001 gcactggcac gccaccgcgg ccgagtgggc gttcgtgatt gaggggagag tgcgcacgac
     3061 cgtcatagac ccgcagggga acgccgagac aaacgatttc ggtccgggga ccgtgtggta
     3121 tttcccacgc ggccacggcc acatgctcga atgcctgggg gataagccga cgcacttcat
     3181 cctgatcttc gacaacggct acttctcgga gttcggcacg ttcagcatca ccgactggat
     3241 cggccatacc ccgaagccgc tgttggcgaa gaacttcgga gtccccgagt cggccttcga
     3301 cgggttcccc aaggacgagg tgtactttgc ccgaggggcc gtgccgccgc aggagcagtc
     3361 ggtccccctc caggggcgga agctgccgcc gcagacacac aagtttgaac tcctcgaaca
     3421 agccccgcac gcgctcttca agggaggccg ggagtggcgg gtcgattcca gtcggttccc
     3481 gatctcgaag acgatcacgg gcgtgatact cgacctcgac ccgggggcgc tacgggagct
     3541 gcactggcac ccgaccgccg acgaatggca gtgcgtcatc gagggagata tcagtgttac
     3601 aatgttcggt tcgcacggcc gttaccggac cgagacgttg tccaaggggg atgtcggcta
     3661 cattccccag ggctacgggc actcgatcga aaacattggt tccaagggat gccgggtact
     3721 gatcgggttc aacaccggga tctacgagac aatcgacctt tcgcaatgga tcgccggtaa
     3781 cccgctcgac gtccttgcca ctaatttcag caagccgcca tccctgttcg agcagtttcc
     3841 acaacgcgac gtgttcatcg cccctaagga cgggccttga atgcctggag cattatcgca
     3901 ggaggagtga ccatgtcgga tctttcgcga cgcggattcg tgggctcggc cggtctggcg
     3961 gcgagcctcg tcggattgac cgcgggggca aatgatgacg cagaggaagg tttggcgacg
     4021 gcaagagccc ggctcgccct ggctcgtgcg gccctggagg tcgtccgcgc aaacgttggc
     4081 cgcgggcaat tcagccccgg agaacgcgac ccgatctcga tctggtcgcg acgacggctg
     4141 gaggcccggt tggaactgag caagacgaag gccgaccgag ttgccgccgc gcaagaacac
     4201 gtcgacgaga tgaagcaggt ggagcagctc atcgtccggt tggaacaggc cggtcaggtt
     4261 gatcttctag gcaagatgga cgccgaatat cggcggttgg aggcacagtc ctggctggag
     4321 cgggagaaaa tggcgaaagc gtaggtttcc attcggggca agatccgccc cggcgtcgaa
     4381 cgaaatcgac tcgtcatgag agacatccct tatgattcgc ctacaaggcc ggctgggctc
     4441 ccgtgtggcc ctcggggcgg gacgtaccag tcgcttcgag cagccaccca ctcgcagtag
     4501 cgcctctctt tcttcgaaca tgctcgtctt aagttcctgg cggatcgtcg gactcaggtc
     4561 attcttggtc tcgaacatcg gcggactccc cgaagttggc aaaggatcag cggggaacgg
     4621 attcgccatg tcgcacggat cagggtgttt tcgtttgcaa caaggggctt ctggacaaca
     4681 atcacggtgg ccgcgtctgt cacgtcacgt ctcaggagct cgaacatcca cgtcaaaccc
     4741 gttggcctgg cgaatcgacc cggccggcga tggccgaccc gtatgacggc ccggattcca
     4801 tgccgacctt tccccgcatc cagcgaaaca gaaaggtgaa atagacggcg accagcgcga
     4861 tcccgacggg ccaccaggtc agcgccacgc gcagcccgta gtggtcggag gccgagttca
     4921 aggcggtgag gctgtgggag gggtcgaggg tcgaccgcag ccagaacggg tattggcccg
     4981 cgagtgtcgc ggccagcatc ccgagcagga acgcggacga ggagaggaac gcggcgaggt
     5041 cacggccgct ccgcatgaac cggatggctc cccaggcccc agcgatcgag agcgcaacga
     5101 aggcgaaggc ccatgggcgg gcgacgagac ccgtgaagac ttcgggccgc acccaggcgg
     5161 tggcggccgt ggcggcgacc cacaggacga gcgcggcctt ccacgcctcg agtgcgaagg
     5221 ccacgctacg ctcccgcacg ggcccgatcg tcctccacgc gaggtacagg gcgccgtgga
     5281 ccgacagggc cgcgagtgtg aagaggccga cgagcccggt gtaccagtcg aggatccccg
     5341 gcgcgcgccc gatgaggaaa ttcgtaaaca gcggcatgcc tcggagccct tcttctccga
     5401 gcggaactcc gcgaatcaag ttgccgagcg tcgtgccgaa caccacggcg agaagtgccg
     5461 acgcgagcat gaagacggtg tcccagaatt cccgccagag ggggtgatcc tggtgcgagc
     5521 ggagctcgat cgccacgccg cgcaggatca gcagccagag tacgacgatc agggccatgt
     5581 agaatccgct gaacgcggtg gcgtacacat tcgggaaggc catgaacaac acgccgcccg
     5641 ccgcgatcag ccagacctca ttgccatccc agatcgggcc gatcgccgcc agcacggtcc
     5701 tgcgctcctc atcattacgg gcgacgatgc ggtggatggc gccgaccccg aagtcgaagc
     5761 cgtcgagcac agcgtagaat gcgagcatgc ccgagacgat cacgaaccag gctgcttcca
     5821 tggtcagtgt cctaatgcga cgcggccggg ccgctgttga tctctttgag gacctggatg
     5881 acgaagagca tgccgaggag caagtagagg ccggcgaagc ccagcacggt gaagacaaca
     5941 tcgccgggat tgacgagttt cgagtgggcg tctgccgtgc gcatcaggcc atggatgatc
     6001 cacggctggc gtcccagctc ggcggtccac cagccggcgg tcgtcgcgat gtaggggaac
     6061 gggaacgcca gcatcagcac ccagagcatg gggcgtgtgg tcgccagccg cccc
//
LOCUS       DATAQL010000251         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4757541, whole genome shotgun sequence.
ACCESSION   DATAQL010000251 DATAQL010000000
VERSION     DATAQL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4757541"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VHS97_04290"
     CDS             <1..374
                     /locus_tag="VHS97_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3447444.1"
                     /translation="LVEIDLLRGGAPMPLKSPPSPSDYRIVVCRAGRAKSAELYAFSC
                     TVPIPQIPIPLLPGDAEPILDLNAVLHALIDRARYDLIIDYCQPPQPPLPDQEASWAA
                     AIIARTTDQTPDSTAGKGIAP"
     gene            371..1819
                     /locus_tag="VHS97_04295"
     CDS             371..1819
                     /locus_tag="VHS97_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447445.1"
                     /translation="MNCQDHLYRNADPRQVSRRWFFEQCGVGLGLAALGQLFQDEGYA
                     ASPASASGVNPLAAKAPHYAPKAKRVLFLFMAGAPSHLELFDNKPQLAKFDGTLPPAD
                     LIKGYRAAFIKPNSKLLGPKFKFARHGQSGTELSELLPHLATVVDDIAVVKGMVTDAF
                     NHAPGQIMMSTGSQIFGRPSLGAWVGYGLGSESQDLPGFVVFSTGKKGPSGGNSNWGN
                     GFLPTVYQGVQFRTSGDPVLYLSNPRGVDPELQRESFEAVNKLNQLRLSDVGDPEIAT
                     RINSYEMAYRMQLSAPDVMDIGHEPAHILDMYGVQSGKPSFASTCVLARRLLERGVRF
                     VQIFHESWDQHGDLTRDIKHNCEETDQACAALVKDLKQRGLLEDTLVVWGGEFGRTPM
                     VEGGGNDGRDHHPNAFTMWMAGGGIKPGITYGQSDELGFQVVKDKVHVHDLHATLLHL
                     LGFDHTKLIYRFQGRDFRLTDVNGEIVKGLLA"
     gene            2161..>2438
                     /locus_tag="VHS97_04300"
     CDS             2161..>2438
                     /locus_tag="VHS97_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447446.1"
                     /translation="MSEQRRGLRPNVERLQGRVLCSGLLPGSSANALTALTLVNKTVS
                     LHGTLRGHLKSEVGVPDVGRTFSSSGSGFLAGLGRASVAGNLQTTGFIA"
BASE COUNT          487 a          733 c          724 g          494 t
ORIGIN      
        1 gtctggtgga aatcgacctc ttgcgtgggg gggcgccgat gccgcttaaa tccccgcctt
       61 ctccgagcga ctatcgcatc gtcgtctgcc gggcgggtcg cgccaagagc gcggagctat
      121 acgcgttttc gtgcacggtg ccgatcccgc agatcccaat acccctgtta ccaggggatg
      181 ccgagccaat tctcgatctt aacgccgtgc tccacgcgct gatcgacagg gcacgctatg
      241 atctgatcat cgattattgc caaccgcccc agccccctct gcccgatcaa gaagcctcct
      301 gggccgccgc aatcattgct cgtacgaccg atcaaactcc cgactcaacc gctggaaaag
      361 gaatcgcgcc atgaactgcc aggatcatct ttatcgtaac gccgacccgc ggcaagtcag
      421 ccggcgctgg tttttcgaac agtgcggggt tgggttgggc ttggcagcgc taggccagct
      481 tttccaagac gaaggatatg cggcatctcc ggccagcgct tcaggcgtca atccgctcgc
      541 cgcgaaagca ccacattatg cgcccaaggc caagcgggtg ttgtttctgt tcatggcggg
      601 agcgcccagt catctcgagc ttttcgacaa caaaccgcaa ctggcgaagt ttgatggtac
      661 gcttcctccc gccgatctga tcaaggggta ccgcgccgcg ttcatcaagc ccaattccaa
      721 gctgctcggg cctaaattca agtttgccag gcacggccag agcggaaccg agctctcgga
      781 gttgttgccg cacctggcga ccgtggtcga tgacatcgcg gtcgtcaaag ggatggtcac
      841 cgacgccttt aaccacgcgc ccgggcagat catgatgagt accggctcgc agatctttgg
      901 ccgtcccagc ctgggcgcct gggtcggata cggtctgggg agcgagtcgc aagacttgcc
      961 cggttttgtc gtattcagca cgggtaagaa ggggcccagc ggcggcaact ccaattgggg
     1021 aaatgggttc ctgcccaccg tctaccaagg ggttcagttc aggaccagcg gcgatccggt
     1081 cttgtatctt tccaacccgc ggggtgttga tcccgaactg cagcgcgagt cgttcgaggc
     1141 ggtcaacaag ctcaaccagt tgcggctctc cgacgtgggc gatcccgaga tcgcaacccg
     1201 aatcaactcg tacgaaatgg cgtaccgcat gcagctctcg gctccggatg tgatggacat
     1261 cggccatgag cccgcgcaca ttctcgacat gtacggagtc caatcgggca aaccgtcgtt
     1321 cgccagtacg tgcgttctgg cgcgccggct gcttgagcgc ggcgtgcggt ttgtgcagat
     1381 cttccacgag tcgtgggacc agcacggcga tcttacccgc gacatcaaac acaattgcga
     1441 ggagaccgac caggcctgcg ccgccctggt caaggacctg aaacagcggg ggctgctcga
     1501 agataccctg gtcgtctggg gaggcgagtt cgggcgcacg ccgatggtcg aggggggcgg
     1561 gaacgacggc cgcgatcatc atccaaacgc cttcaccatg tggatggccg gcggcggcat
     1621 caagccgggg atcacctacg gccaaagtga cgaactcggg ttccaggttg tcaaggacaa
     1681 agtgcacgta cacgacttgc acgcgacgct cttgcacctg cttggctttg accacaccaa
     1741 gctgatctac cgattccagg ggcgcgactt ccgattgacc gacgtgaacg gcgagatcgt
     1801 caagggactg ctggcttgat cgaccacagc gatcatctgt ttcctgccgc aattcagcta
     1861 aactctcgat cggcgcgaca gctgctgcga agcgggatga aatcgattaa ccgatcgcaa
     1921 tcctccttag gggcataacc aatcgctcgt aaggaagatc acgataacga tgcaatcgtt
     1981 caactcacgt cgtcaaactg taaatcttgt cgtcccgttg atttgcgacc agcctcggca
     2041 cgtgcactga ttgttgatat catagcggct tcggctgggt cgcggcgaag ggcgtctgac
     2101 tgcgggctcg atttacgacc atatttggtt ggtcacaaga tttcgataga aaggaagccg
     2161 atgagcgagc aacggcgtgg cctcagaccg aatgtggaac ggcttcaggg cagggttctt
     2221 tgttcgggac tactgcccgg ttcatcggcc aacgctctca cggctttgac cctcgtcaat
     2281 aaaacagtct cgttgcacgg cacgctccga gggcatctca aatcggaggt cggagttccg
     2341 gatgtggggc gcaccttttc ctcgtccggt tcgggcttct tggccggcct tgggcgcgcg
     2401 tccgtcgcgg ggaacttgca aacgaccggc tttatagc
//
LOCUS       DATAQL010000252         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3994781, whole genome shotgun sequence.
ACCESSION   DATAQL010000252 DATAQL010000000
VERSION     DATAQL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3994781"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VHS97_04305"
     CDS             <1..320
                     /locus_tag="VHS97_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447447.1"
                     /translation="AVRRRECHRHVGDFTLFWTGVYPEALAKLQGPSRADALVNYQQQ
                     GKRSYHLASTLSNSKDQGYVLRRLSDQYELCAFGLSRVRREWEKFEAEGPPPGGPSRP
                     MVV"
     gene            675..1481
                     /locus_tag="VHS97_04310"
     CDS             675..1481
                     /locus_tag="VHS97_04310"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3447448.1"
                     /translation="MVLPLGDLHKTEIVPVVTYALIAINVLVFLVQQQKGDEFTLAYA
                     ATPYEIWHNEDLDQPVVKMVPVLVRDPFGRGVRLEQRAQARPHVPCPIPVRWTLLTSM
                     FLHGSWMHLLGNMLYLWIVGDNVEEVLGSFRFLIVYFACGLVGSLAQIAVDPDSITPT
                     LGASGAIAGIMGAYVVWFPHHQIRVLLFRFITVLPAVIVIGGWIALQIWLGAGTIGRG
                     NGQGGVAYLAHVGGAITGIVVALLFYEDARYIKERNAAAQGWSVYEGPEY"
     gene            1664..3676
                     /gene="asnB"
                     /locus_tag="VHS97_04315"
     CDS             1664..3676
                     /gene="asnB"
                     /locus_tag="VHS97_04315"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX3447449.1"
                     /translation="MCGIAGAIDLNGRREFSARRLLAMTGAIAHRGPDDEQIHIEPGV
                     ALGARRLSIVDLAGGRQPISNEDGSIWVTQNGEIFEYPELQTELLARGHRLATRCDTE
                     LWAHLYEDLGEAFFEKARGQYAVALWDRTNQKIVLGRDRVGICPLYYTLADGWLLWGS
                     EIKALLASGLVDAKPDPKGIDLFFNTFCAGTSRTFFEGIKSIPPGHFLRVHDGRVELK
                     KYWDLDFPDAGAERRHDDPTPLVDELEFLMRQAVERRLRGDVPVVSYISGGLDSTVVL
                     GLSSRQRGSAVPSFTIGLDRAGPDERTHARESAQALGSALSTITMNRGDIVAAYPELI
                     RAAEGPVMDSSAACLMRLARTVHDQGYKVVLTGEGADEALAGYAWFKTQKIRDAVSFG
                     PVIPALKGARNLALASIGAAAHRPELLGIRGFRTAQQDVYDFMSQARSVMYSGALWDR
                     LAGHPAYDDLDLTNDRFSRWHPLNQSLYVGYKVMLPGLLLLGKGDRVAMNSSVEARYP
                     LLDEDVIAFCATIAPEYKLHGLTDKWLLRQVAARTLPARIARRKKTMFRASRSDAFLA
                     HERPRWVDQLLSPESLRATGWFDPDAITRERAAQVRYPRITPRRIIMDLSLTCVISTQ
                     LWHHIYLGGGLCELPTWEAISPRDREASSLDMAVSDHAVSLSGSAR"
     gene            complement(3746..>3951)
                     /locus_tag="VHS97_04320"
     CDS             complement(3746..>3951)
                     /locus_tag="VHS97_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447450.1"
                     /translation="SFFFATLAWLITPRFLFLAVQMYVTALLMARFMHWNNLIYGVSW
                     WLALIEVAWVVRRRERECSARTY"
BASE COUNT          748 a         1142 c         1238 g          823 t
ORIGIN      
        1 acgccgttcg ccgacgcgaa tgccatcgcc atgtcggaga tttcactctt ttttggacgg
       61 gtgtttatcc agaagccctg gccaaactgc aagggccgag cagagcggac gcgcttgtga
      121 actaccagca gcaaggcaag cggtcatatc atctggccag caccctaagt aacagcaaag
      181 atcagggtta cgtattgcgg cgcctgagcg accagtatga gctttgtgca ttcggcctat
      241 cacgtgtccg gcgagagtgg gaaaagtttg aagcggaagg accgccgccc gggggcccga
      301 gccggccgat ggtggtctga tttggccccc tcaagcacag agtcgtcccg caacctacgg
      361 cggtgtaaca gattcgccgc atctggatgg cgcgttgcct ctgtttgcga taatcccatg
      421 ttgtcggcgc gcagggcgtg tacgagcgct cttattgctg tttgctcttt tactgcctca
      481 gccttataac caaactggtt cgatgacgat ttggccgctc ccggtgtccc gcgattccgg
      541 agccgattgg gtcgcctcga tctcagctcg catctgcttc gatttcaagc gtccggtgcc
      601 gcgattgctg gcaatgggat acgtgtgtgc aactcgttct gcccagcccg ctactttgag
      661 cgtgagacct gaccatggtt cttccccttg gcgacctgca caaaaccgaa atcgttccgg
      721 tcgttaccta cgcgctgatt gcgatcaatg tgctggtgtt cttggtacag caacaaaaag
      781 gggacgagtt cacgctcgcg tacgcggcca ctccttatga gatctggcac aacgaagacc
      841 tggaccaacc ggtcgtcaag atggtgccgg tactagtgcg cgacccattc ggccgaggtg
      901 tacgcctcga gcaacgtgcg caggctcgtc cgcacgttcc ctgtccgatc ccggtgcggt
      961 ggacgttgct gacgtcgatg tttctccatg gaagctggat gcatctgctg gggaacatgc
     1021 tctatctgtg gatagttggc gacaatgtcg aggaagttct tgggtcattt cggtttctga
     1081 tcgtctattt tgcatgtggt ttggtcgggt cgctggccca aatcgccgtc gatcccgatt
     1141 cgatcacccc cacgctgggg gcctcgggtg ctatcgcggg gatcatgggc gcttatgtcg
     1201 tctggtttcc gcatcatcag attcgtgtgt tgctgttccg cttcatcacg gtgctgcccg
     1261 ccgtcattgt cattggcggc tggatcgccc tgcaaatctg gctgggggcc gggacgatcg
     1321 gtcgcggcaa cggacaagga ggcgtggcct acctcgccca tgttggcgga gccatcacag
     1381 gcattgtcgt cgccttgctg ttttatgagg acgctcgtta tatcaaggaa cgcaacgcag
     1441 ccgcgcaggg atggtcggtc tacgaggggc cggaatattg actgagtttt gggcgattgc
     1501 gggacgccat ttaggtcggg ctctctagcc tgacgtcgag ctggaaagcc cgacctacat
     1561 cggtggcgtg atatagttga gccactgtag tgaagtcttt ccactcgtgg ttcgactgcg
     1621 gccaaaagct gatgattaaa atcgttgtga agagagggtc accatgtgcg gcatcgccgg
     1681 ggcgatcgat ctgaacggtc ggcgcgagtt ttccgccaga cggctgctgg cgatgactgg
     1741 ggcgatcgcg caccggggac cggatgacga gcaaattcat atcgagccgg gcgtcgcgct
     1801 gggagcgcgc aggctgtcga tcgtcgatct ggcgggcggg cgccaaccga tcagcaatga
     1861 ggacggctcg atatgggtca ctcagaacgg cgagatcttc gagtatcccg aactacaaac
     1921 cgagctcttg gcccggggcc accggctggc aacgcgatgc gacaccgagt tgtgggctca
     1981 cctatacgaa gatctgggtg aagccttctt cgagaaggcg cgtggccagt acgcggtagc
     2041 gctgtgggat aggacaaacc agaagatcgt gctcgggagg gaccgagttg gcatctgccc
     2101 cctttactac actttggctg atggctggct actatggggc tcggagatca aggcgctcct
     2161 ggcgtcgggg ctcgtcgacg caaagcccga ccccaaggga atcgacctct tttttaacac
     2221 attttgcgcg ggcacttcgc gaacgttttt cgagggcatc aagtcgatcc ctccgggcca
     2281 ttttctgagg gtgcatgacg gccgggtcga gctcaagaaa tactgggacc tcgactttcc
     2341 ggatgctgga gcggagcggc ggcatgacga tccgactccg ctggtcgatg agctcgagtt
     2401 tctgatgcgg caggccgtcg agcgccggct gcggggcgac gtacccgtgg tgagctatat
     2461 cagcggaggc ctggactcga cggtggtcct gggtctgagc tcgcggcagc gcggcagtgc
     2521 cgtcccgtcg ttcacgatcg ggctggaccg ggccggcccg gacgagcgca cgcacgcacg
     2581 cgagtcggcc caagcactcg gctcagcgct ttcaaccata acgatgaatc ggggcgatat
     2641 cgtggccgcc tatcccgagc tgatccgggc ggctgagggg ccggtcatgg attcgtccgc
     2701 cgcctgtctg atgcggctgg cgcgcaccgt ccatgaccaa gggtataagg tcgtattgac
     2761 cggcgaaggg gccgacgaag ccttggccgg ctacgcctgg ttcaagaccc aaaaaattcg
     2821 cgatgccgtg agctttggtc cggtgatccc ggctctcaag ggagcgcgaa acctcgctct
     2881 ggcatcgatt ggcgccgctg cccatcggcc cgagctgctg ggaattcgcg gattccgcac
     2941 ggcccaacaa gatgtttacg atttcatgag tcaagctcgg tcggtgatgt actcaggggc
     3001 gctctgggac cggctggccg gccaccctgc ctacgacgac ctggatttga ccaacgacag
     3061 gttcagccgc tggcatccat tgaaccagtc attgtatgtc ggctacaagg tgatgttgcc
     3121 aggcctcttg cttctgggca agggggaccg ggtggcaatg aactcatcgg tcgaagcacg
     3181 ctatccactt ttggatgaag acgtgatcgc attttgcgcg acaatcgcgc ctgagtacaa
     3241 gttacacgga ctcacggaca aatggctgct gcgccaggtg gccgcgcgta cgctgcctgc
     3301 ccggatcgcg cgccgcaaga agacgatgtt tcgggccagc cgctcggatg ccttcctcgc
     3361 acacgagaga ccgcgctggg tcgatcagct cttgagtcct gaatcactgc gggccaccgg
     3421 ctggtttgat cccgacgcga taacacgcga gcgtgccgct caggtccgat acccgcggat
     3481 cacccccagg cggatcatca tggacctgag cttgacctgc gtgatctcga ctcagctctg
     3541 gcaccatatt tacctgggag gcggcctgtg cgagctaccg acctgggaag ccatctcgcc
     3601 acgcgaccgc gaggcatcga gcctggatat ggccgtatcc gaccacgcgg tctcgctctc
     3661 cggatcggcg cggtgaatcg tgcagatcga ttccgttacc ttgtatcgag cgactgtctg
     3721 aagtcgcgcg tacagccgaa acgagctaat acgtccgcgc actgcattcc cgttcgcgcc
     3781 tccgaaccac ccaggcaacc tcaatcaagg ccagccacca agacacgccg tagatcaagt
     3841 tattccagtg catgaaacgg gccatcagga gggccgtcac gtacatttga acggcgagga
     3901 acaggaaccg aggagtgatg agccaggcca gggtggcgaa gaagaaactg c
//
LOCUS       DATAQL010000253         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4604994, whole genome shotgun sequence.
ACCESSION   DATAQL010000253 DATAQL010000000
VERSION     DATAQL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4604994"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VHS97_04325"
     CDS             complement(<1..1351)
                     /locus_tag="VHS97_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3447451.1"
                     /translation="MLTVGSAVRLAPAADEGRKPTQTGRIPWTTSRVVGSPDPPPPFK
                     VVRAFPNLTFRHPVLIARYPEGNRLVVGEQAGILYSFPDRRDARSDLFLDLPRELRTV
                     HQLAGAKEVEAVYGLAFHPDFERNRQCFVCYTIRGSDPRRQNLVDGTRVSRFLVTRAD
                     PPRIDPSSEEIVISFLQGGHNGGDLHFGPDGMLYISTGDAANPNPPDPFNTGQDISDL
                     LSSILRIDVDRRDQGKNYAIPKDNPFTATEGARPEVWAYGLRNPWRMSFDRKTGELFV
                     GDVGWELWESVHRVERGGNYGWSAMEGPQPIKSGQVGPTPIRPPLIELPHTIACSVTG
                     GRVYRGRRFPALQGAYVFGDWETRRLWAARFEGDRTVEMAEIARPSVRFVAFGEDRDG
                     ELLFVDEDGGTLHTLEKGDDGARNADFPATLSKTGLFASVDGHRPANGVIPFAVNGRQ
                     "
     gene            complement(1767..>2062)
                     /locus_tag="VHS97_04330"
     CDS             complement(1767..>2062)
                     /locus_tag="VHS97_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447452.1"
                     /translation="KSRTMELINLRPGRAEFNRASPTTQPIAIPRSAVHVVRPSFKLF
                     RGGPIHRTPRKHVNISRTPASNVSQIQAASRFRARWGSKPVWQAEGHASLRQP"
BASE COUNT          366 a          667 c          669 g          360 t
ORIGIN      
        1 actgccggcc attcaccgcg aacgggatca ctccgttcgc cggcctatgg ccgtcgaccg
       61 acgcgaacaa tccggtcttc gacagcgtgg ccgggaagtc ggcgttgcgg gcgccgtcgt
      121 cgcccttttc taaggtgtgc agcgtgccgc cgtcctcgtc gacaaagagg agctcgccgt
      181 cgcggtcttc gccgaaggcg acgaagcgca ccgacggccg tgcgatttcc gccatctcca
      241 cggtgcggtc tccctcgaag cgcgccgccc agagtcgtcg cgtctcccag tcgccgaaga
      301 cgtaggcccc ttgcagcgcg gggaatctcc tgccgcgata gacccgaccg ccggtgacgc
      361 tacacgcgat cgtgtgcggc agctcgatca agggcggccg gatcggggtc ggcccgacct
      421 gacccgactt gatcggctgc ggcccctcca tcgcggacca gccatagttg ccgcccctct
      481 cgacccgatg gaccgactcc cacagctccc aacccacgtc gccgacgaac agctcgccgg
      541 tcttgcgatc gaagctcatc cgccagggat tgcgcaagcc atacgcccag acctctggcc
      601 gggcgccctc cgtcgcggtg aaggggttgt ccttggggat ggcatagttc ttcccttggt
      661 ccctccggtc cacgtcgatc cgcaggatgg atgagagcag gtcggagatg tcctgccccg
      721 tgttgaacgg gtcgggcggg tttggattcg ctgcgtcgcc cgtggatatg tagagcatgc
      781 cgtcggggcc gaagtggagg tcccctccat tgtggccgcc ttgcaggaag gagatgacga
      841 tctcctcgct cgacgggtcg atccgtggcg ggtcggcccg ggtgaccaag aaccgggata
      901 cccgcgtgcc gtcaacgaga ttctgccggc gagggtccga cccccgaatg gtatagcaga
      961 caaagcattg acggttgcgc tcgaagtccg ggtggaaagc cagcccgtac accgcctcga
     1021 cctccttcgc ccccgcaagc tggtgaaccg ttctgagttc cctgggcagg tcgaggaaga
     1081 ggtcggaccg ggcatcccgg cggtcgggaa aggaatacag gatgcccgcc tgttcgccga
     1141 cgaccagccg gttgccctcg ggatagcgtg cgatgagcac ggggtgccgg aacgtcagat
     1201 tcggaaaggc ccgcacgacc ttgaagggcg gcggcggatc cggagatccc acgacacgag
     1261 aggtcgtcca gggaatccgg cctgtttggg tgggcttgcg cccttcgtcc gcggccgggg
     1321 cgagcctgac cgcggatccg accgtcagca agatcgagag cgcggtcgtg agccgacgaa
     1381 tcacaccaag gccctctgac gacatgagca ttgctccgaa aaaagatact ggccgggcct
     1441 gcaacagagg gccattgtat cccctctcgc agggaagccg agaggggacc ctgcgggcga
     1501 gcagaagcaa aggcgtgcac ctcatgccgc cgaccaccgc gccggtcagc cgggatttgc
     1561 tcgacgagac actggtctgg atcctcaccg acgagaaggg acgacgggcc tagcggacaa
     1621 cacaccgatc cgggggcgtg ctacggtcgg agttcgaaca ttccgcctcg cgcttgagcg
     1681 acttaccgac tgcagtcatc gcccgctgat ccgaatcgat tcgctgtaaa cgggttagcg
     1741 tcgtttacat catcctcact acctgatcac ggctggcgga gcgaggcgtg tccttccgct
     1801 tgccataccg gcttcgagcc ccagcgtgcc cgaaatcggc tcgccgcttg gatctggcta
     1861 acattcgatg cgggagttcg cgagatgttg acgtgcttac gcggagtccg gtggatcggc
     1921 cctccccgaa aaagcttgaa actaggtcgg acaacgtgga cggcgctccg gggtatcgca
     1981 atcggctggg ttgttggaga agcgcgattg aactcggcgc ggcctggtct caaattgatc
     2041 aactccatcg tcctgctttt tc
//
LOCUS       DATAQL010000254         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_178364, whole genome shotgun sequence.
ACCESSION   DATAQL010000254 DATAQL010000000
VERSION     DATAQL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178364"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VHS97_04335"
     CDS             complement(<1..215)
                     /locus_tag="VHS97_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447453.1"
                     /translation="MSELVQFVVLQRSEKWVVKSRELERAFGDRRKAIHCAVELANES
                     GKNGKPALVLAGSRRHEFAPVWTFGKDP"
     gene            complement(540..1073)
                     /locus_tag="VHS97_04340"
     CDS             complement(540..1073)
                     /locus_tag="VHS97_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX3447454.1"
                     /translation="MPNLAKLPAWAGDELVHVVVETPRGARAKLEFDPGLKTFVLSKA
                     LMLGLSYPYDWGFIPSTRAEDGDPLDVMVIHDAATTPGLVLRCKIIGVLQTIQKTKRN
                     RIRNDRVIAVPKDSHRERELQGVADLPPEVKEELEKFFVATDELEAKTLKFLGWQGPA
                     QAKKLVRRCERVFKKKN"
     gene            complement(1222..1395)
                     /locus_tag="VHS97_04345"
     CDS             complement(1222..1395)
                     /locus_tag="VHS97_04345"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX3447455.1"
                     /translation="MDKDRIKGSAQQAKGKIKEVAGKVTGDKKLEGEGKADKVGGKIR
                     NVVGGMKDALRGR"
     gene            complement(1470..>1932)
                     /locus_tag="VHS97_04350"
     CDS             complement(1470..>1932)
                     /locus_tag="VHS97_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX3447456.1"
                     /translation="DISTSSAAAEALRPVAGTFAFTLFALGIVGTGLLAVPVLAGSAA
                     YAIGEARRWPVGLGRKPQKAKAFYATLTIATMVGVALNFSPINPIKALYWSAVINGVV
                     AVPVMVAMMHMTANPKVMGKFPVHDGLRLVGWMATAVMAAAAIAMGVTAVA"
BASE COUNT          343 a          640 c          568 g          381 t
ORIGIN      
        1 ggatccttgc cgaaggtcca aaccggcgca aattcatggc ggcgggagcc ggccaatacc
       61 agcgccggct tcccgttctt tccgctttca tttgcaagtt cgacggcgca gtgaatggct
      121 ttgcgccgat cgccgaacgc gcgctccaac tcgcgggact tcacgaccca cttttcactc
      181 cgttgcagca cgacgaactg cacgagctcg gacatctagt gaagtccgcc aaacagccaa
      241 atcagaagga tgatcggaat cgggattccg attagccaca acagaagacc gcggcccatg
      301 atgttctcct cttcaacact ttgcatcggc tgaacgcacg gccgatgaca aagttcccgc
      361 gcctgaccgg agccgatgaa tttctcaccg atcctgctgg gcatcattgg ccggcggccg
      421 ttcaacccgc gctcggacgt tcaacgaagg ttggaggatg acggggtttg gtacgctacc
      481 gaacattcgc gatctccgca gacactcgcg atctctgcag atgggtcgtc ggtgacaccc
      541 taattctttt tcttgaaaac tctttcgcaa cgccggacca gttttttcgc ctgcgccggt
      601 ccctgccagc cgaggaattt caacgtcttg gcttccagct cgtcggtggc cacgaagaat
      661 ttttccagct cttccttgac ctcgggcggc aggtccgcaa cgccttgcag ctcccgctcg
      721 cggtgcgaat ccttgggaac ggcgatgacg cgatcattgc ggattcggtt gcgcttggtt
      781 ttttgaatcg tttgaaggac gccgatgatc ttgcagcgca gcaccagccc cggcgtggtg
      841 gccgcgtcgt gaatgaccat gacgtcgagc ggatcgccat cctcggctcg ggtcgacggg
      901 atgaagcccc agtcgtaggg ataagacagg ccgagcatca acgccttgga caaaacgaag
      961 gtcttgagcc cgggatcgaa ttcgagcttc gcccgtgctc cgcgcggcgt ttcgaccacc
     1021 acgtgcacga gctcgtcccc ggcccaagcg ggtaacttcg caaggtttgg catcgcagtg
     1081 ctaacgggct actgccgcgc gacgggcgcg ccgcggtgca tcgctggtcc gcgtccgccc
     1141 gctggacttc gcgcagcagg acgcggcgat cccactttgg cggccggctg ctggcggccg
     1201 gcttgcggca agcgcagcgg atcatcgtcc gcgcagcgcg tccttcatgc cgccgacgac
     1261 gttgcggatt ttcccgccga ccttgtcggc ctttccttcg ccttcgagtt tcttgtcgcc
     1321 ggtcaccttg ccggcgacct ccttgatctt acccttggcc tgttgagccg aacccttgat
     1381 gcgatccttg tccattgcat gtctccgttc gttgtgacct ttagaacggt gctgccgcga
     1441 caaggttccc gggacacgaa gccacatcgt caagcgacgg ccgtcacgcc catcgcgatc
     1501 gcggccgccg ccatgaccgc cgtcgccatc cagccaacga ggcgcaggcc gtcatggacg
     1561 ggaaatttcc ccatgacctt cggattggcg gtcatgtgca tcatggccac catcaccggc
     1621 accgccacca cgccgttgat caccgcgctc caatagagcg ccttgatcgg gttgatcggg
     1681 gaaaagttga gcgcgacccc gaccatggtc gcgatcgtga gcgtcgcata gaacgccttg
     1741 gccttttgcg gcttgcggcc gagaccgacg ggccaccggc gcgcctcgcc gatcgcatag
     1801 gcggccgacc ccgccaatac tgggaccgcg agcaggccgg tcccgacgat tccgagggcg
     1861 aacagggtga acgcaaacgt cccagccacc ggccgcagcg cctcggctgc cgcactcgag
     1921 gtcgagatgt cg
//
LOCUS       DATAQL010000255         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1705952, whole genome shotgun sequence.
ACCESSION   DATAQL010000255 DATAQL010000000
VERSION     DATAQL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705952"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VHS97_04355"
     CDS             complement(<1..1071)
                     /locus_tag="VHS97_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3447457.1"
                     /translation="MDRLPREASRAQVILAVLAVIAALYMLKAILIPVAVALVVSCML
                     SPVASFLRRHFPYGPLGAFALLLLSVVSGLYLASLMAESLMMATYKLPSDLERLAGQV
                     SGRITDLIRDQPSFRSFLPEPGTIDRLGDTNRALLIEKLSYGLTDFTTWVAQGFVILV
                     LVIFLLIESEMLTAKVIRFFARQPAEAKAAGSVLTLITRKIRAYLIARTLINAGLGVV
                     IALALWSLNVQFALTLGLVAGITNFIPYLGQLIGGALPTAIVLGQTGSMGHALAVAGI
                     YLAVVGIEGYVVTPLVMGRSLDLNGTTVLLACLFWGYLWGLIGLVLAMPITVSLKVVL
                     QTIPELNRWAELMSLDWQSPAPA"
     gene            complement(1112..2104)
                     /locus_tag="VHS97_04360"
     CDS             complement(1112..2104)
                     /locus_tag="VHS97_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447458.1"
                     /translation="MAPTVRKLHHVIGLLLALSAFFIWFDPRRAPVLVFVCLSTSGLV
                     WRDAWRGASGTALRPALLWAALALVLSASAQAIAVTEPFADGRPLTGRLTYIAVLALL
                     AALISVLNARTPGERVWALLMALLILVFLIPWLEGSGRMRRVSGLASLYIDSPWTLFY
                     GTLAVVGVTNYLPTKFGWAAACLGAVFILEYLGLTRVDWPPELRALVWECVSWTVALS
                     AWVARWIAGRTPNAQGFERLWFWFRDHWGVVWALRTQERFNRSAELARWPVRLSWFGL
                     VASGASQEGSRLDDPAAAATFRGLIRRFAQAWRLDQVAELGPTTSCDGGDAGRR"
     gene            complement(2168..>2622)
                     /locus_tag="VHS97_04365"
     CDS             complement(2168..>2622)
                     /locus_tag="VHS97_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX3447459.1"
                     /translation="GAVLAVVMQLAGVSALAFAVGVYLPLATTLPIFVGGALRGLVDR
                     TRKLTPEESETSPGTLMSTGLIAGGSLAGIIIALLVVFESFGKKLDYSIPATAGGEPG
                     YLIPVIAAFAVMAAALLAVAMLGKRPVAGAPGKPDDEGLGDIGLQEGP"
BASE COUNT          583 a          866 c          780 g          393 t
ORIGIN      
        1 cgccggcgcc ggcgactgcc agtcgagcga catcagctcg gcccaacgat tcagctcggg
       61 gatggtctgc aataccactt tcaggctaac tgtgatcggc atagccagca ccaggccgat
      121 caggccccag agatatcccc aaaaaagaca cgcgagcaac accgtggtgc cattcaagtc
      181 gagcgaccgc cccatcacca gcggcgtgac gacatagccc tcgatgccga ccacagccag
      241 gtagatgccc gctacggcaa gcgcatggcc catggaaccg gtctgtccca gcacgatcgc
      301 cgttgggagt gctccgccga tcaactggcc cagatagggg atgaaattgg tgatgccggc
      361 caccagcccc aacgtcagcg caaattggac attgagcgac cagagcgcca gcgcgatcac
      421 gacgcccagg cccgcattga tcaaggtccg ggcaatcaag taggcccgga tcttgcgagt
      481 aatcagtgtt agtaccgagc cggcggcctt ggcctcggcg ggctgacggg caaagaagcg
      541 gatcaccttt gccgtgagca tctcactttc gatcagcaaa aagatcacga ggaccaggat
      601 gacgaatccc tgggccaccc acgtcgtgaa gtcggtcagc ccgtaactca gcttttcgat
      661 caagagcgca cggttcgtgt cgcccagccg atcgatcgtg ccgggttccg gtagaaagga
      721 acggaatgac ggctgatcac ggatcaggtc ggtgattcgt ccacttacct ggcctgcaag
      781 gcgctccaag tccgagggca atttgtaggt cgccatcatc aggctctccg ccatcaagct
      841 cgcgagatag agaccgctca cgaccgagag caagagaagc gcaaaggccc ccagcggtcc
      901 ataaggaaaa tgccggcgca ggaagctcgc gaccggagag agcatgcacg agacgacgag
      961 cgcgacagcg accggaatca ggatcgcctt gagcatgtag agagcggcga ttacggccag
     1021 tactgccaga atcacttggg ctctggatgc ttctcgcggc agacgatcca cgtggtcccc
     1081 cgtgtcggct cgtcacggaa cttgtaagga ttcatcttcg tccggcatcg ccgccatcgc
     1141 aagacgtcgt gggacccagt tcggctactt ggtcgagtcg ccaggcttgc gcgaatcgcc
     1201 gtatcaagcc ccgaaacgtc gccgcggcag ctggatcatc cagccgcgac ccttcctggg
     1261 atgcaccgct tgccaccagg ccgaaccaag atagccgcac cggccatcgc gccagctcag
     1321 cggaacgatt gaaccgctct tgagtgcgca atgcccagac gaccccccag tggtcgcgaa
     1381 accaaaacca gagacgctca aagccttgtg cgttcggcgt gcggccggca atccagcgcg
     1441 cgacccaggc tgatagcgcc accgtccacg aaacgcactc ccacacgaga gctcttagtt
     1501 cgggtggcca atcgacccgc gtcaggccga gatactcgag tatgaagaca gcacccaggc
     1561 aggcggccgc ccagccaaac ttggtcggca gatagttcgt cactccgacg acggccagcg
     1621 tcccgtaaaa caaagtccac ggcgaatcga tgtaaagcga ggccagaccg gatacccggc
     1681 gcattctgcc ggatccctcc agccagggaa tcaagaacac gaggatcagg agagccatca
     1741 atagagccca tactctctcg cccggcgtgc gcgcattgag aacggagatc aaagccgcca
     1801 ggagcgcgag tacggcgata tacgtgagcc ggccggtcag gggcctgccg tccgcaaaag
     1861 gctcggtcac agcgatcgcc tgcgcactcg ctgacagcac aagcgccagc gccgcccaga
     1921 gcaacgcggg ccggagcgcc gtgccgctgg ctccgcgcca cgcgtcccgc caaaccaggc
     1981 ccgacgtcga gaggcagacg aacaccaaca caggcgcgcg ccgcgggtca aaccagatga
     2041 agaatgccga cagggccagc agcaggccga tcacgtggtg gagctttctg accgttgggg
     2101 ccattcggtc cgcgacaacg acattgctag cggccttact caggtcgggc gattcgttcg
     2161 cttgttgcta cgggccctct tggaggccga tgtcgccgag cccctcatcg tcaggcttcc
     2221 cgggcgctcc cgcgaccggc cgcttgccca gcatcgccac cgccaagagc gccgccgcca
     2281 tcaccgcaaa cgcggcgatg accggaatca gatatccagg ctcgcctcca gcggttgctg
     2341 gtatcgagta gtcgagcttc ttgccgaagc tttcaaagac gaccaaaagc gcgatgatga
     2401 tgcctgctag tgagccgccg gcgatcaggc cggtcgacat caacgtacct gggctggtct
     2461 cggactcttc gggcgtgagc ttgcgggtgc gatcaaccag accgcggagg gcgcccccga
     2521 cgaaaatcgg caatgtggtc gcaagcggta aatacacgcc cacggcaaat gccagcgccg
     2581 atacccccgc cagttgcatc acgacggcca gcaccgcccc ca
//
LOCUS       DATAQL010000256         5357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_178461, whole genome shotgun sequence.
ACCESSION   DATAQL010000256 DATAQL010000000
VERSION     DATAQL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178461"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VHS97_04370"
     CDS             <1..1226
                     /locus_tag="VHS97_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3447460.1"
                     /translation="TSNDSTARLWETATGNPLGAPLRHGDRVKSVTFSPDRTTFLTTA
                     RDNTVRIWEVAANSSVKAALPHDESFFPLSFSADRQTIMTRDADQSVRIRNAVSGKLS
                     GEPLRHEFRVIAGAFSPDHKSAVTAEEKTNAGPWETTTTRRWDVATGKPVGLPLEHGG
                     VNSVTYSPDGKTALTGGGGGALLWDATTGSRRKDKLFSGSNRVHAVAYSPDGKTILTG
                     DTEPIVRLWDDAGNQRIVEPMKHANMVLSVAYSGDGHTILTGSSDNTARFWDPATGKQ
                     LGPPMTHQGRVNAVAFSLDSRTALTGSRDTTARLWDVATGKPIGPPLPHSGPVLHVAF
                     WHDGKRVLTAGEVDVAQFWQLSPPVEGDAAQLQLWTQVVTGTELEVSGGVRVLDATTW
                     LERRHKLTDSGLHSP"
     gene            1599..3266
                     /locus_tag="VHS97_04375"
     CDS             1599..3266
                     /locus_tag="VHS97_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-arginine deiminase family protein"
                     /protein_id="HEX3447461.1"
                     /translation="MTSLISRWIWLGLSLITADESAASPQPQQILRVFADLKHTGILT
                     GPLTSSSPAENEFGIAVPLPTVNTGRDRWPEKLDAADQVVKRLGRLRVETTEAAGDVV
                     VSIESDSRPCLRLFRNTASGWRRQAPSEDSSWTLAPGKDGVVELGVGVVIPDARAAAA
                     PAVWPREFTVEISATSENSMRLRVPFRVAPYVIPSALEPVDELMIVSQAVTADSVHSV
                     RAFAARTGLKLVAHEVDERCDQWMQDTIEPGLFAFPTALGSSQARACLSGLRKESGSE
                     AARLDYQVARRLRRRGVVTVAPAIPRKQAGWNDWYGNVEATPPHTDRQGRRFPYGRVI
                     TGKHHDATMHPGVLKFLEAQGLQWPPIVVDTSWLAIGHVDEVVNFVPAKTKKGFKVLL
                     PSPNAARDMLEALLAKGLEEEPVFAATEDEMTLGALRMTIAQTTENLAIDETVAHLRE
                     QLKSELNLENSDFVMVPALFQGGMAVIPNAVNSVVVNGYLLVPEPLGPQLNEVDGFEQ
                     AIRAALAGCDVRVVFIDSWNAYHASGGEVHCGTNTFRRLRDPAWWTR"
     gene            3571..>5357
                     /locus_tag="VHS97_04380"
     CDS             3571..>5357
                     /locus_tag="VHS97_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3447462.1"
                     /translation="MGMRRPVRVLLAALMVAPVLTQVTPRARAQSTPTVDESFFVETV
                     YPLLHAAQCERCHSDNGVASETRLEFPEPGAGSAQIKAFGLSLIDLVDRRNPEQSLLL
                     RKPTKRVKHTGGQRVKPQSDDEKVLLSWINYLAGLSDEAVRQARENITRAERHGPMGP
                     TVRRLTHSQYNNTVRDLLGDQTEPASGFPKEDFINGFKNQVEGQGVSPLQAEAYSKAA
                     ERLALAAFRGGDHHRLISHQPQSATDARWIDPFVREFGLKAFRRPLTDQEVGRYSSLF
                     HMEVGRTKDLHGGASMVIEAMLQSPHFLFRLEGGEGGPDTRFAIASRLSYFLWNTMPD
                     DDLLRAAGRGELATMEQIEATARRMVEDPRAKSAMDEFLAQWMRFDRVRDATRDRRRF
                     REFNPEVAAAMMEETRRLFNHLVWSDQNFMEFFTAPYTFVNADLARLYDLPAPDEEFA
                     KVAYPAGSGRAGVLGHGAFLLLTSKPAETSPTARGLFVRNQFLAQEIPPPPAGVNAVL
                     ATVSENAPLTNRQRLAVHLNSESCASCHRMIDPIGLAFEQYNAIGVFQKKMVLQFPGP
                     GEGQTRGRTPMIKELDVDTSGFVHGMEDS"
BASE COUNT         1092 a         1616 c         1683 g          966 t
ORIGIN      
        1 cgacgagcaa cgatagtacg gcccgcttgt gggagacggc cacaggcaat ccgctcggcg
       61 caccgctgcg ccacggcgat cgggtcaagt cggtgacctt cagtcccgac cggacaactt
      121 tcctgaccac ggccagggac aatacagtgc ggatctggga ggtcgctgcc aattcctcgg
      181 tcaaggctgc cctaccgcac gacgaatcgt tctttccttt gtccttcagc gccgaccgcc
      241 agaccatcat gactcgggac gcggaccaat cggtccggat tcggaacgca gtgagcggga
      301 aattgagtgg cgagcccctg cgacacgaat tccgggtgat cgcgggtgcc ttcagtcccg
      361 atcataagag cgccgttact gcggaagaga aaacgaacgc ggggccctgg gaaacaacca
      421 cgacccggcg ctgggacgtg gcgaccggca aaccggtcgg gctaccgctg gagcatggcg
      481 gggtcaattc cgtaacctac agccccgatg gcaagaccgc acttaccggc ggaggtggtg
      541 gagcgctgct ctgggacgcg acaaccggct cgagaaggaa agacaaactg ttttcaggta
      601 gcaatcgggt ccatgccgtg gcttacagcc ctgatgggaa gaccattctg accggagaca
      661 cggaacccat agtccgcctc tgggacgacg ccggcaacca acggatcgtc gagccaatga
      721 agcatgcgaa catggtcctt tccgtggcgt acagcggcga cggccacacc atcctcaccg
      781 gaagctcgga caacaccgcc cggttctggg atccagcaac cgggaaacag ctcggcccgc
      841 ctatgacaca tcaaggccgg gtgaatgccg tggccttcag tcttgacagc aggaccgcat
      901 tgaccgggag tcgcgacacg accgctcggc tctgggacgt ggcgactggc aaacccatcg
      961 gtcctcccct tccccactcg ggaccggtcc tccacgtggc attttggcac gacggcaaga
     1021 gggtcttgac cgcgggagaa gtcgacgtcg cccagttctg gcaactgtca ccgcccgtgg
     1081 agggcgatgc agcacaactc caactctgga cccaagtcgt cactgggacg gagctggaag
     1141 ttagcggtgg agtacgcgtg ttagatgcca cgacctggct ggagcggcgc cacaaactga
     1201 cggattccgg cctccacagc ccgtaggttc aggttagggc gctttgtttt cgcttggcac
     1261 cctccttgaa caccggcacc gcagtcatcg tcatcgccgg cggcggtcac caaaccttga
     1321 acgtgggagg tgagagcgcc gagttcgtcc ccctcttccc ccctcttcta caattacggc
     1381 atcaacaccg tcatacttcg gaaccggctg tgtgccgacg gctacgttgc cgtggttgac
     1441 gctgttcacg acgcacgact cacgatcgaa ggacagccaa attattgggc ttgacgaggt
     1501 taccccgtgg ggagatcgaa tagagtgcgg aaacgatggt gccgggatgg ttcccgccgt
     1561 agggaagttc cctcgcatac gaccgtggaa agccgctcat gacctctctc atttcgcgtt
     1621 ggatctggct gggattgtcc ctcatcacgg cggatgaatc ggctgcatcc ccgcaaccac
     1681 agcaaatcct ccgtgttttt gcagacctta aacacacagg gattctcaca gggccgttga
     1741 catcttcatc gcccgccgag aacgaattcg gcatcgcggt tccgcttccg actgtaaata
     1801 ccggtcgtga tcgatggccc gaaaagcttg acgccgccga tcaggtagta aaacggctgg
     1861 ggcgattgcg tgtcgagaca actgaggccg cgggcgacgt cgtcgtttcc atagaaagcg
     1921 atagtcgccc ttgcttgcgc ctctttcgca ataccgcgag cgggtggcga cggcaggcgc
     1981 cgagcgagga cagctcgtgg acattggcgc cgggaaagga cggcgtggtg gaactgggcg
     2041 taggcgtcgt gatacccgac gcgagggccg ccgccgctcc ggcggtctgg ccgcgggaat
     2101 tcaccgttga gatatcggct acgtcagaaa atagtatgcg gctgcgcgtg ccgtttcgcg
     2161 tggcgcccta cgtcataccc tcggcacttg agccggtcga cgagcttatg atcgtctcgc
     2221 aagccgtcac ggctgattcg gtgcattcag tgcgggcgtt tgcggcacga acagggctca
     2281 agctcgttgc tcacgaggtc gacgaaaggt gcgaccagtg gatgcaagac accatcgaac
     2341 cgggactttt cgcattcccc accgccctag gcagcagtca ggcgcgcgcc tgcctgagcg
     2401 ggctgcgcaa agagtcgggg tccgaggcag ccagactcga ctatcaagtc gccaggcggc
     2461 tgcgccgccg cggagtggtg accgtggcgc cggcaatccc ccgcaaacaa gcgggttgga
     2521 acgactggta cggcaacgtc gaagccacgc ctccccacac cgaccgccag gggcgaagat
     2581 ttccttacgg ccgggtgatc accggaaagc accacgacgc cacgatgcac cccggtgtac
     2641 tgaaattcct ggaggcgcaa gggctgcaat ggccgccgat cgttgtggac acctcgtggc
     2701 tggcgatcgg acatgtcgat gaggtcgtga acttcgtgcc ggcgaagact aagaaggggt
     2761 tcaaggtgct tttgcccagc cccaatgcgg cccgagatat gctcgaggcg cttctggcga
     2821 aagggttgga agaggagccg gtttttgcgg cgacggaaga cgagatgacg ctcggcgcgc
     2881 tgcggatgac aatcgcccag acgacggaga accttgcgat cgacgagacg gttgcccacc
     2941 tccgcgagca actgaagtcg gaattgaatc tggagaattc cgactttgta atggtgcccg
     3001 ctttgtttca gggaggaatg gccgtgatcc cgaatgcagt caacagtgta gtcgtgaacg
     3061 gttatttact ggtcccggag cccctaggcc cccaactgaa cgaggtggac ggcttcgagc
     3121 aagcaatccg cgcagctctc gccgggtgcg acgtgcgggt ggtcttcatc gattcatgga
     3181 acgcctacca cgcgtcgggt ggcgaggtac actgcggcac gaacaccttc cgccgcctgc
     3241 gcgacccggc gtggtggacg cgctgacaac cgatcttgaa gcggccggct gaaccgggag
     3301 gtttgccagg tcgggcggaa atgacaggcg gcgcaaacca agccgttact cggtgggcgg
     3361 atcaggcaca tgtatgcatc ggaggtataa ccagttgcac aacctgggtt gaagtattgt
     3421 cgaacattgt ttgcacgtga tatgatcggt tcgcgatcag agagccaaag ctgtctagga
     3481 atacctattg acagcgccaa cggcaggcac aaattggctc gtcaccggat tcgacttcag
     3541 gccagcttca tccaacgcgg gaggaacttc atgggaatgc gacggcccgt gcgggtgctg
     3601 ttggccgcct tgatggtcgc tcccgttttg acccaagtca caccgcgcgc acgtgcgcag
     3661 tcaactccta ccgtagatga gtcgttcttc gtcgagacgg tgtacccgct gctccacgcc
     3721 gctcagtgcg aacggtgcca cagcgacaac ggtgtcgcct ctgagacacg actcgaattc
     3781 cccgagcccg gcgcaggttc ggctcagatc aaggcgttcg gactttcgct catcgatctg
     3841 gtcgaccggc ggaatccgga gcaatcgctg ttgctgcgga agccgacgaa acgggtgaaa
     3901 cacacgggcg gccagcgtgt caaaccgcag agtgacgacg aaaaggtatt gctgagctgg
     3961 atcaactacc tggctggcct ttctgatgaa gcggtgcgac aggcgcgaga aaacatcacg
     4021 cgggccgagc gacacggtcc gatggggccg acggtgcgtc ggctgacgca cagccaatac
     4081 aacaacacgg tccgtgacct gctcggagat caaaccgagc ccgccagcgg ttttccgaaa
     4141 gaggacttca tcaacggctt caagaaccag gtggagggac agggggtctc accgctccag
     4201 gcggaagcct acagcaaggc cgccgagcgg ctcgccctgg ccgcctttcg aggcggagat
     4261 catcaccgat tgatctcgca ccagccccaa tcagcgaccg acgcacgttg gatagacccg
     4321 ttcgtgcggg agttcggcct gaaggcgttc cgtcgcccct tgacggacca ggaagtcggc
     4381 cgatattcgt ccctgttcca catggaagtc ggccgcacga aagacctcca cggcggcgcc
     4441 tcgatggtca ttgaggcgat gctccagtca ccacatttcc tgttccgctt agaagggggc
     4501 gagggtggcc ccgacacacg gttcgcgatc gccagccgac tgtcgtactt cctctggaac
     4561 acgatgcctg atgacgacct tctgcgcgct gccggccggg gagagctcgc aacgatggaa
     4621 cagatcgagg cgactgcgcg acgcatggtc gaggatcccc gtgccaagag cgccatggac
     4681 gagttcctgg cgcagtggat gcgcttcgac cgcgtgcgcg atgccacgcg cgatcgccgc
     4741 cggtttcgcg agttcaaccc cgaagtcgcc gcggcgatga tggaggagac gcgtcgctta
     4801 ttcaaccatc tggtctggag cgatcagaac ttcatggagt tcttcacggc tccctatacc
     4861 ttcgtgaatg cggatctcgc acggctctac gatttgccgg ccccagacga ggaattcgcg
     4921 aaggtcgcgt acccggcggg ttccggccgt gccggcgtgc tggggcatgg cgcgttcttg
     4981 ttgttgacca gtaagcccgc agaaacgtcg ccgaccgcgc gagggttgtt cgtgcgaaac
     5041 cagtttctcg ctcaggaaat tcctccgccc ccggcgggag tgaacgctgt cctcgccact
     5101 gtcagcgaaa atgcgccgct gacaaaccgg caacggctgg ccgttcactt gaacagcgag
     5161 tcgtgcgcga gttgccatcg tatgatcgat cccatcggac tggcttttga gcaatacaac
     5221 gcgatcggcg tatttcagaa gaagatggtg cttcagtttc cgggaccagg ggagggtcag
     5281 acccgcgggc gcacgccgat gataaaagag ctggacgtcg acacgtccgg gttcgtccat
     5341 gggatggaag attcgga
//
LOCUS       DATAQL010000257         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1094773, whole genome shotgun sequence.
ACCESSION   DATAQL010000257 DATAQL010000000
VERSION     DATAQL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094773"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1410
                     /locus_tag="VHS97_04385"
     CDS             307..1410
                     /locus_tag="VHS97_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447463.1"
                     /translation="MNLMTLVRLLVVVVLGWTVVALGVGVFGVGTNESTGATFYVPNP
                     SLVDTFTLGRLDHPSSVQHRLIDRVTGRVEPLSLPQDKAWSLLSVSPWRDRHGFSMAV
                     GRWISRLEGQEEFCGLGLLKLPGGTLENCLSLEVLPTGKPCWVPGQTDEILFPAGDGQ
                     LYRCNIAGNAKDDGNDGSHGTSRGDGGNLVAPRVVTWGTKRPGSGDVFLCDPAWSAEP
                     ALRNFIFVSLSEQKYLGTARMNSAFKLWSLLMNDAGDTIVAAERLTRPDSDKDTNDSL
                     SERMPTIVVGSGGQISLVYLAREPRAQSWQLRMVKLQTDHDQSLPKIGPGSTNSSVVA
                     DGMAAFALVVSADAKLVHGIDISGAHREVAIAR"
     gene            1570..3870
                     /locus_tag="VHS97_04390"
     CDS             1570..3870
                     /locus_tag="VHS97_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX3447464.1"
                     /translation="MRRVFRLSKSCVIIVVSVIAQLACSAQCPAAVTREEVERAIRDG
                     VRYLKGLQHPNGSWSDVENEAKTGTTSLITLALLTAGESPESQAIRKALEYLRGFGPN
                     DLHSTYAVSLQTMVFAAAEPERDQLRIANNVSWLERAQIKPGDPVYWPGSWTYSDSKR
                     GRPGDNSNTQYALLGLFAASEVGVPVKPMVWELSRLYWEKSQKRDGSWGYTPDVSNST
                     ASMTCAGISSLIITGLRRFQGQEFLQGDSIENCGKGGMNRNLQAGIDWLANRFQVGQN
                     FGHGQQWKYYYLYGLERAGRLAGVRFFGQNDWYRLGAEELVHEQHKLGGFWMGALNES
                     EKVLATSFAILFLAKGRAPVLINKLRHEPVGDWNNDPDDVRNMVGIVSRDWKSLLTWQ
                     VVEPGIATVSELLQAPIVYFNGHHAPQFSQLARRNLREYVEQGGFIFAEACCGKPEFD
                     QGFKQLMKEIFPEEEYKLRPLSEDHPVWRARHMLAPDLHPLWGIEHGCRTVVIYSPAD
                     LSCYWNQSEQSPLNTAVMKSIKVGQNVIDYATGREMPADKLTIRDVRDFKLDAPKRNA
                     LRIAKLKHAGDWNVAPQAIPNLMDALRKPPFRFDVVVTQKDLYPRDPNLVYYPLVYIH
                     GRAALSFSKEDMDALHQHLDPGGGTLFADAACGSPAFDAAFRHFAAEMFPNNPLVPIP
                     RDDQLFTVQVGADLSDVQYTKGAGGGRDFPQLEGVKINDHWVIIYSKYDIGCALERHT
                     GIECKGYTYESALKIAGNIVIYSTLP"
BASE COUNT          803 a         1206 c         1198 g          807 t
ORIGIN      
        1 gcccctcctc ttccgggagt ggccagcgta ggcccgcgct ggccgtgaaa tttgtaaccc
       61 aggatacgcc gagattccga atgtcgctga tttcctaata gaggtccggg attcagaatc
      121 tttgcctcac gacggcgtgg cttgaggtcc tttagtctcg tcggccttgt ctcgcgcgct
      181 tggattggca aagcgccgtg caacttttca gaccgccgcg tcgaccgcac ggcggcggtg
      241 atcgagatcg tcggttgatc gctgcgttgg acgcagctgg aaatcttatt aaatggagaa
      301 acgcagatga accttatgac tttggtccga ctgttggttg tagtggtgct cggctggaca
      361 gtggtcgcgc tcggcgtcgg agttttcggc gtgggaacga acgaaagtac gggcgccacg
      421 ttctacgtcc ccaatccgtc cctcgtcgac accttcacgc tgggcaggct cgatcatccc
      481 agcagcgttc aacatcggct gattgatcgg gtgacggggc gcgtcgagcc gctgtcgctt
      541 ccgcaggata aggcttggtc attgctgagc gtttccccct ggcgtgatcg acacgggttt
      601 tcaatggcgg tcgggcgctg gatcagccgg ctggaaggac aagaggagtt ttgcgggctg
      661 ggtcttctca agctgcccgg tggaaccctc gagaattgcc tctcgctcga agtcctcccc
      721 actggcaagc catgctgggt acccggccag accgatgaaa tactgtttcc ggccggagac
      781 gggcaactct atcggtgtaa catcgccggt aatgccaagg acgacgggaa tgacggtagc
      841 cacggaacct cccgtggcga tggcggcaat ttggtcgcgc cacgcgtggt gacctggggc
      901 accaaacggc cgggctctgg tgatgtgttc ctttgcgacc cggcctggtc ggccgagcca
      961 gccctccgca attttatctt cgtgtcgttg agcgaacaaa aatacctggg aaccgcacgg
     1021 atgaacagcg cgttcaagct atggtcgctg ctcatgaacg acgcggggga tacgatcgtg
     1081 gccgcggaaa ggttgactcg acccgattcg gacaaggata cgaatgacag cctatccgag
     1141 cgcatgccca ccatcgtggt gggctctggt ggtcagatca gtctcgtcta tttggcccgt
     1201 gaaccacgtg cacaatcgtg gcaacttcgg atggtcaagc tccagaccga ccatgatcaa
     1261 tcgctgccga aaatcgggcc cggatcaacg aactctagcg tcgtcgccga tggcatggcg
     1321 gcatttgctc tcgtggtgtc cgccgacgcg aagctcgtcc atgggatcga tatctccggc
     1381 gcgcaccgcg aggtcgcgat cgcccggtga tcgccaacga caagaacatg ccaagagccc
     1441 gttgccgcgg cgcgacagcg ctccccggcc cggcccatct tgtcgcttga tggcttcgtg
     1501 tgtcatgcta gacacaggaa tgcccatgtc gtcgccatgc ctctggactc gaccgcggga
     1561 ggatcgccag tgcgtcgcgt tttcaggctc tcgaagtcct gcgtgatcat tgtcgtttcg
     1621 gtcatcgccc aattggcctg tagcgcccag tgcccggccg ccgtgacccg cgaggaggtc
     1681 gagcgggcga ttcgcgatgg ggtgcggtac ttgaaggggc tgcagcatcc caacggctca
     1741 tggtccgacg tcgagaatga agccaagacg ggcacgacga gcttgatcac actggcctta
     1801 ctgacggccg gcgagagtcc agagtcgcag gccattcgca aggcactgga atacctgcgc
     1861 ggtttcggcc ccaacgatct gcacagcacc tacgctgtct cgcttcaaac catggttttc
     1921 gcagcggccg aacccgagcg tgatcagttg cggatcgcca acaatgtctc ctggttggaa
     1981 cgcgcccaga tcaagccggg cgatcctgta tactggccgg gttcgtggac ctattccgac
     2041 tcgaaacggg gccggcccgg agacaactca aacacccagt acgcgctcct ggggctgttc
     2101 gccgccagtg aggttggagt tcccgtcaaa ccgatggtct gggagctgtc gcgtctctac
     2161 tgggagaaga gccagaaacg cgacggcagt tggggttata ctcccgacgt gtcgaactcg
     2221 actgcgagca tgacctgcgc ggggatttcc agcttgatta ttacggggct ccgccgattc
     2281 cagggtcaag aattccttca gggcgactcg atcgaaaact gcggcaaagg aggaatgaat
     2341 cgtaatctcc aggccggaat cgattggctc gccaaccgct tccaggttgg gcagaatttc
     2401 ggccacggcc agcagtggaa atattattac ctttacggcc tggagcgggc cggtcggctg
     2461 gctggcgtcc ggttttttgg ccaaaacgat tggtatcggc tgggcgccga ggagctcgtc
     2521 cacgagcagc ataagctcgg agggttctgg atgggcgcgc tcaacgagtc ggaaaaggtc
     2581 ctggcgacca gttttgccat cttgttcctc gccaagggcc gcgcgccggt gctgatcaac
     2641 aaactgcgcc acgagcccgt gggcgactgg aacaacgacc ccgacgacgt ccgaaacatg
     2701 gtcgggattg tctcccgcga ctggaaaagc ctcctgacct ggcaagtggt cgagcccggg
     2761 atcgccaccg tgtccgagtt gcttcaggcc ccgatcgtct acttcaatgg ccaccacgcg
     2821 ccccagtttt cgcaactcgc ccgcaggaat ttgcgcgaat acgtcgagca aggaggtttt
     2881 attttcgccg aggcctgttg cggcaaacca gagtttgacc aaggattcaa gcagttgatg
     2941 aaggagatct ttcccgagga ggaatacaag ctcagaccgc tctcggagga tcatcctgtc
     3001 tggcgggccc gccacatgct tgcgccagat ctccaccctc tttggggaat cgagcacggt
     3061 tgccgtacag tcgtcatcta ttcacccgcg gacctgtcgt gttactggaa ccagagcgag
     3121 caaagtccgc tcaacacggc agtcatgaag tccatcaagg tcggccagaa cgtgattgac
     3181 tacgcgaccg gtcgagagat gccggcggac aagctcacaa tccgcgacgt tcgcgatttc
     3241 aagctggatg cacccaaacg aaacgcgctg cgcattgcca agctcaagca cgccggcgac
     3301 tggaacgtgg caccgcaggc catacccaat ctgatggatg ccctgcgcaa gccgccgttc
     3361 cgcttcgacg tggtcgttac tcaaaaagac ctctatcccc gcgaccccaa cttagtttat
     3421 taccccctcg tctacattca cggtcgagca gcgctgtcat tctcgaagga agacatggac
     3481 gctttgcacc agcatcttga tcctgggggc ggcacgcttt tcgccgatgc tgcctgcggc
     3541 agcccggcct tcgacgccgc atttcgccac tttgccgctg agatgttccc caataatccc
     3601 ctggtcccga taccacgcga tgaccagctc tttaccgtgc aggtcggcgc cgacctctcg
     3661 gacgtccagt acaccaaggg agccggcggt ggccgcgatt ttccgcagct cgagggagtt
     3721 aagatcaacg accactgggt catcatctat tccaagtacg acatcggctg cgctctcgaa
     3781 cgacataccg gcattgagtg taagggctat acctatgaaa gtgcgctcaa gatcgccggc
     3841 aacatcgtga tctactcgac tctgccgtga aaaaaggggc gggacccttt cataaaggga
     3901 tcccgagcct ttttgctctg caccgcggcg aatcgcggca agcaaaggca tggccgcacc
     3961 gaaaaccgac gagggtcatg ccctgacccc aaacgtgcgc cgtgccagca gaga
//
LOCUS       DATAQL010000258         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_26230, whole genome shotgun sequence.
ACCESSION   DATAQL010000258 DATAQL010000000
VERSION     DATAQL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26230"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VHS97_04395"
     CDS             complement(<1..323)
                     /locus_tag="VHS97_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3447465.1"
                     /translation="MEVSTSAKFPHAEFFPPAPAPRGSSRWLARMVDDRREIGRFWPV
                     IQNMVVQDLRVKYQRSILGFLWTLLNPLLMMATLSWVFSYLMKGFDNFPLFLFAGMVP
                     WSFLSG"
     gene            complement(500..2368)
                     /locus_tag="VHS97_04400"
     CDS             complement(500..2368)
                     /locus_tag="VHS97_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3447466.1"
                     /translation="MSTKLNRRDLLVAGGAVGATAAVSALVPKADAGVELGRYARRVG
                     VQGKMTGAQATAAALACQGVRCVFGIPGAQTNELWDALKARSIPYLLVANESSAAVMA
                     DASARATGEVGVFSVVPGPGLTNALTGIGEAFYDSIPIVGIVSDIDRSPKAPIGQVHG
                     LANAAILRPVVKGLIEVRHQAEIPGALFQAYQAANMGEPGPVAVVIPFPFYTEVWDYD
                     MPAPPPCPLPFDEAAYGRVLAHLMNRRQRVGIYAGLGCVDTGTSLASVAELLQAPVAT
                     SVSGKGCIADAHPLAVGWGYGKQGTRTAEAAFKDVDLVLAIGVRYSEVSTASYAIPRH
                     EVLIHVDANPQNLGRNVPAHVTLCADARLFLDRLVADGAAIARPACPELWQKIHRLRQ
                     DDRCQAATVKITPCVDPMFFLSQFRCALGPDELFFVDVTAATHWASEAIEVQGPRRYF
                     TPADNQSMGWAIPAAIGAQRVRPDRQVVCVTGDGCFLMSAIEMSTAARAGLPVKFFVL
                     DDGGYHYMQMLQEPVYRRTTATEIARIDYAAFAQAVGLGYNQIVDNADALAGIQRALA
                     SPGPVLTRVVIRYDGREIRWLSAIRAQYLKKMPNGDKVRLAARIGVRALTPRADNY"
     gene            complement(2609..>3393)
                     /locus_tag="VHS97_04405"
     CDS             complement(2609..>3393)
                     /locus_tag="VHS97_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3447467.1"
                     /translation="FDDELSLAIKIGASLLEILPHWGRLLDAAKAREQAGDRGLLIHS
                     AHGCWGGRTIRARRVDLGSVDASTHGESIDDLKACIDWLAAAGGKYLVVHPGGLSDPE
                     EWTGRRMALSRGLLQLADHARGTDVVVCVENMPPGVYPGSRMAELAALLRELDRSNLA
                     LALDTGHANLTASPAEETRAAGSLLATTHVHDNNGRQDSHEPPGKGTIDWPDWGLALD
                     SISYRGPILLECIRHLRQNPASLKPEVVKGPLRTYRPIQDRP"
BASE COUNT          681 a         1109 c         1014 g          589 t
ORIGIN      
        1 ccgctcaaaa aactccaggg gaccatgccc gcaaaaagaa aaagtgggaa gttgtcaaag
       61 cccttcatca ggtacgagaa cacccaggac aaagtcgcca tcatcaagag aggattgagc
      121 agggtccaga ggaagcccag aatcgaccgc tggtacttga cgcgcaggtc ttgcacaacc
      181 atgttctgaa tgaccggcca gaatcggccg atctcacggc gatcgtcgac cattctcgcc
      241 agccagcgac tcgatccccg cggagcaggc gcaggcggaa aaaactccgc gtgcgggaac
      301 ttggcgcttg tacttacttc catgcgtgca acaaacccca tccatggcgt cgaacccgcg
      361 tttgccccga tccccgtaca atcgcgaggc atattacgga gaaggctcga atcgcggaag
      421 atcaaccgcg tgatctggaa aactttgaat tcttctaact cccccaaatt cacaggctgc
      481 accgaaaatt tgcaccgggt cagtaattat cggctcgagg agtgagcgcc ctcacaccga
      541 tccgtgcggc caggcgtact ttgtctccat tgggcatctt cttgagatat tgcgcgcgga
      601 ttgcgctgag ccagcggatc tcacgtccgt cgtagcggat cacgacgcga gtgaggacgg
      661 ggcctggaga cgcgagggcg cgctggatcc cggcgagcgc gtcagcgttg tcgacaatct
      721 ggttgtagcc gaggccaact gcctgggcga aagccgcgta gtcgatcctg gcgatctcgg
      781 tcgcggtcgt ccgacgatag acgggctctt ggagcatttg catatagtgg tatccgccgt
      841 cgtcgagaac aaaaaacttg accggcaggc cggcccgagc ggctgtcgac atctcgatgg
      901 ccgacatcaa aaaacaacca tcaccggtca cgcaaacgac ctggcgatcg gggcgaaccc
      961 gctgggcacc gatggccgcc ggaatcgccc accccatgct ttggttgtcg gcgggtgtaa
     1021 aatagcgtcg cggcccttga acttcgatcg cctcggatgc ccagtgcgtg gctgcggtca
     1081 catcgacaaa aaagagctca tcgggaccca gggcacaccg aaactggctc aagaaaaaca
     1141 tcgggtcgac gcacggtgtg atcttcaccg tcgccgcctg gcaacgatcg tcctgacgca
     1201 gtcggtgaat cttctgccat agctctggac atgccgggcg tgcgatcgct gctccatcgg
     1261 ccaccaggcg atcaaggaac aaccgcgcgt cggcgcacaa ggtcacgtgg gccggcacgt
     1321 tgcgtcccag attttgagga ttggcgtcga cgtggatcaa gacctcatga cgaggaatag
     1381 cgtacgacgc cgtcgagacc tcgctatacc ggaccccgat ggccagcacc aggtcaacat
     1441 ccttgaaggc tgcctcggca gtccgggtcc cttgcttgcc gtaaccccac ccgacggcaa
     1501 gcgggtgggc atcggcaatg cagcccttgc cgctaactga cgtcgccacc ggggcctgga
     1561 gcagctcggc gaccgaggcg agggacgttc ccgtgtcgac gcatcccagc cccgcgtata
     1621 ttcccactcg ctgccgccga ttcatcaaat gcgccaagac ccggccgtag gccgcctcgt
     1681 caaacggcaa agggcaggga gggggcgcgg gcatatcgta gtcccagacc tcggtgtaaa
     1741 acggaaacgg aatcactacg gccaccggcc ccggctctcc catattggcc gcctggtagg
     1801 cttgaaaaag tgcgccgggg atttccgcct ggtgccgcac ctcgatcagc cccttgacga
     1861 ccggccggag gatcgccgca ttggccagcc cgtgcacctg gccgatcgga gcctttggtg
     1921 accggtcgat gtcactgacg atcccgacga tgggaatact gtcgtaaaag gcctcgccga
     1981 tccccgtcag cgcgttggtc aatcccggac ctggcacgac cgagaagacg ccgacctcgc
     2041 ctgtggcccg cgccgacgcg tcggccatca ctgcggccga agattcattc gcgaccagta
     2101 agtatggaat cgaacgggct ttgagagcgt cccacaactc gttggtctgc gcccccggaa
     2161 ttccaaaaac gcagcgcact ccttgacagg ccagcgccgc cgccgtcgcc tgtgcaccgg
     2221 tcatcttgcc ctggacgccc accctccgtg cgtaccggcc cagctccact ccggcgtcgg
     2281 ccttcggtac gagcgccgag acggccgcag tagccccaac cgcgccacca gcaacgagca
     2341 gatcacgccg gttcagcttg gttgacacgt tcggggctcc ttcccggcca aattcatggc
     2401 acggcctcct gtggccgcgt ctcgcattcc cccggcacga ctcgtcacgt tgttgccggc
     2461 tctgcgcctg tttcgtcccg ggccctggca agtcacgatg gcgcatctac aaacgcttgt
     2521 atcggcatcc gggagtcagc ttcatcgtgc gaccattgcc ggagccggtg atcgcgagca
     2581 gcttcgccct catcttccgc aaaaccggtc aaggtcggtc ctggatcggg cgataggtgc
     2641 gcaagggccc ttttacgact tcaggcttca agcttgcggg gttttgacgg aggtggcgaa
     2701 tgcactccag caggatcggg ccgcggtagc tgatcgagtc aagcgcaagc ccccagtcag
     2761 gccagtcgat cgttcccttg cccgggggct cgtgcgaatc ctggcggcca ttattgtcgt
     2821 ggacatgggt tgtcgccagc aacgagcccg cggcccgcgt ctcctccgcg gggctcgcgg
     2881 taaggttggc atgcccagta tcaagcgcca gcgccaggtt ggagcggtcg agctcccgga
     2941 gaagcgcggc aagttcggcc atgcggctgc cggggtagac gccaggcggc atgttttcga
     3001 cacaaacaac gacgtccgtc ccgcgggcgt gatcggccag ctgcagcagg ccgcgcgaca
     3061 gagccatgcg ccgcccggtc cattcttcgg gatcagacaa gccgccagga tgcaccacca
     3121 agtacttgcc gcccgcagcg gccagccaat cgatgcacgc cttcaagtcg tcgatggact
     3181 caccgtgagt agacgcatct accgatccta agtcgacccg gcgggcacgg atcgttcgcc
     3241 cgccccagca gccgtgggcg ctgtgaatca atagaccgcg atcgccggct tgctcgcgcg
     3301 ccttggccgc gtcgagcagc cggccccaat gaggcaaaat ctccaataac gaggcaccaa
     3361 tcttaatggc aagactgagc tcgtcgtcga act
//
LOCUS       DATAQL010000259         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2926092, whole genome shotgun sequence.
ACCESSION   DATAQL010000259 DATAQL010000000
VERSION     DATAQL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2926092"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..580)
                     /locus_tag="VHS97_04410"
     CDS             complement(134..580)
                     /locus_tag="VHS97_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447468.1"
                     /translation="MRRPTPERHDEDDAFIAELVSEAGDPKVEPRPEHVAELRVLILD
                     RLGLARPRRRPRAWWLAVPGLAAACLAAVVIWPRGNTEDRPVSGSGRPSANPIASRPL
                     PDQGGIAGWRNIRQTLDVSEMPPFSWPLEGSSHLAVSTQIPADLLD"
     gene            complement(552..1139)
                     /locus_tag="VHS97_04415"
     CDS             complement(552..1139)
                     /locus_tag="VHS97_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447469.1"
                     /translation="MDDESRLVRAMARRDRSAWDAMYDRHVGDVFAVIYHLVGGNRQT
                     AEDLNQEVWLLAIEQFDRFDDKRGDFRDWLLGIARHRVFRHYRRATVQTVEGRPDPLS
                     DDLPPPELLEGRERAEVVRAALVCLHDGSRNMLLAKYAEGLSVAEIAAQSGRSAKAVE
                     SLLSRARRQLRILLEPYFSHTTGGQRHAATDARTT"
     gene            1930..3216
                     /locus_tag="VHS97_04420"
     CDS             1930..3216
                     /locus_tag="VHS97_04420"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX3447470.1"
                     /translation="MGSTCVVGLQWGDEAKGKIVDLLCDQHDVVVRYQGGANAGHTVV
                     VDGVTYKLSLVPTGILRPGVDAVIGNGVVIHPPALLREIETLAAQGIDVGRRLHVSDR
                     AHVILPYHIEEERLTEESASEADHLGTTRRGIGPCYRDKVGRVHGVRIADLYHPQAFR
                     DRLARVIDYKNRLLGAMLADYKPLDARSVADLYLEHAQRLRPFVRDTTTWLHEAIEGG
                     RRLLFEGAQGSLLDIDHGSYPYVTSSNSSAAGIAAGSGVPTRHIDRWIGIAKAYTTRV
                     GGGPFPTEQNNAIGERIRRIGNEYGTVTGRPRRCGWFDAVAVRYAARISGSTELAVML
                     LDVLSGIDALKVAVAYDFNGKRTNVLPSSLTDFEEYNPVYETLPGWDEDLTQIKKWSD
                     LPARARDYVEFLGRQVGVPVTIVSVGPERRQTILVP"
     gene            3309..>3811
                     /gene="uppS"
                     /locus_tag="VHS97_04425"
     CDS             3309..>3811
                     /gene="uppS"
                     /locus_tag="VHS97_04425"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX3447471.1"
                     /translation="MAITEAGLARIEAAGLKPEQLPRHIAIIMDGNGRWAQRRGLPRI
                     VGHRRGIQSVRAVVEEGCRLGLDQLTLYCLSVENWKRPPRELMFLMRLLRHFLVVERD
                     ELIEQNVQLKMIGRREGLPEDVLREFKRTAELTASNDGMILCLAVNYGGRSEIGDAAR
                     RLAEDVRA"
BASE COUNT          757 a         1120 c         1215 g          719 t
ORIGIN      
        1 tcggccctgg cgtgccccga gtgcagggca agaattgccg ttgaaaaaca cagagcgcgc
       61 agcatttacg atacctcact ttgagaaggt tgagacgaaa aggaacacgc ggacttcgtt
      121 cgggttcgct gctctagtcg agcaagtcgg caggaatctg ggtcgacacc gcgaggtgcg
      181 atgatccctc gagcggccat gagaatgggg gcatttccga cacatcgagt gtctgccgga
      241 tgttccgcca gccggcgata ccgccctggt ccggcagtgg tcgggaagcg atcggattcg
      301 ctgacggccg acctgaaccg ctgacgggcc gatcctcggt attgccgcgg ggccaaatga
      361 ccacagcagc caggcacgcg gctgccaggc ccgggaccgc cagccaccat gctcttgggc
      421 gccgcctggg acgggccagc ccgagccggt cgagaatcag cacacgcagt tcggcaacgt
      481 gctcgggtcg aggctcgacc ttcgggtcgc cggcttcgga gacgagttcc gcgatgaacg
      541 cgtcgtcctc gtcatgtcgt tcgggcgtcg gtcgccgcat ggcgttgacc tccggtcgtg
      601 tgagaaaaat aaggctcaag caggatccgg agctgacggc gagctcgcga gagcagcgac
      661 tccaccgcct tggcggatcg cccgctttgc gcagcgatct cggcgaccga gagtccttcg
      721 gcatacttgg ccaggagcat gttccggctc ccgtcgtgca agcagacgag ggcagcgcgg
      781 acgacttcgg cccgctctcg tccttccagc agttccggcg gcggcaagtc gtcggacagc
      841 ggatcgggcc ggccttcgac cgtctgaact gtggcgcggc ggtagtgcct gaaaacgcgg
      901 tggcgggcga tgcccagcag ccaatcccgg aagtcgcccc tcttgtcgtc gaatcggtcg
      961 aactgctcga tggccagaag ccagacctcc tggttgagat cctcggcagt ctgacgattc
     1021 ccgccgacaa ggtggtagat caccgcgaag acgtcgccga cgtggcgatc atacatcgcg
     1081 tcccaggccg aacgatcgcg ccgtgccatt gctcgaacca ggcgcgattc gtcatccatc
     1141 ggccgctggc ctcgataaag ttgatgttcc tccactcatt ctattgccgt gagccggcag
     1201 aatccttcgc cacttgtcga gaattcacga tcgcgtcatc tgcagattga agtcaccgag
     1261 cggcctcgca ccgagattcg caatttgcgc tttcactgaa aactgtgcga agcacatgta
     1321 aaacgaacgt ggactacgat tccaaaaatt gcttggcggt cgacgcttca cttctggttg
     1381 aaggtcggtt attgagagca tgcggaggtt cttcgaccgc tcagtaaagc acgttcatga
     1441 tcctagtgct tcaatcgcaa ctccgggtaa ccccgagatg agtgaaattc gttaagaggc
     1501 cttagcactg cagttctgag gaatgggccg gtacctgacg agccggattg ccgacttgtc
     1561 agccaacggt cccgtcctca ggaaaacgct catcatcgac caatcccgag gtcggatggc
     1621 tctccatcat aagaagagat tcgcctttcg gggaaaggca ggaaaacccg gcaaagcagc
     1681 aatctcgttc gatattcagc gactctcggg cgttctcgga aagtgggaaa gaacctacgg
     1741 aatttccttc tctcaaaggg cggcgcgccg taagtccttg tcgcactgca tccgaaaaga
     1801 tgaaacgcct cacaaggggg gagggcgaca gaaaggatcg ttgacgcctt caaccgggag
     1861 cggtaccttt aaactttacc gcaccaagtg accttcgtgg tcgacttgat caattggaga
     1921 gggtttgctg tgggttcgac gtgtgtggtg gggctgcagt ggggcgatga ggcgaagggc
     1981 aagatcgtcg atttgctttg cgaccagcac gacgtggtcg tgcgctatca ggggggtgcc
     2041 aacgccgggc ataccgtcgt cgtcgacgga gtgacctaca agctttccct ggtgccgacc
     2101 ggcatcctcc ggcctggtgt ggacgcggtc attggcaatg gcgtggtgat ccacccgcct
     2161 gccctgctcc gggagatcga gacgttggcg gcgcagggaa tcgatgtcgg ccgccggctt
     2221 catgtcagcg accgcgccca tgtgatcctt ccctatcaca ttgaggagga acgacttacg
     2281 gaggagtcgg cctccgaggc agatcacctg ggaacgacgc gccgagggat tggcccctgc
     2341 tatcgcgaca aggtcgggcg agtgcacgga gtgagaattg ccgaccttta tcatccccaa
     2401 gcatttcgcg atcggctggc acgagtcatc gattacaaga accggctctt aggcgccatg
     2461 ctggcggact ataagccact ggacgcacgc tcggtggctg acctatacct ggaacacgcc
     2521 cagcggctgc gtccgtttgt tcgcgacaca acgacatggc tgcacgaggc gatcgaaggg
     2581 ggcaggcggc tcctttttga aggagcccag ggttccctgc tggatatcga tcacggcagc
     2641 tacccgtatg tgacgagctc taacagcagc gccgccggaa ttgccgcggg gtcgggcgtc
     2701 cccacgcggc acatcgaccg ctggattggt atcgccaaag cctacacgac ccgggtgggt
     2761 ggcggaccct ttcccaccga gcaaaacaac gcgatcggcg agcgcatcag gcgcatcggc
     2821 aacgagtacg gaacggtcac gggccggccg cggcggtgcg gatggttcga cgccgtcgcg
     2881 gtgcgctatg cggccaggat cagcgggtca accgaactgg cagtcatgct tctggacgtt
     2941 ctgagcggca tcgatgcgct caaagtcgcc gtagcctatg atttcaacgg aaaaaggaca
     3001 aacgtcctcc ccagctccct caccgacttc gaagagtaca atccggtcta cgaaacgctg
     3061 ccggggtggg acgaagatct cacccagatc aagaaatggt cagaccttcc cgctcgcgca
     3121 agggactacg tcgagttcct gggccggcaa gtcggggtgc cagtgaccat cgtctcggtt
     3181 ggccccgagc ggcgacagac catactcgtg ccctgacggc tctcctcggt cggatgatcg
     3241 cgggctgatg tttgacttga tcgttctttc gttctagctt acttgaggct tgtcgattga
     3301 gaccagacat ggcgatcacc gaagcagggc tggcgcggat cgaggcggcc gggttgaaac
     3361 ccgagcagct cccgaggcac atcgcgatca tcatggacgg caacgggcgc tgggcccagc
     3421 gtcgtgggct gccgcgaatc gtgggccatc gacgggggat acagagcgtc cgcgcggtcg
     3481 tcgaagaggg atgccggctg gggctggacc agttgacgct ctattgtctg tcggtcgaaa
     3541 actggaagcg gccaccgcgc gagttgatgt tcttgatgcg gctcctgcgg cacttcctgg
     3601 tcgtcgaacg cgatgagctg attgaacaga acgtccagct caagatgatt ggccgccgcg
     3661 aaggtctgcc cgaggacgtt ctgcgcgagt tcaaacgcac tgccgagctg acggcttcga
     3721 acgacggcat gatcttgtgc ctggctgtga attacggcgg gcggagcgag atcggtgacg
     3781 ctgcacggcg gctggccgaa gacgttcgcg c
//
LOCUS       DATAQL010000260         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4453911, whole genome shotgun sequence.
ACCESSION   DATAQL010000260 DATAQL010000000
VERSION     DATAQL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4453911"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1293
                     /locus_tag="VHS97_04430"
     CDS             385..1293
                     /locus_tag="VHS97_04430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3447472.1"
                     /translation="MTPPVNRRDFMAQAAVTATAAVTMTTAVSHGRESRGRTALPKSA
                     TDMVTLGKSGIQVSLIGMGTGSVGSGQASNQTRLGVKEFTRVVRYALDHGIHFFDVAD
                     QYGSHVYLREALRGVPRDQYVIQTKTHATKLADARSHLERYRLELGVEYIDIVLLHCM
                     QKTAWPHDHEGSMEYLMKAKDEKIIRAHGTSCHGMDPLRTSAKNPFVEIDLARVNPEG
                     LIMDDKKPDEVASVLEEMHNAGKGIIGMKILGEGRINTPERKDASLRYVLGLGTVDSF
                     IIGFQSTDQVDDILKRTGDAITYLKS"
     gene            complement(1458..1643)
                     /locus_tag="VHS97_04435"
     CDS             complement(1458..1643)
                     /locus_tag="VHS97_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447473.1"
                     /translation="MRQVGDTLVWLAMITVVVAVVYFTPRLANYVALFGADHGRGCVT
                     CHEIAYQPEETTPVQTH"
     gene            2133..2609
                     /locus_tag="VHS97_04440"
     CDS             2133..2609
                     /locus_tag="VHS97_04440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3447474.1"
                     /translation="MAIQKLLRVGDWAPDFTLAGSNGELVSLSDFRGRSEVVLFFYPK
                     DHSPACTAEACSFRDSYAVFHERGAEVIGISADSARSHKGFAARFRLPFILLSDSDGS
                     VRERYGVAKTLGIFPGRATYLIDRQGVIRHIFSSQFMPWRHVSSVLGVLETLRDEQ"
     gene            2813..>3969
                     /locus_tag="VHS97_04445"
     CDS             2813..>3969
                     /locus_tag="VHS97_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3447475.1"
                     /translation="MNRSTLAGNLAGFARFLMVLSGFASPSHGGEVKLDGRTFTIPDG
                     FSIERIAGPPLVDRPITAAFDDLGRLYVADSSGSNDNVKKQLAENPHRIVRLEDRDGD
                     GKFDKQTVFADKMMFPEGTMWYGDSLYVAAPPSIWKLTDTNGDGLADERVEWFQGKTL
                     TGCANDLHGPYRGPDGWIYWCKGAFAPQTYERPGKSPFATRASHIFRCRPDGTGIEPV
                     MTGGMDNPVDVAFTSGGERIFTTTFFVHPGGGQRDGLIHAIYGGIYGKIHEPIFEPAH
                     KWTSPHVMPVLLHMGPAAPCGLTRYESDGFGTAYQNNLFACYFNLHKVSRHVLVPQGA
                     SFTTKDEDFVSSPDLDFHPTDVIEDADGSLVIVDTGGWYKLCCPTSQLQKP"
BASE COUNT          875 a         1175 c         1107 g          812 t
ORIGIN      
        1 cattcgaaga attaggatga agattacgat tacgaagagg attacgagca cgatcaagat
       61 taagattagt acttaaaaat cacggcgacg agtagagtca ttctcttgct cgtactcttg
      121 gccctaatcc tcttcataat cataatcatc atcttactct tactcgtaca tgcacgccgc
      181 tggtcaacca gctagacaaa gactgacggc ccaccagggc tgctctatag cagcaacgaa
      241 tggagcgggt tagaatcacg aatcgacgcc gcgcacgccc acggccgcgc gatccagagc
      301 aaatcgatac gatgcgggcg ttcatctggc tccaaaatcc atcaatgagt tgatcggaac
      361 ttttgtaccg aggagaacga tgtcatgact ccccccgtaa atcgccgcga tttcatggcc
      421 caggccgccg ttacggcgac cgccgcagtc acaatgacaa ctgccgtctc ccatgggcga
      481 gagagccgcg gccgaaccgc cttacccaag agcgcgaccg acatggtgac gctgggcaag
      541 agcggcatcc aggtctctct gattggcatg ggtacaggga gcgtaggtag cggtcaggcc
      601 agcaatcaga cccgtttggg cgtcaaggag tttacccgcg ttgtccgcta cgccctcgat
      661 cacggaattc actttttcga cgtcgccgat cagtacggct cgcacgtgta tcttcgtgaa
      721 gccttgaggg gcgttccccg cgaccagtat gtgattcaaa ccaaaacaca tgcgaccaaa
      781 ctagccgatg cgcggagcca cctcgagcgg tatcgcctcg agctgggggt cgagtacatc
      841 gacatcgtgc tcttgcactg catgcagaaa accgcctggc cacatgatca tgaaggttcg
      901 atggaatacc tgatgaaggc caaggacgaa aagatcatcc gcgcccacgg cacgagctgc
      961 catggtatgg atcccttgcg cacgtcggcc aagaatcctt tcgtcgagat cgacctggct
     1021 cgcgtcaacc cggaaggact gatcatggac gacaaaaagc ccgacgaggt cgcttcggtt
     1081 ctggaggaaa tgcacaatgc cggcaagggg atcatcggca tgaagatctt gggggagggc
     1141 cggatcaata ctccagagcg aaaagacgcc tcgttgcgct acgtccttgg cctgggaact
     1201 gtcgactcct ttatcatcgg tttccagtca accgaccaag tcgacgatat cctcaagcgg
     1261 accggcgatg cgatcaccta cttgaagagt taacctacga aaatcgcagg ccgagtcgcg
     1321 gtgttgcatt ccgcccaaga tgcgccatcg cactcgacct ggatcgtccc gcaccacgaa
     1381 acggggcgat gcctgaagtc aatatccggg ctcgacgtca atagtacgcc cattccgtga
     1441 gcgtcacccg gggtatctta gtgggtctga acgggagtgg tctcttccgg ttggtaggcg
     1501 atttcgtgac atgtcacgca gccgcgtccg tgatcagcgc cgaacagagc gacataattc
     1561 gccagtcggg gagtaaaata gacgacagca accaccaccg tgatcatcgc caaccagacc
     1621 agcgtgtctc caacctgtcg catgacagac cgcctcctac gaattgaagc gtgaactaag
     1681 cgacgatggc tttgctccta ggagttggga gcaaaactgg cgcatccgtt cgctccgtca
     1741 ccttatgtgt catttcgcac gaccccaaaa ccccaacgta cctatcacaa aggacatttg
     1801 cgtgcgcgac cgcggtcgcg catcccactt gcgactacgt ttttgacccg atagctaagc
     1861 aaaaataggg tgtccagcga agccgcgaat ggacagcttg caaatcactc gcctaaccgc
     1921 tcaaattctt aaagccgagg ctcgttgagg ctgttaactg atgctctagt ggaacaatag
     1981 gtgattttct gaattctgtc ttctgcaaaa tcgtcctaca acatgaaccg actaccagcc
     2041 aggatgtgac tgctaagatt atattttgcc acaccccctg agcacttcgt gaccagccgc
     2101 gaacttgagc agctccctgc gtaggaaaca ccttggcaat acaaaagttg ctgcgagtcg
     2161 gcgattgggc gcccgatttt acgctggctg gatcgaacgg cgaactcgtg agcctgtccg
     2221 actttcgcgg ccgatctgag gttgtgttgt tcttctatcc caaggatcac tcgcccgcat
     2281 gtaccgccga agcctgctcg ttccgcgata gttacgcggt gttccacgaa agaggggccg
     2341 aagtgattgg catcagcgct gactccgccc gatcgcacaa agggtttgcg gcgcggtttc
     2401 gtcttccgtt tatactttta tccgacagtg atggttcggt gcgcgagcgc tacggtgtcg
     2461 ccaagacttt gggaatcttc cccggtcgcg ccacgtatct gatcgatcgg cagggcgtga
     2521 tccggcacat tttctcgtcg cagttcatgc cctggagaca cgtgtcgagt gtgctaggcg
     2581 tgttagaaac gctccgcgac gagcaataag gctgcttccg cgggttgccc cgcgcgcgtc
     2641 gccgcccgat tgacgccaga gcgaaccctc gcatagaaca tcttcggtag attgcggaac
     2701 agcttgatgg cttacggcat ctgaccggcc cggccagccg acctttcgtc ggccacgcgg
     2761 ttgttgcccg cgccgcggaa ccaagatcgg cacagtccgg agactcgttc tcatgaatcg
     2821 atcgaccctg gcggggaatc ttgccggctt tgcccggttc ctcatggtct tgtctggatt
     2881 cgcgagccca agtcacgggg gcgaggtcaa gttggacggc cggacattca cgattccgga
     2941 cggcttttca atcgaacgga tcgccggacc acccctggtg gatagaccga tcacggccgc
     3001 gttcgacgat ctggggcggc tctacgtcgc cgattcctcc ggctccaacg acaacgtcaa
     3061 aaagcagctc gcggagaatc cgcatcgcat tgtccggctc gaagacaggg acggcgatgg
     3121 aaaattcgac aaacagacgg tctttgccga caagatgatg ttcccggagg gaacgatgtg
     3181 gtacggcgac tcgctctacg tcgctgcgcc gcccagcatc tggaaactga cggacaccaa
     3241 cggcgatggt ctggcagacg agcgggttga gtggttccag gggaagactt tgaccggttg
     3301 tgcgaatgat ctgcacggac cctatcgcgg gcccgacggc tggatttact ggtgcaaggg
     3361 cgcatttgcg ccgcagacgt acgagcggcc gggcaagtcg ccgttcgcca cgcgcgcgtc
     3421 acatatcttc cgctgccggc ccgatggaac cggtatcgaa cccgtcatga ccggcggcat
     3481 ggataacccg gtcgacgtcg cgtttacttc cggcggcgag cgcatcttca cgacgacctt
     3541 ctttgtgcac cctggcggcg gtcagcgcga cggcctgatc catgcgattt acggcggcat
     3601 ttacggcaaa atccacgaac cgatcttcga gccggcgcat aagtggacaa gcccgcatgt
     3661 catgccggtc ctcctgcaca tgggaccggc cgctccgtgt ggcttgacgc gttacgagtc
     3721 ggatggcttc ggcacggcgt atcaaaacaa tctgtttgcc tgctatttca acctgcacaa
     3781 ggtgagccgc cacgtattgg tgccccaagg cgccagcttc acgaccaaag acgaagattt
     3841 cgtctccagt cccgatctcg acttccaccc taccgacgtg atcgaggacg ccgatggcag
     3901 cctcgtgatc gtcgataccg gcggctggta caagctctgc tgcccgacgt cacagcttca
     3961 aaagcctga
//
LOCUS       DATAQL010000261         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2621919, whole genome shotgun sequence.
ACCESSION   DATAQL010000261 DATAQL010000000
VERSION     DATAQL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2621919"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VHS97_04450"
     CDS             complement(<1..390)
                     /locus_tag="VHS97_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3447476.1"
                     /translation="MNRRGFTLIELLVVIAIIAVLIALLLPAVQEAREAARRIQCTNN
                     MKQLGIAHHNYHDTFGSLAAARIFVSTACAGGNVRDGCQDTPWFCLMLPQFEQQTMYS
                     AFNFAVGESGPLVGSRLGLGNFVNSTVF"
     gene            898..2601
                     /gene="ggt"
                     /locus_tag="VHS97_04455"
     CDS             898..2601
                     /gene="ggt"
                     /locus_tag="VHS97_04455"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3447477.1"
                     /translation="MRIRWLSAFLVVIGFTAVTGRVVAHDRPAGPLHKSRSCVVARNG
                     IAATSQPLATDAAIRILQAGGNAVDAAIAANAVLGLVEPMSCGIGGDLFAIVWDAKSK
                     KLYGLNASGRAPGAATIDFYQSKGFTEIPGSGPLSWSVPGCVDGWDQLRTRFGSKSWE
                     EVLAPAVAYAEEGFPVSELIAAAWNGAATRLAKVRSSAACYLPNGRAPKFAEVFQNHG
                     LARSYRTIVSEGRDGFYKGSLAEAIVAYSKSVGGLFEQKDFADHTSTWVDPVTTNYGG
                     YDIWELPPNGQGIAVLQILNLLEPYDLKAMGPQSAEALHLMIEAKKLAYEDRAKFYAD
                     PEFAKVPVAELISKVYAERRGKLIDPARSNARPTHGDPKPADTIYMTVADKAGNAVSL
                     IQSNFNSFGSSHVAGELGFVLQNRGSSFALDPSHANRLEPHKRPFHTIIPGFVTKDGK
                     AWLSFGLMGGDMQAQGHAQVLVNIIDFGMDVQAACDAPRFYHSGSSRPTGAVAKDGGS
                     VALESEIGPEVRKALEAKGHRIVNERASYGAYQAIRIDADNGVLIGGSESRHDGQAIG
                     Y"
     gene            complement(2688..3029)
                     /locus_tag="VHS97_04460"
     CDS             complement(2688..3029)
                     /locus_tag="VHS97_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX3447478.1"
                     /translation="MRCPRAPALQGAGYCRTDLPLLDRSLITDYLYVTDAEAVAAARH
                     LAAREGILGGFTTGAHLAAAWKLLSERESGATIVFLVCDSGLKYMSTDLYLGVRPQAE
                     SAALSAEDRGR"
BASE COUNT          645 a          968 c          978 g          579 t
ORIGIN      
        1 aaagacagtg ctgttcacga agtttcccag cccaagcctg ctccccacga gcggaccgga
       61 ctcgccgacc gcgaagttga acgcgctata cattgtttgc tgctcgaact gtggcagcat
      121 gagacaaaac cagggcgtgt cctgacatcc gtctcggacg tttccgcccg cgcacgcggt
      181 ggagacgaaa atccgcgcgg ccgcgaggct gccgaacgta tcgtgataat tgtggtgcgc
      241 gatcccgagt tgcttcatgt tgttcgtgca ctggattcgc cttgccgctt cgcgggcctc
      301 ctggacggcc ggcagcagca atgcgataag gactgcaata atcgcgatga ccacgagaag
      361 ctcgatcaag gtgaaacctc gccgattcat cgggtgacgt cctttccgta cctcacgatc
      421 cgaacggtcg cgaccggttc gctaatcggg cgggagatga cactcttccc agataacccg
      481 ctcaagccca atccgaatcc attgggctcg acgccgaaca cactaatgac caacgaaact
      541 ccaggcaagc gcgctcaacg tacatcagac cgaattcacg ttaacttaaa gcaatgttct
      601 tacgatgtga ggtgcttgcc agcatgctgt gattccgccg cgccgctctc taggccgaca
      661 ctgtgccggc ggtcgctacc gccgcgacac cgaaccgcaa ggccttgctt tcatactact
      721 gggctgctcg aatgggggcg cactgacctc aatcgtgggt aaacatacaa agtctacagt
      781 tggtgggtgc ccgaaagctt cagtggacag cgaccatcgg agaggcgcgg tccgcccgac
      841 gaagttcgct tgatcgcgtg cgcctctaga ggggattgca tcatgacgat tcgacacatg
      901 cgtattcgat ggctgtccgc atttctggtc gtcatcggct ttacagcggt gaccggtcga
      961 gtggtggctc acgatcgacc agcggggcca ctgcacaaga gccggtcgtg cgtagttgcc
     1021 aggaacggca ttgccgccac cagccaaccc ctcgcgacgg acgcggcgat ccggattctg
     1081 caagcaggcg gaaatgccgt cgacgcggcc atcgcagcca acgccgtatt gggcttggtt
     1141 gagcccatgt cgtgcggaat cgggggagat ctgttcgcga tcgtctggga cgcgaaatcg
     1201 aagaaacttt atggcttgaa tgcgagcggc cgtgcccccg gtgcggcgac gatcgatttt
     1261 taccaatcca agggatttac cgagatcccc ggaagtgggc ccctgagctg gtctgttcct
     1321 ggctgcgtcg atggatggga ccagttgcgc actcggtttg gctcgaagtc atgggaggag
     1381 gtgctcgctc cggcggtcgc atatgcagaa gaggggtttc cggtcagcga actcatcgcc
     1441 gcggcctgga atggggctgc aacgcgactg gcaaaggtac gatcgtcagc cgcgtgttac
     1501 ctgccaaatg gccgggcgcc gaagttcgcc gaagtcttcc aaaatcacgg gctcgcacgg
     1561 tcatatcgaa ccattgtcag tgaaggtcgc gacggctttt acaaagggtc ccttgccgaa
     1621 gcgatcgtcg catactcgaa gtcggtggga gggctgtttg aacagaagga ttttgcggac
     1681 cacacgagca cttgggtcga tccagtaacg accaactatg gtgggtacga catctgggaa
     1741 ctcccgccta acggccaggg aatcgccgtc ctgcaaatcc tcaacctact cgagccgtac
     1801 gacctcaaag ccatggggcc gcagtcggcc gaggcgctcc atttgatgat cgaggccaag
     1861 aagctcgctt acgaagaccg ggccaaattc tacgccgacc cggaatttgc caaggttcct
     1921 gtcgctgagc tgatctccaa ggtatatgcc gagcgccgag gaaagctgat tgaccccgcc
     1981 cgctccaatg ctcggcccac ccacggtgac ccgaaaccgg ccgacacaat ttacatgacc
     2041 gtggccgaca aggctggcaa cgcagtcagt ctcattcaga gcaatttcaa tagcttcggg
     2101 tcgtcgcatg tcgccggaga actcgggttc gtgcttcaaa accgaggcag ctcgttcgcg
     2161 ctcgatccct cgcacgccaa ccgcctcgag cctcacaagc ggccgttcca cacgatcatt
     2221 ccaggcttcg tgaccaaaga cggcaaagcc tggctttcgt tcggactgat gggtggagac
     2281 atgcaagccc aggggcacgc ccaggttctc gtcaacataa tcgatttcgg gatggacgtt
     2341 caggcggcct gtgacgcacc ccgcttttac cattccggct cgtctagacc taccggggcc
     2401 gtggcgaagg acgggggctc ggtcgcgctc gaatccgaga tcggcccgga ggttcgcaag
     2461 gcactcgaag ccaagggcca caggatcgtg aacgaaaggg cgagctacgg cgcctatcag
     2521 gcgatccgca tcgacgccga caatggcgtc ttaatcggag gctcggaatc gcggcacgat
     2581 ggccaggcga tcggctattg accggggatc tacggcagag aagaggcggg gcacgcagac
     2641 tactccagtt cgggtgatgg ccgggtgccg ggcgaccgcg ggagtgctca ccgcccccga
     2701 tcctcggcgc ttagagccgc gctttcggct tgaggccgga cgcccaggta aaggtcggtg
     2761 ctcatgtact tcaacccgct gtcgcagaca aggaacacga tggtcgcgcc cgactcccgt
     2821 tccgagagca gtttccaggc ggcggcgaga tgggcgccgg tggtgaagcc gccgaggata
     2881 ccttctcgag cggccaggtg gcgcgctgcg gcgacagcct cggcgtccgt gacgtaaagg
     2941 taatcggtga tgagcgagcg gtcgagcagc gggaggtcag tccggcaata tccggccccc
     3001 tgaagcgctg gtgcccgagg gcaccgcaga cgacggccaa ccccgccccc atgttgccgc
     3061 tcgtcagcgc gacgaccgtc tggcccgcca ccagcgaccc gtccgcccgg gcccggcgga
     3121 tgagttccag ggcggcacgg tctttcagat cagcgtgcaa gcgcatggcc
//
LOCUS       DATAQL010000262         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1247696, whole genome shotgun sequence.
ACCESSION   DATAQL010000262 DATAQL010000000
VERSION     DATAQL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247696"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..>1957
                     /locus_tag="VHS97_04465"
     CDS             178..>1957
                     /locus_tag="VHS97_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX3447479.1"
                     /translation="MPTESTRQPRQLVTMAFILVAMATLATELTVAAPGQAILPRAAA
                     SLALIGWTFVIYSYLKARATRESNPSLLTMVIASLMIVALWGFDITRAIATTERLAPE
                     LIMVVLLRAATLTFAALVGNAVCLRISGGLTVALMLFASVLTEQRAIYGLTFAYALLG
                     SYWLILLYWSQIQVKLLEGRSKRPPILAIAVWGLITGLLIVLVVGPRQTIGVLAEWVG
                     TSGGTTIASPDARGGVGDGQDLAAGTEHAQSTGPVESDIFLETKEHSLYDASNEKWGE
                     PDKPKKNDQSLAVAVANEPSVIRQEKSKQPNPAREFSVIRKRRRDQSTPPTIEAKAAY
                     FVTGKTPLHIRLAAFDEFDERRWLEPASETESDGLRLGFGGWILLPTFQPSVVAGDLA
                     HKIVVGSVDCPQTPLPPYAHSFRIDLIDRPDFFRFSQPGILTYRINTGLPRQTVLESR
                     SHTIDPEALRRLRFPWGRIYALPKFLSLRGSAADSGEASRNDPASSLDPRILELASSW
                     AGGTERGWRQVEVVVRHLREEYRYDPEAELLSDYPDPIPYFLFEQRRGDDYAFATAAC
                     LMLRSLGYPCRFVEGLYAHPRRFDPKT"
BASE COUNT          366 a          628 c          580 g          383 t
ORIGIN      
        1 caagcgctgc gagctgatcg tttgcgagcg ccaggctagc gcgaacgtgc atctccaaat
       61 catcctcgac gccgatcacg ccgttcaccg aggaagcggg ccgtggagtt cgcgcgaatg
      121 ggcgatcagc cgatgacgtg ccggccactt tccggcgctc ctggagagag gtactccatg
      181 ccaactgaat cgactcgcca gccccgacag ctcgtgacga tggcgtttat cctcgtggca
      241 atggcgacct tggcgacgga gctgaccgtc gctgcacccg gacaagcaat tcttccacgt
      301 gcggcagcct cgcttgcgct gattggctgg actttcgtca tttactcgta cctgaaggcg
      361 cgagcgactc gagaatcgaa cccgtcgctc ttgaccatgg tcatcgcctc tctaatgatc
      421 gtcgcgctat gggggttcga catcacccgc gcgatcgcga cgacagagcg attggcgccg
      481 gagctcatca tggtcgtctt gctccgcgcc gcgacgctca cgttcgcagc cctggtggga
      541 aacgcggtct gtctacgcat ttcaggtggc ctgaccgtgg ccctcatgct cttcgcctca
      601 gtgctgaccg aacaacgtgc gatctacgga ctcacgtttg cttatgcact attgggcagc
      661 tactggctga tcttattgta ctggagccag atccaggtga agctcctcga gggaaggagc
      721 aaacggcccc cgattctggc gattgcggtc tgggggctaa tcaccggttt gttgatcgta
      781 cttgtcgtcg gaccccgcca gacgatcggt gttttggcgg aatgggtcgg cacgtcgggg
      841 ggcacgacca tcgcctcgcc cgacgctcgc ggcggcgtcg gcgacggtca ggacctcgcc
      901 gctggtacgg aacacgcgca gagcacagga ccagttgaga gcgatatctt cctggagacc
      961 aaagagcact cgctttacga tgcctccaac gagaaatggg gagagcccga caagcccaag
     1021 aaaaacgacc aatcgctggc ggtcgcggtc gcaaacgagc caagcgtaat ccggcaagaa
     1081 aagtcgaaac agccaaaccc ggcccgcgaa ttctctgtga ttcgcaagcg gcggcgcgac
     1141 cagtcgacgc ctcccacgat agaggccaaa gccgcttact tcgtgacggg gaaaacaccg
     1201 cttcacatcc gcctcgcggc cttcgatgaa ttcgacgagc ggcgctggct tgagccggcg
     1261 tccgaaacgg agtccgacgg tcttcgcctg gggttcggcg gctggattct cttgccgacc
     1321 ttccagccct cggtcgtggc gggcgacctg gcgcacaaaa ttgtcgtggg gagcgtcgat
     1381 tgtccccaga cccctcttcc accgtatgca catagtttcc ggattgacct gattgaccgc
     1441 cccgacttct tcaggttttc acagccggga attctcacct accggatcaa taccggcctg
     1501 ccgcgccaga ccgtcctgga gtcccgctcg catacgattg accccgaagc tctgcgcagg
     1561 ctgcgattcc cttggggccg catatacgca ctaccgaagt tcctgagcct gcgcggttcc
     1621 gcggcggact caggcgaagc gtcacgcaat gaccccgcct catcgctcga tccgcggatc
     1681 ctcgagcttg cgtcatcctg ggccgggggc acagagcggg gctggcggca ggttgaggtc
     1741 gtcgtcaggc acctgcgtga ggagtaccgt tacgatcctg aagccgaact actctctgat
     1801 tacccggacc caatacccta cttccttttc gagcaacggc gcggtgacga ttatgcattt
     1861 gccacggccg cgtgtcttat gctacggagc ctgggctatc catgccgctt cgtcgagggc
     1921 ctctacgcac atccgcggcg gttcgacccc aagacgc
//
LOCUS       DATAQL010000263         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1247807, whole genome shotgun sequence.
ACCESSION   DATAQL010000263 DATAQL010000000
VERSION     DATAQL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247807"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..2392
                     /locus_tag="VHS97_04470"
     CDS             260..2392
                     /locus_tag="VHS97_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEX3447480.1"
                     /translation="MINPLIIPQTTQAQAICPYCGVGCRLWMEAAHGELIRVRGVADA
                     PANLGGICAKGATLPEVIHTPDRLMQPHVRMARDKELRPMSWPDALGWTAARFRAIID
                     RHGPDAVAFYGSGQLESEAGYLAVKLFKGSIGTNNTDSNSRLCMASAVAGYRTSLGAD
                     GPPCCYADIDLSDCLVVWGSNMAEAFPVTFDRVKAHMKANPGVELIVVDPRRTNTANH
                     ATLHVPVAPSGDIPLMNAVGRLLLELGAVEHDFVAAHTEGFGAYRDFLLHADWEGLVS
                     AAGVPESLIRALADRVARSRSWLTFYCQGLNQSTVGMWKNNSIINLHLLTGQIGKPGA
                     GPFSLTGQPNAMGGREVGLLAQSLPGYRFTDDPEHRAEVEAYWGRPAATVSTRPGLTA
                     VEMSRALEAGRLKAIWIAATNPAVSLPDLHQVRRALSRAELIVVQDPYHPTETTRFAD
                     ILLPTSQWAEKTFTSTTSDRLVSFSEQVVNPPGTALPDWQILARFGQIMGYSGFQFEH
                     SGAVWDEFIGLTAGRPCDMTGMKSERLQRERHLRWPCPSADHPGTERLYLDRKFPTES
                     GRARFLPRPHQPPRETTDHEFPMILTTGRLYAHWHTLTRTGKSRKLVQREPSPFVEVH
                     PDDAAKIGLVAGQLAELSTRRGLVQLPVRINPGLSRGVVFVPIHWGDQHRENVAANYL
                     TIPAIGRIAKQPEFKYCAVRLAPAFEVP"
     gene            2453..>2719
                     /locus_tag="VHS97_04475"
     CDS             2453..>2719
                     /locus_tag="VHS97_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447481.1"
                     /translation="MDLQSFRRAGHMPTLMCALLYFTVSCMAWMMVGALANSIIADLG
                     VVSDSHKGLLVAVPVLGGAILRLVLGPMADYMGAKKTALLGLVLT"
BASE COUNT          474 a          910 c          873 g          462 t
ORIGIN      
        1 tcccccggct ggcgaaagcc ggggaagaga cgcgccactg gggtaccgac gacgtgcgcg
       61 ccaagggtgg tgattatttt caggccaagt cttcgcccag aagccctgcg tcggctccgt
      121 gcggagcgcc tccagcctct ctgggagatc aaccgcccga ttcgggccgg tagacgtgta
      181 cacatttcga tgcgggcccc gttcaatctt gagcgattaa cgtctgggat ccgttaacga
      241 tgatgcccag gagaaagtga tgattaaccc gctcatcatc ccacagacca ctcaggccca
      301 ggcgatctgc ccttattgcg gggtgggctg ccggctctgg atggaggcgg cccacggcga
      361 gttaataagg gtcagagggg tggctgacgc gccggccaac ctggggggca tctgcgccaa
      421 aggggcaacc ctgcccgagg tgattcacac acccgaccgc ctgatgcaac cccacgtgcg
      481 gatggcgcgc gacaaggaac tccggccgat gagctggccc gacgcgctcg gctggactgc
      541 agcgcggttt cgagcgatca tcgaccgcca cggcccggac gcggtggcct tttacggcag
      601 cggccaactc gagagcgagg cgggctatct ggccgtcaag ctgttcaagg ggtcgatcgg
      661 caccaataac accgactcca acagcaggct ctgcatggcg tcggccgtgg ccggctatcg
      721 cacgagcttg ggcgccgacg gcccgccctg ttgctacgcc gacatcgacc tgagcgattg
      781 cctggtcgtc tggggcagca acatggcgga agccttcccg gtgaccttcg acagggtcaa
      841 ggcccacatg aaggcgaatc ccggcgtcga actgattgtg gtcgatcccc ggcggacgaa
      901 caccgccaat cacgctacgc tgcacgtccc agtcgcccct agcggcgata tcccgctcat
      961 gaacgccgtc ggccggctct tgctcgaact tggcgccgtc gaacacgact tcgtggcggc
     1021 gcacaccgag ggcttcgggg cctaccgcga tttcctcctg cacgcggact gggaagggct
     1081 ggtctcggcc gccggcgtcc cggagtcgct catccgggcc ctggccgacc gggtcgcgcg
     1141 ctcgcgctcg tggctgacat tctactgcca ggggctgaat cagagcaccg tcggcatgtg
     1201 gaagaacaac agcatcatca acctccatct cctgaccggt cagatcggta agccgggcgc
     1261 ggggccgttc agcctgaccg gccagcccaa tgcaatgggg ggccgcgagg tcgggctctt
     1321 ggcccagtcg ctgccgggct accgatttac cgatgacccc gagcaccgcg ccgaagtcga
     1381 ggcgtactgg ggccgcccag cggcgacggt ctcgacgaga ccaggcttga cggccgtcga
     1441 gatgtcccgg gcgttggagg cggggcggtt gaaggcgatc tggatcgcag cgaccaatcc
     1501 cgcggtcagc ctccccgacc tgcatcaagt acggcgcgcg ctgtccaggg ccgaactgat
     1561 cgtcgtccag gacccctacc acccgaccga gaccacccgc ttcgccgaca tcctgctccc
     1621 aacgtcgcag tgggccgaga agacgtttac ctcgaccacc agcgatcggc ttgtctcctt
     1681 cagcgagcaa gtggtcaacc cgcccggcac ggccctgccc gactggcaga tcctggcgcg
     1741 gtttggtcag atcatgggtt attcgggatt tcagttcgag cactcaggcg cggtctggga
     1801 cgagttcatc gggctgaccg ccggccggcc gtgcgacatg acggggatga agtccgagcg
     1861 gctccagcgc gaacgacacc tgcgctggcc ctgcccgagc gctgaccatc cggggaccga
     1921 gcggctctac ctcgaccgca aattcccgac agaaagcggc cgcgcacgct tccttccgcg
     1981 gccgcatcag ccgccgcgcg agaccaccga ccacgagttt ccaatgatcc tgacgaccgg
     2041 acggctgtat gcccactggc acaccctgac tcgcacgggc aagtcgcgca agctggtcca
     2101 gcgcgagccg tctccgttcg tcgaggttca ccccgacgac gcggccaaga tcggcttggt
     2161 cgcggggcag ttggccgaac tgagtacccg tcgcggcctg gtccagctcc cggtcaggat
     2221 caacccggga ttgtcgcgcg gcgtcgtctt cgtcccgatt cattggggcg atcaacaccg
     2281 agagaacgtg gcagcgaatt acttgacgat cccggcaatc ggccggatcg ccaagcagcc
     2341 ggaattcaag tattgcgcgg tccggctggc accagcgttc gaggtcccgt aggccggcgt
     2401 ggcctccatt ccaccgcgat cgcgtactag aacccgaacc gggagtcagc cgatggatct
     2461 ccaatcgttc cgacgcgccg gccacatgcc aacgctcatg tgtgcattat tgtatttcac
     2521 ggtcagttgc atggcctgga tgatggtcgg cgctctggct aactcgatca tcgccgacct
     2581 cggagtggtt tccgactcgc ataagggtct cttggtcgcg gtcccggtgc tcggcggggc
     2641 gatcttgcgc ctggtcttgg gccccatggc cgactacatg ggcgccaaga agacggcgct
     2701 cttggggcta gtgctgacg
//
LOCUS       DATAQL010000264         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5369904, whole genome shotgun sequence.
ACCESSION   DATAQL010000264 DATAQL010000000
VERSION     DATAQL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5369904"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1589
                     /locus_tag="VHS97_04480"
     CDS             285..1589
                     /locus_tag="VHS97_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447482.1"
                     /translation="MQPEWTGRKADAGDKSEATAPVRKWFTAELWTWLLVGVGVLIRL
                     VEYARNRPVYVDERLLLKNLTQLSVFDFHTILTEDQLAPPAFLVVERLLVRLPFPDVQ
                     TARLVPLLCGIASMFLMRSVARRFVLPHGVPIAVGLFALNDWLIYYASELKQYSSDAA
                     LTLVAFLLAAGRTEETGLESPRRLLALAGFGIVGVWFSHPLALVLGGVGSYLVVRAAL
                     RGEWRVVARLSGVILLWVCSFGLCFLVSRRILSKGDFLWNWWDFAFLPIPPHSIADLQ
                     RDFWQVANILNNPSWVVTPLGVLTSVFVALGLFLLGGLSMGLRWRGGVYVLVAPILFT
                     LIASALRQYPFHGRLLLFLIPSVHLLVGEGAAALSRRGGAIATFALGAFLLAQPAGHV
                     LWTQLVATRFHGGFDSHGDLLPDVLDYLEQLHREFPRRRSSP"
     gene            1586..3073
                     /locus_tag="VHS97_04485"
     CDS             1586..3073
                     /locus_tag="VHS97_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3447483.1"
                     /translation="MSVIDHQQADAQRLAPRGTSGRVRFSRDGLACGFIVVVAALLRF
                     FELGRLSFWYDEVVTMRLARTASPAALIEQLLQIDATRAPLHPLFLQAWIRIFGSSEA
                     AARSLSVVCGLATIVLIYEIGRVAFDTMTGLWASWLAAFSPLLVVYAREARMYGWLVF
                     VTCLCWRLLLGLRDNNSTARGLAYLVSLAALVYSHPLGLFMLGALALAGLIDLKACFG
                     GLKRWLVIHCGAMLLCLPWFRYYLDHAPEFLTGTLSPRFLLGTPIGFIGGNSLVMVGL
                     VLLIALGIARRGLYRDQEGCWHVVPKSMTAYGMLLVWLIVPPFALYVYSWVSYPVFGP
                     ARYTVFVAPAYLILVASGLSRIHAWARYPLALAVTAISAMALLPLVYAPDLKADWRSF
                     SAEIFRSLVERPRDSVLVIVASTDPDRNVEVETARYYLPEHCNVLGGEEATAERLESI
                     DPVDVYFTVGSRQGRPVSSVPARLGPYRFQDERRFAGLIVYRGTR"
     gene            complement(3055..>3388)
                     /locus_tag="VHS97_04490"
     CDS             complement(3055..>3388)
                     /locus_tag="VHS97_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447484.1"
                     /translation="VREGQRWSVFGSRNAVVRPDELGREAGKNLAEDPQVKGAFQIVE
                     MLGLGAVPAELKERSLRIGAATAKALGMARSAFDQDLDQLALPVLEPDRDARPNTDDA
                     NPAQRVPR"
BASE COUNT          495 a          995 c         1050 g          848 t
ORIGIN      
        1 tccatcttgt cgccgggcag gtgggtgaaa ccgagctctg ctccttccac gaggagtttc
       61 tgcgcgatcg cccaggcgat gctgaagtca ttcgccactc ccaagatcat tccttttttg
      121 cccgaaaaca gtcccatccc cggccccttt cgcctttacg gcgccaaaat gtcttctggc
      181 tcatccgcgc ttggtcgtca gcgttgtaaa ataccgggac tcaccggccc gctcaacgtc
      241 gactttgcac cggggctgga cgaggacggc gaaggggtat tttcatgcag ccagaatgga
      301 ccggacggaa ggcggacgcc ggcgacaagt cagaagcgac ggcgcccgtg cgaaaatggt
      361 ttacggccga actctggacc tggctgcttg tcggggtcgg cgtgctcatc cgcttggtgg
      421 agtatgcccg caatcgcccc gtgtatgtgg atgaaagact cctgctgaaa aacttgacac
      481 agctttcggt cttcgacttt cacacgattt tgaccgagga tcagcttgct ccacccgcct
      541 tcctcgtggt cgagcggctg ttggtacggc tgccgtttcc tgacgtgcag accgcccgac
      601 tggtcccgct cttgtgcggc atcgcgtcga tgttcttgat gcgttcggtc gcccgccggt
      661 ttgttttacc ccatggcgtc ccgatcgccg tggggctgtt cgccctcaac gattggctga
      721 tctattacgc gtccgagctc aaacaatata gtagcgacgc tgccttgacg ctcgtcgcat
      781 tcttgctggc ggccggacgc acggaggaga ctgggctaga gagcccgcga cgactgttgg
      841 cgcttgccgg attcggcatt gtcggcgtct ggttctcgca tccgctggcg ctcgtcctcg
      901 ggggcgtggg aagctacctc gtggtcaggg ctgcgcttcg cggtgaatgg agagttgtag
      961 cgcggctctc gggagtgatc ctgctgtggg tatgcagctt cgggctctgt ttcttggtct
     1021 cgcgtcggat tttgagcaag ggagattttc tctggaattg gtgggacttt gcgttcttgc
     1081 ctattccgcc acactcaatc gctgacctcc aacgagactt ttggcaggtg gccaatatcc
     1141 tcaacaatcc ttcatgggtg gtcacgccgc tcggtgtact cacttcggtg tttgtggcgc
     1201 tgggtctatt cttgttgggc gggctgtcga tgggattgag gtggcgtggt ggtgtttacg
     1261 tgctggttgc cccgatattg ttcacgttga tcgcttcggc actgcgtcag tatccatttc
     1321 atggacggct acttttgttt ctgattccgt ccgtgcacct gttagtcggc gaaggggcag
     1381 cggccttgtc gcgtcgtggt ggggcgattg caacgtttgc acttggtgca ttcttgctcg
     1441 cacagcccgc cggccacgtc ctgtggactc agttggtcgc aactcgtttt cacggcggat
     1501 tcgactcaca cggcgacctg ctgcccgatg tgcttgacta ccttgaacaa ctccatcgag
     1561 agtttccgcg tcgtcgatcg tcaccatgag cgtaatcgac catcagcagg cagacgccca
     1621 gcgattagct ccgcgcggca cttcagggcg cgtcaggttt tcgcgcgacg gtctggcgtg
     1681 cgggttcatc gtggtcgtgg cggctttgct gcggtttttt gagctcggcc ggcttagctt
     1741 ctggtacgac gaagtcgtga cgatgcgcct ggccagaacg gcaagcccgg ctgctttgat
     1801 cgagcaactt cttcagatcg atgcgacccg cgcgcccttg catccgctgt ttttgcaagc
     1861 ctggatccgt attttcggct cgtcggaagc agcagctcgc tcgttgagcg ttgtctgcgg
     1921 actcgccacg atcgtcttga tctacgagat cgggcgcgtc gccttcgata ccatgaccgg
     1981 tctctgggca tcatggcttg ccgcctttag cccgctactc gtcgtctacg cccgcgaggc
     2041 acgaatgtac ggatggctcg tcttcgtgac ctgtctctgt tggcggttgc tgctcggtct
     2101 gcgcgacaac aactcgaccg cccgtgggtt ggcttatctg gttagcctgg ccgcgctcgt
     2161 ttactcgcat ccgttgggtc tcttcatgct gggcgcactg gcgctggccg gattgatcga
     2221 ccttaaggca tgctttggcg gcctgaagcg ctggctggtg atccattgcg gtgcgatgct
     2281 gctatgtctg ccgtggttcc gctattacct cgatcatgcg cccgagttct tgaccggcac
     2341 cctatctccc cgtttcttgc tgggcacgcc gatcggcttt atcggtggga attctctggt
     2401 catggttggc ctggtgttgt tgatcgctct gggaattgcc cgtcgagggc tgtatcgcga
     2461 tcaggagggg tgctggcacg tcgttccgaa atcgatgact gcgtacggca tgttgctcgt
     2521 gtggctgatc gtgccgccgt ttgccctcta cgtttactcc tgggtgtcgt acccggtctt
     2581 cggtccagca cgctacacgg tgttcgtcgc tcccgcgtac ctgatcctgg ttgcctcggg
     2641 cttgagcagg atacacgcat gggctcgcta tccgctggcc ctggctgtga cggccatctc
     2701 tgccatggcg ctcttgccac tggtttacgc cccggatctc aaggcggatt ggcgcagttt
     2761 ctctgccgag atcttcagga gcctggttga gcgtccgcgc gattccgtat tggtcatcgt
     2821 cgcgtcgacg gatccggatc gaaacgtgga agtcgagacg gcccgctatt acttgccgga
     2881 gcattgcaat gtacttggcg gcgaggaagc aaccgctgag cggctcgaga gcatcgatcc
     2941 agttgacgtg tatttcacgg ttggatcgcg gcaggggcgg cccgtcagct cggtgccggc
     3001 tcgacttggt ccttaccgat ttcaggacga acggcgattc gcgggcttga tcgtttaccg
     3061 tggaacgcgt tgagcgggat tggcatcgtc ggtgttgggg cgagcgtcgc ggtcgggttc
     3121 gagaaccggc agcgccagtt gatcaaggtc ttgatcgaac gccgatcgcg ccattccgag
     3181 tgcctttgcg gtggcggctc cgatgcgcag gctgcgctcc ttgagctcgg ccgggacggc
     3241 tccgaggccc agcatctcca cgatctggaa agctcccttg acctgcgggt cttcggccag
     3301 gtttttaccg gcctcacgcc ccaattcgtc gggccgaacc acggcgtttc gcgatccgaa
     3361 gactgaccat cgctggccct cgcggacc
//
LOCUS       DATAQL010000265         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2622216, whole genome shotgun sequence.
ACCESSION   DATAQL010000265 DATAQL010000000
VERSION     DATAQL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622216"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VHS97_04495"
     CDS             <1..721
                     /locus_tag="VHS97_04495"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX3447485.1"
                     /translation="MLATGTDAVAGRQDVVPNIRYADDAINMPVQAATHWDSLGHIFY
                     QEAMYNGHDAREVDCNGLGKLGIEHAKSKMVGRGVLLDVARFRGMEFLPDGYGISNAE
                     LDDCAQAEGVTIGRGDFVIIRTGQLEKCLKTGWGTYAGGDAPGVKFENCYWCQDKEIA
                     AICSDTWGVEVRPNETKEANQPWHWVVIPAMGLTMGEMFYLKELGEDCAADRVYEFFF
                     CGPPLVITGGTGSPINPQAIK"
     gene            758..1222
                     /locus_tag="VHS97_04500"
     CDS             758..1222
                     /locus_tag="VHS97_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3447486.1"
                     /translation="MAQSAKPRAVGLNHVALEVGDIEEALAFYGRLFAFELRGKSDTM
                     AFIDLGDQFIALQKGRKQPADDGRHFGLVVDDKEAARVALQAAVVTPLEGPFLDFRDP
                     WGNRIEIVGYDNIQFTKAPNVLRGMGLTHLTKNENAQRELAEKGMALSQGDS"
     gene            1255..1608
                     /locus_tag="VHS97_04505"
     CDS             1255..1608
                     /locus_tag="VHS97_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3447487.1"
                     /translation="MIESVSAVTLATHDMARAVAFYRTLGFEIIHGGDDAAFTSFRAG
                     TGFLNLIAQPAERKWSWWGRVIFYHSDVDSLYASVIAAGYRPDTAPRDAEWGERFFHL
                     TDPDGHELSFAWPLR"
BASE COUNT          318 a          530 c          545 g          305 t
ORIGIN      
        1 aatgctcgcg accggcaccg acgcggtcgc gggccgccag gacgtggtcc ccaacatccg
       61 ctacgccgac gacgccatca acatgccggt gcaggccgcg acccactggg actcgctcgg
      121 ccacatcttc taccaggagg ccatgtacaa cggccacgat gcgcgtgagg tcgactgcaa
      181 cggcctcggc aagctcggta tcgagcatgc gaagagcaag atggtcggcc gtggcgtgct
      241 gctcgatgtc gcccgcttcc gcggcatgga gttcctgccg gacggctacg ggatttccaa
      301 tgccgagctc gatgactgcg cccaggcgga gggtgtcacc atcggccgcg gggacttcgt
      361 catcatccgc accgggcagc tggagaaatg cctcaagacc ggctggggta catacgccgg
      421 cggcgacgcg ccgggcgtca agttcgagaa ctgctattgg tgccaggaca aggagatcgc
      481 tgccatctgc tccgacacct ggggcgtaga ggtgcgtccc aacgagacca aggaggcgaa
      541 ccagccgtgg cactgggtgg tcatcccggc catgggtctc accatgggcg agatgttcta
      601 cctgaaggaa ctcggcgagg actgcgccgc cgatcgcgtc tacgagttct tcttctgcgg
      661 ccctcccctg gtcattactg gcggcaccgg ctcgccgatc aacccgcagg cgatcaagtg
      721 atcgccaagg ccgcgtgcgg tgcacggagg actccggatg gctcaatctg caaagccgcg
      781 cgccgtgggc cttaaccacg tcgcgctgga ggtgggagac atcgaggagg cactggcgtt
      841 ctatggccgc ctcttcgcgt tcgagttgcg cggcaaaagt gacacgatgg cctttatcga
      901 tctaggcgac cagttcatcg cccttcagaa aggccgcaaa cagcctgcag acgatggtcg
      961 ccatttcggc ctggtggtcg acgacaagga ggcggcgcgc gtcgccctcc aggctgccgt
     1021 cgttacgccg ctcgagggcc catttcttga cttccgcgac ccgtggggca accggattga
     1081 gatcgttggc tatgacaata tccaattcac caaagccccc aacgtcctcc gcggcatggg
     1141 tttgacgcac ttgaccaaga acgagaacgc gcaaagggag ctggccgaaa agggcatggc
     1201 gctcagtcag ggcgactcct aacgtgcctc ggactcgacc gacttagtgc cgcaatgatc
     1261 gagagtgtca gtgccgtcac gctggccacg cacgatatgg cgcgcgccgt tgccttttac
     1321 cgcacgctgg gatttgagat cattcatggt ggcgatgacg cggcgtttac aagctttcgg
     1381 gctggaaccg gctttctcaa tctcatcgca cagcctgcgg agcgaaagtg gtcttggtgg
     1441 ggacgtgtca tcttctacca ttccgacgtc gacagcctct atgcgagcgt gatcgcggcc
     1501 ggttatcgac cggacacggc accgcgcgat gccgaatggg gcgagcggtt cttccacctg
     1561 accgatcctg atggccacga actgagcttc gcttggccat tgcgatagtg agtgaatccg
     1621 gcaaggctgg ttgcctagcg ccccgcaccg tgacaggtgg ggcgctagcg cacgatgggg
     1681 ccgatccagg caggctcg
//
LOCUS       DATAQL010000266         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2622420, whole genome shotgun sequence.
ACCESSION   DATAQL010000266 DATAQL010000000
VERSION     DATAQL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622420"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VHS97_04510"
     CDS             <1..1315
                     /locus_tag="VHS97_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HEX3447488.1"
                     /translation="KTVLTELKQRGCFCVVGGPWVTVQEDYFGDLADVIFVGEAEDTW
                     PRFLEEWRQGLHQLRYEQIEKTDMTKVPTPRFDLLKMKHYAFGSLQFSRGCPFQCEFC
                     DIIVTFGRRPRIKTSAQVIAELEAIWKAGMDIVFVVDDNLIGNKKAIKSVLRDVVTWQ
                     EQRNYPLSLFTEASIDLADDPELMDLMVQANFIATFVGIESPDEESLREAKKFQNVRT
                     GGTLLEKVHRIQEAGMEVWCGMIMGFDNDDIGIFDRQIQFVQEARISFSMSGMLSAIP
                     KTPLHARLANEGRLDTADTNEYGTNVVPILISRGDLLQGYIRVLNELYDTEAYFDRTD
                     SLWLKPSFDIGANKKRSWLSTPRSYRQEAYFLAKSVGLFIRLMTRVRDPELRREYRQR
                     LWRFLKVHRRPGLALNYVFHMTMHYHAQYLAKRMASKELQLVNSF"
     gene            1358..2182
                     /locus_tag="VHS97_04515"
     CDS             1358..2182
                     /locus_tag="VHS97_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447489.1"
                     /translation="MSVFGESANGFPKLPILSPAEIPPPGQRGETARSQFEKYGGLFY
                     LGIAGLAVLAVLVGWFAFRLWNLRDVWTEVYVLHDPAHSELDRVRAAFRLAQDPRVND
                     AQRMEMSLKRDVPDLARYLLAESVSTDAMAHDPRSFALTVARSADWPDWLRLLLTRQL
                     AYGSARGYAIPREALTELASHSDPMIGLWADCGLTLLPQSDPKAAGAIEQAEVVPGPT
                     AELAALLRAATAAPPPVRETRLNDASLWLRHHHPQSAKIWQGWKIADGQIDRDGAN"
     gene            complement(2329..>3241)
                     /locus_tag="VHS97_04520"
     CDS             complement(2329..>3241)
                     /locus_tag="VHS97_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX3447490.1"
                     /translation="NRVQALFPVGATPLLNPVGTAPGIWMQINRAAIACLPGVPHEMK
                     LMFRDQVTPRLRANGLVNLVTVHRKINLFGKGESEIEAQAMDLTTRGRVPEVGITAHD
                     STISFRITATATSEDEARLLIEPTAELIYERFGSLVVGEGSDDVPEAVAAQLARANAT
                     LATAESCTGGVVAQMITAIPGVSRSYLGGVVSYANQAKIELLGVPTALIEAHGAVSAE
                     VASAMAEGVRTRLGADLGLATTGVAGPSGGTLEKPVGLVYLGLATSKGTQTRRLDIGS
                     DQPRDIIQRRSSKAALNWVRLTVLENR"
BASE COUNT          618 a          990 c          978 g          655 t
ORIGIN      
        1 gaagacagtc cttaccgagc taaagcagcg cggttgtttt tgcgtggtcg gtggtccgtg
       61 ggtcacggtc caggaagact attttggcga tttggccgac gtcatattcg tgggcgaggc
      121 tgaggataca tggccgcggt ttcttgagga atggcgtcag ggtcttcatc agctccggta
      181 cgagcaaatc gaaaagaccg acatgaccaa ggtcccgacg ccgcgcttcg acttgctcaa
      241 aatgaagcat tatgcgtttg gcagcctgca gttttcacgc ggctgccctt ttcaatgcga
      301 gttttgtgac atcatcgtca ccttcggccg ccgcccgcgc atcaagacca gcgcgcaagt
      361 gatcgccgag ctcgaggcga tttggaaggc cggcatggat atcgtcttcg tcgtcgacga
      421 taacctgatc ggcaacaaga aagcgatcaa gtctgttctg cgcgacgtgg tcacctggca
      481 ggagcagaga aactatccac tgtcgctttt caccgaagcc tcgatcgacc tggcggacga
      541 ccccgagctg atggatctga tggtccaggc caacttcatc gcaacctttg tcggcatcga
      601 aagccccgat gaagaatcac tccgggaggc caagaagttt caaaacgtcc gcacgggcgg
      661 aacgctgctc gaaaaagtgc accgcatcca ggaagccggc atggaggtct ggtgcggtat
      721 gatcatgggc ttcgacaacg acgatatcgg gattttcgat cgccagatcc agtttgtcca
      781 agaggcacgc atctcgtttt cgatgagcgg catgctctcg gcaattccca agacaccgct
      841 ccacgcgcgc ctggcgaacg aggggcgcct cgacacggcg gataccaacg agtatggtac
      901 caacgtcgtc ccgatcttga tcagccgcgg cgatttgctt caaggttata tacgggtctt
      961 gaacgagctc tacgataccg aagcgtactt tgatcggacc gattccctct ggctgaagcc
     1021 atcgttcgac atcggggcga acaaaaagcg aagctggctg tccactccgc ggtcgtaccg
     1081 tcaagaggca tacttcctgg ccaagtccgt gggacttttc atccggctca tgacccgcgt
     1141 ccgcgaccct gaactccggc gcgagtatcg ccagcggctc tggcggttcc tgaaagtcca
     1201 ccgcaggccc ggcctcgcac ttaactacgt gtttcacatg accatgcatt atcacgccca
     1261 gtacctggcc aaacgcatgg cgtcgaaaga gctgcagctc gtcaattcgt tctgatctat
     1321 gacggcgatt ttccccattg acgctgagga tcgaccgttg agtgtcttcg gtgagagtgc
     1381 caacgggttt cccaagctac caatcttgag tcctgccgag atcccgccac ccgggcagcg
     1441 cggcgaaacc gcgcgcagtc agtttgaaaa gtacggtggg cttttctatc tcgggattgc
     1501 cggactcgct gtgctggccg ttctcgtcgg ctggttcgct tttcgactct ggaacctccg
     1561 ggatgtatgg accgaagtgt acgtgctcca cgacccggct cactccgagt tggatcgcgt
     1621 tcgcgccgcg tttcgtctcg cccaagatcc cagagtcaat gacgcgcagc ggatggagat
     1681 gtcgctgaag cgagacgtgc cggacctggc tcgctacctt cttgccgagt cggtttccac
     1741 cgacgcgatg gcgcatgatc cacgtagttt tgccttgacc gtggcccgca gcgccgattg
     1801 gcccgactgg ctgcggctat tgctcacacg gcaactggcg tacgggtccg cgcgaggata
     1861 cgccattccg cgcgaggcgc tcacagagct tgcgagccac tccgatccta tgatcggact
     1921 ctgggcggat tgtgggttga cgctgctgcc acagtcggac ccgaaggccg ccggggcaat
     1981 cgagcaagcc gaggtcgtgc cgggccctac ggctgagctt gccgcgctct tgcgagcagc
     2041 caccgccgct ccgcctccag tacgagagac acgcctgaac gatgccagtc tatggctgcg
     2101 ccatcaccac ccgcagtcgg ccaaaatctg gcaaggatgg aagatcgccg acggacagat
     2161 tgatcgcgac ggcgcgaact gatcgtaagg gatctggcat attgcgcgca taagtgagcg
     2221 tttttcgcga ttatgtaggt tgggcacggc ccactagccc ctgccggtgg gcggtgccca
     2281 ccctacggat tcggacgacc gccaggataa gttcttccac gcggttcctt accgattttc
     2341 caggacggtc agcctcaccc agttgagagc cgccttcgac gagcggcgct gaatgatgtc
     2401 gcgcggttga tccgagccga tgtcgagccg gcgcgtctgt gtgcctttcg atgtcgccag
     2461 gccgaggtag accagaccga ccggcttttc cagggtacct cccgaaggac cggcgactcc
     2521 cgtggtggcg agacccaggt cggcgcccag gcgcgttcgt accccctccg ccatggccga
     2581 ggcgacctcg gcgctgaccg cgccatgcgc ttcgattagc gcagtgggta cgcccaggag
     2641 ctcgatcttt gcttgattgg cgtaactgac gacgccacca agataggagc gactgactcc
     2701 gggtattgcc gtgatcatct gcgcaaccac gccaccggtg catgactcgg ctgtggcaag
     2761 cgtcgcgttg gctcgggcaa gctgggccgc cacggcctcg gggacgtcat cggatccttc
     2821 tccgaccacg aggcttccaa accgctcata aatcagctcg gcagtcggct cgatcaggag
     2881 acgcgcctca tcttcactgg ttgccgtggc agtgatccgg aagctgatcg tcgagtcgtg
     2941 ggcggtgatt ccgacctcag gaactcggcc gcgcgtcgtc aaatccatcg cctgggcttc
     3001 gatctccgac tcccccttcc cgaacaggtt gatcttgcgg tgcacggtga ccaggttgac
     3061 caagccgttc gcgcgcaacc tgggagtgac ctgatcgcga aacatcaatt tcatctcgtg
     3121 aggcacaccg ggaaggcagg cgatcgcggc gcggttgatc tgcatccaga tcccgggcgc
     3181 ggtcccgacc gggttgagca ggggcgtggc ccctacgggg aagagggcct ggacgcggtt
     3241 g
//
LOCUS       DATAQL010000267         6019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2011384, whole genome shotgun sequence.
ACCESSION   DATAQL010000267 DATAQL010000000
VERSION     DATAQL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011384"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VHS97_04525"
     CDS             <1..279
                     /locus_tag="VHS97_04525"
                     /inference="COORDINATES: protein motif:HMM:NF021567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HEX3447491.1"
                     /translation="VSAFASAIRAQYPKCPATEATQIAEHACRKYSGRVGRTAAAKEL
                     SPEAIRMAVIAHIRHAHTNYDELLARYADRDLARQRVRDKVSAILGDW"
     gene            807..1178
                     /locus_tag="VHS97_04530"
     CDS             807..1178
                     /locus_tag="VHS97_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3447492.1"
                     /translation="MLLSRSSPVTVNTPCATANVAVTAGKTRRSSMSGPVRSTRGTRR
                     DRRWRPEAESYFRRALDVARRQGAKSLELRAALSLARLLRERGRPVESRQLVAEPFGW
                     FTEGWDTPDLQDARTLLEDLA"
     gene            1692..3893
                     /locus_tag="VHS97_04535"
     CDS             1692..3893
                     /locus_tag="VHS97_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX3447493.1"
                     /translation="MLFRTRPKGPRPKFLLRIETLEGRIALSSLIAISDASIAEGDSG
                     AVMMDFAVSRSGDLTVPVTAGYTTADGTARAGTDYTAETGTVTIPAGATSATIAIPVL
                     GNLLLQPDRTFSVTLTGVAGGATSAGFGVQQPFSTGFGPFSVAVGDFNGDGKPDLVVA
                     NDNANTVSVLLNTTAPGATTPSFAPGQDFATDPDPVCVVVGDFNLDNRPDLAIVEQSS
                     GLVSIRLNTTAPGAATASFAPAQNFRTGLDPVSAAVGDFNGDGEPDLAVANQFSNSVS
                     LLLNTTAAGSNTPNFAAKQDFATGSIPNSVAIGDFNGDNRPDLAVANEGSNRVSVLLN
                     ATAPRSVTLSFSPRTDYGTDSAPISVAVGDLNGDGMPDLAVTNFFSNSVSVLLNTTTA
                     GANTPSFAAKQDFATGLFPDSVAVGDFNGDAVLDLAIANSNSNSVSVLQNTTGLGAVA
                     LSFAAKQDFATGTEPASIALADFSGDGKNDLAVANFSSNTVSVLLNTAGPTVAITRAT
                     AVGTILDDDSPASVAPAGGDNQSAILGATFAAPLAVKVYNANGHLVQGVSVNFTAPAG
                     GAYGTFPGGQDGVTVITDADGCATAPAFTSNATAGSYVVTAQAAGLTTIVEFHLSNVY
                     SIDTPGNANQAKHSGSTIPLKLEITDDAGNNLGTIDLLVQALFVVDQYGNRVALQSPG
                     NANPGNMFQYDPRTGIYQFNLKTTGYSSGLYRLYFEVGDDPTLYSLSFGVS"
     gene            complement(4616..5299)
                     /locus_tag="VHS97_04540"
     CDS             complement(4616..5299)
                     /locus_tag="VHS97_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX3447494.1"
                     /translation="MGNGPAVAIGAERFDWIRKHLYVPAVSDVLDSLGFRDRVMHQRL
                     RPLDPSHCVIVGRARTFRWMDTDYVSDTDPYGLEIAAMDSLGPGDVAVHSTDAAGTNA
                     PWGELMTTVAKRNGAVGCVCDSNIRDCRKIIAMEFPVFYTGIRPLDSLGRGRVMAYDV
                     PVRCGGVLVNPGELVFADFDGVVVLPREMEDEVVARAFEKVTKENNSRRDLLSGDSLR
                     TVFDRYGVL"
     gene            complement(5418..>6019)
                     /locus_tag="VHS97_04545"
     CDS             complement(5418..>6019)
                     /locus_tag="VHS97_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX3447495.1"
                     /translation="ALVAAAREALGANVALLVDPGWMVVRSARDAIDLCHALEPYDIF
                     WLEDFLHPECYGGYAQVKAAGVKMRIAAGEQEATAWGFRELIQRGGIDVAQPDLSRCG
                     GFTHARKIAWEAEYAGVEVCPHAWLTDLLTAASLHYNAVLPTALFLEYNVCDNPMLRE
                     IIRNPVRLDSQGFIPVPQGPGLGIDVNEAAVRRFRVNGK"
BASE COUNT         1125 a         1923 c         1853 g         1118 t
ORIGIN      
        1 gtctccgcgt ttgcgagcgc gatccgcgcg cagtatccca agtgtccggc aacagaagcg
       61 acgcaaatcg cggaacatgc ctgccgcaaa tatagcggtc gagtcggccg caccgccgcg
      121 gccaaagagc tcagcccgga ggcgatccgc atggccgtga ttgcccacat ccggcatgcc
      181 cataccaatt atgacgagtt gctggcgcgg tacgcagatc gcgacctagc acggcagaga
      241 gtccgggata aagtttcggc aatcctcggc gattggtaac ggcccgacac gtccgaaaat
      301 caggctcttc cgcagcaaag tgccggtccc tggaatcttc cacctcttgc cgtgtcttga
      361 ggacacggcc gctagccgcg caatctggct gccacacctt tgatgtacgg tgttttgcgg
      421 atttcgttaa tgcacccatg tcgttatctt catggaatcc gttcgtagcg ctctgtcttg
      481 gcctgctttt agcctgttgt cgggttgttg tcgattttcg cttgcgaggc ttaccgccag
      541 taaattgaga gcgtgtgtcg agccgacaag acggtgcgga ttgcggagcg ttcccgggga
      601 gggtggccga gaggacgatc gaccagtcgg gccgtgcgag gctatcggcg cagagcgccc
      661 cggacgactg atgatcgtcc ggggcgcgtc gcacccctca tgcgtgcggt cacagcctgg
      721 tattggcagc cgtggccgtc ccgccctgga ccggcacggc gccacggttg accacgagcg
      781 tcccggccgc gtcgcgggag accgcgatgc tgttgtccag gtcatcgccc gtgaccgtca
      841 acaccccctg tgcgacggcg aacgtggccg tgaccgccgg caagacgcgg cgatcgagca
      901 tgtcggggcc ggtgaggtcg accaggggaa ctcgtcgcga tcgtcgctgg cggcccgagg
      961 cggagtcgta cttccgccgg gccctcgatg tcgcccgccg gcagggtgcg aagtccctgg
     1021 agctgcgggc cgcgctgagt ttggcgcgcc tgctgcgaga acgtggccgg ccggtcgaaa
     1081 gccgacaatt ggtggcggag ccgttcggct ggttcacgga gggatgggac acgccggacc
     1141 tgcaagacgc ccgtacttta ctcgaagacc tcgcgtaagt ggtgccgcgc attcacggag
     1201 ccggcgcgta tgagcaggag gtccgccgga ccagcatcgc tatggggcgc gctgctgaca
     1261 ttgtgccata cgaggcacgg attattcttc tcaagaatgt caataggtaa atcctgctta
     1321 aaccgaggcg tgggataaac cgttcgtggt ccttgaccac ttgggaggac atcggtgacc
     1381 gtgcgaggcc gtttcgacac ggcgggagga gttcggtgca caatcgcggc gcctttgtcg
     1441 cctcaagcga tcagaccgtg gtaccccagc gacagtgccc aatgattcca gaattttctc
     1501 gattgggatc cgggcagtgt cctagggttg caataggcgt cgtgacaccg ccggtcaacc
     1561 caatcaagcg gtggtgctac tcgagattgg gtaagcagag gtcttcgcat ccttcgactc
     1621 gctgcgcgag cgtgatcgca agccggtttg tgctggggat ttcgagttgt ataaggcgga
     1681 ggatcgttcg catgttgttc cgcacgcgtc ccaaaggccc gcggcccaag tttctgttgc
     1741 ggatcgagac cctggaaggc cggatcgccc tgtcgagcct gatagcgatc tccgacgcca
     1801 gtatcgcgga gggggattcg ggggcggtga tgatggattt cgcagtttct cgctcgggcg
     1861 acttgactgt gccggtgacc gctggctaca ccacggccga cggcaccgcg cgggcgggga
     1921 ccgattacac agccgagact gggacggtga ccatccccgc gggcgcgacc agcgctacta
     1981 tcgccatccc ggtactcggc aacctgctct tgcagcctga ccggacgttc tcagttacgt
     2041 tgacgggcgt agctggcggc gcaacatcgg ccggcttcgg cgtccagcag ccgttttcca
     2101 ccggtttcgg ccccttttcc gtagcggtcg gagacttcaa cggcgacggc aagcccgacc
     2161 tcgttgtcgc caacgacaac gcgaatacgg tgtcggtcct gctgaacacg actgcccccg
     2221 gagcgacgac tcccagcttt gcccccgggc aggactttgc cacggatccg gaccccgtct
     2281 gcgtggtggt gggggacttc aaccttgaca acaggcccga cctggcgatc gtcgagcaga
     2341 gctcaggcct cgtttcgatc cgcttgaaca cgacggcacc gggggcggcc accgccagct
     2401 tcgcgcccgc acaaaacttc cgcaccgggc ttgaccctgt atccgccgcg gtaggcgact
     2461 tcaatggcga cggcgaaccc gacctcgccg tcgctaacca gttctcgaac agcgtatcgc
     2521 tactcttgaa caccacggca gccgggtcga acacacccaa ctttgccgcc aagcaggact
     2581 tcgccactgg gtcgattccc aattcggttg cgattgggga cttcaacggc gacaacaggc
     2641 ccgacctggc tgtggcgaac gagggctcga acagggtgtc ggttctcttg aatgcgaccg
     2701 cacccaggtc ggtcacgctg agcttttcac ccaggacgga ctacggcaca gactcggccc
     2761 caatctcggt ggcggtgggg gatctaaacg gcgacggcat gcccgacctc gctgtcacca
     2821 acttcttctc gaacagcgtg tcggttctct tgaacacaac gacggcgggg gcgaacacgc
     2881 ccagctttgc cgccaagcag gacttcgcga cggggttgtt ccccgactcg gtggccgtgg
     2941 gagatttcaa cggagacgcc gtcctcgacc tggccatcgc caacagcaac tcgaacagcg
     3001 tgtccgtgct ccagaacacg acggggcttg gggccgtggc cctgagcttc gccgccaagc
     3061 aggactttgc gaccggaacg gagcctgcct ccattgcgct agcggacttc agcggtgacg
     3121 gcaagaatga cctggccgtc gccaacttca gttcgaacac ggtctcggtt ttgttgaata
     3181 cggcaggacc tacggtcgcg atcacccggg ccacggccgt cggcaccatc ctcgatgacg
     3241 actccccggc ctcggtcgcc ccggccggtg gcgacaatca gtcggccatc ctcggcgcga
     3301 cattcgccgc gccgctagcg gtgaaggtct ataatgccaa cggccacctg gtccaggggg
     3361 tgagcgtcaa cttcactgcc cccgccggag gcgcctacgg cacgttcccc ggcggccagg
     3421 acggcgtgac ggtgatcacc gacgccgatg gctgcgccac ggccccagcc ttcacgtcga
     3481 acgccaccgc cggcagctac gtcgtcactg cccaggcggc cgggctgacg accatcgtcg
     3541 aattccacct gtccaatgtt tacagcatcg atacccccgg caacgccaac caggccaagc
     3601 acagcggcag cacgattccc ctcaagctcg agatcaccga cgacgccggc aataacctgg
     3661 gcacaatcga cttgctggtc caggccctgt ttgtcgtcga ccagtatggg aaccgggtcg
     3721 cactccagtc cccagggaac gcgaatcccg gcaacatgtt tcagtacgac ccccggacag
     3781 gcatctatca attcaatctc aagaccaccg gctattcctc aggcctgtac aggctctact
     3841 tcgaggtcgg cgatgacccc accttgtaca gcctgagctt cggggtatcc tgatcgagcc
     3901 ggcacattcc caaatggccc cgtactcggg cacgcagcgc gaaccgccct gtcccccgat
     3961 gcctttacgt tgatgctatc gcccgatggc gaccacgctt ctggacagaa ctgaagtcgg
     4021 ggagtgtcga tgcctaaaag gaggccgtag ccgtcaaccg cgcccggctg ccttggctcg
     4081 gagcgaggca gtcgggccct cgaccagact attttcgcaa caaattggca agcgcacgtc
     4141 ccgtgtcagc ccaacgtaac tttagtgtta ttgagtgcgg gatggatcga actgtccgtg
     4201 caattcaagg ttttccagcg tcaactcagt gtgtttgctg tgcgacgtga aatccaggaa
     4261 accaggttcg agtcctaccg ctgctcgtga gctgcttaat tcttgtgcca agcaatgccc
     4321 cgccgcaggg cgagagcagt tcgcgagtcg tcatgggatc ggcggcggcg gcgcacgcgt
     4381 tgcgacgacc gccgcgcgca cgtcctcgtc ggtaacttta ttgagcgcgt cgagccgctt
     4441 ggtcacgtcg ttaactccgt agcgcgtcga ttgtgtgtgc ccgtgttcaa gccgtcgatc
     4501 cgttgcaata gaaaacggcg ttccggctcg gtgcaggggt aggtaagcgc cacattataa
     4561 gagacggtgg tctcggcact gcggccgagg cggcggagag aatctgcgcc taatctcaga
     4621 gaacgccgta gcggtcaaag acggttcgta gagaatcgcc gctcagcaga tcgcgtcggg
     4681 agttgttttc tttcgtgact ttttcgaaag cgcgggcgac aacctcgtcc tccatctcgc
     4741 gaggcaagac gacgacgccg tcgaagtcgg cgaagacgag ttcgcccgga ttcaccagca
     4801 caccaccgca gcgcacaggc acatcgtagg ccatcactcg ccctcgcccg aggctgtcga
     4861 gtggacggat tccggtgtag aagaccggaa actccatcgc gatgatcttg cggcagtcgc
     4921 gaatgttgct gtcgcagacg cagccgacgg ccccattgcg cttggcgacc gtggtcatta
     4981 actctcccca gggggcgttc gtgcccgcgg catcagtgga atgcactgcc acgtcgccag
     5041 gacccagcga atccatcgcc gcgatttcga gtccatacgg gtcggtgtca cttacgtagt
     5101 ctgtgtccat ccagcgaaac gttcgcgcgc ggcccacgat cacgcaatga gacgggtcga
     5161 gcggccgcag ccgctggtgc ataactcggt cgcgaaagcc aagcgagtcg agtacgtcgg
     5221 acacggccgg cacgtaaagg tgcttgcgga tccagtcaaa tcgctcagcg ccaattgcaa
     5281 cagcaggtcc gttacccatg aacgatcacc tccacacaaa cggctacata tgtctcgcga
     5341 tcacgcgatg tttaccaaac ccgcatacac caaggagcga atccgggttt ttggagttca
     5401 gggtcatcag attgatgtca tttcccgttc actcgaaagc ggcgcaccgc ggcctcatta
     5461 acatcgatgc ccagacccgg cccttgcggc acggggatga agccctggga atccagccgc
     5521 acgggattgc ggatgatttc gcgaagcatg ggattgtcgc agacgttgta ttccaggaag
     5581 agtgcggtcg gcagtacggc gttatagtgc aagctcgcgg ccgtgagcaa gtctgtgagc
     5641 cacgcgtgcg ggcacacttc cactccggcg tactctgctt cccacgcgat cttgcgcgcg
     5701 tgcgtgaagc cgccgcagcg cgagaggtcg ggctgcgcga cgtcgatacc gccgcgctgg
     5761 atcagctcgc gaaatcccca agccgtcgcc tcttgctctc ctgcggcgat ccgcatcttc
     5821 acgcccgccg ccttcacctg ggcgtagccg ccatagcact ccgggtggag aaaatcctcg
     5881 agccagaaga tgtcgtaggg ttccagcgcg tggcacaggt caatcgcgtc tcgcgcgctg
     5941 cgtaccacca tccaaccggg gtccacgagc aatgccacgt tcgccccgag cgcctcacgc
     6001 gcggcggcca cgagcgcga
//
LOCUS       DATAQL010000268         4182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_27069, whole genome shotgun sequence.
ACCESSION   DATAQL010000268 DATAQL010000000
VERSION     DATAQL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27069"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..615
                     /locus_tag="VHS97_04550"
     CDS             331..615
                     /locus_tag="VHS97_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447496.1"
                     /translation="MNNVNLTPMEEFAHDARHWARQTIIFTRQYGVVGANTPTEEQGQ
                     LSNVSDGSVEIIDQLWAKAQTESTGLTQTLRKFVWLVLEWSSEHLPRTGA"
     gene            753..1070
                     /locus_tag="VHS97_04555"
     CDS             753..1070
                     /locus_tag="VHS97_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX3447497.1"
                     /translation="MKSVAIIGGGFCGTLTAVNLARLSTDSLRVNIVNKQNPLGRGVE
                     YGTPRPEHLLHVVARNMSAFPDLPFHFAEWLRNRADFADHDQRNEPDWVQETPENLVE
                     YYN"
     gene            1132..2334
                     /locus_tag="VHS97_04560"
     CDS             1132..2334
                     /locus_tag="VHS97_04560"
                     /inference="COORDINATES: protein motif:HMM:NF005750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEX3447498.1"
                     /translation="MAKILCVLYDDPVDGYPKSYARDDVPTLTRYPDGQLLPTPSKID
                     FKPGALLGCVSGELGLRKFLEGSGHTLVVTSDKDGPDSVFEKELPDAEVVISQPFWPA
                     YLTAARIAKAPKLKLAITAGIGSDHVDLQAAMERGLTVAEVTYSNSISVSEHVVMMIL
                     SLVRNYIPSHEWVNKKGWNIADCVERSYDLEGMTVGTVAAGRIGLAVLKRLKPFDVKL
                     HYTDRHRLPDEVERELELTYHPNVESMVRVCDVVTINCPLHPETEHMFNAALIGKMKR
                     GAYIVNTARGKICDRDAIVRALEEGQIAGYAGDVWFPQPPPQDHPWRTMPHHGMTPHT
                     SGTSLSAQARYAAGTREILECWFEKRPIRERYLIVDGGKLAGAGAHAYSAGNATGGSD
                     EAARFKKG"
     gene            2382..3824
                     /locus_tag="VHS97_04565"
     CDS             2382..3824
                     /locus_tag="VHS97_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX3447499.1"
                     /translation="MRSWFRSEDWFAVWLGFAVVLIALPTAVGVDLLGWVSSPKIWLN
                     PIDAVRPISSKYAFLPAFASLVFTYLLVLGLVAACAAARKIELRSFIPSFTAIFWVST
                     VSLLIGHYAYIAQTPDKRLATGITWSLGLTGEAGYLVALLAGLVVANLLPSVALWLKP
                     AARPEWFIKTAVVLLGAGLGVKAAGAAGLVTSIMFRGLAAIIEAYLIYWALVYLIART
                     FFRFSREWAAPLASGISICGVSAAIVTGAAIRARPVVPIMVSSLVVIFSVVELLVLPL
                     AARELLWSEPLVAAAWMGLAVKTDGAAVASGAITDAMIRSRALGAGVAYEAGWILTTT
                     TTVKVFIDVFIGIWALVLSLVWTYGIERKPGQTVPVREVLKRFPAFVLGYFALFLALL
                     GLTLARPATAPGLAAATAQTEPLRGLFFAMTFFTIGMASDLKRLWAEGIGRLALVYAV
                     SLFGFIIWIGLAISWLFFHGVRPPIIPGGP"
     gene            3826..4077
                     /locus_tag="VHS97_04570"
     CDS             3826..4077
                     /locus_tag="VHS97_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447500.1"
                     /translation="MNERSHEPDTHLQPSLAEELAAIPYEPLLPAEKWLISVSLILGV
                     ILLAVLSWASATFYPAKPAAPVAQSHAPGAHRPTTRTFL"
BASE COUNT          780 a         1244 c         1285 g          873 t
ORIGIN      
        1 cctctcaccc ttggggcacg agaacatcaa cttcttgggg agatattcat tcgcgctgtc
       61 ggaatccgtc gcccgcggcg agttgcgaca gttccaaaac tcggaggacg cagggagcaa
      121 ggtcgggccg aacgcggctt aacccgactt tctgttccga tgattgtcgg accccatcta
      181 ttggttcact aagtgattgc gcgacggagc tgggtcggcc acgagcgacc ggtcctctgg
      241 ctgggcgcca gggcggtcgg cttgccaaag aggtaccctg gtccgaacaa ccgtaagacc
      301 gccgttaatg atccggcaga cgtgaccatt atgaataacg taaacttgac gccaatggaa
      361 gagtttgcac acgatgctcg tcattgggcg cggcagacga ttattttcac gcggcagtac
      421 ggtgtcgttg gcgcgaatac cccaaccgaa gagcaaggcc aactgtcgaa tgtgagcgac
      481 ggatccgtcg aaatcatcga tcaattgtgg gccaaggccc agaccgaatc gactggcctt
      541 acgcaaactt tgcgcaagtt tgtctggctg gttctcgaat ggtcatcgga acatctgcct
      601 cggaccggag cgtgaccttg gttccgcgga gcacttgtgt tgtttgcgcg tcgtcgccgg
      661 tcaaccacgg atcgcgttca caagcggtgt ggtcaacgtt ctctagaaac cgagtacgaa
      721 cggcatgtgg acggcgtggg agctcatgag ccgtgaagtc agttgcaatc atcggcggcg
      781 gattctgtgg cactctcact gccgtcaatc tagcccgttt gagtaccgat tctctacgcg
      841 tcaacatcgt taacaaacag aatccgttgg gccgcggggt cgagtacggg acgccgcgcc
      901 cggaacacct cctccatgtg gtcgcccgca atatgtcggc gttcccggat ctgccgtttc
      961 atttcgccga atggctccgc aatcgggccg acttcgcgga ccacgaccaa cggaacgagc
     1021 cggactgggt gcaagagacg ccagagaatc tcgtagaata ctacaactga ttgcgagagc
     1081 ctgctcattg tcggattcga cgacgccgca actctttctc aggagaacgt aatggcaaag
     1141 atcctctgcg tcctgtacga cgatccggtt gatggttacc cgaaatcgta tgcgcgggat
     1201 gacgttccca cgctgacacg ctatccggac ggccaattgc tgccaacgcc gtcaaagatc
     1261 gacttcaaac cgggggcgtt gctgggctgc gtttccgggg aacttggact gcgcaagttc
     1321 ctggaaggca gcggacacac actcgtcgta acatcggaca aggatggccc cgactctgtg
     1381 ttcgagaagg agttgccgga cgcggaagtg gtgatctccc agcccttctg gccggcttat
     1441 ctcacggcgg cgcggatcgc caaagcgccc aagctcaagc tggcgatcac cgcgggaatc
     1501 gggtcggatc acgtcgatct gcaagccgcg atggagcgag gcctgacggt cgctgaagtc
     1561 acctacagca acagtatcag cgtctcagag cacgtcgtga tgatgatcct gtcgctggtg
     1621 cgcaactaca tccccagcca cgagtgggtg aacaagaagg gctggaacat tgccgactgc
     1681 gtcgagcggt cgtacgacct ggaagggatg accgtgggga cggtcgccgc cgggcgaatc
     1741 ggcctggcgg tgctgaagag gctgaagccg ttcgacgtca agctgcacta caccgatcgg
     1801 caccgccttc cggatgaagt cgagagagaa ctcgagctga cttaccatcc gaacgtcgag
     1861 tcgatggtgc gcgtctgcga tgtcgtcacg attaactgtc cgcttcatcc cgagacagag
     1921 cacatgttca acgcggcgct catcggcaag atgaagcgag gcgcatacat cgtcaatacc
     1981 gcgcggggga agatctgcga ccgcgacgcg attgtgcgcg ccctggaaga gggccagatt
     2041 gccggatatg cgggtgacgt ttggttcccg cagccgccac cgcaggacca cccttggcgg
     2101 acgatgcccc atcacggcat gacaccccac acatcgggta cgagtctgtc ggcgcaggca
     2161 cgttacgccg cggggacccg cgagatactg gaatgctggt tcgaaaaacg cccgattcga
     2221 gagcgatact tgatcgtcga tggtgggaag ctggccgggg ctggggcaca cgcctatagc
     2281 gccggcaatg caaccggcgg ctcggacgaa gcagcccgat tcaagaaggg atgacgtctc
     2341 aaagttggaa cggtttgctc taaattcggg gaagatgaac cttgcgatcc tggtttaggt
     2401 cggaagactg gtttgcggtg tggctcggtt tcgcagtcgt cttgattgcg ttaccgaccg
     2461 cggtgggcgt cgatctgctg ggctgggttt cttccccgaa aatctggctc aacccgatcg
     2521 acgccgttcg tcccatctca tccaaatacg cgttcctgcc tgcgtttgcc agcctcgttt
     2581 tcacgtatct gctcgtactc gggctcgtcg cggcttgcgc tgcggcccga aagattgagc
     2641 tacgcagctt catcccgagt ttcacggcga tcttctgggt cagcacggtg agcttgctga
     2701 tcggacatta cgcatatatc gcgcagacgc ccgataaacg gctagctact ggcatcacgt
     2761 ggtcactggg tctgactgga gaggcgggtt acctggtcgc gctcttggcc ggcctggtcg
     2821 tagcgaacct cttgccttcg gtcgccctat ggctgaagcc ggcggcacgg ccggagtggt
     2881 tcatcaaaac cgccgtcgtg cttctcggag cggggctcgg cgtcaaggct gccggtgcgg
     2941 cgggcctggt tacatcgatc atgttccgcg gtctcgcggc aatcatcgag gcgtatctga
     3001 tttactgggc gctggtctat ctgatcgcac ggacgttctt ccggttcagc cgggagtggg
     3061 cggcgccgct tgcgtcgggg atctcgatct gcggcgtctc ggccgcgatc gtcaccgggg
     3121 cggcgatccg tgcgcggccg gtcgtcccga tcatggtttc ctcgctggtg gtcattttct
     3181 cggtggtgga gctgctggtc cttccgctcg ccgcccgtga gctcctctgg agcgagccgc
     3241 tggtggcagc cgcatggatg ggccttgcgg tcaagaccga cggtgcggca gtggcgagcg
     3301 gggcgatcac cgacgccatg atccgctccc gcgccttggg agcgggggtc gcctacgagg
     3361 ctggctggat cctgacgaca accaccaccg tgaaggtgtt catcgacgta ttcatcggca
     3421 tctgggcgct ggttctgtcg ttggtctgga cgtacggaat tgaacgaaag cctggccaaa
     3481 ccgtcccggt gcgcgaggtg ctaaagcgat tccccgcgtt cgtcttggga tatttcgccc
     3541 tcttccttgc tctgctcggc ttgacgctgg cccggccggc aacggctccg gggctcgccg
     3601 cagccactgc ccagacagag cccttacgag gtttgttctt cgcgatgacg tttttcacga
     3661 tcgggatggc ttccgacctc aaacgtcttt gggccgaagg catcggccgg ctcgccctgg
     3721 tctacgccgt cagcctcttc ggctttatca tctggatcgg attggcgatc tcgtggcttt
     3781 tcttccatgg agttcggcca ccgatcattc cgggaggtcc ttgaattgaa cgagcgttcc
     3841 cacgagcctg acacgcatct gcaaccgtcg ctcgcggaag aactcgccgc gatcccgtac
     3901 gaaccgctac tgccggccga aaaatggcta atctcggtga gcctgatcct cggagtcatc
     3961 ctcctcgccg ttctttcatg ggccagcgcg acattttacc cggccaagcc tgctgcgcct
     4021 gtcgcgcagt cacatgcgcc gggtgcgcat cgaccgacga cccggacatt cctgtagcta
     4081 cgtcgcaatt gatctcctat cacgggtccg gccagacctc ctcctcgacc tggtatcaca
     4141 aggcagactg gctcgacttc aatacgattc agtccgggca ta
//
LOCUS       DATAQL010000269         5631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3539244, whole genome shotgun sequence.
ACCESSION   DATAQL010000269 DATAQL010000000
VERSION     DATAQL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3539244"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..2590
                     /locus_tag="VHS97_04575"
     CDS             125..2590
                     /locus_tag="VHS97_04575"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX3447501.1"
                     /translation="MAKTVNLLERYECGPFRFADDDYYDRHLVFDHVVALEKANTRQR
                     FEAMASAIRDLLTQRWLLTGQTHDRQNPKQVYYLSMEFLIGRTLANNIANLQVEEFVR
                     NDFASDAGHDWQALFEQEPDAGLGNGGLGRLAACFIDSLATLQIPAIGYGLRYEYGMF
                     RQEIDDGRQVEHPDNWLIHPDPWEVVRPSQTVDARLDCAFQMREGRLTVVPNVPTILR
                     GIPYDRPVVGYGGRTINALRLWGAAAHDDFNFLEFSRGDFFDAVHEKVAAEVVTRVLY
                     PDDSTPRGRNLRFVQEYFLVACSLADIVARFRRRGNDWKALPDKVAIQLNDTHPAMSV
                     AELMRILLDDAKLGWDEAWDITRRTLAYTNHTLLPEALETWPISLFETMVPRLLDIIY
                     EINRRFLDDVRARFPDDPGRLSRISLIAEGPEKRVRMANLAIVGTHSTNGVAEIHSRL
                     LRTTVVSDLATIFPERFNNKTNGVTPRRWLLLANPDLSRLISGAIGDGWVSDLSQLKK
                     IVPLADDASFRGGFRQAKRAAKTRFADWLKTTTGLAVDPDTIFDSQVKRIHEYKRQLL
                     NALHIIILYNRFRDNPKLDEPPRTFFFAGKAAPAYTLAKLIIKLINNVASVVNVDPVV
                     GDKLKVFFLPEYNVSLAQRLIPASDVSEQISTAGFEASGTGNMKFMMNGALTIGTRDG
                     AVIEMAAEAGEENFFLFGLSADQVAASQGWYNPRWHYEHEPETSRALDMIAANAFSPY
                     EPGIFGPICDTLLPRGDHYMHLADLASYVRAQEQVGSLYVKPDEWTRRAIKNVGHSGK
                     FSSDRTISQYASEIWNAKPCPVE"
     gene            2627..4267
                     /gene="pgm"
                     /locus_tag="VHS97_04580"
     CDS             2627..4267
                     /gene="pgm"
                     /locus_tag="VHS97_04580"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEX3447502.1"
                     /translation="MAIHPLAGQLAPANVLIDVPALEHAYYSNKPDPANPRQRVAFGT
                     SGHRGTPLDSTFTEAHILAITKAICDYRRASGIDGPLFLGKDTHAVSDAAQRTALEVL
                     AAEGMDVILERDDGVTPTPVISHAILVYNRGRTEHMGDGIVITPSHNPPADGGFKYNP
                     PNGGPADSEVTSWIQDRANLLLRAGNAEVKRISFPEALRAGTTHEVDFVKPYVDDLAS
                     VIDMDVLRTAGLKLGVDPLGGAAVHYWQPIADTYGLDITVTNPAVDPTFRFMTLDHDG
                     QIRMDCSSPYAMASLVKLKDGFDLAFGNDPDSDRHGIVTRSLGLLNPNHYLAVAIRYL
                     LTHRPDWSAHAMVGKTLVSSSLIDHVVKSLNRKLCEVPVGFKWFAPGLLDGSFCFGGE
                     ESAGASFLRRDGRVWTTDKDGPILDLLAAEILARTGKDPGEHYRELTEQFGIPYYTRI
                     DAPASPEQKAQLQKLDPSNVKASDLAGEPITAKLTRAPGNNAPIGGLKVVAQSGWFAA
                     RPSGTENIYKIYAESFRDQNHLDAIVAAAQQIVTDALT"
     gene            4339..>5631
                     /gene="pgi"
                     /locus_tag="VHS97_04585"
     CDS             4339..>5631
                     /gene="pgi"
                     /locus_tag="VHS97_04585"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX3447503.1"
                     /translation="MSTTALRDRPAWKALEAHYAKMRDVHLRRLFADDPGRGERLTAE
                     AAGIFLDYSKNRVTDETLGLLVKLAEESGLRARIDAMFRGDKINVTEDRAVLHVALRA
                     PKGASIVVDGQNVLPEVHAVLDRMAEFAGKIRSRAWKGYTGKPIKNIVNIGIGGSDLG
                     PVMAYEALKHYSDRNLTFRFVSNVDGTDFAEATRDLDPAETLFIISSKTFTTLETMTN
                     AQTARAWSLATLKSEQAVAKHFVAVSTNEQEVSKFGIDTANMFGFWDWVGGRYSMDSA
                     IGLSTMIAVGPDNFRAMLAGFHAMDEHFRTAPFERNLPVLLGLLTVWYTDFLGAETIA
                     VLPYDQYLKRFPAYLQQLTMESNGKSVSLDGSRVCYSTGPVYWGEPGTNGQHSFYQLI
                     HQGTRLIPCDLIGFAHTLNPLGDHHDLLMSNVFAQSEAL"
BASE COUNT         1119 a         1814 c         1600 g         1098 t
ORIGIN      
        1 ctcagtgtgc tcgggtacga gcattcccgg gatgaaccag tcatccgcct ctggaacgac
       61 gaccgccatg tgacgacctg aaccaagtgc aatgcagccc cgaccgattc cccggagcct
      121 cggcatggcc aagaccgtca accttctcga gcgctatgag tgcggcccgt tccgcttcgc
      181 tgatgacgac tactacgacc gtcacctggt cttcgaccat gtcgttgcgc tcgaaaaggc
      241 taacacgcgt cagcgcttcg aggcgatggc cagcgcgatc cgcgacctgc tcacgcagcg
      301 ctggcttttg acgggacaga cgcatgaccg ccagaatccc aagcaggtgt actacctatc
      361 gatggagttt ttgatcgggc gaacgcttgc caacaacatt gccaatctgc aagtcgagga
      421 gttcgtccgc aacgacttcg cttcggacgc cggccatgac tggcaggctc tcttcgagca
      481 agaaccagac gccgggctgg gcaatggcgg cttgggccgg ctggccgctt gttttatcga
      541 ttcgctggcg acgctccaga ttccggccat tggctacggc ttgcggtatg aatacggcat
      601 gttccgccag gagatcgacg acggccgcca agtcgaacat cccgataact ggttgatcca
      661 tcccgatccc tgggaggtcg ttcgacccag ccagaccgtc gatgctcgcc tcgactgcgc
      721 atttcagatg cgtgagggtc ggttgaccgt ggtacccaat gtgccgacga tccttcgcgg
      781 aattccctat gatcgcccgg tcgtcggtta cggcgggcgg acgatcaacg ccctgcggct
      841 ctggggcgct gccgcgcacg acgacttcaa tttcctggaa ttcagccgcg gcgacttctt
      901 cgacgccgtc catgagaagg tcgccgccga ggtagtgacc cgcgtcctat atccggacga
      961 ttccactccg cgaggacgca atctgcggtt cgtccaggag tattttctgg tcgcctgctc
     1021 gttggcagac atcgtggcgc ggttccgccg gcgcggcaat gactggaaag ccctccccga
     1081 caaggtcgcc atccagctca acgacacaca ccccgcgatg agtgttgccg agctcatgcg
     1141 catcttgctc gacgacgcca aattgggatg ggacgaagcg tgggatatca ccaggcgcac
     1201 cctggcctac accaaccaca cgctgttgcc cgaggccctc gaaacctggc cgatcagtct
     1261 cttcgagacg atggtgcctc gtctcctcga catcatttac gagatcaacc gccgcttcct
     1321 tgacgacgtt cgcgctcgtt tcccggacga tcccggccgt ctctctcgca tcagcttgat
     1381 cgctgaaggg ccggaaaagc gggttcgcat ggccaacctg gcgattgtcg gcacccacag
     1441 caccaacggc gtcgcggaaa tccattcacg gctgctgcgc accactgtcg tgtccgacct
     1501 cgcgacgatc ttcccggaac ggttcaacaa caagaccaac ggcgtgaccc cgcgccgctg
     1561 gctgcttttg gcaaaccccg atctatcgcg cctgatttcg ggcgcgatcg gcgacggctg
     1621 ggtcagcgat ctttcccagc tcaaaaagat cgtccccctg gccgacgacg cgtcgttccg
     1681 cggcggcttc cgccaggcca agcgggccgc caaaacgcgc tttgccgact ggctcaagac
     1741 gacgaccggc ctcgctgtcg atcctgacac catcttcgac agccaggtga aacggatcca
     1801 tgaatacaag cgtcaactac tcaatgcgct tcacatcatc attctttaca atcgcttccg
     1861 cgacaacccc aagcttgacg aaccgcctcg gacattcttt ttcgccggta aagccgcgcc
     1921 ggcctataca cttgctaaac tgatcatcaa actgatcaat aacgttgcct ccgtggtcaa
     1981 cgtcgatccc gtcgtgggcg acaaactcaa ggtcttcttc ttgccggaat acaatgtcag
     2041 cctggcgcaa cggttgattc cggcgagtga tgtctccgaa cagatctcca ccgccggctt
     2101 cgaagccagc ggcacgggca atatgaagtt catgatgaac ggggcgctca cgatcggcac
     2161 gcgcgacggc gctgtgatcg aaatggccgc ggaggccggc gaggaaaact tcttcctctt
     2221 tggtctgtcc gccgaccagg ttgccgccag ccagggatgg tataacccgc ggtggcatta
     2281 cgagcacgag ccagagacca gccgcgcgct cgacatgatt gcggccaacg ctttcagtcc
     2341 gtacgagccg ggcatcttcg ggccaatctg tgatacgctg ctgccgcgcg gcgatcatta
     2401 catgcatctg gccgacctcg cgtcttacgt ccgggctcag gaacaggtgg gttcgcttta
     2461 cgtaaagccc gatgagtgga cccggcgggc aatcaagaac gtgggtcatt ccggcaaatt
     2521 ttcgagtgac cgcacgatct cgcagtacgc cagcgagatc tggaatgcaa aaccgtgccc
     2581 agtggaatga gggcagtgcc gctgaaattg gcaagaggaa actctgatgg cgatccatcc
     2641 cttggccggt caactggctc ccgcgaacgt cctgatcgat gtccccgcgc tcgaacacgc
     2701 ttattactcg aataagcccg atccggccaa tccgcgccag cgcgtcgcct ttggcaccag
     2761 cgggcaccgg ggcacgccgc tcgacagcac atttaccgag gcgcacatcc tggcgatcac
     2821 caaggcgatc tgcgactacc gccgggctag cggaatcgat ggcccgcttt tcctcggcaa
     2881 ggacacgcat gccgtgtccg atgccgccca gcggacggcg ctcgaggtgt tggccgccga
     2941 gggcatggat gtcattctcg agcgcgacga tggcgtgacg ccgacgccgg ttatctctca
     3001 cgcgatcctc gtatacaacc ggggacgcac cgagcacatg ggtgacggca tcgtgatcac
     3061 gccttcacat aacccccccg ccgacggtgg cttcaagtac aacccgccca acggcgggcc
     3121 ggccgattcg gaggtgactt cttggatcca ggaccgcgcc aatctattgc tccgcgcggg
     3181 caatgccgag gtaaagcgaa tctcgtttcc agaagccctg cgagccggca cgacccatga
     3241 ggtcgatttt gtcaaaccgt atgtcgacga cttggcgagc gtgatcgaca tggacgtgct
     3301 gcgcaccgcc ggtctcaagc taggcgtcga cccgctcggg ggcgcggccg tgcattactg
     3361 gcagccgatt gccgacacgt atggcctcga tatcacggtc accaacccag ccgtcgaccc
     3421 gacgttccgg ttcatgacgc tcgatcacga tgggcagatt cgcatggact gttctagccc
     3481 ctatgccatg gcgagcctgg tcaagctcaa ggacgggttc gacctggcct tcggcaacga
     3541 tcccgactcc gaccggcacg ggatcgtcac ccggtcgctc ggtctcttga atcccaacca
     3601 ttacctggcc gtcgcgattc gctacctgct cacccaccgg cccgactggt ccgcccacgc
     3661 catggtcggc aaaacgctgg tcagcagtag cctgatcgat catgtcgtga aaagcttgaa
     3721 ccgaaagctc tgcgaggtgc cggtcggttt caagtggttt gcgcccgggc tgctcgatgg
     3781 ctcgttttgc ttcggcggcg aagagagtgc cggcgccagc tttttgcggc gtgacggcag
     3841 ggtatggacc accgacaagg atggaccgat actcgatttg ctggccgccg aaatcctcgc
     3901 acgtaccggc aaagaccccg gtgagcacta ccgtgagctg accgagcagt tcggcatccc
     3961 gtactatacg cggatcgacg caccggcctc gcccgagcaa aaagcacagc ttcagaaact
     4021 cgatccctcg aacgtcaagg catcagacct ggctggcgag ccgatcacag ccaagctgac
     4081 tcgcgccccg ggaaataacg cgcccatcgg cgggctcaag gtcgtcgccc agagcggttg
     4141 gtttgccgcg cggccatcgg gcaccgaaaa tatctacaag atctatgccg agagctttcg
     4201 cgaccagaac cacctggacg ccatcgtcgc cgcagcccag cagatcgtca ccgacgcgtt
     4261 gacatgatcg atcgagctca atcagaagtc gtgcgttttc cgttctgaaa tttcgatact
     4321 cgagtcggga atttcatcat gagcacaacc gcattgcgtg atcggcccgc gtggaaagcc
     4381 ctggaagcgc actatgccaa gatgcgcgat gtccatctcc gccgcctttt tgccgacgat
     4441 cccgggcgcg gggagcggct cacggcggag gccgcgggca tctttctcga ttactccaag
     4501 aaccgcgtga ccgacgaaac attgggtcta ctggtaaaac ttgccgagga atccggcttg
     4561 cgcgcccgca tcgacgccat gttccgtggc gacaagatca acgtcaccga agatcgtgct
     4621 gtgttgcacg tggccctgcg cgctcccaag ggcgcctcca tcgttgtcga tggtcagaac
     4681 gtgctacccg aggtccacgc cgtgctcgat cgcatggccg agtttgccgg caagattcgg
     4741 agccgggcct ggaaaggata cacgggcaag ccaattaaaa atatcgtcaa cattggcatc
     4801 ggcggctctg accttgggcc agtgatggcg tacgaggcac tcaagcatta cagcgaccgc
     4861 aacttgactt ttcggtttgt ctcgaatgtc gatggcaccg acttcgccga ggccacgcgc
     4921 gacctcgacc cggccgagac gcttttcatc atctcctcga agacgttcac gaccttggag
     4981 acaatgacca acgcccaaac cgcccgcgcg tggagcttgg ccactctcaa aagcgagcag
     5041 gcggtcgcaa aacatttcgt cgcggtttcg accaacgaac aagaagtcag taagttcggt
     5101 atcgacactg cgaacatgtt cgggttctgg gactgggtcg gcggccgtta ctcgatggat
     5161 tcggccatcg gcttatcgac tatgatcgcg gttggccccg acaacttccg cgcgatgctc
     5221 gccggcttcc acgcgatgga cgagcatttc cgaaccgctc cgttcgagcg caatcttccc
     5281 gtcctgctcg ggctcttgac cgtctggtac accgactttt tgggcgcgga aacgatcgcc
     5341 gttttgccgt acgaccaata tctcaagcgt ttcccagcat atttgcaaca gttgacgatg
     5401 gagagtaatg gcaagtcggt ctcgctggac ggcagccggg tctgctattc gaccggacct
     5461 gtttactggg gcgagccggg cactaatggc caacattcgt tttaccagct catccaccag
     5521 gggactcgcc tgatcccctg cgacttgatc ggattcgctc acacgcttaa ccccttgggc
     5581 gaccatcacg atctcttgat gtccaacgtg ttcgcccagt ccgaggccct c
//
LOCUS       DATAQL010000270         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3539259, whole genome shotgun sequence.
ACCESSION   DATAQL010000270 DATAQL010000000
VERSION     DATAQL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3539259"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VHS97_04590"
     CDS             <1..516
                     /locus_tag="VHS97_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEX3447504.1"
                     /translation="VNGKRFVDITASSGTGHLQKGHGVSFADWDSDGDLDLFVESGGA
                     VPGDKAYNVLFQNPGHGRHWLKVKLVGTKTNRSALGARIQVDVVHNDGTTQSIHRQVG
                     GGSSYGGNSLVESIGLADAQAVAALSVTWPVSRTRQFFRNIAADQTIEVIEGDDLPRF
                     LHTSPIVHRQP"
     gene            complement(546..890)
                     /locus_tag="VHS97_04595"
     CDS             complement(546..890)
                     /locus_tag="VHS97_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX3447505.1"
                     /translation="MPGEQQSTSTSVETEVTTDQRTRRLPPYNVVILNDEDHTFQYVI
                     ELLIKLFRHPLPKAEELTLRIHTTGRAIVYTTHKELAELKRDQVIAYGPDPRSKSSKG
                     PLRCYVEPAPTD"
     gene            1099..1437
                     /locus_tag="VHS97_04600"
     CDS             1099..1437
                     /locus_tag="VHS97_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447506.1"
                     /translation="MKGTSPGAALRDLQRAHAGLKKSRGILKLAKDDPGATAAAFAAG
                     WESLTQAHRIMAAISAEVVDDAVLTKQLALQRYATSLLVRLRRLKRSGSAGGVAADDD
                     DSIDDEDGED"
     gene            1444..2697
                     /locus_tag="VHS97_04605"
     CDS             1444..2697
                     /locus_tag="VHS97_04605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX3447507.1"
                     /translation="MARLKKLRSPVDAAPRMPDRSLTADEPLTTVAIRAAGTHQFVYR
                     KMVEGPVGPAAPNHGDLVRVVDRDGQRLGYALWNGRSQISLRFLSRQENPPGVEFWSR
                     RIDRAVALRTKVLNLDSQTSAYRVVHAEGDGLSGLVVDRFDDVLSVQCFSLGMYQRIG
                     PILALCAEKLGTAHFRVSVDERVAMQEDFSGRQLVSPQLPPRVTIIENGIRYRIHFEH
                     GHKTGFFCDQRDNRRDLARFSADKSVLDLCCYTGGFGLNALVRGNAREVTCVDLDEKA
                     VALARENGHANQVRLEVVHADAFGYARQMGLNGKTFGVVVLDPPKLIPDREEIAPGKR
                     KYFDLNVLAMKLVEPGGLLVTCSCSGLLDVQEFLALLGAAARNAERHVQLLSLVGAAA
                     DHPVAMSALEGSYLKVAWLRVDEQP"
     gene            2752..3192
                     /locus_tag="VHS97_04610"
     CDS             2752..3192
                     /locus_tag="VHS97_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX3447508.1"
                     /translation="MKFANVLLLLASAGVLVADEPKKDDAESIKGNWTAVSIKQGKQS
                     APDDFVKSFKFGLDGKNYTNTIGTQVMEEGGYTIDASKTPKTIDFDIKKGPGAGKKQL
                     GLYKIEGEKLTIVASLAGSAERPKSLDAGDADELAVVVLKRVKP"
     gene            3656..>4969
                     /locus_tag="VHS97_04615"
     CDS             3656..>4969
                     /locus_tag="VHS97_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3447509.1"
                     /translation="MRRIKWLSFGLVLAMTSAAVPANPRDGSGHAYDILGDLPVMHRG
                     RVKPLNSMAIEVVKLIYGRSTIELLGRDGKTTSTWEPVAALLDWSARPEFWDNQDFIL
                     VEYLPLKRLLLAASIREQVRSLAGKETPAVKRSLQTLTAQPELTEADLHTAARQAGES
                     STTGKSLNALAAKVGEDHKWLSPRVLENTQLQHEGHSLTFPQWVGEILDKKDRARSGG
                     MGAAPKLTPIEEQAIEIGERFFQYRAIREHNSPAIKPLDLLVVPRPFNKSYGKYSTEV
                     FEKGMEPNQSLSRLEANVANTLVEYLQGLQSKDWALPGEDPVFDQKFEAWLEGSSTWI
                     PLGIILESDELELSRARLPFAQIVAFRKSYRDLEDAERAAPGNAPEASAVAVIAAARD
                     LGTSLAAYPESAAMARESHLNRLAPLSKAPIAHGCGLVLLLLSLGI"
BASE COUNT         1028 a         1461 c         1541 g          939 t
ORIGIN      
        1 gttaatggaa aacgcttcgt cgacattacc gcctcctcag ggaccggcca ccttcaaaaa
       61 ggccacgggg tctcatttgc tgactgggat agtgatggcg atctcgacct ttttgtcgaa
      121 agcggggggg cggtccccgg cgacaaagcc tacaacgtgc ttttccagaa ccccgggcat
      181 ggccggcatt ggctcaaggt aaaactcgtt ggcactaaga cgaaccggtc agccctgggt
      241 gccaggatcc aggtcgacgt cgtgcacaat gacggtacca cccagtcgat ccatcgccag
      301 gtcggaggcg gctccagcta cggaggcaac agcctggtcg agtcgatcgg cctggcagac
      361 gcccaagccg tcgccgcgct ctcagtcacc tggcccgtga gccggacccg ccaatttttt
      421 cggaacatcg ccgccgatca gacaatcgag gtgatcgaag gcgacgatct cccccgcttc
      481 ctccacacgt cgccgatcgt gcaccggcag ccgtgagaac cggcaacccc gaatccggcc
      541 tcattttagt ccgtcggcgc tggctcgacg taacaacgaa gtggaccctt ggacgattta
      601 gacctcgggt cgggtccata ggcgatgacc tggtcgcgct tcagctcggc gagttccttg
      661 tgggtcgtgt acacgatggc acgccccgtg gtgtgaatcc gcaaagtgag ctcctcggcc
      721 ttgggcagag gatgccggaa cagcttgatc agcaactcga tgacgtactg aaaggtatgg
      781 tcttcgtcgt tcaggatcac cacgttgtag ggaggaaggc ggcgagtccg ctggtcggtc
      841 gtcacttcgg tttcgacgga cgtggaggtc gattgttgct cgccgggcat cgcgcgcagc
      901 tcccgcgggg cgattgtacg aattgcgact tacccttagt ctatgatgtt tacctcggga
      961 tgacaaagcg aattccgatc ctggtgtgtt tggtctcttg gatccaagtc gcgagcggga
     1021 acgaattgcg tcggcagatg gcagttgatc cagggcgttg ggtcttctcc cccctggacg
     1081 ttatcgtagg gaggtaccat gaagggcacg agtccagggg ccgcgctacg cgatctgcag
     1141 cgagcccatg ccggtttgaa aaagtcacgc ggaatcttga agctggccaa ggacgatccc
     1201 ggggcaaccg cggccgcatt tgccgcgggc tgggaaagcc tgacccaggc gcaccggatc
     1261 atggcggcaa tttccgccga ggtcgtagac gacgccgtgc tgaccaagca gcttgcctta
     1321 cagcgttacg caacgtcact tctggtgagg ctgcggcggc tcaagcgtag cggcagcgcc
     1381 gggggagtgg cggctgatga cgatgacagc attgatgacg aagacggcga ggattgaaac
     1441 ggaatggcgc gtctgaagaa gctccgtagt ccggttgacg ccgcaccgcg gatgcccgac
     1501 cgcagcctga ccgcggacga acctttaacg acggtggcta tccgcgccgc cggaacccac
     1561 cagttcgtct atcgcaagat ggtcgaaggc cctgtcggcc cggccgctcc caaccacgga
     1621 gacctcgtgc gcgtggtcga ccgcgacggc cagaggctcg gctacgccct ttggaacggc
     1681 cggtcgcaga tcagcctgag gtttctctcg cgccaggaaa acccaccagg agtggagttt
     1741 tggagtcggc gcatcgatcg agcggtcgca ctgcggacca aggtcttgaa ccttgacagc
     1801 cagacgagcg cctaccgtgt cgtccacgca gagggtgacg ggctctcggg actggtggtc
     1861 gatcgctttg acgacgtcct ttcagtccag tgcttcagtc tgggaatgta tcagcggatt
     1921 ggacccatcc tggctctctg cgctgaaaag cttggtaccg cgcattttcg ggtgagcgtc
     1981 gacgagcgcg tggcgatgca ggaagacttt tccggtcggc agctcgtaag cccccaactc
     2041 ccccctcgcg taaccatcat cgagaacggg atccgctatc gcatccactt cgagcatggg
     2101 cataagacgg gctttttctg cgatcagcgc gacaatcgcc gcgacctggc tcggttctcc
     2161 gccgacaaat ccgtacttga tctttgttgc tatacgggag gattcggctt gaatgccctg
     2221 gtccggggga atgcacgcga ggtaacttgc gttgatctcg acgaaaaggc ggttgctctg
     2281 gcgcgtgaga atggccatgc caaccaagtc cggctcgagg tcgtccacgc cgacgcgttc
     2341 ggctacgcac ggcagatggg cttgaacggc aaaacgtttg gcgtggttgt gctcgacccg
     2401 cccaagctta tccccgaccg cgaagagatt gccccgggca agcgaaagta tttcgacttg
     2461 aacgtcctgg cgatgaagct ggtcgagccg ggagggttac tggtgacttg ctcttgctcg
     2521 gggcttttag acgttcagga gtttttggcc ctgctgggag ctgcggctcg aaatgccgag
     2581 aggcacgttc agcttctctc attggtcggc gcggcggccg atcatcccgt cgccatgagc
     2641 gcccttgaag ggagctacct caaagtcgcc tggctgcgcg tggacgaaca accgtagacc
     2701 tccatgaaga tggaaggatt tcgtttcctc gattcagaaa aggacccgaa aatgaaattt
     2761 gcgaatgtgc ttctgttgtt ggcctcggca ggagtattag tcgccgacga gccgaagaaa
     2821 gacgacgccg agtcgatcaa gggaaactgg acagccgtct cgattaagca gggcaaacag
     2881 agtgcgcccg acgattttgt gaagagcttc aagttcggat tggacggcaa gaactacaca
     2941 aatacgatcg gcacccaggt catggaggag ggtggctaca caatcgacgc gtccaagacg
     3001 cccaaaacga tcgactttga catcaagaag ggaccgggcg cgggtaagaa gcagctcgga
     3061 ctctacaaga tcgaaggaga gaaactcacg atcgttgcct ctctggcagg ctcggcggaa
     3121 cggcccaagt ctttggacgc cggagacgcg gatgagttgg cagtggtggt cctcaagcga
     3181 gtcaaaccgt gacgcttcta tcggcgaggc gtgtggcgtt ctaatcccgt tcctcgatca
     3241 aacagtcacc accaaagtcg actggaccag caagcaagaa attaggtcga cgatcacgga
     3301 gaaaagcagc tcgtggatga actgcttctc ctcgacgacg tcgaagcgca gatcgaggat
     3361 ctcgggggtt cagagcgttc tccatgatca ctcgagccat gacggacatg ggatactttt
     3421 gggaaaaacg ctcaatggcg tcgtcgagga tcattgctgc tgcctccgcg gagaatcagt
     3481 aacgcgttct tttggcgaca gttagcagat tcaggtagac gactgcaaag ggctgatcca
     3541 tactagccca accggaaaga cgttcctgct tcgagccgag cagaacagac gcgcttgccc
     3601 gcgcgatcct gctcaaccac tcctcgggtc tcgtcaacaa cccgctccag agactatgcg
     3661 acgaatcaaa tggctctcgt tcggcctcgt cttggcgatg acctcggccg cggtgccggc
     3721 gaaccctcga gacggctccg gacacgctta cgacatactc ggcgacctgc ccgtaatgca
     3781 tcggggccgc gtgaaacccc tcaatagtat ggccatcgaa gtggtcaaac tgatttacgg
     3841 ccgctccacc atcgagctcc tcggccgcga cggtaagaca acgtcgacct gggaacctgt
     3901 ggcggcgctt ctggactggt cggcccggcc tgagttctgg gacaatcagg atttcatact
     3961 ggtcgagtac ctgccgttga aacgtctgct actggcggca tcgatccgtg agcaggtccg
     4021 atcgttggcc ggcaaggaaa cccccgcagt caaaaggtcg ctccaaacgc tgaccgcgca
     4081 gccggagctg accgaggccg acctgcacac ggcggcgcgc caggcgggcg agtcgagcac
     4141 cacggggaag agcctcaacg ccttggccgc caaggtcggc gaggatcaca agtggctctc
     4201 gccgcgcgtg ctcgaaaaca cccagctcca gcacgaaggt cattcgctca cgttcccgca
     4261 gtgggtcggc gagatcctgg acaagaaaga ccgcgcccgt tccggcggga tgggtgccgc
     4321 cccgaaattg acgccgatcg aggagcaggc gatcgagatc ggtgagcgat tctttcagta
     4381 ccgggcaatc cgagagcaca atagccccgc gatcaagccg ctggaccttc tggtggtccc
     4441 gcgacccttc aacaaaagct acggcaagta ttcgaccgag gttttcgaga aaggcatgga
     4501 gcccaatcag tcactgtcgc ggctggaagc caacgtcgcc aacacccttg tggagtacct
     4561 ccagggcctt caaagcaagg actgggccct gccaggggaa gatcccgtgt tcgaccagaa
     4621 attcgaggca tggctggaag ggagttcgac ctggattcca ctgggcatca tcctcgagag
     4681 cgacgagttg gagctgtctc gcgcgcggtt gccgtttgca cagattgtgg cgtttcgcaa
     4741 gagctatcgc gacctcgaag acgcggagcg cgcggcgccc gggaatgcac ccgaggccag
     4801 cgcggtcgcc gtgatcgcgg ccgctcgtga cctcggcacg agcctcgcgg cttaccccga
     4861 atcggccgcg atggcgcgcg agtcgcactt aaatcgatta gcgccgctct ccaaggcgcc
     4921 gatagcccac ggctgcggtc tggtgctact gctgttgagt ctcgggatc
//
LOCUS       DATAQL010000271         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2317657, whole genome shotgun sequence.
ACCESSION   DATAQL010000271 DATAQL010000000
VERSION     DATAQL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317657"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1205
                     /locus_tag="VHS97_04620"
     CDS             285..1205
                     /locus_tag="VHS97_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447510.1"
                     /translation="MRGLVHFFQSEYELAEPVLVESIALARELRDLFSVLVGLMFLGL
                     VQGNRGRISDALATLHEAMKVARRNGDRFFLPRLPNCVGWIYRELQDFDHSREWDRQG
                     LEIAREFDVVEAEANSLISLGSDFANLGEVGKTIPTFREVEACFPSDPWNHWRYDLRL
                     QAAESEHWLRLRDLDQAADHARQLLAAATRHGVSKYIAIAHKLLAEAAMARGDLTDAE
                     VNLAAALDQLHRYPAPLVSWKTYVVLGRLRRQMGDGESAREAFVQAGAIVGEIAANVR
                     DEGLRATFLNSTAVREVLDGAKASSPAAES"
BASE COUNT          343 a          617 c          602 g          353 t
ORIGIN      
        1 cgcgggtgct ttggatcaag cggcgtagga tctcatcaag aggcccgcca aaagagctgc
       61 taatacaccc cccggaccgt tgctcaggga agctccgccc cagcaagaag catcacgaga
      121 ctatatagcc caacatcgcc acatgcccca tcgcctcagg cgtagagtta tggaagtgct
      181 aacgcctgag cagcgatctc gccgcgatca atctgatcct ggatgagatc gttcagaccg
      241 ctcgggcaaa caaccacaag ccgccgcttt gtgtcgggct ccacatgcgc gggttggtgc
      301 acttcttcca gtccgagtac gagctggccg agcctgtgtt ggtcgaatcc atcgccctcg
      361 ctcgggagct acgcgacttg ttcagtgtcc tggtcggcct catgttcttg gggcttgtgc
      421 agggtaaccg tggtcggatc tcggacgccc tggcgacgct gcatgaggcc atgaaagtgg
      481 cccgacgcaa cggcgaccgc ttctttctcc cgcgcctgcc caactgcgtc gggtggatct
      541 atcgcgagct gcaagacttc gatcactcgc gcgagtggga tcggcagggc ctggaaatcg
      601 cgcgggagtt cgacgtcgtc gaagccgagg ccaattcgct catcagtctg ggcagcgact
      661 tcgcaaacct gggcgaagtc gggaaaacga ttccgacctt ccgcgaagtc gaggcttgct
      721 tccctagcga tccctggaac cattggcggt acgacctccg cctccaggcc gccgagtccg
      781 agcactggct ccgcctaagg gacctcgacc aagcggccga tcatgcgcga cagctcctcg
      841 cggccgccac gcgccacggg gtgtcgaaat acatcgcgat cgcgcacaag ttgctcgccg
      901 aggccgccat ggcgcgcggt gacctgaccg atgccgaggt caacctggcc gccgcgctgg
      961 atcaactgca ccgctatccc gcgcccctgg tctcctggaa gacttacgtg gtcctcggcc
     1021 gattgcggcg gcagatgggc gacggggaat cggcgcgaga agccttcgtc caggccgggg
     1081 ccatcgtcgg cgagatcgcc gcgaacgtcc gggacgaggg gctccgcgcc accttcttga
     1141 actccaccgc cgtccgggaa gtcctcgacg gagcgaaggc gtcctccccg gctgcggagt
     1201 cgtgatcgaa ccaccgcctt ggccatcgag tcgttctcag aatctagctt acggtatcgt
     1261 ttcacccgat cggtgaacgg ccctcgacgt ttggtttcgg atgaagcgtg tcaccgcggc
     1321 cgatggacga gccctcgaac agcgagaaat gttcgacgcg tgtcggggtt atgtgggcag
     1381 cctgccgggc gtcgaaacgt cgtcggcaaa ccagtccggg gtggagcctg gatcggattg
     1441 ttccacgagg cagcatactg aacgaaagca acggtaaaga tcgttggttt tttctcagtt
     1501 gcttatcgga acccctttta tcccagcctc gctagaggca tgcagccccg ccgccgtcgg
     1561 ggaggtgccc ggcttcgccg ccgcccccgc aacccggccc ccctggaggg cgaggcctag
     1621 gtgagccatt gcacgtcgga actggggatg atcgcccgcg atcggctcgc tctgctcggc
     1681 caccaggcgg ctcgggaaac tgaggtttcg ttgctcttcc gctccccaag gaggaccagg
     1741 tccggcaccc tggcttgcgc gcgtcggtcc gacgacacga atggcactag cttaagggca
     1801 cgcgaggggc tggcaccgtg gtgaaattgt cgcgcgcacc tggctgaact ttgccacgta
     1861 gaatggaggt aggaggcgat tcctgctcgc gtggcctttc tccggaacgg actcc
//
LOCUS       DATAQL010000272        12571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1096246, whole genome shotgun sequence.
ACCESSION   DATAQL010000272 DATAQL010000000
VERSION     DATAQL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12571
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096246"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1153)
                     /gene="mtnA"
                     /locus_tag="VHS97_04625"
     CDS             complement(83..1153)
                     /gene="mtnA"
                     /locus_tag="VHS97_04625"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX3447511.1"
                     /translation="MISRDGSGPCPTVQWIGDAATGFLRLIDQTQLPAEFVEIDCRDV
                     PAVWEAIKSLRVRGAPAIGIAAAYGAVIGARSRGTPDAGSIRSALGGATQYLRTSRPT
                     AVNLFWALDRMDASAAGVHHDDGPALLGRLLLEARQIEEEDRAMCRAIGRHGAQLVRP
                     GQGILTHCNAGGLATADYGTALAVVFAAHDDGIPLHVFADETRPLLQGARLTAWELTR
                     RGIPVTLICDSMAAQVMRERKVHLVVVGADRIASNGDVANKIGTYGVAVLAKAHGIPF
                     YVAAPSSTFDLSIPDGFAIPIEQRDPREITHGFGRQTAPDGVGVYNPAFDVTPAELIA
                     GIITEKGIINPVNSETIAATLT"
     gene            complement(1241..1573)
                     /locus_tag="VHS97_04630"
     CDS             complement(1241..1573)
                     /locus_tag="VHS97_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447512.1"
                     /translation="MSQDDFDADKTNAHQPEHGHEHEVEEALEEALELDTQSQIFLEM
                     RRQNLDLLRIATQVAGFTGQHGPLKPGDVKNALRNIWDVFSEFYSWIDPEEAEDDESE
                     GDIDEDDE"
     gene            complement(1719..2861)
                     /gene="ald"
                     /locus_tag="VHS97_04635"
     CDS             complement(1719..2861)
                     /gene="ald"
                     /locus_tag="VHS97_04635"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX3447513.1"
                     /translation="MIVGVPKEVKPDEYRVAMLPVGAEELSHAGHTVLIEAGAGQGSG
                     IADASYKAAGATIVSQAAEIWANAELVVKVKEPQPAEWPLLRSGTAVFTYFHFAADEA
                     LTHAIIDSGITAIAYETLRDSRGGLPLLTPMSEVAGRMSIQEGAKYLERPQEGRGILL
                     SGVPGVAPAEVAILGGGVVGSNAAKIAAGLGANVRILDINLDRLRYLDDIMPPNVTTL
                     YSDRHTIVESIERADLVIGAILITGARAPRLVRREDLSQMKNGSVIVDVAIDQGGCVA
                     TSRPTTHREPTYLVDGVVHYCVTNMPGAVGRTSTYALGNVTLPYVLQLVQHGWRNLAA
                     ADSGVADGVNIDQGRVTNRAVAATFGLPFHPLDERPASVAMPAARA"
     gene            complement(2923..3840)
                     /locus_tag="VHS97_04640"
     CDS             complement(2923..3840)
                     /locus_tag="VHS97_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3447514.1"
                     /translation="MNPMTRRELFGWTAGAIAAGSASLAGSRRAMAIPPVARSRPSHM
                     KLSLAAYSYRDYLTGKKSPKLDLFDFVNLAADMALDGVEPTSYYFPGDVTSDYLDRLK
                     LHAFSLGLDISGTAVGNNFCLPPGPKRDEQITLVERWIDNAAELDAPIIRIFAGNVAK
                     GSTEEQAVGWAIDGIKTVLPYAAKKGIVLALENHGGITATPAQIMKLVSAIDSPNFGV
                     NLDTGNFRGSDPYAEIAQLAPYAMNVQVKTEIQRGSGHPKEAADLARIVGILRDAHFS
                     GYVVLEYEAADDPMKAIPIHTKTLRKLIS"
     gene            complement(3978..5501)
                     /gene="der"
                     /locus_tag="VHS97_04645"
     CDS             complement(3978..5501)
                     /gene="der"
                     /locus_tag="VHS97_04645"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX3447515.1"
                     /translation="MSLPKVVIVGRPNVGKSSLFNWLAGRRIAIVDSMAGVTRDRVGS
                     LVQLGEDDDPRFIELFDTGGVGMVDRDDLSEDVERQIDTAMNEADLILFVVDIRDELM
                     PLDEEVAGRLRYLKTPVILVMNKADVPEFDHRGADFYKLGRGKPIAVSVHQGRNKKQL
                     LKMIEEMLPAVESVKPANAVMKIAVVGRPNTGKSTFINTLARAERMIVSERPGTTRDS
                     VDVHFELDGMPFLAIDTAGIKRHSKIRDSLDFYSVHRAQRSIRRADVVLLFLDAAQGI
                     SRLDKQLADYIAQEHKPCIFTINKWDLMLTDPADPTQGNMGRYANLVQHAFRSMSYMP
                     MAFITAQTGKNVKALINLSQAIFKQSRKRVGTGTLNRIVREAVDAHPPASRENRNPRI
                     YYATQVGVAPPTIVLFVNSTRLFDPTYQRYLLNVLREKLPYHDIPIKLYMRSRKQNEP
                     GDHSSSDDSEARDDHLDMNPAAARADSPKQSPQHRHLDFSTRFLNREVNELLSELDS"
     gene            5798..6319
                     /locus_tag="VHS97_04650"
     CDS             5798..6319
                     /locus_tag="VHS97_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447516.1"
                     /translation="MEQVDAGDGFSVLDGAALVMGSAIASIHILGVRRWDVSVTGWIM
                     VAITFGWVSLTAAGPFIYVARRYARRLPHYPGIGDRLWAVLGIPWLATALVQSAAPTT
                     EPGHNPLLATTLSLGLATACLIALVVVWSTWVTVPPEQAAQVEAAPWTNRVGLILSIA
                     WPIQFGLGMVVLS"
     gene            6327..6869
                     /locus_tag="VHS97_04655"
     CDS             6327..6869
                     /locus_tag="VHS97_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447517.1"
                     /translation="MRTLWQGSRLRFHLQDFLAVVIGYGMAALLCRAFWPASPPSPAL
                     GVPGIGVYLWLGLAMSGPVILLRRGPPRTDSATAADKPTSSHSRTWAELAWMLIGIYW
                     IVVSLIVIPARLSEFKLGDMILFGLVPFAVALAMRLLGPRPKENLDSARAWTHTVGVA
                     VLLTWPVAWICLIVLGNALP"
     gene            complement(6936..8822)
                     /locus_tag="VHS97_04660"
     CDS             complement(6936..8822)
                     /locus_tag="VHS97_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447518.1"
                     /translation="MPFEVFRRHQRHLIAIFGILAMFGFVVSDSLPKLLNSNTGVQDP
                     PVVKLYGKTVYRSALAGMLEQRTLANNFVSELIPYMPRNPFGGTKDRDLVDALILQHE
                     ADRLGLPGGPEIGREFLKRITGGKMTVELFELLLSRLNNRVSGAQLLADIANQVRLAN
                     VRRLMGEPLVTPYDVFQSYRDQNERVSAKLVEIPVEKFLAQVPEPTPEELKAEFDKYK
                     DVLPDPARETPGFKVPRQIQLEILSIDGNALARGIRDKITEAELRAAYENRKSEFQER
                     SELPNDLFAGQPELTPPVIRPFADVRAELAVALAEERAQAEIIDRFTKLKDEVLFPFF
                     ESYAQAVEEIEEAKKLGTKPKTELPAANDLKELAEREVLGYEVTRLLARDQEDQFGQI
                     ARAEVGIAKPNGGRKFAEEFFDPKKPLYEPEELTDLLGTRYLARKIKDVPAHVPSLED
                     VRSDVSLAWKMSKARTLAENAAQLLAEQLKKKGEVPKETTIDGYRVVSIPAITRRQTS
                     FLPSRFETGPPEESPIPDVTYPGDLFRKSYFGLQPGSVAVASNQPRTVYYVMVPDRRE
                     PATFASLYAPNGDEFRYKMSARQEAGRQLEDQWMGWLRHQAGLDSTWIAPDESRAKSS
                     ADDA"
     gene            9104..11755
                     /gene="topA"
                     /locus_tag="VHS97_04665"
     CDS             9104..11755
                     /gene="topA"
                     /locus_tag="VHS97_04665"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX3447519.1"
                     /translation="MARKSNQSTRPDAGPEPKKPAPKRSATAKTPRPKTAARKKSGNY
                     SEASSNGDLPSLVIVESPKKAKSIHKFLGTTYVVKASMGHVRDLPKRKLGLDVAQGYK
                     ASYEIVPTKKDTIGELKRAAAKAGLVYLATDPDREGEAIAWHLQQALDLTDDRVRRVT
                     FHEITERAVKEAFNRSGPINMDMVNAQQARRFLDRFVGYQLSPLLWKKVGARNLSAGR
                     VQSVAVRLIADRERDIRAFVTEEYWKISATVCPVGSTLATDQFKASLAEYNGAKFEAK
                     NEADAHAVRDALQSVPYAVSKIDETEKLDKADPPFKTSTLQQQAAIRLRSSGKKTMRT
                     AQELYEGIEVDGSGPVGLITYMRTDSLRVSDDAMTAVREKIKNDFGDRYLPAKPIRYA
                     AGKNAQEAHEAIRPTDLALTPEKIKGSLTHDQFRLYQLIYARFVASQMAPAVFMVTDV
                     AITAGLGLFKAQGKVQKFDGHRRVWPPGGKQEDELLPLLTANQALDKKSLEATQHFTQ
                     PPPRYSEATLIKALEKEAIGRPSTYAPIIQTIQDRGYVAQKERRFHATDLGMAVTNLL
                     VEQFPKILDLKFTAQMEDKLDDIAAAKEEMTKVLDEFYYPFQEELKVAETHTERVQIP
                     TGETCHVCAAPMVLKFGKTGQFLGCSKYPECKATRPLGGQPRVEAVESEHSCPKCGKK
                     LLIRQNKRGEKFLSCSGFPECKESFNINPEGNPVPSVVATEHTCEKCGKPMALRQGSR
                     GAFLGCTGYPKCRNALPVDEEGRPVQPVKVDIACTKCGGAMAVRRGRRGSFLGCTNYP
                     KCRGTAQIPDELKEKLAEQPSPAAAPGPDLKNIPIEEMCEQCGGPMLVRRSRRGYFLG
                     CAQYPQCKGTRQPDEATLAKITEVMGA"
     gene            complement(11842..12267)
                     /gene="acpS"
                     /locus_tag="VHS97_04670"
     CDS             complement(11842..12267)
                     /gene="acpS"
                     /locus_tag="VHS97_04670"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX3447520.1"
                     /translation="MEILGIGTDIIECPRIGKMIEHHGELFLRRVYTEREIRYCQARK
                     HAIEHFAGRWAAKEAILKAMGTGRSHGIAWTDVEVRNGHSGQPRVLVCGAAREATRER
                     GIADILVSISHCRTYATAYAMALGRQAPAPKPPDPALPA"
BASE COUNT         2518 a         3747 c         3774 g         2532 t
ORIGIN      
        1 cttccgagcg atcattgagc gaaaagaaaa cgctgtgagc cagcatcggt ctgctccacg
       61 aaaacggaca gaagaggttg gttcaagtaa gggtagccgc gatcgtttcg gaattcaccg
      121 gattgatgat tcccttctcg gtgatgattc ccgcgatcaa ctcggctggc gtgacgtcga
      181 aggcggggtt gtaaacgccg acgccgtcag gcgctgtctg gcgtccgaat ccgtgggtga
      241 tctcgcgcgg gtcgcgctgt tcgatcggga tagcgaagcc gtcggggatc gacaaatcga
      301 aggtactgga cggcgcggcg acgtaaaacg ggatgccgtg cgctttggcc agcacggcaa
      361 ctccgtaagt gccgatcttg ttggctacat cgccgttgga ggcgatacgg tcggccccga
      421 caacgacgag atggaccttt ctttctctca tcacctgagc ggccatgctg tcgcatatga
      481 gcgtcaccgg aatgccccgc cgcgtgagct cccacgcggt gagccgcgct ccttgtaaga
      541 gcggccgggt ttcatcggcg aatacatgca gaggtattcc gtcgtcatgg gcggcaaaaa
      601 cgaccgccag ggccgtgccg tagtcggccg tggccagacc gccggcattg cagtgagtca
      661 agatcccctg ccccggccgc acaagctggg caccatgccg gccgattgcg cgacacatcg
      721 cgcggtcttc ctcctcgatt tgcctggcct ccagcaacag ccgacccaga agcgccgggc
      781 cgtcgtcatg gtggacccca gcggcggaag cgtccatccg gtcgagcgcc caaaacaggt
      841 tgacggcggt cggtcgactc gtacgcagat attgggtagc gccgcccaag gcgctgcgga
      901 tcgatccagc gtcaggtgtg ccgcgcgacc gcgcaccgat caccgcacca tatgccgccg
      961 cgatgccgat cgccggcgcg ccgcggaccc ggagcgactt gatcgcttcc cacactgccg
     1021 gcacgtcgcg acaatcgatt tcgacgaact ccgcgggaag ctgagtctgg tcgataagtc
     1081 gcaggaaacc cgtcgccgcg tcaccaatcc attggacagt tggacacgga ccggatccgt
     1141 cacgggaaat catcgaactt ggtcatcccc cgatgtgaca aagcctccct gcgaccgaga
     1201 cggcccgccg tcatcggttg cggaagcact gaagtcgcgg tcactcatca tcctcgtcaa
     1261 tatcgccttc ggactcgtca tcctccgctt cctcaggatc aatccaggaa tagaattccg
     1321 agaagacgtc ccatatgttg cgcagcgcgt tcttgacgtc acctggcttc aagggcccat
     1381 gttgacccgt gaagccggcg acctgggtgg caatccgcag cagatcgagg ttttggcgcc
     1441 tcatctccag gaagatctgg ctctgcgtgt cgagctcgag tgcttcttcg agagcttcct
     1501 cgacctcatg ctcgtgaccg tgctcgggct gatgtgcgtt tgtcttgtcg gcatcaaagt
     1561 cgtcttggct catcggttag agttctcccc cataagtcgg tttaagaatt gtgctcgacc
     1621 tatccaaccg ccgaaacata aaaacgaaga atgaatcgga cactgtggtg gccaccactt
     1681 attctggtca tattggcctt cggatgtcac gaacatggtc acgcgcgcgc agccggcatg
     1741 gccaccgatg ccggtcgctc gtcgagcggg tggaatggta aaccaaaggt cgcggccacg
     1801 gcgcggtttg tcacacggcc ctgatcgatg ttaaccccgt ccgccacgcc ggagtcggcc
     1861 gcggcgaggt ttcgccagcc atgttgcacg agttgcagca catacggcag tgtgacattg
     1921 ccaagtgcgt aggtgctggt tcgacccacg gcgccgggca tgttcgtgac gcagtagtgg
     1981 acgactccgt cgacgagata tgtgggttct cgatgcgtcg tcggccggct ggtcgcgacg
     2041 catcctccct gatcgatggc gacatccaca atcacggagc cgttcttcat ctgcgacagg
     2101 tcttcgcgcc tgaccagacg gggcgcgcgc gctccggtga tcaggatcgc gccaatgacc
     2161 aggtcggcgc gctcgatcga ttcaacgatg gtatgtcgat ccgagtagag cgtggtgacg
     2221 ttgggcggca tgatgtcatc cagatagcgc aagcgatcga gattgatgtc aaggatgcgc
     2281 acgttggcgc cgaggccggc ggcgatcttg gccgcgttgg agccgacaac acccccgccg
     2341 aggatcgcga cttcagccgg ggcgactcca ggcactccgg aaagcaagat tccgcggccc
     2401 tcctgaggac gttcgaggta tttggctccc tcttggatgc tcattcgccc ggccacttcg
     2461 ctcattggtg tcaagagagg aaggccgccg cgcgaatcgc gcagtgtttc gtatgcaatc
     2521 gccgtgattc cgctgtcaat gatggcgtgg gtgagtgcct catcggcggc aaaatgaaag
     2581 taggtaaaga cggctgtacc tgaccgtagc aacggccatt ccgcaggctg aggttccttg
     2641 accttcacca cgagctcggc attggcccag atctcggctg cttgagacac gatggtcgcc
     2701 ccggcggcct tataagacgc gtcggcgata ccgctcccct gccccgcgcc ggcctcgatc
     2761 aagaccgtgt ggcccgcgtg gctcaactct tcggctccga ccgggagcat cgccacgcga
     2821 tactcgtccg gcttgacctc tttgggcact ccgacaatca tttcgaacgt ctccaaggac
     2881 cggccgacga tcctggcgac gtcgggttta tccaagctgc catcaactga tcagtttccg
     2941 caacgttttc gtatgaatgg gaatcgcctt catcgggtcg tcggccgcct cgtactcgag
     3001 gacgacatag cccgagaaat gtgcatcgcg taagataccg acgatccgcg ccaggtcggc
     3061 cgcttccttg ggatgaccgc tgccgcgctg gatttcggtc ttgacctgga cgttcatggc
     3121 gtagggcgcc aattgagcaa tttcggcata agggtcggag ccgcggaagt tgcccgtgtc
     3181 gaggttcacg ccgaagttgg gtgagtcaat tgcgctcacc agtttcatga tctgtgcggg
     3241 tgtcgcagtg atcccgccgt gattttcgag agccagcacg atcccctttt tggcggcata
     3301 tgggaggacg gttttgatac cgtcgatggc ccagccaacc gcctgttcct cggtcgatcc
     3361 cttggcgacg ttgccggcga aaattcggat gatcggggca tccagctcag ccgcgttgtc
     3421 gatccaacgc tcgacgagcg ttatttgctc gtcgcgtttc ggaccgggtg gaaggcaaaa
     3481 gttgttgccc accgcagttc ccgagatatc cagaccaagc gagaaggcgt gcagtttgag
     3541 tcgatcgaga taatcggacg taacatcgcc tggaaagtag tatgacgttg gttcgacgcc
     3601 gtcgagcgcc atgtccgcgg ccagattgac gaagtcgaat agatcaagtt tggggctctt
     3661 tttgcctgtc agataatcgc ggtatgaata ggcggccaaa ctgagcttca tgtggctggg
     3721 ccgactgcgt gcgaccggcg gaatcgccat ggcgcgccga ctgcctgcga ggcttgccga
     3781 tccggccgca atggctccgg cggtccaccc gaataactcg cgccgcgtca tcggattcat
     3841 ggtgttctct cccggatgaa aatgacagcc agtgaggcct tcgcctcggt cttctaggcc
     3901 aaagagcacg gcccatcgcc ccagagatct tatctcagtg agcgttgtgc ttccaagcaa
     3961 caagtggcgg caatttttca gctatccagc tcggagagca attcattgac ttcgcggttc
     4021 aaaaaccgag tcgagaagtc gagatgcctg tgctggggcg actgtttggg actgtcggcg
     4081 cgagcagccg ccggattcat atcgagatgg tcgtcgcgtg cttcggaatc atcggaagag
     4141 gagtggtcgc cgggctcgtt ctgcttacgg gaacgcatgt agagcttgat cgggatgtcg
     4201 tggtacggca gcttttcgcg gagcacattg agcaggtagc gttgataggt gggatcgaaa
     4261 agccgtgtgc tgttcacgaa gagtacgatc gtggggggtg ccacgccgac ctgagtggcg
     4321 taatagatcc gtggattgcg gttctcgcgt gaagcgggcg ggtgcgcgtc gaccgcttcg
     4381 cggacaatcc ggttgagcgt gcccgtgccc acgcgcttcc gcgactgctt gaagattgct
     4441 tgcgagaggt tgatgagcgc cttcacgttt ttgcccgtct gggccgtgat gaaggccatc
     4501 ggcatatagc tcatggagcg gaacgcgtgc tggaccaggt tggcgtaccg gcccatgttt
     4561 ccctgggtgg ggtcggcggg atccgtgagc atgaggtccc atttattgat cgtgaagata
     4621 cagggcttgt gctcctgggc gatgtaatcg gccagttgct tgtcgagtcg gctgatgcct
     4681 tgcgccgcgt caagaaacag taaaacgacg tcagcgcgac gaattgatcg ttgagcgcgg
     4741 tgtaccgaat aaaagtcaag gctgtcgcgt atcttggagt gccgcttgat tccggccgtg
     4801 tcgatcgcca ggaacggcat gccatcaagc tcgaaatgaa catccaccga atctcgcgtt
     4861 gttccgggac gttccgagac gatcatccgt tctgcgcgtg ccagcgtgtt gataaaggtc
     4921 gacttcccgg tgttgggcct gccgactacg gcaatcttca tgacggcatt ggccggtttg
     4981 accgattcga cggcgggaag catttcctcg atcatcttga gcaactgctt cttgttgcgc
     5041 ccctggtgaa cggagacggc aatcggtttg ccgcggccga gcttgtaaaa gtccgcaccc
     5101 cgatggtcaa actcggggac gtctgccttg ttcatcacca gaatgaccgg agtcttcaag
     5161 tagcggagcc gaccggcaac ttcttcgtcc agcggcatca attcgtcgcg aatgtcgacg
     5221 acaaagagaa tcaggtcggc ctcattcatc gccgtatcga tctggcgctc gacgtcctcg
     5281 ctcagatcgt cgcggtcgac catgccgacc ccaccggtgt cgaagagctc gatgaatcgc
     5341 ggatcgtcgt cctcgcccag ttgaacgagc gagcccacgc ggtcccgagt cacgcccgcc
     5401 atcgagtcga caatggcaat tctgcggccc gccagccaat tgaacaacga cgacttgccg
     5461 acattcggcc gtcccacaat aactaccttg gggagtgaca ttttgaccac caacaagacg
     5521 gcgcggagag gtgccggaat accggcaaaa tgagttagcg tgggcaagag gcgacaaagc
     5581 aaatccaatc actcaatgcc gaccgattcc ctgatatctc cgcgaccatc gaaaaacagg
     5641 aatgaaaagc ggattacgca ttattatacg accgtcgacg acgtctcggc aaattatgct
     5701 cgaaccgcgc cacgatgaaa tacttgtttg cgcggattac aatacctgaa tccacgtcgg
     5761 ttccttcgga aaggcggaag tgggagattg gcggagaatg gagcaggttg atgccgggga
     5821 tggcttcagt gtcttggatg gggcagcgct cgtcatgggg tccgcgatcg cgtcgattca
     5881 tattctcggc gtgcgacgat gggacgtttc cgtgacaggc tggatcatgg ttgcgatcac
     5941 ctttgggtgg gtgagtctga cggccgccgg gccgtttatc tatgtggcgc ggcgatatgc
     6001 caggcgactg ccacactatc ccggcatcgg cgaccgcctc tgggcggtgc ttggtatacc
     6061 gtggctggcg actgctttgg tacaatcggc cgcgcccacc actgagcctg gccacaatcc
     6121 tcttttggca acaacattga gccttgggct ggcaacagct tgtctgatcg cgctcgtggt
     6181 cgtctggagc acgtgggtga cagtcccgcc ggagcaggcc gcgcaagtag aggcggctcc
     6241 ctggaccaat cgcgtcggat tgatcctatc gattgcctgg cccatacagt ttggcctggg
     6301 gatggtcgtc ttgagctaac cggaacgtgc gcactctctg gcagggatct cgccttcggt
     6361 ttcatctcca ggattttctg gcggttgtga tcggttacgg aatggccgcg ctgctttgtc
     6421 gcgcattttg gccggcgagc cctccgtcgc ctgccttagg ggttcctggg attggcgttt
     6481 acctttggct tggtctggcg atgagcgggc cggtcatcct gttgagacgt ggtccgcccc
     6541 ggacagactc cgcaacagcg gcggataagc ccacgtcgtc tcattcacgc acctgggcag
     6601 agctagcctg gatgctgatc gggatttact ggatcgtggt gagcttgatc gtcataccgg
     6661 cgcgccttag cgaattcaaa ctgggcgata tgatcttgtt cggcttagtc ccgtttgcgg
     6721 tcgcgctcgc gatgcgtctg ctcgggcccc gaccaaaaga gaatctagat tcggcgcgtg
     6781 catggaccca caccgtgggc gtagcagttt tgttgacctg gcccgtcgcc tggatttgtt
     6841 tgatcgtgtt gggaaatgcg ctgccgtaag cctcgacgcg atcgcaggac ctcagccctc
     6901 gaattcgaga ttgtagacgg ctattcagcg acagctcagg cgtcgtcggc cgacgacttg
     6961 gccctggact cgtcaggcgc aatccaagtg ctgtctagac ctgcttggtg gcggagccag
     7021 cccatccatt ggtcctcgag ctgtcggccg gcctcttgcc gtgcggacat tttataccgg
     7081 aattcgtcac cgttcggcgc gtagagtgag gcgaacgtgg ccggctcgcg acggtcgggg
     7141 accatcacat aatagacagt gcgaggttgg ttggacgcaa cagccaccga gcccggctga
     7201 aggccgaagt aagattttcg gaacaaatcg cccgggtagg tgacgtctgg tatcggactc
     7261 tcttccggtg gcccagtttc gaatcggctg ggtagaaaac ttgtttgccg acgcgtgatc
     7321 gcgggaatcg agacgactcg gtacccgtcg attgtcgttt ccttggggac ttctcccttt
     7381 ttcttgagct gttcggccaa caactgcgcg gcattttcgg cgagagtacg cgccttggac
     7441 attttccagg cgaggctgac atcggagcgc acgtcttcga gcgacggaac gtgagccggg
     7501 acatctttga ttttgcgggc aaggtaacga gtcccaagta agtccgtcaa ctcttccggt
     7561 tcataaagcg gcttcttggg atcgaaaaat tcttccgcga actttcgacc tccgttcggc
     7621 ttggcaattc cgacctccgc tcgcgcgatc tggccgaact gatcttcttg gtcgcgagcg
     7681 aggagacggg tcacttcata gcccaagacc tcgcgctcgg caagctcttt gaggtcgttg
     7741 gcagcgggca attcggtttt gggcttggtc cccagtttct tggcttcttc gatctcctcg
     7801 acggcctgcg catagctctc gaagaacggg aacaagacct catccttgag cttggtaaat
     7861 ctgtcgataa tctcggcctg cgctctttct tccgccaagg ctacggccaa ctcggctcgt
     7921 acgtctgcaa atggtcttat cactgggggc gtcaactcgg gctgaccggc gaagaggtcg
     7981 ttgggtagtt cggaccgttc ctgaaattcc gacttgcgat tctcgtaggc ggcccgtaac
     8041 tcggcctctg tgatcttatc cctgattccc cgcgcaagcg cgttgccatc gatcgaaagg
     8101 atctcgagct ggatctgccg gggtaccttg aacccagggg tctcacgcgc cggatcggga
     8161 agaacgtctt tgtatttgtc gaattcggcc ttgagctctt cgggggtcgg ctcggggacc
     8221 tgcgccagga acttttcgac ggggatttcc accagcttgg ccgagacgcg ctcattctgg
     8281 tcgcggtacg actggaagac gtcataggga gttaccagtg gctcgcccat gagccggcgc
     8341 acgttcgcga gacggacctg attggcaatg tcagccagga gttgggcacc gcttacccga
     8401 ttattgagac gactcaagag caactcgaac aattccacag tcatcttgcc accggtgatt
     8461 cgcttcaaga actcgcgacc aatctcagga ccacccggca gacccagtcg atcggcttca
     8521 tgctgaagta tcagggcgtc tactaaatct ctgtccttgg tgccgccaaa gggattgcgc
     8581 ggcatatagg gaatgagctc ggacacgaag ttgttggcta gcgtgcgctg ctcgagcatt
     8641 cccgcaagcg cgctgcgata gaccgtcttg ccgtacaact tgacgacggg cggatcttgc
     8701 acgcccgtgt ttgaattgag cagcttgggc agactatccg agaccacaaa gccgaacatc
     8761 gccagaattc caaaaatggc gataagatgc cgttgatggc gtctgaagac ctcaaaaggc
     8821 atggagttcc tcgagtttca atattccaat tggccggcgc ctggaggtcg aaataagggg
     8881 ttaaagccgg gcgagttgcg tcaccacccc ttgacatacc ccgatacgct agcttatgtc
     8941 gcttagggaa aggatgcggg gtttgacccg aacataacct ctccatcatc gtaccaggat
     9001 ttgacaagaa gacaatctca atcacgcgtt cgatgccgtt cgcacgactt gggaaggatc
     9061 gacccaagcg gtcatctgag aacaggatag gtaggacagc actgtggctc gcaagtccaa
     9121 tcaatcgact cgtcctgatg cgggaccgga acccaaaaag ccggcgccaa aacgatcggc
     9181 gactgccaag actccgcgcc cgaaaaccgc ggcccggaaa aaatctggga attattccga
     9241 agcatcgtcc aatggcgacc tcccctccct ggtgatcgtc gagagcccga agaaggccaa
     9301 gtcaattcac aagtttctcg gcacgaccta cgtggtcaag gccagcatgg ggcatgtgcg
     9361 cgatctgccg aagcgcaagc tggggctcga cgttgcccag ggttacaagg cgtcttacga
     9421 aattgtgccg accaagaaag atacgatcgg cgagctcaag cgagccgctg cgaaggccgg
     9481 tcttgtctac ctggcgaccg accctgaccg cgaaggtgag gcgatcgcct ggcacctgca
     9541 acaagcccta gacctcactg acgaccgcgt tcgccgcgtc acgtttcatg aaatcaccga
     9601 gcgcgcagtg aaggaggcgt tcaaccggtc tggcccgatc aacatggata tggtcaatgc
     9661 ccagcaggcc aggcggttcc tcgatcgatt tgtcggttat cagttgagcc cactcttatg
     9721 gaagaaagtc ggtgctcgca acctgagcgc cggccgcgtt cagtcggtgg cggtgcggtt
     9781 gatcgccgac cgcgagcgcg acatccgggc cttcgtcacc gaggaatatt ggaagatctc
     9841 ggcgacagtc tgccccgtcg gctcgacact cgccaccgat caattcaagg cgagcctggc
     9901 cgagtacaac ggagccaagt ttgaagccaa aaacgaggcg gatgcccacg ccgtccgcga
     9961 tgcactccag tcggttccct acgccgtttc caagatcgac gaaactgaaa agctcgacaa
    10021 ggccgaccct cccttcaaga cgagtacgct ccagcagcag gccgccatcc ggcttcggtc
    10081 gtcgggcaag aagaccatga ggactgccca ggagctctac gagggtatcg aggtcgacgg
    10141 ttccggtccg gtcggtctga tcacctacat gcgtacggat agccttcgtg tctccgacga
    10201 tgccatgacc gcggtacgcg aaaagatcaa aaacgatttc ggcgatcgct acctccctgc
    10261 caagccgatc cgctacgccg ccggcaagaa tgcccaggag gcccacgaag ccattcgccc
    10321 gaccgacctg gccctcacac cggaaaagat caagggctca ctcacccatg accagttccg
    10381 gctctaccag ttgatctacg cgcggttcgt cgccagccag atggcccccg cggtcttcat
    10441 ggtgacggac gtcgcgatca ctgccggcct gggactgttc aaggcgcagg gcaaggtaca
    10501 gaagttcgac gggcaccggc gggtatggcc ccccggcggc aagcaagaag acgagttgct
    10561 gccgctattg accgccaatc aggcactcga caaaaagagc ctggaggcaa cccagcattt
    10621 cacccagccg ccgccccgct acagcgaggc cacactgatc aaggccctcg aaaaagaggc
    10681 aatcggccgg cccagcacct atgcgcccat catccagacg atccaggacc gcggatatgt
    10741 cgcgcaaaaa gaacggcgct tccacgcgac cgacctgggg atggccgtga ccaaccttct
    10801 cgtcgagcaa tttcccaaaa tcctcgattt gaagttcacc gcccagatgg aagataagct
    10861 cgacgacatc gccgcggcca aggaagagat gacgaaggtc ttggacgagt tttactatcc
    10921 atttcaggaa gagctgaaag tcgccgaaac gcacacggag cgggtccaga ttccgaccgg
    10981 cgaaacttgt cacgtctgcg ccgcgcccat ggtgctcaag ttcggcaaga ccggccagtt
    11041 cctgggctgc tccaaatacc ccgagtgcaa ggcgacgcgc cccctcggag gccagccccg
    11101 cgtcgaagcc gtcgagagcg agcattcctg tcccaagtgc ggcaagaagc tattgatccg
    11161 ccaaaacaaa agaggtgaaa agttcctgtc gtgctccgga ttccccgagt gcaaggaatc
    11221 gttcaacatc aacccggaag gcaatccggt cccctctgtc gtcgcgaccg aacacacctg
    11281 cgagaaatgc ggcaaaccga tggccttgcg ccagggatcg cggggcgcct tcctgggctg
    11341 cacgggctac ccgaagtgcc gcaacgcact gccggtcgac gaagagggcc gccccgttca
    11401 gcccgtcaag gtcgacatcg cctgcaccaa atgcggtggt gccatggccg tgcggcgcgg
    11461 acggcgcggt tcattcctcg gctgcacaaa ctatcccaag tgccggggca cagcccagat
    11521 acctgacgag ctcaaagaaa agctcgccga gcaaccgagc cccgcggccg ccccgggccc
    11581 cgacctgaag aatatcccca tcgaggaaat gtgcgagcag tgcggcggcc ccatgctcgt
    11641 gcgtcgcagc cgccgcggat attttctcgg ttgtgcccag tacccccaat gcaaaggaac
    11701 gcgccagccc gacgaggcga cgctcgcgaa aatcaccgag gtgatgggag cgtagccgac
    11761 cgacccgccg cgcggactca tcaaaatcct gaagcgcgga ccggcctggc gccgatcgta
    11821 tccactcgcg aaacgaattc gtcacgcggg aagagccggg tcaggcggct tgggcgcggg
    11881 agcctgccga cccaaagcca tcgcatacgc ggtggcgtag gtccggcagt gagaaatgga
    11941 aaccaggatg tcggcgatac cacgctcgcg cgtggcctct ctggccgctc cgcagacgag
    12001 gacccgcggc tgcccactat ggccattgcg gacctcgacg tcggtccagg cgatcccatg
    12061 gctgcggccc gttcccatcg ctttcaagat cgcttccttg gcagcccacc ggccggcgaa
    12121 gtgctcgatg gcgtgtttgc gcgcctggca gtagcggatc tcccgctccg tgtaaacgcg
    12181 ccgcaggaat agctcgccat ggtgctcgat catcttgcca atgcgcgggc actcgatgat
    12241 atcggtgccg attcccaaga tttccactcc tgtccccttc ccttggccga gccggtcgaa
    12301 ttgacctcgt gataggcaga tcgccaatgg ttcagtgcgg catggcgttc cgggccgcgg
    12361 ttcaccacgg gcaagatgcc cgtgccacac cacactgcgg gctgacgcag tgcgagtctc
    12421 gacccaatct aattccgaag gactcaacgg ccaagtgctc aatcaatgac gaagcttcaa
    12481 tattcagccc tcttcgcgct ctatcgacga cctacgcagg acgaaggtcg tgcggcggcc
    12541 gaagtcgtgt cgcggcccat ggcctgcgcc g
//
LOCUS       DATAQL010000273         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4606869, whole genome shotgun sequence.
ACCESSION   DATAQL010000273 DATAQL010000000
VERSION     DATAQL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4606869"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VHS97_04675"
     CDS             complement(<1..230)
                     /locus_tag="VHS97_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3447521.1"
                     /translation="MAGASLAGKPRKVIPKILAVIHADGCTGCEACIEVCPVDCIYKV
                     PGEDYACLQTWCDIELDRCIGCKHCAQVCPWDA"
     gene            690..2210
                     /locus_tag="VHS97_04680"
     CDS             690..2210
                     /locus_tag="VHS97_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HEX3447522.1"
                     /translation="MPKKVAVFWPGDYRSKPNEWALSQSREATSQLVAALKKLGRSPY
                     LVEGYLTRPDQAIAKLGPIDDPLVGLFVHWTYAPHTVDGVVGKDSPLLLASNFSGTWP
                     GLVALLNTGASLECVGRPASRIWTDAPEWTADNLFMERLDQWCTSGGIEYPMTELRGA
                     GAISPESNALAAQVEASIRRKRILALMLGDTSMGMINGYFGPRTLYPIGFSEHKVDQA
                     WLIERTKAISQSRVDDAFRFVKDRGVTFHWKEEDALDFTEAATREQLRGYLAVLDLIS
                     EFGADCVGWQYQLGLLNLLPPSDFAEGLLNSHARPEGNGHVMITSTEADQGNLVPMEL
                     MKRVLEAKGLPGAVMFHDVRWGARHDGRFLWVLLNSGSCGAYAFNQDVDSLAGTHSFR
                     QPAGYFPVPGGTFAGVSMPGPITWTRSWIDRTGQLVMDLGRGESVALPADIREAWWRG
                     TTRQWPFMAADLGCSMETIMAHYMSNHIAVAYGDIFGEMAALSQSLGFKVRVLASS"
     gene            2537..>2853
                     /locus_tag="VHS97_04685"
     CDS             2537..>2853
                     /locus_tag="VHS97_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447523.1"
                     /translation="MAASLLWLVWVTDRALGQSPTGQTQSHASFSAQQSLLNKTRQPS
                     HQTIARTVSARPRADQPPPLPPAAVVSSPATEPSAPPPKRFKVRDEFGNCVVARFHGE
                     FGG"
BASE COUNT          574 a          915 c          812 g          552 t
ORIGIN      
        1 gcatcccacg gacacacttg agcgcaatgc ttgcagccga tgcatcgatc aagttcgatg
       61 tcgcaccacg tctgcaaaca ggcataatct tccccgggca ccttgtagat gcagtcgacc
      121 gggcagacct caatgcacgc ctcgcagccc gtacaaccat cggcgtgaat gaccgcaaga
      181 atcttgggaa tgaccttacg cggtttacca gccagggacg ctcctgccat gtgacgcctt
      241 ccccgatcta gatctatatg acccgtcatc ccgcctggag agcgagtgca tgtcgaagtc
      301 gaaacagccg tcaggatcga cacagatcga ccgcacaagc cgccgagcga cacccaggca
      361 gcatcgtccg tctgtgacac taagaatcat tatcgttatt cagaatgata gggtcaagct
      421 tgtcattgaa atcacgccag aggcatgcgc cgccgccacg agatcgatgc cgaccgattc
      481 gcacggcggg acgagcctaa aaaatcgcaa gaccagcaat cctcgtgagc ggtcgtgcgt
      541 cgtcggcttg cgatctcttt aaccctctgt ttgctcgcga tccgaccgcc acaacactcg
      601 tggtccgctg tagatcggcg tgttccggcg atatacactc tggtctaatc gatgaccact
      661 ctccaccgta gacgaattca gagatttcca tgcccaaaaa agtcgctgtt ttctggcccg
      721 gggactatcg gtccaaaccc aatgagtggg ccctgtcgca atcacgcgaa gccaccagcc
      781 aactggtcgc ggctctcaaa aaactgggac gatcgccgta tctcgtggag ggatatctaa
      841 ctcgtcccga ccaggcgatc gccaagctgg gtccgatcga cgacccgctc gtaggtctct
      901 ttgtccattg gacctacgcg ccccacaccg tcgacggcgt cgtgggtaag gacagccccc
      961 tcttgctcgc ctccaatttc tcgggcacgt ggcccggact agtggcactg ctcaataccg
     1021 gggcctcgct cgaatgcgtc ggccggccgg cgtcgcgcat ctggaccgat gcacccgagt
     1081 ggaccgccga caacctgttt atggaacgtc tcgaccaatg gtgcacttcc ggcgggattg
     1141 aatatccgat gaccgagctg cgcggggctg gcgccatcag tcccgagtcg aatgccctgg
     1201 cggcacaagt tgaagcatcc attcggcgca agagaatcct ggccctgatg ctcggcgata
     1261 cgtcgatggg catgatcaac ggctacttcg gtcctcgcac gctctatccg atcggctttt
     1321 ccgagcacaa ggtcgatcag gcgtggctca tcgagcggac caaggccatt agccaatcgc
     1381 gcgtcgacga tgccttccgt ttcgtcaagg accgtggagt cacctttcat tggaaggaag
     1441 aagatgcgct cgacttcacc gaggcggcca cccgcgagca gcttcgcggt tacttggccg
     1501 tcctcgacct gatttcggag ttcggagccg actgcgtggg ctggcaatac cagctcggat
     1561 tgctcaacct ccttccgccg agtgactttg ccgaaggact cttgaactcg cacgcgcgac
     1621 ccgagggcaa cggccacgtg atgatcacca gcaccgaggc cgaccaggga aacctggtgc
     1681 ccatggagct gatgaaacga gtcctcgagg ccaagggatt gcccggcgcc gtcatgttcc
     1741 acgatgtccg ctggggagcg cgccatgacg gtcgattcct ctgggtgctc ttgaattcgg
     1801 gaagctgcgg cgcttacgcg ttcaaccaag acgtcgattc gctggcaggc acgcacagct
     1861 tccggcaacc tgcgggttat ttcccggtgc ccgggggcac attcgccggc gtcagcatgc
     1921 ccggtccgat cacctggaca cgctcctgga tcgaccggac cggccagctc gtcatggatc
     1981 tcggccgggg cgaatcggtc gccttgcccg cggacatccg cgaggcctgg tggcgcggaa
     2041 ccacgcgcca atggccgttc atggccgccg atctcggttg ctcgatggaa accatcatgg
     2101 cccactatat gagcaaccac atcgccgttg cctacggcga catcttcggc gagatggccg
     2161 ccctgtcgca atcgctcggt ttcaaagttc gagtcctcgc gagctcttga tgttggtgca
     2221 aatcagcaac tgcacgatgg ccaatcgcgg aatcatgacc gatagttaga ttggagtgtc
     2281 gtccacgccc tgcgcgccaa tgcgtcggcg atcgatttct tcaggccccg acggagcaac
     2341 cgcttctcgc cacagtcaac cagtgctaaa ctcaacgaga ggacgtgacc gaattcgttc
     2401 agaccaagtc cgcgagagaa gactggccat ggaactcgtg gagatttcct ccgttggacg
     2461 cggtagggcc atccgcgtac accagccggg cccgcgatgt tgccgtcttt tccgggtgcg
     2521 atcgggacga ttggtcttgg ccgcaagtct actgtggctc gtctgggtga ccgatcgagc
     2581 gctggggcag tctccaacgg gccagacgca atctcacgcg tcgttctcgg ctcaacagtc
     2641 gctactgaac aagactcgtc aaccatctca tcagacgatc gcgcgaacgg tctcggctcg
     2701 cccgcgggca gaccagcctc cgccactacc gccggcggcc gtcgtctcat ctccggccac
     2761 cgagccgagc gcaccaccgc cgaaacgatt caaggtgcgc gatgaattcg gcaactgcgt
     2821 ggtcgcccga tttcacggcg aatttggcgg caa
//
LOCUS       DATAQL010000274         4402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4607033, whole genome shotgun sequence.
ACCESSION   DATAQL010000274 DATAQL010000000
VERSION     DATAQL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4607033"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1294)
                     /locus_tag="VHS97_04690"
     CDS             complement(149..1294)
                     /locus_tag="VHS97_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX3447524.1"
                     /translation="MSAVAAVASVLLIYLVLLEAFETMILPRRVTRHFRFTRLFYLFS
                     WSPWSAVARLIASGRRRGTFLSLFGPLSILALFGFWAVGLIFGFALLHWSLGTPLNGT
                     GHNIDIYEYFYFSGVTFFTLGFGDVTPSRPLGRFLAITETGIGFGFLAVVISYLPVLY
                     QAFSRREVTISLFDARAGSPPTAGTLLARNSRHGDVSQLIQFLAEWERWSAEVLESHI
                     SYPVLSFYRSQHDNQSWLAALTAVLDASALVVAGAQAAARAQAYMTFAMARHVVVDLA
                     QALQAPPVYPETNRLGPERAENLWRLLREAGLELTNVGAIEQQLAELRGMYEPFVHAL
                     SLRFMMPLPPVFPEGEPVDNWQTSAWMRRTRGFRQLADAVGDDDHED"
     gene            1629..3743
                     /locus_tag="VHS97_04695"
     CDS             1629..3743
                     /locus_tag="VHS97_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX3447525.1"
                     /translation="MTEETIDPGLLEQTKSQIRKLVAEIADLAESDIQPNEFYVEFLN
                     RSVAAVAATGGAFWMMDGRGGLRLQYQLEFGATGLMDGRVKTAPHDALLGCMLQASQA
                     QIVPPSAVIEGVPQAFNPTEYALIISPLMVDKQVVGLLEILMDPTRRAAQQKSTLRFV
                     SDLCDLAATYLKNRQMRQMMSQQRLWNQLEGFTHQIHGSLDLKETAYAVVNDGKRLVA
                     CDRLTVALKIGGRTMVEAVSGQEVVEQRSNLVRELTRLCKVVIRSGEDLVYTGNTDGL
                     APDIRDALEMYVDESGSKVVVATLLHKPEAIDTGPETKVKDKVPFGCLVAEQIGDELA
                     PTDMHARTEVVSRHASTALFNSQEHHKIFLKPLFKALGSPWRMLRGRTLAKIGAALAV
                     VLAVIAAMAFVPCTLTIEGHGSLLPEARQKIYAPVAGIVGEVLVDHDARVKKGDILAK
                     LDSFELQKELKQLFLDNTKALSQMNSLEIQAQKLNSSQENQEQLQVRAQQAEARITAK
                     YTREQIEILNDQIESMNIRSPQDGIITTWEAKKNLLGRPVEIGTELLQVAATDGDWIM
                     EVEVPDDDMGPILAAHSKLEKEIAEGKKKVGTTLPAYFVPMTGPETLYEGYVVRIAPG
                     AETMAESEQYKNRHIVKVTVGFSDAVRKDYLARNQIDEMRPGAEVRARVDCGRTNLAY
                     YLLRKPIQVFYESVLFRWPFLH"
     gene            3836..>4402
                     /locus_tag="VHS97_04700"
     CDS             3836..>4402
                     /locus_tag="VHS97_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447526.1"
                     /translation="MAISKTIAAVAAGCLIMGAAALAQNGGAASRGNNAAVQSVVLEE
                     LAGVDWIERSDVAALHEGVIDSMELQIGMPVKKDGTIGFLHREMAELTVKKAKLQAEA
                     IAPTEKAKAQVFVAASKVARDKLLNERKKGMVSAEEAAEHEGEYRVADAARQEAKENQ
                     AIAKADLDLAERTLKEHTIVAPFDGIIIK"
BASE COUNT          978 a         1290 c         1346 g          788 t
ORIGIN      
        1 acccccgaca agtattacgc cttgcaagac caagtgactc gtatgcgcgg aatcgagagc
       61 ggtcaccggc atgccgaagc ctacatccgg catgtccaga gccctgattt tgcgctccct
      121 ctcgccggga atgcgtgagc agatgcgatc aatcctcgtg gtcgtcgtcc cccacggcgt
      181 ctgccagttg gcgaaatccc cttgtccggc gcatccaggc acttgtttgc cagttgtcca
      241 ccggttcgcc ctcggggaaa accggcggca gcggcatcat gaatctcaat gacagggcgt
      301 ggacgaacgg ctcgtacatc cctctcaact cggcgagctg ctgctcaatg gcccccacat
      361 ttgtcagttc caaccccgct tctcttaaga gtcgccacaa gttctccgcg cgttccgggc
      421 cgagccgatt cgtttcgggg tagaccggtg gggcctgcaa ggcttgggcg aggtcgacga
      481 ccacgtggcg agccatggcg aatgtcatgt acgcctgcgc ccgcgcggcg gcttgtgccc
      541 ccgcgaccac cagcgccgag gcgtcgagca cggccgtaag agcggccagc caagactggt
      601 tatcgtgttg agaacggtaa aaactcagaa ccgggtatga gatatgactt tccaacacct
      661 ccgccgacca ccgctcccac tccgcgagga actggatcag ttgactgacg tcgccgtgcc
      721 ggctgttacg agccaagagt gtgcccgccg tcggcggcga gcccgcccgg gcatcgaaca
      781 gactgatcgt cacctcacgg cgtgaaaacg cttggtacag gaccggcaag tagctgatga
      841 cgaccgcgag gaagccaaag ccgatccccg tctcagtaat ggccagaaac cggcccaatg
      901 gccttgatgg ggtcacgtcg ccgaacccta gtgtaaagaa agtgacgccg ctgaagtaga
      961 aatactcata gatgtcaata ttgtgtcctg tgccattgag cggggtgccg agcgaccagt
     1021 gtagcaaggc gaagccgaag atcagcccga cggcccagaa tccgaagagc gccagaatcg
     1081 acagagggcc aaagagactg aggaatgtcc cgcgccggcg gcctgaggcg atcaatcggg
     1141 ccaccgcgga ccacgggctc cacgaaaaaa ggtaaaagag ccgggtgaat cggaaatggc
     1201 gggtcacccg tctgggcagg atcattgtct cgaacgcttc gagcagcacc aggtagatca
     1261 ataacacgct cgcgaccgcg gcaactgccg acatcaaagg tcctccgtct ctgagagcga
     1321 acaacgttgt ggctggatca tcggccgacg tcggctgaaa ccgcgggcta gtttagccgg
     1381 tgttctcgca tggcgcaagt tgatttgtgg caatttgggg tgtccgggcg gtttcggacg
     1441 ccagttcgca gccgagattg tcccacaaaa aatcctcagg aagattccca agctcaccgt
     1501 cccgttatca gtgcgcagta cgcgccgcgc gctcaagcga cttctgtccc gcgatcgccg
     1561 ttcgaggaag tgcgggctct tcccggcgaa gcaccgcgtg acgcaggcga ttacccactg
     1621 gtacccccat gacggaagaa accattgatc cgggtctgct cgagcagaca aaaagccaga
     1681 ttcgcaagct ggtggccgag atcgccgatc tggcggagtc ggacatacaa cccaacgagt
     1741 tttacgttga gtttttgaac cgatctgtcg cggccgtcgc ggcgaccggc ggcgcgttct
     1801 ggatgatgga cggtcggggc gggctgcggt tgcaatatca gctcgagttt ggcgccaccg
     1861 gtttgatgga cggtcgagtc aagaccgctc cacacgatgc actcttgggt tgcatgctcc
     1921 aggcctcgca agcgcagatc gtgccgccga gcgcggtcat cgagggtgtg ccgcaggctt
     1981 tcaaccccac ggaatatgcg ctgatcatct ctcccttgat ggtggataaa caggtcgtcg
     2041 gcctgctcga gatcctgatg gatcccacgc ggcgggctgc ccagcaaaaa agcacgctca
     2101 ggtttgtcag cgacctttgc gatctggcgg cgacctatct caaaaaccgc cagatgcgac
     2161 agatgatgtc gcagcagcgc ctctggaatc agctcgaggg attcacccac cagatccatg
     2221 gttcgctcga tttgaaagag accgcctatg ccgtggtcaa cgacggcaag cggctggtcg
     2281 cttgcgatcg gctgacggtg gcgctcaaaa tcggcggccg gacgatggtc gaggcggtca
     2341 gcggtcaaga ggtcgtggaa cagcggtcta acctggtacg cgagctaacc cggctttgca
     2401 aggtcgtgat ccgttcgggc gaggatctgg tttataccgg caacaccgat ggtttagcgc
     2461 ctgatattcg cgacgcgctc gagatgtatg tcgatgaatc aggatctaag gttgtcgtcg
     2521 ccacactcct gcacaagccc gaggcgatcg acacgggacc agagaccaag gtcaaggaca
     2581 aggttccctt cggctgcctg gtggccgagc agatcggcga cgagctggcg ccgacggata
     2641 tgcacgcccg caccgaggtc gtgtcgcgac acgcttcgac ggcccttttc aattctcagg
     2701 aacaccacaa gatctttttg aagcccctgt tcaaggccct cggttctccc tggcgaatgc
     2761 taagggggcg gacgctggcc aagatcggcg ctgctttggc agttgtgctc gctgtgatcg
     2821 ccgccatggc attcgtcccc tgcactctca cgatcgaggg gcacggctcg ctcttgccgg
     2881 aggcacgcca gaagatctac gcgccggtgg ccggtatcgt cggcgaggtc ttggtcgatc
     2941 acgacgcgcg ggtgaaaaag ggggacattc tggcaaaact cgacagcttc gagctgcaga
     3001 aagagctgaa gcagttgttt ctcgacaata caaaagcctt gagccagatg aactccctgg
     3061 aaattcaagc tcaaaaactg aactcaagtc aagaaaacca agagcagctc caggtacggg
     3121 cccagcaagc cgaggccagg atcacggcca agtacaccag agaacaaatc gagatcctga
     3181 atgaccagat cgagtcgatg aatattcgct cgccccagga tgggatcatc acgacctggg
     3241 aggccaagaa aaacctgctg ggccggccgg tcgagatcgg caccgagctg ttgcaggtcg
     3301 ccgcgacaga cggcgactgg atcatggagg tcgaggttcc tgatgacgac atgggaccga
     3361 ttctggcggc tcacagcaag ctcgagaaag aaatcgccga agggaagaag aaagtcggca
     3421 cgacactgcc ggcgtacttc gtgcccatga ccggtcccga gaccctctac gaaggttacg
     3481 tcgtgcggat cgccccaggc gccgagacga tggccgagtc cgagcaatac aaaaaccgtc
     3541 atatcgtcaa agtgacggtt ggattcagcg atgctgtccg caaagactac ctggcccgca
     3601 atcagatcga tgagatgcgt ccgggtgccg aggtgcgagc ccgggtcgac tgcggcagga
     3661 ccaacctggc ctattacttg ttaaggaagc caatccaggt cttctacgaa tcggtactat
     3721 tccgctggcc gttcttgcac tgatagatcg ggtcggtccc ggctgattaa ctcggctgat
     3781 gtgaatcgtg tttgatcaac ggcaacaccc tggagtcgag gaggctcgtt caatcatggc
     3841 gatttcaaaa accatcgcgg cggtggccgc aggttgcttg ataatgggcg cggcggcgct
     3901 tgcccagaac ggcggcgccg cgtcgcgtgg caacaacgcc gcggttcagt cggtcgtact
     3961 cgaagagctg gcgggggtcg actggatcga aaggtcggac gtggccgccc tgcacgaggg
     4021 ggtcatcgac agcatggagc tccagatcgg aatgcctgtc aaaaaggatg gaacaatcgg
     4081 cttcttgcac cgcgagatgg ccgagctgac ggtgaaaaaa gccaagctgc aggccgaagc
     4141 cattgccccc acagagaaag ccaaggccca ggtctttgtc gccgcctcca aggtggcgcg
     4201 tgacaagctg ctgaacgaac gtaagaaggg gatggtctct gctgaagaag ccgccgagca
     4261 cgaaggcgaa tatcgcgtcg ccgatgccgc gcggcaagag gccaaagaga accaagcgat
     4321 cgccaaggcc gatctcgacc tggccgaacg gaccttgaaa gagcacacca tcgtcgcccc
     4381 cttcgatggc atcatcatca ag
//
LOCUS       DATAQL010000275         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1096596, whole genome shotgun sequence.
ACCESSION   DATAQL010000275 DATAQL010000000
VERSION     DATAQL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096596"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VHS97_04705"
     CDS             <1..704
                     /locus_tag="VHS97_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3447527.1"
                     /translation="DASHQLRTPITALLGQLEVARRRERTVAEYQQVLDQVHGEARRL
                     GQIVESLLFMARAENEAGRPDLRPLELASWLRAHFSAWSSRERGGDLSLEIELEPPVW
                     VNVHAHLLGQLLDNLVDNACKYSSTGSPIHVRLWRGGTMVALAVEDHGFGLSAEDLPH
                     VFEPFYRSSASRRRGVTGVGLGLAVVERIAWVFGGSITAESEPGQWTRFVLRLSEATE
                     PRSVPDHAQPSLAMS"
     gene            complement(713..1585)
                     /locus_tag="VHS97_04710"
     CDS             complement(713..1585)
                     /locus_tag="VHS97_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEX3447528.1"
                     /translation="MKRLSLNIIAVAHTAILAAFVSGAWASPAGAASPFPDKNLEAAI
                     RGVLKHEPNVELNDEKLQNVYILEASGKDIKDLSGLEKCKNLASIKLNKNKISDLKPL
                     KDLANLQSLDLADNEIKDIGPLANLKALQYIELSNNQVEKLESLSGLTNLTSLWLGSN
                     AIKDIAPLATLVKLWTLSVPKNQIKDINALEKVTKLSVLDLCDNQVEDVSPLSKQTEL
                     SLLLIERNQIKDLKPLVEAAKADSAGAKRFAPYLRLYLAGNPLPDSVKSAQLTDLKAA
                     GVRLEEQKPPGQSP"
     gene            1779..2246
                     /gene="trxA"
                     /locus_tag="VHS97_04715"
     CDS             1779..2246
                     /gene="trxA"
                     /locus_tag="VHS97_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX3447529.1"
                     /translation="MSDTTTSSSFVIDATSENFQQDVIERSHSVPVVMDFWASWCGPC
                     LALGPVLEKLAREYDGRFLLAKVNTDKETELALRFGVRSIPAVFGVRDGKPVDAFVGV
                     QPESVIKSWLDRLMPTPAEALAAEATMLERSDPRAAEEKYSAALSLTPDLPAA"
BASE COUNT          441 a          678 c          747 g          519 t
ORIGIN      
        1 gtgacgctag tcaccagttg cgtaccccca tcacggcgct actcggtcag ctcgaagtcg
       61 cgcgacgccg cgaacgcacg gtcgccgaat atcagcaagt tttggatcag gtccacggcg
      121 aggcgcgcag gcttggccag attgtggaat cgcttctttt catggcgcgc gcggagaacg
      181 aggccggcag gccggactta agaccgctcg aactggcttc ttggctgcga gcgcacttta
      241 gcgcctggtc gagccgcgaa cgcggcggtg atctttccct ggagatcgag ctcgaacctc
      301 ccgtctgggt caacgtccat gcgcacctcc tgggccagtt gctcgacaac ctcgtggata
      361 acgcgtgcaa atacagttcg accgggtcgc ccatccacgt ccgcctctgg cgaggtggca
      421 cgatggtggc gctcgccgtg gaagaccatg gcttcggcct cagtgccgaa gacctgccgc
      481 atgtattcga gccgttctac cgttcgtctg caagccgccg ccggggcgtc acgggtgtcg
      541 gcctgggact ggccgtggtc gaacgcatcg catgggtatt cgggggatcc atcacggcgg
      601 aaagcgagcc cggacagtgg acccgctttg tgctgagact tagtgaggcg accgagcccc
      661 gatccgtacc cgatcacgcc cagccttcat tggcgatgtc gtagcgtgtg attcacgggc
      721 tctggccggg cggtttttgc tcctcgagcc gcacgccagc cgcctttaag tctgtgagct
      781 gtgccgactt gaccgagtcc ggaagggggt tgcccgcaag gtagagccgg agataaggag
      841 caaaccgttt ggcgccagcg gagtcagcct tggctgcctc gacgagcggc tttaaatcct
      901 tgatctgatt ccgctcgatc aaaagcaaac tgagctccgt ctgcttggac aacggactga
      961 cgtcctcgac ctgattgtca cacaagtcaa ggaccgagag cttggtgact ttctcgagag
     1021 cattgatgtc tttgatctga ttcttgggca cggacaaagt ccaaagcttc accagtgtag
     1081 ccaagggggc gatgtctttg atggcgttgc tacccaacca gagggacgtg aggttggtaa
     1141 gaccgctcaa agattcgagc ttttcaacct gattgtttga caattcgatg tattggagag
     1201 ctttgagatt ggcgagcggg ccgatgtctt tgatctcatt gtccgcgaga tcgagcgact
     1261 gcaggttcgc caggtccttg agtggcttca agtcgctgat cttgtttttg ttgagcttga
     1321 tcgaagccag gtttttgcat ttttccagac ccgataagtc tttgatgtcc ttgcccgatg
     1381 cctcgagaat gtatacgttt tgcagttttt catcattcaa ttcgacgttg ggctcgtgct
     1441 tgagtactcc ccggatcgcg gcctcgaggt tcttgtctgg gaaaggcgag gcggcaccgg
     1501 ccgggctggc ccaagcgcct gacacgaacg cggccagtat ggccgtgtga gcgactgcga
     1561 ttatgttcaa ggatagccgt ttcatgggtt atcaggtcct cgggttggaa ctcgacatca
     1621 agtgcctgtt ggcgatgaaa tagtgtggga gagcccccgc ggtctcgcgc cgggcgatac
     1681 attggagcat acgccggcgg ttgcttcggg aaaagaggta ggagcggccg gctgaaacac
     1741 ttgttcatcg ccatccacaa ccccaaaggg accattcaat gagcgacacg acgacgagct
     1801 cctcgtttgt gattgacgcg acgtccgaaa actttcagca ggacgtgatc gagcggtctc
     1861 attccgttcc ggttgtgatg gatttctggg cctcgtggtg cggtccgtgc cttgcgcttg
     1921 gtccggtttt ggaaaagctg gcacgggaat acgacggcag gtttctgctc gccaaggtca
     1981 acacggacaa ggagactgag ctggcacttc gtttcggcgt gcggtcgatt cccgcggtat
     2041 tcggtgttcg cgacggcaag cccgtcgacg cgttcgtcgg cgttcagcct gaatcggtga
     2101 tcaaaagctg gcttgaccgg ctgatgccca cgccggctga ggcgcttgcg gccgaggcca
     2161 caatgcttga gcgatctgac ccgcgggccg ccgaagaaaa gtatagcgcg gccctgtcgc
     2221 tcacccccga tctgcccgcc gcctagatcg gcctcgcgcg gatcgcgctc gagcaggggc
     2281 gcctcgagga cgcccaggcc aggatcttcg gcctcgagcg tcgcgggttt ctcgagcccg
     2341 aagcagagaa aatcaaggcc gagctgacgc tgcgcctcca ggctg
//
LOCUS       DATAQL010000276         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1554392, whole genome shotgun sequence.
ACCESSION   DATAQL010000276 DATAQL010000000
VERSION     DATAQL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554392"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VHS97_04720"
     CDS             <1..1287
                     /locus_tag="VHS97_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX3447530.1"
                     /translation="ADQGETLTFDVLLGADVYIDTGRRLMKRLAEHYHPLASHPFDDV
                     PLVELLTAFELAAEDLTGLCRQIPGGDLIALSHWRRAVQVAGYITKANDVYAFVSPFL
                     NPITGLTRLGTREWIVKPAWKSMQQNVLRWFYQAYVNRLGMHLIELLSGRLAIGARQY
                     RRLTRRPVAGIGADEELPPLTIAVAGARGAGQSRLTALLKEASTGDPHLIKARVESLG
                     LAPSLIDRLRDARWIETPGYPRSDVAETRRDRYQRQSSVAAASESDLLILVVDGCIQD
                     HAPDIAFAHAWDKWFREHPHRELPPTLVVVTGVDRPEFGAGWSAAPAGSTQEAVRESL
                     VRAQFDALRAVLPPTFQDFAAVGLGGETPFGLIEHIVPTLAPLLLRAEHTALLRRLQE
                     LSGKSKVGRLVRQLGEHGRSIWGGLKARHTSQTKSR"
     gene            1697..2068
                     /locus_tag="VHS97_04725"
     CDS             1697..2068
                     /locus_tag="VHS97_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX3447531.1"
                     /translation="MALEHEALTERINGAAIEVHRRLGPGFLESVYEKALLIELKKRG
                     LAVRDQMEVIIGYDGEEVGRHRLDLFVEDTIVVDLKAIKNLADIHFAVVHSQLKAVGR
                     QHGLLLNFAKITLEPKRVIAS"
BASE COUNT          436 a          655 c          664 g          431 t
ORIGIN      
        1 gccgatcaag gtgaaacctt gacgtttgac gtcctcctgg gggccgatgt gtacatcgac
       61 acgggccgcc ggctgatgaa acgattggct gagcactatc atcctctcgc ctcccatccg
      121 ttcgacgacg tgcccttggt cgagctcttg accgcattcg agctcgccgc cgaagacctt
      181 acaggtcttt gccgccagat cccaggtggc gacctgattg cgctctcaca ctggcgacgg
      241 gccgtccagg tggccggcta catcaccaag gccaatgacg tttacgcttt cgtttcgccg
      301 ttcctaaatc cgatcacagg attgacgcga ctgggcactc gcgaatggat cgtgaagcca
      361 gcatggaaat cgatgcagca aaacgtgctg cggtggttct atcaggccta cgtcaaccgt
      421 ctgggaatgc acttgatcga gctattgagc ggccggctcg ccatcggtgc ccgtcagtac
      481 cgccgactga cgcgccggcc cgtggctggc ataggtgccg acgaagagct tccaccgctg
      541 acgatcgccg tggccggcgc tcggggcgcg ggacaatcgc gattgactgc actgctcaag
      601 gaagcctcca cgggtgaccc acatctgatc aaggcccggg tcgaatcgct gggactggcc
      661 cccagcttga tcgaccggct cagggacgct cgatggatcg aaacgcccgg ctaccccagg
      721 tcggacgttg cggagaccag gcgtgatcgt taccagcgac agtcatctgt ggctgccgca
      781 tccgagtcgg acctgctcat tctcgtcgtc gacggctgca tccaggacca cgctccggac
      841 atcgcgttcg cccacgcctg ggacaaatgg ttccgcgagc atccccaccg ggagttgccg
      901 ccgaccctcg tggtggtgac aggcgtggat cgacccgagt tcggcgccgg ctggagcgca
      961 gcaccggccg gctcgacaca agaggccgtg cgcgagtcgc tcgttcgtgc ccagttcgac
     1021 gctctccgcg ccgtccttcc tccaacgttc caggatttcg ccgcggtcgg cctcgggggc
     1081 gaaactcctt ttggcctgat cgagcacatc gtgccgacgc tggcgccgct cttgctccga
     1141 gcggagcaca ctgccctgct tcgacgtctc caagagcttt cgggtaagtc gaaagtcggg
     1201 cgtctcgtcc gccagctcgg cgaacacggc cggtcgatct ggggtggact gaaggctcgt
     1261 cacacttctc agaccaagtc gcgttgacaa cgcggcgcgc gcacaatggc gcccggcgaa
     1321 aaaagagcgt ccagccacat ctctctactt ggtcttctct tacgtgtgac ggcggaataa
     1381 tcggaacagc gacagccgcc gcaactcatc gcaaatgggg atcggaatcg ggagggcgag
     1441 gctccgtccg agccggctcg ccaggaggat cgccctggcg tgatgaaaag ttggccttgc
     1501 agcgccgcga gattcccgcc aattttgttg cgcgaattgt ccctcctgaa gcgaagagcg
     1561 gtttcgaccc attggaaccg cggacggagg gaaaggggac atctcaacgg tcgagatgaa
     1621 tcatcgagcc cctggtggct gagacaccaa ttcatgaagt gagataaaga aaccaggaaa
     1681 gcaggagaga agaaaaatgg ccttggaaca cgaagccttg accgagcgaa tcaatggagc
     1741 agcgatcgag gtgcaccggc ggctcgggcc gggttttctc gaatcagttt acgagaaagc
     1801 cctcttgatc gagttgaaaa aacgcggttt ggcggtgcgg gaccagatgg aggtgatcat
     1861 tggttacgat ggcgaggaag tggggcgtca tcgactcgat ctttttgttg aggataccat
     1921 tgtcgtggat ctcaaagcga tcaagaacct ggctgacatc cactttgcgg tggttcactc
     1981 gcaactgaag gccgtgggta ggcagcacgg tttactgctg aacttcgcca agataacact
     2041 cgaacccaag cgagtcattg cctcttgagg ctgttcctgc tttcctgctt tccttatacg
     2101 gattgagcgg gaatctccgc ccgatagcgc aaccgatttg attcccgaga tcacactcag
     2161 ggtactgtcg tgtgtagtct gcgccg
//
LOCUS       DATAQL010000277         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3996927, whole genome shotgun sequence.
ACCESSION   DATAQL010000277 DATAQL010000000
VERSION     DATAQL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3996927"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VHS97_04730"
     CDS             complement(<1..731)
                     /locus_tag="VHS97_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447532.1"
                     /translation="MAQAQTPLDQSQGDGDGVFIEQVLLALDGLLEAEGVAALQTQII
                     AEASKRRLFVQLCLHAQALAEVVGTQQQDEATEDRSDLRAFEPRSTGGDVRRAFSLRI
                     DSLGRNRSSSRRSTRSWMLVTIAAAACVLALLINGWFFWSRPLRVSQGPGATTIAKPD
                     QPRPVGELQKIADANPVAIMIKLDGAVWEPAGGPGPAERDVLAARRLRLRSGRATLAF
                     LSGVTLTLEGPGDLELISIDRVFCR"
     gene            complement(746..1222)
                     /locus_tag="VHS97_04735"
     CDS             complement(746..1222)
                     /locus_tag="VHS97_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447533.1"
                     /translation="MLLRERVKILAYIRSIVRDNHLAEDVFQEVAVLAVRKCAEIRDG
                     RHFLAWMRLTARHRALKVARREHRYLLMDERLLEHLDDQWAEHDMTSSSDLVEALRHC
                     VERLSPHARNLITLRYVDGISGPRLAEVVDRKLNAVYVALSRIHRSLGDCIKQRLA"
     gene            1500..2474
                     /locus_tag="VHS97_04740"
     CDS             1500..2474
                     /locus_tag="VHS97_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3447534.1"
                     /translation="MENPVNRREFIARGSLLPAGALLGVGLGGGVGCPHASAATPIKR
                     LGGPKLKTSLNAYSFSKTLNDQLKGRGAGTSLFDLLEFCAELDFDAIDPTGYFFPGYP
                     KVPADNYVNDFKRRAFQLGIELSGTGVRNDFASPDKAKRAADVRHVKEWVECAARMGA
                     PVLRVFAGPQPDGYTWDQVAAWMADDLKDCVEHAKKYGVLIGIQNHGDTLKTADDVLK
                     IVKMVDSEWFGVIVDTGYFLSPDPYEDIARVLPFAVNWQIKEKVDGAAGHSKTDLKRL
                     VRIIRAGGYRGYLPIETLSIRGGTENYDPRARVKQLLWELRDALKQPE"
     gene            complement(2509..>3249)
                     /locus_tag="VHS97_04745"
     CDS             complement(2509..>3249)
                     /locus_tag="VHS97_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447535.1"
                     /translation="LAGCDRLIAAAAKAPNLAVHVEFQRRSNPRFQDGVELIGRGELG
                     ALIEARATWTSSNGPMNGHGGWLGRRDRSGDWMVEHAVHVWDILQWFTRESPVRASGW
                     GRRGLFAKTDPLRDVTDHYGVELEWADGFHASFVQSWVAPADDGFTGSSLRVMGEEGG
                     FDFVSGALTFRDRARPRQTIQPGPQADSRMALEAFLASVRSDSTIAPPLSLADARAAT
                     EIGLLVRKAVDLQRVVGLDEIDGWNQRA"
BASE COUNT          625 a         1043 c          997 g          584 t
ORIGIN      
        1 tcccgacaga acacccggtc gatcgagatg agctcgaggt cgcctgggcc ctccaaagtc
       61 agcgtcacgc cgctcaagaa ggcaagcgtg gcacggcccg aacgcaggcg cagccgacgt
      121 gccgccagca cgtcgcgctc cgcggggccg gggccgccgg ccggctccca gacggcgccg
      181 tccagcttga tcatgatcgc cacgggattc gcgtcggcga tcttctgcaa ttcaccgacg
      241 ggacgcggct gatccggctt ggcgattgtc gtcgcccctg ggccctgtga tacgcgcagc
      301 gggcggctcc aaaagaacca cccattgatt aagagagcaa ggacgcaagc agcggccgcg
      361 atggtcacca gcatccagga acgcgtcgat cgccgcgagc tgctccggtt tcgaccgaga
      421 ctgtcgattc tcagagaaaa agcgcggcgt acatcaccac cggtagagcg cggttcgaac
      481 gcccgaagat cggaccgatc ctccgtcgcc tcgtcttgtt gctgcgtacc gaccacctcg
      541 gcgagcgcct gcgcgtgcag gcacaattgc acgaacagac ggcgcttgct ggcctcggcg
      601 attatctgcg tttggagcgc cgcgacccct tccgcctcga gaagcccgtc gagcgcgagc
      661 agaacctgct cgatgaagac gccgtctcca tcgccttggg actgatccag tggcgtctgg
      721 gcctgagcca tgagaacctt tctatttacg ccagacgttg cttgatgcaa tctcccaggg
      781 aacggtgaat gcggctcaag gccacataga ccgcgtttag cttgcgatcg acgacctccg
      841 ccaggcgcgg accgctgatc ccgtccacgt aacgaagcgt gatcaggttg cgcgcgtgcg
      901 gactcagccg ctcgacgcaa tgccgcagtg cctcgaccag atccgatgac gaagtcatgt
      961 cgtgctcggc ccattggtcg tccagatgct cgagcaggcg ttcgtccatg agcagatacc
     1021 gatgctcgcg gcgcgcgact ttcaaggcgc ggtgccgcgc cgtcagtcgc atccaggcca
     1081 ggaaatgacg cccgtcccgg atttccgcac atttgcggac cgccagcacg gcaacctctt
     1141 gaaacacatc ctcggcgagg tgattgtcgc gcacgatcga cctgatatag gccaggatct
     1201 tgacccgctc gcgcaacaac aaaacaacga gagattcttg atcgatggga tgggcccccc
     1261 atttgctagc gccctattct gcatatcccc cgacacctcc ggaactgtac agcaaatccg
     1321 gcatttttcg gttttgcgct cgatggttcg aaatcgagga aaaatggaca cgtcggccct
     1381 ggagaaacat acgggttctc tgtaccattc ccatcgctcg ccctgttgcg ccgcgttggc
     1441 tgcgggcggg cgcgactcgc atcagattgg aacccatttt gcatccggga ggtccgacgg
     1501 tggaaaatcc agtcaatcga cgggagttca ttgcccgcgg ttcgcttttg ccagccgggg
     1561 cgttgttggg cgtcgggctc gggggcgggg tcggctgccc gcacgccagc gccgcgacgc
     1621 cgatcaagcg gcttggtggc cccaagctca agaccagtct gaacgcctac tccttcagca
     1681 agacattgaa cgaccagctc aagggacggg gagcaggaac ctctctgttc gaccttctcg
     1741 aattctgcgc ggagctggac ttcgatgcca ttgatcccac cgggtatttc tttccagggt
     1801 acccgaaggt tcctgctgat aattatgtga atgatttcaa acgaagggcg ttccagctcg
     1861 ggatcgagct cagcgggacg ggcgtccgca acgacttcgc ctcgcccgac aaagcgaagc
     1921 gtgccgccga cgtccgccat gtcaaggagt gggtcgagtg cgctgcccga atgggggcgc
     1981 cggtgctgcg cgtcttcgcc ggaccccaac ccgacggcta cacctgggac caggtggctg
     2041 cctggatggc cgacgacctg aaagactgcg tcgagcatgc caagaaatat ggggtcttga
     2101 tcggaattca gaaccacggc gacactctga aaactgccga tgacgtcctc aagatcgtca
     2161 agatggtcga ttccgagtgg ttcggcgtga ttgtcgacac cggctacttc ctgtcgccgg
     2221 atccttacga ggacatcgcg cgcgtgcttc cctttgccgt caactggcag atcaaagaaa
     2281 aggtcgacgg cgccgccggg cattcgaaga ccgacttgaa gcggctcgtg cggatcatcc
     2341 gcgcgggagg ctatcggggc tatcttccga tcgagaccct gtcgatcaga ggtgggaccg
     2401 aaaattatga tccgcgtgcc cgggtgaagc aattgctctg ggagctgcgc gacgcgctca
     2461 agcagccgga gtgagggccg gtgtcacgcg aggagccgcg aagccgggct atgcccgttg
     2521 attccaccca tcgatttcat ccagcccgac cacgcgctgt aggtcgacgg ccttgcggac
     2581 gagcagaccg atttccgtgg cggcacgggc atcggccagg gataaggggg gtgcgatcgt
     2641 tgaatccgag cggaccgatg cgagaaacgc ctcgagcgcc atccttgaat cggcctgagg
     2701 tcccggctgg atcgtctgac ggggacgagc acggtcccgg aacgtgagcg ctccactgac
     2761 gaaatcaaag cccccctctt cacccattac gcgcaggctc gacccggtga agccgtcgtc
     2821 ggctggcgcg acccagctct gcacgaagga ggcatgaaac ccgtcggccc attccagttc
     2881 gacaccgtag tgatctgtca catcacgcaa cgggtctgtc ttggcgaaaa gcccgcgccg
     2941 accccaaccg ctggcccgaa ccggcgactc gcgcgtgaac cattgcagga tgtcccagac
     3001 gtgcacggcg tgttcgacca tccagtcgcc cgaacgatcg cgtcggccca gccatccgcc
     3061 gtggccgttc atggggccgt tgctgctggt ccaggtggca cgggcctcga tcaacgcccc
     3121 tagctcgcct cggccgatca gctcgacccc gtcttgaaac ctggggttcg accgcctttg
     3181 aaattcaaca tggaccgcca ggttgggcgc tttggcggcc gcggcgatca gtcgatcgca
     3241 gccagccag
//
LOCUS       DATAQL010000278         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_944052, whole genome shotgun sequence.
ACCESSION   DATAQL010000278 DATAQL010000000
VERSION     DATAQL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944052"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /gene="ppdK"
                     /locus_tag="VHS97_04750"
     CDS             <1..1787
                     /gene="ppdK"
                     /locus_tag="VHS97_04750"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX3447536.1"
                     /translation="LKEEMPKVYDQLLQIRKKLETHYKEMQDIEFTVQSGTLYMLQTR
                     TGKRTGSAAVRIAVEMAKEKLIDETTAIKRVSPDSLNHLLLPQLDPKAKRNAFARGIA
                     ASPGAACGKVVLSAIAAIEHHEKHPEDPILLVRKETSPEDVAGMHLAKGILTATGGKA
                     SHAAVVARGWGKPCVVGCDAIHIDEKGGKFSIGAHTVKAGDFITIDGTTGDVMIGKVP
                     TVDPSISGDFATFMSWADKVRTLKIRTNADTPEDSARARSFGAEGIGLCRTEHMFFGD
                     QRIVAMRKMILADDEAGRKAALDELEPFQKQDFIGIFEAMEGLPVTIRLLDPPLHEFL
                     PRDDNPSGQAEVARQLGISVEKVKRRVEQLHEANPMLGLRGCRLPIKFPEIGDMQVRA
                     IIQAAIEVKKRGKSVLPEIMIPLVGILEELTILKKRAIAIAEEEFKKSGVTVEYQIGT
                     MIEIPRAALAADQIALEAEFFSFGTNDLTQLTFGFSRDDIGSFMPAYVEQKILPVDPF
                     QTIDVNGVGQLIEIGVSKGRKSRKEKHGEHLKVGICGEHGGDPESVVFCHKAGLDYVS
                     CSPFRVPIARLAAAQAALAGGGAISRDK"
BASE COUNT          385 a          544 c          551 g          311 t
ORIGIN      
        1 ggctcaagga agagatgccc aaggtctacg accaattgct gcagattcgg aaaaagctcg
       61 aaacgcatta caaagagatg caggatatcg agttcacggt acagtccggc acgctctata
      121 tgcttcagac gcgcaccggc aagcgaaccg gcagcgcggc cgtgcggatc gccgtcgaga
      181 tggccaagga gaagttgatc gacgaaacga ccgcgatcaa gcgggtcagc cccgacagcc
      241 tcaaccacct cctcttgccc cagctcgatc ccaaggccaa gcgaaatgcg tttgcgcgcg
      301 ggattgcggc cagtcccggg gcggcctgcg gcaaggtcgt cctctccgcc attgcagcca
      361 tcgagcatca tgagaagcat cccgaagatc ccatcctgct ggttcgaaaa gagacgagtc
      421 ccgaggacgt ggccggcatg catctggcca agggcatcct gacggccacc ggcggcaaag
      481 ccagccacgc ggctgtcgtc gcgcgagggt ggggaaagcc ttgcgtcgtc ggatgtgacg
      541 ccatccatat cgacgagaag gggggaaagt tctcgatcgg agcgcacacc gtcaaggccg
      601 gcgatttcat caccattgac ggcacgacgg gcgatgtgat gatcggcaag gtgccgactg
      661 tcgacccctc gatctcgggt gacttcgcga cattcatgtc atgggccgat aaggtccgaa
      721 cactcaagat ccggaccaac gccgatacac ccgaagactc ggcccgcgcg cgctcgtttg
      781 gtgccgaggg gatcggcctg tgccggaccg agcacatgtt tttcggcgac cagcgcatcg
      841 tcgccatgcg caagatgatc ctggccgacg acgaggctgg gcgaaaggcc gctctcgacg
      901 agctcgagcc atttcagaaa caggacttca tcggtatctt cgaggcaatg gaagggctgc
      961 cggtcaccat ccgcctgctc gatccgccgt tgcatgagtt tctaccccgt gacgacaacc
     1021 cgagcggtca ggcagaggtt gctcgccagc tcgggatctc cgtcgaaaag gtcaagcgcc
     1081 gcgtcgaaca gcttcacgaa gcgaatccga tgctcggttt gcgtgggtgc cggttgccga
     1141 tcaagttccc ggagattggc gatatgcagg tccgggcgat catccaggct gccattgaag
     1201 tcaagaaacg tggcaagtcc gtcctgcccg aaatcatgat tccgctggtc ggaatccttg
     1261 aagagctgac catcctcaaa aagcgggcga tcgccatcgc tgaggaagag ttcaagaagt
     1321 caggcgtcac ggtagagtat caaatcggca cgatgatcga gatacctcgc gccgccctgg
     1381 ccgctgacca gatcgccctg gaggctgagt tcttctcgtt cggcacgaac gaccttacgc
     1441 agttgacatt cggcttctcc cgcgacgaca tcggctcgtt catgccagct tatgtcgaac
     1501 agaaaatcct cccggtcgac ccgttccaga cgatcgacgt caatggcgtc ggtcagttga
     1561 tcgagattgg cgtctccaag ggccgcaagt cccgcaaaga aaagcatggc gaacacctga
     1621 aagtgggaat ctgcggtgag cacggcggcg accccgagag cgtggtcttc tgccacaagg
     1681 cggggctgga ctacgtctcc tgctcgccgt tccgcgtgcc gatcgcccgc ctggccgccg
     1741 cgcaggccgc cctggccggc gggggggcca tcagcaggga taagtaaggt g
//
LOCUS       DATAQL010000279         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1097035, whole genome shotgun sequence.
ACCESSION   DATAQL010000279 DATAQL010000000
VERSION     DATAQL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097035"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1015
                     /locus_tag="VHS97_04755"
     CDS             311..1015
                     /locus_tag="VHS97_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3447537.1"
                     /translation="MNIGVGVIRGHLITLRRFLLTFWRDFRAGQGLTRRGGGTLLVDL
                     FRGPGRGSEPTIRQDFNTTGLFTVEYPDERLPVYERFRVLPVLIYDTEDGNVRCTSCN
                     ICAKVCPPQCIWMSQAKSPKGTVVPLPEDFYIDMDVCMNCGLCSEYCPFDAIKMDHNF
                     ELSNYERHQTHIYNLQDLLVSSEFYAKTHPEAWNSPEEIAERNKVTKKKDQRLQKALA
                     ATKPAAAKPAPKAVTA"
     gene            1097..1369
                     /locus_tag="VHS97_04760"
     CDS             1097..1369
                     /locus_tag="VHS97_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447538.1"
                     /translation="MIPAPPVPQYPLGTRLRVTQRVRVGHRRWVTQVEGVVVHESRRP
                     VGGIEMGGKASYCHQKTLRLKRDDGELTEIAVDQATQVETIAKPAS"
BASE COUNT          327 a          440 c          448 g          294 t
ORIGIN      
        1 cacgtgcgca gcccgagttt catcaacctg accgcgcttg aggcaatgtg tctgggccac
       61 accattgccg atgtcgtggg catcctgggt agcctcgaca tcgtgctggg cgaggtagac
      121 cggtgatggg ttggcactcc gcggatggcc tgcaaagaag aggcatcatg cggggagccg
      181 gcgcatgcga tcgttcgcca tcatcagatc gcggaaggta acggctgagt cttcaaaatc
      241 aacagcttga tttgataagt cgagaatcgt cgggcgggcc gcgggccgcg ttggacggaa
      301 aggacaagcg atgaatattg gcgtgggggt catcaggggg cacctgatca cgctgcggcg
      361 gttcttactc acgttctggc gcgatttccg cgccggccag ggcctgacca ggcgaggagg
      421 aggcacgctc ctggtcgatc tgtttcgtgg cccggggcgc ggtagcgagc cgacgatccg
      481 ccaagacttc aatacgacag gcctcttcac ggttgagtat cccgacgagc gactgccggt
      541 ctacgaacgg ttccgcgtgc tgccggtcct catttacgat accgaggacg gcaatgtacg
      601 gtgcacgtcg tgcaatattt gcgccaaggt atgccccccg cagtgcatct ggatgagcca
      661 ggcaaagagc cccaagggta cggtcgtccc ccttcccgag gacttttaca tcgatatgga
      721 tgtctgcatg aactgcggcc tttgctcgga gtattgcccg ttcgacgcga tcaagatgga
      781 ccacaacttc gagctgtcca actacgagcg gcatcagacc catatctata accttcaaga
      841 cctcttggtc tcgagcgagt tttacgccaa gactcatccc gaggcctgga acagccccga
      901 ggaaatcgcc gagcgaaaca aggtgaccaa aaagaaagac cagcgcctcc aaaaggcact
      961 ggccgccacc aagccggccg ccgcgaaacc agcccccaaa gccgtgacag cttaaacttt
     1021 gcagggtatt gcccttgcat cttatcaacc tataagatgc ttgctcccca agaacactgc
     1081 tcgaggatcg gccaaaatga ttcctgctcc ccctgtaccc caatatccgc tgggaacccg
     1141 gttgcgcgtg actcagcgcg ttcgcgtcgg tcatcgccgc tgggtcactc aggtcgaagg
     1201 ggtggtcgta cacgagagcc gccgtccggt gggcggcatc gagatggggg gaaaggcgtc
     1261 gtactgtcat cagaaaaccc tgcgactgaa gcgcgacgac ggtgagttga ccgagatcgc
     1321 cgtcgatcaa gcaacgcagg tcgagaccat cgccaagccg gcttcgtgaa atggagcgag
     1381 actggccgcc aacgttctta tggctatgcc gatccctggc ggcccagctt aacgccctgg
     1441 caggaacaga gagttcaaat ggtaatttac aattacatct ttgtaggtct gctgcttctc
     1501 ctgggtatc
//
LOCUS       DATAQL010000280         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_944214, whole genome shotgun sequence.
ACCESSION   DATAQL010000280 DATAQL010000000
VERSION     DATAQL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944214"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="glgP"
                     /locus_tag="VHS97_04765"
     CDS             <1..1141
                     /gene="glgP"
                     /locus_tag="VHS97_04765"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEX3447539.1"
                     /translation="SELLKTEVLRDFNTLWPEKFVNVTNGVTPRRWVALSNPDLTSLI
                     SRRIGNEWIRDLEKLRDLERHVDDPEFRADWMRVQRATKVRLAAYIREKIGVLVEPAS
                     MFDVQVKRIHEYKRQHLNVLHIAALYLHLKRNPELVIPPRTFLFGGKAAPGYYMAKLI
                     IKLITSVGETVDNDPDVRDRLKVVFLPDYNVTLGQRVYPAADLSEQISLAGKEASGTG
                     NMKFAMNGAVTIGTLDGANVEIREEVGPENFFLFGLTTPEVMARKAAGYSPSEYYHSN
                     GTLREVIDALSNGVFSHGDRSIFQPLIRSLLDRDEYMLFADFQAYLDCQARVSEAYLD
                     VDRWSRMSILNSARSGKFSSDRSIRDYSRLIWHVDGFKVDTAGEP"
     gene            complement(1138..>2167)
                     /locus_tag="VHS97_04770"
     CDS             complement(1138..>2167)
                     /locus_tag="VHS97_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX3447540.1"
                     /translation="SLMTMSGAVVMGMVYAFLTARVLSARLEFRRRPARIPRSRHIVV
                     IGMGPLGRRVAGLLADLNQPVVAVSDVELDHSVLPRIPVIRGEPRENLAKANCATAAS
                     VMALTDDDVSNLELALMSAQINENCRLVVRTDDAEFGRSVTSLAPHTLAMSVYALSAE
                     AFAAAALGENVLNLLRIGHETILTTEFTVEPGDTLEGHLLGDVTCGYGLAAIVFQRGR
                     SDPVQFFPPEDIRLEVGNRLVVLATIGGLQNAEYGVTPQRTHQIRVLTVYSERAAFEG
                     ARAISRITGCNLGTAGSLFEQLPAVLPQPLFRHQARRLAHELGVIGVIAEVVPAQSAA
                     GAGTPSCD"
BASE COUNT          426 a          657 c          671 g          413 t
ORIGIN      
        1 cagcgaactg ttgaaaaccg aggtactccg cgatttcaat actctctggc ctgagaaatt
       61 cgtgaatgtg accaacggcg tgaccccccg gcgatgggtt gcgctcagca atccggacct
      121 cacctcgctt atctcgcgtc gcatcgggaa tgagtggatc cgcgacctgg aaaaactgag
      181 ggatctcgaa cgacacgtcg acgatcccga gttccgcgcc gactggatga gagtccagcg
      241 cgccacgaag gtccggctgg cggcttacat tcgcgagaag attggcgtcc tggtcgagcc
      301 ggcgtcgatg tttgacgttc aggtgaagcg cattcacgag tacaagcgcc agcatctcaa
      361 tgtcctccac atcgctgctc tctatttgca cctgaagcgg aatcccgagc tggtcattcc
      421 gccgcgcact ttcctgttcg ggggcaaggc ggcgccgggc tactacatgg ccaagctcat
      481 catcaagctc atcacctcag tgggagaaac cgtcgacaac gatcccgatg tgcgggaccg
      541 gttgaaggtg gtcttcctgc ccgattacaa cgtaacgctt gggcagcgag tgtatccggc
      601 ggcggatctt tcggagcaga tttcgctggc cggcaaagag gcttcgggta cgggcaacat
      661 gaagttcgcc atgaatggcg cggtgacgat cgggacgctc gatggcgcca acgtcgagat
      721 tcgcgaggaa gtggggccgg agaacttctt cctgttcgga ttgacgacgc cggaagtgat
      781 ggcccggaaa gcggcgggat attctccgtc ggagtattac cactccaatg gcacgctgcg
      841 ggaggtcatc gacgcattgt cgaacggcgt cttctcgcat ggtgaccgct ccatctttca
      901 accactgatc cgttctctgc tcgatcgcga cgaatacatg ctgttcgccg atttccaggc
      961 gtacctggac tgccaggcgc gcgtgagcga ggcgtatctt gacgtggacc gctggagccg
     1021 gatgtcgata ctcaactcag cgaggtcggg caagttctcg tcggaccgct cgatacgcga
     1081 ttacagccgg ctgatctggc atgtggacgg cttcaaagtg gataccgcgg gcgagcctta
     1141 atcgcaggat ggtgtgcctg cgccggcggc cgactgcgcc ggaacgactt cggcgatgac
     1201 gccgatcacg cctagttcat gcgccaaccg tcgcgcctgg tgccggaaca gcggttgggg
     1261 gaggaccgcc ggcaattgct cgaacagcga gcccgccgtc ccgagattgc agcccgtaat
     1321 ccgggaaata gcgcgcgcgc cttcaaatgc cgcgcgctcc gagtacacgg tcagcacccg
     1381 tatctggtga gtcctctgcg gggtcactcc gtactccgca ttttgcagtc cgccaattgt
     1441 ggccagaacc accaggcgat ttccaacttc caaacggata tcctcgggcg gaaagaactg
     1501 caccggatcg gaacgcccgc gctggaaaac gatcgcggcc agtccgtagc cgcaggtcac
     1561 atcaccgagc agatggccct ccagcgtatc gccgggttcc acggtgaatt ccgtggtgag
     1621 aatcgtctcg tgtccaatac gaagcaggtt gagaacgttc tcgccaagcg cggcggccgc
     1681 gaaggcctcg gcggacagtg cgtagacgct catggcaagc gtgtgcggag ccagtgatgt
     1741 cacgctgcgg ccgaattcgg cgtcgtccgt ccggaccaca aggcggcagt tctcgttgat
     1801 ttgtgccgac atcagcgcca attcgaggtt ggagacatcg tcatcggtca gcgccatcac
     1861 gctggcggcc gtggcacagt tggcttttgc caggttctcc cgcggctcgc ccctgatcac
     1921 aggaattcgc ggaagcacgc tgtgatcgag ttcgacgtcg ctcacggcga ctaccggttg
     1981 gttgaggtcg gccagcagtc ctgcgacgcg gcgcccgagc gggcccatgc cgatcaccac
     2041 gatatgcctg gatcgcggaa tgcgggcggg acgacgccgg aattccaggc gcgccgatag
     2101 cactcgcgcc gtcagaaatg catacaccat acccatgacc accgcacctg acatggtcat
     2161 caggctg
//
LOCUS       DATAQL010000281         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_487129, whole genome shotgun sequence.
ACCESSION   DATAQL010000281 DATAQL010000000
VERSION     DATAQL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487129"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VHS97_04775"
     CDS             <1..205
                     /locus_tag="VHS97_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447541.1"
                     /translation="KAKAICGKTNRGSYRDEDACRRFSRYENLRHEKPSRGLLSPVPA
                     REAGRSARCRSIVAFGSRDGKVC"
     gene            complement(544..>1538)
                     /locus_tag="VHS97_04780"
     CDS             complement(544..>1538)
                     /locus_tag="VHS97_04780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX3447542.1"
                     /translation="YRWDDGPLDSPFWSKTLAAAVRLRSEILDLGKNSSAYRLIFSEG
                     DGLSGLTVDRYDRWLVAQFTSLALYTHRDLMLGLLTELTDAEGIIARTDRGVTPKEGL
                     RAGQESTVGKVPVEPVTIVENGLHFRVDIQSGQKSGFYLDQRVNRREVASYCHGKRVL
                     DLFCFSGAFSLNAARNGAVETLGVDSSAPAIELARQNAALNGIENIEFIVGDVLEVLQ
                     RLELAQRRFDVVICDPPKFARQAKDVEHALKGYLRLNMAALEVLKPDGILVTCSCSGL
                     IGRELFTDLLGNVAEQSRRPIQILEQRGQAPDHPVSASCLETEYLKCVICRVEG"
BASE COUNT          323 a          458 c          447 g          310 t
ORIGIN      
        1 caaggccaag gccatttgcg gtaaaaccaa ccgcggcagc taccgcgacg aggacgcctg
       61 tcgacgcttt tcgcgttacg aaaacttgag gcatgagaaa cccagccgcg gactgttgtc
      121 gcctgttcca gcccgtgagg ccggccgatc ggcgcgatgt cgctcgatcg tagcctttgg
      181 gtcgcgagac ggcaaggttt gctagaaacc gctcggcatc ggacggccgc gcggccgatc
      241 agcgtcgcat acggcgtaaa tttttatatt gactccttcc cacgctcggc gcttgtcatt
      301 cccgagaatt tgctatcatg gttggctgaa ttctaccagg acgctcatgg ctggagccaa
      361 ctatgttcac atggaagtca attcaccagg ccccgccagc ggaatgggct cgcgcaccgc
      421 gggagcgtgg gaacaagcca tcggtcgccc cgagcgaaat gcttaacggc gttggcggag
      481 gggtcagttt agtgttgcga ggggggcaat tttagtgtcg accgacaacg gcatcgcgct
      541 tcctcatccc tcaacgcggc agatcacaca cttcaagtat tcagtttcga ggcagcttgc
      601 cgagaccgga tggtcgggcg cctggccacg ttgttcgaga atctggatcg gacgtcgcga
      661 ttgctcggcc acattgccca gcagatcagt aaacaactcg cggccgatca atccggagca
      721 cgagcaagtg accaggatac catcgggctt gagcacttcc agtgcagcca tgttcaaccg
      781 caggtagccc ttgagcgcat gttcgacgtc cttggcctgt cgcgcaaatt tcggcgggtc
      841 gcagatgacc acgtcgaacc gccgttgcgc gagttccagg cgctgcaata cctcaagcac
      901 gtcgccgacg ataaattcga tattctcgat cccattcaag gccgcgttct gtcgagccag
      961 ctcgatcgca ggtgccgagc tgtccacacc cagggtttcg accgcaccat ttctggcggc
     1021 gttgagtgaa aaagctccgg aaaagcaaaa tagatcgagc acccgcttgc cgtgacagta
     1081 cgaggcgacc tcgcggcgat tgacgcgttg atccagataa aagcctgact tttgcccgga
     1141 ttgaatatcg actcggaaat ggagaccatt ctcgacgatc gtcaccggct cgacagggac
     1201 ttttccgacc gtcgattcct ggcccgcgcg cagtccctcc ttgggtgtga cgccacggtc
     1261 ggtccgggcg ataatccctt cggcgtctgt caattcggtg agcagcccga gcatcagatc
     1321 acggtgcgtg tagagcgcca ggctggtgaa ttgcgccacc agccagcgat cataacgatc
     1381 gacagtaagt cccgagagac cgtccccttc gctgaagatc agacggtatg ccgacgagtt
     1441 tttgcctaag tcgagaattt cgctccgcag ccgcactgct gcggccagcg tcttggacca
     1501 aaatgggctg tcgaggggac cgtcgtccca acgataga
//
LOCUS       DATAQL010000282         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4456285, whole genome shotgun sequence.
ACCESSION   DATAQL010000282 DATAQL010000000
VERSION     DATAQL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4456285"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VHS97_04785"
     CDS             <1..204
                     /locus_tag="VHS97_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3447543.1"
                     /translation="DSNTGKMIFTLAEQIAQLSSRITLYPGDVILTGTPAGVGAGRGE
                     FLKTGDMVKLWIEKIGTLTNTMA"
     gene            complement(263..781)
                     /locus_tag="VHS97_04790"
     CDS             complement(263..781)
                     /locus_tag="VHS97_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3447544.1"
                     /translation="MAMTEDKTTPAKTDKPPPALKLKFLSHGTLESRDLAFSRKFYEE
                     FLGLDVVQTSPISLLVRLGGDHTYAVVYSKQPREMDLLNHNGLDVSTKEEVDAAHRAV
                     VDSAQKWKLTKISKPHLQHGTYSFYFWDADGNSWEILTNPEGGYSWLFEKGDLEGKGH
                     LDRSFKRPGVDI"
     gene            871..1209
                     /locus_tag="VHS97_04795"
     CDS             871..1209
                     /locus_tag="VHS97_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447545.1"
                     /translation="MRVEIAVKRAAVNPIWYNDTAIIVIEISGMVGQTAESVAVKTEG
                     AMAGSAERMRRCRARRRAGFRCFTVELHRSEIDTLVRRGLLRADERADEGAVVDALAQ
                     YIVQTLAPPL"
BASE COUNT          321 a          447 c          507 g          340 t
ORIGIN      
        1 gattcgaaca ccggcaagat gatcttcacg ctggccgagc agatcgcgca actgtcgagc
       61 cgcatcacgc tctatcctgg cgatgtgatt ctcaccggaa cccccgcggg ggtcggcgcc
      121 gggcgcggcg aattcctcaa gaccggtgac atggtcaagc tgtggatcga gaaaatcggc
      181 acgctgacca acacgatggc gtgacccagg cggctgggcc agccaacggc tcgggaggcc
      241 gccgtgtttt agaaggtgtg gcctagatgt cgacgcccgg tcgcttgaag ctccgatcga
      301 gatggccctt gccctcaaga tcaccctttt cgaagagcca gctataacca ccctcgggat
      361 tggtgaggat ctcccaagaa ttgccgtccg cgtcccagaa gtagaagctg taggtcccgt
      421 gctggagatg gggctttgag attttggtga gtttccactt ctgggcggag tcgacgacgg
      481 cgcgatgcgc cgcatccact tcctccttgg tgctgacatc gagaccgttg tggttgagga
      541 ggtccatctc gcggggttgc ttgctgtaga cgaccgcgta ggtgtgatcg ccgccaagcc
      601 ggacgagcag cgaaatgggg ctcgtctgca ccacgtcaag gccgaggaat tcctcataaa
      661 acttgcgcga gaaggcgagg tcgcggcttt cgagtgtgcc gtgcgatagg aacttgagct
      721 tgagagccgg gggcggctta tcggttttag ccggcgtggt cttgtcttcg gtcattgcca
      781 tgggggcgtc tccactcggt ctgacattcc cggatttcga cggcaacgtg tcattttggg
      841 ccattcgtgg caacatgctt cggtcgcgga atgcgggtcg agattgccgt aaagcgtgcg
      901 gcagtcaacc caatctggta caatgacact gcgataatcg tcattgagat cagtggtatg
      961 gttgggcaaa cggcagaaag tgtggccgtc aagaccgaag gcgccatggc gggttcagct
     1021 gagcgaatgc gtcgttgccg ggcgcgacgt cgggccggct ttcgctgttt tacggtcgaa
     1081 cttcaccgca gcgaaataga cacactcgtt cgccgcgggc tcctgcgggc agatgaacgt
     1141 gccgacgaag gcgccgtggt cgatgcactt gcgcaataca tcgtgcaaac gctcgcgccg
     1201 ccattgtgac cgacaacgcc gagttgccgt gacgcataat ggggacagcg caacagcgat
     1261 tcgaattccc gattttgaca ttcagcgtcg tccaaaaccg tgcacgtgta tattgtactt
     1321 cagccaacgg aatatctgct cggggtgcac ctcgatgacc gtttggcaca tgtatgggcg
     1381 ctccccggcc tcgacatcga gggaaccctg aaatacggtt cgtcgccggt ctgagaggat
     1441 cttaaagtga ggcagttcac cgggatgttg ctggcattga tgtgcatggc aacatgctct
     1501 ggttgcaaaa cgtcgcccga attggtgcta accatcacgc cctcgtcggt cacgatcccg
     1561 gacgaatcca agcgaggaag gcctttagcc actgtctcgg ctcgctggag cgacg
//
LOCUS       DATAQL010000283         8766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5219092, whole genome shotgun sequence.
ACCESSION   DATAQL010000283 DATAQL010000000
VERSION     DATAQL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5219092"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VHS97_04800"
     CDS             <1..973
                     /locus_tag="VHS97_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447546.1"
                     /translation="ITGMAGLLQLPGVIVALMRVYAPIALLLLTGVAVYLLPNLGSYR
                     LAEAVSIAANEGLNQRWPGPGSSLQVMTGFFAMAGLLSVDWGARLERRRDLLWGGLAS
                     VVLVASVTSVLSLVVVAGAVTRLVSDGATPTASAINASPLSFRWAVVHGIGGVPGGAI
                     LILFGLASLAPACFSVWVYGQRLSTHWPRLRQSGWTWSGGLIAFVLGATSCVNRLDLV
                     YSAMGDIFAPVVGVIVGNWVRLRGKWSGFRRGINPSAVIAWGAGALVGMILDVFRMFN
                     REYSDWCQPTSVCGFLTSAVAFGLLTRMESPAPSLAPTADDPVHSNE"
     gene            1011..2312
                     /locus_tag="VHS97_04805"
     CDS             1011..2312
                     /locus_tag="VHS97_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447547.1"
                     /translation="MAESATGGSVFRSVRSRTFAALDHPDFRRYYMGQGISLVGTWLQ
                     GAAVRWLVFDQTHSEFMLGVIEVASLMPGLLVGLFAGALADRVVPLRMVVLMEVGQMV
                     LALLLAVLVWLQTFQVWHLALILALARICVTFELPSRQVFFFDLVGPEILSNAIALNS
                     GLFNATRVVGPALAGVCLSLVGAAGCFALNGASYLAAIAAILSIRLPERPRPLGREDF
                     TIKEITGGLSYLRTDRRIFAQFALVAFFGFVGMGYEAMVPAYARRVVDTGVYGYSVLL
                     ATSGIGATVGAFVVASLGGLLRKERLTIAGMVLFGACLAGAALLPSYFPPASDGLSRL
                     VAAAVCLLGAGFGAVLFYSSSMTLIQLAAPDHLRGRVMGIWMIVYSGSVPLGALWTGR
                     VAQSWGVSFVMGFSAVLCMVVGLVVWASGVLAPMRHVRAPE"
     gene            complement(2461..3762)
                     /locus_tag="VHS97_04810"
     CDS             complement(2461..3762)
                     /locus_tag="VHS97_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3447548.1"
                     /translation="MSRDLEGDKPPKPRRPRPAIDPGLVADRESPGDEYATYQPSPVG
                     STAGGKSRGSKKQGGVNPLAEKFEAPVGGPTWWERILFGSVSTGQLAQFCRQFGSYLQ
                     AGVDINKSLSSLERQFSGTALGPILGRIQISIRRGSTLEAAMAREPQAFGTMFLSMIK
                     VAETRGGVPETLKMLARHYEARQRLLRQARSAMIYPVIVITVACVVVALITIFLLPIF
                     AATLKEIAGNRPLPLPSQVLLGISGFVKYIGWWLIPLLMAGTPILLFNLYKTPPGKAI
                     MDRLVLATPVFGTLCRKLDTSRFARTLSVLLDAGVDVGSSIDLTADVLRMTPIRQAVR
                     STREQIIEGKELSGVLDRTRQFSPDVIAVVETGEETGKLPETLAHLADDYDEQISTMV
                     ANLGHLVQPIMIVCLGAMVLFIILAVLMPIIQMISSLAAPG"
     gene            complement(3909..5366)
                     /gene="gcvPB"
                     /locus_tag="VHS97_04815"
     CDS             complement(3909..5366)
                     /gene="gcvPB"
                     /locus_tag="VHS97_04815"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX3447549.1"
                     /translation="MYKLDQPPLVFESSRPGRYTAILPAADVPDKPLSSLIPDSHMAR
                     SRPPLPELGELDVVRHYTNLSTLNMSIDSNFYPLGSCTMKYNPKRNERLAGLPGLSAQ
                     HPYQDESTLQGLLAILFDLQQILAEIAGLEAVSLQPAAGAQGELTALLVAAAFFRDRG
                     ERRTQVLIPDSAHGTNPASAHLAGFEAITIKSDSRGLVDLADFKAKLSDETAVFMMTN
                     PNTVGLFDPQIGEIATLIHDRGALLYLDGANMNAILGIVRPGDMGVDLMHYNPHKTFS
                     GPHGGGGPGAGPIAVRGDLAPYLPAPVVARNADGVFRLDHDRPRSIGRVRAFFGNTGV
                     LFRAYCYIRSQGPDGLTRIAQNAVLNANYLMSLVNDVYPVPFGNRCMHEFVASARTLA
                     KDRGIRAMDIGKRLIDYNFHAPTVYFPLIVSEALMIEPTESESRETLEAFAKVLRVIA
                     GENPELLHDAPHTTPISRPDEVKAAKTPVLRWKPR"
     gene            complement(5482..6828)
                     /gene="gcvPA"
                     /locus_tag="VHS97_04820"
     CDS             complement(5482..6828)
                     /gene="gcvPA"
                     /locus_tag="VHS97_04820"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX3447550.1"
                     /translation="MAYIANTPDDTREMLAAIGLDRIDQLFDMIPPELRLKTPLQVPP
                     ALTELELTDHITAILALNEGADKRVCFLGGGSYDHFVPAVVDNLASRGEFYTAYTPYQ
                     PEASQGTLQATFEYQTLMTELTGLDVSNASLYDGGSATAEAMLMALAVTRRTGEVVVA
                     GSVHPEYRQILATFLDHLGPKLITVPATDGRVSPESVARHITDQTAAVVVQYPNFLGQ
                     LEEVRDIVEAAHAKGAMAILSVDPISLGLLQRPRDYGADIVVAEGQSLGNPLSYGGPY
                     LGIMACREDYLRKLPGRIVGETTDRNGKRCWVLTLQTREQHIRREKATSNICTNQGLL
                     ALRSCIYLAVMGPTGLRQAAELSTRKAHYAAERLASVPGLSLAFPGPFFKEFVVRSRR
                     DPKQVLADVGRLGYHGGIALERWYPELADCILIAVTEKRTRAEIDGLAAAYAKALG"
     gene            complement(6950..7336)
                     /gene="gcvH"
                     /locus_tag="VHS97_04825"
     CDS             complement(6950..7336)
                     /gene="gcvH"
                     /locus_tag="VHS97_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX3447551.1"
                     /translation="MDLNTLRFAPTHEWVLLEGDVATVGISKFAVDQLTDLIMIELPA
                     VGTNVTAGKSFGEIESVKAVSDLYAPLGGEVIEVNSGVAGNVQLLAEDPFDKGWLVKI
                     KVVPPVDTSGLMDLGTYEKKVAEEGH"
     gene            complement(7410..8531)
                     /gene="gcvT"
                     /locus_tag="VHS97_04830"
     CDS             complement(7410..8531)
                     /gene="gcvT"
                     /locus_tag="VHS97_04830"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX3447552.1"
                     /translation="MSPPANLLETPLHSWHKANGARLVEFGGWSMPVQYTTIVDEHKA
                     VRERVGLFDISHMGRLTFDGPGVLDWLERVTTNHVAHLNDLQIQYSLLATNQGGVIDD
                     ILVYRQPYAYIVVCNATNRDPVKTRFDELRREAEGNFVDRTLDTAMIAVQGPKALETL
                     QPLFGEPLEAVPYYHFTMGQLKGGVNTVISRTGYTGEDGFEVVVASKAAADVWNALLE
                     SGKAHGIEACGLGARDTLRLEAAMPLYGHELSESINPFAAGVGWAVKLDKGDFAGSTA
                     LKDWKDAPGLARVGLKLTSKRIARQGAIVFAEDRPIGEVTSGTFSPTLQTSLAMALVD
                     PSAAAIGTELAIDVRGHREPAQVVKLPFYKRNRKSAHAG"
BASE COUNT         1581 a         2599 c         2716 g         1870 t
ORIGIN      
        1 tatcacgggc atggccggcc tgttacagct ccccggagtg atcgtggctt tgatgcgcgt
       61 ctacgccccg attgccttgc tactcttgac tggggttgcg gtgtatctgt tgcccaatct
      121 gggttcctat cgccttgccg aagcggtctc gatcgcggcc aacgagggtc tcaaccagcg
      181 atggccgggc cccggatcga gcctgcaggt catgaccggc ttcttcgcga tggcgggtct
      241 cttgagcgtt gactggggtg ccaggttaga gcgccggcgc gacctcttgt ggggcgggct
      301 agcgagcgtc gtgctggtgg cttccgtgac atccgtcctt tcgctggtcg tcgtcgcggg
      361 agctgtcacc cggttggtga gtgacggtgc gacgccgacg gccagcgcga tcaatgcgag
      421 tccgttgtcc tttcgatggg ctgtggtcca cggaattggc ggagttcccg gaggcgcgat
      481 tctgattttg ttcggcttgg cgtcgctcgc gcctgcgtgt tttagcgtct gggtctatgg
      541 gcagaggctg tcgacacact ggcctcgtct ccgccagtca ggctggacat ggtcaggggg
      601 cttaatcgcg tttgtcctgg gggcaacttc gtgcgtgaat cgactcgacc tcgtctattc
      661 ggcgatgggt gacatcttcg caccggttgt cggcgtgatc gtggggaatt gggtgcggct
      721 gcggggcaag tggtcgggat ttcgtcgcgg aatcaacccg agcgccgtca tcgcctgggg
      781 agcaggcgct ttggtcggca tgatcctgga tgtctttcgc atgttcaatc gggaatattc
      841 cgactggtgc cagccgacgt cagtttgtgg cttcctcacg tcggctgtcg catttggcct
      901 tctaacaagg atggagtcgc cagccccctc tttggctccg accgcagatg atccggtcca
      961 ttcgaatgag tgaccccacc agacgtgcaa gccgtttccg agggagcttg atggccgaat
     1021 ccgcaaccgg tgggagcgtg ttccgttcgg tccgctcgcg gaccttcgcc gccctggatc
     1081 atcctgactt tcggcgctac tacatggggc aggggatcag tctggtcggg acctggctcc
     1141 agggcgcggc ggtgcgctgg cttgttttcg atcagactca ttcggaattc atgctcgggg
     1201 tcatcgaggt tgcctcgctg atgccgggac ttttggtcgg gctctttgca ggtgctctgg
     1261 ccgaccgcgt tgttcccctg aggatggtcg tactgatgga ggtcggccag atggttctgg
     1321 ccttgctgct agccgtcttg gtttggctgc aaacctttca ggtctggcat ctggcattga
     1381 tcctggcatt ggcgcgcatt tgcgtgacct ttgagctccc gagccgccag gtgttcttct
     1441 ttgacctggt cggaccagag atcctgtcca atgcgattgc gctcaattcg ggcttgttca
     1501 acgccactcg tgtggtcggg ccggcacttg cgggcgtttg cctgtcgctc gtgggggcgg
     1561 ccggctgttt cgccttgaac ggcgcgagct acctggccgc gatcgcggcg atcctctcga
     1621 ttcgattgcc ggagcgtccg cggccactag gtcgcgaaga cttcaccatc aaagagatca
     1681 cgggtgggct ctcctacctg aggacggatc ggcggatttt tgcccagttc gcgctggtgg
     1741 cgttcttcgg ctttgtcggc atgggctacg aggccatggt gccggcctac gcgcggaggg
     1801 tggtcgacac aggcgtctac ggctacagcg tgctgctagc gacgagcggg atcggtgcga
     1861 ccgtcggcgc attcgtggtt gcctcgctgg gcgggctctt gcgcaaggag cggctgacga
     1921 tcgcgggcat ggtcctattc ggagcctgcc ttgccggggc ggcgcttttg ccgtcgtact
     1981 ttccaccggc gtcggacggc ttgtcgcgcc tggtagccgc ggctgtatgc ctcctcggag
     2041 caggctttgg agcggtgctc ttctattcct catccatgac cctgatccaa ttagccgccc
     2101 ccgatcactt gaggggccgg gttatgggaa tctggatgat cgtttactca ggctcggtcc
     2161 cgttaggcgc actctggacc gggagggtgg cgcagtcgtg gggtgtgtcg tttgtgatgg
     2221 ggttctcggc cgttctctgc atggtcgtcg ggttagtagt ctgggcatcg ggtgtactcg
     2281 cgccgatgcg ccacgtccgc gctccggaat agccggagca cgattcttcg aagattgcac
     2341 gctccggcgg cctgctatct tcgaagaatc gtgcaatctt cgcggcctcg tacgcggcac
     2401 atctgggaca acccacgaaa tcgatacttg atcggcttca ctcgtcaccc atgagccggt
     2461 ctatcccggt gcggccaaac tgctgatcat ttggatgatc ggcatcaaaa cggccaggat
     2521 gatgaacaga accatcgcgc ccaagcaaac gatcatgatc ggctggacca gatgccctag
     2581 attggcgacc atcgtcgaaa tctgctcgtc gtagtcgtcg gcaaggtggg cgagggtctc
     2641 gggaagcttg ccggtctctt cgccggtctc gacgaccgca atcacgtcgg gcgaaaactg
     2701 gcgcgtgcgg tcgaggacgc cgctgagctc ctttccctca atgatctgtt cacgcgttga
     2761 tcggaccgcc tggcggatgg gggtcatccg cagcacgtcg gctgtgaggt cgatcgagct
     2821 gccgacgtcc acgccggcat caagcaagac cgagagagtc cgggcaaatc gcgaggtgtc
     2881 gagtttgcgg cagagcgtac caaataccgg agtggccagc accaagcgat ccatgatcgc
     2941 cttgccaggc ggagtcttgt agaggttgaa gagcagaatc ggcgtaccgg ccatcaagag
     3001 agggatcagc caccagccga tgtacttcac aaatccgcta attcccagca ggacctggct
     3061 aggcagcggc aacggccgat tgccggcgat ttctttgagc gtggcggcga atatggggag
     3121 caagaagatc gtgatcaatg cgaccacaac gcacgccaca gtgatgacga tgaccgggta
     3181 gatcatcgcc gaacgcgcct gacgcaacaa ccgctggcgg gcttcgtagt gccgggcaag
     3241 cattttcaga gtttcgggta cgcccccgcg cgtttccgcc accttgatca tgctgaggaa
     3301 catcgtgccg aaagcctgcg gttcccgcgc catcgccgcc tcgagagtcg agccacggcg
     3361 gatgctaatc tgaatacgac ccaggatcgg ccccagcgcg gtcccactga actggcgctc
     3421 gaggcttgaa agcgacttat tgatgtctac tcccgcctgg agatacgagc caaattgccg
     3481 gcaaaactgc gcgagttgac ccgtgctgac gctgccgaac agaatccgtt cccaccacgt
     3541 cggaccgccg acaggcgcct caaacttttc ggcgagcggg ttaactccgc cttgcttttt
     3601 ggatccgcgc gatttcccgc ccgcggtcga accgacggga ctgggttgat aggttgcgta
     3661 ttcgtcgccc ggactctcgc gatccgcaac cagccccgga tcgatcgcgg gtctggggcg
     3721 tctcggcttg ggcggtttgt caccttccag gtctcgactc atcgcgtgat tcctgtcgac
     3781 aatcgaccaa aaacgaccag cgcgaaatgc gttcgattca ctaagcttct tagtctaccg
     3841 ctatcgtctc gcctccagcc ccgccggtca agccgataga atcgacattt gtctgagcaa
     3901 gcgtcgcgtc accttggttt ccacctgaga acgggagtct tggcagcctt gacttcgtcg
     3961 ggacggctga tcggagtcgt atggggcgca tcgtgcagaa gctccggatt ttccccggca
     4021 atgacgcgta gcactttcgc aaacgcctcc agtgtttcgc ggctctccga ctccgtcggc
     4081 tcgatcatca atgcctccga aacgatcaac gggaaataga ccgtcggcgc gtgaaagttg
     4141 taatcgatca gccgtttgcc aatatccatc gcccggatgc cgcggtcctt ggcgagcgtg
     4201 cgggccgacg cgacgaactc gtgcatgcac cggtttccaa acggcacagg atagacgtcg
     4261 ttcacgagcg acatcagata gttcgcgttc aggacggcgt tctgggcgat gcgcgtcaat
     4321 ccgtcggggc cctggctgcg gatgtagcaa taggctcgga acaagacgcc cgtattccca
     4381 aagaacgcgc gcacgcggcc gatcgagcgg ggacgatcgt ggtccagccg gaagacgcca
     4441 tccgcattac gagcgacgac cggcgcggga aggtatggag ccaggtcacc gcggacggcg
     4501 atcggaccgg caccaggtcc gccgccgccg tgagggcccg aaaacgtctt gtgggggttg
     4561 taatgcatca gatcgacccc catgtctccc ggccgcacga tgccgaggat ggcattcatg
     4621 ttggcgccgt cgagatagag cagggcgccg cgatcatgaa tcaatgtcgc gatctcaccg
     4681 atctgagggt cgaaaagccc cacagtattg ggattcgtca tcatgaacac ggccgtctcg
     4741 tcactcagct tggctttgaa gtcggccagg tcgaccaatc cccggctgtc gctcttgatc
     4801 gttatggctt caaagccggc cagatgggcc gaagcggggt tggtgccgtg cgcactatct
     4861 ggaatcaaga cctgggtgcg acgctcgcca cgatcgcgga agaatgccgc ggcgaccagg
     4921 agcgccgtca gctctccctg ggctccggct gccggctgca ggctcaccgc ctctagcccg
     4981 gcgatctccg ccaagatctg ctggagatcg aacaagatcg ccagcaagcc ctgaagagtg
     5041 ctctcgtctt gataggggtg ctgcgccgat aaccctggca gccccgctag ccgctcgttc
     5101 cgcttggggt tgtacttcat cgtgcaggag ccgagcggat agaaattgga gtcgatcgac
     5161 atgttgagcg tcgacaggtt cgtatagtgc cgcacgacat caagctcgcc cagctcgggc
     5221 aggggggggc gcgatcgagc catatgcgaa tcggggatga gcgacgacaa cggcttatcg
     5281 ggaacatctg ctgcagggag gatggccgtg tatcgtcctg gccggctgga ctcgaacacg
     5341 agcggaggtt gatcaagttt gtacatgagc tttggtattt gttggtggtt agttgcaatt
     5401 cttgggaaag aatccgcggc ttccaggcga gagagaatgc cacgtgagtc agccggccgc
     5461 tgcgccttta cgttttgcct tttagcccaa cgctttcgcg tacgcggcgg cgagtccgtc
     5521 gatttcggcc ctggtgcgct tttccgtgac ggcgatcaaa atgcagtcgg cgagctctgg
     5581 gtaccagcgc tctagcgcga tcccgccgtg atatcccagc cggccgacgt cggcgagcac
     5641 ctgcttcggg tcgcgtcggc tgcggaccac gaactccttg aaaaacgggc cgggaaacgc
     5701 cagcgaaagg ccaggtaccg aggccagcct ttcggcggcg taatgggcct tacgagtcga
     5761 gagctcggcc gcttggcgca gaccggtcgg acccatcaca gccaggtaaa tgcaggatct
     5821 gagagccagc aagccctggt tggtgcagat gttgcttgtc gccttctcgc gcctgatgtg
     5881 ctgctcgcgc gtctggagcg tgagcaccca gcatcgctta ccgttacgat cggtcgtttc
     5941 accaacgatt cgcccaggca gcttccggag gtagtcctcg cggcaggcca tgattcccag
     6001 atagggccct ccgtaggata gcggatttcc cagcgactgc ccttcggcga cgacgatgtc
     6061 ggccccataa tcccgtggcc gctggagcag acccaaactg atcggatcga cgctcaagat
     6121 cgccattgct cccttagcgt gggccgcctc gacaatatct cgtacctctt cgagctgtcc
     6181 caggaagttg gggtactgca ccacgaccgc cgcggtctgg tcggttatgt gccgagcaac
     6241 cgactcggga gagacccgtc cgtcggtcgc cggaaccgtg atgagcttgg gaccgaggtg
     6301 gtcgagaaac gtcgccagga tctggcgata ttcgggatgg acggatccag caaccaccac
     6361 ctctcctgtc cgccgagtga ccgcaagcgc catcaacatc gcctcggccg tggcggatcc
     6421 gccgtcgtag agactggcgt tggacacgtc aaggccggtc aactcggtca tgagcgtctg
     6481 atactcgaag gtcgcctgca acgtcccctg acttgcttcg ggctggtagg gggtgtaagc
     6541 cgtgtaaaac tcgccgcgcg aggcgagatt gtccacaacc gccggcacaa aatggtcgta
     6601 gctccccccg cccaggaaac agacccgctt gtctgcccct tcgttcagtg ccagtatggc
     6661 cgtgatgtga tccgtcagct ctagctccgt aagagcggga gggacctgca gcggagtttt
     6721 caagcgcagc tccggcggaa tcatgtcgaa aagctggtcg attctgtcga gcccgattgc
     6781 agcaagcatt tcgcgggtat cgtcaggcgt gttggcgatg taggccatct tgcgtcagat
     6841 ccaattctat aacacaggaa tcgatgttaa tctgaatgat cgaagtgcgc agtgtcagac
     6901 ggttcgggca gaagaggctc ggccgtccca ccgtgttact ttgggccggt caatggcctt
     6961 cctcggcgac ctttttctcg taggtgccca agtccatcaa accggacgta tccactggtg
     7021 gaaccacctt gatcttcacg agccagccct tgtcgaaagg atcttcggcg aggagctgga
     7081 cattgcccgc gacgccgctg ttgacctcga ttacttcgcc tcctaaggga gcgtacaaat
     7141 cgctcaccgc tttgacgctc tcgatctcac cgaagctctt gcccgccgtt acgttggttc
     7201 ccacggcggg tagctcgatc atgatcaagt cggtcaattg atcaacggcg aacttgctga
     7261 ttccaacagt cgcgacatca ccctcgagca aaacccattc gtgcgtagga gcgaatcgaa
     7321 gtgtgttaag atccatgatt caagttcctc atcggatttg ccggttcagg atgagttctt
     7381 agtttcaaat gagcactcga cagcggtttt tagccggcgt gggcactttt gcggttgcgt
     7441 ttatagaagg gcagcttgac gacctgggct ggctcacggt gtccgcggac gtcgatggcc
     7501 aactcggtgc cgatcgccgc cgcggaggga tcgacgagcg ccatcgccag actggtttgc
     7561 agggttggtg aaaacgttcc cgaggtcact tcgccaatcg gccggtcttc cgcaaaaacg
     7621 atcgcccctt ggcgggcgat ccgctttgaa gtcagcttca agccaacgcg cgcgagcccc
     7681 ggcgcgtcct tccagtcctt aagcgcggtg ctgccggcga aatctccttt gtcgagcttg
     7741 acggcccagc ccactccggc cgcgaacgga ttgatcgact ccgaaagctc gtggccgtaa
     7801 aggggcatcg ccgcttccag acgcaacgta tcgcgtgccc ccaatccgca cgcctctata
     7861 ccatgagcct ttcctgattc aagcagggca ttccacacgt cggccgcggc ctttgaggca
     7921 accaccacct cgaatccatc ttcgcccgtg tagcccgtac ggctaatcac cgtattcacg
     7981 ccgcccttca gttggcccat ggtgaaatga tagtacggta cggcttccag cggctcgcca
     8041 aacagaggtt gcaaagtctc caaggccttt gggccctgca ccgcgatcat cgccgtatca
     8101 agggtacggt ccacgaaatt gccttcggcc tcgcgccgta gttcgtcgaa ccgggttttg
     8161 accggatcgc ggttggttgc attgcaaaca acgatatagg cgtacggctg acgatagaca
     8221 aggatgtcgt cgatcacgcc cccttggttc gtggccagga gactgtactg aatctgcaaa
     8281 tcgttaaggt gagcgacgtg gttggtcgtg acgcgttcga gccaatcgag aacaccaggc
     8341 ccgtcgaatg tgagccggcc catatggctg atgtcgaata atcccacccg ctcgcgcacc
     8401 gccttgtgct cgtcgacaat ggtggtgtac tggacgggca tcgaccagcc gccgaactcg
     8461 accaagcgcg caccgtttgc cttgtgccat gaatgcagcg gtgtttctag caagtttgcg
     8521 gggggcgaca cgagggcctc tgggttttgg ttcgatttcc tgcctatcga gctggccgga
     8581 cggagccgta tgattttagc ccgaccgcca ttcagtcgac aacatgagtc agtccgggca
     8641 gcgatccgcc cccgagaaca gcatgctgcc accgagccat gactgacttg agtgaacctg
     8701 gatactcggt tagcgtaaac tcttttgaca tctgcaaggg ataggcacaa cgtggtagta
     8761 gacgat
//
LOCUS       DATAQL010000284         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2012969, whole genome shotgun sequence.
ACCESSION   DATAQL010000284 DATAQL010000000
VERSION     DATAQL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012969"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VHS97_04835"
     CDS             <1..1455
                     /locus_tag="VHS97_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX3447553.1"
                     /translation="WRQAEAGGWLVCAESDLRGDELGASHTLIQDADVLDTWFSSALW
                     PHSTLGWPDETAELERYYPTSVLSTARDIITLWVARMVIFGLFNRGEVPFRDVYIHPV
                     IQDGQGKRMSKSAGNGVDPVDIIDIHGADALRYTLAAGATETQDLRIPVESLKLPDGR
                     LVNTSERFELGRNFANKFWNAARLALMNLDGYEPASLARDDLPIEDRWIIDGLDRTIG
                     QVTSALEQFQFAEAARQLRDFTWGHFCDWYLEFVKGRLRDPAARPLAQRVLAALLDGL
                     SRLLHPIMPFVTEQVWQSLNSLAPVRGLSEPTIAAESVCIAAWPRQIGWSDEAAQQTV
                     DYWQQAITAIRNMKAERNVPKDARITPIIVAHGAVAASLKQGEPFLRSLLPAESITIV
                     SSIERPADCAVTVLPEAEIILPLEGLIDKEAERRKMQKSLADLERQMGGLRAKLANES
                     FTARAPAEVVKETRAKLAELEAQSEAVAGVLNRA"
     gene            1603..>1904
                     /locus_tag="VHS97_04840"
     CDS             1603..>1904
                     /locus_tag="VHS97_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX3447554.1"
                     /translation="MAVQMELTRIIINETSDSHIIFLKEVDGERMFPIVIGIFEASSI
                     DRRVRNQATPRPLTHDLLANAIDLLGGDLQDIYINELREHTYFAKLRIRHEGELVEV"
BASE COUNT          362 a          567 c          607 g          368 t
ORIGIN      
        1 tggcggcagg cagaggcggg tggatggctc gtctgcgccg agtccgattt gcgcggcgac
       61 gagctggggg cgagccatac cctgatccaa gacgcggacg ttctcgatac gtggttcagc
      121 tcggccttgt ggcctcactc gaccttgggc tggccggacg agaccgctga gctcgagagg
      181 tactatccca cgagcgtctt gtccacggcc cgcgacatca tcacgctctg ggtggcccga
      241 atggtcattt tcggtctgtt taaccgcggt gaggtaccgt tccgcgacgt ctacattcac
      301 cctgtaatcc aggatggtca ggggaagcga atgtcgaagt cggcgggcaa tggcgtcgac
      361 ccggtggaca tcatcgacat ccacggcgcc gacgccctgc gctataccct ggcggccggc
      421 gccaccgaaa cccaggacct gaggatcccc gtcgaatccc tcaagctccc cgacgggcgc
      481 ctggtcaata cctccgagcg gttcgagctg ggtcgcaatt tcgccaacaa gttttggaac
      541 gctgcccggc tcgcgctcat gaatctggat ggctacgagc cggcgtcatt agcgcgcgac
      601 gacttgccga tcgaggatcg ctggatcatc gatgggctcg atcgaacgat tggccaggta
      661 acatcggccc tcgaacaatt ccagtttgcc gaggcggccc gccagttgcg cgatttcaca
      721 tgggggcatt tctgcgattg gtacctcgaa tttgtcaaag gccggttgcg cgatccggcg
      781 gctcgtccac tcgcccagcg cgtcttggct gcgctccttg atgggctcag ccggctgctc
      841 catccgatca tgccgttcgt gaccgaacag gtgtggcaga gcctgaacag cctggcgccg
      901 gtgcgagggc tatccgaacc gacgatcgcc gcggagagcg tctgcatcgc ggcctggcct
      961 cgtcagattg gttggtcgga tgaagccgca cagcaaacgg tcgattactg gcaacaggcg
     1021 ataacggcga ttcgcaatat gaaggccgag cgtaatgtcc ccaaggacgc gcggatcact
     1081 ccgatcatcg ttgcccacgg ggctgtcgcc gcgtctctca agcaagggga gccgtttctg
     1141 cggagccttc tgccggccga gtcgatcacg atcgtttcgt cgattgaacg cccagccgac
     1201 tgcgccgtga cggtcttgcc cgaggccgag atcattctgc ctctcgaggg cttgatcgac
     1261 aaagaagcgg aacggcgcaa gatgcaaaag agcttggccg acctcgagcg gcagatgggt
     1321 ggcttacggg ccaagctggc gaacgagtcg ttcacggcgc gggcgccggc tgaggtcgtt
     1381 aaagagacgc gcgccaagct ggccgagctg gaggcgcaga gcgaggcggt cgccggtgtt
     1441 ttgaatcgag cctgactgag gtcgcgcctt gcgttgacct tgcaacggaa ctcctctatg
     1501 atcggaacct ggcgcccgag tcgtcagtcc gaagggtgag acggtgaatc ctgtgtcaac
     1561 ctcacgcttc ctccgctccc cgattgaaga ggtgagagtg ccgtggcggt acagatggag
     1621 ctcacgcgga tcatcatcaa cgaaacgagt gactcgcaca tcatcttttt gaaggaggtt
     1681 gatggcgagc ggatgtttcc gatcgtgatc ggcattttcg aggcctcgag catcgatcgg
     1741 cgagtcagga atcaggcaac cccacgtccc ctgacgcacg acctcttggc caacgccatc
     1801 gatctgttgg ggggggactt acaggatatc tatatcaacg agctgcgtga gcacacctat
     1861 ttcgccaagc tgcgaatccg gcacgaaggg gaactcgtcg aggt
//
LOCUS       DATAQL010000285         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4760619, whole genome shotgun sequence.
ACCESSION   DATAQL010000285 DATAQL010000000
VERSION     DATAQL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4760619"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="serS"
                     /locus_tag="VHS97_04845"
     CDS             complement(<1..1202)
                     /gene="serS"
                     /locus_tag="VHS97_04845"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX3447555.1"
                     /translation="MLDLKFVVANAEAVKQNCRNRNVPADVLEDIDRVVALESERKTL
                     LQAVEEIRRRQNEIAQATGKERDPQKRSALIDEGKRLKLSVAEEEDRLRRLEAEMNQR
                     LRRIPNLTHPDAPVGLTEDASLELRKVGTPRVFDFKPKDHVELGKTLDLIDFETAGKV
                     SGNGFYFLKNDLVLLDLALQQFALKKLIAAGFIPIVTPDLARNNILEGIGFTPRGVET
                     QVYSVEDTELSLIGTAEITLGGMLADEILEESGLPVKYVGLSHCFRTEAGAAGRASRG
                     LYRVHQFTKVEMFAFSTPESSGPIHAQMLAIEEDLFQSLGIPYRVLDICSGDLGGPAY
                     RKFDLEAWMPGRGEHGEYGEVTSTSDCTDYQARRLNIRYRPSGQKGTRFVHTLNGTAI
                     ALSRALIVV"
     gene            complement(1325..>2108)
                     /locus_tag="VHS97_04850"
     CDS             complement(1325..>2108)
                     /locus_tag="VHS97_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3447556.1"
                     /translation="YFELLVQGANRDLHSGTYGGAVANPLNVLATILASLKGPDGKVQ
                     IAGFYDRVKPLEDWERAEFAKLAFSESEFQADLRVSSLEGEAGYTTLERKWARPTCDI
                     NGIFGGYAGPGPKTVLPCKGGAKLSFRLVPDQDPQTVARQFKDHVAKVCPPGVTYEII
                     SHHGAPAVLVDIDTPGVRAAVHAIQASFGNKPVFIREGGSIPVVGLIKQHLGVDTLLL
                     GWGQNDDNLHGPNEKFSLADFHRGIKSSAHLLDELAKESISS"
BASE COUNT          406 a          604 c          660 g          438 t
ORIGIN      
        1 acgacgatca acgcgcggct aagggcgatg gccgttccgt tgagcgtgtg gacgaagcgt
       61 gttccttttt ggcctgaagg acgatagcgg atattgagcc tgcgggcctg gtagtcggtg
      121 cagtccgaag tgctggtgac ctcaccatat tcgccgtgtt cgccgcggcc gggcatccat
      181 gcttcgagat cgaacttgcg ataagccggc ccacccaggt ctcccgagca aatgtcgagc
      241 acgcggtacg ggatgcccag cgattggaac aagtcttctt cgatcgccag catctgggcg
      301 tggattgggc cggatgattc gggcgtgctg aacgcgaaca tctcgacctt ggtgaactgg
      361 tgaacgcgat aaagcccacg gcttgcccgc cccgcggcgc cggcctcggt tcggaagcaa
      421 tgcgacaacc ccacgtactt caccggcaag ccggactctt cgaggatttc gtcggcaagc
      481 atcccgccca gcgtgatctc ggcggtgccg atcaagctga gctctgtatc ctcgaccgaa
      541 tagacctgcg tctcgacccc gcgcggcgtg aacccaatcc cctccagaat attgttacgg
      601 gccaggtcgg gagtgacgat gggaataaat ccggctgcga tcaatttttt gagagcaaac
      661 tgctggagtg ccaggtcgag gagaaccagg tcgttcttga ggaaataaaa gccgttgccg
      721 ctgaccttgc cggcggtctc gaaatcgatc aggtcgagag ttttgcccag ctcgacgtgg
      781 tctttcggct tgaagtcaaa cacgcgggga gtgccgacct tccgcagttc gaggctggcg
      841 tcttcggtca ggccgaccgg tgcgtccggg tgcgtcaagt tggggatgcg cctcaagcgc
      901 tggttcatct cggcctcgag acggcggagg cggtcttcct cctcggccac cgacaacttt
      961 agtcgtttgc cttcatcgat cagtgccgac cgcttttggg gatcgcgctc cttgccggtg
     1021 gcctgggcaa tctcgttttg gcggcggcga atttcctcga cggcctgaag tagggtctta
     1081 cgctccgatt cgagcgcgac aacgcgatcg atgtcttcga ggacgtcggc cgggacgttg
     1141 cgattgcggc agttttgctt gactgcctcg gcattggcga cgacaaactt cagatcgagc
     1201 atgggcacac cttacgacgt aaagctgctt gaataaatta tgatcgctcg cccggccttc
     1261 gggcatgccc gcgagcgttc attaacacga gtggtcgggg tgagcgatct ttttacgcgc
     1321 ggacctaaga agaaatcgat tccttggcga gttcgtcgag cagatgcgcg ctggacttga
     1381 tgccgcgatg aaaatcggcg agtgaaaact tctcgttggg gccgtgcaga ttatcgtcat
     1441 tctggcccca gcccaggagc agggtgtcga cgcccaggtg ctgcttgatc agtcccacga
     1501 ccggaatcga gcctccctcg cgaatgaaga ctggtttgtt gccgaagcta gcctgtatcg
     1561 catggacggc ggcccttacc ccgggggtat caatatcgac aaggacggcc ggagcgccgt
     1621 ggtggctgat aatctcatat gtcacaccgg gcgggcagac cttggcgacg tgatccttga
     1681 attgccgtgc gaccgtttgg ggatcctggt cgggaacgag gcggaagctg agcttggcgc
     1741 cccccttgca ggggagaacg gtcttcggtc cggggccagc ataaccgccg aaaatgccat
     1801 taatgtcaca cgtcggccgg gcccacttgc gctcgagcgt ggtataaccg gcctcaccct
     1861 cgagcgaact cacacgaaga tcggcctgaa attcggattc ggaaaacgcc agtttggcaa
     1921 actcagcgcg ctcccaatcc tcgagcggct tgacgcggtc gtaaaatccg gcgatctgga
     1981 ctttgccgtc agggcccttc aagctggcga ggatcgtggc caaaacgttc agggggttgg
     2041 cgacggcacc gccgtaggtt cccgagtgta agtcgcggtt agctccctga acgagcagct
     2101 cgaaatag
//
LOCUS       DATAQL010000286         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4302391, whole genome shotgun sequence.
ACCESSION   DATAQL010000286 DATAQL010000000
VERSION     DATAQL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4302391"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VHS97_04855"
     CDS             complement(<1..413)
                     /locus_tag="VHS97_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447557.1"
                     /translation="MSAQEATASQQFRLTLTMGQMMKLVVFAAVASACLAVGVEDAAI
                     SSRASWSVWSIVMTEGVAIPLVLAVTAFPLVRQGPQKDLLIRALLTVAIAMAWTDALV
                     YLIRAICLLTKGSHFYKFNWIVTLATVIVLPVPLA"
     gene            complement(431..>1595)
                     /locus_tag="VHS97_04860"
     CDS             complement(431..>1595)
                     /locus_tag="VHS97_04860"
                     /inference="COORDINATES: protein motif:HMM:NF042565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1583 domain-containing protein"
                     /protein_id="HEX3447558.1"
                     /translation="EKSGEGAARPFGADPDRVPWARVTQTRADTRGAGEPISHWGWSA
                     DMLTHFPGHELDLMYLTVPLRGDFQVDCELTGSPDRKIRLQYGGLAIGPNPDLKHLER
                     AQFGRPVAELSVNPPLEKPAEWFPYRLVVKGGRMTAFINGRKIHEAPAPPDVDPWVAF
                     LAQDIQSGAARKFTITGDPRIPDKVNLSALPDLGGWLAAEYGDSVIGDDPDWDKRGEE
                     ITGRVLEDITGSKQESVLRYHRPMIEDGRIEYEFYHDPGKVMVHPALGRLAFLLEPDG
                     VKVHLLTDGAYERSGLTADNARDEPECRRGPTSLPLKPSAWNQLVLSVTGDKVTLELN
                     GQPIYERPIEPLNQRAFGLFHYADQTQVRVRNVSYQGNWPRSLPASLKGVDSH"
BASE COUNT          314 a          492 c          491 g          298 t
ORIGIN      
        1 cacgccagcg gaacgggcaa gacgatcacc gtggccagtg tcacgatcca gttaaatttg
       61 taaaaatgtg aacctttggt caagaggcaa atcgcgcgga tgaggtagac caatgcgtcg
      121 gtccaggcca tcgcaattgc cacggtcagt aacgcacgga tgagcaagtc cttttgaggg
      181 ccttgccgaa ctagcggaaa cgctgtgacc gccagcacca gtgggatggc aactccttcc
      241 gtcatcacaa tgctccacac gctccacgag gcgcgcgaag atatcgcggc atcctcgact
      301 ccaaccgcca ggcaagccga tgcaacggcc gcgaagacga cgagcttcat catctgcccc
      361 attgtcagtg tcaggcgaaa ctgttgcgaa gctgtagcct cttgggcaga cacgacgttt
      421 ttccccgggg ctaatggcta tcgacgcctt tgaggctcgc cggcaacgac cggggccagt
      481 ttccttgata gctcacgttg cggacccgta cctgcgtctg gtcggcatag tggaacaggc
      541 cgaaggcacg ctggtttaag ggctcgatcg gccgctcgta aatcggctgg ccattgagct
      601 cgagcgtgac cttgtcgccc gtcacgctga ggacgagctg attccaggcc gagggtttta
      661 acggcagcga cgtcgggccg cggcggcatt ctggttcgtc gcgtgcgttg tcggcggtca
      721 ggccgctgcg ctcataggcg ccgtccgtga gcaggtggac cttcacgccg tcaggctcga
      781 gcagaaacgc cagtcggccg agcgccgggt gaaccatcac cttccccgga tcgtggtaaa
      841 attcgtactc gatccggccg tcctcgatca tgggacgatg gtagcgaagc acgctttcct
      901 gcttgctgcc cgtaatatcc tccaaaactc gaccggtgat ttcctcaccg cgcttgtccc
      961 agtcggggtc atcgccaatg accgagtcgc cgtactcggc ggcgagccaa ccgccgaggt
     1021 cgggcagggc ggacagattg actttgtcgg ggatccgcgg atcgccggtg atcgtgaatt
     1081 tgcgcgcagc cccggactgg atgtcctggg ccaagaaagc cacccagggg tcaacatcgg
     1141 gcggagcggg agcttcgtga atcttgcggc cgttaataaa agcggtcatc cgtccccctt
     1201 tgacaacgag gcgatacgga aaccattcgg ccggtttttc gagcggtgga ttaaccgaca
     1261 gctccgccac aggacggccg aactgcgcgc gctccaggtg cttcaaatcg gggttgggac
     1321 cgatggccag gcccccgtac tgcaggcgga tcttgcggtc cggcgagccg gtcagctcgc
     1381 aatcgacctg aaaatcgccg cgcaacggga cagtcaaata cataaggtcg agctcgtggc
     1441 cagggaagtg ggtcaacata tccgcgctcc acccccaatg agaaatcggt tcgcccgcgc
     1501 cgcgtgtatc ggcccgggtt tgcgtgaccc gtgcccatgg caccctatca gggtcggcgc
     1561 caaatggccg tgcagcgccc tcgcccgact tttcg
//
LOCUS       DATAQL010000287         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_639424, whole genome shotgun sequence.
ACCESSION   DATAQL010000287 DATAQL010000000
VERSION     DATAQL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639424"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VHS97_04865"
     CDS             complement(<1..499)
                     /locus_tag="VHS97_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447559.1"
                     /translation="MDSLMHCFGLSRLHAARFWAAGLHDLVSLRQVADTLLALACVWI
                     SVVIFRSYVGRRAPDLPSSKSVWTLGFFLVVCGFSQLASALAMMPVNPLALLSILAAA
                     ACWAAALTLLPLVARVPISQDAEGYKREIVERRRAEAALRSSEAEARKLAEADARKNE
                     FLAMLG"
     gene            932..2056
                     /locus_tag="VHS97_04870"
     CDS             932..2056
                     /locus_tag="VHS97_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447560.1"
                     /translation="MDAKNRAPVWLKLSPTIAIAGLLIVAGCALDKNGSHPGNVVARI
                     GGHSGQLIEPKRCLLKVAIVTRPFGDRTLNEVVWRVADEQIIPPAERRAWEVNGLRVG
                     RIVGELPLELEAILKDTSPQNKVNPTNFFVDSGEPTLIAVGEPVNEASLLLNRENRII
                     GRDYREPSGFFRLTPQHEGPNNVSLRMVPEIHHGPIQRTFQALPNATPLGPQEFRINN
                     GQQEETIREMATSLVLEAGQVAVIGCRPEFKRSLGTFMLTQAVAHSDQRIEKLVMIWA
                     SRNLSGQANEDDERKTSDRPKLFQRLIGPTPTPPVSAPNPPAPDIPQIDTSVPTGAFT
                     KITTGSGASTSGGPPTQAKPTPVPPPNIPTTGSNKSGQSP"
BASE COUNT          478 a          692 c          628 g          374 t
ORIGIN      
        1 gacccagcat tgccagaaac tcgttcttgc gcgcatcggc ctcggcgagt ttgcgcgcct
       61 cggcttcgct gctgcgcagc gcggcttccg ctcgacgccg ctcgacgatt tcgcgtttgt
      121 agccttcggc atcttgcgat atcggcaccc gcgcgaccag tggcagcaag gtgagtgcgg
      181 cagcccagca cgcggccgcc gccaggatcg agagcaatgc gagcggattg accggcatca
      241 tggccagagc gctggcaagc tgtgaaaacc cgcaaacgac cagaaaaaaa ccgagtgtcc
      301 acacactctt ggaagaggga aggtcgggag ctcgacgtcc gacgtacgac ctgaagatca
      361 ccaccgagat ccagacgcaa gcgagcgcga gcaaggtatc ggctacctgt cgcagactga
      421 caaggtcatg gagaccggct gcccaaaacc gcgccgcatg caacctcgag aggccgaagc
      481 aatgcatcag agaatccaag agtatcacct tccttgccac gggtccttcc gcgaccctcg
      541 gatcgtctcg gcgagcgcac cgccgttccg tcaccagact gtgcgtacca cacatatact
      601 tgccgtgagt ctcagtctca agttgcgact gcccgcggat atatttgaac aggatcgacc
      661 gatgtccatt cgacgtaacg tctgatcact tccgggtgtg gctacggcga tttgccgttg
      721 acgagggtgc gggatcgtca tacagtctcg accgatttcc attcaatgcg ccacaggtcg
      781 atgcacgaag caccggccca ttccgagcca ttcaggcggc ggcgggcaag cctggttcgg
      841 cttgccggac tagattagag cgctttccga gcgtttgccg ccggtcaata ggatactgtc
      901 cttcggagta tcgcggaaag gattccgcct catggacgcg aagaatcggg cgccggtctg
      961 gctgaagcta tcgccaacga tcgcaatagc ggggctgctg atcgtcgcgg gctgcgctct
     1021 cgataagaac ggaagccacc ccggcaatgt ggttgcccgc attggtggac acagcggcca
     1081 gctcatcgaa cccaagcgct gtctgctgaa agtcgccatt gtgactcgtc ctttcggtga
     1141 ccggacgctc aatgaagtcg tctggcgcgt cgctgacgag caaatcatcc cgccagcgga
     1201 gcgcagggcc tgggaagtga atggcctgcg cgtgggccgg atcgtcggcg aacttccgct
     1261 cgagctcgaa gcgatcctca aggacacgtc tcctcagaac aaggtcaacc ccactaactt
     1321 tttcgtggac agcggcgagc caacgttgat cgcggtcggc gaaccggtca acgaggccag
     1381 cctgctcttg aaccgcgaaa atcggatcat cggcagggat taccgagaac ccagcggctt
     1441 ttttcgcctg actcctcagc acgaggggcc aaacaacgtg tcgctacgga tggtccccga
     1501 gatccaccac ggcccgattc agcgcacctt ccaggcgctg cccaatgcga cgccgctggg
     1561 tccccaagag tttcggatca acaatggcca gcaagaggaa accatccgcg agatggccac
     1621 gagcctcgtg ctcgaagccg gacaggtcgc cgtcatcggc tgccgccccg agttcaaacg
     1681 cagcctgggg accttcatgc tcactcaggc ggtcgcccac agcgaccagc ggatcgagaa
     1741 acttgtcatg atctgggcgt cgcgcaacct atcaggtcag gcaaacgaag acgacgagcg
     1801 caaaacaagc gaccgaccca agttgttcca acgactcatc gggccgacac caaccccacc
     1861 cgtatccgca ccgaacccac cagcgccgga tattccacaa atcgatacat ccgtgcccac
     1921 tggggcgttt actaaaatca cgaccggctc gggagccagt acgagtggcg gcccgccaac
     1981 ccaggctaag ccaacgcccg taccgccgcc caatatacca acgactggca gtaacaagag
     2041 cggccagtca ccgtgagtgg ggcccgagga tcgattgccg acagctgcag cgcacgactt
     2101 gaactatcct aggcgtgctc actctcggcc gggattgtgg cttttcatca gaggaataac
     2161 gtccatggat gg
//
LOCUS       DATAQL010000288         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3386814, whole genome shotgun sequence.
ACCESSION   DATAQL010000288 DATAQL010000000
VERSION     DATAQL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3386814"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VHS97_04875"
     CDS             complement(<1..243)
                     /locus_tag="VHS97_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX3447561.1"
                     /translation="MPEARLPPQIPSQPGITLSEMAFDEGLAERVRAFVVDDPDVSER
                     KMFGGLCFLSSGNMCFGIVGTELMVRVGPEWYGEALS"
     gene            305..637
                     /locus_tag="VHS97_04880"
     CDS             305..637
                     /locus_tag="VHS97_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447562.1"
                     /translation="MAFGQQQGPPASAKQVRELLELLQEAGHSDFRDARGPMGFTQRQ
                     AGGKFTRDEADALLERLRNAEPGSEKATEVPIWRRSVQEDLLSQLPDERLAVELRRRG
                     WTVVEPLS"
     gene            complement(1110..1703)
                     /locus_tag="VHS97_04885"
     CDS             complement(1110..1703)
                     /locus_tag="VHS97_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX3447563.1"
                     /translation="MDLPELLVADAAEWRSWLSAQHAKSPGVWLVLAKKATTRPTTLS
                     YEEALDEAICFGWIDGRLGQRDSATFRRRFTPRSTRSPWSQRNIAIAERLRAAGRLHR
                     AGEEEVRQAKADGRWKVAYAGQAAMKVPKDLASALKANPAAQAMFQTLTSANRYSILY
                     RIESAKRIETRARRIEQFVGMLARGETLHPQGPRSAQ"
     gene            complement(1862..2686)
                     /locus_tag="VHS97_04890"
     CDS             complement(1862..2686)
                     /locus_tag="VHS97_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX3447564.1"
                     /translation="MIEVPDVVQNKARVAGAQDWLDGLPGLVSSLETEWSITVGCPYG
                     GGSEAFVAEATRADGTPAVLKVLVPGAGNAPSNEATVLLLADGDGCPALYDFDADRGA
                     LLMERLGRPMYELGLPLSRRLELLCTTAERIWRPAPGCGLPTGAEKARWLADFIVNLW
                     EELDHPCSEKTVDHALASARRREHAHRDERAVLVHGDVHQLNALESDDGFKLVDPDGL
                     LAEAEYDLGILMRGDPVELLAGNPFDRALWLAGRTGLEPIPIWEWGVVERLSTALL"
BASE COUNT          524 a          997 c          927 g          560 t
ORIGIN      
        1 gctcaaggcc tcgccgtacc actctggtcc cactcggacc atcagctcgg tgccgacgat
       61 cccgaaacac atgttgccgc tcgagaggaa gcacagcccg ccgaacatct tccgctctga
      121 cacgtccgga tcgtccacca caaacgcccg gactcgctcc gccagaccct catcgaacgc
      181 catctcagaa agcgtaatgc cgggttgcga cgggatctga ggtgggagtc tcgcttccgg
      241 catttacggg catgatcttc ccaccctgat ctgcgcagtc gcaccgaccg gcaagactgc
      301 tccaatggca ttcggacagc agcagggacc accggcgtcg gccaagcaag tgcgggaact
      361 actcgaactt cttcaagaag ccggccacag cgattttcgc gacgcgcgag gccctatggg
      421 gttcactcag cgccaggccg gcggaaagtt cactcgtgac gaggccgatg cactcctgga
      481 gcggttgcgt aacgcagagc cgggctcaga gaaggcgacg gaagtcccaa tatggcggcg
      541 atccgtgcaa gaggacctat tgagccagct gcctgatgag agactcgccg tcgagctgcg
      601 tcgacgcggc tggaccgtag tcgagccatt gtcatagagc cggcgatagg gcagtcgaag
      661 tcgcctcgct acgctctggt ggtgccgatg agcagtactg agaaaatggg atggctccgc
      721 gcatcggtcg gcgttgcctt gatggcagct cctggtgctc ccatccgcct cgccggtacc
      781 gaatcgccga ctgctacggc cctccttctg atgaggacga taggcatccg ggatctcgcg
      841 ctcggtctgg gaaccgttac cgcggcacga tctcatgatc aaggtgattc tcgccgttgg
      901 gtgcaggcca ccttggcgag cgattcgctc gatactttcg tgagtctggt ggcattgagg
      961 tcaatcggaa gacgagactc gttgttcgcc gcggccgtgg cgctcgggtt cgcattcctc
     1021 gacctccgat tgctacagac tgcctccgat ctacggggat agcgacgccc acccggcgac
     1081 gcagacaacc gtcctccttt gcccaccgac tactgggctg atcgtggccc ttgggggtgg
     1141 agtgtctcgc ctcgggccag catgcccaca aactgctcga tccgccgcgc gcgggtctcg
     1201 atcctcttgg cgctctcgat ccgatagagg atggagtacc ggttggcgct tgtgagcgtc
     1261 tggaacatcg cctgagcggc gggattggcc ttcaaggcgc tcgccagatc ctttggaacc
     1321 ttcatggcgg cctgtccggc gtaggcgacc ttccatcggc cgtccgcctt ggcctggcga
     1381 acttcctctt cacctgctcg atgcaatctg ccggcggcac gcaaccgctc tgcgatagcg
     1441 atattccgtt gagaccaagg gctccgagtg cttcgggggg taaatcgccg ccggaatgtt
     1501 gcgctgtcac gctgaccgag ccgcccgtcg atccagccaa agcagatcgc ctcgtcgagt
     1561 gcttcctcgt aggagagcgt ggtcgggcgc gtcgtggcct tcttggctag gaccagccac
     1621 acgcctggtg acttggcatg ctgggcactc aaccagcttc gccattcggc tgcgtcagcc
     1681 accaaaagct ctggaagatc cacgacgtca cggtatcccc gaagcgctgc cgttccgcac
     1741 ggttccggcg atcggggcgg gtctttgacg aacatcgccc gtcctacgaa atcgtcatac
     1801 ccgcgacggc ctcggctgcc cggagcgtct catggccgag aggctgcaga ccgatctgag
     1861 ttcagagcag tgcggtcgac aaccgctcga ccacgcccca ctcccaaatg gggatgggtt
     1921 cgagacccgt ccggccggcc agccaaaggg cccggtcgaa ggggttgccg gccagcaatt
     1981 cgacggggtc tccccgcatg aggatgccca ggtcgtattc ggcctcggca aggagaccgt
     2041 cgggatcaac cagcttgaag ccgtcgtcgc tctcgagagc attcagttgg tgcacatcac
     2101 cgtgcaccaa gacggccctc tcgtcgcggt gtgcgtgttc gcgtcggcgg gcggaggcca
     2161 gggcgtggtc gacagttttc tcggagcatg ggtgatcgag ctcctcccac aagttcacga
     2221 tgaagtcggc gagccaccgg gccttctccg caccggtcgg cagcccgcag cccggcgccg
     2281 gccgccagat ccgctccgcg gtggtgcaca gaagctcgag ccggcgcgag agcggcaggc
     2341 cgagctcata catgggccgc cccagccgct ccataagcag ggcaccccgg tcagcgtcga
     2401 agtcgtagag cgcaggacat ccatccccgt ccgcgaggag gagcacagtc gcctcgttgg
     2461 atggagcgtt tccggcgccg ggaaccagca cctttaggac cgccggagtc ccgtccgccc
     2521 gggtcgcttc ggccacaaac gcctcagacc cgcccccgta cgggcacccc accgtgatcg
     2581 accactccgt ctccaggctc gagacgagcc caggcaaccc gtcgagccag tcttgggcgc
     2641 cggcaacgcg agccttgttt tgcacgacat ccggcacctc gatcaccgtt gcagtctcac
     2701 atcacgccgc tgccggctcc gatagccaga ggttgtggct ggccatccca cacacgatgc
     2761 ggaaatagcg aggtgacgcc gacgcggcgt tgagggatgc tccggtaaat ggagatgtgg
     2821 ggcccggtgc tccttctact ggaccgatcc cttccgggat cgcacctgct gagccttctt
     2881 ttcctgcttc gccgcccgct tctctttgag cgtcttaccg ggcttcttcg cgttgccaga
     2941 tggaggagct ttgtttgcca tcctgcaacg ataggtccgg atgttccgtt gtcgaggcca
     3001 tgctcact
//
LOCUS       DATAQL010000289         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2624473, whole genome shotgun sequence.
ACCESSION   DATAQL010000289 DATAQL010000000
VERSION     DATAQL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624473"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VHS97_04895"
     CDS             complement(<1..470)
                     /locus_tag="VHS97_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX3447565.1"
                     /translation="MRVGIVGFAGSGKSTLFQLLTGTKPDPSKIHAGQVGVATLSDPR
                     LDYLAALHKPKKVTPATVEFLDTPGLLAGSHADNPQRLALIREGDALLIVLNGFTGGD
                     PATQLAAFRDEMLFADLGVVTSRVERLETSVKKARPDRELAQKELEIVKRVQTTL"
     gene            complement(576..>2143)
                     /locus_tag="VHS97_04900"
     CDS             complement(576..>2143)
                     /locus_tag="VHS97_04900"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX3447566.1"
                     /translation="RHLTGFDEEVQLGQLGEILENDTVVMTVEFTDEQHETIRPVGEP
                     LWRGVTMLRYDKGRWHRQAKPTQSVVSFKTDRRRLSKRIHQKINLEPNDSATLFGMRP
                     MLNAQSVHRFAPNLSTNDGTLFRPDSRAGDYDYEVTSDANPAASQPHESPPADGDTLQ
                     LSIPEELREPLRRIALPLVAKIDGEGRDGVLARARALESYLRDSGQFSYTLQMDVKDR
                     RVDPVEDFLVNRKEGHCEYFASALALLLRSVNIPSRVVNGFKGGDWNDLTQTLNVRQK
                     HAHSWVEAYAGLGEDRLPEWITLDPTPSTERQQSIAQVGGLAGNFRPITDVIRHIWVF
                     YIVGFDGERQERLLYTPMRTLLREIRSLYVRLGQSLKSWFSSLFVFQSIGAFISIRGF
                     IVSFVVLTLAAGLANLAFKLAQRFFSWLRGPRLDATSLTAGIIFYRRLTQLLATYELA
                     RNPAETQNEFAGRAHKFLTGKGSPTQPVADVPHEVVDAFYRVRFGHLELEPESLHELD
                     ARLDALESSLKSS"
BASE COUNT          430 a          617 c          657 g          439 t
ORIGIN      
        1 agcgtcgtct gcacccgctt aacgatttcg agctctttct gtgccagttc gcggtctggc
       61 cgagccttct tgacgcttgt ttcgaggcgt tcgacgcggc tggtaaccac cccaagatca
      121 gcaaagagca tttcgtcgcg aaacgccgct aactgggtcg cggggtctcc gccggtgaat
      181 ccgttcaaga caatcagcag ggcgtctccc tcgcggatca gggcgagtcg ctggggattg
      241 tcggcgtgcg atccggccaa gaggccaggc gtatccagaa actcgaccgt ggcaggcgtg
      301 actttcttcg gcttatggag tgctgccaag tagtccagcc gcggatcgga cagtgtggcg
      361 actcccactt gcccagcatg aatcttcgag ggatcgggct tcgttcctgt gagaagctga
      421 aacaaagtgc tcttgccgct gcccgcgaac ccgacgatac cgactcgcat ccttcttcgt
      481 ttccttgctt gtgaaaaatt ccagctcagc tccaggatgg cctgaccgtg ttgttaaacg
      541 atgtgtcacc tgctggagac tccctcagcc ggcggtcagg agctcttcaa gctcgattcg
      601 agggcatcga gtcgcgcatc gagctcatga agcgattcgg gctcgagctc caagtgtccg
      661 aatcgaaccc gatagaacgc atcgacgact tcatgaggga cgtcggcgac cggctgcgtg
      721 ggtgagccct tgccggtcaa gaatttgtgc gcccggccgg cgaactcgtt ctgggtttcg
      781 gccggattgc gagcgagctc gtaagtagcg agcaattgag tgagccgcct gtagaagatg
      841 atcccggccg tcagcgaggt cgcgtccagc ctggggccgc gcagccagga aaagaatcgc
      901 tgggccagct tgaaagcgag gtttgccaga cccgccgcca atgtaaggac cacgaacgag
      961 acaatgaacc cgcgaatact gataaaggcg ccaatcgatt ggaaaacaaa tagactggaa
     1021 aaccagctct tcagtgactg gcccagccgg acgtacagac tgcggatctc gcgcaacaac
     1081 gtacgcatcg gggtgtacag aagacgttcc tggcgttcgc catcgaatcc gacgatgtaa
     1141 aagacccaga tatgacgaat cacatcggtg attgggcgaa agttgccggc cagaccgccg
     1201 acctgagcga tcgactgctg gcgttccgtg gagggggtcg gatcgagggt gatccattcc
     1261 gggagccgat cctcccccag cccggcatag gcctcgaccc agctatgtgc gtgtttctga
     1321 cgcacgttca aggtttgggt caagtcattc cagtcgcctc ccttgaagcc gttgacgacc
     1381 cgtgagggaa tgttgaccga tcggagcaag agcgcaagcg cgctagcgaa atactcgcaa
     1441 tgtccctctt tgcggttgac gagaaagtct tcgactggat cgacacgccg atccttgaca
     1501 tccatctgca aggtgtagct gaactggcct gaatcgcgca gatacgattc gagcgcgcgg
     1561 gcacgcgcca ggaccccgtc gcggccttct ccgtcaattt tggcgaccaa gggtagggcg
     1621 atccgtcgca gtggttctct cagttcctcc ggaatcgaca gttggagcgt gtcgccatcg
     1681 gcaggtggag actcgtgggg ctgggaggcg gccggattag cgtcggaagt gacttcgtaa
     1741 tcgtaatcgc ccgctcgcga atcgggccga aacagcgtgc cgtcgtttgt ggagaggttc
     1801 ggagcgaacc ggtgaaccga ttgcgcattg agcattggcc gcatgccgaa caaggtcgcc
     1861 gagtcgttag gctcgagatt gattttctgg tgtatcctct tgctcaaccg gcggcggtcg
     1921 gtcttgaaac tcaccacgga ctgggtgggt ttcgcctgcc ggtgccagcg tcccttgtcg
     1981 tagcggagca tggtgacgcc ccgccagagc ggctcaccta ccggccgtat cgtctcgtgt
     2041 tgctcgtccg tgaactcgac ggtcatcacg acggtatcgt tttccagaat ctcgccaagc
     2101 tgaccgagct gaacctcctc atcaaaaccg gtcaggtggc ggg
//
LOCUS       DATAQL010000290         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5372156, whole genome shotgun sequence.
ACCESSION   DATAQL010000290 DATAQL010000000
VERSION     DATAQL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5372156"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..>1957
                     /locus_tag="VHS97_04905"
     CDS             38..>1957
                     /locus_tag="VHS97_04905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3447567.1"
                     /translation="MTGCSSDMILAQLLDEQLNEVDHSSIAEHVQTCVRCQERLKELT
                     SQASGVLDWEPLDQLSSDPFITTDHLLSGAPESALLIDHFDPNETGGRSAQVLSESEL
                     PEVDGYEILAELGHGGMGVVYKAYQQRLSRLVALKMIRAGNLAKPEDLARFRIEAEAV
                     AKLRHPNIIQIYDIGEAGNLPFVALELLEGGSLDDFLKRTPQPAKESAQLLATLARAI
                     HAAHQAGIIHRDLKPSNVLFGSDGTPKITDFGLAKRLEDEGYTETGQVLGSPSYIPPE
                     QAQGRAKEVGPAADVYALGAVLYEMLTGRPPFKGTTPVETVMQVLNEDPVPPSYLQSQ
                     VPRDLETICLKCLAKEPHKRYPSAQTLADDLDRYLCDRPIHARPTPLLERGLKWVRRR
                     PTISSLLAVGCLIVSITAARWHEADAARHKTMLESEQVDAALVPVGESVLTKARNDLI
                     DGRFPIEDLTEFLNKISSRPRLADLRSRAFDLLEQARRRYDEKLARDTAKERLREFCR
                     LRDDAYFQDARITSIDHRSTMTAVRETTRLALEIYAAADCPMDEWSMTTLPESLSEQE
                     RDEVSRGCYEMLMVRAEAVAEPLPGESPSGQAHEALLILERARRLSRRPTHAYHLKRA
                     ACLERAGDLEGAQDER"
BASE COUNT          403 a          593 c          595 g          366 t
ORIGIN      
        1 ggaacaacgg ttgacacgga cgcaccgggg aaaagcaatg actggctgct caagcgacat
       61 gattcttgcg caactgctcg atgagcagtt gaacgaggtg gatcattcgt cgatcgctga
      121 gcacgtgcag acctgcgtcc gctgtcagga gcggctgaag gagctcacca gccaggcatc
      181 tggagttctc gattgggaac cgctcgatca attatccagc gaccctttta taaccacgga
      241 tcacctgctc tctggtgctc cggaatccgc acttttgatc gaccattttg acccgaatga
      301 gaccggtggc cgatcggcac aggtcctctc tgaatcggag cttccggagg tcgatggcta
      361 cgagattctg gctgagctcg gtcatggtgg gatgggagtc gtctacaaag cgtaccagca
      421 gcgtttgagc cgccttgtgg cactcaaaat gattcgcgct gggaatctgg cgaaacccga
      481 ggatcttgcg cggtttcgga tcgaggccga ggcggtggcc aagcttcgcc atcccaatat
      541 catccagatt tacgacatcg gcgaggccgg caatctgccg ttcgttgctc tcgaactgct
      601 tgagggaggc agtctagatg acttcttgaa gaggacacct cagcctgcca aggagtcggc
      661 gcagctgctg gcgacactgg ccagggctat ccacgccgcc catcaggcag gcatcatcca
      721 tcgcgacttg aagccgtcca atgtcctgtt tggctccgac ggcaccccca agattaccga
      781 tttcggcctg gccaagcggc tcgaggacga ggggtatacc gagaccggcc aagtactggg
      841 ctcacccagt tacattccgc ccgagcaagc gcaaggacga gccaaggagg tcgggcccgc
      901 cgctgatgtt tatgcgttgg gcgcggtcct ttacgagatg ctcaccggcc gccctccctt
      961 caaagggacc acgccggtcg aaaccgtgat gcaggtcttg aacgaagacc cggtgcctcc
     1021 gtcgtatctc caatcacagg ttccccgcga cctggagacg atctgtttga aatgcctggc
     1081 caaggagccg cacaagcggt atccgagcgc ccagacgctc gcagatgacc tggatcgata
     1141 cctctgcgac cgcccgattc atgcgcggcc gacgcctctc ctggagcggg gactgaaatg
     1201 ggtgcggcgg cggccgacga tttcgagcct gctcgccgtc ggatgtttga tcgtgtcgat
     1261 cacggcggct cgctggcacg aagcagatgc agcccgccat aaaacgatgc tggagagcga
     1321 gcaggtggat gcggcattgg tccctgtggg cgaatccgtc ttgaccaaag ctcgcaatga
     1381 tctgatcgac gggcgttttc ccatcgagga tttgacggaa ttcctcaaca aaatcagttc
     1441 acgaccccgg ctcgccgacc tgcgatctcg cgcatttgac ctcctcgaac aggctcgccg
     1501 ccggtacgac gaaaaactgg ctcgtgatac ggccaaagag cgtcttcggg agttctgcag
     1561 acttcgggac gacgcctact ttcaagacgc gcggatcacc agcatcgacc acaggtccac
     1621 catgacggcg gtgagggaaa cgacccggtt ggcgctcgaa atctacgcgg cggccgactg
     1681 tcccatggac gagtggagca tgacaacact tcccgagtcg ctgagcgaac aggagcggga
     1741 tgaagtatcc cgcggctgct acgagatgct gatggtgcgg gcggaggccg tcgccgaacc
     1801 tctgcccggc gaatccccat ccggtcaggc ccacgaggcg ctcctgatcc tggaacgcgc
     1861 gaggcggctt tcccgccggc ccactcatgc ataccatttg aagcgggcgg cctgccttga
     1921 gcgtgccggc gatctcgagg gagcccaaga cgaacga
//
LOCUS       DATAQL010000291         5917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4456847, whole genome shotgun sequence.
ACCESSION   DATAQL010000291 DATAQL010000000
VERSION     DATAQL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4456847"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..2289
                     /locus_tag="VHS97_04910"
     CDS             205..2289
                     /locus_tag="VHS97_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX3447568.1"
                     /translation="MLIHKLRLTVLALLFLGAIATSAAYLTHSLAMNDEPVKTPAGQL
                     PRVVAGSDQPEPAPVPGPGRMFVTGVVLDPTGKPVARVPVDIIGRPRAPWVATREYVD
                     PRVLVGRGETDAAGRFRLDALRTTADGFFEVYALAAAPGFGLGWVPVNADAKQPSAEI
                     RLRPEQIIRGKLFDVHGQPAAKVELQIWSVGRPNVPQAKGGYDGVSMGNTPPPEGLRV
                     WPRPVTSDDQGRFEITGIGRDVTVGVHVQDQRFTRAGFRVSTDDRDGPKTVTEALRPA
                     MIVEGRVLAADTGLPIPQALVEVGLTMGGAEARCRADNQGRFSAAVQLARNYRMKALP
                     PDGQPYLIGQVEFEWTKGAVKKEVDVKVPRGVVIRGKVIEQGTGRALFGASAQYIPMG
                     NRPNLAMASGSDTTVASAKDGTYQITVRPGKGHLFVFGPKSDYILEAIGGHTIFNGQP
                     GGARHYAHGIIPYEVKAGDQPHEITAVLRPGRTIKGRVVGPNDETVEHAMIISTLHIE
                     RFHLIWRGDLTLHARDGLFELHGVDPEKSTRVSFLDVDHQWGETVEISPKHAGEEVMV
                     RLQPCGQAKARFVGPDGKPVVNVSSQLEILGTPGPHEWDRRKESQSMFAADAASLPSV
                     DRIHYSKSPFTDSEGRITLPALIPGALYRISDRSTVNDRDKGVQVRKDFTVKPGEMLE
                     LGEIMIEKPKAP"
     gene            2413..3780
                     /locus_tag="VHS97_04915"
     CDS             2413..3780
                     /locus_tag="VHS97_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX3447569.1"
                     /translation="MPISINESPWARYVPTPDDPWDLRKVAHLHRRAGFGATWTELLR
                     DREAGPDASIDRLLHPPQPTTEETEAIDAMRRLASHDLLKVCWLHRILYWPDPLREKL
                     ILFWHGHFATSIKKVESVALMDRQNETLRTGALGSFAALLEAVIADPAMLVWLDGGTS
                     KKEKPNENFAREFLELFTMGAGHYTERDVREAARAFTGWARQDSRGRFPDPAVVRDPA
                     QIDDGPKTFLGREGRWGPSDIVRITLERPEAATFLARKLYRWFVSEAGTPDPELIEPL
                     CEELRRNQFATGPIVEVILRSKHFFSRAAYRQRIKSPVEFSAGLVRMLEVPRQALNPL
                     ALAAACDAQGQELFAPPNVEGWVGGSVWINSGTLLERTNWAADIVWGRSENGLTPFDP
                     IAWANRYKLSTDRANLAFLDLLLQGEFSNDARRLAQGAGRDGSPDGLRRALQLLLNCP
                     EFQLA"
     gene            3830..4219
                     /locus_tag="VHS97_04920"
     CDS             3830..4219
                     /locus_tag="VHS97_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3447570.1"
                     /translation="MAILEPPSDLDQTLMALADPTRRAILQRLSQGEARVTELAGPFD
                     MSLNAVSKHIRVLERARLVRRRQAGRDHYLSIDIRPLDRAAAWIESQRLAWNDQLDAL
                     DALLKAEDRSGASRLEIRSPKKKGKRP"
     gene            4216..4650
                     /locus_tag="VHS97_04925"
     CDS             4216..4650
                     /locus_tag="VHS97_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3447571.1"
                     /translation="MSSESTVSVQVTRRFDASAERVFDAWLDPATAGKWLFATKTGQM
                     VRVEIDPCVRGSFTIVDRRDGEDVEHTGEYLEIDRPRRLVFAFRVPKYSQVTTRVSVD
                     IVPLESGCQLTLTHEGVLPDYVSRTESGWGTILDALAETLAS"
     gene            4647..5048
                     /locus_tag="VHS97_04930"
     CDS             4647..5048
                     /locus_tag="VHS97_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX3447572.1"
                     /translation="MTDQNWNPYFRAPWNHCSAKPDAVEDHPWGETVFKIGGKVFAFL
                     GSPDHGGAGMKVAPDQIDGLLALPFVRRSPYIGRYGWLAITIADDDALALAQELIDTS
                     YEMIASKSKGRERPSHGRKGAKKKRTRRKTS"
     gene            complement(5086..>5917)
                     /locus_tag="VHS97_04935"
     CDS             complement(5086..>5917)
                     /locus_tag="VHS97_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447573.1"
                     /translation="GWIQTAPAPAESTPARGCARPQLPQRNHVIADGRLVAYPGAEVV
                     IATEVAGRIIRLPVEEKSVVHRGDLIVELLSDDLQESRDEALARIAEAEADIRFFEQE
                     IKRTHQLRARNAASLFEFQGHERSLDTSRARRKAAIAQKRRFEALIAKTRITSPIDGV
                     ITARFAHPGETIDHAARLVTIADLKRVRIEAEVDEFDISGMALGADVKVSAEGFPHTA
                     WRGKVEEIPDVVVGRRLRPEDPGRPADTRVLLVKIALREPTPLKLGQRIEVEISTTNR
                     "
BASE COUNT         1072 a         1950 c         1881 g         1014 t
ORIGIN      
        1 cgtcgtcgac tcacgcgtcg gggcatcgtc ctgcccgccg ccgccctggc cgcggtactc
       61 gactcccggc cggcctaggc gtcggtttca tcccctctga acgagattac aacccgggcc
      121 gcgctccgct tcgcggccga accggccgcc gtcgatgccc tttcggtctc aactacggcc
      181 ctggcccagg aggtattgag atccatgctg atccacaaac tgcgacttac cgtgttagct
      241 ctgcttttcc tcggtgccat cgccaccagc gcagcgtatc tgacccactc gctggcgatg
      301 aatgacgaac ccgtgaagac cccggcgggc cagctaccgc gcgttgtggc gggatcggac
      361 caaccagagc cggccccggt cccaggccca ggccggatgt ttgtcacggg ggttgtgctc
      421 gatccgaccg gcaaaccggt ggcccgcgtg ccggtcgaca tcatcgggcg gccccgcgcg
      481 ccgtgggttg ccacgcgcga gtacgtcgat ccgcgcgttc tcgttggcag gggcgagacg
      541 gacgccgctg gccgctttcg actcgacgcc ttgcgcacta cggcagacgg cttcttcgaa
      601 gtctacgccc tggccgcggc gcccggattc ggcctgggct gggtgccagt caacgccgat
      661 gccaagcagc catcagccga gatccgactc cgccccgagc aaataatccg cggcaagctg
      721 ttcgatgtgc acggtcaacc cgccgctaag gtcgagctcc aaatctggag cgtgggccgc
      781 cccaacgtgc cccaagccaa gggagggtac gacggcgtga gcatgggcaa tactccgccg
      841 ccggagggac tgcgggtctg gccgaggcca gtcacaagcg acgaccaggg ccgattcgaa
      901 atcactggca tcggtcgcga tgtgaccgtc ggcgttcacg ttcaagacca gcgattcacg
      961 cgcgcggggt ttcgcgttag cacagacgac cgcgatggtc cgaaaaccgt cacggaagcg
     1021 ctccggcccg cgatgattgt cgaggggcga gtcctcgccg ccgacaccgg actgcccatt
     1081 ccccaggcgc tcgtcgaggt cggtttgacg atgggcggag ccgaggctcg gtgtcgagcc
     1141 gacaatcaag gccgcttcag cgctgcggtc cagctcgcca ggaattatcg catgaaggcg
     1201 ttgccacccg atggtcagcc gtacctgatc ggccaagtcg aattcgagtg gactaaaggg
     1261 gccgtcaaaa aggaggtcga cgtcaaagtc ccgcgcgggg tcgtgatccg cggcaaggtc
     1321 atcgagcagg gcaccggccg cgccttgttc ggagcgagcg cgcagtacat acctatgggg
     1381 aaccgcccca acctggccat ggcttcgggt tcggacacga ccgtggccag cgcgaaagat
     1441 ggcacgtacc agatcacagt acggcccggc aaggggcacc tgtttgtctt cggcccgaag
     1501 tccgactaca tcctcgaggc gatcggcggc catacgatct tcaacggtca gccaggtgga
     1561 gcacggcatt atgctcacgg catcatcccc tacgaggtca aggccggcga ccagccacac
     1621 gaaatcaccg cggtgctgcg gccgggcagg accatcaagg gtcgagtggt cgggcccaac
     1681 gacgagactg tcgagcacgc catgattatc tccacgctcc acatcgagcg attccacctt
     1741 atctggcgcg gcgatctcac gctccacgcc cgcgacgggc tcttcgagct gcacggggtc
     1801 gaccccgaga agtccactcg cgtctccttc ctcgacgtcg accaccagtg gggcgagaca
     1861 gtcgagatct cccccaagca cgccggcgag gaggtgatgg tccgtctcca gccgtgcggc
     1921 caggccaagg cgcggtttgt cgggccggat ggcaagcccg tggtgaacgt gtcctctcag
     1981 ttggagatcc tgggaacgcc aggaccgcat gagtgggatc gacgcaagga atctcaatcc
     2041 atgttcgcgg ccgatgcggc atctctgcct agtgtcgatc gcatccatta ctcgaaatct
     2101 ccattcaccg actccgaagg ccgcatcacg ttgcccgctc tgatcccggg tgccttgtac
     2161 cgcatcagcg atcgttcgac ggtcaacgat cgcgacaagg gagtccaggt gcgcaaggac
     2221 ttcacggtca agcccggcga gatgctcgag ctgggcgaga tcatgatcga aaagccgaag
     2281 gcgccatgaa ttgtagggtg ggtcgcgacg cgattgatcg cgtcaatgcc caccgggaga
     2341 gccgcggcac tcggtgggcc tcacgacgcc gagcgtcgtt cgacgccacc ctacgatact
     2401 cggaggcaat ccatgccgat atcaataaac gagtctccgt gggctcgcta cgtgccgacg
     2461 cccgacgatc cctgggacct gcgcaaagtg gcccacctgc atcgccgcgc cggattcgga
     2521 gcgacctgga ccgagttgct ccgcgaccga gaagctgggc cggacgccag catcgatcgc
     2581 ctgctccatc cgccccagcc tacaacggag gagaccgagg ccatcgacgc catgcgccga
     2641 ttggcatccc acgatctgct caaagtctgc tggctgcatc gcatcctcta ctggcccgac
     2701 ccattgcggg aaaagttgat actcttctgg cacggccact tcgcgacgag catcaagaag
     2761 gttgagtcgg tcgcactcat ggaccggcag aacgaaacgc tgagaaccgg tgccctcggc
     2821 agcttcgccg cgctgctcga ggcggtgatc gccgatccgg ccatgctcgt gtggctcgac
     2881 ggtggcacca gcaagaagga gaagccaaac gagaacttcg cccgcgagtt cctcgagctc
     2941 ttcaccatgg gcgccgggca ctataccgag cgggacgttc gcgaggccgc gcgggcgttc
     3001 accggctggg cgcggcagga ctcgcgcggg cgcttcccgg atccggccgt cgtccgtgac
     3061 cccgcgcaga tcgacgacgg gcccaagaca ttcctcggcc gcgaggggcg ttgggggccg
     3121 tcggacatcg tgcggatcac cctcgagcga cccgaagccg cgacgttcct ggcccgcaag
     3181 ctctaccgat ggttcgtcag cgaggccggc acgcccgacc ccgagctcat cgagcccttg
     3241 tgtgaggaac tgcggcgcaa ccagttcgcc accgggccga tcgtggaggt cattctccgc
     3301 tcgaaacact tcttctcccg cgcggcgtat cgccagcgga tcaagtcgcc ggtcgaattc
     3361 agcgccggcc tcgtgcggat gctcgaagtg ccacgccagg cgctcaatcc gctggcgctg
     3421 gcggcggctt gcgacgcgca aggccaggag ctgttcgcgc cgcccaacgt cgagggctgg
     3481 gtaggcggct cggtctggat caacagcggc accctgctcg aacgaactaa ctgggccgcc
     3541 gacatcgtgt ggggccgctc cgagaacggc ctgaccccgt tcgacccgat tgcttgggcg
     3601 aaccgttaca agttgtccac ggaccgtgcc aaccttgcgt tcctcgacct gctgctccag
     3661 ggcgagttca gcaacgacgc gcgccggctg gcgcagggcg ccggccgcga cggcagcccc
     3721 gatgggcttc gcagagcgct ccagctcttg cttaactgcc ccgagttcca actggcttga
     3781 gttgactccg tggccgcctg ccgttattat tcaaccacaa ggttgaataa tggcgatcct
     3841 ggaaccaccg tccgatctcg accagaccct gatggcgctg gccgacccga cgaggcgggc
     3901 gatcttgcag cgcctgtcgc agggcgaagc gcgtgtcacg gagctggcgg ggccgttcga
     3961 catgtccttg aatgcggtct cgaagcacat ccgtgtgctc gagcgtgctc ggctggtgcg
     4021 gcgccggcag gcggggcggg accactacct gtccatcgac atcaggccgc tcgaccgggc
     4081 cgcggcatgg atcgagtcgc agcgtttggc gtggaacgac cagttggacg ccctcgacgc
     4141 cttgctcaag gcggaagacc ggtcgggtgc gtcacgtctt gaaataagat ctcccaagaa
     4201 gaaaggaaag cggccatgag cagcgaatca accgtcagcg ttcaggtcac ccgtcgtttc
     4261 gacgcctccg cggagcgcgt cttcgacgcc tggctcgatc ccgccacggc aggcaagtgg
     4321 ctgttcgcaa cgaaaacggg ccagatggtc cgcgtggaga tcgacccgtg cgtcaggggg
     4381 tcgttcacca tcgtcgatcg tcgcgacggc gaagatgtcg agcacaccgg cgaatatctg
     4441 gagatcgacc ggccgcgacg gctcgtgttc gcgttccgcg tcccgaaata ctcgcaagtg
     4501 accacccgcg tttcggtcga tatcgttccg ctcgagagcg gatgccagct caccctgacc
     4561 cacgagggcg tgctgccaga ttacgtgagc cgcaccgagt ccggctgggg gacaatcctc
     4621 gatgctctcg cggagacgct tgcatcatga ctgatcaaaa ctggaatccc tacttccgcg
     4681 cgccctggaa ccattgttcg gcgaagccgg atgcggtgga agaccacccg tggggcgaga
     4741 ccgtcttcaa gatcggaggc aaggtcttcg cctttctcgg ctccccggat cacggcgggg
     4801 ctgggatgaa ggttgccccc gaccagattg atggcctgct cgccttgccg ttcgtccggc
     4861 gatcgccgta catcggccgt tatgggtggc tcgccatcac catagcggac gacgacgcat
     4921 tggcattggc tcaagaattg atcgacacaa gttacgagat gattgcctcg aaatcgaagg
     4981 gccgcgaaag gccgagccat ggccgaaagg gcgctaagaa gaagcgaacg aggaggaaaa
     5041 cgtcttgatc cgcttcattc acttctggtt cccatgccag ccgggtcatc tgttcgtcgt
     5101 ggagatttcc acttcgatcc gctggcccag tttgaggggt gtcggttcgc ggagggcgat
     5161 tttcaccaga aggacccgcg tatcggcagg acgcccggga tcctcgggcc ggagtcgccg
     5221 gccgacaacg acgtcgggga tctcctcgac tttcccccgc caggccgtgt gcgggaaacc
     5281 ctcggcggaa accttgacat cggcgcccaa ggccatgccg ctgatgtcga attcatcaac
     5341 ttcggcctcg atccgaaccc gcttcaggtc ggcgatggtg accagacgcg cggcatggtc
     5401 gatcgtttca ccgggatgcg cgaaccgagc ggtgatcacg ccatcgatcg gcgaggtgat
     5461 gcgcgttttg gcgatcaagg cctcaaagcg gcgcttctga gcaatcgccg ccttgcgccg
     5521 tgcgcgtgag gtatcgagac ttcgctcgtg accttggaac tcgaacaaac tggcggcgtt
     5581 gcgggcccga agctgatgag tgcgcttgat ttcctgctcg aaaaagcgga tatcagcctc
     5641 ggcttcggca atccgcgcga gcgcctcgtc gcgcgactct tgcagatcgt cggacagcag
     5701 ctcgacgatg agatcgcccc gatgcacaac cgacttctcc tcgacgggca agcggatgat
     5761 tctcccggcg acttcggtcg cgatcacgac ctcggcgccg gggtaggcaa ctagcctgcc
     5821 gtcggcgatc acatgatttc gctgcggcag ctgcggcctg gcgcacccgc gcgccggcgt
     5881 gctctccgcc ggcgctggcg ccgtttgaat ccagcca
//
LOCUS       DATAQL010000292         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1555625, whole genome shotgun sequence.
ACCESSION   DATAQL010000292 DATAQL010000000
VERSION     DATAQL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555625"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VHS97_04940"
     CDS             <1..1062
                     /locus_tag="VHS97_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447574.1"
                     /translation="GSEALVEAREILLQRGSTPRVYRNMLVFLAAEARQLDNLKDAVR
                     SALAWAEIVKEKDRLNLTQNDDALAKVKLAEANETVKTRLKEAWCYLHYPVQESALAE
                     VEWISGKVPAQDGLLARASKKLVADEGLMPELGPTRLDRDLRKYIWNDKPHLSLKDLW
                     EYLNRYTYLPRLKNQAVLVKTVQAAIGGMFPGPFAYAERWDENAQTYLGLAIERAPNA
                     AVVIDSDSVILKLDVAETHRPKPTAPSDRPGVEEAPSRDGAPSPGRPTEGAPATPTQE
                     KKPTRFLGTVMISSERPAREIHQVIEAIVEQLTTIPGSEVSLKLEIDAEVPAGLDRAK
                     VRTLIENANTLGFIDKSIS"
     gene            complement(1153..1620)
                     /locus_tag="VHS97_04945"
     CDS             complement(1153..1620)
                     /locus_tag="VHS97_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447575.1"
                     /translation="MPASIGYADGGQLFFEHPQYFTSHRRSGLYTALLFPLLLARRAT
                     QFLPGSWVGLLVSPPDEDGNGYVELACAGYTRQPVGGLCVSGAANAIAIEFPPLVDIV
                     ESVSHVAIFYPNGRMSHCGELALKQASESEACVFRFPAGALVIDLSRHHTRTN"
     gene            complement(1822..>2102)
                     /locus_tag="VHS97_04950"
     CDS             complement(1822..>2102)
                     /locus_tag="VHS97_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3447576.1"
                     /translation="RPGDIVILDNLGSHRRRQVRRAIRHAGARLLFLPKYSPDLNPIE
                     QVFAKLKHLLRKAQARSLEALLAAIPKLLEAFEPAECANYLKNSGYAA"
BASE COUNT          422 a          613 c          708 g          359 t
ORIGIN      
        1 ggctctgaag ccctggtcga ggccagagag atcctgcttc agcggggcag cacgccgcgt
       61 gtctatcgca acatgttggt ctttctcgcg gcggaagccc gccaactgga caatctcaaa
      121 gacgccgtcc gctcggccct cgcctgggcc gagatcgtca aggagaaaga ccggctgaat
      181 ctcacccaga acgacgacgc attggcgaaa gtgaagcttg ccgaagcgaa cgagaccgtg
      241 aagacccggc tgaaggaagc gtggtgctat ctgcactatc ccgttcagga gagcgccctg
      301 gccgaggtcg agtggatctc aggtaaggtc ccggcccagg acggcctcct cgctcgggcg
      361 agcaagaagc tcgtcgcaga cgaagggctg atgccggagc ttggccccac gcgcctggac
      421 cgcgacctcc ggaagtacat ctggaacgac aagccgcacc tgtcgctcaa ggacctctgg
      481 gagtacctga accggtacac ctacctgccg cgactgaaga accaagccgt gctggtgaag
      541 acggtgcagg cggcgatcgg gggaatgttc cctggcccgt tcgcctatgc cgaacgttgg
      601 gacgagaatg cgcaaacgta cctcggcctc gctatcgaac gcgcgcccaa cgccgctgtt
      661 gtgatcgaca gcgacagcgt gatcctcaag ctggatgtcg ctgagacgca tcgtcccaag
      721 ccgacggcgc cgagtgatcg cccaggtgtt gaggaagcgc caagtcgtga tggcgctccg
      781 tctcctggtc ggccgacgga gggagcccct gcgacaccga cgcaggagaa gaagccgacc
      841 cgcttcctcg gcacggtgat gatctcgtcc gagcgcccgg ctcgcgaaat ccatcaggtc
      901 atcgaagcca tcgttgagca actgacgacg atcccgggca gcgaggtgtc gctaaagctg
      961 gagatcgacg ccgaagtgcc ggcaggcctc gaccgcgcca aggtgcgcac cttgatcgag
     1021 aacgccaaca cgctcggctt catcgacaag tcgatcagtt agagtgcgcg ctgatgggcg
     1081 cgcgggcatg cgatggattg cccgatgcgc cgttgcgctg gcgacgcact cggccgaccg
     1141 acagcggctc cgttagttcg tccgcgtgtg atgtcgggaa aggtcgatca cgagcgcacc
     1201 ggcggggaac ctgaagacgc aggcttcact ttcactagct tgcttgagcg caagctctcc
     1261 acagtgggac atgcggccat tgggatagaa gatggccacg tgactgacgc tttctacgat
     1321 gtcgacgaga ggcggaaatt cgatggcgat cgcgtttgcg gcgccggaga cgcacaagcc
     1381 acctaccggt tgcctggtat agccggcgca cgccagttca acgtagccgt tgccatcctc
     1441 gtccggaggg ctgaccagga gtcctaccca agagcccggc aagaactggg tggcgcgtcg
     1501 ggccaggagc agcggaaaca acagagccgt atagagacca ctgcggcgat gggaggtgaa
     1561 gtattgcgga tgctcgaaga agagctgtcc gccgtcggca tagccgatcg aggcaggcat
     1621 tgattagttc tcgatttaag ggagcccgca atgagcggga cgatgccgga agacggcccc
     1681 cggcactcct aatgcttgcc acagacggcc cgtaaagtca agatttcgtc gagcttattt
     1741 gagatcgccg acctcgtggc ggctctgctg caagacgggg ctttggggtc agccctgatc
     1801 gcgccctaga gcgtgttgag tttaagcggc atagcctgag tttttgaggt agttggcgca
     1861 ttcggctggc tcgaaggcct cgagcagttt ggggatggcg gcgaggaggg cttcgaggga
     1921 gcgggcctgg gccttgcgca agagatgctt gagcttggcg aagacctgtt cgatggggtt
     1981 cagatccggc gagtacttag gcaggaagag cagcctggcg ccggcgtgtc tgatggcgcg
     2041 gcggacctgc cggcggcggt ggctgccgag gttgtccaag atcacgatgt cgccggggcg
     2101 ca
//
LOCUS       DATAQL010000293         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1861152, whole genome shotgun sequence.
ACCESSION   DATAQL010000293 DATAQL010000000
VERSION     DATAQL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861152"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..1025
                     /locus_tag="VHS97_04955"
     CDS             495..1025
                     /locus_tag="VHS97_04955"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX3447577.1"
                     /translation="MVASAADPTKKAASVLDFHAKDIEGKDVDLASYKGKVLLIVNTA
                     SQCGLTPQYKDLEAIYEKYKGQGLEILAFPANEFGGQEPGSNDEIKEFCSTKYKVSFP
                     LFSKVVVKGKGIDPLFDFLTSDATNPKFAGPIKWNFNKFLVNRKGEVIARFEPKKDQH
                     WSDPVVVAIEKALVEK"
     gene            complement(1158..>2964)
                     /locus_tag="VHS97_04960"
     CDS             complement(1158..>2964)
                     /locus_tag="VHS97_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447578.1"
                     /translation="QPLVDDKASGLLGPESTASPLTAVAKATPKGKDLAPALPPPEPR
                     SEMAGAESANPRAQPAILPPSLADGDQVASTDAKTAAESEQVSENSIGQSSSSHSSAL
                     TSDRRLRPDRIAVNPPQGAGTPLAATDKPVSIGPPDAGGTSGTERRRILPEIEGMARP
                     REAIPLVRATPNSRVANAGASGLAALGDRVGGQNPTADIPRIYQPRLEPDRSTRAQRI
                     GASAASELAVERALDWLARHQDDDGRWDGGIARYEDGSPVKGDDSFTVHCPPGETCFG
                     DCAYWEADTALTALALLTYLGAGYTHNDGRYTEVVGKGLDFLIEQQKPDGDLRGRSKV
                     VGMYCHAMATLALCEACALTADDKLRRPAERAVAFLVNARARDGLAWRYAPGAPVGDT
                     SILGWVVMGLKSAKEVGIRIPAESTVRRGTLTWLDKVATGQSKGLARYQPWEPVTPTM
                     TAEAWVCRQFLGVGGPGPSSSEAAQFLLENDSDRGTSNVYYWYYATLALYQHGGEPWS
                     KWNAKIRDRIVGLQSTAGHQTGSWEPDNSLYGAKGGRIYCTTLAALSLEVYYRYLRLY
                     DAPMIPLEAGEAPARRDSNPERSSSATAASRNENR"
BASE COUNT          546 a          943 c          875 g          600 t
ORIGIN      
        1 ggcacgggtc tccggctcgg acgcgagtgc agcgagcaag tcgagaggct caacgactga
       61 cccacggcga cgttttgcca gcagatcggc tcgcaacatg gctcgctcgg cactcgccgt
      121 aaacgactcg aatatcactc ccgtaactcc ctgattgcac tcatggaatt aatacggccg
      181 accaaagccc acaccgaggc ctcttgctac ccgaatcggt gatttcgacg gcctaccgca
      241 aggctcccga ggcgatgtca ctcttgaatt ttcggccgca caatcccatg ataaatgtct
      301 gaacttcacg accctctgct taccgccggc ttcgtttcag ccgcttgggc atttgtcgtg
      361 tacgccccaa cgcggcaggc cgtttcttcc tgccccaata gcctcacaca caaaggactc
      421 tcaccgtgtc tcaccggtcg ttgctgttac tatccctcgc gagtctgacg ttcctgctac
      481 ttgccgctcc cctcatggtc gcgtcggccg ctgaccctac gaaaaaagcg gcgtccgtcc
      541 tggattttca cgccaaggac atcgaaggca aggatgtgga tctggccagc tataaaggca
      601 aggtcttgtt gatcgtgaat acggccagcc agtgtgggct tactccacaa tacaaagacc
      661 tggaagccat ctacgaaaaa tacaagggtc aaggcttgga gattcttgcc tttcccgcca
      721 acgagttcgg cggtcaagaa ccaggctcca acgacgagat caaggaattc tgctccacca
      781 agtacaaagt cagctttcct ctgttctcca aagtcgtcgt caaaggcaag gggatcgatc
      841 cactttttga cttcctcacc agcgatgcga ccaaccccaa gttcgccggt ccgatcaaat
      901 ggaactttaa caagttcctg gtcaatcgaa agggtgaggt gatcgcccgc ttcgagccga
      961 agaaagacca gcactggtcg gaccccgtcg tcgtcgcgat cgaaaaggcc ctggtcgaga
     1021 agtaattcac ccttcgcgga caacatcgtc cacacggggc gaggcgaccg ttggtttcgc
     1081 tccatccatg gggccgaatc gcaaatgtgt gcccgccccg ttcgctccgg agagaagatt
     1141 gcagggcggg tgacctttca tcgattctcg ttgcgacttg cggccgtggc ggaagaagac
     1201 cgctcggggt tgctgtctcg tcgcgctgga gcctctcctg cttcgagtgg aatcataggc
     1261 gcgtcgtaga gccggagata gcggtaatag acctcgagag acagggccgc gagcgtcgtg
     1321 caatagatcc ggccaccctt ggctccataa aggctgttgt caggctccca gcttccagtc
     1381 tgatggcccg cggtcgattg aagccccacg atccggtcgc ggatcttggc gttccatttt
     1441 gaccacggtt cgccgccgtg ctggtagagg gccagggtcg cataatacca gtaatagacg
     1501 ttcgacgtgc cgcggtccga gtcgttttca aggaggaatt gggccgcctc ggaactggac
     1561 ggaccgggcc cgcccacccc caggaactgc cggcagaccc acgcttcggc tgtcattgtc
     1621 ggggtgaccg gctcccacgg ttgataacgg gccagtccct tggactggcc ggtcgcaacc
     1681 ttgtcgagcc atgtcaaggt gccgcgccgg accgtggatt cggcggggat tctgatgcca
     1741 acttccttgg cggacttcaa tcccatcacg acccaaccga ggatgctcgt gtcccccacg
     1801 ggcgcacctg gagcgtaccg ccaggccaga ccgtcacggg ctcgagcgtt taccaagaat
     1861 gcgaccgcac gctccgcggg acgacgcaac ttatcgtcgg ccgtaagcgc acaagcttcg
     1921 cataatgcca gcgtagccat cgcatgacag tacatgccca cgaccttact gcgaccacgg
     1981 aggtccccgt ccggcttctg ctgttcgatc aaaaagtcca ggcccttgcc gaccacctca
     2041 gtgtaccggc cgtcgttgtg cgtgtaaccg gctccgagat aggtcaagag agccagcgcc
     2101 gtcagggccg tatctgcctc ccagtaggcg cagtctccaa agcaagtctc gcctggtggg
     2161 caatgcaccg tgaagctgtc gtcgcccttt acgggcgacc cgtcttcgta tcgggcgatg
     2221 ccgccatccc aacggccgtc gtcatcctgg tggcgcgcga gccagtcgag tgcgcgctcg
     2281 actgccaatt cgcttgcggc gctggcgccg attcgctggg cccgtgtcga acggtcgggt
     2341 tcaagcctcg gttgataaat ccttggtata tcggctgtcg ggttctggcc gccaacacga
     2401 tcacccagcg ccgccaggcc ggacgcgccg gcattcgcca cgcgggagtt gggggtcgcc
     2461 cgcaccagcg gtatcgcctc gcgcggacgc gccatcccct cgatctcagg gaggattcgg
     2521 cgtctttcgg ttcccgaagt tccaccagcg tcgggggggc cgatgctcac gggcttatcg
     2581 gtggccgcca acggcgtgcc ggcgccctgc ggtgggttca cggcgattcg atccgggcgt
     2641 agccgcctgt cgctcgtcag cgcggacgag tggcttgatg atgattgccc aatcgagttt
     2701 tcactgacct gttccgattc ggcggccgtt ttggcatccg tggaggcaac ttggtctccg
     2761 tcggccaagc ttggtggtag tatagctggt tgcgcccgtg gattggcgct ctccgccccg
     2821 gccatctcgg agcgcggctc tggcggcggt agcgccggcg cgagatcctt gcctttggga
     2881 gtcgctttcg ccaccgccgt caacggcgag gccgttgatt cagggccgag gagccctgat
     2941 gctttgtcgt cgaccagcgg ctgg
//
LOCUS       DATAQL010000294         6033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2167014, whole genome shotgun sequence.
ACCESSION   DATAQL010000294 DATAQL010000000
VERSION     DATAQL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167014"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1020
                     /locus_tag="VHS97_04965"
     CDS             295..1020
                     /locus_tag="VHS97_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447579.1"
                     /translation="MPGNLARKRHEARQTVDELRRHLGLFSYQRDGQTAESGQQVGLS
                     TQLGVRPGGIVELLLEKEGAGAVTLALQIIVQSSGGRGVWAIVDPARECYVAALSGWG
                     IQPARTLVLRPGTLQETCWAIEQCLRCPGVSTTLAWLEQRIPARVHRRWQLAAEVGGG
                     IGLFFRPVWARREPIWADSRLLVTPMAGGEGESRRIHIEVLYRRGGFGGSGQAWEIDH
                     ATGHVRLVSPMANPAAAAREARA"
     gene            947..2455
                     /locus_tag="VHS97_04970"
     CDS             947..2455
                     /locus_tag="VHS97_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX3447580.1"
                     /translation="MPRVMCVWFPQWPIQRLRHARPELNRSELVLFAGLHQRPAVIVC
                     TAKAEKMGVYSGQPLAEAKALLPRALFLPADLAGDRVALCQLALDFQQFSPFVGLEEG
                     AYPESLLSDVTGCTHLWNGEERFLRSVRNYWRKRGYQTQLALTGTLGSAWALAHSTTC
                     SLVPAGNEEMALSALSVTTLRLPVSVLEPLEALGLVTIGDVLALPRETLASRFGMILP
                     QRLDQVLGHLTETFVCERLKEPITSIREWEAPIDDRFILGLACREMVRELLSLTERHG
                     MGIQELEGELQTENGRITIEIKLVEPTRDDGHLAQLIELQLERQTWSGGIIAVRWAAL
                     RLGRLEQTQRSWFQEECETKNSRLFNTLVERLSSRLEASAILRVEFVADSQPEHVVRL
                     VPWTKTKPPETDVVVPSPEQSRGRPCRLFGSPVPIEVASLVPDGPPYLMVWQREDCRV
                     VRSWGPERIATGWWRAEDVERDYYRAEWEDGTQVWVYHDKRNGRWFLHGFFD"
     gene            2462..5740
                     /locus_tag="VHS97_04975"
     CDS             2462..5740
                     /locus_tag="VHS97_04975"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX3447581.1"
                     /translation="MPEQPHSKLHPQAPVRYLTAAATPPVPYVELHCKTNFSFLEGAS
                     HPSELVVQASMLGYRGLAVTDRNSLAGVVRAHVAAKETDLKLIVGAEVTLVDASPILL
                     WAMNRDGYGRLSRLLTRGRRQAPKGECRLSFTDVVEHASGLLAGLLLSQVGDLNCLAR
                     FGEGEPPGEPHAKTARTDFGELSRAEPRRPRITKRHLESELTRWREVFPDRIYAVAEL
                     HRGLDDRRRLERWRCAARAARVPLIAAGDVHYHDPSRRYLQDVLTAIRTKTTVAELGR
                     VRFPNGERRLRAVDEILTLFDQCPEVVERTSEVAQRCSFSLEELRYEYPEELCPASET
                     PDSYLARLTWAGALERYPGGVPTKVSELIERELAIIEELNYAAYFLTVWDLVRFARAR
                     EILCQGRGSAANSAVCYCLGVTAVDPDRIDVLFERFISKDRAEAPDIDIDFEHQRREE
                     VIQYIYEKYGRERAGMTAELITYRPRSAVRDIGKALGLSLDRLDALANAIGHVSEGDE
                     LILRLAEAGLDPTSRLGRQLIHLVAEILGFPRHLSQHVGGMVLTQLPLCEIVPIENAS
                     MPDRTVVQWDKDDLDALGILKVDCLALGMLSAIRRSLDLLKEHAGLPLTLATIPAEDP
                     SVYEMIQKADTVGVFQIESRAQMSMLPRLKPNKFYDLVIEVAIVRPGPIQGKMVHPYL
                     RRRNGDEPVKYPNEAVREVLEKTLGVPLFQEQAMKLVVVAAGFTPGEADKLRRAMAAW
                     KRTGAISQFEEQLISGMLANGYSEEFARNLFRQIEGFGSYGFPESHAASFALLVYVSA
                     WIKCHYPAVFLASLLNSQPMGFYGPAQLVADARRHGVEVRPVDVNHSDCDSTLERSDS
                     DGTLAVRLGWSTVKGLSTTAAERVVCERRAGLFTSYGDFVARTGLSAAVLSRLAAADA
                     FRSLALSRRPALWMSLAMTPPEPLLAALPDEAPPPLPRLSPAEEVIHDYHAQGLSLRG
                     HPLAPLRPFLDKDRVVPASALESVEPDRRYRVAGLVLLRQRPGTAKGVTFMTLEDETG
                     NVNLIVWPQVWQRYRRVARQARALIATGLLQRQDGVIHVIVDRLEDLTTRLPDLGHVS
                     RDFH"
BASE COUNT         1171 a         1727 c         1852 g         1283 t
ORIGIN      
        1 cacgggtgtt ggaggacgac cccgatcgtc gcgaacagcg cgttcacata acgtggggca
       61 ctcatgcggt tgccggtctt cgacaccacg atcttgaagt cgtttctgag cgcgctctgg
      121 ctcacaccat tggccaactg ctcgagggcg tagctaagga ctagaacacg cactttgggc
      181 agtcagggac atgtttcatt tgatctccat gacttccgaa gaaggcgatt gacttctacc
      241 tgtacagaag gttagtatct cgagcgagtc acttgccgct gtcaggagat gcacatgcca
      301 ggaaatcttg cccgcaagcg acatgaagcc cggcagactg tcgatgagct acgtcgacat
      361 ctcggattat tttcgtatca gcgagatggg cagactgctg aatccggcca gcaagtcggc
      421 ttatcgactc agcttggtgt gcgacctggg ggaattgtgg aactgctgct ggagaaagag
      481 ggagcgggag ctgttacgct cgccttgcaa atcatagtgc aatcctccgg cggccgagga
      541 gtctgggcga ttgtggatcc tgcgcgggaa tgctacgtcg ctgctctatc aggatgggga
      601 atccaaccag cgagaacact cgttctccgg ccgggcactc ttcaggaaac atgctgggca
      661 atcgagcaat gtctgcgttg tccgggtgtg tcgacgacat tggcatggct cgaacagcgg
      721 atccccgcac gggttcatcg tcgctggcaa ctggcggcag aggtgggagg aggcattggc
      781 ttattttttc ggccggtctg ggcgcgacgg gaacccatct gggccgattc acggttgctg
      841 gtgacgccga tggccggagg agagggagaa tcgagacgga ttcatatcga ggttctgtat
      901 cgccgtggcg gctttggagg aagcggccag gcctgggaga tcgatcatgc cacgggtcat
      961 gtgcgtctgg tttccccaat ggccaatcca gcggctgcgg cacgcgaggc ccgagcttaa
     1021 tcggtcggag cttgttctgt tcgccggcct tcaccagcgt cctgcggtca ttgtttgcac
     1081 cgctaaggcc gaaaagatgg gcgtttatag cggtcagccg ctggccgagg ccaaagcgct
     1141 tttacccaga gctctgtttc ttccagcgga tcttgccggg gaccgagttg ctctctgtca
     1201 actggcgctc gactttcagc aattctctcc cttcgtgggt ctcgaagagg gagcatatcc
     1261 cgaatccttg ctcagcgacg tcaccgggtg tacccatctt tggaacggag aggaaaggtt
     1321 tcttcgatcc gttcggaact actggcgtaa gcgaggctat caaacccagc ttgcattgac
     1381 cggcacactg ggatctgcct gggctctcgc tcattccacg acgtgctcat tggtgccggc
     1441 agggaatgag gaaatggcat tgtcggccct ttccgtgacg acgctgcgtc tgccggtttc
     1501 ggttctcgag cccctggaag ctcttggact cgtgacgata ggcgatgtgc tcgcactgcc
     1561 acgcgaaacg ctcgccagcc ggtttggaat gatcttgccg cagcggctgg accaagtcct
     1621 gggtcatctg acggaaacgt tcgtctgcga acgcctcaag gaaccgataa cctcgattcg
     1681 cgagtgggaa gcgccgattg acgaccgttt tatcctggga cttgcgtgcc gggaaatggt
     1741 gcgtgagctg ctgtcgctga ccgagcgtca cggcatgggt atccaggagc tcgaaggaga
     1801 gctccagacc gaaaatggtc gcatcaccat cgaaatcaag cttgtggaac cgactcgcga
     1861 cgacgggcat ctcgctcagc ttatcgaact gcaactggag cgacagacgt ggtccggcgg
     1921 cattatcgcg gtacgctggg ctgctctccg actgggacgc ctcgagcaaa ctcagcgatc
     1981 ttggtttcag gaggaatgcg agacgaaaaa ctcgcgtttg ttcaatacat tggttgagcg
     2041 tctcagcagc cgactcgagg ccagtgcgat cttgcgagtc gagttcgttg ccgattcaca
     2101 gcctgagcac gtcgtccggc tggtgccttg gaccaagacg aaaccgccgg agactgatgt
     2161 ggtcgtgcca tcccctgaac aatcgcgcgg acgtccttgc cgactgttcg gcagcccagt
     2221 gccgatcgaa gtggcatcgc tcgttccgga cggtccgccc tacctcatgg tgtggcaaag
     2281 agaagactgt cgcgtcgtcc gctcatgggg gccggaacgg atcgcgacgg gttggtggcg
     2341 cgcggaggac gtcgagcgcg actattaccg cgcggaatgg gaagacggca cacaggtctg
     2401 ggtgtatcac gacaagcgca atggtcgctg gtttctccac ggctttttcg actgaacgat
     2461 catgccagaa cagccgcatt ccaaactcca tcctcaagcg cccgtacgat acctgactgc
     2521 cgcggccaca ccgccggtcc cttatgttga gcttcattgc aagacaaact tttctttttt
     2581 ggaaggtgcg tcccatccca gcgagctggt cgtccaggcg tccatgcttg gctacagagg
     2641 actggcggtc accgatcgaa acagcttggc tggcgtggtc cgtgctcatg tcgccgccaa
     2701 ggaaacggat ctcaagctga tcgtcggtgc ggaagtgact cttgtcgacg ccagcccgat
     2761 tttgctgtgg gcaatgaatc gcgatgggta tggccggctc agtcgactct tgactcgcgg
     2821 ccgtcgccaa gctcccaagg gagaatgccg actctccttc acggacgtcg tcgagcacgc
     2881 aagcggtctg ctcgcgggtc tcttgctgag tcaggtcgga gacctaaatt gccttgcacg
     2941 attcggggag ggcgagcctc ccggcgagcc acacgccaaa acggctcgga cggacttcgg
     3001 cgagctcagt cgagccgagc ctcgccgtcc cagaatcacg aaacgtcatt tagaatcgga
     3061 attaacgcgg tggagagaag tttttccgga tcgcatttac gcggtggccg agctccatcg
     3121 tggccttgac gatagacgac gattggaacg atggagatgt gccgcccgag ctgctcgcgt
     3181 gccactgatc gccgcgggag acgttcacta ccacgacccc agcaggcgct atcttcaaga
     3241 tgtactgacg gcgattcgca cgaagacgac ggtggccgaa ctaggccgcg ttcgctttcc
     3301 caatggagaa cgaaggctga gagcagtcga tgagatcctg actctgttcg accagtgccc
     3361 tgaggtcgtt gaacgcacct ccgaagtagc ccagcgctgc tcgttctcac tcgaagaatt
     3421 gcgctatgaa tatccggaag aattatgccc ggccagcgag acacccgatt cctacctggc
     3481 gcggctcaca tgggccggag ctttagagcg ttaccccggc ggtgttccga caaaggtgag
     3541 tgagctcatc gagcgcgagc tggccatcat cgaggagttg aactacgcag cctattttct
     3601 caccgtctgg gacctggttc gctttgctcg agcccgtgag attctctgcc aggggcgcgg
     3661 gtcggcggcc aactcggccg tttgctactg cctgggggtc acggccgttg accccgatcg
     3721 catcgatgtc ctctttgagc ggtttatttc caaggatcgg gccgaggcgc ccgatatcga
     3781 catcgatttc gagcatcagc gtagagaaga agtgattcaa tatatctacg agaaatatgg
     3841 ccgtgaacgg gccgggatga cggcggagtt gatcacttat cggccgaggt ccgccgtgcg
     3901 cgacatcggc aaggcgcttg gcctgtcgct cgaccgtctc gacgcgctgg caaacgcgat
     3961 cggtcatgtc agcgagggag acgagctgat tcttcgcctg gcggaagctg gacttgaccc
     4021 caccagccgc ctgggccggc agttgattca tctcgtcgct gaaatcctgg gatttccccg
     4081 ccatttatca cagcatgtcg gcggcatggt tctgacccaa ttgccgttgt gtgaaatcgt
     4141 gccgatcgaa aacgcgagta tgcccgaccg aaccgtcgtg caatgggaca aagatgacct
     4201 ggacgccctc ggcatcctca aggtcgattg ccttgcgctt ggaatgctct cggccataag
     4261 acgcagtctg gatctactca aggagcacgc cggtctaccg ttgactctgg ccaccattcc
     4321 ggccgaagac ccgtctgtat acgagatgat tcaaaaggca gacacggtcg gtgttttcca
     4381 gatcgagtcg cgcgcccaga tgagcatgct gccgcggctg aaaccaaaca agttttatga
     4441 cctggtcatc gaggtggcaa ttgtccgccc cggcccgatt caggggaaga tggttcaccc
     4501 ctacctgcgc cggcgcaacg gcgatgaacc ggtcaaatac cccaacgaag ccgtgcgcga
     4561 ggtactggag aagaccctcg gcgtgccact atttcaagag caggccatga agctcgtggt
     4621 tgtggcggcg ggtttcactc cgggtgaagc tgacaagttg cggcgtgcga tggcggcctg
     4681 gaagcggact ggagccatca gtcaattcga ggagcaatta atcagcggca tgctggctaa
     4741 cggttactcg gaggagtttg cccgcaacct attcaggcag atcgaaggct tcggctcgta
     4801 tggctttccg gaatctcatg cggcgtcgtt tgccctcttg gtttatgtct cggcctggat
     4861 caagtgccat tatcccgcgg ttttcctggc ctccctcttg aatagccagc cgatgggttt
     4921 ctatggcccg gctcagttgg tcgccgacgc gcgccggcat ggagtcgaag tccgtccggt
     4981 tgacgtgaac cacagcgatt gtgacagcac gctggagcga agcgattctg acggcacgtt
     5041 ggcggtgcgg ctgggctggt cgaccgtcaa gggattgtcc accaccgcgg cagagagagt
     5101 ggtctgtgaa cgtcgagctg ggctttttac gtcttatgga gacttcgttg cgcgtactgg
     5161 tctctccgcc gccgttctgt cgcgacttgc ggcggccgat gcgtttcgct cgctggctct
     5221 atcgcggagg ccggccttgt ggatgtcgct ggcgatgacg ccgccggagc cattgctcgc
     5281 cgctcttccc gacgaggctc ctccgcctct gccgcggctt tcaccggctg aggaagtcat
     5341 ccatgattac catgcccagg gattgtcgct ccgcggtcat cccttggctc ctctgcgccc
     5401 gtttctggat aaagatcgcg tcgttccagc gtctgcactc gagagcgtgg aacccgacag
     5461 gcgctatcga gtcgccggct tggttctctt gagacagagg ccgggtactg ccaaaggcgt
     5521 caccttcatg accctcgaag acgagaccgg caacgtcaat ttgatcgtct ggccgcaggt
     5581 atggcagcgg tatcggcgtg ttgcccgcca ggcccgggcg ctcattgcca caggcctctt
     5641 gcagcgccag gacggggtca ttcatgtgat cgtcgatcgc ctcgaagatc tgacgacgcg
     5701 gcttcccgat ctgggacatg tctcgaggga ttttcattga aggattcctc tatctccagt
     5761 gtcgagccgg ctcccgacga tggtaaaatg ccatttgcga ggcgacacat ctgacaactt
     5821 agcgcggcgg ccggcaaagc atttgcgcat ttcgttattt gtcgggaggg attcgagcat
     5881 gcgccagacg ctgaaaactg atcctgccgg aagagaacgc tgtgtcgaag agctggccca
     5941 atcgccggcc ccccagcagc cagctactac agtgagcggc cggccgatcg aaccgctgta
     6001 cactgcagaa ggtctgaacg gactcgacga cga
//
LOCUS       DATAQL010000295         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4303148, whole genome shotgun sequence.
ACCESSION   DATAQL010000295 DATAQL010000000
VERSION     DATAQL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4303148"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1506
                     /locus_tag="VHS97_04980"
     CDS             136..1506
                     /locus_tag="VHS97_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3447582.1"
                     /translation="MSPSKLKTPDTYPRLFPTPDQTIAGIGEALRDGKTTCLAILEGC
                     LVQVDEWEPKVHAWIVLDRDRAIEQARRLDGELKNGNDRGPLHGIPIGIKDIVDVAGL
                     PTACGSKRWAEAVADRDAEVVANLRDAGAVIMGKTVTTPYAWIDPPVTRNPWNLERTP
                     GGSSSGSAAAVACGMCFAAIGTQTGGSITRPASFCGVAGMKPTRLIAAGMMLGIKPFA
                     RSLDHVGPIARSVDDLRSMLGEMIKGPNETKMRLGTDSSLAKPPRLGRLRGFFDRRAE
                     PIVKMAFDEALGTLTSQGAAVVELDDLVDFEQILNDHRRVMAAEAAGVHSNSLDEFPD
                     DYPPRIRELILEGRSLTAVEYLQAKDRKGSVRKSIFTAPACEKLDAFITPATVTKATD
                     PSTTGDPAFNSPWSYSGLPTVSFPTGISPDRLPVAVQLVGYPLKDAELLRIAKWCEQA
                     IRNSRL"
     gene            1520..2914
                     /locus_tag="VHS97_04985"
     CDS             1520..2914
                     /locus_tag="VHS97_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3447583.1"
                     /translation="MADEELKELLDQLDTQTAESDATVERAKESLESTLASLKLTPEE
                     EAVLAGELAQLRELGQKLDDNTIEIAAFGMVSRGKSSVLNALMGQDVFQVGATHGTTV
                     ARAAQRWQHGSPDRPGLEGAKLVVVDTPGIDEVGGEVREALARDVARHADLILFVVSG
                     DMQRREIEALSELREAQKPIILDFNQVDRYPDVDRDQIYAKIKDERVKHLVRPDDIVM
                     TAARPDAYKVKIQLPDGTTQVQWERPAPLIDPLKVRILDVLEREGKALVALNTLLIAG
                     DLHSDIVARKVRIRDDAANRLIWNFSLATGAAVALNPIPVVDIAGGLAVDVGMIVALS
                     NVYGIPLTRRTATSLVRDMMLALGAMGAVGVVSRLVASGIKSSLAGVTILSGGLAAPL
                     TALGYATVGVAQAATAASTSYVLGHGAKTYLRQGCQWGPRGIKTVIQQILTEAKADSV
                     IDRLRDDLKKRLKT"
     gene            2973..3422
                     /locus_tag="VHS97_04990"
     CDS             2973..3422
                     /locus_tag="VHS97_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447584.1"
                     /translation="MKRARVFLIAALMTFLLAAGVAFEVIATQPVRGAMRTCSALFTI
                     ANRPDLTDTERLAAARSLCSTRYLQAHPLALAAEGGLSGFPRNINKNFKAWREGPNVW
                     ICPTNRVGPVYQFVIENGDWRFDGPVAILRPWGEIVRTSELPDSTPQ"
     gene            3573..>6116
                     /locus_tag="VHS97_04995"
     CDS             3573..>6116
                     /locus_tag="VHS97_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3447585.1"
                     /translation="MMSLRPCFIAAILSLFGSGAIGPAYVALGADPAPRTNDAEALSR
                     EEEQFFEKQVRPLLVKHCYQCHSSEAKILKGGLHLDSRGGWMKGGDSGPAIVPSAPDK
                     SLLIEAIRYQSLEMPPKGKLPDEQIVVLEKWVRMGAPDPRTDSGITRPVRPDFDAGRS
                     HWSFRPVVEAPAPRVKNKAWPRSDVDRFILAKLESEGLSPVADADKPTLLRRVFFDLV
                     GIPPTPVEIESFLADDAPDAFVKTVDALLASAEFGQRWGRHWLDVARYADSNGSDENF
                     TYYDAWRFRNYVISAFNADKPFDRFLTEQLAGDLLPFRTQDERDENFVATGFLVVGPK
                     VIGATDKEQLLVDAIDEQVDTIGKAFLGLTIGCARCHDHKFDPISARDYYALAGILAS
                     TETIHGNLLNRRDLTGWNLHPLGPGGQAAYDAFLAHQKTLDGLLKEQKGLQATREGLK
                     KGNKPAAQTKTSEEKPQANLDFKTERERIEAQLAELDRKISDLKKNPVPRPPLAMSVN
                     DHERPGPMAICVRGDAHNRGETVRRGFVSTITPNPPEIGTNVSGRAQLADWLTSSQNP
                     LTSRVAVNRIWQHLFGQALVGTPDDFGTRGERPSNPELLDYLAQRFMRQGWSAKRLIR
                     DLVLSRTYQLSSSFNRAAAARDPENRWLWRMNRRRLQAEALRDGLLAVSGQLDPSPAE
                     SVVAELGAQATGVGVKPRNHVGSVRRTIYLPVIRNDLPALFQLFDFGDSLSVNGRRST
                     TNVAPQALFMLNSPLVFEAAMHTAEIVLEGCDLADEHRLLDGLYVRILGRPPRHDEIE
                     PSLELVHAAMLHSAESETAASKSAAEARSRVRAWAILSQALFCSTKFQYL"
BASE COUNT         1254 a         1881 c         1834 g         1147 t
ORIGIN      
        1 ttcgacctga ccagccgagc cgagtttctc caggatttcg ccaaacaggc cgttcaaaaa
       61 gtgtcggccc ggttgttaca cggtccggcc aaggcgcccg aacagccggc gaccaagaag
      121 tgattgagtt tttacatgtc accttccaaa ctgaaaacac ccgacaccta cccgcggctt
      181 ttcccaacgc ccgatcagac gatcgccggt atcggcgagg ccttgcgtga cggcaagacc
      241 acgtgcctag cgatcctgga aggctgcctg gtacaggtcg atgagtggga acccaaggtc
      301 cacgcctgga tcgtgctcga tcgcgatcgg gcgatcgagc aggcacgcag gctggatggt
      361 gagctgaaga acggcaacga ccgtgggcct cttcatggta ttccgatcgg catcaaagac
      421 attgttgacg tggcgggatt gccgacggcg tgcggctcga agcgatgggc cgaagcagta
      481 gccgaccgcg atgctgaagt tgtcgccaat ctccgcgacg cgggcgcggt aatcatgggc
      541 aagacggtca cgacacccta tgcctggatc gatccaccgg tcacccgcaa cccctggaat
      601 ctcgagcgga cgccgggagg ctcgtcgagc gggtcggccg ccgcggtggc gtgcggtatg
      661 tgcttcgctg cgatcggtac tcagacggga ggctcgatta caaggccggc gtcgttttgt
      721 ggggtggcgg gaatgaagcc gacccgttta atcgccgccg gtatgatgct cggaatcaag
      781 ccgtttgcgc gcagcctcga tcatgtcggt ccaatcgcac gttcagtcga cgatctccga
      841 tccatgttgg gcgagatgat caaaggaccg aacgaaacga aaatgcggct cggtaccgat
      901 tcttcgctcg ctaaaccgcc aaggctgggc cggctgcgcg gtttctttga tcgccgcgcg
      961 gagccgatcg tgaaaatggc gttcgacgaa gccctaggta cgctcacgtc gcagggcgcg
     1021 gcagtggttg agctggatga ccttgtcgac ttcgagcaga tcctcaacga ccatcgccga
     1081 gtgatggccg cagaagctgc cggagtccat tccaactctc tcgacgaatt tcccgatgac
     1141 taccctcctc gtattcgaga actgattctg gaagggcgat cgctgacggc agtcgaatat
     1201 ttgcaagcca aggaccgaaa gggttctgtt aggaagtcga tctttacggc gccagcttgt
     1261 gagaagctcg acgctttcat cacgccagcg acagtgacga aggcgaccga tccgtcgacg
     1321 acgggggatc ctgcattcaa ttcaccctgg agttactccg gcctgccaac ggtctcgttt
     1381 cctaccggta tttcgccgga tcgtttgccc gtcgcggtcc agctcgttgg ttatccactc
     1441 aaggatgcag agctgctccg aattgccaaa tggtgcgaac aagccatccg aaactcacgg
     1501 ctatgaaagg ctacaaccca tggccgatga agagctcaaa gaactgctcg accaactcga
     1561 tacgcaaacc gccgaaagcg acgcgaccgt ggagcgcgcc aaggaaagtc tcgaatccac
     1621 tctggccagc ctcaagctga cccccgagga ggaagccgtg ctggccggcg agctcgccca
     1681 gttgcgcgag ttgggtcaaa agctcgatga taacacgatc gagatcgcgg cttttggcat
     1741 ggtgagccgg ggcaagtcgt cggtgctcaa tgccctcatg ggtcaggacg tctttcaggt
     1801 cggcgccacg cacggaacaa ccgtcgcccg ggccgcccaa cgctggcaac atggatcacc
     1861 cgaccgcccc ggactcgaag gggccaagct cgtggtggtc gatacgccgg gcattgacga
     1921 agtcggcggc gaggtccgcg aagctctggc ccgcgacgtc gcccgccatg ccgatctgat
     1981 cctctttgtc gtctccgggg acatgcagcg cagggagatc gaagctctct ccgagctgcg
     2041 cgaagcccag aaaccgatca tcctcgactt caaccaggtc gatcgctatc ccgacgtcga
     2101 tcgcgaccag atctacgcca aaatcaagga cgagcgggtc aagcatctgg tccgccccga
     2161 cgacatcgtg atgacagcgg cccggcccga cgcctacaag gtcaagatcc agctccccga
     2221 cggtacgacc caggtccagt gggaacgccc cgctcccttg atcgatcctc tcaaagtccg
     2281 gatcctcgat gtcctcgagc gcgagggcaa ggccctggtc gccctcaaca cactgctgat
     2341 cgctggcgac ctgcactcag acatcgtggc ccgcaaggtc cgcatccggg acgacgccgc
     2401 caatcggctc atctggaact tctcactggc aaccggagcc gcggtcgcct tgaacccaat
     2461 cccggtggtc gacattgccg gcggactcgc cgtcgacgtg ggcatgatcg tcgctctctc
     2521 caacgtctat gggatcccgc tcacgcggcg cacggcgaca agcctggtcc gggacatgat
     2581 gctggcttta ggcgcgatgg gggcagtcgg cgtcgtcagc cggctcgtgg ccagcgggat
     2641 caagtcgtcg ctggcgggcg tgacgatctt aagcggcggc ttggccgcgc ctctgaccgc
     2701 cttgggctac gccacagtcg gcgtggccca ggccgcgacc gccgcgtcca cgtcgtatgt
     2761 tttggggcac ggggccaaaa cctaccttcg ccagggctgc cagtggggcc cgcgcggcat
     2821 caagaccgtc atccagcaaa tactcaccga ggccaaggcc gactcggtca tcgaccggct
     2881 acgtgacgat ctcaagaaac gattgaaaac gtgagcgttt tcgaacgacg agataccaac
     2941 gagtcgagcc ggcgcatgcg ggatgcagat cgatgaaacg agcccgcgtc ttcttgatcg
     3001 ccgccttgat gacgtttctg ctcgcggccg gggtggcatt tgaagttatc gccacgcaac
     3061 ctgttcgcgg ggcgatgcgc acttgttccg cgctcttcac gattgctaat cggccggacc
     3121 ttacggacac cgagaggctc gccgcggcgc ggtcactctg ttcgacccgc tatctgcaag
     3181 cccatccctt ggctctcgcc gccgaaggcg ggttgagcgg ctttccacgc aacatcaaca
     3241 aaaacttcaa agcgtggcga gaaggcccaa acgtctggat ctgcccgacg aaccgggtcg
     3301 gcccggtcta ccagttcgtc attgagaacg gcgattggcg ttttgacggc cccgtcgcga
     3361 tcctccgccc gtggggcgaa atcgtgcgga cttcagagct gccagactcg acgccccagt
     3421 aaacgatggc atccgatcaa cagacttgac aaacgagtca tcttggtgaa cgctgaggtc
     3481 tatccgccaa gcctctttga agtcagtcga tcggaaggat tttggcccgc ctgattagtt
     3541 cgactgtcac ctgccggcga tcgagcccga ccatgatgag cttacgacca tgcttcattg
     3601 ccgcgatact ttcgctcttt ggcagtgggg caatcggacc agcttatgtc gcgctcggag
     3661 cagatcctgc gccgcggacg aatgacgcgg aggcgctcag ccgcgaggaa gagcagtttt
     3721 tcgagaagca ggtgcgcccg ctgctcgtaa aacattgtta ccagtgtcac tcatcagagg
     3781 cgaagattct caaggggggt ctgcatctcg acagccgcgg tggatggatg aagggaggcg
     3841 acagtggccc ggcgatcgtc cccagcgcgc cggacaaaag ccttttaatc gaagcgattc
     3901 gctatcaatc gctcgaaatg cctcccaagg ggaagctgcc tgatgagcaa atcgtcgtgc
     3961 tggaaaaatg ggtcaggatg ggagcgcccg atccgcgaac cgattccgga atcacaaggc
     4021 ccgtccggcc cgatttcgat gcgggtcgct cgcattggtc atttcgaccg gtcgtcgagg
     4081 cgcccgcgcc gcgagtgaaa aacaaagcct ggccacgctc cgacgtcgat cgattcattc
     4141 tcgccaagct tgagtcggag ggcctatctc cggtcgccga cgccgacaag ccaacgctgc
     4201 tgcggcgagt cttcttcgac ctggttggca ttccgccgac tccggtcgaa attgagtcgt
     4261 ttctcgcaga cgacgccccc gacgcgtttg tgaaaaccgt cgatgcactt ttggccagcg
     4321 ccgaattcgg tcaacgctgg ggccgtcact ggctcgatgt ggcgcgttat gccgactcca
     4381 acggttcgga cgaaaacttc acctactacg acgcctggcg ctttcgtaat tatgtcatca
     4441 gcgcgtttaa cgccgataag ccattcgacc gtttcctcac cgaacagttg gccggagatc
     4501 tgcttccctt ccgcacccaa gacgagcggg acgaaaactt cgtggcgacc ggattcctgg
     4561 tcgtggggcc caaagtaatc ggcgcgacgg ataaggaaca actgctcgtc gatgcgattg
     4621 acgaacaagt cgacacgatc ggcaaggcct ttctagggct gacgatcggc tgtgcgcggt
     4681 gccacgacca caagttcgat cctatctccg cccgcgacta ctatgctctc gcaggcattc
     4741 tggcgagtac cgagacgatc cacggcaatc tactcaatcg gcgcgatctc acgggctgga
     4801 acctgcatcc gcttgggcca ggcggccaag ccgcctacga cgcgtttctg gcgcaccaga
     4861 agacgctcga cggcctgctt aaagagcaga aagggctcca agcgacgcgg gagggcttaa
     4921 agaaagggaa caaacccgcg gctcaaacga aaaccagcga agaaaagcca caggcgaacc
     4981 ttgatttcaa gactgaacgt gaacggatcg aagcgcaact cgcagagctt gatcgcaaaa
     5041 tctcggattt gaagaagaac cccgttccac gtccaccact ggcgatgtct gtgaacgatc
     5101 atgagcgtcc tggcccgatg gcgatctgcg ttcgcggcga tgctcataac cggggagaga
     5161 cagttcgccg cggattcgtc tcaaccatca caccgaaccc gccggagatt ggcacgaatg
     5221 tcagcggccg cgcgcaactg gccgactggc tcactagctc tcagaatccg cttaccagcc
     5281 gcgtagcggt gaaccgaatc tggcagcact tgttcggaca agcgctggtc ggcacgccgg
     5341 acgacttcgg cacacggggc gaacgtccct cgaatccgga gcttcttgac tacttggctc
     5401 aacggttcat gcggcagggc tggtcggcga aacgattgat ccgcgacctg gtgctcagcc
     5461 gcacgtatca gctgagttcg agttttaatc gcgcggccgc agcgcgagac cccgaaaacc
     5521 gttggctgtg gcggatgaac cgtcgccgac tccaggccga ggcgctgcgc gacggcctgt
     5581 tggccgtcag cggacagctc gacccgtcgc cggctgaatc ggtcgtcgct gaattgggtg
     5641 cccaggccac aggcgtgggc gtcaaaccga gaaatcatgt gggaagcgtc cgccggacga
     5701 tctacctgcc ggtgatccgc aacgatctac ccgcgctctt tcagctcttt gattttggcg
     5761 attctttgtc cgtgaacggc cgtcgcagta ccacgaatgt ggcgccacaa gccctgttca
     5821 tgttgaacag tccgctagtc ttcgaggcgg cgatgcacac ggctgaaatt gttctagaag
     5881 gctgcgattt ggccgatgaa catcgattgc tcgatgggct ctatgtacgc attctggggc
     5941 gtccgccgcg tcacgacgaa atcgagccat cactggagct cgtccatgcg gccatgttgc
     6001 actcggcgga gtcggaaacg gcggcgtcga aatcggcggc cgaggctagg tcgcgcgtcc
     6061 gggcatgggc aattctcagc caggcgctat tttgttcgac gaaattccag tatctc
//
LOCUS       DATAQL010000296         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_29555, whole genome shotgun sequence.
ACCESSION   DATAQL010000296 DATAQL010000000
VERSION     DATAQL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29555"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VHS97_05000"
     CDS             <1..1049
                     /locus_tag="VHS97_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3447586.1"
                     /translation="GYGLGLGLGTGLGWGLSSWMFGPMLYNWGYSNYNNPYYGGYGGN
                     AVVAQQPIVYDYSQPINPQSPAPDETVLSQSMTTFDSAREAFKAGDYARALDLVDQAL
                     KTTPNDAALHEFRALCLFALKKYDEAAAVLYSVLSVGPGWDWTTLIGLYGDPESYTQQ
                     LRALESYCSEHPQSASARFVLAYQYLTQGHADAAVRQLKIVTSLQPKDQLAAQLVQQI
                     QKTDQSAAGTDVAQGGQAAPATTTTPSSTNPPAGVAGKLEGSWTAQPSPDTIITVTFQ
                     DKGRFVWKVSRQGKDQQFEGNSSFENGILTLVQDQNNNAMVGNVNWKDENHFTFKVVG
                     AGPTDPGLSFTKVS"
     gene            1101..1550
                     /locus_tag="VHS97_05005"
     CDS             1101..1550
                     /locus_tag="VHS97_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3447587.1"
                     /translation="MRPLDGDPDIDLSRTVEGGDGIFVIRGGANCRSWNNIQYKVGMS
                     AKNVGSSKLSMNVATIPPGGVAYAHIHVGFELMLYILEGRVRHEYGAGLKNSIDNEAG
                     DFIYIEPGVPHEVFNLSDAEPVVAVVARSDAAEWENIVPYDKNGKDV"
     gene            1590..>4260
                     /locus_tag="VHS97_05010"
     CDS             1590..>4260
                     /locus_tag="VHS97_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2205..2207,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3447588.1"
                     /translation="MNIDSEGTTRAHSRRQFFRGSSALVGAGTLAGLGLNLTPAAAAA
                     QKLKIKGAREVPSVCPYCAVGCGQIVSVRDGQIVNIEGNPDSPISGGTLCPKGAATFQ
                     LAVNPNRLTKVKYRAPYATEWEEKSLDWAMDRIAERVKATRDATFAEYWEGNDEAGKP
                     VKKRVNHCTTIASLGGATMDNEWNYVQLKLWRALGLIWLENQARIUHSSTVPGLGTSF
                     GRGGATTNQIDVQHADAVLIMGSNMAECHPVGFRFVMKAKLNGAAILHVDPRFTRTSA
                     MADVYAPLRAGSDIVFLGGLINHVLEHEAYFREYVVNYTNAAYLIREGYKDSEELDGL
                     FSGFDAQGRKYDPASWGYDNGANEAREAPGPSTAKTGDAESFSARAGHISRPPLSDPT
                     MQHPRCVLQILKRHYARYTPDMVERVCGTPRETFLKVAEALVKASGPDRSAVICYAVG
                     WTQHTTGVQTIRASTILQLLLGNIGRPGGGILALRGHASIQGSTDIPTLYNLLPGYLN
                     TPSALKHHETLADYIAAEMTPTGYWSNFPKFIISLLKSWYGEAATRDNQYGYDWLPKN
                     VGDHSHLPMFIAMAEGKIKGFMAMGQNPAVGGQNAGYQRQALAKLDWMVVRDLYETET
                     ATFWKDSPEVRNGALKPEAIGTEVFLLPAAATAESDGSYTNTHRLIQWHDRAADPPGD
                     ARSDVWFTVHLGFRLKSLYAESRENRDRPIQSLLWDYIDARENEGWKVKDEPSASRIL
                     KEMNGYVVATGAPVKSFSDLKDDGSTACGAWIYSGVFAPTTLEPGGHNHAANRAGDDW
                     VAAGWAFAWPANRRILYNRASADPAGNPWPKEARLARRFGQGSKGYVYWDADAASPDP
                     ARPGQNIRGRWVGLDVPDFPATKPPTAPAQPDKP"
BASE COUNT          898 a         1302 c         1285 g          775 t
ORIGIN      
        1 ttggctacgg tctgggattg ggtcttggta cgggtctggg ctggggactc tcgtcgtgga
       61 tgttcggccc gatgctctat aactggggtt actccaacta caacaacccc tactacggcg
      121 gctatggggg caacgcggtc gttgcacagc agccgatcgt ctacgactat tcgcaaccga
      181 tcaaccccca gagcccggcc ccggacgaaa cagttttgtc gcagtcgatg accaccttcg
      241 acagcgctcg cgaggccttt aaagccggcg attatgcacg ggcgctcgac ctggtcgacc
      301 aggcactgaa gacgacgccg aacgacgcgg ctctgcacga atttcgcgcg ctgtgcctgt
      361 ttgcactgaa aaagtacgac gaggcggcgg ccgtcctgta ttccgtgttg tcggtcgggc
      421 cggggtggga ctggacgacc ttgatcgggt tgtatggcga ccccgaatct tatacgcagc
      481 aactccgggc gctcgaaagt tactgctcgg agcatcccca gtcggcgtcg gcgcggtttg
      541 tgctcgcgta ccagtacctg acccagggac atgccgatgc ggctgtccgg caattgaaga
      601 tcgtgacctc gctccagccc aaagaccagc ttgcggcaca gttggtccag cagatccaaa
      661 aaacggacca aagcgctgcc ggaaccgacg tagcccaggg aggacaggcc gcgcccgcaa
      721 cgacgaccac tccatcgtca accaacccgc ccgccggcgt cgccggaaaa ctcgagggaa
      781 gctggacggc tcagccatcg cccgatacga tcatcacggt gacgtttcag gacaagggac
      841 gtttcgtctg gaaagtcagc cgacagggaa aggaccagca gttcgagggc aactccagtt
      901 tcgaaaacgg tatcctcacc ctcgtgcagg accagaacaa taacgccatg gtcggcaacg
      961 tgaattggaa agatgagaat catttcacgt tcaaggtcgt gggtgccgga cctaccgacc
     1021 caggcctgtc gtttaccaag gtatcgtgag ccgattgttt cagtcggttg cttcttttat
     1081 aaacgggagt cgaaacgacg atgcgtcctc tcgacggcga tcctgacatc gacttgagtc
     1141 gtactgtcga aggaggagac gggatcttcg taattcgcgg gggtgccaac tgccggagct
     1201 ggaacaacat ccagtacaag gtgggaatga gcgccaagaa cgtcggctcg agtaagctgt
     1261 cgatgaacgt ggcgacgatc ccccccggtg gcgtcgccta cgctcatatc cacgtcggct
     1321 tcgagctgat gctctatatc ctggaaggcc gggtacggca cgaatacggg gccggactca
     1381 agaactcaat cgacaacgaa gcgggtgact tcatctacat cgagcccggc gttcctcatg
     1441 aagtgttcaa cctgagcgac gccgagcccg tggtggccgt ggtggctcgt tccgacgcag
     1501 ccgagtggga gaatatcgtt ccttacgaca agaacggcaa ggatgtgtaa gacttccgga
     1561 tccacgaccg tgattggggt ctgatctcga tgaatatcga ctcagaggga accacaaggg
     1621 cgcacagccg gcggcagttt tttcgcggca gcagcgccct agtcggcgcc ggcaccctcg
     1681 cgggattggg cctcaatctg acgcccgcgg ctgcggcagc acaaaagctc aagatcaagg
     1741 gcgcgcgtga ggttccgagc gtctgcccgt attgcgctgt cggctgcggc cagattgtca
     1801 gcgtgcgcga tggtcagatc gtcaatatcg agggcaatcc cgacagcccg atcagcggcg
     1861 gcacgctctg ccccaagggc gccgcgacct ttcagcttgc cgtcaatccc aatcgcctca
     1921 cgaaggtcaa atatcgcgcg ccctacgcga ccgagtggga agaaaagagc ctcgactggg
     1981 cgatggaccg gatcgccgag cgcgtaaaag ccacgcgcga cgcgaccttt gccgaatatt
     2041 gggaaggcaa cgatgaggca ggcaagcctg tcaagaagcg cgtcaatcat tgcaccacga
     2101 tcgcgtcgct ggggggcgcg acgatggaca atgaatggaa ctatgtgcaa ttaaagcttt
     2161 ggcgagcact gggattaatc tggctcgaaa accaggcccg gatatgacac agctcgaccg
     2221 tgcccggtct gggcacttcg tttggtcgcg gcggtgcgac caccaatcaa atcgacgtgc
     2281 agcacgccga cgctgtcttg atcatgggct ccaacatggc ggagtgccac cctgtcggct
     2341 ttcggtttgt gatgaaggcc aagctaaatg gggcggcgat tctccacgtt gatccgcgct
     2401 tcacccgcac ctcggcgatg gccgacgtct acgctccctt gcgcgcgggc agcgatatcg
     2461 tctttctggg gggcctgatc aaccacgtgc tcgagcacga agcttatttc cgagaatatg
     2521 tcgtcaacta caccaatgcg gcatacttga ttcgcgaagg ctataaagac tccgaggagc
     2581 ttgatggcct attttcgggt tttgatgccc aaggacgcaa gtacgacccg gcaagctggg
     2641 ggtacgacaa cggtgcaaac gaggcccgcg aagcccccgg tccgtcgacg gccaaaactg
     2701 gagatgccga gtctttttcg gcgcgggcag ggcacatttc caggccgccg ctctctgatc
     2761 cgacaatgca acacccccgg tgcgtgctcc agatcctcaa gcggcattac gcgcgttata
     2821 cacccgacat ggtcgaacgg gtgtgcggaa cgccccgcga aaccttcttg aaggtcgccg
     2881 aggcgctggt gaaagcctcc ggaccggacc gctccgcagt gatttgctac gcagtgggct
     2941 ggacgcaaca caccaccggc gtgcagacaa ttcgcgcttc cacgatcctt caactcttgc
     3001 tcggcaacat cggccggccc ggcggtggca tccttgcact gcgcggtcat gcgtcgattc
     3061 agggttccac ggacataccg accctgtaca acctcttgcc gggttatctc aatactccga
     3121 gtgctctgaa acatcacgag actcttgccg actacatcgc cgccgagatg acgccaaccg
     3181 gctattggtc caactttccc aagttcatca tcagcttgct caagtcttgg tatggagaag
     3241 ccgcgacccg cgacaatcag tacggttacg actggctgcc caagaatgtg ggcgatcatt
     3301 ctcacttgcc gatgtttatt gcgatggccg aaggcaagat caagggcttt atggcgatgg
     3361 gccagaaccc cgccgtcggc ggccagaacg ccggctacca gcgtcaggcg ctagccaagc
     3421 tcgactggat ggtggtgcgc gacctctacg agaccgagac ggccactttc tggaaagaca
     3481 gtccggaggt tcggaacggg gccttgaagc cagaagcaat tggtaccgag gtcttccttc
     3541 tgccagcggc cgccaccgcc gagagtgacg gcagctacac caacacgcac cgcctgatcc
     3601 agtggcacga ccgtgccgcc gatcctcctg gcgacgcccg gtccgacgtc tggttcacgg
     3661 tgcacctcgg gttccggctc aaatcgctct atgcagagag ccgcgagaac cgggatcgtc
     3721 ccatccagtc gctcttgtgg gactatatcg acgcgcgtga aaatgaaggc tggaaggtca
     3781 aggacgagcc ctcggcctcc cgaatactga aggagatgaa cggctacgtc gtcgccactg
     3841 gagcgccggt caagagcttc agcgacttga aagacgatgg ttccacagct tgcggcgcgt
     3901 ggatctatag cggtgtcttt gcgccgacga cgctggaacc cgggggacac aaccacgcgg
     3961 cgaatcgagc cggcgacgac tgggtcgccg cgggctgggc gttcgcctgg ccagccaacc
     4021 gaaggattct ctacaaccgc gcatcggccg accccgccgg aaatccatgg cccaaagagg
     4081 ccaggctggc ccggcgcttc gggcaggggt ccaaaggata cgtctactgg gatgccgatg
     4141 ccgcgagtcc cgacccggcg cgtccgggtc agaatattcg cggccgctgg gtcggcctcg
     4201 acgtaccgga ctttccggcc accaaacccc cgaccgcgcc cgcccagccg gacaaacccg
//
LOCUS       DATAQL010000297         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4457216, whole genome shotgun sequence.
ACCESSION   DATAQL010000297 DATAQL010000000
VERSION     DATAQL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4457216"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHS97_05015"
     CDS             <1..202
                     /locus_tag="VHS97_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447589.1"
                     /translation="VVDVLQTTLVVRCKFTATPSRPTYLQRQALRHLIDAFAASQIRF
                     AAPNVTLQLASAGAGWPLKLCE"
     gene            353..1153
                     /locus_tag="VHS97_05020"
     CDS             353..1153
                     /locus_tag="VHS97_05020"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3447590.1"
                     /translation="MIDRLLDELRQRTDEVAELNRGLEARVAEQVEELGRVGRLKRFL
                     APQLAELIVSQGDEKILESHRREIVVVLCDLRGYTAFTETAEPEEVLDFLREYHGALG
                     PLVSQFEATLDQFSGDGIMVFFNDPVPCPDPAERAVKMAMSMREEAGKLIATWRRDGC
                     ELGFGTGIAQGYATLGQIGFSERSGYTAIGTVCNLAARLCAEAKDGQILITSRVARAV
                     AAVARLEDLGNLELRGLRRPVAAFNVVQGTSAADARPNLTVVAREPGA"
     gene            complement(1438..1581)
                     /locus_tag="VHS97_05025"
     CDS             complement(1438..1581)
                     /locus_tag="VHS97_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447591.1"
                     /translation="MARQQDRKLAELASLRDDVNVLTAIIQRLDTKVIRLLAEIRATH
                     SQV"
     gene            complement(1628..1816)
                     /locus_tag="VHS97_05030"
     CDS             complement(1628..1816)
                     /locus_tag="VHS97_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447592.1"
                     /translation="MSQDGRFEIKWKTTPVPTGASEPPASADLSRGEPASTGEPVPPP
                     ASRGRIDADETMRALMRN"
BASE COUNT          335 a          629 c          624 g          355 t
ORIGIN      
        1 ggtcgtcgac gtattgcaga cgacgctcgt cgtccgttgc aaattcaccg cgacgccgtc
       61 gcgtccgacc tatttgcagc gtcaggcgct gcgtcacctg atcgacgcct ttgccgcatc
      121 ccagatccgc tttgcggcgc caaacgtcac gctgcagctg gcgagcgccg gcgctggctg
      181 gccgctaaag ctgtgcgaat gaccgggtgc cggctcgggg accatcgcgg cgacgcggac
      241 ccgtcccaag caacccaacg gcacaatggc cgcgctaacc gaatgcacgg tgccgcgtac
      301 cgcaagttcg aaatcgagtc tagagcgaac ccgacatccc gacgggctgc aggtgatcga
      361 ccgtttgcta gacgaattgc gccaacgcac cgacgaggtg gccgaactga accgtggtct
      421 cgaagcgcgg gtcgccgaac aggtcgagga actggggcgc gtcggtcggt tgaagcggtt
      481 tcttgccccg caactggccg aactcattgt ctcacagggc gacgaaaaga tcctggaaag
      541 ccaccgacgg gagatcgtcg tcgtgttgtg cgatcttcgc ggctacaccg cgttcaccga
      601 aaccgccgag ccggaggagg tcctcgattt cctgcgggag taccacggcg cgctgggacc
      661 gcttgttagc cagttcgagg cgacgctcga ccaattctcg ggcgatggga ttatggtgtt
      721 ttttaacgac ccggtaccat gtcccgaccc ggcggagcgt gcggtgaaga tggcaatgtc
      781 catgcgagaa gaggcgggca agctgatcgc gacttggcga cgcgatgggt gtgagcttgg
      841 tttcggtacg ggtatcgcac agggttacgc tacattgggt cagatcggct tttccgaacg
      901 ctccggctat accgccatcg gaacggtgtg caaccttgcc gcgcggttgt gtgccgaggc
      961 gaaggacggg caaatcctga tcaccagccg ggtcgctcgc gcggtggcag cggtagcgag
     1021 gttggaggat ctcggaaacc tggagctgag gggtcttcgc cgtccggtcg cggcattcaa
     1081 tgtcgtgcag ggcaccagcg cggcagacgc tcgtcctaat ttgaccgtcg tcgcgaggga
     1141 gccgggggcc tgatcttaga caacgcgagg tctcaaagcc acccaaccga gccaattccc
     1201 gcgccgaccg cgaaggtctc ttaaggggca ctttgaccca ttccgccgcc taggctgaat
     1261 ggctgctgtc cgttcagtta gccgacctct gccggactgc gccgcaatga ggaagatgcg
     1321 ccgaaagcgg ccctcaccac acgcgacacg cggcagcgaa ccggcggggt tacccgaagc
     1381 tgccgttcgt ggccgcgaga cacggccgca cggccgcacc cgtgactcaa tccgcaccta
     1441 cacttggcta tgggtcgcgc ggatctcggc cagcagccgg atgaccttgg tgtcgagccg
     1501 ctggatgatt gccgtcagca cgttcacgtc atcgcggagg ctcgcgagct ccgccagctt
     1561 gcgatcctgc tggcgtgcca aaaaactcca acgtgaccgg ttcgcgttcg ctcatttcgg
     1621 ctcgatctca gttgcgcatc agcgcgcgca tcgtctcatc ggcatcgatc cgcccacgcg
     1681 acgccggcgg cggcaccggc tcgccggtcg atgccggttc gccccggctc agatcggcac
     1741 tcgccggcgg ctcgcttgcg ccggtcggga ctggcgtggt tttccacttg atctcgaaac
     1801 gcccgtcctg actcactgca ccgctccttg ttcggtcgcg gtggcgccgg cctgctgcgc
     1861 cttgtgcgcg gccacaagcc gatcgcggta ttgccggacc atcttcgcgt agctattgac
     1921 gccgttgcac ccgtacccgg cca
//
LOCUS       DATAQL010000298        10983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_791712, whole genome shotgun sequence.
ACCESSION   DATAQL010000298 DATAQL010000000
VERSION     DATAQL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791712"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /gene="xseB"
                     /locus_tag="VHS97_05035"
     CDS             complement(<1..345)
                     /gene="xseB"
                     /locus_tag="VHS97_05035"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX3447593.1"
                     /translation="MPDESEPELSFEQALGQLERIVASLERGEPELTSALAKYETGVR
                     LLKTCYGLLDQAEQSVALLTGVDPAGNPLSAPFDASATIITREVVSAVTLSPEVESNP
                     PKKRKPTATPPSP"
     gene            complement(520..1683)
                     /gene="xseA"
                     /locus_tag="VHS97_05040"
     CDS             complement(520..1683)
                     /gene="xseA"
                     /locus_tag="VHS97_05040"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX3447594.1"
                     /translation="MSSIPWRQRHEAEDPRGPTFESVSELTERIKRSLETDFGQVALH
                     GEVSNVARPRSGHIYFTIKDELASIRAVMWKNDSRRVAFDLTDGLAVRALGRLTVYAP
                     RGEYQIVVSAIEPEGMGALELAFRQRYARLALEGLFDTERKRPLPRFPRRIVIVTSPT
                     GAAVRDILQVLSRRWRCASVLVAPTRVQGVGADMEVVAALDLANHVAQADVIILARGG
                     GSLEDLWTFNEEIVVRAIVTSRLPVVSAIGHEIDVTLADLAADRRALTPSEAGEVCVP
                     DAREVTLHLDRLADRLRLAGSSQLRDARARLDQLAKSSLQAIQQDLVGRRQQLRRLGA
                     SLDALSPLAVLARGYSLTFLADGKTLVRSSEDVKSGELIRTHLAAGQISSHVV"
     gene            complement(1782..2768)
                     /locus_tag="VHS97_05045"
     CDS             complement(1782..2768)
                     /locus_tag="VHS97_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447595.1"
                     /translation="MRRLITLATGGMRRITSWKFVFYELLLPMLRWLGPARGDGLLGL
                     LGWVSVLARPRRGTRLREALLRARAALDADWPIETTWSTLAANTARFLARDYPLDRQP
                     DEAVLKRFDVRGFERLFTTLNEGNGAILVGSHLGAHIAGLHWLFRRGLPLRLLVQRPR
                     HVSRELNHRFDAGGIHPQVEMFLRRDLSPAVAIERVFRARAALRDGMAIYLNGDIPWS
                     GPNTCAGRLLGRPQRLLAIWTELAVLTGAPVFLVFCTHLPRGRFALEIEAMGHLRPGE
                     EQTAVADYLKQLEARIATSPADAVAHLVWPCYQSPPAPHKHHRNRKEVGSRR"
     gene            complement(2765..4396)
                     /locus_tag="VHS97_05050"
     CDS             complement(2765..4396)
                     /locus_tag="VHS97_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3447596.1"
                     /translation="MGGIHLSELLEDAAARRPDHPAVEDEHGRSLTFAELLQSADRLA
                     TRLMRWGVGRGDRVGLWLPKSVEAVTAVHGILRTGASYVPVDPTGPPGRAAGILAASG
                     VKAVVVAAELMPALHEAYPGDGPRPRLIVVDEDSGARDGHGPESNDPLPSSVISGDAR
                     WSEVIADDAPSPLMPPRAAGDLAYVLFTSGSTGQPKGVMLSHLNAFTFLGWCQRTLGP
                     WNDDDRFASHAPFHFDLSVFDLFAACQGASTLVLIGENLGKDPVALGEFLTERRISIW
                     YSAPSILALLAERGKLERPGHNAPRLVLFAGEVFPIVSLARLRKLWPRAAMWNLYGPT
                     ETNVCTAYPIPAWIPLDRTEPYPIGTVCTPLCARVVDELGYDVEPGSLGELVISGPGV
                     MRGYFGQPELTAAVFLVDDDGSRWYRTGDMVTDGGDGCFQFHGRRDRMVKKRGYRIEL
                     GEIESALYRHDGVDRAGVVAQADDRGVSIAAFVALKPDQKRSIIAMKRHCTHHLPHYM
                     IPDTITFLDTMPATSTDKLDYQRLRTLASQQGPRA"
     gene            complement(4451..5767)
                     /locus_tag="VHS97_05055"
     CDS             complement(4451..5767)
                     /locus_tag="VHS97_05055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3447597.1"
                     /translation="MTAPEMASVATARLGIVKSLALLVLGLFPWPLDWSKLRDVVDSA
                     RSPELNRADRDGHAAGYYEGIIGGGHGSDTARDDASQRLIGKPSGWIPFQDADVVHYL
                     DGEFLQFELKPLVQRVLFGKTFSTNAFGMHDDPLSKEKPAGTLRIAVLGSSMDMGWGV
                     KHEDTYINQLEAWLNLHAVRQQRKEPRRFEVLNFAVAAYSPMQRLETLRTKVMAFQPD
                     LVIYSATTLDTRLMEIHLCDMLKKRIDLKYDFLRQAMTLAAVNDDDLRIDAGGEMINK
                     SRLKQKLRPLYWGIYDRTLGAIAGECRVAGIPVVMVIIPRVGKADSPSARAEPVARLK
                     AIAAHQGLTVFDLSDAFDAFDPAQLEIAAWDDHPNALGHHRLFLSLARAMKNTDIYDL
                     LFPSGHDQEASNAASLSPEQTALDTKPGAGKHKESLIAKKGSVGER"
     gene            complement(5764..6990)
                     /locus_tag="VHS97_05060"
     CDS             complement(5764..6990)
                     /locus_tag="VHS97_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3447598.1"
                     /translation="MSRQVIATLMILACGLVPARLSGDGASRLLDSLKSNMLNRIDLE
                     RIEQGYYDKLLDTSRRLDDLADLPDLRIGRRQGRTWSVPFDDAPLVMRVDDMREVVLK
                     ADDAAERRGVLWHTNSLGMRDQAYALEKPPRTFRIALVGDSIGAGWGVDVEQRFESIM
                     EEMWDARARGASGWEVEIINCAVPGHSPGQRWCHFTQVGWPMKPDLVIYESTAADVGW
                     DERRLRYLLARGLAWDSPMYHGVLERAGLERLGSPDDYKRALRPRHWEILAGVYQTMA
                     AECRERGVPIIWVVVPRVGRPNDMAVHKALYETARAAGFSQIVDVTDAYDDVDPARLA
                     LDPDDFHPNAKGHDRLARRLDLAMRSLPELSRLWSGDQAHSNVDESATSHERSTEARA
                     RAAAEVPLEEREGEPQ"
     gene            complement(6998..8422)
                     /locus_tag="VHS97_05065"
     CDS             complement(6998..8422)
                     /locus_tag="VHS97_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447599.1"
                     /translation="MRLQTRSSVEPRPKRALHQQYFPWPHRDLDPGLLLDSGLYEGEP
                     ETCRRSWRPFVRNFLYLALLLAVFKVYRIEERAFQGRAFQMLVTLALSALPVHYMAPF
                     RWKKPLFVAISILGLFCVCGAQVAAIVLGLATVLIGICFLPIPWTLRAALMGALALAL
                     ALARSGLSRNVIPDNVLPIIASMFMFRMLIYLYELKHVKTRETLVDTLGYFFLLPNYC
                     FMHFPVVDYRTMQRGYFAVDIHDLQRRGLQMMLRGTVHLLFYRLVYHEMLIPAGRVHD
                     LASLCGYLVCNYLLYLQVSGQFHIACGMLQLFGFQLPETHHHYLLATGFTDYWRRINI
                     YWKDFMVRVFFNPVVFRLKRWPQPLALAIATISVFLATWILHAYQSYWLRGSWGFSVP
                     DALFWGILGGLVMVNVQLDARRSRAKGRAAGRGGARPTLRLLAVRSLKVAATFTTIAV
                     LWSLWSSPSLSEWLNMLNRGFHGS"
     gene            9367..10197
                     /locus_tag="VHS97_05070"
     CDS             9367..10197
                     /locus_tag="VHS97_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447600.1"
                     /translation="MASRFRGELGVIVGWLVFPLVPVILEDIYYQICTNPFSTARFGP
                     APHDWGWGVWVVMLGPLVGFGFLAGATVDLPDDLATPRRGWRRLLARRSIWVAAGPWC
                     GFIALGLLFLAMGILVKLMPSLPIPDLPQWWRGSWFETVLQWVFTVLFVVTIAYAWLW
                     PAWAALRRAASAGRFKRALYRGLTVALAFVGSLFGSFWAATSWWRSHFFDSRVPLLIA
                     AITLMMTSGCSHTLTYGELRRRELFHALLVAWVLGLALMWRWWSRRRPGPPPTGTTPS
                     "
     gene            10386..10952
                     /locus_tag="VHS97_05075"
     CDS             10386..10952
                     /locus_tag="VHS97_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447601.1"
                     /translation="MPVLLRRAFERAFVKGIGVAVAIVISLGPQVPLARGQSPATPTG
                     VTLDQLLAMSTAELDTVYRQGASVAIPEGRIRGTAILSPGTRRTRALSRGARLMWQGK
                     VFEPGQTSAVNRFFGMRLVRAQVYQGPSWLDGQPSLILDYSQTSRVYADNRDEIRQVA
                     PGLFLGLMYDRTTSPPGLVMYFALESRP"
BASE COUNT         2116 a         3398 c         3340 g         2129 t
ORIGIN      
        1 cggggagggc ggagtcgccg tcggtttccg tttcttggga ggatttgact caacctctgg
       61 actgagtgtc acagcggaga cgacttcccg cgtgatgatg gtggcgctcg catcgaatgg
      121 agcggaaagc ggattgcccg cagggtcgac cccggtcaag agtgccacgg attgctccgc
      181 ctgatcgagg aggccgtagc acgttttgag caacctcacc cctgtttcgt acttcgccag
      241 cgcggaggtc agctcgggct cgccccgctc taaacttgcc acgatccgtt cgagctgacc
      301 gagcgcctgc tcgaagctca gctcaggttc tgattcatca ggcatgggca agtctctgga
      361 ctgaccagtc gcatcgcaaa cctgcgagca gggccggtta ttccggacga tgactcccgc
      421 agagtttcgg gtccctttca cgaaactaac gtccgcgacc gatcttcgcc agcagcgagc
      481 aacgaaaaca ggtccgtccc agcatgatgc tgttttggct cagacgacat gactgctgat
      541 ctgtcccgct gcaaggtgcg tgcggatcag ttcgccgctc tttacgtctt cgcttgagcg
      601 gaccaaggtt ttgccgtcgg ccagaaaggt caggctatag ccgcgcgcaa gcacggccaa
      661 ggggctaagc gcatcaaggc tggcgccaag acgcctcaac tgttgccggc ggccaaccag
      721 atcttgttgt attgcttgca agctactctt ggctagttga tcgagacggg cacgcgcatc
      781 gcgaagttgg ctcgatccag cgaggcgcaa ccggtcagca aggcggtcga ggtgcagagt
      841 gacctcgcgc gcatcgggca cgcaaacttc gccagcctcg ctgggggtca gggcgcgtct
      901 gtcggccgcg aggtcggcga gggttacatc gatttcgtgg ccgatcgccg acaccacggg
      961 aagacgagac gtcacgatgg cacgaaccac gatttcctcg ttaaaggtcc agagatcctc
     1021 caagcttccg ccgcctcgcg cgagaatgat cacatcggct tgcgcgacgt ggttggccag
     1081 atccaaggcg gcaacgacct ccatgtcggc accgactccc tggactcggg tcggagcgac
     1141 caggaccgag gcgcaccgcc agcgccgcga aagcacttgc aagatatcgc ggactgccgc
     1201 tccggtcggg ctggtgacga tcacaattcg ccttggaaac cgtggcaacg gccgctttcg
     1261 ctctgtgtcg aacagacctt cgagcgccag ccgtgcataa cgctggcgaa atgccaactc
     1321 gagagccccc attccctccg gctcgatcgc gctcaccacg atctggtatt cgccgcgcgg
     1381 tgcgtatacc gtcagccgtc ccagtgcccg cacggccagc ccgtcggtca gatcgaaggc
     1441 gacacgccga ctgtcgtttt tccacatgac cgcgcgaatc gaggccaatt cgtctttgat
     1501 ggtgaagtag atgtggcccg accgcggccg tgcgacgtta gaaacctcgc catggagagc
     1561 cacttggccg aaatcggtct cgagacttcg cttgatccgc tcggtaagct cggacactga
     1621 ttcaaaggtt ggcccgcggg gatcttcggc ttcgtgcctt tggcgccacg gaatgctgga
     1681 catgagcggt gtcatccaga gtgtttgcgt ccggccaaga ttcagtacgg ggatcttccc
     1741 gtaatcctga gtcccgagca agatacaatt tcgagcaccg cctaccgcct actgcctact
     1801 tccttccggt tccgatggtg tttatggggc gctggcggcg attggtagca gggccagacc
     1861 aagtgggcga cggcgtcggc aggagacgtg gcgatccgcg cttcgagctg tttcaagtaa
     1921 tcggcgacgg cggtttgttc ctcgcctggc cgcaggtggc ccatggcttc gatctcaagt
     1981 gcgaaacggc cacggggcag gtgggtgcag aataccaaaa agaccggcgc gccggtgagc
     2041 acggccagct cggtccagat ggccaagagg cgctgggggc ggcccagcag tcgtccggcg
     2101 cacgtatttg gtccactcca tggaatgtcg ccgttcaagt agatcgccat tccgtcgcga
     2161 agcgctgccc gcgcccggaa cacgcgctcg atggcgaccg ccggcgaaag gtcacggcgc
     2221 aagaacatct cgacctgagg atggatgccc cccgcatcga atcggtgatt gagctcgcgg
     2281 gaaacgtgcc gcggccgctg caccagcaaa cgaagcggga gtccgcgacg aaacaaccag
     2341 tgaaggccgg cgatatgcgc tcccagatgg ctgccgacca agatcgcgcc gttaccttcg
     2401 ttaagcgtgg tgaagaggcg ctcgaagccg cgcacgtcga agcgcttcaa gacggcttcg
     2461 tcgggctggc gatcgagtgg atagtcgcgc gccagaaaac gcgcggtgtt ggccgccaaa
     2521 gttgaccagg tcgtctcgat cggccagtcc gcgtcgaggg ctgcgcgggc acgcaacagc
     2581 gcctcgcgga gacgcgtgcc gcggcgcggc ctcgctaaca ccgacaccca ccccaagagc
     2641 cccaacaatc catcgccacg cgctggccct agccagcgca gcattggcaa tagaagctcg
     2701 taaaacacga acttccagct cgtgatacgg cgcattccgc cggttgccag cgtgatgagt
     2761 cgcctcacgc gcgtggtcct tgttgggagg ccaatgttct cagacgctga tagtcgagtt
     2821 tgtcggtcga ggtggctggc atggtgtcga gaaacgtgat cgtatcgggg atcatgtagt
     2881 ggggcaggtg gtgcgtgcag tgccgtttca tggcgatgat cgaccgtttc tgatcgggct
     2941 tgagcgcgac gaacgcggca atcgagacgc ccctgtcgtc ggcctgggcc acgaccccgg
     3001 cacggtcgac gccgtcatgg cgataaaggg ccgactcgat ctcgcccagc tcaattcgat
     3061 agccgcgttt cttgaccatc cggtcgcggc ggccgtgaaa ctggaagcaa ccgtcgccgc
     3121 cgtcggttac catatcgccc gtgcgatacc atcggctgcc gtcgtcgtcg accaagaata
     3181 ccgcggcggt cagctcaggc tggccgaagt agccccgcat cactccgggg cctgagatca
     3241 ccagttcgcc gagtgatccg ggctcaacat catagcccag ttcgtcgacg acgcgcgcac
     3301 agagcggcgt acagaccgtc ccgatcgggt acggctcggt tcgatcgagc gggatccagg
     3361 ccggaatcgg atatgcagtg cacacgttcg tctcggtcgg cccatacagg ttccacatcg
     3421 ctgcccgagg ccatagcttc cgcaaccttg caagactgac gatgggaaac acttcgccgg
     3481 caaataacac cagacggggc gcgttatggc ctggccgttc cagcttcccg cgctcggcca
     3541 agagcgcgag aatcgaggga gcagaatacc atatgctgat ccgtcgctcg gtcaggaact
     3601 cgccaagagc gacggggtcc ttgcccaggt tttcaccgat caacaccaag gtcgacgccc
     3661 cctgacaggc cgcaaagagg tcgaacaccg acaggtcgaa atggaacgga gcgtgcgatg
     3721 caaaccggtc gtcgtcattc catgggccca gcgttcgctg gcaccacccc aggaaggtga
     3781 acgcgtttaa gtgcgacagc ataacgccct tgggctgccc cgttgatccc gacgtgaaga
     3841 gtacgtaggc cagatcgcct gcagcgcgcg gcggcatgag cggcgaaggc gcgtcatcgg
     3901 caatgacttc ggaccagcgg gcgtctccgg agatgaccga gctcggcaac gggtcgttgg
     3961 attctggacc atgcccatct cttgcaccag agtcttcgtc gaccacgatc aggcgaggac
     4021 gcggtccgtc acccggatag gcctcgtgca gagccggcat gagctccgcg gccacaacga
     4081 ccgccttgac accactggcc gcgaggatgc cggcggctcg tccggggggg ccagtcgggt
     4141 ctacaggaac gtacgacgcg cccgtgcgta agatcccgtg aaccgccgtc acggcctcaa
     4201 cacttttggg caaccagaga cccacccggt cgccacggcc gacgccccat cgcatcaacc
     4261 gcgtcgccag ccgatccgcg ctctgcaaca actcggcgaa ggtcagggat cggccgtgct
     4321 cgtcttccac ggccggatgg tcgggccgcc gcgccgcggc atcctcgagc aattcgctta
     4381 aatggattcc gcccatgtgt tcatgcccgt ttagcagttt ttctgcaagt ctgtcctcga
     4441 tcaacaatca tcatctttcg cctacagaac ctttcttggc gatcagagat tctttgtgct
     4501 tgcccgctcc aggcttggtg tcgagcgcgg tttgttctgg actcaaagag gccgcgttac
     4561 tggcttcttg gtcgtggccg gacgggaaaa gcagatcata aatgtctgtg tttttcatgg
     4621 cgcgagccag ggacaggaac aagcggtgat gacccagcgc gttggggtgg tcgtcccagg
     4681 cggcgatctc gagctgcgcc gggtcgaacg cgtcgaacgc gtccgagagg tcaaagaccg
     4741 tcagtccctg gtgcgccgcg atggccttga gtcgggcgac gggctcggcg cgggctgacg
     4801 gtgagtcggc cttcccgacc cgtggaatga tcaccatcac cacggggatg cccgcgaccc
     4861 ggcactcgcc ggcaatcgca cccagagttc tatcgtaaat cccccagtag agcggccgca
     4921 gcttttgctt gagccggctc ttgttgatca tctctccacc ggcgtcgatg cgcaaatcgt
     4981 cgtcgttcac cgcggccagg gtcatcgcct ggcggaggaa gtcgtacttc aggtcgatac
     5041 gcttcttgag catgtcgcag aggtggattt ccatgagtcg ggtatcgaga gtggtcgccg
     5101 agtaaatcac caggtctggc tgaaatgcca tcaccttggt gcggagcgtc tccaaacgct
     5161 gcatagggct ataggcggcg acggcaaaat tcagcacttc gaaccgccgc ggctccttac
     5221 gctgttggcg gacagcgtga agattgagcc acgcttcgag ctgattgata taagtatcct
     5281 catgctttac gccccagccc atgtccatgg acgagcccag tacggcgatg cgcagagttc
     5341 ccgcgggctt ttccttcgat agcgggtcgt cgtgcatgcc aaaggcgttg gtcgaaaacg
     5401 tcttgccaaa aaggacccgc tgcaccagcg gcttgagctc gaattggaga aattcgccgt
     5461 cgagataatg aacgacatcg gcatcctgga aggggatcca gccgctgggc ttgccgatca
     5521 accgctgcga tgcatcgtct cgcgccgtgt cggagccatg tcccccgccg attatgccct
     5581 cgtagtagcc tgccgcgtgc ccatcgcggt cggcccggtt cagctcgggc gagcgagcgc
     5641 tgtcgaccac gtcgcggagc ttgctccaat ccaggggcca gggaaagagg cccaagacca
     5701 agagagccag cgatttcacg atgccgaggc gggccgtcgc gaccgatgcc atctccggtg
     5761 cggtcattgt ggttctcctt ctcgctcttc gaggggaacc tcggccgcgg ctcgcgcgcg
     5821 ggcttcggtg ctgcgctcat gactcgtagc ggattcgtcg acgttcgagt gagcctggtc
     5881 cccggaccaa agccgactca gctcgggcag acttctcatg gctaaatcga gccgacgcgc
     5941 cagcctgtca tggcccttgg cgttgggatg aaagtcgtcg ggatcgagcg ccaatcgcgc
     6001 cggatcgacg tcgtcataag cgtcggttac atcgacgatc tgggaaaatc cagcggctcg
     6061 tgccgtctcg tagagcgcct tgtgaacggc catatcgttc ggccggccga ccctgggaac
     6121 gaccacccag ataatcggca cgccccgttc gcggcactcc gcggccatcg tctgataaac
     6181 gcccgccagg atctcccagt gccgcggccg taaggctcgc ttgtaatcgt cggggctgcc
     6241 cagccgctcg agaccggctc tctcgagaac gccgtgatac attggcgaat cccaggccag
     6301 cccgcgcgcc agcaggtagc gcagccgccg ttcatcccag ccgacgtccg ccgcggtcga
     6361 ttcgtagatc acaaggtccg gcttcatggg ccagccgacc tgggtgaaat gacaccagcg
     6421 ctgtcccgga gaatggcccg gaaccgcgca gttgatgatc tccacttccc aaccgctcgc
     6481 tcccctggcg cgagcatccc acatttcttc catgattgac tcaaacctct gctcgacatc
     6541 aacgccccag ccggcgccga tcgagtcgcc cacgagcgcg atgcgaaaag ttcgcggcgg
     6601 tttttcgagg gcgtatgcct ggtcgcgcat gcccagcgag ttggtgtgcc acaacacgcc
     6661 tcgacgctct gcggcgtcat cggctttcaa aaccacttcg cgcatgtcat caacccgcat
     6721 gaccagtggc gcgtcgtcga acggcaccga ccaagtgcga ccctgccgcc gcccgatgcg
     6781 caggtccggc aggtcggcca ggtcatcgag ccgccggctc gtgtcgagca acttgtcgta
     6841 gtagccctgc tcgatccgct cgagatcgat tcggttgagc atattgctct tcaagctgtc
     6901 caagagtcgc gacgcaccat cgccggagag ccgcgccggc acgagcccgc aagccaggat
     6961 catgagtgtg gcgatcacct gccggctcat tatcgcctca tgacccgtga aaaccgcgat
     7021 tgagcatatt gagccactcc gacaggctcg ggctcgacca cagcgaccag agcacggcga
     7081 tcgtcgtgaa tgtcgccgcg accttgagcg agcgcacggc caaaagccgc aaggtgggcc
     7141 gggccccgcc ccggccggcg gcgcgtccct ttgcacggct gcggcgtgcg tcaagctgca
     7201 cattgaccat caccaatccg cccaggatcc cccagaagag agcgtcggga accgaaaaac
     7261 cccagcttcc ccgcagccag tacgactggt aggcatgcaa gatccacgtg gccagaaaga
     7321 ccgaaatagt ggcgatcgcc agcgccaagg gctgcggcca ccgcttgagc cggaacacga
     7381 ccggattgaa gaaaaccctc accatgaagt ctttccagta gatgttgatc cgccgccagt
     7441 agtcggtaaa acccgttgcc agcaggtagt ggtggtgtgt ctcgggaagc tggaagccaa
     7501 agagctggag catgccgcag gcaatgtgaa actgccccga aacctgaaga tataagagat
     7561 aattgcagac caagtatccg cagagactgg ccaggtcgtg cacccgtcca gcgggaatca
     7621 acatctcgtg atagacgagt cgataaaaga gcagatgcac ggttccccgc aacatcattt
     7681 gcaacccgcg ccgctgcagg tcgtggatgt ctacggcgaa atagccgcgc tgcatcgtgc
     7741 ggtaatcgac caccgggaag tgcatgaagc aatagttcgg cagcagaaaa aagtaaccga
     7801 gagtgtcgac caaggtttct cgtgtcttca catgtttaag ctcatacaaa taaattaaca
     7861 tgcgaaacat gaacatcgag gcaataatag gcaagacatt gtcgggaatc acgtttcgag
     7921 atagcccgga tcgcgccagg gccagtgcca gtgctagggc ccccatgagc gcggccctaa
     7981 gggtccatgg gatgggaagg aagcagattc cgatcaaaac cgtcgccagt ccgagcacga
     8041 tcgccgcgac ctgcgcgcca cagacgcaga acaagcccag aatcgagatc gccacgaata
     8101 agggcttttt ccagcggaag ggagccatgt aatgaaccgg cagcgcggac agggcgagtg
     8161 ttaccagcat ctggaacgcg cggccttgaa acgcccgttc ctcgatccgg taaaccttga
     8221 ataccgccaa gaggagtgcc aggtaaagga agttgcgcac gaacggccgc cagctccggc
     8281 ggcacgtttc cggctcgcct tcatacagtc ccgagtccag taagagccca gggtcaaggt
     8341 ctctgtgcgg ccaagggaag tactgctggt gcagcgcccg tttcggccgc ggttcgaccg
     8401 agctcctcgt ctgcagcctc atgggtccgc ctccccctcc cggctcgtct tccacggtcg
     8461 tcgcgctatt tcgacaaggt ccggccgctt ggctcgcgcc tcactctgcg gaagggcggc
     8521 acttgcttcc tgatccattg agggacagga tcagccgtcc ttttcttcca ctaaaactca
     8581 cggcaacgtt cgggtctcga agtttcgagc gatcaggatg gcaggacgcc agacgtggga
     8641 tgctcgacgt tgctctgcgt tcaagtgaga tcgcgggggc cggttcctcc gtgaaccggt
     8701 aagttccccg ctgcggccgc gccatccgtg atacgctaca ttttcggtca ctgtcactgc
     8761 gttgttgttt gcattgtcat cgctagaagg ggagcactcc tgtgcggagc atcgacgagg
     8821 actctgctgg gaagcacgag gttcgctcgc gcgaactcgc acataccaat caaagaccga
     8881 cctcttcagt agcctcccct tagacaaacg cgatataacc aatctttctt ggacctgtca
     8941 acccgactgc ctccgtcgac tcgagcgggt cacaacggac cctgcaagca gacttgatgt
     9001 tctggtattt ttcgcgtgtg aatttttcct gaattcgaag gaattcgcca tgccttgatc
     9061 gacctcgcgt tttcaaatcc ggttcatcga gcccattgtt cttgcgaggc aggtctgggg
     9121 atcggctcag caaaaagaga gcggccgccc cgtcccccgc tctgggcggc ttacgcagac
     9181 tccccggtcg agtatctagc gactgaactg tgcggatgat tgaaccacct caaattcatc
     9241 atcggcttcg tggtattatg accgttggaa accggcaggg agattgtcag gtgccctttg
     9301 gcccgctcaa tgttccggcg cagagatcgc gaccggcttg gtcgtgactt gtagagggag
     9361 ccagtcgtgg cgagtcgctt tcgcggtgag ctcggcgtga tcgtgggctg gctggtgttt
     9421 ccgctggttc ccgtgatttt ggaagacatt tactaccaga tatgtacgaa cccgttttca
     9481 acggccaggt tcgggcccgc tcctcatgat tggggctggg gagtatgggt cgtgatgctg
     9541 ggcccgctcg tcggctttgg cttcctcgcc ggcgcgaccg tcgatttgcc tgacgacctc
     9601 gctacgccac gccgaggctg gcggcggtta ctcgcgcggc ggtcgatctg ggtggcggcc
     9661 ggcccttggt gtggttttat cgccctgggt cttctgtttc tcgccatggg aattctggtc
     9721 aaactcatgc cgtctctgcc aatccctgat ctgccacaat ggtggcgcgg ttcgtggttc
     9781 gagacggttc tccagtgggt tttcacggtg ctgtttgttg taaccatcgc ctatgcctgg
     9841 ctctggcctg cgtgggccgc gcttcggcgg gcggcatcag ccggtcgatt caaacgcgcg
     9901 ctttatcgtg gcttgaccgt ggcgctcgca ttcgtcggat cgctcttcgg tagtttctgg
     9961 gcggccacgt cgtggtggcg aagccatttc ttcgattccc gagtacccct gttgatcgcc
    10021 gcgatcacct tgatgatgac tagcggttgc agccatacgc tcacttacgg cgagctgcgg
    10081 cgccgtgagt tatttcacgc gctgctggtg gcctgggttc tcggattggc tttgatgtgg
    10141 cggtggtgga gtcggcgccg gccagggccg ccgccgactg gaacgacacc ttcatgatca
    10201 ggccccattg ccttccgccc tgggcggctc gcttggaacg cgcgagattt cggcgaccaa
    10261 aatagtgatc gaacgtcatc ggtccggccg atctatagag acacggtttg cggtgcgcgc
    10321 ttgaagtaac aatcgcgccg aattgacgac gaagaggctt acaaccagat gcggaggcta
    10381 ccgctatgcc cgtactgctc agacgtgcat tcgaaagagc atttgtcaaa ggaataggcg
    10441 ttgccgtcgc gatcgtgatt tctcttggcc cccaggttcc tctggcacgc ggtcagtcac
    10501 ctgctacacc gaccggcgtt actctcgacc agttgctcgc aatgagcacc gccgagttgg
    10561 acactgttta tcgtcagggc gcctcggtgg cgattcccga agggcgcatc cgaggaacag
    10621 caatcttatc gcccggaacc agacgaacac gtgccttatc acggggcgcg cggctgatgt
    10681 ggcagggaaa ggtgttcgag ccaggtcaga ccagcgcggt caaccgcttt ttcggcatgc
    10741 gcctggtccg ggcccaggtc tatcaaggac ctagctggct tgatggccag ccgtctttga
    10801 ttctcgacta cagccagact tcgcgcgtct acgccgacaa ccgcgatgaa attcgccagg
    10861 tcgcacccgg cctctttctc ggtcttatgt acgaccgtac gacatcaccg ccagggcttg
    10921 tgatgtattt cgcgctcgaa tcgagacctt gaaaagtcgg caaatgacgc cgccactctt
    10981 tcg
//
LOCUS       DATAQL010000299        11307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4915051, whole genome shotgun sequence.
ACCESSION   DATAQL010000299 DATAQL010000000
VERSION     DATAQL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4915051"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VHS97_05080"
     CDS             <1..337
                     /locus_tag="VHS97_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447602.1"
                     /translation="STSAATSELGVHAGLTSIDPSLEHHSHVEFNPRAMRKYRGVRGR
                     KVSSSGKRAWFNFTIFAGEKSWVAGVELATVSEPPARMPRIWGRRPSGVDPSHPLRCN
                     QLLNLAGKR"
     gene            complement(408..1217)
                     /locus_tag="VHS97_05085"
     CDS             complement(408..1217)
                     /locus_tag="VHS97_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447603.1"
                     /translation="MNVVRCSRTWFVVASVLALVCYIASFADDSESPHQLSLADLASY
                     RAALSGRATGENTKTSDAPVRVIFRDLWNRPDAFRGRRVIIQGRVERIFRQGPIGSFP
                     ALAEVWIVSPAGDPFCAVVPNESGTGILPVADHGLEGHATSKQSPELGQTVRLTGTFL
                     KMVRYAGGDAARLAPLVVGDQPPVPAPEITQANNLGGGIHSDGGQRSTAGSPTGWLLG
                     LAITMLIAGVLALQYLRAQSRRAGLWDSRRRTTALLEADPPLEFIEQGDDR"
     gene            complement(1214..1867)
                     /locus_tag="VHS97_05090"
     CDS             complement(1214..1867)
                     /locus_tag="VHS97_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3447604.1"
                     /translation="MSTGAGLGMGLSLLDAKASPALAVANPASPRARRYRPGPPEEVA
                     FPVAPMLDMAFQLLAFFILTFKAPSAETHLDLNLPATPVALPAASRGEAHPTPARNVD
                     SDLENDLLVRAEADDLGDLKALRLGEAVLPDLATLGKRLHRYTKLLDGRPLRVRLVAD
                     DGLRYEPAARIIATCSAAGVAAIRLTQPGATPPIPGGGGDPRGLNKSRSPAPSGAVR"
     gene            complement(1867..2343)
                     /locus_tag="VHS97_05095"
     CDS             complement(1867..2343)
                     /locus_tag="VHS97_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3447605.1"
                     /translation="MPRRSTTAPSSILTPNLMPLLDVVLQLITFFMMLVHFGTRLEGA
                     TKAVRLPVAPAALPGSDLTFDRLGVVMNAQGQLLVSGRALDDEAASEWWADQAKTRRA
                     GLESLGGPAAANDNLPTVVILRADRAASYGSVRRALAEAQDKGFAHFSLVVLRRSR"
     gene            complement(2358..3314)
                     /locus_tag="VHS97_05100"
     CDS             complement(2358..3314)
                     /locus_tag="VHS97_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3447606.1"
                     /translation="MFAVVCGALLWSPHGLTATRAVAQDRGSTRAPAPASANPATINT
                     EDEADAGVDSRGATRGESSDSLLTSIAHASTPVIVVTLVIATMSFYLIALVVWMGLHF
                     RSTVAVPGDLVRDIQIALEQKKYNEAYQRLAEDPSFLARVLASGVRKLPAGLSHAQRA
                     MELANENQTMEMEHRTTYLATVGTLGPMIGLVGTVYGMIMAFHVIAQEGATPQASQLA
                     AGISTALYATLEGIAISIPAIFFYAMFRNRIARLSLEVGITAEPLLEQFVPGVRPVMP
                     GPTVNHGSAQSPPAHPHPFAVSAALAATGGNAPRPALPATEH"
     gene            complement(3524..4810)
                     /locus_tag="VHS97_05105"
     CDS             complement(3524..4810)
                     /locus_tag="VHS97_05105"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX3447607.1"
                     /translation="MTQTQTKCRVLEAILRANHGRKPKLLRLKLRRMLADPFTFFRGT
                     NHLFVADWPELQPPEVGPDILICGDLHLENFGAYRTADGDFRYDINDFDEAMIASSSL
                     DLVRCTASIFLAAEQWKLLPTHATSMALAYLEHYREAILAATRVGAVGEVAPHSGHGA
                     IWELLGSTASGIQEALLEKNTKRRSSAPPKIRRTDSHIAISPKRFELIRGAVEAHGQK
                     LGMADQFRVHDVTGRIAGVGSLGVRRYLALLEGEGPPDGHRLLDIKQVEPSALTGCTT
                     AAQPDYGGNEAKRVVQAQTMLQGHPSAGLDALDIGPHACCIREMIPEENRSSLDRLRR
                     QPAKLRQAVETAGLLTAWSHLRGGRPTPDQKQDHWPELADWSGSAAIDAVLAAAARYA
                     ERTNQNYAEFRQSVRDAGGVGECLQSTRETEKVLSV"
     gene            complement(5112..5420)
                     /locus_tag="VHS97_05110"
     CDS             complement(5112..5420)
                     /locus_tag="VHS97_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447608.1"
                     /translation="MIMRQAKTSLRKPPRRDQGTSDHDPAQLSASLRELALRAFQLVR
                     ARDESTSESEHGADCSEQELKDVHGRIVQLQRALRAHHMDDLAAYVTALRERVEERLA
                     "
     gene            complement(5658..7283)
                     /locus_tag="VHS97_05115"
     CDS             complement(5658..7283)
                     /locus_tag="VHS97_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX3447609.1"
                     /translation="MARWFVDRLPQRGIPSVSLRRLLPEAQFVGCADWNVSGCTDDHR
                     RLEPGQVFVAAREALPGYDGHLFVHEALERGAAGVVLERPCAEAGRLQVVVPDAMAAH
                     ARICHALAGDPSHQLATLGVTGSFGKTITALMVHSIIEAAGDRCGLAGSLGFHDGVND
                     RPVGAAFETRAGGMRMGTGSTSDLNRRDHEPGAYAPGARGVAALLVAMVERRCKGAVL
                     EVSSAALARRSFDGIAFHAAVVTDIAAPVGFPPEVLLQKRRAKTRLFRQIAPGGVAVV
                     NADDPHAEVLGGINIDARRVAFAMEPTTAARNVVDVSAKLERIDGSGTRMVLHGFDRE
                     AAVHLPLIGPRVAVCALAAAALAWAVEIDLADVVAGLEAVQCVAGHLESVALGQDFDV
                     RIDAAQSPDDVAGALTALRALSAGRVHVVVSAEGGGDRADRLRLAEVVERGADRAILT
                     LSNPRTEDPNQILDDLLAGFRRPGKIRIEPNRQTAIETALADARRGDIVLITGKGRHT
                     YQIFADSVIPFDDHAVVRGWLQAHKATLSQRSA"
     gene            complement(7479..9203)
                     /locus_tag="VHS97_05120"
     CDS             complement(7479..9203)
                     /locus_tag="VHS97_05120"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX3447610.1"
                     /translation="MTSNDANGVAPANRPSVRHDQGTGRPHNPAEIAAQTDLHRIDTP
                     AEWKRIERVWWVALSVAMLVFSAFPVANYFMGLSTKDYGLWYQVGLAVRHGLEIYPRP
                     ESGRLFPFMYPPSAAAMLTWVSMLGPIGSLAALLLVNSVAWVVSIRLSLWLAVGPGVR
                     RHPLVVILPSLSIIVLVYNIYLLGQPNLLLLALFLGSFACLRLGRHIGAGALLATAAA
                     IKAFPILAIGYLIYRRMWTATAATVAMLAAWLLIAPLPFRTPAQAVDDLVVWSQGMVF
                     TYNSHGIAQRPFRSYSFKNQSIMALAHRLLRDVPADGESVLSRRVRAGSLGPRPSKGL
                     LTVDPSTDLPSFLKSTVRPGANAARPGGASDEASLDAASAKPTEAAPRWDDVLQESES
                     ALRNAWRVNVTHLNFRVVTVITVAAMLVLCIFVAAVMPSRNRQTPQTAAIEFALVTLL
                     TVMFSPLSFNYAYVWLIYPITLGLQLVMSEAPTAPWHKLKIAWIATVILIPALAIPMP
                     LVAQAYGNLFVPALLLVIGLGLILATANCHRSLTSVVVPDAHTGIAFFSALANNAASS
                     GEASSAKV"
     gene            complement(9330..>11307)
                     /locus_tag="VHS97_05125"
     CDS             complement(9330..>11307)
                     /locus_tag="VHS97_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447611.1"
                     /translation="MSQHYQTNPERPRARNPFVSPELEALILALLAKRPDERPGSGAL
                     AGLALRQEVARIRERDGSGGSAATASVGVGAAGTTNLTGTVGLTAPVVTEPLPAQSIQ
                     PANGLTATRTSSALTASSPSNTISGETLVTGAGPPAPTAATIPSSPSKHPQEGGPSSP
                     APVAIATVGGTGGVTALIRSPLARRMLEVVLAEPILLSPDERYLHGHYLAYLMSGSRR
                     RGLFLRRPLEPRNADRGRLLLGVTYAILAGGGEDAIRDAATLLNQKIEVRPILSPIVV
                     AKYLTCRESPAKRKLFRQTRKAIAQASVYAQKHMIDSKGVLNPGLMPQKLEDLNLIAP
                     ARDAVDDVLVERWNRVAEVWRNEVAFRAAVLRYATSNAHRDPASAALWPEVVYPLIER
                     ARWHRRIRTRTEAVWDYLSGRLHVADAGVVLDRALNRSVPAPLVAELDDELSLFGDNP
                     RLDDEADPFAAAAPTDDDRLTATLTNSRVNLAELAVEEKEARGPLALADPNPIRFSQG
                     ELHELWKEAFAVLQSQAQARPGAKPSSHRHVPIGPYRLVVIPSIRGRAAGQIAIQGMT
                     NKQIELSTPTVRTKGSSAKPLVAVWVYSDNSLAIVHLDFMNSERYILWHAPRGHQLNF
                     DDASDLNHELYTLGLEIPDQLERVLARSFKPRAQ"
BASE COUNT         2187 a         3437 c         3600 g         2083 t
ORIGIN      
        1 gtcaacaagc gccgccacca gtgaactagg tgttcacgcg ggcttgacgt ctatcgaccc
       61 ctcgctcgag caccactcgc atgtagaatt caacccccgg gcaatgcgga agtatcgcgg
      121 cgtccgtggg aggaaggtgt catcgtcagg aaaacgagcg tggttcaact ttaccatctt
      181 tgcaggtgaa aagagctggg tggctggggt cgagctggcg actgtcagcg agcccccggc
      241 gcggatgcct cggatctggg ggcgtcgccc gtcgggcgtc gatcccagcc accccctgag
      301 gtgtaaccag cttttgaacc ttgccggaaa acggtgatac gcttgacctc aagaccggcc
      361 gttttcagga aatcgaggcg atcgaagttt gaatcgcggc ggattcttca gcgatcgtca
      421 ccttgctcaa tgaattccag aggaggatcg gcctccagga gagcggtcgt cctgcggcgc
      481 gagtcccaaa ggcccgctcg ccgcgactgt gcacgcaaat actgcagggc aagcactccg
      541 gctatgagca tcgttatggc cagaccaagc agccagcccg tcggtgaccc agccgtcgac
      601 ctttgtccgc cgtcggaatg aatgcccccg ccgaggttat tggcctgggt gatttccggt
      661 gctggcaccg gcggctggtc tcccacaacc aagggtgcca aacgcgctgc gtcccccccc
      721 gcgtagcgga ccatcttgag aaacgtaccc gtgagccgga cggtttgacc cagctctggg
      781 ctctgctttg atgtggcatg gccttccagg ccgtggtcag ccacgggcaa gatgcccgtg
      841 ccactctcgt tcggaacaac cgcgcagaag gggtcaccgg cgggcgagac aatccagacc
      901 tctgccaggg cggggaagct gccaatcggc ccctggcgga agatccgttc gacacggccc
      961 tggatgataa cccggcggcc gcgaaatgcg tcgggtcgat tccagaggtc cctgaaaatc
     1021 acccgcaccg gcgcgtcgga cgtcttggta ttctcgcccg tcgccctgcc cgagagggcc
     1081 gcgcggtatg acgccaggtc ggccaggctg agctggtgcg gggattcgga atcatcggca
     1141 aaagaggcga tgtagcagac gagcgccagt acggacgcta ctacgaacca tgttcgggaa
     1201 caacgcacga cgttcatcgc actgctccag atggggcagg ggaacggctc ttgttcagac
     1261 cgcgagggtc tcccccgcca ccgggaatcg gaggcgtggc tcctggctgg gtcagccgaa
     1321 ttgccgcgac tccggcggcc gagcacgtag cgatgatccg ggctgccggc tcatagcgca
     1381 gaccatcgtc ggcgaccagg cggacgcgga gcggccggcc atccagtagc ttggtgtagc
     1441 ggtgaagacg cttgccaagt gtggccagat ccggcagcac ggcttcaccc agccgcagtg
     1501 ctttgaggtc gcccaggtca tcagcctcgg cgcggaccag caggtcgttt tccaggtcgc
     1561 tatcgacatt ccgcgcgggc gttggatggg cttcgcctcg agacgcagcc ggcagcgcca
     1621 ccggcgtcgc cggcaggttt agatcgagat gggtctcggc ggaaggagcc ttgaaggtca
     1681 agatgaagaa cgccagcaat tgaaacgcca tgtcgagcat gggcgctacc gggaatgcaa
     1741 cctcttcagg cggtcccggc cgatagcggc gcgcccgggg cgaggccgga ttggccactg
     1801 ccaatgccgg tgatgccttt gcgtctaaca ggctcagccc cattcctagc ccggctcccg
     1861 tgctcattag cggctcctcc gcaagaccac caggctaaag tgcgcaaagc ctttgtcttg
     1921 tgcctcggcc agcgcccgtc tgacggaccc atacgaggca gcccgatccg cgcgcagaat
     1981 cacgacggtc ggcaaattgt cgttggccgc cgctggcccg cccagacttt ccagcccggc
     2041 gcggcgggtt ttagcctggt cggcccacca ctcagacgcc gcctcgtcat cgagcgcacg
     2101 gccactgacc agcagctggc cctgggcgtt catcaccacg ccaagtcgat caaatgtcag
     2161 gtccgacccg ggtaacgccg cgggagctac cggtagccgt actgctttgg tcgcaccctc
     2221 gagtctcgtg ccgaaatgaa ccaacatcat gaaaaacgtg atgagctgaa gcaccacgtc
     2281 caggagcggc atcagattgg gagtgaggat gctcgaaggc gcggttgtgc tgcgacgggg
     2341 catgtgtgcc ttctttctca atgttccgtc gctggcaggg ccgggcgcgg ggcattgcct
     2401 ccggttgccg ccagggcggc actgacggcg aagggatgag ggtgtgccgg aggcgattgg
     2461 gccgacccgt ggttgactgt cgggccaggc atcaccggac gcacgccagg cacgaactgc
     2521 tctagcaacg gctcggccgt gatacctact tcaagagata atcgcgcgat tcgattacga
     2581 aacatggcgt aaaagaagat cgctggaatc gagattgcaa ttccttcgag ggttgcgtac
     2641 agagccgtcg agataccagc cgcgagctga ctggcctggg gagttgcgcc ttcctgggcg
     2701 atgacatgga atgccatgat catgccgtaa accgtgccga ccagcccgat catcggtcca
     2761 agtgtcccca cggtagccag ataagtcgtg cgatgttcca tctccatggt ttggttttcg
     2821 ttggccagct ccatggctcg ctgggcatgc gagagcccag ccggcaattt gcgcaccccg
     2881 ctggccagca cccgtgccag gaacgacgga tcttccgcca gccgttgata cgcctcgtta
     2941 tactttttct gttccagcgc gatttgtatg tcgcgcacca gatcgccagg taccgcgacg
     3001 gtcgagcgaa agtgcaggcc catccacacc accagggcga tcaagtaaaa cgacatcgtg
     3061 gcgatcacga gagtcacaac gatcaccggc gtcgaggcat gggcgatcga cgtcagaagt
     3121 gagtcggatg actcgccacg agtcgcgcct ctgctgtcga cgcccgcgtc cgcttcatcc
     3181 tcggtgttaa tcgtcgcggg attcgcactc gccggagctg gtgcacgcgt cgatccccga
     3241 tcctgggcaa ccgcccgcgt cgcggtcagg ccatgtgggc tccacagaag ggcgccacag
     3301 accacggcga acatgagcgc accccgaacg ggtcggttcc caacaggaca ctcaagccaa
     3361 tgaccgcacg atttcaacat ggaacgcaaa cctcctgctt gcgttcagct tatcgcatcg
     3421 cgacggtacg gacaacgcga cgtgcgtcca ggcttttcgc caggtcaggc ccgcttgatg
     3481 cactgtgcgg cgagggcgag ttcggcgatt cggagaaacc caatcacaca ctcaagacct
     3541 tttcggtctc gcgagtggac tggaggcatt cgcccacgcc gccagcgtca cggacggatt
     3601 ggcgaaactc agcataattt tggtttgtgc gctcggcgta gcgggcggcg gcggcaagca
     3661 ccgcatcgat agcggcgctg cctgaccagt cagccagttc gggccagtgg tcttgctttt
     3721 ggtccggggt ggggcggccg ccacggaggt gcgaccatgc cgtcagcagg ccggcggttt
     3781 cgactgcctg gcggagcttg gccggctgcc tgcggagccg gtcgaggctc gaacggtttt
     3841 cctcgggtat catttcccga atgcaacatg cgtgtggacc aatgtccaat gcgtcgagtc
     3901 ccgcggacgg atggccctgc agcatagtct gcgcctgcac gacccgcttt gcctcgtttc
     3961 caccataatc gggttgcgcg gcggtggtgc accccgtaag tgccgacggc tcgacctgtt
     4021 tgatgtcgag aagccggtgg ccgtcaggcg ggccttctcc ctcgagcaag gccaggtacc
     4081 gacggacacc caagctgccc acgccagcta ttctgccagt gacgtcatgg actcgaaact
     4141 ggtcggccat gcccaatttc tggccgtggg cttcgacggc accacggatc agttcgaacc
     4201 gcttgggcga gatcgcgatg tgactgtccg tgcggcggat tttgggcggg gcactacttc
     4261 tgcgcttcgt gtttttctcg agaagggctt cttggatgcc actggccgtc gaccccagga
     4321 gttcccagat cgccccatgg ccactgtggg gagcaacttc accgaccgcc cccactctgg
     4381 tcgccgccag gatggcttcg cggtagtgtt ccaggtaggc caaggccatg ctcgtggcgt
     4441 gcgtcggcag gagcttccat tgctccgcag ccaaaaaaat actggcggtg caccggacca
     4501 ggtccaggct actggatgcg atcatcgcct cgtcgaagtc gttgatgtcg tagcggaagt
     4561 ccccgtccgc cgtccgataa gcgccaaaat tctcaagatg gagatcacca caaatcaaga
     4621 tatccggccc gacctcaggg ggctgtagtt cgggccaatc ggcgacgaag aggtgattgg
     4681 ttccccgaaa gaatgtgaac ggatccgcga gcattcgcct cagcttgagc cgcaacagct
     4741 taggctttcg tccatggttc gcgcgaagga tcgcctcaag gacccggcac ttcgtttgag
     4801 tctgggtcat ggtcggtctc ttttcgttct caatggaaaa tcggctatga gccgcgaccc
     4861 gcgtctctgg ccatcgttac cgtcgtcggt agcgaccaca atacgtgttg acgagaaatt
     4921 gtcgcttttg cgaagccggc gcggcgaacg cgttctatag gcggagggcg gattagaggt
     4981 cgtcgttgtt gacgtgatca ccagccggaa ttcaggcgga cacgacgatc tcttgaagcg
     5041 atttggtggg ccaggggatt tttgtgaagg gattcgctcg cgggatgact cgaactgtcg
     5101 aaaatcccgt ttcaggcgag gcgctcctcg acgcgctccc gcagggccgt gacgtaagcg
     5161 gccaagtcat ccatatgatg cgcgcgaagc gctcgttgga gctgaacgat ccgtccgtgc
     5221 acgtctttca gctcttgttc cgagcaatcc gctccgtgct cggattcgga cgttgattcg
     5281 tccctcgcgc gtacgagctg gaatgctcgt aacgccagtt cgcggagcga ggcactgagc
     5341 tgggcggggt cgtggtcact ggttccctgg tcgcgccggg gaggctttcg gagggatgtt
     5401 tttgcttgtc tcataatcat ctgcggtcct cgcgggttat taagtcctat ttgactctag
     5461 gtcagtggcg tccattgtga ttggtaattc gttaccctga ggtgattctt caactcactg
     5521 cccaggggaa tgccagcgcg acgcttccga tctactgatc agacgcatcc gcgtggactg
     5581 ggttcgcgat atctatcgat cagcgtgccc caaggcgcaa atcctcatgg aatcatgtct
     5641 aaggatatta gctcagctca ggcggagcgc tgagacagcg tagctttgtg ggcctggagc
     5701 cagccgcgaa cgacggcgtg gtcgtcgaag gggataacag agtctgcgaa gatctggtaa
     5761 gtgtggcgcc ccttgccggt aatcaacacg atgtcgccgc ggcgggcgtc ggccagtgcg
     5821 gtttcgatgg cggtctgtcg gttgggctcg atgcgaatct tgccggggcg tctgaagccg
     5881 gccagcagat catcgaggat ctggttgggg tcttcggttc gggggttgct gagcgtcagg
     5941 attgcgcgat cggcgccgcg ttccacgacc tcagcgagcc gaagtcgatc ggccctatcg
     6001 ccgcccccct cggcgctcac gacaacatgg acgcgtcccg cggacaaggc ccgcagcgct
     6061 gtcaaggccc cagccacatc atcgggagat tgagcggcat cgatccggac gtcgaaatcc
     6121 tgacctaggg cgaccgattc caggtggcca gcgacgcact ggactgcctc aagcccggcg
     6181 acgacgtcgg ccagatcgat ctctacggcc cacgccagcg cggccgcagc gagcgcgcag
     6241 acagctaccc gcgggccgat caggggtaag tgcaccgctg cctcacggtc aaatccgtgg
     6301 agaaccatcc gagtgcccga gccatcgatc cgctccagct tggcactcac gtcgacgaca
     6361 tttctggcgg cggttgtcgg ttccatagcg aaggcaacgc ggcgcgcatc aatgttgatc
     6421 ccacccagga cttctgcatg ggggtcgtca gcgttgacca cggcaacgcc tcccggcgcg
     6481 atctggcgaa acagcctggt cttggcccgg cgcttctgga gcagcacttc gggcgggaac
     6541 ccaactggag cggcgatgtc ggtaaccacc gcagcgtgaa aggcgatgcc gtcaaagcta
     6601 cggcgggcca gagccgcgct cgaaacttcg aggacagccc ccttgcaacg gcgctcgacc
     6661 atcgccacaa gcagtgcggc aactccgcgt gcgcccggcg cataggcccc aggctcatgg
     6721 tcgcgccgat tgagatccga ggttgacccg gtacccatgc gcatcccacc ggcccgtgtc
     6781 tcaaacgccg ctccgaccgg gcggtcattg accccatcgt gaaagcccag cgagccagca
     6841 agtccacatc gatctcctgc ggcttcgatg atcgagtgca ccatcaatgc cgtgattgtt
     6901 ttcccgaagc tacccgtgac accgagcgtc gcgagttggt gcgaggggtc gccggccaac
     6961 gcgtggcaaa tccgcgcgtg cgcggccatc gcgtcgggaa cgacaacctg gagtcgaccg
     7021 gcttcggcgc acggtctttc gagcaccact ccggccgccc ctcgttcgag cgcctcgtgc
     7081 acgaagagat gcccgtcgta cccgggcagc gcctcgcggg ccgcgacgaa cacctggccc
     7141 ggctccagcc ggcgatggtc gtcggtgcag ccagagacat tccagtctgc gcatcccaca
     7201 aattgggctt cgggcagcag tcgcctcagg ctgaccgagg gaatcccacg ttggggcaga
     7261 cggtcaacga accagcgcgc catcggcatc cgcctccttg cttcgctttt gtcccgctcg
     7321 cggctgtttc tggcggggat cggaccaaac acaaacccgc gtcttacctc gtccgtcggg
     7381 gagagcagac gttggtacgt gagttccccc ccttgagacg ttcacttagc cgcagcctga
     7441 atcgcgatca acccgcgacc gcgatccgtc catccgcgtc aaaccttcgc gctggatgcc
     7501 tcgcctgaag aggcggcatt gttggccaaa gccgagaaaa aggcaatgcc agtgtgagcg
     7561 tccgggacaa ccaccgaagt aagggatcga tggcaattgg ccgtggcgag tatcagcccc
     7621 aacccaatga ccaagagcaa cgcaggcacg aacaggttgc cgtaggcctg tgcaacgagc
     7681 ggcatgggga tcgccagcgc agggatcaaa ataaccgtcg cgatccaagc aattttgagc
     7741 ttgtgccacg gcgcggtcgg agcttcgctc attaccaatt gcaggcccaa ggttatcgga
     7801 tagatcagcc agacgtaggc gtagttgaat gacagcgggg aaaacatcac ggtaagcaag
     7861 gtgaccaacg caaactcaat ggcagcggtc tgcggtgtct ggcgatttcg cgatggcatc
     7921 actgccgcga caaatatgca taaaaccagc atggcggcga ctgtgatcac cgtcaccaca
     7981 cggaaattca gatgggtcac gttaaccctc caggcattgc ggagcgcgga ttcagattcc
     8041 tgaaggacat catcccatcg aggggctgcc tcggtgggct tcgcgctcgc agcgtcgaga
     8101 cttgcttcat cgctcgcgcc gccgggtcga gccgcatttg cacctggccg gacggtggac
     8161 ttcaagaatg aaggcaagtc ggtggacggg tcaacggtga gcagtccctt ggacggccgc
     8221 gggccgagac tgccggcccg tactcgccgc gacaaaaccg actcaccgtc agccggcaca
     8281 tcgcgaagca gccgatgcgc caaggccatg atcgactggt tcttgaagct gtaggatcga
     8341 aaaggccgct gggcgatacc gtgagagttg taggtaaaaa ccatcccctg tgaccagacg
     8401 accagatcgt cgaccgcctg ggctggtgtg cgaaaaggca ggggggcaat cagtagccac
     8461 gcggccagca tggccactgt cgctgccgtg gcggtccaca tccgccggta aatcaaatag
     8521 ccaatcgcca ggatcggaaa cgccttgatg gctgcggccg tggccaatag cgcaccggcc
     8581 ccgatatgcc gtccgagccg aaggcaagca aacgaaccca agaaaagtgc caggagcagc
     8641 aggttgggct gacccagcag gtatatgttg tagaccaaaa caatgatcga gagcgacggc
     8701 aggatgacaa ccagcggatg tcgtcgcact ccggggccga ccgccagcca aagggacaac
     8761 cttatcgaga cgacccacgc gaccgaattc accagcaaga gggccgccag cgacccgatc
     8821 ggtcctaaca tactgaccca ggtcagcatc gccgcggcgg atgggggata cataaacggg
     8881 aagaggcggc ccgattccgg ccgcgggtag atctcaagcc catgccgtac ggccaaaccc
     8941 acttgatacc agagaccgta gtctttggtt gaaagcccca tgaagtagtt ggcgaccgga
     9001 aaggctgaga aaacgagcat cgccaccgag agggcgaccc accacacgcg ctcgattcgt
     9061 ttccattcgg cgggggtgtc aattcgatgg agatcggtct gggcggcgat ttcggctgga
     9121 ttgtgaggcc ggcccgtgcc ttgatcgtgc cggacggatg ggcggttcgc gggtgccact
     9181 ccattggcat cattcgacgt catgtcagtc gggtctccct gtttaacaat ccgtccacgc
     9241 gaatcctaac cgaaagcgag gaaaccacca aggtgaaagc gacatcgccg aaatcaagcg
     9301 tgcaatgagc tcacgagcgt gggtcatgac tattgggcac gaggcttaaa gctacgtgcg
     9361 agaacgcgtt cgagttgatc ggggatttcc agaccgagcg tgtaaagctc gtggttcaag
     9421 tcggatgcgt cgtcgaagtt gagctgatgg cctctgggtg cgtgccagag gatatagcgt
     9481 tcggaattca taaagtcgag atgaacgatg gcgaggctgt tatcgctata gacccagacg
     9541 gccacgagtg gtttcgcgga ggagcctttg gtgcggacgg tgggggtgga gagttcgatt
     9601 tgcttgttcg tcattccctg gatggcgatc tgacccgcgg cccggccgcg tatggacgga
     9661 atgacgacta gcctgtacgg gccgatcggt acgtggcgat ggctgcttgg tttggcgccc
     9721 ggccgggcct gggcctggct ctggagcact gcgaaggctt ctttccagag ctcgtggagc
     9781 tcgccttgag agaaacggat cgggttggga tcagccagtg ccaatgggcc tcttgcctct
     9841 ttctcttcaa ccgccagttc cgccaggttg acacggctat tggtgagcgt ggccgtcagt
     9901 cggtcgtcgt cggtaggggc ggctgccgcg aacgggtcgg cttcatcatc gagcctggga
     9961 ttatcgccaa agaggctcag ctcgtcgtca agctcagcga ccagcggggc cggcaccgag
    10021 cggttgagag cccggtcaag taccacaccg gcgtcggcaa cgtgcaaccg gccggaaagg
    10081 tagtcccaca ccgcttcggt tcgggttcga atccggcgat gccagcgggc acgctcgatc
    10141 aaggggtaga cgacttcggg ccataaggct gcgctggccg ggtcacgatg tgcattgctg
    10201 gtggcataac gtaaaactgc cgcgcggaac gcgacttcgt tacgccagac ctcggccacg
    10261 cggttccacc gttcgaccag aacatcatca accgcgtcgc gggccggcgc gatcaagttt
    10321 aggtcttcca gcttctgcgg catcaagccg gggttgagca cgcctttaga gtcaatcatg
    10381 tgtttttggg cgtacacact ggcctgggcg atggccttgc gggtctggcg gaagagcttg
    10441 cgcttggcgg ggctttcgcg gcaggtcagg tacttggcca ccacgatcgg cgacaatatg
    10501 ggacggactt cgatcttctg gttcaagagc gtagctgcat cgcggatcgc atcttcgccg
    10561 ccgccggcca ggattgcgta ggtcacgccg agaagcaatc ggccgcgatc cgcgttgcga
    10621 ggttccaacg gccggcgcag gaacaggccc cggcggcgcg agccgctcat caagtaggcg
    10681 agatagtggc catgaagata gcgctcgtcc ggagatagta gaatcggctc ggccaggacg
    10741 acctcgagca tccggcgggc caatggcgag cgaatgagcg cggtcacacc tccggttccg
    10801 ccaacggtcg caatggccac gggcgctggt gatgaagggc cgccctcctg tgggtgtttc
    10861 gatggggagg aaggaatggt cgccgcggtc ggcgcgggtg ggccagcgcc agtcacgagc
    10921 gtctcgccac taatcgtgtt gctcggtgaa gaagcggtaa gtgcgctgga agtccgtgtc
    10981 gctgtcaacc cgttggccgg ctggattgat tgcgcgggca aaggttcggt caccacgggt
    11041 gccgtcaagc cgaccgtacc tgtcaaattc gtagtgcccg cggcacctac gccgacggat
    11101 gctgtcgccg cactaccgcc ggatccatcg cgttcacgaa tccgcgcgac ctcctggcgc
    11161 agcgccagtc cagccagtgc tccggatcca ggccgctcgt cgggccgctt tgccagaagc
    11221 gccagaatca gagcctcaag ttcgggcgag acaaaaggat tgcgcgcccg aggcctttcg
    11281 ggattagtct ggtagtgctg agacatg
//
LOCUS       DATAQL010000300         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4457474, whole genome shotgun sequence.
ACCESSION   DATAQL010000300 DATAQL010000000
VERSION     DATAQL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4457474"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1471
                     /locus_tag="VHS97_05130"
     CDS             257..1471
                     /locus_tag="VHS97_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3447612.1"
                     /translation="MTVELSGVLVSESGPTSNVGGRGARYDGEPGRRTFLKGLGTLGA
                     ALSGGAIMTTAAAADDDRTISKGDAALLRFAAAAEIIESDLWLQYAELGGVQDDEVSL
                     LASQLIPGYPAKATGGNPTYIKGLQFLDGDMPQYIHDNTEDELTHEHFINAYLESKGA
                     EPVNLDKFRILHGSQATGANKSFPRLTNLTQLTVDTTWWTRYRSRTKNPDPPFNDTFP
                     PAIPTLATGRHTAIPRTDSDTTNGNFIQAVANTAGFHFAFIEQGGTSLYPGLAQRANS
                     VEVLRILLSIGGTEICHFQTWHDKAGNAKVLANVIDPVTHVSVTFPDLNTPPSEDLQT
                     NLIMPEPTVFLSRQFPPCSIIRPTETKGAAMAAVKSLTDDGLFIGQLSEFGELLEHLA
                     AEADAARRDGGD"
     gene            complement(1606..>1790)
                     /locus_tag="VHS97_05135"
     CDS             complement(1606..>1790)
                     /locus_tag="VHS97_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX3447613.1"
                     /translation="LVDDPMVAAEQFLALVRGELHLHCLYDPSFRPSRVQIERQIDAG
                     IDCFMARYGAAVTSSV"
BASE COUNT          375 a          564 c          518 g          333 t
ORIGIN      
        1 ataagtaggt ccacattttc acacttactg tcatgctaat gtcacagacc gatacttgtc
       61 accgttctta tagccgtcgt gatgtttagg ttcccggaga agttgcggcg aagacgcagc
      121 gcgtttttct caacgaataa gaatgctggc gccggtgttt attatgcggc ggggggcgga
      181 gctatacccg ccagcttcgg tagttccaga agcttgcgct tccgccggga aagtcttgcc
      241 aaaatggaga agcacgatga ccgtagaatt gtccggcgtt ctggtttccg aatctggtcc
      301 gacgtccaac gtcggcggcc gcggcgcgcg gtatgacggc gagcccggca gacgcacgtt
      361 cctgaaaggg ctcgggactc tcggcgcagc cctttcgggc ggcgccatca tgacaaccgc
      421 ggcggccgcg gacgacgata gaacgatcag taaaggagac gctgccctct tgcggttcgc
      481 cgctgcggca gaaatcatcg aaagcgacct ctggctgcaa tacgccgaat tgggcggcgt
      541 ccaggacgac gaagtctcgc tcctggcgag ccagctcatc cccggttatc cagccaaagc
      601 aaccggcggc aatccgactt acatcaaggg tttgcaattt ctcgacggcg atatgcccca
      661 gtacattcac gacaataccg aagacgagtt gactcacgaa cactttatca acgcgtatct
      721 cgagtccaag ggcgcggagc cagtgaattt ggacaagttc cgcattctgc acggcagcca
      781 agcgaccggg gcgaacaagt ccttcccccg gctgacgaac ctcacccagc tcaccgtcga
      841 cacgacatgg tggacgcgct accgcagccg gaccaagaac cccgatcccc ctttcaacga
      901 caccttccct ccggcgatcc cgacgcttgc aacgggccgg cacaccgcca ttccgcgaac
      961 cgactccgac acaaccaacg gcaatttcat ccaggccgtc gccaacaccg ccggcttcca
     1021 ttttgccttt atcgaacagg gcgggaccag cctctatccg gggcttgccc aacgcgccaa
     1081 cagtgtcgag gtcttacgca tactgctgag catcggcggc accgagatct gtcacttcca
     1141 gacgtggcac gacaaagcgg gcaacgccaa ggtcttggcg aatgtgatcg acccggtgac
     1201 ccatgtgagt gtgacgttcc ccgacctgaa tactccccca agcgaagact tgcagaccaa
     1261 cctgatcatg cccgagccga cggtgtttct gagccggcaa ttccccccgt gttcgatcat
     1321 tcgtcccacc gagacgaagg gggcggcgat ggctgcggtc aagtcgctta ccgacgacgg
     1381 gcttttcatc ggtcaattgt ctgaattcgg tgagctgctg gagcacttgg cggcggaagc
     1441 cgacgcagcg cggcgcgacg gcggcgactg aacgcttcag aagaagaacc cctggggctg
     1501 tgagccgcgg ctcggctcgc agccccggag ggagcatgcg ccgtgtaatc cggcttttgc
     1561 gcgtgggagc gccgcagcgc gctcagccca ctctccccgt ccggtctaga ctgacgatgt
     1621 caccgccgcg ccgtatcggg ccatgaagca atcgatgcct gcgtcgatct gccgttcgat
     1681 ttggactcgc gagggccgga aacttggatc gtacaggcaa tgaaggtgga gttcgcctct
     1741 cacaagagca aggaattgct cggctgcgac catcggatcg tcgaccagca
//
LOCUS       DATAQL010000301        11531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_640461, whole genome shotgun sequence.
ACCESSION   DATAQL010000301 DATAQL010000000
VERSION     DATAQL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11531
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640461"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..411)
                     /locus_tag="VHS97_05140"
     CDS             complement(136..411)
                     /locus_tag="VHS97_05140"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEX3447614.1"
                     /translation="METGSETTLDLRLPAALKEAVELAAAHLGQTVDEFAVAALAQTA
                     REVIEHGGETLLTDRDWDRFLSLLANLKSEPNHALRAAAGRYEKHFG"
     gene            complement(592..3393)
                     /locus_tag="VHS97_05145"
     CDS             complement(592..3393)
                     /locus_tag="VHS97_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3447615.1"
                     /translation="METIVTGKPGWVVAIWLVVAAAVGSFSPNLTKLAAEGQAKMLGS
                     DAESRRAADLVKQSWPDEAYESMAVAVLHRPSGLIDADRQYALALSSRFLSAERPSEV
                     VRVLGPASMPEIAQRLVSADRTTALVAVSLAKSFVAPVTQEVVWWMESQATSGELVVP
                     PGLELRWTGDAVIGRDYMANVQTSLDRAAVATVVLLLIVLLVVYRSFWLALVPLTTIG
                     VSLIIARGVLAWMILAGWEVSSLVELFLIAILFGTGTDFCLFLSWRYAEHLNPSNPAG
                     SMRVTLARSFAALVTSAGTIIIGLLLMGTTKFKLFSSTGPSVALALSLALAATLTLTP
                     SLLVLLARLHPRSFEGLAGSSREFWERLGRAAMARPLRSWVFTLLAMLPLSILGVNTH
                     FIQDLMTELPTSAASARDFRLVASRFEPGMLAPLTVVLESDADFRRSEGLALIDDVSR
                     LLSHQRQLSQVRSATQPLGSTEPLERARLTSRLGEVNAGFHQLADGAGQLQKGLTEGA
                     AKLRAAIWLEEKTGLSLTGKPTASLAPKSDPSVTRAHSGEAFASTLQQASAVLRWSKG
                     VTTAFNLPAINSAVAGMSGDRESPKSSNTTNSAAQTFRTPESARSSVEKPQEYLLREL
                     TRAADGAAQIAGGALRANREVTAILNDPVGRRALDRLLINDQTVQENPELKRSFAAYI
                     TPDGHRARLDLTQSNRMFSHDAMDQVLTLRRRLNDYLGEYQGIHVTAKVAGANAESAD
                     VRSLTHDDQVQSWFIVPIGVFIVLIVALRDPLACLNLVMTMVLTYAFALGTTHLVFVS
                     LLGAEGLDWKVPYFLFVLLIAVGVDYNVFLMARLNEESQKRGLRDGIIRAIGQTGGLI
                     SSAAMITASSFASFLFSPLGSLRQLGFALVVGILVDAMLVRPLLVPCGHWLLKRTREA
                     LSPHIVVRRGKREYAVIPD"
     gene            complement(3756..4268)
                     /locus_tag="VHS97_05150"
     CDS             complement(3756..4268)
                     /locus_tag="VHS97_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3447616.1"
                     /translation="MARPDPSEYVAHYESYVSLVPEEDILEAMRSELGRTVDFLTGVS
                     DREASICHPPYTWTAKQVVGHLTDCERIFGYRALRIARGDSTPLPGFDENTYAQTAEF
                     DQHPLTDLVAEFRTVRQSHLSLFQNLPPKAWDLRGTANGDGVSVRGLAYIIVGHERHH
                     AAILRQRLSR"
     gene            complement(4300..4704)
                     /locus_tag="VHS97_05155"
     CDS             complement(4300..4704)
                     /locus_tag="VHS97_05155"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3447617.1"
                     /translation="MPKNLLLAASLPALPDQLFDMYLDAATHTAFTGAPVTIAPRAGA
                     SFRAFDGALSGTILHVEPKRLIVQTWRSTHWAGDDIDSVLVLSFLPEKEGARIELAQI
                     NVSDNDFAGVSHGWEKYYWTPWRAYLLGISSS"
     gene            complement(4755..6125)
                     /locus_tag="VHS97_05160"
     CDS             complement(4755..6125)
                     /locus_tag="VHS97_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX3447618.1"
                     /translation="MFRDRATHKTATGGKALKVLNSSHILTLLVFIYILVFATAWGQS
                     RDLGAAAGREHGGVAKPGSDELVPVAVPEPSPKAMQYYRSGMWLWVLNRIWGLLLPGL
                     IAFSGLSARLRSLARRLGKFWFFTIGVYIVLYLALVFLIDLPLSYYQGFVRQHAYGLS
                     NQTVGKWLRDSLIGFSIEAAACFLFLWIPYLLLAKSPKRWWIYTAILMVPFLFLTVFV
                     KPIWIDPLFNTFGPMKKTELERSILSLADRAGIEGSRVFEVDKSVDTKAVNAYVTGFL
                     QTKRIVLWDTLIKKLEEHELLFVMGHEMGHYVLGHVVRSILLSSIVTLAGLFLIDRLG
                     RWLVNRYHDWLGFADLADIASVPLMIMLLELVFLVLSPLGLAYSRHQEHEADRFALEL
                     TRTNHSAGLSFVKLQGENLSNPRPGLFYRIFRSSHPSIGERIDFCNSYHPWLSDQSAS
                     ASEPAK"
     gene            complement(6136..7554)
                     /locus_tag="VHS97_05165"
     CDS             complement(6136..7554)
                     /locus_tag="VHS97_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX3447619.1"
                     /translation="MSGDEIIFGEMTVREPERRVRPDRDRKFTSLAYEIPGQADLPIF
                     LDRVAADAIERHALSDTSVELGGILLGKECLDQQTGQPFVWVTHSLEAKHYANTQASF
                     TYTHDSWEEITRQRDQRFPDHDIVGWYHTHPSFGIFLSHHDLFIHQHFFAQPLQVAYV
                     VDPINQTRGFFQWRDKAMAQVGGYYLTADRGDRLALARLVNDLERIPNSDQGSGGNLS
                     PRLEAELINMLTRPTTHRDTSSPVERAQLAATFGLAGIFLGLLIVAAAFWIARIHGLL
                     EEQRESVKGLSTQVEKSSQSLASFADQSSAERRLASDTLERVLSDKKTPKFDELYEKL
                     HTELRNANRKYETQLSANEALAARKKDLEGEAADLRTQLADTRKERDDFKIDALKAEE
                     LRNEIASLQKQAKDALDPSSNSVDGEKKNAIAQELVRTRYAAYGGWGCTALLGLLAAY
                     IYFKPVVEDTPGIDNPPTHRIE"
     gene            complement(7551..8753)
                     /locus_tag="VHS97_05170"
     CDS             complement(7551..8753)
                     /locus_tag="VHS97_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX3447620.1"
                     /translation="MDERPPTTPQPESLVIDDQDRYSRLRLISWWRQEKLQAAKVLVV
                     GAGALGNEVLKNLALLVLGTTYLIDLDLVELSNLSRSVLFREQDGGQPKALVATRRAL
                     ELNPEITIIPLHGDVITDIGLGLFAEMDLVIGCLDNREARLWVNRQCWKTGTPWIDAG
                     IQEIQGVVKVFVPPDSACYECAMTKRDYELLNYRYSCPLLTRDQITSGKVPTAPTIAS
                     MMAALEVQEALKLLHGMPVAAGSALVYNGVSNQFYSTRLPFRDDCLSHETYPDPIELE
                     LGSASTLDELFASARSHLTGPFSLVLDRELVVAIDCPRCSGRQEMMRPRVRVKASEAV
                     CPNCNEPGRPEFVSAIAEDSPLAAEPLSRLGIPPYDIVRVDGATESGFFLLAADRGWY
                     NKGWGTGR"
     gene            complement(8996..9973)
                     /locus_tag="VHS97_05175"
     CDS             complement(8996..9973)
                     /locus_tag="VHS97_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HEX3447621.1"
                     /translation="MHQSPRTRRLHNDLTALERLRSESSVFRFTAVGDPPQQYQLWFQ
                     GKSLWRDRAKVKPLESHRVEIKLGASYPRTVPELRWLTPIFHPNISEIGMVCLGGYGT
                     HWVPSVQLDDLCVMLWDMARYHNYDIRSPYNREAALWVASQTTFRFPLDDRPLRDLRA
                     ALGRSAVNGEQPPDADSNHRGKKKPLDSPPRRAPINGTTPVTRVLNFIERYSRVFGNV
                     IGTEASQGAGTGDVRRRPEFQHMRQILDRSAASPPPRVRAPQPETADSNPQPAISSQM
                     ADEQAIELADTNHTERAPERDELVILDENENNRPPDRVAGDGGGILYIE"
     gene            complement(10073..10393)
                     /locus_tag="VHS97_05180"
     CDS             complement(10073..10393)
                     /locus_tag="VHS97_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEX3447622.1"
                     /translation="MSYGGNAEPSGIQVTFLDQTGAKAVKAVIAFSVPVSRIIPNIIT
                     KMSLPATSPDGQPMSYSLDHKEGGRRLLENMTLVEANVQDGDHLIVYPEVVAGCAQLP
                     ALPG"
     gene            complement(10772..10951)
                     /locus_tag="VHS97_05185"
     CDS             complement(10772..10951)
                     /locus_tag="VHS97_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX3447623.1"
                     /translation="MSLLKWAFLFLVLAGIAGLFGFSGIAAGAEDIAKVLFMVFLVVF
                     AVIGILAVTAFKTVT"
     gene            complement(11111..11518)
                     /locus_tag="VHS97_05190"
     CDS             complement(11111..11518)
                     /locus_tag="VHS97_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447624.1"
                     /translation="MSLCRTRPIVGLGLFLAAASFAPAPSEKPPTISPLAEARYRAAV
                     KQFDETWAYYRQARIDSYQVYVWSRLVLESRRTIAVKPAEQITALEDHLDRMKKLEEL
                     VTKIRRLGFGRSYDVGASEYYRLEAESWLEQVK"
BASE COUNT         2365 a         3444 c         3530 g         2192 t
ORIGIN      
        1 cgtgccaggt ctctctggtc gaactgaccc gcacgctgct tcagccaatc attcagcgcc
       61 gcgtggccac agtcgaacgc ggctcggtca tggtcacgac cgagccttgc gatagtccag
      121 ttcccttccg gggcgctagc caaagtgttt ctcgtagcgc cccgcggcag ctctcagagc
      181 gtggttcggc tcggatttga ggttagccag cagggagagg aaacggtccc aatcgcggtc
      241 ggtgagcaga gtctccccgc catgctcgat gacttcacgg gcggtttgcg ccagtgctgc
      301 aacggcgaac tcgtccactg tttggccgag atgggccgct gccagctcca cggcttcctt
      361 tagcgccgcc ggaagccgaa gatctaacgt agtttcactg ccggtctcca tcgcgacaac
      421 tccgacttgc ggaacgctcc aggcttcggt cgatctggcc ccccgagacc attctatccc
      481 gagaaggttc ggtccgccta ccggaagcgc cctgcggaac caagcagccc cgcgccgttg
      541 tccgcaccac agtgggagcg gcgtcagttt tccggtgtcg cgagatccgg ctcagtccgg
      601 aatgaccgcg tactcccgct tccctcggcg gacgacgatg tggggcgaga gcgcctcgcg
      661 ggttcgcttc aagagccagt ggccgcacgg aaccaatagc gggcggacga gcatggcatc
      721 gaccagaatc ccgaccacga gcgcgaatcc gagctggcgg agtgatccaa gcgggctaaa
      781 gagaaatgag gcgaagctgc tggccgtgat catggcggct gagctgatca agcctcccgt
      841 ctgaccgatc gctcggatga tgccgtcgcg cagtcctctc ttttgagatt cctcgttcaa
      901 ccgcgccatc aggaagacgt tgtaatctac ccccaccgcg atcaagagga cgaacaggaa
      961 gtacggcacc ttccaatcga gcccctcggc gccgagcaga gagacgaaca ccaggtgcgt
     1021 cgtccccaga gcaaaggcat aggtcaagac catcgtcata accaggttca agcatgccaa
     1081 gggatcgcgc aaggcgacga tcaagacgat gaaaacaccg atgggcacga tgaaccagct
     1141 ttggacctgg tcgtcgtggg tcagcgagcg gacatcggcc gattcggcgt tggcgccggc
     1201 gaccttggcc gtgacgtgga tgccctggta ttcgccgagg tagtcgttca agcggcggcg
     1261 caaggttaag acctggtcca tcgcgtcatg cgagaacatg cggttcgact gagtgaggtc
     1321 gagccgcgcg cgatggccat cgggagtgat gtaggccgca aagctgcgct tgagctcggg
     1381 gttttcttgc acggtctggt cgttgatcaa aaggcgatcg agcgcgcggc ggccgacagg
     1441 atcgttcaag atggcggtca cttcgcggtt ggcacgcaat gcgccgccgg cgatttgggc
     1501 ggcaccgtcg gcggcgcgag tcagctcgcg cagaagatac tcttgaggtt tttcgacact
     1561 tgatcgggcg gattccggcg tacgaaaggt ctgcgccgcc gagttcgtcg tattgctgct
     1621 cttgggcgac tcacgatcgc cgctcatgcc ggcgacggcg ctgttgatgg cgggcaaatt
     1681 gaatgcggtg gtcacccctt tactccatcg cagcacggcc gaggcttgct gcaaggtcga
     1741 cgcgaacgcc tcgccggaat gggcccgagt cacagacggg tcggatttgg gggccagcga
     1801 cgccgttggc tttcccgtga gcgacaggcc agtcttctct tcgagccaga tcgccgcccg
     1861 cagcttggcc gccccttctg tgagcccctt ttgaagctgg cccgcgccgt ccgcgagctg
     1921 gtggaatccc gcgttgacct cgcccaaccg cgacgttaac cgggctcgct ccagaggctc
     1981 ggtacttccc aggggctgtg tcgccgatcg gacttgggag agctggcgct ggtgcgacaa
     2041 aagccggctg acgtcgtcga tcaaggccag cccctccgac ctgcgaaagt ccgcgtccga
     2101 ttcgagaacc acggtgagcg gagcgagcat tccaggctcg aacctggacg caaccagccg
     2161 gaaatcccga gccgacgcag cgctcgtggg aagctcggtc atcaagtcct ggatgaagtg
     2221 cgtattcact cccagaatcg acaggggaag catcgccagc aaggtgaaca cccagctacg
     2281 caagggacga gccatcgcag cccgcccgag ccgctcccag aactcacgcg agctgccggc
     2341 cagtccctcg aacgatcgcg ggtgcaatcg cgccagaagc accaaaagcg acggcgtcag
     2401 ggtcagcgta gcagccagtg ccagcgacaa cgccagcgcc acgcttgggc ctgtgctgga
     2461 aaatagcttg aacttggtcg tccccatcaa gagcagcccg atgatgatcg tccccgcgct
     2521 cgtgaccagc gccgcgaacg accgcgcgag ggtgactcgc atcgagccgg ccggattgct
     2581 cggattcaag tgctcggcgt accgccatga caggaacagg caaaagtctg tgccggttcc
     2641 gaacaagatc gcaatcagga atagctcgac aagcgacgaa acttcccagc cagccaggat
     2701 catccacgcc agaactcccc gggcgatgat caaactcaca ccgatcgtag tcagcgggac
     2761 aagcgccagc cagaacgacc gatagacgac caagaggacg atcaagagca aaacgactgt
     2821 cgccacggcg gcccgatcga gcgaggtctg cacgttggcc atgtagtcgc ggccgatcac
     2881 ggcgtcgccg gtccagcgga gctccaggcc cggcggtacg acgagctcgc cggaagtcgc
     2941 ttggctttcc atccaccaga ccacttcctg tgtcaccggc gcgacaaatg acttcgcgag
     3001 cgaaacggcg accagggcgg tcgttcgatc agcgctcacc agccgctgag cgatttccgg
     3061 catcgacgcc ggcccgagca cgcgcaccac ctcgctgggt cgttccgcgc taagaaaccg
     3121 cgacgagagc gcaagcgcgt attgccggtc ggcatcgatt aaaccgctgg gccggtgcaa
     3181 cacggccaca gccatcgact cgtaggcctc gtcgggccac gactgcttga ccagatcggc
     3241 cgcgcgccga ctctcggcgt ccgatccaag catcttggcc tgtccctcgg ccgccagctt
     3301 cgtcaagttg ggtgagaaac tgccgaccgc cgcggccacc acgagccaga tcgctacgac
     3361 ccagcccggc ttgccagtca caatcgtttc cattgcgtcc aaacagccac tccttgccgc
     3421 tgctcttaat gaaagctctc tccacgagca acgcacatcg cggggaatct gaacgcagcc
     3481 attgaccctt gatctttccc cgattcgatg agccgcctaa cctgcaatcg cctatccagt
     3541 tcatcacgcc tggccgtgcg ctcgagaacc tggcaacgct catagttgca ctttggcgct
     3601 cagttggaat gacagtcctt agtctagcga atctaggaaa tccaggcagg ctcatctgcc
     3661 ttttctatct tgtgtactat aggggaatat tctcccagat caagcacaat gtgtcatcgc
     3721 gatttcgtta acgggtaatc cgaggggcat gaagcctagc gcgacagtcg ctgacgcaga
     3781 atggccgcat ggtgtcgctc gtgaccgacg atgatgtagg cgagccctcg aacgctgact
     3841 ccatcgccat tggcggttcc tcgcaggtcc catgcctttg ggggcaggtt ctgaaagaga
     3901 ctgaggtgag attgcctgac cgtccggaac tcggcgacca ggtcggtgag ggggtgctgg
     3961 tcaaactcgg cggtctgagc gtaggtattt tcgtcgaagc cgggaagggg ggtcgagtcg
     4021 ccgcgcgcga tgcgcagcgc gcggtagcca aagatccgct cgcagtcagt gagatggccg
     4081 acgacttgtt tggcggtcca ggtgtaaggc ggatgacaga tcgaggcttc gcggtcggac
     4141 acgccggtca agaaatcgac agtccggcca agctccgaac gcatcgcctc gaggatgtct
     4201 tcctctggta cgagcgacac gtagctttca taatgcgcca cgtattcgga tgggtcgggg
     4261 cgagccattg cgattctcct tgccttgatg acttccgggt taactggaag agatcccgag
     4321 aagataagcc cgccatggcg tccaatagta cttctcccag ccatggctta caccggcgaa
     4381 gtcgttgtcg gatacgttga tctgtgccag ctcgatgcgc gcgccctctt tctcgggcaa
     4441 gaacgaaagc acgagcaccg agtcgatgtc atctccggcc caatgggtcg accgccacgt
     4501 ctgcacgatc agtcgcttgg gttccacgtg aagaatggta ccggaaagtg ctccgtcaaa
     4561 agcacgaaag gaagctccgg cgcgcggcgc gatggtgacc ggagcgcctg taaaggcggt
     4621 gtgggttgcc gcgtcgaggt acatgtcgaa gagctgatcg ggcaaggctg gtagtgaagc
     4681 cgcgagtaac aggtttttgg gcatgggagt gatctccggt cttcgattat ctgcgcccga
     4741 cgcgggctcc ctggctattt cgccggctcg gatgccgacg ccgactggtc ggacaaccac
     4801 gggtgatacg agttgcagaa atcaatgcgc tcaccgatgc tcgggtggct cgaccgaaag
     4861 atccgataaa acaggccggg acgcggattg ctgagattct ctccttgaag tttgacgaat
     4921 gaaagacccg cggagtgatt ggtccgtgtc agctcgaggg caaatcggtc ggcctcgtgc
     4981 tcttgatgac ggctgtaggc gaggcccaat gggctcaaga ccagaaacac cagctctaac
     5041 agcatgatca taagcggtac cgacgcaatg tcggcaaggt cggcaaatcc cagccagtcg
     5101 tggtaccggt tcacgagcca gcgccctagc cggtcaatta aaaagagccc agcgagtgtg
     5161 acgatcgatg acagcaggat cgaccgcacc acgtgcccca gcacgtaatg tcccatctcg
     5221 tgacccatta caaagagaag ctcgtgctcc tccagcttct tgatcagcgt gtcccaaagg
     5281 acaattcgct tggtctgcaa gaaacccgtc acataggcat tgaccgcctt ggtatcgaca
     5341 ctcttgtcga cctcaaagac gcggcttccc tcgatgcccg cccgatctgc gagtgacagg
     5401 atcgaccgct ccagctccgt ctttttcatc ggtccaaacg tattgaagag cggatcgatc
     5461 cagattggct tgacgaacac ggtcaagaac aggaatggca ccatgaggat cgcggtatag
     5521 atccaccagc gcttgggact cttggcaagc aacaaataag ggatccataa aaacaagaaa
     5581 caggcggcgg cctcgatcga aaatccgatg agagagtcgc gcagccattt acccaccgtt
     5641 tgattcgaaa gcccataggc atgttggcgg acaaacccct ggtagtagga caggggtaga
     5701 tcgatcaaga acacgagggc caggtacaag acgatgtaga cgccgatcgt gaagaaccag
     5761 aactttccca gccgccgcgc caggctgcgg agccgcgccg aaagccccga aaatgcgatg
     5821 aggccgggga gaagcaaacc ccagatccgg ttcagcaccc agagccacat gccgctgcga
     5881 taatactgca tcgctttggg gctgggctcg ggcactgcga ctggcacgag ctcatcggag
     5941 ccgggcttgg cgacgccgcc gtgttcccgg cccgccgccg cccccaggtc gcgggattgg
     6001 ccccaggccg tcgcgaacac gaggatatag ataaaaacca aaagcgtgag tatatgagat
     6061 gagttcaaga ccttgagagc ttttccaccg gtcgcggtct tgtgagtcgc tctatcgcgg
     6121 aacatggttt ggagctcact caatgcggtg ggtcggggga ttgtcgatgc ccggggtatc
     6181 ttcgacgacc ggcttgaaat aaatgtaggc ggcgaggagg cccagaagcg cggtacatcc
     6241 ccagccgccg taggcggcgt agcgcgtgcg gacaagctcc tgcgcgatcg cgtttttttt
     6301 ctctccgtcg accgagttgg atgatggatc cagcgcgtct tttgcttgct tttggaggct
     6361 ggcgatctcg ttccttagct cctcggcctt gagtgcgtct atcttgaagt cgtcgcgctc
     6421 ttttcgcgta tcggcgagct gagttcggag atccgcggct tcaccctcca gatctttctt
     6481 gcgagcggcc agcgcttcgt tggccgaaag ctgcgtttcg tatttgcggt tggcgttcct
     6541 gagttccgta tgaagctttt catacagctc gtcgaatttg ggcgttttct tgtctgagag
     6601 gactctttcg agtgtgtcgc ttgccagccg ccgctcggcc gatgactggt cggcaaagct
     6661 tgcgaggctc tgggacgatt tctcgacctg ggtcgacaac cctttgaccg attcgcgctg
     6721 ttcctcgagc aggccgtgaa tcctggcaat ccagaaagca gcagcaacga ttaagaggcc
     6781 aagaaagatc ccggccaaac cgaaggtcgc agcgagctga gcccgttcga caggcgagct
     6841 tgtgtcacga tgcgtggtgg gtcgagtcag catgttgatc agctccgcct cgaggcgtgg
     6901 agacaagttt ccgcctgagc cttgatcgga attgggaatt cgctccaggt cattgacgag
     6961 cctggccagc gccagccgat cgccccgatc ggccgtcaag taatagccgc cgacctgtgc
     7021 catagccttg tcgcgccatt gaaagaaacc gcgcgtctgg ttgatcgggt cgacgacata
     7081 agccacctgg agcggctgcg cgaagaagtg ttggtgaatg aacaggtcat ggtgagacag
     7141 gaagatcccg aagctggggt gggtgtgata ccaccccacg atgtcgtggt cagggaatcg
     7201 ctggtcgcgc tggcgtgtga tttcttccca cgaatcgtgc gtgtaggtaa agctggcctg
     7261 ggtgttggcg taatgcttcg cttcgagcga atgggtgacc cagacgaagg gttggccggt
     7321 ctgttggtcg agacactcct tgcccagcag gatgccgccg agctcgacgc tcgtgtcgga
     7381 aagcgcgtgg cgctcgatgg catcggcggc cacgcgatca agaaagatgg gcaagtcggc
     7441 ctggccgggg atctcgtagg ccaatgaggt aaacttgcgg tcgcggtcgg gccgcacccg
     7501 ccgctcgggc tcgcggaccg tcatctcgcc gaagatgatc tcgtcgccgc tcaacggcct
     7561 gttccccagc ctttgttgta ccaaccccgg tcggcggcta agaggaagaa acccgactcg
     7621 gttgccccgt cgaccctcac gatatcgtaa gggggaatgc cgaggcggga aagcggttcg
     7681 gcggccaggg gcgaatcttc agcgattgca ctgacaaact cggggcggcc cggctcgttg
     7741 cagttggggc agaccgcctc cgacgccttg acgcgaaccc gcgggcgcat catctcttga
     7801 cgccccgaac agcgcgggca atcgatcgcg acgaccagct cgcggtcgag caccagactg
     7861 aatggccctg tgagatgcga cctggcggac gcgaaaagct cgtcgagcgt gctcgcactt
     7921 cccagctcga gctcgatcgg gtcgggatat gtttcatggc tcaaacagtc atcccgaaat
     7981 ggcaaacgcg tcgaatagaa ctggttggag acgccattgt agaccagggc ggaccccgcc
     8041 gcgacgggca taccgtgcaa aagcttgagt gcctcctgga cctcgagtgc ggccatcatc
     8101 gaggcaatag tgggggccgt cgggactttc cccgaagtaa tctgatcgcg cgtcaagagg
     8161 gggcagctat agcggtaatt caaaagctca tagtcgcgct tggtcatcgc acactcgtag
     8221 caggccgagt cgggagggac aaaaaccttg accactccct ggatctcttg aatccccgcg
     8281 tcgatccagg gcgtgccggt cttccagcac tgccggttga cccaaagccg ggcctcgcgg
     8341 ttgtccagac agccgatgac cagatccatt tcggcaaaaa gtcccagccc gatgtcggtg
     8401 atcacgtcac catgcaaagg aataatcgtg atttccggat tgagctcgag cgcacgcctc
     8461 gtcgcgacga gggctttggg ttggccgccg tcttgctcgc gaaacagcac cgaccgcgac
     8521 aggtttgaga gctcgactag gtccagatca atcaggtagg tcgtgcccag gacaagtagt
     8581 gccagattct tcagaacctc gttgccaagg gcaccagcgc cgacaaccaa aaccttcgcc
     8641 gcctgcaact tttcctgccg ccaccaggat atcaggcgca agcggctgta gcggtcttgg
     8701 tcgtcgatta ccagcgattc aggctgggga gtcgtgggcg gtcgttcatc catcgtcacg
     8761 gtactcgtcg caatccgctc gagtttccca cggcgagagc gaacacattg caggggccaa
     8821 gaggcgctgg ttgcaagctg cgtgctttgt agcgctcgac tggaagacga cgtcgcgctg
     8881 cgaaaacggg aggaacgccg gagtgccgga aggacaactt ttcatcagcc cacacatcgc
     8941 ctggtcctgt ttctgcgaag gccacgacgc ccgcctcgac gatcttcgcg gatctttact
     9001 cgatgtagag aattcctcca ccatccccgg ccacccgatc cggtggacga ttgttctcgt
     9061 tctcatcgag aatgactaac tcgtctcgct caggggcgcg ctcggtatgg ttcgtatccg
     9121 ccaactcgat ggcttgttcg tcggccattt gactcgaaat tgcgggctgg gggtttgagt
     9181 cggcggtttc gggctggggc gcacggacac gcggtggcgg cgaggcggcg gatcgatcaa
     9241 ggatctggcg catgtgctga aactcggggc gtcggcgtac gtcgccggtt ccggcacctt
     9301 ggcttgcctc cgttccgatc acattgccaa agacgcgact gtaacgttcg atgaaattaa
     9361 ggacgcgggt cactggcgtg gtgccgttga tcggtgcacg ccgggggggc gaatcaagag
     9421 gcttcttttt gccgcggtgg ttcgaatctg catccggcgg ctgctcgccg ttaacagcgg
     9481 aacgcccgag cgcggctctc aaatcgcgaa gcggccggtc gtcgagcggg aaccggaaag
     9541 ttgtctggct ggcaacccaa agcgcggcct cgcggttata gggagatcga atatcatagt
     9601 tgtggtaccg agccatgtcc cagagcatca cgcacaggtc atcaagctgg acactgggta
     9661 cccagtgtgt gccgtatccc cccaaacaaa ccataccgat ctcggaaatg ttggggtgaa
     9721 agattggggt caaccagcgg agctccggca cggtacgagg ataagaggcc ccgagcttga
     9781 tctcgacgcg atgcgactcg agcggcttga ccttggcgcg atctcgccac aggcttttgc
     9841 cctggaacca gagttgatac tgctgcggtg gatcgcccac tgcggtgaac cgaaagaccg
     9901 agctctccga cctgagtcgc tcaagggccg tcaggtcatt gtggagacgg cgggtacgcg
     9961 gggactggtg catggtctgt cgagagccct gacgcgagac gactgcctcc cggtaagccg
    10021 cgatcagtga accgcctttg cggttcccag gaggcgatag gacattggcg gttcagcctg
    10081 gcagcgccgg caattgggcg caacccgcca cgacctccgg ataaacgatc aggtgatcgc
    10141 cgtcttgtac attggcttcc accagcgtca tgttctccag caggcgccgg ccaccctctt
    10201 tgtgatcgag agagtagctc atcggctgcc cgtcggggct ggtcgcgggc aagctcatct
    10261 tggtgatgat attggggata atccggctca cgggcacgct gaaggcgatg accgccttga
    10321 ccgccttggc cccggtttga tccagaaacg tgacctgaat tccgctcggt tcggcatttc
    10381 cgccgtatga catggtcagg atacctcctc agcattcatt cggcggtcag cgacgaatat
    10441 tgtcattgca gcgagcgttt tcggccgtcc cgcgggccgt gagcccgtcg aacaatttcc
    10501 tcccctccag aataacgctc ttgcgctcgc gtcgaaacag gctgctcacg gatttcggca
    10561 atcgtccctg ggatagtgaa gccgcctacc aaatgcaaat tgccgggatt ggacgggttg
    10621 tgtcccgccg tcccggcaaa accgcagatg cgtgcgttgg cacaaaccgc ggattcgtcg
    10681 cgtatttgtt gactctccgc aacgcgaggg aacagattaa aggaacaacg cgaaccgagt
    10741 cggccggacc cctggcaaac tcgcagtggc ttcaggtgac tgtcttgaat gcagtcactg
    10801 ccaggatacc gataactgcg aagactacca ggaagaccat gaaaagtacc ttggcgatat
    10861 cctcggcccc cgcggcgatt ccgctgaacc cgaataaacc ggcaatgcca gcgagcacga
    10921 gaaacaggaa agcccacttg agcaaagaca taataacctc cattccggtg tgaaattggc
    10981 acatgcagcc caagctctga cggggaacca aaagcggttg ccagccacgc cagagtacac
    11041 gccggtacat caagcaaaag gggtgccaag tcttatcgcc acggtcgact ggcgagctga
    11101 aacggcgccg tcacttgact tgctcgagcc agctctcggc ctcgaggcgg taatattccg
    11161 acgcgccgac gtcgtacgac cggccaaatc ccaatcgccg tatcttcgtg acgagctctt
    11221 ccagcttttt catgcggtcc agatgatctt cgagcgcagt gatttgctcg gccggtttca
    11281 cggcgatggt gcgtcgagat tcgagcacga gtcgggacca gacatagacc tgatacgagt
    11341 cgatccgcgc ttgccgatag tatgcccaag tctcgtcgaa ttgcttgacg gccgcgcgat
    11401 aacgcgcctc tgcaagggga gaaattgtgg gcggtttctc ggacggcgcg ggggcaaacg
    11461 acgctgccgc caagaatagg cccagcccaa caatcgggcg cgttctgcac agtgacatga
    11521 tcccctcctt g
//
LOCUS       DATAQL010000302         7618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2014271, whole genome shotgun sequence.
ACCESSION   DATAQL010000302 DATAQL010000000
VERSION     DATAQL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014271"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VHS97_05195"
     CDS             <1..821
                     /locus_tag="VHS97_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447625.1"
                     /translation="GELPERPYDLAAPPRLVIVHRHRLTAPDAQRLKGYRDPKAPSTA
                     PVIILCVSPYVRYDELERWSGLANHVLFEATAGDVLPRYVARLVEGRESRIARVDSTG
                     FRIEVAGSNRDLCEGLVEACVIAGYRAIAVDDLDAAGSVGAQPVAPQAVERALTIWDV
                     PVLEPDWPDRLEQRARSTGPVIAVFGFADREVVSTARARGAVACLELPYDVDDLIDVI
                     DRAGRSFPADKWPVRVRVEPAHRLPPRPRRRKQQHDNTTAAVPWSDRERKPTIS"
     gene            920..4186
                     /gene="mfd"
                     /locus_tag="VHS97_05200"
     CDS             920..4186
                     /gene="mfd"
                     /locus_tag="VHS97_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX3447626.1"
                     /translation="MPKSSSRSTKGTPIRELKDLTREFQRAPGFPEVVAALKNGRSAT
                     VDGAWGSASALVSAALALHAPASLVILLAHVGDVDDFRDDVATFAGIVPEVFPAWEKL
                     PRELKAADEVFGRRMRVLKKLASPNPPRLIVAPFQATLQPVPKPAALAKMSRTVVVGE
                     AVPIENLTAWLLERGMSRAEVVEVAGEFSVRGGILDVFPTDSTDPVRLEFFGDEIESI
                     RPFDVESQRSLDRWNSVTLTAALALEEADPASFGHPVDYFPEGTWVTLLEPADLREEG
                     RHYLGRIEDMRGLFTVERSFERLIRFPTIALSSLAADSLEATCHLRIESIERFSGEHT
                     KVKAELESAAAGENVLIACHNAAEVERLGEVFSDTELAQSKRLHLTVGRIRAGFHLID
                     AQTLVIGDHELFARAEIRRPVTRRRYESRAIESFLDLNEGDLVVHVNHGIARYRGLHF
                     VDKSAEHAEETLLLEFAEGTKLYVPIAKIDLVQKYVGGGKADPTLSKMGTSTWDKRKK
                     RVAEAVVDLAQELIDIQAARALRPGFVYPAEDSHWMAEFEAAFPYEETPDQLAAIESV
                     KHDMAQAKPMDRLICGDVGYGKTEVAIRAAFKAVDAGKQVAVLVPTTILAEQHHRTFS
                     ARMAEFPFTIEAVNRFRPKPEIREVLKRAGGGGVDILIGTHRILQKDVAFKDLGLVII
                     DEEQRFGVEDKEWLKTLRATVDVLTLSATPIPRTLHMSLLGIRDISNLETPPPDRKAI
                     ETRVTRFDREIVKRAILRELNRDGQIYFVHNRVYDIQSIADLVQSLVPEARIGVAHGQ
                     MHGEELERTMLAFIKRKYDILVATTIIESGLDIPNVNTIFINEADKYGLADLHQLRGR
                     VGRYKHRAYAYLLLESSKSVTPNAVKRLKAIEEFTALGAGFKIALRDLEIRGAGNILG
                     AEQSGHIESVGYELYCSLLESAARALTNQPDKPALDCSVELAWRAYLPRDYVPAARIK
                     LELYRRLGRLRTLNHLADFRQEMIDRFGPLPHPAENLLVEAEIRIMAGAWKLERVHVE
                     QGQYVVLTYRDRTRIEALARRHPGLVRVVDARKSYVPLGEDPVKTPEIAERVRTLLRS
                     "
     gene            4811..6340
                     /locus_tag="VHS97_05205"
     CDS             4811..6340
                     /locus_tag="VHS97_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447627.1"
                     /translation="MTTSESPRAHGSGGFSPAQGPLPESDPFFIRDRNTAKSRRTKAQ
                     GLLRRLAGRWLQILMLWLLISSVAMFLVHALVKPTYVAVSLLRVEQPDPDIFGPLKRL
                     SDDSRNLINLQTQVNVITSNAVLEPALANPAVANLPVIKGAEDPRQSLREKLKVEIIG
                     DTNLIRIASELADPKAAVAIVRAVTEAYISQSVDFDRLENRFRTEAYERQLAIISKEI
                     DSRKQTLKDLIKQRGAPAIIPLGMLNPKTETDPTQPTLNKVTEEQFAKLIDKQVQCDL
                     DYLDALAQIEAVKAVRERNQDKIDEELKSRVADVFKRDPKVAGLMDQMREARALAKSE
                     DQVPTPTALSARARLAKLSKDYEELWASESRRIHAQLAVEDHGPLSEARIRDLEVAVE
                     KARRKKESFAKQVEQVEVIRTEVDQNSFDFRHVNDQLESLMRSENQVRKNLEQLKDMA
                     AHQRNRVRILEEASASKSPKSGMLMRYMVAVPCVVLLLLVGSFLAHEIRADRRAATPL
                     D"
     gene            6442..7110
                     /locus_tag="VHS97_05210"
     CDS             6442..7110
                     /locus_tag="VHS97_05210"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX3447628.1"
                     /translation="MPVRSPDVPLVLMVLFSAGLFLGLPVWGWGSFDGFVDHPARVGA
                     CLVVVLASIPVLFSGTSLGRGMFSDARTPWIFLLAFGFTTVMAWLPAYADRRDIATLD
                     GDLSRYSGLALLILGCILRVGPMFELGPRFRPPWVKQEDHRLVTTGFYRYVRNPSYLG
                     ALLGMMGWFLVFRCGPGFLLSLLSIPVAIPLIRREEAMLQEEFGDAYLAYKNRTWRLI
                     PFVN"
     gene            7142..>7618
                     /locus_tag="VHS97_05215"
     CDS             7142..>7618
                     /locus_tag="VHS97_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447629.1"
                     /translation="MDDENPYAPPKYAQLDRDLVGEHPNAAWRDGRLLMARKGAVLPD
                     RCLKCNAPAEGRPFKRSLSWASPYWALSIFLIGPILFIVVYTIVSRRGKVSVSLCPRH
                     RMERLRAIALGWLAALAGIGCMIATGVASDYITPFGISLNLFLSIGIGFGIVLILAG"
BASE COUNT         1472 a         2299 c         2326 g         1521 t
ORIGIN      
        1 cgggcgagtt gcccgagcgt ccgtacgacc tcgctgcgcc cccccggctc gtcatcgtgc
       61 atcggcatcg actaacagcg cccgacgccc agcgattgaa aggctaccgc gatcccaagg
      121 cgccctcgac cgcgccagtg atcatcttgt gcgtcagccc ttacgttcgt tatgacgaac
      181 tcgaacgctg gtcgggactg gcgaaccacg ttctttttga ggccacggcc ggcgacgtcc
      241 tgccccgcta tgtcgcccgc ttggtcgagg ggcgagaaag ccggatcgca cgggttgatt
      301 cgaccggttt tcgcatcgag gtggcgggaa gtaaccgcga cctgtgcgaa ggcctggtcg
      361 aggcttgtgt catcgccggg taccgggcga tagcggtgga tgatctcgac gcggcaggta
      421 gcgtcggcgc ccagcccgtc gccccacaag cggttgaacg agctctgacg atctgggacg
      481 ttccggtgct cgagcccgac tggccggatc gcctcgagca gcgagcgcgc tcgaccggtc
      541 ccgtgattgc tgttttcggc ttcgccgatc gagaagttgt gtcgaccgca agagcacggg
      601 gggcggttgc ctgtctcgaa cttccctacg acgtcgacga tctgatcgat gtgatcgacc
      661 gcgccggccg gtcatttccc gccgacaaat ggccggtgcg cgtccgagtc gagccggctc
      721 accgtctgcc gccgcgtcca cgccggcgca aacagcagca cgataacacc accgcggcgg
      781 taccgtggtc agatcgcgag cgaaagccta caataagctg acattcggac acgagggaac
      841 cgcaagtgcc gtatggcgag tcgctcgtga gatcgttgtg accggtgacc ggtcactcgc
      901 gacacgcctc tggtgagcca tgcccaagag ttcgagtcga tccacgaagg gcacccccat
      961 cagggagctc aaggacctga cgcgtgaatt ccagcgcgcg ccgggttttc ccgaagtcgt
     1021 ggcggccctc aaaaacggcc gcagcgcgac ggttgacggc gcctggggct cggcgtcggc
     1081 cctggtgtcg gccgccttgg ccttgcacgc gccggcgtct ctggtgatct tgctggctca
     1141 cgttggcgac gtcgacgact ttcgcgatga cgtggccacc ttcgcgggaa ttgtcccgga
     1201 ggtgttcccc gcctgggaaa agctgccgcg cgagctgaaa gccgccgatg aggtatttgg
     1261 ccggcggatg cgggtcttga aaaaactcgc cagcccaaat ccaccgcggc tcatcgtcgc
     1321 cccatttcaa gcaactctgc aaccggtccc caaacccgcg gccctcgcca agatgtcgcg
     1381 caccgtcgtc gtgggcgaag ccgtgcccat cgaaaacctc acggcctggc tgctcgagcg
     1441 cggcatgtcg cgggccgagg tcgtcgaggt ggccggtgaa ttcagcgtcc gcggcggtat
     1501 tttggacgtc ttccccaccg actcgacgga cccggtccgg ctcgagtttt tcggcgatga
     1561 aatcgagtcg atccgcccct tcgacgtcga gtcgcagcgc tcgctcgatc ggtggaactc
     1621 ggtgacactc accgccgcac tcgccctgga agaggccgat ccggcgagct tcggccatcc
     1681 cgtcgattac tttcccgagg ggacgtgggt gactttgctc gagcccgccg atctccgcga
     1741 ggagggtcgt cattacttgg gccgtatcga agacatgcgt gggctcttta cagtcgagcg
     1801 cagcttcgag cgtttgatcc gctttcctac gattgccctt tcgagtctgg cggccgactc
     1861 gctcgaggcg acttgccacc tgcggatcga gagcatcgag cggttctcgg gggagcacac
     1921 caaggtcaag gccgagctcg aaagcgcggc cgcgggtgaa aatgtcttga tcgcttgcca
     1981 caatgcggcc gaggtggaac ggctgggcga ggtcttttcc gacacggaac tggcccaatc
     2041 gaaacggctc catctcaccg tcggccggat cagggccggt ttccatctga ttgacgccca
     2101 gactttggtg atcggcgacc acgagctttt tgcccgcgcc gagattcgcc ggcccgtcac
     2161 ccgcaggcgg tacgaaagcc gggcgatcga gagtttcctc gacttgaacg agggggacct
     2221 ggtcgtccac gtcaatcacg ggatcgcgcg ctatcgcggg ctccactttg tcgacaagtc
     2281 ggcagagcac gccgaggaaa ccctgctgct cgagtttgct gaagggacca agctctatgt
     2341 cccgatcgcc aagatcgacc tggtgcagaa atacgtcggc ggcggcaagg ccgaccccac
     2401 gctttcgaag atggggacat cgacctggga taaacgcaag aagcgagtgg cggaggcggt
     2461 cgtcgacctg gcacaagagt taatcgatat ccaggccgcg cgggcccttc gaccgggttt
     2521 tgtctatccg gccgaagaca gccattggat ggccgagttc gaggcggctt tcccctatga
     2581 agagacgccc gaccagctcg ccgccatcga gtcggtaaag cacgacatgg cccaggccaa
     2641 gccgatggac cgcctgatct gcggtgacgt cggctacggc aagacggagg tcgccatccg
     2701 ggcggcgttc aaggccgtcg acgccggcaa acaggtcgcc gttctcgtgc caacgaccat
     2761 cctggccgag caacaccacc gtacattctc ggcccgcatg gccgaatttc ccttcacgat
     2821 cgaggccgtc aaccggtttc gccccaagcc agaaatacgc gaagtactca agcgcgctgg
     2881 cggcggcggc gtcgatatcc tgattggcac ccaccggatc ttgcaaaagg atgtcgcctt
     2941 caaagacctg gggctggtga tcattgatga agagcagcgg tttggcgtcg aggataagga
     3001 atggttgaag acactgcgag cgaccgtcga cgtcctgacg ctttcggcca ccccgattcc
     3061 gcgcaccttg cacatgagcc ttttgggaat tcgcgatatc tcgaatctgg aaacgccccc
     3121 gcccgaccgc aaggcgattg agacccgcgt gacccggttt gaccgggaaa tcgtcaagcg
     3181 cgcgattctg cgtgagctca atcgcgacgg ccagatctat ttcgtccaca atcgggtcta
     3241 cgatatccag tctatcgccg acctggtcca gtcgctcgtc cccgaggccc ggatcggcgt
     3301 cgcccacggc cagatgcacg gcgaagagct ggagcggacg atgctggcat tcatcaagcg
     3361 caaatacgat atcctggtcg cgaccacgat catcgagagc ggtctcgaca tccccaacgt
     3421 caacacgatc ttcatcaacg aggccgacaa gtacggcctg gccgacctgc atcagcttcg
     3481 cggccgggtc ggccgctaca agcacagggc gtacgcctat ctgctcctcg agtcgagcaa
     3541 atcggtcacg cccaacgcgg tcaagcggtt gaaagcgatc gaagaattca ccgcgctcgg
     3601 cgccggcttc aagattgcac tccgcgacct ggagatccgg ggtgcgggca atatcctggg
     3661 cgcggagcaa tcggggcaca tcgagagcgt tggctacgag ctctactgtt cccttctgga
     3721 atcggcggcc cgcgccctga ccaatcagcc cgacaagccg gcccttgatt gctcggtgga
     3781 attggcctgg cgggcgtatc tgccccgcga ctacgtcccc gcggcccgga tcaagctcga
     3841 gctttaccgc cggttaggcc ggctgcgcac gctcaaccac cttgccgact tccgccagga
     3901 gatgatcgac cggtttgggc ccctgcccca cccagccgaa aacctgctcg tcgaagccga
     3961 gatccgcatc atggccggcg cgtggaagct cgagcgggtt cacgtcgaac agggccagta
     4021 cgtcgttctg acctaccgcg accgtacccg gatcgaagcc ctggcgcggc ggcatccggg
     4081 attggttcgg gtcgttgatg ccaggaagtc gtatgtccca cttggtgaag atccggtcaa
     4141 gacgcctgag atcgctgaac gggtgaggac gctgctgcgc agttgaaaga ataggcagga
     4201 ggcaattggt ccttggtcct tggaccttgg tcattggtcc ttggtccttg gtccttggtc
     4261 cttggtcctt ggtccttggt ccttggtcct tggtccttgg tccgtggtca gtggtccttg
     4321 gttattggtt ttagccaaag ggcagcctgg ggagacaata cctcgcgcgg gggcaaaagc
     4381 gacgtttgag gacgggaggg cgaggctccg gccgagccgc ttcccatccc gtgtcggctc
     4441 agcggaggtt gagaattcgg gatttcggcc cgaagggcca gaaacacggc agcccagggc
     4501 gacgccctgg ggagcccgaa tcagtgcaaa aaaccctgcc ctgaaagggc ccgacactgt
     4561 gttcgatccc attatggacc gatctggagg tctgggtatt caataatggt atcccgtacg
     4621 tttgccgaaa tccgcgatcg cacgcgaatg cgacgcatgt cgctcatcaa aatctggttc
     4681 agcggtgttg actctgatac gatcagataa attcacgaca tatttggaat gtgtctcgaa
     4741 tctacaggtc tacgtcgcga gccacgattt cgatttcttc gagtcttcaa cattaattga
     4801 gctcatatcc atgaccacaa gcgaatcgcc ccgagcgcac ggctccggcg gattttcgcc
     4861 ggctcaaggt ccgcttcccg agtcggatcc gttctttatc cgcgaccgga acacggcgaa
     4921 gtcgcgccgg accaaggctc agggcctgct gcgtcggctt gctggccgat ggttgcaaat
     4981 cctgatgctc tggttattga tttcctctgt ggctatgttt ctcgttcatg cgctcgtcaa
     5041 accgacctac gtggcagtca gcctcttgcg agtcgagcag ccagacccgg atatattcgg
     5101 gcccctgaag cgtctcagcg acgatagtcg aaacctgata aatctccaaa ctcaagtcaa
     5161 tgtgatcact tccaatgctg tgctcgagcc ggcgctcgcc aatcctgcgg tggccaacct
     5221 gcctgtaata aagggggctg aagatccccg gcaaagtctc cgcgagaaat tgaaggtcga
     5281 gatcatcggc gacacaaatc tcatccgcat cgcctcggag ctggcggacc caaaggcggc
     5341 cgtcgcgatc gtcagggccg tgactgaggc gtacatttca cagagcgtcg acttcgatcg
     5401 cctcgaaaac cgctttagga cggaagccta tgaaagacag ctcgcgataa tctccaaaga
     5461 aatcgattcc aggaagcaga ctctcaaaga cttgatcaag caacgagggg ccccggcaat
     5521 cattcctttg ggaatgctga atccgaagac cgagaccgat cccacgcagc cgactttgaa
     5581 caaagtcacg gaagagcaat ttgccaaatt gattgacaag caggttcagt gcgatcttga
     5641 ttatctcgat gctctggccc agatcgaggc ggtcaaggcg gtccgagaac ggaatcagga
     5701 caagattgac gaggaactga aaagccgcgt tgccgacgtg ttcaagcgag atcccaaggt
     5761 ggctggtttg atggaccaga tgcgtgaggc acgtgcgctc gcgaaatccg aggaccaagt
     5821 cccaacgccg accgcactat cggctcgggc gaggcttgcg aagctctcaa aggactacga
     5881 ggaactttgg gctagcgaat cccggcggat ccacgcgcaa ctggcggtcg aagaccacgg
     5941 cccgttatcc gaggccagga ttcgagatct ggaagttgcg gttgagaagg cgcggagaaa
     6001 gaaagagagt ttcgcgaagc aggttgaaca ggtcgaggtg atccgtacgg aggtggatca
     6061 gaactcattc gatttcaggc acgtcaatga tcagctcgag agcctgatgc gctcggagaa
     6121 tcaagtccgg aaaaacctcg agcaattgaa ggacatggcg gcccaccaga gaaatcgcgt
     6181 gaggatatta gaagaggcct cggcttcaaa atcccccaaa agcggtatgc ttatgcgcta
     6241 catggtcgcc gtgccgtgtg tcgtcctgct gttgcttgtc ggatcgtttc tcgcacacga
     6301 gataagggcc gacagaagag cggcgacacc gctcgattga acgccgcacc gcaagcgaat
     6361 ctggcaccca tcaacggcgg acccggggga acatctccat tgctcgtcgg ccaattccga
     6421 tgacctcaag agaagatcgc gatgccagtg cgctccccgg atgttcccct ggttctcatg
     6481 gtccttttct ctgcggggct tttccttggt ctgcctgtct ggggctgggg aagcttcgac
     6541 ggttttgtcg atcatcccgc gcgcgtcggt gcctgccttg tggtcgtcct cgcgtcgatt
     6601 cccgtgctgt tttcgggcac aagcctcggc cgtggcatgt tctccgatgc tcggacgcca
     6661 tggattttcc tgctcgcgtt cggattcacg acggttatgg cctggttgcc ggcctacgcc
     6721 gaccgccgcg acatcgccac gctggatggc gacctcagcc gttacagcgg tctggcgcta
     6781 ttgatcctgg gctgtatctt gcgcgtcggg ccgatgttcg agctgggacc gaggttcaga
     6841 ccaccttggg tcaaacagga ggatcatcgg ctcgtgacga cgggcttcta tcgctacgtt
     6901 cggaatccca gttatttggg cgcgttgctg ggaatgatgg gctggtttct ggtcttccgt
     6961 tgtggaccgg gctttttact atcacttcta tcgatcccag tggcaatccc attgatccgc
     7021 cgggaagagg ccatgctgca agaggagttt ggtgatgcgt acctcgccta caagaatcgt
     7081 acgtggcgat tgattccgtt tgtcaactga tggtttccct cgtgcggaga actcggcagt
     7141 gatggacgac gaaaacccct acgctccccc aaagtatgcg caactcgacc gcgacctcgt
     7201 cggggagcat cccaacgcgg cttggcgcga tggacggctg ctgatggcgc gaaagggagc
     7261 ggtactgccc gatcgatgtc tgaagtgtaa cgcaccggct gaggggcgtc cgttcaaacg
     7321 ctccctttcg tgggcgagcc cttactgggc cctgtcgata ttcctgattg gcccgatctt
     7381 gtttatcgtc gtttacacca tcgtgagccg gcgaggaaag gtctcggtga gcctttgccc
     7441 acgccaccgc atggaacggt tgcgggccat cgcgctggga tggttagccg cgctggccgg
     7501 catcggttgc atgatcgcca cgggggtcgc ctcggactat atcacgccgt tcgggatttc
     7561 cttgaacctt tttttgtcga tcgggatcgg ttttggaatc gttcttatac tcgccggc
//
LOCUS       DATAQL010000303         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4303746, whole genome shotgun sequence.
ACCESSION   DATAQL010000303 DATAQL010000000
VERSION     DATAQL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4303746"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="VHS97_05220"
     CDS             complement(<1..1636)
                     /locus_tag="VHS97_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3447630.1"
                     /translation="MNPIVFALRRPITVMMLVLALALGGLFSVYQMQKDIFPSLNQPQ
                     LYVIHNYGGMDPKQIEGLITNVYELFFQYINGVEHVESRSIQSMVMLKLYFQPGIDMA
                     EATAQTVAYCNRALSVMPNGSLPPYVVRLDAGSLPVGYLVFESPTRTVGELQDMALFR
                     VRPMFSGLAGVSSPPPFGGNIRTIVVNVDPDRLRSYNLSPQDVVDAIDRGNFISPSGN
                     VTIKDQMTVVPTNSMVISPQELRNVPLKLGENVYIRDVGTIADATDIPVGYALVNNRK
                     SVYLPVVKQASASTLTVVNAVKANLSRFQAIMPDDVKVSYEFDESQIVYRAIESVAVE
                     GALGAGLTGLMVLLFLRDLRAVIIVVLNIPLALLGSIVILNLTGNTINIMTLGGLALA
                     IGILVDEATVEIENIHTQMEHTPSMARSVRQGNMETAVPRLLALFCILSVFIPSFIMQ
                     GAVRALFVPLSLAVGGAMITSYLLSSTFVPVLSVWLLENRHGTHGHEDGAETRPGFFV
                     RMQNGFEGVVDWTVAHRRKVVLAYLLATGAVVVVVGS"
     gene            1894..3099
                     /locus_tag="VHS97_05225"
     CDS             1894..3099
                     /locus_tag="VHS97_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3447631.1"
                     /translation="MRPSLNLIASVMLALSSALTMGSPGGMSWLGDSFSLVSGTVLSE
                     ARAESKNATRPASRTAFQFEPIGLSLLEPYARGKIPAVFVHGLGNTPDSWTRMIECLE
                     ADPTIRQHYQFWAFGFSTGEPILYSASLLRQALADARERFDPERTDPAFDRMVLIGYS
                     MGGILAKAMAQESQSTLWDEISTRSFATLQGPEEAREALRQSFFFKSVPEVSRVICIA
                     TPHRGSQVDQGALHWLCSRLNPPMERLRKFHESLVASNDPEFFHKSFQEKLQSSVDQL
                     AWQHPRLLALLKLTVSPRVKFHSIIADVNDPPGAGGTDGVVPYASSHLEGASSERIVR
                     GGHLCQANPLVIEESDRILKEHLATSDHGRTASFGAAAGALGPVAGVGADLASRAIKC
                     SCAACEVTH"
     gene            complement(3825..4070)
                     /locus_tag="VHS97_05230"
                     /pseudo
     CDS             complement(3825..4070)
                     /locus_tag="VHS97_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
BASE COUNT          816 a         1248 c         1246 g          778 t
ORIGIN      
        1 ggctgcccac gacgaccacg acagcacctg tggccagcag gtaggccagc accaccttgc
       61 gacgatgcgc gacggtccag tcgacgactc cctcaaatcc gttttgcatg cgcacaaaga
      121 accccggccg cgtttccgcc ccgtcctcgt ggccgtgtgt cccgtgccga ttctctaaca
      181 gccacaccga gagaaccggc acgaatgtgc ttgagagcag gtacgaggtg atcatcgccc
      241 cgccgaccgc cagcgaaagc ggtacgaaca gcgcgcgcac cgccccctgc atgatgaacg
      301 aggggatgaa taccgagagg atgcaaaaga gcgccagaag acgcgggacg gccgtttcca
      361 tgttcccttg gcggacggat cgggccatcg agggggtatg ctccatctgg gtgtgaatgt
      421 tctcgatttc gaccgtcgcc tcgtcgacca ggatgccgat ggccagggcc agcccgccca
      481 gtgtcataat gttgattgtg ttgcccgtca aattgaggat gacgatcgac cccaagagcg
      541 ccagcgggat gttgaggaca acgatgatga cggcccgcaa gtcccgcagg aacagcagca
      601 ccataagccc cgtcaggccg gcgcccagag ccccttccac tgccacgctc tcgatggccc
      661 gataaacgat ctgcgactca tcgaactcgt acgaaacttt gacgtcgtcg ggcatgatcg
      721 cctggaatcg cgacaggtta gccttgaccg cgttgaccac agtcagggtc gaagcggagg
      781 cctgcttgac cacgggcagg tagaccgact tgcggttatt gaccagcgca taaccgacgg
      841 gaatatcggt cgcgtcggcg atcgtgccga cgtcacgaat gtagacgttc tcccccaact
      901 tgagcggcac gttgcgcagt tcttgcgggc tgatgaccat ggaattggtc ggtacgacgg
      961 tcatctggtc cttgatcgtc acgttgccgg aagggctgat gaagttgccg cggtcgatcg
     1021 cgtcgaccac atcctggggc gagaggttgt aagagcgaag gcggtcgggg tcgacattga
     1081 cgacgatggt ccggatgttg ccgccgaacg gcggggggga cgaaacgccc gccaggcccg
     1141 agaacatggg ccggacccgg aacagcgcca tgtcctggag ctcgccgacc gtccgggtcg
     1201 ggctctcgaa gaccaggtag ccgaccggta aactgcccgc gtcgagtcgg accacgtatg
     1261 gcggcagcga cccattgggc atgaccgata gggcccggtt gcagtaggcg acggtctgcg
     1321 ctgtcgcctc ggccatgtcg attcccggct gaaaatacag cttgagcatc accatgctct
     1381 ggatcgaacg ggactcgaca tgctccacgc cgttgatgta ctgaaagaag agctcgtaga
     1441 cgttggtgat caagccctcg atctgcttgg ggtccattcc gccgtagtta tggatgacat
     1501 agagctgcgg ctggttaagg gagggaaaga tatccttctg catctggtag acgctgaaga
     1561 gcccgcccag cgccagggca agtaccagca tcatgacggt gatcggccgg cggagcgcga
     1621 agacaatcgg gttcattttg atctcgacaa ttgcgcttga tggactgagg tcgtgatcat
     1681 cttaaacgcc atagcccaac taatgatagg gagcccggca agttgttaac gccgctggcg
     1741 aagagtgtca tcggccggct actgccgccg ttcggcctgc gtattgcaca gcgatatcac
     1801 ggtaaaccgg ccgagacatc ggactgaacc gatccttgac gccgcgacgt agaatttagt
     1861 gttgggctct tagccttgcc aaggaacagc accatgcgtc cctcactaaa cttgatcgca
     1921 agtgttatgc ttgccttgtc ttcggcgctc acaatgggct ctcccggagg catgagctgg
     1981 ttgggcgata gcttttcttt ggtctcgggc acggtgctga gcgaagccag ggccgagtcg
     2041 aaaaacgcca cgcggcctgc gtcgagaacg gcctttcagt ttgagcctat cgggctctcg
     2101 ctccttgaac cctacgcgcg cggcaagata cccgccgtat ttgttcatgg gctggggaat
     2161 acacccgatt cttggacccg gatgattgaa tgcctcgagg ctgatccgac catacgacaa
     2221 cactatcagt tctgggcctt cgggttctcc accggcgaac cgattctcta ttccgcgtca
     2281 ttgctgcgac aagcgctcgc cgacgctcgt gagcgatttg accccgaacg aacggacccg
     2341 gcgttcgatc gcatggtctt gattggctac agcatgggag gcatccttgc caaggcgatg
     2401 gcccaggaga gccaatcgac tctctgggat gagatcagta cccgctcgtt tgcgactctc
     2461 cagggtccgg aggaggcccg cgaagccctg cggcagtcat tcttcttcaa gtccgtcccg
     2521 gaagtgagcc gcgtcatctg cattgccacc ccccaccgag gcagccaggt cgaccagggc
     2581 gcgctccact ggctctgctc gcggctcaac ccaccgatgg agcgtttgag gaagttccat
     2641 gaatcgctag tggcgagcaa cgaccccgag tttttccaca agtcattcca agagaaactc
     2701 cagagcagcg tcgatcagtt ggcgtggcag catccgcggc tcttggcgct actcaagctg
     2761 acggtaagcc ctcgcgtgaa gtttcattcg atcattgccg acgtcaacga ccctcctggc
     2821 gcggggggaa ccgacggcgt tgtaccctat gccagctcgc acttagaagg tgcaagctct
     2881 gagcgaatcg tccgcggcgg ccatctttgc caggcgaatc ccctggtgat cgaagaatcg
     2941 gatcgaattc tcaaggagca tctcgcgact tccgatcacg gtcggacagc atcgttcggt
     3001 gccgccgccg gcgcgctggg cccggtcgcg ggcgtcggcg cggatttagc atcacgcgcg
     3061 atcaagtgta gctgcgcggc ctgcgaggtg acccactgag ctgggtggcc caccgcccga
     3121 tagagctcaa actgggcggc attgtagtcg ttaacggcgg ccgagtattg ctcgaaagcc
     3181 gtattaagag caatcaaggc ggcgacgact tcttgcggcc gaacgatcag acgtaagagc
     3241 tcgccagcgg gccgcgtggt ctcccgtaga ccgatgaagt ttttgtcggc cgactccgtc
     3301 gcctgccaca gtcccatttc agtcacgggg acgcgtgccc gcgccgtttg cagccgcgcc
     3361 agcgcctggg ttacatcggc acgaacgcga aacagcgtgc gtgttacttc gatcgcggcc
     3421 aagtcctgct cggcccgccg ttgccggatg agacctatat tgccaatccc cgcattctga
     3481 agttgccaca cagcctggac ctcaaaaaag gccgagtgag cgttagcccc cagcccagca
     3541 ttaggaccgg ccgataaatt gccggctgcc agcaagccgg tgttcgtgga cggactgaaa
     3601 aggatcaggc tgggcaggaa cggtcgcttc ttctcctgct tcagccgcat gttggccgcg
     3661 gccaacagtt cccgctgcga tgcgatctcc ggccgactgt gaatcgcgat ggggacgagc
     3721 tcggccaccg tctggtcggc cgggatcaac gtaacctgaa cgaagggggg ctcaaccggt
     3781 tcgagcaacg tctccggatc aaggagcaaa acgttcgcaa gcgcagcgct tgcgacctgc
     3841 caatcccgca gcgcgagctc gcgtgactgc agcaagctct gaagctcggc cttcgccctg
     3901 ttgatttcca gcggagcgat cagggccgga gctaacccga tcgcgagatt gaccagccgg
     3961 cgggcccgct caattgtcgc gtcgactcca gcgagccttc ctctcgcatc ctgcagggtg
     4021 aagtacgcct ggctgacttg caacagcacg tcattccgcg cggtctgcac attcgctgtc
     4081 tgcgcggc
//
LOCUS       DATAQL010000304         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_182509, whole genome shotgun sequence.
ACCESSION   DATAQL010000304 DATAQL010000000
VERSION     DATAQL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182509"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="argB"
                     /locus_tag="VHS97_05235"
     CDS             <1..868
                     /gene="argB"
                     /locus_tag="VHS97_05235"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX3447632.1"
                     /translation="GKFTVIKLGGSVMEDPESLRALLVDVVFMQTVGMRPVIVHGGGK
                     AITVAMEKAGLTARFVKGRRFTDEPTLAIVARVLAEEINADIERHINKFGGRASGLHH
                     KTHQCLYGRRLWLDNGPEGPIDLGRVGEVTAVDVPPIENLCLAGVVPVLPSLAEDQQD
                     EGRLLNVNADTAAAAVAQALQAEKLVFLTDTTGILLDKNEPATLIRGLTPSGCHDLIA
                     QGVIDRGMIPKVEACLSSLEAGVLKTHIIDGRLPHALLLEIFTKSGIGTEIVRDENSP
                     SPTGHRPVLGRP"
     gene            1009..2223
                     /locus_tag="VHS97_05240"
     CDS             1009..2223
                     /locus_tag="VHS97_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3447633.1"
                     /translation="MSTTQLAHQSSQATIAQFERYVIANYRRYPVSLVRGEGSWIWDA
                     EGHRYLDFFPGWGCNLLGHCPPRVVEAVRDQVGQLIHVPNTWYMEAQGALAQALSERS
                     FGGQCFFCNSGAEANEAAIKLARAVGHPSGRTKIVTMEGGFHGRTYAALTATAQPKYH
                     IGFEPMVPGFVYVPYNDIAAVAAVLDDQTAAVMVEPIQGEGGINIPSATYLGELRRLC
                     DDHGVLLILDEVQTGMGRTGEWFAYQHGGVVPDILTCAKALAGGVAAGVMIARPAVAA
                     VLKPGMHASTFGGNPIACRAALAAIETIEADGLLERGAAIGARFREQFEALRQELPSL
                     IRDIRIMGTMIGLDLTIDATETVTACLGRRLLINATHGHVVRLLPALTISDDQIDEGC
                     AIMADVLRQAVV"
     gene            2238..>2562
                     /locus_tag="VHS97_05245"
     CDS             2238..>2562
                     /locus_tag="VHS97_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX3447634.1"
                     /translation="MPRHFLDLLEISAAEARWLIDQSIKIKRDEKEGRRPPLLAGRTL
                     GLLFEKPSLRTRVSFEAAIARLGGNAIFLHGKDVGLGVRESLVDFARVLSQYVDALAA
                     RTFSHS"
BASE COUNT          497 a          789 c          798 g          478 t
ORIGIN      
        1 cgggaagttc acggtgatca agcttggcgg ctcggtcatg gaagatcccg agtcgctgcg
       61 cgctctcctg gtcgacgtcg tcttcatgca gacggtggga atgaggccgg tcatcgtgca
      121 cggcggcggc aaggcgatca ctgtggcaat ggaaaaagcc ggactgaccg ctcgcttcgt
      181 caagggaaga cgctttaccg acgaaccgac gctcgcgatc gtcgcccgcg ttctggccga
      241 agagatcaac gccgacatcg agcgccacat caacaagttt gggggccgcg cctcggggtt
      301 acaccacaaa acacaccagt gcttgtacgg ccgccggttg tggctggata acggaccgga
      361 agggccgatc gatctcggcc gtgtcggcga ggtcaccgcg gttgacgtgc cgcccatcga
      421 aaacctctgc ttggccggtg tcgtgcccgt gctcccctca ctcgccgagg accagcagga
      481 tgaaggccgg ctactaaacg tcaatgccga caccgcggcc gcggccgtgg cccaggccct
      541 tcaggccgaa aaactcgtgt ttttgaccga caccacggga atcctcctcg acaaaaatga
      601 acccgcgacc ttgatacggg gtctgacccc gagcggttgc cacgatttga tcgcccaggg
      661 agtgatcgac cgcggcatga ttcccaaggt cgaagcctgc ctgtccagtc tggaagcggg
      721 ggtcttgaaa acacacatca tcgacggccg gcttcctcat gcattgcttc tcgaaatatt
      781 caccaagagc ggtatcggca ccgagatcgt ccgcgacgaa aatagcccct cgcccaccgg
      841 acaccggccg gtactcggca gaccgtgaca cagtcgatag tcgatcgacc tggaaccggg
      901 caactccaag ttacagaagc acaatctcat atttagctat catttataat ttatcaacgt
      961 tccgcgaggc attgattcgc acgcgaatac atgcggggag acacagcttt gtcgacaacc
     1021 caacttgccc atcaaagttc tcaggcgacg atcgcgcagt tcgagcgcta cgtcattgcc
     1081 aactatcggc gctaccctgt cagcctggtc aggggcgaag ggtcgtggat ctgggacgca
     1141 gagggacacc gttatctcga cttcttcccc gggtggggtt gcaacctgtt aggccactgt
     1201 ccgccgcgcg tcgttgaggc cgtccgcgac caggtcggcc agttgatcca cgtaccgaat
     1261 acctggtaca tggaggcaca aggagcgctg gcccaggccc tatccgagcg gtcgttcggt
     1321 ggtcagtgtt tcttctgcaa cagcggcgcc gaggccaatg aggctgcgat taagctggcg
     1381 cgggcggtcg gtcatcccag tgggcgcacg aagatcgtca ccatggaagg gggtttccac
     1441 ggccggacct acgccgccct tacagccacg gcgcagccca agtaccacat cggcttcgag
     1501 ccgatggttc ctggtttcgt ctacgtcccg tataacgata tcgcggcggt cgcggcggtg
     1561 ctcgacgatc agacagctgc ggtcatggtc gagccgatcc agggggaggg gggcatcaat
     1621 atcccgtcgg cgacttatct cggcgagctg cgacggctgt gcgacgacca cggggtgctc
     1681 ttgatcctgg acgaagtcca gaccggcatg ggccgaacgg gtgagtggtt tgcctatcag
     1741 catggcggag tcgtgcccga catcctgacc tgcgccaagg ctttggcggg gggggttgcg
     1801 gccggcgtga tgatcgcccg gcctgcggtc gccgccgtgc tcaagcccgg gatgcacgcg
     1861 tcgaccttcg gcggcaaccc gatcgcctgc cgcgcggccc tggccgccat cgaaaccatc
     1921 gaggccgacg gtctgctcga gcgcggggca gctatcggag cgcggttccg cgagcagttt
     1981 gaggccctgc gtcaggagct gccaagtctg atccgagaca tccggatcat gggcacgatg
     2041 atcggtctcg atctgaccat tgatgccacc gaaactgtca ccgcgtgcct gggccggcgg
     2101 ctcttgataa atgccacgca cggccatgtc gtccggctct tgcccgcatt gacgatcagc
     2161 gacgatcaga tcgacgaggg atgcgccatc atggcggacg tattgcggca ggcagtcgtt
     2221 tgaaacggat ctcggaaatg ccacgtcatt ttttggactt gctggaaatt tcggcggccg
     2281 aggcgcgctg gctgatcgat caatcgatca agatcaagcg cgacgaaaaa gaggggaggc
     2341 gtccgccctt actcgcaggc cggacgctcg ggctcctctt cgagaaacct tcactccgaa
     2401 cccgagtcag cttcgaggcg gcgattgctc ggctgggtgg caacgcaatc tttctgcacg
     2461 ggaaggacgt agggctaggg gtacgcgaaa gcctcgtcga cttcgcccgc gtcttgagtc
     2521 agtatgtcga cgccctggcc gcccgcacct tttcgcactc ca
//
LOCUS       DATAQL010000305         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1710235, whole genome shotgun sequence.
ACCESSION   DATAQL010000305 DATAQL010000000
VERSION     DATAQL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710235"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1207)
                     /locus_tag="VHS97_05250"
     CDS             complement(275..1207)
                     /locus_tag="VHS97_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX3447635.1"
                     /translation="MPAFTRRVGEFVHRHSPPLDGACDAWGKRSAFAAARLVSIVAAV
                     CSAIPALAGSDEENLANQTGDDPTHPLGKIELLDRFTTAPGPGAAEGTTKQVDTNTPF
                     ARIETPFRIAPQWELGFRVEIPVVWTNPVTPQNPSGQVITGFGNVLTQSWLIYEINPR
                     WAVAAGAQVIAPTATNGVASDAWEEVPGAVVRVMLPELSEGSYFAPQVRYGVDFGGND
                     EGPLLRQLRLMPALNINFPHDLFLTFYPSPDIRWNYGTPVHGQTGRFFLPLNFMIGWK
                     PTEHTVISAEFGIPIIKEFPAYTFKTQVRIGYLF"
     gene            complement(1410..>1599)
                     /locus_tag="VHS97_05255"
     CDS             complement(1410..>1599)
                     /locus_tag="VHS97_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447636.1"
                     /translation="NERDPIIGNQDGTLEFKIPKRPIRKKIVGLPAFTTVRGGAYFFL
                     PGLGALGYLASLKEAGGA"
BASE COUNT          303 a          476 c          499 g          321 t
ORIGIN      
        1 tcgcggatca gccgggctgc atgggccaga gtccgcaccg gaaccggtcc gtcattggct
       61 gccatgccgc cgaactcggc gcgggtcacc agcactgtgt gtgcgcccaa gtcggcagcg
      121 cggatggcgg cgagtacacc tgccggtcca gcgccaataa cgatgacgtc tatcatccct
      181 ggcttcccga cgataaacga gacctgttcc ccttcagcgg acgccatcca atgtcgcaag
      241 gtatcgaagg gcattgaatc atgaagacct agaatcaaaa caagtagccg atcctgactt
      301 gagtcttgaa tgtatacgcg ggaaactctt taatgatcgg gatgccgaat tccgccgata
      361 ttaccgtatg ctcagtgggc ttccagccga tcatgaagtt gagaggcaag aagaaccggc
      421 cggtctggcc atgtaccggg gttccgtaat tccagcgaat atctggactc ggatagaagg
      481 tcagaaacag atcgtgaggg aaattgatgt tgagggccgg catgaggcgg agctgccgca
      541 acaagggtcc ttcgtcgttg ccgccaaaat cgacgccgta gcggacctgg ggcgcgaagt
      601 agctgccctc gctcagttcc gggagcatca cgcggaccac cgcgccgggg acctcttccc
      661 atgcgtcgga cgcaactccg ttggtcgcgg tcggggcgat gacctgcgcg ccggccgcga
      721 ccgcccagcg cgggttgatc tcgtaaatga gccagctttg ggtgagcacg ttgccgaagc
      781 ccgtgatcac ctgtccgctg ggattttgcg gcgtcactgg atttgtccag acgacgggga
      841 tctcgaccct gaaacccagt tcccactgcg gcgcgattcg gaacggggtc tcgatccgcg
      901 cgaacggcgt attggtgtcg acctgctttg tcgtcccctc ggccgcgccc ggccccggcg
      961 ccgttgtgaa gcgatcgagc agctcgatct ttcccagagg gtgcgtcgga tcgtcgccgg
     1021 tctggttcgc caaattctcc tcgtccgagc cggcgagagc cggaattgcc gagcaaacgg
     1081 ctgccacaat cgagactaga cgtgcggcgg caaaggctga tcgtttgccc cacgcgtcgc
     1141 aggcgccgtc aagtggtgga gagtgtcgat ggacgaattc gccgacgcgc cgagtgaagg
     1201 cgggcatcgc gcggccactc cgttcatcga atcgtggcaa agtttctccc gaagttctcc
     1261 agactctcag tgccgccgaa aagctggtga aattggtcga gtcgaccacc acgtccagta
     1321 gatgctcacg cggcgtttgc ctggcttgta tttgcggcgg aagttggtgc tgtcatggct
     1381 atcgccttcc gatgactcct gctcgcggcc taggctccgc ccgcctcctt tagcgacgcg
     1441 aggtatccaa gcgctcctag gcccgggaga aaaaagtacg cgccgcctct gaccgtggtg
     1501 aaggccggca aaccgacaat tttcttgcgg atcggacgct tggggatttt gaattccagc
     1561 gtgccgtctt ggtttccgat gatcgggtcg cgttcgttg
//
LOCUS       DATAQL010000306         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4303972, whole genome shotgun sequence.
ACCESSION   DATAQL010000306 DATAQL010000000
VERSION     DATAQL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4303972"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="VHS97_05260"
     CDS             complement(<1..1518)
                     /locus_tag="VHS97_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX3447637.1"
                     /translation="MRPRRDRSRIPDKGQTRKRGKTAGSGRSTVEPGQLIVLSERDDS
                     QTLQPCRIPLLPLRSDVVFPQTVVPLVINRPSGIRLIDDCMIGERMVGLVSQLHPETD
                     SPGMNDLFPMICVGLILKMLKFPDGSTRIVCQGQYRARLVAVEQTEPYLMGRIEPREE
                     VVEAGVELDARVHHVNRFFQRMVDQSQQIPEELQVAAINTREPGRLADLLASSLPFTI
                     EEKQSLLGEINVRARLERLGQYLSRQLAILELSTKIQEQVGSELSKAQRDHFLRQQMK
                     AIQDELGESESENPEIGELWDRIKNANPPKEVQSEAERELERLAGMHPSSAEYSIVRT
                     YLDWLAILPWSKASRDRLDLRRARKVLDTDHFDLEKIKERILEYLAVRKLKKDMKGPI
                     LCFAGPPGTGKTSLGKSIAKALGREFVRISLGGVHDEAEIRGHRRTYVAALPGRVIQG
                     IRKANTNNPVFMLDEVDKLGADFRGDPSAALLEVLDPEQNSTFRDHYLDVDFDLSK"
     gene            complement(1670..2476)
                     /locus_tag="VHS97_05265"
     CDS             complement(1670..2476)
                     /locus_tag="VHS97_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3447638.1"
                     /translation="MLGASETPYDLRFSLLGIPVRVHPLFWLVAVVLGFGRNDRNLAL
                     VVVWVACVFVSILVHEYGHALMARAFGSSPSIVLWGMGGLCYSQAERQSLLQRLAVVL
                     SGPAAGLGLYVLVMLFASGTFGISASENLSVSQATLPGLGMLVSADDWFSAQNKLHNE
                     VYFESFLYLIQINLLWSLFNLLPIWPLDGGQATQILLSLYDRARGPRWGHIVSLLCAG
                     GLAIMAVSMGASLFNALFLAYFAMMNYQMLQTLHQAHSMGIYHEDEWWKR"
     gene            2941..3777
                     /gene="dapF"
                     /locus_tag="VHS97_05270"
     CDS             2941..3777
                     /gene="dapF"
                     /locus_tag="VHS97_05270"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX3447639.1"
                     /translation="MRFTKMHGIGNDYVFMSTFDQAPPADPARLATVLSDRHCGIGGD
                     GLILIMPSERADARMRMFNADGSEGEMCGNGVRCVAKYLFDHGLARHQQVAIETGRGI
                     LTLQLEVSGGRVDKVRVDMGPPILEATQIPTHLPGNPPVSVSVQVGVRELAVTAISMG
                     NPHAVVYLPDVDDFPLESVGPILERHPAFPKRVNVHIVQVLDRNEVRMRTWERGSGIT
                     LACGTGACAVCVAGVLTGETARAILAHLPGGDLELSWPDDRSSVFMTGPATEVFSGEW
                     PS"
     gene            3904..>4196
                     /locus_tag="VHS97_05275"
     CDS             3904..>4196
                     /locus_tag="VHS97_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447640.1"
                     /translation="MTEPDATSEDSCLTEPADEEFAPTLDSAAALASILDRYMADLQA
                     GRVPDRQQLCDAHPELATQLEACVAGLEFIHRATGPVTETPTTLGEFRIIRELG"
BASE COUNT          854 a         1284 c         1234 g          824 t
ORIGIN      
        1 cttggacagg tcgaaatcga cgtccaggta gtggtcgcga aaggtggagt tttgttcggg
       61 gtcgagaact tccaggaggg cggccgaggg atcgccgcgg aagtcggccc ctagcttgtc
      121 gacctcgtcg agcatgaaaa ctgggttatt ggtgttcgcc ttgcgtatac cctggattac
      181 ccgcccaggg agcgcggcga cataagtccg gcgatgaccg cggatttcgg cctcgtcatg
      241 gacgccgccc aagctaattc gcacgaattc gcgccccaag gccttagcga tgctcttgcc
      301 cagcgaggtc ttacccgtcc ccgggggacc cgcgaagcag aggatcgggc ctttcatgtc
      361 ttttttcagc ttgcggaccg ccaggtattc aagaatccgt tccttgatct tttccaggtc
      421 gaaatggtcc gtgtcgagga ccttgcgcgc tcgcctcagg tcgagccggt cgcggctggc
      481 cttgctccag gggagaatag ctagccagtc gagataggtc cgtacaatcg aatattccgc
      541 cgagctgggg tgcataccgg cgagccgctc gagctcgcgt tccgcctccg actgcacctc
      601 tttcggtggg ttggcattct tgatcctgtc ccacagctcg ccgatctcag ggttttcgct
      661 ctcgctttcg cccagctcgt cctgaatcgc tttcatctgc tgccgcagaa aatggtcgcg
      721 ctgggccttg gagagctcgc tccccacttg ttcttgaatc ttggtcgaga gctcgaggat
      781 cgcgagttgc cgcgatagat actgacccaa ccgctccaac cgggctcgca cattgatctc
      841 gcccaagagg ctttgcttct cttcgatcgt gaacggcagg ctcgaggcca gcagatcggc
      901 cagccggcct ggttcgcggg tattaatcgc cgcgacctgg agctcctcgg ggatctgctg
      961 gctctggtcg accatgcgct gaaaaaaccg attcacgtga tgaacgcggg cgtcgagctc
     1021 gaccccggcc tcgacgactt cctcgcgtgg ctcgattcgc cccatcaggt aaggctcggt
     1081 ttgttccacc gcgaccagcc gggcgcggta ttgcccctgg cagacgattc gcgtcgaacc
     1141 gtcggggaac ttgagcatct tcagaatcaa accaacgcag atcattggaa agagatcgtt
     1201 catcccggga gaatcggtct cgggatgaag ctggctcacc agaccaacca tccgttcacc
     1261 aatcatgcaa tcatcgatca accggattcc actgggacgg ttgatcacca gcggaacgac
     1321 ggtttgggga aagacgacat cggaccgcaa gggaagcaaa ggaattcggc acggctgcaa
     1381 ggtttggctg tcgtcccgct cggagagaac gatgagctga cccggctcga ccgttgaccg
     1441 gccggagccg gcggtttttc cccgcttccg cgtttggccc ttgtccggaa tccttgatcg
     1501 gtctcttcgt ggacgcatgg atatcttttc tgagatgact ctctattgcg aaaaactgcg
     1561 acccgtagcc aatcctagga tcggaacgcg catggattcg cgtggaaggt taataattgt
     1621 ggattatcgc cgcgaaagga agaggtgacc tcagttaagt aaggtcgctt tagcgcttcc
     1681 accactcgtc ttcgtgatag atacccatcg aatgggcctg gtggagcgtt tggagcattt
     1741 gataattcat catggcgaaa taggcgagaa aaagggcatt aaagaggcta gcccccatcg
     1801 acacggccat gatagccagt ccgccggcac acagcaatga aacgatgtgg ccccatcgag
     1861 gaccccgcgc gcggtcataa agcgagagca ggatctgagt cgcttggccg ccgtccaacg
     1921 gccagattgg caaaaggttg aacaggctcc agagaagatt gatttgaatc agataaagaa
     1981 aggattcaaa gtagacttcg ttgtgcagct tgttttgcgc cgaaaaccag tcgtcagcgc
     2041 tcacaagcat gccgaggccg ggcagggtag cctggctcac gctcaggttt tcactggccg
     2101 aaatgccaaa cgtgcctgag gcgaaaagca tgacgaggac atacagcccg agccccgctg
     2161 cgggaccgct cagaacgacg gccaaccgct gcaagagcga ctgccgctcg gcctggctgt
     2221 agcagagccc gcccatgccc cacaacacaa tcgatggcga gctcccaaag gctctcgcca
     2281 tcagtgcgtg cccatactcg tgaaccaaga tcgagacgaa cacgcacgcc acccacacga
     2341 ccaccaaagc caggttgcga tcattcctac cgaacccgag cacgacggcc accagccaga
     2401 acagaggatg aacgcgtacc gggatgccca gcaagctgaa ccggagatcg tagggcgttt
     2461 ctgaggctcc aagcatcggt gacctcgctg gaggtgacgg tatgtaaaac gaaagtcggc
     2521 agacgccctt attctatggt ggaggagact catccgcaaa ccagacccgc acgcgtcttc
     2581 gtcctggacc ccgtggccgc ccggccttga cgtgcgactc caacataaaa aagtccgctg
     2641 tcatcggaac ggttcatcga cttgtgacga ctacccgtcg ctgttattgt cgaactgagc
     2701 gccaagacgg gtgtccgatc gatgatacgt cgaataaagt ggccgatatc tggcatcgtt
     2761 caactttagc atcttcgcag gtaacaagag ctgggtggct ggggtcgagc tggcgacagc
     2821 tagcgagccc ccggcgcgga agcctcggat ctgggggcgt cgccctgcgg gcgtcgaccc
     2881 cagccacccc ctgaggtgta acctgctttt agaatctccc cgcatgactg gaacaggact
     2941 ttgcgtttta ccaagatgca cggaatcggc aacgactacg tcttcatgag caccttcgac
     3001 caagctccgc cagccgatcc cgcccggctc gccacggtgc tcagcgatcg ccactgcggc
     3061 atcggcggtg acggcctgat actcatcatg ccgtccgagc gtgccgacgc ccggatgcgc
     3121 atgttcaatg ccgatggctc cgaaggagag atgtgcggca acggcgttcg ctgcgtcgcc
     3181 aagtatcttt ttgatcacgg cctggcacgc catcagcaag tcgcgattga aaccggccga
     3241 ggaattctca cgctccagct cgaggtatcg ggagggcgag tcgacaaggt tcgcgtcgac
     3301 atgggcccgc cgatcttgga agcgacgcag attcccactc acctacccgg caacccgccg
     3361 gtctcagttt cggtccaggt cggcgtgcgc gagctcgcgg tcacggctat ttcgatgggg
     3421 aaccctcacg cggtcgtgta cctgccggac gtcgacgact tcccattgga atcagttggc
     3481 ccgatcctcg agcgacatcc cgcatttccc aagcgcgtca atgtgcacat cgttcaggtt
     3541 ctcgatcgga acgaggtgcg gatgcgcacc tgggagcgag gttcagggat cacactggcc
     3601 tgtggcacgg gtgcctgcgc cgtctgcgtc gccggagttc tcaccggaga gaccgcccgc
     3661 gcaatcttgg ctcacttgcc tggtggcgat ctcgaactgt cctggcccga tgaccggtcc
     3721 tcggtcttca tgacagggcc ggcaaccgag gtcttctcgg gcgaatggcc gagttgaatg
     3781 cggcgatttc attttctctt atccgccgcg tgacattcgc gaagttctcg gccctatcat
     3841 ggaggggaag gctcgtccac gtcctttccc cgtcaaccaa tgacagcgcg ggagcgaccc
     3901 cagatgaccg agccggacgc cacgtcggaa gactcatgtc tcaccgagcc cgccgatgag
     3961 gaattcgctc cgacgctcga ctcagccgcg gcgctggcga gcatcctcga ccgctacatg
     4021 gccgatctcc aggcgggccg tgtgcccgat cgccagcagc tttgcgacgc gcatccggag
     4081 ctggccacac agctcgaggc gtgcgtggca ggcctcgaat tcatccatcg agcgaccggc
     4141 cccgtaacgg aaacaccaac gactctgggc gagttccgga tcatccgcga gctcgg
//
LOCUS       DATAQL010000307         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5221054, whole genome shotgun sequence.
ACCESSION   DATAQL010000307 DATAQL010000000
VERSION     DATAQL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5221054"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..>2195)
                     /locus_tag="VHS97_05280"
     CDS             complement(114..>2195)
                     /locus_tag="VHS97_05280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3447641.1"
                     /translation="FKARHRVLKRIVALKLLPPSLARDPSAVQRFHREAKAAATLSHP
                     NVVAVLDADEFRGLHFLVMEYVEGRDLARLVRQQGPMSLTQAIDCIIQAARGLKAAFE
                     ASVVHRDIKPSNLLLEPGGTLKILDMGLALLDAVGGPFGANQPDASLTQSNILLGTID
                     YMSPEQATNPRNADHRSDIYSLGCTLHYLLTGRPPYSGETLIERLVAHREQPVPRLSD
                     SVPDVSPALDVLLRRVLAKSPTDRFSTFDELIAGLEACRPGAVTDINEWPALQIVTDP
                     PEPTGRRRAPIAAAIACVAAAAGILVMGIYLSRRQSVPSDQVPPLVSDTPVFAKNGSV
                     PQPAPRVTDPQRPALAAGERQPEPKPIATAASSRSAVRAKASLPAHAAGLVREFRGHG
                     GRVNGIAVSGDGLLALSGGQDRTVRLWDVATGNELLRVDHDGPVDAVAITADGRHGLS
                     GSDDKTVRLWDFRPANTVGMRRLDGHTRVVFAVAFALGDQLAVSGGADRTVRAWDLAT
                     GQPSGFPLLHESAVAALATIGDDTVLAGCDDGTLWLWDLRSCQRIRRLKAPGPVLCVA
                     GSPLGHRALSGHADGVLVLWDLDLGAEIGRMVGHNDLVRSAALLPDGRRALAGSQFGN
                     LSLWDLDTRREIRRFSPAATTSDHAGQLGVAILADDVHALTADTDAIVRLWSLPASDA
                     STDAAGRRTAP"
BASE COUNT          399 a          702 c          736 g          358 t
ORIGIN      
        1 gccgattctg cacccgaaca aggcgtcgac ggcaaagtcc gcggtttcgt ccaagagcaa
       61 aaagacggct cccgctcccg cgacggggcg atcgcctgcg acttgatgca ccatcagggt
      121 gcagttcgcc ggcccgcagc gtcggtcgac gcatcgcttg cgggcaggct ccagagacga
      181 acgatcgcgt cggtgtcggc ggtcagagcg tgaacatcgt cggcgagaat ggcgacgccg
      241 agctggcccg cgtggtcgga ggtggtggcg gccggagaaa atcggcggat ctcgcgtcgc
      301 gtgtcgagat cccagaggct cagattgccg aattggctgc cggccagcgc ccgacggccg
      361 tcgggcagga gggccgcgga gcggaccaag tcgttgtgac ccaccatgcg gccaatctcg
      421 gcacccagat cgagatccca aagaaccagg acgccgtcgg catgcccgga caacgctcga
      481 tggcctaggg gtgagccggc gacgcataag accgggccag gcgccttcag tcgtcggatc
      541 ctctggcagg acctgaggtc ccacagccac agcgtcccat cgtcacaacc cgccaggaca
      601 gtgtcgtcgc cgatcgtcgc cagtgcagcg accgcgcttt catgtaagag cgggaaaccg
      661 gaaggttgcc ccgtcgcgag atcccaggcg cggacggtcc tatcagcgcc tcccgagacc
      721 gcgagctgat ctcccagggc aaacgcgacg gcgaacacca cccttgtatg cccatcgagc
      781 cggcgcatgc cgactgtgtt tgccggtcga aaatcccaaa gcctgaccgt cttgtcatca
      841 gagcccgaaa gcccgtgccg gccgtcggca gtgatcgcca cggcatccac cgggccatcg
      901 tgatcgaccc gcagaagttc atttccggta gcgacatccc acaggcggac ggtccggtcc
      961 tggcctccgg agagagccag caggccgtca cctgaaacgg cgatcccgtt cacgcgcccg
     1021 ccatgccccc tgaattcgcg cacgaggccc gcggcatggg ctggtaggct cgctttcgcg
     1081 cgaactgcac tcctcgacga cgcggcggtg gcgattggtt tcggttccgg ctgccgctcg
     1141 cccgcggcta gcgccgggcg ttgcggatca gtcaccctgg gtgccggctg gggtacactc
     1201 ccattcttgg cgaagaccgg tgtgtccgaa accagcggag gcacctggtc gctcggcacg
     1261 gattgtcggc ggctcaagta gatccccatc acgagtattc ctgccgccgc cgcgacacac
     1321 gcgatggcgg cggcgatcgg cgcacgccgt cgtccggtcg gttccggcgg atcggtcacg
     1381 atttgtaggg cgggccattc gttgatatcc gtcacggcac ccggacggca cgcctccagg
     1441 ccggcaatca gctcgtcgaa cgtcgagaat cgatcagtcg gagatttcgc caggacgcgc
     1501 cgcaggagaa cgtccaaggc gggcgatacg tcgggcacgc tgtccgaaag ccgcgggacg
     1561 ggttgttcgc ggtgcgcaac gagccgttcg atcaaggttt cacccgaata cggcggccgg
     1621 cccgtcaaca agtagtggag cgtgcaaccg aggctgtaaa tgtccgagcg atggtccgcg
     1681 tttctcgggt tggttgcctg ttcgggcgac atgtagtcga tcgttcccag caagatattg
     1741 ctttgggtca gactggcgtc cggctggttt gcgccgaagg ggcccccgac cgcgtcgagg
     1801 agagccagtc ccatgtcgag gatcttgagc gtcccaccgg gctcgagcag gaggttggac
     1861 ggtttgatgt cgcgatggac gaccgaggct tcgaaagccg ccttcagccc gcgggccgcc
     1921 tggatgatgc agtcgatggc ttgggtaagg gacatgggcc cttgctgacg aaccaatctc
     1981 gcgaggtcgc gcccttcgac atattccatt acgagaaagt gcagcccgcg aaactcgtcg
     2041 gcatcgagca cagccacaac gttgggatgg gagagcgtgg ccgcggcctt cgcctcgcgg
     2101 tgaaagcgct gtacggccga ggggtctcgg gccagggaag gcggcagcaa tttaagggcc
     2161 acaatgcgct tcaagacgcg atggcgcgcc ttgaa
//
LOCUS       DATAQL010000308         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3237146, whole genome shotgun sequence.
ACCESSION   DATAQL010000308 DATAQL010000000
VERSION     DATAQL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237146"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VHS97_05285"
     CDS             complement(<1..902)
                     /locus_tag="VHS97_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX3447642.1"
                     /translation="MTGDDKLARLRPRSPFVLIGLWFCIYVYAHSPSRAEPASGSGDN
                     QTKPDPAGVEYFEKSVRPILATRCQGCHGPAKQKGGLRLDARTALLVGGSTGPALVPG
                     NPAESLLVDAINYGEALQMPPKSKLPDAEIATLTEWIKRGAPLGAQMPIDPANEANAK
                     PSGKSDQLSRDEYQKRARFWSFQPIKHVTPPALKTGPLDWARNPIDQFIQSAMTEHGL
                     APSPEADRRTLIRRLSFDLLGLPPSPIEVAAFVADAGADAYERLVDRLLASPHYGERC
                     ARHWLDLVRYAETGGHEFDYDIVNA"
     gene            1311..>2435
                     /locus_tag="VHS97_05290"
     CDS             1311..>2435
                     /locus_tag="VHS97_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3447643.1"
                     /translation="MSVSNPHRLTGYLMAAAGVVLAGLSPWWLTPFTGDSPPMRLMLV
                     VVVGLSAWLSGFGPALFATILGLVAIVLANDAPGDWPTVWSRMWRFGSLALLISLLFR
                     GLNQSRRQAQAKEEKFRCSEARYRRLIETSGQGIWVIDQEERTTYANPRLGEILGISP
                     ARLIGMRLHEILVDDAASWTGADTQPDPFAWHEVRLHGGDGRVRHAIVTSRAIGPDEV
                     PADATPSHGITAGGLLIMVTDVTPLKETEEALRDKESVLRSFYESSVMAMGVVQLTEN
                     DSRFVSANALTDKFFGVAPGALEGMSARELEAPPEMLTTWNERFRECQATGRPVRFEY
                     RGTCESSPAWVSATLSPMDSRGTGRALCSFIVEDITDRKRT"
BASE COUNT          456 a          694 c          798 g          487 t
ORIGIN      
        1 gcattgacga tgtcgtaatc aaactcgtgg ccaccggtct cggcgtagcg aaccaggtcg
       61 agccagtggc gggcacatcg ttcgccgtaa tgggggctgg ccaggaggcg atcgacgagc
      121 cgctcgtaag catcggcgcc agcatccgca acgaaggctg cgacttcgat cggactcgga
      181 gggagcccca aaaggtcaaa gctcagccga cgaatcaagg tccgccggtc ggcctccggc
      241 gacggtgcca gcccgtgctc ggtcatcgca gactggatga actgatcgat cggatttcgt
      301 gcccagtcaa gggggccggt cttcaaggcg ggtggcgtca cgtgctttat gggctggaac
      361 gaccagaatc gagcccgctt ttggtattca tccctgctta gctggtcgga cttaccacta
      421 ggtttggcgt tggcttcgtt ggcgggatca atcggcatct gggctcccaa gggagcgcct
      481 ctcttgatcc actcggttag tgtggcgatc tcagcgtcgg ggagtttcga tttgggaggc
      541 atctggagtg cttcgccgta gttgatggcg tcgacgagca ggctctccgc tgggttgccg
      601 ggcacgagag ccggaccggt cgatccgcca accaggagcg ccgtacgcgc atcgagccgg
      661 agcccaccct tctgcttggc gggtccatga cagccctgac aacgagttgc caggatcggc
      721 ctcacactct tctcgaaata ctctaccccg gccgggtcgg gtttggtctg gttatcgccg
      781 ctcccagaag cgggctcggc gcggcttggt gagtgtgcgt atacataaat gcaaaaccat
      841 agaccgatca agacgaacgg gctgcgcggg cgcaggcgtg cgagcttatc gtcgcctgtc
      901 atctgtcggt gccccctttt gccgtcacga gccggatcaa cttcaagcaa ttgtttaagt
      961 ttacaaggac aagccgatcc ggacgaggct ttaccgcaac ggtgttccgc gcgtcgctac
     1021 gcagtgcacg accgttagcg gcgtaaaatg gcattaaaca ggttataata acgcgcgcag
     1081 accggctctg gcggttgacg acaatcagtg aagcgatgtt gagatagaac ctcacgacag
     1141 ccgaaaggcc agcatataaa ggattgagat gtcggacaac tcgctcttgg gagatgggtc
     1201 caactgttgg taagatgacc agagccgcta cccaggccgg tcagaatcgg ggaaaaaggc
     1261 cggtcattat tgagatcgaa tgcctgggat tggggtcgaa gggagtgagc atgtccgtgt
     1321 cgaaccctca ccgcctgacg ggctatctca tggccgcggc gggtgtggtt ttggcgggtc
     1381 tgtcgccctg gtggttgact ccattcaccg gcgactcccc gccgatgcgg ctgatgctcg
     1441 tggtcgtggt ggggctttct gcgtggctca gtgggtttgg cccggccttg ttcgccacga
     1501 ttctggggct cgtggcgatc gtccttgcga atgatgcgcc cggtgattgg ccaaccgtgt
     1561 ggagtcggat gtggcgtttt ggctctttgg cgcttttgat cagcttgctg ttcagggggc
     1621 tgaatcaatc tcggcggcag gctcaagcca aggaggaaaa gttccgctgc agcgaggcac
     1681 gctaccgccg cttgatcgaa acctccggac agggaatttg ggtgatcgat caggaagaac
     1741 gaaccacgta tgcgaatccc cggttgggag aaatcttggg gatatcgccg gcgcggctca
     1801 tcggcatgcg cctccatgag atcctggtcg acgacgccgc gtcttggacc ggcgcggata
     1861 ctcaaccaga ccctttcgcc tggcacgaag tccggctaca tggcggtgac gggcgcgtgc
     1921 gtcacgcgat cgtgacgtcg cgcgcgatcg gacccgacga ggtgccggcc gacgcgacgc
     1981 ccagccacgg aatcacggct ggcggcctgc taatcatggt caccgacgtc acgccgctca
     2041 aagaaaccga ggaggcgctc cgagacaaag agtcggtgct tcggagcttt tacgagtcat
     2101 cggtgatggc catgggcgtt gtccagctca ccgagaatga ttcgcggttc gtttcggcca
     2161 atgcgctcac cgacaagttt ttcggcgtgg cgcccggtgc gctcgaaggt atgtcggctc
     2221 gtgagctaga ggcgccgcct gagatgctga cgacctggaa cgagcgattc cgggagtgcc
     2281 aggcgacagg ccggccggtc cgattcgagt accgcggcac gtgcgagagt agcccggcgt
     2341 gggtctcggc cacgctctcg ccaatggact cgcgcggcac cggccgtgca ctgtgttcgt
     2401 tcatcgttga agacatcacc gaccgcaagc gaacc
//
LOCUS       DATAQL010000309         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4762424, whole genome shotgun sequence.
ACCESSION   DATAQL010000309 DATAQL010000000
VERSION     DATAQL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4762424"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VHS97_05295"
     CDS             complement(<1..643)
                     /locus_tag="VHS97_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447644.1"
                     /translation="MESSNEPPAKGRGGFVGGLGELDRILRGELTQVSSLRRGGIAVS
                     PGRLSLIIVVLGMVFGVCMGTFALFRVNKPHAWQIVASMVKVPLLFYLTLLVTLPSLY
                     VFNALVGSRLTLATVVRLLIASLGVMVAVLASLGPIVAFFSVSTTSYLFMVLFNVVVC
                     GVSGGLGLLFLMQTLHRLSVLDSRPPAAQNTEGGDVTEPVGPLDPLENRVLGKH"
     gene            885..2756
                     /locus_tag="VHS97_05300"
     CDS             885..2756
                     /locus_tag="VHS97_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3447645.1"
                     /translation="MSLIDYLLGRRLATYEEGEQRVGVIAGVPMLGLDALGSAAYGPE
                     AALTLLIPLGAAGVSYIGPISTLIILLLMVVYFSYRQTIAAYPHGGGSYTVAKDNLGT
                     TPGLFAAAALLLDYVLVVAVGISAGVGALVSALPVLQPHTLSLCLAILLFITLVNLRG
                     VRESGLAFMAPTYLFIVTLLAVIAIGIFKTVLAGGHPVPVVAPPPLPSATTAASLWIL
                     MRAFASGCTAMTGVEAVSNGVTAFREPAVPHAQRTLTVIIGLLAVMLAGIAFLCRAYG
                     IGATEPGQPGYESTLSQLTAAVVGRGLFYYITIGSVLSVLALSANTGFADFPRLCRVI
                     AADGFLPSGFAHRGRRLVYSQGIIVLAVLSAGLLIVFGGITDHLIPLFAIGAFLAFTL
                     SQAGMVVHWLRLGGAHAKKSMVINAAGALSTGVTLGVVVVSKFLEGAWVMMLLVPGLL
                     GLFYTVRTHYQRVGREVASDTPLDAEGLTPPVVLLPIRGWSAITRKALRFALKISDEI
                     YVLHIAGDEQTMVRLEEGWVRLVREPATKAGLQPPKLIIIYSPFRQMYKPLIEVVSDL
                     KRAHPDRDIAVIIPELIGTRWYHYVLHNQTAAVMVAYLRLSGFRKVAVINVPWYLSD"
     gene            complement(2753..>3693)
                     /locus_tag="VHS97_05305"
     CDS             complement(2753..>3693)
                     /locus_tag="VHS97_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX3447646.1"
                     /translation="ILTAQGVDPAGIEPDLPLSRPRLLNFSTFRRKVNPVFYQAGEDQ
                     HACANCHGNHTILRIAVADASGGFSGEQLMINYNSALKVVNLGDPEASLILRKPRSPQ
                     GQGEAEPSSDTGLSHVGGPRWENTEHPAYRAILEWVRDASRSATLDAGNEKYSSDSYS
                     PGYEPSRAGDGDLTTIWRTEFVGATPGYPHELVVELSGPRQVEGLLYVPRQDSPDGRV
                     KEFEIRVSDDGKTWGMPVAKGRWSNDPSFKYVALPGPRGRYVQLRGLSEVEGRPFMSA
                     AELSIDTSPLAVDERAHGRTPSSPISAINPPSPLPH"
BASE COUNT          673 a         1096 c         1134 g          790 t
ORIGIN      
        1 catgcttgcc cagaacgcgg ttctcgagcg gatcgagcgg cccgaccggc tccgtcacat
       61 ccccgccctc ggtgttttgc gccgccggcg gtcgcgaatc gagtacgctt aaccggtgta
      121 atgtttgcat cagaaacaac agcccaagac cacccgatac gccgcagacg accacgttga
      181 agagcaccat gaacagatag ctggtagtgc tgaccgagaa aaatgccacg atcggcccaa
      241 gcgacgccag cacggctacc atcactccca gcgaagcgat caacaaacgc acgaccgtcg
      301 ccaatgtcag ccgcgaccca accagggcat tgaatacata aagagacggc aacgtgacca
      361 gaagtgtgag ataaaacaag agcggaacct tgaccatgct cgcgacgatc tgccaggcgt
      421 gtggcttgtt cactcgaaaa agggcaaacg tccccatgca gacgccgaag accataccca
      481 gtacgacaat gatcagcgac agccgcccgg gggaaacggc aatccccccg cgccgtagtg
      541 aagagacttg cgtcagctca ccgcggagaa ttcggtcgag ctcgccgagc ccgcccacga
      601 atccacctcg acccttggct ggcggttcgt tgctgctttc catcttattc ctcccctata
      661 aactcgatga tgtcaatata ctttgtgtgg caaatctatt agtgtcaagc acaatcttcg
      721 gccatgttgc tgtcggtcaa catgtgggcg accgattggt gtcggctcgg ttcgagttga
      781 ttcaggaggg tggagcgtgc gaccatattc actcccaaaa aagggcgtgg tcgacggctg
      841 gcatgggcgt tggccgcgtc tcgattctat tttaaggtca gataatgtca ctgattgatt
      901 atttgttagg gcggcggcta gcaacttatg aagaaggaga acagcgcgtc ggcgtgatcg
      961 caggggtgcc catgctgggc ttggatgcat tggggtcggc agcgtacggg ccggaggccg
     1021 cgctgacgct tttgatacca ttaggcgcgg ccggggtgtc gtatatcggg cccatcagca
     1081 ccctgatcat tctgctcttg atggttgtct acttctccta ccggcagacg attgccgcgt
     1141 atccacacgg cggtgggtcg tacaccgtgg ccaaggacaa cctgggcacg accccgggac
     1201 tgtttgccgc ggcggcgctc ttacttgact acgtgctggt cgtggccgtc ggcatttcgg
     1261 cgggggtagg ggccctggta tcggccctgc cagtattgca gcctcatacg ctgtcgctct
     1321 gcctggcgat cctgttgttt atcacgcttg tcaacctgcg cggagttcgc gaatcggggc
     1381 tggctttcat ggctccgacc tatctgttca tcgtcacttt gctggccgtg atcgccatcg
     1441 ggatattcaa aaccgttctg gcgggcgggc atccggtgcc ggtcgtggca ccgccgcctc
     1501 tgcccagtgc cacgacggcg gcgagtcttt ggatcttgat gcgggcattt gcgagcggct
     1561 gcacggccat gacgggtgtt gaggctgtca gtaatggagt gactgccttt cgcgagccgg
     1621 ccgttcccca cgcccagcgc acgctgacgg tcatcattgg cctgttggcg gtgatgctgg
     1681 ctggtatcgc ctttttgtgc cgggcttatg ggatcggcgc gaccgagccg ggccagccag
     1741 ggtacgagag tactctctcg caactgacag cggccgtggt tggccgcggg ctgttttact
     1801 acattacaat cggttcggtg ctcagcgttc tggcactctc ggccaacact ggctttgccg
     1861 atttcccgcg gctttgccgg gtcattgccg ccgacggctt tcttcccagt ggattcgccc
     1921 accgagggcg tcgccttgtc tactcacagg gcatcattgt cctcgctgtg ctgtcggcgg
     1981 ggcttttgat cgtattcggc ggaattaccg atcatctcat ccccttgttc gcgatcgggg
     2041 cctttctggc gttcacgctt tcgcaggcag ggatggtcgt gcactggctc cgactgggtg
     2101 gggcgcatgc caagaagtcg atggtgatca acgcggccgg ggcactttct accggtgtga
     2161 cgctgggcgt cgtcgtcgta tccaaattcc tcgaaggcgc ctgggtgatg atgctgttgg
     2221 tccctgggct cttgggtctt ttctacactg tgcggactca ctaccagagg gtgggacgcg
     2281 aggttgccag cgacacacca ctcgatgccg aaggcttgac accgccggtc gtgctcctgc
     2341 cgatccgggg atggagtgca atcacgcgca aggcgctgcg gtttgccctc aaaatctcag
     2401 atgagatcta cgtgcttcat atcgcagggg acgagcagac gatggtgcgc ctcgaagagg
     2461 gctgggtgcg gctcgtcagg gagcctgcga ccaaggctgg attgcagccg cccaagttga
     2521 tcatcatcta ctccccgttt cggcagatgt acaagcctct gatcgaggtt gtttccgatc
     2581 taaagcgcgc ccaccccgat cgcgatatcg ccgtgatcat ccccgagctg atcgggactc
     2641 gctggtacca ctatgtactc cacaaccaga cggccgccgt catggtggcg taccttcgtc
     2701 tgagcggttt ccgtaaggtc gcggtgatca atgtcccctg gtacctttca gactagtgcg
     2761 gcaatggcga cggcggattg atcgccgaga ttggggacga cggggtccgc ccatgggctc
     2821 tttcatccac agccagcggc gacgtgtcga tcgacagctc ggctgcgctc atgaacggcc
     2881 ggccttcgac ctcgctcaga ccgcggagct ggacataacg cccgcgcggg ccgggaaggg
     2941 cgacatactt gaaggagggg tcgttgctcc agcgcccctt agcaacaggc ataccccagg
     3001 tcttgccatc gtcggagacc cggatctcga actccttgac gcgaccgtca ggcgaatcct
     3061 ggcgcggtac atagagaagt ccctcgacct gtcgcgggcc gctcagttca acaaccagct
     3121 cgtgcgggta tccgggcgtc gcaccgacga actcggtgcg ccagatcgtg gtaaggtcgc
     3181 cgtcgcctgc ccggcttggc tcgtagccag gcgagtagct gtcgctcgaa tacttctcgt
     3241 tacccgcgtc caaagtggcc gatcttgaag cgtcgcgaac ccactccagt atcgctcggt
     3301 aagccggatg ctcggtattt tcccatctgg gtccgcccac gtgcgaaagc ccggtgtcgc
     3361 tcgagggctc tgcctctccc tggccttggg gacttctcgg cttgcgcagg atcaagctgg
     3421 cttcgggatc tcccagattc acaaccttga gcgccgaatt gtagttgatc atgagctgtt
     3481 caccactaaa gccgcctgag gcgtcggcta cggcgatgcg gaggatcgtg tgattgccat
     3541 ggcagttcgc acaggcatgc tggtcttcac cggcttgata gaagaccggg ttgaccttgc
     3601 gccgaaaggt cgaaaagttc aagagacgag gccggcttag gggaaggtcg ggttcgatac
     3661 cggcgggatc gacgccctgg gcggtgagga tgc
//
LOCUS       DATAQL010000310         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1404269, whole genome shotgun sequence.
ACCESSION   DATAQL010000310 DATAQL010000000
VERSION     DATAQL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404269"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VHS97_05310"
     CDS             <1..1381
                     /locus_tag="VHS97_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3447647.1"
                     /translation="HVPATDWRAFLRGAFAANLPYNQLVSEILSADGTDPKTRGAAKF
                     YLDRLADPNLLTRDVSRLFLGRDLQCAQCHDHPLIDDYKQSHYYGIFAFLNRTTLFGA
                     DPAGLNKNRAVLAEKADGDVTFSSVFKKKVSHQTGPRILDGPAAPEPRVPKGAEYLIA
                     PDKANKVRPIPRYSRRGELAKRLVSAAVPQFSRNIVNRLWAIMMGRGLVHPLDLDHAE
                     NPPADPELLDLMAREFVAIGYDIKTFLREVALTRAYQRSSEPPPGASPGDEIAGDSKF
                     AVAALKPLTPEQLAWSVMQGLGLVAMTRQKAEEKLDGHDPKLRAIFQTDARRRALRIT
                     MIEESVHDQLQSDVMPFVRQFAAAAGQPQDAIEPTVHQALFLSNGRQIQTWLAPSNGS
                     LVGRLAPLADAAAVAEELYLSLYSRRPTDEERADVAQYLSERGQERVPALQELAWAML
                     TSTEFRFNH"
     gene            1455..2741
                     /locus_tag="VHS97_05315"
     CDS             1455..2741
                     /locus_tag="VHS97_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447648.1"
                     /translation="MRCSYACGSAEHIISRRSFLGTASAGLGLASGFAGLVRPAVSAE
                     LARRERQVLLIWLHGGASQLETWDPKPGTATGGPFRSIETSLPGLRISELMPLTARQM
                     HRMAVVRGVNTKEDDHGKGYYLMHTGRREAPGQEYPHLGSLAARYITSDQNPLPGYVH
                     IRPGGGGLSGREAAFLGPKYGSLVLGNGSAPANTIRPDSIDATAAAKREGLRHHLNDR
                     FAQRRRTAETDAYTTTYEQALQLMERREVFDVTKEPARDLDRYGTHDFGRHCLLARRL
                     LESGVTFVKVTHSNYDTHNENFDFHLEQVGEFDQSFSALLDDLAERGRLDHTLVVVMS
                     EFGRTPRINHLYGRDHWSAAWSVALAGAGIKPGVVLGKTNANGTAVVDGQVHAGHLFH
                     TYLRAVGLDPSETFEANGRAIQVADPAASAIKELLA"
     gene            2738..3730
                     /locus_tag="VHS97_05320"
     CDS             2738..3730
                     /locus_tag="VHS97_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3447649.1"
                     /translation="MTAEPEKTHVAKEYTHKSPLIACRFDARGRYVFASAEDSTVRRW
                     DLATGTAAALAGHDSWVFAIASDPTGDSIVTGGGDGQLIWWPATTEKPEPMRRVAAHR
                     GWVRALAISPDGSIVASSGNDRMIRLWSFADSKPLLELPGHEKPVYRLLFSPSGRTLI
                     SADLSGRVIEWDHRPGKEARRLDASKLYKYNAAGQGVDYGGVRDLALSCDGKYLACGG
                     LIEATNPLGAVSNPAVLVFDWASGTESRLLRPRENVLGLVWGLRYHPSGFLIAASGGN
                     AGGRLWFWKPEQTNEFFKLNLPNTARDMDLHPDGLRIATAHHDGKVRVSLMQAK"
BASE COUNT          716 a         1209 c         1175 g          695 t
ORIGIN      
        1 gcacgtgccg gcaacggatt ggcgcgcatt cttgcgaggc gcgttcgccg ccaaccttcc
       61 ttataaccag ctcgtcagcg agattctttc cgccgacggg accgacccca aaacccgggg
      121 agcggccaag ttttatctcg accgtctggc ggacccgaac ctgttgacac gcgacgtcag
      181 ccggttgttt ctcggtcgcg acctccagtg tgcccagtgc cacgatcacc cgctgatcga
      241 tgactacaag cagtcccatt actacggaat cttcgctttc ttgaatcgca cgaccctctt
      301 tggagccgac ccggctggac tcaacaagaa tcgcgccgtg ctggccgaga aagccgatgg
      361 tgatgtgaca ttctcgtcgg tcttcaagaa gaaggtttcg catcagaccg gcccccgcat
      421 cctggacggg ccggccgcgc cggagccgcg cgttcccaag ggggctgagt atctgatcgc
      481 tcctgacaag gcgaacaaag ttcgcccgat cccgcgctac agccggcgcg gcgagctggc
      541 caaacggctg gtgtcggccg cggtccctca gttcagccgc aacatcgtca accgactgtg
      601 ggcgatcatg atggggcgcg gcctggtaca cccgctcgac ctcgatcatg ccgaaaatcc
      661 gcctgccgat cccgagcttc tcgatttgat ggcacgggag tttgtggcga tcggctacga
      721 catcaagaca ttcctccgtg aggtggcgct gacccgggcg taccaacgat cgagcgagcc
      781 gccgccgggc gcgagcccgg gcgacgaaat tgcgggcgac tccaagttcg cggtcgccgc
      841 cttgaaacct ttgactcctg agcaactggc ctggagcgtg atgcagggtc tgggtctggt
      901 tgccatgacg cggcaaaagg ctgaggaaaa gctcgacggc catgacccca aattgcgcgc
      961 aatatttcag accgatgcgc ggcggcgggc tctgcgcatc acgatgattg aagagtcggt
     1021 gcacgaccag ttgcaaagcg acgtcatgcc gttcgtcaga cagttcgcag cggcggctgg
     1081 ccagccccaa gacgcgatcg agccgacggt acatcaggct ctgtttctta gtaacggccg
     1141 ccagatccag acttggctcg ctccgtccaa cggcagctta gtcggccggc tcgcgccctt
     1201 ggccgacgcc gcggcggttg ccgaggaact ttacttgagc ctttattccc gccgaccgac
     1261 agacgaagag cgtgccgatg tagcccagta tttgagcgag cgtggccaag agcgcgttcc
     1321 cgccttgcag gagctggcct gggccatgct aacgtcaacc gagttcaggt ttaaccactg
     1381 accacgccca gtaaaccggg cgcgcggcga gcgagatttt cgcaacgcgc gtttggtcac
     1441 gaggagaccc actgatgcgc tgctcttatg cttgcggaag cgccgaacac atcatttcac
     1501 ggcggtcgtt tttagggacg gcctccgcag ggcttggtct ggcgtcgggt ttcgcaggtt
     1561 tggtccgccc ggcggtctcg gccgaactgg cgcgacggga acggcaagtg cttttgatct
     1621 ggctgcatgg aggtgccagt caactcgaga cctgggaccc caagcccggc accgcaactg
     1681 gcggcccgtt ccgatcgatc gaaacctctt tgccagggct gcgaatctca gagctcatgc
     1741 cgctgaccgc gcgacaaatg catcgaatgg ccgtggtgcg cggggtcaac accaaggaag
     1801 atgatcatgg caagggttac tatttgatgc acaccggacg gcgggaagcg ccgggccaag
     1861 agtacccgca tttgggttcg ctcgccgcca gatacattac ctcagaccaa aacccattgc
     1921 cgggctacgt ccacatccgg cccggcggcg gcggactgag cgggcgcgaa gccgcgttcc
     1981 tggggcccaa gtacgggtcg ctcgtcctcg gtaatggcag cgctccggcc aataccatcc
     2041 ggcccgactc aatcgacgcg acggccgccg ccaaacggga aggtctgcgc catcacttga
     2101 acgaccgctt cgcccagcgg cgacggacgg cggaaacgga cgcctatacg acaacctacg
     2161 aacaggctct ccagctcatg gaacgccgcg aggtatttga cgtcaccaaa gagcccgcgc
     2221 gcgatctcga ccgctacggc acccacgact tcggccgcca ttgtttgctg gcccgccggc
     2281 ttctcgagtc cggcgtcacg ttcgtcaagg tcactcactc caactacgac acccataacg
     2341 aaaacttcga ctttcacctg gaacaggttg gcgaattcga ccaatctttt tcggctttgc
     2401 tcgacgatct ggcagagcgc ggacggcttg atcacacgct ggtagtcgtg atgtcggagt
     2461 ttggccgcac gccgcggatc aaccacttgt acggccgcga ccactggagc gccgcctggt
     2521 cggtcgccct ggcgggggca gggatcaaac cgggcgtcgt actgggcaag accaacgcca
     2581 atgggacggc cgtggtcgac ggccaggtcc acgccggcca tctattccac acgtacctgc
     2641 gggccgtggg gctcgaccct tccgagactt ttgaggccaa tggacgcgcg atccaggtcg
     2701 ccgaccccgc ggcctcagcc atcaaggaac tccttgcatg acagccgagc cggagaagac
     2761 ccacgtcgcc aaagagtaca cacacaagag cccgttgatc gcgtgccggt ttgacgcgcg
     2821 cggccgatat gtctttgcca gcgccgaaga cagcacggtc cgtcgctggg accttgccac
     2881 cggtacggca gccgcgctgg ccgggcacga cagctgggtc tttgccatcg cgtctgaccc
     2941 gaccggagat tcgatcgtaa ctggcggcgg cgatggtcag ctcatctggt ggcccgcgac
     3001 gaccgaaaag cctgagccga tgcgccgggt ggctgcgcac cgcgggtggg tcagggcgct
     3061 ggcaatcagc ccagacggtt cgatcgtggc ctctagtggt aacgaccgga tgatccgcct
     3121 ctggtcgttc gccgacagca agccgctcct cgagctgccc gggcatgaaa aaccggtcta
     3181 tcggcttctt ttcagcccca gtggtcgtac attgatctct gccgatctca gtggtcgcgt
     3241 gatcgaatgg gatcatcgtc ccggcaagga ggcgcggcgg ctcgacgcgt ccaaactcta
     3301 caagtacaac gcggcggggc agggggtcga ttacggcggt gttcgcgatc tcgccctatc
     3361 ctgcgacggc aagtacctcg cctgcggggg cttgatcgag gcgacgaacc ctttgggggc
     3421 cgtcagtaat ccagcggtcc ttgtcttcga ctgggcgtca ggcacagagt cacgactgct
     3481 ccggccgcgc gagaatgttc tgggcttggt ctggggactt cggtatcacc cgtccggctt
     3541 cttgatcgct gcatcgggag gcaatgccgg cggccggctc tggttctgga aacctgagca
     3601 aaccaacgag ttcttcaagc taaacctgcc taacaccgcg cgtgacatgg acctgcatcc
     3661 cgacggcctg cggatcgcga cggcccatca tgatggcaag gttcgggtaa gtttgatgca
     3721 ggcgaagtga gtttgatcgg acaggtgagt gcctggccac cagccatctc cttgggatcg
     3781 ttgacgttcg caaaa
//
LOCUS       DATAQL010000311         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_30608, whole genome shotgun sequence.
ACCESSION   DATAQL010000311 DATAQL010000000
VERSION     DATAQL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30608"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..2057)
                     /locus_tag="VHS97_05325"
     CDS             complement(156..2057)
                     /locus_tag="VHS97_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447650.1"
                     /translation="MAIRRRGRFVLRQIETLFNVGTIGDLTGGQFFERSVLTLTREVL
                     RSLLSIRFRWLAVVVLSAGIVCGGAMISLSGFQDSAPTKDQPVAERTTTRAAQAPIGT
                     TSERVTPDDVPLRLTLEEAVKAAGAIEDPAARRSALVRIANAQEYLSDLPAARATARL
                     AHQSAEQIASDMQRHFGLWRVAKLQAKLGDVEPARQTFAQLIKVAATKAPWDRMSLLG
                     SIAVAQNEGGLREDALQTLNRAIDDARKIVAETSKGDIYFNLVFAQCQIGDFDGVLRI
                     VESLQGKLANDRQTYLQYLARGCDKAGPAEARRILAKALELSKAIPYVYPRGLTQKMI
                     AQAMARNGDIPGALAASKLIGQIDDAPAPKGIFSIFESRQAMAKRQGEQMNAEMTRGE
                     IPDVLIAVATAQAKAGDRAAAKKTFGEAMGIIDGERDGVGKTQRLRGFVEALTAAGEL
                     IAAKVAIEAIERDEHNKALALVALGKAQARAGDKAGAIASLIAAFESAKEIKARANLI
                     NDNVAWNKDEAFRVIARAKVEVGDIAEALVTVSAHDNQSIRAEIQAEVAGFQARQGDV
                     AAAMKTAESIANASSKAEALMRIAHFQSKMGRREAAHDWAAQRGSPQERALALLGVVE
                     GVCAQRRGE"
     gene            2654..3202
                     /locus_tag="VHS97_05330"
     CDS             2654..3202
                     /locus_tag="VHS97_05330"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX3447651.1"
                     /translation="MLPLAVQPRRIIFHESSIPSRLLVSRQHICLHEPHQPKPLSDLA
                     QTRPPGRVLSKEIFMPFARIDLAQGKPPEYRATVGDVVYEGIVGILKAPDGDRFMVIG
                     EHAPENFVFDPNFLDIKRSPDLIYIQVTSTVGNTKAQKLAFFRHMADELNRRLGVRRE
                     DVFISLVFVDRDDWSFGNGEPW"
     gene            3199..3321
                     /locus_tag="VHS97_05335"
     CDS             3199..3321
                     /locus_tag="VHS97_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447652.1"
                     /translation="MKRIIQVDTVLFQEEVTGEGHRLFLGVAISQVKNGKIAVV"
BASE COUNT          647 a         1102 c         1022 g          699 t
ORIGIN      
        1 ctgggacgcc atcatcgcgc cccggatgag cagcgccacc acgcccacgg cgatcatgtg
       61 cgtccggacg cggaaacgtg gcaggcacat gcgcaggctc ccccagtgac aaacggcttg
      121 ccccggtcaa tctcgagggc gagcctttcg cgtcattatt ctcctcttct ttgagcgcaa
      181 acaccttcaa ccacgcccaa gagtgccagc gcacgttcct gcggcgagcc gcgctgcgcg
      241 gcccagtcgt gggcggcctc gcgccggccc atcttcgact ggaaatgggc gatccgcatc
      301 agagcctcgg cctttgaact ggcgttcgcg atgctctcgg ccgtcttcat tgcagcggcg
      361 acgtcgcctt gacgcgcctg aaagcccgca acctcggcct ggatctcggc ccggatactc
      421 tggttgtcgt gggcggagac ggtcaccaac gcttccgcaa tgtcgcccac ctcgaccttg
      481 gctctggcga tcacgcggaa ggcttcgtcc ttgttccagg caacgttgtc gttgatcagg
      541 ttggcgcgcg ccttgatctc cttggccgat tcgaatgccg cgatcaagct tgcaatggcc
      601 cctgctttgt ctcccgccct ggcctgggcc ttacccagtg ccaccagggc cagggccttg
      661 ttgtgctcgt cgcgttcaat ggcctcgatc gccaccttgg ccgcaatgag ttcgccagcg
      721 gccgtgagag cctcgacgaa cccgcgcaac cgctgagtct tgcccacccc atctcgctcg
      781 ccgtcaatta tccccatcgc ctcgccaaac gtcttcttgg ctgcggcccg gtcacccgcc
      841 ttggcctgcg cggtggcgac cgcgatgagg acgtcgggga tctcgccgcg cgtcatttcg
      901 gcattcatct gctcgccctg ccgtttcgcc atcgcctggc gactttcgaa gatcgagaag
      961 attcccttcg gcgccggagc gtcatcgatc tggccgatca atttcgaagc cgcaagcgcc
     1021 ccggggatgt cgccattccg agccatcgcc tgggcgatca tcttctgcgt caacccgcgc
     1081 ggataaacgt acgggatcgc tttggagagc tccagggcct tcgccaggat tctgcgggcc
     1141 tcggcggggc cggccttgtc gcaaccccgg gcgagatact ggaggtacgt ttgccggtcg
     1201 ttcgcgagct taccttgaag actctcgacg atcctgagca cgccgtcgaa gtcgccgatc
     1261 tgacactggg cgaagacgag attgaagtag atgtcgccct tcgaggtctc ggcgacgatc
     1321 tttctggcgt cgtcgatggc ccgattcaac gtctggagcg catcctctcg gagcccacct
     1381 tcgttctggg cgaccgcgat actccccagg agggacatcc ggtcccaggg ggctttggtg
     1441 gcggcgacct tgatgagctg agcgaaggtc tgccgggccg gctcgacgtc gcccaacttc
     1501 gcctggagct tcgccacgcg ccagaggccg aagtggcgtt gcatgtccga ggcgatctgt
     1561 tcggcgctct gatgggccag ccgggcggtg gcgcgggctg ccggcaggtc gctcaagtac
     1621 tcctgggcat tggcgatccg gaccagtgcg gatcgtcggg ccgcgggatc ctcgatggcc
     1681 cctgccgcct tcacggcctc ctcgagcgtg agccggagcg gcacatcgtc gggagtgacc
     1741 ctttccgacg tcgttccgat cggggcctgc gccgccctgg tcgtggtccg ctccgcgacc
     1801 ggctggtctt tcgtcggagc cgagtcctgg aacccgctca gagagatcat cgctccgccg
     1861 caaacgatcc ctgccgagag aacgacaacc gcgagccatc ggaaccggat cgacagcaag
     1921 gaccggagga cttcccgcgt gagtgtcagg acggaccgct cgaagaattg accgccggtc
     1981 aggtccccga tcgtccccac attgaagagc gtctcgatct gtcgcagtac gaagcgacct
     2041 cgtcgtctga tcgccataat atccgctcct tagccagccg gctcacggtc ccgcgccgat
     2101 ccggtatcgg ccctttcaac ataacgtata atgtcgcggg aatcgtccgc ggttctgagg
     2161 cagaccaagt agcacccgaa aggaccgggc atgtccagca atataaccga ttggtacgat
     2221 ctgagtgacg aggaaaaatc acaggttgca tctcagattt attcgccgat cggtacctgc
     2281 caaatgtcat cacggcaaga ccagcacgag aaaatcgatg tctccgtcga tcacagagcg
     2341 gccaggatag atgccaggct cgccgacata attcggtggc tgtggggaaa cggcatggaa
     2401 accaccggct gttgcgagaa tagcctgggc aagaagatag acgggtctga gtcggcctgg
     2461 atctcttttc tttcattcga gtatggagca gaccgtttca cgaaatttct aaacgcatcc
     2521 aacatccatt cggtcgtaac gaagagaacc tgcgctcccc ggttccgggt ttgcgtcgct
     2581 actccaaacg tcatgcttcg gctggctgtt gtaaactttc ggcccgacga catcgagcgg
     2641 attcggaaag cgattgctgc cgctcgccgt tcagcctcgc cggatcattt tccatgaatc
     2701 cagcattccg tcacggcttc ttgtttcccg tcagcacatc tgcctccacg aaccgcacca
     2761 gcccaagccg ctgagcgatt tggcccagac acgtccgccc ggacgggttc tatccaagga
     2821 gatttttatg ccgtttgcac gtatcgatct ggcccaagga aaacctcccg agtatcgcgc
     2881 cactgtgggt gacgtcgtct acgaggggat tgtcgggatc ctgaaggccc cagatggcga
     2941 ccgcttcatg gtcatcggcg agcatgcacc ggagaatttc gttttcgatc cgaactttct
     3001 cgacatcaag cgatcgccgg acctcatcta catccaggtg accagcaccg tcggaaatac
     3061 caaagcgcag aagcttgcct tcttccggca catggccgac gaactgaacc gccggctcgg
     3121 cgtgcgccgt gaggacgtct tcatcagcct ggtcttcgtc gaccgcgatg attggtcatt
     3181 cggcaatggc gaaccgtggt gaagcggatc atccaggtcg acaccgttct ctttcaggaa
     3241 gaagtaacgg gggagggcca ccgcctcttc ctgggcgtcg cgatctcgca ggtcaagaac
     3301 ggaaagatcg ccgtggtctg atttgtgaaa tagggcgccc agcttggttc acgacgggcc
     3361 atccggttaa cgagaaaagg ggaaataacg aatttacgaa ccgtcttttc gcggagaaaa
     3421 ggggaaataa cgaatttacg aaccgtcttt tcgggcgaga aaaggggaca
//
LOCUS       DATAQL010000312         6197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3388473, whole genome shotgun sequence.
ACCESSION   DATAQL010000312 DATAQL010000000
VERSION     DATAQL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388473"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VHS97_05340"
     CDS             complement(<1..809)
                     /locus_tag="VHS97_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447653.1"
                     /translation="MFHPFLLASYPILALFAQNVREVRSVDLVRLLVSVLLATLAVWF
                     VLALATSNVRKGGLLASAALFLFFTTDLTKGILSESLSWLSMIWVRKPIEVDPKVVII
                     MEVLLLFWGARLLRTRLNDPRRFTAFLNVFAIILLAMPMAEIISIKTPVAGRPPHEPT
                     PFKLAAPPKTARPPDIYYIILDGYARSDVMKSLFNFDNSAFLERLQKHGFYVAHQSTA
                     NYCQTPLSLSASLNAVYLDDLVRRLGADQTELTDLIGKSNVLATLRPLGYK"
     gene            complement(1333..2205)
                     /locus_tag="VHS97_05345"
     CDS             complement(1333..2205)
                     /locus_tag="VHS97_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3447654.1"
                     /translation="MRIVVTGASGHLGRYLIAGLKDGPHEIVAWSGTFQGDVEGNAVR
                     PVDLTDHRAVARALEDADPDVLIHAAAVSSAEAARRDPGRSLAVNVLATEFLSQWAAA
                     RDRRLVYTSTDLVFDGSSSWYAEEDEANPVLVYGQTKYAAERFALAMPRGLVARLSLL
                     YGPSRSGKEGYFDRATTALRAGIPQAFFTDELRTPLHYAIASRILIRLAESETQGLIH
                     VGGPERLSRYDLMRRVALALAIDPLLIQPNVRADVPLVEKRPADVSLNSSRLISLFPD
                     LDCPQIEEALSEAG"
     gene            2352..3245
                     /locus_tag="VHS97_05350"
     CDS             2352..3245
                     /locus_tag="VHS97_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3447655.1"
                     /translation="MKIREVPLDDLVLDPNLNLRDRLDDFTVERYADSWERLPPITVY
                     KVDDRLLVADGFHRHAAAVMLGKRTIRAEIIEGTFTDALDFVASVNLFHGLPLTRAER
                     RRAVEVKLKLHHDWSDRRMAEELAVSRELVAKIRRQLIESRQIPNLPGRVGADGKLYT
                     SAGLPKDAHERLPKEQPAAPEDEATERGRRGMGTAAAPLVEPKGEAPTGRGPTRAADA
                     GPAFEGGPDPRAIELSTDVGAATIDEMLELMSKQIMQVIAWTQAEGFLDAYRNASPNA
                     RGLFHTAAIKLAARADQLRRS"
     gene            complement(3344..4513)
                     /gene="hemW"
                     /locus_tag="VHS97_05355"
     CDS             complement(3344..4513)
                     /gene="hemW"
                     /locus_tag="VHS97_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX3447656.1"
                     /translation="MIAIPVQQAADPRWRWPRAAYVHIPFCAHKCGYCDFASLAGVDH
                     LADRYLTALEREIETALTEPQEVDTIFVGGGTPTRLDASSLSRLTSIIRRWFVLSPGG
                     EWTVEANPGTLDAAKADVLAEAGVERVSLGAQSFQPELLRVLERDHGRRHVEQALELV
                     RPRFPRWSLDLIFGVPGSNIDTWRADLEIALSYGPSHLSCYGLVFEKGTALWNQRQSG
                     RVEPVDEETERRMYEWTIERLAAAGLDMYEISNFAVPGHESRHNLAYWANDAYFGFGV
                     GAARYLHGVRSVNTRDINAYLRRIESGMPAIGPSEELSAEDRARETVILMLRRTQAGI
                     ERTDFERRTGFELDALAGATITRYQTRGLLEDDGQRVRLSHEGLFVADQILCDFL"
     gene            complement(4510..4785)
                     /locus_tag="VHS97_05360"
     CDS             complement(4510..4785)
                     /locus_tag="VHS97_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX3447657.1"
                     /translation="MNSSETQRDITGSRVELLERIERVLEEQVRPDLRGDGGDIILVG
                     IDPDNIVQVRLTGACQGCSSSVYTLSMRVEATLKAHIPEIRFLEAVP"
     gene            complement(4798..5145)
                     /locus_tag="VHS97_05365"
     CDS             complement(4798..5145)
                     /locus_tag="VHS97_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX3447658.1"
                     /translation="MAKRLYVGNLKYTATSEHLHELFEQFGTVTSAQVLSDRETGRSR
                     GFGFVEMANDDEALAAIESLDGHDHDGRRLTVNEARPRTSGGGSAGGGGGGGGYRAGG
                     GGYRGGGYAGGDY"
     gene            5544..>6197
                     /locus_tag="VHS97_05370"
     CDS             5544..>6197
                     /locus_tag="VHS97_05370"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HEX3447659.1"
                     /translation="MKNHLHQSLAFVVLFASGLLPALQPACADSWRVTVGGGDTDMGE
                     TPVVVALKIPLGVGIYLLESGVGGDPINAQVFEDGNGVYLAAVLPPLAAGSSHTYTLS
                     GPVAGSGSNIPGLSFEAQGRNLKVSLDQRLLTEYRVDAGNKPFFFPLVGPTGESYTRE
                     YPLSVRPDEKHDHPHQRSCWFTYGNVNGIDFWSEGKQFGTIKENERIGTIKENERTLV
                     "
BASE COUNT         1289 a         1915 c         1821 g         1172 t
ORIGIN      
        1 aacttgtagc caagcgggcg gagggtcgcc aggacgttgc tcttgccaat caagtcagtg
       61 agttctgttt ggtcggcacc aagccgcctg accagatcgt ccaagtacac agcgttcaag
      121 gaagccgaca gcgagagcgg cgtttggcag taatttgcgg tgctttggtg cgcaacataa
      181 aagccgtgct tttgcaatcg ttcgaggaat gcgctgttgt cgaaattgaa aagggacttc
      241 atcacgtcgg accgcgcgta gccgtccagg atgatgtagt aaatgtccgg cggccgagcc
      301 gtcttgggtg gcgccgccag tttgaatggg gtgggttcat gcggcggccg ccccgcgacg
      361 ggcgtcttta tcgagatgat ctcggccatt ggcatggcga gcaagataat ggcgaagacg
      421 ttcagaaagg cggtgaaccg acgtgggtcg ttgagccttg tccttagaag tcgcgcgccc
      481 cagaagagca ggaggacttc catgatgatc acgaccttgg gatcaacctc gatcggcttc
      541 cgaacccaaa tcatgctgag ccaggataat gattcgctca gaatgccttt tgtgaggtcg
      601 gtcgtgaaga acaggaacag ggccgccgag gcaagcaaac cccctttgcg cacattgctt
      661 gttgcgagcg ccaaaacgaa ccagacggcc agtgtcgcca gcaagaccga cactaacagt
      721 ctgacgagat ccaccgaacg gacctcacgg acgttttggg cgaaaagggc caatatgggg
      781 tacgacgcga gcaggaacgg atggaacacc cactttccga acgccgcccc aggctcggtc
      841 gacagccggg cggcagcatc taccgcgaac gttggcgacg gcgattcgca ctcaagtgtg
      901 ttgctggctt gcataggcag ggcacaaaat aaacgcgggc cccacaacta gcccggtttc
      961 cgtcttggga atgcgcgcga gggctatctc gatcggactc aatcggatca tcgagcaagt
     1021 ccgcttacta atcattgaac accctagccg agcaaaggtg tttctccgcg aacctgttcc
     1081 atcagttact tcgtcgccta aggtaaccag cgtcttttct gacagggtcg tccttttgaa
     1141 acggcacggg caggcggttt ttcacgctac cactcaattc tagatggccg acagctggac
     1201 ggacgtgagt atagataccg ccacaacaat gatggcaaag gtccgaaagc cactacctga
     1261 caagacattg cggacgaact gtcgggctgc gagccgacgg gttgggtgac gcacggggat
     1321 gctatttggt cattaaccag cctccgacag ggcctcttcg atttgtgggc agtcgaggtc
     1381 gggaaaaagg gaaatcaggc gcgaggagtt aagggaaacg tcagccggac gcttttcgac
     1441 gagcggcaca tcggcacgca cgttgggctg aatcaggagt ggatcgatag ccagtgccaa
     1501 cgcaaccctg cgcatcagat cgtatcgact gagccgttcg ggtccgccga catgaatcaa
     1561 tccctgcgtc tccgactcgg ccagccggat cagaatacga gatgcgatgg cgtagtggag
     1621 cggggtccgc agctcgtcgg tgaagaacgc ctgggggatc cccgcccgca gcgcggtagt
     1681 cgcgcggtcg aagtaacctt ccttgcccga tcgcgacggt ccatacagca gagaaagcct
     1741 ggcgaccaac cctctcggca tcgccagcgc aaagcgctcg gccgcgtact tggtttgacc
     1801 atacaccagg actggattcg cctcgtcttc ctcagcgtac cacgagctcg aaccgtcgaa
     1861 cacgaggtca gtggacgtgt agacgagtct ccgatcacgc gcggccgccc attgcgatag
     1921 gaactcggtt gctaggacgt tgacggccag acttctacca gggtcgcggc gagctgcctc
     1981 ggcggagctg actgctgcgg cgtggatcaa gacgtcaggg tccgcatcct cgagagcccg
     2041 cgcgacggcc cggtggtcgg tcaagtcgac cgggcggacc gcgttccctt cgacgtcgcc
     2101 ctggaacgtg ccgctccagg caacgatctc atggggtcca tccttcaagc cggcgatcaa
     2161 gtatcgaccg aggtggccgc tcgcgcccgt cacgacaatc cgcatgaccg ctaactccct
     2221 ttccttggtt tgcccaactc aaaaacttga cgcaagccac caatatccgc tgccgatcat
     2281 aggccgacag agtcggatcg aaaacaactc cgtatctgcg ctttcgcgct gacaacaaag
     2341 gaatcaccgc cgtgaagatt cgcgaagtac cgctggacga tctcgtcctg gacccgaacc
     2401 tcaacctgcg ggaccggctc gatgatttta ccgtcgaacg gtacgccgac tcgtgggagc
     2461 ggctgccgcc gatcacggtg tataaggtcg atgatcgctt gctggtcgcc gacggttttc
     2521 accgccatgc cgccgccgtc atgctgggca agcgcacgat ccgagcggag atcatcgagg
     2581 gaacgttcac agacgccctc gatttcgttg ccagtgtcaa tctcttccac ggcttaccgt
     2641 tgacgcgtgc cgaacggcgg agggcggttg aggtcaagct caaattacac cacgattggt
     2701 cggatcggcg catggccgaa gagctggctg tcagccgcga actcgtggcc aagatccgtc
     2761 gccaattgat cgaatcacgc cagattccca acctgcctgg ccgcgtcggc gccgatggca
     2821 agctctatac ctcggccggt cttcccaagg acgcccacga gcggcttccc aaagagcagc
     2881 ccgcagcccc tgaagatgag gcaaccgagc gcggacgccg gggcatgggg acagccgcgg
     2941 ctcctttggt tgagcccaag ggtgaagctc ccaccgggcg tggaccaacc cgcgccgccg
     3001 acgcaggtcc tgccttcgag ggggggcccg acccgagggc gatcgagcta tcaaccgacg
     3061 tcggagccgc cacgatcgac gagatgctcg agctcatgtc caagcaaatc atgcaggtca
     3121 tcgcctggac ccaggccgaa ggatttctgg acgcttatcg caatgccagc cccaacgccc
     3181 gcggactgtt ccataccgcc gcgatcaagc tcgccgcgcg tgccgaccaa ctgcgacgca
     3241 gttgagagag ccgtcaagac gagccattca cgctcgattt cgtctgccga cgatcccggc
     3301 cttctaggat cgcgctcgag cccacatcac gctcgcgtgt cgttcagagg aaatcgcata
     3361 agatctggtc ggccacgaac agcccttcgt ggctaagccg cacgcgttga ccgtcgtcct
     3421 cgagcaagcc acgcgtttga tatcgtgtga tggtcgctcc ggcgagagcg tcgagctcga
     3481 atcctgttct tcgctcgaag tcggttcgtt cgatgccagc ctgggttcta cgcagcatca
     3541 gaatcaccgt ctcgcgcgcc cggtcttccg ccgacagctc ctcactgggc ccgatagccg
     3601 gcattccgga ttcgatgcgc cgcaggtacg cgtttatgtc tcgcgtgttg accgagcgaa
     3661 ctccatggag atagcgcgcc gctcccacgc cgaagccgaa gtacgcgtcg ttggcccagt
     3721 aggccagatt gtgtctggac tcgtggcccg gaaccgcaaa gttagagatc tcgtacatgt
     3781 cgagcccagc cgccgccagc cgctcgatcg tccactcata cattctacgc tcggtttcct
     3841 cgtcgaccgg ctcaacccgg ccactttgcc gctggttcca aagtgcggtc cctttctcga
     3901 acacgagccc gtaacaagaa aggtgcgagg gaccgtagct caatgcaatc tcgaggtccg
     3961 cgcgccacgt gtcgatgttc gaccctggta ccccgaaaat caggtcgagt gaccagcggg
     4021 gaaagcgcgg acgtacgagc tcgagtgcct gttccacatg gcgccggccg tgatcgcgtt
     4081 cgagaactcg aagcaactcc ggttgaaaag actgcgcgcc caaactgact cgctcgacac
     4141 cggcttccgc caagacatcg gccttggccg cgtcgagagt tccgggattg gcctcgacgg
     4201 tccactcgcc cccggggctg aggacgaacc agcgcctgat gatcgacgtc agcctcgaca
     4261 gtgacgatgc atccagccgc gtcggcgttc ctcctcccac gaatatggtg tcgacctcct
     4321 gcggttctgt cagggctgtc tcgatctcgc gttcgagagc cgtgagataa cgatcggcca
     4381 aatggtcgac tcccgccaac gacgcgaagt cgcagtaacc gcatttgtgc gcgcagaacg
     4441 gtatatgaac gtatgcggca cgaggccagc gccacctcgg atcagcggcc tgctggacgg
     4501 ggatagcgat cacgggaccg cctcgaggaa ccgaatttca ggtatgtgcg ccttgagggt
     4561 tgcctctacc cgcatgctaa gagtatacac cgacgacgaa cacccctgac atgccccagt
     4621 cagccgaacc tggacgatgt tgtccgggtc aataccgacc aggatgatgt cgccgccgtc
     4681 acctcggaga tccgggcgga cctgctcttc gagcacgcgt tcgattcgct cgagcaattc
     4741 cacgcgcgag cccgtgatgt ctcgttgagt ctcgctgctg ttcatcatag ttgccagtca
     4801 gtagtcgcca ccggcatagc cgccaccgcg atagccgccg ccgccggcgc ggtagccacc
     4861 acccccaccg cctcctccgg cgcttcctcc gccggaagtt ctcggccggg cttcgttgac
     4921 cgtcaaccgg cggccatcat ggtcatggcc gtcaaggctc tcgatcgccg ccagggcttc
     4981 gtcgtcgttc gccatttcca cgaaaccaaa tccccggctc cggccagtct cgcggtcgct
     5041 cagaacctgc gcagacgtga cggttccgaa ctgctcgaac aattcatgga ggtgttcgga
     5101 tgttgctgta tacttgagat ttccaacata aagccgcttc gccatcgcag tcgcccgaaa
     5161 aaggctctcc accgaccttc aactgtagac cgatacgtta agatacgtta attcggaagc
     5221 caccgtagcg gcgaccggcc ccacgatgga tccacccgac accccgatcc aggcgacaac
     5281 gaagtgcagc cacgtagatc gctctccgca gtgtgcccag atcgttgcaa gaaactataa
     5341 gcactcggtt gcttcgcatc aacgacaaaa tgacaatcag tttaaatcgt acgggcttgc
     5401 gtccaacagc gcatgatcga gcgaatgcgc gattggcgtg gcgggtgtgg cccgaccaat
     5461 tccggtcgag aactggtttc acgaaaatgg tggcgataca atcgagccgc ggcgcttaca
     5521 agcgatcaga cgagggcgga tcaatgaaga atcacttaca ccaatcgctg gcatttgtgg
     5581 tcttgttcgc gtctgggctg ctaccggctc tccagccggc ctgtgcggat tcctggcgcg
     5641 tgacagttgg cggaggcgac accgacatgg gcgagacgcc cgtggtggtc gcgctcaaga
     5701 taccgctcgg agtcggtatc taccttttgg aatcaggcgt cggcggggat ccgataaatg
     5761 cccaggtctt tgaggacgga aacggcgttt acctggcggc ggtcttaccc cccctggctg
     5821 caggctcgtc ccacacgtac acactcagtg ggccggtggc cggcagcggc tcgaacatac
     5881 ccggcctctc gttcgaggct caaggacgga acctcaaagt cagcctcgac cagcggctct
     5941 tgaccgagta tcgcgtcgat gccggcaaca agccattctt cttccccttg gttggaccga
     6001 ccggcgagtc atacacgcgg gagtatccct tgtcggtccg tcccgacgaa aaacatgatc
     6061 atcctcacca acggtcgtgc tggttcacct atggtaatgt taatggcatt gatttttggt
     6121 cggaaggcaa gcagttcggg accatcaagg agaatgagcg gatcggaacc atcaaggaga
     6181 acgagcggac tttggtc
//
LOCUS       DATAQL010000313         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_489636, whole genome shotgun sequence.
ACCESSION   DATAQL010000313 DATAQL010000000
VERSION     DATAQL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489636"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VHS97_05375"
     CDS             complement(<1..282)
                     /locus_tag="VHS97_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447660.1"
                     /translation="MRFKRSNVIGIVVLFGIGSGSCAWHAFAAAFDNTGTAQAGSGQS
                     VGKSNGEPPGSPPKAPTPVTRLTGTVKLDGTGQPIAGAKLQILSGLIMGL"
     gene            complement(525..1280)
                     /locus_tag="VHS97_05380"
     CDS             complement(525..1280)
                     /locus_tag="VHS97_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3447661.1"
                     /translation="MPDQVSVVRVAAVGDLHCNKNSQGLIRPLFENSDQFADVLVLCG
                     DLTDYGLPDEAHLLVEELSVASKLPIVAVLGNHDFESGREDEVRQILVDAGIILLDGD
                     AVEVRGIGFAGARGFAGGFGRGTLGSWGERVVKAFVQEAVDEAMKLESALARLRTQSR
                     VAVLHYAPIRATVENEPAEIFAFLGCGRLEEPLNRYPVTAVVHGHAHNGSAEGRTTTG
                     VPVYNVSLPLMRRILPDRHPFLVLELPAAPAGP"
     gene            1425..>1788
                     /locus_tag="VHS97_05385"
     CDS             1425..>1788
                     /locus_tag="VHS97_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447662.1"
                     /translation="MELAPVGLNPQTRAFYVRTLAVLNEAGVPYLVGGAYALAKHAGI
                     ERHTKDLDIFVRRGDRDAILRALELAGYRTEVSFPHWLAKAYGDDGFIDVIYSSGNGV
                     VKVDEEWFSHASDGEILGV"
BASE COUNT          338 a          541 c          554 g          355 t
ORIGIN      
        1 taaacccatg ataaggccac tgaggatttg tagtttcgcg ccggcgatcg gctgcccggt
       61 cccgtcaagc ttcaccgttc cagtcaaccg ggtaacgggt gtcggtgcct tgggcggcga
      121 acccggtggt tcaccgtttg acttgccgac cgattggccg gatccagcct gagcagtgcc
      181 cgtgttatcg aacgccgcgg caaacgcatg ccaggcgcac gaaccgcttc caatgccgaa
      241 aagaaccacg attccgatga cgtttgaccg cttgaatcgc atggaactta ggcctcctca
      301 cgcacagatt gcttcgacga tattggtgcc aaagtcttgg tctcggcctc gacaacaaca
      361 gcttgcgctg caggctgtaa gatcgcaagt tagatctgtg gttcagattg ttagctaatt
      421 tccgtatctt gattccttct cctttcggcg acgccttatc cctcataggt catcgcgccc
      481 gccgtatcca tgaacggtct cgtgtccacc atgtcggcgc ggtgctacgg cccggcaggg
      541 gcggcgggca attcgaggac caggaacggg tgtctgtcgg ggagtatgcg gcgcatcaag
      601 gggagcgaca cgttgtagac cgggaccccg gtcgtggtgc gcccttccgc gctgccattg
      661 tgcgcatgac cgtggacgac ggccgtcacc ggatagcgat tcagcggctc ttccaggcgg
      721 ccgcagccga ggaaagcgaa gatctcggcc ggctcattct cgacggtcgc ccggatcggg
      781 gcataatgga gcacggcgac acggctctgg gtgcgcagcc tcgccaaggc ggattcgagc
      841 ttcattgcct cgtcaaccgc ttcctgaacg aatgctttga ccactctctc gccccaagag
      901 ccgagcgtcc cccggccgaa gccgccggcg aagcccctgg cccccgcgaa cccgatcccc
      961 cgtacctcga cagcgtcgcc gtcgagcagg atgatccccg cgtcgaccaa gatctgccgg
     1021 acctcgtctt cgcgacccga ctcgaagtcg tgattgccca gcacggcaac gatcggcagt
     1081 ttcgaggcga cggacagctc ttccaccaac aggtgtgcct catcgggcag gccgtaatcg
     1141 gtcaggtcgc cgcagagaac gagcacatcg gcaaactggt cggaattctc gaaaaggggg
     1201 cggatcaagc cctgggaatt cttgttgcaa tgcaggtcgc cgactgccgc gactcgcacg
     1261 accgatactt gatctggcat cattccctcc aattctcggg atgatctgac gccgcacaga
     1321 gcgggcggct ctcgtcaagt cggcaaagtg cgtgcccaga cggcactggc tttgcagtga
     1381 tagattgggt tgcgtctttg tccgcgggcg gagggccgtt cggtatggaa ctggcaccgg
     1441 tcgggttgaa tccgcagaca cgagcgttct acgtgcggac gctggccgtg ctcaatgagg
     1501 cgggcgtgcc gtaccttgtg ggcggcgcgt atgcgctggc aaagcacgcg gggatcgaac
     1561 gtcacacgaa agatctggac atctttgtgc gaaggggtga ccgcgatgcc atcctcagag
     1621 cactcgaatt ggctgggtat cgaacagagg tttcgttccc acattggctc gccaaggcgt
     1681 acggcgacga cgggttcatc gacgtgattt atagctcggg caacggcgtg gtcaaggtcg
     1741 atgaggaatg gttctctcat gcatccgacg gcgagatctt gggcgtcc
//
LOCUS       DATAQL010000314         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_336153, whole genome shotgun sequence.
ACCESSION   DATAQL010000314 DATAQL010000000
VERSION     DATAQL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336153"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3292)
                     /locus_tag="VHS97_05390"
     CDS             complement(<1..3292)
                     /locus_tag="VHS97_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretory pathway, component PulD"
                     /protein_id="HEX3447663.1"
                     /translation="MGRLMTVSLILILSAWGGAARAAAPDDAAAAPTSATHQQPPAPP
                     IKYLEAGARLFNSAVDSTQLDLATKYLQAANTYRDQLQPEEQATLDAYLKELAKAKAV
                     VASGSKAVAPGGTGTEPAIPSAAVPPAAGPAAPVVSNQLPPTVDTKQRGRWLLHEARE
                     QLHVGNYDMAQQKVDEADALDIKWGLFDDTPAKVTEEIKKARPKMAASTAKTAVNQPH
                     DRRNARIKLQEARAALNNRQFEQAEVIALDVKKWGLTYGLFEDNPDKVAAAARALRRR
                     DKIRNTTPREQSSQGVYDILVQESRQLMSVGNLDLAEAKARQAQRMNVVPPLTADRAE
                     SVLHEIAMARVQKAPAATAARPATEAPSLKLEREANELLAKGDQNAAAAKFSEADRLS
                     VGESLAAVDAKAAVPARPVPDPAVQKMAVAAPASAPDLAAPMDNPVAQQTAPAPAPVA
                     QQTAPAPAPVAQQEPTVDPAPATTLAVGNRGEQLLGEAKQLYKNGNYQAAKQMAVEAK
                     AGKFGVEAQADELITQIGLAEQGGALSLYEAALSALRSGDNGRARALLTEVAAAGESL
                     DDSMRTKVENLLQKLANDDKGQPEGKSSTHTSQDAEALAAQRLNAEVGTKIAEGRRLH
                     ETDPNKAIAIYEKTVQAVQAAGLSPELTRPMVRRLEVAIELATKDKVAFEVKMQDKQL
                     RAEIETKRLRILEADKAKKVRLKELMDKAMTAYAEGNYVESEALAKRAMEVDPNELAA
                     SMLVFKAKAERRFKQDQQNKNDKDEGVVQMFQGVDMAAIADPEVQMRDIKFPKTFKDL
                     TESRLRMNAKLEPRKDPKVLAIEAKLKDRISMNMDKQPLSEAVTFLQNYTGLNVVLDS
                     KALADEGLSSASPVSLVVNQVKLRTALKLMLQPLGLTYKLEDDVVLITSPLATQAQTI
                     TQTYYVGDLVMPPDHGPQNLLPHAAGFPEQGSQNDANPGTGPSQGVYGSSDPRFPQQP
                     FNGIGNATGKRPTVDMTPIIQLITSSIAPGTWRVQDGYGQDATSAYGLGGGFGGDAGG
                     IDQQRQPGAIVPFMLSISLIIKHTAEVHDQVADLLRQLRRLQDLQVSVEVRFITVQDT
                     FFEFIGVDF"
     gene            complement(3801..>4434)
                     /locus_tag="VHS97_05395"
     CDS             complement(3801..>4434)
                     /locus_tag="VHS97_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447664.1"
                     /translation="RIVVFNPNKGRDDISPGVDNKSDELRGDWSESTDQVTMPPDVMP
                     YVTATSPPGPGNDMKVRFQVIRFHPADGVTVPRNFDAAPGEVIGEPRTSEIPVSDGTG
                     KKSKVIDFNSRQIMLDVFAIKKTGGFQHLPAGFVGPPIARPALALVLRPDGSVAVHNE
                     VDDESNEVRKDIDTNYHHELDQSGKKRESSRGMGYMGGMMGRMGGGGMGR"
BASE COUNT          753 a         1357 c         1331 g          993 t
ORIGIN      
        1 cgaagtcgac gccgatgaac tcgaagaacg tatcttggac cgtgataaac ctgacttcga
       61 ccgacacctg aaggtcctgg agccggcgaa gctgccgtag caggtcggcg acttgatcgt
      121 gaacttcagc cgtgtgcttg atgatcaagc tgatgctgag catgaaggga acgattgcac
      181 cgggctgtcg ttgctggtcg atgccgccgg catccccgcc gaagccgccg cccaggccat
      241 acgcggaggt tgcgtcctgg ccatagccgt cttgcacccg ccaggtgccc ggcgcgatcg
      301 acgacgtgat caactgaatg atcggcgtca tgtcgactgt gggccgcttg cctgtggcgt
      361 taccgattcc attaaatggt tgttgcggga atctggggtc cgacgacccg tagacaccct
      421 ggctaggccc ggtgccgggg ttggcgtcat tctgcgagcc ctgctcggga aaacccgcgg
      481 cgtggggcaa caagttttga gggccatgat ccggtggcat gaccaggtcg ccgacataat
      541 acgtctgcgt gattgtctgt gcctgggttg ccagggggct ggtaatcaaa accacgtcgt
      601 cctcgagctt gtaggtcagc ccgagcggct ggagcatcag tttcaaggcc gtccggagct
      661 tgacctggtt aacgaccaag ctcaccggcg aagcggacga caatccctca tccgccagcg
      721 ccttggaatc aagcacgacg ttcaagccgg tgtaattttg aaggaaggtg acggcctcgc
      781 tcaaaggctg cttgtccatg ttcatcgaga ttcggtcttt gagcttcgcc tcgatcgcca
      841 ggaccttggg gtctttcctg ggctcgagtt tcgcgttcat tctcagccgg ctctcggtca
      901 agtctttgaa cgtcttgggg aacttgatgt cccgcatctg gacttccgga tcggcaatcg
      961 ccgccatgtc cactccctgg aacatctgga cgacgccctc gtccttgtcg ttcttgtttt
     1021 gctggtcttg cttgaagcgc cgttccgcct tggccttgaa gaccaacatc gacgcggcca
     1081 gctcgttggg atcgacctcc atggcacgct tggcgagggc ctcggactcg acgtagttac
     1141 cctcggcata agcggtcatg gctttatcca tcaattcttt caaccgcact tttttggctt
     1201 tgtcggcctc caggatacgc agccgttttg tctcgatctc ggctcgcagc tgcttgtcct
     1261 gcatcttgac ctcgaaggcg accttatctt tcgtggcgag ctcgatcgcc acttcgagcc
     1321 ggcgaaccat gggccgggtc aattcgggcg aaagtccggc ggcctgcacg gcctgcactg
     1381 tcttctcata gatcgcaatc gccttgttcg gatcggtctc gtgcaggcgt cgaccctcgg
     1441 cgatcttggt cccgacctcg gcgttgagtc tttgggccgc gagtgcctcg gcatcctggg
     1501 aagtgtgggt gctcgactta ccctcgggct ggcccttgtc gtcgttggcc agcttttgaa
     1561 gcaggttttc gaccttcgtt cgcatgctgt cgtcgagtga ctcgccggcg gcggcaacct
     1621 cggtcaagag ggcacgtgcc cgtccgttgt ctccgctacg tagcgccgag agcgcggcct
     1681 cgtacaggct cagtgccccg ccttgctcgg caagaccgat ctgcgtgatc aactcgtcgg
     1741 cctgagcttc aacgccaaac ttgccagcct ttgcctcgac cgccatctgc ttggcagcct
     1801 gataattgcc gtttttgtac aactgcttgg cctcgcccag caactgctcg ccgcgattgc
     1861 cgacagcgag ggtggtggcg ggtgcgggat cgaccgttgg ttcctgttgg gcgacggggg
     1921 ccggtgccgg tgcagtctgc tgcgccacgg gggccggtgc cggtgcagtc tgttgcgcca
     1981 cggggttgtc catcggcgcg gccagatcag gtgctgatgc gggcgcggcc accgccattt
     2041 tctggactgc tggatcgggc accggccggg cagggacagc ggccttggca tcgacagccg
     2101 cgagcgattc gccgacgctc aaccgatccg cctcggagaa tttggcggct gcggcatttt
     2161 gatcgccctt ggccaagagc tcgttggcct cgcgctccaa cttcaaacta ggcgcctctg
     2221 tcgccggtcg cgccgcggtc gccgccggcg ccttttgcac tcgggccatc gcgatctcgt
     2281 gcagcaccga ctcggcacga tccgccgtca gcgggggcac cacgttcatc cgctgggcct
     2341 gacgtgcctt ggcctcagcg aggtccaggt tgccaaccga catgagctgg cgggattctt
     2401 gaaccaggat gtcatacact ccctggctcg actgctcacg cggcgtcgtg ttgcggatct
     2461 tgtcgcgccg acgcagggcc cgtgcagcgg ccgcgacctt gtcgggattg tcctcaaaca
     2521 ggccgtaggt cagtccccac ttcttgacat cgagcgcgat tacttccgcc tgttcaaact
     2581 ggcggttgtt gagtgcggcc cgagcctcct gaagcttgat ccgcgcgttc cgtcgatcgt
     2641 gcggctgatt taccgcagtc ttcgcggtcg aagcggccat tttgggcctg gccttcttga
     2701 tttcctcggt gaccttggcg ggcgtgtcgt cgaatagccc ccacttgatg tccagcgcat
     2761 cggcctcgtc aaccttttgc tgggccatat cataattgcc cacgtggagc tgctcacggg
     2821 cttcatggag caaccacctg ccccgctgct tggtatccac agtgggaggg agttgattgg
     2881 aaacaacagg ggcggccggt ccagcagccg gcggaactgc ggccgacgga attgccggct
     2941 cggttccggt tccgcctggg gccaccgctt tagaacccga ggccacgact gccttggcct
     3001 tggctagctc tttcaagtag gcatcgagcg ttgcctgctc ttctggctgc agctgatcgc
     3061 ggtacgtatt agcggcttgt aagtatttgg tggccagatc cagttgggta ctgtccaccg
     3121 cgctgttaaa caaacgcgct ccagcttcca agtatttgat cggtggcgcg ggcggctgct
     3181 gatgggtcgc cgaggttggc gcagcggccg cgtcgtcggg ggccgcggct cgagcggctc
     3241 ctccccatgc gcttaggatc aagatgagac tgaccgtcat aagccttccc aacgcgacct
     3301 cctttcgccg ctcgcgacga cggccgccga cttccctggc gacggcccat gttttgtcat
     3361 cacaagtggg agaccaatag ccgaatcggt tgatcgcgcc actccacaag tgcggaatgg
     3421 ccgcttcacc taaatcaatt ggaaacgtgg cgaaatcatg atgtggtcgc gagatggcaa
     3481 acccggaacg gggagaagat gtgacggtgt gtactctcaa gggactctcc ggtcaagacg
     3541 agttccacga attattcgct tccgtcgtta acttgatcgg ctaaatccac cgtcaaactt
     3601 tgcccacgca tcgctttgat taatttcggc acgatcgctc ccgatcgtta catgtcgaat
     3661 cgtgcgcaac aaaaaaaaac ccccacagga atgtgggggt cgagccgctg catgcttcga
     3721 gtcgtcgact accagtcgtc gacctctggg cgcctaccat cctttggctt gtccgaagtc
     3781 ggcgagggaa agggggagac tcatctgccc ataccgccac cacccatgcg tcccatcatg
     3841 cctcccatgt aacccatccc cctgctgctc tcgcgtttct tgcccgactg atcgagctcg
     3901 tgatgatagt tggtgtcaat atcctttcgg acctcattcg attcatcgtc gacctcgttg
     3961 tgcacggcaa cagagccatc gggccgcaat accaacgcca gggccggccg cgcaatagga
     4021 ggacctacga atccagcggg taggtgctga aagccgccgg tcttcttgat cgcaaacaca
     4081 tctagcatga tctggcgact attaaagtcg atgactttgc ttttcttacc cgtgccatcg
     4141 gatacgggaa tctctgaagt ccgcggctcg ccgatgactt ctcccggtgc ggcgtcgaaa
     4201 ttacgaggca cggtcacgcc gtcggcggga tggaagcgga tgacctggaa tcgtactttc
     4261 atgtcattac cgggacctgg aggcgaggtt gccgttacgt agggcatgac atcgggaggc
     4321 atggtcacct ggtcggtcga ctcgctccaa tccccgcgca gctcgtccga cttgttatcg
     4381 acgcctgggc tgatgtcgtc acgacccttg ttggggttga agacaacgat gcga
//
LOCUS       DATAQL010000315         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5374308, whole genome shotgun sequence.
ACCESSION   DATAQL010000315 DATAQL010000000
VERSION     DATAQL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5374308"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..>1843
                     /locus_tag="VHS97_05400"
     CDS             231..>1843
                     /locus_tag="VHS97_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3447665.1"
                     /translation="MALLSLRNVSLAFGGPRLLDQVELQIEPGERVCLLGRNGEGKSS
                     LLRLIRGEIEPDEGQVIRQQGLRVSFLPQDVPRERTGTIADQVAEGLDDSDHHAAGSD
                     HRVQAVLSRMGLDPDARFEALSSGMKRRVLLAQSLVSEPDILLLDEPTNHLDIDAIRW
                     LEGFLPRAGGTLIFVTHDRVFLEKLATRIVELDRGQLYDWACDYPTFLKRRDEVLHAE
                     ARQQDLFDKRLAQEEVWIRKGVEARRTRNEGRVRALKAMREQRQKRRDRTGTARIQAQ
                     EAERSGTLVIDAKGVGFGYGGDPVIQDLTTTITRGDKIGIIGPNASGKTTLIRLLLGE
                     ITALSGTIRHGTRLEIAYFDQLKATLDEEKTVQQNVSEYDTIPINGQPRHVIGYLQDF
                     LFAPERSRSLVKYLSGGERSRLLLAKLFTKPSNLLVLDEPTNDLDVETLELLESLLVE
                     YQGTVLLVSHDRAFLNDVVTSTLSIEADGRVREYDGGYDDYMRQRSAELPPEPKSSTA
                     GGNLPASPTAEKRKKLSFKERRELESLPAR"
BASE COUNT          390 a          565 c          551 g          337 t
ORIGIN      
        1 accatctgcg gtgtatttct cgtcgccacc tagggcggat ccggacatgg acgctgaaat
       61 ctgagttcgc aaccaccgcg ccgtttgaga atccgttggc gcaccccggc ggcgccggtc
      121 gaccaaatcg cctcgctgat cggtctcagg atttgttacg gtggcggcat tgccccgcgg
      181 tactgggcct cggtcggccc acctgaagtt acacgagtcc gagcgcactt atggcattgt
      241 tgagtctgcg aaacgtcagt ctggccttcg gaggccctcg tctcttagac caagttgagc
      301 tgcagatcga gcccggagaa cgggtctgct tactgggtcg caacggtgaa ggcaaaagct
      361 cacttctccg cttgatacgc ggcgagatcg agcccgacga aggccaggtc atccgccagc
      421 aagggctacg ggtctctttc ttgccccagg atgtcccgcg tgagcgaacc ggcacgatcg
      481 ccgaccaagt tgccgaagga ctggacgaca gcgaccatca cgcggccgga tctgatcatc
      541 gcgtgcaggc tgtgctttcc cgtatgggtc tggaccccga tgcgcggttt gaagctctct
      601 cctccggaat gaagcgacgc gtcttgctcg cacagtcact cgtaagcgag cccgatatcc
      661 tcctgctcga cgaaccgacg aaccatctcg atatcgacgc gatccgctgg ctggaaggtt
      721 tcttgccgcg ggccggcggc acattgatat tcgtcacgca cgaccgggta ttcctggaaa
      781 aactagcgac gcggatcgtc gagctcgacc gcggccagct ttatgactgg gcgtgcgact
      841 atccgacgtt tctcaagcgc cgcgacgaag tcctccacgc cgaggctcgg cagcaagacc
      901 tgtttgataa gagactcgca caagaagaag tgtggatcag aaaaggtgtc gaggcgcggc
      961 gcacccgcaa cgaaggacgt gtccgagcac tgaaagccat gcgagagcag cgccagaaga
     1021 gacgcgatcg caccggcacc gcgcggattc aggcacaaga ggcggagcga tcgggcaccc
     1081 tggtcatcga cgccaaaggc gtcggcttcg gctatggcgg cgacccggtg attcaggatc
     1141 tcacaacgac gatcacgcgc ggggataaga taggaattat cgggcccaac gcttccggca
     1201 agaccaccct gattcgactt ttgctcggcg agatcaccgc gctttccggc acgattcgcc
     1261 acggcaccag gctcgaaatc gcctacttcg atcagctgaa agcgacgctc gacgaggaaa
     1321 agaccgtcca gcaaaatgtc agcgaatacg acaccatccc gatcaacggc cagccacggc
     1381 atgtcatcgg ctatctccag gattttctct tcgcgcctga gcggtcgcgc agtctggtga
     1441 agtacctttc cggcggcgaa cgcagccgac tgctgctggc gaaactattc accaaaccct
     1501 ccaatctgct tgtactggac gagccgacga acgatttgga cgtagagacg ctcgagctct
     1561 tggagtcgct cctggtcgag taccagggga ctgtgctcct ggttagccac gaccgcgcct
     1621 ttctgaatga tgtcgtcacc agtacgcttt ccatcgaggc cgacggccgg gtgcgcgagt
     1681 acgacggcgg ttacgacgat tacatgagac aacgttcggc cgagctgccg ccggagccga
     1741 agtcatcgac tgccggaggc aatctgccgg catcaccgac ggcggaaaaa cgcaagaagc
     1801 tgagtttcaa ggaacggcgc gagttagaat cgctgcccgc gcg
//
LOCUS       DATAQL010000316         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4000117, whole genome shotgun sequence.
ACCESSION   DATAQL010000316 DATAQL010000000
VERSION     DATAQL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000117"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VHS97_05405"
     CDS             <1..302
                     /locus_tag="VHS97_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HEX3447666.1"
                     /translation="MPVAIEQHVDFITDCIAHMHARRLSCVEATPDATEKWVSQVNEA
                     ASVTLLPQAKHSWYLGANIPGKPQVFMPYAGGMARYRAICAGIARSGYEGFALSP"
     gene            complement(518..1399)
                     /locus_tag="VHS97_05410"
     CDS             complement(518..1399)
                     /locus_tag="VHS97_05410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX3447667.1"
                     /translation="MQIGYFAVGIGPTVDPERVRTVATTAERLGFATIWAPEHVVLLE
                     QYASKYPYSSGEFPAPTDTPIADPFTTLAYAAACTSKIRLATGICLVPEHNPLVLAKT
                     VATVDLLSGGRFILGVGVGWLAEEFEALGVPFERRGQRTREYIEVMRKLWTERSSSHQ
                     GEFVKFTNVLSYPKPVSDRSVPVWFGGESNPALRRVAEYGDGWIGFNLLPDQAAEKIK
                     RIEELLKANGRNRSDVKLAVSPYTNPIKTDDLKRYRDAGVEEIALVRLGRPGTEREMI
                     AQLEQIAREFVEPAAKL"
BASE COUNT          422 a          688 c          623 g          437 t
ORIGIN      
        1 acatgccggt ggccatcgag cagcatgtgg acttcattac cgactgtatc gcccacatgc
       61 acgcgcgccg actctcctgc gtcgaggcga cgccagatgc gacagagaaa tgggtgagcc
      121 aggtcaatga ggccgccagc gtaaccctgc tgcctcaggc gaaacactcc tggtatctgg
      181 gcgccaacat ccccggcaag ccgcaagtgt ttatgcccta tgccggcggc atggcgcgct
      241 accgagcgat ctgcgccggc attgcgcgca gtggatacga ggggttcgcc ctgagcccct
      301 aagcccgcca tgaacgtggc ggcgccgggt cacgcatgcc gtcgagcaac tcgccaacaa
      361 aacaccgccc ggcccggtgc actgacctga cggctctgcc tcgcccgcag ctccccccgg
      421 ttcgcctctg ggtggaacac ggtgataagg cggtgtttcg gggaagtgcc gagtcctccg
      481 cgtcctccgc ttcctccatc gagccgattg gctgccgtta gagcttggcc gccggttcga
      541 cgaactcccg cgctatctgc tcgagctgcg cgatcatttc gcgctcggtc cctggcctgc
      601 ccaacctcac cagggcgatc tcctcgacac ctgcatcgcg atagcgtttc agatcgtcgg
      661 tcttgatcgg gttggtgtat ggcgacaccg cgagtttgac atcggagcgg ttgcgcccgt
      721 tggccttgag caattcttca atgcgtttga tcttctcggc ggcctgatca ggcaagaggt
      781 tgaagccaat ccagccgtcg ccgtactcgg ctacgcggcg aagcgcggga ttgctctcgc
      841 cgccaaacca caccggtacg cttctgtcac tgacaggctt ggggtagctc aacacgttcg
      901 tgaatttgac aaactcgccc tgatgcgagc tgctccgctc cgtccagagc ttgcgcatga
      961 cttcgatata ctcgcgcgtg cgctgaccac gacgctcgaa cgggacgccc agagcctcga
     1021 actcttctgc cagccatccg acgccgacgc cgaggatgaa ccggccgcca ctcaacagat
     1081 cgactgttgc tacggtcttc gccagcacga ggggattgtg ctcgggcacg agacagatac
     1141 ccgtcgccag ccgtatcttg cttgtgcagg cggccgcata ggccaatgtc gtgaaggggt
     1201 cggcgatcgg ggtatccgtg ggtgccggaa attcaccact cgagtacgga tacttggacg
     1261 catattgttc gagcaggacg acgtgctccg gcgcccatat tgtagcaaaa ccgagccgct
     1321 ctgcggtggt ggcgaccgtc cttacccgtt cgggatcaac cgttggcccg ataccgactg
     1381 caaaatatcc gatctgcatg agtcatactc cacgacgaga tgggtcagac ctggagcgat
     1441 ctcaggattg agatcagcga cggcttcgat tctttcaggt gaacgcggcg ccgtaaagtc
     1501 tacccccacc aatcccgtgg ggctcgcctc atcatttccg agccgggcaa aaatgcgttt
     1561 catcgccggt gaccgatgac gtggagagct tgccggccaa cgggcgtaaa gcggaggcgc
     1621 cgccgccggc cgtcgagacg cgccgctgcg acggatgcga cgccgatttc cggcccgccc
     1681 gcccatggtc gcgcttttgt cggctgcggg ctcatggcac ccttcgcagc acatttccaa
     1741 aatcatcccc cgaaccggtg gataactttg ttgaaaactc gccaccggac aggtcgtaat
     1801 gaggcaatat tagcgattta aaggtttttg cctaaaattt catcagatag ttaaggcact
     1861 gattagattt atatttttaa tgtcaacagt gattaacctc ttaaatatct gaaggccaaa
     1921 gcctgtttgg gacctgtcgc atctatgtca caattcctgt gagcgcgaga tcgccgggtg
     1981 acccggccga cgccgttgcc gattgggcgc cgggacgctc acgctccggc gcgaaccctc
     2041 gctgacgaaa acggttgcac atccgagtcg cgagcaaaca cggcaacagc tatgctcgcc
     2101 tgacactggc ccgttccgac gccctcatac gagaggatct cccatgctgg agctttcgga
     2161 catccaatct
//
LOCUS       DATAQL010000317         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4152936, whole genome shotgun sequence.
ACCESSION   DATAQL010000317 DATAQL010000000
VERSION     DATAQL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4152936"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VHS97_05415"
     CDS             complement(<1..1037)
                     /locus_tag="VHS97_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEX3447668.1"
                     /translation="MAEELDKYEEDVDRIIDTGKEKGYLTFGEVNDLLPGDITSPDDL
                     DDLLTTINTQGIDVLSSDGRRGTPGERDEEHEAGEESDDVELDLSPGTLEKTNDPVRM
                     YLREMGTVPLLTREGEVEIAKRIERGQMRVMKAISRSPIVIREITGLGEDLKRGVRNI
                     KEVVVFDEEELTEEILAARVKQTVSRIDELIKHQKKAADFGARAAEPHGKDAKAKEKD
                     KRKSRWLMGREQVFVNRIVRELKYTNGEKKRLLDKVNKTVDSMRTLERQIRSLEAKFE
                     ASRSEELKKEYKRQQKNCRIDLDRVEADAGISLVDLKRTQREMIQGDMDAERAKRELI
                     EANLRLVVSIA"
     gene            1499..3541
                     /locus_tag="VHS97_05420"
     CDS             1499..3541
                     /locus_tag="VHS97_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447669.1"
                     /translation="MKKLLSLPLTPILLAGVAAGLLTGCSSSFAPNPIESDQTPIGNI
                     QGRVHGGQYPISGAHIYLYAAGTTGYGTAATSLICNTGSTCGSNAYQDGSGNYYVTTD
                     SGGNFALGGEYTCTVGTQVYMVATGGDQQLSSVPAYNTAIVQMAALGQCPTAGNLAQQ
                     VPFLTINEVTTVAFAYAMSGFATTPYNVSSNAANSTASKIAIANAMANAGNIVNLQWG
                     QAPDKANGNSNSTNPQTKIYALANILSTCVNTTGNTSAGCQALFTAAKDSSGNASTDE
                     ASAIFNIAHNQGQNVAAIWKLNPKNPVFSPTLSGTTGPNDWTLPVVYTGVCSAFGTNG
                     SNQVTSGAYNIAFDVNGTAWIGDRQKGVIEMTPLGAVSHTYNPSFSMVKGIAISPDST
                     QIWVADYSGGTNKTGQVDIMSTAGAITTSMTTDIDGPSSIALDPKGYAFVANETNGSV
                     VAFSSAGGFIDYSGAVGVTTPAWISVDVTGNVWLPSTSGNTTQVVAQVPLNYAGNSGK
                     FKGFGTAATIAADWSYGVAIDSNDYAWFATNDLSILGSTPSNENLEDIVLTPSTNKKG
                     KTTYSYQISSTESGSGTNGGGLGIPYKISIDGGNNIWMANEYYSTVSGYSETLSSWLI
                     KGTAFSNGTTVGTLSATPDNSGNLWTANTDGSVTQLIGLSTPTSNPIYPGVFGTMP"
BASE COUNT          739 a         1043 c         1130 g          719 t
ORIGIN      
        1 ttcgcaatcg agacaacgag gcgaaggttg gcttcgataa gctcacgctt ggcgcgctct
       61 gcgtccatgt cgccctggat catctcgcgc tgtgtgcgct tgagatcgac gagcgagata
      121 ccggcgtcgg cttcgacacg gtcgaggtcg atgcggcagt tcttctgctg gcgcttgtac
      181 tccttcttga gctcttcgga gcgcgaggcc tcgaacttcg cctcgagtga acggatctgg
      241 cgctccagcg tgcgcatgga gtcgacggtc ttgttgacct tgtcgagcag ccgcttcttc
      301 tcgccgttgg tgtacttcag ctcgcgaaca atgcggttga cgaacacctg ctcgcggccc
      361 atgagccagc ggctcttgcg cttgtccttc tccttggcct tggcgtcctt gccgtgcggt
      421 tctgcggcgc gggcgccgaa gtcggcggcc ttcttctggt gcttgatcag ctcgtcgatg
      481 cgagagaccg tctgcttcac gcgggcagcg aggatctctt cggtcagctc ttcttcatcg
      541 aatacgacga cttccttaat gttgcggacg ccgcgcttga ggtcttcgcc gagaccggtg
      601 atctcgcgaa tgacgatcgg cgagcgcgag atggccttca tcacgcgcat ctggccgcgc
      661 tcaatgcgct tggcgatttc gacctcgccc tcgcgggtga gcaggggcac ggtgcccatc
      721 tcgcggaggt acatgcggac ggggtcgttg gtcttctcga gcgtgccggg cgaaaggtcg
      781 agttcgacgt cgtcggactc ttcgccggcc tcgtgctcct cgtcgcgctc gccgggcgtg
      841 ccacggcggc catcgctcga gagcacgtcg atgccctggg tgttgatggt ggtcagcaga
      901 tcgtcaagat cgtcgggcga cgtgatatcg ccgggcagaa ggtcgttgac ctcaccaaag
      961 gtgagatatc ccttctcttt cccggtatcg atgatccggt ctacatcttc ttcgtactta
     1021 tcgagctctt cagccacagt tcggcgctcc tcggaaaggc ccacggatgg ggtgggcaaa
     1081 gatcgtgggg aaacctgcgc ttgaaatcct gaggtgcgcg aggaactccc tgaatgtaat
     1141 ggagttatgc cggaaccggg gtgcggtcaa ggccgcgggc gcgggcggaa gtcattggaa
     1201 aagcgatcca gtgcttcggc aactcggagg cgcagggtct ccagcagagc cagtttacat
     1261 cagtttagac gcatttgggc agtcgaaaga tgcagttttc gacccaatta cgctcacgaa
     1321 gcgactcaat taactgcatc cggagtgtca cggcgcccga cccaggagtc gcaggcgacg
     1381 ggctgccaag gtcaagcggg ccgaccgaag ttgtagcgcc cagacacggt ctaggaacag
     1441 gaatctgtac caatttgaaa catagtaagg ggagttttct gaagtttgag acgaacctat
     1501 gaagaaactg ctctccttgc ccctcactcc tattctgctc gccggcgttg cagccggcct
     1561 gctcacaggc tgctccagca gctttgcgcc taacccgatt gaaagtgacc agacgccaat
     1621 cggcaatatc cagggcaggg tgcatggcgg acaatacccg atcagcggag cgcatattta
     1681 cctctatgcg gctggaacga cgggttatgg aaccgccgcg acgtcgctga tctgcaatac
     1741 gggcagcacc tgcggatcca atgcgtatca agacggcagc gggaattact acgtaacgac
     1801 agattcgggc ggaaacttcg cgctgggcgg cgagtatacg tgcacggtcg gcacccaggt
     1861 gtacatggtg gcgacgggtg gggatcaaca attgagctcc gtgcccgcgt acaacaccgc
     1921 gatcgtgcag atggccgcgc tgggccagtg tccaacggcc ggcaatctgg cgcagcaggt
     1981 gccgttcctg accatcaatg aggtgacgac ggtcgccttc gcgtatgcga tgagcggatt
     2041 tgcgacgacg ccctataacg tctcgagcaa cgccgcgaac tcaacggcgt cgaagatcgc
     2101 gattgcgaat gcgatggcca atgccggcaa catcgtgaac ctgcagtggg gccaggcgcc
     2161 ggacaaggcc aacggaaact caaacagcac gaatccgcag acgaagatct atgcgctggc
     2221 gaacatcctc tcgacctgcg tgaatacgac aggcaatacg tccgcgggct gtcaggcgtt
     2281 gttcactgcg gcgaaggact cttcgggaaa cgcgtccacg gacgaggcca gtgcgatctt
     2341 caatatcgcg cataaccagg ggcagaacgt agcggcgatc tggaagctga acccgaagaa
     2401 tccggtcttt tcgccaacgc tgtcgggcac caccggaccg aacgactgga cgttgccggt
     2461 ggtctacacc ggggtgtgca gcgcgttcgg cacgaacggt agcaatcagg tgaccagcgg
     2521 ggcgtataac atcgcgttcg acgtcaacgg gaccgcctgg atcggcgacc gccagaaggg
     2581 cgtgattgaa atgacgccgc tgggagctgt gtcgcacact tacaatccca gcttcagcat
     2641 ggtgaaaggg attgcgatct cgccggattc gacgcagatc tgggttgcgg attactccgg
     2701 cggaacgaac aagacgggcc aggtcgacat catgagcacg gcgggtgcga tcaccacctc
     2761 aatgaccacg gatatcgacg ggccttcttc catcgctctg gatcccaaag gatatgcctt
     2821 tgtggcgaac gaaaccaacg gctcagtggt ggcattctcg tcggcgggcg gtttcattga
     2881 ctacagcggt gcggtgggtg tcactacgcc ggcctggatt tcggtcgacg tgacgggcaa
     2941 tgtatggctg cccagcacga gcggcaacac gacccaggtc gtcgcccagg tcccactcaa
     3001 ctatgccggg aacagcggca agttcaaagg tttcggcacg gctgccacga tcgcggcgga
     3061 ttggtcctac ggtgtggcca tcgattcgaa cgattacgcc tggttcgcca cgaacgacct
     3121 ttccattctg ggaagcacgc cgagtaacga gaatctcgag gacatcgtcc tcaccccaag
     3181 cacgaacaaa aaaggaaaga cgacctattc gtatcagatc tcctcaacgg agagcggctc
     3241 gggaacgaac ggcggcggtc tcgggatccc gtacaagatt tccatcgacg gcggcaacaa
     3301 catttggatg gcgaatgagt actactccac agtgagcggg tacagtgaga cgctgtcgtc
     3361 atggctgatc aagggaacgg cgttcagcaa tggaacaacg gtcggaacgc tttcggcgac
     3421 tccggacaac tccggcaatc tgtggacggc caacacggac ggatcggtaa cccagctgat
     3481 tggattgtcg acgccgacgt cgaacccgat ctacccgggc gtctttggga cgatgcccta
     3541 atcaagtttc gattctggac gaggctgccg ccatcatcgg cagcctttct tttttgtggg
     3601 acgccggagc cggcgcgttg gttagagggg a
//
LOCUS       DATAQL010000318         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3848187, whole genome shotgun sequence.
ACCESSION   DATAQL010000318 DATAQL010000000
VERSION     DATAQL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3848187"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VHS97_05425"
     CDS             complement(<1..554)
                     /locus_tag="VHS97_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447670.1"
                     /translation="MSRQIADEPMPCPGVTRRSFLADTGMGFTGLALGALWAREERAR
                     GSTIETTTPTLVPKAKSVIWIFLCGGVSHVESFDPKPELNRFAGKSIDGTPYKDALNP
                     EKLKDVVAANPMHGGRKVLMGLNTAFRRYGQSGLEVGDWWQNVGSCADDIAVVRSLWT
                     SDNDHGAQLQFHTGRHVREGAHPT"
     gene            complement(568..3243)
                     /locus_tag="VHS97_05430"
     CDS             complement(568..3243)
                     /locus_tag="VHS97_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3447671.1"
                     /translation="MKTPIAIATLAIACLSVAGLPAWARPPHKKALADYLGSAAAKRL
                     NDCRTCHLPPEEGADPQEARPHNPFGKRLKSVRSELKKLAKPSGIPARIEAVADEDSD
                     GDGIANLLELVTGHFPGEADDRPAAAELAKGRAAIAELRRNASGYAWNPFEPVKRPEL
                     PSIRSLGWVKNPIDAFVAAEHEARGLTPRPEANRAMLFRRIYLDLIGLPPTPEQLHSF
                     EADETKNAYDRVVDQLLASPRYGERWGRHWMDVWRYSDWAGWGKQIRDSQPHIWHWRD
                     WIVESLNRDQSYDRMVVSMLAADEAWPEDVSTLRATGYLVRNFKLLSREKWMQDTVDH
                     TAQAFLGLTLGCARCHDHMYEPILQKEYYQVRAIFEPHQVRIDRVPGTLDRSKDGLPR
                     AYDAQLDAKTLLFVRGDDRHPTGEPLEAGVPELLGVPFPKAKPVLLPRAAIAPDRRAF
                     VIGDQFAASQAEVGKARHALAALERESNDSEKLELARIELACADAQLAEHVAVERALA
                     LTEQGLTDSADWKAAAELATKAQRSAAVESARRALALARGARRKAAPPGRAQADKIHA
                     QALKALAQAEQKLRDPLNTEYTAPAATTYPAQSTGRRLALAKWITDPSNPLTARVAVN
                     HLWGRHFDRAIVPSVNDFGRNGQRPSHPALLDWLAAEFMHNGWSMKEIHRLIVTSATY
                     RQDSKPASPELARDPDNIFLWRWTPRRAEAEVVRDCLFAIAGNLESTMGGPDIDHQAG
                     LTVPRRSLYFRHAAEKQMEFLQIFDAAGVTECYRRKDSILPQQALALANSDLSLRQAR
                     RLARTLSSESGSDSASFVIAAFEHVLARAPTEAERIECLGFLGQDAKLVSSTAMESAQ
                     AHESDYKTPASDPALRRRESLVHVLLNHHHFVTIR"
     gene            complement(3308..>3766)
                     /locus_tag="VHS97_05435"
     CDS             complement(3308..>3766)
                     /locus_tag="VHS97_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3447672.1"
                     /translation="MLYDCATEIDSDTVVFHDALTTSLGIPGAAFWEVTEADKVRIFE
                     HKVEPVGEWLIEYADAIVATGGIATHYNLPYGDIYMEVDEPCRRRGFGSYLIQELKKV
                     GYEMGRIPAARCNVANAASRATLQKAGLLPCARILSGVFPEKLEKDPTLD"
BASE COUNT          666 a         1174 c         1171 g          755 t
ORIGIN      
        1 atcgtgggat gcgccccctc gcgcacgtgg cgaccggtgt ggaactgaag ctgggcgcca
       61 tggtcgttgt cggaagtcca aagcgatcgc accaccgcga tgtcgtcggc gcatgagccc
      121 acgttttgcc accagtcgcc gacctcgagg ccgctctggc catatcgtct gaacgccgta
      181 ttgaggccca tcaaaacctt gcgaccgccg tgcatcgggt tcgcggcgac gacgtccttc
      241 agcttctcgg ggttgagagc gtctttgtag ggcgttccat cgatcgactt gcccgcgaat
      301 cgattcaact cgggcttggg gtcaaaactc tcgacgtggc tgaccccgcc gcaaaggaaa
      361 atccagatca ccgacttggc cttgggcacc agagtgggcg ttgtcgtctc gattgtcgag
      421 ccgcgcgctc gctcttctcg cgcccagagc gcccccaatg ccaatcctgt aaatcccatg
      481 cccgtgtccg ccagaaacga ccggcgcgtc acaccagggc agggcatagg ttcgtccgct
      541 atttggcgcg acatcacggg cgtctcctta gcggatcgtg acgaagtggt gatgattcaa
      601 gagcacgtga accaggcttt cgcggcggcg cagggccggg tcgcttgcgg gagttttgta
      661 atccgactcg tgcgcctggg ccgactccat cgctgtcgag ctcacgagct tggcgtcttg
      721 acccagaaaa cccaaacact cgattcgttc ggcttcggtt ggagcccgcg ccagaacatg
      781 ttcgaacgca gcaatgacga agcttgctga atcggagcca gactcgctcg agagcgttcg
      841 cgccagtcgg cgtgcttgac gcagcgacag gtcgctgttg gccagtgcca gggcttgctg
      901 ggggaggatg ctatccttgc ggcggtagca ttcggtcacc ccggcggcgt cgaatatctg
      961 gagaaactcc atctgtttct cggccgcatg ccggaagtaa aggctgcgcc gcggcacggt
     1021 cagaccggct tgatggtcga tatcagggcc acccatggtc gattcaagat tgccggcgat
     1081 ggcaaacagg caatcgcgaa cgacctcggc ctccgcccgc cgcggtgtcc atctccagag
     1141 gaaaatgttg tccgggtcgc gagcgagttc gggcgacgcc ggcttcgagt cctggcgata
     1201 ggtggcactc gtaacgatca ggcggtggat ttccttcatg ctccagccgt tatgcatgaa
     1261 ctcggctgcg agccaatcga gcaaggcggg atgcgacggc cgctggccgt tgcggccgaa
     1321 gtcgttcacc gaaggaacga tcgcacggtc gaaatggcgg ccccagagat gattgaccgc
     1381 gacccgcgca gtcagtgggt tcgatgggtc agtgatccat ttcgccaagg ctagccggcg
     1441 ccccgtgctc tgggcgggat atgtcgtcgc cgcgggtgcg gtatactccg tgttcaaggg
     1501 gtccctgagc ttctgttcgg cttgggcgag agccttaagc gcctgggcat ggattttgtc
     1561 ggcttgagcg cggccgggcg gtgcggcttt ccgccgcgcc ccccgtgcaa gagcaagggc
     1621 gcgtcgagca ctttcaacgg cagcggatcg ctgcgctttc gtcgccagct cggcggccgc
     1681 cttccagtcg gccgaatccg tgagcccctg ttcggtcagc gcaagggccc gctcgacggc
     1741 cacatgttcg gcaagctgag cgtccgcgca cgcaagctcg atccgcgcca attccagctt
     1801 ctcgctatca ttcgattccc gctcgagtgc cgcaagcgca tggcgcgctt tgccgacctc
     1861 cgcctggctc gccgcgaact ggtcaccgat cacaaatgcg cgccggtcgg gagcgatcgc
     1921 cgcgcgaggg agcagcacag gctttgcctt cggaaatggc acgcccaaga gctctgggac
     1981 accggcctcg agcggctcgc ccgtcggatg acgatcgtcg ccccggacaa aaaggagcgt
     2041 tttcgcatca agttgtgcgt catacgcgcg cggcagacca tccttggatc tatcgagcgt
     2101 tccggggaca cggtcgatcc ggacctggtg gggctcgaat atcgcgcgta cctggtaata
     2161 ctccttctgc agaatcggct catacatgtg gtcgtgacag cgggcgcatc caagggtcag
     2221 accgagaaac gcctgggcgg tatggtcgac cgtgtcttgc atccactttt cacggctcag
     2281 aagcttgaaa ttccgcacga gatatcccgt ggcgcgaagg gtactcacat cctcaggcca
     2341 cgcttcgtcg gccgcgagca tcgacacgac catgcgatcg tacgactggt cgcggttgag
     2401 cgactcgacg atccagtcgc gccagtgcca gatatgaggc tgactgtcgc ggatctgctt
     2461 accccaaccc gcccagtcgc tgtaccgcca aacgtccatc cagtgtcgtc cccagcgctc
     2521 accataccgc ggactggcca gaagctggtc gacgactcta tcgtatgcgt tcttagtttc
     2581 gtcggcctcg aaagaatgga gttgttccgg cgtgggtgga agaccaatca aatcgagata
     2641 aatccggcga aagagcattg ctctgtttgc ttcgggccga ggcgtcaagc cccgtgcttc
     2701 gtgttcggcg gcgacgaagg cgtcgatagg gtttttcacc cagcccaagg atcggatcga
     2761 cggcagctct ggacgcttga cgggttcgaa tggattccac gcgtagccgc tggcattccg
     2821 ccggagctcc gcgatcgccg cacgcccttt cgccagttcc gcggccgccg gccgatcatc
     2881 ggcctcaccg gggaagtggc cggtaacgag ctcaagcagg ttggcgatcc catcgccgtc
     2941 gctgtcctcg tcggccacgg cttcgatccg cgccgggatc ccggagggct tggccagttt
     3001 tttaagctcc gagcgcactg acttgagccg cttgccgaac gggttgtgcg gcctggcttc
     3061 ctggggatcg gccccctcct cggggggcaa atgacaggtg cggcaatcgt tgagcctttt
     3121 ggctgcagcg gagccgagat agtcggccag cgccttcttg tgcggtggtc gtgcccacgc
     3181 gggcaatccc gccacgctca ggcacgctat ggccaaagtc gcaattgcga tcggcgtctt
     3241 cattgtccgg gcactcgtgc ttgttgggct cgacaccggt ttggctcacc acaaccgctt
     3301 tcaaagatta gtctaaagtt ggatcttttt ccaacttctc cggaaagaca ccgctgagaa
     3361 tgcgagcgca tggtaacaag cccgccttct ggagtgtggc tcgggaggct gcattagcaa
     3421 cgttgcaacg cgcggccgga atcctgccca tctcgtagcc gaccttcttt agctcctgga
     3481 tcaaatagct gccaaagccc ctccggcggc agggctcgtc aacctccatg taaatgtcgc
     3541 cgtacgggag attgtagtgc gtggcgattc ccccagtcgc gacgatggca tcggcatatt
     3601 caatcagcca ttcgccgacg ggttccacct tgtgttcgaa gatccgcacc ttgtccgcct
     3661 cggtgacctc ccaaaacgcg gctccaggga tgccaaggct tgtcgtcagg gcgtcgtgga
     3721 agacgactgt gtcgctgtcg atctcggtgg cgcagtcgta gagcat
//
LOCUS       DATAQL010000319         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_489987, whole genome shotgun sequence.
ACCESSION   DATAQL010000319 DATAQL010000000
VERSION     DATAQL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489987"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..>2483
                     /locus_tag="VHS97_05440"
     CDS             63..>2483
                     /locus_tag="VHS97_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX3447673.1"
                     /translation="MQFTGLISTGHGPQQPISIKFTDRDSPITRPLVDWTTVNEELYN
                     NAAGKLEATAHELARGKQGSADTAVVWTNIYNGKTRVFGTTLGHNSQTVTDARFLELV
                     TRGLLWSVDKLDDQYLKEVDLLVPEDLSRGKTATASSTQDRDRGPALAVDGDPATRWC
                     ASGPSSPQWWQVDLGKPEDLTGIRILWERGGIEYKYKVEGSDDAKRWTMLSDQTRSPD
                     RDQERLHPFLARGIRYVRVTATGLPPGAWASIFEVAVQGTKRVPAPAATKGAVLPRPL
                     KRAGNDGLLGEVRVPAGFEATLFARPPDVSYPTCLAAAPTGELFVGVDENGSLDARPG
                     RGRVVRCVDVDGDGKADKFNNFAKMDSPRGLVYDAGTMYVLHPPNLTAFHDDDGDGTA
                     DRSETLVKGIGFDLKFRGADHTTNGIRLGIDGYLYVAVGDYGFIKAEGKDGTTLAYRG
                     GGVVRVRTDGTGLEVVSRGQRNIYDVAVDPFLNLFTRDNTNDGDGWDVRLSHVIPTGN
                     LGYPSLFKNFADEIVQPLADYGGGSPTGALYLHEPGFHPPYGDALYTCEWGREAVFRH
                     PLEPKGASFKADQEPFVELPRPTDIDVDGQSRLYIASWRGASFTYAGPNVGYVIRLSE
                     QGVTRPSFPDLKAASDEQLVSYLAASSQVLRLAAQRTILRRGASLLFAQRLEALARSE
                     GPLVPRVAAIFTLEQLLGRDSIETLTSLAAVPPIRQFALHALREHRADAGRVPERPFV
                     DGLTDADPRVRLQAVIGLGRLGKGAGASAIVERTADDDPLVAHVAVQALVALNAVSAC
                     LDALNPATP"
BASE COUNT          457 a          782 c          791 g          453 t
ORIGIN      
        1 tccactgcgc catgcattgc taccgtaccg acggctggaa ccgaaggatc gcgacgccct
       61 ggatgcagtt caccggcctg atttcgaccg gacacggtcc gcaacagccg atcagtatta
      121 aattcaccga tcgggacagc cccataacgc ggcccctggt ggattggacg acagtcaacg
      181 aagagctcta taacaacgca gccggcaaac tcgaggcgac cgctcatgaa ttagcgcgag
      241 gcaagcaggg atcggcagat accgccgtcg tttggaccaa catctacaac ggcaagacga
      301 gggtgttcgg gacaacactg ggccacaaca gccagacggt cactgacgcg cgctttctcg
      361 agctggtcac ccgcggtttg ctctggtccg tcgataaact cgacgatcaa tacttgaaag
      421 aagtcgacct gctcgtaccc gaagatctat caaggggaaa aacggcaacg gcatcatcga
      481 cgcaagatcg cgatcgtggt ccggcgctcg ccgtcgacgg cgatccggcg actcgctggt
      541 gtgcaagcgg gccgtcgagc ccgcaatggt ggcaggtcga tttgggcaaa cccgaagacc
      601 tgacgggcat ccggatcctc tgggagcggg gcgggatcga gtataaatac aaggtcgagg
      661 gttcggacga cgccaaacgg tggacgatgc tttcggacca gaccaggagc ccggaccgcg
      721 accaggagcg gttgcatcct tttctcgcca gaggcatccg atacgtgcgg gtgactgcca
      781 ccgggctccc gcccggcgca tgggcgagca tcttcgaggt cgcggtccag gggaccaagc
      841 gcgttccggc gccggccgcg accaagggcg ccgtccttcc gcggcctctg aagcgggccg
      901 gcaatgacgg tctgctcggt gaggtccgcg tgccggcagg attcgaggcg acgctgtttg
      961 cccggccgcc ggatgtctcg tacccgacct gcctggcggc tgcaccgacc ggcgagctat
     1021 tcgtgggagt cgacgagaat ggctcgctcg acgcccggcc cgggcgcggg cgggtggtcc
     1081 gctgtgtgga cgtagacggc gacggcaagg ccgacaaatt taataacttc gccaagatgg
     1141 atagcccccg cggcctcgtc tacgatgccg gcacaatgta tgtgctgcac ccgccgaacc
     1201 tgactgcttt tcacgacgac gatggcgatg gaacggccga tcgctcagag actctggtca
     1261 aggggatcgg ttttgatctc aagttccgcg gcgccgacca caccaccaac ggtatccggc
     1321 tcggaatcga cggttatctg tatgtcgcgg tgggcgatta cggcttcatc aaggccgagg
     1381 gcaaggacgg cacgacgctc gcctatcgtg gcggcggcgt ggtccgcgtg cgcaccgatg
     1441 gaacgggact ggaggtcgtg tcgcgcggcc agcgcaacat ttacgacgtt gccgtcgatc
     1501 cttttctcaa tcttttcacc cgcgacaata ccaacgacgg cgacggctgg gacgtacggc
     1561 tcagccatgt gattcccaca gggaatcttg gctacccctc gctgttcaag aactttgccg
     1621 atgagatcgt tcagccgctg gccgactacg gcggcgggtc gcccaccgga gcactctatt
     1681 tgcacgagcc cggttttcat ccgccctatg gcgatgcgct ttatacctgc gagtggggcc
     1741 gtgaagcggt ctttcgccat ccgctcgagc ccaagggcgc cagcttcaaa gcggaccagg
     1801 agccgtttgt tgagctgccc aggccgaccg atatcgacgt cgacggccag tcgcggctct
     1861 acattgcctc gtggcgcgga gcttcgttta cctacgccgg tcctaatgtc ggctatgtga
     1921 tccgtttgag cgagcagggc gtgacccgtc cttcctttcc cgacttgaaa gccgccagcg
     1981 atgaacagct cgtctcctat ctcgcagcct ccagccaggt tttgcgcctg gccgcccagc
     2041 gcacgatctt gcggcgcggc gcgagtctat tgtttgcaca gaggctcgaa gcgctggctc
     2101 gatcggaagg ccccctggtg ccgcgggtcg cggcgatttt cacactcgag cagttactcg
     2161 gccgtgattc gatcgaaact ctgacgagcc tggcagccgt gccacctatc cggcagtttg
     2221 cattgcatgc cttgcgcgaa catcgggcgg acgccgggcg cgtgccggaa aggccttttg
     2281 tcgatgggtt gaccgacgcc gatccacggg tgcggctcca agcggtcatc gggctgggcc
     2341 ggctgggcaa aggcgccggt gcctccgcga tcgtcgagcg gacggctgac gacgacccat
     2401 tggttgcgca cgtcgccgtg caggcgcttg tggcattgaa cgccgtcagc gcgtgccttg
     2461 acgcactcaa tcccgcgacc ccg
//
LOCUS       DATAQL010000320         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_31493, whole genome shotgun sequence.
ACCESSION   DATAQL010000320 DATAQL010000000
VERSION     DATAQL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31493"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VHS97_05445"
     CDS             <1..346
                     /locus_tag="VHS97_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447674.1"
                     /translation="NFARRWVVRGGTGINIDTGRTSATDVYFNNLAIGRYLTTKDARV
                     FKELVVHVAVSTLSDVLGRKDYITDVYIAPGVRFGLDRDQKWYALAAIQVPVSGPHPY
                     NWQPNFALVRNY"
     gene            complement(1064..1333)
                     /locus_tag="VHS97_05450"
     CDS             complement(1064..1333)
                     /locus_tag="VHS97_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447675.1"
                     /translation="MCPCQRFAGALAVADAGLGVGMTGWVFPVRLFHSLLSAGFERPT
                     KRTALPADSPDRPASHRVAGDPSDLRVTAADHTLRLIIEPHPPAR"
     gene            1900..>3192
                     /locus_tag="VHS97_05455"
     CDS             1900..>3192
                     /locus_tag="VHS97_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447676.1"
                     /translation="MGDVFHTAGPRTSPQVADQAGDRPADRVSLQHSLVGHLVEAQDS
                     DASLGQPPGIGVAPQDLLGSVHEAPIQPGGPPGAGPIRREIDSVEEPPNGVGRDAQDR
                     REDQPAANRPGAVRAKNPIALAMRRPIKVLILLATLATGAVLGMKKMGVDLPPPLNTP
                     KVHAYLDYVDTGASQIKGFIVRHLGSYFRKQEEEPHHETHKIVITSPRAQDVIITESF
                     VCQIRSQRHIEVRALDSGYLQEISIKEGQAVKKGQSMFKILPVLYEAKYEAEKAEALL
                     AQREFNNTENLYKQKTAVVSQNEVLLFQAKLLRAQANLKKAEAELKFAEVTAPFDGIV
                     DRLLQREGSLIKEGDILTTLSDNGAMWVYFNVPEKYYLEYMAALEQPEKEDKIELVLA
                     NGDKFPQTGTIGAIEAEFNNETGNIKFRADFPNPVNKGT"
BASE COUNT          647 a          983 c         1042 g          520 t
ORIGIN      
        1 gaacttcgcc cggaggtggg tggtgcgcgg gggcacgggg atcaacatcg acaccgggcg
       61 cacgtcggcg accgacgtct acttcaacaa cctggccatc ggccgctacc ttacgaccaa
      121 ggatgcgcgc gtcttcaagg agctggtcgt ccacgtcgcg gtctcgaccc tatcggacgt
      181 cctgggacgg aaagactata tcaccgacgt ctacatcgcg ccgggcgtcc ggttcggtct
      241 ggaccgggac cagaagtggt atgcgctcgc cgccatccag gtgcccgtca gcgggcccca
      301 tccctacaat tggcagccga atttcgccct ggtgcgcaac tattgaccgg gccaatggac
      361 ccgtcgctgt cgcgcttccg ggcggcgagg aggagggccg ggaattggga cgcagtgcgc
      421 cgtgaaaagg tctaggtctt cagatcctga gacaccgtgc atacctccca cgcggtggaa
      481 ttggtgtccg ggacctcatc cggtcgggct agggccgagg taccgagagg agcagggtct
      541 gggaggttca tggcccctac cggccggtgc gattcccgga tcacatcgcg aatcgcagga
      601 aggcgccggt gtctcgcggg cgacgcaacg tgcctcggtc gatcagaccg acgcgcgcaa
      661 cgacttcgtt ggctgcttcg gtcgatggcg cccggtcggc tgaggcagcg gcccggcgac
      721 gacctcatga taggatcacg cacgcatcgc tgtgccatgg gctgactcat cgtggtgagg
      781 actgtcccgc cgccattcgc cacggggctc cccaccgggt gcggccgcgc gctggctgcc
      841 tgtcaccgat tgattctggg aacggcccta tatatcatga acccgggtgc attcccgacg
      901 aggacctcga caccatcaac gctctttgcg ccaagtataa cgtcgaagtc ctcgaatgaa
      961 ccccacacgc cattcggcgt cgtggaggaa tcgagcccgt gaactccgtc gtgcgcgatc
     1021 gagcatggaa cgctttttgc ggggccggaa gaaggccaga agatcatcgg gccggaggat
     1081 ggggctcgat gatgagccgg agggtgtggt cggccgcggt cactcggagg tcggaggggt
     1141 cgccggccac ccggtgcgag gccggccgat ccggactgtc ggctggtagg gccgtgcgct
     1201 tagtgggccg ttcaaaaccg gcggagagta gcgaatgaaa gagtcgtacg ggaaagaccc
     1261 agccagtcat cccgaccccg agtcctgcgt cggcgaccgc aagggcgccg gcgaagcgtt
     1321 gacaggggca cacgtaggcc agccatcgag ctgcgaaatc aggtcctccg gggtgccgac
     1381 gcggttaagc gacgcagaag gcgacaccaa ggacgacgat atgggcgagt cgtccccgga
     1441 ccccgcgcag tcgaagaccc tgtgcatgcg tggaaactct tcgcacggga aacgggagat
     1501 cccacgggta cccaccggtc acacggcggg tcggccggaa aaggtccacg accgtacttc
     1561 cggcatgcac gcccctggga agtcggacga tcgcgtagta cctgagaagc cgccgaacaa
     1621 gggtgtgccc acggcacctg cggaggtggt ggagggaagg cgatcagcca aggggggcac
     1681 gctgacgaag gccgcgttcc ggacgcggag ccggaccgac gcgatgttca ttcgacagcg
     1741 tgcgcgaggc tggctcggtc cagtctccgc caccacccga ggtcggagcc gtaagcggta
     1801 actccgcacg ttcggatctg cgcgggggga cgcctgcaag ggcgttccct accgcgaccg
     1861 ggtgtatagc cggttatctt ccgtccacga ggttatcaca tgggcgatgt attccacacg
     1921 gcaggtccac gaacctcgcc tcaggtagcc gatcaagccg gggatcgccc ggccgatcga
     1981 gtatcgctcc agcactcgct cgttgggcat ctcgtcgagg cacaggactc agatgcctcg
     2041 ctcggccagc cgcccggcat cggcgtagca ccacaggacc ttctcggctc ggtccatgaa
     2101 gcgcccatcc agccgggagg tcctccagga gccggtccga ttcggcggga gatcgactcg
     2161 gtcgaggagc cgccgaatgg tgtggggcga gatgctcagg accggcgtga agatcaaccg
     2221 gccgcgaacc ggccgggcgc agtcagggcc aagaatccca tcgccttagc catgcggcga
     2281 cccatcaaag tcctcatctt gctcgccacg ctggcaactg gtgctgtcct ggggatgaag
     2341 aagatggggg tcgatctccc cccgccgctc aacacgccta aagttcacgc ttatcttgat
     2401 tacgttgaca cgggcgctag tcagatcaaa gggttcatcg tccgccatct tgggtcgtat
     2461 tttcgcaagc aggaggagga gccccaccac gagacccata agatcgtgat caccagcccc
     2521 agggcacagg acgtcatcat taccgagtca tttgtctgcc agatccgctc gcagcggcac
     2581 atcgaggttc gtgccctaga cagcgggtat ctccaggaga tctcaatcaa ggaaggccag
     2641 gcggtgaaga agggccagtc gatgttcaag atcctacccg tcctctacga ggcgaagtac
     2701 gaagccgaga aggccgaggc cctgctggcg caacgggagt tcaataacac cgagaacttg
     2761 tacaagcaaa agacggcggt cgtctcccag aatgaggtac tcctgttcca ggccaaactg
     2821 ctaagggccc aggccaattt gaagaaggcg gaggccgaat tgaagttcgc cgaagtcacc
     2881 gccccgttcg acggcatcgt cgaccgcctg ctccagcggg agggcagcct gatcaaggag
     2941 ggggacatcc tcacgacttt gtccgataac ggcgcgatgt gggtttattt caacgtaccc
     3001 gagaaatact atctcgaata catggccgcc ctggagcagc ctgagaagga ggacaagatc
     3061 gagctcgtgc tggcgaacgg cgacaagttc ccgcagaccg gcacgatcgg tgcgatcgag
     3121 gccgaattca acaacgagac cgggaacatc aaattccgcg cggattttcc gaacccggtc
     3181 aacaagggga cc
//
LOCUS       DATAQL010000321         4856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_642218, whole genome shotgun sequence.
ACCESSION   DATAQL010000321 DATAQL010000000
VERSION     DATAQL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642218"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..2477)
                     /locus_tag="VHS97_05460"
     CDS             complement(261..2477)
                     /locus_tag="VHS97_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3447677.1"
                     /translation="MRYRAYNVMEALRLVGVESDCVDYRLVSTRQSELLAFDLIVLVR
                     RRSSPEIDLLLQFAEKHCIPVICDLDDYLFDEEVLPHSEYLRMLPTEDALSVINEFRG
                     PMLKCRYYTGSTPYLTQRAVSLGVAPYLIRNGLNTTQIELSRIALDAVKAERSGPDLW
                     LGYFSGTLTHQSDFGLIAPVIVRLLEEFPTLGLVVAGDFDLAQFPEFTPFVNRLDQRP
                     FVDWTRLPAEIARVDINLIPLVLSPFTEAKSDLKYYEAAVLKIPSVASPTEVFRACIT
                     HGANGFLASSLDEWHRALRQLITDSALRRLMGQRAYEHVFENYLPEIIAEQAVRVYRD
                     ILMEHRRRLGIDDDAPTFLVLFADLPRAIADRSMALTLCHELARAGARVTVQFDEEPL
                     GYGDDDARGAVTAFLGEDTELTFQVGGEVSCADVVLATDSSTALRVWESRNRSQWPAY
                     LVCEYEPARLAPGLAFDRAVRSFELGLDMLVVDPVVANLLANHRHSRITLLPTRILTE
                     PKGFSGHADPTMVFAVSSSDGVPDTVWTELALALELIKADHPDIRIVLGGQAATRGEL
                     SAMDLPHISQVEGEEFELLLASQPVCVLLCPAGRPPRAPDIMASGCPTIVVNFPDGLP
                     SRDAELTRGVIEVQPKGSEIARAINSLLIDRIHLGSLVLHAAEHVRNLSSPADAARAL
                     IEQFRAVCDTERNAGQQDGPASPDSNHLLVGLRGEREARAHQEPGREPRWTDRRVG"
     gene            complement(3079..4332)
                     /locus_tag="VHS97_05465"
     CDS             complement(3079..4332)
                     /locus_tag="VHS97_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HEX3447678.1"
                     /translation="MLTVTPSVPSAGQRGRGLRLPFGPDRPLFYLHVPKSGGTSVNVL
                     LRRSFPAEQGVRWLEYYPRRLADNSAGLGRHPCYSGHVVYRFRDLLPARTAVMTFLRD
                     PVDRAVSAFYFFRSQGREKLAEEQTTPGLDRCCELSLDEFLEAEPHAARVHLGNIQTW
                     MFSQPHMYHVSPWRPLTLADLDEAKRNLERVAFVGVTARFEESIELLCRMCDWPLPDA
                     VPHENRTPVRPGAEEVSPEARAALEELTTLDADLYRLGAALFADALRDVPLPHVPPAP
                     SRIDLTFEGPIPGSGWFDREPRPGGFFCWTGRAAWLEFRLAGSGELALTVEVRGAVDP
                     AQIDGLTASVNGRPVPMIRSQVGAVARFEGRVATEHVANGRYRVEFRVPRTVRPCDVV
                     SGSTDNRELGVAVSRVELGIAEFAS"
BASE COUNT          991 a         1518 c         1503 g          844 t
ORIGIN      
        1 cttgctgacc accataccag aagcggcggt tgccaacttc atttgaagac ctgtgcctgg
       61 tggctcactg accagccccc ctaacccccc cttcacaagg gggggaaagt tcggcggtgg
      121 cacagaccga ttctgagagg ctacgtccgt agtcattttc ctgccgggcg tcaaggcgaa
      181 ctggacgaac tgcgatgagt ccgcagcgtc gatcactcat attgctcaat gcagttcaag
      241 atgacccaca ctaaggccgt ttacccgact cgtctgtccg tccaacgagg ctcgcgacct
      301 ggttcctgat gcgcccgggc ttcgcgttct ccccgcaatc cgaccagaag atggtttgaa
      361 tccggcgaag ccggcccgtc ctgttgtccg gcgtttcgct cggtgtcgca gaccgcccgg
      421 aactgctcga tcaatgcacg agcggcgtcg gctggactcg atagatttcg cacatgctcc
      481 gccgcgtgca agacaagcga acccaggtga atgcgatcga tcaagagcga gttaatcgcc
      541 cgggcgatct ccgagccctt gggctggact tcgatcactc cgcgtgtcag ttcagcgtcc
      601 ctggacggga gaccgtctgg gaagttgacc acaatggtcg ggcaaccgct cgccatgatg
      661 tcgggcgccc gcgggggtct gccggctgga caaagcaaaa cgcaaacggg ctggctggcg
      721 agaagaagtt cgaattcctc tccctcgacc tgcgaaatgt gcggtaggtc catcgcgctc
      781 aattcgcccc gtgttgcagc ctggccaccc agaacgatcc ggatgtcggg atggtctgcc
      841 ttgatcaact cgagcgccag ggccagctcg gtccagaccg tgtccgggac accgtcacta
      901 ctgctgaccg cgaacaccat ggtcggatcg gcatggccgc taaaaccttt tggctcagtc
      961 aggattcgcg tgggcaatag cgtgatcctc gagtgccggt gatttgccag cagattggcc
     1021 acaacgggat cgacgaccag catgtctaaa cccagctcaa agctgcgaac cgcgcgatcg
     1081 aatgccaagc cgggagccag acgcgcaggc tcgtattcac agaccaagta agccggccac
     1141 tgtgaccggt ttcttgattc ccagacgcgg agcgccgtcg atgaatcggt ggcgaggacg
     1201 acgtcggcac aggagacttc gccgccgacc tgaaaagtca attccgtgtc ctcgcccaga
     1261 aacgcggtga ccgccccgcg cgcatcgtcg tctccatatc caagaggttc ttcatcgaac
     1321 tgaacagtca cgcgggcacc ggctctggcc agctcgtggc agagcgtcag cgccatcgag
     1381 cgatccgcga ttgctcgcgg caagtccgca aacagaacca gaaacgtggg agcatcatcg
     1441 tcgattccga gccgtcggcg gtgctccatc aagatatcac gataaacgcg caccgcctgc
     1501 tcggcgatga tttctggcag ataattctcg aacacgtgct cataagctct ttggcccatc
     1561 agccggcgca gggcagaatc cgtgatcagt tgtcgaagag ccctgtgcca ctcgtcgagc
     1621 gatgacgcca ggaaaccgtt ggccccgtgg gtgatgcacg cacgaaagac ctctgtcggc
     1681 gaagccacgc tggggatctt gagcacggct gcttcgtagt atttcaggtc gctctttgcc
     1741 tcggtaaacg gactcaggac cagggggatc aagttgatgt cgacacgggc aatttccgcg
     1801 ggaagccgcg tccaatcaac gaacggccgt tgatcgaggc ggttgacaaa gggcgtgaat
     1861 tccggaaatt gagccaggtc gaaatccccc gcaaccacaa gtccaagcgt ggggaactct
     1921 tcgagcaggc gaacaataac cggtgcaatc aaaccgaagt ccgattgatg tgtcaatgtt
     1981 ccgctaaaat agccgagcca cagatccggc ccgctccttt cggctttgac cgcgtcgaga
     2041 gcgatcctgg acagttcgat ttgagtcgtg ttcagtccgt tgcgaatcag gtaaggggcg
     2101 actcccagcg aaactgcccg ttgtgtcaag tacggcgtcg agcccgtgta gtaacggcat
     2161 ttcaacatcg gcccgcgaaa ctcgttgatc accgacagcg cgtcctcggt cggcagcatt
     2221 cgcaggtatt cgctgtgtgg caagacctct tcgtcaaaca ggtagtcatc gaggtcgcag
     2281 atgaccggaa tgcagtgctt ttccgcaaac tggaggagca ggtcgatctc cggtgagctg
     2341 cgccggcgga ccagcacgat gagatcaaac gcgaggagct cgctctgacg agtggagacc
     2401 agtcgataat cgacgcaatc ggactcgacc ccaacgagac gcagtgcttc catgacgttg
     2461 taggcgcggt accgcatgca ggaagcatcc caaataccgg gaatcacgac aaagacaacc
     2521 cgaaagcaat gcggagatgc aatgcgcggg ccgccagtcc tctccacacg gcggcgtaag
     2581 agtgtgggca aggggggcgg aggctgttga gtctgctgaa caggcgcggg agctttttgc
     2641 agaagtctca aactgcgccg aatcacgccc cggacgaccc gacggccttc cgatttcagc
     2701 gatctacctg ccgcggtata caattcagac agcgacatgg ccgttctttc tcagcggatg
     2761 ggaacttgtc catcgaagcc ttgggcgatt cgtccaccgc cggacgatcg ctcaccgctt
     2821 gaagacaacg caaatgtttc cgtaaaaatg caggtgatca atctggaatc gaccgccgat
     2881 cccggaccaa tacgcgggaa tatcgccggc gtcttgcgcg ctgtgaaagt ggtcccaaga
     2941 gaacggaaca ctggcgtcta tgaatgccgg gggatcgtag tcatcggaat cgtcccggtc
     3001 aacatgactt ggcgagcagt gacgagtcga atatttaagt gcgcgtatcc gggctcggaa
     3061 catcagcggg ctcgtgcatt aggaggcgaa ctctgcgatt ccgagctcga ccctggaaac
     3121 ggccacgccc agctcccggt tgtcggtgct cccggacacg acgtcacacg gccggacggt
     3181 ccggggcacc cggaactcaa ctcgatagcg gccgttggcg acatgctccg tcgccacccg
     3241 cccctcgaac cgggcgaccg ccccgacctg ggatcggatc atgggcaccg gccgcccgtt
     3301 gacagaggcc gtcagaccgt cgatctgggc cgggtcgacg gcccctcgga cttcgaccgt
     3361 cagggccagc tccccgctcc cggccagccg gaactccagc caggcggccc gcccggtcca
     3421 gcagaagaac ccgcccgggc gcggctcccg gtcgaaccac ccggacccag ggatcgggcc
     3481 ctcgaaggtc aggtcgatcc gggacggggc cggcgggacg tgcggcagcg ggacatctcg
     3541 cagcgcatcg gcgaacaagg ccgccccaag ccggtacagg tcggcgtcca gcgttgtcag
     3601 ctcctccagg gcggcccggg cctcggggct gacctcctcc gctcccgggc gaacgggggt
     3661 gcggttctcg tgcgggacgg cgtccgggag cggccagtcg cacatccggc acaagagctc
     3721 gatcgactcc tcgaaccggg ctgtcacacc tacgaacgcg acccgttcca ggttccgctt
     3781 ggcctcgtcg aggtccgcca gggtcagcgg ccgccagggc gagacgtgat acatgtgcgg
     3841 ctgggaaaac atccacgtct ggatgttccc caggtggacc cgcgcagcgt gcggttcggc
     3901 ctccaggaat tcgtccagcg acagctcgca gcaccggtcc aagcccgggg tcgtctgctc
     3961 ctcggccagc ttctcccggc cctgggatcg aaagaagtag aaggccgata cggcccggtc
     4021 gaccgggtcc cggagaaagg tcatcacggc ggttcgcgcg ggcagcaggt ctcggaaccg
     4081 gtagacgacg tggccggagt agcaggggtg ccggccgagc ccggccgagt tgtcagccag
     4141 ccgccgaggg tagtactcga gccaacgaac tccctgctcc gctgggaacg accggcgcag
     4201 caggacgttg accgatgttc cccccgactt cgggacgtgc aggtagaaaa gcggccggtc
     4261 gggtccgaac ggcagtctga gcccgcggcc tctctgtccg gccgatggga cactcggggt
     4321 cacggtgagc atgcaaaccc ccttttcgga tacacgatag caggtcgctc cggtccatac
     4381 cggcgcggtg gctgcgggat ggacccgcga ccgaaacgcc gccgaggatg ggccatcgtc
     4441 cgggaggctc ctctggcacc agaggggagg cgagtcagcc aagtcgccga cgaatgaacc
     4501 ctttatcggc agattgtgtc ggatagctcc gaagaaaacg tgccaggtat gtgataagag
     4561 tgggccggtt ggtcaagccg actcaccgcg tcgcatgctc gatcggaccg gacattttcg
     4621 gtcagttcga aggacaaagg cctgtgacaa cggaggcacc atgtcgagca gatccggatc
     4681 tgtgggagcc ccaggagagc aatttcctcg ggccacccgg cccatttatc cttcccttta
     4741 tcacttcctg ttggcgaatt cggcccaggc gtaatgccag cacgcatgca gattaggtcg
     4801 ggcgatcggg cggaccgagg cggtcccgat cgtacacggc ccacggacat caccca
//
LOCUS       DATAQL010000322         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2473896, whole genome shotgun sequence.
ACCESSION   DATAQL010000322 DATAQL010000000
VERSION     DATAQL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473896"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHS97_05470"
     CDS             <1..202
                     /locus_tag="VHS97_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447679.1"
                     /translation="ANGSDDKRFNMEPSAPVKTNGHDSIFVREGELMSKLHDLLHSPM
                     GDGIARILEEMHALIPRSRSNG"
     gene            340..843
                     /locus_tag="VHS97_05475"
     CDS             340..843
                     /locus_tag="VHS97_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447680.1"
                     /translation="MKRIQMAAARVVVFTLVALPTFTPSGFTRAGEPPRRWGRPVVVT
                     RARPRPAPSSSTTLGTFQPTPTIWIGGSYPTGRGYSPMNIYGDQTLTLYGPLSAFRVS
                     TAPILGYTRGYDGQLRVTEANSFSYPNLPDLSPVVYPTEANYFYGPRVNKTPRWGSNA
                     INWIDQN"
     gene            896..2575
                     /locus_tag="VHS97_05480"
     CDS             896..2575
                     /locus_tag="VHS97_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX3447681.1"
                     /translation="MHRSLGQILVDSGQAEEALPHCQEAVRLDPNSAVLHHNLGNALR
                     ALEKYVDAKAEYLEALRLNPKLAVANCHLGLVLQREGQLADALVWIKKGVELEPGNAD
                     IWEWLAELYDEMEEPVESIPCWERVITLEPDRAGAHLSLGWALQEEGKLALARQHYDK
                     AIQLKPDLAGAYLNLGGLEEEIGDLAGAEQAFRTALKLQPTFALPHARLATLLRTKLP
                     NEDLAALEERLTDDKLDEGPRGRLLFALAQALDGLGDHTRAAQCLREANALAIESDRK
                     RRDYDPVEHERFIDGLLSGFDRGLFARLATAGVPSRRPVFVFGLPRSGTTLIEQVLAS
                     HSQIHGAGELRLARRSFEAIPSLLGCSDSPRECITALDRQTALKLAKLHLENLNEYDG
                     RRNPRIVDKLPDNYMYVGLLAIMFPRAAFIHSRRDLRDIAVSCWMTDFRNIRWANDQS
                     HIAKRFQQYHRVMNHWKAVLPIPIHEVHYEETVTHLETVARRLIDVCGLPWEPACLEF
                     HRAERPVRTASVLQVRQPVYQRSVARWKHYEPHLGELFAQLPQEQEPSSRS"
BASE COUNT          522 a          824 c          804 g          541 t
ORIGIN      
        1 ggccaacgga tcggatgata agcgcttcaa tatggagcca tcagcgcccg taaagaccaa
       61 tggtcatgac tcgatcttcg tccgtgaggg agagttgatg tcaaagctgc acgacctgct
      121 acattccccg atgggcgacg ggatcgctcg cattctcgaa gagatgcatg cgctgattcc
      181 gcggtcgcgt tcgaacggct aatgggttta ttcggcgcac gctgtggatt tatcgggtag
      241 atttcctcaa ggaaccgatt cgcgttgacg atgacgaacc gattccaagc gggtcgtgct
      301 ctgtcggtgc gatcagccgc taaccttgag gaacatccga tgaagcgcat ccaaatggcc
      361 gccgcgcgcg ttgttgtctt cacacttgtt gccttgccga cgttcactcc atctggcttt
      421 acgcgagcgg gtgaaccgcc gcggcgctgg gggcgcccgg tggtggtcac gcgggcgcgt
      481 ccgcgccccg caccctccag cagcacgacc ctcgggactt ttcagccgac accgaccatc
      541 tggatcggag gcagttaccc gaccggccga ggttattcgc cgatgaacat ctatggcgac
      601 cagaccctga ctctttacgg gccgttgtcc gccttccggg tgtcgacagc gcctattctc
      661 ggctacactc gcggctacga cggccagctt cgcgtcaccg aggcaaactc gttctcgtac
      721 ccgaatctac ccgatttgtc gcccgttgtt tatccgaccg aggctaacta cttctacggg
      781 ccgcgagtga acaagacgcc tcgatggggc tcaaacgcga tcaactggat cgaccagaac
      841 tgacttttga acgggttgct ctcgtatgca agatgccctg acgaccgcca tcgcaatgca
      901 tcgatccttg ggccagattt tggtggacag tggccaggcc gaggaagccc tgccgcactg
      961 ccaggaagcc gttcgccttg accccaattc ggccgtactg caccacaacc tgggcaatgc
     1021 gctacgtgcg ctcgaaaagt atgtcgacgc caaggcagag tacctcgaag cgttgcggct
     1081 gaatccgaag ctcgcggtgg ccaactgcca cctggggctg gtattgcagc gcgaggggca
     1141 gttagccgac gcgcttgttt ggatcaaaaa aggggttgag ctcgagcccg gcaacgcgga
     1201 catctgggag tggttggctg agttgtacga cgagatggaa gagccggttg agtcgattcc
     1261 ctgctgggag cgggtgatca cgctcgagcc cgatcgcgcg ggtgcgcatc tctccctggg
     1321 atgggccctg caggaggaag gtaagctcgc cctggcccgc cagcattatg ataaggccat
     1381 ccagttgaag cccgacctcg ccggcgccta tctgaacctg ggcggactag aagaggaaat
     1441 cggcgatctg gcgggggccg aacaggcttt ccggacggcg ctcaagctgc agccgacctt
     1501 tgcgctgcct catgcccgcc tggcgacgct cttgcgaacc aagctgccga acgaagacct
     1561 ggcggccctc gaggaacggt tgaccgacga taagctggac gagggcccgc gcggccggct
     1621 cttgtttgcc ctggctcaag cactcgatgg cctgggcgat cacactcggg ccgcccaatg
     1681 ccttcgcgag gccaacgcgc tggcgatcga gtctgatcgc aagcgccgcg attacgatcc
     1741 cgttgaacac gaacgcttca tcgacggtct cttaagcgga ttcgatcgtg gcttgtttgc
     1801 gcggctcgcg accgctggcg tgccaagccg gcggcccgtt ttcgtctttg gcttgcctcg
     1861 ctcgggcacg acgttgattg aacaggtgct cgccagccat tctcaaatcc atggggcggg
     1921 tgaactccgg ctggcccggc ggtcgtttga ggcgattcct agtttattgg gctgctccga
     1981 ttctccgcgc gaatgcatta ctgcgctcga tcgacagacc gccctaaagc tggccaagct
     2041 ccaccttgaa aacttgaatg agtacgacgg ccgacgcaat ccgcggatcg tcgataagtt
     2101 gcccgacaac tacatgtacg tcggcctgct tgccatcatg ttcccgagag cggctttcat
     2161 acatagccgg cgcgacctgc gcgacatcgc cgtatcctgc tggatgactg acttccgcaa
     2221 cattcgttgg gccaacgacc agagccatat cgccaagcgg ttccagcagt accatcgcgt
     2281 gatgaatcac tggaaagcag tgctgccgat tcccatacac gaggtgcatt acgaagagac
     2341 cgtgacccac ctcgagacag tggcgcggcg gctgatcgat gtgtgcggcc ttccctggga
     2401 gccggcctgt cttgagtttc accgcgctga gcgaccggtg cgcacggcca gcgtcctgca
     2461 agtccgccaa ccggtttatc agcgatcggt cgctcgctgg aaacactacg agccgcattt
     2521 gggcgagctt ttcgcgcaat tgccccaaga acaggagcct tcttcacggt cttgatcaag
     2581 ttgacggcca ctgactcatg ggatcaccga gaagagctca tccaggtcgg ggacgacctc
     2641 gccatgttcg agacgatcgg ccaaagctcg gagacataaa gctttcacct t
//
LOCUS       DATAQL010000323         5791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3389573, whole genome shotgun sequence.
ACCESSION   DATAQL010000323 DATAQL010000000
VERSION     DATAQL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3389573"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..2120
                     /locus_tag="VHS97_05485"
     CDS             366..2120
                     /locus_tag="VHS97_05485"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX3447682.1"
                     /translation="MAEQPNRGFYVAVGIVVLALIGFAVYRSDLVAPKPAPPPGGGGE
                     GAQAKIDPSEVKQEAESGQDTSTPTTVKEYRFKPAEKLPPVKGTAAYKPLVNNTVRFA
                     LNVWAGWAPIVLANNGFRAGTDWTASDGQKFKVELVLIDNTVAMRDAYAAGEVPIGWA
                     TLDMVPLFLESFVDSQGNPRDSRIMPRIYQQVDFSNGGDGIVVREDVKTAADLRGKKV
                     VLAQNSPSHYFALNMLVAGGVQPPEVQMVFTEDAFQAAAAFNSQKDITGCVSWAPDIY
                     NLSKLKGNRMLVTTQDANRLIADVWFARADFARDHGPILEGLARGIFDGMERLKDQTE
                     KKKVSELMAAGYNIPASDALSMLGDAHSTNWAENFQFFINENNPTNFERIWQRAYLLY
                     RRIGSISHRPVPFDQVMDFSIVQKLGQDEKYSSSKVEYETKVAPKELQAVRAEGDEIL
                     TNTVVIHFFPNSWDPFNTVNKTINGKAVQERYDPNVENVLEEIAQLAGQFGTARIIIE
                     GHTDSSMKGQVPVEAVKDLSLKRAAAIKEALLKQYTELDANRFNVDGVGWDRPVDPLN
                     QTKNRRVEIKVLTAEKAN"
     gene            2307..3332
                     /locus_tag="VHS97_05490"
     CDS             2307..3332
                     /locus_tag="VHS97_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3447683.1"
                     /translation="MTSSTGSPAATPGLLPQGASPASRRWLLDLRFALRRGLPAWQAA
                     FMGLVGVFVCLALWWFLTNGPTEERIIPPSKGLSSPAETFASFHSLWFDRALTRNLLT
                     TLKRLALGFGLAALVGVPLGVFCGCFPRVQAFFLPVTLFGRNIPVAALIPLTFSLFGI
                     GELQKIMFIFIACVAFIVWDTTRAIEEVGSQYIDTAFTLGAGTWQTIGKVLLPLALPS
                     VFNSLRLIFGLAFGYIMLAEVIKFGGETGGLGDLINISQRRGPREHVFLILLIIPVVA
                     LLIDRLFFVIQAELFPYRYGGMGILNRLIRAVLHAWEDLRLAVWKALFGPKIKRSHAN
                     PADPQAP"
     gene            3329..4249
                     /locus_tag="VHS97_05495"
     CDS             3329..4249
                     /locus_tag="VHS97_05495"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3447684.1"
                     /translation="MSATEQEPTTPANTPPSQQVGIGRVLVGTRRPDEVREPHVVEFH
                     GVSKTYNRGRPNEFTAIRDVNFVVQDLIDKGEFICILGPSGCGKSTILRLIAGLRPQH
                     PPTSGKLLVMGQPVAGPGADRGMVFQDYTSFDHRTVLDNVSFGLECQGEPRQDRYEHA
                     RRWIARVGLDVEKDQDKYPHELSGGMRQRVAIARTLILHPRIILMDEPFGALDPMTRM
                     TMQDLLLELWRTTQATVFFVTHSIEEAVFLGDRIYVLSNSPGTILHELSIKPSDRPSK
                     EMQREEKFQETVYYLRDLISRLEESRRAGT"
     gene            4289..5209
                     /locus_tag="VHS97_05500"
     CDS             4289..5209
                     /locus_tag="VHS97_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447685.1"
                     /translation="MGLGKYIKKAFVYQWNLLAFLGGMGFAVLSGHPDVVIPLVLAGE
                     TAYLGFLGTHPRFQKFVDAQDHKTARAQADAEAVERLKNGLPPAQLRRFQALRDRCLA
                     LRQIAEQLREPAGVDSLRSLDELQLSDLDRLLWIYLRMLYTQHMLEQFFEKTSADQIT
                     DEIRRLENRIRKLEKEPDSANRSRIRQSLEANLDTCRSRLSNLDKARENHELLQAEIE
                     NLETKIQSITELAINRSDSEAITGQVEQITQGLIRTEQTINDLGFATGVEKFDLTVPR
                     ILARDLGGPTDDAREAPSSRRQRENGIHYQ"
     gene            5235..>5791
                     /locus_tag="VHS97_05505"
     CDS             5235..>5791
                     /locus_tag="VHS97_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3447686.1"
                     /translation="MALPRGRLILGINSAYHESAAAIVRDGEVVCAIEEERFTRIKHA
                     KTASVFNPDLLPWNAIRACLQAVPGATLRDLNGIGYSLEPGRRLALIGGDPHEIDDET
                     GFGSRAGEEEFNTRVMSIPYVLASAAGDDSLAASFHFVPHHLAHAASAFYAFPFSSAA
                     VLVVDGIGETSTAWLGRGSPQGLVRL"
BASE COUNT         1187 a         1791 c         1661 g         1152 t
ORIGIN      
        1 tccgctgttt ccgctgaggc atcgaagatt gacgatttgc ggccgagcga tgtccggctc
       61 agggcagggt aagttggcac tggaaacctt cgcagaagtg tcaaatagtg ccaaaaagta
      121 ccactcgttt tgtgacgtaa ctttgcaggc ccgccatcgc gaccgtcccc gtcaatttcg
      181 tcatctgcga gacaaatctg aacttgcaag ccacgcctca ataaagcgct cgttccggcg
      241 attcaccttg gggcggtcca ttgaacggcc gggagacgag gggtactctc gtaccaaagt
      301 agccctcaga ttgacctgag gcgaagaaat ctggccggcg aaccggaccg acggagactc
      361 gaaacatggc cgaacaacca aatcgtggtt tctatgtagc cgtcggcatt gttgtccttg
      421 cgctaatcgg gtttgcggtt tatcgctctg acttggtcgc tcccaagccg gccccaccgc
      481 cgggcggtgg aggcgaaggt gcccaggcga aaatcgaccc gagcgaggtc aagcaagagg
      541 ccgagtcggg acaggatacg tcgacaccga ccaccgtcaa ggaataccgt ttcaagcccg
      601 ccgaaaagtt gcccccggtc aaagggacgg ccgcttacaa gccactcgta aacaacacgg
      661 tccgattcgc gcttaacgtc tgggcaggtt gggccccgat cgtactggcg aataacggtt
      721 tccgggccgg aaccgattgg accgccagcg atgggcagaa gttcaaggtc gagctggtct
      781 tgatcgacaa cacggttgcg atgcgcgatg cctatgcggc cggcgaagtc ccgatcggct
      841 gggccactct cgatatggta ccgctattcc ttgaaagttt tgtcgactcg cagggcaacc
      901 ctcgtgatag ccggatcatg ccgcgcatct atcagcaagt cgatttttcc aacggcggcg
      961 acggcatcgt cgtgcgtgag gacgtcaaga ctgccgccga tctgcggggg aagaaagtgg
     1021 tgctggcgca aaactcgcct tcgcactatt tcgcgcttaa catgctcgtc gccggtggcg
     1081 ttcagccccc tgaggtccaa atggttttca cggaagatgc atttcaggcc gcggccgcct
     1141 tcaattcaca aaaggacatc accggctgcg tctcgtgggc tcctgacatc tacaacctgt
     1201 cgaagctcaa gggcaaccgc atgctggtga ccacccagga cgccaaccgg ctgatcgccg
     1261 acgtctggtt tgcccgcgcc gactttgccc gtgaccacgg tccgatcctc gaaggcttgg
     1321 cacgcggaat cttcgatggg atggagcgac tcaaagacca aacggagaaa aagaaggtct
     1381 ccgagctcat ggccgccggc tacaacattc ccgcatccga tgcgctctcg atgctgggcg
     1441 atgcccacag caccaactgg gctgaaaact tccagttctt catcaatgag aacaacccga
     1501 ccaacttcga gcgaatctgg cagcgagcct atctgctcta tcgccggatc ggttcgatca
     1561 gtcatcgtcc cgtgccgttc gaccaggtca tggatttctc gatcgtccaa aagcttggcc
     1621 aggatgaaaa gtattcttcg tcgaaggtcg agtacgagac gaaagtcgct cccaaggaac
     1681 tgcaggccgt gcgtgcggaa ggggatgaaa tcctcaccaa cacggtcgtg atccatttct
     1741 tccccaatag ctgggatcct ttcaataccg tcaataaaac gatcaacggc aaagcggtcc
     1801 aggagcgcta cgatccgaat gtcgaaaacg tgctcgaaga gatcgcccaa cttgccggtc
     1861 agtttggtac cgcgcggatc attatcgaag gccacaccga cagctcgatg aagggccagg
     1921 tccccgtcga ggcggtcaag gatctgtcgc tcaagcgggc cgccgccatc aaggaggcat
     1981 tgctcaagca gtacaccgag cttgatgcca atcgattcaa cgtcgatggc gtggggtggg
     2041 accggccggt cgacccgctc aaccagacca agaaccgacg tgtcgagatc aaggtgctca
     2101 ccgcggaaaa ggcgaattga tttaacctgg ctgctctcga gtccgctact ctaactgccc
     2161 ttgcacgatc cgggagggcg ggcctcccgg cgagccacgg cgcaatccgg ctcggacgga
     2221 gcctcgccct cccggaatca cgctagggcg tttaggaaaa gctcaccggc tccgcgagca
     2281 gcaccatcac cgaatgagat cttggcatga catcctcgac cggatcccct gccgctaccc
     2341 ccggcttgct tccgcagggc gcgagcccag caagccgcag gtggctcttg gatttgcgat
     2401 tcgccctccg ccgcgggctg cccgcctggc aggctgcgtt catggggctc gttggcgtgt
     2461 tcgtttgcct cgcactctgg tggtttctca ccaatggccc gaccgaagag cgaatcatac
     2521 cgccgtcaaa gggcctttcg agcccggctg aaacatttgc gagctttcac agtctctggt
     2581 ttgaccgggc gttgacccgc aaccttttga ccacgctcaa gcgcctggcg ctgggcttcg
     2641 ggcttgcggc tctcgtcggt gtaccgctgg gtgtgttctg cggctgtttt ccgcgggtgc
     2701 aagcgttttt cctgcccgtg acgttgttcg gccgcaacat ccccgtggca gcgctcattc
     2761 cgctaacgtt ttcgctattc ggcatcggcg aactgcaaaa gattatgttt atattcatcg
     2821 cctgcgtggc gtttatcgtc tgggatacga cgcgtgccat cgaagaagta ggcagccagt
     2881 acatcgacac ggcgttcacc ttgggcgccg gcacatggca gaccatcggc aaggtgctcc
     2941 tccctctcgc gttaccaagt gtgttcaact cgctgcggtt gatcttcggg ctggcattcg
     3001 gctacatcat gctggccgag gtgatcaaat tcggtggcga aaccggcggc ctgggcgatc
     3061 tgatcaacat ctcgcagcgc cgcggtcccc gcgaacatgt gttcttgatc ttgttgatca
     3121 tcccggtggt tgcgctcttg atcgaccggc tttttttcgt gattcaggct gagttgttcc
     3181 cgtaccgcta cggcggcatg ggcatactga accgtctgat cagagcagtg ctccacgcct
     3241 gggaggacct gcggctggcg gtttggaagg cgctttttgg gcccaagatc aagcgctccc
     3301 acgctaaccc cgccgatccc caagcaccat gagcgccacc gaacaagagc ccaccacgcc
     3361 cgcgaacaca ccaccgagcc agcaggtcgg catcgggcgg gtactcgtgg gaacccggcg
     3421 acccgacgaa gtacgtgaac cgcacgtcgt ggagtttcac ggcgtttcca agacctacaa
     3481 tcgggggcga cccaatgaat tcaccgcgat tcgcgacgtc aacttcgtcg ttcaagacct
     3541 gatcgacaag ggggagttca tctgcatcct tggtccgagc ggctgcggca agagcacaat
     3601 cctgcgactg atcgccggcc tgcggcccca gcatcctccc acgagtggga agctactcgt
     3661 gatgggccag ccggtcgccg ggccaggcgc cgatcgcggc atggtctttc aagattacac
     3721 gtcgtttgac caccgcaccg tgctcgacaa tgtctctttc ggactggaat gccagggcga
     3781 gccccgccag gatcgatacg agcatgcccg ccgctggatc gctcgggtcg gtctcgatgt
     3841 cgaaaaagac caggacaagt acccccacga gctctccggt ggaatgcgcc agcgggtcgc
     3901 cattgcgcgc accctgatcc tacatccccg aatcattttg atggacgagc ctttcggcgc
     3961 gctcgacccg atgacccgca tgaccatgca agatctcctg ctcgagctct ggcggacgac
     4021 ccaggcaacc gttttctttg tcacgcattc gatcgaggag gccgtcttcc tgggggatcg
     4081 catctatgtt ctctccaact cccccggcac aatactgcac gaactgagca tcaagccatc
     4141 ggaccggcca tccaaggaga tgcaaaggga ggagaagttt caggaaacgg tgtattatct
     4201 ccgtgatctg atcagcaggc tcgaagagag tcggcgagcc ggcacctaat tagaatgacc
     4261 gatcaacctg ggaaggattg cgaccgtcgt gggtctggga aaatacatca agaaggcgtt
     4321 cgtttatcaa tggaacctgc tcgcattttt ggggggcatg ggattcgccg tgctttccgg
     4381 gcaccccgat gtcgtgatcc cgctggtgct ggccggcgag accgcttatc tgggctttct
     4441 cggaacgcat cctcgttttc agaaattcgt cgacgctcag gatcacaaga ccgcccgagc
     4501 ccaggcagac gccgaggccg tcgaacgctt gaagaacgga cttccccccg cgcaactccg
     4561 tcggtttcag gcgctccgcg accggtgcct ggccctgcgc cagattgccg agcagttgcg
     4621 agagccagcc ggtgtcgatt cactccgctc gctcgacgag ctgcaactct ccgatctcga
     4681 ccgcttgctg tggatctatc tacgtatgct ctacacacag cacatgctcg agcaattctt
     4741 cgagaaaacc agcgccgacc agatcaccga cgagattcgc cggctcgaga accggatccg
     4801 taagctggaa aaggagcccg attccgccaa ccgctcccgg atccggcagt ccctggaggc
     4861 caacctcgat acgtgccgtt cccggctctc caacctcgac aaagcgcgcg agaatcacga
     4921 attgctgcag gccgaaatcg aaaacctcga aaccaagatc cagtcgatta ccgagctggc
     4981 gatcaaccgt agcgattccg aagctatcac cggtcaggtc gaacagatca cccagggctt
     5041 gatccgcacc gagcagacga tcaacgacct cgggttcgcc actggcgtcg agaaatttga
     5101 tctgaccgtt cccagaatcc tcgcgcgcga cttgggcggt ccgaccgacg atgctcggga
     5161 ggcgccctct tcacgccgtc agcgcgagaa cgggatccat taccagtaaa tgccgctctt
     5221 gttaaggtcg cgtcatggct ctcccccgag ggcgtctcat tctggggatc aactccgcgt
     5281 accacgagag tgccgcggcg atcgttcgcg acggcgaagt tgtctgcgcg atcgaggagg
     5341 aacgattcac acgaatcaag catgcgaaga ccgcatccgt tttcaatccc gacctactcc
     5401 cgtggaacgc catccgcgcg tgccttcagg cggttcccgg agccacgctc cgagatctga
     5461 acgggatcgg ctactctttg gaaccaggca gacgactggc cttgatcggc ggcgatcccc
     5521 atgagatcga cgacgagacc ggtttcggga gccgtgcggg cgaagaagaa ttcaacacac
     5581 gtgtcatgag catcccatat gttctggcga gcgccgccgg tgacgactcg cttgcggcaa
     5641 gtttccactt cgtaccccat catcttgccc acgcggcgag cgcgttctat gcgttcccgt
     5701 ttagctccgc ggcggttctt gtggtcgatg ggatcggcga gacgtcaacc gcatggcttg
     5761 gtcgcggctc gccacagggt ctcgttagac t
//
LOCUS       DATAQL010000324         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4000782, whole genome shotgun sequence.
ACCESSION   DATAQL010000324 DATAQL010000000
VERSION     DATAQL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000782"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..1152
                     /locus_tag="VHS97_05510"
     CDS             502..1152
                     /locus_tag="VHS97_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX3447687.1"
                     /translation="MRTDDLYDFPELSFRYGLMDRVELRTLWIGPTFDEESAASRRAR
                     RGIGGAADMEVGIKWQLFAGDKERKWIPTTALITSAIAPTGGSSPLSSGVAEPYINLI
                     YGWHPNDKLALCGSTGYLGQRQQAVAGSGLPADSFQRFHQSLVAFYSVGERTTVFYEW
                     YVLMFTNAPNNLPTHFMDGGLLYRLTPNIQVDLRAGFGLSGRPEDVFAGTGFSFRF"
     gene            complement(1210..2556)
                     /locus_tag="VHS97_05515"
     CDS             complement(1210..2556)
                     /locus_tag="VHS97_05515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX3447688.1"
                     /translation="MDPTFIDRALGSNVPYITYAVPFFFLLIGVELVVALWQREHSYR
                     LHDSINDLSCGITEQVVGIFLKGLLFAGYLRTYDFATHAGFNWIDVQAYSTGGKWVAA
                     IVLFLGVDCAYYWFHRIAHEYNAPWAGHVVHHSSEDYNLAVALRQGTFQGFFSWVFYL
                     PLAFVGFPPPWFAAMLSFDLLYQFWINTRAIGKLGPLEWVLNTPSHHRVHHARNPKYL
                     DKNYAGTLIIWDRMFGTFQAEEEEPVYGLTKPLNSWNPLWANLHVWSELCCDAWLAPR
                     WIDKLRIWFMPQGWRPEGLPANPTAPEVSRATVVLYDTRVPRGLDAYAIVQFVAALLL
                     AIGLLAVGESLSRAQLIIAALLVLWTLLSVGGIFDQRPWALISELVRLPVTAIVVAAS
                     LPDFTSIVPIGLALMVLAVWLFLLSQRRHFVGAPQHQSPKNASASSPRYSARVLGR"
     gene            2881..3096
                     /locus_tag="VHS97_05520"
     CDS             2881..3096
                     /locus_tag="VHS97_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447689.1"
                     /translation="MRRTPPTTEPFSRNSLDATFVWMLADQKERQCAADVQRPRDVLR
                     SEIEYYVFALRELAERKHRPLVRIGSG"
     gene            3328..>3558
                     /locus_tag="VHS97_05525"
     CDS             3328..>3558
                     /locus_tag="VHS97_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447690.1"
                     /translation="MPIVTLQEAQANLAELVHHLAPGEGVTIMENDRPVAWLVVPLIA
                     PAIPPRPRPPVTGVPKAGQYEGRLVVPDDFKEP"
BASE COUNT          732 a         1075 c         1077 g          674 t
ORIGIN      
        1 gaggcgtctt cgcgccgtcg gtccacccaa ttcgctcaag tttctcccgg agacggccga
       61 cggaatcagg gggcgagtca tctacactga cttagcgcgg tacgcttgga atcggtcgcc
      121 cctggaagac agagaccgtg gatgccgtcc gcgctggttc gaggagttgc ttgtgcgttg
      181 ttgctggctt gggggactat tgcccaggcc caaaggacgg gaacgcgcgc gccagcggaa
      241 aacgacagcg cccttgggac gcagccgacg actgccagcc cagccgtcga gcaggaagat
      301 tcccctaagc ccgacgactc cacaccgagt tcccagccga aaactctgct tgagcgcttt
      361 cagaggttgc tctcgccaaa agaagatccc gatggcccga tcgacactga ccggcccacg
      421 ttcacgccgg cccacaccgt catacctccc gggcgagttc agatcgaata ggggtggacc
      481 tacaaccacc agaaaagtgc tgtgagaacc gacgacctct acgattttcc cgagctgtcg
      541 tttaggtacg gcttgatgga ccgggtcgaa ttgcgcacgc tctggatcgg gccgacattt
      601 gacgaagaat cggcggcaag ccggcgcgca cggcgcggaa ttggcggtgc ggccgacatg
      661 gaggtcggta tcaagtggca gttattcgcg ggtgacaaag agcggaagtg gattcctacg
      721 acggccttga tcacctcggc gattgcaccc actggaggaa gctcgcccct ctcatctggg
      781 gtcgccgagc cttacatcaa tctgatttac ggttggcacc cgaatgacaa gctcgcgctt
      841 tgcggcagta ccggctacct tggccagcga cagcaagccg tcgccggatc gggccttccc
      901 gccgacagct tccagcggtt tcaccagtca ctggttgctt tctactcagt tggggagcgc
      961 acgacggtct tttatgagtg gtacgtgctg atgttcacta acgcgcccaa caaccttccc
     1021 actcacttca tggacggcgg cctgctttat cgtctgacgc cgaacatcca ggtcgacctc
     1081 cgcgccggtt tcggcctgag tgggcgaccc gaagacgtct tcgccggcac gggattttcg
     1141 tttcgtttct gacgcgacgg aatctggata tccctcgccg ccacgatgtt gactgccata
     1201 atgcgtgctt catctcccca ggacgcgagc cgaatacctg ggcgaagacg cgctggcgtt
     1261 tttcgggctc tggtgttggg gagcaccgac aaaatggcga cgttggctca gcaggaaaag
     1321 ccacactgcc aggaccatga gcgccaggcc aatgggtaca attgacgtga agtcaggcag
     1381 gctagcagcc accacaatgg ccgttactgg cagtcgcacg agctcggaga tgagtgccca
     1441 ggggcgttgg tcgaagatgc cgccgacact gagcaaagtc cacaaaacta gcagggcggc
     1501 aatgatcagt tgggcgcggg agagcgactc gcccactgcc aagaggccga tcgcaagcag
     1561 tagggcggcc acgaattgca cgatcgcata agcatccaac ccgcgcggca cccgcgtatc
     1621 gtagaggacg acggtcgcgc gcgagacttc aggcgccgtc gggttagcgg gcaatccctc
     1681 gggtcgccat ccctgtggca tgaaccagat acgcagcttg tcaatccagc gcggcgcgag
     1741 ccaggcgtcg cagcacaatt cgctccagac gtggaggttg gcccacagtg ggttccaact
     1801 gttcaagggc ttggttaagc cgtacaccgg ctcttcttcc tcggcctgaa aggtgccgaa
     1861 catgcgatcc cagatgatca gagtgcccgc gtagttcttg tcgaggtact tggggttgcg
     1921 cgcgtggtgc acccgatggt gcgaaggggt gttcagcacc cactccagcg gtcccagctt
     1981 gccgatcgcc cgagtgttga tccagaattg atagagcaag tcgaagctta gcatcgccgc
     2041 aaaccatggc ggcggaaagc cgacgaaggc tagcggcaag tagaataccc acgaaaaaaa
     2101 gccctggaac gtcccctgcc ggagtgcgac cgccaggttg taatcttcac tcgaatggtg
     2161 cacgacgtgc cctgcccagg gggcgttgta ttcgtgggcg atgcgatgga accagtagta
     2221 agcgcaatcg acccccagga acaacacaat cgccgcgacc cacttgccac cggtcgagta
     2281 cgcctgaacg tcgatccaat tgaaacccgc gtgggttgca aaatcgtaag tcctcagata
     2341 tccggcaaac agcagaccct tgagaaaaat gccgacgacc tgctcggtaa tcccacagct
     2401 cagatcgttg atcgagtcgt ggaggcggta cgagtgctcg cgctgccaga gcgccacgac
     2461 cagttcgaca cctatcaata aaaagaagaa cggcactgcg taggtgatgt aggggacgtt
     2521 gcttcccagg gcgcggtcga tgaaggtcgg atccattcga tttccccatc agaaccgcga
     2581 accgtcggtc gggatctcgt cgacctcgca cgattgacga gtacggtgtc gtgcatcggc
     2641 accccgcaaa gcaattgatc tggctatcgt accgctgtcg tggccgaggc ggtaggcgca
     2701 gtgcgccgtt tcgccattgg ggtgcgccac cggcttctga cagctacacc ttgcgcggga
     2761 gcgatcgatc catcgcgggg cggcgaggct ccgtccgagc ttcttctgag tgctggctcg
     2821 gacggagcct cgccctcgcg agaaaacgtt gttcttcatc ccgcgaggaa aagcaaagac
     2881 ttgcgacgca cgccgccaac caccgaaccc ttcagccgga attccctcga cgcgacattc
     2941 gtgtggatgc tcgccgacca gaaggaacga caatgcgctg cggacgtaca gcggcccagg
     3001 gacgtgctac ggtcggagat tgaatattac gttttcgcgc tacgggaatt ggccgagcgc
     3061 aagcatcggc cgctggtccg aatcggctcc ggataaaccc gttagcgtcg tctgcctacc
     3121 gggacgaggg tggcggtgcg atgcgtgtcg tttccgcttt tacgctgttt ccgctcttat
     3181 ttaaaaggtc gcgcgaggat ccagaccatc gagccgactg tcttcgccaa cttgcgctac
     3241 tagccgctcg attccgcaat ggtcgagacg gtggccgact gtctggtaaa atgctcgtga
     3301 cgacttcgtt acttcggaga catcggcatg cccatcgtca ctcttcaaga agcgcaggcc
     3361 aacttggccg aactcgtgca ccatctcgcg cccggcgagg gtgtgaccat tatggaaaat
     3421 gaccggcccg ttgcttggct ggtcgttccg ctcattgcgc ctgcaattcc gccccggcct
     3481 cgaccgccgg tcactggggt gccgaaggcg ggccaatacg aggggcggct cgtcgtcccc
     3541 gacgatttta aggaaccg
//
LOCUS       DATAQL010000325        10816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1405728, whole genome shotgun sequence.
ACCESSION   DATAQL010000325 DATAQL010000000
VERSION     DATAQL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10816
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405728"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2507)
                     /locus_tag="VHS97_05530"
     CDS             complement(<1..2507)
                     /locus_tag="VHS97_05530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3447691.1"
                     /translation="MLDPQASRFWQSTLLSGLMDVQGLTACWEAIPPAKREVVEHIDR
                     RLARQAVQSKGLTLWQAQQLLAGRTSGFKVDRYILLDLIGQGGMGRVYLARDVRLNRR
                     VALKILSPERVNNPRAIARFQREARVGAQLQHENLVRIYDFGESNGRYYLVMEYIEGK
                     TVGSLISEQGQISPASTVRLVRQVALGLEHAHRKGLIHRDVNPYNILVTRDGTAKLAD
                     LGLAIDLADEDHVTREGATVGTFDYVAPEQARHSHSADIRSDIYSLGCTIYHMCAGRV
                     PFPSPSLPEKLFSHQALEPEPLEKLVPGIPVGLAEIVHRMMRKSPDERYATPIQVVQA
                     LEPYVEELANTGNNQEKSPLEAKDGPNYSSGLSPNIDSKAALAHQHAGEPVPVRIKTT
                     AVSHGLRVDPIAPTDASSSANSVEPIASLMSPISNAGTSDPEFPLLVNLGPEPSLSEG
                     LTRPKLKSTMDHKVSTGLVAKLHGRLAPLWLWMPVMLTATILLVVGLLTLVTRFATPP
                     WRTTLLVGLNKDHNSKSPADADIGKALSKTELPIVVRNESENEQQFPAERLIEALQTA
                     NGIRGWVELRNRRPIHLLGDQILDFTSGSGPLKVRAAPGTQPVIEIELKDAQSWLTTG
                     SAVALELSGLTIIVDYRQTTAGSAPPAVITAAGLVTFERCAFKVAGASSRQGSRAVFS
                     NGGRLNINRCWFEGFDNPVEIAAMINEPARIAQTMIVPTFDLAHPHRQPPEGYGWGVK
                     LQLDAGGVATTDNLKPHLILDDCTFVGAGLIDVTKSPVAFPLQIEVNHCVVVAKAVLA
                     CKPVMSLSSQVHWRGEGNQYDILGRFWIVLSASEGTPP"
     gene            2714..2893
                     /locus_tag="VHS97_05535"
     CDS             2714..2893
                     /locus_tag="VHS97_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HEX3447692.1"
                     /translation="MHSFQLGKRGATNTADLKSFNLAGIHWVIAGSESEGGPGARPLE
                     KDWVISIRYPLRWRP"
     gene            complement(2890..4572)
                     /locus_tag="VHS97_05540"
     CDS             complement(2890..4572)
                     /locus_tag="VHS97_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX3447693.1"
                     /translation="MVKIRRAYEVLFLGLFLFFLFITDLRYLKGWPVSLFLEATPLVA
                     VATALTTHTIYRNLVWGLVIIAVTMMLGRVWCNWMCPFGILHHFFGWIGNRRNTKQMI
                     EVNRYRKIYSIKYYILAVMIAMASLWIIPTAIDSPGQIWQAYQGAELQQKGVSRVVAA
                     IETGIARSAEEHKRSNSSLQIGLLDPIALTVRSMTTSVLPTVNKATESVYTEPREYWQ
                     AWVVGLIFLGFLAANWWIPRFFCRVVCPLGALLGIFSRFALWRIDRDPVRCTDCDLCL
                     KSCEGASDPQGDLRKSECFVCLNCIEDCPHDALSFRFLPRKASEVAYPAVGRRELVLS
                     GLFGLFFFPMARLSGGVRKNFQRSVIRPPGSVGEDEFLRRCIKCDQCIRVCPTNVLQP
                     ALFEAGLEGLWTPIMISKMGWCELNCTLCSQVCPTGAIREISIVEKLGIGPFEAKGPI
                     KTGTAFYNQGRCLPWAMDTSCVVCEEVCPVSPKAIFTRNVEVTDRWGATMELKRPYID
                     PVKCIGCGICEHECPVKDDPAVYVTAIGETRSKDRSLLLSLVDGQAQDWVSV"
     gene            complement(4744..5844)
                     /locus_tag="VHS97_05545"
     CDS             complement(4744..5844)
                     /locus_tag="VHS97_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX3447694.1"
                     /translation="MGEKLTRTQLKNLERFGGVNPADQHFSRRQFAAQVGGYALAAGG
                     AVAGGMLLQDRWGMEGVKPPPPVRLKNYSVSVPVGKPTLVVVRSRPVRPEDHASKDEE
                     LKAREEQAFDMVKAALNSMGGVDHFIQKGDVVVIKPNVAFDKNPDLAATTQPDTVSAV
                     TRLCFGAGARKVIVADNPINNPESCFYKTKVGEAAIRAGAELMLPKDSFFETLYVGGE
                     TITSTWRMFYRPFREATKVIGISPVKDHNLCKATVTMKNWYGLLGNPRNQFHQNIHGI
                     ISDFALMMKPSLVIADGRKLLMRNGPTGGSLNDVKRGDTMVIGTDHTAVDSWCVTKLL
                     ERRRDQIVYLDKVITRGLGQDWRPQWTQEITV"
     gene            complement(5938..7731)
                     /locus_tag="VHS97_05550"
     CDS             complement(5938..7731)
                     /locus_tag="VHS97_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEX3447695.1"
                     /translation="MISTGLWTQFRDELASDPLLVLALAAALTALATTPVAFAILGRL
                     DWFKARRGRILQRPEFASIVVGMLLVMGIPAIFAALVLKSRTFDRNRYEFDPNRTWSV
                     LEQGRGLQDLKEADAAVKLEMGRLAFERKNLVENVKKLDEAMLALRSVAGTSPAVAQR
                     IPSVLQSLAGVRKSVGVDGPQQLQDFTAPPVDLRAMSASGLANAPSMMVVPNASTGLV
                     PSTSPAPIAAAASGNGLSRAEIETELGTVPEPQRKIAAMLPLADLPAEWTVGKLAERH
                     LETFNADNLYEKIDGRAESFIQYGVKGMAYAFYHPTGDPSNELQLYIFEMADALKALG
                     KYGSEKPDEFKAVPIGNEGYTTAGSTLFYSGRYYTQIVSTQDDPKFGAFALELAKRVA
                     ASQAPGGSKAPVGTGAAGTSSPAAAASGPSTGTPSAVASAEKAVGSEGETKARPAPTK
                     PAQPDFTPADYFALLPAAGRQGDAKYVAQDVFGYSFLSDVFMADYKSGEVSWQGFIRP
                     YRDANEAKDVLAKYIISVKKDGAEVKTVVADGADELVISNNIGLVDIIFRKGNTLAGA
                     NGATSAPPAEEFARALAKSLPATVHTLDSAN"
     gene            complement(7941..8807)
                     /gene="ftsY"
                     /locus_tag="VHS97_05555"
     CDS             complement(7941..8807)
                     /gene="ftsY"
                     /locus_tag="VHS97_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX3447696.1"
                     /translation="MAKTAQLFNFRSWFGRKVDQSFLDDLESRLIQADVGVVATTRII
                     DRVREAYSEKTADENLIKFVKAELKGLLEDLRPGALRVAPKPPTVYLIAGVNGSGKTT
                     SIAKLAQYLKDQGNSILLAACDTFRAAAADQLTVWAGRAGCEIVKGAPGADPASVAHD
                     ACQRALARHIDVLIVDTAGRLHTQTHLMRELEKIRNVIQRLIPGAPHEVLLVIDATNG
                     QNAIRQAEVFTKSIGCTGVILTKLDGTAKGGVVVAVRQSMNLPVKFVGIGEALDDLQP
                     FDADIFVESLFL"
     gene            complement(8934..9872)
                     /locus_tag="VHS97_05560"
     CDS             complement(8934..9872)
                     /locus_tag="VHS97_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3447697.1"
                     /translation="MKSTLRKTAATIRSSPPGYPRDGADLHVHTTHSDGACSPCEVVV
                     AAARVGLKALAITDHDTVSALAVARPEAARWGIELVAGVELTCEYDGREYHILGHFIR
                     DDDPALLKGMASLRAGRAQRIEAMAARLHSLGLSIDLDAICRVFPRASLGRRHLADYL
                     ARTRQVGSTREAFARFLGDGCPACVDKPRLDSGLAIALIKGAGGVAALAHPPHDLREA
                     ILRRIADQGLHAIEVDGPGFSNGKSRRLRFWADQLGLVGIAGSDFHAADRPGRWVGAI
                     TTPPKDLERLRTLSRTCLVDHVPLNPPTVGSIKSVR"
     gene            complement(9853..10356)
                     /gene="nusB"
                     /locus_tag="VHS97_05565"
     CDS             complement(9853..10356)
                     /gene="nusB"
                     /locus_tag="VHS97_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX3447698.1"
                     /translation="MTRRSRGREVALQVLYQVEQNPAIPADEIRRFIERRLLEDHKLC
                     EFTHGLIDGVKEHQARIDAMISQVAENWRLDRMAAIDRNILRLGAYEMLFRPEVPAKV
                     AINEALELAKRYSTAQSSRFVNGILDRVLQYQIQEASPQPDPEAASHTEPETSPLEQP
                     PDEINPA"
     gene            complement(10604..>10816)
                     /locus_tag="VHS97_05570"
     CDS             complement(10604..>10816)
                     /locus_tag="VHS97_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447699.1"
                     /translation="LRSLSITEILSVPVPTRPAMAVDRCATHYYDAPVHFSRPDQSLV
                     TRCGGAIATPALSHSAIFIGNLSPAR"
BASE COUNT         2192 a         3115 c         3208 g         2301 t
ORIGIN      
        1 gggggtgttc cctcgctggc cgaaaggacg atccagaaac gcccgaggat gtcgtattga
       61 ttaccttcac cccgccaatg gacctgagag gaaagcgaca tgacaggttt gcaggccaag
      121 acagccttcg ccactactac gcaatgattc acctcgatct ggagcggaaa tgctaccggg
      181 ctttttgtca cgtcaatcag acccgccccc acaaatgtgc agtcatcgag gatcaagtgg
      241 ggtttgagat tgtctgtcgt cgctacacct ccagcatcaa gctgaagctt caccccccag
      301 ccatagccct ccggtggctg tctgtgtggg tgagccagat cgaaggtagg aacgatcatc
      361 gtttgcgcaa ttcgcgcggg ttcgttgatc atggcagcaa tttctacagg gttgtcgaac
      421 ccttcaaacc agcaacgatt gatatttagt ctgccgccgt tggagaagac tgcgcgggac
      481 ccttgtcggc tggaagcacc ggcgactttg aatgcacatc gttcgaacgt gaccaagcca
      541 gcggctgtta tcaccgccgg tggggctgag cccgccgttg tttggcgata gtcgacgata
      601 atcgtcaatc cggatagctc gagtgccacg gccgagccgg tcgtcaacca cgactgggcg
      661 tctttcagct cgatctcgat aacgggctgg gttccgggag cggcccgcac tttcagtggt
      721 ccgctcccgg acgtgaagtc gaggatctga tcaccaagca aatgaatcgg tcggcggttc
      781 cgtagctcaa cccacccgcg gatgccgttg gcggtctgaa gtgcctcgat cagcctctca
      841 gcggggaatt gttgctcatt ctcactctcg tttcggacga caatgggtag ctcggttttc
      901 gatagcgctt tcccgatatc agcatcagcc ggcgatttgg aattgtgatc tttgtttaag
      961 ccgacaagga gggtagtgcg ccatggagga gtcgcaaacc tagtgaccaa tgtcaggagt
     1021 ccgacaacga gaaggatggt cgctgttagc atgaccggca tccagagcca gagcggcgcc
     1081 agccgcccgt gcagcttcgc gacaaggcca gtggatacct tgtggtccat cgtagatttt
     1141 agcttgggtc gagtcagtcc ttcgctaagt gacggttctg gaccaaggtt gacgagcaat
     1201 ggaaattcag ggtcgctcgt tccagcgttg ctgatggggc tcatcagaga agcaatgggt
     1261 tccacagaat tagccgatga actggcatcg gtaggagcaa tcgggtcaac tctcaaacca
     1321 tgcgatacgg ccgtcgtttt gatacgaact gggacgggct cgcccgcgtg ctgatgcgcc
     1381 agggcggctt tggagtctat gttcggagat aagcccgagg agtaattagg tccatccttg
     1441 gcctccagcg gggatttttc ttggttgttc cccgtatttg caagttcctc gacgtaaggc
     1501 tcgagtgcct gaacaacctg gatcggtgtg gcatatcgct cgtcgggtga cttgcgcatc
     1561 atccggtgga caatctcggc aaggcctact ggtattcctg ggactaactt ctctagcggc
     1621 tcgggctcta gcgcctggtg cgagaagagc ttttcgggca ggctcgggct ggggaatgga
     1681 acccggccgg cacacatgtg ataaattgtg cagcctaatg agtaaatatc actgcgaatg
     1741 tcggcggagt gcgaatgccg cgcctgttct ggcgcgacgt aatcaaatgt cccgaccgtg
     1801 gctccctctc gggtaacatg gtcttcgtcg gctaggtcaa ttgccaaccc gagatcggcc
     1861 agcttggccg ttccatcgcg ggtgaccaga atgttgtatg gattgacatc gcggtggatc
     1921 agacctttgc ggtgagcgtg ttccagtccc agcgcgacct gtcggactaa gcgcacggtg
     1981 ctggccggcg aaatctgacc ttgctcggag atcaagcttc ccacggtttt tccctcgatg
     2041 tattccatta cgaggtagta gcgaccgttg gattccccga agtcgtaaat tcggaccagg
     2101 ttttcatgct gtaactgagc gcctactcgg gcctctcgct ggaatcgcgc aattgctcgt
     2161 gggttattga cccgctctgg ggacagaatc ttgagcgcca ctcggcggtt aagccggaca
     2221 tcgcgcgcga ggtagacccg tcccattcct ccctgaccga tcaagtccag aagtatgtag
     2281 cgatcaactt tgaacccgct ggtgcgaccg gccaagagct gctgtgcctg ccacagagtc
     2341 agccctttcg attgaacggc ctgtcgcgcc aatcgtcgat caatatgctc tacaacctct
     2401 cgctttgccg gtggaatcgc ttcccagcag gcggtcaacc cttgaacatc catcagcccg
     2461 ctcagaaggg tcgattgcca gaatcgcgac gcttgaggat caagcatggg gcatcctgaa
     2521 actgctgcgc tttcatgccc agcgtattac cacgacgacg tggtcaacta ccagatacca
     2581 tagcatcttc gtctatcaaa gttaacgaag caaagccccc gtggctctta gtcgcacgcg
     2641 cgatagagtt taagtcagca tatcagctac catgccttga agccatcccg gcttggaact
     2701 ctgccccttg cgggtgcatt cgtttcaact tggcaagcgc ggtgctacca atacagcgga
     2761 tctcaagtcg ttcaacttgg ccggaatcca ctgggtcatt gcgggaagtg agagtgaggg
     2821 tggtcctgga gctcgaccgt tggaaaagga ttgggtgatc tcaatccgtt atccattgag
     2881 gtggcgacct tagactgaca cccaatcctg cgcttggcca tcgaccaacg agagcaggag
     2941 cgaacggtct ttgctgcggg tttcaccgat tgcagtcaca taaacggccg ggtcgtcctt
     3001 caccgggcac tcgtgctcgc agatgccgca tccgatacac ttgaccgggt cgatgtacgg
     3061 acgtttgagt tccatggttg ccccccagcg gtccgtcacc tccacgttac gagtgaatat
     3121 cgccttgggg ctcaccgggc agacttcttc gcaaacgacg caactggtat ccattgccca
     3181 aggaaggcag cggccttggt tgtagaaggc tgtaccggtc ttgatcggcc ctttggcttc
     3241 gaagggtccg atgccgagtt tttcgacgat agagatctct cggatagctc cggtcgggca
     3301 gacctggctg cagagcgtgc agttcagctc acaccatccc atcttcgaga tcatgatcgg
     3361 cgtccagagc ccctcaagtc cggcctcgaa cagagccggt tgcaacacgt tggtcggaca
     3421 gacccgaatg cactggtcgc acttgatgca tcgccggaga aactcatcct ccccgaccga
     3481 gcccggggga cggatcacgc ttcgctggaa gttcttacgc acgccccctg agagccgggc
     3541 catcgggaag aagaacagac caaagagtcc agagagaacc agctcgcggc ggccgaccgc
     3601 cggataggct acctcgctgg ccttgcgggg cagaaatcgg aacgacaggg cgtcgtgggg
     3661 acaatcttct atacagttga gacagacgaa gcactcgctc ttgcgcaggt cgccctgcgg
     3721 gtcagacgcg ccctcacagc tcttcaagca taggtcacag tcggtgcagc gaaccgggtc
     3781 gcgatcgatt cgccaaagtg caaaacggct gaaaatccct agcagcgccc ccaaaggaca
     3841 gacgacgcgg cagaaaaacc ggggaatcca ccaatttgcc gccaagaagc caaggaagat
     3901 caaccctacc acccaggctt gccagtattc gcgcggctcg gtgtagacgc tttctgtcgc
     3961 cttgttgacg gtgggcaaaa cgctggtcgt catcgagcgg acggtgagag caatcgggtc
     4021 caaaaggccg atttgcaacg agctattgct tcgcttgtgc tcttcggcgg agcgagcgat
     4081 tccggtctcg atcgctgcta ccactcggga aacgcccttt tgttgcagct ccgccccttg
     4141 atatgcctgc catatctggc ccggcgaatc gatggccgtg ggaatgatcc agaggcttgc
     4201 catcgcgatc atcaccgcga ggatatagta cttgatcgaa tatatcttgc gatatcgatt
     4261 aacctcgatc atctgcttgg tgttgcgccg gttgccgatc catccaaaga aatggtggag
     4321 aatgccgaac gggcacatcc aattgcacca gacccggccg agcatcatgg tgacggcgat
     4381 gatcaccaag ccccagacta aattgcggta gatcgtatgc gttgtgagtg cggtggcgac
     4441 agcgacaagc ggcgtggctt ccagaaagag cgacaccggc caccctttga ggtagcgcaa
     4501 gtcggtgatg aagaggaaga aaaggaaaag ccccaagaag aggacttcat acgcgcggcg
     4561 gatcttgacc atcggtccct ccggcaggga gattgagcta ttttggatgc ctaggggcaa
     4621 tcttgcttcc gcgggcgcgg atcgcgttca acaggcactc gggtatgcgt tacaacgaat
     4681 ccaaagacga ttcgtctgca gcgctgcttc cgtatcgggt actttaacca tcgatcgtcg
     4741 gtatcagacc gtgatctcct gagtccactg aggccgccaa tcctggccta gtccgcgggt
     4801 gatgactttg tcgaggtaga cgatctgatc gcgacggcgc tccagcagct tggtaacgca
     4861 ccaactatca acggccgtgt ggtcggtgcc gatcaccatc gtgtcacccc gcttgacgtc
     4921 gttcaagcta cccccagtcg ggccattgcg cattaacaac ttacgcccgt cggcgatgac
     4981 cagcgagggc ttcatcatca aggcaaagtc agagatgatc ccgtggatat tctggtgaaa
     5041 ctggttgcgc ggattgccga gcaggccgta ccagtttttc atggtcaccg tggccttaca
     5101 gaggttgtgg tccttgaccg gtgaaatccc gatgactttg gtcgcctcgc ggaacggccg
     5161 gtagaacatc cgccaagtgc tggtaattgt ctcgccgcca acgtaaagcg tctcgaaaaa
     5221 gctgtctttg ggcagcatca actcagcacc ggcgcgaatg gcagcctcac caactttcgt
     5281 cttatagaag cagctttcag ggttgttgat cgggttatcc gcgacgatga ccttgcgtgc
     5341 gccggcccca aagcaaaggc gcgtaacagc cgagacggtg tcaggctggg tggttgcagc
     5401 aaggtcggga ttcttgtcaa aggcgacgtt gggcttgatc accacaacat cgcccttctg
     5461 gatgaagtga tccactcccc ccatggagtt gagcgcggcc ttgaccatgt caaaggcttg
     5521 ctcttcgcgc gccttgagct cttcatcctt ggaggcgtgg tcctcggggc gaacgggtcg
     5581 cgaacgaacc acgactagtg tgggctttcc aaccggcacc gacaccgagt agttcttgag
     5641 tcgaaccgga ggcggtggct taaccccttc catgccccag cgatcttgaa gaagcatacc
     5701 tccagcgacg gctccgccgg ccgccagagc gtacccaccg acttgggcgg cgaattgccg
     5761 gcgggaaaaa tgctggtccg ccgggttcac cccgccaaaa cgttccaggt ttttcagttg
     5821 ggtccgggtc agcttttcgc ccatcgtatt cctcggttgg ggtagtaacg tttcaacgcg
     5881 aagtccgcga agtaaaaacc gagaggtgca ggaagcgact gtttttcatg ggttcagcta
     5941 gttagcgcta tcaagggtat gtaccgtggc cggcaggctc ttggccaatg cgcgggcaaa
     6001 ctcttcggcg ggcggtgcgc tcgtggcgcc attcgctccg gccagggtgt tacccttgcg
     6061 gaaaatgatg tcgaccaggc cgatgttgtt gctaatgacc aattcatcgg ctccgtcggc
     6121 tacgaccgtc ttgacctcgg cgccgtcttt tttgacgctg ataatgtatt tcgcgaggac
     6181 atccttggcc tcattggcgt cgcggtaggg tcgaatgaat ccctgccaag aaacctcgcc
     6241 actcttataa tcggccataa agacgtcaga gagaaaactg tagccaaaga catcctgcgc
     6301 gacgtacttc gcgtctcctt gccggccggc ggctggcaag agagcgaaat aatcggccgg
     6361 agtgaagtcg ggctgggctg gtttggttgg ggctggccgt gccttggttt cgccctctga
     6421 acctacagcc ttttctgcgg acgctactgc cgaaggcgtt ccggtggagg gtccgcttgc
     6481 ggccgcagcc gggctggatg ttccggcggc gcctgtgcct acgggtgctt tggaaccccc
     6541 cggtgcctgg ctggccgcca cccgtttggc cagttccagc gcgaaagcgc cgaacttggg
     6601 gtcatcctgg gtcgagacga tctgggtgta gtaccgcccc gagtaaaaca atgtactgcc
     6661 ggctgtggtg taaccctcgt tgccgatcgg gacagccttg aactcgtcgg gtttttccga
     6721 gccatacttg cccaacgcct tcaaagcatc tgccatctcg aagatgtaaa gttgcagctc
     6781 gtttgatggg tctccggtcg ggtggtaaaa ggcgtaagcc attcccttga cgccgtactg
     6841 aatgaagctc tcggcacggc cgtcgatttt ctcgtacagg ttgtccgcgt tgaaggtttc
     6901 caggtggcgt tcagccagtt tgcctacggt ccactcggcc ggcaagtcgg cgaggggcag
     6961 catggcggcg atcttgcgct ggggctcagg cacggtcccc aactcagtct cgatttcagc
     7021 ccggctcaat ccattaccgc tcgccgcagc agcaattgga gcaggggatg tacttgggac
     7081 cagcccggtc gaagcattgg gcacaaccat catgctcggg gcattcgcca atccactagc
     7141 actcatcgct cggaggtcga ccggcggcgc ggtgaaatcc tggagctgct gggggccatc
     7201 cacgccgacg ctcttgcgca cgccggccag cgactgcagc acgctgggaa tgcgttgcgc
     7261 tacggccggc gacgtccccg caacgcttcg cagcgccagc atggcctcat ccagcttttt
     7321 gacgttttcg accaggtttt tgcgctcgaa tgcgagccgc cccatctcca gctttaccgc
     7381 ggcgtcggct tccttgaggt cctgtaaccc tctaccttgt tccagcacgg accacgtgcg
     7441 gttggggtca aactcgtaac gattgcggtc gaacgtgcgg ctcttgagca cgagcgctgc
     7501 gaagatggct gggattccca ttacaagcag catgcccaca acgatcgatg caaattctgg
     7561 gcgttggaga atccgccctc gccttgcctt gaaccagtcg aggcgcccta gaatcgcgaa
     7621 cgccacgggc gtggtcgcca gtgcggtcag ggcggccgca agcgccagaa ccaacaacgg
     7681 gtcgctcgcc agctcgtcac gaaattgcgt ccaaaggcct gtcgaaatca tcgtgggccc
     7741 actgtaaatc ttgaatggac gagtgtgacg gccacggtgc ctagctcgcc atgtgtcttg
     7801 ccagataagg gagggagagg agtcgactcg cgaccacctc gagcgattat actggcgtcg
     7861 gggggcgtgc ggcaacgatt tcagtcggag gattccgtat tgagccagtt tttagccaaa
     7921 agttcataca aagaatcggt ctatagaaac agcgactcga caaagatatc ggcgtcaaac
     7981 ggctggagat cgtccaacgc ttcgccaatc cctacgaatt tgacaggtag attcatggat
     8041 tgacggacag ccacaacgac gcctccctta gccgtgccat cgagcttggt tagaatcacg
     8101 cccgtacagc cgatgctttt ggtgaagacc tcagcttgcc gaatcgcatt ttggccgttg
     8161 gtggcgtcga taaccaacaa cacctcgtgc ggtgccccgg ggattagccg ctggatgacg
     8221 ttacggatct tctcgagctc gcgcatcaag tgggtctggg tatggagccg gccggccgta
     8281 tcaacgatca agacgtcgat gtgccgggcc agcgcccgct ggcaagcatc atgggctacg
     8341 ctggccggat cggccccagg cgctcccttg acgatctcac agccggcccg accggcccat
     8401 accgtgagtt ggtcggcagc ggctgcgcga aatgtgtcgc aagcggctag caggatcgaa
     8461 ttcccttggt ctttaagata ctgtgcgagc ttggcaatcg aggttgtctt gcccgagccg
     8521 ttgactcccg cgatcaagta cacggtcggt ggcttgggcg cgactcgaag cgctcctggc
     8581 ctaaggtcct caagcagccc cttgagctcg gccttgacga acttgatcag gttttcgtca
     8641 gcggtctttt ccgagtaggc ctcacgcacc cgatcgatga tgcgagtcgt cgcgactacc
     8701 cccacgtctg cctgaatcag acggctttcc aggtcgtcga ggaacgactg gtccaccttg
     8761 cggccgaacc acgagcgaaa gttgaaaagc tgggcggttt tggccaagcc tttcttaaat
     8821 cgttctatca atccagtgat tgccatttgc gtctttcgac ccgctgcatc aaatgaatcc
     8881 gcgggttccg ggatcgctaa gttcgagacc cagaccccca tttcgtgccg aggctatctt
     8941 accgatttga tcgaaccgac cgtaggcgga ttcagcggca catgatcgac caagcacgtc
     9001 cggctgagtg tccgtagccg ttccagatct ttggggggcg tggtgatggc gccgacccag
     9061 cggcctgggc gatcggccgc atggaagtcg ctgccggcaa tgccgacaag tccaagctga
     9121 tcagcccaga atcgaagtcg ccggctcttg ccattggaaa agcccggtcc atccacctcg
     9181 atcgcgtgca atccttgatc ggcgattcgt cggagtatgg cttctcggag atcatgggga
     9241 ggatgggcca gcgcggcgac tccaccagct cccttgatca aagcgattgc cagaccgcta
     9301 tccagccgcg gcttgtccac gcacgccggg caaccgtccc cgaggaaacg agcgaaggcc
     9361 tcgcgcgtac tgcctacttg tcgagtccgt gcaaggtaat cggcaaggtg gcgccgaccc
     9421 aagctggcgc gggggaagac gcgacaaatt gcgtcaagat cgatcgaaag ccccaggctg
     9481 tgcagtcgtg cagccatggc ctcgatccgt tgggctcggc cagcgcgaag tgatgccatt
     9541 cctttgagaa gagccgggtc gtcatcgcga atgaaatgcc cgaggatgtg atattcacgt
     9601 ccatcgtact cgcacgtgag ctcaactccg gcaaccagct cgatacccca ccgggctgcc
     9661 tcgggccgcg ccaccgcgag tgctgagacc gtgtcatggt cggtaattgc cagggctttc
     9721 aagccaaccc gtgccgcggc gacgacgact tcgcatggag agcacgctcc gtcggagtgc
     9781 gtcgtatgta catgcaggtc ggcaccatca cgcgggtatc ctgggggcga cgaccgaatc
     9841 gttgcagcgg tcttacgcag ggttgatttc atccggcggc tgctcgagag gagatgtttc
     9901 cggctcggta tgactggccg cctccggatc tggttgtggg ctggcctcct ggatttggta
     9961 ttgaagcact cgatcgagga tgccgttaac gaatcggctc gactgcgcgg tgctgtaccg
    10021 tttggccagc tcgagagcct cgttgatggc gacctttgcg ggcacctcgg gccgaaacag
    10081 catttcatag gctccgaggc ggaggatgtt gcggtcgatt gccgccatcc gatcgagccg
    10141 ccagttttcg gcgacctggg agatcatcgc gtcaatccgc gcttgatgtt ctttgacgcc
    10201 gtcgatcaac ccatgagtaa actcgcagag tttgtggtct tccaggagcc tccgctcgat
    10261 gaaacggcga atctcatcgg caggtatcgc gggattctgc tcgacctggt acaggacctg
    10321 gagagcgact tcgcggcctc gggaacgtcg cgtcatgatc aggtggcctc accctggatt
    10381 cggttcactt cgtccaagcc gatcatacgc caaggcgggc taagccgaaa agggggctag
    10441 agcggtgggc cgcgtggatc gccaagctct ggtcgcggct gggcgcacga aagaccccgt
    10501 acgcggtctt gatgggtgca tttttggata aacgccgtac ggcgaactct ggtgcaaatc
    10561 tttgcaccgt cgagctcatt aattccggtt aaacgcattt gactcatcga gcaggggaca
    10621 agttgccgat gaaaatagca gagtgggaaa gcgcgggcgt cgcgattgcg ccgccacagc
    10681 gggtcaccaa cgactggtcc ggcctgctaa agtgtaccgg cgcatcgtag taatgggtcg
    10741 cgcatcgatc aacggccatt gccggtcgtg tcggaaccgg cacggaaagg atttcggtta
    10801 tgctcagact ccgtaa
//
LOCUS       DATAQL010000326         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2627622, whole genome shotgun sequence.
ACCESSION   DATAQL010000326 DATAQL010000000
VERSION     DATAQL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627622"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="VHS97_05575"
     CDS             <1..1863
                     /locus_tag="VHS97_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3447700.1"
                     /translation="VKDFAIFTVDPQGLVVTWNPGAERLFGYPEKEILGQQLAILFTP
                     EDRATGIPDQEIATAAAKGRSSDERWHQRKDGGRFFASGVLTPIFDEENRLRGFAKIA
                     RDITERKQADEAVREAAVRLKAIVETAVDGIITIDERGLIESINPAAERIFGYSHQEV
                     VGRNITMLMCDPERGQHDHYLNEYLRTGRQKIIGTIREVRGLRKDDSVFPMELAVSET
                     RLGVRRIFTGIVRDVTEFKRAVEALKDADQRKDQFLAMLAHELRNPLAPISNAVQIMQ
                     LEGPNGPSFQWSTEVIEDQIKHMTRMVDDLLDVSRITRGKVDLQKETVKLADVVELAF
                     EASRPTIDAYHHQLNVILPDEPIVLEVDPPRLAQVLSNLLNNAAKYTDEGGHISLTAE
                     KVGQEVLIRVRDSGIGIAPDLLPKVFELFTQADQTLSRSRGGLGIGLTLVRSLVELHD
                     GRVTAHSDGPGQGSEFLVRLPLAAKSESGTAADVEAVSPTNEGLPCRRILVVDDNRSN
                     AQSLELLLRALGQEVHTAFDGPAALELARKHRPEVILLDIGLPIMDGYEVARRCREDP
                     GFLDLTLVAMTGYGQDSDRQRSQEAGFDAHLVKPVYLDDLRLLLKRPGLLSSKS"
BASE COUNT          519 a          749 c          673 g          438 t
ORIGIN      
        1 gtcaaggatt tcgccatttt cacggtcgat ccccagggac tggttgtcac ctggaacccg
       61 ggcgccgagc gtctgttcgg ctatcccgaa aaagaaatcc tgggtcaaca gctcgcaatt
      121 ctcttcactc cggaagaccg agccaccggc attcctgacc aggaaattgc gaccgcggcg
      181 gccaaaggcc gctcgtccga cgagcgatgg catcagcgca aggacggtgg ccgtttcttc
      241 gccagcggcg tgctaactcc catctttgac gaggagaaca ggctccgggg tttcgccaag
      301 atcgcacgcg atatcaccga gcgcaaacaa gccgacgagg ccgtccgcga ggccgccgtt
      361 cggctcaagg cgatcgtcga gaccgccgtc gatggcatca tcactatcga cgagcggggg
      421 ttgatcgagt caatcaaccc cgccgccgag cgcatcttcg gttattctca ccaagaagtc
      481 gtcggccgca acatcacgat gctcatgtgc gatcccgagc gcggccagca cgaccattac
      541 ttgaacgaat atctccggac cggacgacaa aagatcatcg gcacgatccg cgaagtgcgc
      601 gggctccgca aggatgattc cgttttccct atggagctgg ctgtcagcga gacgcgactg
      661 ggcgtgcgcc ggatttttac cgggatcgtc cgcgacgtca ctgaattcaa gagagccgtc
      721 gaggccctca aagacgccga ccagcgcaag gaccagtttc tcgccatgct cgcccatgag
      781 ctccgcaatc cgttggcccc gatcagcaat gccgtccaaa tcatgcaact ggaaggaccc
      841 aacggaccga gctttcagtg gtccacagag gtgatcgagg accagattaa acacatgacg
      901 cgcatggtcg acgatcttct cgacgtttcg cggatcacgc gcggcaaggt cgaccttcag
      961 aaggaaactg tcaaattggc ggacgttgtc gagctcgcat ttgaagccag caggccgacg
     1021 atcgacgcgt atcatcatca gctcaatgtc atcctcccgg acgaaccgat tgtcctggaa
     1081 gtcgacccac cccgtctcgc gcaggttctg tccaatctcc ttaacaatgc cgccaaatac
     1141 acagatgagg gaggacacat ttccttgacc gccgaaaaag tgggtcagga ggtcctgatt
     1201 cgcgtccgcg atagtggaat cggcatcgct cccgacctgc tgccaaaggt cttcgagctt
     1261 ttcacccagg ccgatcaaac actgtctcga tcgcgggggg gattgggaat cggattaact
     1321 cttgtgcgct cgctggtcga attgcacgac ggccgcgtaa ccgcccacag cgacgggccg
     1381 ggccaaggaa gcgaattcct ggttcggctc ccgctggcag ccaagtcaga atcgggaacg
     1441 gcagccgatg tagaggccgt ttcaccgaca aacgaagggc taccgtgccg ccgaatcttg
     1501 gtcgtcgacg acaatcgcag taacgcccaa agcctggagc ttctgctccg agcgcttggc
     1561 caagaggtcc acacggcatt cgacggcccc gcggcgcttg aactggcgcg aaaacatcgg
     1621 ccggaagtca tcctccttga catcggtttg ccgataatgg atggctacga agtggcccgc
     1681 cgctgccgag aggatcctgg ctttctagac ctgacgttag tggccatgac gggctacgga
     1741 caagactcag atcgtcagcg ctcgcaggag gcgggcttcg atgctcacct cgtcaagccc
     1801 gtctatctcg atgatctccg gctgctactc aagcgaccag gcctcctgtc ttcaaagtca
     1861 tagcttccgc tatgctggcg actcgaggga ttgccggaga gccggtcatg tccggtcaag
     1921 tgctgaagat aacacgccca cgaacacaga gaggaaccgc aaagcgggca aaatacagcg
     1981 tttcgaagcc gtccgccgca tacaagacaa cggctcgagt tgttcatgtg ctgccctgcc
     2041 acgattgctt cctctgcgcc ccaagcggct gagccgtcac tcgccggctc tgagaccaaa
     2101 agcttgcggc cctttcactc gaccctgctc acgaggcgac gcccatagcc ttgaccatgt
     2161 tgcgacattc gagagaaact cgattcaccg gggcccgccc cacgattggg gcgaccgtca
     2221 cttcattcat ttgcctcgca agtgaaagac cggcacgagc cgcccctcgt tcggggcaac
     2281 ccgcaccgga aactaggggt cgtctattcg agtcggctta gcgggagcgc agtgctaggc
     2341 cgaccacgac ccctagtagt acacctgcgc caaaggccg
//
LOCUS       DATAQL010000327         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1100605, whole genome shotgun sequence.
ACCESSION   DATAQL010000327 DATAQL010000000
VERSION     DATAQL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100605"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..2547
                     /gene="nuoL"
                     /locus_tag="VHS97_05580"
     CDS             181..2547
                     /gene="nuoL"
                     /locus_tag="VHS97_05580"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX3447701.1"
                     /translation="MSWQVGLYAVAVLIPLAAFTIEAIFIRQLKRFNAYLATGAIGFS
                     CLLALIGFVDYAIESGGFAHHAEAAAQGEQEPALSPDESAESGGHHQPLVWSGKADWL
                     VLGGRTATTADQTGKEVAKPDLVVSLGVAIDNLTVVMFLMVTFIATLIHIYSMGYMHD
                     DPRYPLFFTYLSLFCFSMLGLVVSANVFMIFIFWELVGVCSYLLIGFWYEEKVNSDAA
                     NKAFIVNRIGDVGMLVGLGILWTSLGTFDFQEINQGLRGPAGKLNLVRPSGEVLVELV
                     DPASHQVKTDEATGLPRRIPLGMLTIAGLGVFAGCVGKSAQFPLHVWLPDAMAGPTPV
                     SALIHAATMVAAGVYLVGRFFPLFTNDVLLYIAYTGGITLFIAATIAMVQTDYKKVLA
                     YSTVSQLGFMMLGLGVGGWAAGLFHLLTHAFFKALLFLGAGSVYHSVHTYEMPPLGGL
                     LKKMPITAWTMLVGTLAISGVPLFSGFYSKDAILAAALARVIGSPEHILLFLFPAVGA
                     ILTAFYMFRMWFLTFAGEPRGFTSKVEAVHSHVHDEEHALDKDHGVGHGHGHGHDLNP
                     AAHAHESEPIMTWPLIILAVCSVFAGWTLFYIIPLPFGTPTLEHILEYGEPYRATDIL
                     AMHHYYAFGASLVIMLTGIGLGLLYYAPHGFPYFVPTRFSAVRTAERFGGVYRFLVHK
                     WYFDELYWAVFVRPCLALARLCRQLDQVLVDGLVNASASATAFLSRRGGWFDLIGVDQ
                     IVNLTAKAVYVTGDWSRSIQTGKLRNYLMFLSVMLVVLFAGVLVWIRG"
     gene            2630..>3751
                     /locus_tag="VHS97_05585"
     CDS             2630..>3751
                     /locus_tag="VHS97_05585"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX3447702.1"
                     /translation="MSDNLLLTSLWLVPLIGLVTVLILPKPAEQAVKWVSLGFTSLTF
                     LITLVVLGIYLGEGLKDKSLRERVVNNKLVSSAGMELTESDETGGESDLVVRRAWIPY
                     FNIQYYLGVDGISLSLVVLTGLVSVLACLASWNIEKQVKGYFALYLLLCASMIGVFIS
                     LDLFLFYVFFEVMLLPMYFLIAIWGGPRREYAAIKFLLYTLFGSVFILVAILILYFWQ
                     SGVTVPGFSGHSFDIVQLTTVASETSYYPRSIQWWIFILFFTGFIVKLPSFPFHTWLP
                     DAHVEAPTPISMILAAILLKIGGYGLIRLAWPLAPAGAYDWSVWVGILGVFSIIYGAL
                     VAMAQTDFKKLVAYSSVSHMGYVTLGMAVMGIFAADGSRL"
BASE COUNT          633 a         1073 c         1120 g          925 t
ORIGIN      
        1 agcgtcgtgc gcggcgagtc gcggaatagg ggactcgtgt ttttcgatcg gttcgaccac
       61 gatcgtcttg cccggaaagg gcaacggcga actgcggaca tgccgccgtt cgatgggcaa
      121 gcccggtgga gtcgatgtct cctcaagggc tctcgggata ttaccaaggg acgatacgag
      181 atgagctggc aggtgggtct ctacgcggtt gcggtcttga tcccactggc cgccttcacg
      241 atcgaggcga ttttcatccg gcagttgaag cggttcaacg cgtacctggc caccggcgcg
      301 atcggctttt cctgtctgct tgcgttgatc ggtttcgtgg attatgcgat cgagagtggc
      361 ggattcgcgc atcatgccga agccgccgcc caaggagagc aagagcccgc cctatcgccc
      421 gacgaatcgg ccgaaagcgg cggtcaccac cagccgttgg tttggtcggg caaagcggat
      481 tggttagtcc tgggcggccg cacagctacg acagccgatc agactggtaa agaggtcgcc
      541 aagcccgacc tggtggtttc cctcggcgtg gcgatcgaca acttgactgt cgtgatgttc
      601 ctcatggtca cgttcatcgc gaccttgatc cacatctact cgatgggtta tatgcacgac
      661 gacccgcgct atccgctatt cttcacttat ctttcgctgt tttgcttttc aatgctgggc
      721 ttggtcgtct cggcgaatgt cttcatgata ttcatcttct gggagctggt gggcgtctgt
      781 tcgtacttgc tgatcggctt ctggtatgag gaaaaggtca acagcgacgc ggccaacaag
      841 gcgttcatcg tcaaccggat tggcgacgtc ggcatgctgg ttggcctggg catactctgg
      901 acgagcctgg gtacttttga ttttcaagag atcaaccagg ggttgcgcgg cccagcgggc
      961 aaattgaatc tcgtccggcc aagcggcgaa gtgctggttg agctcgttga tcccgcgagt
     1021 caccaggtca agaccgacga agccaccggg ctgccacgcc ggattccctt ggggatgctg
     1081 acgatcgccg ggctgggagt ttttgctggc tgcgtgggca agagcgctca gtttccgctg
     1141 cacgtctggc tgccggacgc catggccggt ccgaccccgg tctcggcctt gattcacgcg
     1201 gcaaccatgg tggccgcggg ggtgtatctg gtcgggcggt tctttccgct ttttacgaat
     1261 gacgtgctac tttacatcgc ctacaccggg ggtatcacgt tattcatcgc agcgaccatc
     1321 gccatggtcc agaccgacta caaaaaagtg ctcgcttatt cgacggtcag tcagcttggg
     1381 ttcatgatgc ttggtctagg cgtcggggga tgggcagcgg ggctctttca cttgttgacc
     1441 catgcattct tcaaggccct tttattcttg ggtgccggca gcgtgtatca cagcgtccac
     1501 acctatgaaa tgcccccttt gggtggtctc ttgaagaaga tgccgatcac ggcctggacg
     1561 atgctcgtcg gcacgctggc gatctcgggc gtacccctct tcagcgggtt ttactcgaag
     1621 gacgcaatcc tggccgccgc gttggcgcgg gtcataggta gccccgaaca tatactgctc
     1681 tttctgtttc cggcggtcgg cgcgatcttg actgcgtttt acatgttccg gatgtggttc
     1741 ttgacgttcg ccggcgagcc gcggggtttc acgtcgaagg tcgaggcggt ccactcgcac
     1801 gtccacgacg aggagcacgc gcttgacaaa gatcatggcg tcggccatgg gcatggccac
     1861 ggccacgacc tcaatccggc cgcgcacgct cacgagagcg agccgatcat gacctggccg
     1921 ctcattatcc tggcggtatg cagcgttttt gccggctgga cactgtttta tatcatccca
     1981 cttcccttcg gcacgccaac cctcgagcac atactcgaat acggtgaacc ctaccgggcc
     2041 acggatatcc tggctatgca ccattattat gcgtttggcg catcgctcgt gatcatgctg
     2101 acggggatcg ggctgggatt actctattac gctccgcacg gcttccctta ctttgtgccg
     2161 actcgtttca gtgcggtccg cacggccgag cggtttggag gtgtttatcg cttcctggtc
     2221 cataaatggt actttgacga gctctactgg gctgtgttcg tcaggccctg cttggccctg
     2281 gcccggttgt gccgccagct cgatcaggtc ttggtcgatg gcctggtcaa tgcatcggcg
     2341 tcggccacgg cgttcctcag tcggcgcgga ggttggttcg acttgatcgg cgtcgaccag
     2401 atcgtcaacc tgacagccaa agccgtatac gtgaccggcg actggagccg ttcgattcaa
     2461 accggcaaat tgcgtaacta cctgatgttt ctgtccgtca tgctggtggt cctgtttgcc
     2521 ggcgtgctcg tttggatccg cggttaggac gcgttccgcg atactttgac ctgaccctcg
     2581 caccggcaat ctctcgatct gccatcagcc ctgaattggt aaccgcccca tgagcgacaa
     2641 cctgcttttg acatccctct ggctcgtccc cttgatcggc ttggtgaccg tgctcatttt
     2701 gcccaaaccc gccgagcaag cggtcaaatg ggtctcgctg ggcttcacgt cattgacctt
     2761 cttgatcacg cttgtggtcc tgggtattta cctgggcgag ggtctcaagg acaagtcgct
     2821 gcgcgaacgc gtcgtcaaca acaagcttgt ttcgtcggca ggaatggagc tgaccgaaag
     2881 cgatgagacg gggggagagt ccgacctggt ggtccggcgc gcttggattc cctacttcaa
     2941 tattcagtac tacctgggtg ttgacgggat cagcttgagc ctggtcgtgc ttaccggact
     3001 ggtgagcgtg ctcgcgtgcc tggcgtcgtg gaatatcgaa aagcaggtca aaggctattt
     3061 cgcgctctat ctcctgttgt gcgcgagcat gatcggcgtg ttcatctcgc tcgacctgtt
     3121 cctgttttat gtctttttcg aggtgatgct gctgccgatg tacttcctga tcgcgatatg
     3181 gggaggacca agaagagagt acgcggcaat taagtttctt ctctatacgc tcttcgggtc
     3241 cgttttcatc ctggtcgcga tcctgattct ctatttctgg cagagcggcg tgaccgtccc
     3301 aggcttctcg ggacattcgt ttgacatcgt acagcttacg accgtggcca gcgagacgag
     3361 ctactatccc cggtcgatcc agtggtggat atttatcctg tttttcaccg gcttcatcgt
     3421 caagctgccg tcgtttccgt tccacacctg gctccccgat gcgcacgtcg aggctccgac
     3481 gccgatcagc atgattctgg cggcaatcct gctcaagatc ggtggctatg ggctgatccg
     3541 tctggcgtgg ccgctggcgc cggccggagc ttacgactgg tcggtgtggg tcggcatcct
     3601 gggtgtgttc agcatcattt acggcgcgct cgtggccatg gcccagaccg atttcaagaa
     3661 gcttgtcgcg tacagttcgg tcagtcacat gggctacgtc accctgggaa tggccgtcat
     3721 gggaatcttc gcagccgacg gttccaggct c
//
LOCUS       DATAQL010000328         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5222739, whole genome shotgun sequence.
ACCESSION   DATAQL010000328 DATAQL010000000
VERSION     DATAQL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5222739"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VHS97_05590"
     CDS             complement(<1..221)
                     /locus_tag="VHS97_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447703.1"
                     /translation="MRSANRRGWSVWLGWHADGHINGEASYASACLNPVSRGGHRIPR
                     RARALEILMLLWGFGDVGPGVCSMIDWKG"
     gene            complement(352..789)
                     /locus_tag="VHS97_05595"
     CDS             complement(352..789)
                     /locus_tag="VHS97_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447704.1"
                     /translation="MRTNNVSGKCVSLGAFNLLVDRCERGVRVTAVTQQSDLSGAVFL
                     DRLRQQKIADNSSGFRGLARGAFRMKEGEGIANLMRFSDIEIPEFVEHVVREFPDDCV
                     LPDDRDFDRSVIVSTDVQATEQYQLAQARKVVRMYRAWEAAQN"
     gene            complement(1086..>2172)
                     /locus_tag="VHS97_05600"
     CDS             complement(1086..>2172)
                     /locus_tag="VHS97_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX3447705.1"
                     /translation="DVIAARMEHQVVIYDTPQVLTAIRAAASRELGLMTKWRPRFGFT
                     PWVDEVRPAAVEIDAGDVTPEVCREFKSRGIKVQAKTLGADDRPEVWDRMAEVLVDWI
                     QTDFAEEVIARQIFKTIGSKPVKIAHHRGASRYAPENTLPSLEKAIRLGADFVEFDLQ
                     TTRDGGFVLLHDRSLNRTTNGRGLAREWDLAKIQALDAGSWFGRQFALTPVPTFDEFL
                     KAAGGRVELYVDAKDIAPEALAETLKRHGLIERSVVYQSAAYLEKLRAIAPTIRRMPP
                     LRAEASLDSVAEQVQPYAFDTNWSILSKELIDRCHARGIKVFSDALGSHERVEDYQQA
                     IRDGIDLIQTDHPVRVLRAIELLDQRK"
BASE COUNT          419 a          671 c          630 g          452 t
ORIGIN      
        1 atgcctttcc agtcaatcat cgaacacact ccggggccaa cgtcgccaaa accccagagc
       61 agcatcagta tttctaatgc ccgggcccgt cgaggaattc gatggccgcc ccggctaacg
      121 gggttgaggc acgccgacgc gtacgatgct tcgccattga tgtggccgtc ggcgtgccac
      181 cccagccaga cactccagcc tcgtctgttg gctgagcgca cacattattt gctcgatcgc
      241 tgcttgtgat aataattgtc cgcaattgtt ctaaaaaatc aacaatgcgg aaaagcaaat
      301 cccgcgcgac atggtgtggt ggtcggccgg aatgtagaaa agctcttccg atcagttttg
      361 agcggcctcc cacgcgcggt acattcgcac gaccttgcga gcctgagcca gttgatattg
      421 ttccgtggct tgaacgtcgg tcgacacaat cacggatcgg tcaaaatccc gatcatccgg
      481 cagaacgcag tcgtcgggga actcccgcac gacatgctcg acgaattccg ggatttcgat
      541 gtcgctgaag cgcattaggt tggcgatgcc ttccccttct ttcatacgaa atgctccacg
      601 ggcaagtcct cggaaacccg acgaattatc ggcaattttt tgctgccgaa gccgatcgag
      661 gaagaccgcc ccgctcaggt cggactgctg ggtgacggcc gtcacgcgga ctccgcgctc
      721 gcacctgtca accagcaggt taaatgcgcc aaggctaacg cacttgccgc tgacgttatt
      781 agtgcgcacc gacgtcgttc attatcgctt gacgatggga cgcccatttc cctgttatct
      841 atcctgctgc cgccgacaaa acgtcacgag cggctgttgg tttttctaga gacgacccgc
      901 tatgtatccc gcgcaagagg cggcgggcaa acaacatcct gatcgccatc gatcaaactc
      961 cggtcggtgt atcgaggttg ccgagatcac aaccccggca cgaccacggt ccgtcgggag
     1021 tgtcggatgt cagcgcagcc ggctggatcg tctcaagcgc ctctaaatcg atgtcccggc
     1081 cgccactact tccgctgatc gagcagctcg atcgcacgaa gcacccgcac ggggtggtca
     1141 gtctggatca agtcgatacc gtcgcggatc gcttgttggt aatcttccac tctttcatgc
     1201 gagcccaacg cgtcggagaa taccttgatt cctctggcat gacaacggtc gatgagctct
     1261 ttggaaagga tcgaccagtt ggtgtcgaac gcgtaaggtt gtacttgctc ggccacggaa
     1321 tcgaggcttg cctcagctcg tagcggaggc atccggcgaa tagtcggggc aatcgcccgc
     1381 agcttctcga ggtaggccgc gctttggtag acgacggatc gctcgatcag cccgtggcgc
     1441 ttcaatgtct cggcgagggc ctcgggggcg atgtccttgg cgtcgacgta aagttcgacg
     1501 cgcccgccgg ccgctttgag aaattcgtcg aaggtcggca cgggcgttaa cgcaaactgt
     1561 cggccgaacc aggaaccggc gtcgagcgcc tggatcttgg ccaggtccca ttcccgggcc
     1621 agccctcgcc cgttcgtggt tcggtttagg ctgcgatcgt ggagcaagac gaacccaccg
     1681 tcgcgggtcg tctgcaggtc gaactccacg aaatcggccc cgagccggat cgctttttcg
     1741 agcgagggca aggtattctc gggtgcgtac cgcgaggctc cgcgatggtg ggcgatcttc
     1801 accggtttcg agccgatcgt cttgaagatt tgccgcgcga tcacctcctc ggcgaaatcc
     1861 gtctgaatcc aatccacgag gacctccgcc atgcggtccc aaacttcggg ccggtcgtcc
     1921 gctccgagcg tcttggcctg aaccttgatc ccgcgacttt tgaactcgcg gcaaacctcg
     1981 ggagtgacgt cgcccgcgtc gatctcgacc gcggcgggtc gtacttcatc gacccatggc
     2041 gtgaagccaa agcgagggcg ccactttgtc atcagcccta gttcccggct cgcggccgcg
     2101 cggatcgccg tgagcacctg gggggtgtcg tagatgacga cctggtgctc catcctggct
     2161 gcaatcacgt cg
//
LOCUS       DATAQL010000329         4481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4153838, whole genome shotgun sequence.
ACCESSION   DATAQL010000329 DATAQL010000000
VERSION     DATAQL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4153838"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3407)
                     /gene="rpoB"
                     /locus_tag="VHS97_05605"
     CDS             complement(<1..3407)
                     /gene="rpoB"
                     /locus_tag="VHS97_05605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX3447706.1"
                     /translation="MPTPTSVRRIIPAKKRNFGRIFDEFPVPDLTQIQTRSYERFLQA
                     DLPDDKRGDAGLEGVFREIFPIESYDKTLKLEYLHYDLGKPRYDPDECRQLRLTFGRP
                     LHVWLRLNKGEITLEESVYLGDMPIMIGGGEFIINGAERVVVSQLHRSPGVDFVVEIE
                     SSDKKLHACRVIPERGSWIELQVTKKDTLGVRIDQSGKFSSMTLLRAMSPQYSSDEAI
                     LQAFYEVEEIDSADSKAASKLEGRIACGDIVDPNTGEVLIDSGTTISKTTAQVLADAG
                     NLGTILVLKDSRDPLILQSLQEDPTTDHESALLRIYQRLRPGNPPQLEKARELFHEKF
                     FDTNRYRLGKVGRFRINRKFNQDIPEDRMTLAPEDYVNAIRYILNLRRGKGHIDDIDH
                     LGNRRLRTIDELASDELRKGFLKLRRTVQERMSLKDAEDMTPRSLINPKSISAAIEYF
                     FGRGELSQVVDQTNPLAQLTHERRLSALGPGGLNRKRAGFEVRDVHISHYGRICPIET
                     PEGTNIGLISSLGIYGGVDEYGFLITPYRKIKSGKTTDTVVWMRADEESEAYLAPADT
                     PVDDHGKLKGSILTARYQTDFVTVSLDQIQYMDISPKQMVGVSAGLIPFLEHDDANRA
                     LMGSNMQRQAVPLLISEPPIVATGLERSVAINSGMIVKALEDGTVTYVDSTRIILDHN
                     HIHKLRKYVGLNERTCLNQKPVVQVGQKVKKGEILADGASTYKGELALGRNVLVAFMA
                     WDGYNFEDAIIISEKLVRDDVYTSIHIEEFEIEIRETKLGREEFTRDIPNVSEKALRN
                     LDDNGVVQIGTYVKPGDVLVGKVAPKSKSELTPEEKLLHAIFGRAGEDVKNDSLEVPS
                     GVEGIVINTQRFSRRMSLSEEERKAFEKELKDTEAVENVRIADEYKQMIKVLEDILGG
                     PVTDPGTGKPLGRAKDPKDLAEESERFKLDALDIRSPDAVAKARETVRQYAPRIEALK
                     DEKERRINSLKRGDELPSGVLQMVKVYIATKRVISVGDKMAGRHGNKGVIAKILPEED
                     MPFLEDGTGVEILLNPLGVPSRMNVGQILETHLGWAAAKLGFQAICPVFDGASEDTIR
                     QCLKDAGLPENGKAVLYDGRTGDKFDQRVTVGYIYMLKLHHLVDDKI"
     gene            complement(3765..4175)
                     /gene="rplL"
                     /locus_tag="VHS97_05610"
     CDS             complement(3765..4175)
                     /gene="rplL"
                     /locus_tag="VHS97_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX3447707.1"
                     /translation="MSTAESTAFADTIKNLGDSLVNLKVLEVLQLTKYLKEAHGLEAA
                     AVAVAPAGPGGGGAAAEPAPAAKTEFDVVLEAIGPNKIQVIKVVRAATTLGLKEAKDL
                     VEAAPKEVKTGLSKEDADKLKKELEDSGATVKIK"
BASE COUNT          780 a         1353 c         1360 g          988 t
ORIGIN      
        1 tgaatcttgt cgtcaaccaa gtggtgaagt ttcagcatat aaatatatcc aaccgtgact
       61 cgctggtcga acttgtcgcc ggttcggccg tcgtagagga ccgccttgcc gttttcgggc
      121 aagccggcgt ctttcaagca ctggcgaatc gtatcttcgc tggcgccgtc aaagacaggg
      181 cagatggcct gaaatcccag cttggcggcg gcccaaccca ggtgggtctc caagatctgg
      241 ccgacgttca ttcggctggg aacgcccaac ggattcaaga ggatctcgac gccggtgcca
      301 tcctcgagga agggcatgtc ctcctcaggg aggatcttgg cgatcacccc cttgttgccg
      361 tgccggccgg ccatcttgtc gccgacggag atgacccgct tggtggcgat atagaccttg
      421 accatttgca ggacgcccga ggggagctcg tcgccccgtt tgaggctgtt gatccgccgc
      481 tctttctcgt ccttcaaggc ctcgatccgg ggcgcgtact gtcggaccgt ctcccttgcc
      541 ttggcgacgg cgtccgggct gcggatgtcg agagcatcaa gcttgaaccg ctcgctctcc
      601 tcggccaagt ctttgggatc cttggcgcgg cccagcggct tgccagtccc tggatcggtg
      661 accggcccgc ccaggatgtc ctcgagtacc ttgatcatct gcttgtactc gtcggcgatc
      721 cggacgttct cgacggcttc cgtgtccttg agctcctttt caaacgcctt gcgttcctcc
      781 tcgctcaggc tcatccgccg gctgaagcgc tgggtgttga tcacgatccc ctcgaccccc
      841 gaaggaacct cgagcgaatc gttcttgaca tcctcgccag cgcggccgaa gatcgcgtgc
      901 aacagctttt cctcgggcgt cagctcgctc ttgctcttgg gagcaacctt gccgaccaag
      961 acgtcgccag gcttgacgta ggtgccgatc tgcacgacac cattgtcgtc aagattacgc
     1021 agggctttct ccgagacatt cggtatgtcg cgggtgaatt cctcgcggcc cagtttggtt
     1081 tcgcgaatct cgatctcgaa ttcctcgatg tgaatcgacg tgtacacgtc gtcgcgcacc
     1141 agtttttcgc tgatgatgat cgcgtcttca aagttgtagc cgtcccaggc catgaacgcg
     1201 accagcacgt tgcggccgag tgccagctct cccttgtaag tagacgcacc atcggccagg
     1261 atttccccct tcttgacctt ctggcctacc tgaacgaccg gtttctggtt caagcaggtc
     1321 cgttcgttca agccgacata cttgcgcagt ttgtggatat ggttgtggtc gaggatgatt
     1381 cgcgtggaat cgacgtaagt gacagtcccg tcctcgagag ccttgacgat catgcccgag
     1441 ttgatcgcca ccgaccgctc gagcccggtt gcgacgatcg gtggctcgga aatcaagagc
     1501 ggcaccgcct gcctctgcat gttcgagccc atgagcgcgc ggttggcgtc gtcgtgttcg
     1561 aggaacggga tcaagccggc cgagacgccg accatctgtt tcggcgagat gtccatgtac
     1621 tggatctggt cgagcgagac ggtgacgaag tcggtctgat aacgggcggt caagatcgag
     1681 cccttcaact tgccgtgatc gtccacgggc gtatcggccg gcgccaggta ggcctcgctc
     1741 tcttcatcgg ctcgcatcca gacgaccgta tcggtcgtct tgccgctctt gattttgcgg
     1801 tagggggtaa tcaaaaagcc gtactcgtcg accccgccgt agatgcccag cgagctgata
     1861 agcccgatgt tggtcccttc cggagtctcg atcggacaga tccggccgta gtgtgagatg
     1921 tggacgtcgc gcacctcgaa gccggcccgc ttgcggttga gtccgccagg acccaacgcc
     1981 gaaagccggc gctcgtgggt caactgagcc agcgggttgg tctggtcgac cacctgcgag
     2041 agctcgccgc ggccaaagaa atattcgatc gccgcgctga tgctcttggg gttgatgagc
     2101 gaccgaggag tcatgtcctc ggcgtccttg agactcattc gctcctgaac ggtgcggcgg
     2161 agcttcaaga agcctttgcg caactcgtcg gaggccagtt cgtcgatggt gcgcaaccgg
     2221 cggttgccga gatggtcgat gtcgtcgatg tgccccttgc cgcgcctcag attcaagata
     2281 tagcggatcg cgtttacgta gtcttcgggc gccagggtca tccggtcttc gggaatgtcc
     2341 tgattgaatt tgcggttgat ccggaaccgg ccaaccttgc ccaggcggta gcggttggta
     2401 tcgaagaact tctcgtggaa gagctcgcgg gctttctcca gttgcggcgg attgcctggc
     2461 cgcaggcgct gatagatccg caacaaagcg ctctcgtgat cggtggtcgg atcctcctga
     2521 agcgactgga gaatcagcgg atcgcgggaa tctttcagaa cgaggattgt gcccagattg
     2581 cctgcgtcgg ccaaaacctg cgccgtggtt ttggaaatcg tcgtgccgct atcgatcaaa
     2641 acttcgccgg tgttagggtc gacgatatcg ccgcacgcga tacgtccctc gagcttggag
     2701 gccgccttgg aatcggccga gtcgatctct tcgacctcgt aaaaagcctg caggatggct
     2761 tcgtcgctcg aatactgcgg gctcattgcg cgcaagaggg tcattgacga gaacttgccg
     2821 gattggtcga tccgcacgcc cagcgtatct ttcttcgtga cctggagctc tatccaactg
     2881 ccgcgctcgg ggatcacccg gcatgcgtgc agctttttat cgctcgactc gatctcgacg
     2941 acaaagtcca cacccggaga acggtggagc tggctgacga ccacccgctc ggcgccgttg
     3001 atgataaatt cgcccccgcc gatcatgatg ggcatatcac ccagatagac cgattcttcc
     3061 agggtaatct cacccttgtt gagccggagc cagacgtgga gcggcctgcc gaaggtcagg
     3121 cggagctggc ggcactcgtc cgggtcgtaa cgcggcttgc ccaggtcgta gtggaggtat
     3181 tccagcttga gcgtcttatc gtagctttcg atcggaaaga tctcgcggaa gaccccttca
     3241 agcccggcat cgccccgctt gtcgtcgggc aagtcggcct ggaggaatcg ttcgtagctt
     3301 cgcgtctgga tttgcgtcag gtccggaaca ggaaactcgt cgaagatccg gccgaagtta
     3361 cgctttttgg ccggaatgat tcgccgcacg gacgttgggg tgggcatagg atcgcgctcc
     3421 tcatgggcag aagagaatgc gggttggcgc ggtggtcaat cgtccctgga ccgcacttgc
     3481 gcgccgagat actccgcggc cgccaatcac gctggtcgaa ctcggagcaa ggcgagacga
     3541 aatcggagta ggaaagccaa atgcagcctg tctcgcgcgg tcggctccag gaatagatgc
     3601 cggcccccga aaccgccgcg tccgccccgg ttggggggcg gactgggtca tggcctcatg
     3661 cacgtgccgc ggcccgtgag agagccgccg cggcacttct gactccggaa ccgaccaaga
     3721 gtgcttgtgt cggcccggcg gtctcttatg ttcgtgcgcg aacgtcactt gatcttgaca
     3781 gtcgcgcctg agtcctcgag ctctttcttc agcttgtcgg catcctcttt ggaaagaccg
     3841 gtcttgacct ccttgggagc ggcttccacc aggtctttgg cttctttgag ccccagggtc
     3901 gtcgccgcgc ggaccacctt gatgacctgg atcttgttgg ggccgatcgc ttccaagaca
     3961 acgtcaaact cggtcttggc agcaggggct ggctcggcgg cggcgccgcc acctccggga
     4021 cccgccgggg caacggcgac cgccgcggcc tccagtccgt gagcctcttt gaggtacttg
     4081 gtcagttgca gcacctcgag aaccttcaag ttgacgagag agtcgcccag attcttgatc
     4141 gtatcggcga acgcagtcga ttccgcggtg gacattggtc aaagatcctt tcctggtaac
     4201 gcttcgtaag gtctggagtc gtcaaaattg agcgcccgat ccgtttgccg gtctcagccg
     4261 acccgatcag gctgatcgtc gtttttcgtt ccgttcgatt cacgtcgtct tacaggctgt
     4321 ccataggcaa taaagcgaat agcattgatc ccgcgcgtca cggggcctct gccggcggcg
     4381 gcgtgccttc tgcgcttgct tcctgccgcg atttggcgag ggcctcgagt tgcccgacca
     4441 gcgttgaccc cggcgtcgac aaaagcgata gtgcctctcg g
//
LOCUS       DATAQL010000330         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4154038, whole genome shotgun sequence.
ACCESSION   DATAQL010000330 DATAQL010000000
VERSION     DATAQL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4154038"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2867)
                     /locus_tag="VHS97_05615"
     CDS             complement(<1..2867)
                     /locus_tag="VHS97_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447708.1"
                     /translation="MAHPESRETPARQADASRTRTERPGNRLSIVEYKLAWLSPDKLK
                     AIHFLGMQPTAAMDDLDVARSFLACFVIRGNTDEPFDRRNKHCKSDGSRAEDYGMLPQ
                     PFARSAARRQGMVRSDEPESRRIHKSCTEVIMEFAPRMLLVLIVLVTSPIMGTRAAAQ
                     ARQAAGSLTGSAARSTALWVGQDGQDWTGAGPSVGPDGLQDVHIQLSNLPAGVVLKAT
                     RIEAPGGAIWESGTNPRLLPNAELIRGSKNPSEGALFFQPDRDLKGQRLKVMVAYQDD
                     KIESVPLVAGPCDPKLRMPESPLPELSEGSLKVTWLGQDGTPGGAPGDIHVALSGLPA
                     TFTMIGAVLTDAVRGTWIERTHKSVSIPDEPSALPLSVKRRADGVAADLFFPPYRDDS
                     RETMTLRLIGADGRALIARFAGGNCDLRLRAPQPAASRILAKPGDDLQALVEKFGTVV
                     LTGGTFRLHRPLLLKRPVNLTTAADVSATLLFDQDKAGPAWTAAIKVLCSNITLDGFA
                     VRFAGPVRWNSEVAWGPAVIGMTDNFDPGYGEARQNFAFTHLDLEIPPIEKQEGWVEA
                     LRLMRLIRAQDGVIAGNVLRGGPIEFFEGPWRVEDNEFRGTIPGTFSHGVFTGHGTYD
                     LLVRGNRASSPAGSGKTWRFLVLTWQGTGDVVDHNTIEGIGSRDDDTIPWSNEPEIIL
                     TEAYHIRYEGKVIALSADGRIVRTGRPQGIAARSGDVISLLNGPAKGQWRRIVQTIDD
                     STYLVDPPIPAGTTVVSVSQGFQREVFEDNRIDMRGGRRSFGLVLVGNHFGTRVVDNH
                     ILGGECAFKLTACPTETPVTWGWTHNPFLGGVVEGNIIEDVPHGGILGLEHDPRYIKS
                     NKGRTYMSVRLDNNIVRWTGPFLGRMGVEGPKAELAGLTLGYPQSTDPGELVVRAQGN
                     RLEAPATGRNKPSLLINGADYNQRLLSNHPLRLPTAGSTVPA"
BASE COUNT          547 a          972 c          895 g          559 t
ORIGIN      
        1 gctggcacgg tcgatcccgc cgtggggagc cggagcgggt ggttggacag cagccgctgg
       61 ttatagtcgg cgccattgat caagagcgaa ggcttgttcc ggcccgtggc cggggcctcg
      121 agccggtttc cctgcgcgcg cacgaccagt tcgcccgggt ccgttgactg cgggtagccc
      181 agggtcaagc ccgcgagctc ggccttcgga ccctctaccc ccatccggcc caagaagggc
      241 ccggtccagc gcacgatatt gttgtcgagc cgcacagaca tgtacgtgcg ccctttgttc
      301 gacttgatgt accgcgggtc atgctcgagt cccaaaattc caccgtgcgg gacgtcttcg
      361 atgatattgc cctcgaccac cccccccagg aacgggttgt gcgtccatcc ccaggtgacc
      421 ggagtctcgg tcggacaggc cgtcaatttg aatgcgcatt caccccccag gatgtgattg
      481 tcgaccaccc gggttccgaa gtggttgccg accaagacga ggccgaacga ccgccggccg
      541 ccccgcatgt cgatccggtt gtcctcgaag acctcacgtt gaaatccctg ggaaaccgac
      601 accaccgtag tgccggccgg aatgggaggg tcgaccagat aggtcgagtc atcgatcgtc
      661 tggacgatgc gccgccattg gcccttggcg gggccgttca agagcgagat aacgtcgcca
      721 ctgcgcgccg cgatcccctg aggacggcca gtgcgcacga tccgcccgtc ggccgacaat
      781 gcgatgacct tcccttcata gcggatgtga taggcctcgg tcagaatgat ctccggctcg
      841 ttgctccagg gaatcgtgtc gtcatcgcgc gacccgatcc cttcgatcgt attatgatcg
      901 acgacgtctc ctgtcccctg ccaggtcaga accaaaaacc gccaggtctt accactcccg
      961 gcgggcgacg aggctcgatt tccacgcacc aagaggtcat acgtcccgtg ccccgtgaag
     1021 actccatggg aaaacgtacc agggatcgta ccgcgaaatt cgttgtcttc cacgcgccac
     1081 ggcccctcga aaaactcgat cgggccaccg cggagcacat tgccagcgat gactccgtct
     1141 tgggcccgta tcagacgcat caagcgcagc gcctcgaccc agccctcttg cttttcgatc
     1201 ggtggaatct ccagatccag atgcgtgaac gcgaagttct gtcttgcttc gccgtagccc
     1261 gggtcgaagt tgtcggtcat gccgatcacg gccggtcccc aggcgacctc gctgttccag
     1321 cgaaccgggc ccgcgaaccg gaccgcaaag ccgtcgagtg taatattgct acagagcacc
     1381 ttgatggccg cggtccacgc gggccctgct ttatcctggt cgaagaggag ggtcgcactc
     1441 acgtccgcgg ctgtcgtgag attcacgggg cgcttgagca agagcggccg gtgcaggcgg
     1501 aacgtaccac ccgtcaggac gaccgtcccg aacttttcga ccaaagcctg aaggtcgtcg
     1561 cccggcttgg ctagaatccg gctcgccgcc ggctgcggtg ccctcaaacg caagtcgcaa
     1621 ttccccccag caaatcgagc aatcaaagcc cggccgtccg ccccgatcaa tcgcaaagtc
     1681 atcgtctcgc gactgtcatc gcgatacggc ggaaagaaca ggtcggccgc gacgccgtcc
     1741 gcccgccgct ttaccgagag cggtaatgcg ctcggctcgt ccgggataga cacgcttttg
     1801 tgcgtccgct cgatccacgt cccgcgcacc gcgtcggtca gtacggcccc gatcattgta
     1861 aaggttgcgg gtagaccgct aagcgccaca tggatgtcgc ccggcgcgcc ccccggcgta
     1921 ccgtcctgac ccagccacgt gactttgaga gatccttcgc tcaactcagg caacggtgac
     1981 tcgggcatcc gcagcttggg atcgcacggg ccggcgacca ggggcacgga ttcgatcttg
     2041 tcatcttgat aggccaccat cactttcaag cgctgccctt tcagatcgcg gtccggctga
     2101 aagaagagcg cgccttccga cggatttttc gagccgcgga tcagctcggc attgggtaaa
     2161 agcctggggt ttgtgccaga ctcccagatc gcgccgcccg gagcctcgat gcgtgttgct
     2221 ttcaagacca cgccggcggg caaattgctc agttgaatat gaacatcctg gagaccgtct
     2281 gggccgaccg agggcccggc accggtccag tcttgtccgt cctggccgac ccagagggcc
     2341 gtagatcggg ccgctgaacc cgtgagcgag ccggctgcct gacgagcctg cgctgccgct
     2401 cgcgtgccca tgatagggga cgtcaccagc acgatcagaa ccagcagcat ccgtggcgca
     2461 aattccatga tgacctccgt gcatgatttg tggatccgtc ggctctcagg ctcgtcggac
     2521 cggaccatcc cctggcgccg ggccgccgag cgagcgaaag gttggggcag cataccataa
     2581 tcctcagcgc gcgaaccgtc agatttgcag tgcttgttcc ttcgatcaaa cggctcgtcg
     2641 gtgttcccgc gaatcacgaa gcatgcgagg aagctgcggg cgacgtccag atcgtccatg
     2701 gcggcggtgg gctgcatgcc caaaaagtgg atggccttga gtttgtcggg cgagagccag
     2761 gcgagcttgt attccacgat tgagaggcgg ttgccgggtc tttcggttct ggtacgcgac
     2821 gcatcggcct gccgcgccgg cgtttcccga gattcaggat gcgccaatta ttccagcgac
     2881 atgcgcaact gcaagtgacc catttgtttg gttccgaaag cggaatttcg ccaacgataa
     2941 atgtgtgcgc gctcctgggc cgcttcaatc ccc
//
LOCUS       DATAQL010000331         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4917572, whole genome shotgun sequence.
ACCESSION   DATAQL010000331 DATAQL010000000
VERSION     DATAQL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4917572"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1891)
                     /locus_tag="VHS97_05620"
     CDS             complement(29..1891)
                     /locus_tag="VHS97_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX3447709.1"
                     /translation="MKYDFLAYLWRSETWIVGSAVVAFLMATWVMRGAPPGQAAESES
                     DSDAPTARYRDRIVAAVVVGLILILLGAFVAITRGIPWSLPIFGVGLGLVLMLIAYNR
                     RYRHASPSLRRTIEFSSAFLNATLLAGILIVGNIIAFRYGGHLIDVTREQTYSLSSMT
                     LNQLKSLEQPVTFMMVFGRGPRAVRQRDRVVQLLESYKAANPAMIELDSLDPFSDPTR
                     YDELVKRLPELELLHGGGVVSGGGVVIEHGAGENAQHAVVRNGDLFQQPPSGAAQGGM
                     DQYASAFTGEDEITSALIRLREGKKAKVVFTTGHAEPLTSDLNPRGVGIGNWKARLSK
                     VGCEVIDLNLIQDEIPADTSLVVVVGPKSPFKPDEEPKLRAFAERGGPVLLLLGNTEP
                     SGLDEFLKSFNLALGGGLVIDPRFNYNRNPSLVFAPTGGTVKHPVIDPLGPNRAALLP
                     SAAPIRVFGQSMRGQAPSEPVNPKLLPVPILQTTNYSWAESNPKNPPLRFDRTADELG
                     PLTVGVAVSERPDKPVSGNATAGKPRLVLISSPNMAENVIQEIEQTNLDLLMNAASWL
                     RNRPDTQGIPPHTHVALTLAVDPFLRSRLILVPSAVAVMLIIALGIMVYVARRQ"
     gene            complement(1923..2687)
                     /locus_tag="VHS97_05625"
     CDS             complement(1923..2687)
                     /locus_tag="VHS97_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3447710.1"
                     /translation="MRHVTALLSRELGAYFLSPMAFLVLLAVQLIAFLNFWELVDTLS
                     QPQRELSSLRDPMTTYISGSPAFWIAILVAVPMLTMRLLAEERRSGTIETLLTLPVTE
                     TEIVVAKWLGGVLMFCMLLVPFALYLPFLYYQAKFNFDIGPLLTLFVGLTTMGMMMIA
                     IGLFMSSLTRNQIVAAIWTFVVLFLMVVLIPLVFSFAARQHMPWAEGVRFMAILYQIQ
                     NFSLGQLDLRYLALHLSVCVFMLFLTVKVLETRTNR"
     gene            complement(2690..3640)
                     /locus_tag="VHS97_05630"
     CDS             complement(2690..3640)
                     /locus_tag="VHS97_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3447711.1"
                     /translation="MIKVDRLTKRYGSFKAVESVRFEVGRGEIVGLLGPNGAGKTTTM
                     RMLTTYLSPTSGRASLAGHDVLDEPLEVRRKVGYLPENVPLYPEMRVIEYLRHRARLK
                     DVPRPDRRRAIAEVVSRCQLGEVERRIIGQLSRGFRQRVGLAESMLHDPEILILDEPT
                     AGLDPLQIREVRDLIRELGERHTILLSTHILSEVEAVCGRVIIIARGRIALDDQLANL
                     HRQSAIVLEARGSADVIKAALQTIPGVERVVVSRREGDYGWFEVHTRREEDLREAISQ
                     KLVTNGWPLRQLDLRRSSLEERFTQAVTRDTLADAQIEAD"
BASE COUNT          824 a         1180 c         1153 g          719 t
ORIGIN      
        1 aggtagagcg attgtttttc atcgttcgtt attgtcgtcg agccacgtaa accatgatcc
       61 ccagcgcgat aatcagcatc acggccacgg ctgatgggac caggatcagc cgcgatcgca
      121 agaacggatc gaccgcaagc gtgagggcga cgtgcgtatg cgggggtatt ccttgcgtgt
      181 ctggccgatt gcgcagccag ctcgccgcgt tcatgagcag gtcgaggttg gtttgttcga
      241 tctcctgaat gacgttttca gccatgttgg ggctcgagat caagacgagt ctgggcttgc
      301 ccgcggtcgc gttgcccgac accggtttgt cgggacgctc ggagaccgcc actccgacag
      361 taagcgggcc gagctcgtca gctgtgcgat cgaagcggag cggtgggttc ttggggttgc
      421 tctcggccca cgagtagttg gtggtttgca agatcggcac cgggagcaat ttgggattca
      481 ctggctcgga cggcgcctgg ccccgcatcg actggccgaa cacgcggatc ggtgccgcgc
      541 tgggtaagag cgcggcgcga ttgggcccta atggatcgat gaccgggtgc ttgacggtcc
      601 ctcccgtcgg ggcgaaaacc agagacggat tgcggttgta attgaagcgc ggatcgatca
      661 cgagcccgcc gcccaacgcc aggttgaacg acttcaaaaa ctcgtccagc cccgagggct
      721 cggtgttgcc gagcaagagc aagaccggcc ctcctctttc cgcgaacgca cgcagcttgg
      781 gctcttcgtc gggtttgaac gggctcttgg ggccgacgac gaccaccagg ctcgtgtccg
      841 ctggaatctc atcctggatc aggttgaggt cgattacctc gcaacccacc ttggaaagtc
      901 tcgccttcca gttgccgatg cccacgcctc gtggattcaa gtcgctggtg agcggctcgg
      961 cgtgtccggt cgtgaagacg accttcgcct tcttgccttc acgcaatctt atcaaggccg
     1021 acgtgatctc gtcttcgccc gtgaatgccg acgcatactg atccatgccc ccctgcgccg
     1081 cgccagaagg gggctgctga aacaggtcgc cgttgcgcac cactgcgtgc tgcgcattct
     1141 cccccgcgcc gtgctcgatg accacgccac ctccgctgac gacgccccct ccgtgcaaga
     1201 gctcgagctc cggcaagcgc ttgactaact cgtcatagcg ggtcggatca ctgaaagggt
     1261 cgaggctgtc gagctcgatc atggcgggat tcgcggcctt gtatgactcg agcaactgga
     1321 ccacccgatc gcgctgacgc acggcccgcg gaccacggcc aaagaccatc atgaacgtga
     1381 ccggctgctc gaggcttttg agctgattga gagtcatcga tgatagcgaa tacgtttgct
     1441 cacgggttac atcaataaga tgtccgccat agcgaaatgc aatgatgttg cccacgatca
     1501 ggatgcccgc caacagcgtg gcgttcaaaa acgcactgga aaactcgatc gttcggcgca
     1561 aactggggct cgcatggcgg tatcggcggt tatacgcgat caacatgagg accaggccaa
     1621 gccccacacc gaagatgggc aaagaccagg ggatcccacg cgtgatcgct acgaaggcac
     1681 cgagcaggat caggatcaat ccgaccacga cggcggcgac gatccggtct cgatagcggg
     1741 cggtcggtgc gtcgctatcg ctttcggatt ctgctgcctg gcctggcggt gcgcctcgca
     1801 tgacccacgt cgccatcaaa aatgcgacca cagccgatcc aacgatccag gtctcactcc
     1861 gccacaaata ggcgaggaaa tcgtatttca tcgcgaacga gaccctctct cggctcggac
     1921 acctaacggt tggtacgcgt ctctaagacc ttgaccgtca agaagagcat gaaaacacag
     1981 accgaaagat ggagcgcaag atatcgtaga tcgagctggc ccaggctgaa attctggatc
     2041 tggtacaaga ttgccatgaa gcggacaccc tcggcccagg gcatgtgttg ccgcgccgcg
     2101 aagctaaaga ccaaagggat caagacgacc atgagaaaca agaccacgaa cgtccagatc
     2161 gccgcgacga tttgattgcg cgtcagcgaa ctcatgaaga gcccgatcgc gatcatcatc
     2221 atccccatcg tcgtcaagcc tacgaaaaga gtcaaaagcg gtccgatgtc gaagttgaac
     2281 ttggcctggt agtagagaaa cggcaagtaa agcgcgaacg ggaccagcag catgcagaac
     2341 atgagcacgc cacccagcca tttggccacc acgatctcgg tctccgtcac cggcagtgtc
     2401 agaagcgttt cgatcgtgcc ggatcggcgc tcttccgcca acaagcgcat ggtgagcatc
     2461 ggcaccgcga ccaagatcgc gatccagaat gccgggctac cggaaatgta cgtggtcatc
     2521 gggtcacgta ggctcgaaag ctcgcgctga ggctgactga gagtgtcgac cagttcccag
     2581 aagttgagaa acgcaatgag ctgaaccgcc aagaggacca ggaaggccat cgggctcaag
     2641 aagtaagcgc ccaactcacg ggataagagg gctgtgacat gtcgcatgat caatcggcct
     2701 cgatttgcgc gtcggcaagg gtatcccgcg tcacggcctg cgtgaaccgc tcttccagcg
     2761 agctgcgccg cagatcaagc tgccgcaagg gccagccgtt ggtgacgagc ttctggctga
     2821 tcgcctcgcg caagtcctct tctcggcggg tgtggacctc gaaccagccg taatctccct
     2881 ctcgacgcga cacgaccact cgctccacgc cgggaatcgt ttgaagcgcg gccttgatga
     2941 cgtcggccga gcctcgtgct tccagaacga tcgcactttg acggtgcaga ttggcaagct
     3001 gatcgtcgag agcgatgcgg ccgcgggcaa tgatgatcac ccggccgcaa accgcctcga
     3061 cctccgagag aatgtgggtg gataagagga tcgtgtgccg ttctcccagc tctcgaatca
     3121 ggtctctaac ctcgcgaatc tggagcggat ccaggcccgc cgtcggctcg tccaagatca
     3181 agatctcggg gtcgtgcaac atcgactccg cgaggccgac ccgctgacgg aatccgcgcg
     3241 aaagctggcc gatgatccgg cgctcgacct cgccaagctg acagcggctc accacctcgg
     3301 cgatcgcccg gcgccggtcc ggtcggggta catccttgag acgcgctcga tgccggagat
     3361 actcgatcac ccgcatctcg ggatagagcg gaacgttttc gggaagatat ccgaccttgc
     3421 gccggacctc gagcggttcg tcgagtacat cgtgaccggc aagcgaagca cggccgctcg
     3481 tgggactgag ataggtcgtc agcatccgca tagtcgtcgt cttgcccgcc ccgttgggcc
     3541 cgagcagacc gacgatttcg ccacgcccga cttcaaaccg gacgctctcg accgctttga
     3601 acgagccgta gcgtttcgtg agtcggtcca ccttgatcat ggcatcgcgc acccagcagg
     3661 caaattccgt cgtcccggtc ccagtccgtt tgtcagcgga tatctaacaa taatgcctca
     3721 accgtccgtc gtgacacgaa gtgacgaaca aaacgggaat aaccgtctca caacgccagt
     3781 gtcgcacgag tacccgttgg ccaggtagat tcgcgctatg tcttgagctg gccgcagcag
     3841 attccaccgg cattggatca aagacgatgt gatagt
//
LOCUS       DATAQL010000332         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5375663, whole genome shotgun sequence.
ACCESSION   DATAQL010000332 DATAQL010000000
VERSION     DATAQL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5375663"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VHS97_05635"
     CDS             complement(<1..1420)
                     /locus_tag="VHS97_05635"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3447712.1"
                     /translation="MWVWLDRAGSILFDATLSTAIFLTLVVLAMLVCRQPTRRVLIAR
                     SALVASLAMIPLVALLPLPRFDLLDTLIQSDLLPRHLVAELERTGHSASAALATEHPS
                     HWIFANDLHFRLLGTGRWLPRSLTLIDLACVIIGVAWLSLGVWGVRWLIRHSRSPSPT
                     TLSIYEQLCGGEMIGRLRPLLRVSPRVQHPVVVGLFRPTILIPLAFDEPEGDPELLRL
                     SLLHEMAHAEQWDPWFGTIAGLAQIVWFFLPQTWWLRSQLLIDQEFMADRFAAIHYGT
                     SSGYAASLVSMAESRRGHDADHRSNGGASGWIAMAKNEIRSPLAQRVLMLLYCPFRFE
                     ATAPRSWSWILRITLAMTSIVAACLCVRWPDASAIEHRVKNGSAQSTSQFRVSDFTAE
                     PLAFSRSGRALSFVMPVALPTDFELTVEVFSALADLGNVRIAGHALGDQEDSTESLNH
                     TPGSSDMVKSWHDVRLIRRVDDV"
     gene            complement(1668..2099)
                     /locus_tag="VHS97_05640"
     CDS             complement(1668..2099)
                     /locus_tag="VHS97_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3447713.1"
                     /translation="MRKTQMVTDRELEIMKVLWARSRASVREVQEDLNRGAGPVAYST
                     VQTLLNIMEDKKGLVRHVVEGRTFVYIPKKSSERTIRELTSRFVDRVFDGALDRVMVA
                     LLGSKSPTPEELDRLRAMIDEAQQQSFLKPDAEEELNSQEA"
     gene            2556..4166
                     /gene="cimA"
                     /locus_tag="VHS97_05645"
     CDS             2556..4166
                     /gene="cimA"
                     /locus_tag="VHS97_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX3447714.1"
                     /translation="MTRIFIYDTTLRDGSQGEGVNFSLQDKLLITSRLDELGFDYIEG
                     GYPLSNPKDAAYFRAVRDLDLKHAKVAAFGMTRRRDVAAEDDQGMKALIGAQTSAITI
                     VGKSWDLHVRDVLGVSLEENVKMIGDSVAFCAAHVPDVIYDAEHFFDGFKRNPDYALR
                     TIEAAAAAGAAWIVLCDTNGGALPEEVALAVAQVARAISVPIGIHTHNDGDLAVANAL
                     AAVRHGARQVQGTINGLGERCGNVDLCSVVANLAFKCHGLSVLVPDKLDRLTEISRYV
                     YETANMNFRSNQPFVGSSAFAHKGGMHVHGVRKNAASYEHIDPATVGNERRVLISELS
                     GKSNIAEKLSEYGLEQDSALLARVLDRVQDLENEGYQFEAAEASFVLLVERLAERRRN
                     WFGALGFRVSVSGEPGQVSATSTEATIKLKVGGVFEHTVSEGDGPVNALDGALRKALE
                     RHYPQLKEMSLVDYKVRVINARAATAARVRVVIESKDQDDVWGTVGVSENVIEASWLA
                     LVDSFEYKLNKDARADPPARLILTESTS"
     gene            4250..>4579
                     /locus_tag="VHS97_05650"
     CDS             4250..>4579
                     /locus_tag="VHS97_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEX3447715.1"
                     /translation="MTPAEIGMSSKLARIASAEFSTYFRHLATRVERAARSVSEEQLW
                     TKPFPFGNSIGHLILHLTGNLNHYIGALIAGSGYVRHRDHEFTDPARYPLEELLARFH
                     QAVESVVT"
BASE COUNT         1023 a         1313 c         1346 g          897 t
ORIGIN      
        1 agacgtcgtc cacccggcgg atgagccgta catcgtgcca cgacttgacc atgtccgatg
       61 aaccgggagt gtgattaagc gattccgtcg agtcttcttg gtcacctagg gcatgacccg
      121 cgatgcgtac gttgccgaga tcggccaaag ctgaaaaaac ctcaactgtg agctcgaaat
      181 cggtgggaag tgcgacgggc atgacaaaag acagagcccg gccactgcgc gagaaagcga
      241 gtggttcagc cgtaaaatcg ctgacgcgaa attgggacgt tgattgggcc gagccatttt
      301 tcacgcggtg ctcgatggcg ctggcatctg gccagcggac gcaaaggcat gccgcgacga
      361 tcgaagtcat cgccagggtg attctcagga tccatgacca cgagcgggga gccgtcgcct
      421 cgaaccgaaa cggacaataa aggagcatca agacgcgctg agccagaggc gaacgaatct
      481 cgttcttggc catcgcaatc caaccgcttg ctccgccgtt agatcggtgg tctgcatcat
      541 gtccacgtcg agactccgcc atagacacaa gcgatgcggc gtaaccagac gatgtaccat
      601 agtgaattgc cgcaaatcga tcggccataa actcctgatc gatcaaaagt tgtgaacgaa
      661 gccaccaggt ctgcggcagg aaaaaccaca caatctgagc gaggccggca attgtgccga
      721 accaagggtc ccattgttct gcgtgggcca tttcgtgaag tagactcagc cggagtaact
      781 cgggatcgcc ttcgggctcg tcgaacgcga gtgggatcag aatcgttggg cgaaacagtc
      841 cgacaacgac cggatgctgg acgcgcggtg acacacgcaa cagggggcgc aatcggccga
      901 tcatctcgcc gccgcaaagc tgctcataga tggatagggt tgtgggagat ggactacgcg
      961 agtggcgaat gagccaccgc actccccaaa cgccgaggga tagccaggcc actcctatga
     1021 tcacgcacgc caggtcgatc aatgtcaggc tgcggggcag ccatcgaccc gttcccaaca
     1081 gcctgaagtg tagatcgttg gcgaaaatcc aatgggacgg atgttcggtc gccaatgcag
     1141 cggacgctga atgcccggtt cgttccaact cggcgacgag gtgcctgggt agcaggtcgg
     1201 actggatgag cgtgtccagc aaatcgaacc gcggcaacgg caaaagcgcg accagcggga
     1261 tcatcgccag ggatgccacg agggcactgc gtgcgatcaa gactcggcgc gtgggctggc
     1321 ggcagaccag catggccagg actaccaacg tcagaaatat ggccgtcgac agggttgcgt
     1381 cgaacaggat cgaccccgcg cgatcaagcc acacccacat ggtgcgaacc cttatcgaag
     1441 gacttttctc gaacgacccg tttcgaaccg attttttgcc atatcgtcgg ggtcgcagga
     1501 gcagtcgggg gcgcagaagt taattttaag aacgcgaata accccgacat ctcagtcaac
     1561 ccgtcaaggc ggagcctaca tcctttggcg gccttaccgg gctttcacag gtaccggcgg
     1621 tgactcgttt tcctctctat taactttgcg ccccagcgcg aaacagttca agcctcttgg
     1681 gaattaagtt cctcctcggc gtcaggcttg agaaacgact gttgctgggc ttcatcgatc
     1741 atcgcccgca gccgatccag ctcttcggga gtcggcgact tcgagcccag cagggcgacc
     1801 atcactcggt ccaacgcccc gtcgaagacg cgatccacga atcgactcgt caattccctg
     1861 atcgtccgct cggatgattt cttgggaata tagacgaaag ttcggccctc tacgacatga
     1921 cggaccaggc cctttttatc ttccatgatg ttgagcaagg tctgcacggt gctgtaggcg
     1981 accggaccgg ctccccggtt caggtcttct tgcacctctc gaacactcgc ccgactgcgt
     2041 gcccagagaa ccttcatgat ctccagttca cgatcggtca ccatctgagt cttacgcact
     2101 tcattctccc accttaatga tctgaaaaac gggaataatc atgcacgtaa cgtaacgtgc
     2161 tccatcacaa tacgcaaccc taagcaactc ggaattcgcg ccttttttcg cgattcacaa
     2221 cgtgtatgtg aaagaatgca acgtctcaag ctaggtggat tgcgtaaagt ggtggtcatt
     2281 gcccggactc tttcaattca cacgaaacac ctttaaaaaa aacaaagtcc aaacggtaaa
     2341 atattttgct cctcataata tcgcccgcgg tgtcagaggg ttgaaacaac aaacgaccga
     2401 taaatagcag tcatggatgg ttggccgcaa tcagcaaatg gcggtgtctg gttcgcgtct
     2461 ggctgtcgcg agttgcggcc gaccccgtta caatcaggat ctgaccgacc acaccgaaga
     2521 acctaggcgc cgccgcgggg aaacctatct gaactatgac gcgaatcttc atttatgaca
     2581 cgacgttgcg cgacggcagc cagggggagg gtgtcaattt ttcgctgcaa gacaagttgc
     2641 tgatcacgtc acggctcgac gaactgggat tcgactatat cgagggcggc tatccgcttt
     2701 ctaaccccaa ggacgccgcc tacttccgcg cggtgcgcga cctggatcta aaacacgcca
     2761 aagtcgcggc gttcggtatg acccggcgcc gcgacgttgc ggccgaggac gaccagggga
     2821 tgaaggcttt gatcggggcg cagacgagtg ccatcacgat cgtggggaag agctgggacc
     2881 tgcacgttcg cgatgttctc ggcgtgtcgc tcgaagaaaa cgtgaagatg atcggcgact
     2941 cggtggcctt ttgtgctgcg cacgtgcccg atgtgattta tgacgccgag catttcttcg
     3001 atgggttcaa gcggaatccc gactatgcac tgcggacgat cgaggcagcg gcggcagcag
     3061 gtgcggcttg gatcgttctt tgcgacacca acggcggcgc cctgcccgag gaagtcgccc
     3121 ttgcggttgc gcaggttgca cgtgcgatct cggttccaat tggaatccac acgcacaacg
     3181 acggtgacct cgccgtggcg aacgccctgg ccgcggtccg gcatggcgcg cggcaggttc
     3241 aaggcacgat caacggcttg ggcgagcgat gtggcaacgt tgacctgtgc agcgtcgtcg
     3301 cgaatctcgc gttcaaatgt catggcttgt ctgtgctcgt gccggacaaa ctcgaccgat
     3361 tgaccgagat atcgcgctat gtttacgaaa cagccaacat gaacttccgg tccaaccaac
     3421 cttttgtcgg ctcgagcgcg tttgcccaca aaggcgggat gcatgtgcac ggtgtgcgca
     3481 aaaatgccgc cagttatgag cacatcgatc cggcgactgt cggcaacgag cgccgcgtct
     3541 tgatcagcga gctgtcgggc aagtccaaca tcgccgaaaa gctcagtgaa tatggtctgg
     3601 aacaagacag cgcgctcctg gctcgcgtgt tggaccgtgt ccaggatctg gagaacgagg
     3661 gctaccagtt tgaggcagcc gaagcgtcgt tcgtgctctt ggtcgagcgc ctcgctgagc
     3721 gtcgccgcaa ttggttcgga gcgctcggct ttcgcgtcag cgtcagcgga gagcccggcc
     3781 aggtttccgc cacttcgact gaggcgacga tcaaactcaa ggtcggcggg gtattcgagc
     3841 atacggtgag tgagggagac ggaccggtca acgcgctcga cggcgcgtta cggaaagccc
     3901 tcgaacggca ctatccacaa ctgaaagaaa tgagtctggt cgactataag gtccgagtta
     3961 tcaatgcgcg ggccgccacc gccgcgcgcg tgcgggtcgt gatcgagagc aaggatcaag
     4021 acgacgtgtg gggaaccgtg ggcgtttcgg aaaacgtcat cgaggcgagc tggctcgcgc
     4081 tcgtcgactc gttcgaatat aagcttaaca aggatgcgag ggccgatccg ccggcccggc
     4141 tgatcttgac ggaatcaacc tcttgaaccg cgctgattct gacgcggccg agaatccgat
     4201 cgccggctgt tctccgtttt tcaaactgaa tacccaggag tcgaccgcca tgacgccagc
     4261 cgaaatcggg atgtcgtcga aactggcccg gatcgcgtcc gcggaatttt ccacttactt
     4321 tcgccacctc gcgacgcggg tggaacgggc tgctcgatct gtgagcgaag agcagctctg
     4381 gaccaagccg tttccgttcg gcaatagcat cggccacctt atcctgcacc tgaccggcaa
     4441 cctgaatcac tacatcggag cgcttattgc cgggtcggga tacgtgcgtc atcgggacca
     4501 tgagttcacc gacccagcgc ggtatcctct cgaggagttg ctggcccggt ttcaccaggc
     4561 cgtcgaatcg gtcgtaacg
//
LOCUS       DATAQL010000333         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1406237, whole genome shotgun sequence.
ACCESSION   DATAQL010000333 DATAQL010000000
VERSION     DATAQL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406237"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VHS97_05655"
     CDS             <1..596
                     /locus_tag="VHS97_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX3447716.1"
                     /translation="SLVSIKPSIRFDNRNSPFMPTKGQYLQLSTEQGWGSFTWTKFDA
                     EGRVYLPVYSRPDGTGKQFFTLRGHFGIATESTPVYERYFAGNFGSLRGFQYRTVSPH
                     AFDVPTGGVMMAVGSVEYQFPWNARDTFHQIFFTDFGTVTGNYELSNMRVSVGTGLKV
                     SLPMFGPMPFEFDLAFPVLKTQGDKVQTFNFSVSGSW"
     gene            789..1379
                     /locus_tag="VHS97_05660"
     CDS             789..1379
                     /locus_tag="VHS97_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447717.1"
                     /translation="MIRYFLVMAVTGTSLLVADFVVGFLAAGELHGPGAVWRPTHVLF
                     SLVTVVLLLAIHSIVYTYFVATGKWAKEVVQVYQLPDWIGTQATKNKRRAFRFIMGSM
                     TAIAVAAWLGAAADTRGPSYAIWHLGSAAFALAYNIGSFVVEYAVIVAHVRLLADLKD
                     RADRLREERYGLANVRGGGVDEQPPAAQRDSPIALP"
     gene            1435..>1991
                     /locus_tag="VHS97_05665"
     CDS             1435..>1991
                     /locus_tag="VHS97_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447718.1"
                     /translation="MRPLGVIVLIVLVDLLGFTIVMPLLGPFGAHYGFSDWQIGLLFS
                     SYQVAQLVAGPVLGRLSDRYGRRPILIFSQAGTALSFLILGLSSNFTVMLFARLLDGA
                     SGGNILVAQAYVADVTAPEDRARGMGLIGMAFGLGFVFGPLLGGLLLCLPLADTWRLR
                     VPFLVAAAFSTMAWVLVLTRLPESR"
BASE COUNT          326 a          571 c          624 g          470 t
ORIGIN      
        1 cgtcgttggt ctcgatcaag ccgagtatcc ggttcgacaa tcgcaacagt cctttcatgc
       61 ccaccaaggg acagtatctg caactctcaa ccgaacaagg ctggggtagc ttcacctgga
      121 ccaagttcga cgcggagggg cgagtgtacc taccggttta tagccgcccg gacggaaccg
      181 gcaagcagtt cttcacgttg cggggccatt tcggcattgc gaccgaatcg accccggttt
      241 atgagcggta ctttgcgggt aacttcggca gcttgagggg ctttcaatat cggacggtca
      301 gtccccatgc gttcgatgtg cccaccggcg gcgtcatgat ggccgtcggg tcggtcgaat
      361 accagtttcc ctggaacgcc cgcgatacgt ttcatcagat ctttttcacg gacttcggca
      421 cggtcaccgg taattacgag ttgtccaata tgagagtgtc ggtaggtacg ggtctgaagg
      481 tcagcctccc gatgtttggc cccatgccgt ttgagtttga cctggccttt ccggtcctga
      541 agactcaagg cgacaaagtt cagaccttca acttctcggt cagtggatcc tggtaaaccg
      601 cgcggccgtc tgagcaaggc aaagatctac ggccgcgcgc ttgcaagcca agggtaatct
      661 tctcgtgcgt tgccagcagg cggtgacctc gcttagttta gagatcgtct cggagccggc
      721 aactgggttg gttggctccg actttagttg aatacgaggc gcgaggtcgc cgggcgcagg
      781 gtgatgtaat gatccgctac ttcttggtga tggccgtcac ggggacatcg ctgctcgttg
      841 ccgatttcgt ggtcggtttt ttggccgcag gcgaactgca tgggccaggt gcggtctggc
      901 ggccaactca cgtgctattt tcgctggtga ctgtggtcct gctcttggcc attcactcga
      961 tcgtgtatac gtacttcgtc gcgaccggaa agtgggccaa ggaagtcgtt caggtctatc
     1021 agctacccga ctggatcggc acacaggcga ccaagaacaa acgacgcgcg ttccgcttca
     1081 tcatggggag catgacagcg atcgcggtgg ccgcctggct gggggccgcc gccgacacgc
     1141 gaggcccctc atacgcaatc tggcacctcg gctccgcggc gttcgcgctg gcctataaca
     1201 tcggatcctt tgtggtggaa tatgccgtga ttgttgcgca tgtccggctg ctcgcggatc
     1261 tcaaggatcg tgccgatcgg ctccgcgaag aacggtacgg actggcaaac gtgcgcggcg
     1321 gcggagtcga cgaacaaccc cccgccgccc aacgagactc accgatcgct cttccctgaa
     1381 gcgtacccat ccttccgttt tcatccttgg ttcattggag gataatcgca gccgatgagg
     1441 ccgctcgggg ttattgtgct gatcgtcttg gtggacctgc tcgggttcac gattgtgatg
     1501 ccgctccttg ggcccttcgg agcgcactac gggttcagcg attggcagat tggattgttg
     1561 ttctcgtcgt accaggtcgc gcagctggtt gcgggtccgg tcctgggtcg gctttccgat
     1621 cgctatggcc gtcgtccgat cttgatattc agtcaggcgg ggacagccct ctcgttcttg
     1681 atcttgggcc tgtcgagcaa cttcaccgtg atgctcttcg ctcggctttt ggacggtgcc
     1741 tcggggggca acatcctggt tgcgcaggct tacgtcgccg acgtgaccgc gcctgaggac
     1801 cgggcgcggg ggatgggatt gatcggcatg gcgtttgggc tgggctttgt attcgggccg
     1861 ctcttgggcg gactgctctt gtgcctgcct ctagccgata cctggcggct tcgagtgccg
     1921 ttcctggtgg ccgcggcatt ctcgacaatg gcgtgggtgc tcgttctgac gcgcctcccc
     1981 gagtcgcggc a
//
LOCUS       DATAQL010000334         8914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3239597, whole genome shotgun sequence.
ACCESSION   DATAQL010000334 DATAQL010000000
VERSION     DATAQL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3239597"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VHS97_05670"
     CDS             <1..498
                     /locus_tag="VHS97_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3447719.1"
                     /translation="IFVMGHSAGAQLAALVTIDDRYLKAEGVPFDVLKGCVPVDGDTY
                     DVPAIIETGETRRRVHGQPQPKFGHREKFGNDPEKHRIYSAVTHVAKDKGIPPFLILY
                     VAEHPDTSAQAQRLGTVLKDAGIPAKVFGAQETNHSRIDANLGTSDDPATKALFEFVS
                     KALNN"
     gene            732..1451
                     /locus_tag="VHS97_05675"
     CDS             732..1451
                     /locus_tag="VHS97_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3447720.1"
                     /translation="MRLLTAIPVYNEEASLVGVLTEVLKYARDILVVDDGSSDRTPAL
                     LRDFPTVRTIRHPRNLGYGAGLRTAFQATIDGGYDGVVTLDCDGQHEPARIPEVASHL
                     VEADIVSGSRYLRVFDPSQEPPVERRRINVEVTRWLNECLGMNLTDAFCGFKAYRTSA
                     LRLFDITDEGYAMPLELWVQAVAHRLKIMEVPVPLIYLDESRAFGGALDNAEYRLKHY
                     RRVLEKALERAGLLAPEGCLG"
     gene            1448..3115
                     /locus_tag="VHS97_05680"
     CDS             1448..3115
                     /locus_tag="VHS97_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447721.1"
                     /translation="MTARRIKAPSIDGGVLVDPNPSAIAGQIAANADRLSGWDHDFQG
                     RRAKWLRSAVRQEVVALARSFLARHGFCDSSLEIEALGRNDCPLIVTGHQPELFHPGV
                     WIKNFAAASIATANGGVGLNLIVDNDIPKSTSIAVPAVGAQGIRLTRVEFDRWGGDAP
                     FEDSPVLEEERFSTFADRVHAVMGDSIPNPLLDRFWPCALGRRPEAWTHGMRFSLARR
                     EIEESWGVMNLEVPLSEVCQSDGFYWFVSHLLAELSRYARVHNEALTEYRAMHGIRSK
                     NHPVAALATEGEWLEAPFWIWRAENPRRRPLLVRQRTNVMDLRIAGEDEVLIELPLAP
                     HREACCAVERLRELPARSVRLRTRALTTTLFSRFLLGDLFIHGIGGAKYDELGDEIAR
                     RFFQVEPPGFLTVSMTLWLGLPAEPASAADLIDVNRRLRDVQFNPDLCLSEPYDEELR
                     NLVRKKREAILQSVASRRERKVRRLAIRRANEAIRPWVRHQEDEFLGRKLEILRRLRS
                     NRAARNREFSSVLHAEDRLRSSLLEAASSSARLIETSVSSGAPGQTS"
     gene            3272..4636
                     /locus_tag="VHS97_05685"
     CDS             3272..4636
                     /locus_tag="VHS97_05685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3447722.1"
                     /translation="MKTKRGLMTRVELPRTRADGRVARRVSTMVDLMKAPAPGNRLPF
                     RRGSVEYCLATEADHEAIYQTLLHVFHGPDRESFLGALSDPVYRPDQRILAKVDGRIV
                     SHVHLTERQVRYGSVTIPISGVMWVGTLPEFRGMGFAQNLMRLASLRARENGVALQVL
                     TTGMPQFYRSIGWGICGRQSFAQTMSRNLPQATDGVIEGRGLSWQVRPWRQVELGDLM
                     SLYERQYAETTGTIVRSEEYWRWMIGRKYAHVIWVACQGETVRGYAFIKDHKVLEIAF
                     DSNYPQALRALLGRVRAEALERAYPEVTVYAPSDHPVIETVRTASGRIIDQDAYEGTV
                     SMYHIPDLGRFLGCIAPELARRATESGGTLPLELGICLEDERWLIHIDGKNSRVEREK
                     LSRRHLTLTPSALVRLLMGHTGIANASDEDGFIASTSTALDAARILFPQTAIWRSPLD
                     SATA"
     gene            complement(4713..5552)
                     /locus_tag="VHS97_05690"
     CDS             complement(4713..5552)
                     /locus_tag="VHS97_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447723.1"
                     /translation="MDKRPPTPEDLPIPISEGDLRDTFDLPLVSGGGREAERGEASVG
                     SGTADVAALFKDDGRSKRRPTAAEARTRARRCTHCGGGVPQGMSLCPTCGTDQETGMR
                     VGLEDDFAPPPPPRSDGPPLHVAIVGGLCGTAGLIFTLLGVIQSTRSESSMQNYAWLV
                     LALVSGFGIYACVQFIRGKSVKLLMLALALGVAVDVIGLIAFPIVRAMIDDPDQITRE
                     FKPIDLDDSNIGIRPFEERIDTQKIVFGVTIILVYAVLSLYLMSPPVKKYLFLARGER
                     DVY"
     gene            6601..8139
                     /locus_tag="VHS97_05695"
     CDS             6601..8139
                     /locus_tag="VHS97_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447724.1"
                     /translation="MKDNRNVSRRGFIESAGAATGAMMAAGAFPHPAVGAVKGANDKV
                     NIAILGPGGRAQEHLRILLKMKNTEHKPIDIIGLCDVWDGAKWKEGARQRETGRGLYY
                     SAEKCGLDWEGKDKDRVTKDYRKILDNKDVDVVLIATPDHWHAKMSIDAMEAGKDVYC
                     EKPMTHTIDEARRVAEATKRTKQVFTVGVQSTADPRWRMANQMITEGKIGHVLQGQTS
                     YYRNSSEGQWRYYPLLKEMTPKTVDWKMFLGTEFGLAPDQPFNRARYAQWRCYWDFGG
                     GMYTDLFVHQLTHLILAMGVRLPRRVVGAGGLYMEYDGRDVPDVATVVADYDEGCQVM
                     ISATMCNDVQLPEVIRGHNATLMFEPKPEEGFRSVPQVFRGGPAPPGTNRGEGGEKFK
                     PEQPGEDTRALWEHYLECVRSRNPETLCPADLGYAAITTVNLGVLSYREGKAYYFDKE
                     TGQPSDADRAWSARWEERSHLRGKPNQVIGWKAGTEGSLLKSPDYQKLEGDWFDGKDP
                     AASA"
     gene            8478..>8914
                     /locus_tag="VHS97_05700"
     CDS             8478..>8914
                     /locus_tag="VHS97_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447725.1"
                     /translation="MTPTAIGRSLILTTVAACLAGCGGDASKPKLGRVSGKVTYNGQP
                     VTKGIVSFVPRGGAGAQTGQSATGELGSDGSYALTTFDNGDGAVLGEHTVLVIAREED
                     AAIKGHGMPIPDSKGQIKIKAAKTLVPEKYATAGNSPLRYTVN"
BASE COUNT         1738 a         2651 c         2772 g         1753 t
ORIGIN      
        1 atcttcgtca tgggccattc cgccggagcg cagctcgccg cgctcgtcac catcgatgat
       61 cgctatttga aggccgaggg ggttccgttc gacgtcctca aaggatgtgt ccccgtcgac
      121 ggcgacacct acgatgtgcc ggcaattatc gagaccggcg aaacgcgccg ccgagtgcat
      181 ggccagccgc agccaaagtt tggtcatcgt gaaaagttcg gcaacgatcc ggaaaagcat
      241 cgcatttact ctgccgtcac tcacgtggcg aaggacaagg gcatccctcc gttcctgatc
      301 ctctacgtcg ccgaacatcc cgacacctcg gcgcaggccc agcgtctcgg caccgtgctg
      361 aaggatgcgg gcattcccgc caaagtcttc ggtgcccaag agaccaacca ctcccggatc
      421 gacgccaacc tcggcacctc cgatgatcca gcgacgaagg cgttgttcga gtttgtgagt
      481 aaggctttga acaactgagg ccattccggc tcttgcgcgc ggcgcggacc gggtcgctgt
      541 gttctccgat cagtcatccc acgcctcgtg gcggctccct tgcgccctct gcaccgtgcg
      601 gttcccgacg tccgcgctct tcgaccgtct cgttgacttt cgactcgcct gacttagaat
      661 gcatgggtca tacctctccg tcggactgga gcgtatgttc ggtaacttct ttctcagcaa
      721 tgacttcgcc catgcgcctc ctgactgcca tccctgtcta caatgaagag gcaagcctgg
      781 tgggcgtgct gaccgaggtc ctcaagtacg cccgcgatat cttggtggta gacgacggtt
      841 catccgatcg gacgcctgcg ctattgcgcg actttccgac ggtgcggaca attcgtcatc
      901 ctcgcaacct gggctatggg gccgggttgc gcacggcttt tcaggcgacg attgacgggg
      961 gttatgacgg cgtcgtgact cttgactgcg acggacagca cgagccggcg cgaattcccg
     1021 aggtggcctc ccatctggtg gaggccgata tcgtctcggg cagtcgctac ttgcgtgtgt
     1081 ttgaccccag tcaagagcct ccagtagagc gccggcgcat caacgtcgag gtcacacggt
     1141 ggctcaacga atgcctgggg atgaatttga cggacgcgtt ttgcggcttc aaggcttatc
     1201 gcacttccgc actgcggctt tttgacatca cggacgaggg ctatgccatg cctctcgagc
     1261 tgtgggtgca agccgttgcc cataggctga agatcatgga agttcccgtt ccactcatct
     1321 acctcgacga atccagagcc ttcggcggcg cgctcgacaa tgcggaatac cggctgaaac
     1381 actaccgccg ggtcttggag aaggcgctcg agcgagccgg cctgctcgcc ccggaggggt
     1441 gtttgggatg acggcgcgcc gaatcaaggc gccctcgatc gacggcggcg ttctggtgga
     1501 tccgaacccc agcgcaatcg ccggacaaat tgccgccaac gccgaccgac tcagcggctg
     1561 ggatcacgat tttcagggaa gacgcgccaa atggttgcgt tccgcggttc gccaggaagt
     1621 cgttgcattg gcccgcagtt ttctcgctcg ccacggcttt tgcgattcga gcctcgagat
     1681 cgaggctttg ggccgaaatg actgcccctt gatcgtgacc gggcatcagc ccgaattgtt
     1741 tcaccctggc gtgtggatca aaaactttgc ggccgcttcg atcgcaaccg ccaacggtgg
     1801 tgtcggcctc aacctgatcg tcgataacga cattcccaag tcgacgtcaa tcgctgtgcc
     1861 ggcagttggt gcccagggga ttcggcttac acgcgtcgag tttgaccgtt ggggtggtga
     1921 tgcccccttc gaagactcgc cggtgctgga agaagagcga ttttccacat tcgccgaccg
     1981 cgttcatgcc gttatgggcg actcgattcc caatcccttg ctcgatcgtt tttggccgtg
     2041 cgcacttggg cgccgcccag aggcttggac ccacggcatg cgtttttcat tggcgcgccg
     2101 tgagatcgaa gagtcttggg gcgtgatgaa cctcgaggtt ccgctgagcg aggtatgtca
     2161 gagcgacggg ttttactggt tcgtctcgca cctgctggcc gagctctcca gatatgcccg
     2221 ggtgcacaac gaggcactca ctgagtatcg cgcgatgcat gggatccgaa gcaagaacca
     2281 tccggtcgcc gcgctcgcta cggaggggga gtggctcgag gctccgttct ggatctggcg
     2341 ggccgagaat ccgcggcgcc gtccactttt ggtccggcag cgcacaaacg ttatggatct
     2401 gcgcatcgcc ggcgaagacg aggtcttgat cgagctgccc ctggcgccgc accgcgaggc
     2461 gtgttgtgcc gtcgagcgtc tccgagagct gccggcacgc tcggtccggc tgcgcacgcg
     2521 tgccttgacg acgacacttt tcagccggtt cctgctcggc gatctgttca tccacggcat
     2581 cggcggagcc aaatacgatg agctcggaga tgagatcgcc cggcggtttt tccaagttga
     2641 gccccccggc ttcctgaccg tctcgatgac gctttggctg ggcttgcccg ccgaacccgc
     2701 gtccgcggcc gacttgatcg acgtcaatcg ccggttgcgc gacgtgcaat tcaaccccga
     2761 cctctgtcta tccgaaccgt acgatgagga actacgtaat ttagttagga agaagcgcga
     2821 agcaatcctt cagtctgtgg cgtctcgacg agagcggaag gtcagacgac tggcaattcg
     2881 ccgcgcgaac gaggcgattc ggccgtgggt gcgccatcag gaggacgaat tcctgggccg
     2941 taagctcgag attttgcggc gtctacggtc gaaccgggcg gcgcggaacc gtgagttttc
     3001 gtcggtgctc catgccgagg accggctgcg gagcagctta cttgaagccg catcgtcgtc
     3061 ggcacggttg atcgagacgt cggtttcgtc gggagcgcct gggcagactt cttgagcgac
     3121 tactccagat tcatttgatg cgcaggctgc cggtcgtagt cgtggttttt gcgcgggcgt
     3181 atagcccatt tttgtgagtg gcagtcacct tatcataaaa aatctggtgg tcgctgtctt
     3241 tttgaggcac gccgccttga gagcccgtac aatgaaaaca aaacgcggct tgatgacacg
     3301 cgttgagctc cccaggacaa gagcagacgg ccgcgttgcg cggcgagtct caaccatggt
     3361 cgacctgatg aaagcgcctg cgccgggaaa tcgacttcct tttcgccgtg gctcggtcga
     3421 gtattgcctc gccaccgaag ccgatcatga agcgatctac cagactctat tgcatgtttt
     3481 tcatggaccg gatcgcgagt cgttcttggg tgcgctgtcc gaccccgtgt atcggcccga
     3541 tcagcgtatc ctcgccaaag tcgatggtcg catcgtcagc catgtccacc tgaccgaacg
     3601 gcaggtccgc tacggctcgg tcacgattcc catcagtggg gtaatgtggg ttggtacctt
     3661 gccggaattc cgcggaatgg ggtttgcaca aaacctgatg cgcctggcca gtctgcgagc
     3721 gcgtgagaac ggtgtggcac tgcaggtcct gacgacgggc atgccacagt tctaccgctc
     3781 gatcggatgg ggtatctgtg gccgtcagtc gtttgctcag accatgagcc gcaatctgcc
     3841 gcaggctact gacggcgtga tcgaggggcg cggcctatca tggcaagtcc ggccctggcg
     3901 gcaggtcgag cttggggacc taatgtcgtt gtacgagcgg caatacgccg agaccaccgg
     3961 cacgatcgtt cgatccgagg aatactggcg ctggatgatc ggccgcaagt atgcgcacgt
     4021 aatctgggtc gcgtgccagg gcgagaccgt gcgaggctac gcgttcatta aagaccacaa
     4081 ggttctcgag atcgccttcg actcgaacta tccccaggcg ctgcgggcgc tgctcggccg
     4141 cgtgcgggcc gaggcgctcg agcgagctta tcccgaagtg accgtgtacg cccccagcga
     4201 tcatcctgta atcgagaccg tgcggaccgc ttccggtcgg atcatcgacc aagatgctta
     4261 cgagggtacg gtatcgatgt atcacattcc cgaccttggg cggtttcttg ggtgcattgc
     4321 gccggaattg gcgcgccggg cgaccgagtc ggggggaacg ctcccgttgg agctggggat
     4381 ctgcctggaa gacgagcgct ggctaatcca cattgatggt aagaactcac gcgtcgagcg
     4441 cgagaagctc agccgacggc acctgacgct cacgccgtcg gccctggtga ggctgctgat
     4501 gggtcatacc ggcatcgcca acgcttcgga cgaagacggt tttatcgcgt cgacgagcac
     4561 ggctctggac gcggcgcgca tcctgtttcc tcaaacggcc atctggcgca gcccgctcga
     4621 tagcgcgacc gcatgaagtg gcacgggctt ccagtccgtg tctgaaaaca ccacgggcaa
     4681 gatgcccgtg ccacttattc ttttgcggtt acttagtata cgtcccgttc gccgcgcgct
     4741 agaaagagat acttcttgac aggcggtgac atcaggtaaa gcgacagcac ggcataaacg
     4801 agtatgatcg taacgccaaa gacgattttt tgagtgtcaa tgcgttcttc aaacggtcgg
     4861 atcccgatgt tggaatcatc cagatcgatt ggtttgaact cacgtgtgat ttggtctgga
     4921 tcgtcgatca tagcgcgtac gattggaaag gcgatcaggc cgatcacgtc gacggcaaca
     4981 cccagcgcga gtgcgagcat cagcagcttg acggacttac ctcggatgaa ctgaacgcat
     5041 gcgtagatgc cgaagcccga gaccagcgcc aggacgagcc aagcgtagtt ttgcatgctt
     5101 gattcgctgc gggtcgactg aatgacgccc aagagcgtga agatgaggcc agccgtcccg
     5161 cagagcccgc cgacgatggc tacgtgcagg ggcggcccgt cagatcgagg cggtggtggt
     5221 ggggcaaagt cgtcctcgag accgacgcgc atgccggtct cttggtcggt accgcacgtc
     5281 gggcagagag acatgccctg gggcacaccc ccgccgcagt gagtgcaccg acgcgcccgg
     5341 gttcgcgctt cggcggccgt gggacgtcgc ttcgaccgac cgtcatcctt gaaaagcgcc
     5401 gcgacgtcgg cagtccccga gcccacgctc gcctcaccgc gctcggcttc ccttcccccg
     5461 ccgctgacga gcggtaaatc aaacgtgtcg cgcaagtcac cttcggatat cgggatcggc
     5521 aagtcttctg gggtgggagg gcgtttatcc atatcaaatg acgttgccgc ggcatcggcc
     5581 tgtcccggaa gccccgataa cgaactggcg tcatcctggg acaccacaaa tcgagtcgcg
     5641 catttgggac acttgaatcg cttgccggcc gcgacgcggt caggcaaatt taagacgacg
     5701 ccgcatcgat tgcattgaac agttcggggc atgggtaatt gatcgcgcgg atcctctcct
     5761 ctgcatgctc cgatggcatc gagccgcccg ctcgatcgcc gcgatccttg gtcccgtgtg
     5821 actcgactca actatcctac ccctcttgat acgtcgagcg tgagtcaaaa gtcgatcgcg
     5881 tcaacgcttt ttcgtccatt cttcatccac agcgcgaggt taagtgcgtg cggtagaccg
     5941 acaagtgttt ttgattcgcg acaaactctg gcgcgttcaa accgctcgac gaaaagcggg
     6001 agcgaactcg ccgccaagcg ccgatcaggc acgaagtgcc ttaccggaag agcggggtcc
     6061 gaggaaacga tagcgcagtc atgccaaaaa ccttcgtgcg ctccggtcta gcatagctca
     6121 tcaaagaatg ttgctcgaga ccgaaacaca aatgtgcccc tgctcgaaga aacccgtgcg
     6181 gcctgtccga cgccggcaaa gtaggcgact gagccttgcc atctcatcgg ttcattttgc
     6241 tggacaagaa cgtcggtgtc aatcaagaga tatgctaatg gcgaacgcgg aacgatttag
     6301 ccgaagagcg gcgataaaga caaggttcgc tcccgactta cgttgcggcg agagaacgtg
     6361 ggccggcatc tgcttgaccc acgactgccg gcggcgttaa cgtgtggact agtatcagcc
     6421 ttgattgata aaactgtggt ttgggcagcg gtaggtgcaa aggctggttt gacaggctag
     6481 agcgtgcggg ccaaacgggc gatcgggctg ggcttggcga gccgcaagcg gtggtgccgc
     6541 cgacccgaaa gcactcgaaa tggccgtggt cttgcttcca gaattccgga gtctattccg
     6601 atgaaagaca atcgcaatgt aagccgacgg ggtttcatcg agtcagcggg tgccgcaacc
     6661 ggcgccatga tggccgccgg ggcgtttcct catcctgccg tgggcgcagt caagggggcc
     6721 aatgacaagg tcaatatcgc gatcctcggc ccgggcggcc gagcccagga acacctacgc
     6781 atcctgctca agatgaaaaa cacggagcat aagccgatcg acatcatcgg cctttgcgac
     6841 gtttgggacg gtgcgaagtg gaaagagggc gcgaggcaga gggagactgg acgcggactc
     6901 tactactccg cggagaagtg tggcctggac tgggaaggca aagacaaaga ccgtgtcacc
     6961 aaggattatc gcaagatcct tgacaacaaa gacgtcgacg tcgtcttgat cgccactccc
     7021 gatcactggc acgccaagat gtcgatcgac gcgatggagg ccggcaaaga cgtctattgc
     7081 gaaaagccga tgacgcacac gatcgacgag gccaggcgag tcgccgaagc gaccaagagg
     7141 accaaacagg tcttcacggt gggcgtgcag tcgaccgccg atccccggtg gcggatggcc
     7201 aaccagatga tcaccgaggg gaagatcggc cacgtcctgc agggccagac aagctattat
     7261 cgcaacagca gcgagggcca gtggcgctac tatcctctcc tcaaggagat gacccccaaa
     7321 acggttgatt ggaagatgtt cctggggacc gaatttggct tggcccccga ccagccgttc
     7381 aatcgagccc gatacgccca gtggcggtgt tactgggact ttggcggtgg aatgtataca
     7441 gacctgtttg ttcatcagct cacccacctt atcctggcga tgggcgtgcg gctgccgcga
     7501 cgggtcgtcg gcgccggtgg gttgtacatg gagtacgatg gtcgcgacgt tcccgacgtg
     7561 gccaccgtcg tcgccgacta cgacgagggc tgccaggtca tgatctcggc caccatgtgc
     7621 aacgacgttc agcttcccga agtgatccgc ggtcacaatg cgaccctgat gttcgagcca
     7681 aaacccgagg aaggttttcg cagcgtgccg caggtgttcc gaggtggacc ggctcctcca
     7741 ggcaccaatc gcggcgaggg gggcgagaag ttcaagccgg agcaaccggg cgaagatacg
     7801 cgcgccctct gggaacacta tctggagtgc gtccgctccc gtaatcccga aacgctctgc
     7861 ccggcagatc tgggctatgc cgcgatcacg acggtgaacc tgggcgttct ctcctaccgc
     7921 gaaggcaagg cctactactt tgacaaggag accggtcagc cgtcagacgc agaccgggcg
     7981 tggtccgctc gctgggaaga acgcagccat cttcgtggca agccgaacca ggtcattggc
     8041 tggaaggccg gtaccgaagg gtcgttgctc aagtcgccgg actaccagaa gctcgagggc
     8101 gactggtttg acggcaagga tccggctgcg tcggcctgag tgtaggccgg acgctaataa
     8161 tcggaaagca taaatcgagg gagggctgca aacgtgcggc tctccctcgc tgcatttcgg
     8221 atttcgcggc ccgagtcggc gggcgcgcca acggattctc gcaagtgcgg gggatctgca
     8281 ccggggatct cggcatgaag gcgtcccagt cctcgttgac ctcctggcgg cgtttcctcc
     8341 gtgcttccgc cgagcctttt ttggcaagag tgttgacgcg accccgccgg cctggttcaa
     8401 acaacactgt gtcgcactcg caccgcacgc gatcttatct ttcgccgtaa cgccgccctc
     8461 cggaaaggca gactctcatg acgcccacgg ctatcggacg cagcttgatt ttgaccacgg
     8521 tggccgcgtg cctggcgggc tgcggtggcg acgcgagtaa gcccaagctt ggccgggtct
     8581 cgggcaaggt gacgtataac ggccagcccg tcaccaaggg gatcgtctcg ttcgtccctc
     8641 gtgggggggc cggcgcccag actggccagt cggccacggg ggagctcggc tcggacggat
     8701 cctacgcact gaccacattc gacaacggcg acggggcggt gctgggcgag catacggtcc
     8761 tcgtgatcgc gcgcgaggag gacgccgcga tcaaggggca cgggatgccg atcccggact
     8821 cgaaaggtca gatcaaaatt aaggccgcga agactctggt gcccgagaag tatgcgacag
     8881 ccgggaattc tcccctgcga tacacggtca atga
//
LOCUS       DATAQL010000335         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4306789, whole genome shotgun sequence.
ACCESSION   DATAQL010000335 DATAQL010000000
VERSION     DATAQL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4306789"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VHS97_05705"
     CDS             <1..306
                     /locus_tag="VHS97_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447726.1"
                     /translation="AQDAKTGSHRLDRCIFLPVEEMTMDPTEEGLTFDEIKERYAPDW
                     VLIGDPQTDEYQRVLGGTVLFHSPNREEVYQKAIELRPGHFAFRFLGTFPEDMVFVL"
     gene            303..692
                     /locus_tag="VHS97_05710"
     CDS             303..692
                     /locus_tag="VHS97_05710"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX3447727.1"
                     /translation="MNHPFNASSGPILVDAEISGPAGKARATLVLDTGATASTINISV
                     LRSAGYDPNLSTDFVQMMTGTGTGVTKVPRVILNRMSALGQYQIGIRVLAHDLPLVAN
                     VDGPLGLDFLRDQVLTIDFRAATMNLV"
     gene            complement(705..2267)
                     /locus_tag="VHS97_05715"
     CDS             complement(705..2267)
                     /locus_tag="VHS97_05715"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX3447728.1"
                     /translation="MNLDLILKGGWVIDGSGGPPFRADVAVLDQMIADVGRLETVEAV
                     RAIDVSGRYVVPGFIDAHVHGDLALLADPIHLPALRQGVTTYLIGQDGSSFAPASAKT
                     LDYMRRYTAGFNGNPPNLTYDWRTVDEYLSQFDRRVAINVAYLIPNGNIRMEVMGLDT
                     RPARDDELRAMQKMVREGMDAGAIGLSTGLDYIPSLYADAREIAALCEPIVAENGVYV
                     THMRGYGPRAGAGMREVFDIARTTGVPAHISHYNGRADLLLSLIDEGRTLGLDLTYDT
                     YPYLAASTILGMVALPGWVQEGGIEPTLERLSDPAVRVRLKSEWFSAPTPYALENLTI
                     AMAAHPEWRWSEGLTVTAAAENAGLDPCDFICDILSASGMAVGIVGFRPGDRTESDVR
                     AILSHTAHMAGSDGIFCGGFPHPRGWGAFARYLGYHTRELGDYSWPEAITHLATHAAR
                     RFRLTDRGLIRPGFVADIVVLDPASVTDRATYASGRALAEGVEHVLVNGTLALENGEP
                     TGTTPGRALRRG"
     gene            complement(2264..2995)
                     /locus_tag="VHS97_05720"
     CDS             complement(2264..2995)
                     /locus_tag="VHS97_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX3447729.1"
                     /translation="MNDAAKKRRTNRQRSVRISNRWAAAVKHLRRVDPHLRSIIDRVG
                     PCLLEPHIDRFSALVRSIIGQQISSRAALSINRRLVEVGGEPHYPARLIELGEANLRT
                     VGLSSAKARYVLNLAEAVVSGQLPLDQFDDSWDDETITQSLTAIKGIGAWTAEMFLIF
                     ALNRPDILPTSDLGVRVGLRDRHGLAELPKPRDCHALAEAWRPYRTIASWYLWKAAGP
                     TIASPAPTAPRISRGARRVRPGQSQ"
BASE COUNT          656 a          959 c          986 g          600 t
ORIGIN      
        1 gcccaagatg ccaaaactgg aagtcatcgg ttagatcgat gcattttcct tccagttgag
       61 gagatgacca tggacccgac ggaagaagga ctgaccttcg atgaaatcaa agagcgttac
      121 gcccccgact gggttctgat cggcgatcct caaaccgacg aatatcaacg agttctcggg
      181 ggcacggtgc tgttccacag cccaaatcga gaagaggtct atcagaaagc tattgagctt
      241 cggcccgggc actttgcttt ccgcttcttg ggaacgtttc ccgaggacat ggtgttcgtg
      301 ttatgaacca cccattcaac gcgagcagcg ggccaatcct ggtggatgcc gagatttcgg
      361 gacccgcagg gaaggctaga gcgacacttg tactggacac cggagcaaca gcgagcacaa
      421 tcaatatctc agttctcagg tcggccggat acgacccaaa cctctcaacc gatttcgtcc
      481 agatgatgac gggaaccgga accggagtga cgaaagttcc cagggttatt ctcaaccgaa
      541 tgagcgcgct gggacaatac cagatcggca ttcgtgtatt agcccacgac ttgccgctcg
      601 tggccaacgt cgacgggccg ctgggactgg atttcttacg tgaccaggta ctgacaattg
      661 acttccgagc cgcaaccatg aacttggttt gaaaccgttt cggttcaccc ccgccgcagc
      721 gcgcgccctg gcgtggtccc cgtcggctct ccgttctcca gagccagcgt cccattgacg
      781 agcacgtgct cgaccccttc ggccaaggct cggcctgagg cgtacgtagc acggtcggtc
      841 acactcgccg ggtcgagaac gacgatatcg gccacgaaac caggacggat caggccacgg
      901 tcggtgagac gaaagcgtcg cgcagcgtgg gtggccaggt gagtgatggc ctcaggccac
      961 gaataatcgc ccagttcgcg cgtgtggtag ccgagatacc gggcaaaagc tccccagccg
     1021 cgcgggtgag gaaagccgcc gcagaaaata ccatctgagc cggccatgtg agccgtatgg
     1081 ctgaggatcg cccgaacatc agactcggta cgatcacccg gccggaatcc cacgattccg
     1141 accgccatcc ccgacgccga gagaatgtcg cagatgaaat cgcagggatc gagcccggcg
     1201 ttttcggccg ccgccgtgac ggtcagtccc tcggaccaac gccactccgg gtgagcggcc
     1261 atggcgatcg taagattctc tagtgcgtac ggggttgggg cagagaacca ttctgacttg
     1321 agcctgacgc ggacggctgg gtcgctgagc cgctcaagag ttggctcgat tcctccctcc
     1381 tggacccagc ctggcaaggc gaccatgccc agaattgtgc tggcggctag atagggatac
     1441 gtgtcgtagg tcaggtcgag cccgagggta cggccttcgt cgatcaacga aagtaagaga
     1501 tccgccctac cgttgtagtg cgagatgtga gcgggaacgc ctgtcgtccg ggcgatatcg
     1561 aaaacctcgc gcatgcccgc acccgcacga ggcccgtagc cccgcatgtg ggtgacgtac
     1621 acgccatttt ccgcgacaat tggctcgcag agcgcggcga tctcacgcgc gtcggcgtac
     1681 aggctaggga tgtaatcgag gccggtagac aggccgatgg cgcctgcgtc cattccctct
     1741 cgtaccattt tttgcatggc gcgcagttcg tcgtcgcggg caggtcgcgt gtctagcccc
     1801 atgacttcca tgcggatgtt gccgttgggg atcaggtacg caacgttgat cgccacccgg
     1861 cggtcgaatt gggacaggta ttcgtcgacg gtccgccaat cataagtgag gtttggggga
     1921 ttgccattga agccggccgt atagcgcctc atgtagtcca acgtcttggc ggacgcaggg
     1981 gcgaacgagc tgccgtcctg accgatcaag tacgttgtca cgccttgccg gagggccggc
     2041 agatgaatgg gatcggccag aagtgcaagg tcgccgtgca cgtgagcatc gatgaagccg
     2101 gggacgacgt atcgcccgga cacgtcgatg gcacgaacgg cttcgaccgt ttcgagtctc
     2161 ccgacgtcgg cgatcatctg atccagcacc gcgacatcgg cacgaaaagg aggtccgccc
     2221 gacccgtcga tcacccagcc acctttcaga atcaggtcaa ggttcattgg gattgacccg
     2281 gcctgactcg cctcgccccg cgactgatcc tgggcgccgt gggcgcggga ctggcgatcg
     2341 tcgggcccgc tgccttccag aggtaccaac tggcgatcgt gcggtacggc cgccaagcct
     2401 cagccagggc atggcagtca cgcggtttag gaagctcagc caagccgtgg cggtcgcgca
     2461 acccgaccct cacgccgaga tcgcttgtcg gcaaaatgtc aggccgattg agcgcgaaga
     2521 taagaaacat ctcggctgtc cacgcgccga ttcccttgat cgcggtaagt gattgggtaa
     2581 tcgtctcgtc atcccatgag tcgtcgaact gatcgagtgg caactgtccg cttacgactg
     2641 cctctgccag gttgagaaca tagcgagcct tcgcagacga aaggccaacc gtacgcagat
     2701 ttgcctcgcc aagctcgatc aagcgtgccg gatagtgcgg ttcgccgccg acctccacga
     2761 gacgccgatt gatcgatagg gcggccctgg atgagatctg ctgaccgatg atcgagcgga
     2821 cgagcgcgct aaagcggtcg atgtggggct cgagcaggca cggaccgacg cggtcgatga
     2881 tggaacgtag gtgagggtcg acccgccgca ggtgtttgac ggcggcggcc cagcgattcg
     2941 aaatcctgac ggaccgttgt ctattcgtcc tgcgtttctt agcggcgtcg ttcatgacga
     3001 caagcgactc gcccctcctt ttgacgcgga ttcttgaccg taaggctgat ggagaacgtg
     3061 ctgcggattc gattccaggt agcgtcgcac ccggtcggcg cgatccagcg cctttggtat
     3121 aaacgccatc gcctgatcga tgcgttcgtc aggctcggcg cagctaaacc gcaagaaccc
     3181 gcggcctgcg tcaccgaagc a
//
LOCUS       DATAQL010000336         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4461107, whole genome shotgun sequence.
ACCESSION   DATAQL010000336 DATAQL010000000
VERSION     DATAQL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4461107"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VHS97_05725"
     CDS             <1..571
                     /locus_tag="VHS97_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3447730.1"
                     /translation="HAGGYAPPRVRIAQGVGFSSEDRKTEGLALNRSIEDNLTYSALR
                     RHARWGWLRLRERRVEVSHWIARLRISASGPRQSIASLSGGNQQKVALARLLHQRADV
                     LLLDEPTRGIDVGSKAEIYRLIGEQAALGKAILVVSSYLPELFGICDRIAVMSRGALC
                     EARPVAEWTEHEVMDVATRADKGQFQVIE"
     gene            638..1699
                     /locus_tag="VHS97_05730"
     CDS             638..1699
                     /locus_tag="VHS97_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3447731.1"
                     /translation="MAESAVDRRQAPPGIEMRGTLSWVWTLLGPFLGLVLIVALFGTI
                     TRDSGTFLTADNWRTIAVQTVIVGTAALGMTIIMIAGGIDLSAGSTVALVTVCIAMFV
                     KKVDPLLSDSLRDLKLVLPLALLLGIAIGGLCGMINGALIAGLRVVPFIVTLGSYTVY
                     RGFATWLASSTTVYVPGDVKPWWFTQIMKIEPEPRWLLVAPAVWVLLGLSMLVALALH
                     YSLLGRYMYAIGSNEATARLCGINVPFIKVIVYSLGGLAMGLAGVLQFAYLGAIGDPT
                     TAGGLELQVIAAVVIGGGSLSGGEGTVMGTLIGCLIMQVLKNGCVHAGIPNPSQDIII
                     GIIIVAAVTLDRLRRTKVS"
     gene            2147..>3196
                     /locus_tag="VHS97_05735"
     CDS             2147..>3196
                     /locus_tag="VHS97_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX3447732.1"
                     /translation="MASTSLHARPRQTLGGVKAATRPTTRPCAAARIDRPARWRIASN
                     FAFLSAAELICRMTSVVVTLSLAKRLGVDGYGRIEFAFSVVFLLVLLLRDSSDVIVAR
                     ELSRHPRLIRPLVDQVLAYKTIFALILFSLLALVGSLTLAPSDLRILALYGTMLFTTA
                     VGLDFVFRGTERMGLVAVSLCVRTGIYAFGVLTLVRDSSRIAWVPIWLTAGELSGIAL
                     VWLSYLKYYRLPRPRLSPRFLFILVQRGRTVCLIQLSQAVINSADLLVVGFLSVASDV
                     GRYGAPHRMVTAFLTFGLILQQAAFPTLSRLWRQTAGAGQEALDSLVEVLMTGLVPVA
                     VGCTVLADPLVHLFLP"
BASE COUNT          561 a          923 c         1012 g          700 t
ORIGIN      
        1 ccacgccggc ggttatgccc cgcctcgggt tcgcattgca caaggggtcg gattctcgag
       61 cgaagaccgc aagaccgagg ggctggcact gaaccgctcg atcgaggaca acctgaccta
      121 ctcagcgctc cggcggcatg cgcgctgggg ctggctcagg ctccgggaac ggcgcgttga
      181 ggtgtcgcac tggatcgcgc gattacggat cagcgcttcg ggcccccgcc agagcattgc
      241 gagcctctcg gggggcaacc agcaaaaagt cgcgctcgca cggttgctcc atcaacgggc
      301 cgacgtgctg ctcttggacg aaccgacccg cggcatcgac gtcggatcca aggccgagat
      361 ctatcgcttg atcggcgaac aggccgcgct tggaaaggcc atcctggtcg taagctctta
      421 tttgcccgag ctatttggga tctgcgaccg tatcgccgtc atgtcgcgcg gagcgctctg
      481 cgaggcccgt cctgttgccg agtggaccga gcacgaagtc atggacgtcg cgacccgagc
      541 cgataaaggc cagttccagg tcatcgagta gaattgttcg cagcttcggc tgatccagcg
      601 atcacgactt catatcttaa cagagagggc aacccctgtg gccgaaagcg ctgtggaccg
      661 tcggcaggca ccgccgggta tcgaaatgcg cggcaccctg agctgggtct ggactttact
      721 cggacccttt cttgggctgg tcttgatcgt ggcgctgttc gggacaatca cccgtgattc
      781 aggcacgttt ctcaccgccg acaactggcg gacgatcgcc gtccagacgg tgatcgtcgg
      841 caccgcggct ctgggcatga cgatcatcat gattgccggt ggaattgatc tttcggccgg
      901 ctcgacggta gcgctcgtca ccgtttgcat cgcgatgttc gtcaagaaag ttgatccgct
      961 cttgtcggat tcgctacggg acctgaagct cgttctgccg ttagccctgt tgctgggaat
     1021 tgcaatcggc ggattgtgcg gaatgatcaa cggcgcattg atcgcgggcc tgcgcgtggt
     1081 cccgttcatc gtcacactgg gtagttacac cgtctatcga gggttcgcca cgtggctggc
     1141 gtcgagcacg actgtttatg tgccaggcga cgtaaagcca tggtggttca cacagatcat
     1201 gaagattgag ccggagccgc gctggctctt ggtcgcgccg gcggtctggg tccttctcgg
     1261 actgagcatg ctggtggcgc tcgcgctcca ctacagcctg ctgggccgct acatgtacgc
     1321 catcggttcg aatgaggcga ccgcgcggct gtgcggaatc aacgttccat tcatcaaagt
     1381 gattgtctac tcgctgggcg gactggcgat gggcttggct ggcgttctgc aatttgcata
     1441 cttgggtgcg atcggcgacc cgacaacggc cggcggactc gagctccagg tcatcgccgc
     1501 ggtcgtgatc ggcggcggaa gcttgagcgg cggtgaaggg acggtcatgg gtactttgat
     1561 cggctgcctg atcatgcagg tgctcaaaaa cggctgtgtc catgcgggta ttcccaaccc
     1621 aagccaagac atcattatcg gcatcatcat cgtcgctgcc gtgaccctcg atcggctacg
     1681 tcgcacgaag gtttcataat tgggatgagc cgctgcgccg gtgttggtcg cgcgaggcga
     1741 ccaagcgggc cctttcgacc ttggctgcct acgcgcccac ggcaccgaca taatccaaaa
     1801 cacggctcgt ggggtcattg gttaccattg cacaccctct caagctacgg ttgacagtgc
     1861 agaagcagcc tactaggatg cgcgggcgtt tcatgaatgc tgggccgcga cattacccct
     1921 agcactttca agcaaagagc gaaagccacc aaaagttggc gctgccggaa gtgcaatccg
     1981 cagtagtggt taatgcgaat ctgcccaacg cacaccctca gacggtcgat tcccattaag
     2041 ggacacaaga caggaagtcc gattaatctg ggaagactgt cgactagccg ataaggcttg
     2101 ttatgttgca aatttgggca aactgcacaa ggaagtgctc ttgcgaatgg ccagcacgtc
     2161 cctccacgca cgaccacgtc agaccctggg tggggtaaaa gccgcgactc gtccgacgac
     2221 gcgcccgtgt gccgcggctc gaatcgatcg cccggcgcga tggcggatcg cgtcgaattt
     2281 tgcattcctg agcgcggccg agctgatctg ccggatgact tccgtggtgg tgaccttgtc
     2341 gctggcgaag cgcctgggcg tggatggata cggccgtatc gagtttgcgt tcagtgtcgt
     2401 cttcttgctc gtgttgttgc tgcgtgacag tagtgacgtc attgtggcgc gcgagttgtc
     2461 gcggcatccc cggctgatcc ggccgctggt tgaccaggtc ctcgcgtaca agacgatctt
     2521 cgcgctaatt ctgttttccc tcttagcgct ggtagggtcg ttgacgctgg caccgtcgga
     2581 cttgagaatc ctggccctat acggcacgat gcttttcacg accgcggtcg gcctggattt
     2641 cgtttttcgg ggaacggagc gcatggggct ggtggccgtg tcgctgtgcg tccgcaccgg
     2701 catatatgcg ttcggagttc tgactttggt ccgggactcc agccggatag cgtgggtgcc
     2761 gatctggctg acggcgggcg agctaagtgg gatcgcgctg gtctggctca gttatttgaa
     2821 atattatcga ctgccccgac cgcggttgag cccgagattc ttattcatct tggtgcagcg
     2881 tggacgcacg gtctgcttga ttcagctctc acaggcggtg atcaattcgg cggacctgct
     2941 cgttgtcggg ttcctgagtg ttgcaagcga cgtcggccgg tacggggcac ctcaccggat
     3001 ggtgacggca ttcctgacgt tcggtctaat cctccagcaa gcggcgtttc ccactctctc
     3061 tcgattgtgg cgccagacgg cgggtgccgg gcaagaagcg ctcgactcgc tggtcgaagt
     3121 gctgatgacc gggttagtgc ctgtcgccgt gggctgtacg gtgctggccg atccgcttgt
     3181 gcacctgttt ctgccg
//
LOCUS       DATAQL010000337         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4155122, whole genome shotgun sequence.
ACCESSION   DATAQL010000337 DATAQL010000000
VERSION     DATAQL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4155122"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VHS97_05740"
     CDS             <1..345
                     /locus_tag="VHS97_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447733.1"
                     /translation="IKLHRYSGKVSYLVYPDFETNPHPALRRSVKLSLRTREIDCFEY
                     TASSNPPILHRKETFLTADQRLQAKFARLTQQEEKHGLLDDTATIGTKVGWQARLDAS
                     GFALRGRRLVRR"
     gene            913..1278
                     /locus_tag="VHS97_05745"
     CDS             913..1278
                     /locus_tag="VHS97_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447734.1"
                     /translation="MSTPVVTVEGVVRSDGSVEVSEKVSLPAGRVQVTLVPWPQLPQD
                     DPFWQMMQKIWDGQKARGHVARTSSEVEAEREALREEWDTRMSEITRIQEEADKLRAG
                     RQPELRCTSMRIVSSISSN"
     gene            1236..1637
                     /locus_tag="VHS97_05750"
     CDS             1236..1637
                     /locus_tag="VHS97_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX3447735.1"
                     /translation="MYLDANCVIYLVELNLIWGPKVLARVSTLRSAGNAVAVGDLART
                     ECLAHPFLKGNAAVVADFQAFFSSPDVDVLPMTAGVCERAARIRAASLFQLKVPDCLH
                     LATAIEHGCDLFLTNDVALRRCTQITVEILS"
     gene            1739..1909
                     /locus_tag="VHS97_05755"
     CDS             1739..1909
                     /locus_tag="VHS97_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447736.1"
                     /translation="MLKADHPIHAKIAPLTQQEEKHGLLDDTATIGTREGWQSRLAAT
                     GSALRGHRLVRR"
     gene            complement(2078..2287)
                     /locus_tag="VHS97_05760"
     CDS             complement(2078..2287)
                     /locus_tag="VHS97_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447737.1"
                     /translation="MAFPFASKVDGTFPRVLAKVPDAAGRHRLTDEVGVGVFAALTLA
                     NGRFYYRRFKLRGRCMRLMENSDDL"
BASE COUNT          533 a          733 c          729 g          471 t
ORIGIN      
        1 atcaagctgc atcgttattc cggcaaggtt tcttaccttg tttatcccga tttcgagacc
       61 aacccgcacc cggcgctccg ccgcagcgtc aagctctcgc tccgcacccg cgagatcgac
      121 tgcttcgagt acactgccag ctccaacccg ccgatcctgc atcggaagga gacgttcttg
      181 acggccgatc aacggctcca agccaaattc gcccgactga cccagcaaga ggaaaagcac
      241 ggtctgctcg acgacacggc gacgatcggg acgaaagtcg gctggcaagc caggctggac
      301 gcgtctggct ttgcgctccg cggccgtcga cttgtgcggc gatgattcac tggcaccacg
      361 cggagctatc cgaaaacccc gaacgcttaa ccacggacag ctcggaccgg cacggaaagc
      421 gacgtccgat agcgaccatg cagcgcagga gagaacggag tgcctgatgc catcgtgcga
      481 cgacgaagat gaacgattga tcgccagaag ccaggttttg gcgagagccg ccaattcttc
      541 agcgacataa gctaccagac gcgaaccact cgccgattga agatatgtct tgacggacgc
      601 cgttgacgaa caggcgacaa ccgctaaatc acagcggcga tctgtcaacg ccccgcgtgc
      661 ccgttccgat cctttcgccg tttcatgttt gctgctcgcg tcggtggggt gtgatcgaca
      721 tctgagacca gccacagcat ttcaaggact cccgcgctaa gcaaggaagc cgtctggcgt
      781 gtaaggacaa gaaatcattg caaggaaatc gaccgcgatc ctgggtaaga tgaattcatc
      841 cctgttcgga agcggcaagc gctctgcatg gccgtgggga cgcttgctgc ggcgagaatg
      901 aaaggttaga cgatgagcac acccgtcgtg accgtggaag gggtcgtgag gtcggatggt
      961 tcggtcgagg tgtcggaaaa ggtgagcctg ccggcgggca gggtccaggt gacacttgtg
     1021 ccgtggccgc agctcccgca agacgacccg ttctggcaga tgatgcagaa gatctgggac
     1081 gggcagaagg cgcgcgggca tgtcgcccga acctcctcgg aggttgaggc ggagcgcgaa
     1141 gcgctgcgcg aagaatggga cactcgaatg agtgagatca ctcgcatcca ggaggaggcc
     1201 gacaaactca gggctgggag acagccagaa ttacggtgta cctcgatgcg aattgtgtca
     1261 tctatctcgt cgaactaaat ctgatttggg gacccaaggt tctcgcccgc gtttccacgt
     1321 tgcgtagcgc cggaaacgcg gtcgctgttg gtgacctggc tcgtacagaa tgcctggcac
     1381 atccgtttct caagggcaat gccgcggtcg tcgctgactt ccaagccttt ttcagtagtc
     1441 ccgacgttga cgttttgcca atgaccgcgg gggtatgcga gagagccgcc cggatccggg
     1501 ccgcgtcact ttttcagctg aaggttcctg attgcctcca tctcgccact gccatcgaac
     1561 acggatgtga cctgttcctg accaacgatg tcgcgctgcg aaggtgcact cagatcactg
     1621 tagagattct gtcgtaacct cgtttatccc gatttcgaga ccaaaccgca ctcgcgagat
     1681 cgactgcttc gattacaccg tccgtacgaa tccgccgatc ctccatcgca aggagacatt
     1741 gctcaaggcc gatcatccca tccacgccaa gatcgccccg ttgacccagc aagaggaaaa
     1801 gcacggtttg ctcgacgaca cggcgacgat cgggacgcgg gaaggctggc agtccaggct
     1861 cgctgcgacg ggctctgcgc tccgcggcca tcgactcgtg cggcgatgat tcactggcgc
     1921 ctcgccgagc cacccgaaaa ccgcgaaggt ctatccgagg ctcgctggcg aattgaccgt
     1981 tcccgaaatc gtcggccggc tcgccgctat tggcgcggaa cggtatcatg tcgactacag
     2041 ccggcagcaa atcacctact atctggcgaa tgaagactca caggtcgtcg ctgttctcca
     2101 tgagccgcat gcaacggcca cgcagtttga atcgccggta gtagaagcgg ccgttcgcca
     2161 gagtcaacgc ggcgaacaca cctacgccga cttcgtccgt aagacgatgg cggccggctg
     2221 cgtcgggtac tttggccaga actctgggaa acgtgccatc tactttggac gcaaacggga
     2281 aagccatgta gagcatttgc tgtccgcgcc ggtggcccaa aaaaaaagtg aggttcgacg
     2341 tgccaagtgt cctcgggatt ctggagacag cgctttatgt caaggatgtg caacgggcag
     2401 ctgctttcta tcggcgacta ttcggtttca cgacgctcct cgattccgaa cggctgatcg
     2461 cgctcg
//
LOCUS       DATAQL010000338         5901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3545709, whole genome shotgun sequence.
ACCESSION   DATAQL010000338 DATAQL010000000
VERSION     DATAQL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3545709"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1753
                     /locus_tag="VHS97_05765"
     CDS             86..1753
                     /locus_tag="VHS97_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447738.1"
                     /translation="MSLFQEERSASARSRRSAAMVRTAAVASSDPGSDRARTLFDVIV
                     GTVIFLGLLLLFLLKAPGIEFYLNSRDHGYQLSIGTQVLLGKVPGIDLPIAYGPLVMY
                     TSALGLWFTHSLIGETILCSTGYALSVFLLYHLVSRYASKWLGLVAAGFGLLLQARFY
                     KWYLWLIPMAILWMSHRYLNSPPTRRWRWIITGGSILGIGWLFRPDFGTTNLLACLVF
                     LGLVEASEPPRGAVRVLRALGLFLASFSIFPLAWLGYVVARCGILAPLTYLGNTVQAA
                     LSIASAMSQPPPPIRSLIVAYWLMPLSYLIAFAIVWQRSRTRKLDVRYWFLLSSALVG
                     TACLHQAMHRMGPAHLLQIIPAAIICTSLIASALFSGAEGLGLPVHAKTLIRVAGVGY
                     AVLLVSIGLKLSRWGQSDLDAFSLWPVDRYSGLADPLGRSDRNPRLAALSTVTKLTNP
                     GEPILVFPIDCQYYALTQRRISGRQVAYFAGLFHSPRDRERNLEAIRAELPKLVIVPS
                     DFETSPETTPEPLVGEGRRAHQNIEGFIRQEYPRVVVNEGGIVVLSR"
     gene            2230..3687
                     /locus_tag="VHS97_05770"
     CDS             2230..3687
                     /locus_tag="VHS97_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447739.1"
                     /translation="MEDTQRTLVILLTMHRSGSSVATSILNRLGMSLGSFKLLGATPS
                     NPHGHFEAIPILELNMDVQKLVYGFAQDLPESEEALDGFVASQAATDWPEIPAHFLAK
                     GRSIIENLLDSGKVSGFKDPRTVLLWPFWERVLTDFPTVRVAPVALLRSPHEIAMSLC
                     SRSLVSGGVYAYWDCLDMTAIHFRRLKKILDDWREPTPFVRFGSSSFLDDLAETARHC
                     GLVWDAEKATEMLDAACIHYLAARVPHEAQHVYDSLCGQCALIDHQANQDRLNADSRV
                     CERMAWRRVRRAESTAKSTLDLLAASQAQLTQSQVEMRHAQDELHRAHEHLHHAPDQV
                     RAAHDLVRDTGKQLREAHDQVRDINNQLLAAHQQVRETNYQLREAYDQLREAYDQLGE
                     AHDQARNLSQNLRETYEHLHRSQEHNTNLVEVIRQANLALEQSHLDAHNLRHICDEQR
                     QNLGKFEEHPLLGAALRGRRRLLRALDTMGLRSAG"
     gene            complement(3709..4848)
                     /gene="wecB"
                     /locus_tag="VHS97_05775"
     CDS             complement(3709..4848)
                     /gene="wecB"
                     /locus_tag="VHS97_05775"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX3447740.1"
                     /translation="MEHVARKTIVCVVGTRPEAIKMAPVIQALHEAPWARCRVLLTGQ
                     HREMLDQALMFFGIEPHVDLDVMRLKLPLDRLAAYLVKTLSSTLASEQPDMVLAQGDT
                     TTVVATAIAAFLQNLPFGHVEAGLRTQCLFAPFPEEANRVVASHLSALHFAPTISART
                     NLEREGISPRTIFVTGNTVIDALALTAERDLPVGIDLDPAKRLILVTAHRRESFGEPM
                     RQICRAVAELHERYSDIEFLWPVHPNPAIEPIVEAMLSRRPRVRLVPPLDYGPFVSAL
                     KRSTLVLTDSGGIQEEAPALGKPVLVLRAESERPEAIASGVARLVGHDPGAIVAEVSR
                     LLDDSAAYGAMAKGTSPYGDGQAARRIVSGVASFLRVADTMRIAG"
     gene            complement(4960..>5901)
                     /locus_tag="VHS97_05780"
     CDS             complement(4960..>5901)
                     /locus_tag="VHS97_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX3447741.1"
                     /translation="QGRGISVVGSSVNVQNIKTDGTLGDGVVVTSYQGHNASLTATSS
                     QFDAVQTGDGLDLRTGSSSLINGCTFNDNGTSPNVTISSVGLALSSNASATILNSQFI
                     GNTNAGLVALQNSAVTAQHSIFADNKKGDGALFLNQTNVNLVDNTFASNGEISGEATG
                     LNGVEFFINFTGSAIVSGNVFENNTAAGIWGGSSSNNIQITNNTFNNNVVGVFLDASA
                     APISATVQGNTFVVPVGSRDPQLVGFLTIGRVTAIVGGDGTLANTIENYADGRYIAES
                     NGGPNAFVGNPNVTILTNTFLSNGMTVPPSQAVTTAG"
BASE COUNT         1177 a         1739 c         1727 g         1258 t
ORIGIN      
        1 cccatagaat cccccgcctt tgtgggctcc catcgtaaag atctttaagt gtaaagatct
       61 ttaagacctg cgccggaggg acgaaatgtc gctatttcag gaagaacggt ctgccagtgc
      121 ccgatcgagg cgcagtgcgg ccatggttcg cacggccgct gtcgcatcga gtgaccccgg
      181 ttccgatcgc gctcgaactc tcttcgacgt gatcgtcggc acggtcattt tcctgggcct
      241 cttgctcttg tttctgctta aggccccggg gatcgaattc tacctgaaca gtcgagacca
      301 cggctatcag ctcagcattg ggacacaggt tctactgggc aaggtccccg gtatcgacct
      361 gccgattgcc tatggccccc tggtgatgta cacaagcgcc ttggggctgt ggttcactca
      421 ttccctgatc ggggaaacga ttctctgctc gacgggatat gccctttcgg tgttcctgct
      481 ctatcacctc gtgtctcgtt acgcgtcaaa gtggctggga ctcgtggccg ccggattcgg
      541 attgctgctg caggcgcggt tctacaagtg gtacctctgg ctgatcccca tggccatcct
      601 ctggatgtcg caccgctacc tgaactctcc ccccacgcgg cggtggcgct ggattattac
      661 cggcggttcc atcctgggga ttggctggct gttccggccc gacttcggga cgaccaattt
      721 gttggcgtgt ctggtcttct tgggtttggt cgaggccagc gagccgccgc gcggggctgt
      781 ccgcgtcttg cgggccctcg gactgttcct cgcgagcttc tcgatctttc cactcgcatg
      841 gctgggctac gtggtggcgc gttgcggcat tctcgctccc ttgacctatc tcgggaacac
      901 ggtccaggca gcgctgtcga ttgccagtgc gatgtcgcag ccaccgcccc cgatccggag
      961 tttgatcgtc gcgtattggt tgatgccgct ctcgtattta atagcgttcg cgatagtctg
     1021 gcaacgttca aggacccgca aactcgatgt gcggtactgg ttcttgctct catcagcctt
     1081 ggtcgggacg gcttgcctac atcaggccat gcaccgaatg ggcccggcac atctcctcca
     1141 gataatcccg gcagcgatca tatgtacttc attgattgct tccgcgctct tcagtggggc
     1201 cgaaggtctg ggtctccctg tacacgcaaa gacgttgatt cgcgtggcgg gggtgggcta
     1261 cgcggtgttg ctggtttcta tcggcctgaa gttgtcacgc tggggacaat ccgatctcga
     1321 cgcattctca ctctggccgg tcgataggta cagcggcctg gccgatcctc tgggtcggtc
     1381 cgaccgcaat ccacgactgg cggcattgtc gaccgtcacg aagttgacga atcccggcga
     1441 gccgattctc gtttttccga tcgattgcca gtattacgca ctcacgcagc gacggatcag
     1501 tggccggcaa gttgcctact tcgccggtct cttccactcg ccacgcgatc gcgagcgcaa
     1561 cctcgaggcg atccgggcgg aattgcctaa acttgtcatc gtgccatctg attttgaaac
     1621 gagccccgaa accacccctg aaccgttggt cggagaaggc aggcgagctc accagaatat
     1681 cgagggtttc atccggcaag agtacccgcg agttgtggtg aacgaagggg ggatcgtggt
     1741 gctgagtcgg taggtggaac aaaaccacaa gcaacggctc ccaaggagcg ctggccgaag
     1801 gctcgaacgc gggccgtgac gttgtggcca cacttcgaca tcgaacagtg agaaccgctt
     1861 ccacatcact agatcgcctg tcccggaggc tctctggaag gtgggaacca aagcacttaa
     1921 atcaggcgga ggaaacaggt aatgaccgta attcaagtaa agttttgtaa aggatttcca
     1981 actccggtta cacgttgccg ccgatgacga tagagtatgc tgtagatgtt atggaattgc
     2041 ggttctttca tgcgcgcgtc tagcaggcgc gagtcggacg tgcatgtcca gctggtcgcc
     2101 gggacagttt cgtttgagac gcgcccgagt ctgcgcctga accagtcttt actggttttg
     2161 gcgagcgcgt agactggaaa aaacgattcg agcagataga cgaccgggtt caatgaaagt
     2221 gagccgacca tggaggatac ccagcgcacg ctcgtcatac tgctaaccat gcatcgtagc
     2281 ggttccagtg ttgcgacgag catcttgaat cgactcggca tgtctttagg ttcttttaaa
     2341 ctactgggag caacgcccag caatccgcac ggacacttcg aggccattcc cattctcgag
     2401 ctgaatatgg acgtacagaa gctcgtgtac ggcttcgcgc aagacctacc cgagtcggaa
     2461 gaagcccttg atggtttcgt ggccagccaa gcagccaccg actggccgga aatccccgcg
     2521 catttcctcg ccaagggtcg atccatcatc gagaacctac tcgactcggg aaaagtctcg
     2581 ggattcaaag atccgcgcac agtgctcctg tggcctttct gggagcgggt gctgaccgat
     2641 tttcctacgg ttcgcgtcgc gccggtcgcg ctcttgcgct caccgcacga aatcgccatg
     2701 agtctttgct cgcggagcct cgtaagtggc ggcgtctatg cctactggga ttgcctcgac
     2761 atgaccgcca tccactttag acggctcaag aagatcttgg acgactggcg tgaaccgacg
     2821 ccgttcgtca gatttggcag ctcgtcattc ctggacgatc ttgccgagac agcgcgacat
     2881 tgtggcctgg tttgggacgc ggaaaaagca accgagatgc tagatgctgc ttgcattcat
     2941 tacctcgccg cccgtgtgcc ccatgaggct caacacgtat acgactccct gtgtggccag
     3001 tgcgcgctca ttgaccatca ggccaatcag gaccgactca acgcggattc acgggtgtgt
     3061 gaacggatgg cctggcgacg agtccgccgc gccgagagca cggcaaaatc gactctcgat
     3121 ctactcgcag cgtctcaggc tcagttgacc caatcacagg tcgaaatgcg ccacgctcaa
     3181 gacgagctgc accgcgctca tgagcacttg caccatgctc ctgaccaggt gcgagccgct
     3241 catgacctcg tgcgcgatac tggcaagcag ttgcgcgagg ctcatgacca ggtgcgcgac
     3301 attaacaacc agttgctggc tgcacatcaa caggtgcgcg agactaacta ccagttgcgc
     3361 gaggcctacg accagttgcg cgaggcctac gaccagttgg gcgaagctca tgaccaggcg
     3421 cggaatctga gtcagaatct gcgcgagact tatgagcact tacaccgctc gcaagaacac
     3481 aacacaaact tggtggaggt catccgtcag gccaacctgg cgctcgaaca atcgcatctc
     3541 gatgcgcaca atctgcggca tatttgcgac gagcagcggc agaacctcgg gaaattcgag
     3601 gaacatcccc tcctgggcgc tgctctgaga ggacgccggc gcctgctaag ggcactcgac
     3661 acgatgggat tgcggtccgc gggctgagca tagtcggctc tctttcggtc agccggcgat
     3721 cctcatcgtg tcagcgacgc gcagaaaact cgcaacgccc gatacgatcc tccgcgcggc
     3781 ctgcccatct ccatacggac tcgtaccctt ggccatcgcc ccgtaggccg cggaatcgtc
     3841 gagcagacgg ctcacctcag caacgattgc gccgggatcg tgaccgacca gcctggcgac
     3901 tcccgacgcg atcgcttcgg gcctctcgct ctcggctcgg agcaccaaaa ccggtttgcc
     3961 gagtgcgggg gcttcttcct ggatgccgcc cgagtccgtc agtacgagcg tagaccgctt
     4021 gagggccgag acaaaaggac cgtagtccaa cggcggcaca aggcgaacgc gcggccgtcg
     4081 cgataacatc gcctcgacaa tcggttcgat cgccggattg ggatggaccg gccagagaaa
     4141 ctcgatgtcg gagtagcgct cgtgcaattc ggcgacggca cggcagatct ggcgcatcgg
     4201 ctcgccgaag ctttcgcggc gatgtgccgt caccaggatc agccgcttgg cggggtcgag
     4261 atcgatgccg acgggaagat cgcgttcggc cgtcagcgca agtgcatcga tcaccgtgtt
     4321 acccgtgacg aagatcgtgc gcgggctgat tccttctcgc tccaggtttg tacgggccga
     4381 tatcgtagga gcgaaatgca gagcgcttaa gtgcgaagcg actacgcggt tagcttcctc
     4441 gggaaatggt gcgaaaagac attgggtgcg aagtccggct tcgacgtgtc cgaacggcag
     4501 attctggaga aaagccgcga tcgccgtggc gacgacggtc gtcgtgtcac cctgggccag
     4561 aaccatgtcc ggttgctcgg acgcaagagt ggaggaaagc gtctttacga gatacgcggc
     4621 cagccgatcg aggggaagct tcaaccgcat gacatcaagg tcgacgtgcg gttcgatccc
     4681 gaaaaacatc aacgcttgat cgagcatctc gcgatgctgg cccgtaagca agacgcggca
     4741 tcgcgcccag ggcgcctcgt gaagagcctg gatcaccggt gccattttga tggcctcagg
     4801 acgagtgcca acaacgcaca cgatcgtctt ccgcgccacg tgctccatcg agccgactcc
     4861 attcgactaa gagtggaatt ttccccctta cgtctccgcg ccgtgcgtgc tccgtcaggg
     4921 ggttccattt cttgaagcag cctacttcct actgcccctc tagccagccg tcgtgactgc
     4981 ctgggaaggg gggacggtca tcccgttgga caggaacgtg tttgtcaaaa tcgtgacatt
     5041 cgggttgccc acgaacgcgt tggggccgcc gtttgactcg gcgatgtaac ggccatcggc
     5101 atagttttct atcgtattag cgagcgtgcc gtcgccacca acaatggcgg ttaccctgcc
     5161 aatcgtcaaa aagcctacta actgcggatc ccgtgagccg acgggcacca caaatgtatt
     5221 gccttggaca gtcgcgctga tcggcgcggc gctggcgtcc agaaagactc cgacgacatt
     5281 gttgttgaac gtattgttcg taatctgaat attattggat gagctgccac cccagattcc
     5341 ggctgcggtg ttattctcga aaacatttcc cgatactatg gcgctgccag taaagttgat
     5401 aaaaaactct acgccgttga gcccggtggc ctcgccgctg atctcaccgt tggacgcaaa
     5461 tgtgttgtca accaggttca cgttcgtctg gttgagaaag agcgctccgt cacccttttt
     5521 attgtcggcg aaaatgctgt gctgggccgt aacggcggag ttctgcaacg cgaccaaccc
     5581 cgcattcgta ttgccgatga actgcgagtt gagaatagtg gccgaggcgt tactactaag
     5641 ggcgagtccg acgctcgata tagtaacgtt gggagaagtg ccgttgtcgt tgaatgtgca
     5701 cccattgatc agcgacgacg aacctgttcg aaggtcgagg ccgtcgccgg tttgcaccgc
     5761 atcgaactgg ctggaagtgg cggtcaggct agcgttgtgg ccctggtaac tcgtcacgac
     5821 gaccccgtcg cccagggtac cgtcggtctt gatgttctgg acattcaccg acgagccaac
     5881 gaccgaaatt ccacggccct g
//
LOCUS       DATAQL010000339         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5224456, whole genome shotgun sequence.
ACCESSION   DATAQL010000339 DATAQL010000000
VERSION     DATAQL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5224456"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VHS97_05785"
     CDS             <1..372
                     /locus_tag="VHS97_05785"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3447742.1"
                     /translation="RMISSQTPTLLLKDGRARVITGSPGGRTIPNTTLWIVLNLVEIG
                     LGPNEAVQAPRTHHQWFPDVLTLEGRSWADPTRMALTAMGHKLRTIEHQGNANTIVVD
                     PEGRVHGIADPRRSTTKASGD"
     gene            1092..1457
                     /locus_tag="VHS97_05790"
     CDS             1092..1457
                     /locus_tag="VHS97_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX3447743.1"
                     /translation="MAKKAAPGTTAPKAAPATGSSSKPATKGEIYTKLATKTGLSKKQ
                     VSTVFDELTELIGNELGKKGPGLFVVPGLLKLKIVRKPATKAKTGINPFTKEPMTYKA
                     KPARNVVKALPLKSLKEMV"
     gene            complement(1609..2571)
                     /locus_tag="VHS97_05795"
     CDS             complement(1609..2571)
                     /locus_tag="VHS97_05795"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3447744.1"
                     /translation="MKIIVTGGAGFIGSHLVDRLLAEGNEVIVVDNFDPFYERATKEA
                     NLNNALGSPRCRLAELDIRDAAGVDTLVAETRPEVIVHLAARAGVRPSIDDPRLYTDV
                     NVVGTVVWLEAASRLEPRPRFVYASSSSVYGDRAEGPFRETDTVDLPVSPYAATKKAC
                     ELLAHTFHHIHGLPVSGLRLFTVFGPRNRPDLAIAKFTRLIDQNQPVPMFGDGTTRRD
                     YTYVDDIVDGICRAIERCTGHHLYNLGHSEPIELKQMIDTIALALGKPAVIQALPEQP
                     GDVKQTFAAIDRARSELGYAPSTPFGEGIRRYVQWYRSRPVLPR"
     gene            complement(2575..>4029)
                     /locus_tag="VHS97_05800"
     CDS             complement(2575..>4029)
                     /locus_tag="VHS97_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447745.1"
                     /translation="LDHLIAALKSRGIFVALELQGKRRFRTGDGVTATGMLPPGGGPA
                     ALFDPTLSKLAIDSARALLGHVNPETGLALKDDPVLAWVTLTGEVTLFNLIDNPDALP
                     AAYAKALQSLAEKAKGGPGRRFWESVEAAHSKRMAEALRKDGLRVPIAGVSHWRREPE
                     FGAAQAGPGLDLIDDRLFWNPALWVSPEKRSLLWCGPDTSLAAQAAMKRRPDRPYVIG
                     QTCNVTQGAWSFPHEAADQFLGVYSALAGDWDALVRRGIFLYPTNWGEGPAGTVGGED
                     IFQIAEVTNGSPHIYGLWPHAASLFLRGPGTRAERDRPAADAPAHPAGKNRRKSASGW
                     DSARGRLVIDTPYTQGIAGSLDGESASFAHIDFETNNSFAVLVATSVSNQPIATTKRL
                     LVSAIARVEPTGFRWVDSWKREVADPGRPPFLQEPVSAKIVWRRKGAARAFVLDNNGA
                     RIGEAKLESRPGGDGVSLVIDGKTAAFHWELTAE"
BASE COUNT          813 a         1194 c         1236 g          786 t
ORIGIN      
        1 aggatgatca gctcgcaaac gccgacgctc ttgctcaagg acggtcgagc tcgcgtgatc
       61 actggctcgc cgggcgggcg cactatcccg aatacgacgt tgtggatcgt gctcaatctg
      121 gtcgagattg ggttgggacc aaacgaggcc gtccaagcac cccggactca tcatcagtgg
      181 ttccctgacg tgctgacgct ggaaggtcgc tcgtgggccg atcccacccg catggcactc
      241 actgccatgg ggcacaagct gagaacgatc gagcaccaag ggaatgcgaa cacgatcgtc
      301 gtcgatcccg aaggccgcgt gcacggaatc gccgatccga ggcggtcgac gacaaaggcc
      361 tcgggcgatt gaggcacaat cgacgcccgt tccacgcgtg ttgattttcg aaactccgca
      421 ctaatcgcgg cgcggcacac gcttcgtttc tcggcgtagt aataaggagt gacgccgttg
      481 cactcgtgac ctgaccccta aagcgatatg tgtcaggcac gatttccgcg aacgccatac
      541 ctatcgaatc cgagctaggc ttgctctaga acgggcgaat cccgcatttt ccagtggagt
      601 tgaccatgat ggcgagtggt tcccattcgg atagtccaac gatcgtgttg cgcattagcg
      661 atggaacttt tcacatgcat cgagttttca acgatttcgc tcaggctagt cgaagcgctg
      721 aagcctatgc cggagcgggc ttcgctgtcg cgatgatgtc ggcgacaggc cggtttttga
      781 tgggttttga ggcacgtcgt caacggctag ctatctgatt ctggccaacc ggcaccgacg
      841 ctcttgatcg gtcaatgcgc gctttttccg ggaaaaacag ggtaaaacgg ggccctcatt
      901 cgaaaaaaac cgggaaactg tagccaaaat cgcggttttg acctgatttt gctggatgtt
      961 ttcggcataa tcgtcgaata cgaacaccag gagcctcgat tttccccttc aggagaaggg
     1021 ggaaagacca actggttcgt atggtccgta ccagtcgagg cagcgaaatc acacaggagg
     1081 gcttagtttc catggccaag aaagccgcac ccggcaccac cgctcccaag gcggcccccg
     1141 caaccggatc gtcgagcaaa ccggcgacga agggggagat ttacaccaag ctggcaacaa
     1201 agacgggact ttcgaagaag caggtttcca cggtatttga tgagctgacc gagctaatcg
     1261 gcaacgagct tggcaagaaa ggccctggac tttttgtggt ccccggtctt ctcaagctga
     1321 agatcgtccg caagccggcc accaaggcca agaccgggat caaccccttc accaaagagc
     1381 ctatgaccta caaggccaag ccggcacgca atgtggtcaa ggctctgccg ctgaagtcgc
     1441 tcaaggaaat ggtctagacc gattcagctc accatccggt gactttgaag tccctatttt
     1501 gcgagaatca cggaaaaccg cgccggtcga ccaaaccaat ggtcgacgcc ggcgcggttt
     1561 ttcatttgga gttggctggt gcgttcgatg gggacaggaa gatcggcttt atcgaggcaa
     1621 aaccggacgc gaccgatacc actggacgta ccgcctgatg ccttcgccga acggtgtcga
     1681 gggggcgtat ccgagttcac tcctggcccg gtcgatggcg gcgaaggttt gtttgacgtc
     1741 acccggctgc tcgggcaggg cttgaatgac tgccggcttt cccaacgcga gcgcaatcgt
     1801 atcgatcatc tgtttgagct cgataggctc ggagtgtccg aggttataga ggtgatgtcc
     1861 ggtacaacgc tcgatcgcgc gacaaatgcc gtcgacgatg tcgtcgacat acgtgtaatc
     1921 gcgccgggtc gtgccgtccc cgaacatagg gactggctgg ttctgatcga tcaatcgggt
     1981 gaatttggcg atggccagat cgggtcgatt gcgcggtcca aagaccgtga acaagcgaag
     2041 gccgctgacc ggcaggccat ggatgtgatg gaatgtgtgc gccaagagct cgcacgcttt
     2101 cttggtcgcg gcgtacgggc taacaggcag gtcgaccgtg tcggtctctc gaaacggccc
     2161 ttctgcgcgg tcgccataca cactcgagct cgaggcgtaa acgaaccgcg gccgcggctc
     2221 gagccggctc gcggcctcga gccagacgac ggtccctaca acgttgacat cagtgtataa
     2281 gcgagggtcg tctatgctgg gccggacgcc tgcccgcgcc gccagatgga cgatcacctc
     2341 tgggcgggtt tcggcgacca gagtgtctac gcctgccgcg tcgcggatgt cgagttccgc
     2401 cagacggcat cgtgggctgc cgagcgcgtt gttcaggttg gcttccttgg tggcgcgctc
     2461 gtaaaacggg tcgaagttgt cgacgacgat gacttcattg ccttccgcga gcagtcggtc
     2521 aacgagatgt gaaccgataa agcccgcccc gcctgtgaca atgattttca aagattactc
     2581 cgcggtcaat tcccaatgaa acgcggccgt cttgccgtcg atgacgagcg agacgccgtc
     2641 accaccggga cgcgactcaa gtttggcctc accgattcgt gcaccgttgt tgtcgagcac
     2701 gaaggctctc gctgctccct tgcgccgcca gacgattttt gccgataccg gctcctggag
     2761 aaacggtgga cgaccggggt cagcaacctc gcgcttccac gaatcaaccc agcgaaagcc
     2821 cgtaggctcg acgcgcgcta tcgccgagac gagcaaccgt ttcgtcgtgg cgatcggctg
     2881 gttgctcaca gaggtggcaa ccagaacggc gaacgagttg ttggtctcaa agtcgatatg
     2941 cgcgaacgac gcggactctc cgtcgagcga tccagcgatt ccctgggtgt acggagtatc
     3001 gatgaccaga cggccacgcg cggagtccca gccggaggcc gattttcgcc ggttttttcc
     3061 ggcaggatgt gccggcgcgt cggctgccgg ccggtcgcgt tcggctcgcg tacctgggcc
     3121 gcggaggaac agcgacgccg catgcggcca gagcccgtag atatgtgggc tgccattggt
     3181 tacctcggcg atctggaaga tgtcttcccc cccgactgta cccgccgggc cttcgcccca
     3241 gttggtcggg tacaagaaaa tcccccgcct cacgagcgcg tcccaatccc cggctaacgc
     3301 gctataaacg cccagaaact ggtcggccgc ctcgtgagga aatgaccagg ctccctgcgt
     3361 aacgttgcat gtctgcccaa ttacataggg ccggtcgggc ctgcgtttca tcgctgcttg
     3421 ggccgcgaga ctcgtatccg ggccgcacca gagcagcgag cgtttttcgg gagacaccca
     3481 gagcgcgggg ttccagaaga gacgatcgtc gatcaggtcg agaccaggtc ccgcctgggc
     3541 cgcgccaaat tctggctcgc gccgccagtg cgagaccccg gcgatcggga cgcggagacc
     3601 gtcttttcgc aaggcttccg ccatcctttt agagtgggcc gcctccacgg attcccagaa
     3661 ccgacgaccg ggcccgccct tggccttctc tgccaggctt tgaagggcct tggcgtacgc
     3721 agcgggcagc gcgtcggggt tgtcgatcag attgaaaagc gtcacttcgc ccgtcaatgt
     3781 gacccatgcg aggacaggat catccttgag cgccaggccg gtttctggat tgacatgtcc
     3841 caggagggcc ctggctgagt cgatcgcgag cttgctcaag gtcgggtcga acaaagccgc
     3901 gggaccgccg ccgggaggta gcatgccggt tgcggtcact ccatcgccgg tccggaatct
     3961 tcgtttgccc tgaagctcga gcgccacgaa gatgccgcga gacttgagcg cggcgatgag
     4021 atggtcaag
//
LOCUS       DATAQL010000340         9180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_33864, whole genome shotgun sequence.
ACCESSION   DATAQL010000340 DATAQL010000000
VERSION     DATAQL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33864"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1087
                     /locus_tag="VHS97_05805"
     CDS             80..1087
                     /locus_tag="VHS97_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX3447746.1"
                     /translation="MCIAYTHLELRPALDDILRSHDANLGTIVRLESNRCADQTSFNL
                     VDLEIELEDGTVLVLLLKDLGMENLHETARRVKPHFLYNPLREIKTYQEILAPSGLST
                     AHFYGAVVRPDKDRYWLFLEKISGLRLSQVSEYSAWLQAARWLARLHYRFAGESDTLS
                     RSVPLLHYDAAFYRTWLERVEEFQSVGPRKTVWQALAGNYDRAIERLIRLPSTLIHGE
                     FYASNILIQETREGCRIRPVDWETTAVGPGLIDLAALTAGDWTECERAEMAAAYHDTL
                     LSEGMPGFPLEDLLLALDDCRLHLAIRWLGWAPDWSPPSENAHNWLEEAIALSEKMGM
                     R"
     gene            1084..1851
                     /locus_tag="VHS97_05810"
     CDS             1084..1851
                     /locus_tag="VHS97_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX3447747.1"
                     /translation="MKACAHRGGKTPIPFSVRHTMDSTRFLIVNADDFGLSEGVNRGI
                     IEAHERGIVTSASLMVHKPSAISAAAYSREHLRLGLGLHLDLGEWAYDGEAWTPVYRV
                     VPLDDYSVVTEELERQLSDFRRLVGRDPTHLDSHQHAHRYEPVRSAAHNLARRLNVPL
                     RHFSHGIQYCGRFYGQTARGVPLPEAISVESLIAILSDLPPGVTEVGCHPGRVTGDEL
                     TYGSERSTEVDVLSHPRVKALVTSLGIELCSFQEVMR"
     gene            2237..5161
                     /locus_tag="VHS97_05815"
     CDS             2237..5161
                     /locus_tag="VHS97_05815"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3447748.1"
                     /translation="MSTIGLALAGMILALNWTSCPASADDGKKGLTVGESISPEAAEF
                     FEARIRPILADQCVKCHGPKKQSSGLRLDSRAAALKGGDSGPAVVPFKPEESLLLQAV
                     EHTRDELKMPPERKLADSSIAMLRQWITMGAPWSADSMEKKTGTAASNAAASSRHWAF
                     QPVKPQAPPPVKERDQVRTPVDSFVVAGLEAAGLTLSLPADRRTLIRRATLDLWGIPP
                     TAEEVEAFAADTSADAFERLVDRLLASPRYGERWGRHWLDVARYADTKGYVFSQDRRY
                     PYAYTYRDYVIAALNSDMPYDQFIIQQIAADQLPRGENVRPLAAMGFLTVGRRFLLDQ
                     NEIIDDRIDVVTRGILGLTVTCARCHDHKYDPIPTEDYYSLYGVFASSTEPAELPLLT
                     RPGHASHFADYDRKLKAASKALDDYLATRREELAADLKARFSWYFKAAHDLGFDAGNQ
                     NLDERALADKLNSRRLKAVIALWNRRPESARKPDDPVFGPWNAFEAIPKEQFTAKAGD
                     VYRKMKASCAANAETMHPLVAETVLTGPPASMNEVLERYATLFGELEARWEENATRNV
                     TADDLPDAEWESLRHALFGTSSPLFVSTDLMREFLDQTQNEELNQLGGAIEELNATHP
                     GAPSRAMVLYDAAQPVEPRVFIRGNPGRPGPAVPRRFLRLLGESDRRPFQKGSGRLEM
                     ARAIADSKNPLTARVLVNRTWLWHFGKGLVATPSDFGLRSEPPSHPELLDYLASEFIA
                     SGWSQKSLHRLIMLSSTYRQRSDPREACVNRDPENRLLWRFNRQRLDFESMRDSILAV
                     SGALDPSIGGPATLLTEPPFSTRRTLYGFIDRQNLDGLYRTFDFAVPDATSPRRFVTI
                     VPQQALFLMNSPFLHEQARRLASSAGLDSRSVSTAGPAVSAFDQVEGVRRLYRRVLGR
                     TPETDELLLAIEFIGRQTDSPTNDTHAWKPAQNEKSDHPLAPWEQLSQVLLLTNEFMY
                     VD"
     gene            5169..6662
                     /locus_tag="VHS97_05820"
     CDS             5169..6662
                     /locus_tag="VHS97_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447749.1"
                     /translation="MSHQPPRLEDAIVTRRELLCRSGMGMGALALGNILAEKGLLGQA
                     ARAADAETGPIGPALSTIGAANPLAPKASPLQARAKRVVHLFMNGGPSHVDTFDPKPT
                     LDRYNGKPVPTNLPTERKTGAAMGSPFRFNKYGQSGIEVSELFAHTAGMIDDICVIRS
                     MHADVPNHEPSLMLMNCGESRQARPCVGSWVLYGLGTENQNLPGFIVMCPGGFPIAES
                     QNWQAAFLPGIYQGTYLDSKHTEIEKLIAHIRNRGVGPSAQRAQLDLLAELNRRHLDG
                     RKGEAELEARIQSFELAYRMQSEAADAFDITQEPESIRAMYGPGVQGRQLLATRRLLE
                     RGVRFVQLWHGEGQPWDHHDDLATGHRELAKQCDRPIAAFLKDLKQRGMLDDTLVIWG
                     GEFGRTPTVELPTPGSNQGKMNGRDHNHHGFTMWMAGGGVKGGYVHGATDELGFKAVE
                     NKVHVHDLHATLLYLLGFDHEHFSYRYAGRDFRLTDVHGSVIKDIIA"
     gene            6939..>9180
                     /locus_tag="VHS97_05825"
     CDS             6939..>9180
                     /locus_tag="VHS97_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447750.1"
                     /translation="MHNIVVRSCPFLKLHRSPRPSAWIGGLFGLVVLSALIAPAELRA
                     QPAARKAASEAAPAELTPLGRYVPKDNLIFYMENEGLTAHAASWEKTAAYKMLNGTPL
                     GVMLEEVCGQLLDKAMTFVPNHRLSGPEIVSLFKGASKSGWVLALNLNPKGPDHVRGT
                     FVQRGAVSKELKPISSKLMGWMMGTNPKYKIDKKDGRTIVVLSAGSAATGPAAVLNAG
                     WAWWAENDDLVVNFMSPAGVDAVIAALDGKTAASIDHPVINELKKPEGTFQPVCVAFA
                     ETANCPEAPTKLVELLRSLKAEHGLDRIDLRWGFDDEALMSVSRLVAPKPRKTSLAFL
                     DQPTFKKDSLLPMPDSVESFVELSINTNTLVESLEHLGPPGTVKAKVDELAETIKKSG
                     QIDLRKDVLSHLGPRMVAYLASGRPAATNDDSLEAAFKNGLSATAAVTAMQTLFPKLT
                     LVAEVKNPEAFGKALDTVVIAINNEIKAQAIQKAAEDKAAADAAEPGGAARTPGGRGA
                     GGGDATKRRREPTYPRFNLIPGQTKSFVLMTPSDSALRFGPSSFRPTILLEDKYVAFG
                     VSSDSVRAALTAVRRKDWKPSASLEKACEHVPPNLVVLSVTDVSESLSSMLASLPGTL
                     QTMINTSIALAKARASGTGTNAAPGNGPGRPSGMPGGGPMSGGSGSMRPMMRPGGPGG
                     ASRPGFGPQANSGSSSPGGTAGDSASGTTTENMIVLNVDPDKLPSAADLKAHLFPTTL
                     SVTVSDP"
BASE COUNT         1850 a         2831 c         2754 g         1745 t
ORIGIN      
        1 aactccgaca agattctcag tcgcctcctc gaccgcctct tcagcacttc agctgggact
       61 tggaatgcta gaaactgata tgtgcatagc atatactcac ctcgaattgc ggcccgcgct
      121 cgatgacata ttgcgcagcc acgatgcgaa tctcgggacg atcgttcggc tagaaagtaa
      181 ccgatgcgcc gaccagacaa gcttcaacct cgttgacctt gagatcgagc tcgaggatgg
      241 tactgtcctg gtgctcctgc ttaaagattt ggggatggag aatctccacg agaccgctcg
      301 tcgggttaag ccccactttc tgtacaaccc gctccgcgaa attaagactt atcaggaaat
      361 cctggctccg agcggcctta gcacggcgca tttctacggg gcggtcgtcc gaccggacaa
      421 ggatcgttat tggcttttct tggagaagat ctcgggcttg aggctttctc aggtaagcga
      481 atactccgca tggctccaag cggcccgctg gctggcgcgc ctgcattacc gcttcgccgg
      541 cgaaagcgat accctgagcc gatcggtccc tctcctgcat tacgatgccg ccttttaccg
      601 aacgtggctc gagcgagtcg aggagtttca atccgtcggg ccccggaaga ccgtttggca
      661 agcattggcc ggcaactatg atcgggccat tgaacggttg atccggctcc catcgaccct
      721 gatccatggt gagttctatg cttcgaacat cctcatccaa gagacgcgtg agggttgtcg
      781 gatccgtcct gtggactggg agacgacggc cgtgggaccc ggcctcattg acttggcggc
      841 actcacggcg ggagattgga ccgagtgcga gcgagcggag atggcggccg cctaccacga
      901 caccctgctc tccgagggca tgcccggatt cccactcgaa gacttgcttt tggccctcga
      961 cgactgccga ctccacctcg ccatccgatg gttaggatgg gcgcccgatt ggtcacctcc
     1021 gtcagagaat gcgcataatt ggctcgagga ggcgatcgcc ttaagcgaaa agatgggcat
     1081 gagatgaaag cttgtgcaca tcgtggcggc aagacgccga tcccattcag cgtgaggcac
     1141 accatggatt cgacgcggtt cctaattgtc aacgccgacg atttcggctt gagcgagggc
     1201 gtgaaccggg ggattatcga ggcccacgag cggggtattg tcacgagtgc cagcttgatg
     1261 gtacataagc catccgctat ttcggccgcc gcctacagtc gcgaacatct ccgattaggt
     1321 cttggcctcc acctcgatct gggcgaatgg gcctacgacg gcgaggcctg gactcccgtg
     1381 taccgggtcg ttcccctcga tgattactcg gtcgtgaccg aagaactgga gcgccaactg
     1441 agcgactttc gccgactcgt cggtcgcgat cctacacacc tcgattcgca ccaacatgcc
     1501 catcggtatg agccggtgcg gtcggccgct cacaacctgg cccgcaggct caatgtcccg
     1561 ctgaggcact tcagccacgg cattcaatat tgtggccgct tctatggaca gacggcgcgg
     1621 ggcgttccac tgccggaagc gatttcagtc gagtcgctca ttgcgatcct gtcggatctg
     1681 cctcccggtg tgaccgaagt cggctgccat cctggccgag tgaccggaga cgagttgacg
     1741 tatggctccg agcggtcgac cgaggtggac gtcctgtccc atcccagagt caaagctctg
     1801 gtgacttctc tgggtatcga gctctgctcg ttccaggagg tcatgaggta gttcccgcaa
     1861 aacgatcccg aggaccctgc gtccccgcgt atgacctaag attgaacaaa gttccgtaaa
     1921 tcctgaaatc gcacagtcag attttgtcga ggattccggc gacacagggg ttcccgccga
     1981 atcaacgggg gttgccagat agtcccccct gggaaataat gccactagcg ttattcggca
     2041 aacggctgat ggaacggcgg cgggccgaac cgggttcaga ccttaacgtg attttacgat
     2101 cctgccccct tgacccttct gaccatcgat ccatcaggcc acgagtctaa gaggccagtg
     2161 gaatcataag actatggctg tccgtctctt caggcgaaat acctgggttc gatggagatc
     2221 gcagcggact gaccgcttgt cgacgatcgg gctggcgctg gccggaatga ttctcgctct
     2281 gaattggaca tcttgcccag cttcggccga cgacgggaaa aaagggctga cggttggtga
     2341 gtcaatctcg ccagaagccg ccgagttctt tgaagctcgc atacgcccga tcctcgccga
     2401 ccagtgtgtg aagtgccacg gtcccaagaa acaatcgtcg ggtctacggc tggacagtcg
     2461 tgcggcggcg cttaaagggg gcgattcggg gcctgcggtc gtgccgttca agcccgaaga
     2521 gagtctgctc ttgcaggccg tcgagcacac gcgcgacgag ttgaagatgc cgcctgagag
     2581 aaagctcgcc gattcgtcga tagcgatgct gcggcaatgg attaccatgg gtgccccttg
     2641 gtccgcggac tcaatggaaa agaagacggg caccgctgcc tcaaatgctg cggcttcgtc
     2701 tcgtcactgg gcctttcaac cggtaaagcc tcaggcgccg ccgccggtca aagagcgcga
     2761 ccaagtacgt acgcccgtcg attcgtttgt agtcgccggg ctcgaggccg cgggtttgac
     2821 actctcgctg ccggcggaca ggcggaccct gattcgacgt gcgacgctcg acttatgggg
     2881 cattccgcct actgccgagg aggtcgaagc gttcgcggca gacacgtcgg ccgacgcatt
     2941 tgagcgtctg gtcgatcggc tcttggcctc gccacgctat ggagagcgct ggggacggca
     3001 ctggctcgat gtggcgcgat acgctgacac caagggctac gtcttctcgc aggaccggcg
     3061 ctatccgtac gcgtacacgt accgcgacta cgtcatagcc gcgctcaact cggacatgcc
     3121 ctacgatcag ttcatcatcc agcagatcgc cgccgaccaa ctcccccgtg gcgaaaacgt
     3181 cagaccgctg gcggcaatgg gttttttgac ggtcggccgg cgctttctgc tcgatcaaaa
     3241 cgaaattatc gacgaccgaa tcgacgtcgt gacccgtgga atcttgggcc tgacggtcac
     3301 ctgcgcgcgc tgccacgatc acaagtacga cccgatcccc acggaagact attattcgct
     3361 ttacggagta ttcgccagct cgaccgagcc ggccgagctg ccgttactaa cccggcctgg
     3421 gcatgcctcg catttcgccg actacgaccg aaaattaaag gccgcgagca aagcactcga
     3481 cgactacctg gcgacgcgtc gcgaagaact cgcagctgac ttgaaagcgc ggttctcgtg
     3541 gtatttcaaa gccgcgcacg accttggctt tgacgcgggc aaccaaaatc tcgacgagcg
     3601 tgccctggcc gacaagttga attcgcgtcg actcaaggcg gtgatcgcac tctggaatcg
     3661 ccgcccggag tccgcacgca agcccgacga ccccgttttc ggcccgtgga atgcgtttga
     3721 ggcgatcccc aaggaacagt tcaccgccaa ggccggcgac gtgtaccgca agatgaaggc
     3781 atcctgcgcc gccaacgccg aaacaatgca tccgctagtc gcggagaccg tgctgacggg
     3841 cccacccgcg agcatgaacg aagtcctcga gcgatacgcg actctgttcg gcgaactaga
     3901 ggcgcgctgg gaagagaatg cgacgcgaaa tgtgaccgcc gacgacttgc ccgacgcgga
     3961 atgggagtcg ttacggcatg cactctttgg cacgagcagt ccgctattcg tttcgaccga
     4021 cctgatgcgc gagtttcttg accagacgca gaatgaggag cttaaccaac ttggcggagc
     4081 aattgaggag ctcaacgcaa cgcaccccgg tgctccgtct cgcgctatgg tgctctacga
     4141 cgccgcgcag cccgtcgaac cgcgagtctt catccggggc aaccctggcc ggcccggccc
     4201 cgcggtgccg cggcgatttc tgcgattgct cggcgagtcc gatcgccggc cgttccagaa
     4261 gggtagtgga cgactagaga tggcccgggc gatcgccgac tcgaagaacc cactaaccgc
     4321 gcgcgtgctg gtcaacagaa cctggctctg gcatttcggc aaggggctgg tcgctactcc
     4381 aagcgacttc ggcctgcgga gcgaaccacc gagccatccc gagctgctcg actatctggc
     4441 cagtgaattt attgcctcgg gctggtcgca gaaatcttta cataggctga tcatgctctc
     4501 gagcacgtat cgccagcgca gcgacccccg cgaagcttgc gtgaaccgcg accccgaaaa
     4561 ccgcctgctg tggcggttca atcgccagcg gcttgatttc gagtcgatgc gcgattcgat
     4621 cctggccgtt tccggtgcac tcgatccctc gattggagga ccggccacgc tcctaaccga
     4681 gcccccgttc tccacgcgcc gcacgcttta cggcttcatc gatcgccaga atctggacgg
     4741 cctctatcgg acgttcgact ttgccgttcc tgacgcgacc agcccccggc ggttcgtgac
     4801 gatcgtgccc caacaggccc tcttcctgat gaatagcccg tttttgcacg aacaagcacg
     4861 ccgtttggcg agctcggccg gtctcgattc aagaagtgtc tcgaccgccg gcccggcggt
     4921 atcagcgttc gaccaggtgg aaggggtgcg ccggctttat cgtcgcgtcc tgggccgtac
     4981 gcccgagacc gacgagctct tgctggcgat cgagttcata gggcggcaaa ccgactcgcc
     5041 gactaatgat acccatgcgt ggaagccggc tcagaacgaa aaaagtgacc atccgctggc
     5101 tccatgggaa cagttgagcc aggtgttgct cttgaccaac gaatttatgt acgtggatta
     5161 acatcaccat gtcgcaccag ccaccacgac tcgaagacgc catcgtaact cggcgcgagc
     5221 tcttatgccg gtcgggcatg gggatgggag cactcgcgct ggggaacatc ctggcggaaa
     5281 agggcctcct gggccaagcc gcccgggcgg ccgacgcgga aacggggccg atcggtcccg
     5341 cgctttccac cattggcgcc gccaatccgc tggcacccaa ggcgtcgccg ctccaggctc
     5401 gggccaaacg ggtcgtccat ctgttcatga acggcgggcc gtctcatgtt gacacttttg
     5461 atcccaagcc aactctcgac cgctacaacg gcaagccggt cccgacgaac ctgcccaccg
     5521 aacgcaagac cggcgccgcc atgggctctc cgttccgctt caataaatat ggccaaagcg
     5581 gcatcgaggt cagcgagctg tttgcccaca ccgcgggtat gatcgacgac atctgcgtga
     5641 tccgctcgat gcacgccgac gtacccaacc atgagccgtc gctcatgttg atgaattgcg
     5701 gcgaatcgcg ccaggctcgc ccgtgcgtcg ggtcatgggt gctctatggc ctggggaccg
     5761 aaaaccagaa tctacccggc tttatcgtga tgtgcccagg cggatttccg atcgccgaat
     5821 cgcaaaactg gcaagccgcg tttctccccg gcatctacca aggaacctat ctcgatagta
     5881 agcataccga gatcgaaaag ctgatcgcgc acatccgtaa tcggggagtc gggccaagcg
     5941 cccagcgtgc ccagctcgac ctcttggccg agctcaaccg ccgtcactta gacggccgta
     6001 agggagaggc tgagctggag gcgcggatcc aatcgttcga gctggcgtac cgcatgcaat
     6061 ccgaagcggc ggatgcattc gatatcaccc aggaacccga atcgattcgc gcgatgtacg
     6121 ggcctggagt acaggggcgc cagctattgg caacacgtcg gctcctggag cgaggtgttc
     6181 gcttcgtcca gctatggcac ggcgaaggcc agccctggga ccatcacgac gacctcgcaa
     6241 cgggccaccg cgagctggca aaacagtgtg accggcccat cgcggcgttc ttgaaagact
     6301 tgaaacagcg cggcatgctg gacgacacgc tggtgatctg gggaggtgag tttggccgca
     6361 cgccgactgt cgagctgccg acgccgggct ccaaccaggg caagatgaac ggtcgcgacc
     6421 acaatcatca cggattcacc atgtggatgg ccggcggcgg agtcaagggg ggatacgtcc
     6481 acggcgcgac cgacgaattg ggtttcaagg ctgtcgaaaa caaggtccac gtccacgacc
     6541 tgcatgccac tcttctttac ctcttaggct tcgaccacga acacttctct tatcgctacg
     6601 cgggccgaga cttccgcctg accgacgtgc acggcagcgt catcaaagac attattgctt
     6661 gaaacgccag gtctggtctc tcacactcac atcctgaagg cagcttggtc gatcccggcg
     6721 ggcaggctgt tggcgcggcg gcgtctggct cttacgatga gtgcgggctg gcacacgaac
     6781 tcgcgggcgt gcggcgaacg atcttttgca tgccaggcgc ggcccgcgac tgcttcgagg
     6841 tcgataccgc cgcgcgacgg ccaagttttt cgggatgtct aatgcgcgta tgcgtccttg
     6901 gtcatgctct ccagtcgaac ggtacgggat gaactgaaat gcacaatatt gttgtccgct
     6961 cgtgtccgtt cttaaaactg catcgttccc cgagaccatc ggcctggatc ggcggcctgt
     7021 tcggcctcgt cgtgctgtcg gcgctgatcg ctccggcaga gttgcgggcc cagccggcgg
     7081 cacgcaaggc ggcgtccgaa gctgccccgg cggagctaac gccgctgggt agatatgtcc
     7141 ccaaggacaa cctcattttc tacatggaaa acgaggggtt gactgcccac gccgcgtcct
     7201 gggagaagac agccgcttac aagatgctga atgggacacc gctgggcgtc atgctcgagg
     7261 aggtatgcgg ccagctcctc gacaaagcca tgacgttcgt gcccaaccac cggctgagcg
     7321 gcccggagat cgtcagtctg ttcaagggcg cgtccaagtc gggctgggtt ttggcattaa
     7381 acctcaatcc caagggcccc gaccacgtgc ggggaacgtt cgtccagcgg ggcgcagtga
     7441 gtaaagagct caagccaatc tccagcaagc tcatgggctg gatgatggga accaatccga
     7501 aatacaagat cgacaagaaa gacgggcgga ccatcgtggt cttatccgcc ggatcggcag
     7561 ccaccggtcc agccgcggta ctgaacgcgg ggtgggcgtg gtgggccgag aacgacgacc
     7621 tggtcgtgaa tttcatgtcc ccggccggcg ttgacgccgt gatcgcggcg ctcgatggca
     7681 agaccgccgc ttcgatcgat caccccgtta tcaacgagct caaaaagccc gagggaactt
     7741 ttcaaccggt ctgcgtggct tttgccgaga ccgccaattg ccctgaagcc ccgaccaagc
     7801 tggtggagct cttgcgtagc ctgaaggccg aacatggcct cgaccggatc gatcttcgtt
     7861 ggggctttga cgatgaggcc ttgatgtcgg tcagccgctt ggtcgcgccc aagccgcgga
     7921 agacaagcct tgcatttctc gaccagccga cattcaagaa agactcgctt ttgccgatgc
     7981 ccgactctgt cgagtctttc gtcgagttat cgatcaatac taacactctg gttgagtcac
     8041 tcgaacattt gggaccgccg gggacagtca aggccaaggt cgatgagttg gccgaaacga
     8101 tcaagaaatc gggccagatc gatctacgaa aggatgtact cagccacctt ggtcctagga
     8161 tggtcgccta tctcgcttcg ggacgacctg cggcgaccaa tgacgattcg ctggaggccg
     8221 cgttcaaaaa cggcctgagc gcgaccgcgg cggtcacggc catgcaaacg ctctttccca
     8281 agctcacgct agtggccgag gtaaagaatc ccgaagcctt cggtaaagcc ctggacacgg
     8341 tggtgatcgc gatcaacaac gagattaaag cccaagcgat tcaaaaggcg gctgaggaca
     8401 aggcggcggc cgatgcggcg gagcctggcg gcgccgctcg cacgccggga gggcgcggag
     8461 ccggcggagg agatgcgacg aaacggcggc gcgaaccgac ctatcccaga ttcaacctga
     8521 taccaggcca gacgaagtcc tttgttctta tgactcctag cgattccgcg ctgcggtttg
     8581 ggccttcgag ctttcgtccc acgattctgc tcgaggacaa gtatgtcgcg ttcggcgtgt
     8641 cgtcggactc agtgcgggcg gccctgacgg ccgtgcgccg caaggactgg aaaccctctg
     8701 ccagcctcga gaaagcctgt gagcacgttc cgccaaacct ggtcgtactc agcgtgaccg
     8761 acgtgagcga gagtctttcg tcgatgctgg caagcttgcc gggcacgctt cagacgatga
     8821 tcaacacgtc gatcgcactg gccaaggccc gtgccagcgg cacgggcaca aatgcggctc
     8881 cgggaaacgg ccctggaaga ccctccggaa tgcctggcgg cggtccgatg tcgggcggga
     8941 gcggttcaat gcggccgatg atgcgaccgg gtggccccgg cggggctagt aggcccggtt
     9001 tcggccccca ggcaaacagc ggctcaagct cacccggagg gacggcagga gactccgcaa
     9061 gtggcaccac caccgaaaac atgattgtgc tcaatgtcga tcctgacaag ctccccagtg
     9121 ccgccgatct caaggcccat ttgtttccga cgactctgtc agtcaccgtg agtgaccccg
//
LOCUS       DATAQL010000341         7649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4461684, whole genome shotgun sequence.
ACCESSION   DATAQL010000341 DATAQL010000000
VERSION     DATAQL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4461684"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VHS97_05830"
     CDS             complement(<1..384)
                     /locus_tag="VHS97_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447751.1"
                     /translation="MNRDHPLLALKPNMQTHRFALAQDEGLAKRQVFHNNGICWPAFN
                     LECRGDANFDESCSRKNRSFLDPMVDQEREHRGAELGFPAWVWCAERSIEQGVCSTAK
                     SIFARFERLTPVPLALPGIVRQVDQA"
     gene            370..4068
                     /locus_tag="VHS97_05835"
     CDS             370..4068
                     /locus_tag="VHS97_05835"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3447752.1"
                     /translation="MVSIHGRIHGRDDAWALHATGRIRERRGLTAPPASVSLEDLRNS
                     CPDPFAPESLYEKLRAVGLDYGPMFRSIEGLWLGNGEIIAAIELPHALERDIDRYHLH
                     PVLLDASFQALLSAALGIGADDRVLGGHLYLPVQISRVKFYTAARSRIWCHGRIREFN
                     SLNLIGDIQLFDEAGNLVADVRGFKCQSLSGRPCSDAERAREWLYDIDWHKLAAPESV
                     GRDCGAWDSHRLKDSGRFETLIFIDREGIGAALADELRRQEHSPIVVSQGGSRVQVDS
                     CHYQINPSRAEDMDWLLERVGSESFCRRIVYLWGLDIDTPACSVELSDKHAGLDDCVS
                     VLHLVQALARSPWKQSPRVWLVTRGAVQVDPRQMRVVPGQAALWGLGRVIDLEHPEMQ
                     CSLLDLDASAREPNVSRILEELVGDGPESELAHRGGVRYGLRVVRADKSRVDPERSAR
                     RVDSNASYRLELTRTGSFDSLFFREFARQDPGPGEVEVRVKATGLNYKDVLKVMGLLS
                     EEVVRDTWSGRALGLECAGVITRVGPGVSPARVGEEVQGWVADGFRTYITGRNEQFVP
                     RFEGLSSEEAAAVPVVFLTAYYGLKEIARLRRGERVLIHAATGGVGLAAIRLARALGA
                     EVFAMAGSPEKRAYLVGLGIQNVMDSRSLDFADQILERTQQRGVDVVLNSLTGEALAK
                     SLSILAPYGRFIEIGKRDIDADRALRLKPFNRNLLFAAIDVDRLLVDRPDDIARMQEE
                     LRDLFRQRLLSPLPITVYPAEEVESAFRLLAQARHIGKVVVSFESEAVTIAPPVKEPV
                     TIRRDGSYLITGGCGGFGLETARWLADRGAGALVLVSRRGAVTREAIEAVAALERRGV
                     QVMVAAVDVTDSARLAELLQTVASAFPPLSGVFHSAMVLDDDLIDRLDAARFRVPMSA
                     KVLGAWNLHAQTLDLPLDMFVLYSSMVAVLGNLGQAGYASANAYLDGLARHRRAMGLA
                     ALSINWGSLAEVGFVAHNPAISRHLERTGMFPIAASRALDMMGSLLGSDRAGIGVANL
                     DWSVWEHAVPALAARPRFTEVLVPRDDNEGASESESPRALIAAIRKAATEDRISLAAD
                     ILREDIAKVLRLPASKLDVDQNLNQLGIDSLMAVELQTLIAEKTGAHFSPMDFMAGPS
                     IMTMAKKLLEKLFPGEDVDSAGGATEIHVDHIPPSRIDYAASPVDQSLAGQPAQSLRG
                     LSIPDIDGLSEIELDELLSRITNQETHL"
     gene            4065..5480
                     /locus_tag="VHS97_05840"
     CDS             4065..5480
                     /locus_tag="VHS97_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3447753.1"
                     /translation="MNATHNNGHTRTVEEKRALLKRLLEENPVRAYNVSNRDVEQPPA
                     PVASRNDWDDIPVENYVFERSPEYLNLATQMDAAAAIGIEYPFFKVVEPINGAVARVN
                     GRQCINFSGYNYLGMSGDPVVSRAAKDAIERLGTSASASRLVAGQRPIHLELENALAD
                     HLGTEDALSFVTGYLTGVTTIGHLFKPGDLIVHDALIHNCTLIGSKLSGARTVAFPHN
                     DADALERILRELRGKHRRVLILAEGIYSMDGDIADLPRMIDLKRRFKAFLMVDEAHSI
                     GVLGRTGRGVTEHFGVDPREVDLLMGTLSKAMGSCGGYIAGNRALIDYLRHTVPGFVY
                     SVGMPPPVAAAALAALRLLRDEPQRIYRLRDLSRLFLLEARARGLNTGSSRDTPIIPV
                     IVGDSRRCGLLAQALYLRGINIQPIIFPAVEDRASRLRVFVTCLHTEREVTSTVDAVV
                     EELKRLGDQSLFRGRKNSESE"
     gene            5484..6314
                     /locus_tag="VHS97_05845"
     CDS             5484..6314
                     /locus_tag="VHS97_05845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3447754.1"
                     /translation="MERRGSGRFFEGKNIVLTGGSSGIGRSLAVALGCQRANLLLIGR
                     RQAQLDATVALLESTREDPAPIWETRAVDVSHREAIQSALRSHDEKRPVDVLINCAGI
                     AWVHYVDRMPPEAFEQSIQTNYLGTVWSSLALIPSFKSRRRGIIANVSSLAGVLGFIG
                     YAAYSPSKFAVIGFSEAIRNELRPYNVGVSLLLAPDTDTPQLEAENLTRPAETRAIAD
                     RARVLQPEHVARVLLKGIVAGKFWIVPGWDAQLIDHASRHFPGLTRWVLDRLVASGRR
                     "
     gene            complement(6376..7425)
                     /locus_tag="VHS97_05850"
     CDS             complement(6376..7425)
                     /locus_tag="VHS97_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447755.1"
                     /translation="MRSLWSIVGSLLICNVCAAQWTPGPSVTKARLRGLAVVNTKVVW
                     ASGNSGTFVRTDDGGLTWNAGQVAGALDLDFRDVHAFDDRKAFLLSIGDGPKSRIYQT
                     DDGGATWVLRFQNQDARVFLDAISFWDADHGIALGDPVDGCFTTLLTDDGGMNWKRST
                     SESMPHAKKGEGAFAASGTCLVVEGERNAWFGTGGADHARVFRSVDRGQTWSVYETPI
                     PAAQPSSGLFSVAFRDHDHGIAVGGDYKEPDKTGPVAALTSDGGRTWTLPKGKPPAGY
                     RSAIAYVPGASQPTAVAVGPTGSDLSTDGGESWSPLGKVGFHAVGFAGPIDAGWGVGD
                     NGLIARFRGSLGAKP"
     gene            complement(7440..>7649)
                     /locus_tag="VHS97_05855"
     CDS             complement(7440..>7649)
                     /locus_tag="VHS97_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447756.1"
                     /translation="VFVGMFDEVDEGTAIYKVAGQTPVGKYFVTYDGLPSDWYLRLTG
                     AATQMIRGDLPLSQKIPEKLPSPIE"
BASE COUNT         1429 a         2326 c         2313 g         1581 t
ORIGIN      
        1 agcctggtcc acctgccgca ctatccctgg cagcgcgagc ggcactggtg tgagtcgctc
       61 gaatcgcgcg aagatcgact tggccgtcga gcacaccccc tgctcgatcg aacgctccgc
      121 acaccagacc caagctggga agccgagctc agcaccgcga tgttcccgtt cttggtcgac
      181 catcgggtcc aggaaactcc ggtttttcct ggagcaggat tcgtcgaagt ttgcatcgcc
      241 gcggcactcg aggttgaagg ccggccagca gatcccgttg ttgtggaaga cctgtctttt
      301 cgcaaggcct tcgtcctggg ccagggcgaa tctgtgcgtt tgcatgttgg gtttgagagc
      361 caggagcggg tggtctcgat tcatggtcga atccacggta gggacgacgc ctgggcattg
      421 cacgcgacag gtcgaattcg cgagcgccgc ggcttgaccg cgccgccggc gagcgtgagt
      481 ctggaagacc tgcgcaatag ctgcccggat ccttttgctc ctgagtcgct ctatgaaaag
      541 ttacgcgccg tcggcctcga ttatgggccg atgttccggt ctatcgaggg attgtggcta
      601 ggtaacggtg agatcatcgc cgcgatcgag ttgccccatg ctctcgaacg ggatatcgat
      661 cgctatcatc ttcatccggt gttgctcgac gcgagttttc aggctctgct ttcggcggca
      721 ctcgggattg gtgccgacga ccgagttttg ggtggacacc tctacctgcc tgtccagatt
      781 agccgggtca aattttatac ggctgccagg tcgcgcatct ggtgtcatgg gcggattcgc
      841 gagttcaatt cacttaactt gatcggcgat atccagctct tcgacgaagc gggaaacttg
      901 gtggcggacg tccgcggttt caaatgccag tctttgagcg gccgtccttg ctccgatgcg
      961 gaacgggccc gagaatggct gtacgacatc gactggcaca agctcgctgc tccagagtcc
     1021 gtcggccgtg actgcggcgc ttgggattcc catcgtttga aagactcagg acggttcgag
     1081 actctgatct ttattgatcg agaaggaatc ggcgccgctc tggccgacga actgcgacgg
     1141 caggaacata gcccgatcgt ggtcagtcag ggcggctctc gtgtccaggt cgactcgtgc
     1201 cactatcaaa tcaacccgtc tcgcgccgaa gacatggatt ggctgcttga acgcgtgggt
     1261 tctgagtcgt tttgccgccg gatcgtttac ctgtggggac tggatatcga tacgccggca
     1321 tgctcggtcg agctatccga caaacatgcc ggactcgacg actgcgtcag tgtgttgcat
     1381 ttggtccagg ccctcgcacg atcgccatgg aagcagtctc cgcgggtatg gttggtcaca
     1441 cgaggtgctg ttcaagtcga tccgcgccaa atgcgagtgg taccgggcca ggccgcgcta
     1501 tggggactgg gccgagtgat cgacctggaa catccggaaa tgcagtgctc tttgttggat
     1561 ctcgatgctt cggcccggga gccaaatgtc tctcgtattc tggaagagtt ggtcggcgat
     1621 ggacctgaat ctgagctggc gcaccggggg ggcgttcgtt atggactgcg ggtggtacgc
     1681 gctgacaagt cacgcgtaga ccccgagcgg tccgcgcgta gggtcgactc gaacgcttcg
     1741 tatcgtctcg aactcacgcg aacgggtagc ttcgatagct tgttttttcg cgaatttgcg
     1801 cggcaagatc ccggtcccgg cgaggtcgag gtacgcgtga aggccacagg cctcaactac
     1861 aaggacgttc tcaaggtaat ggggcttcta tccgaagagg tcgtcaggga cacgtggtcc
     1921 ggccgcgcac ttgggctcga gtgcgccggc gtgatcacgc gcgtcgggcc cggcgtttcg
     1981 cccgcgcgtg tcggcgagga ggtccagggt tgggtagccg atggattccg aacctatatc
     2041 accggtcgga atgagcagtt tgtgcctcgg ttcgagggat tgagcagcga agaagccgct
     2101 gccgtgccgg tcgtattctt gacagcctat tacggtctca aggaaattgc ccggctccgg
     2161 cgcggcgagc gagtcttgat ccacgcggcg actggaggtg tcggactggc cgcgattcgg
     2221 ctcgcgcgag ccctcggcgc tgaggttttc gcaatggcgg gaagcccgga aaagcgggct
     2281 tatctcgttg gtttgggaat ccagaacgtg atggactctc gaagcctgga tttcgctgat
     2341 caaattctcg agcgcaccca acaacgtggt gtagacgtcg tcctcaattc actgacgggc
     2401 gaggcgcttg ccaaaagtct ctcgatactg gcgccgtacg gacgattcat cgagatcggc
     2461 aaacgtgaca tcgatgccga tcgcgcactg aggctcaagc catttaaccg caatcttttg
     2521 ttcgcggcaa tcgacgtcga tcggctgctc gtcgaccgac ccgatgatat cgctcggatg
     2581 caagaagagc tcagggatct cttccggcaa cgcctactgt cacctctgcc aattacggtt
     2641 tatcctgccg aagaagtcga aagtgccttc agactcttgg cccaggcgcg gcacatcggc
     2701 aaggtcgttg tctcattcga atctgaggct gtcaccatcg cgccgccagt caaggaaccc
     2761 gtgacgattc gtcgagacgg gagctacctg atcacgggag gctgcggcgg ctttggactt
     2821 gaaacagctc gctggctggc cgatcgaggg gcgggtgcgc tggtgctggt gagccgccgg
     2881 ggtgcggtca ctcgagaggc tatcgaggcc gtcgccgcgc tcgagcgccg gggcgttcaa
     2941 gtcatggtgg cagccgtgga tgtcaccgat tccgcgcgtc tcgccgagtt gctccaaacg
     3001 gtcgcgtcgg cgtttccacc actcagcggg gtcttccatt ccgcgatggt tctggacgac
     3061 gacctgatcg atcgcctcga cgcggcgcgc tttcgcgtcc ctatgtctgc caaggtgttg
     3121 ggcgcttgga acctgcacgc tcaaacgctg gatttgccgc tcgatatgtt cgttctctac
     3181 tcatcaatgg ttgccgtcct cggcaatttg ggccaggccg gctatgcatc agccaatgcc
     3241 tacctggacg ggctggctcg ccatcggcgt gcgatgggtc tggctgcgct atccatcaac
     3301 tgggggagcc tcgcggaggt gggcttcgtg gcgcacaatc ctgcgatttc ccggcacctg
     3361 gaacggaccg gaatgttccc aatcgccgct tcgcgagctc tagacatgat ggggtcgttg
     3421 ctcggaagcg atcgagccgg aatcggggtt gccaatctcg attggtccgt ttgggaacac
     3481 gcggttcccg ccctggcggc gcggccgagg tttaccgaag tgctcgtgcc tcgagatgac
     3541 aacgaaggtg cttcggaatc cgaatcccca cgggctttga ttgcggcaat tcgcaaggcg
     3601 gccaccgaag accgcatcag cttggcggcc gatattctcc gggaagacat cgccaaggtc
     3661 cttcgcctgc ctgcttcaaa gcttgacgtt gaccaaaacc tcaaccagct cggtatcgat
     3721 tccctcatgg ctgtcgagct gcagacactg atcgccgaaa aaaccggcgc ccacttttct
     3781 cccatggatt tcatggccgg cccgagcatc atgacgatgg ccaagaagtt gctcgaaaag
     3841 ctcttcccgg gtgaagacgt cgactccgcc ggcggcgcga ccgaaattca tgtcgaccat
     3901 attccgcctt ctcgaattga ctacgccgca tctccggtcg atcagtcgct tgccgggcag
     3961 cctgctcaat cgttgcgcgg cctctccatc ccagacatag acgggctctc agagattgag
     4021 cttgacgagc ttctctcccg gatcacgaac caggagacgc acctttgaac gcgacccaca
     4081 acaacggcca tacgcgaacc gttgaggaaa aacgggcact cttgaagagg ctgctcgagg
     4141 aaaacccggt tcgtgcctac aacgtctcca accgcgatgt cgagcagcca ccggcgcctg
     4201 tcgcttcgcg aaatgattgg gatgacattc ctgtcgagaa ttatgtgttc gaaagatccc
     4261 ctgagtatct caacctcgcg actcagatgg atgccgcggc ggccatcggc atcgaatatc
     4321 cctttttcaa ggtcgtcgag ccaattaacg gtgcggtcgc gcgggtcaac ggtcgtcagt
     4381 gcatcaactt ctccggctac aactacctgg gaatgtcggg tgatcccgtc gtttctcggg
     4441 cggcgaagga tgcaatcgag cggcttggaa cttcggcatc ggcgagccga ctggtggccg
     4501 gtcagcggcc gatccacctc gagcttgaaa atgccttggc cgaccatctg ggaaccgaag
     4561 atgctctgtc gtttgtgaca ggctatttga ccggcgtaac gacaatcggc catctcttca
     4621 aacctggcga cttgatcgtt catgatgcct tgattcacaa ttgcacgctc atcgggtcaa
     4681 agctctcggg ggcgcggacc gtggcgtttc cccacaatga tgcggatgcc ttggaacgga
     4741 ttctccgcga gctgcgggga aagcatcgca gggtattgat cttggccgag ggaatttaca
     4801 gcatggacgg cgatatcgcc gacctgcctc ggatgatcga tctcaagcgg cggttcaagg
     4861 cgttcttgat ggtcgacgag gctcactcga tcggggtcct gggccgcacg ggccgcgggg
     4921 tcacagagca cttcggagtc gatccgcgcg aggtcgatct cctgatggga acattgagca
     4981 aggccatggg gagctgcggc ggatatatcg cgggcaatcg tgccctgatc gattaccttc
     5041 gtcacacggt acccgggttt gtttacagcg tgggtatgcc gccgccggtc gcggccgctg
     5101 ccctcgccgc tttgaggcta ttgcgtgatg aaccgcaacg gatttatcgg ctccgagact
     5161 tgagccggct cttcctcctg gaggcaaggg ccagaggatt gaacacggga tctagccgtg
     5221 atacgcccat catccccgtg atcgttggcg actcgcgccg ctgtggtctt ctcgcccagg
     5281 cgctttatct ccgtggaatc aacattcagc cgatcatctt cccggccgtc gaagatcgag
     5341 catcccggct gagagttttc gtgacctgcc tccacaccga aagagaggtc acctcgacgg
     5401 tcgatgcggt cgtagaggag ctgaagcggc tgggtgacca gtctctgttc cgcgggcgaa
     5461 aaaactcgga gtcggagtga accgtggaaa gacggggatc cggtcgattc ttcgaaggga
     5521 agaatatcgt cctgaccgga ggctcaagtg gtatcggccg atcacttgcg gtcgccttgg
     5581 gctgccaacg agccaatctc ctcttgatcg gccggcgcca agctcagctc gacgcaacgg
     5641 tcgccttgct cgagtcgact cgcgaagatc ccgcgccgat ctgggaaacc cgcgccgttg
     5701 acgtgagtca tcgtgaggcc atccagtcag ctctccgttc ccacgacgaa aagcgccctg
     5761 tggacgtcct cattaattgc gccggcatcg cctgggtcca ctacgtcgac cggatgccgc
     5821 ccgaagcgtt tgagcagtcg attcagacca actacctcgg cacggtctgg tcgagcctcg
     5881 cgttgatccc atcgttcaaa agtaggcgcc gcgggatcat cgccaatgtg tcttctctcg
     5941 ccggcgtact cggtttcatc ggctacgcgg cctactcgcc gagcaagttc gccgtgatcg
     6001 ggttttccga agcgatccgc aacgagcttc gcccgtacaa cgttggcgtc tcccttctct
     6061 tggcccccga caccgacacg ccgcaactgg aggccgagaa cttgactcgg cccgcagaaa
     6121 cccgcgccat cgcggatcgc gcgcgtgttc tccaacccga gcacgtcgcc cgtgtccttt
     6181 tgaaagggat tgttgccgga aaattctgga tcgttcccgg ctgggacgcg caattgatcg
     6241 accatgcgag ccgccacttc cctggcctga cgaggtgggt actggaccgt ctcgtcgcgt
     6301 ccggtcgacg atgagttgca gcacgatcat tccgttcgtg ctcgcttcat cgcagatatg
     6361 tttcgcccgc gggcgctagg gtttcgctcc aagcgacccg cgaaaccggg cgatcaatcc
     6421 gttgtcgccg actccccagc cggcatcgat cggaccggcg aagccgaccg catgaaaacc
     6481 caccttaccg agcggactcc acgactcacc gccgtcggtc gagagatccg accccgtggg
     6541 accaactgcg actgctgttg gctgggatgc gcccggcaca tacgcaatgg ccgagcgata
     6601 accagccggc ggcttgccct tggggagcgt ccacgtgcgg cctccatcag acgtcaaggc
     6661 agcgacgggg ccggtcttgt cgggctcttt atagtcccct ccgacggcaa tgccatggtc
     6721 gtgatcgcga aatgccacgg agaagagccc cgacgacggt tgagcagccg gaatcggcgt
     6781 ctcgtagacg ctccaggttt gaccgcgatc caccgaccga aacacacggg catgatccgc
     6841 cccgcccgtt ccaaaccagg cgttccgttc gccttccacg accaagcagg tgccactggc
     6901 ggcaaatgcc ccttcgccct tctttgcatg cggcatgctt tcggacgtcg atcgtttcca
     6961 attcatgccg ccgtcatcgg tcaagagcgt cgtgaagcat ccatccaccg ggtcacccag
     7021 ggcaattcca tgatcggcgt cccagaagga gatcgcatcc agaaaaaccc tggcgtcctg
     7081 gttctgaaac cgaaggaccc atgtggcgcc cccgtcgtcg gtctggtaaa tgcgggattt
     7141 tggcccgtcg ccgatcgaca gcaagaacgc cttgcggtca tcgaaagcgt ggacatcgcg
     7201 gaagtcgaga tcaagcgcgc ccgcgacttg cccggcattc cacgtaagtc cgccgtcgtc
     7261 ggtgcgaaca aacgttcccg aattcccgct cgcccagacg accttcgtgt tcacgacagc
     7321 cagaccgcgc agccgggcct tggtgacgct gggcccgggc gtccactgtg cggcgcacac
     7381 attgcaaatc agcaaggacc ctacgatcga ccagagagac ctcataaacc ctccgccact
     7441 cactcgatcg gcgacggcaa cttctccggg atcttctgcg acagcggcaa atcgccccgg
     7501 atcatctgcg tggccgcgcc ggtcaatctc aggtaccagt cggagggcaa gccatcgtag
     7561 gtcacgaagt acttgccgac gggcgtctgg cccgccacct tgtaaatcgc cgtcccctcg
     7621 tcgacctcat cgaacatgcc gacgaagac
//
LOCUS       DATAQL010000342         4214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3240927, whole genome shotgun sequence.
ACCESSION   DATAQL010000342 DATAQL010000000
VERSION     DATAQL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240927"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..894
                     /locus_tag="VHS97_05860"
     CDS             85..894
                     /locus_tag="VHS97_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3447757.1"
                     /translation="MGRLIEPDAAPTRRLTFLGTGTSSGVPMIGCDCWVCTSSDPRNQ
                     RTRPSVLLSFERGNLLIDTTPEMRIQLLREKVRQVHAIAFTHHHADHLFGLDDARLFP
                     KWTGGPVPVYCEQDTEDTIRRAFSYAFRPGTEQWPPGFVPKLQFVRVAPGVEFDVLGE
                     RVMPIRLEHGKLPVLGFRVGALAYCTDVSRIPDESWPLLEGLDVLILDALRYEKHPTH
                     FSLSEALAVVEAVKPQRTFLTHLSHGFDHGPTQEKLPDRVVLAQDGLQLAF"
     gene            962..2386
                     /locus_tag="VHS97_05865"
     CDS             962..2386
                     /locus_tag="VHS97_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HEX3447758.1"
                     /translation="MSPDPILVDRLKQHGQSHLLKWWGELNEEEQGLLAAEIRSIDFE
                     RLDRLIADLVVSDRVTVPPADRVQPIDVIRLPQTDGERVVRRRAGGMGADALAAGEVG
                     VILVAGGSGTRLGYEGPKGTFPIGPVSSASLFQIHAEKIAALGARYGRPIPLYIMTSP
                     ENHEATIAFFESHDRFGLEHIRFFTQGQMPAVDRATSKVLLASRGRVALSPDGHGGTL
                     AALAAPGPHGTPSCLDEMRERGVRTLFYFQVDNPLVRIAEPSFIGLHREANAEVSFKV
                     VERTSPEEKLGVVVTVDGRPQVIEYSDLPAELAGRRLPEGPLELWAGSIAVHILERSF
                     IERLVGEHSLPFHRALKKVAFVDESGQNVKPAEPNAVKFEQFIFDVLPMAERWAMVET
                     DRAGEFEPLKNAVGPDSPATVHQRMSDQFGNWLEQAGAVIPRRPDGSVPFGIEISPLF
                     ALDANDLKSRIEPGLVIERPIYLR"
     gene            2861..3940
                     /locus_tag="VHS97_05870"
     CDS             2861..3940
                     /locus_tag="VHS97_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX3447759.1"
                     /translation="MLHAIIMAGGSGTRFWPKSRRDRPKQLLCLAGDTTMLQQTVARI
                     EPLVPRDRIIVVTGADQAAATRTQLPDVPAHNVIAEPSPRDTAPCVGLAAGIVAQRDP
                     KGTMIVMPADHVIEPAESFRATVVAAVAVIDDDPTALVTFGIKPTHPETGYGYIECGA
                     LLEKRNGIPVHRVIQFREKPDRDTAERFLAAGSFAWNSGIFVWRARTILDEIAAHRPR
                     LAQGLAPILKSIGTADEAETLARLFPGLERVPIDKAVMEHAPNVRVLEVPYRWNDVGD
                     WRALATLLDQDPAGNATQGKVVVRDTTGSIIISDDGGLVATLGVDDLVVVHSGKATLV
                     ARKDQLDKLKALVEGLGQAGYGSFL"
     gene            4079..>4214
                     /locus_tag="VHS97_05875"
     CDS             4079..>4214
                     /locus_tag="VHS97_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX3447760.1"
                     /translation="MRIIAGHRRGHKIDGPRASARTRPTSDLVRESLFNILGDLVVDR
                     T"
BASE COUNT          821 a         1241 c         1293 g          859 t
ORIGIN      
        1 atcatcgtga cttttggtat catctagcat tcgattgctc cggctccgag cgcgcgaaaa
       61 tcataattaa gcacctgggt agaaatgggt cgattgatcg aaccagacgc ggccccaacg
      121 cgacggctga cttttctcgg gacagggacg tccagcggcg ttcccatgat cggctgcgat
      181 tgctgggtct gtacgtcgag cgatcctcga aaccagcgca cgcggcccag cgtgctgctt
      241 tccttcgaac gcggcaacct gttgatcgac acgacccccg agatgcgaat tcagctcttg
      301 cgcgaaaagg tgcgccaggt ccacgcgatt gcgtttaccc accatcatgc cgaccacttg
      361 ttcgggctcg acgacgcccg cctgtttccg aagtggactg gcgggcccgt tcccgtttat
      421 tgtgaacagg atacggagga tacaattcgg cgagctttca gttacgcgtt tcgccccgga
      481 accgagcaat ggccgccagg gtttgttccc aagctccagt tcgtccgggt cgcgcctggg
      541 gtcgaatttg acgtcttggg cgagcgagtc atgccgatcc ggctcgagca tggcaagttg
      601 ccggtccttg gttttcgcgt gggagcgtta gcgtattgca ccgatgtgag ccggattccc
      661 gacgagagct ggccgctctt ggagggatta gacgttctga ttctggatgc gttgcggtat
      721 gaaaagcacc cgacccactt ttccttgagt gaggcactcg cggtcgtcga ggcggtgaag
      781 cctcagcgga ccttcttgac gcatctttcg catggctttg atcatggtcc cacgcaggaa
      841 aagctgccgg atcgtgtggt ccttgcccaa gacgggctgc aactcgcctt ttgagtgcta
      901 ttcgtgttct ctcgagtcga atctcgagag gtgtatcccg ttctcaggag tttggcacga
      961 gatgtctccc gacccgatcc tcgtcgatcg cttgaaacag catggacaaa gtcacctctt
     1021 gaaatggtgg ggggaattga acgaagaaga gcaaggtctg ctggcggctg agatcaggtc
     1081 gattgatttc gagcggctcg accgcttgat cgccgatctg gtggtcagcg accgtgtgac
     1141 ggtgccgccc gcggaccggg tacagccgat cgatgtgatt cgattgcccc agaccgacgg
     1201 cgagcgtgtg gtgcgtcgcc gcgctggggg aatgggtgcc gatgcgctgg ctgccggtga
     1261 ggtgggtgtg attctcgtcg cggggggttc gggcactcgt ctgggctacg aggggccgaa
     1321 gggtacattt ccgatcgggc cggtgtcatc ggcgagcctg ttccagatcc atgccgagaa
     1381 aatcgccgct ctcggcgcgc ggtacggacg gccaatcccc ctctacatca tgaccagtcc
     1441 cgaaaaccac gaagcgacga tcgcgttctt cgagagccat gaccgtttcg gtctggaaca
     1501 tatcaggttt ttcactcagg gccagatgcc ggccgtcgat cgcgcgacca gcaaggttct
     1561 gctggcgtcg cggggtcggg ttgctctctc accggacggc catggcggca cgctggcggc
     1621 cttggccgcc cctgggccgc atgggacgcc gagttgcctg gacgagatgc gcgagcgcgg
     1681 cgtgcgcacg ctcttttact ttcaagtgga caatccgctg gtccggatcg cggaaccgtc
     1741 gttcatcggc ctgcaccggg aagccaatgc cgaggtatcg ttcaaggtcg ttgaacggac
     1801 ctcgcccgaa gaaaaactgg gcgtggtcgt gaccgtcgat ggtcgtcccc aggtgatcga
     1861 atactcggac cttcccgccg agcttgcggg gcgccggctg ccggaggggc cactcgagct
     1921 ctgggccggc agcatcgccg tccatattct cgagcggtcg ttcatcgaac ggctggtcgg
     1981 tgagcacagc cttcctttcc atcgtgcttt gaagaaagtc gcgttcgtgg atgagagtgg
     2041 ccagaacgtg aagccggccg agcccaacgc ggtcaagttt gagcagttta tctttgacgt
     2101 tttgcccatg gcagagcggt gggcgatggt cgagacggac cgagccggtg agttcgaacc
     2161 cttgaagaac gccgtcggcc ccgactcgcc ggccaccgtc catcagcgaa tgagcgatca
     2221 gttcggcaac tggctcgaac aggccggagc cgtcatcccg cgccggccgg acggatcggt
     2281 cccgttcggc atcgagatca gcccgctttt cgccctggat gccaacgatc taaagtcacg
     2341 gatcgaaccg ggtctcgtaa ttgagcgacc gatttactta cggtgacgaa gaagtggact
     2401 gacaatcccg gactgacggg ctgataagcg gctcgccgag ccttaccgat gagagaagga
     2461 gtaagccgag ccttaccaat gagagaaaga gtaagatgaa gactaagatc aggaagagga
     2521 ttaagagaac gagcacgatt aagagtacga tttattttgc cgcgttgcct ttgtcatatt
     2581 cgcgcgttga gttcctcttg atcagcccca tgagcatccg aacaatcctt tgaagtgatc
     2641 gaaccggcat tcgtcaggca cgggcatgtt gtcctaatcc tatatcttga tcctgctctt
     2701 agtcttcttc gtaatcgtag tcttcatctt catcctactc catttttcga tgtaccgccg
     2761 gtcatcctac ccgtcaatca aagattgacg gcgcactaac aacggtccga tttgaccgca
     2821 aacgcaactg caatcttagg gaacaagagc gaggacgacg atgttacacg ccattatcat
     2881 ggccggcggc agtgggaccc gtttttggcc caagagccgg cgcgatcgac cgaagcagtt
     2941 gctctgcctc gctggtgata cgacgatgct ccagcagacc gtggcgcgga tcgaaccttt
     3001 ggtaccgcgc gaccggatca tcgtggtgac tggagcggac caggccgcgg caactcggac
     3061 ccagctcccc gacgtgccag cgcacaacgt gatcgccgaa ccgtccccac gcgatacagc
     3121 accgtgtgtg ggcctcgcgg ccggaatcgt cgcccagaga gacccgaagg ggacgatgat
     3181 cgtcatgccc gccgatcacg tgatcgagcc agcggaatcg ttccgcgcaa cggtcgtggc
     3241 ggcagttgcg gtcatcgatg acgacccgac cgcgcttgtc accttcggca tcaagcccac
     3301 gcaccccgaa acgggttacg gctacatcga gtgcggtgcc ctcttggaga aacgcaacgg
     3361 cattccggtt caccgagtca ttcaatttcg cgagaaaccc gacagagaca cggccgagcg
     3421 gttcctggca gccggtagct tcgcctggaa ctcagggatc ttcgtgtggc gagcccggac
     3481 aatcctggac gagatcgcgg cccatcgtcc gcggcttgcc cagggccttg cgcccatact
     3541 caaatccatc ggaactgccg acgaagcgga aaccctcgcg cgtttatttc ctggactcga
     3601 gcgcgttccg atcgacaagg ccgtcatgga acacgcgcct aacgtgcgcg tgctcgaggt
     3661 cccctaccgc tggaacgacg tcggcgactg gcgtgcactg gctaccctgc tcgaccaaga
     3721 cccggccgga aacgcgaccc agggcaaagt ggtcgtccgg gacacgactg gttccatcat
     3781 catctccgac gatgggggtc tcgtggcgac tctcggcgtc gacgatctcg tcgttgtcca
     3841 ttcgggcaag gccacgctgg tcgcccgcaa agaccagctc gacaagctca aggctctggt
     3901 cgaaggattg ggacaggctg ggtacggatc attcctttga tcggtgttca gctcaattcg
     3961 agattcgccc atccgctccg gcctggtaag atcagatcta ggtcgtgcga gcgtcgaaaa
     4021 tccagctgag ccggacggaa cgccgtcgag cccttggcag cgacggagcc agtcagtcat
     4081 gcgaattatc gcggggcata gacgcggcca caaaatcgat ggtccgcgag cgtcggccag
     4141 gacgcggcct acgagcgacc tggtgcgcga gtcgttattt aacattctgg gcgacctggt
     4201 tgtggaccgt acgg
//
LOCUS       DATAQL010000343         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1560777, whole genome shotgun sequence.
ACCESSION   DATAQL010000343 DATAQL010000000
VERSION     DATAQL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560777"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VHS97_05880"
     CDS             <1..194
                     /locus_tag="VHS97_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX3447761.1"
                     /translation="KITALKGGVPVMVDDQVIGGVGIGGGTGEQDAQVAGAGTQAFLD
                     QLAQSDQKGKATTAVEKPE"
     gene            complement(200..781)
                     /locus_tag="VHS97_05885"
     CDS             complement(200..781)
                     /locus_tag="VHS97_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3447762.1"
                     /translation="MPHTLWRPSLVLALYLNRNAPNARFVQMATVRTDGLPANRSLVF
                     RGFLHDAHQLTFATDTRGRKTAELAAFPRAELCWYFPVTHAQFRIGGTITLVGEDTGD
                     PTLAAARRDTWRALPEATRVSFTWPAPGLPRDSRVPFPTDHPDPLAPLPHFGLMVLDP
                     LDVDHLELNGTPQNRWVYRCDQDGRWSGAEVNP"
     gene            924..1391
                     /locus_tag="VHS97_05890"
     CDS             924..1391
                     /locus_tag="VHS97_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3447763.1"
                     /translation="MSTFPWLSRDGEPSRRAETNRVKAERTRHRVLVVDDNIDAAETL
                     GMLLRLAGQEVVVAHGGVAAIEQALAHVPEIVVLDIGMPDLDGYEVCRRLRQEPSTRN
                     ALLIAVTGWGQDADRTRSHDAGFDHHFVKPVEWSTLEKLLAGLDRQSEPEGEA"
     gene            1388..>1851
                     /locus_tag="VHS97_05895"
     CDS             1388..>1851
                     /locus_tag="VHS97_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447764.1"
                     /translation="MRVTGWHRAIATLVLAAAGLGSATERLARTVAHAQETASPAAAI
                     PHGQDQPPNPPRTALEAARTMTVPPGFQVEVVAAEPDIVNPVAMTFDERGRIWITESL
                     EYPRRRPGPGRDRVKVIEDSDGDGKADRFTVFADGLNIPSGIAVGHGGVWVA"
BASE COUNT          347 a          553 c          643 g          308 t
ORIGIN      
        1 gcaagatcac ggcgctgaaa ggaggggtgc cggtgatggt cgacgaccag gtcatcggtg
       61 gtgtcggcat cggcggcggc accggcgagc aagacgccca ggtcgccggc gcgggtacgc
      121 aggcctttct cgatcaactc gcccagtctg atcaaaaagg taaggcgaca acagccgtcg
      181 agaagcccga ataaggccat tacggattga cttcggcgcc ggaccagcgg ccgtcttggt
      241 cgcaacggta aacccagcgg ttctgcggcg tgccgttcaa ctcgaggtgg tcgacgtcga
      301 gcggatcaag caccatcaag ccgaaatgag gcaagggtgc cagggggtcg ggatgatcgg
      361 tcgggaacgg aacgcgggaa tcgcggggca gccctggcgc cggccacgtg aagctcacgc
      421 gcgtcgcttc cggcagagct cgccaggtgt cgcggcgggc ggctgcgagg gtcgggtcgc
      481 cggtatcctc gccaaccagc gtgatcgtgc caccgatccg gaattgcgcg tgcgtcacgg
      541 gaaagtacca gcaaagctcg gcgcggggaa acgccgccag ttcggccgtc ttgcggccgc
      601 gggtatcagt cgcgaaggtc aactgatgcg cgtcgtgcaa gaacccgcga aatacgagcg
      661 agcgatttgc cgggaggccg tcggtgcgca ccgtagccat ctgtacgaat cgcgcatttg
      721 gcgcattccg gttcaggtaa agcgccagga caagcgacgg tctccacagt gtgtgcggca
      781 tttgcttatt ctggccgcag gaagtcgctc gcgaaagacc aacgagccgg tttcaaccgc
      841 gatcgacgtt tgcagaaacg tgtccatcac gaaacgccgc tggcgtctct ctccgcgcgt
      901 gctctctgcg ccaggtgcgc gggatgagca cgtttccctg gctctctcga gatggcgagc
      961 cgtcgcgaag ggcggagact aaccgcgtga aggctgagcg gactcgacat cgtgtgctcg
     1021 tcgtcgacga caacatcgac gcggcggaga ccctggggat gctcttgcgg ctcgcgggtc
     1081 aagaggttgt cgtcgcgcac ggcggtgtcg cagccatcga gcaggcgctc gcgcatgtgc
     1141 ctgagatcgt ggtgctcgat atcggtatgc ctgacctgga tggatacgag gtctgccgga
     1201 ggctgcgcca agagccgtcg actcggaatg ccttgttgat cgcggtgaca ggctggggcc
     1261 aggatgccga caggacgcgt tctcatgatg caggattcga ccatcatttc gtcaagccgg
     1321 tggaatggag cacgctcgaa aagctgctcg ccggcttgga tcggcaatcc gaaccagagg
     1381 gtgaggcatg agagtaacag gctggcatcg agcgatcgcg acgttggttc tggcggcggc
     1441 cggactggga tcggcgacag agcgtctggc tcgaactgtc gcccatgccc aggaaacggc
     1501 atcgccggcc gcggcgattc cccatggcca ggaccagccc cccaacccgc cgcggacggc
     1561 gctcgaagcc gctcgcacga tgaccgttcc cccggggttt caagtcgagg tggttgccgc
     1621 agagcccgac atcgtcaatc ccgtcgctat gaccttcgac gagcggggac ggatctggat
     1681 caccgagagc ctcgagtatc cccgccggcg accgggaccg ggccgcgatc gggtcaaggt
     1741 gatcgaggat tccgacggtg acggcaaggc cgaccggttt acagtctttg ccgacggtct
     1801 caatatccca tcgggaatcg cggtcggcca cggcggcgtt tgggtggcca a
//
LOCUS       DATAQL010000344         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4919292, whole genome shotgun sequence.
ACCESSION   DATAQL010000344 DATAQL010000000
VERSION     DATAQL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4919292"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2162)
                     /locus_tag="VHS97_05900"
     CDS             complement(<1..2162)
                     /locus_tag="VHS97_05900"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3447765.1"
                     /translation="MAFRFTTLVHWLTDLYLLSTVLLLIGLTVIYRLKQPARRIAVGR
                     SIAAGLGGLAIVASAPSWPRIAAIEWQTSVPALAPQVETQPPVIETAPTKSVEQNAMN
                     GAIALPAELEIVPAPVSAVGRAPKRSLAFAGLPSSRSTLIVVFTIGAAINLVWLALGA
                     IQATLLRRSARTAMPRIQPIVARIFRGHPYSPRVVLSTRIGLPVAMGIVRLMIVLPQR
                     FAETEPDDSLEAALAHEWAHIKNGDLRWLALLRLLNVVLFAHPLFWWLRRTIRADQEV
                     LADAAAATVHGDGRLAYAETLVGWARSCHRPHPGALASAALALWERPSMLHRRVRLLL
                     DRDYRVEQAAPRRWKLAAACTGLLATLLLSMVTLRPSAATAQEMKEAKQVTRNQAADA
                     SRTATSAGDRFEYAGRVLDPDGKPFAGAKLHLAYFGYTGQTSPAIRAMSDSGGHFRIA
                     VRKEDFVDTTQERPWATARVVATAEGFGLGWADAGEWADVHASEAKKIDPLNLTVRLA
                     RDDAPISGRLVDLEGRPVSGVTIRPGEILEPTQGDLSAFIAASTNSRVGWVEIEREYL
                     NRKLWPRESGMPIGIVTDFDGRFSIGGVGAERLLRLAISGPTVQSKEINVLTRSSSPF
                     QVTAARGSRDWGIALYYGANFTHAAAPTKPVFGVVKDKDTGKPLAGVRIACNKTAEFP
                     VYLYNVIETTTDEQGRYRLVGLPKGRGNQVIATPASGQP"
     gene            complement(2176..2628)
                     /locus_tag="VHS97_05905"
     CDS             complement(2176..2628)
                     /locus_tag="VHS97_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3447766.1"
                     /translation="MNPVITALPVGERIDMTDNVPTGRELEALKVLWTRGKATVREIY
                     QEMKPRDGEGELAYTTVLSLLQTMEQKGLVGHESAGKAYVYCPLVQRESTFRKLAGGF
                     LDQVFDGALGEYVARALQSRHPSLKELEELERMIGEAKKKARSSNKSQ"
BASE COUNT          594 a          959 c          961 g          629 t
ORIGIN      
        1 ggctggccgc ttgccggcgt tgcgatgacc tgatttcctc ttcccttggg caacccgacc
       61 aggcgatagc ggccctgttc gtccgtcgtg gtctcgatca cgttataaag atagacagga
      121 aactcggctg ttttgttaca cgcgatccga acgcccgcca aaggcttgcc tgtatcctta
      181 tccttgacga cgccgaagac cggtttggtg ggagcggcgg cgtgcgtgaa atttgctccg
      241 taataaagcg cgattcccca gtcccgacta ccgcgcgcgg ctgtcacctg gaaaggcgac
      301 gatgatcttg tcagcacgtt gatttccttg gattgaactg tcggcccgga gatcgccagc
      361 cggagcaggc gctcggcgcc gacgcctccg atcgaaaatc ggccgtcgaa gtctgtgacg
      421 atcccgatcg gcattcctga ttcgcgcggc caaagcttgc gattgagata ctctcgctcg
      481 atttcgaccc agccgaccct gctgttcgtc gaggccgcga taaaggcaga cagatccccc
      541 tgcgtcggtt cgagaatttc cccagggcga atcgtcacgc cactcaccgg ccggccctcc
      601 agatcgacaa ggcgacccga gatgggcgca tcatctcgcg cgagtcgaac ggtcaggttt
      661 agcggatcga tcttcttcgc ctcgcttgca tggacatcag cccattcgcc ggcatcagcc
      721 cagccgagtc caaaaccttc ggccgtcgcg acaaccctgg ccgtggccca cggacgctcc
      781 tgggtcgtgt caacgaagtc ctctttgcgt actgcgatcc ggaaatgccc gccggagtcg
      841 ctcatcgcgc ggatggcggg cgaggtctgg ccagtgtaac caaaataggc caggtgtaat
      901 tttgcgccgg cgaagggctt gccgtcggga tcgagcacac ggccggcgta ttcaaaacga
      961 tcgccggcgg acgtggctgt gcgcgaggca tcggcggcct ggttccgagt cacttgcttt
     1021 gcctccttca tctcctgcgc cgtcgcggcg gacggccgta gcgtgaccat cgagagcaat
     1081 agtgtggcga gcagacccgt gcaggccgcg gccagcttcc agcgccgggg agcggcctgt
     1141 tcgacgcggt agtcgcgatc aaggagcaga cgcacgcgcc ggtgcagcat cgatggccgc
     1201 tcccaaagtg cgagcgccgc ggacgcgaga gcgcccggat gcggtcgatg gcaagagcgg
     1261 gcccagccga cgagcgtttc agcataggcc aggcggccat cgccgtggac cgtcgcggcc
     1321 gcggcgtcgg cgagcacttc ctggtcggca cggatggtac gtcgcagcca ccagaagagc
     1381 gggtgggcga acagcacgac attgagcagc cggagcaatg ccagccagcg caaatcgccg
     1441 ttcttgatgt gcgcccactc gtgtgcaaga gccgcttcga ggctatcgtc cggctcggtt
     1501 tcggcgaagc gttgaggtaa gacgatcatc agccggacta tgcccatcgc gaccggaagg
     1561 ccgatgcgcg tgctcagtac gacacggggt gaatacggat ggccgcgaaa gattcgtgcc
     1621 acaatcggtt gaatccgggg cattgccgtt cgcgccgaac ggcgcaggag ggtcgcttga
     1681 atcgctccca atgcgagcca gaccaggttg atcgccgcac cgatcgtgaa gacgacgata
     1741 agcgtcgatc gcgatgaagg tagcccggcg aacgcaaggg acctctttgg cgcacgtccg
     1801 acggcactga ccggtgcagg aacgatttct aattcggccg gcagggcgat cgcgccgttc
     1861 atggcgttct gctcgacaga tttggtcggg gccgtctcga tcacgggagg ttgcgtttcc
     1921 acctgaggcg cgagtgccgg gacgcttgtc tgccattcga tggctgcgat acgcggccaa
     1981 ctcggtgcgg acgcgactat ggccaggcca cccaggcctg ctgcgatcga ccgcccgacg
     2041 gcgatccggc gcgccggctg cttgagccgg tagatgacgg tcagcccaat cagcaacaga
     2101 acggttgaca gcaggtacag gtcggtcaac caatgaacga gagtcgtgaa gcggaatgcc
     2161 aacacagctt cctccttact gcgacttgtt cgagcttcgg gcctttttct ttgcctcgcc
     2221 gatcatccgt tccagctctt cgagctcctt cagcgatgga tgccgcgatt gcaacgcccg
     2281 cgccacgtac tcccccaatg caccgtcgaa aacttggtcg agaaacccgc cagcgagttt
     2341 gcgaaaggtg ctctcgcgtt gtaccagggg gcaatagaca taggccttgc cggccgactc
     2401 atggcccact agtccctttt gttccatcgt ctgtagcagg ctcagaacgg ttgtgtaggc
     2461 cagctctcct tctccgtctc gcggtttcat ctcctggtag atctcgcgca cggtggcctt
     2521 gccccgcgtc cagagcacct tgagagcctc gagttcacgc cccgtcggta cgttgtccgt
     2581 catgtcgatc ctctcaccta cgggaagagc cgtaataact gggttcatcg tactagaggt
     2641 tccgtagatg tcaaggacgt ggtttacgaa tgtctatttt tcccgcggac ccgctgttcg
     2701 ttgacgatct ctgtcaataa agaggggtgg gctagcgatg cgtgctcaag tttcttggtc
     2761 cggtatcaga tatccgattg tcgagacgct cttgactctg ggatccacca ccccgatgac
     2821 catgatatat ctgtgctgca tgccttcctg tgaaatcatg tacgccccgg gaaattactg
     2881 tacaaatggt gacaatgtgc cgataattcg tttgaatctg ttgggacggc aatcccggtc
     2941 atgtggccag gaaatcgccg gacttgttta cttgaaagga gacggccatg gcgcacccga
     3001 ttccatctgg gcatcatgcg atcacgccgc acctaatcat caagggcgca gctgaggcga
     3061 tcgagttcta cgagcgggcc ttcggcgcaa cggagctttc ccggatgcca ttcccgggac
     3121 cagacggtca ggtcaagctc ggg
//
LOCUS       DATAQL010000345         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1102818, whole genome shotgun sequence.
ACCESSION   DATAQL010000345 DATAQL010000000
VERSION     DATAQL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102818"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VHS97_05910"
     CDS             <1..309
                     /locus_tag="VHS97_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447767.1"
                     /translation="LIAPIIGDGAAKSSDRFIRLVTKVQDVLGEHQDAVVAAHEIEVA
                     LAEYADHPGFVPAARALLEREHDKAQAARAAFFKVWDKLDRKKSRRWLKIRRTARLGA
                     "
     gene            329..2719
                     /locus_tag="VHS97_05915"
     CDS             329..2719
                     /locus_tag="VHS97_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX3447768.1"
                     /translation="MDHNQFALPVVGQNLQNDQPGTVRDPVCGMNVDPQTAAGSVDHK
                     GRTYHFCSRHCVDKFRADPARYLAPVEAKSGSAPGMDRVTDLPVLAAGTKYTCPMHPE
                     IVRDRPGSCPICGMALEPMTVSADAEVDPELADMSRRLWICAVLTLPLLVLSMAEMVP
                     GLVWPGVLTGRTLVWIQLVLAAPVVVWGGLPFFERGWSSVVHRSLNMFTLIALGTGTA
                     FVYSMMAALFPGIFPASFRDHHGEVGVYFEPAAVIVTLVLLGQVLELRARHQTGGAIR
                     ALLGLAPKTARRLSDDGHEHDVALDQVVPGDKLRVRPGEKIPVDGIVVEGQSTVDESM
                     ISGEPIPVQKVPGEHVIGGTVNGTGGLVIRAERVGSETVLAQIVRMVSEARRTRAPIQ
                     RLADVVSGYFVPLVVLVAVITFICWSLFGPEPRMAYALVNAVAVLIIACPCALGLATP
                     MSIMVGTGRGAGAGVLIKNAEALEILERVDTLVVDKTGTLTEGKPQLVSILPVPGQDE
                     SELLRLAASLEQGSEHPLAAAITTAARNKGLALTAAGAFQSQTGRGATGKVDGRTVAI
                     GSESLLTELGVESVDLVSRSEGLRRDGQTVVFVVIDGRPAGLLGIADPVKESAPAAVE
                     DLRHGGMRVIMLTGDNRTTAEAVARKLKVDEIRAEVLPDQKAAEIKRFQAEGRVVAMA
                     GDGINDAPALAQADVGIAMGTGTDVAMESAGVTLVKGDLRGIVRARRLSRATMSNIRQ
                     NLAFAFLYNALGVPIAAGVLYPWFGILLSPMIASLAMTFSSVSVILNALRLRRMEL"
BASE COUNT          473 a          872 c          926 g          542 t
ORIGIN      
        1 ctgatcgctc cgatcatagg tgatggagcc gccaaaagct ctgatcgctt tatccgtctg
       61 gtgaccaagg tccaggatgt cctcggcgag caccaggatg cggtcgtggc cgcacacgag
      121 atcgaggtag cgctcgccga atacgcggat caccccggat ttgttccggc ggcgcgggcc
      181 ttgctcgagc gggagcacga caaggcccag gccgcgcgcg ccgcgttttt caaggtctgg
      241 gataagctcg atcgcaagaa atcccgccgc tggctcaaga ttcggcggac ggcccgattg
      301 ggggcctgag ccaggggtgg cccgaatcgt ggatcacaat caattcgcgc tgccggtcgt
      361 tggccaaaac ctccagaatg accagccggg aaccgtcaga gaccctgtct gcggaatgaa
      421 cgtcgatccc cagaccgcgg ccggctcggt cgaccataaa ggccgcactt atcatttctg
      481 cagccggcat tgcgtggaca agtttcgggc cgatcccgcc cgctatctgg cccctgtaga
      541 ggccaaaagt ggttcggcgc cgggcatgga tcgagtcaca gatctccctg tcttagccgc
      601 ggggaccaaa tatacttgcc cgatgcaccc ggaaatcgtc cgcgatcgac cgggttcgtg
      661 cccgatctgc gggatggcac tcgagccgat gacggtttcg gcggatgctg aggtcgatcc
      721 cgagctggcc gacatgagcc gccggctgtg gatctgcgcc gtactcacgc tgccgctttt
      781 ggtactttcc atggcggaga tggtgcccgg cttggtttgg cctggagtcc tcaccggccg
      841 aaccttggta tggatccagc tcgtcctggc ggcgcccgtg gtggtatggg gcggccttcc
      901 tttcttcgag cgcggttggt cctctgtcgt ccaccgcagt ctgaatatgt tcacgttgat
      961 cgcactcggg accgggacgg ctttcgttta cagtatgatg gccgcacttt ttcccggtat
     1021 tttccccgcc tcattccgcg atcaccatgg tgaagttggc gtttatttcg agccggcggc
     1081 cgtgatcgtg acccttgttc tcttgggtca ggttcttgag ttgcgtgcac ggcaccagac
     1141 cggcggcgcc atccgcgcgc tgctcggcct ggcccccaag acggcccggc gtttgtccga
     1201 cgacggtcac gaacacgatg tggcgctcga ccaggtcgtc cccggcgaca agctccgagt
     1261 ccggcctggt gaaaagattc cggttgacgg gatcgtcgtc gagggacaga gcacggtcga
     1321 cgagtcgatg atcagtggcg agccgatccc ggttcaaaaa gtgcccggcg aacacgtgat
     1381 tggtggcacc gtcaatggca ccggcggcct tgtcatccgg gccgagcgcg tgggctcgga
     1441 gacggtgctg gcccagatcg tccgcatggt gagcgaggcg cggcgcactc gcgcgccgat
     1501 tcagcgcctg gctgacgtgg tctcgggata ctttgtcccg ctggttgtcc tggtcgccgt
     1561 catcaccttc atctgctgga gccttttcgg ccccgaaccg aggatggcgt acgcgctggt
     1621 caatgcggtg gcggtcttga tcatcgcctg tccctgcgca ctggggctgg ccacgccgat
     1681 gtcgatcatg gtcggcaccg gccgcggggc tggcgcaggt gtcttgatca agaatgccga
     1741 agcactcgag atcctcgagc gcgtcgacac cttggtcgtg gacaagacag gaacgctgac
     1801 cgaaggtaaa ccccagttgg tttccatcct gcccgtcccg ggacaggatg aatccgagct
     1861 gctacgcctg gccgccagcc ttgaacaggg aagcgagcat cccctggccg ctgccatcac
     1921 gactgctgcg cgaaataagg ggctcgcgct cacggccgcc ggagcttttc agtcgcagac
     1981 gggacggggc gcaaccggga aggttgacgg acggaccgtg gcgatcggca gcgagtcgct
     2041 cctcaccgag ctcggagtcg agtccgtcga cctcgtaagt cgatctgaag gcctgaggcg
     2101 cgacggccaa accgtcgtct ttgtcgtgat tgacggccgg ccggcgggcc tgctcggcat
     2161 tgctgacccg gtcaaagagt cggcgcccgc tgccgtcgaa gatttgcgcc acggaggcat
     2221 gcgcgtgatc atgctcacgg gcgataaccg caccacggcc gaggctgtcg cgcgtaagct
     2281 gaaggtcgac gagattcgag ccgaagtcct tcccgatcag aaggccgctg aaatcaagcg
     2341 gttccaggcc gaagggcgag tcgtcgccat ggccggcgac ggcatcaacg acgcgcccgc
     2401 gctcgcccag gccgatgtcg gaatcgcgat gggcacaggc acggacgtcg cgatggagag
     2461 cgccggcgtg actctggtca agggagacct gcgtgggatc gttcgggccc gtaggctcag
     2521 ccgcgcgacc atgagcaaca tccgccagaa tctcgcattc gcgttccttt acaacgcact
     2581 gggcgtgccg atcgcggcgg gagtgctcta cccttggttt ggcatcctgc tcagcccaat
     2641 gattgccagt cttgcgatga cgttcagctc ggtgtcagtg atcttgaatg cgctgcggtt
     2701 gcgcaggatg gagctctgag ccgtcgggcc accttcgaga cgaccgggag ggcgagcttc
     2761 cgatcgagcc gtttttccgt gggcatcgcc gcattgcggc tcggacggag cct
//
LOCUS       DATAQL010000346         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4156316, whole genome shotgun sequence.
ACCESSION   DATAQL010000346 DATAQL010000000
VERSION     DATAQL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4156316"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VHS97_05920"
     CDS             <1..1357
                     /locus_tag="VHS97_05920"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3447769.1"
                     /translation="DSQKSGWLGGGGTVRAGPYVIRRFTDESVADRQRVQSKLPEVAP
                     LVAQAYGRAPFPEVTLEFLNGPAQSTTWPVRRVMSLMGSAGGCKFRLTDPSVSRFHGS
                     LLRTAAGLWIVDLLGQGGITVNDRPVRFGPLADGDALMVGRYQIRIHCRPTRERSTNG
                     SADRDRPRSPARLPRNEQVSSGLTVDEWAAAATSLTQATGEANQPQFPTAIQAVPAFP
                     TTEVMASEPAFPRKFAASELNESMLVPLVNQFGLMQQQMFDQFQQAMAMMVQMFGTMH
                     RDQMEVIRAELDRLHDLTEEFHALKNELAQRTQIHPAETPPLGPAELGQATTTEPRIS
                     PQAPTFERSGVKQPAQVRPPSGIQPPSSTSRVPERQSSSTSPHFTERAPSKPVAAQPP
                     PSERVRAADHRLQAKNDDASPVADSERDSVAWLHQRIMALQQERETRWQKILKLLPGV
                     S"
     gene            1724..3148
                     /locus_tag="VHS97_05925"
     CDS             1724..3148
                     /locus_tag="VHS97_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447770.1"
                     /translation="MGFGKGLAAAKRFGLAGLGFGACAWMVVACALGEGPPRGKQAPP
                     TAPPANAKEGTPTYTKDVASILQTKCQNCHRRHQVGPFALETYEQARKRSRDIAAVTE
                     GRSMPPWKPTAGVGPKLKHDQSLTHAEIAILSAWALAGAPLGDPKDLPPPPKFAEGWK
                     LGPPDLILEPSEGFPVAASGPDVYRCFVLPTNLARDSYLEAVDYAAGEREAVHHLIAY
                     IDTTGLGRQHDADAPGPGYSAPSGPGVEADELSFWTAGSEPHRLPDGVGIRIPAQSDI
                     ILQVHYHPHGKAGVDKTRVGLYFARKPVKQALHWNNASSYNFQLPAGADNVEVKASWF
                     VPVDVEVIAVSPHMHMLGHDMHMSVRLPSGRTENLIEIADWDPSWQSAYYFQKPIPLV
                     AGSVVNVLAHFDNSAHPRNPNQPPKMVKFGPNADDEMCVGYIAVVKKGQDLTAPGARD
                     DLFDIFMRQRDRLLRKQQLAKKPH"
     gene            3333..3944
                     /locus_tag="VHS97_05930"
     CDS             3333..3944
                     /locus_tag="VHS97_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX3447771.1"
                     /translation="MAKSRQSSNPLALAVLVLLFERPMHPYEIAATLRMRNKGGSIKL
                     RYGSLYTVIDSLAQVGLIQAKETVREGKRPERTIYALTPSGVKRMRAWMRELLGTPVK
                     EYPQFEAALSLLPAVPPEEALALLEARVRRLGEMSEELRAAVELAVKHVEALFLVENE
                     YRLSLIDAERQFIEGLIQRIKTDTEYVRTWKAFHSDPARREIS"
     gene            3973..>4505
                     /locus_tag="VHS97_05935"
     CDS             3973..>4505
                     /locus_tag="VHS97_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3447772.1"
                     /translation="MNGRSFHVVIAGGGLGGLCLAQGLKKAGVSVAVYERDRTRTDRL
                     QGYRIHIDPNGSRALYECLPANLFEAFDSTGGVGTRGFNIISHRLEELLHVDLTKVVV
                     ADPIARHRSVSRITLRQVLLDGLDDVVHFDKRLQRYEESRDGQIVAHFDDGSSTIGDI
                     LVGADGGNSKVRAQFLP"
BASE COUNT          911 a         1401 c         1326 g          867 t
ORIGIN      
        1 agactcgcaa aagtcgggct ggctgggggg gggagggacc gttcgcgccg gtccttatgt
       61 aatccgacgt tttaccgacg agagcgtggc tgaccgccag cgagtacaaa gtaagctgcc
      121 agaggttgcg cctctggttg cacaggccta cggccgcgca ccgttcccgg aggtcaccct
      181 cgaatttctt aacggtccgg cacaatcgac cacttggccc gttcggcgcg tgatgtcgtt
      241 gatgggatca gccggcggtt gtaagttcag gcttactgat ccaagtgttt ctcgattcca
      301 tggttcactg ctgcgcaccg cggctggctt gtggattgta gacctgctgg ggcagggggg
      361 catcacggtc aacgataggc cagtgcgctt cggccctctc gccgacggcg acgccctcat
      421 ggtcggccgg tatcaaatcc gtatccattg tcgacctacc cgcgaacggt ccaccaacgg
      481 gtcggccgat cgtgaccgtc caagatctcc cgcccgtcta cctcgaaatg agcaggtttc
      541 gagcggcctc accgtggacg aatgggccgc ggccgcaacg tctttaaccc aagctacggg
      601 tgaggcaaac cagccacaat tcccgacggc catccaagcg gttcccgcgt ttcccacgac
      661 cgaggtcatg gcttccgagc ctgcttttcc ccgcaagttt gccgcgtccg aattgaacga
      721 gtcgatgctg gtcccgctgg tcaatcagtt cgggctcatg caacagcaga tgttcgatca
      781 gtttcagcag gccatggcga tgatggttca aatgttcggt acgatgcatc gcgatcagat
      841 ggaggtgatc cgcgcggaac tcgaccggct tcatgatctg accgaggaat tccatgctct
      901 gaagaatgaa ttagcccagc ggacgcagat acatccggcc gaaacgccac ccctcggacc
      961 cgccgagctt ggtcaggcaa ccacgacaga accacgcatc tcgccccagg cgccgacatt
     1021 cgagaggtcg ggggtaaagc aaccggctca ggtgcgcccg ccatcgggga ttcaaccgcc
     1081 gtcatccaca tcacgagttc cagaacgaca atcgtcgtcg acatcgccgc atttcacgga
     1141 gcgcgcgcct tcaaagccgg ttgctgcgca accccctcct tccgaacgag tccgtgccgc
     1201 cgatcaccgt ttgcaggcca agaacgacga tgcctccccc gtggccgatt ccgaacgaga
     1261 ctctgtcgcc tggctccatc aacggatcat ggcacttcag caagaacgcg agacccgttg
     1321 gcagaagatt ctgaagctct tgcctggggt ctcgtgacca ctctgctcaa ttgaagcgtt
     1381 tctgggcgtt tcttccatcc ccggtcatgt gataaatcga ccatccatgg ccgccggtca
     1441 ctcgaccctt gatggagccc gctgcgactg atatgatcag agaataagca atttttaaaa
     1501 agttttcctg cactaaaccg cgcttgtcgc gattcgtgat agcgcgcttt tgtcccgtct
     1561 cggatgattc aaatcaggac gtcgcagtta caacgatagg aaaaccaaac cggatggcgg
     1621 cggaccggca aaccggctaa aggccggcac ggtttccgat tgatcgacga ccgtttcttc
     1681 ctcttcgacc ggcctttaat cacgttttcg gaggagcgcc ttcatgggtt tcgggaaggg
     1741 gttggctgcc gcgaagaggt tcgggctcgc ggggttggga tttggtgcct gcgcgtggat
     1801 ggtggtcgcg tgtgcgctgg gagaggggcc gccgcgaggg aagcaggcac ctccgaccgc
     1861 gccaccggcg aatgcaaagg aggggactcc gacgtacacc aaagacgtgg cttcgatcct
     1921 ccaaaccaag tgccagaact gccaccgccg ccatcaggtc ggtcctttcg cccttgagac
     1981 gtacgagcag gcccggaagc gttcccgcga tatcgcggcg gtaaccgagg gacggtcgat
     2041 gcctccctgg aagccgaccg cgggagtggg ccccaagctc aagcacgacc agtcgttgac
     2101 ccacgccgag atcgcgatcc tctccgcctg ggcccttgcg ggcgctccgc ttggcgaccc
     2161 taaggacctg ccgccgccgc cgaagttcgc cgagggctgg aagctggggc cgcccgacct
     2221 gatcttggag ccgagcgagg gattccccgt cgctgcgtcg ggccccgacg tctaccgctg
     2281 ctttgtcttg cctaccaatc tggcgcgtga ttcgtacctg gaagcggtcg attacgccgc
     2341 gggcgaacgt gaggccgtgc atcacttgat cgcctacatc gacacgaccg gactcgggcg
     2401 acagcatgac gcggacgcac cgggcccggg ctactcagcc ccatccgggc cgggcgtcga
     2461 agccgacgaa ttgagctttt ggacagccgg tagcgagcca caccgcttgc ccgatggagt
     2521 cggcatccgc attcccgcgc agtctgatat catccttcag gtccactacc acccccacgg
     2581 caaggccggc gtcgacaaga cgcgagtggg cctctatttc gcgcgcaaac cggtcaaaca
     2641 ggcgttgcac tggaacaacg catcgagcta caacttccaa ctgcccgccg gggccgacaa
     2701 cgtcgaagtc aaagcgagtt ggtttgtccc cgtggacgtc gaggtcatag ccgtctcccc
     2761 ccacatgcac atgctcggtc acgacatgca tatgtccgtt cgcttgccca gcggccgcac
     2821 cgagaacctg atcgagatcg ccgattggga cccctcctgg cagagcgcct actattttca
     2881 gaagccgatc ccgttggtcg cgggctcagt cgtcaacgtc ctcgcacatt tcgacaactc
     2941 cgcccatcca cgcaacccca accagcctcc caagatggtc aaattcggcc ccaacgccga
     3001 cgacgagatg tgcgtcggct acatcgccgt cgtgaaaaag ggtcaggacc tgacagcgcc
     3061 cggagctcgc gacgacctgt tcgacatctt catgaggcag cgagaccggc tccttcgcaa
     3121 gcagcagttg gccaagaagc cacactgagc tcagcgaatt cggcgcgcaa cctttcgtat
     3181 cgagtcagcg aacgccgctg agagttcgcg ccgtttattc accggcgtcc ccactcccga
     3241 acaatattcc ggtccaccac ccgaataatc ttgcgacaga tatccgtcgt tgatatatac
     3301 gcagacggat attctaagtc ggaggtgggt ggatggcaaa gagcaggcag tcatcgaatc
     3361 cgctggcgct ggcggtgctc gtgttgctat tcgaacggcc aatgcatccg tacgaaatcg
     3421 cagcgacgct caggatgcgt aacaagggag gaagcatcaa gttgcgttat ggctccctct
     3481 ataccgtgat cgattcactc gcccaagtcg gcctgattca agccaaggag acggttcgtg
     3541 aaggcaagcg gccggaacgg acaatctatg cgttaacgcc gagtggagtc aagcggatgc
     3601 gtgcgtggat gcgtgaactc ttaggtacgc cagtcaagga gtatccgcaa ttcgaggctg
     3661 cgctgtcgct cttaccggcc gtgccgccgg aggaggcgct cgctcttctg gaggcgcgcg
     3721 tacgtcggct cggcgaaatg agcgaagagc ttcgggcagc ggtcgagctt gcggtcaaac
     3781 acgtcgaagc gttgttcctg gtcgagaacg aataccggct cagtctcatc gatgccgagc
     3841 ggcagtttat cgaaggactc attcagcgta tcaagaccga caccgagtac gttcggacct
     3901 ggaaggcgtt tcactccgat ccagcacgga gggaaatctc gtgacctcaa aaacctgagg
     3961 agagtatttg tcatgaatgg tcgatcgttt catgtggtca tcgcgggtgg gggtctcggg
     4021 gggctttgcc tggctcaagg gctgaaaaag gcaggggtca gcgtggcggt ctatgagcgc
     4081 gaccgaacac gcacagaccg cctacagggc taccggattc acatcgaccc caatggcagc
     4141 cgggctcttt acgaatgcct gccagccaat cttttcgagg ctttcgactc aaccggtggg
     4201 gtcgggaccc gaggattcaa catcattagc caccgtttgg aggagctctt gcacgtcgat
     4261 ctgaccaagg tcgtcgttgc cgacccgatc gcgcgccacc gatccgtaag ccgcatcacg
     4321 ttgcggcagg tcctgcttga tgggctcgac gacgttgttc acttcgacaa gagactccag
     4381 cgatacgaag agtcccgcga cggccagatc gtcgcccact tcgatgacgg cagctcgacc
     4441 atcggtgata tcctggtcgg tgccgacggc ggcaattcca aggtccgcgc ccagttcctc
     4501 ccgca
//
LOCUS       DATAQL010000347         8121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1561156, whole genome shotgun sequence.
ACCESSION   DATAQL010000347 DATAQL010000000
VERSION     DATAQL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561156"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3593)
                     /locus_tag="VHS97_05940"
     CDS             complement(<1..3593)
                     /locus_tag="VHS97_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3447773.1"
                     /translation="MGLLQRSTVRSRALAVVFGVLLVVIVSVSAAWPIVAADDQSSLG
                     AGVYPGAYAITGAKIVTAPGKSIDSGTIVIRRGIIEAVGPAKDIEVPYDAEPIDGKGL
                     VVYPGFIDLYTTIGQHGGAERSATGKGRPVDLAESPLISTPPDNRKGLTPEFDVASAL
                     ELTEALAEPRRRLGFTDLVSAPSGSIATGQSALVSLSGLPRREAIVAAPVALHVHVAP
                     PSEPAASGAPATPALPGPPGLVPGQTPGPGPGPGRRRGLVQEGASENPYPRVLMGSVA
                     HFRQAMLDADHFQKLNVYYEAKGGSRPPFDPALKALWAARTKKIPVWWEGNSRDEIHR
                     ALDLANEFGTTAVIVGGREAPQVAERLKAGQVPVVLRLNFPEEPRVPTEEEYRKKTAT
                     DRDEPLRLLAHRKTKWKEQVGAAAALAKAGVSFAFSTEGIDRLDTFPTVVRQLITAGL
                     TADLALAGLTQNAAIIAGVDRRLGTLEPGKLGHVTVMTAPFNDEKAKVKYVLIDGLKF
                     EVKPEDRSRAKSRVGGPDGLASAGEPGERPRFGGRRGASPDDDETPKPAERKDSPDRP
                     DQKTKDEPPKKTADDKNPGTAKEESADAQSKKEPDATTKPSDAKASDAKKPESQSKSS
                     EATKTADSKSDAPKPSAAGADAAKPPATPFVDVATEFDDDRKPALHTGGDVLIKGATI
                     LTVTKGTIAKGSILVEHGKIKAVGTGLTAPEGVKVIEASGLVAMPGIIDTHSHIAIQG
                     GVNEGTLSVVPEVRVKDVVTGEDVAIYRALAGGTTTARLLHGSANTIGGQDAVVKLRY
                     GKPGRDMIIREGPQGVKFALGENVTRSRGRFPNTRMGVESVIEKAFDEARAYRAAWDH
                     YKKTVQAKGPDQGGPPPRVDLRLEALAGVLDGSIKIHSHCYRSDEILMLLRTAQKYGV
                     RVQSLQHVLEGYKVAAEIAAHGASASTFSDWWAYKIEAYDAIPHNAALLTEAGASVCI
                     KSDDPELMRHLNLEAAKMVKYGAVSESQALAMITINPARELGLDGRLGSIEVGKDADI
                     ALFNGHPFDAFSRCELSLIDGEVYFQRREQDGGFGVRPGEHTAMPAAAESVRNRSIEI
                     AAQPKNLFALIGANLHPVSGPEIKGGTLVIAGGKIAAIGATDTPIPPEAQTIEMAGLD
                     IWPGMVDAGSTVGLYEIGSLNETQDAADAAQFQPELRSSTALHPDSEHIPVT"
     gene            3976..4719
                     /locus_tag="VHS97_05945"
     CDS             3976..4719
                     /locus_tag="VHS97_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447774.1"
                     /translation="MRQYFGAILTMTLSFVAFGPARADDLDAKSILDKAIKALGGEEK
                     LAKVAAYTWKSEGTITFNGNENEFKSEVTVKGLDHLRREFGNDNFNFVVVLAGNKGWR
                     KGGDNFTELEGDALANEKRGVYLQVIPITIVPLKEKGFKCETGGEEKVDGKPAVVLKI
                     TGPDGKDFTLYFDKESGLPVKQVAKVLGFQGQEYTAETTFADYKDFDGIKKATKIEVK
                     RDGEAFQKFNVKEFKVLDKVEAATFTEPK"
     gene            4820..5602
                     /locus_tag="VHS97_05950"
     CDS             4820..5602
                     /locus_tag="VHS97_05950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX3447775.1"
                     /translation="MGFSLMQIVDLSATIAPSAPDASPLQRVDIHYTSHAQGAAQIQA
                     MLHVPGHLLRNGEGWAIEELGRLGTHNVTHVDAPWHYNSQIQGRKAATIDELPLEWFF
                     GDAVVLDMTFKADGEKVDVADVQSALEKIGYALKPRDIVLVRTGRDAFYGQPDYMFRG
                     CAVTVQATRWLYDRGVRVMGIDAWGWDGPLDRQAKEALTRQEPGVFWAAHQADLAYSQ
                     IERLVNLSSLPPFGYKVACFPLKIQGGSAGPARVVAILDDPD"
     gene            5726..7102
                     /locus_tag="VHS97_05955"
     CDS             5726..7102
                     /locus_tag="VHS97_05955"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3447776.1"
                     /translation="MNRSQGNSSRRAFLGRTARASAAAFAFPTIIPGSALGLSGRPAP
                     SNRITLAVIGTGNQGFNDIKSFLRDDRVQIVAVCDVNRESAGYWDGKVGGREPARRLV
                     DDHYAGKGSSEAHHVCELSGDFREILGRHDIDSVEVCTPDHWHALMVVEACRAKKDIY
                     CQKPLSLTVAEGRAMSYAVNKSKVVFQTGSQQRSDWRFHRACELVRNGRIGELRTVRI
                     GMPKGRTDYAKTEAHKKPEAVPQGFDYNTWLGPAPDVPYAPARCHVNFRWIYDYSGGQ
                     VTDWGGHHPDCAQWGMGTELTGPLEIRRAKGVFPPDELWNTATEYSFEAVYENGVTMI
                     ISSKEKMGVTFEGTEGKIYVDRGKLEADPKSLLDSQIGPVGIHLYKSDDHFRNFIDCV
                     ISRQPTAAPVEVAHRSITICHLGNIAMRLGRERLRWDPRAEQIIGDDEAAKMLSRPYR
                     APWALPAI"
     gene            complement(7155..7604)
                     /locus_tag="VHS97_05960"
     CDS             complement(7155..7604)
                     /locus_tag="VHS97_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447777.1"
                     /translation="MANNQAVLVHPNLFQLSGRHHHPGNNLQVSYLTHSGPATPQFPQ
                     GPPHFTYQDESQSLSFVGPEVEVVNSELGDVVSVVIRRTIDSGSTTFSLLVPNVNIVA
                     GQQAVIHTEGITAIHRLSLAPGLDQGQRDQYSVTPLEGTASNIIVPL"
     gene            complement(7800..>8121)
                     /locus_tag="VHS97_05965"
     CDS             complement(7800..>8121)
                     /locus_tag="VHS97_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447778.1"
                     /translation="RRSNSPYRLHWKNLCSCRSSNRCSQPHLLALGTTGVELDADEIE
                     LARRLAKTVNGVMTVEKTKKAAAAPEAAGQTDRARRLRSWLTEVTAGKEKLKGRAAGS
                     YPGG"
BASE COUNT         1518 a         2480 c         2528 g         1595 t
ORIGIN      
        1 gtgaccggga tgtgctcgct gtcggggtga agggcggtgc tgctgcgcag ctcgggctga
       61 aactgggcgg cgtcggccgc gtcttgggtc tcgttaaggc tgccgatctc gtacagaccg
      121 accgtcgaac ccgcatcgac catgcccggc cagatatcca gtccggccat ctcgatggtc
      181 tgggcttcgg gcgggatcgg ggtatccgtg gctccaatcg ccgcgatctt gccgccggcg
      241 atcacgagcg ttcccccctt gatttccggt cctgagaccg ggtgtaggtt ggcgcctatc
      301 agggcgaaaa ggtttttggg ctgcgcggcg atctcgatcg agcgattccg caccgactcg
      361 gccgcggccg gcatcgccgt gtgctcaccc ggccggacgc cgaatccacc atcttgctca
      421 cgccgctgga aatacacctc gccgtcgatc aagctcagct cgcatcgtga aaacgcgtcg
      481 aaggggtggc cgttaaagag cgcgatgtcg gcgtccttgc ccacctcgat cgagcccaag
      541 cgaccatcca gaccaagctc gcgtgccgga ttgatcgtga tcatcgccag cgcctggctt
      601 tcggagaccg ccccatactt gaccatcttg gcggcctcga ggttgagatg gcgcatcagc
      661 tcaggatcat cgctcttgat acacacactg gcgccggcct cggtcaagag cgcggcattg
      721 tggggaatcg cgtcgtaagc ttcgattttg tacgcccacc agtccgagaa agtagacgcg
      781 ctggcgccgt gggcggcgat ctcggcggcc accttgtaac cctccagcac gtgctgcagc
      841 gactgcacgc gcactccgta cttttgagcc gtgcgcaaaa gcatcaggat ctcgtcgctg
      901 cgatagcaat ggctatggat cttgatcgag ccgtccaaga cccccgcaag cgcttcaagc
      961 cggaggtcga ccctcggcgg cgggccccct tggtcggggc ctttggcttg caccgtcttc
     1021 ttgtaatggt cccatgcggc acgatacgcc ctggcctcgt cgaaggcctt ttcaatcacc
     1081 gactcgactc ccatacgggt gttgggaaat cgtcctcgcg accgggtcac gttttcgccg
     1141 agcgcaaatt tcaccccttg agggccttcg cggatgatca tgtcgcggcc cggcttgccg
     1201 taccgcagct tgacgacggc gtcctggcca ccgatcgtgt tggccgaccc gtgtagtaag
     1261 cgggccgtcg tcgttccgcc cgcaagggcg cgatagatcg cgacgtcctc gcccgtgacg
     1321 acgtctttga ctcgaacctc gggcacaacc gacagcgtgc cctcattgac gcctccttgg
     1381 attgcgatgt gcgagtgtgt gtcgatgatc ccgggcatag ccaccaggcc cgacgcctca
     1441 atcaccttga cgccttccgg cgccgtcaaa ccggtgccga cggccttgat cttgccgtgt
     1501 tcgaccagga tcgaaccctt ggcgatcgtc cccttcgtca ctgtcagaat cgtcgcgccc
     1561 ttgatcaaca cgtcaccgcc ggtgtgcagc gccggcttgc ggtcatcgtc aaactcggtc
     1621 gcgacgtcga cgaacggagt cgctggcggc ttggcagcat cggctccggc ggcgctcggt
     1681 ttgggcgcat cggatttcga atccgcggtc ttggtagcct cgctcgattt cgattgcgac
     1741 tcaggttttt tggcgtccga agctttcgcg tcgctaggct tcgttgtcgc gtccggttct
     1801 ttcttggact gggcgtccgc ggattcttcc ttggcggtgc caggattctt gtcgtcggct
     1861 gtcttctttg ggggctcgtc cttggtcttc tgatcgggac ggtccggaga gtccttgcgc
     1921 tcggccggct tgggagtttc gtcgtcgtcc gggctggcac cgcggcggcc gccaaaacgt
     1981 ggccgctcgc ctggttcgcc ggcggaagcc aggccgtcgg gtccgccaac ccgtgacttc
     2041 gcgcggctac ggtcctcggg cttgacctcg aatttcaggc cgtcgatcaa cacatatttg
     2101 accttggcct tctcatcgtt gaacggggcc gtcatgacgg tcacgtggcc gagcttgccg
     2161 ggctcgagcg ttccgagtcg gcgatcgacc cccgcaatga tcgcggcatt ttgggtgagg
     2221 ccggccaagg caagatcggc ggtcaatccc gccgtgatca actggcggac gacggtcggg
     2281 aaggtgtcga gccgatcgat tccctcggtg gaaaaagcga acgagacgcc cgccttggcg
     2341 agcgccgcgg ccgcgccgac ctgctctttc cacttggtct tgcggtgggc caagagtcgc
     2401 aggggctcat cgcggtcggt agcggtcttc ttgcgatatt cctcctcggt cggcaccctg
     2461 ggctcttcgg ggaaattgag ccgaaggacg accggaacct gcccggcctt gagccgctcg
     2521 gccacctggg gggcctctcg gccgccgacg atgacggcgg tcgttccaaa ctcgttggcg
     2581 aggtcaagcg cgcggtggat ctcgtcgcgg ctgttgccct cccaccacac cgggatcttc
     2641 ttggtccgcg cggcccagag tgccttcaaa gcgggatcaa acggaggccg gctgcccccc
     2701 ttggcctcgt agtaaacgtt gagcttttgg aaatgatcgg cgtcgagcat ggcctggcgg
     2761 aagtgagcga ctgagcccat caagacgcgg ggatacgggt tttcgctggc tccttcctga
     2821 actaaacctc gccgccggcc cggtccaggg ccggggcccg gagtctgacc cggaacgagg
     2881 ccgggtggac ccggcaatgc gggagttgcg ggtgcgccgc tggctgccgg ctccgagggg
     2941 ggcgccacgt gcacgtgcag cgcgaccggc gcggccacga tcgcttcccg gcggggcaag
     3001 ccgctcaggc tcacaagcgc gctctggccg gtcgcgatcg acccggacgg ggccgacacc
     3061 aagtcggtaa atcctagccg ccgccgcggt tcagccagcg cttcggtcag ctcgagagcg
     3121 ctcgcgacgt caaattctgg cgtcaagcct ttgcggttgt ccggcggtgt cgagatcaag
     3181 ggcgactcgg ccaggtcaac tggccgcccc ttaccggtcg ccgatcgctc ggcgccgccg
     3241 tgctgaccga tcgtggtgta caggtcaata aaccccggat agacgaccag gcctttgccg
     3301 tcgatcggct cggcatcata ggggacctcg atgtccttgg ccggcccgac cgcctcgatg
     3361 atcccgcgcc ggatcacgat cgttcctgaa tcaatcgact tgccgggagc cgtcacgatc
     3421 ttggccccgg taatcgcgta agcacccggg tagaccccgg cccccaggct cgactggtcg
     3481 tcggccgcga cgatcggcca ggcggcagac acgctcacga tcacgactaa cagcactccg
     3541 aacacgacgg caagcgctcg cgagcgcaca gtcgaccgct gtaagagccc catgatgttt
     3601 cttcctcggt tgtcggttat ctcgaagggg ttgttcagag tgttgttgtc gttcgcgaca
     3661 agcgcgccct tccggcccag gaacacagcg caggggagag aagaggtgat cccaatgcgg
     3721 ctcctgcggt ttcatttttc gagatcggga tgattataaa agcgtcgtcc ccagaaagca
     3781 cgccccaaga ttcgggaaag gatgattcgc caacaccttt cacttcggcc ggagtggcat
     3841 atcctaggag gggtttcctg agcgctttca ggaataacca actcaggagc tggacgcgat
     3901 ccaatggcca gtcaagaaaa agacacctgc gattcgtgtc tagctttccc cattttcccc
     3961 acgtgaggtg agctgatgcg gcaatacttc ggcgcgattc tcacaatgac cctttcgttc
     4021 gtcgcgttcg ggccagcccg ggccgacgac ctagacgcga agtcgatcct cgacaaagcc
     4081 atcaaggctt tgggcgggga ggaaaaactt gccaaggtcg cggcatacac ttggaaatct
     4141 gagggcacca tcactttcaa tggaaatgag aacgagttca agagcgaggt gaccgtcaaa
     4201 ggcctcgacc acctccggcg cgaattcggc aacgacaatt tcaatttcgt ggtcgtcctc
     4261 gctggcaaca aaggctggcg caaggggggc gacaacttca ccgagctgga aggcgacgca
     4321 ctcgccaacg agaagcgcgg cgtctacctc caggtcatac cgatcacgat cgtgcctctg
     4381 aaggaaaagg gattcaaatg cgagaccggt ggtgaagaaa aggtcgatgg caagccggct
     4441 gtcgtcctta agatcaccgg gccggacggc aaggacttca cgctctattt cgacaaagaa
     4501 agcggccttc cggtcaaaca ggttgccaaa gtcctcggtt tccagggcca ggaatatacg
     4561 gcggagacaa ccttcgccga ctacaaggat tttgacggca tcaagaaggc caccaagatc
     4621 gaggtcaagc gtgacggcga ggcgttccaa aagttcaacg tcaaggagtt caaagtcctg
     4681 gacaaggtag aggccgcaac cttcaccgaa cccaagtgat gacgactaat cggatgccag
     4741 cggttgcaag gttgtaatta tgatctccgc tcacccctga cgactcgcac gtggcggacg
     4801 ggcgcgagtc gtcaggtaca tgggattcag cctgatgcag atcgtcgacc tttcggcaac
     4861 aatcgcgccg agcgcgcctg acgcgtcgcc actacagcgc gtggatatcc actacaccag
     4921 ccacgcccag ggggccgcgc agatccaggc catgcttcat gtccctggtc acctgctcag
     4981 aaacggcgag ggttgggcga tcgaggaact tggcaggctg ggtacccaca acgtcaccca
     5041 cgtcgatgct ccctggcact ataacagcca gatccagggg cggaaggctg ccacaatcga
     5101 cgaactgccg ctcgagtggt tctttggcga tgcggtggtc ctcgacatga ccttcaaggc
     5161 cgatggtgag aaggtggatg tcgccgacgt ccaatctgct ctggagaaaa tcggctatgc
     5221 cctcaagccg cgcgacatcg tgctcgtgcg aaccggccgt gacgcgtttt acggtcagcc
     5281 tgactatatg ttccggggct gtgcggtgac ggtccaagcc actcggtggc tgtatgaccg
     5341 cggagtgcgt gtgatgggga tcgacgcctg gggttgggac ggcccgctcg atcggcaggc
     5401 gaaagaagca ctgacccgcc aggagccagg cgtcttctgg gcagcccatc aagcggacct
     5461 ggcctactcg cagatcgagc ggctcgtcaa tttgagttct ctgccccctt tcggctacaa
     5521 ggtggcgtgt tttccgctca agatccaggg aggcagcgcg ggaccggcgc gtgtcgtggc
     5581 gatcttggac gatccggatt gagtggattg aatcaacgtg ggcgatcaag cgatcgtccg
     5641 ggacaatagg ggccaatgaa atgtcggcgc agggccgtgg tcattctctc ctaaacccat
     5701 gcggtcagca tttgagaaaa ctacgatgaa ccgatcccag ggcaactcgt ctcgccgcgc
     5761 ttttcttggt cggaccgcca gagcatcggc ggccgccttc gccttcccga cgattatccc
     5821 aggttccgcc ctcgggctct cggggcggcc ggcacccagc aatcggatca ccctcgcggt
     5881 gatcggcacg ggcaaccagg gttttaacga catcaagtcg tttctccgcg acgaccgcgt
     5941 gcagattgtg gccgtgtgcg acgtcaaccg cgaaagcgcc ggctactggg atggtaaggt
     6001 cggcggccgc gagccggctc ggcgacttgt cgacgaccat tatgccggga aggggtccag
     6061 tgaggcgcac cacgtgtgcg agttgagcgg cgactttcgc gaaattttgg gcaggcacga
     6121 tatcgactct gtcgaagttt gtactcccga tcactggcac gcgcttatgg tcgtcgaggc
     6181 atgcagggcc aagaaagaca tctattgcca gaaacccctc tcgcttaccg tcgctgaggg
     6241 tcgcgcgatg agctacgcgg tcaataagtc gaaggtcgtc ttccagaccg ggagccagca
     6301 gcgctcggat tggcggtttc accgcgcctg cgaattggtt cgcaacggcc gtattggcga
     6361 attgcgcacc gtccgcattg gtatgcctaa gggccggacc gattacgcca agacggaagc
     6421 acacaagaaa cccgaagcgg tcccccaagg cttcgactac aacacgtggc tgggtcccgc
     6481 ccccgacgta ccctacgccc ccgcgcgctg tcatgtcaac ttccggtgga tctacgacta
     6541 ctccggcggc caggtcaccg actggggggg ccaccatccc gactgtgccc agtggggcat
     6601 ggggaccgag ctaaccggcc cgctcgagat cagaagggcc aagggggtct ttcctcccga
     6661 cgagctctgg aacacggcca ccgagtattc gtttgaggct gtttacgaaa acggagtgac
     6721 catgatcatc tccagtaaag agaagatggg ggtcaccttc gagggaacgg aaggcaagat
     6781 ttatgtcgac cggggcaagc tggaggccga tcccaaatcg ctgctcgatt cccagatcgg
     6841 ccctgtcggc atccaccttt acaagagcga cgaccacttt cgcaatttca tcgattgcgt
     6901 gatctcccgg caaccgacgg cggcgccggt cgaggtcgca caccgatcga tcacgatctg
     6961 ccacctggga aatatcgcca tgagattggg tcgcgaacgg ctgcgctggg acccgcgggc
     7021 cgagcagatc atcggcgacg acgaagcagc caagatgcta agccggccct atcgcgcgcc
     7081 ctgggcgctt ccagcgatct aatacgagtg ccgcggcatc ggtgctagtg aagcgcggga
     7141 atgtatctga ctcctcagag tgggacgatg atatttgagg cggttccctc caacggcgta
     7201 acggaatact ggtccctctg cccctgatcc aagccaggcg ctaaggatag tcggtggatg
     7261 gccgtgatcc cctcggtgtg aatcacggct tgctggccag cgacgatatt gacattgggg
     7321 accagcagac tgaacgtggt tgagcccgag tcgatcgtgc ggcgaatgac cacgctgacg
     7381 acatctccga gctctgaatt aaccacctca acttccggcc cgacaaatga gagtgattgg
     7441 ctctcatctt gataagtaaa atgaggtggc ccttgcggga actgaggagt cgccgggcca
     7501 ctgtgggtga gatagctcac ctggagattg ttaccggggt ggtggtgccg accactgagc
     7561 tggaaaaggt tggggtggac gagtacggcc tgattgttcg ccatggcgaa ttctcctgat
     7621 ggaagttgtc cgaacgctct ggttgtctcg tggaaggctc ggtctgacgc tcgagacgtc
     7681 cccgacgtcc tccaccggat ctatcaagag ttggcacaaa aaagcgcaaa gtcatttgcc
     7741 cttctttcac cctacctgac atcgaaataa ctgcatccta ggtttgctat gatcgtgaac
     7801 taacccccgg gatagcttcc agcggctcgg cctttgagtt tttccttgcc ggcagtgact
     7861 tccgtcagcc aagatcggag gcgcctggca cggtcggtct ggccagctgc ttcgggcgcc
     7921 gctgcggcct tctttgtctt ttcgacggtc ataacaccat tgacggtttt cgcgaggcga
     7981 cgggccagtt cgatctcgtc ggcgtcgagc tcgactccgg tcgttccgag ggcgagcaga
     8041 tgaggttgcg aacatcggtt tgaactccgg cacgagcaga ggttcttcca gtggagacgg
     8101 taaggggagt tggagcgccg a
//
LOCUS       DATAQL010000348         6879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3241522, whole genome shotgun sequence.
ACCESSION   DATAQL010000348 DATAQL010000000
VERSION     DATAQL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241522"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VHS97_05970"
     CDS             <1..805
                     /locus_tag="VHS97_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX3447779.1"
                     /translation="AGPDLLMKLEIELVEAARGTTRAIDVTRQDFCAECRGSGARKGT
                     VATTCNYCGGRGQIVQARGFFQVATTCPACGGEGVRITDPCPSCRGGGRVPSTAHLQV
                     DIPPGVESGMWLQLRGQGELGDVGAPRGHLRIQVLVKKHPFFERQRNDLVCQVPISFA
                     QAALGAMVEVPTLDGREHLEVPRGSQSGDVLRIKGRGMPDIGGRARGDELVEIVVETP
                     RHLTTRQEELLREFAEIEHHQVSPRRRSFFEKLRDYFTEDADPDDSNDS"
     gene            869..1471
                     /gene="grpE"
                     /locus_tag="VHS97_05975"
     CDS             869..1471
                     /gene="grpE"
                     /locus_tag="VHS97_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX3447780.1"
                     /translation="MNEKAQKPNDVDASGAEFPENRGPDRETGPGPTEGQPENGETMS
                     VSEQIARLKHERDELRDQALRARAEFANYQKRAKQQAEAERAYAVGSLAKDLLDPLDN
                     LDRAIEALRAKGAEGITSGLDMVLKQLLEIMAKHGVQPISAHGHPFDPNMHDAILQQP
                     SLEHPEGTVVAELSKGYTIADRVLRPSKVAVSVKPTPKGE"
     gene            1476..1790
                     /locus_tag="VHS97_05980"
     CDS             1476..1790
                     /locus_tag="VHS97_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX3447781.1"
                     /translation="MPTYDYICDSCGHEFEAYESIKAEPLTDCPECKIPQLRRKIGPG
                     AAILFKGSGFYQTDYRSESYKKAEKAEKASNEPAKPASDGASSTKSEPAKAAPAPTKS
                     EP"
     gene            1787..1993
                     /gene="yacG"
                     /locus_tag="VHS97_05985"
     CDS             1787..1993
                     /gene="yacG"
                     /locus_tag="VHS97_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX3447782.1"
                     /translation="MIRGRCPICSKSYEIASVDALPSFPFCTDRCRMIDLGRWIDGKY
                     AIPGSAKDQKQQEEGDESSADDVE"
     gene            complement(2046..3536)
                     /locus_tag="VHS97_05990"
     CDS             complement(2046..3536)
                     /locus_tag="VHS97_05990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX3447783.1"
                     /translation="MAQQDRPQLHTLLIFYAVLALAWLAFARWVVPPLLIAEHSPGIL
                     AYLKRFIELPPALFLTRDILGRWREYSGAVLLAIFLHFSVFFIVRRYDLRAAASRPTT
                     VVQAENRTSLALLIVSFVFLGVTALSGPRHDYYFYRQMWYEIAQGHDPWFKVYGAGGH
                     VPLNAYGPLFNLLTGLSWVNPYAPKLMCAYSYVIFAISQIKGFTASRAPSVIQLIMLF
                     ALFWNPFPWVEIAARGHFDILVGLLCLGAVRAWTRGYDIRSGACLALGVLLKYLPIVL
                     LPALAVDRGRVRWRFLVSALSTIAFGMELSCLVWGISTLSPLELAANRDATALSIYYF
                     VRGRYSPLLLFGAMTNLDYLAPYVLFSALLLAWRWAWVRQPDALAAAVVVAITTVLFY
                     RTAYPQYQMVPFVLGSAWAVRHWDSITCRTWRVLAIACYFGWLAAFDLYYAFDDEGPG
                     SRYWSTVQYVVGLPSFLVGCAFLAGVIRSATTDDKASIQPPESKPR"
     gene            3724..5463
                     /locus_tag="VHS97_05995"
     CDS             3724..5463
                     /locus_tag="VHS97_05995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3447784.1"
                     /translation="MAVSPALPEVGQVVRVGFEGGPTPPKLREDGARARPPRASTMAR
                     LFPRGERIDRLVLAFVALGVVLRVGHYLSNYPLWGDEAFLAISFLRRGYLDLLLPLEY
                     GQICPILFLWAELTAVKLFGFSEMSLRLCPLICGVGSVFLFRHVAGRVLNGPAVLMAV
                     AIFAVSIHPIRHSADVKPYASDLLVALGLLALAIEWCVSPQRTGWLWLLVGFAPFALA
                     ASYPAVFVAGGSALALAASVWKTRRRGPRVALAAYVLVVATTFAALFIGYVHGQRGAS
                     LPAIQTYWADSFPPLDSAIRLASWLVTTHAGNMFAYPVGGGLGASSLTLILVLIAAIV
                     LWRRGHGTILALLVMPMGVALSAAALKLYPYGGEARIMQYIAPAVCLLTGLASSTLLG
                     LLPRPTARAAAIRVAVITLAVMGIVALVNDFRHPYRSIYDHQAREFARRFWPEQARGA
                     ELACLQWDLGICQRGAAVARTAIYLCNQHIYSPKRRRGLGPRWALVTLDRPLRCVSFD
                     AVHLKGPKATAWLESMRANYNFRGQLDLVVPITRLDMKPGNDHVFVFEFQPKSDRPIE
                     RVVTGVATDRTVR"
     gene            complement(5577..6800)
                     /locus_tag="VHS97_06000"
     CDS             complement(5577..6800)
                     /locus_tag="VHS97_06000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3447785.1"
                     /translation="MDSFQNDHGNEGSRRNFLKTAAAVGGTAAIGSADSAWAQPAAST
                     IPKVTLGKTGEKIPVLGMGTSWEVAPSFVQAALHAGVRYIDTSEAYESGNVEKTLGEV
                     LERTKMRKEVYLVTKNSGGKVGGSRAFPTFEKHLTASMERLRTDYIDCYYLHGVTGRE
                     IPLLKDPDVKAAFEKLKKAGKIKFCGLSCHDGRLPEIVTAAAAAGWMDQIMIQYNYRT
                     MTTDAVKKALDAASKANLAIVAMKTQGAGRFTPTDVPSPKLKEFVGKGFNEKQAAIKT
                     VFSDQRVHAVVSEMTHRDMLRENIAASVDHAVSLREQKQLEEYRQATAHLYCHGCGQH
                     CEPAAGGVAVSDILRYLRYHEIYGKRQRAAELYKALPAEARNLAQANLAAAEAACPHG
                     LPIVELIHRADRNMG"
BASE COUNT         1379 a         2056 c         2140 g         1304 t
ORIGIN      
        1 ggccggaccc gacctgctca tgaagctgga gatcgagctc gtcgaagcgg ctcgcggaac
       61 gacgcgggcg atcgacgtga cacgtcaaga cttctgcgcg gaatgccgcg gctcgggcgc
      121 acgcaagggg accgtggcga ccacctgtaa ctattgcgga gggcgcggac agattgtcca
      181 ggctcgaggc ttctttcagg ttgccaccac ctgccccgcg tgcgggggcg aaggtgtgcg
      241 aatcaccgat ccgtgcccta gctgccgagg aggaggacgc gttcccagca cagcgcatct
      301 tcaggtcgac attccgccgg gagttgaaag cgggatgtgg ctgcaactgc gtggtcaggg
      361 agagctgggc gatgtgggcg cgcctcgcgg ccacctccgt atccaggtct tggtcaagaa
      421 gcacccattt tttgagcgac aacgcaatga cctggtatgc caggtgccga tcagttttgc
      481 ccaggcggcc ttaggggcga tggtcgaggt gccaacgctc gatggtcgcg agcatctcga
      541 ggttccccgc ggcagtcaaa gcggcgacgt gctgcgaatc aaggggcgag ggatgcccga
      601 cattggcggc cgagctcgcg gcgacgagct cgtggaaatc gtcgtggaaa ccccgcgcca
      661 tttgacgacg cggcaagaag agctgctacg cgaattcgcc gagatcgagc atcaccaggt
      721 gagtcctcgg cgcaggagtt tcttcgagaa attgagggat tacttcactg aagacgccga
      781 tccggatgat tcgaatgatt cgtaatcgag gtgcagcgtc aggccgacta ttggcggcaa
      841 tccccgagac agtgcaggaa tgagcagcat gaacgaaaaa gcccaaaaac cgaacgatgt
      901 ggacgcctcg ggtgccgagt tcccagaaaa ccgtggccct gatcgagaaa cagggcccgg
      961 gccaaccgag ggccaacctg agaacggtga aacgatgtcg gtttcagaac aaatcgcccg
     1021 gttgaaacac gagcgtgacg agttacgcga tcaggccttg cgtgccaggg ccgaatttgc
     1081 gaactaccag aagcgtgcca aacagcaggc cgaggcagaa cgagcctatg cagtcggctc
     1141 gctggctaag gacctgctcg acccgctcga caacctcgat cgcgccatcg aagcgttgcg
     1201 cgccaagggt gctgaaggca tcacttcggg gcttgacatg gtcctgaagc aacttctcga
     1261 aatcatggcc aagcacggcg tccagccgat atccgcgcac gggcacccgt ttgaccctaa
     1321 catgcacgac gccatcctcc agcagccgtc gctggaacat cccgagggta cagtcgtcgc
     1381 cgagctgagc aagggctaca ccatcgccga ccgcgttctg cggcccagca aggtcgctgt
     1441 ctccgtcaag ccgaccccaa agggcgagta aaagaatgcc tacctacgat tacatctgcg
     1501 actcttgcgg ccacgagttt gaggcgtacg aatcaatcaa ggcagagcca ttgaccgatt
     1561 gcccggagtg caagatcccc cagttgcgcc gcaagatcgg cccgggcgcc gccatcctgt
     1621 tcaagggttc cggcttttat cagaccgatt atcgcagcga gtcgtacaag aaagccgaga
     1681 aagctgaaaa ggcatcgaac gagccggcca aaccagcgtc ggatggggcc agctcaacca
     1741 agagcgagcc ggccaaagcc gcccctgccc cgaccaagag cgagccatga tccggggacg
     1801 ctgcccgatc tgttccaaga gctacgagat cgcgtcggtc gacgccctgc cgtcctttcc
     1861 attctgcacc gaccgctgcc gcatgatcga cctgggccgg tggatcgacg gcaagtatgc
     1921 cattcccggg tcggccaaag accagaagca gcaagaagag ggtgacgaat cctccgcgga
     1981 tgacgtggag tgaaatctcc tcagaatggc acggttatca aaacgccgga agccaatcgt
     2041 ggcaactatc tcggcttact ctcaggcggt tggatgcttg ctttgtcgtc cgtcgtcgcc
     2101 gaccgaataa caccggccaa gaacgcgcaa ccgacgagaa atgacggcaa cccgaccacg
     2161 tactggacgg tggaccagta acgggaccct ggtccttcgt cgtcgaacgc gtaatacaaa
     2221 tcgaaggcgg cgagccaacc gaagtaacat gcgatcgcaa ggacccgcca ggttcggcac
     2281 gtgatgctat cccagtggcg gacggcccag gccgatccga gcacgaaagg caccatctga
     2341 tactgggggt aagccgtgcg atagaagagc acggtcgtaa tggcgactac cacggcggcg
     2401 gcgagcgcgt ccggctgccg aacccacgcc cagcgccatg ctaatagcaa agcagagaac
     2461 aggacgtaag gggccaggta gtcgaggttt gtcatagcac caaacaacag gagcggggaa
     2521 tagcgaccgc ggacgaaata gtagatcgac agcgccgtcg catcccggtt cgcggccagt
     2581 tcgaggggcg acagcgtcga gatcccccag acgagacagc tcagttccat gccgaaggcg
     2641 atcgtactca aggccgaaac gagaaaacgc catcgcacgc ggccgcgatc aacggcaaga
     2701 gctggcagga gcacgatcgg caggtacttg agcaatacgc ctaatgccag gcacgctccg
     2761 gagcggatgt cgtaacctcg cgtccacgct cggaccgctc ccaggcataa gagcccgacc
     2821 aggatgtcga aatgaccccg agcggcgatt tctacccagg ggaatgggtt ccagaacaac
     2881 gcaaaaagca tgatcaactg gatcacggaa ggagcgcgac tggcggtgaa acctttgatc
     2941 tgcgaaatag caaatattac atatgaatat gcacacatca gctttggcgc atacgggttg
     3001 acccaggaca ggcccgtcaa caagttgaag agcggtccgt acgcgttcaa ggggacgtgc
     3061 ccgccggcgc catagacctt gaaccatggg tcatgccctt gggcgatttc ataccacatc
     3121 tggcggtaga agtaatagtc gtgtcgtggc ccggagaggg cagtgacgcc gaggaagacg
     3181 aacgagacga tcaatagcgc gaggctagtg cggttttccg cttggacaac agttgtaggc
     3241 cgcgacgcgg cggctcggag atcatatcgg cgcacgatga aaaagacgct gaagtggagg
     3301 aagatcgcaa gtagcacggc ccccgaatac tcgcgccagc ggccgaggat gtccctggtg
     3361 aggaacaagg cgggcggaag ttcgatgaaa cgcttcaggt aggcgaggat tcccggtgag
     3421 tgctcggcga ttaacagcgg agggacaacc catcgggcga acgccagcca ggcgagcgcg
     3481 aggacggcat agaatatgag cagcgtatgt agctgaggac gatcctgctg agccatcccc
     3541 aaattgtgct gcgtcgtttc gggcctttca actgaccagc tctggctgaa cccagatggg
     3601 gctcaagcgg aagtccgctt cttccgaaag aatgtaccag cgggccgtcg tgcgcgcgct
     3661 gtcattagaa ggctgaaccc cgagatgacg ggtcgccgtc gagcgtttgt ggagcggaca
     3721 aggatggccg tctcaccggc attgcctgaa gtcggtcagg tcgtccgcgt cggcttcgag
     3781 ggaggtccga ctccgcccaa actgagagag gatggggccc gcgcgcggcc gccgcgggcc
     3841 tcgaccatgg cgagactttt cccgcgtgga gagcgaatcg accgacttgt tctggccttc
     3901 gtggcgctcg gtgtggtgct gagagtcggt cactacttga gcaactatcc cctctggggt
     3961 gacgaggcat tcctcgcgat cagctttctt cgtcgcggct acctcgacct actcttgccc
     4021 ctcgaatacg ggcagatctg cccgatcctc ttcctctggg ccgagctaac cgcggtcaag
     4081 ctgttcggat tctcggaaat gtcgttgcgg ctctgtccct tgatatgcgg tgtggggagc
     4141 gttttcctgt tccgccacgt ggccggccgt gtgctgaatg ggcccgcagt tctcatggcg
     4201 gtggccatct tcgccgtgtc gatccatccg atccgccaca gcgccgatgt caagccctac
     4261 gcatccgact tactcgtggc cttaggtctc ttggcgctgg cgatcgagtg gtgtgtgtcg
     4321 ccccagcgga ccggctggct gtggttgctg gtcggtttcg cacccttcgc gctggcggcc
     4381 tcgtatccgg ccgtgttcgt cgcgggggga agcgcgctgg ccctggccgc gtcagtctgg
     4441 aagacgcgac ggcgggggcc gagagttgcg ctcgcggcct acgtccttgt cgtggcgacg
     4501 acgttcgcgg ccctgtttat cgggtatgtg cacggccagc gcggtgcgtc gttgccagcg
     4561 atccagacct actgggccga ttccttcccc ccactcgact cggccatacg gcttgcgagc
     4621 tggctggtca cgacccacgc cggcaatatg tttgcttatc ccgtaggggg tggtcttggg
     4681 gcgagcagcc tgactctgat cctggtgctc atcgccgcga tcgtactctg gaggcgggga
     4741 cacgggacga tcctcgccct ccttgtcatg ccgatgggcg tcgcgctgtc cgcagcggca
     4801 ctcaagctgt atccgtacgg aggcgaggcg cggatcatgc aatacatcgc acccgcggtc
     4861 tgcctgttga ccggcctggc ctctagcacg cttctaggat tgctcccccg gccgactgcc
     4921 cgggccgcgg cgattcgggt tgcggtcatc acgctcgccg tcatggggat tgtcgcgctc
     4981 gtaaacgatt tccgacatcc ttaccggtcc atctacgatc accaggcccg cgagttcgcc
     5041 cgccgattct ggccagagca ggcccgaggt gccgaactcg cgtgccttca atgggactta
     5101 gggatttgtc agcggggtgc cgccgttgcc cgcacggcca tctacttgtg taatcagcat
     5161 atttattcgc cgaagaggag acgaggcctg ggaccgcgct gggcactcgt gacgcttgac
     5221 cggccgctcc gttgcgtgtc tttcgacgcc gttcatctca aaggtccgaa ggcgactgcc
     5281 tggctcgaat cgatgagagc aaattacaat ttccgagggc agctcgatct ggtcgtccca
     5341 atcacgcgcc ttgatatgaa gccggggaat gaccacgtct ttgtctttga gttccagccc
     5401 aagtctgacc ggccgatcga acgcgtcgtc accggagtcg cgacagaccg gactgtgagg
     5461 tgatgctcgt tttctcgtcc aacagaaaag gacgacctcg catcatgcgg gaatgatgca
     5521 acgtcgtcct tcattttcct gaccaatcgg ggatctgttc cggattaaac aggtccttag
     5581 cccatgttgc gatcggcccg gtggatgagc tcgacgatcg gcaaaccatg cgggcaggcg
     5641 gcttcggcgg cggcgaggtt ggcctgggca aggttgcggg cttcggcggg caaggccttg
     5701 tacagctcgg cggcgcgctg acgcttgccg tagatctcgt ggtaccgcag gtagcggaga
     5761 atatccgaca cggccacgcc gccggcggcc ggctcgcagt gttgaccgca gccgtgacag
     5821 tagaggtgcg cggtcgcctg gcgatattct tcgagctgct tttgttcacg caaactgacc
     5881 gcgtggtcaa cgctggcggc gatattttcc ctgagcatat cgcggtgggt catctcgctg
     5941 acgacggcat gaacccgctg gtcagaaaag accgtcttga tcgcggcttg cttctcgttg
     6001 aaacccttgc cgacgaattc cttcaatttc ggtgatggga catcggtcgg cgtgaaacgt
     6061 ccggcgcctt gcgtcttcat ggcgacgatg gccaagtttg ccttcgatgc cgcatcgagc
     6121 gccttcttga cggcgtcggt ggtcatggtc cggtagttgt actggatcat gatctggtcc
     6181 atccagccgg ccgcggcggc ggccgtgacg atctcgggca gccgaccatc atggcaggac
     6241 agaccacaga acttgatctt gccagccttt ttcagtttct caaacgccgc cttgacgtct
     6301 gggtctttga gcaacgggat ctcgcgacct gtgacaccgt ggaggtagta gcaatcgatg
     6361 tagtccgtcc gcagccgctc catgctcgcg gtcaaatgtt tctcgaacgt gggaaacgct
     6421 ctggaaccac ccaccttgcc gccgctgttc ttcgtgacca ggtaaacttc tttacgcatt
     6481 ttggtgcgtt cgagcacctc gcccagtgtc ttttccacgt tgcccgattc ataggcctcg
     6541 gatgtatcga tgtagcgcac gcccgcgtgg agcgcggcct gaacgaagct gggcgcgact
     6601 tcccaactcg ttcccatgcc caggacgggt attttttcac cggttttgcc caacgtcacc
     6661 ttggggatcg tcgaggccgc gggctgtgcc caggccgaat cggctgagcc aattgcggcg
     6721 gtcccgccga cggcggcggc cgtcttgaga aaatttcgcc gactgccttc attcccatga
     6781 tcgttctgga aactgtccat cttgatcgcc ctttttcaag gagttcagcc gatccccttc
     6841 acacagtccc cggcccttgg gtcgacaatg aattcagtc
//
LOCUS       DATAQL010000349         6047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4614297, whole genome shotgun sequence.
ACCESSION   DATAQL010000349 DATAQL010000000
VERSION     DATAQL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4614297"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..406)
                     /locus_tag="VHS97_06005"
     CDS             complement(143..406)
                     /locus_tag="VHS97_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447786.1"
                     /translation="MTGNPVGRVALDPSATIGSAFNVEGIPTLVILDAKGIVQSVRVG
                     YDPDAPEELSKTLAKEIDSLLAGKSLVGPKDGAKQASKKDVEK"
     gene            complement(473..1603)
                     /locus_tag="VHS97_06010"
     CDS             complement(473..1603)
                     /locus_tag="VHS97_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3447787.1"
                     /translation="MLLSRQTWPRWIRVSRVAIAGASVLGLGLATLARAQAPETKTLE
                     LRTSKPETKRDDPKARALFDEVTKAYKGLTSYSDQGQFIIAMSMGGKPQKQVLPLKLT
                     LVRPNKLEMDAGQVKINSDGKTMTTTVIPLKRYSTTAAPEKIGIETFREGPLGSVLFG
                     GPSGPPMFLLLNLLTAADPAAAVAQIGGSMQAAPAAAPKADGAAKAESSAVLIDLAEG
                     QPDILLTIDPATKLLSGIDMKIDPKLVAQAAKGGQELAIDQFGWSAGAVSTEVPKDRS
                     FAYEAPKGFAKVDSILEPPAEKPSSALLGKPAPDFTFTLLDGPGKTKTVTKAELAGKV
                     VVVDFWATWCPPCMKELPEIQKLIDSFANTDKNVLVVAVSQD"
     gene            complement(1802..3046)
                     /gene="ribB"
                     /locus_tag="VHS97_06015"
     CDS             complement(1802..3046)
                     /gene="ribB"
                     /locus_tag="VHS97_06015"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX3447788.1"
                     /translation="MNNEFSQIDDALQALREGRIIIVVDDEDRENEGDFVAAADKVTP
                     EVVEFMITHGRGLVCMPILPEVAARLHLAPMVDRNTAPHQTPYTVPVDHRSCHTGISA
                     EERARTVRAIVDPTTVPEDLARPGHLFPLVAKEGGVLRRAGHTEATVDLVRMAGLTPA
                     GILCEILDGTGVASRDRLHAIGREHGLPIVSIEALIKYRRLREKLVSRVAEADLPTRY
                     GPSRIIGYRVQHEPGNEPVAIVMGDLRKVDAPLVRLHSSCFTGDLLDSLRCDCGDQLH
                     MALEMIGAQGAGALIYLPQEGRGIGLIEKIRAYSLQDEGLDTVQANTALGYRADPRDY
                     GIGLQILKDLGLSTVRLLTNNPKKTDAFVYYGYDLKVVDQVPIVAPPVAERRRYMEAK
                     RDKMGHVLPSHGGRENGAASNH"
     gene            3270..4109
                     /gene="proC"
                     /locus_tag="VHS97_06020"
     CDS             3270..4109
                     /gene="proC"
                     /locus_tag="VHS97_06020"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX3447789.1"
                     /translation="MSRWGFVGSGKMATALVKGMLRANVASIEEIRASDPLESARHQF
                     QAETGVAVFDSNVPVVQLSDVVVLAVKPQSMRLVLENVRPFITHDHLIVSIAAGITIA
                     SIVQGLGSQARVVRVMPNTPALIGEGASAYALGPGVSADDERVVKAFLDSVGRTVGVA
                     ELLLDAVTGLSGSGPAFVYVMIEALADGGVRVGLPRDVATLLATQTVLGAAKMVRDTG
                     QHPGWLKDQVASPGGTTIAGLHALEQAGLRGALMNAVEAATRRSTELAALAGPPTDDR
                     TGR"
     gene            complement(4124..4726)
                     /locus_tag="VHS97_06025"
     CDS             complement(4124..4726)
                     /locus_tag="VHS97_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3447790.1"
                     /translation="MSTATFVRSAVNPGVISSAEPMETDEAFYETVHGQRVEMPPMSV
                     RASIVASRISIALGAFARPNRLGEVFTELLIHVPLPEDASRDRRPDVAFFAYPRSPST
                     STQLPNVNAWDVIPDLAIEVTSPSDRAEAQREKVLDYFRAGVRLVWVVYPDLRLIDVY
                     ETPTTIRVFGPDGTLAGDPVLPGFELSFADLFAPFAPPSA"
     gene            complement(4799..>6047)
                     /locus_tag="VHS97_06030"
     CDS             complement(4799..>6047)
                     /locus_tag="VHS97_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447791.1"
                     /translation="TIAWRINRPGHVRDVEIELGRQLGLQVTLDAVRYPKPGEVVYQG
                     ITLRQAEPRSKGLTEIARASQARLQRADRELILYLENPRLSADSPKLGLGQVAAMLQR
                     SGQIPFERVHVTAGSCQLDFGHESLRLALRDVAGEFVVDPLTPSVKLTYRVPGVGAGT
                     RCELTVTRDRRTEQIETSVALTTVEGLPLPARLLNVFFDADDWLGSSAKFEGALRLHQ
                     AGSREWEADFTGELLDVDLAKLVGRRFPRHRLTGRARVAIKEAKWGQRPTQGWGWLEA
                     KGELLAGHGSIGVDLIEALAREMKFRRSPRLANLDARKTDVDFRALGLSFAMESNGEI
                     QIAGALGAEFSPDAVIAGATTSLLSAPQGTASVHGLIKTLFPVSQASSGVLVPLTAES
                     QLLLSLPVSPGSVTTTRQTVDGN"
BASE COUNT         1139 a         1871 c         1866 g         1171 t
ORIGIN      
        1 ctcgacccca gccacccggc tcttgtcacc tccgaagttg ctaaagttga ataatgcccg
       61 aggccagatc gtcgaacaaa aaaggccggc ctcccgtagc tccggagacc ggcctttctg
      121 tttatgtcca cagtaatcac ggctattttt cgacatcctt ctttgaggcc tgcttggccc
      181 catctttggg accgaccagc gacttgcccg cgagcaagct gtcgatttcc ttggcaagcg
      241 tcttgctcaa ttcttcgggc gcgtcaggat cgtagccaac gcggaccgac tgaacgatcc
      301 ctttggcgtc cagaatgacg agcgtcggaa ttccctcgac attgaaggcc gagccgatgg
      361 tggcgctggg gtcgagcgcg acgcgtccca ccgggttgcc ggtcaagttt atcttctttt
      421 ccgtgagggt cttttcgacc agcttgcgga cttccgagat ctcggcgggc cgttaatcct
      481 ggctcacagc gacgacgaga acattcttgt cggtgttggc aaacgaatcg atgagcttct
      541 gaatctcggg cagttccttc atgcatggcg ggcaccatgt tgcccaaaag tcgaccacga
      601 cgaccttgcc ggcgagctcg gccttggtca ccgttttggt ctttccgggt ccgtcgagca
      661 gggtgaacgt aaagtcgggg gcgggtttac ccaggagcgc gctacttggt ttctcggccg
      721 gcggctcgag gatcgagtcg accttggcga agcccttggg tgcctcgtag gcgaacgatc
      781 ggtctttggg gacttccgtc gaaaccgcgc ccgcagacca gccgaactga tcgatcgcca
      841 gctcttgccc acccttggcg gcttgagcta caagcttcgg gtcgatcttc atgtcgattc
      901 cggaaagcag cttcgtggcc ggatcgatgg tcagaaggat atccggctgc ccctcggcga
      961 ggtcgatgag aaccgccgag ctctctgcct tggcggcacc atctgccttg ggcgcggcgg
     1021 ccggagccgc ttgcatcgag ccgccgatct gagcaaccgc ggcggccggg tcggcggccg
     1081 tcaggaggtt caacagcaag aacatgggcg gtccactggg accaccaaag agcaccgagc
     1141 cgagcggtcc ttcgcgaaat gtctcgatac cgatcttctc gggagcggcc gtcgtcgaat
     1201 agcgcttgag cggaatgacg gtggtcgtca tcgtcttgcc gtcgctgttg atctttacct
     1261 gaccggcgtc catttccagc ttgttggggc gaaccagggt caacttcagc ggcagaacct
     1321 gcttctgagg cttgcctccc atcgacatcg caatgatgaa ctgcccttgg tcggaatacg
     1381 aagtcaatcc tttgtaggct ttggtgacct cgtcaaagag tgcgcgagct ttgggatcgt
     1441 cgcgctttgt ctccggcttt gaggtcctga gttcaagggt cttggtttct ggcgcctggg
     1501 cccgcgccag cgtggccaga ccgagcccca acacggaggc tccggcgatc gccactctgc
     1561 tcacgcgaat ccagcgcggc catgtctgtc gactcaacaa catgaggaat cctcggatgg
     1621 ggaaggtaga aaagccagac cggcgaccgc gcccgcggcc cgctcgccca tccgactggg
     1681 gagaattact tgagtctatc catttgcgta gccttacgtc gagccgacta gaccagccgg
     1741 caattacccc aaatttgacc aatttaccgg caaatcctgg gccggcggaa ataaaacaag
     1801 ctcagtggtt ggatgcggcg ccattctccc ggccgccatg agacggcaac acatgtccca
     1861 tcttgtcgcg tttggcttcc atgtaccgcc tgcgttcggc gaccggaggt gccacaatcg
     1921 gaacttgatc aaccaccttg aggtcatatc cgtaatagac gaacgcgtct gtcttcttgg
     1981 ggttgttggt caggagacgt accgtcgaaa gcccaagatc cttgagaatc tgcaagccga
     2041 tgccgtaatc gcgcgggtcg gctcggtaac caagcgcggt gttggcctga acggtatcga
     2101 gcccctcatc ctgcagacta taggcgcgaa tcttttcgat caggccgatt ccccggcctt
     2161 cctgaggcaa gtagatcaag gcccctgccc cttgggcgcc gatcatttcc aaggccatgt
     2221 gaagctggtc gccgcaatcg caccgcaacg aatcgagtag atccccggtg aagcacgatg
     2281 agtgcaatcg caccaggggt gcgtcgactt tgcgaaggtc tcccatgacg atcgcgaccg
     2341 gctcgttacc cggctcgtgt tgaactctgt agccgatgat ccggcttggc ccgtagcgtg
     2401 tcggcagatc ggcctcggcg acccggctca ccagcttctc gcgcaggcgg cggtatttta
     2461 tcaaggcctc gatcgagacg atcggcagcc cgtgctcgcg gccgatcgca tgcagccgat
     2521 cacggctggc gacgccggtg ccatcgagga tctcacagag gattcccgca ggcgtcaatc
     2581 cagccatcct taccaggtcg acggtcgctt cggtatggcc agcccttctg agcacgcccc
     2641 cctccttggc gaccaaggga aagagatggc ccggccgggc cagatcctca ggcacggttg
     2701 tgggatcaac gatggctcga accgtccgcg cccgctcttc ggcactgatg ccggtgtgac
     2761 acgatcgatg gtccacggga accgtgtacg gggtctggtg cggggccgta ttccgatcca
     2821 ccatcggggc aagatgaagc cgggccgcga cctcaggcag gatcggcatg catacaagcc
     2881 cgcgtccgtg cgtgatcata aactcgacca cttcgggcgt aaccttgtcg gcggccgcga
     2941 caaaatcccc ctcgttttcg cggtcttcgt cgtcaacgac gatgatgatc cgcccttccc
     3001 tcagtgcctg gagggcgtcg tcaatctgcg agaattcatt gttcatggcc agttcctgac
     3061 ctcgaagagg cgatattgca tcgaacttca ttctacccag tcgggcaact cgcggcgcgg
     3121 tccgccataa ggcgcgacag ctctgggctt cttgtcgatc actcccgcgg gggctattct
     3181 gcaccaagcc gagaggttcg agttcgcgaa cgatgattgg cctttgggcc ctaaacagcg
     3241 cggccagcga ttcgccggag acaaataaaa tgagccggtg gggctttgtg ggttcaggga
     3301 agatggcgac ggcgctggtc aaaggtatgc tccgtgccaa cgtcgcatcg atcgaggaaa
     3361 tccgggccag cgaccccctt gagagtgcgc gccaccaatt ccaggcggag accggagttg
     3421 cggtctttga ctcgaatgtg ccggttgtgc agctgagcga tgtcgtggtg ctggccgtca
     3481 agccgcagag catgcgcctg gtgctagaaa acgtgcgacc gttcatcacg cacgatcacc
     3541 tgatcgtctc gattgcggcc gggatcacca tcgcgtcgat cgtccagggt ctgggaagcc
     3601 aagcgagggt ggtccgcgtg atgccaaata caccggccct gattggtgag ggtgcctcgg
     3661 catatgcgct gggccctggc gttagcgcgg acgacgagcg ggtggtgaag gcgtttctcg
     3721 actcggtcgg ccggacggtc ggggtggccg aattgttgct cgatgccgta actggactct
     3781 cagggagcgg acccgccttt gtttatgtca tgatcgaggc gctcgcggac gggggtgtca
     3841 gggtcggttt accgcgcgac gtcgccacac tcttggcgac ccagaccgtg ctgggggcag
     3901 caaaaatggt gcgtgacacg ggtcagcacc ccggctggct caaggaccag gtggcgtcgc
     3961 ccggcggcac cacgatcgcg gggctgcatg ccctcgagca ggccggcctg cgtggggcgt
     4021 tgatgaacgc ggtcgaggct gccacacgcc gctcgactga gctagcggcg ttggctgggc
     4081 cgccgactga cgatcgcaca ggccgctagg cgagcggaat ctttcacgca ctcggtggcg
     4141 cgaacggtgc gaacaggtcc gcgaacgaca gttcaaatcc aggaagtacg ggatcgcccg
     4201 ccagcgttcc atccggaccg aagacgcgaa tcgttgttgg cgtttcgtag acgtcgatca
     4261 accgaagatc gggatacaca acccaaacga gccgcacgcc agcccggaaa tagtcgagta
     4321 ctttttctcg ttgagcctcg gccctatcgg acgggctcgt gacttctatt gccagatcgg
     4381 ggatgacatc ccacgcgttg acgttcggaa gctgggtact ggtcgacggc gaccgaggat
     4441 aagcgaagaa cgcgacatcg ggccgtcggt cccgactcgc atcctcgggc aaaggcacgt
     4501 ggatgagcaa ttccgtgaat acctccccca gtcgattcgg cctcgcgaac gcgcctagcg
     4561 cgatgctgat tcgggaagcg actatcgagg cgcgaacact catcggcggc atctccacgc
     4621 gttgcccgtg cacggtttcg tagaaagcct catccgtttc catcggctcg gccgaggaaa
     4681 tgacgccggg attcacggcc gatcgaacga aggtcgcggt gctcatcgga tgcctcccaa
     4741 gggggtcgat tcgctccaat tctagcagag ggccacgctc cggagtacga atctttgctc
     4801 agttgccgtc gacggtctgc cgagtcgttg tgacggaccc cggagagacg gggagcgaca
     4861 gcaacagttg cgactcagcg gtaaggggga ccaacactcc ggaactggcc tgagaaacgg
     4921 ggaagagcgt cttgatcagg ccgtgcacgc tggccgtccc ctggggggca gacagcagcg
     4981 atgtggttgc accggcaatc accgcatcgg gggaaaattc cgcgcccaag gcgccggcga
     5041 tctggatctc gccgttggat tccatcgcaa acgaaagtcc gagcgcccga aagtcgacat
     5101 cggtcttgcg agcgtccaga ttcgcaaggc gcggcgagcg gcgaaatttc atctctcggg
     5161 ccaacgcctc gatcagatcg actccgatcg agccgtgtcc ggccagcagc tcccccttgg
     5221 cctcgagcca gccccaccct tgcgtcggcc gctggcccca cttggcttcc ttgatcgcca
     5281 cccgggctct ccctgtcaat cgatgacgcg gaaagcgccg gccgaccagc ttcgcaaggt
     5341 cgacgtcgag caactccccc gtaaagtcgg cctcccactc tcgtgatcca gcctggtgca
     5401 agcggagcgc accctcgaac ttcgcgcttg atcccagcca gtcatccgcg tcgaaaaaca
     5461 cgttgagcaa gcgggccggt aacggcaagc cctctaccgt cgtgagcgcg acggaagtct
     5521 caatctgctc ggttctccgg tcgcgtgtca ccgtcagctc gcaacgggtg ccagcaccca
     5581 cgccgggcac gcggtacgtc agtttgaccg acggtgtcag agggtcgact acaaactcgc
     5641 cggcgacatc gcgcagagcc agccggaggc tctcgtgacc aaagtcaagc tgacatgagc
     5701 cagccgtgac gtggacccgc tcgaaaggga tctgtccgga tcgctgaagc atggccgcga
     5761 cctggcccag cccgagcttg gggctatcgg cggacaaccg cgggttctcg aggtaaagaa
     5821 tgagttcccg gtccgcgcgt tgcaagcggg cctgactggc ccgggcaatc tcagtcaggc
     5881 ccttgctgcg gggttctgcc tgccgcagag tgattccttg ataaaccact tcgcccggct
     5941 tgggataacg aaccgcatcc agcgtcactt ggagcccgag ctgacgcccc aattcgatct
     6001 cgacgtcgcg gacatgaccc ggccgattga tccgccaggc gatcgtg
//
LOCUS       DATAQL010000350         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1103022, whole genome shotgun sequence.
ACCESSION   DATAQL010000350 DATAQL010000000
VERSION     DATAQL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103022"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1244..1519
                     /locus_tag="VHS97_06035"
     CDS             1244..1519
                     /locus_tag="VHS97_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447792.1"
                     /translation="MPNEAERRYGVGSEEANDAFERGGPACHAAFDAAYSLASTPPTT
                     LVGVLAVLRFVSEIEDQGQWREDTGLERKLLATTAQATQTIIRQGVV"
     gene            1704..2015
                     /locus_tag="VHS97_06040"
     CDS             1704..2015
                     /locus_tag="VHS97_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447793.1"
                     /translation="MLRGSPLRDCIGTISFGATVVGKMHTAGLKGEPLATIQTKTRKE
                     WLETLDRAFEAVATLSVGLCQAMNTLKALQSETSDLPVNLPVPDLGQSVLNLATEPDP
                     A"
BASE COUNT          519 a          805 c          759 g          511 t
ORIGIN      
        1 ctgccatggg tctgccaact gggcagccgc aaattgccgg cattcagaac tcaatgtctc
       61 agaacagtgc ccagcgcgcc ggcagcgctg acgacgtgaa gatctttaaa agaatccagg
      121 gagtgaggag gaagacgagc attacgctac ctcgctggta ttggccgccc cattcggagg
      181 gctgctcgat accgtgtacc tgattcgcag tgcggatgac ctacgttacc gcgagatttc
      241 tgcgggcgag cgctagcgga tttcccaagc gtcacagatt aatccatgcg tgtatcttgg
      301 tgcggcgctg cgacggctct gtgcggtcga aaaacgcctc tgccagttct ctcctctttt
      361 gagccttatc gtttccaact tggacaggta agggtcgacg atgctgtcga catgaggcct
      421 catgagcgga tggtctgaat tcagaaggtc gacgagttca ccgaggcatt cttccgaggc
      481 atccacttct tcgcctgcag gccgacctcg atcctcgaga gtttgctcca gatcatttcc
      541 ctccctgcct cgacgtcggc gcacgagccc gacggttccc atcaagaccc agactacgcc
      601 gccagcgatc agcagatacg ccgagatctc ggcgtcgctc atttcttcgc cctcagcccg
      661 atttcgacca gccggcggat ggcttcagag cggcccgttc cgtttgcttc ggcccacgcg
      721 ttcgcctcgg caatgaggtc gggcggcatt cggatcgcaa ccatggggtc acgaccgcca
      781 gaaggtggcc ttcctcgtgc tctctttttt ggtttgaccg ttattggctt tttcataaat
      841 aaggtattac cataaatgcg agccgagggg aaagttccag cttccgcacc ggctctaacc
      901 cgagccacca aggagcacaa tccaaatgac ccaggctgac agcgtgcata gcacgccgcc
      961 cacgaactcg tcctcaattc aggcaatcaa tccccggcac aagcccgtgc ggagtcgaag
     1021 gattcgataa tccgtttatg cccaccggtg accagccgga cgaaaggtct gccagtgact
     1081 cccgcaagcc cgctgagggc ctatcccgcc ggctcacgct ggcagggatc gccgtggtgg
     1141 ggatcccacc acggctctgg ctgccgccgc ggagcctgac ccggtcttcg cgctgatcgc
     1201 ggccaagcgg gcggccgacg tcgtccatct cgattcccgt cacgtgccga acgaggccga
     1261 gcgccggtat ggcgtcggtt cagaggaagc caacgacgcg tttgaacgcg gcggtccagc
     1321 gtgtcacgct gcctttgacg cggcctattc gctggcatcg acgccgccga caacgcttgt
     1381 cggcgtcctc gcggtgctca gattcgtcag tgagattgag gatcaggggc aatggcgcga
     1441 ggacacgggc ctggaacgca agttgctggc gaccacagcc caagcgaccc agaccatcat
     1501 ccgccaggga gttgtgtgat ggccgagatt ttcaaatttc cctacgacgc ctgtcgtcgt
     1561 gtccattccc gcaagcctcg ccgatcgaag aatggcacgc ccgaggagcg ggcggcaaag
     1621 gcagcggcgt tggaaacaac gccggccgat gttgtcgcca tgtcggacgc tcgaacgcaa
     1681 gcccgttcgg aagtcgatgg cggatgcttc gcggcagtcc gttgcgtgac tgcatcggca
     1741 cgatctcgtt tggtgcaaca gtggtcggga agatgcacac tgcggggctg aagggtgaac
     1801 cgctcgcgac gatccaaacc aagaccagaa aagagtggct ggaaactttg gatcgcgctt
     1861 ttgaagccgt ggcgacgctc agcgtcgggc tttgccaggc gatgaacacc ttgaaggcgt
     1921 tgcagtcgga aacttccgat ttaccggtaa atttaccggt ccctgactta ggacagtctg
     1981 tcctaaattt agcaacggag cccgacccag catgaagcgc cgctgtcgat cgcataggaa
     2041 gcgcacccgg gcgcggccgg cgggccaccg ccgtcgactg tcccgctgac aatcccaaaa
     2101 agatacgccg ccgggctcga aatacaccgt cccggcggcg ccctgtttca aactgggccc
     2161 tgcaatcccc agcatcactt ctgtagcaat cgacatgcca tcggctggct acacatgcca
     2221 ggaggctgag gcggtagctg gattgcaacc gatccgcaat aatctgtgac tcgcctaccg
     2281 aacgaaggcc tcatacctat tgccccgctg acgcgagcgt gccggcgggt tttctttgag
     2341 tgtggggcat gtcttggtcg ccaaaatcga cgtacatcgc ggaaagccga atcgcgttaa
     2401 ttgggctgcg cttgtggcgt tcatggcgct tatcgtgccg gcgctctgtg cttgttctgg
     2461 tctgtcccag cctgctccat catcgtcgcg ctacctcgac ctaaacgatc cgccgccacg
     2521 ccgtgacctc atggccgatc aagaaacagc acgggcaaaa gccgagctga ccgacaggcg
     2581 tgacaacgca gagc
//
LOCUS       DATAQL010000351         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4766728, whole genome shotgun sequence.
ACCESSION   DATAQL010000351 DATAQL010000000
VERSION     DATAQL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4766728"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VHS97_06045"
     CDS             <1..441
                     /locus_tag="VHS97_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447794.1"
                     /translation="LVALTASRADADDVLQETFVRLARMRKSLAAVDNLVAYVFATAR
                     NESIRLAQRQVREDQVRVGAELVARSCEVPVEDPEALETAEWVAAALARLSDELREIV
                     ELKIYAELTFREISAVTGLPQGTVATRYRSALEKMRNQMVKEQQ"
     gene            438..974
                     /locus_tag="VHS97_06050"
     CDS             438..974
                     /locus_tag="VHS97_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447795.1"
                     /translation="MNDDIERMLRQVRPRAASAELRHRVLAAVAIELGADAPLPSRLL
                     RRPGLVVAAALIMSLALNYWVNASLDHRLAVVLGPSPVDRQTAEIAADIASITDTYTA
                     EWAYRRLAAARPARDEARQYSVRLQQMVRQVIVYLKESADESIQKKPQMDRDRDRSDN
                     RPPAGAQRLLRLEHRNSA"
     gene            862..2133
                     /locus_tag="VHS97_06055"
     CDS             862..2133
                     /locus_tag="VHS97_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447796.1"
                     /translation="MNRFKRNLKWIAIATGATIALLLVLNAYFVWNTGTRLERRLAEL
                     RQAGDPVQIADFARAPVPPEKNADVYLRRATSDLDAIQKELMVWYPKTIYPTGTLSAP
                     EKERLEKLFAAYSQLMPLLEQAADSPDYDPQLDYTLSPTRFLEPCMKHSSEHRVLYRV
                     LRARCALLLAQGHNDEALATQLLMLRLTRHWRREPFLLNYLVTAVCEHLAMGGLNQVL
                     QTGPVSSSARQALDAELALHDTIEGYRWALRNERAYSLSSLREMPGDRFWLTRGFAND
                     LMLRLIHLFDRYLEKASRLYADAIADKSHVSMPGGVPNVYGALVTLLEPALVSAREPS
                     ERVRALSRSLRVLNALQARAPSDDNSVPNIGDLALPTEATIDPFNGKSLIVKKAPEGW
                     TVYSVGSDLVDNGGKLDGRTDVGAGPNSAAN"
     gene            2306..>3192
                     /locus_tag="VHS97_06060"
     CDS             2306..>3192
                     /locus_tag="VHS97_06060"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX3447797.1"
                     /translation="MARRVVITGLGTVNPLAKDVRRYWDGLCNGRSGIGPIELFDASA
                     FKVRFGGEVKGFDPENVLDPKMARRMDRFSQFAVTAALEALEDSGVDMEKEDPYRWGV
                     TLGCGIGGLATFEEGHGRLLEAGPGRLSPFVIPRMMPNAGPANIAIHLGMRGPSWAVA
                     SACSSSGDAIGAACRAIRCGDADAMLAGGAEAALTPIGLGGFVAARSLSLRNHDPRAA
                     SRPFDRDRDGFVLSEGAGVVVLESLEHARARRATIHAELLGYGSTNDAYSITAPHPDG
                     DGAARAIELALADARMDRS"
BASE COUNT          609 a          955 c          983 g          645 t
ORIGIN      
        1 ctggtggccc ttacagcgtc gcgagctgat gccgacgacg tgttgcaaga gaccttcgtc
       61 cgcctggccc gcatgagaaa gagcctggca gcggttgata acctggttgc ttatgttttt
      121 gccactgccc gaaacgagtc aatccgactg gcccagcggc aagtccggga ggaccaggtg
      181 cgggtaggcg cggaattggt ggcacgctct tgcgaggtgc ctgtcgagga ccccgaagcc
      241 ctcgagacag cggaatgggt cgccgccgct ctcgcgcggc tgagcgacga acttcgcgag
      301 atcgtggagt tgaagattta tgcggaactg actttccgcg agatcagcgc ggttaccggg
      361 cttcctcagg gtacggtcgc cacgcgctat cgatcggccc tcgagaagat gcgcaatcag
      421 atggtgaagg agcaacaatg aacgacgaca tcgagcgcat gcttcggcaa gtcaggcccc
      481 gggccgcatc agcggagctc cgtcatcgag tgctggccgc cgtggctatc gagttgggcg
      541 ctgacgctcc cctaccctcc cgcctgctcc gccgtcctgg actcgttgta gcggctgctc
      601 tgattatgag cctggcgctg aactattggg tgaatgccag cctcgatcat cggctggcgg
      661 tcgtgctcgg tccgtcgcca gtcgacagac aaaccgcgga gatcgccgca gacatcgctt
      721 ccatcaccga cacctatacg gccgaatggg cgtaccggcg actcgcggca gcgcgacccg
      781 cccgtgacga ggcccgccag tattcggtcc ggctccaaca gatggtcaga caagtcatag
      841 tttatctcaa ggagagcgcc gatgaatcga ttcaaaagaa acctcaaatg gatcgcgatc
      901 gcgacaggag cgacaatcgc cctcctgctg gtgctcaacg cctacttcgt ctggaacacc
      961 ggaactcggc ttgagcgccg actcgcggag cttcgccagg ctggcgatcc ggtgcagatt
     1021 gccgacttcg cccgcgcgcc ggttcctccc gagaagaacg ccgacgtgta tctccgccgc
     1081 gcgaccagcg acctcgacgc tattcagaaa gagctcatgg tttggtatcc caagacgatt
     1141 tatcccacgg ggacattgtc ggcgccggaa aaggaaaggc ttgaaaaact ttttgcggcg
     1201 tattcccagt tgatgccgct cttggagcag gccgcggatt cacccgatta tgatccgcag
     1261 ctcgattaca ccctgagtcc gacccgcttt ctggagccgt gcatgaagca ttcgtcggag
     1321 caccgtgtgc tttatcgcgt tttgcgggca cgctgtgcct tgctgctggc gcaaggccac
     1381 aacgacgaag cgctggctac ccagcttttg atgttgcggc tgacgcgtca ttggcgccgc
     1441 gagccgtttc tcctcaacta tttagtaacc gcagtttgcg aacacttggc catgggggga
     1501 ctgaaccagg tgttgcaaac cggcccggtc tcttcatccg cgcgccaggc gcttgacgcc
     1561 gaactcgccc ttcacgacac gatcgaaggc tacagatggg cactccggaa cgaacgagca
     1621 tactcgctgt cgtcgcttcg agagatgcct ggcgaccgct tttggctgac gcgcggtttc
     1681 gccaacgacc tgatgttgcg gctaattcat ctcttcgatc gctaccttga gaaggcctcg
     1741 cggctttacg ccgatgcaat tgccgacaag agccacgtct ccatgcccgg cggcgtaccg
     1801 aatgtgtatg gagcccttgt cactctcctt gagccagcct tggtctcggc ccgcgaacca
     1861 agtgagcgcg tacgcgcctt gtctcgttcc cttcgcgtgc tcaatgctct tcaagcgcgt
     1921 gctcccagcg atgacaatag tgttcccaac atcggcgacc tcgcactgcc gaccgaagcg
     1981 accatcgatc cgtttaacgg caaatcattg attgtgaaaa aggcgccgga aggctggacg
     2041 gtctattcgg tcggcagcga tctggttgac aacggcggca agctcgacgg aagaaccgat
     2101 gtcggcgccg ggccaaacag cgccgcaaat tagttgggtc gaatcagcga tcggcccttc
     2161 cttgacaagg tccacacacg gatgcgcttc ctatgggtcc gacttgaatt taaatgacgg
     2221 tcgtcataca atcgtgccga tactcggtaa cgcgtaaata gactctatta ccgaggatga
     2281 ttgccggcgg gaaacgaggt tgccgatggc tcggcgtgtt gtgatcaccg gcttgggcac
     2341 ggtcaatccg cttgccaagg atgttcgccg ttattgggat ggactctgca acggccggag
     2401 tggaatcggc ccgatcgagc tattcgacgc gtcggcgttc aaggtgcgat tcggcggcga
     2461 ggtaaaagga ttcgatcccg agaatgtcct tgatccgaag atggcccgca ggatggatcg
     2521 gttttcgcaa tttgcggtca cggcagctct cgaggcacta gaagacagcg gcgttgacat
     2581 ggagaaagag gatccgtatc ggtggggtgt cactctggga tgcggaattg gcgggttggc
     2641 tacatttgag gaagggcatg gccgccttct ggaagccggt cccgggcggt tgagtccatt
     2701 cgtcatccct cggatgatgc ccaatgccgg tcccgccaat atcgcgatcc atctggggat
     2761 gcgtggaccc agttgggcag tagcctcggc gtgctcttct tcgggcgacg cgattggcgc
     2821 ggcctgtcgc gcaattcgtt gtggggatgc cgacgccatg ctcgcgggag gtgctgaggc
     2881 cgcgctcacg ccgatcggtc tgggcggctt tgtcgccgcg cggtcgctct ccttgcgcaa
     2941 ccacgacccc cgggcagcca gccggccttt cgaccgcgac cgagacggtt ttgttctctc
     3001 cgaaggggct ggggtggttg tgctggaatc gctcgaacat gcgcgagccc gcagggcgac
     3061 cattcatgcc gaactcctcg gttacggcag taccaacgat gcttacagca tcacggcgcc
     3121 gcatcccgac ggggacggag cagcaagggc catcgagctt gccctcgcgg atgcccgaat
     3181 ggatcgaagc ga
//
LOCUS       DATAQL010000352        10107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1408732, whole genome shotgun sequence.
ACCESSION   DATAQL010000352 DATAQL010000000
VERSION     DATAQL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408732"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHS97_06065"
     CDS             <1..223
                     /locus_tag="VHS97_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447798.1"
                     /translation="ALLDHPVGRLPLLSHCVHYDYTKLRQRKSAALMTQADGPSLHLS
                     HWYFDGHIDVAHRRRISPNRKLARMVGCI"
     gene            483..1691
                     /locus_tag="VHS97_06070"
     CDS             483..1691
                     /locus_tag="VHS97_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013562972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX3447799.1"
                     /translation="MESSVCIVGGCGHIGLPLGMALAEVGVPVTLFDIDVARVRLVAA
                     GQMPFLECGAERILPHLLSTDRLKVTTSPEVIRQHDIVVVTIGTPVDEFMDPNVRSFD
                     RAIDHVLDHMSDGQLLILRSTVFPGVTTRLDRRAAERGLRIDIAHCPERIAQGYALEE
                     LTKLPQIVGGVTAVASQRSVALFSLLGTKVIKLPPIEAELTKLFSNAYRYISFAISNQ
                     FYAIAENLGADFKRVRQAMIADYPRMAGFPAAGLAGGPCLLKDTMQLAAFNHNAFILG
                     QAAMMVNEGLPSILVEAVKAKYSMTGSTAAILGMAFKGNSDDPRDSLAYKLRKVLTLE
                     CRRVLCSDPYIDDPSFVSVETALKEADFVFLGACHDEYRNLVIEKPVIDVFGFLLQNV
                     KTALEKRSAA"
     gene            1764..2786
                     /locus_tag="VHS97_06075"
     CDS             1764..2786
                     /locus_tag="VHS97_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013562973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3447800.1"
                     /translation="MKILVTGSEGFIGGYLVEELLANGYEVVGLDNFSKYGEIEHASR
                     HNPRYHLVRGDAKDVDLQKELLRDCDHMIAGAAMIGGISYFHSFAYDLLAENERITAA
                     AFDAAIWAHRQAKLKKITVISSSMVFENATHFPSSEGEERRCPPPGSTYGFQKLAVEY
                     YAQGAHEQYGLPYTIARPFNCVGIGERRARSDKEILSGNVRLAMSHVVPDLVQKVLKG
                     QDPLHILGDGQQVRHYTYGGDLARGIRLCVEHPAALDEDFNLSTETSTSVIELAELIW
                     RKIHGEWKPFRTISDDPFRYDVRCRIPSIEKAAKLLGFEANTGLDEILDEVIPWVEKQ
                     VEAGVI"
     gene            2786..3538
                     /locus_tag="VHS97_06080"
     CDS             2786..3538
                     /locus_tag="VHS97_06080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3447801.1"
                     /translation="MTTLAHAALVPQPADKLDSLALIVPVYNEAENFPALLAQIERFI
                     LEPFVLHVVYDFDEDTTVPVARAFAKERPWLRLVKNRYGRGVVAAIRTGFHEVRDGPA
                     LVIMADLSDDLTIVPQLMELYRRGNRIVCPSRYVRGGRQIGGPLVKRVLSRAAGLSLR
                     LLARFPTHDATNNFRLYDAALVNKLGIESTGGFELALEMTAKAHHQGIKIAEVPSTWR
                     DRTAGQSQFKLMKWLPKYLYWYAYALFGGRRR"
     gene            3581..4279
                     /locus_tag="VHS97_06085"
     CDS             3581..4279
                     /locus_tag="VHS97_06085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3447802.1"
                     /translation="METLQDLGKEYQRRFASLAPYRGAVWRVLVRDFFQRYVDPRATV
                     LDLGSGWGEFIGNIHAQRRIAMDLNPDMPSRVGSGVETILQDCSQPWQVADSSLDLVF
                     TSNFFEHLPDKDALRRTLHEALRCLKEGGRIICLGPNIRFLNGPYWDFWDHFLPLTDR
                     SMVEILTLTGFAVERIEPRFLPYSMSQGFTPPIGFISLYLRIPLLWRILGKQFLVVGR
                     KTFREPADPVSHHS"
     gene            complement(4306..6606)
                     /locus_tag="VHS97_06090"
     CDS             complement(4306..6606)
                     /locus_tag="VHS97_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX3447803.1"
                     /translation="MRASLRSGWIRRLCVVVVVGAAMESRSDAENLPIVLDVEYQPLV
                     AQVKRVIDALEMLGQPLDRDEKARLTAALDSTGGESSIRAIQNVLDVRALIGIEINPE
                     SRVKSSPGPARPRLVQNGWSVFLVKVDNQAGVTAELAAESPNAASLYKRSTGRAQPTA
                     TIRPTDVVQRWADISMFNDRPLKSSLSGLRLEYRIIQVYSRDSGKREASISFNVGQGT
                     QDLGFRSAVDILFTCDPAVSVVLDIRDSDGRPAMASFVFRDGRGRVYPATSRRLAPDF
                     FFHPQIYRQSGESVALPPGTYRVEFTRGPEYVTQSRTITIPQTRTHRESFLLRRWIQL
                     SKMNWFSGDHHVHAAGCAHYESPTEGVKPEDMWRQILGEDLDVGCVLSWGPCWYAQKQ
                     FFDGKIHPLSTSEYLMRYDVEVSGFPSSHAGHLCLLRLKEDDYPGTSRIEEWPSWDLP
                     VLKWGKSQGGVVGFSHSGWGLKLSDDKLPSYQVPPFDGIGANEYIVDVVHDAVDFIST
                     VDTPAPWELNIWYHTLNCGFRTRISGETDFPCIYGERVGLGRVYVRLDDGQLDFDKWC
                     EGIKLGRSYVGDGRSHLIDFQVNDLRMGESGGELKLDGAATVKVTAQVAAYLEPEPSA
                     EARAIRYRPLAEKPYWDLERAREGVTRKVPVEVVVNGESVANAAVSADGSIQGVSFDV
                     PIKKSSWIALRIYPSCHTNPIFVSVGGKPVRASRKSAEWCLKSVDQCWSKKEPAIRES
                     EKEEARKAYEIARDSYRKILAESDED"
     gene            7047..8315
                     /locus_tag="VHS97_06095"
     CDS             7047..8315
                     /locus_tag="VHS97_06095"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3447804.1"
                     /translation="MATTHSLDSIRSTQPVLYLAFELSSSQWKVASTTARGQKPRLVS
                     VPAGDRDEVLREVARAKKRFDLPRNATVVSCYEAGRDGFWLHRFLHQFGVNNLVVDSS
                     SIEVNRRLRRAKADNLDAISLVGLLIRYCEGDTKVWSVVTVPSAADEDHRHPHRELNQ
                     LRRERTTHTNRIRGLLTAIGIKITGDCSLSAGDLDSWRQWNGEPLGENLKHRLLRELE
                     RLELLTRQIRVIKAEQVARIRDDRTHHVAKVRRLMGLKGVGQTSATILVYEFFGWRQF
                     KNRREVAALAGLTPTPYQSGESNHEQGISKAGNVLIRWIMVELAWSWLRFQPDSPLSQ
                     WYHRRFGGGTARMRRTGIVALARKLLIALWRYLERGEALENAAEMNWEAKLRPPARRQ
                     RSQGSDQSPQVPPPDPHLLPSSLCSLGGRR"
     gene            complement(8903..10036)
                     /locus_tag="VHS97_06100"
     CDS             complement(8903..10036)
                     /locus_tag="VHS97_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447805.1"
                     /translation="MCLPRMVSRRWTLGLILVCVLFVLTMWTVRPEPVLPWFGMVGLG
                     NETPLTLLRIWAGGHSAPMTPVGLVSLFPAGELVLVISLIVTLLALLILFRAEWHDLG
                     TTSWSTQPIHPLRHLLGMKVRTLLVVIAVIGLELGWEIVTWRSWGVSEQYRARAGQYA
                     ENESRARESLRRVETMLAGRVEMGLGGPDETPAARSAEKAFRRDELGREHVYYSALID
                     YLGERKRVYEAAAEDTSLPIPPGPPRPGDRPGHELGMFLFQKEYAQALAHCAELIEHY
                     PDLVQVHERRAWILSTCPDANHRDGKLAVESATRAAELTNWKNPFVLSTLAAAYAEAG
                     NFASAVRWEEEVQRQYKSLGDRTSYPRERLALYKAGKPYRMGP"
BASE COUNT         2047 a         3024 c         2939 g         2097 t
ORIGIN      
        1 tgcgctcctc gatcaccccg ttggccgatt gcctctgcta tcgcattgcg tccattatga
       61 ttataccaaa ctccgacaga ggaaatcggc cgcactcatg acgcaagctg atggaccatc
      121 gctccatctc tctcactggt atttcgacgg ccacattgac gtggcccaca ggcgacgtat
      181 ctcgccaaat cgcaagctcg ctcgcatggt cggttgtatc tagcgatggc aaagctggac
      241 gtttgagtgt gaaatgtcga caacttaatg aggtcaaggg ggattcggcg cccgtaaacg
      301 atgaccaaga aagcgccggg ggccggcgga tggagcggag cgatcaaaag tcacaactcg
      361 gctggctgtc agggttctct cggctctcgg gactacacgt tcatggattg acagcccgat
      421 acgaccgtgg tatgcgacct gcccattgtt ggcgaattca tctcaagaaa ggatttctta
      481 agatggaatc ttctgtgtgc atcgtcggcg gctgtgggca cattggcctg cctctgggca
      541 tggccttggc cgaggtcggt gttccggtga ccttgttcga catcgacgtt gctcgcgtcc
      601 gccttgtcgc tgccggacaa atgcctttct tggaatgtgg cgccgaacgg attctgcccc
      661 atctgctgtc aaccgaccgc ctgaaagtca cgacttcgcc tgaggttatt cgccagcatg
      721 acatcgtggt cgtcacgatc ggaacacctg tcgacgaatt tatggacccc aatgtgcgat
      781 cgttcgatcg cgcaattgac cacgttttgg atcacatgag cgatggtcag ttattgattc
      841 ttcgaagcac ggtcttcccc ggcgtcacga ctcgcctcga tcggcgcgcc gcggagcggg
      901 gattgcgaat cgacatcgcg cactgcccgg agcggattgc ccagggatac gccttggagg
      961 aactgaccaa gttgccccag atcgtgggcg gtgtcacggc cgtagccagc cagcgttcgg
     1021 tggcgctgtt cagcctgctc ggaaccaagg tcatcaagct gccgccgatc gaagccgagc
     1081 tgaccaagct tttttctaac gcctaccggt acatcagctt cgccatctct aaccagttct
     1141 acgctatcgc cgagaatcta ggggccgact tcaagcgcgt ccggcaggcg atgatcgccg
     1201 attaccctcg catggctggg ttcccggcag cgggcctcgc gggcggaccg tgtttgctca
     1261 aggatacgat gcaactcgcg gccttcaatc ataacgcgtt tatcctcggc caggcagcca
     1321 tgatggtcaa cgaggggctt ccctcaatcc tggtcgaggc cgtcaaggcg aagtacagca
     1381 tgacggggtc gacggccgcc atcctcggca tggcattcaa aggtaacagc gatgatcccc
     1441 gcgattcgct ggcttacaag cttcgcaaag tcttgaccct tgaatgccgc cgcgtcctct
     1501 gctccgaccc ctatatcgac gaccccagct tcgtctccgt ggaaaccgcc ctcaaggagg
     1561 ccgatttcgt ttttctgggc gcttgccatg acgaataccg gaatctggtg atcgagaagc
     1621 cggtgatcga cgtcttcggc tttttgctcc agaacgtgaa gaccgctctc gagaaacgat
     1681 cggccgcttg agccaggcta tagtatctcc cgttatcgct tgctacctga tatctcaaga
     1741 tcccttcttt ctgaggattg ttcatgaaaa tcttggttac gggttcagag ggtttcatcg
     1801 gtggttatct cgtcgaggaa ctgctcgcga acggttatga ggtcgtcggc ctggacaatt
     1861 tttccaagta tggagagatc gagcacgcca gccgccataa cccgcgttac cacctcgtgc
     1921 gcggggatgc aaaggacgtc gatttgcaga aggaactgct gcgtgactgc gatcacatga
     1981 tcgccggagc cgccatgatc ggcggcattt cctatttcca cagctttgct tacgacctgc
     2041 tggccgagaa tgagcggatc acggcggcag cgtttgatgc ggccatctgg gcccatcgcc
     2101 aggcgaaact gaagaagatc acggtcatct cctcgtcgat ggtcttcgaa aatgccacgc
     2161 atttccccag ttcagaggga gaagagcgcc gctgcccacc gccagggtcc acctacggat
     2221 tccagaaact ggccgtggaa tactacgccc agggggccca cgaacagtat ggcctaccct
     2281 acacgatcgc ccggccgttc aattgcgtcg ggatcggcga gcgccgcgcc cgcagcgaca
     2341 aggagatcct ttccggtaac gtcagactcg ccatgagcca cgtcgtcccg gacctcgttc
     2401 aaaaggtgct caaggggcaa gatccgcttc acatcctggg cgacgggcag caggtgcgcc
     2461 actataccta cggcggcgac cttgctcgcg gaattcggct ttgcgtcgag caccccgccg
     2521 ctcttgacga agatttcaat ctctccaccg agacttcaac cagcgtcatc gagctcgctg
     2581 agttgatctg gcggaagatc cacggcgaat ggaagccgtt ccggaccatc tcggacgacc
     2641 cgttccgcta cgacgtgcgg tgccgcatcc cgtctatcga gaaagccgca aaactgctgg
     2701 ggttcgaggc caataccggc ctcgacgaga tactcgacga agtgattccc tgggtcgaaa
     2761 agcaggtgga agccggggtg atctgatgac gacgctcgcg catgccgcgc tggtcccgca
     2821 gccggccgac aaactcgatt ctctcgcgct gatcgtgccg gtttacaacg aggcggaaaa
     2881 cttccccgcc ctcttggccc agatcgagcg atttattctc gaaccgtttg tcctccacgt
     2941 cgtgtacgac tttgacgagg acaccacggt tccggtggcg cgtgcgttcg ccaaggaaag
     3001 gccttggctc cggctagtca aaaaccggta cggccgtggc gttgtcgcgg cgatccggac
     3061 cggctttcac gaggtgcgcg acggcccggc tctggtgatc atggccgatc tctccgacga
     3121 cctcaccatc gttccccagc ttatggagtt gtaccgccgg ggcaatcgca tcgtctgtcc
     3181 gagccgctac gtccgcggcg gccgccagat tggtggacca ctggtcaagc gtgtgttgag
     3241 ccgtgcggcc gggctatcgc tccggttgct ggccaggttc ccaacccacg acgcgaccaa
     3301 caatttccgc ctctacgacg cggcgctcgt taacaagctg gggatcgaaa gcactggcgg
     3361 attcgagctg gctctcgaga tgaccgccaa agcgcatcac cagggcataa agatcgccga
     3421 ggtgccgtcg acctggcgag atcggaccgc cggccaatcg cagttcaagc tcatgaaatg
     3481 gctgccgaaa tacctttact ggtacgccta cgccctgttc ggcgggcggc ggcgttgagt
     3541 tgacaggttt gcgacgacgc gacgtattgg gctaataatg atggaaacac tacaagacct
     3601 cggaaaagaa tatcagcgtc gattcgcttc attagctccc taccgtggcg ctgtctggcg
     3661 ggtcttggtg cgtgatttct ttcaacgtta cgtcgatccg cgcgctacgg ttctcgactt
     3721 gggaagcggc tggggcgagt ttatagggaa cattcatgct cagcgacgga tcgcgatgga
     3781 tctcaatcca gacatgccct cccgcgttgg ttcgggtgtg gaaacgattc ttcaggactg
     3841 ctcgcagccc tggcaggtcg ccgattcttc acttgacctt gtgttcacca gcaatttctt
     3901 tgaacatctt cctgacaagg atgcccttcg aaggacttta catgaagctc tgcgctgcct
     3961 gaaggaggga gggcgtatta tatgccttgg tccgaacatt cgatttttga acgggccgta
     4021 ctgggatttt tgggatcatt ttctgccgct aacggaccgc tcgatggtcg aaatcctcac
     4081 tctcaccgga ttcgccgtgg agcgtattga gccacgcttt cttccttatt ccatgtctca
     4141 gggtttcacc cctccgatcg ggtttatctc tctctacctc cgtattcctc ttctatggcg
     4201 cattctcggc aagcagtttc tggttgtcgg acgaaaaact ttccgcgaac ccgccgaccc
     4261 cgtttcacac cactcatagt gagtgatcgc cgcgctgctc ggctatcaat cttcgtcgct
     4321 ctctgccaag attttcctgt aagagtcgcg ggcgatttca taggctttgc gggcctcttc
     4381 cttttcactc tctcgaatgg cgggctcttt cttcgaccag cactggtcga ccgacttcag
     4441 gcaccactcg gccgattttc tcgaagcccg gaccggcttg ccgccaactg agacgaagat
     4501 ggggttggta tggcaggacg ggtagattcg cagcgcgatc cagctcgact tcttgattgg
     4561 cacatcaaac gacactccct ggatgctgcc atcggccgaa acggccgcat tggcgacgga
     4621 ctcgccgttg accacgacct cgacgggcac cttgcgagtc acgccctcgc gagcacgctc
     4681 caggtcccag tacggctttt ccgccagtgg ccggtaacga atggcgcggg cttccgctga
     4741 cggttccggt tcaagataag cggcgacctg ggccgttacc ttgacggtcg cagcaccatc
     4801 gagcttgagc tcgcccccgg attcgcccat acggagatcg ttcacctgaa agtcgatcaa
     4861 atggctgcgg ccatcaccga cataagaccg gcctagcttg atcccttcgc accatttatc
     4921 gaagtcgagc tggccgtcgt cgagtctgac gtagacccgc cccaggccga cgcgttcgcc
     4981 atagatgcac gggaagtcgg tctcgccgga gatccgggta cgaaatccac aattgagcgt
     5041 gtgataccaa atgttgagct cccagggcgc cggggtatcg accgtggaga taaagtcgac
     5101 cgcgtcgtgg accacatcga cgatgtactc gttggcgccg atgccgtcaa aaggagggac
     5161 ttgatacgac ggcagcttgt cgtccgacag tttcagcccc catcccgaat gcgagaaacc
     5221 gacgacgccc ccttgcgact tgccccactt gagcacaggt aggtcccagc ttggccactc
     5281 ttcgatccgc gacgtccccg ggtaatcgtc ttccttgagg cgcaacaagc ataagtggcc
     5341 cgcatgtgat gacggaaacc cggagacctc gacgtcgtat cgcatcaagt actcggaagt
     5401 cgagagcggg tggatcttgc catcgaaaaa ctgcttttgt gcataccagc atgggcccca
     5461 tgaaaggacg cagccgacat ccaggtcctc cccgaggatc tggcgccaca tgtcctctgg
     5521 cttgactccc tcggtcgggc tttcgtaatg cgcacacccg gccgcatgga cgtggtgatc
     5581 gccggaaaac cagttcatct tggacaattg gatccagcgc ctcagtaaaa acgactcccg
     5641 atgagtacga gtctggggga tcgtgatcgt ccgcgactgg gtcacgtatt cgggtccgcg
     5701 tgtgaactcg acgcgatacg tgcctggcgg cagagcgacc gactccccgc tctggcgata
     5761 gatctgagga tgaaagaaga aatccggggc aagtcgccga ctagtcgcgg gataaacccg
     5821 accccgaccg tcgcgaaaca cgaatgacgc catggccggc cggccatcgc tgtcgcggat
     5881 gtcgagaacc acgcttaccg ccgggtcgca agtgaagagg atatcgacgg cgctgcgaaa
     5941 acccaggtcc tgggttccct ggccgacatt aaagctgatc gatgcctcgc gcttgccgga
     6001 atcacggctg taaacctgga tgatccggta ttccagcctc aggcccgaga gcgacgactt
     6061 gagaggccgg tcattgaaca tggatatgtc ggcccagcgc tgtacgacgt ccgtggggcg
     6121 aattgttgcc gtgggttggg cgcggccggt tgaccgcttg tataaagagg ccgcgtttgg
     6181 gctttctgcg gcaagctcgg ccgtcacacc ggcttgattg tcaactttga cgagaaaaac
     6241 ggaccagccg ttctgaacca accgtggtcg cgccggccct ggcgacgatt tgacccggct
     6301 ctcggggttg atttcgatcc cgatcaaagc gcgcacgtcc aggacattct gtatcgcacg
     6361 aatcgacgac tcgccgccgg tggaatcaag cgctgccgtc agccgagcct tctcgtcgcg
     6421 atcgagcggc tgccctaaca tctcgagggc gtcgatcacg cgcttcacct gcgcgaccaa
     6481 gggctgatat tcgacgtcca gaacgatcgg caggttctcg gcatcgctac gggattccat
     6541 cgctgcgccg accacgacca ctacgcacag gcggcgtatc cagccagatc gcaagctcgc
     6601 tcgcatgttc ggttgttcct ctcgcatgtg gagctctgtc cgttagcctc aaaagtcgtc
     6661 acgtccacag gtttagcggt ttcgggtacg cggatcaatg accaagactg gatcggcgcc
     6721 tttcggggtc cgcaatggat ttgagatatg tggtgaaacg cagggctgca aaccgtcatc
     6781 tgagcgattc aaaaaagtcc gtgtatggct gccgcgcccg ttccatgtcc cacggccgcg
     6841 ctttggcctt ggccaggccg caatgtctgg catcgaatgc cggatcgtcc cagagccgct
     6901 cgatcacgtc tacccacggg gcgactccgg ggctgcaagg gggctgcaag atactatgtg
     6961 agcggtcagt aagcgatatg taatgcgagt cgttgcgagt aagctcgaat tggtgacatc
     7021 aacactcgtt ctcaaggagc ttacccatgg caacgactca cagcctcgat tctatccgat
     7081 ctacccaacc ggtgctctat ctcgcgttcg agctctccag cagtcagtgg aaggtcgcct
     7141 cgactaccgc acgtggtcaa aagcctcgtc ttgtctcggt gccggctggc gatagggacg
     7201 aagttctcag ggaggtcgct cgagcaaaga aacggttcga tctccccagg aacgcaacag
     7261 tagtctcgtg ctacgaggcc ggacgcgacg gcttctggct ccatcgcttc ttgcatcagt
     7321 tcggagtcaa caacctggtg gtcgattcgt cgagcatcga ggtcaatcgc cgcctccgac
     7381 gtgccaaggc agacaacttg gacgctatca gcttggtcgg actgctcatt cgctattgcg
     7441 aaggtgatac caaggtgtgg agtgtggtca ccgtgccgag tgccgctgat gaggaccacc
     7501 gtcatccgca ccgcgagctc aaccaacttc ggcgtgagcg tacgacccac accaatcgca
     7561 tccgcggtct cttgaccgcg attggtatca agatcaccgg cgactgctca ttgtcggccg
     7621 gcgacctcga ttcgtggagg cagtggaacg gtgagccgtt gggtgagaac ctcaagcacc
     7681 gcctgctgcg tgagctcgag cgcctggagc tgctcacgcg tcagattcga gtgattaagg
     7741 ccgagcaggt cgcgcggatc cgcgacgatc ggactcacca cgtggccaag gtgcggagac
     7801 tgatggggct aaaaggggtg ggccagactt cggccacgat cctggtgtac gagtttttcg
     7861 gctggcggca atttaaaaat cgtcgcgagg ttgccgcttt ggcggggctg acgccgacgc
     7921 cctaccagag cggcgagagt aaccacgagc agggcatcag caaggccggc aacgtcttga
     7981 tccgctggat catggtggag cttgcgtgga gctggttgcg tttccaaccg gacagcccat
     8041 tgagccagtg gtatcacagg cggttcggcg gcggcactgc ccgcatgaga cggacgggga
     8101 ttgtggcgtt ggccaggaag ctcctgattg cactctggcg gtacctcgag cgcggagaag
     8161 ctctggaaaa tgccgcagag atgaactggg aggcaaaact gaggcccccg gcacgtcgcc
     8221 agcgtagcca ggggagtgac cagagtcccc aggtccctcc cccggacccc cacctccttc
     8281 cctcttctct gtgttccctc gggggaagac ggtgatgacg tgcttctgta tgttcataat
     8341 acaaatcatt gattacaggt tgtgagggcg agtcatgggc gaacagggct ggagaagcga
     8401 ccgagcaagt gtcctcggct accactttag tggggccgtc tgcaagatgg gtcgcttctt
     8461 cagttctggc ttcgaggcgt cgcgagacgc atacaacatc tggtgatcag ttaactatat
     8521 gactgcacac ggatagaagg ctgctgcgac ccgactgggc gcacacccga ggaagccacc
     8581 cagccctgaa cttttcatga cgcccggacc aacgaaaggg aagcaagctc ggtcactgta
     8641 ttctgatctg gtcgtcgttc gcgatccgcg gcgtttgtga atgagctgaa acggggtcga
     8701 cagcccttca cgcaggctca ggccgatcgc ggttcgtcgt caacctgggg gaactcacgc
     8761 tatcacagaa aaatcggcgg cagggcttga ctcgcgcggc tccatagaag gacacgcgtt
     8821 gtcgcccgag gagaaacagt acgtcatcga cgtgatccgc cgctggctcg acttgcagac
     8881 tcgaaagtga attccggccg cgctacgggc ccatgcgata gggtttcccg gccttgtaca
     8941 acgccagccg ctctcgaggg tacgaagttc gatctcccaa ggatttgtat tgcctttgga
     9001 cctcttcctc ccagcgaact gccgatgcga aattgccagc ttcggcgtaa gcggccgcga
     9061 gagtcgagag cacaaacggg tttttccagt tggtcaactc tgccgctcgt gtggccgact
     9121 ccacggcgag ctttccatca cggtgatttg cgtccggaca ggttgacagg atccaggctc
     9181 ggcgctcgtg gacctggaca agatcggggt aatgctcaat gagctccgca cagtgggcaa
     9241 gagcctgggc atactctttt tgaaacagga acatgccaag ttcatgtccg ggtcggtctc
     9301 ccggccgagg cgggccgggg gggatcggca gcgaggtgtc ctcggcagcg gcttcgtaaa
     9361 cccgcttgcg ctcgccaagg tagtcgatca atgcagagta gtagacatgc tcacgtccga
     9421 gttcgtcgcg tcgaaaggct ttctcggcgg accgggccgc gggagtctca tcaggccctc
     9481 ccaagcccat ttccacgcga ccggcaagca tcgtctcgac gcgtcgcaac gactctctcg
     9541 ccctggactc gttttcggca tattgacccg ctcgtgcgcg gtactgctca ctcactcccc
     9601 agcttctcca ggtaacaatc tcccacccga gctcgagacc gatgacggcg ataacaacga
     9661 gcaacgttcg gaccttcatt cccagtaaat ggcgaagcgg atggattggc tgggttgacc
     9721 acgacgtcgt tcctagatcg tgccactccg cacggaacag gatcagtaag gccaacagag
     9781 taacaatcaa actaatgaca aggacgagct cgccggctgg aaagagcgag accagcccga
     9841 ctggcgtcat cggagccgaa tggccgccgg cccaaattct cagaagagtg agtggcgtct
     9901 cgttacccag ccccaccatg ccaaaccatg gcaggaccgg ttccggccga actgtccaca
     9961 tagtgagaac gaagagaacg cacaccaaga tcaggccgag ggtccaacgc cttgacacca
    10021 tccggggcag acacatggtt catatcccca taaccgatta gcacggcatc ggagaatcga
    10081 cctgatcgca catgccggat tgagagc
//
LOCUS       DATAQL010000353         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2935688, whole genome shotgun sequence.
ACCESSION   DATAQL010000353 DATAQL010000000
VERSION     DATAQL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2935688"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VHS97_06105"
     CDS             complement(<1..433)
                     /locus_tag="VHS97_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEX3447806.1"
                     /translation="MAKRQGTTESKPSATQEQKALNNALSQIEKTFGSGAIMKLGEGS
                     HMEIEGISTGALSLDLALGGKGLPRGRMVELFGPESSGKTTIALHAVAQAQQKGGVAA
                     FIDVEHALDPAWCKRLGVDIEALLVSQPGSAEEALQIAEMLV"
     gene            complement(519..1106)
                     /gene="thpR"
                     /locus_tag="VHS97_06110"
     CDS             complement(519..1106)
                     /gene="thpR"
                     /locus_tag="VHS97_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX3447807.1"
                     /translation="MPERTRTFIAIAVPEPVEQKLTQLQAELAPEIAGFRWNSTRPFH
                     ITLAFLGDVPDGDLNKICQAVAASTVSFHPLKIEVRGLGAFPNATRPRIVWAGTTATN
                     INALIDLRQSIVGQLKRIGHDPDEERFHPHVTIGRIKHDRRDSRGLHTLIEARAGWSA
                     GEFEVAEVLVMASTLGRAGPVYEVLGRGDLGGKKP"
     gene            complement(1147..1605)
                     /locus_tag="VHS97_06115"
     CDS             complement(1147..1605)
                     /locus_tag="VHS97_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447808.1"
                     /translation="MARRKWIILGGVFLLLLTVGAELVVRPWNPSKACVQIVNQGDAA
                     CDDLVVVYGATKVRLGRLGSGETTSAWFSAAEKGPVTLEFNQHGNPMKGFQIPDFDPI
                     DNISNGFKLVLVLKKDQVERFMDDDPTRTPLKTLMDNVKAWFESEIGFPL"
     gene            complement(1729..2706)
                     /locus_tag="VHS97_06120"
     CDS             complement(1729..2706)
                     /locus_tag="VHS97_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX3447809.1"
                     /translation="MRIFVTGSIAYDYIMVFPGRFRDHILPDKMHVLSVSFLVDSLTR
                     LRGGTGANISFNLGLLGEHPFLVGTVGEDFAEYRAWLESHGVDCGTVKIITGDHTASC
                     FINTDLQDNQITAFYPGAMAHAASLSIVEAGVEPSDLVVIAPNDPGAMNRYAAECVAA
                     KIPYLYDPSMQLPRLEREELEKGCRGARIVAGNDYEFGMMAEKLGVTEAELRRLAPIT
                     VMTKGEAGALITVGPDEFEIPPAKPKEVVDPTGAGDAFRAGFILGIKQKLPWPVVGRL
                     AALTAVYAIENKGPQQHSYTHDQFVARYRESFGSDPGIAKPANLATAGR"
     gene            complement(2785..>3280)
                     /locus_tag="VHS97_06125"
     CDS             complement(2785..>3280)
                     /locus_tag="VHS97_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX3447810.1"
                     /translation="EDKVEGVLPFLKKEALVSDPMSPSERMRIAAVITALGDRLKVFA
                     DILKLGRFFFTESLAYDPDAVKKRLRKVGVAAMLEELDLVLAQVEPFDLASLESAVHA
                     YAERTGRKMGDVVNPLRVAVTGQGIGPGLYDCLAIVGRDLCRARVRQTVEMLQTGESL
                     PKPG"
BASE COUNT          662 a          989 c          943 g          686 t
ORIGIN      
        1 ggaccagcat ctcggcaatc tggagtgcct cttccgcact accgggctgg ctgaccagga
       61 gggcctcgat atccacacca agtcgcttac accaggccgg atccagcgca tgctcgacgt
      121 cgatgaaggc cgcaacgcct cctttttgct gggcctgagc gactgcatgc agcgcgattg
      181 tcgttttgcc gctcgactct gggccgaata actcgaccat gcgaccgcgc ggcaaaccct
      241 tgcctcccag cgccaggtcc agcgacaagg cccccgtcga gatcccctca atctccatgt
      301 gggacccctc acccaacttc atgatggccc ctgacccgaa tgttttctca atctggctga
      361 gcgcgttatt gagcgccttc tgctcctgag tggcactcgg cttgctctcc gtcgtcccct
      421 gtcgtttcgc cacgaccatc gctccttccg acacccgcca ctttatgtac gaaattacag
      481 tactctaaca ttcctgatcg gcattggtca aggacgacct acggtttttt tcctccgagg
      541 tcaccgcgac cgagcacctc ataaacgggg ccggctcggc ctaaagttga ggccattact
      601 aacacttctg cgacctcgaa ttctcctgcc gaccagccag ccctggcttc aatcaaggtg
      661 tgcaggccac gactgtcacg acgatcgtgc ttgatgcggc caatggtcac gtgaggatga
      721 aaccgttcct cgtcgggatc gtggccgatc cgctttaact ggcccacgat tgattgtcgc
      781 aaatcaatga gggcattgat gttcgttgcc gttgtgccag cccagacaat ccggggacga
      841 gtcgcattgg gaaatgcacc taaaccgcgc acttcgattt taagcggatg gaaagaaaca
      901 gtgcttgcgg ctaccgcctg gcagatcttg ttcaggtcgc cgtctgggac gtcgccaagg
      961 aaggcaagtg tgatgtggaa cggccgggtc gaattccagc ggaatccggc gatctctggc
     1021 gcgagctcgg cttggagctg cgtgagcttt tgctcgacgg gctcggggac ggcaatggcg
     1081 ataaaagtcc gcgtgcgttc aggcatgacc gaatcatcgc aactgcgtca aaacgttccc
     1141 tcgcgttcag agcggaaaac cgatttcgga ctcgaaccaa gccttgacgt tatccattaa
     1201 ggttttgagc ggggtcctgg tcggatcgtc gtccatgaaa cgctcgacct gatccttctt
     1261 tagcaccaat acaagtttga agccgttgct aatgttatcg atgggatcga agtccgggat
     1321 ctgaaaaccc ttcatcgggt ttccgtgctg gttgaactca agggtaacgg gacctttctc
     1381 ggcggcggaa aaccaggcgc tcgtcgtctc gccggaaccg agccgtccca acctgacttt
     1441 ggttgctccg tagaccacga ccagatcgtc gcacgccgcg tccccctggt tgacgatttg
     1501 tacgcacgcc ttggacggat tccaaggccg gacgaccaac tctgccccga ccgtcagaag
     1561 caggagaaat actccgccca gaatgatcca ttttcgccga gccattggga gcgtagatac
     1621 ctgcgtctct gggtccgatc ggtcttgggt cttggctcag accgaagtat tgccacgaaa
     1681 ataggattac gcgcgccggc ggttaccaca agcagaaacg gaatcctcct accgtccggc
     1741 tgtcgccaga ttggccggct tggcaatgcc gggatcagag ccaaaactct cgcggtagcg
     1801 cgcgacaaac tggtcgtgcg tgtacgagtg ttgctgaggg cccttgttct cgatagcgta
     1861 aacggcggtg agcgccgcga gccggccaac cacgggccac ggcagttttt gcttgatacc
     1921 gaggatgaaa cccgcgcgaa aggcatcgcc ggcacccgtc ggatcgacca cttccttggg
     1981 tttggctggc gggatttcaa actcatccgg gccgacggtg atcaaggcac ctgcttcacc
     2041 cttggtcatc acagtgatgg gcgccagccg acggagctcg gcttcggtca cgcctagctt
     2101 ttcggccatc atcccgaact cgtagtcgtt gccggcgacg atgcgtgcgc cgcggcaacc
     2161 tttctcgagc tcttcgcgct cgaggcgggg caattgcatt gaggggtcgt agagataggg
     2221 aattttggcg gcgacgcact cggctgcgta ccggttcatc gcgccggggt cgttgggcgc
     2281 gatcacgacg agatcgctgg gttcgactcc cgcctccacg attgagagtg acgcggcatg
     2341 cgccatcgcg cccggataaa atgcagtaat ctggttgtcc tgcaggtcgg tattgatgaa
     2401 acaggaggct gtatggtccc ctgtgatgat cttgaccgtc ccgcagtcga caccatggct
     2461 ctccaaccag gcgcggtatt cggcgaagtc ttcaccgact gtgccgacaa ggaagggatg
     2521 ttcgcccagg aggccgagat taaaggagat gttggcccct gtgcctcccc gtagtcgggt
     2581 cagtgagtcg accaggaacg acacgctcaa aacatgcatc ttgtcgggca agatatggtc
     2641 acgaaaccgg cccggaaaca ccatgatgta gtcgtaagcg atcgagccag tgacgaaaat
     2701 gcgcattcgg cgtgtccttg taacgatgag aacaaaggcc ctggcgcggg ccgatctagg
     2761 gtcgtcgagc gaattcagtc atcttcaacc cggctttggg agggattcac cggtttgcaa
     2821 catttcgaca gtctggcgaa cgcgggcccg gcaaaggtcg cgccccacga tcgccaagca
     2881 gtcgtaaagc cctggcccga tgccttgacc cgtgacggcc acgcgcaatg ggttcacgac
     2941 gtctcccatc ttgcggcccg tgcgctcggc gtaggcgtgt acggccgatt cgagggacgc
     3001 cagatcgaag ggctcgacct gagccaaaac caaatcgagc tcctccagca tcgccgcgac
     3061 tccgactttt cgtaaccgtt ttttgacggc atcggggtcg tacgccagcg attctgtgaa
     3121 gaagaaccgg cccagcttga ggatatcagc aaagactttg agccgatcgc ccagcgccgt
     3181 gatcacggcc gcgatccgca tgcgttcgga tggcgacatg ggatcgctga ccaaggcctc
     3241 tttcttcagg aacggcagca caccttccac tttatcttcc
//
LOCUS       DATAQL010000354         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3699625, whole genome shotgun sequence.
ACCESSION   DATAQL010000354 DATAQL010000000
VERSION     DATAQL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3699625"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VHS97_06130"
     CDS             <1..245
                     /locus_tag="VHS97_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447811.1"
                     /translation="LGDQVLLTGADALQGQYQKPQGFSVLLHVDAAADAERIFKMFAE
                     NGAVQMPVQETFWALRFGMLIDQFGTPWMINCGRPA"
     gene            complement(384..518)
                     /locus_tag="VHS97_06135"
     CDS             complement(384..518)
                     /locus_tag="VHS97_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447812.1"
                     /translation="MPSDVGATIYRALGAKPAREVCDQPVQLNRGVVISPLFDGRTTA
                     "
     gene            complement(536..1225)
                     /locus_tag="VHS97_06140"
     CDS             complement(536..1225)
                     /locus_tag="VHS97_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX3447813.1"
                     /translation="MSEATMRSKPAARASASRVVTSVPSAGSDYLPEHRSVESLRVAE
                     QSCRGCELYQRATQAVGGEGPTTARMVLVGEAPGDEEDKIGRPFVGPAGILLDQVLEA
                     VGIDRTDVFVTNVVKHFKWEPRGKRRLHAKPNSQEIAACRAWLDAELALVRPRVIVCL
                     GATAAQALLGRNFRITQRRGEVVSGSLGNVVATYHPSAVLRAPDADARQKLRQALIDD
                     LRVAARLIGTG"
     gene            1506..>1823
                     /locus_tag="VHS97_06145"
     CDS             1506..>1823
                     /locus_tag="VHS97_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447814.1"
                     /translation="MPKSVTRIMLLLACMCTTALILLAVAGCSATEKPQLGTNHPEVT
                     VIVARRRTVPHVVRPIGTTRALQEVAIRARVKGFLEQKHFEDGKNVKKGQLLLVIEEK
                     PYQV"
BASE COUNT          355 a          576 c          534 g          358 t
ORIGIN      
        1 ccctgggcga ccaggtattg ctgacgggcg ccgacgcgct ccagggacaa tatcagaaac
       61 cacagggctt ctctgtattg ctccacgttg acgctgcggc agacgcggaa cgcatcttca
      121 agatgtttgc cgaaaacggc gcggtgcaaa tgccggtcca ggaaaccttc tgggcattgc
      181 gttttggcat gctcatcgac caattcggca caccgtggat gatcaattgc ggaagaccgg
      241 catgaagctt tgcccgacgt gcgattttga aaaacagcgt gctcgccggc ctgttcgttg
      301 gcgacggtaa gtttgtcagt cgctgccaca aattgccagt gatcaaacgg ccgcgcggca
      361 acttctgact tcatcgactc ttttcaggcg gtcgttctcc catcgaacag cggggagatg
      421 accactcccc ggttgagctg gacgggctgg tcgcaaacct cgcgggccgg cttggctccg
      481 agtgcgcgat aaatcgtcgc acccacgtcg ctgggcatag atcacggatt tgacgtcagc
      541 ccgttccgat gagccgcgct gccacacgga ggtcatcgat tagggcctgc cttaacttct
      601 gccgcgcatc ggcatcaggg gctcgcagca cagccgacgg atgatacgtg gccacgacat
      661 tccccagaga tccactgacc acctccccgc gccgctgggt gatgcggaag ttgcgaccca
      721 gcagggcctg ggcggcggtt gcgcccaggc agacgatgac ccgcggccgg accaatgcga
      781 gctcggcgtc gagccacgct cgacatgcgg cgatctcctg agagttgggc ttggcatgca
      841 atcgtcgctt gccccgcggt tcccatttga agtgcttgac tacattcgtg acgaagacgt
      901 cggtccgatc gataccgacc gcctccagca cttgatcgag caaaatcccc gcgggaccga
      961 cgaacggccg gcctattttg tcctcctcat ccccgggcgc ttccccgacc aggaccatac
     1021 gagcggtcgt cggtccttca cctcccacgg cctgggttgc ccgctgatag agctcgcaac
     1081 ctcgacacga ttgctcggca actcgtagtg attccaccga acgatgttcc ggcaaataat
     1141 cactgccggc agagggaaca gatgtcacga ctcgagaggc actcgctcgc gcggccggtt
     1201 tcgatcgcat tgttgcctcc gaaatgacgc tcgagtttca cgcggccatc catcacttgg
     1261 cgggtcgcgt tccggtcgtc gaggacattt ccgcactctt catgccggaa tcccctttca
     1321 ctggtcgtta ttgtactaac cggtctcagc atcgacgaag ccgccctacc ccttggactc
     1381 tcggaccctc gacgatccct tcccgagtgc gccgcggaag gcgcactgga ttccccggac
     1441 gggcaccgct agagtatcgc tgcgattgag agtcacgctc gatggtgatg cccgtgggtg
     1501 caacgatgcc gaaatcggtc acaagaatca tgctccttct ggcctgcatg tgcacgacgg
     1561 ccttgatctt gctcgccgtc gcgggctgca gcgccaccga aaagccgcag cttggtacca
     1621 accatcccga ggtcacggtg atcgtggcac ggcggaggac cgttccgcac gtggttcgac
     1681 cgatcggcac gacacgggcg ctccaggaag tggcgattcg cgctcgagtc aagggcttcc
     1741 tcgagcaaaa gcatttcgag gatggcaaga acgtcaaaaa ggggcaactt ctgctggtca
     1801 tcgaagagaa gccgtatcag gtg
//
LOCUS       DATAQL010000355         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4157187, whole genome shotgun sequence.
ACCESSION   DATAQL010000355 DATAQL010000000
VERSION     DATAQL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4157187"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(585..908)
                     /locus_tag="VHS97_06150"
     CDS             complement(585..908)
                     /locus_tag="VHS97_06150"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3447815.1"
                     /translation="MMAHEKSSSNVFTDLGLPNSEQELLKAKLTVQIYKLIKEQNLTQ
                     TKAAELLGTTQAQMSALMRCRPVSVSVGRLMEFLTILGQDVEVTVKPTRTRSTGHMSV
                     VVQPG"
     gene            complement(911..1282)
                     /locus_tag="VHS97_06155"
     CDS             complement(911..1282)
                     /locus_tag="VHS97_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX3447816.1"
                     /translation="MDELEPKQLVWVGSSRRDLREFPPAVRRTFGVALFAAQLGETSP
                     DAKPLRGFGGAGVLELIEDHRGDTYRAVYTVRFATKIYVLHAFQKKSKRGIATPKRDL
                     ELIRERLKWAERLHTGKVKEG"
BASE COUNT          327 a          439 c          460 g          316 t
ORIGIN      
        1 tcacccacac cccccaaacg gcagagtcgg tgagcctcgg ccgcgagggg gtttggaaca
       61 ccacccccga caacatcgcc gagtatgcat cgccaacgac caggtgaagt cctgtatcgc
      121 cgaactcaaa gcccaggcat agcgtcatcc ttctaaccac accgttcgat gccgactggc
      181 ttgaggggtg agcagcgcga gatgccggca ggcatcgagg cctcggcgcg ggtccaggga
      241 gatggaccga gcgcccggct tgggaaattt gccggcccac ttacaaacca gaaagccaga
      301 tgacgatgac aacgcgcctc agggacatca caagggtgct tcgggacacg gctccatctt
      361 gcctgtttgc agccgtgagg ggatgccgcg gggcgtgagg atggatctgt tcagcatgtt
      421 gaccgggcga aggctggcaa acgaggaaag caaggcccgc aagatcacga ctattgggga
      481 taagcatcca tgatcgcgtg gcccttaaac ggattaaaca tagtcaataa atgcactgct
      541 gttgtgtcca gcgatgtgat cttgccattc tcggagaaac tcgattagcc cggctgaaca
      601 actaccgaca tgtgtcccgt ggagcgcgtc cgcgtcggtt tgaccgtcac ttcgacgtct
      661 tggccgagaa tggtcagaaa ttccatgagc cgccccacag agacagatac ggggcggcag
      721 cgcatgaggg cggacatttg agcctgtgtg gtgccaagga gttcggcggc cttcgtctgc
      781 gttaggtttt gctccttaat gagcttgtag atctgtacgg tgagcttggc tttaaggagc
      841 tcctgttcgg aattcggcaa gccgagatca gtaaagacat tgctgctgct tttttcgtgc
      901 gccatcatgc tcagccctcc ttgactttgc cggtgtgcaa tcgctcggcc catttcaggc
      961 gttcgcggat gagctcgagg tcgcgcttgg gtgtcgcaat gccgcgtttc gatttctttt
     1021 gaaacgcgtg cagcacatag atcttggtcg caaatcgcac cgtataaacc gcccgatagg
     1081 tgtcgccgcg atgatcctcg atcaactcaa gcacgcccgc cccgccaaag cctctgagcg
     1141 gtttcgcgtc gggagacgtt tcgccaagct gtgccgcaaa cagcgcgaca ccgaaagtgc
     1201 ggcgcacggc cggcggaaat tcccgcaggt cccgacggct cgatccgacc cagacgagct
     1261 gtttcggctc gagttcatcc atagggccct atttatacat ttatatgtat aattgtcaag
     1321 tcccctgcgt caatcgatag tgtaacataa aggacgttat gttagactaa cgcgatccgg
     1381 ggggagggtc cgatgcaggg gaatcaaatt cagctttcgg gtgtcgccgt gacacccgag
     1441 cggcgcaacg tgccggccgt ggtcgagacc gcagcgccgg tgacgacggc gtcgctacac
     1501 agttcgctcg cggtcccgtc gatgattgcc agtgccggcg aa
//
LOCUS       DATAQL010000356         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1867264, whole genome shotgun sequence.
ACCESSION   DATAQL010000356 DATAQL010000000
VERSION     DATAQL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867264"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..383
                     /locus_tag="VHS97_06160"
     CDS             198..383
                     /locus_tag="VHS97_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447817.1"
                     /translation="MSNKEIVIEAIRQLPEQVSFEEIAEEIAILAAIQKGERDADAGR
                     MIPHDEVKKRLSSWISK"
     gene            732..1997
                     /locus_tag="VHS97_06165"
     CDS             732..1997
                     /locus_tag="VHS97_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEX3447818.1"
                     /translation="MLTGALVRVKIAKQRVVPLYIDREAGRWLEAAESLLLLFREGIG
                     MTRGEIEAEIDALFGGGGKATQVYRGLAKILEDRAEFEVVADVPPEVIREKVFTAAAD
                     YRRQLRSKLTGPVQRNAFRRDEILNAVARDLNMEPDAVATSLFADLRDEIRLLSFTDM
                     NAQWLIDRYNVGLAQAVLLRSVLVKVEVRNERPARYRQLFRRLKFHRLLYKVEGSMRD
                     GYVFHMDGPLSLFSATTKYGLQVALFLPALLHCSNFRLEAELRWGPRREPRSFLLEPR
                     DGLVTHQGDTGTYTPVELKAFVERFHQVATDWVISECTDVIELGREGVWVPDYRLVHT
                     PTGNDVFVEVLGFWKRSSVERLTRLLPRYGPPRFVLAISDRLKVDEEAFQELAVPILR
                     FKEIPNATELVALLDRFIGPAEPDQLPLT"
     gene            2039..>2411
                     /locus_tag="VHS97_06170"
     CDS             2039..>2411
                     /locus_tag="VHS97_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX3447819.1"
                     /translation="MGYRHRRSRAWLLFCAMLAMVSFTKASEEKETIAGLGQPVEILR
                     DRWGISHIYASNEHDLFLAQGFSAARDRLFQLELWRRQATGTLAEIEGPRALAADVGA
                     RLLRFRGDMRRELNHYHPRGYE"
BASE COUNT          511 a          704 c          705 g          491 t
ORIGIN      
        1 tccagcggct cgggcgcatc ctgcgcaagt cgggcgataa ggaagccatc ctctacgaag
       61 tcatcactcg cggaaccgcc gaagagttta cctccaaccg acggcgccga cacaatgcgt
      121 atgacggcga ttgaggcaag atgacgagcg ccgtacaatc cttgatgaaa acagtgtcac
      181 gatgagggga tttaaccatg tcaaacaagg aaatcgtcat cgaggcaatc cgtcaattgc
      241 ccgagcaagt gagtttcgag gaaatcgcag aggaaatcgc cattctggcg gcgatccaaa
      301 aaggtgagcg cgacgccgac gccggccgaa tgatcccgca cgacgaagta aagaagagac
      361 tcagctcgtg gatttcgaaa taacctggac cgaatcggca tcggccgatc tcgagtcgat
      421 tgtcaactac ctcgcccgaa taagcccaga cgtggcggaa aaaaccgcga acgcagtact
      481 taatcatata gaggtattgc ggacttttcc gcgcattgga cctcgttacg ccaaggatcc
      541 acaccgacaa gttcgagaag tcctctgcgg aaaataccga attttttatc ggctggatga
      601 gccacgcaaa ctggtcgaga tcctgacggt ttggcacggg gcgagggaag aacctcagct
      661 ttcctgacag tgtactgact cggcttatat cgctcatggc aagtgcggcc tgatcaagat
      721 ggaattgcct gatgctcacc ggcgccttgg tacgagtcaa aatcgcgaag caacgcgtag
      781 ttccgcttta tatcgaccgc gaggctgggc ggtggctcga ggcggccgag agcctgttgc
      841 tcttgtttcg cgagggaatc ggcatgactc gcggagagat cgaagccgag atcgacgccc
      901 ttttcggtgg tggcggcaag gcgacccagg tttatcgcgg tctggccaag atactcgagg
      961 atcgggccga gtttgaggtg gtggccgacg tgcctcccga ggtgattcgc gaaaaggtct
     1021 tcacggccgc agccgattat cgccgccaac tccgatctaa attgacgggc ccggtccagc
     1081 gcaacgcctt tcgtcgcgac gaaatcttga acgccgttgc ccgcgacctg aacatggagc
     1141 cggacgccgt cgcgacttct ctcttcgccg acctgcgcga cgaaattcgg ctgttatcgt
     1201 tcaccgatat gaatgcccag tggctcatcg atcgctataa cgtcgggctg gcccaggccg
     1261 tcctcttgcg ctcggtgctg gtcaaggtcg aggttcgcaa tgagcggccc gcgcggtatc
     1321 gtcagctctt caggcgactc aagtttcatc gcttgctcta caaggtcgaa ggcagtatgc
     1381 gcgacggcta tgtctttcac atggatggac ccttgagcct ttttagcgcc acgaccaaat
     1441 acggcctcca ggtcgcgctc ttcctgccgg cgcttttgca ttgctcgaat ttccggctcg
     1501 aagccgagct gcgctgggga ccaaggcgcg aaccacgcag ctttctccta gagccgcgcg
     1561 acggattggt cacgcatcaa ggcgacaccg gcacttacac cccggtcgag ctcaaggcgt
     1621 ttgtcgagcg attccaccag gtcgcaaccg actgggtgat aagcgagtgc accgatgtca
     1681 ttgaactggg acgtgaagga gtgtgggtcc cagactaccg gttggtgcac acaccaaccg
     1741 gtaatgatgt gttcgtcgag gtcttgggat tctggaagcg ctcaagcgtc gaacggctga
     1801 cacgtcttct ccctcgctac ggcccgcccc gcttcgtact ggccatctcc gatcggctca
     1861 aggtcgacga ggaagcattc caggagctcg ccgtgccgat cctccgcttc aaagagatcc
     1921 ccaatgcgac cgagctggtt gccctcctgg atcgattcat cggtccggcc gagcccgacc
     1981 aactgccact cacctgatct tcatcaacgt atttcccgct gatcgaaagg acgttcccat
     2041 gggctaccgg caccggcgat ctcgtgcgtg gctcttgttc tgcgccatgc tagccatggt
     2101 ctctttcacc aaggcgtccg aagagaaaga gacgatcgcc gggctcgggc agcccgtcga
     2161 aattctccgt gatcgctggg gaatctcgca catctatgcc agtaacgaac atgacttgtt
     2221 cctggcccaa ggtttcagcg ccgcgcgcga tcggctcttt cagcttgagt tatggcgccg
     2281 tcaggccacg gggaccctgg cggagattga ggggcccaga gctttagcag ccgatgtcgg
     2341 ggctcgattg ctgcggttca ggggcgacat gaggcgcgag ctgaaccact atcatccgcg
     2401 gggttacgag a
//
LOCUS       DATAQL010000357         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2783664, whole genome shotgun sequence.
ACCESSION   DATAQL010000357 DATAQL010000000
VERSION     DATAQL010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783664"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VHS97_06175"
     CDS             <1..443
                     /locus_tag="VHS97_06175"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEX3447820.1"
                     /translation="QSPIEYTVGAVRALDMLDPPPSTLVLADWAGRLGQDLFNPPNVG
                     GWPGGRSWLSARALIGRVNFATALVEGHGVGREIPFDPIALAGRYRRRGDRYETILFC
                     GELLLGFKPDRRCIDQPVSKITWNPESARQTIAQLMAAPEGQLC"
     gene            483..1727
                     /locus_tag="VHS97_06180"
     CDS             483..1727
                     /locus_tag="VHS97_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447821.1"
                     /translation="MITRRRMLRNSLKSATLVAMGPTVPTFLAHTAHAAAPRRDGRML
                     VVIQLDGGNDAINTLVPFGDEGYARNRKALRLPEKELIRVDDRVGLHPALTDLGKLLE
                     KGHLALVPGVGYPNPNRSHFESMSIWQTARLDLEEHGGPGWIGRSFDSLATTGDDNGA
                     AGTASLFVGAELTPAALRGRRAAASSLERIEDLTLPKGWDSSTPTIPLGVNPEGDAGL
                     SAYVRRSVVEAYSCSRKVAELTGRSAAATLGYPDTGLASKLRTIAQLLKVDLGARVYY
                     TTQPGYDTHAGQSNTHYGLLRELSGAIKAFLDDLTAANLAERVTVLCFSEFGRRVAEN
                     SSAGTDHGTAGLVLLAGPRVRPGVHGGVPSLTDLVDGDPKISTDFRTVYAALLEDWLG
                     LPSQTALGTRFEPMPLFWLTRA"
     gene            complement(1974..2585)
                     /locus_tag="VHS97_06185"
     CDS             complement(1974..2585)
                     /locus_tag="VHS97_06185"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX3447822.1"
                     /translation="MTRGKRTKGAGAAAPEQGRKGAATREAILQSARIAFTRSGYDGV
                     GVREIAQGAGVTAMMVNRYFGSKEQLFAEVVDNMQSTKSILTGHAGTLGQEAAAALAA
                     TPARGLDGFLLLLRSSSNPQAVTIIHDAIERNFQAPLAAMLPGANAGERAAVLLSIIA
                     GIQLMRQVFRLDALALESPEAQSVLARELEALFDQLIRRSDHR"
BASE COUNT          472 a          813 c          827 g          530 t
ORIGIN      
        1 tgcaatctcc catcgaatac accgtaggcg cggtgcgcgc gctggacatg ctcgacccgc
       61 cgccgagcac tttggttctg gccgactggg cgggtcggct gggccaagac ctctttaatc
      121 cgccgaacgt tggcggctgg cccggcggac gaagctggct ttcggcccgc gccttgatcg
      181 ggcgggtcaa cttcgcgaca gcactggtcg aaggacacgg cgtaggacgc gagataccct
      241 tcgacccgat cgcgctggcc ggccgatatc gccgtcgtgg agatcgatac gaaaccattt
      301 tgttttgtgg tgaattgcta ctcgggttca aacccgatcg gcgatgtatc gaccagcccg
      361 tctcgaaaat aacgtggaac ccggaatcgg cgcgccaaac gatcgcgcag ctcatggccg
      421 cgcctgaggg acagctttgt tgaacgcacg gaccgtctcg caccaagaga cttggaggct
      481 tgatgattac gcgacgtcga atgcttagaa attcactcaa gagtgcgacc cttgtggcca
      541 tggggccgac cgtgccgacg tttctggctc atacggccca cgcggccgcg ccccggcggg
      601 atgggcgcat gctggttgtg atccaactcg atggcggcaa cgacgcgatc aacactttgg
      661 tgccgtttgg cgacgaaggt tacgcacgga atcgaaaggc gcttaggctg ccggaaaagg
      721 aactgattcg cgtggacgat cgcgtcggcc tgcaccccgc cctgaccgac ctaggcaagc
      781 tcttggagaa gggtcatctc gcactggtac cgggcgttgg atatcccaac cccaatcgct
      841 cgcacttcga gagcatgtcg atctggcaga cggcgcggct cgacctggaa gagcacggcg
      901 gtccgggctg gatcggccgg agtttcgact cgctcgccac gaccggcgat gacaacgggg
      961 ccgccggcac ggcgtcgcta tttgtcggtg cagagttgac gcctgcggcc ttgcgcggcc
     1021 gtcgcgcggc cgcatcgtct cttgagcgca tcgaagacct cacactgcca aagggatggg
     1081 acagctcgac gccgacgatt ccacttgggg tcaacccaga gggggacgcc ggtctgagtg
     1141 cctacgtgcg ccgaagtgtc gtcgaggcgt actcgtgctc tcggaaagtg gccgagttga
     1201 ccggtcggag tgcggcggcg accctgggtt atcctgacac cggcctggcc agcaagctcc
     1261 ggacgattgc gcagctcttg aaagtcgatc tgggcgcgcg agtctattac acgactcaac
     1321 cgggctacga tacccacgcc ggccagtcca acacccatta tggtcttttg cgggagctct
     1381 caggcgcaat caaggcattc ttggacgacc tgactgcggc aaacctggct gagcgcgtaa
     1441 cggttctttg ctttagcgag ttcggccgcc gagtagccga gaatagctcg gcgggcaccg
     1501 accacgggac ggccggcttg gtgctgctcg ccggcccgcg tgtgcgtccg ggtgtgcacg
     1561 gcggagtacc cagtctcacg gacctggtcg acggcgaccc gaagatctcg accgactttc
     1621 gaacagtcta cgcggcccta cttgaagact ggcttggctt gccgagccaa accgcactgg
     1681 gaacaagatt tgaaccgatg ccactctttt ggctgacccg tgcgtgattg acggaacagc
     1741 aactcgattc aagagccttg gcctttcttc tctccagtca ccgatctctg acttgcgtcg
     1801 tgtgtagggc tggccgccgg tgtaatcttg agctggtgat cgagtacggg taccgaaata
     1861 ccaaaagaaa gtttccttaa tccagttcat gttacatttt tatactactg gatgtgttct
     1921 gaccgagtgg acaggccatg tcggaagtcc ctatcttggc accgcgttcg tcactatcga
     1981 tgatcacttc gtcggatcag ctggtcgaag agggcttcca gttcccgtgc gagcacggat
     2041 tgcgcctccg gactttccag ggcgagcgcg tcgaggcgaa acacctgcct catgagctgg
     2101 attcccgcga tgatggacag caacacagcg gcgcgctctc cggcgttcgc gccgggcaac
     2161 atggccgcca gaggcgcctg gaagttccgc tcgatggcgt cgtgaatgat ggtcacggcc
     2221 tgagggttcg agctcgaacg tagcaggagc aggaagccgt ctagccctcg cgccggcgtt
     2281 gccgccaggg cggcggctgc ctcttggccg agcgtgccgg catggcccgt taggatgctc
     2341 tttgtgcttt gcatattatc taccacctcg gcaaagagct gctccttcga gccgaagtat
     2401 cggttgacca tcatcgcggt cacgccggcg ccctgcgcga tctcgcggac gccaaccccg
     2461 tcatatcctg accgggtgaa ggcgatccgg gccgattgca ggatcgcctc gcgcgtcgcc
     2521 gcgcctttcc tgccctgctc gggcgcggcg gcgccggccc cttttgtgcg cttgccacgt
     2581 gtcatgtcta ccactgtaga ctttttcgat cggctcgtct atactgcttc tgtcggccgg
     2641 gc
//
LOCUS       DATAQL010000358         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4463503, whole genome shotgun sequence.
ACCESSION   DATAQL010000358 DATAQL010000000
VERSION     DATAQL010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4463503"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..814)
                     /gene="atpC"
                     /locus_tag="VHS97_06190"
     CDS             complement(407..814)
                     /gene="atpC"
                     /locus_tag="VHS97_06190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX3447823.1"
                     /translation="MPGDKKLQCVVVTPEKTLFDERVDFVALPLYDGEAGILPGRMPL
                     IGRLGFGELRTKTGDVVKRYFVDGGFVQVRADVVTVLTNRAVPAQELDAAAAAKELEA
                     AGSQTVISDSEFVEQEKAIARARGKIRVARHKG"
     gene            complement(925..2361)
                     /gene="atpD"
                     /locus_tag="VHS97_06195"
     CDS             complement(925..2361)
                     /gene="atpD"
                     /locus_tag="VHS97_06195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX3447824.1"
                     /translation="MATATTTGQISQVIGSTFDAEFDEGHLPNIYNALRIDDDVKGVK
                     IHLTGEVQQHLGGNRVRCVALGSTDGLVRGMPVVDTGGPVSVPVGKGTLGRVFNLLGD
                     PIDGRGPVHADEQWPIHRDPPAFDDLSPKTEQFETGIKVIDLLTPFVRGGKIGLFGGA
                     GLGKTVILTELIARIATVHSGFSVFAGVGERTREGNDLWLEMQESKIGSTGKSVIDST
                     VMCFGQMNEPPGARLRVALSALTMAEWFRDSTGADTLLFVDNIFRFSQAGSEVSALLG
                     RMPSNVGYQPTLATEMGALQERITSTKKGAITSVQAVYVPADDLTDPAPATTFGFLDA
                     FIVLSRSISEKGIYPAVDPLGSTSRILDPQYVGQEHYDVARRVQQSLQRYKELRDIIA
                     ILGVDELSEEDKLIVHRARRIERFMSQPFFVAEVFTGKAGQYTKLPDTIRSFKEICEG
                     KWDHLPEGAFMYVGAIEQAEEQAKKMSA"
     gene            complement(2392..3318)
                     /gene="atpG"
                     /locus_tag="VHS97_06200"
     CDS             complement(2392..3318)
                     /gene="atpG"
                     /locus_tag="VHS97_06200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX3447825.1"
                     /translation="MAKARAIIKRRKAVDNIRKITRTMELIATNRFRKALNRATEAEA
                     YTRKIAELVADLGETSGDIKHPLLTRHDPVKRALLLVITSNRGLAGGYNGNILRAAIL
                     RHHDNQTDGIETSFEVAGKRGIGFFRFRKLVPDATYTQFEDRPQFHEVEILAEKYIKL
                     YVQGTIDRLDVAYTQFISSSRQVATVQTLLPITTADLGKTTPSVRQASPAAKEAPAPA
                     KKVEPTSYEFLPDAKSILEEIVPVSFKVQLFKCFLDAAVSEQIARMVAMGGATKNADD
                     LVKSLTRQYNRARQNQITRELADIVGAAGALE"
     gene            complement(3378..>3938)
                     /locus_tag="VHS97_06205"
     CDS             complement(3378..>3938)
                     /locus_tag="VHS97_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX3447826.1"
                     /translation="LPIIETLEGEVSAYIPTNVISITDGQIYLQPDLFFAGVRPAIDV
                     GISVSRVGGKAQIPAMKKVAGGLRLDLAAFRELEAFAQLGTELDKATQKQLDRGYRMV
                     ELLKQPQYKPMNAIDQVMSIFAGTQGHLDDVPRVDVPRWETSFLNFVKDRKPEVRAAL
                     EREQKMTPKIEADLSSAIAEFKAQFK"
BASE COUNT          724 a         1199 c         1217 g          798 t
ORIGIN      
        1 ggcttcattt cctccctccg gacgatcagg ccatccagta attcgacccg ctcgtcgtca
       61 agtgaatccg cgatccgctc gaactcgtct accgacatgc ggtaaacgac cggagatgcc
      121 aagggggcag gcgacgacat cggttgtgtt ggagtaaccg tagacattct cgcgaatccc
      181 tgttccagga cgtccgatgc caagtggcct gcaaagaagc ccgtccgtta ggatactgca
      241 aacgcgcggg aaacctccac ggcaaaaact cctccgcgca ggccgaacga cgctccgcga
      301 gacgtgtgct ggcaagaagg tcaagccgat gggcctgggt tgacctcgcg aggatcgaat
      361 tcggcgacgt cgggattgat ctaacgattc gcttcggaac tcacaatcag cccttatgcc
      421 tggccacgcg aatcttcccg cgcgcgcggg cgatcgcctt ttcctgctcg acgaattcgc
      481 tgtcgcttat gacggtctgc gagcccgcgg cttcgagctc cttggctgcg gccgccgcgt
      541 cgagctcctg ggccgggacg gcccgattgg tcaagaccgt gacgacgtcg gctcgcacct
      601 gcacaaagcc gccgtcgaca aagtaacgtt tcacaacgtc accggtcttg gtccgcaatt
      661 cgccaaagcc cagccggccg atcaacggca tgcggccggg caagatgccg gcctcgccgt
      721 catagagcgg cagcgcgacg aaatcgaccc gttcatcgaa cagggttttc tccggcgtga
      781 cgaccacaca ctgaagcttt ttatcgccgg gcatcgatgt cctctcgggg gaacaccccc
      841 caccctggcc cttcgccaca aggggggagg ggattataaa tacctatgag ccgctgatct
      901 actgatactt caatggcatc gcaatcaagc gctcatcttc ttggcctgct cttcggcctg
      961 ctcgatggca ccgacgtaca tgaaggctcc ctcggggaga tgatcccact tgccttcgca
     1021 gatttccttg aagctgcgga ttgtgtcggg aagcttcgta tactgccccg ccttgccggt
     1081 gaacacctcg gcgacgaaga acggctgcga catgaaccgc tcgatccgcc gggcacgatg
     1141 aacgatcagc ttgtcttctt ccgatagctc gtcgacaccc aggatcgcga tgatatcccg
     1201 cagctccttg tagcgctgca ggctctgctg aacgcggcgg gcgacgtcgt agtgctcctg
     1261 acccacatat tgcgggtcga ggatgcgcga cgtcgagcca agcgggtcga ccgccgggta
     1321 gattcctttc tcggagatcg accgcgacag aacgatgaac gcgtccagga agccgaaggt
     1381 cgtcgcgggt gcgggatccg tgaggtcgtc ggccgggaca tacacggcct gcaccgacgt
     1441 gatggccccc ttcttggtcg aggtgatgcg ttcctggagc gcgcccatct cggtggcaag
     1501 ggtcggttga tagccgacgt tcgagggcat acgtcccagg agggccgaga cctccgagcc
     1561 ggcctggctg aaccggaaga tgttatcgac aaagagcagc gtgtcggcgc cggtcgaatc
     1621 ccggaaccac tctgccatcg tcagcgccga cagggcgacc cgcagccggg cgccgggagg
     1681 ctcgttcatc tggccgaagc acatgaccgt gctgtcgata accgacttgc cggtgctgcc
     1741 gatcttggac tcttgcattt cgagccagag gtcgttcccc tcgcgcgttc gttcgccgac
     1801 acccgcgaag accgagaatc cgctgtgcac agtggcgata cgcgcgatca gctcggtcaa
     1861 gatgaccgtc ttgcccagcc cggcgccgcc gaagaggccg atcttgccgc cgcggacgaa
     1921 cggggtcagt aagtcgatga ccttgatgcc ggtctcgaat tgctcggtct tgggcgagag
     1981 atcatcgaag gcggggggat cgcggtgaat gggccactgc tcgtcggcat gcaccggtcc
     2041 gcgtccgtcg atcggatcgc ccagcaggtt gaaaacgcgg ccgagcgtcc ccttgccgac
     2101 gggcacactc acgggaccgc ccgtatcgac cacaggcatg ccacgtacca ggccgtcggt
     2161 cgaccccagc gctacacacc gcacgcgatt gccgccaagg tgctgctgca cctctccggt
     2221 caagtggatc ttgactccct tgacgtcgtc gtcgattcgg agggcgttgt agatgttggg
     2281 cagatgacct tcgtcaaact cggcgtcgaa cgtcgagccg atcacctgcg aaatttgtcc
     2341 cgtcgtcgtc gcagtggcca tgatgaagaa ctccttgatt ctatgcgttt actattccag
     2401 cgcgccggcc gcaccgacga tgtcggccag ctcgcgcgtg atctggttct ggcgggcacg
     2461 gttatattgc cgcgtaagtg atttgacgag atcgtcggca ttcttggtgg ccccccccat
     2521 ggcgaccatc cgagcgatct gctcgctgac agccgcatcg aggaagcatt tgaacagctg
     2581 gaccttgaat gacactggca caatttcttc gagaatgctt tttgcgtcgg gcagaaactc
     2641 gtacgaggtc ggctcaactt tcttggccgg cgcgggcgcc tctttggccg caggactggc
     2701 ttgccggacc gaaggggtcg ttttgccaag atcggcggtc gtgatcggta acagtgtctg
     2761 taccgtcgcg acctggcgcg acgatgagat gaactgggtg tacgccacgt ccaggcggtc
     2821 gatcgtcccc tggacgtaga gcttgatgta tttctccgcg aggatctcga cctcgtgaaa
     2881 ctgaggacgg tcctcgaatt gggtgtaggt cgcgtcggga acgagcttgc ggaagcggaa
     2941 aaagccgatt ccgcgcttgc ccgcgacctc gaacgacgtc tcgattccgt cggtctggtt
     3001 gtcgtggtgg cggaggatcg ccgctctcaa gatgttgccg ttgtaaccgc ccgccaatcc
     3061 acgattgctc gtgatcacga gcaagagcgc tcgcttgacc ggatcgtgcc tcgtcaagag
     3121 cgggtgcttg atgtcgcccg acgtctcccc gaggtcggca accagctcgg cgattttgcg
     3181 cgtataagcc tcggcctccg tggcccggtt gagcgccttg cgaaaccggt tggtcgcaat
     3241 cagctccatc gtccgggtga tcttgcggat gttatcgacc gccttgcggc gcttgatgat
     3301 cgcgcgggcc ttggccattg agttttacca gtcgctagtt gccagatctg ggtttcaatt
     3361 gggcgagaaa cgattactta tttgaactga gccttgaact ccgcaatggc gctcgacagg
     3421 tcggcctcga tcttgggagt catcttctgt tcgcgttcca atgcggcgcg cacctcgggc
     3481 ttgcggtcct tcacaaagtt caagaaagag gtttcccagc gcgggacgtc aacgcgggga
     3541 acgtcatcca agtgtccctg agttccggcg aagatactca tgacctggtc gatggcattc
     3601 atgggcttgt actggggctg cttgagcaac tcgaccatac gatagccgcg gtcgagctgt
     3661 ttctgagtgg ccttgtccag ctcggtgccg agctgggcga aggcttcgag ctcgcggaag
     3721 gccgccaggt cgaggcgtag accgccggcg acctttttca tggccgggat ctgggccttg
     3781 ccgccgacgc gcgagacact aatgccgacg tcgatcgcgg gccgcacgcc ggcgaagaag
     3841 aggtcgggct gaaggtagat ctggccgtcg gtgatcgaaa tcacgttggt ggggatgtag
     3901 gccgaaacct ctccctccag ggtctcgatg atcggcaa
//
LOCUS       DATAQL010000359         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1256900, whole genome shotgun sequence.
ACCESSION   DATAQL010000359 DATAQL010000000
VERSION     DATAQL010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256900"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHS97_06210"
     CDS             <1..323
                     /locus_tag="VHS97_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447827.1"
                     /translation="PAVSIERDYGYVRHHPLAEAYFRRNPPPHNRPTWDLTSVLYAVQ
                     PDRGYFDVSASGTVTVEPDGFARFDENPQGKHRYLFLRPEQRPRVLEALVQLSSQPPT
                     GGRE"
     gene            335..2197
                     /locus_tag="VHS97_06215"
     CDS             335..2197
                     /locus_tag="VHS97_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3447828.1"
                     /translation="MRSRQLVHLVAVLLAIMVAHANAPGQTVATVAPPSWWVEAHEQS
                     LMLLIEGTNLDGALVRVARGPIRVSRVEAGREGRALFVELIVPAGARSGRCQIEIEVQ
                     GHTFAREWELVDRPARRPEPFGPDDVIYLVMVDRFANGNRENDDPPSGDRLFDRRDSH
                     AYHGGDFAGLRRRLPYLADLGITAIWLTPLYRPSSTWFAGNLGGTPRKMADFHGYCPV
                     DFYDTNPRFGSLDDYRAMVDEAHRLGLKVIQDQILGYTGPKHPWVAHPPTVDWFHGPL
                     DRPPMCNFRFDALTNPHARETDRVGLTDGWFFGILPDLNVRDSRVSRYAIQQSLWWVT
                     MFGADGVRLDTYPMVDRSFWRDWSRRLKATHPMIRTVGEAWVVDASDLSFFQGGRPGW
                     DGVDPGVDTVFDFPLNQAAVAVFSGRAPASALAQALRRDGLYPRSDLLVTFLDNHDTA
                     RFAGLPDMTDERLQLAVAFLLTTRGIPQITWGDEIGMSGHMDDRRDFPGGFPDDPRDA
                     FTGAGRTPREQAIYTTYCELLRLRKGSPALRRGTLTDLIADGSIFAYLRQNGTDRVIA
                     ALNGGKAKAEVPLPAEIDGSLERLYGKAEWFKATDCVRLTVPGESAAIFRLAGH"
     gene            complement(2259..>3230)
                     /locus_tag="VHS97_06220"
     CDS             complement(2259..>3230)
                     /locus_tag="VHS97_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447829.1"
                     /translation="EPTTGLHFDDVRKLLDVIHRLADLGNTVVVIEHNLEVIKTADWV
                     IDLGPEAGIGGGDLVAEGTPEHIVKNRHSHTGRFLKPVLAAGPLAERPRFILQSKSIR
                     VKTDERGGKSGSRKGDHIKGGKPTDAKISRLQSEISAEAQAPWEIDGRKWHTRDRLAK
                     NGRPVRWDGRILERIVDQIEALGNSHASTKTDSGFAPTDWSQRNLVKIHGSDPAKISF
                     PFLHATTSSEWVILLRFFVPQNTFRERSLESELELIPFHESETPVLSDQPRLKITEIG
                     PFQEITIVGHAAEDFETPAFDSFLRQAVGAFLAIGKPSKLMRASELG"
BASE COUNT          572 a         1005 c          988 g          665 t
ORIGIN      
        1 acccggcggt gagcatcgag cgcgattatg gctacgtgcg ccatcatcct ctcgccgagg
       61 cttacttccg ccgcaatccg ccgccgcaca accggccaac ctgggacttg acgagtgtgc
      121 tctacgcggt tcaacccgac cgcggttatt tcgacgtctc cgcaagcgga acggttaccg
      181 tcgagcctga tggcttcgcg cggttcgacg agaacccgca aggcaagcat cgctacctgt
      241 tcttacgacc cgagcaaagg cctcgcgtac tcgaggctct cgtgcagctt tccagccaac
      301 cgccgaccgg cgggcgtgaa taggtgtgga accgatgaga tctcgccagc tcgtgcatct
      361 ggtcgcggtc ttgctcgcga tcatggtcgc ccacgcgaac gctccaggtc agaccgtggc
      421 gaccgtcgcg ccgccgtcgt ggtgggtgga agcgcatgaa caatcgctca tgctcttgat
      481 cgaggggact aacctggatg gcgctctcgt ccgcgtcgcg cgggggccaa tccgtgttag
      541 ccgtgtcgag gcggggcgcg aggggcgcgc actgtttgtc gagttgatcg tccctgccgg
      601 agcacgctcg gggcgctgtc agatcgagat cgaagtgcag ggccatacct tcgcgcgcga
      661 gtgggaattg gtcgaccggc cggcccggcg gccggagccg tttgggccgg acgatgtgat
      721 ctacctggtc atggtcgacc gttttgcgaa cgggaaccgg gagaacgacg acccgccgag
      781 tggcgatcgc ctgttcgacc gccgcgattc ccacgcatac catggcggcg attttgctgg
      841 cctccgccgt aggctcccat acctggccga cctgggcatc accgcgatct ggctcacccc
      901 gttgtaccgc ccttcgtcga cgtggtttgc gggtaacctc ggaggaacgc cgcgcaagat
      961 ggccgacttt cacggatact gcccggtcga tttctacgac accaacccac gcttcgggtc
     1021 gttggacgac taccgcgcga tggtcgacga agctcatagg ctcggcctca aggtaatcca
     1081 ggaccagatc ctcgggtaca cagggccgaa acacccctgg gtcgctcatc cgccgactgt
     1141 cgactggttc catggtccgc tcgaccgccc gccgatgtgc aacttccgct tcgacgcctt
     1201 gacaaaccct cacgctcgcg agaccgatcg cgtcggtctg accgacggct ggtttttcgg
     1261 aatcctgccc gacctcaacg tacgtgactc gcgtgtgagc cgttatgcca ttcagcagtc
     1321 gctctggtgg gtcacgatgt tcggggccga cggagtccgg ctggacacgt acccgatggt
     1381 tgaccgtagt ttttggcgcg actggagcag gcgtctcaag gcaacccatc ccatgatccg
     1441 aacggtaggc gaagcatggg tcgtcgacgc gtccgacttg tccttcttcc aggggggacg
     1501 accgggctgg gatggagtcg accctggtgt ggatactgtc tttgacttcc cgctcaacca
     1561 ggcagccgtt gcggttttct cgggacgcgc tcctgcgtcg gcactggcgc aggcgcttcg
     1621 gcgtgacggg ctctacccgc gctcggatct gctggtgacg ttcctggaca accacgacac
     1681 agcgcggttc gctggcctac ccgacatgac cgacgaacgg ctgcagctag cggtcgcatt
     1741 cttgctgacg acgcggggca tcccacaaat cacctggggc gatgagattg gcatgtccgg
     1801 tcacatggac gaccgccgcg attttccagg cggtttcccc gacgatccgc gcgatgcgtt
     1861 cactggcgcc ggacgaacac cccgggaaca agcaatttac accacctact gcgaactttt
     1921 gcggctcaga aaaggctccc ccgctctgcg acgaggaact ctcactgact tgattgccga
     1981 tgggtcgatc tttgcctatc tgcgccaaaa tgggaccgac cgagtgatcg ctgccttgaa
     2041 cggcgggaag gccaaggccg aagtaccgct cccagcggag atcgatggat cgctcgagcg
     2101 cctgtacggc aaggccgagt ggttcaaagc gaccgattgt gtccgtctca ccgtgcctgg
     2161 tgagtctgcc gcaatctttc gtctcgccgg acattaaccc agttttcggc accgcatgca
     2221 cggtcttcca ccggaaaccc tgacatgaca ggcggagctc agccgagttc gctcgctctc
     2281 atgagcttgc tgggtttccc gatcgcaagg aaagcgccga cagcctgtcg caagaacgag
     2341 tcgaatgcgg gcgtttcgaa gtcttcggcg gcatgaccga cgatcgtgat ttcctgaaac
     2401 gggccgatct cggtgatttt caatcgcggc tggtcgctaa gcacaggcgt ttcactctcg
     2461 tgaaatggaa tcagttcgag ttcactttcg agcgatcgtt cgcggaacgt gttttgggga
     2521 acaaaaaacc gcagtagaat gacccactcg gatgatgtcg ttgcgtgcaa gaaagggaag
     2581 ctgatcttgg ccggatccga cccgtgaatc ttcacgagat tgcgctgcga ccaatctgtg
     2641 ggcgcaaagc ctgagtcggt ttttgtactg gcgtgggaat tgcccagggc ctcgatctgg
     2701 tcgacgatcc gttcgaggat gcgtccgtcc caccggaccg ggcggccgtt cttggcgaga
     2761 cggtcacggg tgtgccattt gcgtccgtcg atttcccagg gagcctgggc ctcagcagag
     2821 atttcggact gcagacgaga tatttttgca tctgtgggtt ttccgccttt gatgtgatct
     2881 ccttttcttg agcccgactt tccaccccgc tcgtccgttt tcactctgat ggatttactc
     2941 tggagaataa accgcgggcg ctcggctagc ggcccggccg cgagcacggg cttcaggaac
     3001 cggccggtgt ggctgtgccg atttttgaca atgtgctcag gagtgccctc ggcgaccagg
     3061 tcccctccgc cgatacccgc ctcgggaccg aggtcgatga cccaatcggc cgtcttgatc
     3121 acctcgagat tgtgctcgat gaccacgacc gtgttaccca gatcggccag acgatggatc
     3181 acgtccagca gcttgcggac atcgtcgaaa tgcagcccgg tcgtaggctc
//
LOCUS       DATAQL010000360         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_340631, whole genome shotgun sequence.
ACCESSION   DATAQL010000360 DATAQL010000000
VERSION     DATAQL010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340631"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VHS97_06225"
     CDS             complement(<1..773)
                     /locus_tag="VHS97_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3447830.1"
                     /translation="MTPRRALVILIVSSALIRLIAAFNLGLGNDEAYHFLYAAHPALS
                     YYDHPPMMAWVEMAGLALSGNHATSWALRLGFIVLFGCSTWVLAKLTSRSYGEWPGFL
                     AALALNVTGYYGLAAATLALPDGPLLFFWLLTIDRLTVALDEPDPRRLRPWVWVGLAW
                     GGALLSKYHAVLIPLAVCLYALLHRPMRRLIFQPGPLLALAIGLLLFSPVIAWNASHG
                     WASFIFQGSRAVGSSLPRPDYLLVAILAQAGYLFPWIWVP"
     gene            927..1778
                     /locus_tag="VHS97_06230"
     CDS             927..1778
                     /locus_tag="VHS97_06230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX3447831.1"
                     /translation="MTSPDSSHSDMIAQADNDARNIVDSAGSSLADRAEIDKSDQRVR
                     RMFASIAHRYDLLNHLLSLNIDRSWRSYTTRIVAPVAGVPILDCCTGTADLALAYDAA
                     GRGGSPIVATDFCREMLELGHAKVQKAGAAARITLVEGDTQRLPVPSDTFGVVSVAFG
                     LRNVRDTIRGVDEMIRVARPGGKIAILEFSRPRGRFLGRLYLGFFRHLLPRVGQALAP
                     NQDGAYSYLPQSVLEFPDGQAMLDLLAARGLTELEQYPLTGGIATLYVGTKPLSAAGS
                     ENQESGT"
     gene            1841..2716
                     /locus_tag="VHS97_06235"
     CDS             1841..2716
                     /locus_tag="VHS97_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3447832.1"
                     /translation="MPGDIGAIKSAPHPVVLLTGASAGIGAALARELVRRKKAKALVL
                     TARRGDRLDHLATELLALQPDLVVLTIAADLAEPETPERLLDATIARFGELDVLINNA
                     GLGLPTLFAGSIPEHLARQLTVNFTAPLMLTRICLPSLIERKGMIINIGSAITCVANS
                     ALGAYGATKAGLAYWNDALRRELHSKGVTVCLVEPGPIKTEFMDALSSLVPAGKETHP
                     ILDNAAPWMTADVDLVARGVVGLIDHPRRRLSVLRRFVWPFRILGAIARICPPLGDRM
                     VRLLQRESQEAREAT"
BASE COUNT          624 a          967 c          932 g          562 t
ORIGIN      
        1 ggtacccaga tccatggaaa caagtagccc gcctgagcca ggatcgccac caacaagtaa
       61 tcgggccgag gcaacgagct ccccaccgcg cggcttccct gaaagataaa cgacgcccag
      121 ccatggctgg cattccaggc gatgaccgga ctgaataaga gcaagccgat cgcgagggca
      181 agaagaggtc caggctggaa gatcaagcgt ctcatcgggc gatggagcag agcgtaaagg
      241 caaaccgcca aagggattaa caccgcgtga tacttgctca agagcgctcc gccccaggcg
      301 agcccgaccc agacccaggg cctgagcctg cgtgggtcgg gctcgtcaag tgccaccgtc
      361 aatcgatcga tcgtcagtag ccagaagaag agtaacggac cgtccggcaa cgccaacgta
      421 gcggccgcga ggccatagta gcccgtgacg ttgagcgcca atgccgcgag aaaccccggc
      481 cattcgccgt aagatcgact cgttagtttc gccagcaccc aggtcgaaca cccaaaaagc
      541 acgatgaaac ccaacctcag tgcccaggag gtggcatggt tgcccgataa cgccaggcct
      601 gccatctcaa cccacgccat catcggggga tgatcgtagt aactcagggc gggatgggcc
      661 gcgtacagaa agtggtatgc ctcgtcgttt cctaacccca ggttgaacgc cgcaatcagg
      721 cgaattaacg cggaagagac gatcaagatc acgagcgcgc gtctgggggt cattatgcgt
      781 ccttgctttg acctgagcgc aagaaaccca agcgaggggc cgatttaccc tgatcggaaa
      841 atccaagccg tgcgccttgt cgacttgcgc ctgggtggcc cacaataatc acgacaaacc
      901 cgttgagcgt caacgccgac cgaaaaatga cgtcacccga ctcgagtcat agcgacatga
      961 tcgcccaggc tgataacgac gcgcgaaaca tagttgattc agcagggtcg tcgctggctg
     1021 accgggcgga gatcgacaag tccgatcagc gcgtgaggcg gatgtttgcc tcgatcgcac
     1081 atcggtatga tttgctcaac cacttgctca gcctgaacat cgatcgttcc tggcgaagct
     1141 acacaacacg gatcgtggct cccgttgcag gtgtaccaat tctcgactgc tgtacgggta
     1201 ctgccgacct ggccttggcc tatgacgctg ccggccgcgg tggttcgccg atcgtcgcga
     1261 ccgatttctg tcgcgagatg ctcgagttgg gccatgccaa ggtccagaag gcgggcgcag
     1321 cggcacgcat cacgcttgtg gaaggagata cccagcgctt gcctgtgccc agcgacacct
     1381 ttggtgtggt tagcgtggct ttcggcctgc gcaacgtgcg cgacacaatc cgcggcgtcg
     1441 acgagatgat ccgcgtcgca cgaccgggcg gcaagattgc catcctcgag ttttcccgtc
     1501 ccaggggacg gtttttgggc cggctctacc ttggcttctt ccgccatttg cttccacgcg
     1561 tcggtcaggc gctggcgccc aatcaggacg gcgcttacag ctatttgccc caaagcgttc
     1621 tggaattccc ggacggccag gcgatgctcg atctcctggc cgcacgcggg ctgactgagc
     1681 tcgagcaata tccgctgaca ggcggcatcg ccaccctcta cgttggcacc aagccactct
     1741 ctgccgccgg gtcagagaac caggagtcag gcacatgagt cttgctgtgc cgtcgtcctc
     1801 ggccagaccc agcccgaagc aatcgaggaa ccgtgaaacg atgcccggcg acattggcgc
     1861 catcaagtcc gcgccgcatc ccgtggtcct cttgaccggg gcgtccgccg gcattggcgc
     1921 ggccctggca cgcgagttgg tccgacgaaa gaaggcaaaa gcactggtcc tgaccgcccg
     1981 ccggggtgac cggctcgatc atctcgccac cgagctcttg gcactacagc ccgatctggt
     2041 ggtcctgacc atcgcggccg atctggccga gccggagaca cccgagcggc tgctggacgc
     2101 aacgatcgct cggtttggcg aactcgacgt gctgatcaat aacgccggtc taggtttgcc
     2161 cactcttttc gccggctcta tccccgagca cctggctcgc cagctcacgg tcaattttac
     2221 cgccccgctt atgctgactc ggatatgctt gccttcattg atcgagcgca aggggatgat
     2281 tatcaatatc ggctcggcga tcacttgcgt ggccaactcg gccctcggcg cgtacggtgc
     2341 gaccaaggcg ggcctggcct actggaacga cgccttgcgc cgcgagttgc actcgaaggg
     2401 agtcacggtt tgcttggtcg agcctgggcc gatcaaaacg gaattcatgg acgccctcag
     2461 ttcactcgtc ccggccggca aagagaccca tcccatattg gacaacgccg caccatggat
     2521 gacggccgat gtcgacttgg ttgctcgtgg cgtcgtgggc ctgatcgatc atccccgtcg
     2581 gcggctgtcg gtcttgcgga ggtttgtctg gccgttccgg attttgggcg cgatcgcgcg
     2641 gatctgtccg ccgctcggag accgaatggt caggctgttg caaagggaga gccaagaagc
     2701 acgtgaagcc acctgaacct cgacgactca gacgcaggca tggatcaaac gtttcgtcag
     2761 cgacgggtgc ggtgtcgatt ctttattgag tgcattgcca tccaggaggt gccgggccag
     2821 tgcgccgatc tcgccgagag cggcagaatg cagcgtgcac tgccgcagtg tgcagccgcg
     2881 attttcggca tcgctgaacg cagacaggtc gttcttgccc caggttgatc gccagagagg
     2941 tcgccccaca cccgcccgtg acaccggcca cggcgaagac ccttgaccgg gcgctcgctg
     3001 aggtcgcaga cgtttcggcg attcgcttga gcgcagtggt gaaatcctgg ggattcagcg
     3061 gcaggcccac gatctgcgac acccc
//
LOCUS       DATAQL010000361         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3242813, whole genome shotgun sequence.
ACCESSION   DATAQL010000361 DATAQL010000000
VERSION     DATAQL010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242813"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1680)
                     /locus_tag="VHS97_06240"
     CDS             complement(217..1680)
                     /locus_tag="VHS97_06240"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX3447833.1"
                     /translation="MLKRILPAVAISVSFSAIAAAGGLPTTGSIDTPIGKLEISNGYP
                     TDATTVKLFDAMDFQRAVQAYLWALPMVGSAQWQNEQHEKFGAGDLDYVDYLDFKDKL
                     GLLTANATTPYSISFVNLAKTGPLVVEIPAGAIAGGILDVWQRPITDTGALGPEKSRG
                     GKFLILGPNDPDLKPDGYYVFRSPTNNIWSGQRGLDPDKAKAAALLGQIRNYPYAERD
                     NPKNVSKHIRPDGRKWAGQQPGGLHYWEVVARVINEEPPLERDRITLATLVPLGIEKG
                     KPFNPDARQKAILEQASNVGELMARANGYAKRFPGSTIWPGKHWEYSLFLKETDQEVP
                     THTQLDERASWFYEAVGVTVGMMGRTVGAGQVYLEASKDAAGNWLEGDKTYTLHVPKN
                     APVAQFWSFTVYDNETRCFVDTGTYPDRSSRDDIVVNGDGSTDLYFGPTPPPGKPEHN
                     WIKTIPNRGWFTYFRLYGPTQPYFDKTWQLPDIAEVK"
     gene            complement(1737..>2203)
                     /locus_tag="VHS97_06245"
     CDS             complement(1737..>2203)
                     /locus_tag="VHS97_06245"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX3447834.1"
                     /translation="GAYRDLDARDWFFTNYYSISPGMVSKIPGKGAAYMIGFSDNKGK
                     PLSGGSNYKLNLPPNIPAANFWSVTLYEAENASGLANGQPFPSLGSRDKPAQNADGST
                     DIYVGPKAPSGKEANWLATVPGRGFFAILRLYGPTEAALNRTWKAGDFEEIK"
BASE COUNT          374 a          635 c          746 g          448 t
ORIGIN      
        1 agcggaaccg aaagttcgat tcacgtcccc tcactcagat atggagccgg gccgggtttt
       61 ctggcgattg attgatgcac gagatgttgc acccttgagt tctcgactat caggcgcagg
      121 gcaggtgtcg caccgccatg catcaaaggg tggttttcta cgttcggacc gtaggaccgg
      181 agtggatgga aagcggactc ctgccttgcg agcgtgtcac tttacttcgg cgatgtccgg
      241 caactgccac gtcttgtcga agtagggctg ggtcgggcca tagagccgga agtaggtgaa
      301 ccagcccctg ttcgggatcg tcttgatcca gttgtgttcg ggtttgccgg gcggcggggt
      361 cgggccgaaa tagaggtcgg tcgatccgtc gccattgacg acaatgtcgt cgcgcgagga
      421 gcggtcggga taggtgccag tgtcgacgaa gcaccgggtc tcgttgtcgt agaccgtgaa
      481 cgaccagaac tgcgcgaccg gtgcattttt cggcacgtga agcgtgtagg tcttgtcccc
      541 ctccagccag ttcccggccg cgtccttgga agcttcgaga tagacctgac cagcgccgac
      601 ggtgcgcccc atcatgccga ccgtcacgcc gacggcctcg tagaaccatg aggcgcgctc
      661 gtcgagttgg gtgtgggtcg gaacttcctg gtccgtctcc ttgaggaaca gcgaatattc
      721 ccaatgcttg ccgggccaga tggttgagcc ggggaagcgc ttggcgtagc cgttggcgcg
      781 cgccatcagt tcgccaacgt tcgaggcctg ttcgagaatg gccttctgcc gcgcgtcggg
      841 attgaatggc ttgcccttct cgatgccgag cgggacgagc gtcgccagcg tgatgcggtc
      901 gcgctccagc ggcggctcct cgttgatgac gcgcgcgacg acctcccagt agtgcaatcc
      961 acccggctgc tggcccgccc atttgcgtcc gtccggccgg atgtgcttcg aaacgttctt
     1021 cgggttgtcg cgttcggcgt aaggatagtt gcggatctgc cccaggagcg ccgcggcctt
     1081 cgccttgtcg ggatcgaggc cgcgctggcc ggaccagatg ttgttggtcg gcgagcggaa
     1141 gacataataa ccgtcgggct tcaggtcggg atcgttgggg ccgaggatga ggaacttgcc
     1201 gccccgcgac ttctccgggc caagcgcgcc ggtgtcggtg atcggacgct gccagacgtc
     1261 gagaatgccg ccagcgatcg cgccggccgg aatttcgacc accagcggac cggtcttcgc
     1321 gagattgacg aaggagatcg aatagggcgt ggtcgcgttg gcggtcagca gtcctagctt
     1381 gtccttgaag tcgagatagt cgacgtaatc gaggtcgccg gcgccgaact tctcatgctg
     1441 ctcgttctgc cattgcgccg agccgaccat cggcagcgcc cagagatagg cctgaaccgc
     1501 gcgctgaaag tccatcgcat cgaagagctt cacggtcgtt gcgtcggtcg ggtagccgtt
     1561 cgaaatctcg agcttgccga tcggcgtgtc gatggagccc gtggtgggaa ggccgcccgc
     1621 ggccgcgatg gcggagaaag ataccgatat ggcaacggcc ggcaggattc tcttaagcac
     1681 gtcgatggct cctctggaga ctgcgttacg agagccgcac cggtgcaggt atgaactcat
     1741 ttgatctctt cgaagtcgcc cgccttccaa gtcctgttga gagcagcctc ggtcgggccg
     1801 tagaggcgca ggatcgcgaa aaagcccctc ccgggaacgg tcgcgagcca gttggcctct
     1861 ttgcccgacg gcgccttggg tccgacgtag atgtcggtgc taccgtcggc gttctgggcc
     1921 ggcttgtcgc gcgagccgag cgaaggaaag ggctggccgt tggcgaggcc ggaagcgttt
     1981 tcggcctcgt agagcgtcac cgaccagaag ttggcagccg ggatgttggg gggaaggttc
     2041 agtttgtagt tcgatccgcc ggacagcggc tttcctttgt tgtcgctgaa gccaatcatg
     2101 taggccgcgc ccttgcccgg aatcttcgat accataccgg ggctgatcga gtagtagttg
     2161 gtgaagaacc agtcccgcgc gtcgaggtcg cgataggcgc ccg
//
LOCUS       DATAQL010000362         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5379565, whole genome shotgun sequence.
ACCESSION   DATAQL010000362 DATAQL010000000
VERSION     DATAQL010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5379565"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VHS97_06250"
     CDS             complement(<1..435)
                     /locus_tag="VHS97_06250"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEX3447835.1"
                     /translation="MNSDFRDLLKLFNAHRVRYLVVGGYAVMKYTEPRYTKDLDVWVE
                     ATPKNAKAVFKALRKFEAPLANLHELDFATEGFCYQMGRPPARVDILMSIQGVRFADA
                     WSSRVASDFDGVTGHVISRKDLIANKRALGRPQDLVDVNSLVE"
     gene            complement(748..1374)
                     /locus_tag="VHS97_06255"
     CDS             complement(748..1374)
                     /locus_tag="VHS97_06255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX3447836.1"
                     /translation="MDKPIDPRRRRRPDAVTAIMMAVTATALLASIWLKTKGTPENEP
                     PRVGALAPALRLLDMETSEPLALVGQRGKVVWVFFWSATSPSPSSSLAAIERASVPLR
                     ARRRFAVVAAAVETDQSARVRAAVAESRSKLPVYLASAETLARYGVGVGAPPLHILID
                     ADGRIASIARESGPQTVERIADDAKRLLEELDPLGDTRFASAALHKFY"
BASE COUNT          300 a          476 c          433 g          311 t
ORIGIN      
        1 ctcgacaagg ctatttacat cgaccaagtc ttgcggtcgt ccgagtgcac gtttgttagc
       61 aatcaaatcc tttcgtgata taacgtgtcc agtaacgccg tcgaagtcgc tcgccactcg
      121 gctagaccag gcatcggcga agcgaacgcc ctggattgac atcaagatgt ccacacgggc
      181 cggagggcgt cccatttgat agcagaatcc ctccgtggca aagtccaact cgtgcagatt
      241 cgccagcggg gcttcgaatt tccgaagcgc cttgaatacc gccttggcat tcttcggagt
      301 agcctcgacc cagacatcca agtcctttgt atagcgcggc tcggtgtact tcatcaccgc
      361 atagccgccg accacgaggt agcggaccct atgggcgttg aaaagtttca acagatctct
      421 gaagtcggag ttcatccggg ctcatgcctc gatcgcggca gtacgattcg accagctccc
      481 aggccgcgcg gaagatcgcg gcgtccccct gtcgttgcca gaactcgatg tcaaacgaac
      541 gatccatgtc ttcgaggcga ccgaaacgca tcatcgcaaa gtcagatcgt tcggacgacg
      601 ccggcgcccg gctggcctcc gagccgtcag cttgcttccg tctcgctgat ggttctgggg
      661 acctgttcct catcgacgac atttcctctt cctccctcgt ataagacact tctcgattca
      721 gaaatggcca tgcacgcgaa ttgaatttta atagaatttg tgtagcgccg cactcgcaaa
      781 ccgcgtgtca cccagcgggt ccagttcctc gagcaggcgt ttggcgtcgt cggcaatccg
      841 ttcgaccgtt tgcgggcccg attctcgagc gatcgacgcg atccgtccgt cggcgtcgat
      901 caagatgtgc agaggagggg cgccgacgcc cacgccatac cgcgcgagtg tctcggcgct
      961 cgcaagataa accggcagct tgcttcggct ctcggcgacg gccgcgcgta ctcgagcgga
     1021 ctgatcagtc tccacagcag ccgccaccac ggcgaaccgg cgtcgtgccc ggagcggaac
     1081 tgaggctcgt tcgatcgccg ccaagctcga tgacggtgac ggagaggtgg ctgaccagaa
     1141 gaaaacccac acgaccttgc cgcgctgtcc gacaagcgcc agcggttccg acgtctccat
     1201 gtccaggagc cgcagcgcag gcgccaatgc gccaacgcgc ggtggctcgt tctccggcgt
     1261 ccccttggtc ttcaaccaaa tcgaagccag cagtgccgtg gctgtcaccg ccatcatgat
     1321 cgcggtgacc gcgtcaggcc gccggcggcg acgagggtcg atcggtttat ccatcaattt
     1381 gccttgtgtt agcgcccgct tgccttggga gagagagtaa cgatggtatc aatcggcaga
     1441 cgaatctcgg tcggtagttc caagcccagg ctccccttcg cttgctttag cgcgaggggt
     1501 gttgatttgc cgaataagta
//
LOCUS       DATAQL010000363         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5379680, whole genome shotgun sequence.
ACCESSION   DATAQL010000363 DATAQL010000000
VERSION     DATAQL010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5379680"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(458..1489)
                     /locus_tag="VHS97_06260"
     CDS             complement(458..1489)
                     /locus_tag="VHS97_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3447837.1"
                     /translation="MIIDCHGHYTTEPQKLHDFRKNQIAAIKDPGQKPSRATLNISDD
                     ELRGSVEGAQLKFQRERGTNVTIFSPRASGMAHHIGNEGVSLEWSQVSNELIYRLTTL
                     YPNNFIGVCQLPQSPGVNPKNCIGELERCVNEFGFVGCNLNPDPSGGHWDAPPISDRF
                     WYPLYEKIAELDVPAMIHVSASCNPAFHTTGAHYINGDTTAFMQLITSDLFKDFPTLK
                     FIVPHGGGAVPYHWGRYRGLAQDMKRPPLEEHLLNNVFFDTCVYHLPGIELLTRVIPA
                     DNILFASEMVGAVKGIDPRTGHYYDDTKRYIDQVSWLTDADKHKIFESNARKVYPRLN
                     KRLAASAAS"
BASE COUNT          309 a          471 c          531 g          368 t
ORIGIN      
        1 ctcgaaggac ggccgcaggt cgagatcgtg tccgtgggca tcccaccttc gagagcatca
       61 cgtccgccgc gtttaagggt gcggctccat taggattgct tgcagaggga gcaatcgtca
      121 tttgcgtcga caattgtttc agcggctccc ggccaagtcc gcccacgcgc tcgcgcacaa
      181 gcctttcgaa gcgcttcgcg acgatccgcg acagcggcgg ggatatttcg cctatcgtca
      241 tgtggggacc acgcattgcc ctcaagacgg ggtttagcca gccaagcttg tttcgaggcc
      301 tggtctacgc aattgcttat ttcgagacat cgctggcccg tcgcccacct tgctgcagtc
      361 gcagtcgata gcgaaaccca tcaaagcttg ccggggcgaa aacgatggtt ggttctcgcc
      421 cagttttatc gggccggccg gcgcggctca aaccactcta cgaggcggcg ctcgccgcta
      481 ggcgcttatt gagacgagga tacaccttgc gtgcgttgct ctcgaaaatc ttatgcttgt
      541 ctgcgtcggt cagccagctc acctgatcga tgtagcgttt ggtgtcgtcg taatagtgtc
      601 cggtgcgcgg atcgatgccc ttcacggcgc cgaccatttc ggaggcgaac aggatattgt
      661 cggccgggat cactctggtg agcagttcga tcccaggcaa atggtagacg caggtgtcga
      721 aaaaaacatt gttgagcaag tgctcttcaa gcggcggccg cttcatgtcc tgggcgagac
      781 ctcggtaacg gccccagtga tacggcaccg ccccgccgcc atgcggaacg atgaatttca
      841 gggtcgggaa atctttgaac agatccgacg tgatgagctg cataaaagcg gtggtgtcgc
      901 cgttgatgta gtgcgccccg gtagtgtgga acgccggatt gcacgaggcc gagacgtgga
      961 tcatggccgg cacgtcgagc tccgcgattt tctcgtagag cggataccag aagcggtcgc
     1021 tgatcggcgg cgcgtcccag tgcccgcccg atggatccgg attgagattg cagccgacga
     1081 agccgaactc gttgacgcag cgttccagct ccccgataca gttcttagga ttgactccgg
     1141 gcgattgcgg caattggcat acaccgatga agttgttcgg gtacagcgtg gtgaggcgat
     1201 agatcagctc attcgatacc tgcgaccatt cgaggctcac gccttcgtta ccgatatggt
     1261 gcgccatgcc ggatgcgcgc ggtgagaaaa tcgtaacatt ggtgccgcgc tctcgctgga
     1321 atttgagctg ggcgccttcc acgctcccac gcagctcatc gtcgctgatg ttgagcgtgg
     1381 cgcgcgacgg cttctggccg ggatccttga tggcggcgat ctggttcttg cggaaatcgt
     1441 gcagcttttg cggctccgtc gtgtagtgac cgtggcagtc gatgatcatg tcgcctcgct
     1501 gactgtcgag atatccgggg tgcccgtcgt cgccgaaagc ttaaggagag caatgcgatt
     1561 gtcaaacgga tcggtgggtt gagcggcgag aggacccggg gcggcgtcgc ggtgttgatg
     1621 acgtgctccc ggggtttcgc tcgatcaacc cggactctta tttggtttcg cgcagagcg
//
LOCUS       DATAQL010000364         3978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5226940, whole genome shotgun sequence.
ACCESSION   DATAQL010000364 DATAQL010000000
VERSION     DATAQL010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5226940"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VHS97_06265"
     CDS             complement(<1..732)
                     /locus_tag="VHS97_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3447838.1"
                     /translation="MHRGRRGQWFLPMVLAIAQGSAVNSAPPTGPLPGARESQAKTST
                     ARAGAGPSADVPDVNPLPSGPSAGGLGLGAPAALPASEGSRIFANPPGEEVLRGLPIN
                     LASALQLAGVSPLDIAAATVQVQQGLAVLLQAKALWIPTLNAGVDYQRHDGVQQNIFT
                     GALFQKGRQSLFVGGGPSLNVGVTDAVYAPLAARRVVAAQTANVQTARNDVLLQVSQA
                     YFTLQDARGRLAGVDATIERARRLVN"
     gene            complement(848..3031)
                     /locus_tag="VHS97_06270"
     CDS             complement(848..3031)
                     /locus_tag="VHS97_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX3447839.1"
                     /translation="MSAAPSLAPTLPFGQAAEHLVVKLRPDLVVQPQFYEGMTHYVIK
                     DPLALKYFRFKIEEYFLLQQFDGKQTLQDVKKAFERKYRPQTISIEDLTRFVAQLHEA
                     GIIQIDSPEQAKVLIRRRNKNRWRKIWAFLANILFIKIPIIDPERLLTRMYPFFRWIF
                     TRTFVTLSTLSMLLAIGLVASQWKTFYDKLPDFQSFFNWWTIASFWVCLAVVKIIHEF
                     GHGLTAKHFGGEVHEMGILFLVLTPALYCDVTDSWLLPNKWKRIWISAAGIYVECFLA
                     SIATFVWWYSTPGLLNSLAMATMFICSVNTILFNANPLLRYDGYYVLADWLEIPNLRI
                     KSTQFFGYLVQEKVLGLEIPVQSYLPRSRRILFVTYAVASYVYRWFVTFAILWFLSQF
                     LKPWKLESVSYLLAAGALVPLVGMPLYQIVKFLRTPGRLRKVKKARAAAFGVAAIALV
                     AGILLIPTPLRIQGTLVLKLAKPEEIYAEVQGRLVDLKVKNGQWVKKDTVLANLSNPE
                     KEKELSQRTQAYNISFHKALWYGQSGERDDRAQARQYEESAEKLSPMIEKVAEQLGKL
                     TLISHRDGQVIGLPHKETVGQWIKPGKPFCEIGDPHHLEAHLIVDQADINLISADRVA
                     WLKIYGRAETTYKSRVSEIAKRNSDEIPTELSNMAQGEVASKPDPKTGVAKPLTAVYE
                     VIIPIDNPELKLEPGLRGFAKIDGGTYTIAWWLKRWWNKLFNFQI"
     gene            complement(3253..3963)
                     /locus_tag="VHS97_06275"
     CDS             complement(3253..3963)
                     /locus_tag="VHS97_06275"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3447840.1"
                     /translation="MHREMAELTVKKAKLQADAIAPTVKAEAQVYVAASKVARDKRLN
                     EHGKGMVSAEDAAEHEGEYRVADAARREAKENQAIAKADLDLAERTLKEHIIVAPFDG
                     IIIKRMKHPGESVRANEAVVQLGNPNKLCANAYVPLDFAFRVKEGQIVEIQPRITIGR
                     GEPFPIEKKRFRGKITFIDPHIQPVAETAVRIRAEFENPNGDLRPGMKVQMTVFLTSD
                     VAAAGPETAAPTQTAHNK"
BASE COUNT          774 a         1155 c         1217 g          832 t
ORIGIN      
        1 attgaccagc cggcgggccc gctcaattgt cgcgtcgact ccagcgagcc ttcctctcgc
       61 atcctgcagg gtgaagtacg cctggctgac ttgcaacagc acgtcattcc gcgcggtctg
      121 cacattcgct gtctgcgcgg cgacgactcg ccgcgcggca agcggtgcgt aaaccgcgtc
      181 ggtaaccccg acgttcaagc tgggaccgcc gcccacaaaa agcgactgtc tgcccttttg
      241 aaacagcgct ccggtgaaga tattctgttg aacgccgtca tgccgttggt agtcgacgcc
      301 ggcgttgagc gtggggatcc acaaggcctt ggcttggagc aacaccgcga gcccttgctg
      361 cacctgcacg gtcgccgccg cgatatcgag tgggctcacg cccgccaact gcagcgctga
      421 ggcgaggttg atcggcagtc cacgcaacac ctcttcgccg ggcggattgg cgaagatgcg
      481 cgacccttct gaagctggca gtgcggccgg ggcacccaga ccgagcccgc cggcgctggg
      541 gccggagggc aacggattga cgtccgggac gtccgcgctc ggacctgccc ccgcgcgtgc
      601 ggtcgacgtc ttggcctggc tctccctagc accaggaaga ggcccggttg gcggcgctga
      661 gttgaccgcg ctgccctggg caatcgccag gaccatcggc aagaaccact gaccgcgacg
      721 tccgcgatgc atcatccccc ctcggcatca tggcagtttg ggaaaaaatg gcaaaccgtg
      781 cgtttaaacg gttgccgctc ttcccaatga tcccgaggtt cttatcggcc gtatcagtcg
      841 cgtcgattca gatctggaag ttaaacagct tgttccacca gcgctttagc caccaggcga
      901 tggtatacgt accgccatcg atcttggcaa agccgcgcag acccggttcg agcttgagct
      961 ctgggttgtc gatagggatg atcacctcat acacggcggt cagaggtttg gccacgccgg
     1021 ttttgggatc cggtttggag gcgacctcgc cctgggccat gttggagagc tcggtcggga
     1081 tttcgtcgct attccgcttg gcgatttcgg agacgcggct cttgtaagtc gtctcggctc
     1141 tgccgtaaat cttgagccag gctacgcgat cggcgctgat caagttgata tcggcctggt
     1201 cgacgatcaa gtgggcttcc aggtggtgag ggtcgccgat ctcacagaaa ggcttgcctg
     1261 gcttgatcca ctgaccgacc gtttccttgt gaggcagacc gatcacctgg ccgtcccggt
     1321 ggctgatcaa ggtcagcttg ccgagctgct cggcgacctt ctcaatcatt ggactgagct
     1381 tctcggcgga ctcttcgtac tgtctggcct gcgcgcgatc gtcccgttcg ccgctctggc
     1441 cataccacag ggccttgtga aaactgatgt tgtaggcttg ggtacgctga gaaagttcct
     1501 tttccttttc ggggttggac aaattggcca agaccgtgtc tttcttcacc cactggccat
     1561 tcttgacctt gagatcgacg agtcgccctt ggacctcggc gtagatctcc tcgggtttgg
     1621 ctagcttgag gacgagtgtc ccctgaattc ggagcggcgt cggaatcaag aggattccgg
     1681 cgacaagcgc gatcgccgcc acgccgaagg cggccgcacg agcctttttc actttgcgca
     1741 acctcccagg ggtgcgaagg aatttgacaa tctgatagag cggcattccc accaagggga
     1801 caagagcgcc ggcggccagc agataactga ccgactcaag cttccacggc ttcaagaact
     1861 gcgacaggaa ccagaggatc gcgaacgtca cgaaccatct gtaaacatag ctagccacag
     1921 catacgtcac gaacaggatg cgccgcgacc gcggcagata gctctgaacc gggatttcga
     1981 ggcccagcac cttttcctgg acgagatagc cgaaaaactg cgtgctcttg atccgcaggt
     2041 tggggatctc gagccagtcg gccagcacgt agtagccgtc gtagcggagc aagggattgg
     2101 cgttgaacag gatcgtattg accgagcaga tgaacattgt ggccatcgcc agactgttta
     2161 gtaatcccgg cgtgctgtac caccagacga aggtcgcgat gctggccagg aagcactcga
     2221 cgtagatccc ggccgcggag atccagattc gcttccactt gttgggcaag agccaactat
     2281 cggtgacgtc gcaatacaag gcaggggtga gcaccaggaa caagatcccc atttcgtgaa
     2341 cttcgccacc gaagtgcttt gcggtcagac catggccgaa ctcgtggata atcttcacga
     2401 cggccaggca aacccagaag ctggcgatgg tccaccagtt gaagaagctc tggaaatcgg
     2461 gaagcttgtc gtaaaacgtt ttccactggc tcgccaccag tccaatggcc aggagcatcg
     2521 acagcgtgct caaggtcacg aacgtacgcg tgaagatcca gcggaaaaag ggatacatcc
     2581 tcgtcaagag acgctcgggg tcgatgatgg ggatcttgat gaagaggatg ttggcaagga
     2641 atgcccagat tttacgccag cggtttttgt tgcggcggcg gatcaaaacc ttcgcctgct
     2701 cgggactatc gatctggatg atgccggctt cgtgaagctg cgcgacgaat cgcgtcaggt
     2761 cttcgatcga aatggtctgg ggccggtact tcctctcgaa ggcttttttg acgtcctgga
     2821 gagtctgctt gccgtcgaac tgctggagta gaaagtactc ttcgatcttg aagcgaaaat
     2881 acttaagcgc gagtgggtct ttgatgacat agtgcgtcat cccctcatag aactgaggct
     2941 gcacgaccag gtcggggcgc agcttgacga ccaggtgttc ggcggcctgt ccaaaaggca
     3001 acgtgggggc cagggaggga gcggcgctca tgagtcgtca accctttgga tcgtcgtcca
     3061 ctgatccgtc aatcttcgat tgacgggggg aacggtcgga gacctgcagt cggcgggggc
     3121 ggggtcagtc aggagacccg cgcccaacaa tcgtcgtcaa cccttcggaa cgtcgtacgg
     3181 gtcaagagcc gggtatcggg gcggagcgta ctagaaagca cgccctgccc cgaggcccca
     3241 aatcaaggtc acttacttgt tgtgtgccgt ctgcgtcggc gcggccgttt caggtcccgc
     3301 cgccgcgacg tcgctggtca agaagaccgt catctgcacc ttcatgccgg gccgcaggtc
     3361 accgttggga ttttcaaact cggcccggat gcggacggct gtctcggcga caggctggat
     3421 atggggatcg atgaaggtaa tcttgccccg gaatcgcttt ttctcgatag gaaaaggctc
     3481 gcctcgcccg atggtgatac gcggctggat ctcgactatc tggccctcct taactcgaaa
     3541 ggcaaaatcg agcggaacat aggcgttggc gcacaattta ttcggattgc caagctggac
     3601 gaccgcctca ttggcgcgaa cactctcgcc ggggtgtttc atgcgcttga tgataatgcc
     3661 atcgaagggg gcgacgatga tgtgctcttt taaggtccgt tcggccaggt cgagatcggc
     3721 cttggcgatc gcttggttct ccttggcctc tcgccgcgcg gcatcggcga cgcgatattc
     3781 gccttcgtgc tcggcggcgt cttcagcaga gaccattccc ttgccgtgct cgttcaaccg
     3841 cttgtcgcgt gccactttgg aggcggcgac gtagacctgg gcctctgctt tcacggtggg
     3901 ggcaatggcg tcggcctgga gcttggcttt tttcaccgtc agctcggcca tctcgcggtg
     3961 caagaagccg attgttcc
//
LOCUS       DATAQL010000365         5313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5074726, whole genome shotgun sequence.
ACCESSION   DATAQL010000365 DATAQL010000000
VERSION     DATAQL010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5074726"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VHS97_06280"
     CDS             complement(<1..377)
                     /locus_tag="VHS97_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX3447841.1"
                     /translation="MPAVSQPTSPGPLHLSKRVLMGPGPSDVHERVLEALATPLVGHL
                     DPEFVALMDETQDLLRYVFQTQNRLTLAISGTGSAGMEAVVVNLIEPGDRMLICINGV
                     FGARMQDVAERAGAKVTTIERPYG"
     gene            complement(451..1023)
                     /locus_tag="VHS97_06285"
     CDS             complement(451..1023)
                     /locus_tag="VHS97_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX3447842.1"
                     /translation="MRLTQRLTGRNGALLVVDLQDKLLESIPNREPVVANVVHLIRGA
                     GVLGLPVWATEQYPRGLGPSTARVAELIPDRPAKMAFHCCAVPQILEQLYGRNVRHVT
                     VAGIEAHVCVSQTALELLDLGFRVQVPADAVASRHKVDWEFALRRLEQAGAVVSTTES
                     VLFEWTETADRPEFKAISELIKTMNRPPVH"
     gene            1493..3274
                     /locus_tag="VHS97_06290"
     CDS             1493..3274
                     /locus_tag="VHS97_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3447843.1"
                     /translation="MTVRTISKALALLRDQSFDAVYVDASQLSAVRWVGMMIQAEEIL
                     DAISDGVAVVDPDLRIIWANPEFHLLTDSATQVVGCPFYQALGQPEILGPEPCPFTSA
                     VATKAPASTAVKIKGNRYLRLTVTPVFDATSALTHLIALTRETTDETQQQLKVDAIHE
                     AGDQLADLSPEELAEMAVEERTDLLKYNIGRHMKDLLGLDFIEIRLLDRPTNRLIPLL
                     TEGMTPLAANRELYARKEGNGVTGFVAATGLSYVCPDTTKDPLYIEGAAGSRSSLTVP
                     LIIHGTVIGTLNVESPQPDAFDDRDRQFIEIYGRNIAAALNTLELLQAEKESTATASV
                     EAINRELALPLDDIITDATTVLDRYAGHDEDIIARLRHLLYRAREIRSLIHKAGSTVV
                     PQPKQSRAVPAPRLAGNRILVVDADEAVRRSAHHLLGCQGASVETARDAHEAIALMRQ
                     APYSAALVDIRLPDLDGYESFKRLREVQPNVPVILMTGFGWDPSHSIVKARQEGLQTV
                     LYKPFRADRLMDAVEQALRTTPAPRQTAAPHQDGAHSPGPALPLSPSSPPNSSAGDLP
                     HPGAASQTPIDQKPSDSQPIEASNHES"
     gene            3264..4490
                     /locus_tag="VHS97_06295"
     CDS             3264..4490
                     /locus_tag="VHS97_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3447844.1"
                     /translation="MSLDWPWPVLLALVIPPALGHLYHFVLVVNWGSGLGYREPAMDR
                     IREVLLSAFLVSSALLLWMHLRTPWWTWSWPLWSYAVLCVLSGTVIGPFNSWRIIRRR
                     IPSGIHGSSVTLDLAQQDGTTSLIGHSRCSRLLRLPRNQSFQLHLREWDVAIPDLPQE
                     LEGLRIVQLSDLHIARCYDRRFFEAVVNSCLGWSADLVVMTGDLVEDDDTVGWIEPIL
                     RPLEARLGKFAILGNHDNDHQPHIIARELDRAGFELLEGRWTTLDLDGTTLAIGGTSE
                     PWGPALTTGEMPTADFRILLSHSPDLFHKAQRWGVNLMLSGHNHGGQIRFPLIGPVFM
                     PSRYSRRFDRGFFRQNETLLHVSEGVGGKHPVRYGCPPEVSRLVLRTAKGSLGSDGKT
                     AHSEQRQAMELDWVES"
     gene            4568..5269
                     /locus_tag="VHS97_06300"
     CDS             4568..5269
                     /locus_tag="VHS97_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3447845.1"
                     /translation="MPSPFPGMDPYLERPGLWRDLNHSHISTIQGLLAAQLRPTYVVR
                     IDERVYIVEECHGASKLQLSDRFEHEVREAFLKIIDRETREVRTVIEVLRPTSKAMGS
                     PGLRNLKLHRDDVMGSSSHWVEIDLFRGDRTVDVPTSVGPHEYVVHVSKRDMRPRAML
                     YPIRFWQRLPIIPIPVRTGDPDASLDLQAALDAIYDRAGYDLDIDYRREPNPPLSAAF
                     AAWADQLLRAKGIRS"
BASE COUNT         1026 a         1690 c         1527 g         1070 t
ORIGIN      
        1 ccatagggtc gctcgattgt cgtgactttg gcgccggcgc gctcggcaac gtcctgcatc
       61 cgcgctccga agacgccatt gatacaaatc aacatccggt cgcccggctc gatcagattg
      121 acgaccaccg cctccatacc ggccgagcct gtccccgaga tcgccagcgt cagccggttc
      181 tgggtctgaa acacatagcg cagcaagtct tgggtctcgt ccatcagggc cacgaactcg
      241 gggtcgaggt ggcccacgag cggcgttgcg agcgcttcca ggacgcgctc gtgcacatca
      301 ctgggaccag ggcccatcaa gacgcgcttc gaaagatgaa gtgggccggg cgacgtcggc
      361 tgtgacacgg cgggcattaa ccagactcct tggtggaaag acgcgtcaaa tcgggaatcg
      421 accaacccaa tatgactttg acatcggcga tcaatgaacc ggtggccggt tcattgtttt
      481 tatcaattca ctgatcgctt tgaactcggg tcgatcggcg gtctcggtcc attcgaagag
      541 aaccgactcg gtggtggaaa cgaccgctcc ggcctgttcc agccggcgca aggcaaactc
      601 ccagtcgacc ttgtgtcgcg aggctaccgc gtcagccggc acctggaccc tgaaccccag
      661 atccagtaat tcgagcgccg tctgggagac gcagacatgg gcctcgatcc ccgcaacggt
      721 gacatggcgg acattccggc cgtagagttg ctcgagaatc tggggaaccg cgcagcaatg
      781 aaaggccatc ttggcgggcc gatccggtat gagttccgcc accctggccg tgctcggtcc
      841 caggccgcgg ggatactgct ccgtggccca cacaggcaaa cccagcactc ccgcgcctcg
      901 aatcaggtgg accacatttg cgacgacagg ctcacggttc ggaatcgact cgagcagctt
      961 gtcttgaagg tcaacgacca agagtgcacc attccgaccg gtcaatcgct gagtcagacg
     1021 cattcaagca ccccgcctac cgaaacctac ggcaatcgac cgaaaaaact ccggccatcc
     1081 atccgctgcg atgctaaggg ccgtttcacc cggctgtcaa gcaattccac gaccttgaaa
     1141 tcgacgctcg caaaacgacc gcgctcgctc ctgtgcgctc cgttccttgc ccgaatttct
     1201 cggagcgcgc aatttgaccc gattgtccac gtgcttgcat ggccagtatt ttacctgtcg
     1261 atacgtttca aggggtgtct tgccgtgctg atacgaccgg gttaccatca atttgaaccg
     1321 tgttaggttt cctctacgga gatcggctcc gtgagctcgc gcgactgcag ggcgtgcaac
     1381 gtccaagcta ggggtgaccg gtgtggccga aaagcctcga cttttggtca tagatcgaga
     1441 cagctcgcat ggaaagtcga gcgttgactt tctgtctgag tcgtttacgc ccgtgacggt
     1501 ccgcacgatc tccaaggcgc tcgcgctttt gcgcgaccag agcttcgacg ctgtctacgt
     1561 tgatgcctcg cagctctcgg cggtccgttg ggtcgggatg atgatccagg ccgaggagat
     1621 cctcgatgcg atctccgatg gcgttgctgt ggtcgacccg gatttgagga ttatctgggc
     1681 caatcccgag tttcacttac tcacggactc ggcgacgcag gttgtcggct gtccgtttta
     1741 tcaggccctc ggccaacccg agatattggg ccccgagccg tgcccgttca cgtcggccgt
     1801 ggccacgaaa gcgccggcca gcacggcggt gaagatcaag gggaatcgct atctccggct
     1861 cacggtcacg ccggtctttg acgcaacgag tgcactgaca cacctgatcg cactgacccg
     1921 agagacaact gacgagaccc agcaacagct caaggtcgat gccatccatg aagccggcga
     1981 ccagctcgcc gacctgtcgc ccgaggaact cgccgagatg gctgtcgaag agcggaccga
     2041 cctgctcaag tacaacatcg ggcggcacat gaaagacctg ctgggcctcg attttatcga
     2101 gatccgccta ctcgatcgtc cgaccaaccg cctgatcccg ctcttgaccg agggaatgac
     2161 tcctcttgcg gccaaccgtg agctctacgc ccgcaaggaa ggaaatggcg tcaccggttt
     2221 tgtggcggcg acggggcttt cttacgtgtg tccggacacg accaaagatc ctctctacat
     2281 cgagggagcc gcgggttcac gcagctcgct gacggtccca ctcatcatcc acggcacggt
     2341 catcggcacg ctcaacgtcg agagccctca gcccgacgcg tttgatgacc gcgaccgcca
     2401 gttcatcgag atctatggac gcaacatcgc ggcggccttg aatacgctcg agttgcttca
     2461 ggccgagaag gaaagcactg ccaccgcatc ggtagaagcg atcaaccgcg agctggcgct
     2521 gccacttgac gacatcatca ccgacgcgac caccgttctg gatcggtacg ccggccacga
     2581 cgaagatatc atcgcgcggc tgcgacacct gttgtaccga gcgcgtgaga ttcgcagctt
     2641 gattcacaag gccggctcca cagtcgtacc ccagcccaag cagagccggg ctgtccccgc
     2701 gcctcggctg gcgggcaacc gcatcctggt cgtcgacgcc gatgaggccg tccgcaggtc
     2761 ggctcatcac ctgttaggct gccaaggcgc gagcgtcgaa acggcccgtg atgctcacga
     2821 ggcgattgcc ctgatgcggc aagctcccta ctcggccgcc ctggtcgata tccgcctgcc
     2881 agaccttgat ggctatgaat ccttcaagcg cctgcgcgag gttcagccca acgtacctgt
     2941 catcctcatg accgggttcg ggtgggaccc ttcccactcg atcgtgaagg cgcgtcagga
     3001 gggccttcag accgttcttt ataaaccatt ccgggcggat cgtttgatgg atgccgtcga
     3061 acaagccctc cgcaccacgc ccgccccgcg gcaaaccgcg gccccgcacc aggatggcgc
     3121 gcactctccc ggtccagcct tgcctctctc gccatcctcg ccgccaaact cttccgccgg
     3181 cgaccttccc cacccaggcg ctgcctctca aactcccatc gaccaaaagc cctctgactc
     3241 acaaccgatc gaggcatcca atcatgagtc ttgactggcc ctggccggtc ctgctggcgc
     3301 tcgtaatacc gccggccttg ggacatctct accacttcgt tctcgtggtc aactggggca
     3361 gcggtttggg ctatcgcgaa ccggcgatgg accggatacg cgaagtcctc ttgagcgcgt
     3421 ttttggtctc ctcggccctt ttgctctgga tgcacctgcg cacaccgtgg tggacttggt
     3481 cgtggccgct ctggagctat gccgtcctgt gcgttttgtc gggcacggta atcgggccat
     3541 tcaactcatg gcgaattatc cgccggcgca ttccctcggg catccatggt tcgtccgtga
     3601 ccctcgatct ggcccagcaa gacggaacga cctccttgat cggtcacagt cgatgttccc
     3661 ggctgctcag gctgccgcgc aaccagtcgt tccagttgca tctacgcgag tgggacgtcg
     3721 ccattcccga cctgcctcaa gagctcgagg gtctgcggat cgtgcagttg agtgacctcc
     3781 acatcgcccg ttgctatgac cgccggttct ttgaagccgt ggtcaatagc tgcttgggct
     3841 ggagcgccga cctggtcgtc atgacgggcg acctggttga ggacgatgac accgttggct
     3901 ggatcgagcc aatattgcgc ccacttgaag cgcggctggg caagttcgcg atcctgggca
     3961 atcacgacaa cgaccatcaa ccgcacatca tcgcgcgcga gctcgaccgc gcggggttcg
     4021 agttgcttga aggacggtgg acgacgcttg acctcgatgg gacgacgctg gcgatcggcg
     4081 ggacttcaga accctgggga ccggcactca caaccggtga aatgccaact gccgacttcc
     4141 gcattttgct ctcccattcg cccgacctct ttcacaaagc ccagcgctgg ggggtcaacc
     4201 tgatgctctc gggacacaac cacggtggcc agatccggtt tcccctgatt ggaccagtct
     4261 tcatgcccag ccgctattca cgccggttcg accgcggttt cttccgccag aacgagacgc
     4321 tgctccatgt cagcgagggg gtggggggca aacacccggt tcgttacggg tgcccgccgg
     4381 aagtcagccg gttggtgctc cgcacggcga agggtagcct cggaagcgac ggtaagaccg
     4441 ctcacagcga acagcgacaa gccatggagc tggattgggt tgagagctga cgcttcgcta
     4501 gagttttgtt ggtgagaatg ttccgacggc aaagcctatt gctttctccg ctgtgaggat
     4561 ctcgacgatg ccttcaccat ttccgggcat ggatccttat ctggagagac cgggcctatg
     4621 gcgcgatctc aaccacagcc atatttcgac gattcaaggt ttactcgcag cgcaacttcg
     4681 gcccacgtat gtcgtcagga tcgatgagcg ggtctatatc gtggaggaat gtcacggcgc
     4741 ttccaaactg caattgtccg atcggttcga gcacgaagtt cgcgaagcgt ttctgaaaat
     4801 catcgatcgc gagacgcgcg aggttcggac cgtcatcgag gttctgcggc caacaagcaa
     4861 agcaatgggt tcgcccggac tcaggaattt gaagctgcat cgcgacgatg tcatgggttc
     4921 ctcgagtcat tgggtggaaa tcgatctgtt tcgcggagat cgaacggtgg acgtcccgac
     4981 cagcgttgga ccccacgaat acgttgtcca tgtgtcgaag cgagacatga gacctcgagc
     5041 tatgctctat ccgatccgct tttggcagcg gctgcccatc attccgatcc ccgttaggac
     5101 cggcgatccg gacgctagtc ttgacctcca agctgcgctc gacgcgatat atgatcgcgc
     5161 gggctatgac ctcgatatcg actatcgacg cgagccaaac cctccgctca gtgcagcttt
     5221 cgccgcgtgg gctgatcagc tcttgcgagc caagggcata cgctcttagt gtcgtgtagc
     5281 tccactccag ctttcgtcgc tgcttcccaa tat
//
LOCUS       DATAQL010000366         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5379778, whole genome shotgun sequence.
ACCESSION   DATAQL010000366 DATAQL010000000
VERSION     DATAQL010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5379778"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3207)
                     /locus_tag="VHS97_06305"
     CDS             complement(<1..>3207)
                     /locus_tag="VHS97_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447846.1"
                     /translation="DLLAATAALQYVKTSRATAAPEAARLTQRIRGFVSALIAAQNED
                     GGWPWVSGGPLPRSSGKQPLAASDRLASAAVVWALASAQPLGLVTDVKVLDHAGAYLS
                     QEFSKTSGNDQETRAALLHALSTRRAASFEAANSLNRARNGLSDPALAYLALTFANLD
                     RGTLANELIGILASRAKSEATAPGHPSRVYWTNSGRSQAIRGATETTALVALAFSKVR
                     PQAPELDGAVAWLVAHRAGAGWQPYKAKGPALAALATYYGRAQRAEDRYRLTVTVNET
                     QVAQLNVAGTADGQVIAVPTKALKVGQPNRVRFAMEGRGRFGYAINMAGFTRDLKPDQ
                     IATNRVASINRRVYFPAAPELDGKVLPVGFGAAVNAPFFENLASQVALGGKARVAITA
                     WRTIPATTPEWERDFLIVEEHLPAGTTLIEGSVHAAATSYNLADGMLTFYFAPNQNPG
                     LITYDVHGYLPGQYRALPTSIRSAYEPGRFHLGPASELKVRAPGEPNTDPYKPTPDEL
                     YARGKAHYAAGRFTEAGEALEPLFGGYTLREDVAKDAARMLLLVSIHEDQARKIVQYF
                     EVVKEKAPELVLSFDHLLAIGKAYRDINEYERAMIVWRGLIEASYLEDARVGELLRQR
                     GKTLESIAYLIELWRSYPNTASIESDFFGLSQVLTQTASQAFTAPGLRRELAVAGVTR
                     SELLLQSIRMIQVFLSQSPNNPVADEASLSLVGAFTELEDFKAVDKLASRFAKLYPKS
                     TYLDSFQYSEALANFHLGQYDRAIEVAQTIARAVYKDAAGVDQPSPNKWQAIYILGQI
                     HDARRQPAKALEYYRQVADRFTDAAGAIQSYTRKDLKVPEVSVVKSDVTPVVAAGPPA
                     LEPGRQGLRAIDVSAAGTTRPTAPASTPGIGLDYRNIGQVDVKVYPVDLMQLYLTRRN
                     LDGIAGIDLAGVTPLVEKTVALGDGADYDDKSRSIDLPLSKEGAYLTMIRGDNLYASG
                     IVLVTPLEMEVLEEPAGGRVRITVRDSRSKEFVPKVQVKVIGSDTAQFISGETDLRGV
                     FVAEGLRGLVTAVARKANSQYSFYRGKTYLGQAAAPPAV"
BASE COUNT          571 a         1000 c         1047 g          589 t
ORIGIN      
        1 gaccgccggg ggcgcggcag cttgtcccaa ataagtcttg ccgcgataaa acgaatactg
       61 cgaattcgcc ttgcgggcga ccgcggtgac cagccctcgt aggccttcgg caacaaacac
      121 cccccgtagg tctgtctcgc ccgagatgaa ctgagccgta tcgctgccga tcaccttgac
      181 ttgcactttc gggacaaact ctttcgagcg actatcgcga acagtgatgc ggacacggcc
      241 gccggccggc tcttcgagca cctccatctc cagcggagtg accagcacga ttcccgaagc
      301 atagagattg tcgccccgga tcatggtcag gtaggcacct tccttggaca acggcaggtc
      361 aatcgacctg gacttgtcgt cgtagtcggc gccatcgccc agcgcgaccg tcttttcaac
      421 cagcggtgtg acaccggcca ggtcgattcc ggcaatcccg tccaggttgc gccgggtgag
      481 ataaagctgc atgaggtcga ccgggtaaac cttgacatcg acctggccga tattgcgata
      541 atccagcccg attccgggcg tggacgccgg cgccgtcgga cgagtagtac cggcggcgct
      601 cacgtcgatc gcacgtagac cctgccggcc tggctcgagg gcaggggggc cggccgccac
      661 gaccggcgta acgtcggact tgacgaccga tacctcaggc accttcagat ctttacgcgt
      721 gtatgactgg atcgcgccgg cggcgtcagt gaaacggtcg gccacctggc ggtagtattc
      781 cagagccttg gccggctgac ggcgggcgtc gtgaatctgt cccagaatgt aaatcgcttg
      841 ccacttgttg gggctgggct ggtcgacacc ggcggcgtct ttgtagaccg cgcgggcgat
      901 cgtctgcgcg acctcgatcg cgcggtcgta ctgcccgagg tgaaaattgg cgagtgcctc
      961 gctgtactga aaactgtcga gatatgtgct cttgggatag agtttggcga accgggacgc
     1021 cagtttgtcg accgccttga aatcctcgag ctcggtgaac gctcccacga gcgagagact
     1081 cgcttcgtcg gccaccgggt tattgggtga ctgcgacaga aagacctgga tcatccggat
     1141 cgactgcagc aacagttccg accgggtcac tcccgcgaca gccagctcgc ggcgcagccc
     1201 gggagcggta aacgcctgcg acgctgtctg ggtcaagacc tgagagaggc cgaaaaagtc
     1261 actctcgatc gaggccgtgt tggggtagct ccgccagagc tcaatcagat aggcgattga
     1321 ttccaacgtc ttgccgcgct ggcgtaaaag ctcgccaacc cgtgcgtctt cgagatagct
     1381 ggcctcgatc aggccgcgcc agacgatcat cgcacgctca tattcgttga tgtcgcgata
     1441 agccttgccg atcgccagca agtggtcgaa gctgagcact agctcaggtg ctttttcttt
     1501 gacaacctcg aagtactgga cgattttgcg agcctggtct tcgtggatgc tcacgagcaa
     1561 gagcatgcgc gcggcgtcct tggcgacgtc ttcgcggagc gtgtatccgc cgaacaaggg
     1621 ctcgagcgcc tcgccggctt cggtgaagcg gccggcggca tagtgggcct tgccgcgggc
     1681 gtaaagctcg tcgggcgtgg gcttgtaagg atccgtgttc ggctcgcccg gcgcccgcac
     1741 cttgagctcg ctcgccggac cgagatggaa tcgccccggc tcgtaggcgc tcctgatcga
     1801 cgtcggcaag gcgcggtact gccccggcag gtagccgtgc acatcgtacg tgatcaaccc
     1861 cgggttctgg ttgggagcga agtaaaaggt caacattccg tcagcaaggt tgtacgaggt
     1921 cgcggccgcg tggacggagc cttcgatcaa tgtggtgccc gccggcagat gctcttcaac
     1981 gatcaagaag tcgcgctccc attcgggcgt cgtggcgggt atggtccgcc aagccgtgat
     2041 ggcgacgcgg gctttgcctc ccagcgcgac ctggctggcc aggttttcaa agaagggggc
     2101 gttcaccgca gcgccgaaac ccactggcaa taccttgcca tcgagctcgg gtgcagccgg
     2161 aaaatagacg cggcgattga tcgacgcgac acggttggtc gcgatctggt cgggcttgag
     2221 atcgcgagta aatcccgcca tgttaatggc gtagccgaac cggccgcgtc cctccatcgc
     2281 aaaccgaacg cggttgggct ggccgacctt cagcgccttg gtggggacgg cgatgacctg
     2341 gccgtcggcc gtaccggcca cgttgagctg ggcgacctgc gtctcgttca cggtgacggt
     2401 caaccgatac cggtcttcgg cacgctgggc gcggccgtag taagtcgcca gcgcggcaag
     2461 cgccggcccc ttggccttgt aaggttgcca gccagccccg gcgcggtggg ccacgagcca
     2521 cgcgaccgcc ccgtcgagct caggcgcctg cggtcggacc ttgctgaagg caagggctac
     2581 cagcgctgtc gtttcggtgg cgccgcggat cgcttgcgag cggccggaat tcgtccagta
     2641 gacgcgcgat ggatggccgg gagccgttgc ctcgctcttg gcgcgcgagg ccaggatgcc
     2701 gatcagttcg ttggccagcg tccctcgatc gagattggcg aaagtgagcg ccaggtaagc
     2761 cagagcaggg tctgagagcc cgttgcgcgc gcgattcaag ctgttcgccg cctcaaagga
     2821 cgccgcgcgg cgcgtactga gagcgtgcag taatgcggca cgcgtctcct gatcgttgcc
     2881 gctggtctta gagaattcct ggctgaggta agcccctgcg tgatcgagca ccttgacatc
     2941 ggttacgagc cccagaggct gggcggacgc cagggcccag acgaccgccg ccgaggcgag
     3001 acggtcgctc gcggcgagcg gctgtttacc tgacgaccgg ggcaggggac cgccactgac
     3061 ccatggccac ccgccgtctt cgttttgcgc cgcgatcaac gcggatacaa acccacgaat
     3121 ccgctgcgtc agccgcgccg cctcgggcgc ggcggtcgcg cgggacgttt tcacatattg
     3181 caaagccgcc gtcgcggcta ataagtc
//
LOCUS       DATAQL010000367         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_647075, whole genome shotgun sequence.
ACCESSION   DATAQL010000367 DATAQL010000000
VERSION     DATAQL010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647075"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..814
                     /locus_tag="VHS97_06310"
     CDS             380..814
                     /locus_tag="VHS97_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3447847.1"
                     /translation="MKSSLLSVLMFSAVLWPATTFAQSAPDAMAKAVKADNMKWEPLG
                     DSAFEISILYTNPTTQATYLVIRGPGNQHVPRHWHSSNESITVVKGTFVVAHDGSDDK
                     TVLAPGGFAYMPAKMIHEAWTGDGGATYFITVDGKWDVNFVE"
     gene            complement(1083..>1672)
                     /locus_tag="VHS97_06315"
     CDS             complement(1083..>1672)
                     /locus_tag="VHS97_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX3447848.1"
                     /translation="ARGIIVSHETVRQWARKFGQQFANQIRRRLPQVGDKWHLDEVVL
                     KIAGVKHWLWRAVDQAGVVLDVLVQRRRNKQAAKRLLRKLLKKQMRPPRVMITDKLAS
                     YGAAKREVMPGIEHRQHKGLNNRAENSHQPTRRRERQMKQFKSAAQAQRFLSAHDQIN
                     NLFHLRRDHVTAAEHRASRSQKFQVWAEICSMAWQ"
BASE COUNT          302 a          454 c          539 g          377 t
ORIGIN      
        1 atccggctgt atgtggaatc actgtgggca gctttgccga tccccattag ggggcgcccg
       61 gcgtcatctt ggtgtcgagt ctcgttcgcg ccgacttttc cgttcgcggg ggatcaagca
      121 gaggcgacat gaggacctca cgaactggtg tcaccgcggt accgatggtt cgaatccggt
      181 ttcctccagc ggggagtcga ctgcgaacct gatttccggg cgaatcctat cgatggccgt
      241 cggggatttc tcgctacgcg atgaaagaga aacaccggcc gggggcagtc tacccagctt
      301 gcggtggcgc gggggtttcg aggctggcgc ttttataaca aattcagcta aggttaagac
      361 gccagcgagg gagacagtta tgaaaagcag cttgctcagt gtgctgatgt tcagcgccgt
      421 gctctggcct gccacgacgt tcgcccagag cgcacccgac gcgatggcaa aagccgtcaa
      481 ggccgacaac atgaagtggg agccgctggg ggatagtgct ttcgaaatat cgatcctcta
      541 caccaatccg acgacccaag cgacatatct ggtgattcgt gggccaggga atcagcacgt
      601 accacgacat tggcactcgt ccaatgagtc gatcactgtg gtaaagggca cgttcgtggt
      661 ggcccatgac ggaagtgacg acaaaactgt gctagcccct gggggttttg cctatatgcc
      721 cgcaaaaatg atccacgagg cgtggaccgg tgatgggggt gcgacttact tcatcacggt
      781 cgatggcaaa tgggatgtga acttcgttga gtaatcgagg gtgttccgat catacgccgt
      841 cccgttttgt ccgtatttgg gacgccgaga tgcgtatgga tttggacgcg ctcgatgccg
      901 ctcatgcggg cctcgccacg cggcgcaggg tggaaatgct gcggtcgtag ctgtccgcca
      961 attcctgcaa cgtagcgccc tgcgcgcggc gtctggtggc ctctttgtgc tgtgccggtg
     1021 gcaccgtcaa cttgtcaggt cgggttagtc ggttgcaggc gactggtagt gggcatggcg
     1081 gtttattgcc aagccatgct acagatttcg gcccagacct ggaatttttg gctcctggaa
     1141 gctctatgct cggcggcagt gacgtgatcg cggcggaggt ggaagaggtt attgatctga
     1201 tcgtgggctg agaggaagcg ctgcgcctga gcggccgatt tgaattgctt catttgccgc
     1261 tctcgtcggc gtgtcggctg gtgcgaattc tccgctcggt tattgagtcc cttgtgctgg
     1321 cgatgctcaa tgccgggcat gacctccctc tttgccgcgc cgtagctggc aagcttgtcg
     1381 gtgatcatga cacggggcgg ccgcatctgc ttcttgagca gcttgcgcag cagccgcttg
     1441 gcggcttgct tgttgcgtcg gcgttggacc aggacgtcga gcaccacgcc ggcctggtcc
     1501 acggctcgcc aaagccaatg ctttaccccg gcaattttga gcacgacctc gtccagatgc
     1561 catttgtcgc caacctgggg aaggcggcga cggatctgat tggcaaactg ctgaccgaac
     1621 ttgcgcgccc attgtcggac ggtttcgtgg ctaacgatga tgccgcgggc cg
//
LOCUS       DATAQL010000368        10356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3089425, whole genome shotgun sequence.
ACCESSION   DATAQL010000368 DATAQL010000000
VERSION     DATAQL010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089425"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..404
                     /gene="rpsS"
                     /locus_tag="VHS97_06320"
     CDS             120..404
                     /gene="rpsS"
                     /locus_tag="VHS97_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX3447849.1"
                     /translation="MGRSLKKGPYVVERLLEKVSRADSSGSREPIKTWARACTVVPEF
                     VGKHFAIHNGKIFVKLYVTEDMVGHKFGEFAPTRTFRGHGGKASKSAPKK"
     gene            487..879
                     /gene="rplV"
                     /locus_tag="VHS97_06325"
     CDS             487..879
                     /gene="rplV"
                     /locus_tag="VHS97_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX3447850.1"
                     /translation="MSLTAEYQATHRFARISVRKVRPLLDLIRGHYADDALDVLKYMP
                     HRGARLVEQVLKSAMANAEDKGIRNVGELVVTDARGDGGPMFKRLMPRARGMAYLIRR
                     RTAHIAVGLADMANWSANQEQGADQDDE"
     gene            953..1654
                     /gene="rpsC"
                     /locus_tag="VHS97_06330"
     CDS             953..1654
                     /gene="rpsC"
                     /locus_tag="VHS97_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX3447851.1"
                     /translation="MGQKVRPTGFRVGVMEDWRSRWYASKHEFADLLIEDFKIRAFIK
                     KKYGFAGIPKIEIERTRDAVTVLLSTARPGVIIGRKGAEVENLQKELQTQTGRKIEIK
                     IVEVARPEIDAQLISEDIAEQLQKRSSFRRTMKRAIEQTMDGGARGVKIQLSGRLGGA
                     EMSRTEATAAGSVPLSTLRARVDYGFAEAKTAQGHIGIKVWVNQGDYLKVEAGDGTDA
                     QAGQVPKKPKRPRKR"
     gene            1593..2012
                     /gene="rplP"
                     /locus_tag="VHS97_06335"
     CDS             1593..2012
                     /gene="rplP"
                     /locus_tag="VHS97_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX3447852.1"
                     /translation="MALMPKRVKYRKSQKGRVKGNATRGNYVSFGEYGLQTLEPGRIS
                     AQTIEAGRVVASQAVKGGGKVYIRIFPHKSVTAIPAETRMGKGKGEVEFWAAVVKPGT
                     ILYEVSGLSEEAARTVFARVAHKMPVTCRFVTRRTTV"
     gene            2027..2251
                     /gene="rpmC"
                     /locus_tag="VHS97_06340"
     CDS             2027..2251
                     /gene="rpmC"
                     /locus_tag="VHS97_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX3447853.1"
                     /translation="MSKPAELREQTEEQLNLLLTEVKHNLFRLRLQSETERLEAPSEI
                     VKAKREIARIKTILRLRQIEQEQSILAATP"
     gene            2332..2667
                     /gene="rpsQ"
                     /locus_tag="VHS97_06345"
     CDS             2332..2667
                     /gene="rpsQ"
                     /locus_tag="VHS97_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX3447854.1"
                     /translation="MSQVSPTSKKTTPARSRRKTEIGIVTSDKMSKTRRILVERLVPH
                     PKYGKMLKRRTVCHAHDEANSSHVGDVVEIMETRPLSKLKRWRIVRIVRPGAQQALAG
                     EDEAARTPS"
     gene            2794..3174
                     /gene="rplN"
                     /locus_tag="VHS97_06350"
     CDS             2794..3174
                     /gene="rplN"
                     /locus_tag="VHS97_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX3447855.1"
                     /translation="MIQMQTRLDVADNTGAKEVMCIKVLGGSASRYKRRTAGLGDTII
                     ASVKKATSGGDVKAGDVVRCVVVRTRNQTRRTDGSYVKFDRNAVVLIDAEKNPRGTRI
                     FGAIARELRDRQFMKIISLAAEVV"
     gene            3233..3622
                     /gene="rplX"
                     /locus_tag="VHS97_06355"
     CDS             3233..3622
                     /gene="rplX"
                     /locus_tag="VHS97_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX3447856.1"
                     /translation="MHIRKDDQVEIITGDDRGTPGDRKIAKVLRVIPERNKIVVEGVN
                     RVYKHLRPSQKNPQGGRLSKEMPIDVSNVLLYCSKCNRGVRIGHRFTDDGQKQRYCKA
                     CDVSLGNVGPKKPAHAVKARALAKKVL"
     gene            3649..4203
                     /gene="rplE"
                     /locus_tag="VHS97_06360"
     CDS             3649..4203
                     /gene="rplE"
                     /locus_tag="VHS97_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX3447857.1"
                     /translation="MARLQDQYKNDIAKALSAKFNFDNPMAIPKLTKIVINMGVGRAT
                     QDKALLESAVDSLSKISGQKPVITKAKTSVSGFRLREGNDIGCKVTLRGLRMYEFLDR
                     LISIALPRIRDFRGVNPNSFDGHGNFSLGLAEQVVFPEIDADKMQHAHGMDITIVTTA
                     TNDDQARELLHHFGLPFRQPVKAH"
     gene            4333..4518
                     /locus_tag="VHS97_06365"
     CDS             4333..4518
                     /locus_tag="VHS97_06365"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX3447858.1"
                     /translation="MSTKAQKIKSETPRKFAVQHRNRCRLCGRPRGFYRKFGICRICF
                     RNLALRGLIPGVKKASW"
     gene            4652..5047
                     /gene="rpsH"
                     /locus_tag="VHS97_06370"
     CDS             4652..5047
                     /gene="rpsH"
                     /locus_tag="VHS97_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX3447859.1"
                     /translation="MMTDPIADMLTRIRNAVRIERTILDMPASQMRRGIAQVLKDEGY
                     IWDYEEIETIPAKTLRLHMKYGPNGERLINRIDRVSKPGRRVYKGYRDLKPILGGMGI
                     QILSTPRGVLSDRRARTEKVGGEVLALIN"
     gene            5114..5665
                     /gene="rplF"
                     /locus_tag="VHS97_06375"
     CDS             5114..5665
                     /gene="rplF"
                     /locus_tag="VHS97_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX3447860.1"
                     /translation="MSRIGRKPVPVPTNVKIAIADSTIQVEGPKGKLSLTYRPEIDVK
                     FDQSGGQIVVTRHDDERTNRSLHGLTRSLVANMVQGVTSGYAKKLEIVGVGYQAQLKK
                     ANTVALQVGYANQVVLEAPPGVTVAVPDPTHVIISGADKQAVGQFAAVVRKVRPPEPY
                     KGKGIRYEGEFVRRKAGKAFGSK"
     gene            5706..6077
                     /gene="rplR"
                     /locus_tag="VHS97_06380"
     CDS             5706..6077
                     /gene="rplR"
                     /locus_tag="VHS97_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX3447861.1"
                     /translation="MANQQKLVQTRRQRRQHRVRNRMSGTLERPRLAVFRSSKHIYAQ
                     MVNDQTGTTLVSASTLDPEVKAQLKYGGNKAAATFVGRVVAERAKKAGIDKICFDRRS
                     YKYHGRVAALADAAREAGLKF"
     gene            6109..6606
                     /gene="rpsE"
                     /locus_tag="VHS97_06385"
     CDS             6109..6606
                     /gene="rpsE"
                     /locus_tag="VHS97_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX3447862.1"
                     /translation="MSSEGRERDRDRGEWTESVVSIRRCAAVVKGGRRFSFNALVVVG
                     NGRGQVAWGYGKANEVPPAVEKGVKDAHKQMKRVQLRGGTIPHAVIGQYGAAKVLLMP
                     ASPGTGVIAGGAVRAVVESAGVKDILTKSFGSNNKLNLVKAAIDALLKLRTKEEIARL
                     RGVPL"
     gene            6606..7115
                     /gene="rplO"
                     /locus_tag="VHS97_06390"
     CDS             6606..7115
                     /gene="rplO"
                     /locus_tag="VHS97_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX3447863.1"
                     /translation="MQLHDVHQGVHRRKKRKRIGRGIGSGHGKTSTKGHKGHSSRQGF
                     KLSPIFEGGQMPLARRVPKRGFVNGAFKKSFAIVNLADLDAKFEPGAVVDEKALRSKG
                     LVKGHAFDGIKILGDGALSKKLEVHATKFSGSAASKIAASGGKTVVVPYRPHAVHAQS
                     KEEKAGAVG"
     gene            7291..8664
                     /gene="secY"
                     /locus_tag="VHS97_06395"
     CDS             7291..8664
                     /gene="secY"
                     /locus_tag="VHS97_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX3447864.1"
                     /translation="MDKLFTIFKVPELRRKILFTALLLAIYRIGFYVPLPIVNQAQLK
                     NWEEQQSGNTAGKLFGTVAMFGGTSIGMSTIFGLGIMPYISASIIFQLLASVVPSLEA
                     MMKEGESGRKRINEYTRYATVVLCAIQSAFWVQYMMSPQMNVVLPDYRDFWHGMVSVC
                     IMTAGTIFLMWVGEQIDEYGIGNGISLLIMAGILARLPIAMKGLWENSTSKLTPEPGK
                     YGIITILLLLGLFIAVVVGVIAITESQRRIPTQSAKHVRGRRVFGGTKQFLPLKVNQA
                     GVMPIIFASSLLIFPSFVFSSLARATSGMRTDNWSVGSFFAGLFQEVADAFQRQSYIY
                     TVCYIILIYFFCYFWTAITFNPKDMAENLKDYGSFIPGYRPGRRTADYLEKVMLRITY
                     VGAAFLALVAVIPSLVQAALSVDWTVASFLGGTGLLIVISVCLDLVQKIDSHLVMRSY
                     TGLVSRR"
     gene            8772..9665
                     /gene="map"
                     /locus_tag="VHS97_06400"
     CDS             8772..9665
                     /gene="map"
                     /locus_tag="VHS97_06400"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX3447865.1"
                     /translation="MSSHRGLGSRPQMPALFSGLVRKPKAKGGANQTEAPPVIKLKSP
                     REIALMREAGRVVAKALDQVRRMAVPGVSTAEMNEAVTAIFAEHDAIPLFLGYPSSVK
                     GKPAFPAVICASVNEQVVHGIPNRRPLREGDIVSVDTGCKVNGWCGDAAVTLAIGTMR
                     PEIQKLLDVTSETLNLAIRAMARCGTWLEVASLMERFVKSQGLYVVEKFVGHGIGQTM
                     HEEPQVPNFVSQSLRKFDIKLEPGVVLAIEPMVALGTKDVRTLDDGWTVETKDRRFSA
                     HFEHTVAMTPEGPRVLTYLED"
     gene            9871..9984
                     /gene="rpmJ"
                     /locus_tag="VHS97_06405"
     CDS             9871..9984
                     /gene="rpmJ"
                     /locus_tag="VHS97_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX3447866.1"
                     /translation="MKVRSSVKRICENCKIVRRQGKVYVICTNPRHKQRQG"
     gene            10061..>10356
                     /gene="rpsM"
                     /locus_tag="VHS97_06410"
     CDS             10061..>10356
                     /gene="rpsM"
                     /locus_tag="VHS97_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX3447867.1"
                     /translation="MPRILGVDIPNDKRAVISLQYIYGIGPYLAGVLCERTGIDPEKR
                     ARDLSEDDLAKLASLLDNEYTVEGQLRRQVQQNIARLRDIGCYRGLRHRKGLPVR"
BASE COUNT         2253 a         2998 c         3198 g         1907 t
ORIGIN      
        1 ccgccgtagc gtccggtacg gccagctcaa agtgtgattg aatggttcga gatcatgaga
       61 caggcgacaa gatagcgcag agagtttgat tcgaccgact aaacaggacc ctatttgcca
      121 tgggacgttc gctcaagaaa ggcccgtacg tcgtcgagcg gctgctcgag aaggtgtcga
      181 gggccgattc ctccggtagt cgcgagccga tcaagacctg ggcccgggcc tgcacggtcg
      241 tgcccgaatt cgtggggaag cactttgcca tccacaacgg caaaatcttc gtcaagctct
      301 acgtgaccga agacatggtg ggccacaagt ttggtgaatt cgcccctacc cgaaccttcc
      361 gaggccatgg cggcaaggca tccaagtcgg ctcccaagaa gtgatcgtga tcgtatgtga
      421 aaagcggggg gacggccctc cgcttcgaat tccacccaat ttcaacggct gctcgagacc
      481 ctggccatga gccttacagc ggaataccaa gcgacacatc gttttgcccg gatctcggtg
      541 cgaaaggtcc gcccgctcct ggacctgatc cgtggccact acgccgacga cgcgctcgat
      601 gtcctcaaat atatgccgca ccgcggcgcg cggctggtcg agcaggtgct caaaagcgcc
      661 atggccaacg ccgaagacaa aggtatccgc aatgtgggcg aactggtcgt gaccgacgcc
      721 cgcggagacg gcggtccgat gttcaagcgg ctgatgcctc gtgcccgtgg catggcgtac
      781 ctgatccgcc gccgcacggc ccacatcgcc gtcggactcg ccgacatggc gaactggtcg
      841 gcaaaccagg agcaaggggc cgaccaggac gacgaatgat caaattacca gttacgaacg
      901 aggcgggaac acaatgagag tcggcacgaa acgagcaggg ggcgactgac acatgggcca
      961 aaaggttcga ccgaccgggt tccgcgtggg agtgatggag gactggcgca gccgctggta
     1021 tgcctccaag cacgaatttg ccgacctgct gatcgaagat ttcaagatcc gggcgttcat
     1081 caagaaaaag tatggatttg ccggaatccc caagatcgag atcgagcgga ctcgcgacgc
     1141 ggtgaccgtg ctgctgtcga cggcccggcc cggcgtgatc atcggccgta agggggccga
     1201 ggtcgaaaac ctccagaaag agctgcagac acagacgggt cggaagatcg agatcaagat
     1261 cgtcgaggtc gcgcggcctg agatcgacgc gcaattaatt agcgaggaca ttgccgagca
     1321 gctccaaaag cggtcaagct tccggcggac catgaagcgg gccatcgagc agaccatgga
     1381 cggtggcgcc aggggagtga agattcagct ttcggggcgg ttgggagggg ccgaaatgtc
     1441 acggaccgaa gcgaccgccg ccggctcggt tccattgagc acgctccggg ctcgggtcga
     1501 ctatggcttc gccgaggcca agacagcgca aggccatatc ggaatcaaag tgtgggtcaa
     1561 ccaaggcgac tatttgaagg tggaggcggg cgatggcact gatgcccaag cgggtcaagt
     1621 accgaaaaag ccaaaaaggc cgcgtaaaag gtaatgcgac ccgcggcaat tacgtgtcgt
     1681 tcggtgagta cggcctgcag acactcgagc cgggccgcat cagcgcgcag acgatcgagg
     1741 cgggtcgcgt cgtggccagc caggcggtga aggggggggg aaaggtttat atccgcattt
     1801 ttccccacaa gagtgtcacg gccattcccg ccgagacacg catgggaaag gggaagggcg
     1861 aagtcgagtt ctgggcggcg gtcgtcaagc cgggaacaat cctttacgaa gtgagtggcc
     1921 tctcggaaga agccgcccgc actgtctttg cccgcgtggc acacaagatg cccgttacct
     1981 gccgcttcgt cacccgccgg accacggttt aacgaggagt cgagtgatgt ccaagcccgc
     2041 cgaactgcgc gagcagaccg aagagcagct caatctgctg ctcaccgaag tcaaacataa
     2101 cctgttccgg ctgcgcctcc agagcgagac cgagcggctg gaggccccca gcgaaatcgt
     2161 caaggccaag cgcgagatcg cccgtatcaa gacgatcttg agactgcggc agatcgaaca
     2221 agaacaatcg atcctggccg cgacgccgtg acggcgcggc ggcttctatc gtttcgagcc
     2281 gctctagccc gagcgtttca ccccaatgaa cgaatcccat gagaactttc gatgagtcaa
     2341 gtaagcccga ccagtaaaaa gacgacgcca gcccgcagcc gccgtaagac ggagatcggg
     2401 atcgtcacgt ccgacaagat gagcaagacg cggcgcatct tggtcgaacg gctcgttcct
     2461 catccgaagt atggcaaaat gctcaagcgg cgcaccgtct gccatgctca tgacgaggcc
     2521 aactcatcgc acgtggggga cgtggtcgag atcatggaaa cccgccctct ttccaagctc
     2581 aagcgttggc ggatcgttcg aatcgttcgg cccggcgcgc agcaggccct ggccggcgaa
     2641 gacgaggccg cgcggactcc cagttgatga tgatactgaa attctgggcg gccgcgatct
     2701 ctcgagcgat tgccacgccg ccatgatggc tcgacgacaa acgcggccgc ggcaattagc
     2761 cccggccgcc gtgaatcaag ggtagagtgt cccatgatcc agatgcagac acggcttgat
     2821 gtggccgaca acaccggtgc caaggaagtg atgtgtatca aggttctggg cggcagtgcg
     2881 tcacgctaca agcggcgcac cgccggcctg ggcgacacga tcatcgccag cgtcaagaaa
     2941 gcgacttcgg ggggtgacgt caaggcgggc gatgtggttc gttgcgtcgt cgttcgaacc
     3001 cggaaccaga cccgtcgcac cgacggctct tacgtcaagt tcgatcgcaa cgccgtcgtc
     3061 ctgatcgacg ccgagaaaaa cccgcgcggc acccgcattt ttggtgcgat cgcccgtgag
     3121 ctgcgcgacc gccagttcat gaaaatcatc agcctggctg ccgaggttgt ttgagttatg
     3181 ggaaccgagt ggggtcgtca ccagtcaagg ccaatcaact agtgagctga tcatgcacat
     3241 tcgcaaggac gaccaggtag aaatcatcac gggcgacgac cgcggaacac caggggaccg
     3301 caagatcgcc aaggtactgc gcgtcattcc cgagcgaaac aagatcgtcg tcgagggggt
     3361 aaaccgggtc tacaagcacc tgcggccgag ccagaaaaat ccccagggcg gccgcctctc
     3421 gaaagagatg ccgatcgacg tgtccaacgt cctcctctat tgctccaagt gcaatcgtgg
     3481 agtccgtatc ggccaccggt ttaccgacga cggtcaaaag cagcggtatt gcaaggcgtg
     3541 cgacgtcagc ctcggaaacg tcggtcccaa gaagccggct cacgccgtca aggcacgggc
     3601 cctggcgaaa aaggttttat agcacttagt cgctgaagga taacgggaat ggctcgcctc
     3661 caggaccaat acaaaaacga catcgccaaa gcgttgtccg caaagttcaa cttcgacaat
     3721 ccgatggcga tccccaagtt gaccaagatt gtcatcaaca tgggcgtcgg acgggccaca
     3781 caagataagg ccctcctgga gtcggccgtc gacagcctga gcaagatcag cggtcagaag
     3841 ccggtgatta ccaaggccaa gacctcggtc tcgggcttcc ggctgcgcga gggcaacgat
     3901 atcggctgca aggtcactct acgcggcctg agaatgtacg agttcctcga tcgtttgatc
     3961 tcgatcgccc tgccgcgaat tcgagacttc cgcggagtga accctaatag cttcgatggc
     4021 cacggcaact tcagtcttgg actggctgaa caggtggttt tccccgagat cgacgccgat
     4081 aagatgcagc acgctcacgg gatggatatc accatcgtga ccacagccac caatgatgac
     4141 caagcccgtg agttgctcca ccacttcggc ctgccgtttc gccagccggt caaggcccac
     4201 tgatggagac gtgtcacgcg aaatgtttga gaacgcgagg tgatcggcaa ccgatcgcct
     4261 cgtgcacccg gcggcggatt gaaaacaaac aggagttcag gttgttcttt tccaggggtt
     4321 ggtgaaccga gcatgtcgac caaggcccag aagatcaagt cggagacgcc gcgaaagttt
     4381 gcggttcagc atcgcaaccg ctgtcgcttg tgcggacgtc cccgggggtt ctatcgcaag
     4441 ttcggcattt gtcgaatttg tttccgaaac ctggcccttc gggggctgat tcccggtgtc
     4501 aagaaagcga gctggtagtg ccgtggcaac atgccacggc cgttgttaat tcattgaatg
     4561 ttctgacccg ccgtgccgac gccaatctac cctcgatgtt tgtcgcattg actgggcgtt
     4621 acccgcgacg gtttttgaag gtctgttcgc catgatgacc gaccccatcg ctgacatgct
     4681 cacccgaatc cgcaacgcgg tccggattga gcgcacgatc ctggatatgc ccgcttcgca
     4741 gatgcgccgc ggtatcgcgc aggtgctgaa agacgaaggg tatatctggg attacgagga
     4801 aatcgagacc atccccgcca agaccctccg actgcacatg aagtacggcc ccaacggcga
     4861 acggctcatc aaccggatcg acagggtcag caagccgggc cgaagggtct acaaaggcta
     4921 tcgcgatctc aagccgatcc tgggaggcat gggaattcag attctgagca cgccccgagg
     4981 agttttgagc gaccgccgcg ctcgtaccga aaaggttggc ggcgaagtct tggcactcat
     5041 taattgaacc caagggtcgg cgattgatcg agattcttga tccgattcac cagatgcaga
     5101 ggacgacaaa ccaatgtcgc ggattggacg caagccggtg ccggtgccca cgaacgtgaa
     5161 aatcgcgatc gccgactcga cgatccaggt cgaaggaccc aaagggaagc tcagcttgac
     5221 gtaccggccc gagatcgacg tcaagtttga ccagagcggc ggtcagatcg tcgtcacacg
     5281 gcacgacgac gagcggacca accgttcgct gcacggcctg acccgtagcc ttgtggccaa
     5341 catggttcag ggcgtcacga gcggctatgc gaagaagctc gagatcgtcg gtgtcggtta
     5401 tcaggcccag ctcaaaaaag ccaacacggt cgcgctgcag gtgggttacg ccaatcaggt
     5461 cgtgctggag gcgccgccgg gcgtgactgt agcggtgcct gatccgaccc atgtcatcat
     5521 tagcggggcc gacaaacagg ctgtcggcca gttcgccgcc gtggtccgca aggttcgccc
     5581 cccagagcca tacaagggca agggaatccg ctacgaaggt gagttcgtcc gccgcaaggc
     5641 gggcaaggcc ttcgggtcga aataatcaat aacagtccgc tcgtcatcag gatcgtaccg
     5701 tgaatatcgc aaaccaacaa aaactcgtgc aaacgcgccg gcagcggcgc cagcaccggg
     5761 tccgcaatcg aatgtccggc acgttggaac ggccccggct cgcggtcttc cgcagctcga
     5821 agcacatcta cgcccagatg gtcaacgatc agacgggtac gacgctggtc tcggccagca
     5881 cgctggaccc cgaggttaag gctcagctca agtacggcgg caacaaggcc gccgcgacct
     5941 tcgtcggccg tgtggtcgcc gaacgagcca aaaaggccgg tattgacaag atttgcttcg
     6001 accgtcgaag ctacaagtac catggtcgcg tggcggcgct ggcggatgcg gcccgcgagg
     6061 cgggcttgaa gttctaatcg aaaagacatt cggccgagga gtctatccgt gtcgagcgag
     6121 ggacgtgaac gcgatcgcga ccgtggggaa tggaccgaaa gcgttgtgtc gattcggcgt
     6181 tgcgctgcgg tggtcaaggg gggccggcgg ttcagcttca atgctctggt cgtcgtcggc
     6241 aatggccgtg gccaggtcgc atggggctac ggcaaggcca acgaagttcc cccggcggtc
     6301 gagaagggcg tcaaggacgc acacaagcaa atgaagcgcg tccagttgcg gggggggacg
     6361 atcccccatg ccgtgattgg ccagtacggt gcggccaagg tgctgttgat gccggcaagt
     6421 cccggaacag gcgtcatcgc cggcggcgcc gttcgggcgg tggtcgagtc ggccggagtc
     6481 aaggacattc tgaccaagag cttcggctcc aataacaagc tcaacctcgt caaggcggcc
     6541 atcgacgcct tgctcaagct gcgaacgaaa gaagagattg ctcgtctgcg aggagtgccg
     6601 ctctaatgca actacacgac gtccaccaag gggttcatcg ccgcaagaaa cgcaaacgga
     6661 tcggccgagg gatcggctcg gggcatggta agacctcgac caaggggcac aaaggccact
     6721 cctcgcgcca gggattcaag ctgagcccga tctttgaagg cgggcagatg cctctggccc
     6781 gccgcgtccc caagcgcgga ttcgttaacg gcgccttcaa aaagagcttc gccatcgtca
     6841 atttggccga cctcgacgcc aagtttgagc cgggggccgt cgtcgatgaa aaggcgctcc
     6901 gctccaaggg gctggtcaag ggccatgcct tcgacggcat caagatcctg ggcgacggcg
     6961 cgctctccaa gaaactcgag gtccacgcga ccaagttcag cggctcggcc gccagcaaga
     7021 tcgccgctag cgggggcaag accgtcgttg tcccctaccg gccccacgct gttcatgctc
     7081 aatcgaagga agaaaaggcc ggcgcggtcg ggtgaatcga ccacgcgggc gaaaagcgcg
     7141 cgatcgctgt cctagttcta aatggatgaa cctggcaccg ggagtagccg gttcgtcgcg
     7201 gtgggcggct gcagactccg cccctttctg atcgctcccg actttcatgc cgcgccgcct
     7261 gtcgcggcga atgcgcgaag gggatcgtcc atggataagc tcttcacgat cttcaaagtt
     7321 ccggaactgc ggcgcaagat cctgttcaca gcgttgctcc tggcgatcta ccggatcggc
     7381 ttttacgtgc cgctgccaat tgtcaaccag gcgcaattga aaaactggga ggagcagcaa
     7441 agcggcaaca ccgcgggcaa actcttcggc actgtggcga tgttcggcgg cacctcgatc
     7501 ggcatgagca cgatcttcgg cctgggaatc atgccctaca tctcggcatc gatcatcttc
     7561 cagcttctgg ccagcgtcgt gccgtcgctg gaagcgatga tgaaggaagg agagagcggc
     7621 cgcaagcgaa tcaacgaata cacgcggtac gcgaccgttg tcttatgcgc catccagagt
     7681 gcgttctggg tgcaatacat gatgagcccg cagatgaacg tggttttgcc cgattaccgc
     7741 gacttctggc acggtatggt cagcgtctgc atcatgacgg ccggcaccat ctttttgatg
     7801 tgggtgggcg aacagatcga cgagtacggc atcggcaacg ggatcagcct tttgatcatg
     7861 gcgggcattt tggcccgttt gccgatcgcg atgaaaggac tctgggagaa ttcaacttcc
     7921 aagttgacgc ccgagccagg caagtacggc atcatcacca tcctgctctt gctcggtctc
     7981 ttcatcgccg tggtggttgg cgtgatcgcg attaccgaaa gccagcggcg cattcctact
     8041 cagtcggcta agcacgtacg gggccgccgc gtcttcggcg gcaccaagca attcctcccg
     8101 ttgaaagtca accaggcggg cgtgatgccg atcatctttg cctcgagcct gctcatcttt
     8161 ccgtcattcg tcttcagcag ccttgcacga gcaacctcgg gcatgcgtac ggacaactgg
     8221 agtgtcgggt cgttcttcgc ggggctcttt caggaggttg ccgacgcgtt tcagcggcag
     8281 agctatattt acacggtgtg ctacatcatc ctgatctatt tcttctgtta tttctggacg
     8341 gcgatcacct ttaaccccaa ggatatggcg gaaaatctca aagactacgg cagcttcata
     8401 ccgggctatc gtcctggtcg ccggaccgcc gattatctcg agaaggtgat gttgcggatc
     8461 acctatgtgg gcgcggcgtt cctggcattg gttgcggtca taccctcact cgtgcaggct
     8521 gctctgagcg ttgactggac ggtggcctca ttcctgggtg gaacggggct tttgatcgtg
     8581 atcagcgtct gtcttgacct ggtgcaaaag attgacagcc acctcgtgat gcgaagctat
     8641 acaggcctcg tgagccggcg atagtggtgg cgccttgcga ctacaaccag ccggagtttt
     8701 gcgtatgata ttggtagtga ccgagtcgtc gcccgagaga gtcttgatcc tggcgtagag
     8761 gagcccgcga cgtgtcttcc caccgaggct tgggatcccg gccccagatg ccggccctgt
     8821 tttcgggtct cgtgcgcaag cccaaggcca agggcggcgc gaaccagacc gaagctcctc
     8881 ccgtgatcaa gctcaagagc ccgcgcgaaa tcgcgctcat gcgcgaggcg gggagggtcg
     8941 tcgccaaggc actcgatcag gttcggcgga tggccgtccc cggagtatcc acggccgaga
     9001 tgaacgaggc tgtcaccgcg attttcgcgg agcacgacgc aattcctttg tttctgggtt
     9061 atcccagctc ggtcaagggc aagccggcgt ttcctgcggt gatttgcgcc agcgtcaatg
     9121 agcaggtcgt gcatgggatc cccaaccggc gcccgctccg cgagggagac atcgtatcgg
     9181 tcgacacagg ttgcaaggtc aacggctggt gtggcgatgc cgcggtgacc ttggccattg
     9241 gtacgatgcg acccgagatc cagaaattgc tggatgtcac ctccgagacc ttgaatctgg
     9301 caatccgcgc catggcacgt tgcggaacct ggctggaagt cgcgagtctg atggagcgtt
     9361 tcgtcaagag ccagggcctt tacgtcgtcg agaagtttgt cggtcatggc atcggccaga
     9421 ccatgcacga ggagccgcag gttccgaact ttgtgagcca atcgctccga aaattcgaca
     9481 tcaagctcga gccaggggtc gtactggcca tcgagccgat ggtcgccttg ggcaccaaag
     9541 acgtccgtac tctcgacgac ggctggaccg tcgagaccaa agatcgacgc tttagcgccc
     9601 acttcgagca taccgtggcg atgacccctg agggcccacg ggtactgacg tatctcgaag
     9661 attgaaacgg ccgttagatc tgtggcgagt tccgcgttcg gaccgcaatc gaatcgccaa
     9721 agtgcaagac gtggcaacgc actcttgcag ttggccgggt tgacgagtat attggctaaa
     9781 ttcggctttg tcctcaagcg tttgcgttcg agaatcatga tgactcccgg tcgtgagctc
     9841 ggggagtcgt tcaaaaccga ttgagaaact atgaaagtgc gatcgagtgt caagcgaatc
     9901 tgtgaaaatt gcaagatcgt gcgccgtcag ggcaaggtct acgtgatttg caccaacccc
     9961 cgccacaagc agcgtcaagg ctaagtggag aggctctctc cgtcagtcgt gcgaatttaa
    10021 tgctgagttg agttgaatcg aagcaaggga gatcgttcgg atgcctcgta ttcttggtgt
    10081 cgatattccc aacgacaagc gcgcggtgat ctcgctgcaa tatatctacg ggatcggccc
    10141 gtacttggcg ggggtgctct gcgagcggac agggatcgac ccggaaaaaa gggcgcgcga
    10201 tctgtctgag gacgacctgg ccaagctggc ttcactcctg gataacgagt atacggtgga
    10261 ggggcagctc cgccgccaag tgcagcaaaa catcgcccgt ttgcgcgaca tcggctgcta
    10321 tcgcggtctg cggcaccgta agggattgcc ggtgcg
//
LOCUS       DATAQL010000369         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5379865, whole genome shotgun sequence.
ACCESSION   DATAQL010000369 DATAQL010000000
VERSION     DATAQL010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5379865"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /locus_tag="VHS97_06415"
     CDS             <1..2081
                     /locus_tag="VHS97_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX3447868.1"
                     /translation="DPKPGDRFEIALIGSTDRLMLPAVLAPDGFAIEQIEDAEGRPVD
                     SVLAPAENSLIVPNLASIVSSSNATLWLEYRAPGRFRLIAEPGSSHPANGELVTDQGV
                     PIIFDPRDWSVVQEAGESRTGLEAFDLALQAARLATHAGFDRLISLPLVRDIELLEHQ
                     IRTAKTVLRRFRGRAMLCDEVGLGKTIEAGLVLSELMIRGLARSVLVLVPPSLIEQWQ
                     GEMRRKFSIELISHDDPTFRERGPGAWNEFDRVIASIHTTKREPHKSSILARRWDIII
                     VDEAHHLRNRNTQAWKFASEIQKQFILLLTATPVQNNLEELFNLVTLLEPGLLNTARQ
                     FQRHFMDKRDKLTPRNVDELHELLAEVMIRNRRSTVGLQFTRRWARTESLALSPPEHS
                     LYQDVTQFVRDHLRAAQARQAEKGKVKGKDGAGLNRMALLSLQMAMGSSSQAAAGTLR
                     KIAEQPKLPASDQIRLTNLAERALAQHESIKIRRLISLLDEFGDKLVIFTQFRATQQQ
                     LERRLKEAGHGIAVFHGGLSRLEKEAAIEQFRGPARLLLCTEAGSEGRNLQFAHAVCN
                     FDLPWNPMKIEQRIGRLSRIGQTRDVYVFNLVAADTVEAAVLHLLEAKLNMFELVIGE
                     VDMILGNLEDEREFPDVVADLWAESADHDDFARRIDDLGDRLLEAKRAYFEQRALDDK
                     LFGSRFAPDQ"
     gene            2113..3225
                     /locus_tag="VHS97_06420"
     CDS             2113..3225
                     /locus_tag="VHS97_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX3447869.1"
                     /translation="MTTHENVMGPSHSPLEEFLRGYAEVSGGLWDEIEPQVYDLMLPG
                     RTAAGEPEIVRLAFDPEAIPEHPGAQLASYGTPLVDRLLADAVNRGRHIVLYMVGLNL
                     APQGLEDRLRRAVTLPSGFELKLEQSRPLHFPQAVFWFEATFVSDQKEQDLLTVAIDV
                     HHSRQVRHLDRLLDRGHLAEKPWTPLAEAPHQGLRTAYPIARDRVVRTLSALANTYHR
                     ELHERLDRQLERISRYYGDLRAEVEEQAQKARNRESDPAKFIARLESLTREEELQGAE
                     LRRKSQLKVNLRLLNLLVIHQPKLLFHTAVATAGTASIVGRTEWVWDPLVESIEAAVC
                     PECQRPTFEFDVTRQGRLVCPNCPSAASSTPRPARR"
     gene            complement(3232..>3500)
                     /locus_tag="VHS97_06425"
     CDS             complement(3232..>3500)
                     /locus_tag="VHS97_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447870.1"
                     /translation="LLLAFFYTVIDVIKFRSWAFFFVVIGVNAITIYVARHFIPFDSI
                     SQHVFGGVARMSGSFGPVIVPLGAVAIEWLLLLHLYRNKIFLRV"
BASE COUNT          696 a         1092 c         1090 g          622 t
ORIGIN      
        1 tggatcccaa accaggtgat cgatttgaaa ttgcgttgat cggatcgacc gatcgtttga
       61 tgctccccgc ggtgttggcg ccggacggat tcgcgatcga acagatcgaa gatgccgagg
      121 gtcgcccggt ggattcggta ctcgctccgg cggaaaattc gcttattgtg cccaatctcg
      181 cctcgatcgt ctcctcctcg aacgccacat tgtggcttga atatcgcgcg cctggccgat
      241 tccgcttgat cgccgaaccg ggatcgtccc atcccgccaa cggcgagttg gttacggacc
      301 agggggttcc tatcattttc gacccgcgtg actggtcggt ggttcaagag gcgggcgagt
      361 cgcggacggg tctcgaggca ttcgacctgg cgctccaggc cgctcggctg gcgacacatg
      421 ccgggttcga caggctcatt tcgctcccct tggtccgcga catcgagctg ctcgagcacc
      481 agatccgcac ggccaagacc gtactgcggc ggttccgcgg ccgggcaatg ctctgcgatg
      541 aagtcggcct gggcaagacc atcgaggctg gactggtgct gtcagagctg atgatccgcg
      601 ggctcgcccg ttcggtcctg gtcttggtgc caccgtccct gatcgaacag tggcaagggg
      661 aaatgcggcg caagttctcg atcgagctga tcagccacga cgatcccacg tttcgtgagc
      721 gcgggccggg cgcctggaac gagttcgacc gcgtgattgc ctcaatccac acgaccaagc
      781 gcgagccgca taagtcgtcg atcctggccc gtcggtggga cataatcatc gtcgatgagg
      841 cacatcacct tcgcaatcgc aatacccagg cctggaagtt tgccagcgaa attcagaagc
      901 aattcatcct cctgttgacc gcgactcccg tgcagaacaa cctcgaggaa ctgttcaacc
      961 tggtcacgct cctggagccc ggactcttga acacagccag gcagttccag cgacacttca
     1021 tggacaagcg cgacaagctg accccgcgca acgtggacga gctgcacgag ctgctcgcgg
     1081 aggtgatgat ccgcaatcgc cggagcacgg tgggcttgca gttcacccgg cgatgggcgc
     1141 ggaccgaaag cctcgcgctc tcgccgccgg agcactctct ctaccaggac gtgactcaat
     1201 tcgtgcgcga tcatcttcgt gcggcccagg ccaggcaggc ggagaagggc aaagtcaagg
     1261 ggaaggacgg cgcgggcttg aatcgcatgg cgctcttgag cctccagatg gcgatgggca
     1321 gttccagcca ggcagcagcc ggaacgctcc gcaaaatcgc cgagcaaccc aaactcccgg
     1381 ccagcgatca gatccggctc acgaacctag ccgagcgtgc gctggcgcaa catgaaagca
     1441 tcaagatccg ccggctgatc agcctgctcg acgagttcgg cgacaagctg gtcatcttta
     1501 cccagttccg agccacccag cagcagctcg agcgccggct caaggaagcc gggcatggca
     1561 tcgcagtgtt ccacggcggg ctgtcgcggc tggagaaaga ggctgccatc gagcagttcc
     1621 gcgggccggc tcgactgctg ctttgtaccg aggccggcag cgaaggccgc aacctccagt
     1681 tcgctcatgc cgtttgcaat ttcgacctgc cctggaaccc tatgaagatc gagcagcgga
     1741 tcggccggct cagtcggatc ggccagaccc gtgacgtcta tgttttcaac ctggtcgccg
     1801 ccgacaccgt cgaggccgcc gtgctccacc tcctggaggc caagctcaat atgttcgagc
     1861 tggtcatcgg cgaggtcgac atgatcctgg gcaacctcga ggacgaacgc gagttccctg
     1921 acgtggtcgc cgacctctgg gccgaatcag cagaccatga cgactttgcc cgccgcattg
     1981 acgacctggg cgaccggctg ctcgaagcca agcgagccta cttcgagcag cgcgctttgg
     2041 atgacaagct tttcggcagc cggtttgcgc ccgaccaatg aggtgtggtg cgtccgagga
     2101 gtatgcaaat cgatgactac tcatgaaaac gtaatggggc cttctcactc gccactcgaa
     2161 gagtttctcc gcggctatgc ggaagtctcc gggggcttgt gggacgagat cgagccgcag
     2221 gtctacgacc tgatgctgcc aggccggacc gccgccgggg agccggagat cgtgcgattg
     2281 gcctttgatc ccgaggcgat ccccgagcat cccggcgccc agctcgccag ctacgggacc
     2341 ccgctggttg atcgcttgtt ggccgacgcc gtcaatcggg gccggcacat cgtgctctac
     2401 atggtcggct tgaacctcgc accgcaaggt ctggaggatc gcctgcgccg cgcggtcacg
     2461 ttgccatcgg gattcgagct caagctcgag caatcgcgcc ccctgcattt tccccaggcc
     2521 gtcttctggt tcgaggcgac gtttgtgagt gatcagaagg agcaagacct gttgactgtg
     2581 gcgatcgacg tgcaccacag ccggcaggta cgccatctgg accgcctgct cgaccgcggg
     2641 cacctggctg agaagccgtg gacgccgttg gccgaggcgc cgcaccaggg gctacgtacg
     2701 gcctacccga tcgcgcgcga ccgggtggtc cggacccttt cggcgttggc gaacacgtat
     2761 caccgcgagt tgcacgagcg actggatcgg cagctcgagc gcatcagccg atattacggc
     2821 gatctgcgcg ccgaggtgga agagcaggcg caaaaagctc ggaatcggga gagcgaccct
     2881 gcaaagttta tcgcgcggct cgagtcgctg acgcgtgagg aagagctaca gggggccgaa
     2941 ttgcggcgaa agagccaatt aaaggtcaac ctgcgtctgc tcaaccttct agtgatccat
     3001 caacccaagc tcctgttcca cacggccgtg gccaccgcag ggacggcctc gattgtcggt
     3061 cggacggagt gggtgtggga tccgctggtc gagtccatcg aggccgccgt ctgccccgaa
     3121 tgccagcgcc cgacgtttga gttcgatgtc acccggcagg gccgactagt ctgcccaaac
     3181 tgcccttcgg cggcaagttc taccccacgc cccgcgcggc gctagtatcg ttcagacacg
     3241 gaggaagatc ttgttgcgat agaggtgcag cagcaaaagc cactcgatcg ccacggcgcc
     3301 caacggcacg atgacgggcc caaacgatcc cgacatccga gcgacgccgc caaagacgtg
     3361 ctgagagatc gagtcaaagg gaatgaaatg gcgggcgacg taaatggtaa tcgcgttgac
     3421 gccgatgaca acaaaaaaga acgcccagga ccggaatttg ataacgtcaa ttacggtgta
     3481 aaagaatgcc aggagcagta
//
LOCUS       DATAQL010000370         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1868546, whole genome shotgun sequence.
ACCESSION   DATAQL010000370 DATAQL010000000
VERSION     DATAQL010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868546"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VHS97_06430"
     CDS             complement(<1..578)
                     /locus_tag="VHS97_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447871.1"
                     /translation="MDTLKLTYRRHQITYKKRRRVPFGLLGLGVLVALAMLGGRADAF
                     MGYGPHTTFGSAGSANGQFTKPLGVAVDDASEDVYVVDEGGKRVEEFSAEGGYLAQFN
                     GSDNPATPSGFEMPTAIAVDNSAGASKADVYVVDSARNTVDKFSSSGAFVFELGSFAS
                     KVIGVAVDNAGHLWVAEENKSVQEFDGSAKNA"
     gene            complement(582..>1776)
                     /locus_tag="VHS97_06435"
     CDS             complement(582..>1776)
                     /locus_tag="VHS97_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447872.1"
                     /translation="YVKGMQVHTPPGLLGMLSNVQLCDAAGAEAGTCGESSKIGTARV
                     ASGAGSHPFEIEGNVYLTGPYGGSPFGLSIVTRAVAGPFNLGLVVVRARIDVNQEDST
                     LTVTTDETGPHAIPQIIFGVPLRLQRITVNIDRSGFMFNPTNCAAKQITATVSGNQQA
                     SAQVSSPFAAGGCKNLSFKPKFTVSTSAHTSRHSGASLDTKLSYPANSIGVQSNIARV
                     KVSLPKQLPSRLTTLQQACTAAQFARNPSLCPSGSIVGIARARTPLLPVGLSGPVYFV
                     SHGGEKFPSLVIVLEGDNVRVDLTGATFISKAGITSTTFNTVPDVPVGTFELYLPEGK
                     YSALAANGTLCKEQSKLKMPTEFVAQNGAVLKQSTKVAVVGCPKAAKGKASKRSPSRH
                     GKGRG"
BASE COUNT          314 a          563 c          534 g          365 t
ORIGIN      
        1 aaagcatttt ttgcacttcc gtcgaactcc tgcacacttt tgttttcttc ggctacccag
       61 agatgaccgg cgttgtctac tgcgacaccg atcactttac tcgcgaagct gccgagctcg
      121 aatacgaagg caccgcttga gctgaacttg tcgaccgtgt tgcgagcgga atcaacgaca
      181 tagacgtcgg ctttggacgc acccgccgaa ttgtccacag cgatggcggt aggcatttcg
      241 aaacctgacg gcgtcgccgg gttgtcggag ccgttgaact gtgccagata gccgccttcg
      301 gctgagaact cctcgacgcg cttgccgcct tcgtctacca cgtatacgtc ttcgctggcg
      361 tcgtcgacgg cgacgcccaa tggtttggtg aactgcccgt ttgcgctgcc agccgagcca
      421 aacgtggtgt gcggcccata gcccatgaag gcatccgccc gacccccgag catcgccaaa
      481 gccaccagca cgcccagccc cagcaatccg aacggcaccc ggcgtcgctt cttataagtg
      541 atctggtgcc tcctataagt gagcttcaga gtgtccatcg atcatcccct ccccttgccg
      601 tgccgacttg gtgacctctt actcgccttg cctttcgcag ccttaggaca accgacaacg
      661 gcgaccttgg tgctttgttt caagacggcg ccgttttggg cgacgaattc ggttggcatc
      721 ttcagcttgc tttgctcctt gcagagcgtg ccgttggcgg ccagcgccga gtacttgccc
      781 tccggtagat acagctcgaa cgtgccgacg ggcacgtcgg gcaccgtatt gaacgtggtg
      841 ctggtgatcc ccgctttgct gatgaaggtg gcgccggtga gatctacgcg cacgttgtct
      901 ccctccagca cgatcacgag cgacgggaac ttttctccgc catgcgagac gaagtagacc
      961 ggcccggata ggccgaccgg tagcagcggt gtccgcgccc gcgcgatgcc cacgatcgag
     1021 ccgctcgggc acagcgaggg gttgcgcgcg aactgtgccg ccgtacatgc ttgctggagc
     1081 gtggtcagcc gcgagggcag ctgcttgggc agcgacactt tcacgcgggc gatattggac
     1141 tgcaccccga tcgagttcgc cggatacgac agcttcgtgt cgaggctggc gccactgtgc
     1201 ctactggtgt gagcgcttgt tgagacggtg aacttgggct tgaacgaaag gttcttacag
     1261 ccgccggcgg caaacgggct cgatacctgg gcgctcgcct gctggttgcc cgacacggtc
     1321 gccgtgatct gcttcgccgc gcagttggtc gggttgaaca taaagcccga ccggtcgatg
     1381 ttgaccgtga ttcgctgcag gcgcagaggc accccgaaga tgatctgcgg gatggcgtgc
     1441 ggccccgttt cgtcggtcgt caccgtcaag gtcgagtctt cctggttgac atcgatcctc
     1501 gccctgacca cgaccagacc caggttgaac gggcccgcca cagcgcgagt gacaatgctc
     1561 aggccaaacg gcgagcctcc atagggaccg gtcaggtaga cgttgccttc gatttcgaac
     1621 ggatgcgagc cggcgccact ggcgacacgg gcggtaccga tcttcgacga ttcgccacag
     1681 gtgccggctt ctgccccagc ggcatcgcac agttgcacgt tcgagagcat tccaagcagc
     1741 cctggcgggg tgtgcacctg catacccttc acgtac
//
LOCUS       DATAQL010000371         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3548810, whole genome shotgun sequence.
ACCESSION   DATAQL010000371 DATAQL010000000
VERSION     DATAQL010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548810"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VHS97_06440"
     CDS             complement(<1..1968)
                     /locus_tag="VHS97_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3447873.1"
                     /translation="MTVRRGVFAGETRRCFIMGLVVAVSSLCRCLTGAVAAEEPASPN
                     APAAAPEARKRMQLPEGFTATVFAAEPDVVQPIAMTIDHKGRLWVVENYSYPIWLGGP
                     QGKDRILIFEDSDNDGRFDRRTVFYDKGTNFTGLELGFGGVWVCATPNLLFISDRDGN
                     DQPDGEPVVKLDGWDTKAQHNMFNGLKWGPDGWLWGCNGIMSRSRVGKPGTPQDERVA
                     IDCGVWRYHPTRAVFEAVAHGTTNPWGLDFDDLGEAFITNCVIPHLFHVVPGAHFQRM
                     YGEDLAPHRYALMETCADHIHWAGGQWTDSRDGKGKHGEAGGGHAHVGAMIYLGDNWP
                     ETYRNSLFTCNLHGKRVNHDRLDRQGSGYVARHEPDFLMANDSWFRGLEMKYGPDGGV
                     YLTDWYDTGECHDTDADNAHRENGRIYKIVHGKTDPVQVDLTRLDDVALARMQLHRNE
                     WYVRTARRLLQERAAAGRDLAPAQGVLREIMGTNTDVTRQLRAAWALYVTGGLDTKAG
                     LALLDHPSEHIRAWGVRLLCDGTTPAPPTLARFAELAKSDPSPKVRLSLASAAPRLPV
                     DARWAVVEPLLSHKEDATDRVLPLMAWYAVEPLVTAARSRAMSVAAVCQIPTVRRFMA
                     RRTVEAHPAAGLAAVLALLKTADNAGCFDLLI"
BASE COUNT          472 a          826 c          735 g          447 t
ORIGIN      
        1 gatcaagaga tcgaagcagc ccgcgttgtc cgccgttttg agcaacgcga gcaccgcggc
       61 cagaccggct gccgggtgtg cctccaccgt gcgccgggcc atgaatcggc ggacggtcgg
      121 aatctggcag acggcagcga cactcatggc ccgcgaccga gcggccgtga cgagcggctc
      181 gaccgcgtac catgccatca agggaagcac tcgatccgtt gcgtcttcct tgtggctcaa
      241 cagcggctcg acaactgccc acctcgcgtc taccggcaaa cggggtgcgg cagaggccaa
      301 gctgagccgg accttggggc tgggatcaga tttggccagc tcggcgaacc gggccaacgt
      361 gggaggggcc ggtgtcgtcc catcgcagag cagcctcact ccccacgccc gaatgtgctc
      421 gctcgggtga tcgagcaatg ccaggccggc tttcgtgtcg aggccgccgg tcacgtagag
      481 cgcccacgcc gcacgcaact ggcgagtgac gtcggtattt gttcccatga tctcgcgcaa
      541 gacgccttgg gcaggcgcca ggtcgcgtcc cgcggccgcg cgttcctgga gtagccgccg
      601 cgccgtgcgg acataccatt cgttgcgatg aagctgcatc cgcgccaggg cgacatcatc
      661 gagacgggtc aggtcgactt gcaccggatc tgtcttgccg tggacgatct tgtagatccg
      721 cccgttctcg cggtgggcgt tgtcggcgtc ggtatcatga cattctccgg tgtcatacca
      781 gtcggtcaga tagacgccgc cgtcgggccc atacttcatt tccaaccccc gaaaccagga
      841 gtcgttggcc atcagaaagt cgggctcgtg acgggcgacg tagcccgacc cttggcgatc
      901 aagccgatcg tgattgacgc gtttgccatg aagattgcac gtgaaaagtg agttgcgata
      961 cgtctcgggc cagttgtcac caaggtaaat catcgcgccc acgtgtgcgt gaccgccgcc
     1021 ggcttcgccg tgcttgccct tgccgtcgcg cgaatcggtc cactgcccgc cggcccagtg
     1081 gatatgatcg gcacacgttt ccatgagcgc gtaacgatgc ggggccaggt cttcgccgta
     1141 catccgctga aaatgggcgc ccggaaccac atggaagagg tgcgggatca cgcagttggt
     1201 gatgaaggct tcgcccaggt cgtcgaagtc gagtccccag ggattggtgg ttccatgggc
     1261 aactgcctcg aacaccgcgc gggtcggatg ataccgccag acgccgcagt cgatcgccac
     1321 tcgctcgtcc tgaggcgtcc caggcttgcc cacccgcgag cgcgacatga tgccattgca
     1381 gccccagagc cagccgtccg ggccccactt caggccatta aacatattgt gctgtgcttt
     1441 ggtatcccag ccgtcgagct tgacaacggg ctccccgtcg ggctgatcat taccgtcgcg
     1501 atcagagatg aaaagaagat tcggcgtggc gcacacccac actccgccaa acccgagctc
     1561 gagaccggtg aagttggttc ctttgtcgta aaagaccgtt cggcgatcga accgtccgtc
     1621 attgtctgag tcttcgaaaa tcaagatccg gtctttgcct tgcggacccc ccagccagat
     1681 cgggtaagag tagttttcga ccacccagag ccgccctttg tggtcgatgg tcatggcgat
     1741 cggctgcacg acatcgggct cggcggcaaa cacggtcgcc gtgaaaccct cgggcagctg
     1801 catccgcttc cgggcctcgg gcgcggctgc aggagcattc ggcgaagccg gctcttccgc
     1861 ggcgactgca ccggtcagac accggcacag actcgacacc gccacgacca accccataat
     1921 gaaacaccgt cgtgtctccc ctgcgaaaac gccgcgacgc accgtcattg tcgactgctc
     1981 ctcaacagaa cgaaaacaat ccgagccggc gtgaatgatt gcttctctcc ggcaattcaa
     2041 tcccgcgggc cgccgattca agaaagcgat tgtaccaaac cccgagactc gagcgcgacg
     2101 ccatgagccc agagaacccg caggaaaacg acgctcacct tgcatcaacc ttcgtttccg
     2161 ccatactctc cctcgtcgtg cccaacaccg cgcgctcatg acgagccggc cgaaatccaa
     2221 ttgcaaaccg ttccgatcga cctggcgtct tgaatcgtga accaaagagt tcgcccgcga
     2281 gtgcccctgc gcccgagcgc tctgtcggcg cgacggaacc atcagaatgt ccactgaacc
     2341 gtctttccgc gccgagcccg aaggtcccgc catcgcggca ggtgattaca ttccccccta
     2401 ccaggtcggc gaccttcctg atccgccgcg ccccggctgg agattgtgga taggcctgat
     2461 cgggcccgga atcgtcctcg
//
LOCUS       DATAQL010000372         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2632204, whole genome shotgun sequence.
ACCESSION   DATAQL010000372 DATAQL010000000
VERSION     DATAQL010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632204"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VHS97_06445"
     CDS             complement(<1..781)
                     /locus_tag="VHS97_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3447874.1"
                     /translation="MAMTDGGGRVPAIRVSGLQYRYPDGNDALRGVTMTVEPGESVAL
                     VGPNGAGKSTLLLHLNGLLPGMVPMAAGHGHHFGWPASNHRRALAPSVWIDGLEVGPA
                     NAALVRRRVGVVFQDPDDQLFCNTVIEDVAFGPLNLGKSATEARQIALDCLARMDLND
                     QAERPPHHLSFGERKRACLAGVLACEPSVLVLDEPSANLDPRGRRRLIQLIERLPCTK
                     LIATHDLELVLELCPRTVLLDQGEVVADGPSREILGNASLIE"
     gene            complement(824..1477)
                     /gene="cbiQ"
                     /locus_tag="VHS97_06450"
     CDS             complement(824..1477)
                     /gene="cbiQ"
                     /locus_tag="VHS97_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX3447875.1"
                     /translation="MRLDGLERQSAGTDILHRLDPRLKLIASLVFVLSVIVTPIGWWT
                     ALGIEGLVLAFVVGFAGIPPRELGRRWLAFFVFFGFLTFMVAPAHPARARYGLGVVAA
                     SILIKNSLALLIMLVLAATTPFHKLLAALRKLGVPPVLVATLQFMDRYRHVLTDELER
                     MSTARRARTFDRRGSLAWSLLTGLVGMLFLRTFERAERVHGAMVARGWTGVIRSLDD"
     gene            complement(1467..2567)
                     /locus_tag="VHS97_06455"
     CDS             complement(1467..2567)
                     /locus_tag="VHS97_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX3447876.1"
                     /translation="MKFLTKRHTAVSMVVSDRSRADLTSWVPAIETAEVTLMHIPDAL
                     LDPRVAAATSVVGATGLLFALRKLERQLGERTTVLMGTMSAFVFAAQMVNFPVGPGVS
                     GHLLGGVLASVLLGPWAGAVVIAAVLLVQCFLFADGGVTALGANFINMGLIGAIGGYL
                     IYAPIRRAIGGQKGVLIGAMTASWFSVILASGAFAIELGASGHWADFFKILSWMALVH
                     SGIGVGEALITGLVVRFLLVRRPDLFEMTDDPTQSPSAGGRWGQTVAGGLAIALAVAV
                     FLSPFAYEKPDGLEFVGEKLGFLKNDSSAHASPIPAPISDYELRLPGLDHVKLATAAA
                     GLVGTLVVFGVACGMARVLPRRQIEEATSHAA"
     gene            2716..3354
                     /gene="pdxH"
                     /locus_tag="VHS97_06460"
     CDS             2716..3354
                     /gene="pdxH"
                     /locus_tag="VHS97_06460"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX3447877.1"
                     /translation="MTISERRKDYTLLNLREDEADPNATRQFVRWLDEAMKAGVSEPD
                     AMTLATATPDGRPSARVVLLRGVDDRGFVFFTNYDSRKGRELAANPWAALVLFWHELE
                     RQVRVEGQVRRVSVQESDHYFQSRPAGSRIGAWASPQSEVIKSRESLEIRCQELEKSF
                     EDGTIPRPPNWGGFRLVPEMIEFWQGRPSRLHDRLRYTRREQSKWLVERLAP"
BASE COUNT          747 a         1124 c         1056 g          570 t
ORIGIN      
        1 cctcgatcag gctggcattt cccaggatct cgcggctggg gccgtccgcg accacctcac
       61 cctggtccag aaggacggtg cgcgggcaaa gctcgaggac cagctcgagg tcatgcgtgg
      121 cgatcagctt ggtgcacggc agccgctcga tgagttggat caggcggcga cggccgcgcg
      181 ggtcgaggtt tgccgacggc tcgtcaagaa ccaggacgga cggttcgcac gccagaacgc
      241 ccgccaggca agcgcgcttg cgctcaccaa agctaagatg atggggcgga cgctcggcct
      301 ggtcgttcaa gtccattcgc gcgaggcaat ccaacgcgat ctgtcgcgcc tcggtcgcgc
      361 ttttccccag gttgagcgga ccaaatgcca cgtcttcgat cactgtgttg cagaaaagct
      421 ggtcgtcggg atcctgaaat accacgccaa cccgacggcg cacaagggcg gcattggctg
      481 ggccgacctc caggccgtca atccagacac tgggtgcaag agcgcgccgg tggttgctcg
      541 ccggccaccc gaaatggtgg ccatgaccgg ccgccatggg gaccataccg ggcaagagtc
      601 cgttcaagtg caaaaggagc gtgctcttgc ccgcgccgtt gggcccgacc agagcgaccg
      661 actcgcccgg ctcgacggtc atcgtgactc cgcgcagcgc gtcgttgccg tcgggatagc
      721 ggtattgcag gccgctaacc cggattgccg gtacgcgtcc gccaccatcg gtcatcgcca
      781 tcgacgttac ttcagcacgc acggattacc aatctttatt tctttaatcg tcgagactac
      841 ggatgacacc ggtccagccc cgcgcgacca tggccccgtg cacccgctcc gcgcgctcga
      901 atgtgcgcag gaagagcatg ccgaccaacc cggtaagcag gctccaggcc agtgagccgc
      961 gccgatcgaa cgtgcgcgcc cgccgggctg tcgacatgcg ctcgagctcg tccgtgagaa
     1021 cgtgccgata gcgatccatg aactgaagcg tggcaaccag gaccggtgga acacccagct
     1081 tgcgcagtgc tgccagcagc ttgtgaaatg gcgtggtagc cgccagcaca agcatgatca
     1141 agagcgcgag actgtttttg atcaggatcg acgccgcgac gaccccaaga ccgtagcgag
     1201 ctcgggcagg gtgcgccggc gcgaccatga aggtgagaaa gccaaaaaag acaaagaatg
     1261 ccaaccagcg ccggcccagt tcccgcggtg ggataccggc aaatccgacg acgaaggcaa
     1321 gcacgaggcc ttcgatgccc agcgcggtcc accagccgat aggggtgacg atcaccgaca
     1381 gcacgaaaac aaggcttgca atcagcttga gcctcggatc gagccggtgc agaatgtcgg
     1441 tacccgcgct ctggcgctcg agcccgtcaa gccgcatgag acgtggcctc ttcgatctgt
     1501 ctgcgaggca aaaccctggc catcccgcag gctacgccaa agaccaccag agtgcccacc
     1561 agtccggcgg ccgccgtcgc cagcttgacg tgatccaggc cgggtagcct gagctcgtag
     1621 tcggaaatcg gcgcgggaat tggcgaagcg tgagcagatg aatcgttctt caagaaacca
     1681 agcttttcgc caacgaattc gagtccgtcg ggcttttcgt aggcaaacgg ggacaggaat
     1741 actgccaccg ccaaggcgat cgcaagcccc cccgccacgg tctggcccca gcgtccgccg
     1801 gccgaggggc tttgggtcgg atcatccgtc atttcaaaga ggtccggccg cctgacgagc
     1861 aggaaccgca cgaccagccc tgtgatcagc gcctcgccca ctccaatacc tgagtgcacc
     1921 agagccatcc agctcagaat cttgaagaag tcggcccaat gcccagaggc ccctagctcg
     1981 attgcgaatg cgccggaggc cagaatcacc gagaaccaag aggcggtcat ggcgccaatc
     2041 agaacgccct tctgtccgcc gatcgctcgc cggatcggtg cataaatcaa atagcccccg
     2101 atcgcgccga taagccccat gttgatgaaa ttggctccca gcgccgtcac gccaccgtcc
     2161 gcgaacaaga agcactgaac gagcaagacg gcagcgatga ccaccgctcc ggcccacgga
     2221 cccagcaaca ccgaggcaag cactcccccc aacagatgac ccgaaactcc cggcccaacc
     2281 ggaaaattga ccatctgggc ggcaaacacg aaggccgaca tcgtccccat cagcacggtc
     2341 gttcgctcac cgagctgccg ctctagtttc cggagcgcga acagcaagcc cgtcgccccg
     2401 accacgctcg tcgccgccgc caccctggga tcgagcaacg cgtctggaat atgcatcagc
     2461 gtaacctcgg ccgtttcaat cgccggcacc cagctcgtca ggtcagcccg ggatcgatca
     2521 gaaaccacca tgctaaccgc ggtatggcgt ttcgtcaaaa acttcatacg agaatcgctt
     2581 ggacaatgtg cgaggttttt ggcacaagcg ttcaggctgt ctacaatgga tgaagaaggc
     2641 cccatcgacg gttccgcgtt tcatgcattt cgtgcattcg cttgagccgg ccgagattcg
     2701 ccgaggattc tgatcgtgac gatttccgaa cgacgcaaag attacactct gttgaatctc
     2761 cgggaggacg aggccgatcc caacgcgact cgccagttcg tgcgctggct cgacgaggcc
     2821 atgaaagccg gcgtctccga gcccgacgcg atgacgttgg caaccgccac gccagatggc
     2881 cggccgtcgg cgcgggttgt cttgctgcgt ggggtggacg accgcggctt tgtcttcttc
     2941 accaattacg acagccgcaa agggcgcgag cttgcggcca atccgtgggc ggcacttgtg
     3001 ttgttctggc acgagcttga acggcaggtg cgcgtcgagg gccaggttcg ccgcgtgagt
     3061 gtgcaggaat ctgaccacta ttttcaaagc cggcctgcgg gttcacgaat cggagcctgg
     3121 gcctcacctc agagcgaagt gatcaagagc agggaatcac tcgaaatccg atgccaggaa
     3181 ttggaaaaaa gtttcgagga tggcacgatc ccccgacccc ccaactgggg cggattccgg
     3241 ctggttccgg agatgatcga attctggcaa ggtcggccca gccggttgca cgaccgcctg
     3301 cgctataccc ggcgcgagca atcgaaatgg cttgtcgaaa ggctcgcacc ttgaagtaag
     3361 ctcttgaacg cgccggccag catgagcaca tccgatcggc caaggaaagg cctcgatccc
     3421 ggcggagctg gccgagagct tcgaggtaga acgccctgag ttggctgacc cgggtacaga
     3481 agttcaaacg gcatcgg
//
LOCUS       DATAQL010000373         7978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2937191, whole genome shotgun sequence.
ACCESSION   DATAQL010000373 DATAQL010000000
VERSION     DATAQL010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2937191"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VHS97_06465"
     CDS             <1..1461
                     /locus_tag="VHS97_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447878.1"
                     /translation="SGSGWHALQTTVPGEYAPTARSFDGPEVPVPPNPAFAEMRGTLD
                     AAQSSMTYAIPIGPMTQSLGVTVRPVAGMPPGDLPVVDELSLVDPTGKTLEQVGSPGS
                     AGGFISAAVLVQLHGARAGDQILVQIGAAEPSSSSPSSPSSTLSSSGSGVATAPASSQ
                     SASWNVPFVLDVQRQQSSASSTDAGSVESGTGTVGTLIVTPAQQSAPFASSSSSSAPA
                     TQDGSTSVDVQSTNTAAAAAAPAAASDSLVTESTDSFNVRVPTGPLASRGGGALGPTL
                     ALIDAEPTQPVDRLERALSQDIVGLGAGDGEQTASRGNGAGDQEFSDDPGTWPGSIGT
                     GGTGGPVVSIPGRGGFPLRVTSPGRDQRGQFQFPALWATLPAAPSPDTAVSNAAQSAI
                     SLNELPIVPAVAYRSAPETLECPDFVKAACGLALGLGLTTGPLFPDLIASFPGRLPKW
                     VSILRAQSRRRTSARDSKRVSHRKMPNWLRALFASR"
     gene            complement(1511..5449)
                     /locus_tag="VHS97_06470"
     CDS             complement(1511..5449)
                     /locus_tag="VHS97_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3447879.1"
                     /translation="MNLSASRRTWEEASSPAAVRLAKKYEQAWRDSELVGRKPELRAF
                     LDEAGAAADQTGARLALLRADMTLRWDAGEKVGARWYLDRYPDLGEDTIVALIYEEFC
                     LREEDQERPDPADYLRQFPEVALALKRVLEIHELIGTGTNPTTLSYSSTNGTGPAGIA
                     FPEVEQTIAGFFLVEELGRGAFARVFLARERQLADRPVALKVTRRGSHEPQTLARLQH
                     THIVPVHSHRVDAATGLHLLCMPYFGRITLSRVLADPEVQTALSGSVLVEALDRLDTA
                     GDLPAGSSAGRLELSGRSYPRAIAWWCARLAEALAHAHDRGVLHRDIKPSNVLVTSDG
                     MPMLLDFNLAREPLADDGTTADSANLGGTIDYMAPEHLKSLGEPLSTGADGRADIYAL
                     GVVLYEALTGRRPFTTPRRGTSVIDALLLATEERLRPLPSIRKRHSEVPPALEAVIRR
                     CLEPAPRDRYQSAAELATDLHAIADDFPLVHAHEPWASSAAGWVRRRRRRLAGAAVIL
                     LAAMAAAVASASVLNDRSKDHRVIRLEYRKGLDAYDNGDFPSAKIHYQAAADLADRYS
                     QNTWGRLFRLKITNIRELGALLTDKVDEIAVPDRVDEIHDHALEKIKLAERNDKTRSD
                     ADALFAAANGLRFRLHLGEGNELISVFEDLQRALEPFFVLKNEDWTKLDPILALLDQD
                     RRQRLLIEVNELLFLWVANIDELLDSRPEPSDQKTEREDSDPVSSALAICNRALVWVE
                     PKDPWLAMAARLRKHQATPANQSLALAAANEGTIEHEPARPSLERSPLACFQCGLLAL
                     RERRLPRAMDWLRRAVQLEPRNHWYQYFLAYLEDTAGYKDEALAHYNVAHVIEPDSPW
                     ILFSRARLYRSKGGFDTALEDMKTALAMLAGRPEAGRVHLERGYLYYELGNFHDARVE
                     YNRVIELDTSGMYAPAARLNLANMDAESGAVGRARQEYDALLADDLRDTSARMSRALL
                     ELRQGQANRAYIDCSTLLDMQENAKDRDQVLATRALALLLLNRPAEAIADAREAQQIR
                     PGPARERLRQRAILAARRIDLLQLEDPDEVALLPLRGRRLTADLRATADALWRLASTR
                     PDQTYRASLTLAVIYAVLDQKDAAEAAATRVLEVSPYAPRGHLIRARIRYYGGDYQGA
                     RNDVEQGLAVQFNEPGLLELKGILRAAAGDYNAALNFFEAAVGCGAVDRIHLHKAAAL
                     MALDQVEKAAQEWSLALRRDPELPEAYLGRARAHLRLRRWDMALADLEQAAAWAHSDP
                     RIELGIVSAYFRCLRHRREYFGRWCALALRTARDIYGVLTLRTSTSTRAH"
     gene            complement(5449..6054)
                     /locus_tag="VHS97_06475"
     CDS             complement(5449..6054)
                     /locus_tag="VHS97_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447880.1"
                     /translation="MSVSEQTQREDGELADFLKRIQSGDEGAARELLERYEAEVRLVV
                     RRQLPRLLRSRFDSLDFLQSVWGSFFRRMRTAPTEFEDSRHLVAFLARAAKNKVIDEY
                     RRSASRKHDMHREEPLWVDGHRPKDLPDPIDSPSEIAQAHEVFGRLRSLVPEEKRSIL
                     EMKAEGLSSKDIGERLGISERTVQRVLEDLRRRMETEWESP"
     gene            complement(6240..7496)
                     /locus_tag="VHS97_06480"
     CDS             complement(6240..7496)
                     /locus_tag="VHS97_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447881.1"
                     /translation="MEGSFACPECGTQVEVRGLAPGRQVRCGFCNRLLEVPYLPRAAE
                     SPWRRRRLGRPKWLPWVWAALAVLFVAILAAAGARFLNRQWHSSQERSINQLVESSRQ
                     HQANGVFGPALIDLDAAIEMACRAGPHLITQLDAWRKERTSLAQRDAQSVIDSLCGAR
                     SAEFPLGNWLNLIARAQRDPDLATFRSSIDHHFQTGLDREVDDDLAAARQGAESGHVV
                     SSFGFCERVAAVLDHLAPGRQSTVRTETRDIVVRLVSTRGVKLEPPRGDFAYGSQTYI
                     AKMLPVLNKALEAKGFLPYREASRWRHEWNHALYQLSLDVSEQLEGSYLSSANRLTRI
                     EARLTLTSLGTSEWHWQTNPTARSTVPLPRLPAYLSSRLAMSRERSDEMERLLYDNAR
                     GQIHDKFVHALNNMPFCPTGQLTSSQ"
     gene            complement(7682..>7978)
                     /locus_tag="VHS97_06485"
     CDS             complement(7682..>7978)
                     /locus_tag="VHS97_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEX3447882.1"
                     /translation="LTFPERFAGVVAINGWLPVGLRPLGRLKACRELPILVVHGAWNA
                     RVPLHDARQNVATLRAGGLRVAFQSYPSAHRLSNPMLSDVDTWLIGHCTADTRP"
BASE COUNT         1457 a         2435 c         2667 g         1419 t
ORIGIN      
        1 tcgggttcgg gttggcacgc cctacaaact accgtgcccg gcgaatacgc gccgacagca
       61 cgttccttcg acggacctga agtgcccgtt cctcccaacc ccgcatttgc ggaaatgcga
      121 ggcactctcg acgcagcaca gtcgtcgatg acctacgcga ttccgatcgg gcctatgact
      181 cagtctctgg gcgtgactgt gcgtcccgtt gcaggcatgc cgcctggaga tctaccggtc
      241 gtcgatgagc tgtcgcttgt cgacccgaca gggaagacgc tcgagcaagt cggatcgccg
      301 ggaagcgccg ggggatttat atctgccgcc gtgctcgtgc agttgcacgg cgcgagggcc
      361 ggtgaccaaa tcctggtgca gatcggggcc gctgagccct cgtcgtcgtc gccctcgtct
      421 ccatcttcga cgttgtccag ttcgggatca ggcgtcgcga cggcgccggc ctccagtcag
      481 tcggcgagct ggaatgttcc atttgtcctg gacgtgcaga ggcaacagtc ctcggcttcg
      541 agcactgacg cgggttcggt ggaatcagga accgggacgg ttggcactct aatcgtcact
      601 ccggcacaac agtcggcacc tttcgcgtca tcctcgtctt cgtcggcacc ggccacgcaa
      661 gatggctcga cctcggtcga cgtgcagtcg accaatacgg ccgcagccgc ggcggccccg
      721 gcggcagcgt ccgatagttt ggtgacggaa tcgaccgaca gcttcaatgt gcgcgtgcca
      781 accggcccgc tcgcatcgcg cggcgggggt gctctcggac caaccctggc cttgattgac
      841 gccgagccga cgcagccggt cgatcgcctc gagcgagccc tctcacagga catcgtgggc
      901 cttggtgccg gtgacggtga gcagacggcc tccagaggca acggtgcggg cgaccaggaa
      961 ttctccgacg acccgggtac ctggccgggt tcgatcggca ccggggggac gggcggaccg
     1021 gtggtctcga taccgggtcg cggcgggttt ccgctcaggg tgacttcacc gggacgcgat
     1081 cagcgcggcc agttccagtt ccccgcgctc tgggcaacgc tgccggcggc gcccagtccc
     1141 gatacggcgg tttctaacgc cgctcaatcg gccatctcgc tcaacgaact gccaatcgtg
     1201 cccgcagtgg cctatcgctc ggcgcccgag acgctggagt gtccggactt cgttaaagcg
     1261 gcatgcggtc tggcccttgg cctgggattg acgaccgggc cgctgttccc cgacttgatc
     1321 gcctcgttcc ctgggcgctt gccgaaatgg gtttcgattt tgagagcgca atccagacgg
     1381 cggacgtccg cgcgcgactc gaaaagagtc tcgcaccgca agatgcccaa ttggctccgc
     1441 gctctcttcg catcgcggtg aacgacgccc accaggcatc gggtcggatg gacaaaccag
     1501 gcccaggcga tcaatgggcg cgggtggagg tgctggtccg aagggtcagc acaccgtaaa
     1561 tatcgcgcgc ggtccgcaat gccagggcgc accagcgtcc gaagtactcg cggcgatgac
     1621 gcaggcaccg gaaataggcc gacacgattc ctagctcgat cctgggatcc gagtgggccc
     1681 aggcggcggc ctgctcgagg tccgccagcg ccatgtccca gcggcgcaac ctcaggtgag
     1741 cacgtgcgcg cccgagatac gcctcgggga gctcggggtc gcgcctcagt gccagcgacc
     1801 attcctgagc ggccttttcc acctggtcga gcgccatcag tgcggccgcc ttgtgcagat
     1861 ggatgcggtc gacagcgcca cagccgacgg ccgcctcgaa gaaattgaga gcggcgttgt
     1921 agtcgccggc ggcggctctt aagattccct taagctcgag cagccccggc tcgttgaact
     1981 ggaccgcaag accctgctcg acatcatttc gggctccctg gtaatcgccg ccgtaatagc
     2041 gaatccgcgc gcgaataagg tgtccccggg gcgcgtaggg agaaacctcg agaacccgcg
     2101 tggcagcagc ttcggccgcg tctttttggt cgaggaccgc gtagatcact gccagggtga
     2161 gcgatgccct gtaggtttgg tcggggcgcg tgctcgccaa tcgccacaac gcgtcggcgg
     2221 tggcgcggag gtcggctgtc aagcgtcgcc cccggagggg aaggagggca acttcgtccg
     2281 ggtcttcgag ctgcaacagg tcgatgcggc gcgcggccag gatcgcgcgt tgccgcagcc
     2341 gctcgcgcgc tggaccaggg cggatttgct gggcttctcg ggcgtcggcg atcgcctcgg
     2401 ctgggcgatt gaggagtaag agtgccagcg cgcgagtcgc caatacctgg tcgcgatctt
     2461 tggcgttttc ctgcatgtcg aggagagtcg agcagtcgat ataagcgcgg ttcgcctggc
     2521 cctggcgcag ttccaagagg gcgcggctca ttcgtgcgga ggtgtcgcgc aggtcgtcgg
     2581 caagcagagc gtcgtattcc tggcgggcgc ggccgaccgc accggactcg gcatccatgt
     2641 tggctagatt caatcgcgcc gcgggtgcgt acattcctga agtgtccagc tcgatcacac
     2701 gattgtactc aacgcgagcg tcgtgaaagt tgccgagttc gtagtagaga taaccccgct
     2761 cgaggtggac cctacccgcc tcgggccgac cggccagcat cgccagagct gttttcatgt
     2821 cttcgagcgc cgtgtcaaac cctcccttgg agcggtagag ccgggcgcgg ctgaaaagga
     2881 tccaaggcga atccggctcg atcacgtggg caacgttgta atgggcaagt gcctcgtcct
     2941 tgtacccagc cgtgtcttcc aggtaagcca aaaagtattg ataccagtgg ttgcgcggct
     3001 cgagctgcac tgctcgacgc agccagtcca ttgcgcgagg aagtcgtctc tcgcgcaggg
     3061 ccaagagacc acactgaaag caggccagag gcgatcgctc tagactcggc cgcgcgggct
     3121 catgctcgat cgtgccctca ttcgccgctg caagcgccag ggactgatta gccggcgtgg
     3181 cctggtgttt tcggagtcgg gcggccatgg ccagccacgg atccttaggc tcaacccata
     3241 ctaaggcgcg attgcagatc gccagcgctg acgagaccgg atccgaatct tcccgctctg
     3301 ttttctggtc agacggctcg ggacgcgaat cgagcagttc atcgatattg gcgacccaaa
     3361 gaaacaagag ctcgttgact tcgatcagta gacgctgacg gcggtcttga tcgaggagcg
     3421 ccaagatggg gtcgagcttg gtccagtctt cattcttgag tacgaagaac ggctcgagcg
     3481 ctcgttgcaa gtcttcaaaa accgagatga gctcattccc ttcgcccaag tgcaggcgga
     3541 atcgcagacc gttggcggca gcgaacaggg cgtcggcgtc gctgcgggtc ttatcgttgc
     3601 gttctgccag tttgattttt tcgagggcgt ggtcatggat ctcgtcgaca cgatcgggga
     3661 cagcgatctc gtcgaccttg tcggtcagga gggcgccgag ctcgcgaatg ttggttatct
     3721 tcaacctgaa gaggcggccc caggtgttct gtgagtagcg gtcggcgaga tcggcagcag
     3781 cctggtagtg gattttcgca ctgggaaaat cgccattgtc gtaagcgtcc aatccctttc
     3841 tgtactcgag ccggatgacc ctgtgatctt tggagcgatc gttcaagacg ctggcactgg
     3901 cgacggctgc ggccatggcg gccagcagaa tgacggcggc gccagcgaga cggcggcggc
     3961 ggcggcggac ccagccggcg gcgctgctgg cccagggctc gtgggcgtgg accaagggaa
     4021 aatcatcggc gatcgcgtgg aggtcggtcg ccagctcggc ggccgactga tagcgatcgc
     4081 gcggcgcggg ctcaaggcag cgccggatga ccgcctcaag cgcggggggg acttcagagt
     4141 gccgtttacg gatcgagggc agaggacgca gcctttcttc ggtggcgagc agtaacgcat
     4201 cgatgaccga agtgccgcgg cgcggggtgg tgaaggggcg cctgccggtc aatgcctcat
     4261 agagcacgac tcccaaagca tagatgtcgg cccggccgtc ggcgccggtt gaaagcggct
     4321 cgcccagtga cttcaggtgc tcgggcgcca tgtagtcgat ggtcccaccc aggttcgcgg
     4381 aatcggcggt ggttccatca tcggccagtg gctcgcgcgc caggttaaaa tcaagaagca
     4441 tgggcatgcc gtcagaggtg accagcacgt ttgatggctt gatatcgcgg tgcaatacgc
     4501 cgcggtcgtg ggcgtgggcg agcgcttcgg ccaggcgggc gcaccaccag gcgatcgccc
     4561 gcgggtacga ccgtcccgac agctcgagcc ggcccgcgga gctaccggcc ggcaaatcgc
     4621 cggcggtatc gagccgatcg agggcctcga ccaggaccga gccggaaagg gcggtttgaa
     4681 cctcggggtc ggcgaggacg cgcgaaagcg tgatccggcc gaagtagggc atacagagca
     4741 gatggagccc agtggccgcg tcgactcgat gcgaatggac aggcacgatg tgggtgtgct
     4801 gcagccgtgc caacgtctgc ggctcgtggg aaccgcgcct ggttaccttg agggcgaccg
     4861 gacggtcggc gagctggcgc tcccgcgcca gaaaaacccg ggcgaaggca ccgcggccca
     4921 gctcttccac aagaaagaag ccggcgatcg tctgttcgac ctcgggaaac gcaatgccag
     4981 cgggcccggt tccgttggtc gaagaatacg aaagcgtcgt cgggtttgtg cccgtaccga
     5041 tgagctcatg gatctcgagc acgcgcttga gggccagggc gacctcaggg aactgccgga
     5101 gataatccgc gggatcggga cgctcctggt cttcctcccg caggcaaaac tcttcgtaga
     5161 tgagcgcgac gatggtatct tcgcccaggt cgggataacg gtcgagatac cagcgagcac
     5221 cgaccttttc gccggcgtcc caccgcaggg tcatgtccgc acgaagcaag gccaggcggg
     5281 cgccggtttg gtctgcggca gcgccagctt catccaggaa cgctcggagc tccggcttgc
     5341 ggccgacgag ctccgaatcg cgccaggcct gttcgtattt tttggccaag cggacggcgg
     5401 cgggcgacga cgcctcttcc cacgtccgcc gactggcgga caggttcatc acgggctctc
     5461 ccactcggtc tccatccgtc gacgcaaatc ctccaagaca cgctggactg ttcgctcact
     5521 gatgccaagc cgttcgccaa tgtccttgct cgagagccct tcggccttca tctccagaat
     5581 cgagcgcttt tcctcgggaa caagcgagcg caggcggccg aatacttcgt gtgcttgagc
     5641 gatctcgctg ggcgagtcga tcgggtcggg taaatccttg ggccgatggc cgtcgaccca
     5701 gagtggctct tcacggtgca tgtcgtgctt gcgactggcg gagcggcgat actcatcaat
     5761 taccttattc ttcgcagccc gcgccagaaa ggcgacaaga tgccgcgaat cctcgaactc
     5821 ggtcggcgcg gtccgcatgc ggcggaagaa gctgccccag acactctgaa ggaaatcgag
     5881 cgagtcgaag cgcgaccgta gcagccgggg caactgacgg cgcaccacca ggcggacctc
     5941 ggcctcgtag cgctctaaga gctcgcgcgc ggctccttcg tcacccgact ggattcgctt
     6001 gaggaaatcc gcgagctcgc cgtcttcgcg ctgtgtctgt tcgctgaccg acatcgcaaa
     6061 gcctcctcgc cgcgaccagg ccgacagccc ggtctcgcac tcgtccttga ccgatctcgc
     6121 ggttcagcgt cccgatcttt cacgtgagac taacccgaac cccgtctcgt tgacaagtga
     6181 aggcaaggtc tccacgcgat cgtggatgtg ctcagaagct tgaacgaaac tcactcatgt
     6241 cactgagagg aagtcaattg acccgtaggg cagaagggca tgttattgag ggcatggaca
     6301 aacttgtcgt ggatctgccc ccgagcgttg tcgtaaagaa gtcgttccat ctcgtcggac
     6361 cgttcccgac tcatggccag gcggctggac aaataggcag gcagtcttgg aagtgggacc
     6421 gtactgcgcg cggtcgggtt tgtctgccaa tgccactcgc tggtcccgag cgaagtgagg
     6481 gttagccggg cctcgattcg cgtcagtcga ttcgccgacg agaggtagct gccttccaat
     6541 tgctcagata cgtccaagct caattgatag agggcgtgat tccattcgtg tcgccaacgc
     6601 gacgcttcgc gatacgggag aaagcccttg gcctcgagag ctttgttcag cacggggagc
     6661 atcttggcaa tataggtctg ggaaccgtag gcgaagtcac ctcgaggggg ctcgagcttg
     6721 acgccgcgcg tggatacgag ccggaccacg atgtcgcgcg tttcggtgcg gaccgtcgat
     6781 tgccggccag gtgcgagatg gtcgagcaca gccgcgaccc gttcacagaa gccgaaagat
     6841 gagacgacat gtccggactc ggcaccttgg cgtgcggccg cgaggtcatc gtcaacttct
     6901 cggtcgagcc cggtttgaaa atggtggtcg attgaggagc ggaaagtggc gaggtcgggg
     6961 tcgcgctggg cgcgggcgat gagattgagc cagttgccga gcgggaactc cgccgaacgg
     7021 gcaccgcaca agctgtcgat cacagactgc gcgtcgcgct gcgcgagtga ggtgcgttcc
     7081 ttacgccagg catcgagctg ggtgataaga tgcgggccgg ctcgacaagc catctcgatt
     7141 gcggcgtcaa gatcgatgag cgccgggccg aaaacaccgt tggcctgatg ctgacgcgac
     7201 gattcgacga gttggttgat cgagcgctcc tgggacgaat gccattggcg attcaggaaa
     7261 cgggcgccgg cggcggcgag gattgcgacg aacaaaaccg ccaaggcagc ccaaacccac
     7321 gggagccact tgggcctgcc caatcggcgg cgccgccagg gagactctgc cgcgcgcggc
     7381 aaatagggca cctcgaggag ccggttgcag aacccgcaac ggacttgacg gccgggcgcg
     7441 aggccgcgca cttccacctg ggtgccgcat tcaggacagg caaacgaccc ctccattcgg
     7501 ctggtctccc cccgacatgg cacgacgcaa gcggccgtgg cgtaatcacg agctcattca
     7561 tccaggataa cctgtggtaa tgagcttttg gagtgcgtca agcactcgat gccgtgaagt
     7621 tcgggaaaac catcggctgg cggcggcgaa gccactcgag tcggggtgag gcgcgggcga
     7681 atcaaggccg ggtgtcggcc gtacagtggc cgatcaacca ggtatcgacg tcagagagca
     7741 tcggattcga gaggcgatgt gcgctgggat aagactggaa ggccacgcgg aggccgccgg
     7801 cgcgcagggt ggcgacgttt tgccgagcat cgtgaagcgg gaccctggcg ttccacgccc
     7861 cgtgcacaac gagaatgggt agttcgcggc aggctttcaa gcggccgagg ggccgcaggc
     7921 cgaccgggag ccagccgttg attgcaacca ctccggcgaa tcgctcgggg aaggtcag
//
LOCUS       DATAQL010000374         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1410403, whole genome shotgun sequence.
ACCESSION   DATAQL010000374 DATAQL010000000
VERSION     DATAQL010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410403"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1251)
                     /locus_tag="VHS97_06490"
     CDS             complement(184..1251)
                     /locus_tag="VHS97_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX3447883.1"
                     /translation="MPLTRPTYQVTELLGDGIGSELSQAVHQLADRLPIRLNFVEVDF
                     RLENRRLRGKQVYDEAVEVISATRVALKHPTTTAEESPNAILRRRLELSVIHRPVYTI
                     PGVPTNFRRELDLDIVRIATGGTYDDPGRMIGEDGAVSLRIVERRPVREAARYAFNLA
                     RKTGKRVTSASKYTIQKATDGLFEQVAVEVAGQFPEVPHFVELFDALLGKIIMVPEKF
                     QIVLVLNEYGDFLSDMACGLAGSLGIGASANLAFDPAAVVRVALFDAAHGTAPDIAGK
                     NLANPTAIFLALSMLLYQIGEITIGQAVKNATLDLLREGVRTRDLGGQESTDSFTEAV
                     AAEVVKRLTQPSPAHPDPTVH"
     gene            1532..2059
                     /locus_tag="VHS97_06495"
     CDS             1532..2059
                     /locus_tag="VHS97_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447884.1"
                     /translation="MSTAGKVLVVLVLLTSVAWIVLASGVAQLNTNGNTRLHDLAEQI
                     EKLQTGFKQTHDEIVSLRDETSSIQEDVDRQIAVLRARQSDVERARSQIQESLSRVQY
                     QLATVKETIEKANTTLKDRIAEQASDQQTLAQAKSEVENLKAKNGELLSQLTTLRNSF
                     ESVYHSNVESLGKTH"
BASE COUNT          498 a          722 c          718 g          467 t
ORIGIN      
        1 cggccgcagc atcaggaaca cgaatcccag gttcatcgcc agcaaagtca tcgtgatcga
       61 cgggatcacg acgatggacc aaacatcctg gaacagggcc agctcacacg cgagaaccaa
      121 gatcaccacc atcaacgcgc ctgtggactt cggcttcgcg aggcgcatag ggatcggctt
      181 tcgtcagtgt acggtcggat cgggatgggc cggtgacggc tgggtcaatc gcttgacgac
      241 ctcggccgcg accgcttcgg tgaagctgtc ggtcgattcc tggcccccta gatcgcgggt
      301 gcgcactcct tcgcgcagca agtcgagcgt ggcgttcttg acggcctggc caatcgtgat
      361 ctcgccgatc tggtaaagca gcatcgacag tgccagaaag atggccgtgg ggttggcgag
      421 gtttttgccg gcgatgtccg gcgcggtccc atgagcggcg tcgaagagtg cgacccgcac
      481 gacggccgcc gggtcgaagg ccagattggc gctggcaccg atccccaacg aaccggccag
      541 tccgcaggcc atatccgaga ggaaatcgcc gtactcgttc aataccagca cgatctgaaa
      601 cttctcgggc accatgatga tcttgcccag gagcgcgtcg aagagctcga caaagtgagg
      661 cacttcggga aactgtcccg caacctcgac ggcgacctgt tcaaacagac cgtcggtggc
      721 cttctgaatc gtgtatttgg aggcgctcgt gacgcgcttg ccggttttac gggcgaggtt
      781 gaatgcatag cgcgccgctt cgcgcaccgg ccggcgctcg acgatgcgca ggctgaccgc
      841 gccatcctcg ccgatcattc tgccgggatc atcgtaggtc ccgccggtgg cgatcctcac
      901 gatgtccaaa tcaagctcgc ggcgaaaatt cgtcggcacg ccgggtatcg tatagaccgg
      961 acggtgaatg acggaaagct cgagccgccg ccgcaagatc gcattagggc tctcctcggc
     1021 ggttgtggtg ggatgtttga gcgcgaccct tgtggcggag atcacctcca ccgcctcgtc
     1081 gtatacttgc ttgccgcgca gccggcgatt ttcgagccga aaatcgacct ccacgaagtt
     1141 gagccggatc ggcaatcggt cggcgagctg atggacggcc tgagagagct cggagccaat
     1201 cccgtcgccc aagagctccg tcacctggta ggtgggacgc gttagcggca aggcaaggtc
     1261 tcctctgcgc cgattgtacg gaccagtccg caaagtcgtc aaacctggcg cgagatgtgt
     1321 cgaactcggc tcgacagctt ggcagcaccc cacaatggga tgtatattaa gttaacttgc
     1381 atcggttcac gctccgcgca gattgccttc tcgatcttcg cgtttcatcg ccacaatccc
     1441 cttggtggga tagcgggaga tgtggttggg ttcgcgcgac cctcgggccg cgctcgtaac
     1501 gaggtttccg actccgggcg ggagaggttc gatgtccacc gcgggaaaag tactggttgt
     1561 cctggttttg ttgacgtccg tggcctggat cgttctggcc tccggagttg ctcagctcaa
     1621 taccaacggc aatacgcggc tccacgatct ggccgaacag atcgaaaagc tccaaaccgg
     1681 ctttaagcag actcacgacg aaatcgtttc cttacgtgat gagacttcgt cgattcaaga
     1741 ggacgtcgat cgccaaatcg ccgtcctccg tgcccgtcag tccgacgtcg aacgggcccg
     1801 ctctcaaatt caggaaagcc tgtcccgggt tcaataccag ctcgccacag tcaaggagac
     1861 catcgaaaaa gcaaacacga ctctcaagga tcgcattgca gaacaagcct ctgaccagca
     1921 gacgctcgca caggcgaagt cggaagtcga aaacctgaaa gccaaaaacg gcgagctttt
     1981 gagccagctc accacacttc gcaatagctt cgagtcggtt taccattcca acgtcgagtc
     2041 gctgggcaag acacactgaa aagtcgtttg cgggattgag cacgctcgtg taggacgcgg
     2101 aatcttcggc aagctctgtc ccggaatcga tgcgattgtc tcgccgcttc cgaacgccac
     2161 tctgtactaa cgctcaccga tcgaggatga acgcccccag ccgtgcctat caaccctagt
     2221 ttgtcacctc aagacgatgg cctaagacga gtggaagcaa gccaacccga tcccggtgat
     2281 ggaggttggg tggggatcgt tgtcaatcgc aactctggca tggggcgcgg cttacgatcg
     2341 gtgcagcggt tagtgcgcgc gttgcgtcgg gctggcttcg tcgagcagct cgcgttcaca
     2401 cccct
//
LOCUS       DATAQL010000375         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3700940, whole genome shotgun sequence.
ACCESSION   DATAQL010000375 DATAQL010000000
VERSION     DATAQL010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3700940"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..2610)
                     /locus_tag="VHS97_06500"
     CDS             complement(148..2610)
                     /locus_tag="VHS97_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3447885.1"
                     /translation="MKLEDHPTVQRIRLKTVPSVPSKTESLDAGWLKQLVLDAGADDV
                     GFVEIGRPALDDQREDILKAFPHAKTLISFVVRMNREAIRTPARSVSNLEFHHSGEEV
                     NDIARSVVQTLEDRGIRAMNPAMGFPMEMAQFPGKVWVVSHKPVAVAAGLGMMGIHRN
                     VIHEKFGNFILLGTILLDAEVSESSQPINYNPCLECKLCVAACPVGAISPDGDFNFSS
                     CYTHNYREFMGGFTDWVEQVADSKHARDYRSRVSDAESASMWQSLSYGANYKAAYCLA
                     VCPAGEDVIGPYLADRKAHLKEVVRPLQEKEETIYVTKNSDAEDHVAKRFPHKKIKHV
                     GNTLRPNSIEVFLKGMPNVFQPGKSTGLNATFHFTFTGREQRQATVVIQKQKISVNEG
                     HVGQPDLHVTADSQTWIGFLRNERNVVWALLRRKIRLDGPLKLLVAFGKCFPSADARH
                     ELVEILPQPSKLRGEPAPYVKNDDATREIRWRGKLTLSDVVDEAHEVKTFRFTPPTGG
                     AIPFEHLPGQFVTLHIAPRGIPTKRSYTIASSPTWRDRIEITVKREDQGLVSRWLHDE
                     LKIGDEVAIEAPNGTFTFSGKEADRVVLIGGGVGITPLMSVVRYLAATNWPGKMHLIL
                     GFRSPRDFIFREELAELQARNANLAVTITMSGPRDEPWSGRVGRIDAALLASTVPDLA
                     AHRVHVCGPPPMMDAVKAALVSLGVPDAQVKTEAFGTVKRDPSAKEAGSTEIAGRAFF
                     LASGVTALVPVDATILDAADTAGVSIDNACRSGTCASCRVRLVSGQVTMAVEDALTDQ
                     DRAEGYILACQAKVQGDIEVDA"
     gene            complement(2652..3107)
                     /locus_tag="VHS97_06505"
     CDS             complement(2652..3107)
                     /locus_tag="VHS97_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX3447886.1"
                     /translation="MGAEQRQVMIDKVASECVAVRLRMLNRVVTNIYDDALRSLDLKV
                     SQMNILVAAVKMGTARPTEVCEHLHLDVSTLSRNVERMKARGWLEVVPDEDGRSQPFR
                     LTPQGRTLLDKAIPAWTEAQQQVRQVLGDGFVEQLNQAIKRVRKLAAAS"
BASE COUNT          592 a          971 c          940 g          673 t
ORIGIN      
        1 ccgttagaac tttttctggc cggcaccagg gctgggaaac agggttgagg cagcgaatcg
       61 gattacaaaa ctaatgaagg taggcggttg cgctgcggcc tcagagccac gggcgaactt
      121 gaacccgaga ctcatgagcg gccctcctca ggcatcgact tcaatatcgc cctgaacctt
      181 agcctgacag gccaggatgt aaccctcggc tctgtcttga tcggtcagcg catcctcgac
      241 ggccattgtc acctggccgg agaccagcct gacgcgacag gatgcgcacg tgccggaacg
      301 gcaggcgttg tcgatggaaa caccggccgt atcggccgcg tcgagtatcg tcgcatcgac
      361 cggaacgagc gcggtcacgc cggaagccag gaagaaagcc ctgcccgcga tctccgttga
      421 tccggcctcc ttcgcggacg gatcgcgttt gaccgtcccg aatgcctctg tcttgacttg
      481 ggcatcgggg acgccgagcg agacgagtgc ggctttgacc gcgtccatca tcggcggcgg
      541 gccgcataca tgcacccgat gggccgcgag atcaggcacg gtcgaagcga gcagggctgc
      601 atcgattcgg ccgactcgcc ccgaccaagg ctcgtctcgc gggccgctca tggtgatggt
      661 gacggcgaga ttggcgttgc gtgcctggag ttcggccagt tcctcgcgaa aaatgaagtc
      721 gcgcggcgac cggaagccga ggatcaagtg catcttgccc ggccagttcg tcgcagcaag
      781 ataacgcacc acgctcatca ggggcgtaat tcccactccg ccgccgatca gtaccacacg
      841 gtccgcctcc ttgccgctga acgtgaaggt tccattcggc gcttcgatcg caacctcgtc
      901 cccgatcttc agttcgtcgt gtaaccaccg cgagaccagt ccctggtctt cgcgcttgac
      961 cgtgatctcg atccggtcgc gccaggtggg tgatgatgcg atcgtgtagg aacgtttggt
     1021 cgggatgccc cgaggcgcga tgtgtagcgt cacgaactgg ccgggtaggt gctcgaaggg
     1081 gattgccccg ccagtaggag gagtgaagcg gaatgtcttc acctcgtgcg cctcgtcgac
     1141 cacgtccgag agcgtgagct tgccccgcca ccggatttct ctagtggcat cgtcgttttt
     1201 cacgtagggc gctggctcgc ccctgagctt ggagggttgc ggtaagatct cgaccagctc
     1261 atgccgggcg tctgcgctcg ggaagcactt tccgaacgcc accagcagct tgagtgggcc
     1321 gtcgagccga atcttccgcc gcagcagggc ccacacgacg tttcgttcgt tcctcagaaa
     1381 gccgatccac gtttgtgagt cagcggtgac gtgcagatcg ggctggccga catgcccctc
     1441 gttcacgctg atcttctgtt tctgaatcac gaccgtcgcc tgccgctgct cacggccggt
     1501 gaaggtgaag tggaacgtgg cgttcaatcc cgttgatttt cccggctgga agacgttcgg
     1561 cattcccttt agaaacacct cgatcgagtt cggccgcagg gtgttgccca cgtgcttgat
     1621 ttttttgtgg ggaaaccgtt tggcgacgtg atcctcggcg tcggaattct tggtgacgta
     1681 aatcgtctct tccttctcct gaaggggccg cacgacctcc ttgagatgtg ccttccggtc
     1741 ggcgagatac ggcccaatga cgtcttctcc ggccgggcag accgccagac agtacgccgc
     1801 tttgtagttc gccccgtagg acaggctctg ccacatcgag gccgactccg catcgctgac
     1861 ccgactgcgg taatcgcggg cgtgcttgct gtcggccacc tgctcgaccc aatcggtgaa
     1921 accgcccatg aactcccggt agttgtgggt gtagcaggac gagaagttaa aatctccgtc
     1981 cggcgagatg gcccccacgg gacatgcggc cacgcacagt ttgcattcca gacacgggtt
     2041 gtagttgatc ggctggctgg actctgaaac ctcggcgtcc aacaagatcg tcccgagcag
     2101 gatgaaattg ccgaacttct catggatcac gttgcggtga atgcccatca ttcccagccc
     2161 tgccgccacc gcgactggct tgtgtgacac gacccagacc ttgccgggaa actgagccat
     2221 ctccatcggg aagcccatcg ccgggttcat ggctcggatt ccccggtctt ccagagtctg
     2281 aaccacgctg cgggcaatgt cgttgacctc ttccccgcta tggtgaaatt cgagattgga
     2341 gactgatcga gccggcgtcc gaatggcttc tcgattcatg cgcaccacga agctgattag
     2401 cgtcttagcg tggggaaacg ccttgaggat gtcttcgcgc tgatcgtcca aagcgggcct
     2461 gccgatctcg acaaacccca cgtcatcagc accagcatca aggaccagtt gtttgagcca
     2521 tccggcatcg agtgactccg tcttcgatgg aaccgaaggt acggtcttga ggcgaatccg
     2581 ctgcacggtc gggtgatctt cgagtttcat ggccaactcc ttattgtata tgcaactatc
     2641 ggtcgaaaaa atcagcttgc ggcggccagt ttgcggactc gtttgatggc ctggttcaac
     2701 tgttcgacaa atccatcccc aagaacctgc ctgacctgct gttgcgcctc tgtccaggcc
     2761 ggaatcgcct tatctaggag cgtgcggccc tgcggtgtca ggcgaaaagg ctgcgagcgg
     2821 ccgtcctcgt cgggcaccac ttccagccag ccacgagcct tcatgcgttc gacgttgcgg
     2881 ctgagcgtcg aaacgtccag atgcagatgt tcgcagactt cagtggggcg agccgttccc
     2941 atcttgacgg cggcgaccag aatgttcatc tggctcacct tcaggtcgag cgaccgcagg
     3001 gcgtcgtcgt agatgttggt gacgacgcga ttcagcatgc gtagccgaac ggcaacgcac
     3061 tcgctggcga ccttgtcaat catcacttgc ctctgctcgg ctcccattct acgcctccac
     3121 actttaattg tatatgcaac tataggcttg tcaagagggg tctcatgatc tttcca
//
LOCUS       DATAQL010000376         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_189126, whole genome shotgun sequence.
ACCESSION   DATAQL010000376 DATAQL010000000
VERSION     DATAQL010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189126"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VHS97_06510"
     CDS             <1..1040
                     /locus_tag="VHS97_06510"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX3447887.1"
                     /translation="DEELTLASPPTLCRLENRIERKTLIRIAGVLVDQFIASHPQPPE
                     HLILDFDATDDPLHGHQEGRFFHGYYDNYCYLPLYVFSGNELVTAYLRPSKIDSSKHT
                     RALLKLLVRRLRQVWSDVKITIRADSGFCRWRLMRWCDSNGIGYVLGLARNKVLERLG
                     ADWIEWAQRDFKKSGQPQRVFGTFGYGAKSWDRPRRVIVKAERTAQGPNPRFIVVNVP
                     GDPQELYEDVYCQRGDMENRIKEQQLDLFADRTSCHRFLANQFRLFLSSAAYVLVQAL
                     RRMALQQTDLEKAQVGTIRLKLLKVAARVVITARRVVFHLASSYPFQDRFRAVFERVM
                     CPPIPRSAGAG"
     gene            1511..2485
                     /locus_tag="VHS97_06515"
     CDS             1511..2485
                     /locus_tag="VHS97_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3447888.1"
                     /translation="MDAKANTLICAAPLSQDDPETVIVPPNLAYLIVVAGEIPGTMVR
                     VNDRGTTLGRSADSSFQISDITVSRQHAFLSVDENGTVYLRDDGSTNGTFVNGKRVTA
                     FRQVSLDDGDRIQLGTSVVLKLARLDPNDELFQREMFERTVRDNLTGLYNRAYLINQI
                     GVLAERNAAQGMGLAVLMLDIDHFKRVNDRYGHLVGDGVLREVASVIRESTRSEDLVA
                     RFGGEEFVVALPISVAHLALDRADRIRTNLAERRIRTEGQEIQVTASIGLAFGPPGRT
                     RNEMALIMTADEALYQAKAQGRNRVVLGHHAMQISPSPTESAEFASFV"
     gene            2920..3789
                     /locus_tag="VHS97_06520"
     CDS             2920..3789
                     /locus_tag="VHS97_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3447889.1"
                     /translation="MCQSFPEAAHQVLPPFSLGVCSWSLQVKSIAELKSFLQQLGTNV
                     VQIACGDPHHASWQEGDRMPAAALESDIVMTGAMLGFPGEDYTTPETIKQTGGFGNPS
                     LCAERIERLKWGIHRTMALGLSDLSLHAGFLPAPGESGRAAVLEALSEAGRLAAAKGI
                     TLAFETGQETADLLRLTLDELGLPNLKVNFDPANMLLYDMGDPIRAVEILGPDIRSVH
                     VKDARRPKVAGQWGAEVPLGEGEVNIPEFVNVLKTNGYSGPLIIEREVGDQAGRLRDL
                     AHGLSYLRECLKM"
     gene            complement(3909..4256)
                     /locus_tag="VHS97_06525"
     CDS             complement(3909..4256)
                     /locus_tag="VHS97_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3447890.1"
                     /translation="MPPTTTKSLSGSRTSKSASAAPARPTAEIRRVAELLKQVSDPTR
                     LQVLILLSEKERNVTELCSDLGTQSQPAVSHHLALLRHGRLIEPRRSGKHNFYALTDA
                     GRQLAHVVNSVVS"
BASE COUNT          915 a         1228 c         1298 g          898 t
ORIGIN      
        1 ccgacgagga acttacactt gcgtcgcctc cgaccctctg ccgtttggag aaccgtatcg
       61 agcgtaagac cctgatccga atcgccggag tcttggttga tcagttcatc gcctctcatc
      121 cccagccgcc ggaacatctg atcctcgact ttgacgccac cgacgacccc ttgcacggcc
      181 accaggaggg ccggtttttc catgggtatt acgacaacta ttgctacttg cctctgtatg
      241 ttttctctgg gaacgagctg gtgaccgcct acctaagacc aagcaaaatc gactccagca
      301 agcacacccg cgccctcctg aagttgctgg tccggcggct tcgtcaggtc tggtctgacg
      361 tgaagatcac gatccgggcc gatagcggct tttgccgttg gagactgatg cggtggtgcg
      421 attccaatgg aatcggctac gtgcttgggc tggctcgaaa caaggtcctg gagcgtctgg
      481 gggccgactg gatcgaatgg gcccaacgcg atttcaaaaa gtcaggccag ccccagcggg
      541 tcttcggcac cttcggctac ggagccaaga gctgggatcg gccacggcga gtgatcgtca
      601 aggccgaacg cacagcccag gggcccaacc cacggttcat cgtggtcaat gtgccgggtg
      661 atccgcagga actttatgaa gacgtctact gtcagcgggg tgatatggag aaccgaatca
      721 aggagcagca attggacctt ttcgcagacc ggaccagttg ccacaggttc cttgccaacc
      781 agtttcgtct tttcttgagt tccgcggctt atgtcctggt tcaggcattg cggcgaatgg
      841 cgttgcaaca gacagacctt gagaaagccc aagtcgggac gattcggctc aaactcctga
      901 aagtggccgc aagggtggtc atcactgctc ggcgagtcgt gttccacctg gcgagtagct
      961 accccttcca ggatcggttc cgggcggttt tcgagcgggt aatgtgcccg ccaatcccgc
     1021 ggagcgcggg agcaggttga agacggcaaa aaaaccgggc cacagacaac cccaaatgaa
     1081 tgggggtcag ggggaaggtt tgcgcacgcg acagaaaatg gctgccgtcc ttcaagaata
     1141 cagaacaatt cgcttatggg tcccacgccg ggccgggctc gaacctcgga tgaaatattc
     1201 gggctagacc aatattgagc acgatgcgca tgcccgagag accccacgga agatgttatt
     1261 gagcccctcg ccgttgttcg ttggcgacaa tttggtgggt tcttaaccgg ttcatttgac
     1321 acgtgaaatc ggtgacctag gctaacgttg cagacggctc tgacttggaa accgttcgcc
     1381 agagtccggt gcaatgactc cagggtgcga tccattctcg cggcgggaaa aagggccgcc
     1441 tggaatcgac ccacgttcat acgagatcac cttagagccg accgcccggc ttgtcttatc
     1501 gaggtcgatc atggatgcaa aagccaatac cctcatatgt gctgcacccc tgtcgcaaga
     1561 cgatccagag acagtgattg tgccaccaaa tttggcgtac ctgatcgtcg tcgcgggcga
     1621 aatacccggc accatggtgc gcgtgaacga ccggggcacg acgctggggc gatcggctga
     1681 cagttcgttt cagatcagcg atatcacggt atcgcggcaa cacgcgttct tgtcggtcga
     1741 cgaaaacgga acggtctatc tccgcgacga tggcagcacc aatggtacgt tcgttaacgg
     1801 caaacgagtt accgcgttcc gtcaggtcag tctcgacgat ggtgatcgca ttcaactagg
     1861 tacgagcgtc gttctcaaac ttgctcgact cgacccgaat gacgagctct ttcagcggga
     1921 gatgttcgag cgcacggtac gcgataactt gaccggcctg tacaaccgtg cttatttgat
     1981 caatcagatt ggagtcctgg cggagcgcaa tgcagcccag ggaatggggc tcgcggtctt
     2041 gatgctggac atcgaccact tcaaacgcgt caacgaccgt tatggccatc tcgtcggtga
     2101 cggagtcttg cgtgaagtgg cctccgtcat tcgtgaatca accaggtccg aggatctggt
     2161 cgcccgcttt ggcggggagg agtttgttgt ggcattgccg atctcggtcg ctcacctggc
     2221 gctcgatcgc gccgaccgga ttcgcaccaa tctggccgag cgcaggatca ggactgaggg
     2281 ccaagaaatc caggtgacgg caagtatcgg actggccttt ggaccgcccg gtcgtacgcg
     2341 caatgagatg gcgctgatca tgacggcgga tgaggctttg taccaagcca aggctcaagg
     2401 ccgcaatcgc gttgttttgg gtcatcacgc catgcaaatc tcaccaagtc cgaccgagtc
     2461 ggccgaattc gcgtcgtttg tctgacaaag acggctgcgt cccgcctctg cctttgccaa
     2521 cggcgcgttt gtgccgctgg gcgcgccagc agtttctgcc atcatagtat cgtccattca
     2581 gggcaatgcg catggttttc gcgttgaaaa cccagggcgt tgccctgggc tgatctgtga
     2641 atgccccttt ggggcaaatc gccaaaggca ccgcttccgg gatcgccccg aaggggctgt
     2701 cacatgcaag cccagggcaa cgccctgggg attccaaaca catcaaaccc tgccctgtag
     2761 gggccgaaca tcggcaattg tccgcgtttg ccagaatccg cgggcacacc ctttgccgct
     2821 tcctcgaaaa gacgacttcc gccggatatg atcgagtggc gcttgtccgg cgagcgccgg
     2881 accaatagcg atcgatccca cgagcgagag ggagcaacca tgtgtcaatc ttttccggaa
     2941 gcggctcatc aagtgttgcc tccattctcg ttgggggtct gtagctggag ccttcaggtc
     3001 aagagcatcg ccgagctcaa gagcttcctg cagcaattag gaacgaacgt tgttcagatc
     3061 gcctgcggtg acccgcacca cgcgagctgg caagaagggg atcgaatgcc tgccgcggcg
     3121 ctcgaatcgg acattgtcat gaccggtgct atgctgggct ttcccggaga agattacacg
     3181 acgccagaga cgatcaaaca gacagggggc tttgggaatc cctcactgtg cgccgagcgt
     3241 atcgagcgtc tgaaatgggg cattcatcgg acgatggcgc tgggcctctc cgatctctcg
     3301 ctgcacgccg gctttttgcc cgcgccaggc gagagtggca gggctgcggt tctcgaagct
     3361 ttgagcgagg cgggtcggct ggccgcggct aaggggatca cgctggcatt cgagaccggc
     3421 caggagacgg cagaccttct tcgacttacg ctcgacgagc tgggtttgcc aaacctcaaa
     3481 gttaactttg atcccgccaa catgttgctc tacgacatgg gcgacccaat tcgagccgtc
     3541 gagattctcg gccctgatat tcgatcggtc cacgtcaagg acgcccgccg tcccaaagtc
     3601 gcgggccagt ggggcgcgga ggttccgctg ggagagggtg aagtaaatat ccctgagttt
     3661 gtgaatgtgc tcaagacaaa cggatactcg ggaccgctca tcatcgagcg ggaagtcggc
     3721 gatcaggcag gccgcttacg cgatctagcg catggcctct catatctccg cgaatgcttg
     3781 aagatgtgaa aatcaagaag ggccgacctc tgggtggttg gtcttcaccc aacagtcgac
     3841 ccgaagcgct cgatcatgct cggcaattca cgcagcgcga gatttaagtg tggggaaccg
     3901 gcgtcgactt aactcacgac cgaattaacg acgtgggcta actggcgacc agcgtctgtc
     3961 agggcgtaaa aattgtgctt gccggatctt cggggctcga tcaagcggcc gtgacgcaat
     4021 agcgcaagat ggtggctgac ggcaggctgg ctctgggtcc caagatcgct gcaaagctcg
     4081 gtcacgttgc gctctttttc actcaaaaga atcagcactt gcaaccgggt tgggtcggag
     4141 acctgcttga gtagttccgc gacgcggcgg atctcagccg tcggtctcgc aggcgccgcg
     4201 cttgcagact tagaggtgcg cgaacccgaa agcgatttcg tggtggtggg aggcatcgtc
     4261 ttccccaaag ataaatcctg tttgagtttc ggtcaggaga gcgcccgctc ggaagcaaaa
     4321 tctacgtagt taaggatca
//
LOCUS       DATAQL010000377         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4005650, whole genome shotgun sequence.
ACCESSION   DATAQL010000377 DATAQL010000000
VERSION     DATAQL010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4005650"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..618)
                     /locus_tag="VHS97_06530"
     CDS             complement(151..618)
                     /locus_tag="VHS97_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447891.1"
                     /translation="MIAVTGAAGAYGGYVVQFSKADGLTVIADASDKRWSNPWVPICR
                     GDDFTQRIRQHFPNGVDGLADGAVLKEKVIPAVKDGAAFTSVRGFEGVRTHSIRFSAT
                     YVWDYTFEHDKLDRLRQFVDEGKITLRVAAPYKPEGHSRLEARGTRGRIVITF"
BASE COUNT          298 a          495 c          473 g          282 t
ORIGIN      
        1 aaaaccgaac agtcgtcacg ccacgacgtc ggcacgggat atctaagaag ccgccgtggc
       61 atgtgtccgt gagcacgtca gcgttttcct ttgggcgcgg gacgtgtccg agccggacgg
      121 tcacgcggcg catccaaatc gattcgtcgc ctagaacgtg atcacgatcc tcccccgcgt
      181 gccgcgtgcc tcgaggcggc tatgcccttc gggcttgtag ggcgcggcga cgcgcagggt
      241 gatcttgccc tcgtccacga actgccgtag tcggtcgagc ttatcgtgct caaaggtgta
      301 gtcccacaca tacgtggcgc tgaagcggat gctgtgcgtc cgcacgccct cgaagccgcg
      361 caccgaggtg aaggcagcgc catccttgac cgccgggatc accttctcct ttagcaccgc
      421 gccgtcggcg aggccgtcga cgccgttcgg gaaatgctgg cggatgcgct gggtgaagtc
      481 gtcgccacgg catatcggca cccagggatt tgaccagcgc ttgtccgaag catcggcaat
      541 caccgtcagc ccatccgcct tggagaactg cacgacatag ccgccatagg cgccggccgc
      601 gccagtgacg gcgatcacct atccgggctt gagccccaac agttcgagtg agcgccgcgc
      661 ggtgaggccg ttcatcggca aggtcgccgc ctccgcatgc gacttgccgg catgtgcgcg
      721 cacaacggaa cgcgcatcga gcacaagctg ctcgcgatac gctctggctc gctttcggaa
      781 acatgatcgc tagcgccgca tcgcccggct tgatgccggt cgcaacgccg ggcccgacct
      841 cgtcgacaat gcccgccgct tccgtgcccg gtacataaaa tggcggaccg gctttcatct
      901 gctcggcacg cgcgccgccg cacaaaagcg tgtcggtcgg gttgaccgtc gccgcctggt
      961 tgccgatgcg cacctggccc gcgcccgcgt gcacttcggg cagatcgatg acctgaagca
     1021 cttcgggtcc gccaagatcg agacaacgaa gtgccattgc ttccgcccgc tagcgtggga
     1081 catcaatggc tttcgtccag tcattgaaga tgagcgcgct actgacatca gtgcgttcaa
     1141 aaagattctc gatgtgacgg ggactgacaa gcgagttgcg gaagatgcct gaccatgacc
     1201 gtctttcatc accacgatga attgaattca ccacaaatgc tctttcagcg agagcatact
     1261 gatgcccccg tatgttcggg cgacaggccc ctgtccgagc gggcatacgg caactccggg
     1321 gagcctgacg gcgaagcagg ctccccaccc tttccgcgct agcagaaccg acgtgcaatg
     1381 cgggaacgtg aatgacgagg tgtgaccatg cggttcacga ttgaggaggt taaaaagcga
     1441 gcgagcgatc ttggtccgaa gtttcggcgg tggcgcgcaa cccacgtcat gccggacggc
     1501 ggctcgatcc aactgcacta ttgggctatt aatctggctg gagccaaa
//
LOCUS       DATAQL010000378         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_189166, whole genome shotgun sequence.
ACCESSION   DATAQL010000378 DATAQL010000000
VERSION     DATAQL010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189166"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2944
                     /locus_tag="VHS97_06535"
     CDS             <1..2944
                     /locus_tag="VHS97_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447892.1"
                     /translation="YRDAGHYYYPLNKRVQEEWNQSRWPLWEPEENAGMPLLGNPTAA
                     VLYPGKVVFALLPYAWGARVYIVAHSALAFLAMLVLMRSWGTSWIGSALSGLGYAFGA
                     PILFQYCNIIYLIGAAWLPLGVHAVDRWARLGRRWGLLELTVVLSMQVLGGDPQAAYL
                     LGLASIGYALGLAWHRARLSHEAAGGPKPDGSPVLVWLALLAAVGLVVWCAVTLAFAQ
                     WLPGLREPGRPVPPFRWMLWVPVGAAVTWGTVAICVFVYWFRRGWWLKRGWRFPLGLT
                     WLGIGGAAALAATVTAVQLLPVLEFTGLTSRAAAGGPHVIYPFSIEPYRLLELVWPNI
                     LGTQFDGNKYWGSLFKWPGVYPKVWVPSLYLGGLTFVLAMSSLTIRRGPPWRVWFSVV
                     ALVSLVASLGQYTSPIWISRVAANMSGSEWLRKSLADVGPVDRYDSTPIREDGCLRDG
                     DGSFYWLVTTIMPGFRQFRYPAKLFTLTALGMAALAGFGWDRLCEQQARGVRVIFMVL
                     FLVTSAVLTWVVAERQNILKSFSAIPPSSTFGPIQPIGTYEEVLRSLAQAAIVFGAGV
                     ALTVVARKRPWLAGAAALILMTADLALANARYIVTVPQSVFDTKPEILAKIEAAERDQ
                     PASGPYRIHRMPSWSPPGWNNTPSKDRLFELISWERDTIQPKYGIDYGVEYTHTMGVA
                     ELYDYDWYFSGFPRKVHNVETARQLGIETEREVVYFPRRGFDMWNTRYFITPAVPNGW
                     RDETRGLAAFLFNSDLIYPDRAAFPCPEEIENFRNWAETHDYQIFRNRLHLARAWVVH
                     DARTTVPVTGLSRESRSHAMQEILYAADEIWNDRTQVAYDPRKIAWVANDDSPALRQF
                     LSGHATRPSESVKVTYPNPQQAVLEVNLESPGIVVLADVFYPGWKLTIDGKPAPIYRV
                     NGSMRGAAVPAKSHRLIFTYDPQSFRIGRLVSVAGLALLVCLGLACARWPIDPVLGWA
                     DQALSFRH"
BASE COUNT          517 a          898 c          966 g          691 t
ORIGIN      
        1 ttatcgggat gccggccatt attactatcc gctcaacaag cgagtacagg aggagtggaa
       61 ccagagtcga tggccgctgt gggagcccga ggagaatgca ggcatgcctc tcctgggaaa
      121 tccgacggcg gcggtacttt atccgggcaa agtggttttt gcactcttgc catatgcctg
      181 gggggcccgg gtttacatcg tggctcattc ggcgctggcg tttctcgcga tgctggtgtt
      241 gatgcgctcg tggggtacaa gctggattgg atcggcgttg agtggcctgg gttacgcctt
      301 cggcgctccg attttatttc aatactgcaa cataatttac ttaatcggcg cggcctggct
      361 gcccctgggc gtccacgcgg tcgatcgctg ggcccggctg ggccggcgct ggggcctact
      421 ggaactgacg gtagttttgt caatgcaggt gctgggcgga gacccgcagg cagcgtatct
      481 gctggggctt gcgagtattg gctatgccct cgggctggcc tggcatcgag caagactgtc
      541 ccatgaagcg gctggcgggc ccaagcccga tggctcgcca gtcttggtgt ggctggcgct
      601 gctggccgcg gtcggtttgg tagtctggtg cgcggtgacg ttggcgtttg ctcagtggct
      661 gcccggattg cgagagcccg gcagaccggt tcccccattt cgctggatgc tctgggtacc
      721 cgtgggtgcg gcagtgacct ggggtactgt tgcgatctgt gttttcgttt actggttcag
      781 acgcggctgg tggctcaagc gcggctggcg cttcccgctg ggactgacat ggctcggcat
      841 aggcggcgcg gcggcgcttg cggccactgt gacagcggta cagctcttgc cggtgctcga
      901 attcactggg ctgacctcgc gcgctgccgc tggtggcccg catgtgattt atcccttcag
      961 tatcgagcca taccgcctgc tcgagcttgt ttggcccaac attctcggaa cgcaattcga
     1021 tggcaataag tactggggca gtctgttcaa atggcccggg gtatatccca aggtttgggt
     1081 gccgtcgctt tacctcggag gattgacctt tgtgcttgcc atgagcagcc tgacgattcg
     1141 acgcgggcct ccttggcggg tctggtttag tgtggtcgcc ttggtgagcc tggtggcgag
     1201 cctgggtcag tacacgagcc ccatctggat ctctcgggtg gccgccaaca tgtcgggatc
     1261 ggaatggcta cggaaatcgc tcgcggatgt tggccccgtc gaccggtacg atagcactcc
     1321 gattcgcgaa gacggatgtc ttcgcgatgg cgacggcagt ttctactggt tggtgacgac
     1381 gatcatgccg ggctttcgcc agttccggta cccggccaag ttgttcacgc tgacggcact
     1441 cggcatggcc gcacttgcgg gcttcggctg ggatcggctc tgcgaacaac aagcccgcgg
     1501 tgtcagggtg atatttatgg ttctgttcct cgtgacgtca gccgtcctta cgtgggtcgt
     1561 cgcggagcga cagaacattc tcaagtcatt tagcgcgatc ccgccgtcct cgacgtttgg
     1621 gccgattcag cccatcggga cctatgaaga ggtcctgcgc agtctggcgc aagccgcgat
     1681 cgtctttggg gcaggcgtcg cattgacagt tgttgcccgc aaacgcccct ggttggccgg
     1741 cgctgctgcc ctgatcctga tgacggccga cctggcattg gccaacgcac gctacatcgt
     1801 gacggtgccg cagtcggtgt ttgataccaa accggaaatt ctggcgaaaa tcgaggccgc
     1861 cgagcgcgat caacctgcca gcggccccta ccggatccat cgcatgccct catggtctcc
     1921 gccgggttgg aacaacactc catccaagga tcgtctgttc gagctgatct catgggagcg
     1981 cgacacaatc cagcccaagt acggcatcga ctacggcgtc gaatacaccc atacgatggg
     2041 cgtcgccgaa ctctatgact acgactggta tttcagtggt tttccgcgga aggttcacaa
     2101 tgtcgaaacc gcccggcagc tgggaatcga aaccgagcgg gaggtcgtct attttcctcg
     2161 gcgcggattc gacatgtgga atacacggta tttcatcacc cccgccgtgc ccaacgggtg
     2221 gcgcgacgag actcgcgggt tggcggcgtt tcttttcaac agcgacctta tttatcccga
     2281 tcgcgccgcg tttccttgtc cagaagagat cgagaatttc aggaattggg cggaaaccca
     2341 cgactatcag atatttcgca accgtctgca tctagcgcgg gcgtgggtcg ttcacgacgc
     2401 ccgtacgaca gtgcccgtca ccggactttc ccgtgaaagt cgcagccacg ctatgcaaga
     2461 aatcctgtac gccgctgacg agatctggaa cgacaggact caggtcgctt acgatccgcg
     2521 gaagattgcc tgggtggcca acgacgactc gcccgctttg cgccagtttt tgtccggtca
     2581 cgccaccagg ccgtccgaga gcgtcaaggt cacctatccc aatccgcaac aggcggtact
     2641 cgaggtcaat ctcgagtccc cgggtatcgt cgtcctcgct gacgtctttt acccgggttg
     2701 gaagctcaca atcgacggca agccagcgcc catttaccgg gtcaacggct cgatgcgcgg
     2761 agcggcggtc ccggcaaagt ctcatcggct aatctttacg tatgatcccc aatcattccg
     2821 catcggccgt ctcgtctcgg tcgctggcct agcattactc gtgtgcttgg ggttggcgtg
     2881 cgcccgttgg ccgatcgatc cggtattggg gtgggctgat caagcgttgt ccttccggca
     2941 ctgaaacgcc ccctccattt tcattgtgca actccggcga gtgcagcgac caagtttcgg
     3001 catcggggcg gtcgactcag ggtcactgcg tgcgtcgtgg ggcaaccggg gcggacgccg
     3061 tccgaccacg at
//
LOCUS       DATAQL010000379         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3549140, whole genome shotgun sequence.
ACCESSION   DATAQL010000379 DATAQL010000000
VERSION     DATAQL010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549140"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VHS97_06540"
     CDS             complement(<1..206)
                     /locus_tag="VHS97_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3447893.1"
                     /translation="MELGLYTFGDLPVGARSPEAARRRLAEIVAAAKLADQAGLSVFG
                     IGEHHRPDYAISAPEVVLGTIAGTT"
     gene            complement(690..>1515)
                     /locus_tag="VHS97_06545"
     CDS             complement(690..>1515)
                     /locus_tag="VHS97_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3447894.1"
                     /translation="GQASKLVSKLETDLGVQLLKRTTRALSPTEVGQAYYERMKALLE
                     EFDVLDASVRNASSTPSGRVRLTLPISFGAIWLAPALLDFARAFPDIQLDVNFSDRVV
                     NLVDEGFDVAVRIGNPTDSTLIARKLCDMRIVVVGAPAYLEKSGEPRRPEDLVGHDCI
                     IDTNFRDPFNWHFGTANPGESVIVKVSGRLRFSNGEACLAATQAGLGITRIPSFIAGP
                     SIRDGQVRPLLGDAEAAPFRVYAVYPSGKHLAVKVRALVDFLANCFRGKPEWDRGW"
BASE COUNT          297 a          461 c          479 g          278 t
ORIGIN      
        1 gttgtcccgg caatcgtgcc gagaacgact tcgggcgcgg agatcgcata gtcgggacgg
       61 tgatgctcgc cgatgccgaa gacggaaagt ccggcttggt cggcgagctt cgcggcggcg
      121 acgatctccg caaggcgtcg acgggcggcc tcggggctgc gcgcccctac aggcaggtca
      181 ccgaaggtgt agagtccaag ttccatctca cgcctctttg cccgcggccg cgccgcggcc
      241 tcaagtgatt tctccccgga tcggcgcgat caaaagagcg ccacgtaccc cggcattcat
      301 gccgggggct cagagcgcag ccggcgctag ccggctcctc tagcggtgta tccatatcca
      361 tccgcgcgtt gctgaaggcg gcactgccac ccactcaaca ctccagcccc tgcccttagg
      421 gatgcggttt accagactaa gccaacggtg cgcggttacg ccgctcgcgc cggcgctcag
      481 ctttgtcgag ggcagatgca ccttcgggtg cgcatcgaaa ggcccgtaag ggcaaactaa
      541 aagcatctca cttcagggag gggctggctt acaatgctat ggacatgatc gcattccttc
      601 cgtggatttg ttccgaaaaa gcgtgcattc actccattgc gaatacaatc tattgaaggg
      661 gaactattca tcttccgtat tggaagggat caccagccac gatcccactc gggttttccc
      721 cggaagcagt tggcaaggaa gtcaacgagc gcgcgcacct tgacggcgag gtgcttgccg
      781 gacggataga ctgcatagac acggaaaggc gcggcttcgg catcgccgag cagcggccgc
      841 acctgcccat ccctgatgct gggaccagcg atgaagctcg gtatccgggt gatgccgagg
      901 ccggcttgcg tcgccgcaag acacgcctcg ccgttcgaaa agcgcaggcg tcccgatacc
      961 ttgacgatga cagactcgcc ggggttcgcg gtcccgaaat gccaattgaa ggggtcgcgg
     1021 aaattggtgt cgattatgca gtcatgaccg accagatctt cgggacgtcg cggctcgccg
     1081 ctcttttcga gataggcagg cgcgcccact acgacgatcc gcatatcgca aagtttgcgc
     1141 gcaatcaggg tgctgtccgt cgggttgccg attctgacgg cgacgtcgaa gccttcatcg
     1201 acaaggttca caacgcggtc agagaagttc acgtcgagct ggatatcggg aaaggcgcgc
     1261 gcgaagtcca gaagtgcggg tgcgagccag atcgcgccga acgatatcgg caatgtcagc
     1321 cgcacccgtc ccgagggagt gctggaggcg ttgcggacgg aggcgtccag gacgtcgaat
     1381 tcctccagca gcgccttcat ccgctcgtag taggcttggc cgacctcggt cggagacagc
     1441 gcccgtgtag tccgtttgag aagctggacg ccgaggtcgg tctccagctt cgagaccagt
     1501 ttggacgcct gaccg
//
LOCUS       DATAQL010000380         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3244064, whole genome shotgun sequence.
ACCESSION   DATAQL010000380 DATAQL010000000
VERSION     DATAQL010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244064"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VHS97_06550"
     CDS             <1..440
                     /locus_tag="VHS97_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447895.1"
                     /translation="LQCLGAAIRAGGGAVVKTVGEGVVASFADVTSAVKTALDLLPQL
                     ARGAATRPLRLRVAIHRGTTVAATLNDQLDYFGTAARQTIQILDDARGDELVLTQAVA
                     ADPEVAGLLATRQIEGEVVPSRLAGHNHLIRVRLDAQVAGEKS"
     gene            691..4080
                     /locus_tag="VHS97_06555"
     CDS             691..4080
                     /locus_tag="VHS97_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3447896.1"
                     /translation="MKNLVPFNKAAAWAWVFAVALAADVPGFADDEGKTTPSGSQSQA
                     YNPPVAPASDEGLRAMRSFRVPAELNVELFAAEPHLANPVSFCIDEKGVFYVAETFRH
                     GAGVTDTRGHMNWLDDDLACRSVADRVAMYKKFLGKEFAGYNLQHERVRRIVDRDGDG
                     KADAATIFADGFNDPAAGIGAGVLARKDNVWFTCIPWLWKLRDTNGDGRADDRILLHE
                     GYGIHVGFLGHDLHGLRFGPDGRLYFSIGDRGFNVTTREGQRLTVPDTGSVLRCNPDG
                     TELEVFATGLRNPQELAFDEYGNLFTGDNNSDSGDRARWVYVVEGGDSGWRIGYQFIE
                     APISRGPWNEERLWYPPFAGQAAYIIPPIANIADGPSGLTYDPGVTLLPAQFKNHFFL
                     VDFRGSSGQSGIRTFSLQPKGASFQLVNPRQFVWSVLATDADFGPDGALYFCDWVEGW
                     EKTNKGRIYRVMDRARRDLPQVLEVKTLLAQGMEGRPVDALSGLLAHADMRVRQEAQF
                     ELAGRGDEGWRILAQIAGSSGQTLPRIHAIWGLGQAARTSRAGRSDALWDAIGGLLAD
                     PDPEVRAQTAKLVGDAALTKSLNRLIALLEDSSPRVRFFAAAALGRLGRSEAVEPLLR
                     MVRANGGNDPYLRHAGVIGLVGSGKTEAWSQAARDQSPQARMAILLAMRRREDPEIAL
                     FLSDADPLIVLEAARAIHDVPIPAALSSLAKLAIASTAPVPLLRRVLNANFRLGQPEN
                     AAVLAEVAARSELPEAARVLALEMLASWATPSGRDRVLGLWRPIPPRSPGLGIDALRP
                     RLAAILASGPANVRTAAAAAAGTMGIKEVGARLAALVTDRGLADKTRAEALKALDQLD
                     YPRRIEPAQQALVMPGYRSRTEALRILAKVDPGTALKPLRDRLEHGSTAERQGAMAVL
                     AAMPGEPARDELSGWVDRLIAGKVATEIQLDVIEAAKKRPETDFQQKIDRYESSKPKD
                     DMLAPYREVLTGGDAQRGMTVFTTKAELECVRCHKVRDSSGEPVGGEVGPELSGIGNR
                     QTRAYLLESIVDPNKQIAQGFESVVLATNDGQVHTGVLRGEDDKEVRLIMADGKPDVV
                     RKELIEERKRGPSAMPNDLARKLSRAELRDMIEFLANLKGK"
     gene            complement(4153..4398)
                     /locus_tag="VHS97_06560"
     CDS             complement(4153..4398)
                     /locus_tag="VHS97_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447897.1"
                     /translation="MHQVRWVAGEVVLAFTLAGCGESPPESGPVEFKATNSPEIEAFA
                     KRLSENAKGGKGAMKKPEAASKPGETKPAADGKAESK"
     gene            complement(4569..>4752)
                     /locus_tag="VHS97_06565"
     CDS             complement(4569..>4752)
                     /locus_tag="VHS97_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEX3447898.1"
                     /translation="TYGGWGTFMDALPPSSNHPGGVNIAFADGSVRWVKNSVTPNTWW
                     SLGTRNGQEALSSDAY"
BASE COUNT          899 a         1481 c         1459 g          913 t
ORIGIN      
        1 accttcagtg tttgggcgca gccatccgcg ccgggggcgg cgccgtggtc aagacggtgg
       61 gggaaggcgt ggtggcttcg tttgccgatg taaccagtgc ggtaaagacc gcgctcgatc
      121 tgcttcctca acttgcgagg ggcgcggcaa cgcgcccgct gcgtctgcgg gtcgcgattc
      181 atcgcggcac gacggtggcc gccactctca acgatcaact cgactatttc ggcacggcgg
      241 cccgccagac aattcaaatt ctcgacgatg cgcgcgggga cgaactcgtc ttgacccagg
      301 ctgtcgccgc cgatcccgag gtcgccggcc tgctggccac tcggcagatc gaaggcgaag
      361 tcgtgccatc ccgtctcgca gggcataacc atctcatccg cgtgcggctg gacgcgcagg
      421 tggccggcga aaaaagttag ccaacgatga tttcgcaccc gccctcggcc cgcaagattt
      481 ttcctcaatt attaagtgaa tatactatat atactccggc gggatcatcg cgcgagctct
      541 cttgcctttt gatggaggat ttggcttccc aatcggcgta agacaaacgt atctttaaca
      601 tgactgtttt agtcttcgct cgcggaaagc gagcccaccc gaacgttggt cccgcctcaa
      661 tcaatctctc cgctgagctc agacgacttc atgaaaaatc ttgtgccatt caataaagcc
      721 gccgcgtggg catgggtatt tgctgtcgcg ctggccgcgg acgtgcctgg tttcgcggat
      781 gatgagggta aaacgacccc gtctggctca cagtcacagg catacaatcc gcccgtcgct
      841 ccggcctccg atgaagggct gcgagcgatg aggtcgttcc gggtgccggc ggaattgaac
      901 gtggagcttt tcgcggctga gccgcatctc gccaatccgg tctcattctg tattgacgaa
      961 aagggtgttt tctatgtcgc cgaaacattc cgccacgggg caggtgtgac cgatacccgt
     1021 ggtcatatga actggttgga cgacgacctg gcatgccgtt cagtcgccga cagagtggcc
     1081 atgtacaaga aattcctggg gaaggagttc gccggataca atctgcaaca cgagcgcgtg
     1141 aggcgcattg tcgaccgtga tggtgacggc aaagcggacg ccgccacgat cttcgccgac
     1201 ggattcaatg acccggccgc cggcattggt gccggcgtgc tggcgcgcaa agacaacgtt
     1261 tggtttacgt gtattccctg gttgtggaag ttgcgcgaca cgaacggcga cggccgtgcc
     1321 gacgaccgca tcctcctgca cgagggttac ggtattcacg tcggcttcct gggacacgac
     1381 ctgcatggcc ttcggttcgg gccggacggc aggctttatt tcagcatcgg cgaccgaggc
     1441 ttcaatgtga cgacccgtga ggggcagcga ttgaccgtcc ccgacacagg ctcggtcttg
     1501 cggtgcaacc ccgatggtac cgaactggaa gtgttcgcga ccggattgcg caatcctcag
     1561 gagctggcgt tcgacgagta cggcaacctg tttaccggcg ataacaattc cgatagcggc
     1621 gaccgcgcgc gctgggttta cgtagtcgaa ggcggtgaca gcggctggcg gatcggctac
     1681 cagttcatcg aagcgccgat cagtcgaggg ccgtggaacg aagagagact ctggtatccc
     1741 cccttcgccg gccaggcagc gtatatcatc cctccaattg ccaatatcgc cgatggtcca
     1801 tccggtctaa cctacgaccc gggcgtcacg ctgcttcccg cgcaattcaa gaatcatttc
     1861 ttcctcgtcg acttccgcgg ctcgagcggt caaagcggta ttcgcacctt ttcgctccag
     1921 cccaaggggg cctcgtttca gcttgtcaat ccgcggcaat tcgtgtggtc ggtgctcgcg
     1981 acggatgccg actttggtcc cgacggcgca ctttatttct gcgactgggt cgagggctgg
     2041 gaaaaaacca ataagggtcg aatctaccgt gtgatggata gagcgcgacg tgatctaccg
     2101 caggtcctgg aggtcaaaac actgctggcg cagggaatgg agggacggcc cgtcgatgca
     2161 ctgtccggac tgctcgcgca cgccgacatg cgcgttcgcc aggaagcgca attcgagctg
     2221 gccggccggg gcgacgaagg gtggaggata ctcgcgcaaa tcgccgggtc gagcggacaa
     2281 acgctgcccc gaatccatgc gatctggggc ctgggacagg ccgcgcgcac ctcgcgcgcc
     2341 ggccgctctg acgccctatg ggatgcaatt ggcggcttgc ttgccgaccc cgaccccgag
     2401 gtgcgcgccc agacggccaa gttagttggt gacgcagccc ttacaaaaag cctcaaccgg
     2461 ctgatcgcgc tgctcgaaga ctcgagtccc cgcgtccgct tcttcgccgc ggccgccctg
     2521 ggcagactag gccgttccga ggcggttgag cctcttttgc gcatggtccg cgccaatggc
     2581 ggcaacgatc cgtacctgcg ccacgccggt gtcatcggcc tggtcggatc aggaaaaaca
     2641 gaggcgtgga gtcaagccgc gcgcgaccag tcgccccagg cacggatggc gatactcctg
     2701 gccatgcgcc gtcgcgagga tcccgagatc gctctcttcc tgagcgatgc cgaccccctg
     2761 atcgtgctcg aggccgctcg ggcgatccat gacgtcccga tacccgcggc tctgtccagc
     2821 ctcgccaagc ttgcaatcgc ctcgaccgcg ccagtgcctc tcttgcgccg ggttctcaat
     2881 gccaacttcc ggctgggcca gcctgagaat gctgccgtcc tggctgaggt cgcagcgcgg
     2941 tcagagctgc ccgaagccgc gcgcgttctc gcgctcgaga tgctggcgag ttgggcgacg
     3001 ccgtcgggac gcgaccgcgt gctgggactg tggcggccga ttccgccccg ctcgccgggt
     3061 ttgggcattg atgccttgcg cccccggctc gccgcgattt tggcgtcggg ccctgccaac
     3121 gtccggactg cggctgcagc tgccgccggg acgatgggaa tcaaggaagt cggcgctaga
     3181 ttggcggcac tggtgaccga tcgcgggctt gccgataaga cacgggccga ggccttgaag
     3241 gccctcgatc aactggacta cccgcggcga atcgagcctg cccagcaagc cttggtgatg
     3301 cccggataca gaagtcgcac cgaggccctc cgcattcttg ccaaggtcga tccagggacc
     3361 gcgctcaagc cgttgcgcga ccggctggaa catggttcga ccgcagagcg tcaaggagcc
     3421 atggcggtgc tggccgccat gccaggcgag ccggcgcgcg acgaactttc cggctgggtg
     3481 gaccggctga tcgccggcaa ggttgctacg gagattcagc tcgacgtgat cgaggccgca
     3541 aaaaaacggc cggagaccga tttccaacaa aagatcgatc gctacgaatc gtccaagccc
     3601 aaagacgaca tgctggcccc gtatcgtgaa gttttgacag gtggggacgc tcagcggggc
     3661 atgactgtct tcacgaccaa ggccgagctc gaatgcgtgc gctgccacaa ggtcagggat
     3721 tcctcgggcg agccagtggg gggcgaagtc ggccccgagc tttccggcat tgggaaccgc
     3781 caaacacgcg cctatttgct cgaatcgatt gtcgatccca acaagcagat cgctcaagga
     3841 tttgagtccg tcgtcctggc cacgaacgat ggccaggtgc ataccggcgt tctccgcggc
     3901 gaggacgaca aggaagtccg cctgatcatg gccgacggta aacctgacgt tgtgcgcaag
     3961 gaattgatcg aggagcggaa gcgcggtccc tcggcgatgc ccaatgacct ggcacgcaag
     4021 ctctcaagag ccgagctgcg cgacatgatc gagttcctcg ccaatctgaa aggcaagtaa
     4081 actgcccatc cggcgtcgga atacgagcag tcgatcaccg caggcgcagc ggcgagaatt
     4141 tcacgaccat tcctacttcg actccgcctt gccgtcagcc gcgggtttgg tctcgcccgg
     4201 tttgctcgca gcctctggct tcttcatggc acccttgccg cccttggcgt tttccgacaa
     4261 cctcttggcg aatgcctcga tctcgggaga attcgtcgcc ttgaattcca cgggccctga
     4321 ctccggtggg ctttccccac aacccgcaag tgtgaaggcc aatacgactt ccccggccac
     4381 ccagcgtact tggtgcatcg atgagttctc ctgtgtcgaa gtcaaaacct acagttgacc
     4441 tttttatgag cagctttgtc ggacatgagt gaactggcta acacacccag tctctttcca
     4501 ctggccaccc gacctttgga gtgagagccg aggacggctc caggccgtcc tcgaccggtc
     4561 aatctcgatc aatacgcatc agagctgagg gcttcctggc cgttgcgagt gccaagcgac
     4621 caccaggtgt taggagtcac cgaattcttg acccagcgta ccgacccatc ggcgaatgca
     4681 atgttgacac cgccgggatg attactgctc ggaggcaaag cgtccatgaa cgtaccccag
     4741 ccaccgtagg tc
//
LOCUS       DATAQL010000381         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1105274, whole genome shotgun sequence.
ACCESSION   DATAQL010000381 DATAQL010000000
VERSION     DATAQL010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105274"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VHS97_06570"
     CDS             <1..196
                     /locus_tag="VHS97_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447899.1"
                     /translation="RMVGYAVATGTTLLVAALAFFLFGKGVAAAIIAAWLTLAATGAG
                     LVALLAHTFDRFDVTQDTAA"
     gene            complement(276..>1595)
                     /locus_tag="VHS97_06575"
     CDS             complement(276..>1595)
                     /locus_tag="VHS97_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX3447900.1"
                     /translation="GLAQTRRRVGMGVSALLACWLVWSLTPGVPVVRAPEGSARVVEA
                     GLTLFEHDWTPGDSLAAGDGLGPVFNERSCVACHFQGGVGGGGDSKHNVKSFEVHPVP
                     GRPEVMGGILHGFAISKDCRESAKGLHDFFAVVPGGLKIVGGCTIETRDFDPVRIHSV
                     NATALFGDGWIDRISGKSILHQSRRKSFGAIGRELVGDLGGVSPGRPRILADGRIGKF
                     GWKAQFATLEEFVASACANELGLGNPLMEQAKPWAHVSYSKVQPDLDKTQFRALVEFV
                     DTLPRPTEVMARDANERSLSERGKLLFEQVGCAACHAPEIGGVAGIYSDFLLHRVSNP
                     STAGSGYSEVPSVPLPHGHPLPDEWKTPPLWGVADSAPYFHDGGSPTLEAAILRHHAD
                     AAPVTRAYESLPSVDRQAIIAFLKSLRAPSDAKPADRGPSRVIAMAH"
BASE COUNT          310 a          535 c          471 g          279 t
ORIGIN      
        1 gcgaatggtc ggctacgccg tggccaccgg aaccactttg ctggtggccg ccctcgcgtt
       61 tttcctgttc ggcaagggag tcgccgccgc aatcattgcg gcctggctga ccctcgcggc
      121 caccggggcc ggcctggtcg ccctcctggc gcacacgttc gatcggttcg atgtcacgca
      181 ggacactgcg gcgtgatctc cgttgcggtc gatggccggc gtcaagcgct acccgcccga
      241 cgagcggcca tcgacctgaa tcgacaccag cgcgatcaat gtgccatggc gatcacccgg
      301 ctcgggcctc ggtcagctgg cttggcatcc gagggagccc gcaagctctt caagaaagcg
      361 ataatcgcct ggcgatcgac ggacggcaga gattcgtacg cacgagtcac cggtgcggcg
      421 tcagcatgat gtcggaggat ggcggcctcc aaagtcggcg atcccccgtc gtggaagtag
      481 ggagcgctgt cggccacgcc ccaaagtggc ggagttttcc attcatccgg cagaggatga
      541 ccgtgcggca gaggaacact tgggacttcg gagtagccgc tgcccgcagt gctcgggttg
      601 ctgactcgat gcagcaggaa gtcggagtaa atgccggcca ctcccccaat ttccggagca
      661 tgacacgccg cgcatccgac ctgctcaaat aacaatttgc cgcgctcgga cagtgatcgc
      721 tcgttcgcgt ctcttgccat gacctcggtc gggcgaggca aggtatcgac aaactccacg
      781 agcgcgcgga actgggtctt atccaggtcg ggttggactt ttgagtacga aacgtgagcc
      841 cagggcttgg cctgctccat caagggattg cccagaccga gctcattggc acaggcggac
      901 gcgacgaact cttccagagt cgcgaactgc gccttccagc cgaattttcc gatccgtcca
      961 tcggccagaa tgcgcggccg gcctggactc acgccgccca aatcgccgac aagctcccta
     1021 ccgatcgcac cgaatgactt gcgccggctc tggtgcaaga tgctcttgcc agagatccgg
     1081 tcgatccatc cgtctccgaa taaggccgtc gcgttgacgc tgtggatgcg gaccggatcg
     1141 aaatcgcgcg tctcgatcgt gcacccgccg acgatcttca gacctccggg cacaacggca
     1201 aagaagtcat gaagcccttt ggcgctttca cgacaatctt tcgagattgc aaagccatga
     1261 agtatccctc ccatcacctc agggcgaccc ggtacgggat gaacctcgaa ggatttcacg
     1321 ttgtgcttcg agtcgcctcc gccgccgacg cccccttgaa agtggcacgc cacgcacgac
     1381 cgttcgttga ataccggccc cagaccgtcg ccggcggcca gcgaatcgcc tggtgtccaa
     1441 tcgtgctcga acaacgtcag accagcctcg acgacccgcg cgctgccctc tggggcacgc
     1501 acgaccggaa cacccggtgt cagagaccag accagccaac aagccaggag ggccgacacc
     1561 cccataccaa cccgccgacg agtttgcgct aaccc
//
LOCUS       DATAQL010000382         5610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_495692, whole genome shotgun sequence.
ACCESSION   DATAQL010000382 DATAQL010000000
VERSION     DATAQL010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495692"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1797)
                     /locus_tag="VHS97_06580"
     CDS             complement(58..1797)
                     /locus_tag="VHS97_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3447901.1"
                     /translation="MKILIAEDQPPAALFLRRMLETMGHEVVVAPDGEAAWQIVRDGN
                     APILISDWMMPRLDGLDLCRRIRAAGGDRYTYVILLTSRDRREDRLEGLRAGADDFLT
                     KPPYPDEIALRVEIAERILAVHDHLARRNLQLAHQARHDELTGLPNRSHLQHELERRL
                     NGRSSANPFALLLIDLDRFKEINDALGHHYGDLLLQAIAPRLRDVIGDSGIAARIGGD
                     EFGILVPGADGASAVRVAESVLAALRQPIVIKGRALDVGASIGVALCPGHADDPIAML
                     QCADIAMYAAKRNRVGCLVYAAERPEFQPVRVALIGELRRGIEQNQLLLHYQPKINLR
                     TRAVEGVEALVRWLHPREGLLPPSQFIELAEETGLIKPLSLWTLQTALLQCRVWRQEG
                     SALNVAVNLAADVLREPDLLEMIVANLQISDTLPGWLTLEITESAVMADPKSAKAMLG
                     RLRGMGVRIAIDDFGTGYSSLGYLRDLPVDEIKIDRSFVQRVTSGDPNASIVRSVIDL
                     GRNLGLKVVAEGAEDKETVELLESLGCDYVQGFYFSKPLAPADFAAWLARSQVNGLGG
                     MHSIPRQTTVRVT"
     gene            complement(1794..5342)
                     /locus_tag="VHS97_06585"
     CDS             complement(1794..5342)
                     /locus_tag="VHS97_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3447902.1"
                     /translation="MEEVNLTSDSPPISSAAMSRRAEELFEQRRRDLSRTTDRLFSGL
                     LAFEWWAAILTALVIAPRAWSGTTSQIHPFIWAATLLGGAIVSLPVMLAWSQPGRVLT
                     RHLIAVTQMLIGTLMIHLTGGRIETHFYIFGSLAFLAFYRDWKVLVSASVVVAADHVI
                     RGLFWPQSVYGVFLIEPWRWAEHAGWVAFEDVFLIWSCCKSIGEMWAIAERQAALELL
                     HSQVERQVAVRTAELRESEARKTGIVEVALDAIVTADQNGRVIEFNPAAERIFGYSRA
                     QVYGKPLAELIVPPALRDDHCKGLNGYLTTGEGSVIGKRIEITAYRAEGTEFPAELAV
                     CLIERSDPPVFTAYLRDLSRQKEAEDALAERARLADLTADVAIALAQGVNATGILQKC
                     TEAIVRQLDAAFARIWTLNDENVLELRASAGMDTHLDGGRSPLRVGDFDIGLIAQERQ
                     PLLINTVLTDPRVHDKEWAEREGLMAFAGQPLIVEKRLVGVIALFSRQPLSVAAFKAV
                     GVVADTIAVGIVRLQAQIEQEKAKLAAVAASRAKTEFLANMSHEIRTPMNGIIGMTEL
                     ALDTPLTPRQREYLSIVKSSSESLLTVINDILDFSKIEAGKLGLDPAPFALRETVERT
                     LNILALRAHAKGLELACRIAPEVPDALVGDDDRLRQVLMNLVGNAIKFTEHGEVIVNV
                     ALDEEPGVEGLVLRFGITDTGIGIPEDKLRTIFEPFEQVDGSTTRRFGGTGLGLTISS
                     KLVEMMGGRIWIESNPGTGTTFWFTTTLVLQPKTANSRGRIDSGLSRLDGLRVLIVDD
                     NATNLLILSEVLSNWGAKPVAVTSGPAALDRLRFAAAKGQPFVIALIDGMMPEMDGLD
                     LARHIRSEPTIAGVLLLMLTSSGPPEDENLCRELRIAVCLTKPVRQSELLDALDRALA
                     PTGQPNVLPTECRPSEGRVDMIPADGGLHVLLAEDHPVNQKVAVRMLEKMGHSVIVAS
                     DGLEALEALEAGDFDVVLMDLQMPRMDGFEAVRAIRQREAERGNHMHVLALTAHAMQG
                     DRERCLAAGFDNYLAKPVRQTELEAALSAIHRLAKAADHRVMEGLNEVCGGDDEFARE
                     LSQSFLESAPRCLAGIEEALRSEDAIRLAQEAHGLKGISRTIGALDQAVACEALEAAA
                     RRGDLALASSEAARVQMAWERLRTVLERLAYSQVTT"
     gene            complement(5330..>5610)
                     /locus_tag="VHS97_06590"
     CDS             complement(5330..>5610)
                     /locus_tag="VHS97_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447903.1"
                     /translation="VHLIDDSGGEEAARFGARTSGLVALYAPDGRLHFRGGITGSRGH
                     EGDNAGQQALLGLIQGNPSSLPCETPVFGCPLFPARSISAESPIPWKK"
BASE COUNT         1099 a         1743 c         1688 g         1080 t
ORIGIN      
        1 aggaagaaat tccgacaaca attccagctt gtgtttgagc cacggagcgc ttcccactta
       61 agtgacccga accgtcgtct gccgcggtat ggagtgcatc ccgccgagac cgtttacttg
      121 tgaccgcgcc agccaggccg cgaaatcagc gggcgcgagg ggcttggaga aataaaaccc
      181 ctggacgtag tcacagccga gagactcaag caactcgacg gtctccttgt cctccgctcc
      241 ttcggccacg accttcaggc cgagatttct tccgaggtcg atcaccgatc tcacgatgct
      301 ggcgtttggg tcgcccgagg tcacccgttg gacgaacgac ctgtctatct tgatttcgtc
      361 aactgggaga tccctcaggt atccgagtga ggagtaaccc gtcccgaaat cgtcgatggc
      421 gattcgcacc cccataccgc gcaaccgccc tagcatcgcc ttggcgctct tcggatcggc
      481 catgacggcg ctctccgtga tttccaaagt aagccatcct ggcaatgtgt cagagatctg
      541 aagattggcg acgatcattt cgaggaggtc cggctctctc aacacgtcag ccgccaggtt
      601 aaccgcgacg ttcaaagcgg acccttcctg acgccaaacg cggcattgca ataacgccgt
      661 ttgaagagtc caaaggctaa ggggcttgat cagtcccgtc tcttcggcga gctcgatgaa
      721 ctgacttggt ggaagaagcc cctctcgcgg atggagccag cgcaccagag cctccacacc
      781 ctcgacagcg cgtgtacgca ggttgatttt tggttggtaa tgcagcagga gctgattctg
      841 ctcgatcccc cgacgtagct cacctattag tgcgacacgg acaggttgga actcagggcg
      901 ttccgcggcg tagacgaggc agccgacccg atttcgtttc gctgcataca tggcgatgtc
      961 ggcgcattga agcattgcga tcgggtcatc ggcgtggccc gggcagagcg cgacgccgat
     1021 gcttgccccc acgtcaaggg cccgtccctt gatcacaatc ggttgccgta gcgcggcaag
     1081 gaccgactcg gcaacccgca ccgcgctcgc gccatcggcg cccggtacga ggatgccgaa
     1141 ctcatcgccg ccgatccggg cggcgatccc ggagtcgcct atgacgtcac ggagccgcgg
     1201 ggcaatcgcc tgaagaagca gatcgccata gtgatggccg agggcgtcgt tgatctcctt
     1261 gaaccgatcg aggtcgatca gaagcagggc gaacggattt gcgctggatc gtccgttaag
     1321 acgccgctcc aattcgtgct ggagatggga acgattcggc aagccggtca gttcgtcatg
     1381 cctggcctgg tgtgccaatt gcagattccg ccgcgccaga tggtcgtgga cggccagaat
     1441 acgctcggcg atttcaacgc ggagggcgat ctcatccggg tagggtggct tggtaaggaa
     1501 gtcgtcggcg ccggcgcgaa gcccttcgag tcgatcctcc cgccggtcgc gcgaagtcag
     1561 caagatgaca taggtgtaac gatcgcctcc ggcggcgcga atccggcgac agagatcgag
     1621 gccgtcgaga cgcggcatca tccagtccga gatcagaatt ggcgcatttc cgtcgcggac
     1681 gatctgccag gccgcctcac cgtcaggggc gacgacgact tcgtgcccca ttgtctccaa
     1741 catccgccgc aaaaagaggg ctgccggcgg ttgatcttca gcgatcaata tcttcatgtt
     1801 gttacctgag aatacgccaa tcgctccaac accgttcgca atctctccca tgccatttgg
     1861 acgcgcgccg cctccgagct agccagtgca aggtctccac gtcgggccgc cgcctccaat
     1921 gcctcgcagg caaccgcctg atcgagggcg cctatcgtcc ggctgatccc cttcaagcca
     1981 tgagcctcct gtgccagcct gattgcatct tccgatcgca gcgcttcttc gatcccggcg
     2041 aggcagcgcg gtgccgattc caggaacgac tgggagagct cgcgagcgaa ttcgtcgtcg
     2101 ccgccgcaga cttcgttcaa tccctccatt actcgatggt cggcggcctt ggccaggcga
     2161 tggatggccg agagagcggc ctcgagttcg gtttggcgga cgggtttggc gaggtagttg
     2221 tcgaagcccg cggcgaggca gcgctcacgg tcgccttgca tggcgtgggc cgtcagggcc
     2281 aaaacgtgca tgtggtttcc ccgctcggcc tcgcgctgtc gtatggcccg gactgcctcg
     2341 aaaccgtcca tcctgggcat ttgcaagtcc atgaggacga catcgaagtc accggcctcg
     2401 agcgcttcga gcgcctcgag tccgtcagag gcaacgatca ccgaatggcc catcttttct
     2461 agcatgcgta cggccacctt ctggttgacg gggtggtcct cggctagcaa aacgtgcaag
     2521 ccaccatccg caggtatcat gtcgacgcgg ccctcactcg gcctacattc ggtgggaagt
     2581 acattgggct ggccggtggg tgccagggct ctatcgagcg cgtcgagcaa ttcagattgc
     2641 cggaccggct tggtcaagca aacggcgatc cggagctcgc ggcagaggtt ttcgtcttca
     2701 ggtgggccgc tcgaggtcag catcaaaagc agtacgccgg cgatcgtcgg ctcgctgcga
     2761 atgtggcggg cgaggtccag tccgtccatc tcgggcatca ttccgtcaat caaagcgatg
     2821 acgaatggct ggccttttgc ggcggcaaat cgcaacctgt ccagggctgc cggtccgctc
     2881 gtgacggcga ctggcttggc accccaattc gacaggacct cactgagaat cagcagattg
     2941 gtggcgttgt cgtcgacgat caggacgcgt aggccatcaa gccgcgacaa ccccgagtcg
     3001 atccggccgc gagaattcgc agtcttaggc tggagaacca gcgtggtcgt gaaccagaaa
     3061 gtagtacccg tgcccgggtt gctttcgatc cagatgcgcc ctcccatcat ctccaccagc
     3121 ttggacgaga tcgtcaggcc gagtcccgtg ccgccaaatc gccgggtcgt tgagccgtcg
     3181 acctgttcga acggctcgaa gatcgttcgc agtttgtcct cgggtatacc gatcccggtg
     3241 tcggtgatac cgaagcgcag gacgagtcct tcaacgccgg gctcctcgtc cagtgccacg
     3301 ttgacgataa cttcaccgtg ctcggtgaac ttgattgcgt tgccgaccaa gttcatcagg
     3361 acttgccgca gacggtcgtc gtctccgacc agtgcgtcgg gaacttcggg ggcgatgcgg
     3421 caagccaact ccaggccctt ggcgtgcgct cggagcgcca atatgtttag cgttctttcc
     3481 acggtttcgc gcagcgcgaa cggtgccggg tcgagtccca atttgccagc ctcgatcttg
     3541 gagaagtcga gaatgtcgtt gatgacagtc agcagcgact cggacgagct tttcactatg
     3601 ctgaggtact cgcgctggcg cggcgtcagc ggcgtgtcga gcgccagttc ggtcatgcca
     3661 atgattccgt tcatgggcgt gcgaatctcg tgactcatgt tggccaggaa ttcagtcttg
     3721 gccctgctcg ccgccacggc ggccagcttg gccttttcct gttcaatctg ggcttgcaac
     3781 cttacaatcc ccacggcaat ggtgtccgcc accacaccca cggccttgaa cgcagctacc
     3841 gaaagcggtt ggcgcgagaa cagcgcgatg actcccacaa gccgtttctc gacaatcaac
     3901 ggttggccgg caaaggccat caatccttct cgctccgccc actctttatc gtgaacacgg
     3961 gggtctgtca ggactgtatt gatcagaagg ggctgccgct cctgagcgat caagccaatg
     4021 tcgaagtctc cgactcgcaa aggcgaacgg ccgccgtcga gatgtgtgtc catcccggcg
     4081 ctcgccctta attccagcac attctcgtca ttgagcgtcc agattcgcgc aaacgcagcg
     4141 tccaactgtc ggacgatggc ttcggtgcac ttctggagga tccctgtggc attaacgcct
     4201 tgggccaagg cgatggccac gtcggcagtg aggtcggcga gacgcgcccg ttcggccagt
     4261 gcgtcctcgg cctctttctg cctggaaagg tcgcgcaaat aggcggtgaa gaccggcgga
     4321 tcggaccgct ctataagaca gacagccagt tcagcaggaa actcagtacc ttcggcacgg
     4381 taggcagtga tttcgatccg cttgcctatg accgaccctt cgccggtggt taggtacccg
     4441 ttcaatccct tgcaatggtc gtcgcgaagc gcaggcggga cgataagctc agccagcggc
     4501 ttgccgtaga cttgggctcg gctgtagcca aagattctct ccgccgcggg gttaaactcg
     4561 atcacgcgtc cgttctgatc ggccgtcacg atcgcatcca gcgccacttc gacgatgcct
     4621 gtcttccgcg cctcgctttc gcggagttcg gcagtccgca cggcgacctg gcgttccact
     4681 tgactgtgca gcaactccaa ggcggcctgc cgctcggcaa tggcccacat ttccccaatg
     4741 ctcttacagc acgaccagat gaggaacacg tcctcgaaag cgacccagcc ggcatgttcg
     4801 gcccaacgcc acggctcaat caggaacaca ccgtacactg attggggcca gaagagcccg
     4861 cgaattacgt gatcggcggc gacgacgacc gacgctgaga cgaggacttt ccagtcgcgg
     4921 tagaaagcga gaaaggcgag cgacccgaag atgtagaaat gggtctcgat ccgtccgcca
     4981 gtgagatgga tcatcagggt gccgatgagc atctgagtca ccgcgatcag atgccgggtc
     5041 aagaccctgc caggctgaga ccaggccaac ataaccggta ggctgacaat cgctccacct
     5101 agaagagtcg ctgcccatat aaacggatgg atctggctgg tcgtgcccga ccaggctcgt
     5161 ggcgcgatca caagcgctgt caggatcgcc gcccaccact cgaacgcgag cagccccgag
     5221 aacaagcgat cggtcgtccg actgaggtcc cgtcgccgct gctcgaaaag ctcctcggca
     5281 cgtcgcgaca tagccgccga tgagatgggg ggcgaatcgg acgtcagatt cacttcttcc
     5341 atgggatggg actttcggca gagatggaac gagccgggaa gagcggacag ccgaagacgg
     5401 gagtttcgca gggaagcgac gacggattgc cctgtatcag gccaagcagg gcctgctggc
     5461 cggcgttatc cccttcgtgg ccccgggaac ccgtgatccc gccgcggaaa tggagccgcc
     5521 catctggagc gtataatgca acaagaccgg aagtccgggc gccgaagcgg gccgcttcct
     5581 cgccgccgga atcgtcgatc agatgaacac
//
LOCUS       DATAQL010000383         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5075618, whole genome shotgun sequence.
ACCESSION   DATAQL010000383 DATAQL010000000
VERSION     DATAQL010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5075618"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="purN"
                     /locus_tag="VHS97_06595"
     CDS             <1..615
                     /gene="purN"
                     /locus_tag="VHS97_06595"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX3447904.1"
                     /translation="IRLAVCVSGEGTTLQNLIDQIRLRRLKAELVQVVASRPRIGAIA
                     RAEAAKIPLALANYNASSRTEFSNSVFEPIRHSKADLVILAGFLALVKIPPDYKGKVF
                     NIHPSLIPSFSGKGFYGSKVHEAALAMGVKLSGCTIHFADDSYDTGPIIAQRAVPVLE
                     NDTIDTLAARVFIEECKALPEAISLYAEGRLKLDGRRVRVAPQA"
     gene            complement(644..1336)
                     /gene="bshB1"
                     /locus_tag="VHS97_06600"
     CDS             complement(644..1336)
                     /gene="bshB1"
                     /locus_tag="VHS97_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX3447905.1"
                     /translation="MLDALVVAPHPDDAELGMGGTIVRLMQQGWKIGILDLTSGEPTP
                     LGSLERRAAETAAANAALGNPWRKNLGLPNRSLEPTLLHRRAIAAIFRQVRPPLVFAP
                     YWEDAHPDHTAATKLVEDARFWSKLTKSDIGGEPFHPARILYYFSVHLRIIERPSLVL
                     DISDQLDVKVAALRCYRSQLVDNQPDGRPGVIETVCDRTRYWGQLAAVRHAEPFASRE
                     PVGLRGLDQLLL"
     gene            1601..1909
                     /locus_tag="VHS97_06605"
     CDS             1601..1909
                     /locus_tag="VHS97_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX3447906.1"
                     /translation="MPRFVKMATLTELPAGAALEVEHEGRVYALYNIDGVVSAIDGIC
                     PHQGGPLADGQVDGTTVTCPWHGWQFDIRTGSTPLGPKIKQAVYEVKIEGEDVLVAVP
                     "
     gene            2012..3004
                     /locus_tag="VHS97_06610"
     CDS             2012..3004
                     /locus_tag="VHS97_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3447907.1"
                     /translation="MTRFRSFRNSDPPFLAELWNQAVPSLGCVRPVRVHELDSHALGT
                     VLFDADGLIVAETDGRIVGFVHAGFGPELPVESTQPFDVCHEIGTISMLCVEASLDDP
                     GLLAGLIDAAERYLRSRGAKVLYAGGLFPLNPFYWGIYGGSEGAGVLAGHDAFHRVLR
                     ASGYEPVSTTVLLEADLAVPEPRDPRVPLIRRHTQIEVFDDALPSHWWQNLALGDFEL
                     MTVRLLMKDDGIPVARAQAWDMGWFGRDDERTRIGLINLEVPPQYRRKGYGRFLVSEI
                     FRRARENMIAAVAVATSSSNQPALALYASLGFHPIDESTLYRLATDGHERSGHA"
     gene            3133..3450
                     /locus_tag="VHS97_06615"
     CDS             3133..3450
                     /locus_tag="VHS97_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447908.1"
                     /translation="MARRTLNRRELRAEAEAAEAMGISSEPDSKKAPRAPRQPSLRPK
                     PAAAPRMRVVWAVCDVGGRTVATFDYPCKVEAEALVAKLKSQGKGMHFVRSVKEPIQH
                     DHS"
     gene            complement(3515..>3890)
                     /locus_tag="VHS97_06620"
     CDS             complement(3515..>3890)
                     /locus_tag="VHS97_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3447909.1"
                     /translation="VYVASKHAVNGLVKSLRYELWGTGVRVWAACPGRTESEFRQVAL
                     GARGGEVGRLPTGEPTAKIVRAIVRGLDRRSAFVLPSWRAWSIVTLAHWLSAPFDWIM
                     CRWSPGSYKDVLAQPATHADTE"
BASE COUNT          778 a         1172 c         1159 g          781 t
ORIGIN      
        1 atccgtttgg cggtgtgcgt ttcgggcgag ggaacgaccc tccaaaacct gatcgaccag
       61 attcgattgc gtcgcctcaa ggcggaattg gtccaggtcg tggcgagccg gcccagaatc
      121 ggtgccatcg cacgggccga ggcggcgaaa attccgcttg cgctggccaa ttacaacgcc
      181 agttcacgga ccgagttcag caattccgtc ttcgagccga ttcgtcacag caaggccgac
      241 ctcgtaattt tggctggttt cctggcactc gtgaagatcc cgcccgatta caaaggcaag
      301 gtgttcaaca tccatccatc tctgatcccg tccttcagcg gtaagggctt ttatggatca
      361 aaagtccatg aagccgcgct tgctatgggc gtcaagctca gcggctgcac gattcatttc
      421 gcggatgact cgtacgacac cggacccatc atcgcgcagc gcgccgttcc tgtactggag
      481 aacgacacaa tcgacacgct cgctgcccgt gttttcatcg aagaatgcaa agcgcttccc
      541 gaagcaatct cgctctatgc cgaaggccgt ctgaaactgg acggcaggcg cgttcgagtc
      601 gcaccgcaag cttgacggaa ggctctcgcg cgatcaaacg acatcacaga agaagttgat
      661 cgagaccccg gagcccaacc ggctcacggc tcgcaaatgg ctcggcatgg cggacggcgg
      721 ccaactggcc ccagtaccgt gtccggtcgc agaccgtttc gatcactcca ggccggccat
      781 cgggctggtt gtcgaccaat tgcgagcgat aacaccgcag tgccgccacc ttgacatcga
      841 gttggtcgga aatgtccaag accaggctgg gacgctcgat gatgcgcagg tggacactga
      901 agtaatagag gatccgagcc gggtgaaacg gctcgccgcc gatgtcggac tttgtgagtt
      961 tgctccagaa tcgcgcatcc tcgaccagct tggtagccgc cgtgtggtcg gggtgggcgt
     1021 cttcccagta aggggcgaag acgagcggcg ggcgcacctg gcggaaaata gccgcgatcg
     1081 ctcgacggtg aagcaacgtc ggctcgagac ttcggttggg caggccgaga tttttccgcc
     1141 aggggttgcc cagggcggca ttggctgcag ctgtctcggc cgcccggcgc tccaggcttc
     1201 ccagcggcgt cggttcacca ctggtcaggt cgaggattcc gatcttccag ccctgctgca
     1261 tcaggcggac gatcgtccct cccatgccaa gctcggcatc gtctggatgc ggggcgacga
     1321 ccaaggcatc gagcatgcgt cgtctatccg aaaagtcgag tgaagggtta tcccatcaaa
     1381 ccactaagac gctagttgtt atccaactga gacacaaggc catcctcgca ctctcgcctt
     1441 gcgaccagtt gttcgatttg agaaaaaccg cccaatcgtg tagaaaagag gtgaacggtg
     1501 agaaattgga tggacctccc tgcgtttgca actgggtctg catgagtttg gtgagtgacg
     1561 tgggccggcc gagaatgcca ggggacccgg agtcaagcgt atgccacgat tcgtcaagat
     1621 ggccaccctt actgaacttc ctgctggagc cgcgttggag gtagagcacg aggggcgggt
     1681 ctatgccctt tacaatatcg acggcgttgt ttccgccatt gatggaattt gtccgcatca
     1741 gggaggtccg cttgcggacg ggcaggtcga cggaaccacg gtgacgtgcc cctggcatgg
     1801 ctggcagttc gatatccgga ccggcagtac tccgctgggg cccaagatta agcaggccgt
     1861 ctacgaagtc aaaatcgagg gagaagacgt gctagtcgcc gttccctgag cgatatcttc
     1921 gttcacatcg ccacgatctg gccgaccgct cggggcgggg gccgctggcg tttcctagtc
     1981 caacttgaat cgacctcggc gatcccaacc ggtgacgcga tttcgctcgt ttcgcaattc
     2041 tgatccccct tttctcgccg agctctggaa ccaggccgtt cccagcttgg gatgtgtacg
     2101 accggtccgg gtccacgaat tggacagcca tgcgctcgga acggtgctgt tcgacgctga
     2161 cggactgatc gttgccgaaa cagatggccg gattgtcggg ttcgtacacg ccggattcgg
     2221 tccggaactc cccgtcgaat cgacccagcc gtttgatgtt tgccatgaga tcggcaccat
     2281 cagcatgttg tgtgtcgagg cgagccttga cgatccgggg ttgctcgccg gcctgatcga
     2341 cgcggccgag cgctatctcc gctcgcgggg tgcgaaagtc ctttatgccg gcggtctatt
     2401 tccgctcaat cccttctatt ggggcatcta tggcgggagt gaaggggccg gagttctcgc
     2461 cggacatgat gcgtttcatc gcgtcctcag ggcgagcggt tacgaaccgg tgagtacgac
     2521 tgttctcttg gaggcagatc tcgccgtacc ggagccgcgc gacccccgcg tgcctttgat
     2581 acgccgccac acgcaaatcg aagtatttga cgacgcattg cccagccact ggtggcagaa
     2641 tctagcactc ggcgacttcg aactcatgac cgtgcggctg ctcatgaagg atgacggcat
     2701 accggtcgct cgggcgcaag cgtgggatat gggctggttt ggacgggatg atgagcgtac
     2761 gcggatcggc ttgatcaacc tggaagtccc accgcaatac cgccgtaaag ggtacgggcg
     2821 cttcctcgtc agtgaaatct ttcggcgcgc gcgcgagaac atgatcgccg cggtggcggt
     2881 tgccacttca tcctccaatc aacccgctct ggcgctctac gcctctctgg gctttcatcc
     2941 gatcgacgag tccactcttt atcgacttgc taccgacggt cacgagcgtt ccggccatgc
     3001 atgatcggcg aagttccctt ccaaagttgg tcttgctgag cttacggttc ttgttatcca
     3061 ctaacgatct cgatcttcgt cattaagcga cagcagaccg actatcaact tcagcataaa
     3121 gtcgagattc tcatggcacg acgtacactc aatcgacgag agctgcgggc tgaggcggaa
     3181 gccgccgagg cgatgggaat ttcgagtgag cctgactcaa aaaaggctcc ccgggccccg
     3241 aggcaaccct cactacgccc aaaaccggcc gccgcgccca ggatgcgggt agtgtgggcc
     3301 gtttgtgacg tgggcggacg aacggtggca acgtttgact atccgtgcaa ggtcgaagcc
     3361 gaggcactcg tcgccaagct gaagagccag gggaaaggaa tgcatttcgt acgatccgtc
     3421 aaggaaccaa tccagcacga ccactcctga ggcggccccg ggacctcttt tgctaccgag
     3481 taacagtcga tcgaagcgcg tctctcgatg tttgtcactc tgtgtcagcg tgggttgccg
     3541 gctgggcaag cacatcctta taagagcccg gcgaccagcg gcacatgatc cagtcgaacg
     3601 gggcggacag ccaatgtgcc agtgtgacaa tgctccaggc tcgccagctc ggtaacacaa
     3661 acgccgagcg acgatcgagg ccgcgtacga tcgcgcggac gatcttggcc gtcggctcgc
     3721 cagtgggcag cctcccgacc tcacctccgc gggcgcccag cgccacctgc cgaaactcac
     3781 tttccgttct cccagggcac gccgcccaga ccctgacccc ggttccccat aactcgtagc
     3841 gcaaactttt caccaggcca ttgactgcgt gcttgctggc aacataaacc
//
LOCUS       DATAQL010000384         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_799534, whole genome shotgun sequence.
ACCESSION   DATAQL010000384 DATAQL010000000
VERSION     DATAQL010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799534"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1761
                     /locus_tag="VHS97_06625"
     CDS             298..1761
                     /locus_tag="VHS97_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447910.1"
                     /translation="MIALRAYSRMHLIPKEALAQEMGGDALARERVLVVPSLVWAVWG
                     AMTVATLLFIWHYSRNIPFADDFSLVPVMTGHEPVSLRWLWSQHNEHRPIVPRLVMVG
                     LNRLISSDFRLGLYVNGALLSAAAASMLLLTRKLRGHTRLVDVVLPLSILNIGQAQTL
                     LLGLTMALTMTSLVSCELIRLAAAQNSRLGLPLTLKLGLFLVLLPLCGAGGLIMLPPL
                     LLWLACESIWGCRREGSPEKVKVARAIGVALLVVCLAVVTFYMLGYKSPPQNRPPTSF
                     VAAISTLLEYLSLVIWPNVLDYWRAAGLIVAFLVAATLVRLCLVGRRQPDDRVRAFAM
                     IAVILAMLCAAVAVGYSRSFIGPGGARVGRYVTTTSPLFCALYVAWLIHGSARTRRRV
                     HAALFVVVILTVPTNIKHGLRVGEWRRAVYTRLERDMKAHSTLSAVVKNAWPHLYPSK
                     EFIEVSFKMLKEAQVCRFGDLVYDRPANAPNGSSAIR"
     gene            complement(1808..>2222)
                     /locus_tag="VHS97_06630"
     CDS             complement(1808..>2222)
                     /locus_tag="VHS97_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3447911.1"
                     /translation="TLDPAQFLRRQVELLRSGGILVVNDHITDPDSQVAAHHGKIEVA
                     RDRTHTRNLTGGELVDLFVGVGLTDIRYVEEPFMLDFDEWFDRGSPSDTKDHVRELLR
                     SGPSIRSFRPELLDDGSIRIAGIRAIVRGVNPGRT"
BASE COUNT          372 a          659 c          683 g          508 t
ORIGIN      
        1 cggtgctatc ggctccggcg atttctgtct cggggtggcg ctggcgtgtt cttcgctgaa
       61 ccaatcttta gtcacgtatt actgataacc gatacacaca tgacagtagc aggcgcgcaa
      121 cgagccccgg agcggatacg cgcaagaacg acgtccagcg tcctgcccac gtaggggtca
      181 cggacccgaa gacgccgcgc gttgactctg cgtcaatagc tggaccaatc gtccagcaca
      241 gccgggtcgt cgcgtcagcc gaagagcgcc aggagctatc ttctccgtgg aagcgggatg
      301 atcgccctgc gcgcgtattc acggatgcat ttgataccaa aggaagccct cgctcaagaa
      361 atgggtggtg acgctttggc tcgcgaacgg gtgctggtcg tcccgagcct cgtctgggcc
      421 gtttggggcg cgatgacggt cgcgaccttg ctctttatct ggcactattc gcgtaacatc
      481 cccttcgcgg atgacttttc actcgttccc gtcatgaccg gacacgagcc ggtgagtctg
      541 cggtggctgt ggtcgcagca caacgagcac cgaccgattg ttccccggct ggtcatggtg
      601 ggcctgaacc gcctgatctc gagcgacttt cgattagggc tgtatgtaaa cggggcgcta
      661 ctttccgcgg cggcggcatc gatgctcctt ttgacgcgta agttgcgcgg gcatacccgt
      721 ttggtcgatg tcgtgctgcc gctgtcgatc ctcaacatcg gtcaggccca gacgctgctg
      781 cttggtttaa cgatggcttt gacgatgacc tccttggtct cttgcgagct aatcagactg
      841 gcggcggccc agaattcccg gctcgggttg cctttgacgt tgaagctcgg actcttcttg
      901 gtgctcttgc cgctgtgcgg ggcaggcggt ctgatcatgc ttcctcctct cctgttgtgg
      961 ctggcctgcg agtcgatttg gggttgcagg cgcgagggaa gtcctgagaa ggtcaaggtc
     1021 gcacgcgcca tcggcgtggc gctgctcgtc gtttgcctgg ccgttgtcac gttttacatg
     1081 ctgggctata agagcccacc ccaaaatcgg ccgcccacct catttgtggc ggccatatcc
     1141 actctcctgg aatacttgag tctggtgatt tggcccaacg tacttgacta ctggcgcgcc
     1201 gccgggctga ttgtcgcctt tctcgttgcc gcgacactcg tgcggctgtg cttggtcggg
     1261 cggcgacaac ccgatgaccg agttcgtgcc ttcgccatga tcgccgtcat tctcgccatg
     1321 ctttgtgccg ccgtcgcggt gggctattcg cgttcattca ttgggccggg aggggcccga
     1381 gtcggccgct acgtcacgac cacgtccccg ctgttctgtg ccctatatgt cgcctggttg
     1441 attcatggat cggctcgaac tcggcgtcgc gtccacgcgg ccctgttcgt ggtggtgatt
     1501 ctgacggtcc cgacgaatat caagcatggc ctgcgcgtcg gagaatggcg ccgcgcggtc
     1561 tacacccggc ttgagcgaga catgaaagct cattccactt tgtcggcggt cgtgaagaac
     1621 gcctggccgc atctttatcc cagtaaagag ttcattgaag tgagcttcaa gatgctcaag
     1681 gaagcccaag tgtgccgctt tggagatctt gtgtacgacc ggccggcgaa cgccccgaac
     1741 ggttcgagcg cgatccgata actggttgcg ccggcgcttg gagaggagac tttgccgtcg
     1801 ttgccttcta tgtccgcccc ggattgacgc ctcggacgat tgctcgaata ccggcgatcc
     1861 ggatcgagcc atcgtccagg agctcgggac ggaaactccg tatcgatggt cccgatcgca
     1921 ggagctctcg gacgtgatcc ttggtatccg acggcgagcc gcggtcgaac cattcgtcga
     1981 agtcgagcat gaacggttct tcgacgtagc gaatgtctgt caatccaacg cctacaaaaa
     2041 gatcaaccag ctcgccaccg gtgaggttcc tggtgtgagt acggtcgcgc gcgacctcga
     2101 tcttcccgtg gtgtgcggcc acttgcgaat cggggtcggt gatgtggtcg ttgacgacca
     2161 ggatcccacc cgaccggagc agctcgactt gtctcctcaa gaactgagcg ggatccaggg
     2221 tg
//
LOCUS       DATAQL010000385         7709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5381060, whole genome shotgun sequence.
ACCESSION   DATAQL010000385 DATAQL010000000
VERSION     DATAQL010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5381060"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VHS97_06635"
     CDS             <1..1072
                     /locus_tag="VHS97_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEX3447912.1"
                     /translation="AVRLGTGSTRSQFPPFTTDWDKKVEGFFRLVARAVSEPYPPSAV
                     ISGLTWAPASTIVRRARDSDNWPLTWADDDHLYTAYGDGTGFMPKVPEKLSLGLARIE
                     GGPDDFQAINIRSPTCERKGNGKNGLKASGMLMVEGVLYLWARNAGNAQLAWSSDHGL
                     TWTWADWKFTTSFGCPAFLNFGRDYAGARDHYVYVYSHDSESAYLAADRVVLARVPKD
                     RIRDRGAYEFFTGLGANGGAAWSADLNERGAAFSHQGKCYRTSVSYHAKMGRYLLCQA
                     GTDGPVQAGFGIYDAPEPWGPWTTVYQSEAWDVSPGESCCIPTKWMDDETQSIYLVFS
                     GNDSFAVRRATLRVAGKANDAR"
     gene            1171..2052
                     /locus_tag="VHS97_06640"
     CDS             1171..2052
                     /locus_tag="VHS97_06640"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3447913.1"
                     /translation="MTLANRVAIISGSGRGIGREMARLFSSEGARIVVADIDAESAGA
                     VAAEINAAGHVARATTVDVGDPAQVERLIRETVVHFGRLDVLVNNAGVGLNKPFLATT
                     PGEWEQQLRVNLTGTFLCAQAAAQVMVRQRAGTIVNVASISGQRGGQGRAAYGVSKAG
                     VILLTKVMAVELAPLGIRVNAIAPGPVDTDQSRGTHTQATRQAYFDRIPIRRYGERTE
                     IAAAALFLASDASSFVVGHVLNVDGGFQAAGLMFDPAEDATPWDEPDAERPHRSPAPA
                     ELRHGNGIPHATKGQAT"
     gene            2049..3290
                     /locus_tag="VHS97_06645"
     CDS             2049..3290
                     /locus_tag="VHS97_06645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447914.1"
                     /translation="MSDRHVVLSLLVALSMITYLDRVCIAIAGPRMQEELGISPEHWG
                     WVLGAFVFAYGVFEIPSGALGDRIGQRKVLTRIVVWWSVFTSLTGTVSSYLALLAVRL
                     LFGAGEAGAYPNMSGSIARWFPPTEQARAQGAVWAASRAGGVLAPWLVVPIMASLGWR
                     AAFWIFCALGLIWAAIWSAWYRDDPGPASNGHTSVGAGVPWGRLCRAPQLWLLMAMYW
                     CYVWGSMFYLTWFPTYLMYGRGLSEQEVGIYAASPFVLGVLGNLAGGHLSDRMARRYG
                     LGPGRRLVGTVSLTIAAALILVSALTPGKAASIVFLSLGFGAMDCMLPSAWAICLELG
                     NEHSGAVTGAMNSAGQAGGLACSVLFGYLVRASGSYDTPLFVIAAMVLASALLFAWID
                     PTRPLIPAEPGGLMAGEPACA"
     gene            3281..4096
                     /locus_tag="VHS97_06650"
     CDS             3281..4096
                     /locus_tag="VHS97_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3447915.1"
                     /translation="MRLGISSYTFVWGIGVPGYPLPPRPMTAHDLLERADALGVRVVQ
                     IADNLSLDRLDDADLAALRLSAEQKQIDLEVGTCGIDPANLNTHLRLAQYFKSPIVRV
                     VLDTAGHQPTPDEVVPALLSVLPAFERAGVCLAIENHDRFRSATLAEILDRLDSQCAG
                     ICLDTANSLGCLEDVETVLEVLGPRTVNLHIKDFQVFRPRHQKGFIIEGRPAGGGQLD
                     IPRLLARLRALGRDPNAILELWPPPEADSAAAAAKEEIWAAESVRYLRRLIPD"
     gene            4215..4312
                     /gene="ffs"
                     /locus_tag="VHS97_06655"
     ncRNA           4215..4312
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHS97_06655"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4499..6496
                     /gene="dnaX"
                     /locus_tag="VHS97_06660"
     CDS             4499..6496
                     /gene="dnaX"
                     /locus_tag="VHS97_06660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX3447916.1"
                     /translation="MAKNRSKKSDAAIADAGLAAPDTKPPAPSPPTAYTVVARRYRPQ
                     RFEDVVGQDHVVKALRNAIRLNRIAQAYLFCGTRGVGKTSMARIFAKCLNCVQGPTEQ
                     PCQNCDICQSIALGQDVDVIEIDGASNNGVEQVRELRQNAALRPSRARFKIYYIDEVH
                     MLSTGAFNALLKTLEEPPAHVKFLFATTEANKIPITVLSRCQRYDFAGITPDVIAKTL
                     GAICEQEHVEAEREALQIVARRASGSLRDAQSLLDRLLASGSPNLTVEVVNALLGTAS
                     DERLLAMLEALADHDSAAALRLLEESAGSGVQPAEILSGIIDFTRDALVMAVGAESML
                     ASVSPRQRPQLERIVERWTIDTILAASQIFAECRARMRGSLHGRLLVELALVRVARLE
                     DLTQLGTLVERLAALEAGTPGVRRPEGGVKSRIQALAESVSATAEPGPPPQVALPPSS
                     ADRPVTPSSVLRDSKTFPAAPPDAPRVVEDPRPSVPIALSVVPEVSETMALTEAEPTS
                     ATATATLLEPEPAPSSPREESSALDLAAARKVWPDVVKKVGAGLGWRLSQVEPTALAS
                     PDVLVIAAKPGYNAVADECGTADSLAKIAQAFQRLTHQSVTVKYESSSEDEGLAADAR
                     TPDARRADILASDPMIQKIIELFEARSLVFEYEEPDPNQSI"
     gene            6591..6956
                     /locus_tag="VHS97_06665"
     CDS             6591..6956
                     /locus_tag="VHS97_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX3447917.1"
                     /translation="MFNQLGMLADLMRNAGKLRESFEKAFESLGQVEVEGAAGGGSVT
                     VKVNGRLEVVSVRIDPKLLTESDHELLEDLVSAAVNAALLKAREAAAKSLGSMAGSLP
                     PGLFPDLGAGAKGPGSGGP"
     gene            6959..7570
                     /gene="recR"
                     /locus_tag="VHS97_06670"
     CDS             6959..7570
                     /gene="recR"
                     /locus_tag="VHS97_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX3447918.1"
                     /translation="MSGSGYAVAVDRLITALGQLPGIGAKSAERLAHHLLRCPPEEAL
                     ELAAAIHAAKSQIRHCEVCFHLTESDHPVCSICSDSRRDQATVCVVEQSRDLMALEKA
                     GAFHGVYHVLLGRLAPLSGIAADQLTIDALVARVRSGSIRELIMATNPNLEGDGTALL
                     IANRLSEAPVKITRLARGLASGGTLEFANRDMLADAFTGRQPL"
BASE COUNT         1358 a         2457 c         2485 g         1409 t
ORIGIN      
        1 ggccgtacgg ctgggcactg gttcgacccg ctcgcagttc ccccccttca ctaccgattg
       61 ggacaagaag gtcgaaggct ttttccggct cgtggcgcgt gcggtgtccg aaccttatcc
      121 ccctagcgct gtgatctccg gtctgacctg ggcccccgcg agcacgatcg tgcgcagggc
      181 ccgggacagc gacaactggc ctctgacttg ggccgatgat gaccatcttt acaccgccta
      241 cggcgacggc accggtttta tgcccaaggt gcccgaaaag ctgagcctgg ggctcgcccg
      301 gatcgaaggc ggccccgacg atttccaagc gatcaacatc cgttcgccca catgcgagcg
      361 gaagggcaac ggcaaaaacg gcttgaaggc gtccggcatg ctgatggtgg agggcgttct
      421 ttacctgtgg gcccgcaacg caggcaacgc gcagctcgct tggtcgagcg accacggctt
      481 gacctggacc tgggcggact ggaagttcac cacaagtttc ggctgccccg cgttcctcaa
      541 tttcggcagg gactatgctg gagccagaga tcattatgtt tacgtgtatt ctcacgattc
      601 ggaaagcgcg tatctggcgg ccgatcgcgt tgtgctggcc cgcgtcccga aggaccgaat
      661 ccgggaccga ggcgcctacg agttcttcac gggcctcggg gccaacggcg gcgcggcgtg
      721 gagcgcggat ctaaacgagc gcggggcggc ttttagccac cagggcaaat gctaccgcac
      781 cagtgtcagc taccatgcca aaatggggcg ctacctgctc tgccaggccg gaaccgacgg
      841 gcctgtacag gccggcttcg gcatctacga cgcgccagag ccctggggtc cgtggacgac
      901 cgtgtaccag agcgaggcgt gggatgtgtc tccgggcgag tcgtgctgca tcccgaccaa
      961 gtggatggac gatgagacgc agtctattta cctcgtgttc tccgggaacg attcctttgc
     1021 ggtgcggagg gcgaccttgc gagtcgcggg gaaagcaaat gatgcccggt agttacgcgg
     1081 acgcccgacc gcgcacgtcg cgcagcgcgg caagaggcgg tcgggctgag ctcgaatgcg
     1141 aacttgaatt gccaggggga ggatcgaacg atgacattag cgaaccgggt ggcaattatc
     1201 tcgggctcgg ggcgggggat cgggcgcgag atggcccggc tattttcctc ggagggagcc
     1261 aggatcgtcg tcgccgatat tgatgccgag agtgcgggcg ccgtagcggc cgagatcaat
     1321 gcggccgggc atgtcgcccg cgccaccacg gttgacgtcg gcgacccggc ccaggtcgaa
     1381 cgcttgatcc gggaaacagt cgtccatttc ggccggctcg acgtgttggt caacaacgcg
     1441 ggtgtcggac tgaacaagcc gttcctcgcg accaccccgg gcgagtggga gcagcaattg
     1501 cgggtgaacc tgaccgggac gtttctctgc gcccaggccg ctgcccaggt gatggtccgg
     1561 cagagggcgg ggacgatcgt caacgtggcg tcgatctcgg gtcagcgcgg gggccaaggc
     1621 cgtgcggctt acggggtctc caaagccggt gtgatccttc tgaccaaagt gatggccgtc
     1681 gagctcgcgc ccctgggcat ccgcgtcaac gccatcgcac cggggccggt cgacaccgac
     1741 cagagccggg ggacgcacac tcaggcgacc cgccaggcct actttgaccg catcccgatc
     1801 cgccggtacg gcgagcgcac agagatcgcc gccgcggcgt tgtttctggc gtcggacgca
     1861 tcgagcttcg tcgtcgggca cgtcctgaac gtcgacgggg gattccaggc cgccggactg
     1921 atgttcgacc ccgccgagga cgccaccccg tgggatgagc cagatgcgga gcggccgcat
     1981 cgctcgccgg cgcccgcaga gcttcgtcac ggcaacggca tccctcacgc gaccaaaggc
     2041 caagcgacat gagcgaccgc cacgtcgtcc tctccctgct cgtcgcgctg tcgatgatca
     2101 cctacttgga tcgggtgtgc atcgcgatcg cggggccgag aatgcaggaa gagctaggca
     2161 tttcgccgga acactgggga tgggtcctcg gggccttcgt attcgcctac ggtgtgttcg
     2221 agattccttc aggtgcactg ggcgaccgca ttggccagcg gaaggtcctg acgcggatcg
     2281 tcgtctggtg gtccgtgttc acctccttga cgggcacggt ctcgagctat ctcgcgctgc
     2341 tggcggtccg cctgctgttc ggtgcgggcg aggcaggtgc ttacccgaac atgtcgggaa
     2401 gcatcgcacg ctggttccct cccaccgagc aagcgcgggc gcagggggcg gtctgggccg
     2461 ccagccgggc cggcggcgtg ctcgccccgt ggctggtcgt cccgatcatg gcatcgctcg
     2521 gctggcgcgc cgctttctgg atcttctgcg cccttggtct catctgggcg gcgatctggt
     2581 cagcctggta tcgcgacgat cccggaccgg cgtcgaacgg gcacacgtcg gtgggtgccg
     2641 gcgttccctg gggccggctg tgccgcgctc cgcagctctg gttgctgatg gcaatgtatt
     2701 ggtgctatgt ctggggctcg atgttctatc tgacctggtt tccgacttat ttgatgtacg
     2761 gacgcgggct gagcgaacag gaagtcggga tctacgcggc ctcgccgttc gtcctcggag
     2821 tcctggggaa cctggccggc ggtcacctga gcgaccgcat ggcacggcgc tacggcctgg
     2881 ggccgggccg tcgattggtc ggcacggtca gcctgactat cgcggctgcc ctcatccttg
     2941 tttccgcctt gacgccaggc aaggcggcct cgatcgtctt cctgtcgctc ggcttcggcg
     3001 ccatggactg catgctcccg tcggcctggg ccatctgtct cgaactcggc aacgagcact
     3061 ccggcgccgt caccggagcg atgaactccg ccggccaggc cggtggcctt gcttgttcgg
     3121 tcctgttcgg ctacctcgtc cgggcgtccg gcagttacga cactccgctg ttcgtcatcg
     3181 cggccatggt tctggcgagc gccctgctct ttgcctggat cgatccgacg cgccccctga
     3241 tcccggccga gccgggtggc ctgatggcgg gagagccggc gtgcgcctag ggatcagctc
     3301 ctacacgttt gtctggggga tcggagtccc cggttatccc cttccgccca ggccgatgac
     3361 agcccacgac ctgctggagc gggccgatgc gttgggggtc cgcgtggtgc agatcgctga
     3421 caatctctcc ctcgatcgcc tggacgacgc cgatctggcc gccttgcgcc tgtccgccga
     3481 gcaaaagcaa atcgacttgg aagtcggaac gtgcggcatc gacccggcga acttaaacac
     3541 ccacctccgc ctggcgcaat acttcaaatc gccgatcgtc cgcgtggtac ttgacacggc
     3601 cggccaccag ccgacacccg acgaggtcgt gccggcgctc ctgagcgtcc tgcccgcgtt
     3661 cgaacgcgct ggcgtctgcc tcgccatcga gaaccacgat cgcttccgaa gtgccacgct
     3721 ggccgaaatc cttgaccggc tcgacagcca atgcgccggc atctgcctcg acacggccaa
     3781 ctcgctcggc tgcctggaag acgtggaaac cgtgctggag gtcctcgggc cgcggaccgt
     3841 caacttgcac atcaaggatt tccaagtctt ccgaccgcga caccagaagg gcttcatcat
     3901 cgagggccgg ccggccggag ggggacaact cgacattcct cggctgctgg cccggttgcg
     3961 ggcgctgggg cgggacccca acgcgatcct ggaactttgg ccgccccccg aagccgacag
     4021 cgccgcagcc gcggccaagg aagagatctg ggccgctgag agcgtccgct acctgcgccg
     4081 gctgatcccg gactgaccgg gaaataaaga tccgattggt cacatttcgg tcttgccctt
     4141 gaaccgagtc aatggatgtc tgacaatgta tggttgaccg gctcctgtcg tcacgacggg
     4201 ggtcagggtg agccgggggg gacccgggca atgggggtat gtgaacctgg tcagggcggg
     4261 aacgcagcag ccataagcaa caccttcatg tgcctgggag aacctccccg gctctcccag
     4321 atccccgcgt ggaagccgtg ccgcgggtca agctaggtcg gccgtcccgc tcgtcgtggc
     4381 gcggagtgat cgcaggagaa agcgacttta cggaaagcca agccggatca ggtgagtttg
     4441 catcctggac gactcacggt tcggcacgat ggagcgggcc ttactggccg tgtgacaggt
     4501 ggcgaaaaat cgatcgaaga agtcagacgc ggcaattgcc gacgcgggcc tggcagcgcc
     4561 cgatacgaaa ccgccagcgc ccagcccgcc cactgcgtac accgttgtcg cccggcgcta
     4621 tcggccgcaa cggtttgaag atgttgtagg ccaggaccat gtcgtcaagg cgctgcgcaa
     4681 tgcgatcagg ctcaaccgaa tcgcccaggc ctatctgttt tgcggcacgc gcggggtcgg
     4741 caaaacctcg atggcacgga tcttcgccaa atgcctaaat tgcgtgcagg ggccgaccga
     4801 gcaaccgtgc cagaactgcg acatctgcca atcaatcgcg ctgggccagg acgtcgacgt
     4861 catcgaaatc gacggtgcca gcaataatgg cgtcgagcag gtgcgcgaac tgcggcaaaa
     4921 cgccgcactt cggcccagcc gggcccggtt caagatttat tacatcgacg aagtacatat
     4981 gctctcgacc ggggcgttca atgcgcttct aaaaacgctc gaagaaccac cggctcatgt
     5041 gaagttctta ttcgcaacca ccgaggcaaa caagattcca atcacggtgc tctcacgctg
     5101 ccagcgctac gatttcgccg gcatcacgcc cgacgtcatc gccaaaacgc taggcgctat
     5161 ttgcgaacag gagcacgtcg aggcggagcg agaggctttg cagatcgtcg ctcgtcgcgc
     5221 cagcggctcg ctgcgcgatg cgcagtcgct cttggatcgg cttttagcct caggaagtcc
     5281 caacctgacg gtggaggtag tcaatgcctt gctaggaacg gccagtgacg agcggttgct
     5341 ggcgatgctc gaggccctgg cggatcacga ttccgcggcc gcactgcggt tgctcgaaga
     5401 gagcgccggc tcgggggtcc agcccgcgga gatcctctcc gggattatcg atttcacgcg
     5461 agacgcgctg gtcatggccg tcggcgccga atcgatgctg gcctcggtct cgccccggca
     5521 acgaccacag ctcgaacgta tcgtcgagcg ctggaccatt gacaccatac tggcggcatc
     5581 acagattttt gcggagtgcc gcgcccggat gcgcggcagt ctgcacggca ggctgctggt
     5641 ggagctcgcg ctcgtgcgtg tggcgcggct cgaagacctg acccagttag gtacgctcgt
     5701 cgagcggctc gccgcgctcg aggcgggcac gcctggagtc agaaggcccg aaggaggcgt
     5761 caagtcaaga atccaagcct tggcggaaag tgtgtccgcg acggccgaac cgggccctcc
     5821 gccacaagtg gctttgcccc cttcgtctgc cgatcgaccg gttacaccct cgtccgtgtt
     5881 acgcgactcc aagacatttc ccgccgcgcc gccagacgcg ccccgtgtcg tcgaagatcc
     5941 gcgaccgtca gtccccatcg ctctctcggt cgttccggag gtatcagaaa cgatggcgct
     6001 caccgaggcc gagcccacct cggcgactgc gactgcgacg ttgctcgagc ccgaaccggc
     6061 tcccagttcg cctcgggagg aatcgtcggc gctggatctg gcggccgcgc gcaaagtttg
     6121 gcccgacgtg gtcaagaaag tcggtgccgg actgggatgg cgcctgagcc aggttgagcc
     6181 gaccgctctt gccagcccgg acgttctggt catcgccgcc aagccaggat acaatgccgt
     6241 tgccgacgaa tgtgggactg cggactccct ggcgaaaatc gcccaggctt tccagcgact
     6301 gacccaccag tcggtaaccg tcaagtacga aagctcttcc gaagacgaag gcttggcggc
     6361 cgatgcccgg acacccgacg ctcggcgggc cgacatcctg gcatccgacc ccatgattca
     6421 gaaaatcatc gagctgttcg aggcccgttc actcgtattc gaatacgaag aaccagatcc
     6481 taatcagtcg atttgagcaa tcccgagcca acggaccgag tgacagccga ggtcgactcg
     6541 tcgggagttg tgagtcaaat cggtaaaacc tccaggtttt gaaggaaaac gtgttcaatc
     6601 aactgggaat gctcgcggac ctgatgcgaa acgccggcaa gttgcgcgag tcgttcgaaa
     6661 aggcgtttga gtcgctgggc caggtcgagg tcgagggggc tgccggcggt ggttcggtta
     6721 ccgtcaaggt caacggtcgg ctcgaggtag tgtcggtgcg aatcgatccc aagttgctca
     6781 ccgaatccga tcacgaactt ctggaagacc tggtatccgc ggcggtaaac gccgcacttt
     6841 tgaaagccag agaggccgcg gccaaatcgc tgggctcgat ggcaggcagc ttaccgccgg
     6901 gactcttccc cgacctggga gcgggggcaa agggcccggg cagcggggga ccttgacgat
     6961 gtctgggtcc ggatacgcgg tggccgtgga tcgattgatc accgccctcg ggcaattgcc
     7021 gggaatcggg gccaaatccg ccgagcgtct cgctcaccat ttgctcagat gccctccgga
     7081 agaagcactc gagctggccg cggcgatcca cgccgccaaa tcccaaatcc ggcactgcga
     7141 ggtctgtttc catttgaccg agtctgacca cccggtctgc tcgatctgct ccgattcgcg
     7201 ccgtgatcag gcgacggtgt gcgtcgtcga gcaatcacgt gacttgatgg ctctggaaaa
     7261 agcgggcgca ttccacggag tttatcacgt ccttttgggc cgcctggcgc ctctttccgg
     7321 cattgcggcg gatcagctaa ccatcgacgc tcttgtcgcc cgcgtccgct caggatcgat
     7381 ccgtgagctg atcatggcca ctaatcccaa cctcgaagga gacggaaccg cacttctgat
     7441 tgccaatcgc ctgagcgaag cccccgtgaa gatcacgcgg ctcgcgcgtg gtctggcatc
     7501 cggcggtacc ctcgaattcg ccaaccgcga catgctagct gacgccttca ccggccggca
     7561 gcccctctag cttctttttc aagtccacct cttgtccccg cccggtctgt ggcataatag
     7621 ttgctagaag gattcgctgt cctgggctgg tcgctgttat tcatcgcgtc tgccactgcc
     7681 ttgttataac ggaagcagta aatcatgcg
//
LOCUS       DATAQL010000386         4357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3854799, whole genome shotgun sequence.
ACCESSION   DATAQL010000386 DATAQL010000000
VERSION     DATAQL010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3854799"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /gene="treZ"
                     /locus_tag="VHS97_06675"
     CDS             <1..1839
                     /gene="treZ"
                     /locus_tag="VHS97_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX3447919.1"
                     /translation="PVRRTGAIGSEGGSILWRVWAPFASAVSLVLIDGDQRREIAMQS
                     EGRGLHRAILRGVPEGQRYAYRLDDGPDRQDPCSLWQPDGVDGFSAVVIPDRFSWTDP
                     DWTGVPQAELVFYELHVGTFTAEGTFDAIIPRLGDLRELGITAIEIMPVAQFPGSRNW
                     GYDGVLPYAAQNTYGGPHGLQRLIDACHACGLAVYLDVVYNHFGPESNYLGEFGPYFT
                     DRYKTPWGAAINYDLAGCDAVRDFVLDNVRMWLEEFHVDGLRLDAVHAIFDMGARHIL
                     RAIKEAANKAGASRGWPAIVTAECDLNDPRLLYSVERGGYELDAQWADDFHHATHAFL
                     TDERHGYYIDFGDASQLAQALELPFLYNWDYSAFRDRKHGARPEGLSGNRFIVCLQNH
                     DQVGNRASGDRLNSLLKSRAAQRLAASYLLLSPYLPLLFMGEEYGEENPFPFFCSFRG
                     AELQQAVQEGRRREHSAHHQQALVPDPCAEATFTSARLSWSWPEGSERLGLRRLYRDL
                     LTARREWPALKDLVCRSARLIRDHERGMLLKAHFGASPERSVHALFNLENRPIALAAD
                     SRTGQNILFSSESESYRGGRTQFRRIHELLAHECVVFGPSSWRSFI"
     gene            1955..4171
                     /gene="glgX"
                     /locus_tag="VHS97_06680"
     CDS             1955..4171
                     /gene="glgX"
                     /locus_tag="VHS97_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX3447920.1"
                     /translation="MRLWPGHPYPLGATWDGAGVNFAIHAENATKVELCLFDSADAEN
                     EAERITLKEYTDKVWHVYLPEVLSGQLYGYRFHGPFDPSKGHRFNPNKIVLDPYAKLI
                     GRDARWADELFGYRFDDPEADLSFDDRDSAPYAPLATVIDTAFTWGDDRPPRTPWHKT
                     VIYELHVKGFTQNNPNVPDKLRGSYAGLASESALKHLTDLGVTAVELLPVHYHLDDRH
                     LTEKGLKNYWGYNTLGFLAPEPDYCCTSIPQDNVQQFKMMVRAMHAAGLEVILDVVYN
                     HTAEGNQMGPTLSMRGVDNAVYYRLDSDPRYYMDFTGTGNTLNMTNPHVLQLIMDSLR
                     YWVADMHVDGFRFDLASTLARELFDVDRLGAFFDIIHQDPVLSQVKLIAEPWDVGPGG
                     YQVGNFPVLWTEWNGKYRDCVRRFWKGDGGTTSELATRLTGSSDLYQDDGRKPYASIN
                     FVTCHDGFTLDDLVGYNEKHNEANGEENRDGANDNNSWNCGAEGPTDDPAINELRERQ
                     KRNLMASLMLSVGVPMMLGGDELSHSQNGNNNTYCQDNELTWLDWDLDERKQQFMEFT
                     KKVLFIRMTQPVFQRRKFFLGRAIRGSDVKDISFFNPSGDEMSDGDWNAGFAKCLGFR
                     LAGDLINDDSERGDPIIGETLLVLLNGHWEPIPFTLPPTLHGHIWERLLDTAETNPTA
                     AAFEASGEYPLRERSLVVLVTRKPEDKGQIFTSTEARSALTGAKESGRTPPGPSPI"
BASE COUNT          889 a         1283 c         1280 g          905 t
ORIGIN      
        1 ccggtgcgcc gaacgggagc catcgggtcc gagggtgggt cgattctttg gcgggtgtgg
       61 gctccgtttg caagcgcggt gagcctcgtc ctgatcgacg gagaccagcg gcgagagatc
      121 gccatgcaaa gtgagggtcg aggattgcat cgtgcaatcc tgaggggcgt acccgaaggt
      181 caacgctatg cctatcggtt ggacgacggt cccgatcgac aggatccttg ctcgctttgg
      241 cagccagacg gagttgatgg attctccgcc gtggtaatac ccgaccgatt ttcatggacc
      301 gacccggact ggacgggcgt gccgcaggcg gagctggtct tttacgagct gcacgtcggt
      361 acctttacgg cagaagggac cttcgacgcg atcattccca ggctcgggga cctacgcgag
      421 ctcggtatta cggccatcga gatcatgccg gtcgcccagt ttccgggatc acgcaattgg
      481 ggatacgacg gtgtgctccc ctatgcggca cagaacacat acggcgggcc tcacgggctg
      541 caaaggctaa tcgacgcctg tcatgcctgc ggactggccg tttatcttga cgtagtttac
      601 aaccacttcg gtcccgaatc gaactatctc ggcgagtttg gcccttactt cacggatcgt
      661 tacaagactc cttggggcgc cgccatcaac tatgacctag ccggctgcga cgccgtgcga
      721 gactttgtgc tcgacaatgt gcggatgtgg ctcgaagagt tccacgttga cggcttacgt
      781 ctcgatgcgg tgcacgcgat cttcgatatg ggggctcgtc atatcctgcg agcaatcaaa
      841 gaggcggcga ataaggcggg tgctagtaga ggatggccgg cgattgtgac cgccgaatgt
      901 gatctcaatg atccccggct gctctattcc gtcgagcgcg gcgggtatga gctggacgcg
      961 cagtgggcgg acgacttcca ccatgccacc cacgcgtttt tgacagatga gcgtcacggg
     1021 tactacattg actttggcga cgcgtctcaa cttgcgcagg cattggaact tccgttctta
     1081 tataactggg attatagcgc ctttcgcgat cggaaacacg gggctcgtcc cgaggggctc
     1141 tcgggcaatc gctttattgt ctgcctccaa aatcatgatc aggtcggcaa tcgcgccagc
     1201 ggcgatcggc tcaattcgtt gctcaagtca cgagccgcac agcggctcgc cgccagctat
     1261 ctcttgcttt caccgtattt gccgcttctg ttcatggggg aagaatatgg cgaagaaaat
     1321 ccgttcccgt ttttctgctc gtttcgcggg gcggagctgc aacaggcggt gcaagagggc
     1381 cgccgacggg aacacagtgc tcatcaccag caggctctgg ttccggaccc ctgcgcggaa
     1441 gccacgttca catcggctcg cctgagctgg tcatggccgg aaggttcaga gcgtctcggt
     1501 ttgcgccgcc tctaccgcga cctcctgacc gcgcgtcggg agtggcccgc gcttaaggat
     1561 cttgtttgcc ggtcggctcg attgatccgg gatcacgaaa gaggcatgct gctcaaggcc
     1621 catttcggtg caagccccga gcgatcggtg catgctttat ttaacctcga aaaccgccca
     1681 atcgcgctgg cggcagactc ccgcacggga cagaacatcc tctttagttc cgagtctgag
     1741 tcttaccgtg gcggccgcac acaattccgt cgtattcacg agctcttggc ccacgagtgc
     1801 gtcgtcttcg gtccgtcatc atggaggtca ttcatttgat ctcggcgaaa atcgcatgac
     1861 atgcctggga tctaccaatt gagctttggt gtttgtaagt ttgatagtat gtcatgcaaa
     1921 tctgattaga aatcgtcacg aacggggagc ctccatgaga ttatggccgg gacatccgta
     1981 tccactgggt gccacatggg acggtgccgg tgtcaatttc gccattcatg cggaaaacgc
     2041 caccaaagtt gagctctgtc tgtttgattc cgccgacgcg gaaaacgagg cggaacggat
     2101 cacgctcaag gagtacacgg acaaggtctg gcacgtgtat ctgccggaag tcctgtcggg
     2161 ccagctttat gggtaccggt ttcatggtcc gttcgatccg tccaagggcc accggttcaa
     2221 cccaaataag atcgtgctgg acccgtatgc caagctcatt ggccgcgacg ctcgctgggc
     2281 cgacgagctc tttggctatc ggtttgacga tcccgaagcc gacctgtcgt ttgacgaccg
     2341 cgacagcgca ccctatgctc cgctcgcgac ggtgatcgac acggcattca cgtggggcga
     2401 tgatcggccc ccacgcacac cctggcacaa gaccgtgatt tacgagctgc acgtgaaggg
     2461 attcactcag aacaacccca acgtgcccga caagctgcgc ggcagctacg ccggcttggc
     2521 gtccgaatcg gctttaaagc acttgaccga cctgggagtg acggccgtcg agctcttgcc
     2581 cgtgcactac cacttggacg atcgccacct gaccgagaaa ggactgaaaa actactgggg
     2641 ttacaacacg ctgggtttcc tggctcccga gccggactac tgttgcacga gcatcccgca
     2701 agacaacgtg cagcaattca agatgatggt ccgtgcgatg cacgcggcgg ggctcgaggt
     2761 gatcctcgac gtcgtctata atcacaccgc cgagggcaac cagatggggc ccacgctttc
     2821 gatgcgcggg gtcgacaatg cggtgtacta tcgcctggac tcggatcccc gttactacat
     2881 ggacttcacg gggacgggca acacgctcaa catgaccaac ccccacgtat tgcagctcat
     2941 catggatagc ctgcgctact gggtggccga tatgcacgtg gacggttttc gatttgacct
     3001 tgccagcaca ctggcgcgcg agcttttcga tgtcgatcga ttaggcgctt ttttcgacat
     3061 catccatcaa gatccggttc tctcgcaggt caagctgatc gccgagccct gggacgtcgg
     3121 tccggggggc tatcaggttg gcaattttcc ggtcctgtgg accgagtgga atggcaagta
     3181 tcgcgactgt gtgcgccgct tttggaaagg cgacggaggc actacgtctg aactggccac
     3241 gcgcctgacc ggcagcagtg acctctacca ggacgatggt cgcaagccgt acgccagcat
     3301 caatttcgtc acgtgccatg acggcttcac gctggacgat ctggtcggct acaacgagaa
     3361 gcacaacgaa gccaacggcg aggaaaaccg cgacggcgcc aacgacaaca atagctggaa
     3421 ttgcggcgcc gaggggccga cggacgaccc cgcgatcaat gagcttcgcg agcggcaaaa
     3481 gcgcaatctg atggccagcc tgatgctttc ggtaggcgta ccgatgatgc tgggcggtga
     3541 cgagctttcc cacagccaga acgggaacaa caacacgtac tgccaggaca acgagctcac
     3601 ctggctcgac tgggatctcg acgaacgcaa gcagcaattc atggagttca ccaagaaagt
     3661 tctcttcatc cggatgaccc agccggtctt tcagcgccgc aagtttttcc tgggaagagc
     3721 catccgcggc tcggatgtca aggacatctc gttcttcaat ccatctgggg atgaaatgtc
     3781 cgatggcgac tggaatgccg gtttcgccaa atgcctcgga ttccgcttgg ccggcgacct
     3841 gatcaacgat gacagcgagc gcggcgatcc aattatcggc gagactctcc tggtcctctt
     3901 gaacggtcac tgggaaccaa tccccttcac cctcccgccg acgttgcatg ggcatatctg
     3961 ggagcgcctt ttggataccg cggagacaaa cccaaccgcg gcggcctttg aagcttccgg
     4021 cgaatatcct ttgcgagaac gttcactggt tgtcctggtc actcgcaaac ccgaagacaa
     4081 gggacagatc ttcacgtcca ccgaggccag atctgccctt actggggcga aagagagcgg
     4141 cagaacaccg ccaggccctt ctccgatctg agcttggcgc gcagcgatct tgagcacgcg
     4201 agttaaccat gagcccgatc gaattcgccg acaagctgct ctcagaagcc gtcggtgcgg
     4261 cagccgcgcg caggccgtta cctgaatcga cctaccgctt gcagtttcac gcgggattta
     4321 ccttccgcga cgcgctcgag atcacgccgt acttgag
//
LOCUS       DATAQL010000387         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4769768, whole genome shotgun sequence.
ACCESSION   DATAQL010000387 DATAQL010000000
VERSION     DATAQL010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4769768"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..2491
                     /gene="pruA"
                     /locus_tag="VHS97_06685"
     CDS             143..2491
                     /gene="pruA"
                     /locus_tag="VHS97_06685"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX3447921.1"
                     /translation="MLSAARELGAYIHVDMEQYTYRALSYELFCRVLAEPKFRDWPDV
                     GIVVQAYHGDAENELQMLREWVERRGTPITIRLVKGAYWDYEVSTARRLGWPEPVYLQ
                     KWQSDASFERCGRYLIEHHEQLRPAFGSHNVRSLAHAIATAEARGLPRNAYELQTLIG
                     MGDLIERTLVDRGHRVRVYTPYVAILPGMAYLVRRLLENTSNESFLKASFAEKAQIDD
                     LLRDPEEIGSMLTRTRRPQTASPKTPSASGATDLPAFRNEPPTDYARAENREAMRQAL
                     EEVRPQLGRRYPLLIGGNEVDTGTRVLDSVDHGHSSRVVGRVALAGVEHAAAAVKAAR
                     AAFPQWAATPAPDRAAILLKAATIMRSRRFELAAWEVYECGKPWAEADADVAEAIDFC
                     EFYAREMIRLAEPRHRDASGETNFTEHLARGVAVVIPPWNFPLAIPTGMTVAALVAGN
                     AVVLKAAEQSPVITWQLARILGEAGLPPGVLNFLPGLGEEVGQALVNHPDIDIVAFTG
                     SREVGLLINRQAAVVKPGQDHLKRVIAEMGGKNAILIDDDADLDEAVVGVIHSAFGYS
                     GQKCSACSRVIVLQGIYDAFLARLVEAARAVKVGPASDPETMVGPVIDADARRRILEY
                     QRIAATEGRVVFHSDAGPHADQGYFVGPIIVADVQRDARVAQEEIFGPVLAVLKANDL
                     DDGLCIANGTQYALTGGLYSRSPANIDRVRREFLVGNLYINRGITGALVDRQPFGGFK
                     LSGIGTKAGGPDYLLEFLLTRCVTENTMRRGFAPEDNDADPK"
     gene            complement(2598..>3486)
                     /locus_tag="VHS97_06690"
     CDS             complement(2598..>3486)
                     /locus_tag="VHS97_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447922.1"
                     /translation="VAAALVAETFPSHARAAASGIFHASSILGSALASLTGMVFVGPG
                     TWRWGFVMGLAPALLVLWIRLSMREPERWVAARRAEVSPAAPALGNLRELFGRQPWRS
                     RALLGLALATVGLATYWGIFAWSPELVGVILGDSASPAERQQLGSRAYLLMNFTGGLL
                     GLLAFAPLASWRGRRFAFAVYHLGAAIVAPVAFLGASTYVQALWLLPVMAFFVLGMHA
                     GYAIYFPELFPTRLRATGSSVCFNLGRVLGAAILIVRGTLGSALGLRWAVVAMSGLFW
                     IGLLVLVFAPETGGRELPE"
BASE COUNT          675 a         1125 c         1097 g          589 t
ORIGIN      
        1 cgagattccc tcgatcgacc gcgaccaaaa cgggccgatg ccgcgggtga acctttcgct
       61 caagctgtcg agcttgaccg cccagttcta gccgatccac cccgaagcaa ccatcgatcg
      121 ggtcagccgg cggctacggc ctatcttgag cgctgcacgg gagctgggag cttatatcca
      181 cgtcgacatg gagcagtata cgtaccgggc gctctcgtac gaattgtttt gccgcgtgct
      241 cgccgagccc aaatttcgcg actggccgga tgtcgggatt gtcgtgcaag cgtatcacgg
      301 cgatgccgag aacgagctgc agatgctgag agaatgggtc gagcgccgcg gcacaccaat
      361 cacgatccgg ctcgtcaagg gagcgtactg ggactacgag gtctcgaccg cgcggcggct
      421 gggctggcct gagccggtct acctgcaaaa atggcaaagt gacgcttctt tcgagcgctg
      481 cggccgatat ttgatcgagc accacgagca gttgcgccct gcgttcggca gccacaacgt
      541 ccgaagcctt gcacacgcga tcgccaccgc tgaagcccgc gggctgccgc gcaatgccta
      601 cgagctccaa accttgatcg gcatgggtga cttgatcgag cgtaccctgg tcgatcgcgg
      661 ccaccgcgtt cgggtctata cgccttatgt cgccatactg cccggcatgg cctatctggt
      721 ccgccgctta ctcgaaaata cgtcgaacga atcgttcttg aaggcgagct tcgccgaaaa
      781 agctcagatc gacgatctgt tgcgcgaccc cgaggagatc ggatccatgt tgacgcggac
      841 gcgacgcccc cagaccgcat ctccgaaaac gccatcggca tccggcgcga ccgacctgcc
      901 cgcctttcgc aatgagccgc caaccgatta tgcgagagct gagaaccgcg aggcgatgcg
      961 tcaggcgctt gaagaggtcc gcccccagct cggccgccgc tatccgctct tgatcggcgg
     1021 caacgaggtc gacacgggca cgcgggtcct tgattcggtg gatcacggcc atagctcgcg
     1081 ggtcgtgggc cgggtggcgc tggctggagt cgagcacgcc gcggccgcgg tgaaggcggc
     1141 ccgggccgct ttcccgcaat gggcagccac cccggcgccc gatcgggccg ccatactgtt
     1201 gaaggcggcc acgatcatgc ggagccgccg cttcgagctg gccgcgtggg aggtttatga
     1261 atgcggaaag ccctgggccg aggcggatgc cgacgttgcc gaggcgatcg atttttgcga
     1321 gttctatgcc cgcgagatga tccgactagc cgagccgcgg caccgggacg cctctggcga
     1381 gacgaatttc accgagcacc tcgcgcgtgg tgtagccgtg gtgattcctc cctggaattt
     1441 ccccctggcc atccccaccg gcatgaccgt ggccgccctg gtcgcgggca acgctgtggt
     1501 cttgaaggcc gcagagcaat caccggtcat cacgtggcaa ctcgcccgta ttcttggcga
     1561 agccgggctg ccgccaggcg tgctcaactt tcttcctggc ttgggagagg aagtcggaca
     1621 ggcgctcgtc aatcatccgg acattgacat cgtcgcgttc accggttcgc gcgaggtcgg
     1681 tcttttgatc aatcgccagg ccgccgtggt caagccgggc caggatcatt tgaagcgagt
     1741 catcgccgag atgggaggca agaacgcaat cttgatcgac gacgatgcgg atctcgacga
     1801 ggcggttgtg ggagtgatcc acagcgcctt cggttattcg ggtcaaaagt gctcggcgtg
     1861 ttcgcgcgtg attgttctcc aagggattta cgacgccttt ctcgcccgac tagtcgaagc
     1921 agctcgagcg gtcaaagtgg gtccggccag cgatcccgag accatggtcg gcccggtgat
     1981 cgacgccgac gcccgccggc gcatcctcga gtatcagcga atcgccgcca ccgagggccg
     2041 cgtcgtattc cattctgatg caggtccgca tgccgaccag ggctacttcg tcgggccaat
     2101 aatcgtcgcc gatgtccaac gtgatgctcg cgtggcccag gaggagatct tcggtccggt
     2161 gttggccgtc ctcaaggcaa acgatctcga tgacggcttg tgtatcgcca acggcactca
     2221 gtacgccctt actggaggtc tgtactcacg cagcccggcc aatatcgaca gagttcggcg
     2281 cgagttcctg gtcggcaacc tttacatcaa tcgaggtatc accggcgcac tcgtcgatcg
     2341 ccagcccttt ggtggtttca agctttcggg catcgggacc aaggccggcg gtcctgatta
     2401 tttgctagag tttctcttga cccgttgtgt cacggaaaac acgatgcgcc gggggttcgc
     2461 gcccgaggat aacgacgccg acccaaagta ggcccttgag gcccgcgtat cgatcgtttc
     2521 gcggatgctc tcatttccgc gcctggatgg aacgccgaat gaggctgatt cgtcgacagc
     2581 gtcagcaaag tttgccctca ctccggcagt tctcgccccc ccgtttccgg agcaaacacc
     2641 agaaccaaga gcccgatcca gaacaggccc gacatggcga cgaccgccca gcgcaatccc
     2701 agcgccgagc ccagtgtgcc acgcacgatc agaatcgcgg cccccagcac gcggcccagg
     2761 ttgaagcaca cgctcgagcc cgtggcgcgg agccgcgtgg gaaacaactc cgggaaatag
     2821 atcgcgtacc cggcgtgcat ccctagaacg aagaaggcca tcaccggcag gagccagaga
     2881 gcctggacgt aggtgcttgc gcccaggaac gcgaccggcg ccacaatcgc ggctcccaaa
     2941 tgatagaccg cgaatgcgaa ccggcgaccg cgccacgacg ccaggggagc gaaagcgagt
     3001 agaccgagaa gaccaccggt aaagttcatc aagagatagg cgcggctccc caattgctgg
     3061 cgctcggccg gcgaggcgct gtcgcccagg atcacgccga ctagttcggg gctccaggcg
     3121 aatatccccc aatacgttgc caacccgaca gtggcgagag ccagccccaa gagcgcccgc
     3181 gaccgccacg gctggcggcc gaagagctcg cgcagattgc ccagggcggg agccgccggc
     3241 gaaacctcgg ctcgacgggc ggcgacccat cgctcaggtt cgcgcatgga gaggcgtatc
     3301 cagagcacca aaagtgctgg ggccaatccc atgacgaatc cccaccgcca cgtgccgggg
     3361 ccgacgaaga ccatgccggt cagcgacgca agtgcgctgc ccagaatgct cgacgcgtgg
     3421 aagatccccg aagcggcggc acgggcgtgg gagggaaagg tctcggccac caaggccgcg
     3481 gcgacc
//
LOCUS       DATAQL010000388         6704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1564420, whole genome shotgun sequence.
ACCESSION   DATAQL010000388 DATAQL010000000
VERSION     DATAQL010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564420"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1001
                     /locus_tag="VHS97_06695"
     CDS             105..1001
                     /locus_tag="VHS97_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3447923.1"
                     /translation="MIVTKGLTKVYKRVTALEDLCLQVGRGEVYGLLGPNGSGKTTTI
                     RLLLGLLRPTAGWATVAGFDSWRSSLDVRRLVSYLPGELRMYGSLTGLGTLRLLCDLR
                     EGAGLDRAVAIAERVMKLNLKRKIRTYSTGMKQKLALAQVFSDPVDTLILDEPTSALD
                     PSARSIVIGLVQEARSGGQTVIFSGHVLAEVEQVADRVAIMRKGRLMHVEDMHVRRSV
                     RMLLIRFEGGIEPQFPPELELSVRERNGETLLLEHRGDLSSLLRWLASEAVVDIAIGT
                     EDLRALYDRFHGLNVPDEEAPV"
     gene            998..1837
                     /locus_tag="VHS97_06700"
     CDS             998..1837
                     /locus_tag="VHS97_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3447924.1"
                     /translation="MRPIWALLRKQIVESRWTLGLSAAALFGLGWLFVYVTSLNEAEI
                     LRLLESDSEGGGRIQWMRNLGVADQPTSVAIMMAFWNHPFIVLLFSIWAIGRGSAAVS
                     AEIERGTLDLILSRPVSRSAYLASQVFVAIVGLLILGAALLAGASIAVGYNALHEPPI
                     SWTLFKPALNLCALGLPIYGYTLLASALDHVRWRPTTIGSILTLGGFIAYVISTIPVL
                     KDMSWKPWLERISIFKAYNPVELVTTGETLQFNLALLSGIGVSCIALAFVAFAVRDLP
                     TNG"
     gene            1850..3001
                     /locus_tag="VHS97_06705"
     CDS             1850..3001
                     /locus_tag="VHS97_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03364 family FAD-dependent oxidoreductase"
                     /protein_id="HEX3447925.1"
                     /translation="MIGQHRFDDAVVGAGILGLAHAYHLARRGRRVIVFERTPRAMGA
                     SVRNFGMLWPIGQPPGRLRQMAMRSLQHWLQVLASSSLWHEQTGSLHLAYRDDEAQVL
                     REFGRELSAQGEAVDLLIPDQVQERTRAVKRDGLQLALWSPHEVCIDPREVIAGLPPW
                     LAIRFGVVFEFGRTITAVETRRVISGQGSWSAERVWICSGDELRLLFADQFEDCGLLR
                     CKLQMMRSQPYGQERRIGPMLASGLTLRHYSAFQNCPTLPALRRRISEESPWFDRYGI
                     HVLVSQNGRGELTIGDSHEYGAAIEPFDKTEVDEYILDYLKTFVDVPGLRIASRWHGT
                     YAKHPTLPYVLVHPLPGVTAITGVGGAGMTLSFGLAEQVVARELGEPEA"
     gene            3023..3715
                     /locus_tag="VHS97_06710"
     CDS             3023..3715
                     /locus_tag="VHS97_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX3447926.1"
                     /translation="MKDIALVVFDIAGTTVNDDDSVNRCLRGSLSAAGLLVTAAQVNT
                     VMGLPKPDAIAILIGQSPLRDELRGRVDLIHRDFVARSIEFFGSDPSVHEVPSTTRVF
                     ETLKRSGILVALDTGFNRSITQVILDRLDWSGSRLIDATICSDEVRRGRPHPDMIETL
                     MSRLGIADPKRVAKVGDTPSDLQEGHAAGCRLVVGVTAGTHTRLQLESCPHHYLIETI
                     ADFPGLLGLEIG"
     gene            complement(3863..4963)
                     /locus_tag="VHS97_06715"
     CDS             complement(3863..4963)
                     /locus_tag="VHS97_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447927.1"
                     /translation="MLRPIPVALGLALVLWLSEGTVHAQGWGWGGWGGWGATPGGDYA
                     RGLGYFNMGAGVYNLDTAQARAINTDTFMRWNQYLYEAHLEATRQYVAKRDGDAAKNK
                     AVYNELLKNLREHPTARDVENGDALNAALDQLSDPRISSSSLKIATSPVDAQIIKDIP
                     FRNASEAVTIVLSQVKAASKWPSALNNDRFASDKKNFEEIVDKVRAEDEEGDISAETL
                     TRAKNLVSSLRAKIESTPFDNLAQSQEASKFIKTLAGLVRMLEKPDTREAFDQLRMVK
                     TSSLGNLLAFMHIYNLRFGAATTPRQRIIYEQLFPLLDEVRDRVVKESNVEDTATARA
                     QPAHVGEFFNKLDLDSLQGKDKKNAPPQPNNP"
     gene            complement(5179..5625)
                     /locus_tag="VHS97_06720"
     CDS             complement(5179..5625)
                     /locus_tag="VHS97_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3447928.1"
                     /translation="MSFRLVPMIPGTAPQVALHRPVLLIGRHLECDVRIDSPKISRRH
                     CCLAMAYDRVLIRDLGSRNGVRVNGRLVEEIRLHPGDELAIGPVLFRLEAESEEPRNS
                     GAMISRPSQPPKAARPVGAPANVQAAALPPRARSDSEIDLVPLDDF"
     gene            complement(5601..6191)
                     /locus_tag="VHS97_06725"
     CDS             complement(5601..6191)
                     /locus_tag="VHS97_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3447929.1"
                     /translation="MPARLVALDEGPDIILDRAMVVVGRHPSCDTRLDSLRVSRHHCC
                     MTHESGDVMVRDLGSTNGIRINGQRVEIGRVRPGDELSIAHIRYRLENGQGHEQTIAE
                     AGGLGKMARSADDEDRGGSLDARVADAVPLGNGGENAFAAAVRKLLPNGVADKCRIQV
                     IVKMAGDSGSSDGEPAPLVPNHASENAAECHSDSSP"
BASE COUNT         1301 a         1911 c         1998 g         1494 t
ORIGIN      
        1 ccgaactcca gaagtttatt tgacgttgtg gcagcatttc ttgggcagcg gagtacttct
       61 tcattttgat gggatattga ataagggaca tggaaaacgg cgccttgatc gttaccaaag
      121 gcctcaccaa ggtgtacaaa cgggtaactg cactcgagga tttgtgtctg caggttggcc
      181 gtggagaggt gtatggccta ctgggcccga atggttcggg gaagacgacg acgattcgac
      241 tgttgctggg gctcttgcga ccgacggcgg gttgggcaac ggtagcggga ttcgactcgt
      301 ggcggagcag tctcgacgtc aggaggttgg tgtcatacct gccgggtgag ttgaggatgt
      361 atggatcttt gaccgggctg gggacactcc gcctcttatg cgacctgcgt gagggagccg
      421 gcctggaccg cgcggtggcc atcgccgagc gcgtcatgaa gctcaacctc aagcgaaaaa
      481 tacgaacgta ttcgacggga atgaagcaaa agctcgcact agcccaggtg ttctccgacc
      541 cggtcgacac gttgatcctg gacgaaccaa cctcggcact cgatccctcg gcgcggtcga
      601 tcgtgatcgg tttggtacag gaggctcggt ctggaggaca gaccgtgatc ttctcaggcc
      661 atgtcctggc ggaagtcgaa caggtcgccg atcgcgtggc gatcatgcgc aagggaaggc
      721 taatgcatgt cgaagacatg catgtccggc gaagcgtgcg tatgctgctc atccgattcg
      781 agggagggat tgagccgcag tttcccccag agctggagct ttcggtgcgc gaacgtaatg
      841 gcgagacgct acttttggaa catcggggcg atttgtcatc actcttgcga tggcttgctt
      901 cggaggccgt tgtcgacatc gcgatcggca ccgaagatct gcgcgcgctt tacgaccggt
      961 tccatggtct gaacgttccc gatgaggagg ctccggtatg aggccaatct gggctctctt
     1021 acgcaagcag atcgtggaat cgcggtggac gctagggctt tccgcagccg cgcttttcgg
     1081 gctgggatgg ctgtttgtct atgtgacgtc gctcaacgag gcggagatcc tccgtctgct
     1141 cgagtcggac tccgaaggcg gcggccgaat tcagtggatg agaaatctcg gggtcgcgga
     1201 ccagcccacg tcggtggcga tcatgatggc gttctggaat catccgttca ttgtgcttct
     1261 cttctccatc tgggcgatcg gccgtggctc ggccgcagta tccgccgaga tcgaacgcgg
     1321 cacgctggat ctgatcttgt ctcggccggt gtcgcggtct gcctatctgg cgtctcaggt
     1381 cttcgttgcg atcgtcggcc ttttgatcct gggagcagcg ttgctggcgg gcgcctcgat
     1441 cgccgtcggt tacaacgcgc tacacgaacc accaatttcc tggaccttgt tcaagcccgc
     1501 gctcaatttg tgcgccctcg gtctgccgat ctacggctat actctgctgg catcggccct
     1561 ggatcacgtt cgctggcggc ccacaaccat cgggtcgatc ttgactctgg gcggattcat
     1621 cgcttacgtc atctcgacga tacccgtact caaagatatg agctggaagc cttggttgga
     1681 gcggatttcg atcttcaagg cgtataaccc agttgagctc gttacgaccg gcgagacact
     1741 ccagttcaat ctggcgctgc tctcggggat tggagtgtca tgcatcgcgc tggcattcgt
     1801 ggcattcgcc gtacgtgacc tgccaacgaa cggctgagga tcagattata tgatcggtca
     1861 acaccgtttc gacgatgcgg ttgtcggggc gggaatcctg ggactggccc atgcctatca
     1921 tctcgccagg cgcggacgcc gggtgatcgt cttcgaacga acgccccgcg ccatgggcgc
     1981 atcggttcgg aatttcggta tgctctggcc aatcggccag ccgcccggtc ggctacggca
     2041 aatggccatg cggagtctcc agcattggct ccaagttctg gcgtccagta gcctgtggca
     2101 cgagcagacc ggctcgctcc acctggcgta tcgggacgac gaagcccagg tcttgagaga
     2161 gttcgggcga gaactgtcgg cccaaggcga agccgtagat ctgctcattc cagatcaagt
     2221 ccaagagcgc acccgcgcgg taaagcgcga cgggcttcaa ctcgcacttt ggagtccgca
     2281 cgaggtatgt atcgaccccc gcgaagtcat cgcgggcctg ccgccctggc ttgccattcg
     2341 cttcggcgtc gtttttgaat tcggtcgaac gatcacggcg gtcgaaacgc gccgggtcat
     2401 ctccggccag gggagctggt cggccgagcg agtctggatc tgttcgggcg acgaactgcg
     2461 tttgctcttt gccgaccagt tcgaggattg tgggctctta cgctgcaagc ttcagatgat
     2521 gcgctcacag ccctacgggc aagaacggcg gatcggtccg atgcttgcga gcgggctgac
     2581 gttgcgtcat tattccgcct ttcagaactg cccgacgctg ccggccttac gccgtcgtat
     2641 cagcgaggaa tcaccgtggt tcgatcgcta tgggatccat gtcttggtgt cgcaaaacgg
     2701 ccgtggagag ctgaccatag gcgattccca tgagtacggc gccgcgatcg agccgttcga
     2761 caaaaccgaa gtcgacgaat acatcctgga ttacctgaaa acgtttgtcg acgtgccagg
     2821 cctgcggatc gcctcacgct ggcacggaac ctacgccaag catcccacgc ttccgtacgt
     2881 gctcgtccac cctctccccg gggtaacggc gatcacagga gtggggggag ccggcatgac
     2941 cctttcgttt ggcttggcag aacaagtggt cgcgcgcgag ctcggtgaac ccgaggcctg
     3001 atcgattacg ggtagaatag ccatgaaaga tatcgcgctt gtggtcttcg acatcgccgg
     3061 taccactgtg aacgacgacg actcggtgaa tcgatgcctg cgaggttcgc tctcggcagc
     3121 tgggctccta gtcacggcgg cccaggtcaa caccgtcatg gggcttccca agcccgacgc
     3181 gattgccatt ctgatagggc agtctccctt acgagacgag ttgcgcgggc gggtcgatct
     3241 tatccaccga gacttcgtgg cccggtcgat cgagtttttt ggtagtgatc cgtcggttca
     3301 cgaagtaccc agcacgacgc gggttttcga gacgctcaaa cggtccggca tccttgttgc
     3361 gctcgacacg ggcttcaacc gctcaattac tcaagtgatt cttgatcgat tggactggtc
     3421 gggcagccgc ttgatcgatg caaccatctg cagtgacgag gtccgtcgcg ggcgccccca
     3481 tcccgacatg atcgaaacgc tcatgtcacg gctcggaatc gctgatccga aacgggtggc
     3541 gaaggtcggt gatactcctt ccgacctcca ggagggtcac gccgcgggtt gcaggctggt
     3601 tgtcggcgtg accgcgggca cgcatacgcg gctccaactc gagtcctgcc ctcatcatta
     3661 tctcatcgaa acgattgcag attttcccgg attgctcggg ctcgagatcg gttgaatcgg
     3721 gctgtttgcg taacgcattt catcgccaca agtacataag gcaagaacga aaaagaagct
     3781 ggcgacgccg cgcacttggg agcgcgaaat cgccaacttc atctagggct tacgtgatat
     3841 tggtcgaatt cgatcctgtt tattacgggt tgttcggttg cggcggggcg ttcttcttgt
     3901 cttttccttg gagtgaatcg aggtctaatt tgttaaagaa ttcgcccaca tgagcgggct
     3961 gggcacgagc ggtcgccgta tcctcgacat tggactcttt gacgactcgg tcgcgcactt
     4021 catcaaggag cgggaaaagc tgctcgtaga tgattcgctg acggggggtt gtggcggcac
     4081 cgaaccgtag gttgtaaatg tgcatgaagg caagcaagtt gccgagcgag gatgtcttga
     4141 ccatgcgtag ctggtcgaac gcttcccgcg tatcaggttt ttcgagcatc cgcacgagcc
     4201 ccgccaaggt cttgataaac ttggaggctt cttgactttg cgcgagatta tcgaagggag
     4261 ttgactcgat tttcgctcga aggctgctga ccaggttttt cgccctggtg agtgtttccg
     4321 cggagatgtc gccttcctcg tcctcggctc gcactttatc cacgatttct tcgaagtttt
     4381 tcttatcgct agcaaaacgg tcgttattaa gcgcactagg ccatttggac gctgctttca
     4441 cctggctgag gacaatcgtg actgcctcgg aggcattgcg aaacgggata tccttgatga
     4501 tctgggcgtc gactgggctc gtggcgattt tcaatgaaga gctggaaatt cgcgggtcgc
     4561 taagctggtc aagtgcggcg ttgagcgcat cgccgttttc gacatcgcgc gccgtgggat
     4621 gttcgcgaag attcttcaga agctcgttgt agacggcttt gtttttggcc gcatcgccgt
     4681 cgcgcttggc cacgtactga cgtgtggcct cgaggtgcgc ttcgtagaga tattggttcc
     4741 aacgcatgaa cgtatcggtg ttgatcgcac gggcttgtgc ggtgtccagg ttgtagaccc
     4801 ccgctcccat gttaaagtag ccgagcccac gggcgtaatc acctccaggc gttgcgcccc
     4861 agccgcccca accgccccag ccccagccct gagcgtggac ggtcccttct gataaccaca
     4921 gaaccagcgc cagtcccagc gccacaggaa taggtcggag cattgcaaaa cctcccgatg
     4981 gcaaagcatt agcttcagcc gatttgggtg acaaggtcag gagcggtcaa agtcaatcat
     5041 cggtcgagcg cgagcgactt taatttatca tacaatcccg gtgccaaagc agtaagaggc
     5101 tgttggcgat gaattatgtc ctggatcgcg gcgaagttcg gtcagcgcaa tcgggcagaa
     5161 tctgcggcgt gaaaaacgtc aaaaatcgtc gagtggcacc agatcaattt cggaatcgga
     5221 tcgggcacgg ggaggtaagg ctgcggcttg gacgtttgcc ggtgcgccga ctggacgcgc
     5281 cgctttcggg ggctgactcg gacgcgaaat cattgcgcct gaatttcgag gttcctcgct
     5341 ttccgcctcc agacggaaca ggacgggccc gatcgctagc tcgtcgccgg gatgcagtcg
     5401 tatctcttcg accagccggc cgttgacacg aacgccattc ctgctgccca gatcgcggat
     5461 caagacgcgg tcgtaagcca tggccaggca acaatgccgt cgtgaaatct ttggcgaatc
     5521 gatccggacg tcacattcga gatgtcggcc gatcaagagg accggtcggt gcagggcgac
     5581 ctggggcgca gttcccggga tcatggggac gagtcggaat gacattcggc ggcgttctcc
     5641 gaggcgtggt tcggcaccag tggagctggt tctccatcac tcgagccgga gtcacccgcc
     5701 atcttgacga tgacttgaat tcgacacttg tcagcgactc cgttgggaag cagcttgcgg
     5761 accgccgcag cgaaggcatt ctcaccgccg ttccccaagg gaaccgcgtc ggccacgcgg
     5821 gcgtcaagag aacctcctcg atcctcgtcg tcagccgatc gcgccatttt tccgagccca
     5881 cctgcctcgg cgatcgtctg ctcgtggccc tgtccgttct ccaggcggta gcgtatgtgc
     5941 gcaatcgaca gctcatcgcc aggtcgcacc cgcccgatct ccactcgttg gccgttgatc
     6001 cggatgccgt tggtgctgcc caagtcgcga accatcacgt caccactctc atgcgtcatg
     6061 cagcagtgat gtcgagatac tcgcagcgaa tcgagccggg tgtcgcacga cgggtgccgc
     6121 cccaccacca ccatggcgcg gtccagaatg atgtctggac cttcatcgag ggccacaagt
     6181 cgtgccggca tggtggtaga tcctcgactt aagcaattga cggggataaa gactatcggc
     6241 cgccaagact taaaccccta ttatagatca tccctttcgc gaatgaccag cgcgaaaact
     6301 caaatcgcca ggacccgatt ctcacgatcg catgcatcgc ctgcaccatt tcgttccttt
     6361 ttttcaccaa cctcacccac tagccgttcc gatcgacgtg gcaatcttct gctctaaata
     6421 taagatgggt ggcctgaaaa ggatacaagt tttccactat ttcctgccaa tgcgtgctcc
     6481 atttgcgggc cagtggaacc gatcggttcg cgcgccgtcc cgtaattcga atccataaca
     6541 tctgtcatta atgcgaaccg tcgccagttc atcaaaactg ttatatctcc aatcgttgcc
     6601 gcgggcacaa accgtgcgaa atttccttct cagcacggtc agtgccgagc tgacacgtct
     6661 ctctgatgat agtcaatcgg ctaaaccgga cgacattatc caag
//
LOCUS       DATAQL010000389         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_342605, whole genome shotgun sequence.
ACCESSION   DATAQL010000389 DATAQL010000000
VERSION     DATAQL010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342605"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VHS97_06730"
     CDS             complement(<1..256)
                     /locus_tag="VHS97_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX3447930.1"
                     /translation="MNSAPAHDTLVSAEAGGPPVSTVVVQRVPADRVQKFLECQRGLI
                     QAAEQFPGCRGTELYPPPDDRHSEWVAILHFDDHESLEGWL"
     gene            complement(353..>3122)
                     /locus_tag="VHS97_06735"
     CDS             complement(353..>3122)
                     /locus_tag="VHS97_06735"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3447931.1"
                     /translation="QEVDDCTRGQHEMDEEGISRAYRRQLAENYFYKFMLHVALAIDP
                     KKIAAANLSAANHHDRPLSHGTQEYTEYPELFPVTKPIIKRAAFVQTSGEVRYTQDVP
                     LPVGGLHAAMVKSSRAHAHFSLTRKAATLEALEALLRQKYREFKALITVADIPAGGEK
                     LIGLGEDDPVFSDGIVTSVGAPIALAVAETIATAREAAAFIENECIAYEDLPAVLTLD
                     EAIKQNTAMPMIRKSKNPDEDVEARIPSLTRPGSDLSWLSNPDAGLADTEQVTGSFLT
                     NAQAHFYLETMCALAIPGMYDQMTVYNSTQNPNGNQGAIARALGVKTNQITVIVEQIG
                     GGFGAKQHRASLPGAQAAVAARKLNRPVRLLYDRATDSLMVGKRHPYLGEYHMAFTRE
                     GILEGVRIDLHSDAGDTYDCSFAVLDLSLLQADGCYMAKTFQANGTVYRTNKTSNTAF
                     RTFGNIQPYVFREDAMEHVAHQLSKTLGRKVLPEAVRRKNLYRTGDMKDYDLTHHGQR
                     LRFCNIREIWDDLYQWSDFENREREIQEFNRANRWRKRGIAMIPQKYGIAFSEPRGSL
                     NASSALVNINMADGSVFVQHGGVEMGQGLHTKIAQVAASTLGIPLEWIRIGGNNSDAI
                     VNAPATAASTGYDLNAGAVDKACRVLRTRLEDFCRDLEQFTPHDCIEGWRTGWADKWQ
                     EIVFKAWFNRVNLSAAELYKNPHYMGVSDRQPTGHPYFYFVYSAAATEVEVDVLTGEF
                     TILRADVLYDAGKSPNPAIDIGQLEGGFVQGVGFCTTEETVFNEEGGLVTDNIWSYKP
                     PCTKTIPLDFRLRFYPVDEARNALEEQAELHAVKSAKTTGEPSMTLGITAFFAIKRAI
                     MDVRRELTGNDDWLTMDVPATCQRIQMNCGVSTDALTLGGPEPRVGRNGNGHVEHANP
                     IPFVSGTP"
BASE COUNT          574 a          941 c          932 g          675 t
ORIGIN      
        1 cgagccagcc ctcgagcgac tcgtgatcgt cgaagtggag gatcgctacc cattccgaat
       61 gccggtcatc cgggggcgga tacagctcgg tcccgcggca acctggaaac tgctccgcgg
      121 cctggatcag cccgcgctgg cattccagga acttctgcac acgatcggcg ggaacgcgct
      181 ggacaacgac cgtggagact ggcgggccac cagcttccgc ggaaacgagc gtgtcatggg
      241 cgggtgccga attcattcgt tcctcctgcg ctcacagtcg cgtaatcact tgcttggccg
      301 cggctcgcac aattgcaatg cgtggagctg cgctgggacg atccccgaca catcacggcg
      361 tgcctgaaac aaagggaatt ggattagcgt gttcgacgtg cccattgcca ttgcgaccta
      421 cccgtggttc aggtccaccc agggtcagtg cgtcggtcga aacaccgcaa ttcatctgga
      481 tgcgctggca ggtggcgggc acgtccatgg tgagccagtc atcattgccg gtcagttcgc
      541 gacggacgtc cataatggcg cgtttgatcg cgaaaaacgc cgtgatcccc agagtcatgc
      601 tcggctctcc agtcgtcttc gctgacttga cggcgtggag ctcagcctgt tcctccaagg
      661 cattgcgcgc ttcatcgacc ggatagaacc gcaggcggaa atcaagaggg atcgtcttgg
      721 tgcacggagg cttgtagctc cagatgttgt cggtcaccaa accgccctcc tcattgaaga
      781 cggtttcttc ggttgtgcag aatccgacac cctgaacgaa tcccccctcg agctggccga
      841 tatcgatcgc ggggttgggc gacttgcccg catcgtagag cacgtcggcc cggagaatgg
      901 tgaactctcc ggtcaggacg tcgacctcca cctccgtcgc cgcggcggag taaacgaagt
      961 aaaagtaggg atggccagtc ggttgccggt cgctcacccc catataatga ggattcttgt
     1021 acaactcggc ggcgctaaga ttgacccggt taaaccaggc tttgaacacg atctcttgcc
     1081 acttatccgc ccagccggtc cgccatccct cgatgcagtc gtggggggta aactgctcca
     1141 ggtcgcggca aaaatcttcc aaccgagtgc gcaagacccg gcaggccttg tcgacggccc
     1201 cagcattaag gtcgtaaccg gtcgacgcgg cggtggctgg tgcattgacg atcgcgtcgc
     1261 tgttgttgcc gccgatgcga atccattcca gggggatacc cagtgtgctt gccgctacct
     1321 gggcgatctt ggtgtgcaaa ccctggccca tctcgacgcc gccatgctgc acaaacaccg
     1381 acccgtcggc catattgatg ttgacgagcg cgctcgatgc gttcaaggaa ccgcgcggct
     1441 cagaaaacgc gatgccgtac ttctgaggga tcatggcgat gccccgcttc cgccagcgat
     1501 tggcccggtt aaactcctgg atttcccgct cgcgattttc aaagtcagac cattggtaca
     1561 ggtcgtccca gatctcgcgg atattgcaga atcgaagtct ctgcccgtga tgtgtcaggt
     1621 cgtaatcctt catgtcgccg gtccggtaca gattttttcg gcggaccgct tccggcagca
     1681 ccttgcgacc aagcgtcttg ctcaactggt gagccacgtg ctccatcgcg tcctcacgaa
     1741 agacatatgg ctggatgttg ccaaacgtgc gaaacgcggt attgctggtc ttattggttc
     1801 gatagactgt accgttggcc tggaacgtct tggccatata acagccatcc gcctgcaaga
     1861 ggctcagatc caacaccgcg aacgaacagt cgtaggtgtc gccggcgtct gaatgcaggt
     1921 caatgcgcac gccttcgaga attccttcgc gagtgaaggc catgtggtat tcacccaggt
     1981 agggatgccg tttgccaacc atcagactgt cggtggcgcg gtcgtagagg aggcgcaccg
     2041 gccggttcag tttccgcgct gccaccgcgg cctgggcccc tggcaacgac gcgcggtgct
     2101 gtttggcccc aaagccgccg ccgatttgct cgacgatcac cgtgatctgg ttagtcttga
     2161 ccccaagtgc cctggcaatg gcgccttggt ttccgttggg gttttgagtc gagttgtaca
     2221 cggtcatctg gtcgtacatt cccgggatgg ccagggcaca catggtttcc aagtaaaagt
     2281 gcgcctgggc attggtcagg aaagatcctg tgacctgctc ggtgtcggca aggccagcgt
     2341 cggggttgct cagccagctt aggtcgcttc caggacgtgt gagcgaggga atccgcgcct
     2401 cgacatcttc gtcggggttc ttcgatttgc gaatcatcgg catcgcggtg ttttgtttga
     2461 tggcctcgtc cagtgtcagt acggccggca ggtcctcgta ggcgatgcat tcgttttcga
     2521 tgaacgctgc cgcctctctc gccgtggcga tggtttccgc gaccgccaag gcaatgggcg
     2581 cccccacaga ggtaacgatg ccgtcgctga acaccggatc gtcttcccct aagccgatga
     2641 gtttttcgcc ccccgccggg atgtcggcca ccgtgatcag cgccttgaac tcccgatact
     2701 tctgccggag caatgcctca agggcttcca gcgtggcggc cttccgcgtg agggaaaaat
     2761 gcgcatgggc ccgcgagctc ttgaccatcg ccgcgtgtaa gccgccgacc ggaaggggga
     2821 catcctgcgt gtaccgtacc tcgccggatg tctggacaaa cgcggctcgc ttgataatcg
     2881 gctttgtcac cggaaacagt tccggatatt cggtatactc ctgcgtgccg tgtgacagtg
     2941 gtcggtcgtg atgattggcc gccgataaat tcgccgctgc tatctttttg ggatcgatgg
     3001 cgagcgcgac gtgcagcatg aacttgtaga agtagttctc cgcgagctga cgccggtacg
     3061 ctcggctgat cccttcctcg tccatctcat gctggccgcg ggtgcagtcg tccacttcct
     3121 gc
//
LOCUS       DATAQL010000390         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3855570, whole genome shotgun sequence.
ACCESSION   DATAQL010000390 DATAQL010000000
VERSION     DATAQL010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3855570"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..962
                     /locus_tag="VHS97_06740"
     CDS             354..962
                     /locus_tag="VHS97_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447932.1"
                     /translation="MKKASGLSVGAIGFTIVIIALDFAVIRAACLSPRPEERASPGAP
                     LLPGMLAGRLLSRGPDGWAVFAFFLLPMINALLIGIYRLWRENRTVGTVGFVIAGAAA
                     TLAVFTSCLISPRTAIGILTPISRPIALASFHGLSRLVGNAAWPNRALEWTYAVIFAV
                     LIPIAFFCIPPLVVGVIGGRVARFFETSRSPQRARQVNTVVL"
     gene            1364..2074
                     /locus_tag="VHS97_06745"
     CDS             1364..2074
                     /locus_tag="VHS97_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447933.1"
                     /translation="MGHSTTERPSGPNSSHDQFVLASILAPTAGAGLCMAFAIGFWVR
                     GLIDGDVSRSARAGPELAAAGLPATAHLDHPEAVETERAEPERLAETQVQTPIVAAPP
                     TTPPAEAAPLAAASTKPPPHRPLGPVRDQTRAPAVRDYTPAPAAPQRFVLRQAARRAS
                     LPFVLPEKPSFALADLKLDEPEACGQTEQVCAVDRSLNTALTWAKSPGAASEQARKEG
                     KLVFLIHVSGNFEDPGFT"
     gene            2147..>3261
                     /locus_tag="VHS97_06750"
     CDS             2147..>3261
                     /locus_tag="VHS97_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447934.1"
                     /translation="MSAHQQVGSFEVVNHAGVLAKNGGNVASYFCTPDGRVIHAVTGP
                     VPADELLAAARWAVDVSSGGRALDDPERIGAAHREELQRSSRPAASPAQQKVHRLLAS
                     QPLPPLNEVYQTVFEEILGQRVSPPDTERVQAERAFAAARRAKLPVLLILHKRRDNQT
                     VLDEWNRMVASPRTVVSDQAEEEGEDEVGLVGRGGRETLGPAAGRVRRPAPSASPLFP
                     GIRHRVRSHRRPSVPAVASSGDLATTKEVSCSNPLTALARSYVVVALSLEELPALSHQ
                     LGIPPYAAPDRGSPLFVIARSDARQLSAVTTWDKSNQLAYAMAQGIVQEAKEHERTPE
                     QLRTLLAVVDPVDAGLGDQVRKLLGGPDNAGSHRAGQV"
BASE COUNT          572 a         1042 c         1033 g          614 t
ORIGIN      
        1 tcgggggcga caagcaggaa tgggacattg aggggaaccg ggagaagaaa gactgattga
       61 tcctcccaag accttcggat ctcggaccag tactgaggaa cactcgatcg gagagccccg
      121 acagattcgg atcgccgggc ggttcacgtt tgcttcaaga agtccacagt gcgccaaggt
      181 cgcccgggtc ctcgagcagg aggaacggcc gcgcggtcga cgagcttgct acggtaaacc
      241 acgcttggaa catccgcgag ctattcgaca cgtagctaca cttgacatgg ttgtgcatcc
      301 agttacctgg ccttcgaaag cggggtgacc ccgaatcgtc aaggagcatg gacatgaaaa
      361 aggcctcagg gctctctgtg ggtgcgatcg gattcacgat cgtgatcatt gcgttggatt
      421 tcgcggtgat ccgggccgca tgcctcagcc ccaggccaga agaacgggcg agtccgggag
      481 cacccctact cccaggcatg ctggccggtc gactcctcag ccgtgggccc gacggatggg
      541 cggtcttcgc tttcttcctg ctgccgatga tcaatgccct cttgatcggc atctaccgtt
      601 tgtggcgaga aaatcgcacg gtcgggaccg tcgggtttgt catcgccggc gccgcagcta
      661 ccctcgccgt tttcacatcc tgcctgatct cgccccgcac ggcgatcggc atactcactc
      721 ctatcagccg cccaatagcc ttggcgagct tccatggtct gtcgcggctg gtcgggaatg
      781 cggcgtggcc gaatcgtgcg ttagagtgga cttacgcggt catcttcgcg gttcttatcc
      841 ccatcgcgtt cttctgcatc ccgcctctcg tggtgggtgt catcgggggg cgggtcgccc
      901 ggttttttga gacatcacgt tccccgcagc gcgctcggca agtgaacaca gtcgttctgt
      961 aggcgcgcgt cggtctgatc gaccgacgat cccagaattt gaggcaccca cgaacaatct
     1021 gttcgatcgg aacaatcgac cagcgcatcg ccttgcacgg ccgcgaggcg gctggagaca
     1081 acgtgtctcc tgtcgactgt cgtgtttgcg ccagcgcttg acgccagagg gtcgccggtt
     1141 cgctgcgtcg agatggcgcg ggggcgtgat cagatccttg cggtttacga cgatcggctg
     1201 ccgccacgac tgaggctttc gctcaatttc agccagcacc tcaccaggtc tgaccccctg
     1261 gtgcgcagtt tttttccgat cgctgaactc cgcgcccatc gatcccgtat attgggtaga
     1321 tggtctctcg ttacgctgtt cactcctttt gggaggcgcg aagatgggcc actcgacgac
     1381 cgagcgcccc tccgggccga attcctctca cgatcagttc gttctggcaa gcatcctcgc
     1441 ccctaccgct ggggcgggct tgtgcatggc cttcgccatc ggattctggg tgcgcgggtt
     1501 gatcgacggg gatgtgagcc gatcggcccg agccgggcct gaattggcgg cggcgggtct
     1561 gccggcaacg gcgcatctcg atcatccaga agcggtcgag accgaacggg ccgagcccga
     1621 acgcctggcc gaaacccagg tgcaaacgcc tatcgtggcc gcgccaccaa caactccgcc
     1681 tgccgaggct gcccctctcg cggctgcctc gaccaaaccg ccgccccatc gaccgctcgg
     1741 gccggtccgc gaccagacac gcgctcccgc tgtccgcgac tacacaccgg ctcccgctgc
     1801 accgcaacga tttgtgttgc gtcaggcggc tcgtcgggcg tcgctgccgt ttgttctgcc
     1861 ggagaagccg tcgtttgccc tggccgattt gaagcttgac gaaccggagg cctgtggcca
     1921 aacggagcag gtctgtgcgg tggaccgctc gctcaataca gcgttgacct gggccaaatc
     1981 gcccggcgcg gcctccgagc aagcccggaa ggaaggcaag ctcgtcttcc tgatccacgt
     2041 ctccgggaac ttcgaggatc ccggctttac ctgaaataac gcacaaacat tcagggcggt
     2101 cgccctgaag gatcccaatg tcgcgcgtta tttcgtcgcg cactttgtgt cagcgcacca
     2161 gcaggtgggc agcttcgagg tggtcaacca tgctggcgtc ctggcgaaga acggcggcaa
     2221 cgtcgcgagc tacttctgca cgcccgacgg ccgagtcatc cacgctgtga ccggaccagt
     2281 tcccgccgac gagctactgg ccgccgcacg atgggcagtg gatgtctcgt ctggcggcag
     2341 ggccctggat gatcccgaac gaatcggggc ggcccatcgc gaggagctcc agcgctcgtc
     2401 gcgaccggcc gcgagtccgg cacagcaaaa ggtccaccgg ctgctggctt cgcagccgct
     2461 gccaccgctg aacgaagtct atcagacagt cttcgaagaa attctgggcc agcgcgtcag
     2521 tccgcctgat accgagcggg tccaggccga gcgggccttt gcggccgccc ggcgtgccaa
     2581 gctgccggtg ttgctcatcc tgcacaagag acgcgacaac cagacggtcc tcgatgagtg
     2641 gaaccggatg gttgcgagtc cccgcacggt tgtctccgat caggctgagg aggaagggga
     2701 ggacgaagtt ggtctggtcg gacgaggtgg tcgggagacc ctcggtcctg ccgcgggtag
     2761 ggtgaggaga cccgcgccca gcgcatcacc gctttttccg gggattcggc atcgagtacg
     2821 atcacacagg agaccttccg tcccggcggt ggcgagctcg ggagacctcg ccacaaccaa
     2881 ggaggtctct tgctccaatc cgctaacggc cctggcgcgc tcttacgtgg tcgtggcgct
     2941 aagtttggag gaattgccag ccttgtcgca tcagctcggg atcccaccct acgctgctcc
     3001 cgaccggggc tcaccgctgt ttgtcatcgc ccgcagcgat gcacggcagt tgagtgccgt
     3061 cacaacctgg gacaagtcca atcaactggc ctacgcgatg gcccagggga tcgtgcagga
     3121 agcgaaggaa catgagcgga caccggagca gctccggacg ttgttggccg ttgtcgatcc
     3181 ggtggatgcc ggactcggcg accaggtgcg caagctcctg ggcgggccgg acaacgccgg
     3241 gtcacaccgc gcgggccagg t
//
LOCUS       DATAQL010000391         4063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_954152, whole genome shotgun sequence.
ACCESSION   DATAQL010000391 DATAQL010000000
VERSION     DATAQL010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954152"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VHS97_06755"
     CDS             <1..1463
                     /locus_tag="VHS97_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3447935.1"
                     /translation="LVARHGPLVWNVCRSISSDPHAAEDAFQATFLILVRKAGSIRRR
                     ETLGPWLYGVARRVAVRSKVAIRRQRQKEERGAEMKATSQPDQTTQEEVESLHEEIGR
                     LPEKYRSAVVLCHLEGCTHADAARVLKCPTGTVNVRVARARELLRDRLARRGLAYSTA
                     LAGAALVSDTAAAAMAVKLAQTTIKAAMQVAAGKSVTAGGVSAAVAQLTEGVLKTMTL
                     KKVTVAAALVLTAGSVSSGIALLAMGPGPRPANAGPVAASPAQDPNAQKDDSRPKVGN
                     KERVLSINNLSRIGLAMHSFVANSKETRYPPAAIRKDGKPLLSWRVAILPYLGQQALY
                     DKFRLDEPWNSPHNKTLLNQMPDIYAPVVRTDEPRGSTYYQVFTGPGALFEDEVGPGI
                     QDIKDGSANTFMVVEAGGPIPWTKPEDISYDKEKPLPKLGRQFDDGFHAAFADGSVRF
                     LSNAINPDIVRRLITRSGGEFVTVDMLLPDPRPVDE"
     gene            1685..3457
                     /locus_tag="VHS97_06760"
     CDS             1685..3457
                     /locus_tag="VHS97_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447936.1"
                     /translation="MTRSVVFTVGAAKPEAWISLGALVLALGTVELATAGEEPPSQDG
                     ATSFPVVIRVDAAKTIGEMQPVWRFFGYDEPNYTYMKDGQKLLGQLRSLSSEPVYIRA
                     HNLLTSGDGQPALKWGSTGVYTEDDQGRPRYNWTILDHIFDTYLERGVRPYVQIGFMP
                     EALSAHPQPYQHHWTPGGRTSISTGWAYPPKDYGKWAELVFQWVRHSVERYGQPEVER
                     WYWEVWNEPNISYWRGTPAEYHKLYDFAVDAVKRALPTARVGGPHVAGPRSAGGGKFL
                     REFLDHCVRGTNYATGQPGAPLDFIAFHAKGVPVFVDGHVRTGIAAQLEEIDRGFAIV
                     ASYPELREKPIIIGESDPDGCAACPATIYPQNGYRNGTLYASYTAASFARKYLLADKH
                     GVNFEGAVTWAFEFEDQPYFAGFRVLASNGIPLPVLNVFRMFGLMGGQRLAVESAGAV
                     ALEAIRKDGVRARPDVAALASLQNGKMCVMVWHHHDDDVPGPAAEVELSLNPLVDTGS
                     TTLLQHFRIDADHSNAFAAWKRMGSPQQPTPGQYAELERASNLALLTSPEWLRPQSGK
                     LTLKFSLPRQAVSLLVLDRAAKPK"
     gene            3522..>4063
                     /locus_tag="VHS97_06765"
     CDS             3522..>4063
                     /locus_tag="VHS97_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447937.1"
                     /translation="MSDWLMRVIKAEAERLIELAAEDDELRADLRELAESILAATAPT
                     QPKIGTPSPVQLSEASTDQGTQTEEPLKELTFGRPSLSRGSAPPVTPAVNQPQTKHDD
                     LIQLENRCRRKSEAARWAAERLRRAREGNAFQVENPPIDPETVEWADRLTDCFYWMQS
                     SPDSSPADLSLLDNVGGCFEA"
BASE COUNT          778 a         1228 c         1288 g          769 t
ORIGIN      
        1 ccttggtggc acgccatggt cccttggttt ggaatgtctg ccggagcatt tcgtcggatc
       61 cacacgccgc cgaggacgct tttcaggcca ctttcctgat cctggtccgc aaggcgggtt
      121 cgatccgccg gcgtgagaca cttggcccgt ggctgtacgg cgtggcccgg cgagtcgcgg
      181 tgcgcagcaa ggtcgccatc aggcggcagc gacaaaagga agagcggggc gcggaaatga
      241 aggccacttc ccaaccggac cagacgaccc aggaagaggt cgagtcgctt catgaggaga
      301 taggccgtct gcccgaaaag taccgctctg ccgtggtcct gtgtcatctg gaagggtgca
      361 cccacgccga cgcggcccgc gttctcaagt gtccgacagg tacggtcaac gttcgcgtgg
      421 ctcgggcgcg ggaactgctg cgcgaccgcc tggcccggcg gggcctggcg tactcgacgg
      481 ctctggcagg cgctgcgctc gtttcagaca cggcggcggc cgccatggct gtgaaactgg
      541 cccagacgac catcaaggcc gcgatgcagg tcgcggcggg caagtcggtg acggccggag
      601 gcgtttcggc cgcagtggct caactgaccg aaggagttct gaaaacgatg accttgaaga
      661 aagtcacagt cgccgcggct ctggttctca cggcaggatc ggtcagctcg ggaatcgccc
      721 tcctggcgat gggcccgggg ccaaggccag ccaatgccgg cccggtggcg gctagcccag
      781 cacaggaccc caacgcccaa aaggatgatt ccagaccgaa ggtcggaaac aaggaacggg
      841 tcctgagcat caacaacctg tcgaggatcg gcctggccat gcacagcttt gttgcgaatt
      901 ccaaggagac tcgctatccg cccgcggcca tccgcaaaga cggcaagccg ctcttgagct
      961 ggcgcgtcgc gatattgccg tacctcggcc agcaggccct ctacgacaag tttcgtctcg
     1021 acgagccctg gaacagcccg cacaataaga cgttgctcaa tcagatgccc gacatctatg
     1081 ccccggtcgt gcgtaccgat gagccgcggg gatcgaccta ctaccaagtg tttactggcc
     1141 cgggcgcttt gttcgaggat gaggtcgggc caggaattca ggatatcaaa gacggatcag
     1201 ccaacacgtt tatggtcgtg gaggcaggcg gtcccatccc gtggacaaaa cccgaggata
     1261 tttcgtacga caaggagaaa ccgttgccga agctgggccg ccagttcgat gacgggttcc
     1321 atgcagcctt tgccgatggc tccgttcgat ttctcagtaa tgcgattaac ccggatatcg
     1381 tccgtcgttt gatcacacgt agcggtggtg aattcgtgac cgtcgacatg ttgctgcctg
     1441 accctcgacc ggtggatgaa taaacaaggg atcgagcatg gcgacgaacc gatcacccgg
     1501 gagaaccggc tgccgcatgt cttgcgtcag ccggtttttg tcattgtgat ctctccatcg
     1561 tccatctggt tccgggctca ggctttgatc ccaaggacat ggcgtgttat acggtggaag
     1621 tcgggatcag agtgctggtc gacaacctga ggtcaaaatg ccatccggat gcgaggttcg
     1681 caccatgaca cgaagcgtcg ttttcaccgt aggcgccgcc aagccggagg cgtggatcag
     1741 tctgggagca ctcgtcctcg cacttggtac cgtcgagctt gccaccgcgg gagaagagcc
     1801 accctcgcag gatggcgcta cctcttttcc cgtggtcatc cgtgtcgatg ccgccaagac
     1861 aatcggagaa atgcagccgg tctggcgatt ctttggctac gacgagccca attacactta
     1921 tatgaaagac ggtcagaagc tgctcgggca attgcgatcc ttgagttctg aaccggttta
     1981 tatccgggct cacaacctgc taacatccgg tgacggtcag cctgcgctca aatggggctc
     2041 gacgggtgtt tacaccgagg atgaccaggg gcggcctcgc tacaactgga cgatcctcga
     2101 ccacatcttc gacacgtacc tcgaacgcgg ggtgcggccc tatgtgcaga tcggcttcat
     2161 gcccgaggct ctctcggccc acccgcaacc ataccagcac cactggactc ccggcgggcg
     2221 aacctcgatt tccaccggct gggcgtaccc gcccaaggat tatggcaagt gggccgagct
     2281 tgtttttcag tgggtccggc actcggtcga gcgatacggc cagcctgagg tcgagcgctg
     2341 gtactgggaa gtctggaacg agcccaacat ctcctactgg cgagggacac ccgcggagta
     2401 tcacaagctc tatgattttg ccgtcgatgc cgtcaagcga gccttgccca cagccagggt
     2461 cggcggtccc catgtggcgg gaccgaggtc agccgggggt gggaagtttc ttcgggagtt
     2521 tctcgatcat tgcgtgcgcg gcaccaacta cgcgacgggc cagccaggcg cgccgctcga
     2581 cttcattgcc ttccacgcca aaggggtgcc cgtgttcgtg gacggccatg tacgcaccgg
     2641 gattgccgct cagcttgaag agatcgaccg gggctttgcg atcgtcgcga gttatcccga
     2701 attgcgggag aagccgatca tcattggcga gtccgacccg gacggctgcg cggcctgtcc
     2761 ggcgacgatc tatccgcaaa acggctaccg caacggcacc ctctacgcca gttatacggc
     2821 cgcttcgttc gcgcgcaaat atcttctggc agacaagcac ggggtcaatt tcgagggggc
     2881 ggtcacctgg gctttcgagt ttgaggatca gccttacttc gccggttttc gggtgctcgc
     2941 gagcaacggc attcccttgc ccgtgctgaa cgtgtttcgc atgttcggct tgatgggtgg
     3001 gcaacgactg gccgttgaaa gcgcgggcgc ggtggcgctc gaggcgattc gcaaggatgg
     3061 cgttcgtgcc cgaccagatg tggcggcgct cgccagcctg caaaacggta aaatgtgtgt
     3121 gatggtttgg caccaccacg atgatgatgt gccgggcccc gcggccgagg tcgaactgtc
     3181 cctgaatccg cttgttgata cgggcagtac gaccttgctg cagcatttcc gcatcgacgc
     3241 ggaccacagc aatgcgttcg cggcctggaa gcgaatgggt tcacctcagc agccgacgcc
     3301 cggtcagtac gccgaactgg agcgggcgtc gaacctcgcg cttttgactt cgccggagtg
     3361 gctgcggccg cagagtggca agctgaccct caagttcagt ctgcctcgac aggcggtgtc
     3421 tttgctcgtg ctagaccggg ctgcaaaacc gaagtgattg ctcgagactt ctcgcttggc
     3481 tggctcattc ttccgggtgt ctctggtgct gcgagggggg tatgtccgac tggctgatga
     3541 gagtgatcaa ggccgaagcc gagcgcttga tcgagctggc ggcggaggac gacgagctcc
     3601 gtgccgacct cagggaattg gccgagtcga tcctggcggc gacggcgccg acgcaaccga
     3661 agatcggcac tccgtcgcca gtgcagctat ccgaggcctc gaccgatcaa gggactcaga
     3721 cggaggaacc gctcaaagag ctcactttcg ggaggccctc gctttcgcgg ggcagcgccc
     3781 caccggtgac gccggcggtg aatcagcccc aaaccaaaca cgacgatctc atccaactcg
     3841 aaaaccgctg tcggcggaaa tccgaagcgg ctcgctgggc ggctgaacga ctgcgccggg
     3901 ctcgcgaggg gaacgccttc caggtcgaga acccgccgat agacccggag accgtcgaat
     3961 gggccgaccg gctcaccgat tgtttctact ggatgcagtc gtcgccagac tcgtcgccag
     4021 ccgatctctc gttgctcgac aatgtgggcg ggtgcttcga agc
//
LOCUS       DATAQL010000392         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5229400, whole genome shotgun sequence.
ACCESSION   DATAQL010000392 DATAQL010000000
VERSION     DATAQL010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5229400"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VHS97_06770"
     CDS             complement(<1..465)
                     /locus_tag="VHS97_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447938.1"
                     /translation="MIASVNRGRLQIRLARLLIVCAALGALAGCKPRGSSATFRSERP
                     ELADPRNDPMSLTWAILQLEPGRWREGYAQLVEVAARAPRIAWQGGCRRGAGEWAASE
                     GTKDRGRAGGVDTLVADLLGATLVDAQDELWMAWRALERAGYPEPARNWLTEP"
     gene            complement(517..>1554)
                     /locus_tag="VHS97_06775"
     CDS             complement(517..>1554)
                     /locus_tag="VHS97_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3447939.1"
                     /translation="RIVIDRPGIETKNVVGVLEGAGPHADETVVIGGHYDHLGHGGLT
                     SGSLAMFSSDIHNGADDNASGTTMVLEMARRLGARRDPLPRRVVFIAFSGEERGLLGS
                     QYYVSHPLFSLKKTVMMINCDMVGRLNAKNELTMIGTGTSPGIDALVEILGKSAGLTI
                     KKVNGMSDGFGGSDHESFYNKNIPVLFAFTGIHSDYHRPSDDSDRINYPGMSRIADYL
                     ELITLDVVRRPQRPVFAKLGERPRPERTAAAPDRGRPMASLGVMPDYSHEEKDGLSLS
                     GVREGSPAAKAGLKEGDLIIKLGGLPVGTIYDYMASMEKHQPGDKVDVVVKRDGKQVT
                     LEATLGGRPRE"
BASE COUNT          283 a          522 c          459 g          290 t
ORIGIN      
        1 cggctcggtc aaccagttac gggccggttc gggataaccc gcacgctcaa gcgccctcca
       61 cgccatccag agttcgtcct gcgcatcgac caatgtcgca cccagcaaat cggcgaccaa
      121 tgtgtcaaca ccgcccgcgc gtccgcggtc cttcgtgcct tcgctcgcgg cccactcacc
      181 agccccgcgt ctgcacccgc cctgccaagc gatgcgaggt gcgcgcgccg ccacctcgac
      241 gagctgggcg tatccctccc gccaacgacc cggctcgagc tgtaatatcg cccaggtcaa
      301 actcatcggg tcgttgcgag gatcggcgag ctcgggccgc tcagaccgga atgtcgcgct
      361 cgacccccgt ggcttgcagc ccgccaatgc gcccaacgcc gcgcaaacaa taaggagtcg
      421 cgccaggcgt atctgcaagc ggccacggtt gacagacgca atcatcggca cgtcgtcctg
      481 agccgagcga gcggctctgg ggtcgagagc ggcgaatcac tcgcgggggc gcccgccgag
      541 cgtggcctcc agcgtgactt gcttaccgtc gcgcttgacc acgacatcga ccttgtcgcc
      601 gggttgatgc ttttccatgc ttgccatgta atcgtagatc gtgccaaccg gcaggccgcc
      661 cagtttgatg atgaggtcac cttccttcaa cccggccttg gcggcaggac ttccctcgcg
      721 gactccggac aggctgagac catctttttc ctcgtggctg taatcgggca tgacgccaag
      781 cgatgccatc ggtcggcccc gatccggcgc ggcggccgtt cgttccggac gaggcctctc
      841 acccagcttg gcgaagacgg gccgctgggg gcgccgaacg acgtcgagcg tgatcagttc
      901 gaggtagtcg gcgatccggg acataccggg atagttgatt cggtcagaat cgtcgctggg
      961 ccggtgataa tcgctgtgta tcccagtgaa cgcgaataac acagggatgt tcttgttata
     1021 aaatgactcg tgatcgctgc cgccgaagcc gtcggacatc ccgttcacct tcttgatcgt
     1081 aagcccggcc gactttccca gaatctcgac cagcgcatcg atacctgggc tggttccggt
     1141 accgatcatc gtcagctcgt tcttggcgtt caaccggccg accatgtcgc agttgatcat
     1201 catgaccgtc ttcttgagag aaaacagggg gtgcgaaacg taatactgcg agccgagcag
     1261 cccgcgctct tctccagaaa acgcgatgaa aacgacgcgc cggggcaggg ggtcgcggcg
     1321 agctcccaac cgtctcgcca tctcgagcac catggtggtt cccgaggcat tgtcatcggc
     1381 accgttgtga atatccgagg agaacatcgc cagcgatccc gacgtcaggc cgccgtggcc
     1441 caagtggtcg taatggccgc cgatcacaac cgtttcgtcg gcgtgtggac cagccccttc
     1501 gaggacgccg accacgttct tggtctcgat tcccggccga tcgatcacga tccg
//
LOCUS       DATAQL010000393         6477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_190870, whole genome shotgun sequence.
ACCESSION   DATAQL010000393 DATAQL010000000
VERSION     DATAQL010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190870"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1091..2566
                     /locus_tag="VHS97_06780"
     CDS             1091..2566
                     /locus_tag="VHS97_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3447940.1"
                     /translation="MATATKPNRKVAKSHQTKLLIDGKWRDSVSGETFATINPANEEV
                     IAQVAEGNAADIDLAVKAARKAFDSGPWRTMDARDRGRLMYKLADLIESHIDELAELE
                     TLDNGKPISESRHADLPLVIDCLRYYAGWSDKIHGQTIPIRGHYHCYTRREPVGVVGQ
                     IIPWNFPMLMVAWKWGPALAAGCTVVMKPAEQTPLSCLRMGELALEAGFPAGVINIVP
                     GYGETAGDALVKHPGVDKIAFTGSTEVGRLIMQNAAGTFKRVTLELGGKSPNIVFADA
                     DLDSAVNGAMLGLFLNQGQCCCAGSRLLVEDKVYNAMVERLAEAGEKRKLGDPFDAAT
                     EQGPQVDKTQFDKVLGYIAAGKHEGARCVTGGERFGSKGYFIKPTIFADVKDNMKIAT
                     EEIFGPVMQVLKFKDVDEVVKRANTTDYGLAAAVWSRDIKKAHTIAERLRAGTVWINC
                     YDVFDAAAPFGGYKASGIGRELGEKGLDNYTELKTVTVALD"
     gene            complement(3092..4729)
                     /gene="serA"
                     /locus_tag="VHS97_06785"
     CDS             complement(3092..4729)
                     /gene="serA"
                     /locus_tag="VHS97_06785"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX3447941.1"
                     /translation="MATYRVLVTDKLAEEGLAQLRADPGIEVVVDTKLAQNPVALRQA
                     LLEADGIAIRSGTQLTAEVLEGQSRLKAIVRAGVGVDNIDVAAASRRGIVVMNTPGGN
                     TISTAEHTMALLLSLARNVPKANDSLKSGKWERTKFTGSQLEGKTLGVIGLGRVGLAV
                     ARRAQGFGMSVLGFDPFLAADKALEYGVESVVRLDDIWGRCDFLTLHTPMTADTRNLI
                     GAREIALMKPSVRIINCARGGLIDEAALAEALNNGKVAGAAIDVFEPEPPPAGHPLVG
                     HPLVVVTPHLGASTEEAQVSVAVEAARLLSDFLSRGQVRFAVNMPALDKAELEDVRLY
                     LNLAWRLGMLHAQMDRGTLKNAKITYRGEVAHKNTKLITASFAAGLMETALEQQVNLV
                     NAIPLARERGIVIEERSADAPGDFATLIQTEVTTERKTYSASGTLFGKQFLRLVRLGD
                     YLLDAHIDGTLMIFTHMDQPGLIGFIGRTLGDEGVNIAQMNVGREEQGGEAIGVVNLD
                     SVPTTQALETLRQNPNVLSLSVINLPPLGAGPPWLGS"
     gene            complement(4815..5972)
                     /locus_tag="VHS97_06790"
     CDS             complement(4815..5972)
                     /locus_tag="VHS97_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX3447942.1"
                     /translation="MRKPRLMTPGPAMVPEDVLLELARPVIHHRSNEAKQVVAEVLEG
                     LKEVFQTKNDVLILTASGTGAMEAAVVNAVPPGGKALVLSAGYFGARWTNISKAFGIN
                     AVVLETEWGQPVDPDQVAEALRQHPDTAAVLGTLSETSTGTGHPVEAIGRIVAKTPAL
                     FAVDGISGVGAMECRTDQWGIDLLCVGSQKALMLPPGLAFVSVSPKAWTKIDTFDSHS
                     YYFNLKAARKKIKESDTPYTPAHTLILALRTALRRIKEEGLENVWQRHRKMSEACQAG
                     IQALGLELFSARPAEGLTAFRVPDGLKEGQIRNKLTERFGITTVGGQEKLKGTIIRIG
                     HMGYTDELDVISTLAALEMTLAELGYDVEPGRAITAAQQVLIGQKGAVAVG"
     gene            complement(6193..6417)
                     /locus_tag="VHS97_06795"
     CDS             complement(6193..6417)
                     /locus_tag="VHS97_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX3447943.1"
                     /translation="MNTDELSVFDLSPSEKLQLVEDLWDDLAATPEVVPVDEWQKDEL
                     ARRQANLLKNPASGLAWQEVERRVRSRHGR"
BASE COUNT         1302 a         1957 c         1949 g         1269 t
ORIGIN      
        1 gagttaaacg tttccgtgat cactcccggg accgtgctgg tctgcgctcc ggcagcctcg
       61 acggacagga cgattccggc atgggcctgc gtcgaaaact gcggagcaag cggcacaatg
      121 accgcgaagc atgctaaaac actcttggag atcttcatgt ataagaaaac cctttcgctt
      181 taatcgagta agaagagacg agtaaacgag ttggtttcaa aatcatgaac acaaagtgac
      241 gatttaaacg tttagattgt tgatgtgaca atcggtaacc gtcacgagaa gcttactaga
      301 gggttacgtc ggcgtgagtt ggtcgtttac ggaaggaaac ccgaggtgat tgctggcgac
      361 gtgatgtgag gttttgtcga gaatgtgatt gtggtcggcg tcaggacaac gcgtttgtgt
      421 gacgcttttc cattttcagc actattcccc gtcggacact tgattttgcg cgtcacggac
      481 ggggaactct gcgcatgcag cgctcgctga attcttgagg ttgatgccga cgggacctcg
      541 actgcgggag gaaatgatgg gtccttagcc gggcctgccc acagccactc gacttcgcgt
      601 ggcgccaggt taacggcgca agtcaacctg agccggcgct ctggctcggc cgcattctcg
      661 ccgtgatggt taggcgtggg ttgcggaggg tccggcaccg cccgctccag agagctcgcc
      721 gcggtgggag gttctagctc gtgaacagct tcgataattg gactgggccg gagacgtccg
      781 gccaggaagt catcccagac acgttgtcgc ccatctggcg gaacggctag taggcgttcg
      841 gccaccggcc ggaacatctc ggaaacccgc gacagttcat ctggcccgcg tcccaccacg
      901 cccccattgg caaggcgggc caaactctcg gattgctcgc aatgatcaat gatcgcggcg
      961 gaagttcagc cgaggatcgc cccaccgctt gagtgacact gggatcagcc ataagatggc
     1021 gccaggggaa cacacggaag aaatggacaa gtgcaaggaa cgcgcgtgct cgtgagaggg
     1081 agaacgaccg atggcaacag caaccaaacc gaatcgcaaa gtcgctaagt cgcatcagac
     1141 aaagctgctc atcgacggca agtggcgcga tagtgtcagc ggtgagactt tcgcgacgat
     1201 caatccggcc aacgaggaag tcatcgccca agtcgccgaa gggaatgccg ccgatatcga
     1261 cctggcggtg aaggctgccc gcaaggcgtt cgactcgggc ccatggcgca cgatggacgc
     1321 gcgggaccgc gggcgcttga tgtacaagct ggccgacctg atcgagtcac acatcgatga
     1381 gctcgctgag ctcgaaacgt tggacaacgg caagccgatc agtgagagcc gtcacgccga
     1441 cttgcccttg gtgatcgact gcctgcgcta ttacgccggt tggtcggaca agatccacgg
     1501 gcagacgatt ccgatcagag gtcactatca ctgctacacc cggcgcgagc cagtcggagt
     1561 ggtcgggcag attatcccgt ggaatttccc catgctgatg gttgcgtgga agtggggccc
     1621 ggcgcttgca gccgggtgca cggtcgtcat gaagccagcc gagcagaccc ccctctcctg
     1681 tctccgcatg ggcgagctgg cgctcgaggc aggatttccg gcgggcgtga tcaacatcgt
     1741 ccccggctat ggcgagactg ccggcgatgc gctggtcaag caccctggcg tcgacaagat
     1801 cgcgtttacc ggctcgaccg aggtaggccg gctcatcatg cagaatgccg cgggcacttt
     1861 caaacgagtg accctcgagc tgggcggcaa gagccccaac atcgtttttg cggacgccga
     1921 tctcgactcc gccgtcaatg gcgcgatgct tggtcttttc ctcaaccaag ggcagtgctg
     1981 ctgcgcgggt agccggctgt tagtcgagga caaggtctat aacgctatgg tcgagcggct
     2041 cgcggaagcg ggcgaaaagc gcaagcttgg cgacccgttc gatgcagcca ccgagcaagg
     2101 gccgcaggtc gacaaaaccc agttcgacaa ggtgctcggc tacatcgcgg cgggcaagca
     2161 cgaaggagcc cgctgcgtca cgggcggcga gcggtttggc tccaagggct acttcatcaa
     2221 accgacgatc ttcgccgatg tgaaggacaa catgaagatc gccaccgagg agatctttgg
     2281 cccggtgatg caggtcctga aattcaagga cgtcgacgaa gttgtgaagc gggccaacac
     2341 caccgactac ggcctcgcgg ccgccgtctg gtcccgtgac atcaagaagg cccacaccat
     2401 tgccgaacgc cttcgcgcgg ggacggtctg gatcaactgc tacgacgtct tcgatgccgc
     2461 cgcacccttt ggcggataca aggccagtgg catcggccgc gagctgggcg agaaagggct
     2521 cgacaactat accgagctca agaccgtgac agttgcgctg gattgacggc catagcgtcg
     2581 tccgctgggg gccgccgagg tgagtcatcg tgaagttctg ccttttgaaa cgaagacatc
     2641 gggctggaaa gcccgaccta cacgtccgcc gaggtgagtc attgtgaagt tctgcccttt
     2701 gatacgaaga cgtcgggctg gaaagcccga cctacacgtc cgccgaggtg agtcattgtg
     2761 aggacctgcc ctttcccccc cttgtgaagg gggggttggg ggggtggtca gcgagcgatc
     2821 agccacaggg agatcaaagg gcgtgttgat cctagggaga tcaaagggcg ggttggtcct
     2881 ggcgcaatcg caacagtgtg tccaacggct tctgagggcg gttccggcag aaacgaacgg
     2941 acttggaggc tgttcgaagg gccgatcgct tgccagcttt ttggaggcga catgcctgat
     3001 agcgccctga ccaccccccc aaaccccccc cttcacaagg gggggaaaat gatgttgata
     3061 tggaggtagc ttaagggaac cagggtaccc gtcacgaccc cagccaaggt ggcccagcac
     3121 cgagcggggg cagattgatc acgctcaggc tcagaacatt gggattttgt cgcaacgtct
     3181 ccagtgcctg agtggtgggt acgctgtcga ggttgaccac gccgatcgcc tcgccccctt
     3241 gttcctcgcg gccgacgttc atctgagcga tgttgacccc ttcgtctccg agcgtcctac
     3301 cgatgaagcc gatcagaccg ggctggtcca tgtgcgtgaa aatcatgagc gttccgtcga
     3361 tatgcgcgtc gagcaaatag tctcccagcc gcacaagcct aagaaactgc ttgccaaaga
     3421 gggtgcccga cgccgaatag gtcttgcgtt cggtcgtgac ctcggtctgt atcaaggtgg
     3481 cgaaatcgcc gggcgcatcc gcggatcgct cttcgatgac gattccgcgc tcacgcgcca
     3541 aaggtatcgc attgaccagg ttgacctgct gttcgagggc ggtttccatc agtcccgccg
     3601 cgaagctggc ggtgatcagc ttggtatttt tgtgcgcgac ctcgccgcga taggtgatct
     3661 tggcgttctt gagagtccct cggtccatct gagcgtgcag cattcccaac cgccaggcca
     3721 ggttcaagta gagccgcaca tcctcgagct cggccttatc gagcgccggc atattgacgg
     3781 cgaaccggac ctgaccgcga gacaagaagt cgctcaaaag ccgggcagct tcgacagcca
     3841 cggaaacttg ggcctcctcg gtggaggcac ccagatgagg agtaaccacg acgagcggat
     3901 gcccgactag ggggtgacca gccggcggcg gttcaggctc gaacacatcg atcgccgcgc
     3961 cggcgacctt gccattgttg agcgcctcgg ccaaggccgc ttcgtcgatc agtccgccgc
     4021 gcgcacagtt gatgatccgg actgagggtt tcataagcgc gatctcgcgc gcgccgatca
     4081 ggttgcgcgt atcggctgtc atcggcgtgt gcaacgtcaa gaagtcgcac cgaccccaga
     4141 tgtcgtcgag tcgcaccacg ctctcgacgc cgtattcgag agccttgtcg gccgccaaga
     4201 atgggtcgaa gccgaggacg ctcatgccga atccttgcgc ccgtctggcg accgccaacc
     4261 cgacccgtcc cagtccgatg acccccagtg tcttgccttc gagctgactg ccggtgaact
     4321 tggttcgttc ccactttccc gacttgaggc tgtcgttggc cttgggcacg tttcgcgcca
     4381 gggacaagag caaggccatg gtatgttcgg ccgtcgagat cgtgtttccc cccggcgtgt
     4441 tcatcacgac gattccgcgc cggctcgcgg ccgcgacgtc gatattatcg acgcccacac
     4501 ccgcccgcac gatcgccttc aacctcgact ggccttccaa gacctcggcc gtcagttgcg
     4561 tgccggaccg gatcgcgatt ccgtcggcct cgagaagcgc ctggcgcagg gcaaccggat
     4621 tttgggccag cttggtgtcg accacgacct cgattcccgg atccgcgcgg agctgcgcga
     4681 gaccttcttc agccagcttg tccgtgacca atacacgata ggtcgccatc caagaccctc
     4741 cacgccgacc ctcccctaca actggggagg gagggagaac tcacattgtc cgaataaact
     4801 tgatccacct gtcctcagcc gaccgcgacc gcgcctttct ggccgattaa aacctgctgc
     4861 gcggcggtga tggcccgacc gggctcgacg tcgtagccca attcggcgag agtcatttcc
     4921 agggccgcca gggtgctgat cacgtccagt tcgtcggtat agcccatgtg gccgatccga
     4981 atgatcgttc ccttcagctt ctcttgacct ccgacggtcg tgatgccgaa acgctcggtt
     5041 agcttgttgc gaatctgacc ttccttcaag ccgtcgggaa cgcggaaggc cgttaatccc
     5101 tctgccggtc tcgcgctgaa aagctcgagc ccgagcgcct gaattccggc ctgacaagcc
     5161 tcgctcattt tgcggtggcg ctgccagacg ttttcgaggc cctcttcctt gattcgccgc
     5221 aaggcagtgc gcagcgcgag tatcagtgtg tgagccgggg tatagggcgt gtcggactcc
     5281 ttgattttct tccgcgccgc cttgaggttg aagtaatagc tgtgcgagtc gaacgtgtcg
     5341 atcttggtcc acgccttcgg gctcaccgac acaaacgcca ggcccggcgg caacatcaag
     5401 gctttctgtg agcccacgca gagcaggtcg atgccccatt ggtctgtccg gcactccatg
     5461 gcgccgacgc ccgagatccc atcgaccgca aagagagcgg gcgtcttcgc gacgatccgg
     5521 ccaatcgcct cgacgggatg gccggtcccc gtcgacgtct cgctcaaagt acccagcacg
     5581 gcggccgtgt caggatgttg acgcagcgct tcggccacct ggtctggatc aacgggctga
     5641 ccccactccg tctcgagcac cacggcgttg ataccgaacg ccttactgat attggtccag
     5701 cgcgcaccaa agtagcccgc gctcaagacc agggctttgc cgccgggagg caccgcgttg
     5761 accactgccg cttccatcgc gccggttccc gaagccgtca ggatcaagac gtcatttttc
     5821 gtctggaaga cttctttcag tccttcaagt acctcggcga cgacctgttt ggcctcgttc
     5881 gagcggtggt gaatcaccgg ccgagccagc tccaagagca cgtcttcggg aaccatggcg
     5941 ggtccgggag tcatcaatcg tggtttacgc aatttttgtt tccttcaaag aataactctt
     6001 tgcattctgg atcgaccgat cacagaaatc ggcgcccgaa tgaactatca tttactaata
     6061 aatgcctata cgtaggcaaa cgctccaggt cactcaagga gccgttgaca ccatttttcc
     6121 ggatctcgcg atgtgtggaa gacacaataa actgtgacta atgcatttgc atattcaggt
     6181 gccacaatca gttcagcggc catggcggct ccgcacccgt cgctcgacct cctgccaggc
     6241 cagacccgag gcaggattct ttaacaggtt ggcctgccgc cgagcaagtt catccttctg
     6301 ccattcgtca accggcacca cctcgggtgt ggccgcaagg tcgtcccaga gatcctcgac
     6361 gagttgaagc ttctctgagg ggctcaggtc gaaaaccgag agctcatctg tgttcatagg
     6421 aacggctcca agaccaaatc gcatcgaatg tcgtgcgcct cctgatgcac cgtacgc
//
LOCUS       DATAQL010000394         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2634600, whole genome shotgun sequence.
ACCESSION   DATAQL010000394 DATAQL010000000
VERSION     DATAQL010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634600"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(428..2002)
                     /locus_tag="VHS97_06800"
     CDS             complement(428..2002)
                     /locus_tag="VHS97_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3447944.1"
                     /translation="MAVKSKSKSQRKAQPHALHKPRGVIHPRVQAVGPEHFAFLCVDC
                     AKARSKMMLADFYGRVVLEPTTVEHNKAGFDNASKAVRDAMAQHHIKDVIVVVERTGR
                     YHGPIQRAFRETGFEVRVVHPFTTKQYRLPADPGNKTDDTDLSAIHRAAVNGFGLSEH
                     EPDPVSVQLQLLARHRRKLVRKRVVLQQQMLEHLQSFMPGYSKCFADVFDSPIVLWGA
                     TNLGSAAAILEAGVDGLTEQVRRAGIRGHQPTVEKIVAWARSAPSAEEPASLHQRIFV
                     ELEADRLAKAQSIEAVERDLAGWLARTPYMLLLSIVGINVVSAAEFAGEMGPIERYTK
                     ARAITGRAGLFPSRYQSDEVDRRNGTLVRHANHSLRYAILMIADNLIRCNEHFRVLAA
                     GWRLQGKDAHALRIQAAGRFCRIAYYMVAGRNTFQHPSAQRRDYILDKLIRFSLEHST
                     EPDQILRNLDATVAQLPRHAHREEAVPLAEELARVRKPRGAGPKVLRTILPAVLAKLE
                     VELVRSNESGEPDPTE"
     gene            2304..>2523
                     /locus_tag="VHS97_06805"
     CDS             2304..>2523
                     /locus_tag="VHS97_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447945.1"
                     /translation="MNPLRRHVLLLFRTLALAPTLVVLCSSQARAQMGYMGGFGYPGM
                     GYGYPMMGGYGFGGPGFGYGAAGMGGFGY"
BASE COUNT          456 a          732 c          822 g          513 t
ORIGIN      
        1 cctactccgt ggctctgctg agtttattgt agcttcggag cctgctccgt ggagaaagac
       61 tgagcgagaa atcagagcca gttacgatcg gaagaaacat tgtaggcttg acgcggctcg
      121 gggttctcgt tcgagaaaga aagacagcca taggggagtt gagcggagcg gctggcgtga
      181 tcaatgtacg gacgttacct agccccgccg atttttgcgg gtttgacaac tgtcgttcat
      241 gacggtcatc gccaagcagc cacctctgtc tcgtccagga tcacgtctcg ccatactggc
      301 acatcagggg agtgacccca cacagcctta tcagacgttg gtgatccgag acagagcgcc
      361 acgcttttcg tcacggcttt gcgccattgg acatgctacg acgttgtccg gctcctcagc
      421 gcgacagtca ctctgtggga tcgggctccc ctgattcgtt cgatcttacc agttcgacct
      481 ccagtttggc caagacggcg gggagaatcg tgcgcaggac cttcggtccg gccccccgcg
      541 gtttccggac ccgagccagt tcctccgcca gagggaccgc ttcctcacgg tgcgcgtgac
      601 ggggaagctg agccaccgtg gcgtcgaggt tccgcaggat ctgatcaggt tcagttgaat
      661 gttcaagaga aaacctgatc agcttatcaa gaatgtaatc cctccgctgg gctgaggggt
      721 gttgaaaggt gtttcggccc gcgaccatgt agtaggcgat ccggcagaac cgaccggccg
      781 cctggatgcg aagcgcgtgc gcgtccttcc cctggagcct ccagccggcg gccaggactc
      841 ggaaatgctc attacatcga atcaggttat cggcaatcat caagatcgcg taccgcaggc
      901 tgtggttggc atgtcgaacc agcgtgccgt tgcggcgatc gacctcgtcg ctctggtagc
      961 gcgacgggaa caacccggcc cggccggtga tcgctcgggc cttggtgtaa cgctcgatcg
     1021 gccccatctc gccagcgaac tcggcggccg aaacgacgtt gatgccgacg atactcaaga
     1081 gcaacatgta gggcgtgcgt gccaaccatc cggccaggtc gcgctcgacg gcttcgatgc
     1141 tctgagcctt tgcgagccgg tccgcctcga gttcgacgaa aatccgctga tggagtgacg
     1201 ccggctcctc agccgaggga gcggaccgcg cccaggcgac gatcttctcg accgtgggct
     1261 ggtggcctcg gatgccagct cgtcgaacct gctcggtcaa gccgtcaacg ccagcttcga
     1321 ggatcgccgc ggccgagccc aggttcgtgg cgccccagag aacgattgga gaatcaaaaa
     1381 catcagcgaa acattttgag tatcctggca tgaacgactg aagatgttct agcatttgct
     1441 gctggagaac cacgcgcttt cgcacgagct tgcggcggtg cctcgccagc agttggagtt
     1501 ggacagagac cgggtcgggt tcgtgctccg agaggccgaa gccgttgact gccgcccgat
     1561 ggatcgcgga gaggtccgtg tcgtcggtct tgttgccggg atcggcgggg agtcgatatt
     1621 gcttggtggt gaacgggtgg acgacgcgga cctcgaatcc cgtctcgcgg aaggcgcgct
     1681 ggatcggtcc gtgatagcgg cccgtgcgct cgacgacgac gatcacgtcc ttgatgtggt
     1741 gttgggccat ggcgtcgcgg acggccttcg aggcgttgtc gaagccggcc ttgttgtgct
     1801 cgaccgtggt gggttcaagg acgactcgcc cgtagaaatc ggcgagcatc attttggagc
     1861 gggccttggc gcaatcgacg cacaagaacg cgaaatgttc cggcccaacc gcctggacgc
     1921 gtgggtgaat cacacctcga ggcttgtgaa gggcatgcgg ctgagccttg cgctgactct
     1981 tcgacttgga tttgacggcc attggacaga acctccgaat cgagttgaga agactcaact
     2041 cccattctgg aagtctgttc gggcgggtgg gtctaaagga tttagaccgc ttgttgctgc
     2101 tggtcagaat cctttagtac tttaattcgc cacatgccag gacatgcaat gaagatggtc
     2161 gtgcgccccg ccgcgcaaat ctccgcgagc gagttcgacg ccgacgccgc gctggctcta
     2221 caatagggga agccgagccg gcgggttggt gtggcctacc ggcggcggga tcgcttggtc
     2281 cggccaggtt tgggaggctc gcaatgaacc cgctccgacg ccacgtcttg ctgttgtttc
     2341 ggacgctggc cctcgcaccg acgctcgtcg tcctctgcag ctcgcaggcc cgcgcccaga
     2401 tgggctacat gggtggcttt ggctacccgg ggatgggcta cgggtatccg atgatggggg
     2461 gctacggctt tggcgggccc ggcttcgggt acggcgcggc cggtatgggt ggcttcggct
     2521 acg
//
LOCUS       DATAQL010000395         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3245906, whole genome shotgun sequence.
ACCESSION   DATAQL010000395 DATAQL010000000
VERSION     DATAQL010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3245906"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VHS97_06810"
     CDS             <1..225
                     /locus_tag="VHS97_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447946.1"
                     /translation="GTAGGGSVPYANTFTPSPYPEIGTRVYDRYSSYEPTDWHAEWHK
                     TLYSFVWGHDPDVSTAREIEASVYGFQSDH"
     gene            350..649
                     /locus_tag="VHS97_06815"
     CDS             350..649
                     /locus_tag="VHS97_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447947.1"
                     /translation="MGRTRYRFGGATFPHFLTCTVVGWLPVFTRPEIVQFVLDSWQFL
                     QDQDRLVLFGFVVLENHIHFIGCRHNRQVTQRLETTRSVDDGIPTPSVGTSQTFD"
     gene            1077..1739
                     /locus_tag="VHS97_06820"
     CDS             1077..1739
                     /locus_tag="VHS97_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447948.1"
                     /translation="MQGTVLMLMALSGLGCHNKSCDVAYAPPTYSCFGGGCYANVYPT
                     DYASPGTYSGCYSGCYSGCYSNSWGGCYSSCFSGCFGGYGGGHGCGLLARLFGGCGRR
                     HHSYSYGGCYGGGCYGGGCYGGGLGYDMPYEPPIFGYALQYNYGTMGTNQYPSAPPGA
                     TATPVPSEAAPPVPPNPAPSTPTTPSTPTPAAPMPETPAPVTPPPPVPADVTPPAPKP
                     GA"
     gene            2081..2968
                     /locus_tag="VHS97_06825"
     CDS             2081..2968
                     /locus_tag="VHS97_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3447949.1"
                     /translation="MDARYSGPQSTVGSNDSGLSATMPEFTDDAERRDWLLKTLGKTF
                     CRKLGLFRIPETLTLSVVIPVYNERKTIHEILRRVRAVPVKKQIVVVDDCSTDGTRDI
                     LKEIEKRDGDLTVVYHVINQGKGAALRTGFREASGEIVIVQDADLEYDPEQYPQLIQP
                     IVEDIADVVYGSRFIGETHRVLYFWHSLANKFLTLLSNMFTNLNLTDMEVCYKVFRRE
                     VIQGIKLKSDRFGFEPEVTAKVARFTFPPDNGQPPQPCRIYEIPVSYHGRTYREGKKI
                     GLKDGFQALYCIVRFAIAD"
     gene            2984..>3697
                     /locus_tag="VHS97_06830"
     CDS             2984..>3697
                     /locus_tag="VHS97_06830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3447950.1"
                     /translation="MFQGVDKRACATAVAMFAILASAGLAMGRQVAGTTQSAPDPTSK
                     SQTGARQDPAKSPPKPKTDRKSKTRVRRDAPGFYMGRQIAQVMSWEEFQWLFRETRVE
                     EEQPEAMLNALDIPRGATVADVGAGAGYHSIRLARRVGNKGTVLATDIQPEMIRMLQE
                     NARNAGVGNIKSIRATQSDPKLPESKVDLILMVDVYHECSDPETILLGLLKALKPRGR
                     LVLVEFRAEDPEVPIRPEHK"
BASE COUNT          732 a         1116 c         1071 g          778 t
ORIGIN      
        1 ggaaccgccg gcggcggctc cgtgccgtat gccaacactt tcacgccgtc gccatatcca
       61 gaaatcggca cacgtgttta cgaccgctac tcttcgtatg aaccgaccga ctggcacgcc
      121 gaatggcaca agacgctcta cagttttgtg tggggccacg atccggatgt cagtacagcg
      181 cgcgagatag aagcttcggt ttacggcttt caatcagacc actagtgaac cgtcaaactt
      241 cgatcgacag gtggtctgct cgcactcgtt cccactcgtt cccacgctcg gcgtgggaat
      301 gccgtctgcg acgctccgcg tagtcttctc gagccaagaa ccacgactca tgggacgcac
      361 ccgttatcgg ttcggaggag ccactttccc ccacttcctg acctgcacgg tcgtcggctg
      421 gctgcccgtc ttcactcggc ccgagatcgt tcaattcgtg ctcgattcgt ggcagtttct
      481 ccaggatcaa gatcgcctgg tgctgttcgg atttgtcgtc ctcgagaacc acattcactt
      541 catcggttgc cgtcacaacc gacaggtgac ccagcggttg gagacgacgc ggagcgtcga
      601 cgacggcatt cccacgccga gcgtgggaac gagtcaaacc ttcgattgac gggtggtctt
      661 tccgaagggc taatgacgca aagaatgcga actgaaatga caacgacccc ggcgggttga
      721 cctgtcaatc gaggttgacg ccacgttgag gcttcgagga agaggttttt gctctcagac
      781 cgaaaagtga cggggatagc gtaccccgtt cagcccgtag attgcccagt cttgtttttc
      841 ccactatttt cacttttcga gattgaaatg gggtatgatg gtggttagag tctaggaggt
      901 ctgagcagag aataggaatt cgggcgactt gacgggtatt cgaggcgtgg gtttctcggg
      961 agagaacgag cgtctcgcgg ggatgcccga ccgctttggg gttgcaccac gggccatcat
     1021 cggtgccggt cgcgggttga ttgattctcg tcgatttcgc acctgggaga aatgcaatgc
     1081 agggaacagt gttgatgttg atggccctca gtggtttggg ctgtcataac aagagctgcg
     1141 acgtggccta cgcgccgccc acttacagtt gcttcggtgg tggttgttac gcgaacgtct
     1201 atcctaccga ttacgcttct ccgggaacgt actccggctg ctactcaggt tgctattccg
     1261 gctgctattc caactcttgg ggtggctgct attcgtcatg tttctcaggc tgcttcggcg
     1321 gctatggtgg aggtcacggg tgcggcctcc tggccaggct atttggcgga tgcggccgga
     1381 ggcatcactc ctacagttat ggcggttgtt acggcggcgg ttgttacggc ggcggttgtt
     1441 acggcggtgg gttgggctat gatatgccct atgagccgcc gattttcggc tatgctctgc
     1501 aatacaatta tggcacgatg ggcacgaatc aatacccatc ggcccctccg ggcgcgacgg
     1561 caacccccgt accgtccgag gcggctccgc cggtcccgcc caatccggcg ccgagcacgc
     1621 caacaacgcc gagcacgcca acgccggctg cgccaatgcc tgagacaccc gctccggtga
     1681 ccccgccacc acctgttccg gctgacgtca cgcctccggc acccaagccg ggtgcctgat
     1741 tcgcgaaccg cggggtggct cgaatcccca gccgggggcg actggggact ttcgcgaggt
     1801 cactgcccct gcgaactgaa tatgcgtaat cccaagccga gtctcacccg taacgaagcg
     1861 gcagtcgacc gctcgatgcg gatgagattc ggctttcgtt ttggtcgcgg ttcctcgcca
     1921 acccaggtgc acgatcacaa cgtggcttcc atccggtggt ttcctcgctt atcttgatct
     1981 aagagatttt tcggcgaagc gatcgctgcg tcatgctcgt ctcggccgat caagcggcgc
     2041 ctctggtcaa tccacatgat attcggagtc tctcctggcg atggatgcgc gctactctgg
     2101 tccccaatca acggtcgggt cgaacgactc tggtctgagt gccacgatgc ctgagtttac
     2161 cgatgacgcc gagcgcaggg attggctctt aaaaaccctc ggtaaaacgt tttgccgaaa
     2221 gctcggtctc ttccggattc cggaaacgtt gacgctttcg gttgtcatcc ccgtttacaa
     2281 cgaacggaag acaatccacg agatcctccg gcgtgtacgt gcggttcccg tcaagaaaca
     2341 gatcgtcgtt gtcgacgact gctccaccga cggcacacgc gatattctca aagagatcga
     2401 aaagcgggac ggcgatctga cggtcgtcta tcacgtgatc aaccagggca aaggagccgc
     2461 tctgcgtacc ggctttcggg aggccagtgg cgagatcgtc attgtccagg acgcggacct
     2521 cgaatacgac cctgagcagt atccccagct gattcagccg atcgtcgagg acattgccga
     2581 cgtcgtttac ggctcgcgct tcatcggcga aacgcatcgc gtgctctact tctggcattc
     2641 actggccaac aagtttctca cgcttctctc caacatgttc accaacctta acctgaccga
     2701 catggaggtc tgttacaagg tgtttcgccg tgaagtgatc cagggaatca agctcaagag
     2761 tgaccggttt ggcttcgagc cggaggttac tgccaaggtg gcccgcttca cttttccgcc
     2821 agacaatggt cagcctccgc agccgtgccg catttatgaa atccccgttt cctatcacgg
     2881 caggacttac cgcgagggca aaaagatcgg cctgaaagac ggcttccagg ctctctactg
     2941 catcgtccgc ttcgcaatcg cagactgagg tgaggagccg gccatgttcc agggcgtgga
     3001 caaaagagcc tgcgcgacgg cggttgcgat gttcgccatc ctggcaagtg ccggtctcgc
     3061 gatggggcgc caggtggccg gcaccacgca gtcggcgccc gacccgacgt ccaaatccca
     3121 gacaggcgcc cggcaagatc cggccaagtc accacccaaa cctaagaccg accgtaagtc
     3181 gaagacgcgc gtccgacgcg acgcgcccgg tttctacatg gggcgacaga tcgcccaggt
     3241 catgtcgtgg gaggagtttc aatggctctt ccgcgagacg cgagtcgagg aagaacagcc
     3301 cgaagcgatg ctcaatgcgc tcgacattcc gcgcggcgcg accgtcgccg atgtcggtgc
     3361 gggggcaggc taccatagca ttcggctcgc ccgccgggtt ggcaacaaag ggaccgttct
     3421 ggccaccgat atccaacccg aaatgatccg gatgcttcag gaaaacgccc ggaatgccgg
     3481 cgtcggcaac attaaatcga ttcgcgcgac ccagtccgat ccaaagctcc cagaatccaa
     3541 agtcgacctg atcttgatgg tcgatgtcta ccacgagtgc tccgaccctg aaaccatcct
     3601 tctgggactc ttgaaggccc tcaagccccg aggccgcctc gtgctggtcg agtttcgcgc
     3661 ggaagacccc gaagtgccga tcaggcccga acacaag
//
LOCUS       DATAQL010000396         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3091882, whole genome shotgun sequence.
ACCESSION   DATAQL010000396 DATAQL010000000
VERSION     DATAQL010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3091882"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VHS97_06835"
     CDS             complement(<1..816)
                     /locus_tag="VHS97_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447951.1"
                     /translation="MILIVQAALVLAVSLAVRSAVVPLGVPGEWEWLRVKVAPPWDGL
                     ALAGLSVAAYAGFAGLGFKALGRNPLPRVEMTWLAGLLAAAIAIQIVVPMGAASGYDL
                     SKWAAVNYLPGSTGYFKIARQQAIRDPWKFMADYSDWIRNQDSLHIGTHPPGLIAAQC
                     VLIKEMERNPGLADFLIDHMPASTAMGFRVFGGNDPQPLSRSDRAALYSTAILTLLAC
                     AGTVVPLYLLTRVALSAPAAWAAAALWPLAPAANLFQPVADTAYPFLSTTAVAL"
     gene            1045..2451
                     /locus_tag="VHS97_06840"
     CDS             1045..2451
                     /locus_tag="VHS97_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3447952.1"
                     /translation="MHFEDASAPQIPAGQTNLPWWKDLTRYQWFVFVVASLGWLFDTM
                     DQQLFNLARKPAVTELLAAGSTSPPSSGAVAENAGFATMIFMIGWALGGIFFGILGDR
                     IGRAKTMMLTVLCYSAFTGLSALSKGVLDFAVYRFLTGLGVGGQFAVGVALVAEVMSD
                     RARPFALGLLQALSAVGNMMAAVASIALGKLEEAGTIGSAWRAMFLVGLAPAVLAIPI
                     FLRLKEPERWKAVARDEEIAGSRKYKLGSLAELFGDPRWRKNTIVGMVLAFSGVVGLW
                     GIGFFSFDLIRAVFVEHFRAQGLSPQEVAGKLTFWSGITSFVQNAGAFLGIYAFTRVT
                     HHIGRKPAFAISFVVAAIATAATFWFLDSFWQIFVLIPIMGFCQLALFGGYAIYFPEL
                     FPTRLRSTGTSFCYNVGRLVAAAGPLTLGLLTSKVFAGYPDPMPSRLAGVAMCSVFLL
                     GLLALPFAPETKGQPLPE"
BASE COUNT          500 a          830 c          798 g          536 t
ORIGIN      
        1 cagggccacg gcagtcgtcg acaggaacgg ataggcggta tcggcgaccg gctgaaacag
       61 gttggccgcc ggtgccaagg gccaaagtgc cgccgccgcc catgccgccg gcgcggagag
      121 cgccacgcgg gtcagcaggt agagaggtac gaccgtcccg gcgcaagcga gtagagtgag
      181 aatcgccgtc gaatataggg ccgcccggtc agaccgcgag agcggttgcg gatcattgcc
      241 tccgaacacg cgaaagccca tcgcggtgga cgccggcatg tggtcgatga gaaaatcggc
      301 caagcccgga tttcgttcca tttccttgat caaaacgcac tgagcggcga tcaagcccgg
      361 cgggtgggtg ccgatatgga gcgaatcctg gttgcggatc cagtcggaat agtccgccat
      421 gaatttccag ggatcgcgaa tcgcctgctg gcgagcaatc ttgaaataac cagtcgagcc
      481 aggcagataa ttgaccgcgg cccacttgct caggtcatag cccgacgccg cacccatggg
      541 tacgacgatc tggatcgcga tcgccgctgc taagaggccg gcgagccagg tcatttccac
      601 ccgtggcaag ggattccggc cgagcgcctt gaatcccagt cctgcaaagc ctgcatacgc
      661 ggcgacactc agaccggcga gcgctagccc gtcccacggg ggcgcaactt tcacgcgcaa
      721 ccactcccat tcgccaggca ctcccagcgg caccacagca ctcctcacgg ctagcgacac
      781 cgccaaaaca agtgcggcct gaacaatcag gatcatccaa gtcatcgcgc gcataccctc
      841 gatattagcc ctcggtcgga gacccggcga gggatcttct cgctgtaaaa ggacgtctcg
      901 attgcgatcg gcgccggtcc atgttctgat cgtggctaat tatgcactgt tagctttctg
      961 ctgattcatc gacattgtga ctaatccagc gcctcacccc tcagcgatcg tcacgcagtc
     1021 actcagccaa cggagacttc ctagatgcat ttcgaggacg catccgcgcc ccaaatcccc
     1081 gcgggccaga ccaacctccc ctggtggaaa gatttgacgc gataccagtg gttcgtgttc
     1141 gtagtcgcct cattgggctg gctctttgac acgatggatc agcagttatt caatctggcc
     1201 cgcaaacccg ccgtcaccga gttgctggct gccggatcga cctcgcctcc gagttcgggc
     1261 gcggtcgcgg aaaacgcagg cttcgccacc atgatcttca tgattggctg ggctttgggc
     1321 ggcatctttt tcggcatcct cggcgaccgg atcggccggg ccaagacgat gatgttgacc
     1381 gtcctttgct attcggcttt cacaggtttg agtgcgctgt cgaaaggcgt cttggacttc
     1441 gctgtttaca ggttcctgac cggactcggg gtaggagggc aattcgccgt gggtgtggcc
     1501 ttggtcgcgg aggtcatgtc ggatcgcgca cggccgttcg cgctggggtt gcttcaggcc
     1561 ctctcagccg tcggcaatat gatggcggcc gtggccagta tcgcactggg gaagctcgag
     1621 gaagccggca cgatcggcag cgcatggcgg gccatgtttc tggtcggcct ggctccggcc
     1681 gtgctcgcca ttccaatctt tctccgctta aaggagcccg agcgctggaa ggccgtcgcc
     1741 agggatgaag agatcgccgg ttcacggaag tataaactgg gctctctcgc cgaactcttc
     1801 ggcgatccgc gctggcggaa aaacacgatc gtcggcatgg tgctagcctt ctcaggagtg
     1861 gtgggtcttt ggggaatcgg ttttttcagc ttcgacctga tccgagcggt ctttgtcgag
     1921 catttccgtg cccaagggct cagcccccag gaggtcgccg gcaagctcac gttttggagc
     1981 ggaatcacct cgttcgtgca gaatgcaggc gcgttcctgg gtatctacgc cttcacgcgc
     2041 gtcacccacc acattggcag aaaaccagcg ttcgcgatct ccttcgtcgt ggccgcgatc
     2101 gccacggccg cgaccttctg gttcctcgat tcgttctggc agatctttgt cttgattccg
     2161 atcatgggtt tctgccagct cgcgcttttc ggcggttatg ccatctactt ccctgaactg
     2221 tttccaaccc gactgcggag taccggcaca tcgttttgct acaacgtagg ccggctcgtc
     2281 gccgcggcag gtccgctgac cctgggcctc ctgaccagca aagtcttcgc cggctatccc
     2341 gatccaatgc cgtcgcggct ggccggggtc gcgatgtgct cggtatttct cctgggcttg
     2401 ctcgctctcc ccttcgcacc cgagaccaaa ggccagccgc tgccggaatg acatggtcga
     2461 agtgacgcac gaccatgttg gaagcgtatc gagcgcaggg aatccccgca gcttgattaa
     2521 agaattgtcg atctgacgca acgctgccga aagtcagagc accagccggg ccgactcacg
     2581 atcgaggtaa agccttgccc ccgaatgacg gcgcagcgcc gtggcggggc atgcctcact
     2641 gatcggacca cgcaatgcgg tcaa
//
LOCUS       DATAQL010000397         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_497770, whole genome shotgun sequence.
ACCESSION   DATAQL010000397 DATAQL010000000
VERSION     DATAQL010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497770"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VHS97_06845"
     CDS             <1..608
                     /locus_tag="VHS97_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447953.1"
                     /translation="RQGNGRVERGGLESQDGGPQGHRARAGSEGKAAVADATSPSRVR
                     DMMTELSQRPGWTPEEAQAWARALGQVGVANALHMPNNPPGFSPPANTDRPSTLIRGP
                     NAHLLTPEQEAQVQELARQSMAGAEGDEFSHDMTHIDEFLARGHPAYQPMPMTPPGTT
                     FGPREVAGPDMVGQTPYAPSSQRAAHYGKDAVERELDKEPE"
     gene            609..>1843
                     /locus_tag="VHS97_06850"
     CDS             609..>1843
                     /locus_tag="VHS97_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447954.1"
                     /translation="MALKIPSNPDLMRAWVKEMIDECMASASERGMVYTRAAQYYYTG
                     AADSRAALYNKTKSFIDKLAGFLMQPTDVRLRLIYDSGEPEDVLERSELVGEKLSADF
                     RQVDADVTFADAVTWSLINGCGLLKVLPDGDSGTFKVAQVHPQNFGVLSETTLHLDEQ
                     EAFCHVSYPSKSRLRTSLIDHPRYNEIMKALDEDPGPDRDEEESTYFHQMVVGGLQPL
                     GDVGDAPSSAAGIVNVFPVPTPWRPNRRFAPTVKHCELWVKDRDRDEDWTTIQVLYGH
                     DPIVIEGDDTRRNLSRVPGKNAFVKVQGHETPGYFWGRSSIADVQMLQDMLNKRLRDI
                     KIMWDRNVNAPQVFSGFTSVTEEQYFKIVNEGGFINDPNPNAKASKLLDPPPENYLEE
                     LEFVFKLFDEAAGFSPIMSG"
BASE COUNT          403 a          574 c          569 g          297 t
ORIGIN      
        1 gtcgacaagg aaatggacgc gtcgaacgag gaggcttgga aagccaagat ggcgggccgc
       61 aaggccaccg ggcgcgggcc ggatcggaag gcaaggcggc cgtcgccgac gccacgagcc
      121 cgtcgcgcgt tcgcgacatg atgacggaac tgtcgcagcg gccgggatgg acgcccgagg
      181 aagcccaagc ttgggcgcgg gcgcttgggc aagtcggcgt cgcgaacgct cttcacatgc
      241 cgaacaaccc gccgggcttc agtccgcccg cgaacaccga ccgtccgtca accctcattc
      301 gagggccgaa cgcgcacctt ttgacgcccg agcaagaggc gcaggtgcaa gagctggcgc
      361 ggcaatcgat ggcgggcgcg gagggcgacg agttcagcca cgacatgact cacatcgatg
      421 agtttctggc gaggggccac cccgcctatc aaccgatgcc gatgacgccg ccggggacga
      481 cattcgggcc gcgcgaagtg gccggacccg acatggtggg ccagaccccc tatgcgccat
      541 catcgcaacg cgcggcgcac tacggcaagg acgcggtgga gcgcgaactc gacaaagagc
      601 ccgaatagat ggcgctcaag atcccgtcca accccgacct catgcgggca tgggtcaagg
      661 agatgatcga cgagtgcatg gcttcggcca gcgagcgcgg gatggtctac acccgagccg
      721 cccaatatta ttataccggg gcggccgaca gccgcgccgc cctctacaac aaaaccaaat
      781 ccttcatcga caagctcgct ggcttcctca tgcagccgac cgatgtccgg ctgcgcctga
      841 tctacgacag cggcgagccc gaggatgtcc tcgaacgctc cgagctggtg ggcgagaagc
      901 tgtcggccga cttccgccaa gtcgacgccg acgtgacctt cgccgacgcc gtcacatgga
      961 gcctgatcaa tggctgcggc ctgttaaagg ttctgcctga cggggacagc ggcacgttca
     1021 aggtggcgca ggtccacccg cagaattttg gcgtcctatc cgagacgacg ctgcatctcg
     1081 acgagcaaga ggcgttctgc cacgtcagct atcccagcaa gtcgcggctg cgcacgtcgc
     1141 tcatcgatca tccgcgctac aacgaaatca tgaaggcgct cgacgaagat ccggggccag
     1201 accgcgacga agaggaatcg acctattttc accaaatggt tgtaggcggg ctgcaaccgt
     1261 tgggagacgt tggcgacgca ccgtcatccg cagccgggat cgtcaacgtc tttcccgtcc
     1321 cgacgccgtg gcgtcccaac cggcgattcg ctccgaccgt caagcactgc gaactatggg
     1381 tgaaggatcg cgaccgcgat gaggactgga cgacaatcca agtgctttac ggtcacgacc
     1441 cgatcgtcat agaaggcgat gacacccggc gcaatctgtc acgcgtcccc ggcaagaacg
     1501 cgttcgtcaa ggtgcagggg cacgagacgc cgggctactt ctggggccgc tccagcatcg
     1561 ccgacgtgca gatgttgcag gacatgctga acaagcggct gcgcgacatc aaaataatgt
     1621 gggatcgcaa tgtcaatgcc cctcaagtct tcagcggctt caccagcgtt accgaggagc
     1681 aatacttcaa gattgtcaat gagggaggtt ttattaacga cccaaatccc aatgcgaaag
     1741 cgtcgaagtt actggacccc ccaccggaaa actacttgga agaacttgag tttgttttca
     1801 agttgtttga tgaagctgct ggcttcagcc cgatcatgtc agg
//
LOCUS       DATAQL010000398         3810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_649949, whole genome shotgun sequence.
ACCESSION   DATAQL010000398 DATAQL010000000
VERSION     DATAQL010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649949"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VHS97_06855"
     CDS             <1..750
                     /locus_tag="VHS97_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX3447955.1"
                     /translation="KPPNAEVPPTNWLLSTGELKWCGHNGQGFAFDNEGPRHRVYLEP
                     FQISSRPVTSGEYVAFIEDGGYDRPELWLSDGWNAVKTHRWKAPLYWEQVAGAWWVMT
                     LGGFRRLTDAEPTCHVSYYEADAFARWAGARLPTEFEWESAAIEQEVNGNFLESGDLH
                     PQPALPGAEVASLFGDIWVWTQSPYVPYPGARPTAGALGEYNAKFMCNQLVLRGGSCA
                     TPASHIRATYRNFFPPEARWQFTGIRLAKDA"
     gene            747..1718
                     /gene="egtD"
                     /locus_tag="VHS97_06860"
     CDS             747..1718
                     /gene="egtD"
                     /locus_tag="VHS97_06860"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX3447956.1"
                     /translation="MISTSLAIVRHNETLSRRRQFLKDVIQGLSRPQKEIACKYFYDE
                     VGSALFDEICELDEYYLTRTEMAILEAHAAEMADAIGEDCELIELGSGNAQKTRLLLE
                     QLRSPRAYLPVDISREPLELSALRLAKQFPELRVLPVHGDFTGPLLLPETGDPRARRV
                     VYFPGSTIGNFSPRAATSLLCTIAWLVGDGGGLLIGFDLDKDPHIVWPAYNDSRGVSA
                     AFNLNVLEHVNRELGADFDLAKFSHRADYVRTKERMEMYLVSRVPQTVRIAGTEFAFD
                     AGDTIHTEYSYKYSLEHFRQLTASAGFTLAGQWTDPKNYFAVQYLVV"
     gene            complement(1715..3190)
                     /locus_tag="VHS97_06865"
     CDS             complement(1715..3190)
                     /locus_tag="VHS97_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3447957.1"
                     /translation="MASLAFLVALALVADEGPSTVRVGSKKFTESVILGDIAVGLVRE
                     AAVPAIHRRELGGTQVLFHALESDELDVYPEYTGTISGEILAGGKIRDEASLRTALAG
                     RGVGMSRPLGFNDTYAIGMREQVAARLGLMTLSDLRKHPELKFGFSNEFMDRADGWPG
                     IRDRYGLPQRDVRGLDHDLAYRAIASDEIQATDLYSTDAEIRQYNLRVLRDDLNFFPS
                     YECVWLYRADLQARSPTAFAALERLQGKVSSGEMAAMNARAKLDRVPEDRVAADFLAE
                     KFGIEAAVSTETWVGRLFRRLGEHLALVSISLAAAIVVSIPLGVIAARRPRLGPLILT
                     TTGLIQTIPSLALLVFMIPWLGIGAKPALAALFLYSLLPIVRNTATGLRDIPTSLRES
                     AEALGLPARARLWRIELPLAARSILAGIKTAAVINVGTATLGALIGAGGFGQPILTGI
                     RRDDVPMILYEGAIPAAVLALAVQAAFDLAERFVVAKGLRV"
     gene            complement(3190..>3810)
                     /locus_tag="VHS97_06870"
     CDS             complement(3190..>3810)
                     /locus_tag="VHS97_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3447958.1"
                     /translation="RVMIGLIQPDTGWVKIAGTELNPATILEVRHGLGYVIQDGGLFP
                     HMSARDNVILMAQYLRWDARKIEERLGELAELTRFPQDGLDRFPAQLSGGQRQRVSLM
                     RALMLDPKVILLDEPLGALDPMVRAELQADLRAIFRSLEKTVVMVTHDLAEASWFGHQ
                     IILLRDGRIVQRGPLRALIDSPAEPFVTQFVSAQRQVFELSVGGDS"
BASE COUNT          756 a         1149 c         1205 g          700 t
ORIGIN      
        1 aaaccgccga atgccgaagt gccacccacg aactggttgt tgtccaccgg cgaactaaaa
       61 tggtgcgggc ataacggcca agggtttgct ttcgacaacg aagggccgcg tcatcgcgtt
      121 tatttggaac cgttccagat cagctcacgg ccggtgacca gtggtgagta cgtggcgttc
      181 atcgaggatg gcggctatga tcggccggaa ctctggctct ccgacggttg gaacgctgtc
      241 aaaacccacc gctggaaagc gccgctttac tgggagcaag tcgccggcgc gtggtgggtg
      301 atgacgctgg gcggattccg gcgcctgacc gacgccgagc ccacttgcca tgtcagttat
      361 tatgaggcgg atgcgtttgc tcgttgggcg ggtgcgaggc tgccgacgga gtttgagtgg
      421 gagtcggccg ccatcgaaca ggaagtgaac ggcaatttcc tcgaaagcgg agacttacat
      481 cctcaacctg ctcttccagg cgcggaagtt gcttcacttt tcggcgacat ctgggtatgg
      541 acccagagcc cgtatgtccc ctaccccggt gctcgcccaa cggcgggcgc tctgggcgag
      601 tacaacgcca aattcatgtg taatcagctt gtgctccgag gtggttcgtg cgcgacgcca
      661 gcgtcgcaca tcagggcgac ttaccgaaat ttcttccccc ccgaggcgcg ctggcagttc
      721 acggggatcc gactggcgaa agacgcatga tttcaaccag ccttgcgata gtccgccaca
      781 atgagacatt gtcgcggcgc cgacagtttc tcaaggatgt cattcaagga ttgagccggc
      841 cgcaaaagga aatcgcctgc aaatatttct acgatgaggt tggatccgcc ctcttcgacg
      901 aaatatgcga actcgacgaa tattacttaa cgcggacaga aatggcgatc ctcgaagcgc
      961 acgcggccga aatggcagac gccatcggcg aggattgcga attgatcgag ctgggcagtg
     1021 gcaacgccca gaagacgcgg ctcttgctcg agcaacttcg gtccccgcgc gcttatctgc
     1081 cggtcgacat ctcacgcgaa ccgctcgagc tgtcggcctt gcgactggcc aagcagttcc
     1141 ctgagctgcg cgttctgccg gtccacggtg attttacagg accgctgttg ctgccggaaa
     1201 ccggcgaccc gcgggcacgc cgcgtcgtgt attttccggg ctcgaccatc ggcaacttca
     1261 gtcccagagc ggccaccagt ttactctgca caattgcctg gcttgtcggc gacggaggcg
     1321 ggctcttgat cgggtttgat ctcgacaagg atccacacat cgtctggccc gcatacaacg
     1381 acagtcgggg cgtcagcgcc gcatttaact tgaacgtgct cgaacacgtc aatcgcgagc
     1441 tgggcgccga cttcgacctg gcgaagtttt cccaccgggc ggattacgtg cgaacaaaag
     1501 agcgtatgga aatgtatctg gtcagtcgcg tgccacagac ggtcaggatc gccgggaccg
     1561 aatttgcgtt cgacgcgggc gacacgatcc ataccgaata ttcttacaaa tattcactcg
     1621 agcacttccg ccagctgacc gcatccgcgg gctttacact ggccgggcaa tggacagatc
     1681 cgaaaaacta tttcgcggtg cagtatttgg tggtttagac ccttagtcct ttggcaacca
     1741 cgaaccgctc ggccaggtcg aatgcggctt gaacggcgag agcgagtacg gcggcgggga
     1801 tcgctccttc atagaggatc atcggcacat cgtcgcggcg gatgccggtc aagatgggtt
     1861 ggccgaagcc gccggcaccg atcaaggcgc cgagcgtcgc ggtgccgacg ttgatgacgg
     1921 cggccgtttt gatccccgct aagatcgacc gcgcggccag cggtaactcg atccgccaga
     1981 gccgtgctcg cgccggtagt ccaagcgcct cggccgattc ccgcagtgag gtgggaatgt
     2041 cgcgcaggcc tgtggccgtg ttgcgaacga tcggcaacaa actgtagagg aagagcgccg
     2101 ccagggccgg tttggccccg atccccagcc agggaatcat gaacacgaga agtgccagcg
     2161 aggggatcgt ctggatcaac cctgtcgtcg tcaggatcaa aggcccgagc cgcgggcgcc
     2221 gcgcggcgat cacgccgagt gggatcgaga cgacgatcgc cgcggccagc gaaatggata
     2281 ccagagccag atgctcgccc agccgcctga agagtcgccc aacccacgtc tcggtactga
     2341 ccgcggcctc gatgccgaac ttctccgcga ggaagtcggc cgcaacacga tcttcgggca
     2401 cgcgatcgag cttggcccgg gcgttcatcg ccgccatctc gcccgacgaa acctttcctt
     2461 gcaaccgttc cagggcggca aaagccgtgg gggaccgcgc ttgcagatcg gcgcggtaca
     2521 gccagacgca ttcataagag gggaaaaagt tgaggtcatc gcgcaacacg cggaggttgt
     2581 actgccggat ctcggcgtcg gtcgaataga ggtcggtggc ctggatttcg tcgctggcga
     2641 tggcgcggta ggcaaggtcg tgatcgagac cgcggacatc gcgctggggc aagccataac
     2701 ggtcgcgtat gcccggccag ccgtccgccc gatccatgaa ttcgttgctg aatccaaact
     2761 tgagctcggg gtgtttacgc aggtcggaga gtgtcatcag tcccaatcgc gcggcgactt
     2821 gttcgcgcat gccgatggca tacgtgtcgt tgaatccgag cggccggctc attcccactc
     2881 cgcgcccggc caaggccgtg cgcagtgagg cttcgtcccg gatcttcccg cctgccaaga
     2941 tctcgccaga gatcgtgccg gtgtactcgg ggtaaacgtc gagctcatca gattcgagcg
     3001 cgtggaagag aacctgcgtt ccccccagct cgcgccggtg gattgccggt acggccgcct
     3061 ctcggaccaa accaacggcg atatcaccca ggatcacgga ttcggtgaac tttttcgagc
     3121 cgacccggac cgtcgacggg ccttcgtcag cgacaagcgc gagtgcaacg agaaaggcta
     3181 agctggccat caagagtctc cccccactga gagctcaaag acctggcgct gggcgctgac
     3241 gaactgggtg acgaagggct ccgccgggct atcgatgagc gcgcggagcg gcccgcgttg
     3301 cacgattcgg ccatcgcgca gcaggatgat ttggtggccg aaccagcttg cctcggcaag
     3361 gtcatgagtg accatgacga cggtcttctc gagcgatcgg aagatggcgc ggaggtcggc
     3421 ctggagctcg gcgcggacca tcgggtcgag agcgcccaag ggctcgtcga gcaagatcac
     3481 tttggggtcg agcatgagcg cacgcatcag gcttacgcgc tggcgctggc cgccggagag
     3541 ctgggcaggg aaccggtcga ggccgtcttg cgggaagcgc gtgagctcgg cgagctcgcc
     3601 cagccgctcc tcgatttttc gggcatccca ccgcaggtac tgcgccatca agatcacatt
     3661 gtcgcgggca ctcatgtggg gaaaaagacc gccgtcctgg atgacataac cgagtccatg
     3721 acgcacttcc aggatcgtcg cagggttgag ctcggtcccg gcaatcttga cccagccggt
     3781 atcgggctgg atcaggccga tcatcacccg
//
LOCUS       DATAQL010000399         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3246331, whole genome shotgun sequence.
ACCESSION   DATAQL010000399 DATAQL010000000
VERSION     DATAQL010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246331"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1354
                     /locus_tag="VHS97_06875"
     CDS             221..1354
                     /locus_tag="VHS97_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX3447959.1"
                     /translation="MLSVDGATALLVERGLIDPAWIIDGALTIKSAARRNRNLRVEGA
                     KGAGFLIKQPDNPGDGDFHLLQREAAFHRFCLEETAARPISTIVPRLVWSDNPETLLV
                     FELIAEATSLWSRLEAGVEERLTVDASRALGRTLGTFHRLLCLNDWKDDTRLARLSRG
                     LPWVLRVHQPVPSMLASVSRANLGLMRILQTEEGLGKHLDRLAERWRPSSVIHGDVRL
                     DNVLIRTQRAETEDGPVELWIVDWEAVGFGDPAWDVAGAFQDFLVYWIFSMPLSDELT
                     AEQMIAQARVPLGGLRTALRALWSGYKAGACLDGSEADDMLLRAVAFSAARLIQSAYE
                     ISSGSDSLVGPPVVLLQIAANLLAEPERGQIELYGIPLGCPIP"
     gene            1351..>3206
                     /locus_tag="VHS97_06880"
     CDS             1351..>3206
                     /locus_tag="VHS97_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector HopA1 family protein"
                     /protein_id="HEX3447960.1"
                     /translation="MNLIHDDLFTVLDAIEIESPVRYSLLGELRTLDAADPLEPGGLV
                     AVLAEEMYARLYVRPSFPQPRSRADVLAQRDFMAGLSAANSGRGTCEPGWKVRRIEED
                     GRALAEKNEVEFRVPALGWRGVRDGEIGPGAACKVAVPKERRYLIWGFYYAVGDAEEE
                     TYSGDAAVEPMVRFYWHLLSSVAAPFVAAATSALNAARIPFLLKVLSDPNDFHRADSG
                     VIFVRRHYLPRLSEMVAGIHSELRAGLRGEVPLFTRRLADGLGWAEDPANAMSFGQHR
                     CQLAARASWESFARGELDRDARALALAQAFRDEGLDPLRPYLRLDSPADDAVLPPFPP
                     PVSIQISVDRFGPDFAAEASRADGSTLSSIDAAARIGEALCRSAYWDRQGQSCNWMGR
                     SIAEIAQYGGPITPTVAACGPDLYAGSAGIGLFLAQLHALTGDLAFARTSLGAIDRSI
                     RQLASVPSTEAASPLSFHCGHLGVAYAARRVGLLTTHIEPVLQAVTILDRVINAVSSP
                     HELDVIGGNAGAIPILLLLGGESGLERCHDLAIALGEELCRTAVHQVSGWAWEPEAAS
                     GPGMGPVPQTGLSHGAAGMGMALFELYGATGRRDFLEIARGAFAFEDALFDP"
BASE COUNT          518 a          953 c         1075 g          660 t
ORIGIN      
        1 tgaagggcaa aatcgccgcg ggcctgacaa ccaagaaacc ggcaattcca tgtattgatg
       61 tgcatacgac tataccaata ccggttacca aactgccgtg tctgttttcg gcggtcgact
      121 tgtgcgtctc ggtgatcgag atttgcccgt agtgatggca tgggcgaaga aagagtcccc
      181 cggccgtgac ggtcaaacgt gacttgcgag gcatccggag atgctcagtg tcgacggtgc
      241 cacggcttta ctggtagaac gcgggctgat cgatcccgcg tggatcatcg acggagcgct
      301 gacgatcaag agcgccgcgc ggcgcaaccg caacctgaga gttgaaggcg ccaagggcgc
      361 cggcttcctg atcaaacagc cggacaatcc gggtgacggt gactttcacc tgctccagcg
      421 cgaagcggct ttccaccgtt tctgtctcga agagaccgcc gcgcgcccga taagcacgat
      481 cgtgccgcgg ctggtgtgga gtgacaaccc ggaaacgctt cttgtgttcg agttgatcgc
      541 ggaggcgaca tcgctctggt cacgattgga agcgggcgta gaagagcgac tgacggtcga
      601 tgcctcgcgt gcgctgggcc gtaccctggg tacgtttcac cgcctcctct gcctgaacga
      661 ctggaaagat gacacgcgat tggccaggct ctcacgaggc ctgccctggg tgctacgggt
      721 gcaccagcca gtgccctcga tgctcgcgag cgtcagccgg gccaatctcg ggttgatgcg
      781 gatcctgcag acggaggagg ggctgggaaa gcacctcgac cggctggccg agcgttggcg
      841 tccgagtagc gtcatccacg gcgatgtccg gctcgacaac gtactgatcc ggacgcagag
      901 ggcggagacc gaagacgggc cggtcgagct ctggatcgtc gattgggagg cggtggggtt
      961 tggcgatccg gcctgggacg ttgccggcgc gttccaagat ttccttgtct actggatctt
     1021 ctcgatgccc ttgtccgatg agttgaccgc cgagcaaatg attgcccagg cgcgggtgcc
     1081 attggggggg ttgcgaaccg ccctacgggc gctgtggtca ggctacaagg cgggggcgtg
     1141 cctggatggg tccgaggcgg atgacatgct gctgagagct gtggcgtttt cggccgcccg
     1201 gctaattcaa tcggcctacg agatctcgtc agggtcggat agtcttgttg gtccgccggt
     1261 ggtgttgctg caaattgctg ccaatctact ggcggaacct gagcgcggtc agatcgagct
     1321 ttacggtatt cccctcggct gccccatacc atgaatctca tccacgatga cttattcaca
     1381 gtgctcgatg cgatcgagat cgaatcgccc gtccgctatt ctctccttgg tgagctccgc
     1441 acgctcgacg cggccgaccc gctcgaaccg gggggactgg tggcggtgct ggctgaggaa
     1501 atgtacgcgc ggctctatgt tcgtccttca tttccgcaac ctcggtctcg cgccgatgtg
     1561 cttgcacagc gcgacttcat ggcgggcctg tctgcggcca acagcggcag ggggacgtgc
     1621 gagccaggtt ggaaggttcg tcgcattgaa gaggacgggc gcgctctggc cgagaaaaac
     1681 gaggttgagt tccgggtgcc cgccttgggt tggcgaggtg tgagggatgg cgagattggg
     1741 ccgggcgcgg cctgcaaagt cgctgttccc aaggagcgcc gttacttgat ctggggattc
     1801 tactatgccg tgggcgatgc cgaggaagag acgtactccg gcgatgccgc ggtcgagccg
     1861 atggttcgtt tttactggca tctgctctcc agtgtcgccg cgccattcgt ggccgccgcc
     1921 acgtcggctt tgaatgcggc tcgaatcccg tttctgctca aggtgctcag cgaccccaac
     1981 gactttcacc gcgctgattc cggagtaatt ttcgtacgtc gacattatct accgcgattg
     2041 agcgagatgg tcgccgggat ccacagcgag ctcagggccg ggctgcgcgg cgaagtacca
     2101 ctgtttacca ggcggctggc ggacggcttg ggctgggccg aggacccagc caacgcgatg
     2161 agctttggcc agcatcgctg ccagctcgcc gcgcgggcct cgtgggagtc gtttgcgcgg
     2221 ggcgagctcg accgtgacgc gcgcgcactg gctttagcgc aggcgtttcg tgacgagggt
     2281 ctcgacccgt tacgccctta tctccggttg gactcgccgg ccgacgacgc ggtcttgccg
     2341 ccgtttcccc cgcctgtttc cattcaaatc tccgtcgacc gattcgggcc ggactttgca
     2401 gcagaggcca gtcgggcgga cggctcgacg ttgtcgtcca tcgacgccgc cgctcgtatt
     2461 ggggaggcgc tctgccggtc ggcctactgg gaccggcagg gccagagttg caactggatg
     2521 ggccgctcga tcgccgagat cgcgcagtat ggcgggccca tcacgccgac agtagcggct
     2581 tgcggccccg acctctacgc tggatcagcc ggaatcgggc tctttctggc gcagctccat
     2641 gcgctcacag gcgacttggc gtttgcgcga acttccctgg gtgccatcga ccgttcgatc
     2701 cgtcagctcg ccagcgttcc ttccacggag gctgcctcac ccctctcttt ccattgcggc
     2761 cacctgggcg tggcgtacgc ggcccgcagg gttggtttgt tgaccactca tatcgagcct
     2821 gtcttgcaag ccgtcacgat ccttgatcgg gtgatcaatg ccgtttcgtc gcctcacgag
     2881 ctggatgtga tcggcggcaa tgccggcgcg atcccgattc tattgttgct ggggggcgaa
     2941 tcgggtctgg agcgctgcca tgatctggcg attgctctgg gtgaagagct gtgccgcacc
     3001 gcggttcacc aggtttcggg gtgggcttgg gagcctgagg ccgcatcggg acccgggatg
     3061 ggtcccgtgc cgcaaaccgg cctgtctcac ggtgcggccg gcatgggcat ggcgcttttc
     3121 gagctgtacg gagcgaccgg gcgacgcgac tttctggaga ttgcccgcgg ggcgtttgca
     3181 ttcgaagacg cgctcttcga tccaga
//
LOCUS       DATAQL010000400         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3856627, whole genome shotgun sequence.
ACCESSION   DATAQL010000400 DATAQL010000000
VERSION     DATAQL010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3856627"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VHS97_06885"
     CDS             complement(<1..260)
                     /locus_tag="VHS97_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447961.1"
                     /translation="MLLIALASTLISLSPDYSAAGQEAAATAPTSPKPARPRVKSLEV
                     VILSTMLTDRAGIGEWGFSALVVADGRRLLFDTGARPETVLR"
     gene            complement(377..751)
                     /locus_tag="VHS97_06890"
     CDS             complement(377..751)
                     /locus_tag="VHS97_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447962.1"
                     /translation="MTDKPVAALLADLKRTGLLEDTLVVWGGEFGRTPVRQGGGRGRD
                     HNATGFTMWMAGGGIKGGRTVGATDEAGLLAIEDRAHINDIHATILHLMGLDHKQLTF
                     LHNGRDERLTDVGGRIVKNLLA"
     gene            complement(748..>1801)
                     /locus_tag="VHS97_06895"
     CDS             complement(748..>1801)
                     /locus_tag="VHS97_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447963.1"
                     /translation="PGPQPFTPRSRREFLKVAGCGFGLLGLADLMARDALSATPSGPL
                     TPRPGHVPAKAKRCIFLFMTGGPSQMDMFDPKPLLNRLDSQPLPPSFGKIHSQFLEAD
                     PLCLGSHRKWGKYGQSGMDMSDLVPHLHAHADDIALVRSCYADSVIHAPAMYQMMTGR
                     INMGFPSLGSWVTYGLGSESDNLPAYVVMTQPEGTPEGGAPCWGAGFLPAHYQGTLFR
                     SGPNPIVNLEPPKGVSFSRQRGMLDLLRSMNEQDLDPGDTELSARIASYELAFRMQSS
                     APEAVDLSRESESTKALYGLDDPRTAEFGTRCLMARRLVERGVRFVQLYSGGGPVAVS
                     GTPMTTSTPTTKRCAG"
BASE COUNT          353 a          564 c          570 g          314 t
ORIGIN      
        1 ttgcgcaaga cggtctcggg ccgagcaccg gtgtcgaaca ggagccgacg gccatccgcc
       61 accaccaacg ctgaaaaacc ccattcgccg attccggcgc ggtcggtcag catcgtcgag
      121 agaatcacaa cttcgagcga cttgacgcgg ggacgcgcgg gttttggcga tgtcggcgcg
      181 gtggccgccg cctcttggcc tgctgcactg tagtcaggtg aaagagaaat gagcgttgat
      241 gcgagggcaa tcaacagcat attgacgcgc agtacgaccg cccaagatct gcggcagctg
      301 gaccaatgac ggtcgcgtct cacgaaaaaa tacggcgcca tggtctactc gacgaaaaaa
      361 agagccagca gccgcatcaa gccaacaaat tcttgacgat acgaccgccg acatcggtca
      421 accgctcgtc gcggccgttg tgcaggaagg taagctgttt gtgatcaaga cccatgaggt
      481 ggagaatcgt cgcgtggatg tcgttgatat gggcgcggtc ctcgatggcc aggagcccag
      541 cctcgtcggt ggcgccgaca gttcttcccc ccttgatgcc gccgccggcc atccacatcg
      601 tgaaaccggt ggcgttgtga tcgcggccgc ggccgccacc ttggcgcacc ggcgtacggc
      661 caaactcgcc cccccagacg accagggtgt cttcgagcaa acccgtgcgc ttgagatcgg
      721 ccaggagcgc ggccacgggt ttgtcggtca tcccgcacat cttttcgtgg ttggcgtcga
      781 tgtcgtcatg ggcgtcccac taacggccac cgggccaccg ccggaataaa gctggacgaa
      841 ccgcaccccg cgctcgacca acctgcgtgc catcagacag cgcgtgccga actcagccgt
      901 ccggggatcg tcgagcccgt agagggcctt ggttgactcg gattcccgtg acaggtcgac
      961 tgcctcaggt gccgagctct gcatccgaaa tgccagctcg tagctagcga tccgcgccga
     1021 cagttcggtg tcgccggggt cgaggtcttg ctcgttcatc gaccgcagca ggtcaagcat
     1081 cccgcgctgg cgagagaacg aaaccccctt gggcggctca agattgacga tcggatttgg
     1141 gccgctgcgg aacaaggtcc cctggtaatg ggccggcaaa aacccggccc cccagcaggg
     1201 agccccaccc tctggcgttc cttccggctg ggtcatgacc acataggccg gcaggttatc
     1261 gctctcggaa ccgagcccgt aggttaccca gcttcccaag ctgggaaagc ccatgttgat
     1321 ccgcccggtc atcatttggt acatggccgg cgcatggatc acgctatccg cgtagcacga
     1381 tcggacgagc gcgatatcgt cggcgtgggc gtgcaggtgc ggcacgagat ccgacatatc
     1441 catgcccgat tgtccgtatt ttccccactt gcggtgacta cccaggcaga gcggatctgc
     1501 ctcaaggaac tggctgtgga ttttcccgaa gctgggaggc agcggctgcg agtcgagccg
     1561 gttcaagagt ggcttgggat cgaacatgtc catctggctc gggccgccgg tcatgaagag
     1621 aaaaatgcac cgcttggcct tggcgggaac atgaccgggc ctcggcgtca gtgggcccga
     1681 aggcgtggcg gagagcgcat cgcgcgccat caagtcggcc agcccgagca ggccaaatcc
     1741 gcagccggcg accttgagaa attcccggcg acttcgtgga gtgaacggtt gcgggccagg
     1801 a
//
LOCUS       DATAQL010000401         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4467077, whole genome shotgun sequence.
ACCESSION   DATAQL010000401 DATAQL010000000
VERSION     DATAQL010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4467077"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..2944)
                     /locus_tag="VHS97_06900"
     CDS             complement(8..2944)
                     /locus_tag="VHS97_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX3447964.1"
                     /translation="MRRRLWIFLVGLLAACGGFWAFDVWRSRADWQQAQRDMARGKPA
                     SALPRLARLAGRWQGDGAVQYELGLCELALGHVDFAEAAWARVPSASPLAAQAAMMRA
                     RQKLKRHRLSDAEPLLQLALDDRGEFGKEARESLVHLCKLQGRYDEARRLVREGWGRY
                     DRVGTIQELVRLDTSNPIPVEKIQPILNTAAEAAPDDDRIWLGWANLATRIGRFEVAR
                     KWLDACVQRRPADPAVWRVRLDWARAAENESEVRRALAHLPSDGVPRTELLSLGAWFA
                     RRAGDAEAERRALEELVECNPGALAAMESLAELQLQSGQTERAKQLRVRKGELEQTLD
                     WYMVNIFPTDRLEHATELARAAEALGRRFEARCWWELAAEQSRHTALATAELARLDRE
                     ETSRPSPRHLTPAGLLAELNAEPKRVSHSPRAESCGATPRFVDGTDSAGLHFTFDSGL
                     ETLHQMPETMSGGVALLDYDGDGWLDVYAIQGGKFPPSADAPNTGDRLFRNKGNGKFE
                     DVTERSRIAGLRRGYGHGVTVGDIDNDGHPDLFLTRWQSYVLLRNQGDGTFEDITVKA
                     GLGGDRDWPTSAAFADLDGDGDLDLYVCHYLKWDSEHPRTCLDKDRKVFTFCGPPEFP
                     PMPDHLFRNDGGHFVDISTEAGIVDRTGEGLGVVANDLDGDGRVDLVVANDQSAKFLF
                     LNRGGLHFEEVGHLAGIASTGSGLYQASMGIATGDLNGDGRPDVAVTNYYNEYTALYQ
                     NLGDGVFSDHSADYGLAVASRYRLGFGIAFLDFNNDGRLDLATANGHVDDHRTDVPQR
                     MRAQLFAGAADGKKLVDVTDRAGPVFQVPLLGRGLAVGDLDNDGRVDLLILPQNQPLV
                     YYHNQSDGGRSLTLLLEGTSSNRDAVGARVVVEFDGRRQYAWRTGGGSYQSASESRIH
                     LGLGAVDRVDLVEVTWPSGRVDRYTGLQAGRGYRLREGDPSSKTLPGFRHGNEGVANR
                     RLLGND"
     gene            complement(3086..>3218)
                     /locus_tag="VHS97_06905"
     CDS             complement(3086..>3218)
                     /locus_tag="VHS97_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEX3447965.1"
                     /translation="HPGGANVLFGDGSVKFIKSSISYPTWWALGTKDGGEVVSADAY"
BASE COUNT          617 a         1020 c          970 g          611 t
ORIGIN      
        1 ggattgttta atcattaccc agcaagcgtc ggttcgcaac cccttcgttt ccatgacgaa
       61 agcctggcag agtcttggag gatgggtctc cttcacgaag ccggtagcct cgaccggcct
      121 gcagtccagt gtatcggtcg acccggccgg atggccacgt cacttcaacc aagtcaacgc
      181 gatcaaccgc tccgagtccg aggtggattc gagactctga ggcggattgg tagcttcccc
      241 cgcccgttcg ccatgcatat tggcgacgcc catcgaattc aacaacgacc cgagccccga
      301 cagcatctcg attcgaggac gtgccttcga gaagtagggt gagtgaacgc ccgccgtcgg
      361 attgattgtg gtagtaaacc aggggttggt tctgtggaag tatcaacagg tcgacccgtc
      421 cgtcgttgtc cagatctccc acggccaaac cgcgtccgag aagtggaacc tgaaagacag
      481 gtccggcacg atcagtcacg tcgacgagtt ttttcccgtc cgctgcgccc gcgaaaagct
      541 gcgcccgcat ccgttgcgga acgtcggttc ggtgatcgtc gacatggcca ttcgccgtcg
      601 ccagatcgag ccggccatcg ttgttgaaat cgagaaatgc gatcccgaag ccgagccgat
      661 agcggctagc caccgccaag ccgtaatccg cggaatggtc gctgaacaca ccgtcgccta
      721 ggttttggta cagggccgtg tattcgttgt agtagttggt gacggccacg tcgggccgcc
      781 cgtcgccgtt aaggtcgcca gtcgcgatac ccatgctcgc ctggtataac ccggacccgg
      841 tgctcgcgat tccggcaagg tggccgacct cctcaaagtg aaggcctccg cgattcaaaa
      901 aaaggaattt agccgactga tcgttagcaa ccacgaggtc gacacgccca tcgccatcaa
      961 ggtcgttggc gacaacccca agcccctcgc cggtccggtc gacaattccc gcctcggtcg
     1021 aaatatcgac gaaatgcccg ccgtcgttgc ggaagaggtg atcgggcatt ggcggaaatt
     1081 caggcggacc acagaaggtg aatactttgc gatctttgtc caagcacgtg cgcgggtgct
     1141 cgctgtccca cttgaggtag tggcaaacat agaggtcgag gtcgccgtca ccatcgaggt
     1201 ctgcaaaggc tgccgacgtc ggccagtcac ggtcacctcc gagaccggcc ttgacggtaa
     1261 tgtcttcgaa ggtaccgtcc ccctggttgc ggaggaggac ataggattgc cagcgggtca
     1321 ggaacagatc ggggtggcca tcgttatcga tgtcgccgac agtcacaccg tggccataac
     1381 cccgacgcaa ccccgcgatt cgcgatcgct cggttacgtc ctcgaatttc ccgttgccct
     1441 tgttccgaaa aaggcggtca ccggtattag gcgcatcggc gctcgggggg aacttgcctc
     1501 cctgaatggc gtagacgtcg agccagccgt cgccatcgta gtcaagcagg gcgaccccgc
     1561 cgctcatcgt ttcgggcatc tggtgaaggg tttccagtcc gctgtcgaac gtgaagtgca
     1621 gtccggccga atcggtacca tcgacgaacc ggggtgtcgc gccgcaggac tctgcccggg
     1681 gtgagtggct tactcgtttc ggttccgcgt tcaactcagc cagtaggcca gcgggcgtca
     1741 ggtggcgggg cgacgggcgg gaagtctctt cgcggtcgag ccgagcgagt tccgccgttg
     1801 ctaaagcggt gtggcgggat tgttcggcgg cgagttccca ccaacaacgg gcctcgaacc
     1861 ggcggccgag tgcttcggct gcgcgagcca gttcagttgc gtgctcgagt cggtctgtag
     1921 ggaaaatgtt gaccatatac cagtctagtg tctgctccag ctcgcctttc cgcacacgga
     1981 gttgtttggc cctctcagtt tgaccggact ggagctgcaa ctccgcgagg ctctccatcg
     2041 cggcaagggc ccccgggttg cattcgacca gttcctccaa tgcgcggcgc tccgcttcgg
     2101 cgtcacctgc acggcgagca aaccacgcgc ccagcgagag cagttcggtc cggggaactc
     2161 cgtcagacgg tagatgcgcc agggcgcgcc ggacctcgga ctcgttttcg gcggcgcgag
     2221 cccagtcgag cctgactcgc cagacggccg gatcggcagg ccggcgctgg acacaggcat
     2281 caagccactt ccgggcgact tcaaaccggc cgatgcgagt cgccaaattg gcccagccca
     2341 gccagatgcg gtcgtcgtca ggagcggcct ctgccgccgt gttgagaatg ggctgaatct
     2401 tctcgacggg aatgggattt gaggtatcga gtcggacaag ttcctggatg gttccgacgc
     2461 ggtcgtagcg tccccagccc tcgcgaacga gacggcgagc ctcgtcgtaa cgcccctgaa
     2521 gcttgcagag gtgaacgagc gactccctgg cttctttccc gaattcgccg cgatcgtcaa
     2581 gggccaattg caagagcggc tcggcatccg agagccggtg gcgcttgagc ttctggcgtg
     2641 ccctcatcat cgcagcctgc gccgcaagtg gcgaggccga aggaactcgc gcccatgctg
     2701 cctcggcaaa gtcgacatgc cccaaggcca gctcgcagag cccaagctcg tactgaaccg
     2761 ctccatcacc ttgccaccga cccgccagtc tggccagcct gggcaaggct gacgccggct
     2821 ttcccctggc catgtcgcgc tgcgcctgct gccaatcggc ccgtgatcgc cagacgtcga
     2881 atgcccagaa tcccccgcaa gccgccaaaa gtccgaccaa gaagatccac agtcgccgtc
     2941 gcacccgacc ctcccaccag cgcaagcacg aaaccacatc ggaggggatc atttccggcg
     3001 tcattggggt ttatttgatc agatacgatc ataattgcgt ggaccggccg tcaagccggt
     3061 ccaccattga tcgacttcgt gattctcaat acgcgtcagc gctgaccact tcgcccccat
     3121 ccttggtccc tagcgcccac caggtcgggt agctgatgga actcttgatg aatttaacgg
     3181 acccgtcacc gaagagcaca ttggctccgc cggggtgg
//
LOCUS       DATAQL010000402         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1871829, whole genome shotgun sequence.
ACCESSION   DATAQL010000402 DATAQL010000000
VERSION     DATAQL010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871829"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VHS97_06910"
     CDS             <1..499
                     /locus_tag="VHS97_06910"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX3447966.1"
                     /translation="IQRRKTLWFEPYHQALSSELARLRTIHDRVVLYDCHSIRSIIPR
                     LFDGILPHFNLGTNGGASCAPELTAAVEEACEATSLSRVTNGRFTGGWITRQYGRPQA
                     GVHAIQLELACRVYLAEPIGRVDETSWPVAFDNAYAAPMAAVLSKVLQLCVEFAIGTT
                     RGATP"
     gene            496..2163
                     /gene="hutU"
                     /locus_tag="VHS97_06915"
     CDS             496..2163
                     /gene="hutU"
                     /locus_tag="VHS97_06915"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX3447967.1"
                     /translation="MTRIDKTRTVRAPRGSTLSAKSWLTEAALRMLMNNLDAEVAERP
                     EELVVYGGIGRAARDWESFDRIVSTLRRLEDDQTLLVQSGKPVGVFRTHRDAPRVLIA
                     NSNLVPAWASWDHFHELDRKGLMMYGQMTAGSWIYIGTQGIVQGTYETFAELARQHYR
                     GSFAGRWILTAGLGGMGGAQPLAAVMAGASCLAVECQPSRIETRLRTGYLDRQSTSLD
                     ESLEIIDRSCRDGAPVSVGLLGNAAEIYPELVRRGVRPDAVTDQTSAHDPVNGYLPRG
                     WTLEQWARARVHDPKGVERAAKKSMAEQVSSMLDFHKAGVPTFDYGNNIRQMAKDEGV
                     ADAFAFPGFVPAYIRPLFCRGIGPFRWVALSGDPEDIYRTDAKVKALLPDNVQLHGWL
                     DMAREKIKFQGLPARICWVGLGDRDRLGLAFNQMVADGELKAPIVIGRDHLDSGSVAS
                     PNRETEAMKDGSDAVSDWPILNALLNCASGATWVSFHHGGGVGMGYSQHAGLVIVADG
                     SPEAARRLERVLWNDPATGVMRHADAGYSAAVECARAKGLDLPSIPS"
     gene            complement(2259..2981)
                     /locus_tag="VHS97_06920"
     CDS             complement(2259..2981)
                     /locus_tag="VHS97_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447968.1"
                     /translation="MSFESYERLPRHPSYKFEYWDGQLRISPRWASHSMFLELRPPGT
                     RDLVDDPRVASIRPLASDDWEVLPEVLATAFEDTPPLGMLGYVRRVWAARDWLWSTRD
                     GNEGPLVEPACMVAVDREDNSKVLGALVVTLMIGRTRSWYARRRLAVPPPPPQLAEDQ
                     GQPQVSWVFVRRGASDQGVGSALLASATSSLWSAGYRELASATDRGNVLSMAWHWRNG
                     FRLLPHREPIRPIKTGDRQPDP"
     gene            complement(3039..>3338)
                     /locus_tag="VHS97_06925"
     CDS             complement(3039..>3338)
                     /locus_tag="VHS97_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent
                     methylenetetrahydromethanopterin
                     dehydrogenase/methylenetetrahydrofolate dehydrogenase"
                     /protein_id="HEX3447969.1"
                     /translation="CAVVVCAGASGVTLLPASAWRKLTALKVLIDLNAVPPLGIEGIE
                     ATDKNAERDHVRAWGALGVGGTKMKIHKKAVQELFTSNDKILDAEEVLELGRSLS"
BASE COUNT          627 a         1051 c         1036 g          624 t
ORIGIN      
        1 aatccagaga cgtaagacac tctggttcga gccctatcac caagcgctct cgagcgagct
       61 cgcacgattg cgcacgatcc atgaccgcgt cgttctctac gactgccatt cgatccgttc
      121 gataatcccg cgtttgttcg atggcatctt gccccacttc aacctgggca ccaacggcgg
      181 agcgagttgt gcgcccgaac tgacggcggc ggtagaggag gcgtgcgaag cgacgtcatt
      241 gagccgtgtc accaacggtc gatttacggg aggatggatc acgcgccaat acggcagacc
      301 gcaggcgggc gtgcacgcga tccagcttga actcgcatgc cgtgtttatc tcgccgaacc
      361 gatcggccgc gtcgacgaga catcctggcc cgtcgccttt gacaacgctt acgcagcgcc
      421 catggcggct gtgctgagca aggtcttaca gttgtgcgtt gagtttgcga tcggaacaac
      481 ccgaggagca acgccgtgac ccgcatcgac aaaacacgca ccgtccgcgc cccccgcggt
      541 tcgacattga gtgcgaagag ctggctgacg gaagcggcgt tgcggatgct gatgaataac
      601 ctcgacgccg aggttgccga acggcccgag gaacttgtcg tttacggcgg catcggacga
      661 gcggcgcgcg actgggagag cttcgatcgc atcgtgtcta cgctgcgcag gctcgaggac
      721 gaccagacgc tactcgtgca gtccgggaag ccggtcggcg tgtttcgcac gcatcgcgac
      781 gcgccgcggg tcctgatcgc gaattccaat ctcgttccgg cgtgggcaag ctgggaccat
      841 tttcacgagc tcgaccgcaa agggctcatg atgtatggcc agatgaccgc gggaagctgg
      901 atctacattg gaacgcaggg aatcgtgcaa gggacctacg aaacatttgc cgagctcgcg
      961 cggcagcatt atcgcggcag tttcgccggt cgctggatcc tgaccgccgg cttgggcggc
     1021 atggggggcg cccagccttt ggcggctgtg atggcgggtg cctcttgcct cgccgtggaa
     1081 tgccagccgt cgcgcatcga gacgcgcttg aggaccggtt acctcgaccg gcaatccaca
     1141 agtctggatg aatccctcga gatcatcgat cgatcgtgcc gcgacggagc cccggtttcg
     1201 gttggtctcc tgggcaacgc cgccgagatc tatcccgagt tggtgcgccg aggcgtgcgg
     1261 cccgatgcgg tcactgacca gacctcagcc catgacccgg tcaacggcta tctcccgcgg
     1321 ggctggaccc tcgagcaatg ggcccgggct cgcgtgcacg atcccaaagg ggttgaacga
     1381 gccgccaaga aatcgatggc cgaacaggtg agctcgatgc tggattttca caaggcagga
     1441 gtgccgacct ttgattatgg caacaacatc cgccagatgg ccaaggacga gggagtcgcc
     1501 gacgcatttg ccttccccgg cttcgtcccg gcctatattc gcccgttgtt ctgccgagga
     1561 atcgggccgt tccggtgggt tgcactctcg ggtgatccgg aggatatcta ccgaaccgac
     1621 gcaaaagtga aagcgctctt acccgataac gttcagcttc acggttggct cgacatggcc
     1681 cgcgagaaga tcaaatttca gggcctgcca gcccgtatct gctgggtagg actgggagac
     1741 cgcgaccggt tggggctggc gttcaatcaa atggtggccg acggcgaact gaaggcgccg
     1801 atcgtcattg gtcgcgacca tctcgactcg ggctcggtcg cctcccctaa ccgcgaaacc
     1861 gaggcgatga aagacggttc cgatgctgtg tcggactggc cgatcttgaa tgcgctcttg
     1921 aactgtgcct cgggcgccac ctgggtgtcg tttcaccacg gcggcggcgt cggcatgggc
     1981 tactcgcagc acgccggctt ggtgatcgtg gccgacggta gcccggaggc cgcgcgccgg
     2041 ctcgaacgcg tgctctggaa tgacccggct acaggggtca tgagacatgc cgacgcgggc
     2101 tattccgccg cggtcgagtg cgctcgtgca aagggcctgg acttaccttc gattccaagt
     2161 tgatgaaagt cacgcggtct ccgctgcgga tcactcggcg atcaagtgtg accaggcaac
     2221 gcccttctga tcgacaaacc tcggtccggt ctgatcgctc aagggtccgg ctgacggtcc
     2281 ccagttttga ttgggcggat gggttctcga tgtggtagca gccggaaccc gttgcgccaa
     2341 tgccacgcca tgctcaatac attgccgcga tcggtcgccg acgccaattc gcggtaaccg
     2401 gcactccaca gcgaacttgt cgccgatgcc agtaacgccg atcccactcc ttggtcggat
     2461 gcccctcgcc ggacgaaaac ccaactgacc tggggctgac cttgatcctc cgccaactgc
     2521 ggcgggggcg ggggtactgc aaggcgtcgc cgcgcatacc aggaacgcgt ccggccgatc
     2581 atcagggtta ctacgagcgc ccccagcacc ttggagttgt cctcccggtc gacggcgacc
     2641 atgcaagccg gctcgaccag cgggccctcg ttgccgtcgc gggtagacca cagccaatcg
     2701 cgggctgccc acactcgccg cacgtaacca agcatcccca gagggggagt atcctcgaac
     2761 gccgtcgcca ggacctcggg caacacctcc caatcgtcgc tcgctagagg gcgaattgat
     2821 gcgacgcgcg gatcgtcgac caggtcgcgc gtccccggag gccgcagctc caggaacata
     2881 ctatggcttg cccatcgcgg gctaatccgc aattggccgt cccagtactc gaacttgtac
     2941 gaagggtgtc gcgggagtcg ctcatatgac tcgaacgaca tcggtacacg gaccggcctg
     3001 aaccagcgat cgatatcttt cctgttcata aggtgcaact aactgaggga tcgccccagc
     3061 tcaagcactt cctcggcgtc gagaatcttg tcgttggaag taaacagctc ttgaactgct
     3121 tttttgtgga tcttcatctt ggtgccgccg acgcccagcg cgccccaggc gcggacgtgg
     3181 tcgcgctcgg catttttgtc ggtggcctcg attccttcga tgcccagggg agggacggca
     3241 ttcaggtcga tcaagacctt gagcgccgtc aatttccgcc aggctgatgc cggcaggagt
     3301 gtcactcccg acgcccccgc gcagacaacc acagcaca
//
LOCUS       DATAQL010000403        10182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4771845, whole genome shotgun sequence.
ACCESSION   DATAQL010000403 DATAQL010000000
VERSION     DATAQL010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4771845"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..562
                     /locus_tag="VHS97_06930"
     CDS             5..562
                     /locus_tag="VHS97_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447970.1"
                     /translation="MHGANGYLPDQFLQDSTNHRTDEYGGPIENRARFLLDVTDAAIS
                     VWGPDRVGVHLAPRCDVHSMGDSDRAATFGYVARELGRRRIAFICAREALGDDRLGPQ
                     LKVAFGGNYIANERFTKKTGDRVLAAGEADAVAFGVQFIANPDLPRRFALDAPLNEPN
                     PETYYASGPEGYIDYPSLEAQGAAV"
     gene            complement(587..1636)
                     /locus_tag="VHS97_06935"
     CDS             complement(587..1636)
                     /locus_tag="VHS97_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3447971.1"
                     /translation="MLTRRDFLGAIPPAVLLTNARAGEAPEFGRIDTHIHIHRDAREL
                     LSALKAANWRGLDIVVCPASGDEPFDLEEKLGATLKVARDSGGTLAWASTFDARGFEE
                     PNFAERTIARLRRSFEDGAIGVKIWKNIGMSIKAKSGAYLLPDDPALLPIYEAIERAG
                     RTLVAHLAEPDGAWLPLDSKNPELSYYSNNPQWHMFGKAGAPVKHDILTARDRVLARY
                     PKLRVVGCHLGSDEDDLKRLAKRLDAYPNFAVDTAARVRYFARGDRDQVRQFLTHYQD
                     RILYATDFSLRTGDDATAARALKVTHDRDWAFFSGNEKMDYAGHATQGLALPDQVLSK
                     IFRENALRWLPGFSE"
     gene            1792..4797
                     /locus_tag="VHS97_06940"
     CDS             1792..4797
                     /locus_tag="VHS97_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3447972.1"
                     /translation="MPAAGDDFPEPPNTERSSTRPLSPADAAAGWRVPEGFRVNVFAA
                     EPDVRNPVAMAWDTRGRLWIAENYTYSDTTQRFDLRLRDRVLILEDNDADGRMDKRKV
                     FTDGVQRLGSVELGFGGVWLLCPPQLLFVPDHNGDDVPDGPAEVMLDGFAVATDNHHT
                     FANGLRWGPDGWLYGRCGASSPGQVGVPGTRDAQRIPIRGGLWRYHPIRKRFEVLSHG
                     TTNPWGHDWNAVGEAFFINTVNGHLWHMIPGAHFARPHTIEPNPRVYAVIDQHADHYH
                     WDNSRHYKNTLIPGGIDDHRGGGHAHSGVMIYLGGQWPESFQDKLVTLNFHGRRANVE
                     RLERLGSGYVGRHEPDMFFAADPWFRGVDLSYGPDGSMSVLDWSDTGECHEHDGVHRS
                     SGRIYRFTYGKPARVDLRDVSKLEERDLVALHLHANEWFARQARRVLAERSTRGEPLS
                     TAKASLREIFEQTPDPSRKIRALCSLFVIGGTDAPFLRGLLRHEHESVRAWAVRLLTD
                     MLPIDTIYSRRGGPDVELPADLRSRFADLAMTDPSGLVRLVLASTLQRLPVGQRESLA
                     HALLSRSEDDADHNIPSLIWTGLIPVAEADPEALVRLAVDCRLAHVQRLIARRLGEEI
                     ETHPGPVNALLEAASQRPEGLVFQTQLVAGLNDALTGWRKATRPAAWDPFQQKLSTAA
                     DPALRDRIRDLNVLFGDGRALEEVKRLALDGTASLDVRMSALKSLIEARPPDLRAVCE
                     RLVRVSFLNAVAMRGLALFDDPAIGVTLAKSYRSFHPSERSAVVETLASRPAFALALL
                     DQIAAGGIPRQDLSAFHARQIRGLGASAITERLAQVWGVERDSADDRREQIATLKRKL
                     GSSALAQPDRRRGRAVFDRVCGTCHKLYGYGGEIGPDLTGAGRDNLDYLLENLVDPSA
                     SVSADFRMVVVAMNDGRALNGLVRARTDRTLTLQTQTAALVLDRQEIETVQPSPLSLM
                     PDGLLAGLSETETRDLLAYLMHQTQVPLP"
     gene            complement(4794..5786)
                     /locus_tag="VHS97_06945"
     CDS             complement(4794..5786)
                     /locus_tag="VHS97_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3447973.1"
                     /translation="MAPPVGLRTNKVRRQGQEAMEVRSTPSSPRVEGQEIARFLIDQG
                     ELRSILSNLSHELCRPLVSLRAGFDLLLGDSPSNITPDQQGHLLTMVSLCDDLLRLTR
                     SYLDYAGIVQGSRPLCLGSFTIGALIDELDRQFAPVAQSRQLVWESRAQAPEMVVVTD
                     ASRCQQIFGNLVSNAIKYTPVGGQVRVAGKVDTDSWTVVVADSGPGIPAEALDRVFEP
                     FFRLDRDEHSIIEGSGLGLAICRELVAQMQGDISVDSVAGKGTSICVRFPMNAAGPTQ
                     SSSLAENAAVAGRDRTTSTSADGSSVLAAGVDRASKKFGHATGRPVVHPGRTEA"
     gene            complement(5812..6072)
                     /locus_tag="VHS97_06950"
     CDS             complement(5812..6072)
                     /locus_tag="VHS97_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447974.1"
                     /translation="MPMYNSRMELRRFGFGKNNLELAEFIEGHINRVANGRIRDLHVV
                     YSDDKIILQGHSRTYHAKQLAHQAVLDLTDGHPLLANQIVVS"
     gene            complement(6360..7016)
                     /locus_tag="VHS97_06955"
     CDS             complement(6360..7016)
                     /locus_tag="VHS97_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3447975.1"
                     /translation="MTRPYRILIADDEKTVAVGLQNQLESLGYDVVAAVSDGHRAIEM
                     CRRSIPDVVFMDIEMPGLDGLSAARQIAADPGTPVIILTAHGHPNLIDQAVEDGVISY
                     LLKPATNPSLHAAIQVAVARARELKLLQDDVDQLKSTLRERKLIERAKGILMTRKQLS
                     ESEAFRLLQRQSQDRRIPMAKLAESIIQTDELFEAQDQPPPPSASRPLRRHFELPQGE
                     "
     gene            7382..7852
                     /locus_tag="VHS97_06960"
     CDS             7382..7852
                     /locus_tag="VHS97_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX3447976.1"
                     /translation="MVGDEWESPRRTVTETDVVLFAGLSGDFNPLHVDHSSFRSSPFG
                     RPVAHGLLGLAIASGLMSQAPRVDTLAFLAILEWKFHKPILFGDTIHARYTIESLQSQ
                     AKGRRGVVTWHRRIFNQDDNLVQEGRTQTLVRARTRSRSSKRTESDGPAGVSQS"
     gene            8177..8995
                     /locus_tag="VHS97_06965"
     CDS             8177..8995
                     /locus_tag="VHS97_06965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3447977.1"
                     /translation="MSIAGIGDRTIALVTGGAQGIGFGIVRSLALASYKVVLADIEVG
                     LLSQAAAELQNEGHEVVGEHLDVTRAEDWGRVVSSLGSRWGQLDLLVNCAGISPRGTV
                     ETTDEALWDRTLAINLKGAWLGIKASISHLRERRGTIINIGSTRATRPMPGLFSYVVS
                     KAGLWGLTQQVAVEYLADGVTCNMIAPGWVDTPNERLIQARHGRPDFPAGIRNLTTPA
                     EIGAAVVYLASAQGRKINGVIHYLDAGLQIADDAGMVYLPDRSRPPYEQRIEGP"
     gene            9055..9867
                     /locus_tag="VHS97_06970"
     CDS             9055..9867
                     /locus_tag="VHS97_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3447978.1"
                     /translation="MSDFVYCLNTSTIRPTPLLEKIAIAGKAGYTAIEPWNDEITAYL
                     EQGGRLSELKRALADSGLKVLSMIALHGWITSEGTEQARVLDDCRRRMAQAVELGSPY
                     IVASPPQCVVDLNRASDRFKELVEIGAQLGVMPSMEFLGFVDGIKNVGSAWAIAAGTG
                     DPRATVVADVFHMMRGGGSIDDLLTLKGDRLACFHINDLPAEPNPLTQKDEDRVMVGD
                     GIADLPRVIANLRTIGYRGPLSLELFNSRLWAQDPLHVATTGLERIRALVEE"
BASE COUNT         1958 a         3074 c         3121 g         2029 t
ORIGIN      
        1 tgagattcac ggagcgaacg gctacttgcc ggaccagttc ctgcaagaca gcacgaacca
       61 tcggacggat gaatacggcg gcccgatcga gaaccgtgct cgcttcctgc tcgatgtgac
      121 ggatgcggcc atctccgttt gggggcccga ccgcgtcggc gtgcacctgg ccccgcgctg
      181 cgacgtgcat tccatgggcg attccgaccg tgcggccacc ttcggttatg tcgcccgtga
      241 gctcggcagg cgtcgcatcg cattcatctg cgcccgcgaa gccctcggtg acgaccgcct
      301 cggccctcaa ttgaaagtcg cgtttggcgg caactacatt gccaacgagc gcttcaccaa
      361 gaagacgggc gaccgggtgc tcgcggctgg cgaagccgat gccgtcgcgt ttggagtgca
      421 gttcatcgcc aatcccgacc tgccgcgccg cttcgcactc gatgcaccac tcaacgagcc
      481 caatcccgaa acgtactacg cgtcgggtcc ggaaggctat attgattatc cgtcgctgga
      541 ggctcaagga gcggcggttt gatactgtcg atcagagcgc cgagcctcac tcgctaaagc
      601 ccggcagcca ccgcagcgca ttctcacgga agatcttgct gagaacttga tcggggagtg
      661 ccagtccctg cgtcgcgtgt cccgcgtagt ccatcttctc gttcccggaa aagaaggccc
      721 agtcgcggtc atgcgtgacc tttagcgccc gcgccgcggt cgcatcgtcg cctgtgcgta
      781 gggaaaaatc ggtggcgtag aggatccggt cttgatagtg agtcaggaac tggcggacct
      841 gatcgcggtc accgcgggcg aagtagcgca cgcgcgcggc tgtatcgacg gcgaagttgg
      901 gataggcgtc gagtcgcttc gccaggcgct tgaggtcgtc ctcatcgcta cccaagtggc
      961 agccgacgac tcgcaactta ggatatcggg cgagtactcg atcgcgggcc gtcaagatgt
     1021 cgtgcttcac cggcgcaccg gccttgccga acatgtgcca ctggggatta ttggagtaat
     1081 acgacaattc tggattcttg gaatccagcg gcagccaggc cccgtccggc tcggcgaggt
     1141 gcgcgaccag cgttcggccg gcccgctcga tcgcctcgta gatcggcagc agggcgggat
     1201 cgtccggcag caggtatgcg cccgacttcg ccttgatcga catgccaata tttttccaga
     1261 tcttgacgcc gatcgcgccg tcctcgaacg atcgacggag gcgggcgatc gttctttcgg
     1321 cgaagttcgg ttcctcgaag ccgcgggcat cgaaggtaga ggcccaggcc agggtaccgc
     1381 cgctgtcgcg cgctaccttg agcgtggcgc cgagcttttc ctccaggtcg aacggctcgt
     1441 caccgctcgc cggacagacg acgatatcca gcccgcgcca gttcgccgcc ttgagggcgg
     1501 acaaaagctc ccgggcatcg cggtggatat gaatatgtgt gtcgatccta ccgaattccg
     1561 gtgcctctcc ggcccgcgcg ttcgtcagca ggaccgcggg aggaatggcg cccaggaaat
     1621 ctcttcgagt caacattctt ccacctcgca ttcgcggtag ttgatttggt cgggacgatc
     1681 ggctggaata cacaaagaga tacgacgcca acctaccccg acacctgccg gattgctatg
     1741 aatccacgag catgcttctg tctgattttg gttgcgaatg ccatattgac catgccggcg
     1801 gccggcgacg acttccccga accgcccaac accgagcgtt cttcgactcg cccattgtcg
     1861 ccggcggatg ccgcggccgg ctggcgtgtc ccggagggtt ttcgggtcaa cgtcttcgcc
     1921 gccgaacccg acgtgaggaa tccggtcgca atggcatggg acacccgcgg gcgactctgg
     1981 attgccgaga attacactta ctccgacacg acccaaaggt ttgacctccg tctccgtgac
     2041 cgcgttttga tattagaaga caacgacgcc gacggccgca tggacaagcg taaagtcttt
     2101 accgacggcg tccagaggct cggcagcgtg gagctcgggt ttggtggcgt ctggctcttg
     2161 tgcccgccgc agttgttgtt cgtcccggac cataacggcg acgacgttcc cgatggtccg
     2221 gccgaggtca tgctcgatgg ttttgcggtc gcaacggata accatcacac cttcgccaac
     2281 ggcctgcgct ggggaccaga cggctggctc tacggtcggt gcggcgcctc gtcaccgggc
     2341 caggtcgggg ttcccggcac gcgcgatgcc cagcgaattc cgatccgcgg cggcctctgg
     2401 cggtaccacc ccatccgcaa gcgattcgaa gtcttgagcc acggcacgac taatccttgg
     2461 ggccacgatt ggaacgcagt cggcgaggct tttttcatca acacggtcaa cggccacctc
     2521 tggcacatga tcccaggcgc ccacttcgcc cgcccgcaca cgatcgaacc gaacccgcgc
     2581 gtctacgcgg tcatcgacca gcatgccgat cactatcact gggacaattc gcggcattac
     2641 aagaacacgc tgattcccgg cggaatcgac gatcatcgtg gcggcggaca tgctcatagc
     2701 ggggtgatga tttacttggg cggccagtgg ccggagtctt ttcaggacaa gctcgtcacc
     2761 ctcaattttc acggcaggcg cgccaacgtc gagcggctcg aacgtttggg cagtggttac
     2821 gtcgggcgcc acgaacccga catgttcttc gcggcggacc cctggtttcg cggcgtcgac
     2881 ctctcctacg gccccgatgg tagcatgtcg gtcctcgact ggagcgacac gggcgagtgt
     2941 cacgagcacg acggcgttca ccgttcttcg ggccgtatct accggttcac ctacgggaag
     3001 ccagcacgcg tcgaccttcg cgatgtgtcg aagctggagg agcgcgatct cgtcgcgctg
     3061 cacctccacg ccaacgaatg gttcgcgcgg caggcgcggc gcgtgctcgc ggagcgatcg
     3121 acccggggcg agccgctctc aacagcgaag gcttcgcttc gcgaaatctt tgagcagacc
     3181 cccgatccct cgcggaagat ccgcgccttg tgttcgctct tcgtgatcgg cgggaccgat
     3241 gcacctttcc tgcgcggctt actacggcac gaacacgaat ccgtccgcgc gtgggccgtc
     3301 cggcttttga ccgacatgct gccgatcgac acgatctaca gccgacgcgg tgggccggat
     3361 gtcgagctgc ccgctgattt gcggagcagg ttcgcggacc tcgccatgac tgatccctcg
     3421 ggcttggtgc gactggtgct tgcctcaacg ctgcagcggt tgccggtagg ccagcgcgag
     3481 tctctcgcac acgctctcct ctcgcggtct gaggacgacg ccgaccacaa tattccatcc
     3541 ttgatatgga caggcctgat cccggttgcc gaggccgatc cagaggcctt ggtccgactg
     3601 gcagtcgact gtcgactcgc acacgtccag cggctgatcg cgcggcggct tggcgaggag
     3661 atcgagacac accccggtcc ggtaaacgcg ctgctcgaag ccgcctcgca acggcccgag
     3721 gggttagtgt tccagacgca gttggtcgcg ggcttaaacg acgccctgac cggctggcga
     3781 aaggcaacca ggcctgctgc ctgggatccg tttcagcaga agctttccac agcggccgac
     3841 ccggccctgc gtgatcggat acgcgatctg aacgtacttt ttggcgacgg ccgcgcgctt
     3901 gaggaagtga agcggctggc gctcgacggc acggcgtccc tcgatgtgag aatgtccgcg
     3961 ctcaagtcgc tgatcgaggc ccggccgccc gacctccgcg ctgtctgcga gcgattggtg
     4021 cgagtgagct tcttgaacgc ggtcgccatg cgcgggctgg cgctctttga cgatccggcg
     4081 atcggtgtga ccttggcgaa gagttaccgc tcgttccatc cgtcggagcg gtccgcggtg
     4141 gttgagacgc tggcttcgcg accggcgttc gccttggctc tacttgacca gatcgcagcc
     4201 ggcgggattc cgcgccagga cctttctgcg ttccacgccc ggcagatccg cggcctaggg
     4261 gcgtcggcga ttaccgagcg tctggcgcaa gtctggggcg tcgagcgtga ctcggccgac
     4321 gaccgccgcg aacaaatcgc gacgctcaag cgcaagctgg gctcatccgc gcttgctcag
     4381 cccgaccgtc gccgcggtcg agccgttttc gatcgggtct gtggcacctg ccacaagctg
     4441 tatggctatg gcggcgagat cgggcccgat ttgaccggag ccggccgaga caatctcgac
     4501 tatttgcttg aaaacctggt cgacccgagc gcatcggtga gcgccgattt ccggatggta
     4561 gtcgtggcta tgaacgacgg ccgcgcgctc aacggtctgg tgcgagcgcg gacggaccgg
     4621 acgttgacct tgcaaacgca aacggcggcc cttgtgctgg accgccagga gatcgagacg
     4681 gttcagccgt cgccgctttc gctgatgccc gacggcctgc tcgccggcct gagcgaaacc
     4741 gagacgcgcg acctgctcgc atacctgatg caccagaccc aggtgccgct accttaggcc
     4801 tcagtccgac cgggatgtac gacgggacga cccgtcgcat ggccgaactt cttggaagcg
     4861 cgatcaacgc cggcggcgag gacggacgat ccgtcggcgc tggtgctcgt ggttcgatct
     4921 cgcccggcca cggcggcgtt ctcagcgaga gagctagatt gggtcggccc cgcggcattc
     4981 atcggaaatc gaacgcagat cgaagtcccc ttgccggcga ccgagtcgac cgagatatcg
     5041 ccttgcattt gtgcgacgag ctcgcggcaa atggctagcc ccaggccgct cccttctatg
     5101 atcgagtgct cgtcgcgatc caaacggaag aaaggctcga aaacacggtc gagcgcttcc
     5161 gcgggaatgc ccggccccga gtcggcgacg acgaccgtcc aggagtcggt gtcgaccttg
     5221 ccggcgacgc gcacctgccc accgactggt gtgtacttaa tcgcgttcga cacgaggttg
     5281 ccgaagattt gctggcaccg cgaggcatcg gtgaccacaa ccatttcggg agcttgggcc
     5341 cgagattccc acaccagttg tctgctttgc gcgaccggcg caaattgacg gtcgagctcg
     5401 tcgatcagag caccgattgt gaatgaaccc aaacagagcg gacgcgatcc ttggacgatc
     5461 ccggcgtaat cgagatagct gcgcgtcagg cgcaagagat cgtcgcagag ggacaccatt
     5521 gtgagcaggt gaccctgctg atccggcgtg atgttcgacg gcgagtcacc caggagcaag
     5581 tcgaaccccg cccggagaga aaccaagggg cggcaaagct cgtggctcaa gttggacaag
     5641 atggatctca gctccccctg atcgatcaag aagcgagcga tctcctgacc ctcgactctg
     5701 ggggaggatg gtgtggaccg gacttccatg gcctcttgtc cctgccgtcg tactttgttg
     5761 gtgcgcaagc cgaccggcgg ggccacgcgc cccgggtctc ggcggtgaat cttacgagac
     5821 gacgatctga ttggccagga gcgggtggcc atcggtcaga tcgagcacgg cttgatgcgc
     5881 aagttgtttt gcgtggtacg tgcgcgaatg tccttggagg atgatcttgt catccgagta
     5941 aacgacatga aggtcacgaa tccgaccgtt ggcgacacgg ttgatgtggc cttcgataaa
     6001 ctcggcgagt tctagattgt ttttgccaaa tccgaaccgc cgcagctcca ttcgagagtt
     6061 gtacatcggc atgccagtct cccacttgat cgactgcacg actggacgac gctttggcct
     6121 atagcgttgc agaacgaaca gattgggagg tcgtctcggg gcgtctttac ctcgagagct
     6181 gggaacgagt tcgactcatc aagttgtgcg catgaaagtc gctcgtacca tccttgatcc
     6241 caaccgcaat ctcatcgacc cggaatctgg aaatctgtcc agtccgcagc tcgagaaaat
     6301 ccatctcaag cttgaccgga ctgagtgaga ctaccgactg atcgcgccct agtcaaacat
     6361 tattcgccct gggggagctc aaaatggcgg cgcaagggcc gcgatgccga gggaggaggg
     6421 ggttggtcct gagcctcgaa cagttcgtcg gtctggatga tggactcagc gagtttcgcc
     6481 atcgggatcc ttcgatcctg gctttgacgc tgtagcagcc ggaaggcttc gctctcgctg
     6541 agctgcttgc gcgtcatgag aattcccttg gcacgttcga tcagcttgcg ttcgcgcaga
     6601 gtcgatttga gctggtcgac gtcgtcttgc aatagcttga gctcgcgcgc tcgtgccacg
     6661 gccacctgaa tggcggcgtg caggctgggg ttagtcgccg gtttgagcaa atacgagatc
     6721 accccgtcct caaccgcctg gtcgatcaag ttgggatggc cgtgcgcggt caggatgatg
     6781 accggagtac ccggatccgc agcgatctga cgcgcagcgg aaaggccatc cagacctggc
     6841 atctcaatgt ccatgaagac gacgtccgga atactccggc ggcacatctc gatcgcgcga
     6901 tggccatcgc tgaccgcggc aaccacgtcg tatcccaacg attcgagctg attttgtagt
     6961 ccgaccgcga ctgttttttc gtcgtctgcg atcaaaatac gatagggccg agtcatcgcc
     7021 gtaatccctc aaagcacaat cagagtgttg accgactttg tcacgcagtt ccgatgatgt
     7081 gcagttctaa tgccgtttca tcgaaatctc acaagagatc taaaagtcat cgagctgacg
     7141 cttctcagct ggtattgcgc tctcaatcaa acaccaacca ttcagctcgt ggaaacatga
     7201 tatcctacga tatcaaacac tcttgacggc atctcgtcgg cggtcagaat gttgctggag
     7261 aagccgtcct ggccaaacga tttgaccgag aatcgccgat tgattcctaa acaagtaatt
     7321 acggaggatt tgcaaggtcg tgactcgcca cccatggttg atctggtcat tcgatgatct
     7381 cgtggtcggt gacgagtggg aaagcccacg acggaccgtt acggagaccg atgttgtact
     7441 attcgccggt ttgtcgggag acttcaaccc ccttcacgtc gatcattcgt cttttcgaag
     7501 cagcccattc gggcggccgg tggctcatgg cctgctcggc ctggccattg caagtggttt
     7561 gatgagccag gcgccgcgag tcgacaccct cgcatttctg gcgatcctcg aatggaagtt
     7621 tcataagccg atactttttg gcgacacgat ccacgcacgt tacactatcg agtcgttgca
     7681 atctcaagcg aaagggcgta ggggcgtagt cacatggcac cgacgaatct ttaatcaaga
     7741 tgataatctg gttcaagaag gccgtaccca aaccctcgta cgagctcgga cgcgatcccg
     7801 ttcgagcaaa cgcaccgaat ccgacggccc agcgggtgtc agccaaagct gatcgtacca
     7861 ggtcacgagt cgcgcgacat cgtaccttat accatcgtag tcctaataaa ttcgatgaca
     7921 aataaccgca aaaatattgg aattgcagca attcggccgc aattgcatct ttgctcctca
     7981 tcatcgtgac acgcagctac gcctcaatcc aaggcatggc tttttgggcg atattccgat
     8041 ctgagaggtt tttaatagca gtgtttcgct gcccactggg gggcaatgcg attgctttgg
     8101 cgaactgggc ttttgctgac gatccgttgt gcgcgcgcag gaatgcgtgc gagagtgtcc
     8161 cagggagatt gaaactttga gcatcgccgg aattggagat cgaacaatcg cgcttgtgac
     8221 aggcggagca caaggaattg gcttcgggat cgtccggtca cttgcattag cctcgtacaa
     8281 agttgtcctc gctgacatcg aggtcggatt gttgtcgcag gctgcggcgg aattgcaaaa
     8341 cgaaggtcat gaggtggtcg gcgaacatct ggatgtgacc cgagccgaag actggggccg
     8401 cgtggtgtcg tccctcggtt ccagatgggg acagctcgac ctgcttgtga attgcgcggg
     8461 catcagtccg cgagggacag tggaaacaac cgatgaggcc ctctgggatc gaacgctggc
     8521 aatcaatctc aaaggagcct ggctcggcat caaggcatcc atttcccact tgcgcgagcg
     8581 tcggggtacg atcatcaaca tcggttcgac gcgggcgacg cgtcccatgc ccggtttatt
     8641 ctcttatgtg gtcagtaaag ccggtctctg gggtttgacc cagcaggtgg ccgtcgagta
     8701 ccttgcggac ggagttacgt gcaacatgat cgctccaggc tgggtcgaca cgcccaacga
     8761 gcgtttgatt caggcgagac acggtcgtcc cgatttcccc gcgggcattc gcaatctgac
     8821 gacgcccgca gaaatcggcg cggccgttgt gtacctcgcg tcggcccagg gtcgtaaaat
     8881 caacggcgtc atccactacc ttgatgcggg tctgcagatt gccgacgatg cggggatggt
     8941 ctacctgccc gaccggagtc gaccacccta cgaacaacga atcgaagggc cctgatgtgt
     9001 ccgccgaccg atgggtgggc ccatgctgac ggcaagccgg ctggagagag aaaaatgagc
     9061 gactttgtct attgcctcaa cacgagcacg atccggccga cgcctttgct cgagaaaatc
     9121 gcgattgccg gtaaagctgg atacaccgcg atcgagccct ggaacgacga gatcacggcc
     9181 tatctcgagc aaggtgggcg cctctctgaa ttgaaacgag cgcttgcgga cagtgggcta
     9241 aaagtgctca gcatgatcgc acttcatggg tggatcacgt cggaggggac tgagcaagcg
     9301 cgcgttctcg acgattgccg acggcgcatg gcccaagccg tcgagctggg aagtccctac
     9361 atcgtcgcca gtccaccgca gtgtgtcgtt gacttgaacc gtgcgagcga ccgttttaaa
     9421 gaactcgtcg aaattggtgc gcaactcggc gtcatgcctt cgatggagtt tttgggtttc
     9481 gttgacggga tcaagaacgt tggcagtgcc tgggcgatcg cggcgggcac gggcgacccg
     9541 cgtgccacgg tggtggctga cgtctttcac atgatgaggg gcgggggctc gatcgacgac
     9601 ctcctcacgc tcaaaggaga tcggctcgcc tgctttcata tcaacgactt gccggccgag
     9661 ccaaacccgc tcacccagaa agacgaagac cgggtcatgg tcggcgacgg catcgccgat
     9721 ctgccgcgcg tgatcgccaa tctgcgcacg atcgggtacc gcggcccact ctcacttgag
     9781 ttatttaaca gtcggttatg ggcacaggat ccgctccatg ttgcgacaac ggggctagag
     9841 cggatccgag cgctggtcga ggaatgagat cgagacgcca tacccggact cgaagctggg
     9901 acgtacccgg ggcaatttct gcgcgagcgc ggagtggcac gggcttccag cccgtggctg
     9961 aaaatctcac gggcaagatg cccgtgccac gtacgccagc cgggccgctt gctcaactta
    10021 ttctttcgcg gtgccttagt tagtagttgg caacgttacc cgatgcgtct tgcagattga
    10081 gttggcgtac gatatcggcc gagccgtcga gcatcatttt gagctcgctc acgaccgcga
    10141 tgaactgcca gccttcgcgg gcacgccgca tcgcgtcggc gg
//
LOCUS       DATAQL010000404         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4925198, whole genome shotgun sequence.
ACCESSION   DATAQL010000404 DATAQL010000000
VERSION     DATAQL010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4925198"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1814)
                     /locus_tag="VHS97_06975"
     CDS             complement(108..1814)
                     /locus_tag="VHS97_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447979.1"
                     /translation="MIGRAALLAIALAAAPPQESGPAATGNEAGHYLVFLAENRPVFL
                     RLRIAVHDRPFEASWIDSVRMLHASLDRNGDGTLTTKEADPNVFAALVRLATGVAAPP
                     AHGELDVHPKDGVVSFDELAEALRLILGPFRLQVGRQAIGRTDALFEQLDRDKDGQLT
                     RPELAAIAGSLRPLDLDDNAMISDYELEPFNSPAVTAIATESSERRARFTALPPAVEL
                     VPGESSLRSARLLLKKYDKGKGDQPGRPDNKLSPSEFAIDPDAFARADTNGDDAIDTE
                     ELRKFLARTPVDLTLDVTLAPDASGRATIRTGAGGVMPKGAQLRQLGDGDVEFAIGQV
                     RLDIHVEDSNTTGEEARRILEQRFKAADANKDGYLEGKEQASINGPQSPLAGLAGVID
                     RDSDGKIYLKELLEFANRQTEAARCRLVATTADQGRAIFGILDLDRDRRLGAREVMRT
                     VDRVTSWDSDGDGRVSADEIPYHFQVTIARGGLPGLTGAGVATPATRTMVGAGSAVGP
                     DWFQKMDRNHDGDVSRREFLGPRDQFDRLDRDKDGLIDPDEARTAAAAKTKETQRDGS
                     HP"
     gene            complement(1811..>2990)
                     /locus_tag="VHS97_06980"
     CDS             complement(1811..>2990)
                     /locus_tag="VHS97_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447980.1"
                     /translation="PSQTDTFDPKPGHANSGPFKPIKTAVPGMLFGPHLPKLALQAKD
                     IAVIRSMSSKEWDHGRATYYLRTGYLPSGPVRHPTLGSLVANEFDDDSAELPGFVSIS
                     PVRAINAAAFSSGFLGPKCAPLNVGERSLAGGADAGSPSLRVEDLEVPRAIGRARADE
                     RLELMHTLGSDFLTNRPGVPSLSHQSAYVRAVKMMRSSAAQAFELDQEPAAVQDAYGR
                     NPFGQGCLLARRLVERGVPFVEVTLSSVDGGNSLGWDTHAQNFDAVEKLSGVLDSGWS
                     TLMTDLRTRGLLESTLIVWMGEFGRTPKINESAGRDHFPNAWSTVLAGGGIRGGQMIG
                     DTGVDGEEVKDRPVLVPDLLATVVKGLGIDPSKQNVVDNGRPIRLVDPKANPIKELLG
                     "
BASE COUNT          510 a          917 c         1003 g          560 t
ORIGIN      
        1 acgtgcgcaa cccctaaagt cggactttca ggccaaagcc atggaattgg cacaacagcg
       61 tcggagtata atgatatgta ttttcctcct acgtcatagt ggagggctca ggggtggctg
      121 ccgtcccgct gcgtttcctt ggtcttcgcc gccgcggcgg tgcgggcctc gtctgggtca
      181 atcaggccgt ccttgtcgcg gtcgaggcgg tcgaattgat cgcgagggcc caggaattcg
      241 cgccgggaga cgtcaccatc gtggttgcga tccatcttct gaaaccaatc cggaccgacc
      301 gcggagccgg ctccgaccat cgttcgagtg gcgggagtgg ccacgcccgc acctgtcaga
      361 ccggggagcc cgccgcgggc gatcgtgacc tggaagtgat acgggatctc gtcggccgaa
      421 acgcggccgt cgccgtcgct atcccacgag gtgacccggt cgacggtgcg catcacctca
      481 cgggcgccaa gccgacggtc gcggtccagg tcgagaatgc cgaagatggc gcggccctga
      541 tcggccgtcg tcgcgaccag ccggcagcgc gcggcctctg tctggcggtt ggcgaactcg
      601 agcagttctt tcaagtagat cttgccgtcg ctatcccggt cgatgacccc cgccaaacct
      661 gcaagtggcg actgaggtcc attgatcgaa gcttgctcct tgccttctaa gtagccgtct
      721 ttgttggcgt cggcggcctt gaaccgctgc tcgaggatgc gacgggcctc ctcgcctgtg
      781 gtgttactgt cttcaacgtg gatgtcgagc cgaacctgtc cgatagcgaa ctcgacatca
      841 ccgtcgccaa gttgccggag ctgggctccc ttgggcatga cgcctccggc gcccgtccgg
      901 atcgtcgctc ggccggaagc gtcgggcgcg agggtgacgt cgagggtgag gtcaaccgga
      961 gtccgagcga ggaattttcg tagctcctct gtgtcaatcg catcgtcacc gtttgtatcg
     1021 gcgcgcgcga acgcgtccgg gtcgatcgcg aattcgctgg gcgagagctt gttgtcgggc
     1081 ctacccggtt ggtccccctt ccctttgtcg tactttttga gcaagagtcg ggccgaccga
     1141 agtgatgatt cacctggcac aagctcgacg gccggcggga gggcggtgaa gcgagcccgg
     1201 cgctcggacg attcggttgc aatcgcggtc accgccgggc tgttgaacgg ctcgagctcg
     1261 tagtcgctga tcattgcgtt atcgtcgaga tcgagcggtc ggagcgagcc ggcgatggcg
     1321 gcgagttcgg gtctagtgag ttggccgtcc ttatcgcggt cgagttgttc gaagagcgca
     1381 tcagtgcggc cgatcgcctg ccgcccgacc tggaggcgga agggacccaa aatgaggcgg
     1441 agggcctcgg ccagttcgtc gaacgagacg acgccgtctt tggggtgaac gtccagctct
     1501 ccgtgagcgg ggggcgcggc cacaccggtc gccagccgca cgagcgcggc gaaaacgttc
     1561 gggtcggctt ccttggtcgt gagagtgccg tcaccgttgc ggtcaaggct ggcgtggagc
     1621 atccgcacgg aatcaatcca cgacgcctcg aagggtcgat cgtgtaccgc gatccgcagg
     1681 cggaggaaga ccggtcggtt ctccgcaagg aagaccagat agtgcccggc ctcattcccg
     1741 gttgcggccg gcccagattc ttgtgggggc gcggctgcaa gggcaattgc caacaaagcc
     1801 gctctaccga tcatcccaga agttccttga tcgggttggc cttcggatcg acgagccgga
     1861 tcggccgacc gttgtcaacg acgttctgct tggatgggtc gatccccaga cccttgacga
     1921 cggttgccag caggtcgggg accaggacgg gccggtcttt gacctcctcg ccgtcgacac
     1981 cggtgtcgcc gatcatctgg ccgccacgaa tcccaccgcc ggcaaggacg gtggaccagg
     2041 cgttggggaa atggtcgcgg ccggcggatt cgttgatttt gggggtgcgg ccgaactcgc
     2101 ccatccagac aatcaaggta ctttcgagca agccgcgggt tcgaaggtcg gtcatcaggg
     2161 tggaccaacc agaatcgagg actccgctga gcttttcgac ggcgtcgaaa ttctgcgcgt
     2221 gagtgtccca gcccagcgaa ttgccgccgt ccacggacga gagagtcacc tcgacgaaag
     2281 gtacgccccg ctccaccaag cgacgggcca gcaggcaacc ctggccaaac gggttccggc
     2341 cgtacgcgtc ttgcacggcg gcaggttctt gatcgagttc gaacgcctgg gcggccgacg
     2401 acctcatcat cttcacggcc cgaacgtaag cactctggtg actgagcgac gggacgccag
     2461 gccggtttgt gagaaagtca cttcctaacg tgtgcatgag ctcgagccgt tcgtcggccc
     2521 tggcgcggcc gatggctcgt ggtacctcaa gatcttcgac ccgcaacgac gggctccctg
     2581 catccgcgcc acctgccagg ctacgttcgc cgacgttcaa cggtgcacac ttgggcccga
     2641 ggaagccgga gctgaacgct gcggcgttga tcgcccggac cggcgagatg cttacgaacc
     2701 caggcagttc ggccgaatcg tcgtcgaact cgttggccac gagtgagccg agcgtgggat
     2761 ggcggaccgg cccagagggc aggtagcccg tacggaggta gtaagtggct cggccgtggt
     2821 cccattcctt ggagctcatc gagcggatga cggcaatatc cttggcctgg agagcgagct
     2881 tcggcaaatg cgggccgaaa agcatccccg gcaccgccgt cttgatcggc ttgaacgggc
     2941 cgctgttagc gtggcccggc ttgggatcga acgtgtcggt ctgactgggt
//
LOCUS       DATAQL010000405         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2024708, whole genome shotgun sequence.
ACCESSION   DATAQL010000405 DATAQL010000000
VERSION     DATAQL010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024708"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VHS97_06985"
     CDS             complement(<1..259)
                     /locus_tag="VHS97_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3447981.1"
                     /translation="MEMDPRESSPLPMGLSRRRWLQRAGGGMGALALDWLMREEQARG
                     EGRPDGPLAPHQPHFRARARSVIYLFMHGGPSHLETFDPKPE"
     gene            complement(535..2268)
                     /locus_tag="VHS97_06990"
     CDS             complement(535..2268)
                     /locus_tag="VHS97_06990"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX3447982.1"
                     /translation="MPARRGAIHVATTKRVYKGKTYVTHLLRRSIRKGATVTHETLGN
                     LSHLPDHLIDIIKRSLKGETFVPAAETFRTTRTLPHGHVEAVLTMIRKLGLEDLIASK
                     PSRQRQLVFAMITERLLFPSSKLANTRHWRDTTLAEELDVVDATEHDLYDAMDWLLAR
                     QGAIEKKLARRHFGDGALVLYDVTSSYYEGKTCPLARFGHDRDGKTGRPIIVYGTLTD
                     ADGRPVAVQVYPGNTADPKTVPDQVETLTKRFGLSRVVLVGDRGMLTQTQIDTLKKHP
                     GLGWVSALRSGAIRRLLADGILIRETLETERLAEITSPEFPGERLMACYNPQLAEQRR
                     HKRQELLAATEAQLKALAASVARPRGPLQKAAEIGVRAGKIINHYKMAKHFTLTIRDQ
                     GLEWARNRDAIQKEELLDGIYVIRTSEPAERLSAADGVRSYKRLTLVEQAFRCLKGLD
                     LLVRPIYHHTAERVRAHILLCLLAYYVEWHLRQKWEPLLFEDEELALDRNRRDPVAPA
                     KASKSALQKKKTKLNPDGLPVHSFRTLIAHMGTRCRNTCVVTSDPKQTPFFQVTDPDA
                     LQAEALRLIMM"
BASE COUNT          520 a          751 c          780 g          576 t
ORIGIN      
        1 gctcgggctt ggggtcaaaa gtttcgagat gactcggacc gccgtgcatg aacaggtaga
       61 tgacgctccg cgcccgagct cgaaaatgag gctggtgagg cgcgagcggc ccgtcaggtc
      121 ggccctcgcc ccgcgcctgc tcttcgcgca tcaaccaatc gagcgccagc gcgcccattc
      181 cgccgcccgc tcgttgtaac caccggcgcc gtgacaaacc catcggtagg ggagaagact
      241 ctcgcgggtc catttccatc tcggtgaaaa ccacgcttgg tctggaatgt cgacttattg
      301 tacttgcacg gacggatact cgctagccaa tgcccgagac aaccaaagcg gacccttcaa
      361 acacggtccg aacggcgaaa cggcgacgac cagcaagttc agaacgcggg gcctgcatag
      421 ttacaatcta cgtaaggaat gaggaccgag gattaatctg aagttccagg gacggaaagg
      481 aggaatctct attgtgataa attacttacg tcaattccag tttcccgttt ctggctacat
      541 catgatcagt cggagcgctt ctgcctgcag cgcgtcgggg tccgtcacct ggaagaacgg
      601 cgtttgtttg gggtcgctgg tcactacaca cgtgttacgg caacgcgttc ccatatgagc
      661 tatcaatgtt cgaaaactat gaaccggcaa accatccggg ttcaacttgg tctttttctt
      721 ctggagcgcc gacttcgagg ctttggcagg cgccacgggg tcgcgtcggt tccggtccag
      781 agccagttcc tcgtcctcga acaacagcgg ttcccacttc tgccgcaagt gccactccac
      841 ataataggcc agcaggcaca gaaggatgtg cgcccgcact cgctcggccg tgtggtggta
      901 gatcgggcgc accaaaagat cgagcccctt gagacaccgg aatgcctgct ccaccagggt
      961 cagccgcttg tacgatcgca cgccgtccgc ggccgacagc cgctcggcgg gctcgctggt
     1021 ccggatcaca tagattccat ccagaagctc ttctttttgg atcgcatccc tgttgcgagc
     1081 ccactccagg ccctgatcgc ggattgtcag ggtgaaatgt ttggccatct tgtaatggtt
     1141 gatgatcttg cctgctctca cgccgatctc ggccgccttt tggagtggac cacgcgggcg
     1201 tgccacgctc gcggccagtg ccttcagttg ggcctccgtg gccgcaagca gctcctggcg
     1261 cttgtgccga cgctgctcag ccaactgggg gttgtagcaa gccatcagcc gttcgccggg
     1321 gaattccggg gaagtgattt ccgccaggcg ttcagtctcc agagtttccc tgatcagaat
     1381 gccgtcagcc aacaggcggc ggatggcgcc gctgcgcaac gccgaaaccc agcccaatcc
     1441 cgggtgtttc ttcagtgtat cgatctgagt ttgcgtgagc atgccacgat caccaaccag
     1501 caccacccgc gacagaccaa accgtttcgt gagcgtttca acttggtcag gaaccgtctt
     1561 ggggtccgcg gtattgcccg gatagacctg cactgccact ggccgtccat ctgcgtcagt
     1621 caacgtgccg tagacgatga tcgggcggcc ggtcttgccg tcgcggtcgt gaccaaagcg
     1681 ggccaggggg caggtcttcc cctcgtagta gctgctggtg acgtcgtaga gcaccagcgc
     1741 gccatcgccg aagtgccttc gggcaagttt cttctcaatc gccccctgcc gagccagcag
     1801 ccagtccatg gcgtcataga ggtcatgttc ggtggcgtcg acgacatcca attcttctgc
     1861 caaggtggta tcgcgccaat gtcgggtgtt ggccagcttg gagctgggga agagaagtcg
     1921 ctccgtaatc atggcgaaga cgagctggcg ttgccgcgaa ggcttggagg cgatgagatc
     1981 ctcaagtccg agcttgcgga tcatcgtcag gacggcctca acatggccat gcgggagcgt
     2041 gcgagtggtc cggaaggttt cggcggccgg gacgaaggtc tcgcccttga gggagcgttt
     2101 gatgatgtcg atgaggtgat cgggaagatg ggaaaggttg ccgagggtct catgcgtgac
     2161 ggtcgcaccc ttgcggatgg atcgccggag caaatgggtg acgtaggtct ttcccttgta
     2221 gaccctcttg gtcgtggcca catgaatggc gccgcgtcgt gctggcattc ggccattttc
     2281 taagggcctt ggttcgtgtc aagagaatct agacattttt agtggctaca attcgaaacc
     2341 gaaatcgaga ctaacaactg cgaggcgaaa gatttagggt aaaaaacggc ccggaacttc
     2401 agtctagaag ccgctctcgg cccggacgcg cagacttggt tgaacctgga gacaagctgg
     2461 cggcttgtag ccgtatgatg ttacctaata ttatttgtaa attacgtttt cgtatttctc
     2521 tgtgtctctg tggtgagcgt tttttccgat catacccgtg gtgaatcacg accatgaatt
     2581 gcgacgttat ccgcgggctt acatcggtca tcgtcccttg cttgaat
//
LOCUS       DATAQL010000406         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4009389, whole genome shotgun sequence.
ACCESSION   DATAQL010000406 DATAQL010000000
VERSION     DATAQL010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4009389"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..436)
                     /locus_tag="VHS97_06995"
     CDS             complement(29..436)
                     /locus_tag="VHS97_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447983.1"
                     /translation="MSLCRTRPIVRLGLFLAAASFAPAPPEKPATVSPLAEARYRAAV
                     KQFDETWAYYRQARIDSYQVYVWSRLVLESRRAIAVKPAEQITALEDHLDRMKKLEEL
                     VTKIRRLGFGRSYDVGASEYYRLEAENWLEQAK"
     gene            complement(468..1304)
                     /locus_tag="VHS97_07000"
     CDS             complement(468..1304)
                     /locus_tag="VHS97_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HEX3447984.1"
                     /translation="MQMLIIGLIVLSIYLFLRLLAKFGAWMSGARFRSYRQLAARYNG
                     RYESRGLSDSPTVSFTHNGSTVRVGLAPTIAGQPEQIPRTRVVARFARGIPFRLELAP
                     AARPAPHQPPKGTRLVKVDDPDFDRGFVVQANDHDIARDFLSPNARRVINTLQKGVHA
                     GGMLVSINPERILVQIDRNLGLKTEALAWAVQNALVLHDGLIEGVSRRITQGIAIVDQ
                     PDDAWDEDEGPPICKVCGEPIAGGAVIVCAVCGTPHHRDCWEYVGACSIYGCDGKVGE
                     LG"
     gene            complement(1441..2331)
                     /locus_tag="VHS97_07005"
     CDS             complement(1441..2331)
                     /locus_tag="VHS97_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX3447985.1"
                     /translation="MSRMNADRGEIGLTFEEHGSPSLWNWQPGQDVGEIMPNEIRVWI
                     VDLDAGLNVDDVETAEPGPESSLLSPDEQERAARFVRARDRRRFVCCRAALRSILGGL
                     LRQPPDSLRFRAAVRGKPELDRDSSGENHADKLPALCFNVSHSSELALIGVCRGYELG
                     VDLERVRTIHEADRIVESFFSRAEHAEFATISDDLKPLAFFRGWTRKEAILKGLGIGL
                     AGLSARYETGFGTSDLAPHFAPATPVALVDDWRLWEACPRADFVATVAVRAPIFPYTP
                     PEGAGTEGTVVASSGEDPVH"
     gene            2434..2712
                     /locus_tag="VHS97_07010"
     CDS             2434..2712
                     /locus_tag="VHS97_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447986.1"
                     /translation="MAILFVLVGFVTAGCIDVYFFGIGKPGGFDGLGPVGASVAESAC
                     GLIFGAVISIALYLNSELPMRHSEQVCPTDASCGPIVWHRLDDPRLGP"
BASE COUNT          546 a          908 c          877 g          561 t
ORIGIN      
        1 ataactgcgc agactaccaa gaagaccatt atttggcttg ctcgagccag ttctcggcct
       61 cgaggcggta atattccgac gcgccgacgt cgtaagaccg gccaaatccc agtcgccgta
      121 tcttcgtgac gagctcttcc agctttttca tgcggtccag atgatcttcg agcgcagtga
      181 tttgctcggc cggtttcacg gcgatggcgc gtcgagattc gagaacgagt cgggaccaga
      241 catagacctg atacgagtcg atccgcgctt gccgatagta tgcccaagtc tcgtcgaatt
      301 gcttgacggc cgcgcgataa cgcgcctcgg caaggggaga aactgtggcc ggtttctccg
      361 gcggcgcggg ggcaaacgac gctgccgcta agaataggcc cagccgaaca atcgggcgcg
      421 ttctgcacag tgacatgatc ccctccctga ccgctctagc cacagcgtta accgagctca
      481 ccgaccttgc catcgcagcc gtagatcgaa catgcgccca catattccca acagtcgcga
      541 tgatgcggcg tgccgcagac cgcacagacg atcactgctc cgccggctat cggctcgccg
      601 cagactttac atattggggg gccttcatcc tcgtcccagg cgtcgtcggg ctgatcgacg
      661 atggcgatgc cttgtgtgat ccgcctggac accccttcga tcaatccgtc gtgaagtacg
      721 agcgcgttct gcaccgccca ggccagggct tcagtcttca ggcccaggtt acggtcaatc
      781 tggacgagta tccgctctgg gttgattgac acgagcatcc cgccggcatg gacgcccttt
      841 tgcagggtgt taatgactcg ccgcgcgttg ggcgagagga agtcgcgcgc tatgtcgtga
      901 tcgtttgcct gaaccacgaa gccgcggtcg aaatcaggat cgtcgacttt aaccagccgc
      961 gttcccttgg ggggctggtg aggggcaggg cgggccgccg gtgccagctc gagacgaaac
     1021 ggtattcctc gagcgaaccg cgcgaccacg cgggtgcggg gaatctgctc gggctgaccg
     1081 gcgatcgtgg gagccaggcc gacacgaacc gtcgacccat tgtgggtaaa gctgacagtg
     1141 ggcgagtcgg atagcccgcg gctttcgtac cgcccattgt agcgtgcagc cagttgccgg
     1201 taggaacgaa accgcgcgcc gctcatccac gcgccgaatt tggccagcag gcggaggaat
     1261 aagtagatgg acaagacgat caaaccgatg atcagcatct gcatcggctc atatccctcc
     1321 acgctcctcg cgagccgtgg cggcccaaca cgaactgccg ccggaatgga cgcacccttg
     1381 ccaatcgggc gaggccgcgc gattcctcac cacagttcgc cttctttgct cgccgttcct
     1441 tcagtgtacg gggtcttcgc ccgaactggc aacgacggtc ccttcggtgc ccgcaccttc
     1501 cggtggtgta tagggaaaga tgggggctcg aacggccacc gtcgccacaa aatcggcacg
     1561 gggacaggct tcccagagcc gccaatcgtc caccaaagca accggtgttg ccggtgcgaa
     1621 atgaggcgcc agatcgctgg ttccaaaccc cgtctcgtac cgggccgata gaccggcaag
     1681 cccgatcccc aatcctttta atatcgcttc tttgcgggtc cagccgcgga agaaggcgag
     1741 tggtttcaaa tcgtccgaga tggtggcgaa ttcggcgtgc tccgcccgtg agaagaacga
     1801 ctccacaatg cgatcggcct cgtggatcgt ccgcacccgc tccagatcga caccgagctc
     1861 ataaccgcgg cagacgccga tcaaggcgag ctcggacgag tgcgacacgt tgaagcaaag
     1921 ggcgggcagt ttgtcggcgt gattttcgcc ggagctgtcg cgatccagtt ccggcttgcc
     1981 acgcacggcc gcacgaaacc gcaacgagtc gggtggctga cgaaggagac cgcccaggat
     2041 cgaccgcagc gccgcccggc agcaaacgaa tcggcggcgg tcgcgcgccc gcacgaatcg
     2101 ggcggcccgc tcctgttcgt ccggtgagag caagctagat tcggggcctg gctctgccgt
     2161 ttccacgtcg tcgacgttta gaccggcgtc cagatccacg atccagacgc ggatctcgtt
     2221 cggcatgatc tcgccgacgt cttggccggg ctgccaattc cagagcgagg gggatccgtg
     2281 ttcttcaaac gtcaggccaa tttcgccacg atcggcgttc attcggctca tcacttgttg
     2341 attccttctc gcgctttatt cgtctcgtcc ttgacatggc ccaaccaaac gatcgacact
     2401 ggctgtaccg gcgcggggat cctgggtgcc gcgatggcca ttcttttcgt cctggtaggc
     2461 ttcgtcaccg ccggctgcat cgacgtttat ttctttggta tcgggaagcc gggaggcttt
     2521 gacggcctcg ggccggtggg tgcctcggta gcggaatccg cgtgcggtct gatctttgga
     2581 gccgtgatta gcattgcact ctatcttaac tccgagctgc caatgcgaca tagcgagcag
     2641 gtctgtccaa cggacgcctc ctgcggtccg attgtctggc accggcttga cgatcctcga
     2701 ctcggccctt gatttcgagt gagacaggtc aatgaaagcc atcacaatca ccgggaccgg
     2761 cggcccggaa gttctgcaca tccgcgagat acccacaccc gagccaaggg gcgagcaggt
     2821 gcgcgtgcgg gtgcgagcct gcggcttgaa ccgggccgac cttatgcaat gccggggcta
     2881 ttatcccgct cc
//
LOCUS       DATAQL010000407         7131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2025139, whole genome shotgun sequence.
ACCESSION   DATAQL010000407 DATAQL010000000
VERSION     DATAQL010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025139"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1318)
                     /locus_tag="VHS97_07015"
     CDS             complement(26..1318)
                     /locus_tag="VHS97_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEX3447987.1"
                     /translation="MMKLPGPRDRSSVSIILAVIANLIGAMVECRADDRFIPLALKSQ
                     ITRVQPMTGIVLWTTSEHRITDAVQLEYSYMKYGDVVTGRDQYDWRVMDRLLEDVASR
                     KHQAIVRFYFVYPGHETTVPAYIKGLSDYHELPSKSEGKPTTFADWSHPELKRFTLAF
                     YEKLAERYDQDPRLAFLETGFGLWAEYHIYDGPMKLGKTFPDKPFQAEFARRLARAFQ
                     KTPWMISVDAADPSRAPFAADGELLRLPFGVFDDSFLCRQHARENEPNWNAVGRDRWK
                     RQPAGGEFSYYTRADQKQALAASGPHGISFEKAAADFHLTFMIGNDQPRYQPLDRVRT
                     AGLACGYKFRILAFEASPSNARITITNTGVAPIYHDAFLTVNRVRSARTLKGLLPGES
                     RTDEIAAGGESPKLTIESDRLVAGQAIDFEADLKSDSR"
     gene            complement(1363..1926)
                     /locus_tag="VHS97_07020"
     CDS             complement(1363..1926)
                     /locus_tag="VHS97_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX3447988.1"
                     /translation="MVNWSSHFESALPYDKFLDQYATPSQRARWDAMHARFALTSAQR
                     ELISSFARRMPVLCVAGAWCGDCINQCPVFDHFAQASSRIELRFLDRDAKPELRDLLA
                     INGGHRVPVVAFLSEDWFEVCRYGERTLSIYRRMAADQLGPACPTGLVPPADEALAVN
                     TAEWLAEFERAQLILRLSPRLRSKHGD"
     gene            complement(2006..2494)
                     /locus_tag="VHS97_07025"
     CDS             complement(2006..2494)
                     /locus_tag="VHS97_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447989.1"
                     /translation="MQRGRLILDVSVADLEAAEAKFGAFHHTTWHFRNALAEAQRSWD
                     RLGAELGAPALEAALNLPPLTTLTLGERAKGVAPVVLILIAGQTYRSQRVQGTELAPI
                     QWRLTRLNPPLENGPYYACRLASGSTQCDCADWTYQIAETENTRRKHCKHLQALATLG
                     WI"
     gene            complement(2838..4778)
                     /locus_tag="VHS97_07030"
     CDS             complement(2838..4778)
                     /locus_tag="VHS97_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447990.1"
                     /translation="MEPDLSLRRRREPPVAWDHGRSVAPSGSGEALRPMDRPVIRPAP
                     RSAMVFPLVVLIAVLPGMVALNAWDLTPPGPLWGLRGLAVLDGLVLDQMPAAAEIKSV
                     PESAAFSSVAFQPPLYAWLEAIGFWLSNDRDPLASVLPSYIAGALVVGLVYLHGRLWR
                     GAGLGLTAAILVGFNQNLLLRMQEATPATLVVCGVLAALLAYGWHERMAALSVRPWSW
                     SGPVVWAAVGGIALGAALLSLGGLALIVIPIVLLHQFYLSPSMASSSQHSLPRFWWRG
                     RRDSPGIVDGLLALSIALAVALPWYVLMVKSYGWQALFALRAPPDSLAGDVHTGLLPR
                     LIKLAPVTLPLALFGAVRAIRSALVDESETRETVGGSFWVVWLAVAALAPAIWPGGPR
                     RAFDLLLLVPVSLLAAQTIADLANRRLSVRALILLAPATAMSIAWWASADLNRAVEDV
                     IHGRADTATALGLHLAFDLVVVSVLLIRGLYRWARRRDDRQRGILAVFLLSIVVVTVV
                     VGLLEGLFRHSETHALLALRTMILRRNRENPFQVVAVVSPSVFERTANGGGPAGDRPL
                     PGGRLRFILRTALPRVAQRDLNAIDGLFNLPDGQRLVVLAGTGKRLSSADQSRLGLEA
                     IHPGRSGILDAYATARNRLTRR"
     gene            complement(4898..6112)
                     /locus_tag="VHS97_07035"
     CDS             complement(4898..6112)
                     /locus_tag="VHS97_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447991.1"
                     /translation="MTPALERLAELVRQAEAKTRARKIEGETQHAAELFRAVELRARR
                     AVEQLRALRPTSLRALEEANADEQLLKDLVKKLALYKSSLGSEADAERLIATSQAEIE
                     RIRCQTKADLEQIGHDVEEARRELRTATDHYRQLRRELDRLQPELAAQFAADDRLLWD
                     AEAHFPGGQLQLLAHEVEVGLGAFAGLNKLEQYARLKVWIGRFRFHQASQDRDALVTE
                     ELQALSHKVFHQLKWLSRQYEPGYIEAFRQDFSTDWATYVAEAQEQLVQAIEAGRRAR
                     DAEQPRIRESSDDQPSTSGVAQASRLAAPTSTATLSAAPPAYSHAAPPNLNGLKSLVS
                     RLHLPDEGLDQFLAALRHVIDDVGTANPDLLRLVMPYREYITGDDGLGALRRNLERIQ
                     ARRDDESAHHDA"
     gene            6480..6707
                     /locus_tag="VHS97_07040"
     CDS             6480..6707
                     /locus_tag="VHS97_07040"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEX3447992.1"
                     /translation="MAITCDGPRLSVELNEEVVTRMDLDHWTAPNHRPDGSGHKFEIA
                     YNDHPRFGSVGLQDHGSECWYKNITLLPLKR"
     gene            complement(6746..>7131)
                     /locus_tag="VHS97_07045"
     CDS             complement(6746..>7131)
                     /locus_tag="VHS97_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3447993.1"
                     /translation="RTTIENGFVHGAPELVVEVSKATRYVDLGPKKADYEPAGVLEYF
                     VHAMDPDEIFWFGQDQGALVQRAAGDDGLYRSTAFPGLWLDPQALLTGHRKRMREVVD
                     LGCATPEHAAFVARLAAARTVGQAE"
BASE COUNT         1479 a         2192 c         2153 g         1307 t
ORIGIN      
        1 aagccgaagc cacgctcggt gctcgtcagc gcgaatcact cttcaagtct gcttcaaaat
       61 cgatcgcttg gccagcaacg agccggtcgc tctcgatcgt aagcttgggc gactcgccac
      121 cggcggcgat ctcgtcagtc cgcgattcac cgggaagcag ccccttgagc gtgcgagcgg
      181 atcgaacacg attcaccgtc aagaatgcat catggtaaat cggcgcgacc cccgtgtttg
      241 tgatcgtaat gcgagcgttg gaaggactcg cttcaaaggc cagaatgcgg aacttataac
      301 cgcacgccaa cccggccgtg cgcacgcggt ccaggggttg ataccgcggc tggtcattgc
      361 caatcatgaa cgtgagatgg aaatcggctg ccgctttttc gaatgagatg ccgtgtggcc
      421 cgctcgccgc caaggcctgc ttctggtcag cgcgggtgta atagctgaat tcgcctccgg
      481 ccggctggcg cttccagcgg tcgcgtccga cggcgttcca gtttggctca ttctcgcgcg
      541 cgtgctggcg gcagagaaaa gagtcgtcaa acacgccaaa cggcagcctc agaagctctc
      601 cgtccgctgc gaaaggggcc cgcgacggat cggctgcgtc aacggaaatc atccatggcg
      661 ttttttggaa ggcacgagcc agccggcgag caaactcggc ttgaaacggc ttatccggaa
      721 acgtcttgcc cagcttcatc ggtccatcat agatgtgata ctcggcccag aggccgaagc
      781 ccgtctcaag aaacgccaac cgaggatcct gatcgtagcg ttcagcgagc ttttcataaa
      841 acgcgagcgt gaatcgcttg agctccgggt gtgaccaatc cgcgaaggtg gtcggctttc
      901 cctcactctt gctgggcagc tcatgatagt cggagaggcc cttgatatag gctgggacgg
      961 tcgtctcgtg tccaggataa acgaagtaaa aacgaacgat cgcctgatgc ttgcgggacg
     1021 cgacgtcttc gagcagccgg tccatcacac gccaatcgta ttgatcgcgc cccgtgacaa
     1081 cgtcgccata cttcatgtag ctgtattcga gttgtacggc gtcggtgatt ctgtgctcgg
     1141 acgtggtcca gagcacaatc ccggtcatgg gctgcacgcg cgtgatctgg ctcttgagag
     1201 ccaacggtat gaagcgatcg tcggcgcggc attcgaccat cgcaccgatc agattggcaa
     1261 tcaccgcaag aatgatcgaa acagagctcc ggtctcgcgg acctggcaat ttcatcatct
     1321 gtttctctcc tgcgtgatat tgccagattt gcgcgggccc tctcaatccc catgcttgct
     1381 gcgcagccgc ggcgacagcc gcaagataag ctgagcccgc tcaaactcag ctaaccactc
     1441 ggcggtgttt acggcgaggg cctcgtcggc ggggggcacc agtcctgtcg ggcaggcggg
     1501 tcccagttgg tcggcggcca tgcggcgata gatcgacaga gtgcgctctc cataacggca
     1561 gacctcaaac caatcttcgc tcaaaaaggc gacgaccggc actcggtgcc cgccatttat
     1621 cgcgagcaga tcgcgcaact caggcttggc gtcacggtcg aggaaccgta gctcgattcg
     1681 agaggatgct tgcgcaaaat gatcaaaaac cgggcattgg ttgatgcagt cgccacacca
     1741 ggcccctgcc acacagagta ccggcattcg ccgcgcgaag ctgctgatca attcacgttg
     1801 cgcagacgtg agggcgaagc gtgcgtgcat cgcatcccac cgtgctcgtt gcgacggtgt
     1861 cgcatactga tcgagaaatt tatcgtaagg taatgcgctc tcaaagtggc tcgaccagtt
     1921 gaccataatt cttctcgtag acagtgaggt ccgcgtggtt ttgatgtcca acgaggctca
     1981 ttgtgtcaca cgcaagttcg tctggtcaaa tccagcccag ggtagccagt gcttgcaggt
     2041 gcttgcagtg tttgcgacgc gtattttcgg tctcggcgat ctggtaggtc caatcggcgc
     2101 agtcgcattg ggtagagccg ctggcaagcc ggcatgcgta gtagggacca ttttcgagcg
     2161 gcggattcaa gcgggtcagc cgccattgaa tcggcgccag ctcagttccc tggacgcgct
     2221 gagaacggta tgtctgccca gcgatcagga tcagtacgac aggtgccacg cccttcgccc
     2281 tttctcccag ggtgagcgtg gtcaacggcg gcagattgag tgccgcctcg agagccgggg
     2341 cgcctagctc ggcgccgagc cggtcccaag atcgctgagc ctcggccaag gcattgcgaa
     2401 agtgccaggt cgtgtggtgg aacgccccga actttgcttc cgcggcttca aggtcggcca
     2461 ccgacacgtc gagaatgagc cgaccgcgct gcaccagctc ggcggcgcag cggtcgggat
     2521 cggtctcatc gcgctcgcga atcggccgcc gaagaggtca gtgcggcgac ggaggggtct
     2581 gcctgcccga cgtcggccgt gatatctggg ccggctcggt gcggatcgag gctcgtactg
     2641 ctggtgctgt tgatgtctcg cggttgtttt gatttatcat tgcggcctcc atgcctattc
     2701 gagttggtgc gatcgcggta gagcgctaaa gtgtgtacca aatgtcagcg gcatgagcaa
     2761 gatggtcgag ccaggaaacg aatgccttgt gacgtgatgg tgcaacgtac cggcgttccg
     2821 tgtgacgacg gctccagtca ccgccgcgta agtcgattgc gcgcggtggc gtatgcatcg
     2881 agtattccgg atcgaccagg gtgaatggcc tccaggccga gcctcgattg gtcggccgac
     2941 gagaggcgtt tccccgtacc cgccagcacg accaagcgct gaccgtcggg taggttaaaa
     3001 agaccgtcga tggcattaag gtcgcgctga gcgacccgtg gcagcgccgt cctgaggata
     3061 aagcggagtc ggccgccggg caatggccga tcaccggccg gaccaccgcc gttggcggtc
     3121 cgctcgaaga ccgagggact gacaacggcg accacctgaa aagggttctc acgattgcgg
     3181 cgaagtatca tcgtgcgcaa cgcaaggagc gcatgggtct cactatggcg gaacaagccc
     3241 tcgagcagcc cgacgacgac ggtgacaacc acgatcgaca gcaaaaagac ggccaggatc
     3301 ccgcgctggc gatcatcgcg ccggcgtgcc cagcggtaga gcccgcggat taacaacacc
     3361 gacaccacaa ccaggtcgaa cgccagatgc aggcccagcg cggtcgcggt atcggcccgc
     3421 ccgtggatca cgtcttcgac ggccctgttg aggtcggcgc tggcccacca ggctatcgac
     3481 atggcagtcg cgggagccag caggatcagg gcgcgcaccg ataggcgtcg gttggcgagg
     3541 tcagcgattg tctgagcggc tagcaggctt accggcacca gcagcaacag gtcgaacgct
     3601 ctgcgcggcc cgcccggcca gatcgcaggg gccagtgcgg cgacagcaag ccagaccacc
     3661 caaaacgaac ccccgaccgt ttcccgcgtc tcggattcgt cgaccaatgc agaacggatg
     3721 gcgcgcacgg ccccaaacag agccagaggc agcgtcacag gcgccagctt gatcaatcgc
     3781 ggcagcagcc ccgtatggac atctcccgcc agactgtcgg ggggagcacg tagtgcaaac
     3841 aatgcctgcc acccgtatga tttgaccatc aagacatacc acggcaacgc caccgccagc
     3901 gcgattgaga gcgccaagag gccatcaact atgcccggac tgtcccgccg gccgcgccac
     3961 cagaaccgag gcagcgaatg ctgcgacgac gaagccattg atgggctcag atagaattgg
     4021 tgcaacaaga cgatcggaat cacgatcagg gccaatccgc ccagggaaag gagagctgca
     4081 cctagcgcga tgccgccgac cgcggcccag acgactggac cggaccatga ccagggtcgc
     4141 accgataggg cggccatccg ttcatgccaa ccgtaagcca agagtgcagc gagcaccccg
     4201 cagacgacca gcgtggccgg agtcgcttcc tgcatccgca acagcaagtt ttggttaaac
     4261 cccaccaaga tcgccgcagt cagccccaaa cccgccccgc gccagagccg gccgtgcaaa
     4321 tagaccaacc ccaccacgag cgcgcccgcg atgtaactgg gcaagacgct tgccaaggga
     4381 tcacggtcgt tgctcagcca gaacccaatt gcctctaacc aggcgtatag aggcggctgg
     4441 aacgccaccg agctaaacgc cgccgactcg gggacagact tgatctcggc cgccgccggc
     4501 atctgatcga gtaccaaacc gtcaagtacg gcgaggcccc gcaagcccca caaaggacca
     4561 ggcggagtaa ggtcccaggc attcaaggcc accatgcccg gcaacacggc gatcaacacg
     4621 accagcggaa agaccatcgc cgaccgtggt gctggacgga tcacaggtcg atccatcggc
     4681 ctgagcgctt cgcccgaacc gctcggcgct acagatcgac catgatccca ggcgaccggc
     4741 ggctcgcgcc gacgccgcag cgacagatcc ggctccatat gcccagatcc cccgcgaagg
     4801 ttttcctaca ccctgaaaat tcgcgctagg gcgcatccca cgccaaccat ggcgaacagt
     4861 accataatcc cgcgaatcgc gggatagcac atccgattca ggcgtcatga tgggctgact
     4921 catcgtcgcg gcgcgcctgg attcgttcga gattgcgtct caaagctccc aggccatcgt
     4981 cgccggtaat atattcgcgg taaggcatga cgagtcggag caaatcgggg tttgccgtac
     5041 ccacatcgtc gattacatgc cgcaaggccg cgagaaactg gtcaagccct tcgtccggga
     5101 gatggagccg agaaaccagc gacttcaggc cgttgagatt gggcggcgca gcgtgagaat
     5161 aagcaggggg ggcggcgctg agtgtggcgg tcgacgtcgg agcggccagg cgggaggcct
     5221 gggcgacacc tgacgtggac ggctggtcgt cgctggactc gcggatgcgg ggttgctcgg
     5281 catcgcgagc gcggcggccg gcctcgatcg cctgcaccaa ctgctcttgg gcctccgcca
     5341 cgtaggtagc ccaatcggtc gagaagtcct ggcggaacgc ctcgatataa cccggttcat
     5401 actgcctcga tagccacttg agttgatgga aaaccttgtg tgataatgcc tgcagctctt
     5461 cggtgacaag ggcgtcacgg tcctgcgagg cctggtgaaa ccggaagcgg ccgatccaga
     5521 ccttgagtcg agcatattgt tcgagtttgt tgagcccggc aaatgcacct agcccaacct
     5581 cgacctcgtg ggcgagaagt tggagttggc cgccggggaa gtgtgcctcg gcatcccaga
     5641 gcagcctatc gtcggcggcg aattgtgcgg ctaactcggg ctgtagccga tcaagctctc
     5701 gtcgtagctg ccggtaatgg tcggtcgccg tccgcagctc gcgccgcgcc tcctcgacat
     5761 cgtgaccgat ctgttcgaga tcggccttgg tctgacaccg gatgcgttcg atctcagcct
     5821 gcgacgtcgc gatcagccgc tcggcgtcgg cttccgatcc gagcgatgat ttgtacaggg
     5881 caagcttttt aaccaggtcc ttgagcaatt gctcatcggc attcgcctcc tccagggccc
     5941 gcaggctcgt gggtcgaagt gcgcggagct gctcgaccgc gcggcgagcg cgtagctcga
     6001 ccgcccgaaa cagttcagca gcgtgctgtg tctcaccttc gatcttacga gcacgagtct
     6061 tcgcttcggc ctggcggact aactccgcaa gccgctctaa cgctggcgtc atggtcgaca
     6121 ctctcgcact ttactcttga tcaaacaaac aaggatgcct ttaacgttta gatactctcg
     6181 accttcatta ctcacccttt atggcaattc ctggtacagc gccgcgcctg gcggctggga
     6241 tcatcgcctc cacagaaaat cacttggcaa ttcttcattg cgattgcgat ctcgcttgga
     6301 atgcatcata cacctccgcg gatcaaagtt gaattggggg gaaggaacgc ttcggcggac
     6361 tgaaatgagc tcgccctttg tccgtgccga ttgatccctc attcagcata ccggactgtg
     6421 catgtatgat gacagcgcca tcgccaaggt ccatacattc gttgaatcaa ggaatgggaa
     6481 tggcaatcac gtgcgacggt ccgcggcttt ccgtcgagct caatgaggag gttgtcacgc
     6541 ggatggacct ggaccattgg acggccccca accaccgacc cgacggttcc gggcacaagt
     6601 tcgaaatcgc ctataatgat catccccggt tcggctctgt cggcctgcag gatcacggat
     6661 cggaatgctg gtacaagaac atcacgctat tgccgctgaa gcggtgaacg ccggtgcggc
     6721 gagagagaat cgatttgcga caagattact cagcttgacc aacggttcgg gccgcggcta
     6781 agcgcgcaac gaacgcagca tgctcaggcg tggcacaccc gaggtcgaca acttcgcgca
     6841 tccgctttcg atgccctgtg agaagcgctt gcggatcgag ccacaggccc ggaaatgcag
     6901 tcgaacggta caacccatcg tcgccggccg cccgttgcac cagcgctccc tggtcctggc
     6961 cgaaccagaa gatttcgtcc gggtccattg cgtgtacgaa gtattcgaga acccctgccg
     7021 gctcatagtc agcctttttt ggtccgaggt cgacatatcg agtcgccttg gaaacctcga
     7081 cgacgagctc cggagcacca tgaacaaatc cgttttcaat cgtcgttcga c
//
LOCUS       DATAQL010000408         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2482489, whole genome shotgun sequence.
ACCESSION   DATAQL010000408 DATAQL010000000
VERSION     DATAQL010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482489"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VHS97_07050"
     CDS             complement(<1..228)
                     /locus_tag="VHS97_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447994.1"
                     /translation="MIARLYFCAALVLGQAAGGLHGGEPPAQSVSPTAVVRLFNGHDF
                     AGLSTWLEDTKHDDPRRVFRVEDGLLHITGDG"
     gene            complement(323..1231)
                     /locus_tag="VHS97_07055"
     CDS             complement(323..1231)
                     /locus_tag="VHS97_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3447995.1"
                     /translation="MTDRPLRILVIGAHPDDADLKAGGTSAKWCALGHVVRLVSLTNG
                     QAGHHTMYGPQLARRRRAEAKAAGSAIGASYEVFDQPDGELDDRLELRHQVIRLIRSF
                     HPDLVITHRTTDYHPDHRFTGLLVQDASYLLTVPAICPDVPHLPFCPVILSFSDAFTK
                     PCPFEPHVVVDIDDEFDRVVAMIHCHKSQFYEWLPYNAGFVEEVPREDSAREEWLAER
                     LRSRIRPLADRYRDLVIRTYGQERGERVRLIEAFEVSEHGAPLNAVARARLFPFLPAV
                     GSDCSPFTRKIWVDIPDGDERHASAV"
     gene            complement(1325..3238)
                     /locus_tag="VHS97_07060"
     CDS             complement(1325..3238)
                     /locus_tag="VHS97_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3447996.1"
                     /translation="MLVLSMWCGLAAGELEIGARVVPRALSATPQLNMMTRHFVWLVP
                     LVNLVLFLGLGLVLVLAMRFWPRRARWWTPRLIVAAAILPMLVLAGRRIYLEAWLLVA
                     LGVAFQAAPALERQPAERRRRLAWTLPLLLGLVLLQAGWIYGGDQIKQWREDGRPLPP
                     AGSPNVLLIVLDTVRADHLSLYGYPRPTSPNLEQLARRGILFDQARAPAPWTLASHGS
                     FFTGRWPHELGVQWLAPLQGNFTTLAEYLGAHGYATAGFVSNAGYCSYETGLSRGFTH
                     FEDYVLARLGTLRAAGLLDVAVKTFGHLTLPLDYGWFHRVRAIVSDWFFAPERKTAAR
                     INRDFLHWLAPRRESARPFFVFLNYLDTHTPYLLPPGARHRFGVNPVTKKDYVEVLME
                     WSSLKKQRLPQRYSSLAHDAYDSCVAYLDDQLGALFDELQGRAILDRTLVIITSDHGE
                     GLGEHGLFMHGESLYRTEIRVPLLILLPTHRPSGSLVRETVSLRDLPATIVDLIGLGK
                     GSPFPGRSLARMWADLPTGAAPVETEVVLSELASPNPSDPNQGRSPVYRGPLISLALG
                     DFVYIRNEGDGSEELFNQREDPDELSNLAHVDTMLPILRRFRDHLPQAKARNPALADS
                     GASPRVASTPKTP"
     gene            complement(3318..>3485)
                     /locus_tag="VHS97_07065"
     CDS             complement(3318..>3485)
                     /locus_tag="VHS97_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEX3447997.1"
                     /translation="ISEGQFENANSLHPGGCNVLFADGSVHFVKSSISINTWWSLGTR
                     ANGEVISSDSY"
BASE COUNT          741 a         1040 c         1108 g          596 t
ORIGIN      
        1 gccgtcgcca gtgatgtgga gcaggccgtc ctcgactcga aagacccggc gtgggtcgtc
       61 gtgcttcgtg tcctcgagcc aggtggagag gccggcaaag tcgtgaccgt taaagaggcg
      121 gacaactgct gtcgggctca ctgactgggc cggcggctcg ccaccgtgaa ggccgccggc
      181 cgcctgcccc aatacgagcg cagcgcaaaa atacaatcgg gcgatcatgc ggcctccttc
      241 cccgacggcg ggcacgagca gccattttac ccgcgaaaaa ctcacgcctg ctaggcagcg
      301 atgaaagccg ttgtgactcg gattatactg ccgaagcatg gcgctcgtct ccgtcgggga
      361 tgtcgaccca aatcttccgg gtgaacggcg agcaatctga cccaacggcc ggcagaaacg
      421 ggaacagccg tgcccgggct acagcattca gcggcgcccc gtgttccgag acctcgaacg
      481 cctcgatcaa gcgaacgcgc tcgccccgct cttgaccgta cgtccggatg accaggtcgc
      541 ggtaccggtc agcaaggggc cggatgcgcg agcgcaaacg ctcggccagc cactcttctc
      601 gagccgaatc ctcccgcggc acctcctcga cgaaaccggc gttgtacggc agccattcgt
      661 agaactggga cttgtggcag tggatcatgg ccacgacgcg atcgaattcg tcatcgatgt
      721 ccacgacgac gtgcggctcg aacgggcatg gcttcgtaaa ggcgtccgag aatgagagga
      781 tcacgggaca aaaagggagg tgcgggacgt cgggacagat ggcgggcacg gtgagcaagt
      841 aggatgcatc ctggaccagc aagccggtga atcgatgatc ggggtggtaa tccgtcgtcc
      901 ggtgtgtgat gacaaggtcc gggtggaatg atcggatgag gcgtatcacc tgatggcgca
      961 gttccaagcg gtcgtcgagc tcgccgtcgg gctgatcgaa aacctcataa cttgcgccga
     1021 tggcggaccc ggccgctttg gcctcggcgc gccgccgacg tgccagctgc gggccataca
     1081 ttgtgtggtg accggcctgg ccattggtca gactcaccag gcgcacgaca tgtcccagag
     1141 cacaccactt ggccgatgtc cctccggctt tgaggtcggc atcgtccgga tgcgcaccga
     1201 taactagtat tcgcagaggt cgatctgtca tgaaagctgc tcctcgattg cacggcccaa
     1261 gcgcccgatc cagggtagcc cgccgcgggg gccatatttt tgtcggccgc gatatctgaa
     1321 taaattaggg agttttcggc gtggaggcga ctctgggcga agcgccggaa tccgcgaggg
     1381 ccgggttacg cgccttcgcc tgcggaaggt ggtcgcgaaa ccgccgcagg attggaagca
     1441 tggtgtcgac gtgggcgaga ttgctgagtt cgtcggggtc ctcacgctgg ttgaataatt
     1501 cctcgctccc gtcgccttca ttgcggatgt agacgaaatc ccccagggct aaggagatga
     1561 gcgggccgcg atagaccggg gatcgtccct ggttcggatc ggacggattg ggcgacgcga
     1621 gctcggaaag cacgacctcg gtttcgacag gagcggcccc ggtcgggaga tcagcccaca
     1681 tccgggccag cgaccggccc gggaacggcg atcctttacc caagccgatc aggtcgacga
     1741 tggtcgccgg caggtcgcgc aggctgacgg tctcgcgcac gagcgatccg gacgggcgat
     1801 gcgtcggcag caagatcagg aggggtactc ggatttccgt gcggtagaga ctctcgccat
     1861 gcatgaacag tccatgctcg cccagcccct cgccatggtc ggatgtaatg atgaccagcg
     1921 ttctatcgag gatggcacga ccttgaagtt cgtcaaataa cgcaccgagt tgatcatcca
     1981 ggtatgccac gcaactgtca taagcgtcgt gagccaggct cgagtatcgc tgaggcagcc
     2041 tctgcttttt gagggagctc cattccatca ggacttccac gtagtctttc ttggtcaccg
     2101 ggttcacgcc gaaccggtgc cgggcccctg gcggcagcaa gtacggggta tgcgtatcga
     2161 gataattgag aaacacgaaa aagggacgcg ccgactcgcg acgcggggcg agccagtgga
     2221 gaaagtcgcg attgatcctg gccgcagttt tacgctcagg agcgaagaac caatcactca
     2281 caatcgctcg aacccgatgg aaccaaccgt agtccagagg gagggtcaag tggccgaacg
     2341 tcttcacggc cacatcgagc agacccgctg ccctgagggt accgagtcgc gcgaggacgt
     2401 aatcttcaaa gtgagtaaac ccacggctca ggccggtttc ataagagcaa tagccggcat
     2461 tggagacgaa accggcggtc gcatagccgt gggcccccaa gtattcagca agcgtcgtga
     2521 aattcccctg caacggcgcc agccattgca caccgagctc atggggccag cggccggtga
     2581 agaaactgcc gtgcgaggca agggtccatg gcgcgggcgc gcgcgcctga tcaaaaagaa
     2641 tgccacggcg ggccagttgc tccagattgg gagaagtcgg acgtggatag ccgtagaggc
     2701 tcaaatggtc cgcacgcacg gtgtcgagca caatcaagag cacgttaggt gagccagccg
     2761 gtggcaaagg acggccgtct tcgcgccact gcttgatctg atcgccgccg tagatccagc
     2821 cggcctggag caggaccagc cccaacagga ggggcaaggt ccaagccaac ctgcgccgcc
     2881 tttcggccgg ctgccgttcc agtgccgggg cggcctgaaa cgccactccc agcgcgacca
     2941 gtaaccatgc ttccaggtaa attcttcgtc cggccagtac cagcatcggc aggatggccg
     3001 cggccacgat cagccgcgga gtccaccatc tcgcccgacg gggccagaat cgcatcgcca
     3061 gaacaagaac caagccaagc ccgaggaaca gcacgagatt gacgagtgga accagccaga
     3121 cgaagtgccg ggtcatcatg ttgagctgag gcgtcgcgct caatgctcga ggaacgaccc
     3181 tggcgcctat ctcgagctcg cccgcggcca gtccgcacca catggaaagg accagcaatt
     3241 cgaccgatcc aagatcacgt actggttcgg gcgatcggga ccagctttca gaatcgaaga
     3301 cggtcatcta ttgttgttca atagctatcc gaggagatga cttcgccatt ggccctggtt
     3361 cccagggacc accaggtatt gatggagatc gaactcttga cgaagtgaac actgccgtcg
     3421 gcgaacagca cgttgcagcc gccaggatgg agactgttgg cattttcaaa ctggccttcg
     3481 gaaat
//
LOCUS       DATAQL010000409         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2788815, whole genome shotgun sequence.
ACCESSION   DATAQL010000409 DATAQL010000000
VERSION     DATAQL010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788815"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(862..1401)
                     /locus_tag="VHS97_07070"
     CDS             complement(862..1401)
                     /locus_tag="VHS97_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX3447998.1"
                     /translation="MTTIRYEWNDGDTRRILELVHVEGTNGRPYGFGEGDDARRIEVR
                     GFFAATVPVTQALWTHVMGADNPAVRRGAQLPLENVSWDEIVRPSGFLDRLNVSLIRS
                     QVTAQIPPGAWVFRLPSETEWEYAARGGPHWRDGLRFSGSNDIDRVAWYDRRGGDHTQ
                     EVAQKGPNQLGLYDMSGNV"
     gene            complement(1653..2081)
                     /locus_tag="VHS97_07075"
     CDS             complement(1653..2081)
                     /locus_tag="VHS97_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3447999.1"
                     /translation="MKMQRLAVALTLVNLGILAFLLLSHVRPAVATATADDPAPMLRG
                     RGLQIVDERGKVRASIQVLPASVFKQTGEKIPETVLLRLIDPNGRPGVKIGTSADGGG
                     MSLAGDSEKREWNGAQILANAEGTSVILTNKDGKKQVLKP"
     gene            2246..2374
                     /locus_tag="VHS97_07080"
     CDS             2246..2374
                     /locus_tag="VHS97_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448000.1"
                     /translation="MALPFRILVKVFHAASSGYTSRFLGLYSRFIARTVFARDFEA"
     gene            complement(3044..>3407)
                     /locus_tag="VHS97_07085"
     CDS             complement(3044..>3407)
                     /locus_tag="VHS97_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448001.1"
                     /translation="KNLINAIARFLLGLKRPSKAANSRLLRERGETLSSFIIREATSE
                     DIPALAALHVKAWNETYWNVKHPPSYGLREHQWRDQFQKTDGNWFCFLVEDPGGALVG
                     FRRGCLSPPATCPVIPAN"
BASE COUNT          679 a         1027 c         1021 g          680 t
ORIGIN      
        1 aagctggagg tcgtcaccct cgccatatgt tcggagcatg tagccggatg gcaagtgtgg
       61 ctcaaggaca tcgaggttaa gtcgcggcca caggagatag agacacggag gtcgagcttc
      121 acccatcgac tagtctccga atgtgtgtca tgccacgcag ccaggatcca agctgagcgg
      181 actggcccgc caaggatctc ctatgatgct agtcgaaccc atgcgtggaa agaacgccgc
      241 ggccgacgga atgaagatcg accgccgcgg ctgacggcgt taagaacgcg agagcagtcc
      301 tacgaaacgc ttcgctgcat cgcatcgtcc cggccaagtg ccatccgccg gactccgttg
      361 gccgggattg ctttatcgtg gcgcgcccga gagcgatacg caagggaacg cgtaaacgtt
      421 cacgagtcga tggtgacaag agtatagcag cgtatggaga agcgaagata ccgccggggt
      481 gcttcggtcg atccgaccga cgcgcgcaag gcgaccattc gacgcttcgg tcgatccggc
      541 cgaagcgcgc aactaaagtc tctgcgatta attcagggac ctaccgctcg ggagcgactg
      601 acgaggccgc ggtgggctgc agcggttagt ttggccccgc gctcaagcac cctctcatgc
      661 gcctgccagc gcaagccgga atccaatgca cccgtcgtga tacgtgcgtt cgatctgata
      721 gcgcttcgag acggtacagt ggatcgccca gttgtgaaag cacccaccgc gaaggacgcg
      781 ctcatcactt tcgccggtag ccgcactgcc gtcgacgggg atggcgctga gatcccccgt
      841 aaaggagtcc tggcaccact cctagacgtt gccggacatg tcgtagaggc cgagctggtt
      901 cggacccttc tgtgccacct cttgggtgtg atcaccgccc ctccggtcgt accaagccac
      961 gcgatcaatg tcgttgctgc cgctgaagcg aagcccgtca cgccagtgtg ggccgccacg
     1021 cgccgcatat tcccactcgg tctcggaagg cagccgaaag acccaggcgc ccggaggaat
     1081 ttgggccgta acttggctgc gaataaggct cacgttgagc cgatcgagaa acccggacgg
     1141 ccgcacgatc tcatcccatg acacattctc cagggggagc tgtgccccgc ggcggaccgc
     1201 gggattgtct gcacccatga cgtgggtcca cagcgcctga gtcactggga ccgtagccgc
     1261 aaagaaacct cggacctcga ttcggcgcgc gtcgtcgccc tcgccgaaac cgtacgggcg
     1321 gccgttcgtg ccttcgacgt ggaccagctc gagtatccgg cgtgtgtcgc cgtcgttcca
     1381 ttcgtagcgg atcgtggtca tgttcggctc cgtcgcgcca ttcgtgaccg tgcgcggtcg
     1441 aacgtttgtt caggaatctc ctcgccgaac gaggaagcca agcatccggg gcggcttgag
     1501 tgccggtcta cgcgaaaaga ggtaaacgca ttttaatgct atgtatgttt tttgccagaa
     1561 tctttaccga cgtccagatg gacacgagaa cctcgcggaa gaaggttaac cgactccgcc
     1621 aattgccgtc tttcgctccg cgaaagccgc cattagggct taagcacctg ctttttgccg
     1681 tccttattgg tcaatatcac ggaagtgccc tcggcgtttg caaggatttg ggcgccgttc
     1741 cattcacgtt tctcggagtc gccggcaagg ctcatgcctc caccgtcggc ggacgtgccg
     1801 attttaacgc cgggccggcc attcgggtca atgagtcgca gcaatacggt ttcagggatt
     1861 ttttcgccgg tttgcttgaa gacggacgcg gggaggactt gaatgctggc gcgaaccttg
     1921 ccgcgctcat ccacgatctg gagcccgcgg cctcgaagca ttggcgcagg atcatctgcc
     1981 gtcgctgtcg cgacggcggg gcggacatgc gacagcaata ggaacgccag gattccaaga
     2041 ttgaccagcg tgagggcaac ggcgagtcgc tgcattttca ttgggttctc ctcgatgtgt
     2101 gtgcttccag ccgagccgat aggcacggtg atgcttagtg gtagcgactt gcaaacataa
     2161 agggacgtgc tcgcaaactg ggacgttcgc gtgctcaaat tagccgaggc gcagcggtgc
     2221 tccgtcaaac gaggcgaact tcctcatggc tctccccttt cggatcttgg tgaaggtctt
     2281 tcacgcggcg agttcgggct acacctcgcg atttcttggc ctctactccc ggttcatcgc
     2341 ccggacggtt ttcgcgcgcg atttcgaggc gtaagtattc ggatactctt ggcgaattcg
     2401 gccaggccgt ctgggacggc gattccacct tccctggggg cccatcttgc ccaagctccg
     2461 tcccgtcggt cgcccgcggg aattcgccaa cagtatccac cacggctggt aaagtcgcca
     2521 cactcgcttc gcctttccca cggatttcgt gagcgcccac gactgatccc gttcgatccc
     2581 ttactcgtgg atctgacgac cagttgcggg gattggcctc gcgatccgct cgggtattga
     2641 ccaacgacgt taaggccgac gagatgccga cgactctcag aacggcggcg gagagctacc
     2701 tgcgtgccaa agccctttcc cgcgggacgc gcaacgagta cctctcgacc ctcaggaagt
     2761 gggagatgtg agccccaggt ttcagtgatt caagcgcatt caacggggca aatagatgca
     2821 agagttttca agtcccgcca gccgtagccg ccgtgaaact cgcctttctt gctgagcagc
     2881 ctttcccctc ccaaggcttc gtggaaccgg caggagggat tctgtgcctc gccaaacagt
     2941 accatcgagg aaataccctg gctcaaaaaa cggcgggcga cgtgaccgac cagccgacgc
     3001 ccaaggccca ggcgttggca atcccggagc aggtagatct tgttcagttc gccggaataa
     3061 ccgggcaggt cgctggaggc gaaaggcaac cccttcgaaa acccactaaa gcgccgccag
     3121 ggtcttcgac caggaaacaa aaccagtttc catctgtttt ttggaactga tcgcgccatt
     3181 ggtgttcacg caagccgtag ctcggcggat gcttgacgtt ccaataagtc tcgttccagg
     3241 ctttcacgtg cagcgcagcc agcgcgggga tatcttcaga ggtcgcttcc cggatgatga
     3301 aagacgagag cgtttctccc cgctcccgaa ggaggcggga attggcggcc ttcgagggtc
     3361 gtttgagacc caacaggaaa cgtgcgatcg cgttgatgag gttcttc
//
LOCUS       DATAQL010000410         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4468626, whole genome shotgun sequence.
ACCESSION   DATAQL010000410 DATAQL010000000
VERSION     DATAQL010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4468626"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VHS97_07090"
     CDS             <1..1125
                     /locus_tag="VHS97_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3448002.1"
                     /translation="LPTNLFEAFDSTGGVGTRGFNIISHRLEELLHVDLTKVVVADPI
                     ARHRSVSRITLRQVLLDGLDDVVHFDKRFQRYEESRDGQVVAHFDDGSSTSGDILVGA
                     DGGNSKVRAQFLPHAERIETGIVGIAGKVPLNEETRAWLPRPILDGMATVSAPKGRFM
                     FCASMIHGKDGHAAAKIGGADAAEELHPGLLFDNTSDYIFWAFSAHRRSYPHDADLNE
                     HNGEHLRQIVLGMITEWHPQFHRLVRAADVSTLAVLPIRTSVPVAAWPTRNVTLLGDA
                     IHSMTPYRGIGANVALRDAALLCRNLAAANRGETTILEAIHAFESEMIVYGFDAVRTS
                     RKAMEQSHADSRIGSAIAKVVFKTLNAIPPLKRRVFLGSD"
     gene            complement(1148..1324)
                     /locus_tag="VHS97_07095"
     CDS             complement(1148..1324)
                     /locus_tag="VHS97_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448003.1"
                     /translation="MNPKSARVLFGAALALYILWFVALVTLAVISGERPVTGKTKPAP
                     APATSLSVPERTSE"
     gene            complement(1389..1853)
                     /locus_tag="VHS97_07100"
     CDS             complement(1389..1853)
                     /locus_tag="VHS97_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HEX3448004.1"
                     /translation="MNFRDELARIIAGDPRYSIEVYAFVLEALNHAKYVKLKRKARDR
                     DKSRSGEPDRQRRPSSSQSKKAPVSGHVTGRELCDSVRRLALRQFGSMAATVLSHWGV
                     RSTSDIGDVVYNMIAAGDLEKTPGDSRSDFDEVFDFAKALKPKFALPAEELA"
     gene            complement(1880..>2729)
                     /gene="ricT"
                     /locus_tag="VHS97_07105"
     CDS             complement(1880..>2729)
                     /gene="ricT"
                     /locus_tag="VHS97_07105"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX3448005.1"
                     /translation="IPGRIQGPAGLRTCAGPAGNGPSDRGIELGDVLCPVSEKTSRFL
                     TNPAQGEILRAATPEDQEQEARLAEDRNPVFATCHELIAKRRLQMNLVDVELIHGRER
                     VVFYYLAEKRVDFRELVKDLARVLRTRIEMRQIGVRDEAKLLADYGDCGKPVCCNTHL
                     TRMPPVSMKMAKIQKTTLDPAKISGRCGRLKCCLRYEYDTYRTMERTLPPVGSRVVTP
                     KGQGRVVAQDILVQKLVVEFEDHRRIIVGRDDILSAEPPKGRIVAQSDLDDTDQDDSG
                     FESSWT"
BASE COUNT          526 a          839 c          807 g          557 t
ORIGIN      
        1 ctgccaacca atcttttcga ggctttcgac tcaaccggtg gcgtgggaac ccgaggattc
       61 aacatcatta gccaccggtt ggaggagctc ttgcacgtcg atctgaccaa ggtcgtcgtt
      121 gccgacccga tcgcgcgcca ccgatcggta agccgcatca cgttgcggca ggtcctgctt
      181 gatgggctcg acgacgttgt tcatttcgac aagagattcc agcgatacga agagtcccgc
      241 gacggccagg tcgtcgccca cttcgacgac ggcagctcaa ccagcggtga tatcctggtc
      301 ggtgccgacg gcggcaattc caaggtccgc gcccagttcc tcccgcatgc cgaacgcatt
      361 gagactggga tagtcggtat cgcgggcaag gtaccgttga atgaggaaac ccgagcctgg
      421 ctaccgaggc cgatccttga tgggatggct acggtgtcgg cgccgaaagg ccgcttcatg
      481 ttctgcgcct ccatgatcca tggtaaggac ggtcacgccg cggccaaaat cggcggtgcc
      541 gacgcggccg aggaacttca tcccggattg cttttcgaca acaccagtga ctacatcttc
      601 tgggcgtttt cggctcaccg gcggtcttat ccgcacgacg ccgatctaaa tgagcacaac
      661 ggcgagcacc ttcgccagat cgtcctgggg atgatcactg aatggcatcc tcagttccat
      721 cgactggtcc gcgcggccga tgtttcgacc ctcgcggtct tgcccattcg gacgtcggtc
      781 cccgttgcgg cctggccgac ccgaaacgtc acactgctag gcgacgccat tcacagcatg
      841 accccctatc gtggcatcgg tgccaacgtc gccctgcgcg acgccgcctt gctttgtcga
      901 aacctggcgg ccgccaaccg tggcgagacg accatcctgg aggcgattca tgcctttgaa
      961 tccgagatga ttgtgtacgg gttcgacgcc gtacgcacgt cgcgaaaggc catggagcaa
     1021 tctcacgcgg acagccgtat aggttcggcg atcgcgaagg tcgtcttcaa gacgctcaac
     1081 gcgattcctc cactcaagcg ccgggtgttc ctgggaagcg attagcttga cagatgtcac
     1141 tgagaaatta ttcgctcgtt cgttcgggga ccgagagaga agtcgcaggc gcgggtgcgg
     1201 gcttggtctt gccagtgact ggccgttccc ctgatatgac agccaacgtg accagggcca
     1261 cgaaccacag tatgtacagc gccagtgccg cgccgaagag cacgcgagcg gacttcggat
     1321 tcacaaccaa tctcctttcg ctcagattgg atctcgatca accggcgaat ccgtggactt
     1381 tggcgtcgtc aagcgagttc ctcggctggc agtgcaaact tgggcttgag cgccttggcg
     1441 aagtcgaaga cctcgtcgaa gtcagagcgc gagtcgccgg gcgttttctc gagatcgccg
     1501 gcggcgatca tgttgtagac gacgtcgccg atgtcggaag tcgaccgtac gccccagtgg
     1561 ctgagcacgg tggccgccat cgaaccaaac tgccgaagag ccaatcgacg aactgaatcg
     1621 cacagctcgc gtccggtgac gtgacccgaa accggcgctt tcttggattg ggaggacgat
     1681 ggacggcgct ggcgatcggg ctctcccgag cgagacttgt cgcgatcgcg ggcttttcgc
     1741 ttcaatttca cgtacttggc atgattgaga gcctcgagga caaacgcgta tacttcgatc
     1801 gagtagcgag ggtcgcccgc aatgatccgg gccagctcgt cgcgaaaatt catgggttcg
     1861 tccctccttg cagcggcggt catgtccagc tcgattcgaa cccggagtcg tcctggtcgg
     1921 tatcgtcgag gtcagactgg gcgacgattc gtcccttcgg cggctcggcg ctcaagatat
     1981 cgtcccggcc gacaatgatc cgtcgatgat cctcgaactc cacgaccagc ttttgaacca
     2041 agatatcctg ggcgacaacg cggccttggc ctttgggagt gacaacccgc gatccgaccg
     2101 gcggcaaggt ccgctccatc gtgcgatagg tgtcatattc gtatcgcaga cagcacttga
     2161 gacggccgca tcggcccgaa atcttggccg ggtcgagcgt ggtcttctgg atcttggcca
     2221 tcttcatcga gaccggtggc atgcgcgtca ggtgcgtgtt gcaacagacc ggtttgccgc
     2281 aatcgccata atcggccaac agctttgctt cgtcgcgcac tccgatctgc cgcatctcga
     2341 tccgtgtccg cagcacgcgg gccaagtcct tgaccaactc acggaaatcg acgcgctttt
     2401 cggccagata ataaaagacc acgcgctcgc gaccatggat cagctcgaca tcgaccaagt
     2461 tcatttgcag tcgtcgcttg gcgatcagct catggcaggt agcaaagacg gggttgcggt
     2521 cctcggcgag ccgagcttcc tgctcttggt cctcgggtgt cgccgcacgg agaatttcac
     2581 cctgagctgg gttggtcaga aaccgggatg tcttttccga aacggggcag agcacgtcac
     2641 ccagctctat gccccggtcg ctcggaccat tacctgctgg ccccgcgcat gttcgcaatc
     2701 cggcaggccc ttgtattcgc ccaggaatc
//
LOCUS       DATAQL010000411         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_803123, whole genome shotgun sequence.
ACCESSION   DATAQL010000411 DATAQL010000000
VERSION     DATAQL010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803123"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VHS97_07110"
     CDS             complement(<1..539)
                     /locus_tag="VHS97_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3448006.1"
                     /translation="MNGNHNDVVNPTPVIEYHSDEELMRQLAAGQPEALGALHARYAS
                     LIYGVAARSLDRAAAEEISQEVFLAVWRHAASFDPTRGTFRTWVLQITRSSLLNELRR
                     RGRRPRAASQSNNSSSEHLPDPGLDPAESVWREHRRAVVREAVQALPPPQREALSLAF
                     LEDLTNEQVADFLKLPLGTT"
     gene            complement(547..1644)
                     /locus_tag="VHS97_07115"
     CDS             complement(547..1644)
                     /locus_tag="VHS97_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3448007.1"
                     /translation="MTSNLAMIERRPHAARLGHQVSAAMVACVVTKSERDRRSRLGVA
                     PVPDAELGFAESSGGRHVVEKRARRMLEARIRFVGHPDFDDSTATAEILGPMPGRVGG
                     GTSPGKGRVRDILPLSDADFQDSKFLTREQEVHAFRQMNFLKYQAAQLKEAINPSQAR
                     SADLDRIEELLRQAGAIRNRIIRSYVGLVISIVRKCAGPCQDFLDLVAEGNMSLIKAS
                     ERFDFARGARFSTYATWAIINNFAGRSRRERSRRVRFATGREGLLQSLADHRESGRAD
                     ALDQQPSREIVSSMLDCLNDREQTIVVRRFGLAGDKQTLAQVGRSLGISKERVRQLEF
                     RAIKKLRTLADAQKFGPIDESASGGDRGQDG"
BASE COUNT          329 a          559 c          593 g          343 t
ORIGIN      
        1 gtcgtgccca gcggcagttt gaggaagtcg gcgacttgct cgtttgttag gtcttcgaga
       61 aaggccagag agagcgcctc acgctgggga gggggcaagg cctgcacggc ctcccgcacg
      121 acggcgcggc gatgctcgcg ccagaccgac tccgcggggt cgagaccagg gtcgggaaga
      181 tgttcgctac tggaattatt ggactgggag gccgcgcggg gcctccggcc gcggcggcgc
      241 aattcgttga gcaggctcga gcgggtgatc tgcaataccc aggtgcggaa cgtaccacgg
      301 gtcgggtcaa agctggcggc atgccgccag acggcaagga agacctcctg gctgatctcc
      361 tcggcggcag ctcgatcgag agacctggcc gccacgccgt agatcaacga ggcatagcgc
      421 gcgtgaagtg caccgagcgc ctccggctgc cctgcggcga gctggcgcat cagttcctcg
      481 tcgctgtggt actcgatcac aggcgtcggg ttcaccacgt cgttgtggtt cccgttcatc
      541 gacgattcat ccatcctgcc cacggtcgcc tccagatgct gattcgtcaa ttgggccaaa
      601 tttctgggcg tcggcgaggg tacggagctt tttgatggcg cggaactcga gttggcggac
      661 gcgttccttg ctgataccca gcgatcggcc gacctgagcg agggtctgct tgtcgccggc
      721 gagtccgaag cggcggacga cgatcgtctg ttcccgatcg ttgaggcagt cgagcatgct
      781 tgagacgatt tcccgagacg gctgttgatc cagtgcgtcg gcgcggccac tttcacggtg
      841 atcggcgagg gactgcaaca gcccctcgcg gcctgtggcg aatctgacgc ggcggctccg
      901 ctctctgcga cttcgcccgg caaagttgtt aatgatcgcc caggtcgcat acgtactgaa
      961 ccgggcaccg cgtgcgaaat cgaatcgctc cgacgccttg atcaacgaca tgttaccctc
     1021 ggcgaccaag tccaagaaat cctggcaggg cccagcgcac ttcctgacga tggagatgac
     1081 cagcccaacg tatgatcgaa tgatccgatt tcggatcgcg ccggcttgcc gaagtaattc
     1141 ctcgatgcgg tcgaggtcgg cagagcgggc ctggctgggg ttgatcgcct ccttgagctg
     1201 ggcggcctga tacttgagga aattcatctg gcggaacgcg tgaacctcct gctcgcgcgt
     1261 taggaacttg gagtcctgga aatcggcgtc cgataatggt agaatgtctc ggacccggcc
     1321 cttacctggg cttgtgccac ctccgacacg cccgggcatc gggccgagga tttcggccgt
     1381 agcggtcgag tcgtcgaaat cggggtggcc gacgaacctg attctcgctt ccaacattcg
     1441 acgcgcacgc ttctcaacga cgtgccggcc gccactcgac tcggcgaagc cgagctcggc
     1501 atccggcacc ggcgcgaccc caagacggga tcggcggtct cgctcgctct tggtcaccac
     1561 gcaagctacc atcgcggcag agacttgatg ccctaaccgc gccgcatgcg gccttcgctc
     1621 aatcatcgcc agattggaag tcatggatca ggtccttatg ggccgcggag cgctagccct
     1681 ctcgactctt gttgttggat gccggctgcg ctggaaccac ataggatcga gcggtctggc
     1741 aaaaggaggc cccggatccg tttacactcg agatccgccc agagcacaaa cggctccgag
     1801 atcgaggatg ggaggacggc acgg
//
LOCUS       DATAQL010000412         6736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3553300, whole genome shotgun sequence.
ACCESSION   DATAQL010000412 DATAQL010000000
VERSION     DATAQL010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3553300"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2945)
                     /locus_tag="VHS97_07120"
     CDS             complement(<1..2945)
                     /locus_tag="VHS97_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3448008.1"
                     /translation="MQDEQRRRWARGERVLVETFLPTLTETIRGEDDVLDLIYYEVII
                     REELGEKPQLEEYLHRFPLFARQLRDQFEVHHGLNLTDLLGSARHSDASWRSSVQPES
                     WVATGTGPSIRGHEVIRELGRGGMGVVYLAWQTGLNRLVAVKMILAGEYSQPRDRARF
                     RGEAAAVARLEHPNLVKVYEIGELDGYPYFTMEYVDGGRLAEVLRGTPLASRRAAALV
                     ETLARATHAAHDRGVIHRDLTPSNVLFSRRGEPKIVDFGLAKLTVGGLGRTVTGEIIG
                     SPSYMAPEQATGQSKEVGPAADVYSLGAILYDMLTGRPPFKGESVLDTLAQVVHEEPV
                     SPCRFQPKVPRDLETVCLKCLSKEPSRRYSSAADLADDLRRFLDGEPVHARPIGAISR
                     AIRWARRRPRLAALLAVYSLGAIVAVAAIAGKAQEARQALRRAERDQKAEKAQRIAAE
                     SARESESQQRRLYQGISSVLLRDRALRHCDDGDVGRGLLWLAQSLKLVPDDDDNLKRA
                     IRTNLAGWQGRLHPLQSVLDHSSRVLAAVWSADGRFVLTTDAEKSARVWDVAGGDGPA
                     RTIRLPRSATVAAFSPDGAMVLTAAGQEIRLWATASGKPVFEKGLDLGKGAELLSYVF
                     SRDGHRLWTTSRRGQTAWLQSWLIDTGQRAGAEIELGRGITLATFSPDGEALVTASEV
                     REVGARLWRTADGRIVRALKEHTSRVAAVAFSPNDGHSFVTGSYDRTCRLWDSKSGAP
                     LGPVFRNSGAIRSVAFNPDGKTILIGGQDRLARLWDLSDNATSRAIMRHPDAVKTVGF
                     SSNGLVTLTVSSDQVRLWDAETGEMLGAPLPHVKEVLDASFSPNGKAVLTRGRDSAIR
                     IWSTAPIRSGERRLRHKGWVTAVAFHPQDGESFLTGVGGSAAKVLSWSTTTDRQPDVT
                     LDHLGPVLSLAYRRDGKVFAAGTRDRRVWFGGEGRTSKLASLELDDRVWAVAFSPDGQ
                     TLLTGIEKGR"
     gene            complement(3026..3655)
                     /locus_tag="VHS97_07125"
     CDS             complement(3026..3655)
                     /locus_tag="VHS97_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3448009.1"
                     /translation="MRSEYPDLVALPPEAQGPSFDDFMRRIRAGDESAALELVERYEP
                     AIRRAVRVRLRDRRLRRLVETVDICQSVFASFFVRTSLGQYDLETPDKLVRLLVTIAR
                     NKVAHQANKEHAACRDQRRINPAAAIGDLPAPGGSPSRQLAARELVAEARKRMTEDEL
                     LLLERREQGLGWAAIASELGGSPDGQRVRLARAVARVSKELGLDEGPDE"
     gene            3893..4057
                     /locus_tag="VHS97_07130"
     CDS             3893..4057
                     /locus_tag="VHS97_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448010.1"
                     /translation="MGNLIVGTDGTVDPTSFGVGGGVFTVGATTIDNTTITGNHASSN
                     FNDVDGTFST"
     gene            complement(4186..5142)
                     /locus_tag="VHS97_07135"
     CDS             complement(4186..5142)
                     /locus_tag="VHS97_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3448011.1"
                     /translation="MKDATMSRRLFLGGTVASGALLTLGSRVALAAAESGKPAPGTIG
                     DFKISLAEWSLHRALRAKMVDNIDFPRIAREEYGCEGVEFVNQFFKDKAHDSTYLKEL
                     KKRANDFGVTCVLIMIDGEGDLSATQDDRRKTAVENHKKWVDAAAALGCHAIRVNTGD
                     HYSPTDVTAVAEACGSLTDYGAKNGISVICENHGGPSSNPDALLALMKAVGKPTFGTL
                     PDFGNFPHDKAGLYTVDVYDAIARMMPYAKGVSAKSYDFDESGKETKLDYARIMKIVT
                     DAGYHGFVGIEYEGDRLGEPEGIRATKKLLESVRGSTYSPGK"
     gene            5341..5613
                     /locus_tag="VHS97_07140"
     CDS             5341..5613
                     /locus_tag="VHS97_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448012.1"
                     /translation="MRLPSALFKIRGLAVLTAVAAAVLGASILLIRNSGTTRTSAPPR
                     PNKGYLIMEGVDINWMRPGHLTPDRPSSGQRLSPPDFQGTGARSER"
     gene            5775..6227
                     /locus_tag="VHS97_07145"
     CDS             5775..6227
                     /locus_tag="VHS97_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3448013.1"
                     /translation="MAGRRVLAITERQFGVLRVLWEHGPLTVRGLMEHLPRGDQQPYT
                     TVLGLMQTLEKARLVEHEKEGLTHRYRPLVSRQEATGSLLSDFLSRFFHGSAEQLILG
                     LVDAEHLSPDDLGAIEARLVKASQSRGSAATSEDSQQVIKPRRGRKKP"
     gene            6224..>6736
                     /locus_tag="VHS97_07150"
     CDS             6224..>6736
                     /locus_tag="VHS97_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448014.1"
                     /translation="MTLLEFTDRWVVPGLGFLADWSVRWGILLAILLLWLVARPPRRA
                     STRYLMTILVLTSGALLPAVPRWARIAISWPSTATIVARQESPHSTTSTGPADAAADV
                     ISTEEPSPRRGAMTATTAGPRPQSAAPTTDVAGRPSLGRWQWTALVIAAAWTSVVLVL
                     TCRLVGGMIVL"
BASE COUNT         1223 a         2121 c         2065 g         1327 t
ORIGIN      
        1 cggccctttt cgattcccgt gagcagagtc tgtccgtccg ggctaaacgc aaccgcccag
       61 acccggtcgt ccagttcgag cgacgcaagt ttcgatgtgc ggccctcacc tccaaaccag
      121 acccggcggt cgcgcgttcc cgcggcaaat acctttccgt cccgacggta agccagtgaa
      181 aggaccgggc cgagatgatc gagcgtgaca tcgggctggc ggtccgttgt tgtgctccac
      241 gacaggactt ttgctgcact tccgccaacg ccggtaagga acgattctcc gtcctgcgga
      301 tggaatgcca ccgcagtgac ccagcccttg tgcctcaggc ggcgctcgcc tgaacggatc
      361 ggcgccgtcg accagatccg gatagcgctg tctcggcctc gagtcaaaac ggccttgccg
      421 ttagggctga acgacgcatc gaggacttcc ttgacgtgag gcaagggggc gcccaacatt
      481 tcgccggtct ctgcatccca gaggcggacc tgatctgaac tcaccgtgag cgtgacgaga
      541 ccgttcgagc tgaaaccgac agtcttgacg gcatctgggt ggcgcatgat cgcgcgcgaa
      601 gtcgcgttgt ccgagaggtc ccacaatcga gcgagtcggt cctggccgcc gatgagaatc
      661 gtcttgccgt cagggttgaa cgccactgat cggatcgccc ccgagtttcg gaacacgggt
      721 cccaggggcg caccgctttt actgtcccaa agccggcagg tccgatcgta gctgccagtc
      781 acaaaagaat ggccatcgtt cgggctaaag gcaaccgcgg cgacccggct ggtatgttcc
      841 ttcagggcac ggacgatcct gccgtcggcg gttctccaca ggcgggcgcc cacctcccgg
      901 acctcgcttg ccgtgaccaa cgcctcaccg tcggggctga atgtcgcgag cgtgattccc
      961 ctgcccagct cgatctcggc tccggcgcgc tgaccggtgt caatcagcca tgactgaagc
     1021 caggcagtct ggccccgccg agacgtcgtc cagagccgat gaccgtctcg gctgaaaacg
     1081 tacgagagca gttcggcccc tttgcccaga tccaatcctt tctcgaaaac tggcttaccg
     1141 ctagccgtcg cccagagccg aatctcttga ccggctgcgg tcaagaccat cgccccgtcc
     1201 gggctgaagg cggcgaccgt agcggatcgc ggcaggcgga tcgtccgggc cggaccgtcg
     1261 ccgcctgcga catcccagac gcgagccgat ttctcggcgt cggttgtgag gacgaagcga
     1321 ccgtcggcac tccacaccgc cgcgaggacc cgggacgagt gatcaaggac tgactggagc
     1381 gggtgaagcc tgccctgcca tcctgcaaga ttggtgcgaa ttgcgcgttt aaggttgtcg
     1441 tcgtcgtccg gaacgagttt aaggctctgg gcaagccaga gtagcccgcg gccgacgtca
     1501 ccatcgtcgc aatggcgcag ggcgcggtcg cggagcaaga cggacgagat tccttgatat
     1561 aagcgtcgct gctgcgactc gctctcccgg gccgattccg ccgcgatccg ctgcgccttt
     1621 tcagccttct ggtctcgctc cgcgcgtctg agcgcttgtc gtgcctcttg tgccttcccg
     1681 gcgattgccg cgaccgcgac aatcgcgccc agcgagtaga ccgccagaag cgccgccaga
     1741 cgaggacgac gccgcgccca gcgaatagcc cggctgattg cgccaatggg ccgcgcatgg
     1801 actggctcgc catccaggaa gcgacgcaaa tcgtcagcca agtccgccgc gcttgaatag
     1861 cgccgggagg gctctttgct caaacacttg agacacactg tctcgaggtc gcgcggcact
     1921 ttcggctgga accggcacgg cgaaaccggt tcctcgtgaa ccacctgcgc aagggtgtcc
     1981 agcacggatt cgcctttgaa cggtgggcga cccgtgagca tatcataaag aattgcgccg
     2041 agcgagtaga catccgccgc gggcccgacc tccttgctct ggccggttgc ctgttccggt
     2101 gccatataac tgggggaacc gataatttcg ccagttactg tgcggccgag gccgccgacg
     2161 gtgagcttag ccaggccgaa atcgacgatc ttgggctcgc cccgcctgct aaagagcaca
     2221 ttgctgggcg tcaggtcgcg gtggatcaca ccccggtcat gggccgcgtg ggtagcgcgt
     2281 gccagcgtct ccaccaacgc ggctgcgcgg cgggacgcca aaggtgtgcc tcttaagact
     2341 tccgccagcc ggccgccgtc cacatactcc atcgtgaagt agggatagcc atcgagctcg
     2401 ccgatctcgt agaccttgac gaggttcggg tgctcgagcc gagcgacagc cgcggcttcg
     2461 ccccgaaacc gcgcccggtc gcgcggctgg gagtattcac ccgccaagat catcttcacg
     2521 gccaccaatc gattcaggcc ggtttgccac gcgaggtaga cgacgcccat tcccccacgg
     2581 ccaagctcgc gaatgacctc gtggccccgg atcgacggac cggtaccggt cgccacccag
     2641 ctctcgggtt ggacagagct acgccaggac gcatccgaat ggcgggccga gccgagcaag
     2701 tcagtgagat tgagtccgtg atgaacctca aactgatctc gaagttgacg cgcgaagagt
     2761 gggaaacggt ggagatactc ctcaagctgc ggtttctcgc ctaactcctc gcgaataatg
     2821 acttcgtagt agatgaggtc cagaacgtcg tcctcgcccc ggattgtctc ggtaagcgtt
     2881 ggcaggaaag tttccacgag aacgcgctcg ccacgcgccc agcggcggcg ctgctcgtct
     2941 tgcacgcgac gaacgtcgtc gccggcggat ctgaaaccac gcgtggtgac ttcgctatct
     3001 gcgtgttcta gcggttgttg atctatcact catcgggccc ttcgtcgagt ccaagctcct
     3061 tggagacacg tgccaccgcc ctggcaagac ggactctctg tccatcgggg ctcccgccga
     3121 gctcgctggc gatcgcggcc caccccaggc cttgctcacg tcgttccaag agcaggagct
     3181 cgtcctccgt catccgcttt ctcgcctcgg ccacgagctc tcgtgccgcg agttggcggc
     3241 tggggctacc gcctggcgct ggaagatcgc cgatcgcggc ggcgggattg atccgccgct
     3301 gatcccgaca tgcagcatgc tctttgttcg cctggtgagc gaccttgttg cgggcgattg
     3361 tgacaagcag ccggaccagc ttgtccggtg tttccagatc gtactgtccc agcgaggtcc
     3421 ggacaaagaa agacgcaaag accgactggc aaatgtcgac cgtttctacc aggcgccgca
     3481 gccgtcggtc gcgcagcctc acccgcacgg cccgccggat cgccggttcg taccgctcaa
     3541 ctagctccag ggcggcactt tcgtctccgg cccggatcct gcgcatgaag tcgtcgaaag
     3601 atggcccttg cgcctccgga ggcagcgcaa cgagatctgg gtattccgat ctcatgactg
     3661 ggtccctctc gtgctcgatc gtcgagtatt cacgcacgtt tcatcaggga aaaaccattg
     3721 tacccgcgtt cttccacgat cgtcttcatg tttttttcac gaacttgttc ggtctgatcg
     3781 ccagccgcga tagaccccat gatgcgagct caggcgccgc cgcgcgacgc ggtttcgctc
     3841 cttcatttcg tcattgacag caggcttgat tctcagtgag ggggtgtttg atgtgggtaa
     3901 cctcattgtg gggaccgacg ggactgttga cccgacaagc tttggcgtcg gcggcggtgt
     3961 tttcaccgtc ggcgctacga ccatcgacaa cacgacaatc accggcaacc acgcgtcgtc
     4021 gaatttcaac gatgtggacg ggacattctc gacctgagga agaatgacta cgacacgaaa
     4081 ggcccccggc cgacggcacg cagatcgtgc gcactatcag gcagggggcc tttggtccat
     4141 taattcaggt gtcaatggcg cgattacatt ctcgggcgcg tcaaatcact tgcccggcga
     4201 atacgtgctc ccgcgcaccg attccagcag ctttttggtt gcgcggatgc cttccggctc
     4261 gcccagccgg tcgccttcat actcgatacc gacgaagcca tgatagccgg cgtcggtaac
     4321 gatcttcatg atccgggcgt agtcgagctt ggtctctttg ccggattcat cgaagtcgta
     4381 gctcttggcc gagacaccct tggcatacgg catcatccgg gcgatcgcgt cgtaaacatc
     4441 aaccgtgtag agccctgctt tgtcgtgggg gaagtttcca aaatcaggaa gcgttccaaa
     4501 ggtcggcttg ccgacggctt tcatgagcgc gagcagggcg tcggggttgc tggacggccc
     4561 gccgtgattc tcgcagatga ccgagatgcc gttcttggcg ccgtagtcgg tcaaactgcc
     4621 gcaggcctcg gcaaccgcgg tgacatcggt ggggctgtag tggtcccccg tgttcacccg
     4681 gatcgcgtgg cagccgagag ccgccgcggc atcgacccac ttcttgtggt tttcgacagc
     4741 ggttttgcgc ctatcgtcct gcgtcgcgct caagtctccc tcgccgtcga tcatgatgag
     4801 cacgcacgtg acgccaaagt cattggctcg tttcttgagt tctttcaagt acgtggagtc
     4861 gtgagccttg tccttgaaaa actggttgac aaactcgacc ccttcgcacc cgtattcttc
     4921 acgagcgatc ctgggaaaat cgatgttgtc aaccattttg gcacgaaggg ccctgtggag
     4981 cgaccactcg gccagtgaga tcttgaagtc tccaatcgtg ccgggcgccg gcttgccgga
     5041 ttcggcagcg gccaaggcca cgcgcgagcc gagagtcagc aaggctccac tggcgaccgt
     5101 gccgcccagg aacaggcgac gactcatcgt ggcatctttc atcgaaccag tctccttgct
     5161 cgcgatcact cttaatgtgc ggaaaacatc gacaagctcg aatcttatcc ggaagatatg
     5221 gacgattcaa cgatcgtgat cggacgggca gtgctgggat ggaatggatt gccacacggg
     5281 gcgggtgtaa gctttggagg agcgatcatc ctatccagtc aaccaaaaat ggagagggcc
     5341 atgcgacttc ccagcgcgct tttcaaaatc cgcggtcttg cggttttgac ggcggtcgca
     5401 gccgccgtat tgggagcttc gatcctcttg ataagaaatt ccggcacgac ccgaacctcc
     5461 gcgccgcctc ggcccaacaa gggctatctc atcatggagg gggtagacat caactggatg
     5521 aggccgggtc acttgactcc ggatcgaccg tcgagtggac agcgactctc gccgcctgac
     5581 ttccagggta ctggcgcacg gtcagaaagg taaagcgaag taagaacggg ccgaccaagc
     5641 gcatggccgt gttacttcgt gtcatccgcg cgcactggat cagcaccgtc gatcgtcgac
     5701 ttgacgccac gctctgtcct acgcaataat ccgtacgttg tcgcacgtcg aaatatttac
     5761 ggaggttttt actgatggcc gggcggaggg tgctggcgat tacggaacgg cagtttggcg
     5821 tattgcgggt gctctgggag catgggccgc tgaccgtgcg tgggctgatg gaacacctgc
     5881 ctcgcggcga tcagcagccg tacacgacgg tgctcgggct catgcaaacc ttggaaaaag
     5941 ctcggttggt cgagcacgag aaggagggct tgactcatcg ataccgtcca ctcgtatcgc
     6001 gtcaggaggc gacgggcagt ttgctttccg atttcttgag ccggtttttt catggctcgg
     6061 ccgagcagtt gatcctgggc ctggtcgacg ccgagcatct ctcgcccgat gacctggggg
     6121 ctatcgaggc acgcctggtg aaagccagcc agagtcgtgg ctcggcggcc acgtccgaag
     6181 attcccagca agtgatcaag ccccgacgcg ggaggaagaa accgtgactc tcctcgaatt
     6241 taccgatcgc tgggtcgtgc cagggctggg gtttctggcc gactggtcgg tgcgctgggg
     6301 cattttgctg gcgatactgc tcctctggct ggtcgcgaga ccgccgcggc gagcctcgac
     6361 ccgttatctc atgaccattc tcgtgctgac gtcgggggcg ctacttccgg ctgtaccgcg
     6421 ctgggctcgt atcgcgatct cttggccgtc gacggcgacg atcgtcgccc ggcaggaatc
     6481 tccccattcg accacgtcga cgggaccggc ggatgccgcg gccgacgtga tttcgaccga
     6541 agagccatcg ccgcgacggg gcgcgatgac ggcgacgacc gcaggcccga ggcctcaatc
     6601 cgccgcgccg accacggatg ttgcaggacg gccgtctctt gggcgctggc aatggacggc
     6661 actcgtaatc gccgcggcgt ggacttccgt cgtcctcgtg ctcacttgcc ggctggttgg
     6721 cggcatgatt gtgctg
//
LOCUS       DATAQL010000413         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1262309, whole genome shotgun sequence.
ACCESSION   DATAQL010000413 DATAQL010000000
VERSION     DATAQL010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262309"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..915)
                     /locus_tag="VHS97_07155"
     CDS             complement(16..915)
                     /locus_tag="VHS97_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3448015.1"
                     /translation="MVRPSGLKIGALAVCMGMVSGVTVKAAPPKLTIDQDIAYSKVGS
                     RELKLDIARPAEGDGPFPAVLVIHGGAWRQGNKADVRPILPQFVEHGFVAISPEYRFC
                     PQNAFPAQIHDVKAAVRWLKVNAKKYRIDPDRIGAMGFSAGGHLAMMLGLTSPNDGLE
                     GDVSAGAPDSRVKAVVNYFGPTDLGAKDIPDICKPLVKDFLGGSPQDRPDAAAKASPL
                     TFVSKDDAPVLTFQGTKDSLVPYNQAIKLAEAMNSAGVPGRVELILGADHGWAGAEMA
                     RTVNETFGFLDRYLKAEPSHAKP"
     gene            complement(932..1939)
                     /locus_tag="VHS97_07160"
     CDS             complement(932..1939)
                     /locus_tag="VHS97_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3448016.1"
                     /translation="MKIVIYPAVEPTRLEALKGAAPELEFVNAATAALAEASMPGADA
                     MLGKISPAMLARADRLRWVQSFTASLEHYIFPELVNHPCVLTNARGLFGDVIADQVMA
                     YVLCFARNLHTYVRRQMEHRYEPCGGETARVSNLSGPGTVNAMDKATIYLPRATMGII
                     GMGAIGCEIARRAVSFGMKVRAVDRFPERVLAPEGVQSVDGIDCLADVLAFSDFAVIA
                     APHTPETAGLFNAATLAHLRPKSYLINIGRGAILVLDDLVAALKAKKLAGAALDVYEI
                     EPLPADHPLWDFPNVILTPHTAGYSPVIAERHLAVLTENVGRFARGERLINVVDKALW
                     F"
     gene            complement(2507..>2734)
                     /locus_tag="VHS97_07165"
     CDS             complement(2507..>2734)
                     /locus_tag="VHS97_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448017.1"
                     /translation="AEFRSLDRALRDWGGGVPVSSRDFRALKLDRALERALSIEPGPA
                     SVPADGAKQIADHLSNVCRKSRGRSASVAPI"
BASE COUNT          553 a          821 c          838 g          522 t
ORIGIN      
        1 ttgatcctta ccggttcagg gcttggcgtg gctcggctcg gccttgagat aacgatcgag
       61 aaaaccaaac gtctcgttga cggtccgcgc catctcggcc cctgcccagc cgtgatcggc
      121 gcccaggatc agttcgacac gaccgggcac tcctgccgaa ttcatggcct cggccagttt
      181 gatcgcctgg ttatagggaa ccagtgagtc tttcgttccc tggaatgtga gcaccggcgc
      241 atcgtccttg gaaacgaacg tcagcggcga cgccttggct gcagcatcag gtcgatcttg
      301 cggcgaaccg cccaggaaat ccttgaccaa gggcttgcag atatcgggaa tatccttggc
      361 gcccaggtcc gtcggaccga agtaattgac gacggctttg acgcggctgt cgggagcacc
      421 cgccgacaca tctccttcca gaccgtcgtt cggacttgta aggcccagca tcatggccag
      481 gtgccctcct gccgaaaaac ccatcgcgcc gattcggtcc gggtcgattc tatatttctt
      541 ggcattcacc ttcagccagc gcacggcggc tttcacgtcg tggatctgcg ccgggaacgc
      601 gttctgggga caaaagcgat actcgggcga gatcgcgaca aaaccgtgct cgacaaactg
      661 gggcaagatc ggccgtacgt cggccttgtt cccctggcgc caggcgccgc cgtgaatcac
      721 gagcacagct ggaaatggtc cgtcgccttc tgccgggcga gcaatatcga gcttgagctc
      781 ccttgagcca accttgctat aggctatgtc ttggtcgatt gtcagcttgg ggggcgcggc
      841 ctttaccgtg acccctgaaa ccatgcccat gcagacggcc agagcgccga tcttgagacc
      901 cgacggacga accatggtgg caactctccg attaaaacca caaagccttg tccacgacat
      961 ttatgagtcg ctccccgcgc gcgaaccggc ccacgttttc agtaagcact gccaggtgcc
     1021 gttcggcaat gacgggcgag tagccagcgg tatgcggggt caggataacg ttgggaaaat
     1081 cccagagcgg gtggtcggct ggcaagggct cgatctcgta gacatcgagc gccgcgccgg
     1141 cgagcttttt ggcctttaaa gcggcaacca ggtcgtcgag caccagaatt gctccgcgac
     1201 cgatgttaat caaatagctt ttggggcgaa gatgagccaa tgtcgcagca ttaaacaaac
     1261 ctgcggtctc gggcgtgtga ggggcggcga tgaccgcgaa gtcgctgaag gccagcacat
     1321 cggcgagaca atcgatccca tcgaccgact gcacaccctc gggcgctaag acacgctccg
     1381 gaaaacggtc gacggcacgc actttcattc cgaagctcac ggcgcgccgg gcgatttcac
     1441 agccaatcgc ccccatgccg atgatcccca tggtggcgcg tggtagatag attgtggcct
     1501 tatccattgc gttgaccgtt cccggaccgc tcaagttgct gacccgcgcc gtctcgccgc
     1561 cgcatggctc atagcggtgt tccatttgcc ggcgaacata cgtatgtagg ttgcgcgcga
     1621 aacagagaac atacgccatt acctggtcag cgatgacgtc gccgaagagt ccgcgggcat
     1681 tggtgagtac gcaggggtgg tttaccaact cggggaagat atagtgctcg aggctggctg
     1741 tgaacgattg gacccagcgc agccgatccg cccgagccag cattgcgggg gaaatcttcc
     1801 cgagcatggc atcggccccg ggcatggacg cctcggcaag ggcagcagtg gccgcgttga
     1861 cgaattccag ttcgggggca gcgcccttga gcgcctcgag ccgcgtgggc tcgacggccg
     1921 ggtagatcac gatcttcatg gcggcaattc tcgcaataac gattcgaact cgctgtaagc
     1981 ccgcgagcaa gtcagactat tgtgatcggc ccgtttggtc cgtgccagct agtaagaatg
     2041 gccagtacac agcgggtgcg caaacggatc gtagcgagcg cggggattcc agagtggatt
     2101 gcgctctgac accgttctag cgctctatca ggttgcggat cgcgtcttcg tcccaaaggg
     2161 acagaaacat gacagcccag ggcagcgccc tggggaatcg acgccaaacc aatcaggcga
     2221 gccctgaaag ggcgtgacaa ggaaccagtc cacgcctcga ttattcgcgg ggacggtgac
     2281 ccgcatgatg ggatcgaacg cgattcaagg ccctttcagg gcaggagtgt tttgtctgtt
     2341 tatggccacc cagggcgttg ccctgggctg gcatgtgatt gtcccttcag gacgaaaatc
     2401 gggatgctca acctccgccc tggcgtgtga gaatccccgc ccgacgtctc tatgctctgc
     2461 cgcgccaggg atgccagaat ccattggcgt acccgtgcac tgtcgatcag atcggggcga
     2521 ccgacgccga gcggccgcgc gattttcgac agacgttcga gaggtgatcg gcaatttgtt
     2581 tggccccgtc ggcagggacg gaagcaggac caggctcgat ggagagcgcc ctctcaagtg
     2641 ctcgatcgag cttgagcgcg cggaagtcgc gcgatgagac cggcactccg cctccccaat
     2701 cgcgcaatgc ccgatcgagc gaacggaatt cggc
//
LOCUS       DATAQL010000414         7410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1262566, whole genome shotgun sequence.
ACCESSION   DATAQL010000414 DATAQL010000000
VERSION     DATAQL010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262566"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..97
                     /locus_tag="VHS97_07170"
     CDS             8..97
                     /locus_tag="VHS97_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3448018.1"
                     /translation="MAAAVLFLCSPLAGYITGHVLDVDGGFLG"
     gene            240..1175
                     /locus_tag="VHS97_07175"
     CDS             240..1175
                     /locus_tag="VHS97_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448019.1"
                     /translation="MAEDARREPSSSDPDPEIAAAEWLFRDESGAKPKQPPDSKVAPG
                     SSTNEVFDLAEGPSSEDLLERRPIGPPTGAGATGQTSAAREGQTRQTHDQSRLDPSAL
                     VNEVWSRWSEWGGTLILVLGWLAILFTIVYFILGWDQYGIAFLTLILGGIAAVILSYP
                     ILITLERPVRITPEQALRDYYGSLSHHFPHFRRMWLLLSSAGRISTAYGSFEGFKAYW
                     KDRLGQLRQGHAGSLTPLVFEVVDFKADKSAGKVRIEAQFALKVWVRGRRRDGTIHSF
                     PMSMALVRGPDNMWYLEDGTLGRDRQTARSATAAE"
     gene            1296..1595
                     /locus_tag="VHS97_07180"
     CDS             1296..1595
                     /locus_tag="VHS97_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448020.1"
                     /translation="MGRTRYQFGESPFPHFLTCAVAGWLPVFTRPETVQIVLDSWQFL
                     QDQDRRVLFGYVVLENHIHFIGCRHNRLVIQRLETTRSVEDGIPTRSMGTSQTFD"
     gene            complement(1693..3360)
                     /locus_tag="VHS97_07185"
     CDS             complement(1693..3360)
                     /locus_tag="VHS97_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3448021.1"
                     /translation="MLFRDNPVLTRELLVTLRSPRSFVLQLLYVCALGALVYFRWPAG
                     EEGARQVSPGVARALYDIFFLGQFFLVALMAPTFAAGSITGEKERKTYELLLASPLRP
                     STILVGKLLSSLSYLVILIISSLPLMILCYLLGGLLLSEITRSYLVLILAAGTFGLVS
                     VACSSYFRRTSSALVVSYLVILPLAVLCVVLTGTDNVVARDFVSIAILPPWCLAAWTV
                     LGILVNRRLLHPPDVGSEGKDVVDEVEEMKYAIGVVIDRDLFPDKLFAPAKRTDLMPD
                     GTNPVLDKELRSEIFSQGTLMLRVVIQVSMLLSIPLMAALLFLRSDKAGYYVAYVLAF
                     NLLVGPVFSAGSITQERERQTLSLLLTTLLRPGQIVFAKLMAALRVSTVLTFLLTEQL
                     LLAYILLPELRQRFWTLLVFLLIIATTCMATSTIGLMCSSLARRTSTAMVMTYLTLLT
                     LFVLPLGVQSYLQTIASVSEEQLAALTVTSPFAAALGVPMHNTRTEAWTNQPLAVADP
                     ILIPIAAKFQLPVWAIFLCLYPPLGLIFFWITYLAFRWRWWRAGGLG"
     gene            complement(3381..4310)
                     /locus_tag="VHS97_07190"
     CDS             complement(3381..4310)
                     /locus_tag="VHS97_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3448022.1"
                     /translation="MIEVINFTKRYGDFVAVDNLSFSIGKGEIFGFIGPNGAGKSTTI
                     RFLATLLRPTAGEGRVAGFSVTAEPMSVRRVIGFMPDDFGVYDGMKVWEFLDFFAVAY
                     EIPRSYRKKIIGEVLDLLDLTHKRDDYVNGLSKGMKQRLCLAKTLVHDPPVLILDEPA
                     SGLDPRARLEMKALLNELKQMGKTILISSHILSELADFCTSIGVIERGKLLAAGSIQE
                     IQARIRSHRVLKVRVLEETTDGAAEILRLDESIRMVETYDHTLTAEFEGEDRDMGRLL
                     DRLVSSGIHVQSFAEEPLSLEEVFMMITKGIVS"
     gene            complement(4349..6952)
                     /locus_tag="VHS97_07195"
     CDS             complement(4349..6952)
                     /locus_tag="VHS97_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448023.1"
                     /translation="MKAITFRVRIALFLAGLFACFGASASAQITPSEEERLQILTEPE
                     SIAKKVAKDKNQAPFEFFRSQVAPFDVLPFVKANHWCTVLFELRANLDNYDGGLQTNP
                     VMLVGMPQQVAFHREARLLKEKRANLALQVMLPSASGQVPKEWAVEMVRPNSLRPDAS
                     WQVNLTTLPPHQMLVLILSKDSTTKFTAWNRMSAIIPSSVERDAGDIEKQRYYRLVLP
                     IEPDKPAISSHPLTWSTISHTIWDGYSPDTLTFSQQQALLDWLHWGGQLIVSGGAGQS
                     FSLLRESFLGPYLPADASGENVALSQDDLQPLSQSYPPPAAPAGLEDQSKPTPLSGRE
                     ALRRFGSHYQAPVPIRPAPKRPVYLSVLRPRPGSSTIPLGDASPHLIAVESRVGRGRI
                     TMLTLNPNEDALLAWPGLDTLVRRVVLRRPEEPLAGGGNYQNPDARGPERGRLLAADL
                     SWYRITSRDAGLEATMPSRLEPGPESTRPRPTVDPEGPDAIENHLNRIPSVGEWRDGA
                     RIPRLSRDLLEDASGITIPSSRFVLQVIIAYLLAVIPLNWLICRFILNRREWTWAVVP
                     LVALAFAIGVERVAARDMGYEAACDEIDLLEIHGDYARAHLTRLASLYTTGRSRFAIS
                     YPNDPTALALPFDSGRSIRGEEVSSSTWQSYPVPSLVDFSVQPRSLSMFRAEQMLALG
                     GPIRLEGDAGKRQVVNSSDLELRDAILIDLANPNERRERWLGTIAAGASIDIDGPAHE
                     EPPKRFETGPGPDANPFLKELRTTREPSERNQGELRLVAWMAGTTAGQVIEPAVDRRR
                     GFTAVMVHLRSGSPPSPDGMVYNRLAGGEPAVEPSYPTRKKSSRPVSGTRSTAPRIPA
                     SRPPGSPKSNG"
     gene            complement(7142..>7410)
                     /locus_tag="VHS97_07200"
     CDS             complement(7142..>7410)
                     /locus_tag="VHS97_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448024.1"
                     /translation="MVYDPWLYPVFESIGLAEPRSIWLEQAHSTGPVRVVVSTTSRPR
                     IEGLERTLHELGYEDAIRVGDYFIWKKQARGGEQARAPVDDIVK"
BASE COUNT         1550 a         2230 c         2219 g         1411 t
ORIGIN      
        1 tgacgaagtg gctgccgctg tgctgtttct ctgttcgcca ctggccggtt acatcactgg
       61 ccatgtcctg gacgtcgacg gcggatttct gggctaattc cctttttagg agtcagcccc
      121 acggcgcgtc cgccagcctg accgttcggg aactcaatcg catctcggac cactgcaggt
      181 tacaattcgc ccattggccg gtggccgtgc ctcgtgaatg acgggatgag aggattgtaa
      241 tggccgagga cgcaaggcgc gagcctagtt ccagtgaccc cgaccccgaa atcgccgccg
      301 ccgagtggct gtttcgtgac gagtccggcg ccaagccaaa gcaaccgccc gattcgaaag
      361 tcgctcctgg ttccagcaca aacgaggttt tcgacctggc cgagggtccg agctccgaag
      421 acctgctaga gcgacgtccg attggtccac caacgggcgc cggcgcaact ggccaaacga
      481 gcgcggcccg cgagggccaa acacgtcaga cacacgatca atcccggctc gatccgtcgg
      541 ccctggtcaa tgaagtctgg tcaagatggt ctgagtgggg gggtacactg atcttggtgc
      601 tgggttggct agcaatcctt ttcacgatcg tctacttcat cctggggtgg gaccagtacg
      661 ggatcgcctt tctaaccctt atcctgggcg gtatcgcggc ggtaatcctg tcctatccga
      721 tattgatcac gctcgaacgg ccggtgcgga taacccctga gcaggccctt cgcgactact
      781 acgggtctct ttcccaccac tttccccatt ttcgccgcat gtggctgtta ctcagctcgg
      841 cgggccggat ctcaacggcc tacggttcgt ttgaggggtt caaggcgtac tggaaagatc
      901 ggctcggcca actgcgccag ggtcatgcgg gatcgctcac tcctctggtt tttgaggtcg
      961 tcgatttcaa ggccgacaag agcgcgggca aggtccgcat cgaggcccag tttgccctga
     1021 aagtatgggt gcgaggccgt cgccgggacg ggacgatcca ttcctttccg atgtcgatgg
     1081 cgctggtacg cgggccggat aacatgtggt atctggagga tggaaccttg gggcgcgatc
     1141 gtcaaacagc caggtcggcc accgctgccg aatgacccta gtgtcccgtc agccttcgat
     1201 tgacgggtgg tcttctaaca caagttccca tgctctgtgt gggaatgcag tctgcgacgc
     1261 tccgcgtcgt cttctcgagc caagaaccac gacccatggg acgcacccgc tatcagttcg
     1321 gagaatcccc tttcccccac ttcctgacct gcgcggtcgc tggctggctg cccgtcttca
     1381 ctcgacccga gaccgttcaa atcgtgctcg attcgtggca gtttctccag gatcaggatc
     1441 gccgggtgct gttcggatat gtcgtcctcg agaaccacat tcacttcatc ggttgccgtc
     1501 acaaccgact ggtgatccag cggttggaga cgacgcggag cgtcgaagac ggcattccca
     1561 cgcggagcat gggaacgagt caaaccttcg attgacgggt ggtcttttcg aagggctaat
     1621 gacgcaaaga atgcgaactg cactggcaac gaccccggcg ggttgacccg tcaatcgaag
     1681 gttgacgcca cactagccga gcccaccagc acgccaccac cgccatcgga acgccaggta
     1741 ggttatccaa aagaaaatca gtcccagagg cgggtagaga cagaggaaga tcgcccagac
     1801 tggcaattgg aacttggcag cgatcgggat caggattgga tcggcgaccg cgagaggctg
     1861 gttggtccaa gcttcggtgc gcgtgttgtg catcggcacg ccgagggccg ccgcgaacgg
     1921 gctggtgacg gtcagggcgg ccaattgttc ctcgctgacc gaggcgatcg tctggagata
     1981 agactgaacg cccaagggga ggacgaagag ggtaagcagc gtgaggtaag tcatcaccat
     2041 cgccgtcgag gtccgccgcg ccagcgacga gcacatgagt ccgattgtcg acgtcgccat
     2101 gcatgtggtg gcgatgatga gcaagaacac cagaagggtc caaaaccgct ggcgcaactc
     2161 gggaagcaag atgtatgcaa gcagtagctg ctcggtgagc agaaaggtca gtaccgtgga
     2221 tacgcgcaat gccgccatca gcttcgcgaa gacgatctgg ccgggccgca gcagggtcgt
     2281 gagcaggaga ctcaaggttt gccgctcgcg ctcctgggtg atgctgccgg cggaaaacac
     2341 tggtccaacc agcaggttga acgcaagcac gtaggctacg tagtagccgg ccttgtctga
     2401 ccgcaggaaa agaagcgcgg ccatgagcgg aatcgacaac agcatgctga cctggatcac
     2461 cacgcgcagc atcagggtgc cctggctaaa gatttcgctt cgtagctcct tgtcgaggac
     2521 cggattggtg ccgtcgggca tgaggtctgt ccgtttggcc ggggcgaaca gcttgtccgg
     2581 gaacaagtcg cgatcgatca ccactccgat cgcgtacttc atctcttcca cttcgtcgac
     2641 gacgtccttt ccctcactgc cgacgtccgg aggatggagc aagcgccggt tgaccaggat
     2701 ccccaatacc gtccatgctg ccagacacca gggagggagg atcgcgatcg acacgaagtc
     2761 gcgagccacc acgttatcgg tgccggtcaa gaccacacat aagacggcga ggggaaggat
     2821 caccaaatag ctgacgacca gagccgagct ggtgcggcga aaatagctcg agcaggcgac
     2881 ggagaccagc ccgaacgtgc cggccgccag aatcaaaacc aggtagctgc gcgtgatttc
     2941 agacagtaac aatcctccca gcagatagca gaggatcatt aaaggaaggc tggatatgat
     3001 cagaatcacg agataggaaa gcgagctcaa gagcttacct accaggatcg tggacggtcg
     3061 gagcgggctc gccagcagca gctcgtaggt cttccgctct ttctcgccgg tgatgcttcc
     3121 cgcggcaaac gtcggcgcca tcaaggccac cagaaaaaac tgcccgagga agaaaatgtc
     3181 ataaagcgcc cgcgcgacgc ccgggctgac ttgtcgagca ccttcctccc cggccggcca
     3241 gcggaaatag accaaggctc ccagcgcgca gacgtaaagc aactgcagca caaacgaccg
     3301 cggcgatcgc agtgtgacca agagctcacg ggtcaagacc gggttatcgc ggaacagcat
     3361 ctgagtttcg agtctctcct ctagctgacg attcccttgg tgatcatcat gaagacttcc
     3421 tcgagcgaga gcggttcctc ggcaaacgat tgcacgtgga ttcccgaaga cacgagccgg
     3481 tcgagcagcc ggcccatgtc acggtcttcg ccctcgaatt cggcggtgag ggtatggtcg
     3541 taggtttcca ccatacggat tgactcatcg aggcggagga tttccgcggc cccgtcggtg
     3601 gtttcttcga gcacgcgcac cttgagcacc cggtgcgagc ggatgcgggc ctggatttcc
     3661 tggatactgc cggcagctag caacttgccg cgctcgatga cgccgatcga tgtgcagaaa
     3721 tcagccagct ccgagaggat atggctcgaa atcaggatgg ttttacccat ctgtttgagc
     3781 tcgttcaata gcgccttcat ctcgagccgc gccctggggt cgaggcccga ggccggctcg
     3841 tccagaatga gaacaggcgg gtcgtggacc agtgtcttgg ccaggcagag ccgctgtttc
     3901 atccctttcg acagcccgtt gacgtagtcg tcacgcttgt gggtcagatc gagaaggtcg
     3961 agcacttcgc cgatgatctt cttgcgatac gaccgcggga tctcgtaggc gaccgcgaaa
     4021 aagtcgagaa actcccatac cttcatgcca tcataaacgc cgaagtcgtc cggcatgaag
     4081 ccgatcacgc ggcgaaccga catcggttcg gcggtgaccg agaaaccggc gacgcgtccc
     4141 tcaccggccg tgggtcgcaa gagcgtcgcc agaaaccgga tcgtcgtact cttgcccgcg
     4201 ccgttgggcc cgataaagcc gaagatctca cccttgccga tggagaaact gaggttgtct
     4261 acggcgacga aatctccata gcgtttggtg aagttgatca cttcgatcat gaatccacct
     4321 actccgtcgt tatcggtgac tttcgctctt acccatttga cttgggactt cccggcggcc
     4381 gagacgccgg aatccggggc gctgtcgacc tcgtgcccga tacaggcctc gaggatttct
     4441 tccgagtcgg atagctaggt tccacggcgg gctcaccgcc ggctagccgg ttgtaaacca
     4501 tgccgtccgg actcggcggg ctgccgctac gcagatgaac catgaccgcc gtgaacccgc
     4561 ggcgccgatc gacggccggc tcgatcactt ggcctgctgt tgtccccgcc atccacgcaa
     4621 ccagtcgtag ttcgccctgg tttcgttcgc tgggctccct cgttgttcgc agttccttca
     4681 agaacggatt ggcatccggt cccgggccag tctcaaacct cttgggaggc tcttcatgag
     4741 cgggaccgtc gatatcgatc gaggcacccg cggcaatcgt acccagccag cgctcgcggc
     4801 gctcgttcgg attcgccaga tcgatcaaga tcgcgtcgcg gagctcgaga tcgctactgt
     4861 taaccacctg gcgtttgccc gcgtccccct ccaaccggat cggaccgcca agggccagca
     4921 tctgttcggc cctgaacatc gagagactgc ggggctgaac tgaaaagtcc accagtgacg
     4981 ggacaggata cgattgccag gtcgagctcg aaacctcctc gccgcggatc gaccggccgc
     5041 tgtcaaacgg caatgccagc gcggtcggat cgttggggta cgagatcgcg aatcgcgatc
     5101 gaccggtggt atatagcgac gccaaccgcg tgagatgagc gcgagcgtag tcgccgtgaa
     5161 tttcgagcaa gtcgatctcg tcgcaggccg cttcgtagcc catgtcgcgc gctgccaccc
     5221 gttccacgcc gatcgcgaag gcgagcgcaa ccaacggcac gactgcccac gtccattcac
     5281 ggcgattcaa tataaaccga caaatcagcc agttgagcgg aatgacagcg agcagatagg
     5341 caatgatgac ctgtaggaca aaccgcgagc tgggaatcgt gatgccggag gcatcttcga
     5401 gcaagtctcg cgaaagtcgg ggtattcttg ccccgtcacg ccattcgcct acgctcggaa
     5461 tgcggttcaa gtggttttca atggcgtcgg ggccttcagg gtcgacagtc ggacgggggc
     5521 gggtcgactc aggtcctggc tctagccgac tcggcatcgt cgcctccaga cccgcgtcgc
     5581 gactcgtgat tcgataccag ctcaggtcag cggcgagcaa tcggccgcgt tccggacctc
     5641 gggcgtccgg gttttggtaa ttacctccgc ccgcgagcgg ttcttccggc cggcgcagca
     5701 ccacgcggcg aaccagagtg tcgaggccag gccaggccaa caaggcatct tcgttgggat
     5761 tcaaagtcag catggtaatc cgtccgcgcc cgacccggct ctcaacggca atcagatgag
     5821 gactcgcgtc gcccagggga atcgtcgagg atccaggcct gggccgcagg actgatagat
     5881 acacgggacg tttcggagcc ggccggatcg gtacgggcgc ctgatagtgc gatccaaatc
     5941 gccgcagggc ctcgcggcca ctcaacggcg tcggtttgct ctgatcttca aggccggccg
     6001 gggcggccgg cggaggatac gactgcgaga gaggctgcag gtcatcctga gaaagcgcga
     6061 cgttctcgcc ggacgcatca gccggcaagt acggacccaa aaaactctcg cgcaagagtg
     6121 aaaacgattg acccgctccg cccgaaacga tcaattgacc accccagtgc agccagtcga
     6181 ggagcgcttg ctgctgcgag aacgtcagcg tgtcgggcga ataaccatcc cagatggtgt
     6241 gactgatggt tgaccaggtg agagggtgtg atgaaatcgc gggcttgtcc ggttctatcg
     6301 gcaagacgag ccgataatag cgctgcttct cgatgtcacc agcatcgcgt tcaaccgacg
     6361 atgggatgat cgccgacatc cgattccacg ccgtgaactt ggttgtcgag tctttactca
     6421 gaatcaaaac gagcatctgg tgcggcggaa gcgtcgtcaa gttcacttgc cagctcgcgt
     6481 caggccgcaa cgagttgggt cggaccatct caacggccca ctctttcggt acctgaccgc
     6541 tcgccgaggg gagcatgacc tgaagagcca ggttggctcg cttttccttc aagagccgcg
     6601 cctcgcggtg gaaagccacc tgctgtggca ttccgaccaa cataacggga tttgtttgaa
     6661 ggccgccgtc gtaattgtcg aggttggccc ttagctcgaa gagcaccgtg caccagtgat
     6721 tcgctttcac aaacggtagc acgtcgaacg gagcgacctg cgatcggaaa aactcgaaag
     6781 gggcctggtt cttgtctttc gcaaccttct tcgctatcga ctcgggctcg gtgagaatct
     6841 gcagccgctc ttcttcacta ggggtaatct gggccgaagc ggatgcgccg aaacacgcga
     6901 aaagccccgc caagaacaag gcaatccgaa ctcgaaatgt gatcgccttc atggactgcc
     6961 gccttcaaag ccagccgcca gcgacgtccg gctgaaacct aatcccggcc gcttcgattt
     7021 cgacaccgag ggaattaatc taataggttc cacgagtgta acacctgtgg acccgcaacg
     7081 gaagggtcgc gaaatcgcaa tcgcgagtcg tgaaatctct tcgacctttt cagaccggaa
     7141 ttcatttcac aatgtcgtcg accggcgccc ttgcctgctc tccgccacga gcctgctttt
     7201 tccagataaa atagtccccg actctgatcg catcctcata accgagctcg tggagcgtgc
     7261 gctccaaacc ctcgattctc ggacggctcg tcgtcgaaac gacaacgcgc accgggcctg
     7321 tggaatgggc ctgctcgagc cagatcgatc gtggctcggc caggccgatc gactcaaaaa
     7381 cggggtaaag ccacggatca taaaccatgt
//
LOCUS       DATAQL010000415         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4469172, whole genome shotgun sequence.
ACCESSION   DATAQL010000415 DATAQL010000000
VERSION     DATAQL010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4469172"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2375)
                     /locus_tag="VHS97_07205"
     CDS             complement(<1..>2375)
                     /locus_tag="VHS97_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448025.1"
                     /translation="RVLCMPYFGGADLARVLKTAGGLVPTHHDGVSLVEALDQISLKL
                     PECSAVVQTHRPVRISRASSLKKDEPAASPPAVLIATPAMGSAATSRLRGLLSRFVWS
                     AAHSPLHQRDLAAERDEPSRQFLRGASAIQAAVWIMAQLSEGLEHAHARGLLHRDLKP
                     SNILLAADGTPMLLDFNLAVEQVADSPEDEIRRAIVGGTLPYMSPEHLDAFNPNGTTP
                     AHAVDERSDIYAMGLILFEMLTGESPFPEPPPGTQLIETMDLMVACRRQPPSLRSVCP
                     QVPWSLDALTAKCMAFDSARRYARARDLAEDLRRFLDNLPMKHCPEPSFRERAGKWAR
                     RHPGLCGSTSIAVFALVLLGVLGATTALVFDRMQSLSARVRYRIFDQEFSDIQFLLNT
                     AGGSNEHLKTGLEKAAHALEPFRVDNGDTPKHGDWAQRLTADERRRLSEQVVELIMLD
                     ARAHVLLASQQGTPEDRRRAFQRAIKRLDHTERTHAGAPSALYAERARYHAALGEAEL
                     ARRDRERSRQIAPSTCHDLTLLATSLLAGGDRMGAEQALRQALRLDVTSFWAWFVLGH
                     CHYAQGRFLEAAGDFAACAVRGPQFAWVHFNRGLALARAGRPLEASHAYDRALELDPQ
                     FSEALVNRAMAQLELNQLTAAERDLLRAVELGRDDLVTLTALGETLSRSGRQAEAERC
                     FADLLSKRPGSAVARIARGFSRIASNPAGAQSDLSQALGEEPRNAQALYGMAVLARGG
                     DLREALNYLNRALAIDSNLIDALQLRALVRAQLGDLAALDDIERLLQSPTS"
BASE COUNT          432 a          717 c          808 g          418 t
ORIGIN      
        1 cgaagtcggg ctttgcagaa ggcgctcgat gtcgtcgagt gcggccagat cccccagttg
       61 cgcccgaacc agggcacgca gttgcaatgc atcgatcaaa ttggaatcga tcgctagagc
      121 tcgattgagg taattgagcg cttcgcgcag gtcccctccg cgcgccagga ccgccatacc
      181 gtagagtgcc tgtgcattgc gcggttcttc gccgagtgcc tgtgagagat cactctgggc
      241 gccggctggg ttggatgcga tgcggctgaa gccgcgggcg atgcgtgcga ctgcggatcc
      301 cggtctcttc gaaagcaggt cggcgaaaca gcgttcggct tcggcttgac gtcccgagcg
      361 cgacaaggtt tcgcccagtg ccgtcagtgt gaccagatca tcgcggccta gctcgacggc
      421 acgcaagagg tcgcgctcgg ctgcggtcaa ctggttgagc tcgagctgcg ccatggctcg
      481 gttgaccaat gcctcggaaa actgtgggtc gagctcgaga gcccgatcgt acgcatgact
      541 ggcctcgagt ggccggccgg cgcgcgccag cgccagccca cggttgaagt gcacccacgc
      601 gaactgcggg ccgcgcactg cacatgcggc aaagtcgccg gctgcttcga ggaatctccc
      661 ttgcgcatag tggcaatgtc cgagaacgaa ccatgcccaa aacgaggtga catcgagtcg
      721 caacgcttgc ctgagagctt gctccgctcc catccgatcg cccccagcga gaaggctcgt
      781 cgccaagagc gtcaggtcgt ggcaggtgga aggtgcgatc tggcgggacc gctcgcggtc
      841 gcgacgggcg agctcggctt caccgagcgc cgcgtgatag cgcgctcgtt cggcgtaaag
      901 ggcggatggc gctcccgcgt gtgtgcgctc ggtgtggtcg aggcgcttga tcgcgcgttg
      961 gaatgcacgg cggcggtcct ctggcgtgcc ttgctgactg gcaagcaaca cgtgggcgcg
     1021 cgcatccagc atgatcaact cgacgacttg ctcactcagg cggcgtctct cgtcggcggt
     1081 caaccgctgc gcccagtcac cgtgtttcgg ggtatccccg ttgtccacgc ggaatggctc
     1141 gagggcgtgc gcggctttct ctaatcctgt tttcagatgc tcgttcgagc cgcccgcggt
     1201 gttaagcagg aattggatgt cgctgaattc ttggtcgaag atccgataac ggacccgcgc
     1261 gctcagcgat tgcatccggt cgaaaaccag ggccgtggtc gcgcccagca cgcccagcaa
     1321 tacaagggcg aaaaccgcga tcgacgtcga gccgcacagg ccaggatggc gcctggccca
     1381 cttgcccgcg cgttctcgaa agctcggctc agggcaatgc ttcatcggca ggttatcgag
     1441 gaagcggcgc aggtcttcgg ccaggtcgcg tgcccgggcg taccggcgag ccgagtcgaa
     1501 agccatacat ttggcggtca gcgcgtcgag gctccaagga acttgagggc agacggaacg
     1561 caacgacggc ggttgacggc ggcaggcgac catgagatcc atggtttcga tcaattgggt
     1621 acccggcggc ggctcgggga atggcgattc gccggtcaac atctcaaaga ggatcagccc
     1681 catggcgtaa atgtccgacc gctcgtcgac cgcgtgggcg ggagtcgtgc cgttggggtt
     1741 aaacgcgtcg agatgctcgg gagacatata tggtaatgtg ccgccgacaa ttgcgcggcg
     1801 gatctcgtct tccggcgagt cggcgacttg ttccactgcc aggttgaaat cgagcaacat
     1861 cggcgtgccg tcggccgcca gcaaaatgtt cgacggcttc aggtcccggt gcaagagtcc
     1921 tcgggcgtgg gcatgctcga gaccttcgga gagctgggcc atgatccaca cggcggcctg
     1981 gatcgcgctt gccccgcgca agaactggcg cgacggctcg tctcgctcgg cggcaagatc
     2041 gcgctggtgg agcggtgagt gcgcggctga ccagacgaac cgggatagca gcccgcgcaa
     2101 gcgggaggtc gccgccgaac ccatggcggg ggtcgcgatc aagaccgcgg gcggggaagc
     2161 cgcgggctcg tcctttttga gacttgacgc acgggaaatg cgaactggac ggtgagtctg
     2221 cacaacggcc gagcactcgg gtaatttcag gctgatctga tcgagggctt cgaccaggct
     2281 cacaccatcg tgatgtgtcg gaacaagacc gccggcagtc ttgagaactc tggccaggtc
     2341 ggcacccccg aaatacggca tgcagaggac gcgca
//
LOCUS       DATAQL010000416         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4926759, whole genome shotgun sequence.
ACCESSION   DATAQL010000416 DATAQL010000000
VERSION     DATAQL010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4926759"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VHS97_07210"
     CDS             complement(<1..587)
                     /locus_tag="VHS97_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448026.1"
                     /translation="MKHDVDREEPVVMDIADVVTRFDHHDPVIMADPTETYRRFRNEC
                     PVGRSHVHDGFWVVSRYDDVLHVAGDAEQYCSGQGIAIPGTSTEARIEPQEADGPRHA
                     QIRKQMNAWFARGPVLAMRPAIEDLSASLLEQCSARVTFDLAADYATPLASLTMLRVL
                     GVPIEYQGRLRKNLDVLLHARTAEESVVFGAYIEL"
     gene            complement(584..1135)
                     /locus_tag="VHS97_07215"
     CDS             complement(584..1135)
                     /locus_tag="VHS97_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3448027.1"
                     /translation="MCSCDERAIERILFRYARCVDSADWDCLGELFRYGEVVTEGVDG
                     VAVGPDAVKDVWRTVNRVHPDGTLRTRHLLTNVIVDVSPDDDIANADAYFMVFQATPE
                     LPLQPIAGGWYEDTFHKIDGMWWFKQKKIHVNLVGDVSNHLILAFDSSGQLSDPPPAD
                     LLAAASGRAPADPANSPNPEPAT"
     gene            1270..2166
                     /locus_tag="VHS97_07220"
     CDS             1270..2166
                     /locus_tag="VHS97_07220"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3448028.1"
                     /translation="MTRPALSAARSVDVLNFMASRPRESFTLSELSRQLGISTASGHA
                     VLQVLTAANYLVRDPVQKRYRLGPTLIALGHSALEAHPAIDRARDEVRHLADSLQLEC
                     LAEIVCDDEIMILARAGRPRKFEFLPRVAQRLPLIPPVGNALCAWADEPTTKKWLDRL
                     GPIPKQEMARITQAIEGIRARGYDVGLETPTRKQIGELLARHAHDAESKDWKPVLADL
                     FKTLAREENQIVGPIDPCREYLVNYIMAPAVNDRGEALVCLTVLGFEGALSGQEIQKI
                     GEVVVQAGALAGIAGREASTYP"
     gene            2212..>2618
                     /locus_tag="VHS97_07225"
     CDS             2212..>2618
                     /locus_tag="VHS97_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3448029.1"
                     /translation="MHATLSIGDFARATHLSVKTLRHYHQIGLLEPADVDRHTGYRRY
                     TTAQIPVAQVIRRFRDLDMRLDQIGAVLAAPDPEARNRLIATHLNRLEDGLSRTQMAV
                     ASLRDLLEGPAGSGTRHVGRRRLEATRAAAIIEV"
BASE COUNT          515 a          804 c          792 g          507 t
ORIGIN      
        1 tataactcga tatacgcacc aaatacgacg ctctcttccg ccgttcgcgc gtgcaagagt
       61 acgtccaggt tctttcggag gcggccttgg tattcgatcg gtactccaag aactcgcaac
      121 atggttaacg acgccagggg cgtcgcatag tcagccgcga ggtcgaaagt gacgcgcgca
      181 ctgcactgtt ccagcagaga ggccgataga tcttcgatag ctggccgcat cgccagcact
      241 ggaccccgag cgaaccacgc gttcatctgc tttcgaatct gtgcatgccg ggggccatcg
      301 gcttcttggg gctctatccg cgcttccgtc gaagtgccgg gtatcgcgat tccctgaccc
      361 gagcagtact gctcggcatc accggccaca tggagcacgt cgtcgtagcg cgagaccacc
      421 caaaagccat cgtggacatg cgaccggccg acggggcatt cgttcctgaa gcggcgatag
      481 gtttcggtgg gatccgccat gattaccgga tcgtggtgat cgaaacgagt gaccacgtct
      541 gcaatgtcca tgacgaccgg ctcctcacgg tcgacgtcgt gcttcatgta gccggctccg
      601 gattcggcga gttcgcagga tcggccggcg cccggccgga agctgcggca agcaggtcgg
      661 caggcggggg atcgctgagc tggccggagc tgtcgaaggc caaaatgaga tggttgctca
      721 cgtcgccgac caggttcacg tgaatcttct tctgcttgaa ccaccacatt ccgtcgatct
      781 tgtggaacgt gtcctcgtac catccgcccg cgatcggttg cagcggaagc tcaggggtgg
      841 cttggaaaac catgaagtac gcatcggcgt tggcgatgtc gtcgtcaggg gatacgtcga
      901 cgatgacgtt ggtgagtaga tgccgagtcc gtaatgtgcc gtcgggatgc acccggttta
      961 cggtccgcca gacgtctttg acggcgtccg gacccacggc taccccgtca acaccttcgg
     1021 tcaccacttc tccgtaccgg aatagctcac cgaggcaatc ccagtcggcc gaatcgacac
     1081 atcgcgcgta gcgaaacaag attcgctcga tggctcgctc atcgcagcta cacataagtc
     1141 tccttcggta aactgttgtc gtaatatgga gaagggtctc gcgattgcga gatgatcgta
     1201 ggtgggatac ttggtccgcg caagccaatg cgcgggtcca accgcgacga aaggaaccga
     1261 ccaccaccca tgacccgacc tgctctctct gctgcgcggt ccgtcgacgt cttgaacttc
     1321 atggcttcca ggccgcgcga gtcatttacg ctctcggagc tgtcgcgaca gctcggtatc
     1381 agcacggcat caggacatgc cgtgttgcaa gtccttacgg cggcgaacta tctcgtacgc
     1441 gatcctgtac agaagcgcta ccgtctcggc cccacgctga tcgccctcgg acattccgcc
     1501 ttggaagcac atccggcgat tgaccgggcc cgcgacgagg tccgacacct cgccgattcg
     1561 ctccagctcg agtgtctggc cgagatcgtc tgcgacgacg agatcatgat cttggcgcga
     1621 gctgggcggc cacgaaagtt tgaattcctt ccgcgggttg ctcagcgtct cccactgatt
     1681 cccccggttg gcaatgcgct ttgtgcatgg gccgacgaac ccaccacgaa gaagtggctc
     1741 gatcgactcg gcccgatccc gaagcaagaa atggcccgca ttacgcaggc gatcgagggt
     1801 atccgagccc gcggctacga cgtgggtctt gaaacgccca cacgaaaaca aatcggtgag
     1861 ctgttagccc gacatgcgca cgatgccgag tcaaaggact ggaagcccgt gctcgccgat
     1921 ctcttcaaga ctctcgcgcg tgaagagaac cagatcgtgg gcccaatcga cccatgtcgg
     1981 gagtacttgg tcaactacat catggctcca gcggtcaacg accgcggcga agccttagtg
     2041 tgcctcacag ttctcggctt cgaaggtgca ctgagcggcc aggagatcca gaagatcggc
     2101 gaggttgttg tgcaagccgg cgctcttgcc ggaattgccg gacgcgaggc gtcgacctac
     2161 ccgtagaacc tttcgtgact ctccccgtgg gggagtgtgc agacttgagc catgcacgcg
     2221 acgctttcta tcggtgactt cgcgcgagcg acccatttga gcgtcaagac gctacgtcac
     2281 tatcaccaga tcgggttgct tgagccggcc gatgtggacc gccacaccgg ttaccggcgg
     2341 tacaccacgg cgcagatccc ggttgcacag gtgatcaggc ggttccgcga tcttgatatg
     2401 cggctggatc agatcggggc ggtgttggca gccccagacc cagaagcacg gaaccggctg
     2461 atcgcaactc atctcaaccg cctggaggac ggcctgagtc gcacccaaat ggcggttgcg
     2521 tcattgcgag atttgctcga gggtcccgcc gggtcgggca cccggcacgt cgggcgacgc
     2581 cggctggagg ccacacgggc agctgcgatc atcgaggt
//
LOCUS       DATAQL010000417         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3554141, whole genome shotgun sequence.
ACCESSION   DATAQL010000417 DATAQL010000000
VERSION     DATAQL010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554141"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VHS97_07230"
     CDS             <1..453
                     /locus_tag="VHS97_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448030.1"
                     /translation="WAVLLVIATAWLIPLAEPYRSSRILGEKLASLSAKLKIEPVLLE
                     YQEPGVVYSLGHPIATTRDRDGFFAHLAGGKSVLTVALPSEVEVMRSHFGLIVTTVDQ
                     VEGFIMAKGKKQTLQLAVVRESDRPIAEPTLGLNTRRVGLKLENTLVK"
     gene            complement(397..1578)
                     /locus_tag="VHS97_07235"
     CDS             complement(397..1578)
                     /locus_tag="VHS97_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3448031.1"
                     /translation="MRIVHVITRLIIGGAQENTLLTVQGLDHRYHDDVTLITGPAEGP
                     EGDLFDRATQLGLKVLVFPELVRAIRPVVDLQAYRRLRAAFRRLEPDVVHTHSSKAGI
                     LGRAAAWHEKVPAVVHTIHGMPFGKFETALKNRLYITLERWAARRCHAIVSVCDAMTE
                     QALAAGVGRPEQFLTVYSGMNADAFLDPPRPRADVRRELGLGDDEVAFATVARLFELK
                     GHDDIVAVAAPVLAANPKVRFVWIGDGILRDRLIADLERRGIRGSFILTGLVPPDRIP
                     ELLNAVDAVIHPSLREGLARVIPQAMLVGRPVISYDIDGAREVVLPETGILLEPRDLD
                     GLRQAILSLAASPSTRAAMGREGRARFADQFRHETMTRQLRSLYERILELQSHPARVQ
                     A"
     gene            complement(1662..>2133)
                     /locus_tag="VHS97_07240"
     CDS             complement(1662..>2133)
                     /locus_tag="VHS97_07240"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEX3448032.1"
                     /translation="GAEEKPQAFHATLDAKSKRYRYVIDNGAFANVFQLRYSWHVRRA
                     LDDTAMARAGRFLLGRHDFHSFETEWPNRTSSVRTIFDLTVERAESVVTIEVEADGFL
                     YNMVRSIAGTLVLVGASKRSEEWVAEVLAAENRVMAGPTAPAKGLFLVKVCYAP"
BASE COUNT          401 a          667 c          682 g          383 t
ORIGIN      
        1 tgggccgtcc tgcttgtgat cgccacggcc tggttgattc cactggccga gccgtaccgt
       61 agctcgcgca ttctcggcga gaagctggcg tcgctctcgg ccaagctcaa aatcgagccg
      121 gtattgctgg aataccagga acctggggtc gtttattcac tcggacaccc catcgcgacc
      181 acccgcgatc gtgacggctt cttcgctcat ctggcgggag gcaaatcggt tctcactgtc
      241 gctctcccca gtgaggtcga ggtcatgcgc agccattttg ggctaattgt gacgaccgtc
      301 gatcaggtcg aggggttcat catggccaag ggaaaaaagc agacgcttca actcgccgtg
      361 gtgcgcgaaa gcgaccggcc catcgcagag cccactttag gcttgaacac gcgccgggtg
      421 ggattgaagc tcgagaatac gctcgtaaag tgacctgagc tgccgggtca tggtctcgtg
      481 gcgaaactgg tcggcgaacc gggcgcgtcc ctcgcgtccc atcgcagccc gcgtgctggg
      541 actggccgcg aggctgagga ttgcctgacg caggccgtcc aggtcgcgcg gttctagcag
      601 gatgcccgtc tccggaagca cgacctcccg ggcaccgtcg atgtcgtagc tgatcacggg
      661 gcgtcccacc agcatggctt ggggaatgac ccgcgccagc ccctcgcgga ggctgggatg
      721 gatgacggcg tcgacggcat tgagcaattc gggaatccgg tcgggcggca ccaggccggt
      781 gaggatgaaa gagccccgaa ttccgcgccg ctccaggtcg gcgatcagcc ggtcgcgcaa
      841 aataccatcg ccgatccaga cgaaccggac cttggggttg gccgcgagca caggcgcggc
      901 gacggcgacg atgtcgtcgt gtcccttgag ctcgaacagt cgcgcgacgg tggcaaaggc
      961 gacctcgtcg tcgccgagcc ccagctcgcg ccgcacgtcg gcacgaggcc gcggcggatc
     1021 gagaaatgcg tcggcattca taccgctata taccgtgagg aattgctctg gcctgccaac
     1081 gccggctgca agcgcctgct cggtcatcgc gtcgcatacg ctgacgatcg cgtggcatcg
     1141 ccgggccgcc cagcgctcga gtgtaatgta gaggcggttc ttgagtgccg tttcgaactt
     1201 cccaaacggc attccgtgga tcgtgtggac gaccgccggc actttctcgt gccacgcggc
     1261 ggcgcgcccg aggatgcccg ccttggagct atgtgtgtgg acaacatcgg gctcgagccg
     1321 acgaaaggcc gcgcgcagcc gtcggtaggc ttgcaagtcg acaacgggcc ggatcgcccg
     1381 gaccagctcg ggaaaaacca aaaccttgag gccaagctgg gtggcgcgat cgaagagatc
     1441 gccctcaggc ccttcggccg ggccggtgat caaagtgaca tcgtcgtgat accgatggtc
     1501 cagaccttgg acagtcagga gagtgttctc ctgagcgccg ccgatgatca gcctggtgat
     1561 gacgtgaacg atccgcattt cagcgtggac tcgcagtcgt ggaggcgacg tgccgtggtc
     1621 ccacaacggc ggacaccgca ttgcaaatgc gttttcgccg actagggcgc atagcaaacc
     1681 ttcacgagaa ataggccctt tgccggcgca gtcgggcccg ccatcactcg gttctcggcc
     1741 gcaaggactt ccgcgaccca ctcttccgag cgtttactag cgcccacgag caccaatgtg
     1801 cccgcgatgg accgcaccat attgtacaga aagccgtcgg cttcgacttc gatcgtcacg
     1861 accgactctg cccgctcgac ggtcagatcg aagatggtgc gaacgctgct ggtgcgattg
     1921 ggccattcgg tctcgaagct gtggaagtcg tgccgaccca ggagaaaccg cccagcgcgg
     1981 gccatcgcgg tatcgtcgag agcacggcga acgtgccagc tataccgaag ctgaaatacg
     2041 ttggcgaacg cgccgttgtc aatcacgtaa cgatagcgct tcgacttcgc gtcgagtgtc
     2101 gcgtgaaatg cctgaggctt ttcctcggca ccc
//
LOCUS       DATAQL010000418         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_194048, whole genome shotgun sequence.
ACCESSION   DATAQL010000418 DATAQL010000000
VERSION     DATAQL010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194048"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VHS97_07245"
     CDS             <1..827
                     /locus_tag="VHS97_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX3448033.1"
                     /translation="RDGQRQDGRCHGQRQDGQAVELRCRANARRLVARHEALLTADLM
                     GLVPMMRLEAKHSLATRIFHWANAPILAVMIWSGLLIYWANDIYAIRLGGITLIEFFP
                     DWFYRFFNVGHRLAEGMAWHFTFMWLFGLNGLLYVLYTVWSGEWRFLLPNRATFAEAW
                     QVVRHDLGLRKEPLPRRKFNGAQRLAYTGVVAMGAGSVLTGLAIYKPVQLAWLTAFMG
                     GYPLARLEHFVLAVGYVAFFVIHISQVIRAGWNNFRAMVIGVEVSNDQEAIHGTAT"
     gene            811..1731
                     /locus_tag="VHS97_07250"
     CDS             811..1731
                     /locus_tag="VHS97_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3448034.1"
                     /translation="MERPLDQQPMADLADPRENNMVECSADVRPASPLAADGTPTPQV
                     AIRPIDSTIGLTRPDGDLERRLHRLSRRGFALGGAAALAGVVGWRWLVTRGEEGGLAW
                     PLRRALEFDERLARGLFRQSSLSPQFPRSAARMPRVNGSIGLDANLDQARWRLQVIGS
                     AGEHSPQSFTLDQIRAFPRVEMTTELRCIEGWSDVVHWAGARLANLASVTGLATHGAR
                     RLGYAALQTPDGQYYVGLDMASALHPQTLLCYEMDGRPLTPEHGAPLRLVIPVKYGIK
                     NLKQIGTIRFTDVRPADYWAEQGYDWYAGL"
     gene            1817..>2767
                     /locus_tag="VHS97_07255"
     CDS             1817..>2767
                     /locus_tag="VHS97_07255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3448035.1"
                     /translation="MDAIRFKLILILGTILIPLSTAPPVVAQDQDAGHCPGKLEALNA
                     SYVQQLRDIERRWIAELADLAGKSGGPGTNDAYRQLFNLAIARDLCIDAQPAARNCLG
                     SIPEGEDVRALAASVQVFARAENGEYEQSLADLEALFKEPGCVAQSAAKSHAATALAV
                     GEAYLKRLIRDGRYDVAHRLCALACKDDATAALKNHFAARMARLDLLDKPAPAISGTD
                     ADGHQVSLADLKGKVVLVDFWESGCSQCVASMSALEALAQKYHRRGFVILGVNIEEKH
                     RDGKDAKTALQTARQVLVRHSASWINLLDCQRTENITKAYG"
BASE COUNT          500 a          847 c          893 g          527 t
ORIGIN      
        1 ggcgcgatgg acaaaggcaa gatggacggt gccatggaca aaggcaagat ggacaggccg
       61 tcgaactgag gtgccgagct aacgcccggc ggctcgtggc ccgacacgaa gcgttgttaa
      121 ctgccgactt gatggggctc gtaccgatga tgcgactgga agcgaaacac tcgctggcga
      181 cgcggatctt ccactgggcc aacgcgccga ttctcgccgt catgatctgg agcgggctct
      241 tgatttactg ggctaacgat atttacgcga tcagactcgg gggaatcacg ctcatcgagt
      301 ttttcccgga ctggttttac cggttcttca atgtcggcca ccggctggcg gaagggatgg
      361 cgtggcattt cacattcatg tggctctttg gattgaacgg tctcctgtat gtgctctata
      421 cggtttggag cggcgagtgg cgtttccttt tacccaaccg tgccactttt gcggaggcat
      481 ggcaggtcgt gcggcacgac ctggggctga gaaaagagcc gctgccgcgc cgcaagttta
      541 acggagcaca gcggctggcc tatacgggag ttgtggctat gggtgctggc tcggtgttga
      601 ccgggctggc gatttacaag cccgtgcagc tggcgtggtt gaccgcgttt atgggcggct
      661 acccgctggc gcggctcgag cattttgtcc tcgccgtcgg ttacgtcgcc ttctttgtga
      721 tccacatcag ccaggtcatc cgtgctgggt ggaacaattt ccgcgcgatg gtcatcggtg
      781 tggaggtgtc gaacgaccag gaggccattc atggaacggc cacttgatca acagccgatg
      841 gccgatcttg cggacccgcg cgaaaacaat atggtcgagt gcagtgcaga cgtccggccc
      901 gcttctcctc tggccgcgga cgggacgcca acaccacaag ttgcgattcg tcccatcgat
      961 tcgacgatcg gcctcacgag gccagacggc gacctggagc gtcgcctgca ccgactgtcg
     1021 cgccgcggct tcgccttggg tggcgcggcg gcactggccg gtgtggtggg ttggcgctgg
     1081 ctggtcaccc ggggcgagga gggagggttg gcctggccgc tccgccgcgc tttggagttc
     1141 gacgagcgac tggctcgggg cctgtttcgc cagtcgagtc tgtcgccgca attcccccga
     1201 tccgccgctc ggatgcctcg cgtcaacggc tcgatagggc tggacgcgaa cctcgatcag
     1261 gccaggtgga ggctccaggt catcggatcg gccggcgagc attcaccgca gtcgtttacc
     1321 ctcgaccaga tcagggcctt tcccagggtc gagatgacga ccgagctccg ctgcattgag
     1381 ggctggagtg atgtcgtgca ctgggccggc gcgcgactcg cgaacctcgc ctcggtcacc
     1441 ggtttggcga cccacggagc acgtcggctc ggctacgccg cgctccagac acccgacggc
     1501 cagtactacg tcggcctcga catggccagt gccctccatc ctcagacttt gctttgttac
     1561 gagatggacg gccggcccct gacccccgag cacggcgcgc cgctccggct ggtaatacct
     1621 gtcaagtacg gaatcaagaa tctgaagcag atcggaacca tccggttcac tgacgttcgg
     1681 cccgccgact actgggccga acagggctat gactggtatg cgggactctg aaactcgggc
     1741 aacgacgttc gccgtcgggc gctctgattc accatattgt gtagatacct gacttcgcca
     1801 gagaaaggag cccatgatgg acgcgatccg gttcaaactg atcctgattt tgggcacaat
     1861 cctgatccct ctttcgactg ctcctccagt cgtcgcccag gaccaggatg cgggacactg
     1921 ccctggaaaa ctcgaggcgc tcaatgcctc atacgtccag cagttgcgcg acatcgagcg
     1981 ccgctggatc gccgagcttg ccgacctcgc gggcaagtcg ggcggtccgg gaacgaacga
     2041 cgcctaccgg cagttattta atctggcgat cgcgcgagac ctttgcatcg acgctcagcc
     2101 cgcggcccgg aactgcttgg ggtcgatccc cgaaggagag gacgtccgcg cgctggccgc
     2161 atcggtccag gttttcgccc gagccgagaa cggggagtac gaacagtcgc tcgccgactt
     2221 ggaggcacta ttcaaggaac cgggttgcgt ggcgcagtct gccgcgaagt cgcacgcggc
     2281 gaccgcgctc gccgtgggag aggcctacct caagcgcttg atccgagacg gccgctacga
     2341 cgttgcccac aggctgtgtg cgctggcctg taaggacgac gccacggccg ccctgaaaaa
     2401 ccacttcgcg gcccgaatgg cccggctcga tctgctcgac aagcccgcgc cggcgatctc
     2461 gggcaccgac gccgatggcc accaggtatc cctggccgat ctcaagggga aagtcgtcct
     2521 ggtcgatttc tgggagtcgg ggtgctcgca gtgtgttgcc tcgatgagcg ctctggaggc
     2581 cttggcccaa aagtaccacc ggcggggctt cgtcatcctg ggcgtcaaca tcgaagagaa
     2641 gcatcgggac ggcaaggatg ccaaaacggc cctgcaaacc gctcgccagg tcctggtcag
     2701 acacagtgcg agctggatca atctgctcga ctgccagagg acggagaaca tcaccaaggc
     2761 ctatgga
//
LOCUS       DATAQL010000419         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_346554, whole genome shotgun sequence.
ACCESSION   DATAQL010000419 DATAQL010000000
VERSION     DATAQL010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346554"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="VHS97_07260"
     CDS             complement(<1..2053)
                     /locus_tag="VHS97_07260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3448036.1"
                     /translation="MNSRLSVASANGPSRTVDLVSSPINDRRVVAALEDYGELMRGGS
                     PPTRAEFLALHHSIADILADPLAGLELAQAAAGDLAPTGSEHDPAEDTLLSATLGEYR
                     IIREVGRGGMGIVYEAEQVPLGRRVALKVLPSTASLDPRHRQRFRIEAQAAALLHHEH
                     IVPVFAIGCDRGVDYYAMQFIDGQSLNERIRSALQMPPQYDRVEPTTTVGVGPRRRSL
                     HSPFPPRQPGSSLGDRRHCKAAAQLALQAAFALEHAHEIGVIHRDIKPSNLLIDVRDH
                     LWIADFGLARLPQDEHDLTRTGDLVGTLRYMSPEQVRGDRASVDARTDLYALGVTLYE
                     LLTLRPAFSGRNRDELVRRILDGDPVPPRRINPSIPRDLETIILKAMEKEPSARYRTA
                     RALADDLRRFLDDEPIRARRPTVVDRAVKWSRRHRTATIASTIALLVTLTISTAVLCV
                     SNRRTDDLLRRTDALLDKNRVARLEGEEAFQRSLGTLDQIIRSVSSESDPSKTETAKR
                     VYPWAIEFYTFIGRRFSESESISKEVLAKAHRQAGFCRLNMGDRRGRDDYRHAIRGFQ
                     DVAARFPDRLWYRTRLIETLLEYAGLLPAPADAALADELFRSALRVAEALLGDKNADL
                     HCYRIGLIGPFNDLAWKLVRRAPTRESDAALAIRLARKAVNCEPGQAAYWNTLGVAYY
                     RQ"
     gene            complement(2159..3670)
                     /gene="pyk"
                     /locus_tag="VHS97_07265"
     CDS             complement(2159..3670)
                     /gene="pyk"
                     /locus_tag="VHS97_07265"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX3448037.1"
                     /translation="MHKEKLGAVRTKIVATVGPASSNPAVMKQMVDAGVDVFRLNFSH
                     GTHEVHTASLEAIRRIADETERQLAVLQDLCGPKIRLGEVHGGVVVCDQDAEFVLTAE
                     PGDGSDAHHLTSTYRSLPDDLDVGQSVLFADGTVAMDVVARGPGWARLKVTLPGHIRS
                     NQGINVPRAGLSVGALTDKDLVDLDWTAAHEVDYVGLSFVRKADDIALLRRELERRGS
                     RARIIAKIEKPQALANLDAIVAEADAVMVARGDLGVELDVIRVPAIQKQIIGACHRAR
                     VPVITATQMLNSMESSSRPTRAEASDVFNAVLDGTDAVMLSGESAIGEYPVESVSMMS
                     RIAGEAEKLLFSEFRTGAPWTWSVANWPGAEAARDGSTQNNGVARAGRVQPITESVVE
                     AASLISRRLSAALLVVATSSGRTALVLSKQRNSAPTLAIAHDPQTARAMALYWGVTPL
                     PRPEIASPDELRAFILDWCREKGLIAPGDCIVGIRGSRSDDPTHNEIVVLEVR"
BASE COUNT          828 a         1223 c         1244 g          792 t
ORIGIN      
        1 cttggcggta ataggcgaca cccagcgtgt tccaatacgc ggcttggccg ggctcacaat
       61 tcacggcttt gcgagctagc cgaatggcca gagcagcgtc agattctcgc gtgggagcgc
      121 gacggaccag cttccaggcc agatcgttaa acggtccgat cagcccgatc cgatagcagt
      181 gcaaatcggc gttcttgtca ccgagcagag cctcggcgac ccggagtgcg gagcggaaca
      241 gctcatctgc tagggcggcg tccgcgggcg ccggcagcag accggcatat tccagaagcg
      301 tctcgatcag acgagtccgg taccataacc gatcggggaa tctcgccgcc acatcctgaa
      361 agccacgaat cgcgtgccga taatcgtcgc gtccgcgccg atctcccatg ttcagtcgac
      421 agaaaccggc ctggcgatga gccttggcta gcacctcttt tgaaatactt tcgctttccg
      481 aaaatcttcg gccgataaac gtgtaaaact cgatcgccca tggatagact cgcttggccg
      541 tttctgtttt cgagggatcg gactcactcg agacggatcg tataatttgg tcaagcgtgc
      601 cgagagatcg ctggaatgcc tcctcaccct cgagtcgagc gacccgattt ttgtcgagaa
      661 gtgcgtcagt acgacgaaga agatcgtcag tacgacggtt ggacacacaa aggacggccg
      721 tactgattgt gagggtgacc aaaagcgcga tcgtcgacgc gatcgtggcg gtgcgatgcc
      781 tgcgcgacca cttgactgcc cgatcgacta ccgtgggacg ccgggcgcgg atcggctcgt
      841 catcgaggaa tcgtctcagg tcgtctgcca acgcgcgtgc cgtgcgatac cgcgcggacg
      901 gctccttttc catggccttg aggatgatcg tttcgagatc cctgggtatc gaggggttga
      961 tccggcgcgg aggcaccgga tctccgtcga ggattcgccg caccagttcg tcgcgattgc
     1021 gcccactaaa cgccggacgg agcgtcagca actcgtagag cgtcacgccc agggcataca
     1081 aatccgttcg cgcatcgaca gacgcacggt cgcccctgac ttgctcgggg ctcatgtaac
     1141 ggagagtacc gacgaggtcg ccggtacgcg tcaggtcgtg ttcgtcctga gggaggcgag
     1201 ccagtccaaa gtcggcgatc cagagatggt cgcggacatc gatcaaaaga ttcgaaggct
     1261 tgatgtcgcg gtgaatgacc ccaatttcgt gagcgtgctc aagcgcaaat gcagcttgga
     1321 gcgcaagttg cgcggccgct ttgcagtgcc ttcgatcgcc tagcgacgat ccgggttgtc
     1381 tgggtgggaa tggcgaatgc aatgagcggc ggcgtgggcc aacgccaacc gttgtcgtag
     1441 gctcgacgcg gtcgtactgt ggcggcattt gcaacgcact gcgtatgcgt tcgttcaagg
     1501 attgcccgtc gatgaactgc atggcgtagt aatcgactcc ccggtcgcag ccgatcgcaa
     1561 agactggcac gatatgttcg tggtgcaaaa gggcggcggc ctgggcctca atccgaaagc
     1621 gctggcgatg gcgcgggtcg agcgacgcgg tcgacggtag gaccttgaga gcgacgcggc
     1681 ggcctagcgg cacctgttcc gcctcgtaga cgatacccat gccgccgcgg ccgacttccc
     1741 tgatgattcg gtattcaccc aacgttgccg aaagcagagt gtcctcagca ggatcgtgct
     1801 cggatcccgt gggagccaga tcgccagcgg cagcttgcgc aagctcaagg ccggcgaggg
     1861 gatcagccag gatatcggcg atcgaatggt ggagcgccag gaattccgcg cgggtgggcg
     1921 gcgacccgcc gcgcattagc tctccatagt cttcgagcgc cgccacaaca cgtcggtcat
     1981 tgatcgggga tgataccagg tcgacagttc tggaaggacc atttgccgac gccacactca
     2041 accgtgaatt catcgcataa cctcgcgaat cggacactct gaacgaccgc cggcgtgaaa
     2101 gtcccattca ggaaggtcac ccgagcccat tcattcgcgc cgcgataaat tctcgtggtc
     2161 atcgcacttc aagcacaacg atctcgttgt gggtgggatc atcggaccgc gatccgcgaa
     2221 tgccgacgat gcaatcgcca ggggcaatca gtcctttctc tcggcaccag tcgaggatga
     2281 acgcacggag ttcgtcggga ctggcgattt cgggcctggg cagcggtgta accccccaat
     2341 aaagcgccat tgctcgggct gtttgggggt catgggcaat tgcgagggtt ggggcggagt
     2401 tgcgttgctt tgaaagcacc aaggctgtac ggccagaaga cgttgcaact actaagagcg
     2461 ccgcgctgag ccgccgcgaa atcaagctgg cagcctcgac gacgctctcg gtaattggct
     2521 ggacacggcc agcacgcgcc accccgttgt tttgagtgga tccgtcgcga gctgcctccg
     2581 cgccgggcca gttggccacc gaccaagtcc agggagcgcc ggtgcgaaat tcggaaaata
     2641 gcagcttctc ggcctcgccg gcgattcgac tcatcatgga gacagattcg acgggatact
     2701 caccaatggc ggactcgccc gaaagcatca cggcatcggt gccatcgaga acggcgttaa
     2761 agacgtcgct ggcctcggcc cgagttgggc gactgctcga ttccatgctg ttgagcatct
     2821 gggtagccgt gatcaccggc acccgcgcgc gatggcatgc gccgatgatt tgcttctgga
     2881 tggccggcac cctgatcacg tccagctcga cgccgaggtc gccccgagca accatgacgg
     2941 cgtcggcctc ggcgacaatt gcatcgagat tggcaagtgc ctgcggcttt tcgatcttgg
     3001 cgatgatccg ggcgcggctg ccgcggcgct cgagctcgcg gcgtaagagc gcaatgtcat
     3061 cggccttacg cacgaatgag agcccgacat aatcgacctc gtgcgcggcg gtccagtcga
     3121 ggtcgaccag gtctttgtcc gtcagtgcac ccacgctcaa tccggcgcgc gggacattga
     3181 tgccttggtt cgagcgaatg tgcccgggca gcgtgacctt cagacgcgcc caacccgggc
     3241 ctcgagccac aacgtccatc gccaccgtgc catcggcaaa aagtacgctc tgccccacgt
     3301 ccaggtcgtc gggcaggctg cggtaagtcg aggtcaggtg atgcgcgtcg ctgccgtctc
     3361 ccggctcggc cgtcaagaca aactccgcat cttggtcgca aacgaccaca ccgccatgga
     3421 cttcgcctaa ccggatcttg gggccacaaa gatcctgcaa cacggcgagc tgcctctcgg
     3481 tctcatccgc gatccggcgg atcgcttcga gcgaggcagt atgcacctca tgagtcccat
     3541 gcgaaaagtt gagccgaaag acgtcaactc ccgcatcgac catctgcttc atgaccgccg
     3601 gattactcga cgccggaccg actgtcgcca caatcttggt ccgaacggct ccaagttttt
     3661 ctttgtgcat ggatttcact ttaacgtaat tacaaatgcg atcatgaaga ctgacgaact
     3721 tttgatcgcg caagtcgtgt cctacgctag gtagctcgat ttcccccaaa gcgcaatacc
     3781 gttagattag gtgtaactta ttagcgctag accctttctg tcgacctccc ccctggtggg
     3841 ggagggttgg ggtgggggga ggcaagtgcg agagcatccg ctcggccgcg gtccccgaga
     3901 ggaacgcccc ccaccctggc cctcccacac gaggagagag gggataacaa attactatgc
     3961 acctgtaagt tactgctaat tcaacggtat tgtcccaaag cggcgcaagt ctcaattctt
     4021 tcgtttttca gtctaataag ctgagacatt ctagtgaacg gggcggccca gggccagtca
     4081 ttgcatg
//
LOCUS       DATAQL010000420         7521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4927075, whole genome shotgun sequence.
ACCESSION   DATAQL010000420 DATAQL010000000
VERSION     DATAQL010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4927075"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="VHS97_07270"
     CDS             <1..1731
                     /locus_tag="VHS97_07270"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX3448038.1"
                     /translation="AIQRRVYQAGGPVLLFRRAKGTSFPLVANLFGTLDRAKFIFRDA
                     LEAVRRLVELKIDPGVFGKNPWRYRGTPRTLLRLLPRRVRSGPILAHRIAIDQLPQIQ
                     CWPVDGGPFVTLPQVYTEDPDRPGLARSNLGMYRVQLGGNQYVPNREVGLHYQIHRGI
                     GVHHAAAIQRGEALRVNVFVGGPPALSVAAVMPLPEGLPELAFAGALGGRRVAMVTPD
                     GSLPMPAEADFVICGSIDPRQRKPEGPFGDHLGYYSLQHDYPVLSVERVYHRDGAIWP
                     FTSVGRPPQEDTTFGALIHELTGPIIPTVVPGVQAVHAVDAAGVHPLLLAIARERYIP
                     DAPVRRPQEILTIANALLGQGQLSLAKYLLIVAQEDDPSLDIHDIGRFLRHLLERVDW
                     RTDLHFQTQTTIDTLDYSGHGLNQGSKVVIAAAGPLRRTLATELPDDLRLPSPFRDPR
                     VVLPGILAIEGQPYGDRSGDAIGFCKFFETGDSVRGFPLIVLVDDSAFAAKSERNFLW
                     VTFTRSNPAADIEGIGSFIDKKHWGCTGSLVIDARKKPHHAPPLVDDPKIERKVDELA
                     APGGPLHGVY"
     gene            1804..3018
                     /locus_tag="VHS97_07275"
     CDS             1804..3018
                     /locus_tag="VHS97_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HEX3448039.1"
                     /translation="MEPEDRKSDAKRRPVGASDLVESARKDPLDAGILVQQMVEGGKW
                     PAPELLERIVNAGNGAVGPLLDVLRAKPPSRPEPHVLRHVMGLLSVLRPPEAITELVH
                     LVEHGEEVASADAADALGQSGDAGFEALIKFCSSASFKGYRRSYVFDAAAFAALDNRA
                     RKSRLAEVLHPILEDTIAKAQEELKRVGWLKKNPPIDDLVDDDEFDEFDEDDDEVISD
                     IVGDEEHEIESEAYEDSDPDTTGDEEDIDDDLEEEFDVETSIAEEVAFVVGAMAIIAD
                     PAARDTIMTAFREGLVDESTTSKKRVDEHYAEAEEPIDEGEVEWNWLDSYRADYEEMG
                     HSAPPAHVPRTKYRYEDRYDEGEPPPDIPAIAPIRNTQPKLGRNDPCWCGSGKKFKKC
                     HLGKDGGSDRTG"
     gene            complement(3076..3411)
                     /locus_tag="VHS97_07280"
     CDS             complement(3076..3411)
                     /locus_tag="VHS97_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448040.1"
                     /translation="MTTKTSGWARTALRQQIEIFGEDQVSLDSLWRAVDSPNGHDPRR
                     WSVTATPLLAGFSAYLDRLQGNADRAADPSRLLWLWNGESKDPWHTGDLMSHEFIAWA
                     YATYLDSAL"
     gene            complement(3532..4722)
                     /locus_tag="VHS97_07285"
     CDS             complement(3532..4722)
                     /locus_tag="VHS97_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX3448041.1"
                     /translation="MTLISTEVESDLLRHSRPTIQQAMVPHGRRGQSGWKRTHKESTV
                     AIAVRHLSKRYGTFQAVDDVSFDVPAGQLVALLGPSGSGKSTILRIIAGLETAEQGSV
                     ELTGEDATAIPIQRRGVGFVFQHYALFRHMTIRDNIAFGLKVRKLPKAEIRARVDELL
                     NLVQLTGYATRYPSQLSGGQRQRVALARALAPRPKVLLLDEPFGALDAKVRDELRTWI
                     RRLHDEVHVTSLFVTHDQQEAFEVADQVVVLNRGRIEQMGPPQDLYEQPATPFVTEFL
                     GSVNVLRGQSNSGLGLVGGGLQVPTGLDENDGPISVYVRPHDLDLSHERNGGPGWPGR
                     VLRVTPLGAFVRLDVALADGTDVRVELSRERYAALNTRIGETLFVTPRDLKVFHDQSQ
                     PVGS"
     gene            complement(4719..5597)
                     /locus_tag="VHS97_07290"
     CDS             complement(4719..5597)
                     /locus_tag="VHS97_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HEX3448042.1"
                     /translation="MAIDVAASGYPGGTEAVAANRAQPIGRWLLIAAVLGWFALLILV
                     PSIALLRQVWAGGMRPFIETLTTPEVRRAFGLTLGITALATVVNTIFGIAIALVLVRQ
                     RFLGRALLDGVVDLPFAVSPVVAGLMLITLYGPEGWFGQWLERGGFRIIYAVPGMIVA
                     TLFVTLPFVVRELVPILRELGEEHEQAAYTLGAGRWQTFWRVTLPSIRWAVAYGVTLT
                     VARSLGEFGAVLVVSGNLIGQTQTATLYIHDGIESFNTQGAYAASLVLAAMSFVLLVG
                     MDLIRKRLDAREGKRP"
     gene            complement(5587..6420)
                     /gene="cysT"
                     /locus_tag="VHS97_07295"
     CDS             complement(5587..6420)
                     /gene="cysT"
                     /locus_tag="VHS97_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX3448043.1"
                     /translation="MASGVLKSGGGKSDLLLRATTLSYLGLMVVLPLAALTAQAARPG
                     ATAFLEALRDPYAWHALKLTFITALVMVFVNVLTGTATAWVLVRYEFPARGLVNALVD
                     LPFAVPTVVTGVMLVVLFGPASVVGTVLGRYGWGVIYHQPGIVLALLFVTYPFVIRSV
                     QPVLMELDRAEEEAAATLGAGAWTIFRRVTLPALWPSILTGGAVSFSRALGEFGSVVM
                     VAGNQPLATKTAPLYIFGEIESGNRHGALVISVALLASSLLILLVLNGVQRRWGAEHG
                     H"
     gene            complement(6427..>7521)
                     /locus_tag="VHS97_07300"
     CDS             complement(6427..>7521)
                     /locus_tag="VHS97_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3448044.1"
                     /translation="GRGMTECREGDVRMKHRVKTGTAFLLALWALLAPGCAPARDPNA
                     DLLKIGAYSVVREVLHDGLRPAFASEWKKRTGRAVTFDESYNASGAQARQIVSGLDVD
                     VAILSHEGDMELLVKAGRVKPTWKDGPHKGVVTHSLVVIGHRAGNPQSIKDWADLAKP
                     GVGVLYPDPKTSGGARWNINAIYGSAYLSSCDANSGKPDWAVIREFMARVQANVVNMD
                     QSGRQSMANFAERGTGDAVVTYENELLFRNKEHEPIPYVVPPATLLIESPAAVVATAV
                     ESHGSREVAEAFVEFLTSDAGQRIVADYGFRPVKPDAAAPSGALPLPPKLFTMADLGG
                     WAKIEEELYGPKGLWTSIFTANTSNRAAGR"
BASE COUNT         1560 a         2404 c         2202 g         1355 t
ORIGIN      
        1 gccatccagc ggcgagttta tcaggcgggg ggcccggtct tgttgtttcg cagggccaag
       61 gggacgtcat tcccgctggt ggccaacctg ttcggaacgc tcgaccgggc caagtttatc
      121 tttcgggacg cccttgaggc agtgcgccgc ctggtcgagc tcaagattga tccgggcgtt
      181 tttggcaaga acccatggcg ataccgcggc acaccacgca ctctgttgcg gcttcttccc
      241 cggcgcgtcc gctcggggcc gatcctcgct caccggattg cgatcgacca gttgccacag
      301 attcaatgtt ggccggtgga cggcggcccg ttcgtcaccc tcccccaggt ttacaccgag
      361 gatcctgacc gtcccggtct ggcgcggtcg aacctgggga tgtaccgggt tcagctcggg
      421 ggcaatcagt atgtgccaaa tcgagaggtt ggccttcatt atcaaattca ccgtggtatc
      481 ggcgtacacc acgcggcggc gatccagcgg ggcgaagcgc tgcgggtgaa tgtcttcgtg
      541 ggtggcccgc cagccttgtc ggtcgccgcc gtgatgccat tgcccgaggg actgcccgag
      601 ctggcttttg ccggcgcatt gggaggccga cgagtagcga tggtcacgcc cgatggctcc
      661 ctaccgatgc cggccgaggc agacttcgtc atctgcggct cgatcgatcc gcgccagcgc
      721 aagccggagg gtccgtttgg agatcatctc ggttattaca gcctgcagca cgactatccc
      781 gtgctctcgg tcgagcgtgt gtatcatcgc gacggagcca tctggccctt tacctccgtg
      841 ggacgtccac cgcaggaaga cacgacgttc ggcgccttga tccatgagct gaccgggccg
      901 atcattccca ccgtggtacc tggcgtgcaa gccgttcacg ccgtcgacgc cgccggggtt
      961 cacccgctgc tcctggcaat cgcccgcgag cgctatattc ccgacgcgcc cgtacgacgc
     1021 ccccaggaaa tcctcacgat cgccaacgca ctgctagggc aaggtcagct ttcgctggcc
     1081 aagtacctgc taatcgttgc ccaggaagac gacccttcgc tggatatcca cgacattggg
     1141 cggtttctcc gccacttgct cgagcgcgtc gactggcgca ccgaccttca cttccagact
     1201 cagacgacga tcgacaccct cgactattcg gggcatggac tgaatcaagg gtcaaaggtc
     1261 gtgatcgccg cggccggccc attgcggcgg accctcgcga cagaactgcc cgacgatttg
     1321 cggctgccgt cgccgtttcg agatccccgg gtcgttctgc ccggtattct cgccatcgag
     1381 ggacagccgt atggcgatcg gtcaggcgac gccatcgggt tctgcaagtt tttcgagacc
     1441 ggcgactccg tccgcggctt ccccttgatc gtgctggtgg acgacagcgc atttgctgcg
     1501 aaatccgaac gcaacttcct ttgggttacc ttcacccgat cgaatcccgc cgccgacatc
     1561 gagggaatcg ggtcgttcat cgacaagaag cactggggct gcaccggttc cttggtcatc
     1621 gacgcacgga agaagcctca tcacgcgcct ccgttggtcg acgatcccaa gatcgaacgg
     1681 aaagtcgacg agctggccgc gcccggcggt ccgttgcacg gagtctattg attacaccaa
     1741 acaccctgcc gagcgtagaa tcagaagtag tgtttatgga caaacccggg acagagattg
     1801 gccatggaac ccgaagaccg caaatcggac gcgaaacgta gacccgtcgg ggcaagtgat
     1861 cttgttgagt ctgcaaggaa agatccactc gacgcgggaa ttctcgtaca gcagatggtt
     1921 gaaggcggca aatggccagc tcccgagctt ctggaacgga tcgtgaacgc aggcaatggg
     1981 gccgtcgggc cgctccttga cgtgctgcgg gcaaaaccac cgtcacggcc cgagccccat
     2041 gtgctccgcc acgtcatggg cctcttgagt gttctacgtc cgcctgaggc gatcacggaa
     2101 ctcgtgcatc ttgtcgagca cggcgaagag gtcgcctcgg ccgatgccgc cgacgcgctt
     2161 gggcaatccg gtgacgccgg cttcgaggct ctcatcaaat tctgctcaag cgcatccttc
     2221 aagggctatc ggcggagcta cgtctttgac gccgccgcgt ttgcggctct tgacaataga
     2281 gcccgaaaat cccgactcgc ggaagtcctg cacccgattc tcgaagacac gatcgccaag
     2341 gcgcaggaag aactgaaacg ggtgggttgg cttaaaaaga acccgccaat cgacgatttg
     2401 gtggacgacg acgaattcga tgaattcgac gaggacgatg acgaagtaat ctcggacatc
     2461 gtgggcgacg aggaacatga gattgagtcc gaagcgtacg aagattccga ccccgacacg
     2521 accggtgacg aagaagacat tgatgatgac ttggaagagg agttcgacgt cgagacgtct
     2581 atcgccgagg aagttgcgtt cgtggtcggc gcgatggcga tcatcgccga cccggcggcc
     2641 cgcgacacaa tcatgactgc gtttcgcgaa ggattggttg atgagtcgac tactagcaaa
     2701 aagagagtcg atgagcacta tgccgaggct gaagaaccta tcgacgaagg agaagtcgaa
     2761 tggaattggc ttgattctta tcgggcggat tacgaggaga tggggcactc agcgccgcct
     2821 gcgcatgtgc cgcgtactaa atatcgatat gaagatcgat atgacgaagg cgagccgccg
     2881 ccggatatcc ccgcgatcgc tcccatccgt aacacgcagc cgaaactggg acgcaatgat
     2941 ccgtgttggt gcggtagtgg caagaagttc aaaaagtgcc atctggggaa agacggcggg
     3001 agcgatcgaa ctggatgatc gaggcattca cgcgatgatt gacacgggcg gctgggatcg
     3061 cgccccaact cactctcaga gcgcagaatc gagatacgtc gcgtaggccc aggcgataaa
     3121 ctcatggctc atcagatcgc cggtgtgcca ggggtctttc gattcgccgt tccagagcca
     3181 caacagccgc gaaggatcgg ccgccctgtc cgcgttgcct tggagccggt caagataggc
     3241 ggaaaatccg gctaagagcg gtgtcgcggt gaccgaccaa cgtcggggat cgtgcccgtt
     3301 cggggaatcg acggctcgcc agagactgtc gaggctgacc tgatcttcgc cgaagatttc
     3361 aatctgctgc cgcagcgccg ttctggccca gccgcttgtt tttgtagtca tgacttgctc
     3421 ctcgcgaacg acgacttcct gttatcggtt ccgtggttaa gagccggggc aagctcggca
     3481 cgtctcgagc ctgtcctttt cgacatttcc gcgcctctcg gacaggcgcg gtcatgatcc
     3541 aacaggctga gactgatcgt gaaagacctt gaggtctctt ggggtcacaa acagcgtttc
     3601 gccgatacgg gtgttgagcg cggcgtaccg ctcgcgggaa agttcgaccc gaacgtcggt
     3661 gccgtcggcc agtgcaacgt cgagccgcac gaaagcaccc agtggggtca ctcgcaatac
     3721 ccggcctggc caaccaggcc cgccgttacg ctcgtgactc aagtcgaggt cgtgaggacg
     3781 cacgtagacc gaaatcggac catcgttctc atcaaggccg gtcggcacct gcaatcctcc
     3841 gccgactaaa cccaagccgg aattgctctg gcccctcaag acgttgacgg atcccaagaa
     3901 ctcagtcaca aacggcgtgg ccggctgttc gtacagatct tgcggcgggc ccatctgctc
     3961 gatgcggccc cgattgagta cgaccacctg gtcggcgacc tcgaacgcct cttgctgatc
     4021 gtgcgtcacg aacaggctgg tcacgtgaac ctcatcatga agtcggcgga tccaggtcct
     4081 taactcgtcg cgaaccttcg cgtcgagggc tccgaatggc tcgtccaaga gcagaacctt
     4141 gggccgcggt gccagagccc gcgccaatgc cactcgctga cgctggcccc ctgaaagctg
     4201 cgacgggtag cgcgtggcat atcccgtgag ctggaccagg ttcaagagct catccacgcg
     4261 cgcacggatc tcggccttgg gcagtttacg aaccttgagg ccgaacgcga tattatcccg
     4321 gatggtcata tggcggaaga gcgcataatg ctgaaagaca aaccctactc cgcggcgctg
     4381 gatcgggatc gccgtggcgt cttcgccggt gagctcgacc gagccctgtt cggcggtctc
     4441 caggcccgca atgatccgca gaattgtgct cttgcccgaa cccgacggcc caagcaaagc
     4501 cacaagctga ccggcgggca cgtcgaatga aacgtcgtcc accgcctgga atgtaccgta
     4561 ccgcttggaa agatgacgca ctgcgatggc cacggttgac tccttgtgag tgcgtttcca
     4621 gccgctctga ccacgccggc catgaggcac catggcctgc tggatcgtcg gtcgagaatg
     4681 tcggagaagg tcgctttcga cctctgtaga gatcaaggtc atggccgctt tccctcgcgt
     4741 gcatccagcc gtttacgaat cagatccatg cccaccagca agacaaacga catcgcggcc
     4801 aacaccaggc tcgcagcata ggctccctga gtgttaaagc tctcgatccc gtcgtggatg
     4861 tacaaggttg cggtctgggt ctgcccgatc aggttgccgg agaccacgag caccgcgcca
     4921 aactcgccca gcgacctggc aacggtcagc gtcacgccgt acgccacggc ccagcgaatt
     4981 gagggcaggg tcaccctcca aaacgtctgc cagcgcccgg ccccgagcgt atacgctgct
     5041 tgctcatgct cctcccccag ctcgcgcaga atcggcacca gctcgcggac gacaaagggg
     5101 agcgtcacaa acagggtcgc cacgatcatg ccggggaccg catagatgat ccgaaaccca
     5161 ccccgctcga gccactggcc aaaccagcct tccggcccgt aaagcgtgat cagcatcagc
     5221 ccggccacaa ccggcgagac agcaaatggc aagtcgacga caccgtccag cagagctcgc
     5281 cccaggaaac gctgccgtac caagaccaat gctatggcga ttccgaagat cgtattgacc
     5341 accgtcgcca gcgcagtgat acccagcgtc agcccgaacg cacggcgaac ttcgggcgtc
     5401 gtcagtgtct cgataaaagg ccgcatgcca ccagcccaga cctggcgcaa gagggcgatg
     5461 ctcggcacca ggatcagcaa ggcaaaccaa ccgagcaccg cggcgattaa gagccaacgg
     5521 ccgatcggct gggctcgatt cgctgcaaca gcctccgtcc cgcccggata tccactcgcc
     5581 gcgacgtcaa tggccatgct cagcccccca acgccgctgg accccgttga gcacgagtaa
     5641 tatcagcagc gacgacgcca gcaatgccac cgagatgacc aaggcgccgt ggcgattgcc
     5701 gctttcgatt tcgccgaaga tataaagcgg ggcggtcttg gtcgccaacg gctgattacc
     5761 ggcaaccatc accacgctgc caaactcccc cagggcccgc gaaaaggaca ctgccccccc
     5821 ggtcaggatc gatggccaga gcgcgggcaa tgtgacccgg cgaaagatcg tccacgcacc
     5881 cgcccccagt gtcgcggcgg cttcctcctc ggcgcgatcc agctccatga gtaccggctg
     5941 cacgctccgg atcacaaagg ggtatgtcac gaacaagagc gctagcacga ttccaggctg
     6001 atggtagatc accccccagc cgtaacgccc caagacagtc cccactacgc tcgccggacc
     6061 aaaaagcaca acaagcatca cgccagtgac gaccgtgggt acggcgaacg gcaggtcgac
     6121 cagtgcgttg actagccccc tcgccggaaa ctcgtaccgg accaagaccc acgccgtcgc
     6181 cgtgccggtc agcacattca cgaacaccat cacaagcgcc gtgatgaacg tcagtttcag
     6241 agcgtgccag gcgtatgggt cgcggagcgc ctccagaaac gcagtagcgc ccggacgagc
     6301 cgcttgggcg gtcagtgccg cgagcggaag aaccaccatc agcccgaggt aactcagcgt
     6361 cgtggcccgc aaaagcaggt ccgacttacc gccgccggat ttgagcactc ccgaagccat
     6421 aggtcgttac ctccccgcag ctcggttact cgtgttggcg gtaaaaatcg aagtccagag
     6481 ccccttcggc ccgtaaagct cttcctcgat cttggcccaa ccccccaggt cagccatcgt
     6541 gaaaagcttc ggcggcaatg gcagtgcgcc actgggtgcc gcagcatccg gcttcaccgg
     6601 tctaaagccg tagtccgcca cgatccgctg cccggcgtcg gacgtcagga attcgacaaa
     6661 agcctcggcc acttctcggc tgccgtgcga ctcgacggcc gtcgcgacca ccgccgccgg
     6721 gctctcgatc aaaagagtcg cgggaggaac cacatacgga atcggctcat gttccttgtt
     6781 gcggaataac agctcgttct cgtaggtgac cactgcatca ccggtgccgc gctcagcgaa
     6841 attggccatg ctctgtcggc ccgactgatc catgttgacc acatttgcct ggactcttgc
     6901 catgaactcg cgaatgacgg cccagtccgg cttgccgctg ttggcgtcac acgacgacag
     6961 ataggccgac ccgtaaatgg cgttgatatt ccagcgagca ccaccggacg tcttggggtc
     7021 gggatagagc acgcccacac ccggcttcgc cagatcggcc caatccttga tcgactgggg
     7081 atttcccgca cgatggccga ttaccaccaa actatgggtt accacgcctt tatggggccc
     7141 atccttccag gttggtttca cgcgaccggc cttgaccagg agctccatat cgccttcgtg
     7201 agaaagaatc gcaacatcga cgtctaaccc cgaaacgatc tgcctcgcct gagcgcccga
     7261 agcgttgtat gattcgtcga aggtgacagc gcggccggta cgcttcttcc actcactggc
     7321 aaaggccggc ctcagcccat catgcaacac ctcgcggacg actgagtaag ctccaatttt
     7381 gagcaaatca gcattgggat cgcgcgcggg agcacagcca ggagccagta aagcccacag
     7441 cgccagtaga aacgcagtcc cggttttgac cctgtgcttc atcctgacat ctccttcgcg
     7501 gcattccgtc atgccgcgac c
//
LOCUS       DATAQL010000421         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3399439, whole genome shotgun sequence.
ACCESSION   DATAQL010000421 DATAQL010000000
VERSION     DATAQL010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3399439"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VHS97_07305"
     CDS             complement(<1..1038)
                     /locus_tag="VHS97_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX3448045.1"
                     /translation="MSLIHPVLRAASPSLTAIRPVGGQRGTQLEITLSGARLGDAQEI
                     IYYHPGISTVGLKKLDDNSVRATLKIAPDCALGLHDLRVTTATGISELRTFSVGALKE
                     MSEVEPNNDFAAPQAIPMNVTVNGVADNEDVDYYVVEAKKGDRISAEVEGLRLGISEF
                     DPYVAILDSKRFELGVSDDAALAWRDGFTSIIAPHDGKYIVQVRESAYAGNASCLYRL
                     HLGNFPRATAVVPAGGKTGETISVRWIGDPTGETTTAIKLPATIERDFGIARSDDKGI
                     SPYPNTFRLTSLGNVIENEPNDDQAHATPFEAPMALNGVIEKAGDVDHFVFKATKGQV
                     LDIHCYARRIRS"
     gene            complement(1120..2430)
                     /locus_tag="VHS97_07310"
     CDS             complement(1120..2430)
                     /locus_tag="VHS97_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3448046.1"
                     /translation="MANGRIQNRMNRRGFLTVGSIGLGGLTLADVIRLEARADLKSYA
                     AITAKADSVIHIFLPGGIAHQETFDPKPFAPVEYRGDMGSIATKLEGEKFSQTLPKTA
                     QVADKLTVIQSMTHGEAAHERGTHNMFTGYRPSPALQYPSMGSVVSHEFGPKNNLPPY
                     VCIPRQPNVYAGTGYLSSSFSPFSLGRDPANPKFKVQDLNLPGDISEARFTTRRNVLD
                     AVNGYFAAREKSDNIAAMDTFYERAYSLISSPKAREAFNIAAEPANIRDDYGRNAAGQ
                     RLLMARRLVAAGVRFVTVQYGSWDLHYQIVAGMNSQMPAFDQAFAALIRDLDRTGLLN
                     RTLVMVSSEFGRTPKINKDAGRDHWPKVFSVVLAGGGVKKGAIYGSSNATATEPERDP
                     IGPEDLATTVYHLLGIVADKELMAPGNRPIEIVDGGKVVKDLLA"
BASE COUNT          547 a          805 c          827 g          563 t
ORIGIN      
        1 cgaacgaatc cggcgggcat agcaatggat gtccaatacc tggcctttgg tggccttgaa
       61 gacaaaatgg tcgacgtcac ccgccttttc gatcacgccg ttcaatgcca tcggtgcctc
      121 gaacggcgtc gcatgcgcct gatcgtcgtt gggctcattc tcgatcacgt ttcccagcga
      181 agtgagccgg aacgtgttgg gataggggga aatccccttg tcatccgacc gcgcgattcc
      241 gaaatcccgc tcgatggtgg cggggagctt gatggccgta gtcgtctcgc cggtgggatc
      301 gccgatccag cgcacggaaa tggtttcacc cgttttgccg ccagccggta cgaccgcggt
      361 cgcccgcgga aaattcccca gatgcaaacg ataaagacaa ctggcgttgc ccgcataagc
      421 actttcgcgt acctggacga tgtacttgcc gtcgtgggga gcgatgatcg acgtgaagcc
      481 gtcgcgccag gcgagcgccg cgtcgtcgct gacaccgagc tcaaaccgtt tggagtcgag
      541 gatcgccacg taggggtcga actcactgat gcccagacgc aacccctcaa cctcggccga
      601 aatccggtct ccctttttgg cctcgaccac gtagtaatcg acgtcttcat tgtcagccac
      661 gccgttgacc gtcacattca tggggatcgc ctgaggagcc gcgaaatcat tgttgggctc
      721 gacctcgctc atttccttga gcgcgcccac gctgaaggtg cgaagttcgc tgataccggt
      781 cgccgtcgtt actctgaggt cgtgaagccc cagcgcgcaa tccggcgcga tcttaagtgt
      841 ggcgcgaacg ctgttgtcat cgagcttctt gagaccgacc gtggagattc ccgggtggta
      901 gtagataatc tcttgagcgt cccccaggcg ggcacccgag agcgtgatct ccaactgcgt
      961 gccccgctgg ccgccgacgg gccgaatcgc cgtcaacgag gggctggccg ctcgaagaac
     1021 ggggtggatc aacgacatga gggcaagcgt tgcaacgcat cggcgcatac gaaagtctcg
     1081 aatgaagcgg tagatgaaaa tagttgtcaa gatgctacgt caagcgagta gatccttgac
     1141 gaccttaccg ccgtcgacga tctcgatcgg ccggttgccg ggagccatca attccttgtc
     1201 ggccacgatg cccagcaggt ggtaaaccgt agtggccagg tcttccggac cgatgggatc
     1261 gcgctcgggc tcggtggccg tggcgttcga cgagccgtag atcgcaccct tcttgacgcc
     1321 gccgccggcc aggacgacgc taaagacctt gggccagtga tcgcggccgg cgtctttgtt
     1381 gatcttggga gtgcggccga attcgctcga caccatcacc aaagtccggt tcaacaagcc
     1441 cgtccggtcc aggtcgcgaa taagagcggc gaaggcctgg tcgaaggcgg gcatctggct
     1501 gttcatcccg gcgacgatct ggtaatgcaa gtcccagctg ccatattgga cagtgacgaa
     1561 ccgaactccg gcagcaacca gccggcgcgc catcagcaac cgttggccgg ctgcgttccg
     1621 gccatagtcg tcgcgaatgt tggcgggctc ggcggcgatg ttgaacgctt cgcgggcctt
     1681 cggagagctg atcagactgt aggctcgttc gtagaatgta tccatcgccg cgatgttgtc
     1741 gctcttttca cgggcggcaa agtagccatt gaccgcgtca agcacgttcc gcctggtcgt
     1801 gaatcgcgcc tcgctgatgt caccgggcaa attcagatct tgcaccttga atttcgggtt
     1861 cgccgggtcc cgacccaggc tgaacggaga aaaagacgag ctcagatagc cggtgccggc
     1921 atacacattg ggctgcctgg gaatgcatac gtacgggggc aggttattct tgggcccaaa
     1981 ttcgtgcgag acgacgctcc ccatgctcgg atattggagg gctgggctgg gacggtatcc
     2041 cgtgaacata ttatgcgtgc cgcgctcgtg agccgcctca ccgtgcgtca tcgactggat
     2101 cacagtgagc ttatcggcga cctgggcggt cttgggtagc gtctgactga acttctcacc
     2161 ttcgagcttg gttgcgatcg aacccatgtc accgcgatat tcgacaggag cgaacggctt
     2221 gggatcgaac gtttcctggt gcgcgatccc gccgggcaag aagatatgga tcaccgagtc
     2281 ggccttggct gtgatcgccg cgtaactctt gagatccgcc cgggcctcga gccggatcac
     2341 gtcagccaga gtcagtcccc ccagtccgat cgatccaacc gtaagaaaac ctcgacgatt
     2401 cattcggttc tggatgcggc cattagccat cgacgtaact ccttgatcgc agtgaagtca
     2461 taaatctttg gtgcggtagc aattaggcgg gagtttttgg atcgtcgccg actgcggagg
     2521 actcagaacc aaaggcagcg tcgctaagca cttgttgaat aatacgggtc aactgcggtc
     2581 ggcgctggcg ctaatttcta aaaagattcg ccattgaaac cgcgggagcc acagacgatc
     2641 ggagtgctct cgcgcgccac gaaggaccac ggagtcgcta tttcaactcc gcgcaggttt
     2701 ggaaagccgt tgaccaacct gataccatcg gcttacgccc tt
//
LOCUS       DATAQL010000422         4605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5233436, whole genome shotgun sequence.
ACCESSION   DATAQL010000422 DATAQL010000000
VERSION     DATAQL010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5233436"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VHS97_07315"
     CDS             <1..562
                     /locus_tag="VHS97_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448047.1"
                     /translation="KRREGTLAWRWTAQRLAISAFATFHLSALIIWTIPPCAIKERFQ
                     GPYRYYMLPLGIWQWWAIFAPNPVRENQILEAEVIDAKGIRHIHEFTKIGDMSSWDKM
                     FRYRDPKFTGNMMNDEYSKQRVLTARHAVRQLGLKENAFPLWVSLYVKVSDTPPAGTT
                     DVDLMSPPRIQVLDRFEFASLKEVRP"
     gene            559..1575
                     /locus_tag="VHS97_07320"
     CDS             559..1575
                     /locus_tag="VHS97_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX3448048.1"
                     /translation="MNPLKDWNCFFFRPISARPLGAFRIVFGIIILIYLGIMSVEFDF
                     WYTGAGLLQGNEAREAAGPLRFSPLQYVTDPTVAHLFYAFTVAVAFGFTLGWRTRLMS
                     ILLYLCMISLYHRNVSSNGGPDAVPTILTFYMMFCPSGAAYSLDALRAARKRGSAAEP
                     LIVPWSVRLLQMQLCLIYFQSTLIKLQGNLWLNGTTVHYILFLREYRDQNMEWLAQYP
                     LLINMMTHGAILIQFTLAFWLWFKPTRKWAILGGVMLHLGIRPILNIPGFGEVMTAAY
                     LTFLAPDELDALLRFLDPRAWLARLGLGFQARALWSSRGRPTAVPGYQQLELPFEGLD
                     GQAV"
     gene            complement(1920..2438)
                     /locus_tag="VHS97_07325"
     CDS             complement(1920..2438)
                     /locus_tag="VHS97_07325"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3448049.1"
                     /translation="MPNAQEIIAALGLKPHPIEGGFFRETYRALGMVPAGSLPPAYSS
                     QADRSLGTAIYYLLTAETFSEMHRLPTEEVFHLYLGGPVRMLQLFPGGAARQVILGTD
                     ILAGQNPQVVVPSEVWQGSRLEPGVDFALLGATMAPGFEYADYEQGRRSELTALYPDL
                     ADLIRQLTRNDL"
     gene            complement(2534..3661)
                     /locus_tag="VHS97_07330"
     CDS             complement(2534..3661)
                     /locus_tag="VHS97_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3448050.1"
                     /translation="MSDLKITGVEAIYLRLPEVKTQCDSGQDALIVKVTTDAGITGYG
                     EVDSNPMAVKGCIEGPFSHTTATGLAHAVIGEDPFQTEYLWHKMYRANIYGGRRGIGV
                     HAISGIDLALWDIKGKALGLPVWKLLGGGFRKSLRAYASSLFGATPAETGDRARRFAD
                     QGFTAVKFGWDPMGQDRDTDVALVREARAGLGPTIDLMIDAGLVYDAKTAIQRARAFE
                     QFDPFWFEEPLLPDDYEGYAKLSASTSLRIAAGEEESERKSFLQLMDVGKIDVVQVDL
                     TRCGGFTEAIKIAALAADRGLPIVNHGFTTYLNVAAALHFLASIPNTLGLLEFVVEEG
                     TNLRQSITETVKARDGMVAVPDAPGLGLEMLEDGIRKFRVA"
     gene            3939..>4605
                     /locus_tag="VHS97_07335"
     CDS             3939..>4605
                     /locus_tag="VHS97_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448051.1"
                     /translation="MFRALLGWSLFVLLCCGTRVAVAQDPPPLEAPTLEPDKPKASAP
                     EPPKPAQPPAGAVSAAPKPATPSSPSPVRPMLVIPGVTAPGQRPGSTGRFKAAPPSGP
                     ANSPGATSDNSVRSLASPPALGSPFRPTVPAPATPVPGASSLSSIPLTIEPLENVPSR
                     NQAPAGSSRPHVDSAGPPGAITADESGWAGASTKPAARRLPGVLGRIMNQSPATPPRD
                     PTRG"
BASE COUNT          867 a         1445 c         1341 g          952 t
ORIGIN      
        1 gaaacgaagg gagggaacct tggcctggcg atggaccgcc caacggctgg cgatttccgc
       61 ctttgccact tttcatcttt cggccctgat catctggacc attcccccgt gcgcgatcaa
      121 ggagcgattc cagggcccct acaggtacta catgttaccg ctggggatct ggcaatggtg
      181 ggcaatcttc gcgccgaacc cggttcgcga aaatcagata ctggaagccg aggtcatcga
      241 cgccaaaggg attcgccaca ttcacgagtt caccaagatc ggtgatatgt cgtcgtggga
      301 taagatgttt cgatatcgcg atcccaagtt caccggcaac atgatgaatg acgaatactc
      361 caagcagcga gtgttgacgg cgcgccatgc ggtccgtcaa ttgggattga aagaaaacgc
      421 gtttccgctc tgggtgagtc tttacgtcaa ggtgtcggat actccacccg ccggtacgac
      481 cgacgtcgat ctgatgagcc cacctcgaat ccaggtgctg gatcgattcg aattcgcctc
      541 gctcaaggag gtgcgtccgt gaacccatta aaggattgga attgcttctt tttccgaccg
      601 atctcggcac ggccgttagg ggcgttccgg atcgtgttcg gcataataat cctgatttat
      661 cttggcatca tgagcgtcga gttcgacttc tggtacaccg gcgccggctt gctgcaagga
      721 aatgaagcca gggaggctgc cggtccgttg cgattctcgc cgctccaata tgtgactgat
      781 ccgacagtgg ctcatctttt ttatgcgttc acggtcgcgg tcgcgttcgg cttcaccctg
      841 ggctggcgca cccggctcat gagcattctg ctttatcttt gcatgatctc gctctatcac
      901 cgcaatgtct catccaacgg cggacccgac gccgtgccga cgatcctgac gttttacatg
      961 atgttttgcc cgagcggggc ggcctattcg ctcgacgcct tgagggccgc caggaaacga
     1021 gggtccgcgg cagagccgct catcgtcccc tggtccgtcc ggttgctgca aatgcagctt
     1081 tgcttgattt atttccaatc gacgctcata aagctgcagg gcaacctctg gctcaatggc
     1141 accaccgtcc attacatcct cttcctccgt gaataccgcg atcagaacat ggaatggctg
     1201 gcgcaatacc cgctcttgat caacatgatg acgcatggcg ccattctgat ccaatttacc
     1261 ctggccttct ggctctggtt caagccaacg cgcaagtggg caattctggg aggcgttatg
     1321 ctccacttgg gcatcaggcc catcctcaac atccccggct tcggcgaggt catgaccgcc
     1381 gcctacttga cctttcttgc tcccgatgag cttgacgccc tgctgcgatt cctcgacccg
     1441 cgcgcctggt tggcgcggct tggcctgggc tttcaggcca gagcgctgtg gagctctaga
     1501 ggacgaccaa cggcggttcc gggataccag cagctcgagc taccgtttga agggctcgac
     1561 ggccaagcgg tttgagcagg aaaatcgagc ccggtactca aaaaccgcgc gccagggcgg
     1621 cagcgaccgg gtgcttcaac agtttccggc atccttgtcg tgccctttca gggcactgcg
     1681 catggtttac gcctggacaa cccagggcgt tgccctgggc tacacccagg gcgttgcact
     1741 gggctacacc agggcgttgc cctgggctac accagggcgt tgccctgggc tacacccagg
     1801 gcgttgccct gggctacttt gttgatgccc ctttggggca gagatttcgg ggtttatccc
     1861 cctcaatttc ggcccggagg gccattcaca tgccagccca gggcaacgcc ctggggcaat
     1921 cacagatcgt tgcgggtcag ttgccgaatc aggtcggcga ggtcgggata aagggccgtg
     1981 agttcagaac gccggccctg ttcgtagtcg gcatattcga atccaggggc catcgtcgca
     2041 cccaaaaggg cgaagtcgac tcctggctcc agccgcgaac cttgccatac ttccgacgga
     2101 acgacgacct gtgggttctg gccagccagg atgtcagtgc cgagaatgac ctggcgcgcc
     2161 gcgcctcccg gaaaaagttg aagcatccgg accggccctc ccagatagag atgaaaaacc
     2221 tcctcggtcg gtagtctgtg catctcagag aaagtttcgg ctgtcagcag atagtaaatg
     2281 gcggtcccca gagaccgatc tgcctggctg gaataagccg ggggaagcga cccggctggt
     2341 accatgccta gagcccgata ggtttcccgg aaaaatcctc cttcaatcgg gtgcggcttc
     2401 agacctaacg cggcgatgat ctcttgggca ttgggcatgg cgatggatcc acgattttcg
     2461 cgtccgattg atcttcgttg gcacacgtgt caaaacctgg aggcgatgcg gacgaaggca
     2521 gaaccaatcc ggctcaagcc acacggaatt tccggatccc gtcttctagc atttcgagac
     2581 ccaatcccgg ggcgtcgggc accgcgacca ttccgtcgcg ggctttgacg gtctcggtga
     2641 tcgattgccg cagattggtc ccttcttcga cgacgaactc gagcaaaccg agtgtattgg
     2701 gtatgcttgc caggaaatgg agcgcggcgg cgacgttcaa gtaagtcgtg aatccgtggt
     2761 taacgatcgg cagtccccga tcggccgcca gggcggcgat cttgatcgcc tcggtaaagc
     2821 cgccgcaacg ggtgagatcg acttggacga cgtcgatctt ccccacgtcc atgagctgca
     2881 gaaacgattt tcgctcactc tcttcctcgc cggcggcgat ccgcaaactg gtactggcgg
     2941 acaacttggc atagccctcg taatcgtcgg gcaagagcgg ctcctcaaac cagaaggggt
     3001 cgaattgctc aaaggctcgc gcgcgctgaa tggcggtctt ggcatcgtag acgaggccgg
     3061 cgtcgatcat caaatcgatc gtgggtccca ggccggcgcg agcctcgcgc accagggcga
     3121 cgtcggtgtc gcgatcctgg cccatcgggt cccagccgaa cttgacggcc gtgaacccct
     3181 ggtcggcaaa ccgccgcgcc cgatcgccgg tctcggcggg ggtcgctccg aacagcgaac
     3241 tggcatacgc ccgcagcgat ttccggaaac cgccgccgag cagtttccac acgggcaggc
     3301 cgagcgcctt ccccttgata tcccagagcg ccagatcgat gccggagatc gcatggacgc
     3361 caatcccgcg ccgaccgcca taaatattgg cccggtacat cttgtgccag agatattcgg
     3421 tctgaaacgg gtcttcgccg atcacggcgt gtgccaaacc ggttgccgtc gtatgcgaaa
     3481 acggcccctc aatgcatccc ttcacggcca tcgggttgga gtcgacctca ccgtaacccg
     3541 tgattccggc atccgtggta accttgacga tcaaggcgtc ctgaccgctg tcgcactgtg
     3601 tctttacctc gggcaagcgc aggtagatcg cttcaacccc ggtgatcttg aggtcgctca
     3661 tcgcttagtt tgcccctagt tacgaaggct tgtgcacgac caatcatcgc tgaaaaacat
     3721 catttacgtc ttctcgcggt caggcggatc gcggcgaact cgctgcattt tacaccggcg
     3781 caccccgaat tcgagagcct gacccttggt tgtcgtatcg catggggaag tacgggctgc
     3841 tcctaaaaaa acggattatt ccggcccgcg cggaccgatg ataccagggt aagcgcttcg
     3901 ctttccgtag gtcgtccttt aatcgagaca cgagcccgat gtttcgagca ctcctggggt
     3961 ggtcgttatt tgttttgctc tgctgcggca cccgtgtggc cgtggcccag gatccgccgc
     4021 cgctggaagc acccaccttg gaaccagaca aacccaaggc gagtgcgccc gagcctccga
     4081 agccagcgca gccgccggcc ggcgcagttt ccgcagctcc caaacctgcc acgccttcct
     4141 caccatcgcc ggtcaggccc atgctggtga ttcccggcgt gaccgcgcca ggtcagcgcc
     4201 ctggatcgac aggcaggttc aaggcggcac cgccatcggg gccggcgaat tcaccgggcg
     4261 cgacgagcga taattccgtt cgctcgctgg cgagcccgcc agctttgggt tctccctttc
     4321 gcccgacggt tccggcgccc gcgacacctg ttcccggggc gtcgtcgcta tcgtcgattc
     4381 cgctcacgat cgagccgctc gagaacgttc catcgcggaa tcaagccccc gccggctcgt
     4441 ctcggccgca cgtcgattcc gccggtccgc ctggagccat tactgcggat gagtccggct
     4501 gggctggggc cagcaccaaa ccggccgcta ggcgtttgcc gggcgtgctt ggccgcatca
     4561 tgaatcaatc ccccgccacc ccgccgcgcg atccgacgcg tggcg
//
LOCUS       DATAQL010000423         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1874713, whole genome shotgun sequence.
ACCESSION   DATAQL010000423 DATAQL010000000
VERSION     DATAQL010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874713"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VHS97_07340"
     CDS             complement(<1..246)
                     /locus_tag="VHS97_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3448052.1"
                     /translation="MSNFSDSGSTHHFGKVYLVGAGPGDPGLLTQKGASVLARADVVV
                     YDHLASARLLDLAPPEAQRVCAGKSIGHCILSQEEINE"
     gene            complement(313..1338)
                     /gene="tatC"
                     /locus_tag="VHS97_07345"
     CDS             complement(313..1338)
                     /gene="tatC"
                     /locus_tag="VHS97_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX3448053.1"
                     /translation="MPKDQDLFSEEQSMVTMSFGEHIEELRVRLILGLIGLFVGVVIV
                     FIPPLDIGWRVMKKMEEPAKVALAAFYRAEYDKKAKKADEDKAISKPVQAVFEAGDLV
                     SELKKVAPKLELPDIETLKGKSLVLPMQFFAAGLIKAVEGSTVQIDQSLVSLAPLETL
                     TIFFMVCLVSGLVLASPWVFYQAWAFVAAGLYRHERGYVKKYLPFSLGLFLAGVFLCF
                     LFVLPITLNFLLQFNVWLGVAPTLRLSEWMSFATVLPLIFGLSFQTPLVMLFLERINV
                     FTVDDFRAKRKMAILVITIAAAVLTPGQDPFSMLLLAIPMIFLYELGIILIGRNQRKE
                     TYAGWSS"
     gene            complement(1546..2499)
                     /locus_tag="VHS97_07350"
     CDS             complement(1546..2499)
                     /locus_tag="VHS97_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3448054.1"
                     /translation="MTQSDLVPADEPFQSVPACRRVRRAAKLIGAIGLAVTGCVLAYH
                     SLWSFRDEWASLTANGPQFQREMAAEIAEYTLVRGVPMYEAHCQRCHGADGHGDGPAL
                     TDVLQRPRDLASASWRAGTDRAAVRRVIEDGTADKAMHGLAGVLTTGELDSIVDYVLS
                     LEISALVKKTGLSPGGGAIAPPVKFRDADGITGTLEQHRGKFVVVAFWGTTCVPCRKE
                     LPELALLAKKFENAGLVVLPVCLDETSAKTASEVAASIAPDLHVYVDHDGSIPKAYKI
                     HHMPQTFVIDREGRMLAWSIGFANWSGKQIEELFRSFGVRQ"
BASE COUNT          609 a          846 c          789 g          529 t
ORIGIN      
        1 ttcgttgatt tcttcctggc tcaagatgca atgaccgatc gacttgccgg cgcaaacacg
       61 ctgggcctcc ggcggggcaa gatccaatag ccgcgcactg gcgagatggt cgtataccac
      121 cacgtcggcc cgcgccagga cacttgcccc cttctgcgtc agaagccccg gatcacccgg
      181 cccggcaccg accagataga ctttcccgaa atgatgcgtg cttcccgagt ccgagaagtt
      241 cgacaaagga acagatgctc acagagcacc ctccgtcagt cttcaacaca cgtcttgctt
      301 cacacgggat gactaactcg accaacctgc gtatgtctct ttgcgctgat tgcggccaat
      361 caagatgatg ccgagctcgt agagaaagat catcggtatg gccaagagca gcatgctgaa
      421 cggatcttgc cccggcgtca gcaccgccgc ggcgatcgtg atgaccagga tagccatctt
      481 ccgtttggct cggaaatcat cgactgtgaa aacattgatc cgctccagga acagcatgac
      541 caacggggtc tgaaacgaga gaccgaagat cagcggcaag accgtggcga aactcatcca
      601 ttcgctgagt cgaagggtcg gagcaacgcc cagccatacg ttaaactgaa gcagaaaatt
      661 gagcgtgatg ggtaaaacga acaggaagca aaggaacacg cctgccagga acaagcccag
      721 cgaaaacggc aggtatttct tcacgtaccc ccgctcatgc cggtatagcc cagcggcgac
      781 aaacgcccag gcttgataga acacccaggg acttgccaga acgagtccac tgaccaggca
      841 caccataaaa aagatggtga gagtctccag gggcgcgagc gataccaggc tctggtcgat
      901 ctggaccgtg gaaccctcaa cggccttgat gagccccgcc gcgaaaaact gcatcggcaa
      961 caccagcgat ttgcctttga gtgtttcgat gtcgggaagc tcgagcttgg gagcgacctt
     1021 cttgagctcg ctgaccagat ctccagcttc gaagactgcc tgaacgggct ttgagatggc
     1081 cttgtcttcg tccgcctttt tggccttctt gtcatactcg gcgcggtaaa acgcggcgag
     1141 ggcgaccttc gccggctctt ccatcttttt catgacccgc cagccgatat ccagcggcgg
     1201 aatgaacaca ataaccacgc cgacaaacag cccgatcagc ccgagtatca ggcgaacgcg
     1261 cagttcctcg atgtgttcac cgaagctcat ggtgaccatc gattgttctt cggagaaaag
     1321 atcttgatct ttgggcatga cgtgagccac ccgtgtagag gctggatgga aatcgcctgg
     1381 aacggcgaat aggcgaggag acgcgggttg ccacggccag tcccgaggcc ggaacccttc
     1441 taaggcggcg ccacacgtcg gtctctccgg gacgaccaga tgtgcgggag cgttcctact
     1501 agattgaact taccatatat gcgaccgacg tttccagggg atctgtcatt gacgtacgcc
     1561 gaacgaccgg aaaagctctt cgatttgctt tcccgaccag tttgcaaacc cgatcgacca
     1621 ggccaacatg cgtccttcgc gatcgatgac gaaagtctgg ggcatatggt ggatcttgta
     1681 ggccttgggt atcgatccgt cgtgatcgac gtaaacgtgc aggtccggag cgatcgacgc
     1741 ggccacctcg cttgccgtct ttgcgctggt ttcgtcgagg catacaggca gcacaaccaa
     1801 tccggcgttc tcgaatttct tcgccaggag cgctaattcg ggaagttctt ttctgcaggg
     1861 gacgcaggtg gttccccaaa aggcaacgac aacaaatttg cctcgatgtt gctcgagagt
     1921 gcccgtgatc ccatccgcgt cgcgaaactt caccggtgga gcgattgcgc cgccaccggg
     1981 agagagcccg gtcttcttga ccaaggccga gatctccagc gaaagcacgt aatcgacgat
     2041 tgagtccaat tcgccggtcg tcagtacgcc ggccaggcca tgcatcgcct tgtcagcagt
     2101 cccgtcctcg atgacccgcc ggacggctgc gcggtccgtg ccggcgcgcc aagatgctga
     2161 ggcgagatca cgagggcgct gtaggacatc ggtcaaggcc gggccgtcac catggccgtc
     2221 ggcgccgtga caacgctggc agtgcgcctc atacatgggc acgccgcgca cgagcgtgta
     2281 ttcggcgatc tcggccgcca tttcgcgctg gaattgggga ccgttcgccg tcaatgaagc
     2341 ccactcgtcc ctgaaactcc atagcgaatg ataggcgagt acacagccgg tgacggcgag
     2401 accgatcgcc ccaatcagct tggcagctct gcgaacacgc cgacaagcgg gaacgctctg
     2461 gaaaggctcg tcggcaggca ccaggtcact ctgtgtcatc gcgttacctt tcggccgtct
     2521 cgaatgccga caagtcgccc gacgcccacc tcgctcgaag ccgcgtgaac caagtgcgtc
     2581 attatatcac gcgcgggtct gccgtcgatc tcgacggcgc tcgccgccac ctcgacgacc
     2641 taccgtctcc tgaggttcat tgatcagtcg gccaggatcg caaccggcgg ccgtcccgcc
     2701 attcctctga caccgttgca tgaaggactg gtaggagcag accgggctca tccctgggtt
     2761 ctggagcggc tga
//
LOCUS       DATAQL010000424         3281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3095196, whole genome shotgun sequence.
ACCESSION   DATAQL010000424 DATAQL010000000
VERSION     DATAQL010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3095196"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..176
                     /locus_tag="VHS97_07355"
     CDS             45..176
                     /locus_tag="VHS97_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448055.1"
                     /translation="MRNGKRGRIIELKPSVNRTAQELIELLETLEPLWGKVLDAFKR"
     gene            191..613
                     /locus_tag="VHS97_07360"
     CDS             191..613
                     /locus_tag="VHS97_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3448056.1"
                     /translation="MTIRKSIWVARTPEISFKVFCEKITEWWPGGFGGRDSRLFLEGQ
                     VGGRFYEGRADGTEYEIGRITAYEPPSLVAFTWRAPSWEVTTQVEIHFTAEAEGTRVE
                     LEHSGWEQDAKTREARKNYASGWDLILGHYQTAFAATA"
     gene            complement(823..2232)
                     /locus_tag="VHS97_07365"
     CDS             complement(823..2232)
                     /locus_tag="VHS97_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3448057.1"
                     /translation="MPNIEKVENLVIGSGGAGKFVAWTMAGAGRRTIVVERAFIGGAC
                     PNVACLPSKNMIYSAKVISLARRGAEFGLKTESLSVDMERAQRRKRLMVERLRQMHLD
                     RTHASGAELIIGNARFVSPRTVEIELKSGGTRTISGDRVFLDLGSRAAIPEIPGLAEA
                     KPMTHVEALDLDRLPEHLIVLGGGYVGLELAQAFRRFGAQVTVIEEGLQLASREDTDI
                     SAALKDSFVDEGIEVLLGTSVRQVEGRSGQRIKVHATDARGQHSIEGTDLPVATGRMA
                     NTDGLGLEVTGVQLDPHGYVRVNERLETTSEKIWALGDCAGSPQFTHVAVNDFSIVYA
                     NLNGGNRTTKDRLIPFCMFTDPELARVGRNESEARRDGIEYRLAKMPMAEVLRTWTVS
                     EPRGLMKVLIAKDSDEILGFSAFGFEASEQMSAMQTAMLGHLPYTVLRDRILAHPTMS
                     EGLNELLATVPPRNARTSA"
     gene            2836..>3281
                     /locus_tag="VHS97_07370"
     CDS             2836..>3281
                     /locus_tag="VHS97_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3448058.1"
                     /translation="MGVNFSVKDKVAIVTGGSKGIGRAIAITLAEHGADVAVAARGRE
                     ALEKTRSEIEATGRRCIAFTADMADEKEWHRLVDETIAALGGVDILVNNAATASAYGP
                     IAKTDSASWDLVMKVNLKAPFVLSSLCLPSMKARGGGSVIHITSNEA"
BASE COUNT          674 a          967 c          984 g          656 t
ORIGIN      
        1 cgccgggcta caaccgcccg tgactggcca ccgaggccca tttgatgcgc aacgggaaac
       61 gcggacgaat catcgagctg aagccctcgg tgaaccggac ggcgcaggag ctgatcgagc
      121 tgttggagac gctggaaccg ctctggggca aagtgctgga cgcgttcaaa cgataggctg
      181 aggaagagga atgacgattc gcaaatcgat ctgggtggct cgaacacctg aaatatcgtt
      241 caaggtgttc tgcgagaaga taaccgaatg gtggcccggc ggcttcgggg gtcgggacag
      301 ccggttgttc ctggaaggac aggtgggcgg gcggttttac gaagggcgag cagacggcac
      361 cgagtacgag atcgggagga taacggctta cgaaccgccg tcactggttg cgtttacttg
      421 gcgggcgccc agttgggagg ttaccacgca agtcgaaatt cactttaccg cggaagcaga
      481 aggaacccgc gtcgaactgg agcacagcgg atgggaacag gatgccaaga ctcgcgaagc
      541 tcgcaagaac tacgcttccg gatgggacct gatactcggg cactaccaga cggctttcgc
      601 cgccacggca tgaccgaatc gaaacttagc tttttttccg tcgccgcggt ctttcacagt
      661 gggtatcaga ctacacggtt gccacacctt ctatgagcac aacctgttca agcgctgtgc
      721 gcccgtcccc atcctattga aatcgaactc atggcggctg gcttgcgtta ggttcacctc
      781 tcgcgacctt gaagagatcg ccatcggtac aggtgaatcc gttcaagctg aggttcgtgc
      841 gtttctcggc ggaacggtag ccaatagctc gttcagacct tcggacatcg tgggatgggc
      901 caggatccgg tcgcgcagca ccgtgtacgg cagatgacca agcatcgcgg tctgcatggc
      961 gctcatctgt tcgctcgcct cgaaaccgaa cgcggaaaaa cccaaaattt cgtcgctgtc
     1021 ctttgctatc agcaccttca tcaggccgcg cggctcggag accgtccagg tccgtagtac
     1081 ttccgccatt ggcatcttcg ccagccggta ttcgatgccg tcgcgacgcg cctcggactc
     1141 gtttcttccg actcgcgcca gttcggggtc ggtgaacatg cagaacggaa tcagccgatc
     1201 cttggtggtc cgattgccac cgttcaggtt ggcgtacacg atgctgaagt cgttgaccgc
     1261 cacgtgcgtg aactgagggc tgccggcaca gtcgcccagc gcccaaatct tctcgctagt
     1321 ggtctccagc cgttcgttga ccctgacata gccgtgcggg tcgagttgaa cgccggtcac
     1381 ctcaaggccg agtccgtcgg tgttcgccat ccgcccagtc gcaaccggta gatcggtacc
     1441 ttcgatagag tgttgaccgc gggcgtcggt tgcatggact ttaattctct ggccggaccg
     1501 tccctcgacc tgacgaacgc tggtgccgag caggacctcg attccctcgt caacaaacga
     1561 atccttcaac gccgcgctga tatcggtatc ttcgcggctg gcaagttgca gaccctcttc
     1621 gatcacggtg acctgcgcgc cgaatcggcg gaacgcttgc gccagctcca ggcccacgta
     1681 gccgccgccg agcacgatca gatgctcggg taatcggtcg aggtcgagag cctccacgtg
     1741 cgtcatcggt tttgcctctg cgagtccggg gatttctgga atggccgccc gtgaacccag
     1801 gtcaaggaac acgcggtccc cggaaatcgt tctcgtgccg ccgcttttga gctcgatttc
     1861 gacggtgcgt ggggacacga atcgcgcgtt gcctatgatg agttctgcgc cgctcgcgtg
     1921 ggtgcggtcc aggtgcattt gacgtagccg ctcgaccatg agccgtttgc gtcgctgtgc
     1981 gcgctccatg tcaacgctta acgactcggt ttttaggccg aactcggcgc cccgcctggc
     2041 cagtgaaatg accttggctg aatagatcat gttcttgctg gggagacagg caacgttagg
     2101 gcaggcgccg ccaataaatg cccgctcgac tacgatggtg cgacgtcccg cccccgccat
     2161 ggtccaggca acgaacttgc cggctccacc gctgccgata acaagatttt cgaccttttc
     2221 aatgttgggc atccgcgtac ctcctctagt ccaacgatgc aaaaagtttg gatgtcatcg
     2281 aagctcgcgg aaacctcgcg cttcggttgc ttgagcctta ctgaacctag ttggcattta
     2341 gcatatcggg ggtcacctga atctgccgtc aggtctgcgc cggggttggc acgcggtgtg
     2401 gagggtagaa accgtgcgcc gctaaccctt cggccacgga gcccgtctgc tccgttgcgt
     2461 cgccgaaaag caccgagcga cgcgaggtcc gaccgactga tatcacctct atacagcaag
     2521 cggagataat actacgtgag gaaagggctc gacgtcccgc cgcgttattc ggggtaatga
     2581 tatcccgcga aatcgtccat ggtaccgccc cgctatgaga cgtctccacg aaccatgaag
     2641 cggagggaag atccgaaaat cggtccgtct cagtcttcga cacaccagag gagaacgatc
     2701 tagctcgtta tcgcgatctc gggaccgtcg atcgcgcaaa tatctcgatg cctctccgaa
     2761 ggacgcgaca ttgtgctgcg tcggcccgat cggtaagcag aaactgaacg caaactactg
     2821 ccgcggagtc gaatgatggg agtcaatttt tccgtaaaag acaaggtcgc catcgtcact
     2881 ggcggttcaa agggaatcgg ccgcgcgatt gctatcaccc tggccgagca cggagccgac
     2941 gtcgcagtcg cggcgcgtgg gcgcgaagcg ctcgagaaga cgcgttccga aatcgaagcg
     3001 actggccgcc gatgcatcgc gttcacggca gacatggctg atgaaaagga atggcaccgc
     3061 ctggtagacg aaacaatcgc agcgctggga ggcgtggata ttctggtcaa caacgcggcg
     3121 accgcatctg catacgggcc catcgcgaag accgacagtg ccagttggga cctggtgatg
     3181 aaggtgaacc tgaaggcgcc gttcgtcctc tccagcctgt gcctcccttc gatgaaagct
     3241 cgtggaggcg gctcggtcat acacataacc tccaatgaag c
//
LOCUS       DATAQL010000425         3865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1416405, whole genome shotgun sequence.
ACCESSION   DATAQL010000425 DATAQL010000000
VERSION     DATAQL010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416405"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..1008
                     /locus_tag="VHS97_07375"
     CDS             463..1008
                     /locus_tag="VHS97_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3448059.1"
                     /translation="MPTTRITVDEYERMVDSGALTENERVELIRGEIVPKMPIGPKHA
                     ACLKRLIRLLSRRLGDSAIVGSQDPVRLIDSEPEPDISLVRPRSDFYASGHPRPDDIV
                     LIIEVADSSLDDDRNIMRPLYAEAGIAEFWIVNIPEERLEVYRGPRPDGTYSDVQTLG
                     RGQSTGILGLPGIVVGVDEVL"
     gene            1033..3417
                     /locus_tag="VHS97_07380"
     CDS             1033..3417
                     /locus_tag="VHS97_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3448060.1"
                     /translation="MVRSLLAARYAAFTAVVILLLVLAVSGKRVSDEQSINSFFAADD
                     PYMRVYQEAAKTFGDDNFIFLVYDDPELLSPAGLERVTELAAAVAPDRIPAVQRVESL
                     DAMPLLWSIDDALLAIDRLPKMARNLALSAAKRAVKNVDLKSNSMTVAGAVRAAAADQ
                     KALAALKDRVTKHPLFLGTLIDGMGTTTAVVARLKKTHQHNVIETVAEVRRIADEFAA
                     RHQLGPPAIVGPPVLLSDGFAAIEVDGRRLAVVGMILIGVVTLSAVQSLWWAIVPMLA
                     GWVVWLATEAILTTFNIKLSLSGGPLVAQIIVLTMPAASHLAIHFRDERRREADPRQA
                     ARSTLAAVATPIAWTAITGAIGYAALVTSDVVPIQQFGAILGSCTFAAAILVMLISPI
                     AMLPPFRLEIPVRHGSHSPVAGLMNRLILTVYRHPGAIVLAVFAIVLPLAVGMFRLSY
                     ETNYINLFRPETRVVNDYRTVESKLGGIGLVELVVPVGKSLSPANLHDLKVVEDRIQA
                     IRVSDPNAIAQVLSLATVLDPDGRLAALPKERQDRMLADKLELIAASPQNDLLRSFWN
                     TDTGQTRVMIRMKEQMPAPDKSRIFREATAAGAQTFGPTTFLTGLSFLMTRTTEAIIA
                     TQWGTFLWSAIGILLMLTLAFRSLILAVLAIMPTLLSVALVLGLMGWLKIQLDMATAL
                     VASVALGLSVDDTFHCLLQYHRQRKNRFRQRLFESYRVSGPGVILSSLAVAVGFAALR
                     ASEFEPFINFGTMVGIATAGSTLGNLVLLPACLTLGERWRRSKRPRRRPVAARV"
     gene            3653..>3865
                     /locus_tag="VHS97_07385"
     CDS             3653..>3865
                     /locus_tag="VHS97_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448061.1"
                     /translation="MPRVSLYRQGDIPYPQHDGEADSFQDPELSARGHVHARLVQPRR
                     GSQTLACDPTVMVNDCRHETASLSLAI"
BASE COUNT          683 a         1226 c         1178 g          778 t
ORIGIN      
        1 cgatatacct ccgccggcgc gacgccgatc cgcgcgtcgg cccgtccctc ttgagcaatt
       61 tcttcacctg gatcgccttc ttcgcgatta ctggcgtcgc cgtctatagc atgttcgacc
      121 aggtcagggg attaatcacc gggcccggcc agccgtaaga ctctggacga ctccaagacg
      181 cggaaacctt cggttgtgcg acaagcgggg tcgggcgacc cgcgcccaat tccattgaga
      241 cccgcgccga acgagtcgtt gggcgcgagt ttccgacccc acccccaggt gcgaccgaag
      301 atctctcggc gatggcaacc gcccgctgag accgttgtgc ccggaacttg cgattgccgt
      361 ctctgtggtt tgctctcgcc cgtccaccat gcgcaagagt agaattaagc gcgtccgatg
      421 gcctaatgcc aattggatct tcccaggcgt atggaatctc tcatgccgac cacgcgaatc
      481 accgtcgacg aatacgagcg gatggtcgat agcggcgccc tcacggaaaa cgagcgggtc
      541 gagctgatcc gaggggagat tgttcccaag atgccgatcg ggcccaagca cgcagcctgt
      601 ctcaagcgac tcattcggct attgtccagg cggttaggcg acagcgcgat cgttggcagt
      661 caagacccgg tacgcttgat cgacagcgag ccggagccgg acatctccct ggtgcggcct
      721 cgttccgatt tctacgcgtc cgggcatcct cgccctgacg acatcgtcct catcatcgaa
      781 gtcgccgatt cctcgctgga cgacgaccgt aacatcatgc ggcctctgta tgcggaagcc
      841 ggcatcgcgg aattctggat cgtcaacatc ccggaggagc gcctggaagt gtatcgcggc
      901 cctcggccgg acgggacgta ctcggatgtg cagacgctgg ggcggggcca gagtacaggc
      961 atcctgggcc tgccgggcat cgtcgtcggg gttgacgaag tgctctagtg tttcccgagg
     1021 acggagccgt ttgtggttcg ctccttgctc gctgcccgat atgcagcttt taccgcggtc
     1081 gtgatcctgc tcttggtgct tgccgtgtcc ggcaagcgcg tgagcgacga gcaatcgatc
     1141 aattcgttct tcgccgccga cgatccctac atgcgggtgt atcaggaggc agccaagacg
     1201 ttcggcgatg acaacttcat cttcctcgtc tacgacgacc ctgagttgct ttcacccgcg
     1261 gggttggaac gggttaccga gctggcggcc gcggtagcgc cggaccggat ccctgccgtg
     1321 cagcgtgtcg agtcactcga cgccatgccg cttttgtggt cgatcgacga cgctctcttg
     1381 gcgatcgacc gtctgcccaa gatggcgcgt aacctggcct taagtgccgc caaacgggct
     1441 gtcaaaaacg tcgacttgaa atcgaattcg atgaccgttg ccggagccgt gcgggccgcg
     1501 gcggcggacc aaaaggcgtt ggcagccctc aaagaccggg tgacaaagca tccgctcttt
     1561 ttgggcacgc tgatagacgg catggggacg acaaccgcgg tggtcgcccg actgaaaaag
     1621 actcatcagc acaacgtgat cgaaacggtg gccgaagtcc ggcggatcgc cgacgaattc
     1681 gccgcacgcc atcagctggg cccgccggcc atcgtcggcc cgccggttct cttgtccgac
     1741 ggcttcgcgg cgatcgaggt cgacggccgc cgcctggcgg tggtcggcat gatattgatc
     1801 ggcgtggtta ccctctcggc cgttcagagc ctctggtggg cgattgtgcc gatgctcgcc
     1861 ggttgggtgg tgtggctggc taccgaggcg attttgacga ccttcaatat caagctgtca
     1921 ctctcgggcg gccccctggt ggcccaaatc atcgtgctga cgatgcccgc ggcgagccac
     1981 ctggcgatcc actttcgcga cgaacgccgt cgcgaggctg atccgcgcca ggcggcccga
     2041 tcgaccctgg ccgcggttgc gacgccgatt gcctggacgg caatcactgg cgccatcggc
     2101 tatgcggcac tcgtcaccag cgacgttgtc cccattcagc aatttggcgc gatcctcggg
     2161 tcgtgcacat tcgccgcggc gatcctggtc atgctgatct cgccgatcgc catgctgccg
     2221 ccgttccgcc tggaaattcc ggtccgccac ggctcgcact cgccggtggc cggacttatg
     2281 aaccgtctga tcctgacggt ttaccgccat cccggcgcga tcgtcctggc cgtcttcgcc
     2341 attgtgcttc ccctggccgt cggtatgttt cgtctcagct acgaaacaaa ttatatcaat
     2401 ttgtttcgtc cggaaacgcg cgtcgtcaac gattaccgca ccgtagaatc caagctcggc
     2461 ggcatcggct tggttgagct ggtcgtaccc gtcggtaaat cgctctcgcc tgccaacctg
     2521 cacgacctca aagtcgtgga ggatcgtatc caggcgatcc gggtcagcga tccgaatgcg
     2581 atcgcgcagg tgctttcgtt ggcgacggtg ctcgacccgg atggacgctt ggccgcgctt
     2641 cccaaagagc gacaggaccg catgctcgcc gacaagctcg aattgatcgc ggcctcgcct
     2701 caaaatgatt tgctccgcag tttctggaac accgacacgg gccagacgcg ggtcatgatc
     2761 cgcatgaaag agcaaatgcc cgctcccgac aagtcgcgga tctttcgaga agcgaccgcg
     2821 gccggcgccc agactttcgg cccgaccacg ttcttgaccg gtctctcatt cctgatgacc
     2881 cggacgaccg aggcgatcat cgcgacacag tgggggacct tcctgtggtc ggcgatcggc
     2941 atcctgctga tgctaaccct ggcgtttcgc agcttgatcc tcgccgtgct ggccatcatg
     3001 ccgacgctgt tgtccgtcgc cctggtatta ggcttgatgg gctggctcaa gatccagctc
     3061 gacatggcca cggcactggt ggcgagcgtc gccttggggc tttcggtcga cgacacgttt
     3121 cactgcctat tacagtacca ccgccagcgg aagaaccggt ttcggcaacg acttttcgag
     3181 agttaccgag tgagcggacc gggggtgata ctctccagcc tggccgtggc cgtcggcttt
     3241 gccgctttaa gggccagtga gttcgagcca tttatcaatt tcgggaccat ggtcgggatt
     3301 gcgaccgccg gcagcacgct gggcaacctg gttttgctgc ccgcgtgctt gacgctgggc
     3361 gagcgctggc ggcggagcaa aaggccgcgg cgccggccgg tggcggcgcg ggtataaaag
     3421 cgtgcgggtc gcagcgtgag cgcgcctgac tagcccttag gtgggctaac gtgtcaagtt
     3481 caactaccaa aacgggcgaa atacaaagtc gccgatgccg aagagcgtct ggtttgggga
     3541 cttccgggaa tgcgctgcac ggcgatggcc cggcgctttg ggctcagcac gaaaacgtga
     3601 caccattgat caattctcac ttattcgcgg aacatccagc agctattcgg aattgccccg
     3661 cgtttccctc tatcgccagg gcgacatccc ataccctcaa catgatgggg aggccgactc
     3721 ctttcaagac cccgagctct cggcccgagg ccacgtccac gctcgcctcg tgcagccacg
     3781 acggggaagt caaactctgg cgtgcgaccc cacggtgatg gtgaatgact gtcgtcacga
     3841 gacggcgtct ctctcactcg caatt
//
LOCUS       DATAQL010000426         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5386369, whole genome shotgun sequence.
ACCESSION   DATAQL010000426 DATAQL010000000
VERSION     DATAQL010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5386369"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VHS97_07390"
     CDS             <1..797
                     /locus_tag="VHS97_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX3448062.1"
                     /translation="PGRKVVGQVLPRLIPRWGLGLVVCNAENAAGGSGLTVKCYEELL
                     EAGVDVMTMGDHVYRKDEIFQVFDRTGEVLRPANFPPDSPGPELAVVPARDGTMVAVF
                     TVIGRTFMKPVDCPFRASDRILERLGGEARVVIVDIHAEATSEKQLLGRYLDGRVSAT
                     LGTHTHVATADEQILKHGTAYQSDVGMTGPHDGILGRRYDRVLSATLTCVPAHFDVAT
                     GDPRLNGALVTIDPQTGRALTISRVSFDQEDTQRLLTSSSEELREP"
     gene            complement(783..1781)
                     /locus_tag="VHS97_07395"
     CDS             complement(783..1781)
                     /locus_tag="VHS97_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX3448063.1"
                     /translation="MDELLSRYPPWIQPLSTLDSLGGGGGQSGARLWRYRAEKGQLVL
                     RAWPTHGPGRAHLEQVHRWLFATADLGFVPVPFRDRAGRSLQDWHGSLWEVTPWLAGV
                     ADVNCPPALEHLRLALTGLANFHRRLVGEQAQETSPGLRNRRDTLAALVRGGFDVLER
                     AAVRALPHNLEKADVYSWLTLARQLGPVLLVPLEQSADRIIPVQPCLRDARPEHFLFE
                     GDRLSGLVDYGAMGIDSVAGDLARLLGEWLDGDPDARRTAIDSYERLRPLDPAEASLI
                     RLFESSTALLIGERWVRWKYIEGRPFDDPNAFAKGLNRSLNQLRRLDRQVARSGFA"
     gene            complement(1834..>2485)
                     /locus_tag="VHS97_07400"
     CDS             complement(1834..>2485)
                     /locus_tag="VHS97_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3448064.1"
                     /translation="NPEVRGFLRVLAALNTLYCSVWHQLAIGRYAPLPECGPAILISN
                     HTCGIDHMLLQSRSRRLLGFMVAREYYEWKWIHWICKYIGCIPVNRDGRDFAATRAAL
                     RALEEGRVLPIFPEGHIVPASGTKLDDMKPGTAYLAIRAQVPVIPAYIHGTPWTNEII
                     ESLVTPSRAHVIFGEPIDLSDVHRDRAGDKKAQAEVSERFKRAFLALQARAFSCEE"
BASE COUNT          492 a          790 c          765 g          438 t
ORIGIN      
        1 cgccaggccg caaggtcgta ggccaggttt tgccgcgtct catccccaga tgggggctgg
       61 gactggtcgt ttgcaatgcc gagaatgccg cgggcggctc cggcctgacg gtcaagtgct
      121 atgaagaatt gctcgaggcc ggcgtcgacg tgatgacgat gggggatcac gtttatcgca
      181 aggacgagat cttccaggtc ttcgatcgta caggcgaggt gctgcgtccc gccaatttcc
      241 cgccggattc gccgggcccg gaactagcgg tcgtcccggc gcgcgatggc accatggtcg
      301 ccgtcttcac cgtgatcgga cgtactttca tgaagccggt cgattgcccg ttccgggcca
      361 gcgaccggat cctcgagcgg ctgggcggag aggcgcgcgt cgtcattgtc gacatccacg
      421 cagaagcgac cagcgagaag caacttctgg ggcgctatct tgacggacgg gtatcggcca
      481 cccttggcac gcacactcac gtcgccacgg ccgacgaaca gatcttgaaa cacgggacgg
      541 cctaccagtc cgacgtcggc atgaccggac cgcacgacgg gattctgggc cggcgctacg
      601 atcgcgtctt gtcggcaaca ttgacctgcg tcccggccca tttcgacgtc gccaccggcg
      661 acccacgact aaacggcgct ctcgttacca tcgatcccca aaccggtcgc gcactcacga
      721 tcagccgggt cagcttcgat caggaagaca cgcagcgact acttaccagc tcgtctgaag
      781 aactacgcga accctgatcg cgccacttgt cgatccagcc ggcgcagttg gttcaggctt
      841 cgatttagcc ccttggcgaa cgcattcggg tcgtcgaaag ggcggccttc gatgtacttc
      901 caccgtaccc atcgctcgcc gatcagtagg gctgtcgacg actcgaaaag acgaatcaag
      961 ctggcctccg ccggatcgag cggccgaagc cgttcgtagg aatcgatcgc tgtgcggcgg
     1021 gcgtctggat cgccgtccaa ccactcgccc agcagccgcg ccaggtcgcc cgccacgctg
     1081 tcaattccca tggcgccata atcgacgagg ccgctcaggc gatccccttc gaagaggaaa
     1141 tgctcgggac gcgcgtcacg taaacacggc tgcactggaa tgatccggtc cgccgattgt
     1201 tcgagcggca caagcaagac cgggccgagc tggcgcgcca acgtgagcca ggaatacacg
     1261 tcggcttttt ccagattgtg cggaagggcg cgtaccgcgg ccctttcgag cacgtcgaac
     1321 cctcctcgga ccaaggccgc gagcgtgtcg cgccggtttc gtagccccgg tgacgtttcc
     1381 tgagcctgct cgccgaccag tcgccggtga aagttcgcca gtccggtcag ggcaagccgc
     1441 agatgctcga gagcaggggg gcaatttaca tcggcaaccc cagccagcca gggtgtgact
     1501 tcccagagcg aaccgtgcca atcctgcaga gaccgacccg cgcggtcgcg gaaggggacc
     1561 gggacgaatc caaggtcggc agtcgcgaac aaccagcgat ggacctgttc cagatgggcg
     1621 cgtcccggac catgcgtggg ccaggcgcgc aaaacgagct gacctttctc ggctcgatac
     1681 cgccagagac gggcgccgct ctgaccgccg ccgccgccca gcgaatcgag cgtcgacagc
     1741 ggttgtatcc agggtggata gcggctgagg agctcgtcca tggatctctt gctcagatca
     1801 atgaaggcaa tctgggagtt tcacgccatt cattcactct tcacacgaga acgcacgcgc
     1861 ctgcaaggcg agaaacgctc gcttgaaccg ctcggacacc tccgcctgcg ctttcttgtc
     1921 gccggctcgg tcacgatgga cgtcggaaag atcgatgggc tcgccgaaga tcacatgagc
     1981 ccgcgaagga gtcacgagcg actcgatgat ctcgttagtc cacggtgtcc cgtggatata
     2041 ggctggtatt acgggcacct gtgcgcggat cgccagatac gcggttccgg gcttcatgtc
     2101 atcaagtttc gtgcccgacg ccggaacgat gtggccctcg ggaaagatcg gcagtacccg
     2161 gccctcctcg agcgcccgca gcgccgcccg agtcgcggcg aagtcgcggc cgtcgcgatt
     2221 gacggggata catccaatat acttacaaat ccagtggatc catttccatt catagtactc
     2281 tcgagcgacc atgaaaccta gcagccgtcg gctccgtgat tggagcagca tgtggtcgat
     2341 accgcaggtg tgattcgaga tcaggatcgc cggaccacat tcgggcaggg gggcgtatct
     2401 gccaatagcc aattgatgcc aaacggaaca gtagagtgta ttaagcgctg ccagcacgcg
     2461 caaaaagccg cggacctccg gattg
//
LOCUS       DATAQL010000427         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3555210, whole genome shotgun sequence.
ACCESSION   DATAQL010000427 DATAQL010000000
VERSION     DATAQL010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555210"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VHS97_07405"
     CDS             <1..732
                     /locus_tag="VHS97_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX3448065.1"
                     /translation="GNFLVKVSVEVLWLAILVAFFRVVFARTSMIAGWSEPQYFFFVG
                     CFFAMNGLIEMLFLDNCNEFAELVRTGDLDGLLLKPIDEQFLITCRRIDWGTAPNILM
                     GAIVMVISLFLMHWHFDLARVLTFAITFVCGTAIAYSFMLLLTSISVWMVRNQSLMEM
                     WWLFSSVARYPKEIFAGRWAEPIGAFFTYMMPILIVVNVPANAMVRALDPAMVGLTLA
                     ASAILLWLSRRFFQHALRSYRSASS"
     gene            738..2192
                     /gene="rsmG"
                     /locus_tag="VHS97_07410"
     CDS             738..2192
                     /gene="rsmG"
                     /locus_tag="VHS97_07410"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX3448066.1"
                     /translation="MRPAAQSLEAILTRCGIALDGEQIQLLWRYHQMLRKANAALNLT
                     RIHNFENMVLKHYVDSLLVLKFVDLPAPLIDMGSGPGLPGIPLKLARPQVPMILAEPR
                     GARAMFLREVCEQLELTGVEVYDHKIGADYPGRVGGVITRAVGSIAETLDRVSSCLGP
                     GGRMIFMKGPDCDQEITEAAESHAGLFRILSDHAYTIPGTTHDRRCVVYERLEGEADR
                     GRSRKSLREAAVSAEFAGTVREITSASNPTFQRCRVLFSGPGIRKHGEAILSGTRIYS
                     EVLTRFPDQVAAWMTDTRGQPPPTSSIEWLRFSDPLFKELDVVGTHAPLLLVRVPAMA
                     AWNDGNPWPDGCTLFVPFQDPENIGAVIRSAAAFGVARVVLLREAAHPFHPRSARAAG
                     PALFQIPLEQGPALEELRVASVPLIALDTTGPELSDAPFPDTFGLVVGVEGPGLPARL
                     RQCQRRRIDMQPGVESLNAATAAAIALYVWSRHR"
     gene            2364..3293
                     /locus_tag="VHS97_07415"
     CDS             2364..3293
                     /locus_tag="VHS97_07415"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX3448067.1"
                     /translation="MAEELKLKNPWMVTVWPGIGNVAISAGYYLMAKLGMHLLAEFAA
                     QELFDVEQVEVKDGLIQSARLPRSRFFVWNDPREQHDLIVFIGEAQPPMGKYTFCQRL
                     IEFAKTLGVERVYTFAALVTQMNPARPSRVFVAATDVECLKELKRLGLETIENGQIGG
                     LEGVLLGVAVENGMRGACLMGEMPHVLAQLPSPKASLAILEAFGSMADLHVDYAELAE
                     QVRNMEQKLGEFLEQMQKSIESDTPAEESSIADESVEDEGPGPDGRRRIEQLFAQAKE
                     DRATAYELKRELDKLGVFREYENRFLDLFKKRE"
     gene            3422..3721
                     /locus_tag="VHS97_07420"
     CDS             3422..3721
                     /locus_tag="VHS97_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448068.1"
                     /translation="MHVAHAGTSEVAIFGRVLESDQATLDVAAARAILNLDFKQSDKD
                     RTRELLAKAKKGTLTADEQVAIDNYERVGHMLSLMKLKARRSLRGKRANGAAKAR"
     gene            3728..>4008
                     /locus_tag="VHS97_07425"
     CDS             3728..>4008
                     /locus_tag="VHS97_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX3448069.1"
                     /translation="MERALEHLVWQRAGGRFEYCQICSDNLDLPFEVDHIIAEHHLGL
                     TDAKNCCLACYACNRHKGPNVAGVDPKTKSIVPLFHPRRQKWSRHFRWDG"
BASE COUNT          784 a         1162 c         1208 g          854 t
ORIGIN      
        1 ggcaatttcc tggtcaaggt ctccgtcgag gttctctggc tggcgatcct ggtcgcgttt
       61 tttcgcgtgg ttttcgcgcg cacaagcatg atcgccggct ggtcggagcc ccagtatttc
      121 ttctttgtcg gctgcttctt cgccatgaac gggctgatcg agatgctctt cctggacaat
      181 tgcaatgagt tcgccgaatt ggtgcgcacc ggcgatctcg atggtctatt gctcaaaccg
      241 atcgacgaac agtttctgat cacctgcagg cgcatcgatt ggggcactgc ccccaatatt
      301 ctgatgggcg cgatcgtaat ggtcatctcg ctcttcttga tgcactggca ttttgacttg
      361 gcgcgggtgc tgacctttgc catcacattc gtctgtggca ctgcaattgc ctacagtttc
      421 atgctgcttt tgacctcgat ctcggtgtgg atggtacgta accaaagctt gatggaaatg
      481 tggtggctgt tttcgagtgt ggcgcgctat cccaaggaaa tcttcgcagg tcgctgggcc
      541 gagcctattg gcgccttttt cacgtatatg atgccgatcc tgatcgtcgt caacgtgcct
      601 gccaatgcga tggtccgcgc tctcgacccg gccatggtcg gcctgacttt ggccgcgtcc
      661 gcgatcttgc tctggctcag ccgtcgcttt tttcagcacg ccctgcggtc gtaccgcagc
      721 gcgagtagtt gacacagatg agacccgcag cccagtcgct cgaagccatc ctgacgcgtt
      781 gcggcatcgc gctcgatggg gagcaaatcc aattgctctg gcgctaccat cagatgttgc
      841 gtaaggccaa tgccgctctc aatctgacgc gaattcacaa tttcgagaac atggtgctca
      901 agcactatgt cgacagcttg ctcgtgctca aattcgtgga tctgccggcg ccgttgattg
      961 acatgggctc gggaccgggt ctgccgggaa ttcccctcaa gctcgctcgg ccccaggtgc
     1021 ccatgatcct ggccgagccc cgaggtgcgc gcgcgatgtt tctccgcgag gtttgcgaac
     1081 aactggaact aacaggcgtg gaagtctacg accacaagat cggcgccgat tatcccggcc
     1141 gagttggtgg tgtcatcacc cgcgcggtcg gatcgatcgc ggagactctc gaccgcgtat
     1201 cgtcgtgttt ggggcctggc ggccggatga tctttatgaa gggacccgat tgcgatcaag
     1261 agattacgga agcggcggaa tcgcacgcag gtctcttccg cattctgtcc gatcacgcgt
     1321 acacgattcc cggcacgacg cacgaccggc ggtgcgtcgt ttatgagcga ttggaaggcg
     1381 aggcggaccg aggccggtca agaaagtcgc tgcgggaagc cgctgtttcg gccgagttcg
     1441 ccggcacggt tcgagaaatc accagtgcct ccaacccgac ctttcagcgc tgtcgcgttc
     1501 tcttctccgg gcccggcatc cgtaaacacg gcgaagcgat tctgtccggc acgcgcatat
     1561 attccgaggt gctgacgcgg ttccccgacc aagtcgccgc ttggatgacc gacacgcgcg
     1621 gccaacctcc gccgacgagc tcaatcgaat ggttacggtt cagcgacccc ctgttcaaag
     1681 agctcgacgt cgtggggacg cacgcaccgc tgctgctggt gcgtgtgccc gcgatggctg
     1741 cttggaatga tggcaaccct tggcccgatg gctgcacgct tttcgtcccg tttcaagatc
     1801 cagaaaacat cggcgcggtg atccgctctg ctgccgcttt tggcgtagcg cgcgtggttt
     1861 tgttgcgcga ggcggcccac ccgtttcatc cccgaagcgc acgggcggcc ggtccggcgc
     1921 tctttcagat tccgctcgag caagggccgg cgctcgagga gctgcgagtc gccagcgtcc
     1981 ccttgattgc gctcgatacg accggacctg agttaagtga cgccccgttt cccgacacgt
     2041 ttgggctggt tgtgggtgtc gaaggacccg ggttgcccgc acgtctgcgc cagtgccagc
     2101 gccggcggat cgatatgcag cctggcgtcg agtcgctcaa cgcggccaca gccgccgcga
     2161 ttgcccttta cgtctggtcc cgccaccgct gaccgcctga gggcggtcaa agggtgcggt
     2221 tgagcgacaa cccgagtgaa taataccgga tatgcctact ggttttggaa ggccatgggg
     2281 agagagatgg gctcttgtcc ggaatccgcg gcgatgacat gatcgctcta aggccagcac
     2341 gaccgaattg ggggaacacg atcatggctg aagaactaaa actcaagaat ccttggatgg
     2401 tcaccgtctg gcctggaatc ggcaatgtcg ccatcagcgc cggctattac ttgatggcga
     2461 aactcggcat gcacctactg gccgagttcg cggcgcaaga gctctttgac gtcgaacaag
     2521 tcgaagtgaa agacggcctg attcaatcgg cgcgcttacc gcgcagccgg tttttcgtgt
     2581 ggaatgaccc tcgcgagcaa catgatctca tcgtgttcat cggcgaagca cagcctccca
     2641 tgggaaagta tacgttctgc cagaggttga tcgagtttgc caagacgttg ggcgtcgagc
     2701 gggtttatac tttcgcggcc ttggtgaccc agatgaatcc cgcccgtccc tcgcgtgtct
     2761 tcgtcgccgc caccgacgtc gaatgcttga aggagctgaa gcgtctgggt ttggagacga
     2821 ttgaaaacgg ccagatcggc ggcctcgagg gcgtgttgct cggtgtggcg gtcgagaatg
     2881 gaatgcgtgg cgcctgtctg atgggggaaa tgccccacgt cctggctcag ctcccatccc
     2941 ccaaggcttc attggcaatt ctcgaagcct ttggctcgat ggccgatctc catgtggatt
     3001 acgccgagct ggccgagcaa gtcaggaaca tggaacagaa gctcggtgag ttcctggagc
     3061 aaatgcaaaa atcgattgaa tcagacacgc cggccgagga atcatcgatc gccgacgaat
     3121 cggtcgagga cgaaggaccg ggccccgacg gccgacggcg gatcgagcag ctctttgcgc
     3181 aagccaagga agaccgcgcc accgcctacg agctcaagcg cgagctcgac aagctcggtg
     3241 tctttcgcga gtacgagaat cggttcctgg acctttttaa gaaacgcgag tgaagcgcaa
     3301 gttgcattca cgtccgtagc ccaaacgaag ccaacgtcgc ggcaagcgtt agttattgcg
     3361 gaaaaaccag ctatattgct acactgaagc ctgctaagtc aacgtacgaa atggagcggt
     3421 catgcacgtc gcccatgcag gcaccagcga agtagcaatt ttcggtcggg tacttgaatc
     3481 cgatcaggca accctcgatg tcgcagcggc gcgggcaatc ctcaacctcg acttcaagca
     3541 gagcgacaaa gaccggacgc gtgagttgtt ggccaaggcc aagaaaggga cgctcacagc
     3601 ggacgagcag gttgcgatcg ataactatga gcgggtcgga cacatgctgt cgctgatgaa
     3661 gttgaaggcc cgacgatcgc tcaggggcaa gcgggccaac ggtgcagcca aggcgcggtg
     3721 agttcgcatg gaacgcgcgc tggagcacct cgtgtggcag cgagccggcg gtcgattcga
     3781 atattgccaa atctgttcgg ataatctcga tcttcccttc gaagtcgacc acatcattgc
     3841 cgaacatcac ctgggcctga ccgatgcaaa gaactgttgc ctggcctgtt atgcgtgcaa
     3901 ccgccacaaa gggccaaatg ttgccggggt tgatcccaag acgaagagca tcgttccgct
     3961 gttccatccc cgtcgtcaaa agtggtcacg gcattttcgc tgggatgg
//
LOCUS       DATAQL010000428         5141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4316759, whole genome shotgun sequence.
ACCESSION   DATAQL010000428 DATAQL010000000
VERSION     DATAQL010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4316759"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VHS97_07430"
     CDS             <1..1532
                     /locus_tag="VHS97_07430"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448070.1"
                     /translation="VIHQETRRDALGRVIAQTEGEVRLVVGSGRLGIAYLIERDGFLF
                     HSPIAWYRREGRWDLPPGYEKSNPHFDRPIQSVCLFCHANRSEPVEGTVNRYQPPFFA
                     GHAVGCERCHGPGELHAARPNLIDGRDLTIVNPAALEPALRDAVCQQCHLNGQKRVLR
                     AGRRDEDYRPGLPFQHFWTVLERPGSLEENRLVGQFEQMHQSRCFQASQGRLGCITCH
                     DPHQLPEPDENLAYFRDRCLECHSDRGCSLPATVRTEQGRADDCVRCHMPRMNNSDII
                     HVAETNHRIPRRADETKRTGTKAGNSNGNLRPWVNFHRDLMTERERDEAERDVGVALC
                     LDGPIGAATALPALEDALAAWPDDVPGWEAKGYALGLLHRDQEGLSAFRKALALEPNR
                     ESALTGAAFSAAHAGRRDDSIAYWQRAIGLSPWRAEYRAELASLYFQSREWHAATSAC
                     REALRLDPANLDVRKLLVRSLLRLGDNSAAQGEFETLIAFDPPDRDRLLGWFTTLTKS
                     R"
     gene            1605..2396
                     /locus_tag="VHS97_07435"
     CDS             1605..2396
                     /locus_tag="VHS97_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX3448071.1"
                     /translation="MNPVRIALANIRFPAAPEESITLAEAAIEEASKQGARVICFPEC
                     FVPGYRGIGKPVPPADPEFLDRAWSAIARAAAKANITVVLGTERVVDGALVATALVID
                     QDGAIAGFQDKVQIDPSEEGTYTPGSGRRVFQAGAMTFGIAICHEGWRYPETVRWAAR
                     RGAQVVFHPHFHEAEPDSYRPATFADPANTFHEKAALCRAAENTCYFATVNYAGVGSP
                     TTSAIVRPDGTLLCYQPYGEEGLLIADIDITTATGLLAARCRTVF"
     gene            3389..>5141
                     /locus_tag="VHS97_07440"
     CDS             3389..>5141
                     /locus_tag="VHS97_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3448072.1"
                     /translation="MKFVYLATFALLFFPTVVFGQAKEDDADKVSNDKPDRPLQVPPA
                     SSEVKEAFDDYERFRRRGAWERALKSLYAIPEEQTGRFVDGQNGYIIPVARKRRAVLS
                     ELPPDGLATYRLFYDDPAKKLLEQAEGPTELKTLERIFSAYFLTSVGDNAADRLGDLY
                     FEMGRFDRAADCWLAVLREHPDSDLSPALISVKSALALARAGRRAEIAAIRHDLQERY
                     ADEKISIGGRVAPAAEHLARCLSDERGSKDPATNDSESAGSVEASGIEPDLSRPVPAL
                     WQMRFGESVVAGMTPPERTQWESNPLSAAVPAVAVHGNVLYANFLGYIFALNLETGKL
                     IWRSASFHNLEVPASQGQARMLDTKRFAILASKTHVWTLGRDLKDPNQFGSFVLTCRR
                     ADGGDVTWQSSNLPDYAQVDLVSTPILAGETLYVVGKTSTNQQQGQPHQYVLAIRALD
                     GKLLWKVEVGTFRQMQNQMFMFYGMPDDSPQPKLFKHAGSIYVDTHVGILARLDAESG
                     ELDWGYGYQTAPVEGSRFFYRRMQQKEMSASSVPLKAGEALILKGAKSGRIHALDSDR
                     MKMLWDRPIAESARLLGA"
BASE COUNT         1033 a         1595 c         1509 g         1004 t
ORIGIN      
        1 atgtcattca ccaagagacg cgacgggatg ctctcggccg cgtcatcgcc cagaccgaag
       61 gtgaggtccg gttagtggtc ggctcaggca gactgggaat tgcctacctg atcgaacgcg
      121 acggattcct gttccactct ccgatcgcct ggtatcggcg ggaagggcgc tgggacctgc
      181 cacccggcta cgagaagtca aacccgcatt tcgaccggcc gatccagtcg gtatgcctgt
      241 tctgccatgc caaccgttcc gagccggtgg agggaacggt taaccgttac cagccgccct
      301 tctttgcggg ccatgccgta ggttgcgagc gctgccacgg tccgggcgaa ctgcacgctg
      361 cgcgtccaaa tctgattgat ggccgcgatt tgacgatcgt gaatcccgcg gcactggaac
      421 ccgcacttcg ggatgccgtc tgccagcaat gccacctgaa cgggcagaaa cgcgtcttgc
      481 gtgcaggccg gcgcgatgaa gactatcggc cgggcttacc ctttcaacac ttctggacgg
      541 tgctcgagcg tcccggcagc ctcgaggaaa accgtttggt tggccagttc gagcagatgc
      601 accagagccg gtgcttccag gcaagccagg gccgactcgg gtgcatcaca tgccacgatc
      661 cacatcagct tccggagcct gacgagaatc ttgcttactt ccgagatcgc tgcctggagt
      721 gccactccga tcggggttgc agcctgccgg cgaccgtccg gacggagcag ggacgggccg
      781 acgactgcgt tcgttgccac atgccgcgca tgaacaactc agacatcatc catgtcgccg
      841 agacgaacca ccggatcccc cggcgcgcgg acgagaccaa acgaaccggg accaaggccg
      901 ggaactcgaa cggcaacctt cggccgtggg tgaatttcca ccgcgacctg atgactgagc
      961 gggaacgtga cgaagccgag cgcgatgtcg gcgtggccct ttgcctcgat ggccccatcg
     1021 gcgcggcgac ggcccttccc gcgctcgaag atgccctggc cgcatggccg gacgacgtac
     1081 cgggctggga ggccaagggg tacgcgcttg gcctgctcca ccgggaccag gaggggctct
     1141 ctgcgttccg taaagcgctc gcgctggagc cgaaccggga atctgcgctc accggcgcgg
     1201 ccttttccgc ggcccatgcg ggccgtcgcg acgactcgat cgcgtactgg cagcgcgcaa
     1261 ttgggctcag cccctggcgg gcggaatatc gcgcggaact ggcatctctt tacttccaaa
     1321 gtcgcgaatg gcacgccgcg acgtcggcat gccgagaagc cctacgcctc gaccccgcca
     1381 acctcgatgt cagaaaactg ctcgtccggt ctcttcttcg tctcggagac aactcggcag
     1441 cacaaggcga attcgagact ttgatcgcgt tcgacccgcc agaccgcgat cgacttctgg
     1501 gatggttcac cactttgacg aagtctcggt aacaagtgtc atcccgaatc gcttcgatcc
     1561 gcaatcggtg cgagggaggg ggactctttt ccagagggga actcgtgaat cctgttcgaa
     1621 tcgcactcgc caatatcagg ttcccggctg ctccggagga gtcgatcacg ctcgccgagg
     1681 cagccattga agaagcctcc aagcagggag cacgggtcat ttgctttccg gaatgtttcg
     1741 tcccgggcta tcgaggaatc ggcaagcccg tgccgcctgc cgatcctgag ttcctcgacc
     1801 gcgcctggtc agcaatcgcc cgagcagccg ccaaggcaaa catcacggtc gtgctcggaa
     1861 ccgaacgcgt tgttgacggc gccttggtag cgaccgccct ggttatcgat caggacggag
     1921 cgatcgcggg ctttcaggac aaagtccaga tcgacccctc ggaagagggc acctacacgc
     1981 caggctctgg ccgccgcgtc tttcaagccg gcgcgatgac tttcggtatt gcaatctgcc
     2041 acgaaggctg gcgatatcca gagacggtcc gctgggccgc acggcgtgga gcacaggttg
     2101 tgtttcaccc gcacttccac gaagcggagc ccgattctta ccggccagcg accttcgccg
     2161 atcccgcgaa tacctttcac gaaaaggcgg ccctgtgccg cgccgccgag aacacctgct
     2221 atttcgcaac ggtcaattac gcaggtgtcg gctctccgac cacgtcggcg atcgtccgtc
     2281 cggatgggac attactctgt taccagccct atggtgagga aggattgctg atcgccgaca
     2341 tcgacatcac cacggcgact ggcctccttg cggcacgttg cagaactgtc ttttgatggc
     2401 gcgatccccg ggcttctgat cggttcggtt ctgctgttga attctgatgc ctgactcagg
     2461 cgagccctac cacccaggcg atcttcggga ccaatcgtat gctacccaat cacgtatgct
     2521 aaaagggttt ccggagtcgt taacaaaaat gccataacca taaattgaag ttgagtcggc
     2581 ctggaaagcc catagccgct ggccattcgc ttgcctgaag atccaataaa agacaccggg
     2641 tcgagtcggg ctcagcatcg tctgcttgaa ctctaggtct acattcgtcg tgactgtgcc
     2701 gctcacgaaa atcgcctgtc cccgctgccc cggagtaagc tcaaagaact cccgatcata
     2761 gttgaccggt tggtgaatga cgtatccctt tgactcgtac aaatactcac cgcggaagac
     2821 ccgttgagct gttccggctg cgtcgttcac ggtgacgact ctaccgagcg gagccttttc
     2881 cacggccggc tccggctggt tctgagcgat cgatactgta cttgtcgccc cgagcgccaa
     2941 tcccgccaca cagaaacgaa ccactcgagc gacagctgag ctcacgatgt gcctccggaa
     3001 gatcgtcgaa agggggagtc gttgcggaaa aaaagaaccg tcaacgcctg cctgttcggt
     3061 attacccatt ttatccatta taccataccg taagaatcgt caaattcttt tgggaaatac
     3121 ttcggaatct ctgcagtttc gtcgatggtg tcagaccgag ctgcaactgc cacggatatc
     3181 ggcgatcaac tggcattgac cagcgaattt taagaaattc acttgcatcg actcttacct
     3241 tcgacgaaag taagacgctg gtcccgtaat cgatcgaaag tcctcgatct gacggggcaa
     3301 cttacgtttg tcggcctttg catcggaatt agccgacagg cgcgatttga agccgcaagg
     3361 gaaacgatct caagcgcaac cgggtgacat gaaattcgta tatctcgcaa cctttgcact
     3421 cctattcttt ccgacggttg tcttcgggca ggccaaggaa gacgatgccg ataaggtttc
     3481 caacgacaaa ccggatcgcc ccttgcaagt gccgccggcg tccagcgaag tgaaggaagc
     3541 cttcgacgac tacgaacgat tccgcagacg cggggcttgg gagcgggctc ttaagtcgct
     3601 ctatgcgatt cccgaagagc agaccgggcg gttcgtcgac ggacagaacg gctacatcat
     3661 cccggtcgcc agaaagcggc gagcggtgct cagcgaactc ccccccgacg gattggccac
     3721 ttaccggctc ttttacgacg accccgcgaa aaagcttctt gagcaggccg aaggaccgac
     3781 tgagttgaag acgcttgaac ggatcttctc ggcctatttc ctcacgagcg tcggcgacaa
     3841 cgccgccgac cgtctgggtg acctctactt cgagatgggg cggttcgacc gtgcggccga
     3901 ctgctggctg gcggtcctgc gagaacaccc cgactccgac ctctcgcccg ccttgatctc
     3961 cgtgaagtcg gccctcgcgc tcgcccgcgc ggggcgtcgc gccgaaatcg ccgcgatccg
     4021 tcacgacctg caggagcgct acgccgatga aaaaatatcg atcggcggtc gtgtggcgcc
     4081 ggccgccgaa catctggccc gttgcttgag cgatgaacgc ggctcgaaag acccggccac
     4141 caatgactcc gaatctgcgg gctcggtcga agcgtccggg atcgagcccg acctttcgcg
     4201 gccggttccc gcattatggc agatgcggtt tggcgagtcg gtggtcgccg gcatgacgcc
     4261 cccggaacgg acccagtggg aatcaaaccc gttgagcgct gccgttcccg ccgtggctgt
     4321 ccatggaaac gttctctacg ccaactttct gggttatatt ttcgccctga atctggaaac
     4381 cggtaagctg atctggcggt ccgcctcgtt ccacaacctg gaggtccccg cgtcccaggg
     4441 ccaggctcgg atgctcgaca caaaacggtt cgcgatcctg gcatcaaaga ctcatgtctg
     4501 gacgctgggc cgcgatctca aagacccgaa ccagttcggg tcgttcgtgc tcacatgccg
     4561 aagggccgat gggggcgacg ttacctggca gtcatccaac ctgcccgatt acgcgcaggt
     4621 cgatctcgtc agcaccccca tcctggccgg cgagacgctt tatgtcgtcg gcaagacatc
     4681 aacgaaccaa caacagggtc agccgcatca gtatgttctg gcgatccgcg ccttggatgg
     4741 gaagctgctc tggaaggtcg aggtcgggac attccggcaa atgcaaaatc agatgtttat
     4801 gttttatggc atgcccgacg actcgcctca gcccaagctg ttcaagcatg ccggttcgat
     4861 ttacgtcgac acacatgtcg gcattctcgc gcgactcgac gccgagtcgg gcgaattgga
     4921 ctggggctac ggttatcaaa cggcgcccgt cgaggggagc cgcttctttt accgaaggat
     4981 gcagcagaaa gagatgtctg ccagcagtgt gcccttgaag gcaggcgagg ccctgatctt
     5041 gaagggggca aaatcggggc ggatccacgc tctggattcg gaccggatga aaatgttgtg
     5101 ggatcggccg attgccgagt cggcgcgcct tctcggggcc c
//
LOCUS       DATAQL010000429         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2484929, whole genome shotgun sequence.
ACCESSION   DATAQL010000429 DATAQL010000000
VERSION     DATAQL010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484929"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /gene="atpA"
                     /locus_tag="VHS97_07445"
     CDS             complement(<1..1619)
                     /gene="atpA"
                     /locus_tag="VHS97_07445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX3448073.1"
                     /translation="MKFKADEISSVIQREIEQFSPEVSTSEVGQVLEVGDGIARVYGL
                     SGLMSGEMVSFENGVKGIALNLEESSVGVMVLGDYTGIEEGDTVTATGALLRVPVGEA
                     IIGRVVNALGEPIDEQGPILTDKTWALESVAPGIAARQPVDEPMQTGIKSIDSMTPIG
                     RGQRELLIGDRKTGKTAIAIDTILNQKGTGVICVYVAIAQKESTTAALVDILRRHGAL
                     DYTIVVAAGASDPAPMQYISPYAGCAMAEYFMYEQGKPTLVIYDDLSKQAVAYREVSL
                     LLRRPPGREAFPGDIFYAHSRLLERSAKLANHYVILPETAATDNVTDDMGVDKKFYVG
                     IPGLEEANHALKAHFPTGHKVVKTTRSGGSLTALPIIETLEGEVSAYIPTNVISITDG
                     QIYLQPDLFFAGVRPAIDVGISVSRVGGKAQIPAMKKVAGGLRLDLAAFRELEAFAQL
                     GTELDKATQKQLDRGYRMVELLKQPQYKPMNAIDQVMSIFAGTQGHLDDVPRVDVPRW
                     ETSFLNFIKDRKPEVRAALEREQKMTPKIEA"
BASE COUNT          291 a          507 c          535 g          348 t
ORIGIN      
        1 tcggcctcga tcttgggagt catcttctgt tcgcgttcca atgcggcgcg cacctcgggc
       61 ttgcggtcct tgataaagtt caagaatgag gtttcccagc gcgggacgtc gacgcgggga
      121 acgtcgtcca agtgtccctg agttccggcg aagatactca tgacctggtc gatggcattc
      181 atgggcttgt actgcggctg cttgagcaac tcgaccatgc gatagccgcg gtcgagctgt
      241 ttctgagtgg ccttgtccag ctcggtgcca agctgggcga aggcttcgag ctctcggaag
      301 gccgccaggt cgaggcgtag accgccggcg acctttttca tggccgggat ctgggccttg
      361 ccgccgacgc gcgagacact gatgccgacg tcgatcgcgg gccgcacgcc ggcgaagaag
      421 aggtcgggct gaaggtagat ctggccgtcg gtgatcgaaa tcacgttggt ggggatgtag
      481 gccgaaacct ctccctccag ggtctcgatg atcggcaatg cggtgagcga accgcccgaa
      541 cgcgtggtct tgacgacctt gtggcccgta ggaaagtgcg ctttgagggc gtgattggcc
      601 tcttcgagac cgggaatgcc gacgtaaaac ttcttgtcga cgcccatgtc gtcggtgacg
      661 ttatcggtcg ccgccgtttc gggcaggatc acataatggt tggccagctt ggcggagcgc
      721 tcgagcaacc gcgagtgcgc gtagaagata tcgcccggga acgcctcgcg gccgggaggc
      781 ctgcggagta acagcgaaac ctcgcgatat gccacggcct gcttggagag gtcgtcgtag
      841 atcaccaatg tcggcttgcc ttgctcgtac atgaagtact ccgccatggc acagccggcg
      901 tagggggaga tgtactgcat gggtgccggg tcgctggcac cggctgcgac cacgatcgtg
      961 tagtcgaggg caccatggcg gcgcaagata tcgacgagcg ccgcggtggt cgattccttc
     1021 tgggcaatcg ccacgtagac gcagataacg cccgtcccct tctggttcaa gatggtgtcg
     1081 atcgcgatgg cggtcttgcc cgtcttgcgg tcgccgatca agagctcgcg ctggccccgg
     1141 ccgatgggcg tcatcgagtc gatcgacttg atgcccgtct gcatcggctc gtcgaccggt
     1201 tggcgggcgg cgatacccgg tgcgaccgat tcgagggccc aggtcttgtc ggtcaagatc
     1261 ggtccctgct cgtcgatcgg ctcgcccagg gcgttgacca cgcggccgat aatcgcctct
     1321 cccaccggca cgcgcaaaag cgcgccggtc gctgtgacgg tgtccccttc ttcgatgccg
     1381 gtgtagtccc ccaggaccat gacgccgacc gacgattctt cgaggttgag cgcgattccc
     1441 ttgacgccgt tttcaaacga taccatctca cccgacatca agccggagag accgtagacc
     1501 cgcgcaatgc cgtcccccac ctcgagaacc tgccccactt cgctcgtcga gacttcgggg
     1561 gaaaactgct cgatctcacg ttgaatgact gaactgatct cgtcggcttt gaatttcatg
     1621 catctttcct tcgatcagtc gttggcgaag ctgttcgagt cggctcttca ccgacgcgtc
     1681 a
//
LOCUS       DATAQL010000430         6195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3555605, whole genome shotgun sequence.
ACCESSION   DATAQL010000430 DATAQL010000000
VERSION     DATAQL010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555605"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VHS97_07450"
     CDS             complement(<1..531)
                     /locus_tag="VHS97_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448074.1"
                     /translation="MADSNVNPPLNRAGMAPRPGPGNSRSDLDDAADAALEKELAKRS
                     DDADVLDVPFKRQWDAGLEAELEAALAGFDAANFEVASPRAPRSDRVRSGDRDRGQEG
                     RPGARTGTVIGVRGKSVFVDLGGKSEGVISVTQFEEGKLPLAGASIEVIVDRFDPEEG
                     IQLLRLKGSAIEADWDN"
     gene            849..1559
                     /locus_tag="VHS97_07455"
     CDS             849..1559
                     /locus_tag="VHS97_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX3448075.1"
                     /translation="MPLPTLSTAELDSLRRYNTPTISNAIELFDVRPRHVGFLPHTIR
                     CLLPNLGPIVGYAVTSQTRAAPAEAGETRVDLLGDYLRYVASVPGPKIAVGWDRDTPP
                     GLGAQFGEVTATIHQKLGCVAHITSGCPRDLDEVEALGFQLFGLNPCVSHAHVRLLDF
                     GTPVMIGGVEIKSGDLIHADKHGVCLIPHEIAPKLAAACVEVESRERPLLEMCRGDNF
                     SLEEYIKLRAVKHDKIGE"
     gene            complement(1614..2084)
                     /locus_tag="VHS97_07460"
     CDS             complement(1614..2084)
                     /locus_tag="VHS97_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448076.1"
                     /translation="MGLLIGCGILSVCVIVAWMVLRRPLRQIVEDVHVDHARALFHQR
                     REWLEARFISALGRVDAVEGQRWETAQWHDEVLWARDRQTRCLLALVCVHFEPGPFEL
                     FQARRLATALFEFRKGQWCADGKSLDEMRPDEAVGRNPRFEAVTVTPPNPRRVG"
     gene            2217..3128
                     /locus_tag="VHS97_07465"
     CDS             2217..3128
                     /locus_tag="VHS97_07465"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX3448077.1"
                     /translation="MRRRQHRETPARKFGAHMSIAGGYDRAVRAAHAVNCRTVQLFTK
                     NNNQWNAPPISAEQVALFRQALDETGIVEPVAHTSYLINLGSPDDVLWQKSIDAMTVE
                     VERSSRLGIADLVVHPGSHMGQGEEAGLERIAQALDQVHARTEGLGVTIDLETTAGQG
                     TNLGYRFEHLQDILQRVAHPERLGMCADTCHIFAAGYSMGTPKEYDETIDRLDRSVGL
                     RNLRVWHLNDSCRECGSRVDRHAAIGAGQMGLEPFRHLVNDSRFRDLPMILETPKGVE
                     DGEDLDARNLKVLRQLVRRPRSSRTPK"
     gene            3241..5430
                     /gene="ppk1"
                     /locus_tag="VHS97_07470"
     CDS             3241..5430
                     /gene="ppk1"
                     /locus_tag="VHS97_07470"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX3448078.1"
                     /translation="MPTTQLNGQAVTAAAGAIVAPSVLEPGWFINRELSWLDFNERVL
                     EEAKDPSNPLLERLRFLAICASNLDEFFEVRVAGLQAQLYENLEPQDPPPDGMGPLAQ
                     LIEITRRAHDFVARMYETWHAEIRPLLVKHGIQICTPQDLTPAQVAFLDDYFDTQVYP
                     VLTPLAIDPAHPFPHVHNKSLNLLLRIEANKPHARPLYAVLQVPAAAVLSRLVPLPDE
                     GDGQHRFVLLEDVIGPRLDALFGGYRTTERVAFRVTRNSDLTIQENEAKSSLLSTIEE
                     TLRQRKWGAAVRLEISDRADEDFLAQLLSTSALELEDRDVYKVAGPVDLTALLALCKI
                     EGFRDLKEPPFEPQMPAPLAKRKSIFLAIRDQDILVHHPYESFGSVVQFLEQASEDPQ
                     VLAIKMTLYRTAEANPIITALARAAENQKQVTALVELQARLDEENNIDKARMLQKAGV
                     HVVYGIVGLKTHCKASLVVRRDHDGIRRYVHLATGNYNPTTARYYTDLSFFTCRPEFG
                     EDASVLFNLLTGYSPVNDWNKLIVAPAHLSSRLSALIERECAHAEAGRPARIIAKMNS
                     LVDPQTIALLYRASQAGVKIDLIIRGICCLRPGMPGISDNIRVISIVDKYLEHSRISY
                     FHNADEPEVFLSSADWMPRNFQRRVEIMFPIEDPQLKRRLVEGILGVVLADNVKAREL
                     QADGSYRRTAPAESGEPSIRSQLEFQNMALELCETSPISHATTAVGP"
     gene            complement(5960..>6195)
                     /locus_tag="VHS97_07475"
     CDS             complement(5960..>6195)
                     /locus_tag="VHS97_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448079.1"
                     /translation="RWLRERTDGRLMIVGHNPTLSDLVSLLVLGSTQPTICDLKKGGI
                     AALTRATADAELYEVHWIAPPWLIRGLMDGDNH"
BASE COUNT         1247 a         1974 c         1781 g         1193 t
ORIGIN      
        1 gttgtcccaa tcggcttcga tcgccgaccc cttgagacgg agaagctgaa tcccttcttc
       61 gggatcgaac cggtcgacga tcacctcgat ggacgccccc gcaagaggca atttgccttc
      121 ttcaaactgc gtgaccgaga tgaccccctc gcttttgcca cccaggtcga cgaaaacgct
      181 ttttccacgc acgccgatca ctgtgccggt gcgtgcaccg ggccggccct cttggccccg
      241 atcgcgatcg cctgagcgca cgcgatccga tctgggagca cggggcgacg ccacctcaaa
      301 gttcgccgcg tcaaagccgg caagggcggc ctcgagctcg gcttcaagcc cagcgtccca
      361 ctggcgcttg aacgggacgt cgagcacgtc ggcatcgtcc gagcgtttgg cgagttcctt
      421 ttcgagagcg gcgtcggccg cgtcgtccaa gtccgagcga ctgttgccgg gcccaggccg
      481 gggagccatg ccggctcgat tgagaggcgg attgacgttg gaatcggcca tggaatcaaa
      541 tccagcatga aatcgacggt attcgccagg atgaaaagac ttgggccggt cgcacaaccg
      601 tgatcgcttc gaaaccgaga ccgccaccaa gtagaatcac gaccctccag actatcgtgt
      661 tccgccggat gggccaagcc ggagatttct agccagacga cggcagtgtg cccactggtt
      721 ctggcagaat ccgttgactc accttcggtc cgcgggtccc tctgccctct tttctcgact
      781 caagacctgc gggtatgctg gccccaatcg ccgaaaaacg ttctgcatac gcgcagagga
      841 actcgatgat gccattaccg accttgagca ctgcggagct ggactcgttg cggcgataca
      901 acacgccgac gatctcgaat gccattgagc tcttcgatgt tcggccgcgg cacgtcggct
      961 ttttgcccca tacgattcgt tgcctccttc ccaacctggg cccgatcgtc ggctatgcgg
     1021 tgacttcgca gacccgtgcc gcaccggccg aggcggggga aacacgggtc gatctcttgg
     1081 gtgactacct gcgctacgtc gcctcggtgc caggcccgaa aatcgcggtc ggctgggatc
     1141 gcgatacacc tccaggcctc ggcgcccaat tcggcgaggt taccgcgacg atccatcaga
     1201 aacttggctg tgtcgcgcac atcaccagcg gttgcccgcg cgacctggac gaggtggagg
     1261 cgctcggatt ccagctcttc ggcctgaacc catgtgtgag ccatgcccac gttcgcttac
     1321 tcgatttcgg tacgccggtc atgatcggag gcgtcgagat caaaagcggc gatctgattc
     1381 acgccgataa gcacggcgtt tgcttgatac cccacgaaat cgcgcctaag ctggctgcgg
     1441 catgcgtcga agtcgagagc cgcgagcgcc ccctcctcga aatgtgccgc ggcgacaact
     1501 tctcgctcga agagtacatc aagttgcggg ccgtcaagca cgataagatc ggggaatgac
     1561 gtgcgatcaa cccgagcgca gccggccgcc gggtattccc accgccgtcg ggttcagccc
     1621 acccgtcggg gattgggggg cgtgacggtc accgcctcaa accgcggatt gcggccgacc
     1681 gcctcgtcgg ggcgcatctc gtccaagctc ttcccgtcgg cgcaccactg ccctttccgg
     1741 aactcaaaga gcgccgtcgc cagccttcgt gcttgaaaca attcgaacgg accgggctcg
     1801 aagtggacgc ataccagtgc cagcaggcac cgtgtctgac ggtcacgcgc ccagagaacc
     1861 tcgtcgtgcc actgggccgt ctcccaccgc tggccttcga ccgcgtcaac gcggccgaga
     1921 gcagagataa accgggcttc gagccattcc ctgcgctggt ggaataatgc acgcgcatgg
     1981 tcgacgtgca cgtcctcgac gatctggcgc agcggccgcc gcaagaccat ccaggccaca
     2041 atgacgcaaa cggacaggat gccgcacccg atcagcaacc ccatgttata ctcccgccac
     2101 ctccctgagt tggcaagcaa cgaattcatt gtagaatctc atcgtgtctg tccgcaagtg
     2161 acggacctcc ggcgtcatgc acgatgatga gggcttttac atcaatggtt cgaacggtgc
     2221 gccgtcgcca acaccgcgaa acccccgctc gcaagtttgg tgctcacatg tcgatcgccg
     2281 gcgggtatga ccgcgctgtt cgagcggcgc acgccgtgaa ttgccggacc gtccagctct
     2341 tcaccaagaa caacaaccag tggaacgccc cgccaatctc ggccgagcag gttgcgctct
     2401 ttcgacaggc cctggatgaa acgggaattg tcgagccggt tgctcacacg tcgtatctta
     2461 tcaatttggg aagtcccgac gacgtcctgt ggcaaaagtc gatcgacgcc atgacggttg
     2521 aagtcgagcg ctccagtcga ctcggcatcg ccgacttggt ggtccatccc gggtcgcaca
     2581 tgggccaggg ggaggaagcc ggcttggagc gaatcgccca ggctctcgat caggttcacg
     2641 cacgcaccga aggcctggga gtcacgatcg acctggaaac gaccgccggc caggggacca
     2701 acctgggcta tcgcttcgag catctgcagg atattctgca gcgcgttgca catcccgagc
     2761 gtctcggcat gtgcgcggac acctgccata ttttcgcggc aggctattcc atggggacgc
     2821 cgaaggagta cgatgaaacg atcgaccggc tcgaccggtc cgttgggctg cgaaacctgc
     2881 gcgtctggca tttgaacgac agctgccgtg aatgcggcag ccgcgtcgat cgccacgcgg
     2941 cgatcggggc ggggcaaatg gggctcgaac cgtttcggca tctggttaac gattcccgct
     3001 tccgggatct accgatgatc ctggaaacac caaagggtgt cgaagacggg gaagacctcg
     3061 atgctcgcaa cctgaaagtt ctgcgccagc tcgttcgtcg gccgcgttct tcccgcacgc
     3121 ccaagtgagt tcatgcggat tgaacgtttc tttcgagcct tccgcgagcc gtctcgcttc
     3181 cctgtttcgg gccggacctt atcgggggac gaaggtgaga ccatggcgga gcgtatggtc
     3241 atgcccacaa ctcaactaaa cggtcaggcg gtgacggctg cggcaggggc catcgtcgcg
     3301 ccctcggtct tggagccggg ctggttcatc aatcgcgagc tctcctggct cgattttaat
     3361 gaacgggtct tggaggaagc aaaggaccca tctaacccac ttctcgagcg gctacgattt
     3421 ctggcgatct gtgcgtcgaa ccttgacgag tttttcgagg tacgcgtcgc cgggctccag
     3481 gctcagctct atgaaaacct cgagccgcaa gacccacccc ccgatggcat ggggcccttg
     3541 gcacaactga tcgagatcac gcgtcgtgcg catgatttcg tcgcccgaat gtatgagacg
     3601 tggcacgccg agatccgtcc gctcttggtc aaacacggca tccagatttg caccccgcag
     3661 gacctgacgc ccgcccaggt cgcgtttctc gacgactatt tcgacaccca ggtctatccg
     3721 gtgttgactc cgctggccat cgacccggcc cacccgtttc cgcatgtgca taacaagagc
     3781 ttgaaccttc tcttgcgaat cgaggctaac aagccgcacg cacggcctct ctacgccgta
     3841 ctccaggttc cggcggctgc ggtcttgagc cgcctggttc cactgccgga tgaaggggat
     3901 ggccagcaca ggtttgtctt gctcgaagac gtcattggcc cgcggttgga cgctctcttc
     3961 ggcgggtacc gcacgaccga gcgggtcgcc tttcgcgtga cgcgcaatag tgacctgacc
     4021 atccaggaaa acgaggccaa gagcagcctg ctctccacga tcgaggaaac cctgcgtcaa
     4081 cgcaaatggg gagcggccgt gcggctggag atatcggacc gagccgatga agactttctg
     4141 gctcaattgc tttcaacctc ggcactcgag ctcgaagatc gcgatgtcta taaggttgcc
     4201 gggccggtcg acctgacggc gctcttggcg ctttgcaaga tcgaggggtt ccgggacttg
     4261 aaggaaccgc cattcgagcc gcaaatgccc gccccactgg ccaagcgcaa aagtatcttc
     4321 ctcgccattc gcgaccagga catcctggtg caccacccgt acgaatcgtt cggctcggtg
     4381 gttcagttcc ttgagcaggc gtcggaagat ccgcaggtcc tggcgatcaa gatgaccctt
     4441 tatcgcacgg ctgaagccaa tccgatcatc acggcgctag cccgcgccgc cgaaaaccag
     4501 aaacaggtga cagcgctcgt ggagctccag gctcggctcg acgaggaaaa caacatcgac
     4561 aaggcccgta tgcttcagaa agctggcgta cacgtcgtat acgggattgt cggtctcaaa
     4621 acgcactgca aggcatcgct ggtcgtaagg cgcgatcacg acggcatccg cagatacgtt
     4681 caccttgcta ctggcaatta caatcccacc acggcgcgat actacaccga cctcagtttc
     4741 ttcacctgtc gacccgagtt cggcgaagac gccagcgtgc tctttaacct cttaaccggg
     4801 tactcaccag tcaacgactg gaacaagctg attgtcgctc ccgcgcacct ttcgagccgg
     4861 ttaagcgccc tgatcgagcg cgagtgcgca cacgcagagg ccggcagacc cgcgcggatc
     4921 atcgccaaga tgaattcact ggtcgacccg cagacgatcg ctttgctcta ccgagcctcc
     4981 caggcgggag tcaagatcga cctcatcatc cgcggaatct gctgcctgcg gccggggatg
     5041 ccgggcatat cagacaatat tcgggtcatc agcatcgtcg acaaatacct cgaacattcg
     5101 cggatctcgt acttccacaa tgccgatgag ccggaagtgt ttctctcatc ggccgactgg
     5161 atgccgcgca actttcagcg ccgcgtcgag atcatgttcc cgatcgaaga ccctcagctc
     5221 aagcggcggc tggtcgaggg gattctcggg gtggtgctgg ccgacaacgt caaggcccgc
     5281 gagctccaag ccgacggaag ctaccggcgg accgcgcccg ccgaatctgg tgagccgagc
     5341 attcgctcgc agctcgaatt tcaaaacatg gcgctggagc tgtgcgagac cagccccatc
     5401 agccatgcaa cgaccgccgt ggggccgtga taccaccgcg cctgggacag ccgtacgcga
     5461 accacggacc ggtcatccgg cgcgactctc cgttggttgg cgtcagttcg cacttcaatc
     5521 aggatagaaa ccgatctaac cacgaaatag acgaaataca cgaaaaagat taacgatatt
     5581 ataagatgat acgttcatat tcgacttggg ggtgagcact caagttgacg accaggtcga
     5641 gccgcatgcc agccctaagc ttcttgatcg taaatcagct cggccatgtc accacacccg
     5701 gacctcttaa tcgtcttttt tcgtgtattt cgttcttttc gtggttaatc cgatcgtctc
     5761 aaccgcgata gcgagccgtc aatccagagg ccgacctcac gccggacgag cgcctcggct
     5821 tgctcgtcta aggatttgca cctgctccca ttcgccgggt tcatatcggg cctggcctgg
     5881 atctgtggtt cgcgatacca tttctccagg tcgttggtag acacgcacta agccggtcaa
     5941 aattataaat aagtcaacat tagtggttgt ccccatccat cagcccccga atcagccacg
     6001 gcggcgcgat ccagtgcact tcgtacagct cggcatcggc cgtggcccgc gtcaaggcgg
     6061 caatgccacc ttttttcagg tcgcatatgg tcggctgggt cgatcccaga acaagaagcg
     6121 acaccaggtc gctaagggtc gggttatgac cgacgatcat cagccggccg tccgtgcgct
     6181 cacgcagcca gcgct
//
LOCUS       DATAQL010000431         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_195327, whole genome shotgun sequence.
ACCESSION   DATAQL010000431 DATAQL010000000
VERSION     DATAQL010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195327"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..2184
                     /locus_tag="VHS97_07480"
     CDS             157..2184
                     /locus_tag="VHS97_07480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX3448080.1"
                     /translation="MRVLVGCSLALIAAERGAQAADGLAPRSGVDATGFNKGVRPEDD
                     FFRFVNGGWIERTEIPADRSIYGSFITLRDKSESNLRAILEDSAAKSGESNGSEARKL
                     GDLYASFMDEARIEELDKKPVESDLARIDALADKAALTRTAAALQREGVSGLFGALVT
                     NDAKKSDRYIVYLNQGGISLPDESYYRLPKFQPIRDKFVAHVERMFALAGIPEPKAAA
                     ARVMAVETELAKHHWDRVKSRDRTLTYNKFDSAGLEALTPGYDWSAWWKEFAAKPIDE
                     VVVRQPDYFKAMANLLDSVPLADWKTWLKWQVLLDAAPLLSKPYVQENFDFYDKTLTG
                     TPEMRPRWKRGVGVVERTMGEAAGKIYVSKHFPPDAKSRMKDLVGNLIAAYRENIADL
                     EWMSPETRKRALEKLSKFNPKIGYPDKWRDYQALEIHRDDLVGNMRRAAAFETAYRFN
                     KLGKPVDRNEWGMTPQTVNAYYNSSMNEIVFPAAILQPPFFDMEVDAAVNYGAIGAVI
                     GHEIGHGFDDQGSKFDGDGNMINWWTDPDRQEFEKRAKMLIDQYSEFEPAQLPGQKVN
                     GALTIGENIGDLGGLAIALKAYRRSLQGRESAVIDGLTGDQRFFIGWGQAWRSKARDA
                     EVARRLATDPHSPAEFRCNGVLRNMPEFYEAFGVKNGDRLWLPAERRVRIW"
     gene            2212..3219
                     /locus_tag="VHS97_07485"
     CDS             2212..3219
                     /locus_tag="VHS97_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX3448081.1"
                     /translation="MDDRSIPETLDRMVAGYFVSQAIYVAAELGIADRLAEGPRRAEE
                     IARDVGAHERSLYRLLRALASVGVFAEDADGRFSLTPLADLLRSDVPGSQRATIQMMV
                     GQFYDAWRGLLETVRTGRSSFEKLHGRPFFESLADDPIQAQIFDDAMTARNDRKTQAM
                     LVAYDLTGVRVLADVGGGNGSTLVTVLKQYPEMQGILFDQPGVVERARGRIEREGLTG
                     RCQLVPGSFLESVPSGAGAYLLRHILHNWDDEHVVEILSRVREAMGKGARLLIVDRII
                     TRGNEPLFGKLMDLNMLVMLGGVERTEEEFRDLFRRAGFRLSRIVATEAEVSVIEGAK
                     D"
     gene            complement(3372..>3564)
                     /locus_tag="VHS97_07490"
     CDS             complement(3372..>3564)
                     /locus_tag="VHS97_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448082.1"
                     /translation="DGPWRYVRPDRRKEKAHLAGYDPAGAPAFEWEPALREARTKHYE
                     KVKRDSAKERVGDASKSKE"
BASE COUNT          716 a         1050 c         1100 g          698 t
ORIGIN      
        1 cgatcctggt tagattggct cgtctcagcg ggacttgccc tagcgaccgg gaccaaccgg
       61 cggcgctgcg acaactttat tcggtcgagg ccaactgctc cgtcttccat caccttcacc
      121 tttgaactga taggatatga aatgagatat atgtggatgc gagttcttgt tggctgctcg
      181 ctcgctctga tcgccgccga gcgcggtgct caagcggcgg atgggttggc gccccgatcg
      241 ggggtcgatg caaccggttt taacaagggt gtgcggccgg aggatgactt ttttcgattc
      301 gtcaacggcg gctggatcga gcggacagag atacctgccg atcgctcgat ctatggctcg
      361 tttatcacgc tgcgagacaa gagcgaatct aatctgcgtg cgatcctcga ggattccgca
      421 gcgaagtcgg gcgagtcaaa cggttccgag gcgcgcaagc tgggagacct ctacgccagc
      481 ttcatggatg aggcccgcat cgaagagctc gataagaagc cggtcgagtc cgatctcgcc
      541 cggatcgacg ccctggccga caaggccgcg ctgacgcgca ccgcggcggc actgcagcgc
      601 gagggagtgt cggggctttt tggggcgctc gtcaccaacg acgccaaaaa gtcggaccgc
      661 tacatagtct acctgaatca aggtggaatc agtctccctg acgagtccta ttaccgcctt
      721 cctaagttcc agcccattcg cgacaagttt gtcgcccacg tcgagagaat gttcgccctg
      781 gcgggtatac ccgagcccaa ggccgcggcc gctcgggtga tggctgtcga gaccgagctg
      841 gccaagcatc actgggatcg ggtcaagagc cgcgatcgaa cgctcacata caacaagttc
      901 gatagcgccg ggctcgaggc attgaccccc ggctatgact ggtccgcgtg gtggaaggaa
      961 ttcgcagcca agccgatcga cgaggtcgtg gtccgtcagc cggactattt caaggcgatg
     1021 gcaaacttgc tcgacagcgt tccgctcgcc gattggaaga catggctcaa gtggcaagtg
     1081 ctgctcgacg ccgcgccgct tttgagcaag ccttatgtcc aggaaaactt cgatttttac
     1141 gacaagactc tgaccggaac acccgaaatg cgcccgcgct ggaagagggg cgtgggagtg
     1201 gtcgagcgga ccatgggtga ggcggccggc aagatttatg tgagcaagca cttcccgccc
     1261 gacgccaagt cacgaatgaa ggatctggtg ggcaacctga tcgcagccta tcgcgaaaac
     1321 atcgccgatc tggagtggat gagccccgaa acccgcaagc gggctctcga aaagctcagc
     1381 aagttcaacc ccaagatcgg ctatcccgac aagtggcggg actatcaggc gctcgagatc
     1441 caccgtgatg acctcgtcgg caacatgcgg cgggcagcgg ccttcgagac ggcgtatcgg
     1501 ttcaacaagc tcggcaagcc ggtcgatcgc aacgaatggg gcatgacacc acagaccgtc
     1561 aacgcatatt acaattccag catgaacgag attgtcttcc cagcggcgat cttgcagccg
     1621 ccgttcttcg acatggaagt cgacgccgcc gtcaactacg gcgcgatcgg cgcggtcatc
     1681 ggccatgaga tcggtcacgg cttcgacgac cagggatcga agttcgacgg cgacggcaac
     1741 atgatcaact ggtggaccga cccggaccgc caggagtttg aaaagcgggc gaaaatgctg
     1801 atcgaccagt acagcgagtt cgaaccagcg caacttccgg gccaaaaggt caatggggcg
     1861 ctgactattg gcgaaaacat cggcgacctg gggggcctcg ccatcgctct caaggcctat
     1921 cggcggtcgc tgcaaggccg tgagtccgcc gtgatcgacg ggctgaccgg cgaccaacga
     1981 ttcttcatcg gctggggcca ggcgtggcgc agcaaggcac gtgacgccga agtggcgcgg
     2041 cgtcttgcca ccgatcctca ttctccggcc gaatttcgct gtaacggagt ccttcgcaat
     2101 atgcccgaat tctacgaggc cttcggcgtc aaaaacggtg acaggctctg gttgccagcc
     2161 gaacgacgcg tccgtatttg gtaagcgggc accttgctgg aggaacgtcg tttggacgat
     2221 cggtcaattc ctgagacact tgaccggatg gtcgccggct attttgtctc gcaggcgatc
     2281 tacgtcgccg ccgagttggg gatcgccgac cgactcgcgg agggtcctcg tcgcgccgag
     2341 gagatcgcgc gcgacgtagg agcccacgaa cgctcccttt atcggttgct tcgtgcgctg
     2401 gcaagcgtcg gcgtttttgc cgaagatgcc gacgggcgtt tctcactcac gccactcgcc
     2461 gatttgctgc ggagcgacgt tccgggctcg cagcgagcga cgattcagat gatggtcggc
     2521 cagttttatg atgcctggag aggtttgctc gagaccgtcc gcaccggtcg gtcttcattt
     2581 gaaaagctgc acggccgacc atttttcgag tctctcgccg atgacccgat tcaggcccag
     2641 atcttcgacg acgcaatgac ggcccgaaac gaccggaaga cacaggccat gctggtggcg
     2701 tacgacctta cgggcgtccg agtcctggcc gacgtcggcg gcggtaatgg cagcactctc
     2761 gtgactgttc tcaagcagta tcccgaaatg caaggcatcc tgttcgatca gcccggtgtg
     2821 gtcgagcgtg cccgcggtag gatcgagcgc gagggattga ccggccgatg ccagttagtg
     2881 cccggcagct ttctcgaaag tgtaccaagc ggagcaggcg cctacctgct ccgccacatc
     2941 ctccacaact gggacgatga acacgtggtt gagatcctca gtcgcgttcg cgaagcaatg
     3001 ggcaaagggg cgaggctttt gatcgtcgac cggatcatca cgcgcgggaa tgaaccgttg
     3061 ttcggcaaac tcatggacct gaacatgctc gtcatgctcg gcggagtcga gcgaaccgaa
     3121 gaggagtttc gcgacctctt tagacgtgcg ggatttcgcc tgagccgcat cgtggcaacc
     3181 gaggccgaag tcagcgtgat cgagggagcg aaagactgat cgaccgacgg ccggacactc
     3241 gcggtcttcg tattgaaatc ccgtctgccg gccggcctgc ttcgacaagt aatccgactt
     3301 gtacgaatcg gttttccacc acgttctgga ggcgatatct tcggcaacct gccgtatctg
     3361 gacttccgat cttactcctt gctcttggag gcgtccccga cgcgctcttt tgcgctgtct
     3421 cgcttcactt tttcgtagtg cttggtgcga gcctcacgta gtgcgggctc ccactcgaat
     3481 gccggcgcac cggctggatc atatccggcc aggtgagcct tttccttgcg gcgatcgggg
     3541 cggacatagc gccaagggcc gtca
//
LOCUS       DATAQL010000432         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2639040, whole genome shotgun sequence.
ACCESSION   DATAQL010000432 DATAQL010000000
VERSION     DATAQL010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2639040"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VHS97_07495"
     CDS             <1..1013
                     /locus_tag="VHS97_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX3448083.1"
                     /translation="FIAGGTGLIGRGLAKALLDSGHQPIILSRNADSVRRDREMWPYQ
                     VVAGDPTKPGRWQDEVDGCDAVVNLAGHSIFADRWNAGIKRKIRDSRVFSAEHLVHAI
                     KNAHARPKVYVQGSAVGFYGPEGDQELTESSPSGSDFLAVVCRECEDVSREFDQLGVR
                     RAIVRTGIVLAANAGALKIMTPIFKLGPGTPIGSGGGLVAKGDQWMSWIHLDDIVGIF
                     KLALEKDNAAGPLNGTAPHPVRNAEFAKTFTGVLKKPFTPWRIYLPFGPPDGLLRLVL
                     GDVATVVTTGQKVLPAKPHELGYVFKYPYLADALRAIFTAPPAQAPAVAHHHPAGAGA
                     HH"
     gene            complement(1029..2339)
                     /locus_tag="VHS97_07500"
     CDS             complement(1029..2339)
                     /locus_tag="VHS97_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3448084.1"
                     /translation="MASFEKPKAADGAPLDLLPVIRSSGIVNERLLAEIKSKVLQGDY
                     PLDSAALAERLVRDNILTSFQAKRLLSNKPQGLIVGRYIVLDRIGSGSMGRVYRAHHV
                     MMDRVVALKIIAPEIAQNERVVARFQREMKLVGRLDHPNVVRAFDADQINRILYIVME
                     YVNGMSLGERLKKKGPIPPAEMVDYAAQAALGLAHAHEQGIVHRDIKPSNILITDDRR
                     VKILDLGLGVLMEADSAATFATADGIAVGTVDYMSPEQACGREVDGRSDLYGLGCSMY
                     HLMSGRLPFPGSTPIERLGKRIGGRHTPITEYLPDVPASFVRVLDKMLAHKPHERYAT
                     AAAAADALQNLIRPKAKAPTPVQQLVAQINVSAGNPPAEAKPAPEMQEPQGPVSIKVR
                     PIYPAWFARAAGFVERRPRTALAWVIVTLAATFAAGIGLGIAIK"
     gene            2885..>3719
                     /locus_tag="VHS97_07505"
     CDS             2885..>3719
                     /locus_tag="VHS97_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3448085.1"
                     /translation="MEKNGVMDAVFRDLQTLFDMGVMGALSDGQLLDRFVERREEAVF
                     EVIVRRHGPMVWGVCRRVLRDHHDAEDAFQATFIVLARKAASVTPREKLGNWLYGVAY
                     QTAMKAKAMRAKRRVREVHVPDMPEPMAVPEDLRDELAESLDRELIRLPEKYRIPIVL
                     CELEGKTHNEAAEQLGWPIGTVSSRLSRAKAMLARRLSRLGVSLSAGSLAVLLAQESA
                     SASMPTSLIGSTTKAASLFAAGGAVAAGVVSTGVAALTREVLKMMLLGKLKLGVVMLL
                     VT"
BASE COUNT          693 a         1147 c         1186 g          693 t
ORIGIN      
        1 ttttcatcgc ggggggaacg ggtctgattg gccgcggcct ggcaaaggca ctcctggatt
       61 caggccatca gccgataatc ctgtcccgga atgccgacag cgtgcggcga gaccgcgaaa
      121 tgtggcccta ccaggtcgtc gccggcgacc ccaccaagcc cggacgatgg caggatgagg
      181 tcgatggatg cgacgccgtc gttaatttgg cagggcacag catcttcgcc gaccgctgga
      241 atgccgggat caagcgcaag attcgcgaca gccgcgtttt tagcgccgag cacctggtcc
      301 atgcgatcaa gaacgcccat gctcgtccca aggtctacgt gcaggggtcg gccgtcggct
      361 tctacggccc cgagggggac caagagctca ccgagtcgag cccatccggg tccgactttc
      421 tcgcggtcgt ctgccgtgaa tgcgaggacg tctcgcgtga gttcgatcaa cttggcgtcc
      481 gccgggccat cgtccggaca gggatcgttc tggccgcgaa cgccggcgca ctcaagatca
      541 tgaccccgat cttcaagctc ggcccgggta ctccaatcgg cagcggcggc ggactcgtag
      601 ccaagggaga tcagtggatg agctggatcc acctcgatga catcgtcgga atcttcaaat
      661 tggccctgga aaaagacaat gccgccggac ctttgaatgg gacggcgccc catccagtcc
      721 gcaacgcgga attcgccaag acctttaccg gggtactcaa gaagccgttc accccatggc
      781 gaatttatct ccctttcggc cctcccgacg ggctgctgag gctcgtgctc ggcgacgtgg
      841 ccacggtcgt gacgaccgga caaaaggtcc tgcccgcgaa accgcatgaa ctgggttacg
      901 tcttcaagta tccctacctg gccgacgccc tccgtgcgat cttcacggca cctccggccc
      961 aagcacccgc ggtcgcgcac catcatccgg ccggtgcggg cgcccatcat tgaggcggtt
     1021 ctccccggct acttgatggc gatccccagg ccgattcccg cggcgaatgt cgccgccagt
     1081 gtgacgatga cccaggccag ggcagttctc ggtctccgct cgacaaatcc ggccgcccgt
     1141 gcgaaccaag ccggatagat gggccggacc ttgatggaca ctgggccttg cggttcttgc
     1201 atctcggggg ccggcttggc ttccgcgggt ggattgcctg ccgagacgtt gatctgggca
     1261 acaagctgtt gaaccggtgt cggcgccttc gccttgggtc ggatcaggtt ttggagcgca
     1321 tcggccgcag cggccgccgt cgcatatcgt tcgtgcggct tgtgagccag catcttgtcg
     1381 agcacacgga cgaagctggc cggtacgtcc ggcagatatt ccgtgatcgg ggtgtgccgg
     1441 ccgccaatcc gcttgcccag tcgctcgatc ggcgtactgc cgggaaacgg tagcctgccg
     1501 ctcatcagat ggtacatgga acagcccagg ccatataaat cgctgcggcc gtcgacctcg
     1561 cgcccgcacg cctgctcggg ggacatgtaa tcgaccgtgc cgacggcgat gccatcggcc
     1621 gtcgcaaacg tggcggcact gtcggcttcc atcagcacgc ccagcccgag gtcgaggatc
     1681 ttgacccggc gatcatccgt aatcaggatg ttgctcggct tgatatcgcg atgcacaatt
     1741 ccctgttcgt gggcatgagc caatcccagc gcggcctggg cggcatagtc aaccatctcg
     1801 gcggggggaa tcggtccctt tttcttgagg cgctccccca gactcatgcc gtttacatac
     1861 tccatgacga tgtacaagat cctgttgatc tggtcggcgt cgaaggcgcg aaccacgttg
     1921 ggatgatcga ggcggccgac cagcttcatc tcgcgctgaa atcgcgccac cacgcgttcg
     1981 ttttgcgcga tttcgggtgc gatgattttg agcgcaacca cccgatccat catgacgtga
     2041 tgcgcccgat agactcgccc catcgagccc gaaccgatcc ggtcgagaac gatgtatcgg
     2101 ccgacgatca agccctgcgg cttgttgctc aaaagccgct tggcctgaaa cgaagtcaag
     2161 atattgtcgc gcaccaggcg ctcggcaagc gctgccgaat cgagcggata atcaccctgg
     2221 aggaccttgc tcttgatttc ggcgagcagg cgttcgttga cgatgcccga actgcgaatc
     2281 accggcagca aatcgagcgg cgctccatcc gccgccttcg gtttttcgaa gctcgccatc
     2341 gcgtggctct ccgactcgct cgcgaccata attcgcgcaa tcgccagttt tcacaaatca
     2401 tggcgttata acaagatgcc gagatcccta cgcacttggt gcatcgccgt gaccacgacg
     2461 ataccacatc gcaggacgac gctcaatcgc cagtgtaggg cggcttacat gattggccgt
     2521 ccgatcgatg gattcgatca agacgagccg cctgcgctct ggtgactcag tttctctcac
     2581 gccgctcggg ttggcagatg cgtgattgtt tcctagctcg aactaccatc gtgacggacc
     2641 cgttcaagag cgtgaagttg taaacgtcga tttctccgcg aaaatgaacg ttgggccgat
     2701 cgacgacttg ccacccagtt gcctcgaact cgacggcacg ccaggcggaa tcgcccaggt
     2761 ggaggaagag cgatcacgac acttgcgacg gaaacgacga gcgaaattaa gaaaagcgga
     2821 tgagaacgag ctcgaccgtg gaataagcgg ttagaggacg agcgagatct gcggaagggg
     2881 cgacgtggag aaaaatggcg tgatggatgc ggtgttccgt gacctccaga cccttttcga
     2941 catgggggtg atgggggctc tctcggacgg gcaactcctg gatcgcttcg tggaacggcg
     3001 ggaggaggcc gtcttcgagg tgatcgtccg tcgccacggc ccgatggtct ggggcgtctg
     3061 ccgccgggtc ctccgggacc accacgacgc cgaggacgcc ttccaggcca ccttcatcgt
     3121 ccttgcccgc aaggcggctt cggtcacgcc acgggagaag ctcggcaact ggctctacgg
     3181 cgtggcgtac cagacggcca tgaaagcgaa ggcgatgagg gccaagcggc gggtgcggga
     3241 agtccacgtt ccggacatgc cggagccgat ggcggtgccc gaggacctgc gggacgaact
     3301 ggccgagtcc ctcgatcggg agttgatccg gttgcccgag aagtaccgca tccccatcgt
     3361 cctctgcgag ttggagggga agacccacaa cgaggccgcc gaacaactcg gctggcccat
     3421 cgggacggta tcgagccgcc tgtcgagggc caaggcgatg ctggcgaggc ggctgtcccg
     3481 cctgggcgta tcgctgtcgg cgggatcgct ggcggtgctg ctggctcagg agtcggcgtc
     3541 ggccagcatg ccgacgagtt tgatcggttc cacgacgaag gccgcaagcc tgtttgcggc
     3601 ggggggagcg gtggcggcgg gggtggtctc gaccggggtc gccgccctga cgagggaggt
     3661 gttgaagatg atgctactcg gcaagctcaa gctcggggtg gtgatgctcc tggtgacct
//
LOCUS       DATAQL010000433         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4928528, whole genome shotgun sequence.
ACCESSION   DATAQL010000433 DATAQL010000000
VERSION     DATAQL010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4928528"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..745
                     /locus_tag="VHS97_07510"
     CDS             167..745
                     /locus_tag="VHS97_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448086.1"
                     /translation="MQSLRSIKNDAEPARADAARGANESPEPFATNRGAIMNDSRQPA
                     IVDPRAGIAQPLPGTQPTSNVIPPPLHYSGNVDLSNRNNNPPDPRELEFPLPPVIGGF
                     KALTPAAGNVPQKEARKTSQSAGKTDPSLTANSQSAAAASTKSTSAVDAAKSAGQAKA
                     TDQAGEPKKKSKPLTIDPNILERALLNRNNGR"
     gene            1404..>1691
                     /locus_tag="VHS97_07515"
     CDS             1404..>1691
                     /locus_tag="VHS97_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448087.1"
                     /translation="MTRLFGRTRRLTYGPVLLLLALVAGCGEDKPQKPKVQARDTLGK
                     TTQEVRNLKPELAQGGQVTDGKIHATDYITLQADAYRTSVANIAKMKVKYDM"
BASE COUNT          380 a          564 c          441 g          306 t
ORIGIN      
        1 cgcgctgcgg gccaatctcg acgccaccct gcggctcatc gaccccgaaa gccccgccaa
       61 gagcgaacta ctctccagca cgctccgagc ccacggcacc ggcccacgcc cacagccgat
      121 cttcaccggg tagaacgaca ggagctacca ggctatcgcc gcatgggtgc agagccttcg
      181 tagcatcaag aacgacgccg agcccgcacg cgcggatgcg gcgcggggag cgaacgagag
      241 tcccgagccg tttgccacca accgcggcgc gattatgaac gattcgcgcc aacctgcgat
      301 cgtcgacccc agggccggca tcgcccagcc attaccggga actcagccga cgtcgaacgt
      361 cataccgcca ccacttcact acagcggcaa cgtggacctc tccaaccgca ataataaccc
      421 tcccgaccca cgggaattag agttcccgct acctcccgta atcggcggat tcaaggcgct
      481 gacgcccgca gctgggaacg ttccccagaa agaagcgcgc aagacgtccc agtccgctgg
      541 gaagacggat ccatcattga cggcaaattc acagagcgcg gccgcggcct cgaccaagtc
      601 tacatctgcg gtagatgccg ccaagtctgc gggccaggcc aaggcaaccg atcaagccgg
      661 agagccgaaa aagaagagca agccgctgac gatcgacccc aacattctgg aacgggcgct
      721 tctgaaccgc aacaacggcc gctaaggtcc gcttgacctg tcatcgtttc ggcggccgga
      781 cgacaccgga gtcgatccac tgaaggacgt aacgttgatt agctcttctt tattttatga
      841 tttttggtta ttttaaattg gcttggtcgg acgatcgaca tagttcatcg cgattgatct
      901 cgagtcgttt accggcttgt ccatctcgct tttcacgccg ccggcgctcc ttggtttgtt
      961 cccttaaaac gaccaacgac ccgaatgacc gagaccgcga tccatcgccc gcacgaactc
     1021 tttccgacct tttcgcactt tcctgagttg ccacgatgtt tcccaaatac agacactcac
     1081 cactgatcga tcccgaccgg aaacgctgtc cggtatgcaa tctgcccgtt tattcagcga
     1141 ccggcatcca tccgcaatgt gccctcaatc gagccgttgc gcttgagaaa aaaatcaaga
     1201 aagagtcaga ctcgctggcg gataccgcca ttgctttggc gcgtgtcgcg actggcgacg
     1261 ggacgaaaac gccgatcgcg gcatcctgag cccaggttcg ttcacacaac caacctgctg
     1321 tgccttgcac tgcttacctg atgacctgat actcgaatca aggctggctt gcggtctcaa
     1381 tcgtctcacc caggactcaa ctcatgaccc gactctttgg ccggaccagg cgcctgactt
     1441 acggaccggt cctgctcctg ctcgcgctcg tcgccggctg cggcgaggac aagccgcaga
     1501 agccgaaggt gcaggcgcgt gatacgctcg gcaagaccac tcaagaggtg aggaacttga
     1561 agcccgagct cgcccagggc ggtcaagtca ccgacggcaa gattcacgcc accgactaca
     1621 tcacccttca ggccgatgcg taccgcacct ctgttgccaa cattgccaag atgaaggtca
     1681 agtacgacat g
//
LOCUS       DATAQL010000434         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3401105, whole genome shotgun sequence.
ACCESSION   DATAQL010000434 DATAQL010000000
VERSION     DATAQL010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3401105"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VHS97_07520"
     CDS             complement(<1..1229)
                     /locus_tag="VHS97_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448088.1"
                     /translation="MLRKLPSVALLALMGFGLSVTAHPAQGASYAGGASYRICPVPQE
                     CSGHTQYQLQRQTVLQNVQETVYDSQQVPVVRTVYETVLQPKTITTVRNVVEQQCRDE
                     PYTVQRPTYRTVPREVHYTVQRPVTRTVWKDVAYTVTRPIRETHFETQSYTVSHPVRE
                     TSFKTVPYNVSHSVREVSYKDVTYTVCRPIQETYYKTVSYTVCRPVQETRYKTCAYTI
                     CRPVQSVRYRCVPYTVQVPVQQTIMQCQAATEMVPVKSCAYKQVPYTVSRPIRETHLQ
                     ECRYTVHVPVKRTELRSTTYTVCRPVRETHLQECRYTVCKPVQTTTYKTVSKTCYQTI
                     TETAYRESCETVCVPKTVMKPVCRTYNEAVVQRYYVPGKTVCINGCLVQCPGTWCDKT
                     VCCPRTVTETVACTVYES"
     gene            complement(1692..2255)
                     /locus_tag="VHS97_07525"
     CDS             complement(1692..2255)
                     /locus_tag="VHS97_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448089.1"
                     /translation="MRRGYSIRVSEDDWDSPEVRLGWARLIAGSLDSDLLGKCPEFVD
                     HLRSTNDPSRFYLATVRDAAGSICGVVPLCIAPSGLKFEISGKVLAESRSPAVRILGS
                     VPLLPADPVAHDLLFATLHQGFSGCQAISMASVPTNSFLWDYVRESGESTWALGRRLI
                     PIPPPTPPSHVPVCSCSAGHCPIDSAG"
     gene            2687..3463
                     /locus_tag="VHS97_07530"
     CDS             2687..3463
                     /locus_tag="VHS97_07530"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX3448090.1"
                     /translation="MSGSVLDRFRLDGKVALVTGGARGLGRVIADALASAGADVAITA
                     RQAESAQGAAELIAAMSTRKAIGLAADVTQRSSVEALVAHVLETLGRLDILVNNAGVN
                     IRGPIEELSEDDWDTVIDTNLKGPWLCCRAVAPVMKRQKSGRVINVSSMLGEISMPGR
                     SPYASSKGGLTLLTKTLALEWAKDGINVNALCPGPFATEINTPLLNDPAARAQMEANV
                     PLARWGDPVELGPAAVFLASEASSFMTGATLFIDGGYTAR"
     gene            3550..>3919
                     /locus_tag="VHS97_07535"
     CDS             3550..>3919
                     /locus_tag="VHS97_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX3448091.1"
                     /translation="MSDSAQLAIPRGFRASGVKAGLKASGSLDLAVLAADQPCAAAGT
                     FTTNRVCAAPVKWCRELLPADNIRAIAINAGNANAATGEQGLANARRTAERVARLLGC
                     QPEEVLVASTGIIGHQLAMDK"
BASE COUNT          802 a         1145 c         1206 g          766 t
ORIGIN      
        1 gactcgtaga ccgtgcaggc gaccgtctcg gtgacggtcc gagggcagca gaccgtcttg
       61 tcgcaccagg tgccggggca ctggaccaga cagccgttga tgcacacggt cttgccgggc
      121 acgtaatacc gctgcacgac cgcttcgttg taggtacggc agacgggctt catgaccgtc
      181 ttgggcacgc acacggtctc gcacgactca cgataggcgg tctcggtgat tgtctgatag
      241 cacgtcttgc tgaccgtctt gtacgtcgtc gtctggacgg gtttgcaaac ggtgtagcgg
      301 cactcctgga ggtgcgtctc acgcacgggg cggcagacag tgtaggtcgt cgaccgcagc
      361 tcagtgcgct ttacgggcac atggaccgtg tagcgacatt cctgcaggtg ggtctcgcgg
      421 atcggccggc tgactgtgta cggaacttgc ttgtaagcgc acgatttcac cggcaccatc
      481 tcagtcgcgg cctggcactg catgatggtt tgctgcaccg gcacctgaac cgtgtagggt
      541 acgcagcggt agcggaccga ttgcaccggc cggcaaatcg tgtaggcgca ggtcttgtac
      601 cgcgtctcct gcaccggccg gcagacagtg tagctgaccg tcttatagta agtttcctgg
      661 atcggccggc agacagtgta agtgacgtcc ttgtatgaca cctcgcgaac cgagtggctc
      721 acattgtacg ggaccgtctt gaacgaggtt tcccgaaccg gatgagagac tgtgtacgac
      781 tgcgtctcga agtgtgtttc ccgaattggt cgggtaaccg tgtaggcgac gtctttccag
      841 accgtccggg tgacagggcg ctgaacggtg taatgaacct cgcgcggaac cgtgcgatag
      901 gtaggccgtt ggactgtgta cggttcgtcg cgacactgct gttcgacaac attccggacc
      961 gttgtaatcg tcttgggctg caagaccgtc tcgtagacgg tccgcaccac ggggacctgc
     1021 tgagagtcgt agacggtctc ctgcacattc tgaagcaccg tctgacgctg gagttgatat
     1081 tgggtgtgcc cactgcactc ctggggcacg ggacagatac ggtacgatgc accacccgcg
     1141 tagctcgctc cctgcgccgg gtgcgcggtc acgctcagcc cgaatcccat cagggcgaga
     1201 agcgcgaccg acggcagttt tctcaacatc tgacgctcca caaaacactc cgaccctatc
     1261 tcggtgctca tcggatcccg aacccaaagg ctccgccttc tgctcgggcc cgccaactca
     1321 ctgttaaagt tcgtctaatt cggagcgttt gcttcaagaa ctttctccaa atttcccaat
     1381 ggtccgaatt tcccaaaccg taatgtttac ctagatgtta cgtcgctcat ttacacgtcc
     1441 tgaattatca gaaaatccgg ccgagtgacc ggattcgtca gggcatgatt gttcgacaat
     1501 ctttcgccga tcggagttct tgaagggaaa gacgatagag tcgagtcttg gcgatcaggg
     1561 tcttatctct cgaccggtct ttgaagtggc ggcggtcggt gtgaacgcct gggttcgcag
     1621 acggccacgg tgtgggctcg tccccatgga agtcttgccg aggtcgagga acgattcgaa
     1681 tgtcgcgtgc gttaaccggc ggagtcgatt ggacagtgtc ctgcggaaca ggagcaaacg
     1741 ggcacgtggc tcggtggagt tggtggcgga atggggataa gccggcgacc caaagcccat
     1801 gtcgattctc cggattcgcg gacgtagtcc cagagaaaac tattcgtcgg gacgcttgcc
     1861 atggaaatgg cctggcagcc ggagaatcct tggtggagcg tggcgaatag caaatcatga
     1921 gcaacgggat cggcaggtaa gaggggaacg ctgcccagaa tccggaccgc tggcgaccgg
     1981 ctctccgcca agaccttgcc tgagatctcg aactttaggc ccgagggggc gatacacagc
     2041 ggaacgacgc cgcaaatcga tccggcggca tcacgaaccg tggcgaggta aaatcgggac
     2101 ggatcgttgg tgctgcggag atgatcgacg aattccgggc acttccccag aaggtcggag
     2161 tctagacttc ccgcgatcag cctcgcccac cctaatcgaa cctccggcga atcccagtcg
     2221 tcctccgaaa cgcgaatcga gtacccacgt cgcataaaca tatccagaaa taccaataat
     2281 aaacattcgt gcataacatc acgtaagcga tccaaatact gcgcgccgca actacctgct
     2341 agtaatcctg cgatcagcac ggaaaagtca cagggacggc gtgagaattg acccgcgttg
     2401 ctttttcgcc tcgcaagttt ccttcgaaag cttggctcgt cgcgtcgaac tcgtaagaga
     2461 aggcttcgat tgagaacgca aaacccaccg accgaacgcg cgttcggcca agaatcctgc
     2521 agccgcgcgt ggatcagcgt cttacccgcc cgcccgcatg cgattgcaaa cagtatctca
     2581 gcctgctcgc ttcccgttcg agggtctttc cgtttggcgc gacgtcggat aatctgaggc
     2641 gctttggcga ggcaacggcg agcaaactta tccacgagaa cccaagatga gcggcagcgt
     2701 gctggaccga tttcggctcg acggcaaggt ggctctggtc actgggggcg cgcggggcct
     2761 gggaagggtg atcgccgatg cattagcttc ggcgggggct gacgtggcga tcaccgcccg
     2821 tcaggcagaa agtgctcagg gagcagccga gctcattgcg gctatgtcga cgcgcaaggc
     2881 gatcggattg gccgccgacg taacccagcg atcatccgtc gaggcgctgg tcgcccacgt
     2941 gctggaaaca ctcggccggc tcgatatcct ggtcaacaac gccggggtca atatccgcgg
     3001 cccgatcgaa gaactcagcg aggacgactg ggacacagtc atcgacacca acttgaaagg
     3061 tccgtggctg tgctgccgcg ccgtcgcccc agtgatgaag cgacagaaat cgggacgggt
     3121 tatcaatgtc tcgagcatgc tcggcgagat ttcgatgccg ggccggtcgc catatgcctc
     3181 gagcaaaggg gggctgacgc ttttgaccaa gaccttggca ctcgaatggg ccaaggacgg
     3241 gatcaacgtc aacgccctat gccccggtcc gttcgcgacc gagatcaaca cgccgctctt
     3301 gaacgatccg gctgcccgcg cgcagatgga agccaatgtg cccctggccc gctggggtga
     3361 ccccgtcgag ctggggccgg ccgcggtttt tctcgcgtcc gaggcctcga gcttcatgac
     3421 cggcgcgacc ctgttcatcg atggcggcta taccgcccga taaagggaga cgaacctcag
     3481 aagacgcgtg cgtgtcaagc cgacgaagcg cttttgtcca tcgacaaatc agccatggag
     3541 tcgattttca tgagtgacag cgcgcaattg gccatcccac gcggcttccg cgcgagtgga
     3601 gtcaaagcgg gattgaaggc atcgggctcg ctcgatctgg cggtgctggc ggcggatcaa
     3661 ccgtgtgctg cggccggtac gttcacgacc aaccgagtct gtgcggcccc ggtgaagtgg
     3721 tgccgagagc tgctccccgc cgacaacatt cgcgcgatcg cgatcaacgc gggcaatgcc
     3781 aatgccgcca ccggcgagca gggattagcc aacgctcggc gcacagccga gcgagtcgca
     3841 aggctcctgg gttgtcagcc tgaggaagtc ctggtcgcat cgactggcat cattggccac
     3901 caactcgcga tggataaga
//
LOCUS       DATAQL010000435         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1724273, whole genome shotgun sequence.
ACCESSION   DATAQL010000435 DATAQL010000000
VERSION     DATAQL010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724273"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VHS97_07540"
     CDS             complement(<1..836)
                     /locus_tag="VHS97_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448092.1"
                     /translation="MRPALKNLPQVLARSQLHRRLWAWTPFYTKYVSAHGLPVVGSAK
                     VSDHALREAAFLIDQLLDHRPEIRDAMNKSKMRVAVMAYSERTTELPEHRNLKPKEYW
                     DWRARGLGASRQTPVVSCGEENLLGYRGDPYSTENILIHEFGHGIDSVGLRAVDPTFR
                     TRLRQAFKDAQDKGLWKGTYANSNAAEYWAEGVQSWFNTNRQNDAQHNHVDTREELKA
                     YDPALAKLCDEVFGDGPWRYVRPDRRKEKAHLAGYDPAGAPAFEWEPALREARTKHYE
                     KV"
     gene            complement(975..2615)
                     /locus_tag="VHS97_07545"
     CDS             complement(975..2615)
                     /locus_tag="VHS97_07545"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX3448093.1"
                     /translation="MNPNLQRLVVLGLVAGFGAVAARGDTYPRQPGVDVLHYVFRVTL
                     NDQTDSIVGETTVEVRFLENNVTAMSLDLASMAEDKGMVVTAVTSASAPAPYEHKEDR
                     LKITIDPAPKAGERRTFAILYCGVPRAGLRIGKNRHDDRTFFSENWPDKARHWLPTLD
                     HPSDKATSEFVVTAPARYQVVANGLLQEERDLGDGRRLTHWKESVPIATWLNAIGVAQ
                     FAAHHAGTVKGVPLETWVYHQDRDPIVSALESPGRRVIEFYAEHVGPYPYEKLAGVQA
                     AGVGGGMEHASAIFYGERSVYGRDVTNLVAHEIAHQWFGDSVTERDWDDVWLSEGFAT
                     YFTLLFTEHDRGRDAFVAGLKRSREIVFTTEGRNPGLAVIHNNLADTRKVVNRLVYDK
                     GGWTLHMLRNLIGTEAFWAGIQDYYRRYRDGNASTDEFRQVMEENSHRDLSWFFRQWL
                     KQPGSPVVEGSWQYRPEDKRIAIELAQVQSGDPYRLPLEIGISIVGSNEARIEKLELT
                     ERKQQFDVPAEKTPSAVILDPNCWILMKADWKPKSKTP"
     gene            complement(2612..>4014)
                     /locus_tag="VHS97_07550"
     CDS             complement(2612..>4014)
                     /locus_tag="VHS97_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX3448094.1"
                     /translation="GAPDLPYQIDFPPEEFQARWNTVFDRIGDHAIAIVQGAPKANGF
                     LLPRQSNEFYHLCGIETPLAYLILDGRDRRASLLLPPRDARLESAEGKIISADDAELV
                     KRLTGAKTVASTTSMTEDWMRQLIGSSITAICTPFAPAEGNAQSRGELRSANNAILLD
                     PLDGRPAREAHFVELLKSRFPKVEIRDLTPILDELRSVKSPREIALIRRASQLAGWGL
                     IAAIKNTRPGAFEYQLDAVARYVFQVNGARLEGYRSITAGGTDNIWNMHYYRNADQLK
                     SGDLVLMDFAPEYHYYTSDIARMWPVSGKYSAPQRELLQFVLDYRNCILKRIKPGVTT
                     RAIQADAQTAMEEVFAHTKFSKPIYEKAARRLVQSGGGVFSHPVGMAVHDDGGYNRGV
                     LKPGQVFSIDPQLRVPEERLYYRYEDVIVITSAGYENFTDFLPTELDEIEKLVGQGGV
                     LEAFPPLGDHQQARRP"
BASE COUNT          759 a         1210 c         1191 g          854 t
ORIGIN      
        1 ttcacttttt cgtagtgctt ggtgcgagcc tcacgtagtg cgggctccca ctcgaatgcc
       61 ggcgcaccgg ctggatcata tccggccagg tgagcctttt ccttgcggcg atcggggcgg
      121 acatagcgcc aagggccgtc accgaaaacc tcgtcgcaca gctttgccag cgcggggtcg
      181 taggccttga gctcttcgcg agtgtcgacg tggttgtgct gagcgtcgtt ctggcggttc
      241 gtattaaacc acgactgaac tccttccgcc cagtactcag cggcattgct gttggcgtac
      301 gtccctttcc agaggccttt gtcttgggca tccttgaagg cctgtcgaag gcgtgtgcga
      361 aaggtcgggt cgacggcgcg cagacctacg gaatcgatcc cgtggccgaa ctcgtggatc
      421 aagatgtttt cggtcgagta ggggtcgccg cgatagccga gcagattctc ctcaccgcaa
      481 ctcaccacag gcgtttgacg gctggcgccc aaaccgcggg cgcgccagtc ccagtactct
      541 ttcggcttca gattccggtg ctcggggagt tctgtcgtcc gttcggaata agccatgaca
      601 gcgacgcgca tcttgctctt attcatcgcg tcgcgaattt ccggccggtg atccagcaac
      661 tggtcgatca agaacgcggc ctcgcgcagc gcatggtcgg agacctttgc cgagcccacc
      721 accggcaagc cgtgcgcgct cacatacttg gtgtaaaaag gggtccaggc ccagagacgc
      781 cggtggagct gtgaccgcgc caacacctgt ggcaggttct tgagcgcggg tcgcaatgat
      841 tgtcgtcaac tgcaatagaa gcgctgccac accggcggca atcgaccaac gggaccccat
      901 cagccacctc gcttttcgta gagcaagatt attgctttat caccataaga ataagtcatt
      961 gagcgatacg gttttcaggg cgtcttcgat ttcggcttcc agtccgcctt catcaagatc
     1021 cagcaattgg gatcgagaat gaccgcggac ggggttttct ccgccgggac gtcgaattgc
     1081 tgctttcgct ctgtcagctc cagtttttcg atccgggcct cgttagatcc cacaatcgat
     1141 atgccgatct ccagaggcaa gcgataaggg tcgccgctct gaacctgggc aagctcgatt
     1201 gcgattcgct tgtcctcggg acgatactgc catgagccct cgacaaccgg cgaccccggc
     1261 tgcttgagcc actggcggaa aaaccacgag aggtcgcggt gggagttttc ctccatcacc
     1321 tggcgaaact catcggtgga ggcgttgcca tcgcgatacc gccgataata atcttgaatt
     1381 cctgcccaga aggcctcggt gccgatcaga tttctgagca tatgcagagt ccagcctccc
     1441 ttgtcgtaga ccaggcggtt cacgaccttc cgcgtgtcgg ccagattgtt gtggatcacg
     1501 gcaaggccgg ggttgcgccc ttcggtcgtg aagacgatct cgcgacttcg cttcaaccct
     1561 gccacaaacg cgtcgcggcc gcgatcatgc tccgtgaaga gcagcgtgaa ataggtcgcg
     1621 aatccctcgc tgagccacac gtcatcccag tcgcgttcgg tgacggagtc gccgaaccac
     1681 tggtgggcga tttcgtgggc gaccaggttt gtgacgtctc tgccgtaaac agaacgttcg
     1741 ccgtaaaaga tggcgctggc gtgctccatg ccgccgccca cacccgcggc ttgcacgcca
     1801 gctagctttt catagggata gggacccaca tgttccgcgt agaattcgat gacgcgccgc
     1861 cccgggcttt cgagtgcaga gacgatcggg tcgcggtcct ggtgataaac ccaggtctcc
     1921 aggggcactc ctttgaccgt gcccgcgtga tgtgcggcaa actgagccac gccgatcgcg
     1981 tttaaccacg tcgcgatcgg cacggattct ttccagtgcg tcagccgccg gccgtccccc
     2041 aggtcacgct cttcttggag caagccattg gcaacgacct gatagcgcgc gggagccgtg
     2101 accacgaatt cgctggtcgc cttgtcgctg ggatggtcca gggtcggcag ccaatgccga
     2161 gccttgtccg gccagttctc actgaaaaac gtgcggtcgt cgtgacggtt cttgccgatg
     2221 cgcaggccag ctcgcggtac gccgcaatag aggatcgcga acgtgcgccg ctcgcccgct
     2281 ttcggcgccg gatcgattgt gatcttgagc cgatcttcct tgtgctcgta gggcgccgga
     2341 gcgctggccg aagtcaccgc ggtcaccacc atccccttat cctccgccat cgatgcgagg
     2401 tcgagcgaca ttgctgtgac attgttttcc aaaaagcgca cctcgaccgt tgtctcccca
     2461 acgatagaat cggtctggtc gttcaaggtg acccgaaaaa cataatggag gacgtcgact
     2521 cccggctggc gtgggtaggt atcgccgcgc gccgcaacgg cgccgaatcc ggcaaccaat
     2581 cccagaacca caagtctttg aaggtttggg ttcacggccg gcgcgcctgt tggtgatcac
     2641 cgagcggagg aaatgcctcg agcactcctc cctggccgac cagcttttcg atctcgtcga
     2701 gctcggtcgg caggaagtcg gtgaagtttt cataacctgc cgaggtgatc acgatgacgt
     2761 cctcgtaccg atagtacagt ctttcctccg gcacacggag ctgcggatcg atcgaaaaga
     2821 cctggccggg tttcaaaaca ccgcggttgt agccgccgtc atcatgaacc gccatgccta
     2881 ctggatggga aaagacccct ccgccggact gcaccagtcg gcgcgcggcc ttttcgtaaa
     2941 ttggcttgga aaacttcgta tgtgcgaata cctcctccat ggcggtctgc gcatcggcct
     3001 ggatcgccct ggtcgtcact ccgggcttga tgcgcttcaa tatgcagttg cggtagtcga
     3061 ggacgaactg caatagttca cgctgaggcg cgctatactt gccgcttacc ggccacatgc
     3121 gcgcgatgtc gctggtgtag taatgatatt cgggtgcgaa gtccatcaga accagatcgc
     3181 cgctcttcaa ttgatcggcg tttctgtagt agtgcatatt ccaaatattg tcagtaccac
     3241 cggccgtaat cgagcgatag ccttccagac gcgcaccatt gacctgaaag acgtaccgag
     3301 cgacggcgtc gagctgatac tcgaaggcgc ccggtcgcgt gttcttgatc gcggcaatca
     3361 aaccccagcc ggcgagctgt gaggcccgcc tgatcaaggc gatttcgcgc gggctcttca
     3421 cactgcgcag ctcgtcgagg atgggtgtca agtcgcgtat ctcgaccttt ggaaagcggg
     3481 acttcaagag ctcgacgaaa tgagcctcgc gcgcggggcg gccgtccaat ggatccagca
     3541 agatggcgtt attggccgat cggagctcgc ctctgctctg cgcattgcct tcggcgggtg
     3601 cgaatggtgt acagatcgcg gtgatcgagc tgccgatcaa ctgacgcatc caatcttcgg
     3661 tcatcgatgt agtgctggct acggttttgg cacctgtcag gcgcttaacg agctccgcgt
     3721 cgtctgccga gatgattttt ccctcggccg attcgagccg tgcgtcgcgc ggaggaagca
     3781 atagactcgc ccggcgatcc cggccatcga ggatcagata ggccagtgga gtctcgatcc
     3841 cgcaaaggtg gtaaaactcg ttggactggc gcggtagcag aaaaccgttg gccttgggcg
     3901 ctccctgcac gatggcgatg gcgtggtcgc cgatcctgtc gaacactgta ttccaccgag
     3961 cctggaactc ctccggtgga aaatcgatct gataggggag gtcgggcgcc cccg
//
LOCUS       DATAQL010000436         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4471354, whole genome shotgun sequence.
ACCESSION   DATAQL010000436 DATAQL010000000
VERSION     DATAQL010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4471354"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VHS97_07555"
     CDS             <1..595
                     /locus_tag="VHS97_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3448095.1"
                     /translation="PHTLGLKAWHFIKANRSVTHPDATVMLPRASKSVDWEVELAAVI
                     GRPAKDVGESQALDYVTGYLIANDLSARDLGFREQLPPANPFRTDWLAHKSFDGSCPL
                     GPWIVPASEIGDPQNLGLKLWVNDVLKQDSNTGMMIFTLAEQIAQLSSRITLYPGDVI
                     LTGTPAGVGAGRGEFLKPGDVVKLWIEKIGTLTNTMT"
     gene            592..825
                     /locus_tag="VHS97_07560"
     CDS             592..825
                     /locus_tag="VHS97_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448096.1"
                     /translation="MTPGRAGPANGSGGRRVVDGAVPPRFGGAVTRAAPCRDIPFGGK
                     LLQDCNHVAADQAAARRRYGATPVRRTFAAYIL"
     gene            complement(862..>1547)
                     /locus_tag="VHS97_07565"
     CDS             complement(862..>1547)
                     /locus_tag="VHS97_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3448097.1"
                     /translation="SVTIIAAPLYQIALVVARFRGNVDPYFASEACCHEWPKMTRWRR
                     NPGISDRVETLMAMTEEMTTPASTDKPRPALKLKFLSHGTLESRDLVFSRKFYEEFLG
                     LDVVQTSPISLLIRLGGNHTYAVVYSKQPREMNLLNHNGLDVRTKEEVDAAHRVVVES
                     AQKWKLTKISTPHLQHGTYSFYFWDADGNSWEILTNPEGGYSWLFEKGDLEGEGHLDR
                     NFKRPGVDI"
BASE COUNT          283 a          473 c          494 g          297 t
ORIGIN      
        1 tccgcatacg ctcgggctca aggcgtggca tttcatcaag gccaatcggt cggtgacgca
       61 tcccgacgcg acggtgatgc tgccgcgcgc gtcgaaatcg gtcgactggg aggtcgagct
      121 ggccgcggtg atcggccgcc cggccaaaga cgtcggcgag tcacaagcgc tcgactacgt
      181 caccggctat ctaatcgcca atgacctgtc ggcacgcgac ctcggctttc gcgaacagct
      241 gccgccagcc aacccgtttc gcaccgactg gctcgcccac aaatcattcg atgggtcctg
      301 cccactcggc ccgtggatcg tgccggcgag cgagatcggc gacccccaga acctggggct
      361 gaagctgtgg gtcaacgacg tgctaaagca ggattcgaac accggcatga tgatcttcac
      421 gctggccgag cagatcgcgc aactgtcgag ccgcatcacc ctctatcctg gcgatgtgat
      481 tctgaccggg acccccgcgg gggtcggggc cgggcgcggc gaattcctca agcccggtga
      541 tgtggtcaag ctgtggatcg aaaagatcgg cacgctgacg aacacgatga cgtgactccc
      601 gggcgggccg ggccggcgaa cggctcgggc ggccgccgcg ttgtcgacgg cgcggtgcca
      661 ccgcgattcg gcggtgccgt aacccgagcc gcgccttgcc gcgatattcc cttcggcggc
      721 aagctgctcc aggactgcaa ccacgttgct gcggaccagg ccgcagcgcg gcgacgatat
      781 ggcgcgacac cggttcggcg caccttcgct gcctacatac tgtgagacgg gtcgctcagt
      841 actccggcgc acaggtgtga cttagatgtc gacgcccggt cgcttgaagt tccgatcgag
      901 atggccctcg ccctcaagat cgcccttctc gaagagccag ctatagccgc cctcgggatt
      961 ggtgaggatc tcccaggaat tgccgtcggc gtcccagaag tagaagctgt aggtcccgtg
     1021 ctggagatgg ggcgtcgaga ttttggtgag tttccatttt tgcgcggact cgacgacgac
     1081 gcgatgcgcc gcgtccactt cctccttggt gcggacatcg agaccgttgt ggttgaggag
     1141 gttcatctcg cggggttgct tgctgtagac gaccgcgtag gtgtgattgc caccaagccg
     1201 gatgagcagc gaaatcgggc tcgtctgcac cacgtcaagg ccgaggaatt cctcataaaa
     1261 cttgcgcgag aagacgaggt cgcggctttc gagtgtgccg tgcgacagga acttgagctt
     1321 gagagctggg cgcggcttat cggtgctagc cggcgtggtc atctcttcgg tcattgccat
     1381 gagcgtctcc actcggtctg agattcccgg atttcgacgc caacgtgtca ttttgggcca
     1441 ttcgtggcaa catgcttcgc tcgcgaaata cgggtcgaca ttgccgcgaa agcgtgcaac
     1501 gaccaatgca atctggtaca atggcgctgc aataatcgtc actgaga
//
LOCUS       DATAQL010000437         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1876355, whole genome shotgun sequence.
ACCESSION   DATAQL010000437 DATAQL010000000
VERSION     DATAQL010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876355"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VHS97_07570"
     CDS             complement(<1..1210)
                     /locus_tag="VHS97_07570"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX3448098.1"
                     /translation="MKINDETCYRALVARDTRFDGLFYVGVTTTGIYCRPICTARTPL
                     PGRCRFFSNSALAEQEGFRPCLRCRPELAPGHAPVDAVPRMARAAARRIEAGALNDGR
                     GLERLAEDLGTSTRQLRRAVRREFGVSPVELAQTQRLLLAKQLLTESNLPIIRVAFAS
                     GFDSVRRFNALFRSHYRLTPTRMRRATTRNAGDCVRLTLAYRPPLAWSELMRFLKGRA
                     TQGVEGVEGNAYTRTAVVGDHQGWLKVRPNAGRNTLSIEVATSLVPALPEVLARVKSL
                     FDLNARPDVIASHLADDARLGCVAATCPGLRVPGAFDGFELAVRAILGQRVSVTAATS
                     LAGRLVGRLGEPIETPVPALHRLFPPPARLASVEESELRSLGISGARAATISAIARAV
                     LGRQIDLEPSP"
     gene            1544..>2002
                     /locus_tag="VHS97_07575"
     CDS             1544..>2002
                     /locus_tag="VHS97_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX3448099.1"
                     /translation="MTGCSSDMILAQLLDEQLNEVDHSSIAEHVQTCVRCQERLKELT
                     SQASGVLDWEPFDQLSSDPFITTDHLLSGAPESALLMDHFDPNETGGPSAQVLSESEL
                     PEVDAYEILAELGHGGMGVVYKAYQQRLSRLVALKMIRAGNLAKPEDLARF"
BASE COUNT          392 a          616 c          635 g          359 t
ORIGIN      
        1 cggggctcgg ctcgaggtcg atctggcgcc cgagcacggc tcgggcgatg gcgctgatgg
       61 ttgcggcgcg agctccgctg atcccgagcg acctcaactc cgattcctcg acagacgcta
      121 gtcgtgctgg cggagggaac agccggtgca aggccgggac cggcgtctcg atcggctcgc
      181 ccagccggcc caccaggcgg ccggccagac tcgtcgccgc cgttaccgac acgcgctggc
      241 cgaggatggc gcgcaccgcc aactcgaaac cgtcgaacgc gcccgggact cgcaatccag
      301 ggcaagtcgc ggcaacacac cccaatcggg catcgtctgc aagatgcgag gcgatcacgt
      361 ccggccgcgc gttcaaatcg aaaaggctct tcacccgggc cagaacctcg ggcagcgcgg
      421 gcacgagcga cgtcgccacc tcgatggaca gtgtgtttcg acctgcgttc ggcctgacct
      481 tgagccaacc ttggtggtct cccacaacgg ccgtccgggt gtaggcgtta ccttcgaccc
      541 cttcgactcc ctgtgtggcc cttcctttga ggaagcgcat taactcggac caggccaggg
      601 ggggtcgata tgccagcgtg agccgcacgc agtcgcccgc gttcctcgtt gtcgcgcggc
      661 gcatccgggt cggggtaagg cgatagtgag agcgaaagag cgcattgaac cggcggacac
      721 tgtcaaagcc gctcgcgaac gcgacccgga tgatcggcag attcgactcc gtgagcaatt
      781 gtttggcgag gagaaggcgc tgagtctgcg ccagctcgac gggcgaaacg ccgaactcgc
      841 ggcgtaccgc gcggcggagc tgccgcgtgc tggtgcctag gtcctcggcc aaacgctcca
      901 gtccccggcc atcgttgagc gcaccggctt cgatgcggcg agcagccgcc cgcgccatgc
      961 gcggaaccgc atcaaccgga gcgtgaccag gagccagctc cggtcgacag cgcaggcacg
     1021 gccggaaccc ctcttgctcg gccagggccg agttcgagaa aaaccggcac cgaccgggca
     1081 aaggggtccg cgcagtgcag atgggccggc aatagatccc cgtagtcgta acgccgacat
     1141 agaacaagcc gtcgaaacgg gtatcacgcg ccaccagggc ccgatagcaa gtctcatcat
     1201 tgattttcat gacctgattc tagcggtgaa tgacgccctg ttctggccat tttcggactt
     1261 caagacaaat ttgtccggtc gcgacgcgac gtctggcaac ataattcggg aggaaattac
     1321 tccctgccgt gtaaggtcct gtcggcaaga gccgtattgt ttcgatgaga gagtagggtg
     1381 atcgcccggg agtcggccgg acctcttatt tgcgcgaggg agctcgcatg cgcccaggcg
     1441 cagccgtggt gaacgactca tacggacgac gacccatagc cccatttgca tccggccttt
     1501 gcggccggaa caacggttga cacggacgca ccggggaaaa gcaatgactg gctgctcaag
     1561 cgacatgatt cttgcgcaac tgctcgatga gcagttgaac gaggtggatc attcgtcgat
     1621 tgctgagcac gtgcagacct gcgtccgctg ccaggagcgg ctgaaggagc tcaccagcca
     1681 ggcatctgga gttctcgatt gggaaccgtt cgatcaatta tccagcgatc cttttataac
     1741 cacggatcac ctgctctctg gtgctccgga atccgcactt ttgatggacc attttgaccc
     1801 gaatgagacc ggtggcccat cggcacaggt cttgtctgaa tcggagctgc cggaggtcga
     1861 tgcctacgag attctggctg agctcggtca tggtgggatg ggagtcgtct acaaagcgta
     1921 ccagcagcgt ttgagccgac ttgtggcact taaaatgatt cgcgctggga atttggcgaa
     1981 acccgaggat cttgcacggt tt
//
LOCUS       DATAQL010000438         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2182077, whole genome shotgun sequence.
ACCESSION   DATAQL010000438 DATAQL010000000
VERSION     DATAQL010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182077"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1901
                     /locus_tag="VHS97_07580"
     CDS             372..1901
                     /locus_tag="VHS97_07580"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3448100.1"
                     /translation="MPEDLKAGPTRLSAPQFLEALEGSGLLSAAMLRQVQDRFVNRTD
                     PDDSLALARELVDEGTLTEFQARRLLKGKRGFAFGRYVLLDQIGQGARGRVFKARHRL
                     MDRVVALKVLVWDDTLSESSVSRFFREMKIVALLDHPNVVRAIDADVHEGSPYIVMEY
                     LEGDDLEHVLARRGPLPPDEVIDAMARVSRGLSHAHDKGVIHRDVKPTNVFLLKTGEA
                     KLLDLGFGELVGMAGQTGNVFDTDEDIVVGTTDFMSPEQVRNKGIDARTDLFSLGCTM
                     YRLLTAAYAFPGVTREDRLIKRIQEPHVPITDVRPGLSYRLVAIVDRLLAIHPDDRFA
                     SAAEAADALEALIPPARRSELATSVRPGTMRSGADVASLHSEPDVPLDWSRIESALRS
                     TGHATRESTRLVNRNEPKPPSAKGLSAHRKMLEAEGVESGREVHEQYRSAVIQMKRES
                     AELRSADPTNDALSADATWLERIGEWFGDFLAEPSAAQIIIAILGLLVVLALALAYAL
                     A"
BASE COUNT          369 a          624 c          615 g          370 t
ORIGIN      
        1 aaggtgtagg acatcggcga gtgcaattct tgccccgcac gtgggtcgct cgagccggta
       61 accgtcggag cgacgcgggg gaggaaacga aactgccgag cctttaagac agtcgagtcg
      121 cctggcgact tggcgatcaa gcaggccata acccgagtag acaatgcatg tactttgatc
      181 ctagtttgat gggggttata ccgaccctgc tctcacgacc gttcgcgcat cctcgtctgg
      241 ctttctttgc ctcgtcggct tattctctgt agtgtcggaa atccaagcaa tgagatctcc
      301 acgccacaaa aagtgtgcga gcctgcaatc aaactcggat gaatcgtgca atgatccgga
      361 gcggtcgcgc catgcctgaa gacctgaagg ccgggccaac gcgcctctcc gctcctcagt
      421 tcctcgaggc cctcgaaggc tcgggactcc tgtccgctgc catgttgcgg caagtgcagg
      481 accgattcgt gaacaggacg gacccggatg attcattggc ccttgcccgt gagctcgtcg
      541 acgaagggac actcactgag ttccaggccc ggcggctttt gaaagggaag aggggctttg
      601 ccttcgggcg ctatgttttg ctcgaccaga tcggccaggg ggcgcggggc cgggtgttca
      661 aggcacggca ccgcctgatg gatcgcgtgg tcgccctcaa agtgctcgtg tgggatgata
      721 cgctcagcga gtcctctgtc tcgcggttct tccgtgagat gaagatcgtc gccctcctcg
      781 accaccccaa cgtggtccgg gccatcgatg ccgacgtgca cgagggctct ccgtacatcg
      841 tcatggagta tctggaaggg gacgacctgg aacacgtcct tgcgcgccgc gggcccctac
      901 cgccggatga ggtcatcgac gccatggcgc gggtgtcgcg cggtctctct cacgctcatg
      961 acaagggggt gatccaccgc gatgtcaaac ccacgaacgt gttcctgctc aaaaccgggg
     1021 aagcgaagct gctcgacctc ggcttcgggg agttggtggg gatggccggg cagactggca
     1081 acgttttcga tactgacgaa gacattgtag tcgggacgac cgacttcatg tcgcccgagc
     1141 aggtccggaa taaggggatc gatgctcgga cagacctttt cagcctcggc tgcacgatgt
     1201 accgcctgct cacggcggcc tatgccttcc ccggcgtgac cagggaggat cggctcatca
     1261 agcggatcca ggagcctcat gtcccgatca cagacgtccg gccgggcctg tcgtaccgcc
     1321 tggtcgcgat cgtcgaccgt ctgctggcga tccacccgga cgatcgcttc gcctcggccg
     1381 ccgaagccgc ggatgccctt gaggcgctga tcccgcccgc ccgccgatct gagctcgcga
     1441 caagtgtgag accaggcaca atgcgttctg gcgccgacgt ggcatccctt cattcggagc
     1501 cggatgtgcc actcgactgg tcgcggatcg aatcggcact ccggtcgact ggtcacgcca
     1561 cccgggaatc cacccggctg gtgaacagga acgagccgaa accgccctcg gccaagggtc
     1621 tctccgccca tcgcaagatg ctcgaagccg agggtgtcga atccgggcgg gaagtgcacg
     1681 aacaatatcg cagcgcggtg atccagatga agcgggaatc ggccgagctc cgctcggcgg
     1741 accccacgaa tgacgcactc tcagccgacg cgacctggct cgagcgaatc ggggagtggt
     1801 ttggcgactt cctagcggag ccgagcgcag cgcagataat tatcgccatc ctgggcctgc
     1861 tcgtcgtcct ggcgctggcc ctggcttacg cgctcgcata gcctccaccg cgacgaatcc
     1921 tcatcgaaag acgctcagta cgagctggcg tcaagaacct cgcctcctgc cactgtgc
//
LOCUS       DATAQL010000439         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5082824, whole genome shotgun sequence.
ACCESSION   DATAQL010000439 DATAQL010000000
VERSION     DATAQL010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5082824"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VHS97_07585"
     CDS             complement(<1..225)
                     /locus_tag="VHS97_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448101.1"
                     /translation="MRLGPGPVFVYEWLTTSRRWQLYGLRAAFVGAILIGMMFVWHQS
                     RTFANPTQAVSIQTLARYGEELYETIVSIEL"
     gene            638..2602
                     /locus_tag="VHS97_07590"
     CDS             638..2602
                     /locus_tag="VHS97_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX3448102.1"
                     /translation="MNGILKGGYPSVVGDMVRELETRFPWLSPWLGDRLDTEVPAFVV
                     SLTMHGLLLIGLAFAGYRVQRQAQREFTSAVVDNLVTPDSTFQDLDQSANPPPPEAAA
                     GSFAPTLALSITSAPSTAGGVPVSSSDDSNHGLAPELVRLDVRRATEVVVPTATMLGQ
                     TVSIRGNGAEMVGGVEGAVDRVAVEILRRLEQGRTLVVWAFDASGSLQAERQRLGKHI
                     ETIYTHINQLDANRLSDDNGLLTMVVSFGHDHRALLPKPTAERSEIIEAINGVPLDET
                     GIETTFATVADIVQHWGRYKDAHNLVYHTMVIVVTDEVGDDEEQLEDAVTMAQKAKVP
                     VYILGSQAVFGRTEGYMDYTDPKTKHVFRGVRVRQGPESVALEQIRLPFWYGGPQYEI
                     VEAGFGPYALSRLASATGGIYFVTRFQARRMGFDPARMREYKPDWVPRQQYEKQVANS
                     PFKQAVLAAAQITQQQLPGMPSLFFPPADSPEFKELMGVNQGVAERTAYTVDEALAPI
                     NQVVNLRDRETSRRWQAHYDLIRGRLLAMKVRCHEYNWVCARMKKDPQKFSSSKFNAW
                     RLVPDQEIQYSDKAAAAGREAQSLLRRVVEDHPATPWALLAQRELENPLGFKWMETYV
                     PPRPRNRDNDAAKRKNKNMSEAKPPQVPKL"
     gene            2703..>3678
                     /locus_tag="VHS97_07595"
     CDS             2703..>3678
                     /locus_tag="VHS97_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448103.1"
                     /translation="MELRTPTPVPDDDVYRFAETSAPVSVRPANLKSPEVNAARTLAS
                     AGDMPKTVVSDPVVMSLTGSGPTVADMGAGSSPDQAGTDRAVDAVEPTTAASAGAKDA
                     RSAATTPAHSEPGTAAGGVPRVKRLRGTRRRRRIRLRYLMQAWGVSLIVHVAILSALA
                     AATFSSKDAIKKLVSFDSALASYSGGEPEMLPIYADPDTISRDKATGNEHAATPGEIV
                     PAVLGEGGNDGEGADGGGVIVAGGAGRPSNTPRVRGVGKGRINEGSSLPGVKVDGLSG
                     SPLTLLPAAHAADLSGAGKIAGDPVFDVKEIGVALDQLAREILRHLKDH"
BASE COUNT          795 a         1076 c         1109 g          698 t
ORIGIN      
        1 cagctcgatc gatacgattg tttcgtatag ttcttcaccg taccgcgcca gtgtctggat
       61 cgagacggcc tgggtcgggt tggcaaacgt gcgcgattga tgccagacga acatcattcc
      121 aatgagaatc gccccgacaa acgcggcccg cagtccataa agctgccacc ggcgcgaggt
      181 tgtgagccac tcgtaaacaa agaccggtcc gggtcccagc cgcaacttaa atcccccgat
      241 aaaagagcat tccgcgctct gtgcttcctt tgcttatatc gctgaattct tccagacatt
      301 gcggacgatt gtcaaacatc ccgctgcact caagtgaaat gcattgacca aactgctatc
      361 tggccagcac cgagggcagg gcttcctttc gcacagacca tgtcattgca ttacacagcg
      421 atgcgttagc cgacaattcg gactttccca cgagctcgac tggattgttt ttaagagccc
      481 cagcgattcg acgccagtcg attgttggat tgggttcatc gccgactgcc tgcaagtaag
      541 ctgtcacgaa ggcagtgtta agagtagtat tttagagagg tccgtggtta ccgaaggtgc
      601 gccctgatga ggatggcaca ggcttccggc agatttgttg aacggaatct taaagggggg
      661 gtatccatcc gtggtgggtg atatggtgcg ggagctcgag acgcggtttc cgtggctgtc
      721 gccatggctg ggagaccggc tggacacgga ggtacctgcg ttcgtcgtga gcctgaccat
      781 gcacggcctg ttattaatcg ggctagcttt tgccggctac cgggtgcagc gccaggccca
      841 gcgcgagttc acgagcgctg tcgtggataa cctggtgacg ccggattcga cgtttcaaga
      901 cctggaccag tcggccaatc ccccaccgcc ggaagcagca gccggatctt ttgccccaac
      961 gctcgcgctg tcaatcacct cagcaccgag tacggcaggg ggtgttccgg tctcgtcgtc
     1021 agacgattca aaccatggcc tcgcgccgga gctcgtgcgg ctcgacgtgc ggcgagcgac
     1081 cgaagtggtc gtacccacgg cgacaatgct cggacagaca gtgtcgatcc gaggcaatgg
     1141 tgccgagatg gtcggaggcg tcgagggagc agtcgaccgg gtcgccgtcg aaatcctgcg
     1201 ccggctggag caggggcgca cattagttgt atgggcattc gatgcctctg gaagcctcca
     1261 agccgagcga cagagactgg gcaagcacat cgagacgatt tatacccata tcaatcaact
     1321 tgatgctaac cggctgtctg acgataacgg actgctcacg atggttgtct ctttcgggca
     1381 cgatcaccga gcgctccttc ccaagccaac ggccgagcgg tccgagatca tcgaggcgat
     1441 caacggcgtt ccccttgatg agaccggcat cgagacgacg ttcgcgacag ttgccgatat
     1501 tgttcaacat tggggccgct acaaggacgc gcacaacctg gtttatcaca ccatggtcat
     1561 tgtggtcacc gacgaggtcg gcgacgacga agagcagctc gaagatgccg tgacgatggc
     1621 acagaaagcc aaagtacccg tgtacatctt gggctcgcag gcggtcttcg gccgcaccga
     1681 ggggtacatg gactacaccg accccaagac caagcacgtg ttccgaggcg tacgggtgcg
     1741 gcaaggaccc gagagtgtcg cgcttgaaca gatccgtctg cccttctggt acggtggtcc
     1801 ccagtatgag atcgtcgagg caggttttgg cccgtacgct ctcagcagac tggccagcgc
     1861 caccggtggg atttatttcg tcacccgctt ccaagctcgc cgcatggggt tcgatccggc
     1921 gcggatgcgt gaatacaagc ccgactgggt gccgcgccaa caatatgaga agcaggtcgc
     1981 caactcgccg tttaaacagg ccgtccttgc cgccgcccag atcacccagc agcaacttcc
     2041 gggcatgccc agtctctttt ttccaccagc cgattccccg gaattcaaag aattaatggg
     2101 agtcaaccaa ggcgtggccg agcggacggc ttacactgtc gacgaagctc tggcaccgat
     2161 caaccaagtg gtcaacttgc gcgatcgaga gacttcgcga cgctggcagg cgcactacga
     2221 cctgatccgg ggccgactgc tagccatgaa ggtgcgttgc cacgaataca actgggtctg
     2281 cgccagaatg aaaaaagatc cccaaaaatt cagcagttcc aagttcaacg catggcggct
     2341 cgttcccgac caggaaatcc agtatagtga taaggcagcg gctgccgggc gcgaggctca
     2401 atctctgctc cgccgtgtcg tagaggacca tccggctacc ccctgggccc tgctcgcgca
     2461 acgcgagttg gaaaaccctc tcggattcaa atggatggaa acttatgtgc ctccccgtcc
     2521 gcgtaatagg gataacgacg cggcgaaacg caaaaacaaa aacatgagtg aagctaagcc
     2581 gccccaagta cccaagctat aattgacccg gaatgatcaa gcggtcagtt gggccgacga
     2641 attacagccg agacgaatta tttccgctga cgtacaatct gtcagagagg ggaccgcgag
     2701 acatggaact gcgcactcct actcctgtgc ccgatgacga cgtttatcga ttcgcagaga
     2761 caagcgcgcc tgtgtccgta cgtccggcta atttgaagag ccccgaggtc aatgccgcaa
     2821 ggacgctggc gtctgcgggt gacatgccca aaacagtggt ctccgaccct gtagtcatga
     2881 gtctaacggg atcaggtccc acggtcgcgg atatgggggc ggggtcttcc cccgaccaag
     2941 ccggcaccga cagggccgtg gacgcggtgg aaccgacgac cgctgcctcg gccggtgcga
     3001 aagatgctcg gtcggcagcg accacgccgg cgcacagtga accgggtacg gctgccggcg
     3061 gcgtgccgcg cgtcaaaagg ttgcggggga cgaggcggcg tcgacgaatc cgactgcgtt
     3121 acttgatgca agcctggggc gtttcgttaa tcgtccacgt ggcgatcttg tcggcgctcg
     3181 ctgccgcgac attctcgtcg aaagatgcga tcaagaagct cgtcagcttc gactcggcac
     3241 tcgcctcgta ctccggaggc gagcccgaga tgctgccgat ttacgctgac ccagacacca
     3301 tctcccgcga taaggcaacc ggcaatgagc acgccgcgac gcctggggaa attgtcccag
     3361 ctgtgcttgg ggaaggggga aatgacggtg agggagcgga tggcggcggc gtaatcgtcg
     3421 ctggaggggc gggccggccc tcgaacaccc cgagggtacg tggcgtcggc aagggacgaa
     3481 tcaacgaagg gtcgagcctg cccggcgtca aggtcgacgg acttagcgga tcccccttga
     3541 ccttattgcc ggccgcacac gccgccgatt tgagtggtgc tggtaaaatc gccggcgacc
     3601 ctgtgttcga tgtcaaggaa atcggcgtag cgctcgacca gctcgcacgt gagattctcc
     3661 gccacttgaa agaccata
//
LOCUS       DATAQL010000440         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4013399, whole genome shotgun sequence.
ACCESSION   DATAQL010000440 DATAQL010000000
VERSION     DATAQL010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4013399"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="VHS97_07600"
     CDS             <1..1896
                     /locus_tag="VHS97_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX3448104.1"
                     /translation="KDRVDLHLPLCTASMPLAAVAQFGAPAFLPAAALLFAYTSIPTF
                     KNAREVLFEEKRLGVDVLDAVVVVGCLGTMSIFPGAVLCWCLSFGRVLVKKTQDDSKK
                     LLLNAFGKQPRYVWLYRDGTEAQIPLDRLQPLDVIAIHTGEVVPVDGIIVQGMAMIDQ
                     HALTGESTPAEKGVGDRVFAATLMVAGKVLVEVEKSGNETASAKISQILNDTAGYKLS
                     SQHKGERLADRAVIPTLAVGAVGMAAMGPGGAVAVLNSDFGTGIRMAAPLAMLSSLAL
                     CANKGILVKDGRALELMNEVDTVLFDKTGTLTRERPEVGRIIPSHGFLPERILQFAAA
                     AERRFHHPIALAILHKADELELELPATDDTQYKVGYGITVAVEGHTVRVGSKRFMDLE
                     GVEITPEVAEAVEEAHHQGHTMVMVAVDGKLGGALELHAAVRPEVRQIVQGLRDRGIK
                     HIAIISGDHEAPTRRLAESLGMDRYFAQVLPADKADHVIALQKEGRKVCFVGDGINDS
                     IALKQANVSISLRGASTIATDTAHIVFLEEGLSKLCDLRDIARDLDRNVRRSWGLILA
                     PNIACIAGVFTLGFGIMTSVITNNVAAMAALANGMLPLRKIAQIEAEKRHKLELRQAY
                     ALEREAL"
     gene            1871..2182
                     /locus_tag="VHS97_07605"
     CDS             1871..2182
                     /locus_tag="VHS97_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448105.1"
                     /translation="MRWKEKLYERPLKRPLDEQTRAAIHSEFRRRRDQIPVPAELSWH
                     VAKPELTIRSQWISFVVDFSQERMVVDAELSLAAKMFATAENRRRAVRFIESVADHLN
                     L"
     gene            2229..2744
                     /locus_tag="VHS97_07610"
     CDS             2229..2744
                     /locus_tag="VHS97_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448106.1"
                     /translation="MAHKQAQPAGNKPSLDLRFKASHQPLDPDAKRFDVFLIDSGWNS
                     SVAKLVRSHLPTMLNFEKEDSFYILTPEQSIEILKRAPQLIGHDPIILVYDLYAPPTR
                     TTRGYRGFRLNLGLIKHPEQALARLQEFLRFVAMNRTAVPLDRAIRRELYREGLDGMI
                     KILREASTELI"
     gene            2786..2986
                     /locus_tag="VHS97_07615"
     CDS             2786..2986
                     /locus_tag="VHS97_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448107.1"
                     /translation="MNAEEIPIVPILVGALGLVYFGYEVERQRHKLRQIFNVFDREES
                     EIAAMLESMVESGQLKPFVPAG"
     gene            3038..>3606
                     /locus_tag="VHS97_07620"
     CDS             3038..>3606
                     /locus_tag="VHS97_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448108.1"
                     /translation="MTANRALYPNSSSRVGVSWSDGVVRLRDDELFGTRLADLGVVFL
                     RHVFALSEVAWMEINGEQSTAAIGYDSGRLSLPDFLERLSAALRGQLPPDAAMPSAGS
                     ILRDLARPIGPLRIERFGRVLTTWEIIHHRPGRLRLRHWSIRGDLAGALRVRDATLHV
                     PGVTECTVRPVTGSVLIRFDPDSTNASHLL"
BASE COUNT          717 a         1105 c         1071 g          713 t
ORIGIN      
        1 aaggatcggg tggacctaca cctgccgctc tgcacggcct cgatgccgct ggcggccgtt
       61 gcacagttcg gtgcaccggc gtttttgccg gctgctgcgc tcctgtttgc ctatacttcg
      121 atcccgacct tcaaaaacgc gcgtgaagtt ttgttcgagg aaaagcggct gggagtcgac
      181 gttctggacg cggtcgtcgt ggtcggctgc ctggggacca tgtcgatttt cccgggagca
      241 gtgctctgct ggtgcttgag cttcggccgc gtcctggtca agaagaccca agacgactcg
      301 aaaaagctcc tgctgaacgc cttcggcaag cagccccgtt atgtgtggct ctaccgcgac
      361 ggcaccgagg cgcagatacc gctcgatcgc ctacagccct tggacgtgat cgcgatccac
      421 accggcgaag tcgttccggt cgacgggatc atcgtccaag gaatggccat gatcgatcag
      481 catgcgctca cgggcgagtc aactccggcg gagaaagggg tcggcgatcg cgtgttcgcc
      541 gcgaccttga tggtcgccgg caaagttctg gtcgaggtcg aaaaatccgg caacgaaacg
      601 gcgtcggcca agatcagcca gatcttgaac gacacggccg gatacaagct cagctcgcag
      661 cataaaggtg agcgtctggc cgacagggcc gtgatcccga ccctggccgt gggtgctgtg
      721 ggtatggcag ccatgggccc tggcggcgcg gtcgcggtgc tcaatagcga cttcggcact
      781 ggcatacgca tggccgcgcc cttggccatg ctttcctcgc tcgcgctctg cgccaacaaa
      841 gggatcctgg tcaaggatgg gcgggctctc gagctgatga acgaagtcga caccgtgctc
      901 tttgacaaaa cgggtacgct cacgcgcgag cggcccgagg tgggccgaat catccccagc
      961 catggctttc tgccggagcg aattctccag ttcgcggccg cggccgagcg caggtttcat
     1021 cacccaattg ccctggccat cctccacaag gcagacgaac tcgagctcga gctgccggcg
     1081 acggacgaca ctcaatacaa ggtcggctac ggtattaccg ttgcggtcga agggcacacg
     1141 gtccgggtag gcagcaagag attcatggat ttggagggtg tggagatcac acccgaagtg
     1201 gcggaagcag tggaagaggc ccatcatcag gggcacacca tggtgatggt ggcggtcgac
     1261 ggcaaactcg gcggagcgct cgagctccat gccgcggtca gacccgaggt gcggcaaatc
     1321 gtccaggggt tacgcgaccg cggtatcaaa catatcgcga tcatctcggg cgaccacgag
     1381 gcccccacgc gcaggctggc ggagtcattg ggcatggacc gatatttcgc ccaggtatta
     1441 cccgctgaca aagccgacca cgttatcgcc cttcaaaagg aaggccgtaa ggtatgcttt
     1501 gtgggggacg gcatcaacga ctcgatcgcg ctcaagcagg cgaatgtctc gatttcgctg
     1561 cggggagcgt cgacgatcgc caccgacaca gcccacattg tcttcctcga ggaagggctc
     1621 tccaagctct gcgacctgcg cgatatcgcc cgcgatctcg accgcaacgt ccgccggagc
     1681 tgggggttga tacttgctcc taatatcgcg tgcatcgccg gcgtcttcac gttaggattc
     1741 ggaatcatga cctcggtcat taccaacaat gtggccgcca tggccgctct ggccaacggc
     1801 atgctgccgc tgcggaagat cgcccagatt gaagccgaaa agcgacacaa actcgagttg
     1861 aggcaagcgt atgcgttgga aagagaagct ctatgagcgc ccgctcaaac gtccgcttga
     1921 cgagcagact cgggccgcca tccattccga gtttcgccgc cgccgcgatc agattccggt
     1981 gccggcggaa ctatcctggc atgtcgccaa acccgagctg acgattcgct cgcaatggat
     2041 ttcgttcgtg gtggattttt ctcaagagcg catggtcgtg gacgccgagc tttcactcgc
     2101 ggccaagatg tttgccactg ccgaaaatcg ccgtcgcgct gttcgattca tcgaaagcgt
     2161 ggccgatcat cttaaccttt gaccggcagg tacaaagccg ccaagcaatt tcgcccggga
     2221 gcacgatgat ggctcacaaa caagcccaac ccgctgggaa taagcccagc ctcgatttga
     2281 ggttcaaggc aagtcatcag ccgctcgatc ccgacgccaa acggttcgac gtctttctga
     2341 ttgactcggg ctggaatagt tcggtcgcca agctagtgcg ctcccacctg ccgacgatgc
     2401 tcaattttga aaaagaagat tcgttttaca ttctcacacc cgagcagtcg atcgagatct
     2461 tgaagcgagc tccgcaattg atcggccatg acccgatcat tcttgtttac gacctgtacg
     2521 ctcctcccac ccgcacgacg aggggttatc gcggttttcg cctgaatctc ggcctgatca
     2581 agcacccgga acaagcactt gcacgcctac aggaatttct ccgtttcgtg gccatgaatc
     2641 gtaccgcggt cccgctcgac cgcgcgatcc gccgcgagct ctatcgcgaa ggacttgacg
     2701 gtatgatcaa gattctccgc gaggcgtcga cagaattgat ctgatcgcca ttcattcgtt
     2761 gccaccctgg aacaaggaga tgcaattgaa cgccgaagaa atccccattg tgcccattct
     2821 cgtgggcgcc ctgggactcg tctattttgg ctacgaggtt gaacgacaac ggcacaagct
     2881 gcggcagatc ttcaatgttt tcgaccgcga agaaagcgaa atcgccgcga tgctagagag
     2941 catggtcgaa agcggacagc tcaaaccgtt cgtaccggcg ggctaataga taagaccggc
     3001 cgaactttgc caaaccactt ctcaaagtca ggtcaccatg accgccaacc gcgccctata
     3061 tccgaactcg tcgtcgcggg tcggggtctc ctggtccgac ggagtggtcc ggctgcgcga
     3121 cgacgaactt ttcggtacgc gactggccga cctgggcgtg gtctttttgc ggcatgtctt
     3181 cgcactcagt gaagttgcgt ggatggaaat caacggcgag cagtccacgg cggcgatcgg
     3241 ctatgattcg ggccggctgt cattgcctga ttttctcgaa cgcctttcgg cagccctccg
     3301 cggccagctt cccccggacg ccgcgatgcc gtcggccggc tcgattctgc gagaccttgc
     3361 gcggcccatc ggcccgcttc ggatcgagcg gttcggaagg gtcctgacga cgtgggagat
     3421 catccatcac cgtcccggtc gccttcgcct ccgccactgg tcgatacgcg gcgacctggc
     3481 tggggcgctg cgcgttcgcg acgccaccct gcatgtgccc ggggtgactg agtgcacagt
     3541 ccggccggtc accggcagcg tgctcatccg atttgatccg gactcgacca atgcctcgca
     3601 tcttct
//
LOCUS       DATAQL010000441         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3097332, whole genome shotgun sequence.
ACCESSION   DATAQL010000441 DATAQL010000000
VERSION     DATAQL010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097332"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VHS97_07625"
     CDS             <1..1189
                     /locus_tag="VHS97_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3448109.1"
                     /translation="SLQSSTKPDVFFGKVGRLRQPDWSFRQRGQAGLWVSDLFPYLGQ
                     VADELTMIHSMVADTSNHTPATFQENSGFRLNGFPAMGAWMSYGLGSEADDLPAFVVI
                     PDAREFPSGGAINWSNGFLPARHQGVVIRPQGEPIDDLFPASPVDPGAESAARDLVAA
                     INRRHLEEHQGEDALAARIRGYEMAARMQTSVPLVTDFEGEPAETRALYGVDRAETTD
                     FGRACLIARRLLERGVRFVQLLSGGTFGSPRRNWDGHEDMKVNHGQEALRIDRPVAGL
                     LRDLRRRGMLDDTLVLFTTEFGRTPFTQSAADVVGKGRDHNQYGFTVWLAGAGLKPGI
                     AHGSTDEIGWRAVENVVTWPDFHATVLHLLGVDHKRLTYYHNGIQRRLTNVHGEVLKP
                     ILA"
     gene            complement(1218..2357)
                     /locus_tag="VHS97_07630"
     CDS             complement(1218..2357)
                     /locus_tag="VHS97_07630"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448110.1"
                     /translation="MDLRHQPELAELLVDREAPCLSLYQPTHRSHPDNQQDPIRFRNL
                     VKTLEDSLRLKHPTHEQRQLLARFHHLADDGGFWRHTYDGLAIFGGANYFRIFKLQRA
                     VGELAIVADTFHTKPLVRIVQSADRYQVLALSRKAFRLFEGNRDVLDEVHPSQAVSQS
                     ITDALKAEPAEAHQAVVSKGSAGSVHHGSRSKKDEIDSDTERFFRAVDRAMLEHHSKP
                     SGLPLILGALTEHQTPFRAISHNSHLLPHGIEINPDALSVEALRERAWQVVEPNYLAA
                     LAKLVDEFGEAQSQWLGTDDLTSIGEAAAAGRVATLLVEANHEVPGRIDRTTGRLVFD
                     DLAHPRVDDLLDDVAEIVLKKGGNVVVVPADRMPTKSGAAAIYRF"
     gene            complement(2580..>3676)
                     /locus_tag="VHS97_07635"
     CDS             complement(2580..>3676)
                     /locus_tag="VHS97_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3448111.1"
                     /translation="LVFGPAYLDRVLYVDRPLIAPELGPPLDQSVDGVWKFGTNPRLE
                     LMDSAGYAIAVELPVDWPGPTGEIRLASPVRPGVTGRCSVRGLTWHNDLGGMGAGYAA
                     ALGGQLCSALGSESDPTSREISSRLAELGIAHDPVRVFDHCADWTLLITSGEFGDKLP
                     IGFRGCHAALELDSLDSKAARPCDLRVVASLPNRLAAQVLAAPGAGMRFFAPAMRNVV
                     DREFPLSGFAGSINVLSCNRSEWESLEDREEVAWQVSILVVTDGPAGSSIRYTTTQGE
                     PGKLTVPAFPRQRPPRDTNRAGESYAATLLSTLLEQGWNAASGVIDDGLIRSAALRSS
                     AAAALVLDRVEFGFPKGAEIDAALAAGEVA"
BASE COUNT          704 a         1118 c         1168 g          686 t
ORIGIN      
        1 atcgctgcaa tcgtcgacca aacccgatgt ctttttcggc aaggtgggcc ggctgcgcca
       61 gcctgattgg tcgttccgac agcggggcca ggcgggtctt tgggtatcgg acctattccc
      121 gtacctgggc caggtggctg acgagctcac tatgattcat tcgatggtcg ccgacacttc
      181 gaaccacacg ccggcgacct tccaggaaaa cagtggcttc cggttgaatg gattccccgc
      241 gatgggggcg tggatgagct atggcctggg aagcgaggcg gacgacctgc cggcgtttgt
      301 ggtcatcccc gacgcacgcg agttcccctc cggcggcgcg atcaactggt ccaatggctt
      361 tctacctgcc cgtcaccagg gagtcgtcat tcggcctcag ggcgagccga ttgacgattt
      421 gttccccgcg agcccggtcg acccgggggc ggagtcggct gcgcgagatt tggtcgcggc
      481 gatcaaccgc cgccacctcg aagagcacca aggcgaggac gcgctggccg cgcgcattcg
      541 cggctacgag atggccgcgc ggatgcagac gtcggttccg ctcgtcactg atttcgaagg
      601 cgagcccgcc gagacgcggg cgctttacgg cgtggatcgc gctgaaacca cggatttcgg
      661 ccgtgcctgc ctgatcgccc gccggctttt ggaacggggc gtgcggttcg ttcagctctt
      721 gtcgggcgga acgtttggta gtcccaggcg aaattgggac ggccacgagg atatgaaagt
      781 caaccatggc caggaggccc tgcgaatcga ccgtccggtc gcgggcttgc tccgagacct
      841 gcgccggagg gggatgcttg atgatacact cgtcctgttc acaacggagt ttggtcgcac
      901 gccctttacg cagtcggccg ccgatgtcgt cggtaaggga cgcgatcata accagtacgg
      961 tttcaccgtc tggctggctg gcgcaggctt gaagcccggt atcgctcacg gctcgaccga
     1021 cgagatcggc tggagggccg tcgaaaatgt cgtgacctgg cccgactttc atgcaacggt
     1081 gctgcacctg ctgggcgtcg accacaaacg tctgacatat tatcataacg gaattcagcg
     1141 ccgactgacc aacgttcatg gcgaggtctt gaagccgatc ctcgcctgag gagatcaagt
     1201 catggccgcg atgagattca aaagcgatag atcgcggcgg ctcccgattt tgttggcatc
     1261 cgatcggccg gtacgaccac gacgtttccc cccttcttca gcacgatctc ggccacgtcg
     1321 tcgagcaggt cgtcgaccct tgggtgggca aggtcgtcaa agacgagccg cccggtcgtc
     1381 cggtcgatcc ggccgggcac ctcgtgattg gcctcgacca agagtgtggc aactcgaccg
     1441 gcggcggcgg cttcgccaat tgacgtcaga tcgtcggtac ccagccactg tgactgagct
     1501 tcaccgaatt cgtcgactag tttcgcaagg gctgcgagat agttgggctc gacaacctgc
     1561 cacgcccgct ctcgcaaggc ttcgaccgag agcgcatcgg gattgatctc gattccgtgg
     1621 ggcaagagat gagaattgtg gctgattgcc cggaatggcg tttggtgttc ggtgagcgca
     1681 cctagaatca ggggcaggcc tgacggcttg gaatggtgct cgagcatcgc gcgatcgacg
     1741 gcccggaaga aacgctcggt atcgctgtcg atctcatctt ttttggagcg cgagccatga
     1801 tggacgctgc cggctgagcc cttggagacg accgcttgat gcgcttcggc cggctctgcc
     1861 ttgagcgcat cggtgattga ctgagaaacc gcctgtgagg gatggacttc atcgagaacg
     1921 tcgcgattgc cttcgaacag ccgaaatgcc ttgcggctga gtgcaagaac ctggtaacgg
     1981 tcggcggatt gcactatgcg taccaagggc ttggtatgaa aggtatctgc gacgatggcg
     2041 agctcgccga cagcccgttg caacttgaaa atccggaagt aattcgcccc cccaaagatc
     2101 gccaacccgt cgtacgtgtg tctccagaaa ccaccatcgt cagcaaggtg atgaaagcga
     2161 gccaggagct gccgctgttc gtgcgtggga tgtttcaagc gcagcgaatc ctcgagagtt
     2221 ttgacgagat tacgaaaccg gattgggtcc tgctggttgt cgggatgact ccgatgggtc
     2281 ggctgataca gtgaaaggca cggggcctca cgatcgacca gaagctcagc cagctcgggc
     2341 tgatggcgca gatccatgat cctacctcga ttctcaaggg gaacgaactg tccttcacat
     2401 ggaaagccat ggcgatggaa ggttccgcga gtggcggccg gaaatgcgta ctgctcgcac
     2461 gacggtcaat ggatgaccca gttcacagtc tctgccatcg agagggtatt ggcaagtccc
     2521 cagggcgttg ccctgggctc ttatgtgatt ggcccttcgg gccgaagttc aggaaaccgt
     2581 caggcgacct cgccggcagc cagggctgca tcaatctcag ctcccttggg gaagccaaat
     2641 tcgacccggt caagcaccag ggcggccgcg gcggacgacc gaagggcggc tgaccggatc
     2701 agaccatcgt cgatcacgcc cgaagcggca ttccaacctt gctcgagcaa ggtcgaaaga
     2761 agcgtcgcgg catacgactc gccggcccgg ttggtgtcgc gcggcggtcg ctggcgagga
     2821 aacgccggga cggtcaattt cccaggctca ccttgcgtgg tcgtgtaccg gatcgagctc
     2881 cccgctggtc catcggtcac caccaggatt gaaacttgcc aggcgacttc ctcgcggtct
     2941 tccaggctct cccactcgct gcgattacag ctcagcacgt tgattgaacc tgcaaagccg
     3001 gacagtggga attctcggtc gacgacattg cgcatcgccg gtgcgaaaaa cctcatgccg
     3061 gcgcccggtg cggccagcac ttgggccgcc aggcggttcg ggagtgaggc cacgacacgc
     3121 aggtcgcacg gccgcgccgc cttcgagtcg agcgaatcca gctcaagagc cgcgtggcag
     3181 ccgcgaaatc cgattggaag cttatcgcca aactcgccgc tcgtgatcag caaggtccag
     3241 tcagcgcagt gatcgaatac ccggaccgga tcgtgggcga tacccagctc ggccagcctg
     3301 gacgagatct cccggctcgt cgggtcggac tctgatccca gcgcgctaca gagttggcca
     3361 ccgagcgcgg ccgcgtagcc ggctcccatt cctcccaggt cattgtgcca ggtcagcccg
     3421 cgtaccgagc accggcccgt aacgcccggc ctgaccggac tggccaggcg gatctcaccc
     3481 gtcgggccgg gccagtcgac cgggagctcg accgcgattg catagccggc ggaatccatc
     3541 agctcgagcc gcggattggt accgaacttc caaactccat cgacgctctg atccagagga
     3601 ggccccagtt ccggggcgat caagggccga tccacgtaga ggacgcggtc cagataggcc
     3661 ggaccaaaaa ccagaa
//
LOCUS       DATAQL010000442         5930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4167027, whole genome shotgun sequence.
ACCESSION   DATAQL010000442 DATAQL010000000
VERSION     DATAQL010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4167027"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..835
                     /locus_tag="VHS97_07640"
     CDS             314..835
                     /locus_tag="VHS97_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3448112.1"
                     /translation="MPAPRHCLLIVDDEPDVCDSVADLLRREFRVLKANSAEEGYGIM
                     LQEEVHIVMSDQRMPQISGVELLTKVKARHPQAIRMLFTGFADLDSIIAAINQGHVFQ
                     FMKKPWQPEELVAAVRQAALEFDRLEALAHEREHLAGEVMELKHRVSALETEVQRLLA
                     QSGNPARPAGTAG"
     gene            991..2253
                     /locus_tag="VHS97_07645"
     CDS             991..2253
                     /locus_tag="VHS97_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3448113.1"
                     /translation="MEQRRHTLLVVDDEVDVLESLRHQFHRAYRVLTSVSGKQAIELL
                     LNEDVELILTDQRMPGMSGDQFLREARRLKPDAIRMLFTGYADIQAVISAVNEGHIFR
                     YILKPWDSVELEGIIHQGVEQYDLLAERRRLVAELQAANLELVQANLELARAGQLKTA
                     FIEVASHEFNTPITLVLGFTELLRLASPSRTDLEQEIIGQITTSGRQLARLVTNMLTL
                     LRAEDFRKTMERRPVQITDLIERVAEQMRPFTHVRRHVLITKLDDDLGAFEIDTDKLS
                     AVLINLLTNAIKFTPDGGTIELEARLSEPDVAEICVSDRGVGLEPQELRHLFQPFFTQ
                     FDPSRHSSGDFGFCKRGLGLGLSIAKQFVEMHGGQINAKSRDGGGTRVTVCLPRSANV
                     AEKQVDRDPPIGLPASSQSAGMAAGGAR"
     gene            2339..3109
                     /locus_tag="VHS97_07650"
     CDS             2339..3109
                     /locus_tag="VHS97_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3448114.1"
                     /translation="MPTALIVEDEPEANKLLAMLVQLRGYSTVSAYSGAEALEKVVTY
                     IPDVVFLDLMLPDRDGYDVCRALKASGTTSQVPVVIVTARIAAENRIESFRAGADDYV
                     PKPYTPDQIFEALAETHAQVQESALPPLEGEVVLDGRDDGQTLRQLARLRSSVQAHCG
                     LEPDEIERLSTAIKTIWSSADRWGRKAGIDRVATLAFSLSDDSLRLTVRDEGGWLANA
                     SELAPDPAWTVLADAGFDDIFYDREGRLLKLVKRFEKN"
     gene            3132..4175
                     /locus_tag="VHS97_07655"
     CDS             3132..4175
                     /locus_tag="VHS97_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448115.1"
                     /translation="MSSVQGDHRSEGSAFPVASLTDADRALLLALAVWGAPPQPENGA
                     AGLASDFRARLRRRLAPAGFPRVCDALLHESSPAIFREPQKARERLIKMHLASARVEP
                     AQVHPSWWVRALREESPAVQRLVTRAVPASLREPLQSGLLLDQEDLRSERAASSEVVN
                     WVLALWAERLVGGEVERSDDPPAIIALCRLSPRSGYRLCRLAGLCKLILAGLEKSDSG
                     RSSSRARHEWLEGRLPVNAELQAMARHDVEAGRSSRLPPRRHKARIGLVTFARLLADA
                     EPFRLRWALQHWPYPIAKLVRSLMPPTGRRSTSLLDWESLVLKTAWDRLNVEGRLSLD
                     PPDEHAADWSRLT"
     gene            4172..5506
                     /locus_tag="VHS97_07660"
     CDS             4172..5506
                     /locus_tag="VHS97_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX3448116.1"
                     /translation="MSPGISLSEPESHEALGSAPPAMADSDAGPFGMLPRVSRRQLGL
                     ERRLARWSADGGLPAPLDWLQQETGCSISLDKAEILAHTSGLRRAGLVAQLTAPRLAT
                     RLALGIEIPLAHAIVDRLLGFDRPFADSRLQLTPVEWGVWTFLILRALDSLASSEVPH
                     QQDRTGISGLLGPADLTLDRVGPDPFDPSGLGSIVTVRWSVRLGTLVSAVRLWLPDSV
                     VSLLLAPPSGPLFGQPRTTAQPASVDILAGSLGSAQRGELASGWRALTGFVTMSQGLK
                     RLRTGGVLPLADSRLTGTPASPGGPVDLILDLEGQDVRFRIPTRPVADSGGRLLRLET
                     RPMREPRPSDPITATKSENRPMSQPQVSSNLPSNAPTASPLDVPVTLTAELGRVNLTL
                     TQLADLKPGDVVELNRHSRAPIELTSNGRLVARGELILIDTDLGVRVTNVFL"
     gene            complement(5542..>5930)
                     /locus_tag="VHS97_07665"
     CDS             complement(5542..>5930)
                     /locus_tag="VHS97_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448117.1"
                     /translation="WWNLHHHARFYDEYPRTYRLWLVINPLELAIALGLPSVVWCITA
                     LLAPRKLPISVWSTFIVLALANLIGRNMGEVARLWLLYMPPLLVGAGYGCARWGPRPA
                     AIAWSIALLGVQTLALQSLIQVVYPV"
BASE COUNT         1125 a         1845 c         1832 g         1128 t
ORIGIN      
        1 ttgaaggcct ctcgcaatgc ctgattgccg tccggatcac aacaccctcg cttgtgcccc
       61 ggcggtcgca cgttgccaca tacaaaaata tgatgggtga acgcaggcat ggttgaccta
      121 tcaaaggctc gatggcggtg cagacccgtt tgaatcatga ttaaacacgg caagtgtaca
      181 gtagcaactt gtcgcccaaa ccgacagagg cgtcgaacca gcactttctc tgcgttataa
      241 tttaaggaac tcggtacgga ttctgctccc ggttccaaca tgtacgtctg aaaacgcgga
      301 gtgactcagc atcatgccag ctcccaggca ttgcctgctc atcgtcgacg acgagcccga
      361 tgtgtgcgac tcggtggccg acttgctgcg ccgcgagttt cgggttttga aggcaaatag
      421 cgcggaggaa gggtacggca tcatgctgca ggaggaagtg cacatcgtga tgtccgacca
      481 gcggatgcct cagatcagcg gcgttgagct tctgaccaag gtcaaggcgc ggcatcccca
      541 ggcgatacgg atgctgttca ccggctttgc cgacctggat tcgatcatcg cggcgatcaa
      601 ccagggtcac gtttttcagt tcatgaaaaa gccctggcag ccggaagagc tggtcgcggc
      661 agtgcggcag gcggcgttgg aattcgatcg gctcgaagcc ctggcgcacg agcgggaaca
      721 cttggcggga gaagtcatgg agctcaagca ccgggtctcg gcgctcgaaa ccgaggtcca
      781 gcggctcttg gcgcaatccg ggaatccggc tcgtccggcg ggcacggctg gctaaggtgc
      841 accgctcgat acagaactgc cgcggatcgg gttggttagc gtagaaaccg gcaatatcgg
      901 caaagtcaaa ggcagcagtt ctcgagtcag accaacgttt tctactgtat ttccagcgag
      961 atcctcaggg aatcagccgg agcaatcggg atggagcaaa ggcggcatac tctcctggtg
     1021 gttgacgatg aagtggatgt gttagagagt ctgcgccatc agtttcatcg cgcctatcgc
     1081 gtactcacgt ccgtctccgg taagcaggcc atcgagctcc tcttgaatga ggatgtcgag
     1141 ctgatcttga ccgaccagcg gatgcccggg atgtcgggtg atcaattcct cagggaggca
     1201 cggcggctca agccagacgc gatccggatg cttttcaccg ggtacgccga catccaggcg
     1261 gtcatcagcg ccgtcaacga agggcacatt ttccgctaca tcttgaaacc gtgggattcg
     1321 gttgagctcg agggcatcat tcaccaagga gttgagcagt acgaccttct ggccgaacga
     1381 aggcgtctcg tggccgaact gcaagccgcc aatctcgagc tggtgcaggc caacctcgag
     1441 ctcgcccggg ccggccagtt gaagaccgcc ttcatcgagg tggcaagcca tgagttcaat
     1501 acgccgatca ccctggtatt gggattcacc gagctattgc gcctcgcgag cccatcgcga
     1561 acggatctgg agcaagagat cattggccaa atcaccacca gtggacgaca actggctcgg
     1621 ctcgttacca acatgctcac cttgctccgc gccgaagact ttcgcaaaac catggaacgc
     1681 agacctgtcc agattaccga tctgatcgag cgggtcgccg aacagatgcg gccattcacc
     1741 catgtgcgac ggcacgtgct gattacgaag ttggacgacg acttgggcgc attcgaaatc
     1801 gataccgaca agctaagcgc ggtcctcatc aacttgctga ccaacgcgat caagttcaca
     1861 cccgacggtg gcacgatcga gctggaggcg cggctctccg agccagacgt ggccgaaata
     1921 tgcgtgagcg atcgcggagt gggcctggaa ccgcaggagc tccggcactt gttccagccg
     1981 ttcttcaccc agttcgaccc gagccgccat tcatcgggtg atttcggttt ctgtaagcgc
     2041 gggctggggc ttggcctgag tattgcgaaa cagtttgtgg agatgcatgg tggtcaaatc
     2101 aacgccaaaa gccgggacgg aggcggtacg cgcgtcacgg tttgcctacc gcgatctgca
     2161 aatgtcgccg agaagcaggt ggatcgcgac ccgccgatcg gtctgccggc gtccagccaa
     2221 agtgccggga tggcggctgg tggcgcgcgc taaaactagc catcagtctg ttccgagctc
     2281 aggctcgcgt tgtgccctgc cagcgactgt tcattcctcc ggttgacgag aacatcgaat
     2341 gccgactgct ctgatcgtcg aagacgaacc cgaggcgaac aagcttctgg cgatgctcgt
     2401 ccaacttcgc ggctactcga ccgtttcggc atatagcggc gccgaggctc tcgaaaaagt
     2461 cgtaacgtac attcccgatg tcgtattcct cgacctgatg ctccccgacc gcgacggcta
     2521 cgacgtctgc cgggcattga aggcatcagg gacgaccagc caggtcccag tggtgatcgt
     2581 caccgcgcgc atcgccgccg aaaaccggat cgagagcttc cgagccggcg ccgacgacta
     2641 tgtccccaag ccgtatactc ctgaccagat cttcgaggcg ctcgcagaga cccatgccca
     2701 ggtgcaagaa agcgctcttc ctcccctcga gggcgaggtt gttctcgacg gccgtgacga
     2761 cggccaaacc ctgcgccagc tggcacggtt gcgcagctcg gtgcaggctc actgcggtct
     2821 cgaacccgac gagatcgagc ggttgagcac ggcgatcaag acgatctggt cgagtgccga
     2881 tcggtggggg cgcaaggccg gtatcgatcg cgtggcaacc ctggcgtttt ctctgtccga
     2941 cgacagcctc agattgaccg tccgtgacga gggaggttgg cttgcaaatg caagtgagct
     3001 ggccccagac ccggcatgga ccgtcctggc tgacgctggc ttcgacgata ttttctacga
     3061 tcgcgaggga cgtttgttaa agctcgtcaa acgattcgag aaaaactagc tcacggtcga
     3121 gggcgccacg tatgagttcc gtccaaggcg atcatcgatc tgaaggctcc gcgttccctg
     3181 tggcgagcct caccgacgcc gatcgcgcgc tccttctcgc cttggccgtg tggggagcgc
     3241 cgccacaacc cgagaacggg gcggctggcc tcgcatctga tttccgcgcg cgacttcgcc
     3301 gtcggcttgc cccggccggc tttcccaggg tttgcgacgc tctcttacac gagtcgtcgc
     3361 cggcaatatt tcgcgagccg caaaaagcga gggagcggct catcaagatg catctggcgt
     3421 cggcgcgcgt cgagcctgcg caggttcacc ctagttggtg ggttcgtgcc ctccgggaag
     3481 agtccccggc cgttcagcga ctggtcacca gggcggtgcc tgcatcattg cgcgagccgc
     3541 tgcaaagcgg actcttgctc gaccaagaag accttcgcag cgagcgggcg gcctcatccg
     3601 aggtcgtcaa ctgggtgctc gcactctggg ctgagcggct cgtggggggc gaggtagagc
     3661 gatccgacga cccgcctgcc atcatcgccc tttgccgtct gtcgccccgc tccggctacc
     3721 gcctttgccg gttggcgggt ctgtgcaaac tgatactggc cggccttgaa aaatcagact
     3781 caggtcgttc gtcaagccgt gcgcggcacg agtggctcga aggccgtttg ccggtgaatg
     3841 cggaacttca ggcaatggcg cggcacgacg tagaggcggg tcgttcttcc aggcttccgc
     3901 cgcggcggca taaggccagg atcggactgg tgaccttcgc ccgactgctc gccgatgccg
     3961 agccttttcg cctccgctgg gcgttgcagc actggcctta cccgatagcc aagctcgtcc
     4021 gttccttgat gcctcccact ggcagacgtt ccacttcgct gttggactgg gaatcactgg
     4081 tcttgaaaac ggcctgggac cgcctcaacg tggaggggcg actatcgcta gacccgccag
     4141 acgagcatgc ggctgactgg agtcgtttga cgtgagcccg ggaatcagcc tgtcggagcc
     4201 agaatcgcac gaggcactgg gaagtgcgcc gccggccatg gccgacagcg atgccggccc
     4261 gtttggaatg ctgccgcgcg tctcgcgccg gcaactcggt ctggaacgcc ggctcgctcg
     4321 ctggagcgcg gacggcggac ttcccgctcc gctcgactgg ctgcaacagg agaccggctg
     4381 ctctatttcg ctcgataaag ccgaaattct cgcgcatacc tcgggattgc gccgcgccgg
     4441 tttggtcgcc cagctgacag ccccaaggct ggcgacgcgt ctggctctgg gcatcgagat
     4501 tccactggcc cacgccatcg tcgatcgcct gctcggtttc gatcgcccct tcgccgactc
     4561 tcggctccag ctcacgcccg tggaatgggg tgtgtggacc ttcctgatcc tgcgggcact
     4621 cgattcgttg gcttcttcgg aggtaccgca ccagcaagac cgcaccggaa tctcgggtct
     4681 cctggggccg gcggacctga cgctcgaccg ggtcggcccc gatccgttcg atcccagcgg
     4741 actggggtcg atcgtgaccg tgcgctggtc cgtccgtctc ggcactctcg tgtccgctgt
     4801 gcggttgtgg ctgccggact cggtggtcag ccttttgttg gcccctccgt cgggacctct
     4861 cttcggccag ccgagaacga cggcgcagcc ggcgagcgtt gatattctgg ccggatctct
     4921 gggctcggct cagcgcggtg agctggcgag cgggtggcgc gcccttactg ggtttgtgac
     4981 catgtctcag ggactgaagc gactccggac cggaggcgtc ttaccgctgg ccgactcgcg
     5041 actcactggc actcccgcca gcccgggcgg tccggtcgac ctgatactcg acttggaagg
     5101 tcaggatgta cgattccgaa tcccgactcg tcctgtggcc gactcgggtg ggcggttgct
     5161 ccgacttgaa acccgtccca tgcgcgagcc tcgaccaagc gatccaatca cggcgacaaa
     5221 gagcgagaat agacccatga gtcaaccgca agtctcgtcg aatcttccgt cgaatgcgcc
     5281 caccgcgtct ccactcgacg tgccggtcac cttgaccgcc gagctgggcc gcgtcaacct
     5341 gaccctcacc cagctcgccg acttgaaacc tggcgatgtc gtcgagctga accgacatag
     5401 ccgcgcgccg atcgagctca cgtccaacgg cagactcgtc gcccgcggcg agctgatcct
     5461 gatcgatacc gacctgggcg tgcgcgtgac caacgttttc ctctgagtga gctgctcggc
     5521 gcgtgagact cgatctgccg tctacaccgg ataaaccacc tggatcaacg attgcagcgc
     5581 cagtgtctgc acgccaagca atgcgatcga ccaggcgata gcggccggcc ggggccccca
     5641 gcgagcgcac ccgtaccccg ctccgaccag gagggggggc atgtaaagca accacaatcg
     5701 ggcaacttcg cccatattgc ggccgatcag gttcgcgagc gcgagaacga tgaatgtcga
     5761 ccataccgag atcggcagct tgcgcggcgc gagaagtgcc gtgatgcacc agaccactga
     5821 cggaagccca agggcgatcg ccagctcgag cggattgatc acaagccaga gccgataggt
     5881 gcgtggatat tcgtcataga atcgcgcgtg gtggtgcaag ttccaccagc
//
LOCUS       DATAQL010000443         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1725119, whole genome shotgun sequence.
ACCESSION   DATAQL010000443 DATAQL010000000
VERSION     DATAQL010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725119"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VHS97_07670"
     CDS             complement(<1..238)
                     /locus_tag="VHS97_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX3448118.1"
                     /translation="MTLPSSESQSLTFDSHKTNRLAGETSPYLLQHAHNPVDWYPWGS
                     EALAKAKSDNKPIFLSIGYSACHWCHVMERESFEN"
     gene            complement(380..694)
                     /locus_tag="VHS97_07675"
     CDS             complement(380..694)
                     /locus_tag="VHS97_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX3448119.1"
                     /translation="MSVALKYPHREKPEWEPARLERLPRIRVAQIAMDYLAHGWSVDE
                     MWHQHPYLTLAEAHAAMAYYFDHQADIDREINEEIDQVERDQSRAPRSATYHRLRAQT
                     LP"
     gene            complement(744..2453)
                     /locus_tag="VHS97_07680"
     CDS             complement(744..2453)
                     /locus_tag="VHS97_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX3448120.1"
                     /translation="MKSQLLASSLVVALVLAVSPVHALAEDEHVKLAATARFDVMADA
                     HVGVLDNGKVVAGNGTIARMNWVPESEQPRGYTVNFPVTHRSWKPLAVHFTPVHDGTV
                     TLSLMGPWEQASPGVVYRQEVLWDDLSADGAALTDGGFESRRGARAGGWQSGGGSVVE
                     QSTGVAAVEGTHYARTWHNQTLSTSFKVVGGRPVTIRVSARAVRPEGLIEMKRLTSRT
                     TPAHLAARRFRRGANLGNGLETPPGQDWGGHYSPTDLRIMRAEGFDHVRIPAGWHHYT
                     GPGPEFRIRPEFFGRVDELVNAGLREGLAVLINIHHFDDFTSHPKEQTPRFLAIWRQI
                     AEHYSRAPEGLALELLNEPKDAATTEVINPIFAEAIRQIRRIDPKRTIFLGPSKWNSI
                     SELAKLQLPDDDDNLIVTVHNYEPFYFTHQGATWAGPDTKVTGILFPGPPAKPLSPDP
                     SLKVNSGVLNWIKAYNTQPTATNPSSAHAFQGAVDQAREWSEYYGRPIHVGEFGCFTT
                     ADSVSRANYYRALRESAETAGFGWAIWDWKAGFHYWNEKTNKAEPGMHDALFGTSSSR
                     TIH"
     gene            2700..3857
                     /locus_tag="VHS97_07685"
     CDS             2700..3857
                     /locus_tag="VHS97_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX3448121.1"
                     /translation="MSAKSSRREFLGHGVSALGLLAWAGHQPGIASAADDAAKDGATA
                     SESARKLTDRGVAFLRPRQDAKGGWSTQREPGVTALVVTALLRSGQVAPGEPAVIKAL
                     AYLEGFIGPKGGLSEAPHANYSTSIALLAFREANAHGRYDRTIKAGQEFLKTMQWDES
                     EGKTREDSFYGGAGYGGSNSRPDLSNTTFFIEALRETGLPADDPNLKKAIVFVSRCQN
                     LKSEFNDQAWASKINDGGFVYTAANGGQSMAGEAAGGGLRSYASMTYAGLKSMIYAGL
                     VRDDPRVKAAVTYITNHYTLDENPGLGQQGLYYYYHTFAKTMALLGEPTLTDAKGVSH
                     DWRAELVAELSKRQLPEGGWVNPADRFMEGDPNLVTAYALLALAYTRPKAK"
     gene            complement(3901..>4192)
                     /locus_tag="VHS97_07690"
     CDS             complement(3901..>4192)
                     /locus_tag="VHS97_07690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX3448122.1"
                     /translation="DLPDAVAAATSSLLPRLCRAIRAATGAEGLNVIVNNGQVAGQTI
                     DHCHWHIIPRFTNDAVNWPWPHSPYVGDELGQLKYRIERELAADLLQGKPES"
BASE COUNT          793 a         1194 c         1335 g          870 t
ORIGIN      
        1 cattttcgaa gctctcacgc tccatcacat ggcaccaatg acaggcggaa tagccgatcg
       61 aaaggaaaat cggcttatta tcggacttcg cttttgccag cgcctcgctg ccccagggat
      121 accaatcgac cgggttgtgc gcatgctgga gcaagtatgg gctggtctcg ccggccaggc
      181 ggttggtctt gtgcgagtca aacgtcaagg attgcgattc ggatgagggt aaagtcatga
      241 gagaggatct ttcgcgcgtt gagttccgtt tgaatcgttt ttggggaatg atttgcaatc
      301 tagtgcgcgt tcattctagt gcgcgttcat ttactctttg gtgcagcctg gaatgccgcg
      361 ttcaagttca aaacggacat cagggcagag tctgcgcacg gaggcgatgg taagtcgccg
      421 atcggggcgc tcgcgattga tcccgttcga cctggtcgat ttcttcatta atctcccggt
      481 caatgtcggc ttggtgatcg aagtagtagg ccatcgccgc gtgcgcctcg gcgagggtga
      541 gatagggatg ctgatgccac atttcatcga ccgaccagcc atgggcgaga taatccatcg
      601 cgatttgggc cacgcggatt cgcggcaaac gctcaagccg agcaggttcc cattcgggct
      661 tttctctatg tggatacttg agagcgacgg acatcgccca agactccttc gctacacgtc
      721 tactattcgt ccagaatgag cgttcagtgt attgtacggg atgacgacgt tccaaataga
      781 gcgtcgtgca taccaggctc ggccttgttt gtcttctcgt tccagtaatg gaaaccggcc
      841 ttccagtccc agatggccca accaaacccc gctgtctcgg ccgattcacg caaggcgcgg
      901 tagtagttgg cccgtgatac ggaatcagcc gttgtaaaac aaccgaactc acccacatgg
      961 atcgggcggc cgtagtattc cgaccattcc ctggcctggt cgacggcgcc ttggaacgcg
     1021 tgcgcgctgc tgggattggt ggcggtgggt tgcgtgttgt aggccttgat ccagttgaga
     1081 accccggaat tgaccttgag actgggatcg ggcgaaagtg gcttggccgg tggaccggga
     1141 aagaggatac cggtcacttt cgtgtcgggt cccgcccagg ttgctccctg atgagtgaag
     1201 taaaacggct cgtagttgtg tacggtcacg atcaggttat cgtcatcatc gggcaattgc
     1261 agcttggcta actcggagat gctattccac ttactcggtc cgagaaatat ggtgcgcttg
     1321 ggatcgatcc ggcggatttg tcggatggcc tcggcaaaga tgggattgat gacttcggtc
     1381 gtggcggcgt ctttcggctc gtttaggagc tcgagtgcaa ggccctcggg cgctctcgag
     1441 taatgctcgg cgatctggcg ccagatcgcc aggaatcggg gcgtctgttc cttcgggtgg
     1501 gatgtgaaat catcgaagtg gtggatattg ataagaacgg ccaggccctc gcggagaccg
     1561 gcgttcacca gctcgtcgac ccgtccgaaa aattcggggc gaatgcggaa ctctggcccc
     1621 gggccggtgt agtggtgcca gccagcgggg atgcggacgt ggtcaaaccc ctcggcacgc
     1681 ataatgcgga ggtctgtcgg cgagtaatgg ccgccccagt cctgacccgg gggcgtttcc
     1741 agtccgttgc cgaggttggc ccctcgccgg aatcgtcgcg cagcaagatg tgccggagtg
     1801 gtgcggctag tgagccgctt catctcgatc aggccctccg ggcggaccgc ccgtgcgctg
     1861 acccggatcg tgaccggccg gcctcccaca accttgaacg atgttgaaag cgtctggttg
     1921 tgccatgtcc gggcgtagtg cgttccttcg acggctgcga cgccggtgga ttgttcgacc
     1981 acgctccctc cgccgctttg ccagccgccg gcccgagccc ctcgccgcga ttcgaagcca
     2041 ccgtcggtca gggccgctcc atcagcactc aggtcatccc acaaaacctc ctggcggtag
     2101 accaccccgg gcgacgcctg ttcccacggg cccatcaagc tcaaggtcac ggttccgtca
     2161 tgcaccggcg tgaaatgcac ggccagcggc ttccagcttc ggtgcgtaac gggaaagttg
     2221 accgtgtatc cgcgaggctg ttcggattcg ggcacccagt tcatgcgcgc gatcgtcccg
     2281 ttgccggcga cgaccttgcc gttgtcgaga acgcccacgt gcgcatcagc catgacgtcg
     2341 aatcgcgccg tggcggccag cttgacgtgt tcgtcctcag cgagggcgtg gacgggcgaa
     2401 acggccagga ccaaggccac aacaaggctg ctagcgagca attgactctt cataacgaat
     2461 catcttctat agatggagat actcactcat cggcaccgtc gggcgtcgtc tgttcacctt
     2521 tagtgtctcc gtgcctcgtt tttgacgcaa ggggcaagga attgccgacg tcttagtcac
     2581 cggccgttat aatgaaagca atgaacagac aatccgccga gtggttacga gtcaccgcac
     2641 agcactgagc ggcattggtg tcgcggcaca ggtgtcctcg gaagaaagga tcgatcatga
     2701 tgtccgcaaa atcgtcacgc cgcgaattcc ttggacacgg agtctctgct cttggcttgc
     2761 tggcgtgggc agggcatcag cctggcattg cttccgccgc ggacgatgcg gccaaggatg
     2821 gggcgacggc ttctgagtcg gcgaggaagt taacggatcg cggggtcgcc ttcctgcggc
     2881 cgcgtcagga tgccaaaggg ggatggtcga cgcagcgcga gcctggagtt acggccctgg
     2941 tcgtgacggc gctgttgcgc tcgggacagg tcgcgcccgg cgagccggcg gtgattaaag
     3001 ctctggccta tctcgagggc tttatcgggc ccaagggcgg gttatccgag gctccccacg
     3061 ccaattactc gacttcgatc gcgcttttgg cgttccggga agcgaatgcg cacggccgtt
     3121 acgaccgtac gatcaaggcg gggcaggaat tcttgaagac gatgcagtgg gatgagagcg
     3181 agggcaagac gcgcgaggat tcattttacg gtggcgccgg ctatggcggc tcgaacagcc
     3241 ggcccgacct gtccaatacc acgttcttca ttgaggcctt gcgggagacc ggtttgcccg
     3301 ccgatgatcc caacttgaag aaagcgatcg ttttcgtttc gcgctgccag aacttgaaga
     3361 gcgagttcaa cgatcaagcg tgggcgtcga agatcaacga cggcggattc gtgtatacgg
     3421 ccgccaatgg cgggcagagc atggccggcg aggcggccgg cggcggtctc cgctcgtacg
     3481 caagcatgac ttacgccggt ctcaagagca tgatttacgc ggggctcgta cgcgacgacc
     3541 cacgcgtcaa ggcggcggtg acttacatca ccaatcacta cacgctcgat gagaatcccg
     3601 ggctcggcca gcaagggctt tattactatt accatacctt cgccaagacg atggctctct
     3661 tgggcgagcc gacactgacc gatgccaagg gggtatcgca cgactggaga gcggagctcg
     3721 tcgcagagct ttcgaaacgg cagcttcctg aaggtggttg ggtcaacccg gccgaccgct
     3781 tcatggaagg cgaccctaac ctggtgaccg cctacgctct gttggcgctg gcgtacacgc
     3841 ggccgaaggc gaaatgactg gccgcgacga ggccggtgac aagcaagcgc atcgtctggc
     3901 ttaactctcc ggcttgccct ggaggagatc agcagctagc tcgcgctcga tgcggtattt
     3961 cagctgccca agctcgtcgc caacgtaggg gctatgaggc cacggccagt tcactgcgtc
     4021 gttggtgaag cgggggatga tgtgccagtg gcaatgatcg atggtttggc ctgcgacctg
     4081 gccgttgttg acgatcacgt tcagtccttc ggcccccgtt gccgcgcgga tggcccggca
     4141 cagtcggggc aagagcgagc tcgtggccgc ggcaacggcg tccgggaggt cg
//
LOCUS       DATAQL010000444         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_45247, whole genome shotgun sequence.
ACCESSION   DATAQL010000444 DATAQL010000000
VERSION     DATAQL010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45247"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..512)
                     /locus_tag="VHS97_07695"
     CDS             complement(78..512)
                     /locus_tag="VHS97_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448123.1"
                     /translation="MCDDNDQTKPRPKEFDFSAFPPNTVFHERRTGRERRDAGSLGQK
                     PDAVAPRPVAIEHRTKKDRRRRIDPTTFEKQYTVDEMEFMNAMQRFKESTGRSFPTYA
                     EVLKVAATLGYRRVVVDDAPSWDDSIDDEPCPAESSSINVLA"
     gene            1082..>1510
                     /locus_tag="VHS97_07700"
     CDS             1082..>1510
                     /locus_tag="VHS97_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448124.1"
                     /translation="MSRTELLSRGAGFLMLGGVLLSGVSAFGADEPPLSNQLTELGRQ
                     ALAQGSSKAAKNFFEKALTLDPSNPAATRGLKDAESAERGVIKVAFQDEPKPDQPPAG
                     QDAKPDQPPAGAAPATPPPPAPATDRATIEQSEAAENIARQ"
BASE COUNT          288 a          437 c          464 g          321 t
ORIGIN      
        1 ttggctggac ccgcgctcgt cgacatttcg gcgcggtgaa caaaccgtcg atcgacctct
       61 gtcggcacgt ccgtcactca agcaagcaca ttgatcgatg acgattcggc cgggcacggc
      121 tcgtcgtcga tcgaatcatc ccaagacggg gcatcgtcaa caaccacgcg acgatagccg
      181 agcgtagcgg caaccttgag cacttcagcg taggttggga agctcctgcc ggtgctctcc
      241 ttgaatcgct gcatcgcgtt cataaactcc atttcgtcga ccgtgtattg tttctcaaag
      301 gtcgtgggat cgatgcgccg ccgccggtcc ttcttggtgc ggtgctcgat cgcgacggga
      361 cgaggggcga ccgcgtccgg tttctggccg agcgatccgg cgtcgcgccg ttcgcgcccc
      421 gttcgtcgct catgaaatac cgtgttgggc ggaaaggcgg aaaaatcgaa ttccttgggc
      481 cttggtttcg tctgatcatt gtcgtcgcac atgcgttgaa atctcggatt tcctgggtcg
      541 agcttgttct cgcaatgaaa aacggcgcgt gaatcgtcgc ttatggcgga tcatcaatcg
      601 ccgccggcca cgcattcggc ccgctcgcgt ggacagccgg ctttgaaagc gacagccaag
      661 agacgtttgt cggcgcctcc cacttcaaaa ccactaggac gctaacgcag gtgtgtcaag
      721 gagtatgact ccggtggcga tgaaggtagc aatctctggt tgcgccgttt ggccggtggg
      781 cgagtgtgtg gcataatgaa ccaagagcac gatggaatcc cgcggcggca ccgcacgcgg
      841 gtgagcttga gcaccttggt tcggcatttg cgtccatcaa tccggtacca tttccttttc
      901 acgaaatgcg cctgggggat tttgagttcg tcgcgggttg gcctcgtggg acgctttgtg
      961 agcggaattg acggatgggc ttgtagttga gtcttgagtt gatgtagctt attcttagga
     1021 acatcgagcg cctaagccgg ttttcgagcc ggtcaaatct ttctggtgga gagcaccaaa
     1081 catgtcccga accgagcttc tatcgagagg tgcggggttc ctgatgctgg gtggcgtgct
     1141 gctgtctggc gtctcggcct tcggtgccga cgaaccgccg ttatcaaatc aattgaccga
     1201 gctaggccgc caggcgcttg cacaggggtc gagcaaggcc gcgaagaact ttttcgagaa
     1261 agcgctgacg cttgatccgt cgaatccggc ggcaacgcgt ggcctcaaag acgccgagag
     1321 tgctgagcgt ggcgtcatca aggtcgcttt tcaggatgag cccaagcccg atcaaccccc
     1381 agccggtcag gatgccaagc ccgatcaacc cccagccggc gcggcgccgg ctacgcctcc
     1441 gccccctgct cctgcgacag acagagccac gatcgagcaa agcgaggcgg ccgagaatat
     1501 cgcccgccag
//
LOCUS       DATAQL010000445         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2486883, whole genome shotgun sequence.
ACCESSION   DATAQL010000445 DATAQL010000000
VERSION     DATAQL010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486883"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VHS97_07705"
     CDS             complement(<1..623)
                     /locus_tag="VHS97_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX3448125.1"
                     /translation="MHKKPSVRLVAYILAVLAPALTLLALWPLPAALGDQVLYTGFFP
                     AVLIAAYLGGLGPGLLATVISALAATFFLVEPLYSFAIHSGSDAVALAVFLLVGVIIS
                     GLGESLQLARRRIVTVERQRAEEALRETEERFRGTFEHAAVGIAHVDAESRCLRANAK
                     MCEILGYTGAELVGKTVPEVTHPDDLGPNFFWFGALMRGELANFTME"
     gene            1029..>2128
                     /locus_tag="VHS97_07710"
     CDS             1029..>2128
                     /locus_tag="VHS97_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3448126.1"
                     /translation="MAHLLLIDDDPAVIQEQVRQTFPAPAHRVEIARTGAAGLDHVRA
                     DLPDVILLDLRLPDQSGLEVYQQIRRIDARIPVIFITMAKGADAAIEAMKHGAYDYLF
                     KPLGLEQLRRVVDEALDVARRMRQPAIVAETPPDPDVEGAILGGCPAMCEVYKAIGHV
                     AGQDVTVLITGESGTGKELVARAIYQHSSRAQSPFLALNCAAIPETLLESELFGHEKG
                     AFTGADRRRIGKFEQCNHGTIFLDEVGDMPLALQAKLLRVLQEQSFERVGGNETVRTD
                     VRLIAATHRDLKAWLEDGRFRPDLYYRLSAFSVDLPPLRERSDDLPMLVRHYLSRLGR
                     ELGREVVEVAPAAMDRLREYSWPGNIRELQNVL"
BASE COUNT          416 a          674 c          649 g          389 t
ORIGIN      
        1 ttttccatcg tgaagttggc cagctcgccc cgcatcaagg ctccgaacca gaagaagttg
       61 ggccccaggt catcggggtg cgtcacttct ggcacggtct tgccgactag ctccgcgccg
      121 gtatagccga ggatctcgca catctttgcg ttggcgcgca agcaacggct ctcggcgtcg
      181 acatgagcga tgccgacggc cgcatgctca aacgtgccgc ggaagcgttc ctcggtttcg
      241 cgcagggcct cctcggcccg ctggcgctct accgtcacga tgcggcgccg ggccagttgc
      301 agggattcgc ctaagccgct gatgatcaca ccgaccagca ggaagacggc gagagcaacg
      361 gcgtcggacc cgctgtggat cgcgaaggaa taaagcggtt cgacgagaaa aaaggtcgca
      421 gctagagcgc ttattacagt cgctaacaaa cccggcccga gcccgcctag gtaggcggcg
      481 attaatacgg ccggaaagaa gcccgtgtaa aggacttggt ctccaagcgc cgccgggagc
      541 ggccacagcg ccaacagcgt gagggctggc gccaaaaccg ctagaatgta ggcgacgagc
      601 cggacgcttg gttttttgtg catgccaacc tcatgtgacg cgtacggcca ccgttgctct
      661 tggcgcctac cgcaaagcct ttgctattga gatatcgatt gtgcttaaaa actcttcggc
      721 ttcgtaagtg atcatcgtcg ggattgttcg agatagggcc gaggagaaaa taacttggcg
      781 ccattaggtc cgccgagtcc tgccgaaata gtctgcaaca gcgacgggcc tgcttttcga
      841 aagtaatcga cccccttcgc agcgacaccg ctgccccaat caccgatgcg aaaccggtgc
      901 caaaagaatt cgcgggtcgt tatgctatcg ttccatgtct ctccctcaag gctggacagg
      961 ccgcgtcaac gccttgacaa tgagcgggtt ctgtggccgt cccctgactt agcgggagca
     1021 agcaatccat ggcccatctg ttgctcatcg atgacgaccc ggccgtgatc caagagcaag
     1081 tgcgccagac gttccccgct cccgctcacc gggtcgagat cgcccgcacc ggggctgcag
     1141 ggctggacca cgtccgtgcg gacctgcccg acgtgatttt gctcgatctc cgcctgcccg
     1201 accaatccgg cctggaggtc taccagcaga ttcgccgcat cgacgcgcgc atcccggtca
     1261 tcttcatcac catggccaaa ggcgctgatg cggccatcga ggcgatgaag cacggcgcct
     1321 acgactacct gttcaaaccg ctcggcctcg aacagttgcg gcgagtagtc gacgaggcgc
     1381 tggacgtggc ccggcggatg cgccagccgg ccatcgtcgc ggaaaccccg ccggaccccg
     1441 acgttgaggg cgctatcctg ggcggctgcc cggccatgtg cgaggtctac aaggccatcg
     1501 gccacgtcgc cggccaagac gtcactgtgc taatcaccgg cgaaagcggt acaggcaaag
     1561 agctcgtcgc ccgcgcaatc taccagcaca gttcccgggc tcaatcgcca tttctggctc
     1621 tcaattgcgc cgccataccc gagactctgt tggagagcga gctcttcgga cacgaaaagg
     1681 gtgcctttac cggagctgac cgccgccgca tcggcaagtt cgagcaatgc aaccacggta
     1741 ccatttttct cgacgaagtc ggagacatgc cgttggccct gcaggccaag ctgctgcgtg
     1801 tcttgcagga gcagtcgttc gaacgcgtcg gcgggaacga gactgttcgg accgacgtgc
     1861 ggctgatcgc agcgacgcac cgcgacctga aagcctggct ggaggacgga aggtttcggc
     1921 cggacctgta ttaccggctg agtgccttca gcgttgacct tcccccttta cgtgaacgaa
     1981 gcgacgactt gccgatgctc gttcgccatt acctgtcgcg gctcggccgg gagctggggc
     2041 gggaggtcgt cgaggtcgcc ccggcggcga tggaccgttt gcgcgagtac tcctggcccg
     2101 ggaacatccg cgagttgcaa aacgtgct
//
LOCUS       DATAQL010000446         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4014090, whole genome shotgun sequence.
ACCESSION   DATAQL010000446 DATAQL010000000
VERSION     DATAQL010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4014090"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VHS97_07715"
     CDS             <1..1409
                     /locus_tag="VHS97_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3448127.1"
                     /translation="LELLTYVRRKHREVPVILLFPRLHPERAKEALRLGAMAVLKYPV
                     PAAELRAAVLQALELCDAKAPSEPAGQPPAATVASVTAKPATPSTTVIHDGQPPSAPR
                     PMAINPLLSTGNSGTAGDVGGPAPSGSSLPRIEQLTREIGLIGNDPGWRQVVDLAATI
                     ASTRTSVLIVGEPGTGKSLLARLIHAIGPNSERPFITVEASALAAELSNHEPADAVYP
                     PRGNDPQAWTSKVAQARGGTLYLDEVAAMPLEFQLNLLRDLHFRDYEASAGHPLSSGD
                     ARFLMCTSENLPALIEQGRFRQELYHRISVISLMIPPLRHRGTDVELLAESFRAHYSR
                     EFHKSVVGFTRDALEVLQKHDWPGNVRELEAAVQRAVALCSGPRITSSHLAPILNHNR
                     QPRSGGGTMPRPHLKMGVRPLKEALEEPEKRIIIQALQAFNWNRQETAKVLDINRTTL
                     YKKMKKYNLLIDEPVWAN"
     gene            complement(1415..>2831)
                     /gene="ilvB"
                     /locus_tag="VHS97_07720"
     CDS             complement(1415..>2831)
                     /gene="ilvB"
                     /locus_tag="VHS97_07720"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX3448128.1"
                     /translation="HVIGTDAFQETPMVEVSRAITKHHYLVQDAKDIARIVREAFHLA
                     STGRHGPVLIDMPKDVQNTVVANPAYDVAMDLPGYRLPPAPSGRKIDEVIEAIRASSR
                     PVIYCGGGVVASGAAEELREFAAKTGIPVAMTVHGLGAIPSDHYLSLNMLGMHGTVYA
                     NYTVNEADLLLAFGVRFDDRVTGKLSEFAKHGRIVHVDIDASEINKNKVAHIPVHTNV
                     KTFLRAINPLVTAGDWRDWHQQIDEWRASDPMTYDQRDDVIMPQYVLEQFSKLAKGDF
                     IMTTGVGQHQMWAAQWTKFKHPRTWISSGGLGSMGYGLPAAMGAQAAFPDALVVDIDG
                     DGSFVMNIQELATVHCEKLPVKVIVLNNQHLGMVVQWEDRFYESNRAHTYLGPIHHPE
                     ATGQGESEIPESLYPDFVEIARGFGVKARHVQQKAEVVDALAEMIAHPGPYVLDVMVP
                     YQEHVLPMIPAGMTVRDIIKS"
BASE COUNT          537 a          899 c          829 g          566 t
ORIGIN      
        1 ctctggaact cttgacctac gtgaggcgca aacatcgaga ggttcccgtc attctcttgt
       61 ttcctcgcct tcatcccgag cgggcaaagg aggcgctgcg attaggcgcg atggccgtcc
      121 tcaaatatcc cgtccctgcg gccgagcttc gtgccgccgt gttgcaagcg ctcgagctgt
      181 gcgacgccaa ggcgccgtca gagcccgctg ggcagcctcc agccgccacc gtggcttctg
      241 tcacggccaa acctgcgacc ccgtcgacga ccgtaatcca cgacgggcag ccgccgtccg
      301 cgccgcgccc gatggcgatc aatcctcttc tgtcgactgg caattcaggt acagcaggcg
      361 atgtgggcgg accggcgccg tcagggtctt cgctcccccg tatcgagcaa ctcactcgcg
      421 agattggttt gatcggtaac gacccgggct ggcggcaggt cgtcgacctg gcggcgacga
      481 tcgcttcgac ccgcacctcg gtcttgatcg tcggcgagcc gggaaccggc aagtcgctcc
      541 tggcacgctt gatacacgcg attgggccca actccgagcg gcccttcatc accgtggagg
      601 cctcggcgct ggccgcagag ctgtcgaacc atgagcctgc tgatgcggtt tatccgccca
      661 ggggtaatga cccccaggcg tggaccagca aggtagccca ggctaggggc ggcacgctct
      721 atcttgacga agtggccgcc atgcccctcg agtttcagct caatctgctt cgcgacctgc
      781 attttcgcga ctacgaggca agcgccggcc atcccttgtc ttcgggagac gcccggtttc
      841 tcatgtgcac gagtgaaaac ctccccgccc tgatcgagca gggccgattt cgccaggagc
      901 tctatcaccg gatcagcgtc atcagcctca tgatcccccc cttacggcac cgcgggaccg
      961 atgtcgagct tttggccgaa tcgttccggg ctcactattc ccgcgaattt cacaaatccg
     1021 tcgtgggatt cacccgcgat gcactcgaag ttttgcaaaa acacgactgg ccaggcaacg
     1081 tccgcgaact tgaagccgcc gttcagcgcg ccgtcgccct ctgcagcggt ccccgcatta
     1141 cttcaagcca tctggcgccc atactcaatc acaatcgcca gccacggtcg gggggcggaa
     1201 ccatgcccag acctcacttg aagatgggcg ttcgaccctt gaaggaagcc ctggaagaac
     1261 cggaaaaacg gatcatcatc caggctcttc aagcgttcaa ctggaaccgc caagagacgg
     1321 ccaaagtgct cgatatcaat cgcaccactc tgtacaagaa aatgaaaaaa tataatctgc
     1381 taatcgatga gcccgtctgg gccaattgat aaagtcagct cttgataatg tcgcgcaccg
     1441 tcatgcccgc cgggatcatc ggcaggacgt gttcctgata gggaaccatc acgtcgagta
     1501 cgtacggacc gggatgcgcg atcatctcgg ccagggcatc gacaacctcg gccttttgtt
     1561 gaacatgccg agccttgacg ccgaacccgc gagcaatctc gacgaagtcg ggatagagcg
     1621 actcggggat ttcactctcg ccctggccgg tcgcctcggg atggtgaatg ggccccaggt
     1681 aggtgtgggc gcggttgctt tcgtagaatc ggtcttccca ctgtaccacc atgcccaggt
     1741 gctgattgtt gagcacgatc accttgaccg ggagcttttc gcaatgcacg gtggccagct
     1801 cttggatatt catgacgaaa ctgccgtcgc cgtcgatgtc gaccacgagc gcatcgggaa
     1861 aggccgcctg cgctcccatg gcggcgggta agccatagcc catcgacccc agcccgccgg
     1921 agctgatcca ggtgcgggga tgcttgaact tggtccactg cgcggcccac atctgatgct
     1981 ggccgacgcc cgtcgtcatg atgaaatcgc ccttggccag ttttgagaac tgctccaaaa
     2041 catattgagg catgatgaca tcgtctcgct ggtcgtatgt catcgggtcg ctggcacgcc
     2101 actcgtcaat ctgctggtgc cagtcgcgcc aatcgccggc cgtgacgagc ggattgatcg
     2161 cccgcaggaa agtcttgaca ttggtgtgaa cggggatatg agcgaccttg tttttgttga
     2221 tctccgacgc atcgatatcg acgtgcacga tccggccgtg cttggcgaat tcgctgagtt
     2281 tgccggtgac gcggtcgtcg aaccgcacgc cgaaggccag tagcaagtcg gcctcgttca
     2341 cggtgtagtt tgcgtaaacg gttccgtgca tccccaacat gttgagcgac aggtagtggt
     2401 cgctggggat ggcccccagg ccgtggacgg tcatcgcgac cgggatgccc gttttggcgg
     2461 cgaactcccg cagctcttcg gctgcgcctg aggccaccac tccgccgccg caatagatga
     2521 ccggtctcga gcttgcacga atcgcctcaa taacctcgtc gatcttccgc cccgacgggg
     2581 caggcggcag ccgatagccg ggaagatcca tggcgacgtc gtatgccggg ttggctacga
     2641 ccgtgttttg aacgtccttg ggcatatcga tcaacaccgg accgtggcgg cccgtactcg
     2701 cgaggtgaaa cgcctcccgg acaatcctgg cgatgtcttt cgcgtcctgt accaggtaat
     2761 ggtgcttggt gatcgcgcgc gagacctcga ccatcggtgt ctcttgaaac gcatcggtgc
     2821 cgatgacgtg c
//
LOCUS       DATAQL010000447         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2945814, whole genome shotgun sequence.
ACCESSION   DATAQL010000447 DATAQL010000000
VERSION     DATAQL010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945814"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            887..>4610
                     /locus_tag="VHS97_07725"
     CDS             887..>4610
                     /locus_tag="VHS97_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX3448129.1"
                     /translation="MTLHTLARLLFAGILLSSFRSAEAADAPAQPVDVDYTAKIREHT
                     TEPFFLTELVDHLPSSDAVPSPQKALGYVVGTPEKLTYTKDINAYFRKLAEASPRVKV
                     WTIGKSEEGREMLLAALSSEENLARIDRYKQITATLADPRKTTDAESADLVREGKPFY
                     WLSGSIHSPETGSPEMLMELAYRLAVEESAHVQAIRKNLVVLITPVVEVDGRDRMLDV
                     YRYRKANPGKQAPGLVYWGKYVAHDNNRDAMGLALALSNVMMKTFLAWHPQVFHDLHE
                     SVPYLYTSTGTGPYNSWLDPIVVSEWQKLAYHEIEGMTRRGVPGVWTHGFYDGWAPNY
                     MFYIANGHNAIGRFYETFGNGGADTRERKLPDSATSRTWYRPNPPLPRIKWSLRNNVN
                     MQQSALLLVLKYVADNREEFLNNFALKSKRSVSKARTEGPAAWIIPNDGKRPALAAQL
                     ARLLQRQGAEVHRLDREAEVKVAKPSPARGRPTAQPGGDPAPEPKAEATKPQAQKVAA
                     SSFVVRMDQPYCRMVDMMLDTQYYSTADPRPYDDTGWTFGPLRNVVTLRVVDPSILDV
                     PMTKIDGEVRANGGVEGNGYAWFILNANAEPALASLRFRLKDVKMFAAEEPFEVEGGK
                     YSAGSFLIPTSGNPEDLLSQLRSATESLGLKAHAISSEIKVKRHPVSVPRIALLHTWV
                     NTQNDGWFRLALDECEVPYAYISDQDIRATPDLKAKYDVIIFPPVSSSLPTLINGVRK
                     RLLDDGSEFGGPVPFKSTELTPNLGGVDDSDDIRGGLGFDGLAHLKTFVENGGVFMPI
                     TASANLPVGLGMVEHVTIAETRQLQANGSVLRAGVQDKRSPIAYGYDDTVALYFNQAP
                     VFRLSLTGGGFGGRGGGAGGPSGESAGRPSGRGSATDPDIPQGRPLREFEREPTMGPA
                     ERELHIEPEMREFLAGTILPPKMWPRVVVRWSDEKDLWVSGMLAGGSELAGTPAVIDV
                     PLGKGHVVLFGNNPMWRHETHGSFMLLLNTALHYDHLHAGRNEPASDKRVVRQYTIQQ
                     FLATTAVSGAAFSSDGSRVLFTSDASGIPNAYTVPFEGGKVTPLTRSTADSTFAVSFF
                     PKDERILYTHDRGGDENNHLYVLGPKGEIDLTPGTKLKAMFAGWSRDESSFNALTNER
                     DPRFFDVYRYDADKLDRTSIYKDTVGYEVAAVSGDAQWVALGKPTSTADADIYVWDVL
                     GARMTHLTPHKAPTQFHASGFDHDSKSLYYITNSGGE"
BASE COUNT          928 a         1513 c         1350 g          819 t
ORIGIN      
        1 ggtccatcgc atgcagcggc cttccgtccg ccagcccttg gagaaccgtc aacgcgtcca
       61 agccatcgat caacagcaac gccactcggc cttcggccct gtgggtccat ttgccgtttt
      121 catgaatccc gatacggaaa ttcacttgga aatcctccat cggcgtgatc caagaacttc
      181 ttccgacaat aaccttctgt cgcgggaaac gcaaagccca ctcgacgcgc gctccagagc
      241 ttcggatccg gtttctcttt tcctaccgat gcgtgtcact ggccggaaat acggccgact
      301 gccagcgacg cccgacgccg ctgcaccaaa ctcgcggccg atgcaagtta ctggctccct
      361 ctcactcggg agagggctgg cgtgaggggc gagcgccacc gtgagcctgc tcaagttgaa
      421 ccgacggatg cgcatccttt tcccaaggcg tggcgtacga ggtacgtcgc cgccctcgcc
      481 tcggtatcgg agagggaagg gcgagcggta ttttgggccc cagcaccaga gctgggccgt
      541 gtggtccatc ctacgttttg caattcgtga cccttatgcg ggatgccagg ctcgccaaag
      601 gcgcgggtat tcgatttcta cgggataacc gtctaatcag agaaaaaaag atgccgtcac
      661 tggcagtacg ccagccaaac ataggtcatc ctgtgacttt cgaacggacc tcacttgaat
      721 gaggtcacct gttcgattca actactcgtt cggatcgacc ggctaagaca accggagggc
      781 cggagaagag ttattcgcgg aatccgggca cgaggtcgag agactggcgc gaaggccggt
      841 gattgcattc gcggcagtcg cctgtccgta cgaggggacc acaccgatga cgcttcacac
      901 gctcgcgcga ttgctgttcg ccggaattct gctttccagc tttcgcagcg ccgaggccgc
      961 cgatgcgccg gcacagccgg tcgacgtcga ctacaccgcg aagatccgcg agcacaccac
     1021 ggagccgttt tttctcactg agctcgttga ccacctacca tcctcagacg ccgtcccctc
     1081 gccgcagaaa gcgctgggct atgttgtcgg cacgcccgag aaactcacat acaccaagga
     1141 catcaacgcc tacttccgca agctggccga ggcgtccccg cgggtgaaag tctggacaat
     1201 cggcaagagc gaagagggcc gcgaaatgct ccttgcggcc ctttccagcg aggagaacct
     1261 cgcgcggatc gaccgataca aacagatcac cgccacgctc gccgaccccc gcaagacgac
     1321 ggacgccgag tcagccgacc tggtgcgcga gggcaagccg ttttactggc tctccggctc
     1381 gatccactcg ccagaaaccg gctcgcccga gatgctcatg gagctagcct accggctcgc
     1441 cgtcgaagag tcggcacatg tgcaggcgat acgcaagaac ctggtcgtcc tgatcacccc
     1501 cgttgtggag gtcgacggcc gagaccgcat gctggatgtt tatcgctaca ggaaggcgaa
     1561 cccgggcaag caagctccgg ggctcgtata ctggggcaaa tacgtggccc atgacaacaa
     1621 ccgcgacgcg atgggcctgg cattggcgct gtcgaatgtg atgatgaaga cctttcttgc
     1681 atggcacccg caggtcttcc acgacttgca cgagtcagtg ccctatctct acacctcgac
     1741 cggcaccggc ccgtataact cctggctcga ccccatcgtg gtcagcgaat ggcaaaagct
     1801 cgcataccac gagatcgagg ggatgaccag gcgcggagtg ccgggcgtct ggacccacgg
     1861 tttttacgac ggctgggccc cgaactacat gttctacatc gccaacggtc acaacgcgat
     1921 cggtcgtttc tacgagactt tcggcaacgg aggcgccgac acacgcgagc gcaaactccc
     1981 cgactctgcg acctcgcgca cctggtaccg tcccaacccg cccttaccac ggatcaagtg
     2041 gtcattgcgc aataacgtca acatgcaaca gagcgcgcta ctactcgtgc tcaaatatgt
     2101 ggcggacaat cgcgaggagt tcttgaacaa tttcgccttg aagtcgaagc gctccgtctc
     2161 caaggcaagg accgagggcc ctgctgcctg gatcattccc aacgatggca agcgccccgc
     2221 cctggccgcg cagctcgcgc gcctcttaca gcgccaggga gcggaggtcc accgtctcga
     2281 tcgcgaggcg gaagtcaagg tggcgaaacc atcgcccgcg cgcggacggc cgaccgcaca
     2341 acccggcggc gatcccgccc ccgagccgaa agccgaagcg accaagccgc aggcgcaaaa
     2401 agtcgccgcg agcagcttcg tggtgcggat ggatcagccc tactgccgca tggtcgacat
     2461 gatgctcgac acccagtatt attcgaccgc cgatccgcgt ccctacgacg ataccggctg
     2521 gacgttcggc cctctgcgca acgttgtcac cttgcgcgtt gtcgatcctt cgatcctcga
     2581 tgtgccgatg acgaagatcg acggtgaagt ccgcgcgaac ggaggcgtcg agggaaacgg
     2641 ctatgcgtgg ttcatcttga acgccaacgc cgagccggcg ctcgcgtcac tccgctttcg
     2701 gctgaaggac gtcaagatgt tcgcggccga ggaaccgttc gaggttgagg ggggcaaata
     2761 tagcgccggc tccttcttga taccaacctc aggaaacccc gaagacctcc tctcgcaact
     2821 caggtcggcc accgagtcgc tcgggctcaa ggcgcacgcc attagcagcg agatcaaggt
     2881 gaagcgccac cctgtctcag taccgcggat tgccttgctc cacacgtggg tcaacacgca
     2941 gaacgacggt tggtttcgcc tggccctgga cgaatgcgaa gtgccctacg cgtacatctc
     3001 cgaccaggat attcgcgcga cgcccgacct gaaggcgaaa tatgacgtga tcatctttcc
     3061 ccctgtttcg tcgagtctgc cgaccctgat caacggcgtc cgcaagcgtc tgctcgacga
     3121 cggctcggaa tttggcggcc cggttccatt caagtccaca gagctgacgc cgaacctcgg
     3181 cggagtagac gactccgacg atatccgcgg cggtctggga ttcgacgggc tggcccattt
     3241 gaagacgttc gttgagaacg gcggcgtgtt catgccaatc acggcaagcg ccaacctgcc
     3301 cgtcgggctc ggcatggtcg agcacgtcac gatcgccgag acgcgccagc tacaggccaa
     3361 tgggtctgtc ctccgcgcgg gggtgcaaga caaacgcagc ccgatcgcct acggctacga
     3421 cgacacagtc gcactctact tcaaccaggc cccggtcttt cgactctcgc tgactggggg
     3481 cggctttggt ggccgtggcg gaggagccgg aggacccagt ggtgaaagcg ctgggcggcc
     3541 gtcgggccgc gggtcggcga ccgatccaga cattccccaa ggccgcccac tccgtgagtt
     3601 cgagcgcgag ccgaccatgg ggcctgctga gcgcgagctc catatcgagc cggagatgcg
     3661 cgaattcctc gccggcacga tcctaccccc caagatgtgg ccgcgcgtgg tcgtccgctg
     3721 gtccgacgaa aaggacctct gggtcagcgg catgctcgcg ggtggttccg agctcgcggg
     3781 gacgccggcg gtcatcgacg tgccgctcgg gaaggggcac gtcgtcctct tcggcaacaa
     3841 tccgatgtgg aggcacgaga ctcacggcag ctttatgctc ctgctcaaca cagcgctcca
     3901 ttacgaccac ctgcacgccg gccggaacga accggcatcc gacaagcgtg tggtccggca
     3961 atacaccatt cagcagtttc tggcgacgac ggcagtcagc ggcgcggcgt tttcgtccga
     4021 cggctcgcgc gttctcttca cgtccgatgc ctcgggaatc cccaacgcct acaccgtccc
     4081 cttcgagggt ggcaaggtga cgccgctgac ccgatcgacc gccgactcga cctttgcggt
     4141 ctcgttcttc cccaaggacg agcgaatcct ttacacacac gaccgcggtg gggatgagaa
     4201 caatcacctc tacgtgcttg gcccgaaggg tgaaatcgac ctgactccag gcacgaagct
     4261 gaaggccatg tttgccggct ggtcgcgcga cgaatcttca ttcaacgccc tgaccaacga
     4321 gcgtgacccg cggttcttcg acgtctaccg ctacgatgcc gacaagctcg accgaacatc
     4381 gatctacaaa gacacggtcg gctacgaggt cgccgccgtt tcgggtgatg ctcaatgggt
     4441 ggcgctcggt aagccgacat cgactgccga cgccgacatc tatgtgtggg atgtactggg
     4501 ggcccggatg acccacctga cgccccacaa ggcccccact caattccacg catccgggtt
     4561 cgaccacgac tcgaaatcgc tttattacat caccaactcc gggggcgagt
//
LOCUS       DATAQL010000448         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4167463, whole genome shotgun sequence.
ACCESSION   DATAQL010000448 DATAQL010000000
VERSION     DATAQL010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4167463"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1323
                     /locus_tag="VHS97_07730"
     CDS             376..1323
                     /locus_tag="VHS97_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3448130.1"
                     /translation="MDEQRPVFVGIDVSKDRLDIHLRPSNEAFHVSRDGKGLDDLGSR
                     LKKLPVALVVLEATGGFEATVAAALAGIGLPLCVVNPRQIRDFARAMGRLAKTDTLDA
                     EVIALFAERIRPEVRPLPEPERNHLAELVGRRRQIIEMIGMETNRGRQTVDKHLARRL
                     ACHIAFLQKELDAIDHDIGKAIKHSPAWREAEALLKSVPGIGDVTARTLLAELPELGT
                     VSRHQVAALVGVAPINRDSGLMRGRRAIAGGRTSVRAVLYMAALTAIRRRSPFRAFYD
                     QLTARGRPKKVALVAVMRKLLTILNAILRDRTPWRHLNA"
BASE COUNT          280 a          482 c          453 g          301 t
ORIGIN      
        1 caggcggcga cggctgattt cgccgagcgg cggacgggaa ccggatttct ggctggattt
       61 gtccttgctc atcgcttcct ccttgtatgc gccgtcctgt tgcgacccag ggccgtgctt
      121 cggcggccga catgctgatc gaattgaatc gtccgtggac caggcgctgg tggtggttgg
      181 gattcagcga cgaccgggcc gccttgtatg tctgagttgc ctgcaggagt gagaccagcg
      241 aggctgaaag tggaggccag cccgcgtccg cctggggccc gaaagcccga tacagagctt
      301 ggtgcggtct ccatcatcgc agtcccacac ccggacagcc gctcgggccc gagcccgcat
      361 ttgcaaggta gggtcatgga cgaacaacgc cccgtcttcg tcggtatcga cgtttccaag
      421 gatcgcctcg acatccatct cagaccgtct aacgaggcat tccatgtctc gcgggatggc
      481 aaggggttgg acgaccttgg cagtcgccta aagaaactgc ctgtcgccct cgtcgttctc
      541 gaagcgaccg gcggcttcga agctactgtc gcggcagccc tggccggcat tgggctgccg
      601 ctctgtgtcg tcaacccgcg ccagatccga gacttcgcca gagccatggg ccgcttggct
      661 aagaccgaca cgctcgatgc ggaggtgatt gcccttttcg ccgagcgcat tcggcctgaa
      721 gtacgacctc ttccggaacc tgagaggaac cacctcgccg aactcgttgg ccggcggcgc
      781 cagatcatcg agatgatcgg catggaaacc aatcgtggcc gccaaaccgt cgacaagcat
      841 ctcgcccgaa gactcgcatg ccatattgct tttcttcaga aggagctcga cgcgatcgac
      901 catgacatcg gcaaagccat caaacattcc ccagcttggc gcgaggctga ggcacttctg
      961 aaatccgtcc ctggcatcgg cgacgtgacc gcacggaccc ttctcgccga actgcccgaa
     1021 ctcgggactg tcagccgcca tcaagttgct gccctggtcg gcgtcgctcc gatcaaccga
     1081 gactccgggt tgatgcgtgg ccgtcgggca attgctggcg gcaggacgtc agtgcgcgcc
     1141 gtcctgtaca tggcggcttt gaccgccatt cggcgtcgct ccccgttccg ggctttctac
     1201 gatcagctca ctgctcgtgg ccggccaaag aaggtcgcgc tcgttgccgt gatgcggaag
     1261 ctcctcacga tcctcaacgc catactaagg gaccgcacgc catggcgcca tctaaacgct
     1321 tgacgccgaa cacagccgct ctgtcgtggg actgtagtcc cgttggccga ccgttccagc
     1381 ataaaaattg ttggctgaca gcgagtgcac ggtcattgaa actgctgcgg ccgattcact
     1441 ggttcgatga ctatgacaac cccgctcttg agccgtacat gtcgttcggc aagtgaaggg
     1501 caaatggcag gatccg
//
LOCUS       DATAQL010000449         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1877534, whole genome shotgun sequence.
ACCESSION   DATAQL010000449 DATAQL010000000
VERSION     DATAQL010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877534"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VHS97_07735"
     CDS             complement(<1..1113)
                     /locus_tag="VHS97_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3448131.1"
                     /translation="MNDAWLADRMKHIDASGIRKAFDMSKAMKDPINLSIGLPHFDVP
                     EPVKAAAIEAIQGGHNAYTVTQGIPELRTKIQGAVDAQFGHADRQVLVTAGTSGALLL
                     ALCCVVNPGDEVIVFDPYFVMYRHLVTLAGGVSVLVDTYPDFRISKARVEAAMTERTK
                     CVVVNTPANPTGVVATAAEMQALAELCRRRDVLLISDEVYRAFCYGCPFDTPTSWNDD
                     VLVVDGFSKAYAMTGWRLGYAHGPNRLIQEMAKLQQFSYVCAPSMAQYGGVAALDQDL
                     TAQIDAYRRKRDMVVEALSDDFELSLPEGAFYAFPRAPWGTGSEFVAEAIKNNLLIIP
                     GNVFSTHDTHFRISYAVDDATLRLGLDLLRRLARSRP"
     gene            complement(1197..1727)
                     /locus_tag="VHS97_07740"
     CDS             complement(1197..1727)
                     /locus_tag="VHS97_07740"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX3448132.1"
                     /translation="MPGPATTDLRKFVRDIPDFPKPGILFKDITPLLSDPEAFRAAIA
                     RLKDHFRGRGIDVIAAAEARGFIFGAPLALEMNAGFVPIRKPGKLPYATIALEYQLEY
                     GTDKLEVHTDAFGPGHRVLLIDDVLATGGTMRACSDLVQSTGADLVACAFLIELSFLG
                     GRARLEPIEVFSLITY"
     gene            complement(2318..3685)
                     /locus_tag="VHS97_07745"
     CDS             complement(2318..3685)
                     /locus_tag="VHS97_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448133.1"
                     /translation="MSDTSILARRPTRRQFVKRLAGTAVGAIAAPAIVRGRNLNDKLN
                     IAMIATGGRGGFNLKQAAIASENIVVLCDVHEPAVNKAAQMYPQARRYSDFRRVYDRA
                     NDFDAVVVSTTEHTHAFATLPALQLGKHVYCEKPLTYNIWEARVIREAAAKTKVATQM
                     GNQNHSNDNYRRVIELIQSGAIGPVREAHVWVSRAWGRQPLEAAKKNESYGVLASEKP
                     TEIDSVPTGLDWELWLGPAPARPYNDVYWPGPKWYRWWDFGNGTMSDLGSHMNDLPFW
                     ALKLRAPRTVEAFGPPPHREIAPASMHVTYEYGPRGDLPPVKLTWYQGEDKPDLYRDG
                     TIPSTFKDGILFVGSNGMLLASHGKHVLLPEDRFRDFKRPEPTIPNSPGHWAEWVRAA
                     KTGSPTGSNFEYAGWLTEANHLGNVAYRVGRKIEWDPETLCAPNAAEAQPFIRREYRK
                     GWTLA"
BASE COUNT          730 a         1238 c         1318 g          758 t
ORIGIN      
        1 cgggcgggac cgcgccaacc tgcgaagtaa atcgagcccc aggcgcaggg tcgcgtcgtc
       61 gacggcgtac gagatgcgaa agtgcgtgtc gtgcgtagag aacacgtttc cggggatgat
      121 caagagatta ttcttgatcg cctcggcgac aaattcgctg cccgtacccc acggagcgcg
      181 ggggaaggcg tagaaggcac cttcggggag tgagagctcg aagtcatcgc tcagagcctc
      241 gactaccatg tcgcgcttgc ggcgataggc gtcgatctgg gcggtcaaat cctgatcaag
      301 cgcggccact ccgccgtact gtgccatact cggtgcacac acatagctga attgttggag
      361 cttggccatc tcttgaatca ggcggttggg gccatgggcg tatccgagcc gccagccggt
      421 catggcgtag gctttgctga aaccatcgac cacgagcacg tcgtcgttcc agctcgtcgg
      481 cgtgtcgaac gggcaaccat agcaaaaggc ccggtagacc tcgtcactaa tcaagaggac
      541 gtcgcgacgg cggcagagct cggcaagagc ctgcatctcg gcggcggtcg ccacgacgcc
      601 agtcgggttg gccggggtgt tgaccacgac acatttggtg cgctcggtca tggccgcctc
      661 gacccgcgct ttacttatgc ggaaatcagg atatgtgtcg accagcaccg acacgccccc
      721 cgccaatgtg accagatggc ggtacatcac gaagtaaggg tcaaagacga taacttcgtc
      781 tcccgggttg accacacagc acaaagccag gagcagggcc ccggatgtcc ccgcggtgac
      841 caacacctgg cggtcggcgt gcccaaactg ggcatcgacg gcgccctgaa tcttggtgcg
      901 caactcggga attccctgag ttacggtata ggcgttgtgt ccgccctgga tggcctcgat
      961 ggccgcagcc ttgacgggtt cgggaacgtc gaagtgaggg agcccgatcg agagattgat
     1021 cgggtctttc atggctttgg acatgtcgaa cgccttgcgg attcccgagg cgtcgatgtg
     1081 cttcatcctg tcggcgagcc aggcgtcatt cactaatctt agctcttgcg aggggggctg
     1141 cgggttcttc gtcgccggcg gaggcccgtt tttcacttgc gaccggctac gagtggctag
     1201 tatgtgatca ggctgaagac ttcgatcggc tcgagccggg cacggccacc cagaaaagac
     1261 aactcaataa ggaacgcgca cgcgaccagg tctgcgcccg ttgactggac caggtcgcta
     1321 caagcgcgca tcgtgccccc ggtggccaga acgtcgtcga tcaggagaac acggtgtccc
     1381 gggccgaagg cgtcggtatg tacctcgagt ttgtccgtac cgtattccag ttgatattcg
     1441 agcgcgatgg tagcgtatgg aagctttcct ggcttgcgga tcggcacgaa tccggcgttc
     1501 atctccaagg ccagcggggc gccgaaaatg aatccgcggg cctcggccgc ggcgatcacg
     1561 tcgattcctc gaccccggaa atgatccttg agtctggcga tcgctgctcg aaacgcctca
     1621 ggatcgctca agagcggggt gatatccttg aagaggatgc ctggcttggg aaaatcgggg
     1681 atgtcgcgaa cgaatttgcg gaggtcagtc gtcgccggac ctggcatcaa tctattgctc
     1741 ccatcaagct ggcggaccgg cagcgcgaac cattcaacga agagcggccc ggtacctacc
     1801 ggtcgctgcc gcaattgaac atcgcgtgct cgaaaccaat atcgggaaag cttgtcccgt
     1861 tgtccagagc acccaagcta ccgttgccaa gtgcggtcgc cacctgatga cgcgtccttg
     1921 tccgacgact gcagcggcga tcgacacgct cgttcgtgaa agggcaaggg gcgcggtgaa
     1981 atctcccact gcccgatacc tacttttccg tctcgttcga gttcccgaat ttcgttaaga
     2041 aacctcgaac tcggtgcggg ctcgcgcacc gccgctcacc agggaaggta gcaggcgatg
     2101 ttacaaactg cgcggatgga tctgcttcgt tgcgcgaact ccaaaatctc gtcaggcgag
     2161 cgttgtcggt caacctggcg ggttgccccg cccgatgaca cgatcctgac ttatcgatcg
     2221 catcgaacag tcgccggccc gttgatgagt attgcctcgt tgcgccctga aggcacgacg
     2281 aggcttccga cgggaacacg cgcaccggac ccagggctca ggccagcgtc cagcccttgc
     2341 ggtactcgcg gcggatgaac ggctgagcct cggcggcgtt gggggcgcag agcgtctcgg
     2401 ggtcccactc gattttccgg ccgacgcggt aggcgacatt gcccagatgg ttcgcctcgg
     2461 tcagccagcc ggcgtactcg aagttcgagc cggtcggcga gccggtcttg gcggcgcgga
     2521 cccactcggc ccagtggccg ggcgagttcg ggatcgtcgg ctcgggacgc ttgaagtcgc
     2581 ggaaccggtc ctcgggcagc agcacgtgct tcccgtggct ggccagcagc atcccattgg
     2641 aaccgacgaa caggatgccg tccttgaagg tgcttgggat cgtgccgtcc cgatacaggt
     2701 cgggcttgtc ctcgccctgg taccaggtga gcttcaccgg cggcaggtca ccacgagggc
     2761 cgtactcgta ggtcacgtgc atcgaagccg gggcgatctc ccggtggggc ggtggaccga
     2821 acgcctcgac ggtccgcggc gcccggagct tcaacgccca gaacggcagg tcgttcatgt
     2881 ggctcccaag gtcactcatc gtgccgttgc cgaagtccca ccaccgatac catttcggcc
     2941 cgggccagta gacgtcgttg tacggccgcg ccggcgccgg gccgagccac agctcccagt
     3001 cgagcccggt cgggacagag tcgatctcgg tcggcttctc actcgccagg acgccgtaac
     3061 tttcgttctt cttggccgcc tccagcggct gccggcccca ggcgcgggag acccacacgt
     3121 gcgcctcgcg gaccgggccg atggcgccgg actggatcag ctcgatgacg cgacggtagt
     3181 tgtcgttgga gtgattctgg ttgcccatct gggtggcgac cttggtcttg gcggcggcct
     3241 cgcggatgac gcgcgcctcc cagatgttgt aggtcagcgg tttctcgcag taaacgtgct
     3301 tgcctagctg caaggccggc agcgtggcga aggcgtgtgt atgctcggtc gtgctgacca
     3361 ccacggcgtc gaagtcgttg gcgcggtcgt aaacacggcg gaagtcgctg tagcgccggg
     3421 cctgggggta catctgcgcg gccttgttga cggccggctc gtggacgtca cacaggacca
     3481 cgatgttctc cgaagcgatt gccgcctgct tcagattgaa gccgccgcgg ccgccggtgg
     3541 cgatcatggc gatgttgagc ttgtcgttga ggttccggcc gcgcacgatc gccggcgcgg
     3601 cgatagcccc gacggcggtg cccgccaagc gcttcacgaa ctgacggcgc gtcggccgcc
     3661 gggccaggat ggaggtgtcg ctcatggagt gttctcccgg ttgatcctga ggagaggtcg
     3721 ccgcgacggt ggccacaccg tctccggtcc tgagtccatg atcggccatc cggacggccc
     3781 ttgcaatccc cccggatcga catccagggt cgaccagttc gtcggtgccg cacggtcggc
     3841 atcaacaagc cgacgctcta cgcttatgtc gaggaagtaa ggctcgccga tggcatgcag
     3901 taggatcaga acaacttaat gccgtcaagc ggtctgccga tgatgacgcg atcgcgcgga
     3961 attcggaagg tgagggccaa cgcggcggct tggactagtg gtccgtcaaa tttcgattga
     4021 cgcgtatcca agggccgagc ccct
//
LOCUS       DATAQL010000450         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_349774, whole genome shotgun sequence.
ACCESSION   DATAQL010000450 DATAQL010000000
VERSION     DATAQL010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349774"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VHS97_07750"
     CDS             complement(<1..1182)
                     /locus_tag="VHS97_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3448134.1"
                     /translation="MHKSITAFAAGLVTAVTVLSTAYADNDNHGGGSATATPIKHLVV
                     IFQENVSFDHYFGTYPHATNPTGEPRFEARPGTPTVNGLSGTLISNNPNATNPVNGAG
                     ATNPFRLDRSQAATMDQDHDYTPEQLAFDHGLMDAFPASVGTAGPPPTGAPIFATTGL
                     TMGYFDGNTVTALWNYAQHFAMSDNSYDTNFGPSTPGAINLISGQTNGLTSNMAPGGA
                     TIPDGGGGLSLISDADPLGDTCSTTTGELVHFNGKNVGDLLNAAGVSWGFFEGGFDLS
                     VVNADGSTGCKRSTTSKITNTKKADYIPHHQPFQYYTSTANPTHARPKSVHSIGQTDA
                     ANHQYDTHDFFDAVNAGNFPAVVFLKAPGFQDGHAGYSDPLDEQTFIVNTINFLQKQR
                     DW"
     gene            1465..>1929
                     /locus_tag="VHS97_07755"
     CDS             1465..>1929
                     /locus_tag="VHS97_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM-like peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HEX3448135.1"
                     /translation="MAAAAFMGWRLGRTPEPIAAAPSSADAAQASATIAAPATPPPIA
                     VQQASVASIVEVVVGRNDTLDAIFRRMALNKSDLAAIRNLPGIRQSLDFLKPGDSIKL
                     THTDGDIKELTRKVSETQTLDVVLKDSGFEAKMIDNPVETRVRTARATIDSSL"
BASE COUNT          386 a          560 c          604 g          379 t
ORIGIN      
        1 ccaatcgcgc tgcttctgca ggaaattgat ggtgttgacg atgaaggtct gttcgtccag
       61 tggatccgaa tagcccgcat gaccgtcctg aaagcccggc gccttcaaga aaaccaccgc
      121 cgggaaattg cccgcgttga ccgcgtcgaa gaaatcatgc gtgtcgtact ggtgattggc
      181 ggcatcggtc tgcccaatcg agtgcaccga tttcggccgc gcgtgcgtgg ggttggcggt
      241 cgaggtgtag tactggaaag gctggtgatg cgggatgtaa tcagccttct tggtgttggt
      301 gattttcgaa gtggtgctgc gcttgcaacc cgtcgagccg tccgcgttga ccacggacag
      361 atcgaagccg ccctcgaaga atccccagct cacgccggcg gcgttcaaca agtcgccgac
      421 attcttgccg ttgaagtgca ccagctcgcc ggtggtcgtg gagcaggtat cgcccagcgg
      481 atcggcatcg ctgatcaggc tgagaccgcc gccgccgtcc ggaatcgtgg cgccgcccgg
      541 ggccatgttg ctggtgagcc cattggtctg acctgagatc agattgatgg cgcccggcgt
      601 cgagggaccg aagttcgtgt catacgagtt atcgctcatc gcgaagtgct gcgcgtagtt
      661 ccaaagcgcg gtgacggtat tgccgtcgaa ataacccatc gtgagacccg tggtggcgaa
      721 gatgggcgcg ccggtcggcg gcggacctgc cgttcccacc gaggcgggga aggcatccat
      781 caagccgtga tcgaatgcca gctgctcggg agtgtagtca tggtcttgat ccatcgtcgc
      841 cgcctggctg cggtcgagac ggaaagggtt ggtcgccccg gccccattga caggattcgt
      901 ggcattcgga ttgttggaga tcaaggtgcc cgacaatcca ttcaccgtcg gcgttccggg
      961 cctggcctcg aaacgcggct cccccgtggg gttcgtcgca tgcggatagg tgccgaaata
     1021 gtgatcaaac gacacgtttt cctggaatat gacgacaagg tgcttgatgg gagttgccgt
     1081 ggcgcttccc ccaccatgat tgtcgttgtc cgcgtatgcg gtgctgagga cggttaccgc
     1141 cgtaaccagt cccgcggcga acgccgtgat ggatttgtgc atcgtttata cccctcaaat
     1201 gatggaaaaa taaaacttgc gactttttag ccagacaaga tgaacggctc attacagcgg
     1261 ggaagaattg gaggcacgca ccaagccgtg gtcgtagcgg tcgacacaaa cgacccgagg
     1321 ccgaagattg accccatggg gagcgacgga tagcctacgg agttatctcg ctaggaattg
     1381 ctggtgccac gcaacatatt cggaccctgg aaaaattctt cgcgaatcaa gatattagcg
     1441 acttgcgcgg tggtttgcgg cgccttggcg gccgcggcat tcatgggatg gcgcctcggg
     1501 cgaactcctg agcctatcgc tgctgccccg tcctccgccg acgccgccca ggcatccgcg
     1561 acaatcgccg ctccggcgac accgccgccc atcgcggtgc aacaggccag cgtcgcgtcg
     1621 atcgtggaag tcgtggtcgg cagaaacgat acgctcgacg ccatttttcg gcgcatggcg
     1681 ctcaacaaat ccgacctcgc ggcaattcgc aacctgccgg gcatccgtca gagcctggac
     1741 ttcttgaagc cgggcgattc gatcaagctc acgcacaccg acggagacat caaggaactc
     1801 acccgcaagg tcagtgagac gcaaactctg gacgtcgtac taaaggacag cggcttcgag
     1861 gcgaagatga tcgacaatcc ggtggagacc cgggtgcgca ccgccagggc caccatcgat
     1921 tcatcgctg
//
LOCUS       DATAQL010000451        12044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4472702, whole genome shotgun sequence.
ACCESSION   DATAQL010000451 DATAQL010000000
VERSION     DATAQL010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4472702"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1870
                     /locus_tag="VHS97_07760"
     CDS             485..1870
                     /locus_tag="VHS97_07760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3448136.1"
                     /translation="MRITFHGAARQITGSAHLLEIDSRRILLDCGLFDSDRIDPNSPN
                     RQFPFDPRSLDAVIVSHAHNDHIGRLPCLIRAGYAGPIFATPATADITNVMLRDSARI
                     QREEVRNAHLKYPHIEAPEPLFDLADVEWMVERLKRVAYQEPLEVVPGVTLTYLDAGH
                     ILGSAVVQLDFRENGRPRRFVFTGDLGRRNTHLLPDPTPVKNIDILVSESTYGNRELD
                     PYDRLMKQLHAIIARATRLQSKIVIPAFSLGRTQRMVYCLQELFAVHKVRPIPIYVDS
                     PLAMRLTEIHRDHPSAYTPLARALMDKDPLYFGSKYVEFCASWDDSRRLNHMNGPMVI
                     VASAGMCEAGRIRHHLRHVVANPDNAIVIVSYQAEGTLGRRISEGVERVQIFDQWYDL
                     NAAVYVLDGFSGHGDRGDLGWWYEQTGGQIENAFLVHGEPESMEALAPLLQPFSQSPV
                     HTPEVNTSYEV"
     gene            complement(1874..2305)
                     /locus_tag="VHS97_07765"
     CDS             complement(1874..2305)
                     /locus_tag="VHS97_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX3448137.1"
                     /translation="MSPSSNEPSTDCNNTMPPAEDRRLVRLTKICLALPEASRVLSGD
                     HAGFTVRKKTFGYFLNNHHGDGIVAVTFKAAPGDNAKLVALDPTRFYLPAYVGPRGWV
                     GLRLDVGKVDWDEVADFVGDSYRLVAPARLVALANQRASES"
     gene            complement(2302..2937)
                     /locus_tag="VHS97_07770"
     CDS             complement(2302..2937)
                     /locus_tag="VHS97_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448138.1"
                     /translation="MTKVLRSWTVLPVWIIVMLSAGGCNEADSEDQPNLPPPGTGGGM
                     PGGGGPTGGSSSPGIRQIMVKLAKGPNSLTPVIGNELQQDSPPWDTIQGQAKEYAQSA
                     AELGKYDPPKGAKESWAKLTAAFAESGAELERAAMAKDKEAARLSHDQLKNSCNACHQ
                     EHRRMGRGGGPGGPPPGGPGGPPPGGFGRPPAGQGGPPPGGRGGPPPGTPG"
     gene            complement(3230..5425)
                     /locus_tag="VHS97_07775"
     CDS             complement(3230..5425)
                     /locus_tag="VHS97_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3448139.1"
                     /translation="MEDGQTSGLGATEVGTRSSNSSEPESATWSAQLTAYHPSAAFRR
                     ALNREAHPRRTGAESEPSSTAATDGLGHAPGILSLLGAGMAVGATAGFLELSVQAAQI
                     HGLHRVDWSSLTISRHAGWMVVVSSTLLSASLTLLILAPALAWAAWRQRRQVPVHRLS
                     WTWDLAGAILGTLLLLGPLQTIQGLHPAAPASVALGAGIRCRRWLVWRSAAWQRAACR
                     LAMLVAGTLPFYALWQWHSVTSAPERVWSQPGARTPNVLWIVADTLRADHMSCYGYNR
                     PTTPEIDKWAKQGITFEMARSAAPWTLPSHVTMFTGLWPAQHGARVDRSYYGPSPTIA
                     EHLRARGYATAGFVSNVRICNLAYGVGRGFDTFVDYPCNQEISLRAAIINCSLGASVM
                     EVANRLQLPVPRPFPFYLKPDARKITDQGRQWLGEVVGRNESVGPGATRPFFLFLNLM
                     DVHGPFIPSSSATGKFWTGPIPPVKQSSPECGWLAVRELDHASPEMRSERRHELEKVS
                     RRLIDLYDECLSGLDADLGRFLGGLRSAGMLENTWVVITGDHGEHFGEHNHFGHGSSL
                     YNELTHVPLILIPPLGSGESGCDQASSLRGRRIAVPVSLRDLPATVADLIDPNTDHPF
                     PGRSLARYWSAAGEVSADPVLSQLDDPSLRGTDFRTESVTSVDSLILENHILIHGRNQ
                     PLELYHLFNDPLQQHNLAERSSERARTARLKDTLEVLRSELGSSLEPPL"
     gene            complement(5703..5945)
                     /gene="dltC"
                     /locus_tag="VHS97_07780"
     CDS             complement(5703..5945)
                     /gene="dltC"
                     /locus_tag="VHS97_07780"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF003464.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEX3448140.1"
                     /translation="MTISDRVLGVLVDVSEIEGIRHNPDVRLYDLQILDSMQTVRLIV
                     ALSSEFGVEISPAEIDREEWATPRKIISFMERKVAA"
     gene            complement(5975..7390)
                     /gene="dltB"
                     /locus_tag="VHS97_07785"
     CDS             complement(5975..7390)
                     /gene="dltB"
                     /locus_tag="VHS97_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEX3448141.1"
                     /translation="MIPYADFLYFGILLYIAVPTLLIRRLLGFSRAWVVLVTAAMLIV
                     QYGTIAHLLPATAPEGVWFTGGSPVSGGLVKVRDLWVVIACGFFQWGVARAFLTTRTR
                     TAWYWPFPAALLLTLLPLSAARFLPLAIPGAQLGFLGISYVTFRSLDVIFGIRDRLIV
                     SLPSDQFLAFLFFFPAISSGPIDRYRRFSHDWNRPHLLAEFWNDLDGAVHRIFTGFLY
                     KFILAALIKSYWIDRLDGGGFLYNLSYLYGYSLYLYFDFAGYSAFAVGFSYLLGIHTP
                     ENFNRPFLAGNIKDFWNRWHISLSTWLRDHVYMRFMLAAAKGRWFTGKYTASYLGYFL
                     TFGLMGLWHGIEPHYLLYGLYHGTLLVGHDLFTRWNKPRRVWGNGPLWRATGTLVTFH
                     MVCLGFLLFSGRIGPTWQPATSVPPATQGAAPVHAGLHESSDDQTIKGIGPRPRVIIR
                     TSERPRRALATTRRSAALAME"
     gene            complement(7387..8844)
                     /gene="dltA"
                     /locus_tag="VHS97_07790"
     CDS             complement(7387..8844)
                     /gene="dltA"
                     /locus_tag="VHS97_07790"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEX3448142.1"
                     /translation="MELIERIDHWGRTTPDHPAYISGGRTLTYGELCRRSDVLAAHLA
                     ASLPEKAPVVVLGHKEPELLIAYLGAIKSGRAYVPVDTAVPPQRIERIRAVSGAPLIL
                     SPEKVAELSSGRAAAPPWRLGTHDHYYIMFTSGSTGEPKGVPITYGCLQSFLRWILAE
                     HAFLEGREVFLNVVPYSFDVSVMDTYPALVTGGTVTSVSKTELANPRQLYQLLAASAL
                     TTWVSTPSFAQMCLVEPTFNQGMLPLLRRFLFCGETLAPEVASQLLDRFPAVEVWNTY
                     GPTEATVATTSLRITRAVLEKYSPLPIGYPMAGSRVLVMDDQRRELPAGERGEIIISG
                     PNVSPGYLNRPELTETAFFHQNGQRAYRTGDWGRYRDDMLFFEGRIDNQVKLNGYRIE
                     PADVEANLRALAGVRDAVVLPVLRQGAVDSLAAFVILNERPARTDFQLACDLKVQLTE
                     HLPAYMIPRRFSFLETFPMNTNGKADRRRLAEVLT"
     gene            complement(8855..9031)
                     /locus_tag="VHS97_07795"
     CDS             complement(8855..9031)
                     /locus_tag="VHS97_07795"
                     /inference="COORDINATES: protein motif:HMM:NF023875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEX3448143.1"
                     /translation="MSLGDQLGKDNGPLSEWFARPWVRFVALTIYYLGILVGLVALYG
                     AGEFSTPPFIYQNF"
     gene            complement(9028..10500)
                     /gene="dltD"
                     /locus_tag="VHS97_07800"
     CDS             complement(9028..10500)
                     /gene="dltD"
                     /locus_tag="VHS97_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEX3448144.1"
                     /translation="MMHGQHQPLEQTPHLAPALTAVILGVVLMAAVGFYARSLEYRSI
                     KALAADEAIIERNGKLAPVKNQGTALQQAALDTDCLLPIYGSSELNLQAVYNRPFHAT
                     NLFHDQPTGFTVFPVGKAATTCLIILQKLAAVGPALKGRKVAVSLSPFWFFDWLTVRA
                     DGYAGNFSPLHAGELAFNTRLSLQLRQDAARRMLQYPETVANRPLLRFALENMADGSP
                     LGLACYQAVFPLGIVHNAILRNLDHWSVVYYLWKHPIRTSSPISPRSDRPLDWPTLHR
                     QADALYSAHSNNNEFGLDNEKWNRELRQEMFLERNTRSEKAFLLTLQKTQEWVDLELL
                     LRELTELGAQPLLLSMPIHGGWFDQCGVTYTARRAYYQELRATGARYHAAVVDFADHD
                     ADRSFCHDNLGHLAPRGLVYYSQILDSFFHDAIPSQPELPASAPVASRATEVGLLSPP
                     GPGSHPPSEGLLDPSSATAIEVRAPRTNPEPTFQGRKGKP"
     gene            10950..>12044
                     /locus_tag="VHS97_07805"
     CDS             10950..>12044
                     /locus_tag="VHS97_07805"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3448145.1"
                     /translation="MMRLFARRQDSRPSSEPCTGSAQFRAADRPCQRARRRARPEAEM
                     LDTRLLMASGQLGMADATASPDLADRVAQAVKPYLAQNQVPGISVAVVTDGQVALAQG
                     YGNSNVATGSPVQADTRFDIGSVTKTFTALGVLLLYQESQGTSHPLNLDAPISQYLHN
                     NKSFKLPPKWSRITTRELLDMTSGIRDVGGAQPWQAQLASIAKYPLLYAPGTESSYSS
                     ANYDLLGELIEQWTGETYGTYIQDQILGPLGMTGTQELGRSATVPNQAVGYGAPRRGK
                     WTKAAVQNGPALYAAAGMVSTAQDMATYMTALLSDHLLDPATTRLMWTGIPTPQHGPG
                     GPSTGVPGLGWDTAIDTSDGPVAIYKSGSVP"
BASE COUNT         2403 a         3779 c         3651 g         2211 t
ORIGIN      
        1 gcctggcagg tactcgcatc gaggtgcttg gctgattgag tccctagcaa atattacgcc
       61 gactcccggg cttgttcaac aacgatttct cttttcggcg cgccgtgttg ctcgcttgca
      121 acggaactgt tgagggacga tcgggccgcg cagtggattg accacggata gagggtccca
      181 acttcgggaa cggcgcgtgg cgccgcgacg taaacttatg cttggaatgg ctccgcgact
      241 ttcgctgttt ccgacttacg cttgtccctg gcttgaagca atcgaacctt cctgtcatga
      301 tggaagtatt atcagctagg ggagaaccgc aaatgcaggc ccagcctcat ccagccgaaa
      361 tacggcctgg gaggcgggtc agcttaggga cgattggccg gtgctgtgcc gatcgcgttg
      421 cccagcagaa cgcgtgccgt tgccgcagcg caggcgctcg atcgatcgtc gggaggtagc
      481 ttggttgcga atcacgttcc acggcgcggc ccgccagatc accggcagcg ctcatctttt
      541 ggagatcgat tccagacgga tcttgctcga ttgcgggttg ttcgattccg atcgcatcga
      601 tcccaacagc cccaaccgcc agttcccctt tgacccccgc tcgctcgacg cggtcatcgt
      661 gtcgcacgcc cacaatgacc acatcgggcg tctcccctgc ttgatccgcg cgggctacgc
      721 cggtccaatc ttcgcgactc ccgccaccgc cgatatcacc aacgtcatgc tccgcgacag
      781 cgcacgtatc cagcgcgagg aggttcgcaa cgcccatctc aagtaccccc acatcgaggc
      841 gcccgagccc ctatttgatc tggccgacgt cgagtggatg gtcgagcggc tcaaacgcgt
      901 cgcctatcaa gagccgctgg aagttgtgcc gggagtcacg ctgacctacc ttgacgccgg
      961 ccacatcctg ggttcggcgg tcgtccagct cgactttcgc gagaacggcc ggccccgtcg
     1021 gtttgtcttt accggcgacc tgggccgccg caacacccat ctcctcccgg atcccacacc
     1081 cgttaagaac atcgacatcc tggtctcgga aagcacctac ggcaaccggg agctcgatcc
     1141 ctacgaccga ttgatgaagc agctgcacgc cataatcgcc cgggcgaccc gtctgcaaag
     1201 caagatcgtc atcccggcgt tcagcctggg acgcacccag cgcatggtct attgccttca
     1261 ggagcttttc gccgtccaca aggtgcgccc gatccccatc tacgtcgata gcccactggc
     1321 catgcgcctg accgagattc accgcgatca tcccagcgcg tacacgccac tagcccgcgc
     1381 cttgatggac aaggacccgc tttatttcgg ctccaagtat gtcgagttct gcgcgagctg
     1441 ggacgactcg cggcgcttga accacatgaa cggcccgatg gtgattgtcg cgtccgccgg
     1501 catgtgtgag gctggccgca tccgccatca cctgcgccac gtcgtcgcca acccagacaa
     1561 tgccatcgtg atagtcagct accaggccga gggaacactt ggacggcgga tctccgaagg
     1621 tgtcgagcgc gttcaaatct tcgaccagtg gtacgacttg aacgccgcag tctacgtcct
     1681 cgacggcttc tccggccacg gcgaccgcgg cgacctcggc tggtggtacg aacagacagg
     1741 cggccaaatc gaaaacgcct tcctcgtcca cggcgagccc gagtcgatgg aggcgttggc
     1801 cccgctgctc cagccttttt cgcaaagccc cgtccacacg cccgaggtca acacaagcta
     1861 cgaagtttga gcctcaactt tccgatgccc gctggttggc aagtgcgacc aggcgcgcgg
     1921 gagcaacgag gcggtagctg tcgccaacga agtcggcgac ttcgtcccag tcgactttgc
     1981 cgacgtccaa ccgcaggccg acccatcccc tgggtccgac atacgcgggc aggtagaagc
     2041 gggtgggatc aagagcgacc agctttgcat tgtcgccggg cgctgccttg aaggtgacgg
     2101 cgacgattcc gtcgccatga tgattgttga ggaagtagcc gaacgtcttc ttccggaccg
     2161 tgaaaccggc atggtctccg ctcaacacgc gagatgcttc cgggagcgca aggcagatct
     2221 tcgtcaaccg gaccaggcgt cgatcttcgg cgggaggcat ggtgttattg cagtcagttg
     2281 atggttcgtt cgagctggga ctcatcccgg cgtacccggc ggtggcccac cccgtcctcc
     2341 cggcggtggc ccaccttgtc ctgccggcgg cctgccaaat ccgcctggcg gcggcccacc
     2401 cggtcctccc ggcggcggcc cgcccggccc tccgccgcgg cccatccggc ggtgttcctg
     2461 atggcatgcg ttgcacgaat tcttgagctg gtcgtggcta agcctggcgg cttctttgtc
     2521 cttggccatg gcggcgcgtt ccagctcggc gcctgactcc gcgaacgcgg ctgttaactt
     2581 ggcccaagat tctttcgccc ccttgggcgg gtcatacttg cccagttcgg ccgcggactg
     2641 cgcgtattcc ttcgcttgcc cttggatcgt gtcccaggga ggagagtctt gctgcagctc
     2701 attgccgatg acgggtgtca gcgagttcgg tcccttggct agtttaacca tgatttgccg
     2761 gatgcccggg gagctgctcc cgccggtcgg tccacccccc ccaggcatac cgccgccggt
     2821 tcccggcggt ggcaggtttg gttgatcttc gctgtctgcc tcattacaac cacccgcact
     2881 gagcatgaca attatccaga ccggcaacac agtccaacta cgcaacactt tcgtcatctc
     2941 aaagacctcg ggacgttaga aatcatgtca aagtaggcgg cagaaaagct cttcgacttt
     3001 cctgtgacaa tcaaacatcg cgaaatatcg ccttcaccca ggcgccgtca cgcgatgtga
     3061 tagtgcgcca cttgattatc tgacggtgcc ctggctgcgg caattcgcgt ctgactgtcg
     3121 cggcgattat atgaaccctc ggtccgtcgg accgtaaaga tttgtcaatg aagcgggttg
     3181 ccgccgatga aacgtcgaag ggctttttgc caattcgaga tcaccgcaat caaagcggcg
     3241 gttcgagcga gctgccgagt tcggagcgca gaacctccag cgtgtctttc aatcgggccg
     3301 tccgcgcgcg ctcggacgac cgttcggcaa ggttgtgttg ctgcaatgga tcattgaata
     3361 aatgatacag ctcgagaggt tgattacggc catggatcaa gatgtgattc tcaaggatca
     3421 gtgagtcgac acttgtcacg ctctcagttc gaaaatccgt cccgcgcagt gagggatcat
     3481 cgagctgcga caggaccgga tcggccgaca cctccccggc ggcactccag tagcgagcca
     3541 gactgcgccc gggaaatggg tgatcggtgt tcgggtcgat cagatcggcc accgtggccg
     3601 gcaggtcacg cagagacaca ggcacggcta tccgccggcc gcgcagggag ctggcttggt
     3661 cgcaaccgga ttcgccggag cccagcggag gaatcagaat cagcggcacg tgagtcagct
     3721 cattgtacag gctcgatcca tgtccaaaat gattgtgctc gccgaagtgc tcgccatggt
     3781 cgcccgtgat gacgacccag gtattttcga gcatcccagc ggatcgcagt ccgccgagga
     3841 accgacctaa gtcggcgtcg agtccgctca aacactcatc gtagagatcg atgagacgtc
     3901 gagagacctt ttccagctcg tgccggcgct cggatcgcat ttcgggactg gcgtgatcca
     3961 gctcacgcac cgcgagccag ccacactcgg gagaggattg tttcaccggt gggatcgggc
     4021 cagtccagaa ctttccggtc gcgctcgatg acgggataaa cgggccgtgc acgtccatca
     4081 agttgagaaa aagaaagaaa ggccgcgtcg cgcccgggcc tacgctctcg ttgcgcccta
     4141 ccacttcgcc gagccattgg cgtccttgat ccgtgatctt gcgcgcatcg ggcttcaggt
     4201 aaaaaggaaa cggtcgtggg accggcaatt gcaaccgatt ggcgacttcc atcaccgagg
     4261 cgcccagcga gcaattgatt atcgccgccc ggagagatat ctcctggtta cagggataat
     4321 cgacaaaggt atcaaacccg cgccccacgc cataagcgag gttgcatatc ctcacatttg
     4381 aaacaaagcc ggccgtggca tagcccctgg cgcgcagatg ctcagcgatg gtgggcgacg
     4441 gaccgtaata agaacggtcg acgcgcgcgc cgtgctgcgc cggccagagc ccggtgaaca
     4501 tcgtcacgtg cgacggcaag gtccacgggg cggccgaacg cgccatctcg aaggtgatcc
     4561 cctgtttggc ccacttgtcg atctcaggcg tagtgggccg attatatcca taacaactca
     4621 tgtgatcggc gcggagcgta tccgcgacga tccagagcac gttcggcgta cgtgcgccag
     4681 gttgagacca gacacgctcg ggcgcgctgg tgaccgagtg ccactgccac agggcataaa
     4741 acggaagagt gccggccacc aacatcgcta gccggcatgc cgcgcgctgc cacgcggcag
     4801 agcgccagac caaccagcgt cgacatcgaa taccggctcc cagcgcgacc gacgcgggcg
     4861 ccgccgggtg caacccctga atcgtttgca atgggccgag caacagaagg gtgcccagga
     4921 tcgctccggc taggtcccac gtccaggaca gtcggtggac cggcacctgc cgcctctgcc
     4981 gccaagccgc ccaggccagt gccggcgcca ggattaggag tgtcaaactc gccgacagca
     5041 gagtcgaact gacgacgacc atccagcccg catgccggct gatcgtgagg ctgctccaat
     5101 ccacacggtg caaaccatga atctgtgccg cctgaaccga caactcaaga aatccggccg
     5161 tcgcgcccac tgccatccct gcacccaaaa ggctcagtat cccaggtgca tgccccagcc
     5221 cgtctgtggc agccgtcgaa gacggctccg attcggcccc ggtgcgacgc ggatgtgctt
     5281 cgcggtttaa ggcccggcga aacgcggccg acggatggta tgccgtcaat tgggctgacc
     5341 acgtcgcgga ttccggctcg gaggagttgc tgctccgtgt tcccacctcg gtcgcgccca
     5401 agccgcttgt ctgcccgtct tccatggcaa actatcctca gagctttgag ccacggcccc
     5461 tcgtccagcc ccgctcttat cggcgatccg cgtcggcaac tcgagtgaga ttcttcaata
     5521 atcgtgtaaa agaatcttaa gtcgctagta gcaactcggg aaagagcggc gaattaatcg
     5581 acggcgcttc aagctgaggg gtcgccccat ggatggtcag aaaccttgtc cccgttcgtt
     5641 cggccttcaa accggggcga acaggagcag cacggcaggc gctcggtgga gttgtggtgc
     5701 cctcaggcgg cgaccttgcg ttccatgaag gagataatct tgcgcggtgt cgcccactcc
     5761 tcgcggtcga tttccgcagg cgagatctcg acgccaaact cggacgagag ggcgacaatc
     5821 agcctgacgg tctgcatcga atccaggatc tggaggtcgt acagtcgaac gtcgggattg
     5881 tgccgtatac cctcgatctc ggacacgtcg acaagcacgc ccaagacgcg atcagaaatc
     5941 gtcatcggaa gaatccctcc agctccgtcg tccttcattc catcgccagt gcggccgaac
     6001 gccgagtcgt tgcgagcgcc cgccggggcc gttcactcgt cctaattatt acgcgtggtc
     6061 gaggcccaat tccttttatg gtctggtcat cactcgactc gtggagaccc gcatggacag
     6121 gcgcggcccc ctgggtcgcc ggcggcacgg aggtggccgg ctgccacgtg ggaccgatcc
     6181 ttcccgagaa gaggaggaag cccaggcaga ccatgtggaa cgtgaccagg gtccccgtcg
     6241 ctcgccacag cggcccgttc ccccacaccc gacgtggttt gttccaacgg gtaaagaggt
     6301 catgccccac caacagcgtc ccgtggtaca ggccataaag caggtagtgg ggctcaattc
     6361 cgtgccacag ccccatcagc ccgaacgtga ggaagtagcc gaggtatgag gccgtgtact
     6421 tgccagtgaa ccagcggccc ttggcagcgg ccaacataaa tctcatgtac acatgatcgc
     6481 ggagccaggt ggagaggctg atgtgccagc ggttccagaa atccttgatg ttgccggcga
     6541 gaaagggccg gttgaagttc tcgggcgtgt ggatccccag caggtagctg aacccgacgg
     6601 cgaaggcgct gtaaccggcg aagtcgaagt acaggtagag actgtacccg tacaagtacg
     6661 acaggttgta gaggaatccg ccaccgtcga ggcgatcgat ccagtagctc ttgatcaagg
     6721 ccgccaggat gaacttgtac aggaagcccg tgaagatacg gtggacagcg ccatcgaggt
     6781 cgttccagaa ttccgcaagg agatgcgggc gattccagtc gtgactgaac cggcggtaac
     6841 ggtcgatggg gcccgacgaa atggccggga agaagaagag gaaggccaag aactggtcgg
     6901 aggggagcga aacgatcagt cggtctcgta tgccgaagat gacgtccagg ctgcggaacg
     6961 tcacgtacga gatccccaag aaccccagtt gggcgcccgg gattgccagc ggcaggaatc
     7021 gggccgctga cagagggagc aaggtcaata acagcgccgc ggggaagggc cagtaccagg
     7081 cggtgcgcgt cctggtcgtc aagaaagccc gggccacacc ccattggaag aagccgcacg
     7141 caatcactac ccaaagatcc cggaccttca cgagaccgcc ggagacggga gagcctcccg
     7201 tgaaccaaac tccttcggga gcggttgccg gcagtaaatg cgcgatggtc ccgtactgga
     7261 cgatcagcat cgccgcagtg acgagcacta cccacgcgcg cgagaagccg agcaaccgcc
     7321 tgatcaacaa cgtcggcacg gcgatgtata gcaggatgcc gaaataaagg aaatccgcgt
     7381 acgggatcat gtcaggacct cggccaacct gcgccggtcc gccttgccgt tggtattcat
     7441 cggaaaggtc tccaggaagg aaaaccgccg agggatcatg tacgcaggca gatgctcggt
     7501 caactggact ttcaggtcgc aggccagttg gaaatccgtg cgtgcgggcc gctcattcag
     7561 gatcacgaag gcggccaggg aatcgacggc cccttgcctg agcaccggta ggaccacggc
     7621 atcgcgaacc cccgccaggg cccgtaggtt cgcttccaca tccgccggct cgatgcggta
     7681 gccgttcagc ttgacctggt tgtcgatccg gccctcgaag aagagcatgt cgtcgcggta
     7741 gcggccccag tcgccggttc ggtaggcccg ctggccgttt tggtgaaaga acgcggtttc
     7801 ggtcaactcg ggccggttga ggtacccggg gctgacgttg gggccggaaa tgatgatctc
     7861 cccccgctca cccgccggca gctcgcgccg ctggtcgtcc atcaccagga cacggctgcc
     7921 cgccatcggg taaccgatcg gcagtggcga gtacttttcg aggaccgcgc gtgtgattcg
     7981 cagcgaggtg gttgccacgg tcgcctccgt ggggccatag gtgttccaga cctccacggc
     8041 cgggaagcgg tccaggagct gggatgcgac ttccggggcc agcgtctcac cgcagaacag
     8101 gaaccgcctg agcaagggca gcatgccctg gttaaaagtc ggttcgacca ggcacatctg
     8161 agcgaacgaa ggggtggaca cccaggtcgt caatgccgag gcagccagga gctgatacag
     8221 ttgcctcggg ttcgcgagct ccgtcttgct gacgctggtc accgtccctc ccgtcaccag
     8281 ggcggggtaa gtatccatca cggaaacgtc gaacgagtag gggacgacgt tcaaaaagac
     8341 ctcccggccc tctaggaagg cgtgctccgc caggatccac cgcaggaaac tttgcaggca
     8401 cccgtaggta atgggcaccc ctttgggctc cccggtgctc cccgaagtaa acatgatata
     8461 ataatgatca tgagttccca gacgccatgg cggagccgcg gctcgtccgc tggagagctc
     8521 ggcgaccttt tccggagaca ggatgagcgg ggcgccactg accgccctga tgcgctcgat
     8581 ccgctgcggg ggcacggcag tgtccaccgg cacatacgca cgccccgatt tgatggcgcc
     8641 cagataggcg atcagcagct ccggctcctt gtgtcccaag acgaccaccg gcgccttctc
     8701 cgggagcgac gcggccagat gggctgccag gacgtcggac ctgcggcaca attccccgta
     8761 ggtgagggta cgacccccac tgatgtaggc agggtgatcc ggggtcgtcc ttccccagtg
     8821 atcgatgcgc tcgatcaatt ccatgcgaga tcccctagaa attctggtag atgaaagggg
     8881 gtgtggagaa ctcccctgca ccgtagagtg cgaccagccc caccaagatt cccaggtagt
     8941 aaatcgtcag ggccacgaac cggacccatg gtcgcgcgaa ccattcgctc aacgggccat
     9001 tatctttgcc aagttgatct ccaagactca tggctttccc tttcgtccct ggaatgttgg
     9061 ttcggggttc gtccttggcg cccggacttc tatcgccgtc gcggaggacg gatcgagcaa
     9121 accttcggag ggaggatggg acccgggacc cggcggcgac agcagaccaa cttcggtcgc
     9181 tctgctcgcc acgggcgcag aggcgggcag ctcgggctgg cttgggatcg cgtcgtggaa
     9241 gaaactgtcg aggatttggc tgtaatacac caagccacgg ggagctaggt gtcccaagtt
     9301 atcatggcag aaagaccgat cggcatcgtg atcggcgaaa tcgacgaccg cagcgtgata
     9361 gcgggcaccg gtcgcgcgta attcctggta gtaggccctt cgggcggtgt atgtcacccc
     9421 acactggtcg aaccagccgc cgtggatcgg catgctcaag aggagcggct gggcaccgag
     9481 ttcggtcaac tcgcgcagga gaagttccag atcgacccat tcctgggttt tttggagtgt
     9541 gaggagaaaa gccttctccg accgggtgtt tctttccagg aacatttctt ggcgaagttc
     9601 gcggttccac ttttcgttat ccagcccgaa ctcattgttg ttggaatgag cactgtacaa
     9661 tgcgtctgcc tgccggtgca acgtcggcca atctaatggc cggtcgctgc gcggcgaaat
     9721 cggggacgag gtccttatgg ggtgtttcca gagataatac accacgctcc agtgatccag
     9781 attgcggagg atcgcattgt gcacgattcc caaggggaaa accgcttgat agcaggcgag
     9841 gcccaggggg gaaccgtcgg ccatgttctc cagggcgaac ctgaggagcg gtcgattcgc
     9901 cacggtttct gggtattgga gcatccgccg cgccgcgtcc tgcctgagct ggagactcaa
     9961 gcgggtattg aaggccaatt ctccggcgtg caagggtgag aaattgccag cgtatccgtc
    10021 cgcccgaacc gtgagccaat cgaaaaacca aaaaggcgac agggagaccg ccactttccg
    10081 ccccttgagc gcggggccga ccgcggccag cttttgcagg atgatcaagc aagtcgttgc
    10141 cgccttgccc accgggaaga ccgtgaaccc cgtgggctga tcgtggaaca agttggttgc
    10201 atgaaaaggt cgattgtaaa ccgcttgcag gttcagctca gagctgccgt aaatcggcag
    10261 caagcagtcg gtgtccaggg ccgcctgttg caaggcagtg ccttggttct ttacgggggc
    10321 cagtttgccg tttcgttcga tgattgcctc atccgccgcg agcgccttga tcgagcgata
    10381 ttccaaagac cgggcataga acccgaccgc tgccatcagg acgacgccca gaatcaccgc
    10441 cgtcagagca ggtgcgaggt ggggggtttg ctccaagggc tgatgctgac catgcatcat
    10501 cgtctctctc gaccggttct tatgcgaagg ctcgctggat ccgatgccgt ttcctcttta
    10561 tcggatgctc cggccccgag agttcaatgc tcgcgactgc cattgccttt cctgaacgac
    10621 cttgttctcc gggttttcgc tcatgaggtt acatgttgac tgccgaagct atcgatcata
    10681 ggaaataccc ggttaagcgc ccggcgagct ccctggatgg aaagacaggc gagatagcaa
    10741 taatggcagc cttgtcctcc ttctgatcga tccatatacc gcagatcaat aagcaatgac
    10801 gaccggcaaa cctcaactgc gtaacttcta attcaatccc cgctatttac aaggagactt
    10861 tccagcaatc tgggcggtac cctgcattcg ccattagctt cggatggcgc acggccagcc
    10921 ctcggcctca acgacttacg aggacaagca tgatgcgtct cttcgcacga cggcaggatt
    10981 cacgcccttc gtccgagcct tgcaccggat cggcccagtt ccgggcggcc gatcggccct
    11041 gccagcgcgc gcgccggcgg gcgcggcccg aggccgagat gctcgatacg cgcctcctca
    11101 tggcttccgg ccaacttggt atggccgacg cgaccgcgtc gcccgatctg gccgaccgcg
    11161 tcgcgcaggc cgtgaagccc tacctcgctc agaaccaggt ccccggcatc tccgtggcgg
    11221 tggtcacgga cggccaggtt gcgctggcgc aaggctacgg gaacagcaac gtggcgaccg
    11281 gctcacccgt ccaagccgac acccgcttcg acatcggttc ggtaacgaag acgttcaccg
    11341 cccttggggt cctgctcctc taccaagaga gccagggcac gtcccacccg ctcaacctcg
    11401 atgcccctat cagtcaatat ttgcacaata ataaatcttt taagctgccc ccaaaatggt
    11461 cccgtatcac aacgagggag ttgctcgaca tgacgagcgg catcagagat gtggggggcg
    11521 cccagccctg gcaggcccag cttgcgtcga tcgcgaaata ccccctgctc tacgcccctg
    11581 ggacggagag ctcgtacagc agcgcgaatt acgacctgct cggcgagctc atcgagcagt
    11641 ggaccggcga aacgtacggc acctacatcc aggatcagat cttgggaccg ctcgggatga
    11701 ccgggactca ggaactgggc cggtccgcta cagtcccgaa ccaggcggtc ggctacggcg
    11761 cgcccaggcg cgggaagtgg acgaaagccg cggtacagaa cggaccggcg ttgtacgccg
    11821 ccgccgggat ggtctcgacg gcccaggaca tggccactta catgacggcc ctgctgagcg
    11881 accacctcct cgaccccgcg accacccggt tgatgtggac cgggataccg accccgcaac
    11941 acgggccggg gggcccatcc accggcgtgc ccggcctggg ctgggatacg gcgatcgaca
    12001 cgagcgacgg gcccgtcgcg atctacaaga gcgggtcagt cccc
//
LOCUS       DATAQL010000452         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4319223, whole genome shotgun sequence.
ACCESSION   DATAQL010000452 DATAQL010000000
VERSION     DATAQL010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4319223"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1178)
                     /locus_tag="VHS97_07810"
     CDS             complement(45..1178)
                     /locus_tag="VHS97_07810"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX3448146.1"
                     /translation="MKGKTFWVLLLAFVAGGGVLAWQAWQTWAAALPPGIASGNGRVE
                     SVQVDVTTKEPGRVLTILVREGDIVRPSQIVAKMDTVTMESELARAEATVAEQEAKLF
                     EVEAAIAKADSQLKLDEIEFARTRALLARNAVSREEFDVKQTLVKTSGATLNGEKARL
                     ETARKTIVAAKAEVAYIQSRINDMTLRSTVQGRVLYRLAEEGEVLGTGGKVLTLVNLG
                     DVYMEIYLPAQDAMRAKIGADARIVFDIVPEYAARAKVSFVAPEAQFTPKQVETSSER
                     DKLMFRVKLAVPPERVLQYIERVKTGIRGIGYVRLDDSVEWPERLNRQLPEPGSTSPA
                     KRALQPAAAGPSATSAEAQPTSVPTQEKSATPKAAEGSSSNRN"
BASE COUNT          361 a          553 c          513 g          363 t
ORIGIN      
        1 tcggacggga tcggcagaag cgctagggaa agtcatcaaa ttctctaatt acgatttgag
       61 gacgatccct cggcagcctt cggcgtggcc gacttttcct gcgtcggcac cgatgtcggc
      121 tgcgcctccg ccgaagtggc cgagggcccc gcggccgccg gctgcaatgc gcgcttcgcc
      181 ggagaagttg acccgggttc cggtaattgc cggttgagtc gctctggcca ctcaacagag
      241 tcgtcgagtc ggacgtaacc gatgccacga ataccggtct tcactcgttc gatgtattgc
      301 aggacgcgct cgggaggcac cgccagcttc acgcggaaca tgagcttgtc acgctcgctg
      361 ctggtctcaa cctgcttggg tgtgaactgg gcttccggcg cgacaaaact gaccttcgcc
      421 cgtgccgcgt attcgggcac gatgtcaaaa acaatgcggg catcggcgcc gatcttggcc
      481 ctcatcgcgt cttgggccgg caggtagatc tccatgtaaa catcgccaag attgacgagc
      541 gtgagcacct tgccgccggt gcccagaacc tcgccttcct ccgccagtcg atagagtacg
      601 cgcccctgaa ccgtggagcg gagagtcatg tcgttgatcc gcgactggat gtacgccacc
      661 tcggccttgg ccgcgacgat ggtcttccgg gccgtttcga gccgggcctt ctcaccgttt
      721 agcgtggcgc cggacgtctt cacgagggtc tgcttgacgt cgaactcttc ccgggagacg
      781 gcgttacgcg cgaggagagc gcgggtgcgc gcgaactcaa tttcgtccag cttcaactgg
      841 ctatcggcct tggcaattgc ggcttcgacc tcgaacaact tggcctcctg ctcggcgacg
      901 gtcgcctcgg ctcgggccag ttctgactcc atggtgacgg tatccatctt ggcgacgatc
      961 tggctgggcc ggacgatgtc cccctcgcga acgaggatcg ttaaaactcg gcccggctcc
     1021 ttggtcgtca cgtcgacctg gaccgactca acccgcccgt tccccgaagc gatccctgga
     1081 ggtagcgccg ctgcccatgt ctgccaagcc tgccaggcga gaaccccgcc gccggcgacg
     1141 aaggccagca gaaggaccca gaatgttttc cccttcattg cggcccctcc caacgaatca
     1201 ttgactcgat cgcgttgatc gaacaccact ctagccacta ctgggccatg ccgccaatct
     1261 tgggactttt taacgaatcc gacgactttt tagcgaatcc gacatggcaa tcacgcttca
     1321 ggaacgagaa tacccatcaa atcctcgcag cagaagccat tccatatttg acaggtgaaa
     1381 acttaaccga cttccaagtg acaaagaggt ggccgtcgct ggcgtcgaaa tccgcaatcg
     1441 agtgaaaata gggagcggag gttctttcac cataagtgac aatatgatag ctttcggacg
     1501 ttggcggtcg ctcccgaacc aggaatctgc catttccgat gatcgcttga tatcgcgcgt
     1561 ctgagctgcc gctggggatt tttctatcca actcgggcga cgagtcctcc ggctcgattg
     1621 gcgtgcccct caccgcgctc cggcataatc ttgtagtgaa aggacttgac gtgagccgcc
     1681 gcgacaagaa aacggtagaa aacgccctcg cggcgcatga gaaactcaaa gacaaagagt
     1741 acttgggcga actcaaaaag ctgcacgtgg agctggtcaa gctccaggag
//
LOCUS       DATAQL010000453         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2029933, whole genome shotgun sequence.
ACCESSION   DATAQL010000453 DATAQL010000000
VERSION     DATAQL010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029933"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VHS97_07815"
     CDS             <1..567
                     /locus_tag="VHS97_07815"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX3448147.1"
                     /translation="PYVETDATTPLSIYGRSKRDGEVAVRACKWHLIFRTSWVVGAHG
                     HNFLRTILRLAKDRPNLRVVADQHGAPTSAALLAEVTARVLAQTGQSTAEPRWGLYHL
                     VAGGETSWHGVARHAIARANTMGLPLLASPETVEPITTADYPTPARRPANSRLSTAKL
                     RETFALTLPDWSVGVNAVLDQIIPEMSK"
     gene            564..1451
                     /gene="rfbA"
                     /locus_tag="VHS97_07820"
     CDS             564..1451
                     /gene="rfbA"
                     /locus_tag="VHS97_07820"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX3448148.1"
                     /translation="MTAGDSIRRKGIILAGGSGTRLYPVTQCVSKQLMPVYDKPMIYY
                     PLTTLMLSGIRDILVISTPHDTPKFSELLADGSQWGVNIQYAVQPNPGGLAQAFIIGR
                     DFVGGELSTLILGDNIFYGHDMVKLLLSAHARREGATVFAYHVQDPARYGVVEFDARQ
                     RAISIEEKPEAPKSNYAVTGLYFYDSHVCDIAADIKPSARGELEITDVNRRYLDSGLL
                     NVETMGRGYAWLDTGTHDSLLEAASFVATLQRRQGLIVACPEEIAYGQKWIDAGQIAT
                     LAHPLSKTGYGQYLLRLIQ"
     gene            1479..>1576
                     /locus_tag="VHS97_07825"
     CDS             1479..>1576
                     /locus_tag="VHS97_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX3448149.1"
                     /translation="MPTSLPGVLLLEPKVFGDPRGFFFESFNARDFG"
BASE COUNT          321 a          498 c          461 g          296 t
ORIGIN      
        1 ccttacgtcg agacggatgc aaccactcca ctttccattt acggccgcag caagcgcgac
       61 ggcgaggtgg ccgttcgtgc ttgcaagtgg cacttgatct ttcggaccag ctgggtggtg
      121 ggtgcgcatg ggcacaactt cctcaggaca atccttcgac tggcgaagga tcgacccaac
      181 ttgcgcgtgg tcgccgacca gcatggcgca ccgacctcgg cggcgctcct cgccgaggtc
      241 accgctcggg tgctggcgca aaccgggcaa tcgacagccg agccacgctg gggcctctac
      301 catctcgtcg ccggcggcga gaccagctgg cacggcgtgg cacgtcatgc gatcgcccgg
      361 gcgaacacca tggggctgcc gttgctggca tcgcctgaaa ccgtcgagcc gataacgacg
      421 gcggactatc caacgccggc aagacgcccg gcaaattccc gcctgtccac ggccaaactt
      481 cgcgaaacgt ttgccttgac actcccggac tggtcggtgg gtgtaaatgc tgtcctcgat
      541 cagatcattc cggagatgag taaatgacgg ctggcgactc aattcggcgc aaaggcatta
      601 ttctggccgg cggctccggc acgcgcctct acccggtcac gcaatgcgtg tcgaagcagt
      661 tgatgcccgt ctacgacaag ccgatgatct actatccgct caccaccttg atgttgagcg
      721 gaatccgcga tattctcgtg atctcaaccc cgcacgatac gcccaagttc agcgagcttc
      781 tggccgacgg cagccagtgg ggcgtcaata ttcaatatgc cgtccagcca aaccccggcg
      841 gcctcgcgca agctttcatt atcggtcgag attttgtcgg cggtgagctg agcacgctca
      901 tacttggaga caatatcttc tacgggcacg atatggtgaa gctgctgctc agcgctcatg
      961 cgcggcgcga aggggccacg gtgttcgcct atcacgtcca ggatcccgca cggtacggcg
     1021 tggtcgagtt cgatgccaga caacgagcca taagcatcga agagaaaccc gaggcgccga
     1081 aatcgaacta tgcggtgacc gggctttact tctacgactc ccacgtttgt gacattgcgg
     1141 ccgacatcaa gccatcggcc cgcggcgagc tggagatcac cgacgtgaac cgccgttacc
     1201 tcgatagcgg cctgctcaat gtcgaaacca tggggcgagg ctatgcctgg ctcgacaccg
     1261 gaacgcacga tagcctactg gaagccgcga gcttcgtcgc caccctgcag agacgtcagg
     1321 gcctgatcgt ggcttgcccc gaagaaatcg cctacggaca gaaatggatc gacgccggac
     1381 aaatcgcgac cctggcgcac ccgctcagca agaccggcta cgggcagtat ctgctcaggc
     1441 tgattcaatg aaagaccgcg actatgcctt ataaggtgat gcccacgtca ctgcccggag
     1501 tgcttctgct ggagcccaag gtctttggcg acccgcgcgg cttttttttc gaaagcttca
     1561 acgcgcgcga tttcgg
//
LOCUS       DATAQL010000454         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2029971, whole genome shotgun sequence.
ACCESSION   DATAQL010000454 DATAQL010000000
VERSION     DATAQL010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029971"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VHS97_07830"
     CDS             <1..751
                     /locus_tag="VHS97_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3448150.1"
                     /translation="TVVAALPLAPAPKALPDLAATANTPGAGTGFLDKLYRDANGTEV
                     KYAVFVPPDYTGNNTVPAILYLHGAGRRGTDGRSHIVSGLAKAIREKNEDFPFLVIFP
                     QAREDENWTPESTGGKRALAILKQVQSEYRVDPNRVALTGVSMGGQGTWSLAAAEPDR
                     WSAIVPICHGWKPNLAARLKDLPCWCFHGDADQVIPASQSREMVQAIQQAGGKPLYQE
                     FIGVDHNQCADHAYALPELYDWLLLQDRARR"
     gene            complement(912..>3730)
                     /locus_tag="VHS97_07835"
     CDS             complement(912..>3730)
                     /locus_tag="VHS97_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HEX3448151.1"
                     /translation="SSSPRTGSASQWAGAQAPPATAVRSVTTPKQFFGFQIGEDYSLA
                     NYQQLKGYWEKLERESDRIKVVNIGPTEEGRPQLVAIVTSAASHRRLDRHQQIARRLA
                     LADGLDQTEAHKLAVEGKAVVWIDAGIHATETLCPQAMIETVFQLLAGTDDETLRLLD
                     DVIILFVHANPDGHDLVADWYMREKDPGKRSLAGLPRLYQKYIGHDNNRDFYANTQAE
                     TKNLNRLMYREWFPQVMYNHHQTGPAGTVLFCSPFRDPFNYFCDPLVVNGIDAVGAAM
                     VERFLAEGKPGATVRSGGPYSTWFNGGIRTTTSFHNIIGVMSETIGSPTPTRIPLVAA
                     KQLPKGDYLAPIPPQPWHFRQSVDYSVTANKAVLDYASRHREQLLYNIWLMGHNAIER
                     GNRDSWTITPKVVASAQGGRTAASAQGAGRAGPDRRGPDQVRSGSATDFERLFHEPSR
                     RDPRGYILPADQTDFLTATKFINTLLATGVRVHRAAADFTVANRSYPQGSYVVKTSQA
                     FRAHVLDMFEPQDHPHDLAYPGGPPVRPYDSAGYTPAFQMAVKFDRVLDGFNGPFEEI
                     KELAVAPPPARVFESEGAAGFFLGTETNDSFRAVNRLQKAGEEVRRLQRPLAVGGVAH
                     PAGTFFVPSTSTTLPVLEKIAAEIGTPFRGSTVAPGPDAVALKTPRIGLWDRQGGSIP
                     SGWTRWLLEQFEFPFDVVFVPELDKGGLRDKLDVLILVDGAYAVRGGPNGAGAGSGDR
                     PDDGGAAGASERSGNNPARDQRGSITTTATLPQLKKFLEDGGTLLAIGSSARLGHELG
                     LPIANHLAALDKDGREQPFPPEKFYVPSSVLRIKVDPSNSLAWGLRDEVDVMISSSPT
                     FRLTDQTKTNLVRRVGWFDNKSPLRSGWAWGQEYLEGGIAIVDSTIGKGRLALFGPQV
                     LFRGQPHGTFKFVFNGIVQSAVRF"
BASE COUNT          679 a         1141 c         1175 g          735 t
ORIGIN      
        1 aacggtcgtc gcggccctgc cacttgcgcc agccccgaag gcactcccgg acctcgcggc
       61 aacagccaac acgcccggag ctggcactgg cttccttgac aagctttatc gcgatgcgaa
      121 cggcaccgag gtgaagtacg ccgtgttcgt cccgcctgac tacaccggca acaacaccgt
      181 accggcgatt ctgtacttgc acggcgcggg gaggcgcggc actgacggcc gaagtcacat
      241 cgtcagcggt ctggcaaagg ccatccgaga gaaaaacgag gactttccgt tcctcgtgat
      301 ctttccgcaa gcccgcgaag atgagaattg gacgccagag tccactggcg gcaaacgcgc
      361 cctcgcgatc ctgaaacagg tgcaatccga atatcgtgtc gacccgaatc gggttgcact
      421 cacgggcgtt tccatgggcg gccaaggaac ctggagtctc gctgcggcag aaccggatcg
      481 ttggtcggca atcgtgccga tctgccacgg ctggaagccg aatctggcgg cgcgtctcaa
      541 agacctgccc tgctggtgct ttcatggcga tgccgatcaa gtcattcccg cgtcgcagtc
      601 gcgtgagatg gtccaggcga tccagcaggc cggtgggaag ccgctctatc aggagtttat
      661 cggcgtcgat cacaatcagt gcgccgacca cgcttacgcc ttgcccgaac tgtacgactg
      721 gctgttgctc caggatcgcg ccaggcggta gccgcttcac ggaaaagtcg gcgtgcggtc
      781 ggatgcgccc acggattctg gcaatcgggt tacgctttgt gtcaccgcga tgcgctgtct
      841 ccgccgttgg agaccttcgg tcgcgccgta gggcggggtc ggagacccgc gaccgtcttg
      901 ccgatatgtg atcagaacct gacagcagac tgtacgatcc cgttgaagac gaacttgaat
      961 gtcccgtgcg gctgaccgcg gaacagaacc tgcggcccga ataatgcgag ccggcccttg
     1021 ccgatcgtgc tgtcgacgat tgcgatgccg ccttcgaggt actcttgacc ccaggcccat
     1081 ccactgcgca acggggattt gttatcgaac caacccaccc gccgcaccaa gttcgtcttc
     1141 gtttggtctg tcagccgaaa cgtcgggctg gatgaaatca taacgtctac ctcgtccctc
     1201 aagccccagg cgagcgagtt cgacgggtcg acttttatcc ttaacaccga cgacggtaca
     1261 tagaacttct cgggcggaaa gggttgttcc cggccgtcct tgtcgagcgc ggcgaggtga
     1321 ttcgcgatcg gtaatcccaa ctcgtgcccg agccgcgccg aactgccgat ggcaaggagg
     1381 gtgccgccat cttcgaggaa ctttttcaat tgtggtaagg tggccgtcgt ggtgatactt
     1441 ccgcgttggt cacgggctgg gttattgccc gatctctcgc tggctccggc cgctccgccg
     1501 tcgtcaggcc ggtcgccgct gccggcgccc gcaccgttgg gtccgccccg aaccgcgtac
     1561 gccccgtcga ccagaatcag cacatcgagc ttgtcgcgca atcctccctt gtccagttcc
     1621 ggcacgaaga cgacgtcgaa cggaaactca aactgttcca acaaccagcg ggtccaaccc
     1681 gaaggaatgg atccgccttg cctgtcccaa agtccgatgc gaggcgtttt gagcgcaacg
     1741 gcgtccgggc ccggcgcgac ggtactgccc cgaaaaggcg tgccaatctc cgcggcaatc
     1801 ttttccaaca ctggcaatgt cgtcgacgtg ctcgggacga agaacgttcc cgccgggtgc
     1861 gcgacgcctc cgactgcgag gggccgctgc aaccggcgga cctcctcacc cgccttttga
     1921 aggcggttca cggctcgaaa cgagtcgttg gtttcggtac ccaggaaaaa gccggcagcg
     1981 ccctcggatt caaacacgcg tgctggcggt ggtgcgactg cgagctcttt gatttcctcg
     2041 aacgggccgt tgaagccgtc gagcacccgg tcgaatttca cagccatctg aaaggccggc
     2101 gtgtatccgg cggagtcata tggccgtacc ggagggcctc cggggtaggc taagtcgtgg
     2161 gggtggtcct gaggttcaaa catatccagc acatgggcgc ggaacgcctg ggaagtcttc
     2221 acgacgtacg agccttgggg atatgacctg ttggcgacgg taaagtcggc cgcggccctg
     2281 tggacccgga ctccggttgc gagcaaggtg ttgatgaact tcgtcgccgt caaaaagtcg
     2341 gtctgatcgg ccgggagaat atagccacgg gggtcgcggc gtgacggttc gtgaaagagg
     2401 cgttcgaagt cggtcgcact gccactcctc acttgatccg gcccgcgtcg gtccgggcca
     2461 gcgcggccgg cgccctgagc cgacgccgcg gtccggcccc cctgcgcgct cgcgacgact
     2521 ttgggggtga tcgtccaact gtcacggttg ccgcgctcga ttgcattgtg gcccatcaac
     2581 caaatgttgt agaggagctg ctcccgatgc ctcgaggcat aatcgagcac cgccttgttg
     2641 gccgttaccg aatagtccac cgactggcgg aagtgccatg gctgtggcgg gatcggagcg
     2701 aggtaatccc ccttgggaag ttgcttcgca gccacgagcg ggattcgtgt cggcgttgga
     2761 ctgccaatcg tttcactcat cacaccgatg atgttatgga acgaggtcgt ggtgcggatc
     2821 cctccgttga accaggtcga gtatggtccc cccgagcgga cggtggcacc cggctttcct
     2881 tcggcgagga agcgctcaac catcgccgcg ccgacagcgt cgatgccgtt aacaaccagc
     2941 gggtcgcaga aatagttaaa cggatcccga aacggcgagc aaaacaagac ggtgccggcc
     3001 gggcccgtct ggtgatggtt gtacatgacc tggggaaacc actcacggta catcaaccgg
     3061 ttgagatttt tggtctcggc ctgagtgttg gcatagaagt cgcgattgtt gtcgtggccg
     3121 atatactttt ggtagagccg cggcagaccc gcaagcgagc gcttgccggg gtctttctcc
     3181 cgcatatacc agtccgcgac gaggtcgtgg ccgtcggggt tggcgtggac gaataggatg
     3241 atcacgtcgt cgaggaggcg gagcgtctcg tcgtcggtcc cggcgagcaa ctggaagacg
     3301 gtctcgatca ttgcctgtgg gcaaagtgtc tctgtggcgt gaatgccggc gtcgatccac
     3361 accaccgcct tgccctcgac cgccagcttg tgggcctcgg tttggtctag tccgtcggcg
     3421 agggccaggc ggcgcgcgat ctgctgatgt cggtccagtc ggcggtggct ggctgcggag
     3481 gtaacaatgg ccacgagttg cggccggcct tcctccgtcg gaccgatatt gaccaccttg
     3541 atccggtctg actctcgctc gagtttctcc cagtaacctt tcaactgttg ataattcgcc
     3601 aggctatagt cctcaccgat ttggaagccg aagaattgtt ttggcgtcgt gacggagcga
     3661 acggccgtcg cgggcggtgc ttgcgcgccg gcccattgtg acgcgctgcc ggtccgtgga
     3721 gaagacgatt
//
LOCUS       DATAQL010000455         7596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1877903, whole genome shotgun sequence.
ACCESSION   DATAQL010000455 DATAQL010000000
VERSION     DATAQL010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877903"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VHS97_07840"
     CDS             complement(<1..1111)
                     /locus_tag="VHS97_07840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX3448152.1"
                     /translation="MDRRSFLAAAAALTGSSPSRPAPASDGGKPVRTQPLRASFYGPE
                     YYDDKELFELRDVLEKRLPFRWYGPGRQPPMKVLTFEKELAARMQTKFALAVTSGTAA
                     LTTALAALGVGPGDEVILPALSWYSCFNSIVMHGALPVFAESDESLNVDPADLEAKFT
                     PQTKVIMVVHALGNPADMDTIMAIARRRDIKVLEDCAQSLGGSYKGKPLGSIGDIGIY
                     SFQLSKTISSGEGGAVVTSDPLLFERASRYHDLGILRPPHEQILGGAALKGMIGSQYR
                     MSEFTGGVLLAQLKKLDTIIGSLRGHAKRVYEGLADLPNLQSRQRPDPVGDLGSGIWL
                     GFPGPVERDRFLAAMRAENVPAGAPSAVALVPLHPS"
     gene            complement(1431..4556)
                     /locus_tag="VHS97_07845"
     CDS             complement(1431..4556)
                     /locus_tag="VHS97_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3448153.1"
                     /translation="MREPLVTKLTCGLIVAVFAAAPLHAQSVPKERQITHREQSLTPY
                     DPFSADELFKRIKIPPSPALTPAEALKSFQVAPGFRIECVAAEPLVVNPVMFEFDPDG
                     RIWVVEMRGYMRDLEGSDEGTPIGNVVVLEDTNGDSVMDRSTVFLDNLVMPRTLSFVA
                     GGLLVAEPPNLWYCRDIDGDLKCDEKRLVGTYGRPGNPQHTDNGLFHALDNWLYSADC
                     TFRHKFIDGKLVQEPMARRGQWGVTQDDYGRLFYNYENSSLHADLIPEEYTRRNPHLT
                     PETGRQNSNSGLNVNLAEMAHEIFTIRVNPGITLGGTELRPDGRLRTFTIACGPSIYR
                     GDQFPSEFYGGAVIPEAGGNLVRFDLLAGDGVHVAARNAFDQREWIASTDERFRPVCS
                     RTGPDGTVYICDMYRGIIEHVIFMMPYLRNQILSRGLEMPYDGGRIYRIVHESKPLGP
                     APRMSGASSPQLVKWLSHPNGWWRDTAQRLLVEREAVEVTDLLRELAATGQSNVGRLH
                     ALWTLEGIDRLDWPTVKAAMSADDSMVRATAIRLSERFRDPVQHAATFEQIERYFADA
                     RPMVQRQLLLTLGHFPGSDPAERQMAAILGRWPDDIFLVAAASGLKSRELEMLTRLVE
                     SAGWTANHERRCGAIETLSMCVVHEGNSTRVERLLGLAADLAPAQVWATDSIVAGVLK
                     SNKSRSRWPDPIVLSERPRLLTVKPSSTDALVRIITWPGDVTVRDRRPVLPPLTPAQE
                     KRFALGEAVYNVTCIACHKEDGRGQPAQAPTLVDSEWVNGPHDRLARIVLDGIQGPVK
                     VNGEDWNLKMPGLGNSPVMSDERLAGVLSYVRRKWDNYGSAVEPEQIAAIRRSTSDRA
                     TPWTVEELLDPSKIATRSAASKTDPLEPYRTLLASGDAEKGRFLFHSNREIRCNACHT
                     VGNLGGGFVGPDLTEVGKRANRDYLLESLIDPSSKIVKGFETLVIVTDDGKIFSGTFV
                     ADDGRTLVVAPAGGGKVEIAIDKIAERVQSPVSSMPPMANTFTPQQIADLVAYLESLK
                     PAPSAGELGKP"
     gene            complement(4857..5207)
                     /locus_tag="VHS97_07850"
     CDS             complement(4857..5207)
                     /locus_tag="VHS97_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3448154.1"
                     /translation="MDVSVNGGAFTKLSTINLPSVTFVDTDLTFAPITVTSSLDIRFE
                     VTPGSSAANGGAIGSAGTWRISDDSPDNGATFLPVQLVGSVVPEPSSFVLAGIGLAVA
                     AVVSRVRREKPVYG"
     gene            complement(5350..6999)
                     /locus_tag="VHS97_07855"
     CDS             complement(5350..6999)
                     /locus_tag="VHS97_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3448155.1"
                     /translation="MGRRQLPSALRQLRALFAAGTATGLSDSELLERYTAKRAQSVEA
                     ATAAELAFEALVDRHGPMVWGICHRVLGDAYEAEDAFQATFLVLVRKAGSVRVNGSLG
                     RWLYGVAHRIALRARSEAERRRSCMGSAPVVSSDDPVGEVERKDLRKAVGEELDRLPG
                     KYRCAVELCHLQGMTYDQAARQLNCPVATVKSRLTKGRLRLRERLARRGIAPGAVGLA
                     TALTRKARAAIPQKLVQSTVRAATSCAKGAFPAAVTDLTEGVLKMMMWEKLKLVVPGA
                     LVAIGLTAQALSQPAPNGGIRPARPPQTAAQPAEKPNEKQAGDRRWVRSLPSGALIEI
                     IGVSSFPSGPDTWWRPDGTPLHPAPCDPIRPGISGDTGVLKLVLVRLARIPDGADHEW
                     SIAEARGAAVGPAMRDGKPLPGLSETAALLQADAGTGTVRFKVAAGPWKTIVTQGKNS
                     VAVGSAIGPSYIFSGAIATKEGAALSVIHNIQDKPVRLVAVDVDGKILPAAVRSGNGV
                     KDFRQLVVEFDQPPEQIKEFRLQSRPYEEVRIPGIALKRRP"
     gene            7158..>7596
                     /locus_tag="VHS97_07860"
     CDS             7158..>7596
                     /locus_tag="VHS97_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX3448156.1"
                     /translation="MASNSSGPALRTIRALFTAGTIGGLTDGELLERFTARDGDGAEL
                     AFASLVERHGPMVLRVCRTLLRDAHLADDACQATFVILALKAGSIRGQHSLTSWLYSV
                     AYNVAATARSSSARRRAHELKAAQARPLTYIEDAPDELGPVIHE"
BASE COUNT         1425 a         2370 c         2353 g         1448 t
ORIGIN      
        1 ccgagggatg gagcggcacg agcgcaactg ctgacggcgc ccccgccggc acgttctcgg
       61 cgcgcatcgc ggccaggaat cgatcgcgct cgaccggccc ggggaatccg agccagatgc
      121 cggaaccgag atcgccgacg gggtccggcc gctggcgcga ctgcaaattg ggcaggtcgg
      181 cgagcccctc gtaaacacgc ttggcgtggc cgcgaagcga accgataatc gtgtcgagct
      241 tcttgagctg cgccaagagc acgccgccgg taaattcgct catgcggtat tggctgccga
      301 tcatcccttt gagtgcggcc cctccgagta tctgctcgtg aggcggacga agaattccca
      361 ggtcgtggta ccgcgacgcc cgctcaaaca gcaatggatc gctcgtgacc acggcgcccc
      421 cttcgccaga gctgattgtc tttgagagct ggaagctgta aattccaatg tcaccgatcg
      481 atcccagcgg cttgcccttg tagctgccgc ccaggctctg agcgcagtcc tcgagcacct
      541 tgatgtcacg cctgcgggcg atggccatga tggtgtccat gtcggccgga ttgccgagcg
      601 cgtggacgac catgatcact ttcgtttgcg gcgtgaactt tgcctcgagg tcggcggggt
      661 ccacgttcaa tgactcgtcc gactcggcga acaccgggag ggcgccgtgc atcacgatcg
      721 agttgaagca ggagtaccag gagagggccg gcaagatcac ctcatcaccc ggcccgacgc
      781 ccagcgccgc caacgccgta gtcagtgcgg ccgtccccga ggtgaccgcc agggcgaact
      841 ttgtctgcat ccgcgcggct agctctttct cgaaggtgag caccttcatt ggcggctgcc
      901 taccgggccc gtaccagcgg aacggcagcc gcttttccag gacgtcccta agctcgaaga
      961 gctctttgtc gtcgtagtac tccgggccgt aaaaactcgc acgaagcggt tgggtacgga
     1021 ccggcttgcc gccgtcggac gccggcgccg gccttgatgg gctcgagccg gtcagagccg
     1081 cggcagccgc caggaatgat cgacgatcca tggggcctcc tttgccagag cgtagactta
     1141 ctgcgccgct ctagcaaggc tcgaggcaaa acagttagtt catcggtaac ttatataata
     1201 atagcacgtt ataatttcct ccccacatgt ggcggagggc cagggtgggg ggcgttcctg
     1261 tctgggaccg tagccgtgcg aagtcttcgg ttcccgcctc cccccacccg aaccctcccc
     1321 cacaagtggg gaggtcgaca tagatgagcg gggacgagta agtaacacca gcttcagttg
     1381 cactgaagct ggatgtggtc gtcaaattcg cttgcgccgc tcgtaagacg tcatggcttt
     1441 cctagttcac ctgccgatgg tgcaggcttg agcgattcca gataggccac cagatcggca
     1501 atttgctggg gcgtgaacgt attggccatt ggtggcatgc tcgagaccgg cgactgaacg
     1561 cgctcggcga tcttgtcgat cgctatctcg acctttccgc ctccagccgg agcgacaacg
     1621 agggtacgac cgtcgtcggc aacgaatgtc ccggaaaaga tcttgccgtc gtcagtgaca
     1681 atgacgagcg tctcgaaccc tttgacgatc ttggagctcg gatcgatcag cgattcgagc
     1741 agatagtcgc gattggcacg tttgccgact tccgtcagat cgggcccgac gaacccgccg
     1801 ccaaggttgc ctaccgtgtg gcaagcgttg cagcgaatct cacggttcga gtgaaataga
     1861 aatcgtccct tctcggcgtc tcccgaagcg agtaaggtgc gatacggctc caatggatca
     1921 gtcttcgagg cggccgacct ggttgcgatc ttcgacgggt cgagcagttc ttcgaccgtc
     1981 cacggcgtcg ctcggtcgga cgtcgatcga cgaatcgcgg cgatctgttc cggctcgaca
     2041 gccgaaccgt aattgtccca tttccggcga acgtacgaga gcacgccggc gaggcgttcg
     2101 tcgctcatta ctgggctgtt gccgaggccc ggcattttca gattccaatc ttcaccgttc
     2161 accttcaccg ggccttgaat tccgtccagg acgatgcggg cgaggcggtc gtgtgggccg
     2221 ttgacccact ccgaatcgac gagtgtcggc gcctgagcgg gttggcctcg cccgtcttcc
     2281 ttgtggcagg caatgcaggt gacattgtag acagcttcgc cgagcgcaaa ccgtttttcc
     2341 tgggcaggcg ttagcggcgg cagcaccggc cggcgatcgc gcactgtgac gtcgccgggc
     2401 cacgtgatga tccgcaccag cgcgtcggtc gacgacggct ttacggtcag caggcgcggg
     2461 cgctcactga gcacgatcgg atccggccag cgactgcgac tcttgttcga cttcaatacg
     2521 cccgcaacaa tcgagtctgt ggcccagacc tgggcaggcg ccaggtccgc ggccagcccg
     2581 agtagccgtt ctacacgggt cgaattcccc tcgtggacaa cgcacatcga cagggtttcg
     2641 atcgctccgc atcgccgttc gtggttggcg gtccatcctg ccgactcgac caggcgcgtg
     2701 agcatttcga gttcgcggct cttgagtcca ctagccgccg cgacgaggaa gatgtcgtcg
     2761 ggccagcgac cgaggatcgc cgccatctgt cgttcggccg gatcggaacc cggaaaatga
     2821 ccgagggtca gcaggagttg ccgttgtacc attggccgcg catcggcgaa gtaccgctct
     2881 atctgctcga aggtcgcggc gtgttgcacc ggatcgcgga accgttcact caggcgaata
     2941 gcagttgctc gcaccatcga gtcgtcggcc gacattgcgg ccttgaccgt cggccaatcg
     3001 aggcggtcga ttccttcgag tgtccagagg gcgtgcaacc gtcccacatt gctttgccct
     3061 gttgccgcca gctcgcgcag caggtcggtc acctcgaccg cctctcgttc caccagcagc
     3121 cgctgcgcgg tatcacgcca ccaaccgttt ggatgtgaga gccatttgac gagctgtggc
     3181 gacgatgccc cagacatccg tggcgccgga ccgagcggct tgctttcgtg cacgatccga
     3241 tagatccgcc ccccgtcata cggcatttcg agtccgcgcg agaggatctg gttccgcaaa
     3301 tagggcatca tgaagatcac gtgctcgatg atgccgcggt acatatcgca gatgtacaca
     3361 gtcccgtccg gccccgtgcg actgcagact ggccggaacc gctcgtccgt cgaggcgatc
     3421 cattcccgct gatcgaacgc attgcgggct gcaacgtgca ccccgtcgcc tgcaagcaga
     3481 tcaaatcgca ccaggtttcc gcccgcctcg gggatgacgg cgccaccgta aaactcggac
     3541 gggaactgat cgcctcgata aatcgacgga ccgcaggcga tcgtgaacgt ccggagccgt
     3601 ccgtctggac gcaattccgt tccacccagc gtgatccctg ggttcactcg gatcgtaaag
     3661 atctcgtggg ccatttcggc aaggttgacg ttcaagcctg aattggaatt ctgacgaccg
     3721 gtctctggcg ttagatgagg gttgcgtcga gtgtattctt cgggaatcaa atcggcgtgg
     3781 agcgatgagt tctcgtaatt atagaatagc cggccgtagt cgtcctgggt cacgccccac
     3841 tggccgcgac gggccatcgg ctcctgcacg agcttgccgt cgataaactt gtgccgaaat
     3901 gtgcaatcgg cgctgtacaa ccaattgtcg agagcgtgaa agaggccatt gtcagtgtgt
     3961 tgggggtttc cgggcctacc gtatgtgccg accaggcgct tctcgtcgca tttcaggtcg
     4021 ccgtcgatgt cgcggcagta ccagaggttg ggaggttcgg cgacgagcag cccgccagcg
     4081 acgaacgaca aggtccgcgg catcaccagg ttgtcgagga acacggttga acggtccatc
     4141 acgctgtcgc cgtttgtatc ttccagcaca acgacgttac ctataggagt tccttcgtcc
     4201 gagccttcca gatcgcgcat atacccgcgc atttcgacga cccagatgcg tccgtcggga
     4261 tcgaactcga acatcaccgg gttgacgacc aacggctcgg cggcgacaca ctcgatgcga
     4321 aaaccgggcg cgacctggaa cgacttaagc gcctcggccg gagtcaacgc cggggagggg
     4381 ggaatcttga tccgcttgaa aagttcatcc gccgaaaacg gatcgtacgg cgtcagcgat
     4441 tgctcacgat gcgtgatctg tcgttccttg gggaccgact gcgcatgcaa cggcgctgcc
     4501 gcaaaaactg cgacgatcaa accgcacgtc aacttagtca cgagcggttc tcgcatgatt
     4561 gggctcctgt tgcacggctg attgacgcgc cgattgacga tggagcggga gtaagattgc
     4621 gctttccgac gcgattctat cgtctccccg cgccttccgc gacttgatga tgcccaagcg
     4681 cttcgaagtc gaatcgactg aagcaactag atgccacgcc tgccagtcgg taatatcgtt
     4741 tacataagcc aagatcgaga cgtaacctac gacacacgtc gtggaggcca ccaaaaaaac
     4801 caagaggccg gatggccgac gcccgactgg gccatcgaac acccgatgga ggtttgtcag
     4861 ccgtagacag gcttttcgcg gcggacgcga ctcacgacag ccgcgacggc gaggccgatg
     4921 ccggcaagca cgaagctcga cggttctgga acgaccgaac caacaagctg gaccggcaaa
     4981 aaggtcgcac cgttgtcagg ggaatcgtcg ctaatcctcc aggtaccggc cgagccgata
     5041 gcgccgccat tggcggcgct agaacctggg gtcacctcga aacggatatc aaggctcgag
     5101 gtcacggtaa tcggggcgaa tgtcaaatca gtgtcgacga acgtaacgga cggcagattg
     5161 atggtagaaa gcttcgtgaa cgcgccgccg ttgacggaga cgtccacgtc gatgctaccg
     5221 gggcctgttc gttggccggg acgcccagcg cgatcagcac taacgacgtt ggctcaggca
     5281 ccgaagccac ccgcatgatg aggaacggcg ccggcgatgt cccgatggtc cgggtgggtc
     5341 gggacgcttt catggcctgc gtttgagagc gatacccggg attctaacct cctcgtaagg
     5401 acgggactgg agccggaatt ccttgatctg ttcggggggc tggtcgaact cgacaacgag
     5461 ctgccggaag tccttcacac cgttgcccga ccggaccgcc gccgggagta tcttgccgtc
     5521 gacgtcgacc gcgacgagcc gcactggctt gtcctggatg ttatgaatga ccgataacgc
     5581 cgccccctcc ttggtggcga ttgctccact gaagatgtaa ctcggaccga tcgcggagcc
     5641 gacagcgacc gagttctttc cctgggtcac gatggttttc cagggaccgg ccgcgacctt
     5701 gaaacggacc gtgccagtgc ctgcatcggc ctgaagtagg gcggccgttt cgctcagacc
     5761 gggtaagggt ttgccgtctc gcatggccgg ccccacggct gcgccgcggg cctcggcgat
     5821 cgaccactca tggtcggctc cgtcgggaat gcgggcaagg cgcaccagaa ccaacttaag
     5881 gacgccggtg tcgcccgaga ttcccggccg gatcggatca catggcgccg ggtgtaacgg
     5941 cgtcccgtcc ggacgccacc aggtatcggg gccggatggg aacgaggata ccccgatgat
     6001 ctcgatcaag gctccgctgg gcaggctccg gacccagcgg cgatcacccg cttgcttttc
     6061 gtttggtttt tcggctggtt gcgccgcggt ctgtggcggc cgcgctgggc gaatcccacc
     6121 gttcggcgcc ggctgcgaca gggcctgggc cgtcaggccg atggcgacga gggcgcctgg
     6181 cacgaccagc ttgagcttct cccacatcat catcttcaat actccttcgg taagatctgt
     6241 gactgccgcc gggaacgcgc ccttggcgca cgaagttgcc gctcggaccg tcgattggac
     6301 gagcttctga ggaatagccg cgcgggcttt gcgggtgaga gccgtggcca agccgactgc
     6361 gcctggcgcg atgcctcgcc gcgccagccg ctcccgcaat ctaagccggc ccttcgtcag
     6421 ccgactcttg actgtcgcca ccgggcagtt gagctgccgg gccgcctggt cgtaagtcat
     6481 accttggaga tggcacagct cgacggcgca ccggtacttg ccgggcaggc gatccagctc
     6541 ttcacccaca gccttgcgga ggtccttgcg ttccacttcg cccactggat catcggacga
     6601 gaccaccgga gctgagccca tgcaggatcg tcgtcgctcg gcctcggagc gggcgcgaag
     6661 ggcaatgcgg tgagcgactc catagagcca gcggccgagc gatccattga cccgcaccga
     6721 tccggcctta cgaacgagaa cgaggaatgt ggcttggaaa gcgtcctccg cctcgtaagc
     6781 gtcgccgagc acccggtggc agatccccca gaccatcggt ccatggcgat ccaccaaggc
     6841 ctcgaacgcc agttccgccg ctgtcgcggc ttcgaccgat tgggcgcgct tggccgtata
     6901 acgctcgaga agctcgctgt cgctcagccc cgttgccgtt cccgccgcga aaagggctcg
     6961 cagttgccgc aacgcggaag gaagttgtcg tctacccatg ttggtcgttc tcgccgagac
     7021 gttcgaacca gtcgtcacta ctatgcaagc tgtgacgtcg acggactgat ttttttcaag
     7081 aaattcggaa tagtcgcgcg gtcccgttag tctacctgtt agttagagag aatgaccggc
     7141 atgcggaggc gaagcccatg gccagcaatt ccagcgggcc ggctctccga acgatccgcg
     7201 cgttattcac cgcggggact atcggtgggc tgaccgacgg agaactcctc gagcgattca
     7261 ctgcccgcga cggtgatggc gccgaactgg ccttcgccag tttggtcgag cggcacggtc
     7321 ccatggtcct gcgtgtgtgc cggacgttac tgcgcgacgc ccacctagct gacgatgcct
     7381 gtcaagcgac gttcgtcatt ctggcgctca aagccggttc gatcagagga caacattccc
     7441 tgacttcgtg gctctatagc gtggcttaca acgtagcggc gactgcgcgg tcgtcgtcgg
     7501 ctcggcgtcg cgcccacgaa ttgaaggccg cacaggcaag gccgctcact tatatcgaag
     7561 atgcaccgga cgaactcggc ccagtgatcc atgaag
//
LOCUS       DATAQL010000456         4864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3863258, whole genome shotgun sequence.
ACCESSION   DATAQL010000456 DATAQL010000000
VERSION     DATAQL010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3863258"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..853
                     /locus_tag="VHS97_07865"
     CDS             383..853
                     /locus_tag="VHS97_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3448157.1"
                     /translation="MIVLKVNRKDFMVDVSPDTPLLWVIRETLGLTGTKFGCGMAQCG
                     ACTIHLEGEAVRSCVTPVSRAAGREVTTIEGLSTDLGHPLQQAWIAEDVPQCGYCQSG
                     QIMAAAVLLRENPSPDDAEIDDAMTGNICRCGTYQRIRRAIHLAAEIKRKGGGK"
     gene            850..3018
                     /locus_tag="VHS97_07870"
     CDS             850..3018
                     /locus_tag="VHS97_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3448158.1"
                     /translation="MSQIFNLSRRGFLKTTALAGGGLVLGVSLARVSPPAAAANQKPS
                     TFAPNAFVRIGTDDSITVLANHSEMGQGIYTGLAMMLVEELDADWSKVRVEAAPVDRA
                     YNNTLYGIQVTGGSTSTWTEWERVRMAGATARAMLISAAAATWSVDPASCRTEQGQVV
                     HPDSGRRLSFGRLAEKAAGLTPPQNVALKDPKNFKLIGKSTKRLDTPEKVNGTGLFGL
                     DVNVPGMLVALVARPPVFGGKVKSFDATKAKAVPGVRHVVEIDRGVAVVADGFWPARK
                     GRSVLEIVWDEGPLSELDSKTQHEHYVELAKKPGAVARKQGDVVEALSRSSKKLEAIY
                     DLPYLAHAPMEPLNCVADVRPESCEVWTGTQMQTFDRDAAAHAAGLKPEQVKVNTTLL
                     GGGFGRRAVLDGHFVREAVQLSKAVGKPVKVMWTREDDIRGGFYRPVAHHVIAAGLDA
                     TGNPAAWQHRIVCQSFIVGTMFEAVIVKDGVDETAVEGAADLPYEVPNVLVDWQQAPG
                     GVPTLWLRSVGHSHTAFVVETFLDELAHAAGKDPFEYRRALLQKQPRHKRVLEVVADK
                     AGWGKPLPAGRGRGVAVHQSFGSLVAQVAEVSLSDQGKLRVHRVVCAIDCGPVVNPDG
                     VRAQMEGGIVFGLTAALYGEITFEKGRVKQRNFHDYPMLRMNEMPEVEVHIVASTDRM
                     GGVGETAVPPIAPAVANAAFAVTGKRIRSLPIRAEDLKSV"
     gene            3053..3718
                     /locus_tag="VHS97_07875"
     CDS             3053..3718
                     /locus_tag="VHS97_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448159.1"
                     /translation="MPKTDSRPQRVDLAVKLTLAIAALSFVVLAQESDGTVNIAHAGS
                     SVAGTGGGTGAQAQKDDTASRAAFQAAYKVFMHPRCMNCHPAGDAPLQGDDSHIHAQN
                     VKRGPDGKGKYALKCANCHQLANLPGENMPPGSPNWHLPGPDMPLVFEGKSPRELARQ
                     FLDPKKNGGKTLEELIHHVADDKLVAGSWEPGDGRTKPPLSHSEFVRQFREWVEKGAA
                     VPE"
     gene            complement(3790..>4864)
                     /locus_tag="VHS97_07880"
     CDS             complement(3790..>4864)
                     /locus_tag="VHS97_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448160.1"
                     /translation="ADKIVVVCPSCQATLRVRRVYIGNPVRCKQCEHVFTVAPPLELD
                     LRPAEEVTRDNVESPARQAETEREIRRLNEELDRVTTERNDLGEKCDRLTIELNEIRA
                     SLGEVAPELVRPMAEERDSLRAQVLRLGDEIRDLLADQSAHVRVAAELEQHVEELNTA
                     HSELASLGAKLEERGVELDSARALHTGLSREHQTAIEEMKALQATLDERDQAIRLQAD
                     QHHAELESHVQAIDHARQLDREASERVQAELVALGARHGRLQEELNARDDLCTQLQAR
                     ILELEKDQERIESEYQSMIEAERRKQNELSEACAAPTAELQTALAQIKDLQEWLSESE
                     RLNRDMAALLENVGIVYSPPVRP"
BASE COUNT          913 a         1447 c         1574 g          930 t
ORIGIN      
        1 cctattgcgt gattctcgtg ctcaaacatc tgggcctggc caccaagatc gtcgagccca
       61 agagagtgcg acgctcccgg cgcaccgccg cggaataacg ctcccggcgg cgtttagtcg
      121 ctctcgttcg gcggttgtcc acaaccgctt gccggttcgt ccccggagcc gtcgtcgtcg
      181 cggccttcat tctcaatggc cgcgacgtcg atcgattttc gaaggctcgg atatggagca
      241 tttccgtttc aatccgcggc cgaacctggc tcagcaatga ctcggtctgg aagacgggcc
      301 aattgatcgg ttacgatccc cccaatggct ctgtgaaaga tgaaaatgga tatgccgcag
      361 cgacggtgag ggggtgatgg gcatgatagt gctcaaagtc aaccggaaag acttcatggt
      421 cgacgtcagt cccgatacgc cgctcttgtg ggtaatccgg gaaacgctgg gactgaccgg
      481 cacgaaattt ggctgcggca tggcgcaatg tggggcttgc acgatccatc tcgaggggga
      541 agccgtgcgg tcgtgtgtca cgccggtatc gcgtgcggcc ggcagagaag tcacgacgat
      601 tgagggcctc tcaaccgacc tgggtcatcc gttgcagcag gcctggattg ccgaggacgt
      661 gccgcaatgt ggttactgcc agtcgggaca aatcatggcc gcggcagtgt tgctgcgtga
      721 gaacccgagc ccggacgacg ccgaaatcga cgacgccatg accggcaaca tctgccggtg
      781 cgggacgtat cagcgcatcc gccgcgcgat tcatctggca gcggagatca agcgaaaagg
      841 gggtggcaaa tgagccagat cttcaactta agccggcgcg gctttttgaa gacgaccgcc
      901 ttggctggtg ggggcctcgt tttgggcgtc tcacttgcca gagtgtcccc tcctgcggca
      961 gcggccaacc aaaagccctc gaccttcgcc cccaacgcgt tcgtccggat cgggacggac
     1021 gattcgatca cagttcttgc caatcactcc gagatgggcc agggcatcta tacgggcctg
     1081 gcaatgatgc ttgtcgaaga gctcgatgcc gactggagca aggtccgggt cgaagccgca
     1141 cccgtcgatc gggcttacaa caacacgctc tacggtatac aggtaaccgg cggcagcacg
     1201 agcacctgga cggagtggga gcgggtccgc atggccggcg cgacagcacg cgcaatgctg
     1261 atatcggccg cggcggcgac gtggagcgtc gatccggcga gttgccggac cgagcagggg
     1321 caagtcgtac atccggactc tgggcggcgg ctgtctttcg ggcggctcgc ggaaaaagcg
     1381 gccggcctga cgccaccgca aaatgttgcg ctcaaggatc cgaagaattt caagctcata
     1441 ggcaaatcca ccaaacggct cgacacgccc gaaaaggtga acggcacggg actgttcggt
     1501 cttgatgtca acgtcccggg catgctggtt gccctcgtcg cgcggccgcc cgtttttggc
     1561 ggcaaggtga agagctttga cgcgaccaaa gccaaggctg tgccgggtgt ccgccacgtg
     1621 gtcgaaatcg atcgcggcgt tgcggtggtg gccgatgggt tctggccggc gaggaagggt
     1681 cggtcggtcc tcgagattgt ctgggacgaa ggcccgctct cggaactcga tagcaagacg
     1741 cagcacgaac actatgtgga gctggctaag aagccggggg ctgtcgcacg gaagcaaggg
     1801 gacgttgtcg aagccttgag ccggagctcg aagaagctcg aggccatcta cgatttgccg
     1861 taccttgccc acgcgccgat ggagcctttg aattgtgtcg ccgacgtccg tcccgagagt
     1921 tgcgaggtct ggaccggcac gcagatgcag acctttgacc gcgacgccgc ggcccacgcc
     1981 gccggtctca aacccgagca ggtcaaggtg aacacgacgc tgctgggagg gggctttggc
     2041 aggcgtgccg tcctcgacgg ccacttcgtg cgggaagcgg tgcaattatc gaaggcagtg
     2101 ggaaagccgg tcaaggtgat gtggacgcgt gaggatgaca ttcggggtgg gttttaccgc
     2161 cctgtggccc atcacgtgat cgccgcgggt ctggacgcca ctggtaatcc cgcggcctgg
     2221 caacatcgaa tcgtttgtca gtcgttcatc gtcgggacca tgttcgaggc agtcatcgtc
     2281 aaggacggag tggacgagac ggccgtcgaa ggggcggccg atctgcctta cgaagtccct
     2341 aacgtgctgg tggactggca gcaagcgccc gggggtgtcc caacgctctg gttgcggtcc
     2401 gtgggacatt cgcacaccgc cttcgtcgtc gaaacgtttc tcgacgaact ggcgcacgcc
     2461 gctggcaagg atccatttga gtaccgccgg gcgttactcc aaaagcaacc gcgacacaag
     2521 cgcgttctcg aagttgtcgc cgacaaggcc ggctggggaa aaccgctgcc agcggggcgc
     2581 ggacggggcg tcgccgtgca ccaatcgttc ggcagccttg tcgcccaggt ggccgaggtc
     2641 tcattgtccg accagggtaa gctcagggtc catcgcgttg tgtgcgcgat cgattgcggg
     2701 cccgtcgtga atcccgatgg cgtccgtgct cagatggagg gaggaatcgt cttcggcttg
     2761 accgccgcgc tctacgggga gatcacgttt gaaaaaggac gcgtgaaaca gcggaatttt
     2821 catgactacc cgatgctgcg gatgaacgag atgcccgaag tggaagtgca cattgtcgcc
     2881 agcaccgacc ggatgggggg cgtgggcgaa accgcggtcc ctccgatcgc tccggcggtg
     2941 gccaacgcgg ccttcgcagt cactggcaaa cgcatccgaa gcctgcccat ccgcgcggag
     3001 gacctgaaaa gtgtttgatt ttccgccatc gcgcgtcgaa gagagacaat ccgtgcctaa
     3061 gaccgatagc cggccgcagc gcgtggatct cgccgtgaag ctgactcttg ccatcgctgc
     3121 gctctctttc gtggtgttgg cacaagagtc ggacgggact gtcaacatcg cacatgccgg
     3181 cagcagtgtc gccggcacgg gcggagggac cggggcgcag gcgcagaaag atgacaccgc
     3241 gtcgcgcgcg gcattccagg cggcttacaa ggtgttcatg cacccgcgct gtatgaactg
     3301 tcatccagcc ggcgatgcgc cgctgcaagg cgatgacagc catatccatg cgcagaacgt
     3361 caagcgcggg ccggacggca agggtaagta tgctctcaag tgtgctaact gccatcagct
     3421 tgcgaattta ccgggcgaga acatgccacc gggcagcccc aactggcact taccgggtcc
     3481 cgatatgcct ctcgtgtttg aggggaaaag tccgcgagag ctcgctcgtc agttcctcga
     3541 tcccaagaaa aacggcggca agacattgga ggaattgatc caccacgtcg cggacgacaa
     3601 gttggtggcc ggctcctggg agccgggcga cggccgaacg aagcctccgc ttagccactc
     3661 cgaatttgta cgacaattcc gtgaatgggt ggagaagggg gcggccgtcc cggaatgatc
     3721 agcgatcctc gatggttgcg aacgccctca cgccgaaggc ggcaggacgg atccatattc
     3781 aattacactt caaggtctga ctggcgggga gtatacgata ccgacattct cgagcagtgc
     3841 ggccatgtca cggttgaggc gctcggattc gctaagccac tcctgcaagt ccttgatctg
     3901 ggcgagagcg gtttggagct cggcggtcgg cgcagcgcac gcctcgctga gctcattctg
     3961 tttccgccgc tcggcttcga tcatcgactg atattcggat tcgattctct cttggtcctt
     4021 ttcgagctcc aggatgcggg cctggagctg cgtgcataag tcgtctctcg cattcagctc
     4081 ttcctgaagc cggccgtgcc gcgcgcccag agcgacaagt tcggcttgga cccgctcgct
     4141 cgcttcgcga tcaagctgcc gcgcatggtc gatcgcttgg acgtgactct cgagctcggc
     4201 gtgatgctgg tcagcctgga gtcggatcgc ctgatcgcgc tcgtcgagtg tcgcctggag
     4261 cgccttcatc tcttcgatcg ccgtctgatg ttcgcgactc aagccggtgt gcagagcgcg
     4321 cgccgaatcg agctcgacgc cgcgctcttc aagcttggcg cccagcgatg ctagctcgct
     4381 gtgggcagtg ttcagttcct cgacatgttg ttccaactca gcggcgacgc gtacgtgcgc
     4441 cgattgatcg gccagcaggt cgcggatttc atcgcccaga cgaagaactt gggcccgaag
     4501 tgaatcgcgc tcctccgcca ttgggcgaac cagctccggt gcgacctcac ccaggctggc
     4561 gcggatctcg ttcaattcta tcgtgagccg atcgcacttt tcgccgaggt cgttccgctc
     4621 ggtcgtgacc cggtcgagct cctcgttcaa tcggcggatc tcgcgctcgg tctcggcttg
     4681 cctggccggc gattccacgt tgtcgcgtgt gacttcctca gcaggtcgca aatcaagttc
     4741 gaggggtggc gccaccgtga agacatgctc gcactgcttg caacggaccg ggttgccgat
     4801 gtatacccgg cggactctca aggtcgcttg gcagctcggg cagaccacaa cgattttatc
     4861 ggct
//
LOCUS       DATAQL010000457         6838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5390114, whole genome shotgun sequence.
ACCESSION   DATAQL010000457 DATAQL010000000
VERSION     DATAQL010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5390114"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VHS97_07885"
     CDS             complement(<1..613)
                     /locus_tag="VHS97_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448161.1"
                     /translation="MKNFVRLVQFAWRYKIRFGLSVCCAVMVALLFFTELGAVYPLLH
                     ILFGSQNPQRWISEKIVGLETDILILQAREEEARQVLTDAQSSEPHNDELRQRFQRVH
                     ENFERTEADLRTHERRFGVGGPKENADPFGGREAIQADEAKLRRDHRVAEARWNELRE
                     CVDFFTKRKVRSLTIRQREIQQEISDQEKMLNRCKLAQPFVIKY"
     gene            880..2001
                     /locus_tag="VHS97_07890"
     CDS             880..2001
                     /locus_tag="VHS97_07890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3448162.1"
                     /translation="MKLLALVESTDHVCCRYRIRAFEPALNEAGWSVTYEGFDQGSLL
                     RPFQLRRAARFDSVILQRKLLPGWQLRALRRSSRHLVYDFDDAVLFRDSYDRRGHESG
                     WRSRRFSRTVCMVDTVVAGNDFLADCALRAGARVDRVHVIPTCVDPRVYAIARQESEA
                     SRLDLVWIGSAGTLKGLEQSRPIWERLRQAFPQVRLRVICDRFPERFPIPMVPVPWNE
                     ETETREIAAGHVGVSWLPDDLWSRGKCGLKILQYQAAGLPVIANPVGPHSEIIKAGET
                     GFLATSAEEWVAALSELADSPRIRQKMGQRARNRVESDYSTSAWAETFVTSMTGTCPR
                     APRTSWKVDHSRPTSVGTGFEPHGAKLKTRRALNQIGDR"
     gene            2046..3821
                     /locus_tag="VHS97_07895"
     CDS             2046..3821
                     /locus_tag="VHS97_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX3448163.1"
                     /translation="MASRLREIQASDGIRRMFKPPEWEWSQFSDVGWWSRDGWGEVLL
                     GKAGLRLDEWREQGNLAIIKSGPHRVVYRVDLPQGSIYVKHFLVPDWRAALRQWVRRG
                     KGRNEGKRSQQLASIGVPTITPIALGERRKRKFLLENYLVTLAISSAIPLDEFVERDL
                     PEQPEPLRSQIRQRLAQAMAVLTARLHDAGLYHQDFHPGNIMVRLAADQSPELFMIDL
                     DALRTSRRVTWKLARQNLALLDHYFWLRSSRTDRYRFLKTYLQNRAEEAPDVRRFALR
                     IEDATRLWAERLWTRWGRRCRSSNKYFEVYQNSTCWCVASRDLDPTLVRSLLENPDLP
                     FQNPATRILKNSRTTTVAETTMLARGALTRVVYKRFNRKKWLDPLLNLFRPSRAWRSW
                     QAGQHLVSRGIPTPQNLAFLSRTRSFATDRIFWFLPHETYLITVKEDHVVDLATYARK
                     VLSNLPPEVRRARILGLTAALAQLVRSLHERSLSHRDLKASNILINVNSIDRGEQLSL
                     IDLVGVRLRHPLPWSRRIQNLARLCISLDAVAGRTRSDSLRFLRLYLPWGLSPLNDWK
                     RIWRSIEKGIGHKRSRNRRRGRPLS"
     gene            3841..4791
                     /locus_tag="VHS97_07900"
     CDS             3841..4791
                     /locus_tag="VHS97_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448164.1"
                     /translation="MTGGMDHQPIGRRRLFFALGAIALLLSSTSCATLSDRGKPLVPT
                     RYQLRSGPFIIFSNFAMADDPPALRCLQALERDMKQHLDFAPRMDAEPVEIYVLDDRN
                     AFAHFLKFYYPELPPRRAFFLAQGSQRVVYTYSSPRLEEDLRHEATHALLHGSFGNLA
                     LWLDEGLAEYFETDLAQPGAERERIDHITEDLAGGWSPSLPRLESLNDIRQMTPRDYR
                     EAWAWVHLFLDNSRADKEVLLASLASLNVAPDKLRLEEKGATNERLLAHIKSLQSRAS
                     ATPPIEADHSVRMQNKTAEVSAANPATRGLLRRFWTWIGL"
     gene            4919..6697
                     /locus_tag="VHS97_07905"
     CDS             4919..6697
                     /locus_tag="VHS97_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3448165.1"
                     /translation="MKWLTAVAKLLAVSLAALGLLSGPDAARANEGVEGNWQGQLIVT
                     PQITLRITLEINKGTNGSLSGNWGSPDEALSALPLESIVLKGAVLTFRAKPTGATFKG
                     KLNETGTEVVGDWTQNGKNLAITFKRFDPSTVAVALIPQELEGIWEGKLKFNAGIELR
                     IALKVEKGKDGALKATLASPDQGANSIPISSIGLKDDLLTLESKFIAAKFTGKRNKEK
                     SAFEGEFNQGGVKLPLTLKKTDKIAEAARPQTPKPPFPYRSEEVVYENKVGGIKLAGT
                     LTLPAGKGPTPAVILITGSGAQDRDESILGHKPFHVLADYLTRRGVAVLRVDDRGVGG
                     STGSTTTSTSDDFAGDALAGLDFLKRRTDIDSKKIGLIGHSEGGLIATIAAGRSQDVA
                     YIVLMAGTGLPGSDILETQGQLILKAGGASESEMKKERGYQKRLIDIIATEKDEQTAD
                     AKLMAAWKEIVAAMPEAERKALDATGGSLHEAAIKGFNNAWFRYFLKYDPRPTLRTVR
                     CPVLAINGEKDLQVSAKENLAEIAKALQSGGNSNVKTVEFPGLNHLFQPCKTGSPSEY
                     ASIEMTIAPEALKTIGDWIALKTGNH"
BASE COUNT         1403 a         2039 c         2060 g         1336 t
ORIGIN      
        1 gatacttgat gacaaatggc tgggcaagct tgcaccgatt cagcattttt tcttggtcgg
       61 agatttcttg ctggatctcg cgctggcgga tcgtgagcga acggactttc ctctttgtga
      121 aaaagtccac gcattcccgc agctcgttcc accgcgcctc cgccacgcgg tggtcgcgac
      181 gcagctttgc ctcatccgct tggattgcct cgcggccgcc gaatggatcg gcattctctt
      241 tcgggccgcc taccccgaag cgacgttcgt gtgtgcgcag gtcggcctcg gtacgctcaa
      301 agttttcatg gacccgctga aatcgttgcc ggagctcgtc gttgtgaggc tcactgctct
      361 gggcgtcggt cagaacttgc ctggcttctt cctctcgcgc ctggagaatg aggatgtcgg
      421 tctcgaggcc gacgatcttc tccgaaatcc agcgctgggg gttctggctg ccaaaaagga
      481 tatggagcag tggataaacc gctcccagct cggtgaagaa aagtaaggcc accatcaccg
      541 cgcagcagac ggaaaggccg aaccgtatct tgtagcgcca ggcaaactgc acaaggcgaa
      601 cgaagttttt cataagcgag agaggccgca gacgggaaga ccctaagtgc gtccatgcat
      661 cattggagcc cgcttgtgag cgccaatgtt cgggcggcgc cgtgtgtacc atgattggag
      721 cacagcggca atccaacttg aattcgatcg gcgacggaca tgcccgctgg tcagtcggtc
      781 ttggagattt agagcggaac ggttatgatc gcgacgattg acgagacggg cggcacgccc
      841 tggccgtcga gctgaccgcg caccgtcaag gaggacggga tgaaactact tgccctggtt
      901 gaatcgactg atcacgtttg ctgtcgctac cggatcaggg cgtttgaacc tgccctcaat
      961 gaagcgggct ggtcagtaac atacgaagga ttcgatcagg ggtcgctcct tcgaccgttt
     1021 cagcttcgcc gggctgcgcg gttcgactcg gtaatactgc agcgcaagct gctcccagga
     1081 tggcaattgc gcgctttgcg ccggagctcg cggcatctcg tgtacgactt cgacgatgcg
     1141 gtgctctttc gcgattctta cgatcgtcgc ggtcacgaga gcggctggcg ttccagacgg
     1201 ttttcgcgga ctgtctgcat ggtcgacaca gtcgtcgcgg gtaacgattt cctggccgac
     1261 tgcgcgctac gggccggggc cagggtggat cgcgtgcacg tgatccccac ttgcgtggat
     1321 ccacgggtct atgccattgc ccggcaagag agcgaggcga gccgacttga cctcgtctgg
     1381 atcggttcgg cgggcacgct caaagggctg gaacaatcgc gtccgatctg ggaacggttg
     1441 cggcaggcat tcccccaggt gaggttgcgt gtcatctgtg accgctttcc cgagcgtttt
     1501 cccattccga tggtgcctgt cccctggaac gaagagaccg agacgcgcga aatcgcggcc
     1561 ggacacgtcg gggtgagctg gctccccgat gatttgtgga gtcggggtaa gtgtgggctg
     1621 aagattcttc aatatcaggc ggccggtctg cctgtaatcg ccaatccggt cggcccgcat
     1681 agcgagatca tcaaggccgg cgaaacgggt tttttggcga catcggccga agagtgggtc
     1741 gccgctctct ccgagctggc agatagtccc aggattcggc agaaaatggg ccagcgagcg
     1801 cgcaatcgcg tcgagtcgga ttactcgact tctgcctggg ccgagacctt cgtgacctcc
     1861 atgaccggga cgtgccctag ggcacctcgc acgtcgtgga aagtcgacca ctcgcggccg
     1921 accagcgtgg gcacgggatt tgaaccgcac ggcgcaaagc tgaagacacg acgtgccctc
     1981 aaccagatcg gcgatcgatg accctctgca ccccgcacga acgatcgacg gtatcagggg
     2041 ctcgcatggc gtcaaggctg cgggaaatcc aggcgtccga cggcatccgg cggatgttca
     2101 agccgccgga atgggaatgg tcgcaattca gcgacgtcgg atggtggtcg cgggacggtt
     2161 ggggcgaggt acttctcggc aaggctggat tgcgcctgga cgagtggcgc gaacagggga
     2221 acctcgcgat catcaaatcg gggcctcatc gcgtggtgta tcgcgtcgac cttccccagg
     2281 gcagcattta cgtcaagcat ttcctcgtac cggactggcg ggccgcgctc cgccagtggg
     2341 tgcgtcgggg caagggtcga aacgaaggta agcgctccca gcaactggcc tcgatcggag
     2401 tgccaacgat cacgccgatc gcgctgggcg aacggcggaa acgcaagttt ctgctcgaga
     2461 attatctggt gacattggcg atttcatccg cgattccgct cgacgagttt gtcgagcgag
     2521 atcttcccga gcagcccgag ccgcttcgct ctcagatccg ccaaagactc gctcaggcaa
     2581 tggccgtttt gacggcccga ctgcacgacg cgggtctcta ccatcaagac tttcatcccg
     2641 gcaacatcat ggttcggctc gcagccgatc aatcccctga actcttcatg atcgatctcg
     2701 acgccctgcg cacgagccgc cgggtaacct ggaaactggc ccgacaaaac ctggcgctcc
     2761 ttgatcacta cttttggctg cgaagcagcc gaaccgatcg ttatcgcttt ttgaaaacct
     2821 atttgcaaaa ccgtgcggaa gaggctcccg acgtccgccg gttcgcactc aggatcgagg
     2881 acgcgacgcg tctctgggcc gaacggctat ggacacgatg gggacggcgg tgcaggtcat
     2941 caaacaagta ttttgaggtt tatcagaaca gcacatgctg gtgcgtggcg tcgcgcgatc
     3001 tcgaccccac tcttgttcgc tcgctcttgg agaatcccga cctcccgttc cagaatcccg
     3061 cgacacgaat cttgaagaat tcgcgcacga ccaccgtggc cgagacgacg atgctggcgc
     3121 gaggcgctct gacccgagtg gtttacaagc ggttcaatcg caagaagtgg ctcgatccgc
     3181 tcttaaacct ctttcgtccg tcgcgtgcgt ggcgatcgtg gcaagcaggc cagcatctcg
     3241 ttagccgcgg catccccacg ccacaaaacc tggcatttct ctcgcgcacg cgctcgttcg
     3301 cgactgaccg gatcttttgg tttctaccgc atgagaccta cctgatcacc gtcaaggaag
     3361 atcacgtggt cgacctcgcg acctatgccc gaaaagtgct gtccaacctc ccgccagagg
     3421 tccgccgcgc tcggattctt ggcttgacgg ctgcgcttgc acagctagtt cgatccctcc
     3481 acgagcgttc gctctctcac cgcgatctga aagcctcaaa catattgatc aacgtcaatt
     3541 cgattgaccg gggggaacag ctgagcctga ttgacttggt cggtgtacga ttgcgtcatc
     3601 cgctcccgtg gagccggcgc atccaaaacc tggcgcggct gtgcatcagt ctcgacgccg
     3661 tcgcggggag aactcggtct gactcgctgc ggttccttcg cctttacctt ccctggggcc
     3721 tctccccctt gaacgactgg aaaagaatct ggcgatcgat cgagaagggg atagggcaca
     3781 aacgatctcg caaccggcgg cgagggcgcc cgctttccta aagcagatgg gagtcgagta
     3841 gtgacgggtg gtatggacca tcagccgatt gggcggcgcc gtctgttttt cgccttggga
     3901 gcgattgcgc ttttgctctc atcgacgagc tgcgccaccc tctctgatcg gggcaagcca
     3961 ctagttccga cacgctacca gcttcggagt gggccattca tcatattctc taattttgct
     4021 atggccgacg atccgccggc cctgcgctgt ctgcaggcgc tcgagcgcga catgaaacaa
     4081 caccttgact tcgcaccgcg catggacgca gagccggtcg agatttacgt cctcgacgat
     4141 cgcaatgcgt ttgcccattt cctcaagttc tattatccag agctaccgcc gcgtcgggcg
     4201 ttctttctgg cgcagggcag ccagcgcgtc gtttacactt actcgagccc tcgtctcgag
     4261 gaagacctgc gccatgaggc gacacacgcg ctgctccatg ggtccttcgg caatctggcg
     4321 ctctggctcg atgaagggct tgccgaatat tttgagaccg atctggctca acccggggcc
     4381 gaacgtgagc gcattgatca tatcaccgag gacctcgcag ggggctggtc tcccagcctg
     4441 ccgcggctcg aatcgctgaa tgatatccgc cagatgaccc cgcgcgacta tcgtgaggca
     4501 tgggcatggg tccatctctt cctggacaac tcccgggcgg ataaggaggt cttgctcgcg
     4561 tcgctggcct cgctcaatgt ggcgcctgac aagctgcggc tcgaggaaaa aggagcgacc
     4621 aacgaacgcc tcctcgccca catcaaatcc ctgcagtcgc gcgcaagcgc cactccaccc
     4681 atcgaggcag atcactccgt ccgcatgcag aacaagaccg ccgaagtatc cgccgccaat
     4741 cccgcgacgc gcggcttgtt gcggcgattc tggacgtgga tcgggctctg aacatcgttt
     4801 gactgagacg gccccgctcg gttgacagaa acggccccgc tcggttattt agaagatcgt
     4861 ctaacaaatc gatatcgcat cagtttcgag ggagccggtc gcagccgggg ttagggccat
     4921 gaaatggctg acagcagtcg caaagctctt ggcggtgagc ttggccgcgc tgggattgct
     4981 atccgggccc gacgcagccc gcgccaacga aggggtcgaa gggaactggc aagggcagct
     5041 catcgtgacg ccccagatca cgttgcgcat cacacttgaa ataaacaagg ggacgaatgg
     5101 atcgctttcg ggcaattggg gcagtcccga cgaagcgctc agcgcgctgc cactcgagtc
     5161 gatcgtgctt aagggcgccg tcctcacgtt tagagccaag cccacagggg cgacgttcaa
     5221 gggaaagctc aatgagactg ggacagaggt cgtcggcgac tggacgcaga acggcaagaa
     5281 cttggcgatt actttcaaac ggttcgatcc gtccacggtc gctgtcgcgc tgattcctca
     5341 ggagctggag ggaatctggg agggcaagct caagtttaac gctggcatag agctccgcat
     5401 tgcgctcaag gtagagaagg gaaaggacgg cgccttgaaa gcgactctcg ccagccccga
     5461 tcaaggggcg aacagcattc ctatcagctc gatcggattg aaagatgacc ttctgaccct
     5521 cgaaagcaag ttcattgcgg ccaagttcac cggcaagaga aacaaggaaa agtcggcatt
     5581 cgagggcgag ttcaaccaag gcggcgtcaa gttaccgctc acgctcaaaa agaccgacaa
     5641 gatcgccgag gccgcgcgac ctcaaacgcc aaaaccgccg tttccctacc gctcggaaga
     5701 agtcgtctat gagaataaag tgggcggaat caagctcgcg ggcactctga ccttacccgc
     5761 ggggaaaggt ccgactccgg ccgtaatctt gatcacgggc tcgggcgctc aggatcgcga
     5821 cgaaagcatt ctcggccaca agccgttcca cgttctggcg gattatctca cccggcgcgg
     5881 ggtcgcagtc ttgcgggtcg acgaccgtgg agtcggcggt tccaccggct cgaccacgac
     5941 gtcgaccagc gacgacttcg ccggagacgc actggccgga ctggatttct tgaagagacg
     6001 aacggacatc gactccaaga agattggact gatcggccat agcgaaggcg ggctgattgc
     6061 cacgatcgcg gcgggccgct cgcaggacgt ggcgtacatc gttctcatgg ccggcaccgg
     6121 actgcccggt tccgatattc tcgagaccca ggggcagctc attctcaagg ccggcggcgc
     6181 atcggaatcg gagatgaaaa aggagcgcgg ttaccagaaa cggctgattg acatcatcgc
     6241 cactgagaag gacgagcaaa ccgctgacgc gaagcttatg gcggcgtgga aagagatcgt
     6301 cgcggctatg cccgaggcgg aaaggaaggc gctcgacgcg actggcggtt cactccacga
     6361 ggccgcgatc aaggggttta acaacgcctg gtttcgttac tttctcaagt acgacccgcg
     6421 tcccacgctc cgcacggtcc ggtgcccggt cctggcgatc aatggtgaga aagacttgca
     6481 agtctcggcg aaggaaaacc tcgcagagat cgccaaggct ctccaatcag gtggaaacag
     6541 caacgtaaaa acggtcgagt ttcctggcct gaatcatctc tttcagccat gcaaaactgg
     6601 ttcaccgagc gagtacgcct cgatcgagat gacgattgct cccgaagccc tcaaaacgat
     6661 cggagattgg atcgcgctca agaccggcaa ccactagtca caatgaccag gtcgcgccgc
     6721 ttcataccaa aagctcgagc ggctcgcgag tgacggctgc cttgtcgtga acggccggca
     6781 agagcgggtg attcttcccg acggagtcac ggcttcgcca ccacaggagc aataccat
//
LOCUS       DATAQL010000458         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_808131, whole genome shotgun sequence.
ACCESSION   DATAQL010000458 DATAQL010000000
VERSION     DATAQL010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808131"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VHS97_07910"
     CDS             <1..501
                     /locus_tag="VHS97_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEX3448166.1"
                     /translation="DVTDRAGPVFQVPLLGRGLAVGDLDNDGRVDLLILPQNQPLVYY
                     HNQSEGGRSLTLLLEGTSSNRDAVGARVVVEFGGRRQYAWRTGGGSYQSASEARIHLG
                     LGLGTVDRVDSVEVTWPSGRVDRYTGLQAGRGYRLREGDPSSKTLPGFGHGNEGVANR
                     RLLGND"
     gene            652..>2661
                     /locus_tag="VHS97_07915"
     CDS             652..>2661
                     /locus_tag="VHS97_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX3448167.1"
                     /translation="MAHFRFQRDIRDHADSSRVETWLALWIGLAGLFMMVSGAWSRAA
                     ADETKPKNASGTPFSPEAIDFFEARVRPILVNRCIKCHGPEKQSSNLRLDSREAVLQG
                     GDSGPSLVPAKPEESLLIQAVAQTHEELKMPPGGKLPEPSVAIITQWVALGAPWSATP
                     SKGAASSRIEGHDPRAPHWSLQPLVSPSVPPVKDRNWVGTAVDAFILARLEAAGLTPS
                     ARADKRTLIRRATIDLWGIPPSAEEIDAFELDSTPDAFARVVDRLLASPRYGERWGRH
                     WLDVARYADTKGYVFVQDRNYPYAYTYRDYVIRAFNSDLPYDQFIVQQLAADQLPRGD
                     DARMLAAMGFLTVGRRFLLDQNEIIDDRIDVVSRGLLGLTVTCARCHDHKFDPIPSED
                     YYSLYGVFASSIEPAELPLLQKVEPGPGFADYERKLNAAKKARDDFLAERRDEVQGDL
                     TARFSVYLKAAYDLDLDGRSVRLDERARADKLDPRRLRGLIGLWKRYLEATPKSADPV
                     LGVWRAFTALPQKEFAAKAKDLHRDLTEPKDPTAPPIHPVVARAVLGSPPTSKGDVVA
                     RYVALFSELEARLKDQKTRSAGTAGSSLPEPEWESLRQALFGPKGPLLISVERARMFL
                     DQSQRGRLDKLNGAIEKVNATHPAAPARAMVMNDAQELYDPHVFLR"
BASE COUNT          511 a          800 c          829 g          521 t
ORIGIN      
        1 gacgtaaccg atcgtgccgg acctgtcttt caggttccgc tgctgggtcg cggtttggcc
       61 gtgggagatc tggacaacga tggacgggtc gatctgttga tacttccaca gaaccaaccc
      121 ctggtttact accacaatca atccgaaggc gggcgttcac tcaccctact tctcgaaggc
      181 acgtcctcga atcgagatgc cgtcggggct cgtgtcgtcg ttgagtttgg tgggcgtcgc
      241 cagtatgcat ggcgaacggg cggcggaagc taccaatccg cctcagaggc tcggatccac
      301 ctcggactcg gactcggaac ggttgatcgc gttgactcgg ttgaagtgac gtggccatcc
      361 ggccgggtcg accgatacac cggactgcag gccggacgag gctaccggct tcgtgaagga
      421 gacccatcct ccaagactct gccaggcttt ggtcatggaa acgaaggggt tgcgaaccga
      481 cgcttgctgg gtaatgatta aacaatcctc gaccatccat gctttgccgg ccggcttttc
      541 acgccttgga tctcgtttgg gattttgcga gacctcgaaa aagtcgcgtt cagcctcccg
      601 aacgccattc gactgtcgcg cgagcccagc catcgatggg atcataatga tatggctcac
      661 tttcgctttc agcgtgacat ccgcgaccat gcggattcga gccgagttga gacctggctg
      721 gctctttgga ttgggctcgc gggattgttc atgatggtga gtggggcgtg gtctcgggct
      781 gcggccgacg aaacgaaacc gaagaatgca agcggcacgc cgttttcgcc cgaagcgatc
      841 gacttcttcg aagcccgggt ccgcccgatc ctggtcaatc gctgcatcaa gtgccatggg
      901 ccggaaaagc aatcttcgaa cctgcggttg gacagccgag aggcagtcct ccagggcggg
      961 gattccgggc cgtctctcgt gccggcgaaa cctgaggaaa gcctgctcat ccaggcggtt
     1021 gcccagactc acgaagagtt gaagatgccg cccggcggca aattacccga gccttcggtc
     1081 gcgatcatca cgcaatgggt cgctctggga gcaccgtggt cggctacccc gagcaaggga
     1141 gccgcttcga gccgaatcga gggccacgat ccaagagcgc cgcattggtc actccagccc
     1201 ctggtgtctc cgtctgttcc gccagtcaag gatcggaact gggtggggac tgccgtcgat
     1261 gcgttcattt tggctcggct cgaggccgcc gggttgacgc catcggcccg ggccgataag
     1321 cggaccttga tccgccgcgc cacgatcgac ctttggggaa ttccgccctc ggccgaggaa
     1381 attgacgcat tcgagctcga ctcgacgccc gacgcgttcg cccgcgttgt cgatcgcctc
     1441 ttggcctcgc cacgctatgg agaaaggtgg ggccggcact ggctggacgt cgctcgctat
     1501 gccgacacca agggctacgt ctttgttcag gaccgtaact atccttacgc ctacacctac
     1561 cgcgattacg tgatcagggc gttcaacagc gatcttcctt acgaccagtt catcgtccag
     1621 caactcgcgg ctgatcaact gcctcgcggc gatgacgcaa ggatgttggc ggctatgggg
     1681 ttcttgacgg tcggccggcg gtttctcctc gatcagaacg agatcatcga tgatcggatc
     1741 gacgtagtta gccggggcct cctgggattg acagtcacgt gtgcccgctg ccacgaccat
     1801 aaattcgacc cgatcccgag tgaggactac tattcgttgt acggcgtatt cgccagctct
     1861 atcgagccgg ccgagttacc cctgctgcaa aaagttgagc ccggcccggg tttcgccgac
     1921 tacgagcgaa aactgaacgc ggccaagaaa gcgcgggacg atttcctggc agagcggcga
     1981 gacgaggttc agggcgacct taccgcgcgg ttctccgttt acctcaaggc ggcatacgat
     2041 ctggacctcg atgggcgtag cgtccggctg gacgaacgag cccgcgccga caaactcgac
     2101 ccgagacggc tccgaggcct gatcgggctc tggaagcgct atctggaggc gactccgaag
     2161 tcggccgatc cggtcctggg cgtctggcgc gccttcaccg ccttaccgca aaaggagttc
     2221 gccgcaaagg caaaggacct tcacagggat ctgaccgaac caaaggatcc gacagcaccg
     2281 ccgatacatc cggtggttgc tcgagcagta cttgggagtc cgccgacgag caagggcgat
     2341 gtggtcgcgc ggtacgtcgc gcttttcagc gagctcgaag cgcgcttgaa ggatcaaaag
     2401 acgcgttcag cgggtacggc cggcagcagt ttgcccgagc cggaatggga gtcgctccgt
     2461 caggcacttt tcggcccgaa gggtccgttg ttgatctcgg tggagagggc gcggatgttc
     2521 ctggaccaaa gccagcgggg gcggctcgac aagttaaacg gtgcgatcga gaaagtgaat
     2581 gcgacccatc cggcggcccc tgcccgggcc atggtcatga atgacgccca agagctttac
     2641 gaccctcacg tgttcctgcg c
//
LOCUS       DATAQL010000459         6282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3863810, whole genome shotgun sequence.
ACCESSION   DATAQL010000459 DATAQL010000000
VERSION     DATAQL010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3863810"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..723
                     /locus_tag="VHS97_07920"
     CDS             241..723
                     /locus_tag="VHS97_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448168.1"
                     /translation="MLRKLIIKPHSAAPDATTGEIDIAAVATVLVTSEVPDHPIDRAF
                     DPHRGPGGTRWIAGEPGEQSVILAFDAPQTIRHILLEIEEPEVARTQELQLSLSCDGG
                     RTYRELLRQEYNFSPAGTTFEREDWAVTAESVTHLRLVIKPDNGGKPCWATLTSLALR
                     "
     gene            997..1332
                     /locus_tag="VHS97_07925"
     CDS             997..1332
                     /locus_tag="VHS97_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX3448169.1"
                     /translation="MGKKLYVGNLTYNVNESDLEALFTPFGSVQSAQVIVDRDTNRSK
                     GFGFVEMGSDAEAQAAIQGLNGHDYDGRNLTVNEAKPREERGGGGRGGYGGGGGGGGG
                     YGGGGGRRY"
     gene            complement(1400..1675)
                     /locus_tag="VHS97_07930"
     CDS             complement(1400..1675)
                     /locus_tag="VHS97_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448170.1"
                     /translation="MFKLNSRDVAWDKLNHVGELRGWVDGSVIIERSDVLFRSIDFPK
                     MQFNQFMMLPYFHDGVPHDQTLWIDELAVGAKRISATLGPERTPDER"
     gene            complement(1913..2218)
                     /locus_tag="VHS97_07935"
     CDS             complement(1913..2218)
                     /locus_tag="VHS97_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3448171.1"
                     /translation="MIPDGWYEMFSPESHPNLVPADRWPQLPDVIHHLETQHKRLGKL
                     IGELSEEQLDRSSASNPKRTVRSAILHGLHDEACHCGEIYLLGKMQVAARKGSSAGA"
     gene            complement(2387..2701)
                     /locus_tag="VHS97_07940"
                     /pseudo
     CDS             complement(2387..2701)
                     /locus_tag="VHS97_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
     gene            2888..3532
                     /locus_tag="VHS97_07945"
     CDS             2888..3532
                     /locus_tag="VHS97_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448172.1"
                     /translation="MDTSSSDRIADGSRSTPSRRDFLHQTAGIADTIATMTPSVQAAD
                     GPGSTSSLLPTIQLGPHKITRLIIGGNPVYGYSHFNKNFDRHMAAWHRPERVMELLKR
                     CEECGLNTFQNSYSKRTLADLERYRSAGGTMNGLCLGNPDWDQHPEFIDDAAEHKPIG
                     IAPHGSLNDRLYRQKKYNVLTDLLKRIRDHHGSNRGACPPIIEKADGCLSFGLI"
     gene            3855..4505
                     /locus_tag="VHS97_07950"
     CDS             3855..4505
                     /locus_tag="VHS97_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448173.1"
                     /translation="MSLIPTYIVLQRWADPRVVGHLDYTILFMLGGGLWIFVASRFLE
                     VLGISIRDDAIERDNLAALVAVCGILSGVGIVYALSNIGTGATIWTTLVPGFVATAAL
                     LLMVLLVELTGGAVADAITIDRDVASGLRLGGAVLGCAIILGHAATGDWTSWYHTWSD
                     LVTRGWPAVLVAVAAGVLQRMLGPTAHRPQPGILKFGVVPATLVVAAGFLVVIIWR"
     gene            4587..5765
                     /locus_tag="VHS97_07955"
     CDS             4587..5765
                     /locus_tag="VHS97_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX3448174.1"
                     /translation="MPEDLFQGVRHRLVLDAFKWDPQVGDVSTISRFPLLLGKTHWGQ
                     LSHLATLLAAELNAAERELLGRPDLHNRLGLPRGIRSVLRQAGKVGATHSAARVLRFD
                     FHWTRDGWRISEVNSDVPGGFSEASELPALMATYNPRTQLAGNPAAAWANAIAAAVPT
                     GHPIALVVAAGFLEDQQVVAYLAHLLGQRGITSHWATSADLRWKFGHAYLESRVSLGP
                     IGAIVRFFQAEWLNARSCAPLFVGGQTPVCNPGIAVLTESKRFPLVWDHLSTALPTWR
                     RLLPTTADPRDADWLRDDSWLVKSALCNTGDTVVIRSLLSNDQWRSATRAVRRHPGAW
                     VAQRRFAACPIQTPAGPIYPCIGVYTVNGCPTGIYGRFSYTPVIDFAAVDVAILIADD
                     "
     gene            5778..>6282
                     /locus_tag="VHS97_07960"
     CDS             5778..>6282
                     /locus_tag="VHS97_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448175.1"
                     /translation="MTKQSIFDAWAPPSAIWSRWAKPVLFSQMDSVARDENLVPVLPV
                     DVSWAVDSDRATALVVDLPGAQGVTMAMALAAMGYRPVPLYNACPAPAGDTALVVVRP
                     IMIALASAASSLQSLGLPDEATPAFLLDAMRNPSAMTPPPDIVDDRFTSLPSFLMDAK
                     GDTAVVAP"
BASE COUNT         1239 a         1816 c         1809 g         1418 t
ORIGIN      
        1 atgtggacca ccggcggtga tccagaagag gttggcatct cgcaccggta acttcatgaa
       61 cccggcgctg ctcccgcagt cgctctcgtt gctcttgggg cagacgccgc cttccgagcg
      121 ggagccagac gggtcataat cggttcaaga tgaccttcac aggtgcaagt tcggcgctca
      181 acagccgggt atccttcccg ccgctccgat gacaatagac gtgagtcgca aggaaaaacc
      241 atgctacgca agctgatcat caagccacac tcagcagccc cggacgcaac gaccggcgag
      301 atcgacatag ctgcggtcgc cacagtgctg gtgacctcag aggttcccga ccatcccatt
      361 gatcgtgcct ttgaccccca tcggggaccg ggaggcactc gctggatcgc cggggagccg
      421 ggtgagcaga gcgtgatcct tgccttcgac gccccgcaga cgatccgaca catcctcctg
      481 gaaatcgagg agcccgaagt cgctcgcaca caggaactcc agttgagtct ctcttgcgat
      541 ggaggacgga cataccggga actgctgcgg caggaataca acttcagccc ggccggaaca
      601 accttcgagc gggaagactg ggcggtgaca gccgagagcg tcacgcacct gcggcttgtc
      661 atcaagccag ataatggggg caagccctgc tgggcaaccc tcacctcgct ggccttgcgg
      721 tgacggagcc aaggtggcgg acgaggctcg atctcaggaa tgggacgacc aaattacctg
      781 cccggtgcgc tagcccgagc tttctatcaa tactttccat ttcttgttat ccttttgtcg
      841 gttggtctgc cgacagagtg gaaccgctgg gtcttcccga tccggtcgat gcttctccct
      901 cgtcttatcc acccaatggg cttcgacgga agcccctttc ttctcgaccc tgaaagcatc
      961 ccgagggtcg tcaaactctc gtttcggagt tcgatcgtgg gtaagaagct gtatgtcggc
     1021 aacctgactt acaacgtcaa cgagtccgac cttgaggcgt tgttcacgcc attcgggtcg
     1081 gtccagagtg cacaggtcat cgtcgatcgg gacaccaacc gctcgaaggg gttcggattc
     1141 gtcgagatgg ggtccgacgc cgaggcgcaa gcggccatcc agggtctcaa cggtcatgac
     1201 tacgacggcc ggaacctgac ggtcaacgaa gccaagcccc gtgaggaacg tggcggcggc
     1261 ggtcggggcg gctacggcgg cggtggtggt ggtggtggtg gttatggagg cggtggcggt
     1321 cggcgttatt gagcattcga tcttgctgca ggggtcaaga agccttttca acggggctcc
     1381 ttgatcgacc tgcccgccat catcgctcat cgggtgttct ttccggacct agagtcgcac
     1441 tgatccgctt cgcccccact gccaattcgt cgatccagag tgtctggtcg tgcggcacac
     1501 cgtcgtggaa ataaggcagc atcatgaact gattgaactg catcttgggg aaatcgatcg
     1561 agcggaacag cacgtcggac cgctcgatga taacgctccc gtcgacccag ccccgaagtt
     1621 ctccgacgtg attcagcttg tcccaagcca cgtcccggct gttgagcttg aacatcgcct
     1681 cgacgcagtg ccatcgttaa acaagacctt ctcgctatcg aagaactgcc cgttgtagcc
     1741 accccgcagc ggtccctggg tcaacccgtg gagcatgtcc cggttctgga tatcttgagc
     1801 cgctaaccgc agcctgccgt tgacggtctc gatgtagagc gtcaggtggg tggcggcgcc
     1861 agggaatcca tggcaacaca tttagggcaa tgaattgggg cttgtcccag gatcacgccc
     1921 cggcgctaga gccctttctc gcagccactt gcatcttacc gagcaggtag atctcgccgc
     1981 agtggcatgc ctcgtcgtgc aatccgtgga ggattgcgga acgcacagtg cgttttggat
     2041 tgctcgctga cgaacgatcc agttgctctt cactcaattc accgatgagc tttcccagcc
     2101 gcttgtgctg cgtttcgagg tgatgaatga catcaggaag ttgcggccaa cgatcggcag
     2161 gcaccaagtt cgggtggctc tccggactga acatctcgta ccagccatca gggatcaaga
     2221 tcgatgtcct cgacgagagt tactttcgcc ccgcttgcct cggggaatga aggcggctga
     2281 tctgtcggaa cgccagcgac ggcagcctcg atgaaattgg taactcggat gagcgctggc
     2341 aatcttcgat gttgtacatg aggattttat ttttccagtg gtcgtctcag ctcctctgcc
     2401 acctgacacg ccagtcgatg ctctggatcg ccgaggcatc atccgtccac ttggctccga
     2461 gtaggcggcc gatatctttg aggctattcg agtaagtgca gaagtagaaa tgtgtgtaga
     2521 taatcgagag aacgttgacg agggatgtga tcgcctgatc ggcgaattcc gtgcgggttg
     2581 tttgttttcg catccgctgg aggaaagtct tctcgtagct gccatagcag aatagcgtga
     2641 actcgcccag gctctgcaac tcgtcaagga gtaggtcgaa gatcctgctc tcttcatcgt
     2701 ggccatcggc ccaaaacgat cagcagatct gaacgtgcgt ctctcattat aggtcaatcg
     2761 aggtgcaatg gccgcaggta ttgccgtcgt tcccggcttc gaggtgtccg tgctggttct
     2821 ttctctggaa gaactctgtt aatctgaggc cgtccccatc ttctaccgag ccaggaaatg
     2881 gtctttcatg gatacatctt catcggatcg tatcgctgat ggcagccgtt ccactcccag
     2941 tcgccgggat ttcctgcacc aaactgcggg gattgctgac accattgcta cgatgactcc
     3001 ctcggttcaa gccgccgacg gacctggctc aacttcgtcg ctattgccaa ccattcaact
     3061 gggtccgcat aaaattactc ggctaatcat cgggggcaat ccggtctacg gttattcgca
     3121 cttcaacaag aacttcgacc gtcacatggc cgcctggcac aggccggagc gagtcatgga
     3181 attgctcaag cgatgcgaag agtgcggact caacaccttc cagaacagct actcgaagcg
     3241 aacgctcgcc gacctggaac ggtatcgctc tgcgggcggc acgatgaatg ggctctgcct
     3301 gggcaatccc gactgggacc agcaccccga gttcatcgac gacgctgccg aacacaaacc
     3361 catcggcatc gccccacacg ggagcttgaa cgataggctc tatcgtcaga agaaatacaa
     3421 cgtcctgacc gaccttttga aacgaatccg ggaccaccat ggctcgaatc gaggagcgtg
     3481 tccccccatc attgaaaaag cggacgggtg cctctcattc ggtctaatat aatcaattta
     3541 ggccccgata gggttccagg atctggcttc gcgtatggta tcaggccttc tgacgcctgc
     3601 gatcaggaac caaatcctgg aactgcagtc gtgcagagca taatcgaagc taaacgcctc
     3661 caacccgcgt cgtgcgacga gcggagaccc tggcgatgtc gtactttgac tttgatggtg
     3721 acgaatggct cttctttttt gccgccgctg caagtgcaat cgttctaatt ataatatatt
     3781 atccaccatt gctattaata tcacccatca agaggtcgag tgttcaacgt atcgctctcg
     3841 cgtgtctccc cttggtgtcg cttatcccga cttacatcgt attgcaacga tgggcggatc
     3901 cccgagtcgt cgggcatctt gattacacca ttctcttcat gctcggtggc ggcttgtgga
     3961 tattcgtcgc gtcccggttc ctcgaggtgc tgggaatcag catccgggat gatgcgatcg
     4021 agcgggataa tctcgcggcg ttggtcgccg tgtgcggaat attgtctggt gtcggaattg
     4081 tatacgccct ctccaacatc ggcacgggag cgacaatctg gacaacgctc gttcctggat
     4141 ttgtcgcaac tgctgctcta ttgttgatgg ttttgcttgt tgaattaact ggcggagccg
     4201 tggccgacgc gatcacgatc gatcgtgatg ttgcctccgg gctaagactg ggcggtgcag
     4261 tgcttggctg tgcgatcatt ttgggacacg ccgcgacggg cgattggacc tcgtggtatc
     4321 atacgtggag tgatcttgtc actcgtggtt ggccggcggt cctggtcgcg gtcgctgccg
     4381 gagtcttgca gcggatgctt ggacctacgg cccatcgtcc ccaaccgggc attcttaaat
     4441 tcggcgtcgt cccggcgaca cttgttgttg cggcgggctt cttggttgtc atcatttggc
     4501 gatagcggtg tggattccaa tcaattgcga tccgagaaca accgacttca atggcggaca
     4561 cagagcccct tcgagtaggc gatgaattgc ctgaggatct ctttcaaggt gtgcgccatc
     4621 ggttggtcct ggatgccttc aaatgggatc cccaggttgg tgatgtctca accatcagtc
     4681 gatttccatt actgctcggc aagacgcact ggggtcagct atcccatctc gcaactcttc
     4741 tcgccgcgga actgaacgca gccgaacgag agctcctcgg gcgacccgat ttgcacaatc
     4801 gacttggttt gccgcggggt attcggtcgg ttttgaggca ggcagggaag gtcggcgcaa
     4861 cgcactcggc ggctcgtgtg ttgcgattcg attttcattg gacgcgtgat ggttggcgca
     4921 tctccgaggt caacagcgat gtgcctggcg gtttcagtga agcctcagaa ttgcctgctt
     4981 tgatggcaac gtacaatcca aggacccaac tagcgggcaa tccagccgcg gcgtgggcca
     5041 acgcaatcgc ggctgccgtc ccgaccgggc accccattgc gctggtcgtg gccgccggat
     5101 ttctggaaga ccaacaagtc gtcgcatacc tggctcactt gttaggccag cggggaatca
     5161 catcgcattg ggctacgtca gcagatcttc gctggaagtt cggtcatgcg tacctcgagt
     5221 ctcgcgtgag tctcggtcca ataggcgcga tcgttcgctt cttccaggcg gaatggctta
     5281 atgcgcgctc gtgcgctcct ttattcgtcg gtggtcaaac gccggtgtgc aatccgggca
     5341 ttgcggtact cacggaaagc aaacggtttc ccctcgtgtg ggaccattta tccacggccc
     5401 tgccgacctg gcgccggctt ctcccgacca ctgcggatcc tcgcgatgcc gactggcttc
     5461 gagatgacag ttggctcgtc aagtctgcgc tttgcaatac cggcgacacg gtcgtgattc
     5521 gctcgctcct gagcaatgat caatggcgca gcgcgacgcg agccgttcga agacatccgg
     5581 gtgcatgggt ggctcaaagg agatttgcag cgtgcccgat tcaaacgccc gccgggccaa
     5641 tttatccatg catcggcgta tacacagtga atgggtgccc gacagggata tacggtcggt
     5701 tcagttacac tccggtgatt gactttgccg ctgtcgacgt tgcgatattg atcgcggatg
     5761 actaggacaa acgaaccatg accaagcaat ccatattcga cgcctgggca cccccctcag
     5821 cgatctggtc gcgatgggcg aagccggttt tgttttccca gatggattca gtcgcgcgag
     5881 acgagaactt ggttcccgta ctgcccgtcg atgtgagctg ggctgtcgac tcagatcgcg
     5941 caacggcgct ggttgttgat cttccaggcg cacaaggagt aactatggcg atggccctgg
     6001 ccgccatggg ctatcgtcct gtcccgctgt acaacgcgtg tcccgcaccg gcaggtgaca
     6061 ctgcgttggt agttgttcgt ccgatcatga tcgccctggc ttccgccgca tcttcgctgc
     6121 aaagccttgg actgcctgac gaagccacac cggcgtttct gttggacgcc atgcgcaacc
     6181 cctccgcgat gacaccgccg ccggacatag ttgatgatag gtttaccagc ttgccatcat
     6241 ttctcatgga tgccaaaggc gacaccgccg tggtcgcacc gt
//
LOCUS       DATAQL010000460         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_198584, whole genome shotgun sequence.
ACCESSION   DATAQL010000460 DATAQL010000000
VERSION     DATAQL010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198584"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>2979
                     /locus_tag="VHS97_07965"
     CDS             102..>2979
                     /locus_tag="VHS97_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3448176.1"
                     /translation="MIEHASGDASPSDPWHRLERGDHCSQDELVEILCADQANRWRAG
                     ERIPAEAYFARHPNLAGDSEGAFELVYGEYLVRELLGECPKFDEFLWRFPRFADRLHR
                     QLNPHQALADEEATKKPDSPPEMSEAAGEPSAILPELPGYQFLEEVGRGGMSVVFLAR
                     QVGLNRLVALKLIRARVYADAMIAARFRDEAEAAARFHHPNIIQVYEVGEHDGQGYLA
                     LEYAAGGNLEQRLAGTPQAPRDSARLVEDLARALHYAHQRGIVHRDLKPANVVLTEDG
                     VPKVTDFGLAKLLEREGGLTQTGDIMGTPSYMAPEQARGTPADVTPASDIYALGTILY
                     EMLTGRPPFKGSTPLSTLNQVADQEPLAPSKLQRHTPREIETICLKCLEKEPRKRYAT
                     ALDLADDLRRFLENRPIQARRISLPEWLWRWCRREPVNAALVVALFCAFIAGGTGVTA
                     QWRRAEQKARAESLARAGAERERGKALDNLYFSRIAQARLEWRLNNVAGVEQLLNKCD
                     QDRRGWEWHYLRNISHSEILGFDLPELSYIFGVVFSPDGSRFAFTGFDPYRQGNERSP
                     SLVEIWDTKNQLRLHSLDGPAHTMRLSFNHDGQRLAASGARETKIWDTASGQMVRAWS
                     PEGSVTFSPDGRILAAGGSKEAIFWDAENGKEVRRFSKSRGRVTFSPDGQMLAVSAPS
                     AVELRDVATGREIVSLAHGPGEDAARIERFFDGEGPELAFSPDGKYVVVATRPPRIWD
                     ASTGLPLHQLSGHAGSVPGVAFSPDGRQVATAGVDSTIRLWDVQTGTERAVLRGHSAL
                     VGCVAFHPDGWCLLSGGRHLGQVKLWDLTHPQENLTLTDCHSPAFAFDDDGRQLSLVT
                     NLGGLQVREIASGSTRVGAQVDMTMQWLTPAAPAEFSLKGRRFAAVSSNRLLVKVCDP
                     QSGAELITLRGLSAPATFVSLSRDGSLAAASGLYAKNEVRSREVK"
BASE COUNT          580 a          908 c          941 g          550 t
ORIGIN      
        1 ttcgtccggc gggcgcgccg acggcaatga gttgaacagg gaaggcgata tgtgcgtgac
       61 ctggcccttt tttggtggcg aactgatagg gcgaccgagc gatgatcgaa cacgccagcg
      121 gcgatgcgtc gccatccgac ccttggcatc gcctggagcg cggagaccat tgctcccagg
      181 atgaactggt cgagatcctg tgcgccgatc aggccaaccg gtggcgcgcc ggggaacgaa
      241 ttcccgccga agcatacttc gcgcggcacc cgaacctcgc cggcgatagc gagggggcct
      301 ttgagctcgt ctacggcgag tacctggtgc gggaattgct gggcgagtgc cccaagttcg
      361 atgagttcct ctggagattc ccccgctttg ccgatcggtt gcaccgccag ctaaatcccc
      421 atcaggcgct cgctgatgag gaagcaacca aaaagcccga ttctcctcca gaaatgtcgg
      481 aggccgctgg ggagccatcg gcgatcctgc ccgagctccc aggctatcag ttccttgaag
      541 aggtcggacg gggagggatg agcgttgttt ttctggcgcg acaggtcggc ctcaatcgcc
      601 tggtcgcact caagttgatc cgggccaggg tatatgccga cgccatgatc gccgcccgat
      661 tccgagacga agccgaggcg gcggcccgct ttcaccatcc caatatcatc caggtgtatg
      721 aggtcgggga acatgatggg cagggctatc ttgccctgga atatgccgcc ggcggcaact
      781 tggagcaaag gctcgcgggc acgccacaag cgccgcgaga ttcagcccgg ctcgtcgaag
      841 acctggcacg tgcactgcac tatgctcacc agcgcggtat cgtccatcgt gacctcaagc
      901 cggcgaatgt cgtcttaacc gaagacggcg tccccaaggt taccgacttc ggtctggcca
      961 agctcttgga gcgagaaggg ggcctgacgc agacgggcga catcatgggg acaccgagct
     1021 atatggcacc cgagcaggcc cgaggaactc ccgcggatgt gacaccggcc agcgacatct
     1081 acgccctggg aaccatcctc tacgaaatgc tcaccgggcg cccgccgttc aaaggatcaa
     1141 cgccactctc gacgctcaac caggtcgccg accaagagcc cctggcaccc agcaagctgc
     1201 aacgacatac gccccgcgag atcgagacga tctgtttgaa gtgcctggag aaagagccga
     1261 gaaagcgcta tgcgaccgcg ctcgacctgg ccgatgacct gcgccgattt ctcgagaatc
     1321 gtccgatcca agcccgtcgc atcagtttgc cggagtggtt atggcgctgg tgccgccgcg
     1381 agccggtgaa cgccgcgctg gtcgtcgctc tattctgtgc ctttatcgcc gggggaacgg
     1441 gtgtgaccgc ccaatggcgc cgtgccgagc agaaggcccg cgctgaatct ctggcccgag
     1501 ctggtgccga acgcgagcga ggcaaggcgc tagacaactt gtatttcagc cggatcgctc
     1561 aggcgcgcct tgaatggcgc ttgaataacg ttgcaggcgt cgagcaactc ttgaataagt
     1621 gcgatcaaga ccggcgcggc tgggaatggc attacttgcg caatatcagc cattcggaaa
     1681 tactcggctt cgacctcccc gagctcagtt acatatttgg cgttgtgttc agtcctgacg
     1741 gatcgagatt cgcattcacc ggttttgatc cctaccgtca gggcaacgaa cgtagcccgt
     1801 ctttggtcga aatttgggat acaaagaacc agctgaggct gcacagtctc gatgggccag
     1861 cgcatacgat gcgactctcg tttaaccacg atggccagag actcgccgcc agcggcgccc
     1921 gagagaccaa aatctgggat acggcgagcg gtcagatggt tcgtgcgtgg tcaccggaag
     1981 gaagcgtgac ttttagcccg gacggccgaa ttctcgccgc ggggggtagc aaggaagcca
     2041 ttttttggga tgcggagaac ggcaaggagg tgcgccggtt ctccaagagc aggggacgcg
     2101 tcacattcag ccccgacggc cagatgctgg cggtgagcgc accaagcgcg gtcgagctcc
     2161 gcgacgtggc caccgggcgc gaaatcgttt cgctcgcgca cggccctggt gaagacgcgg
     2221 cccgaattga gcgtttcttc gacggtgagg gccctgagct ggcttttagc cccgacggaa
     2281 aatacgtggt cgtcgcgaca cgcccgccac ggatctggga cgcgtcaacc ggcctgcctc
     2341 tccatcagct cagcggccac gccgggtctg tgccgggagt ggccttcagt cccgacggcc
     2401 gacaggtcgc gactgccggc gtcgattcga cgatccggct ctgggacgtc cagaccggca
     2461 ctgaacgtgc cgtactgcgt ggccactccg ccctggtcgg ttgcgtggct tttcatcccg
     2521 acgggtggtg cctgctctcc ggaggacgtc atttgggcca agtcaagctc tgggatctga
     2581 cccaccctca ggagaatctc actttaacgg actgccattc tccagcgttc gcttttgatg
     2641 acgacggcag acagttgagc ctggtgacga atctaggagg actgcaagtg cgtgagatcg
     2701 ccagtggatc gacaagggtc ggtgcccaag ttgacatgac gatgcaatgg ctcactcccg
     2761 cggccccggc cgaattctcg ctgaaaggtc ggcgcttcgc agccgtttca tcaaaccggt
     2821 tgttggtgaa agtctgcgac ccgcaatcgg gtgctgagct gatcacgctc cgaggcctgt
     2881 ccgcgccggc cacgtttgtc tccttgagcc gcgatggcag tcttgcagcc gcgtccggcc
     2941 tctatgcgaa aaacgaggtg cgctcacggg aagtgaagg
//
LOCUS       DATAQL010000461         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4931603, whole genome shotgun sequence.
ACCESSION   DATAQL010000461 DATAQL010000000
VERSION     DATAQL010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4931603"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHS97_07970"
     CDS             <1..223
                     /locus_tag="VHS97_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448177.1"
                     /translation="TTEGLSVQGRSQAHFFEAAGRLARPPAPPRARRTAPEIGIQDAR
                     AVDEVSLVRYPHFGGDVKIASKPPSGGFK"
     gene            complement(216..665)
                     /locus_tag="VHS97_07975"
     CDS             complement(216..665)
                     /locus_tag="VHS97_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX3448178.1"
                     /translation="MSVSSKCYYALRAVYALAEHPGSEPMKIADIAEREQIPIRFLEV
                     ILNQLKGGGFVQSRRGAEGGYRLARPADQLTVGEIMRYVDGPIAPVDCVSQSRPKVCE
                     FPGGCHFFCFWGRVRQAISDVVDKTTFADLIRESRERGNVHVGDWII"
     gene            complement(800..1420)
                     /locus_tag="VHS97_07980"
     CDS             complement(800..1420)
                     /locus_tag="VHS97_07980"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3448179.1"
                     /translation="MSTTQVHSPPKLGLESAGVPMTPEEFDAVEEWDECYRYELIHGV
                     LVVSPLPLEQEADPNELLGYLLRRYGDDHPQGSALNKTLPERYVRTQGSRRRADRVIW
                     AGLGRRPEPKIDVPTIVAEFVSEGKRSLRRDYEEKRQEYRAVGVLEYWVIDRFQRTMT
                     VFGNHPGVPPERIVAANEAYQTPLLPGFELPLVRLLAAADEWEETN"
     gene            complement(1490..1849)
                     /locus_tag="VHS97_07985"
     CDS             complement(1490..1849)
                     /locus_tag="VHS97_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448180.1"
                     /translation="MTHSTGFTEQDPSPPEDEENATPNRMFVFESGWRVGVKRGSTRE
                     FCHMTAPGQDFYHRLLDGEIFLTREAERLCFACASRRGLISYEPKRLRESIPCVPADL
                     EAIPLELDWRDAKRTNR"
     gene            complement(1846..>2263)
                     /locus_tag="VHS97_07990"
     CDS             complement(1846..>2263)
                     /locus_tag="VHS97_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX3448181.1"
                     /translation="ALITAADPDFVLQPIDLTAVEVATIDPTLTREDCMRSMHAVSGI
                     GRITAGFDAMRLLAAWLPIFWPLALIGYLPGVAWAGRRVYNRVAAARPRDAPCTDDSC
                     GIHSRTPRIVPRDRGHVQDHHKSIANLADTQEAHRP"
BASE COUNT          464 a          707 c          648 g          444 t
ORIGIN      
        1 aacaacggaa gggctgtctg tccaagggcg atcacaagct catttttttg aggcagccgg
       61 tcgactggct cgtcccccag ctcctccgcg agcgcggcgc actgcgcctg aaatcggcat
      121 tcaagacgcg cgtgccgttg acgaagtcag ccttgtccgc tatccccact tcggcggcga
      181 tgtcaagatc gcctcaaaac cgccaagtgg ggggttcaaa taatccaatc gcccacgtgc
      241 acgttaccac gctcgcggct ctcgcggatc aagtcggcga aagtggtctt gtcgaccacg
      301 tcggagatcg cctgccggac tcgcccccaa aagcaaaaga aatgacagcc gcccgggaac
      361 tcgcagacct tgggacggct ctggctgacg caatcgaccg gcgcgatcgg gccgtcgacg
      421 tagcgcatta tctcgccgac agttaactgg tctgcaggcc gggcaagccg gtagcctcct
      481 tcggcccccc gccggctctg aacgaaaccg ccacccttga gctgattgag gatcacttcc
      541 agaaatcgga tcgggatttg ttcgcgctcc gcgatgtcgg ctatcttcat cggctccgac
      601 cccggatgct cagccagcgc atacaccgcc cgcagcgcat aataacactt cgacgacaca
      661 ctcatgagcc cgatcccctt tatttgttat cgttccaggg aatcgttact atttcgaccg
      721 gagaaatcaa gtcggagcgc taaatccgaa ggcggggacg gaactcgacg gagagaatgc
      781 gaccgaaagc tgaactgcgt caattcgtct cttcccactc gtcggcggcg gcgagaagtc
      841 tgaccaaggg cagctcgaag ccgggaagta gcggagtttg atatgcttcg ttggcggcaa
      901 caatccgctc cggcggtacg ccggggtggt taccgaaaac cgtcatggtc cgctgaaacc
      961 tgtcgatcac ccagtactcg agcactccga ccgcgcgata ctcctgccgc ttttcttcgt
     1021 aatctcttct gagacttcgt ttcccttccg agacaaactc ggcaacgatc gttggcacgt
     1081 cgatcttcgg ttccggacgc cggcccaatc cggcccatat cacgcgatcg gccctccggc
     1141 ggctgccctg agtgcgtacg taccgctcag gcagcgtttt gtttagagcc gagccctggg
     1201 gatggtcatc accataacgg cgtaacaggt atcccagtag ctcgttggga tcagcctctt
     1261 gctccagagg aagtggactc acgacgagca ctccatgaat cagctcatag cggtagcatt
     1321 cgtcccattc ctcgacagcg tcaaattcct caggggtcat tggcacgccg gccgattcca
     1381 ggccgagttt cggcggcgaa tgaacctggg tagtgctcat gagtcgtgat ccgtaagaag
     1441 tcgacttagt cgcgggcagt acctattcat cctacccgaa tttcatggat caacggttcg
     1501 tccgcttggc atcgcgccaa tccagttcga gagggatggc ctcgaggtca gcgggcacgc
     1561 acgggatcga ctcgcggagc cgcttgggct cgtagctgat cagaccgcgg cgcgatgcgc
     1621 acgcgaagca aagccgctcc gcctcgcgcg tcagaaaaat ctcgccatcg agcaagcgat
     1681 ggtagaaatc ctggcccgga gccgtcatgt ggcagaattc acgcgtgctg cctcttttga
     1741 cgcctacccg ccatcccgat tcaaagacga acatgcgatt tggtgtggcg ttctcttcat
     1801 cctctggtgg cgacggatct tgctcggtga aacctgtgga gtgtgtcatg gccgatgcgc
     1861 ctcctgggtg tctgccaaat tcgcaatcga cttgtgatga tcttgaacgt gccctcggtc
     1921 gcgaggcacg atgcgcggcg tccgagagtg gatcccgcag gaatcgtcgg tgcagggagc
     1981 gtcacgcggc cgagcggccg caacccgatt gtacacacga cggcccgccc atgcgacccc
     2041 cggcagatac ccaatcagcg ccaagggcca gaatatcggc aaccaggccg ccagtaacct
     2101 catggcgtca aacccagccg tgattcggcc aatccctgag actgcgtgca tggatcgcat
     2161 gcaatcttca cgtgtcaacg tgggatcaat cgtggcgacc tccacggccg tcaagtcgat
     2221 gggctggagc acaaagtcgg gatcggcggc cgtgatcaga gcc
//
LOCUS       DATAQL010000462         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4626202, whole genome shotgun sequence.
ACCESSION   DATAQL010000462 DATAQL010000000
VERSION     DATAQL010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4626202"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1846
                     /locus_tag="VHS97_07995"
     CDS             344..1846
                     /locus_tag="VHS97_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX3448182.1"
                     /translation="MIRRSRFDRCTRALMPTLFALFACTHVYGANKQESWDAIYLGGS
                     KIGYVHTYVEKVHYKGKEYQRVRIHIEQRLKRDKDISVVNLEYGTIETLDGEVLRLDT
                     RILAGDAKDIRAHGDVIGGKMNLILESGERQELVIPWSREVRGPYAPEQSMARKPMNE
                     HERRDLKMFIAELNKVCDITLTSGGIEPVVLGDGSSRPLLRVELTTRVDGKPRPEFDS
                     TMWVDSSGQALKGEQDIFGGIVMYRTTKEAAMSAGGPIQFDLIKSTVIKIGRVLPDPE
                     QTRQVKYRISLKSGDPKEAIPTDSRQTVQLEGKANEAILEVKTAGPLDGQPGPAEVDS
                     QYLKPNVLITSADSRVLSLANQATKGAVDPWEKARRINHWVFEHIRDKNFGVAFAAAN
                     EVARNLSGDCTEHAVLAAAMCRAVGIPARAVVGLVYVERLKGFGYHMWYEIYTNQRWV
                     ALDPTFDQSSVDAVHIKIAESSLEGVAPFEVFTPILRVAGKLEIEPLERR"
     gene            2018..3469
                     /locus_tag="VHS97_08000"
     CDS             2018..3469
                     /locus_tag="VHS97_08000"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3448183.1"
                     /translation="MKPRRLALPSSIALALLACGQNVAFGQTVPRPAGQNQKPDNAQR
                     PAPQTPVGQTAARLDAVVLAAIRSNPMTAAYPISTNLQKGKVVLSGIVGTKQVHDAAV
                     RMVIDMGIPFRDDLVIDTGAAGAVALSAGAAVGGYGGAMPGTLSNSSPYIYPPPLFAR
                     LDDPFFGFVPPLVSFPPWWRRAGQGGPTVQPRKDPAALPQASPAVATDSSRPSASPKG
                     SWQPMEVDPVKGQVEISVDELGGVFLRGIVASEQAGREIEDAARSVPGVTAVTSQFQT
                     VPRRVQADEAPPPAPPVPMPGPSASEEREPQPSSAAAPAIVPSRPKPTAAAPLALDAQ
                     PLTRRIVGALERRSQVGELPVKVRSTDGVVTLSGRVPSAYEAMLVYRAAQQTPGVTDI
                     IDRLEFTVPDENHANPLLQKGRPEDIEPYLASQIRRHVGDLAHVDRIQVRGDSIDLRG
                     TLQNAGDLDRLLAILRSIPLLHGFRLETDFKAD"
BASE COUNT          788 a         1177 c         1124 g          709 t
ORIGIN      
        1 gtcgtcagcg gccaggcacc gtcgcgccat gccaagcaac ttgccctcca ggcggcgctt
       61 gagctcctct cgagtgcgat gtttagcgag aaaatcacaa ttggttgatg ccgtgcgtga
      121 taacacttca cgcacaaccc ctttacagca aatcgcgggc gctcccatca ctcgcggcgg
      181 tcgtcgagtc tctcgtcgct ccattcaagt cgctcgtcgc acttgaactt gagagcaggg
      241 ccggtttcgg ccatagtaca ctcaagagcg cgggtacaat tcatttcgtg tccgcggctc
      301 acacctccac actcagggct tccggatggg aaggggtccg cgcatgattc gccgatcgcg
      361 gttcgatagg tgcacacggg cgctaatgcc cacgctattt gcgctattcg catgcacaca
      421 tgtatatggc gcgaacaaac aagaatcatg ggacgcgatc tacctcggcg gctcaaaaat
      481 cggatatgtt cacacatacg tggaaaaggt ccactataag ggcaaagaat accagcgggt
      541 tcgcattcat attgagcagc ggctcaagcg cgacaaggac atttcggtcg tcaacctgga
      601 atacgggacg atcgagactt tggacggcga ggtgttgcgt ttggacactc ggattctggc
      661 cggcgatgcc aaggacatcc gtgcccacgg cgacgtgatc ggtggcaaga tgaatctgat
      721 cctggaaagc ggcgagagac aagagctcgt gattccctgg agccgcgaag ttcgcggtcc
      781 atatgcgccc gaacagagca tggcccggaa gcccatgaac gaacacgaac ggcgggattt
      841 gaagatgttt atcgccgagc tcaataaggt ttgcgatatc acgctcacct ccgggggaat
      901 cgagccggtc gtcttgggcg atggctcctc ccgtccgctc ctccgagtgg agctgacgac
      961 ccgagtcgac ggcaagccca ggcccgagtt cgacagcacg atgtgggtcg attcgtccgg
     1021 ccaggccttg aagggtgagc aggatatctt cggcggaatc gtcatgtacc gcacgaccaa
     1081 ggaagcggcg atgtcagcgg gtggaccgat ccagttcgac ctgatcaaga gcaccgtcat
     1141 caagatcggg cgtgtgctcc cggaccccga gcagaccagg caagtcaagt accgcatctc
     1201 attgaagagc ggcgatccta aagaggcgat tccgaccgac tcccgccaaa cggttcaact
     1261 cgaagggaaa gcaaacgaag ccatattgga agtgaaaacc gccgggcctc tcgacggtca
     1321 gccgggaccc gcggaggtcg attctcaata tctgaagccc aacgtgctca tcacaagcgc
     1381 ggatagccgc gtgctctctc tggccaacca agcgaccaag ggcgcggtcg atccttggga
     1441 aaaagctcgg cgaatcaacc attgggtttt cgagcacatc cgggacaaaa acttcggtgt
     1501 cgcctttgct gcggcgaacg aagtcgcccg caatctgagc ggcgattgca ccgagcatgc
     1561 cgtcctggcc gccgcaatgt gccgagcggt cggtattccg gctcgcgccg tggttggact
     1621 ggtttatgtc gagcggttga agggattcgg ctatcacatg tggtatgaga tctatactaa
     1681 ccagcgctgg gtcgctctcg atccgacctt cgatcagtcg tccgtcgacg cagtccacat
     1741 caagatcgcg gaatccagcc tggagggagt tgctcccttc gaggtcttta caccgattct
     1801 gcgcgtggca ggcaaactcg agatcgaacc gctcgagcgg cgctaaggta accggctcta
     1861 tagacgagtg acatctggct cgaaatcgcg acggtttcta acgaattagg tgaggcagac
     1921 ttgtcggctc gaagggcgaa tcgtagactg cgctaaagag ggcatcccga accaattgca
     1981 ccaagccagc cctccacgtc caggggggga cgacaccatg aagccgcgac gattggcctt
     2041 gccgtcatcc attgcgctgg ccttgctggc ctgcggtcag aatgtcgctt tcggtcaaac
     2101 tgtcccgcga cctgcgggcc agaatcaaaa accagacaac gcccagcgtc cggcaccaca
     2161 gacgccggtt ggccaaaccg ccgcgcggct ggatgccgtc gtactggcgg cgattcgctc
     2221 caatccgatg acggccgcct acccgatttc aaccaactta caaaaaggaa aagtggtcct
     2281 gtcgggaata gtcgggacca agcaagtcca cgacgcggct gtgcggatgg taatcgatat
     2341 gggcatcccg ttccgggatg atctggtgat cgacaccggg gccgccggcg cagtcgccct
     2401 aagcgccggg gccgctgtgg gaggatatgg cggcgccatg ccgggaaccc tttcaaattc
     2461 ttctccctac atctacccgc ccccgttgtt tgcccgtctg gacgatccct ttttcggatt
     2521 cgttccccca ctcgtgagct tccctccctg gtggcggcgc gctggtcaag gcggccccac
     2581 ggtacagccg cgcaaagacc cagccgccct gcctcaggca tcaccggccg tggccaccga
     2641 ttcctcgcgt ccgagcgcga gtcccaaggg atcgtggcaa ccaatggagg ttgacccggt
     2701 caaggggcaa gtcgagatca gcgtcgatga actggggggt gtattcttgc gcggaatcgt
     2761 ggcgagcgag caggccgggc gcgagatcga agatgccgcg cggagcgttc cgggcgtgac
     2821 agccgtcacg agccagtttc agaccgtccc ccgccgtgtc caagcggatg aagctccgcc
     2881 gcctgctccc cctgttccca tgccgggtcc gtcggcgtcc gaggaacgcg aaccgcagcc
     2941 atcatcggcg gctgctcccg cgattgtgcc atcgcgtccc aagcccacgg cagcggcgcc
     3001 tctggcactt gatgcccaac ccttgactcg ccgcatagtg ggcgcgcttg aacggcggtc
     3061 gcaggtgggc gagcttcctg tcaaggtgcg ctccaccgac ggcgtcgtca cactgtccgg
     3121 ccgggtcccg tcggcttacg aggccatgct cgtgtaccgg gcggctcagc aaacaccggg
     3181 agtgaccgac atcatcgatc gcctcgaatt cacggtaccc gacgaaaacc acgccaaccc
     3241 gctcttgcaa aaaggccgcc ccgaagacat cgagccctac ctggcatcgc agattcgccg
     3301 gcacgtcggt gatctggccc acgtggaccg catccaggtc cgcggcgatt cgatcgattt
     3361 gcgcggaaca cttcagaatg ccggcgacct cgatcggctc ctggcaattt tgcgttcgat
     3421 cccgttacta cacggctttc ggctcgaaac cgacttcaag gccgactaac aacaccgtcc
     3481 gacggagttg atctcgccgc cgccgccgac tgacccggcc gccacgcgca tcgagggatg
     3541 atgccccacg tctccgcgca atagcgctta acactcctcc ggcaatcggc gccgagccgc
     3601 gtgcccgccg ttcgtccgcg gtgtttcatt caatgctcgc aaaataggca aaatctaggg
     3661 ccggccttgt ctagaaatca ttcaaaatgc tctgggaacg gggggtagtt tgatcttgat
     3721 cgaatacgtg gaacgcacgt aggatgagcg ccgcccgcag gtaaggcaaa cgtggtcaag
     3781 cagttgttat ttgtccct
//
LOCUS       DATAQL010000463         6927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_505647, whole genome shotgun sequence.
ACCESSION   DATAQL010000463 DATAQL010000000
VERSION     DATAQL010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505647"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VHS97_08005"
     CDS             <1..990
                     /locus_tag="VHS97_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448184.1"
                     /translation="VLDYVGAWRQRMLDNGGIIPTNIGLDGKIGGACGGKWYGGVYGW
                     GFSVINPVTRGRVNRNYHHVAINGFGNAFLLTGDDRFLDAWRKQLDAVNSHAKVEGGR
                     SLYPHMHGDQGWYDFRPEPYRAGALELYYWSMKEPDRRRVPDDAWFTFLEGRNPAYPE
                     TALRGDFAVIRRKVEAMRRDTTTPDTRLADDPMAYNPATVGSLVHLMLGGLPPKHQGE
                     VLQARIRYFDPIRRRPGLPEDVAALVDSLKEDSTSLVLVNLNQAEARTLVVQGGAYGE
                     HLCERVETGERTTAVSHPSFRVDLAPGSGGRLTVAMKRYTVQPTLAPPWDHDD"
     gene            1193..2329
                     /gene="dinB"
                     /locus_tag="VHS97_08010"
     CDS             1193..2329
                     /gene="dinB"
                     /locus_tag="VHS97_08010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX3448185.1"
                     /translation="MPEIELWKEGIAKPDQGVAVEPARQRKIIHIDMDAFYASVEQRD
                     NPELRGQPVAVGGSRERGVVMAASYEARKFGIHSAMPSVTAKRKCPELIFVMPRFDVY
                     KSVSHQIREIFADHTTVFEPLSLDEAYLDVTENRKNIGSATEIAEIIRAKIRSETGLT
                     ASAGVSYNKFLAKMASSQSKPDGLFVITPKIGAAFVEKLPIGKFHGVGPATSAKMERL
                     GIRTGGDLRAQTLAFLQQQFGKAGPYYFWIARGIDERPVCTDRVRKSVGAENTFFTDL
                     FTVDEARAALNPLIEKVWRYCEGTPIRGRTVTLKAKYADFQQITRSRTSQAPFTTRAA
                     VEEIVYELLGPLFPVKKGIRLLGVTLSSLAEEPAGNEQQLRLSI"
     gene            2346..3650
                     /locus_tag="VHS97_08015"
     CDS             2346..3650
                     /locus_tag="VHS97_08015"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX3448186.1"
                     /translation="MCGARETKRDRRIFTRRSVLQGSLIGALVCYVRGEGASLRAAPP
                     SQSKKYDLLVKGGKVIDPSQDLSASRDIAVLGHKIAKVAMDIPEADAHHVIDVRGSIV
                     TPGLIDVHVHVYDGVAPLGIPPDPNCVAKGVTTVLDAGSAGAHTFPGFRKYVIDVAQT
                     RIHALLNISVVGQSTLSPDNMHGELLNLNYANPGLAVRTIEQHRDVILGIKVRLTRNI
                     AGENDLRALHLAREAAEAVKLPMMVHIGDTHTPLKDLLPVLRTGDVITHTFHGREGGI
                     LDEKGRVLPEVLTAVGRGVHLDVGHGAGSFSFAVAEQAINQGLLPGTISSDLHQFNIH
                     GPVFDLATTLSKFLHLGLSLEQVIARVTTHPAKTFGFAAGLGTLREGAEADVAVFSLQ
                     EGDFSFADSMGHRRVGHRKLTPVATVKSGRLYGAATFPVPGS"
     gene            complement(3674..4741)
                     /locus_tag="VHS97_08020"
     CDS             complement(3674..4741)
                     /locus_tag="VHS97_08020"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX3448187.1"
                     /translation="MIELPPAIGGPSAWYGPDIADSAEWIEPLSQAELAEIESVTKRL
                     AQTAIDWQNLRSQDFPLPNLSGRLARIGQEVLEGRGFVLLRALPVERWGQRLAAIAFL
                     GLGLHWGSLRSQNRQGHLLGHVKDLGLTSQDPNVRIYQTRERQNYHTDSCDVVALLCM
                     QPAKAGGLSSLVSSVTIFNEIRKGRPDLARVLFERIETDRRGEVPDGQGPFFSIPVFN
                     WYKGLLSTLYHRNYIESARRFSEVPPLTLEQVEALDLFDQLANDPALHFRMEFRAGDV
                     QLVHNHTLLHDRTAFLDWPEPERKRHLLRLWLAPTQARALPPVFAERYGSIVPGQRGG
                     ITVPGTRYTIPWEAEDAPHDV"
     gene            5286..6158
                     /locus_tag="VHS97_08025"
     CDS             5286..6158
                     /locus_tag="VHS97_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3448188.1"
                     /translation="MRLCPGIAVALATIMMVAGANEAPAGVISQWNFNTVESAPYNSP
                     ASDIGSGVATVLGMDNNYTFSSNNTITGTGTFTFTGPFSVGSTASADITPTAGDPNGF
                     ANAWRIRGPSNNLGDPNGNGWALQAPQYTQGAQFSSGTVGYSSISFHFDWFTTTQGVK
                     NLQEQYTLDGTHWININSPLTAVSNGWLAPQTIDFSGIAGANNDPNFGVRLVSVYDNS
                     LQFPNYGSADGSQGGYYNNNSGNWRFADVGFVGTAAAAVPEPSSFALSLIGIGLTGLI
                     VWQGKVRGSKLSAH"
     gene            complement(6162..6710)
                     /locus_tag="VHS97_08030"
     CDS             complement(6162..6710)
                     /locus_tag="VHS97_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448189.1"
                     /translation="MTHLVSLEFLQLLATVFLGVVGIVFTVLQASNAMRQNTLTLYEK
                     WDAQELSQYRSVVWDVWMNLKDGKDETVICWLGGDYFGMSIQEINQRIPEEQTHAVRS
                     LLNYFASVYEYQKLGLTDRKVTMQLFREPWLWWGDLFILLNKKVENHLRTKGQPESKW
                     AACSFITTLNRVAALDRVIRRP"
     gene            complement(6712..>6927)
                     /locus_tag="VHS97_08035"
     CDS             complement(6712..>6927)
                     /locus_tag="VHS97_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448190.1"
                     /translation="VFCETLRLSPMDSARMAQFGIDSAAGHSIWSDASHEGLEEFLAW
                     LGDPQAFSATHLRSVLDRVPADGNETG"
BASE COUNT         1426 a         2077 c         2060 g         1364 t
ORIGIN      
        1 gtcctcgact acgtcggcgc ctggcgccag aggatgctcg acaacggcgg gatcatcccg
       61 accaatatcg gcctcgacgg caagatcggc ggcgcctgcg gcggcaagtg gtacggcggg
      121 gtgtacggct ggggattttc ggtgatcaat ccggtcacgc gcgggcgcgt gaatcgcaac
      181 tatcaccacg tggcgattaa cggattcggc aacgcgtttc tgctcaccgg cgacgaccgt
      241 ttcctggacg cctggcgcaa gcagttggat gcggtcaatt cccatgcgaa ggtggaaggc
      301 ggccggtcgc tctacccgca catgcacggc gatcaaggat ggtacgactt ccggcccgag
      361 ccctaccggg ccggcgcgct cgagctctac tattggtcga tgaaagaacc cgaccggcgg
      421 cgcgtgccgg acgacgcctg gttcacgttc ctggaaggcc ggaacccggc ctatcccgag
      481 acggcgctcc gcggcgattt cgccgtcatc cgccgcaagg tcgaggccat gcggcgcgac
      541 acgaccacgc ccgatacgcg gctggcggat gacccaatgg cgtacaaccc ggcgaccgtc
      601 gggagcctgg tccacctcat gctaggcgga ctgccgccca agcaccaagg cgaggtgctc
      661 caagcacgga tccggtactt cgacccgatc cggcgaagac ctggcctgcc tgaagacgtg
      721 gcggcgctcg tcgattcgct caaggaggat tcgacgtcgc tggtcctggt caacctcaat
      781 caggccgagg cgcgaacctt ggtcgtgcaa ggaggcgcct acggcgagca cctctgcgag
      841 cgcgtcgaga caggggagcg aacgactgcc gtttcccatc cgtcgttccg tgtcgacctg
      901 gctcccggct cgggcggacg attgaccgtg gcgatgaagc ggtacacggt gcagccaacc
      961 ctcgctccac cctgggacca cgatgactga gagtctggca agcaggttcc gtttttgaaa
     1021 gactaacgac ccagttgctt ggaactggac gcatgaatag aatgggctga tcgatgcgga
     1081 ctgctcccgg tcgataaggg cgcggcgtga tgacattggc agttccgcaa tacgatcttt
     1141 tcgggaacga gatgaccgat gagggaggcc ttgcggcctg agtgggatgt cggtgccgga
     1201 gatcgaacta tggaaggaag ggatcgccaa gcctgaccaa ggcgtcgcgg tcgagcccgc
     1261 acggcagcgt aagattattc atatcgacat ggatgctttc tacgcctccg tcgagcagcg
     1321 ggataatcca gagcttcgcg gtcagccggt agccgtgggt ggatcacgcg agcgcggtgt
     1381 cgtgatggcc gcgagctacg aggcgcggaa attcggcatc cactccgcca tgccgtcggt
     1441 gacggccaaa cgaaaatgcc cggagctgat tttcgtgatg ccgcgattcg acgtctacaa
     1501 gtccgtgtcg catcaaatcc gcgagatctt cgcagaccac acgacggtct tcgagccgct
     1561 ttcgctcgat gaggcctatc tcgacgtgac cgaaaaccgg aagaacatcg gctcggcgac
     1621 cgaaattgcc gagatcattc gggcgaaaat ccgtagtgag accgggctca cggcgtccgc
     1681 tggcgtttcg tacaacaagt ttctggcgaa gatggcttcc agtcagagca agccggatgg
     1741 gctgttcgtc attacgccaa agatcggcgc tgctttcgtc gaaaagttgc cgatcgggaa
     1801 gtttcatggg gttggtcctg cgacaagcgc caagatggag cgactcggca tacgcacggg
     1861 cggcgatctc agggcccaaa cgctggcctt tcttcaacag cagtttggca aggcgggacc
     1921 gtattacttc tggattgcgc gcggtatcga cgagcggcca gtctgcaccg atcgcgttcg
     1981 caagtcggtc ggtgcggaaa acacattttt taccgatctg ttcactgtcg atgaggcccg
     2041 cgcggctctc aatccgctca tcgaaaaggt ttggcgctat tgtgagggaa cgcccatccg
     2101 cggtcgcact gtgaccctca aagcaaaata tgcggacttc cagcagatca cccgtagccg
     2161 aaccagccag gccccgttca caacccgagc agcagttgag gagatcgtgt acgagctgct
     2221 cgggccgctt tttcccgtca agaagggcat tcggctgctc ggggtgacgc tgtcatcact
     2281 cgctgaggag ccagccggca atgagcagca actccggctg tcgatctgag tcgggaggaa
     2341 gtgtcatgtg cggggcacga gaaacgaaac gcgatcgaag aatattcacg cgccgatcgg
     2401 tcttacaggg cagtctgatc ggggcgttgg tttgctacgt gcgcggtgaa ggagcctccc
     2461 tgcgcgcggc gccgcccagc caaagcaaga aatacgacct actggtcaag gggggaaagg
     2521 tcatcgatcc gtcgcaggat ctatcagcct cacgcgacat cgccgtcctg ggacataaga
     2581 tcgccaaggt ggcgatggac attcccgagg ccgacgccca tcatgtcatc gatgtcagag
     2641 gttcgatcgt gacgccggga ttgatcgacg ttcatgttca cgtctacgac ggcgtggctc
     2701 cgctggggat tccgcccgac cccaactgcg tcgctaaagg ggtcaccacg gtccttgacg
     2761 ctggatccgc cggcgcccac acgtttcccg ggttccgcaa gtacgttatc gatgtcgcgc
     2821 agacccgaat ccacgcgctc ttgaacatct ctgtggtcgg tcaatcgacc ctgtcccccg
     2881 acaacatgca cggcgaactg ctcaacttga actatgccaa cccagggctg gccgtgcgga
     2941 cgatcgagca gcatcgcgac gtgattctgg ggatcaaagt ccgtctgacg cgaaacatcg
     3001 cgggtgagaa tgaccttcgc gctctccatc tggcgcggga ggcagcggag gccgtaaaat
     3061 tgccgatgat ggtccacatc ggcgacacgc acacgccgct caaggacctc ctccccgtcc
     3121 tgcgcacagg cgacgtcatc acccacacct tccacggacg cgagggcgga attcttgacg
     3181 aaaagggacg ggtacttccc gaggtgctca ccgccgtcgg ccgcggcgtg catcttgacg
     3241 taggccacgg ggccggcagc ttctcgttcg ccgtggccga acaagccatt aatcagggct
     3301 tgctgccggg caccatctcg agcgatcttc atcagttcaa catccacggt cccgtgttcg
     3361 acctggccac caccctctcg aaattcctcc atctgggatt aagcctggaa caggtgatcg
     3421 cgcgggttac gacgcacccg gcgaaaacgt tcggcttcgc ggccgggctg gggactttgc
     3481 gcgagggagc cgaggccgat gttgccgtgt tttcgctcca ggagggcgac ttctccttcg
     3541 cggactcgat gggccatcgt cgcgtcggac accgaaagct gacgcccgtg gcgacggtca
     3601 aatccggtcg gctttatggg gcggctacct tccccgtgcc cggctcataa ggttgctttg
     3661 gccgggcgat tactcacacg tcgtgtggcg cgtcctccgc ctcccacggg atcgtgtatc
     3721 gcgtcccagg aactgtgatg ccgccacgtt gaccaggaac gattgagcca tatcgttccg
     3781 cgaataccgg cggaagcgcc cgggcttgcg tgggagccag ccaaagtcgc agcaggtgcc
     3841 gtttacgctc cggctctggc caatctagaa aggcggtgcg gtcgtgtagc aaggtgtggt
     3901 tatgcacgag ctgaacgtcg cccgcccgga actccattcg aaagtgcagc gcggggtcgt
     3961 tggccaactg gtcgaacagg tctagggcct cgacctgctc cagtgtcagt ggcggtactt
     4021 cggaaaaccg tcgtgccgat tcaatgtaat tgcggtgata aagggtcgaa agcagccctt
     4081 tataccagtt aaacaccggt atcgagaaaa acggtccctg gccgtcgggc acctcccccc
     4141 gacggtcggt ctcgatgcgc tcaaacagta cgcgggccag atccgggcgg cccttgcgta
     4201 tctcattgaa gatagtgaca gaactgacga ggcttgacag ccctccggcc ttggccggct
     4261 gcatgcacaa gagggcaacc acgtcgcacg aatcggtgtg ataattctgg cgttcgcgag
     4321 tctggtagat gcgaacattg gggtcctggc ttgtgagccc caagtccttc acgtgaccaa
     4381 gcagatgtcc ctgcctgttt tgcgaccgca gactccccca gtgcaggccc agtcccagaa
     4441 aggcaatcgc cgccaagcgc tggccccatc gctcaacggg cagcgcgcgc aagagcacaa
     4501 agccccggcc ttccagaacc tcttgaccga tgcgggccaa gcgcccgctc aaattcggca
     4561 atgggaagtc ctggctacgc aagttctgcc agtcgatcgc agtttgcgcc agccgcttcg
     4621 tgacagattc gatttctgcc aattcggcct gcgacaaggg ctcgatccac tcggcactgt
     4681 cggcgatatc aggaccgtac cacgccgaag ggccgccaat ggcgggagga agttcaatca
     4741 tgggtgatcg tctctaaacc taccgaaaac ttggagacgc ttctgcggtt taacttcctg
     4801 gaacgcgggc gtggcgatgg gctgcccagg cttggtctat actcgccatc gcggcgcggg
     4861 tcaagtttgg agacggcggg aacagatgcg tccactcgcg cggaacggtc gatgatgaac
     4921 tgtctccact tgttttccac tcctaaactt cgtttgacct gcgttgagcc atgttgttag
     4981 atcattctct ccgacgaatt gatctgatcc cagcagcggc ggacgagcgc cgttttcgat
     5041 tcctggtggt cagcgaaagt gaactgttcg caacgacctc gcgaactgag ccgatgccta
     5101 cgtggaattg actcccggtg gttatgatgg ctttccgcac agtatttggc ggacatcgcc
     5161 aatgaacgaa gctcggggaa aacaatagat ctccgacagg ttctatcact cgcgtttagc
     5221 cgaaccaatg agatctgatt cgataaagat ccgcttttca tcagtcactg ccaaggagtt
     5281 agagcatgag attgtgtccc ggtatcgcgg tcgcactcgc aaccatcatg atggtcgccg
     5341 gtgccaacga agcgcccgcg ggcgtcatta gccagtggaa tttcaacacc gtggaatcgg
     5401 ccccgtacaa tagcccggct tcggacatcg gctcgggcgt ggcgacggtg ctcgggatgg
     5461 ataacaacta cacgttcagc agcaacaaca ccatcacagg tacgggtacg ttcaccttta
     5521 ctggcccgtt ctctgtcgga agcacggctt ccgcggacat taccccgact gccggcgacc
     5581 cgaacggctt cgcaaacgcc tggcggattc gtggaccgag caataacctg ggcgacccca
     5641 atggcaacgg ttgggctctt caagctccgc agtacacgca gggggcgcaa ttctcttccg
     5701 gcacagtcgg ctactccagt atcagtttcc atttcgactg gttcaccacg acgcagggtg
     5761 tgaagaacct gcaggagcag tacactttgg acggtacgca ctggatcaac atcaacagtc
     5821 cgcttaccgc ggtgtcgaat ggttggctgg cgccacaaac gatcgacttc agcggaattg
     5881 ccggcgccaa taatgatcct aatttcggag tgcgcctggt ctccgtctac gacaactctc
     5941 ttcagtttcc caactacgga agtgccgatg ggagccaagg gggctattac aataacaact
     6001 cagggaactg gcgattcgcc gacgtcgggt tcgtcggaac cgccgccgcc gccgtgcctg
     6061 aaccgtcgag cttcgccctc tcgctgatcg gcatcggcct cacagggctg atcgtctggc
     6121 aggggaaagt ccgcgggtca aagctaagcg cccactgacg atcacggtcg gcggataact
     6181 cggtccaggg cggcaacgcg attcaaagtc gtgataaagg aacacgccgc ccacttgctc
     6241 tctggttgac ctttggttct caaatggttt tcgacctttt tgttcaagag tatgaaaagg
     6301 tctccccacc atagccatgg ttcccggaaa agctgcatag tcacttttct gtcagtcagt
     6361 cccagctttt gatattcata tacgcttgcg aaatagttaa gaagagatcg aacggcgtga
     6421 gtttgctcct cggggattcg ttggttgatt tcttggatgc tcatgccgaa ataatcacca
     6481 ccgagccagc agatgacagt ctcgtccttg ccatccttga gattcatcca gacatcccag
     6541 acgacgcttc ggtactgtga caattcctgg gcatcccatt tctcgtacag cgtcagggta
     6601 ttctgtcgca tcgcattgct ggcctgaaga acggtgaaga cgattccgac gacacccagg
     6661 aaaaccgtcg ccagaagttg caagaattcg agacttacaa gatgcgtcat gtcatcccgt
     6721 ctcgttaccg tcagccggca cacgatcgag cactgagcgg aggtgtgtcg ccgagaacgc
     6781 ttgaggatcc cctagccacg ccaagaactc ttccagcccc tcgtgcgatg cgtccgacca
     6841 aatcgagtgt cccgccgccg agtcgatccc aaactgggcc atccgggcac tatccatcgg
     6901 agatagccgc agggtttcgc agaaaac
//
LOCUS       DATAQL010000464         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3559452, whole genome shotgun sequence.
ACCESSION   DATAQL010000464 DATAQL010000000
VERSION     DATAQL010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3559452"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VHS97_08040"
     CDS             <1..313
                     /locus_tag="VHS97_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448191.1"
                     /translation="STTRYPISVLSATSGIASLQFDNSVRLVEKGYASMIRRPFIRAT
                     LGACLLIGSAFVAPGCSNDPAQPGRESISLPRKGGGVEAPAPAVKGKTSGGKLRDKGV
                     E"
     gene            435..1604
                     /locus_tag="VHS97_08045"
     CDS             435..1604
                     /locus_tag="VHS97_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3448192.1"
                     /translation="MHHHVSPSGRERRGFTLIELLVVIAIIAVLIALLLPAVQAAREA
                     ARRAQCTNNLKQLGLALANYESGNGSFPMAFFENFCELAAQGCGTGPCSSLGFGNGFG
                     PMVALLPYYEQAALFNAFNSSLEAFGDSNNTVDGAGVGTLWCPSDGSIQGYRATYQPG
                     EIYNNLIHYMCYSNYRGNWGYWDGSPTGTLGYTGAATVDVPHRIAAINQFNGVFVTNG
                     YGAAGGAVIPARAGIVRATVKIAGVIDGTSNTAAFSEIAHGLLSRTDYSPHGSFEDWN
                     WWTSGNLGDTTYAHFYPINPQKKMANIATDDQGGAFVNGASSFHPGGVNVAFVDGSVR
                     FIKESINTWAFAGSGYPLGVTRDQSVWQLAPGTKVGVWQALGSVNGGEVISSDAY"
     gene            complement(2346..2555)
                     /locus_tag="VHS97_08050"
     CDS             complement(2346..2555)
                     /locus_tag="VHS97_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448193.1"
                     /translation="MCTSTPSDADWWSIRRHGPGGVIPAMPIDDAVWSGWLTTNCWHR
                     GTARSVARTPATAYRRYVTAELSEL"
     gene            complement(2802..3110)
                     /locus_tag="VHS97_08055"
     CDS             complement(2802..3110)
                     /locus_tag="VHS97_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448194.1"
                     /translation="MANSLTNGQGTVLNDPPIPPIAAVPAIVSFDVRWSGVVSRANVT
                     NLASNFTGEYIETVAAIKWSAIQAGPPAFAFVSEDPNPARTFYAVIGRERNGVFFRRS
                     "
BASE COUNT          631 a         1033 c          998 g          688 t
ORIGIN      
        1 aagcacaact cgctacccga tctcggtcct ttcagcgacg agcgggatag cctcgttgca
       61 attcgacaac tctgttcggc ttgttgagaa aggctatgca tcaatgattc gtcggccatt
      121 catcagggct acccttgggg cctgcctgct cattggcagt gcatttgtcg cgccgggctg
      181 ctcgaacgat ccggcccagc ctggccgcga gtcgatctcg ttaccgcgta aaggcggtgg
      241 cgttgaagcc ccggcccccg cggtaaaggg caagacatct ggcggtaagc tccgggacaa
      301 gggcgtggag tagctcgcac cactttggtg cccgatgact gttcaagcct tcccgctcgc
      361 cgtaccgagt tcgtgaaacc gttctgattc tcttcatcct gatttgcagc ggtctcaatt
      421 tcaaggagaa gttcatgcac catcacgttt ccccctcggg ccgcgaacgt cgcggcttca
      481 ctctcatcga gttgctcgtc gtcatcgcga tcatcgcggt cctgatcgcg ttgcttttgc
      541 cggctgtaca ggcggcgcgc gaggcggcac ggcgggccca gtgtaccaac aacctcaagc
      601 agcttggcct ggcactggcc aactatgaga gcggtaacgg ctcgttcccc atggcgtttt
      661 tcgagaactt ctgtgaacta gccgcacaag gctgcggaac tgggccctgc tcgtcgttgg
      721 gcttcggcaa cggctttggg ccgatggtcg cactgctgcc ctactacgag caagccgcgt
      781 tgttcaacgc tttcaacagc tcgctggagg cgtttggcga ctccaacaac acggtcgatg
      841 gcgcgggcgt cggcaccctc tggtgcccca gcgacggctc catccagggc tatcgcgcca
      901 cctaccagcc cggtgagatc tataacaacc tgatccacta tatgtgctac tccaactatc
      961 gtggaaactg gggctactgg gacggatcgc ccacagggac actcggttac acaggcgccg
     1021 cgaccgttga cgtgccccac cgcatcgcgg cgattaatca gttcaacggc gtgtttgtga
     1081 ccaacggtta cggcgcggcc ggcggggccg tcataccagc ccgcgcgggc attgtccgag
     1141 ccacggtcaa gatcgccgga gtcatcgacg gcaccagcaa taccgcggcc tttagcgaga
     1201 tcgctcacgg gctgctgtcg cgcactgatt attcgcctca cggcagcttc gaggactgga
     1261 actggtggac ctccggcaat ctgggcgaca cgacttatgc tcacttctat cccatcaatc
     1321 cccagaagaa gatggccaac atagccacgg atgaccaggg cggtgctttc gtcaacgggg
     1381 cctcgagctt ccacccaggc ggggtcaacg ttgccttcgt cgatggctcg gttcgcttca
     1441 tcaaggaatc gattaacacc tgggccttcg ccgggtcggg atacccgctg ggcgtaaccc
     1501 gcgaccagtc ggtctggcag ctggctcccg ggacgaaggt tggcgtctgg caggccctgg
     1561 gctcggtcaa cggcggtgaa gtcatcagct ccgacgcgta ttgatctgga acgggccgaa
     1621 tgaatcggcc tcaagtcgag agaatccaat ccaggagacc gatcgggagg gtaggcgagc
     1681 ctgccttccg gtcggtttat tcgcagtgcg ccacccagaa tcggctgcac tcagaccact
     1741 ggacttcggt ggcgatgagg tgttcgtcta tgtcctcttc ctgatgaggc tccccgttgc
     1801 cgctgctctc gtctgcgttc ttggtctgtt gtgcgtgacc gttgccggaa tcgtcgccgc
     1861 ggtggagtcc gtgaccccga ctgggggcat gtcgcggtca gtccaagcgt caggccgagt
     1921 ttcaagttac tttgaatgct cccaggtcat ccaaccgttc ctccaagtgc cttagctgct
     1981 gccgaactct cgcgtcagtt tccagccaag cccgaaacgc cgggtcaggt tggggacgct
     2041 ctccgcgcgc gataccgcga gtatcggcgc cagctcggca ttggtcgcca ccgtcggact
     2101 tctcgcgaga taagccaatg ccgcgcgtgc tgggtgacgg ctgccgcgga ggctcagctc
     2161 cgcgcgctcg atttcaaaaa agcacagacg accttactta ctcgtgaaat gggcaggcct
     2221 tgtaacaatc gcgattcccg ccgctgctgt tggcgagttt tgccacgcac catcgcgcct
     2281 agtcgatcga tgaaagcacg tcttccgagg atccagccgt gatacgcctc ggttcaaggt
     2341 gactcttaca gttcggacaa ctccgcggtc acataccgcc ggtaggccgt cgccggggtc
     2401 cgggccaccg accgcgccgt cccacgatgc cagcagttcg tcgtaagcca cccactccag
     2461 acggcgtcgt cgatgggcat agccgggata actccaccag gcccatgccg ccggatgctc
     2521 caccagtcgg catcggacgg ggttgaggtg cacatatcgg gtcaccgtcc agagctaagt
     2581 ggacccgccg aagtcgtcgc gcagcgtagg tcgggctttc tagcccgacg ttttactcga
     2641 gtgcacaaca tgtcaggcta gaaaacctga cccacacgag taatcctgcg gtctcaccta
     2701 ggtctcgtcc tccaccagtt agactagaac cttggcctgc ccccatgggc tgtctctgga
     2761 atacgatacg gaccggctaa gaatcttctg ctgctgtaga actagcttcg gcggaagaag
     2821 accccgttgc gttcgcgtcc gatcaccgca tagaaagtgc gggcaggatt cggatcctcg
     2881 gacacgaaag cgaaagctgg gggcccggcc tgaatcgccg accacttgat ggcggcgacc
     2941 gtctcgatgt actcgccggt gaagttggac gcgagattgg tgacattcgc tcggctgacg
     3001 acgccactcc accgaacgtc gaaggagacg attgccggta cggcagcgat cggcgggatt
     3061 ggcgggtcgt tcaacacagt cccctgtccg ttcgtcaggg agttcgccaa gtcatgagaa
     3121 tccatgattc ggagatgatc gagccgaaac gaggccgttg cgttgtccag gtcgatctgc
     3181 acgctgtccg gcgggactgc aattgtccaa aacaagccgt tcgaagggat ccccggattg
     3241 taatcgtgga tctgctgagc cacattggtc gatccaactg gaccggtgta gatgtccagt
     3301 cagaggaagc cgaaagtgtt ttgatggagg cgtccgtcct ggccgatgta
//
LOCUS       DATAQL010000465         7418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3864441, whole genome shotgun sequence.
ACCESSION   DATAQL010000465 DATAQL010000000
VERSION     DATAQL010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3864441"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3677
                     /locus_tag="VHS97_08060"
     CDS             <1..3677
                     /locus_tag="VHS97_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448195.1"
                     /translation="IQLRKNNPSHPAGASSGFQGAERLDLLKNVHVVMRDVGNSDFMG
                     GKPSTRGAKAKKPAAKTTSDTKKADQAAAEPEQPTPLDLRCDSTMQIFLPKPRLPVLI
                     GPPAPPAPTLVQFERNVVVLRGPIDSWPDQLTCDTLKLSLIPAEKTPAENAQAAAGAS
                     DGSAREAGGNTKEAAEPDTGVQTVSRTGEPAVPDGDTQASTDGSKPKSEPKPDRESNN
                     GLLGGLTLQRAHATGHAVWLLLPAKGVKLRCNELIHFRQAPYTQKPDLTYFRGDRTRL
                     LEVEKVSVVEEEGPDQGKISSVTNIWTVDATMLDNGNGMDAADVVAHGPGRLETRPDR
                     GQPVERIAIWQDKLILQNELDGHGQVVRKFVDLSGDRPCFIDKLQDTMIDAGFLIKVT
                     LKPKATALPAVAKNDPSAAIFAGNAGTNTHNGGTGAMAAGADQTTSPAANTGAGVPRD
                     GGSADLAGGNLQIERLRAYRDVHLIAPARTMTARERLDAEFVQAESPQVASNGSTKSA
                     ASAPRKDEGRRMKDEASPDVAPAQGEPQVAAQDQPQVVADGQKEAEGQKEQEEREPPM
                     TGSCDRMWALVALKPKTESDPASSNEGKATKKSASARPGSGETNAEIRKVWMWGNVAL
                     HKDPAKGETKGEEASGEAIYLHNRGTNNALTYIYQSDPTEKTYLSGPLPPARVENAEI
                     NITAAGRIGMDQGADQAWVEGPGTLTQLTDRGFLTDKSPDPDEPEPAAPANDGKVTRT
                     GSAGPPVTLRTTAFVNADDIQENAPAPEEKVAATKPKTRAGKPLNNKVPMTIGFSERM
                     NFAGRSVDPEGRPAARIDFYGIVIAEMEDALLYAKEKMIAFTDKEVPLAQLGEMSKAK
                     KKRGAADGAQEAEEQDGEAKSSAELALIFCYRNAIAISRKVDPDFPRPIQQQRLEADK
                     ILAYDRRTGDFHVPGKGKVYLYDLSGKSSQTSGSNSGEESKKDSLPAVTETQRTVSST
                     SSRTTKPGTGKAATAPAPGTRGTTKNARSQRADQPAAAEGPSLVLTQVHFNKEMRGRY
                     GSGKETDTVETRWSEFFGDIETCRAKVPNTETQLNPDHLPVDGMFLTGQTLRVISEPP
                     PTGSPKTTPARTYMKAWENAYAWSNDRVLQGDVITYDSYKDLIYAYGENGRKVIFAEQ
                     YALGQPTSPGASSAVQLNPKTGTIHLINSDTLQMLDKKTGSRPGMAGQIDPNPVKPKK
                     TKRPFKQPTQSQERKGFTGT"
     gene            complement(3854..4840)
                     /locus_tag="VHS97_08065"
     CDS             complement(3854..4840)
                     /locus_tag="VHS97_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX3448196.1"
                     /translation="MIDPMDWPSPTLADVLKARRLIAPYLPVTPSLEPSGLALALGCR
                     AVIKCENLNPTGAFKVRGGVNLVAALDPDVRSRGVVAASTGNHGQSVAYAARLFGCKA
                     TIFMPEAANPLKVAATVALGAEVIQTGRDFDSARLAAEEFAARYLSRYVHSANEPLLV
                     AGVGTAALELLEAAPDVDVLFVPVGGGSGILGAGIVARAVNPAIRVVGVQAEGAPAVY
                     HSWREGRRVVTDSVNTFAEGLATREPFAMPLALFPRLVDEIVLVSDDEIAAAIRLLVT
                     TARQVVEGAGAAALAAAMKRRDQLAGKNVGLIVSGGNITIDQLSRIINHERP"
     gene            4995..5558
                     /locus_tag="VHS97_08070"
     CDS             4995..5558
                     /locus_tag="VHS97_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448197.1"
                     /translation="MALARIKPAPAKVVQSKSDNGSGVAVAGVPEVDDEEEEDEDEED
                     EDEDEDEDEEDEDEDEFVESRVIDTPGVCDSSLGGSLALVTLSEKFRTPPAASPWPKK
                     LRPSACSGLPFWSVVLKRKSNTPALLKPCEPGMIVSGPFVDVAGGVTTPPAGSMSATT
                     CGVVTLVAPAVGGGPVGAAILLVLVKV"
     gene            6454..7035
                     /locus_tag="VHS97_08075"
     CDS             6454..7035
                     /locus_tag="VHS97_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3448198.1"
                     /translation="MVQTLSDVQTPDDTLVRRARAGQSSAREELFHRHRGESYRYAYR
                     LLGNEQDALDVVQEAMLKAFSALADFDGRSGFRTWLLRIVTNTAYDWGRRRKRRPSAR
                     GGDEATSYEPTSDEDPAHRLNQQELRRELDQALNRLSSTLRETFILYAELGLSYKEIA
                     ETQDVPIGTVMSRIHAAREKLQAGLDWDKLSGL"
     gene            7060..>7418
                     /locus_tag="VHS97_08080"
     CDS             7060..>7418
                     /locus_tag="VHS97_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448199.1"
                     /translation="MQCDKVIRELAVPTDDRDPASLAEHLADCPSCAVWADRAARFDR
                     LWEATRPAEPNGEMWDTLWTQVSRAVDESTPVEQNAFTLHPSSLNGSAAKTEKSPAPK
                     GPSSGFRAGRLMAIGSLG"
BASE COUNT         1647 a         2244 c         2349 g         1178 t
ORIGIN      
        1 taattcaact ccgcaagaac aacccgagcc accccgccgg tgcatcgtcg ggctttcagg
       61 gagctgagag gctggacctg ctaaaaaacg tgcatgtggt gatgcgagac gttggcaatt
      121 cggatttcat ggggggaaag ccttcgactc gcggagcgaa ggccaagaag ccggcggcca
      181 agacaacctc cgatacaaag aaggccgatc aagcagcggc tgagccagag caaccgacac
      241 cgctggacct gcggtgtgac tcgacgatgc agatctttct gcccaagccg cggctgcccg
      301 tattgatcgg tccgccggcg ccgccggccc cgacgctggt tcaattcgag cgtaacgtcg
      361 tcgtcctgcg cggcccgatc gactcatggc ccgaccagct tacctgcgat accctgaagc
      421 tgagcctgat tccggcggaa aaaacgccag cggaaaatgc ccaggccgcg gcgggtgcga
      481 gcgacggctc ggcgcgcgag gccggcggca atacgaaaga ggccgccgag cccgacaccg
      541 gggttcagac agtatcccga actggcgaac ccgcagtgcc tgacggcgac acgcaggcga
      601 gcaccgacgg ctcaaagccg aaatccgagc cgaagcccga ccgtgaatcg aacaacggtc
      661 tgttaggcgg gctgaccctc cagcgcgccc atgcgaccgg acacgccgtc tggctcttat
      721 tgcccgccaa gggcgtcaag ctccgttgca atgagctgat ccacttccgg caggctcctt
      781 acactcagaa gcccgacctt acttacttcc ggggtgaccg cacccggctg ctcgaggtcg
      841 aaaaagtctc cgtcgtcgag gaggagggac ccgatcaagg caagatctcg tcggttacca
      901 acatctggac tgtcgacgcg acaatgcttg ataacggcaa cggaatggac gctgccgatg
      961 tcgtcgctca cggccctggc cggctcgaaa cccggcccga tcgcggtcag ccggttgagc
     1021 gcatcgccat ctggcaagac aagctgattc tccagaatga gctggatggc catgggcagg
     1081 tcgtgcggaa atttgtcgac ctttcgggcg accgcccgtg cttcattgac aagcttcaag
     1141 acacgatgat cgacgcggga ttcctgatca aggttacgct caagcccaag gcgaccgcgc
     1201 tcccggctgt ggccaagaac gatccctcgg ccgcgatatt cgcgggcaat gccggcacga
     1261 acacgcacaa cggcggaacg ggcgccatgg ccgccggtgc ggaccagacg acttcaccgg
     1321 ccgcaaacac tggcgccggt gtcccccgtg acggaggctc ggccgatctg gccgggggta
     1381 atctccagat cgaacgcttg cgtgcttacc gcgatgttca cttgatcgcg ccagccagga
     1441 cgatgaccgc gcgcgagcgg cttgacgccg agttcgttca ggccgagtca ccgcaggtcg
     1501 cctcgaacgg ctccaccaag tctgccgcaa gtgcgcctcg gaaggatgaa ggccgaagga
     1561 tgaaggatga agcaagcccc gacgtcgccc cagcccaagg cgagccgcag gtcgcggcgc
     1621 aggatcagcc gcaggttgtg gcggacgggc aaaaggaagc cgagggccaa aaagaacagg
     1681 aggaacgcga gccgccgatg actggctcgt gtgaccggat gtgggccttg gtggctctga
     1741 aaccaaaaac ggagtcggat ccagcgtcgt ccaacgaggg taaggcgacc aagaagagcg
     1801 cttccgctcg gccgggatcc ggcgagacca atgccgagat ccgcaaggta tggatgtggg
     1861 gcaacgttgc cttgcacaag gatcccgcca aaggcgagac gaagggcgaa gaggcatcgg
     1921 gcgaggccat ctacctgcac aaccgcggta cgaataacgc gctcacctac atctaccaga
     1981 gcgacccgac cgaaaagacg tacttatcgg gcccgctgcc cccggcgcgg gttgagaatg
     2041 ccgagatcaa catcacggcg gcgggcagga tcggtatgga ccaaggggcc gaccaagcct
     2101 gggtcgaggg gcctggcacg cttacgcaac tgaccgatcg cggcttcttg acggacaagt
     2161 ctcccgatcc cgatgagcct gagccggccg caccggcgaa tgacggtaaa gtgacccgga
     2221 caggctcggc cggcccgcca gtgacacttc gaacaaccgc gtttgtcaac gccgacgata
     2281 tccaggagaa cgccccagct cccgaagaga aagtggccgc gaccaagccc aagacccgcg
     2341 ccggcaagcc gctcaataac aaggtgccga tgacgatcgg cttcagcgag cgtatgaatt
     2401 tcgccggccg atcggtcgac cctgagggtc ggcccgcggc caggatcgat ttctacggca
     2461 ttgtcatagc cgagatggaa gatgccttgc tgtacgccaa ggagaagatg attgccttta
     2521 ccgacaagga ggttccgctt gcgcaacttg gcgagatgtc caaggcgaaa aagaagcgtg
     2581 gtgcggccga tggggcgcag gaagcggagg agcaagatgg ggaggctaaa tccagcgccg
     2641 agcttgcgct catcttttgc taccgcaacg cgatagccat cagccgcaag gtcgatcccg
     2701 atttccccag accgatccag cagcaaaggc ttgaggcgga caaaatcctg gcctacgacc
     2761 gccgtaccgg cgactttcac gtcccgggca agggcaaggt ttacctctac gacctgagcg
     2821 gcaaatcgtc gcaaacttct ggttcaaatt cgggcgagga gagcaagaag gacagccttc
     2881 ctgcggtcac cgagacgcag cggactgtca gctcgacatc gagccggacg accaagccgg
     2941 gcaccggcaa agccgccacc gcgcccgctc ccggcactcg cggcacgacc aagaacgcgc
     3001 ggtcacagcg ggccgaccaa ccagcagccg ccgagggccc gtcattggtc ttgactcagg
     3061 tccatttcaa caaggagatg cgaggccgat acggaagcgg caaggaaacc gacacggttg
     3121 agacccgctg gtcggaattc tttggagaca tcgagacctg ccgcgccaag gtcccgaaca
     3181 ctgagaccca gctcaacccc gaccatctgc cggtcgacgg catgtttctg accggccaaa
     3241 ccttgcgggt catcagcgag ccgccgccaa ctgggtcgcc aaaaacgacc cctgcgcgga
     3301 cctatatgaa ggcctgggaa aacgcctatg cgtggagcaa cgacagggta ctccagggag
     3361 acgtcatcac ctatgattcc tataaagatt taatttatgc ctatggcgaa aacggtcgca
     3421 aggtgatctt tgccgagcaa tacgctcttg gtcagccgac ctcgcccggc gcctcgagtg
     3481 ccgttcagtt gaaccccaag accggcacga tccatttgat caacagtgat acgctgcaga
     3541 tgctcgataa aaaaaccggg agccggcccg gcatggccgg gcagatcgat cccaaccccg
     3601 tgaagccaaa gaagaccaag agaccgttca agcagcccac acagagccag gaacgtaagg
     3661 gcttcaccgg aacctgacgc atcggagatg cctcgcattg tggtccgcgg cgggctgtgt
     3721 gagatacgtt cggcgcgcgg aattcgagcg cgacaggtgg gccgaacggt gcataccgcg
     3781 acctgagcta gcaacgactt gacccatggc ccgagcgcga ggatgactgg tgattcactg
     3841 tgccgcgacc atgctaaggg cgctcgtggt tgatgatccg gctcaactgg tcgatcgtga
     3901 tgttgccgcc gctcacgatc aggccgacgt tcttgccggc gagctggtcg cgccgcttca
     3961 tggcggcggc gagggccgcc gcgccggctc cctcgacgac ctggcgagcc gtcgtgacca
     4021 gcaggcggat cgcggccgcg atctcgtcat cgctgacgag cacgatctca tcaaccaagc
     4081 gagggaacag ggcaagcggc atggcaaagg gctcgcgcgt ggccagcccc tcggcgaacg
     4141 tattcacgct atcggtaacg acccgccgcc cctcgcgcca cgagtggtaa accgcgggcg
     4201 ccccctcggc ttgcacgccc acgacccgaa ttgccgggtt cacagctcga gccacaatcc
     4261 ccgcgcccag tatgccgcta ccgccgccca cgggcacaaa aagcacgtcg acatcgggag
     4321 ccgcctcgag cagttcgagc gcggcggtgc cgacacctgc gaccaggaga ggttcgttgg
     4381 ccgagtggac gtagcgcgaa aggtagcgcg ctgcgaattc ctccgcggcg agccgcgcgc
     4441 tatcgaaatc ccgaccggtc tggatcacct cggcccccaa ggccacagtc gccgccactt
     4501 tgagcgggtt ggcagcttcg ggcatgaaga tcgttgcctt gcagccgaac agtcgcgccg
     4561 cgtaggcaac ggattggccg tggtttcccg tcgaggccgc gacgactcct cgagaacgaa
     4621 cgtcgggatc gagcgcagcc accaggttga caccgccgcg caccttgaag gcaccggtcg
     4681 ggttcaggtt ctcgcatttg atcacggcac ggcagcccag ggccaaggcg aggcccgaag
     4741 gttccagcga tggtgtcact ggcaggtagg gcgcgatcaa tcgtcgcgcc ttgagaacat
     4801 cggcgagagt cggactcggc caatccatcg ggtctatcac ggacaagttc gaccagggag
     4861 tatcacgacg ccaaaacgaa tcgagctgtg gaccatgcaa accaggttac cgcttcagag
     4921 cgaaacggtc gagctcatcg acctgaccaa gcaaaggtcg tcaacggctc tctgcgaccc
     4981 ggtgtgcggg acgcgtggct ctggcgcgta taaagccggc tccagccaag gtcgtccaga
     5041 gcaagagcga caacggttcg ggggtcgcgg tcgcgggggt gcctgaagta gacgacgaag
     5101 aggaggagga cgaggacgag gaggacgagg acgaggacga ggacgaggac gaggaggacg
     5161 aggacgagga cgagttcgtg gagtccaggg taatcgacac gccaggggtc tgcgactcga
     5221 gcttgggagg gtcgctggca ttagtgacat tgagcgagaa gttcagaacg ccgccggcgg
     5281 cgagcccttg gccgaagaag ctcaggccga gtgcctgcag cggcttgcca ttctggtccg
     5341 tcgtgctcaa aaggaagtca aatacgccag cgctactaaa gccctgcgag cccggcatga
     5401 ttgtcagcgg gcccttcgtg gacgttgcgg gcggggtgac gacgccgccc gcggggtcga
     5461 tgagtgccac cacttgcggc gttgtgacgc ttgttgcgcc ggcagtgggg gggggaccgg
     5521 tgggagcagc gatactgctg gtgctcgtca aggtatagtc ggccgtaccg gcttggtcgg
     5581 cctgcgcgaa cggcgattga gaacccaagc caaaagcgag ggcagccaac agtgcaatca
     5641 tgggagcttt acaacgacgt atcacggcaa gtctcctcct tgaactctca aacttcgatc
     5701 tggttcatcc ttgagccaat ccgtcgcacc catcacccct cttgagcgtc tcgcgccaca
     5761 tgggcctgac gcggccgtgg cggaatccga cggaatgaag ctccagcagt tccattggga
     5821 tgccgatcca atccggaatg aaaggcgaag gcatacgctc tggtcccagc cgcagtccga
     5881 cccggtctga ctcattaggg gagtcaatga ccacctgccg cgaacctcag gatgctcgga
     5941 gggcgttgat cgacgaccga gacgatcatc accgtcagtt gtgctcgtgc atcgcaaacg
     6001 tttgccgtaa ccagcacgac tcactcaagc cgcggaagac tcgcagcaga ggtgtaagtt
     6061 caatgaaagc tcgatctccg aaaacttgag ggccgcattg tgcaagagtt cgcaaaaagg
     6121 gtcaagccgg aatggcatga attccgagtc cccagtctga tcgcgctaat gacagatgga
     6181 acttgagcgg tatcccatta taggcaaaag ctttaggact ggcgacttac gaagcttcaa
     6241 taacttggat cgcgggccac ccatggagtg gaccgggcgt caaagagcac cattgcccac
     6301 gaaacggtcg gaaaatgtga aacagaaacg agcttcactg ggcagggcat aagcggacgg
     6361 ataggcttga tggagaaggg taaaatgagg ccagactgag cgcagagcga ataaggtaaa
     6421 ggcgttccgc gtctacctac ataaggtgag tgtatggtcc agaccctcag cgacgtgcag
     6481 acgcccgacg atacgctggt gaggcgcgct cgcgcggggc agagttcggc ccgcgaagag
     6541 ctgtttcatc gtcatcgagg cgaatcgtac cgatacgcgt accgtctgct ggggaacgaa
     6601 caagatgctc tcgatgtggt tcaggaggcg atgctcaagg cgttttcggc gctggcggat
     6661 ttcgacgggc gtagcggatt ccggacctgg ctgctgcgga tcgtcaccaa cacggcgtac
     6721 gactggggcc ggcggcgcaa gcggcggcca agcgcccgcg gcggagatga agcgacaagc
     6781 tacgaaccta caagtgacga ggatccggct caccgcctca atcagcaaga actgcgtcgt
     6841 gagctcgatc aagcactcaa tcgcctcagc tcgacactca gggaaacatt tattctgtat
     6901 gccgagttag gcctcagcta caaggaaatt gccgagaccc aggatgtccc gatcggtacc
     6961 gtgatgagcc gaattcatgc cgcccgcgaa aagttgcagg ccggactcga ttgggacaag
     7021 ctgagcggtc tctgatgtag ataaagagga tcgtttccaa tgcaatgcga taaagtgatc
     7081 cgcgagctgg ctgtgccaac ggacgaccgt gatccggcca gtctggccga gcacttggcg
     7141 gactgtccgt catgcgccgt ctgggccgac cgcgccgcac ggttcgatcg tctctgggaa
     7201 gcgaccagac cggcggaacc caacggtgag atgtgggata cgctctggac tcaggtgtct
     7261 cgtgcggtcg acgaatcgac gccagtagag caaaatgcgt tcacgttgca cccatcgtct
     7321 ttgaacggat cggcggccaa gaccgaaaaa tcgcccgctc ctaagggccc ctcgtctggt
     7381 ttccgcgcgg ggaggcttat ggcgatcggt tcactcgg
//
LOCUS       DATAQL010000466         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2184850, whole genome shotgun sequence.
ACCESSION   DATAQL010000466 DATAQL010000000
VERSION     DATAQL010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184850"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..>1648
                     /gene="valS"
                     /locus_tag="VHS97_08085"
     CDS             158..>1648
                     /gene="valS"
                     /locus_tag="VHS97_08085"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX3448200.1"
                     /translation="MTELELPKQYDPKEAQNRFYEFWVEKGYFHADPASAQPAYCIVI
                     PPPNVTGALHLGHALNNTLQDILIRWRRMQGYDVLWMPGTDHAGIATQAVVEKRLLEE
                     EKKTRHDLGREALVERIWTWKDEYEKRILSQLRLMGCSCDWERTRFTLDELCSRAVRR
                     TFFNLFKAGKIFRGKRLVNWDTQLRTAVADDEIYYEETAGHLWTIRYPIAGSAAGEVL
                     HIATTRPETMLGDTAVAVHPDDARYKHLIGRQVELPLTGRTIPIIADALLVDPAFGTG
                     VVKVTPGHDPNDYQAGLRNGLPMINLLNPDGTYNENAGKYAGIDRLRARKRVVEDLKA
                     LDLLERVDSYLVRLNHSDRSKTPIEPYLSDQWFVRMGDEPTGAPGFAQQAMDAVTSGR
                     VRIHPERYAKSYLDWLVEKRDWCISRQLWWGHRIPIWYCGTCAEQDLARAFAGRDDVV
                     WRQAEAGGWLVCAESDLRGDELGASHTLIQDADVLDTWFSSALWPHS"
BASE COUNT          327 a          502 c          507 g          312 t
ORIGIN      
        1 gcccaccttc gcgggattgc ctgatcgtct tgtcggttcg ctacggagcc gatgaagaat
       61 cattgtacat cgttgactgc gaccggctgt tggacgataa acccgactga aagcacgacg
      121 caatgcggca accgttgtga aatcaagcga tccgaccatg accgaacttg agctccccaa
      181 acaatacgac cccaaggagg ctcagaaccg cttttacgag ttctgggtcg aaaagggcta
      241 ttttcacgcc gatccggcca gcgctcagcc ggcctactgc atcgtgattc cgccaccgaa
      301 cgtcaccggt gctctccacc tggggcacgc gctcaataat acgctgcaag acatcttgat
      361 ccgttggcgg cggatgcagg gctatgacgt cctttggatg cccggcaccg accacgcggg
      421 tatcgccact caggcggttg tcgaaaagag gctgctggaa gaggagaaaa agacacggca
      481 cgacctgggc cgcgaggcct tggtcgagcg aatctggacc tggaaagacg agtacgaaaa
      541 gcgaatcctg agccagctcc gcttgatggg atgttcctgc gattgggagc ggacgcggtt
      601 taccttggac gaactgtgct cgcgcgccgt ccggcgcacg tttttcaacc tcttcaaggc
      661 ggggaagatc tttcgaggca agcggctggt caactgggat acccagctcc gcaccgcggt
      721 ggccgacgac gagatttact acgaagaaac cgccggccat ctttggacga tacgatatcc
      781 gatcgcgggc tcggcagccg gcgaggtcct tcacatcgcc accacccgtc ccgagaccat
      841 gctgggggac acggctgttg ccgttcatcc cgacgatgcg cggtacaagc atttgatcgg
      901 ccggcaggtg gagttgcccc ttaccggccg cacgattccg atcatcgccg atgcgcttct
      961 ggttgacccg gctttcggca ccggtgtcgt caaggtcacc cccggacacg accccaacga
     1021 ttatcaggcc gggctgcgca acgggttgcc tatgatcaat ctgctcaacc cggacgggac
     1081 gtacaacgaa aacgccggca aatacgccgg catcgatcgc ctgcgggcgc gaaagcgggt
     1141 cgttgaggac ttgaaggcac tcgatttgct cgaacgggtc gattcgtacc tcgtgcgcct
     1201 taaccactcc gaccggagca agacgccgat cgagccgtat ctctccgacc agtggttcgt
     1261 ccgcatggga gacgagccga ccggtgctcc tggctttgca cagcaggcga tggacgcggt
     1321 gacctcgggc cgagtgcgga ttcatcccga gcgctacgcc aagagctatc tcgactggct
     1381 cgtggagaag cgcgattggt gtatcagccg ccagctttgg tggggacatc gaatccccat
     1441 ctggtattgc ggtacctgtg ccgagcaaga tcttgcccgc gccttcgccg gccgcgatga
     1501 cgtggtctgg cggcaggcag aggcgggtgg atggctcgtc tgcgccgagt ccgatttgcg
     1561 cggcgacgag ctgggggcga gccataccct gatccaagac gcggacgttc tcgatacgtg
     1621 gttcagctcg gccttgtggc ctcactcg
//
LOCUS       DATAQL010000467         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2337769, whole genome shotgun sequence.
ACCESSION   DATAQL010000467 DATAQL010000000
VERSION     DATAQL010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337769"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2697)
                     /locus_tag="VHS97_08090"
     CDS             complement(<1..2697)
                     /locus_tag="VHS97_08090"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3448201.1"
                     /translation="MQRTPTRTSLNCVCWVIGLTAGLAAGPVQTQALAKEPGSDIRPI
                     LSQYCFECHGDDLAEGRINLEEMTGASDFGRRFKDWEKVNRVLRDRKMPPKKMPQPSD
                     SERKGVIQTVADELNRFIEQHAGDPGRVVIRRLTSAEYAYTIQDLTGLELNALDGLVG
                     DAASGEGFTNAGAGQFMQDSTLERYLEMAKIVADHAVIGAGPLEFYADPGQTGRELSA
                     IRRIQTIYRAHGFRTAAGEGAKPFGLDLYPRAMLVAWQYRFRGELGLANDTLPQLARR
                     EGLSVQLCEHVWSVVNEQHTSFPLSLIISAWQSLPPPAQQSAEITRKRCLELCDNLRE
                     WQKTLAAAAGDEEEAAVLTAGDVHVATTHKLTADLVWPKGSTVTKLDFAVSRASKHPA
                     ASAVVVWQNARLRFRRENGRREPYQPLTAFVNPESARRLDFGKHPQGTAIGETDFVIA
                     GEVIVPVSVQIPNGMVSAQLLVDVRLDTKQDETSIVRCRISHGEVKGEISTAFSATST
                     LLADPANKLVDEWRHDVAEFARLLPEVSHREPAPSDRDPIPNPYDNTYNKPERNHFHS
                     IIKYNRDDDFFVAHIADDTTQRRLDLAWTDLLTSFEYHDANLRFVAKKFALDLGGRSI
                     ADLDRTLIDRLPAAPRVFVKLWVDEYTSMQNALRTAEPGHVADALTFAERAWRRPLTA
                     DEEKRLRAFYAGLRQQNNMDHPQALRALMARILVAPTFLYRVEPPRERQAIVPLSDRQ
                     LANRLSYFVWSSVPDQELRQAADLERLHEPRQLEQQTRRMLHHPKVRRLATEFFGQWL
                     GFYRFDGYQGIDAKRFPEFTDLLKSSLYEESVLFFEHIVRDDRPVDEILFADYAFWNR
                     RLAEHYGMKSSNLSNETFARVDGITQHHRGGLLGLGAVLAVTS"
BASE COUNT          560 a          861 c          878 g          580 t
ORIGIN      
        1 cgaggtcacc gcgagcacgg cgcccaatcc cagaagaccg ccgcggtgat gttgagtgat
       61 gccgtcgacc cgcgcaaagg tctcattcga gagattactg gacttcatgc cgtagtgctc
      121 ggcaaggcgc ctattccaga acgcgtaatc cgcaaacagg atctcgtcaa caggccggtc
      181 gtcgcggacg atatgttcaa agaataacac tgattcctcg tagagcgagg atttgagcag
      241 atcggtgaac tcggggaagc gcttggcatc gattccttga tagccgtcga agcggtaaaa
      301 ccccagccac tgaccgaaga attccgtcgc gagccggcgg actttcgggt ggtgcagcat
      361 gcggcgcgtt tgttgctcga gctgacgggg ctcgtgcaag cgttccaggt cagcggcctg
      421 ccgaagctct tgatcaggca ccgacgacca cacgaaatag ctgagtcgat tggcgagctg
      481 gcgatcggaa agcggcacga tggcttgccg ttcacgcggc ggttcgacgc ggtaaaggaa
      541 tgtcggcgcg accaggattc gggccatcag tgcgcggagg gcctgtggat gatccatgtt
      601 gttttgttga cgcagcccgg cataaaacgc ccgcagacgc ttctcctcgt ccgcggtcaa
      661 cggcctgcgc caggctcgct cggcaaacgt cagggcgtct gcaacatgac ccggctcggc
      721 ggtccgaagc gcgttctgca tcgacgtata ttcgtcaacc cagagtttga cgaatactcg
      781 gggggcggcc ggtaatcgat cgatcagcgt gcggtcgaga tccgcaatcg accggccgcc
      841 gagatcgagc gcgaacttct tggcgacgaa tcgcaggttc gcgtcatgat actcaaacga
      901 cgtgaggagg tcggtccagg ccagatccaa ccgccgctgc gtcgtgtcat cggcaatgtg
      961 cgcgacaaag aagtcatcgt cacggttgta tttgattatg ctatgaaagt gattgcgttc
     1021 tggtttatta taggtgttgt cataaggatt cggaatcggg tcacggtcgg aaggcgccgg
     1081 ctcgcggtgc gagacttcgg ggagcagccg cgcaaactcg gcgacatcgt gtcgccactc
     1141 gtcgaccagc ttattcgccg ggtccgccag cagggtcgag gtcgcgctaa acgcggtgct
     1201 aatctcacct ttcacttcac cgtgtgaaat gcggcaacga acgatgctcg tctcatcttg
     1261 ctttgtgtcc agcctgacgt cgaccaaaag ctgtgccgac accatgccgt tggggatctg
     1321 gaccgagacg gggacgatca cttcgccggc gatcacaaag tcagtctcgc cgatggccgt
     1381 accctgcgga tgtttcccga aatcaagccg tcgtgccgac tcggggttca cgaaggctgt
     1441 gagcggttgg tacggttctc ggcgtccgtt ctcgcgccga aatcgcaacc gtgcgttctg
     1501 ccagacgacc actgcactgg ctgccgggtg cttgctggcg cgcgagacgg cgaagtcgag
     1561 cttggtaacc gttgaaccct ttggccacac gagatcagcc gtcaacttgt gggtcgtggc
     1621 aacgtggacg tcgcccgcag tgagcacggc ggcctcttcc tcgtcgccgg cggccgcagc
     1681 gagcgttttc tgccattctc ggaggttgtc gcacagctcc aggcatcgct tgcgggttat
     1741 ctccgccgac tgctgcgcag ggggcggaag cgattgccat gccgagatga tgagtgataa
     1801 cggaaacgac gtgtgctgct cgttcacgac gctccacacg tgctcacata actggacact
     1861 cagaccctcg cgccgagcga gttggggcaa ggtgtcgttc gccagaccga gttctccgcg
     1921 gaaacggtat tgccacgcca ccaacatcgc cctgggatag agatccaggc caaacggctt
     1981 ggcgccttct ccggctgcgg tgcggaaacc gtgcgcacgg tagatcgttt gaatgcgcct
     2041 gatcgccgac aactcacgcc ctgtctgacc tgggtccgca tagaattcaa gcggccccgc
     2101 gccgatcacg gcatggtcgg cgacgatttt ggccatttcg aggtagcgct cgagagtcga
     2161 atcttgcatg aactgccctg cgccggcgtt ggtgaatccc tcaccagagg cggcgtcgcc
     2221 aacaagaccg tcaagcgcgt tgagctcgag cccggtcagg tcctggatcg tgtacgcata
     2281 ctctgcgctc gtgagccgcc ggatcacaac tcgcccaggg tcgccggcat gttgctcgat
     2341 aaatcgattg agctcatcgg caacagtctg aatgactccc ttccgctctg agtcacttgg
     2401 ctgtggcatc ttcttgggcg gcatcttccg gtcgcgtagc acgcgattga ccttctccca
     2461 atccttgaaa cgccggccga aatccgaggc ccccgtcatc tcttcaaggt tgattcgtcc
     2521 ctcggccaga tcgtcgccat gacattcaaa gcaatactgg ctgaggattg gtcgaatgtc
     2581 cgacccaggc tccttcgcca gcgcctgagt ttgaaccggt cccgccgcca agccggcggt
     2641 gagaccaatc acccagcaga cgcagttcaa cgaagttcga gttggagtac gttgcacgcg
     2701 atcacctctg tcacgacacg aggatggtcc gactcggtct gccgccgcac gtacgcgacc
     2761 tgtttcctgc acgatactct tcggcggcgg tgatgcaaag cgggtctgtc gaatcgccaa
     2821 cgtgccaaga agcaggtcgc tgtgctcgat cgggtgaatt cagctagcag cggcacgcg
//
LOCUS       DATAQL010000468         4035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_808886, whole genome shotgun sequence.
ACCESSION   DATAQL010000468 DATAQL010000000
VERSION     DATAQL010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808886"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="cysK"
                     /locus_tag="VHS97_08095"
     CDS             complement(<1..851)
                     /gene="cysK"
                     /locus_tag="VHS97_08095"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX3448202.1"
                     /translation="MPTAEPSFLRGRIYDDITQTIGNTPLIRLRRITSGCHADVVAKL
                     ENFNPLWSVKDRIGVAMINAAEEQGKIKKDTVIIEPTSGNTGIALAFACAARGYRLIV
                     TMPESMSLERRRLLKAFGAEIVLTPAAEGMPGAVRKAEELVKNTPGSFMPQQFKNAAN
                     PEVHRRTTAEEIWRDTAGKIDFLVSGVGTGGTITGCGEVLKSRKPSVQIIAVEPVNSP
                     VITQLRHKEALRPGPHKIQGIGAGFIPDVLNISIIDDVLTVRDEDAFETARRLAREEG
                     MLCGISCG"
     gene            complement(1068..2186)
                     /locus_tag="VHS97_08100"
     CDS             complement(1068..2186)
                     /locus_tag="VHS97_08100"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX3448203.1"
                     /translation="MNTWNRVRTLAVLAIGMLTGAGLAAAWGDRGKAVAAPASNQPSG
                     AGESKASLTQSDKIPGWEKGKGWGWIWGKDDEVGSLNALSNRSRAAALAMATRGEVFD
                     LGLTYSRRSFKYSGHSPGEIITFRSPDGVHRMKDADAPPAAMNADLVYWHSAALFISD
                     NVATQIDGLAHITAGPDFHWYNGFKEGDWGGDWGPRKCDAPTIPPIIARGVLIDVAAY
                     KRVEALPGHTAILTKDLQETLAWEGVSLAAGDVVLVRTGTGRFWGDDGADHARIANHD
                     SAGPDLEATRWLVEEQGAIMVGSDTSGFEVNPPTGSPGSAIPVHRYLLVDQGVHLGEF
                     HYLEKLSQARAYTFCYVATVNKIKGAAAGFALRPVALR"
     gene            2396..3322
                     /gene="hemQ"
                     /locus_tag="VHS97_08105"
     CDS             2396..3322
                     /gene="hemQ"
                     /locus_tag="VHS97_08105"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX3448204.1"
                     /translation="MPQDQSGPSPSPSEAGTARGEIKTHPMQHGHRPAVSEQVPATLV
                     PEKGWHFLHVFYRVDRARLGELSAENRQRGVEDFRRVLGAKTPGAPEQLQCFAVPGHK
                     ADFGIVMAGPDLRAIHDVQMAVQASSLGPALQPTYSFYSITEISEYVPDADDYARILR
                     EREGLDPDSSMFKAKVGAYAERLGPMNRQRLFPDFPNWPCLCFYPMSKMRSGDQNWYL
                     LPFETRTELMAQHGKSGMKFAGKVSQVITASTGLDNWEWGVTLWARNPSYLKDIVYSM
                     RFDESSAKYALFGEFYFGYILPPDELLETIRL"
     gene            3452..3949
                     /locus_tag="VHS97_08110"
     CDS             3452..3949
                     /locus_tag="VHS97_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated DNA-protein cysteine
                     methyltransferase"
                     /protein_id="HEX3448205.1"
                     /translation="MSKQATKTRSSWSEKLDQSKGLPKVVPIGPTMSKQWGEGTVVVP
                     TPREVDEIMKAVPAGKLITIQEIRAILAERHNATIGCPLTVGIFAWIAAHAAEEDHKS
                     KRRGATPYWRTLKSGGFLNEKFPGGVTAHRAKLEAEGHDIVQRGQKLAVVDYKDFLVD
                     PATVE"
BASE COUNT          787 a         1284 c         1187 g          777 t
ORIGIN      
        1 ccgcaggaaa tgccgcagag catcccctcc tcacgagcca gccggcgggc tgtctcgaag
       61 gcatcttcgt cgcggaccgt caacacatca tcaataatag atatgttgag cacgtccggg
      121 ataaacccgg cgccgatgcc ctggatcttg tgcgggccgg gccgcagcgc ttccttgtgc
      181 cgcaattgag tgatcaccgg tgagttgacc ggctcgaccg cgatgatctg taccgatggc
      241 ttgcggcttt tgagcacctc gccgcaaccg gtgatcgttc cgcccgtgcc gacaccgcta
      301 accaagaagt cgatcttgcc agccgtgtcg cgccagattt cttcggccgt cgtgcgccga
      361 tgcacctccg ggttggcggc gtttttgaac tgctggggca tgaacgatcc cggcgtgttc
      421 ttgacgagtt cttcggcctt gcgcaccgca cccggcattc cctcggcggc cggcgtcagc
      481 acgatctcgg ctccaaacgc cttgagcaga cgccggcgct cgaggctcat gctctcgggc
      541 atcgtcacga tcagccggta accccgcgcc gcacatgcga acgccagcgc gatacccgtg
      601 ttgccgctgg tcggctcaat gatcactgtg tctttcttga tcttcccctg ttcctcagcc
      661 gcgttgatca tcgccacacc gatccgatcc ttgaccgacc agagtgggtt gaaattctcg
      721 agtttggcga ccacgtcggc atgacacccg ctcgtgatcc gccgcagacg gatcaagggt
      781 gtattcccga tcgtttgagt gatgtcgtcg taaatccgtc cgcgcaggaa actcggttcg
      841 gccgtcggca cgactcgcgc cctccccaaa ctcggactca atgcaccggt catcctcgca
      901 cggcccgcct aacgtgtttt gcggaccacc accgccggga tcgacccatc ccgaatgcga
      961 gcaaaaccag tgatatttcg gtgcaagaca ctcgttgcaa cagattcccg atcatagtag
     1021 tccacaagaa tcgcggcagc aaccctgatc ttgtcgccaa tcaagcgtca tcgcagtgcc
     1081 acaggacgca aggcgaagcc ggcggcggcg cctttgatct tgttgaccgt tgcgacgtag
     1141 caaaaggtgt aggcccgcgc ctggctcaac ttttccaggt aatgaaactc tccgagatga
     1201 acaccctgat caacgagcaa gtagcgatgg acgggaatgg ccgatcccgg cgagccggtg
     1261 ggtgggttga cctcaaaccc gctcgtgtcg gacccgacca tgatcgctcc ttgctcttca
     1321 accagccacc gggtcgcttc aagatcgggg ccagccgagt cgtggttggc aatccgggca
     1381 tggtcggcac cgtcatcacc ccagaagcgg ccggtgcccg tgcgcactag caccacatcg
     1441 cccgccgcga ggctcacccc ctcccaggcc agtgtttcct gcaggtcctt ggtcaatatc
     1501 gcggtatggc cgggaagggc ctcgacgcgc ttgtaggcgg ccacgtcgat gagcacgcca
     1561 cgagcgatga tcggcggaat ggtcggtgcg tcgcatttgc gcgggcccca atcgccgccc
     1621 caatcaccct ccttaaagcc gttgtaccag tgaaagtccg gacctgccgt gatgtgagcc
     1681 agcccgtcga tctgggtcgc cacgttgtcg gagatgaata acgcggccga atgccaatag
     1741 acaagatccg cgttcattgc cgcgggaggc gcgtccgcgt ctttcatccg atgtacgccg
     1801 tcggggctgc gaaaagtgat gatctcgccg gggctgtggc ccgaatactt gaagctgcgg
     1861 cggctgtagg tcagccccag atcaaatacc tcgccgcgcg tcgccatcgc cagcgccgcc
     1921 gcgcgcgagc ggtttgaaag cgcgttgagt gatcctacct cgtcgtcttt gccccagatc
     1981 cagccccatc ccttcccctt ctcccacccg ggtatcttgt cagattgggt taggctggcc
     2041 ttggactcgc cggccccgct tggttggttg ctagccggcg cggctacggc tttccctcgg
     2101 tcgccccagg ccgcagccaa acccgcgccc gtaagcatgc cgatcgccaa aaccgcgagc
     2161 gtccgcaccc gattccacgt attcatgttg tcccactccg tcgtatgccc actgtctacc
     2221 gatgatccgc caggccagcc gcgagggcat tctaggccgc gcagggcggc gctgcaatcg
     2281 tcttgagaat gactgagcct tgaccgcgct tgccgtcccc catcccgaag tcgtacaatg
     2341 aattggaatg aatcagccgt cggagtctgg gccaaagaga gcaggagcgc tcgccatgcc
     2401 gcaagaccaa tcaggaccat cgccttcgcc gtcggaagca ggaactgcgc gcggcgagat
     2461 caagacgcac ccgatgcagc atggtcaccg gccggccgta tcggaacagg tccccgcgac
     2521 actggttccc gagaagggct ggcactttct ccacgtgttc tacagggtcg accgcgcgcg
     2581 gctgggcgag ttgtcggccg aaaaccgcca gcgcggcgtt gaagacttcc ggcgggtctt
     2641 gggagccaag accccgggtg ctcccgagca attgcagtgc ttcgccgtcc caggccataa
     2701 ggcggatttt ggaattgtca tggccgggcc cgaccttcgt gcgattcacg acgtccagat
     2761 ggccgtccag gcctcgagcc tgggccccgc gctccaacca acttactcgt tctactcgat
     2821 cacggagatt tccgagtacg tgcccgacgc cgatgactat gcgcgaatcc tccgtgagcg
     2881 cgagggactc gatcccgaca gcagcatgtt caaagccaag gtcggcgcct atgccgaacg
     2941 gctaggacca atgaacaggc agcggctctt tcccgatttt cctaactggc cgtgcctctg
     3001 cttttatccg atgagcaaga tgcgctcggg cgatcaaaac tggtacttgc tcccttttga
     3061 aacgcgaacc gagctcatgg cgcagcacgg caagagcggg atgaaatttg cgggtaaagt
     3121 atcgcaagtc atcacggctt ccactggtct cgacaactgg gaatggggtg tcacgctgtg
     3181 ggcgcgcaac ccttcctatc tgaaagacat cgtctactcg atgcggtttg acgagagttc
     3241 ggcgaagtat gcacttttcg gcgaattcta cttcggctac atcctgcccc ccgacgagtt
     3301 gctggaaacg atccggctat agttggccgc cgatcgttca caaggaatgg attggaatgg
     3361 agtggcgcgg tcccggcact ggacgtcacg ttcccggtcg ttccccttga tcgatatcga
     3421 tactcaaaaa gccaaaggaa ctgaacgagc gatgagtaaa caagcgacga aaacgcgatc
     3481 ttcttggagt gaaaagctgg accaaagtaa aggtttaccc aaggtcgtgc cgatcggtcc
     3541 gacgatgagc aagcaatggg gcgaaggaac ggtcgtggtg ccaaccccgc gcgaagtcga
     3601 tgagatcatg aaggcagtcc ctgcgggcaa gttgatcacg atccaggaga ttcgcgcgat
     3661 cttggccgag cgccacaacg ccacgatcgg ctgtccgctc acagtcggca ttttcgcatg
     3721 gatcgcggcg catgccgccg aagaagacca caagtccaaa cgccgcggcg ccacccctta
     3781 ctggcggacg ctcaagtcgg ggggcttttt gaatgagaag tttcccggcg gcgtgacggc
     3841 ccatcgagcg aagctcgagg ccgaaggtca cgacatcgtg cagcgcggcc agaaattggc
     3901 ggtagtcgac tacaaggact ttctggtcga tccggccacg gtcgagtgat gcagggccgt
     3961 gacgctggcg atcgttcagc cacggatcct cagcccgagc tggtgctggc gagcacatcg
     4021 gtttacaggc gggcc
//
LOCUS       DATAQL010000469         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_351712, whole genome shotgun sequence.
ACCESSION   DATAQL010000469 DATAQL010000000
VERSION     DATAQL010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351712"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..622)
                     /locus_tag="VHS97_08115"
     CDS             complement(260..622)
                     /locus_tag="VHS97_08115"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX3448206.1"
                     /translation="MATPTSQELTSKRCVPCEGGIPALSAQEALALAQNVTGWIVDSA
                     AKQISRSWTVKNFMAGIDFFNKVAALAEEEGHHPDLHLEGYRQVKIVLSTHAVGGLTE
                     NDFILAAKINELPIQVRR"
     gene            complement(662..1462)
                     /gene="panB"
                     /locus_tag="VHS97_08120"
     CDS             complement(662..1462)
                     /gene="panB"
                     /locus_tag="VHS97_08120"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX3448207.1"
                     /translation="MPTDVQPVTIPEFLAWKSQHRRISMLTAYDFPTARLLDAAGVDC
                     LLVGDSLGTVVQGWETTLRVTIDQIIYHAEMVSRAARRALVVADLPFLSYQVSPRQAL
                     KSAGRILKETNCQAVKLEGGRKMAATIKAIVDADIPVMGHVGLRPQSIRRIGTYKVQR
                     STDEIVDDAAAVAESGAFAVVLECIPRELAAKITAQVEIPTIGIGAGPFCDGQVLVFH
                     DLLGLFEGHRPKFVRQYADLGHQIKAAVAGYIGDVVEGRFPGETESFR"
     gene            complement(1452..2969)
                     /locus_tag="VHS97_08125"
     CDS             complement(1452..2969)
                     /locus_tag="VHS97_08125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3448208.1"
                     /translation="MDAGDERSGSRAYWWNCRSSLSLLLLATFVIRVLYAGQPIVENY
                     VGRQIPTAMVARNLDRGSGFLRPTLDTAPFPNYFVVEPPIYESGVVALKRATGLGLDE
                     AGRIFSALATALAAWGLYMLARRREGTLVALAAVAAFAVFPLTIRYGRAFQPDALMLG
                     AVVAGLASWDEYRSVGGWYWLVAGWSLLSLGFATKITAAFLLIPLLLFVARTRSPREL
                     LLVCSTLLPAALWYVWASYLLALGEGSRASADNRLIWLGHFGPVALLEPETLKFVSWC
                     LLVRAFTPVGAGLALVGMFYGGAARGRRDALWLVWGISAMLALAFLSRKLHHEYYWLL
                     IAPVVAVGIGRTVVLFAQNRRAGAATLSAAFVVLSWVQVRSTWRTPPEWSGLEPAADA
                     VRATVPSDAWAVAPEALLFQADRRGCRLEYTLPAAARAAGEWGKESSVEGPHDLIETY
                     RHQGARYFADLGSRADDPRRKGLHDFVRQRYKVIVDGPEVIIAELADSEMHWNAN"
     gene            3258..3692
                     /locus_tag="VHS97_08130"
     CDS             3258..3692
                     /locus_tag="VHS97_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3448209.1"
                     /translation="MLVQDVLVSKGRRVVAIDSEATVSEAVAKLVQNNIGSLPVIDHV
                     GHLIGMFSERDVLRVIHGRGEGFGRLRVADVMTCNPVTCKLDDDVNEVMGKMSERHIA
                     KVPVLSDSELVGIVSVGDVIKVLYDQVHTENQHLISYIHGAV"
     gene            complement(3677..5296)
                     /gene="malQ"
                     /locus_tag="VHS97_08135"
     CDS             complement(3677..5296)
                     /gene="malQ"
                     /locus_tag="VHS97_08135"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX3448210.1"
                     /translation="MRLPRAAGVLLHPTSLPGRHGIGDLGAEAHAFVDFLAATGQRWW
                     QILPLGPTGYGNSPYQSTSSFAGNHLLINIDDLARRGWLTPEAVLREARSPSDHVDFD
                     TASVLKAGSLRLAYEGFKAQDDDPKFEEFIAANSGWLDDFVFYQALKDVHVGRPWYQW
                     EPELVEREPAAMAAWRARLTEGIRYHEFVQYAFEIQWQELRADCQRQGLMLIGDLPIF
                     VAHDSADVWANPELFYLDKGGQPLIVAGVPPDYFSETGQLWGNPLYRWDAHAADDYAW
                     WAARLRFLLGRVDIVRIDHFRGFEAYWEIPAGSTTAASGKWVEGPASKFFDAMRRRLG
                     SLPLIAEDLGVITPGVEALRDEFSLPGMRVLQFGFGSDSGLEKHLPHRFLPHCVVYTG
                     THDNDTTKGWFTSTDVATTQTWEEIQAERAYARRYIDTDGDEIHWDLIRLAFSSVADT
                     AIIPLQDILGLDSRARMNLPGKAEGNWLWRFQHGQLDQEAQDRYAELTALYSRWNGAL
                     PAEWNPRCRPQNLGHSPAAESVAANVTQTAP"
     gene            complement(5350..>6085)
                     /locus_tag="VHS97_08140"
     CDS             complement(5350..>6085)
                     /locus_tag="VHS97_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3448211.1"
                     /translation="VFLSGLGGDNRAFSRALRHFGTRFRALAFDSRDAGQSDRSDRAY
                     TTADMADDVAGWLGAIGAGPAHVVGQSLGGLVAQELALRHPQLVQSLVLVSTHAGGDP
                     WRQAVIESWVLVRRMVDAGAFTRAVLPWLVAPAFYRQPAQVEGLVQFAERNPWPQDPE
                     AFARQAQAAATHDTRSRLGQIEVPCLVLVGELDLLNSHRVAAELADKLRNARLVVLPG
                     VGHMPHVEDQTRFRQEIDRFLDQGHA"
BASE COUNT         1218 a         1938 c         1836 g         1093 t
ORIGIN      
        1 gaaggcgaca aagccgcctt ccggcacgga tacgtaaacg gcggtcaact gcatttagac
       61 ctcccatccg cgcgtggcaa tgattcgtcc gcggcgagca ttcaatttat catcgccttg
      121 tcgtgaacgg ccggcaagag cgggtgattc ttcccgacgg agtcgcggcc tcgcctccgc
      181 aggagcgata ccatgactcc cgacacggcc gagcgaaatc aagaccgcca ataccgtgaa
      241 gccgccaaat cgcttcccct cagcgccgga cctgaatcgg tagctcattg atcttggcgg
      301 cgaggatgaa gtcgttttcg gtcaggccgc cgacggcgtg ggtcgagagg acgatcttca
      361 cctggcgata cccttcgaga tgtaggtcgg gatgatgtcc ttcttcctcg gcgagcgcgg
      421 cgaccttgtt gaaaaaatcg atcccggcca tgaagttctt gaccgtccac gagcgcgaga
      481 tctgtttggc ggccgagtcg acgatccatc ccgtgacatt ttgcgccagc gccagagcct
      541 cttgggcgga gagggcgggg atgccccctt cgcagggaac gcaccgtttg ctcgtgagct
      601 cttggctggt gggcgtggcc atgattcggg cctccggggg cagatttctt ttgagttgct
      661 ctcagcgaaa actctcggtt tcaccgggaa agcggccttc gacgacatcc ccgatatagc
      721 cggcgacggc ggccttgatc tggtgaccta agtccgcgta ctgccgcaca aacttgggcc
      781 gatgcccctc gaacaaacct agcaggtcgt ggaaaacgag gacttgcccg tcgcaaaacg
      841 ggccggcacc gatgccgatg gtggggatct ccacctgggc cgtgatcttg gccgccagct
      901 cgcgcggaat gcattcgagc acgaccgcga acgcacccga ctcggcgacc gcggcggcat
      961 cgtcgacgat ctcatcggtc gaccgctgca ccttgtatgt ccctatcctg cggatcgact
     1021 ggggccgcag gccgacgtgg cccatcacgg ggatatccgc gtcgacgatg gctttgatcg
     1081 tggccgccat cttgcggcct ccttccagct tgacggcctg gcaattcgtc tctttaagaa
     1141 ttcgcccagc cgacttgagc gcctgccgtg gcgagacctg atagctcaga aacggcagat
     1201 cagcaacaac gagcgctcgc ctggccgctc gagagaccat ctcggcgtga tagatgatct
     1261 gatcaatcgt cacgcgcaag gttgtttccc aaccctgcac gaccgtgccc agcgagtcac
     1321 cgacaagcag gcaatcgacc cccgcagcgt cgagcagccg ggccgtcgga aagtcgtagg
     1381 ccgtcagcat cgagatccgg cgatgttgcg acttccacgc gagaaattca gggatggtga
     1441 ccggctgaac gtcagttggc attccagtgc atctcggaat cggccagttc ggcgataatg
     1501 acttcgggac cgtccactat gaccttgtat cgttggcgga cgaagtcgtg caagcccttt
     1561 cgccgcgggt cgtcagcgcg gctgcccagg tcggcgaagt aacgagcgcc ttggtgtcga
     1621 taagtttcga tcagatcgtg cgggccttcg accgagcttt cttttcccca ttctccggcc
     1681 gcgcgggccg ccgccggtag agtatactcg aggcggcagc cgcggcgatc cgcctgaaac
     1741 aacaaagcct cgggggcgac cgcccaggca tcggatggaa ccgtcgctcg aacggcgtcg
     1801 gcggccggct ccagaccgct ccattcgggt ggcgttcgcc acgtcgagcg cacctgaacc
     1861 cacgagagga cgacgaatgc tgcagagaga gtcgctgcgc cggcgcggcg gttctgggcg
     1921 aagagaacca cggtacggcc gatccccact gcaacgaccg gcgcgatcaa aagccagtaa
     1981 tattcgtgat gcagtttccg agagaggaac gccagcgcaa gcattgcgga gattccccag
     2041 actagccaaa gcgcatcgcg acgaccgcgt gccgcgcctc cataaaacat tcccaccagc
     2101 gccagtcccg cgcccacggg tgtgaatgcc cggaccagca agcaccaaga cacgaacttc
     2161 agagtctccg gctcgaggag cgcgacgggc ccgaagtgtc ccagccagat caatcggttg
     2221 tcggccgatg ctcgcgagcc ttcgcccaag gcgagcagat agctggccca cacgtaccaa
     2281 agcgcggcgg gcaagagcgt agagcaaacc aagagaagct cgcggggcga gcgggtccgc
     2341 gctacaaaga gcaacagcgg gatgagcaga aacgcggcgg tgatcttggt cgcgaagccc
     2401 agggacaaga gcgaccagcc cgccacgagc caataccatc ccccaactga tcggtattcg
     2461 tcccaagagg ccaatcccgc aacaacggcc cccagcatga gtgcgtcggg ttgaaaggcg
     2521 cgtccatagc ggatcgtaag aggaaagacg gcaaatgcgg ccaccgccgc caaggccaca
     2581 agagtcccct cgcgccgccg cgctagcatg tagagccccc acgcggccag ggccgtcgcc
     2641 agggccgaga agatcctccc ggcctcgtcg aggcccagtc cggtggctcg tttcaaggcc
     2701 accacgcctg actcgtatat gggcggctcg accacgaagt aattgggaaa cggcgccgtg
     2761 tcgagcgtcg gtctcaagaa gcccgacccg cgatcgagat ttcgcgctac catggcggtc
     2821 ggtatctgtc gcccgacata gttttccacg attggctgcc cggcataaag cacacggatt
     2881 acgaacgttg ccagcaagag caagctcaga gacgaacgac aattccacca ataagcgcgc
     2941 gatccagatc gttcgtcccc ggcatccatg cgaagactcc ttgcgccagt caggctgctt
     3001 cacccgctgg ccgagctttc caaacgcggt tttgatcgtc ggttacaatg aatttcttcg
     3061 aatgtaggac atgtttgttc ttttgaagag tgacgtttcc ggaattggtc ggcctgtaaa
     3121 aaaatggttt gcatcgccgg atgaataatg tcacgatgac tcgacgtaac acgtccctta
     3181 ctcaggtcga gagcgtcggt cggtagactc tcgagtcgtt ggctgcgctc gcgtgctcca
     3241 tagctgggag gagcctcatg ctggtacaag acgtccttgt ttccaagggt cgacgcgtcg
     3301 ttgccatcga ttccgaggcg accgtcagcg aagcggttgc caagctcgtc caaaacaaca
     3361 tcggctctct gccggtcatt gatcacgtgg ggcatctcat tgggatgttc agcgagcgtg
     3421 atgtcttgcg cgtcatccac ggccggggcg agggttttgg ccggctgcgc gtggccgacg
     3481 tgatgacctg taatcccgtc acctgcaaac tcgatgatga cgtgaacgaa gtcatgggca
     3541 agatgagcga gcgccacatc gccaaggtcc ccgtgctcag tgattccgag ctcgtcggga
     3601 tcgtctcggt cggcgacgtg atcaaggttc tttacgatca ggttcatact gaaaaccagc
     3661 acttgatctc gtacattcac ggcgcggtct gagtgacatt ggccgcaacg gattcggccg
     3721 ccggggaatg gcccaggttc tgggggcgac accgcgggtt ccactccgcc ggcagcgccc
     3781 cattccatcg gctgtaaagg gcggtcagct cggcgtatcg atcctgggct tcttgatcga
     3841 gctgaccgtg ctgaaaccgc cacagccagt ttccctccgc cttgccaggc aagttcatcc
     3901 gggcacggct gtcgagcccg agtatatctt gcaacgggat gatggccgtg tcggcgaccg
     3961 acgaaaaggc cagccgaatc aggtcccagt gaatctcatc gccgtcggtg tcgatatatc
     4021 ggcgtgcgta tgcgcgctcg gcctggatct cttcccacgt ttgagtcgtc gcgacgtcgg
     4081 tggacgtgaa ccatcctttg gttgtgtcgt tgtcgtgcgt gccggtgtaa accacgcagt
     4141 gcggcaggaa tcggtgcggc agatgctttt ccaaccccga gtcggaaccg aacccaaatt
     4201 ggagtacccg catccccggc agactgaact cgtcgcggag ggcctcgacg ccgggcgtga
     4261 tcacgcccag gtcttcagcg atcaacggga gactgccgag gcgcctccgc atcgcgtcga
     4321 aaaacttgct cgccggccct tcgacccact tgccggacgc tgccgtcgtc gagcctgccg
     4381 gaatctccca atacgcctcg aacccgcgaa aatggtcgat gcggacgatg tcgactcggc
     4441 ccagcaaaaa ccggagccgg gcggcccacc acgcataatc gtccgccgcg tgggcatccc
     4501 aacgatagag cgggttgccc catagctggc cggtctcgct gaaatagtcc ggcggcacgc
     4561 ccgcgacgat cagaggctgc ccccctttgt ccagataaaa gagctcggga tttgcccata
     4621 cgtcggcact atcatgggcg acgaatatcg gcagatcgcc gatcaacatc aacccctgcc
     4681 gctggcagtc cgcccgcagc tcttgccact gaatttcaaa cgcatactgc acgaattcgt
     4741 ggtaacggat accctcggtc agacgcgcgc gccaggcagc catcgccgcc ggctcgcgct
     4801 cgaccaactc gggttcccat tggtaccatg ggcgcccgac gtgcacgtct ttaagtgcct
     4861 ggtaaaagac gaaatcgtcc agccagccgc tattggcggc gatgaattcc tcgaactttg
     4921 ggtcgtcatc ctgcgccttg aaaccctcgt atgccagccg cagcgagcct gcctttaaga
     4981 cgctggcggt atcgaaatcg acatgatcgc taggcgagcg ggcttcgcgc aggaccgctt
     5041 cgggcgtgag ccatcctcgt cgggcgagat catcgatgtt gatcaagagg tgattgccgg
     5101 caaacgacga cgtcgactga tagggtgaat ttccgtatcc cgttggtccc agcggcagta
     5161 tttgccacca gcgctgaccg gtggcggcca aaaagtcgac gaaggcgtgg gcttcggcac
     5221 ccagatcacc aatcccgtgg cggccgggga gcgacgtggg atgcagcaat acgccggcgg
     5281 cccgaggaag tctcatccta cttttctccc gatcatcgcg tgaagattcg tcatcgcggc
     5341 acgcccttct caggcgtgcc cttgatccaa gaaccggtcg atttcctggc gaaagcgcgt
     5401 ctgatcctcg acgtgaggca tgtgaccaac gcccggcaga acgaccaacc gcgcgttccg
     5461 caatttatcg gcgagctcgg cggccactcg atgcgagttc agtaggtcga gctctcccac
     5521 caatacgagg caagggactt cgatctggcc gagccggctc cgcgtgtcgt gcgtggcggc
     5581 ggcctgcgcc tggcgagcaa acgcctccgg atcttgcggc cacggattgc gctcggcgaa
     5641 ctgcaccagt ccctcgacct gcgccggctg ccgatagaac gccggagcca ccaaccaagg
     5701 cagcaccgcc cgcgtgaatg caccagcatc gaccatgcgc cgcacgagca cccacgactc
     5761 aatgaccgct tggcgccacg ggtcgccccc ggcgtgggtg gagaccagga ccaagctctg
     5821 gacaagctgg ggatgacgca acgccagctc ttgtgcgacc agacccccca acgactggcc
     5881 cacgacatga gcaggacccg ccccgatcgc cccgagccag ccggcgacgt catcggccat
     5941 atcggccgtc gtgtatgcgc gatcggagcg gtcgctctgc cccgcgtcgc gtgagtcgaa
     6001 cgcgagggcg cgaaaccttg tgccgaaatg gcgcaacgcc cggctgaatg cccgattgtc
     6061 cccgcctaaa ccactgagga agacg
//
LOCUS       DATAQL010000470         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2337982, whole genome shotgun sequence.
ACCESSION   DATAQL010000470 DATAQL010000000
VERSION     DATAQL010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337982"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="VHS97_08145"
     CDS             complement(<1..1654)
                     /locus_tag="VHS97_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX3448212.1"
                     /translation="MIELPIDATLPQILGALQGRSVVVVAPPGSGKTTRVPPAILKAG
                     LLSTENPTAIVLQPRRVAARAAAARIADEQGWTLGEQVGYQIRLERRFSRNTRLRFLT
                     EGILTRQILADPFLENVGAVILDEFHERNLNSDLALALLREVREQVRPDLILVVMSAT
                     LDAEPVARFLDDCPIIRAEGRSHAITVDYRPVARPASAETLVPLIEEQLEDPRARGHI
                     LVFLPGVAEIRRVEKRLATAASRMGTVVLPLHGSLPAEDQDRALRPTDRRKVILSTNV
                     AETSLTIDGVSTVIDCGLARTVRYDAQRGIDRWELGRISRAAAAQRAGRAGRTGPGRC
                     IRLWSERDQNARPEFEQPEVHRVDLSATVLALHAWGTVDTAQFAWFDRPPPDRLAAAE
                     RMLVMLGALTGAPARISELGKQMLSLPIHPRLARLLMAARECGRGREGATVAALLSER
                     DIRARDSSAAPGRSPTATTVATCRSDVADRLDMLALAETMRFSHSLRSRGIDPGAARQ
                     VALVRDDLIGRERHHERNVARPGDVDDDDVDVLKWLLLAYPDR"
     gene            complement(1662..>2807)
                     /locus_tag="VHS97_08150"
     CDS             complement(1662..>2807)
                     /locus_tag="VHS97_08150"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX3448213.1"
                     /translation="FGQLLTGVDTLNQMAAVPVTVNGQASQPNNPVTITSASLSSTNP
                     NGVLLIDTTQATLNQTSIITVTATDPAKGSKTTQSFVVSVSAYGGPTSGTDLTLNLRP
                     YANDSAPTVAKNTATQVQLHGVNGNPAGSTSTLSYSLLTQPTHGTVSNFNPSTGTLTY
                     TPDQGFVGTDTLTYQTSATAPSGRTPATSVSNPGRVTFAVGGENTGTVNVVGNVLTVT
                     PVARRDLGTNTIDVSQVPNSSSPTGLGYVVTVDGIPDTTAVSTAGINKISVFGGKRAR
                     NKIVIDPSVTVPSVIDGGQGYRNKLTGGSVETREHGWFGHSTLVGGSGPNQLIGRAGL
                     VRFKPTKSTNLIFAGVPKKRTSHLNATPPGGTFFKLVRGRLVPVFKF"
BASE COUNT          502 a          843 c          941 g          521 t
ORIGIN      
        1 cgcgatcggg atacgccaga agcaaccatt tgagtacgtc gacgtcgtcg tcatccacgt
       61 cgcccggtcg tgccacattg cgctcatgat ggcgttcgcg cccgatcagg tcgtcgcgca
      121 ccaaggcaac ctgacgggcg gcgccaggat cgattccgcg cgagcgcaac gaatgagaaa
      181 atcgcatcgt ttcggccaag gccagcatgt cgaggcggtc ggcgacatcg gaccggcaag
      241 tcgctacggt tgtcgcggtc ggcgacctac ctggagccgc cgacgaatcg cgagcgcgga
      301 tgtcccgttc cgagagcaag gcggcgactg ttgcaccttc gcggccacgg ccgcattctc
      361 tggccgccat cagcaggcgg gccaggcgag ggtgaatcgg aagggaaagc atctgcttcc
      421 ccaattcaga gattcgcgcg ggggctccag tgagcgcacc cagcatcacg agcatgcgtt
      481 cggcggctgc cagtcggtcc ggaggcggac ggtcaaacca agcgaactgg gcggtatcga
      541 ccgtgcccca ggcgtgcaaa gccagcactg tcgcgcttaa gtcaacgcgg tggacttcgg
      601 gctgctcgaa ctccggacgg gcgttctggt cgcgctccga ccagaggcgg atacaacgac
      661 cgggccccgt acgtcccgcg cgaccggctc gttgggccgc cgcggctcga cttatacggc
      721 caagctccca gcggtcgatg ccccgctggg cgtcgtagcg gacagttcgg gccagtccgc
      781 aatcgattac ggtcgagaca ccgtcgatgg taagcgacgt ctcggcgacg ttggtgctca
      841 agatgacctt gcggcggtca gtcggtctga gcgcccggtc ctgatcctcg gcgggaagtg
      901 acccgtgcag aggcaatacc accgtgccca tgcgactggc cgcggtcgca agccgcttct
      961 cgacccggcg aatctcggcc acaccaggca agaacacgag gatgtggccg cgggcgcgcg
     1021 gatcctcgag ctgctcttcg ataagaggga ccagcgtttc ggcgctggcc ggccgggcga
     1081 ccggccgata gtccacggtt atggcgtgag atcgtccttc ggcacggatt atcgggcaat
     1141 catcgagaaa ccgcgcgacc ggctcggcgt caagcgtggc ggacatgacg accagaatca
     1201 ggtcgggacg gacctgctcg cggacctcgc gcaagagcgc caatgccaag tcgctgttca
     1261 ggttgcgctc gtgaaattcg tcgagaatga cggcgccgac gttttcgaga aaggggtcgg
     1321 ccaggatctg ccgcgtgagg attccctcgg tcaggaaccg aagccgggtg ttccgggaaa
     1381 accgccgttc gagtcggatc tggtatccga cctgttcacc caacgtccac ccctgttcgt
     1441 cggcgattct cgccgccgcg gcgcgggcag cgacgcgccg gggctgtagc acaatcgcgg
     1501 tcgggttctc ggtcgagagc agaccggcct tgaggatggc tggcggcacc cgagtggttt
     1561 tgcccgagcc gggaggtgcc accacgacca cggagcgtcc ctggagagcc cccaggatct
     1621 gaggaagtgt cgcatcaatg ggaagctcga tcatgcgaag ctcagaactt gaagaccggc
     1681 accagtcgac cgcggaccag cttgaagaaa gtaccgcccg gcggcgtggc attgagatgg
     1741 cttgtccgct ttttcggcac tccggcgaaa atcaggtttg tggatttggt cggcttgaac
     1801 cggacgaggc cggcccggcc gatcaactga ttcggcccgg acccgcccac gagggtcgag
     1861 tggccgaacc agccatgctc gcgcgtctcc acgcttccgc cggtcaactt attcctgtag
     1921 ccctgtccgc catcgatgac cgacggcact gtcacactcg ggtcgataac gatcttgttc
     1981 ctggcccttt tgccgccgaa gacactaatt ttattaattc ccgcggtgga aactgccgtt
     2041 gtgtcgggga taccgtcgac agtcaccacg tagccgagac ccgtggggga gctcgagtta
     2101 ggcacctgcg atacgtcgat cgtgttcgtg ccaagatctc ggcgggccac cggggttacg
     2161 gtcagcacgt tgccaaccac gttcacggtc cccgtgtttt cccctccgac cgcgaaggta
     2221 accctaccag ggttgctgac gctggtcgcg ggggttctcc ctgagggggc cgtcgcgctg
     2281 gtctggtaag taagagtgtc ggtgccgacg aaaccctggt cgggtgtgta tgtaagcgtc
     2341 cccgtggacg gattgaagtt gctgaccgtt ccgtgcgtgg gttgagtcag tagtgagtaa
     2401 ctcagggtcg aggtggaacc ggctggatta ccgttcaccc cgtgtaactg gacctgtgtc
     2461 gccgtgttct tggcgacagt aggggcagag tcattcgcgt agggtctgag gttgagagtc
     2521 aggtctgtgc ctgatgtggg gccgccgtaa gcggagactg ataccacgaa cgactgagtc
     2581 gtcttgctcc ccttcgccgg gtcggtcgcg gtgactgtga taatggacgt ctggttcagg
     2641 gtagcctggg tggtgtcgat gaggagaacg ccgttcgggt tggtcgaaga cagcgatgcc
     2701 gacgtgatcg tgaccgggtt gttcggctga gacgcttggc cgttgacggt caccggcact
     2761 gcagccatct ggttgagtgt atcaacgccg gtgagcaact ggccgaa
//
LOCUS       DATAQL010000471         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2031841, whole genome shotgun sequence.
ACCESSION   DATAQL010000471 DATAQL010000000
VERSION     DATAQL010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031841"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1384)
                     /locus_tag="VHS97_08155"
     CDS             complement(323..1384)
                     /locus_tag="VHS97_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448214.1"
                     /translation="MSILRYVLIAFTIVVLYYSMEEWVTGGRTSRSILVAAGNVGSVH
                     PADWPSAGNLLLALIWKCVGLILLLGAAGLAMVSRRSSSGRRALVAAGTGLISLVVAL
                     TVCQFVVVKPTFFGLLALLLLVILAVVIPACALFEKSLFGDFADLALLAYFVAEMALT
                     SVLWRLNAGGSFNYAIEAVVIACALTGRALARAFDGATSRRSFFPAALAALAVPVFAF
                     TDVKERLGKRQVERAGLARVSTLLRRPAAEIFFVDLPGANRLHGRLDMVYDPWLYPVF
                     ESIGLAEPRSIWLAEALSTGPVRVVVSTTSRPRLEGLGRTLHELGYEDAIRVGDYFIW
                     KKRARGGEQARAPVDDIVK"
BASE COUNT          388 a          480 c          421 g          234 t
ORIGIN      
        1 actcgggctc ggtgagaatc tgcagccgct cttcttcact aggggtaatc tgggccgaag
       61 cgggtgcgcc gaaacacgcg aaaagccccg ccaggaacaa ggcaatctga actcgaaatg
      121 tgatcgcctt catggactgc cgccttcaaa gccagccgcc agcgacgtcc ggctgaaacc
      181 taatcccggc cgcttcgatt tcgacaccga gggaattaat ctaataggtc ccacgagtgt
      241 aacacctgtg gacccgcacc ggaagggtcg cgaaatcgca atcgcgagtc gtgaaatctg
      301 ttcgacctct tcagaccgga attcatttca caatgtcatc gaccggcgcc cttgcctgct
      361 ctccgccacg agcgcgcttt ttccagataa aatagtcccc gactctgatc gcatcctcat
      421 aaccgagctc gtggagcgtg cggcccagac cctcgagtct cgggcggctc gtggtcgaaa
      481 cgacaacgcg caccgggccg gtggaaaggg cctccgcgag ccagatcgat cgtggctcgg
      541 ccaggccgat cgactcaaaa acggggtaaa gccacggatc ataaaccatg tcgagccggc
      601 cgtggagacg gttcgcaccc ggaagatcga cgaagaagat ctcggccgca ggacggcgca
      661 agagcgttga cacccgagct aacccggccc gctctacctg gcgcttgcca agacgttcct
      721 tcacatctgt gaatgcgaaa accggaacgg ccagcgctgc cagggctgct ggaaaaaaag
      781 accgccgcga cgtggcaccg tcaaaggcac gcgcaagcgc tcgcccggtc aaggcgcacg
      841 cgatgaccac cgcctcgatc gcgtaattga acgagcctcc tgcgttcaac cgccagagaa
      901 ctgatgtgag tgccatttcg gcgacgaagt aagccaagag agccaaatcg gcgaaatcac
      961 caaacagcga tttttcaaac aatgcgcagg ccggaatcac cacggccaag ataacgagca
     1021 aaagaagcgc caatagcccg aagaaagtcg gcttcacaac cacgaactgg cagaccgtca
     1081 gggcgaccac caaactgatg agacctgtgc ccgccgccac gagcgcccgc cgtccgctgc
     1141 tcgaccttct cgataccata gccagaccgg cagcccccag caaaaggatc aaacccacgc
     1201 atttccagat cagagccagc aagagattgc cggcggaggg ccaatccgca ggatggaccg
     1261 atccgacatt gcctgctgcg accaggatcg agcgagacgt cctgcctccg gtaacccatt
     1321 cctccatgct gtagtagagc acgacgattg tgaatgcgat caatacatat cgcaaaatcg
     1381 acacgaggcc gagccggcac cgagctcggt tctacgcaag caaaaagaga ctgatcagag
     1441 gggccatgac gaaatgctgc ttgatacaga gggccacgcc gaagcaggca aatgcagccg
     1501 ccagtttgcg aagagcggcc cgt
//
LOCUS       DATAQL010000472         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5391258, whole genome shotgun sequence.
ACCESSION   DATAQL010000472 DATAQL010000000
VERSION     DATAQL010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5391258"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VHS97_08160"
     CDS             <1..252
                     /locus_tag="VHS97_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448215.1"
                     /translation="PQAWSAASVFLLLQASLGLEIDAIAGRVVFSRPALPLALPELRI
                     FDLNVGNGRVDLLLTRHDEDVSVRVSRRQGQHVHVSIDQ"
     gene            757..1827
                     /locus_tag="VHS97_08165"
     CDS             757..1827
                     /locus_tag="VHS97_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3448216.1"
                     /translation="MPALKPLALGVLVWAAMDNVVQADAITWPSNNAWVTYFAGQGTN
                     GDGNPAPTNSPVALTQPPQPAAVTVQPSPQPPTAAPAPTPAPAPVAAQTVVADAPAMQ
                     APVASYTPTLSAMASTLSSGPVDAYVNLGSGPFPLQSTITTGNAQPWYSSSQISSLFG
                     GQPTSQQIQSFDNTILQRVQQTFSQSGVNVTLTSDPSVAAQHTLSLVSNTSAASLTNA
                     IGMTQVGSSGFSFIDHIASSAQSVDQLEWIVAHNISHELMLAFGVPENYDKTGNFVDS
                     RVADWAMMVSPTSTFSPAAAQALDQALSAQNGGNSGQLGAQLVGSESPVPEPTTIALW
                     AAAGAAIVIAKRRRSHCGRAAV"
BASE COUNT          411 a          684 c          534 g          409 t
ORIGIN      
        1 ccacaggcct ggtcggccgc ttccgtcttt ctcctcctgc aggcgagttt ggggctcgag
       61 atcgatgcaa tcgccggacg agtggttttc agccgccctg ctctgccgtt ggcgctaccc
      121 gagctcagaa tcttcgacct caacgtcgga aacggccgcg tagatcttct tctgacaaga
      181 cacgatgaag acgtctcggt ccgcgtctcg cgtcgccaag gacagcacgt gcacgtctcg
      241 atcgaccagt gataccccgg ctcaaaagga tagccaggtt ggctgcgctc ggatttcctt
      301 aaactcccta ctccctacta accaatgact aatgaccaat cttcgtcgtt gttgcaaatg
      361 cacctcactt ttgtaagaat cggcacgatc cgcacgaccg ggcattcgtc gctgttcacc
      421 gtcgagtacg cggccggtcg ccaagaaatc ttaacataaa cttgatccca actggacttt
      481 ccgttcattt cacgccgtcg caaccgctcg attgaagatg aatatcgatc atcaaccccg
      541 tcttggcata aggtttgctt tcagacggtt cgctttccca caaacatctc ccctgcccac
      601 ggcggtttcg gactttccaa aaaagcgggg cccatacgcc aaggcggccg gctttccagc
      661 cctgcctcgg agttggggtg cggattgagc tgcagtgcgg tcgcggttgg gatcatcagg
      721 atggtgatct ccgtcggctc gaacaggagg ttcgcgatgc ccgctttgaa acccctggcc
      781 ctgggcgtgc tcgtatgggc ggcgatggac aacgttgtcc aggccgatgc aatcacctgg
      841 ccgagtaaca atgcttgggt gacctacttc gctggccagg gtaccaatgg tgacggtaat
      901 cctgctccca ccaattcacc ggtcgctctg acccagccgc cacagcctgc ggcggtaacg
      961 gtccagcctt cgccccagcc gcctacagct gcacctgcac ccacaccagc accagccccg
     1021 gtcgcggctc aaacagtcgt agcagacgcg ccagccatgc aagccccagt tgcgagctac
     1081 accccgacac tctcggcgat ggcgtcgact ctctcttccg gtcccgtcga tgcatatgtc
     1141 aacctgggca gcggcccatt ccccctgcaa agcacaatca cgaccggcaa cgctcagcca
     1201 tggtacagca gctcgcagat ctccagcttg ttcggcggcc aacccacctc tcagcagatc
     1261 cagagtttcg acaacaccat cctccaacgc gttcagcaga ccttcagcca aagcggcgtc
     1321 aatgtaacgc tgacctccga cccgagcgtc gccgcccagc acactttgag cttggtctcg
     1381 aatacgtcgg ctgcatcgct gaccaatgcg atcggcatga cccaggtcgg aagcagcggt
     1441 ttcagcttca tcgaccatat cgcatcctcg gcccagtcgg tcgaccagct cgaatggatc
     1501 gtggcgcaca acatctccca cgagctgatg ctcgctttcg gagttccgga aaactacgac
     1561 aagaccggca atttcgtcga ctcgagagtg gccgattggg cgatgatggt tagcccgacc
     1621 tcgacattta gccccgccgc tgcgcaggcg ctcgaccagg cgttgtccgc acagaacgga
     1681 ggaaattcgg gccaactggg tgcgcaatta gtcggctcgg agtctcccgt ccctgagccg
     1741 acgaccatcg ccctctgggc cgcggcgggc gcggcaatcg tgattgcgaa acgccgcaga
     1801 tcgcactgcg gccgcgccgc cgtgtgaacg atcgtcattc gcctcacaca ttgatcggaa
     1861 gattcgctcg ctctcgcctt tgcatacgat caacgccaac tgtgtcggtt ctgcttaaca
     1921 tcgatcggcg attgtctcgc ccctcacctc gaggccagcc acttggagca gcttaagaag
     1981 tcactccatg acgcggccga caagatcaag caagcacaac ggaccgcccg tcccgctt
//
LOCUS       DATAQL010000473         4214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3864790, whole genome shotgun sequence.
ACCESSION   DATAQL010000473 DATAQL010000000
VERSION     DATAQL010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3864790"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VHS97_08170"
     CDS             complement(<1..886)
                     /locus_tag="VHS97_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3448217.1"
                     /translation="MIPLARDNPRIVGPLPGPKSMAWLERDDRVMSPSYTRVYPLVVR
                     RGRGTMIEDMDGNRFLDFTAGIAVTNVGHSHPQVVAAIRRQANRLIHMSGTDFYYAPQ
                     VRLAERLAALAPGPEPKRVFFTNSGAEAIEAALKLARRHTGRNRVVAFVNAFHGRTYG
                     AMSLSGSKPMQRRGFSPLVPDIHHTPYGDVESVKALFRTICPPDELAEIFVEPIQGEG
                     GYIVPPPDFLPGLRRLCDEHGVLLVFDEVQSGMGRTGKMFAAEHWGVAGDIMCLAKGI
                     ANGLPLGAIVAKASVMDWP"
     gene            1069..2160
                     /locus_tag="VHS97_08175"
     CDS             1069..2160
                     /locus_tag="VHS97_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448218.1"
                     /translation="MIKTVVVGHGLAGRAFHCPLIRRQRGLYLHGIVARDPEIRSQAV
                     SLWGPGVHGYADLDEALTDPEVQLVVIATPHDSHADLAIRALENGRHCVVDKVMALTT
                     ADADQMIAARDRSGCMLSVFHNRRWDWDYLTVREALNQGIFGRPLLIESSVCRHAPPR
                     SWRGHVQSAGTILHDWGAHLVDQALQLGLGPCRRLTAWLTPAPWANVDSGAHGRIVLE
                     FDDTLFQIESSRICRLDRPRWWIIGSEAGFVKFGIDPQEDALRAGDIDHAAEPPEFEA
                     IVRRADGAGQTLETRFPTVRGHWDAYYRNIALHLESDSALAVTAEHAREVVRLLEAAT
                     RSAREHMTVTGPWGQNSNVTIVANQAADG"
     gene            2310..3047
                     /locus_tag="VHS97_08180"
     CDS             2310..3047
                     /locus_tag="VHS97_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448219.1"
                     /translation="MIDMKCPSCGAGGRIPNEKVNTRLICKKCLRVFHVTPAGNTVLG
                     EPAAPKEESKPRSTRESIRQDPVQGFDELTSRLSSIRLPQVQPMTLGLIAGAVLLSAF
                     VYWFFFYKQSLATRTESIARAIVKSDMKTVVDLAAPGTEMDTMRWYHDVYKSYLDLKL
                     ALAGQDAGISIQLPGGESGGTTRVNLVFSKDGLRFDGTIFSDALQPNPSLSSAKQSLD
                     VPLFWVKDLWGNWLLDGTKTFAGTASP"
     gene            3326..4207
                     /gene="murB"
                     /locus_tag="VHS97_08185"
     CDS             3326..4207
                     /gene="murB"
                     /locus_tag="VHS97_08185"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX3448220.1"
                     /translation="MHLFDDLRGTVKLDEPLAPLVWFRLGGPAAYFASPRQLDELIAL
                     LKRCRQEGVPFKILGGGSNVLVRDEGIAALVIHLESPFFSDVTVRDNVVQAGAAVPLT
                     ALISHSARAGLAGLEVLTGIPGTVGGALRCNAGGRQGAIGQFVRRATVLDPADIVQVR
                     ERDDLSFADRDSNLDEPLILSAEFELASEDPEAVVRRMRRIWIIKKENQPYGHQSSGS
                     IFRNPSPDVSAAALIDQAGLKGTRCGGAEVSDRHANFIVAHPGAKASDVLELIDRIRQ
                     RVWQQFGYELELQIQVW"
BASE COUNT          805 a         1323 c         1275 g          811 t
ORIGIN      
        1 tgggccaatc catgaccgag gccttggcca cgatcgctcc caagggcagc ccgttggcga
       61 tgcctttggc caggcacatg atatcgccag ccacccccca atgctctgcg gcaaacatct
      121 ttcccgttcg gcccatgccg ctttgaactt cgtcaaagac caagagcacg ccatgctcgt
      181 cgcaaagccg acgcagtccc ggcagaaaat ccggcggggg tacgatgtag cccccttcgc
      241 cctggatcgg ctcgacaaaa atctccgcca gctcgtccgg ggggcagatc gtccggaaga
      301 gtgctttcac gctttcgacg tcgccgtaag gggtatgatg gatgtcgggc acgagcggag
      361 aaaagccccg gcgctgcatc ggcttggacc ccgagagcga catggctccg taggtccgcc
      421 cgtgaaacgc attgacgaac gcgaccaccc ggttgcgccc cgtatgccgc cgggccagct
      481 tgagggctgc ctcgatcgcc tcggcaccac tgtttgtgaa aaagactcgc ttgggttccg
      541 gaccaggggc taaagcggcc aatcgctccg ccagccggac ctgcggcgcg tagtaaaaat
      601 cggtccctga catatgaatc aaacggttcg cctgccggcg tatcgccgcg accacttggg
      661 gatgcgagtg cccgacattg gtcaccgcga tcccggccgt gaagtcgaga aatcggttgc
      721 cgtccatgtc ttcgatcatc gtcccccggc cgcggcgcac aacgagcggg tacactcgcg
      781 tgtacgaggg agacatcact cgatcatcac gctccagcca ggccatgctt ttgggcccag
      841 gaagcggtcc gacgatccgc ggattgtcgc gtgcgagagg aatcattcct ttatccttcc
      901 tggtgctcgg tgttcgatcg actggcgcgg ccaactacta tgattatcag ggaatttcgc
      961 cttttggtca aatcattccg aacgcgcgcg ttctgatcta tgatgggtca tggtgacacc
     1021 ggccaacgct cggtcgtggc gaagaaccct ccgaatcggg atgcggacat gataaaaacg
     1081 gtcgtggtcg ggcacggctt ggcgggccga gcctttcact gtccattgat acggcgccag
     1141 cgcggactct acctgcacgg gatcgtggcg cgcgaccccg agatccgttc gcaagcggta
     1201 tcgctttggg gccctggcgt gcacggctac gcggatctgg acgaagccct gaccgacccc
     1261 gaagtgcagt tagttgtgat cgccacaccg cacgacagtc acgccgatct ggccatccgt
     1321 gcccttgaga acgggcggca ctgcgtggtc gacaaggtga tggctctcac cacggccgac
     1381 gccgaccaga tgatcgcggc ccgcgaccgg tccggatgca tgctttcggt ctttcacaac
     1441 cgcaggtggg attgggacta cttgaccgtc agggaagccc tcaaccaggg cattttcggg
     1501 cggccgctct tgatcgagag ctcggtctgc cgccacgcgc caccacgaag ctggcgcggt
     1561 catgttcaat ccgccggaac catcttacac gactggggcg cgcatctcgt cgaccaggcg
     1621 ctccagctcg gtctgggccc gtgccgccgc ttgaccgcct ggctaacgcc cgctccctgg
     1681 gccaacgtcg acagcggcgc tcatggtagg attgtcctcg agttcgacga cactttgttc
     1741 cagatcgagt cgagccgcat ttgccggctg gaccgccccc gctggtggat catcggctcg
     1801 gaggcggggt ttgtgaaatt tggcatcgat ccccaggaag atgcgctacg cgccggcgac
     1861 atcgaccatg ctgccgagcc acccgaattc gaggctatag tccgccgggc cgatggcgcg
     1921 gggcaaaccc tcgaaactcg gtttccgacc gtccgcggcc actgggacgc gtactatcgc
     1981 aatatcgctc tacaccttga aagcgactct gcgctggcgg tgaccgccga acatgcccgc
     2041 gaggtcgtcc gcctgctcga ggccgcgacc cgttccgcac gcgagcatat gactgtcacg
     2101 ggaccttggg gtcaaaattc gaacgtcacc atcgttgcca accaagcggc ggacggttag
     2161 cttgagcatg ggtagacttc ccaaagccgc atccctactc gacgaacgaa ttgcggagat
     2221 gcgaagtgcg gcgcgacggt caacctggaa acctccgagg gattcgatga cgagacattc
     2281 gatacaacgg tcacggaggt acactactaa tgattgacat gaaatgtccg agctgcgggg
     2341 ctggcggacg gatacccaac gaaaaggtaa atacccgcct tatatgcaag aaatgcctga
     2401 gggtctttca cgtcacgccg gctggcaaca ccgtgcttgg cgaaccggct gcccccaagg
     2461 aagagtccaa gcctcgttcg acccgcgagt cgatccggca ggatccggtc cagggtttcg
     2521 acgagctgac gtcacgactg tccagtattc ggcttcctca ggtccagccg atgaccctcg
     2581 gtttgatcgc gggcgccgtc ttgctctcgg cgttcgtcta ttggtttttc ttctacaagc
     2641 agtcgctggc gacccgaacc gaaagcatcg cgagagccat cgtcaagagc gacatgaaaa
     2701 ccgtcgtcga tcttgccgcc ccagggacgg aaatggatac gatgagatgg tatcatgatg
     2761 tttacaagtc ttacctggac ctcaagctgg ctctagccgg gcaggacgcc gggatctcga
     2821 tccaactccc tggcggagaa agcgggggca caacacgggt caatctggtc ttctcgaagg
     2881 acggattgcg attcgatgga acgattttca gcgacgccct gcagcccaac cccagcctct
     2941 ctagtgccaa gcaatcgctg gatgtcccgc tcttctgggt gaaagaccta tggggcaact
     3001 ggctgctcga cggaaccaag accttcgctg gtacggcgtc gccctgaagc ggcggaatcg
     3061 ccagggcgcc accggacctc tcattgaacg aggtactact cgccggggct ggcacgggcg
     3121 atctcacggc ctcgcggaca cggctgcatg gttgcgagac gtcgtcccga cgtcgcttga
     3181 cgaccgaggc acttgaggct acgatctttt ggactatgga tttacgtcga cgaactcgac
     3241 gcaatccatc cgtcgaatcg attggacctc tcagcgggcc tgcgcgggcg gtgcgctgct
     3301 tgatcgaggt ggggaatccc aaccgatgca cttatttgac gacttgcgtg ggaccgtgaa
     3361 actcgacgag ccgctggctc ctctggtttg gttccgtctg ggaggacctg ccgcgtactt
     3421 tgccagtcct cgtcagctcg acgagctgat cgctctcttg aagcgatgcc ggcaggaagg
     3481 ggtcccgttc aagattctgg ggggaggatc gaacgtcctg gttcgcgacg aagggatcgc
     3541 cgcgctcgtg attcatctcg aaagtccctt tttctccgac gtgacggttc gggacaatgt
     3601 ggtgcaggcc ggggccgcgg tcccactcac agccttgatc tcgcattccg cccgggccgg
     3661 actggccggc ctcgaggttc tgacgggcat accgggcaca gtcggcggtg ccctgcggtg
     3721 caacgccggc ggccgacagg gggcaatcgg ccagtttgtc cgccgcgcga ccgttctcga
     3781 cccggctgat atcgtccagg tgcgggaacg agacgacctc agcttcgccg atcgcgactc
     3841 caacctcgac gagcctttga tcctctccgc cgagttcgag ctcgccagtg aggatccgga
     3901 ggccgtcgtt cgcagaatga ggcgcatctg gataatcaag aaagagaatc aaccgtacgg
     3961 ccatcagtcg tccgggtcaa ttttccggaa tccctcgccc gacgtgtcgg cggcagccct
     4021 gatcgatcag gctggactca aaggcacccg ttgcggcggc gccgaagtct ccgatcgcca
     4081 tgccaacttc atcgtcgctc atcccggcgc caaagcctcc gacgtgctcg agttgatcga
     4141 ccggatccgc cagcgcgtct ggcagcagtt cggttacgag ttggaacttc aaatccaagt
     4201 ctggtaagct tttg
//
LOCUS       DATAQL010000474         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5085781, whole genome shotgun sequence.
ACCESSION   DATAQL010000474 DATAQL010000000
VERSION     DATAQL010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5085781"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1301
                     /locus_tag="VHS97_08190"
     CDS             312..1301
                     /locus_tag="VHS97_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448221.1"
                     /translation="MRWVGIDEAGYGPNLGPLVMTAVVAESTTVQIGDPADVRALDFW
                     GALAKTVDRAGGDPDRLWVDDSKAIYQGGTGRDRLEIACMAAVHATGQALPGCLRTLI
                     EILGAGTLADVELTHWIDGDEEHLPWPLSVPRDALDALLARTPLYAGKKAWRITAVAC
                     VVIGPARFNNGLEAHGSKAAVHFSAFDVLLKQVWNRAADGRRTFVQGDKHGGRHYYLP
                     PLSQAFPEAWIDRGPEGPDLSRYTIRDGDRRLELSLAPRADQTDGLVALASIVSKTVR
                     ELWMDVFNAYWRRRIPELRPTAGYPNDSLRFRREIENLAVAQGHEPARWWRIK"
     gene            complement(1350..1568)
                     /gene="infA"
                     /locus_tag="VHS97_08195"
     CDS             complement(1350..1568)
                     /gene="infA"
                     /locus_tag="VHS97_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX3448222.1"
                     /translation="MPKEEPIRTEGRIVEALPNTQFMVELENGHRILAHIAGKMRKNF
                     IRIVPGDRVTVEITPYDLSKGRIVYRER"
     gene            complement(1843..>2062)
                     /locus_tag="VHS97_08200"
     CDS             complement(1843..>2062)
                     /locus_tag="VHS97_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3448223.1"
                     /translation="KVDRAGRVYVAVALGIWVFEPDGRLLGILSMPVRPSNLAWCDSD
                     ARGLAITAGDAVFHVRLRVEGIMPPFMP"
BASE COUNT          400 a          612 c          662 g          388 t
ORIGIN      
        1 gttgagtttg gccgtttgcc cagagccggc gatggcaatg ccgggctggt gaaggtgagc
       61 gataagcgac gcagccgtcc ctcggtgtgc ttggaaaccg cctgaactca agagcatttt
      121 cggctcgagt gattcgagcg acagcatttg gccccttgtc gaggttcggc gtggcattga
      181 aatcggcctc cttaaatgct cagatcaagg gcgcgacgcc ttgaaaggga attgcgatcg
      241 cggcgatagc aataggcgtt gctattctca actttaggtc gcgacggacg aacacggaag
      301 gaaatgcgac tttgcgctgg gttggaatcg acgaagccgg ctatggaccc aacttggggc
      361 cgctagtgat gacggcagtc gtcgccgaat cgaccaccgt ccagatcggc gatccggccg
      421 acgttcgcgc gctcgatttc tggggggctc tggcgaagac cgtggatcgg gccggcggcg
      481 accccgatcg gctgtgggtt gacgattcaa aggcaatcta tcagggcggg acggggcgcg
      541 atcggctcga aattgcctgt atggcagcgg tgcacgcaac aggacaagcc ctacccggct
      601 gccttcggac gctgatcgaa atcttgggcg ctggcacgct cgcggatgtc gagctgaccc
      661 actggatcga cggggatgag gagcatttgc cctggccgct atctgtgccg cgtgatgcgc
      721 tcgatgcgct gctggcgcgc acgccgcttt atgctggcaa aaaggcctgg cggatcaccg
      781 ccgtggcgtg cgtcgtcatc ggaccggctc ggttcaacaa cggtctggag gcgcatggat
      841 caaaagccgc ggttcatttc tcggcctttg acgtgctctt gaagcaagtc tggaaccgag
      901 ctgccgacgg gcgccgcacg ttcgttcaag gtgacaagca cggcggacgc cattactacc
      961 ttccgccgct ttcacaggcg tttcccgaag cctggatcga ccgcggtccc gaaggcccgg
     1021 acctgagccg ttacacgatc agggacggcg accgtagact tgagctaagc ctggcccctc
     1081 gtgccgacca gaccgatggt ctggtcgcgc tggcgtcgat cgtcagcaag acggttcggg
     1141 aactctggat ggacgtcttc aacgcctact ggcgacggcg aatcccagag cttcgaccga
     1201 cggccggtta tccgaatgac tcccttcggt tccgcaggga gatcgagaac ctcgcggtgg
     1261 cccaagggca tgagccggcc cgctggtggc gcatcaagta gggctgttga tcaagccaaa
     1321 gttccggcca ggcggtcggg cgagttggct cagcgttcgc ggtagacgat acgacccttg
     1381 gagagatcgt acggtgtgat ttcgacggtc acgcggtcac cgggcacaat gcggatgaaa
     1441 ttcttgcgca ttttgccggc gatgtgggcc agaatgcggt gtccgttttc caactcgacc
     1501 atgaactggg tattgggtag ggcctcgacg atgcgcccct cggtacgaat cggttcctct
     1561 ttgggcacgc ggatctcctg aagtcgacca gcgccggccg cgtcgttggt ctgttcgtgt
     1621 ctatacaaaa ttgccacgcc gaccggtctc agcacgctgc gctacgcgcg aacccaggcg
     1681 ggtcgactgc cgctggagcc cgccggaggg ccggatcgaa acgtgtgaga ttcgcctgaa
     1741 tcttgagaat aatgcattac gatgaacttg aacagtacac ccggcgaaag aaatcagcga
     1801 ttacgggtca gcgtctggca aacctgaccc gacaatcgcg gttcacggca tgaacggcgg
     1861 catgatgcct tcgacccgca ggcgcacgtg aaacaccgcg tcgcccgcgg tgatcgccag
     1921 acctcgcgca tcggagtcgc accaggccag gtttgacggc cgaaccggca tcgacaaaat
     1981 tcccagcagg cgtccgtccg gctcgaacac ccagatcccc agcgcaacgg cgacgtaaac
     2041 ccggccggcc cggtcaacct tc
//
LOCUS       DATAQL010000475         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5085847, whole genome shotgun sequence.
ACCESSION   DATAQL010000475 DATAQL010000000
VERSION     DATAQL010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5085847"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VHS97_08205"
     CDS             <1..404
                     /locus_tag="VHS97_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448224.1"
                     /translation="KNNYLEPIERTGGKSAGYVDKWIVYGTVDGKQLFSACELTVEPG
                     RSITIKDPGSYGLIVVQGSGLIGKLAVDSPNFIRFGELTHDEVFVAAKRAAEGVTFHN
                     TGSEPFVSLRYFGPDTHGDLPKVGDHNKSGK"
     gene            453..1502
                     /locus_tag="VHS97_08210"
     CDS             453..1502
                     /locus_tag="VHS97_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3448225.1"
                     /translation="MSGSHSNTFPKIHNAMWPGIVGKGSPGAEPTIDLDTMLNLTAAA
                     SVDGIKFEGVDIFLYDPHINIDIGDDGLKAVAAKIADKGFVVGTVVAPVWFDASAMGD
                     DRQRDNYLTAIRKACRIAKKLRELGVRPNGVVRIDSAATPGDWAQDPAGNQKNIAETL
                     KQAAVIARDHGERLAAEGEICWGGMHSWRTMVDLLEQVGEPETVGFQADMAHTLLYTL
                     GENAPEDRILPKDYDWKDPATLDAALKTLTSALRPWTIDFHVAQNDATVFGSGTHDHT
                     GRHCLAADPNGKLNIPHHAGFWLRDENGKLTKTIRHICWDGCMFPNAVMTDPKTWNDI
                     LAAMIAVRDAHGWKE"
     gene            1522..2670
                     /locus_tag="VHS97_08215"
     CDS             1522..2670
                     /locus_tag="VHS97_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448226.1"
                     /translation="MSTKKPLNIGLVGYGFMGRTHSNAYKRVNDFFDVPYRPVLKAVC
                     GRTTEGAKAFAEQWGYESTETDWRKLIERKDIDAIDICTPNNTHAEIALAAAATGKMV
                     LCEKPLSMNLVEGQKMVDAIEKAGVPNTVWYNYRRVPAVTLAKNLIDAGRLGKIFHYR
                     ANFLQDWTISADLPQGGTGLWRLDVAAAGSGVTGDLLAHCIDTALWLNGSINSVSAMT
                     ETFIKERKHNLTGKVEPVGIDDACAFLCRFDNGSLGLFESTRYARGHKAFYTLEINGE
                     KASIKWDLEDLHRLEYFDHRDDSIVRGWHSVHVTDGDMPYMKQWWVPGLQIGYEHSFV
                     HQFADFLENVAKGQPTAPTFRDALATQAVCDAVLDSAENSKWESVVPV"
     gene            2682..>3089
                     /locus_tag="VHS97_08220"
     CDS             2682..>3089
                     /locus_tag="VHS97_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448227.1"
                     /translation="MDIETRPRSGESHPSIGVAMLLIAGATVGIWLALGELRSRNVGG
                     GPDDHLAKVVFVFVFALGGFALVGVPLLLWTARRRPWGAGRLLWLSHGTAAWLLWPPI
                     VYQRVEGRTAGSMSAICYFYGTPLMALYVTLALL"
BASE COUNT          636 a          943 c          942 g          568 t
ORIGIN      
        1 agaagaacaa ttacctcgag ccgatcgaac ggacgggcgg caagtcggcc gggtacgtcg
       61 acaagtggat cgtgtacggc acggtcgacg gcaagcagct cttcagcgcg tgcgagctca
      121 cggttgagcc tggccgctcg atcaccatca aggacccggg ctcctacggt cttatcgtgg
      181 tgcaaggtag cggcctgatc ggcaagcttg ccgtggacag cccgaacttc atccggttcg
      241 gcgagctcac tcatgacgag gtttttgtcg cggccaagcg cgctgccgaa ggggtcacgt
      301 tccacaacac ggggagcgag cccttcgtga gccttcgcta tttcgggccc gacacccacg
      361 gagatctgcc caaggtcggg gaccacaata aatcagggaa gtgaggtgcc gcgatgtcgg
      421 tgacttcata gcttcaaaca aagggattct ccatgagcgg cagccattcc aacacgtttc
      481 ccaaaatcca caacgcgatg tggcctggca tcgtcggcaa agggagcccc ggcgccgaac
      541 ctacgatcga cctcgacacc atgctaaacc tgacggccgc ggccagtgtc gacggcatca
      601 agtttgaagg ggtcgacatc tttctgtacg acccccacat caacatcgac atcggcgacg
      661 acgggttgaa ggccgtcgcc gccaagatcg ccgacaaggg gttcgtcgtc gggacggtgg
      721 tagcgcccgt ctggtttgac gcctcggcca tgggagacga ccgccagcgc gataactacc
      781 tgacggcgat ccgtaaggcc tgccggatcg ccaagaaact gcgcgagctg ggagtccggc
      841 ccaatggcgt ggtccggatc gactcggccg ccacgcccgg cgattgggct caagacccgg
      901 cgggcaacca gaagaacatt gccgaaacgc tcaagcaggc cgcggtgatc gcgcgcgacc
      961 acggggaacg actggccgcc gagggggaaa tctgctgggg cggcatgcat tcatggcgaa
     1021 ccatggtcga cttgctggaa caggtcggcg agcccgagac cgtcgggttt caggcggaca
     1081 tggctcacac gttgctttat acactcgggg aaaatgctcc cgaggaccgg atcttaccca
     1141 aggactatga ttggaaagac cctgccactc ttgacgccgc cctcaaaacc ctgacgtcgg
     1201 ccctgcgacc ctggaccatc gactttcacg tggcccaaaa cgacgccacc gtcttcggat
     1261 cgggcacgca cgaccacacc ggccgccact gcctggcggc cgatcccaac ggcaagctca
     1321 atattcctca tcacgccggc ttctggctac gcgacgagaa tggcaagctc accaagacca
     1381 tccgccacat ctgctgggac ggctgcatgt ttcctaacgc cgtcatgacc gacccgaaaa
     1441 cctggaacga catcctggcg gcgatgatcg cggtccgcga tgcccacggg tggaaagagt
     1501 aaggcaaact cggagttaaa aatgagcaca aaaaaaccac tcaatattgg cttggtcggc
     1561 tacggcttca tgggccggac tcattccaac gcctacaaac gtgtcaatga cttctttgac
     1621 gtaccctatc gacccgtctt gaaggccgtt tgcggcagga ccaccgaggg ggccaaggct
     1681 tttgccgagc agtggggcta tgagtcgacc gagaccgact ggcgcaagct gatcgagcgg
     1741 aaagatattg acgcgatcga tatatgcacg ccgaataata ctcatgccga gatcgccctg
     1801 gccgcggctg ccacgggcaa gatggtcctc tgcgagaaac ccctgtcgat gaatctggtc
     1861 gaaggccaga agatggtcga cgcgatcgag aaggccggcg taccgaacac agtctggtac
     1921 aactatcgtc gcgtgcccgc ggtgacgctc gccaagaatt tgatcgatgc cggacggctg
     1981 ggcaagatct tccattatcg cgccaacttc ctccaggact ggacaatctc ggccgacttg
     2041 ccgcaagggg gaaccggcct ctggcggctc gacgtggccg cggccggatc cggtgtcacc
     2101 ggcgacctgc tggcgcattg catcgacacg gcactctggt tgaacggctc gatcaattcg
     2161 gtgtccgcca tgaccgagac gttcatcaag gaacgaaagc acaatctcac gggcaaagtc
     2221 gagccggtgg gcattgacga tgcttgtgcg ttcctttgcc gtttcgacaa cggctcgctc
     2281 ggactctttg agtcgacccg ctatgcccga ggccacaagg cgttttacac cctcgaaatc
     2341 aatggcgaaa aggcgtcgat caagtgggat cttgaagacc tgcaccgcct cgagtatttc
     2401 gatcatcgcg acgattcgat tgtcaggggc tggcactcgg tgcacgtcac cgacggcgac
     2461 atgccctaca tgaagcaatg gtgggtgccg ggactccaga tcggctacga gcacagcttc
     2521 gtgcatcagt tcgccgactt cctcgaaaac gtggccaaag gtcagccgac agcgcccacg
     2581 tttcgcgatg ccctggcgac gcaggccgtc tgtgacgccg tgctcgattc ggccgaaaac
     2641 agcaagtggg agtctgtcgt gccggtctga ggaggctttc gttggacatc gagactcggc
     2701 ctcgcagcgg cgaatcgcac ccgagcatcg gcgtggcgat gctcctgatc gccggcgcga
     2761 cggtcgggat ctggctggct ctcggggagc tccggtccag aaacgtcggc ggaggtcccg
     2821 acgaccacct cgccaaggtc gtttttgtct tcgtctttgc gctcggcggc tttgccttgg
     2881 tgggcgtacc gctgctgttg tggacggctc gacgccggcc gtggggagca ggtcgactcc
     2941 tgtggttgtc acatggaacg gccgcctggc tgctctggcc tccgatcgtg taccaacgtg
     3001 tcgagggcag aactgcgggc tcgatgagtg cgatttgtta cttttatgga acgccgctca
     3061 tggcactcta cgtcacgctc gcgcttttg
//
LOCUS       DATAQL010000476         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5238958, whole genome shotgun sequence.
ACCESSION   DATAQL010000476 DATAQL010000000
VERSION     DATAQL010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5238958"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /gene="gatB"
                     /locus_tag="VHS97_08225"
     CDS             complement(<1..1311)
                     /gene="gatB"
                     /locus_tag="VHS97_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX3448228.1"
                     /translation="MDYEIIIGLEIHVQLQTQSKMFSPSGTDFGLPPNTQTDPVSLGL
                     PGTLPVMNRKAFDLALKTAVALHAEIAPFTKWDRKNYYYPDLPKNYQISQYDLPFSRG
                     GHLEIPLRKDGGGGGRCGLTRIHLEEDTGKLMHGDGGFSEVDLNRAGIPLLEIVTEPE
                     IRTPADARGCLEELRLTLRYLEVSDCEMQEGSLRCDANVNLHIMANGQKIATPIVEIK
                     NLNSFRAVEKALHYEVERQYAKWREDGKTINDAPKTTRGWNDVEEVTKPQREKETAAD
                     YRYFPEPDLVPVVVDAAWVERVRSSIGELPAARRHRFETDYGLSVYDANVLVEQGQDV
                     ADYYDAVAKATGEFKLASNWIQQDVLRTIKEKKLTVAEFPVRPDQLAGLINRVKAGEL
                     NTNQGRDILGQMIATGEAAETIIARGGYQMVSDRDALAVAVAAAI"
     gene            complement(1322..2812)
                     /gene="gatA"
                     /locus_tag="VHS97_08230"
     CDS             complement(1322..2812)
                     /gene="gatA"
                     /locus_tag="VHS97_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX3448229.1"
                     /translation="MSNENTTAISLMDRLSAGEITSESIVRDLLERSDRARSLNAFVH
                     LNPQAVLAQARSVDEKRKAGEMVGRLAGIPVAIKDVLCVEGEPTTCGSRMLRNFRPPY
                     DATVVAKLRAAGAILFGKTNMDEFAMGSSTENSAYGPTLNPWDPARVPGGSSGGSASA
                     VAAGLAPLALGTDTGGSIRQPAAFCGVVGLKPTYGRVSRYGLIAFASSLDQVGPFARN
                     VADAALLLSVISGPDPHDSTSVDEPIPDYVANLNTPPDSLRIGFAREYFGAGLDPEIA
                     SAIQEAIRVYEKAGAIIKEVSLPHSNYGIPAYYIVAPAECSSNLARYDGTIYGHRAAD
                     YAPKYPSEEGLPPLVRMMMASRAEGFGPEVKRRIMLGTFALSAGYADQYYNKALKVRR
                     KIRGDFDAAFQEVDVILGPTSPTPAFAIGERTADPLAMYLSDIYTITANLAGIPGISI
                     PCGFTKANLPIGLQLLAAPFAEDTLLRTARIFERATDWNLRRPGLV"
     gene            complement(2894..>3112)
                     /gene="gatC"
                     /locus_tag="VHS97_08235"
     CDS             complement(2894..>3112)
                     /gene="gatC"
                     /locus_tag="VHS97_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX3448230.1"
                     /translation="IETFTGQLNSIVDHVAQLQELDTTGVEPLAHGIEVRNVFRDDVR
                     GPALDREQALANAPKRNAEGFLVPAVLE"
BASE COUNT          560 a          924 c         1007 g          621 t
ORIGIN      
        1 gatagccgcc gcgactgcga ccgccagggc atcgcggtcc gagaccatct ggtagccgcc
       61 gcgggcgatg atagtctcgg cggcctcgcc ggtcgcgatc atctggccca ggatatcgcg
      121 cccctggttg gtgttgagtt cgcccgcttt gacgcggttg atcagaccgg cgagctggtc
      181 gggacgcacc gggaactccg ccacggtgag ctttttctcc ttgatagtcc tgagcacgtc
      241 ctgctggatc cagttgctcg ccagcttgaa ttcgcctgtg gccttggcga cggcgtcgta
      301 atagtcggcg acgtcctggc cttgctcgac cagaacgttg gcgtcgtaaa cagaaaggcc
      361 atagtcggtc tcgaaccgat gccggcgcgc cgccgggagc tcgccaatcg aggaacggac
      421 gcgttccacc cacgcggcat cgacaaccac cggaaccagg tcgggctcgg gaaaatagcg
      481 gtagtcggcg gcggtctctt tttcgcgctg aggcttcgtg acttcttcga cgtcgttcca
      541 gccgcgggtt gtctttggag cgtcattgat ggtcttgccg tcttcacgcc acttggcgta
      601 ctggcgctcg acttcgtaat ggagcgcctt ctcgacggcc cgaaagctat tcaagttttt
      661 gatctcgacg atcggtgtgg cgatcttctg gccattggcc atgatgtgga ggttgacgtt
      721 ggcatcgcag cgcagcgagc cttcctgcat ctcgcaatcg gaaacttcga ggtagcgaag
      781 cgtcagcctc agctcttcca agcagccgcg agcgtcggcc ggggttcgga tctcgggttc
      841 ggtgacgatt tccagtagcg ggatccccgc acggttcaga tcgacctcgg aaaagccgcc
      901 atcgccgtgc atcagcttgc ccgtatcttc ctccaggtgg atacgcgtca agccgcaccg
      961 gccgccgccg ccaccgtctt tacgcagtgg aatttccaga tgaccgccgc gcgaaaacgg
     1021 cagatcgtac tggctgatct gatagttttt cgggaggtcg ggatagtagt agttcttgcg
     1081 gtcccacttg gtgaaagggg cgatttcggc atggagcgca accgccgtct tcaaggcgag
     1141 atcgaatgcc ttgcggttca tcaccgggag cgtaccgggc agaccgagcg agacgggatc
     1201 cgtctgcgta ttgggaggca agccgaagtc ggtgccgctc ggcgagaaca tcttgctctg
     1261 ggtctgaagc tgaacgtgaa tttccaggcc gatgatgatt tcgtagtcca tttatccccc
     1321 attataccag ccccggccgc ctcagattcc aatcggtggc gcgttcgaag atccgggctg
     1381 tgcgcaacag cgtgtcctcg gcgaacggag cggccagaag ttgcaggcca atcggcaggt
     1441 tggctttggt gaaaccgcag ggaatgctga tgcccggaat gccggccagg ttggccgtga
     1501 tcgtgtaaat atccgacaga tacatcgcca gcgggtcggc cgtgcgctcg ccaatcgcaa
     1561 aagcgggcgt aggcgaggtt ggacctagga ttacgtcgac ctcttggaag gccgcatcga
     1621 agtcgccgcg gatcttgcgc cggactttaa gagccttgtt gtaatactga tcggcgtagc
     1681 cagccgaaag cgcgaatgtt cccagcatga tccgccgctt gacctcggga ccgaaccctt
     1741 cggcgcggct ggccatcatc atccgaacaa gcggaggtag accctcttcg ctggggtatt
     1801 tgggagcata atctgccgcg cgatggccgt agatggtgcc gtcgtaccgc gccaggttgc
     1861 tggagcactc ggccggcgca acgatgtagt aagccgggat gccgtaattg gaatgcggca
     1921 atgacacttc cttgatgatt gccccggcct tttcgtaaac gcgaattgct tcttggatgg
     1981 cgctggcgat ttcggggtcg agccccgcgc cgaaatactc gcgtgcgaac cctattctca
     2041 gagagtctgg cggcgtgttc aggttggcga cgtagtcagg gatcggctcg tcgacgctcg
     2101 ttgagtcgtg cgggtcagga ccggagatca cgctcaggag gagcgccgcg tcggcgacat
     2161 tgcgggcaaa cggcccgacc tgatcgagag agctggcgaa tgcgatcaag ccatagcggc
     2221 tgacccggcc ataggtcggc ttgagcccga caaccccgca gaacgccgcg ggctggcgaa
     2281 tcgagccgcc cgtgtcggtg ccaagagcca ggggagccag tcccgccgcg acggccgacg
     2341 ctgagccacc cgacgaacca cccggcaccc gggcgggatc ccacgggttc aatgtcggcc
     2401 cgtatgcact gttttcggtg gacgagccca tcgcaaactc gtccatgttg gtcttgccga
     2461 acaagatcgc gccggcggcc cgaagcttgg cgaccactgt ggcgtcatag ggagggcgaa
     2521 agttgcgcag catgcggctg ccgcatgtcg ttggctctcc ctcgacgcaa agaacatcct
     2581 tgatcgccac cgggatgccc gcgagccggc cgaccatctc gccggcttta cgcttttcgt
     2641 cgacggagcg cgcctgtgcc aatacggcct gtggattgag atgcacgaac gcgttgagac
     2701 ttcgggcgcg atcggatcgc tcgagcagat cgcgcacgat tgattcggac gtgatctcgc
     2761 cagcgctcag ccggtccata agcgatatgg ccgtggtgtt ttcattactc atcgaacggc
     2821 gtcctgtggc gcgcgcggcc cgaaaaaacg ggcggataag gagggtttcg cagattgggc
     2881 ctgggttggt cgatcactca aggacggccg ggacgagaaa gccctccgca ttgcgcttgg
     2941 gcgcgttggc cagcgcctgc tcgcgatcga gcgccggccc gcgcacgtcg tcgcggaaga
     3001 cgttgcgcac ctcgatgccg tgagccagtg gttccacgcc ggtcgtatcg agttcttgaa
     3061 gctgggccac gtggtctacg atggaattga gctgaccggt gaaggtctcg at
//
LOCUS       DATAQL010000477         4928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5391795, whole genome shotgun sequence.
ACCESSION   DATAQL010000477 DATAQL010000000
VERSION     DATAQL010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5391795"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VHS97_08240"
     CDS             <1..1791
                     /locus_tag="VHS97_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3448231.1"
                     /translation="VYRRCRVDVYDFRIILIDAFIAKGLTATMRRRRFLEGCGLGLTS
                     LLTAQSGNARAHAAGPGATEIAADIVIVGGGLGGCAAALAALRAGRSVILTESTDWIG
                     GQLTSQAVPPDEHPWIEQFGANASYRTLREGIRDFYRRRYPLTAEAHAQRYFNPGNGG
                     VSRLCHEPRAALAVLSELLAPYCSSRRLLVLLEHEPIRADVQGDRVRAVTVRDSKIGI
                     ERALVAPYVLDATELGELLPLAGVEHVIGAEGQTQTAEPHAPHEPAPENQQAITACFA
                     VDYSEGGDFTIDRPTEYDFWRNLVPAIKPAWPGRLLDLTYADPITLKPASRGFDPRGA
                     GVGLWVYRRLVDPKNFQPGTYPGSSGTTLVNWPQNDYWLGPLVGAQVSKADALAHAAR
                     AKQLSLSLLYWLQTECPRPDGKFGWKGLRLRADLVGTEDGLAKAPYIRESRRIKAEFT
                     VLEQHVGTETRRKLLKAQDVQAESFADSVGVGSYRIDLHPSTGGDNYIDISSLPFQIP
                     LGALIPQRVENLLPACKNLGTTHITNGCFRLHPVEWAIGEAAGAIAAYCLERGESPRA
                     VRNAAKSLSAFQARLVAQGVEIAWPRVTPR"
     gene            1899..2669
                     /locus_tag="VHS97_08245"
     CDS             1899..2669
                     /locus_tag="VHS97_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX3448232.1"
                     /translation="MTVATSNPAFSNDMFAGYEQVYGAQRTTTMTVQGTAVKTFILLA
                     IMGATATWSWNAIASGDIAFGVVAISGIGGFITALITIFKPTAAPWTAPIYAAFEGVF
                     LGAISQVIQIKYGEKYDGVGGIVMQAVSLTGGVLCVMMFVYATRLISVTDKLRTGIVV
                     ATGAIALVYVVSMVLRMFGFQVPLIYSSGLLGIGFSLFVVGLAAFNLLLDFDFIEKSA
                     HHEAPKFMEWYGAFGLMVTLIWLYMEILRLLMKLADRR"
     gene            2807..3967
                     /locus_tag="VHS97_08250"
     CDS             2807..3967
                     /locus_tag="VHS97_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448233.1"
                     /translation="MSQRKLRVGMVGGGGPANFFGAPHRRAILIDNSAELTAGALRSK
                     PAESIASAKELFMPRGYPDWQTMIKTEAAKPESERIDYITIVTPNDAHFGPADAAAAA
                     GIGVLCEKPLTTTLDEARRLQASVRAKNVPFVVAYTYTGFPMVMLARELVRGGEIGEI
                     RKVEAWYPQGWLATKLEADNQKQASWRVDPTKAGGSGCGGDIGTHAYEFVRFAAGLSA
                     VRLRARLKTFVPGRALDDDFTVLAELNNGGIATITASQVTVGVQNDNGFRVVGTTGTI
                     EWNITEHNVLKHYVGGQPLKLYRLGAEYGYFPASIKPYIRVPSGHPEGFHEALANLHR
                     TLEWTIRAGWGESVPKPFEHPGIDDGVAGMAFIEAAVASSKRDGEWVSITGG"
     gene            4087..4566
                     /gene="nikR"
                     /locus_tag="VHS97_08255"
     CDS             4087..4566
                     /gene="nikR"
                     /locus_tag="VHS97_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEX3448234.1"
                     /translation="MKDPLVRFSVAIGGELLRLFDRYRELHRYPNRSEAVRGLMRAAL
                     IEETIAHDDSEAMGVVTLIYDHHVSRIARRLTDVQHDHLDRVVTTTHVHLDDKRCLEV
                     ILLRGPAREIRDLADDLIGTKGVETGRLVLASAQAVAGRNGEGHSHHHHHPHDHAHQ"
     gene            complement(4687..>4928)
                     /locus_tag="VHS97_08260"
     CDS             complement(4687..>4928)
                     /locus_tag="VHS97_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448235.1"
                     /translation="VKALLSPWPVERPANWAARVNAPLTAKELDRVRVSIERGRPYGD
                     EKWVRETVKNLGLEQTIRPEGRPRKTSPSATEATS"
BASE COUNT          958 a         1488 c         1502 g          980 t
ORIGIN      
        1 gtctatcggc ggtgccgagt cgatgtctac gatttcagaa ttatcctcat tgacgccttc
       61 attgccaagg gactcacagc gaccatgcga cgccgacgat ttctcgaggg gtgtggcttg
      121 gggttaacca gcctgctcac ggctcaatca gggaatgcac gggcacacgc cgccggcccg
      181 ggcgcgaccg agattgcggc ggacatcgtc atcgtgggag gcgggctggg aggctgtgcc
      241 gcggcgctgg cggcgttacg agccggccgt tcggtcattc taaccgaatc caccgactgg
      301 atcggcggtc agctaacttc ccaggccgta ccccccgacg agcacccctg gatcgagcag
      361 tttggcgcga atgcatcata caggacactc agagagggta ttcgcgattt ttaccgtcgg
      421 cgttaccccc taaccgccga agcccatgcg cagcgctatt tcaatcccgg aaacggtggc
      481 gtgtcgcggc tctgtcacga gccgcgcgcc gcactagcgg tgttgtcgga attgctggcg
      541 ccttactgtt cgagcagacg gctattggtc ctcctcgagc acgagccgat ccgcgcggac
      601 gtccagggag atcgcgttcg agcagtcacg gttcgcgaca gcaagatcgg catcgagcgg
      661 gcgctcgtcg cgccgtacgt tctcgatgcc actgagttgg gggagttgtt gccacttgcc
      721 ggcgtggagc atgtgattgg agccgagggt caaacgcaaa ccgccgagcc acacgcgccc
      781 catgaaccgg cgcccgaaaa ccagcaggcg atcaccgctt gcttcgcagt cgactactcg
      841 gaaggcggag atttcacgat cgatcgaccc acagaatatg acttttggcg aaacctcgtc
      901 cccgcgatca agccggcgtg gcccgggcgt ctgcttgact tgacgtacgc cgatccgatc
      961 acgctcaagc cagcaagccg cgggttcgac cctcggggcg ctggcgtcgg attgtgggtc
     1021 taccggcggc tcgtcgaccc caagaatttt cagcccggca cctatcccgg cagctcgggt
     1081 acgaccctgg tcaactggcc gcaaaacgac tactggctcg gccccctggt gggtgcccag
     1141 gtctcgaagg ccgacgctct ggcgcatgcc gcacgggcga agcagctcag cttgtcgctg
     1201 ctctactggc tgcaaaccga atgcccccgg ccggacggta agttcgggtg gaaaggactt
     1261 cgactcagag ccgacctggt ggggacggaa gacggtctgg ctaaagcccc ctatattcgc
     1321 gagtcacgac ggatcaaggc cgagtttacc gtgcttgaac agcatgtcgg caccgagaca
     1381 cggcgtaagc tcttgaaggc acaagacgtt caggctgagt cgttcgcaga ttcggtgggc
     1441 gtcggcagct atcggatcga cctgcacccg agcaccggcg gcgacaatta catcgatatc
     1501 agctcgcttc catttcagat cccgctggga gccctgattc cgcaacgagt tgaaaacctg
     1561 ttaccggctt gcaagaatct gggaacaact cacatcacca atggctgctt ccggttgcat
     1621 cccgtcgagt gggccatcgg cgaagccgct ggcgcgatcg ccgcctattg tctggagcgc
     1681 ggggaatcgc cccgcgccgt gcgaaatgcg gccaagagtt tgagtgcttt tcaggcgcga
     1741 ctggtcgctc agggtgtcga gattgcctgg ccgcgggtga cgcccaggta gtataatttc
     1801 catgaagtga gcgaacgaac gagggcgttg cttgacggca gggcttgaca gctttttggc
     1861 tccccggccg ccaagatgaa cttgatatcc ctggaggtat gaccgtggcc acgagtaatc
     1921 ctgcgttttc aaacgacatg tttgccggat acgagcaagt ttacggcgcc cagcgcacca
     1981 cgacgatgac cgtgcaaggg acggccgtca aaacgttcat tctgctcgcc atcatggggg
     2041 ccacggcgac atggtcgtgg aacgctatcg ccagcggcga tattgcgttt ggcgtcgtcg
     2101 cgatctcggg aatcggcggg ttcatcacgg cgctgattac gatcttcaag ccaactgccg
     2161 ccccctggac ggcccctatc tacgccgcat ttgaaggtgt tttcctgggt gcgatctccc
     2221 aggtgatcca gatcaaatac ggagaaaagt atgatggcgt tggcggaatc gtgatgcagg
     2281 ccgtcagcct taccggtggc gtgctatgtg tgatgatgtt tgtttacgcg acacgtttaa
     2341 tcagtgttac cgacaagttg cggacaggca tcgttgtggc cacgggcgcc atcgcacttg
     2401 tctacgtcgt ctcaatggtg ctgagaatgt tcggtttcca ggttcccctg atctacagct
     2461 cgggcctgct ggggattggc ttcagtctgt tcgtggtcgg cctcgcggct ttcaatctgc
     2521 tcttggactt cgacttcatc gagaaatccg cccaccacga agcgcccaag ttcatggagt
     2581 ggtacggcgc cttcggcttg atggtcacct tgatctggct ctacatggag atcctccgct
     2641 tgctgatgaa gctggccgat cgacgctgaa ttaaaacgcc gcgagcggct agcccgctcg
     2701 cggtattcaa ctggtcatgt cctcccgctt aactcgcccc ttgcgatgcg cgtccgctgg
     2761 gctatagtga cctgctgagc taatcgcacc catgggagac gacaggatga gccagcggaa
     2821 gttgcgagtg gggatggtag ggggaggcgg accggcgaat ttcttcggcg caccgcaccg
     2881 ccgcgcgatc ttgatcgata actcggccga gttgaccgcc ggagctctgc gcagcaagcc
     2941 ggcggaatcg atcgcttcgg ccaaggaact gttcatgccg cggggttacc ccgactggca
     3001 gacgatgatc aagaccgagg cggcgaaacc cgagtcggag cggatcgatt acatcacgat
     3061 cgtcaccccc aacgacgccc atttcggccc cgccgacgcg gccgcggccg cgggtatcgg
     3121 cgttctctgc gaaaaaccgc tcacgacgac tctcgacgaa gcgcggcgcc tgcaggcgag
     3181 cgttcgcgcc aagaatgtcc cgttcgtagt tgcttataca tacaccggat tcccgatggt
     3241 aatgctggca cgcgagctgg tgcgtggcgg cgagatcggc gagattcgta aggtcgaggc
     3301 ctggtatccc cagggttggc ttgccaccaa actcgaagcc gacaaccaga agcaagcgtc
     3361 gtggcgggtc gatccgacca aggccggcgg ctcgggatgc ggtggagaca tcggtaccca
     3421 tgcgtatgaa tttgtgcgat ttgccgccgg cttgtcggcc gtccggctgc gggcccgctt
     3481 gaagaccttt gtgcccggcc gagcgctcga cgacgacttc acagtcctgg ccgagctcaa
     3541 caacggtggc atcgccacaa tcactgcgtc tcaagtcacg gtcggcgtgc aaaatgacaa
     3601 tggcttccgt gtggtcggta ccaccggaac gatcgagtgg aacatcaccg agcacaacgt
     3661 tctcaagcac tacgtcggag gtcagccgct aaagctctat cgactggggg cggagtacgg
     3721 ttatttcccc gcctcgatca agccatacat tcgcgtgccg tccggtcatc cggaaggctt
     3781 tcacgaggca ctggccaacc tgcaccggac gctcgaatgg accatccgag ccggctgggg
     3841 tgagtcggta cccaagccat ttgaacatcc gggaattgac gacggcgtcg cggggatggc
     3901 ctttatcgag gccgcagtcg caagctccaa acgcgacggc gaatgggttt ctattaccgg
     3961 cggatagtgg tgtgcggctg tcgtcgactg acatacgaag aagattaaga gcacgataga
     4021 gattaagatt aagaccaaac aggcaatgcc gtggattgag tcctgctctt tctctcgagg
     4081 tccccggtga aagacccatt ggtccggttc agcgtggcga tcggtggaga gttgcttcgc
     4141 ctcttcgatc gttatcgcga gctgcaccgt tatcccaacc gcagcgaggc cgtccggggc
     4201 ttgatgcggg cggcattgat cgaagagacg atcgctcatg atgacagcga ggcgatgggc
     4261 gtcgtgaccc tgatctacga tcatcacgtc agcaggatcg cgcggcggct gaccgacgtg
     4321 caacacgatc atctcgaccg ggttgtcacc acgactcacg tccatctcga cgacaagcgc
     4381 tgcctggaag tcatcctcct ccgcggccca gcccgggaga tccgtgacct cgccgacgac
     4441 ttgatcggca ccaaaggggt cgagaccggg cggctggttc tcgcctcagc ccaggccgta
     4501 gcaggacgaa acggcgaagg gcattcccac catcaccatc accctcacga tcacgcccat
     4561 cagtagacag caggcattta tcccgatgga tgcggaaaca cggacgcaat tggtttctca
     4621 atgatgcgga aacagggacg caattggttt ctcaatgaat ctggccgctt aacggaaacc
     4681 gcgcagttag cttgtcgcct cggtcgccga tgggctcgtt ttccttggcc ggccttccgg
     4741 tcgaatggtc tgctctaacc cgagattctt taccgtttcc cgcacccatt tctcgtcgcc
     4801 gtatggccgg cctcgctcga tgctcacccg cactcggtct aattccttcg cggtcaaagg
     4861 agcgttgacg cgagccgccc agttcgccgg ccgttccacc ggccaaggcg atagcaacgc
     4921 tttgaccg
//
LOCUS       DATAQL010000478         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3865278, whole genome shotgun sequence.
ACCESSION   DATAQL010000478 DATAQL010000000
VERSION     DATAQL010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865278"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VHS97_08265"
     CDS             complement(<1..1029)
                     /locus_tag="VHS97_08265"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3448236.1"
                     /translation="MARSLPWAGLAVCLTVLVAHVAYDVFAHAHSSAGGKSDTTTEAK
                     ADREPAASRSSVSESDFKLQEAKITIEPARFDRIATGVPVVGMIQVNADRQVEVRPRA
                     AGIVREVHAVLGQNVKRGESLVTLDSPDIGTARLNLRAKQRELSTARFEARWRSEIAA
                     NVAILIPELQKGINERRAALADDEEHTDTPGHDHGKRSVVPAGKGTDARLIEKRFADK
                     QLGAYRGALLQPYAEFDIASHEEQKTASLRSQNIVGEHPALVARHTREGIQAKLEAAI
                     EQVRFDAAQEKRLADQRAGQAEAAVVDAAQRLRILGVSEDIQYLLDHADEANAIARDE
                     DVTFYPIVA"
     gene            1471..1863
                     /locus_tag="VHS97_08270"
     CDS             1471..1863
                     /locus_tag="VHS97_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448237.1"
                     /translation="MSEGQLFEESAATVGSYNGITEPVGRWQVPQVCVQRGPRWGARM
                     VIARICGLVARFDERNVPVPMQHGHVIGVVGGGVPPEEMIVVDAYFVGRVMVANIVII
                     GLRQRYVNHAQNQNSDSESSRTSKCGPA"
BASE COUNT          427 a          640 c          681 g          451 t
ORIGIN      
        1 agcgacgatc ggatagaaag tcacatcctc gtcgcgggcg atggcgttgg cttcgtctgc
       61 atggtcgagc agatactgga tgtcttccga cacgccgaga attcgcaatc gttgggctgc
      121 gtcgaccacc gctgcctcgg cctgaccggc cctctggtcg gcaaggcgtt tttcctgggc
      181 agcgtcgaat cggacctgtt cgatagctgc ttcgagtttg gcctgaatcc cttcgcgcgt
      241 gtgccgagcc accaaggcgg ggtgctcgcc cacgatgttt tggctgcgca agctggccgt
      301 cttttgttct tcatggctgg cgatgtcaaa ttcagcgtac ggttgtaaca gggcgcctcg
      361 atatgcacca agctgcttgt cggcaaacct cttttcgatg agccgggcgt ccgtcccttt
      421 ccccgccggt accacagaac gttttccgtg atcgtgacct ggggtgtcgg tgtgttcctc
      481 atcgtcggcc aaagccgcgc gtcgttcatt gatccctttt tgcaattcgg ggatcaggat
      541 cgcgacattg gcggcgattt ccgatcgcca gcgagcctcg aatcgcgccg tcgacagctc
      601 gcgttgcttg gcgcgaaggt tgagccgggc ggtgccaatg tccgggctgt cgagtgttac
      661 cagcgattcg cctcgtttga cattttgacc aagcaccgca tgaacctcgc gcacgatgcc
      721 ggcggcgcgc ggacgcacct cgacttgccg atccgcattg acctggatca tccccacgac
      781 cgggaccccg gtcgcgattc gatcaaaccg cgccggctcg atcgtgatct tggcctcctg
      841 gagcttgaaa tctgactccg agaccgacga gcggctggcc gccggttccc ggtcggcctt
      901 tgcctccgtc gtagtatccg attttccgcc tgccgacgaa tgggcatgag caaagacgtc
      961 gtaggccaca tgtgcgacca agaccgtcaa acagacggcc aaccctgccc aaggaagtga
     1021 ccgtgccatc gccaaattgc tcgatggacg tcggatcaac ccgactgaca tgcgaaagcc
     1081 tcccatcatg accgccactc gagatgcgta ttaagaataa agattcaatg tctcggcatc
     1141 gactattgat gcgagttgcg gggtgctgcc cccgcggatc actgtgtggc gaccgtcatc
     1201 agcctgacac gactcgcgcg tacgcgcggc gacggacgta tgtgcgacga cgatctcggg
     1261 gaaatggccg cgccctcttg gatcccgccg tttagacgat gtgagggggg cgagaggatg
     1321 gggattgcgt cagcagttcc aacgttggaa gagagcggta cgcgaggcgc gcataccgtc
     1381 cgcgccggtt gatagaagag cggataaacg cgccaattgg tggccgagca ggagtgtttg
     1441 gtccgccgag tacgccaggc tcgcgaccga atgtccgagg gtcaactgtt cgaggagtcg
     1501 gccgcgactg tcgggtcgta caacgggatc accgagccag ttgggcgctg gcaagtcccc
     1561 caagtgtgcg tgcaacgagg gccccgatgg ggggcgagga tggtcatcgc ccgaatctgc
     1621 gggttggtcg cccgtttcga cgagcggaac gtaccagtgc caatgcagca tggtcacgtc
     1681 atcggcgtgg ttggcggagg ggtgccaccg gaggagatga tcgtggtaga cgcatatttc
     1741 gtcgggcgcg tcatggtggc gaatattgtg ataatcggcc tgcggcaacg gtacgtgaac
     1801 cacgctcaga atcagaattc cgattccgag agctcgcgaa cctcgaaatg cgggccggcg
     1861 tgacgccacg gacgtgcctc aaactcacgt gaatcaacta cctgttgcag acggcaggcg
     1921 aaccagagct cgcagccgct gagtttctcc ccattcagac attatggagc agatttatga
     1981 cccggtcaag cattgggacg atgtctgaca aagtgagaaa gagcgtctcg cgcggtcttg
     2041 acatactttg tgagataggc aaactgagct gcctttcccg cccgaaaacc gcctcgaaca
     2101 cggatgttga gggttggtca gatcatgtcg cccagacaat tcaaaggaaa cggcatcggc
     2161 ccgatcacct tctggatcgg ctggtgtctc ctgacaacc
//
LOCUS       DATAQL010000479         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_352412, whole genome shotgun sequence.
ACCESSION   DATAQL010000479 DATAQL010000000
VERSION     DATAQL010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352412"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VHS97_08275"
     CDS             complement(<1..222)
                     /locus_tag="VHS97_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEX3448238.1"
                     /translation="MCAKQIPSNDEAFTIERHGDLTIITATRSLELIEFGLEEQVADM
                     VLKPLRGQENPLIVFDLSQADNFGSMFLAL"
     gene            complement(334..561)
                     /locus_tag="VHS97_08280"
     CDS             complement(334..561)
                     /locus_tag="VHS97_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448239.1"
                     /translation="MATARLRVFPHPEEDQEDSSNPSITIRLSELYPLLAQAYRDNYV
                     WLRDFEDDELLVSNDLYEVVRAFSNSRPSAS"
     gene            995..1828
                     /locus_tag="VHS97_08285"
     CDS             995..1828
                     /locus_tag="VHS97_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448240.1"
                     /translation="MDARYARDARGVRHFVIVAVGAIPLLAGASFRSANFTVDAPSRE
                     VAQSVAERAEACRTSIARAWLGHELSAWNTPCPIRVRLTAGEAGGLTSFGFNQGRVTD
                     QSMTVEGRLDRILASAIPHEVTHTIFASYFGGPMPRWADEGASLLSEDERERLRHDQI
                     SVDLLARHGELPLARLFIIEDYPKDLMSFYGQGYSISRFLIEIGGRPRFLRFVRDGLK
                     DGWDPATRNHYQLPNVRELDRAWRSWHRVVLQAEQAPAPFDITASVNAAPANEGSSGT
                     N"
     gene            complement(1830..2810)
                     /locus_tag="VHS97_08290"
     CDS             complement(1830..2810)
                     /locus_tag="VHS97_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3448241.1"
                     /translation="MRKTSPALLRYLGALYGGGALIGCPDGQLLERLSTRNGETDRTE
                     AEMALTTLMQRHGPMVWHVCRSLVFDDHDAEDAFQASFLVLVQKAGSLRAHETIGPWL
                     YAVAYRIGLNARAASSRRQTVERAAAAKAMEVRSSGRRDQSNEIEELASLVHQEIMRL
                     PERLRSAVILCDLEGQSYRQAAVQLELPLGTVQSRLARARQRLRDRFTQKGLSPMEPD
                     RGVVPASAIIANAARLAPSASLHQACSRLCAVIATEKVGSHRLVTRSVQSLTALIDAA
                     SIRFLRRSRANLPLSGPAGDLGYPLTAEGSRRFLERADRTGYGSLRPGQG"
     gene            3135..>3729
                     /locus_tag="VHS97_08295"
     CDS             3135..>3729
                     /locus_tag="VHS97_08295"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3448242.1"
                     /translation="MRRRDFLTTMLASTAILRHRSTWAQETSGEIVFERPAAGTPHKG
                     KVLLAVQAHSDDIPLMAAGTVAKLVKEGYTGYLVRATNDDMGDAPGLGTLGSVGQHVL
                     GNERDNDEVARILGLNRVFNLNYGNHRMGDVSQNELQCRLIFLIRLLKVDTVICWDPW
                     AHDEENPDHYAIARGVEASCWMAGRDHDYPEQFAAGLK"
BASE COUNT          776 a         1129 c         1102 g          722 t
ORIGIN      
        1 gagcgccagg aacatcgagc cgaagttgtc ggcctggctc aaatcaaaga cgatcagcgg
       61 attttcctgt ccccgcaacg gcttcaaaac catatccgcg acctgctcct ccagtccgaa
      121 ttcaatgagt tccagcgaac gcgtcgccgt gatgatggtc aaatcgccat gacgctcaat
      181 agtgaacgcc tcgtcattcg aaggtatttg cttggcgcac atcaggttgg ctcaaaatcc
      241 gcgggtgcgg gattttcgta actcgacaca aaactgcggt acctcagttc attaccttag
      301 agctttacgc tcgcctagac aagcccccgc cacttatgaa gccgacggac gagagttcga
      361 gaacgcgcgc acaacctcgt aaaggtcgtt actgactaaa agttcatcgt cctcgaaatc
      421 gcgcagccaa acgtaattgt cacgatacgc ctgagccaag agcggataaa gctcgctcag
      481 tctgatcgtg atggagggat tactcgaatc ctcttggtct tcctcgggat gcgggaagac
      541 gcgcagtcgc gcggtcgcca tgggctctcc tcctccacgc gtgcctgtgc ttttccagca
      601 caattctcgc aggctctttt cctgcggttc gctcgatcca agacccgaga caaggggacg
      661 aagccgattc gggaactgca aaatcctttc cctgatccct gatccaacca gcctaaggat
      721 cggacagaga agacagcgag gatcagccaa tcccaaaaca atgcgaatga gctccgtttc
      781 gcgccgttaa aaacggtaat ctttggttcc gagtccgctc gccgacctcc ctcgtcagct
      841 cagattccgc gatgtgccgc agactgtggt caattcatta atgccggtcg cctcggtcgc
      901 caaccgcgaa aaaaggggcg acacgaaaac gggtttttga tagagttctc atccggctcg
      961 agtcggacgt atcaggccag caggagcttg gagcatggac gctcgttatg cgcgggatgc
     1021 gcgcggagtc aggcacttcg taatcgtcgc cgtgggcgca attccccttc ttgcaggtgc
     1081 ttcgtttcgt tcggccaatt tcacggtcga cgctccttcg cgtgaggtgg cgcaatccgt
     1141 ggccgaacgg gctgaggctt gccggacgag catcgcccgg gcatggctag gccacgaact
     1201 ctcggcgtgg aacacgccgt gcccgattcg cgtcaggctg accgcgggtg aggcgggcgg
     1261 tttgacctct ttcggcttca accaagggcg cgtcaccgac cagtcgatga ccgtcgaggg
     1321 tcgcctcgat cgcatcctgg catcagccat tcctcacgag gtgacacaca cgattttcgc
     1381 cagctatttc ggaggcccta tgccccgctg ggccgacgaa ggggcctcgc tcttgagcga
     1441 agatgagcgc gagcggctgc gacatgatca gatctccgtc gatctgctgg cgcggcacgg
     1501 cgaactcccg ctggcgcggc tgttcataat tgaagattac cccaaggacc tgatgagctt
     1561 ctacgggcag ggctactcaa tctcgcgctt tttaattgag atcggaggac gcccgcgctt
     1621 cctccggttc gtccgcgatg gtctcaaaga cggctgggac ccggcaactc gcaatcacta
     1681 ccagctcccc aacgtgcgcg agctcgaccg tgcctggaga tcgtggcatc gcgtcgtgct
     1741 ccaggcggaa caggcgcccg cgccgtttga catcaccgcc tcggttaacg cggcgccagc
     1801 caacgaggga tcgagcggca cgaattgact catccctgac caggcctcag ggaaccgtaa
     1861 ccggtccggt cggctcgctc caagaaccgg cgacttcctt ccgccgtcag cgggtatccc
     1921 agatcaccag ccgggcccga tagcggtagg tttgcccggg atcgacggag aaatcgaatt
     1981 gacgcagcat caataagggc ggtcaggctc tggaccgatc ttgtgaccaa tcggtgcgat
     2041 ccgaccttct cggtcgcgat taccgcgcac aaccggctgc acgcttgatg caacgaggcc
     2101 gacggcgcga gacgagcggc gttcgcaatg atggcgctcg cgggcacgac accccggtcc
     2161 ggctccatcg gacttaaccc tttttgcgtg aaccggtccc gtagtctttg gcgtgcccgc
     2221 gccaatcggc tctgaacagt tcccaagggc aattccagct ggacggcggc ttgccgatag
     2281 ctctggcctt caaggtcgca cagtattacg gctgagcgga gtcgctccgg taaacgcatg
     2341 atttcctggt ggaccaagct ggccagttcc tcgatttcat tcgattggtc gcgtcgaccg
     2401 gagctacgaa cttccatggc cttcgcggca gctgcgcgtt cgacggtctg ccggcggctc
     2461 gaggcggcgc gagcattgag accgatccga tatgcgactg cgtagagcca aggcccgatt
     2521 gtctcgtgcg cccgcagtga gccggctttt tgtacgagga ccagaaaaga agcctggaaa
     2581 gcgtcctcgg cgtcgtggtc atcgaagacg agcgacctgc atacatgcca gaccatcggc
     2641 ccatgacgtt gcatcagggt ggtaagcgcc atctcggctt cggttcggtc cgtctcacca
     2701 ttcctcgtac tgagtcgttc gagcaattgc ccatccggac agccgatcaa agcgccgccg
     2761 ccgtagagtg cgcccagata ccggagtaat gccggagaag tcttccgcat cgtggctgaa
     2821 cccgaacccg acaggcaaaa cgatggtgac gagagagcgg acattcagtt taccaaggga
     2881 taatgggccg aggcggtcca tttgttcccc aaattcgaaa aagaaagtgg gaaatcgcct
     2941 ggcgaagaag ggcgaagaag gacaagcatg ggaacctgag aacaattgct aatccgtcta
     3001 accgcttgac ggggcgtttg acgcggcact ggacgcgtgg tacacacgtg cgaaacggtg
     3061 ggtgaaacgc ccggaaccca ttcccttgcc ccggctcaat cgaccactct tccacaccgc
     3121 gcgaggagca cacgatgcga cgacgcgact tcctcaccac gatgttggcg agcacggcaa
     3181 tccttcggca ccgatcgacg tgggcacagg agacgtccgg cgagatcgtc tttgaacgac
     3241 ccgcggcggg cacgccgcat aaaggtaagg tgctgttggc agtccaggct cattctgatg
     3301 acatccccct gatggctgcc ggcacggtcg ccaagctcgt caaggagggg tacaccgggt
     3361 acttggtccg cgcgaccaac gacgacatgg gcgacgcccc aggtctgggt acgctcggca
     3421 gcgtcggcca acacgttctc ggcaacgagc gcgacaacga cgaggttgcg cggatcctgg
     3481 gcctcaatcg agtcttcaac ttgaactacg gcaaccaccg catgggcgac gtctcgcaaa
     3541 acgagctgca atgccggctg atcttcctga tccggttgtt gaaggtggat actgtgattt
     3601 gctgggatcc ctgggcgcac gacgaggaga atcccgatca ttacgcgatc gctcgcggcg
     3661 tggaagcatc gtgttggatg gcggggcggg atcacgatta cccggagcaa ttcgcagcgg
     3721 gtttgaagc
//
LOCUS       DATAQL010000480         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5086217, whole genome shotgun sequence.
ACCESSION   DATAQL010000480 DATAQL010000000
VERSION     DATAQL010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5086217"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VHS97_08300"
     CDS             <1..376
                     /locus_tag="VHS97_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX3448243.1"
                     /translation="VALADTLQTCFPPTPRTLVFGTTRDKDLEGQLRALIPLFDVIIA
                     TRYVENPRSVAPETIAAATMELADCSAHIASDPAEALDLARRLTPPSGLICVTGSLFL
                     AAEARAIVLPHVRGPSITGVVT"
     gene            440..856
                     /locus_tag="VHS97_08305"
     CDS             440..856
                     /locus_tag="VHS97_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX3448244.1"
                     /translation="MNPTFWLRTGAIWGLLAVAIGAFGAHGLKERFRLLGEQFGGLVN
                     ERPEGIFQTATQYHMYCALAIVAVGLLALSGRGGAALELAGWSFLVGSLVFSGSLYVL
                     AATGLKWLGMITPIGGVIMIAGWVALAVAAGMKSSQ"
     gene            988..>2033
                     /locus_tag="VHS97_08310"
     CDS             988..>2033
                     /locus_tag="VHS97_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEX3448245.1"
                     /translation="MAPERFRGEADGRADIYALGLTLYELLTLRPAYDSPDRLKLIER
                     IKAGDPPRPRALDPRIPRDLETIVLKAIAQDPHGRYQSADALAEDLRRFLTDEPIRAR
                     RVSWAGRLLRWGRRNKLVAGLLMSVVVSLAVGFVVSTAQWIRADRHAAREAALREQMR
                     RDLYTSDMLAVQQTWEAGHVQRMGDLLRRHIPERGQTDWRGFEWQVFWRNFQLAQPIR
                     TLPVSDNVWFLAATPKGQTMAALVYVHAPNPIDERSEVILWDAASDWKPRTFRGMPET
                     FGNAITLAPNGSVFATGSELEAKGSKPQLISIWDAASGTLLRKGPGGYDARVIIGALV
                     FSPDGKKLLWGDKDT"
BASE COUNT          343 a          640 c          658 g          392 t
ORIGIN      
        1 cgttgcactt gccgatacgc ttcaaacctg cttcccacca acgccgcgaa cgttggtttt
       61 cggaacgact cgcgataaag atcttgaggg ccagcttcga gccctgattc cgttgtttga
      121 cgtgatcatc gcgacccgat acgtcgaaaa ccctcgctcg gtcgcgcccg aaaccatcgc
      181 cgcggcgacc atggaattgg ccgactgttc cgcccatatt gcgtctgacc ccgccgaggc
      241 gctcgacctg gcacggcgac tgactccgcc gtctggcttg atctgcgtga ccggctcgct
      301 cttcctggcc gccgaagctc gcgcgatcgt tttgccccat gtgcgaggcc cgtcgattac
      361 aggtgtggtg acctgacttg gcaaacgtgg tcgcattatc tctgagaaca ctcaacaagc
      421 ttccgctgaa agacgacgca tgaacccaac attctggctg cgcacaggcg cgatctgggg
      481 cttgctggcc gtggcaatcg gggcctttgg tgcacacggg ttgaaggaac gatttcgatt
      541 gctgggtgag cagtttggcg gactggtgaa cgagcggccc gagggaatct ttcaaacggc
      601 cacgcaatac cacatgtact gtgcactggc gattgtggcc gtcggcctgc tggcgttgag
      661 cggccgcggc ggagccgcgc tcgagctggc cggatggtcg ttcctggttg gttcgcttgt
      721 cttctcggga agcctctacg tgttggcggc aaccggttta aagtggctgg gcatgatcac
      781 gcccatcggt ggtgtgatca tgatcgccgg ctgggtggcg ctggccgtgg cggcgggaat
      841 gaagtcaagc caatgaacgg cgcgactcgt ccttgatcgc ccgggctcct ggccgtccaa
      901 cattccatcg gtttttccgg tcccgctttg accgcggcgc gcggattacg agtatgggga
      961 ttctcattcc tgggaacggt ccggtacatg gcgcccgagc gtttccgtgg cgaggccgac
     1021 ggccgcgccg acatctatgc gctgggactg accctctacg aactgctgac tctcagaccg
     1081 gcgtacgact cgcccgaccg gctcaagctc atcgagcgga tcaaagccgg ggatccgccc
     1141 aggccgcggg cgctcgaccc gcgtattccc cgcgacttgg agacgatcgt cctgaaggcg
     1201 atcgcccagg atcctcatgg ccgttaccag tcggccgacg ctctggccga ggacctgcgg
     1261 cgtttcctta ctgacgagcc gatccgggcg cggcgcgtct cctgggccgg gcggctgctc
     1321 cgctggggcc gccggaacaa gctcgtcgcc ggcctcctca tgagcgtggt cgtgagcttg
     1381 gccgtcggtt tcgtcgtcag cacggcgcaa tggatccggg ccgatcgcca cgcggcgcgg
     1441 gaggcggcgc tgcgtgagca aatgcgccgc gacttgtaca cctcggatat gctcgccgtc
     1501 cagcagacgt gggaggcagg ccacgtccag cggatgggcg acctgcttcg ccgccacatc
     1561 cccgagcgcg gccaaacgga ctggcgcggc ttcgaatggc aagtgttctg gcgcaacttt
     1621 cagctggctc aaccgatccg cacgctcccg gtgagcgaca atgtgtggtt cctggcggcg
     1681 actccgaagg gccagaccat ggccgccctg gtctacgtgc acgcgcccaa tcccatcgac
     1741 gagcgcagcg aggtaatcct ctgggatgcc gcgagcgact ggaagccccg taccttcagg
     1801 ggaatgccgg agaccttcgg taatgccatc actctcgcac ccaatgggag tgtgttcgcc
     1861 accgggagtg agcttgaggc gaagggaagt aaaccccagc tcatcagcat ctgggatgca
     1921 gcgtcgggca ctctgctgcg gaagggcccg ggcggttatg atgctcgggt gattatcgga
     1981 gccctggtat tctcgcccga cggcaagaag ctgctctggg gagacaaaga cac
//
LOCUS       DATAQL010000481         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4627601, whole genome shotgun sequence.
ACCESSION   DATAQL010000481 DATAQL010000000
VERSION     DATAQL010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4627601"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..805
                     /locus_tag="VHS97_08315"
     CDS             323..805
                     /locus_tag="VHS97_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3448246.1"
                     /translation="MAHPIPSGHHAITPHLIIKGAAEAIEFYKRAFGATELSRMPFPG
                     PDGRVKLGHAELQIGDSRLFLADEFPQQGGFGPGATSPVTLHLYVTDADAAFSRAVDA
                     GAKVAMTLADMFWGDRYGRLIDPFGHHWSIAEHLEDLTPMQMQERMAATMGGPPCEDQ
                     "
     gene            1312..>2223
                     /locus_tag="VHS97_08320"
     CDS             1312..>2223
                     /locus_tag="VHS97_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448247.1"
                     /translation="MKRQLRFLVLVASFAFLVPLGVSRLKLVQAGDEPGRQDQNPDGK
                     TKAGVGQGSKTWTTAVEPKPLSRNVKKGLDWLVEHQLPGGGWGQGEESAQMRGNGSLK
                     DTPNVADTCIAALALIRSGSTPREGLYRDAIVKGVRYVRLQVDESDYQSIAVTRVQGT
                     RVQQKLGPNIDTFLASILLAEVKGRMPDEASEKAADSALHKVLAKIERHQKQDGAFDG
                     EGWAPILAQAMCGKGINRARQAGARVSDRALARAENGAKLAFQASIPPLATSASPGIS
                     SPRSAGTLGMMGGMGGAAGVELYSRAAS"
BASE COUNT          449 a          648 c          699 g          427 t
ORIGIN      
        1 tagaatattg tttttcccgc gggcgcgcct ttcgttggag atctctgtga ataaagaggg
       61 gtgggctagc gatgcgcgct caactttctt ggtccggtct tagatttccg attggcgaga
      121 cgttcttgac tctgggattc accaccccga tgaccatgat atatctgtgc tgcatgactt
      181 cctgcgaaat cgagaacgcc tcgggaaata actgtacgga cgttgacgac gtgccgataa
      241 ttcgtttgaa ttccacaacg acggcaatcc cggtcgtgtg gccaggaaat cgccggactt
      301 gtttacttga aaggagaggg ccatggcgca cccgattcca tccgggcatc atgcgatcac
      361 gccgcaccta atcatcaagg gcgcagccga ggcgatcgag ttctacaagc gggccttcgg
      421 cgcgacggag ctttcccgga tgccattccc gggaccggac ggtcgggtca agctcgggca
      481 cgccgagttg cagatcggcg actcgcgact gttcctggcg gatgaattcc cccagcaagg
      541 gggtttcggc cccggtgcca cctcaccggt gaccctgcac ctgtacgtaa cagacgccga
      601 cgccgcgttc tctcgcgccg ttgacgcggg tgcgaaggtc gccatgacct tggccgacat
      661 gttctggggc gatcgctatg gaagactgat cgacccgttc gggcaccatt ggtcgatcgc
      721 cgagcacctg gaagacctga ctccaatgca gatgcaggag cggatggcgg ccacaatggg
      781 cgggccgccg tgcgaggatc agtgagcagg atccacgccg aggccgacgc gacgttctgc
      841 gctgcgtcgg tctcggcgtc gctcacttac gaggaatctc gagcatatgg aggccccgat
      901 cgtcgcccgc gtctacgcgc tcggccgtga acgacggccg tcatcggata gcgcttgtgc
      961 ggctcttcca cacggccgca cccaccgcta agatccccta aatgcccttg cacgattttg
     1021 ggagggcgaa acaaaggtct tcgcaacgaa agcatccagc gcgcctccgt cgatcacgac
     1081 ctatcatccg ctccggccag ctagacacaa tttgtcattt ctactaataa aagtctgcct
     1141 ggagcgtcta tgagagtaga ggctgattcg ccgtcatccg aggagtcccg acgccgcact
     1201 tggggcagag gctctttgcg gagcacttgt tttccggaac tccgatgccg gcgggatcgt
     1261 ctaatgatcg ggttggacaa agtctacgca accattctga ggaggcggtc catgaagcgc
     1321 caactgcgat ttctggtttt ggtcgcgtcc ttcgcatttc tggttccgtt gggtgtgtcg
     1381 cgacttaaac ttgttcaagc gggcgatgag cctgggcgac aagaccaaaa tccggatgga
     1441 aagaccaagg ccggggtagg ccagggctca aagacctgga cgacggccgt tgagcccaag
     1501 ccgctgagtc gaaacgtgaa gaagggactc gactggctgg tggagcatca actccctggc
     1561 ggcggatggg gtcagggaga ggagtcggca cagatgcgcg gcaacgggtc tctcaaggac
     1621 acaccgaacg tggctgacac gtgcatcgcc gcgctcgcct tgatccgctc gggaagcact
     1681 ccgcgcgagg gcttgtacag ggatgcaatc gtcaagggtg tccgctacgt ccgtctgcag
     1741 gtcgacgaat ccgactatca atcgatcgcc gtcacgcgtg tccagggaac gcgggtccag
     1801 caaaagctgg gaccgaacat cgacacgttc ctggcatcaa tactgttggc cgaggtcaaa
     1861 ggtcgcatgc cggacgaggc tagcgagaag gccgcggact cggcccttca taaagtgctg
     1921 gccaagatcg agcggcacca aaaacaggat ggggcgtttg acggtgaagg ctgggcgccg
     1981 atcctcgcac aggcgatgtg cggcaaagga atcaatcgcg cgcgtcaggc aggcgcccgt
     2041 gtctccgaca gggctttagc tcgagctgaa aacggcgcaa agcttgcctt ccaggcgtca
     2101 atacccccgc tggctactag cgccagtcct ggcatctctt cgccccgatc ggcagggacg
     2161 cttggcatga tgggagggat gggcggggcg gccggggtcg agctctatag ccgtgccgcc
     2221 agc
//
LOCUS       DATAQL010000482         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5239408, whole genome shotgun sequence.
ACCESSION   DATAQL010000482 DATAQL010000000
VERSION     DATAQL010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5239408"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VHS97_08325"
     CDS             <1..579
                     /locus_tag="VHS97_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448248.1"
                     /translation="CHILDPVYTALLLTAPNSIRSEGGAPNGDNWGHDSHVRYVFPGT
                     KHTTETLTLNWYDGGKRPPDEIRALIGEQRLRNQGSIYIGSEGGLYAPYIDPPVLLPA
                     EKFKDVKLPDPGGNDHYLQFVDACRGNGKTSAPFDYSGPLTESVLLGCLATRFPKTTL
                     EWDPTALKVTNVKEANAFVRRHYRNGWEVEGL"
     gene            676..2004
                     /locus_tag="VHS97_08330"
     CDS             676..2004
                     /locus_tag="VHS97_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX3448249.1"
                     /translation="MMSLTMKTPNGKVADTGLLQGDWQERLAAIVDAMREMSLQTDPA
                     EMVRSYATRVRRLLPSSRWLSLSRRGLESPRYRITRSSTWTESVDPWKQKDRLPILEG
                     GLLGDLIYGDEPRLIDDITPLLAADDPAIEYLAGYRSLMAAPHFDHGVALNMVITLQT
                     EPAAFDPETFPEWFWVSSLFGRATHNLVLKDELKQAYDIVERELNLVAQIQRSLLPQV
                     LPKIPGVELAAYYRTSQWAGGDYYDFFPLPDGRWGILIADVSGHGTPAAVMMAITHSL
                     AHSLPGPADPPASLLGHVNRQLAQHYTASNEVFVTAFYGVYDPAQRSLTYSSAGHNPP
                     RLKRCGDGSVHALEEIGGPPLGLFEDLEYDQTTLALKPGDILAFYTDGITEAMDPSGA
                     QFGVKRLDDQLRRCGLDSTGIIRDTIEAVDQFTGGQPPEDDRTLLIAKVS"
     gene            2037..2732
                     /gene="rpe"
                     /locus_tag="VHS97_08335"
     CDS             2037..2732
                     /gene="rpe"
                     /locus_tag="VHS97_08335"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX3448250.1"
                     /translation="MSIFSDDIKLAPSILSADVAHLADQVAEAIAAGADRIHVDVMDG
                     HFVPNITFGPVVVRWLKPVTPVPLEVHLMIAEPDRYLAAFAEAGADTLIVHQEGAIHL
                     NRTVQEIHRLGLRAGVALNPATPAVLLEEILPDLELVLVMTVNPGFGGQQFLSGMLPK
                     IRRVRRMIDQVRPDCELEVDGGIEPHTAPLVVEAGARVIVAGSAVYGAAGGATAGIAR
                     LKEGLSRRVGPIV"
BASE COUNT          536 a          900 c          862 g          576 t
ORIGIN      
        1 tgccacatcc tcgacccagt ttatactgca ctcttgctca cagcccctaa ctcgattcgc
       61 tccgaagggg gcgcgcccaa cggcgacaac tggggccatg attcacatgt tcgctacgtc
      121 ttccccggaa caaagcacac gaccgagaca ctcaccctta attggtatga cggcggcaaa
      181 cgtccccccg atgagattcg ggctctgatt ggcgagcaac gtctcagaaa ccagggctcg
      241 atctacatcg gcagcgaagg aggtctctac gccccgtata tcgatccccc cgtgctgctg
      301 cctgcagaga agttcaagga cgtcaagcta cccgatccgg gcggaaacga tcactatctc
      361 caatttgtcg acgcctgccg cggaaacggc aagacatctg ctccgttcga ttactcgggg
      421 ccgctcaccg agtcggtctt actaggctgc ctggcgaccc gtttccccaa gacaacgctc
      481 gaatgggacc cgactgcgct caaggtgact aacgtcaagg aggccaacgc ttttgttcgc
      541 cgtcactacc gcaatggctg ggaggtcgag gggctctgag catcccgtct catcccacgg
      601 cgtttcctgc ctcgacggga ccggtacaat atgcgcatga cgaaatgaac catgagcctg
      661 gagtcgattg cgaccatgat gtcactcact atgaagacgc cgaacggcaa ggtcgcggat
      721 acaggtctct tgcaaggcga ttggcaagag cgcctggccg ctatcgtcga tgcgatgcgc
      781 gaaatgagcc tgcaaaccga ccctgcggag atggttcggt catatgccac acgagttcgc
      841 cgactgttgc cgtcttcccg ctggctctcg ttgagcaggc gcggtctcga atcgcctcgg
      901 tatcggatca cccggtcgag cacctggacc gagtcggtcg acccgtggaa gcagaaagat
      961 cggttgccga ttctggaagg ggggttacta ggcgacttga tctacggcga tgagccccga
     1021 ctaatcgacg atatcacgcc tcttctcgca gccgacgatc cggctatcga atacctggcc
     1081 ggctatcgct cgctgatggc ggctccccat tttgatcacg gggtcgcgct caatatggtc
     1141 atcacgctcc agaccgagcc ggccgcgttt gatccggaaa cgtttcccga gtggttctgg
     1201 gtcagcagcc tgtttggccg tgcgacgcac aaccttgtct tgaaagatga gctgaagcag
     1261 gcctacgaca tcgtcgagcg cgagctcaat ctggttgctc agattcagcg gtcgctcttg
     1321 ccccaggtct tgcccaagat cccgggcgtg gagctggcgg cgtactatcg cacgtcgcag
     1381 tgggccggag gtgactacta cgactttttc ccgttgccgg acggacggtg ggggatcttg
     1441 atcgcggatg tgagcggcca cggaactccg gcggccgtca tgatggcgat cacgcacagc
     1501 ctggcgcaca gccttccggg gcccgcggac ccgcccgcct cgcttctggg tcatgtcaac
     1561 cggcagcttg cgcaacacta caccgcgtcc aacgaggtgt tcgtcacggc gttttatggt
     1621 gtctacgacc cggctcaacg ctcgctcaca tactcgagcg ccggtcacaa cccgccccgg
     1681 ctcaagcggt gcggcgacgg ttcggtgcac gcgctcgagg aaatcggcgg cccgccactg
     1741 ggcctctttg aggacctcga gtacgaccag accacgctgg ccttgaagcc tggcgatatc
     1801 ctggcgttct acaccgacgg aatcaccgaa gccatggatc cgagcggcgc gcaattcggc
     1861 gtgaagcggc tcgatgatca gttgcggcgc tgcgggttgg actcgaccgg aatcatacgc
     1921 gacacaatcg aggccgtcga tcagtttacc ggcgggcaac cccccgagga cgaccggacc
     1981 ttgctgatcg ccaaggtgtc atgaaatcca gcgcgagcgt gggagctacg actgagatga
     2041 gtattttctc cgacgacatc aagctcgctc cgtcgatcct gtcggcggac gtggcccatc
     2101 tggcggacca ggtcgcggaa gctatcgcgg ccggtgccga ccggattcac gtcgacgtca
     2161 tggatggcca cttcgtaccg aatatcacct tcggtccagt ggtcgtgcgc tggctgaagc
     2221 cggtgacgcc cgtccctctg gaagtccacc tgatgatcgc cgagcccgac cgctacctgg
     2281 ccgcgtttgc cgaggcgggg gccgatactc tgatcgtcca ccaggaaggg gcgattcact
     2341 tgaatcgcac cgtgcaggag attcatcgat taggcttacg ggcgggagtg gccttgaacc
     2401 cggcgacgcc ggccgtgctc ctcgaggaga tattgcccga cctcgagctg gtgctggtga
     2461 tgactgttaa ccctggtttc ggcggtcagc agttcctctc cggcatgttg cccaagattc
     2521 gccgcgtccg gcggatgatt gatcaagttc gccccgattg cgagctggag gtcgatggcg
     2581 gtatcgagcc gcacaccgca cccttggttg tcgaggctgg cgcgcgggtc atcgtggccg
     2641 gctcagccgt ctacggcgcc gccggaggtg ccactgcggg catcgcccgg ctcaaagagg
     2701 gacttagccg gcgagtagga ccgatcgttt agccggcgga aacaggttgc gacgcggcac
     2761 ttcgagtttt cctttctttt tttcaagtct acaagcatcg tgcccaggcg gccgctatcc
     2821 cgggttcggt cttcctttag aatcaacttt gccctatcgt cgttccgcac attg
//
LOCUS       DATAQL010000483         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2490002, whole genome shotgun sequence.
ACCESSION   DATAQL010000483 DATAQL010000000
VERSION     DATAQL010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490002"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VHS97_08340"
     CDS             <1..567
                     /locus_tag="VHS97_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX3448251.1"
                     /translation="NELMRIEEEFFKADWARARAECGGNPKADDLWRTPVQRRADALV
                     EMAARSSLVQSDGARRPPAPLFSVLVDWHTLAGRVCELAEGIVVSPGELVPWLSYADL
                     ERAVMEPSGRVEVSSTNRLFTGATRRAIELRDRECVHAFCDVPGARCQADHALPWSHG
                     GLTTQDNGRLLCGFHNRLRNHDPPPQRE"
     gene            complement(915..1634)
                     /locus_tag="VHS97_08345"
     CDS             complement(915..1634)
                     /locus_tag="VHS97_08345"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3448252.1"
                     /translation="MSSMPIRSTPRTQAAGSGGPQVAEPTAEVGQSARKRRAIIEAAT
                     ALFLRDGYRSTSMDQIAAQAEVGKQTVYKQFEDKEQLFRAIVLGVTANSEAIITDLTS
                     ELRNGEISCLADLRVVLTDLARRYIDGVLRPNVLSLRRLVIAEAERFPDLARTYFEQA
                     PSRGIDVIADAFRSYEKSGLLEMSDTRLAAAQFAYLVLGIPQDRAQFCPAERPSPAER
                     ERLAAEAVRVFLAAYGAPSAN"
     gene            1725..>2238
                     /locus_tag="VHS97_08350"
     CDS             1725..>2238
                     /locus_tag="VHS97_08350"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX3448253.1"
                     /translation="MTVIADVQSPLRRPGFRDCSGASNVTRSLLGYLALAGPFYVTVS
                     LAQAFTRSGFDPARDEWSLLALGHQGWIQMTNLVLVGAMTAVGAIGVRRAIGRSADGG
                     VWAPRLLFAYGIALIGAGLFRADPANGFPSCTKAVGPPQASWHASLHILFGSIGFACL
                     IAACFVIARAY"
BASE COUNT          364 a          701 c          735 g          438 t
ORIGIN      
        1 aacgagctga tgcggatcga ggaagagttc ttcaaggcgg attgggcccg ggcacgggca
       61 gagtgcggtg gcaacccgaa ggccgacgac ctgtggcgta caccggttca gcgtcgggcc
      121 gacgcattgg tggaaatggc cgcccgctct tccctcgtcc agtccgacgg cgcgcgtcgt
      181 ccacccgccc ccctcttcag cgtcctggtc gactggcaca cgctggcagg ccgggtgtgc
      241 gagctcgccg agggcatcgt cgtctcaccc ggggagctgg tgccctggct gtcgtacgcc
      301 gatctggagc gcgctgtgat ggaaccctcg ggccgcgtcg aggtctcgtc gaccaaccgg
      361 ctcttcacag gcgcgacccg acgtgcgatc gagctgcgcg atcgtgagtg cgtgcacgcc
      421 ttctgcgacg tgcccggcgc acggtgccag gccgaccacg ccttgccctg gtcccatgga
      481 ggcctgacca cccaggacaa cggacgactg ttgtgcgggt ttcacaaccg gctccggaac
      541 cacgaccctc cgccccagcg ggagtagggc ggggattccg cccaggcggc tggcggtagt
      601 cgaatcaagt gtgaaatgaa gcaggcgcgc cgcgttggcg cgcctcattt catgtgcggc
      661 cgcagccacc aacggagcag ttgcgaaagc aaagggcttt ccctttaact gcgcgaccca
      721 caagttgttg ggtttgaggt ggagagtccc gcctgctccc gaacaccggg ttggagcgcc
      781 atcgatcggc actcctgagg cagtccttgg agcgatgccg gcgtgttatc ggggcacatc
      841 tgacaccgac cgccaaatcg gtcgggactt ccggccaact ggcgacgcga gtctctgttc
      901 tgttggggcg cccctcaatt cgcactcgga gccccgtacg cggcaagaaa gacccggacc
      961 gcttcggcgg cgagtcgttc gcgttcggca gggctcgggc gctctgcggg gcagaactgc
     1021 gctctgtcct gcggtatccc gaggacgagg taggcgaact gagctgctgc caggcgggta
     1081 tcactcatct cgagaagccc actcttttca taggaccgaa aggcgtccgc gatgacatca
     1141 atgccgcgcg atggcgcctg ctcgaagtag gtgcgggcga ggtcgggaaa ccgttcggct
     1201 tccgcgatga cgaggcgcct caatgacaag acattcggcc gtaggactcc gtcgatgtac
     1261 cgacgggcaa ggtcggtgag cacaacccgc aaatcggcga ggcaactgat ctcgccattc
     1321 cgcagttcgg aggtgagatc cgtgatgatc gcttcagaat tggcggtgac gccgagcacg
     1381 atcgctcgga agagttgttc tttgtcttcg aactgcttgt acacggtctg tttgcccacc
     1441 tcggcctggg cggcgatctg gtccatgctc gtgctccggt agccgtcgcg aaggaacagg
     1501 gctgttgcgg cttcgatgat ggcccggcgc tttcgggctg actggccgac ctctgccgtc
     1561 ggctccgcga cctgtggacc tccggacccg gccgcctgcg tgcggggagt cgagcggatc
     1621 ggcatggatg acatcttggc aggcctcccg gttaagacta gacagtctag gattatagca
     1681 atactataca gtctagtttc atccaagaca aaggagctga gcctatgacc gtcatcgcgg
     1741 acgtgcagag cccgctcagg cgcccgggtt tccgggattg ttcgggtgcg agcaacgtca
     1801 cccgaagcct ccttggttat ctggccttgg cggggccctt ctatgtaacc gtctccctcg
     1861 cgcaggcctt cacgcgttcg gggttcgatc cggcccgaga tgagtggagc ctgctcgccc
     1921 tcggacatca ggggtggatt cagatgacga acctcgtcct cgttggcgcc atgaccgcgg
     1981 tcggtgccat aggggtccga agggccatcg ggcggtccgc tgatgggggc gtctgggctc
     2041 ctcgcctgct cttcgcctac gggatcgcct tgatcggagc gggcctcttt cgtgctgatc
     2101 ccgccaatgg atttccgtct tgcaccaagg ccgtaggtcc gccgcaagcg agctggcacg
     2161 catcgctgca catcctgttt ggttcgatcg gctttgcttg cctgatcgcc gcttgttttg
     2221 tgatcgctcg tgcctaca
//
LOCUS       DATAQL010000484         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1574549, whole genome shotgun sequence.
ACCESSION   DATAQL010000484 DATAQL010000000
VERSION     DATAQL010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574549"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..838
                     /locus_tag="VHS97_08355"
     CDS             248..838
                     /locus_tag="VHS97_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3448254.1"
                     /translation="MNEPRPSPTSEPEILASALDWGATLAQNEQWLRRVVAARLDEPQ
                     AVDEVMQDVALAAVAQRSPLVNPARAAVWLYQLALRHVLMYRRRIGRQRSMVYRYAIR
                     KAPSETDSSPSPLAWLVSDERQQLVQAALRRLPSRDAEILVLKYAEGYGARDLAARLG
                     ATVSIIEARLHRARRRLRAELAAMAAEFEANDHDKT"
     gene            825..1583
                     /locus_tag="VHS97_08360"
     CDS             825..1583
                     /locus_tag="VHS97_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448255.1"
                     /translation="MTKLDVINPLNDDLIDQIVDGALTSAQLRAVIDMLDREPDGWKR
                     CALAFLESQCWRETFRLVGEPNPTSLQAGLTSLTENGVHTIRTGRRWLARALSASVVA
                     ASFALGWLGHGVHSGPRPGGNVVAESGEPKTEAPNTPRPQSLATTVGDGPRPTVVDGV
                     DPGNEVMMVARLRVGSADSNNSLPILAGPRIDAEWLKNQPPPVSEYGQAILQQHGYQV
                     DQRRRLISATLGDGRRVTVPIDQVQIRYTGNNPL"
     gene            1618..2697
                     /locus_tag="VHS97_08365"
     CDS             1618..2697
                     /locus_tag="VHS97_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3448256.1"
                     /translation="MNLGGVCTLASVALTCMSFPASSQQPGQRSAVSPDAYVQYSPYE
                     LFRQKTADNHLTQANKDHPQDLAWWTVADQNLGMTLTPADDALRAHLKLPGDQGLIVT
                     GLDFHSPAAQAGIEQNDILLNLENAALGKPADLEDNLKAAGDKPVSLKLLRGGKNLVI
                     QVQPRVHVGLGPVQPEAPAFWIGVSVSPIEPALRSQLKLPEKEGLLATEVFKDSPAAK
                     AEIKVHDILTKLGGKSLDSQEKLVVLVQANGGKTISLEFIREGKTQATEVTPEPRKHA
                     QGNRFKDATYSFQFVRPGAMTQFSAPLRLSDRQDFWITNQSNAPAHSSAGDAGISKRL
                     DELDAEIKQLRKAIEELSKAVATKK"
BASE COUNT          595 a          870 c          818 g          498 t
ORIGIN      
        1 agtcacccgt gcccgagccg accagcctga ccgtcatgag ccttgcgatt gccgcctttg
       61 ccgttcgcca ggttcgtcat cgcgcgaaaa gctgacggcc gaacgaacgc cttgattacg
      121 aacgggttgg ccaaaaaagc gactcccgta acggcttgtc accggcgaca atagagcaaa
      181 tccgcccagg aaaccgcaat caatcctgta aggaaagcgg tcagggcggc aactctcttg
      241 tcgaaccatg aacgagccga ggccgtcgcc cacgtctgag cccgagatcc tcgccagtgc
      301 gcttgactgg ggagcgacct tggcccagaa cgagcaatgg ctaaggcgcg tcgtggcggc
      361 acggctcgat gagccgcagg ctgtcgatga agtcatgcaa gacgtcgcgc ttgcggccgt
      421 cgcccagcga tctcccttgg tcaatccggc gcgggcggct gtctggctgt atcaactggc
      481 gctgcgccac gtcctcatgt atcgccgcag gatcggccgg cagcggtcga tggtctatcg
      541 ttacgcgatt cgcaaggcac ccagtgaaac agactcatca ccgtcgccat tggcctggtt
      601 ggtgagtgac gaacgccagc aacttgtgca ggccgcgcta cggcgcttgc catcacgcga
      661 tgctgagata ttggtcctca agtatgccga aggttatgga gcacgcgacc tggcggctcg
      721 gctgggagcc accgtatcca tcatcgaggc ccggcttcat cgcgcccgcc gccggctccg
      781 tgcggagctg gcggccatgg cagccgaatt cgaggcaaac gatcatgaca aaacttgatg
      841 tgataaatcc cttgaatgac gacttgattg accagatcgt cgacggcgcg ctgacttcag
      901 cccagctccg cgcggtcatc gacatgctcg accgtgagcc cgacggctgg aaacggtgcg
      961 ctctggcgtt tctcgagtcg cagtgctggc gcgagacgtt ccgtctcgtg ggagagccga
     1021 atccaacaag tctccaggcc ggtttgacgt ccctcacaga gaacggcgtc cacacgatcc
     1081 gaaccggccg gcgatggctg gccagggctc tttcagcgtc ggtcgttgcc gcctcattcg
     1141 cactcggatg gctgggccat ggcgtacact cagggcctcg gcccggcggc aatgtcgtgg
     1201 cagagtcggg agagccaaaa accgaggcac caaacactcc acgaccccaa agccttgcca
     1261 cgacggttgg tgatgggccc agaccaaccg tcgtcgatgg agtcgatccc ggtaacgaag
     1321 tcatgatggt ggcgcgcctg cgagtcggct cggccgattc aaataattct ctgccgattc
     1381 tggcggggcc gagaatcgac gccgagtggc tcaagaacca gccgccgccg gtttctgaat
     1441 acggccaggc gatcctgcaa cagcacggct atcaagtcga ccagcgccgg cgcttgatct
     1501 cggccacgct gggcgacggc cgccgcgtga ccgtcccgat cgaccaagtt caaatccgct
     1561 atacgggcaa caaccccttg taaccaaatg tctcaaaccg aaagaaggag atcgactatg
     1621 aatctcggcg gagtgtgcac acttgcaagc gttgctctga cgtgtatgag cttcccagcc
     1681 agttcacaac aaccgggtca gcgatccgct gtgagtcctg atgcgtatgt tcagtacagc
     1741 ccttacgaat tgttccgcca gaagaccgcc gacaaccacc tcacccaagc gaataaggat
     1801 catccgcaag atttggcttg gtggactgtc gccgatcaaa acctcggtat gaccctcacg
     1861 cccgctgacg atgcactccg ggcccatctc aagctccccg gtgatcaagg cctgatagtg
     1921 acggggctcg actttcactc gccggccgcg caagcgggaa tcgaacagaa cgacattctc
     1981 ctcaacctgg aaaacgccgc gctcgggaaa cccgcagacc tcgaagacaa cttaaaggcc
     2041 gcaggggaca agccggtttc cctcaaactt ttgcgaggtg gcaagaacct tgtcatccag
     2101 gtccagcccc gcgtccatgt cggtttggga ccggtccagc ccgaggcgcc agcgttctgg
     2161 atcggtgtgt ccgtctcacc catcgaaccg gcgctgcggt cgcagctcaa gctccccgaa
     2221 aaggaaggtt tactcgccac tgaggtattc aaggatagtc cggcagccaa agccgagatc
     2281 aaggtccatg acattctcac gaaattgggg ggcaaatcgc tggatagcca ggaaaagctg
     2341 gtcgtacttg ttcaagcgaa tgggggcaag acgatttcgc tcgagttcat tcgcgagggg
     2401 aagacccagg cgaccgaagt gacgccggag ccccggaaac acgcacaggg caacaggttc
     2461 aaggacgcga cttattcttt ccagttcgtc cgtccaggtg ccatgacgca gttttctgcg
     2521 ccgctacggc tctcggatcg gcaggacttt tggattacca atcagagcaa cgcgcccgcg
     2581 cactcctccg ctggcgacgc tggaatttcc aagcggctcg acgagctgga tgctgagatc
     2641 aagcaacttc gtaaagcgat cgaagaactg agcaaggcgg tcgcgaccaa gaaatgaccg
     2701 gccgcctcaa tcgcgcaacc tctcgtcggg aagtttttcc gggtcgggct cccgcggcgg
     2761 acgcgcgacg accgccagcc c
//
LOCUS       DATAQL010000485         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5392231, whole genome shotgun sequence.
ACCESSION   DATAQL010000485 DATAQL010000000
VERSION     DATAQL010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5392231"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1621)
                     /locus_tag="VHS97_08370"
     CDS             complement(116..1621)
                     /locus_tag="VHS97_08370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX3448257.1"
                     /translation="MMISNTYLMIFAVAMSGCVLATPLVTWVATWVGAIDRPDQFRRI
                     HKGAVPRLGGLALALGVAAGTLFTRMYEPLRQSPFGGLGANPHWSLLLAAIVILVVGF
                     IDDTRSLGPRFKLAGQALAVLTLYLGGIRIESIDVLGLNLDLGFPSARFDVMGFPVVL
                     ALPSLVVTLFWFLGCMNVWNLIDGMDGLASGVGLLVSGTLTLVAIHNENFPIAVLAVA
                     LAGSLAGFLLYNWHPACIFLGDSGSLLIGLLIGVIGVEGSMEGPSAISILFPILAMGL
                     PISDTAMAIFRRWVRNLPLSAADRRHVHHLLIGLGLNPRQAALLLYCFSGFLCGAVML
                     GVALRNEYLTLIMGISGCLVFLLVVTSRRDELANLCDDLQDRLTRGRQERQAAKLAWE
                     AIQRIDLATTEMRAYEIVDETARSLGCELVQITCGCPESGASWAKEPSAAASSRGGIL
                     SGPSAVFRLSGGDGLMISVGLPSDSVLAADIVFRYLQRLSQALVDRLERLE"
BASE COUNT          459 a          644 c          606 g          292 t
ORIGIN      
        1 agatcgttcc caagaggccc aatgcggcca gcgccgatcc ctgtcctcgc gacggaccga
       61 gctcggggtc gggcacggtg cccaaaaccg ttatcttggt catgctcgag gcggactact
      121 ccagccgctc cagccggtcg acaagcgcct ggctcaagcg ttggaggtag cggaagacga
      181 tatcggcagc cagcactgaa tccgacggca gacccaccga gatcatgagt ccgtcaccac
      241 cggagagtcg aaagacagcc gatggccccg acaagatccc cccccgcgag gacgcagctg
      301 ccgaaggctc ttttgcccat gacgccccac tctcggggca tccgcacgtg atctgcacca
      361 gctcgcatcc cagcgagcga gcagtctcgt cgacaatctc gtacgcccgc atttcggtcg
      421 tcgccaggtc gatccgctga atcgcctccc aggccaactt ggcggcttgc cgctcttgtc
      481 ttccccgcgt caaccggtct tgcaagtcgt cgcacagatt cgccagctca tcgcggcggc
      541 tggtcaccac cagcagaaaa accaggcatc ccgagatccc catgatcaat gtcaagtact
      601 cgttgcgcag ggcgaccccc agcatcacgg cgccgcagag aaaacccgag aaacaataaa
      661 gcaagagcgc ggcctggcgg gggttgagcc ctagcccgat caagaggtga tgcacgtgcc
      721 ggcggtcggc cgcggagagc ggcaggttgc gaacccagcg gcggaaaatg gccatggcgg
      781 tgtcggaaat cggtagtccc atcgccagaa tcggaaagag aatcgagatc gccgacggtc
      841 cttccattga gccctcaacg ccgatcacgc caatcaacag cccgatcaaa aggctgccgc
      901 tatcaccgag aaaaatgcag gccggatgcc agttgtacag caaaaagccc gcgagacttc
      961 ccgccagcgc cacggccaaa accgcgatcg gaaagttctc gttgtgaatc gccaccagcg
     1021 tgagcgtacc gctcacgagc aaaccgacgc cggacgcgag cccgtccatg ccgtcgatca
     1081 ggttccagac gttcatgcag ccgaggaacc agaagagggt cacgaccaag ctgggcaagg
     1141 ctagcacgac cgggaagccc atgacgtcga atcgggcgct ggggaagccc aggtcgaggt
     1201 tcaatcccag gacgtcgatc gactcgatcc ggatgccccc aaggtagaga gtcaataccg
     1261 ccaaggcctg gccggccagc ttgaagcgcg gaccaagcga acgcgtgtcg tcgatgaatc
     1321 caacgacgag gatgacgatc gcagccaata acagcgacca gtgaggattc gcgcccaacc
     1381 cgccaaaggg actttggcgc agtggttcat acatccgcgt aaagagcgtg ccggccgcga
     1441 cccccagcgc cagcgccaga ccccccaagc gggggaccgc gcctttgtga atgcgccgga
     1501 actggtcggg ccgatcgatc gcgccgaccc aggtcgctac ccaggtcaca agcggagtcg
     1561 ccagcacgca accgctcatc gccacggcaa agatcatcaa gtacgtattg gagatcatca
     1621 tcggcaagat catctcccgg tgacacgatc gatggtacga ggttgcttcc ccagcactct
     1681 catccaggta gcagcgtcag cccgcatgat tcggcatcgg cgccaacccg ctagggcgag
     1741 ctgggaagga atagtcggaa ctggccgtcg cccggtcgcg atggcagagt gagtgcggca
     1801 atggaacgct cgggaaatcg aggccgtggg ccccgaagac ggaaacgaag gcagtcgccg
     1861 acgggccgag aaccgtagac acgagcattc tcgacgacgc ggtggggcga gaggagagtg
     1921 cgtggagcgc tcgttcacga acaggatgcg gcaaagcacg gtttgtctcg tcttccatga
     1981 ctcgcatagg tgcaacgtga t
//
LOCUS       DATAQL010000486         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2949115, whole genome shotgun sequence.
ACCESSION   DATAQL010000486 DATAQL010000000
VERSION     DATAQL010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2949115"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1500
                     /locus_tag="VHS97_08375"
     CDS             58..1500
                     /locus_tag="VHS97_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448258.1"
                     /translation="MRAIVARPASWVFLILLCSYAFFQHSRDWNTASRLMLTYALVDR
                     GTVAITGLDVQTNDKAWFQGQYYSDKLPGFSFLAALPYAVARWVFRLPSHPLGGKPLR
                     FWAADYWVTLGTSGLATAAIGSILVLWARELGCRPGRATIVGLTYGLATPAYVYATLA
                     YGHQASALALFASLFLLWRRRPPRDALGLVLAGFLAAYAAVIELQVGPVSAILGLYLL
                     AQCLRGDRRPDALALFAIGAAIPTLILLAYNQFAFGSPFDMGYFHEAIKQFANVHNPG
                     NPLGLKRPDQFGRTLMALLWGRYRGLVFYAPILLLTVPGWIVLFVRRCWDLAIVTLLV
                     VVAVVLVNLFYPEWTGGWSTGPRLLLPLIPFAMLPVAALLAGKSRLTRVATIAALVLA
                     LLGGVEMLLFQGAGGRIPFAVNDPLVQFVWPHWIGPARILDRNLVSLAAPEFSARLAG
                     GWQAVQFLPLVLAQCVALLGLWRFSSSTHS"
     gene            complement(1704..1892)
                     /locus_tag="VHS97_08380"
     CDS             complement(1704..1892)
                     /locus_tag="VHS97_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448259.1"
                     /translation="MTPSAQVYWLILPLVGAISVVYSASRHESWPRIWSHSIRLGLWI
                     MGLLVAATTVLLLINTQV"
     gene            complement(1889..2428)
                     /locus_tag="VHS97_08385"
     CDS             complement(1889..2428)
                     /locus_tag="VHS97_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HEX3448260.1"
                     /translation="MNEPPVRLRNPLKAAFLAWLLPGWGHYYQGRKGKAILYASCILG
                     LYLMGMAMGEGKIVYWRWINPLNNPEKFCIYYLGQFFVGLPALPALIQGTLEHFGRDP
                     ILWGFLAEPSQTILNGLHPRLGKLVEIGTIYTTAAGLLNILAIYDAYEGPAYQEYEEE
                     STSPSAGLAASELKAEASA"
     gene            complement(2547..>3216)
                     /locus_tag="VHS97_08390"
     CDS             complement(2547..>3216)
                     /locus_tag="VHS97_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretory pathway protein ExeA"
                     /protein_id="HEX3448261.1"
                     /translation="LAGPAGLGKSTVLRKARFESQSPRRRFASIVCSPDTELLFASIA
                     ECLGQRVGREPSRLGSWRALERGVRLASLEGCQLVLAIDDCDQEVDATVGRDLESLAR
                     LGSAVNADLTIILIERNVTGAHSAVNDAWSLPICLERLTRSQAERLLAAKVEHAGNSD
                     RIFTPRAITRLHALSLGVPRKLEQLASLSLIGGAARGLEVIHPDLVDTSARRLLTDAL
                     VSSP"
BASE COUNT          584 a          975 c          960 g          697 t
ORIGIN      
        1 gcgatctggg ataaactcgc cggccgcaac tcgattgtcg gcactaggac ctgaccggtg
       61 cgtgccattg tcgcccggcc tgcatcctgg gtcttcctga ttctgctctg tagctacgcg
      121 tttttccagc actcgcgtga ctggaacacg gcgagccggc tgatgcttac ttacgcgctg
      181 gtcgatcgcg gcactgtcgc catcaccggg ctggatgtac aaaccaacga taaagcatgg
      241 ttccagggcc aatactattc cgacaagctg ccgggctttt cgttcttagc ggcgctcccg
      301 tatgcggtgg ccaggtgggt ctttcgactg ccgagccacc cgctgggcgg taagccgttg
      361 cgtttctggg ccgccgacta ctgggttacc ctgggcacat ccggactcgc gactgcggct
      421 atcggatcga ttctcgtctt gtgggcacga gaactgggat gtcgacccgg gcgagccacg
      481 attgttgggc tcacctacgg cctggcgacg ccggcctacg tttatgccac tctggcatac
      541 ggccatcagg cttcggcgct cgctctattc gcgtcgctct tcctgctttg gcgacggcga
      601 cccccgcgcg atgccttggg tctggtactt gcgggctttc tggccgcgta cgccgcggtg
      661 atcgagcttc aggtaggccc ggtgtctgcc atccttggcc tctaccttct agcacaatgt
      721 ctgcgcgggg atcgtcgtcc cgacgctctg gcgctttttg caatcggcgc cgcaattccg
      781 accctgatcc tgctggcgta caaccaattc gcctttggct ccccattcga catgggctac
      841 tttcatgaag ccataaaaca attcgccaat gtccacaatc ccggcaatcc actcggtttg
      901 aagcgtcccg accagttcgg gcgcacgcta atggccctgc tctggggtcg atatcggggt
      961 ctcgtgtttt atgcgccgat cttgcttttg actgtgcccg ggtggatcgt cctcttcgtc
     1021 aggcggtgct gggatctggc gatcgtaacc ctgctcgttg tcgttgccgt cgtgctcgta
     1081 aatctgtttt accctgagtg gaccggcggc tggtctaccg ggccccgatt gctgctccct
     1141 ttgattccct tcgcgatgct ccccgtggcc gctctgctgg cgggaaagtc gcggcttacc
     1201 cgcgtcgcga cgattgcggc cttggtgctc gctctcttgg gcggggtcga gatgttgctt
     1261 ttccaagggg cgggcgggcg aatccccttc gcggtgaacg atccactcgt ccaattcgtc
     1321 tggccgcact ggattggccc ggcgcggatt ctcgatcgca atctcgtgtc cttggcggcg
     1381 cctgaattct cggctcgact ggccggcgga tggcaagcgg ttcaattctt gccattggtg
     1441 ctggcgcagt gtgtggcgct cctgggattg tggcggttta gttcatccac ccattcttga
     1501 ccggaacggt ggtttttgcg atcgatgatt ggttcgacta ccgtcttgaa aacgatccca
     1561 ggtttctaaa acgcatcgag acagcccggc gaagcttgct tgcgggccgg ggcgttcgtc
     1621 tcgatgatgt cgagaagtga attctcgaaa gatataaata ccgctcgtac gcaggccgga
     1681 acgaccccaa cagcggccgt cgctcacact tgagtattga tcagcaacaa gactgtcgtg
     1741 gctgcgacca atagccccat gatccaaagg cccaatcgga tcgaatgaga ccagatccgt
     1801 ggccacgact cgtggcgaga tgccgaatag acgacactta tggcgccgac caagggtagg
     1861 atcagccagt agacttgggc tgatggcgtc atgccgaggc ctccgccttg agttcgctcg
     1921 ccgcaagtcc tgcggaaggc gacgtcgatt cttcctcgta ttcctggtaa gccggcccct
     1981 cataggcatc gtagatcgcc aggatattga gtaggccagc cgcggtcgtg tagatcgtgc
     2041 cgatctcgac caatttgccc agccgcggat gaagtccgtt caagattgtt tgtgaaggct
     2101 ccgccaggaa gccccacagg atcgggtcac ggccaaagtg ctctaatgtc ccctggatca
     2161 aggcgggcag cgctggcagc ccgacgaaga actgcccgag gtagtagata cagaattttt
     2221 cgggattgtt gagcgggtta atccaacgcc agtaaacgat cttcccctct cccatcgcca
     2281 tgcccatcag gtacaacccg aggatgcaag aagcgtaaag aatcgctttg ccttttcgac
     2341 cctggtagta gtggccccag ccgggtaaca gccaggccag aaacgcagct ttgagggggt
     2401 tgcggagacg aacgggcggc tcgttcatgg gactcggatc ggcctcctga tgggcatgcg
     2461 gagaaatcgg cggcccgatt atcactgtaa ggttatgcca cttcgatgct aacacgaagc
     2521 caatcggcga atctaccccg atgccctcag gggctcgaca ccaaggcgtc cgtcaggagg
     2581 cgccgcgcac tggtgtcgac cagatctggg tggatcactt ccaggccgcg ggcagccccg
     2641 ccgatcagac taagggacgc gagttgctct aacttgcgcg gcacaccgag cgacagcgcg
     2701 tgaagacgag tgattgctcg gggcgtgaag atgcggtcgg aattgccggc gtgttcgact
     2761 ttggccgcca gcaggcgttc cgcctgggat cttgtcagac gttccaggca gatggggagg
     2821 gaccaagcgt cgttcacggc cgaatgtgca ccagttacgt ttcgttcgat caatatgatc
     2881 gtgagatcgg cgttgacggc cgacccaagg cgagccaagg attcgagatc gcgccccacc
     2941 gttgcgtcca cctcctgatc gcagtcgtca atggccagaa ccaattgaca accctcgagc
     3001 gacgccaggc gtacccccct ctcgagcgcc cgccaagagc cgagccggct tggctctcgc
     3061 cccacacgtt gaccaagaca ctccgcaatt gatgcgaaca aaagttccgt atcgggggaa
     3121 cagacgatcg aggcgaatcg gcggcgcgga ctctgtgact cgaaacgcgc cttgcgcaaa
     3181 acggtcgact ttcccagccc agccggccct gccaag
//
LOCUS       DATAQL010000487         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4017626, whole genome shotgun sequence.
ACCESSION   DATAQL010000487 DATAQL010000000
VERSION     DATAQL010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4017626"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VHS97_08395"
     CDS             <1..1052
                     /locus_tag="VHS97_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448262.1"
                     /translation="LLRSRLLRRGLTPGVAALTLALSSESSAALSHDLLDRTVRSSMK
                     IALGPATAQVVSASITSPVEGVLTSMLVNTFKWAGLAVLVCGLALTGVGVMARQNAGA
                     KNEEPRPALTTLTVNAESETSAPISPENGATPKANPDGRAKDPKSQQILAKLDEPISM
                     SFNEETPLEDVLKYVRQATTTKTYSGIPIYVDPLGLQEAEKSMTSTVRNMDLEGVPLR
                     RTLQLLLEQLDLIYFVDDGLLYITSAGSEGRLRPAMHEQSPILQRAQKAERGELSLAE
                     MKELTEIMKARETIMKLTSVERDEMRDHTGGSAKQDEDAKQTRDQMNLLLKEMRQLIE
                     VLKAEKLSQKTDEGK"
     gene            complement(1132..2088)
                     /locus_tag="VHS97_08400"
     CDS             complement(1132..2088)
                     /locus_tag="VHS97_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3448263.1"
                     /translation="MILCVTLNPCLDKTLTVPVWSPGALVRGSAVREVVGGKGNNVAR
                     ALSRLGRKPRPVIFLGGTVGSRCEFLLEEDDGLDPLLVRTEAPTRVILTVLTACTSEQ
                     TAFFDPDPAVTPSEAERLFSSVEDILTSGNVDALTLSGSSPARSTHGLYSDLIALARA
                     RKVPVFLDTYGPALDGIWGFWPTAMQVNRREAAIRLRKPSVTDNDVADLLQDWHRRGI
                     TCGVVTDGPDPVFILYRGNYYRAIPPKIEVVNPIGSGDSLLAGLVDGWLDNLEPQALV
                     RHAVACAVSNALVWDAGAIEPGDVARWSEQVVIEPVSFGGRK"
     gene            2413..>3377
                     /locus_tag="VHS97_08405"
     CDS             2413..>3377
                     /locus_tag="VHS97_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX3448264.1"
                     /translation="MSGFAARLLPYALAFTSSLCIMILELVSSRLVARHVGQSLTVWT
                     SVIGIILAGICLGNVLGGRLADRVEPRRAVGPLFALGSLLTLSALWINAEIGHILPNP
                     DQINWELRTIIVIVLDFLVPATVLGMVGPVVAKMAVEQARKAGSAIGDVYFWGAMGSI
                     AGTFLCGFVLMNLAPTSTIVVLVAAALALLAAALIGEPLGLAAGIAAALLLGLGSVTP
                     LVSRLNLGAIDLGSYKLNYLAVAGTFAGLILGILGAVGLFRARSDDQALTAAVAAKSP
                     GGEFGLDKPRPKLTDLAVLAFAASMAFMALEMVAGRLVQRHLGSS"
BASE COUNT          665 a         1007 c         1040 g          665 t
ORIGIN      
        1 acctcttgcg gtcgcggctg ttgcgccggg gcctgactcc gggcgtcgcc gccttgactc
       61 ttgccctatc gagcgaatcc tccgcggctc tcagccatga tctcctcgat cggactgtga
      121 ggtcatcgat gaagatcgcc ctgggcccgg ccactgcaca agtcgtgtcc gcatcgatca
      181 cgtctcccgt cgaaggagtc ttgacttcca tgctcgtaaa caccttcaaa tgggccggcc
      241 tggccgtgct cgtgtgcgga ctcgccttga ccggcgtcgg agtgatggct cgacagaatg
      301 ccggggcaaa gaacgaagag ccgcgacccg cgctcacgac cttgacagtt aacgcggagt
      361 ccgagacatc tgccccaata tcgcccgaga atggtgcaac accaaaggct aaccccgacg
      421 gacgtgctaa ggaccccaaa tcacaacaga tcctcgccaa gctcgacgag cccatctcca
      481 tgtcattcaa cgaagagacc ccgctcgaag atgttctcaa gtacgtcagg caggcgacta
      541 caaccaagac atactcgggc atcccgatct acgtcgatcc cctcggactt caagaagcgg
      601 agaagtccat gacctcgacc gtgaggaaca tggatctcga aggcgtgccg cttcgccgaa
      661 ccttgcagct gctactggag cagctcgatc tgatttactt tgtcgatgat gggcttctgt
      721 acatcacatc ggcgggttcc gagggccgtt tgcgaccggc gatgcatgaa caatccccca
      781 ttttgcaaag ggctcagaaa gccgagcgtg gggaactctc gttggcggaa atgaaggaac
      841 tgacggagat catgaaggcc cgagagacga tcatgaaact gacctcggta gagcgagacg
      901 agatgcgcga tcacactgga ggatcggcca aacaggatga agacgcaaag caaactcgcg
      961 atcagatgaa cctcttgctc aaagaaatgc gacagcttat cgaggtcttg aaggcagaga
     1021 agctgtctca gaaaaccgac gagggcaagt gacggtttgg ttggttgtcg atcctcgccg
     1081 gcgggcggtt gggtggtaac gtaacgattc attccggatt ccagttacgg cctatttacg
     1141 gcctccgaat gacacgggct caatgacaac ttgctcgctc catcgcgcca cgtcgccggg
     1201 ctcaatggcg ccggcgtccc acacgagcgc attggaaacc gcgcaggcga cggcatggcg
     1261 aacgagagcc tggggctcga gattatcgag ccaaccgtcg accaggccgg ccagcaatga
     1321 gtcacccgag ccgattggat tgacgacctc gatcttgggc ggaatcgccc ggtagtaatt
     1381 gcctcgatac agaatgaaaa cgggatcagg accgtcggtg acgacgccgc aagtgattcc
     1441 tcggcggtgc caatcctgga gcaagtcggc cacatcgttg tcggtgacgc tcggcttgcg
     1501 caggcggatc gccgcttccc gccggttgac ctgcatggcc gtcggccaga agccccagat
     1561 accatcgagc gcgggcccgt aggtgtcgag gaaaactggg actttacgcg cgcgggccag
     1621 ggcaatcaga tcactgtaaa gaccgtgggt cgatcgggcg gggctcgagc ccgagagcgt
     1681 tagtgcatcg acattgccac tggtcaaaat gtcttcaaca ctggaaaaaa gtcgctcggc
     1741 ctcgctcgga gtgaccgccg gatcggggtc gaagaaggct gtctgctcgc tggtacaagc
     1801 ggtcaggaca gtcaggatca cgcgggtcgg cgcctctgtc cgaacgagca gcggatcgag
     1861 accatcgtcc tcctcgagca agaattcaca acgggagccg accgttcccc ccaagaatat
     1921 gacgggccgg ggctttcttc ccagccgaga cagcgcccgc gcgacgttgt tccccttgcc
     1981 acccaccacc tcgcgaacgg ccgatccgcg caccaaggcg ccaggagacc agacgggcac
     2041 cgtcagggtt ttgtcgagac aagggttgag cgtaacgcat aagatcatgt taacaatcac
     2101 cccgtcgagt gcagcgtggc cagaatgagc gcattggggc tcaccggctc taagtcttca
     2161 ttattctcgc ggtatcaccg cctggggcaa cggctcgtcc ggaagaatcc gggcccattg
     2221 ttccgcaagc aagacttgac cgccccagga catgcggcga tgcaggcatg ggcgtaaagg
     2281 tcgtcagggt aaccggttgt cgcggtgccc cgtcgtcgga tattgtgtac ccggttttcc
     2341 ttgagtcttt tttccactaa cggtttggga cgttggcgag ctgagaccgc cgaatcgggg
     2401 agtgctcaca gtatgtcagg ttttgccgcg cggttgttgc catatgctct ggcgtttacc
     2461 agtagccttt gcatcatgat cctggagctg gtttcgagcc gtctggtcgc tcgtcacgtg
     2521 ggtcaatcgc tcacggtctg gacgagcgtg atcggaatta tcctggctgg aatctgtctc
     2581 gggaacgtgt tgggggggcg tctggccgac cgtgtcgagc cccgacgggc cgtcggccca
     2641 ttgttcgcgc tggggtcact attgaccttg agcgcgctct ggatcaatgc cgagattggt
     2701 cacattctcc ccaaccccga ccagatcaac tgggagctac ggacgattat cgtgatcgta
     2761 ctcgacttcc tggtgccggc gacggtgctg ggtatggtcg ggccagttgt ggccaagatg
     2821 gccgtcgagc aggctcggaa agccggcagc gcgattggcg acgtatattt ttggggcgcg
     2881 atgggctcga tcgccggcac gtttctctgc gggttcgtgc tcatgaatct ggcgccgacg
     2941 tcgacgatcg tcgtcctggt cgccgccgcc ctggcgctct tggcggccgc tctgattggc
     3001 gaaccgttgg gactggctgc cggaatcgcc gcggccctct tgctgggact tggctcggtc
     3061 acgcctctgg taagccggct gaatctcggt gccattgatc tgggctctta caagctgaat
     3121 tacctcgcgg tagccggcac attcgcgggg cttatcctgg gaatcttggg ggcagtcggg
     3181 ctcttccgcg cccgtagcga cgaccaggcg ctcacggcgg ctgtcgccgc caaatcgccc
     3241 ggcggcgaat tcggcctcga caagccgcga cccaaactga ccgatctcgc ggtccttgcc
     3301 ttcgcggcga gtatggcgtt catggccctc gagatggttg ccggccgctt ggtccaacgg
     3361 cacctgggct cgagcat
//
LOCUS       DATAQL010000488         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4017702, whole genome shotgun sequence.
ACCESSION   DATAQL010000488 DATAQL010000000
VERSION     DATAQL010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4017702"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VHS97_08410"
     CDS             <1..217
                     /locus_tag="VHS97_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448265.1"
                     /translation="AAEAIAARCLRRDSNKIKHGKPADLPKKRAVRTKPRTTGTDAAQ
                     TVTESAELVNAGSGPNKRDGPDLTGSL"
     gene            541..1569
                     /locus_tag="VHS97_08415"
     CDS             541..1569
                     /locus_tag="VHS97_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3448266.1"
                     /translation="MAQPADQSVKQPLSENDVQAIDHLRDAYARLRKELGKVIVGQRD
                     VIERLSICLFARGHALLMGVPGLAKTLMVNKLAETMSLKFSRIQFTPDLMPMDITGTD
                     ILQDTAAGRREFEFVHGPVFANIVLADEINRAPPKTQAAMLEAMQEQNVTVVGKVFHL
                     EPPFLVLATQNPIEQEGTYPLPEAQLDRFMFLIELEYPSEAEEIQIARTTTGEALPRL
                     DHLMTPAEIIKHQSLVRRVPVPDHIYSYAARLVRKTRPGGSTAPSWLKPLVSWGAGPR
                     AVQYLILGAKARAALLGSYMVRLEDVQEAAAPVLTHRIITTFAAQAEGIDAKKIVRRL
                     VEETDSSA"
     gene            1785..2696
                     /locus_tag="VHS97_08420"
     CDS             1785..2696
                     /locus_tag="VHS97_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3448267.1"
                     /translation="MFPDRPNRSFLDHGVLSRLAGFPLFARRPMQGSVSGRHPSPHRG
                     SSVEFAEYRKYVPGDDLRRLDWRAYGRSDRFFVKEFEADTNLRCCLVVDTSGSMGFGS
                     AAVSKLEYARRIAGALGHLALQQGDAVGLSCVAGGIVRNIPPRRNPSHLMTLFDVLEQ
                     TEPRGQTQLVPVLHELAETISQRALIVIISDLFVEPDELRSCFQHLRFRRHDAAIFHL
                     LDPQELGFDFRRPMRFLDMEGGPSIFAEPNEIAERYHKAVSGYLAAIHQVVLESAVDY
                     HRVTIDESYEQVLIRFLVGRTRQKGVR"
     gene            2693..>4196
                     /locus_tag="VHS97_08425"
     CDS             2693..>4196
                     /locus_tag="VHS97_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX3448268.1"
                     /translation="MSFLQPMLLAALPLVALPIIIHLINQRRYQTVKWAAMMFLLAAN
                     RMSRGYARLRQWLIMTMRMAALAGLIFAVSRPLSGGWLGLAAGGRADTTIIVFDRSPS
                     MQQVGADARGSKLETGLRQLVATLETLSSARWVLIDSNTNKPRELEKIADLLTTPSAS
                     PSSASADLPAMLLAALEYVKSNKTGQTEIWICSDLRANDWNSESGRWQALREGFTGVP
                     QTVRFHLLAYQDTAPLNRWVRVTDVRRQKAGDAAELLVSLKVSREGPASASESIPVQF
                     EIDGARSEVTIELAGGQGELKDHRIPLEKGRDRGWGRVSIPADANPADNDFWFVFEQP
                     APRRTVVVVEDPQAARSLQLAASISPDPLLKCTAEVLQASQLASVDWDSVSLLLWQAP
                     VPDAAVGKEVQTFIDRGGSAIFFPARLPGTAEFLGARWTSWAELKAEAPVEGWRGDQD
                     LLAHTASGAPLPVGQLQVRKACGLLGEITPLATLRGGSPLLARLNTPRGSA"
BASE COUNT          780 a         1323 c         1314 g          779 t
ORIGIN      
        1 ggcggcggaa gccatcgctg cccgctgttt gcgccgagac tcgaacaaga tcaagcacgg
       61 caaacccgcg gacctgccca agaagcgagc ggtacgcacc aagccgagaa cgacggggac
      121 agatgcagcc cagacagtaa ctgaatcggc cgagctggtg aacgctggct caggaccaaa
      181 caagagggac ggcccagatc tgacagggtc gttgtaatgg tgtgtagcgg tgccgagccg
      241 cgagaaatcg cggcgagggc caccaacatg ccgtggttac cggtgggtgg gcctcacgat
      301 cccgaggatc gctcatgccg ccgttatcgg tgggcctcac gatcccgagg atcgttcggc
      361 gccaccctac gaaggcacgt cctagcaatt tagttcgagg ctctggttgt cgcgcgcacc
      421 cacaggctag aatgtcggaa acttgtcagg ggctatcact cgaatccccg cagcttcgct
      481 cgcgatcggt agtaaagggc gcgatcccag ccttcatgga acgacaacgg gagtgtttcg
      541 atggcgcagc cggcagacca atccgtaaaa cagccgctca gtgagaatga cgtgcaggcg
      601 attgaccacc tcagggatgc gtacgcccgg ttgcgcaaag agctgggcaa ggtcatcgtc
      661 ggccagcgcg acgtcatcga gcggctgtcg atctgtctgt tcgcgcgcgg ccatgcgcta
      721 ctgatggggg tgcccgggtt ggccaaaacc ttgatggtca acaagctcgc ggaaacgatg
      781 tcgctcaagt tcagccgcat ccagttcacg cccgacttga tgccgatgga tatcacggga
      841 accgacatcc tccaggatac ggccgcgggc cgccgcgagt ttgagttcgt gcacggccct
      901 gtattcgcca atatcgtcct ggctgatgag atcaaccgcg ccccacccaa gacccaggcc
      961 gccatgctcg aggccatgca agagcaaaac gtgaccgtgg tcggcaaggt gtttcacctc
     1021 gagccgccgt tcctcgttct ggccacgcaa aacccgatcg agcaggaagg gacgtacccc
     1081 ctccccgagg cccagctcga ccggttcatg ttcttgatcg agctcgagta cccatccgaa
     1141 gccgaagaga ttcagatcgc ccgcacgacc accggcgagg ccttgccgcg cctcgatcat
     1201 ctgatgacgc cggccgagat catcaagcac cagagcctgg tccggcgcgt tccggtacct
     1261 gaccacattt actcgtacgc ggcccggctg gtccggaaaa cgcgcccggg cggctcgacg
     1321 gccccctcat ggttgaagcc gcttgtgtcg tggggagccg gaccacgcgc ggtccagtat
     1381 ctgattctag gcgccaaggc acgcgcggcg ttacttggta gttacatggt gcggctggag
     1441 gatgttcagg aagccgccgc ccctgttctc acccaccgca tcatcaccac cttcgccgct
     1501 caggccgaag gaatcgacgc caaaaagatc gtccgccggc tagtggagga gacggattct
     1561 tcagcttagt ccttggtcct tggtccttgg tccttggtcc ttggttagtg gctggcggtg
     1621 gctacttttg ctgacgagct tctggtcacc atcagctgag catgaatcac ggaccacgga
     1681 ccccagaact ctgacgggtg acaacgaaaa ggactgagca caggaccacg gacgaaggac
     1741 caaggaccaa ggaccaagga ccaaggacca aggacaatcg agaaatgttc cccgaccgtc
     1801 cgaatcggag ctttctggat cacggcgtgc tgtcgcggct ggccggcttt cccctcttcg
     1861 cccgacggcc gatgcagggg agcgtttcgg gtcgccatcc cagtccgcac agaggatcga
     1921 gtgtcgagtt tgccgagtat cgcaagtacg tgccggggga cgatctgcgc cggctggatt
     1981 ggcgcgctta cggccggtcg gatcggtttt tcgtcaagga gtttgaggcc gataccaacc
     2041 tgcgctgttg cctcgtcgtc gataccagcg gctcaatggg gtttggctcg gccgccgtct
     2101 ccaagctgga atatgcccgg cggatcgccg gggcattggg ccacttggcc ctccagcaag
     2161 gcgatgcggt cggtttgtcg tgcgtcgccg gcgggattgt gcgcaatatc cccccccggc
     2221 gcaatccgtc gcatttgatg acgctattcg atgtactgga acaaacggag ccccgcgggc
     2281 aaacccagct tgtgccggtc ctgcacgagc tggctgagac catctcccag cgagccctga
     2341 tcgtgatcat ctcggatcta ttcgtcgaac ccgacgagct gcgctcttgc tttcaacacc
     2401 tgcggttccg gcgccatgac gccgcgatct ttcatttgct ggaccctcag gaattgggct
     2461 ttgactttcg gcggccgatg cgctttctcg acatggaagg gggaccgtcc atcttcgccg
     2521 agcccaacga gatcgccgaa cgctatcaca aggccgtctc cggatatctg gcggcgatcc
     2581 accaggtcgt tctcgagtcg gctgtcgact atcaccgggt cacgattgac gaaagctacg
     2641 agcaggtgtt gattcgtttc ctggtgggac gcacccgtca aaagggggtg cgatgagctt
     2701 cctccaaccg atgctcttgg cggcgctgcc gctggtggcc ttgccgatca tcattcacct
     2761 gatcaatcag cgccgatatc agacggtgaa atgggcggcg atgatgtttc tcctggccgc
     2821 caaccggatg tcgcgaggct atgcccgact gaggcaatgg ctgatcatga cgatgcgcat
     2881 ggccgccctc gccggcttga tctttgcggt cagccgacca ctttcggggg gctggctggg
     2941 gctggccgcc ggcggccgtg ccgacaccac catcatcgtc ttcgaccgct cgcccagcat
     3001 gcagcaagtc ggggcggacg cgcgcggatc caagctcgag accggcctgc gacaactggt
     3061 tgcaacgctc gaaaccctga gctcggctcg gtgggtcttg atcgatagca acacgaacaa
     3121 gccgcgcgag ctcgaaaaga tcgccgatct cttgaccacg cccagtgcct cgccctcaag
     3181 cgcatccgcc gacttgccgg cgatgctgct cgcggctctc gaatacgtga agtccaacaa
     3241 gaccgggcag accgagatct ggatctgctc cgatttgcgt gccaacgact ggaactccga
     3301 gagcggccgc tggcaggcac tgcgcgaggg attcaccggt gtgccgcaga cggtccgctt
     3361 tcatttgctg gcttaccagg acaccgcgcc tttgaaccgg tgggttcgcg tgaccgacgt
     3421 ccgccgccag aaggccggcg acgcggccga gctcttggta tcgctcaaag tgtcacgcga
     3481 agggccggcg agtgcgagtg agtcgatacc ggtccagttc gagatcgatg gcgcgcggtc
     3541 cgaggtgacg atcgagttgg ccgggggaca gggtgagctc aaggaccacc gcatcccgct
     3601 cgaaaaaggt cgtgaccgcg gctggggtcg cgtgtcgatc ccagcggatg ccaacccggc
     3661 ggacaacgat ttctggttcg tcttcgaaca gcccgccccc cggcggacgg ttgtagtcgt
     3721 cgaggatccg caggccgccc ggtcgttgca actggccgct tcgatctcgc cggacccact
     3781 tctgaagtgt accgccgaag tcctgcaggc cagtcaactg gcgtccgtcg actgggattc
     3841 agtatcgctc ctcctatggc aggctcccgt tcccgacgcc gcggtaggta aagaggtgca
     3901 gaccttcatc gaccgcggcg gttcggcgat ctttttcccc gcccgtttac ctggcacggc
     3961 cgagtttttg ggcgcgcgct ggacgtcgtg ggcggagctc aaggccgagg cacccgtcga
     4021 gggctggcgt ggcgaccagg acttgctggc ccacacagcc agcggcgctc cgttaccggt
     4081 cggccagctc caagtcagaa aggcgtgcgg gctcttgggt gaaatcactc ccttggccac
     4141 gctacggggc ggatcgcctc ttttggcgcg gctcaacacg ccgcgcgggt cggcct
//
LOCUS       DATAQL010000489         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1729046, whole genome shotgun sequence.
ACCESSION   DATAQL010000489 DATAQL010000000
VERSION     DATAQL010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729046"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VHS97_08430"
     CDS             complement(<1..292)
                     /locus_tag="VHS97_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448269.1"
                     /translation="MRELAFSLPYLKFNFVLSIMLSTSLGFPQESMIALMDDSSMIFI
                     AEICLTRERRGPQAKFARYPSIYILDVDEDREEALKHSHFTIARSTPLTFELV"
     gene            469..1452
                     /locus_tag="VHS97_08435"
     CDS             469..1452
                     /locus_tag="VHS97_08435"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3448270.1"
                     /translation="MRRGVFGLFILGMVAGAFGALAEGQTIERDTTITGPRGRTIQRD
                     VEIKRGPGTIDRQVQIKRPGGTLERSVQVQRSPVMGPWRPPIGGPWARPVWVPRPVVV
                     GPPAVGFGLLAAPMLNFSFGGGGGGIGGGVGGPGPGMGGPGGPGPGGPAAGTPPPPDQ
                     TALLTQRLQSIVPSHRKDAAYALGQSGDPRAIPSLVHVLKYDLSKDVRVASAIALGEI
                     GGSESAVALERCAIYDKKDDVRKAATTALERLNTKAKNAPPVSSEVAPSNAMPAPPAT
                     SGPSPFRQSPPPALEPPAGEPSQGPGDPPASPQPPPPPTPVASGPGSAGLR"
     gene            complement(1490..3277)
                     /locus_tag="VHS97_08440"
     CDS             complement(1490..3277)
                     /locus_tag="VHS97_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3448271.1"
                     /translation="MSRLTVRSKARMSARPTVLDSTRSLADPYGRTSLAVIVAVMLCQ
                     IAFLVFGCDWDFCNDEAEYWAWSRRLDWSYYSRGPLIAWVIRLATELLGGLSVKLTGS
                     LMFAARLPAVLLGGLTAWGVFRLTSLTTGQRRAAMMSVVLLPAIPILAIGGVVITSDT
                     PLVCCWTWAAVWTYRGARSDDLRSWVAAGLIGALGVWAKYSFLAFPASVGLFLLTSRV
                     HRRHLLRPGFWTMVLLCLGMGLAPILIWNFRHGWAGAGQLADRVGLSSRATWASIGPV
                     LTFLGGDAAALGGVWWIAGIAAVVAAIARVIGRSRSVAAAEPDETAGVYDSGLIYLLC
                     LWGVIWTACLAASLLGENEANWMVPGYISLVILIALRVDRVFADGGVRKWVYTAAWCL
                     CLAVVIAIHHVEWFYPALARYLPEPTPRWPVPLRVYEPTARMRGHQTLARAVEKTMEA
                     VDAKGVSPYVLTPTYGLASTLSFYLPGQPETYCLSWNYGMTPQPVNQHDLWHPNPRHD
                     PAAFRARAAIVVEDANMPPNWAYHMFQKRVFGSLESTERVTVRERGVIVGAWDISICH
                     DYRGVADYQQFVPGRRRLAGHQQISPVRR"
     gene            complement(3508..>3901)
                     /locus_tag="VHS97_08445"
     CDS             complement(3508..>3901)
                     /locus_tag="VHS97_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX3448272.1"
                     /translation="GGDQDLLGFGTVADGEWVVARLRSDTAMDRLAPEHSSDWRSLGV
                     SVLHVLVLNELLADKGTASCRYVHSIAEVLADTAARNCDLACLVPPAQMGHVESIASN
                     LETMPPKSTYFYPKLLTGLVLNPIRRLD"
BASE COUNT          817 a         1188 c         1225 g          671 t
ORIGIN      
        1 tgaccaattc aaaggtcaac ggagttgacc ttgcaatcgt gaaatgggaa tgcttcaacg
       61 cttcctccct gtcttcatcg acgtcaagga tatagatgga cggatatctg gcaaactttg
      121 cctgaggacc acgtcgttct ctagttaggc agatctctgc tataaatatc atagacgagt
      181 catccattaa cgcaatcata gattcttgag gaaagcccag actggttgat agcatgatgc
      241 tgagcacaaa gttgaactta agatacggaa gtgaaaacgc caactctcgc atggaatatg
      301 agaccacatt tagacttagg gtaattgccc ttgcatttga ccttcacaag attgtcgcgg
      361 aagagcacaa tccacggcca tcattttggt cgaggtgaga actgattcac gcagaaaaat
      421 gcgttttagg ggatactccg aacagggaag ggagttgcga tgatcaggat gagacgagga
      481 gtgtttgggc tttttatttt ggggatggtc gcgggagcgt tcggtgcgtt agcagaaggc
      541 cagacgatcg agcgcgacac tacgatcacg ggtccacgtg gccggacgat ccagcgcgat
      601 gtcgagatta agcgcgggcc gggcacgatc gatcgacagg tccagatcaa gcggcctggc
      661 ggaacattgg agcgttccgt tcaagttcag cgctcgccgg tgatgggtcc ttggagaccg
      721 ccgattggcg gtccttgggc tcgtcctgtc tgggtaccgc ggccggtcgt ggtcggcccg
      781 ccggcggttg ggtttggctt gctagccgcc ccgatgttga acttctcgtt tggaggtgga
      841 ggtggtggga tcggaggggg agtagggggc ccgggccccg gaatgggggg acccggcggg
      901 ccggggccgg gcggacccgc ggccggaact ccgccgccac ccgatcagac ggccctcttg
      961 acccagcgct tacagagcat tgtccccagc catcggaagg acgccgccta tgcgcttggt
     1021 cagtcgggag acccgcgcgc gattccttca ttggtccacg tcctcaaata cgacttgtca
     1081 aaggacgtgc gggttgcctc ggcgatcgcg ctcggtgaga tcggcggctc ggaatccgca
     1141 gtcgcgctcg aacgctgtgc tatctacgat aagaaggacg atgtccgcaa ggcagcgacc
     1201 acggctttgg agcggttgaa taccaaggcc aagaatgcgc cgccggtttc gtcggaggtc
     1261 gcgccgtcca atgcgatgcc cgcgcccccg gcgacgtccg gtccgtcgcc tttccgtcaa
     1321 tcgcctccgc cggccctcga gccaccggct ggtgagccgt cgcaaggtcc gggcgatccg
     1381 cccgcctcgc cacaaccccc gcctccaccg acgccggtcg cgtccggacc aggaagcgcg
     1441 ggcctcaggt agactgcctt gtgagaaaac ggaatcgtac ccggtcgatt catcgccgga
     1501 ccggcgagat ctgctggtga ccggccagcc ggcgccggcc cggcacgaac tgctggtaat
     1561 ccgccacgcc gcggtagtcg tgacagatcg agatatccca tgctcccacg atcactccgc
     1621 gctcacgcac ggtcacgcgc tccgttgact caaggctgcc gaacacccgc ttctggaaca
     1681 tgtgataggc ccagttggga ggcatgttgg cgtcttcgac cacaatcgcc gctcgggccc
     1741 ggaaggcggc tgggtcgtgc ctcgggttgg gatgccagag gtcgtgttgg ttgacgggct
     1801 ggggagtcat cccataattc cagctcaggc aatacgtctc gggctgaccg ggtaggtaaa
     1861 acgagagcgt cgacgccaat ccataggttg gagtcaagac gtaaggagac acgcctttgg
     1921 cgtcaactgc ctccatggtt ttttcgaccg ctctggctag ggtctggtgc ccacgcattc
     1981 gggccgtcgg ctcatacact cgcagcggca caggccatct tggggtaggt tcagggaggt
     2041 agcgggcgag cgccgggtaa aaccactcga catgatggat cgcgatcacc acagcgaggc
     2101 agaggcacca ggcggcagtg tagacccatt tccgcacgcc cccgtcggca aagacccggt
     2161 caacgcgcaa cgcgatcagg atcacgagcg agatatagcc tggcaccatc cagttggcct
     2221 cgttctcgcc cagcagactg gcggccaggc aggccgtcca gatcactccc cagagacaga
     2281 gaaggtagat caagccggaa tcgtagacgc cggcggtctc gtccggctca gcggcggcga
     2341 cagacctcga ccgtccgatg acccgagcaa tcgcagcgac gacggctgcg ataccggcga
     2401 tccaccagac tcctccaagc gcggccgcgt cgccgccaag aaaggtgagg acgggcccga
     2461 tactcgccca ggttgcccgc gatgataggc ccacgcggtc ggccaactgg ccggctcctg
     2521 cccacccgtg gcggaaattc catatcaaga tcggggcgag gcccatccca aggcagagca
     2581 gcaccatcgt ccagaagcct ggccggagca agtggcgacg atggacccta ctggtcaaga
     2641 gaaagagacc caccgacgcc gggaacgcca gaaaggagta tttcgcccac acacccagag
     2701 cgccgatcaa gcccgcggcg acccacgaac gcagatcgtc ggagcgagca cctcgatacg
     2761 tccagaccgc ggcccaggtc caacaacata ccaggggcgt gtcgcttgtg ataacgacgc
     2821 caccgatcgc caggatcggt attgccggca gtagcacgac cgacatcatc gcggcccgcc
     2881 gttggcccgt cgtcagcgat gtcaggcgga atacgcccca cgccgtcagt ccgcccagga
     2941 gaacggctgg cagccgagcc gcgaacatca gcgaacccgt cagcttgacg ctcagtccgc
     3001 ccagcaattc ggtcgcaaga cggatcaccc aggcgatcaa ggggccacgg gaatagtagc
     3061 tccaatccag gcgacgcgac cacgcccagt attccgcctc gtcattgcag aaatcccaat
     3121 cgcagccgaa cacgaggaag gcgatctggc atagcatcac ggccacgatc accgccagcg
     3181 acgtgcgtcc atagggatcg gcgagcgagc gtgtcgaatc gaggacggtc ggacgagcag
     3241 acattcttgc cttgctgcgc acggtcaacc ttgacatctg accctaaact cgtccgcgaa
     3301 aaagagcggg aagcgcggat cggtcctctc gggagccgac cgctgggttc gaccgatgtt
     3361 gcgccgcgca acaaaccgtg tcgaacttcc tgtgtctctc ttatcggcgg ttcgagccgt
     3421 cgactcgatg gaaaatgagc agcgccgcca tgtgcacatg cggcaatctg actggtagaa
     3481 aggaagtgaa atccaaccgc gcgagcctca gtcgagccgg cggatcgggt tgagcacgag
     3541 tcccgtcaag agtttgggat agaaataggt gctcttggga ggcatggttt cgaggttgga
     3601 ggcaatgctt tcaacgtggc ccatctgggc gggcgggacg agacaagcga ggtcgcagtt
     3661 gcgtgcggcc gtgtcagcga gaacctcggc gatcgaatgg acgtagcggc aggacgcagt
     3721 ccccttatcg gcgagaagtt cgttgagcac gaggacgtgc aaaacgctca cgcccagact
     3781 gcgccaatcc gagctgtgct ccggcgcgag gcggtccatc gcggtgtccg atcgcagcct
     3841 ggcgacgacc cattcgccgt cggcgaccgt tccaaagccg agcagatcct gatcgccccc
     3901 c
//
LOCUS       DATAQL010000490         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_810521, whole genome shotgun sequence.
ACCESSION   DATAQL010000490 DATAQL010000000
VERSION     DATAQL010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810521"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..>1509)
                     /locus_tag="VHS97_08450"
     CDS             complement(242..>1509)
                     /locus_tag="VHS97_08450"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3448273.1"
                     /translation="FHPYLGGEAVKRIRERVAGLDVHRDTVVACCQLRQPDRSVEVTK
                     QSFATTAKGLGELAAWLRDTGVDTVAMEATGVYWKPVYYCLEGLFDELWLCNAQHVKN
                     VPGRKTDLSDAEWLADVAAHGMVRPSFVPPPEIRELRDVTRYRKTQVEARAKEIQRLE
                     KTLQDAGIKLSSVASKIWTKSSREMIEAMIEGERDPKVLAQMAKSRLRAKIPQLEEAF
                     RGRFGSQHALVCRQVIEHIDFLDRSIATLTADVTDRLCPFESAVTILCSIPGVSRTTA
                     EVMIAEMGVDMTQFPTSGHLCAWAGVAPASHESAGKRQPVGTRHGSTWLQRALVEAAR
                     GASRTKGTYLCAQYSRLARRRGSNKATIAVAHTILDVCWHLLRNGALYDDPGARFFEL
                     RHDPAVEAKRLQRRIEALGFEVSIAPAAA"
BASE COUNT          257 a          476 c          487 g          289 t
ORIGIN      
        1 cgtcatcgcc gcgccagcct gctcagtgat gccacgtgac acacgtccag ctcctcggag
       61 gcatgagcaa tcaatgtgcc gaccaggctt cccggctctc cgcgcaccag ggtaccgttc
      121 accgcttcgg caaccgggct cgggatactg gctggggtga aatgcgttgt gcccgacgcg
      181 tcgctggtcg cgcgcgacga ggtgcctttt tggggctctg agttggtcgt tggtgaaaga
      241 tctatgcggc agcaggtgcg atgctgactt cgaatccgag agcctcgatc cggcgctgga
      301 gccgcttcgc ctcaaccgcg ggatcgtggc ggagctcgaa gaatcgagcg ccagggtcgt
      361 cgtagagagc accgtttctg agcaagtgcc aacagacgtc caagatggtg tgggcgacgg
      421 cgatcgtggc cttgttggac cctctgcgtc gggcgagcct ggagtactga gcacacaggt
      481 aggtcccctt ggtgcgcgac gcccccctgg ctgcttcgac aagggcgcgc tggagccaag
      541 ttgagccgtg gcgcgtgccg acgggttgac gtttgccggc cgactcatga ctggccgggg
      601 cgaccccggc ccatgcgcac aggtgccccg aggtcgggaa ctgggtcatg tcgaccccca
      661 tctcggcgat catcacctcg gcagtggtcc tcgagacccc gggaatcgag cacaagatgg
      721 tgaccgccga ctcaaaagga cacaggcgat ccgtcacatc ggctgtgagc gttgcaatag
      781 agcggtccag aaagtcgatg tgctcgatca cctgacgaca gacgagggca tgctgggagc
      841 cgaagcgacc gcggaatgct tcttcgagct ggggaatctt ggctcgcagt cgcgacttgg
      901 ccatctgggc caggactttg gggtcccgct ctccctcgat catcgcctcg atcatctccc
      961 gcgacgactt ggtccagatc ttcgacgcca ccgaggacag tttgatgccg gcgtcctgaa
     1021 gcgttttctc caggcgctgg atctccttgg cccgggcttc gacctgggtc tttcgatagc
     1081 gcgtgacgtc gcgaagttcc cgaatctcgg gcggcggcac aaaactcggc cgcaccatcc
     1141 cgtgggcggc gacatcggcc agccactcgg cgtcagagag gtcggtcttg cgccccggca
     1201 cgttcttcac gtgctgtgcg ttgcagagcc acagctcgtc gaacaaccct tcgaggcagt
     1261 aatagaccgg tttccaatag acgccggtcg cctccatcgc cacggtgtcg acgccggtgt
     1321 ctctcagcca tgcggccagc tcgccgagac ccttggcggt ggtggcgaag gactgcttcg
     1381 tcacctcgac ggaccgatcg ggttgtctga gctgacagca ggccacgacg gtgtctcgat
     1441 gcacgtccag gccggcgacc cgttcacgta ttcgtttcac tgcttcacct cccaggtatg
     1501 gatggaagg
//
LOCUS       DATAQL010000491         3635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5240090, whole genome shotgun sequence.
ACCESSION   DATAQL010000491 DATAQL010000000
VERSION     DATAQL010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5240090"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VHS97_08455"
     CDS             complement(<1..260)
                     /locus_tag="VHS97_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448274.1"
                     /translation="MVLRNLFGSFAWLAMAMAPSIVAADDQRIAEITSLGGRCLRNTD
                     GVVIGVDLSNTWVTDGDLEKIARLTHLESINLAYTKITDSGL"
     gene            complement(314..2344)
                     /locus_tag="VHS97_08460"
     CDS             complement(314..2344)
                     /locus_tag="VHS97_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448275.1"
                     /translation="MNTLARTIFVRAATLSALILLCFSITARTLHADPQDRQVAEWVL
                     LMRGSVRLEGRDERIREVTKLPPVDFRLDLVDLVGTNILPPDLKWLTGLKYLKTLNLP
                     GPMWNPGASARIDYSRDLSHIAGISSLEELIFSDTYLDFIKFDDKGIDAIAALAPGLR
                     VLSLANTQVRGHRLSAFKNLEALDLVYCPVDDEGLNATQGMTRLRKLLVRDAVISDES
                     LQNLRTLTNLEQLDLGGTRISDAGIAHLSGLTKLKKLNLQGAALTDEGIRHLAGMTDL
                     EELNLYGTRVTNVGADVLCRLKHLITVDLRYTRVTRAGVERLRTSVPRCHITFLAPSI
                     RPSLPAGADKIVASEGDTAVVEWVRSMGGQAVVDNGGLVAVSLTATSVTDELLLNLKD
                     SKRLCKLELQSTEIGDPGVRHLAKLTRLSQLDLSGTTISDLGLAHLAGLTGLRELRLA
                     HTQITGTGLKHLRSLALETLHMSGSPINDQGLAQLSAIVSLRDLALAFTDVTDAGLAQ
                     LESLTALQRLDLAGTDVGDKGLTRLGRMTQLAALLLNYTRITDKGLPELGKLERLQEL
                     ELTRTRITDTSMPVVGRLTKLVNLNLDYTDVSDKGLVALRGLTTLKSLSLDSTGVTDD
                     SAQPLGEFRQLRKLNLYHTFFTEPGCKRVRAALPQCEVIFDPKSSDPKRRRS"
     gene            2537..3634
                     /locus_tag="VHS97_08465"
     CDS             2537..3634
                     /locus_tag="VHS97_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3448276.1"
                     /translation="MPRPRVNQSGSPLGVLSLWLTRSFPVGRLHSRLEPLLKWLTKSG
                     RKNAEAINREFLDWLSHRQEQNRPFFAFLNYYDAHTPYLPPEGAGFSFGVRPRTLDDY
                     LLLIERWTMIDKSMLTPHYRDLINDCYDSCLAYLDQRLGELFDALERRGVLDRTVVIV
                     TADHGEELGEHRLFDHGVSLYRPEIRVPLLFVLPSDLPYPISPDKSADMPSDGSRTVP
                     FSPRRATVVRDTVSLRDLPATIVDLLGLTAGAPFRGRSLARLWRDSSSGAANDVCPAD
                     GAISELTLPNPTNPSQDRSPAARGPLVSLAEEDYVYIRHERDGHEQLFNERDDPGELS
                     NLAKVESMRPRLERLRARLDQMRSNPRQAAR"
BASE COUNT          696 a         1086 c         1087 g          766 t
ORIGIN      
        1 tccaggcccg agtccgtgat tttcgtatag gcaaggttga tgctctccag gtgcgtcagc
       61 cgcgcgattt tctcgaggtc accatccgtg acccacgtgt tgctcagatc gacgccgatc
      121 accactccgt ctgtattgcg gaggcagcgg ccgcccaaag atgtgatctc cgcgatgcgt
      181 tggtcgtctg ccgctacgat cgacggcgcc attgccatcg cgagccatgc gaaggaaccg
      241 aacaagtttc tcagtaccat ggtcgcacct gcccgtctag gatgttgtcg ctcgagcgct
      301 ccctacgagc tcatcagctt cgtcggcgct tgggatcgct cgatttcgga tcgaagatga
      361 cctcacactg gggcaaggcc gcgcggaccc gcttacaacc gggttccgtg aagaacgtgt
      421 ggtacaggtt cagtttgcgg agttgccgga attcccccag cggttgtgca gagtcgtcgg
      481 taacgcccgt gctgtccagg ctaaggctct tgagcgtcgt caagccgcgg agcgccacca
      541 agcccttgtc actgacgtcc gtgtagtcca ggttcagatt gacgagcttg gtcaagcggc
      601 cgacgacagg catgctcgtg tcggtgatcc gggtgcgggt cagctccagc tcctgaaggc
      661 gctcaagctt gccgagctct ggcagcccct tgtcggtaat tcgtgtgtag ttgagcaaga
      721 gggccgcgag ctgcgtcatt cttccgagcc tcgtgagccc tttgtccccg acgtcggtcc
      781 ccgccagatc gagccgttgg agcgcagtga gcgactccag ttgggcgagc ccggcatcgg
      841 tcacgtcggt aaaagccagg gccaaatccc gcagcgacac gatcgccgac aactgcgcta
      901 atccttgatc attgatgggt gaaccggaca tgtgaagcgt ctcaagcgcg aggcttcgca
      961 ggtgtttcag tccggtaccg gtaatctgcg tgtgcgcgag acggagctcg cgcaggcccg
     1021 tcaggccggc gagatgtgcc aggcctagat cggaaatcgt cgtcccgctc agatcgagct
     1081 gtgaaagcct cgtcaatttc gccagatgcc gaactcctgg atcgccgatc tcggtcgatt
     1141 gcagttccag cttgcatagg cgctttgaat ctttcaagtt gagcagcagc tcatcggtga
     1201 cactggtcgc ggtcagcgag acggcgacga gtccgccgtt gtcgacgacc gcctgaccac
     1261 ccatggaacg cacccactct accacggcgg tgtctccctc actggccacg atcttgtctg
     1321 ctccggccgg gagggagggc cggatggacg gggcgagaaa ggtgatgtga caccgcggca
     1381 ctgacgtccg gagacgttcg acaccggccc gcgtcacccg ggtgtaacgc agatcgacgg
     1441 tgatcaagtg cttgaggcgg cagagcacgt ccgcgccgac gttggtgacc ctggtgccgt
     1501 acaggttcag ctcttccagg tctgtcatgc ccgccaggtg ccgaattccc tcgtcagtga
     1561 gcgcggcgcc ttgcaagttc agctttttca gcttggtcag tccacttaag tgagcaattc
     1621 cggcatccga gatccttgtc ccgccgagat caagctgttc gaggttggtg agcgtgcgga
     1681 gattttgcag gctctcgtcg ctgatcacag catcccgtac caggagttta cgcagccggg
     1741 tcataccttg cgtcgcattg aggccttcgt cgtctacggg acaataaacc agatcgagtg
     1801 cctcaagatt tttgaacgcg cttaggcgat ggccgcgcac ctgggtgttt gccaagctca
     1861 acacccgaag gccaggagcg agcgcagcga tcgcgtcgat gcccttgtca tcaaatttga
     1921 taaaatcgag ataggtatca ctaaaaataa gttcttcgag gctggagatg cctgcgatgt
     1981 ggctcaggtc gcggctgtag tcgatgcgtg cgcttgcacc tgggttccac atggggccgg
     2041 ggaggttcag ggttttcagg tatttcagac cggtcagcca tttcaagtcg ggtggtagaa
     2101 tgttggtccc gaccaggtcc accagatcca gacgaaagtc aaccggaggc agtttcgtca
     2161 cctcgcgtat gcgttcatcc cgtccttcga ggcgtaccga accgcgcatc agcagcaccc
     2221 attcggccac ctgccggtcc tgtggatctg cgtgaagggt tcgcgctgta atggagaagc
     2281 agagcaagat cagagcggac agggtcgccg cacgaacgaa gatggtacga gccagtgtgt
     2341 tcatgactga tcgcataatg agaacttgat gattgcagcg taacgccgtg ttccttgacc
     2401 aaagcgtaca ctgccagaag ctcgggccgg cgggtgcaag cggccgtcta catgggggca
     2461 ttccgactat agcgaatgca ccgcatctgt gcaaagctgg ccctcgcgcc cgggaaggaa
     2521 ttgatttcgc cgaattatgc cccgaccgcg agtcaaccaa tcgggctccc cccttggcgt
     2581 cctcagtttg tggctaacgc ggagctttcc cgtaggacga ctgcactcac ggctggaacc
     2641 cctgctcaaa tggctcacga aatctggtcg aaagaatgcc gaagcgatca atcgggagtt
     2701 tctcgactgg ttgtcccacc ggcaagagca aaatcgcccc ttcttcgcct tcctcaatta
     2761 ctacgatgcg cacacaccct acttacctcc ggagggagct gggttctctt tcggcgtgcg
     2821 gccccgaact ctggacgatt atctgctatt gatcgaacgt tggaccatga tcgacaaatc
     2881 gatgttgacc ccgcattatc gtgacctgat caacgattgt tacgatagct gtctcgccta
     2941 cctggaccag cggctgggcg agctgttcga tgccctagag cggcgtggcg tgctcgatcg
     3001 gaccgtggtg atcgtgaccg ccgaccatgg tgaagagctg ggcgagcacc gcctgttcga
     3061 tcatggtgta agcctttatc gtccagaaat ccgcgttccg ctcctctttg tcttgccatc
     3121 ggacctcccg tacccgataa gtcctgataa gagcgcggac atgccttccg acggctcgcg
     3181 gactgtgccc ttctcgccaa ggcgcgcaac ggtggtgcgg gatacggtga gcctccgcga
     3241 tctgcccgcg accatcgtcg atctgctcgg tttgacggcc ggggcgccgt tccgcggccg
     3301 gtcactggcc cggctatggc gcgattcgtc ctcgggagcc gccaacgatg tttgccctgc
     3361 tgatggagcg atctccgagc tgacgcttcc caatccgacg aatccgagtc aggatcgatc
     3421 acccgctgct cggggcccat tggtctccct cgccgaagag gactatgttt acatccgaca
     3481 tgaaagggat ggacacgagc aattgtttaa cgagcgcgac gatccgggcg agttatccaa
     3541 tctggccaag gtcgaatcga tgcgtccccg actggagcgg ctgcgcgcac gtctcgacca
     3601 gatgaggtca aatccccgtc aagccgcccg ctgaa
//
LOCUS       DATAQL010000492         5130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2949947, whole genome shotgun sequence.
ACCESSION   DATAQL010000492 DATAQL010000000
VERSION     DATAQL010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2949947"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..1876
                     /locus_tag="VHS97_08470"
     CDS             383..1876
                     /locus_tag="VHS97_08470"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3448277.1"
                     /translation="MWIRQCDLDAESDFLPPIPARIASNEEFVPPPQSAQQKEYEDRL
                     ARISEETARRQGRSRRDFLRSGSGMAAALLALNQVFGDCYQVTADEVEDPQAFEEKWP
                     KDQFVFDVQTHHVDVSHKWYDETPTGRGIRNFFLMLRPDAKTMDQAVELLNRAHYVKE
                     VFGDSDTVMSIISGVPSRDWDKNPLPPDQMVETRRFVNDLAGSRRVLSHGLLRPNLGK
                     PELEEMERQVKELKIDAWKMYTGAEIGEKAWRMDDEKVAYPFWEKTKALGIKNLCVHK
                     GLPLGAFNEEACRPLDLEKAALDWPDLNFIVYHSGFRGFGWLASGTGIKVVDPKSTDP
                     QEIPWISDILRILKRNPKIKNIYFELGSTFGILSAAAPRTCMHMLGQMVQVAGADHIV
                     WGTDSIWNGSPQSQIERMRRIMIADDLIDIHKYPQLTDEVKDQILGLNAARLLGVDPA
                     AARKAIKGDKLSALREGRRYDPTTRSNTQYGWLWVEDGGVPTMPVGA"
     gene            1873..2166
                     /locus_tag="VHS97_08475"
     CDS             1873..2166
                     /locus_tag="VHS97_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448278.1"
                     /translation="MKFDTRVKRLIKAAILSVLAVFGCLALYFALLRHPGLFFRHTFI
                     ERSITLYSDEPIPSGSAVGVLERVEQRLARSPLFHPRSGDDIRIYICNRSWRF"
     gene            2454..3764
                     /locus_tag="VHS97_08480"
     CDS             2454..3764
                     /locus_tag="VHS97_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3448279.1"
                     /translation="MDSTTSEINDLIDQIKKAFRADDAARVRALLDDRPELKARINEA
                     LGPFDSPAIVNARSRPMLDVLIDAGADLNAKSRWWAGGFGLLHQCSPELAAYAIERGA
                     VVDVHAAARLGMIDRLRELIAQDPTLVHARGGDGQTPLHFASTVEVAEFLLDHGAEID
                     ALDVDHESTPAQYTVDHRQQVTRFLITRGCKTDILMAAALGDLKLVRTHLDADPECIR
                     MRVSEEYFPMVRPKSGGTIYQWTLGFHVSAHQVARKFGHEDVFQFLFDHSPEAIKLIV
                     GCWLGDEALVHTLRTQYPNIADSLTDDDRRQVARAARNNETTAVRLLLESGLPPDPGI
                     QHQATPLHWAAFHGNVTMAKLLLARNPPLEAEDADFHSTPLGWAIHGSENGWHCRTGD
                     YAATAEALIVAGAKVPEKIEGTEAVREVLRRHRPNESNSQHSPA"
     gene            3800..4036
                     /locus_tag="VHS97_08485"
     CDS             3800..4036
                     /locus_tag="VHS97_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448280.1"
                     /translation="MLDLLAALNKGDSAAGLIFSDLKKWIGYVGDFSEFLYGSDWPLA
                     PMVIYRRLIEAVIPKDHHQAVFRTNAEHVFGLGE"
     gene            4043..4963
                     /locus_tag="VHS97_08490"
     CDS             4043..4963
                     /locus_tag="VHS97_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3448281.1"
                     /translation="MHRLSTTVLLALRNITLHKFRVLLNLLGLIFGVSSVISTMAITE
                     GARLYTQRQFVARGATNIIIRSTQDGDDVGLSQHQTNEGDTLNFGLNYADFRRIMSTI
                     PTVVSATPLREDRNTESNSSDERIELSQVTVTIDTMEHVPRAAESLDRMLRQFHPNHD
                     YGITVPVELLQQAQATERNYDVFLGSIVSISLLAGGIGIMNIMLATVSARTHEIGIRR
                     ALGARRRDIIEQFLIETTVISSSGGLIGVLVGVAVPPLYSQLTGIPAVIRPWSPIIAF
                     LIAVSIGVIFGIYPAQRAAMLDPVEALRND"
BASE COUNT         1075 a         1597 c         1466 g          992 t
ORIGIN      
        1 atctcgaacg cttcgttgtc gcatccttcg tggcgcgccg tcacgccaac cggcgacaat
       61 gaaggaaact gtgccgcgac gagagatgtg accgtttcat ttgtaagtga ccgatcggcg
      121 gaccatccgt acgaatccat cttgatttcc accgtaaacg taaggaagta aaccataccc
      181 gttattactc gcacgagcgc cggacgaatc ggcacatcgg cctgtgcatc gtatcgctga
      241 tgtcgatcga ctgatccgta cgcccgtcat ccagactatt gtcagttcgc cacgggcggc
      301 ttattgttga gctcatgctc gattccacgg atcagccgcg gggaatcggg actctctccc
      361 aattcgacca ggagcgtcat ttatgtggat ccgccaatgc gatcttgatg ccgagtccga
      421 ctttctgccg ccgatcccgg cgcggatcgc ttccaacgaa gagtttgtcc cgcccccgca
      481 gtcggcccag caaaaggaat acgaagaccg gctggcccgc atcagcgaag agaccgcccg
      541 tcggcagggg cggtcgcggc gcgacttcct ccgcagcggc agcggcatgg ccgccgcgct
      601 cctggcgctc aaccaggtct tcggcgactg ctatcaagtc accgccgacg aagtcgagga
      661 tccccaggcc ttcgaggaaa agtggcccaa ggatcagttt gtcttcgacg ttcaaaccca
      721 ccacgtcgac gtcagccata agtggtacga cgaaacgccc accggccgcg gcatccgtaa
      781 tttcttcttg atgctccgcc cggatgccaa aaccatggac caagccgtcg agctgctaaa
      841 ccgggcgcac tacgtcaagg aagtcttcgg tgatagcgac actgtcatgt cgattatcag
      901 cggcgtgccc agccgcgact gggacaaaaa cccgctgccc cccgaccaga tggtcgaaac
      961 ccggcggttc gtcaacgact tggccggctc gcgccgagtc ttgtctcacg ggctcttgcg
     1021 gcccaatctc ggcaagcccg agctcgagga gatggagcgc caggtgaagg agctgaagat
     1081 cgacgcctgg aagatgtaca ccggcgccga gatcggcgaa aaggcgtggc ggatggacga
     1141 cgagaaagtc gcctacccgt tctgggaaaa gaccaaggcc ctcggcatca aaaacctctg
     1201 cgttcacaaa ggattgccgc tgggggcatt caacgaggaa gcgtgccgcc cgctcgacct
     1261 cgaaaaggcc gccctcgact ggcctgacct gaattttatc gtctaccatt ccggcttccg
     1321 cggtttcggc tggctggcca gcggcaccgg catcaaggtc gtcgatccca aaagcaccga
     1381 cccccaggaa atcccatgga tcagcgacat cttgcgcatc ctcaaacgga accccaagat
     1441 caagaacatc tatttcgagc tcggcagtac ctttggcatc ctgtcggccg ccgcgccgcg
     1501 gacatgtatg cacatgctcg gccagatggt ccaggtcgcc ggcgctgacc acatcgtgtg
     1561 gggcaccgat tcgatctgga acggcagccc ccagagccag atcgagcgga tgcggcgaat
     1621 catgatcgcc gacgatctga tcgatataca caaatatcca caattgactg atgaagtaaa
     1681 ggaccagatc cttggtctca acgcggcgcg gctgcttggc gtcgatccgg cggcggcgcg
     1741 aaaggcgatc aaaggggata agctaagtgc cttgcgggag ggccggcggt acgatcccac
     1801 aactcgcagt aacacccagt acggctggct ctgggttgaa gacggcggcg tgccaacgat
     1861 gcccgtgggg gcgtgaaatt cgatacccgc gtgaaacggc tgatcaaagc ggctattctt
     1921 tcagtcctgg cagtattcgg ctgtcttgct ctttactttg ctcttctgcg acatccgggc
     1981 ctcttcttcc gccacacttt cattgagcgc agtatcacgc tctattcgga tgagccgatt
     2041 ccctctggtt ccgctgtcgg cgtgcttgag agggtggaac aacgtcttgc acggtccccg
     2101 ttgtttcatc ctcgttccgg cgatgacatc cggatctata tctgcaatcg gagctggcga
     2161 ttttgaccga tattcggatt ctatcgccaa gggagcgagc ttcgactacg acgacgcact
     2221 ggcccagctt cggcagggag atccgaaaat ggacccccgg cgctctggcc tctatttgcg
     2281 atacaatctc ttagtcgcct atctacttga tcgcaagaga atcagcgtcc atgagatgct
     2341 gagccaagat ttcgatcgag caagtctcga agccgaaatg ctggcggaga atcatggatc
     2401 gtgaccgcac tagcgccacg gatcgcaaat gacggccaca ccggagagaa cccatggact
     2461 ccacaacatc cgagataaac gatctgatcg atcagatcaa aaaagcgttc cgggccgatg
     2521 acgcggccag ggttcgcgcg cttctcgacg accgacctga actcaaggcc cgcatcaacg
     2581 aagctctggg cccgttcgat tcaccggcga tcgtgaacgc ccgcagccgc ccgatgcttg
     2641 atgtcttgat cgacgcgggc gccgacttga acgcaaagag tcgctggtgg gccggcggtt
     2701 ttgggctctt gcatcagtgc agtcccgagc tggccgctta cgcgatcgag cgcggtgccg
     2761 tggtcgacgt ccatgccgcg gcacgtctgg gcatgatcga tagacttcgt gaactgatcg
     2821 cgcaggatcc gaccctcgtg cacgcacgcg gcggggacgg tcagacaccc cttcactttg
     2881 ccagtaccgt tgaagttgca gagttcttgc tcgaccatgg cgcggaaatc gatgcgctcg
     2941 acgtggatca cgaatcgacg ccggcacagt acacagtgga ccatcgccag caagtgacgc
     3001 ggttcttgat cacgcgcggc tgcaagaccg atatcctcat ggccgccgca ctgggagatc
     3061 tcaagcttgt ccgtacccat ctcgatgcgg atccggaatg cattcggatg cgggtgagcg
     3121 aagagtactt ccccatggtc cgcccgaaaa gtggaggcac gatctaccag tggactctgg
     3181 gcttccatgt gtcggcgcat caggtcgcac gcaagttcgg ccatgaagac gtattccaat
     3241 ttctgttcga ccacagccct gaggcgataa agcttatcgt gggctgctgg ctgggtgacg
     3301 aagcgttggt gcacaccttg cggacgcaat atccgaacat cgcagacagt ctgaccgatg
     3361 acgaccggcg ccaggttgca cgtgccgccc gcaacaatga gacgaccgcg gtccgtctgc
     3421 tgctcgaaag cggtctgccc ccggacccgg gcatccagca ccaggcgaca ccgctccact
     3481 gggccgcgtt tcacggcaac gtcaccatgg ccaagctgct gctggcccgc aacccgccac
     3541 tggaagccga ggacgccgat ttccactcga ccccgctggg gtgggccatc cacggctcgg
     3601 aaaacggctg gcattgccgg acgggagact atgccgcaac ggccgaagcg ctgattgttg
     3661 cgggggcaaa ggtacccgag aagatcgagg gaaccgaagc cgtccgcgag gtgctgcgcc
     3721 ggcacaggcc aaacgaatca aactcacaac actcgccggc atgaatgcgg tagatcgacc
     3781 gctttgcaga aaaaaggaga tgctggatct tttggccgct cttaacaaag gcgactcggc
     3841 cgccggcctg atcttctcgg acttgaagaa gtggattggc tacgtcggcg actttagcga
     3901 gtttctctac ggcagcgact ggccgctggc cccgatggtt atttatcgcc gtttgatcga
     3961 ggccgtcatt cccaaggacc atcaccaggc tgttttccga accaatgccg aacatgtgtt
     4021 tggccttggg gaataacccc tcatgcaccg cctctccacc acggtcctcc tcgcgctgcg
     4081 caacatcaca ctgcacaagt tccgtgttct cttgaatctt ctgggtctga tcttcggcgt
     4141 ctcgtccgtc atctccacga tggcgatcac cgaaggtgcg aggctgtata cccagcgcca
     4201 gttcgtcgca cggggagcca ccaacatcat catccgcagc acgcaggacg gcgatgacgt
     4261 cggcctctcc cagcaccaga caaacgaggg cgacaccctc aatttcggcc tgaactatgc
     4321 ggatttccgt cgcatcatgt ccaccattcc caccgtcgtg agcgcgacac cgctgcgcga
     4381 ggaccgcaac acggaaagca actcgtccga cgagcggatc gagctgtccc aggtcaccgt
     4441 cacgatcgat acgatggagc atgtgccgcg cgccgccgaa tcacttgatc gcatgcttcg
     4501 gcaatttcac ccgaatcacg attacggcat cacggtcccc gtcgagctac tgcagcaagc
     4561 ccaggccaca gagcgcaact atgatgtatt cctgggctcg atcgtcagca tctcgcttct
     4621 ggccggcggt atcgggatca tgaacatcat gttggcgacg gtctcggcgc ggacccacga
     4681 gatcggcatt cgccgcgcgt tgggagcgcg ccgacgcgac atcatcgagc agttcctgat
     4741 cgagacgaca gtcataagct cgtccggcgg tctgatcggc gtcctggtgg gcgtggccgt
     4801 cccaccgctc tattcccagc tcacgggcat ccccgccgtg attcgccctt ggtcgccgat
     4861 catcgccttt ctgatcgcgg tctcgatcgg ggttattttc ggcatttacc cggcccaacg
     4921 ggccgcaatg ctcgacccgg tcgaagcact ccgcaatgac tgacagcccg caggtatcga
     4981 tcgcccagaa aaccggcggg gaatcacttg gcgatttcat cacttggtag cgtgtaaagt
     5041 ctcgagaagc atccacggaa attcgagacg gattgaaaaa cggcctcccg tcgacgatga
     5101 ggttcgtttc gtcggcaatg attcggaagg
//
LOCUS       DATAQL010000493         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_49378, whole genome shotgun sequence.
ACCESSION   DATAQL010000493 DATAQL010000000
VERSION     DATAQL010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49378"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VHS97_08495"
     CDS             complement(<1..954)
                     /locus_tag="VHS97_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HEX3448282.1"
                     /translation="MRVQRADRSRIAGISLACLALVCSQKAQAQVKLEYKFTEGNKLT
                     YKTTIKTKQVLTLQGMEIETESDQVAVSSRTVGKRRADSSTPIEDKVESLRVELSLPG
                     GMNVTVDSTNPDVKIDNPALAFLGEILKLAGETNYTVVLDNRNKVQAIEGIEKLLEKA
                     DKLSPQARDAIRDRLNADKLKARFEEEHGNIPDVLCRPGEPWERTEVLDVGSGQTLVF
                     KKRYEYVGTEKKGDKTLDKISSKCTGAELKQDPDANSPLKVVKGDLKVDDSGGILLFD
                     RENGRVFSATAKTRLKGDMMTFSYEGNELPGALDLTIESNVE"
     gene            1297..>1645
                     /locus_tag="VHS97_08500"
     CDS             1297..>1645
                     /locus_tag="VHS97_08500"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3448283.1"
                     /translation="MAPLISESHHTSIAPVTDTSSVSRGYNRWAAVYDHDLNPLPALE
                     EPVFRAAVGDVRGLSVVDLGCGTGRHAIWLAGADALVTAIDFSEGMLTEARAKPGADR
                     VRFLTHDLHQPLPL"
BASE COUNT          307 a          493 c          459 g          386 t
ORIGIN      
        1 ttccacgttg gactcgatgg tcaggtccag tgcgcccggg agttcgttac cttcgtatga
       61 gaaagtcatc atgtccccct tgagcctggt ctttgccgtg gcgctaaaca cgcgaccatt
      121 ttcgcgatcg aagagcagga ttccccctga atcgtcgacc ttcagatcac ctttgaccac
      181 cttgagcggc gaattggcgt cgggatcttg cttgagctcg gctcctgtgc atttagagct
      241 gatcttgtcg agcgttttgt ctcccttttt ttcggtaccg acatactcgt accgcttctt
      301 gaaaacgagt gtctggccgc tgccaacgtc gagaacctcg gtccgttccc agggttcgcc
      361 cgggcggcac aggacgtccg gtatgttgcc gtgttcctcc tcgaaacgcg ccttgagctt
      421 gtcggcgttc aggcgatcgc ggatcgcgtc ccgagcctga gggctgagct tatcggcctt
      481 ttccagtagc ttttcgatcc cttcgatcgc ctggaccttg ttccggttat cgagcacgac
      541 cgtgtagttg gtttctccgg ccaacttgag tatttcgccc aggaaagcca gggcaggatt
      601 gtcgatcttg acgtcgggat tggtcgagtc gacggtcacg ttcatacctc ccgggagcga
      661 taactcgacg cgcagcgatt cgactttgtc ctcgatcggt gtgctggaat cggcacgccg
      721 cttgccgact gtccgcgacg agacggccac ctggtcgctt tccgtttcaa tttccatgcc
      781 ctggagcgtc aagacctgct tggtcttgat cgtcgtcttg taggtgagct tgttcccctc
      841 ggtaaacttg tactcgagct tgacctgagc ctgggctttc tgtgaacaaa cgagcgcaag
      901 gcacgccaag ctgatcccgg cgattcttga gcgatccgca cgttgaactc tcacgaatag
      961 tctcccccgc ccatggcaaa agaatgactg aaagccgtgt gaacgcagat ccacctgacg
     1021 atctctgcga gtattcgccc gagccaccag catatttgac cggcatcaaa gactgaagac
     1081 caatgcggta cgcttgtcgc tcgacggccc tggggaggac acaaatgccg gccctcgcgg
     1141 cgataatcga ccgatctcga ccgagaccat cgctggagct acactcgtcc ggaggaaacg
     1201 acagccgaat cttagcctgc gcgggccaga cacccgtcgg cacgatgatc ttagaaaaac
     1261 catcgtcgtc gatcgcgctg acacaggaat gatcgaatgg cgcctctaat ctccgagtca
     1321 catcatacat caatcgcacc cgtcaccgac acttcctctg taagtcgcgg ttataaccgt
     1381 tgggcggcgg tttacgatca cgacttaaat cccctcccgg ctctggaaga gcccgtgttc
     1441 cgcgcggcgg tgggagatgt tcgcggcttg tcggtcgtcg acctcggctg cggcacaggc
     1501 cggcacgcga tctggttggc aggcgctgat gcgttggtta cggcgattga tttttccgaa
     1561 gggatgctga cggaggcgcg cgccaaaccg ggtgccgacc gggtcaggtt tctgactcac
     1621 gatctgcatc aacctctacc attgc
//
LOCUS       DATAQL010000494         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4781159, whole genome shotgun sequence.
ACCESSION   DATAQL010000494 DATAQL010000000
VERSION     DATAQL010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781159"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1949
                     /locus_tag="VHS97_08505"
     CDS             54..1949
                     /locus_tag="VHS97_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEX3448284.1"
                     /translation="MRFSWRTLVFLLGSACLANAADDTLVSSALDRDPQGWTDLLAKA
                     GSKLEGWTRGPLPAEGKLRPGSQWSLDAATGSLVCQGDGGHEWLRWDNELGDFIYHVE
                     WRFTPVPGKKGYNSGVYARNSGDGRIYHQAQTGDGSGGYLFGVTQTNGSPARFNLSKQ
                     HPDSRVKPAGEWNTFEITCQGKDMTLWVNGAVVNQWHECEVKRGYVGLEAEGWRIEFR
                     NVKVKPLAKLQVGAAVRVITPDPLLPVSGGMGTPNPAREKRGEITARAIVLRKEDISV
                     AIVSLDLLGFPSVLGDRVRAKVSRISADHILIGSTHTHSAPDCYAFPDGKGGHTGDLQ
                     YLALVCEKAAEAVNEAIDRLQPASIKVATGEAQGKIAYNYYAPDLYDRRMSVIQAVSL
                     QGKPIATLVNYAVHPEVLGNDIGVLSPDLIGPLCEKIESKGGGTALFMNSAQGGMVTA
                     DNRDLNQPRDQQRGYWNDSRSWDECLRIGHLMAAEAARIIKDAPEQLDPSLYCDSINV
                     RFPVDSTLMWAVIKGSPLKYPHNDDQSISARINLLNLGNAQILTIPGEALPNIGFYLK
                     RKMHGKNNMLFGLTNDAFGYILTKVDFQSFPRYDYVSRTSLGEMTGEILIEKSLELVN
                     KSPVSDR"
     gene            2151..>2966
                     /locus_tag="VHS97_08510"
     CDS             2151..>2966
                     /locus_tag="VHS97_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX3448285.1"
                     /translation="MGFDANFRRLGLAAAVLAFWAGGQPRARGDVKVPAIFGSQMVLQ
                     RDQKDRVWGWAEPGEDVTVKIAGQSHSTKAGGDGSWQVELDPMPAGGPHTLAIEGKNK
                     LEFDQVLVGEVWICSGQSNMQWSVAAAKDADLEIATAKYPLIRLISVPNVGTQEPQKD
                     FRGRWQPCQPDTVGRFSAVGYFFGRRLHQVLDIPVGLINDAWGGSACEAWIRRDILAA
                     DEKYGPLLKRWDRLEKNLPQAKAEYESRLAEWTKAAAKARADGKEAPRRPVNPE"
BASE COUNT          604 a          870 c          920 g          572 t
ORIGIN      
        1 tgatggcagc cggtttggtc cattggcccc ttaatcagcc cgggagttgc ccgatgcgtt
       61 tctcgtggag aactctggtt ttcttgctcg gatcggcctg cttggctaac gcggccgatg
      121 acacgctggt ctccagcgcg ctcgatcgtg atccccaagg ttggaccgac ttgctggcca
      181 aggccggttc caaactcgag ggctggaccc gcgggccgtt gccggccgag ggcaagcttc
      241 ggcccggctc gcagtggtcg ctggacgcgg cgactgggtc gttggtatgc cagggggacg
      301 gcggccatga gtggctccgc tgggacaacg agctgggcga cttcatctac catgtcgagt
      361 ggcggttcac gccggttccg ggcaagaaag gttataactc gggcgtctac gcccgaaact
      421 cgggcgatgg ccggatctat caccaagccc agacgggcga cggctcaggc ggatatttgt
      481 tcggcgtcac ccagacaaac gggtcgcccg cgcggttcaa tctttccaag cagcatccgg
      541 attcacgcgt caagccggcg ggcgagtgga atacctttga aatcacctgc caggggaagg
      601 acatgaccct gtgggtcaac ggagccgtcg tcaatcagtg gcatgagtgc gaggttaagc
      661 ggggctacgt cgggctcgag gccgagggat ggcggataga attccgcaac gtcaaggtca
      721 agccgctcgc gaaacttcag gtcggcgcgg cggtgcgcgt gatcacaccg gatcccctat
      781 tgccggtatc gggcggaatg ggcacgccga atcccgcgcg ggaaaaacgg ggtgaaatca
      841 cggcccgagc gatcgtcctt cgaaaagaag acatctcagt cgccatcgtg tcgctcgact
      901 tgctcggttt tccgtcggtg ctgggggatc gtgtgcgtgc aaaagtgagt cgcatttcgg
      961 ccgatcatat tttaatcgga tcgacgcata cccacagcgc ccccgattgt tacgcttttc
     1021 ccgacggcaa gggggggcac acaggcgacc tccaatacct ggcattggtt tgcgaaaagg
     1081 cagccgaagc ggtcaacgag gccatcgacc gcctccagcc agcctcgatc aaggtcgcca
     1141 cgggcgaggc gcagggcaaa atcgcgtata actactacgc ccccgatctc tacgaccgcc
     1201 ggatgagcgt catccaggcg gtcagcttac aaggcaagcc gatcgccacg ctcgtcaatt
     1261 acgcggtcca cccggaagtg ctcggcaatg acatcggggt tctcagcccc gacctgatcg
     1321 gtcctctttg cgagaagatt gagtcaaagg gcggcggcac ggccctgttc atgaatagcg
     1381 cccagggggg catggtcact gccgacaacc gcgacttgaa ccaaccacgc gaccagcaac
     1441 gaggctactg gaacgattcg cggtcgtggg atgaatgcct gcgaatcggc cacttgatgg
     1501 ccgctgaagc ggcccgcatc atcaaagacg ccccggagca gttagacccg tcactttatt
     1561 gcgactccat aaacgtgcga tttccagtgg attcgacgct gatgtgggcc gttatcaaag
     1621 gttcaccgct caagtatccc cataacgacg accagtcgat cagcgcgcgc atcaacctgc
     1681 tcaaccttgg caatgcccag attctgacga tcccgggcga agccttgccc aatattggct
     1741 tttatctcaa gcgcaagatg cacggcaaaa acaacatgct gtttggcctc acaaacgatg
     1801 cgttcggcta catcttgacg aaggtcgatt tccagagctt tccccgctac gattatgtct
     1861 cgcgcacgtc gctcggcgag atgactggtg agatcctgat cgagaaatct ctcgaattgg
     1921 tcaacaaatc gccggtctcc gatcgctaag gtgtcgggtc tctggatcgc ttgggtcgag
     1981 ttttgctggc ccagtacttg ctctccgccg gacacaaacc cctcatgctg ccaacagatt
     2041 gctcggcgag ggtcttcttt gagagctttc tctctggcat gatgagtctc tgcacgccag
     2101 agggtctgat gaattcggag atacattgac tttgaagtga ggatcaaccg atggggttcg
     2161 atgcgaactt tcggcgattg ggcctggccg cggcggtact cgcgttctgg gccggggggc
     2221 aacctcgggc ccgcggcgat gtgaaggtgc cggcgatctt cgggtcgcag atggtattgc
     2281 agcgcgatca aaaagaccgg gtctggggtt gggccgagcc tggtgaagac gtcactgtca
     2341 agatcgccgg ccaaagccat tcgaccaagg ccggcggtga tggctcatgg caggttgagc
     2401 tcgacccgat gccggccggc ggtcctcaca ccctggccat cgaggggaaa aacaagctcg
     2461 aattcgatca agtgctcgtg ggcgaggtct ggatctgctc aggtcagtcc aatatgcagt
     2521 ggtcggtggc cgcggccaag gacgcggatc ttgaaatcgc aaccgccaag tatcccttga
     2581 ttcgactgat cagcgtcccc aacgtcggca ctcaggagcc ccagaaagac ttccgcggcc
     2641 ggtggcagcc atgccaacct gatacggtcg gccggttctc tgcagtgggc tactttttcg
     2701 ggcgacgact gcaccaggtg ctcgacattc ccgttggctt gatcaacgac gcatggggtg
     2761 gatcggcgtg cgaggcctgg attcgccgcg acatcttggc cgcggatgag aaatatggcc
     2821 ccctgctgaa gcgctgggac cggctcgaga aaaacctgcc ccaagccaag gccgagtacg
     2881 aatcaagact ggccgaatgg acgaaagccg ccgccaaagc cagggccgat ggcaaggagg
     2941 cgccccgtcg tcccgtgaac ccagag
//
LOCUS       DATAQL010000495         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3102028, whole genome shotgun sequence.
ACCESSION   DATAQL010000495 DATAQL010000000
VERSION     DATAQL010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3102028"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..1786)
                     /locus_tag="VHS97_08515"
     CDS             complement(431..1786)
                     /locus_tag="VHS97_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX3448286.1"
                     /translation="MQPNRRDALRFVAGGILGCTFGARCRALTVDSAQPPLTIAGLKV
                     TPIALPDPPIMAASGCHGPYFLRNVVELKVEGGIVGIAEVPGGERVTRALQQAEKLVV
                     GQNAFAYRKFAHELQALGMGCYAGIELACLDASARATGRRLCELVGGPVRESVEFAAY
                     LFYRYAADHPVVLSDPHLVDSRGRGDHALDSWGEVRSAETMAAMAAQFRDRWGFRVFK
                     LKGGVLDPEIERDSLKAMAAKLGREALLRIDPNGRWKTATAIRIGKEIANLPLEYYED
                     PVQGQAAMAEVRRATGLKMSTNMCVTSFGHIPEAFKLKPIDVLLCDHHYFGGFAGCQA
                     LGPVCTAAGWSMSQHSNSHAGVTMAAMIHLAASIPELTMASDTHYPWLIETADIIEGP
                     KLIIRDGKMNVPAGPGLGVTLDQDKLARAHETYTKCGMRGRDDRTLMRRLEPGWTGAL
                     L"
     gene            complement(1825..2127)
                     /locus_tag="VHS97_08520"
     CDS             complement(1825..2127)
                     /locus_tag="VHS97_08520"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX3448287.1"
                     /translation="MESIIAQHITKTPGVCGGTACIAGHRIRVMDIVVLHEMRGMCPE
                     EVAYQYPTITLADVHAALAYYFDNVEEIQNEFRKDEEWAEWAKTNVPSKLREKLRG"
     gene            complement(2270..2770)
                     /gene="smpB"
                     /locus_tag="VHS97_08525"
     CDS             complement(2270..2770)
                     /gene="smpB"
                     /locus_tag="VHS97_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX3448288.1"
                     /translation="MSKKDKGKAKAKEKTDDSGIKIVARNRRARHDYELLEKVEAGLV
                     LTGTEVKSLRNGKANLEDAYAEVNDGEAWLVGCDIPEYVQANRMNHVPKRPRKLLLHR
                     REISKLETKTGEKGMTLVPLSIYFKKGIAKVELSVAKGRKTFDKREAIKKQDAKREID
                     RALRRR"
     gene            complement(2878..>3136)
                     /gene="folK"
                     /locus_tag="VHS97_08530"
     CDS             complement(2878..>3136)
                     /gene="folK"
                     /locus_tag="VHS97_08530"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX3448289.1"
                     /translation="RILQDIEGQFGRVRSVRWGERTLDLDVLLIGDEIISTRELTVTH
                     PRLRVRRFVLEPLADVAPEAVDPVTKRTIADLLAALEEGPS"
BASE COUNT          612 a          973 c          872 g          679 t
ORIGIN      
        1 tgaccaacct cgacacgcca ctagtcgctt cagacatccg gtcgatcctg gttcgccccc
       61 caaattgtca aatacttcgc tcaactgagc cctgaatggg accctttaat tattcgtcgt
      121 accaacatcg ggtcaagcac gattatcgac aacgccacga aaccgcatgc gagagtgcgc
      181 ttgctatttc ctgatgccgc tcaatcccaa agaacgaacg tgctgatatt gaataagcgg
      241 tgagagcgtt gatcggatgt acatgcggtc accagcacgc tcgcgcgggg gaattctcat
      301 gagccagcct tctacgttac acgcaggttc ctttgcacgg gacagtccat ttactcccac
      361 aagtcatgat acacgcattg aggtcagaag ggtcgtactg gcatccgtgg gcgaacgccg
      421 tgttcgactt tcagagaagc gcccccgtcc aaccaggctc gagtcttcgc atcagggttc
      481 ggtcgtcgcg tccccgcatc ccgcacttcg tataagtctc atgagcacgc gccagcttgt
      541 cttgatcgag cgtcaccccg agaccaggac ccgccggaac gttcattttt ccatcccgaa
      601 taatgagctt tggtccctcg atgatgtccg cggtctcgat tagccagggg taatgagtgt
      661 cactggccat ggttagctca ggaattgagg cagccagatg aatcatcgcc gccatcgtca
      721 cgccggcgtg gctgttgctg tgctggctca tcgaccatcc agccgccgta cagaccgggc
      781 ccagcgcctg gcagccggcg aagcctccga aataatgatg atcgcacagc aagacgtcga
      841 tcggtttcag cttgaacgct tcgggaatgt ggccaaagct ggtcacgcac atgttcgtgc
      901 tcatcttcag gccggtcgcg cggcggactt cggccatggc cgcctgaccc tgcactgggt
      961 cttcataata ttccagcggc aggtttgcga tttccttgcc gatccgtatg gccgtcgccg
     1021 tcttccagcg tccgttgggg tcaattctca agagggcctc gcgacccagt tttgccgcca
     1081 ttgccttcag gctgtcgcgc tcgatttctg ggtcaaggac gccccccttg agtttgaaga
     1141 cccgaaagcc ccagcgatcg cggaattgcg ccgccatcgc ggccatggtc tcggccgagc
     1201 ggacctcacc ccacgagtcg agtgcatggt cgccgcgacc tcgcgaatcg acgaggtggg
     1261 gatcactcaa tacgaccgga tgatcggcag cgtagcggta aaaaagatac gccgcgaact
     1321 cgaccgactc gcgcaccggg ccgccaacca gttcacacaa tcggcggccg gtcgcccgcg
     1381 cgctggcgtc gagacacgcg agctctatgc cggcataaca gcccatcccc agcgcctgca
     1441 attcgtgtgc aaacttgcgg taggcgaagg cgttttggcc gacgacgagc ttttctgcct
     1501 gctggagggc tctcgtcaca cgttcgccgc cgggtacttc ggcgattcca acgatgccgc
     1561 cttcgacttt caactcgacc acattccgca aaaaatacgg gccatggcag ccgctggcgg
     1621 ccatgatcgg cgggtctggg agagcgatcg gggtcacttt caagccggca atggtcaacg
     1681 ggggctgcgc cgaatccacg gtaagtgccc gacatcgcgc gccgaatgta catcccagaa
     1741 tgccgccagc aacgaagcgc agcgcgtcgc gacgattcgg ttgcaacgtc cgagattcct
     1801 gaagcgggtc gctcataaat cggttcaacc acggagtttt tcgcgaagct tcgatggaac
     1861 gttggttttc gcccactcag cccactcttc gtctttccga aactcgttct ggatctcctc
     1921 cacgttgtcg aagtagtacg ccagcgccgc atgtacgtcg gccaaggtaa tggttgggta
     1981 ctgataggca acttcctcag gacacatgcc ccgcatctcg tgcagaacaa cgatgtccat
     2041 tacccgaatg cggtgcccgg cgatgcacgc cgtcccgcca cagacgcctg gagttttcgt
     2101 aatgtgttgc gcgataatgc tttccatcgg tgtcgccgga gccgacgctt gctggcgtcg
     2161 aacgatacgc gttcctcctt atctatcatc cgtttgtctg gcgcgatgga caagctgcgc
     2221 gaaccggggc gctcagtgca tgcgatgatc gccaaccgga tcaaaggtct catcgcctcc
     2281 gcaaagcgcg atcgatttcg cgtttggcat cctgcttctt gatcgcttcg cgcttgtcga
     2341 aggttttgcg gcccttggca acgctcaatt cgaccttggc aatccccttc ttgaagtaga
     2401 tggagagcgg tacgagagtc atcccctttt caccggtctt ggtttcgagc ttgctgatct
     2461 cgcggcggtg aagcaagagt ttccgtgggc gctttggaac atggttcatt cggttggcct
     2521 ggacgtactc ggggatgtcg caaccaacga gccaggcctc gccgtcattg acctcggcat
     2581 aggcatcttc caggtttgcc ttgccattcc gcagactctt gacctctgtc ccggtcaaca
     2641 cgaggcccgc ctcgaccttt tccaggagct cgtaatcgtg gcgcgcgcgg cggttacgcg
     2701 cgacgatctt gattccggaa tcgtcggtct tttctttggc ctttgccttg cctttgtctt
     2761 tcttggacat ggccaaccta actgtcacta gggccgtaaa cccaatggag cggcgtgtgt
     2821 gaacgaatca taaagggccg cctacccagc aacaagggtc cgctctggct gaattctcta
     2881 gctcggtccc tcttcgagag cggcaagcaa atcggcgatc gtacgcttgg tcaccggatc
     2941 gacggcctcg ggagcaacgt cagcgagcgg ctcaaggacg aaacgacgca cccgcagccg
     3001 cggatgggta acggtcagct cgcgcgtgct gatgatctcg tcaccgatca gcagcacgtc
     3061 aaggtccagc gtccgctcgc cccagcggac cgatcgaacg cggccgaact ggccttcaat
     3121 atcctgtaaa attctg
//
LOCUS       DATAQL010000496         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_201415, whole genome shotgun sequence.
ACCESSION   DATAQL010000496 DATAQL010000000
VERSION     DATAQL010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201415"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /gene="uvrA"
                     /locus_tag="VHS97_08535"
     CDS             complement(<1..1599)
                     /gene="uvrA"
                     /locus_tag="VHS97_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246145.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HEX3448290.1"
                     /translation="MPKPRGKKTATAPKAEKTKPPSSIQVRGARVHNLKNLDVDLPRD
                     SLVVLTGVSGSGKSSLAFDTIYAEGQRRYLECLSSYARQFLDQLERPDVDAIEGLPPT
                     VAIDQRRGTANPRSTLGTVTEIYDYLRLLFARVGKPHCPVCGVPIHRQTPEQMAAELM
                     RLREGQRVQILAPLVRDRKGQHLEVFQAIRRAGLIRARVDGEMIEVTDQPPKLAKTRS
                     HSIEAVVDRISVREGIRPRVAESLDLALKLSGGGVLTLTESAEGWDEHFHSIHLSCPN
                     CGTGLPTIEPRSFSFNSPHGACPVCQGLGSRSSFQPELVFTDHSRSWNQHAAVLWPLI
                     APAARDAPAYDSQIKAFLERHKIDGATPLQDWPAGLLDLFWSGEPNGTFPGLVAILEH
                     SFEDCRDDASRKSLLVYREDVLCAACGGSRLRQEARAVQLGGRSIADLTALAVLDLLT
                     FFEQLPIEPSLGAVGSPLVAEVVGRLQYLIEVGLNYLSLGRGSDTLSGGELQRARLAA
                     QLGSGLTGVCTILDEPTTGLHPRDT"
BASE COUNT          326 a          512 c          502 g          308 t
ORIGIN      
        1 ggtgtcgcgg ggatgaaggc cggtcgtcgg ttcgtccagg atcgtgcaca cgccggtcag
       61 gcccgagccg agctgcgccg ccagtcgagc ccgctgcagc tctccgccgg agagcgtgtc
      121 ggatccgcga ccaagtgaca gataattgag gccaacctcg atgagatact gcagtcttcc
      181 cacgacctcg gcaaccaacg gcgatccgac ggcgcccagc gacggttcga ttggtaattg
      241 ctcgaagaat gtcaacaggt caagtaccgc aagcgccgtc aggtcggcaa tcgatcggcc
      301 gcctaactgc acggcacgcg cttcctgtcg aaggcgggat cctccgcagg ccgcacagag
      361 aacgtcctcc cggtacacaa gaagtgattt tcgactcgca tcgtcacgac agtcctcaaa
      421 cgaatgctca agaatcgcga ccagtcctgg gaaagttcca ttcggctcgc ccgaccagaa
      481 caggtcgagg agacccgccg gccagtcttg tagtggtgtg gcgccgtcga ttttgtggcg
      541 ttcgagaaaa gccttgatct gcgaatcata cgccggtgcg tctcgagccg ctggagcgat
      601 caatggccaa aggacggccg cgtgctgatt ccacgaacga gaatgatcgg tgaataccag
      661 ctcgggctga aatgaagagc gcgagcccag cccctgacag accgggcaag caccatgcgg
      721 actgttgaag ctaaaactcc ggggctcgat cgtcggcaag ccggtaccgc aattgggaca
      781 gctgagatgg atgctgtgaa aatgctcgtc ccagccctca gccgattcag tcaacgtcag
      841 gacaccgccg ccggagagct tgagcgccag gtcgagactt tccgctacgc gtggccggat
      901 tccttcgcgc accgagatcc ggtcgaccac ggcttcgatc gagtgcgacc gtgtcttcgc
      961 cagcttggga ggctggtcgg tcacctcgat catttcgccg tccacccgcg cacgaatcaa
     1021 gcccgcgcgg cggatcgctt gaaagacctc gaggtgctgt cccttgcggt ctcgtaccag
     1081 gggggccagt atctggaccc gctgtccctc tctgagcctc atcaattccg cagccatctg
     1141 ctcgggagtc tggcgatgga tcggcacgcc gcaaacaggg caatgaggct taccaaccct
     1201 ggcaaacaag aggcgcaagt aatcgtagat ctcggtcacg gtcccgagtg tactacgcgg
     1261 attagccgtc cctctgcgct ggtcgatcgc caccgtcggc ggcaagcctt caatcgcgtc
     1321 gacgtcaggc cgctcgagct ggtcgagaaa ctgccgtgcg tagctcgaga ggcattcgag
     1381 atagcgacgc tggccttccg cgtagatcgt atcgaacgcc agcgagctct tccccgatcc
     1441 gctgacaccg gtcaagacga ccaagctatc gcgcggcagg tcgacgtcga gattcttcag
     1501 attgtgcacg cgagcaccgc ggacctggat cgaggagggc ggcttggtct tctccgcctt
     1561 cggggcagtc gccgttttct tgccgcgggg ttttggcacg ggatcgcgtt tcctcattgg
     1621 ccggggtata cctttccgtt tgaatcaa
//
LOCUS       DATAQL010000497         5473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2645042, whole genome shotgun sequence.
ACCESSION   DATAQL010000497 DATAQL010000000
VERSION     DATAQL010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2645042"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..3041
                     /locus_tag="VHS97_08540"
     CDS             474..3041
                     /locus_tag="VHS97_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3448291.1"
                     /translation="MQLTASKPDRCICCLAIALTGFISIDPVEPKEMLIFEKDVRPIL
                     KAYCLDCHGGGDALKGHLDLRLKRFAERGGDSGPALVAGHPEESLLIERLKNGEMPPG
                     EKKVPPEQIALIERWIAAGAVAKRNEPERLDPGIDMTPEERAYWAFQPIRRLAPPTAG
                     PSDRVRTPIDAFVLAKLRERGLSLAPDTDKRTLIRRAAFDLTGLPPSQEEIDAFVADS
                     VDGAYERMLDQLMASPRYGERWARHWLDVTGYADSDGNGTEDSIRPYAYKYRDYVVRS
                     MNADKPLDQFIVEQLAGDELVPRPWNELSREKAELLAATGFLRTAPDGTSTGASDEPL
                     AANQVVADTLKIVGSTLLGMTVGCAQCHDHRYDPIPQADYYRLRAIFEPALDTNHWRR
                     PAQRLVSLFKEADRARCNAIEAEAAKLEKIVAAKTKTYMEAAIKKELAKFPEALRSKL
                     RAALDVAPASRSDEQRRLLASNPSVNITEGVLYQYDPAAAEDLKKETGKVAAKRAEKP
                     IEDFVSVLDEVPGVLPTTKIFHRGDHRQPTKTVRPGDLSIAAPEGKRYEAPEKDAALP
                     TSGRRLAYARHLVTGDHPLVARVLVNRIWLSHFGRGLVDTPGDFGVLGMRPTHPELLD
                     WLADQLVRQGWSLKRMHKLIMTSTVYRQSSRGTQSGSALDSSNTLYSHFPLRRLEAEE
                     IRDVILHLSGRLAITLYGPPVPVAEDTVGHILPDKDSARRSLYLQSRRTKPVSLLATF
                     DFPTMAVNCDRRATSTSATQSLVLMNSEFVLSHAGHVARRVRQLTSAGSASDQLAREA
                     TCAWKLVYQRAITPEERAWVNEYVSRQLSTSGPDRELAVLTNLCQQLLISNEFLYVD"
     gene            3031..4485
                     /locus_tag="VHS97_08545"
     CDS             3031..4485
                     /locus_tag="VHS97_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3448292.1"
                     /translation="MSTESPFPTRRDFLARTAFGVGAIALADLLRQDGALGDVAKKPG
                     ENLPLDLRARAPHFAPKATAMISLFMHGGPSHVDLLDPKPELSKRNGTEYDGDVVFSF
                     VKRASKQLFGTPWRFARHGQCGTEVSELLPETAGIVDDICVIRSMHTGHNGHEVSIRF
                     FHGGIAGVTGRPTLGSWLTYGLGSESEELPAYMILSDPAGHPVDGTNNWSSGFMPTMY
                     QGTVLRPQEPRILNLDPPAHLRGEPQRQNLSLLDRLNRRHFESHPGESDLETRIRSYE
                     LAAAMQTTAKEALEIGNEPAHIKTLYGLDEDHTREYGTRCLIARRLVERGVRFVQIFL
                     GGQPWDTHTSIKSALPAICRRTDKPAAALVKDLKQRGLLDTTLVHWGGEIGRLPVTEG
                     NADASAGRDHNGQGFSAWLAGGGIKGGMTYGATDEVGHRAVENIVTPNDFQATVLQLF
                     GLDHNRLVYHANGREQRLIDGRNARIVKEILCSG"
     gene            4687..5136
                     /locus_tag="VHS97_08550"
     CDS             4687..5136
                     /locus_tag="VHS97_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX3448293.1"
                     /translation="MRFQVLGLMFVSALLMVAADDPKDAVKKEYDKFEGTWKMESFTV
                     DGKPTPIETFADFRMTLKGENFTTVTAEGKTNGTYKVDPSKSPKTIDITFTGGPLDGV
                     TMLGVYKLDGDTYTVCLPAGGGKERAKELASKPGSGLVLEVLKRVKP"
BASE COUNT         1137 a         1694 c         1616 g         1026 t
ORIGIN      
        1 ccgatcttca gaaagtggcg ccgggaatgg ccatcgcaat acgccgtgga tttctggcca
       61 agaatggaaa gcatggcgcg gttctctcgt ggacggctgg ccactgtcgg ctacaaaccg
      121 gcaggtttga ccaggactgc atggaggaca ttcatgtcta attgccaacg ggcatctgcg
      181 gcgacacgag atcctgtcat ggatctccaa ttccatttgt caggctaaaa gagaattacc
      241 cccagtgccg ggcgtgagag gttggccagg ccgctacatt gcgcaaggcc ccacgaacgc
      301 cgcatgcatc acgcgaggtt cgtaattcat tgtaggtcgc tgttgaacgc ttgtcaaaca
      361 agcggttggt ccgggaaaaa tgcgccttgt ttgctgatca cggttgaaca ataatttgac
      421 aaacaccgtg gaacaatcgc cgacattccc cgcagccgag agcgcaatcg atcatgcagc
      481 tcactgcctc gaaacccgat cgttgtatct gctgcctggc tatcgcactc accgggttca
      541 tcagcattga tccggtcgaa ccgaaagaaa tgctgatctt tgagaaggat gtccggccga
      601 tcctcaaggc ttactgcctc gattgtcacg gcgggggcga cgcattgaag ggacaccttg
      661 acctgcgtct caagcgtttt gcggaacgcg ggggcgatag tggcccggcg ctcgtcgcag
      721 gtcatcccga agagagtttg ctcatcgagc ggctcaagaa cggggaaatg cctccggggg
      781 agaagaaggt gcccccggaa cagatcgctt taatcgagcg atggatcgcc gcaggggctg
      841 tggcaaagcg gaacgaaccc gaacgcctcg atcccggcat cgacatgacg cccgaagaac
      901 gcgcttactg ggcgtttcag ccaatccgcc ggttagcgcc gccgacggct ggaccgagcg
      961 atcgggtgag gacgccgatt gacgcatttg tcctggccaa gctgcgtgag cgcggtcttt
     1021 cgttggctcc cgacaccgac aagcggacct tgatccgacg ggcggccttc gacctcacag
     1081 gcctgccccc ttcacaagaa gaaattgacg cgttcgtcgc ggactcggtt gatggcgcgt
     1141 acgaacgtat gctcgaccag ctgatggcct cgccccgcta tggcgagcgt tgggcgcgac
     1201 actggctgga cgtgaccggg tatgccgact cggatggtaa cggtaccgag gactcgatac
     1261 gaccttatgc ctacaaatac cgtgactacg tggtgcgctc aatgaatgcc gacaagccgc
     1321 tcgaccagtt catagtcgag caacttgccg gtgacgagct ggtccctcgt ccctggaacg
     1381 agttgagccg ggaaaaagcc gagttgctgg ccgcgacagg gtttctgcga acggcgccgg
     1441 acggaacttc aacaggcgcg agcgatgagc cattggctgc gaatcaagtg gtggccgaca
     1501 cgctgaagat cgttggctca acactactgg gcatgacggt gggctgtgcc caatgtcacg
     1561 accatcgcta cgacccgatc ccccaggccg actattaccg gctccgagcc atcttcgagc
     1621 cggcacttga caccaatcac tggcggaggc ccgcgcagcg gttggtctct ctattcaagg
     1681 aagccgatcg ggcgaggtgt aatgccattg aggccgaggc cgcaaaactt gaaaagattg
     1741 ttgccgccaa gaccaaaaca tacatggagg cggcgatcaa gaaggagctg gcgaagtttc
     1801 ccgaagcgct tcggtccaag ctccgcgcgg cccttgatgt cgctcccgcg tcccgatctg
     1861 acgagcagag gcgtctgctc gcatcgaacc ccagtgtgaa tatcaccgaa ggggtcctgt
     1921 accagtacga cccggccgct gcggaggacc tcaaaaagga aaccggaaaa gttgctgcca
     1981 aacgcgcgga gaagccaatc gaagattttg tcagcgtgct cgatgaggtt ccaggagtgt
     2041 tgccgacgac gaaaatcttc catcgcggcg accatcgtca gcccactaaa actgtccgac
     2101 cgggtgacct cagcattgcc gcgccagaag gcaagcgata cgaggccccg gaaaaggacg
     2161 ccgctctgcc cacctcgggg cgccggctcg cttacgcacg gcatctcgtc acgggagacc
     2221 atcccctggt ggctcgggtc ttggtgaatc ggatctggct gagtcatttc ggtcgtggcc
     2281 tggtcgatac gccgggcgac ttcggtgtcc tcggcatgcg ccccacccac ccggagttgc
     2341 tggactggct tgccgaccag ctcgttcgcc agggatggtc tctcaagcgt atgcacaagc
     2401 tgattatgac ctccacggtt tatcggcaat cttcacgtgg gactcagtcg ggttcggccc
     2461 ttgattcgag caacaccctt tacagccact tccctcttcg gaggctcgaa gccgaagaga
     2521 tccgcgacgt cattctccac ctgagcggcc gcctcgccat tactctgtac gggccgcctg
     2581 tgccggttgc cgaggacacc gtcggccaca ttctgcctga caaagactcc gcaaggcgca
     2641 gtctttacct tcagtcgcgc cggacgaagc cggtttcgct gctggcgaca ttcgactttc
     2701 cgacgatggc ggtaaactgc gaccggaggg ccacgagcac gtcggccacg cagtccctcg
     2761 tgctcatgaa tagcgaattt gttctgagcc acgccggcca cgtggcccgg cgcgtacgcc
     2821 agcttacttc cgcaggctcg gcttcggacc aactcgcgcg agaagccacc tgcgcgtgga
     2881 agctcgttta ccagcgcgcg atcacacctg aagagagggc atgggtgaat gagtatgttt
     2941 cccgccaact ctcaacgtct ggtcctgatc gcgaactcgc cgtgttgacg aatctctgcc
     3001 agcaactcct gatttccaac gagttcctct atgtcgactg aatcgccctt tccgacccgg
     3061 cgtgattttc tggcacgaac cgccttcggg gtcggcgcaa tcgccctggc cgacttgcta
     3121 cgccaggacg gagcgctcgg tgacgttgcc aagaagcctg gcgaaaatct ccccctggac
     3181 ctgcgggcac gagcgccgca tttcgctccc aaggcgacgg ccatgatctc gctcttcatg
     3241 cacggtggtc cgtcgcatgt cgacttgctt gacccgaagc ctgagttgtc gaaacgaaac
     3301 ggaaccgaat acgacgggga cgtcgtcttc agtttcgtca agcgcgcgag caagcagctc
     3361 tttggcactc cgtggaggtt tgccaggcac ggccagtgcg gcaccgaggt ctcggaactg
     3421 ttgcccgaaa cggccggtat cgtcgacgac atctgcgtga ttcggtcgat gcacaccgga
     3481 cacaatggcc acgaggtttc gattcgtttc tttcacggcg ggatagccgg tgtgactggc
     3541 cggccgacgc tagggagctg gctcacgtat ggtctcggaa gtgagtcgga agagctcccg
     3601 gcctacatga ttctttccga ccccgcggga catcctgtcg acgggacgaa caactggtcg
     3661 agcgggttca tgcctacgat gtatcaaggg accgtgctcc ggccgcagga gcctcggatc
     3721 ttgaatctcg accctccggc ccatcttcga ggagaaccgc agcgccagaa tctctcgctt
     3781 ctcgaccggc tcaatcgcag gcacttcgaa agccatccag gcgaatcgga cctcgaaacc
     3841 cggattcgca gctatgaact tgctgcggcc atgcagacga ccgcaaaaga ggcgctcgaa
     3901 atcggcaacg agcccgctca catcaagact ctctacggat tggatgagga ccatacgcgc
     3961 gaatacggca cgcgctgcct gatcgcccgc cggctggtgg aacgaggcgt gcggttcgtc
     4021 caaatcttcc tcggcggcca gccctgggac acccacacca gcatcaagag cgcactgccg
     4081 gcgatctgtc gccgaaccga caagccggcc gcggcccttg tcaaggacct caagcagcgc
     4141 gggctgctcg acacgaccct cgtccattgg ggtggagaga tcggccggct tcccgtcacc
     4201 gaaggcaatg ccgatgcctc cgccggtcgc gaccacaatg gacagggctt ctcggcctgg
     4261 ctcgcgggcg gcgggatcaa gggaggcatg acctacggag ccaccgacga agtgggtcac
     4321 cgcgcggtcg aaaatattgt gacccccaac gacttccagg cgaccgttct gcaacttttc
     4381 gggctcgacc acaatcgact cgtctatcac gccaatggtc gagaacagcg gctgattgat
     4441 ggtcggaacg cacggatcgt caaggaaatt ctttgctcgg gctgagacac tcgccttcaa
     4501 attcgtgcag gtttgcttcc gcactctact acgccgccga tcgaaccccc ctccgcgccg
     4561 caccgacctc cgcatcccga tagctgtagg aattcggccg ccgtcttccg ctccgcacac
     4621 cgcccggata ccctggactg gtcgtcgttt tctccccgta gatctcgagg aatggagagg
     4681 tacgccatgc ggttccaggt tctcggactg atgttcgtgt cggcgctcct gatggtggct
     4741 gcggatgacc ccaaggatgc ggtcaagaaa gagtacgaca agtttgaggg cacgtggaag
     4801 atggagtcat tcaccgtcga cgggaagccc acgccaatcg aaaccttcgc cgacttccgg
     4861 atgaccttga aaggcgagaa cttcaccacg gttaccgcag agggaaagac gaacggcacc
     4921 tacaaggtcg acccgagcaa gtcacccaag acgattgaca tcacgttcac cggcgggccg
     4981 ctcgacggcg tgacgatgct aggcgtctac aagctcgacg gcgataccta tacggtttgc
     5041 cttcccgccg gcggtggcaa ggagcgcgcg aaggagctgg ccagcaagcc aggctcgggt
     5101 cttgtgctcg aggttttgaa gcgggtcaaa ccctgatcga acgagctcag ctcaacagca
     5161 acgcgaccct ggcggcgaac gtgcccgctc acgtcgtcac cactacggac ggcgccaacc
     5221 tcaacctgaa cggccaccct aagtgagacc gcagaactat ttgtgtaggt cgggctttcc
     5281 agcccaacac tttgtgccct ccagcaaaac gtcgggctag aaagcccgac ctacgctgcg
     5341 cgacgacttc ggcgggtcca cttagacggc tgcgtcgtct ggaggatgca tcgcaatcgt
     5401 caggctggaa ttaagtgaca ccgctcatgt catcgcgaat cagctcatcg accggaaagc
     5461 ggaagccgcc gtt
//
LOCUS       DATAQL010000498         8352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1270323, whole genome shotgun sequence.
ACCESSION   DATAQL010000498 DATAQL010000000
VERSION     DATAQL010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270323"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="VHS97_08555"
     CDS             <1..1836
                     /locus_tag="VHS97_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3448294.1"
                     /translation="FQPGSQLLASGGDDGKILIWDTRTGSLVRALHIKKDTIYALNYS
                     PDGRLLASGHGYPPIEEVDHMRGRGVVSVWDAATGQVLCTLRGHTQNVMGVAFSPDGR
                     TLASVSGSSLAVPQAASKPGELCLWTVETGALVRCQTAHAAPLTGVSYHPSGDLLATS
                     SWDRTIRLWDSRTGDLRQSLSGHKDWVLHVAFSPDGKRIATGGADGAIKIWDTATTQE
                     LWTLRGHTKNVTCVAFRPDGRRLASSSSDQTIKIWDGTASPEALTWRGAVGPIARIAY
                     FPDGNHVLVAENVEDDKGSVRDRLTILDTAHDMRDVILDDATDSESRRPIEGIAIRRD
                     GRYVASASHSGRIEAWTVPDGRSCFRYDESTSRFEDVAFSPNGRRLAASGQVRARLPD
                     GEATPNDSDSNGLLLVFDLETGTTRWRVAGMKTGIIRDLAFSPDGETIATADNFCTIT
                     IWDSSTGKALSQLRGHDRLVSRVAFSPDGKKLASASWDSTVVVWDLASRQPTTRLQGH
                     MRSVLCVVFSPDGRRLATSSEDQTVKLWDVDTGQEVLTLHGHTDIVPTVAFSPDGNRL
                     ATAGADGVVLIREASPAHQSGLPPYPTDISGEPTALKGQTAMTRP"
     gene            2114..2186
                     /locus_tag="VHS97_08560"
     tRNA            2114..2186
                     /locus_tag="VHS97_08560"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2147..2149,aa:Thr,seq:tgt)
     gene            2290..2371
                     /locus_tag="VHS97_08565"
     tRNA            2290..2371
                     /locus_tag="VHS97_08565"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2324..2326,aa:Tyr,seq:gta)
     gene            2587..2657
                     /locus_tag="VHS97_08570"
     tRNA            2587..2657
                     /locus_tag="VHS97_08570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2619..2621,aa:Gly,seq:tcc)
     gene            2808..2880
                     /locus_tag="VHS97_08575"
     tRNA            2808..2880
                     /locus_tag="VHS97_08575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2841..2843,aa:Thr,seq:ggt)
     gene            3038..4249
                     /gene="tuf"
                     /locus_tag="VHS97_08580"
     CDS             3038..4249
                     /gene="tuf"
                     /locus_tag="VHS97_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX3448295.1"
                     /translation="MAKETFSRTKPHVNVGTIGHIDHGKTTLTAAILAVLAVQGKAKT
                     KSYADIAKGGTVRDSTKTVTIAVSHVEYESEKRHYAHIDCPGHADYIKNMITGAAQMD
                     GAILVVSAADGPMPQTREHILLAHQVGVPALVVFLNKIDLVDDEELLELVELELRELL
                     THYKFPGDEIPIIRGSSRPALENPSDAKAAKPILDLVKAMDEYIPEPVREIDKPFLMP
                     IEDVFSIKGRGTVGTGRIERGKVKVGDPVEIIGFGVKKASTITGVEAFQKTLDEGMAG
                     DNVGVLLRGVEKEDLERGQVICKPNSITPHSKFEAEVYVLSKEEGGRHTPFFKGYRPQ
                     FYFRTTDVTGSVLNLLSEDNTEAEMCMPGDNIKMTVELQTSIAMQESLRFAIREGGKT
                     VGAGVVTKILE"
     gene            4416..4568
                     /gene="rpmG"
                     /locus_tag="VHS97_08585"
     CDS             4416..4568
                     /gene="rpmG"
                     /locus_tag="VHS97_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX3448296.1"
                     /translation="MREYVWLECTTCGERNYRVQKETRGANRLELKKYCRRERTHTPH
                     KESRKK"
     gene            4706..4778
                     /locus_tag="VHS97_08590"
     tRNA            4706..4778
                     /locus_tag="VHS97_08590"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4739..4741,aa:Trp,seq:cca)
     gene            5007..5561
                     /gene="secE"
                     /locus_tag="VHS97_08595"
     CDS             5007..5561
                     /gene="secE"
                     /locus_tag="VHS97_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX3448297.1"
                     /translation="MGKLKDEVSSSKPSEPSKGARKSGMASKGKPGGAPPGQFTLFLV
                     NLARADLYKPMQGWYARLYTALGLGLIVATGAWRVYESSIEYSTYWHIGLPALFAAVL
                     GWVIFRLIHFPPFAEFLIATEAEMNKVSWTNKEDLFRATTVVLTTVVMMTVFLFLVDT
                     LWTFVLRMIGVLQFGGSGGFGSTA"
     gene            5666..6442
                     /gene="nusG"
                     /locus_tag="VHS97_08600"
     CDS             5666..6442
                     /gene="nusG"
                     /locus_tag="VHS97_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX3448298.1"
                     /translation="MSHPTQQAAGPPPPEGTDPETPKEAVASNPGIEVNEQTPDGGQL
                     GDKPHSEPAPAAESPGSAPSAAQSPGLPLDDDDDPPPELVWYVLKVQSSREDTIRDAL
                     QRRVKIQGLGRFFGQIVVPTEKITEIRNNKKRIVERKTYPGYIMVQMELNEKTWFLVR
                     ETPGVGDFVGAHGTPTKMTETEVNQMLHQEEEKTTAEVPKVRIYVERGDRVKIKDGPF
                     ENFEGTVEEVIEGRGLVKVMLIIFNRPTPVDLEYWQVERI"
     gene            6467..6889
                     /gene="rplK"
                     /locus_tag="VHS97_08605"
     CDS             6467..6889
                     /gene="rplK"
                     /locus_tag="VHS97_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX3448299.1"
                     /translation="MAKVMTAKVKLQCPGGQATPAPPVGPALGQHGVNIGQFVMQFNE
                     RTKEMKGTTIPVEITIYSDRTFEFVTKSPPAAVLLKQAAGIAAGSAVPNKTKVGTVTP
                     DQVKKIAETKMADLNARDLEHACRVIAGTARSMGVEIK"
     gene            7021..7701
                     /gene="rplA"
                     /locus_tag="VHS97_08610"
     CDS             7021..7701
                     /gene="rplA"
                     /locus_tag="VHS97_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX3448300.1"
                     /translation="MAQHSKRFKALTDKIKTTGPVPVPDAVRLLKNFNTTKFNQTVEV
                     STHLGIDPKQTDQNVRGSVALPHGIGKSVRVAVFAQGDNAEKARAAGADIVGADDLAQ
                     QIKGGTMDFDVALATPDMMGVVGPLGRVLGPRGLMPSPRSGTVTTDITSAVREFKAGK
                     IEFRNDKGGNVAVPVGKIGFTEDQLIENINAFLNYLRSLKPATAKGTYIRSITVSATM
                     SPGIRVSA"
     gene            7831..>8352
                     /gene="rplJ"
                     /locus_tag="VHS97_08615"
     CDS             7831..>8352
                     /gene="rplJ"
                     /locus_tag="VHS97_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX3448301.1"
                     /translation="MSKYVKELMMDQLRSDLDGNRSLLILDLKDLDAVAEHGLRRDLR
                     KKSIHLRVLKNSLARRVFADLGMDGLSRFLEGPSAMAWGGEGITELAKEISSQVKTLK
                     KPAIKGGVVEGVVVGPEQVDDITKLPSREVLIGQVIALLLGPAREALSLLSSGGSTLV
                     GQLEALAKRQQEAD"
BASE COUNT         1809 a         2401 c         2545 g         1597 t
ORIGIN      
        1 ttccagcccg gaagccagtt actggcgtcc ggaggcgatg acggaaaaat cctgatctgg
       61 gacactcgaa ctggatcgtt ggtgcgggcg ctccacataa aaaaggacac catttatgct
      121 ttgaattaca gcccggacgg gcggcttttg gcctcgggtc atggatatcc gccgattgaa
      181 gaggtcgatc acatgcgcgg tcggggcgtt gtcagcgtct gggatgcggc gaccggacaa
      241 gtgctctgca cgctgcgcgg acacactcaa aacgtcatgg gtgtggcgtt tagcccggat
      301 ggccgaacgc tcgcatccgt cagcggctct tccctggccg tgccacaggc tgcaagcaag
      361 ccaggtgagc tgtgcctttg gaccgtcgaa acaggcgcgc tggttcgctg ccaaaccgcg
      421 cacgccgcac cgctcacggg cgtcagctat caccccagcg gagatctgct tgcaacctcg
      481 agctgggacc gcacgatcag gctttgggat tctcgcaccg gggatttgcg gcagtcgctg
      541 tcgggccata aggactgggt tcttcacgtg gcgttcagcc ccgacggcaa gcggatcgcg
      601 accggtggtg ccgacggagc aatcaagatc tgggacaccg cgaccaccca ggaactctgg
      661 actttgcgcg gtcatacaaa gaacgtcact tgtgttgcgt tccgtcccga cggacgccga
      721 ctggcgtcga gcagctcgga tcagacaatc aagatctggg acggtacggc aagccccgag
      781 gcgcttacct ggcgaggcgc agtcgggccg atcgcgagaa tcgcttactt ccccgatggc
      841 aatcatgtgc ttgtcgccga aaacgtcgag gacgacaagg gcagcgttcg tgaccgcttg
      901 acaattcttg acacggccca tgacatgcgt gacgtgatac tcgatgacgc cacagattcc
      961 gaaagccgcc gaccgattga aggaattgcc atccggcgtg atggccggta cgtcgcgtcg
     1021 gcgtcgcata gcggacgcat cgaggcgtgg acagtcccgg acgggcggtc gtgttttcga
     1081 tatgatgaat cgacaagtcg attcgaggac gtcgccttta gcccgaatgg acgaaggctc
     1141 gccgcctcgg gtcaggttcg tgcccgactg cctgacggcg aagccacccc caatgactcg
     1201 gattccaacg gccttctcct cgtgtttgac ctggaaactg gaacgacccg ttggcgggtc
     1261 gcgggtatga aaacgggaat tatccgcgac ctcgcgttta gccccgatgg cgaaacgatt
     1321 gccacagcgg acaacttctg cacgattaca atttgggata gtagcacagg gaaggcgctc
     1381 agccagctcc gcggccatga tcggctggtt tctcgcgtcg cattcagccc agacggaaaa
     1441 aaactcgcat cagcgagctg ggattcgacc gtcgtcgtct gggatctcgc ttccaggcag
     1501 ccgaccacgc ggcttcaggg ccacatgcgg tcggtgttgt gcgtggtgtt tagccccgac
     1561 ggccgccggc tggcaacttc cagcgaggat caaacggtca agctctggga cgttgacact
     1621 gggcaggagg tgttgaccct gcacggccac accgacatcg tgcccactgt cgccttcagc
     1681 cccgatggga accggctagc gaccgcgggc gccgatggtg tcgtcctgat ccgagaggct
     1741 agccccgcgc atcaatcagg attgcccccc tacccgaccg atatttctgg ggagccaact
     1801 gccctcaaag ggcagaccgc gatgaccagg ccttgagttc acggatgacc gctctgacag
     1861 ccccgaccag acttttcccg aaattcggcg aatgggagcg ggtaaactgc ttgccgtatc
     1921 tctgccaaca acctcttgcg cctataacct cttgcccaaa gtaggacgga atcgctatga
     1981 tctaactatt aacctgccgc gctctggctg cgctcgcgat caaagaatat tagaccccat
     2041 tttcgtcgtc gtgcggaaag gccttgcggc gatgcgcgga agaggtatat tagaaaattt
     2101 cacggtccag gttgctggcg tagctcaatc ggcagagcat cggttttgta aaccgagggt
     2161 tgagagttcg agtctctccg ccagctctgt gatccgattt gtcggcaaag tttccacgaa
     2221 attcctcttg gcgagatagg gcgtgggttt cggcacaccg gtggaaatga cggaaacctg
     2281 gcaccaagcg ggtggatacc caagcggcca aaggggccag actgtaaatc tggtggcact
     2341 gccttcgcag gttcgaatcc tgctccaccc atgctggtcc cgaatgggat ggaagcggat
     2401 ctgagaatcg cgtttggtct tcatggggct gccggaaagt cgaaagaagt tcaacatgga
     2461 gcggtacgac aacgaccgct ggggatattc accaaagtga tttaaactgg aaatgaacgg
     2521 ctcgctcggc ctttgcgacc tgcgatgaat cctcccgggc gccggctcgg acgccgacga
     2581 aaaaacgcgg gtgtaactca atggtagagt acctgccttc caagcaggct acgtgggttc
     2641 gattcccatc acccgctttc acttggggtg gacagcaggg cctgggagga atttttttga
     2701 gccgctcggt ttcgtgagat cggtacgatt ggtttgatga cactctcgag cggcgaacac
     2761 gcctggtgcg aaccgggtct ggtagttggt gacatgactg acaatctgct gccgtggctc
     2821 agttggtaga gcgcgtcctt ggtaaggacg aggtcctggg ttcaaatccc agcggcagct
     2881 ttgggattga cgcgccggac cgtaagatta agtgggtatc atgatgacgt cggccgctgc
     2941 actgggtctg tctctggccg gggcagcgat tcgcaccagg ttttgccggg cagccctcca
     3001 gctcgccccc agtttatagg agacgacggg agtaacgatg gccaaggaaa ctttctccag
     3061 aaccaagccg cacgtcaacg tgggcacaat cggacacatt gaccacggca agacgaccct
     3121 gacggccgcc atcttggcgg tcctggccgt gcaaggcaag gccaagacca agtcgtacgc
     3181 cgacatcgcc aagggcggca ccgttcgcga ctcgaccaag accgtgacga tcgccgtctc
     3241 acatgtcgag tacgagagcg aaaagcggca ttacgcccac atcgattgcc cgggacacgc
     3301 cgactatatc aagaacatga tcaccggcgc ggctcagatg gacggcgcga tcctggttgt
     3361 ctccgcagcc gacggaccaa tgccccagac ccgcgaacac atcctgctgg cacaccaggt
     3421 tggtgttccc gcgctcgtgg ttttcctcaa caagatcgac ttggtcgacg atgaggagct
     3481 gcttgaattg gtcgagctcg agctccgcga gctgttgacg cactacaagt ttcccggcga
     3541 cgagatcccg atcattcgcg gcagcagtcg tcccgcactc gagaacccca gcgatgccaa
     3601 ggccgccaag cccatcctcg accttgtcaa ggcgatggat gagtatattc ccgagccggt
     3661 gcgcgaaatt gacaagccgt ttttgatgcc gatcgaagac gttttctcga tcaaggggcg
     3721 gggtaccgtc ggcaccggcc ggatcgagcg cggcaaggtc aaggtgggtg atcccgtcga
     3781 gatcatcggt ttcggcgtca agaaagcctc gacgatcacc ggagtggagg catttcaaaa
     3841 gaccctcgac gaaggaatgg ccggcgacaa cgtgggcgtg ctgctccgtg gcgttgagaa
     3901 agaagatctg gagcgtggac aggtcatctg caagcccaat tcaatcaccc cgcacagcaa
     3961 gttcgaggcg gaggtttacg ttctcagcaa ggaagaagga gggcggcata cgcctttctt
     4021 caagggatat cgcccgcaat tctatttccg cacaaccgac gtgacgggtt cggtcctcaa
     4081 cttgttgtcg gaagacaata ccgaggccga gatgtgcatg ccgggcgata atatcaagat
     4141 gactgtcgaa ctccagacat cgatcgccat gcaggagagt cttcgttttg ccatccgcga
     4201 gggcggtaag accgtcggtg ccggcgttgt gaccaagatc ttggaatgat gcgcggctcg
     4261 aatagggcca tacacagtga cgggtgttct gggagcgctc ggcattttgg gcacgtaagt
     4321 ttagtcaaaa atcgacccct gacggctcag ccggaaatcc gcagccgcca aggggttttg
     4381 caaattaact tccggcttag ggcaggagca agcggatgcg ggaatatgtc tggctcgagt
     4441 gcaccacgtg cggcgaacgc aactatcggg tgcagaagga aacccgggga gcgaaccggc
     4501 tcgagctcaa aaagtattgt cggcgcgagc ggactcacac cccgcacaag gaatcgcgca
     4561 aaaagtaagt gatcgagaac gtgccggcgg gacgccgctg gccggcacgg cgttgatggg
     4621 cggctttgac cgcgagttcc cgcgtgctgg ccgtccgatc tatttagagt tgcgaagatg
     4681 agtgatttat ggcggttcgt gagatacgag cgtagctcaa ttggtagagc accggtctcc
     4741 aaaaccggcg gttgggggtt cgattccctc cgctcgtgct gtcgcgaggc ggttttccag
     4801 tccgccgcga agttcgatgc ggaccccgtt acctctttaa tctaacgtgc cctaattgcc
     4861 attgccggtc gagtctctgt tcgaaaccaa accgcggtcg gagccaacgc atcgatcgat
     4921 cacagacagc cgggcatgat cgctgagttc gcttatccct cccagaatct gagactgtac
     4981 ccgttcaagc atgcgagaca ggtgccatgg gcaaactaaa agacgaagtg tcgtcgtcga
     5041 agccatcgga gcccagcaag ggtgccagga aatcaggtat ggccagtaag ggcaaacccg
     5101 gtggcgctcc tccaggtcaa tttaccttgt tccttgtcaa tctggctcgt gccgatttgt
     5161 ataaacccat gcagggttgg tatgcgcgac tctataccgc gctcgggctg ggtctgatcg
     5221 tggctactgg cgcctggcgg gtctatgagt catcgatcga gtattcgaca tactggcaca
     5281 ttggcctgcc ggcgctcttt gctgccgttt tgggttgggt catttttcgt cttattcatt
     5341 tccccccgtt cgctgaattt ctgatcgcga ccgaggccga aatgaacaag gtctcttgga
     5401 ccaacaagga agacttgttc cgcgcaacca ctgtggtatt gacgacggtg gtcatgatga
     5461 cggtttttct gttcctggtt gacacgcttt ggaccttcgt gctgcggatg atcggtgtcc
     5521 tgcagtttgg cggcagcgga ggattcgggt cgacggccta aacgcgcgag gccgcacggg
     5581 acggtgccct aaaaacagcg ccgcctgtgt ggccacgatc gaacttcaac gggatttcgc
     5641 acgcctgaca cgactagatt tcactatgag ccacccgacc cagcaagccg ccggtccacc
     5701 accccctgaa ggcaccgatc cggagacacc gaaggaggca gttgcctcca atcccgggat
     5761 cgaggtcaac gaacagactc ccgacggcgg tcaactgggc gacaagcccc actcggaacc
     5821 ggcgcctgcg gcagaatcgc caggcagcgc accgtcggcc gcccaatcac ctggtctccc
     5881 gctcgatgac gacgacgacc caccgcccga actggtctgg tacgtgctca aggttcaaag
     5941 ctcgcgcgag gatacgatcc gtgatgcact gcagcgccgg gtcaagatcc agggtctcgg
     6001 gcggtttttc ggccagatcg tcgttcccac cgagaagata accgagatcc gcaacaacaa
     6061 gaaacggatc gtcgaacgca agacttaccc cggttacatc atggtccaga tggaattgaa
     6121 cgagaagaca tggttcctcg ttcgtgagac cccgggtgtc ggcgactttg tcggggctca
     6181 cggaaccccg accaagatga ctgaaaccga agtcaaccag atgctccacc aggaagagga
     6241 gaaaacgacc gccgaagttc ccaaggtccg catttatgtc gagcgcggcg atcgggtcaa
     6301 gatcaaggac ggaccgttcg agaatttcga gggtaccgtc gaggaggtca tcgagggccg
     6361 cggcttggtc aaggtgatgc tgatcatttt taaccgcccc actccggtcg acctcgaata
     6421 ctggcaagtg gaacgaattt agagaagaga agagagagac gcaacgatgg ccaaggtgat
     6481 gacggcgaag gtaaaactgc agtgccctgg tgggcaagcg accccggctc cccccgtcgg
     6541 ccccgcgctg ggccagcacg gggtcaatat cggtcagttc gtgatgcagt tcaacgaacg
     6601 caccaaggag atgaagggga cgaccatccc ggtcgagatc acgatctact cggatcgaac
     6661 cttcgagttc gtgaccaaga gtccccccgc cgccgtgctc ttgaagcaag ccgccggtat
     6721 tgccgccggc tcggccgtcc ccaacaaaac caaggtcggc actgtgacgc ccgatcaggt
     6781 caagaaaatt gccgaaacca agatggccga cttgaatgcc cgcgacctgg aacacgcctg
     6841 ccgggtcatc gccggcacgg cgcggagcat gggagtggaa atcaaatagc aggcagtacg
     6901 ccgtaggcat taggcagttc ggtgaacgcc gaacacgttg gctcatcacg cgaccgccca
     6961 tgatgagtcc gatcgcgtac aaggcggcag ctccgtagtt tttgaaagga gcgtgagcaa
     7021 ttggctcagc attcgaagcg gttcaaagca ttgactgaca agatcaagac gacgggtcca
     7081 gttcccgtgc cggacgccgt ccggctattg aagaacttca atacgaccaa attcaaccag
     7141 actgtcgagg tctcaaccca cctggggatt gatccgaagc agaccgacca gaacgtgcgc
     7201 ggctcggtcg cgctgccgca cggcatcggc aagtcggttc gagttgccgt cttcgcccag
     7261 ggcgataacg ccgagaaggc gcgagccgcc ggggccgaca tcgtgggggc agacgacctg
     7321 gcccaacaga tcaaaggggg gaccatggat ttcgacgtgg ccctggcgac acccgacatg
     7381 atgggagtcg tcggaccgct cggccgcgtg ctaggtcctc ggggccttat gccctcgccc
     7441 cgctcgggaa ccgtcaccac cgacatcacc tcggccgtgc gcgaattcaa ggcgggcaag
     7501 attgagtttc gtaacgacaa gggaggcaat gtcgcggtgc cggtcggaaa aatcggcttc
     7561 accgaggacc agttgatcga gaacatcaac gccttcttga actacctgcg ctcgctcaag
     7621 cccgcaaccg ccaaggggac ctatatccgc tcgatcaccg tctcggcgac catgagcccg
     7681 ggcatccgcg tttccgcatg aggcgcgggc ctcgcaaact ccctcgcagt cggagacgac
     7741 cgcggggaac tttgcgaatg atccattgga taagacaaat tctgatccga agacaaactt
     7801 atagaacatg cggggttaat agataagtcg atgagtaaat acgtcaagga actgatgatg
     7861 gatcagctgc ggtcggatct tgacggcaac cggtcgctgt tgatcttgga cttgaaagat
     7921 ctggatgccg ttgccgagca tgggctccgc cgcgacctgc gcaagaagtc gatccacctg
     7981 cgggtcttga agaactctct ggcccggcgg gttttcgccg acctgggaat ggacggactg
     8041 tcccgattcc tggaaggccc ctcggcgatg gcgtggggag gagaggggat caccgagctg
     8101 gctaaggaaa tttcatcgca ggtcaagacc ctcaaaaagc ccgcgatcaa gggaggggtg
     8161 gtcgaggggg ttgttgtcgg tcccgagcag gtcgacgaca ttaccaagct ccccagccgc
     8221 gaggtcttga tcggtcaggt gatcgcgctc ttgctcgggc cggcccgcga ggcgctatcg
     8281 ctcttgtcgt cggggggttc cacgctggtg ggacagctcg aagccctggc caaacggcag
     8341 caggaggcag ac
//
LOCUS       DATAQL010000499         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2340214, whole genome shotgun sequence.
ACCESSION   DATAQL010000499 DATAQL010000000
VERSION     DATAQL010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340214"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VHS97_08620"
     CDS             complement(<1..262)
                     /locus_tag="VHS97_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448302.1"
                     /translation="MHLDIGKTAIDSLNRRAWLGTTARALAGITCGAAVPRQAFGLDP
                     SVEAQAAIRGKPTRFQIACMTLPYSRFSLDRALTGIKAAGYDY"
     gene            complement(339..1619)
                     /locus_tag="VHS97_08625"
     CDS             complement(339..1619)
                     /locus_tag="VHS97_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448303.1"
                     /translation="MRHGSPSRRDFLTTAAAATTTAALSNAHAAGSDVLRIGLIGCGD
                     RGTGAATQALCAESNVKLVAMADAFADRLQKSLEILQKDEKVASKLDVKPDHRFVGFN
                     AYRELLEVGVDVVLLCTPPHFRPQHIQAAVEAGKHVFAEKPVAVDVPGVHSVLATCEL
                     AKKKGLSVVSGLCLRYDNGFREAVRRIQDGAIGEVVSLFANDYRSGRWTKPRQPDWTD
                     MLYQMRNWYNFTWLSGDFNVEQHVHFLDICAWVMKNQYPAKAVGMGGRQVLTEPEYGQ
                     IYDHFSVVYDYVDGPKLVSNCRQQPHCKNDMSVHVVGTRGRADLSERRKGLQIKTPAG
                     TWTYGGPQNQMYQTEHDELFASIRSGKPINNGDYMAKSTLLAIMGRMTAYTGQEITWE
                     MAQKSKENLSPSRYAWDARPPAAQIAVPGQTRFV"
     gene            complement(1633..>2631)
                     /locus_tag="VHS97_08630"
     CDS             complement(1633..>2631)
                     /locus_tag="VHS97_08630"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3448304.1"
                     /translation="RHSPGKLGSTSPTETSAMLRQHNPSHNSDHPSLAGVNSSRVKWQ
                     SGLPATEPRTRKEGGRQIARLCFCAALVLGQVGSGGLQGDEPPAKSVSPSEAVRLFNG
                     HNLSGLSTWLEDTKHDDPRRVFRVEDGLLHITGDGFGYLSTGKEYRDYHLVVEYKWGK
                     KTDGGKFVRNSGILLNAAGPDGGAHVKWMSSIECQLAQGCVGDLIAIRGVDAKGETIP
                     VQLTSETVLGPDKRPRWMKGGEPRVFTNQQLWWSRHDPDFEELLDTRGKNDVESPMGE
                     WTRVDCRCAGNRITVRVNGTTVNECYDVFPAAGKILLQSEGFELFVRKFELHPLQN"
BASE COUNT          472 a          811 c          810 g          538 t
ORIGIN      
        1 cgtagtcgta gccggccgct ttgattccgg taagcgcccg atcgagtgag aaacgagaat
       61 aaggcagggt catgcaggcg atctggaacc gcgtcggctt gccgcgaatg gccgcctgcg
      121 cctcgacgga tgggtccaaa ccaaacgcct gccgcggaac ggccgcgccg caggtaatcc
      181 cggctagcgc tctagccgtg gtacccagcc aggcgcggcg gttcagggaa tcaatggcag
      241 tcttgccgat atcaagatgc atgacatctc ccgcagaaag cgacccggac tgtggtcttt
      301 ctggccgacc atccgttcct tgtggcgcac cctgtttctc agacgaagcg tgtctgcccc
      361 gggacggcga tctgggcggc ggggggccgg gcgtcccagg cgtagcggct tggactcaag
      421 ttttcttttg acttttgcgc catctcccag gtgatctcct gaccggtgta agccgtcatg
      481 cggcccatga tcgcaagcag tgtgcttttt gccatgtagt cgccgttgtt gatgggcttg
      541 ccgctacgga tgctggcaaa gagctcatcg tgctcggtct gatacatctg gttctgaggc
      601 ccgccgtagg tccaggtgcc cgctggagtt ttaatctgaa ggcctttgcg tcgctccgac
      661 agatcggccc gaccccgagt tccgactacg tggacgctca tgtcattctt gcagtgcggc
      721 tgctgccggc aattgctcac aagcttcggc ccgtcgacgt agtcataaac gacggagaaa
      781 tgatcgtaga tctgcccgta ttccggctct gtaagcactt gccggccacc catgccgaca
      841 gccttggcgg ggtattggtt cttcatcacc caggcgcaga tatcgaggaa gtgaacgtgc
      901 tgctcgacgt tgaagtcgcc cgacaaccag gtgaagttgt accagtttcg catctggtac
      961 agcatgtcgg tccagtcggg ttgtcgcggt ttggtccagc ggccgctccg gtagtcgttg
     1021 gcgaagagcg agacgacctc gccgatcgcc ccgtcttgga tgcgccggac ggcctcccga
     1081 aagccattgt cataccggag gcaaaggccg gagacgaccg agaggccttt ctttttggcc
     1141 agttcgcagg tcgccaggac cgaatggacg cccgggacat cgacggcgac cggcttctcc
     1201 gcgaaaacgt gcttgccggc ctcgactgcc gcctggatgt gttgcgggcg gaagtgtggc
     1261 ggcgtgcaca gcagcaccac gtcaacgcca acttcgagca gttctcggta ggcattgaag
     1321 ccgacgaaac gatggtcggg cttgacatcc agcttgctgg ccaccttctc gtccttctgg
     1381 aggatttcca ggctcttctg tagccgatca gcgaaagcgt cggccatagc gacgagcttg
     1441 acattcgact cggcacaaag cgcctgggtg gccgcaccgg tgccgcggtc gccacagccg
     1501 atcaggccga tgcgcagcac gtcgctgccg gcggcgtgcg cgttcgagag cgcggccgtc
     1561 gtcgtggcgg cggccgccgt ggtgagaaaa tcgcggcgcg atggtgatcc gtgacgcatg
     1621 gcacagcctc tttcaattct gtaatgggtg caattcaaac ttccgcacaa acagctcgaa
     1681 cccctcggac tggagcagga tcttaccggc cgcggggaat acgtcgtaac actcgttgac
     1741 ggtcgtgccg ttgacgcgca ctgtgatccg gttccccgcg caccgacaat cgacacgagt
     1801 ccactcgccc attgggctct ccacgtcgtt tttgccccgg gtgtcgagaa gttcttcgaa
     1861 atccggatcg tgccgcgacc accagagctg ctggttggta aagacccgcg gttcgccccc
     1921 cttcatccag cgcggccgct tgtcgggccc cagaaccgtt tcgcttgtga gctgtacggg
     1981 tatcgtttcg cctttcgcgt ccacgccacg gatggcgatc aagtcaccga cgcacccctg
     2041 ggctagctgg cactcgatcg acgacatcca cttaacgtgg gctccaccat ccggcccagc
     2101 cgcgttcaag agaatgccgg agttgcgcac aaacttgccg ccgtcagtct tcttccccca
     2161 cttgtattcc acgaccaggt gataatcacg gtattcctta ccagtggaca ggtaaccgaa
     2221 gccgtcgcca gtgatgtgga gcaggccgtc ctcgactcga aagacccggc gtgggtcgtc
     2281 gtgcttcgtg tcctcgagcc aggtggagag gccggagagg ttgtggccgt tgaagaggcg
     2341 gacagcctct gacggactga cagacttggc cggcggctcg tcaccctgaa ggcccccgct
     2401 acccacttgg cccaaaacga gcgcggcgca aaaacacaat cgggcgatct ggcgacctcc
     2461 ttcctttcga gtccgaggtt cagtcgcggg gagcccggac tgccatttta cccgcgaaga
     2521 attcacgcct gctaggctgg gatgatcgct gttgtgactc ggattatgct ggcgaagcat
     2581 ggcgctcgtc tccgtcgggg atgtcgaccc aagttttccg ggtgaatggc g
//
LOCUS       DATAQL010000500         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2645308, whole genome shotgun sequence.
ACCESSION   DATAQL010000500 DATAQL010000000
VERSION     DATAQL010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2645308"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1172
                     /locus_tag="VHS97_08635"
     CDS             72..1172
                     /locus_tag="VHS97_08635"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3448305.1"
                     /translation="MAMGNRPEVKTASAPVVALDVGLLAQQLATAVARVPFRHPFRGD
                     SSPASNLMMSTTREVMRAFMGYSASLRIPEFRSLELVLDRICQVIMPPIVHSLEVESH
                     AVEYGGVPGILYLPAHRTTVGLIVYLHGGGYIGTSPTMYAFFTARVCHDTGCAVFVAD
                     YRLAPEFPYPAGAEDAASAYSDIVARGFPSERIFLAGDSGGGGLATTLILECPEHVLP
                     VRPAGLVLFSPEVDLRLNEPSITQNAESDILPWNIPTTAYLHGANATSNDLSPLTADL
                     GLFPPTCVTWGGDEMFRDPIRRFVDRLRAAGISTEAQEFPGMFHVFQILMPWAESSRS
                     AFRLVRDFIHKIVEDAPLIKDPRQIHPRLEAQ"
     gene            complement(1224..>1562)
                     /locus_tag="VHS97_08640"
     CDS             complement(1224..>1562)
                     /locus_tag="VHS97_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448306.1"
                     /translation="LDEMRTLLAARRLRPPHVAGAVAADVQGADAQARHPSKARRTAQ
                     RVLAEPADLLGAALAVSMRRAGELGEALTARGGVTTGVGTRPGPGVSDVVAGLVVAAT
                     CAAALALHLG"
BASE COUNT          264 a          515 c          489 g          294 t
ORIGIN      
        1 gggaacggct tggcggcgta ctctgagggt tgatctttaa tcgggtcagt attggaccgg
       61 aggcagcaac catggcgatg ggcaatcggc ctgaggtcaa gaccgcgtcg gcaccggtgg
      121 tcgcgttgga cgtgggtctt cttgcacagc aactggcgac cgcagtggcg cgtgtgccgt
      181 ttcgacatcc attccggggc gactcatccc cagcatcgaa tctcatgatg tcgacaaccc
      241 gcgaggttat gcgcgccttt atgggctatt ccgcctcact ccggattccg gaatttcggt
      301 cgctcgagtt ggttctcgac cgaatctgtc aggtgatcat gccgcccatt gtccacagcc
      361 tcgaagtgga gagtcatgct gtcgagtacg gcggtgtccc cggaatcctc tacctcccgg
      421 cccaccggac gacggtcggg ctgatcgttt atctccacgg aggcggctat atcggaacgt
      481 ccccaacgat gtatgccttc ttcacggcga gggtttgcca cgacacaggc tgtgccgtct
      541 tcgtagcgga ctaccgcctt gcaccggaat ttccgtaccc tgccggggcc gaggacgctg
      601 catccgcgta ttccgacatc gtggcgagag gtttcccatc tgaacggatc ttcctcgccg
      661 gcgactcggg gggaggcgga ctggcgacga cgctcatcct tgagtgccct gagcatgtgc
      721 tcccggttcg gcccgcggga cttgtgctgt tctcgcccga ggtcgacctg cggctcaatg
      781 agccgtcgat cacgcagaat gcggaaagcg acatcttgcc ttggaacatc ccgaccaccg
      841 cctatttgca cggagctaat gccaccagca acgacctctc tcctctcacg gccgatctcg
      901 gactctttcc gccgacatgc gttacttggg gtggcgacga gatgtttcgc gacccgatcc
      961 gtcgcttcgt cgatcggctc cgcgccgcgg gaatatcgac tgaggcacag gaatttccgg
     1021 gcatgtttca cgtcttccag atcctgatgc cgtgggcaga atccagccgc tcagcattcc
     1081 gcctggtacg agatttcata cacaagatcg tggaggacgc accgctcatc aaggatcctc
     1141 gccaaattca cccgcgcctg gaggcgcagt gaacgaccgt ctcgttggcg cgcttcggac
     1201 aacggattgg ttcgcctgag cgcctagccg aggtgaaggg cgagggcggc agcgcacgtc
     1261 gccgcgacca cgagccccgc gacgacgtcg gagacgccgg gacccggacg ggtcccgaca
     1321 ccggtcgtaa cgccaccacg cgcggtgagg gcctcgccca gctcgccagc tcggcgcatc
     1381 gagaccgcca gggccgcacc cagcagatcg gccggctccg ccaacacgcg ctgggccgtc
     1441 cgtcgcgcct ttgagggatg gcgcgcctga gcatcggcgc cctggacgtc cgcggccacc
     1501 gccccggcga cgtgcggtgg gcggagccga cgcgccgcga gcagggtgcg catctcgtcc
     1561 aa
//
LOCUS       DATAQL010000501         4828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_660253, whole genome shotgun sequence.
ACCESSION   DATAQL010000501 DATAQL010000000
VERSION     DATAQL010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660253"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..636
                     /locus_tag="VHS97_08645"
     CDS             145..636
                     /locus_tag="VHS97_08645"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX3448307.1"
                     /translation="MYHELARELRKNMTDTERLLWSKLRGKQFGGFKFRKQSPIGQFI
                     VDFVCFDRKVVIELDGGQHAISVEDDKKRSEWLKSQGFQVIRFWNHEFIEDSDMVMEA
                     IWLALQRPPTLALPHEGGGNFKGGSYVESPGSGELPSRGPKDSGQIPSGPTKTNSTKD
                     PIE"
     gene            633..2042
                     /locus_tag="VHS97_08650"
     CDS             633..2042
                     /locus_tag="VHS97_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3448308.1"
                     /translation="MTTATSPLPDLIEEAQTTEETFLRQIFVRDQMAEWSKQPIVMAR
                     ADGVYYWDVLGKRYLDAISGIYVVSVGHNNRRIIDAIKKQLDVLAFSPPMHGTNPQAV
                     QLANLLAELAPGDLSTVKFQCGGAEVVEAAIKLARQYHKLTGSPGKYKIISRYQSWHG
                     STLGALSASGLKSRKTVNEPMAPGFIHVFPPTCYRCPFGKSYPDCGITCATIVEDVIA
                     MEDPATVAAILVEPIGHTGGVIDPPEEYLPILRDICDRHNILLIFDEIITGIGRTGRM
                     FAAETFGVKPDVLLIAKGLSGGYAPLSALICRRPIAEAFWGPIATNPGFVEGHTFEGN
                     PISCAAGIAVLSEILERDLLANARKQGERLRSGFETLARKYPVIGDVRGKGLFQAIEF
                     VRDRTTKTRFPDATAFGVQVGRRALAHGLLCRFDPHWIAFGPPLVVTADEVDAMVSIL
                     DLSLGEVWSQLDGAGSAAS"
     gene            2048..3190
                     /locus_tag="VHS97_08655"
     CDS             2048..3190
                     /locus_tag="VHS97_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX3448309.1"
                     /translation="MRRMVPHVRAPVRFLMYARFARYLLWEFRWPLVIFTSLVLLGGL
                     ILHQFYDHGRLPFAHACHAVFLMIFLESSLDFPTEWYLQPLFFLLPVIGLGAVADSVV
                     RLAFLVFTRKQNLPEWNRMLASLCRNHFVVIGVGKVGYQVIRQLLELRESVVAIEMAS
                     GSPLLGELFDKGVPVIQGNARQSSVLEQAGIRQARAVIVTTSDDLTNLDAAITARELN
                     ADAKIVIRLFDETLATKVAGAFSMPTISTSQVAAPAFIAAATGRKIYQGFQLAGQYVH
                     FTDITICPTGRLVGVSVGEIQADKQINIVMHQGAKGVNVNPDADIVLEPGDTILVIAP
                     IEPLLTLESMNQAHPASSHKNARASEANRAERLIGDSLFDKRTPSL"
     gene            3203..4804
                     /locus_tag="VHS97_08660"
     CDS             3203..4804
                     /locus_tag="VHS97_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX3448310.1"
                     /translation="MPESTPKLVAYVSDERYVALPDVILEFEGDAGSFEARSRATGAV
                     HVVMPSGTYRVTLQKPGFGAKRVRVEIASSGPPHHFRLLSDALLGYAWPKWVKAGERA
                     EFRVHSVEPYQLSLWRYGFLKVLVRNLGWFDEHGPRATMQITPDGDYSQTGVAWNKFG
                     YANPALHQYVDAPEQSGLYYFHARTACDAEFGFPWIVAPSKPTSPIAVLASNITWNAY
                     NNFGGRSNYINPDRLPPTPTVNARLELKRYTDPDAVTYDTDDYAPLSFDRPEPINHID
                     LSAVINDPIEGRAAVHIAPAEWRLLGWLEREQFAYDYYAETQLHDGTLDLDAYKVLII
                     STHPEYWSRQMYFAAKSWVHERGGKLMYLGGNGLNCEAEFVDPTTVIYKNGDARAVKG
                     GGYESRFHARVESEAHLLGVVFSETGVMTAAPYRVLKANHWALDGTNLNDGDLFGHSS
                     LHMRCPGGASGHETDKVSPSSPHNVTRLAKGTNVDDGGAELVYYETAKGGAVFSASSI
                     AWPSAILIDDCVSRITVNVLSTFLS"
BASE COUNT          974 a         1471 c         1398 g          985 t
ORIGIN      
        1 tttgtcttac gttacgtaaa agccaattac gactgattca acggtatcgg atctatggac
       61 gaggatagtg ttctgcaact ctaacgcgac aaaatgtttt aactccctcc ccccttgtgg
      121 gggagggctg gggtggaggg cggcatgtac cacgagcttg cacgcgaact gagaaagaat
      181 atgaccgaca ccgagcggct gctctggtcg aagctgcgcg gcaagcaatt cggcggtttc
      241 aagtttcgca agcaatcccc gatcggtcag ttcatcgtcg atttcgtctg ttttgatcga
      301 aaggtcgtga ttgagctcga tggtgggcaa catgcaatct ccgtcgaaga tgacaagaaa
      361 cggagcgaat ggctcaaatc ccagggattc caggtcattc gtttctggaa tcatgagttc
      421 attgaagatt ccgatatggt gatggaagcc atttggctgg cactgcaaag gccccccacc
      481 ctggccctcc cccacgaggg gggagggaat tttaaaggcg ggtcttacgt tgagagtcca
      541 ggcagcggag agcttccctc gcgaggcccg aaggattctg gccaaatccc atccgggccg
      601 acgaaaacga actccaccaa ggaccccatc gaatgacgac cgcgaccagc cctttgcccg
      661 acctgatcga agaggcccag acaacggagg aaacgttcct ccggcaaatc ttcgtgcgcg
      721 accagatggc cgagtggtcg aagcaaccga tcgtgatggc gcgcgccgac ggcgtgtatt
      781 actgggacgt tctgggcaag cgctatctcg acgccatctc gggcatctat gtcgtttcgg
      841 tagggcacaa taaccgccgg atcatcgacg caatcaaaaa gcagctcgac gtgttggcct
      901 tttcgccgcc aatgcacggc accaacccgc aggccgtgca attggctaat ttgctggccg
      961 agctggcgcc aggagacctc tcgacggtca agtttcaatg tggcggagca gaggtcgtcg
     1021 aagcggccat caagctggct cggcagtacc acaagctgac aggatcgcct ggcaagtaca
     1081 agatcatcag ccgctaccag tcgtggcatg gctccacatt gggagccctg tcggcctcgg
     1141 gcctcaagtc gcgcaagacg gtcaacgaac ccatggcgcc ggggttcatc cacgttttcc
     1201 cgccgacgtg ctaccggtgt ccgttcggca agagctaccc cgactgcggc atcacctgcg
     1261 cgaccatcgt cgaggatgtg atcgccatgg aagaccctgc aacggtggcg gccatacttg
     1321 tcgagccgat cggacatacc ggcggcgtga tcgatcctcc cgaagaatac ctgccaattc
     1381 tccgcgacat ctgcgaccgg cataacattc tcttgatttt tgacgagatc atcactggca
     1441 tcggtcgcac gggcaggatg tttgccgcgg agacttttgg cgtcaagccc gacgtcctct
     1501 tgatcgccaa aggcttaagc ggcggctatg cacctttgtc ggcattgata tgccggcgac
     1561 ccattgccga agccttctgg ggcccgatcg cgaccaaccc ggggtttgtc gagggacaca
     1621 catttgaagg caatccgatc tcgtgcgcgg ccggaatcgc cgtgctgagc gagattctcg
     1681 aacgcgacct gctggccaac gcccgcaaac aaggagagcg gctgcgctct gggttcgaaa
     1741 cactggcgcg gaagtatccc gtgatcggcg acgttcgcgg taaggggctc tttcaggcca
     1801 tcgaattcgt gcgcgaccgt acgacgaaga cacgttttcc cgacgcgacg gcattcggag
     1861 tccaggtcgg gcgacgtgcg cttgcccatg gactgttgtg ccggttcgac ccacactgga
     1921 tcgccttcgg accgccgctg gtcgttacgg ccgacgaggt cgacgcgatg gtctcgatcc
     1981 tcgacctcag tttgggcgaa gtttggagcc agttggacgg cgcggggagc gctgcgagct
     2041 aaccgccatg agacgaatgg ttcctcacgt ccgcgcgccg gtgcggttct tgatgtatgc
     2101 ccggttcgca cgctacctgc tctgggagtt ccgctggcct ttggtcatct tcacgagcct
     2161 ggtgctcttg ggaggcttaa tcctccacca gttttacgac cacgggcggc tgccgtttgc
     2221 gcatgcctgc cacgctgtgt ttctgatgat ctttctcgag tcgtcgctcg acttccccac
     2281 agagtggtat cttcagcctc tattcttcct gttgccggtt ataggcctcg gagcggttgc
     2341 cgactcagta gttcggctcg ccttcctggt ctttacgcgc aagcaaaact tgccggagtg
     2401 gaatcgcatg ctcgcctcgc tctgccgcaa ccatttcgtc gtgatcgggg tcggcaaggt
     2461 cggctatcag gtcatcagac aactcctcga actgcgcgaa agtgtcgtgg cgatcgagat
     2521 ggccagcggc tcgccgttat tgggcgaact ttttgacaag ggcgtgccgg tgatccaggg
     2581 aaacgcccga caatcgtcag tcctggaaca ggcgggcata cggcaggcga gggccgtcat
     2641 cgtcacaacc agcgacgacc tgacgaacct tgacgccgcc atcaccgccc gcgagttgaa
     2701 cgccgacgcc aagatcgtga ttcgactttt tgatgagact ttggcgacaa aagtcgccgg
     2761 cgccttttcg atgccaacga tctcgacgtc gcaggtggca gcccctgcgt tcatcgcggc
     2821 ggcaaccggg cggaagatct accaaggctt tcagctcgca ggccagtacg tccactttac
     2881 ggacatcacg atctgcccga ccggccggtt ggtcggagtg agcgtcggcg agatccaggc
     2941 cgacaagcag atcaatatcg tcatgcacca aggcgccaag ggtgtgaacg tcaaccctga
     3001 cgcggacatc gtgttggaac cgggcgacac gatcctggtg atcgcgccca tcgagccctt
     3061 gctgacgctc gagtcgatga accaggctca tccagcctcg agccataaaa acgcgagagc
     3121 cagcgaggca aatcgagctg agcgattaat tggtgactcg ctctttgaca agcggactcc
     3181 ctcgctttag agtacccgct ccatgccgga atcgactccg aagctggtag cctacgtcag
     3241 cgacgagcga tatgtcgcct tgcccgacgt catcctcgag tttgagggag acgccggttc
     3301 gttcgaggcc cgctcgcgtg ccaccggcgc cgttcacgtt gtgatgcctt cggggacgta
     3361 cagagtcacg cttcaaaagc ccggcttcgg ggccaagaga gtgcgagtcg agatcgcttc
     3421 ttctggcccg ccccatcatt tccgtctcct atcggacgcc ctgctcggct acgcctggcc
     3481 aaaatgggtc aaggccggcg aaagggccga gttccgcgtg cactccgtcg agccttatca
     3541 attgagtctc tggcggtacg gtttcttgaa ggtgctggtc cgtaacctcg gttggttcga
     3601 cgagcacggc ccgcgggcca cgatgcaaat tacgcccgac ggcgattatt cccagaccgg
     3661 tgtcgcctgg aacaaatttg gatacgccaa ccccgcgctg caccaatatg tcgatgcccc
     3721 cgagcagtct ggcctttact attttcatgc gcgcacagcc tgtgacgccg agtttggctt
     3781 tccctggatc gtcgcgccct caaaaccaac gtcgcccatc gccgtgctcg catcgaacat
     3841 cacctggaat gcctacaata atttcggcgg ccgcagcaac tacataaacc ccgatcgcct
     3901 gccgccgact cccacggtca acgcccggct cgagctcaag cgctacaccg atccggacgc
     3961 ggtcacatac gataccgacg actacgcacc gttatccttc gaccgaccgg agccgatcaa
     4021 tcacatcgat ctgtccgccg ttatcaacga cccgatcgag ggccgggccg ccgtccacat
     4081 tgcgcctgcc gagtggcggc tactcggttg gctcgaacgc gaacagttcg cctacgacta
     4141 ttatgccgaa acccagcttc acgacggcac gctcgacctc gatgcgtaca aagtcttgat
     4201 catttcaacg catccggaat actggtcccg gcagatgtat ttcgcggcca aatcgtgggt
     4261 tcacgagcgc ggaggcaagc tcatgtacct gggtgggaac ggactcaact gcgaggccga
     4321 gttcgtcgat ccaaccactg tgatctacaa gaacggcgac gcccgtgccg tcaagggcgg
     4381 aggctacgag agccggtttc atgcgcgagt ggagtcagag gcccacttac tcggcgtggt
     4441 gttttccgag accggcgtca tgactgctgc cccgtatcgt gtcttgaagg ccaaccattg
     4501 ggccctcgac gggacgaacc ttaatgacgg cgacctcttc ggccactcca gcctgcatat
     4561 gcgctgtccg ggcggggcct cgggccatga aaccgacaag gtctcgccca gctcgccgca
     4621 taacgtcact cgcctcgcaa aagggaccaa cgtcgacgat ggcggcgccg aactggttta
     4681 ttacgagaca gccaagggcg gcgctgtctt ttcggccagc tcgatcgcgt ggcccagcgc
     4741 gatcttgatc gacgattgtg tttcgcggat cacggtaaat gtacttagca cgtttctctc
     4801 atagcttatt ctgttgacag aaaacgta
//
LOCUS       DATAQL010000502         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2645437, whole genome shotgun sequence.
ACCESSION   DATAQL010000502 DATAQL010000000
VERSION     DATAQL010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2645437"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /gene="thiL"
                     /locus_tag="VHS97_08665"
     CDS             complement(<1..857)
                     /gene="thiL"
                     /locus_tag="VHS97_08665"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX3448311.1"
                     /translation="MRNVSLWPSLEFYTRAQIVRNRMNNHHRTFGEFDLIDWIRQREG
                     SQGCPWTKLGIGDDCAILDVNRAGDLLVTTDMLMDGRHFRLAHDGPRAAGYKALGVNL
                     SDIAAMAGVPRACVVAVALPRTEAATIAQEIYAGMHELATRFGVDLVGGDTNAWDGPL
                     VISVTLLGETTPRGAIRRAGAQPGDIILVSGPLGGSLFAGRHLRPEPRIALALATHEA
                     VPIHALIDISDGLSSDLGHILEESGGLGAILDEAAIPIHSDAHEMSQCDGTPALDHAL
                     NDGEDFELCL"
     gene            complement(857..>1813)
                     /locus_tag="VHS97_08670"
     CDS             complement(857..>1813)
                     /locus_tag="VHS97_08670"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX3448312.1"
                     /translation="VTGPLGKPVQAFLGLMGDGQSAVIEMAASSGLPLVPTEQRDPRI
                     ATTYGVGELILAALTEGRTHFIIGIGGSATNDGGAGFGQAIGFRLLDDQDRDLEPGGG
                     NLARLARIDRLARRSELEGVEIAVACDVTNTLCGPRGASAVYGPQKGANPSMVEILDR
                     NLAHFAAVVERDLGAAIKDLPGSGAAGGLGGGLVAFAGGKLEPGVELIIDAVKLAERL
                     EKADLCLTGEGAIDAQSAFGKTAVGVAQLAQSLHCPTLALAGSIGPGARAVLEHGIDA
                     YFAICPGPLSLEQAIDQASLLLEQSAEQAVRGFLAGQHRRSF"
BASE COUNT          322 a          585 c          597 g          309 t
ORIGIN      
        1 aggcaaagct cgaagtcctc accgtcgtta agggcgtgat cgagtgcggg cgtgccatcg
       61 cattgactca tctcgtgagc atcagaatgg atgggaatcg ccgcctcgtc gagtatggca
      121 cccagtccgc cactttcctc aagaatgtgg cccaggtcgg acgacagccc gtcggagatg
      181 tcgatcaggg cgtggatcgg gacggcttcg tgggtggcca gagccagggc gatccgcggc
      241 tcggggcgga ggtgacggcc ggcaaacagg cttccgccca gtggaccgct tacgagaatt
      301 atgtccccgg gttgggcgcc ggcgcggcgg atcgccccgc gcggcgttgt ctctcccagt
      361 agagtgacgc tgattacgag ggggccgtcc cacgcattgg tgtcgccgcc gactaagtcg
      421 acaccaaacc gcgtggccag ctcatgcata ccggcataga tctcttgtgc aatggtggcc
      481 gcctcggttc gcggcaaggc gacggcaacc acgcaggcgc gcggaacgcc cgccatcgcc
      541 gcgatgtcgg acaggttgac acccagcgcc ttgtagccgg cggcgcgcgg gccgtcgtgg
      601 gccagccgga aatggcggcc gtccatgagc atatcggtcg tgaccagcag gtcacccgcg
      661 cgattgacgt cgaggatcgc gcagtcgtcg ccaatgccca gcttggtcca agggcacccc
      721 tgcgacccct cgcgctggcg gatccaatcg atcagatcga attcgccgaa agttcgatga
      781 tgattgttca tgcgattgcg gactatttgg gctcgggtgt agaactccag cgatggccaa
      841 agagaaacat tgcgcatcag aaactgcgcc ggtgctgacc cgccagaaac cccctgacag
      901 cctgttcggc cgactgttcg agcaataggc tggcctggtc gatcgcctgc tccaggctca
      961 gggggccggg gcaaatcgca aaataggcgt cgattccatg ttcgagaact gccctggcgc
     1021 ctggaccgat agagccggcc agggccaggg tgggacagtg aagcgattgc gctaactggg
     1081 cgacaccgac ggccgtcttg ccaaaggcgc tctgggcatc gatcgcccct tcgccggtca
     1141 agcagaggtc ggccttttcg agtcgctctg ccagcttcac ggcgtcgatg atcagctcga
     1201 cgccgggctc gagcttgccg ccggcaaagg cgaccaggcc gccccccagc ccgccggcgg
     1261 cgccggagcc gggtaagtct ttgattgccg caccgaggtc gcgttccacg acggccgcga
     1321 aatgcgccag gtttcggtcg agtatttcga ccatcgacgg gttagcaccc ttttgcggac
     1381 catagacggc ggaggctccg cgcgggccgc acagggtgtt ggtgacgtcg caggccacgg
     1441 cgatctcgac gccttcgagc tcactgcggc gcgccaatcg atcgatgcgc gccaggcgag
     1501 ccaggttgcc accgccgggt tcgaggtcac gatcttgatc gtcgagcaac cggaagccga
     1561 tggcctgtcc gaatccggct ccgccatcgt tggtcgcgct gccgccgatg ccgataataa
     1621 agtgcgtgcg gccctcagtg agcgcggcca ggatcagctc gcctactcca taggtcgtgg
     1681 caatgcgggg atcgcgctgc tcagtgggca cgaggggcag acctgagctg gccgccatct
     1741 cgatcacggc gctctgccca tcgcccatca gcccaagaaa cgcctgcacc ggttttccca
     1801 gcggccccgt cac
//
LOCUS       DATAQL010000503         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_660741, whole genome shotgun sequence.
ACCESSION   DATAQL010000503 DATAQL010000000
VERSION     DATAQL010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660741"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..766
                     /locus_tag="VHS97_08675"
     CDS             158..766
                     /locus_tag="VHS97_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3448313.1"
                     /translation="MANQNSVTIWIDGIKAGDGSDIERLWDRYFERLVRLAAARLPAH
                     SRRSFDEEDVALSAFQSFCNRAGRGQFPQLSDRDDLWRLLATITVRKALDTIRQQSRQ
                     KRGGGQVLGESALVVGAGTEGDGLAEVLSREPTPNEVAGFAEDYHRFLARLQEPPLRT
                     IALRRLEGQTTREIARALDVSTKTVERKLQLIRAIWSEESSR"
     gene            complement(936..1667)
                     /locus_tag="VHS97_08680"
     CDS             complement(936..1667)
                     /locus_tag="VHS97_08680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3448314.1"
                     /translation="MELPGITICWPGAKWPILNVIGLSGPVTDQADLDAKVQQAVSYT
                     QTKPVMGMVVTCTEWLPPDLEERNVAKFAEHGFALGLALRGMVADQLLSPRRPLPDLE
                     YRRVSSLETSRACVDLNAISYDAPLEFGREMSDITGFWDGVFAYVGYRNGKPVATAAT
                     FLECGRLYVGFVATLPDARSNGYAEAVVRHSLEAAARSSGFSRTVLHATELGYPVYRA
                     MGYRTVASFRAFLPQSIVNGTSNPA"
     gene            complement(1826..2653)
                     /locus_tag="VHS97_08685"
     CDS             complement(1826..2653)
                     /locus_tag="VHS97_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3448315.1"
                     /translation="MRTTAETKYILGHSGREIQRLMHQAAIVRPITERLLRSAGIGQG
                     MRVLDLGCGAGDVSLLAAELVGPSGSVVGIDRNQAIIAMARQRARRAKFHHVDFRYVS
                     VEDFSDPEPFDAVVGRFVLIHQADPVALIRAAADFARPGGVVAFHEPYIQVHSFQSLP
                     IVSLWQQAGEWLWMAFQEPAPHFDAGGRLVEHFSRAGLRQPSLFCECIVGGGKDSPLY
                     AWMADILASVMPQLGRMGILTEDLIAIETFEDRLRTAVVEANSQIVGPPQFCAWARV"
     gene            3452..4033
                     /locus_tag="VHS97_08690"
     CDS             3452..4033
                     /locus_tag="VHS97_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3448316.1"
                     /translation="MMGTKPQQDLTAILGRARAGDQRAKGELIALVYDELRQVAARLM
                     RRERTGHTLSTTSVVHEAVIRLLDKAVFDKAADRNYLFASAARAMRQVLVDHARRRSA
                     ERRGGRRRRVPLDVVVDELEEQKLDVVAVHEALDRLAEWNGRLAQVMTLRYFGQLTVP
                     EVAAALAISPATVERDWRLARAWLRGHLRGEDE"
     gene            4030..>4881
                     /locus_tag="VHS97_08695"
     CDS             4030..>4881
                     /locus_tag="VHS97_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX3448317.1"
                     /translation="MTPEQWSRIEKLFAHGLEIDPAERQVWLLAACGDDEELRAEVAR
                     LLDQDGRAARDRFLTASAPPRLDPDQTGSWSSHSGHAPPRGQAQFVDSSSDFVAGIHG
                     FSPRPAIATGPKPHPITDISVVRARLRELPMIHILILAMANCWRYMILGDNDPVLRYL
                     DLSILALLATAIALLWSRWRFSLAWLQALELAMIGILAMRLAIIEYRQILSFSLWHDS
                     AMAQFIMKNIVLLDAVLIASYGIYVPKNWRHAALVVGPLALLPFATLSILYLQHPDAM
                     AWLGQGAM"
BASE COUNT          933 a         1499 c         1492 g          957 t
ORIGIN      
        1 tccgaaaata agaaattggg ttgtaaatct cggagtgcgc gaccgcccga cgatggcgcg
       61 ttgtcgcggc cggcatgtcg ttgcaccatt tgcggcgatg gatcacgcga gccggttacc
      121 ggtttcttgc cgctctatcg gctgagaggt tttggccgtg gcgaaccaga attcagttac
      181 gatatggatt gatggaatca aggcgggcga tgggtcggac atagagaggc tgtgggatcg
      241 gtatttcgag cggttggtcc ggctggccgc tgcgcgactg ccggcacatt cccgtcggtc
      301 gttcgacgag gaagatgtgg ccttgagcgc ctttcagagt ttctgtaatc gcgccggtag
      361 gggccagttc ccgcagctga gtgatcgcga tgacctctgg cggctcttgg ccacgatcac
      421 ggtgcgtaaa gcgctcgata cgattcggca gcagagccgc cagaaacggg gcggaggcca
      481 ggtgctgggt gaatcggccc tggttgtggg cgcgggcact gaaggcgatg gcctggccga
      541 agtccttagc cgagagccga ccccgaacga ggtcgccggg tttgccgaag attaccaccg
      601 atttctcgcc cggctccagg agccccccct gcgcaccatc gccctacgga ggctagaggg
      661 ccagaccacc cgcgagatcg cccgcgcgct ggacgtctcg acaaagaccg tcgagcggaa
      721 actccaactg atccgcgcga tttggagcga ggagagttcg agatgaacgc gcgatgggaa
      781 acgcccgtac gctgtccctg accacggcat tcgtctcccg tccgagccag tcattcggaa
      841 tcccagagac caacgtccag gccgcctcgt gtgccggttc accacgtgtt actcgatcca
      901 acgacccgcc gtgggtggcc gcgtcttttt ggtcatcatg cgggattgct cgtcccattg
      961 acgatcgatt gaggaaggaa agcacggaaa ctagcgaccg tccggtatcc catcgctcgg
     1021 tagacggggt aaccgagttc ggtggcgtgg aggacggttc gcgagaagcc gctgcttcgg
     1081 gctgcggctt cgagagaatg gcgcacgact gcttcggcgt aaccgttgct gcgcgcatcg
     1141 ggcagggtag ctacgaagcc gacgtaaagg cgtccgcatt caagaaaggt cgccgcagta
     1201 gccacgggtt tcccgtttcg atagccgacg taagcgaaaa caccatccca aaaaccggtg
     1261 atgtcagaca tctctcggcc aaattccagg ggcgcgtcgt agctgatggc attcaaatca
     1321 acacaagccc tgctggtttc gagcgaggat acgcgtcggt attccaaatc cggcagcggc
     1381 cgccgcgggg acaggagttg gtctgcaacc atcccgcgta gcgctaagcc gagtgcgaat
     1441 ccgtgctccg cgaatttcgc tacgttcctc tcctcgagat ccgggggaag ccactcggtg
     1501 caggtgacga ccattcccat gaccggtttg gtctgggtgt agctgacggc ttgctggact
     1561 ttcgcgtcca aatccgcctg gtcagtgacg ggacccgaca atccgattac atttagaatg
     1621 ggccattttg cgcccggcca acatatcgtt atgccgggca attccacggt ctcgtggttc
     1681 gcgaatttgc ggcccagctt gttccacact ccgtagaatt gggcgttcga ctcgtcgatc
     1741 tccgttggaa tggtcagggc cacgctcaaa cctccacgat ctacgttcgc cctctctgaa
     1801 cactcacgcg ccgtctcatc cgcttctaga ctctggccca agcacagaat tgcggcgggc
     1861 cgacgatctg actatttgcc tcgaccacgg ccgtccggag ccgatcctca aacgtctcga
     1921 tggcgatcaa gtcctctgtg aggattccca ttcggccgag ctgcggcatg acgcttgcga
     1981 ggatgtccgc catccaggca taaagaggcg aatccttgcc gccaccgacg atacactcac
     2041 aaaacaagct cggttgtcgc aggcccgcgc gggaaaagtg ctcgaccaac cggccgcccg
     2101 cgtcaaagtg cggggccggc tcttggaacg ccatccagag ccactctccg gcctgctgcc
     2161 agagcgagac gataggcagc gactgaaaac tatgtacctg aatatatggt tcgtggaagg
     2221 ccacgacccc tcctggtcga gcaaaatccg cggcggcgcg gattagggcg acgggatcgg
     2281 cctgatgaat caagacaaat cggccgacca ccgcatcgaa tggctcgggg tcggaaaaat
     2341 cctcaaccga cacgtatctg aagtcgacgt gatgaaactt ggccctccga gcccgttgtc
     2401 tggccatggc gatgatggct tggttgcgat caatccccac gaccgatccc gatggaccga
     2461 ccagctcagc agcgagcagc gagacgtcgc cggcaccgca gcccaggtcg agaacccgca
     2521 tgccctggcc gatccccgca ctccgaagga gccgctcggt gatcggccgc acgatcgccg
     2581 cctggtgcat cagccgctgg atctctcgtc ctgaatgtcc taggatgtac ttagtttcag
     2641 ccgtggtgcg catttccgct ccccgtttat cgttgcgggc acgcggcgtg cgcagtgata
     2701 ccgtcgcgcc cgcgactcgt aacttctttg ctccattggc cctgcgccct cgggccactc
     2761 agctcagacg gaccgaccaa gagctccgct cccggccact tcgacagctc ctgcgaataa
     2821 tgccgccgga acccatcacg ttttttcttg tccactctat tacgcgcaag aacgggcgct
     2881 catcgtcacg agttgagggg aggattagcg tcttaccatt cagacagttt tgattgcaaa
     2941 gaggcgtgtc gtgccgatcg catgcgggga ctcggcgggg aattaaggtc aggaaaccaa
     3001 cggggtgtcg cagtcacgat tgttttgaca atgtactgta tagatcgaga ccgaacgggt
     3061 cggacgggag ttgacggtca cgcatgtccg taccgagtcc gtcggtctgc ggggacgctt
     3121 tttaccgggc tctgctgttc gtgatggacg aatctacacc gagcggttcg gatccccaat
     3181 cggggcgcgg tcgtctagaa tgacccagct ctccccatgc gactcgccgc ctcgggctgc
     3241 cgtggaactt ctggacggat catccacgtc cctttcgagg cgcggaatgt aaagcgcttt
     3301 cgacacatcg cccggtcata tttccaaaac atctcactcg cgatccgcga gttcgcgttc
     3361 ggcaaataca taagattagg tgtcagcccg ccgtggcggt gcctcatgag gccatcggca
     3421 cgattgcggg ccgcaggaaa gccatggtga catgatggga acaaaaccgc agcaggatct
     3481 gacggccatt ttgggacgag cccgagccgg ggaccagcgg gccaaaggcg agctgatcgc
     3541 gcttgtatac gacgagctgc gccaagtcgc cgcgcggttg atgcgccggg aacggaccgg
     3601 ccataccctc tcgactacct ccgttgtcca cgaagccgtg attcggttgc tggacaaggc
     3661 ggtcttcgac aaggcggcgg accggaacta cctgtttgct tccgccgccc gggccatgcg
     3721 gcaggtctta gtcgaccacg cgaggcgtcg gtccgccgag cgacgcggag gtcgccggcg
     3781 gcgcgttccg ctggacgtgg tcgtggatga gctcgaggag caaaaactcg acgttgtcgc
     3841 cgttcacgag gccctcgatc ggctggccga gtggaacggg cgcctggccc aggtgatgac
     3901 cctgaggtac ttcggccagt taactgtccc tgaggtcgcc gcagcgctcg cgatctcgcc
     3961 agccacggtc gagagggact ggaggctggc gcgggcctgg ctccgcggtc atctccgagg
     4021 agaggacgaa tgacaccgga acaatggagc cgcatcgaaa aactattcgc tcatgggctc
     4081 gagatcgacc ccgcagagcg acaagtgtgg ctcctcgcgg cttgcggcga cgacgaggag
     4141 cttcgggcag aggtcgcccg ccttctggac caagatggga gggcagcccg cgaccggttc
     4201 ctgacggcgt ctgcgccgcc gcgcctggat ccggaccaga ccggaagctg gagctcccac
     4261 agcggacacg ctcctccacg tgggcaggcg caattcgtcg attcttcgag cgattttgtc
     4321 gccggcattc acggcttttc tccccggcca gcgatcgcca ccggcccgaa gccgcacccg
     4381 atcaccgaca tatcggtggt gcgggcgcgg ctgcgcgaat tgccgatgat ccacatcctc
     4441 atattggcga tggccaactg ctggaggtac atgatcctcg gggacaacga ccctgttctt
     4501 cgttatctcg atctatccat cctggcgctc cttgcgaccg ccatcgcctt actctggagt
     4561 cgttggcgat tctcgctcgc ctggctccag gctctggagc tggcaatgat cggtattttg
     4621 gctatgcggc tcgcgatcat cgagtatcgc cagatcctga gcttctcact atggcacgat
     4681 tcggcgatgg cccaatttat tatgaagaat atcgtactcc tcgacgccgt cctgatcgcc
     4741 agctatggta tctacgtccc caaaaattgg cgacatgccg cgcttgtggt gggaccgctc
     4801 gcgctcttac ccttcgcaac attgtcgatc ctctaccttc agcaccccga cgcgatggcc
     4861 tggctggggc aaggggcgat g
//
LOCUS       DATAQL010000504         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2491808, whole genome shotgun sequence.
ACCESSION   DATAQL010000504 DATAQL010000000
VERSION     DATAQL010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491808"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1834
                     /locus_tag="VHS97_08700"
     CDS             95..1834
                     /locus_tag="VHS97_08700"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3448318.1"
                     /translation="MIGRSMRVVVGLCSIAVVGSAPAQEAAVAPGRRVDAPAVAGRTE
                     WQAAGTQGAVAAGGRDAVAAGLEILKKGGNAADAGAATILALSVTDSRAFCFGGEVPI
                     MIYDAQRRGITVIAGQGAAPRLATLDHFKGPGGIPASGIEAAAVPAALDACLTLLDRF
                     GTMTFAQVIAPAQALLRKPARPQPWHADLARTLETLIEAEAQAYPASSASAHGDRARG
                     LRLVADAFYRGPIARRIDAWSRAHGGLIRYVDLATHTTRIEDPVAVNYRGQTVYKCGA
                     WTQGPYLLEALQILKDVELKAMGHNRPATVHTLVETLKLAMADRDTFYADPLFADVPL
                     SALLSPEYAALRRGLIDPQRASLVRRPGDPLGHKAVLDPAADPAQRHVLPPPSPAHDT
                     TTCVVADRFGNVVAATPSGWTGVVAGDTGVWLGSRLQSFNVWEDHPNVIVPGKLPRVT
                     LSPTLVLRDNRPVLAISVAGGDNQDQMTLQLLVNHFDFGLDPAESVIAPRFMTDHFIS
                     SFRQTPPALGQLRINPEIGSGTLDALKALGHGLSNRTGPLAAAPSVIALDPASGQIRA
                     AGDPRAGRHSLAY"
     gene            complement(1900..>2309)
                     /locus_tag="VHS97_08705"
     CDS             complement(1900..>2309)
                     /locus_tag="VHS97_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448319.1"
                     /translation="IGLARIALEQGRLEDAQARIFGLERRGFLEPEAEKIKAELTLRL
                     QAGGVGSVESARAALAAKPDDPNLEFQLAEALAAAGQYEEALAVCLDLVERDRKGIGE
                     KARQTMVAIFQLLPPDSELAIEYQRQLSLALME"
BASE COUNT          362 a          790 c          758 g          399 t
ORIGIN      
        1 tcggcctcga aggcactaaa ttcaacgctc tattttccag tgcgacaaaa cgcgattggt
       61 tggctttcat ttttcccggc gggaggtggg ggcgatgatc ggacgctcga tgagggttgt
      121 ggtgggactt tgctcgatcg ccgtcgtcgg cagtgcaccg gctcaggagg cagccgttgc
      181 gccaggccgt cgggtggatg cgccggccgt cgccggccgc accgagtggc aggccgccgg
      241 aacccaaggg gccgtcgccg ccggcgggcg cgacgcggtt gcggcgggac tggagatctt
      301 gaaaaagggg ggaaatgccg cggacgcagg ggctgcgacg atcctggctc tcagcgtgac
      361 cgactcgcgg gcgttctgtt tcggtggtga agtgccgatc atgatttatg acgcccagcg
      421 acgcgggatc acggtgattg ccggccaggg ggccgcgcct cggctggcga cgctcgatca
      481 tttcaagggc cccggcggga tccccgcaag cgggatcgag gccgcggccg tgccggccgc
      541 gctcgacgcc tgcctcacac tgctcgaccg gtttggcacg atgactttcg cccaggtgat
      601 cgcgcccgcc caggccctgc tccgcaagcc cgcaaggcct cagccgtggc atgccgatct
      661 cgcgcggact ctcgaaacgt tgatcgaagc cgaggcgcaa gcctaccccg caagctcggc
      721 ctccgcccat ggcgatcgtg ctcgtgggct gcgcctcgtc gccgacgcgt tttatcgcgg
      781 accgatcgcc cgccggatcg atgcctggtc ccgcgcccac ggcggcttga tccgctatgt
      841 cgatctggcc acccacacga cgcggatcga agaccccgtc gccgtgaact atcgcggcca
      901 gaccgtctac aaatgcggcg cgtggactca aggcccttac ctgctcgagg cgctgcagat
      961 tctcaaggac gtcgaattga aggcgatggg gcataatcga cctgcgaccg tccatacctt
     1021 ggtcgagacc ctcaagctgg ccatggccga tcgcgacacc ttctacgccg acccgctctt
     1081 cgccgacgtg ccgttgtcgg cgctcctctc acccgagtac gccgcgttga ggcgggggct
     1141 gatcgaccca cagcgcgctt cactcgtgcg ccggccgggc gatccgctgg ggcacaaggc
     1201 ggtgcttgac ccagcggctg acccggccca gcggcacgta cttccgcccc cctcccccgc
     1261 gcacgacacg acgacctgcg tggttgccga ccggtttggc aacgtcgtgg ctgcaacgcc
     1321 cagcgggtgg accggtgtcg tcgccggcga cacgggcgtc tggctaggca gccggctcca
     1381 gagtttcaac gtgtgggagg atcacccgaa tgtcatcgtg ccgggcaagc taccgcgggt
     1441 caccttgtcc ccgacgctcg tgctgcgcga caaccggccc gtcttggcca tcagcgtcgc
     1501 cgggggcgat aatcaagacc agatgacctt gcagctcttg gtcaaccatt tcgacttcgg
     1561 actcgatccc gccgaatcgg tcatcgcccc tcggttcatg accgaccatt tcatcagctc
     1621 gttccgccag acgccgccgg ccctggggca gttgcggatc aatcccgaga tcggttcggg
     1681 cacgctcgac gccttgaaag ccctcggaca cggattgagt aacagaactg gcccgctggc
     1741 tgccgcgccc agcgtgatcg cgctggaccc cgctagtggt caaatccggg cggccggcga
     1801 cccgcgggcc ggccggcact cgctggcgta ctgatcatgc aactcgccga atgtcgccgg
     1861 ccgagtcggt ggcacacggc caaaccccgc ctttacccgt cattccatga gtgccagcga
     1921 tagctggcgc tgatattcga tggcgagctc ggaatcgggg ggcaggagct gaaagatcgc
     1981 aaccattgtc tggcgggcct tttcgccgat tcccttgcgg tcgcgctcga caaggtcgag
     2041 gcaaacggcc aaggcttcct cgtactggcc ggcggccgcc agggcctcag cgagctggaa
     2101 ctctaagttt gggtcatccg gcttggcggc cagggccgcg cgtgcggact cgacgctgcc
     2161 gacgccacca gcctggaggc gcagcgtcag ctcggccttg attttctctg cttcgggctc
     2221 gagaaacccg cgacgctcga ggccgaagat cctggcctgg gcgtcctcga ggcgcccctg
     2281 ctcgagcgcg atccgcgcga ggccgatct
//
LOCUS       DATAQL010000505         3885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4172444, whole genome shotgun sequence.
ACCESSION   DATAQL010000505 DATAQL010000000
VERSION     DATAQL010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4172444"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..601)
                     /gene="rbfA"
                     /locus_tag="VHS97_08710"
     CDS             complement(155..601)
                     /gene="rbfA"
                     /locus_tag="VHS97_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX3448320.1"
                     /translation="MSTHRSLRMAEAIREVVATAILFEVADPRVNSVTVLSVDVSGDL
                     RNATVNVSIMGNETQRRQAYRGLVHATGFLQARVAARLQTRFTPVLVFKLDDGVKKSV
                     ELARLIDDAVASDRKPENSLSEPIGATPVESIVPPSTHKHAHGDTK"
     gene            complement(745..2769)
                     /gene="infB"
                     /locus_tag="VHS97_08715"
     CDS             complement(745..2769)
                     /gene="infB"
                     /locus_tag="VHS97_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX3448321.1"
                     /translation="MPAPLGPTMTAAEEEEEKKKTGRLGSAADRAGRRARRSERAVDR
                     RVTSPVPAAALLSDDEDTRRTRSGTKKSRGGQRIAIAPSRKSHAEIEAPINIRSLSLA
                     IGVKANDLIRKLMAMNQLATINASLDDDVATMLALEFGIELEVVHMRTAEDDLLDSFE
                     SSGSSENLQLRPPVITILGHVDHGKTSLLDRIRKSHVVDSESGGITQHIGAYQVESNG
                     RPITFVDTPGHEAFTSMRARGANVTDIVVLVVAADDGVMPQTVEAIAHAKAAAVPIVV
                     ALNKIDLPNVDTPSNINKIYGELSTQGLNPVEWGGDTEVVKTSAASGLGITDLLATLE
                     TIAELHELKADPTRPATGTCLEASLSEGRGVLASLLVQDGTLKVGDVIVCGDGFGRVR
                     AIFNDKGRSIETAGPSTPVEISGLDIVPSAGENFGVIDDITSARDIAETRRTRARDAA
                     HADRQSVTLENLYNKMAEQKLKSLNLILKADVQGSIEALTKELEKLENAEVPIRVLLK
                     AVGGISESDIVLADASQAIVIGFRVAPEDRAITLADEKKIEIRRYDIIYQVTDDIKKA
                     VEGMLIPEIKEVHLGRAVVRQVFKISKVGAVAGCFVTQGTIERSAKVRLIREGREIYK
                     GSIEALKRFKDDVKEVPQNFECGIKIGNFDDIKSDDVIEAYRIDIIRRTL"
     gene            3227..>3885
                     /locus_tag="VHS97_08720"
     CDS             3227..>3885
                     /locus_tag="VHS97_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448322.1"
                     /translation="MSGWKPDAPAVGDDDPVEGWMPLGSCRGPPPPGGGPPGRGPVPR
                     CGVWAESGPAEGLVPEKPPGRASGAALNPDGGAVAGRGAPELGGDAAIPTGEGGFADA
                     EGDEPGINRCGGAVAGTGVLDAVGAGSAARTGSILAEPAATGAGPTGRAGGAGRSSPA
                     LARGATVADAANDDGAAAGLAAEAAVADPLRAAGWSINSRIRSTMSGSRLARAFALTS
                     RP"
BASE COUNT          651 a         1170 c         1284 g          780 t
ORIGIN      
        1 cgaaagagcc tggttggcat gttcgcgcgt cgtatcttcg tgacatatcc cataaacgac
       61 cgccttcaga accgaaagcc gcgacgcgct acgatcggcc ttgggtttat agcggcgctt
      121 gagcattgta tgaacatttg agagcaactg cgttttactt tgtgtcgcca tgggcatgtt
      181 tgtgggttga aggcggaacg atcgactcta ccggagtcgc gccgatcggt tcactcaggg
      241 aattctccgg cttccggtcc gaggcgacgg catcatcgat tagacgcgcc agttcgacag
      301 attttttgac accgtcatcg agcttgaaca ccaagaccgg cgtgaaacgg gtttgcagcc
      361 gcgctgccac gcgagcttgg aggaagcccg tcgcatgaac gagtcccctg taggcctgcc
      421 tccgctgggt ttcgtttccc atgatcgaca cgttgaccgt ggcattgcgc aagtcgcccg
      481 aaacgtcgac cgatagcacc gtgaccgagt tgacacgggg atccgcgact tcgaacaaga
      541 tggccgttgc aaccacctcg cgaattgctt cggccatccg caggctgcgg tgtgtcgaca
      601 ttgcttcaga cctcggtcga tgcttgacgg tcaagatgag ctttggatgc tcatctgttg
      661 accgtcccat tgaacttcac acgtgggttt cagggtgtcg ttacatgaga atggccactc
      721 atattcgagc gtccgttcca gcgattagag cgtgcgccga atgatatcga ttctgtaggc
      781 ttcgatgaca tcgtcggact tgatgtcgtc gaagttaccg atcttgattc cgcattcgaa
      841 gttttgcggg acttccttga cgtcatcctt gaaacgtttg agggcttcga tcgagccttt
      901 gtagatttcg cgtccctcgc ggatcaagcg gaccttggcc gaacgttcga tggtgccctg
      961 ggtgacgaag cacccggcga cggcgccgac cttcgagatc ttgaaaacct ggcgcacgac
     1021 tgcccggccc aagtggacct cttttatctc ggggatgagc atcccttcga cggctttctt
     1081 gatgtcgtcg gtcacctggt agatgatgtc gtagcggcgg atctcgattt tcttttcgtc
     1141 ggcgagcgtg atcgcgcggt cttcaggggc aacgcggaag ccgatgacga tggcttgcga
     1201 ggcgtcggcc aggacaatgt cgctctcgga gattccgcca accgctttca agagaacgcg
     1261 gatcgggact tccgcgtttt caagcttttc gagttccttg gtgagtgctt cgatcgagcc
     1321 ctggacatcg gctttcaaga tcaggttcaa gctcttgagc ttttgctcgg ccatcttgtt
     1381 gtagaggttt tccagggtaa ccgactggcg atcggcatgg gcggcatcgc gggcacgggt
     1441 tcgccgggtc tcggcgatgt cgcgggcact ggtgatatcg tcgataaccc cgaagttttc
     1501 gccggcgctc gggacgatgt cgagtccgga gatctccacc ggtgtggacg ggccggccgt
     1561 ttcaatcgag cggcctttgt cgttgaaaat cgcgcggacg cggccaaaac catcgccgca
     1621 gacgatcaca tcaccgacct tcagagtgcc atcctgcacg agcaacgacg cgagtacgcc
     1681 gcggccctcg gacagagacg cctccaggca ggtgccggtc gcggggcgcg ttgggtcggc
     1741 cttgagctca tgcaattcgg cgatcgtctc gagcgtggcc aggaggtcgg tgatccccaa
     1801 gccagaagca gcggaggtct tgacgacctc ggtatcgccg ccccattcaa cggggttcag
     1861 cccctgagtt gatagctcgc cgtagatctt gttgatgttg gacggcgtat cgacgttggg
     1921 cagatcgatc ttgttcaagg ccaccacgat gggcacggcg gccgccttgg cgtgagcgat
     1981 ggcctcgacg gtctgtggca tcacaccgtc gtcggccgca acaaccagta cgacgatgtc
     2041 ggtgacgttg gcgcctcggg cacgcatcga ggtaaacgcc tcatggccgg gcgtgtcgac
     2101 gaacgtgatc ggacggccgt tagattcgac ctggtaagcg ccgatgtgct gggtgatacc
     2161 tccactctcg gagtcgacca cgtgcgactt gcgaattcgg tctaagagcg aggtcttgcc
     2221 atgatcgacg tgacctaaga tcgtgatcac cggaggtcga agctggaggt tttccgacga
     2281 gccagacgac tcgaacgaat cgagcagatc atcttcggcg gtgcgcatgt gaacaacctc
     2341 tagctcgatg ccgaactcga gcgcgagcat ggtcgccacg tcgtcgtcga gtgaagcatt
     2401 gatcgtggcg agctggttca ttgccatcag cttgcggatc aggtcgttgg ctttgactcc
     2461 gattgccagc gagaggctgc gaatgttgat cggtgcttcg atctcggcgt gcgacttgcg
     2521 cgatggggcg attgcgatcc gctggccgcc gcgcgacttt ttggtgccgg agcgggtacg
     2581 gcgtgtgtct tcatcatccg agaggagcgc tgcggcgggg actggcgagg tcacacggcg
     2641 atcaacggcg cgctcgctcc ggcgggcgcg acggcctgcg cgatcggcgg ccgagcccaa
     2701 gcggccggtc ttcttctttt cctcctcttc ctcggcggcc gtcatcgtgg gccctaaagg
     2761 cgctggcatt ccaggtccag gagccggacg ccgcggagcg gaacccggac ccagcattga
     2821 acctccacgg gggggtgagg acgaaggacc ggctccacgc ggcggctgcc cgctgcgcat
     2881 gcccgctccg gggcccgacg gacccccagg agattggaaa ttgccgagat tgccggactt
     2941 catccaggcc agcatttcct ccttggtcat cgacttttcc ggacgctggg tcttgggcgc
     3001 tggacccggg gcggtcgatc gtggcggacc gaccgtgcgt tgcggcgaag ttgtggccgc
     3061 ggccaccgtg ggcaacggcc ggcctgttgc tcgggatgcg tcacgcctcg tgctatcgcc
     3121 cccgagctcg ccaccggcac gacgcggccc ctgcgatggg ctcgtcgaag ggccaacccg
     3181 gggggccata ggtcgggtac ccgcggatga catgtagtcg tctctcttga gcggctggaa
     3241 gccagacgcg cccgctgtcg gcgacgacga ccccgtcgaa ggctggatgc ctctggggtc
     3301 gtgtcgcggt cctccaccgc ccggcggcgg gccaccgggt cgcgggcccg tgcctcgatg
     3361 cggcgtgtgg gccgagagcg gtccggcgga gggcctggtc ccggaaaaac caccgggacg
     3421 tgccagcgga gccgcgctca accctgacgg aggtgccgtt gccgggcgcg gcgcgcctga
     3481 gttaggcggt gacgccgcaa taccgaccgg cgaaggcggt ttcgcggatg ccgaggggga
     3541 cgagccgggg atcaatcgtt gcggaggcgc cgttgccggc accggcgtac tcgacgccgt
     3601 gggggctggc tccgccgcgc gtaccggctc gatcttggcc gaacccgcag ccaccggtgc
     3661 aggcccgacc ggacgggccg ggggtgctgg ccgttcttcg cccgcgctcg cacggggagc
     3721 aaccgtggcc gatgctgcca acgacgacgg cgcggccgca ggtttggcgg ccgaggccgc
     3781 ggttgccgac cccttaagag ccgccggctg gtccatcaac tcccgaatcc gatccaccat
     3841 gtccgggtcg agactcgcca gggcgtttgc cttgacgtcg agacc
//
LOCUS       DATAQL010000506         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_811849, whole genome shotgun sequence.
ACCESSION   DATAQL010000506 DATAQL010000000
VERSION     DATAQL010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811849"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..>2058
                     /locus_tag="VHS97_08725"
     CDS             385..>2058
                     /locus_tag="VHS97_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448323.1"
                     /translation="MARLFVRFLCTFAFVAATTHCGAADRVVFTRLGPTQSVLFIANA
                     DGSAERPLTQRDSLNYNPAWSPHDDWIAFTSERAGPANLFRMHPDGSDVQRLTDDPAY
                     DDQAAFSPDGGQIVFVSTRSAGFANLWILDVARRKARPLTTGRGGDFRPAWSPDGEWI
                     AFSSDRDSDLPTAKSRWERLHLVDIYLIHPDGSGLKRISRHGNFCGSPRWTADSKSVV
                     TYCMPAQDTWTYRTKLLTPYEAGGENQLYRIDIGTGATVPVTSGPGVKLAPSVLASGQ
                     IAYSRGDGANIELISADGKVGPSGTDIAPEPVAWSPDGRRVVYTRQAFQPSSQPAKQW
                     SRDPKFDLYATTELPAYDRTGTRLAVSNFVPGGVTSLEIIDGDQPGRAILKRNDLILG
                     PQWSPDSRQLVVGVGNFTSFLDFAAGTKTPIDRLNGGAQVAILNADGTGFHVITSGPS
                     NNAFASFSPDGRKIVYRTAGPAGQGLRTMNLADHSVTPLTSEYDNFPVWSPRGDIIAF
                     VRNIGGDFEVMTIHPDGKHVSQLTHTHGNEAHLAWSPDGERLLFTSSRMG"
BASE COUNT          384 a          663 c          606 g          405 t
ORIGIN      
        1 cagtgaatcg taaacgggta acttattgca attaggcgtc acggatggct accagagcgc
       61 tctaaggaac cctgagaacc cgtcattgca cccttaacaa acccacgacc tctgccgggc
      121 tgacctctgc ccaacccgat caaccgtggc ttctgggtgc gagcgcgatt atcgtcatca
      181 gttcccccct cgagggcacg ggcactatac aggcgttcga cgagtgactc gccctgtaat
      241 cagcaattgg cacctaggga ttgctgagtt tgggccgtgg cgacagcctc ctgatctacc
      301 gaatgtaaat ttccagccat ctttgcctct ggtagcatcg gaccggtttc ctgcctatca
      361 gaatccatct ctcggagcga tacaatggct cgccttttcg tccgattcct ttgcacgttt
      421 gcatttgtgg cggcaacaac gcactgcggc gcggccgacc gggtcgtctt cactcgctta
      481 ggaccaacgc aatccgtcct cttcatcgcc aacgccgacg gatcggcgga gcggccgctc
      541 acgcagcgcg actcgctcaa ctacaacccc gcctggtctc cccacgacga ttggatcgcc
      601 ttcacctcgg agagggcggg acccgccaat ctcttccgca tgcatcctga cggcagcgac
      661 gtacagcgcc tgactgacga tccggcgtat gacgatcagg ccgcgttctc tcccgatggc
      721 gggcagatcg tcttcgtatc cacgcggtcg gcggggtttg cgaacctgtg gattctggat
      781 gtcgcccgcc gcaaagcgcg ccccctgacc accgggcgag gtggcgattt caggccggcg
      841 tggtcccccg acggcgagtg gatcgccttc tcttccgacc gcgacagcga tcttcccacc
      901 gcgaagagcc gttgggaacg gcttcatctg gtggacatct atctcattca tcccgacggc
      961 tcggggctta agcgcatttc gcggcatggg aacttctgcg gcagtccgcg gtggacggcc
     1021 gacagcaaga gcgtggtgac ctattgcatg ccggcgcaag atacctggac gtatcggacc
     1081 aagctgctta cgccgtatga ggcgggcgga gaaaaccagc tgtacaggat cgacatcggc
     1141 acgggcgcga ccgttcccgt aaccagcggc cccggcgtca aactggcgcc ttccgtgcta
     1201 gcctccggcc agatcgccta ttcgcgcggc gacggagcca acatcgagct tatctccgca
     1261 gacggcaaag tgggcccctc cggtaccgac atcgcgccgg agccggtggc ctggtctccc
     1321 gatggccggc gtgtcgtcta cacccgccag gctttccagc ccagttctca gccggcgaag
     1381 caatggagcc gagatccgaa gttcgatctc tacgcgacga ccgagttgcc ggcctacgat
     1441 cggacgggga cgcgtctggc ggtctccaac ttcgtccccg gaggcgtcac cagcctggag
     1501 atcatcgacg gcgaccagcc ggggcgcgca attctgaagc ggaacgatct gattctgggt
     1561 ccgcaatggt cgccggatag ccgtcaactc gttgtcggtg tcggcaactt tacgtccttc
     1621 cttgactttg cggcgggcac caagacgccc atcgatcgcc tcaacggagg agcgcaggtc
     1681 gcgattctca atgcggacgg caccggcttt catgtgatta cctcgggacc gagcaacaac
     1741 gcctttgcct ctttctcgcc ggacggaaga aaaatcgtct atcggaccgc cggacccgct
     1801 gggcaaggct tgcgcaccat gaatcttgcc gaccactctg tgacgccgtt gaccagcgag
     1861 tacgacaact ttcccgtgtg gtcgccgcgc ggcgatatca tcgcctttgt gcggaacatt
     1921 ggcggcgact ttgaagtgat gacgattcat ccggatggga agcacgtgag ccagttgacg
     1981 cacacccacg ggaatgaggc gcacctggcc tggtctcccg atggggaacg gctgttgttt
     2041 acgagttcgc ggatggga
//
LOCUS       DATAQL010000507         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3714587, whole genome shotgun sequence.
ACCESSION   DATAQL010000507 DATAQL010000000
VERSION     DATAQL010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3714587"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..600
                     /locus_tag="VHS97_08730"
     CDS             49..600
                     /locus_tag="VHS97_08730"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX3448324.1"
                     /translation="MFASSAVPVIVVLTAALIAAVTDIWKFKVYNALTLPLLAAGLLY
                     HGIRFEIADSLIGILFGFAALVPLYIIGGMGAGDVKLMAAVGAWLGWPLTFYVFIASS
                     LAAGAYAVMLMVATHKVGETIVNLHILWLRLGSIGRYLGSDDRVEAEVRRTDRRRRII
                     PFAAMVAIGIVATIFWFRSELLR"
     gene            704..1672
                     /gene="cpaB"
                     /locus_tag="VHS97_08735"
     CDS             704..1672
                     /gene="cpaB"
                     /locus_tag="VHS97_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX3448325.1"
                     /translation="MSPRSLLIGVLALVFGGSAAVGVNSLIRNAKAPSGDVVTVVVAA
                     ADVPRGGTLTAELVKTRDYPTDLVPVGALSKVEDAVDRAVFIPLTKGDPVLNTKLAAK
                     GSGRGMSALVPKGMRAFTIHTPSIASGVAGFVLPGNKVDVLLTVVDLPAITVANSARA
                     TGGSSTTTLLQNVEILAVDQKIEAPAENKMDSHQLRSVTLLVTPQHANLLELGQSKGT
                     LHLTLRNIEDKDDARTRPATLTDLQFHQEPPWDERAKSVLEALGRALAQRQPVPPPAP
                     APPKAPEPPPTALIRTVRGVREGGVIVHVPVAGPVVTDLRNRSPRG"
     gene            1676..>2686
                     /locus_tag="VHS97_08740"
     CDS             1676..>2686
                     /locus_tag="VHS97_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448326.1"
                     /translation="MQALVVGDHEVMTAQIRQVLLREGQDCPVSHVVRLDSAAGRLEQ
                     VKIELAVVALSPDPERALAVVTAIRTKVTGRMLVVGPAADSKLVLRALRGGADDYIDE
                     LELVAELEDAVLRLKSQMTAHSSDTGRTIAVLAPSGGSGSSTLAANVATVLAKEHKSA
                     LLCDLKLESGDLAALLDLKPTHSLADPCLNVQRMDRVLFERSLVRHSSGVHLLAAPLH
                     YSEVAHITAESVRKVLNLGRTLFPYVVIDLDHNFHAEQVDTLHESDLILVVLRLDFAS
                     LRNARRTLEQLERLGVDREKLRLVVNRYGQPKEVPFAKAEEALGQNIFHYVPDDPHAV
                     NRA"
BASE COUNT          458 a          860 c          846 g          522 t
ORIGIN      
        1 cccatttcct ttgatcgctg cggcatgtct gatctcggag agtgagctat gtttgccagt
       61 agtgctgtgc ccgtgattgt cgtcttgacc gccgctctca tcgcggcggt taccgatatc
      121 tggaagttca aggtttacaa cgccttgact ttgccgctgt tggcggccgg cctgctttat
      181 cacggaattc gattcgagat cgcggacagc ctgatcggga ttcttttcgg gttcgcggcc
      241 ctggttccac tgtacatcat cggcggcatg ggggccggcg acgtcaagct gatggcggcc
      301 gtcggagcct ggctgggatg gccgctcacc ttctacgtgt ttatcgccag ctcgctggcc
      361 gcgggggcct acgcggtgat gcttatggtg gcgacccaca aggtcggcga gaccatcgtc
      421 aacctgcaca ttctttggct gcgattgggg tcgatcggcc gttatctggg ctccgacgat
      481 cgggtagagg ccgaagttcg gcggaccgac cgccgccgca gaatcatacc gtttgccgcc
      541 atggtggcga tcggcatcgt cgcgacaatc ttctggttcc ggtccgagct cttgcgctga
      601 cccggaaact ccggcgggct ccggcggggc cgctggattc gtcacatgtc agatttcgac
      661 gagtattctt ctttaaatgt aatccttcgg gaagaggtga tccatgagtc ctcgatcctt
      721 actgatcggg gtgctggcgc ttgtgttcgg cggctcggcc gccgtcggcg tcaattcctt
      781 gatccggaac gcgaaggctc cgtctggcga cgtggtgacg gtggtcgtgg ccgccgcgga
      841 tgtgccgcgc gggggaacgc tcacggctga gctggtgaag acgcgcgatt atcccacaga
      901 cttagtcccc gtgggcgcgc tttcaaaggt tgaagacgcg gtcgatcggg ccgtcttcat
      961 ccccctgacc aagggcgacc cggtcttgaa taccaaactg gcagccaagg gctctggccg
     1021 cggcatgtcc gcgcttgtcc ccaaggggat gcgagccttc acaatccaca cgcccagcat
     1081 tgcctcaggc gtggctgggt ttgtcttgcc cggaaacaag gttgatgtgc tcctcaccgt
     1141 cgttgacctg cctgcaatca ctgtagccaa ttccgccagg gcaaccgggg gcagcagcac
     1201 gaccacgctg cttcagaacg tcgagatcct ggccgtcgac cagaaaatcg aggccccggc
     1261 cgaaaacaag atggactcac accagcttcg ctctgtgacc ctcttggtca caccgcagca
     1321 tgcaaacctg ctcgagctgg gtcaaagcaa gggaacattg cacctcacac tccggaatat
     1381 tgaagacaag gacgacgcgc gcacccgtcc cgcgaccctg accgacctcc agttccacca
     1441 ggagccgccg tgggacgagc gggccaagtc cgtactcgag gcactggggc gggccctggc
     1501 gcagcgccag cccgtgccgc cgccggcacc agcgccccct aaagcccccg agcctcctcc
     1561 cacggctttg atccggacgg tgcgcggcgt ccgggaaggc ggcgtgatcg tccatgtgcc
     1621 ggtagccggc ccggttgtca ccgatttgcg aaatcgatca cctagaggat aatcgatgca
     1681 ggctctggtc gtgggtgatc acgaagtgat gacggctcag atcaggcagg tcctgctccg
     1741 cgaggggcag gactgcccgg tctcgcacgt cgtccggctc gacagcgctg ctggccggct
     1801 tgagcaggtc aagatcgagt tggccgtggt ggccctgtct ccggaccccg agcgagcgct
     1861 cgcagtggtg acggcgatcc gcaccaaggt caccggccgg atgctggtcg tcgggccggc
     1921 cgccgactcc aagctcgtcc tgcgagccct ccggggcggg gccgacgatt acattgatga
     1981 gctcgagctc gtcgccgagc tcgaggacgc cgtgttgcgg ctgaagtcgc aaatgacggc
     2041 ccactcgagc gataccgggc ggaccatcgc cgtgctggca cccagcggcg gcagtgggtc
     2101 gagcacgctg gcggctaatg tggcgaccgt gctggccaag gaacacaagt cggccctgct
     2161 ttgcgacctc aaactcgaaa gtggcgacct ggcggccttg ctcgacttga agccgaccca
     2221 ttccctggcc gatccttgcc tgaacgtcca acggatggac cgcgtgctct tcgagcggtc
     2281 gctggtgcgg cactcgagcg gggtgcacct tctggccgcg cccttgcatt atagcgaggt
     2341 cgctcacatc acggccgagt cggtccgcaa ggtcttgaac ctgggccgca ccctctttcc
     2401 ctacgtcgtc atcgatctcg atcacaactt ccacgccgag caggtcgaca cgctgcacga
     2461 atcggacctt atcctcgtgg tgctccggct cgacttcgcc tcgttgcgca acgcgcggcg
     2521 caccctcgag caactcgagc gcctgggtgt cgatcgggaa aaactgaggc tggtggtcaa
     2581 tcgctatggc cagcctaaag aagtgccgtt cgccaaggcc gaagaggccc tggggcagaa
     2641 catcttccac tatgtgcccg acgaccctca tgcggtcaac cgggcc
//
LOCUS       DATAQL010000508         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3868003, whole genome shotgun sequence.
ACCESSION   DATAQL010000508 DATAQL010000000
VERSION     DATAQL010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3868003"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="VHS97_08745"
     CDS             complement(<1..99)
                     /locus_tag="VHS97_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX3448327.1"
                     /translation="MEWLESLVLGLVQGITEFLPVSSDGHLLLTQNL"
     gene            complement(152..754)
                     /gene="pgsA"
                     /locus_tag="VHS97_08750"
     CDS             complement(152..754)
                     /gene="pgsA"
                     /locus_tag="VHS97_08750"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX3448328.1"
                     /translation="MSRAENRFAMVAPATRRFWNVPNTLTVSRLVLAVCVFAFIDYQL
                     YFWALALFVVAAITDALDGYFARLLKQDTPIGRQLDPLIDKVIVAGCYIYLAAIPGTG
                     VLPWMVTAIVVREILIQGLRSHLEGQGQPFGARTAGKLKTVVQCLSISAVLFCLGLVP
                     APPVSVLWIRDILTWLAVALTLYSGFLYIWVAFPKLRGEA"
     gene            complement(834..2189)
                     /gene="rimO"
                     /locus_tag="VHS97_08755"
     CDS             complement(834..2189)
                     /gene="rimO"
                     /locus_tag="VHS97_08755"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX3448329.1"
                     /translation="MLGLLGQDGYALVPDSQQSDLVIINTCGFIDAARRESIGVIEEM
                     LERKRSGHVRGVIVAGCLAERQKDDLLEQFPDVDHVVGVFGRDEIARVADRLMGGLDE
                     QRTLFRPAPIQAQDDRARLRITPRHLSYLKVSEGCDRFCTFCAIPLMRGKHITKPIEM
                     VVAEAEELAADGVRELILVAQDMTYYGLDLYGRTRLAELLREVGKVEGIDWIRILYCY
                     PQFFTDELYDVLSNTPKIIPYLDMPLQHINDRMLKMMNRRHNRQETEAIIARLRATIP
                     KLVLRTTLIVGFPGETEAEFSELLDFVAATRFERLGAFTYSFEPDTPAAKLPDQLPDQ
                     VKTDRQARLMGVQQPIAFAFNQSLVGRTLDVLIDTAAPEEKHLWLGRTYTDAPDVDGN
                     TWVRGAQFQPGDLIECEIVAAQEHDLVARPVSNTPPKKRRARPRPRRRPQSALPILDG
                     M"
     gene            complement(2123..>2802)
                     /locus_tag="VHS97_08760"
     CDS             complement(2123..>2802)
                     /locus_tag="VHS97_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448330.1"
                     /translation="QATAGGCQWMLDRWSELRSILEEGLDWQSADKLKAVRLLGRHPI
                     EAVDDRNALMVFVACQSVESRTSIQIPDIWNELRKYERKQYAERLIGRGVEKLRPTDT
                     AAARQVLYAIIDRATAQIALKAEAHRARAEIDERTRLLAWNASIDDNEYFRPRVGLNR
                     LRLCFTRWRHLLFPDVGRKVPCRRHLRFPSLAWAARKTWSIASGCLGSLARTGTRSYP
                     ILSSPTW"
BASE COUNT          570 a          843 c          856 g          533 t
ORIGIN      
        1 caaattctga gtgagcagca agtggccgtc gcttgagacc ggcaagaatt ccgtgatccc
       61 ctggaccaaa cccagcacca gcgattccaa ccattccatt attgttagct aacctcaagt
      121 gatgatgtgc gggttacgtg aataccacaa ttcatgcctc acctcgcagc ttggggaacg
      181 caacccagat atacaaaaat ccgctataca gcgtcagcgc gacggccagc caggtgagta
      241 tgtcgcggat ccacaagacg cttacagggg gagcaggtac gagcccgaga cagaaaagga
      301 cggccgagat cgacaggcat tgtacgacgg tcttcaactt gccggcggtc cgtgccccga
      361 acggctgacc ctgaccttcc agatggctgc gcagaccttg aatcaggatc tcgcggacga
      421 cgattgccgt gaccatccag ggcaggaccc cggtgccggg aatcgctgcc agatagatat
      481 aacagcccgc cacaatcacc ttatcgatca aggggtcgag ctggcggccg atgggggtgt
      541 cttgtttcaa gagccgggcg aaataaccgt cgagggcgtc ggtgatcgct gcgaccacaa
      601 acagagcaag cgcccagaaa tagagctggt agtcgatgaa ggcgaacacg cagaccgcca
      661 gcacgagccg actgacggtg agcgtgttcg ggacgttcca gaatctccgg gtcgccggtg
      721 cgaccatcgc gaagcggttc tcggctcggc tcatgagaga catccgactc gcggggacga
      781 cagagaccct tcttatgggt gcaaaggcca gaatggacga gcaacgcgaa cgcttacatc
      841 ccatcgagga tcggcagcgc cgactgaggc ctgcgccgcg gtcgcggtcg agccctccgt
      901 ttcttcggtg gagtgttcga taccggccgg gcaaccaggt cgtgctcttg cgcggccacg
      961 atctcacact cgatcagatc tccaggctga aactgagccc ctctgaccca tgtgttgccg
     1021 tcaacatcgg gtgcgtcggt gtaggtacgg ccgagccaga gatgcttttc ctcgggagcg
     1081 gccgtgtcga tcaagacatc cagggtccgg cccaccaagc tctggttgaa tgcaaaagcg
     1141 atcggttgct gcacgcccat cagtcgcgcc tggcgatcgg tcttgacttg atcgggcagt
     1201 tggtccggca gtttggccgc aggcgtgtcg ggctcgaacg aataggtaaa cgcgccgagt
     1261 cgctcaaacc tggtcgccgc gacgaagtcg agcagttcgg aaaactcagc ctcggtctcg
     1321 cccggaaagc cgacgatcaa agtcgtgcgc aaaaccagct tagggatggt ggcccgcagt
     1381 ctggcgatga tcgcctcggt ttcctggcga ttgtgccgcc ggttcatcat cttgagcatg
     1441 cgatcgttga tgtgctgcag cggcatatcc aggtagggaa tgattttcgg cgtgttgctg
     1501 agcacgtcgt agagttcgtc ggtaaaaaac tgcggatagc aatagagaat gcggatccag
     1561 tcaatccctt cgaccttgcc cacctcccgc agcagctctg ccagtcgagt ccggccatag
     1621 aggtccagtc cgtagtaggt catgtcttga gcgaccaaga taagttcgcg gacgccgtcg
     1681 gcggccagct cttcggcttc cgcgacaacc atctcgatcg gcttggtgat gtgcttgcca
     1741 cgcatgagcg ggatcgcgca aaacgtgcag aatcggtcgc acccttcgga gacctttagg
     1801 taggagaggt ggcgcggggt gattctcagc cgggcgcgat catcttgcgc ctggatgggt
     1861 gccggtcgaa acagcgttcg ttgctcgtcg agcccaccca tcagacggtc ggccacccgg
     1921 gcgatctcat cgcggccgaa cacgccaacg acgtggtcga catccggaaa ctgttcgagt
     1981 agatcgtctt tctgacgctc ggccaggcag ccggccacga taaccccgcg gacgtgcccc
     2041 gaccgctttc gctcgagcat ctcctcgatc accccgatcg attcccgacg cgcggcgtcg
     2101 atgaacccgc aggtattgat gatcaccagg tcggactgct gagaatcggg tacgagcgcg
     2161 tacccgtcct ggccaaggag cccaagcatc cgctcgctat cgaccaggtt tttcgagcag
     2221 cccaggctaa cgaaggaaat cgtaggtgtc ttcggcatgg aaccttccgg ccaacgtccg
     2281 ggaaaagcag gtgtcgccat cgtgtaaaac agagcctcaa gcgattgagg ccgacgcgcg
     2341 gtcggaagta ttcattgtcg tcaatcgacg cgttccacgc cagtagtcgg gtgcgttcgt
     2401 cgatctccgc ccgggcgcga tgcgcctcgg ccttgagtgc gatctgggcc gtcgcgcggt
     2461 cgatgatggc gtaaaggacc tggcgcgcgg ccgcggtatc ggtgggcctg agcttttcga
     2521 cgccccgccc gatcaggcgc tccgcgtact gcttcctttc gtatttgcgg agctcgttcc
     2581 agatatcggg aatctggatg ctggttcgag actcgaccga ctgacaggcg acgaacacca
     2641 tgagcgcatt ccgatcgtcg accgcctcga tgggatggcg gccgagcagt ctgacggcct
     2701 tgagcttgtc ggccgactgc caatccagtc cttcctcgag aatcgacctc agctcggacc
     2761 agcggtcgag catccactgg cagccgccgg ccgtcgcttg ca
//
LOCUS       DATAQL010000509         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2492220, whole genome shotgun sequence.
ACCESSION   DATAQL010000509 DATAQL010000000
VERSION     DATAQL010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2492220"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VHS97_08765"
     CDS             complement(<1..346)
                     /locus_tag="VHS97_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3448331.1"
                     /translation="MPRVVVFGSSNTDMTLRLPELPVPGQTVLGSTFSTTPGGKGANQ
                     AVAARRAGANVVFVTAVGHDDLGKRSLDVYESEGIDVSHIRILEGVASGVALIFVDET
                     GENMIGVASGANL"
     gene            complement(441..815)
                     /locus_tag="VHS97_08770"
     CDS             complement(441..815)
                     /locus_tag="VHS97_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3448332.1"
                     /translation="MIVPVPDENQKQPQAGDSYFIPAGSGSRHQIFPGVEIQTTAGAN
                     LMLSVVRFEPESVVLEHAHPHEQIGILLEGRLEFTVGGVTRLLGPGDKWRIPGGVVHS
                     VRALDQAAVALDVFHPIREDYL"
     gene            complement(812..2392)
                     /locus_tag="VHS97_08775"
     CDS             complement(812..2392)
                     /locus_tag="VHS97_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX3448333.1"
                     /translation="MSTIQRSSGLTRLLLFTAVGLVPIGLSCGYPRCLAQGYGGGQDP
                     GELEMPYVGPLPGDFDATRRRATRTKTAKKKSALAEIGASKKADASGKAKTADTKKKS
                     AGSSQIKFSQEIAPILVANCTGCHSGDKAGARRGKLDLTTFEKLTNGTPDHKIVVPGK
                     PAESHLVMRIKGEEKPRMPQGNNGVLSPEAIAKVEQWVKEGAKLDDGIDPKKMVASYA
                     ASVDQMRRSRLAKQPAQDRDKNVEAVGLERWKLSNAKIKPDIVRGEHFIMFSNLPSDR
                     ASSTLKVLDKQPGHLKRYTGLPTTDRVEKISLFVFSNRKEFVEFVRSVEKNEVEADTL
                     SSGQLSVDQPYLAVVDPAGGKKEESASSKRKGGRSRRNDEKDMDSTGSDRTLAGFLTE
                     TLGKATVESAGKPPRWLVVGIGSYFASLVEPQSHRYRQLRQTAFANFSQGWETRANNV
                     LGQTDQVTVDGINAIGFALVEAMMSEMRQGFPAFVMGMAQEGNNLDQTLQQVYGGNRG
                     EFLSGTGEWVAAHYGNLE"
     gene            complement(2549..3802)
                     /locus_tag="VHS97_08780"
     CDS             complement(2549..3802)
                     /locus_tag="VHS97_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448334.1"
                     /translation="MHPVSISGRSMTRARQMFRALLVMGGILSTSVSAGCLRSTMLRP
                     GDGPVPQSPPSSEPTSDRRSDAPAGPSPRQGGELSASPVGAPVARSAETAPAAATSTP
                     PSEPQAGTPQKIQSATDSSGRQPVAVAPEITVSETPVPNPTPLLDAAIERVAAVTRQD
                     ESVDPGSATFPSKNGVPRADAQGPPTGATIAPLQLSEGPRTADTPVSNPAIGPSLIKQ
                     NDPPAAIEVFVVPKAETPSVRVENEIKSDTVAKTGSSSPSMDSSPKSDERVHAAIDIT
                     KPEPVNAEPDPLGINKLMLCRKVLGFGSFESLADTRVKAGQRLLVYCEMTGMQYEEKH
                     LEFVSRLSSRIEITSIANGTIVWMHELGPAEDVCSSRRRDFYVNFRVDLPSSLPIGLY
                     SLRLTQSDLVAQRSTSAEIPFEIVP"
BASE COUNT          778 a         1223 c         1239 g          887 t
ORIGIN      
        1 tgaggttggc gcctgacgcg acgccgatca tgttttcgcc ggtctcgtcg acgaagatga
       61 gcgccacgcc cgacgcgact ccttccaaga tccggatgtg gctgacgtcg atcccttcac
      121 tttcgtaaac atcgagcgac cgcttgccca ggtcatcgtg gccaacagcg gtcacgaaca
      181 caacgttggc tccagcgcgc cgcgccgcga cggcctggtt ggcccctttg ccaccgggag
      241 tggtggaaaa cgtgctgccc aaaacggtct ggccggggac tggcagctca ggcagccgca
      301 gcgtcatatc ggtgttgctc gaaccgaaga cgacgacacg gggcatcgac gagcttcccc
      361 tgggtgtttc tcaattccag aatcgccgca tccagtttac cggcagctgg attgacgatt
      421 tccagttcac cggcctggta ttacaggtaa tcttcgcgga tcggatggaa aacgtcgagt
      481 gccacggccg cttggtcgag tgcccgcacg ctgtggacga ctccgccggg gatgcgccac
      541 ttgtcgccgg gaccgaggag acgggtaact ccgcccacgg tgaattcgag ccgtccttca
      601 agcaagatgc cgatttgctc atggggatgg gcgtgttcga gcaccaccga ttccggctcg
      661 aagcgcacga ccgaaagcat cagattcgcg ccggcagtcg tctggatctc gacaccggga
      721 aagatctgat gtcgcgaccc ggaccccgcc gggatgaaat aggaatcgcc ggcttgcggt
      781 tgcttttggt tttcgtctgg gaccgggacg atcattcgag atttccgtaa tgcgccgcga
      841 cccactcgcc agttccgctg aggaactctc cgcggttgcc tccgtacacc tgctgaagcg
      901 tttggtcgag gttgttccct tcctgagcca tccccattac gaatgccgga aagccctggc
      961 gcatctcgga catcatggcc tccaccagtg caaacccgat cgcgttgatc ccatccacgg
     1021 tgacctggtc ggtttggcca agtacgttgt tggctctcgt ctcccagccc tgactgaaat
     1081 tcgcgaaggc ggtttgacgc aattgccggt aacggtggct ctgtggctcg accaacgacg
     1141 cgaaataaga gccgatgccc accaccagcc agcgcggcgg tttgccggcg gattcgaccg
     1201 tggccttgcc caaagtctcc gtgagaaatc ccgccagagt tcggtcgctg ccggtgctat
     1261 ccatatcctt ttcgtcgttt cgtctggacc gaccgccctt ccgcttggag gaggccgatt
     1321 cctccttttt gccccccgcc ggatcgacga cagccagata aggctggtcg accgacaatt
     1381 ggcccgaaga cagtgtgtcg gcttcgactt cattcttctc aaccgaccgg acgaattcga
     1441 cgaattcttt tctattggag aagacaaata ggctgatctt ttcaacccta tcggtcgtcg
     1501 gcaaaccagt gtaccgcttc aagtgacctg gctgtttatc cagaaccttg agcgtactcg
     1561 aggcccgatc gctgggcaaa ttgctgaaca taatgaagtg ttcaccgcga acaatgtcgg
     1621 gcttgatctt ggcgttggac agcttccagc gttcaaggcc tacggcttcg acattcttat
     1681 cgcggtcctg cgcaggctgt ttggcgagcc tgctccggcg catctgatcc acgctcgcag
     1741 cgtacgacgc gaccatcttt ttggggtcga tcccgtcatc gagtttagcc ccctctttga
     1801 cccattgctc gaccttggcg atcgcttcag gcgacaggac cccgttgttg ccttggggca
     1861 tgcgcggttt ttcttctccc ttgatccgca taaccaagtg actctcggca ggtttgccgg
     1921 ggacgacgat cttgtggtcg ggcgtaccgt ttgtcagctt ctcgaatgtc gtcaaatcga
     1981 gttttccgcg tcgtgccccg gctttatctc cactgtggca cccagtgcaa ttcgcgacca
     2041 aaatcggtgc aatttcttgt gaaaacttga tttgactcga gccagccgac ttcttttttg
     2101 tgtccgctgt tttggccttt cctgatgcgt cggccttttt ggaggcgccg atttcggcaa
     2161 gggcgctctt cttcttggca gttttcgtcc gcgtggcgcg gcgacgcgtc gcatcgaaat
     2221 cccccggcaa tgggccaacg tagggcatct ccagctctcc ggggtcttgt ccgccgccgt
     2281 agccttgggc caggcaacgg ggatagccgc aggacaaccc gatcgggaca agcccgaccg
     2341 cagtgaagag caagagcctg gtaaggccag atgatcgttg gatcgtggac atgatcttgt
     2401 ccccgttggc atgtcggaac cgcgcgattg gatcacatca ttgtctaccg cttgggcgga
     2461 gttggcaaga tgcgtaaatg gctgtgttcg cgacgcaacc cgacaagcta aggcagtcgg
     2521 ccaatagcgg cccggcacgt tgtgtttttc aggggacgat ttcgaacggg atttcggccg
     2581 aagttgagcg ctgcgcgacc agatcactct gcgtcagacg gaggctgtac aggccgatag
     2641 gtagtgatga tgggaggtcg acccgaaaat tcacgtagaa atcacgacgg cgactactgc
     2701 acacatcctc ggcgggcccc agttcgtgca tccacacgat cgtgccgttc gcgatcgagg
     2761 tgatctcgat tctcgatgag aggcgcgaaa cgaattccaa gtgcttttcc tcgtactgca
     2821 tcccggtcat ttcgcagtag acaagcagcc gctggccggc tttgacacgc gtgtcggcca
     2881 aggactcgaa cgatccaaag ccaagtacct tgcggcacaa catcaatttg ttgattccaa
     2941 gtgggtcggg ttcggcattt acaggctctg gcttggtgat gtcgatcgcc gcgtgcactc
     3001 gttcgtccga tttcggtgac gaatccatcg acggtgaaga ggatccggtt ttcgcgacgg
     3061 tgtcggactt tatctcgttt tccactcgaa cggatggcgt ttccgccttc ggaaccacga
     3121 agacttctat cgcagcaggc gggtcatttt gtttgatgag tgacggacca atcgcggggt
     3181 tcgacaccgg ggtgtcggcc gtccgcggac cttcagatag ttgtaacggc gcgatcgtag
     3241 caccggtcgg cggcccctgt gcgtcggcac gcgggactcc attcttggag gggaatgtgg
     3301 ccgacccggg gtcgactgat tcatcttggc gagtgacagc cgccacgcgc tcgatcgcgg
     3361 cgtcgagaag gggtgtagga ttgggcacgg gggtttcgct gaccgtgatc tcgggagcga
     3421 ccgcgaccgg ctggcgaccc gacgagtccg ttgcggattg aatcttttgc ggcgtgccag
     3481 cctgcggctc actcggcggc gtagacgttg cagccgccgg tgctgtttcg gccgaacgtg
     3541 ccacgggggc accgactgga ctcgcggaga gctcgccacc ctgccgcggg ctgggtccag
     3601 cgggagcgtc cgaccgacga tcggaagtcg gctcggacga tggcggggat tgggggacgg
     3661 ggccgtcgcc tggccgcagc atcgtcgacc gcaaacaacc agccgaaacg gatgtgctga
     3721 gaatcccccc catgacgagg agagcccgga acatctggcg agcgcgcgtc atcgaacgac
     3781 cgcttatgga aaccgggtgc atcgctccca cgctccggat tacccgcgga cacgcgggat
     3841 gaagggaccg tgacctagga cggaaatgcg gttgtcattt aactcaagtt ccagaatccg
     3901 gcaagctcaa actcgcatag agcgagcttc gggtgcgccg gtggaatctc gtatagcctc
     3961 cggcagaatc gaaacgcgca cggcggcgga attgaggttc gcaagcccga cccgcaagcc
     4021 gaagtttctc gaattacact ccagacagcg agcgctttga ccgtaagtcc gcatgcgagc
     4081 ctgcaaagtt acgtcaaaaa aaacgaatgg cactcctgtg tcgctcg
//
LOCUS       DATAQL010000510         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3563432, whole genome shotgun sequence.
ACCESSION   DATAQL010000510 DATAQL010000000
VERSION     DATAQL010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3563432"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..2129
                     /locus_tag="VHS97_08785"
     CDS             537..2129
                     /locus_tag="VHS97_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX3448335.1"
                     /translation="MRILLYNPDNGVTQNFMPHLWMFLLQALTPSGHEVVLIDGNTQP
                     MSDAELVQFVLDQQIGLVGIGAMTRMIAKAYRVADAVRVAGIPVVMGGPHVTEIPDEA
                     LGRSGGPRHADAVALGEADETWPRIVEDGARGELKEVYAPVDLFGEEHKPTLQDYPAI
                     PWHSVDLRQFNRIPGFFRPIMRRFGFEWETFHIIPIESGRGCPYGCEFCTVTGFFGDS
                     IRFRSNQSIVDEMLRIKDRARGTRGKAAVFFVDDNFAINVKRTKALLRDIIAAGAKLA
                     WVGQISANLLRDEELLDLIAASGGKWIFIGMESLDPANLASVNKSFNKPADYDRVLQA
                     LALRHVYAITSFIFGLDNDTPGVAERTLSEIRAWPPGLPVFGQITPFPATPLYERLKR
                     EGRLTRPNHWLEFAPFQMAHTPLKITISAVQNEVRYAWVNSYSPAAIERAIESIVDEP
                     AAYKISHLMSRLFFRGIYFPQKGAWDWFKLAVQNRRAISRVIRDSLTRWHGDQDPTRR
                     LDFNGSPESPFRDAAEQSAGAD"
     gene            2480..2944
                     /locus_tag="VHS97_08790"
     CDS             2480..2944
                     /locus_tag="VHS97_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3448336.1"
                     /translation="MTVLRNPDHVTIAVANAAAAIEFFEVLGFRKQHVAMIDGGPPAR
                     YMGMPDMKAQHITLALEGAEPRFEIQLLEFDPRPPDDLGEHPTNLRRRGYNHLAFRVD
                     DIGAATSHLVATGVKMLSDEMDYISRRVRFFEGPEAITLELVQWVDSDSGKG"
BASE COUNT          706 a         1009 c         1054 g          648 t
ORIGIN      
        1 ggccttcttg tccacagcta atcgtcgacc gagcgttccc agtgcgataa tctgagcatg
       61 aacaaaacga gcgccataat tggtgcggcc atggcgatct tcgttatcat cgtcactcgt
      121 atgcggggca ggttccgcta agagggctca ctctccaata accgcgttta ccatgcccga
      181 gcacttgtcc gacaaggcga caaaccggca acttggatgt cgtgagcgac gccgcacttg
      241 aggaatctcg cggcggaact tcccggaacg ggtctggccc gctcctgcga gcagacatcg
      301 acgggtctcg cgttcggtgt gccagggtga agttcagaag tcggcgcttg accgatgcgt
      361 cctatgaaca aatgatggcg cttcctgtgg ctggcgccta cctagctgat agaatggctt
      421 caaccgacac caactcctgc tcgatagcac gaacctcaac atgccgaagc tcgggctgct
      481 tgccggggaa ggatatacat gagcgttccg ctgcgacgct gcaaccggca aggaacatga
      541 gaatcctgct ctacaatccc gacaacggcg tcacccagaa cttcatgcct cacctctgga
      601 tgttcctgct ccaggcactg acacccagcg gccacgaggt ggtgctgatc gacggcaaca
      661 cgcaaccgat gagcgacgcc gaactggttc agttcgtcct cgaccagcag atcgggctgg
      721 tcggaatcgg cgccatgacc cgtatgatcg ccaaagcgta ccgcgtggct gatgccgtcc
      781 gcgtagccgg cattcccgtg gtgatgggtg ggccgcacgt gacggagatt ccggacgaag
      841 ctctcgggcg cagcggagga ccgcgtcacg ccgacgcggt ggctctgggt gaagcggacg
      901 aaacgtggcc gcgcatcgtg gaggatggcg cacgcggcga actcaaagag gtctatgctc
      961 cggtggacct gtttggggag gagcacaagc cgaccctgca ggactacccg gcgattccct
     1021 ggcactcggt cgatttgcgg cagttcaatc ggattccagg ctttttccgc ccgatcatgc
     1081 gccgcttcgg cttcgagtgg gagacctttc atatcattcc catcgaatcc ggccgcggct
     1141 gtccttatgg gtgcgaattc tgtacggtta cgggtttctt tggcgactcg attcggttcc
     1201 ggtcgaatca aagcatcgtc gacgaaatgc tcaggatcaa agaccgcgcc cgcggcacgc
     1261 gcggcaaggc cgccgtattc ttcgtcgatg ataacttcgc gatcaacgtc aagcgaacca
     1321 aagcgctgtt gcgcgatatc atcgcggccg gagccaaact ggcgtgggtg gggcagatca
     1381 gtgccaacct gctgcgggat gaagagctgc tcgatctgat cgccgcttcg ggcgggaaat
     1441 ggattttcat cgggatggag tctctcgatc ccgccaatct ggccagcgtc aacaagagct
     1501 tcaacaaacc ggcggattac gaccgcgtgc ttcaggctct ggccctgcgc cacgtctatg
     1561 cgatcacctc gtttatcttc gggctggaca acgatactcc aggggttgcg gagcgtactc
     1621 tctcggagat ccgcgcgtgg cctccggggc tgcccgtctt tggacagatc acgccctttc
     1681 ccgcaactcc gttgtatgag cggctgaaaa gggaagggcg gctgacacgg ccgaaccact
     1741 ggctcgagtt cgccccgttt cagatggcgc acacgcctct gaagatcacg atttccgccg
     1801 tacagaacga ggttcgatac gcatgggtga actcttacag ccctgctgcg atcgagagag
     1861 ccatcgagtc gatcgtcgac gagccggcag cctacaagat cagccacctg atgtcccggc
     1921 tctttttccg tggaatttat ttcccgcaaa agggagcatg ggactggttc aagctggcgg
     1981 ttcagaaccg gcgcgcgatc agtcgcgtga tcagggactc attaacccgc tggcacggcg
     2041 accaggaccc gacgcgtcgt ctcgacttca acggctcgcc ggaatcgcct ttccgcgacg
     2101 ctgcggagca gagtgccgga gcggactgag tttttccgga ttgtgcgctc ggcaccccac
     2161 ccttcccata ctgtgccgcc ggcgcgcgcc atcggtaagt gtttcggcca ccgctcccga
     2221 gagtcaccgc atcccgccgg aggggcccgc ggtgcagaaa cggcgttcga ggaagtctcg
     2281 tcaaatcggt caggtgaagt cacgtcaagt tgacgagaaa cgcgctagtc gccgaagtgc
     2341 cctgttggcc ttcgggcaga tgatcggcga ctcggacggc ggagcaatat ctcttgacgg
     2401 cctaatctaa aaccgggcaa tctgtagtgg tacggacgta agtgctgact ggtcaggtcg
     2461 accaacgaag gagggaatga tgaccgtgct ccgcaatccc gatcatgtaa ccatcgcggt
     2521 ggcgaacgct gcggccgcga tcgaattctt cgaggtcctg ggcttccgga agcaacatgt
     2581 cgcgatgatc gatggtggtc cacctgcgcg atacatgggc atgccggata tgaaggctca
     2641 gcacatcacg cttgcactcg aaggagccga gcctcgcttc gagatccagt tgctcgagtt
     2701 cgatccaagg ccgccggacg atttaggtga gcacccgaca aatctgcggc gacggggcta
     2761 caatcacctt gcattccgcg tcgacgatat cggcgcggcc accagccacc tggtggccac
     2821 cggagtgaag atgctcagcg atgagatgga ctacatcagc cggcgggtcc ggttcttcga
     2881 aggtcccgag gcgatcacgc ttgagctggt gcaatgggtc gactcagaca gcggcaaggg
     2941 gtgaggacca aacaacctcc gtcatcacag gtacgagcat tgggaccgga cacctgacgg
     3001 cagagacact ggatcgtgaa ggtcataccg tatacgcgac catgcgcgac cggcacggtc
     3061 gcaaccggga ggccggtgat gacgcctcgc caaagagcag agtctcgcga tccgagaaat
     3121 tgatatggat gtgaaggacg aatcggcgat tcaggacgca gtcggtgaga gcgggcgatg
     3181 agagccagca ccgccgcaac tcaggcggac tcgaaaatgc caccgcggtt tggctcgggg
     3241 cccgaccgtg tgaaacgaat aagcagtaat ctgagacagt actaatccct gcgcgggcgg
     3301 agtgcggtgc cgctccgact ggatggtcgg cgatccgtac agaggtcagg cgcggcttcg
     3361 gtgatggcat tctccgagtc gcgcgtcatc cacctgagag cgccttcgaa gaggtag
//
LOCUS       DATAQL010000511         4573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4630466, whole genome shotgun sequence.
ACCESSION   DATAQL010000511 DATAQL010000000
VERSION     DATAQL010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4630466"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="VHS97_08795"
     CDS             complement(<1..1634)
                     /locus_tag="VHS97_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3448337.1"
                     /translation="MSLRILLFMLPFALTSLVRADGPGDNALENVRPVPPKGISLADA
                     DRSALHAGVADLGQEIDALRDALKAKPDLLALLPDIQIYQNAVRYALAHNEFHDIKEV
                     PVAKKLLEQGLERARQLRAGKAPWDAATGLVVRGYVSRIDGSVQPYGLVVPASYQANT
                     PHRFRLDVWCHGRGEKLSELSFINGRQTSPGEFTPKDTFVVHPYGRYCNANKFAGEID
                     LFEAIEDIKKHYPIDEDRLVMRGFSMGGAACWQFAVHYPSVWAAAAPGAGFAETAEFL
                     KVFQSESVQPTWYEKKLWHLYDCTDYAVNLFNCPTVAYSGEVDRQKQAADMMAQALAR
                     EGIELVHVIGAKAGHKYTPEAKVEINRRIDRIVNVGRDPVPVKVRFATWTLRYNRSFW
                     VQVDGLERHWEQARVSADLFGRDGLGPEVITTNVSALTLTMPPGHYPPDLDTKPSMTI
                     DGEKVDATGALSDRSWTAHFRKVESRWRPVDSDDDGQIRKRHGLQGPIDDAFMDSFLM
                     VRPSGTAFHEKVGKWTAAELEHAAEHWRRQFRGEAPV"
     gene            complement(1658..1846)
                     /locus_tag="VHS97_08800"
     CDS             complement(1658..1846)
                     /locus_tag="VHS97_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448338.1"
                     /translation="MAHHRLGEYDEAVRWLGHFRDYRPTQARQNYWVEQEIRLLRREA
                     ERLVLDPAFPADPIAPKQ"
     gene            complement(1858..2463)
                     /locus_tag="VHS97_08805"
     CDS             complement(1858..2463)
                     /locus_tag="VHS97_08805"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3448339.1"
                     /translation="MIIDVSSADVVTVYRALVGVVTPRPIAWVTTVDGQGRVNLAPFS
                     FFNAFGANPPVVVFSPTLRRDGTRKDTLKNLDAVGEFVVNAAVESLAEQVNATSKELP
                     PGQSEADFAGLTLEQSSKVRPPRVAESPVHLECRVCQIMSIGDGPVAANLVIGEVLLI
                     HVADSVLDPGGAVDPKKLRTIARLGGDFYCRSTELFEMVRP"
     gene            complement(2464..3633)
                     /locus_tag="VHS97_08810"
     CDS             complement(2464..3633)
                     /locus_tag="VHS97_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX3448340.1"
                     /translation="MPAYLRKGSIPRKRHIAHPKQPGFKGEGIYYEEVIGLAGFSRAY
                     SIAYHLRPPTRVIRLEPAGTIPANFGEQPSLRHHLVKTRTLPRSGDPITGRVPLFGND
                     DVELARCRPEKPQEELFRNADADEVIFVHSGRGTLHTMFGPLPFRDFDYIVIPRCTTY
                     RLEFEPACQPDLLLIEAAGNISIPGKYLNADGQLKLGAPYSERDLHGPTEVAPIDLEQ
                     ETTVLIKDGRRLTHYTLASHPFDVVGWDGMVYPYTFNADDFEPITGRIHQPPPVQQTF
                     DAPGFVVCTFAPRMLDTDPEAIKVPYAHSNVQSDEVLYYVRGRFGSRRGVEEASFTLH
                     PRGIPHGPHPGTIVASRDVRSTDELAVMIDTFKPLQLTRQAIQLDDPDYPLSWID"
     gene            complement(3668..4381)
                     /locus_tag="VHS97_08815"
     CDS             complement(3668..4381)
                     /locus_tag="VHS97_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3448341.1"
                     /translation="MRQPVPNVASIRDFYAFEQHVATCRRHRGLEMVPQWYEVPVFYF
                     SNPAGVVHHEAEIWAPRGSEALDYELELACVIGREVRDLPDDDRALDAVAGFTIMNDW
                     SARDLQRVEMAVGLGPSKGKDFATSLGPELVSFDELADCYRDGRLHLEMTATVNGRLL
                     SRGNSGSMYWAWPRLLAHASRDTTLRPGDVLGSGTVGSGCILELTPEAAGGWLKPGDL
                     VELTIERLGTLRNRVIHHP"
BASE COUNT          899 a         1391 c         1401 g          882 t
ORIGIN      
        1 acgggtgcct caccccggaa ttgtcgtcgc caatgttcgg cggcgtgctc gagctcggcg
       61 gctgtccatt tgccgacctt ttcatggaac gcggtcccgc tgggcctgac catcaaaaag
      121 ctgtccatga aagcatcatc aatcggccct tgcaatccat gccgcttgcg aatttgaccg
      181 tcatcgtcgg aatcaaccgg ccgccagcgg ctctcgactt tgcggaaatg cgccgtccac
      241 gaccggtcgg agagtgctcc cgtggcatct accttttctc cgtcgatcgt catcgaaggc
      301 ttggtatcga ggtccggcgg gtaatggccg ggaggcatag tcagggtcaa ggcggagacg
      361 ttcgtggtga tgacctcggg gccaagacca tctctgccga agagatcggc gctcacgcgc
      421 gcctgctccc agtgccgttc caggccgtca acttgaaccc agaacgagcg gttgtagcgg
      481 agcgtccagg ttgcgaaccg aaccttgacg ggcactgggt cacggcccac attcacaatt
      541 ctgtcgatac gtcgattgat ctcgaccttg gcctcgggcg tgtacttgtg gccggccttg
      601 gctccgatca cgtggactaa ctcgatgcct tcgcgagcga gcgcctgtgc catcatgtcg
      661 gcggcctgct tctggcgatc gacctcgccg ctgtacgcca cggtcgggca attgaacagg
      721 ttgacggcgt aatcagtgca atcataaaga tgccataact ttttctcata ccacgtaggt
      781 tgaaccgact cgctctgaaa caccttcaag aattcggccg tctcggcaaa gccagcgccc
      841 ggagccgcgg cggcccagac gctggggtaa tggacggcaa actgccagca cgccgcccct
      901 cccatcgaaa agcccctcat caccaggcga tcttcgtcga tcggataatg tttcttgata
      961 tcctcgatcg cctcgaagag gtcgatttcg ccggcgaact tgttggcgtt gcaatagcgg
     1021 ccgtatgggt gcacgacgaa cgtgtccttc ggcgtgaatt cgcccggcga ggtctgtcgg
     1081 ccgttgatga agctcagctc gctcagcttc tcgccccggc cgtggcacca aacatccagc
     1141 cggaaacgat gcggtgtgtt cgcctggtac gaggccggca caaccagtcc gtacggctga
     1201 accgacccat cgatccggga gacatacccc cgcacgacca agccggtcgc ggcgtcccac
     1261 ggcgccttgc cagcgcgcaa ttgccgcgcg cgctcgaggc cttgctccag cagctttttg
     1321 gctaccggaa cctccttgat gtcatgaaac tcgttatggg ccagagcgta gcgcactgca
     1381 ttctgataga tctggatgtc gggtagtaac gccagcagat caggctttgc tttgagcgca
     1441 tcccgcaagg catcaatctc ctggcccaag tcggcgacgc ccgcatggag cgccgagcga
     1501 tcggcgtccg caagtgagat ccccttgggc ggcacgggcc ggacgttctc gagcgcgtta
     1561 tcgccgggac catccgccct gaccagcgat gtgagggcaa atggcagcat gaacagtaga
     1621 attcgtagtg acatagctct ttatctccaa ttgcatacta ttgcttcggg gcaatcgggt
     1681 cggccggaaa tgccgggtcc agaacgagcc tctccgcttc gcggcggagc agacgaattt
     1741 cctgttccac ccaataattc tggcgggcct gggtcggccg ataatctcgg aagtgaccca
     1801 gccagcggac ggcttcatcg tactcaccga gtcgatgatg ggccatggcc gggctcttca
     1861 gggacgtacc atctcgaaga gctcagtgct tcggcagtaa aaatcgcccc ccaatcgagc
     1921 gattgtgcgt agctttttgg gatcgaccgc accaccaggg tcgagcacgg aatccgcgac
     1981 gtggatcaag agcacctcgc cgatcacaag gttggccgcg accggaccgt cgccgatcga
     2041 catgatctga cacacgcggc actccagatg gaccggcgac tcggccacgc gcgggggacg
     2101 tactttggaa gattgctcca acgtcaggcc ggcgaagtcg gcctcgctct ggcccggcgg
     2161 cagttctttg gatgtcgcgt tcacctgttc ggccagcgat tcgaccgccg cattgaccac
     2221 gaattcgccg actgcatcga ggtttttcag cgtgtctttc ctggtcccat cgcggcgcag
     2281 ggtcggcgag aaaacaacga caggcgggtt ggcgccaaac gcgttgaaaa aactgaacgg
     2341 ggcaaggtta acgcgaccct ggccgtcgac ggtggtgacc caggcgatcg gtcgaggggt
     2401 caccacgccc accaaggcgc ggtagaccgt gacgacgtcg gctgacgaca catcaataat
     2461 cattcaatcg atccagctca agggatagtc agggtcgtca agctgaatgg cctgacgcgt
     2521 taactgcagc ggcttgaagg tatcgatcat cactgccagc tcgtcagtcg agcgaacgtc
     2581 gcgactggcc acgatcgtcc cggggtgcgg gccgtgggga ataccgcggg ggtgcaacgt
     2641 gaatgaagcc tcctcgaccc cgcgccggct gccgaatcgt ccccggacat aatacagtac
     2701 ctcgtcggat tgcacgttcg agtgtgcata agggactttg atcgcctcgg ggtcggtatc
     2761 gagcattctc ggggcaaacg tgcaaaccac aaaaccaggg gcgtcgaaag tctgctgtac
     2821 cggcggcggc tggtggatcc taccggtgat cggctcgaaa tcgtccgcgt tgaaggtata
     2881 ggggtagacc atcccgtccc aaccgaccac gtcgaatgga tggctcgcga gggtgtaatg
     2941 cgtgagtcgc cggccatcct tgatgagaac cgtggtttcc tgttcgagat cgatgggcgc
     3001 gacctcggtg gggccgtgca aatcgcgttc gctgtaagga gcccccagtt tcaactggcc
     3061 gtcggcgttt aaatacttgc cggggatcga aatattaccg gccgcctcga tcaagagcaa
     3121 atcgggctgg caggctggtt cgaattccag gcgataggtg gtgcagcggg gaatcacgat
     3181 gtaatcgaag tcgcgaaacg gcagcggacc gaacatcgtg tgtaaggtgc cacggccgga
     3241 gtggacaaag atcacctcat cggcatcggc attgcgaaat agctcttcct gcggcttctc
     3301 ggggcggcag cgggccagct cgacgtcgtc gttgccaaac agcggcacgc gccccgtgat
     3361 cgggtctccc gaccgtggca gcgttcgggt cttgacgaga tggtgtcgca gcgagggctg
     3421 ctcaccgaaa ttcgccggaa tcgtacccgc aggctcgagc cggatcacgc gggtcggcgg
     3481 ccgcagatga taggcgatac tgtaggcgcg agaaaagcca gcaagcccga tgacttcctc
     3541 gtagtagatc ccttcaccct tgaatccagg ctgcttagga tgggcaatgt gccgcttgcg
     3601 ggggattgag ccctttcgca gataggccgg catcgcttgt tctcccagac attggtaggt
     3661 aatccgctca cggatggtgg atgacgcggt tgcgcaacgt gcccagtcgt tcgatggtca
     3721 attcaacgag gtcaccgggc ttgagccagc cgccggcggc ttcgggtgtc agctcgagaa
     3781 tgcaccccga gccgaccgtg ccggacccca ggacatcgcc gggtcgcaag gtggtgtcgc
     3841 ggctcgcgtg cgccagtaat cgaggccacg cccagtacat ggaacccgag tttccacgtg
     3901 ataacaagcg tccattgacc gtagcggtca tttcgaggtg taaccggccg tcgcgatagc
     3961 aatcggcaag ctcgtcaaag ctcactaact caggcccgag cgacgtcgcg aagtctttcc
     4021 ctttgctggg ccccaagccg acggccatct cgactcgctg caggtcgcgc gcgctccagt
     4081 cgttcatgat ggtgaaacct gccacggcat cgagcgcgcg atcgtcatcc ggcaagtcgc
     4141 gaacctcccg gccgatcacg cacgcgagct cgagctcgta gtcaagcgct tcgctcccgc
     4201 gaggcgccca gatttctgct tcgtggtgaa ccacgcccgc cgggttcgag aaataaaata
     4261 ccggcacctc gtaccactgg ggcaccatct cgagcccgcg gtggcggcgg caggtcgcca
     4321 cgtgctgctc gaaggcgtag aagtcgcgga tgctagccac attgggaacg ggctgtctca
     4381 tgagcgcgtg ctctcggtga acggaacggg agaaggccgg ccattctgat caaagcgttc
     4441 ctcgccgcgc ctgctcgaat tcgatcgctt cgaagagcgc cttgaaattc cccttgccaa
     4501 agctgcgtgc cccccggcgc tgaatgatct cgaaaaacag agtggggcga tcctcgacgg
     4561 gtttggtgaa gat
//
LOCUS       DATAQL010000512         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3868393, whole genome shotgun sequence.
ACCESSION   DATAQL010000512 DATAQL010000000
VERSION     DATAQL010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3868393"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..725)
                     /locus_tag="VHS97_08820"
     CDS             complement(360..725)
                     /locus_tag="VHS97_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448342.1"
                     /translation="MLRSDADALARGTVSWQARDDAIDVDSFIAAPDSPLPSNRSRDL
                     RLQWIHFWGRAHMNGLMTGPRGASSVASVQFWEKLRPGRIEPVDLILKPDRDVLNVGA
                     DLGWLGRKPRNARSLRVRS"
     gene            complement(810..>2482)
                     /locus_tag="VHS97_08825"
     CDS             complement(810..>2482)
                     /locus_tag="VHS97_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448343.1"
                     /translation="SLEQTALFSCSAASLTLRNCSITILNNRRAASFAVVRTEPGLSR
                     STRIRFESTLVRGLFSSAFELAGGTELALKRSVVLGGPGPLIRVTDGGTPAEQRLFLF
                     DSILAGPGPLIQRGSKDPGRETRPLMIRAFGTVFGRFHGQGVASVISSACAISQAAKQ
                     IDWGGDNNLFAGWKGFFASGNDPTVTITELAEVRSTWNGADRNSHEIFPSWQPPSDLA
                     ATTPAELKFFVPGREAILRQVAQPRAGLFQKAVGEYVTPTIPQPIGWRSAGPGTSGKT
                     STGQVMRTPPAPERGEPGRPTARSVSTFDITPSSAVKELIFNTENRPWAGDLGAFLRD
                     RLTPAMRNVRVTVAGSGPHRFTPVRLHQGLWLEIRVEPLVSAEPPSWSPQSDATGPAL
                     IELRGGALILSNFVLRHEETSRLEHLIHVEDAHLVLSRCQFVAPGSSADFPCDLIAFT
                     AASTRPYPNDPNRLLFSFPVDRPVCRMDESVLITGGTALRAELGRGMIALTQCAFAAG
                     TSAVELLPSRVSRQRFEADLVINRCTMTSERSVIRLGPWLGLPPGPGL"
BASE COUNT          545 a          735 c          758 g          444 t
ORIGIN      
        1 ccctggccac cgtgatgccc acccccgtgg tgccagccat gccacgacga cccgtgcatt
       61 ccaccaccat gctgtgcttg taggcgactc ggcatgaatg aaagagctat aaccgccacg
      121 acgacgcact tgtggccaga catcttggtc gaagcctccc tgaaacgact ctgaaacagc
      181 caattgaatt atcacactcg agtgggtcca gaatcaatgg atgcactagg cattcgcgtc
      241 tccaaaagcg tcgggtcgat cgcgcgaact cgtcacccgg cctagcggga aaggcagcaa
      301 ggcatcggct gcgagcggtt ctcgccgcac tagaggaacg cgcctaaatc gctcggctgt
      361 caactgcgaa ctcggaggga acgagcgttg cgaggtttgc gtcctaacca tcccaggtcg
      421 gcgccaacgt tcagcacgtc acggtctggc ttgagaatca ggtcaacggg ctcgattcga
      481 ccaggacgga gcttttccca gaactgaacg gatgccacac tgctcgcgcc tcgcggccca
      541 gtcataagtc cgttcatgtg tgcccttccc cagaagtgaa tccactgaag ccgaaggtct
      601 cgtgatcggt tcgacggcaa tggtgaatca ggggcggcga tgaaggaatc gacgtcgatg
      661 gcatcatccc tggcctgcca ggaaaccgtg ccgcgcgcga gggcgtcggc gtccgagcga
      721 agcaagaccg tctccctgga tttccgatca aacgtggcga gaaaggcaca attctgcgat
      781 gtgatcaacc acgggcgatc cggcccgggc tacaagcccg gcccaggcgg caggccaagc
      841 cagggcccga gccggatgac ggagcgctcc gaggtcattg tgcaacggtt tatgaccaga
      901 tcggcttcga atcgttgtcg cgatactcgg ctaggcaaaa gctcgacggc gctcgtgccg
      961 gccgcaaagg cgcactgtgt cagtgcaatc attcctcgtc caagttccgc tcttaatgcc
     1021 gtaccgccag tgatcagaac cgactcatcc attcgacaaa cggggcgatc cacaggaaat
     1081 gagaataaca gcctgttcgg atcgtttgga taaggccggg tagacgctgc ggtaaaggcg
     1141 atcaggtcgc agggaaagtc agcggaagat cccggggcca cgaattgaca gcgagagaga
     1201 acgaggtgcg cgtcttcgac gtgaatcagg tgttcaagtc gcgaggtttc ttcatggcgg
     1261 aggacaaagt ttgacagaat cagtgcccct ccccttagct cgatgagcgc cgggccggtc
     1321 gcatcggact ggggtgacca cgatggaggc tcggcggaga cgagcggctc gacgcggatc
     1381 tcaagccata gtccctgatg caaccgtacg ggcgtgaaac gatgaggccc tgacccagcc
     1441 acagtgactc gaacgtttct cattgctggc gtcaatcgat cgcgcaagaa cgcacccaga
     1501 tcaccggccc atggtcgatt ctcggtatta aatatcagct ctttgacggc ggatgacggt
     1561 gtgatatcaa aggtggagac agaacgcgcg gtcgggcgac cgggctcgcc acgttcgggc
     1621 gccggcggtg tgcgcatcac ctgtcccgtg gaagtctttc cagacgtacc gggccccgcc
     1681 gacctccagc cgatcggctg gggaatcgtc ggagtcacgt attcgccgac ggctttctga
     1741 aaaagtccgg cgcgcggctg ggcgacttgc cgcaagatag cttctcgacc aggaacaaag
     1801 aacttgagct cggcgggcgt cgtggccgcc agatcggagg gcggctgcca ggacgggaag
     1861 atctcgtgac tgttccggtc ggcgccgttc cacgtggaac gaacctccgc cagctccgta
     1921 atcgtgaccg ttgggtcgtt gccggaggcg aagaagcctt tccagcccgc gaagagattg
     1981 ttgtcccctc cccaatcaat ctgtttcgcc gcttgtgaaa tcgcgcaggc agaagaaatg
     2041 acgctagcga cgccttgacc gtgaaatctc ccaaagaccg taccgaacgc tcggatcatc
     2101 agaggccgtg tctcgcgacc gggatccttg gatcctcgct ggatgagggg tccagggcca
     2161 gcgaggatgc tgtcgaaaag gaacaaacgt tgttccgctg gcgtaccgcc atccgtgact
     2221 cgaatcaacg gtcctggacc tcccagcacc accgaacgtt tcagagcaag ctcggtaccc
     2281 cctgccagtt caaaggccga cgaaaacaaa ccgcgcacca gggtgctctc gaatcgtatg
     2341 cgagtcgaac gcgataggcc gggttccgtg cgaacgaccg cgaacgaagc ggcgcggcga
     2401 ttgttgagga tcgtgataga gcagtttctg agtgtcagac tggccgccga gcacgagaat
     2461 aatgcggtct gctcgagcga ca
//
LOCUS       DATAQL010000513         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4173152, whole genome shotgun sequence.
ACCESSION   DATAQL010000513 DATAQL010000000
VERSION     DATAQL010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4173152"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..911
                     /locus_tag="VHS97_08830"
     CDS             144..911
                     /locus_tag="VHS97_08830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3448344.1"
                     /translation="MPEPRLSVLVVAKNEGQNLADCLAAAGWAFERVVVVDAASRDAT
                     LAIARQHADVVAVRQFDNFASQRNFALGLASGDWVLSVDADERVTPSLAAEIDRVMAD
                     PMNLYRGFRVPIRSVILGREFGFSGTQHDQPLRLFRRDSGSWTGLVHETVDLKGRCGS
                     LQNALGHHTIPTVQVFLSKLDHYTTLEAAGLAESNRRFRLSDLALRPVWTFFKLYLFK
                     QGFRDGLEGLMFCALSGVSVAVRAWKHRELNLAGRAS"
     gene            908..1606
                     /locus_tag="VHS97_08835"
     CDS             908..1606
                     /locus_tag="VHS97_08835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3448345.1"
                     /translation="MIADHEAVVASRFDALHGRFKRELGQDDPRLQGIVDSFTPIAGR
                     RVLDLGCGKGRFSKALAERGATVVGLDLSAAMLLGATHIDRVRASARRLPFGPASFDG
                     VIAVEVFEHLAPASLDLVCGEIRRVLSPGGKLVIVDKNARSLNAKRPWLPSVALKWID
                     ERRGLWMYSHREKVRERWFTAGELRGRLGRWFSAIRVEYLLSPVEQGRFPFEYLPSSR
                     LFVLWSAQVPGGDA"
     gene            1603..3099
                     /locus_tag="VHS97_08840"
     CDS             1603..3099
                     /locus_tag="VHS97_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448346.1"
                     /translation="MTELHSPILASLPLLLWKAPPGLELILAQEGVPFETVRDPHPLS
                     FRGGRFVLYDGRAVSAGVLPRLVGADHVTIDIDALRRGEPVDPFEALVDHTAATGSWS
                     VGPWTLSERVARSPKAWIRQRLIGKLREAVTRAGGVWIRLASFPYPYRSAFSFRADLD
                     EPVPEDYHRFAAARAPLDGCCTHFVSTDAYTHHLSVLEDLRRHDTQSHGHFHHVYREP
                     EANRVNLERAHRILWSSGFEPVGFAAPHGRWRPSLDDQLEDLGYLYSSEFQLGHDDLP
                     FFPWKRDRFSRILQIPVHPVCEGLFLEAGVQDSEIIADYFRQVVAAKLDAGELAVIYG
                     HPERRLGRMPAVMAALAETTLGRPLLWRATFSELARWWRWRAERRWLAFQRDDHRLEI
                     QFDEWDAEYAFALEIHRGRFRCSVPVTGPRMSLGLAGLVYERGETSATGVDAPTFSRR
                     GINLKRAVRTAIDWETVTPLEQIPRSSVSNRLKRGLRWWKHTRPGVTT"
     gene            3096..>4106
                     /locus_tag="VHS97_08845"
     CDS             3096..>4106
                     /locus_tag="VHS97_08845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3448347.1"
                     /translation="MTVASSTSSKAAISIESWLATRGGGGPCTPGAVILHAPSFAFQA
                     PGGGENQLIQTGVHLEEMGVPVRLFLPWTERLERARILHLFGMSREGLELARIARARR
                     VPVVLSPICWYEPRALAALEIDPTRKLMALAAWGLRSLVPAVPSWRRALLKLADMVLP
                     NSRSEATQLIRLFGVARERIRVVPNGVLPAMALASPELFRAEWGADPFVLFVGRVEPR
                     KNTLGLILAVRKTGLPLVVIGEAPPGCEPYHEECRRAGGDCVSWLGRLDHHHPLLASA
                     YAAARVFALPSWFETPGLAALEAGLAGCSIAITPYGATKDYFGDLVSYARPDRPAEIG
                     RAV"
BASE COUNT          620 a         1236 c         1384 g          866 t
ORIGIN      
        1 tcgactactc ggccatgccg acctgcggtg gcatctgtgg attgtactgg cacttgtggg
       61 ccggctcgac gtcgcactgg ccgcctatgc cggttacttc gtgttgcggt ctttggccgg
      121 cgtgatcagg aagggggttc ggtatgcctg agccccgctt atctgtcctg gtcgtcgcga
      181 agaacgaggg gcagaacctc gcggactgct tggcggccgc cggctgggcc tttgaacgtg
      241 tggttgtcgt cgacgcagcc agtcgtgacg cgaccctggc aatcgcccgg caacatgcgg
      301 acgtggtggc ggttcgtcag tttgacaact tcgccagtca gcgtaacttc gccctcgggc
      361 tggcttcagg agattgggtg ctttccgtcg atgccgatga gcgagtgaca ccctctctgg
      421 cggccgagat cgaccgtgtc atggccgatc ccatgaacct ctaccggggg ttccgcgtgc
      481 cgattaggag cgtgatcctg ggccgtgagt tcggcttttc cggcactcag cacgaccagc
      541 ctctgcgcct gttccgccgc gattccggca gttggacggg cctggttcac gagacggtcg
      601 atctgaaggg tcgctgcgga tcgctgcaaa acgcgcttgg gcaccacaca atccccactg
      661 tgcaggtctt tctcagcaaa ctcgaccatt acacgacgct cgaggccgcg ggcctggccg
      721 agtcgaatcg gcgctttcgc ttgagcgatc tggcgcttcg gccggtgtgg acgtttttca
      781 agctctatct gttcaagcaa ggatttcgcg atggccttga agggttgatg ttctgcgctc
      841 tttccggcgt ctcggtggcg gtgcgggcct ggaagcaccg cgagctcaat ctggcgggga
      901 gggcatcatg atcgcggatc acgaagcggt cgttgcgtcc cggttcgacg cgcttcacgg
      961 gcgcttcaag cgcgagctgg ggcaagacga tccccgactg caggggattg ttgactcatt
     1021 cacgccgatc gccggccggc gcgtgcttga ccttggttgc ggcaaaggcc ggttttccaa
     1081 ggcgctggcc gagcggggcg ctaccgtcgt cggactcgac ctgtcggcgg cgatgctttt
     1141 aggggcgacg cacatcgatc gcgtccgtgc ctcggcccgg aggttgccgt ttggtccggc
     1201 gagctttgac ggggtcattg ccgtcgaggt ctttgaacac ctggcgcccg catcgctcga
     1261 ccttgtgtgc ggcgaaatcc ggcgtgtgct cagtccggga ggcaagctgg tcatcgtcga
     1321 caagaacgcg cgctcattga atgcaaagcg gccgtggctg ccgagcgtgg ccctgaaatg
     1381 gatcgacgag cgccgcgggc tctggatgta ttcccatcgg gaaaaagtgc gcgagcggtg
     1441 gttcacggca ggagaattgc gcgggcggct gggaaggtgg ttttcggcga tccgtgtcga
     1501 atacctgttg tcgccggtcg agcagggacg gtttccgttc gagtatctgc ccagctcgag
     1561 gcttttcgta ctgtggtcgg ctcaggtgcc gggaggcgac gcatgacaga actgcattcg
     1621 ccaatacttg cgtcgctgcc acttctcttg tggaaggctc cgcctggact ggagttgatt
     1681 cttgcacagg aaggcgtgcc gtttgagacg gtccgggacc cgcatcccct ctcgtttcgc
     1741 gggggtcggt tcgtcctgta cgacggacgc gcggtttccg cgggagtgct ccccaggctc
     1801 gtgggcgcgg atcacgtgac gatcgatatc gatgccttga ggcgcgggga gccagtggac
     1861 ccgttcgagg ccttggtcga ccacaccgcg gcaacgggtt catggtcagt cggaccttgg
     1921 acgctttccg agcgggtggc gcgatcacca aaagcctgga ttcgccaacg attgatcggc
     1981 aaactgcgcg aggccgtgac tcgtgcaggg ggtgtgtgga tcaggctggc gtcctttccg
     2041 tatccttatc gctcggcctt cagctttcgt gccgacctcg acgagcccgt gccggaagac
     2101 taccaccgct ttgccgcggc ccgtgctccg ctcgacgggt gttgcacgca ctttgtgagc
     2161 acggatgctt atacgcacca tctgtcggtc ctcgaagatc tgcggcggca cgatacacaa
     2221 tcgcacggtc acttccacca tgtttatcgt gagcccgaag ccaatcgcgt caacctcgag
     2281 cgtgcccatc gcattctctg gagctcggga tttgagcctg tcggcttcgc ggcaccgcac
     2341 ggccgctggc gcccgagcct ggacgatcag cttgaagatc tgggctatct ttattcgtcc
     2401 gaatttcaac ttggccacga cgacttgccg ttctttccct ggaaacggga ccgtttctca
     2461 cgaatcctcc agatcccggt ccaccccgtc tgcgaggggc tgttcctcga ggcgggtgtc
     2521 caggattcgg aaatcatcgc cgactatttc cgccaggttg ttgccgccaa gcttgacgcg
     2581 ggtgagctgg ccgtcattta tggccatccc gagcgcaggc tgggtcggat gccggctgtg
     2641 atggccgccc tggcggaaac gactctaggg cggcctctgc tgtggcgcgc gacgttttcc
     2701 gagctggccc gatggtggcg gtggcgagcc gagcggcgat ggctggcttt ccagcgcgac
     2761 gatcaccggt tggaaatcca gtttgatgag tgggacgccg aatacgcgtt tgctctggag
     2821 atccaccgcg gccggttccg ctgctctgtg ccggtgactg ggccacggat gtcgctcgga
     2881 ctcgctggcc tggtttacga gcgcggcgag acctcggcga cgggtgtgga tgctcccacg
     2941 ttcagtcgac gcggaattaa tttgaaacgt gccgtgcgga ccgcgatcga ttgggagacc
     3001 gtgacgccgc tggaacagat cccgcggtct tcggtctcca atcgactgaa acgcggcctg
     3061 cgctggtgga agcacacgag accgggagtg acgacatgac cgtggcgtcg tcgacttcga
     3121 gcaaagccgc gatctcgatc gaatcgtggc tcgccacgcg cggcggtggc ggcccgtgca
     3181 caccgggggc ggtcatcctc catgcgccgt cgtttgcctt tcaggcgcca ggcgggggcg
     3241 agaaccagct cattcagacc ggcgtgcatc tggaagagat gggcgtgccg gttcgcctgt
     3301 ttttgccgtg gactgaacgc ctcgagagag cgcggatttt gcatctcttt gggatgtcgc
     3361 gcgaggggct cgaactggcg cggatagcgc gggcgcgccg cgtcccggtc gtgctctcgc
     3421 cgatctgctg gtatgaaccg cgcgcgctgg ccgcgctcga aattgatccg actcgaaaac
     3481 tcatggcact cgcggcctgg gggctgcgat cgctggtgcc ggcggtgcca agctggcggc
     3541 gcgcgctctt gaagctggcg gatatggtct tgcccaattc gcgctccgag gcgactcaat
     3601 tgatacgact ctttggcgtt gcccgcgaac gaattcgcgt tgttcccaat ggcgtccttc
     3661 ccgcgatggc gctggctagc cccgagttgt tccgcgcaga gtggggcgcc gatccgttcg
     3721 ttcttttcgt gggacgcgtc gaaccgcgca agaataccct cgggctgatc ctggccgtgc
     3781 gcaaaacggg gctgccgctg gttgtgatcg gtgaggctcc gcccggatgt gagccctatc
     3841 atgaggaatg ccggcgagca ggcggcgatt gcgtttcctg gctcgggcgg ctcgaccatc
     3901 accatccgct cttggcctcg gcctatgccg cagcgcgggt ctttgcgctg ccgagctggt
     3961 tcgagacgcc cggattggct gcgctcgagg cgggtctcgc gggctgttcg atcgcgatca
     4021 cgccttacgg agcgacaaag gactacttcg gcgacctcgt gagctatgcg cgcccggatc
     4081 ggcccgccga gatcgggcga gctgtg
//
LOCUS       DATAQL010000514         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_967178, whole genome shotgun sequence.
ACCESSION   DATAQL010000514 DATAQL010000000
VERSION     DATAQL010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967178"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VHS97_08850"
     CDS             <1..350
                     /locus_tag="VHS97_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3448348.1"
                     /translation="LNVSSFMGVILLIGLDVKNGIILIEYIGQLRAEGMALHEALLKA
                     GRTRFRPILMTSLCTILGLVPLAMGLGPGAQMQQPLAIAVIGGLITNMLLTRLLIPVG
                     YLAFEKIRGWLSF"
     gene            617..2581
                     /locus_tag="VHS97_08855"
     CDS             617..2581
                     /locus_tag="VHS97_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3448349.1"
                     /translation="MSLSSSDQNTESTFDQLNLSGPLLQAIESAGYLAATPIQARAIP
                     LILAGRDVLGQAQTGTGKTAAFAVPMLQGIDLEKRVPQVLVLTPTRELAIQVAEAFKR
                     YAAGLKGLRAVAIYGGQDYHTQFRQLDRGVHVVVGTPGRVMDHMRRGSLKLDGLRGLV
                     LDEADEMLRMGFAEDVDWILTQAPAQRQIALFSATMPESIRRIAQRHLRNPAEITIKQ
                     KAATADTVRQRFVVVGPQQKEEALARILEAESIEAVLVFVNTKNTTEPLAEYLSRRGH
                     RTAALSSDVSQNQRERIVERLRAGKLDVIVATDVAARGLDVQRISHVINYDFPMDSEN
                     YVHRIGRTGRAGRSGQAILFLHPRGRHLLRRIEQATRQTIELMDVPTNQDINQRRVER
                     FHERITASIAHPDLAAFTSLVEQYRANNDVPLDRVAAALAVIAAGDKPLFLTDLPDEV
                     TFTDTREGRDGSRRESMRRTLEAGGRVGPDGRGQRGRGEGRPPERMETYRIAVGRVHQ
                     VKPANIVGAIANETGLESRFIGRIEIFDEHSTVDLLEGMPHKIFHTLKKVKIAGRALD
                     ISRADEAAAPEDWPIDDPDNAPAGAVRAAAGVSEAPDPQEPAGTPESKSPAPKDRPLG
                     KKIKAKSKAKRIITKSKGARSAGKRLLSTR"
     gene            complement(2578..>3265)
                     /locus_tag="VHS97_08860"
     CDS             complement(2578..>3265)
                     /locus_tag="VHS97_08860"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX3448350.1"
                     /translation="RLDCALFFRTGFRYIQREQAASARADTRRDASATTQVGKVEIVG
                     IETLCMRTARLDLVAATVEHLAAELENTQRFGKLLGAVVLPSWPPGEYDRHAISYFHE
                     RLAEGDSSAAGWYVWYAIRRTTADCPATLVAGGGYFGPPLQDGTVEVGYSVVPEWRRH
                     GYATELVQALVQRAFDDPAVRRILAEAEVANVASIGVLKRCGFRRMGTGREPHYDRFQ
                     RDREAAATAL"
BASE COUNT          616 a         1065 c         1004 g          580 t
ORIGIN      
        1 cgctcaacgt ttcgtcattc atgggagtga ttctgctgat cggcctggac gtcaagaacg
       61 gcatcatcct gatcgaatac atcggccagc tccgcgcgga ggggatggcg ctgcacgagg
      121 ccctcttaaa ggctggccgc acgcggtttc ggccgatctt gatgaccagc ctatgcacga
      181 tcctggggct ggtcccgcta gcgatgggct tgggacccgg ggcccagatg cagcagccgc
      241 tggcgatcgc cgtcatcggc ggcctgatca ccaatatgct cttgacccgg cttctgatcc
      301 cggtgggcta cctggccttc gagaagatcc gcggatggct ttctttttag actcacactc
      361 gttacactat gctaatggat gcatttcagg ccagcttcag attgtcccgc ctgcgcgccg
      421 tttgttgatc tctccgcggt ttctgaatcc tacctgaggc gttcgatcca cctgttcatg
      481 ggcccgctgt gaagttcgca gcgctatggt attcgccgct cgtcggcgat gtgccggggc
      541 tcgctgtcgg tgaagtgttc cgccgtctgc cgtgcggtga acaggtcata cttcctcgct
      601 ctcaggtaca tgctgaatgt ctctttcctc gtccgatcag aataccgaat ccacgttcga
      661 ccaactcaac ttgagcggac ctttgcttca ggcgatcgag agcgccggct atctggcggc
      721 gacgccgatc caggcccgcg cgattccgct catattagcc ggccgcgatg tgctcggtca
      781 ggcgcaaaca ggaacgggga agaccgcggc gttcgccgtc cccatgctcc aggggatcga
      841 cctggagaaa agagtgccgc aggtccttgt gctgacgccg acccgggagc tcgcgatcca
      901 ggttgctgaa gcgttcaaac gctatgccgc cggtctcaag gggctgcggg ccgtcgcgat
      961 ttacggcggc caggactatc acacccagtt ccgccagctc gatcgcgggg tgcacgtcgt
     1021 cgtcgggacg ccaggacgag tgatggacca catgcgccgt gggtcgctca agctcgacgg
     1081 attgcgcggg ctggtgctcg acgaggcgga cgaaatgctg cggatgggct tcgccgagga
     1141 cgtggactgg atcctcacgc aggcacctgc ccagcggcag atcgcgctct tttcggccac
     1201 catgcccgag tcgatccgtc gaatcgctca gcggcatctg cgcaaccccg ccgagatcac
     1261 gatcaagcag aaagccgcca ccgccgacac ggttcgccag cgcttcgtcg ttgtcggtcc
     1321 gcagcagaaa gaggaggcac tcgcccgaat actggaagcc gaatcaatcg aggcggtgct
     1381 cgtctttgtc aatacgaaga acacaaccga gccgttggcc gaatacctgt cgcggcgggg
     1441 ccaccggacg gccgctcttt ccagtgatgt ctcgcagaac cagcgtgagc ggatcgtcga
     1501 acgactgcgg gccggcaagc tcgacgtgat cgtcgccacg gatgtggcgg ctcgcggcct
     1561 ggatgtgcag cggatcagcc acgtgatcaa ctacgacttt cccatggaca gcgaaaacta
     1621 cgtgcaccgg atcggccgca cgggccgggc aggccgcagc ggacaggcca tcctcttcct
     1681 gcacccccgc ggccgccacc tgctgcggcg gatcgaacag gcgacccggc agacgatcga
     1741 gctgatggac gtgcccacga atcaggacat caatcagcgc cgcgtggaac gttttcacga
     1801 gcggatcacc gccagcattg ctcatcccga cctggcggcc tttacttctc tggtcgaaca
     1861 atatcgtgcc aacaacgacg tcccgctgga tcgcgttgcg gcggccctgg ctgtcatcgc
     1921 agccggcgac aaaccgcttt tcctcaccga cctacccgac gaggtgacct tcaccgatac
     1981 tcgcgaaggt cgcgacggct cgcggcggga atcgatgcgg cgcacgcttg aggccggcgg
     2041 tcgagtcggc cccgacggcc gaggtcagcg cggtcgaggc gaagggcgcc cgcccgagcg
     2101 gatggaaacg tatcggatcg cggtcgggcg agtccaccag gtcaagccgg cgaacatcgt
     2161 gggcgcgatc gccaacgaga ccggactcga gagccgcttc atcggacgca tcgaaatctt
     2221 cgacgagcac agcacagtcg acttgctcga gggcatgcct cacaagattt ttcacacgct
     2281 gaaaaaggtc aaaatcgccg gccgcgcgct tgatatctcg cgtgccgacg aagccgccgc
     2341 gcccgaagac tggccgattg acgatcctga caatgcccct gccggtgcag tgcgcgccgc
     2401 cgccggggtg tccgaagcac ccgacccgca agagccagcc gggacgcccg agtccaagtc
     2461 ccctgccccc aaggatcggc ctctgggtaa gaagatcaag gccaagtcca aggcaaagcg
     2521 aatcatcacg aagtccaaag gtgcccgctc cgccggcaaa cgattgctgt caacacgtta
     2581 aagggccgtc gctgccgctt cgcgatcgcg ttgaaaccgg tcgtaatggg gctcgcgtcc
     2641 ggttcccatt cttcggaatc cgcagcgctt gagtacacca attgacgcca cgttggcgac
     2701 ctcggcctcg gccaggattc ggcgcacggc cggatcatcg aacgcgcgct gcacgagggc
     2761 ctgtaccagc tcggttgcgt agccatggcg acgccactcg ggcacgaccg agtatccgac
     2821 ctcgaccgtc ccgtcctgga gtggcggacc gaaatagcca ccgcccgcga cgagggtcgc
     2881 tggacagtcc gccgtcgtcc gccgaatggc gtaccatacg taccatccgg cggccgacga
     2941 atccccttcg gcgagccgct cgtggaaata agaaatcgcg tgcctgtcat attcgcccgg
     3001 aggccagctt gggagaacca cggcaccgag aagtttgccg aagcgctggg tgttttcgag
     3061 ctcggccgcg aggtgttcga cagtcgctgc caccagatcc agacgggccg ttcgcatgca
     3121 cagtgtctcg atacccacga tctcaacctt cccgacctgg gtcgtcgcgg aggcgtcccg
     3181 ccgagtgtcg gcgcgtgcac ttgccgcttg ttcacgttga atataacgaa aacccgtccg
     3241 gaaaaagagc gcgcaatcca gacgc
//
LOCUS       DATAQL010000515         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2952247, whole genome shotgun sequence.
ACCESSION   DATAQL010000515 DATAQL010000000
VERSION     DATAQL010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2952247"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VHS97_08865"
     CDS             <1..726
                     /locus_tag="VHS97_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448351.1"
                     /translation="ELQAEHAGKVLTANNDRVVLNVASEGDPLFLTITCRTGSNDRPF
                     SLKATHRAHDEKSDHVLERDRLFVPWSPFPSTVTAVPLVVPDLSGGDPVRGRTIFSGE
                     QGRCAQCHAFRGQWGQVGPDLTEFTKKGRAEIYRAIAEPSASIEPEYTSYSIATKGGQ
                     VVVGVVRAEDARAIRVTDTNAHAVIIARDEIEQIRPSANSIMPVGLTGVLGAGALRDL
                     IAFLVSESTPAEQGQSPRSNAKP"
     gene            916..>2123
                     /locus_tag="VHS97_08870"
     CDS             916..>2123
                     /locus_tag="VHS97_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3448352.1"
                     /translation="MDASHRHSVGFVRREFIQVGFSGLLGLGLSDLLAARARTDTGAH
                     QSVSHSTPARAKSVILVFLTGGLSHLDSFDMKPSAPAGIRGEFQPIATSASGIQFCEH
                     LPQLAARADKLAIVRSLSHKYTNHLNATHEVLTGQSQPGAFFDKIASRDDFPCYASAI
                     DAVRPRADGIPSGVMLPTFLMSGPLIWPGQHGGFLGPKHDPWQVRQDPNRPGFGFEGM
                     SLPAGMSVERLDRRKSLLDQLGSRRDQLTALAAEAKDPFAEQIERAYTLLLSGKVARA
                     FDLDQEDPRLRDRYGRHMYGQSLLLARRLVGAGVPVVQVNMGRVQTWDTHSANFKALK
                     DRLLPPTDQGVAALLDDLETQGMLDETMVVVTGEFGRTPRIGASTGNNNTRDGRDHWA
                     AVFSAAFAGGG"
BASE COUNT          396 a          644 c          662 g          421 t
ORIGIN      
        1 gaacttcagg cggaacatgc gggcaaggtg ttgactgcga acaatgaccg cgtggtgctg
       61 aacgtggctt cggagggaga tcctctcttt ctgacaatca cctgccgaac cggcagcaac
      121 gaccggccgt tttcgctgaa agcgacccat cgtgctcacg acgagaagag cgatcatgtg
      181 ctcgagcgcg accggttgtt tgtcccctgg tcgccctttc cctcgactgt aaccgcggtg
      241 cctttagtcg tgcccgacct gtccggcggc gatcctgtgc gcggtcgaac gatcttttcg
      301 ggtgagcaag gccgctgtgc gcaatgccat gcgttccgag ggcagtgggg ccaggtcggg
      361 cctgacctga ctgagttcac caagaagggc cgggccgaga tttatcgtgc catcgccgaa
      421 ccgagtgcgt cgattgagcc cgagtatact tcctattcga tcgcaaccaa gggaggccag
      481 gtcgtcgtcg gcgtcgtacg agccgaagac gcccgggcga tccgggtcac agacacgaat
      541 gctcatgccg tgataatcgc acgtgacgag atagagcaga tccgccccag tgcgaattcg
      601 atcatgccgg tcggcctgac cggtgtgctc ggcgctggcg ctttgcgcga tctgatcgct
      661 tttctcgtat cggagtcgac gccagcggaa cagggccaaa gccctcgttc gaacgccaag
      721 ccttgaatcg ccgaggggat cggcccccac gtcgtgcggg gcccacggaa ttcacgcgac
      781 gacctcgata caaacctgaa gttaaatgtg cgtgcgactt gcccttcgcc aagtgttcaa
      841 gataaaatca aaacttgtta ttcggcctga acagtgaggc tctgagccaa cgacaaccga
      901 tctggaaagg acacgatgga cgcgagtcac cgtcattccg ttggctttgt ccgccgtgag
      961 ttcatccagg tgggcttttc ggggttgctc ggccttggat tatccgactt actggcggcg
     1021 cgagcgcgta cggacacagg cgcacaccag agtgtttccc attcgactcc cgcgcgtgcc
     1081 aagtcggtga tcctggtctt tctcaccggc ggtctctccc acctcgactc gttcgatatg
     1141 aagccatccg cgccggccgg tatacgtggc gagttccagc caattgccac gagtgcgtcg
     1201 ggcattcagt tctgcgagca cctaccgcaa ctcgccgccc gcgctgacaa gctggcgatt
     1261 gtccggtcgc tgtcgcacaa gtatacgaat cacttgaacg ccacccacga agtcttgacg
     1321 gggcagtcgc agccgggcgc gtttttcgac aagattgcct cgcgcgacga ctttccctgt
     1381 tatgcgtcgg caatcgacgc cgtccggccc cgggcggatg gaatacccag cggggttatg
     1441 ctgccgacgt tcttgatgtc aggtcctttg atctggccgg gccagcatgg gggctttctg
     1501 ggtccgaaac acgatccatg gcaggttcgc caggatccca atcgtcctgg tttcggattt
     1561 gaaggaatgt cgttgccggc ggggatgagt gtcgagcggc tcgatcggcg caaatcgctt
     1621 ctcgaccaac tgggatcgcg gcgcgaccag ctcaccgcac tcgccgcgga agcaaaagac
     1681 ccgttcgccg agcagatcga gcgagcctac acactcttgc tctcgggtaa ggttgcgcgg
     1741 gcgtttgatc ttgaccaaga agatccgcgg ctgcgcgacc gctatggccg gcacatgtac
     1801 ggccagtcgc ttttgctggc caggcgactc gttggggccg gcgtgccggt cgtccaggtc
     1861 aacatgggcc gggtgcagac gtgggataca cactcggcca atttcaaggc actcaaggat
     1921 cggttgctgc cgccgaccga ccagggcgtg gcggcattgc tcgacgatct ggaaacgcag
     1981 ggaatgctcg acgagacgat ggtcgtcgtc accggcgagt tcggccgaac gccccgcatc
     2041 ggcgcgagta cgggaaataa caacacgcgc gatggccgcg atcactgggc ggccgtcttc
     2101 tcggccgctt tcgccggcgg agg
//
LOCUS       DATAQL010000516         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1425481, whole genome shotgun sequence.
ACCESSION   DATAQL010000516 DATAQL010000000
VERSION     DATAQL010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425481"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VHS97_08875"
     CDS             <1..307
                     /locus_tag="VHS97_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX3448353.1"
                     /translation="SRKDGAPLMLAGIWEESEYKGDRRVAFAILTDEPNELVAPYHDR
                     MPLALADDKVATWIDLAVDEPLRGNLLLDLAEFTVRPIDRAMNNARQKDLAAIDPAA"
     gene            544..1680
                     /locus_tag="VHS97_08880"
     CDS             544..1680
                     /locus_tag="VHS97_08880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3448354.1"
                     /translation="MRISLMLAAALALGSAASAAERDRPTAILVSPIHEAQIVRADDG
                     KDHVEYELLVVNVFSEPVTLASVTVLDPTGKELMRIEGGTLAAATQTLFAKTTTPVVP
                     ASAAVSVDVDLILAPDTAPERVTHRIAYALKADSELALMVGSLDVNAPEVAINHQPAI
                     VIRPPREGKGWLATNACCKPNIHRDLRVAVDGVRIETAETFAVDWGKVKNDRIFDGDG
                     TRVEQYYGFGEDVFAVADGTVVYVHDGMPDETPPVLMVPNSKSDYGGNNVILEIAPNV
                     FAWYAHLRQGSLTVKVGDAVKAGAPIAKLGNTGPSEGPHLHFGLFNKSDPIAGRSLPF
                     VFDIFTLGGAVDFDTSKGDRLVIRPDARQVRSAYPLYGGIQDFP"
     gene            2091..>2898
                     /locus_tag="VHS97_08885"
     CDS             2091..>2898
                     /locus_tag="VHS97_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3448355.1"
                     /translation="MAKAENSGVDDGLKKYDVTTNGISLHVTDQGEGPAVLFSHGFPD
                     TSYTWRRQMKAIASAGYRAIAPDMRGYGRSSAPADAVLYTPLQTTGDLVGLLDALKIS
                     SAVLVGHDWGATHAWNAALMRPDRFTAVFCLSVPYVPRGDVSVFERMRESGHQNDFYM
                     FEQIRPDADQIWADAAVTIPGILYWASGSAPADKRWSPMDPARSLHRPAPGPMPSWAA
                     PDYVKHNVAEFQRTGFHRGLNYYRAAEPYFYLSPLAKARSFLLHCGEADGL"
BASE COUNT          571 a          870 c          887 g          570 t
ORIGIN      
        1 ctcgcggaag gacggcgcgc cgctcatgct ggcgggcatc tgggaggaat ccgaatacaa
       61 gggggacagg cgtgtcgcgt ttgctatcct gacggatgag ccgaatgagt tggtggcacc
      121 ctaccacgac cggatgccgc tcgcgctcgc cgacgacaag gtcgcgacct ggatcgactt
      181 ggccgtggat gaaccactcc ggggaaacct cctgctcgat ttggcggaat tcaccgtccg
      241 gccgatagac cgggcgatga acaacgcgag gcagaaggat ctggcggcaa ttgatccggc
      301 ggcatgaact ccgctgcaag aagcgccctt agatcgaaat ccgctcgacc tcgtcgagcg
      361 tgatctggtc gccggtgcga tcgtacagct tggttgtgcg cgggctttga tgcacgacca
      421 tagcctgggc gttctcaagc gtgccgcccg ccttgagata ggccgttatg ccggtcgcgc
      481 ggaaggtaat ggtggcttta aggatcggcg ctgcttcaac acttggaaac cagggaggga
      541 cagatgcgca tttctctcat gttggccgcc gccttggccc tcgggtcagc cgcttcggcc
      601 gcggagcgcg accggccgac tgcaatcctc gtcagtccga tccacgaggc ccagattgtc
      661 cgcgccgacg acggcaagga ccatgtggaa tacgaacttc tcgtcgtcaa cgtgttctcc
      721 gagccggtga cgctggcgag cgttactgtt ctcgatccca ccggaaaaga actcatgcgg
      781 atcgaagggg gcacccttgc ggccgcgacc cagaccctct tcgcgaagac aacaacacca
      841 gtcgttcccg catcggccgc cgtctcggtt gacgtcgatc tgatcctcgc accggacacc
      901 gccccggaac gggtcacgca ccggatcgcc tacgccctca aggcggactc ggagcttgct
      961 ttgatggtcg ggagcctgga tgttaacgca ccagaagttg ccatcaacca ccaaccagcc
     1021 attgtgatca ggccgccccg tgaggggaag ggctggctcg cgaccaacgc ttgctgcaag
     1081 cccaacatcc acagagacct tcgtgttgcg gtagacggcg tccgcatcga gaccgccgag
     1141 accttcgccg tcgactgggg caaggtcaag aacgacagaa tcttcgacgg cgacggcacg
     1201 agggtcgagc agtactatgg ctttggcgag gacgtcttcg cggtcgccga tggcaccgtg
     1261 gtctacgtcc acgacggcat gcccgatgag acgccgcccg tcctgatggt cccaaactcg
     1321 aagtcagact atggcggcaa taacgtgatt ctcgaaatcg ctccgaatgt cttcgcctgg
     1381 tacgcgcatt tgcgccaagg cagcctcacg gtgaaggtcg gtgacgcggt gaaggccggg
     1441 gcaccgatcg ccaagctagg caacaccggc ccctccgaag gcccccacct gcatttcggt
     1501 cttttcaaca agtccgaccc tatcgccgga cggagcctgc ctttcgtctt tgacatcttc
     1561 accttgggcg gcgcggtcga tttcgacacc tcgaaggggg atcgactggt gattcggccg
     1621 gatgcgcgac aggttcggtc cgcctatccg ctctacggcg gcatccagga tttcccgtag
     1681 cgcgatcagc catcacgcga ctccaacacc cgcgtcgagg gcgaaatgtc ggcttcgggt
     1741 caagtttccg cgcggccttt ggtccgatgc gtcaattgcg agaggatcat ctgcgtggca
     1801 atctagttgc cagtttgcac atttgacgcg gcgacgaagg taggtccgcc aaaaaggaaa
     1861 cgacggtacg ccggtaggac gggaacctct gttccgttct ggaaggccaa tgcgacatgg
     1921 tgggaaaaga ccgagatgat cccgaggccc gctttagctg cttcgcaggc agcgtcggtt
     1981 gaattaacgg tcagtcgata gcgaaccgga acctcgacgt tgtcggcaag gctccagaaa
     2041 ctttgctcgt cgctggcact gccgtggtcg caagcctggt ggagggtcaa atggctaaag
     2101 cggaaaattc tggggtcgac gacggcctca agaagtacga cgtcacgact aatggcatct
     2161 ccttgcacgt aactgaccaa ggggaagggc cagccgttct tttcagccac gggtttccgg
     2221 atacttccta tacgtggcgc cgacagatga aagccatcgc gtcagccgga tatcgagcaa
     2281 ttgctccaga catgcggggc tatggacgga gctcagctcc ggcggacgct gttttgtaca
     2341 cgccgctgca aacgaccggc gacctcgttg gtctgctcga tgcgttgaaa atctccagcg
     2401 cggtcctcgt gggccatgac tggggcgcaa ctcatgcgtg gaacgctgcg ttgatgcgtc
     2461 ctgatcgatt tacagcggtg ttctgcctga gtgtgcctta cgttccgcgt ggcgatgtca
     2521 gcgtcttcga gagaatgcgg gagtcgggcc atcaaaacga tttctacatg ttcgaacaga
     2581 tccggccgga cgccgatcaa atttgggccg acgccgcagt caccattcca gggattctgt
     2641 attgggcatc gggttctgct ccagccgaca aacggtggag tcctatggat ccggcacgga
     2701 gccttcatcg cccggctcct gggccgatgc cgtcatgggc ggcgccagat tatgtcaagc
     2761 ataacgtcgc tgaattccag cgtaccggct ttcaccgtgg ccttaactac taccgcgccg
     2821 ccgaaccgta cttctatttg tcgccgcttg caaaggcgcg aagcttcctt ttacattgcg
     2881 gggaagcgga tggcttga
//
LOCUS       DATAQL010000517         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5395607, whole genome shotgun sequence.
ACCESSION   DATAQL010000517 DATAQL010000000
VERSION     DATAQL010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5395607"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..824
                     /gene="msrB"
                     /locus_tag="VHS97_08890"
     CDS             27..824
                     /gene="msrB"
                     /locus_tag="VHS97_08890"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX3448356.1"
                     /translation="MSRFYAQLWVGVPAAAFFCLGCSLGARAQDPLANAPLAPGSAVS
                     SDASDQNKPDSTTKSETKALAKPEPEFVVKTDAEWRKILTRTQYAVTRQKSTEPAFSG
                     KYATGHYQGTFLCVCCDAALFGAEAKFDSGTGWPSFDRPASMKSIKTAWDNSEIEPRV
                     EVMCRRCGAHLGHVFDDGPTLTRLRYCINSAALKLKPTDSQGALKTVSRETPSKSKAK
                     TKQKSKSMQGSAPKGVAPPTSTSPADSEHAPTRDSVTSKGASSSPSS"
     gene            856..2703
                     /locus_tag="VHS97_08895"
     CDS             856..2703
                     /locus_tag="VHS97_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448357.1"
                     /translation="MASRGDWLLVQELLDRGDPEFVDRLREFSDADILRPFAERWYSN
                     PSPAARRLLLAYLESPLNAPRHEPLIKRLFKRAEAAKDDSAMARFLVAFDRSLRRVER
                     QRRHRQAQWVDTRVEAEKLVALWKSQGYDFTNISESLIRRSAGRYYVFAMTTWTEPFL
                     TVPAGTTMPKHDRPLRRKAERFRLFSVPTRHYMRRRAWRYFRRMGKTQPERYVAAISE
                     ALVLYQDADVATGLALLDNWGLVHALFHHSPVLEARPRGWRPAEGHSLGELEPAPIYP
                     HLWPSSPRAIFDLLRYGRCRPVRQWAVKMIKTIDMATIRASVGLEKIFGLLGHGDSEI
                     AIWAIELLGATAELAAIGAARWLSLVETAGPAALERLTELMARHVDPEQLSLADAVRM
                     AASRPLPLARLGLSWLKTKMPQSDVDLWSLFALLEAEAQPLRAEILGWLRAALSASSE
                     FRWEWVLEFLDSRHADARGMGLSWFRTEPRARDEVTLWQRLLESPHDDVRLALVADLE
                     KRLAGASESVDPARSLDPERLRLLWASVLLNVRRGGRAKPGVIGQVALRLERRPDEAE
                     QLLPLLGVALRSVRAPERKAALAALVRMVERRPDAKHLVQTALPELQWA"
     gene            3035..>4008
                     /locus_tag="VHS97_08900"
     CDS             3035..>4008
                     /locus_tag="VHS97_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448358.1"
                     /translation="MNLTLAYRGKSAIVSTTAGLAVALAPNLRRDRVGFDGILRDPLS
                     FREAMGALHDIVVSDLRYKPPDRLGYEVYKAERSRREASLRQAVTKQARAAIYESMPE
                     LPEAYSAELELRYRTLRQQYWKARIQYSDYLCLHDPELWRILVPCDPVITVAPDCLFF
                     ECFSADESSYGCLTVDRGAFEHERDVAAGTTNVDYSWTLYEHFQSMRSYRETRFMVDP
                     AGFEVQTDQSTVVREEKIDLPPSWLRGFLQLQAAMSLPSRRVPIGRGGLYNILAYLKR
                     HRASRSPRAIRFELETGRPPIAVLEPWEERIRVGDEPYAGPRSEIIRV"
BASE COUNT          711 a         1254 c         1272 g          771 t
ORIGIN      
        1 ggtccgaatg gaggagacaa gacagcatgt ctcgatttta cgcgcaattg tgggtgggtg
       61 tgccggcagc cgcgtttttc tgtctcggtt gttcgctcgg cgcccgagcg caagacccgc
      121 tggccaacgc gcctctcgcc ccgggttcgg ctgtctcgag tgatgcgagc gatcagaaca
      181 agccggattc cacgaccaag agcgaaacca aggcactggc aaagcccgag cctgagttcg
      241 tggtcaagac tgatgcggaa tggcggaaaa tcctgacgcg gactcagtat gccgtcacgc
      301 gccaaaagtc gacagagccc gcgttttcgg gaaagtacgc caccggccac taccagggca
      361 cgttcctctg cgtctgctgc gatgcggcgc ttttcggtgc agaagccaaa ttcgattcgg
      421 gcacgggttg gccgagcttt gatcgaccgg ccagtatgaa gtccatcaag accgcatggg
      481 acaacagcga aattgagccg cgggtggaag tgatgtgccg tcgttgcgga gcccacttgg
      541 gccatgtctt cgatgacggc ccgacactta ctcgcctccg ctactgcatc aactcagccg
      601 ccctcaagct caaaccgacc gactctcagg gtgccctgaa aacggtttcc cgcgaaacgc
      661 cctcaaagtc gaaggccaag accaagcaaa aatccaagtc catgcaggga tcggctccca
      721 agggcgtagc gcccccgacc tcgacgagtc cggccgattc cgaacatgct cccacacgag
      781 actcagtaac gagcaagggc gcttcttcgt caccgtcgtc gtgagtccgg ccgcgcccac
      841 ggggaatcta cgagcatggc gagccggggc gattggctct tggtccagga gctgctcgat
      901 cggggcgacc ccgagttcgt cgatcggctg agggaattct ccgacgctga cattctccgg
      961 ccattcgccg agcgctggta ctcaaatcca tcgcccgcgg cgcggcggtt gctgttggcc
     1021 tatttggaaa gcccgctcaa tgcgccgcgg catgagccgc tcatcaaaag gctgttcaag
     1081 agggccgagg ccgctaaaga tgattccgcg atggcgcggt tcctggttgc ctttgatcgg
     1141 tcgctccggc gagtcgagcg acagaggcgc caccgtcaag cacagtgggt cgacacgcga
     1201 gtcgaagccg agaaactggt cgcactctgg aaatcccagg gatacgactt cacgaacatc
     1261 tccgaatcgt taataaggcg cagcgccggc cgttattatg tgttcgcgat gacgacctgg
     1321 accgagccgt tcttgacggt gcctgccgga acgaccatgc caaagcatga ccggccgttg
     1381 cgacgaaagg ccgagcgatt ccgcctgttc tccgtgccca cgcggcacta catgcgccgt
     1441 cgtgcctggc gttattttcg tcgaatgggg aagacacagc cggagcgtta cgtggcggcg
     1501 atctccgagg ccctggtcct gtatcaggat gctgatgtcg cgaccggttt ggcgctactc
     1561 gacaactggg gcctggtgca cgccctgttc catcatagcc cggtgctcga ggcgcggccg
     1621 cgtggctggc ggccggcgga gggccactcg cttggcgagc tcgagccggc accgatctac
     1681 ccacatctct ggccgtcatc gccccgcgcc attttcgacc tcttgcgata cggccgctgc
     1741 cgcccggtcc gccagtgggc cgtgaagatg atcaagacga tcgacatggc tacgatccgc
     1801 gcttccgtag ggctcgagaa gatcttcggg ctgctcggcc atggcgattc cgagatcgcg
     1861 atctgggcca tcgagctcct gggagcgact gcggaactgg ccgcgatcgg cgcggcgcgc
     1921 tggctctcgc ttgtcgagac cgcagggcca gccgccctcg agagattgac cgagcttatg
     1981 gctcgtcacg tcgatcccga gcaattgtcg ttagccgacg cagtgcgtat ggctgcgagt
     2041 cgaccgttgc cgctggcgcg gctcgggctc tcgtggctga agacgaagat gccacagtcc
     2101 gacgtcgatc tttggagtct cttcgcactg ctcgaggccg aggctcaacc gcttcgcgcc
     2161 gaaatacttg gctggctgcg tgcagcgcta tcagcgtcaa gcgagttccg ctgggagtgg
     2221 gtgctcgagt ttctcgacag ccgtcacgcc gacgctcgcg gtatgggtct atcttggttt
     2281 cgcaccgaac cacgggctcg agacgaggtc acgctgtggc agcgcttgct ggaatctccg
     2341 catgacgacg tgaggctcgc tctggtcgcc gatctcgaaa agcgccttgc cggggcgagc
     2401 gagtccgtcg acccggcgcg ctcgctcgat cccgagcggc tccggctgct ctgggcgtca
     2461 gtgcttttga acgtgcggcg cgggggacgg gccaagcccg gcgtgatcgg tcaggtggcg
     2521 ctacggctcg agcgccggcc cgacgaagcc gagcagttgc tgccgctctt aggcgttgcc
     2581 ttgcgttcgg ttcgcgctcc tgagcgcaag gccgcgctgg cggcgctcgt tcggatggtg
     2641 gaacgtcggc ccgacgccaa acatcttgtc cagacggcgc tgcccgagtt gcaatgggct
     2701 tgagcttttg cgatcatgac ccggctgttg cggtgaatga tctggacggc gggcgggctg
     2761 gtgctcgaga ccatcatgcc gcggtgttcc gccgcggtcg aacttgaccc ggagaatgct
     2821 tccccaaggc cgttgttctt gtcggccccg cccgacatct tgtggcggtg ctgattcgac
     2881 gaggtccccg ccgcaagatg tcgggcgggg cctcggtgaa cgaccttggt cgacgcctcg
     2941 aagtcgagtc cggcccgccc gccatccgga tcggaaaaac tggtgcatca gcgcatttgg
     3001 gcacatgctc cagcgcgctc gtcgaggcgg ttcagtgaat cttactctgg cgtatcgagg
     3061 caagagcgcc atcgtctcga ccacggccgg gctggctgtt gcgctggcac caaacttgag
     3121 acgcgatcgc gtcggctttg acggcatact ccgcgatccg ctctcgtttc gcgaggcaat
     3181 gggcgctctg cacgatatcg tcgtgagcga cctgcggtac aagccgcccg atcgattggg
     3241 atacgaggtt tacaaggccg aaagaagccg gcgcgaggca tcgctgcgcc aggccgtcac
     3301 caaacaggct cgcgctgcca tttacgagtc gatgcccgaa cttcctgaag catactcggc
     3361 ggaacttgaa ctacgctatc gaaccctccg ccagcaatac tggaaggcac gcatccagta
     3421 ttccgactac ctgtgcctgc acgaccccga gctgtggagg atcttggtgc cttgcgaccc
     3481 ggtgatcacg gtcgcgccgg attgcctttt tttcgagtgc ttctcggcgg acgagagcag
     3541 ctacggctgt ttgacagtcg accgcggggc attcgagcat gagcgtgacg tcgccgcagg
     3601 cacgacgaac gtcgattact cctggacgtt gtacgagcac ttccaatcga tgcgtagcta
     3661 ccgcgagacg cggttcatgg tcgatcccgc gggtttcgaa gttcagaccg accaatcaac
     3721 cgtcgttcgc gaagagaaga tcgacttgcc gccctcctgg ctccgtgggt ttcttcaact
     3781 tcaagccgcg atgagcctgc cgtcacgccg cgtgccgatc ggtcgcgggg ggctctacaa
     3841 tattcttgcc tatctcaagc gtcaccgcgc ttcccggagc ccgcgcgcga tccgattcga
     3901 gctcgagacc gggcgcccgc cgatcgcggt gctcgagcca tgggaagagc gtatccgagt
     3961 tggtgatgag ccgtacgcgg gaccaaggtc agagatcatt cgcgtttg
//
LOCUS       DATAQL010000518         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2342531, whole genome shotgun sequence.
ACCESSION   DATAQL010000518 DATAQL010000000
VERSION     DATAQL010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342531"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VHS97_08905"
     CDS             complement(<1..323)
                     /locus_tag="VHS97_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448359.1"
                     /translation="MKNLRLFIPLTLAASALHAQPSIKNGGIANVSGYQTTLAPGTVF
                     VIFGSNLGPASIVTATAPAYPTSLGGTSITFTPNSGSAIAAKMVYSLAGQVAGLLPSS
                     AAPGT"
     gene            610..>2439
                     /locus_tag="VHS97_08910"
     CDS             610..>2439
                     /locus_tag="VHS97_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3448360.1"
                     /translation="MTREHWQRVEEIFHAALDRAPEVRRAFLDSACSGDSQLRRLVEL
                     LVAKEQQAGSFLETPAMQDVMVAVAAGDKSLVGLQFGPYSIVSPLGAGGMGEVCRAHD
                     SRLGRDVAIKTLPRAFASDPERLARFRREARTLASLNHPNIAAIYGLEESGDMHCLVL
                     ELVEGETPRGPLTMAAALDCARQIAEALEAAHAKGIIHRDLKPANVKLTPEGRVKVLD
                     FGLAKAIWGEDMDGDLSQRATVAEADTLAGSIVGTPSYMSPEQARGTAVDRRTDVWAF
                     GCLLYELLAGKRAFQSETVSDTIAAVVGSEPDWHALPAKIPGRVRDLLRKCLQKDITR
                     RLPDITDARRTIEKAQRGWNRWQIVAAVTAGLAPLAVGATLWLRGPAPPPNRSDWIQL
                     TKFPDPVSQPAFSPDGRTITFLRSPNTFFAVGQVYVKSLPDGEPQQLTNDGIAKTNPA
                     FSPDGTSIAYTTLDVGFNWDTWVVAAKGGAPRRWRRNVSDLIWTGPREILYSERPSKG
                     IVAAEEDLSGRHNVYLPPNDRGVSRRPHASPDGKWVLLAEISAYGDWEPCRVVPMDGS
                     STGRQVGPPSAACTGGAWSPDGEWVYVTSKAGGLYHIWRQRFPN"
BASE COUNT          495 a          682 c          789 g          473 t
ORIGIN      
        1 taggtgcctg gagcggcgga ggacggcagt agtccagcca cttgtccggc aagcgaatag
       61 accatttttg ccgcgatcgc ggagccggag ttaggggtga aggtgatgga cgtgccgccg
      121 agggacgtgg gataagcggg cgcggtggcg gttacgatcg atgccggccc aaggttggat
      181 ccgaaaatca cgaagaccgt gccgggcgca agagtcgttt gatagccgct gacgttggca
      241 atacctccgt tcttgattga cggctgggca tgcagcgctg aggccgcaag tgtgagtggg
      301 ataaaaaggc gcaagttttt catggttctc aattatgcat tgcgcaaaaa gacaccaaaa
      361 accgtcgttc ctacagtcta aaccttccgt aagtatgtgt tcgcacagca aactcccgtc
      421 gagtcgtcgc aaattccgat aattggaatt atcgttccga tttcgaggtg acgcatatca
      481 tatccgcgat agccgacaag ccagccagat tctgtgacgg ccaggactgg tgtcgaggca
      541 gggcttcgga tccgtcctgg gcacgtctct tcgggcgtta gtgtaataga attgaccgaa
      601 aagctccaca tgacccgcga acattggcag agagtcgaag agatcttcca cgcggctctg
      661 gatcgtgcgc cggaagtccg gcgggcgttt ctcgacagcg cttgcagcgg agacagccaa
      721 ctgcgccgcc tggtggagtt acttgtcgcg aaagaacaac aagccggcag ctttctggaa
      781 acgccggcca tgcaggatgt aatggttgcg gtggccgccg gggacaagtc tctggttggc
      841 cttcaattcg gcccttatag catcgtctcg ccgctgggcg cgggcggcat gggcgaggtt
      901 tgtcgagcac acgacagcag acttggccgg gatgtcgcca tcaagacact gcctcgcgcg
      961 ttcgcaagcg accctgagag gctggctcgc ttccgtcgtg aggcccggac actcgcgtca
     1021 ctgaatcatc cgaatatcgc ggccatctac ggcctcgaag aatccggcga tatgcattgc
     1081 ctggtcttgg aactggtgga gggcgagacg ccgcgcgggc cactgacgat ggcggcggcg
     1141 cttgattgtg cgcgccagat cgccgaagcg ttggaagcgg cgcatgcgaa gggaatcatt
     1201 catcgcgacc tgaagcccgc caacgtcaag ctcacacccg aagggagagt aaaggtgctg
     1261 gatttcggtc tggcaaaagc aatctggggc gaggatatgg acggggatct ctcgcagcgc
     1321 gcgaccgttg ccgaagcaga tacgctagcc gggagtattg tcggtacgcc gtcgtatatg
     1381 agcccggaac aggcgcgcgg aaccgcagtc gatcggcgaa cggatgtgtg ggcgttcgga
     1441 tgccttttgt acgagctcct cgcaggcaag cgagcctttc agagcgaaac cgtatcggac
     1501 acgattgcgg ctgtggttgg aagcgaaccg gattggcacg ctctgccggc gaagattccc
     1561 ggcagggtcc gcgatctgtt gcggaagtgc cttcaaaagg atatcacccg caggctgcct
     1621 gacatcacgg acgcccgcag aacaatcgag aaggcgcagc gtggatggaa ccgctggcaa
     1681 attgtcgcgg ccgtgactgc tgggctggcg ccgcttgccg ttggtgcaac tctctggctg
     1741 cgcggcccgg ctcctccgcc gaatcgttcg gactggatcc agctcacgaa gtttcccgat
     1801 cccgtgagcc agcctgcgtt ttctccagac ggccggacaa ttacgttttt acggagtccc
     1861 aacaccttct ttgcggttgg ccaggtttac gttaagagtc tgccggatgg ggagccccag
     1921 caactgacga acgatggcat cgccaagacc aatcctgcgt tctctccgga cggaaccagt
     1981 attgcctata caaccttgga tgtcggattt aactgggaca cctgggtcgt tgccgcgaag
     2041 ggcggagcgc cgcggcgctg gcggcgcaac gtttccgatc tcatctggac gggcccgcgc
     2101 gagatcttat attcggaacg cccgagcaag ggaatcgtcg ccgcggaaga agatcttagc
     2161 ggaaggcaca acgtctacct tccgccgaac gatcggggcg tctcgcgccg tccccatgca
     2221 tcaccggacg ggaaatgggt cctgctcgcc gagataagcg cctatgggga ctgggagccg
     2281 tgtcgtgtgg tcccgatgga tggcagttcg accgggcggc aagtgggccc acccagcgcg
     2341 gcctgcactg gcggagcctg gtcgccggac ggcgagtggg tctatgtcac gtcgaaagcc
     2401 ggaggcttgt atcatatctg gcgccaacgc tttcccaat
//
LOCUS       DATAQL010000519         5903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4325583, whole genome shotgun sequence.
ACCESSION   DATAQL010000519 DATAQL010000000
VERSION     DATAQL010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4325583"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VHS97_08915"
     CDS             complement(<1..1895)
                     /locus_tag="VHS97_08915"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX3448361.1"
                     /translation="MSTLFEAQQSRIIELRGVRTHNLKGFDLELPLGRLICVTGVSGA
                     GKSSLAFDTLFAEGQRRYVETFSPYTRQFLSKLDKPDADNIGSIPPAIALGQPHGRYS
                     GRGTVASIAEIHDALALLYARAGQVMCRNCGQKVEPASPTTVSRAIEAWPDGTRYEIA
                     FPLDLRAQTDRAALVRSLKVEGFTRLRVNGQPEELDRPALELPEDGAVEVIVDRLVRG
                     KDPVERRTDSIETAFSRGLGRCRVIAGEKSRTFFRGWRCSRCGTDHIEPQPNLFRFNS
                     PLGLCPVCEGFGRTMELDLSRIVPDASRTIRSGALAPWSTPAYRGFLQGLLDLAAQLE
                     IPVDSPFENLTSDQLKRLLEGVSGSGFTGLKAFFRGLEGRAYKRNVRVFLSRWRRYQT
                     CPACHGARLRPEALAVKIDGRDIASLSALTIRVARAFMIGLVDLRRQPVAAGILAQID
                     SRLGYLGDIGLDYLTLDRSASSLSAGELQRVSLTKTLGSGLVNTLYVLDEPSLGIHPR
                     EVEALIRVLRRLRDQGNTLVVVEHDHDVIRSSDHIVDLGPGAGAAGGQVLYSGPLGGF
                     SQVSGSPTSDYLSRRKQAAVSGPRRPVKDRSIRLVGARGNNLKSIDVTCPLEVLSVVT
                     GVSGAGK"
     gene            complement(2041..3858)
                     /locus_tag="VHS97_08920"
     CDS             complement(2041..3858)
                     /locus_tag="VHS97_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448362.1"
                     /translation="MHTAQRAIRRLTLIGWLGLFGLTGLSAHVAEAAAPPERILPDNT
                     ILFLKLNDVKAFGEAFRGSQYGQLWNDPGLKDFREDLAQRLDDASKSLKERIGVSIKE
                     LLELPRGALALGVIGRDGADLPAAGVLIADGGENEKKLQEVLDRASKQAEEAGAKVST
                     ESFNGLSLHVVQFPKKEAQKDEKAPPRPPLVWTKSDSVFYIGSEVEIVKDVAAHRDGR
                     ENSLASNEAFAKTQAKTDANHAQAIWFLDIAKVVKAVIKATSKEGDAQAQQTDVLVSE
                     LGVYGLKSVGGSLSLASGTYDSLTKTFFNAPKPVAGLLKVFSLPPVTLRPESWVPATV
                     ATYQTLSIDLDNVYTALNELVNKFQPGMVNVLEQQLVGPNGGQPLSFQNDVFGPIGDR
                     ITVISDFKKPIKEDSQRMLAAIALEDTKAFQSTMQRLFEITGAAPEKREFQGTTIYDF
                     TVTLPNGPQGNAPQGLNGPISIAVAKDTLFVTTDTTLLEQVLRPGNSPLAESSAYQTV
                     AKEFPEKVSGLSYVRPDESARLSYDLVKSGQFEKAIQQGLAARPQGREVPNIGKLIPV
                     EKLPDFSVFAKYLSLGGGSSIMDDDGFTMTGFSLRRANP"
     gene            complement(4042..5133)
                     /locus_tag="VHS97_08925"
     CDS             complement(4042..5133)
                     /locus_tag="VHS97_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3448363.1"
                     /translation="MGEGGIVLLARCLRKFAIGLSFLGWAPFSALLAADFEVVILKDG
                     QRVTGEVVADKPNALYVDLGYDILRIPRDQILKRAKIDESSGGSKGPSRVTELDTSGF
                     YTAGLLKPSSVKELVGKFGEAVISIETPSGKGSGFLINKEGYAITNAHVIQGETRVSA
                     IMYVNVPGGFTRRRIDDVEIVSLNPFFDLALLKLPLPTDLKPSHVVLGNDDEVNAGDG
                     VFAVGNPLGLERSVTQGIVSNRSRSLEGQLFLQTDTAINPGNSGGPLFNQRGEVIGVT
                     SRGVAASFADNLGFAIPVYYVKQFINHREAFAFDKANPNSGYRYLDPPRRQNAASPEG
                     LKDRPSGAKAPVKPSEESSRPSSQKEATR"
     gene            5422..>5903
                     /locus_tag="VHS97_08930"
     CDS             5422..>5903
                     /locus_tag="VHS97_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448364.1"
                     /translation="MSIVPSWAKIHRAPIVKPWMLAAALAFIAAAPSWCQAQDAKNEP
                     PPPNQDAQPQPDPKAQVPEALDRPAEIPEVKLVPDLHTIPVPHDRPAYNLQMVNAALL
                     PRDKEGIWVLDFAFKPLRIKTVEIPGKGRRQIHYLYYKVVNRTGKPRMLVPQFIMVNE
                     "
BASE COUNT         1186 a         1804 c         1734 g         1179 t
ORIGIN      
        1 ctcttgccgg ctccgctgac gccggtcacg acgctgagaa cttcaagcgg acacgtcacg
       61 tcgatcgact tgaggttgtt gccacgagca cccaccagcc ggattgagcg atcctttaca
      121 ggccggcgcg ggcccgagac ggcggcttgt ttgcgccggc ttaggtaatc acttgtcggg
      181 gatccagaaa cctgggaaaa gccgccgagc gggccgctgt agagaacctg gccaccggcc
      241 gcgcctgcgc ctgggccgag gtcgacgata tgatccgagc tgcggatcac gtcatgatcg
      301 tgctcaacaa ccaccagggt gtttccctgg tcgcgaagac gacgtaagac ccgaatcaac
      361 gcctcaactt cacgagggtg aatgccaagg ctgggctcgt ccagcacata aagtgtgttg
      421 accaggccgg atcccagggt tttggtcaag ctcacgcgtt gcaactcccc cgcggaaaga
      481 ctgctcgctg agcggtcgag cgtgaggtaa tcgaggccga tatcgccaag gtagccaagt
      541 cggctatcga tctgagccag gattccggcc gcgaccggct ggcgccgcaa atcgaccaga
      601 cctatcatga atgcgcgggc aacgcggatc gtaagcgccg acagagaggc gatgtcgcgg
      661 ccatcgatct tcactgccaa ggcctcgggc ctcagccgcg caccatgaca cgccggacac
      721 gtttggtaac gccgccatcg gctcaagaat acgcgcacat tccgcttgta cgcccggccc
      781 tcgagtcctc gaaagaatgc tttcagcccc gtgaatccgc tccccgagac tccctcgagc
      841 agccgcttca actggtcgct cgtcaggttt tcaaaaggcg agtccacggg aatctcgagc
      901 tgagcggcca gatccagtag cccctgcagg aagcctcggt atgctggcgt ggaccaaggg
      961 gccagtgctc ccgagcgaat cgtccgggac gcgtctggaa cgatccggct caagtcgagc
     1021 tccatcgtcc gcccgaatcc ttcgcagacc ggacaaagcc cgagggggct gttgaagcgg
     1081 aacaggttgg gctgcggctc gatgtgatcg gttccgcagc gcgagcaccg ccagccgcga
     1141 aaaaaagtcc gagacttttc cccggcaatg acccggcatc ggccgaggcc cctggaaaac
     1201 gctgtctcga tcgagtcggt ccggcgttcg acggggtcct tgccacggac caggcggtcg
     1261 acgatgacct cgaccgctcc atcctcgggt agttcgagcg ccggccgatc aagttcctcc
     1321 ggctgcccgt tgacgcggag gcgcgtgaag ccttccacct tcagcgaccg cacgagagcg
     1381 gccctgtcgg tttgggcccg caggtccagt ggaaaagcga tttcgtaccg cgtgccatcg
     1441 ggccaggcct cgatggcccg agataccgtc gtcggtgagg cgggctcgac cttctggccg
     1501 cagttacggc acatcacctg gccagcgcgg gcgtaaagta acgcgagcgc atcgtggatc
     1561 tcggcgatcg aggcgacggt gccgcggcct gagtaacggc cgtgaggctg gccaagcgcg
     1621 atcgccggcg ggatactccc tatgttgtcc gcgtcaggct tatccagctt tgacaggaat
     1681 tgccttgtgt acggtgaaaa ggtctctacg taccgccgct gcccctcggc gaacagcgtg
     1741 tcgaacgcca gtgagctttt gcccgcgccg ctcacgccgg taacacagat cagacggccg
     1801 agaggaagct cgaggtcgaa acccttgaga ttgtgagtgc gaacgccgcg gagctcgatg
     1861 atccgggatt gctgagcctc gaaaagggtc gacacgggcg tggtccccct cgcggccagt
     1921 cgagcaaagc gccaaagata aggcgttgcc ctctccccac atgtacatgt atgtggggag
     1981 agggtggagg tgaggagcct tgtggagcca tcggagcaag ttgcggtccg gcagccgcaa
     2041 tcaaggattg gcgcgacgaa ggctgaatcc cgtcatggta aagccgtcgt catccatgat
     2101 gctcgagccg ccgccgagcg aaaggtactt ggcgaacacc gaaaagtcgg ggagcttttc
     2161 taccgggatc aacttgccga tgttggggac ctctcgacct tggggcctcg cggccaaacc
     2221 ctgctggatg gctttctcga actggccgct cttgaccaga tcgtacgaca gacgggccga
     2281 ctcgtcgggc cggacgtagc tcaagccgct cactttttcc gggaactcct tggcgactgt
     2341 ctgatacgcg gagctctcgg cgagtgggga gttcccgggg cggagcactt gttccaagag
     2401 cgtcgtgtcg gtggtcacaa atagagtgtc cttggcgacc gcgatgctga tcgggccgtt
     2461 gagcccctgg ggtgcattgc cctggggccc gttgggcaaa gtcacagtga aatcgtagat
     2521 tgtcgtgcct tgaaactcgc gcttttcagg tgcggcgccg gtgatctcga agagcctctg
     2581 catcgtgctt tgaaaagcct tcgtgtcctc gagcgcaatc gcggccagca tgcgctggct
     2641 gtcttccttg attggtttct tgaagtcgct gatcaccgtg atccggtcgc caatcggccc
     2701 gaagacgtcg ttctggaagc tcagtggctg accgccgttg ggaccaacca gttgctgctc
     2761 gagcacgttg accattcccg gttggaactt gttgaccagc tcgttcaagg cagtatagac
     2821 attgtcgaga tcgatactga gggtctgata ggttgcaacc gtcgcgggaa cccaagactc
     2881 gggccgcaac gtcaccggcg gcagcgaaaa cactttgagc aggccggcaa ccggtttggg
     2941 agcgttgaaa aacgtcttgg tgagactgtc gtaagtcccc gatgccaggc tgagcgagcc
     3001 accgaccgat ttgaggccgt agactcccaa ctcagatacc aggacatcgg tttgctgggc
     3061 ctgcgcgtcc ccttccttcg aggtggcctt gatcacggcc ttgacgacct tggcgatatc
     3121 gaggaaccag atcgcctgcg catggttcgc atcggtcttg gcttgagttt tcgcgaaggc
     3181 ttcgttactg gccaacgaat tctcgcgtcc gtcgcggtgc gcggcgacgt ccttgacaat
     3241 ctcgacctcg ctgccgatat aaaagacgct gtcggatttg gtccaaacca aggggggacg
     3301 aggcggcgcc ttttcgtcct tttgcgcttc tttcttggga aactggacca cgtgcaggct
     3361 cagaccgttg aacgattcgg tggatacttt tgcgccggcc tcctcagcct gcttgctggc
     3421 gcggtccagc acctcttgga gctttttctc gttctcgcca ccgtcggcaa tgagcacgcc
     3481 ggcggccggt aggtcggcac catcgcgacc gatcacgccc agggccaggg ctccccgcgg
     3541 gagttcgagc agctctttga tgctgacgcc gattcgctct ttgagcgact tggacgcatc
     3601 gtccaaccgc tgcgccagat cttccctgaa gtccttgaga cccggatcat tccagagctg
     3661 gccgtattga ctgcctcgga aggcctcgcc aaacgctttg acgtcattca gtttgagaaa
     3721 aaggatcgtg ttgtcgggga ggattcgttc agggggtgcc gcggcctctg ccacatgtgc
     3781 ggacaaaccc gtcagaccaa aaagtcccag ccacccgatg agggtcaagc gacgaatcgc
     3841 ccgctgcgcc gtatgcatgt gagaaaactc cattttcagg cacccccttg gctcaccggg
     3901 gggagatgat cgatctggcc gccggtgaaa gtaccggagt ccgtaacctc gcttcaggca
     3961 cctagccttt agcgccgtcg gttgaggccg acgagccttc cctttaccgt acaacaatca
     4021 ctgacacgag aaatccgttc tctagcgggt cgcctctttt tggctactgg gacgacttga
     4081 ttcttccgac ggcttgaccg gcgccttggc gccgctcggt cgatccttca agccctcggg
     4141 cgaggccgca ttttgcctgc gaggggggtc gagatagcga taaccactgt ttgggttggc
     4201 cttgtcaaat gcaaacgctt cgcggtgatt gatgaattgc ttgacgtagt agaccggaat
     4261 tgcgaacccc aggttgtcgg cgaacgaagc ggccacccct cggctggtta ccccgatcac
     4321 ttcaccgcgc tggttgaaaa ggggaccgcc agagttgccc gggttgatgg ccgtgtcagt
     4381 ctggagaaat agctgaccct ccaggctgcg actgcgatta ctcacgatcc cctgggtaac
     4441 ggaccgctcg aggccaagcg gattgcctac cgcgaagacc ccatcgccgg cattgacctc
     4501 gtcatcatta cccaagacaa cgtggctcgg tttcaaatcc gtaggtagtg gtagcttgag
     4561 aagagccagg tcgaaaaatg ggttgaggga cacaatctcg acatcatcga ttcgccggcg
     4621 agtaaaccca cccggcacgt tcacgtacat gatcgccgat acgcgcgtct cgccctggat
     4681 cacatgagca ttagtgatcg catagccttc tttattaatc aggaaccccg agcccttacc
     4741 cgatggcgtc tcgatggaaa tcacggcctc gccaaacttg cccacgagtt ccttgaccga
     4801 cgaaggcttg agcagccccg ccgtataaaa tcctgatgtg tcaagctcgg ttacgcgaga
     4861 cggacccttc gagccgccgc tcgattcgtc gatcttggct cgcttcaaga tttggtcgcg
     4921 gggaatgcgc agaatgtcgt agcccaaatc gacgtaaagc gcgttgggct tgtccgccac
     4981 gacctcaccg gttacccgtt ggccgtcctt gagtatgaca acttcaaaat cggctgccaa
     5041 aagcgccgaa aacggcgccc agccgagaaa gctcaggcct atggcaaatt tccgcaagca
     5101 gcgagccagc aacacgatcc ctccctcgcc caaggttcat tccgcatctt cgttggaata
     5161 aactccattg taataatcat ttcgatcctt tcaaccctgg ctgcccgcgt ttacggccgg
     5221 gcgaccatta tcagaatgat ttgtttcttt tcgctcgcct tgctacattt cggaagtggc
     5281 tgaaaatcgg cttgacggaa cttcgacgtt cgggctactt agtaaaacaa cagttcccgg
     5341 cattcatttc cagccgcgac agaacagaat cgacctatcg ccagcgcact ctaactcacc
     5401 cgagcatggg gagacgatcc cgtgtccata gttccttcat gggcgaaaat ccatcgcgcc
     5461 ccaattgtca agccatggat gctggcggcg gctctggcgt tcatcgccgc tgcaccgtcc
     5521 tggtgtcagg ctcaggacgc aaaaaacgaa ccaccgccgc caaaccaaga tgcccagcca
     5581 caacctgacc caaaagccca ggtacccgaa gcgctagacc gtcccgccga gatacccgaa
     5641 gtcaagctcg ttcccgactt gcacacgatc ccggtacctc acgatcgccc cgcctataat
     5701 ctgcagatgg tgaacgccgc gctcttgccg cgcgataaag aggggatctg ggtcctcgat
     5761 ttcgcattca agccgctgcg aatcaagacc gtcgagatcc ctggcaaggg gcgtcgacag
     5821 atccactatc tttactacaa ggtcgtcaac cgcaccggca agccgcggat gttggtcccg
     5881 cagttcatca tggtcaacga gga
//
LOCUS       DATAQL010000520         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_204224, whole genome shotgun sequence.
ACCESSION   DATAQL010000520 DATAQL010000000
VERSION     DATAQL010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204224"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VHS97_08935"
     CDS             complement(<1..890)
                     /locus_tag="VHS97_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448365.1"
                     /translation="MNRRIVFWSGLPALGLGLLLLMSCDYSAPETFNSPSGAASKLTP
                     PPPLPGAGGAADKSRGAPRGQEERTAILESSITLIQRAALQPGGKNFQYAVQKLNQYF
                     EGTPLSAYQLETPAREYLARRLPPNILKDVENRNWTLRDTRHLEDCMMYYSIATRVAK
                     TGEDLDRVRRIFDWTVRQVQLVPPGALASGRLPHVYARPYDVLLRGMATEADGLWAER
                     AWLFMVLCRQLDIDTGLITYFRSNTLNSVVPKYGVDVEKGPRSPIVWICVALIDNKAY
                     LFDARLGLEIPGPDGKGVATL"
     gene            complement(1152..1991)
                     /gene="kdsA"
                     /locus_tag="VHS97_08940"
     CDS             complement(1152..1991)
                     /gene="kdsA"
                     /locus_tag="VHS97_08940"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX3448366.1"
                     /translation="MSPPNPVSIGSVRIGRNRPLALIAGPCVMEPGDMTLLIARRLVE
                     ICGALEVPLIFKASFDKANRTSGSSFRGPGLVDGMKVFARVKEETGLPVTTDIHETIQ
                     ADPLAEVVDLLQVPAFLARQTDLLEAAAGTGRPVNVKKGQFMAPWDMTNVVAKLTDSG
                     ASGVLLTERGTTFGYGRLVNDFRAIPQMQASGAPVVFDATHSVQLPSAGAGGTASSGQ
                     REMIPTLARAAVAAGCDALFIEVHPDPDRALSDGPNSLRIDDLHGLLNTCLRIREAIT
                     AER"
     gene            complement(2127..>3058)
                     /locus_tag="VHS97_08945"
     CDS             complement(2127..>3058)
                     /locus_tag="VHS97_08945"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX3448367.1"
                     /translation="GLRSVQRLVRALRRAGFVEQLAFTPLERSALVGQSAGDPRCRCL
                     VAVGGDGTVSALLNEQPSVPLTVLPAGTENLVAQHFGLGRGPEALAATIASERPVRVD
                     VGLVGDRRFLLMVGFGFDGDIVSRHHQGRVSRCGSIRPTHRIAYVWPVLRSSFSYRFP
                     PITVRIADPDAEEVLTGTTAFVFNAPRYALGLPFVPSARDDDGWLDVIVFRKPGALQA
                     LCYLWKVFWGTHLGDSSVYHRRARHVVVSSQESIPVQIDGDPGGYLSPQTEADSDAGW
                     SINVIPAAVDIIAPANSRAQRARVTLASDGVVR"
BASE COUNT          651 a          951 c          910 g          546 t
ORIGIN      
        1 agcgtcgcca cacccttgcc gtcggggccc ggaatctcca gtccgagccg ggcgtcgaac
       61 aagtacgcct tgttgtcaat caaggcgacg caaatccaga caataggcga tcgcggtccc
      121 ttttcaacgt caacgccgta cttcggcacg acagagttga gcgtgttgct cctgaaataa
      181 gtgatcaaac ctgtgtcaat atcaagttgg cggcagagca ccatgaacaa ccaagcccgt
      241 tcggcccaga gtccgtcggc ctcggtggcc atgccgcgca gcaaaacgtc gtaaggccgg
      301 gcataaacat gtggcagtcg cccgcttgca agcgcgcctg gaggcaccaa ttgaacctgc
      361 cggactgtcc aatcgaagat tcggcgaacc cggtccaggt cttctcctgt cttggccacc
      421 ctggtcgcga tcgagtaata catcatgcaa tcttccaggt gacgagtgtc gcgcagagtc
      481 cagttccggt tctcgacgtc cttgagaatg ttcggtggca accgcctggc caggtactcg
      541 cgcgctggcg tctcgagttg ataggcgctc agtggcgtcc cctcgaaata ttgatttagc
      601 ttctgaaccg catactggaa attcttgccg ccgggctgaa gtgctgcgcg ctggatgagc
      661 gtgatcgagc tctcgagaat cgcggtccgc tcctcttgcc cgcgcggagc gccacgcgac
      721 ttgtcggcgg cgccacccgc tcccggaaga ggcggtggag gcgtgagctt gctcgccgct
      781 cccgacggcg agttgaacgt ttcaggagcc gagtaatcac agctcatcaa gagcaacagg
      841 cccaaaccca aagccggcag cccactccag aaaactatgc gacgattcaa gctgtgagac
      901 tcctagagat tccccgccgt ttgccacgtc cagggcattg ctactctcgt tgtcttttta
      961 ttgtgaaaca tgattgcgcg cgcggcgagg ccgattcctg gctcccgcca tttttccttg
     1021 ttacgaccgc gcacggacca tggttcgaca gtggcgagca accgaggaaa taatgcgttg
     1081 caacgactgg cgacaacgcc cgctcacgcg aaaagtcaat ttaagtgccg gctaggccgg
     1141 gcgatcgaca gtcaacgttc ggccgtgatg gcctcgcgta ttcgcaaaca ggtgttcaac
     1201 aagccgtgca agtcgtcgat tcgcagggaa ttaggtccat cggacagtgc ccggtccgga
     1261 tcggggtgca cctcgataaa gagggcgtcg cagccggccg cgacggccgc gcgggccaac
     1321 gtgggaatca tttcccgttg gcccgaagag gctgttcctc cggctccggc gctgggaagc
     1381 tgaaccgaat gggtcgcgtc gaagacgact ggcgcgcccg aagcctgcat ctgggggatc
     1441 gcccgaaagt cgttgaccag ccggccatag ccaaaggtcg ttccacgctc ggtcaatagt
     1501 acgcccgacg ccccagaatc cgtcagcttc gcaaccacgt tggtcatgtc ccacggggcc
     1561 atgaactgcc ctttcttcac gttgaccgga cgccccgtac cggccgccgc ctccaaaagg
     1621 tcggtttgcc gtgccaagaa ggcaggcact tggagcaaat cgaccacctc ggcgagcgga
     1681 tcggcctgaa tcgtctcgtg aatatcggtc gtcacgggca agccggtctc ttccttgacc
     1741 cgggcgaaaa ctttcatgcc gtcgaccaag cccggcccgc gaaaactcga tcccgacgtc
     1801 cgattggcct tgtcgaatga ggccttgaag atcaagggca cctcgagtgc gccgcagatc
     1861 tcgaccaaac gacgggcgat caagagcgtc atgtcgcccg gctccatcac gcacgggcca
     1921 gcaatcaatg ccagggggcg attacggcca atccggaccg aaccgatcga gaccgggttt
     1981 ggtggggaca tcctgcctcc ttgcacgtcg tgccaaactt tcgattacaa gattcgattt
     2041 gccgattcca gaatcgagca cagattgcga tcagtcaagt gcgcgcaatt cgaccggccg
     2101 atcctatcgg ccgaagccgc ttgatcttat cggacgacgc catcgctggc gagcgtaact
     2161 cgggcacgct gcgcacgact attggccggg gcaatgatgt cgacggcagc cggaatgacg
     2221 ttaatactcc agccggcatc cgaatcggcc tcggtctggg gcgataaata gccaccgggg
     2281 tcgccgtcga tctggacggg tatgctttcc tgggaggata ccaccacgtg tctggctcga
     2341 cgatgataca cgctcgaatc gccaagatgg gtgccccaaa agaccttcca gagataacac
     2401 agagcctgca atgctccggg cttgcgaaaa acgatcacgt cgagccagcc atcgtcgtca
     2461 cgagcggacg ggacaaaagg gagtcctagg gcatagcgtg gagcattgaa tacaaaggca
     2521 gtggtaccgg ttaaaacctc ctcggcgtcg ggatcggcaa tccgtaccgt gatcggcggg
     2581 aaccggtatg agaagctgga gcgcaagacc ggccaaacgt acgcgatgcg gtgggttggc
     2641 cggatcgagc cgcaacgaga gacccgccct tgatggtggc ggctgacgat atcgccgtca
     2701 aagccaaaac cgaccatcag caagaaccgg cgatccccga caaggccgac gtcgacacgc
     2761 accggtcgtt cgctcgcaat cgtggcggct aacgcttccg ggccacgtcc caggccgaaa
     2821 tgctgcgcca ccaggttttc tgtgcctgcc ggcaggaccg tcaagggcac gctcggctgt
     2881 tcgttgagca gggcggacac ggtaccatca ccgccgacgg caaccaagca gcgacaccgc
     2941 gggtcgcccg cagactggcc gacgagcgca gatcgttcaa ggggtgtgaa cgcgagctgc
     3001 tcgacgaagc cagcccgacg caacgcgcgc actaaccgct gcaccgatcg taagccgc
//
LOCUS       DATAQL010000521         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5396140, whole genome shotgun sequence.
ACCESSION   DATAQL010000521 DATAQL010000000
VERSION     DATAQL010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5396140"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VHS97_08950"
     CDS             <1..1592
                     /locus_tag="VHS97_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3448368.1"
                     /translation="FLATQLFGKWDVVLRHDLSQGLRVAAGPDADRLRRMVALLSERI
                     GEPKSWPRDPDLILALLDRLIQGILMEEDPAKRISVGVILDHAQYLVPTAELSQMASN
                     QGTRLVRLLSWAQNPYIKRHNIAFCLLCDQLAEINERLIGSAHVATLEVAMPGAAARE
                     EFATWYDSHDGKLGNLTDFTPNQLADLTAGLNLVNLERLLARAAQSGQKLDALALKRL
                     KKGLIERQARGLVEFVEPPYTLDDFVGNDAVKHRLADDAALLAKGRLDAAPMGYLICG
                     PVGTGKTYLAECFAGSVGIACVKLRNFRSKYVGETEGNLEQILNVLRAMGPVVVVIDE
                     ADAALGNREGGGDSGTSSRVFSMIASQMGDTRYRGKLIWMLLTSRPDLLPIDLKRQGR
                     AEVHLPLFSPSNDGEIEFMIKVMARKNKVSLEAGAVPAGLAGRELSGADIESIVLSAK
                     RLALTQGRDGLTRQDLEGALNDFIPSAQGLEKEKQELAAVLECTSMSFLPEQWRMRVA
                     KPDGRAKLQERMAEIRRMIEQ"
BASE COUNT          352 a          512 c          575 g          322 t
ORIGIN      
        1 actttttggc cacccagctc ttcggcaaat gggacgtcgt attgcggcac gacctgagcc
       61 aagggctgcg cgtagccgcg gggcccgacg ccgatcggtt gcggcgcatg gttgccctct
      121 tgagcgagcg gatcggcgag ccaaaatcct ggccccggga tcccgacctg atcctcgcgc
      181 ttttggaccg cttgatacag ggtattttaa tggaagaaga tcctgccaag cgaatcagcg
      241 tgggtgtgat cctcgaccat gcacagtatc ttgtacccac ggcagagctc tcccagatgg
      301 cgtccaacca gggaacgcgg ctggtgcggc tgctctcatg ggcccaaaat ccgtatatca
      361 agcgtcataa tattgctttt tgcctcttgt gcgatcagct tgccgagatc aacgagcggc
      421 tgatcggcag cgctcacgtg gcgacgctcg aggtcgccat gccgggcgcc gcggcgcgcg
      481 aagagtttgc gacctggtat gacagccacg acggcaagct tggcaatctg accgacttta
      541 cccccaatca attggccgat ctgaccgcag gattgaacct ggtgaatctc gagcgtctac
      601 tagcgcgcgc ggcccagtcg ggccagaagc tcgacgcgct ggcactcaag cggctcaaga
      661 agggtttgat cgagaggcag gcgcgcgggc tggtcgagtt tgtcgagccg ccttacacgc
      721 tcgacgactt tgtgggcaac gacgcggtca aacaccgtct ggcggatgac gccgccctcc
      781 tggccaaagg gcggctcgac gctgctccga tgggttatct catctgcggt ccggtcggca
      841 ccggcaagac ctatctggcg gagtgcttcg cgggttcggt cggcattgcg tgcgtcaagt
      901 tgcgcaattt ccgctccaag tacgtcggcg agaccgaggg caacctggaa cagatcctca
      961 acgtgctgcg cgccatgggg ccggttgtgg tcgtgattga cgaagccgac gcggccttgg
     1021 gcaatcggga aggggggggt gactcgggaa cctcgagccg agttttctcg atgatcgcct
     1081 cgcagatggg agatacccgc tatcgcggta agctgatctg gatgcttttg accagccggc
     1141 cggacctgct gccgatcgac ctgaagcggc aagggcgcgc cgaggtccat ttgccgcttt
     1201 tcagcccgag caatgacggc gagatcgaat tcatgatcaa ggtgatggcc cgcaagaaca
     1261 aggtaagcct ggaggccggg gccgtgccgg cgggacttgc cggccgggaa ctctccggtg
     1321 ccgacatcga gagcatcgtg ctttcggcga aacggctcgc gctcacacag gggcgcgacg
     1381 gactgactcg ccaggatctc gaaggggctc tgaacgattt catcccgtcg gcgcaggggt
     1441 tggaaaaaga aaagcaagaa ctggctgcgg tgctcgagtg cacctcgatg tcgttcttgc
     1501 cggaacagtg gcggatgcgc gtcgccaagc cggacgggcg ggctaagctg caggagcgga
     1561 tggccgagat ccgccgcatg atcgagcagt gaccgacaaa gaccattcac ccgaggcaga
     1621 aaccatgagc gcgaaaagta ccaagggaca tggccgtacc ccgtggctcg acgagaaatc
     1681 cgaaactccg ttgatcgatg actatgtgca acgactcgga tcgttcctgg aagcgatggc
     1741 cgacggccga atcgacgtga g
//
LOCUS       DATAQL010000522         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1884484, whole genome shotgun sequence.
ACCESSION   DATAQL010000522 DATAQL010000000
VERSION     DATAQL010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884484"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1050)
                     /gene="prpB"
                     /locus_tag="VHS97_08955"
     CDS             complement(175..1050)
                     /gene="prpB"
                     /locus_tag="VHS97_08955"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEX3448369.1"
                     /translation="MITPGARLRAAWSASPIMIPGAFNALVAKLAERLGYKAVYLSGG
                     ALSAGWAGLPDIGLLTQSEFAGQAAVLARATTLPVLCDADTGFGEAINVERTVRLYEE
                     AGVAGLHLEDQVMPKRCGHLSGKSLVDARTMASKVRAAVQARRDPDFVIVARTDARGV
                     EGFDAAVNRAREYLAAGADMIFPEALESADEFARFAREVKAPLLANMTEFGKSPLLLF
                     DDLAAMGYKAVIYPLTAMRAAMHAAEEALRTVRDQGTQQGLLDRMQSRVELYDLLGYK
                     DWEERDRGYFTPDSK"
     gene            2119..3084
                     /locus_tag="VHS97_08960"
     CDS             2119..3084
                     /locus_tag="VHS97_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448370.1"
                     /translation="MAIDPQNGTWRPILTGGLSIGSGPVSPDGRYLLYSSLGPDPDPD
                     QLGIWVYDMSGATAHRRNLKRKGDSFWTSHGRQVVIAVLNGPPSKFETWRVNFDGTGA
                     VKLPRSDTELVLDCSADGTWLVTRRMTGPGDAARPDRLKLIHLDGAGARDLTRGAPPD
                     ALIHAPKISQDGRSLAYAEIHTVNGPQQSRLVVEEISGQHRREVPTPAALGRVGGICW
                     SPDGSRLALNTIDIRNKTGEIAVVSLDGSSFRNLPCRPAAGISSSAIGLGSQRVSAPH
                     RRLNWWTRKHPEAAMRRWFSNLKRRREPSARSSTKPRPTQSGLVS"
     gene            3081..>3386
                     /locus_tag="VHS97_08965"
     CDS             3081..>3386
                     /locus_tag="VHS97_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448371.1"
                     /translation="MSENACRDRAYLARFLEIVESAPDDPAAVDALIWIVTFGFHGRH
                     FDRAIDILVERHVGRRKVGESAANLCRSASPSAEKLLHAVVANGRPASTDDLRNGNLT
                     "
BASE COUNT          606 a         1104 c          994 g          682 t
ORIGIN      
        1 aggtacgcca tcccgagatg gcctggctca ctcgaaccag catccttgtc ggcatcgacg
       61 acgcggatga cggtgtgcct gccgaattca agatctgttc tcttctgcac gtcactgccg
      121 tagaaccgtt tggccccacc cctgccagcc cgagtcaggt cgaggccgga taaatcactt
      181 ggaatcaggc gtgaaatacc ctcgatcgcg ttcttcccaa tctttgtagc cgagcaagtc
      241 atacagctcg actcggctct gcatgcggtc gagtagacct tgttgcgtac cctggtcgcg
      301 caccgttcta agggcttctt cggccgcgtg cattgccgcg cgcatggcgg tcagcggata
      361 gatcaccgcc ttgtaaccca tcgccgccag gtcgtcgaac agcaaaaggg ggctcttgcc
      421 aaactcggtc atattggcca acaagggtgc cttgacttct cgcgcgaagc gagcgaattc
      481 gtcggccgac tcgagggctt ccggaaagat catgtcggca ccggcggcca ggtattcgcg
      541 cgcccggttc acggcggcgt cgaagccctc aacgccccgc gcgtctgtcc gcgcaacaat
      601 cacaaaatcg ggatcgcggc gcgcctgcac ggcagcccgg accttgctcg ccattgtccg
      661 cgcatcgacg agcgactttc ccgaaaggtg gccgcaccgc ttgggcatga cctggtcttc
      721 caggtgtagc cccgcgacgc ccgcctcttc atacagtcgc acggtgcgct cgacattgat
      781 tgcttctccg aagcccgtgt cggcatcgca caagacaggc aacgtcgtcg cccgcgccaa
      841 aaccgcggcc tggccggcga attccgactg agtcaagagt ccaatgtcgg gcagaccggc
      901 ccagccggcc gagagagcac ctcccgaaag atagacagcc ttgtaaccga gccgctcggc
      961 cagcttggcg accagggcgt tgaatgcgcc cgggatcatg atcggactgg ccgaccacgc
     1021 cgctcgcaac cgggctccgg gtgttatcat agcggaagtc ctcctcggtt cattcgtcgc
     1081 gatttcgctg tccacttact gagcacagtt tacaggctat tcctaactgc atactccgta
     1141 atgcctaccg cctacttttc acccatttgg tttcgttaaa gtccggaagg cacacacgcc
     1201 ttgaccgtca acttcgcggg cgagtttgca aagttacgtc aaaaagcgcg tggcactttt
     1261 tggcacaaat tggcacccct gggaaagttt tcagtgccaa ctcgccctgg tcgacgcaga
     1321 tatcgctcgg gcgcaagtcg ccaattttct gaccgttttg tcgatggaat ccgcttcggt
     1381 ccggcaccgt ccggccgatg aatttgggcg aatatgcgca ctcctccgcc cgtgacgtaa
     1441 gtccgcagcg cctgggagcg acgaagtgga tcccgggcat cgttcaattt tagcatcttc
     1501 gcaggtgaca agagctgggt ggctggggtc gagctggcga cagccagcga gcccccggcg
     1561 cggaagcctc ggatctgggg gcgtcaccct tcgggcgtcg atcacagcca ccccctgcgg
     1621 tgcaaccagc ttttgaacct tgctggatcc cgatcgcaca ttctggatcc aaattcggtg
     1681 tctttccgcc cagtctttgc gctgtcggac tttccggccc ggcggccgcg gcggtaagcg
     1741 caggttcgtt cttcgctttt tcacaggcgt cgatggcatc gttcgtcacg ttttcgcagc
     1801 agtcgggttc gtcacgtttt cgctgttggc gtggcttcgt tcgtcgcttt ttcgcaggcg
     1861 tcgttagaga ttcgcccgac gttgatcagt tgtcggccta tctcacactc acggatgcag
     1921 ccgtccttag ccgtcgattc tccggtcatg gggacaaacg ccattcggta acagcttttc
     1981 atcgaggctc ctctcggccc cgctcgcagt tctcagcccg gcgcccaggc tggccgacat
     2041 gccctcgtgg ggcaaggagg actagaacgg tcgcaacggg tgtagcggtt ttgcggtgag
     2101 tacggcatct cggggattgt ggcgatcgac ccgcagaatg gcacctggcg ccccatcttg
     2161 acgggcgggc tctcaatcgg ttcaggcccc gtctcccccg acggtcgcta cttgctgtac
     2221 tcgagtcttg gtcccgatcc cgatcccgat caactcggca tctgggtcta cgacatgagc
     2281 ggagcgaccg ctcaccggcg aaacttgaag cggaagggtg actcgttctg gacgagccac
     2341 ggccgccagg tcgtcatcgc cgtcctgaat ggaccaccgt cgaaattcga aacctggcgc
     2401 gtgaattttg acggcacagg cgccgtcaag ctgccgcgct ccgacaccga gctcgtgctg
     2461 gactgctcag ccgacggaac atggctagtc acacgtagga tgaccggtcc cggtgacgcc
     2521 gcccggcccg accgcctcaa gttgatccat ctcgatggcg ccggagcacg cgatctgacc
     2581 cggggagcgc ctccggatgc gctaatccac gcccccaaga tctcccaaga cggccggagc
     2641 ctcgcttacg cggagattca cacggtcaac ggaccgcagc agtccaggct cgtggtcgag
     2701 gagatctcgg gacagcatcg ccgcgaggtt ccgaccccgg ccgctttggg cagggtcggg
     2761 gggatttgct ggtcgcccga tggttcgcga ctggcgctca acaccatcga catccgcaac
     2821 aagacgggtg agatcgcggt cgtcagcctc gacggctcga gctttcgcaa tctcccctgc
     2881 cgcccggccg ctggaatatc cagctctgcg attggtctgg gctcacagcg ggtctccgca
     2941 ccgcatcggc gattgaactg gtggacccga aaacaccccg aggccgctat gaggcgctgg
     3001 ttctcgaatc tcaaaaggcg tcgcgagcct tccgcgagga gctccacaaa gccaagaccg
     3061 acgcagagcg gactcgtttc ttgagcgaaa acgcctgtcg ggaccgggcg tatctggcgc
     3121 gtttcctcga aatcgtcgaa tctgccccgg acgaccccgc ggccgtcgac gccctgatct
     3181 ggatcgtcac tttcggcttt cacgggcgcc attttgaccg cgccatcgat attctcgtcg
     3241 agcgccacgt cgggcggcgc aaggtcggtg agtccgccgc gaacttatgt cgctcggcct
     3301 cgccgtcagc cgagaagcta ctccacgccg ttgtcgccaa cgggcgtcct gcatccacgg
     3361 acgatctcag gaatggtaat ttgaca
//
LOCUS       DATAQL010000523         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1732474, whole genome shotgun sequence.
ACCESSION   DATAQL010000523 DATAQL010000000
VERSION     DATAQL010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732474"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..2152
                     /locus_tag="VHS97_08970"
     CDS             146..2152
                     /locus_tag="VHS97_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448372.1"
                     /translation="MRRMPSCAQRTVTRRAQPLLEGLEERRLLSHITAFKASQTAASP
                     SGVFSSAGNTFTYKTPSGGLATIQVVGLGNLAGTTVDQNGDLNLVFGGTNAFSKIVGN
                     VKGGNGRAPLESILNSQLIAAGQQNSLSGVGGNILKAVYLKNFDLISGGNINLTSGVN
                     TLQLDSIGSNTQIHLRQLPPAPTTTTTTATSTPVLVNTTSGTTGAVITGVSSSTSTST
                     ENTLVAFESGTFTTNGVTTTYKSNGNGAQTLTAISGSFTAGGNQSESLPAGQPSQTIP
                     PAPPGVILQVNRIAGKTSGPINLLTDPNVFAYDPTTGNVVRFAINLATGKGTQNPLAN
                     PIVVANDPATVGLNLGRNGNQLVLLVSAGSTVTAYNATTGAEVGFFTTPQDTYSIGST
                     DTLTVLGSYQSNINQQIQAINLSASLASHQLVLASPSTSYSPTAESGLILLGGLAGVS
                     GSNTIYTAVEAFFNTFVPTEYQLGQQPINTVNVQTTDHQSVASYKLTAGTASAVTLNP
                     PFTSLGAGSMPGPTQPGAALGSIDQSLAWILSPPSNGTTTISLSSGGKITLTYPDQLV
                     SLSEAYRPDLAGAAVIDIQGHVQSIRGGSATGMVLNDTGNLNLVKLVSAKNSTIIGQP
                     VSHLQIQHRSKVTVLTPSRAVGMRNGVTVDSELQPIGPISLTGD"
     gene            complement(2205..>2868)
                     /locus_tag="VHS97_08975"
     CDS             complement(2205..>2868)
                     /locus_tag="VHS97_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448373.1"
                     /translation="APAAEPIGPAEGRVSPSEDARLGWCVDAAIGELTGRRTSRSVDS
                     SAATRPPPRRSDWRDVLTNIRRDRLARPLRSAFDELLIDALGEAGLCEPLMKRLALIP
                     PSEAGSRVWQALENCAMQRLARMVEDGEKARRLGRALDLWDEVMGLSWPGNSRFPSQF
                     TERVSALAWDDSSRRRLWLDRLRRTGAAMAGKAGRHPQAEVVAAERARVQATLGGLGS
                     GH"
BASE COUNT          616 a          938 c          803 g          511 t
ORIGIN      
        1 gagtttgcgt cgatgggaga aaacccggaa cttgggttat gcctagaggt cggtttgccg
       61 aattgattag tgaggtcgat cgggctgagt tccgccggct gcggcggtcc cggaccaaaa
      121 ggagtcggtc atcgggagga acgcaatgag gcggatgcca tcgtgcgctc aaaggacagt
      181 gacgcgtcgt gctcagccgt tgctcgaggg actggaggag cgccgtctgc tcagccacat
      241 taccgctttc aaagcgagcc aaacagccgc cagcccatct ggagtatttt cgagcgccgg
      301 caacacgttt acgtataaga cgccttccgg cggcctggcg accattcaag tggtgggact
      361 cggcaatctg gccggaacca cagtcgatca aaacggcgac ctcaatctgg tgttcggcgg
      421 caccaacgcc ttctcgaaga tcgttgggaa cgtcaaaggt gggaatggcc gcgcgcccct
      481 ggaaagcatc ttgaacagtc agctcatcgc ggccggacaa caaaacagct tgagcggcgt
      541 tggcggcaac atcctaaagg cggtttacct caagaatttc gacctcattt cgggcggaaa
      601 catcaacctc acctcgggtg tgaacacact gcagctcgac tcgatcggat caaacactca
      661 gatccaccta cgacaacttc cgcccgctcc taccactacg acgaccacgg cgaccagtac
      721 tcccgtgctc gtgaacacga cgagcgggac aacgggggcc gtgatcaccg gcgtctcgtc
      781 gtccactagc acgtccactg agaatacact cgtggccttt gaatcgggga cgttcaccac
      841 caacggtgtc acaaccacgt acaagagcaa cggcaacggc gcccagacgc tcaccgccat
      901 atcgggaagc tttaccgcgg gcggcaacca gtccgaatcg ctcccggccg ggcagccctc
      961 gcagaccata ccccctgctc ccccgggagt gatcctccaa gtcaatcgaa tcgccggcaa
     1021 gacgagcgga ccgatcaacc tactcacgga cccaaatgtt tttgcgtatg atccgacgac
     1081 tggcaacgtc gtccgattcg ccatcaatct ggccaccggc aagggcacgc aaaacccgct
     1141 ggccaacccc atcgtcgtcg cgaatgatcc cgcaacagta ggattaaacc tgggccggaa
     1201 cggtaaccag cttgtactcc ttgttagtgc ggggtcgacc gttactgcct acaacgcgac
     1261 aaccggtgca gaggtgggat tctttacgac acctcaggat acctattcga tcggttcaac
     1321 agatacgctc accgtccttg gcagttacca gtccaacata aatcaacaga tccaggcaat
     1381 caatctgtcc gcgagcttgg cgtcgcacca attggtattg gccagcccgt cgacctctta
     1441 ttctccgacc gcggaatcgg gactcatctt gctgggtgga cttgcaggcg tatcgggatc
     1501 gaacacgatt tacaccgcag tggaggcctt ctttaacacg tttgtgccga ctgaatacca
     1561 actcggccag caaccgatca acaccgtaaa cgtccagacg accgaccatc aatcggtcgc
     1621 gtcgtacaag ttgaccgctg gaactgcttc agccgtcacc ctcaacccgc ctttcacatc
     1681 cttaggtgct ggcagtatgc ccggcccgac ccagcccgga gcggcccttg gcagcatcga
     1741 tcaaagtctc gcgtggatcc tgtcgccgcc ctccaatggc acgactacga tcagcctctc
     1801 gtccggcggc aaaatcacgc tgacctaccc cgatcaactg gtcagcctga gcgaggccta
     1861 ccgtccggat ctcgccggcg cggccgtgat cgatatccag ggccacgttc agtcgatacg
     1921 cggcggcagt gcgaccggca tggtcttgaa cgacaccggc aacttgaacc tggtcaaact
     1981 cgtgagcgcg aagaactcca cgatcatcgg ccagccggtc agtcacctgc agatccagca
     2041 ccgctcgaaa gttactgtac tcacgccgtc gcgcgccgtg ggcatgcgca acggcgtgac
     2101 tgtcgactca gagctgcagc cgatcggacc gatttcactg accggtgact gacgacacag
     2161 ccagtcgccg gtcggcaagt gtcggaacca agccaatttg ccgattaatg cccggaaccc
     2221 agacctccga gtgtcgcttg gacgcgcgcc cgttcagccg ccacgacctc tgcctgcgga
     2281 tgccgtccgg cttttccggc catggcggct cccgtgcgcc gtagccgatc gagccagagc
     2341 cggcggcggg aagagtcatc ccacgctaat gccgaaacgc gctcggtgaa ctgggaaggg
     2401 aaacgagagt ttccaggcca gctcaatccc atcacctcgt cccaaaggtc gagagcgcga
     2461 ccgaggcgtc gagctttttc gccgtcctcc accatccgag cgagacgctg catcgcgcag
     2521 ttttcgaggg cctgccacac gcgcgatccc gcttctgacg gcggaatcag ggccaatcgc
     2581 ttcattagcg gctcgcaaag tccggcttcc cccagggcgt cgatcaacag ttcgtcaaag
     2641 gccgaccgta gcgggcgggc aagccgatcg cggcgtatgt tcgtcaagac gtcacgccaa
     2701 tcagatcggc gcggcggcgg tcgcgtagct gcggagctgt cgaccgagcg agacgtgcga
     2761 cgtcccgtca attcgccaat cgccgcatcg acacaccagc ccagtcgggc gtcttccgaa
     2821 ggggacacgc gtccttccgc cggtccaatc ggctcggcag ctggcgcg
//
LOCUS       DATAQL010000524         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3870127, whole genome shotgun sequence.
ACCESSION   DATAQL010000524 DATAQL010000000
VERSION     DATAQL010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3870127"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2225)
                     /locus_tag="VHS97_08980"
     CDS             complement(<1..>2225)
                     /locus_tag="VHS97_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX3448374.1"
                     /translation="VNQVVPARITWTDLARDIERKKPAEVSKVIAAIRADALYETAST
                     YGIPNPPDDASLSQGVAALRRFLAAFPSHPQAVRAAFFLGESYRARGKSSDALDAYTQ
                     FLKEDGFHVDTDVARRDWAELVMTASFHVGEILQGQQKFAEAIAAWKGYLSKFPNGPQ
                     SADAQRAILDTQLLIAADHAQRERYPEARAAWTDFVIQNPLDARAPLVLFQIGESLAA
                     EKKFDQAITGWETLASKFPGSEPSAHGQFMAASFFENEKGNPSEAIERFKKIAVEPWA
                     AQARQRIAVMESKALGVTTPRTFRSGEDAHLNVITRNIETLSFAAYKLSAEAYFRKKN
                     ALSNVESLDIGLVAPDASWTVSVPGFARYKPVESHYELKKLDLPGVYVVKVTDEKTLQ
                     ATTLVIGSDVDAIVKSSRDQILVFAEDMKTGAGRPGARILVADGGQVVLEGVTGADGT
                     LLRDWKPPRAGNGHLSYLVLDGRHVAGSGLGIPEQVAQGLSPRAYIYTDRPAYRPGQK
                     VSVRGVVREVADGQYASTPGSIYRFEVADSRGRLIVARPVTLSPFGTFHESVLLDSAT
                     PVGDYQVRVFQPGKSDFHGNFQVESYQLEPIDLTFDLKTTVFYRGETIAADLIARYQY
                     GAPVAGRPIDVQLPDGRILHSLTDAAGKYHFECPTDGFAEEQALDITARLPQDNVAAV
                     ARVLLAVRGFTIELTTSRDVYLDGESFQLQVVTTDARGEPAGHKLSAALLKLVESQGN
                     IT"
BASE COUNT          410 a          682 c          705 g          428 t
ORIGIN      
        1 gtgatgttcc cttgactctc gaccagcttg agcagagcgg ccgaaagctt gtgaccagcg
       61 ggctctcctc gcgcgtcggt cgtgacaacc tggagctgga acgactcgcc gtcgagatag
      121 acgtcgcgcg acgtggtcag ctcgatcgtg aatccgcgaa ccgcgagcaa gactcttgct
      181 acggccgcca cgttgtcctg gggaaggcgg gccgtgatat cgagggcctg ttcctcggcg
      241 aagccgtcgg tgggacactc gaaatgatac ttgccggcgg cgtcggtcag ggagtgcagg
      301 atgcggccgt cggggagctg gacgtcgatc ggccggcccg caaccggcgc tccatactgg
      361 tagcgggcaa tcaagtcggc cgcgatcgtc tcaccgcggt agaagaccgt cgtcttcaag
      421 tcgaatgtca gatcaatcgg ctcaagctga tacgactcaa cctgaaaatt accatggaag
      481 tcgctcttac ctggctgaaa tacccggacc tgataatccc ccaccggcgt ggccgaatcg
      541 agcagaacgg attcgtgaaa ggtgccgaat ggcgacaagg tcaccggccg agccacgatc
      601 agcctcccac ggctgtcggc gacctcaaag cggtagatcg agccgggagt actcgcatac
      661 tggccgtcgg caacctcgcg aaccaccccc cggaccgaga ccttctgtcc tggccgatac
      721 gctggccggt cggtgtagat gtaggcgcgc ggactgagtc cttgcgcgac ctgttcggga
      781 atacccagtc ccgagcctgc gacgtggcga ccatccagga cgagatagct caggtggcca
      841 ttgcccgcgc gcgggggctt ccagtcgcgg agcaaggttc cgtcggcccc ggtcacccct
      901 tcaagcacca cctggccgcc gtcggcgacc aagatccgcg ccccgggccg gcctgcaccg
      961 gtcttcatgt cttcggcaaa caccaggatc tggtcgcgtg agctcttgac gatcgcgtct
     1021 acgtcgctgc cgatcacgag cgtcgtcgcc tgcagcgtct tttcgtctgt caccttgacg
     1081 acgtagacgc ccggcagatc gagctttttg agctcgtaat ggctctcgac cggcttgtac
     1141 cgggcgaagc caggcaccga aaccgtccac gatgcatcgg gcgcgaccag cccaatatca
     1201 agcgattcga cgttactcag cgcattcttc ttacgaaaat aggcctctgc gctcagcttg
     1261 taagcggcga agctgagcgt ctcgatattg cgcgtgatga cgttcaggtg ggcgtcctca
     1321 cccgatcgaa acgtccgtgg agtagtcacg cccagcgcct tcgattccat cacggcgatc
     1381 cgttgtcgcg cctgagccgc ccatggctcg acggcgatct tcttgaaccg ttcgatcgct
     1441 tcggatgggt tgcccttctc gttctcgaaa aatgatgccg ccatgaactg gccatgtgcg
     1501 gacggttcgc tgccggggaa cttcgaggcc agggtttccc agccggtgat ggcctgatcg
     1561 aatttcttct cggccgccag gctttcacca atttgaaaga gcaccagcgg cgcgcgggcg
     1621 tccagcgggt tctgaatgac gaaatcggtc caggcggcac gggcctcggg atagcgctcg
     1681 cgctgggcgt ggtcggcggc gatcagtagt tgagtgtcga ggatggcgcg ctgggcgtcg
     1741 gcgctctggg gaccgttggg aaatttcgac agatatccct tccaggccgc gatggcctcg
     1801 gcgaatttct gttgcccttg gaggatctca ccgacgtgaa acgaggctgt catgacgagc
     1861 tcggcccaat cgcgacgagc cacgtcggta tcgacgtgga agccatcctc cttgaggaac
     1921 tgagtatagg cgtcgagtgc atccgagctc ttgccgcggg cccgatacga ttctcctaaa
     1981 aagaaggccg cacgcacggc ctgcggatga gacgggaacg ccgccaggaa ccgccgaagc
     2041 gccgcgaccc cctggcttaa gctcgcatcg tcgggaggat tggggatgcc atacgtcgat
     2101 gcggtctcat agagtgcgtc ggcacgaatg gcggcgatga ctttggagac ttcggctggc
     2161 tttttgcgtt cgatgtcgcg ggcaagatcg gtccaggtga tgcgggccgg cacgacttgg
     2221 ttcac
//
LOCUS       DATAQL010000525         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3409910, whole genome shotgun sequence.
ACCESSION   DATAQL010000525 DATAQL010000000
VERSION     DATAQL010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3409910"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..896)
                     /locus_tag="VHS97_08985"
     CDS             complement(219..896)
                     /locus_tag="VHS97_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448375.1"
                     /translation="MDKNMTAQQIATDLPQNLDYRPTSPMAVLSVVIGLLALVSACLV
                     YDLGPTTFEIVSSIGLVVSLESLSVVRRYELRGRGAAKAGLGLSVAALVAGFGIISLQ
                     SAFEVPAGCLGISYEALQPKPGESIPASARVLDGKKVFIKGYMYPSSQMTGIREFVLC
                     RDNGTCCFGGQPKLSDMIQVNLKEPLALDYQTGLRQLAGTFRVVPNATSVGLGSVLYR
                     LDADYAR"
     gene            complement(966..2324)
                     /locus_tag="VHS97_08990"
     CDS             complement(966..2324)
                     /locus_tag="VHS97_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3448376.1"
                     /translation="MSLIAIAWRNLTQRALASALTGFSMALGVSLVVAVLVIHHVVSE
                     SFQRNSGGGYDVIVGAKGSKLQLVLNTVYHLSTPVENIPYSVLHDLTDGQYRTYVARA
                     LPYCLGDNYEGHRVVATVPELFQDSEYAAGRKYAFKEGRNINPTGYFEGVVGALAAAR
                     TGLKLGATFRPTHGVNDDGQGHDHEAFTVVGILEPTGTPNDRAIFVNLEGFYLLENHA
                     KPAGEAHRPGPASPDQHANGHDEHEKPHDHAHGGHDHGDDEGHELIPLPEDQRELTAI
                     LVRTSSPAAAVRLPNLINEGTVAQAVLPIREIRALFDVIVGPIETVLLLLTALIVVVS
                     AIGILVSIYTSMSERRREIAVMRALGARRSTVRTIVLLESILLSLNGGLAGWVLGRTL
                     IAALSPAIAARTGVVIGFAPPITAELALIPGLILLAALAGYLPAMTAYRTDVAQALAS
                     SP"
     gene            complement(2321..>3046)
                     /locus_tag="VHS97_08995"
     CDS             complement(2321..>3046)
                     /locus_tag="VHS97_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3448377.1"
                     /translation="RQRAHATLHTPMSREDPEMLLINNVKKSFQEPDGRRLPILDVDD
                     FRVDDGEQAVLLGRSGCGKTTLLHVIAGISTPDSGEVRINGLDITQLEEAGRDRFRAE
                     TVGYVFQSFNLLSGFTALENVLLGMAFAGGRRDVARARDLLGRVGLSHRHRHKPAELS
                     VGEQQRVAVARALANRPRLLLADEPTASVDARHQQTVIELIRERCEEEGVALLLVTHA
                     PEVSAQFERVVHLENLNKVAAQV"
BASE COUNT          542 a          973 c          963 g          568 t
ORIGIN      
        1 tggctccccc cggtttcacg attagcggcc acgacctcct tctccatggc caatgttcgg
       61 cttgctccgc gctccaggcg ccctgatgcc ggtcgttcgt ccattcgccc atatttcatc
      121 ggacctccac gatggtcgag atcagacctt tctgctcttc cctcccgctc ggacgacgct
      181 tctcggaaat ggtccgcgcg cgaaatcctg agagaatttc atcgcgcata atccgcgtcg
      241 agtcggtaaa gcacggaacc caggccgacc gaggtggcgt tcggcaccac acggaatgtc
      301 ccggccagtt gccgcaagcc cgtctgatag tcgagcgcga gcggctcctt gagattcacc
      361 tggatcatgt cggaaagctt gggctggccg ccgaagcaac atgtcccgtt gtcgcgacag
      421 aggacgaact cgcggattcc cgtcatctgg ctgctcgggt acatataacc cttgatgaat
      481 accttcttcc cgtcgagtac cctcgccgac gcggggatcg actccccggg cttgggttgc
      541 agcgcctcat aactgatgcc caggcagccc gcaggcactt cgaacgcgga ctggaggcta
      601 atgattccga atccggcgac cagggccgca acggacaggc ccagaccggc cttggcggca
      661 ccgcggccgc gcaattcgta acgacggaca acggagaggc tctcgagact cacaaccagg
      721 ccgatggagg agacgatctc gaatgtcgtc gggcccaggt cgtaaaccag acatgccgag
      781 accagggcaa gtagccctat gaccacgctc agcacggcca tcgggctcgt cggccggtag
      841 tcgagattct ggggaaggtc ggtcgcgatt tgttgggcag tcatattctt gtccatagct
      901 ttcgtccctc ggtcccccta attggtcctc actctctgca cgatctcgat ttcgtcaccg
      961 cgcgatcacg gcgatgatgc aagcgcctgc gcgacgtcgg tccggtaggc ggtcattgca
     1021 ggcaggtaac cggccagggc agccagcaag atcaggcccg ggatgagcgc caactctgcg
     1081 gtgatcggcg gcgcgaatcc gatcactact cctgtccggg cggcgatcgc cgggctcagg
     1141 gccgcgatca aggtccggcc gagcacccac cccgccaggc ctccgttcag cgagagcagg
     1201 atcgactcga gcagcacgat cgttcgcacc gtcgaccgcc gcgcgcccaa ggccctcata
     1261 acggcgatct cgcgacgccg ctcgctcatc gacgtgtaga tgctcaccag gatgccgatc
     1321 gcggaaacga ctacgatcaa tgctgtgagc aagagcaaga cggtctcgat cggcccgacg
     1381 atcacgtcga aaagcgcacg gatttccctg atcggtaaga cggcctgcgc caccgtcccc
     1441 tcattgatca gattcggcag gcgaaccgcc gccgcgggcg atgaggtgcg gaccaggatc
     1501 gccgtcagct cccgctggtc ctccggcagg ggtatcaatt cgtggccttc atcgtcgccg
     1561 tggtcgtggc ccccgtgtgc gtggtcgtgc ggcttctcat gctcatcgtg gccgttcgcg
     1621 tgttggtcgg gggaagccgg cccgggtcgg tgcgcttcgc cggcgggctt ggcgtggttc
     1681 tcgagcaggt agaagccttc caggttgacg aagatcgcgc ggtcgttcgg cgtcccggtg
     1741 ggttcgagaa tgccgacgac cgtgaaggcc tcgtggtcgt gcccctgtcc gtcatcgttg
     1801 acgccgtgcg tcggccggaa cgtggcgccg agcttcagcc cggtccgcgc cgcggccagg
     1861 gccccgacga ccccctcgaa gtagccggtg gggttgatat tccggccctc cttgaacgca
     1921 tatttgcggc ccgccgcgta ctccgaatcc tgaaacagct cgggcacggt ggcgaccacc
     1981 cggtgacctt cataattgtc gcccaggcag tacgggagcg ccctggcgac gtaagtcctg
     2041 tactgtccgt cggtgagatc gtgcaaaacg gagtaaggaa tgttctcgac gggcgtgctc
     2101 aggtggtaca cggtgttcag tacaagctgg agcttgcttc ccttggcccc gacgatcaca
     2161 tcgtagccgc ccccggagtt gcgttgaaat gactcggaga cgacgtggtg gatgactaag
     2221 accgccacca caagtgagac gccgagggcc atcgagaatc cggtgagagc agaggcgagc
     2281 gctcgttgag tgaggttccg ccaggcgatc gcgatgaggc tcatacttgg gccgccactt
     2341 tgttgaggtt ctccagatgg acaactcgtt caaactgggc cgagacctcc ggggcatgcg
     2401 tgaccaagag gagggcgaca ccctcttcct cgcagcgttc gcggatgagc tcgatcacgg
     2461 tttgctggtg gcgggcgtcg acgctcgcag tcggctcgtc ggcgaggagg agtcgcggcc
     2521 ggttcgccag ggcccgagcg accgccaccc gctgctgctc gcccacggag agctcggcgg
     2581 gcttgtgccg atgccggtgc gacaggccca cccggcccag taggtcccgt gcccgggcga
     2641 cgtcgcgccg acctccggcg aacgccattc caagtaagac attttccagc gcggtgaacc
     2701 cggacaagag attgaacgac tgaaagacgt agccgacggt ctcggcgcgg aacctatcgc
     2761 ggcctgcctc ctcgagctgt gtgatatcga gaccgttgat gcggacctcg cccgagtcgg
     2821 gcgtgctgat tcctgcaatg acgtgcaaga gcgtagtctt gccgcagcca ctccgcccaa
     2881 gaagcacggc ttgctccccg tcatcgacgc ggaagtcatc gacgtccaga atcggcagcc
     2941 ggcgtccgtc gggttcttgg aaggacttct tgacgttatt aatgagtagc atttctggat
     3001 cttcgcggct catgggagtg tgcaaagtgg catgggcccg ctgtct
//
LOCUS       DATAQL010000526         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2648455, whole genome shotgun sequence.
ACCESSION   DATAQL010000526 DATAQL010000000
VERSION     DATAQL010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648455"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VHS97_09000"
     CDS             complement(<1..509)
                     /locus_tag="VHS97_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448378.1"
                     /translation="MNLFKRLFTWFSGSSSPEEATISPAIGVRPPVRATAPIKKRVRS
                     FGLDAADYLPISRQEIKKAAAGRNLLANAWFGRRDLIPPADDLRTQLVDRGMVTQSLI
                     TPEQLAEIHSVGAEMDRMRPTLASVEHQVAQASEAAVASDREAQQRRKAQKKAEAAER
                     KRQRDLAIG"
     gene            complement(518..>1669)
                     /locus_tag="VHS97_09005"
     CDS             complement(518..>1669)
                     /locus_tag="VHS97_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448379.1"
                     /translation="ERDVAAGTTNVDYSWTLYEHFQSMRSYRETRFMVDPAGFEVQTD
                     QAAVVREEKIDLPPSWLRGFLQIQSAMSLPSRRVPIGRGGLYNILAYLKRHRASRSPR
                     AVRFELETWRPPIAVLEPWEERIRVGDEPYAGPRSEIIRVWGRDRLQLLSRLLPLMDG
                     AEVYLLGSGLPSFWSIRLGPIRLLLGLSGWTANDWTGSSALDQLAPPAEPDPAMMTSI
                     ARAFHGEPAMSFPRLHAVTNAPAPALAFGLNRLALLGQVIHDLPLGLYRWRQIMPVTL
                     SAEVIGPEHPETEAARRLVADHKVRVNRDETRPGGHRVIEGRVSSTDVSLLADGDGRM
                     VRGHCTCSHHFKGGLRRGPCRHLQALRTAANAITTQPTTIANWYSSLWN"
BASE COUNT          311 a          521 c          538 g          299 t
ORIGIN      
        1 cagccaatgg caaggtcgcg ctgtcgtttg cgctcggcgg cttctgcctt cttttgtgcc
       61 ttcctccgct gctgggcctc gcgatcggac gcgacggccg cctcactggc ctgggcgacc
      121 tgatgctcga ccgatgcgag ggtcggcctc atccggtcca tctcggctcc gacggagtga
      181 atttccgcca attgctccgg ggtgatcagg ctctgcgtga ccattcctcg gtcgacaagc
      241 tgagtacgaa gatcgtccgc gggaggaatc aagtcgcggc ggccaaacca ggcgttggcc
      301 aggaggttac gacctgcggc cgcttttttg atttcctggc gcgagatcgg cagatagtcc
      361 gccgcatcga ggccgaagga tcgaaccctc ttcttgatcg gcgccgttgc tcttaccggc
      421 ggccgcacac cgatcgcagg ggagatcgtt gcttcctccg gagaggatga gccggagaac
      481 caggtgaaga gccgcttgaa cagattcatt gccgctttca attccaaagc gagctgtacc
      541 agttcgcgat tgtcgttggc tgggttgtga tcgcgttggc agcggttctc agagcttgca
      601 aatgccgaca agggccgcgt ctcaaaccgc ccttgaaatg gtgcgagcaa gtacaatgtc
      661 cgcgcaccat gcggccgtcg ccgtcggcca agagcgagac atcggtcgac gacacgcggc
      721 cctcgatgac gcggtggccg ccggggcgag tttcgtcgcg attgacgcgc accttgtggt
      781 cggcgaccag tcgcctggcg gcctcggtct cggggtgctc cgggccaatc acttccgccg
      841 aaagcgtcac tggcatgatc tggcgccacc ggtaaaggcc gagcgggagg tcatggatga
      901 cctgccccaa aagagcgagc cggttgagcc caaatgccag cgccggggcc ggtgcgttcg
      961 tcacggcgtg taggcgcggg aacgacatcg ccggctcccc atgaaacgca cgcgcgatcg
     1021 acgtcatcat cgccggatcg ggctccgccg gtggagcaag ctgatcaagc gcagacgagc
     1081 ccgtccagtc gttggcagtc cagcccgaca ggccaagcaa gagcctgatg gggcccaggc
     1141 gtatggacca gaaactggga aggccgctgc ccaggagata aacttcggcg ccgtccatga
     1201 gcggcagcag ccgggacagg agctgaaggc gatctcggcc ccaaacgcga atgatctctg
     1261 accttggtcc cgcgtacggc tcatcaccaa ctcggatacg ctcttcccat ggctcgagca
     1321 ccgcgatcgg cgggcgccag gtctcgagct cgaatcggac cgcgcgcggg ctccgggaag
     1381 cgcgatgacg cttgagatag gcaagaatat tatagagccc cccgcgaccg atcggcacgc
     1441 ggcgtgacgg caggctcatt gcggattgaa tttgtagaaa cccacgcagc caggagggcg
     1501 gcaagtcaat cttttcttcg cgaacgacag ccgcttggtc ggtctgaacc tcgaagcccg
     1561 ccggatcgac catgaaccgc gtctcgcggt agctccgcat cgattggaag tgctcgtaca
     1621 acgtccagga gtaatcgacg ttcgtcgtgc ctgcggcgac gtcacgctc
//
LOCUS       DATAQL010000527         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3105617, whole genome shotgun sequence.
ACCESSION   DATAQL010000527 DATAQL010000000
VERSION     DATAQL010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105617"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..2183
                     /locus_tag="VHS97_09010"
     CDS             249..2183
                     /locus_tag="VHS97_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX3448380.1"
                     /translation="MPWKFAIASTARGLVFAGFSLLMIATNTMIPRADAASLPKQALR
                     GIDGRRIDLSAPAHGAMAIVFYSTECPISNSYSPTLATLADAFHDKPVKWLGVCVDPD
                     LSDSEVAGHARDFGLKFPVVHDRYGSLGRKLGAKVTPESFVIDDTGKIRYHGRIDDQF
                     AARRQRNANFSATELKDAIVAVLTGKAVTTEHVEAVGCPLPEIPKSAARPTYAKDVAP
                     ILQKNCQECHRPGQVGPFSLETFDQARKRASDIVAVVENRSMPPWKAAPHFGVKFKDA
                     RSLSEDEIATLVAWSEADAPEGNPADLPLTPKFPDDWQLGTPDLVVDIGADFAVPAGG
                     DDIYRCFVVPTHLDKDQYVAAVEYRPGNRSVVHHMLAYVDISGKARERDQAEPGPGYT
                     CFGGPGEPIHGGLGGWAPGNLPSFLPDGIGRSLPKQSDVIVQVHYHPRGKPETDRSKI
                     GLYFSKKPVKQVMHWGFAINPGLELPPGQSNIEVKAAWEVPVDVTAHAVTPHMHLLGR
                     DMLMSVKFPDGHVQDLIKIPDWDFNWQYTYHFEQPLDIPKGSVVYLVAHYDNSDSNPR
                     NPHKPPVLVKWGEATTDEMCIGFIGLTKKGQDLTKPGEKDDFIDILRQQEHELREKYE
                     KKLRDHPKPDKPRAGADGGK"
BASE COUNT          524 a          757 c          731 g          508 t
ORIGIN      
        1 cgcgagcaga gcgatcgaga ggccgacgaa gctgattctg gaacagattt gaatcgggtt
       61 atagcagcca gccaaggatt gtgccagata tcattaacgt acccggctct agtgggtccg
      121 ggccttcgag ttggccttga cccgagcccc cggattggtt tgactgaaca ttgttaaccg
      181 tgaagaggag agcaacgttg gagtcgcccg cgaccgcggg aaacggattg catcgaggac
      241 gtcgcatcat gccgtggaaa ttcgccattg cttcgacagc ccgcgggctt gttttcgcgg
      301 gcttttcgtt gttaatgatc gcaacgaaca ctatgattcc gagagccgat gcggcatcgc
      361 ttcccaagca ggctctccgc ggcatcgatg gccggcggat cgacctctcc gcaccggccc
      421 atggcgcgat ggcgatcgtc ttctactcga cggaatgtcc gatctccaat agctacagcc
      481 cgaccctcgc gactttggcg gatgcattcc atgacaagcc ggtcaagtgg ttgggggtct
      541 gcgtcgatcc cgacctgagc gacagtgagg tcgcggggca cgcacgggat tttggcttga
      601 aattcccggt tgtgcacgac cgctacggtt cgctcggccg caagctcggg gccaaggtca
      661 cgcccgagtc gtttgtgatc gacgacacgg gtaagatccg ttatcacggg cgtatcgacg
      721 atcagttcgc tgctcggcgt cagcgcaatg ccaacttttc ggcgaccgag ctcaaggacg
      781 cgatcgtggc ggtcttgacc ggaaaagcgg taacgaccga gcacgtcgag gctgtcggct
      841 gcccgcttcc cgagattccc aagtcagcgg cccgcccgac atacgctaag gacgtcgcgc
      901 caatcctgca gaaaaactgc caggaatgcc atcggccggg ccaggtcggt ccgttctcac
      961 tcgagacttt cgatcaagcg cgcaagcgtg cctccgacat cgttgccgtg gtcgagaacc
     1021 ggtcgatgcc cccatggaaa gcagcgccgc atttcggtgt aaaattcaag gacgcgcgca
     1081 gtttgagcga ggacgagatc gccaccctgg tggcctggtc cgaggccgat gccccggaag
     1141 gcaacccggc cgacttgccg ttaacaccca aattccctga tgactggcaa ctgggcacac
     1201 ctgaccttgt agtcgacatc ggtgcggact tcgcggtccc ggccggcggt gacgacattt
     1261 atcgatgctt tgtcgtccct acccatctcg acaaggatca atatgtggcg gccgtggagt
     1321 atcgtccggg caaccgcagt gtcgtccatc acatgcttgc ttatgtcgat atttcgggca
     1381 aagcccgcga gcgcgatcag gccgagcccg gtccgggtta cacttgcttt ggcggaccgg
     1441 gtgaaccgat tcacggcgga ctagggggtt gggctcctgg taacttaccc tctttcttgc
     1501 ctgatggaat cggccgatcg ttgcccaaac agagtgacgt gatcgtccag gtccactatc
     1561 acccgcgcgg caaacccgaa accgatcgca gcaagatcgg gctctatttc tcgaagaagc
     1621 ctgtcaagca ggtcatgcac tggggctttg caatcaaccc cgggctcgag cttcccccgg
     1681 gccagtcgaa tatcgaggtc aaggcagcct gggaggttcc ggtcgatgtc acagcccacg
     1741 ccgtgacacc gcatatgcac cttctggggc gcgacatgct catgtctgtc aaattcccgg
     1801 acggtcacgt ccaggacttg atcaagatcc ccgactggga cttcaactgg cagtacacct
     1861 atcatttcga gcagccgctc gacatcccca aaggatccgt ggtttacctg gtcgcgcact
     1921 atgacaactc agactcgaac cccaggaatc cgcacaaacc ccccgtcctc gtgaaatggg
     1981 gcgaggcgac gaccgatgag atgtgcattg gtttcatcgg cttgacgaag aaggggcagg
     2041 acctcaccaa gcccggtgaa aaggacgact tcatcgatat cctgcgccag caagagcacg
     2101 aactgcggga aaagtacgag aaaaagttgc gcgatcatcc caaacccgat aaaccaagag
     2161 ctggcgctga cggcgggaaa taatctgtcc aggggaaacc caccgttggg aattactggt
     2221 attattgctg gatttactca atcgttacca tgcattctac cgattcgatc tgacagcgtt
     2281 ccctagtcac tccgaaacgc attgccgttc tgcgacgcgc cgcgttcgcg ccacactttc
     2341 tcgtccgctt cgccgtgtcc aaggatgaca gatcgattcg actgttcgcg ctgcgcccgg
     2401 ctgttgcgcg tgttttactt gggcatgact tcagcgacgg cacggtcgca ggagaccgat
     2461 cctcgggagg atcgctagtt gcgagcgacc ggcgacttcc gagatacgcc gatccgccct
//
LOCUS       DATAQL010000528         5097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1885063, whole genome shotgun sequence.
ACCESSION   DATAQL010000528 DATAQL010000000
VERSION     DATAQL010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885063"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..1394)
                     /locus_tag="VHS97_09015"
     CDS             complement(324..1394)
                     /locus_tag="VHS97_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX3448381.1"
                     /translation="MPSRSPPLGGFVLVWRNWVLRLLERIYWIGTFIGSLTLLVCGGF
                     VPVARGWLRNEIDSWSDIVGALGGIWFRDQTNDPDSDLGPVLARVDAPLLFSTIEKVG
                     RRLGVKPPSQVRLTYLPCCGVVAWGRSRALLIGLPLFRVLTQGELRAIVAHELAHLAR
                     GDATAAARSARFVEALEQAVERGGHRIRGPLGAWARYCLREASWLIEPVARGQEARAD
                     RCAAAIAGGSAAASALVKVAVVQPLFRELLAAYDPDESQEMSLYAYFRAFWYRLPADV
                     HTAMRLGVLTSRQGANDPAHPPLPDRISALQAYPDPASLNGDTLPATTFLGDLEIFEQ
                     MLHNRLFGLPVVEPSVFHRAGS"
     gene            complement(1971..2471)
                     /locus_tag="VHS97_09020"
     CDS             complement(1971..2471)
                     /locus_tag="VHS97_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448382.1"
                     /translation="MRLHRKLTAILAASLALVVWGCNSEQVESGADATAKGLDKTGGA
                     LESAGKKLGEKIEHAGEGTKLEKAANATGAGLEKAGEKTHEALDAAGDKIKEAAPAVG
                     KAVDKAGDKLKDMEHKAVDKAKDVGKAIKEEAGDLKEKAGDKFKELKDKASDALHKDK
                     APGSGS"
     gene            3181..5016
                     /locus_tag="VHS97_09025"
     CDS             3181..5016
                     /locus_tag="VHS97_09025"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3448383.1"
                     /translation="MPIVAGEPGSSLRAETDELDLPGSTGARISGRVYDERGNAVSNA
                     KVRLAVSSSPGGKVVYDTTVRDGSFTLRGLRAGLSYIVIAEYQGEDGIVTGRVQAKAP
                     DGDVRISLQPRDQTGQGHASIQPARPRVEPISNVDPVDDDPPADSRPKAAPVNSEDLE
                     LPAPEAASIPARRNVQLSRASADSSSAPVRAGWNPRQTGTPNAAPATASTHEPSNDRT
                     SISRPPAAGDSTPQADDDGPNPLPPALESGAAGSKHSAVRVDDDPVRVARVPSGSARR
                     KSRPRGTVSLERDDSRSIGILDPSSEREPRSMPEELVPGGRVITPGSSGPISVDEPPD
                     AVAPGDSSASRSQQPAWPTAGQAANENSLETTGSSDTRVAAPTPAKRPTWRELAGNQS
                     DLPVDESVRRASGDAEQTRDHAVVTLTSSTTRNARPAKARLLGGSRPLVDPAIQKTVC
                     RFDPSERRLVDFQLPNLDGQMVSLHDLSADVILLDFWGSWCEPCRTSIPHLAELQAKM
                     GRNRLQVIGIACEQGPTLQDRRAAAAKAIKELGINYPVLLTNKDGDCPVQKALQVQFY
                     PTLVLLDRDGRLLAREHGATEITLARMDRAITAALRGSNSARVID"
BASE COUNT         1011 a         1628 c         1525 g          933 t
ORIGIN      
        1 cgacggcctt gatgatcttg ccctcgatca gcaccatccc gttcgggacg agttgatcgg
       61 aagtgccgtc aaaaagtcgg gctgccttga gtatgaccgg ctgtggccgc ggcgatggtt
      121 cggcctgcgc atggagaaga aataaaacgg ggacgaggct gatcgggatc atcacctggg
      181 tggctccgct acttgtgggt cgaatgagag ttcgcggaaa cgtgaattaa aaaacaaacg
      241 cagcgcgggt cgctgagcgt gcgccgcgat ttttttgagc ccaaagacgc cccgtgcatg
      301 agccgcatac ggccgcatgg caatcacgaa ccggcgcggt gaaatacact gggctcgacg
      361 acggggaggc cgaacagccg attgtgcagc atttgttcga agatttcgag atcgcccaag
      421 aacgtcgtcg ccggcaaagt gtcgccgttc aagctcgcgg ggtccggata agcttgaagc
      481 gcggaaatgc ggtcgggcag gggggggtgg gccggatcgt tggcaccctg tcggctggtc
      541 agaacgccca gccgcatcgc cgtgtggacg tcggccggta ggcggtacca aaacgcgcga
      601 aaataagcgt agaggctcat ctcttgcgat tcgtccgggt cgtatgccgc gagaagttcg
      661 cggaagagag gttgaacgac agcgaccttg accagcgcgg acgctgcggc gctaccaccg
      721 gcgatcgccg cggcgcaacg atcggcccgt gcctcttggc cgcgggcgac tggctcgatc
      781 aaccatgagg cttctcgcaa acaatagcga gcccaggcgc cgagaggtcc tcgaatgcgg
      841 tgtccaccgc gctcaaccgc ttgctcgagc gcctcgacaa accgagccga ccgagccgcc
      901 gcagtagcgt cccccctcgc cagatgagcc agctcatgag cgacgatggc ccgcagctct
      961 ccctgggtga gcactcgaaa aagcggcagc ccgatcaaga gggcacgcga ccgaccccac
     1021 gcgaccacac cgcaacatgg cagataggtc aagcggacct gcgacggcgg cttgacgccc
     1081 aagcgacggc cgactttttc gatagtcgaa aaaaggagcg gcgcgtcgac cctggccaga
     1141 accgggccga gatcgctgtc ggggtcgttg gtctggtccc tgaaccagat ccctcccaaa
     1201 gcgccgacga tgtcggacca gctatcgatc tcgttgcgga gccaaccgcg cgcgacgggc
     1261 acaaaaccgc cgcaaactag caaagtcaac gaaccaataa acgttccgat ccagtaaatg
     1321 cgctcgagca ggcgcaaaac ccagttgcgc cacaccagga cgaaaccgcc caggggaggc
     1381 gacctgctcg gcacgtttgg catcgttcga tcggttcctc gctgactatc gccagagggc
     1441 aaccgacctc cgcatcgaat acaatgcccc gtcgactcat tggaactcac gttttcgtgg
     1501 gcgtcgagct cggcccggca gttcgggcac acggttgcgc gcgccgttct cgatccggat
     1561 cggtccatcg cctgtctccg cgcgtcgctg tcaaccgatc gctccggacg cgacattatc
     1621 ctgcgctcga caaaagcaag ttcgtctccg agttagttcg gagataagtg tccactatca
     1681 ggccaaggaa tctgcgccac cctggtcccg gcgagctcgc ggtggctgtg gcttcgttga
     1741 ggctacaccg catcttgctc gcggaaccat tcagtcacgc atgaaactgc atcttcgatc
     1801 atcgagacgc agatcgtcaa ggcttactct ggctcgaggc aaatccacgg caaacggggc
     1861 acgatcgatg cgcgacgacc tggatgcgtt ctatgccgga gtgcagggcg cctcggccag
     1921 catcgaatcc accggccgag gactacttta gaacgcttga cgtcggggtt ttagctgccg
     1981 ctgcctgggg ctttgtcctt gtgcaaggcg tcgctcgcct tgtctttgag ctccttgaac
     2041 ttgtcgccgg ccttttcctt gagatcgccg gcttcttctt tgatcgcctt ccccacgtcc
     2101 ttggctttgt cgacagcctt atgctccatg tccttgagct tgtcgccggc cttgtcaact
     2161 gcctttccaa cggccggagc cgcctctttg atcttgtcgc cggccgcgtc gagtgcctca
     2221 tgtgtctttt cgccagcctt ttcgagaccg gcaccagtcg cattcgcggc cttctcaagc
     2281 ttggtcccct cgccggcatg ctcgatcttt tcgcccagct tcttgcctgc gctctccaga
     2341 gctccaccgg tcttgtcaag tcccttggcg gtcgcatccg ctcccgactc gacctgttcg
     2401 ctgttgcagc cccaaactac cagagccaac gaagcagcca aaatggcagt caatttccga
     2461 tgaagtcgca ttgcaaacat ctccttgata aacctacgaa cacacaatga cgtaaagcca
     2521 gcacattctc gagccacatg gcgatgagga ttgcgacgac gatcaatcta gttttgatcg
     2581 ctgcctcgtc ggctctcagt catttcgacc ttcgctgaca tgcaggaaac cccggccacc
     2641 atcttgacat tagcaaactc atgtgcaccg ggctaggtgc cagagatcgc ccggcccgac
     2701 cgcgaacgat ttcgtaccca tcagatttta ttcgtgacca aggccacaat cttaaacagg
     2761 cagcaggcag tgggcagatt gacgggaaca gacggccagt cattaagtcg agtctttgtc
     2821 cgtttttcca aaaagatggg cggggagatt caagacccga tgtgggcctg gccgattcta
     2881 agcgatacgg cgcccgcgca tcgtgggccc gccggcggcg atgaaaaggc gacgagttcc
     2941 gcatcttggt tgatgtgggg gagcgatcag gatgatcgat ccttctttgc tagttacgct
     3001 ccaggaaaca accccgcgcc gtccagtcca ggaggaggat atcgcgaaat gcgcatcacg
     3061 agatcgaaac tttgcctcct gaccctcctt tgcactctcg cgggatgctc ccagaccggt
     3121 ggcgtgcggt ccggtgcctc ctcaaatatg aggacggttg cctcggtggg agacaagccg
     3181 atgccaatcg tggcggggga gccggggtcg tccctccgag ccgaaaccga tgaactggat
     3241 ctgcccggat cgacaggcgc ccggatctct ggccgggtat atgacgaacg tggcaacgcg
     3301 gtttccaatg ccaaagtacg tctggccgtg agtagttccc caggcggcaa agtcgtttat
     3361 gacacaaccg tacgcgacgg ctctttcaca ctacgcggtt tgcgtgcggg cttgtcctac
     3421 atcgttattg ccgaatatca gggagaagac ggcatcgtga ctgggcgcgt gcaagccaaa
     3481 gcgcccgatg gggatgtgcg aatcagtctc cagcctcgtg atcagaccgg tcaaggccac
     3541 gcgtcgatcc agcccgcgcg accgcgggtt gagccgatat cgaatgtcga tccggtggac
     3601 gatgacccgc cggctgacag ccgcccaaag gcagctccgg tcaactccga ggatctggag
     3661 ctgccggcgc ccgaagcggc gtcgattcct gcgcggcgaa acgtgcaatt atcgcgagcg
     3721 agtgctgaca gctcgtcggc accggtccgt gcgggctgga acccgcgaca gactggcacc
     3781 ccaaacgccg cgcccgcgac ggccagtaca catgaaccga gcaacgacag gacctcgatt
     3841 tctcggccac cggccgccgg cgattcgact ccccaggctg acgatgacgg cccaaatcca
     3901 ttgccccccg cactcgaatc gggcgccgca ggttcaaagc actcggcggt ccgcgttgac
     3961 gacgatcccg tcagggtggc tcgggttcct agcggctccg cccgtcgcaa aagccggcct
     4021 cggggcacag tgtctcttga acgggacgac tcccggtcga tcggcatctt ggatccctcc
     4081 agtgagcgcg agccacgttc gatgccggaa gagctggtcc cgggcgggcg ggtgatcaca
     4141 ccgggctcgt cgggtccgat ctcggttgac gaaccccctg atgccgtcgc ccccggcgat
     4201 tcctcggcaa gtcgctcgca acaaccggca tggccgaccg cgggacaagc tgcgaacgag
     4261 aattcactcg agaccaccgg ttcctcggac acgagagtcg ccgcgccgac tccggccaag
     4321 aggcccacct ggcgcgagct ggcgggcaat cagtccgacc taccggtcga cgagtcagtg
     4381 cgccgcgcct ccggcgacgc cgagcagact cgcgatcacg ccgtggtcac gctcacaagc
     4441 tcgacgacgc gcaacgccag gccggccaaa gctcggttgc tgggcggatc gcggccgctg
     4501 gtcgatcccg ccatccaaaa aaccgtgtgc cgctttgatc ccagcgagcg ccggcttgtt
     4561 gattttcagc tccccaacct cgacggacag atggtgtcgc tccacgactt gagcgccgac
     4621 gtgattctgc tcgatttctg gggaagctgg tgtgagccct gccgaacgtc gatcccgcac
     4681 ctggccgagc tccaggccaa gatgggtcga aatcggcttc aagtgatcgg catcgcctgt
     4741 gaacagggtc ctacactcca ggatcgccgc gcggccgcgg ccaaggcgat caaggaactc
     4801 ggaatcaact accccgtgct tctcacaaac aaagacggag actgccccgt ccagaaagcg
     4861 ctccaggttc agttttaccc gaccctggtc ttgctcgacc gcgacggccg attgctggcg
     4921 cgcgagcatg gcgcaaccga gatcacgttg gctcggatgg accgagcgat tacagcggct
     4981 ctccgcggct caaacagcgc tcgcgtcatc gactgaggac tgagaccaag gcgccgcgaa
     5041 tcatgacaga ccgacgatgc ggccttgctg atcgacgtcg atgcgctcgg cggctgg
//
LOCUS       DATAQL010000529         4875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3870498, whole genome shotgun sequence.
ACCESSION   DATAQL010000529 DATAQL010000000
VERSION     DATAQL010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3870498"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VHS97_09030"
     CDS             complement(<1..186)
                     /locus_tag="VHS97_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448384.1"
                     /translation="MDHPRYNDIMKQLDDDPGPDRDEEESTYFHQMVVGGLQPLGDVG
                     DAPSSAAGIVNVFPVPTP"
     gene            complement(265..720)
                     /locus_tag="VHS97_09035"
     CDS             complement(265..720)
                     /locus_tag="VHS97_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448385.1"
                     /translation="MALKIPSNPATMQAWVKEMIDECMASASERGMVYTRAGQYYYTG
                     AADSRAALYNKTKSFIDKLAGFLMQPTDVRLRLIYDSGEPDDILERSELVGEKLSADF
                     RQVDADVTFADAVTWSLINGCGLLKVLPDGDSGTFKVAQVHPLNFGVLS"
     gene            complement(721..1374)
                     /locus_tag="VHS97_09040"
     CDS             complement(721..1374)
                     /locus_tag="VHS97_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448386.1"
                     /translation="MMGDTSSGAGFSLQDIIGALSRMASGGGPAYPRVEPPINAPGRA
                     AGLKAVEGATTGRQEPTVSGETARAAGLSAVDKEMDAANTEAWKAKMAGRKADRARAG
                     SEGKAAVADATSPSRVRDMMTQLSQLPGWTPEESAHWARALGTVGVENALHTPDNPPG
                     WTTPLEPMPLTAPGQTFGPHAVVTPDLAGQTPYAPASQRASHYGKDAVERELDKEPD"
     gene            complement(1371..1574)
                     /locus_tag="VHS97_09045"
     CDS             complement(1371..1574)
                     /locus_tag="VHS97_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448387.1"
                     /translation="MALVALITNWWWARMRRIDLELLWPVCKREAADLDHAKAAFAVH
                     AFNDRAWLCLGQDELMAAIDELR"
     gene            complement(1581..1802)
                     /locus_tag="VHS97_09050"
     CDS             complement(1581..1802)
                     /locus_tag="VHS97_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016049872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEX3448388.1"
                     /translation="MNSFGWAIEQLEDGERVSRSGWNGKGMWLELQKPDKNSKMTLPY
                     IYMSTAQGDLVPWLASQTDMLAKDWDLTE"
     gene            complement(1829..2290)
                     /locus_tag="VHS97_09055"
     CDS             complement(1829..2290)
                     /locus_tag="VHS97_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448389.1"
                     /translation="MINRTYMCPECNYRMEQTLTKEQWDQPPPSCEACDQRMNQEFSP
                     PAIGGSIGGKARALAEDIIANDYHVADIRFNNRQGETSKVRYKDQTASVLPSDWQKAG
                     HAAMLQTAIGIGQQNRREFGMDGLDMLKRGIASGTQVDLIEASKRRAIKVW"
     gene            complement(2399..2617)
                     /locus_tag="VHS97_09060"
     CDS             complement(2399..2617)
                     /locus_tag="VHS97_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448390.1"
                     /translation="MNGLAQRLDIAFNGDAKGKARKTGFVLLVFPFGDNDGRANYISN
                     GADRRDIVTLMKEQIRRFEGQPEMEGRA"
     gene            complement(2671..4488)
                     /locus_tag="VHS97_09065"
     CDS             complement(2671..4488)
                     /locus_tag="VHS97_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448391.1"
                     /translation="MATKPSIRQGIYDLLGAVEIDTKEEGRTHVDPWMSQRMVIDAVA
                     KGLDEGVHEFVVLKCRQVAITTVCSVIELFWALANPGVQGAIIADRTDNLERLRRIFA
                     ALLETLPPEWRSTEHRLTQNNRNGMAFANRSVIDLMAAASNPDLGASRALNMMHATEC
                     GTWKSLAGVESLKASLARVNPTRLYVWESIANGFNWFYNHCQQAKVDRHMRFIFVGFW
                     ANPTYSIPKSDPDYKTFWDGGRLTDEEIEKARFVKRTYGVTVKPEQIAWWRRESEFRA
                     EEYMLRHYPWNEKECFVASGSSFFPAQRTLELREALAEGPFYQGYRYSFEDDFLGSKI
                     EQTTNKDEVMLRVWEPPEPGGVYAIGGDPSGGGGGDADAHAIEVFRCYADRLVQVAEF
                     QSNRPLTYQFAWVLAHLCGAYKEHIANIEVTGVGAAVIPEVRNLRQLAQRGIIQAEPG
                     TENILNMIGAVRWFLYKRADTLGGAGNVIAWKTNQDNKAMVYTALRDSLMKKKTEPEI
                     EIRSIRLVKELQAIIDDDGWLGAGPDTGENDDLVSATVLAHHTWVDMLRNGLIARNLT
                     WDSVKGDRPPQNAGTVLNFAFSEHIRKINQRANRHVEKF"
     gene            complement(4472..4690)
                     /locus_tag="VHS97_09070"
     CDS             complement(4472..4690)
                     /locus_tag="VHS97_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448392.1"
                     /translation="MPKAKAYTTEPDANNHQLDPVIDGLLDRLPAPGDVFLPADRQLW
                     LKIMGDVFQLIYLDKEPEPEPSTDGDQT"
BASE COUNT          784 a         1566 c         1497 g         1028 t
ORIGIN      
        1 cggcgtcgga acgggaaaga cgttgacgat cccggccgca gaggacggcg cgtcgccaac
       61 gtctcccaac ggttgcagcc cgcccacaac catttggtga aagtaggtcg attcctcctc
      121 gtcgcgatcc ggccccggat cgtcgtcgag ctgcttcatg atgtcgttgt agcgcggatg
      181 atccatgagc gaggtccgca gccgcgactt gctcggatag ctgacgtgac ataacgcctc
      241 ttgctcgtcg agatgcagcg tcgtttagga caggacgccg aagttaagcg ggtggacttg
      301 cgccaccttg aacgtgccgc tgtcgccgtc aggcagaacc tttaacaggc cgcagccatt
      361 gatcaggctc cacgtcacgg cgtcggcgaa ggtgacgtcg gcgtcgactt ggcggaagtc
      421 ggccgacagc ttctcgccca ccagctcgga gcgttcgagg atgtcgtctg gctcgccgct
      481 gtcatagatc aggcgcagcc ggacatcggt cggctgcatc aaaaacccgg caagtttatc
      541 gataaatgat ttcgtcttat tatatagtgc agcgcgacta tctgccgctc cagtgtagta
      601 atattgccct gcgcgagtgt agaccattcc tctttcacta gcagaggcca tgcattcatc
      661 aatcatttcc ttaacccatg cttgcatggt ggcggggttg gacgggattt tgagcgccat
      721 ctagtcgggc tctttgtcga gttcgcgctc caccgcgtcc ttgccgtagt gcgaggcgcg
      781 ctgcgacgcg ggcgcatagg gggtctggcc agcgaggtcc ggcgtcacga cggcgtgcgg
      841 cccgaaggtt tggcccggcg ccgtcagcgg catcggttcg agcggcgtcg tccagccggg
      901 cgggttgtct ggcgtgtgca gcgcgttttc gacgccgact gtgcccaagg ctcgcgccca
      961 atgcgcgctt tcctccggcg tccagcccgg caactgcgac agttgcgtca tcatgtcgcg
     1021 gacgcgcgac gggctcgttg cgtcggcgac ggccgccttg ccttccgatc cggcccgcgc
     1081 ccggtcggcc ttgcggcccg ccatcttggc tttccaagcc tctgtgttcg ccgcgtccat
     1141 ttccttgtcg acggcactga gccccgcggc gcgggctgtc tcgccgctca ccgtgggctc
     1201 ctgcctgccg gtggtcgcgc cttcgacggc cttgagcccg gcggcccggc cgggcgcgtt
     1261 aatcggcggc tcgacacggg gataggcggg tccgccaccg ctggccatgc gagacagcgc
     1321 gccgatgatg tcttggaggc taaagccagc gccgctgctc gtgtcgccca tcatcgcagc
     1381 tcgtcaatgg cggccatcag ctcgtcctgg cccaagcaga gccaagcgcg gtcgttgaac
     1441 gcatggacgg cgaacgccgc tttggcgtgg tcgaggtcgg ccgcctcgcg cttgcagacc
     1501 ggccacagga gttcgaggtc gattcggcgc atccgcgccc accaccagtt cgtgatcaga
     1561 gccaccagcg ccatgtcact tcactcggtg aggtcccaat ccttcgccag catgtcggtc
     1621 tgcgaagcga gccacgggac aagatcgccc tgcgccgtcg acatgtagat gtagggcaag
     1681 gtcatcttgg agtttttatc cggcttttgg agctccagcc acatgccctt gccgttccag
     1741 ccggatcgcg agacgcgctc gccgtcttca agctgctcga tcgcccaccc aaagctgttc
     1801 atgtcacccg cctccatcag gcttcgctct accagacttt aattgcgcgc ctcttcgatg
     1861 cctcaatcaa atccacttgc gtcccgctgg caatcccgcg cttgagcatg tccagcccat
     1921 ccatgccaaa ctcgcgccgg ttctgctggc cgatgccgat ggcggtttgc agcatcgccg
     1981 catggccagc cttctgccag tcgctcggga ggacggaggc ggtttggtcc ttgtagcgga
     2041 ccttggacgt ctcaccctgc cggttgttaa atcggatgtc ggcgacgtgg taatcgttgg
     2101 cgatgatatc ctcggcgagc gccctagctt tgccgccgat gcttccgccg atcgccgggg
     2161 ggctgaactc ctgattcatc ctctgatcgc acgcttcgca gctcgggggc ggctgatccc
     2221 actgttcctt cgtcagcgtt tgctccatcc ggtagttgca ctccgggcac atgtacgtcc
     2281 ggttgatcat tgtcgagccc cagatgtttg aggacgcgcg ccatgtcgct ttcgagcgtg
     2341 gcgattcggt cctcggtttg cgcggtgcgc agcatcatcg gcgagcgggg cagaggcatc
     2401 atgcgcgccc ctccatttcc ggctgtccct caaatcgcct gatctgttcc ttcatcagag
     2461 tgacgatgtc gcgccgatct gcgccgttcg agatgtagtt cgcccggccg tcgttgtcgc
     2521 cgaaggggaa caccagcagc acgaagccgg tcttgcgcgc tttgcccttg gcgtcgccgt
     2581 tgaaggcgat gtcgagtcgc tgcgcgagcc cattcatcaa ctcgcgatat ttggcctcaa
     2641 tcggtgcgtc gcctagccgg ttatcgttca tcagaacttc tccacatgcc ggttcgcgcg
     2701 ctggttgatt ttacggatat gctcgctgaa cgcgaagttt agcacggtcc ccgcgttctg
     2761 cggaggccgg tcccccttca ccgaatccca cgtcagattg cgagcgatca acccgttgcg
     2821 cagcatgtca acccaagtgt ggtgcgcaag cacggtcgcc gagacaaggt cgtcgttttc
     2881 cccggtgtct ggcccggccc caagccaccc gtcgtcgtcg atgatcgcct gcaactcctt
     2941 caccagccgg atcgagcgga tttcgatttc cggctcggtc ttcttcttca tcaggctgtc
     3001 ccgcagcgcg gtgtacacca tcgccttgtt gtcctgattg gtcttccacg cgatcacgtt
     3061 gcccgcgccg cccagcgtat cggccctctt gtagaggaac cagcgcacgg cgccgatcat
     3121 gttcaggatg ttttcggtgc ccggctcggc ttgaatgatg ccgcgctgcg cgagctggcg
     3181 gaggttgcgg acctcgggaa tgaccgccgc cccgacgccg gtcacttcga tgttggcgat
     3241 gtgttccttg taggccccgc acagatgggc caggacccaa gcgaactggt aggtcagcgg
     3301 gcgattcgat tggaactcgg cgacttgcac gagccgatcg gcgtagcagc ggaacacctc
     3361 gatggcgtgg gcgtcagcgt cccccccgcc tccgcccgac ggatcgccgc cgatggcgta
     3421 cacgcccccc ggctccggcg gctcccacac gcgcagcatc acctcgtcct tgttcgtcgt
     3481 ctgctcgatc ttggagccaa ggaagtcgtc ctcgaagctg tacctgtagc cctgatagaa
     3541 cggcccctcc gctagcgcct ccctgagttc gagggtcctc tgtgcgggga agaaagacga
     3601 gcccgaagcg acaaagcatt ccttttcgtt ccacggataa tgtcggagca tgtactcctc
     3661 ggcccggaac tccgattcgc gtcgccacca tgcgatttgc tccggtttga cggtcacgcc
     3721 gtaggtccgt ttgacgaagc gggccttttc gatttcctcg tcggtcaggc gcccgccgtc
     3781 ccaaaaggtt ttgtagtcgg gatctgactt ggggatcgag taggtcggat tggcccagaa
     3841 gccgacgaag atgaaccgca tgtgacggtc gaccttggct tgctggcagt gattgtaaaa
     3901 ccagttgaag ccatttgcga tcgactccca gacatagagc cgggtcgggt tgacgcgggc
     3961 cagcgaggcc ttgaggcttt caactcctgc tagagatttc caagtgccac attccgtggc
     4021 gtgcatcatg ttgagcgcgc ggctcgcgcc aaggtcgggg ttcgaggccg cagccatgag
     4081 gtcgatcacg ctgcgattgg cgaacgccat gccgttgcgg ttgttctggg tcagccgatg
     4141 ctcggtcgag cgccactcgg gcggcagggt ttcgaggagc gcggcgaaga tgcgccgcag
     4201 ccgttcgagg ttgtccgttc gatcggcgat gatggcgccc tgcacccccg gattggcgag
     4261 cgcccaaaac aattcgatca cgctgcaaac ggtggtgatg gcgacttggc ggcacttgag
     4321 gacgacgaac tcgtggacgc cctcgtcgag ccccttggcc acggcgtcga tcaccatgcg
     4381 ctggctcatc cacggatcga cgtgggtgcg gccctcttcc ttggtgtcga tttcgaccgc
     4441 gcctaagagg tcgtagatgc cttgccggat gctaggtttg gtcgccatcc gtgctgggct
     4501 ccggctccgg ctccttgtcg aggtagatca actgaaacac gtcgcccatg atcttcagcc
     4561 agagctgccg atcggcgggc aggaacacgt cgccgggcgc gggcaggcgg tcaagcaagc
     4621 catcaatgac cgggtccagt tgatgattgt tggcgtcagg ctcggtggtg taggccttgg
     4681 ctttcggcat cagctcctcc caaaaactga cctgtcgcat gttcagtcca tcgggacaat
     4741 cgcgttgatc gagccggtgc tattgctcgt ggaggaaccg acgccccaca ccgtccacac
     4801 ttcaccgttc ggcgtaatct cccaaatcga atacgcccat gtgaggccgg tcgggccgcc
     4861 aacgacgacc ccgct
//
LOCUS       DATAQL010000530        12289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4175192, whole genome shotgun sequence.
ACCESSION   DATAQL010000530 DATAQL010000000
VERSION     DATAQL010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 12289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12289
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4175192"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VHS97_09075"
     CDS             <1..1167
                     /locus_tag="VHS97_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3448393.1"
                     /translation="LLKSEFGLSQRQLGYLGSAFMLVYALTSPFSGYTVDFLPRRLLI
                     TLGLTFWSLICAATALSRSFLQLIFFRAAEGLGESFYFPASMSILADYHGGRTRSRAL
                     GIHQTSVYLGTAGGAVLAGYLGERLGWRSPFWVLGLAGVAYAVLLGFVLIEPRRESTD
                     RDEARRHEEADVPWNQSPPSSDSLAMKLGRILTNPAAALLLAVFCGANFVAATFLTWL
                     PSYIFERFELGILSSSLTSTFWPLASLPGALMGGVAADLSSRRGRGGRIRVQSVGLCV
                     AAPFVFLTGWATSVPVLIAALIGAGICKGIYDSNIFASLFDVIRPEDRGTAAGLMNTV
                     GWTGGFAAPTVVAIASERLGLGATIAATAAVYLLGGMLAYGAARMAETRHFRMA"
     gene            1200..2885
                     /locus_tag="VHS97_09080"
     CDS             1200..2885
                     /locus_tag="VHS97_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX3448394.1"
                     /translation="MNSGSLSKSSTESGYVWARRGDVNAFFGLMLDNIGGMILMASLL
                     VLGFGMPRSFVLTRMIPGTAVGVLVGDLIYTFMAFRLARRTGRSDVTAMPLGLDTPST
                     FGAVFLIIGPAYQAALGRGLEPDLAARHAWFLGISMLVASGIFKLACAPWSGWIRNRV
                     PRAGLLGSLTAIALVLISFLPLMDIAGEPVAGLVALGLMLATLTARWQFPGRFPGALG
                     AVVIGCLLYYGMHLVGWGPGSGSEGAAPAVALRAVLPVPVEDWLAWIGGAWSEAVGYL
                     PVAIPLALATVVGGIDCTESAAAAGDDYPTGQIIAAEGLATLVGGIFGGVIQSTPYIG
                     HPAYKAMGARSAYTLATAIFVGAAGIFGYFDWIFFIIPKAVIFPILIFIGLEITSQSF
                     HATPARHYPAVALACVPALAYLALLTLNQLMPELGKPFAELRPQTQHWIQTVTVVAGG
                     GGFIVTSLLWGTALAHLIDGRVRPLVASLLLAAVFSWFGIIHSPLPSSPIMSPGAVIR
                     QLEAEGRAAASARQTPYHWSAGYVAMALALVALSRFGTLPAFTELEAHKPVSG"
     gene            3296..5065
                     /gene="ptsP"
                     /locus_tag="VHS97_09085"
     CDS             3296..5065
                     /gene="ptsP"
                     /locus_tag="VHS97_09085"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX3448395.1"
                     /translation="MRKGIGVSPGVVVGVAHRVESALGAIEQETLDHAGLIAAEVERF
                     ALAVAESATELETVVQKVADVLGAAEADIFKSHLQIVNDPALLSKVRALIENQQLTAL
                     SALQVVMNGYVAQLARSQQELFRERMTDIRDVILRIESHLTRKKIAHVDPAGDSRNGD
                     ESVILVAHEILPSQAMSLGDLPIAGIVTELGGSSSHSAILARSRGIPAVSGVEGIMSE
                     VESGDLMIVDGRDGMVLVRPDQEATSAYRKVQREFFNFKDRLIANRDLPAKSADGTHV
                     ELLANINNLPDAQAAHKVGATGVGLFRTEYLFLTHHDVPGEEEQYEYYRQIILNSPNQ
                     TITIRTLDLGGDKTVPYLGRRGEPNPFMGWRSTRIFFENPKLFVTQIRAILRAGRHGK
                     VSMLFPMITTLEELLFVNNLVKETRNNLRREGVAFGEDVKTGVMVEVPAAAVCIDAIL
                     REIDFISIGSNDLIQYLVAADRDNPKVAHLCEALSPAIFRVLRMVLDACQRTGTPVTL
                     CGEMAGQPRSVLVLFGMGLRRFSMSPAFIPTVKELLSSVTTAQSERFAHHVLQLSTSE
                     EIRSYLTARLHEVSSTLEVFDSS"
     gene            5438..6685
                     /locus_tag="VHS97_09090"
     CDS             5438..6685
                     /locus_tag="VHS97_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX3448396.1"
                     /translation="MAKRPSVKEILEAARKGGPAKPADESAPAAEAAAEETAPVEDVG
                     DVAVAAEPAGAPAPAANVPTAASLGRPLTLKEKLAAARAGGAPAAAAAPAPVKKAAPA
                     PAAATGGPAEEVKAVPPPAKPLGRPMTLQEKLAAARGTAAAAGSAAPASGAKAAAPKP
                     AAPKAEQARTLPPLGEITDPKDLAEAARQAAAKKAKEIAAKAAAAAPPKAAAKKGPAK
                     PQTVPPRPTRELAGATAAAEPRFNRRGFFLGSVFVTWAIAWIVVAWATFTAALSAMTL
                     MSVRFMFPNVLAEPPSTIKVGLPTNYEPDEVSERFKAEWGFWIVRSTRYNGQDVIYAL
                     KSVCTHLGCPPNWLSAEQKFKCPCHGSGFYISGINFEGPAPRPLERFKVALADDGQIQ
                     VDKSQIFQQELGQWSDPDSFVPA"
     gene            6774..7547
                     /locus_tag="VHS97_09095"
     CDS             6774..7547
                     /locus_tag="VHS97_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX3448397.1"
                     /translation="MPILDKITDNQLWKSIFRHPMPTDRRNRVVVMLTNFFLHLHPVS
                     VRKQGIALSYTWCMGGTTFFLFIVETVTGVLLMFYYRPTLEHAYNDILALRDVTTLGI
                     LRELHRWGAHAMVIAVWLHMYRVFLTGSYKPPREFNWGVGVILLVLTLLLSFTGYLLP
                     WDQLAIWAITVGSNMARATPGLGVEGPGASLLKLNGVSLITSGSDAKFALLGGRSVGE
                     MTLNRFYVLHCVAIPLAAALLIMIHFWRVRKDGGISGPM"
     gene            7699..8589
                     /locus_tag="VHS97_09100"
     CDS             7699..8589
                     /locus_tag="VHS97_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448398.1"
                     /translation="MHNDLTVGVMAGLAWYYLLAAALNAAAAARISYGELVSEGASRI
                     GLAPKTRHFPAWLLVAFLGLYSAAVLMILGRGVLPGSVGVAYGLCALANGLLAVMAGA
                     DAAHFAEIKAHGHGEGDSLSPPSLDDHHPAVGLGKPINRTLWTLIWGFVAVLFQAMGV
                     AYYLSAAAQTGNSDQTPVTGILMPQFMRDAIDFAAGPTTFFVGATIGFIAMIRWRRFM
                     ANGLVGWAIVNLFLLYFGLSMTDYDFRDIVTKPDNVPIVGLLILVGFFFWFGLRRAVI
                     NDAGPPRACPRSRTSSPTRP"
     gene            8550..9323
                     /locus_tag="VHS97_09105"
     CDS             8550..9323
                     /locus_tag="VHS97_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448399.1"
                     /translation="MPTLEDLEPDKTLTWPDLVYTELICMVLLTIFLVVWGIALQAPL
                     EQPASSTVAPNPSKAPWYFLGLQEMLVYFDPWMAGVVLPSMIIVGLMAIPYIDTNKSG
                     NGYYTYNDRKFAYLTFQYGFVILWVVLILLGTFLRGPNWNFFGPYEFWDLHKLIPLNN
                     VNLSDIVWIQLLGTAKPSNILVREFPGIALTLAYFTIAPYLLYRLFFKKYAEQSGTLR
                     YVTLAVLLLFMASLPIKMVLRWTINLKYVVAIPEYFFNI"
     gene            9400..>12289
                     /locus_tag="VHS97_09110"
     CDS             9400..>12289
                     /locus_tag="VHS97_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3448400.1"
                     /translation="MPASEETYRRQPTLHLVFAISSVAMLLSTVWMVMADHLRPWKEV
                     QRKFQSVERDKLEAAEKEKLEEQKQKYQTQLDDIDAKIQAAEADADTRARDLRVIDNE
                     LKVLEGRTEKIDTAKRFQKAELDSKRSLYDGMIDRGEEPAARRYLTTVVKDAEDRLKS
                     LSVDLEKAQETLKSKKDTKEKLLGFVDDLKKEKDRLTREADRVTRVIEQKDALYGGTL
                     HWYSQPMAFLRGLPGIDLMPPTKIQQISLPELTINYNFKEVPRYDRCATCHQGIDRIG
                     YDKNAHGEEMEEVFRSHPFLTTGATTLDPRGKVVTAGLYLDANGPHPVNSFGCTICHG
                     GQGSGTDFTYASHTPDSREEGKKWEEEYNWHEIHHWDFPMLPRRFIESGCVKCHTQVT
                     DIPQAKKLQAGYQRIVKFGCTGCHTIGGDGSYGPDLTDERQVGPNLSHIAAKDAQEWV
                     KKWIANPHAFRPDSRMPRFYGLTNNGATEDWPKNYAEIHAITHYLFAKSTPPDNFTDP
                     PAKSDPAKGKELFLQKGCLACHQHRPYQVADIQLGDRKSANPGYKPDPAKTFDPKGFP
                     TAAQGYALADFGPNLSNMAAKFQAQPEGLKWLSNWIGAPEKYHPKSLMPNLQLSAQDA
                     TDIASWLLSVPGEWPVKVDVPSVDDKEVTAALDDLIKLYVSKSGGFKKADGKTMAVSL
                     SEIDELVEKKLTTDDKLLYLGEKTISRLGCFGCHVIPGFENAKPIGTALNDWGIKSPA
                     RLDYGHIAEYLTDQPADETGTRDGTDLFYQEKVTHETRMGFLYQKLHRPRSYDYRKVS
                     EKYKTWDDRLRMPQFAWANDPAAVEEVMTFVLGLTNEKISGRYLPKTYARPAQTALAL
                     GRRDLNRFNCDGCHVLEMPKFTIPQGVKVAESFTDFKANLRSSYTSRGSDYLAELYPT
                     LTYDPQKKLDPNEIEKELGVTDDQGSAVTIEGMPIGLFENELTVQLWQP"
BASE COUNT         2289 a         3695 c         3813 g         2492 t
ORIGIN      
        1 ttgctcaaga gtgagtttgg gctcagtcag cggcagcttg gatatctggg gtcggcgttc
       61 atgctcgtgt atgccctgac atcaccgttt tcgggttaca cggtcgactt cttgccccgc
      121 cggctgttga tcacgctggg actcacattc tggagtttga tctgcgcggc cacggcgctt
      181 tcgcgcagtt tcttgcagtt gatttttttt cgcgccgccg agggcctggg agaatcgttt
      241 tacttcccgg cgtcgatgtc gattctggcc gattatcatg gcggccgcac gcggtcgcgg
      301 gcgctgggta ttcatcagac gagtgtgtat ctggggacgg ctggcggagc agtcctggcg
      361 ggttacttgg gagagcgtct gggctggcga tcgccgttct gggtgttggg tctggcgggg
      421 gttgcctacg cggtcttgct gggtttcgtc ttgatcgaac cgcggcgcga atccaccgat
      481 cgcgacgagg cacgacggca cgaggaggcc gacgttccgt ggaatcaatc accgccgagt
      541 tccgactcgt tggcgatgaa gctggggcga attctcacca atccggcggc ggccttgctt
      601 ttggcggttt tttgcggtgc gaatttcgtc gcggcgacgt tcctgacctg gctgccatcg
      661 tacatcttcg agcggttcga gctgggcatt ttgagctcat cactgacgtc gacgttctgg
      721 ccgctcgcga gtttaccggg agcgctcatg gggggtgtgg ccgccgatct gtcgtcgcgg
      781 cgggggcgag ggggccggat cagggtccag agcgtgggcc tgtgtgtcgc cgctccattt
      841 gtgttcttga cggggtgggc gacatcggtc cctgtcttga ttgccgcctt gattggcgcc
      901 ggcatctgca aggggattta tgattccaat atcttcgcct cgcttttcga tgtgatccga
      961 ccagaagacc gggggacggc ggctgggctg atgaacacgg tcggctggac aggcggcttt
     1021 gcggctccga cggtggtcgc catcgcgtcg gaacgacttg gcctgggagc aacgatcgcc
     1081 gcgaccgcgg ccgtgtatct gctggggggg atgctcgcgt acggcgcggc caggatggcg
     1141 gaaactcgac attttagaat ggcttgaagc ttgctcacca atttgagggg catcgcaaag
     1201 tgaattctgg gtcgctcagc aaatcatcca ccgaatccgg ctacgtctgg gctcgacggg
     1261 gcgacgtgaa tgccttcttc gggctgatgc tcgacaacat cggcggcatg atcttgatgg
     1321 caagcctcct ggttctgggt ttcggaatgc cgcggtcgtt cgtcttgacc cggatgattc
     1381 cggggacggc agttggcgtg ctggtgggcg acttgatcta tacgttcatg gcattccggt
     1441 tggcgcgtcg gaccggacga tccgacgtga cggccatgcc cttgggcctc gacactccga
     1501 gcacgtttgg tgctgtcttt ttgatcatcg gtccggctta ccaggccgct ctggggcgcg
     1561 gtcttgaacc cgatttagcg gcacgtcacg cttggttttt aggcatttcg atgctcgtgg
     1621 cgtcaggcat atttaaacta gcgtgtgccc cgtggagcgg ttggatcaga aaccgtgttc
     1681 cgcgtgccgg actgctcggt tcgttgacgg cgattgcact cgtactgatc agttttctgc
     1741 ctttgatgga cattgcgggt gagccggtcg ccgggttagt ggcgctgggc ttgatgctcg
     1801 cgaccctgac ggcgcggtgg cagtttcccg ggcgctttcc tggggctttg ggggcggtgg
     1861 tgatcgggtg cttgctctat tacggcatgc atctggtggg atggggcccg ggatctggga
     1921 gtgagggagc cgcgcctgca gtggcattgc gggcggttct gccggtgccg gtcgaagact
     1981 ggctggcctg gatcggcggg gcgtggagcg aggcggtcgg ttatctgccc gtggcgattc
     2041 cactcgcgct ggccacggtg gttgggggca tcgattgcac cgagagcgcc gcggcggccg
     2101 gcgacgacta tccgaccggc cagatcatcg cggctgaggg cttggcgacc ttggtggggg
     2161 gcattttcgg cggggtgatc cagtcgaccc cttatatcgg ccaccctgct tacaaggcga
     2221 tgggagcacg atcggcctat accctggcaa ccgcgatctt cgtcggtgcc gccgggatct
     2281 tcggctattt cgactggatc ttcttcatca tccccaaggc ggtgatcttc ccgatcctga
     2341 tcttcattgg cttggaaatc acgtcgcaat cgtttcacgc gacgcccgcc cgccattatc
     2401 cggcggtggc cttggcgtgc gtgccggcac tcgcatacct ggctttgctc acgctcaatc
     2461 agctcatgcc cgaactgggc aagccgtttg ctgagctcag gccccagacg cagcactgga
     2521 ttcaaacggt cacggtcgtg gccggcggcg gcggtttcat cgtcaccagc cttttgtggg
     2581 ggactgcgct ggcacacctg atcgacggcc gggtgcgccc gttggtggcg agcctgctgt
     2641 tggcggccgt cttttcctgg tttggcatca ttcattcgcc gctgccatcc agcccgatca
     2701 tgtcccccgg cgccgtaatc cgtcagctcg aagccgaagg acgcgccgcg gcgtcggcgc
     2761 ggcagacgcc ctatcactgg tcggccggat atgtcgcaat ggctctcgcc cttgtggccc
     2821 tctcccgctt tggcaccttg ccggccttca cggaattgga ggcacacaaa ccggtatcag
     2881 gctgagtttg gccagaccgg tgagcgtgtc gtcgtcatat ccgtcacaga ctgcaagtcc
     2941 gatatagacg ccacactgcc cgcgctatac cttattgtga agttgtgttt ttgcatcaaa
     3001 gtacggtaat tcgataccat gaattttatt ggcacgaaac ttgcgaccgg cggcggtgct
     3061 ttgggcggaa atcgccaaaa gaagtgctgg ccccgatttg cagcatttgg ccaagtgctc
     3121 cacccggttc tactattgag tttactggtt tccgctggcc ggaagtcgac cgcgtagtgg
     3181 aacgcctacg cgacagaaaa cttagttact ttggcggaat caagtgacgg ccggaatacc
     3241 gggagcagag cgcgtgttca gttcgatcgc cacgaaaccg tgagacgggc tcatcatgcg
     3301 taagggaatc ggtgtcagtc cgggcgtcgt ggtcggcgtg gcacatcgcg tcgagtcggc
     3361 gctgggcgcg atcgagcaag aaacccttga tcatgcgggt ttgatcgcgg ccgaggtcga
     3421 acggttcgcg ctggcggtgg ccgaatcggc gaccgagctc gaaacggtcg tccagaaggt
     3481 cgcagacgtg ctgggggctg ccgaggccga catcttcaag agccatctgc agatcgtcaa
     3541 cgatcccgcg ctcctgtcga aggtccgggc cttgatcgaa aatcagcagt tgacggcgtt
     3601 gtcggcgctc caggtcgtca tgaacggata cgtcgcacaa ctggcgcgga gccagcaaga
     3661 gttgtttcgc gagcggatga ccgacatccg cgacgtgatc ttgagaatcg agtcgcatct
     3721 gacgcgcaag aagatcgcgc atgtcgatcc ggcgggcgac tcgcgcaacg gtgacgagtc
     3781 ggtcattctg gtggcccatg agatcctgcc cagccaggcg atgagcctgg gggacctgcc
     3841 gatcgcggga atagttactg agctgggggg ctcgagcagt cattcggcga tcctggcgcg
     3901 aagtcgcggc atcccggcgg tctcgggcgt cgagggaatc atgagtgagg tcgagagcgg
     3961 cgacctgatg atcgtcgacg gccgcgacgg catggtgctc gtgcggcccg atcaagaagc
     4021 gacctccgcc tatcgcaagg ttcaacgcga gtttttcaat ttcaaggatc gcctgatcgc
     4081 caatcgcgat ctgccggcca aaagcgccga tgggacgcac gtcgagctgc ttgccaatat
     4141 caacaacctg cccgatgcgc aggccgccca caaggtcggc gccaccgggg ttgggctgtt
     4201 ccggaccgag tacctgttct tgacgcatca cgacgtgccg ggcgaggaag agcagtacga
     4261 gtattatcgc cagatcatct tgaattcgcc caaccagacc atcacgatcc gtacgctcga
     4321 tctagggggg gacaagacag taccttacct gggccgccgg ggcgagccca acccgttcat
     4381 gggctggcgc tcgacccgca tattcttcga gaatcccaag ttgttcgtca cgcagatccg
     4441 ggctatcctc cgggcgggtc gacacggcaa ggtctcgatg cttttcccca tgatcacgac
     4501 cctggaagag ctgctgttcg tcaacaacct tgtcaaagaa acgcgcaata acctccgccg
     4561 cgagggagtg gcattcggcg aggatgtaaa gaccggcgtc atggtggagg tgcccgcggc
     4621 cgccgtgtgc atcgacgcga tcctgcgcga gatcgacttc atttcgatcg ggtcgaacga
     4681 cctgattcag tacttggtcg cagcggatcg agacaatccc aaggtcgctc atctatgcga
     4741 ggctctctcg ccagccattt tcagggtgtt gcgcatggtt cttgatgcgt gccagcgcac
     4801 cggtacgccg gtgactctct gcggcgagat ggccggccag ccacgatcgg tcctggtcct
     4861 cttcggcatg ggcctgcgcc gattcagcat gagtcccgcg ttcatcccga ccgtcaagga
     4921 gttgctctcg tcggtgacca ccgcccagtc ggagcgcttt gcccatcacg tactgcagct
     4981 cagtaccagc gaggagatcc gctcgtacct gaccgcgcgg ttgcacgagg tgtcgagcac
     5041 actcgaggtt ttcgacagct cgtgacgagt gccaacgtgt tgctcggcgg cggccggcct
     5101 gctaggctat gccctcttcg atcgatgagc cgtctgggag tcgatcttgc acgcttaatc
     5161 gaggcatcgg atggaaacgc ggatatgacc aggctcccaa ttctcgaatg ctggaaatcg
     5221 gctggtgccg ttttcccgat aatgagaatg cgacgcggga cggcaattgc aacagattac
     5281 ggaagtctag gtcaagcaag tagttaatag cattgcaccg tgcggatttt gaaccggcat
     5341 cacctttgcg tgtggggcaa cggggtcgcg tatgcaacgg tgtcccgcga catcgaggcg
     5401 actgggaccg atgttattgg gaggctagag ccgagcgatg gcgaaacgtc ctagtgtcaa
     5461 ggaaattctg gaagccgccc gcaaaggcgg tcctgccaag ccggcggatg aatctgctcc
     5521 ggctgcggag gcagccgccg aggaaaccgc gcccgtggag gatgtcggtg atgtcgcggt
     5581 ggcggccgag ccagcgggtg caccggcgcc tgcggccaat gttccgaccg ccgcgtcgct
     5641 gggccggccg ctaaccctca aggaaaagct agccgcggct cgtgccgggg gagcaccggc
     5701 ggccgccgcc gccccagccc cggtaaagaa agcggccccg gctccggcgg ccgcgacagg
     5761 cggacctgcc gaggaagtca aggcggttcc tccgccggcg aagcctctgg gccggccgat
     5821 gacccttcag gaaaagctgg cggctgcccg cgggactgcc gcagcggccg gatctgcggc
     5881 tcccgcgtcc ggtgcgaagg cggctgcacc caagccggct gcgcccaagg ccgagcaggc
     5941 gcgtacactg ccgccgctgg gagagatcac cgaccccaaa gacctggccg aagcagctcg
     6001 acaagccgcc gccaagaaag ccaaagagat agccgccaag gcggccgcgg cggcgccccc
     6061 caaggcggcc gccaaaaagg gcccggccaa gccgcaaacc gtcccgcccc gaccgactcg
     6121 cgagcttgcc ggcgcaaccg ccgcggccga gccacggttc aatcgccgcg gctttttcct
     6181 gggcagcgtg ttcgtcacct gggcgatcgc ctggatcgtc gtggcctggg cgaccttcac
     6241 cgcggcactg tcggcaatga ccttgatgtc ggtccgcttc atgtttccca acgtgctcgc
     6301 cgagcccccc agcacaatca aggttggcct tcccacgaat tacgagcccg acgaggtcag
     6361 cgagcgattc aaggcggagt ggggcttctg gatcgtccgg tcgacgcgtt acaacggcca
     6421 ggatgtcatc tatgctctga agtcggtctg cacgcacttg ggctgccctc ccaactggct
     6481 gtcggccgag cagaaattca aatgcccatg ccacggcagc ggcttttaca tctcggggat
     6541 caatttcgag gggcccgcgc cccgtcccct ggagcgcttc aaggtcgcgc tggctgacga
     6601 cggccagatc caggtcgata agagtcaaat atttcagcaa gagttaggtc aatggtcgga
     6661 tccggatagc tttgttccgg cctgaccctt tccgagcgat aaatagactt cgcacccaga
     6721 catgctcccc gacggctcgt tcctcttcca ccggtgacct cgagagatgg cccatgccga
     6781 tcctggacaa gatcacagac aatcaacttt ggaagagcat cttccgccat cccatgccca
     6841 ccgatcggcg caaccgggtc gtggtcatgc tcaccaactt cttcctgcac ctgcaccccg
     6901 ttagcgtgcg gaaacaaggg atcgcccttt catacacctg gtgcatgggg ggaacaacgt
     6961 tcttcctctt cattgtcgag accgtcaccg gtgtactcct catgttttac taccggccca
     7021 cgctcgaaca cgcctataac gacatcctcg ccttgcgcga cgtgacgacg ctgggaatcc
     7081 tccgcgagct gcatcgctgg ggagcgcacg cgatggtgat cgcggtctgg ctgcacatgt
     7141 atcgtgtgtt tttgacagga agctacaaac cgccgcgcga gttcaactgg ggagtgggcg
     7201 tgatcctcct cgtgctgacc ttgctgttat cgttcacggg ctacctgctg ccgtgggacc
     7261 agctcgccat ctgggccatc acggtcggct cgaacatggc acgcgccacc cctgggttgg
     7321 gggttgaggg tccgggcgcc tcgttgctca agctcaacgg ggtgagcctg atcacgtcgg
     7381 gctccgatgc caagtttgcg ctcttggggg gacggagtgt cggcgagatg acgctgaatc
     7441 ggttttatgt gctgcattgc gtcgcgatcc ccctggccgc ggccttgttg atcatgatcc
     7501 acttctggcg ggtccgcaaa gatggcggca tcagcgggcc gatgtgatcg ggcgctctcg
     7561 ggcagcctcg cccttgccgc ggctctcggg cgcgcagccg cgagcggagg cttccggttg
     7621 ggcagggagc gaatcgctcg ggcgcgatga ctaagtagag tactgggctc ctcaagaaat
     7681 cacgaataca aaagacctat gcacaacgat ctgacagtag gcgtgatggc cgggctagca
     7741 tggtattact tgcttgcggc cgcgttgaac gcggctgccg cggcccgtat ttcctacggc
     7801 gagctcgtct ccgaaggggc ctcccgcatc ggcctggccc ccaagaccag acactttccg
     7861 gcctggctcc tggtcgcctt tctcggtctg tacagcgcgg cggtgctgat gatcctgggc
     7921 cggggcgtgt tgcccggcag cgttggggtg gcgtacggcc tgtgcgcgct ggccaacggt
     7981 ctcttggccg taatggcggg cgcggatgcc gcccatttcg ccgagatcaa ggcccacgga
     8041 catggcgagg gagattcgct cagtccgccc agcctggacg atcaccatcc tgccgtcggt
     8101 ctgggcaaac cgatcaaccg gacgctatgg accttgatct ggggctttgt ggccgtgctc
     8161 tttcaggcca tgggtgttgc atattatttg agcgcggcag cgcagactgg caacagcgac
     8221 cagactcccg tcaccggcat cttgatgcca cagttcatgc gcgacgcgat cgatttcgcc
     8281 gccgggccga cgacgttttt cgtgggcgcc acgatcggtt ttattgccat gatccggtgg
     8341 cggcggttca tggccaacgg cctggtcggc tgggcgatcg tcaacttgtt cttgctgtat
     8401 ttcggtctca gcatgaccga ttacgacttc cgggatattg tcaccaagcc cgacaacgtg
     8461 ccgatcgtcg gcctcttgat cctggtcggc tttttcttct ggtttggttt gcgccgcgcg
     8521 gtgatcaacg acgccggtcc gcccagggct tgcccacgct cgaggacctc gagcccgaca
     8581 agaccttgac gtggcccgac ctggtttaca ccgagctcat atgcatggtg ctcctgacga
     8641 tcttcctggt cgtctgggga attgcgctcc aggcgccgct cgagcagccg gccagctcga
     8701 cagttgcccc caacccgtcg aaggccccct ggtacttcct cgggctccag gaaatgcttg
     8761 tctatttcga tccctggatg gcgggcgtcg tcttgccgag catgatcatc gtcggcttga
     8821 tggcgatacc gtacattgat accaacaagt ccggcaacgg ctattacacc tacaacgatc
     8881 gcaagttcgc ttatctgacg ttccagtacg gattcgttat tctctgggtg gttctcattc
     8941 tactgggcac gtttttgcgt ggccccaatt ggaatttctt cggcccgtac gagttctggg
     9001 atctgcataa gctgatccct ttgaacaacg tcaacttgtc cgatatcgtg tggattcaac
     9061 ttttggggac ggccaagccg tcgaacatcc tggtccgcga gtttcccggg atcgccctca
     9121 ccttggcgta cttcacaatc gcgccctact tgctctaccg gttgttcttc aaaaaatacg
     9181 cagaacagtc ggggacgctc cgctatgtca cgctcgcggt tttgctcctg ttcatggcga
     9241 gtctgccaat caagatggtg ttgcggtgga cgatcaactt gaaatatgtg gtggcgattc
     9301 ccgagtactt tttcaatatc tgaggcgggt tcctcccgat ctcgttaggc ctttagcttc
     9361 cttggtgtac gtgattcgat ctcacaggac gaattgacca tgccggcttc cgaggaaacg
     9421 tatcgacgtc aaccgacact tcacctggtc ttcgcgattt cttcggtcgc gatgctctta
     9481 tccacggtct ggatggtgat ggccgaccat ttgcgcccat ggaaggaagt ccagcgcaag
     9541 ttccagtcgg tcgaacggga taaactcgaa gcggccgaga aggaaaagct cgaggagcag
     9601 aagcagaaat accagactca gctcgacgac atcgacgcca agatccaggc ggccgaggcc
     9661 gacgccgata cgcgcgcccg cgatctccgg gtgatcgaca acgagctcaa ggtgcttgag
     9721 ggacggaccg aaaagatcga caccgccaag cgatttcaga aggcagaact cgacagcaag
     9781 cgaagcctct acgacggcat gatcgaccgc ggtgaggagc cggcggcgcg gagatacttg
     9841 accacggtcg tgaaggatgc cgaagaccgg ctcaaatccc tctcggtcga tctggaaaaa
     9901 gcgcaggaga cgttgaagtc aaagaaggac acgaaggaaa agctgctggg ctttgtcgat
     9961 gacctcaaga aagagaaaga ccggcttacc cgcgaggccg atcgcgtcac gcgcgtgatc
    10021 gaacagaaag acgcccttta cggcggcacg ctccactggt acagccagcc gatggcgttt
    10081 ctgcgcggcc tgccgggcat cgacttgatg ccgccgacca agatccagca gatcagtctt
    10141 cccgagctga ccatcaatta taatttcaag gaggttccgc ggtatgaccg ctgcgcgacc
    10201 tgccaccagg ggattgaccg catcggttac gacaagaacg ctcacgggga agagatggaa
    10261 gaggtgttca ggtcgcatcc cttcctgacc accggtgcga ccacgctcga cccgcgcggc
    10321 aaggtcgtca cggcgggtct ttatctcgac gccaatggtc cccatccagt caacagcttt
    10381 ggctgcacga tctgccacgg cggccagggc tcgggcaccg acttcacgta cgcctcgcac
    10441 acgcctgact cgcgcgagga aggcaagaag tgggaggaag agtataactg gcacgagatc
    10501 catcactggg acttcccgat gctgcccagg cggttcatcg agtccggctg cgtgaagtgc
    10561 catacccagg tgaccgacat cccccaggcc aagaaactgc aagccggcta ccagcggatc
    10621 gtcaagttcg gctgcaccgg ctgccatacg atcggcggcg atgggtcgta tggtcctgat
    10681 ctcaccgacg agcggcaagt agggcccaac ctgtcgcaca tcgccgccaa ggacgcccag
    10741 gaatgggtca agaagtggat cgccaatccc cacgccttcc gtcccgattc acgcatgccg
    10801 cgattctacg gcttgaccaa caacggcgct accgaagatt ggcccaaaaa ctacgccgag
    10861 atccacgcga tcacccacta cctcttcgcc aagagcacgc ctcccgacaa cttcaccgat
    10921 cctccggcca agagcgaccc ggccaagggt aaagagctgt tcctccagaa gggctgcttg
    10981 gcctgccatc aacaccggcc gtaccaagtg gccgacatcc agctcggcga tcgcaagtcg
    11041 gccaatcccg gctataagcc ggatcccgcc aaaactttcg accccaaggg ttttccaacg
    11101 gccgcccaag ggtatgcact ggcggatttc ggaccgaatc tgtccaacat ggcggccaag
    11161 tttcaggcgc agcccgaggg cctcaaatgg ctgtccaact ggattggagc ccccgagaaa
    11221 tatcatccga aaagcttgat gcccaacctc cagctctcgg cgcaagatgc gaccgacatc
    11281 gccagctggc tactgtcggt ccccggcgag tggccggtca aagtcgatgt gccgagcgtc
    11341 gacgacaaag aagtcacggc agccttggac gaccttatca agctctacgt gtccaagagc
    11401 ggaggcttca agaaggccga cggcaagaca atggccgtct cactcagcga gatcgatgaa
    11461 ttggtcgaaa agaagctgac cacggatgac aagctcctct atctcggcga gaagacgatc
    11521 tcgcggctgg gctgcttcgg ctgtcacgtc atcccgggct tcgagaacgc caagccgatc
    11581 ggcacagcgc tcaacgactg gggaatcaag agtcctgccc gtctcgatta cggccacatc
    11641 gccgaatatc tgaccgatca accggccgac gaaaccggaa cccgcgacgg cacggatctc
    11701 ttctatcagg aaaaggtcac gcatgagacc cggatgggct tcctctatca aaagctccac
    11761 cgcccgcgca gctatgacta tcgcaaggtg agcgagaagt acaaaacgtg ggacgatcgg
    11821 ctgcggatgc cccagttcgc ctgggcgaac gaccccgccg cggtcgagga ggtgatgacc
    11881 ttcgtcctgg gcttgaccaa tgagaagatt tccgggcgct atcttcccaa gacgtacgcc
    11941 agaccggcgc agacggcgct ggcgctgggt agaagagact tgaatcggtt caactgcgac
    12001 ggctgccacg tcctcgagat gcccaagttc acgattccgc agggggtcaa agtcgccgaa
    12061 tccttcaccg atttcaaagc caacctgcga tcttcatata catcgcgcgg cagcgattac
    12121 ctggctgagc tttaccccac cctcacgtac gatccgcaga agaagctcga tcccaatgag
    12181 atcgaaaaag agctcggagt caccgacgat caggggtcgg ccgtcaccat cgaggggatg
    12241 cctatcggcc tctttgaaaa tgagctgacg gtgcagctct ggcagcctg
//
LOCUS       DATAQL010000531         4813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2648748, whole genome shotgun sequence.
ACCESSION   DATAQL010000531 DATAQL010000000
VERSION     DATAQL010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648748"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..925
                     /locus_tag="VHS97_09115"
     CDS             98..925
                     /locus_tag="VHS97_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448401.1"
                     /translation="MPISRDTNLLDSGTYQLGTRASAPTDLQALRDGPYSLHHILPYR
                     YPLFVGFLAETYATKYGGNTPNWPGGSTDQIAKQTAGILNVVSRFGSEQHTIPSKFAW
                     MGPNLFQGPSGNLRVDDPGSNPEERKPISYRPAVWDALMKVADKVNGLVSTLGSNNQG
                     YTVTTTKPLTVGDLEDLLNLLSPIADVGHGNALSFTDTDWIVMSTSDPGFSKDFLPNA
                     MVSVAVKQRFSALPRNKIQNFRPSYSGATTDLTPRLWRLRQSTEPIPTDVIKGPRLQ"
     gene            1505..3475
                     /locus_tag="VHS97_09120"
     CDS             1505..3475
                     /locus_tag="VHS97_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX3448402.1"
                     /translation="MGEPLALDLNSGRSMVRHLRRRSGFPAVFWLFSAHLLAGPECRT
                     HAEGVTGNIVFAGDSLTAGIGAAPQMSYPAQCLTILDRGWTGHNSGVSGQTGRQIIED
                     YDAQIGRHCDRSKPLNVLVLWEATDDLASGAPPEEVAENLARICARARATGFDRVILL
                     TSTPRSNFPGTSAIPGTPEQQRRTYNRRRDAVDARLMALYPTVDAVVPLHSDGQIGVD
                     GAELNPVYFAGDRVHMNDRGYFVVAALVAERIRTFGPRRIIPGRSWPSPRGHHHLVLQ
                     GDGNLVLYGPQRRALWASGTEGQPAAEAVMQPDGNLFVNGTRRGNRVGTGKALWASGT
                     NGLVGADLRVQDDGNVVIYHKGRAAWSTGTGTWTRPRNYSPPAPPTTWKEHWGGPTGI
                     HDQLVKLSRYNDDVAVYFDRDMPREDAEWLLPITTRIWQYTKKTYGDFGGPDDRLFAI
                     FHKGRYGGGHPSTYFDSHHDDRNVTDIGLDAWDPSKVGYIIHEVGHIVEGASNGVLGS
                     PAYGHGIWGDSKWAEFFEYDLRVGIGQVKEAEDALRRFTAAVDNFPRPKTRWFRDFFH
                     PLWRDHGHAQVMVNYFRLISKHYPKETLEDGKGLKFSRGKMNWGEYIHFMSGAAGKDL
                     RPLATRAFGWPAGWESQFQKARAEFPGVLYPE"
     gene            3936..4079
                     /locus_tag="VHS97_09125"
     CDS             3936..4079
                     /locus_tag="VHS97_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448403.1"
                     /translation="MTLPFILTLRGEDVLQVVLFAGASEVLALLGHGDEADAHLVGAR
                     PIC"
     gene            4358..>4813
                     /locus_tag="VHS97_09130"
     CDS             4358..>4813
                     /locus_tag="VHS97_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448404.1"
                     /translation="MAGSAQGEHIFNAVGDLVMELASYLQTVLISRNLLLNPPGNIFT
                     FNTTTNAPYVAAQQTLNSLRQLAQDAIDARDNAEFRTADRGDPANSSRYSTFASITRT
                     AVRQLATGAASELTQGYTQLNRFLDNIDYYTIDQTARTERRRELPATRRD"
BASE COUNT          885 a         1554 c         1509 g          865 t
ORIGIN      
        1 tcggaaatag ggatgtcccg tttaccgctg cttttaccgc tatcccgatt cctttgctcc
       61 agtctccggt gttcgtccgt gattacttgg aggtccaatg cctatttcac gagatacaaa
      121 tctgctcgac agtgggacat accaactggg cacaagggcg tcggccccga ccgatcttca
      181 agccctgcgt gacggcccat actcgcttca ccacatacta ccttatcggt atcccctctt
      241 tgttggcttc ctcgcggaga cctacgccac caagtacggt ggtaacactc cgaactggcc
      301 tggtgggtcc acggatcaga tcgcgaagca gacggctggg atcctcaatg tggtgagccg
      361 tttcgggagt gaacagcaca cgatccccag caagttcgcc tggatggggc cgaacctctt
      421 tcaaggtccg agcggcaacc tccgggttga tgatcccgga agcaatcctg aggagcgaaa
      481 acccatctcc tatcgaccgg ccgtatggga tgcgctcatg aaggtagcag ataaggtcaa
      541 cggtcttgtt tccaccctgg gcagcaacaa tcaggggtac accgttacga caacaaagcc
      601 cctcaccgtc ggcgatctcg aagacttgct taatctattg agccccatcg ctgatgtggg
      661 acacggcaac gccctctctt ttacggatac tgattggatc gtgatgtcca cgagcgatcc
      721 gggtttcagc aaagacttcc tgccgaacgc aatggtgtcg gtcgccgtga aacagcggtt
      781 cagcgcgctc ccgcggaaca agatccaaaa cttccggccc tcgtactccg gggcgaccac
      841 agatctgact cctcgcctgt ggcggttgcg gcagtcaaca gagccgatac cgaccgacgt
      901 gattaaaggt ccgcggctac agtgaaaggc gctcctggcc ggatggcatg gccgcgtcat
      961 tgagacgcga caggcacctg atcgccatcc ccgcggtcat cgccgcaacg atgccggcaa
     1021 gggcgagggt acgaagcacg tccgcggcaa cgactcgcgg cctcggccta tcgccgatcg
     1081 tccccgtcgt cgggccggct gcgccggccg agtcagagcc cgcccagttt cctcagccca
     1141 acggcccgcg tccgatccct tgacgttcca ggggtcatcc ggtccctggg cgtcgcgttc
     1201 gcagtcggcc ggcgatcgcg aactcactcc gctttcggcg ccagcgtagc cgcgggtgac
     1261 ggagtgtcag aatgacctga aaagacaggt ctcttggccg tctccgacgg cagtatgggc
     1321 tctttgagag gagcatcggc ttcgcctttc aacgagaagg cgaaccggtc tgcaccgccg
     1381 tggcggaggg aggaccaagg gccaatggcc cagtttgtca cgaatcgttg ccatacgcct
     1441 ttatgggtgt gacgggccgg tcaggtcgct gagggcccgc tagcccgagc atcgaggggc
     1501 cgagatgggc gagccactcg ccctggactt gaactccggg aggagcatgg tgagacatct
     1561 tcgcaggcgg tccggtttcc cggcggtgtt ttggttattc tccgcgcacc tcctggcggg
     1621 gccggagtgc cggacgcacg ccgaaggagt gacggggaac atcgtgtttg ccggcgactc
     1681 gctcacggcc gggattggcg ccgctccgca gatgtcgtac cccgcccaat gcctgacaat
     1741 cctcgaccga ggctggacgg ggcataattc gggagtctcg ggacagacgg gacggcagat
     1801 catcgaggac tacgacgccc agatcggccg ccattgcgac cggtccaagc ctttgaacgt
     1861 ccttgtgctc tgggaggcga ccgacgacct tgcttcgggc gccccccccg aggaagtggc
     1921 ggagaacctg gcccggatct gtgcccgggc gcgggccacg ggcttcgacc gggtcatcct
     1981 cctgacctcg acaccccggt cgaatttccc cggtacgtcc gcgatccctg gcaccccgga
     2041 acagcagcgg cggacctaca accggcggcg ggatgccgtc gacgcgcggt tgatggcgct
     2101 ctacccgacg gtcgatgccg tcgtgccgct ccactccgat ggccagatcg gggtcgacgg
     2161 ggccgagctc aacccggtct acttcgccgg cgaccgggtc cacatgaacg accggggcta
     2221 cttcgtcgtc gcggccctcg tcgccgagcg gatccggact ttcggccccc gccggatcat
     2281 cccgggccgg tcctggccgt cgccccgagg gcatcatcac ctcgtcctgc aaggcgacgg
     2341 caatctcgtc ctctacgggc cgcagcgccg ggcactctgg gcgtcgggta ccgaggggca
     2401 acccgcggcc gaggcggtga tgcaacccga cggcaatcta ttcgtcaacg ggacccgccg
     2461 gggcaaccgg gtcgggacgg gcaaggcgct ctgggcgtcc ggcacgaatg gcctcgtcgg
     2521 tgccgatctg cgggtccagg atgacggcaa cgtcgtgatc taccacaagg gccgcgccgc
     2581 ctggtctacc ggcacgggga cctggacccg gccgcgaaat tactcgcccc cggcgccgcc
     2641 gaccacctgg aaagagcatt ggggcggacc gacgggcata cacgaccaac tggtcaagct
     2701 ctcgagatac aacgacgacg tggcagtcta cttcgaccgg gatatgcccc gggaggatgc
     2761 cgaatggctc ctcccgatca cgacccggat ctggcagtac acgaagaaga cgtatggaga
     2821 cttcgggggc ccggacgatc gcctcttcgc gatctttcac aaggggagat acggcggcgg
     2881 gcatccctcg acttacttcg actcgcacca cgacgatcgc aacgtgaccg acatcgggct
     2941 cgatgcctgg gacccttcga aggtcggcta catcattcac gaagtcgggc acatcgtcga
     3001 aggcgccagc aacggcgtcc tgggctctcc ggcgtacggt cacggtatct ggggcgacag
     3061 caagtgggcc gaattcttcg aatacgacct ccgcgtcggg atcgggcagg tcaaggaggc
     3121 cgaggatgcc cttcgacgat tcaccgccgc cgtcgacaat ttccctaggc cgaagacccg
     3181 atggttccgc gacttcttcc acccgctctg gcgcgatcac ggccatgccc aggtgatggt
     3241 caattatttc cgcctgatct caaagcatta tccgaaggag acgctcgagg acggcaaggg
     3301 cctgaagttc tcccgcggga agatgaactg gggtgagtac atccacttca tgagcggggc
     3361 cgccgggaag gacctcaggc cgctcgcgac ccgggccttc ggctggccgg ccggatggga
     3421 atcccaattc cagaaggctc gcgcagagtt cccgggggtg ctctaccccg aatagccccc
     3481 ggcaatccgg acatatgtcg tgccgctgga caacatatgg tccggatcag ccttctgaaa
     3541 catatgtccg gcccggcttt ttggagtgcg aaggagcggt cggacatatg tctagaaggt
     3601 aggggctaac ccatgatcgt cgagacgggg tagggcaacg agtcgtgatg cactcttctc
     3661 tccatgatcg tttcccagtg cggtgagttg accgttcgtc agccggcccg aaggtgtccc
     3721 acgttcccat tcggcccagc ccgatcgccg agccggccgg cccgtcgggc tgagtcgcac
     3781 gtctcggctc tccctggccc gaagccgtct tcggcgcgtc cacggatcag ccgccgcgcg
     3841 aaggtgcgac ttccgttcgg ggtagcggac gtccttgtgg atggttcact cgccctggcc
     3901 ttggcctccg cggcgcgctc cttggcggtc cgctgatgac gctcccgttc atccttacgc
     3961 tgcgcggcga ggatgtcctg caggtcgtcc ttttcgccgg tgcgagtgag gtcctggccc
     4021 tacttggtca cggcgatgaa gccgacgcac atctcgtcgg tgctcgcccg atctgctgac
     4081 ggggccgggc aggatcgtcg gtcccggggg ccgtcgcggc ggagaaatcg gggtggatcg
     4141 cccccggcct gtccacgacc ggtctcggct cgcattcctc tcagtagggc cgttgcgcgg
     4201 cacccgtggg tgaagcccac aagggacggc tcggttccag ctcgagagga ggggacctcg
     4261 cccggtcgac cgcgatcggg ggggggtcgc ggcgcgaccg gaccgaaaac ggtgacgaat
     4321 ccttggaaac cctgaacgaa cgggaggtca taccaaaatg gccggaagtg ctcaaggtga
     4381 acacatcttc aacgcagtcg gagatctcgt catggagctc gctagttact tgcaaacggt
     4441 cctcatttct aggaacttgc tcttgaaccc ccctggaaac atcttcacct tcaataccac
     4501 cacgaacgcg ccgtatgtcg cggcccaaca gaccttgaac agcctccgcc agctcgcgca
     4561 agatgcgatt gatgcgaggg acaatgcgga attccggacc gcggatcggg gggaccccgc
     4621 gaattcgtcc cggtactcga cttttgccag cataacaagg actgcggtcc gtcagctcgc
     4681 cacaggggct gcgtcggagc taacccaggg ctacacccag ctcaatcggt ttctggacaa
     4741 tatcgattat tacaccatcg atcaaacggc gcgcacggag agacgtaggg aattgccggc
     4801 gacgcgacgg gac
//
LOCUS       DATAQL010000532         9690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1427045, whole genome shotgun sequence.
ACCESSION   DATAQL010000532 DATAQL010000000
VERSION     DATAQL010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427045"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="VHS97_09135"
     CDS             <1..1880
                     /locus_tag="VHS97_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448405.1"
                     /translation="RLFRQLAEWRFGSKNKAGNGIGLASDDVAVKNQRPVRTGRRPDG
                     LRPDGIPAILFVVKLMPKSRILACAFLIVAGVSATSSVRGLSVSADSRECLVIRLVHP
                     DRQAARVSMLFEGARSPHPAAALAAWKRATRDPNQLGKPLEAVISCFNPAMTPEWLVM
                     DGAQIGFDLSAADGRPRWFAIVPRDDGTLSAAITAQRLTEGSRDLPLTVEGRRIDVER
                     LGRAGAVVAARSGETLILGTSREELMRGLRRIGSGMMPAVPKDSEFVTPGAVAPMAPQ
                     FPFDSGLIFDLEPGLISAAVGSITYRRAVAFLQGLGCRRIHGNLGLTGDVVAIEVTTM
                     LQRGALASPALSKPSVAVDPAWLEWIPAAGAMAVLSVALGPGEPFWESAFALADRVDR
                     ADPARAALAPLRARFNLVAAAAGARPESDLWPHLRGVTAGVMGDPDRPGRPNGVVVVL
                     HADADANAHRLATDVLPRLASLLAAQKQGEVPRQGFRPRPVGDTQLIAKLGGRPLTIS
                     RRSRDVVISWGDEMQKALEDTVAHPDRSVALLCNGWIRAGKMPPQRVGAVWPARCWPV
                     FRGLDPTTPAWQTLAQGPPTVWWGWNDLNTAHDSIFAGDLRLRVRGFLEELPLDPSLL
                     K"
     gene            2331..2621
                     /gene="groES"
                     /locus_tag="VHS97_09140"
     CDS             2331..2621
                     /gene="groES"
                     /locus_tag="VHS97_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX3448406.1"
                     /translation="MALKPLGDRVVVEREEAKDTTAGGIVLPDTAKDKPQKGKVVAVG
                     DGRITKDGKRRELQVKVGEQVLFTSYAGEEFKIEGDKKVLLMREEDILAVIG"
     gene            2665..4296
                     /gene="groL"
                     /locus_tag="VHS97_09145"
     CDS             2665..4296
                     /gene="groL"
                     /locus_tag="VHS97_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244618.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX3448407.1"
                     /translation="MAAKMIEFDQDARQSLQRGVAKLARAVKVTLGPRGRNVIIQKSF
                     GSPTVTKDGVTVAKEIELEDKYEDMGAKMVKEVASKTSDVAGDGTTTATVMAEAIYNE
                     GLKAVVAGVNPMLMKRGMDKAVADIVEQLQKLSTKVTTKTETEQVATVASNFDSEIGK
                     MIAEATEKVGKDGVITVEEGKTLKTEVEWVEGMQFDRGYLSPYFVTNPTSMETVLEDA
                     YILIHEKKISVVKDLVPVLERVAQTGKPLLIIAEDIEGEALATLVINKLRGTFRSAAV
                     KAPGYGDRRKAMLEDIAVLTGGKPIFEALGIELENVSLEDLGRAKKVIIDKDNTTIIE
                     GAGSHDAIKGRIESIRREIADTKSDYDREKLEERLAKLAGGVAKINVGAATESEMKEK
                     KARVEDALHATRAALEEGILPGGGVALLRASKSVKTKGLSHDEEIGFNIIVKACASPL
                     YQIAENAGQDGGVVVSKVADGKGNFGYDALKDEYTDLVKAGIIDPTKVTRYALQNAAS
                     VSTLLLTSDALIADAPKDDEKKPGGGGHGGYDDMY"
     gene            4461..4919
                     /locus_tag="VHS97_09150"
     CDS             4461..4919
                     /locus_tag="VHS97_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448408.1"
                     /translation="MARRNTDGSHKFTRVGKLLGREEEGDKAVIVQGGEHTRVAAIGP
                     GPDHNALPDTEELVDSGDVFDIRHVHNLTTGRRRVVVLPPDLCRRLDITEGTPLRVVE
                     HEGRFEVIPMKLVPASDDRSSALAGLLREVTAENLHSEVDTGYAVGRESW"
     gene            4919..5254
                     /gene="mazF"
                     /locus_tag="VHS97_09155"
     CDS             4919..5254
                     /gene="mazF"
                     /locus_tag="VHS97_09155"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: protein motif:HMM:NF007386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HEX3448409.1"
                     /translation="MVASFVPQRGDVVWIDLNPQAGHEQAGRRPAVVLSPVAYNRKVG
                     LALFCPVTSQIKGYPFEVLIPAGLAVKGAVLADQVKSLDWRVRKAELISEMPGAVINA
                     VLAKVRTLL"
     gene            complement(5341..6138)
                     /locus_tag="VHS97_09160"
     CDS             complement(5341..6138)
                     /locus_tag="VHS97_09160"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX3448410.1"
                     /translation="MDSPPSSTPSETARSYPATVVKTPNPFSPRFLFSSLRNQLISGL
                     IFSLPIVITFWIIYWIFLTLERFLLNPVAALIHRIIAWMRNYPFFAELELPDWWYNVA
                     SPVLAIVLMIVILYMLGLFLRSWVYRTLDWFLLHVPIVATIYRAVRNVVNSLGSQFRG
                     GADFKRVVLVPFPHPGMRSLALVTNSLRDVTTGRTILTVCVLTGVMPPAGFTLFVPEE
                     SVTSIAWSVNETLQAILSGGITAPPTIHFSEGVAAPPHSVAGAVVDV"
     gene            6408..7394
                     /locus_tag="VHS97_09165"
     CDS             6408..7394
                     /locus_tag="VHS97_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3448411.1"
                     /translation="MTARQAAPEPTTAKTALVGGVTLHLSVPDATDQNWIGQDAILRQ
                     LLACWLVVDPRDLPLSPRLIGPPGIGKTTLAMAAAHLRKQPLYINQCTADTRPEDLII
                     TPVLAESGKIAYHASPLLSAMLAGGVCVLDEGNRMNEKSWASLAPLLDHRRYVESIVA
                     GITIPAHPDFRVCVTMNEDESTYEVPDYILSRLQPTLTLGFPTRDDEMSILKYHLPFA
                     EDALLALTVEFLQQAHRLKLDFSPRDGINLLRFAIKRMAQDPAHPLGRDVAWREALFC
                     VLGEEAADLQNLARRKRSAMGGQHAPMGLGDLFFGPEDPLHPDAEFEDEDDD"
     gene            complement(7461..9128)
                     /locus_tag="VHS97_09170"
     CDS             complement(7461..9128)
                     /locus_tag="VHS97_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3448412.1"
                     /translation="MTNEQTEIPPATIIDERFAVPTRVLGEEPACRTSLESERFGLLA
                     APKKEASDFDRRMENWIASNLDLEEAAGSTPHSPWIRLIDGLPGLKRRHQQKQLEKGA
                     ETALLGWARFHVVYKGRITVVRLSDKALVRESQIRELACDLIDLIEAGNHRVVLNFSV
                     VERLASWVVLVVGEAQRRCESADGGALKICGLPQQLASIFPTMGFNLRNALHVDEASA
                     IESPWPASSSPRTLPIEILSAITSVAGLPPIRGGVPSDAAEFSTPSAPKIPASFTVPR
                     ARAESRIKDELVLDVQVGSSKSRSVVVTSNTFVIGRDRECDLRLGSVMVSKRHAVIER
                     RDHCILLHDLGSTNGTVLNGRLLRNQERVVKHGDRIQIGPIIATLSVGPRRAETTRVD
                     EMVSGWLQSEGSFAKSCEHDSRATEPFPACDEAVSEPDHRIKDEVIQGVLVITPQVSD
                     LVDDEAIELFRSRLHGLYEQNAPRHVVVNLECVRHLNAKVIGMLLAHHLRLDRSGGAI
                     RICQAHARLMAVLHGVRLTEIVECQPTLDEAVLAAWPGTTSRLSADD"
BASE COUNT         1948 a         2839 c         3037 g         1866 t
ORIGIN      
        1 gtcggctctt tcgccagctc gccgaatggc gcttcggttc caagaacaaa gccggtaatg
       61 gaatcggtct tgccagtgac gacgttgcgg ttaaaaatca gcggcccgtg cgaactggca
      121 gaagaccgga cggactgcgg cccgacggta ttcccgcgat tttgtttgtg gtgaagttga
      181 tgccgaaatc caggattctc gcgtgcgcgt tcttgatcgt tgccggagtc agtgcaactt
      241 caagcgttcg aggtctaagt gtttctgctg attctcgcga gtgcctggta atccgcctgg
      301 ttcatccgga tcggcaagcg gcgcgtgttt ccatgctgtt tgagggtgct cggtctcctc
      361 atccggcggc ggcgttggct gcctggaaac gcgcgacgcg cgaccccaat caactgggca
      421 agccgctcga agcggtgatc tcgtgcttca atcccgcgat gacaccggag tggctggtga
      481 tggacggcgc ccaaattggc ttcgacttaa gcgcggccga tggcaggccg cgctggttcg
      541 cgatcgtgcc gcgcgacgat gggacgctct cggccgccat tacggcccag cgactgaccg
      601 agggcagtcg cgatttgccg ctgaccgtgg agggcaggcg gatcgatgtc gagcggctcg
      661 ggcgcgccgg ggcggtcgtc gcggcgcggt ctggcgaaac cttgatcctc ggtacctcgc
      721 gcgaagaact catgcgcggc ttgcgccgaa tcggttccgg aatgatgccg gctgtaccaa
      781 aggactccga gttcgttact ccgggggcgg tggccccaat ggcgccccaa ttcccgttcg
      841 attcgggcct gattttcgat ctggagccgg gcctgatttc agccgctgtc ggatcgatta
      901 cgtatcgccg cgccgtcgcg ttcttgcagg ggctcggatg ccggaggatc catggaaatc
      961 tgggcctcac tggggatgtt gtggcaatcg aggtgacgac gatgctacaa cgcggcgcgc
     1021 tcgcctcgcc ggcactgtcg aagccgtcgg tcgccgtgga tccggcgtgg ctcgaatgga
     1081 tacccgcggc gggtgccatg gctgtgctct cggtggcact cgggcccggt gaaccgttct
     1141 gggagtcggc gttcgcgctc gccgatcgcg tcgaccgcgc cgacccggcg cgcgcagcgc
     1201 tggcaccgct gcgggcgaga ttcaatttgg tggctgccgc ggctggtgcc cggcccgagt
     1261 ccgatttgtg gccccacttg aggggcgtca cggccggcgt gatgggcgac ccggaccgac
     1321 ctgggcggcc gaacggcgtc gtcgtcgtcc tgcatgccga cgccgatgcc aacgcccatc
     1381 gcctcgcgac cgatgtcctg ccccgtttgg catcgctatt ggccgcgcaa aagcagggag
     1441 aggtgccacg ccaagggttc aggccgagac ctgtcggcga tactcaactc atcgcaaaac
     1501 ttggcgggcg cccgctcacg atctccagac gcagtcggga tgttgtgatt tcctggggcg
     1561 atgagatgca gaaagcacta gaagatactg tggctcatcc cgaccgctcg gtcgccttgc
     1621 tctgcaatgg ctggatacgc gccggcaaga tgccacctca gcgcgtcggt gcggtctggc
     1681 cggctcgatg ttggccggtt tttcggggac tggatcccac tactcctgcc tggcagacgc
     1741 tggctcaggg tccgccgacg gtctggtggg gctggaatga cttgaatacc gctcacgatt
     1801 ccatcttcgc gggcgacctc cgtctccgcg ttcgcggctt tctcgaagag ctcccactcg
     1861 acccgtcgct gctcaagtga caaggggcag ttccgaagac tcgacacgac cttcagggtc
     1921 gtataataac cgtcagccat ctgtagatac catcatcacc tcatccggcg ggccaggata
     1981 catacgggtt gcgccatgaa gcgccagcgc cctggggtcg aagaaacatc ccttcatctt
     2041 cccagctcaa tccgccgggt cggctgccga attggcagtc gaccaagaca atgcgtcgag
     2101 atcggcagga agtccccagc gagtcgctca attccgctcg acgtaactgt atttctaata
     2161 attggttgca actaaattgc gacaaggcac gttccttgca taatgtgcaa ggcaaaagca
     2221 gcgtgtttgg cggtgcgccg accacggggt tgttgatttc tgagcatcgc attgtcggtc
     2281 ccgggcattc agcctttcgt aagggtcgag cgagatcaag gagcggttcg atggcgttga
     2341 aaccactggg cgatcgggtt gttgtggagc gtgaagaggc aaaggacacc accgccggcg
     2401 ggatcgtcct gccggatacg gccaaagaca aacctcagaa ggggaaggtc gtggctgtcg
     2461 gcgacggtcg gatcaccaag gatggcaagc gccgcgagct tcaggtcaag gtcggtgagc
     2521 aggttctatt cacctcgtac gctggcgagg aattcaagat cgagggcgac aaaaaggtgt
     2581 tgctgatgcg cgaggaagac atcctggccg tcatcggcta atgagtcaag acatccatct
     2641 ctatttatcg ggagtgaatc acgcatggca gccaagatga tcgaatttga tcaggacgcc
     2701 cgtcagtcgc ttcagcgcgg cgtggccaag ctggcccgcg cggtcaaggt gaccctgggc
     2761 ccgcgcggcc gcaacgtcat catccagaaa tcgttcggct cgcccaccgt caccaaagac
     2821 ggggtcacgg tggccaagga gatcgaactc gaagacaagt atgaagacat gggcgccaag
     2881 atggtcaagg aagtcgcgag caagacttcc gacgttgccg gagacggcac gaccacggcg
     2941 acggtgatgg ccgaggcgat ttataatgag ggtttgaagg cggtcgtggc cggcgtcaac
     3001 ccgatgttga tgaaacgggg gatggacaag gccgtcgctg acatcgttga gcagctccag
     3061 aagctcagca cgaaggtgac gacgaagacc gagaccgagc aagtcgcgac ggttgcctcc
     3121 aactttgatt ccgagatcgg caagatgatc gccgaggcca ccgagaaggt cggcaaggac
     3181 ggcgtgatca cggtcgagga aggcaagacc ctgaagaccg aggtcgagtg ggtcgaaggg
     3241 atgcagtttg accgcggcta cctgagccct tacttcgtca ccaatcccac cagcatggag
     3301 accgtcctcg aagacgccta catcttgatc cacgagaaga agatctcggt ggtcaaggac
     3361 ttggttccgg tgctcgagcg cgtggcccag acgggcaagc ctctcttgat cattgccgaa
     3421 gacatcgaag gggaggcgct ggcgaccctg gtcatcaaca agttacgggg cacgttccgt
     3481 agcgctgcgg tcaaggcgcc cggttatggt gaccgccgca aggccatgct cgaggatatc
     3541 gcggtcttga ccggcggcaa gccaatcttc gaggcgctgg ggatcgagtt ggaaaacgtc
     3601 tcgctggaag accttggtcg cgccaagaag gtgatcatcg acaaggacaa tacgacgatc
     3661 atcgaagggg ccggcagtca cgacgcgatc aaggggcgaa tcgagtcgat ccgccgcgag
     3721 atcgccgaca ccaagagcga ctacgaccgc gaaaaactgg aagagcggct cgcgaaactg
     3781 gccggcggcg tggccaagat caatgttggc gccgccaccg agagcgaaat gaaggaaaag
     3841 aaggctcggg tggaagacgc gctgcatgcg acccgcgcgg ctctcgaaga aggaattctg
     3901 cctggcggcg gcgtcgcgct cttgcgggcc tccaagtccg tcaagaccaa gggcctttct
     3961 cacgatgaag agatcggctt caacatcatc gtcaaggcct gtgcatctcc cctctaccag
     4021 atcgccgaaa acgctggcca ggatggtgga gtcgtcgtgt ccaaggtcgc cgacggcaag
     4081 ggcaatttcg gctacgacgc cctcaaggac gagtacaccg acctggtcaa ggcgggcatc
     4141 attgacccga caaaagtaac tcgttatgcc ttgcagaacg ccgccagcgt gagcacgctg
     4201 cttctgactt ccgacgccct gatcgccgac gcccccaagg atgacgagaa gaagcccggc
     4261 ggcggcggcc acggcggtta tgacgatatg tattgagcaa tcgagcgaag ttcagcgaga
     4321 ggctgttgat ttcttgacga gggggcgccg ggagtttccc gacgtccctt tcttattgac
     4381 ggatccggca cggttcgggt agtctgtcag tcagtaatcc tgaatcgctg atttcctgaa
     4441 caagagattt ggacccttca atggcacgac gtaacacaga tgggtcacat aagttcacac
     4501 gcgtcggcaa gttgctcgga cgcgaggagg agggggacaa ggcggtcatc gttcaaggcg
     4561 gagagcacac gcgcgtggca gcgattggcc ccggtcccga tcacaacgcg ctcccggata
     4621 ctgaagagct ggttgattcg ggtgacgttt ttgacattcg tcacgttcat aacctaacga
     4681 ccggcagacg gagagtcgta gtccttccgc cggacctgtg tcggcggctt gacatcaccg
     4741 aaggtacgcc tctcagagtc gtcgagcacg agggtcgatt cgaggtaata ccgatgaagc
     4801 ttgttccggc atcggacgat cgttcgtcgg cactcgccgg cttgcttagg gaggtgaccg
     4861 ccgaaaactt gcactcagag gtcgacacgg gctacgctgt cggccgggag tcttggtaat
     4921 ggtcgcgtcc ttcgtccccc agcgtgggga tgtcgtttgg atcgatctga atccgcaagc
     4981 cggtcacgag caagcaggcc ggcgccccgc ggtggtgtta tcgccagtcg catacaatcg
     5041 caaggttggc cttgctctgt tttgcccggt caccagtcag atcaagggat acccattcga
     5101 ggtgttgatc cccgcaggct tggcagtcaa gggcgctgtg ctcgctgatc aggtcaaaag
     5161 tctcgactgg agggtccgta aggcggaact gatttctgag atgccgggcg ctgtaatcaa
     5221 tgcggtgcta gcgaaggtgc ggactctcct ctaggtgcgg ctgccgcgcc ggcttggcgc
     5281 gagtcgcagc gattgagaaa tgtcggggac gcattcctgg gtcgagcgct gccgatgtcg
     5341 ctaaacgtcg accacggctc cggcgacgct gtgcggtggc gcggccacgc cttcagaaaa
     5401 atgaatcgtc ggcggtgccg tgattcctcc ggacaggatc gcttgcagag tctcgttaac
     5461 gctccaggca atgctcgtca ccgactcttc cggcacgaac agggtgaagc ccgccggcgg
     5521 catcaccccg gtcagtacgc acaccgtcag gattgttcgg ccggtcgtca catcgcgcag
     5581 agagttcgtc accaatgcga gtgatcgcat tcctggatgg ggaaaaggca ccaggaccac
     5641 ccgcttgaaa tcggcgcccc cgcggaactg gctccccagt gaattgacta cgttgcggac
     5701 cgccctgtaa attgtggcga cgatcggcac atgcagtaga aaccagtcga gcgtgcgata
     5761 cacccacgaa cgtaggaata atcccaacat ataaagtatc acaatcataa gcacaattgc
     5821 caagacaggc gaggcgacgt tgtaccacca gtcgggaagc tcgagttcgg cgaaaaacgg
     5881 gtaattcctc atccaggcga tgatcctgtg gatgagggcg gctactggat tgagcaagaa
     5941 tcgctcgagt gtcaagaaga tccagtagat gatccagaag gtgatcacga tcggcaggct
     6001 gaagatcagg ccgctgatga gctgattccg taatgagctg aacagaaagc gggggctaaa
     6061 tgggttgggc gttttgacga cggtcgcggg gtaagaccga gcggtttcgc tcggtgtgct
     6121 cgatggtgga ctgtccatga cctaagctcc agatcaagaa gaaacgggat gtcggttccg
     6181 ctgctcgtga cacgagggcg gatattgagg ttagccaggc cagccttcca cggcaattgc
     6241 ccgagcgctg ggccgccgcc acaggctggt agaatgatgc ttggacgcca ggatgcccgg
     6301 ctcgccgacc ggggagtgaa tcggtacact catagagact caaatggtcg gtcggaccgc
     6361 cggccacgtg aagacttgca actctctttc ggcaggttga gaaacatatg accgcacgcc
     6421 aagctgcacc tgagcctacc accgccaaga cggcccttgt gggcggggtg acgctccatc
     6481 tcagtgtccc ggacgcgacc gatcaaaact ggattggtca agacgcgatt ttgcggcagc
     6541 tcttggcgtg ctggctggtg gtagacccgc gcgatctgcc actctcgccg cgcctgatcg
     6601 ggccacccgg aatcggcaag acgacgctcg cgatggcggc cgcccatctg cgtaaacagc
     6661 ctctgtacat caaccagtgt accgccgaca cgcggccgga agacctgatc atcactcccg
     6721 ttctcgccga gagcggcaag atcgcctacc atgcttctcc ccttttgtca gcgatgcttg
     6781 ccggtggcgt gtgcgtgctc gacgagggga accggatgaa tgagaagtcg tgggccagcc
     6841 tcgccccctt gctcgaccac cggcgttatg tcgagagcat tgtcgccggc atcacaatcc
     6901 cggctcatcc cgacttccgg gtctgcgtga cgatgaacga ggacgagtcg acctacgagg
     6961 ttcccgacta catcctgtcc aggctccagc caactctgac gctgggattc ccgacccgcg
     7021 atgacgagat gtccatcctc aaataccact tgccattcgc agaggacgcg cttctggccc
     7081 tgaccgtcga gtttctccag caggcccatc gtctcaagct tgacttctca ccgcgagacg
     7141 ggatcaacct ccttcgcttt gcgatcaaac ggatggccca ggaccccgcc caccctttag
     7201 ggcgcgatgt cgcctggcgc gaggcactgt tttgcgtcct gggcgaagag gccgccgacc
     7261 ttcagaatct agcccgccgc aaacgctcgg cgatgggagg gcagcacgct ccgatgggtc
     7321 tgggagacct tttcttcggc cccgaagatc cactccaccc cgacgccgag tttgaagacg
     7381 aagatgacga ttgatgcgcc tcgcagccgc cgaatgtgcg gcgacgagct cttggagtcc
     7441 tccgcgcctt ggcgggtctg tcaatcgtct gccgacagcc gacttgttgt cccgggccac
     7501 gccgccagga cggcctcgtc aagcgtgggt tggcattcca cgatttctgt cagacgcaca
     7561 ccatgtaaaa cggccatcag ccgagcgtgg gcctggcaga tacggatcgc accccccgat
     7621 cgatcgagcc gcaggtggtg cgccaggagc attccgatga ctttggcatt taagtgccgc
     7681 acgcattcga ggttgaccac aacatggcgc ggagcgttct gctcgtaaag gccatggagc
     7741 cgacttcgaa atagctcgat tgcctcgtcg tcaaccaggt cgctcacctg cggcgtgatt
     7801 acgagcacac cttgaatcac ttcgtccttg atgcggtggt cgggctcgga aaccgcttcg
     7861 tcgcaggcgg gaaaaggctc ggttgcccgg gaatcatgct cgcacgattt ggcaaacgag
     7921 ccctcgcttt ggagccaacc gctgaccatc tcgtcgaccc tggtggtttc cgcacgacgc
     7981 ggtccgactg atagggtggc gataatcggt ccgatctgga tgcggtctcc gtgtttaacc
     8041 acgcgttcct ggtttcgaag cagccggccg ttcaagaccg tgccgttggt gctaccgagg
     8101 tcgtgcagaa ggatgcaatg atcgcggcgt tcgatgacgg cgtggcgctt gctgaccatc
     8161 accgagccca gccgcaggtc gcattcgcgg tcacggccga taacgaatgt atttgaagtg
     8221 accactacgg agcgagactt cgagctgcct acctggacgt caagcacgag ctcgtctttt
     8281 atgcggcttt cagcccgagc gcgcggaacg gtgaacgacg ctgggatctt gggggctgaa
     8341 ggggtcgaaa attcagccgc gtcggagggt actccgcccc gaatcggtgg cagtccggcc
     8401 accgaagtga tcgccgataa gatctcgatg ggcagcgtgc gtgggctcga cgacgcgggc
     8461 cacggacttt cgatcgccga ggcctcgtcg acgtgcaacg catttctcag attgaaaccc
     8521 attgtcggga aaatcgaggc tagctgctga ggaagcccgc aaatcttgag cgccccgccg
     8581 tcggcggact cacaccgcct ttgcgcctcg ccaaccacca aaaccaccca gctcgcgagc
     8641 cgttcgacaa ccgaaaagtt gagcacaacc cgatggttgc cagcctcgat cagatcgatg
     8701 agatcacagg cgagctcgcg gatctgagac tctctgacca gggctttgtc agacaagcgg
     8761 accacggtga ttcggccctt gtagacgacg tgaaacctcg cccagcccag caaggctgtc
     8821 tcggctccct tttccagttg tttttgctgg tgacgccgct tcaagcccgg caggccgtcg
     8881 atcagccgaa tccagggact gtgtggtgtg gatccagccg cttcttcaag gtcgagatta
     8941 ctggcgatcc agttttccat tctgcgatcg aaatcgctcg cttctttctt gggagcggca
     9001 agcaagccga agcgctccga ctcaagcgac gtccggcaag ccggttcttc gcccagcacg
     9061 cgagttggga cggcgaaccg ctcgtcgatg atcgtggccg gtggaatctc ggtttgctcg
     9121 ttcgtcatga acctagctcc tacctcgccg atacaaagat cgcaacttca ggcgcgaagc
     9181 catcatatcg gggcatatgc gggccacgca tgtgcccagt tctaatggtc ggttgcgcgg
     9241 ctgtggtgtg gcgaacgtgc cgcacgcact gcgctagcga cggaagcgtc aaaatgtcac
     9301 gcggtacgcg ttagagctgc gtcaagattg aaacagggca gcacggagac ccgtcgcgtc
     9361 cgatctcaag ggaacaggtg agcaatcgtc actaatcaga actcaagctc cgtagacgat
     9421 tcagcgctcc aataattctc taggcgacat tccggattgg ctcggcccag tttcttgaaa
     9481 atcgccttcg cggcggcggc cggtgcaaaa cctgacgact tcccatgaaa aattgggccg
     9541 gccgcgctcg aaatgtcgct gattgagcgg tgccgtcact gtcatcgtgt ccgagcgagg
     9601 gtctgctagt atacgtcgcc ctggatcgcg gcgggctgat ctgcgggatc agggatgcgg
     9661 gaggctccga gtatctctcg cacagaggtc
//
LOCUS       DATAQL010000533         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3410382, whole genome shotgun sequence.
ACCESSION   DATAQL010000533 DATAQL010000000
VERSION     DATAQL010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3410382"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="VHS97_09175"
     CDS             complement(<1..1906)
                     /locus_tag="VHS97_09175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3448413.1"
                     /translation="MTEPDASTEDSRLIDPADEVLAPSLDSAAALASILDGYMADLQA
                     GRVPDRQQLCDAHPELATQLEACVAGLEFIHRATGPVAETPTTLGEFRIIRELGRGGM
                     GVVYEAEQSSLRRQVALKVLRFGVVADEEAMDRFGREAETVARLHHTNIVPIFSVGCE
                     RGVHYYAMQYIEGRSLADVLMESQRTGNLIPADDVARWGLQAAEALMHAHQRGVIHRD
                     IKPSNLLLDNDGVVWLTDFGLAKRMDEATLAVQGTLMGTPRYMSPEQAASLQQPVDRR
                     TDLYSLGASLYELATGRPLFESAIPHVVIAQILTEEPTRPRQIRPDIPRDLETVLLTC
                     LAKEPLKRYQSAQALASDLRAVTDGRAIQARRSPWAERAVRYVRQRRKTLTGAGLATA
                     ATVLLMLGAVGGWHYYSEWRLGRLVLTTDGPPLAAEVLPASSGDQPMGEPFDVGARTV
                     LTLPAGDYRLRVKAAGLMSQTYRIAVNRGATRTHHLTLDDDRLLGSESIPFSLVTEAV
                     MLTPGKADLIEWNGETLIRRDGSTGKPVWDAARPERPWGRERDPVGAIQRLSRFGDEK
                     RPGQLVQPATDVDGDGTGDLIWAFKGTPSLVALSGKDGSLLWTYSSDPGAVVPRPAAE
                     VFHPGQIMGAA"
     gene            complement(2028..2864)
                     /gene="dapF"
                     /locus_tag="VHS97_09180"
     CDS             complement(2028..2864)
                     /gene="dapF"
                     /locus_tag="VHS97_09180"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX3448414.1"
                     /translation="MRFTKMHGIGNDYVFMSTFDQAWPADPAGLAMVLSDRHRGIGGD
                     GLILIMPSERGDARMRMFNADGSEGEMCGNGVRCVAKYLFDHGLARKQQVAIETGRGV
                     LTLELEISAGRVDKVRVDMGPPILEGGRIPTLLPGNPPVAVPIELSDRVLAVTAVSMG
                     NPHAVVYLADVDTFPLESMGPILERHSAFPKRVNVHIVQVLDKNEVRMRTWERGSGIT
                     LACGTGACAVCVAGVLTGQTARSILAHLPGGDLELSWPDDRSSVFMTGPATEVFSGEW
                     PD"
BASE COUNT          584 a         1023 c         1024 g          540 t
ORIGIN      
        1 ccgccgcgcc catgatttgg ccagggtgaa agacttccgc ggccggccga ggcaccaccg
       61 cgccgggatc ggacgagtac gtccagagga gcgacccatc cttgcccgag agagctacca
      121 gggaaggcgt ccccttgaag gcccagatga ggtcgcctgt gccgtcgccg tcgacgtcgg
      181 tggcgggctg gaccaactgg ccgggcctct tctcgtcgcc gaaacgggac aaccgctgta
      241 ttgcgccgac cgggtcgcgt tcgcggcccc acggccgctc gggccgcgct gcgtcccaaa
      301 ccggctttcc cgtcgatccg tcgcgacgga tgagggtctc tccgttccac tcgatcaagt
      361 cggccttgcc cggagtcaac atgacggctt cggtgacgag cgagaacgga atggactcgc
      421 tccccagcag gcggtcgtcg tcgagcgtca aatggtgagt ccgcgtcgcg ccgcggttga
      481 ccgcgatccg gtaggtttgg ctcatgagcc ccgccgcctt gacgcgcaaa cggtaatcgc
      541 cggccggcaa ggtgaggacg gttcgagcgc cgacgtcgaa cggctcgccc atcggctgat
      601 cgcccgaaga cgcgggcagt acctcggccg cgagcggcgg cccgtcggtc gtcagcacga
      661 gtcgaccgag cctccactcg ctgtaatagt gccagcctcc cacggcgccc agcataagca
      721 agacggtggc cgctgtcgcg agaccggcgc cggtgagcgt cttgcgtcgc tggcggacgt
      781 aacgaacagc gcgttcggcc caaggcgacc gccgcgcctg gatcgcccgg ccgtcggtca
      841 ccgcccgcaa gtcgctcgcc agggcctgag cgctctggta ccgcttgagc ggctccttgg
      901 ccagacatgt cagaagcacg gtctccaaat cacggggaat gtccggacgg atctgtcgcg
      961 gtcgagtcgg ctcctcggtc aggatctggg cgatcaccac gtgcgggatg gccgactcga
     1021 acaggggccg tccagtcgcg agctcgtaca gcgaggcgcc caggctatac aaatccgtgc
     1081 ggcggtcgac cggctgctga agtgacgcgg cctgctcagg gctcatgtaa cgcggcgtgc
     1141 ccatcagcgt gccctgaacg gccagggtcg cctcgtccat ccgtttagct agcccgaaat
     1201 cggtcagcca gacgacgccg tcgttatcga ggagcaagtt tgaaggcttg atgtcgcggt
     1261 ggatcacccc gcgctgatgg gcgtgcataa gggcctcagc agcctgaagc ccccagcgag
     1321 ccacgtcgtc ggccggtatc agattacctg tccgttgtga ctccatcaga acatcggcga
     1381 ggctgcggcc ttcgatatac tgcatggcgt agtagtgcac gccgcgctcg cagccgacgg
     1441 agaagatcgg cacgatgttc gtgtgatgca gccgggctac ggtctcggcc tcgcggccaa
     1501 agcgatccat cgcttcttcg tcggctacaa cgccgaaccg caacactttc aaggccactt
     1561 gacgccggag cgaggactgc tcggcctcgt aaacgacacc catgccacct cgtccgagct
     1621 cgcggatgat ccggaattcg cccagagtcg tcggtgtttc cgcaacgggg ccggtcgctc
     1681 gatgtatgaa ttctaggcct gccacgcacg cctcgagctg ggtagccagc tccggatggg
     1741 cgtcgcaaag ctgttggcga tcgggaacgc gacccgcctg aagatcggcc atgtagccat
     1801 cgaggatgct cgccagcgcc gcggcggagt cgagggaagg cgccaatacc tcatcggcgg
     1861 gatcgataag acgagagtct tccgtcgagg cgtccggctc ggtcatctgg ggtcgctccc
     1921 gcgctgtcat tggttgactg ggaaaggacg tggacgagcc ttcccctcca tgatagggcc
     1981 gagaacttcg cgaatgtcac gcggcggata aaaagaaatc gccgcagtca gtcgggccat
     2041 tcgcccgaga aaacctcggt tgccggccca gtcatgaaaa ccgacgaccg gtcatcgggc
     2101 caggacagct cgagatcgcc accaggcaag tgggccagga ttgagcgggc ggtctgtccc
     2161 gtcaagactc cggcgacgca gacggcgcac gcaccagtgc cacaggccag cgtgatgcct
     2221 gaaccgcgct cccaggtgcg catccgtacc tcgttcttat cgagaacctg cacgatgtgc
     2281 acgttgacgc gtttgggaaa tgcggaatgt cgctcgagaa tcggccccat cgattccagt
     2341 gggaaggtgt cgacgtccgc caggtatacg accgcgtgcg ggttccccat cgaaaccgcc
     2401 gtaacagcca gcacgcgatc gctgagctcg atcggaacgg caacgggcgg gttgccgggt
     2461 aagagtgtgg gaattcgacc tccctccaag atcggcgggc ccatgtcgac gcgaaccttg
     2521 tcgactcgac cggcggatat ctcgagctcg agcgtgagaa ctccccggcc ggtctcgatc
     2581 gcaacttgtt gtttgcgcgc caggccgtga tcaaaaagat acttggcgac gcagcgaacg
     2641 ccattgccgc acatctcgcc ttcggagccg tcggcattga acatgcgcat ccgggcgtcg
     2701 ccccgctcgg atggcatgat caggatcagg ccgtcgccgc cgatcccgcg atggcgatcg
     2761 ctcagcacca tggcgagccc ggcgggatcg gccggccatg cttggtcgaa ggtgctcatg
     2821 aagacgtagt cgttgccgat gccgtgcatc ttggtgaagc gcaaggtcct gttccagtca
     2881 tgcggggaga ttttaaaagc aggttacacc tcagggggtg gctggggtcg acgcccgcag
     2941 ggcgacgccc gcagatccga ggctgccgcg ccgggggctc gctagctatc gccagctcga
     3001 ccccagccac ccagcacttg ttatctgcga agatgctaaa gttgaacgat gccagatttc
     3061 cgccacttca ttcgacgtat catcgatcgg acaccggcct tggcgcttcg ttcgacaata
     3121 acagcgacgg gtactcgtca caagtcgatg aaccgtgccg atgacagcgg a
//
LOCUS       DATAQL010000534         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1579195, whole genome shotgun sequence.
ACCESSION   DATAQL010000534 DATAQL010000000
VERSION     DATAQL010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579195"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VHS97_09185"
     CDS             complement(<1..437)
                     /locus_tag="VHS97_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448415.1"
                     /translation="MGHGHQSRPDHGAGPGRLADGDSNWRWLFFINVPIGVLAFLGMA
                     SSLPATTTHKSRFDFFGFTSLSIGLGALQLMLDRGQIKDWFASTEIWIEGATALAAFY
                     LFVVHMLTTTGQRFINPVLFKDRNFIAGSLIFFVAGLVMFATLA"
     gene            627..782
                     /locus_tag="VHS97_09190"
     CDS             627..782
                     /locus_tag="VHS97_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448416.1"
                     /translation="MRPIRPASQPLSGIMMADAMMYDVSTQAIWSWVAENEIKVRRGL
                     SSSTAPA"
     gene            complement(885..1274)
                     /locus_tag="VHS97_09195"
     CDS             complement(885..1274)
                     /locus_tag="VHS97_09195"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX3448417.1"
                     /translation="MFKVMWLLRRKPGISHEQFKEHYESSHVIMAQKYIGHLLIEYKR
                     NYKTETWGGGVPTDTGGGFGLIDWPYDCVTEWVMKDESALDRINAIFAHPVIGKEFHD
                     DEANFLDRDSVVLIKCQVIDTGPGRSA"
     gene            1483..2052
                     /locus_tag="VHS97_09200"
     CDS             1483..2052
                     /locus_tag="VHS97_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448418.1"
                     /translation="MMTRIPVLQPFGRQDAVIPHVYRPRMAAAMEQLSAAVYQETTLS
                     YREAEGARIRIAHINGCLMCQGYRIAEHLPAALQKMDSAEAPNTGEKRGPPPTEEYYK
                     AVAADWRASEMFSDRERLAIEYAERISVDPVPLPYDDDFWGRLHAHFDEGEIADLTYS
                     ITTWIATGRVVHALGLDGACAIQKSTHRA"
BASE COUNT          471 a          680 c          669 g          417 t
ORIGIN      
        1 gccaaagtcg cgaacatgac caggccggcg acgaagaaaa tcaggcttcc ggcgataaag
       61 tttcgatcct tgaaaagcac cgggttgata aagcgctgcc cggtcgtcgt cagcatgtgg
      121 acgacaaaga ggtagaacgc ggcgagcgcc gtcgcgcctt cgatccagat ctcggtcgaa
      181 gcgaaccaat ccttgatttg gccgcgatcg agcatcaact gcagggcgcc caagccgatg
      241 ctcagggagg tgaagccgaa gaaatcgaag cgcgacttgt gagtcgtcgt cgcgggcagc
      301 gagctcgcca tcccgaggaa tgcgaggacg ccgatcggca cgttgatgaa gaaaagccag
      361 cgccagttgg agtctccgtc agccagccgc ccagggccgg ccccatgatc gggccgagac
      421 tggtggccat ggcccacatc gacatcgccc gcggttgacg cgccggcggg gttatgtcga
      481 gcagagccgc ctgtgacagc ggcaccagcg cagcgccgct cacgccttgc agcaggcgag
      541 ccattacaat ctgcggcaaa gactgcgcaa gtccgcaaag catcgacgcg agggtaaaac
      601 ccgctacgga gtagaggaat acctgtttgc ggccaatccg gcccgcaagc cagccgctca
      661 gcggaatcat gatggcggac gcaatgatgt acgacgtgag cacccaggcc atctggtcct
      721 gggtcgcgga gaacgaaatc aaagtacggc gcgggctgtc gagctcgacg gcgccggcgt
      781 gagcgccgtc gctcaccgaa gacgtcgccg cgctcgaggt cccaggtggc gacgattcga
      841 tctgcggtct cccgaatgat gagactgagg cgcgcgcgcc tgaatcatgc cgatctgccg
      901 ggacctgtat cgatgacctg gcatttgatc aaaacgaccg agtctctatc gaggaagttc
      961 gcctcatcgt cgtgaaactc cttaccgatc accgggtggg cgaaaatggc gttgatcctg
     1021 tcaagggccg attcgtcttt catgacccac tcggtgacgc agtcgtacgg ccagtcgatg
     1081 agaccgaagc cgccccccgt gtcggtaggc accccgccgc cccaggtttc ggtcttgtag
     1141 ttgcgcttgt actctatcaa cagatgtcca atgtactttt gagccatgat cacgtggctg
     1201 ctttcatagt gctctttgaa ttgttcatgg ctaatccccg gcttgcggcg aagcaaccac
     1261 attaccttaa acattgtcca ttacctctcg ttcagttttg gtagtagccg actcgagtgc
     1321 gatcggcagg cttcgcgagg actctcacgg gcatactaat ccaccgcgcc acccgtttga
     1381 agaccggctg tccgatgaga tcaacaccgc agtcttgccg cgtgtatcat cgagctccct
     1441 tcgagcacgg cgccaaaaca tgacgaaccg gaggagcaga gtatgatgac ccgtatcccc
     1501 gttctccagc cgtttggccg acaagacgcg gtcattccgc acgtgtaccg gcccaggatg
     1561 gcagctgcga tggaacagtt gtcggccgct gtctaccagg aaacgacgct cagctatcgg
     1621 gaagcggaag gcgcgcgtat ccgcatagcc cacatcaacg gctgcctcat gtgccagggc
     1681 taccgcatcg ccgagcacct gccggccgcg cttcaaaaaa tggactccgc cgaggcgccg
     1741 aacaccggcg aaaagcgcgg cccgcccccg acggaggagt attacaaggc tgtggccgcc
     1801 gattggcgag cttcggaaat gttttcggac cgagagcgcc tggcgatcga gtacgcggag
     1861 aggatctccg tcgaccccgt gcccctcccg tatgacgacg acttctgggg acgtctgcac
     1921 gcgcatttcg acgagggcga gatcgccgac ttgacgtaca gcattaccac ctggatcgcg
     1981 accgggcggg tggtacacgc actgggcctc gatggcgcct gtgcgatcca aaaatcgacg
     2041 catcgcgcgt aggcgccgcc gccggtcaca atatcccctg acccggtgga taacccagtg
     2101 cacaaccggg tctgagtcac ccaatcgtca cggaatttca gtcggttatg ttgaactggc
     2161 gaatagttga gcagtctatt ttcgtcgtga tctgcgtcga gtctgtttag aggtcgcgcg
     2221 cattttcttc ttgactt
//
LOCUS       DATAQL010000535         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3410505, whole genome shotgun sequence.
ACCESSION   DATAQL010000535 DATAQL010000000
VERSION     DATAQL010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3410505"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VHS97_09205"
     CDS             <1..524
                     /locus_tag="VHS97_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448419.1"
                     /translation="HIVHFSGHGNIDDEIILESGEGELAFPNGTKSSGRDMQRLEPVH
                     LASGVCAPKPLGKSALVDVLKACNEGNIRVVVLNACHTRPHAEALSEIIDCTISMNRA
                     ISDVGAIKFAASFYGALAFGRSVKKAFDQGLARLNAEGVSETGIPELVVRAGVNASTL
                     VLVGPPGNRAEVN"
     gene            complement(847..920)
                     /locus_tag="VHS97_09210"
     tRNA            complement(847..920)
                     /locus_tag="VHS97_09210"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(884..886),aa:Val,seq:gac)
     gene            complement(981..1541)
                     /gene="frr"
                     /locus_tag="VHS97_09215"
     CDS             complement(981..1541)
                     /gene="frr"
                     /locus_tag="VHS97_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX3448420.1"
                     /translation="MSIEEIALEAEERMEKSVTLLADQLRGIRTGRANVGLVESIRVD
                     YYGSSTPLKQMANLSTPEPQQILIRPFDPSIIGDIVKAIQTSDLGLSPNSDGKVVRLN
                     VPPLSVEQRKKLANRVKELAEEARVAIRNIRRDANKQADVEQTDKILTEDDLTTCKDE
                     VQTLTKKFEAKVNEAADKKSAEIMEV"
     gene            complement(1725..2489)
                     /gene="pyrH"
                     /locus_tag="VHS97_09220"
     CDS             complement(1725..2489)
                     /gene="pyrH"
                     /locus_tag="VHS97_09220"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX3448421.1"
                     /translation="MSSSTAAGPPVFRRILLKLSGESFCEPGKGGISVEEISRIARQT
                     SQIAARGVQLAIVVGGGNILRGAQFSRGSDVIKEATAHYMGMTATVINGLALQDALEH
                     EGCATRLLTTIRMDEVAEPFIRRRALAHLDRGRVVILATGTGSPFVTTDTAAALRGKE
                     LGAEALLKATRVDGIYSADPEKNPHAVLYQQLTFEQVMREGLTVMDMTAIGMCLENQL
                     PILVFNFKKEGNIERAIAGEPIGTWVSNTPRPRPGS"
     gene            complement(2609..3205)
                     /locus_tag="VHS97_09225"
     CDS             complement(2609..3205)
                     /locus_tag="VHS97_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX3448422.1"
                     /translation="MADITAQAVNEFRKKTGLGLMECKALLKEADGDLKKAETLAKER
                     GLKQAELRAGRAATAGRVEVYIHHDAKSGTMVELNCETDFVARNEEFKQLAKDLALHI
                     MAANPLCVRREDVPEPVITEQKRIFMSQVADKPQNIQEKIAEGKLNAWFAESVLLDQK
                     FVKDETKSVRDVILAVNSRTGENISVARFARFKVGEEK"
BASE COUNT          588 a          958 c          977 g          737 t
ORIGIN      
        1 cgcacatcgt ccacttcagc ggccacggca acatcgatga tgagatcatt cttgagagcg
       61 gggaaggaga acttgcattt ccgaacggca cgaagtcgtc ggggcgtgat atgcagcgtt
      121 tggaacccgt acatcttgcg agcggcgtct gcgctcccaa gccactgggc aaatccgcgc
      181 tggttgacgt acttaaggcg tgcaatgagg ggaacattcg cgtcgtcgtg ctcaacgctt
      241 gtcacacgcg tccccatgcc gaggcgctct ccgaaatcat tgactgcacg atcagcatga
      301 atcgagcgat cagcgacgtg ggggctatca aattcgccgc ctcgttctac ggtgccctgg
      361 ctttcggccg ttcagtcaag aaagccttcg accagggcct ggctcggcta aatgccgagg
      421 gtgtctcgga gaccgggatc ccagaactgg tggtccgcgc tggtgtgaac gcctcgacgc
      481 ttgttctcgt cggcccccct ggcaatcgtg cagaggtaaa ttgatgacgc tttacgttag
      541 gcggccgtct acgcgaagaa gtaagatccg ttcggcaaac tgattacatg gtcgttcgga
      601 aagagaattg ccgctgtgat tatcctagta attgagttaa tgtttactta tgctgccgta
      661 atggggcaat tctctccgtt ggtccgctaa acatcagttg gcgccttccc aaggagcctc
      721 gcaatcagtt tgccgacctc ccgtactcgc caacgatgag cacagtgcgc ggacgagggt
      781 cggtgagggt aaccggaccg tccgccatgg gaagcgtggc aatgatctcg gcatagtgct
      841 tgacaatggg cgatacaggg ctcgaacctg tgacctcatg cgtgtcgagc atgcgctcta
      901 gccaactgag ctaatcgccc cgcgtttgtc tagtttccgt tataggtgaa cgacggacat
      961 gcggtcaagc cggtgtcacg tcagacttcc atgatctcgg cggacttctt gtcggccgct
     1021 tcgttaacct tggcctcgaa cttcttggtg agtgtctgga cctcgtcctt gcacgtcgtc
     1081 agatcgtctt cggtgaggat cttgtcggtt tgctccacat ccgcctgctt gttggcgtcg
     1141 cgacgaatgt tgcggatggc tactcgggcc tcttcggcca gctctttgac acggttggct
     1201 agcttcttgc gctgctcgac cgacaacggg gggacattca accggaccac cttgccgtcg
     1261 gagttgggag aaagacccag gtcgctggtc tggattgcct tgacgatatc gccgatgatc
     1321 gagggatcga acggtcgaat caagatctgc tgcggctcag gcgtgctcag gttggccatc
     1381 tgcttgagcg gggtcgaaga tccgtaatag tcgactcgga tcgattcgac gagccccaca
     1441 ttggcccggc cggtccggat gccgcgcaac tgatcggcca gcagagtcac gctcttttcc
     1501 atacgctctt ccgcttcgag cgcgatttct tcgatgctca tgatccactc acccttttgc
     1561 ttgaggacag agccaatcct cgcaaagtcg ttcgtgtctt gtcgcggcgc gaaatgtcgc
     1621 cgtcactctg ccatcataac acgatcgcgg tcgaccgaaa ggtgactctt gcgtagccgc
     1681 gaatcgcggt agaaacgccc gactgccaga gtggctggcg cggctcatga gccgggtctg
     1741 ggccgagggg tgttgctgac ccaagtgccg atcggttctc ccgcgatggc tcgttcgatg
     1801 tttccctctt ttttgaaatt gaacaccagg atcggcagct gattttcgag gcacatgccg
     1861 atggccgtca tgtccatcac tgtgaggccc tcgcgcatga cctgctcgaa cgtgagttgc
     1921 tgatagagca cggcgtgcgg gtttttttca gggtcggccg agtagatgcc gtcgacccgc
     1981 gtcgccttga gcagggcctc ggcgccgagc tccttgccgc ggagcgcggc tgcggtatcg
     2041 gtggtgacga acgggcttcc cgtgccggtg gccaggatga cgacgcggcc gcggtcaagg
     2101 tgggccaagg cgcggcggcg aatgaacggc tccgccacct catccatgcg aatcgtggtc
     2161 aagagccgcg tggcgcagcc ttcgtgctcg agagcgtctt gcaaagcaag gccgttgatc
     2221 acggtcgccg tcatacccat gtaatgcgcg gtcgcctcct tgatcacgtc ggatccgcgc
     2281 gaaaattggg cgccgcggag gatgtttcca ccccccacga cgatggccag ttgaactccc
     2341 cgggcggcga tctgcgaggt ctgccgtgcg atccggctaa tttcctcgac gctaatgccc
     2401 cctttacccg gctcgcaaaa gctttcaccg gaaagcttga gcaagatgcg gcggaaaacg
     2461 ggcgggccgg cggcggtgct agagctcatg aagcagaatc ctgttggtga ctcgcgaatc
     2521 ctcaatgaga ggaatgtcga ttccggaccc cgcttcggga cttccggctg ccgtggatgc
     2581 cgtctcgatg tcaactgcga tcgatcgatt acttctcttc gccaaccttg aacctggcga
     2641 accgggcaac cgagatattt tcaccggtcc gggagtttac agccaagatg acgtcacgga
     2701 cggacttggt ttcgtccttg acgaacttct ggtcgagcag gacgctctcc gcgaaccacg
     2761 cgttgagttt gccctcggcg attttctcct ggatattctg tggtttgtcg gccacctgag
     2821 acatgaagat ccgtttttgc tcggtgatga ctggctcggg cacatcctcg cgccgcacac
     2881 agagcgggtt ggcggccatg atgtgcagcg ccaggtcttt ggccagctgc ttgaactctt
     2941 cgttgcgcgc cacgaaatcg gtctcgcagt tgagctcgac cattgtgccg gacttggcat
     3001 cgtgatgaat gtagacctcg acccggccgg ccgtggcggc gcggcccgcc cggagctcgg
     3061 cctgcttgag accgcgttcc ttggccagcg tctcggcctt ttttaagtct ccgtcggctt
     3121 ccttcaagag tgccttgcac tccatgagtc ccagcccggt cttcttgcga aactcattaa
     3181 cagcctgagc cgtgatatcg gccatcggcg cgactcctca ttttcgtgcg ggcgcgagat
     3241 gtcaatcacg cgcccgcgtc
//
LOCUS       DATAQL010000536         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5397606, whole genome shotgun sequence.
ACCESSION   DATAQL010000536 DATAQL010000000
VERSION     DATAQL010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5397606"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3537
                     /locus_tag="VHS97_09230"
     CDS             <1..>3537
                     /locus_tag="VHS97_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3448423.1"
                     /translation="DGATEVEVVDLEAARRNAGRWKVLAPRLRVAAQLVHLSAIRMLE
                     LELDHDRPYAVLEWVGATTLAAWVAANGPKSRQEAIALSRDLADALRAAHRLGLPHGQ
                     LGPDQVLLVGGTKPKLDFSSAAVGFPCGPEPIKSRDRAPDAHAPNEAAARAADLYSLG
                     ELLAWLLTGRGDRFNDKPTGTGLSGGSSLGDLVHALLAVDPAERPSAREVQERLDELL
                     APMDATERWTMPVERSPEARHFLQTERFDPIDATDGSKTLVLNPPVTRLGRYRLLNKL
                     GEGAQGVVHRAEDPSDGSIVAIKILRTDRIGSHKVIRRFRKEARLMAEANNPYVVNLL
                     EFNEDDGVPYMVLEFVAGESLAHLLAEQSRLGEREALSIMAAVARGLMQAHERGIVHR
                     DIKPSNILLLEPRQPSATQKTEPDGFVSNGEHEPRLLSDFNATTLASTSKGDIDTEAA
                     RPRVKLSDFGLARHIVDSESMALTAAGAILGTPHYMAPEQWTGRAVDCRTDVYAMGAT
                     FFHLLAGRPPFVAETRDELAAQHCNEPPPRLGTLNAEVSDGVARLIDRALSKHPEDRH
                     ADAGAILRDLEALLEGRPTDLAIHPRLPEFDPKRVAQFEFRWELDSSPRQLWPLVTNT
                     DRLDRAIGFAPVNYTTRYEEGRGARTFIEGRKAGMVEVGEEHPYEWIEPRRMGVLREY
                     SEGPFRWLVSVVELLDGPRGGTTLIHRLILEPSSWKIRVGSRWGVGVTLRKSLARVYK
                     RIDATVKSQRQRGISPADDAFAEPDGMPAPRRQRLERLLDRLIEQGADAEVVDRLGEY
                     LALGPAQELARIRPLALAERWRLEPQKVVAACLWGAHAGLLELHWDLLCPVCRISCQV
                     TDTLRAIAEHAHCAACNLDFQLDFARSIELIFRVHPEIRKADLGVYCVSGPAHSPHVL
                     AQIRVAPLERIELELELAAGNYRVRGPQLPWSLDFRVQSTATIRRWDIDLAAAPTSGQ
                     VEALRAGGQILILKNPHPQELVVRVERTAISADALTAARATSMALFRELFPGEILAPG
                     QLATVSMVTLMVTALDPEQADTLYRDLGDTGAFGVVHEHLQCLGAAIRAGGGAVVKTM
                     GEGVVASFADVTSAVMTAVDLLPQLARGAATRPLRLRVAVHRGTTVAATLNDQLDYFG
                     TTARQTIQILDDARGDELVLTQAVAADPEVAG"
BASE COUNT          644 a         1150 c         1121 g          622 t
ORIGIN      
        1 aggatggtgc gaccgaggtc gaggttgtcg atctcgaggc cgcacgcagg aatgccggcc
       61 gctggaaagt gctggcaccg cggctccgcg tggcggccca gctcgtgcat ctgtccgcga
      121 ttcgcatgct cgagctcgaa ctagatcacg accggcccta cgctgtcctg gaatgggttg
      181 gtgcgacgac acttgctgca tgggtggcgg ccaacggacc caagtcccgt caagaagcaa
      241 tcgcgctcag tcgtgatctg gccgatgccc tgagggccgc tcatcggctg ggactgccgc
      301 acggccagct cggtccggac caagtgcttc tggttggcgg aacaaagccc aagctcgatt
      361 tcagcagcgc cgcggtcggg ttcccgtgcg ggccagaacc catcaagtct cgcgatcgcg
      421 cccccgatgc ccatgccccg aatgaggccg ccgctcgagc ggccgacctc tacagcctgg
      481 gtgagctcct cgcctggctg ttaaccggac gcggcgatcg attcaatgac aaacccactg
      541 gcaccggcct ctcaggcggc tcgtcactcg gagatcttgt ccacgccttg ctcgccgtag
      601 atccagcaga gcgccccagc gcgcgcgagg ttcaagaacg gctcgatgag ctcctcgccc
      661 ccatggatgc gacggaaagg tggactatgc ccgtcgaacg ctcccctgag gcgcgccatt
      721 ttttgcagac ggagaggttc gatccgatcg acgccactga cgggtccaaa actctggtac
      781 tcaacccgcc ggtgactcgc cttggccggt atcgcttgct caacaagctg ggcgaaggag
      841 cccagggagt ggtccaccgc gcggaggatc cgtccgacgg ctcgatcgtc gcgatcaaga
      901 tcctgcgaac tgaccggatc ggcagtcaca aagtcatcag gcggtttcgc aaggaggccc
      961 ggctcatggc cgaggccaac aatccctacg tcgtcaacct gctggaattt aacgaggacg
     1021 acggcgtccc ttacatggtc ctggaattcg ttgccggtga aagcctcgct catctgttgg
     1081 ccgagcagag ccggctgggc gaacgagagg ccctctcgat tatggcggcc gtggcgaggg
     1141 gtctgatgca ggcccacgag cggggaatcg ttcatcgcga catcaagccc agcaacatcc
     1201 tcctcctcga gccacgccaa ccttccgcta ctcaaaagac cgaaccagac ggattcgtct
     1261 ccaatggaga acacgagccc cggttattgt ctgatttcaa cgccactact ctggcatcga
     1321 cgtcgaaagg agacatcgac acggaggcgg ccaggccgcg ggtaaagctg tcagatttcg
     1381 ggctggcccg gcacatcgtc gatagcgaat cgatggcgct gaccgcggcc ggagccatcc
     1441 tgggaacgcc gcactacatg gcgccagagc aatggaccgg ccgcgccgtc gattgccgca
     1501 cggacgttta tgcaatggga gcgacgttct ttcatctctt ggccggcaga ccgccattcg
     1561 tcgcagagac acgggacgaa cttgccgcgc agcactgtaa cgaacctccg ccgcggctgg
     1621 gaactctgaa tgcggaggtc agtgacggtg tagcgcggtt gatcgaccgc gccctctcca
     1681 aacaccccga ggaccgtcat gccgatgccg gggcgattct ccgcgacttg gaagccctgc
     1741 tggaaggcag acccaccgac ctggcgatcc accctcggct tcccgagttc gacccaaagc
     1801 gggtcgccca attcgaattc cgatgggagc tggattcatc gccgcggcaa ctctggccgc
     1861 tcgtgaccaa tacagaccgg ctcgatcgag cgattggatt cgcaccggta aactacacga
     1921 cccgctacga ggaaggccgg ggcgcgcgga ctttcatcga ggggcgcaag gccggcatgg
     1981 tcgaggttgg cgaagagcac ccgtatgaat ggatcgagcc gcggcggatg ggagtgcttc
     2041 gcgaatatag cgagggtccg ttccgatggc tagtgagcgt tgtcgagctc ctagacggtc
     2101 caaggggcgg cacaaccttg atccaccggc tcatcctcga gccgagctcg tggaaaattc
     2161 gagtcgggtc gcgctggggt gtcggcgtca ccttgcgcaa gagcctcgct cgggtctata
     2221 agcgcatcga tgcgacggtg aagagccaaa ggcaacgcgg catttctccc gcagacgatg
     2281 catttgcaga gccggacggg atgccggctc ctcggcgtca gcgcctggaa cgcctgctgg
     2341 atcgactgat cgaacaaggc gccgatgcgg aagtggtcga tcggttgggc gaatacctgg
     2401 ccctcggccc ggctcaggag ctggcccgca tccgcccact cgctctggcc gagcgctggc
     2461 ggcttgaacc ccaaaaagtt gttgccgcgt gtctgtgggg cgcccatgcc ggcttgctcg
     2521 agctgcactg ggatctgctc tgtcccgtct gccgcatctc atgccaggtc accgacacgc
     2581 tgcgtgcgat cgccgaacac gcccactgcg cggcttgcaa cctcgacttt caactcgact
     2641 ttgcccggtc gatcgagctg attttccgcg tgcacccgga gatccgcaag gccgatctgg
     2701 gcgtctactg cgtcagcggc ccggcacact cgccgcacgt gctggctcag atccgcgtcg
     2761 cgcctctgga gcgcatcgag cttgagcttg agctagccgc aggtaactac cgcgttcgag
     2821 gcccgcagct accgtggtcg ctcgactttc gggtccaatc gaccgcgacg atccgccggt
     2881 gggatatcga cctggccgcg gcgccgacat cgggacaagt cgaagcgctc cgcgctggag
     2941 ggcagatcct tattctcaaa aacccccatc cgcaagagct ggtggtccgc gtcgagcgga
     3001 cagcgataag cgcggacgcg ctcaccgccg cgcgggccac gtcgatggca ctgtttcgcg
     3061 aactctttcc tggcgagatt ctcgcgcctg gccagctcgc cacggtctcg atggtgactc
     3121 tcatggtcac cgcgctggat ccagagcaag ccgacacgct ttaccgcgac ctgggggata
     3181 cgggtgcgtt tggcgtggtc cacgagcacc ttcagtgtct gggcgcagcc atccgcgccg
     3241 ggggcggcgc cgtggtcaag acgatggggg aaggcgtggt ggcttcgttt gccgacgtga
     3301 ccagtgcggt aatgaccgcg gtcgatctgc ttcctcaact tgcgaggggc gcggcaacgc
     3361 gcccgctgcg tctgcgggtc gcggttcatc gcggcacgac ggtggccgcc actctcaacg
     3421 atcaactcga ctatttcggc acgacggccc gccagacaat tcaaattctc gacgatgcgc
     3481 gcggggacga actcgtcttg acccaggctg tcgccgccga tcccgaggtc gccggcc
//
LOCUS       DATAQL010000537         4378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1122183, whole genome shotgun sequence.
ACCESSION   DATAQL010000537 DATAQL010000000
VERSION     DATAQL010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122183"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VHS97_09235"
     CDS             complement(<1..1003)
                     /locus_tag="VHS97_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3448424.1"
                     /translation="MHKLRILILYPDAGGLALLTSMLKSLGHIIEEATNDRMAVRLME
                     RNNIDLVLAGVDPLDAEALELLTYVRRKHREVPVILLFPRLHPERAKEALRLGAMAVL
                     KYPVPAAELRAAVLQALELCDAKAPSEPAGQPPAATVASTTAKPATPSTTVIHDGQPP
                     AAPRPMAINPILSTGNSGTAGGVGGLAPSGSSLPRIEQLTREIGLIGNDPGWRQVVDL
                     AATIASTRTSVLIVGEPGTGKSLLARLIHAIGPNSERPFITVEASALAEELSNPEPAD
                     AVYPPRVNDPQAWTSKVAQAKGGTLYLDEVAAMPLEFQLNLLRDLHFRDYEASAGHSL
                     "
     gene            complement(1543..2661)
                     /locus_tag="VHS97_09240"
     CDS             complement(1543..2661)
                     /locus_tag="VHS97_09240"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX3448425.1"
                     /translation="MRGLPMLITTVLSTQCCHESSRKTVHRECMLTRMRPWKTGSRCI
                     LHFRGRDQPQIAERIKDAVTVTTRKPDSDVRTWLMHSSVSAFLNHLRNERQASGHTLR
                     SYEHDLGLYCDYLSEAQGEGADPRAAQPNRLRRYSAWLSGQGFAASTVARRLASLRSY
                     FRYLRRQGVVESDPSVGLRNPKQSKRLPRLLRVDEVIKLLDSVPIDTPPGVRDRAMLE
                     TLYGGGLRVSELVGMNLDDLDFEQELVRVRGKGRRERLCPVGPMAVHWIKCWIPVRHP
                     KHAYEKAVFLNQRGSRLTTRSVGRLLEAQLLRAGLVKNASPHTLRHSFATHLLDRGAD
                     LRSVQELLGHRNLTTTQIYTHVTQERLLDIYHEAHPRA"
     gene            2793..2865
                     /locus_tag="VHS97_09245"
     tRNA            2793..2865
                     /locus_tag="VHS97_09245"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2826..2828,aa:Met,seq:cat)
     gene            3093..3557
                     /locus_tag="VHS97_09250"
     CDS             3093..3557
                     /locus_tag="VHS97_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA hydrolase"
                     /protein_id="HEX3448426.1"
                     /translation="MAQSQSNDTETLAHHLILPRDANHHGTLYAGVLLSMALEAGYAT
                     AFRAAGLNANLVLKRVLDLRCYEPVPIGRVVQIRGREIHRARAQIVVGLLGSSKADQP
                     SFWMDGLMQFVQVDADGRPVPLEDEPEDDSLMLDIPWSPLRDRARKLLQIRQ"
     gene            3788..>4378
                     /locus_tag="VHS97_09255"
     CDS             3788..>4378
                     /locus_tag="VHS97_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3448427.1"
                     /translation="MTRLRGTSSGVLFCQLERLFAQGTASGLTEGELLERFVSRRDEA
                     AFEALVARHGPMVLGVCRHLLRDPNDVDDAFQATFLVLVKKASTVRRCELLGNWLYGV
                     AFRVASRARALAARRTARFVSGDYVVENLTAAVCRDDGGLDPKTLLDQSPWLHQEVCH
                     LPDKYRTPIVLCYFEGLTHDEAASRLGWPLGTVKGRL"
BASE COUNT          859 a         1275 c         1337 g          907 t
ORIGIN      
        1 acaaggaatg gccggcgctt gcctcgtagt cgcgaaaatg caggtcgcga agtagattga
       61 gctgaaactc gaggggcatg gcggccactt cgtcaagata gagcgtgccg cccttagcct
      121 gggccacctt gctggtccac gcctgagggt cattaaccct aggcggataa accgcatcag
      181 caggctcagg gttcgacagc tcttcggcca gcgccgaggc ctccacggtg atgaagggcc
      241 gctcggagtt gggcccaatc gcgtgtatca aacgcgccag gagcgacttg ccggttcccg
      301 gctcgccgac gatcaagacc gaggtgcggg tcgaggcgat cgtcgccgcc aggtcgacga
      361 cctgccgcca gcccgggtcg ttaccgatca aaccaatctc gcgagtgagt tgctcgatac
      421 gcgggagcga agaccctgac ggcgccagtc cgcccacacc gcctgctgtg cctgaattgc
      481 cagtcgacag aataggattg atcgccatcg ggcgcggcgc ggccggcggc tgcccgtcgt
      541 ggattacggt cgtcgacggg gtcgcaggtt tggccgtggt agaagccaca gtggcggctg
      601 gaggctgccc agcgggctct gacggcgcct tggcgtcgca caactcgagc gcttgcaaca
      661 cggcggcacg aagctcggcc gcagggacgg ggtatttgag gacggccatc gcgcctaatc
      721 gcagcgcctc ctttgcccgc tcgggatgaa ggcgaggaaa caagagaatg acgggaacct
      781 ctcgatgttt gcgcctcacg taggtcaaga gttccagagc ttcggcgtca agcggatcaa
      841 ctcccgccaa gaccaggtcg atgttgtttc gttccatcag ccggaccgcc atgcggtcgt
      901 tggtggcttc ctcaatgatg tgacccagcg acttcagcat cgaggtcagc agagcgagcc
      961 ccccggcgtc gggataaagg atcaagattc ggagcttgtg catggaattc atctctcagc
     1021 gtgcgttcga gttcaattgc acgaatgcgg tgggtcctcc ctgaatgacc tgcggatcac
     1081 cctcaggccc ggcctggtga ttcgcttcac gcgttcgcgg gacgagacgc gaaaaggcaa
     1141 cagctcgctg tccccatctc atccgagcga ctcgacatcg ccgacttgac cgcttcaggg
     1201 ccgcgtcagg cgatagagtg gtgtcgttct agagaaacgt gtatgtcatg gatgcaacat
     1261 cgtcaaaaag atgattcccg gcctccgcca atttcgcttg atgatgaagc agtgtcgccc
     1321 gattatcgat cgcgatcgcg gacttcggcg taggttggaa atcggggtca tgggactctg
     1381 tgaaagttaa atggccaagc aagtaccgcg gtccgccacg gatcggggtg attgtcgagc
     1441 cgagcccgcg ccagagatgc tttgtgatcg atttttgatg atacggtcgg gacgtcagtc
     1501 gctgacgcac aaagacgttc cgctgtttcc aacggtcgta aatcatgcgc gcgggtgtgc
     1561 ctcatgataa atatcgagta atcgctcctg cgtgacgtgg gtgtagattt gagttgtcgt
     1621 caaattccgg tgcccgagga gctcttgcac gctgcggaga tcggctcccc gatcgagtag
     1681 gtgggtggcg aagctatggc gcaatgtgtg tgggctggcg ttttttacta gccctgctcg
     1741 caataactgc gcctccagca atcgaccgac gcttcgggtg gtcagccgcg agccacgctg
     1801 gttgaggaaa acggcctttt cataagcgtg cttcggatgc cgcaccggta tccagcactt
     1861 gatccagtgc accgccatcg gaccgaccgg gcaaagccgc tcgcggcgac ccttgccccg
     1921 aactcgaacc aactcttgct cgaaatcgag atcgtccagg ttcatgccca ccaattcgct
     1981 gacgcgcaac ccgcccccat acagggtttc cagcatcgcg cgatctcgaa ccccgggcgg
     2041 tgtgtcgatc ggcaccgagt cgagcagttt gatgacctcg tcgacgcgga gtaatcgagg
     2101 taaccgctta gattgcttag gattacgtag accaaccgac gggtccgatt ccacgacacc
     2161 ttgtctgcga agatatcgga agtatgagcg taagctcgcc aaacggcgtg ccaccgtgct
     2221 ggcagcgaac ccttgcccgc tcaaccaggc cgaatagcga cgcaaccgat tcggttgggc
     2281 ggcccgtggg tcggccccct ctccctgggc ctcgctcaaa taatcgcaat acaagccaag
     2341 atcgtgctcg taagaccgta gcgtatggcc cgacgcttga cgttcgtttc tcaaatgatt
     2401 caggaaagcg ctgacagagc tatgcatcaa ccaagtcctc acgtcggaat caggcttacg
     2461 agtcgttacc gtcactgcgt ccttaattct ttcggcgatt tgcggctggt ccctccccct
     2521 gaaatgcaat atgcaacgcg acccggtttt ccatggtcgc atgcgtgtca gcatgcactc
     2581 tcgatgtacg gttttgcgcg aagactcgtg acagcattgt gtcgacaaga ccgttgtaat
     2641 gagcatcggc aaccctcgca tggaccgttc cgccagggga agcgatcttt tccgcaagaa
     2701 aagccgttgg gccgcatagg actgaggccc attttagggt gcttccgtgt gaatgccctt
     2761 tttctttgaa gaagtccgcg ttagtattga ggtgcgccgt ggagcagcgg ctagctcgcc
     2821 aggctcataa cctggaggtc gtaggttcga ttcctaccgg cgcaattaaa tcgactgcgg
     2881 tttccgctca ttaaaccccc gctcacgcgg ggttttttgt ttcgctggcc ggaaaaaggg
     2941 cggaattgat gcgcccgagc gactccgacg ttgagccccg ggccggcacc gatgggaacg
     3001 gttgcgaccc tcttgtccgc gtgctaagct gaccccaatg tcgctcgagc aagtcagcag
     3061 aagtctttcc ccaaccggcg gactcgacgc gcatggccca atcgcaatcc aacgataccg
     3121 agacattagc ccatcacctc atcttgccgc gcgacgccaa tcatcacggg acgctctacg
     3181 cgggagtttt gctttctatg gccctcgagg ccggctatgc gaccgcgttt cgggccgcag
     3241 ggttgaatgc caacctggtc ttgaagcgcg tcctcgacct gaggtgttac gaacctgtcc
     3301 cgatcggacg cgtcgttcag atccgcggcc gcgaaatcca tcgcgctcgg gcccagatcg
     3361 tcgtgggtct cctgggttcg tccaaggcgg atcagccgtc gttctggatg gacggcctga
     3421 tgcagtttgt ccaggtcgac gcggatggtc ggcccgtacc gctggaagac gagccagaag
     3481 acgatagcct catgctcgat atcccctggt cccctttacg ggatagggct cgaaagctcc
     3541 tgcaaatacg gcaatagacg ccacggtgac actccaggac attgggatct cctcgaattt
     3601 gcggctattc ggcaagcgtc tccgtgaaat ctcggggacg tgttttatcg cgatcatatt
     3661 tgggtcgagg gtgcagggat tttgggaact tcgcgtatcc agacgccgtc gatactgcag
     3721 tatcagatag tcgaaaacta aatcagtcgg cgacgagatc ggctcccatc aaagcgaagg
     3781 ccggtacatg acaaggctgc gcgggacttc aagcggcgtt ttgttctgcc agctcgagcg
     3841 tctgttcgcg cagggcaccg caagtggact gacggaaggc gaactgctcg aaagatttgt
     3901 gagtcgacgc gacgaggcgg cctttgaagc cctggtcgcg cgccacggtc cgatggtcct
     3961 gggcgtgtgt cggcacctcc tccgtgaccc caacgatgtc gatgatgcat ttcaggcgac
     4021 tttcctggtg ctggtcaaga aggccagtac cgtccgccgg tgcgagctgt tgggtaactg
     4081 gctgtacggc gttgcttttc gggtggcgtc gcgagcgcgg gcactggcgg cccggcggac
     4141 tgctcgcttt gtctccggag attacgtagt cgaaaacttg acggctgcgg tgtgccgcga
     4201 tgacggcggt ctcgatccga agacgttatt ggaccaaagc ccgtggctgc accaggaggt
     4261 gtgccacttg ccggacaagt atcgcactcc aatcgtgctt tgctacttcg aagggttgac
     4321 gcacgacgaa gcggcgtcgc gtctgggctg gccgctcggc acggtgaagg gacgattg
//
LOCUS       DATAQL010000538         5958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_815530, whole genome shotgun sequence.
ACCESSION   DATAQL010000538 DATAQL010000000
VERSION     DATAQL010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815530"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1096..2334
                     /locus_tag="VHS97_09260"
     CDS             1096..2334
                     /locus_tag="VHS97_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HEX3448428.1"
                     /translation="MEAVGQVTGLPERHLDSWLMPPALCRGLFARAARSAELAVVEGT
                     LDEPISPRMFTSCDCPGDLRPIAEALDLPIVAVVSCPDSHSENFHLPRLPDGVDAVFL
                     DNLGNPAALPCLKRMIRLAANLPVIGAIESMPEARKVLETVPRDRLLPEELIQSLSEG
                     FWKHADLEAIKEIAGRRPFPEMADLSCLRGRDRRCRIFRVAYAQDEAFGRYFPDTLEA
                     LEALGAELIEFSPLRDERLPDGVDLAMIGCGYPDNYADLLVSNVSMIAALREHVCRGR
                     RIYTEGGGTAYLGRRLLIEGRRLPGAGILPFDAEVLPDPKPAAPVTRVLLHDCWLGPA
                     GTVVRAYKSGRWSLIPSVEPFECPASSGPLSAEGDWFYHHHAVGSLLHLHLGALPEVV
                     DAFVGPHSPSLWRPSPRGEA"
     gene            complement(2482..3156)
                     /locus_tag="VHS97_09265"
     CDS             complement(2482..3156)
                     /locus_tag="VHS97_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448429.1"
                     /translation="MSEAQTGSLLVDYFKEFVRTRDVDEFRERVRARYNEGTLGRILT
                     GSPDVSARRAAVLSLGMLGTFEQSNAGLGKALRDQDQAVRSMAEDALWAIWFRADTPE
                     HNQRLEQVRHLIGREQFEQAETLVKRLIVDAPNFAEAYNQRAFILFLLGRFAESAEEC
                     ERVLARNPYHIGAIEGLAKCQLSLNRPRDALKSLRRAFRLRPHNTALGDSIHELEAQI
                     ESDGGR"
     gene            complement(3441..4601)
                     /locus_tag="VHS97_09270"
     CDS             complement(3441..4601)
                     /locus_tag="VHS97_09270"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX3448430.1"
                     /translation="MPSSAPTAMGLNIPLLDRLRAADGDYVPLRELGPDLERVRFDLD
                     ALLSFGFGIEQHPYLGAAYVGPAQRLCPDQIEHGLLTGGIGRRIAVWNRVTSTNDLAM
                     RAGTSSSNDGLVIFAEEQTAGRGRRGRSWMAPPFSSILMSVVLFPPAHPACALPEAGY
                     GRPWLTALGAVAAAEVVTAWTGRHATIKWPNDVRIGGRKIAGILVERAFAPGRPGTAS
                     PARGPAAAEWGAVIGIGLNVNLNRDDLPAELISIATSLEIEGCGSPIDRSEVARHLIR
                     RLDHWYQASRCNGVEVLNLPWRTRSEHLGRFVRITTSAGAMSGRLVEIDLRTGLTMSV
                     ADDAPRTSSEQGMPQLVRLSMGDVLTLEEVSIGLSEHDPSDVGGVGVTMPAS"
     gene            complement(4621..5580)
                     /gene="nadC"
                     /locus_tag="VHS97_09275"
     CDS             complement(4621..5580)
                     /gene="nadC"
                     /locus_tag="VHS97_09275"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX3448431.1"
                     /translation="MTEARYGFGERERHAARALVDQAIAEDLSQIGDITSAATIPSHA
                     RGAARVVARAPGVLAGLAVVDRLVAEFELADYWEPLLHDGDSLVPESLIARIAGPMRS
                     LLAIERTLLNFLQRLSGIATLTARFVAAVSGTQASIYDTRKTTAGWRALEKYAVRCGG
                     GFNHRFGLYDAVLIKDNHLAWLEAAGALGQRDTIATAIATARANTPLGTTIEIEVDTL
                     EQLNRALHGGPDIILVDNLAPELVAEAVRRRDQVAPRVKLEASGGVNLATVRALAQTG
                     VDRISVGALTHSSPALDLALDFDRIPVFHQGTNGAKAVPSAES"
     gene            complement(5685..>5958)
                     /locus_tag="VHS97_09280"
     CDS             complement(5685..>5958)
                     /locus_tag="VHS97_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448432.1"
                     /translation="TFVAGCYTSDANMHDTSMQMAVAAAVDFTPVRLLRKAIATAASL
                     AQGKVISSPAELTVAVEDRPGHAASLRAAVANAKKTADEKSGTAKE"
BASE COUNT         1125 a         1874 c         1790 g         1169 t
ORIGIN      
        1 tcggttgtga cagtgggctc gaaaaccttg agccgttcaa gcgtttcggc tcgaagggcg
       61 gccgcgtcgg catcaagggg accgaccacc gagtttcgag acaccgtggg agccgtcgtg
      121 gagggcggtt gcgagcggga ggagtcgccg cgcgtgtcgg ggtcttggcc tcgcgcggtg
      181 gtccccaaaa tcaacaagag cgagccaagc acgaagtgac gaccggtttt tacatcccgg
      241 ctgccatgtc gagccgaaat ccgagcggtc cggttggacg tcgattgggt tggctgattc
      301 gtcgatcttg gcatcgagcg ggctacctct cggcaagaca ttgagcggct cagtctcgcg
      361 tacggcgcga gcggcgtaaa gtatcccgat gcacccaatg cggcaagtgg aaatgacaca
      421 gaggcgcggc gcacctagaa tctcgtttcg gttgatggag gaaacgctcg actaagcacc
      481 agagtgcgac tgttcgcggt cctaaataca gtttttcatt aacattacgc gacggaaccg
      541 ataaacgagc gagtcgattt cggttgtggc tgacgggatc agtccacgac gaattcactt
      601 tgcgggcagc ggatcggcca aaggggcttg gacactcttg acgcgactta ttaatgcgct
      661 atcatttggt acgaatgggt tgccacgctt gacccggcaa acgaccattt tcatctgctc
      721 gtttctcgcg gaccttgcat gtgaccatcg tcgaatagtg cttgcatggt cctcgtagac
      781 ctcacaacga cggccgacca gacggtcggt ttggcaatcg taaagcggcg actgcttgct
      841 tgaccgaact tgtttggctg acaggttttg cagatggctt tgtgacgggt tgcctgagcg
      901 gcaaaccaga ttggtcggca gcatgttatt gaaagcgcga aatttggtaa gcgtatgatc
      961 cagtttccgc gtttcgcttt ggcgacggtc gcgaagggac ccgaaccgtc ggtggcatcg
     1021 ctggcgatgc tagccggttt gacccatcgc ggctggcgtg ttcaacattt tcgcacacgt
     1081 gcgtgtccga cggcgatgga ggccgtgggg caagtcacag gtctgccgga acggcacctt
     1141 gactcttggc tgatgccccc ggcactttgt cggggactgt ttgctcgagc ggcgcgatca
     1201 gccgagctgg cggtggtaga aggtacgctc gacgagccca tttctcctcg aatgttcacc
     1261 tcgtgcgact gcccaggcga cttgaggccc atcgcagaag cgctcgatct gccgatcgtc
     1321 gcggtggtct cgtgccctga cagccattcg gagaacttcc acttgccgcg gctgcccgac
     1381 ggggtcgacg ccgtcttcct ggacaatctg ggcaatcccg cggcattgcc ttgtctcaag
     1441 cgcatgattc gccttgccgc caacttaccc gtgatcggtg cgattgaatc gatgccagag
     1501 gctcgaaaag ttcttgaaac agttcctcgc gaccgccttt tgccggaaga attgatccag
     1561 tcgttgtctg aaggtttctg gaagcatgcg gacctcgaag caatcaaaga aatagcgggc
     1621 cggcgcccgt ttcccgagat ggcggatttg tcatgccttc gtggccgcga tcgccgttgc
     1681 cggattttcc gcgtggctta cgcgcaggat gaagcgttcg gccgctattt tcctgacacc
     1741 ctcgaggcac ttgaggcgtt gggagcggaa ttgatcgagt tttcgccgtt gcgtgacgag
     1801 cggctgcccg atggggtcga cctggcaatg atcgggtgcg gctacccgga caactacgcc
     1861 gatctcttgg tttccaacgt gagcatgatc gccgcgctcc gcgagcacgt ctgccgcggg
     1921 cgacggattt ataccgaagg aggcggaacc gcctacctgg gccggcgtct gctcatcgaa
     1981 ggccgccgcc ttccaggcgc cggcatcttg ccgttcgacg cagaggtctt gcccgatccg
     2041 aaacccgcgg ctcctgtcac acgtgtcctg ttgcacgatt gctggctggg gcccgccgga
     2101 actgtcgtcc gggcctacaa gagcggccgc tggagcttga tccccagcgt cgagccattc
     2161 gagtgcccgg caagttccgg tcccctctcg gccgagggag attggtttta ccaccatcac
     2221 gcggtcggca gccttctcca tcttcatctg ggtgctctcc cggaggtcgt tgacgcgttc
     2281 gtcggccctc actccccgtc actttggcgt ccatcgccgc gcggagaggc ctagtgaacg
     2341 ctcggaacgg aacgaacgcc cgaatgcgcg acgaagaagt cgacacaaac cacgccttac
     2401 tggccgaaaa cacgatcgat cgccaacgtt ggcccgcctc gagggtggtg cacctcgcct
     2461 aatctcgccc tgctcgtcgc gtcaccggcc accgtccgac tcgatctgcg cttcgagctc
     2521 gtgaatgctg tcgccgagcg ccgtattgtg gggcctgagc ctgaacgccc gtcgcaatga
     2581 cttgagggcg tcgcgaggcc ggttcaaact gagctgacac tttgccaacc cctcgatagc
     2641 gccaatgtga tagggattgc gggcgagaac gcgctcacac tcctcggcgc tctcggcgaa
     2701 tcgacccaga agaaaaagga tgaacgccct ctgattgtaa gcttcggcga agttgggagc
     2761 gtccactatc aaccgcttga ccaatgtctc ggcttgctcg aattgctctc ggccaatcaa
     2821 gtgcctcacc tgctcgagcc tctggttgtg ctcgggcgta tcggcccgaa accagatcgc
     2881 ccagagcgcg tcttcggcca tgcttcgcac agcctggtct tgatcacgca gagctttacc
     2941 caagcccgcg ttactctgct cgaaagttcc gagcataccc agagaaagga ccgcggcccg
     3001 ccgagccgat acgtcggggg aacccgtcag gatccggccc aacgtgccct cgttatagcg
     3061 cgccctcacg cgctcccgaa actcatcgac atctcgtgtg cggacaaact ctttgaaata
     3121 gtcgaccagg agggatccgg tttgggcttc cgacatgctg gaggctccca ttcccgcacg
     3181 aggtgtctcg gaacttgccg aaactccagc ttgggattcc aacggaatcg acacggccag
     3241 cgccaagagc gccagtttca gttttcttgc cagccctcgc gcatcatgcg cgactgcgaa
     3301 attgaagtca gcggataaag gcaagcaagg tcgggatcct gccattgaga tctctcccgc
     3361 gacaacgcct tgcataattg gggaacgtaa gtgtaacagc agtcatgatc cggttccatg
     3421 aaaatccgct tgccaagtta tcatgatgcc ggcatggtta ctcccacgcc gccgacgtct
     3481 gatgggtcgt gttcgctcag accgatcgat acctcttcga gtgtcagaac gtcgcccatc
     3541 gacagccgga ccagctgggg catgccttgt tcgctcgaag tgcgcggagc gtcgtcggca
     3601 acgctcatgg tcaaacccgt tcgcaggtca atctcgacca agcggccact catggcgccg
     3661 gcgcttgttg tgatccggac gaatcgaccc aaatgctcgc tccgagttcg ccatggcagg
     3721 ttcaagacct cgactccgtt gcaccgactc gcttgatacc aatgatccag gcgcctgatc
     3781 aagtgacgcg cgacctccga gcgatcgatg ggcgagccgc atccttcgat ctcgagtgat
     3841 gtcgcgatag agatcaactc agcgggcaag tcgtcacgat tcaggttgac gttcaggccg
     3901 ataccgatca cggcgcccca ctccgccgca gccggtccgc gtgcggggct cgccgtgccg
     3961 ggccgtcccg gggcaaaggc cctttcgacc aggatgccgg ctatcttccg cccgccgatc
     4021 cgaacatcgt tgggccactt gatcgtggcg tggcggcctg tccacgcggt gaccacctcg
     4081 gctgccgcca ccgcgcccag ggcagtcagc cagggacggc cgtaacccgc ctcgggaagg
     4141 gcacaggccg ggtgcgcagg aggaaacagc accaccgaca tcaagatcga cgaaaagggt
     4201 ggggccatcc aagaccgccc gcgtcgaccc cggccggcgg tctgctcctc ggcaaagatc
     4261 accaggccat cattcgacga cgacgtccca gcgcgcatcg ccaggtcatt tgtgctcgtg
     4321 actcggttcc acacggcgat tcgccgtcca atcccgcccg taagcagacc atgctcgatc
     4381 tgatcaggac agagccgttg ggccggacca acgtacgcgg cgcctaggta gggatgctgc
     4441 tcgatcccaa agccgaagga caagagcgca tcgaggtcga atcgcacccg ctcgagatcc
     4501 ggaccgagct cgcgcaatgg cacgtaatcg ccatcggccg cgcgcaggcg gtccaagagg
     4561 gggatgttca gacccattgc agtcggcgcg gacgagggca cggtcgtgct ccaattcgcc
     4621 tcaagattca gcgcttggga cggccttcgc cccgtttgtc ccctggtgaa acaccgggat
     4681 cctatcgaag tcgagcgcga gatctaaggc cggcgacgag tgagtgagcg cgccgacgct
     4741 gatgcgatcg actcccgttt gagccagagc ccgcaccgtg gcaagattca cgccaccgga
     4801 agcctcgagc tttacccggg gcgcgacttg atcgcggcgg cgcacggcct cggcaacgag
     4861 ctcaggcgcc agattatcga ccaggataat gtcgggaccg ccatggagcg cccggttgag
     4921 ttgctcgagc gtatcaacct cgatctcaat cgttgtaccc aagggcgtat tcgccctggc
     4981 ggtcgcgatc gcagtcgcga ttgtgtcgcg ctggcccaga gcaccggccg cctcgagcca
     5041 cgcgagatga ttgtccttga tgagcacagc atcatataaa ccaaaacggt ggttaaaccc
     5101 tcctccacat cggactgcgt atttttccag ggcgcgccaa cccgcggtgg tcttgcgcgt
     5161 gtcgtaaatc gaggcttgcg tgccggagac cgcggcgaca aaccgtgccg tcaaggttgc
     5221 gatcccgctc aaccgctgga ggaagttgag cagcgtccgc tctatcgcca gcaacgaacg
     5281 catcggcccc gcgatccggg cgatcaggct ctcgggcacg agcgaatctc cgtcgtgaag
     5341 cagcggctcc cagtaatcgg cgagctcgaa ctccgcgacc aatcggtcga cgaccgccag
     5401 gccggccaga actccagggg cccgtgccac cacccgcgcg gcaccgcggg cgtggcttgg
     5461 aatcgtcgcg gccgaggtga tatcgccgat ctgactcaga tcttccgcta tcgcttgatc
     5521 gacgagcgcc cgggcggcgt gccgttccct ctcaccgaat ccataccgag cctcggtcat
     5581 gaaaacgacc tcctccgtac ttgcacgggc ttccagcccg tgtctgaaaa cgccacgggc
     5641 aagatgcccg tgccacacaa gcccgccgag cgcgtttgct caatctattc tttcgcggtt
     5701 cctgactttt cgtccgctgt tttttttgcg ttggcaaccg ccgctcgtag agacgccgca
     5761 tgcccaggcc tatcctccac ggcaaccgtc aactcggctg ggctcgatat aactttgccc
     5821 tgtgccaggc tggcggccgt agcgatcgcc ttgcgaagca gtcgcaccgg cgtgaagtcg
     5881 accgcggccg ccacggccat ttgcatgctg gtgtcgtgca tgttggcatc gctggtatag
     5941 catcccgcaa cgaacgtc
//
LOCUS       DATAQL010000539         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1428109, whole genome shotgun sequence.
ACCESSION   DATAQL010000539 DATAQL010000000
VERSION     DATAQL010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428109"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VHS97_09285"
     CDS             complement(<1..397)
                     /locus_tag="VHS97_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3448433.1"
                     /translation="MTIFRHATIASLVISSATIALSQAEQPRASNSSSSDWRPLFDGR
                     TLDGWEHVGPGKFVVEDGVLRTEGGMGLLWYAREKLGNCVIRVVYKTANVRSNSGVYL
                     RIADKPKDAWYAVHHGFEIQVMDQSTSGRG"
     gene            complement(478..1167)
                     /locus_tag="VHS97_09290"
     CDS             complement(478..1167)
                     /locus_tag="VHS97_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3448434.1"
                     /translation="MSDQPATAHDAPILRADGLAKYYPDGHVQALRGVSLQIEPSESV
                     AITGPSGCGKSTLLHLLGGLDRPTQGDVFFREEPLSKLDIDAFRAHQVGFVFQSFYLM
                     PTLTALENIQIPMFEGPWPPRQRGERALRLIEEVGLADRRDHRPGQLSVGQRQRIAIA
                     RALANDPQLLLADEPTGNLDSKSQDEIIHLLHRLRDERRLTLVIVTHSHDVARAADRR
                     IPMKDGQIQPE"
     gene            complement(1160..2284)
                     /locus_tag="VHS97_09295"
     CDS             complement(1160..2284)
                     /locus_tag="VHS97_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3448435.1"
                     /translation="MRFISLIVKNVLHRPVRTLLTTLGLAIAIAAVTILVGISWNFER
                     SFLAIYNSKGIDLVVVHAGSSNQLSSSLDEKLADRLRQVPGVAGVAPSLVDTVGFEEQ
                     NLVSVLVNGWVPGSILFRGLRVLEGRALEPGDDKAVMLGRVLTMTLGQKAGDRLQIAG
                     ETFDVVGIFESDSWFENGGLLMPLKALQKMMGREGQVTGFLIQAAKSDERSIAELRKR
                     IESTISGVAATPARDHVQGDTQIRLSRAMAWTTAAVALFLGSVGMLNTMLMSIFERTR
                     EIGILRAVGWRRRRVLRLIMGEALAIALMGTVLGTLLAVVGLRAITLAPTARGFINPD
                     LPPQVLGIALAMGIGLSLLGGLYPAARAAALEPTEALRYE"
     gene            complement(2321..3175)
                     /locus_tag="VHS97_09300"
     CDS             complement(2321..3175)
                     /locus_tag="VHS97_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3448436.1"
                     /translation="MSTISPRQVTTSHWQTDWDIWRCLDCQGELRYDPATSVMQCSQC
                     AARLAINDDILIVTDRTTANNQVAQQFYDSPLWPRFRFWEWFTWVCNGGERRARNKVL
                     RHLPTTPGLDLLDVAVGDGVYLDWLPADWRIVGVDISRSQLEACRRRSAGRSVWLAQG
                     EAEDLPLESQSFDAVLSIGAFNYFNDPEGALKEMIRVARPGAPIVVSDEVPNLTDRML
                     GHKLGFPGVDRWLVSRMMHLGDSFTDMVERHRDLNIAKIAGRVFKEFQLESVWLGVGY
                     VLVGRAPE"
     gene            complement(3201..>3769)
                     /locus_tag="VHS97_09305"
     CDS             complement(3201..>3769)
                     /locus_tag="VHS97_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3448437.1"
                     /translation="MEILHHVFGVDTATARVLEVGIGSGENLTFLRRGWNVFGIDIAR
                     TQLEACLRRHPAMAGHLAWAEAERLPFADATFDASWSVGGFNYFSDHERALREMRRVT
                     KPGSPVVVADEVPGLHRAGLGHLLGIPSLDAWWLHKLGLDREFVRMVLEFNVDLTGLA
                     NRVWPHHTRHRIWHGMGYCLVDKSGLSE"
BASE COUNT          771 a         1166 c         1172 g          660 t
ORIGIN      
        1 tgccgcgacc gctggtcgat tggtccatga cctgaatctc gaaaccgtga tggaccgcgt
       61 accatgcgtc cttgggcttg tcggcgatac gaagatacac gccagaattg gagcggacat
      121 tggccgtctt gtagacgacc cgaatcacgc agttgccgag cttctcacgt gcataccaca
      181 agagccccat tcctccctcg gtacggagca caccgtcttc gacgacaaac ttgcctggcc
      241 ccacatgttc ccacccatcg agggtgcggc cgtcgaagag gggtcgccaa tctgaggacg
      301 aggagttgga tgcgcggggc tgctcggcct gagagagggc gatcgttgcg gaagagatta
      361 caagcgacgc gatggttgca tgacggaaaa ttgtcatcgt gtattcctgg cctgcgtggt
      421 cgtgggttac ctgtctcaaa actaacgcgc caacggtcgc cgatcaaccc gaccacttca
      481 ctcaggctga atctgcccgt ccttcatcgg aattcgccgg tcggcggcgc gggcgacgtc
      541 gtggctgtgc gtgacgatca cgagcgtgag ccgacgctcg tcgcgcaggc gatgcagcaa
      601 gtggataatc tcatcctggc tcttgctatc gagattaccg gtcggctcgt cggcgaggag
      661 cagttgaggg tcattggcga gagctcgcgc gatcgcgatg cgctggcgct ggccgaccga
      721 gagctggccg gggcggtggt cgcgcctgtc cgcaaggccg acttcctcga tcaaccgcag
      781 ggcgcgctcg ccgcgctggc gcggcggcca agggccctca aacatgggga tctgaatatt
      841 ttcaagcgcg gtaagggtcg gcatgagata aaaggattga aatacgaatc ccacctggtg
      901 agcacgaaag gcgtcgatat cgagcttcga cagcggttct tcccggaaaa agacgtcgcc
      961 ctgagtgggc cgatcgagcc cgccgagcaa atgcaggagg gtgctcttgc cgcaaccgct
     1021 gggcccggta atcgccaccg actcggatgg ttcgatttga agcgagacgc cgcggagtgc
     1081 ctgaacatgg ccgtcggggt agtacttcgc gagcccgtcg gcgcggagga tgggggcatc
     1141 gtgcgccgtc gccggttggt cactcatagc ggagcgcctc ggtcggttcc agagcggccg
     1201 ctcgagccgc cgggtaaagg cccccaagca aactgaggcc gatgcccatc gccagcgcga
     1261 tacctaaaac ctggggcggc aggtcgggat tgataaaacc tcgcgccgtg ggagcgagcg
     1321 ttatcgcgcg gaggccgacg accgcgagca gggttcccag cacagttccc atcaaggcga
     1381 tcgccagcgc ttcacccatg atcagacgca gcacgcgccg gcgcctccag ccgacggcgc
     1441 gtaaaatccc gatctcgcgc gtgcgttcaa agatcgacat gagcatcgta ttgagcatcc
     1501 cgactgagcc caggaagagg gcgaccgcgg ccgtggtcca ggccatcgcc cgggagagac
     1561 ggatctgggt gtcgccttgc acatgatcgc gcgcaggggt cgcggccact ccggaaatgg
     1621 ttgactcgat acgcttgcgc aattcggcga tcgaccgttc gtcagacttt gccgcctgga
     1681 tcaaaaaacc ggtcacttgt ccctcccgac ccatcatttt ctggagggcc tttagcggca
     1741 tcagcagtcc gccgttttca aaccagctat cgctttcgaa gatccccacg acgtcgaacg
     1801 tctcgccggc aatctggagg cggtcgccgg ctttctggcc gagcgtcatc gtaaggactc
     1861 ggccgagcat gaccgcttta tcgtcgccag gctcgagcgc ccggccttcg agaacgcgaa
     1921 ggccccgaaa taaaatgctg ccgggaaccc agccattgac gagcacactc accaggttct
     1981 gttcttcaaa accgactgtg tctaccagcg acggggccac gccggccact cccggaacct
     2041 gccgcagacg atctgccagt ttttcgtcca ggctgctcga aagctggttg gagctgcccg
     2101 cgtggacgac caccaggtcg atccccttgg agttgtagat cgccagaaac gaccgttcaa
     2161 aattccatga gatccccacg aggatcgtca ccgcggcgat ggcgatcgcc aggcccaggg
     2221 tcgtcagcag cgtgcggaca gggcggtgga gaacgttttt gacaatcagt gaaatgaacc
     2281 gcacggatcg gtcctcacgc gattgtgatc ccgaccagac ttactcggga gcccgtccga
     2341 ccaggacgta tccgacaccc aaccacaccg actcaagctg gaattccttg aagacgcgcc
     2401 cggcaatctt ggcgatgttc aggtcgcggt gacgttccac catatcggtg aatgagtctc
     2461 ccagatgcat catgcgcgag accagccagc gatcaacgcc tggaaaccca agtttgtggc
     2521 ccagcatccg gtcggtgagg ttgggcactt catcggacac gacgatagga gcgcccgggc
     2581 gtgcgacgcg gatcatttcc ttgagcgctc cctcgggatc gttaaagtaa ttgaaggccc
     2641 cgatagagag gacggcgtcg aagctctgac tctcgagcgg gagatcttcc gcctctccct
     2701 gagcgagcca gacggatcgc ccggcggagc ggcggcggca ggcctcgagc tgagaacgcg
     2761 agatgtcgac gcccacgatt cgccagtcgg caggcagcca gtcgagatag acgccatcac
     2821 cgacggccac gtcgagcagg tccagtcccg gggtggtcgg gagatgccgc agcaccttgt
     2881 tgcgagcgcg gcgctcgccg ccattgcaga cccaggtaaa ccactcccag aaacgaaacc
     2941 tcggccagag agggctatcg tagaattgct gggcaacctg gttgttcgcc gtcgtccggt
     3001 ccgtgacgat caagatgtcg tcgttgatcg ccaggcgagc cgcgcattgc gagcactgca
     3061 tgaccgacgt tgccggatcg tatcgcaact cgccctggca atcgaggcac cgccagatgt
     3121 cccagtcagt ttgccagtgt gaggttgtga cctgccgggg gctgatggtc gacatggaga
     3181 gactcccaaa ttggatcaga ttattccgac aagccggact tgtcgacaag acaataaccc
     3241 atcccgtgcc agatgcggtg ccgtgtatgg tgcggccaca cacggttggc aagaccggtg
     3301 agatcgacat tgaattcgag gaccatcctg acgaactcac ggtcgagccc gagcttgtgc
     3361 agccaccagg cgtcgagtga cggaatgccc aacaaatggc ccaggccggc gcgatgaagc
     3421 ccggggacct catcggcgac caccacgggg ctgcccggct tggtcacgcg gcgcatctcg
     3481 cgcagagccc gctcgtggtc gctgaaatag ttgaacccgc cgaccgacca ggacgcgtca
     3541 aacgtggcat cggcgaaagg aaggcgctcg gcctcggccc aggccaagtg tcctgccata
     3601 gcgggatgcc ggcgcaggca cgcctcaagc tgggtccgcg cgatgtcgat tccaaaaacg
     3661 ttccatccac ggcgcagaaa ggtcagattt tctccgctac cgatcccgac ctcgagcaca
     3721 cgagcggtgg ccgtatcaac tccgaagaca tggtgcaaga tctccatac
//
LOCUS       DATAQL010000540         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3871758, whole genome shotgun sequence.
ACCESSION   DATAQL010000540 DATAQL010000000
VERSION     DATAQL010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3871758"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VHS97_09310"
     CDS             complement(<1..187)
                     /locus_tag="VHS97_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448438.1"
                     /translation="MRSRTVLFTLALNLLAMPPLAAALSAVRSDAEAQSQRSLLRMDF
                     GKTPEGAPVELYVLTNGK"
     gene            complement(392..1669)
                     /locus_tag="VHS97_09315"
     CDS             complement(392..1669)
                     /locus_tag="VHS97_09315"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3448439.1"
                     /translation="MSTADRSRHVVILGAGFAGLSAARGLRSSPVRISIIDRSNHHLF
                     QPLLYQVATAALSPADIAAPIRRIFRHQSNVSVMLAEATAINLDQKRVVLADGAVDFD
                     ILIVATGATHAYFGHDDWGEYAPGLKSLHDALRIRQRVLMAFEVAERETDDSRRRAWM
                     TFVIVGAGPTGVELAGTLAEVSRQTLARDFRHINTASARVILVEATPRVLGAYTQDLS
                     EAARRQLEKLGVTVWTGVQVTGIDAEGVWIGRERVHAHTVVWAAGVAASPLGVTLGVP
                     LDRAGRVLVEPELTIPGRDDVYVIGDLAHVEKDGALVPGVAPAAMQEGWHVAGNITRT
                     LRGQPRLPFHYVDKGMLATIGRGAAVAKIGPIKASGHFAWLLWLFVHILFLIGFRNRL
                     LVMFQWAWSYVTFDRGARLITEPLKEPLVASHS"
     gene            complement(1692..3062)
                     /gene="zwf"
                     /locus_tag="VHS97_09320"
     CDS             complement(1692..3062)
                     /gene="zwf"
                     /locus_tag="VHS97_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3448440.1"
                     /translation="MNQNHSDALVFFGATGDLAYKKIFPALQHLIQHGHLDVPVIGVA
                     KAGWTLEQLQARARDSIEKHGEMDEKAFAELVKLLRYVDGDYNDLETFAQLRQAFDGA
                     RRPLHYLAIPPSMFSIVVENLAKTGCADNARVVIEKPFGHDEESARELNGILLKTFDE
                     ARIFRIDHYLGKEPVLNILSFRFANQFLEPIWNRNCVESVQITMAESFGVQGRGAFYD
                     EAGTIRDVVQNHMLQVVAMLAMEPPGSNSADGIRDEKVKVLRAIRPLDPASVVRGQYV
                     GYREEKGVAANSEVETFVALRLEIDSWRWTDVPFLIRSGKCLAKTSTEVMVRLHSPPQ
                     RCMATQSLDHTHNYVRIRFNPEEIIAIGAVIRKEGEKEDLLPVELLVSRQAADEVPPY
                     ARLLQSAMIGDPSLFAREDLVEAQWSIVEPVLDNTTPLLFYKPGSWGPPEADRLLASG
                     DEWHNP"
     gene            complement(3087..>3750)
                     /locus_tag="VHS97_09325"
     CDS             complement(3087..>3750)
                     /locus_tag="VHS97_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3448441.1"
                     /translation="NKGLLPPDYYALPEQQAAGFGPDVLTLQGQPDQRGDESLETGRS
                     LTLLSRPRTRFMAESDAEFYRRKKSSVVVRHVSGDRIVSMVEIVSPGNKSSRHAFRAF
                     VDKACELLEARVHLLIVDPFPPGPRDPHGVHAAIWEEVRDEPFHLPDDKQLTLVAYEC
                     GLTTRAYIEPVAVGDSLPEMPLFLEPDGYVKVPLDTTYQTAFNVLPSRWRNVLEPPDS
                     RR"
BASE COUNT          758 a         1140 c         1144 g          708 t
ORIGIN      
        1 ttttgccatt cgtgagcacg tacaactcga ccggagctcc ctccggcgtt tttccgaagt
       61 ccatcctaag aaggctcctc tggctctggg cttccgcgtc ggatcgcact gcgcttaatg
      121 cggcggccaa aggcggcatc gccagcagat tgagggcgag cgtaaacaac acggtacggc
      181 tgcgcatgga tcggcctctc ttgttgaata acagaggata ccgcagcgct ccgcgaatag
      241 tcagtagcat gtcggcaaaa gtgcgatccc ggccacccat gccggattgt aacgcgccat
      301 ttcgggccag agcgcattca agggcggcta cgagacaaag gggtaaggac cattttctac
      361 aaaaaaaggt tcgtgacgcg tttgtctctt tttaactgtg cgacgcgacg agcggctcct
      421 tgagcggctc ggtgatcaac cgtgcgccac gatcgaaggt gacgtacgac caggcccact
      481 ggaacatgac caaaagacgg ttgcgaaacc cgatcaggaa caggatatgg acgaacaacc
      541 agagcagcca ggcgaaatgg cccgacgcct tgatcggccc gattttggcg acggcggcac
      601 cgcggccgat ggttgccagc atccctttgt cgacgtaatg aaacggcagg cgaggctggc
      661 cgcgcagcgt tcgcgtgatg ttgccagcga cgtgccagcc ttcttgcatc gcagcggggg
      721 cgacccccgg caccaaagcg ccgtcttttt cgacgtgagc cagatcgcct ataacgtaga
      781 cgtcgtcgcg tcccgggatc gtcagctctg gctcgaccaa gacgcgacca gcccgatcga
      841 gtggcacacc gagagtcact cccagcggcg aggccgctac ccccgcggcc cagaccacgg
      901 tgtgggcgtg gacccgctcg cggccgatcc agaccccctc ggcgtcgatt cccgtgacct
      961 ggaccccggt ccagaccgtc acgccaagct tttcgagttg tcggcgagcc gcctccgaga
     1021 ggtcctgggt ataagcgccc aagacccgcg gggtggcctc gaccaggatg acccgggccg
     1081 acgccgtgtt aatgtggcgg aagtcgcgcg cgagggtctg ccgggaaact tcggccagcg
     1141 tgccggccag ctcgacgccc gtcgggccgg cgcccacgat cacaaacgtc atccaggccc
     1201 gtcggcggga gtcgtcggtc tcgcgctcgg cgacctcgaa agccatgaga acacgctgcc
     1261 ggatccgtaa cgcatcgtgg agtgatttca gcccgggggc gtactcgccc cagtcgtcgt
     1321 ggccgaaata agcgtgggtc gcgcccgtgg ccacgatcag aatgtcgaaa tcgaccgcgc
     1381 cgtcggcaag cacgacccgt ttctggtcaa gattgatggc cgtggcttcc gccagcatca
     1441 ccgacacatt cgactgatgg cggaaaatcc ggcggatcgg cgcggcgatg tccgccggac
     1501 tgagcgcggc ggtcgccact tggtagagga gcggctgaaa gagatggtgg ttggagcgat
     1561 cgataatcga tatgcgcacc ggagaagagc gcaagccacg cgcggccgac agaccggcga
     1621 aaccagcgcc caggatgacg acatggcggg atcgatcggc cgtgctcatg gaatcatcca
     1681 tgtagatccg ttcaaggatt gtgccactcg tcaccgctcg caagcaaacg gtccgcctcg
     1741 ggcggtcccc agcttcccgg cttatagaaa agaagaggtg tcgtgttgtc aagtaccggc
     1801 tcgacaatgc tccactgcgc ttcgaccaag tcctcgcgcg cgaaaaggct cggatctcca
     1861 atcatggccg attgcaagag gcgcgcgtag ggaggaactt cgtcggccgc ttgccggctg
     1921 accaatagct cgaccggcag cagatcttcc ttctccccct ccttacgtat caccgcgccg
     1981 attgcgatga tctcctcagg attgaagcgg atgcgaacat aattatgcgt atgatcgagc
     2041 gactgcgtag ccatgcatcg ctgggggggc gagtgcaagc ggaccatgac ctcggtggat
     2101 gtcttggcca ggcacttgcc ggatcgaatc aagaacggca catcagtcca gcgccacgag
     2161 tcgatttcca ggcggagcgc gacgaaggtc tcgacttctg agtttgcggc gacccctttt
     2221 tcctcacggt agcccacata ctgaccgcgg acgacgctgg ctggatccag cgggcggatc
     2281 gcccgcaaca ccttgacttt ctcgtcgcga attccatcgg cagaattgct gccggggggt
     2341 tccatcgcga gcattgcgac gacctggagc atgtggttct ggaccacatc gcggatcgtg
     2401 ccagcctcgt cgtagaaggc gccgcgaccc tgcacgccaa acgattcggc cattgtgatc
     2461 tgcacgctct cgacgcaatt gcggttccag atcggttcca agaattgatt ggcgaaccgg
     2521 aacgagagaa tgttcaagac cggttccttg cccagataat ggtcgatccg aaagatccgc
     2581 gcttcgtcga acgtttttaa gagtatgcca ttcagctcac gggcagactc ttcgtcgtga
     2641 ccgaatggct tttcgatcac gacacgcgcg ttgtcggcgc atccggtctt cgccaggttt
     2701 tccaccacga tgctgaacat actcggcgga atcgccaggt aatgcagcgg gcgccgcgcg
     2761 ccatcgaagg cctggcgtaa ctgagcgaag gtctcaagat cgttgtaatc cccgtcgacg
     2821 taccgcagta gcttgacgag ctcggcaaac gctttctcat ccatctcgcc gtgcttttcg
     2881 atgctgtctc gcgcccgggc ctgtaactgc tcgagcgtcc agccggcctt ggccacgccg
     2941 atgacgggaa cgtccaagtg tccgtgctgg atcaaatgct gcagtgcggg gaagatcttc
     3001 ttgtaggcca ggtcgcctgt cgccccgaaa aacaccaggg catcggaatg gttctggttc
     3061 aaggtggtgt ctccggcatg tcggtttcac cttcgcgagt cgggaggttc gagcacattt
     3121 cgccaacgcg aaggaagaac gttgaaagcc gtctggtaag tggtatccag gggaaccttc
     3181 acgtatccgt ccggttctag aaagagaggc atttccggaa gagagtcacc aacagccacg
     3241 ggctcgatgt aagcacgagt ggtcagcccg cactcgtagg cgaccagagt cagttgttta
     3301 tcgtcgggaa ggtggaaggg ttcgtcgcgt acctcctccc agatcgccgc atgaacgcca
     3361 tgggggtcac gcggtcctgg cgggaacggg tccacaatga gaaggtgaac tcgggcctca
     3421 agcaactcgc atgccttgtc cacgaaggcc cggaacgcgt ggcgactgga tttgttccca
     3481 ggcgagacga tctcgaccat cgatacgatc cggtctccac tgacgtgccg gacaaccacc
     3541 gaactcttct tccggcggta gaactcggca tccgactcag ccatgaaccg ggttcgtggc
     3601 cttgacagca acgtgaggga ccttccggtt tcaagggatt cgtcgcctcg ctggtccggt
     3661 tgaccctgca atgtcaaaac gtccggcccg aaacccgcgg cttgctgctc gggcagtgcg
     3721 tagtaatcgg gcggtagcaa tcccttgttg
//
LOCUS       DATAQL010000541         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_512847, whole genome shotgun sequence.
ACCESSION   DATAQL010000541 DATAQL010000000
VERSION     DATAQL010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512847"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2129)
                     /locus_tag="VHS97_09330"
     CDS             complement(<1..2129)
                     /locus_tag="VHS97_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX3448442.1"
                     /translation="MQPSQARDSAIKAITSWPTSGRRAPRTASPIRTLFGTNVFSDEI
                     MKSRLPENVYKAIRNTIKKGAPLDSTIADVVATTMREWAMEHGATHYTHWFQPMTGLT
                     AEKHDSFLVPTEGGGAIAEFSGSELVRGEPDASSFPSGGIRTTFEARGYTAWDPTSPA
                     FILDNPNGTTLCIPTAFCSWTGEALDKKTPLLRSMEALSKQALRILRLFGSEATRVYS
                     TAGPEQEYFLIDKHFYFARPDLINAGRTLFGARPPKGQELEDHYFGAIPERVLACMLE
                     TETELYKLGVPVKTRHNEVSPAQYELAVTFESANVATDHNMLVMETMKRVADRYGLQL
                     LLHEKPFAGVNGSGKHLNWSMAEDTGANLLKPGDTPHDNAQFLLFLLAVIRAVARHGD
                     ILRVSVANAHNDHRLGANEAPPAIISVFLGDMLQDIIEQIEKGGARSAKTGGELKIGV
                     SVLPTLPRDPGDRNRTSPFAFTGNKFEFRALGSSQSVAGSIVALNTIVAESLDDLATH
                     LEQAVSAGKDLNAEIQLLLQNAVKESKHVIFNGDNYSEAWHAEAERRGLPNRRTTVDS
                     LPDLISPKSVKLFSKYGVFSERELHSRYEIFVEAYRKTINIESQLTIHIAKRMILPAA
                     LRYQSEVAHSIANLKASGATVPKIQSAHLSELVSLIDELQSSTYRLEAALEEHPDGDS
                     LAHAKHARDVIIPAMNSVRVAGDNLEG"
     gene            complement(2538..3005)
                     /locus_tag="VHS97_09335"
     CDS             complement(2538..3005)
                     /locus_tag="VHS97_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448443.1"
                     /translation="MNVIRKSTWTILFLGTVIGCSGQEEAVPPPESKPEIPPAGANAA
                     PPVSPAASGESKKGDEAPKIEGPKAENTKPDGGAVVLTVAELAAVKELPESEQAAASQ
                     QGVCPVSTHHLGSMGKPLKVTADGRTFYICCDACEDKVKNDPKSVIAILDKAK"
BASE COUNT          618 a          939 c         1018 g          695 t
ORIGIN      
        1 ataccttcga gattgtcgcc ggcaacacgt acgctgttca tggcgggaat gatcacgtcg
       61 cgggcgtgct tcgcgtgagc caacgaatcg ccatcgggat gctcttcgag cgccgcttcc
      121 agccggtagg tcgatgactg aagctcatcg atcaaggaga cgagctcact caagtgagcc
      181 gactgaatct tgggaacagt cgcacccgag gctttgagat tggcaataga atgggcaact
      241 tcagactggt atcgcagtgc ggcgggcaga atcattcgct tggcgatatg gatcgtcaac
      301 tgcgactcga tgttgatcgt cttgcgatag gcttccacga agatctcgta gcgcgaatga
      361 agctcgcgtt cgctgaagac accatatttc gagaacaact tgaccgactt gggagaaatc
      421 aagtccggta gactgtcgac tgttgtgcgg cggttgggaa gtccgcgccg ctcggcctcg
      481 gcgtgccacg cctcgctgta gttgtcgccg ttgaagatga cgtgcttgct ctctttgacc
      541 gcgttttgga ggaggagctg gatttcggcg ttcaggtcct tgccggcgga gacagcctgt
      601 tccaggtggg tggccagatc gtcaaggctc tccgcgacaa tcgtgttcaa ggccacgatc
      661 gagccggcaa ccgactgact cgatccgagc gctctgaact caaatttgtt acccgtgaac
      721 gcgaacgggc tcgtccggtt acggtcgccg gggtctcggg gcaaggtcgg cagcacggaa
      781 acgccgattt tgagctctcc gccggtcttc gccgagcggg cgcccccttt ttcgatctgt
      841 tcgatgatgt cctgaagcat atcgcccagg aacaccgaaa taatggctgg cggagcctcg
      901 tttgccccca gcctgtggtc gttgtgtgcg ttggcgaccg agacgcgtag gatgtcgccg
      961 tgtcgagcga ccgcgcggat gaccgccagc aagaacagca ggaactgggc gttgtcatgc
     1021 ggggtgtcgc cgggtttgag caggttggct ccggtgtctt cggccattga ccagttcagg
     1081 tgctttcccg agccgttgac gccggcaaac ggcttctcgt gcagcagaag ctgaaggcca
     1141 tagcggtcgg ctactcgctt catcgtctcc ataaccagca tgttatggtc ggtcgccacg
     1201 ttggcgctct cgaaggtcac cgcgagctca tattgagccg gcgagacctc gttgtgccgc
     1261 gttttgaccg gaacgcccaa cttgtagagc tcggtttccg tctccagcat acacgccaac
     1321 acgcgctcgg gaatggctcc gaagtaatgg tcttccagtt cctggccctt gggaggtctc
     1381 gcgccaaaga gggtccggcc ggcattgatc aggtcgggcc gcgcgaagta gaagtgctta
     1441 tcgatcagga aatactcctg ctcgggaccg gccgtgctgt agacgcgggt cgcttccgaa
     1501 ccaaaaagtc gaaggatgcg cagcgcttgt ttcgagagcg cctccatcga gcggagcagg
     1561 ggggtcttct tatcgagcgc ctcgcccgtc cacgagcaaa acgcggtcgg tatgcacagg
     1621 gtggtgccgt tgggattgtc gaggatgaag gcgggactcg tcgggtccca cgcggtgtag
     1681 ccgcgagcct caaatgtggt gcggatccca cccgacggga aactggaagc gtctggttca
     1741 ccgcggacca actccgaccc gctgaactcg gcgatcgccc cgcccccctc cgtgggcacc
     1801 aggaacgaat cgtgtttctc ggccgtcaac ccggtcattg gctggaacca gtgcgtgtaa
     1861 tgcgtcgcac cgtgttccat cgcccactct cgcatcgtgg tggcgaccac gtccgcgatc
     1921 gtcgagtcga ggggagcgcc tttcttgatt gtgttgcgga tagccttgta gacgttttcg
     1981 ggcagacgcg acttcatgat ttcgtcggaa aacacattcg tcccgaacag cgtgcgaatg
     2041 ggtgacgcgg tccggggggc tcgtcgaccg cttgtcggcc aggatgtgat cgccttgatg
     2101 gccgaatcgc gagcttgtga gggttgcatg gcgggtctcc gttaaacact tcgaattcga
     2161 tgggcgtcag agtcgcggtc gcgtcattcg gacccggtcg atgcgcccgg gagtgggact
     2221 gtcggcaacc aagaaattgc cgctatggcg cgccgtgtgg gtggatggcg agatctaaga
     2281 cctacttggg tgaaagatta tatccagcgc aacgccccga cgccagactc gaatcggtcg
     2341 gaagaatgac tccgacctta ttcctggccg ttgcgatagt gaagagactg atgacctcgg
     2401 cacggatccg ttatgaaacg agtctccgag agccatcagt catagagcga taatgcatgc
     2461 cgccacggcc gggaccggcc agcagataca cccagctcag gcgggaagca aaacgataca
     2521 aggcaagacg tttgccgtta ttttgctttg tcaaggatgg cgatgacaga cttgggatcg
     2581 ttcttgacct tgtcttcgca ggcatcgcaa cagatgtaaa atgttcggcc gtccgcggtc
     2641 accttgagag gcttgcccat ggaaccgaga tggtgcgtgc ttaccgggca gacaccttgc
     2701 tggctcgccg ccgcctgttc ggattccggc aattccttga cggcagcaag ctctgcgacc
     2761 gtgagtacca ctgctccgcc gtccggcttg gtgttttcgg ctttgggccc ttcgattttc
     2821 ggagcctcgt cacctttctt cgactctcca gatgcggcag gactcacggg gggagcagca
     2881 ttagcgcccg cgggcggaat ttccggcttc gattcagggg gaggcaccgc ctcttcctgg
     2941 ccgctgcagc cgatgacggt gcccagaaaa agtatagtcc aggtcgattt gcggatcaca
     3001 ttcatcggaa acatctctcg tcgttgatgc ggttggaacc gactcgccgc gacgctcacg
     3061 ttaacgtcga ggattcattc aaaattctaa ccgcgatccg gttaggccgc caaggcggcg
     3121 ctgcacgcga tcgctcggcc cgtgccggtt agtaccggtc agaccggctc gcgatccgac
     3181 tcaagaaggt cgcgctccgc cgccggtata cccttgtaga tccgccggtt atcgagcgcg
     3241 aaaacccgct ccgtgaacga gtcgtcggtc
//
LOCUS       DATAQL010000542         9890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2495548, whole genome shotgun sequence.
ACCESSION   DATAQL010000542 DATAQL010000000
VERSION     DATAQL010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2495548"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            831..2108
                     /locus_tag="VHS97_09340"
     CDS             831..2108
                     /locus_tag="VHS97_09340"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3448444.1"
                     /translation="MNVDRFALVALVVVNLALAPYFVFLLATSLAALASRRARRSSED
                     PRSRFLIVIPAHDEESGIKTTVRSCLAADYPSSLFGVLVIADNCSDRTAYLAAEAGAR
                     VVERFDSLKKSKGYAIEYLMESLELSGEFDAFDALVIIDADTTMDADLLRYFDQGLQE
                     GRDWIQCYYTVANPDQSWRTRLMTYAFSLFNGVMLLGQNRLGASAGFKGNGMCLTTRG
                     LRRRPWRSYGLVEDMEYSWTLRIMGEKIAFAPEVAVHGAMLGSGGAAAANQRRRWEFG
                     RGDIRKKYLGPLLRSNRISWWEKAVSACELTIPSMGVLALLYVIVLVLNTVAWFANGT
                     YLPPVARGFLLASCILMTASMVAYGISPFVVMRLPWRYAWSIALFPVYLIWKLLISLK
                     GRPQQWVRTARESQREGPRNFRDRVSASRKLGE"
     gene            2429..3433
                     /gene="gap"
                     /locus_tag="VHS97_09345"
     CDS             2429..3433
                     /gene="gap"
                     /locus_tag="VHS97_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX3448445.1"
                     /translation="MALRVGINGFGRIGRLVFRVMAERPNDFDVVAINDLSDPKHLAL
                     LLKYDSVQGRFKGTVEARDRSIAVNGKDIPITTEKDPAQLPWKKLGCQIALESTGRFT
                     DRANLQKHIDAGAERVVLSAPAKDDLDATIVLGVNDKILNKSHRIISNASCTTNCLAP
                     LAKVLNEAFGIESGLMTTVHAYTNDQRVADQIHEDPYRARAAAVNIIPAKTGAAKAVG
                     EVIPELKGKLTGSSLRVPVPAGSVVDLTVLLKTSASRDQINGALKAAADGPLKGILEY
                     ATDPIVSSDIIGNPHSSIFVPDQTLTIGGNFVKVVSWYDNEWGYSNRTAELMTKMGSL
                     "
     gene            3537..4730
                     /locus_tag="VHS97_09350"
     CDS             3537..4730
                     /locus_tag="VHS97_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX3448446.1"
                     /translation="MAKQTLRDINVTGRKVLVRVDFNVPQTADGAVADDRRIKSALPT
                     LTAIIDSGGSLILVSHLGRPTGDPAADSSFCMDKVAARLQELIGLPVLKVHDTVGSEA
                     QAACSALAPGGIVVLDNVRFNKGEKKGDAAFAKQMAALAECYVNDAFGTCHRDEASMV
                     AVPHQFAPDRRAIGFLVEKELGILETLLAYPRHPYVAVMGGAKVSDKISVIENLIKKV
                     DRLLIGGAMTYTFMRAQGKAVGNSKIEADKLDVARHVLDLAGEKLVLPQDHLVASKPE
                     AGAEIKVVSGDAIPDGWFGMDIGPATIAEYSAIIRQAGTVVWNGPMGKFEVEAFANGT
                     KAIAEALASSPAVTAVGGGESAEAIEKFGLADRVSHVSTGGGAFLEALEGKSFNSLAV
                     IPDRA"
     gene            complement(4767..5123)
                     /locus_tag="VHS97_09355"
     CDS             complement(4767..5123)
                     /locus_tag="VHS97_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448447.1"
                     /translation="MRGQRWFAIGVLAVLYGISSGCQGAGPRRTVVTPEPGLAGTIDG
                     DGTVTADGTRQVPPPKTVGWVERHPLFSKPRDYWETSGDNKIVKAAAATFVGVPVGFY
                     GEVKQIIVGAPTEPRY"
     gene            complement(5233..5784)
                     /locus_tag="VHS97_09360"
     CDS             complement(5233..5784)
                     /locus_tag="VHS97_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX3448448.1"
                     /translation="MSRYLWTRRCVGSVVLTVWLLAGSITARDSTDPTNVGLLRSQTT
                     RNQPAAEFDPTAACDEVVRAHNRLRAEAKLPPLAISTKLTAAAERHAKDMAAHEKMNH
                     KGSDSSSPIDRIKAEGYPYRRAGENIAAGRYNIERLMKVWVDSPHHKKNILGSFSQIG
                     VACATGESGKRYWCVTFGLPIRR"
     gene            6266..7045
                     /locus_tag="VHS97_09365"
     CDS             6266..7045
                     /locus_tag="VHS97_09365"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3448449.1"
                     /translation="MDVFTPTGTPNGLGIIDIASGSWYSDRNKIRDHTLAQVYTIFCS
                     RGYVVFAVRPGSKTRYTAAEMDGNVKSAIRFVKAHAGEFKIDPSRLGLTGGSAGGHLA
                     TLAALTPEPGKADATNPADRNETSVRAVGVFFPPTDFLDWDGNKAIRIDVLGPLLFVG
                     GVRGHSEDEIKAAAKAISPLHRVEKPTIPFLLIHGDADNVVPLSQSKKLVEALTKAGG
                     SAELIVKPGGGHPWLTLPEEVKVMADWFDRQLADKAIGGGQ"
     gene            7333..8193
                     /locus_tag="VHS97_09370"
     CDS             7333..8193
                     /locus_tag="VHS97_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX3448450.1"
                     /translation="MTEGCPSKREYVALDLETTGLMAATDRIVEIGAVRFRTDGQELC
                     RFQRLVNPERPMSPAAFAIHGLSDEVLAGAPPARDILPEFLVFLGDPGMTALLAHNSS
                     FDAGFLGCELMRAGLPIPTHSMIDTLALARRRLPWLASHRLDNVARALGLDPAGAHRA
                     LADCLRVKEIWLLLSGDSEPQDDVVSYRMFDRRDSEPTPEGWEDVLRAAARGTMIRIE
                     YDGGTRGSTPRSITPRRFVQRGGANYLIAFCHIDSLEKSFRLDRIRCVQLTFDPSSEP
                     VQVDRSAVRG"
     gene            8236..8814
                     /locus_tag="VHS97_09375"
     CDS             8236..8814
                     /locus_tag="VHS97_09375"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEX3448451.1"
                     /translation="MLTAFVLTVLAVLFRVFSATYQVWNFAPMAAISLYAGSRLPRRW
                     AWVVPVVAMVVSDLLLDQGRYRPAFELTRWTIYTTFAVTTLLGPLANWPRFGRWLLPI
                     LSVTGSLLFFVTSNLATWGEGLLYPMTFTGLIACFVAAIPFFGNTIAADLIGTAALFG
                     LGPVFERAARSVTGRRTAAVAQEITPSESRAA"
     gene            8834..9604
                     /locus_tag="VHS97_09380"
     CDS             8834..9604
                     /locus_tag="VHS97_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HEX3448452.1"
                     /translation="MAKLLVSVRSRDEALAALAGGADVIDVKEPLYGPLGRATPAVWR
                     EVREVVPPPIPLSVALGELNDWLDGDEENVAASSLVGVSYRKLGFSHAPPDWFARWQA
                     LCRRRSKHVSSPPAWVAVVYIDWQAARAPDPDSIIGATSELDECEGVLFDTWDKTGGM
                     RLDRTWKPRVDRVRDTGRFVALAGSLDIDAIKRLAVLEPDIFAVRGAACVGGDRLKTV
                     DSQQVSELARAVGHVTSASQHHSPTSNRTPCASGNSLP"
     gene            complement(9550..>9890)
                     /locus_tag="VHS97_09385"
     CDS             complement(9550..>9890)
                     /locus_tag="VHS97_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3448453.1"
                     /translation="PILYVMKYKTFNEAIAIQNAVEQGLTSAIFTNDLREAHRFLSPE
                     GSDCGIANVNIGTSGAEIGGAFGGEKATGGGREAGSDSWKAYMRRQTCTINYGSELPL
                     AQGVRFDVGE"
BASE COUNT         1907 a         2938 c         3027 g         2018 t
ORIGIN      
        1 ctcattgcgg ctttctgtcg gaaaaggcaa gataggcgtc ctaattgcaa tgtcgcttga
       61 agacgaccca tcttctttgg agagaaaggc aatcagagta cacgagcttc ggccaggatc
      121 ggtggatgtg tgccggcccg acccaaccgt cacggatcct acgacttccg ttttccgcga
      181 aaccagtgaa tcggttcagg gttaaattgg acggtgatgt caatcattcc cgaagggaaa
      241 cttgcaagcc tctgaccacg gatgagaccg aggctgatgt cgctgaccac catagagtgg
      301 ggccggcgca ggagccgccc aacacgattt cgccaacgca agtcagttcc gaacaaggct
      361 tatcgcctgg aaaccgcact ttaccgagct ccacgatgac tttgaggtat caaacgtgcg
      421 tgagatgggt tgaaagctac agcggattgg caaaatccgt gcggttcgat gaccttaatt
      481 cgttgacatt aagaggaccg tcaaatgggt attatggcct tccggatcga agaaacacac
      541 cgagacgttg gttaaaagag catgttgtct catcatggaa attgacgccg gcaaaacgcc
      601 aacccggaaa ggctcccgat ggtgtctcgg ccaagtctca atctccacga acgaacccaa
      661 gcggtgatgg ctcttcattc tatgatcgac agcttggtat acgtctcacg gaaatcggcg
      721 ggttggcgac taatgaggtt gcggcctatg acctccaggg ccatgcggag atcgaagcga
      781 acgtgctcgg atcgtcgctc ggtctcgcgt agacgcgagg gcgctggtcg atgaatgtcg
      841 ataggtttgc cttggtggcg ctcgttgtcg tgaacctggc gctcgccccc tacttcgtct
      901 tcctgctcgc gacctcgctt gcggctctcg cctcgcgtcg cgcgcggcga agctcggaag
      961 atccgcgctc gaggtttctg atcgtgattc ccgcgcatga cgaggaatcc ggcatcaaga
     1021 ccaccgtccg gagttgtttg gcggcggact acccgtcgtc actcttcggc gttttggtga
     1081 ttgctgacaa ttgctccgac cgcaccgcct acctggcggc ggaggcgggc gcccgggtcg
     1141 tcgagcgttt cgattccttg aaaaagagca aagggtacgc aatcgagtac ctgatggaat
     1201 cactggaact gtccggcgag ttcgacgcgt ttgacgccct cgtcatcatc gacgcggata
     1261 cgacgatgga tgccgatctg cttcgttact tcgaccaagg ccttcaagag ggtcgcgact
     1321 ggattcagtg ctactacacg gtggccaatc ccgatcagtc gtggcggact cgactgatga
     1381 cctatgcctt cagcttgttc aatggcgtca tgctcttggg gcaaaaccgg ctcggggcga
     1441 gtgccgggtt caaaggcaat ggaatgtgct tgaccacccg tggattgcga cgaagaccgt
     1501 ggaggtcgta cggactggtc gaggacatgg agtactcgtg gacgctacgg atcatgggtg
     1561 aaaaaatcgc gtttgcaccg gaggtcgcgg ttcacggcgc gatgctcggg tcaggcggcg
     1621 ccgccgcggc gaatcagcgg cgacggtggg agttcgggcg aggagatatc aggaagaaat
     1681 atctcgggcc gctccttcga tccaaccgga tcagctggtg ggaaaaagcc gtatcggcct
     1741 gcgaattaac cattccatcg atgggagtgt tagcactctt atatgttatt gtgctggtac
     1801 tcaacaccgt cgcctggttt gcaaacggca cttacctacc tcccgtcgcc cgtggattct
     1861 tgttggcttc ctgtatcttg atgacagcat cgatggtcgc ctatgggatt tccccattcg
     1921 tggtcatgcg gctcccctgg agatacgcct ggagcattgc gcttttcccg gtttacttga
     1981 tctggaagct gttgatttcg ttgaagggtc gaccccagca gtgggttcgc accgcccgcg
     2041 agtcacaaag agaagggccc cgtaacttca gggacagggt atcggcatcg aggaaactgg
     2101 gcgaatagca cccctgacag ttattcgtgg gcttgcgcgc tcccgccgac tcccgcgacg
     2161 tccttgccaa tggcggtata attcggtgtg atcttccaga gcgactggga gcgacgagga
     2221 cggcttggaa ttggcaggga tcgccgagtc tcgctctgct aaatcgtcgt cgggaagcca
     2281 aactctcgcc tggcaaaggg ccctgcagag taccggggcg ccttctacga aaacgggtac
     2341 ttttctgtta gtctaggccg gtcgatcgtg attcgaccgg tttcgactcg cgatatggat
     2401 ttcactgtgt tgaacgagga ggtcagtcgt ggcattgcgt gtggggatca atggatttgg
     2461 ccgaattggc cggctcgttt ttcgcgtcat ggctgagcgg cccaacgatt ttgacgtcgt
     2521 ggcgatcaac gatttgtcgg acccgaagca cctggccttg ctactgaaat atgacagcgt
     2581 tcaagggagg ttcaagggaa ccgtcgaagc tcgcgaccgc agcattgccg tcaacggcaa
     2641 agacatcccg atcactaccg agaaggatcc cgcccagttg ccctggaaaa aactgggctg
     2701 ccagatcgcg ctcgagtcga cggggcggtt taccgatcgg gccaacctgc aaaagcacat
     2761 cgacgctggt gcagagcggg tcgtcttgag tgcaccggca aaggatgacc tcgatgcaac
     2821 gatcgtcttg ggcgtgaacg acaagatctt gaacaagagt catcgcatca tctccaacgc
     2881 gtcgtgcacg accaattgcc tggccccctt ggccaaggtg ctgaacgagg ccttcgggat
     2941 cgagagcggc ctgatgacga ccgtgcacgc ctacacgaac gatcaacgtg tcgccgacca
     3001 gatccacgag gatccctatc gtgcccgcgc ggccgcggtg aatatcattc cggccaagac
     3061 cggcgccgct aaggccgtcg gtgaggtcat tcccgagctc aagggtaagc tgaccggctc
     3121 gtcacttcgc gtgcccgtgc ccgccggatc ggtcgtcgac ctcactgtgc tcttaaagac
     3181 gagcgcatcc cgcgaccaga tcaatggagc cttgaaggca gcggcggacg gcccactcaa
     3241 ggggatcctc gagtatgcca ccgacccgat cgtttcaagc gatatcattg gcaatccgca
     3301 ttcaagcatc tttgtccccg accagaccct gacgatcggc ggcaacttcg tcaaggtcgt
     3361 gtcctggtac gacaacgagt ggggctattc caaccggacc gccgagctca tgaccaagat
     3421 gggctcgctt taagataggc tggtgcagtc gcaatccgaa gtaggagcgc accggacgac
     3481 cccggccggc ggcgctcgtt cttcgtttcc ggatctctct gggaggaaca gcaaccatgg
     3541 caaaacagac tctccgcgac atcaacgtga ccggccgcaa ggtcctggtc cgcgtcgact
     3601 tcaacgtgcc tcagacagcc gacggtgcgg tcgccgacga ccgacgcatc aagagtgcct
     3661 tgcccacgtt gaccgcgatc atcgactccg gcggcagcct gatcctcgtc agccacctgg
     3721 gccgccccac cggagacccg gccgccgatt cctctttttg catggacaag gtggccgccc
     3781 gcctgcaaga actgatcggc ctgcccgtct tgaaggtcca cgatactgtt ggatccgagg
     3841 cgcaagccgc ctgttccgcg ctggcccccg gcggcatcgt ggtgctcgat aacgtccggt
     3901 tcaacaaagg ggaaaagaaa ggcgatgccg cgttcgccaa gcaaatggct gccctcgccg
     3961 aatgctatgt caacgacgcg ttcggcacct gtcaccgtga cgaagcgtcc atggtggcgg
     4021 tcccacatca gttcgcgcct gaccgccgcg cgatcggctt cttggtcgag aaggagctgg
     4081 gcatcctcga gactttgctc gcctatcccc ggcacccgta tgtggccgtc atggggggcg
     4141 ccaaggtctc cgacaagatc agcgtcatcg aaaaccttat caagaaagtc gaccggctct
     4201 tgatcggggg tgcgatgacc tacacgttca tgcgggctca gggcaaggcg gtcggcaata
     4261 gcaagatcga ggccgacaag ctcgacgtcg cgcgccacgt gcttgacctt gccggcgaaa
     4321 agctggtcct tccccaagac catcttgtcg cctccaaacc cgaggcaggt gccgagatca
     4381 aggtcgtatc cggcgacgcg atccccgatg gctggttcgg catggacatc gggccggcga
     4441 cgatcgccga gtattcggcg atcatccgtc aagccggcac cgtcgtctgg aacgggccga
     4501 tgggcaagtt tgaagtcgaa gcattcgcca acggaaccaa ggcgatcgcg gaggctctcg
     4561 ccagttcgcc ggccgtgacc gccgtagggg gaggggagtc ggccgaggcc atcgagaaat
     4621 ttggcctggc cgaccgagtc agtcacgttt cgactggcgg cggcgcgttt cttgaggcac
     4681 ttgaggggaa atcgttcaat tccctggcgg ttataccgga tagagcttga ttgccgtgac
     4741 acaaaggcca cttcaagtct aattcatcaa taccgtggtt ccgtcggagc gcccacgatg
     4801 atttgcttga cttcaccgta gaatccgaca ggaacaccca caaaggttgc cgcggccgct
     4861 ttgacgattt tattgtcgcc ggacgtttcc caatagtcgc gcggcttgga aaagagtggg
     4921 tgacgctcaa cccatccgac ggtttttggt ggcggcactt gacgggtgcc gtcggcagta
     4981 acggtaccat cgccgtcgat cgtgccggcc aggccgggct ccggcgtgac gacggtccgc
     5041 cgcgggccgg cgccctggca gccagagctt atgccataaa gaacagctaa cacaccgatt
     5101 gcgaaccagc gttgtccacg catcgtccct cgagcctcct attttgagat acgtgccatc
     5161 cgtggggacc gcgagcaatg cgcctgcgtc tcgatattga aacatacctg acaagccggc
     5221 ggagggagac gattaccggc gaatgggaag tccgaaggtg acacaccaat agcgtttccc
     5281 gctttcaccg gtcgcgcaag cgactccgat ctgggaaaaa ctgccgagga tgttcttctt
     5341 atgatgtggg ctgtccaccc agactttcat caggcgctcg atgttatagc ggccggcggc
     5401 gatgttttcg ccggcgcggc ggtaggggta tccctcggcc ttgatccgat cgatagggct
     5461 agagctgtcg cttcccttgt ggttcatctt ttcgtgggcg gccatgtcct tggcgtgccg
     5521 ctcggccgcc gcggtcaatt tggtgctgat cgccaggggc ggcaatttgg cttccgcccg
     5581 taaccggtta tgagccctga ccacctcgtc gcacgcggca gtggggtcga attccgccgc
     5641 aggctgattc cgcgttgtct gggaacgaag aagaccgacg ttcgtcgggt cagtcgagtc
     5701 gcgagcagtg attgaacccg cgaggagcca gactgtaagc accacggatc caacacagcg
     5761 ccgggtccac agataacgcg acatcttgga aattatcgta cctccaccag ctgagcgtcc
     5821 tttttccgga tgagtgtgcc gcgcgttcat gagcctggtt cacctggaca cagtcaatta
     5881 ttgtagcgtt gaacagagtg ggattctccg cgaaaatagg tgtggcacgt ggaaaaagtt
     5941 cccgtcaaga ctgcctcgtc tgctcttcat ttgatcgtcc gcgtcgagac cggctgaact
     6001 cgccttggcg gggacatacc agcgttgcaa tccccggcct atgctccctg ctgttacact
     6061 gggacggcgc gtgagtggtg cccggtccgt ggaaccgttc gctatcccaa catcccaaca
     6121 ggaacatttt atgagattcg ctcatgcctg cttcgcggcc gccgttacgc tgacggttgt
     6181 cgtcgcgtcc cacgcgggag acggcctccc gtacgaccag aagaaggacg tcgtctacgc
     6241 cgaggcccac ggcaccggct tgctaatgga tgtctttacg ccgacaggca cgccgaacgg
     6301 cttgggcatc atcgacatcg ccagcggatc gtggtattcc gaccgcaaca agatccgcga
     6361 ccacacgctg gcgcaggtct acacgatctt ttgttcgcgc ggctatgtgg tgttcgctgt
     6421 ccgcccgggc tcgaagacgc gctacacggc cgctgaaatg gacggtaacg tcaaatcggc
     6481 catccgcttc gtcaaggcgc acgccggcga attcaagatc gacccgtccc ggctcggcct
     6541 cacgggggga tcggccggcg ggcatctcgc gactttggcc gccctgacgc cggagccggg
     6601 aaaggccgac gccacgaacc cggcagaccg gaacgagact tccgtccggg ccgtgggcgt
     6661 gttcttcccg ccgaccgact tcctcgactg ggacggcaac aaggccatcc ggatcgatgt
     6721 ccttggcccg ctccttttcg tcgggggcgt gcggggacac tctgaggacg agatcaaggc
     6781 ggcggccaag gccatctcgc cgctgcaccg ggtcgagaag ccgacgatcc cgttcttgct
     6841 gatccacggc gacgccgaca acgtggtgcc gctgtcgcag tcgaagaagc tcgtcgaggc
     6901 gctcacgaag gccggcggct ccgccgaact gatcgtcaaa ccagggggcg gccacccatg
     6961 gctcacgctg cctgaagagg tcaaagtcat ggcggactgg ttcgacaggc aactggcaga
     7021 caaggcgatc ggcggcggtc agtaggcatg gggtgctact ggtggcaatc ggcccactga
     7081 cagatttttg ggggtttgaa accaattgcg atttccgcgc cggcggatgg gctcgtcgga
     7141 gtcggaaaac cgtttcgccc ggcacaagcg gtcggacgca tgcagttcag gggctgtgtt
     7201 ccgtcggaaa atggccacgc cacgactcgg accgttgcac atcgcccgct ggccgaggat
     7261 gtcattgcaa acattcgaga gagagacgat cctgaaacaa acgaccgaga tcggcaagtc
     7321 acggtgagtc gaatgacaga aggctgtcct tcaaaacggg aatacgtcgc gctcgacctg
     7381 gaaactaccg ggttgatggc ggcgacggac cgtatcgtcg agatcggggc ggtgcgattc
     7441 cgcaccgacg gtcaagaact ctgccgattt cagcgtttgg tgaatcccga gaggccgatg
     7501 tcgccggccg cgtttgcgat tcacggcctt tcggacgaag ttcttgccgg tgctccgcct
     7561 gcccgcgaca ttttgcccga gttcctggtg tttttgggcg atcccggcat gacggctttg
     7621 ctggcgcata attcctcgtt cgatgccggt ttcctcgggt gcgaattaat gcgggctggt
     7681 ctgccgattc cgacgcattc catgatcgac acattggctc tggcgcggag gcgactgccg
     7741 tggctggcaa gccatcggct cgacaatgtg gctcgagcgc taggactcga cccggcgggc
     7801 gcgcaccgcg cattggccga ctgcttgcgg gtcaaggaga tctggctact tctcagtgga
     7861 gattccgagc cgcaagatga cgtggtctcg taccgaatgt ttgaccgcag agactcagag
     7921 ccgacacccg aaggatggga agacgtcctc cgggcggcgg ctcgtgggac tatgattcgg
     7981 atcgaatacg acgggggaac gcgcggttcg actccgcgat cgatcacccc gaggcgcttc
     8041 gtccagcgag gcggcgcgaa ctacctgatc gcattttgcc atatcgactc actggaaaaa
     8101 tcgtttcggc tagaccgaat tcggtgcgtg caattgacct tcgatccgag ctcggaaccg
     8161 gttcaggttg atcgctcggc tgtgcgcggt taaattacat ttctcgaaac aaatcaaatt
     8221 aacgaggacc ggttcatgct taccgcgttt gtgttgaccg tgctggccgt ccttttccga
     8281 gtgttttccg cgacctatca ggtctggaac ttcgcgccga tggcagcgat ctcgctttat
     8341 gccggatctc gtctgccacg ccgatgggcg tgggtcgtcc ccgtcgtggc gatggtcgtc
     8401 tccgacctgc tcctggacca agggcgttat cggcccgcct ttgagcttac ccgctggacg
     8461 atctacacta cttttgccgt cacgactttg ctcggaccgc tggccaattg gccgaggttc
     8521 ggccgctggc tgctgccgat tttgtcagtg accggttctc tcctcttttt cgtgacgagc
     8581 aatctggcga cgtggggcga aggtttgttg tatccgatga ccttcaccgg cctcatcgca
     8641 tgctttgtcg cggcaattcc gttcttcggc aatacgatcg cggcggacct gattggtaca
     8701 gccgcgctct ttgggctcgg tccggttttc gagcgtgcgg cccgaagcgt aaccggtcgc
     8761 cgcacggccg ccgtcgccca agaaataaca ccttccgaat cccgggcggc ctgacccgat
     8821 tcgggccatt cacgtggcaa agctgctagt cagcgttcga tcacgggacg aagccttggc
     8881 cgcgctcgcg gggggcgcgg acgtgatcga cgtcaaggag ccgctttacg gcccactggg
     8941 ccgagcgacg cccgccgtat ggcgcgaagt gcgtgaagtc gtgccgccgc caatccccct
     9001 gagcgtcgcg ctgggggaac tcaacgattg gctcgatggg gacgaagaga acgtcgcggc
     9061 cagctcactc gtcggggtta gttaccgcaa gctcggcttc tctcacgcgc cgccggattg
     9121 gttcgcccga tggcaggcac tttgtaggcg gcggagcaag catgtctcct cgcctccggc
     9181 ttgggtcgcg gtggtttaca tcgactggca agccgcgcga gcgcctgacc cagattcgat
     9241 tatcggggcc accagcgagc tcgacgaatg cgagggagtg cttttcgaca cctgggacaa
     9301 gacaggcggc atgcgcctcg atcgcacctg gaagccgcga gtggatcggg tgcgcgatac
     9361 agggcgattc gtggctctcg ccggctcgct cgacatcgac gcgatcaagc ggctcgcggt
     9421 gttggagcct gacatcttcg cggtgagagg agcggcgtgc gtcggcggtg accgactcaa
     9481 aaccgttgat tcgcagcagg tgtctgaact cgcgcgggcc gtcggacatg ttacgtcagc
     9541 ctcacagcat cattctccca catcgaaccg gaccccctgc gccagcggca actcgcttcc
     9601 gtagttgatc gtgcaggttt ggcggcgcat ataagctttc cacgagtccg agccggcctc
     9661 gcgcccgccc cctgtggcct tctcgcctcc gaacgcgccg ccaatctccg ccccggacgt
     9721 gccgatgttg acattggcga tcccgcaatc agaaccttcg ggcgaaagga aacgatgcgc
     9781 ctcgcgcaag tcgtttgtga agatcgccga cgtcagtccc tgctcgaccg cgttttggat
     9841 cgcgatcgcc tcgttgaacg ttttgtactt catcacatag aggatcggcg
//
LOCUS       DATAQL010000543         4313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2649781, whole genome shotgun sequence.
ACCESSION   DATAQL010000543 DATAQL010000000
VERSION     DATAQL010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649781"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..662
                     /locus_tag="VHS97_09390"
     CDS             102..662
                     /locus_tag="VHS97_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3448454.1"
                     /translation="MALVKRSVMGPNADGKSAILYRDTPNEQDVPNVVRRTTLWATTE
                     LPIDNAIRGDRGADVTVREPTEDGMIFRVLEIPPDMKDRKKHIELMKVVHRKVKQKYP
                     PTDKDLERHPTMHRTDTLDCFVVMRGEVYLVSDTDEVLLRAGDTAVVQGVNHAWSNRS
                     NEPVLIAGVMTHAKALPKEQYPAPGL"
     gene            801..2414
                     /locus_tag="VHS97_09395"
     CDS             801..2414
                     /locus_tag="VHS97_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3448455.1"
                     /translation="MHAIGISEVFGVPGDYAFPVQDAIVNFPGIEWVGGCNELNAAYA
                     ADGYARIRGLGAVSTTYGVGELSAINAIAGAYAEHLPVFHLVGMPNMATQAGRRLVHH
                     TLGNGEFDLFRKMAEPVVCASAILTPQNVAAETERLIAEALYHRRPVYLAIPADVANQ
                     AVLSTAPPRDPAMSDRSALRSATDAILTALKQAHTACILPGLLASRAGIRAELQSFVD
                     ASGLPFATMFMDKSVLEEQQPAYIGMYDGRLMDESVRRFVESCDRVLAIGAMMTDLNT
                     GAFTSRLDPEKTIEIRHHWTQVGPKVYQNVEMKDILAALTGRVTARRGKSPTQPGSLG
                     PAMGSGSDPITVEALYPRWANFLKPNDILVAETGTSSMGLAFALMPKGATFHNQTLWG
                     SIGWATPAAFGAAVAARDRRVVLVTGEGSHQLTAQETSQFGRRGLKPIVFVLNNSGYL
                     IERLLCKNPAIAYNDVASWRYSEVPHALGCDGWFTARVTTCGELDQALRAVEQGGSAA
                     YIEVVTDAYAASPLAKRMHESVETLYSHS"
     gene            complement(2617..3633)
                     /locus_tag="VHS97_09400"
     CDS             complement(2617..3633)
                     /locus_tag="VHS97_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448456.1"
                     /translation="MTAVFCANTADRKAQTWEQQLRRFDCLEFVVSDAAKGIAKAVAL
                     VAQSRRDDPRAKTLEHGLDIFHTAMEANRVLAQHWRRAEAAWEKAEAADTAVAASKRQ
                     GIDARGVAQTARAAWLQAIASLEQTERREAAWDRAHAALDLFGTDGRLNDCGYAKVEI
                     AEALKGLTGPEWSKVRNFLTDPRSLTFLDRMHRRLEEAEPDPQWREAMAWRWWLRHGR
                     VPSCDPLLRLVRAVGRDRVLNESEQTSYERVSAVLGSTFRASSAVECMNSVLRMQQSR
                     HRRMTQPMLDLKRLYWNCHRFGSGPRKEACPYQSLGLKLPTFDFWTLLQAKPEELIQR
                     LSTA"
     gene            complement(3630..4109)
                     /locus_tag="VHS97_09405"
     CDS             complement(3630..4109)
                     /locus_tag="VHS97_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448457.1"
                     /translation="MNTLSLRTIVRNAAAVFSGSYGAITRQAEQAGCSRQTVYDHAHQ
                     LEQRLQLAAPPRPPAEIGPPTPAPSLDESTQQRLAVTAFAMGISTRQIEDLLRVILSD
                     EGPDHATIGRWVAEAAKAAKPVLEILDAACIPQVQTLVVDEIFFGGDRPWSESNRRA"
BASE COUNT          786 a         1364 c         1384 g          779 t
ORIGIN      
        1 cgagggcgtg cggggcgttt ccgaatgggc ccgcccgccg gtccccgaga gcggctttcc
       61 caactgaaat cgcccgacac cctcaacaaa ggagacttgt catggctctg gtcaagcgta
      121 gcgtcatggg tcccaatgcc gacgggaagt cggcgatcct ttaccgcgac acgccgaacg
      181 agcaggacgt cccgaacgtc gtccgtcgca ccaccttgtg ggcaactacg gaactgccga
      241 tcgacaatgc gatcaggggc gaccgtggcg ccgatgtcac ggtccgggag ccgacggaag
      301 acgggatgat tttccgcgtg ctcgagattc cgcccgacat gaaggacagg aaaaagcaca
      361 tcgagctgat gaaagtggtg caccggaagg tgaagcagaa ataccccccg accgacaaag
      421 acctggaacg gcacccgacg atgcaccgca ccgacacgct ggactgcttc gtggtgatgc
      481 ggggcgaggt ttacctggtc tcggacacgg acgaggtcct gctgcgggcg ggagacacgg
      541 ccgtggtgca aggcgtgaat catgcctgga gcaatcgctc caacgaacca gtcctgatcg
      601 ctggcgtcat gacacacgcg aaggccctgc ccaaggagca gtatccggcg ccaggcctgt
      661 aaacagccca accgatggcg agccctcggc caggcgcggt ttgagggccc gccatcccag
      721 ccgcgatacc aggaaggagc gaaatcatga cggcgagtac cgcgaagcac cggacggtca
      781 ttgaataagt tctctcacgt ctgcatgcca tcggcatctc cgaagtcttc ggagtacccg
      841 gagactacgc ctttcctgtg caagatgcca tcgtgaactt cccgggaatc gagtgggtcg
      901 ggggctgcaa tgagctgaat gccgcgtacg ctgccgacgg ctatgcacgc attcgcggcc
      961 ttggcgcggt cagcacaacc tacggggtag gggagctgag cgcgatcaac gccatcgccg
     1021 gagcctatgc ggagcacctg ccagtctttc acctggtagg gatgccgaac atggcgacgc
     1081 aagcgggccg caggctcgtc catcataccc tcggcaatgg tgagtttgat ctctttcgga
     1141 agatggcgga acctgtggtg tgcgccagtg ccatcctgac gccacagaat gtcgccgccg
     1201 agaccgagcg gttgatcgcg gaggccctct atcaccgccg gccggtctac ctggccattc
     1261 cggcagatgt cgcgaaccag gcggtgctga gcacggcccc gccacgggat ccagcgatga
     1321 gtgatcgctc ggctctccgg tccgcgacgg atgcgattct cacagctttg aagcaagcgc
     1381 acaccgcgtg catcctgccg ggcctactgg cctcccgagc aggcatacgc gcggagctac
     1441 agtccttcgt cgatgcgtcc ggtctaccct tcgccaccat gttcatggat aagtcagtcc
     1501 ttgaggaaca gcagccggcc tacatcggca tgtatgacgg ccggctcatg gacgagtccg
     1561 tccggcgctt cgtggagtcg tgtgaccggg tcctcgccat cggagcgatg atgacggacc
     1621 tcaacacggg agccttcacc tcgcgcctgg atccggaaaa aaccatcgag atccgtcatc
     1681 attggacgca ggtcggaccg aaggtctatc aaaacgtcga aatgaaggac atccttgctg
     1741 cactcacggg gcgggtcacc gcgcgccgtg gcaaatcgcc gactcagcct ggttcgctcg
     1801 ggccagcaat gggcagtggc agtgacccga tcacggtgga ggcgctctat ccgcgctggg
     1861 caaatttcct gaagccgaac gacatcctcg ttgccgagac ggggacatct tcaatgggcc
     1921 tggcctttgc cctcatgccc aagggggcga ccttccataa tcagaccctg tggggctcga
     1981 tcggatgggc aacgccggcc gcctttggcg ctgccgtggc cgcccgggat cgtcgtgtgg
     2041 tgttggtgac cggcgagggg tctcaccagc ttaccgctca ggagaccagc cagttcgggc
     2101 gacgcggtct gaaaccgatt gtcttcgtgc tcaacaactc gggctatctg atcgagcgcc
     2161 ttctctgcaa aaaccccgcc attgcctaca acgacgtggc ctcctggcgc tattcggagg
     2221 tcccgcacgc gctgggctgc gacggctggt tcacggccag ggtcaccacc tgcggtgagc
     2281 tcgatcaagc cctgcgagcc gtcgagcaag ggggctccgc ggcgtacatc gaagtggtaa
     2341 cggacgccta cgccgcttcg cctctggcga agaggatgca tgaaagcgtc gagacgcttt
     2401 actcccactc ctaacgctgc tcgacgcgcc taataaggtg tcccccgtgt cctctggggg
     2461 gtagaacgct gcgcgagtcg gtcggatcgc ccgaggttgt cgagaatggc ttcggtacgg
     2521 cggaccgaag cgcgcaagac tcctgcaacc tctcggggct gcgcctcttt ggccttcgcg
     2581 atgagcagtt tcttggggac tgtctcatac cggaattcaa gcggttgaca gtctttgtat
     2641 caattcctct ggcttggcct ggagcagtgt ccagaagtcg aacgtgggca acttcaggcc
     2701 cagggattgg tacgggcagg cctcctttcg cggccccgag ccgaatcggt gacagttcca
     2761 gtagagccgc ttcaggtcga gcatcggctg cgtcatccgg cggtgccgcg actgctgcat
     2821 ccgcagcaca ctgttcatgc actccacggc gctgctggcg cggaacgtgc tccccagcac
     2881 cgccgagact cgctcatagg acgtttgttc cgactcgttc aacacacgat cacgcccgac
     2941 tgctcggacc agtctcaaga gcgggtcgca ggacggcact cggccgtgcc tcagccacca
     3001 acgccaggcc atcgcctcac gccactgcgg atcaggttca gcctcttcca ggcgccggtg
     3061 catccggtcc aggaaggtca ggcttcgcgg gtccgtcagg aagttgcgga ctttcgacca
     3121 ctccgggccc gtcaggccct tgagcgcctc ggcgatctcg accttggcat agccgcagtc
     3181 gttgaggcga ccgtccgtac caaacaggtc cagcgccgca tgggctcggt cccaggccgc
     3241 ttctcgacgc tcggtttgtt ccaacgacgc gatcgcctgg agccaggcgg cgcgggcggt
     3301 ctgggccacg ccccgcgcgt cgatcccttg ccgtttcgac gcggcgaccg cggtgtcggc
     3361 ggcttccgcc ttctcccagg cagcctcggc tcgccgccag tgctgggcca ggacgcggtt
     3421 ggcctccatg gcggtgtgga agatgtccag gccgtgctcc agggtcttgg cccgaggatc
     3481 gtcgcggcgg gattgggcca cgagtgcgac cgccttggcg atccccttgg cggcgtcgga
     3541 gacgacgaac tccaggcaat caaaccgcct gagttgttgc tcccaggtct gggctttgcg
     3601 gtcggcggtg ttggcgcaga agacggcggt catgctcgcc ggttcgattc cgaccaaggt
     3661 cggtcgcccc caaaaaagat ctcgtcgact acgagcgtct gaacctgggg gatacacgcc
     3721 gcgtcgagga tctcaagaac cggtttggcc gccttggccg cctcggcgac ccaacgtccg
     3781 atggtggcgt gatcgggacc ctcgtcgctg aggatcacac gcaggaggtc ttcgatttgg
     3841 cgagtgctga tccccatggc gaatgcggtc accgccagcc gttgctgagt ggactcgtcg
     3901 agggaggggg ccggcgtggg agggccaatc tcggccggag gccgtggggg agcggcgagt
     3961 tgcaaccgct gttcgagttg gtgggcgtga tcgtagacgg tctgtcggct acagccggcc
     4021 tgctcggctt ggcgagtgat cgcaccgtac gatccggaga agaccgcggc ggcgttgcgg
     4081 acgatggtgc gaagcgatag cgtgttcatc ggggaggccc tctgtgccgc tggcgatcgt
     4141 ggttgtgaac cagtcgatca tggggtggcc tccccttcaa gacaaactgt caactcgacc
     4201 aggctgttgg tagcccaatt tgcgacagag ccaaacaatc gagaatcgac cctaacctaa
     4261 cgcgattcgc ccctctgcct tacgtcaggt tggagtgcac ccccaactag acg
//
LOCUS       DATAQL010000544         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5092351, whole genome shotgun sequence.
ACCESSION   DATAQL010000544 DATAQL010000000
VERSION     DATAQL010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5092351"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VHS97_09410"
     CDS             complement(<1..617)
                     /locus_tag="VHS97_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448458.1"
                     /translation="MIVAAVVILALAQAPAGSTTLSGQVLDAGGRPAVGVDVLLSGLG
                     QRTGRPVLSRAKSDQEGRFRIDVPAEKDLGRARFLLALWAYSPKEGLAGQAFSPSALP
                     APGSLQLKLGGPAHTAVRVSGPDGKPVAGARVAPVMVRVVGGVRPRSTFPPPDSLADL
                     LAATTDADGKGQMHGCRAEDVEAVWVEAAGFGRQGSELSPGAGGD"
     gene            complement(807..>2302)
                     /locus_tag="VHS97_09415"
     CDS             complement(807..>2302)
                     /locus_tag="VHS97_09415"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEX3448459.1"
                     /translation="LNTPEQRRLGVKDSYELALGDWMGTAGFDRAEDYWPRQWAEAFV
                     AFAAGEARSWLHQQGMRWFPIVGWAERGGYGAFGHGNSVPRFHITWGTGPGVLEPFVR
                     RTTAAEKMGLIAFKFRHRVDELLRTNGAIDGVRGAILEPSDVPRGVKSSRTVVGEFTL
                     RAGAVIVASGGIGGNHDLVRQNWPKRLGNPPKFMVQGVPDHVDGRMLFITETAGARLI
                     NRDRMWHYTEGLRNWNPIWTNHGIRVLPGPTSLWLDATGKRLPPPLYPGFDTLGTLEY
                     IAKSGHEHTWFLLNQAIIKREFALSGSEQNPDLTGKDWRQTLGRAFSKTATGPVEAFK
                     KHGADFVVKDNLADLVAGMNALSAAPLLEFATVEREVTARDRQLHNPFGKDAQLAVIR
                     QARKYIGDRLIRVAKPHALLDPKAGPLIAVKLNILTRKTLGGLETDLCARVFGTDGAI
                     LPGLYAVGEAAGFGGGGVHGYRSLEGTFLGGCIFSGRNAGRAAAATV"
BASE COUNT          423 a          795 c          707 g          377 t
ORIGIN      
        1 tggtcgccgc cggcgcccgg gctgagttcg cttccctggc gtccgaaccc ggcggcctcg
       61 acccagaccg cctcgacgtc ctcggctcga cagccgtgca tttggccctt gccgtcggcg
      121 tcggtcgtgg ccgcgagtaa gtcagcgagt gagtcgggcg gcgggaacgt cgacctgggc
      181 cgaacccctc cgaccacgcg caccatcacc ggcgccactc gggcgccggc caccggcttt
      241 ccatcaggtc cggacactcg aaccgcagtg tgcgctgggc cgcccagctt cagttgcaac
      301 gaacccggtg ccgggagcgc tgacggcgag aacgcctgac cggcgagccc ctccttcggg
      361 gagtaggccc agagcgccag gagaaaacga gcccgcccta gatccttttc ggccggaacg
      421 tcgatccgga accgcccctc ttgatcggac ttcgcccgcg aaagcaccgg ccgtcccgtc
      481 ctttgtccta gtcccgaaag caagacatcg acgccgaccg cgggccggcc gccggcgtcc
      541 aacacctggc ccgagagagt tgtcgagccg gctggagcct gcgccagagc caagatcaca
      601 acggcagcga cgatcatgga gacctccaca atcccaagcg ccacactcgc gcgaggcggg
      661 cattcgagcc agcatccgcc tttccgccga aaaccaaaac cactttacga tcgccgatca
      721 taccacaatt gtcgttggtc agggtaaaaa aacggacacg agaattaaga agggcaaatt
      781 ccatgatccg cccgcgattt gacgtttcaa acagtcgccg ccgcagctcg ccccgcgttg
      841 cggccggaaa agatgcagcc gcccaagaac gtgccctcaa gcgaccggta gccgtgcacg
      901 ccgcctccgc cgaagccggc cgcctcgccc accgcgtaga gaccgggcag gatcgcgcca
      961 tccgttccga acacgcgggc acagaggtcg gtttccaagc cccccagcgt cttgcgggtg
     1021 aggatattga gcttgaccgc gatcagcggc ccggccttgg ggtcaagtag cgcgtggggc
     1081 ttggccacgc ggatcagcct gtcaccgata tatttgcgcg cctggcgaat gacggcgagc
     1141 tgcgcgtctt tgccgaaggg attatgaagc tgccggtcgc gggcagtgac ctcgcgctcg
     1201 accgtggcga attccaagag cggagcggcc gagagcgcgt tcattccagc caccagatcc
     1261 gccaggttgt ctttgacgac gaagtcagct ccatgcttct tgaacgcctc gaccggtccc
     1321 gtggcggtct tgctgaaggc tcgcccgagc gtctggcgcc agtctttgcc ggtcaagtcc
     1381 gggttttgct ctgagccaga gagagcaaac tcgcgcttga tgatcgcctg attgagcaag
     1441 aaccaagtat gctcgtgacc cgacttcgcg atgtattcga gagtgcccag tgtgtcgaaa
     1501 ccgggataca gcggcggcgg caggcgctta cctgtcgcgt ccagccataa tgacgttgga
     1561 ccgggcagga cgcgaatgcc gtggttggtc cagatcgggt tccagttcct gaggccctcg
     1621 gtgtagtgcc acatccgatc gcggttgatc agccgggctc cggccgtctc ggtgataaac
     1681 agcatccgcc cgtccacatg gtccggcact ccctgcacca tgaacttggg cggattgccc
     1741 aggcgcttcg gccaattctg acgtaccaga tcgtggttgc cgccgatgcc accggaggca
     1801 acgatgaccg cgccggcgcg caaggtgaat tcaccgacga cggtccgcga actcttcaca
     1861 ccccggggca cgtcgctggg ctcgaggatc gccccgcgca cgccgtcgat cgcgccattc
     1921 gtgcgcaaga gctcgtcgac gcggtgccgg aacttgaagg cgatcaggcc cattttctcg
     1981 gccgcggtcg tccggcgcac gaaaggctcg agcacccccg gccccgtgcc ccaggtgatg
     2041 tgaaatcgcg gtacggaatt gccgtggcca aaagccccgt atccgcctcg ctcggcccat
     2101 cccacgatcg gaaaccagcg catcccttgc tggtgcagcc acgaccgcgc ctcaccggcg
     2161 gcaaaggcga caaaggcctc cgcccactgc cgcggccagt agtcttccgc gcggtcgaag
     2221 ccggctgtcc ccatccagtc gcccaaggcg agctcgtaag agtctttcac gcccagccgg
     2281 cgctgttcag gagtattcag aa
//
LOCUS       DATAQL010000545         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1579973, whole genome shotgun sequence.
ACCESSION   DATAQL010000545 DATAQL010000000
VERSION     DATAQL010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579973"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VHS97_09420"
     CDS             <1..212
                     /locus_tag="VHS97_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX3448460.1"
                     /translation="HTLCIELAPEVFDLGDDAANFEETPQESSRQRVESAVAAYPRQA
                     ISVVFGETRADTPLGAQLTSDLERT"
     gene            216..1493
                     /locus_tag="VHS97_09425"
     CDS             216..1493
                     /locus_tag="VHS97_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX3448461.1"
                     /translation="MTDLADVDYFTNSEVAQDPYEYWDYLRSQGPVFREPHHGVVAVT
                     GYQEVQTAFKDVDSFSAVNAIGGPFPPLPFIPAGDDISEQIDAHRHEFPIFEHMVVMD
                     PPQHDKARSLLGRLLTPRRLQENTDYIWQLADRQFDEFIAEGRCEFLGDYGKPFATLA
                     IADLLGVPQADRAEIRRNLGAGRAPGGRVGALDHEPVGSNPLQYLDDLFSGYISERRQ
                     NPRDDVLTGLATANYPDGSTPPLLEVVRPATFLFAAGQETVTKLLSSAVQVLGDQPEL
                     QDRLRTERNLIGPFIEEALRMQSPTKVDFRLARKTTTLGGVHIPAGTVLMLCLGAANR
                     DPRKFENPNQFQVDRKNVREHIAFGRGIHTCAGAPLARVEGKVTINRLLDRTHDISIS
                     AAKHGPTDLREYRYAPTFLLRGLTELYIEFTPA"
BASE COUNT          339 a          544 c          463 g          306 t
ORIGIN      
        1 cgcacaccct ttgtatcgag ctcgcgcctg aggtcttcga tctcggtgat gatgccgcga
       61 actttgaaga gacaccccaa gaatcgtcgc gacaaagggt ggaatccgcc gttgccgcgt
      121 atccacgtca agcaatcagc gtggtctttg gagaaacccg agccgacacg cccctgggcg
      181 cccagctcac ttccgatctg gaaaggacct aacggatgac cgacctcgcc gacgtcgact
      241 acttcaccaa cagtgaagtc gctcaagacc cctacgaata ctgggactac ttacgcagcc
      301 aagggccagt gttccgcgag ccgcaccatg gcgtcgtggc cgtcaccggc tatcaggaag
      361 tccagaccgc atttaaagac gtcgattcat tctccgccgt caacgcgatc ggcggcccat
      421 tcccgccgct gccattcatc cctgccggcg acgacatcag cgaacagatc gacgcgcatc
      481 gtcatgaatt tccgatcttc gaacacatgg tcgtcatgga cccgccccag catgacaagg
      541 cgcgttccct cttggggcgg ctcttgactc cccgtcggct gcaggaaaac acggactata
      601 tctggcaatt ggcggaccga cagttcgacg agttcatcgc cgagggccgc tgcgaattcc
      661 tcggcgatta cggtaaaccc tttgcgacct tggccatcgc cgatctcctt ggagttccac
      721 aggcggaccg cgctgagatc cgtcgcaatc tcggagctgg cagagcgccg ggtgggcggg
      781 tgggcgcatt ggatcatgag cccgtgggca gcaatccgct gcaatacctc gatgacctgt
      841 tcagcggtta tatctccgag cgacgccaaa acccccgcga cgacgtgctt acaggtctag
      901 cgaccgccaa ctatcctgac ggctcgaccc cgccgctgtt ggaggtggtc agacccgcga
      961 cattcctgtt cgcggcaggc caagagaccg tgaccaagct gctcagctcg gcagtgcagg
     1021 tcctcggcga ccagcccgag ctccaggacc gactgcgtac cgagcgaaac ctgatcggtc
     1081 cctttatcga agaggcctta cgcatgcaga gcccaaccaa ggttgacttc aggctggcgc
     1141 gcaagaccac cactcttggc ggagtacata tcccggccgg cacagtgctc atgctgtgcc
     1201 tgggtgccgc gaaccgtgat ccgcgaaagt ttgaaaatcc caaccagttt caggtagacc
     1261 gtaaaaacgt tcgcgagcac atcgccttcg gccgaggtat ccacacctgc gcgggtgcgc
     1321 cgctcgcccg agtggaaggg aaagtcacga tcaaccgtct gctcgaccgg acacacgaca
     1381 tttcgatcag tgcggccaag cacggtccga cggatctccg cgagtaccgt tacgcgccga
     1441 cgtttctcct gcgtggcttg actgaactgt acatcgagtt cacgccagct tgagctacaa
     1501 ggcgggcact gaccgccgaa ggggtcaccc cccaccctct ccgagcactc ctaattggtc
     1561 gagcagcgtg cgtacccgct gttcgatctc gtcgcggatt ggccgtatcg cctcgagtga
     1621 ttgaccggcc gggtcggcaa gaacccagtc ct
//
LOCUS       DATAQL010000546         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5092494, whole genome shotgun sequence.
ACCESSION   DATAQL010000546 DATAQL010000000
VERSION     DATAQL010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5092494"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..361
                     /locus_tag="VHS97_09430"
     CDS             137..361
                     /locus_tag="VHS97_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448462.1"
                     /translation="MTSSTARIAVKLHKNVTLVRTTEPVLAEELLARKTLARWVVGRL
                     SDTVLLVLPDEADAVIEELRRMGQTPRVVR"
     gene            451..2694
                     /locus_tag="VHS97_09435"
     CDS             451..2694
                     /locus_tag="VHS97_09435"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX3448463.1"
                     /translation="MTSVEPRLAFRGALIRYKGSRLAAIRLAQGAAPEGARIRTFGNV
                     DEIVDYLDSPSAVEALISRLDIGSRLALCVFAVSETASHSFAGLSHALGVLGADPTPA
                     IVKLLELGLLVIEPNPELSPVDDFQSVFLRVSPGRLRLRVHPSVANGIRTARPDLAPP
                     KVAGPVGQVRESDGLEPILRLGALWQRVGAEPLRQTNQGTLYKRDRDRLADDPVLLDP
                     IADAFKPIARAPELWLELACRVGLVEPDSTGERILAASPEFWTDNAVHLPQMIATKWL
                     GLETWQELESTPSGGIATVPALPYLRFALLLWLSALEFTEWAALDDLAAQLSERSSTW
                     DRLSLAEEGDASLAVQRREGPAAGRARNRSRPDGVPRGVSVLESVLLGAAYPLGLIRA
                     GNEQGSGRIVVQITPLARYVLALGPTPPPRTTFEQFLFVQPNFEVIAYRQGLTPQLVG
                     RLSQFAWWSQIGAALELRLTRESIVLGLDGGLTPEAILQTLTRHSQRALPAGVTDAVT
                     NWASRRERVTYYAAATLIEFGSSSERDLALANWPVTVLAAPIAVAERFLLVEDEKTVP
                     FDRLRLISSRDYRRPPEICVLVQPDGVTMTLDPSRSDLLVEAELARFAEVLSAPQPEP
                     SQMAPTVLRRFAVTAASLRRATNRGMTSPQLIEWYSRRTGGEIPPAVRLLLAARTSRM
                     PILKAARMLILDLPTAELLDGLLQHPATSPWLGDRLGPTAVAIADDRLAPLQKALKDL
                     GIELQAL"
     gene            complement(2897..>3134)
                     /locus_tag="VHS97_09440"
     CDS             complement(2897..>3134)
                     /locus_tag="VHS97_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448464.1"
                     /translation="VPGAPASVKTKAEPTDLNLDPSADHDHFGPPSGDAQTQEGTGDQ
                     ADSKPATPQGATSGSRWRRVKAKPALEAAPSAER"
BASE COUNT          522 a         1026 c         1008 g          578 t
ORIGIN      
        1 cctacgagat cctcgacgaa accgacctgg ccgagcgtgg tcggatgtgg ccagctgcca
       61 ctgggtgagc gggaccgttc gaatcgactg gctatttcga ggcagaatcc cgatcacgac
      121 ttttggtagg acgacgatga cctcctcgac tgcccgaatc gctgtcaagc tgcacaaaaa
      181 cgtgaccttg gtccgcacga ccgagccggt gctggcggag gaactgctgg cccgcaagac
      241 gctggcgcga tgggttgtcg ggcgattgtc agacacggtg ttactggtcc tgccggatga
      301 ggcggacgcc gtgatcgaag agcttcgccg catgggccag acgccgcgcg tcgtgcgcta
      361 gagggacttt ttgagatggc tgctctgtcc caatcgtccc cgagccgccg ctgggccgac
      421 ggccgaatga ttgtcgggag gtccggggat gtgacgagcg tggaaccccg gctggcgttc
      481 aggggggccc tgattcgcta caaaggatca cgtctggccg ctatcaggct ggcccaggga
      541 gccgcgcccg aaggcgcgcg aattcgtacg ttcggcaacg tcgacgaaat cgtcgactac
      601 ctcgacagcc catccgcggt cgaggccctg ataagccgac tggacatcgg atcgcggctg
      661 gcgctctgcg tctttgcggt ctctgagacc gcgtcgcatt cattcgccgg cctgagccac
      721 gccttgggag tgctaggagc cgaccctacc cccgcgattg tgaagctcct cgagctgggc
      781 ttactggtga tcgagcccaa tcccgagctc agccccgtgg atgatttcca gtcggtgttc
      841 ttgcgtgtga gtccgggccg gctccggcta agggttcacc caagtgtcgc aaacgggatc
      901 cgtacggcgc ggcccgacct ggcgcccccc aaggtcgctg gcccggtcgg ccaggtccgc
      961 gagtcggacg ggctcgaacc gatcctccgg ctgggtgcac tctggcaacg agtaggagcc
     1021 gagccgcttc gtcagaccaa ccagggcacg ctctacaagc gcgatcgcga caggctcgcg
     1081 gacgacccag tgctgctcga cccgatcgcc gacgcgttca agccgatcgc tcgcgcgccc
     1141 gagctgtggc tcgagcttgc gtgccgcgtc ggcctggtcg aaccggactc gacgggcgag
     1201 cgtatcctcg cggcatcgcc agagttctgg accgataacg cggtgcactt gcctcagatg
     1261 atcgccacca aatggttggg gctggaaacg tggcaagagc tggaatcgac gccatccggc
     1321 ggcattgcga cggtgccggc gttgccatat ctccgcttcg ctctgctgct ctggttatcg
     1381 gcactcgaat ttaccgagtg ggccgccctg gacgatctgg ctgcgcagct ttcagagcgc
     1441 tcgtcaacat gggaccgact gtctttggcc gaggaaggcg acgcgtcgct cgccgtgcag
     1501 cgacgagaag gccccgctgc cggccgggct cgcaaccgga gtcgccccga cggcgtgccg
     1561 cgcggcgtca gcgtgctcga gtcggtcttg ctcggcgccg cttacccgct cggcttgatc
     1621 cgcgccggca atgaacaggg gagcggtcgc attgtcgtgc agatcacacc gctggcacgc
     1681 tatgtcctgg cactcgggcc gaccccgccg ccgcgcacga cgttcgaaca gttcctcttc
     1741 gtgcagccca atttcgaggt cattgcctat cgccaaggat tgacacctca gttggtcggg
     1801 cgtctctccc aattcgcctg gtggtcacag atcggggcgg cactggagct gaggctcacc
     1861 cgcgaatcga tcgtgctcgg cctcgacggt ggactgacgc ccgaggcgat cctgcaaacc
     1921 ctgacgaggc acagtcaacg cgcgttgccg gccggtgtca ccgacgccgt cacaaactgg
     1981 gccagccgtc gcgagcgcgt gacctactat gccgctgcca ccttgatcga gttcggatca
     2041 tcttccgagc gcgatctcgc cctcgccaac tggcccgtga ccgttctcgc cgcgcctatt
     2101 gcggtagccg agagattcct cctggtcgag gacgagaaaa cggtgccgtt cgaccggctg
     2161 cgcttgatta gctcgcgcga ttatcgccgg cctccagaga tttgtgtgct cgtccagccc
     2221 gacggtgtga cgatgacact cgatccgtct cgctccgatc tacttgttga ggccgaactg
     2281 gcccggttcg ccgaagttct ttctgcgccg caaccggagc catcgcaaat ggcccccacc
     2341 gtgctccgac gtttcgcggt caccgccgcg tcgttgcgcc gggcaaccaa ccggggcatg
     2401 acgtcccccc aactcatcga gtggtattcg cggcgcaccg gcggagagat ccccccggcc
     2461 gtgcgactgt tgctcgcggc tagaacgtcg cgcatgccca tcctgaaggc agcgcgcatg
     2521 ctcatcctcg acctaccgac ggccgagctc ctggatggct tgctgcagca tcccgccacg
     2581 agcccctggc tgggggaccg gctgggcccg accgctgtgg cgatcgccga cgatcggctg
     2641 gcacctcttc aaaaggcctt gaaagatttg ggcattgagt tgcaggctct ttagaggtcc
     2701 cggcccgatt gcaattgagc cggcacgcac caacctgccg gaaagacaga cgctacacgg
     2761 acattcgagc ccggggcgga cttcgaggga agctgggcga acgatcttgt cgagctgctt
     2821 agcaacgtga ccgcacaaag aaccatttcc agcagaagtg gacggagccc gaaagcgcga
     2881 tcgtggatca ttgacgtcat ctttcggcag atggcgcagc ttcaagtgcc ggcttcgcct
     2941 tgacgcgccg ccatcgggag cccgaagtcg cgccctgcgg tgtcgcgggc ttcgagtctg
     3001 cctggtcgcc ggtgccctct tgtgtttgcg cgtcgccgga gggcggacca aaatggtcat
     3061 gatcggccga cgggtccaag tttaagtcgg tcggctcagc ttttgttttg accgaggccg
     3121 gcgcgcccgg aacg
//
LOCUS       DATAQL010000547         5001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4786435, whole genome shotgun sequence.
ACCESSION   DATAQL010000547 DATAQL010000000
VERSION     DATAQL010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4786435"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VHS97_09445"
     CDS             complement(<1..396)
                     /locus_tag="VHS97_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3448465.1"
                     /translation="MTQACRDTQAEPSGHFLDELRATIASRANQEAVTFKDRSFTYGD
                     LDGWAQCWARSLRESGVEPGDRVAIATPEKQVFLAAHLGALYAAAVSLPLNPRAHSEP
                     AIGSDAPCLMLYSSGTTGQPKGVVHTHANM"
     gene            complement(506..3781)
                     /locus_tag="VHS97_09450"
     CDS             complement(506..3781)
                     /locus_tag="VHS97_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX3448466.1"
                     /translation="MVNRLYQCDPDRLRSDLAGELSPGEQAELAGHLEHCGTCQKQLE
                     RLAAGSRIWAELPHLGGPADPSNNGLPPDERTSRPWGKPKPAVGREALAFLAPSENPA
                     YLGRLGPYEVIGVLGEGGMGVVLKAFDPSLSRVVAIKVLAPHMAASGAARRRFSREAK
                     AAAAVVHDHVIAIYAVDTDPESSLPYLVMPCIAGQSLQERIDRDGPLRIEEILRIGMQ
                     TALGLAAAHAQGLVHRDIKPSNILLENGVERVKITDFGLARAIDDASQSQSGVVAGTP
                     QYMSPEQANGETVDRRADLFSLGSVLYAMCAGHSPFRAKTMMGVLRRVCDESPRPLFE
                     INPEVPEALEAAIARLHAKDPAMRYQSASEVAEVLSRQLAELQRPGRRPTSRAIEAGI
                     QPVVTAKPATKKAEAVDDLGSARARPRITKLAAAALLALLGLAVVGTSARFVSPGPPP
                     ARAGIAANLVGQPPDGQVRPPARVTVVSDDNREEIVGSGKPATKAFNLVDFKSVEIMH
                     AFRAEITRADRYAVSVTADDNVLAHVQVANETGKLRVSLEDGRSYRLRRDSLKVAITM
                     PALESLTLTHGARAAISGFESKQPFEARLRHGSLLEGTIGAGRVDFDLMHGSEVVLKG
                     TALTAKLDAQHGSQFLLEGLAIRDAEIHLQHGSKATITARSETDLKAAIYHGSTLNGV
                     IHQGKIGLVAAHGSHVTLSGSAQNATIAGEHSSRFSLGELALETAEVRLDHGSSATVN
                     AKSQLKYNIGHASNLNYLGSPAVSGSVSPHGGSVRPVRAGGDRPTEGKARADEPARAV
                     GKGQNPSEASSPSPRFRGDGHEIITINLGGGRFGSGPLIVGSGQRVTKVVDTKDFTAI
                     RIERLIAADVTRAESFNVTLTADDNILERLEAVRDGSTLWIRLAEGNYRLPERPRVSI
                     TLPILEEIDVAGAASAAVKGFDSDRPFRARASGASILEGSIRAGDVNFEVSGASTVKL
                     SGSAHGARLLASGASKLELTDWQMNGERLTIEVSGASSTRVGGKARAAVLKATGASRL
                     NLPDLSLEAADVVLTGASQANVRVQSLLNYELSSASRLEYFGEPTIDKAEQERSSSVV
                     HRR"
     gene            complement(3793..4404)
                     /locus_tag="VHS97_09455"
     CDS             complement(3793..4404)
                     /locus_tag="VHS97_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3448467.1"
                     /translation="MSEPLLTRPSLLFRLRNLMDERAWGEFVEIYGPLIRQFAQKRGL
                     QDSDVADVEQEVFRAVAVAIDRYDPDPAKGSFRGWLSTIARNLIVNLVNAQRRQPRGA
                     ADTEIQQLLEAHPAPDSEDSALFDVEYHRRLLHWAAERIRDEFSETAWLAFWRTGVDG
                     QSALEAARALGLSVGTVYQYKSRVVARLRREIERFEDKTTDHF"
BASE COUNT          861 a         1612 c         1557 g          971 t
ORIGIN      
        1 catgttggcg tgcgtgtgga caaccccctt gggctgtccc gtcgtacccg agctgtacag
       61 catcaggcat ggtgcatcgc tgcctatggc gggctcggaa tgcgcacggg gatttaacgg
      121 gagcgaaact gccgccgcgt aaagcgctcc caagtgggcc gccagaaaca cttgtttttc
      181 cggggtcgcg attgccaccc ggtctcccgg ttccacccca gattcacgca acgaccgtgc
      241 ccagcattgc gcccatccgt caaggtctcc atacgtgaaa gagcgatcct tgaaagtgac
      301 cgcctcctgg ttggcacgtg aggcgatcgt cgcccgcaac tcgtcgagaa agtgtccgga
      361 cggttctgcc tgcgtgtccc gacatgcctg ggtcatcgac cgccctccct tgacacttcc
      421 gcccgcgaac agaggctcga tcctgcgatc ttaccaggca tcgatcgcga gcccaatcgg
      481 gtgtcgggtc ggcggcgaga cttctctatc tgcggtggac gaccgaagac gagcgttcct
      541 gctcggcttt gtcaatggtc ggctctccga aatactccag ccgcgatgcg gaactgagtt
      601 cgtagttcag caagctttga acccggacat tcgcttgcga cgcgccggta agaaccacgt
      661 cagcggcctc gagggacagg tcgggcagat tgaggcggct agctccggtg gccttcagca
      721 cggcggcgcg cgcctttccg ccgacccgcg tcgaactggc cccactgact tcgatcgtca
      781 atctctcccc attcatctgc cagtcggtca gctccagttt gcttgcgccg ctggccaaca
      841 gccgggctcc atgcgccgac ccgctcagct tcacggtgct ggccccgctg acttcgaagt
      901 tgacatcccc ggcgcggatc gatccctcca ggatgctggc cccactcgcc cgagcacgga
      961 acggccggtc tgaatcgaag cccttgaccg ctgcagatgc ggcgccggcg acgtcgattt
     1021 cctcgaggat aggcaaggtg atcgacaccc gcggccgctc gggcagtcgg tagttcccct
     1081 ctgcgaggcg tatccaaagg gttgaaccgt cccggaccgc ttcgaggcgt tccagaatgt
     1141 tgtcgtctgc cgtcagggtg acattgaacg attcggcccg cgtcacgtcg gcggcgatca
     1201 acctttcaat tcggatggcg gtgaaatcct tggtgtcgac gaccttggtc actcgctggc
     1261 ccgatcctac gatcaacggg cccgagccga atcggccgcc gccgaggttg atggtgatga
     1321 tctcatgtcc atccccgcga aaccggggtg atggcgagct ggcctccgac ggattctggc
     1381 ctttcccaac cgcgcgggcg ggctcatccg cccgcgcctt tccttcggtc ggccgatcac
     1441 ctcccgcccg gaccggacgt accgaaccac cgtgcggcga aacggaacca ctgacagccg
     1501 gactgcccag gtaattcagg tttgaggcgt gtccgatgtt gtacttcagc tggctcttgg
     1561 cattaacggt cgccgacgac ccgtgatcga gacggacctc cgcggtttcg agggccaact
     1621 ctcccagcga gaaccggctc gaatgttccc cagcgatcgt cgcgttttgg gcggagccgc
     1681 tcaaggtaac gtgggacccg tgcgcggcca ccagaccgat tttcccctgg tgtatcaccc
     1741 cgttcagcgt gctcccgtgg tagattgcgg ccttgagatc ggtctcggat cgggcggtga
     1801 ttgtcgcttt tgagccgtgt tgaaggtgga tctcggcatc acggatagct aacccttcca
     1861 gcagaaactg gctaccgtgt tgcgcatcga gcttggcggt cagggcggtc cctttcagga
     1921 ccacctcgct cccgtgcatg agatcgaagt cgacgcgtcc ggcaccgatc gtaccttcca
     1981 gcaggctgcc gtggcgcagc cttgcctcga atggctgttt cgactcaaac ccgctaatcg
     2041 ctgcgcgggc gccgtgtgtc agagtcagcg actccagggc cggcatggtg atcgccacct
     2101 tgagcgagtc gcgtctcagc cggtagcttc ggccatcctc caggctaacc cgtagcttgc
     2161 cggtttcgtt ggcaacctgg acgtgcgcca acacgttgtc gtcagccgtc acgctcacgg
     2221 catagcggtc ggcgcgagtg atctcggcgc gaaacgcgtg catgatctcg accgacttaa
     2281 aatccacgag attaaaggct ttcgtggcgg gcttacccga gcctacgatc tcctcgcgat
     2341 tgtcgtcgct cacgacggtc acccgcgccg gcggacggac ctgaccgtcg gggggctggc
     2401 cgaccagatt ggccgcgata ccggcccgcg caggcggcgg ccccggcgac acaaaccgag
     2461 cagacgtccc cacgacggcc aatccgagca gggcgagcag agcggccgcg gcgagctttg
     2521 tgattcgggg tcgtgctctg gcggatccca agtcgtccac ggcctccgcc ttcttggtgg
     2581 ccggtttcgc cgtgacgacc ggctgaatgc ccgcctcgat tgcgcggcta gtcgggcggc
     2641 ggcccggccg ctggagctcg gcaagttgcc ggctgagcac ctcggcgact tcagacgccg
     2701 actggtaccg cattgccggg tctttggcat gcaaccgcgc gattgcggcc tccaaggctt
     2761 cgggcacttc cggattgatc tcgaagagcg gccgcggtga ctcgtcacag acccgccgca
     2821 gcacccccat catcgtcttg gcgcggaatg gcgaatgccc ggcgcacatg gcatacaaca
     2881 cgctgcccag gctgaaaagg tcagcccggc ggtcgaccgt ttcgccattc gcctgctcgg
     2941 gcgacatgta ctgcggcgtc ccggctacca caccgctctg tgattggctg gcgtcgtcga
     3001 tggcgcgggc cagcccgaag tcggtgatct tgactcgctc gacaccgttt tccagcagaa
     3061 tgttcgacgg cttgatgtcg cgatggacca ggccctgggc atgtgcagcg gcgagtccca
     3121 gcgccgtctg catgccgatg cgcaagatct cctcgatgcg caacggtccg tcgcggtcga
     3181 tcctttcctg aagcgattga ccagcgatgc agggcatcac caggtagggg agactgctct
     3241 cggggtcggt gtcgacggcg tagatggcga tcacatggtc atgaacgaca gctgcggcgg
     3301 ccttggcttc gcgggagaat cgtctacggg cggcgccgct ggcggccatg tggggcgcga
     3361 ggaccttgat ggccacgacg cgactcaacg acgggtcgaa cgccttgagc accactccca
     3421 tgccgccctc acccagaacg ccgatgacct cataaggccc gagccggccc agatatgccg
     3481 gattctccga aggggcgagg aatgcgagcg cctcgcggcc gacggccggt ttgggcttac
     3541 cccatggccg gctcgtccgc tcatcgggcg ggagtccgtt attggacgga tccgccgggc
     3601 cgcccagatg cggcagttcg gcccagatgc ggctcccggc ggcgagccgt tccagttgct
     3661 tctggcaagt gccgcagtgt tcgaggtgac ctgccaactc ggcttgttcg cccgggctca
     3721 gttcgccggc gaggtcagac cgcaatcgat cgggatcgca ttggtacagc cggttcacca
     3781 tcgcagtgcc ccttagaagt gatctgtagt cttatcctcg aatcgctcga tctctcggcg
     3841 gagccgggca acgactcggc tcttgtattg atacacggtg ccgaccgaca ggcccagggc
     3901 tcgggccgct tccagggcgg actggccatc cacgccggtc ctccagaagg ccagccatgc
     3961 cgtctcggag aactcgtcgc gaatccgttc ggccgcccag tggaggagac ggcgatggta
     4021 ctccacgtcg aagagggcag agtcctccga gtccggcgcg ggatgcgcct cgaggagctg
     4081 ctggatctca gtgtctgccg cgccgcgcgg ctgtcgccgc tgggcgttca cgaggttaac
     4141 gatgaggttg cgggcgatcg tcgagagcca ccctcggaat gatcccttgg ccgggtcggg
     4201 atcatagcga tcgatcgcga cggcaacggc ccgaaaaact tcctgctcga cgtcggccac
     4261 gtcggaatcc tggagcccac gcttttgcgc gaactggcgg atcagcgggc catagatttc
     4321 gacgaactcg ccccaggctc gttcgtccat caagttgcgt agccggaaca acaggctggg
     4381 tcgcgtcagc agcggttcgc tcattcgttg cactccaaga gaatacacaa tcgccggcaa
     4441 ggaatttgaa cggattcgga gatttttcca ccaccggtcc aatgggtttc tgaggtcaat
     4501 cagccacgcc ggcctggacc cggcctcgag cgattccggc gacgatccgt ccacttggga
     4561 cggctgaatc ggttttctgg gcggctagtg cccattcgat gcgcggaaat acgtgattgg
     4621 tttcaatcgc tcaaaagtgt gtcactgccg acatgaactt aggtgacacg ccctaaataa
     4681 ccatggcata aaatgttacg ccgagtcact cgaaagacac cggttgacct tgtgtgtcac
     4741 cgtgtgtcac cgactactgt cacggtgtgt caccgttttt gactgatcgt tgacgttcaa
     4801 gtgcgtgaag ttatcgactt cgatcgctgc ggagcggccc gacgtgcgct cggtctctat
     4861 cgctcgagcg atttggcctg aaaataacga ttcgccccgc cctcgagctt cggtctaatg
     4921 gtaacggatg gaccgaacaa acgcagttgg ggcgcaattc ggagcagctc agttgcggcg
     4981 cgcagatgtc ttcggccagc c
//
LOCUS       DATAQL010000548         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1580258, whole genome shotgun sequence.
ACCESSION   DATAQL010000548 DATAQL010000000
VERSION     DATAQL010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580258"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VHS97_09460"
     CDS             complement(<1..223)
                     /locus_tag="VHS97_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448468.1"
                     /translation="MKRLSLTVVKVGGSLFDWPEFPHRVAAFLHTLEADDSRERIVLI
                     AGGGQAADLIRVLDDLHSLGDETAHRLALL"
     gene            complement(220..1275)
                     /locus_tag="VHS97_09465"
     CDS             complement(220..1275)
                     /locus_tag="VHS97_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX3448469.1"
                     /translation="MSNLIEQRLPWIGLDVGGANIKAAHEDGPARTVPFEVWKRPDEL
                     SRAIAAAVAAFPPSDRAAVTMTAELCDCYPTKALGVSAVLDAVLEALPGWLIVVWGVD
                     GAFHSVAEARERPHIVAAANWLALAYLAARLIPAARGLLIDIGTTTTDLIPLDRGKVA
                     ARGRSDTERLQTGELVYAGVRRTPVCALTTELPVRGIPTGLAAEIFASTLDVYLTLGD
                     IESNPTDLSTADGRPATALAARDRLARMVGTDRDGFSSQDALEFAQAADQCLLDRLAH
                     AARMAAASTIGRPTAAVVAGSGEFLARRLAHKLIEPDGPIVSLKEAWGEVASSAGCAH
                     ALVKLAAERYRNTDDPI"
     gene            complement(1272..2369)
                     /locus_tag="VHS97_09470"
     CDS             complement(1272..2369)
                     /locus_tag="VHS97_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX3448470.1"
                     /translation="MIWKGDCDIPNQPPRTVLIHEWVTGGGLSGSTLPASWAAEGRAM
                     RRSIAADFAALPKSSVRVVVTLDVRLPHDPGPWQTEPIAPGNYADRLRALARAADFTV
                     LIAPETSGILAGLTRDLTAAGARLLGSTALAVELAADKACTAARLEELSISTPPTRTI
                     VPSAGLPSDTEYPAVLKPVDGAGSLDTFYLSDALSLPPAALAMPRAVLQPFVPGTPMS
                     ASFLVGPAGKAWLIGVGIQRVAVRDGRFQYLGGTLPAACPEAVPQLTKAVEAFAGLHG
                     FVGVDFIWNEARQHAMILEINPRPTTSYVGIGRLLPAGLLARAWLTACEALEWELETL
                     AGLAENVHAKPVLSFQADGELIDRDSGAFRS"
     gene            2344..3111
                     /locus_tag="VHS97_09475"
     CDS             2344..3111
                     /locus_tag="VHS97_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX3448471.1"
                     /translation="MSQSPFQIIPVIDLLNGQVVHAVGGRRAYYQPIQSKLHATSEPI
                     PIARAIRAALGLPTLYFADLDAIAGAPPRLDIYCELIARRFHLWVDAGVRDAETVAPL
                     LELDPAFTTIIAGLETLRGPRELTKIVQMAGVDRIVFSLDLFEKRPRMTAAKAWGTED
                     SLELAQLAIDCGVHQLLILDLARVGTGRGLGSHDLIARIRDARPSVQLTAGGGISRIE
                     EVLDLRDVGANGVLIGSALHDGRIGIRELEQIAAIGH"
     gene            complement(3119..3628)
                     /locus_tag="VHS97_09480"
     CDS             complement(3119..3628)
                     /locus_tag="VHS97_09480"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX3448472.1"
                     /translation="MSSSLFKVRVTKDYLVFCSGHFITYDGDHCERMHGHNYRATVEV
                     EGDLDHNHYVIDFIALKDLTRAITDELDHRMLLPTQSRQIQLHEDGPNLRVTYRDRYW
                     SFPRDECALLPIANTTAELLADYIAGRLRQAMTARGWVLPRILRVEVEESFGQAAQVE
                     WHRDFDTSR"
     gene            complement(3697..>4120)
                     /locus_tag="VHS97_09485"
     CDS             complement(3697..>4120)
                     /locus_tag="VHS97_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEX3448473.1"
                     /translation="RDSIARQYANGYQQVFGEALPALRASLDAGLPLETAIIFSYLKL
                     LARYQDSLIARKYGQDWAHEVSHRAAAILDAGWPVEREAHRMFETFDSWLRHPANRFN
                     PGTTADLMTAALYAALRDGIVKLVGTTAIWEMPNAEAP"
BASE COUNT          781 a         1287 c         1295 g          757 t
ORIGIN      
        1 ccaagagggc cagtcggtgt gcggtctcat cgcccaggct gtgaagatcg tcgagcacgc
       61 gtatcagatc ggcggcttgg ccgccgccgg cgatcagcac gatccgctct ctgctgtcat
      121 cggcctcgag cgtgtggaga aacgcggcca cgcgatgagg gaattcgggc cagtcaaaga
      181 ggcttccccc caccttgacc actgtgagag ataggcgctt caaatcgggt cgtccgtatt
      241 tcgataccgc tccgccgcga gtttgaccaa agcatgagca cacccggccg acgaagcgac
      301 ttccccccag gcttctttca aactgacgat cggaccgtcg ggctcgatca gcttatgcgc
      361 caatcgccgc gccagaaact ccccagatcc ggcgacgacc gcggcagtcg ggcggccgat
      421 cgtggacgcc gcggccatgc gggctgcgtg ggccagccga tcgagcaagc attggtcggc
      481 ggcctgcgcg aactccagtg cgtcctggga agagaatccg tcgcggtcgg tgccaaccat
      541 tcgtgccagg cgatcgcgag ccgcaagggc cgtggcgggc cgaccgtcgg ctgtcgagag
      601 atcggttggg ttggattcaa tgtctccgag cgtgagatag acgtcgagcg tcgaggcgaa
      661 gatctcggcg gccaggccgg tgggtatacc tcgtacaggg agctcggtcg tcagcgcgca
      721 cactggcgtc cggcgtactc cggcgtagac cagctcgccg gtctggaggc gctcggtgtc
      781 gctacgaccg cgtgctgcca cttttccccg atcaagcgga atcaaatccg tcgtggtcgt
      841 gccgatgtcg atcaagagcc cgcgcgctgc tggtatcaga cgggcagcca ggtacgccag
      901 cgcaagccag ttggctgccg ccacgatgtg gggccgctct ctggcctcgg cgaccgagtg
      961 aaacgcgccg tcaacgcccc agacaactat taaccagccc ggaagtgctt cgagcaccgc
     1021 atcgaggacc gcgctcacac ccagcgcctt ggttggataa caatcgcaaa gctccgccgt
     1081 catggttacg gcggcccgat cgctgggagg aaaagcggcg actgccgccg cgatggctcg
     1141 actcagctcg tccgggcgct tccaaacttc aaagggaacg gtccgagccg gtccgtcctc
     1201 gtgcgcggcc ttgatgttgg ccccgccaac gtccaggccg atccaaggta aacgttgctc
     1261 tatcaaattg ctcatgagcg aaaagctccg ctgtcacgat caatcagctc gccgtcggcc
     1321 tgaaacgaga ggaccggctt tgcgtggaca ttttcggcca gtcccgcaag cgtttccagt
     1381 tcccattcta aggcttcgca agccgtcagc cacgcgcgtg ccagcaagcc ggccggcagc
     1441 aggcggccaa tgcccacgta cgaagtcgtt ggtcgcgggt tgatttcgag gatcatggca
     1501 tgttgcctcg cctcgttcca gatgaaatcc acaccgacga acccgtggag accggcaaat
     1561 gcctcgaccg ccttcgtgag ctgcggaacc gcttcagggc acgctgcggg caacgtgcct
     1621 cccaggtatt gaaaccggcc gtcgcgtacc gcaactcgct ggattcccac gccgatcagc
     1681 caggccttgc cagccgggcc taccaggaaa ctcgcactca ttggtgttcc cggtacgaat
     1741 ggctgaagca cggcacgcgg cattgccaac gccgccggcg gcaggctgag tgcgtcggac
     1801 aggtagaacg tatcgagtga gccggcgccg tcgaccggct tgagaactgc cggatattcc
     1861 gtatcgctcg gcaggcccgc actggggact atcgtcctgg tcggcggcgt gctgatcgac
     1921 agttcttcga gacgcgcggc agtgcacgct ttatccgcgg ccagctcgac cgctaaggcg
     1981 gtcgagccca gtaaccgcgc gccggccgcg gtgagatcgc gggtcaagcc tgccaggatt
     2041 ccactcgtct ccggcgcgat cagcacggta aaatcagccg cccgggcgag cgcacgcaga
     2101 cgatctgcgt agtttccggg ggcgattggc tcggtttgcc aggggccggg atcgtgtggt
     2161 agtcgtacgt ccaatgtcac gacgacgcgt accgagctct tgggcaacgc ggcaaagtcg
     2221 gctgcgatcg atcgccgcat ggcgcgaccc tcggccgccc acgaggcggg caaagttgag
     2281 ccggacagcc ccccgcccgt gacccattca tggatcagga cagtccgggg aggttggttg
     2341 ggaatgtcgc aatccccctt ccagatcatt ccggtaatcg acctcttgaa cggccaggtg
     2401 gtccacgcag tcggcgggcg gcgagcctat tatcaaccga ttcagagtaa gttgcacgcg
     2461 acttcggaac cgattccgat cgcccgagcc atccgcgctg ccctcggctt acccacgctt
     2521 tattttgcgg acctggacgc gattgcgggc gcgccgccac gactagacat ctactgtgag
     2581 ctcatcgcca ggcgatttca tctctgggta gatgccggag tccgcgatgc cgagacggtg
     2641 gcacccttgc tcgagcttga tcctgccttc acaacgatca tcgccgggct tgagaccctg
     2701 agaggccctc gcgagctgac caagatcgtg cagatggcgg gggtggatcg gatcgtgttc
     2761 agtctcgact tgttcgagaa acgccccagg atgaccgcgg cgaaggcctg gggaaccgag
     2821 gattctttgg agctggctca gttggcgatt gattgcggcg tgcaccaact gctgattctg
     2881 gatctggcgc gcgtaggaac cggccgtgga ttgggcagcc acgacctgat cgctcggatt
     2941 cgcgacgccc ggccgtcggt gcagctcacc gccgggggcg gaatctctcg tatcgaggaa
     3001 gtcttagatc tccgcgatgt cggtgcaaac ggggtcttga tcggctcggc cctccacgac
     3061 gggcggatcg gaattcggga actcgagcaa atcgctgcaa tcggccactg acgggtcatc
     3121 atcttgacgt gtcgaaatct cggtgccatt ccacttgcgc cgcctggcca aagctctcct
     3181 caacctcgac gcgcaaaatg cgtggcagaa cccaaccgcg cgcggtcatc gcctggcgaa
     3241 gccgcccggc gatgtagtcc gccagcagct cggccgtggt gttggcgatc ggcaataagg
     3301 cgcattcatc gcgcggaaag ctccagtaac ggtcgcggta ggtgacgcgc aaattcgggc
     3361 catcttcgtg caactggatc tgccggctct gggtcggcag cagcatgcgg tgatcgagtt
     3421 cgtcggtgat cgcgcgggtc agatccttga gagcgatgaa gtcgatcacg tagtggttat
     3481 ggtcgagatc tccctcgacc tcaacggtcg cgcggtaatt gtggccatgc atccgctcgc
     3541 agtggtcgcc gtcgtacgtg atgaaatgcc ccgagcaaaa gaccaggtaa tccttggtaa
     3601 cgcgaacctt gaaaagtgat gacgacatgg ctggcaaatc catcggcgaa cgacaattac
     3661 gaacattaac gcatatcgat gtgaacgcaa gagaagttac ggggcttccg catttggcat
     3721 ctcccaaatt gcggtcgtcc ctacgagttt cactatccca tctcgtaatg ccgcgtacaa
     3781 ggcggccgtc atcaagtcgg ccgttgtgcc gggattgaat cgattggccg ggtgccggag
     3841 ccaggagtca aacgtttcaa acatgcgatg tgcctcgcgc tctacgggcc agccggcgtc
     3901 caggatcgcg gccgcacggt gcgagacttc atgtgcccaa tcctgtccat acttgcgcgc
     3961 gatcaaagag tcctggtacc gcgccaggag cttcaagtaa gagaagatta tcgcggtctc
     4021 gagcggcaag ccggcatcga gcgatgcgcg gagtgccggc aacgcctccc cgaaaacctg
     4081 ctgatacccg ttggcgtact gccgcgcgat tgaatctcgc
//
LOCUS       DATAQL010000549         5948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2802633, whole genome shotgun sequence.
ACCESSION   DATAQL010000549 DATAQL010000000
VERSION     DATAQL010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2802633"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..2786
                     /locus_tag="VHS97_09490"
     CDS             519..2786
                     /locus_tag="VHS97_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3448474.1"
                     /translation="MDLFADLTAAQRDAVSHVDGPLLVLAGAGSGKTRVITRRVAHLL
                     STGISSDRILALTFTNKAAGEMRERIDALVPNSRVLVGTFHGFCARMLRKYSPLVGID
                     TSFSIYDQNDRLKAVKDVMEQLGWNEPGWTGERVESAISRAKNDLLSPAALKHRAFDE
                     TGRRVAQVYEIYEQRLRFASAVDFDDLLVHLVAILKNHHDVRADLDKRFRYVLVDEYQ
                     DTNLAQYAIVRALSVDYPNICVTGDPDQSIYGWRGANLANILEFEKDFPGCHVVTLER
                     NYRSTKNIISVADHLIRYNRNRKAKALRTENPIGEPVELTIYARETDEAEGVAGRISS
                     LVREGEYRFADIAVFCRVTALTRPIEQAFRGARIPYQVVGGVAFYERQEVKDVLAYLN
                     LIVNPKDDLAFARVVNVPLRGIGKTSVEHLAAAARERGIPLLAMARQAGTVPALRGKA
                     ARGLEEFTRLIDELTAWRDRPAEEVVAELLKRTNYREHLAAEKRDNGNDRSANLDEVI
                     TAAREFDQAHPGSSIQDFLAEITLASAIDRWEQDSGAVTLMTLHAAKGLEFPVVFIVA
                     LENGLLPHARAGENENQEEEERRLFFVGITRAQRELYLSRCVVRTFRGQQQATFSSKF
                     LTELPEGPIVVRDFSGVGGRSDLSPAMAGGRRPPFGYDQRPRSAPGEFRLTTAAALAG
                     SLGEIVPGGAHAQINLDAFQIGVLVVHPEYGLGRITATEGEGTGRKGKVAFAVGPART
                     FVFAKSPLKPMSKPT"
     gene            2877..3623
                     /locus_tag="VHS97_09495"
     CDS             2877..3623
                     /locus_tag="VHS97_09495"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX3448475.1"
                     /translation="MPRLGVNIDHVATLREVRGGREPDPVWAAVLAELGGADVITIHL
                     REDRRHIQDRDLRLLRQTVQVRLNMELAVEPKIIALALEIKPDQATLVPERREELTTE
                     GGLDVVGQRDQVQGALSQFHDSGIDVSLFIDPDPKQVEAAVALGAAAVEFHTGRYANA
                     QVGPDRTRELDALKAAAVTAVTSGLKLHAGHGLNYQNVSPVARLDRMAELNIGHSIIS
                     RAVFVGMTLAVREMKECIDRAVGAAALPGG"
     gene            3966..4865
                     /gene="dapA"
                     /locus_tag="VHS97_09500"
     CDS             3966..4865
                     /gene="dapA"
                     /locus_tag="VHS97_09500"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX3448476.1"
                     /translation="MPRKGQMFSGCTVALVTPFKDGAIDEPALRRLVDWQIDQGTPIL
                     SPVGTTGEAPTLTHDEHERVIDIVVEAAAGRCKVMAGTGSNSTAEAIRLTRFAARAGA
                     DGALLVAPYYNRPNQEGMYRHFAAVCDSVDLPVVLYNIPARTGRNVEPDTIARLTSIG
                     QIVAVKEASGSLDQVSELLVRTDLTVLSGDDSLTLPMLAVGAEGVVSVVANLVPRDVM
                     ALVEAFTSGRTDLAREWHARLFPLCRDLLGLAPNPIPVKSALAVLGRGNGELRLPMCA
                     LDDAARKSLNKSLITYGLLTDRS"
     gene            5255..5668
                     /locus_tag="VHS97_09505"
     CDS             5255..5668
                     /locus_tag="VHS97_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3448477.1"
                     /translation="MAQKVAKRHAETNGQSSTAAPEVSDQSVRELAQVFKLLSDETRL
                     RILFYLALSPNGELHVTDLCQRLGQSQPAVSHHLALLRVSGLIESRREGKHNFYCVRT
                     EHFGELLLSLFSAAGEVPRNKKYRFHDFVLAYSGE"
BASE COUNT         1173 a         1772 c         1754 g         1249 t
ORIGIN      
        1 gggcctggca gtagcggatc tcccgctccg tgtagacgcg ccgcagaaac agctcgccat
       61 ggtgctcgat catcttgcct atgcgcgggc actcgatgat atcggtgccg attcccaaga
      121 tttccactcc tgtccccttc ccttggccga gccggtcgaa ttgacctcgt gataggcaga
      181 tcgccaatgt tcagtgcggc atggcgttcc aggccgcggt tcaccacgtg caagatgccc
      241 gtgccacacc acactgcggg ctgacgcagt gcgagtcttg acccaatcta attccgaacg
      301 actcaacggc caagtgctca atcaatgacg actcttcaat attcggccct cttcgcactc
      361 tatcgacgag ctacgcagga ggaaggtcgc gcggcggccg aagtcgtgtc gcggcccatg
      421 gcctgcgccg ctatactacc gacggcgata gaattcgcga cgaacccgga gacaacgccc
      481 gggaagctgg gccggagtag cccgaggaag accccttgat ggatttgttt gccgatctga
      541 cggccgctca gcgcgatgcc gtgagccatg tcgacggtcc gctgttggta ctggcaggcg
      601 ctggctctgg caagacccgt gtgatcaccc gccgcgtcgc tcacctcctc tccacgggaa
      661 tttccagcga ccggatcctg gccttaactt ttaccaacaa ggcggccggc gagatgcgcg
      721 agcggatcga cgccttggtt cccaactcgc gcgtcttggt cgggacattt cacggctttt
      781 gtgcccgtat gctccgcaag tactctccct tggtcggaat cgacacgagc ttttcgatct
      841 acgaccagaa cgaccggttg aaagccgtca aggatgtaat ggaacagctc ggctggaacg
      901 agccgggctg gaccggcgaa cgggtcgagt cggcgatcag ccgcgccaag aacgaccttt
      961 tgagcccggc agccttgaag caccgtgctt ttgacgagac cggtcgcagg gtggcccagg
     1021 tttatgagat ttacgaacag cgcctgcgct ttgcttcggc agtcgacttc gacgacctct
     1081 tggtccactt ggtcgcgatc ctcaagaatc accacgacgt tcgcgccgat ctcgacaagc
     1141 gattccgata tgtgctggtt gatgaatatc aagatacaaa cttggcacaa tacgcgatcg
     1201 tccgtgcgct ctctgtcgat tatcccaaca tctgcgtcac cggcgacccc gatcaatcga
     1261 tctacggttg gcgcggcgct aatctggcga acattctcga gttcgaaaaa gacttcccgg
     1321 gctgccatgt cgtcacgctc gagcgcaatt accgcagcac gaaaaacatc atcagcgtgg
     1381 ccgatcacct gatccgttac aaccgtaacc gcaaggccaa ggcgttgcga acggaaaacc
     1441 caatcggtga gcctgtcgag ctgacgatct atgcgcgcga gaccgacgag gccgaagggg
     1501 tcgcgggaag gatctcgtcg ctggtgcgcg aaggggagta caggttcgcc gacatcgcag
     1561 tcttctgtcg cgtgacggcc ctcacgcgac cgattgaaca ggcctttcgt ggcgctcgca
     1621 taccttatca ggtcgtgggg ggcgtcgcgt tttatgagcg ccaggaggtc aaggacgtac
     1681 tggcctactt gaatttgatc gtcaacccca aggacgacct ggcgttcgcg cgggtggtca
     1741 acgttcccct gcgcggcatc ggcaagacct cggtcgaaca tctggccgcc gcggcgcgcg
     1801 agcggggtat cccgcttctg gcgatggcgc ggcaagctgg tacagtgccc gcgctcaggg
     1861 gcaaagccgc gcgcggcctg gaagagttta cccggctgat tgacgagctc accgcctggc
     1921 gtgaccgacc tgccgaagag gtcgttgccg aactcttgaa aagaaccaat tatcgcgagc
     1981 acctcgcggc cgagaagcgc gataacggaa acgaccgttc ggcaaacctc gacgaggtga
     2041 tcacggccgc ccgcgaattc gatcaagcgc accccggctc ttcgattcaa gacttcttag
     2101 ccgagatcac cctggcttcg gcgatcgacc gctgggagca ggactcgggc gccgtcacct
     2161 tgatgacgtt gcacgcggcc aagggcctgg agtttccggt cgttttcatc gtcgcgctcg
     2221 aaaatggtct gttgccgcac gctcgcgccg gcgaaaacga aaaccaagag gaagaggaac
     2281 ggcggttgtt ctttgtcggc atcacccgag cgcagcgcga gctatatctc tcgcgatgcg
     2341 tggtaaggac gtttcgaggt cagcagcagg cgacattctc atcgaagttt ctcaccgagt
     2401 tgccggaggg accgatcgtt gtccgcgatt tctcgggggt ggggggccga tccgacctgt
     2461 cgccggccat ggccggcggg cgccggccgc cgtttggcta cgaccaacgc ccgcggtcgg
     2521 caccgggtga attccggctg accacagccg cagcactcgc cggctcactt ggggaaatcg
     2581 ttcccggcgg agcccacgcc caaattaacc tcgatgcctt ccagatcggt gtcctggtcg
     2641 ttcaccctga gtatggattg ggacgaatca cggcgaccga aggagaagga actggtcgca
     2701 agggaaaagt cgcgtttgcg gtaggtcctg cccgaacatt tgtgtttgcc aagtcgcctt
     2761 taaagccgat gagtaagcca acataaaata actatccccc cacgcgatac cggcctatag
     2821 ttgccggggt tggattagcg tgagactggc taattgcccc ctggagaaca acgagcatgc
     2881 cgcgtttggg tgtcaatatt gatcatgttg ccacgcttcg tgaagtgcgt ggcggccgtg
     2941 agcccgaccc cgtatgggcg gcggtactgg ccgagctcgg cggggcggac gtgatcacga
     3001 tccacctccg cgaagaccga cgacacatcc aggatcgcga tttgcgcctg ctgcggcaga
     3061 cagtccaggt ccggctcaac atggagctgg cagtcgagcc gaaaatcatt gccctggccc
     3121 tggaaatcaa gcccgatcaa gccacacttg tgcccgagcg gcgcgaggag ctgacgaccg
     3181 aaggaggctt ggacgtggtc ggccagcgcg atcaagtgca gggcgccttg tcccagtttc
     3241 acgactcggg catcgacgtg tcgcttttta tcgacccgga tcccaaacaa gtcgaggccg
     3301 cggttgcgtt gggcgccgcc gccgtcgagt ttcacaccgg acggtacgcc aacgcacaag
     3361 tagggccgga ccgcacccgc gagctagacg ccctaaaagc cgccgcagtc acggcggtta
     3421 cctccggcct gaaactgcat gctgggcacg gtctcaatta tcagaacgta tcaccggtcg
     3481 cgcgcctgga ccggatggca gagctcaata tcggccatag tatcatcagc cgcgcggtgt
     3541 tcgtcggtat gaccctggct gtccgtgaga tgaaagagtg tattgatcgg gccgtcggtg
     3601 cggcggcctt gcccgggggg tgaatatccc tggaatcagg aatcccttat gcatgccatc
     3661 actaatgatt gccgacgtct ttccgcccgc gtgtgacagg tgtaccagca tcaagaaagg
     3721 ggttttttgc gtttttcggc caagataaat ctctgaccgc ttcactttcg gagggaacca
     3781 ctataagatt cgttatcgtt caacccatgg tgctgcgaat attatatcgc aagactactc
     3841 gaacctggca tctatcgtgc tagctgctcg ggattgcttc gagcctgcct gtttgtggtt
     3901 gatccgaggt tgccggctga tggtgtcgaa cgcgtttcgc cgcaactgtc ttctcagaga
     3961 atcgcatgcc tagaaaaggt cagatgttct ctggttgcac ggtcgcgctg gtcaccccgt
     4021 tcaaggatgg ggcgatcgac gagccggcac tacggcgact tgttgattgg cagatcgacc
     4081 agggcacgcc gatcttgagc ccggtgggaa ccacgggcga agctcccacg ctcacacatg
     4141 acgagcacga acgtgtgatt gatattgtgg tagaggctgc ggcgggccgc tgcaaagtca
     4201 tggccggcac gggctcgaac tccacggctg aggcgatccg actgacgcga tttgccgcac
     4261 gagccggggc cgacggcgcc ctgcttgtcg ctccttacta taatcggccc aatcaagaag
     4321 ggatgtaccg gcacttcgcg gccgtttgcg acagtgttga tttgccggtc gttctttaca
     4381 acatcccggc gcggaccggc cgcaatgtcg agcccgatac gatcgcacgg ctcacctcga
     4441 tcggccagat cgtggccgtc aaagaggcct ccggctcact cgaccaggtt agtgaactcc
     4501 tcgttcgcac ggatctcacc gtactctcgg gagacgacag tttaacgctt ccgatgctgg
     4561 ccgtgggcgc cgaaggagtg gtttcggtgg tcgccaacct cgttcctcgc gacgtgatgg
     4621 cactcgtcga ggcctttacg agtggccgca cggatctcgc gcgcgagtgg cacgcccggt
     4681 tgtttccgct ctgccgggat ttgctggggc tggctcccaa cccgattccc gtaaaatcgg
     4741 ccctggctgt gctgggccgg ggaaatggcg agctgcggtt gccaatgtgt gcgcttgacg
     4801 acgcagcgcg taaatcgttg aataagagcc tcataactta tggtctcttg actgatcgct
     4861 cataatgact acatgaccat aatgcgcgac aagttgtgtt gtgattccgc cgcatatgcc
     4921 tccttgcatt gatctaagac ggagattcca gatttgagta attgacctcg acggatcgat
     4981 cggcaggaag acatgtggcc aactgacccg aacgcgtgtt cggttgcgat aggtttgacg
     5041 gttcctccgc ttttccccac gaaattcgct cgccaattga aagatgcgga ttgtcgcatt
     5101 gcacccaact ttgcgcgccg gatcggtcac ccgttttcgt tgtcgtttca gtgggtgatt
     5161 ccgcattaaa accgcaagcg aaattggagt gtttgaagcc gacttggagt cggtttctcg
     5221 attttcttca caacatagtt gaggatctct acccatggca caaaaagtag ccaagcgtca
     5281 cgccgagacc aacggccaat cgagtacggc agcgcccgag gtatcggatc aatctgtccg
     5341 cgagcttgct caggtgttca agctcttgag cgatgaaacc cggttgcgca tcctgtttta
     5401 cctcgcctta tcccctaacg gcgagctcca cgtcaccgac ttgtgccaga gactcggcca
     5461 gagtcagccc gcggtcagcc atcacctggc gctcctgcgc gtttccgggc tgatcgagtc
     5521 acgtcgtgaa ggcaagcaca atttttattg cgtgcggacc gaacacttcg gcgagctgct
     5581 cctctcgctg ttctcagcgg ccggcgaggt tccccgcaac aagaaatatc gctttcacga
     5641 tttcgtcttg gcctactccg gagagtgaaa atcggagttc ccacagcctg aatatcgtcc
     5701 cgcttttttt ccctttggga atcccgtggc ttctccggta agagctgggc tgtcacggga
     5761 ttccgttgtt tgtatcgctc tacaaccgac acttgatgct tgcatgcatt tggcatcgac
     5821 cgtcccgcga atatacggga ggtctcggct tgcgcggtcg gttagagcgt tgtaaacgcg
     5881 ccagttcggc ttgacccatc cacttcctga gcgcaaaatg ccgcgcgttg ccatccggcg
     5941 aacacttt
//
LOCUS       DATAQL010000550         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_816420, whole genome shotgun sequence.
ACCESSION   DATAQL010000550 DATAQL010000000
VERSION     DATAQL010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816420"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VHS97_09510"
     CDS             complement(<1..384)
                     /locus_tag="VHS97_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3448478.1"
                     /translation="MSINWDSLRATEFPVADHWAYFDHAAVAPLPRRSAEALRGFAHE
                     QLVDGVVGWPAREQRIEAIRDQVALLINAHRDEIAFIPSTTHGIGLIAEGFPWREGDN
                     VVSAAEEYPSNLYAWMNLESRGVKLR"
     gene            complement(428..649)
                     /locus_tag="VHS97_09515"
     CDS             complement(428..649)
                     /locus_tag="VHS97_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448479.1"
                     /translation="MKKLSNRWKRFVAPVVLGLGLCVAPAFSPPARAQAQEETADPNA
                     EKGRPFDGYIATISLMLLALFIVGKSARR"
     gene            complement(646..1206)
                     /locus_tag="VHS97_09520"
     CDS             complement(646..1206)
                     /locus_tag="VHS97_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HEX3448480.1"
                     /translation="MAQRSNHYEAAFESFIRSIRVPCVAIDEAKRAICGEDGVKNPDF
                     LLYPQFGVNLVVEVKGKRAKNKRGRRDWENWVTTDDLDGLTRWQAMFGPGFRAVLAFV
                     YAEPIQPFPLPGNNGAYEFRGLEYRFWAVGLDDYIAVLRSRGPAWKAVAMARRAFRRR
                     VRPLLEWLPMSYDWTRRPIPVKERQR"
     gene            complement(1321..1788)
                     /locus_tag="VHS97_09525"
     CDS             complement(1321..1788)
                     /locus_tag="VHS97_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX3448481.1"
                     /translation="MAATRAKGRKPKREQLKPGECLCDFCTGACCRYFSLPITNPTTW
                     DEYDAIRWYLAHGQTVIYVEKGQWYLLVMTRCQYLTADNRCGIYLNRPKICREYTTDN
                     CEYDNSWSFEKIFETPEQIWEYAEAVLPPRRKRRDPAAISLPIVTLGGPKSGS"
     gene            complement(1769..>1985)
                     /locus_tag="VHS97_09530"
     CDS             complement(1769..>1985)
                     /locus_tag="VHS97_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448482.1"
                     /translation="ESVPRPRERTLSQPDEKHVIEPNRTHGRGDVTAVRRTCLTDGGM
                     FRLTPASRLSNTVRPCTETSLNGRDQS"
BASE COUNT          386 a          600 c          617 g          382 t
ORIGIN      
        1 ccgcagcttg acgccccggc tttccaggtt catccaggcg taaaggttcg agggatattc
       61 ctcggcggcc gagacgacgt tgtctccctc gcgccaggga aacccctcgg cgatcaggcc
      121 aatgccgtgg gtcgtgctgg ggataaaggc gatttcgtca cggtgtgcat tgatgagcag
      181 ggcgacctgg tcgcggatgg cctcgatccg ctgttcgcgg gcgggccagc ccacgacgcc
      241 atctacaagt tgctcgtggg cgaagccgcg gagggcctcg gcggaacgcc ggggcagagg
      301 ggccaccgcg gcgtggtcga aataggccca gtggtccgcg accgggaact cggttgctcg
      361 cagggaatcc cagttaatac tcatgaagat gcgctcgccc gcctagccca gcgtcccggt
      421 cagacggtca gcgcctggca gacttaccca cgatgaaaag ggccagcaac atcaacgaga
      481 tggtggcgat atagccgtcg aatggacggc ccttttcggc gttgggatcg gcggtctctt
      541 cctgcgcctg cgcgcgggca ggagggctga aggccggcgc cacgcacagg cccagcccca
      601 aaacgaccgg ggcgacaaag cgcttccatc gattcgacag tttcttcatc gttggcgttc
      661 cttgacggga atgggacgac gagtccaatc ataactcatg ggaagccatt cgagtaaggg
      721 gcgaacgcgg cggcggaaag cgcggcgagc catggcgacc gctttccacg cgggcccgcg
      781 cgaccgcaga acagcgatgt agtcatcgag accgacagcc cagaaacgat actcgagccc
      841 gcgaaactca tacgccccat tgttgcccgg caggggaaat ggctggatcg gctcggcata
      901 gacaaatgcc aggaccgcgc gaaaaccagg tccaaacatc gcctgccagc gtgtcaggcc
      961 gtcgagatcg tcggtcgtca cccagttttc ccaatcgcgc cgtccgcgct tgttcttggc
     1021 acgcttacct ttgacctcga cgacgagatt aaccccaaat tggggataga gcagaaaatc
     1081 cggattcttg accccgtcct cgccgcagat ggcgcgcttg gcttcgtcga ttgcgacgca
     1141 cgggacccgg atcgagcgga tgaacgactc gaaggccgcc tcgtaatggt tggatcgttg
     1201 ggccatgagc gcgcttttcc gtggatcact cctcgatcgc tgccgccggc aaaccgccgc
     1261 cgtcgcacat cactactccc ataaagagtt ataaaaaaac ggccgcgttc tgaccagctg
     1321 tcaacttccc gattttgggc cgcccaacgt cactatcggc agcgagatcg ccgcgggatc
     1381 gcggcgtttt cgccgcggcg gcaacacggc ttcggcatac tcccagatct gctcgggtgt
     1441 ctcgaagatc ttctcgaacg accaggagtt gtcgtattcg caattgtcgg tcgtgtattc
     1501 gcgacagatc ttggggcgat tgagataaat gccgcagcga ttgtcggcag tcaggtactg
     1561 gcagcgtgtc atgaccagga gataccactg tcctttttcg acgtagatta ccgtctgtcc
     1621 gtgcgccaga taccagcgga tcgcgtcgta ctcatcccag gttgtgggat tggtgatcgg
     1681 cagagagaag taacgacaac atgccccggt gcaaaagtcg cagaggcatt cgccgggttt
     1741 gagctgctca cgcttgggtt tgcgcccttt agctctggtc gcggccatta aggctggtct
     1801 ccgtgcaggg gcggaccgtg ttcgagagcc tcgatgcagg tgtaagccgg aacatccctc
     1861 cgtcggtcaa gcacgtccgc cgcactgcgg taacgtcgcc ccggccatgc gttcggttcg
     1921 gttcgatcac gtgtttttcg tccggttgcg atagcgtgcg ctcacgcgga cgaggaaccg
     1981 attct
//
LOCUS       DATAQL010000551         8871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4024157, whole genome shotgun sequence.
ACCESSION   DATAQL010000551 DATAQL010000000
VERSION     DATAQL010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4024157"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VHS97_09535"
     CDS             <1..265
                     /locus_tag="VHS97_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448483.1"
                     /translation="AWLPWVAFLAMTAAGIIAAVDPGSGALSRTGAFSAQAQACALIA
                     LAAVLVPVLRRRSEKTDASVVPAADSEGSWINSTNGHAKDTEI"
     gene            complement(318..1229)
                     /locus_tag="VHS97_09540"
     CDS             complement(318..1229)
                     /locus_tag="VHS97_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3448484.1"
                     /translation="MRKSGIWLLLLALMAILQFAVGLETPFAAGASSRTQFPWKGHDA
                     TCSVRLPTPRYVSYGQFTTCPPKRVLVLGDSLALTMGIEMSIGQENWGTVMDGKAING
                     CGFVTGTDLEHNGAFVRENPACDNELATWTTDVHQFKPQAILIEMGWWDSFQHMINGN
                     VESLADPSYDNLVEQQIVHLTQGLRSATSANIYLLSVPWMNPSPLPNGQPEPAASTTS
                     HNEINSLLQAAAASSSATHFVDVSPYITPSGHFQTNVDGKVCRFSDGVHLYYAPNSSS
                     LHYVHTPCGQALQRGVLSMIRQGLSTK"
     gene            2040..3266
                     /locus_tag="VHS97_09545"
     CDS             2040..3266
                     /locus_tag="VHS97_09545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3448485.1"
                     /translation="MTLEETRRRVAPDRFLPSGDEIGTAPGDRKFRPDVEGLRAVAIG
                     LVVLFHAGVPGITGGFVGVDVFFVISGFVITGVLLRERAGSGRNSILAFYGRRARRII
                     PAATLVIVVTVLATYHWLGFILGGNAARDGRAAATFWVNFHFIATGTNYWTSQLPPSP
                     LQNFWSLSVEEQFYVVYPALFIAAAVIAVRRLHFHHRINMILGAGIVVSFLWSILQTS
                     SNATAAYFSPFTRAWELALGALVAANAGFLASVPQRVSAMLGWAGFGGILLTAFVYTS
                     ATPYPGYAAALPVVSTAAVIAGGTAGVTFGAETVLGLPPFQWLGKVSYSFYLWHWPLL
                     VIPMEIAGHPLSLKDNMGWVAVALTISVATYFLLENPIRHAKKLRQSWRLSIGLGAVL
                     VATSVAVTTFEIASHP"
     gene            3478..4428
                     /locus_tag="VHS97_09550"
     CDS             3478..4428
                     /locus_tag="VHS97_09550"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEX3448486.1"
                     /translation="MLVVIGGWMAPAFASGSRELTGPQMAAATQCQWPACAQVLSAVA
                     HGAKMQNLPANLTPSLQTAAGSVQPPQGLSKCVVGQQVVTTPSPCVYNATATTKRMVL
                     IGDSHADMWSQAVADLATANGYSLLFLAKIPCPLPLVSFWNGLNNTPNPQCTTWKNWA
                     IGRIQQFDPSIVIATTEDYHPTSSNLAPMSQSKYSNGLVTTLKDFAAPGRRVVLLGDI
                     PYLTKAGPICLSEHEGSIQSCSTPTDQAVSAQDQAAQRTAASKAGAEFINVIPWLCTT
                     KSCPAVVTNTDVYSDCCHITPNYAVYLEPVLSEALKLPAS"
     gene            complement(4463..6043)
                     /locus_tag="VHS97_09555"
     CDS             complement(4463..6043)
                     /locus_tag="VHS97_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3448487.1"
                     /translation="MTAVGSLALIVSLLAPSSAHAEVNDSNGQHSNVQCPAHDVSGGP
                     TGCYLVPPGIHKIKHVIIIMQENRSFDSYFGTYPGADGIPMNHGTATICVPDRATKTC
                     VAPFHDVYDVNGGGPHGEPDAIADVNEGKMNGFIKSVYGASNTCTQAADPACNHNLKN
                     NATVPDVMGYHAAAEIPNYWTYAKDFRLDDHMFEPVKSWSLPDHEYLVSGWSAKCKNA
                     RPSSCENNISGPYGLYQFDHAVDQELTQGTTSIDLAWTDLTYLLFKHRVSWGYYIQTG
                     QQPDCASDHMETCALPSQNYRTYGIWNPLPLFTDVQADQQEKNVQPLDNFLEQAHTGT
                     LPAVSWVTPAQIDSEHPPGGVHQGQAFVTSVINSVMEGPDWKSSAIFLSWDDWGGFYD
                     HVKPPTIDQNGYGLRVPAITISPYAKQGSIDHATLSSDAYLKFIEDDFLGGARLNPKT
                     DGRPDPRPDVRENAKQLGNLANDFNFNQAPRAPVLLPTNPPSDSPDLPAWFVGANQCY
                     GCTVPPTPVLTGDSPNPP"
     gene            complement(6504..8009)
                     /locus_tag="VHS97_09560"
     CDS             complement(6504..8009)
                     /locus_tag="VHS97_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3448488.1"
                     /translation="MANGQNPKVRCPSSDVAGGPTGCYLVPPGIHKIKHVIIVMQENR
                     SFDSYFGTYPGADGIPTKHGVPTVCVPDHSTKTCVAPFHDANDVNGGGPHDASDAVAD
                     VNGGKMNGFITSANKATKTCNQAADPACNHNLKGNTTKSDVMGYHTAAEISNYWTYAK
                     DFTLDDHMFEPVKSWSLPDHEYLVSGWAAKCKNKKPSSCKNAIDSYSLLQFDNAVEQE
                     LAQGTTSIDLAWTDLTYLLFKHQVSWGYYIQTGQQPDCANDHMETCKPAPQNYRTYGI
                     WNPLPLFTDVQNDNQVKNIQPLDNFLQQAHAGTLPSVSWVTPAQLDSEHPPASVHQGQ
                     AYVTSIVNAVMQSPEWKSSAIFLSWDDWGGFYDHVKPPKIDQNGYGLRVPAMTISPYA
                     KRGSIDHSTLSSDAYLKFIEDDFLGGARLNPKTDGRPDPRPDVRENAKRLGNLANDFN
                     FKQAPRPPVLLPTNPPSDSPDLPSWFVGANPCNGCTVPPTPRLTGDNPTPP"
     gene            complement(8370..>8871)
                     /locus_tag="VHS97_09565"
     CDS             complement(8370..>8871)
                     /locus_tag="VHS97_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448489.1"
                     /translation="AYTYVYGLVKDPYSQTVYGMKVARVPVGQSLDTSAWTYWNGTTW
                     IGGQLNANLINPGTILTGVMPQANGSGFVGVSIPGASFNGASVTLSYACSPTGPWSSP
                     QAVYTIPQLSEYPGEIAYTPTFHPEISGSGVVVSYNINNPTQGAQGQLLANVHEYQPQ
                     FLLLSG"
BASE COUNT         1629 a         2534 c         2790 g         1918 t
ORIGIN      
        1 tgcttggttg ccgtgggtcg cgttcctcgc catgaccgcc gcggggatca tcgcggccgt
       61 cgaccccggc tccggagcgc tctctcgtac cggcgccttc agcgctcagg ctcaggcgtg
      121 cgccctcatc gcgttggcgg cagtgctcgt tccagtcctc agacggcgct ctgagaagac
      181 tgatgcctcg gtggtgccag ccgccgattc cgaaggctct tggatcaact cgacgaacgg
      241 tcatgcgaag gacaccgaga tctgacacac gcgtcggcga ccagatctga cgtacgcgta
      301 agcctggacc gcacccgcta cttcgttgag agcccctgtc tgatcatcga aaggacgcct
      361 cgctggagag cctgtccgca gggagtgtgc acgtagtgca acgagctgct gttcggggcg
      421 taatagagat gaacgccgtc gctgaacctg cagaccttgc cgtcgacgtt tgtttggaag
      481 tgtccggaag gagtgatgta cggcgagacg tcgacgaaat gggttgcgct cgaagaggca
      541 gctgctgctt gaaggaggct attgatttcg ttatgactgg tcgttgacgc cgcaggctcc
      601 ggctggccat tcggtaacgg ggacggattc atccagggca ccgacagcaa ataaatgttg
      661 gcggaagtcg cggatctgag gccctgagtg agatgaacga tttgctgctc taccaggttg
      721 tcgtaggagg ggtcggccag ggattcgaca ttgccattga tcatatgttg gaaggaatcc
      781 caccagccca tttcgataag gattgcctga ggcttgaatt ggtgaacgtc agttgtccag
      841 gttgccaatt cgttgtcgca agccggattt tcacgaacaa atgcgccatt gtgttctaag
      901 tcggtcccgg tcacaaaacc gcatccgttt atcgccttac cgtccatcac cgttccccaa
      961 ttttcctggc cgatggacat ctcgataccc atggtcaacg ccaaggagtc accgaggaca
     1021 agtactcgtt tcgggggaca cgtggtgaac tggccgtaag acacgtagcg gggagtaggg
     1081 agtcgtacgc tgcacgtcgc atcgtggccc ttccacggga actgggttcg tgacgacgcg
     1141 ccggccgcga aaggagtttc taacccgact gcaaactgaa ggatcgccat cagggcgagc
     1201 agtagcaacc agataccaga cttacgcatc gaactccgtt tccccagcaa acagaaggcc
     1261 aagtcatttc atagctttat gatctgagaa tcggcttaga gcgaggcgta ttgtcgacca
     1321 ctttcgcttg tactgaactg aacagtctca acaacctgag agtgacctga atagagcggg
     1381 tcggactgaa agctgaatgt gacctgaata gagcgggtcg gattctgctt cccgtcaacc
     1441 tgagtgtgag ctgaatacag tgccgaacac atccgccgcg gcgcgtacag cttcaggttc
     1501 aacgctttgg acgcacttcg cgcacatcgc gacgcttcgg gcgaatggaa ttgtgcaatt
     1561 cggaatcgca aaggaaatag gagtcgtctc gcgcccaggg acgtggcgtc ggtcaaccac
     1621 cagagttcac tcgttgtctc gagagcaaga aacgatggac gttccagcca agttcgcgca
     1681 cgcgctctgg gtgctactcc gttttggatc cggtagcggg tccttcaacc atcagaggtc
     1741 actcgttgtc ttggaaccaa gacgtaggag gttccggcca ccttcgcgga ccctctcgaa
     1801 gagctactcc catttcggga aatgagagcc agcactgggg gacgacgtcg agcaaggaag
     1861 gggatcgcga gcgcgaactg cctgccgtgg tctcggattc ctcgcagaga gcggtatccc
     1921 ctggtgaagt gggtcggacc ttgtgagctg ttggtccggg ctgtcctcac aggacgccga
     1981 tcgagagtat tgctagggtc acggcgagcc tgcgcctttg gggaggggaa caagccgaaa
     2041 tgacgctgga ggaaacccgg cgcagggtcg cgcccgaccg attcctgcca tccggtgacg
     2101 aaataggcac tgcgcctggc gaccggaagt tccgtcccga cgtcgaggga cttcgcgccg
     2161 ttgctatcgg gcttgtcgtc ctttttcatg ccggagtgcc cgggatcacg ggcggctttg
     2221 ttggggtcga tgtctttttc gtgatctctg gtttcgtgat caccggggtg ctgctgcgcg
     2281 agcgcgccgg ctcaggcaga aattcgatct tggcgttcta cggccgacgc gcgaggcgca
     2341 tcattccggc tgcaactttg gtgattgtcg tcaccgtgct cgcgacctac cactggctcg
     2401 gtttcatcct ggggggtaat gcggcccggg atggccgagc tgcggccacc ttctgggtga
     2461 acttccactt catcgccacc ggaaccaatt attggacgtc tcagctgccg ccatcacctc
     2521 tccagaattt ctggtcgctt tcagtcgaag agcagttcta tgtcgtttat ccggcgctct
     2581 tcattgccgc tgcggtcatc gcagtcaggc gacttcactt tcaccatcgg atcaatatga
     2641 tcctcggcgc ggggatcgtt gtctctttct tgtggtcgat tctccaaacc tcgtcgaacg
     2701 cgacggcggc atacttttcg ccgttcaccc gggcgtggga actcgctttg ggagcactgg
     2761 tcgcggcgaa cgccggcttc cttgcgagcg tgcctcagcg ggtcagcgcc atgctcggct
     2821 gggcagggtt cggtgggata ctcctcacgg cctttgtcta tacgtctgcg acgccctatc
     2881 cagggtatgc cgccgcgctc ccggtggttt cgaccgctgc cgtgatcgcg ggaggtaccg
     2941 ctggagtcac gttcggtgcc gagacggtcc tgggcctccc gccgtttcag tggcttggca
     3001 aggtctcgta ctccttctac ctgtggcact ggccgcttct tgtcattcct atggagattg
     3061 caggccatcc actctcgctc aaggacaaca tgggctgggt agcggttgct ctcaccattt
     3121 cggtagcaac gtatttcctc ctcgagaacc cgatccgcca cgccaagaag ttgcgtcagt
     3181 cgtggcgatt gagcatcgga ctcggcgctg tcctcgtcgc tacgtccgtt gcggttacca
     3241 ccttcgagat cgcgagccac ccgtgaccga ggaagatcgc ttcggtcaca taactggtcc
     3301 cacggagggg taacgagagg attcgcgcgg atcaggagtg ggtacccagc tacagtcccc
     3361 gctccgagag tgcagtaggg gcatccattc cggatgaagg ggaggaaaat ggggtcacgt
     3421 tcacacgttc agcgaagcat cggtctcgca tccagagctt cgctcgttgt cggggccgtg
     3481 ctcgtcgtca ttggcggctg gatggcccca gccttcgcct ctggttccag agagctcact
     3541 gggccgcaga tggctgcggc aactcagtgt caatggccgg cctgcgcgca ggtgctgtcg
     3601 gccgttgcgc acggcgcgaa gatgcagaac ttgccggcca atctgacgcc gagcctgcag
     3661 acagcggcgg ggagcgtaca accaccccag ggcctttcga agtgcgtcgt tggccagcaa
     3721 gtcgtgacaa caccttcccc ttgcgtgtac aacgccacgg ccaccaccaa acggatggtt
     3781 ctcatcggag actcgcacgc cgacatgtgg tcgcaagctg tggcagatct ggccacggcg
     3841 aacggataca gcttgctttt tctcgccaag atcccttgcc cgttgccact cgtcagtttt
     3901 tggaacggtc ttaacaacac gcccaacccg cagtgcacca cctggaagaa ttgggcgatc
     3961 ggacggattc aacagttcga tcccagcatt gtcatcgcga ccacggagga ctatcacccc
     4021 acctcgagca atttggcccc gatgagtcag agtaagtact ccaacggcct tgtcacgacg
     4081 ttgaaggact ttgctgcacc tggtcgtcgc gtcgttctct tgggtgacat tccatacctg
     4141 acgaaagcgg gaccaatctg cctcagcgaa cacgaaggga gtatccagag ttgcagcacg
     4201 ccaaccgatc aggctgtatc tgctcaagat caggcggcgc agcgtacggc agcatccaag
     4261 gcgggcgccg agttcatcaa tgtgattccc tggttgtgca ccacaaaatc ttgccccgcg
     4321 gtggtgacga acaccgatgt ctattcagac tgctgccaca tcacccctaa ctatgccgtc
     4381 tatcttgagc cggttctctc ggaggcattg aaacttccgg cgagttgacc ctgcagagtg
     4441 cgcccgatcg gcgacgcgca gatcagggtg gattcgggct gtcgccggtc agaaccggcg
     4501 taggcggcac cgtgcatccg tagcactggt ttgccccgac gaaccaggcc gggagatcgg
     4561 gagagtccga aggtggattg gtggggagca gtaccggggc ccgcggcgct tgattgaagt
     4621 tgaaatcgtt ggcgaggttg cccagctgct tggcgttctc gcggacgtcc ggacgagggt
     4681 cggggcggcc gtccgtcttg ggattgagtc gggcgccgcc gaggaagtcg tcctcgatga
     4741 acttcagata ggcatctgag ctgagcgttg catggtcgat ggacccttgc ttggcgtacg
     4801 ggctgatcgt gatggctggc acgcgtaggc cgtagccatt ctgatcgatc gttggcggct
     4861 tcacgtggtc gtagaacccg ccccagtcgt cccaactcaa gaagatggca ctggacttcc
     4921 agtcaggacc ttccatcacc gagttgatca cggaggtaac aaaggcctgt ccctggtgga
     4981 cgccgccggg cgggtgctcg ctgtcgatct gggcgggcgt cacccaggag accgctggga
     5041 gcgttcccgt gtgcgcctgc tcgaggaagt tgtcgagcgg ctggacgttt ttttcctgtt
     5101 ggtcggcctg tacgtccgtg aacagcggca agggattcca gatgccatag gtgcggtagt
     5161 tctgtgaagg cagcgcgcac gtctccatat ggtcgcttgc gcagtcgggc tgctggccgg
     5221 tctggatgta gtagccccac gacacacggt gcttgaacaa caggtacgtg agatccgtcc
     5281 aggccaggtc gatcgaggtt gtaccttgcg tcaactcctg gtccactgcg tggtcgaatt
     5341 gatagagacc atagggaccc gagatattgt tctcgcagct cgatggcctc gcgttcttgc
     5401 acttggccga ccaaccggag accaggtatt catggtcagg cagtgaccat gacttcacgg
     5461 gctcgaacat gtggtcgtcg agcctgaaat cctttgcgta cgtccaatag ttggggattt
     5521 cggctgctgc gtggtaaccc atcacgtcgg gaaccgtcgc attgttcttc aggttgtgat
     5581 tgcacgcggg atccgctgct tgggtgcagg tgtttgaggc cccatagacc gatttgatga
     5641 atccgttcat cttgccttcg ttgacgtcgg cgatcgcgtc tggctctccg tgagggccac
     5701 cgccgttgac gtcatagaca tcgtgaaatg gtgcgacgca ggtcttcgtc gcgcgatcgg
     5761 ggacacaaat cgtggcagta ccgtgattca tgggtattcc gtccgcaccg gggtaggtgc
     5821 cgaagtacga gtcgaacgac cggttctctt gcatgatgat gatcacgtgc ttgatcttgt
     5881 ggatcccggg aggcacgaga taacagcccg tcggtcctcc ggacacgtcg tgtgctgggc
     5941 actggacgtt cgagtgctgg ccgttggagt cgttgacttc ggcgtgtgcg gaggagggtg
     6001 ccaataacga gacgatcagc gcgagactgc cgaccgccgt catgcgccgc aaaactcccc
     6061 ttccgaccag ctgggctagg tcggctgact ataggccagc aaaccatgta actggcatca
     6121 ttcagtggtt cccgacagcc cggtatgccc gtcctctgtg ccagcagctc ttgagtcaaa
     6181 ggggactgaa gaatgcttgg gaagagccca agaacgccct tgccagcaga caacggacgt
     6241 caagatcgcg ggttccaccg gttggccttc gttcagacgg ccccgaaccg cgactcacct
     6301 gagtagcacg actcgctgaa ctgagtctct tgcacacccc cgacgacgct gcgtatggag
     6361 tcgttgttgc tctacaaaaa ccgccagacg caatcagcgt tgagacgacg caagcggtac
     6421 cgctagtgcc tcctcaatgc gagtacgcgt gcgactgtga ggcgctcgcc cgagttctgg
     6481 ggcgagctcg caatcgcgca gcgttacgga ggagtcggat tgtcgcctgt caatctcggc
     6541 gttggcggca ctgtgcatcc gttgcaggga tttgccccga cgaaccagga cgggagatcg
     6601 ggagagtccg acggcggatt agtcggcagt agcacgggag gtcgcggtgc ctgcttgaag
     6661 ttgaaatcgt tggcgaggtt tccgagccgc ttcgcgttct cgcggacgtc cggacgaggg
     6721 tcggggcggc cgtcggtctt gggattgagt cgggcacccc cgaggaagtc gtcctcgatg
     6781 aatttcaggt acgcgtccga gctgagcgtc gaatggtcga tggaccctcg cttcgcgtac
     6841 gggctgatcg tcatggccgg aacccgtaag ccgtagccgt tctgatcgat cttcgggggc
     6901 ttgacatggt cgtaaaaacc gccccagtcg tcccaactca agaagatggc gctggacttc
     6961 cattccgggc tctgcatcac cgcattgacg atcgacgtca cgtacgcctg gccttgatgc
     7021 acgctggccg gagggtgctc gctgtccagc tgcgccggcg tcacccagga cactgacgga
     7081 agtgtccctg cgtgcgcttg ctggaggaag ttgtcgagcg gctggatgtt cttcacctgg
     7141 ttgtcgttct ggacgtcggt gaagaggggt agcggattcc agattccgta cgtgcggtag
     7201 ttctggggag ccggcttgca cgtctccatg tggtcgtttg cgcagtcagg ctgctggccg
     7261 gtctggatgt agtaccccca ggacacctgg tgcttgaaga ggaggtacgt caggtctgtc
     7321 cacgccaggt cgatcgaggt cgttccctgg gcgagctcct gctccacggc gttgtcgaat
     7381 tgaagcagcg aataggagtc gatcgcgttc ttacagcttg atggcttctt gttcttgcac
     7441 ttggccgccc agcccgaaac caggtattcg tggtcgggca gtgaccagga cttcaccggt
     7501 tcgaacatgt ggtcgtcgag cgtgaaatcc tttgcgtacg tccaatagtt cgagatttcg
     7561 gccgccgtgt ggtatcccat cacgtccgac tttgtcgtat tgcccttcag attgtgattg
     7621 cacgctgggt ccgctgcttg gttgcaggtc ttggtcgcct tgttggccga tgtgatgaaa
     7681 ccgttcatct tgccgccgtt gacatccgca accgcatccg acgcgtcgtg cgggccgcct
     7741 ccgttgacgt cgttggcatc gtgaaacggc gcgacacacg tcttggttga atggtccggg
     7801 acacagacgg tgggcacgcc gtgcttcgtg gggatcccat ccgctcccgg gtacgtgccg
     7861 aagtaggagt cgaacgagcg gttctcctgc atgacgatga tgacgtgctt gatcttgtgg
     7921 attcccggtg gcaccaggta gcagcccgtc ggtccgccgg cgacgtcgct cgaggggcac
     7981 cggaccttcg ggttttggcc attggccacc gcagcggcgg ttcgtgagct cagtccgggt
     8041 gttgacaaca agccggccac gagaaggagg cagacggcag cggacgcgag aagcttcaag
     8101 ggttcccccg gtgagaatcg aggtgcggag tcggccgact ataggtcggc ctgttccccg
     8161 ttttgtgtcg ccaacgggca ttggcagggg gttcccagcc gtgctctctt cccctcctcg
     8221 taggggtctg agctgctcga atctcgggcc gagtttcctc gatgggacca cgctgccttg
     8281 cccacgcggg tcgagcggct ttcaggtgct cgggatgctc aacggttcaa cgaaacggag
     8341 cccaacgcga gaagccggtt gatcggtgct cagccgctga gcaacaggaa ctgaggctgg
     8401 tattcgtgca cgttcgcgag caattggcct tgggcgcctt gggtggggtt gttgatgttg
     8461 tacgagacga ccacaccact cccggagatt tccggatgga acgtcggcgt gtatgcgatc
     8521 tcgcctggat actcgctcag ctgcggaatg gtgtagacgg cttgtgggct actccaagga
     8581 cccgtcggcg agcacgcgta ggacagggta acggaggcgc cgttgaagct cgcccctgga
     8641 atggacaccc cgacgaaacc ggaaccgttg gcttgtggca tgacgccggt aaggatcgtg
     8701 ccggggttga tgaggttggc gttcagctgt ccaccgatcc aggtggtgcc gttccagtag
     8761 gtccaggcgc ttgtgtcgag ggattggccc accggcactc gagcaacctt cattccgtag
     8821 actgtctgcg agtacggatc tttgaccaat ccgtacacgt aggtataggc g
//
LOCUS       DATAQL010000552         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2496705, whole genome shotgun sequence.
ACCESSION   DATAQL010000552 DATAQL010000000
VERSION     DATAQL010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496705"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..2067
                     /locus_tag="VHS97_09570"
     CDS             253..2067
                     /locus_tag="VHS97_09570"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX3448490.1"
                     /translation="MFLRRHRRTKNGKTHIYYALVESVRTEAGPRQRVVAHLGELNHE
                     QERRWQRTVRFHNQQGEDRQLRLFPDDNQVSSPRDPDIVRINLKSVGWANPRQFGDVW
                     LALWLWRHLKLDEILDRHVPQGKETIRPADIVAIEVINRLCSPCSEFELAEHWYASTG
                     LDDLLGIPDSAVTKDRLYRTLDQLLVGQMAIECDLEKQLGTLFRLDYSLVLYDLTSTY
                     FEGLAVENELAQRGYSRDHRSDCKQIVVALVVTKDGFPLAHRTFAGNTQDLKTVQTIV
                     GEIERQFGTSRRIWVMDRGMISDESLKFLGESNRRYLLATKRGELADFQADLGKTGWS
                     PLPKNPQVQVKLFERESVHYLLARSRPRRGKERAIRRRQRRGLATDLKKLSALVDAGK
                     LKKRDKIVERVGRLKERYPKARGFVQINVTATKQPRLEWTWDQAKYRQALATDGAYLL
                     RSNQPGWTAAEFWKTYIQLTIVERAFRVLKSELLLRPIWHHYSGRTRAHVFVCVLAYA
                     LWKTLDHLAKRAGLMTEIRKPDLRRPRSSPKARRMTPQMILRELRELKIGDILMSTSD
                     GQQLALRRVARPGPSQARILDALKLVLPERLATPDRIL"
BASE COUNT          498 a          701 c          695 g          456 t
ORIGIN      
        1 cctcgctggc gcttcgggct cgcatggtgg ccgtcagcgc gggacgcggg cgcaagccaa
       61 tcgtgaaccg gtctaaccta tgttgcgtag gaaagaatta gttttggccc agaatccgag
      121 cgccaaccgc gaaaaacccg cgaaaaacgt agtgaaaacg ctcgaaaacc gatgtagtga
      181 aaatctattg tagaaattct gtcttgattt gatggctgcc cccgctcatt atctgtgggc
      241 ggggaggccg ctatgttcct gcgacgccat cggcgaacca agaacggcaa gacgcacatc
      301 tattacgctc tggttgaaag cgtccgcacc gaggccggtc cacgacaacg cgttgttgcc
      361 cacctcggcg agctcaacca cgagcaagaa cgtcgctggc aacgcacggt ccggttccat
      421 aaccagcagg gcgaagatcg gcagcttcgg ctctttcccg acgacaacca ggtttcctcg
      481 cctcgtgacc ctgacatcgt tcggatcaac ctgaaatccg tggggtgggc caatccacgg
      541 caattcggcg acgtctggct ggcgctttgg ctctggagac atctgaagct cgacgagatt
      601 ctcgaccggc atgttcctca aggcaaagaa acgatccgcc ccgccgacat tgtggctatc
      661 gaggtaatca accgtttgtg ctcaccttgc tcggaatttg agctggcaga gcactggtat
      721 gcgtcgaccg gcctcgatga ccttctcggt attcccgata gcgcggtcac caaggaccgc
      781 ctctatcgaa ccctcgatca acttctcgtg ggtcaaatgg cgatcgagtg cgacctcgag
      841 aaacaattgg gcacgttgtt tcgactcgat tacagcctgg tgctctatga cctcacgagt
      901 acttacttcg aagggttggc cgttgaaaac gagctggcgc agcgaggata ctcccgcgat
      961 caccgtagcg actgcaaaca gattgtcgtg gccttggtcg tgaccaaaga tggcttccca
     1021 ctggcacacc gcacattcgc tggcaatacc caggacctga agaccgtaca aaccatcgtc
     1081 ggcgagatcg agaggcagtt cggcacgtct cggcgcatct gggtcatgga ccgcggcatg
     1141 atcagcgacg agtcgttgaa gtttcttggt gaatcgaacc gccgctacct gctggccacc
     1201 aagcgcggcg agctagccga tttccaagct gatttgggaa agactggctg gagcccactc
     1261 cccaagaatc cccaggtgca agtcaaactc ttcgagcggg agtcggtcca ttacctgttg
     1321 gcacgaagtc ggccgcgccg cggaaaggaa cgggcgatcc gccgccgcca gcgtcgcggc
     1381 ctggcgaccg atctcaagaa actcagcgct ctggtcgatg ccggaaagct caagaagcgg
     1441 gacaagatcg tcgagcgcgt cgggcgattg aaggagcgct atccgaaagc ccgcggcttc
     1501 gtccaaatca acgtgacagc gacgaaacag ccccggctgg aatggacgtg ggaccaagcg
     1561 aagtaccggc aagctttagc cacggatggg gcctacttgt tacgaagcaa ccaacctggc
     1621 tggacggccg ccgaattctg gaaaacgtat attcaactca cgatcgttga acgtgctttc
     1681 cgagtcctca agagtgaact tctcttgcgg ccgatctggc atcattacag cgggcgtacg
     1741 cgcgctcatg ttttcgtctg cgttctggcg tacgcgctct ggaagaccct ggaccatctc
     1801 gccaagcggg ccgggctgat gaccgagatc cgtaaacccg atctgcgtcg tccccgatcg
     1861 tctcccaagg ccagacggat gacgccgcag atgattctcc gtgagctgag ggagctcaag
     1921 atcggcgaca tcctgatgag cacgtcagac gggcagcaac tggccctgcg tcgggtcgct
     1981 cgacctggcc cgagccaagc gcggatcctg gatgccttga agctggtatt gccggagcgg
     2041 ctcgcgaccc cggatcggat tttgtagtga agacctggcg gggcttgcgg cctgcaccta
     2101 tggagattac cgaggatttt cggtgtttac atccatgcaa ctgcgcaact caggccagct
     2161 cgcggacgac atcgctcacg tcagtctatc cgcgggcggt ctcttcgtgc gcgaccttgc
     2221 tccgccagcg ggtacttcgc gccgtgtcag atgggcaagc ggagctggtg ttcagcgcca
     2281 gtctcggtcc cggccgcgag cgaggagagc gtcacgccga ccagcctgat gcccttgctg
     2341 acggggaaga
//
LOCUS       DATAQL010000553         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_359518, whole genome shotgun sequence.
ACCESSION   DATAQL010000553 DATAQL010000000
VERSION     DATAQL010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359518"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1595)
                     /gene="xylB"
                     /locus_tag="VHS97_09575"
     CDS             complement(60..1595)
                     /gene="xylB"
                     /locus_tag="VHS97_09575"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX3448491.1"
                     /translation="MSITLGIDIGTSATKTIAIDEKGTILASASAEYPCDHPRPGWSE
                     QSPGLWWDATKKTVQQVLSSGKFQASDVAGVGLSGQMHGSVFLDSAGDVIRPALLWND
                     QRTAAECQEIETKAGGRAALIRLVANPALTGFTAPKLLWVRKHEPASWERVRQVLLPK
                     DYVRYRLTGTFATEVSDASGTLMLDVANRRWSRELLAKLDIDPALLPSCHESQEVSGQ
                     VSELGSKATGLPVGTPVVGGGGDQPAGAVGNGIVRQGVVSATMGTSGVVFAHADTMGF
                     DPLGRLQRGCHAVPGAWHVMGVVLAAGGSFQWFRNELGKAEVEMARKKGEDPYFLLTD
                     EAAIAGPGAEGLFFLPYLTGERTPHFDPDAKGAWVGLTVRHGRAHLIRALLEGATFAM
                     RDSLELIREMGVKIDQVRLSGGGARNALWRQIQADIYGCDVHTLNSTEGPAFGVALLA
                     QVGTGGFASVLEACEATIRAVDSTHVEPRTKAYYDRAFPVYRQLYQDLRASFKNISLL
                     VDQ"
     gene            complement(1592..2437)
                     /locus_tag="VHS97_09580"
     CDS             complement(1592..2437)
                     /locus_tag="VHS97_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3448492.1"
                     /translation="MIEPVRRDAELLAEITRATPGPGSLFVWWLGQSGFLIKSRQGLL
                     AIDLYLSEHLTKKYESTNRPHIRMTRAPIRGHELQSVDLVLASHKHSDHLDPGTIPDL
                     LSASSGAILALPESIIDHAVQLGLPRDRLVGLDAGDIVDRTGFRIRAVPSAHEGLDTD
                     PNGRHLYLGFVIESEGRRLYHSGDSLAYPGLADRLGSDPFDVLFLPINGRDPARGVPG
                     NMTAAEAVDLATVVRPRFVVPHHYDMFTFNTVPALVFATEARRLPPGVEPRIFSCGER
                     WEIMP"
BASE COUNT          557 a          887 c          856 g          525 t
ORIGIN      
        1 agtgtcacga gaatctgccg aactttccgg taaaggcggc tcggagctgg tcggttgcgt
       61 cattgatcaa ccaataagct aatgttcttg aacgatgcgc gcaggtcttg ataaagttgg
      121 cgatagacgg ggaaggctcg atcgtagtac gccttggtcc gcggctcgac gtgcgtcgag
      181 tcgacggcgc gaatggtcgc ctcgcaggct tctaggaccg acgcaaagcc gcctgtgccg
      241 acctgagcaa ggagcgcgac tccaaacgcc gggccctctg tcgaattgag cgtgtggacg
      301 tcacaaccgt agatgtcagc ctggatctgc ctccagagag cgttgcgcgc acccccgccc
      361 gagagccgga cctggtcgat cttaaccccc atctcgcgga tcaactcgag gctgtcgcgc
      421 atggcgaagg tcgctccttc caggagagct cgaatcaagt gggcacggcc gtgacggacg
      481 gtcaagccaa cccaggcccc cttggcgtcg gggtcaaaat ggggcgtacg ttcgccggtc
      541 aggtagggga ggaaaaagag cccctccgcc cctggaccag cgatcgccgc ctcgtcggtg
      601 agcaggaagt agggatcttc acctttcttg cgtgccatct caacttcggc cttgcccagc
      661 tcgttgcgga accattgaaa gctaccgccg gccgcaagca cgacccccat gacgtgccac
      721 gcgcccggca cggcgtggca accgcgctgc agccttccca atggatcgaa gcccatggtg
      781 tcggcatgag cgaaaactac ccccgaagtc cccatggtcg ccgacacgac cccctggcgc
      841 acgattccgt ttcccaccgc ccccgccggc tggtcgccac cccctccaac gaccggcgtg
      901 ccgaccggca gccccgtagc tttggaaccg agctcactca cctggccgga gacctcctgg
      961 ctctcatggc acgagggcag aagcgctgga tcgatgtcga gcttcgccag gagctcgcgg
     1021 ctccagcggc gattggcgac gtcgagcatg agcgtgcccg aggcgtcgct cacttcggtc
     1081 gcgaaggtgc cagtcaaacg gtagcggaca tagtccttgg gcaaaagcac ctggcggacc
     1141 cgttcccagc tcgctggctc gtgcttgcgg acccaaagga gcttgggggc cgtgaatccg
     1201 gtcaaggcgg ggttggcgac caggcgaatc aaggccgcgc ggccaccggc cttcgtctcg
     1261 atttcctggc actccgccgc cgtgcgctga tcgttccaga ggagagccgg gcggatcaca
     1321 tcgccggccg aatcgagaaa gacagaaccg tgcatttgac cgctcaggcc gacacctgcg
     1381 acatcgctcg cctggaactt gcctgagctt agaacctgct ggacggtttt cttggtggcg
     1441 tcccaccaga ggcctggaga ctgttccgac cagcctggcc ggggatggtc gcacggatac
     1501 tcggccgacg ccgaggccag gatcgtccct ttctcgtcga tggcgatcgt cttggttgcg
     1561 gacgttccaa tatcgattcc cagcgtgata ctcacggcat gatctcccag cgctctccgc
     1621 acgaaaagat gcgcggttcg acccccgggg gaagccggcg cgcttcggtt gcaaacacaa
     1681 gggcgggtac cgtgttgaag gtgaacatgt cgtaatgatg cggcacgaca aatcgcggcc
     1741 ggacgacagt cgccaggtcg actgcctcgg ccgcagtcat gttccccgga acaccgcgcg
     1801 ccgggtcacg accgttgatc ggtagaaaga gaacgtcaaa cgggtcgctc cccagtcgat
     1861 cggccagccc aggatacgcc agactgtcgc cactatgata cagcctccgc ccctcggact
     1921 cgatcacgaa ccccaggtaa agatgacgtc cgttcgggtc ggtgtcgagt ccctcgtgag
     1981 ccgagggcac ggcgcggatc cgaaagcccg tgcgatcgac gatgtctccc gcatcgagac
     2041 ccaccagccg gtcgcgcggc aggccaagtt gtacggcgtg gtcaatgatc gattcgggca
     2101 acgcgaggat cgcgccggac gaggctgata gcaagtctgg aatcgtccca gggtcgagat
     2161 gatcggaatg cttatggctt gccagcacga gatcgacgct ttggagttca tgacctcgga
     2221 ttggagcgcg ggtcatgcgg atgtgcgggc gattcgtcga ctcgtatttc ttggttagat
     2281 gctcggagag atacaggtcg atggcgagca agccttgtcg cgacttgatc agaaagccgc
     2341 tctgaccgag ccaccataca aacagtgacc cgggaccagg agtcgccctg gtgatttccg
     2401 ctagcagttc ggcatcgcga cggaccggct cgatcattgc agttgttccc acccaaactt
     2461 gttgcacaaa tgatcgagat gaattgcgcc gatacccgcg tcccggtcgt caaagttcac
     2521 gaccttccca acatctagta tcacgcgcgg cgacgttaaa atgttagcct tggtccgaaa
     2581 ccgacggcca aatcctaagt gggcccgccg aagtcgtcgc gcagcgtagg tcgggctttc
     2641 tagcccgacg ttctactggg ggggcacaaa gagtcaggct ggaaagcctg acctacacaa
     2701 actgttctgc ggtctcactt agtcaaccag acttttcgtc caaccaattt tccttcctcc
     2761 tttggaactt tgtgtgaggt tccattccga ttcacggtaa atctcgcgtc cggtcaacag
     2821 tccgg
//
LOCUS       DATAQL010000554         6066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4940172, whole genome shotgun sequence.
ACCESSION   DATAQL010000554 DATAQL010000000
VERSION     DATAQL010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4940172"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VHS97_09585"
     CDS             complement(<1..276)
                     /locus_tag="VHS97_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448493.1"
                     /translation="MTTQRALGSVPDQNSRLAQHSASRTPWVCRAPLLVAAGLICLIS
                     AFNLARLVSAPAPRDPWEATEVIEAWRSLRGMPVYELKPDGHSTHVYG"
     gene            complement(313..1053)
                     /locus_tag="VHS97_09590"
     CDS             complement(313..1053)
                     /locus_tag="VHS97_09590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3448494.1"
                     /translation="MSYVYVGTELDLFASAEHWKLYVRRQVQAFLGRQVLEVGAGHGG
                     TTRVLCDDRPERWLCLEPDSSLADRLISSISGGELPDCCEARNGTLADLDEGDRFDTI
                     LYMDVLEHIDDDRAELARAAEKLQEGGCLIVLAPAHLWLFTAFDEGIGHFRRYTKHTL
                     KAVAPDGLALARLAYLDSIGMLASLGNKFVLKSAMPSPGQIAVWDRLMVPLSRLVDPF
                     FGYSLGKSVLAVWRKPNRNDRRRADSRD"
     gene            complement(1057..2226)
                     /locus_tag="VHS97_09595"
     CDS             complement(1057..2226)
                     /locus_tag="VHS97_09595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3448495.1"
                     /translation="MERHPHRSRIDSRPHVLINKRSIASKRASDWQSGGNDDSVDDGL
                     PENKTGQIVILIPIFDDWAALAQLLPKLDSVMASHFRTVDVLVVDDGSTTLPDGTLES
                     GAFAALRRVDVLRLRRNLGHQRAIAVGLAYMEDCLSAGAVVVMDGDGEDDPADVPRLL
                     ERLEETGDGTIVFAERARRSESLVFRVFYVLFKLLHRFLTGQGVRVGNFSAIPRRRLS
                     SLVVVAEMWNHYAAAVFRSRQPYCMIPTQRSQRLCGNSNMNFVSLVTHGLSAISVYSD
                     VVGVRLLVMAIVLACLTLGGMIAAVIVRLTTNMAIPGWASYIVGIMLILFVQAVMAAF
                     VFSFAILGSRHGTTFLPRRDYSIFIESIWNCDGSGKSASPLPEPEPKSQVLASRS"
     gene            complement(2239..4065)
                     /locus_tag="VHS97_09600"
     CDS             complement(2239..4065)
                     /locus_tag="VHS97_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448496.1"
                     /translation="MATASPSLRHALRQPSVSANPSTLRQSEKRSAWSRLGRQLCLVV
                     LIAGAVVLPRSYLIAQAHSESNDDAYHLRRGLIFLTRSLAGSNLELNDPPLGEGIVAI
                     PMFVTNLIEGRELADDKLYDVPQRAETIAVRTALWNSLLFVGFLGVVFTWCRAVYGMW
                     PAWLAVALFLIEPNFAAHVPIPALDVLGVEGIVIGCFLAWRYFERPTTARLIAMGFGL
                     AFALLLKHTALMLPPVILALAGLHWVVRPWMERQDWAIWKGTIRDRLRSSALLGVIVP
                     VAIWAFTLFDCSPPLNRSAVERQTKGSNGSPVSRGKALRVALERKLHLDAPWPAGCYL
                     LACRLGIGHAMSGHRSYLNGDRYDKGRWSYFPVVASYKVPIGIGVVFLVSFFTIWWTP
                     PRWAEWGLFVPILAWTLFALGSKVNLGFRHFLPAYAFMLMFASRCGVGPSRRWPVLAW
                     AAVAAAGIHALAYHPDYLCFINSPRSKPYLAISDCNVDWGQALKEVRAWLDDHPQNEK
                     KVSLYYFGNEDGAIPYYLNERVVALDQYSPRPTGGLLMISVVRLAGAYEDHDPYAALR
                     SHDPDDVIGNSIFVFDLDRLGGGSPFRWPPPKSWPIRLSKTP"
     gene            complement(4208..>6066)
                     /locus_tag="VHS97_09605"
     CDS             complement(4208..>6066)
                     /locus_tag="VHS97_09605"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX3448497.1"
                     /translation="DSKLAEPKQGEPVEEAKPSGRESSVGSAITEAQKRQEGTSRRLA
                     RLVILSDFANNAGSDPLESARRLKGQGVPVVTVGLGTENAGAVHRDIMLRDIIAGPTV
                     FVKNKADVKVSMRAPGFANQTLDVELWVEGQAVPVAKTRVKVPEGSDVIPITGLKYTP
                     LTPGEKQITVKVAKQDGELVVTNNEISTFLTVLSGGLNVLFLQGANFTWDYRFLMRAI
                     ERSPDIQVEGVVIRRKAEGETGEIADAEFAQGRYNVYVLSDLPADFLTPKQHRLLVDT
                     VKKGAGFMMLGGRSSFGAGGWAGTPLEEILPAAIHPGDGELEPPGGIKVEPTTEGLDS
                     FVLQVGANRQETKRIWDALPPILGSNRFGERKALAKVLATSPAPDAEPLMLSLEVGNS
                     RTIAYGGDTWVWARASEEGRLAHRKLWRQLVFWLSHKENDSENRVKLTLERRRVAAGE
                     KLEISATARDSKGVSIPNVKYETKVEREGPDPASEPVDLYDQVEEARGSIFAIEKLGQ
                     PGNYSVTTIARRDGQEIGRDKGRFLVYQDDRELENPSADLKLAREIAELTGGEPVNPE
                     RLGSYLKGIDRSVYTEYLSPSEYKVWDNWPFLLIFAALLTLEWWVRKRHGWV"
BASE COUNT         1266 a         1853 c         1788 g         1159 t
ORIGIN      
        1 accatagaca tgcgtcgaat gtccgtccgg cttgagttca tagactggca ttccccgcaa
       61 actgcgccag gcctcgatga cttccgtcgc ttcccagggg tctctgggcg ctggcgcgct
      121 caccagacga gcgagattga acgcactgat caaacagatg agcccagccg ctactagcaa
      181 tggcgcacgg cagacccacg gcgtgcgaga cgcggaatgc tgggccaatc gtgagttttg
      241 atcaggtacg gaaccaaggg cccgctgcgt cgtcatggca agtcatcctt gaaaaaagcg
      301 gcgtctggca gttcagtccc ggctgtctgc acgccgtctg tcatttcgat ttggcttcct
      361 ccacacggcc aggaccgact tgccgagaga gtagccgaaa aacgggtcga ccagtcgcga
      421 tagcggaacc atcaatcgat cccacacggc gatctgaccg gggctgggca tcgcgctttt
      481 gagtacgaac ttattgccca gcgatgcgag catgccgatt gagtcgagat atgccagtcg
      541 ggcgagagca agtccgtcgg gagcaacggc cttgagggtg tgcttggtat atcgccggaa
      601 gtgcccgatt ccctcgtcga aagcggtgaa tagccaaagg tgggcaggcg cgagtacgat
      661 caggcagcca ccctcttgca gtttttcggc agccctggcg agctcggcgc ggtcgtcgtc
      721 aatgtgctcg agtacgtcca tgtagaggat cgtgtcgaat cggtccccct cgtccaggtc
      781 ggccaaggta ccatttcgcg cttcacaaca atccggtaat tcgccaccag agatcgatga
      841 aatcaggcgg tcagcgagtg acgagtcggg ctccaggcag agccagcgct cgggcctgtc
      901 gtcgcagagg acacgcgtcg tgccgccgtg tcccgctcca acctctagca cctgccggcc
      961 caaaaatgcc tgcacctgac gtcgaacgta taatttccag tgctcggcgc tcgcgaaaag
     1021 gtcgagctct gttcccacgt agacgtatga caagtattaa ctcctagacg caagcacctg
     1081 actcttcggt tctggctctg gcagcggcga tgcggatttt ccggatccgt cacaattcca
     1141 gatggactcg atgaagatgg aataatcacg tcggggcaag aacgtcgtgc cgtgtcgcga
     1201 gcccaggatc gcaaagctga aaacaaatgc ggccatgacg gcttgaacaa acaggatgag
     1261 catgatgccg acgatgtaac tcgcccaacc tggaatggcc atattcgtcg tcagacgcac
     1321 gatcactgca gcgatcattc ctccgagcgt caaacacgcg agcacgattg ccattaccag
     1381 aaggcggacg ccgacgacat cgctgtagac cgagatcgcg ctcaaaccgt gtgtgacgag
     1441 cgaaacgaaa ttcatgttcg agttgccgca aagccgttgg gaccgttggg tcggaatcat
     1501 gcagtatggc tgccgcgatc ggaatacggc cgctgcgtag tgattccaca tctcggccac
     1561 cacgaccagg ctcgagagcc gccggcgcgg gatggcactg aagttgccca ccctcacgcc
     1621 ctggccggtc aggaaacggt gcagcagctt gaacaacacg tagaacactc ggaaaacgag
     1681 cgactcagat cgccgcgccc gttcggcgaa cacgatcgtc ccgtcccctg tttcctcgag
     1741 tcgctcgagc agccgcggaa cgtccgctgg gtcatcctcg ccatcgccat ccatgaccac
     1801 gaccgcgcca gctgaaagac agtcctccat gtaagcaaga ccaacggcga tcgctcgctg
     1861 atggccgagg tttcgccgaa ggcgcaacac atcaactcgg cgcaaagccg cgaacgctcc
     1921 gctctccagc gttccgtcgg gtagagtggt cgagccgtcg tcgacgacga gaacgtcgac
     1981 cgtgcggaag tgcgacgcca tgaccgaatc cagcttcgga agcaattggg ccaatgcggc
     2041 ccaatcgtcg aaaatcggga tcaggatgac aatctggccg gtcttgtttt ctggcaatcc
     2101 gtcgtcgact gaatcatcgt tgccgccgct ttgccaatcc gacgcgcgtt tgctcgcgat
     2161 tgatcgtttg ttgatcaaaa catgcgggcg agaatcgatc cggctacgat gtgggtgacg
     2221 ttccatgtct gctagccttc aaggagtttt tgacaaacga ataggccaac ttttcggagg
     2281 gggccagcga aatggagagc cgcccccgag ccggtcaagg tcgaagacga agatgctgtt
     2341 gccgatgacg tcgtcggggt catgggaccg gagcgccgcg tatgggtcat gatcctcgta
     2401 ggcaccggcc agtctcacga cgctgatcat gagtaagccg ccagtcggcc gcggcgagta
     2461 ttggtcgagc gccacgaccc tttcgttcaa gtagtacggt atcgcgccat cttcattccc
     2521 aaagtaatag agagagactt ttttctcgtt ctgggggtga tcgtcgagcc aggccctgac
     2581 ctctttcagt gcctggcccc aatcgacgtt gcaatcgctg atcgccaggt acggcttgct
     2641 ccgtggcgag ttgataaagc aaagataatc ggggtgataa gcgagcgcat ggattccggc
     2701 cgcggctacg gcagcccacg cgagcacggg ccaccggcgg cttggcccga cgccgcagcg
     2761 gctagcaaac atcagcatga aggcatacgc tggcagaaaa tggcggaaac cgaggttgac
     2821 cttggagccc agtgcgaaca aggtccaggc caagatcggc acgaacaaac cccactcggc
     2881 ccaacgcggc ggcgtccacc agatcgtgaa gaacgagacg aggaagacga cgcctatgcc
     2941 gattggaacc ttgtacgagg cgacaaccgg aaaatagctc catcgtcctt tgtcgtaccg
     3001 atcgccgttt aagtacgacc ggtgtcccga catggcgtgt ccgatcccca gccggcaggc
     3061 gaggaggtag cagccggccg gccatggcgc gtcgaggtgg agttttcgct ccagagcgac
     3121 acgaagcgcc ttaccccgac tgacagggct cccattgctc cccttggttt gccgctcgac
     3181 tgccgagcgg ttcaacggcg ggctgcaatc gaatagcgtg aaagcccaga tggcgactgg
     3241 aacgatcacc cccaagagcg ccgacgagcg caggcggtcg cggatagtgc ccttccagat
     3301 tgcccagtcc tgacgctcca tccacggccg gactacccag tgcaagcccg ccagcgccag
     3361 gatgaccggg ggcagcatca atgcggtatg cttcagcaac agcgcgaatg ccaacccaaa
     3421 acccatggcg atcaatcgcg cggtcgtcgg ccgctcgaag tagcgccagg ccagaaagca
     3481 gccgatcacg atcccctcga cccccagaac gtcgagcgcc gggatcggta catgggcggc
     3541 aaagtttggc tcgatcagga atagagcaac cgccaaccac gccggccaca tcccatagac
     3601 ggctcggcac caggtaaaga cgacgccaag aaagcccacg aacaggagtg aattccagag
     3661 ggccgtacgg accgcgatcg tttccgcccg ttgggggacg tcgtacagct tgtcatccgc
     3721 cagttcgcga ccttcgatga gattcgtcac gaacatcggg attgcgacga ttccctcgcc
     3781 cagcggcgga tcgttaagct cgaggttaga cccggcgaga cttcgagtga ggaatatgag
     3841 cccccgtcgc aggtggtaag cgtcgtcgtt cgactcgctg tgcgcctggg caattagata
     3901 ggaccgcggt agcactacgg cgccggctat caatacgacc aagcacaact gccgccccaa
     3961 tcgggaccag gcggagcgtt tctcggactg cctcaaagtg gaaggattgg ccgagaccga
     4021 aggctggcgc agcgcatggc ggagtgaggg tgaagcggtc gccatccata gcggtcctga
     4081 attcgcggtg tctctggcta gttgctcgct tgagtcatcc atcactcaag gctgttgctg
     4141 gacaagacgc gatgtcgatg gaaagggaac ttagcctatc gccccggtat tggcaagatg
     4201 tctcagctca gacccacccg tgtcgtttgc ggacccacca ttccagcgta aggagtgccg
     4261 cgaagattaa caagaatggc cagttatccc agaccttgta ctccgatggg ctcaagtatt
     4321 cggtgtagac ggagcgatcg attcccttga gatagcttcc taatcgctcg ggattgactg
     4381 gctcgccgcc cgtgagctcg gcgatctcgc gcgccagttt gaggtcggcg gatgggtttt
     4441 ctagctcgcg gtcgtcctgg tagacgagga accggccttt gtctctgcca atttcttggc
     4501 cgtcgcggcg ggcgatggtg gtgaccgaat agttgccggg ctgtcccagc ttttcgatcg
     4561 caaaaatcga accgcgtgcc tcctccacct ggtcataaag atcgacaggt tcggatgcgg
     4621 gatccgggcc ctcgcgctcg accttggttt cgtacttgac gttagggatg ctgacaccct
     4681 tcgagtcacg ggcggtggca gaaatctcga gtttttcgcc ggcggccact cgccggcgct
     4741 cgagggtcag cttgacgcgg ttttcgctgt cgttttcctt atgagacagc caaaaaacga
     4801 gctgtctcca caacttgcga tgagcgagcc gaccctcctc cgaagcgcgg gcccaaaccc
     4861 aggtgtcacc gccataagcg atcgtgcggc tgttaccgac ttcgagggag agcatcagcg
     4921 gctccgcgtc aggtgcgggt gacgtcgcca gcactttggc caatgccttc cgctcaccaa
     4981 agcggttcga acccaggatg ggcggaagcg catcccagat tcgcttggtc tcctgccggt
     5041 tggctcctac ctgcaacacg aaactgtcga gaccctccgt cgtgggctcg actttgatac
     5101 ccccgggcgg ctcgagctca ccgtcacccg ggtggatcgc cgcgggcaga atctcttcta
     5161 gcggagtacc cgcccagccg ccagcaccaa aactggatcg gccgccgagc atcatgaaac
     5221 ccgctccctt cttcacagta tcgaccagga gcctgtgctg ctttggagtc agaaaatctg
     5281 ccggcagatc ggaaaggacg taaacgttgt agcgaccctg agcgaactca gcatcggcga
     5341 tttcgccggt ttctccttca gccttacgac gaatcaccac accctcgacc tgaatatccg
     5401 gcgacctctc gatggctctc atcaagaatc gatagtccca cgtgaagttg gcgccctgga
     5461 gaaacaagac gttcagcccg ccgctcaaaa cggtcaagaa ggtactgatc tcgttgttgg
     5521 tgactaccaa ctcgccgtct tgtttggcca ccttgactgt gatctgtttt tcccccggcg
     5581 tcaggggcgt atatttcagg ccggtgatcg gaatcacatc gctcccttcc ggcaccttga
     5641 cccgggtttt tgcgaccggc actgcctggc cctcgaccca cagctcgacg tcgagcgttt
     5701 ggttggcaaa acccggggcc cgcatggaaa ctttgacgtc ggccttgttc tttacgaaga
     5761 ccgtcggtcc ggcgatgatg tccctcagca tgatatcgcg gtgaacggca cccgcgtttt
     5821 ccgttcccag tccgacggtg accacgggca caccctggcc cttgagtcgg cgggccgact
     5881 cgagaggatc cgagcccgca ttgttggcga aatccgagag aatcaccagc cgcgccagcc
     5941 gacgggacgt tccctcttgc cgcttttggg cttccgtgat cgccgagccc accgaggact
     6001 cgcggccact gggtttggcc tcctccaccg gttcgccttg cttgggctcg gccagcttgg
     6061 agtcga
//
LOCUS       DATAQL010000555         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2803271, whole genome shotgun sequence.
ACCESSION   DATAQL010000555 DATAQL010000000
VERSION     DATAQL010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2803271"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VHS97_09610"
     CDS             complement(<1..494)
                     /locus_tag="VHS97_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3448498.1"
                     /translation="MRRRGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRIQCTNN
                     LKQLALAVMNYADVNGALPPTAICSSSNTSNSCFGQTPDFSMKARLLPFFEQTSIYNA
                     LNFSAVTFSPLNITSRFSKISTFLCPSDASQASVSVTTGTFTGTLPLTNYPNNIGTFA
                     PEATT"
     gene            complement(598..1506)
                     /locus_tag="VHS97_09615"
     CDS             complement(598..1506)
                     /locus_tag="VHS97_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3448499.1"
                     /translation="MSALVVFVSLAAAEDVKRPRITGVAHIAVYAHDPEETIAFYRDF
                     LGFGEPYRLKRGGGELHLAFIKINDRQFVEVFPEKAAGTDRLNHIALEVEDAEAMRAY
                     LASRGVKVPNKVPVGKIGNANFNIIDPDGHTVEIVQYLPDGWSMRQKGNNMPDSRIGS
                     AIKHVGIAVGSLDKAMAFYRDILGFGETWRGSSSGKALNWVNVRVPDGDDYVEFMLYK
                     TAPALDRLGTMHHLCLEVSDVEQAKSTLQGRATGRHYTRALEIKTGVNRRRQLNLYDP
                     DGTRIELMEPKTVDGSPAPSSTAPPP"
     gene            complement(1609..>3177)
                     /locus_tag="VHS97_09620"
     CDS             complement(1609..>3177)
                     /locus_tag="VHS97_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX3448500.1"
                     /translation="DPSDLRAFDPSSTGGDARRAYSLRIDSLGRNGSSSRRSTRPWIL
                     VAIAAAACVLALLINGWFFWSRPLRVSQGPGAPTIAKPDQPRPAGELQKIADANPVAI
                     MIKLDGAVWEPAGGPGPAERDVLAARRLRLRSGRATLAFLSGVTLTLEGPGDLELISI
                     DRVFCREGRLRARVPKGAEGFVVGSPGSAVVDMGTEFAINVEADGRSRVMVFEGAAEA
                     ALLGTAGSPERTQLVERSKAFDLDPRRRRITESGAQTTGFVTATPLSVAPLVLGPAYA
                     GAVLRSLPRGYWRFESISGGAIPNEVQGGAALRVNGPVKIAGGPQGNGHAVFKANEPD
                     QFLYSNEIWETTRQPGHAIELWFAAEGISHASLVGLFPAVERRNNAGNRQYVHTFFVE
                     LTARDRQSLYRPASVRFLHRWPLDFTIGDNLMSQHLYIPQRWHHVVAQKNGNRMELYF
                     DGEPDHSMTLDIDHPTVSCRLVVGRRTPDLAYSHDLRTFVGRLDELAVYNHPLSADEI
                     RQHFRLANLEDLPP"
BASE COUNT          588 a          974 c         1037 g          578 t
ORIGIN      
        1 gtggtcgcct ccggggcaaa tgtgccgatg ttgttcgggt agttggtcag aggcagcgtt
       61 cccgtgaacg tgccggtcgt caccgagact gacgcctggc tcgcgtccga cgggcaaaga
      121 aaagtcgaaa tcttgctgaa acggctcgtg atgttcaacg ggctgaacgt cacggccgag
      181 aagttcagtg cattgtagat cgacgtctgc tcgaagaagg ggagcaagcg ggccttcatc
      241 gagaagtcgg gggtctggcc aaagcaggag ttgctcgtgt tggagctgga gcagattgcc
      301 gtcggcggca gggccccatt gacgtcggca tagttcatga ccgccagtgc aagctgcttc
      361 aagttgttgg tgcactggat ccgtcgagcc gcctcgcggg ccgcctgcac cgcgggcaga
      421 agcagggcga tcaaaacggc gatgatggcg attacaacca gcaattcaat caacgtgaat
      481 ccacgtcgtc gcatgaaaat actccttctc gctgagaagg ccggagagag cgccacttcg
      541 gagtgggctg ccgcgcggcg gcccgaggag agtcatccga ggtagcttcg tgcacgatca
      601 aggcggcggg gccgtggacg acggcgcggg actgccgtcc acagtcttgg gctccatcag
      661 ctcaatgcgc gtgccgtcag ggtcgtacag gttcagttgt cgccggcggt tcacaccggt
      721 ttttatctca agtgcgcggg tgtaatgccg gcccgtcgcc cgcccctgga gcgtcgactt
      781 ggcctgctcg acgtcggaga cttccaggca gaggtgatgc atcgttccca gtcggtcgag
      841 cgccggggct gtcttgtaga gcatgaactc gacgtagtcg tcgccatcgg gcaccctcac
      901 gttcacccag ttgagcgcct taccgctcga gctgccccgc catgtttccc cgaatccgag
      961 gatgtcgcga tagaaggcca tcgccttatc cagcgatccg acggcgattc ccacgtgctt
     1021 gattgccgat ccaattcgcg agtcgggcat gttattcccc ttctggcgca ttgaccagcc
     1081 gtccggcaga tactgaacga tctcaacggt gtgtccatcg gggtcgatga tgttgaagtt
     1141 ggcgttgccg atcttgccga cgggaacctt gtttgggact ttgaccccgc gcgacgccag
     1201 gtaggcgcgc attgcttccg cgtcttccac ctcgagcgcg atgtggttca atcggtcggt
     1261 gccggctgcc ttctccggga acacctcgac aaactgccga tcgttgatct tgatgaaggc
     1321 gaggtgcagc tcgccgccgc ccctcttgag cctgtacggc tcgccgaagc ccaggaaatc
     1381 gcggtagaag gcgatcgtct cttcggggtc gtgcgcatag acggcgatgt gcgccacgcc
     1441 ggtgatccgc gggcgcttca cgtcctccgc ggccgcgagc gacacgaata ccaccagagc
     1501 cgacattgtc gttatcagtc ttctgatcac gaatgagccc cctctcgatt caaaggtcgg
     1561 gtatgtgacg cgaggcgaga ccggctcgcg gcgaatgttt ggtcgtattc atggcggcag
     1621 gtcctccagg ttcgccaacc ggaaatgctg ccgtatttcg tcggccgaca gcggatggtt
     1681 gtagaccgcg agctcgtcca gacggccgac gaacgtgcgg aggtcgtggg aataagccag
     1741 gtcaggcgtc cgacggccca cgaccaggcg gcacgacaca gtcgggtggt cgatatccag
     1801 tgtcattgag tggtcgggct cgccgtcaaa gtaaagctcc attcggttgc cgtttttctg
     1861 ggccacgacg tgatgccatc tctgaggtat ataaagatgt tgcgacatga gattatcgcc
     1921 aatagtgaag tcgagcggcc accggtgcag gaaccgcact gacgcgggcc ggtacaacga
     1981 ctggcggtcg cgggcagtga gctccacgaa aaacgtgtgc acgtactggc ggttgccagc
     2041 gttgttgcgt cgctcgacgg ccgggaacaa gccgacgagc gaggcgtggc tgatgccctc
     2101 ggcggcaaac cagagctcga tcgcgtgacc gggctggcgg gtcgtctccc agatctcatt
     2161 gctatacaag aactggtctg gctcgttggc cttgaacacg gcatgtccgt taccctgggg
     2221 accgccggcg atcttgaccg ggccgttgac ccgcagcgcc gccccgccct ggacctcgtt
     2281 ggggatcgcg ccgccagaaa tggactcgaa ccgccagtaa ccccgcggaa gcgatcgcag
     2341 gacggcgccg gcgtaggccg ggcccagaac caagggcgcg acggacaaag gagtggcggt
     2401 gacaaaccca gtcgtctgcg cgccggattc tgtaatgcgg cgccggcggg gatcaaggtc
     2461 gaacgccttg ctgcgttcca cgagctgggt acgctccggt gagccggcgg tgccgagtag
     2521 ggccgcctcg gcggcgccct cgaagaccat gactcgcgac cgcccgtcgg cttcgacgtt
     2581 gatcgcgaat tcggtcccca tgtcgactac ggccgacccg ggggagccga cgacaaaacc
     2641 ttcggctccc ttgggaactc gcgcccgcag ccggccttcc cgacagaaca cccggtcgat
     2701 cgagatgagc tcgaggtcgc ctgggccctc caaagtcagc gtcacgccgc tcaagaaggc
     2761 aagcgtggca cggcccgaac gcaggcgcag ccgacgtgcc gccagcacgt cgcgttccgc
     2821 ggggccgggg ccgccggccg gctcccagac ggcgccgtcc agcttaatca tgatcgccac
     2881 gggatttgcg tcggcaatct tctgcaattc accggcggga cgtggctgat ccggcttggc
     2941 gatcgtcggc gcgcctggcc cctgtgatac gcgcagcggg cggctccaaa agaaccaccc
     3001 attgatcaag agagcaagga cgcaggcagc ggccgcgatg gctaccagaa tccagggccg
     3061 cgtcgatcgc cgcgagctgc tcccatttcg accgagactg tcgattctca gagaataagc
     3121 gcggcgtgca tcaccaccgg tagagctcgg atcgaacgcc cgaagatcgg aaggatc
//
LOCUS       DATAQL010000556         5396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_817443, whole genome shotgun sequence.
ACCESSION   DATAQL010000556 DATAQL010000000
VERSION     DATAQL010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817443"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VHS97_09625"
     CDS             <1..608
                     /locus_tag="VHS97_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX3448501.1"
                     /translation="SGQISAGALAHAVRNNRIKTVLNLRGSNSGRSWYRDELTTTLSA
                     GATHIDIAMSSCLWMSRTQLRALVDTLDTAECPILIHCAWGSERTGLAAAFSELLRPG
                     STLDDARAQFTVWYLFVPVGDGKVMAEHLDLYESWLRQERVEHTPANFRLWAHSGFQP
                     RHPSREDWRHDPHPPVVIRRPDSSPQAEPVASGVSASPAWK"
     gene            663..1043
                     /locus_tag="VHS97_09630"
     CDS             663..1043
                     /locus_tag="VHS97_09630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX3448502.1"
                     /translation="MVLSQDVTVRVRYAETDRMGLLHHANYFVYFEMARTELLRHRGI
                     TYREIEDAGHFLVIVDIDCKFKRPAHYDDLLTVRTTVERVTHVKIVHRYEVLRDGLLL
                     AEGHSTLACVDRDGKPQALPELLR"
     gene            complement(1072..2121)
                     /locus_tag="VHS97_09635"
     CDS             complement(1072..2121)
                     /locus_tag="VHS97_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3448503.1"
                     /translation="MPALLTSCPTNHCDQTRVRRIAARVFFLLVLGLSIGTVRSSPAR
                     EPEPNQSASTLESRVIPLIRAHKGKVAVAAKHLVTGESFVYHATEVMPTASLIKFPVM
                     IEAYRQAAAKQIDLDRVIVLRKQDKVPGSGVLTYHFTDGSTFKLRDAVRLMIVFSDNT
                     ATNLVLDAVGIGATAATMNKLGYANTKIHSKVFRRDTTVFPERSKQFGLGSTTADEMI
                     RLCQALYRKELVNAVACDEMTDHLRACDDKDKFPRFLPPGVKVAFKTGSLDDTRNAAG
                     IIEWSKGPVALCVLSCENEDKRWVADNAGNRLCAEIARAVYDHFDQTKPVPADIGSTN
                     ERGGRARDGSASPTP"
     gene            2401..3402
                     /locus_tag="VHS97_09640"
     CDS             2401..3402
                     /locus_tag="VHS97_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX3448504.1"
                     /translation="MKRLLVLSAFFAVAARADEPLPRIAEKLVRSGDEIVVCGQLFHT
                     TTKVVLWTDPGGYDAYRVVPRFGPDGGPVDRKTKPDLTKAGPGWRSHYGMRRGGLSPQ
                     EIEQVRGGWDVPLLQRVVDQFVIHFDAVGTSRGCFQVLQDERGLSVHFMLDLDGTIYQ
                     TLDLKESAWHATIANGRSIGIEVANIGAYHLNDRGRIDRWYKPGPDGKIRIVDPGTSQ
                     PLQLNSSAFELRPSRDDLIVGTIQGQELQQYDFTQQQYEALAKLTATLCTIFPKIRCD
                     YPRDAAGALRREKLPDPDFETYRGILGHYHVQTNKVDPGPAFQWDRLIDSSRKLMAR"
     gene            complement(3415..4818)
                     /locus_tag="VHS97_09645"
     CDS             complement(3415..4818)
                     /locus_tag="VHS97_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX3448505.1"
                     /translation="MIAHVPLRPVTTKSADSTAQQKKTQQGQGGGGGGGGGRGGRGGG
                     RGGRGGGGGGGMMGEDEKPGSTRILEIVPEGTQVKAGDIVAKLDGSTYEDEEQAQRIR
                     YLSAKAYVEQANAILEVNQITLREYRDGIYPQDMQLVRNYIQTCQLEKDRLTRTVNWS
                     RDMRKKGFRTQFQVHGDELALEQAEIALREAEGMLDRLAKQTGPKIIKSLEANVRAIQ
                     SDKLTQDASFSLEDQRLRRIRKNIENCTVRAPGEGIVVYVNQTDRWGQVTAPIDEGVT
                     LRQDQPIFSLPDPLHMRVKARINESKVTMIHSGQPAQIVIDAYPDRPLRGTVGEVTAI
                     NTPLNASDVRVYYANVSIDKGFENLRPGLSAEVLFRIDARRSVTRVPIESVRWVDEKA
                     YVARYDRARAEAGVEPWRWRQVELGLSDSQHAEVLSGLEVGDRVVSSPRTLPTPTRAT
                     IDPPPTRVAGLTHANSL"
     gene            4865..5209
                     /locus_tag="VHS97_09650"
     CDS             4865..5209
                     /locus_tag="VHS97_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448506.1"
                     /translation="MPDDPDPEPDPPDPPDPPDPPDPEDPPEPEPPPEDIPPDAPDDE
                     ELPPLELEEDNEEDDEVDEVDDDPPLFVLPDFEEDDDEPVFLVLDLLLLLVLLLASWL
                     WPLPLPPVPDPL"
BASE COUNT         1034 a         1623 c         1600 g         1139 t
ORIGIN      
        1 gttccggcca gatatccgca ggggcgctcg ctcacgccgt gcgcaataac cggatcaaga
       61 ccgttctcaa cctcaggggg tcgaattcag ggcgaagctg gtatcgtgac gagttgacca
      121 cgaccctgag cgcgggagca acgcacatcg acatcgccat gtcatcatgc ctgtggatgt
      181 ctcgcaccca acttcgagcg ctcgtcgata cacttgacac ggccgagtgc ccaattttga
      241 ttcattgcgc atgggggtca gaacgcacgg ggctggctgc ggcattttcc gagctcttga
      301 ggcccggcag cacgctcgat gacgcgcggg cccagttcac tgtctggtac ctattcgtac
      361 ctgtcggcga cggcaaagtc atggccgaac atctcgacct gtacgagagc tggctgcggc
      421 aagaacgtgt cgaacacact cccgcgaatt ttcgcctctg ggcgcacagc ggatttcagc
      481 cgcggcaccc gagccgcgaa gactggcgcc atgatcccca tccgccggtt gtgatccgaa
      541 ggcccgactc gtcgccccag gccgaaccgg tcgcgagcgg agtaagcgcg agccccgctt
      601 ggaagtgaga acttcccgat gtccggattc ttccagtcgg acgcgaatgg agcaaattcc
      661 tcatggtatt gagccaagac gttacggtgc gggtgcgata cgcggagaca gaccgcatgg
      721 gccttctgca ccatgcgaat tacttcgtct atttcgagat ggcccgcacc gagcttctcc
      781 gccaccgcgg aattacatat cgcgaaatcg aggacgctgg tcatttcctg gtcatcgtcg
      841 atatcgattg caaattcaaa cggcccgccc actacgacga tctcttgacc gtgcgcacaa
      901 ccgtggagcg ggtgacgcat gtcaagatcg tgcatcgcta cgaagtttta agagacggcc
      961 tgcttctggc cgaagggcac tcaacgttgg cctgcgtcga ccgcgacggc aaaccgcaag
     1021 ccctgccaga gttgctgaga taacaacact gcctgaaact gcgggttcaa ttcagggtgt
     1081 tgggctggcg cttccgtctc gcgcgcggcc gccgcgctcg ttcgtactgc caatgtcggc
     1141 cggaacgggc ttggtttgat caaaatgatc gtagacagct cgcgcgatct cggcgcagag
     1201 ccggttgccg gcgttatcgg cgacccagcg cttatcttcg ttctcacacg aaagcacaca
     1261 gagggcgacc ggccccttgg accattcgat gatccccgcg gcgttccggg tgtcgtcgag
     1321 gctgcccgtc ttgaacgcga ccttgacgcc cggtggcaga aagcggggga atttgtcctt
     1381 gtcatcgcag gctctcagat ggtcagtcat ctcgtcgcaa gcgacggcgt tcacaagctc
     1441 tttgcgatag agggcctggc acagccggat catttcatca gcagtcgtac tgccgagccc
     1501 gaactgctta ctgcgctcgg gaaagacggt cgtatcgcgg cgaaacacct tggaatggat
     1561 cttggtgttg gcgtatccga gcttattcat cgtcgcggcg gtcgccccga tgccaacggc
     1621 gtccagcacg aggttggtcg ccgtgttgtc ggagaagacg atcatgaggc ggacggcatc
     1681 acgcagcttg aaggtcgagc cgtcggtgaa atggtaggtg agcacacccg aaccgggaac
     1741 cttgtcttgc tttcgcaaaa cgatgactct atcgaggtcg atttgcttgg cggcggcctg
     1801 acgatacgct tcgatcatga cgggaaactt gatcaagctc gcggtcggca tgacctcggt
     1861 ggcgtgatag acgaatgact cgccggtaac taaatgcttg gccgctacag cgaccttgcc
     1921 tttgtgagca cggatcaagg gaatgacgcg cgactcaagg gttgacgcgg actgattggg
     1981 ctcgggctcg cgcgccggcg aagaacggac cgtgccgatc gaaagcccga gtacgagcaa
     2041 gaaaaagacg cgcgcagcga tccggcgtac tcgggtctgg tcgcaatgat tggttgggca
     2101 tgaagtcagt aaggcgggca tcttgatttg ctctcggggg tcgaagcgat tgggtcgaca
     2161 agcctgattc aacactgatt atcgcacgcg cgagcgcgaa ctccaatcag cactttacca
     2221 ttcagccccg ccatccgttc gacttgcata tcgaagcccg gcgcgggacg atttcaaatc
     2281 gccgcagtgt atgccaaaag aacatctgcg gcccaagggg ctccgcgacg ggattcttcg
     2341 gctgtgaccg ggtaggcgaa aacatattat cccgatcgcg taatgaggac ggttgagaca
     2401 atgaaacgat tgttagtgtt gtcggccttt ttcgctgtcg cggcccgcgc ggacgaaccg
     2461 ctacccagga ttgcggaaaa gctggtgcgt tctggtgatg agatcgtggt ctgcgggcag
     2521 cttttccata ccaccaccaa ggtggttttg tggacggacc ctggcggcta cgacgcatat
     2581 cgggttgtgc cgcggtttgg tccggacggc gggcccgtcg accgcaagac caagcccgac
     2641 ctgacgaaag ccggtccagg atggcgcagc cactatggaa tgcggcgggg cggactttca
     2701 ccgcaggaaa ttgagcaagt gagaggcggt tgggacgttc ctttacttca gcgggtcgtt
     2761 gaccagtttg tgattcactt cgacgccgtc ggcaccagtc gagggtgctt tcaggtcctt
     2821 caagacgagc gcggtcttag cgtccatttt atgctcgacc tcgacggaac gatttatcaa
     2881 accctcgacc tcaaagaatc ggcctggcat gccacgatcg ccaatggccg atcgatcggt
     2941 atcgaggtcg ccaatatcgg agcttaccac ctcaacgacc gcggtcgcat cgatcgatgg
     3001 tacaagccgg gtccggacgg caagattcgc attgtcgatc cgggtacgtc gcaacctctg
     3061 cagcttaata gcagcgcctt cgagctgagg ccgagccgcg atgatctgat cgtgggtacc
     3121 attcaaggtc aggaactgca gcaatacgac tttacacagc agcagtacga ggcgctcgcc
     3181 aagctgacgg cgactctgtg tacgattttt cccaagattc gttgcgacta tcctcgtgac
     3241 gctgcgggcg cgcttcgccg agagaaactg cccgatccgg actttgaaac ctatcggggt
     3301 attttggggc actatcacgt gcagacgaac aaggtcgatc cgggtccggc ctttcagtgg
     3361 gatcgactga tcgattcgtc tcgcaagcta atggcgcgct gagctacggt cttgtcacag
     3421 cgagtttgcg tgagtgagcc cggccacgcg cgtcggtggc ggatcaatgg tggcgcgtgt
     3481 cggcgtaggg agcgttcgcg gggaggagac cacgcgatcg ccgacttcga gcccgctcaa
     3541 gacttccgcg tgttgtgagt cgctcaggcc gagttcgacc tgccgccacc gccagggctc
     3601 tacgccggct tcggcgcgcg cgcgatcata tcgagcgaca taggccttct cgtcgaccca
     3661 ccggaccgat tcgattggca cccgggtcac ggaacggcgt gcatcaattc ggaacaacac
     3721 ttcggcactg agcccaggcc gcagattttc gaagccttta tcgatgctga cgttggcgta
     3781 atagacgcgc acatccgagg cgttcaaggg cgtattgatc gcagtgacct cgccgacggt
     3841 gccccgtagc ggccgatcgg gatacgcatc aatcacgatc tgcgccggtt gacccgagtg
     3901 gatcatcgtc accttggatt cattaatccg cgccttgacg cgcatgtgga gaggatcggg
     3961 caggctgaag atcggctgat cctgccgcag cgtcactccc tcgtcgattg gcgcggtgac
     4021 ctgcccccac cggtcggtct ggttcacata gaccacaatt ccctcaccgg gtgcgcggac
     4081 tgtgcagttt tcgatgttct tccgaattcg cctcaaccgc tgatcctcga ggctgaagga
     4141 ggcgtcttgc gtgagcttgt cggactgaat cgctctcaca ttggcctcga gagacttgat
     4201 gatcttgggg ccagtctgct tggccagtcg gtcaagcatg ccctcggctt cgcggagggc
     4261 gatctccgcc tgctctagcg ccagctcgtc cccatgaacc tgaaactgcg tgcggaaacc
     4321 tttcttccgc atgtcgcgcg accaatttac ggtgcgcgta agccggtctt tttccagctg
     4381 acaagtctgg atgtaattgc ggaccaattg catgtcttgc ggatagatgc cgtcgcgata
     4441 ttccctgagg gtgatctggt tgacttcaag gatcgcattg gcctgttcga catacgcctt
     4501 ggccgaaaga tagcgaatcc gttgagcctg ctcttcgtct tcgtaggtgg atccgtcgag
     4561 cttggcgacg atatcgccgg ccttgacctg tgttccttcc gggacaatct cgagaatgcg
     4621 ggtagagcct ggcttttcgt cctcacccat catcccgccg cctccaccac cgcctcttcc
     4681 tccccgaccg ccgccccgtc cgccgcgacc gccaccacca ccgccgccgc caccctgccc
     4741 ctgctgggtc tttttctgct gggctgtcga gtcggctgac ttggtcgtta caggacgcaa
     4801 ggggacgtga gcgatcacct cgtagctaaa gctgcggatt gttggcttta tagatgttgt
     4861 cgttgtgccc gacgatcccg atcccgaacc cgatcccccg gatcctccgg atcccccaga
     4921 tccccccgac ccagaagatc ctcccgagcc tgagccgccg ccggaggaca taccaccgga
     4981 tgcgcccgat gacgaagagc ttccgccgct tgaactcgaa gaagacaacg aggaagacga
     5041 cgaggtagac gaggtggacg atgatccacc actctttgtg cttcccgact ttgaagaaga
     5101 cgacgacgag cctgttttct tggtcttgga tttactcttg ctcttggtct tgctgctcgc
     5161 gtcctggctt tggccgttgc cgctcccgcc ggttcccgac ccgctctgag tgccgctggc
     5221 gctcccggag cccgatcctt ggctggatga tgagccgctt cctgtcgtgc cgctcccctt
     5281 ggaagtccct ccctgagtgc cgccaacggt tccgatcaag gcttcgactt ggcagcgcac
     5341 agtggtgtta ttcgcactct ccaccgtccc ttgctctacg acgacaatgt cgacat
//
LOCUS       DATAQL010000557         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3413249, whole genome shotgun sequence.
ACCESSION   DATAQL010000557 DATAQL010000000
VERSION     DATAQL010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3413249"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VHS97_09655"
     CDS             <1..710
                     /locus_tag="VHS97_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX3448507.1"
                     /translation="YTCLRELSAASRGGRRLMTLEDPIEMLVPGVAQAQINQAAGLDL
                     AVGLRSMMRQDPEVIGVGEIRDLATAEVALQASLTGHLLLCTFHAGSAAGVIGRLAEM
                     GIEPYVIISGVRAIVCQRLVRRLCTCARATADPADLLGLDVSNAWVTVGCTRCRGTGY
                     RGRVMLAEILVPNHQRIRRAIRAHADVGRLEEMAFRSGMVRLSERARYAVQSGLTDPV
                     EFRRVLGISSSPIPTSD"
     gene            919..1347
                     /locus_tag="VHS97_09660"
     CDS             919..1347
                     /locus_tag="VHS97_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3448508.1"
                     /translation="MRFMMLMIPKGYESASPGTVPDAKAVEAMMKYNESLQKAGVLLA
                     LDGLHPPSMGARVSFAGGKPKVTDGPFTEAKEVVGGYWMIQVKSKEEAIEWASRCPGS
                     ENEVIEVRQVQEFSDFPTDVQEAVAKFPEMQAPAGRPSGT"
     gene            1409..2695
                     /locus_tag="VHS97_09665"
     CDS             1409..2695
                     /locus_tag="VHS97_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3448509.1"
                     /translation="MSTADTHRTIDAVWRIESARLVAGLARIVRDVGLAEELAQDALV
                     AALEQWPESGVPHNPGAWLMATAKHRAIDHVRRNKRLARKHQELAHRLEIEQENAATA
                     LDAVLDDDVGDDLLRLVFTACHPVLSTEARVALTLRLLGGLTTEEIARAFLVSEATVA
                     QRIVRAKRLLVEKNVAIEVPRGAELSARLSSVLGVIYLVFNEGYTATAGDDWMRPALC
                     EEALRLGRILAELAPAEPEVHGLVALMEIQASRLRARVGPSGEPIVLFDQNRARWDQL
                     LIRRGLAALARAEQLGGAPGPYVLQAAMSACHARARTPEDTDWARIARLYAELAKLTP
                     SPVVELNRAVAVAMADGPQAGLELVDSLTTEPSLQGYHLLPTVRGDFLAKLGRVDEAC
                     EEFKRAASLTRNARERELLLERARTCGGRSILPVSE"
     gene            complement(2797..2928)
                     /locus_tag="VHS97_09670"
     CDS             complement(2797..2928)
                     /locus_tag="VHS97_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448510.1"
                     /translation="MTKQRKQAYPAGWDEERIRKLAERYDNQTEDEQVAEHKAALLA"
BASE COUNT          564 a         1030 c         1005 g          607 t
ORIGIN      
        1 tctacacgtg cctgcgcgag ctctcggccg cctcgcgcgg gggccggaga ctcatgacgc
       61 tggaagaccc gatcgagatg ctcgtcccgg gcgtggccca ggcccagatc aaccaggcgg
      121 ccgggctgga cctggcggtc ggcctgcgct cgatgatgcg gcaggacccc gaggtgatcg
      181 gagtgggcga gattcgcgac ctggcaaccg ccgaggtcgc actccaggcg tccctcaccg
      241 gacatctttt actgtgcaca ttccacgccg gcagtgcggc cggtgtcatc ggccgcctgg
      301 ccgagatggg aatcgagcca tacgtgatca taagcggtgt ccgcgccatc gtctgccagc
      361 gcctggtgcg acggctttgc acgtgcgccc gcgccaccgc cgacccggca gacctgctgg
      421 gactggacgt ctcgaatgct tgggttaccg tggggtgcac gcggtgccgt ggcaccggct
      481 accgcggccg cgtcatgctg gcagagatcc tcgtgccaaa ccaccagagg atcagacggg
      541 ccatccgcgc ccatgccgac gtcggccgct tggaggagat ggcctttcgt tcgggcatgg
      601 tcaggctgtc ggagcgcgcc cgctatgcgg tccagtccgg gctaactgat ccggtcgagt
      661 ttcggcgtgt gctcgggatc tcgtcctccc cgatcccgac ctcagactga cacgctcgtg
      721 cccgggagcg tcggggcatc ccgcgccgaa ccatcgacct cagatatacg aaaccaggag
      781 atcctcagtc ttcgttaaca tttccggaac gtgtcgatct ggctttcagt cgttcgacgc
      841 gtacatagcg gctcggtcta gacggccgag atcgtcacaa tttcagccta atcagatgac
      901 gcttacggag acacaagaat gcgctttatg atgctgatga ttcccaaggg ctatgagagt
      961 gcctcgcctg gtaccgtgcc ggacgccaag gccgtcgagg cgatgatgaa gtacaacgaa
     1021 tccttgcaaa aagccggcgt tctgcttgcg ctcgacggcc tccacccccc ctcgatgggt
     1081 gcgcgagtct cattcgctgg tgggaagccc aaggtcaccg acgggccctt cacagaggcc
     1141 aaggaggtcg tgggcggcta ctggatgatt caggtgaagt caaaggaaga agcgatcgaa
     1201 tgggcatcgc gctgcccagg ttcagagaat gaagtaattg aggtacgtca ggtccaggag
     1261 ttctccgact ttcccaccga tgtccaggag gccgtggcca agtttcccga gatgcaggcg
     1321 ccggccggac ggcccagcgg aacgtaacgc cggagtcgcc gcggtgttgg cttgcaccgg
     1381 cgcgccgaag atgctttgat cggttgtcat gtcgacagcc gatacacatc gcactatcga
     1441 cgcggtctgg aggatcgagt cggccaggct tgttgccggc cttgcgcgaa tcgtgcgcga
     1501 tgtcggactg gccgaggagc tcgcgcagga tgccctcgtc gccgcgctcg agcaatggcc
     1561 agagtcaggc gtcccgcaca atccaggtgc ctggctcatg gccacggcaa agcatcgggc
     1621 aatcgatcac gtgcggcgaa acaagcggct cgctcgcaaa catcaggagc tcgcacacag
     1681 gcttgagatc gaacaagaga atgccgcaac ggcacttgat gcggtcctcg acgacgacgt
     1741 gggcgacgac ctcctgcgtc tcgtgttcac ggcctgccat cccgtgctct ccaccgaggc
     1801 gcgcgtcgcg ctcacgcttc gtttgctcgg cggtctgacg accgaggaga tcgcgcgggc
     1861 gttcctcgta tccgaggcca cggtcgccca gcggatcgtc cgagcgaagc gcttgcttgt
     1921 cgaaaaaaac gttgccatcg aggttccgcg cggggccgaa ctttccgccc gcctctcatc
     1981 ggtgctcgga gtcatctacc tggtcttcaa cgagggctac acggcgaccg ctggcgacga
     2041 ctggatgcgg cccgcgctct gcgaggaggc gctgcgtctt gggcgtatcc tggccgagct
     2101 tgccccggcc gagcccgagg tccacggact tgtcgcgctc atggaaatcc aggcatcgcg
     2161 gttgagggcg cgcgtgggac catcaggaga gcccattgtc ctcttcgatc aaaaccgcgc
     2221 tcgttgggat caactcttga tccgtcgcgg tctggccgcc cttgcgcgtg ccgagcaact
     2281 cggtggggcg cctggcccgt acgtactcca ggcagcgatg tctgcatgcc atgctcgagc
     2341 tcgcacccca gaggatacgg actgggcgcg gatcgcacga ctctacgccg agctcgctaa
     2401 gctcacgccg tcgcccgtcg tcgagctgaa ccgtgcggtg gcagtcgcca tggccgacgg
     2461 tccccaagcc ggcctcgagc tcgtcgattc gctgactacc gagccatcgc tgcagggcta
     2521 ccacctcttg ccgaccgtgc gtggcgactt tctcgccaag cttggccgag tcgacgaggc
     2581 atgcgaggag ttcaagcgcg ccgcttccct tacgcgtaac gctcgagagc gcgagcttct
     2641 cctggagcgc gcccgaacat gtggagggcg ctcgatacta ccagtgtcag aatgacaaaa
     2701 tcgttgcgca gtgcagccct atgctggccg tttcttgctt atgagcttga caatctccgg
     2761 gcccagttcg gagggcacaa ccatgacggt ctgcccctag gccaacaaag ccgctttgtg
     2821 ttcggcaacc tgctcgtctt cggtttggtt atcatagcgc tccgcaagtt tgcggatacg
     2881 ctcttcatcc catccggcgg ggtacgcctg ttttctttgc ttcgtcattg ccgcttcctt
     2941 cgttggcgtc tgcgaaacgc ttgcttcgcc ttgcgcgtca ggtcgaaggc ggtgacaacg
     3001 aatacaccgt cgccaacaga atccggcacg ttaaccactt gcaaataccg tcccgccgca
     3061 gtctgaccga gccgaattcg cgaacgatca ctactgcgag tcctgcggtc ggcccttagc
     3121 cgccggcaat agccccaacg atcaagaggg gttccttccc agatgcgacc gtgtcgggta
     3181 acggtgtgtc cggcggctca tgggac
//
LOCUS       DATAQL010000558         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_208617, whole genome shotgun sequence.
ACCESSION   DATAQL010000558 DATAQL010000000
VERSION     DATAQL010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208617"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..1387)
                     /locus_tag="VHS97_09675"
     CDS             complement(380..1387)
                     /locus_tag="VHS97_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3448511.1"
                     /translation="MARPVTLFTGQWADLTLEVIAEKASRWGYDGIELPCWGDHFNVQ
                     KALNEAIYCKGRHELLAKHGLKCWAISNHLTGQLVLDHNDARTDDWVPASIRGDAKKK
                     TEWAIQEMKDTARAAQRLGVKVVNGFTGSSIWHLLYSFPPVSDATIDEGFKLFADRWN
                     PILDVFKECDVKFALEVHPTEIAFDIYTAERALKAVNHRPEFGFNFDPSHLLWQMIDP
                     VEFIRAFPDRIYHVHVKDAARTLDGRTGILASHLNFGDPRRGWDFRSPGRGQVNFEEI
                     ARALNHVGYEGPLSVEWEDCGMDREYGAAEAARFVKEKMGFKSAGRLFDSAFAEAQAK
                     E"
     gene            1637..2287
                     /locus_tag="VHS97_09680"
     CDS             1637..2287
                     /locus_tag="VHS97_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX3448512.1"
                     /translation="MPPKAILFDFDGVIADTDNHHIAAWQRTLAVMGWQVAEEVAAQS
                     AEIDDRDFLAQLFTHRGITGANIDEWVRRKQALAVQLLKDAPRLFPGVVDLVHSLQGR
                     ARLAVVSGTWRENIQAVLESAGLFGSFDTIVGREDVTGRKPAPDPYQLALKRLRLVAR
                     STVALEDSPAGLASARAAGIRPIAIGHRHLFGEWVGDATYVSGFEPAEGLLQHLGL"
     gene            complement(2307..2867)
                     /locus_tag="VHS97_09685"
     CDS             complement(2307..2867)
                     /locus_tag="VHS97_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3448513.1"
                     /translation="MSSLTQRAATLDDLYRVDGKAELIGGRIVPLMPTGRRPNRVPAR
                     IYRSLDDFAQATGRGEAYTDNMGFAVHRLASGRESFSPDASYFLGPFPLNEMRFLNGP
                     PVFAVEVRSENDYGDKADLARAAKRADYFEAGTGVVWDVDPVNDRILKYAGDAPDHPT
                     VFVKGQIADAEPAVPNWTIAVDSIFA"
     gene            complement(2990..>3769)
                     /locus_tag="VHS97_09690"
     CDS             complement(2990..>3769)
                     /locus_tag="VHS97_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3448514.1"
                     /translation="GGPHAPTVSDAWDGGRMWDQALAREGFVVFLMDPRSASGKGAGS
                     AWKAYKHLGVQELEDIKDAINWLKQKPYVDGSRIGMAGHSYGGYITSYAMTHSDLFAA
                     GIAGAPVTDWRDYDSIYTERYMGLPQENPEGYNVSSVVKAAGKLHGKLLILHGAIDDN
                     VSLRNTMRLVEALEGANKDFELMIYPSSRHGIRGPHYARLQLDFIRRTLGGMKPEKPT
                     SPGDLAERVKEDTPARGARAGTSEGARRPAGERAHGENGSN"
BASE COUNT          714 a         1147 c         1133 g          775 t
ORIGIN      
        1 agggttcgcc gtttccgccg gctcggctga cgcatcctcg tcgtcactgc catgctcgag
       61 ggggcgctcg ccgaaaatct tgtcaaaatt cgggaagcca tacttccgga cgatcgggcc
      121 gctcatggtg gctgtctccc aggtattggc gtcgcaggca tttgggccga tcgctgctga
      181 ctgccggctc tctcaatcgt catttaccat gtattttaac ctatacatgg ggatacgtcc
      241 agtcgcgtat acagaatctg caccatggtt tttccgggaa atcagggggt gaggatcgcg
      301 cggcgcggag gacagacccg acgttgaaga atcaaaatca agctgttgac ccgctttccg
      361 aggcgacggg gcgatcccct cattccttgg cctgcgcttc agcaaaggcc gagtcgaaga
      421 gccgcccggc cgacttgaag cccattttct ccttgacgaa tcgcgccgcc tcggcggctc
      481 cgtactcgcg atccataccg caatcttccc attcgaccga gagcggcccc tcgtagccga
      541 cgtggttgag cgctcgagcg atctcctcga agttgacctg tccgcggccc ggtgagcgaa
      601 agtcccagcc ccggcgcggg tcgccgaaat tgagatgcga cgccaggatg ccggtccggc
      661 cgtcgagcgt ccgggctgcg tccttgacgt gcacgtgata gatccgatcg ggaaaagccc
      721 ggatgaattc gacggggtcg atcatctgcc agagcaggtg gctcggatcg aagttaaacc
      781 cgaactcagg acgatgattt accgctttca aggcgcgctc ggcggtgtag atatcaaagg
      841 cgatctcggt gggatggacc tcgagggcaa acttgacgtc gcattccttg aagacgtcga
      901 ggatcgggtt ccagcgatcg gcgaacagct tgaatccctc atcgattgtg gcgtctgaca
      961 ccggtgggaa tgagtagaga agatgccaaa tggaagagcc ggtgaagccg ttgaccacct
     1021 taacaccgag tcgctgggcg gctcgcgcag tgtctttcat ttcctggatg gcccactcgg
     1081 tcttcttttt ggcgtcgccc cgaatcgatg ccggtaccca gtcgtcagtg cgggcgtcgt
     1141 tatgatcgag cacgagctgg ccggtcagat gattggagat cgcccagcat ttgagcccgt
     1201 gtttggccaa gagctcatga cgccccttgc aatagatcgc ctcgttcaag gctttctgca
     1261 cgttgaaatg gtcgccccag catggcagct cgataccgtc ataaccccat cggctcgcct
     1321 tctcggcgat aacttccagc gtcaggtcgg cccactggcc ggtaaatagt gtgacgggtc
     1381 gtgccatggt gagttctccc agtcttagtg atcgtcaaag gggtggacag acggcgtccg
     1441 cgggtcgatc gcggccgcgg gcgggatagc gatgggaccg aagggttcac acgtctccaa
     1501 agccactgtg ggctcgaaat cgaaagatag cgctggcggc tgaacttgtc aatgatttga
     1561 cggttagact cccctttcgg gataatgccg gggaaagcat ccggcccttg tttgattggc
     1621 ggaatgacaa cgacacatgc ctccgaaggc catcctcttc gatttcgacg gcgtgatcgc
     1681 cgacaccgac aaccatcaca tcgctgcctg gcaacggacg cttgcggtca tgggatggca
     1741 ggttgccgag gaagtggcgg ctcaatcggc cgagattgac gaccgtgatt tcctggccca
     1801 gctcttcacg caccgcggaa tcacgggggc gaatatcgac gaatgggttc gccgcaagca
     1861 ggcactcgcc gtgcaactgc tcaaggatgc tccgcggctt tttcctggag tcgttgatct
     1921 ggtccacagc ctgcaaggac gggctcgcct ggcggtcgtc tcgggcacct ggcgtgagaa
     1981 tatccaggcg gtcctggagt ccgccggtct cttcggctcg ttcgatacga tcgtcggcag
     2041 ggaagatgtg acggggcgca agccggctcc cgacccctat cagctggcgc tcaagcggct
     2101 caggctggtt gccagatcga ccgtcgcgct cgaggattcg ccggccgggc tggcttcggc
     2161 ccgcgccgcg gggatccgcc cgatcgcgat cggccaccgc catctgttcg gcgaatgggt
     2221 cggcgacgcc acgtacgttt cgggcttcga accggctgaa ggattgctgc aacacttggg
     2281 attgtgaggt aatcgacaac atcgggtcac gcgaagatcg agtcaacggc gatagtccaa
     2341 ttcggaacgg caggttccgc gtcggctatt tggcctttca cgaagacagt aggatggtct
     2401 ggtgcatcgc cggcatattt caggatgcga tcgttcacgg gatccacgtc ccagacgacg
     2461 cccgtacccg cctcaaaata gtccgctcgc ttggctgctc gggccaggtc ggctttatct
     2521 ccgtagtcgt tctcgctgcg gacttccacc gcgaagacgg gtggcccgtt gagaaaccgc
     2581 atttcgttca atgggaacgg gccaaggaaa taggacgcgt cgggcgaaaa cgactcgcgc
     2641 cccgacgcga gtcgatgaac cgcgaaaccc atgttgtcgg tgtatgcctc gcctcgcccg
     2701 gttgcctggg caaaatcgtc cagactgcga taaatgcgag ccggtacacg gtttggccgg
     2761 cgccctgtgg gcatgagcgg aacaatcctt cccccgatca actccgcttt tccgtccacg
     2821 cgatacaggt cgtcaagcgt ggcagctcgt tgagtcaatg acgacatgcg gaacctctcg
     2881 tcaaagcctg cactcaaaaa atcagggacg cggcctcatg tgaatcgtac cggccatcga
     2941 atctgccggc aatttgtaat cacttcgctg ggatcgccaa cgtccgcagt cagttcgatc
     3001 cattctcacc gtgcgcgcgt tcgccagctg gcctcctcgc gccctccgat gtacccgccc
     3061 gcgcccctcg ggcgggagtg tcttccttca cccgttccgc caggtcgccg ggcgaagtcg
     3121 gtttttctgg tttcatccct cctagtgtcc gccggatgaa atcgagctga agccgggcgt
     3181 aatgcgggcc gcggatcccg tggcgggatg acggataaat catcagctcg aagtctttat
     3241 tggccccttc gagcgcctcc accagcctca tcgtattccg tagcgagacg ttgtcgtcga
     3301 tcgcgccgtg caagatcaac agcttgccgt gcagcttgcc ggccgccttc acgaccgatg
     3361 agacgttgta gccctcgggg ttttcctgag gaagacccat ataacgctcc gtgtagatcg
     3421 agtcgtaatc gcgccaatcg gttacgggag cgcccgcgat cccggcggcg aacaaatcgg
     3481 aatgggtcat cgcgtacgac gtgatgtagc cgccatagct atgaccggcc atcccaatac
     3541 gcgagccgtc aacatagggc ttttgcttga gccagttaat cgcgtccttg atatcctcca
     3601 gctcttgaac tcccagatgc ttgtacgcct tccaggcgga acccgcccct ttgccactgg
     3661 cgctacgggg gtccatgaga aagacgacaa atccctcgcg ggccagcgcc tgatcccaca
     3721 tgcggccgcc gtcccaggcg tccgagaccg tcggggcatg aggcccgcc
//
LOCUS       DATAQL010000559         5080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5247934, whole genome shotgun sequence.
ACCESSION   DATAQL010000559 DATAQL010000000
VERSION     DATAQL010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5247934"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..2743
                     /locus_tag="VHS97_09695"
     CDS             173..2743
                     /locus_tag="VHS97_09695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEX3448515.1"
                     /translation="MAKRNRRGDREPGKGGKRPSAHIEDVPLAEAARSRYLNYALSVI
                     TSRALPDLRDGLKPVQRRILYAMWHDLHVTADGRYIKCAAVVGEVMKTYHPHGDQSIY
                     DALVRMAQPFSLRQTLVDGYGNFGSIDGDPPAAMRYTECRLTPIAHALLSELREQTVD
                     FRPNYASTAEEPIVLPAQFPNLLVNGVSGIAVGMATNIPPHNLKEVCTALVALLDNRE
                     LALEKLLKSIHGPDFPTGGVILNSADEIRQIYATGQGTIKLRGTYEPHPERANSVLIT
                     SIPYGIEKDALVERIGEMIAKGQVPQLTNVKDLSTDDVRILLELKPGANPDAALAYLF
                     KNTPLQINYSINLTCLLPAEGAQVAVPARLDLKSILQQFLDFRLEVVTRRLRFELKNL
                     LDRIHILEGFAIVFKNLDEAIKIIRASDGKADAAPKLISRFALSELQADAVLETKLYR
                     LGKLEIRDILDELAQKRKRALEIQQLLDDEPARWQIIRGELKEIAQKFGEPRRSRVEV
                     PAAPLEFREEDYIVDEDAWVIVTRDGWTKRQKSFTDAASIRVRDNDRAGWIYRARARQ
                     TLTFFTDRGIAYTLRVNDIPMTTGHGEPIQKQFAFEDQEHIVGVICHDPRCLPDSSKY
                     PQTTPRLVQRLLGSDLDGPADDAPARGDNDHGAGEANGNGSMPSLPPPPYAVALTAGG
                     KVLRFAISSLAAVSTRKGRLVVRLDSSFKGDIVVGVEATDGSENVCLATRSARVLIFP
                     VTEANIVAGAARGVNAIRLESKDGVIGFVLANKKREGLTVRTNRGATQIVRATKYPVT
                     SRGGKGYAILQRGSLDAVIPDEAEPIPSLEEVGETPPLRNGTAEHREKNGEATDTGGE
                     "
     gene            2785..4707
                     /locus_tag="VHS97_09700"
     CDS             2785..4707
                     /locus_tag="VHS97_09700"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX3448516.1"
                     /translation="MSTGSYTAKSITVLEGLEAVRRRPGMYIGGVDKTGFHHLLWEIV
                     DNAVDEVMNGHATRIVVTLHSDGKTMSVADNGRGIPVDSHPKSGKSALEVIFTTLHAG
                     GKFDNDAYKVAGGLHGVGASVVNALSKSLIAEVRRDGHTFSQRYRRGKPLGPVEKGEP
                     ARGTGTTVTYTPDPDVFADLNYDPSLIAERLEIKTYLNKGLVIQFVDQKSKSNVEFRH
                     EGGVVDFLDAINTQRTDQRVAALPFVLEREDEQEGLRCHLALAWTEATDEDVRSFVNT
                     IPTRDGGTHELGMLAAVGAAVQRFMETHDLVRKGMEIKREDIREGLTAVLAVCVREPQ
                     FQGQTKGRLNNPEVKAQIESMTRPALELFLHKNKSVGDAIAARVIQSAKAREASRAAA
                     SQVRRKTAISGRLNLPGKLHDCDSTNPDESELFIVEGDSAGGSAKQGRDRDIQAILPL
                     KGKVLNAEQAGKAKVLDNKELTDIISALGCGMDDQFDPGRLRYGKIILLTDADSDGHH
                     IATLLLTFFYRHVPGLFADGRVYLACPPLYKVSWGKETFWASEDADRDRILAKLPKNA
                     KPNITRFKGLGEMPAALLFETTLDPTRRRLLRVVIPDDDRPYTDRTVSDLMGKEPEAR
                     FKFIMEEAYTAKDIDI"
     gene            4836..>5080
                     /locus_tag="VHS97_09705"
     CDS             4836..>5080
                     /locus_tag="VHS97_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3448517.1"
                     /translation="MSMIEAVGLTKTYRAVQKKEGVRGALRGLYHREYREVHAVEQIS
                     FTIEPGEMVAFLGPNGAGKTTTLKMLSGLIYPSSGEAR"
BASE COUNT         1064 a         1521 c         1549 g          946 t
ORIGIN      
        1 agccgtctcg ctggtgatct agtaggccgc gcttgcacga accgggcggg cgcgttatgg
       61 tatactaact tatagtgcct gtaagcagga tttggaaact gggattgttc ggtgagcgat
      121 ttctgggtct ttttgtgtaa acgaggttgt cttgatcggg tgagaggaag taatggcgaa
      181 gcggaaccgc cgaggtgatc gggaaccagg gaaggggggc aagcgcccca gcgcgcacat
      241 cgaggacgtg ccgttggcgg aagcggcgag gtcgcgctat ctcaactacg ccctctcggt
      301 gatcacgtcg cgagcgctgc ctgatttgcg tgatgggctc aagcccgtcc agcgtcgcat
      361 tttatatgcc atgtggcatg atttgcacgt cacggctgat ggtcgttata tcaagtgcgc
      421 cgcagtggtc ggcgaggtga tgaagactta tcacccgcat ggcgaccaat cgatctatga
      481 cgccctggtg cgaatggcgc agccgttcag cctgagacaa acgctggtcg atggctacgg
      541 caatttcggg tcgatcgacg gcgacccgcc ggcggccatg cgctacaccg aatgccggct
      601 gacgccgatc gctcatgcgc ttttgagcga attgcgagag cagaccgtcg atttccggcc
      661 caactatgcc tcgacagccg aggaaccgat cgtcctgcct gcccagttcc ccaacttgct
      721 ggtcaacggc gtttcgggga tcgccgttgg catggcgacc aatattcctc ctcacaactt
      781 gaaagaagta tgcaccgcct tagtcgcact acttgataat cgagagctgg cgcttgaaaa
      841 gctgctcaag tctattcatg gtcctgactt cccgacgggg ggcgtgatcc tcaattcggc
      901 cgatgagatc cgccagatct acgcgaccgg gcaggggacg atcaaactgc gcgggacgta
      961 cgagccgcac cccgagcgtg ccaactcggt gttgatcacc tcgattccct acggcatcga
     1021 gaaagacgcc ctggtcgagc ggatcggcga gatgattgcc aaggggcagg tcccacagtt
     1081 gacgaacgtc aaggatctct ccaccgacga cgtccggatc ttgctcgagc tcaaacccgg
     1141 tgccaatccc gacgcggccc tggcgtatct gttcaagaat actccgctgc agatcaacta
     1201 cagcatcaac ctcacgtgcc tcttgccggc cgagggcgcg caggtcgcgg ttcccgcccg
     1261 gctggatctg aagtcgatcc tccagcaatt cctcgacttt cggttagagg tcgtcacgcg
     1321 caggcttcgg ttcgagctca agaacttgct cgatcgaatc catatcctcg aagggttcgc
     1381 gattgtcttc aagaatctcg acgaggccat caagatcatt cgcgccagcg acggcaaggc
     1441 cgacgcggcc cccaagctga tcagtcgctt tgcgctttcc gaattgcagg ccgatgcggt
     1501 gctggaaacc aagctgtacc gcttgggcaa gctcgagatt cgcgatatct tggacgagct
     1561 cgctcagaag agaaagcggg cgcttgagat ccagcaattg cttgatgacg agccggctcg
     1621 atggcaaatc atccggggtg agctcaaaga gatcgcccag aagttcggcg agccgcggcg
     1681 cagccgcgtc gaggttcctg ccgcgccgct cgagttccgc gaggaagact acatcgtcga
     1741 cgaggatgcc tgggtgatcg tcacgcgcga cggctggacc aagcgccaga agtcgttcac
     1801 cgacgcggca agcatccgcg tccgggataa cgatcgcgcc ggctggatct atcgggcgcg
     1861 ggcccggcaa accctgactt ttttcaccga ccgcggtatt gcctacacgc tccgggtcaa
     1921 cgacatcccg atgacgaccg ggcatggcga gccaattcaa aagcaatttg cctttgaaga
     1981 ccaggaacac attgtcggtg tcatctgcca cgacccgcgc tgcctgcctg actcgagcaa
     2041 gtatccgcaa acgacgccgc gtctggtgca acgcctcctg ggaagcgact tggacggtcc
     2101 ggccgacgac gctcccgctc gcggcgacaa cgatcacggc gcaggcgagg ccaacggcaa
     2161 cggttctatg ccgagtctgc ctcctccccc ctacgcggtt gccttgaccg cgggaggcaa
     2221 ggtgttgcgg tttgcgattt cgtcactcgc cgccgtctcg acgcgcaagg gtcggctggt
     2281 cgtccgactc gactcatcgt tcaagggaga cattgttgta ggagtcgaag ctaccgatgg
     2341 gtcggaaaat gtctgcctgg caacccggtc ggcgcgggtg ctgatcttcc cggtcaccga
     2401 ggctaacatc gtcgccggcg cggcccgagg tgtaaacgcc atccgtctcg aatccaaaga
     2461 cggcgtcatc ggctttgttc tggccaacaa aaagcgcgag ggtctcaccg tacggaccaa
     2521 ccggggagcc acgcagatcg tgcgggcaac caagtatcca gtcacgtcgc gcggcggcaa
     2581 gggctatgcg attctccagc gcggctcgct cgacgcggtc atccccgatg aggccgaacc
     2641 gatcccgtcg ctcgaagaag taggtgagac gccgccttta cggaatggaa cagcggaaca
     2701 tcgggaaaaa aatggtgagg caaccgacac tggcggcgaa taagaaccgt tcaatgacgg
     2761 aatgattccg tcacggaagc gactatgtct accggatcgt acacagcaaa atcgatcacc
     2821 gtgctcgagg gcctcgaagc ggttcggcgc aggccgggca tgtatatcgg cggcgtcgac
     2881 aaaacgggct ttcatcacct gttgtgggaa atcgttgata acgccgtcga tgaggtgatg
     2941 aacggtcacg cgacccggat cgtcgtgacg ctgcattccg acggcaagac gatgtcggtc
     3001 gccgacaacg gccgcggcat cccggtcgac tcgcatccga agtcgggcaa aagcgcgctc
     3061 gaggtgatct ttacgacttt gcacgccggc ggcaagtttg acaacgatgc ttacaaggtc
     3121 gccggcggac tgcatggtgt gggcgccagc gtcgtcaacg cgctttcaaa gagcctgatc
     3181 gccgaggtgc ggcgggacgg ccatacgttc tctcagcggt accggcgcgg caagcccctg
     3241 gggcccgtcg aaaaagggga gccagcgcgt gggaccggca ccaccgttac ctacacgccc
     3301 gatcccgacg tgtttgccga tctcaattac gacccaagcc tgattgccga gcggctcgag
     3361 atcaagacct acctgaacaa ggggctggtc atccagtttg tggatcaaaa gagcaagtca
     3421 aacgtcgagt tccgccacga ggggggcgtt gtcgactttc tcgatgccat caatacccag
     3481 cgcaccgacc agcgggtcgc ggccttaccg tttgtgctcg agcgcgaaga cgagcaagag
     3541 ggtttgcgct gtcacctcgc actggcctgg accgaggcca ctgatgaaga cgtgcggtca
     3601 ttcgtcaata cgattccgac ccgcgacggc ggcacccacg agttggggat gctggccgcg
     3661 gtcggcgccg ccgtgcagcg gtttatggag acgcacgacc tggtcaggaa agggatggaa
     3721 atcaagcggg aggatatccg cgaaggcctg accgccgtcc tggccgtctg cgtccgcgag
     3781 ccgcagtttc aggggcagac caagggaagg ctcaataacc cggaagtcaa ggctcagatc
     3841 gagtcgatga ctcggccggc gctcgaactc ttcctgcaca agaacaagag cgtgggggac
     3901 gcgatcgcgg cccgcgtgat ccagtcggcg aaggctcgcg aggctagccg agctgccgct
     3961 tcgcaagttc gccgcaagac cgcaatcagc gggcggttga accttccggg caagctccac
     4021 gactgcgaca gcaccaaccc cgacgaatca gagctcttta tcgtcgaagg cgacagcgcc
     4081 ggcggttccg ccaaacaagg acgcgatcgc gatatccagg cgatccttcc cttgaagggc
     4141 aaggtcttga acgccgagca agcaggcaag gccaaggttc tcgacaacaa ggagctgacc
     4201 gatatcatca gtgcactcgg atgcggcatg gacgaccagt tcgaccccgg ccggctgcgc
     4261 tatggcaaga ttatcctcct gaccgatgcc gacagcgacg ggcaccacat cgccaccttg
     4321 ctgctgacat tcttttaccg gcacgtcccc ggcctcttcg ccgacgggcg tgtctatctg
     4381 gcgtgcccgc cgctctacaa ggtctcgtgg gggaaggaaa cgttctgggc gtccgaggat
     4441 gccgaccgcg accggatcct ggcaaagctt cccaagaacg ccaaacccaa tatcactcgc
     4501 ttcaaggggc tgggcgagat gccggcggct ctgctattcg aaacgacact ggacccgact
     4561 cgccgccgcc tacttcgcgt cgtgatcccc gacgacgatc gcccttacac cgaccgcacc
     4621 gtcagcgatc tgatgggcaa ggagcccgag gcccggttca agttcatcat ggaagaggcg
     4681 tacacggcca aggatatcga tatttgattt ctcttcactc tatttcgcaa tccggtcatt
     4741 tgatctggaa ttccagattc taaaactgat cttgccagtt ctgaggcgtc ctacgcccga
     4801 gcggcacggc ctgtgttcgc aaggaagatt agccaatgag catgattgaa gccgtgggtt
     4861 tgaccaagac ctatcgggct gttcagaaaa aggagggagt gcggggtgcc ttgcgcggat
     4921 tgtatcaccg tgaataccgc gaagttcacg ccgtcgaaca gatcagtttt acaatcgagc
     4981 ccggtgagat ggtggctttc ctgggaccaa atggcgcggg caagaccacg acgctcaaga
     5041 tgctctctgg cctcatttat ccgagtagcg gcgaagcgcg
//
LOCUS       DATAQL010000560         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2957322, whole genome shotgun sequence.
ACCESSION   DATAQL010000560 DATAQL010000000
VERSION     DATAQL010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2957322"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VHS97_09710"
     CDS             complement(<1..501)
                     /locus_tag="VHS97_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX3448518.1"
                     /translation="MRVRQRPWSQRYNIAVIALAVASATISMSASGGEPDSNSQAARE
                     HFFEQSVRPLLAENCYSCHGDKKQKGGLRLDSLEAILHGGESGPAVVPGKPTESLIVE
                     AVNFAGLEMPPTGKLSDDKVSVLTRWVSLGAPWPSRDRAAHAPSDLTKPTGSKFTPLD
                     RALWSFQ"
     gene            1034..1342
                     /locus_tag="VHS97_09715"
     CDS             1034..1342
                     /locus_tag="VHS97_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448519.1"
                     /translation="MRVVGRRIGLFHIVKAHRVFGESPAKIAEEFELPLELTAEVLAF
                     AEKHKVEVDAYVADYQAELDRQCAAYQPSPTPLRISRQVAERRAQESQPECSARPVAR
                     "
     gene            complement(1363..3669)
                     /locus_tag="VHS97_09720"
     CDS             complement(1363..3669)
                     /locus_tag="VHS97_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX3448520.1"
                     /translation="MQSGIVVLFALASFVGAFLLFVVQPMIGKMALPLFGGTSAVWNT
                     SLVFFQGTLLCGYLFTYGLGRTGWSLRGRAGAVYLLGFVASLALGYYMQPIVLEPNSD
                     RRSSSAGDPALVLLGVLLRSAALPLVLVSATAPLVQRSFALTGHRRADDPYFLYAASN
                     TGSLLALLIYPFVIEPNLGLTAQSQLWKTGFLILAALLVACVLLARWLNRSRPVGNSP
                     RLFDLHGAERRFALAIWLRWLALVFIPSSWLLGVTAYLTTDMASVPLAWIIPLALYLL
                     SFILAFARSAVAFVRLATRSLPFLIAPLVLVMSAGFVHVYWIPLHLIAFFAASVACHG
                     ALAQMRPETERLSLYYLTIALGGLLGGIWTAIAAPILFDRVVEYPLAIVLTCLAAPLT
                     KIRDWGRSPREWLGDFVFAGVVFVLAASLATNQGGLGDSVLGALALIVASGLGILACM
                     TAGQRPIRFALVVTAVLAAGALAQGVSGRLLHVERNFFGVVRVTYDANFNANRLFHGS
                     TLHGQQSLDPALRRQPSTYFTRSGPIGRMFRAMEQRLNQPGVRVAIVGLGAGTLASYA
                     QPGQRWTFYEIDAAMERIAKDPRFFTYLRDSEADAVDIVLGDARQRLGDAPDHAYQLI
                     VLDAFSSDAVPVHLLSREAIRLYRRKLANGGLLVFNLSSRYLDLDPLLGRQAADAELV
                     CRIAYDLNVTEDEKRAGKQPSIWAVLAGNESDLGILATDVRWQPPARRAHSSIWTDEY
                     SDLASYLILTPGPKWRRDRASVVGPRRG"
BASE COUNT          894 a         1175 c         1143 g          631 t
ORIGIN      
        1 ctggaacgac caaagggcac ggtcaagagg ggtaaacttc gatcccgttg gcttggtcaa
       61 gtcgctgggc gcatgcgccg cgcgatcgcg gctaggccaa ggcgccccca gcgagaccca
      121 gcgagtgagc acggagacct tatcgtcact aagcttgcca gtggggggca tctccagccc
      181 ggcgaagttg accgcctcga cgatgagact ttccgtaggc ttgcccggta caaccgcagg
      241 cccagattcg cccccgtgca ggatcgcctc gagcgaatcg agccgcaggc cacctttttg
      301 tttcttgtca ccatggcacg agtagcagtt ttccgccaaa aggggccgga cgctttgctc
      361 gaagaagtgc tcgcgggcag cctgggagtt cgagtcgggc tcgcctccac tagcgctcat
      421 cgagattgtc gcactcgcga ccgcaagtgc gattaccgcg atgttatagc gctgcgacca
      481 cggccgttga cgcaccctca ttggcagggg ccctcgcggt tcgcgttgga tgacaagact
      541 ggcactgagc tgtctcttgc catcggatcg tcggaatgga atgttcaata aagatagagc
      601 ataaagcgga ttggtcgacg attcaacgct ttgggattgg cggcgcgatg tattgtgcct
      661 tagaaggcga ttgctttcgc ctcaagccct cggcgatcac ccgctagaag tgggcgcgcg
      721 ggctgcgatt ggtgtttaag atcggctaac aggtagtgcc gaagtatcga agcttcacgc
      781 tcaaaaaaac gcaaaattgc agctccctgc tcaagtggta tcaggaagaa ttcgctagtg
      841 gggacgcgca acccggacag ggtcagacct cctggtgtac aattaatctg ttaaatcgac
      901 caccacggca aacgcccatg aatctggccg ttgtcgggcc ggaggcaaga aaggggggaa
      961 tccgatcatg aatcggccag agtcattgat ccggctgctt ccaacaagca tgcattggag
     1021 ccccgacggt gaggtgcgcg tggtgggtcg acggatcggg ctgttccata tcgtgaaagc
     1081 gcaccgcgtt tttggcgagt cgccggcgaa gatcgccgaa gaattcgagc tgccgctgga
     1141 actgaccgcc gaagttctgg cctttgctga aaaacacaaa gttgaagtgg atgcttatgt
     1201 ggccgactat caggccgagc tcgaccggca atgtgcggcc tatcaaccca gcccgacgcc
     1261 gctgcggatc agccgtcagg tcgcggaacg acgggcacag gaatcgcagc cggaatgctc
     1321 agcccgacca gtggcgcgat aaggtgatct acatcccctg agtcatcctc gacgcggccc
     1381 cacaaccgac gcacgatccc gtctccactt cggcccgggc gtcaagatca ggtacgacgc
     1441 cagatccgag tattcgtcgg tccagatcga cgaatgcgca cgccgggcag ggggctgcca
     1501 acgaacgtcg gtcgccagga tgcctaagtc cgactcattc ccggccaaca cggcccagat
     1561 cgagggttgc ttgccggctc gtttttcgtc ttcagtgacg ttcaagtcgt aggcgatccg
     1621 gcacaccagt tccgcgtccg cggcttgccg gcccaagagc gggtcgaggt cgaggtagcg
     1681 actcgacagg ttgaagacca gcaagccccc gttggcaagc ttcctgcggt agagcctgat
     1741 cgcctcgcga gagagcaaat gcacagggac tgcgtcggaa ctgaaggcat cgagcacgat
     1801 caactgatac gcgtgatcgg gagcatcgcc caggcgttgc cgcgcatcgc ccaggacgat
     1861 gtcgaccgcg tcagcctcag agtcgcgtag ataagtgaaa aaccgcggat ccttggcgat
     1921 gcgctccatc gcggcatcga tctcgtaaaa cgtccaacgc tgcccaggct gggcatagct
     1981 cgccagagta cccgcaccca gccccacaat cgcgacccgt acacctggct ggtttagccg
     2041 ctgttccatc gcgcggaaca tccgcccgat tggccctgac cgtgtaaaat aggtcgacgg
     2101 ttgccggcgc agggccggat ccaggctttg ctgtccatgc agcgtactgc cgtgaaacag
     2161 tcggtttgcg ttaaaattcg cgtcgtaagt cactcgaaca acaccaaaga agttgcgctc
     2221 gacgtgcagc aaccggccgc tgacaccctg tgccaaagca ccggccgcca gcacagcggt
     2281 aaccacgagc gcgaaccgga tcggcctttg ccctgccgtc atgcaggcga gaatccccag
     2341 gcccgaggca acaatcagcg ccaacgcgcc caacacagaa tcgcccagcc caccttggtt
     2401 cgtcgccaac gatgctgcta acacgaacac gaccccggcg aacacgaaat cccccagcca
     2461 ttcccgcggg ctgcgtcccc agtctcgtat cttggtcaaa ggcgcggcca ggcacgtcag
     2521 gacgatcgcg agcggatatt cgacgacccg gtcgaataag atcggcgcgg cgatcgcggt
     2581 ccagattcca cccaagagtc cccccagggc gatcgtcagg taatagagcg acagccgctc
     2641 cgtctccggt cgcatctgcg ccagcgcacc atggcatgca acggaagccg caaaaaacgc
     2701 gatcaagtgc aggggaatcc agtaaacgtg aacgaagccg gcgctcatga ccaacaccag
     2761 cggtgcgatc aaaaaaggga gcgacctggt ggctagccgg acgaatgcca cagccgaccg
     2821 cgcgaaggcc agaataaagc tcagcaagta gagcgcaagc ggtatgatcc aggcgagtgg
     2881 gaccgacgcc atatcggtcg tcaggtaggc cgtcacaccc aagagccagc tcgatggaat
     2941 gaaaaccaat gcgagccatc ggagccagat ggctaacgcg aagcgtcgct ccgcgccgtg
     3001 caagtcgaac agacgaggcg aattgccgac gggtcgagat cgattcaacc accgggccaa
     3061 gagaacacaa gccacgagaa gagccgccag aatgagaaag cccgtcttcc agagctggct
     3121 ttgagcggtc aaacccaggt tgggctcgat cacgaacggg tagatcaaaa gcgccagcag
     3181 actccccgta ttgctcgccg cgtagagaaa ataaggatcg tctgcgcgac gatgcccggt
     3241 cagtgcaaaa gagcgctgta ccaaaggcgc agtcgccgaa accagcacca gcggcaacgc
     3301 ggccgagcgc aacaaaactc ctagcaagac caaggccgga tcgccggccg acgaggatct
     3361 tcgatcgctg ttgggttcga gcacgatcgg ctgcatgtag taaccgagcg ccaatgaggc
     3421 aacgaatccc aagaggtaca cagcgcccgc acgtccgcgc aagctccagc ccgtgcggcc
     3481 cagcccgtac gtgaagagat acccgcacaa gagcgtcccc tgaaaaaaaa cgaggctcgt
     3541 attccagaca gcggaagtcc ccccaaagag cggcaacgcc attttgccga tcatcggctg
     3601 caccacgaac aggaggaacg cacctacgaa cgaggccagg gcaaaaagga ccacgattcc
     3661 ggattgcatg aattcccgac tcttgttttg ttgcgcgagc gcaagcattc acctcgcgtc
     3721 tggcataaac caacgagggc acgcgccagg ccgtttgtgt ggctggggag gcgaggttag
     3781 agcgaaccta ctacaataac aagtccgagc gcggcgtagg atgcccgcaa gttccttcgg
     3841 cct
//
LOCUS       DATAQL010000561         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4025854, whole genome shotgun sequence.
ACCESSION   DATAQL010000561 DATAQL010000000
VERSION     DATAQL010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4025854"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VHS97_09725"
     CDS             complement(<1..340)
                     /locus_tag="VHS97_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3448521.1"
                     /translation="MRRIALAVLMVIGLFVTGPTLAADPQVVELWPGKTPGDVGIKGM
                     ETSRIHRSPLVGPTKLITNVSKPTLTVYLPSKDKNTGTAMLICPGGGYWDLYWELEGE
                     EVAAWLNSVGM"
     gene            complement(470..1825)
                     /locus_tag="VHS97_09730"
     CDS             complement(470..1825)
                     /locus_tag="VHS97_09730"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX3448522.1"
                     /translation="MDQPGGARRVVIPWGPLESLELELPAAWPAADLVCPDLGGVLAN
                     YRDALSTALERPVGLDPLDRLARPGMTVAIIVDDPSRWTPVRDALPILLRFIHAAGVR
                     QEDVTISVGVGRHRPVDAPAMRARVGDEVANMYPCHSPPVDDLEQYVYLGQTAQGVPV
                     RVFRPVAAADLRILVGSVLPHLQAGFGGGYKLIFPGCSHRSTLVALHRQGLGGDAGGL
                     LGGDLATNPMRVAIRAAAALLPGPCLSISHLLGDQGQVLRVVAGHPDAAQDALSIEAR
                     SRFRAPPALPADLVVAGNAPWPGDPMMSFKVLLQHRAAGKPGGVLAGFFWTNPAEIDR
                     TVPMAATKAVAATGAVGGYVARRGLKLADRIAAAINSPASFMIRWARELVVDRTVLVY
                     APPLRERFGPRLGPVHLFADQASLWREAARAVPHDRNEPMRVRVFPHGGLTYACSAQS
                     T"
BASE COUNT          452 a          700 c          668 g          345 t
ORIGIN      
        1 tcatgccgac cgagttgagc catgccgcca cttcctcgcc ctcgagctcc cagtagaggt
       61 cccaatatcc gcctcccgga caaatcagca tcgccgtccc ggtgttcttg tcctttgaag
      121 gcagatacac agtcagggtc ggcttgctga cattcgtgat cagcttcgtt ggtccaacga
      181 gtggtgaccg atgaatccgg ctggtctcca tacccttgat cccgacatcg cccggcgtct
      241 ttcccggcca gagctcgacg acctgaggat ccgccgccaa agttggacca gtcacgaaca
      301 gcccgataac catcaggact gcgagagcga ttcgtcgcat cggaaaacac ctcgtgaagt
      361 ttgacggtga ttgctcgtaa actcgcgtga ttcattttgc caaactccta gtcttggaat
      421 ttgacccgtc atcgtatgac aagtgtcacc gtttgcgcaa gtgtggccat cacgtgctct
      481 gggcagagca cgcataggtc agtccaccgt ggggaaagac gcgaacccgc atgggctcgt
      541 tccgatcgtg agggacagcg cgggccgcct ctcgccacag ggatgcttgg tcggcgaaaa
      601 ggtggacggg acccagacgc gggccgaatc gctcgcgcag ggggggcgcg tagaccagga
      661 cggtccggtc cacgaccagc tcacgggccc aacggatcat aaacgaggcc gggctgttaa
      721 tggccgcggc gattcgatcg gcgagcttga gaccacggcg agcgacatag ccgccgaccg
      781 cgccggtcgc ggcgacggcc tttgtggcag ccatcggaac tgtacgatca atctccgccg
      841 gatttgtcca gaagaagccc gccaagacgc cccctggctt cccggcggcc cggtgctgaa
      901 gcaagacttt gaagctcatc atggggtcgc ccggccatgg cgcattgccg gcgacgacca
      961 ggtcagccgg cagggcagga ggtgcccgga accggctccg ggcctcgatc gagagagcat
     1021 cctgggcggc gtccggatgg ccagcgacaa cccggagaac ctggccctgg tcacccagca
     1081 agtggctgat ggacaagcag ggccccggca agagtgcggc cgccgcgcgg attgcgactc
     1141 gcatgggatt ggtcgcgagg tcacccccga gcaatccgcc cgcgtccccc ccgagacctt
     1201 ggcggtgcag cgccaccaag gtcgagcgat gactgcatcc cggaaaaatc aacttgtagc
     1261 cccctccgaa cccagcctgc aggtggggca acaccgagcc gacgagaatg cgaaggtccg
     1321 ctgccgccac cgggcggaag accctgacgg gcacgccctg agcggtttgg cccagataca
     1381 catactgttc caggtcgtcc accggcgggc tgtgacaggg atacatgttg gcgacctcgt
     1441 cgccgacgcg tgcgcgcatc gcaggggcgt caacgggacg atggcggccg accccgacgc
     1501 tgatcgtgac gtcctcttgc cgcacgccgg cggcatggat aaaccgcagg agaatcggca
     1561 gagcgtcgcg gaccggtgtc catcgtgacg gatcgtcgac gataatcgcg accgtcatac
     1621 cgggccgcgc gagccgatcc aacgggtcga gcccgacggg tctctccagg gctgtggaca
     1681 gagcgtctcg atagttcgcc agcacgccgc ccagatcggg gcaaaccaag tcggccgccg
     1741 gccatgccgc gggaagctcg agttcaaggc tctcgagcgg tccccacggg atgacaaccc
     1801 tccgtgctcc tccgggctgg tccaagcaat gatccatatt tgacggtacg agaacgcaac
     1861 cgacgccttt gcgatgatcc cccaataata cccgaatcca atggtcaaag gcacggccca
     1921 ggtcgcccag cgaaataaaa ccaatgggcg ctcaaggcgt caggcacctt aaacacgacc
     1981 agaccggtca ggaattagac gcaccgaagc gaacaggtcg acatatcggg tccggaccgc
     2041 tttgttcgca caaaaatctg gggcgggcgt ctaagaaacc agactgccgt acgtagtccc
     2101 atatgaaacg gcacacgatc cccttccgag cagggtgatg tcgagaacac cgaaccccga
     2161 gcgcg
//
LOCUS       DATAQL010000562         6005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_361122, whole genome shotgun sequence.
ACCESSION   DATAQL010000562 DATAQL010000000
VERSION     DATAQL010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361122"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VHS97_09735"
     CDS             <1..692
                     /locus_tag="VHS97_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245409.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEX3448523.1"
                     /translation="VTYEVLTRDGETLHISNPTRIPDAGAIEEFREPIARLNFIIPSD
                     SIGAIMQLCEDRRGVYLKTEYLSPSRAILTYELPLAEMIYDLYDKLKSATRGYGTMDY
                     EVIGYRAGDLCRLDILVAGQRVDALSSIVHRMHADRRGRKLVKKLKGEIERHQFEVAI
                     QAAIGSRVIARESISALRKNVTAKCYGGDITRKRKLLEKQKEGKKRMKQVGNVEISQE
                     AFLSVLDDSED"
     gene            717..2177
                     /gene="lepB"
                     /locus_tag="VHS97_09740"
     CDS             717..2177
                     /gene="lepB"
                     /locus_tag="VHS97_09740"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX3448524.1"
                     /translation="MTASTLMHECLVRPHEVPRAGLIRQTVEMIVLLCLCVVLVRTFS
                     AEAYVVPTGSMAPTLLGQHRELVCDSCRFVFVVGIEEEGHSGQAVCPNCGRHARDETP
                     SIECGGDRVLVQKFLYDFRRPKRWEVAVFHFPGEPTQAYVKRVVGLPGDSIRIAGGDI
                     YVDGQIVRKTLPEIRAMRVLIHDSRFQAQDAGRLPRWQFRAESRYGPLESGWTQRDGT
                     FAHAAVNPDARPHVDWLVYRHFDPSKGQYGPVRDFYGYNGGDLHVDNEVADVGMEARL
                     RVSDSVESISLSLRSGREHFLVRIPVGRPRSIELLQNNARKSLTNCRNPFEENNLGPR
                     SALLEAAVFDRRVQVSIDGQPLFDPFDFDASVPAGNSGESPIALGVRGGELEVSDFLI
                     YRDIYYTSSLANTPRHPHGMSTAVRLGADEYFVLGDNSPVSNDSRFWNEGPVVRGSMF
                     VGRPFLVHLPGQVVPLKVFGRSVCWVPDPRQIRYIR"
     gene            2310..4046
                     /gene="lepB"
                     /locus_tag="VHS97_09745"
     CDS             2310..4046
                     /gene="lepB"
                     /locus_tag="VHS97_09745"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX3448525.1"
                     /translation="MGRSSATLQAAPKPAKVEIKHHTKEGHRDTVEAIVVAFILALVV
                     RGFEAQAFVIPTGSMAPTLMGRHKEIACPQCGFTFAVNASEEVEQHSPSHSVYSGLCG
                     NCRHQAVRLDDEPSFKGDRILVMMFPYDLPFLPGSGPPARWDVVVFRYPEDPEVSYIK
                     RLVGLPGDTVRIYHGDVYVKSAGSSAFALARKTLRHQSAMQINVYDDRYRPKALLGKA
                     EWQRWQGDSPIWKVVDRAISRYESEGSPKDEWSVLRYRHLVPDPEQWEAIQDDRALPR
                     EARSTLITDFYSYNTNMSEKYHNLLDDVQRDQENAWMQPHWVGDLTLEANLELSKIDT
                     NASVRLELVKAGLPHRCTIELATGTAIVTRGEDELARWETPIKGPGRYKIEFANVDDR
                     VSLVVDGRPVGGEGIEFESQQGAPISTAADLAPAAIAVRNAALVTTDLVLKRDIYYTQ
                     SPGRIDYGIVWEDRYPRTPVELFDFLADPSRFPSLANVRSREYEIGPDRYFMMGDNSP
                     RSKDSRGWGTDDSAWDSSERKSHEVPRQLLTGKAFFVYWPHGVPVWPGIALGPDTRVP
                     FRPYVERMKRIR"
     gene            4080..5039
                     /gene="lptB"
                     /locus_tag="VHS97_09750"
     CDS             4080..5039
                     /gene="lptB"
                     /locus_tag="VHS97_09750"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX3448526.1"
                     /translation="MPLLQVRGLEKWYGRRQVVNGVDYEVERGEVVGLLGPNGAGKTT
                     SFRMTIGLIDADGGNVMFDGRDVTRLPMYLRARAGMGYLAQDSSVFRQLSVEDNLMAI
                     LETRSQLTRKQRKDRQNDLLDQFGLTTIRKTKANRVSGGERRRLEIARSLITEPKMIM
                     LDEPFAGIDPKTVIEIQDQIRALVEQYNIGILLTDHQFRETLEVTDRCYLIREGRVFA
                     YGNREQILRNPDVRRHYIGERFDVGHLLDQRRPVAIPHSAPADSGETIPDDGPNDPSS
                     IFVDQYVTTEMISGQIDDLSVQSIQTQMGCSDSENGDDTETRH"
     gene            5137..5202
                     /locus_tag="VHS97_09755"
                     /pseudo
     CDS             5137..5202
                     /locus_tag="VHS97_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5228..>6005)
                     /locus_tag="VHS97_09760"
     CDS             complement(5228..>6005)
                     /locus_tag="VHS97_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX3448527.1"
                     /translation="TIDLQGLLRSALMTAATRAQVRLGLADAREGARWFYTDVVDAPR
                     LGLHAVERVQRVAAALHLDATQPRFNLPIRETDRQWAREVLAAVRSPRIVLNVGARWE
                     TKRWPPEHFAEIGRRAAHEFGAGLIAVGSDGDRPLVDQLIRHLGAIPVLYLCGQTRLM
                     QLAALCTESDLVISNDTGPLHLAAAAGARVVGIYTCTSPGLTGPFGPRVATVQSCVWC
                     APSFIKKCHRLECMAELTPNRVWPIVKAQFEPPIMVGQVG"
BASE COUNT         1227 a         1735 c         1823 g         1220 t
ORIGIN      
        1 atgtgaccta cgaggtcctg acccgcgatg gtgaaaccct ccatatctcc aacccgacgc
       61 gtataccgga cgccggagcg atcgaggagt ttcgcgaacc catcgcccgc ctgaacttca
      121 tcattcccag cgattcgatc ggcgcgatca tgcagctttg tgaagaccgt cgcggggttt
      181 acttaaagac tgagtatctc tcgccgtcgc gtgcgatcct cacttacgaa ctgccgcttg
      241 ccgagatgat ctatgatctt tatgacaagc tgaaaagcgc gacacgaggt tacggtacca
      301 tggattacga ggtgatcggc tatcgcgccg gcgatctttg ccggctggac attctggtcg
      361 ccggtcagcg cgtcgacgcg ctctcatcga tcgtccaccg catgcatgcc gatcgccgtg
      421 gtcgcaagct ggtcaaaaag ctcaagggcg agatcgagcg tcatcagttc gaggtggcga
      481 ttcaggcggc gatcggcagt cgagtcattg cccgcgaaag catttccgcc ctgcggaaaa
      541 atgtgacggc caaatgttat gggggtgaca tcactcgaaa aaggaagctt ctcgaaaagc
      601 aaaaagaggg caagaagcgt atgaaacagg tagggaacgt cgaaatttcg caggaagcgt
      661 ttctatctgt tctcgacgac agcgaagatt gatcctaaaa gactgatcgg acgacgatga
      721 ccgcctctac attgatgcat gaatgcctcg tccgccccca cgaagtgccc agggccgggc
      781 tcattcgtca gacggtcgag atgatcgttt tgctctgcct ctgcgttgtc ttggtgcgga
      841 cattttcggc tgaggcgtac gtcgtcccga cggggtcgat ggctcccacg cttttgggac
      901 agcaccgcga actcgtttgc gacagttgcc ggtttgtgtt cgtcgtgggg attgaggaag
      961 aaggacatag tggccaggcg gtctgcccga actgcggcag gcacgctcgc gacgaaactc
     1021 cgtcgatcga atgtggcggc gacagggtct tggtgcagaa gttcctttat gatttccggc
     1081 ggcccaaaag atgggaggtc gccgtttttc atttcccggg cgaacccact caggcctatg
     1141 tcaagcgcgt ggtggggttg cccggcgatt cgatccggat cgccggaggt gacatctatg
     1201 tcgatggaca gatcgtcagg aagacgttgc cggagattcg ggcaatgcga gtcctcatcc
     1261 acgacagcag gtttcaagct caagatgccg gccgacttcc gcgctggcaa tttcgggccg
     1321 aatcgcgtta tggcccgctt gaaagtggtt ggacccagcg agatggtacg ttcgcacacg
     1381 ccgccgtcaa tcccgacgcg cggccgcacg tcgactggct cgtctataga cacttcgacc
     1441 cctcaaaagg ccagtatggg ccggtccgtg atttttatgg atataacggc ggcgatctcc
     1501 acgtcgacaa cgaggtcgcc gacgtgggga tggaagcacg cctgcgcgtc agcgactcgg
     1561 tcgagtcaat ctcgttgagc ctccgatccg gtagggagca tttcctcgtg aggattcccg
     1621 tgggcaggcc gcgctcgatc gagctgctac aaaacaatgc gcggaaatca ctgacaaatt
     1681 gccggaatcc ttttgaagaa aataatttag ggccgagatc ggcgctgctg gaggcggcgg
     1741 tctttgatcg tcgcgttcag gtttcaatcg acgggcaacc gttgttcgat ccgtttgatt
     1801 tcgacgcctc ggttcctgcc ggaaattcgg gcgagagtcc gattgcattg ggggtacgcg
     1861 gtggggagct cgaggtcagc gacttcttga tctatcgcga catctactac acctcatcgc
     1921 tcgccaacac accgcgccat ccgcacggaa tgtcgacggc ggtgcggctc ggtgcggacg
     1981 aatacttcgt gctgggcgat aacagcccgg tttccaatga ttcgcgattc tggaacgagg
     2041 ggccggttgt gcgcgggtcg atgttcgtgg ggagaccgtt tttggtccat ctgcctggtc
     2101 aggtagttcc gctcaaggta tttgggcgat ccgtgtgctg ggttcccgat cctcgccaaa
     2161 tccgttacat tcggtagcga ggtaattccg ccataaggtg ggtgaggctt ttccccagtc
     2221 gagcggccgg gcgactcgct ttttatgatc cagacgacat gccattccct gagttcccgg
     2281 gtgagctcaa cgagaggaaa catctgacga tgggacggtc ctcagccact ttgcaagccg
     2341 cgcctaagcc cgccaaggtc gaaatcaagc atcacaccaa ggagggccac cgcgacacgg
     2401 tcgaggcgat cgtcgtcgca ttcatactgg ccttggtagt gcggggtttc gaggctcagg
     2461 ctttcgtgat cccaacgggt tcgatggctc cgacactgat gggccggcac aaggagatcg
     2521 cctgtcctca atgtggattc acttttgcgg tgaacgcctc ggaagaagtc gagcaacact
     2581 cgccgtctca cagtgtttac tcagggctgt gcggtaattg ccgtcatcag gctgtgcggt
     2641 tggacgacga gccgagcttc aagggcgatc gaattcttgt gatgatgttt ccatacgacc
     2701 tgccattcct gccggggagc ggtccccctg cgcgctggga tgtcgtcgtg ttccgctatc
     2761 ccgaggaccc ggaagtcagc tatatcaagc gactggtggg actcccaggc gatacggtcc
     2821 ggatctatca cggcgatgtc tacgtcaaaa gcgcggggag cagcgcattt gcgttggccc
     2881 gcaagacgtt gcgacatcaa tcggcaatgc agatcaatgt ctacgatgac cgttaccggc
     2941 cgaaggccct attgggcaag gcagagtggc agcgatggca aggcgattca ccgatctgga
     3001 aggtcgtcga tagagcgatc agccgatacg agtcggaggg aagcccgaaa gacgagtggt
     3061 ccgtgttgcg gtaccgtcac ctggtacccg atcccgagca atgggaggcc atccaggacg
     3121 atcgagccct gccgcgcgag gcccgctcga cgctcatcac cgacttttac tcgtacaaca
     3181 ccaacatgtc ggagaagtac cacaacttgc tcgacgatgt ccaacgcgat caagaaaacg
     3241 catggatgca gccccattgg gtcggcgatt tgactttaga ggcgaatctc gagctgtcca
     3301 agatcgacac gaacgcgtcg gttcgccttg agctcgtcaa ggctgggctg ccgcaccgct
     3361 gcacgatcga gctcgcgacc ggtacggcca ttgtgactcg tggcgaggat gagctggcta
     3421 ggtgggagac gccgatcaag ggccctgggc gctataagat tgagttcgcc aatgttgacg
     3481 atcgtgtcag cctcgtggtc gatggacgac cggttggcgg ggaaggcatc gagtttgaat
     3541 cgcagcaagg ggcgccgata tccacggcgg ccgacctggc gccggcagcc atcgcggtcc
     3601 gaaatgcggc tctcgtcacg accgatcttg tcctgaagcg cgatatctac tatacccagt
     3661 caccgggccg aattgattat ggaatcgtgt gggaggatcg ttatccccgg acgcctgtcg
     3721 aacttttcga cttcctcgca gacccttccc gatttcccag cctggccaat gtgagatcgc
     3781 gagagtacga gatcggtcct gaccgatact tcatgatggg cgataatagc cctcgcagca
     3841 aggatagccg cgggtggggg accgatgatt cggcatggga ctcgagcgag cgcaaatcac
     3901 acgaggttcc acgccagctc ttgaccggca aagcgttctt tgtttactgg ccgcacggtg
     3961 ttccggtttg gccgggaatt gcacttggcc ccgatacacg tgtgccgttc cgtccgtatg
     4021 tcgaacgcat gaagcggatc cgctgaccct gactaaatcc gatcaaggag gatcgagcga
     4081 tgcccctctt gcaggtccgc gggctggaaa agtggtatgg ccgccgccaa gtggttaatg
     4141 gcgtcgacta cgaagtcgag cgtggcgagg tcgtcggatt gctggggccc aatggtgccg
     4201 gcaagacgac cagcttccgg atgacgatcg gactgatcga cgccgacggc ggcaatgtca
     4261 tgttcgacgg tcgcgatgtg acgcgcttgc cgatgtacct gcgcgcccgc gccggcatgg
     4321 gttacctggc gcaggattcg agcgtgttcc ggcagttatc ggtcgaggac aatctcatgg
     4381 cgatcctcga gactcgctct caactcacac gcaagcagag gaaggaccgg caaaacgatt
     4441 tgctcgacca gttcggcctg acgacgatcc gcaagaccaa agccaatcgc gtttcgggtg
     4501 gcgagcgccg gcgtctcgag attgcacggt cgctgatcac cgagcccaag atgatcatgc
     4561 tcgatgaacc gttcgccggc attgatccca agacggtgat cgagatccag gaccagattc
     4621 gcgcgcttgt cgagcagtac aacatcggga tcctcttgac cgaccaccag ttccgcgaga
     4681 ccctcgaggt caccgatcgc tgctacttga ttcgcgaagg gcgggtgttt gcgtatggca
     4741 accgcgagca gattctccgc aaccccgatg tccgccggca ctatatcggc gagcgcttcg
     4801 atgtcggcca cttgctcgat cagcgccgac cggtagcgat accccactcc gcccccgcag
     4861 actccggcga gacaatcccg gatgacggcc cgaacgaccc gtcgtcgatt ttcgtggacc
     4921 agtacgtcac gaccgagatg atctcgggcc agattgacga tctgtcggtc cagtcaatac
     4981 agactcagat gggctgttcc gattcggaaa acggggatga tactgaaacg aggcactgat
     5041 ttgccgagga ctcagtgcct tatcaccctt ctagttgccg gttcggtgtt taaccagcag
     5101 tccgaattta gcagggcgtt gcgaagcacg tgattcccgc ctgagttcac tctttctgcc
     5161 gctatgaacc cctgcccatg cggctatcct tcgcagcggt agccggcgca gtgtgagatg
     5221 ccggtcctca acccacttgt cccaccatga tcggcggctc gaactgtgcc tttacgatcg
     5281 gccacacgcg gtttggagtc agctcggcca tgcactccaa tcgatggcac tttttgatga
     5341 agctgggtgc gcaccagaca cagctctgaa cggtggcgac gcgcggtcca aaaggacctg
     5401 taagtccagg gctcgtgcat gtatagatcc cgaccacgcg cgcgccggca gccgccgcca
     5461 gatggagcgg gccggtgtcg ttggagatca ccaggtccga ctctgtgcac aaggccgcga
     5521 gctgcatgag ccgggtttga ccgcaaagat agagcacagg aatagccccg agatgtcgaa
     5581 tgagctgatc gacgagcggt cgatcgccgt cagatccgac ggcaatcagg cccgcgccga
     5641 attcatgggc agcgcgacgg ccgatttccg cgaaatgctc gggcggccag cgcttggtct
     5701 cccagcgcgc tcccacattg agcacaatcc tgggcgatcg taccgccgcc aggacctctc
     5761 gggcccactg acgatcagtt tctcggatcg gcaggttaaa acgcggttgg gttgcgtcca
     5821 ggtgtaacgc ggccgccact cgctggacac gctccacagc gtgcaggccc agtcgagggg
     5881 catcgaccac gtccgtataa aaccagcgcg caccttcacg agcatcagcc agccccaagc
     5941 gcacttgggc cctcgtcgcc gccgtcatga gagccgaacg gagcagaccc tgtaaatcga
     6001 tagtc
//
LOCUS       DATAQL010000563         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4636375, whole genome shotgun sequence.
ACCESSION   DATAQL010000563 DATAQL010000000
VERSION     DATAQL010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4636375"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VHS97_09765"
     CDS             <1..289
                     /locus_tag="VHS97_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX3448528.1"
                     /translation="AAIVRAHRALGLPGAIVLAQPVPADHALDPDLLQAALDEALRQA
                     AVRRITGKPLTPFLLDSIRRTTHEQSVRANCALLESNARLAAEVAVLLANK"
     gene            complement(403..2088)
                     /locus_tag="VHS97_09770"
     CDS             complement(403..2088)
                     /locus_tag="VHS97_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448529.1"
                     /translation="MSVLALLGPWPFAPLAKAQDTPAKRDAAVQPEVPQELAPADRVQ
                     DSVPASAEALSERYRFIEKYSVNPEPDRPELLSQYRIGILETQKTEREKPQGAPERFQ
                     FSHVTTYTERPAQVGKLGEVISAVRRYDTFRIKEMAATQPSKKRPFEGLTILYQRRTG
                     KEPLFLSLSGDHPLREFECSAMNKQIFVPQLTTLFAPTPQRVGDTWRISRQAMQCLVN
                     EVPDTEDYEMNGRLIEVRKTGTGTSLTAVIGISGQMNLSHGKSVLNAQIHFVFEPTPA
                     VAPPLDAGASTKPAESAAVKARNKRDAGIINARGRISRVLMAWVASTPLEDDDGRLKQ
                     TDTYELDLERRVAPVTSDATGTQNTLLTIPDPLPSADESNSWVLYEDPHGRFHFRHPQ
                     ELRLEPRLADPDNIGLVDQQLRGSDSLAISLVPKEASPERARQNRDPDFHRRNLYSMW
                     EKQKRDMVRGSTGWLPDAEWTPLKRKVYRIEAALRQSGPDAGTAPRIYCDYYLVLFNT
                     NQSIVVTAMTVQDPHIGYRNQAEAVIKSFQFGTADAATKPAVPPSAPPETPPG"
BASE COUNT          483 a          751 c          731 g          554 t
ORIGIN      
        1 cgcggcgatc gttcgcgccc atcgcgcgct cgggctccct ggcgcgatcg ttctggccca
       61 gccggttccg gccgaccacg ccctcgatcc cgacttgctc caagcggccc tggacgaggc
      121 cctccgacaa gccgcggtgc ggagaatcac cggaaagccg ctcacacctt tcttgctgga
      181 ctcgattcgg cggaccaccc atgaacagag cgtgcgtgcc aattgcgctc tcttggagtc
      241 caacgctcgt ctcgcggcgg aggtcgccgt tctgcttgcc aataaatgaa gcacgaaaag
      301 cgcgcgtacc gcacgaaagt aggaaccctt aatcgattag aggaggatac acatagctgg
      361 aaccgcaagc ggctcgtgca ggccaaatgc gtcgcttgcc aatcatcctg gtggcgtctc
      421 tggaggagcc gaaggtggta ccgcaggttt cgtcgccgcg tcggcggtcc caaattggaa
      481 actcttgatc acggcctcag cctggttgcg atagccgata tgcggatcct gcacggtcat
      541 tgccgttacc acgatgcttt ggttcgtgtt gaacaggact agatagtagt cgcaatagat
      601 tcgcggcgcg gtgccggcgt ccggcccgga ctgtcgcaga gcggcttcaa tgcgatacac
      661 cttacgtttt agcggggtcc actccgcatc cggaagccat ccagtcgagc cgcgaaccat
      721 atcgcgtttc tgtttctccc acatcgagta aagatttcgg cgatgaaaat ccggatcacg
      781 attctggcga gcacgttccg ggcttgcctc ctttggaacg aggctaatag caagggaatc
      841 agaaccgcgc agctgctggt caaccaaccc gatgttgtcg gggtccgcta atcgaggctc
      901 gagacggagc tcttggggat ggcggaaatg gaaacgtccg tgaggatctt cgtagagcac
      961 ccaggaattc gattcgtcgg cggacggaag agggtcgggg attgtgagga gcgtgttttg
     1021 agttccggtg gcatcgctcg tcacgggagc gacccgccgc tcaaggtcca gctcataggt
     1081 gtcggtctgc tttaggcggc cgtcgtcatc ctcgagcggc gtcgatgcaa cccacgccat
     1141 caacacgcga gaaatcctgc ctcgagcatt gatgattcct gcatctcgct tgttacgggc
     1201 cttgacggca gcgctctcgg ccggttttgt gctggccccc gcgtctaatg gcggcgcaac
     1261 ggccggtgtc ggttcaaata caaaatggat ctgtgcgttg agcacgctct tgccgtgaga
     1321 caaattcatc tgaccggaaa tgccgatgac agcggtgaga ctggtccctg tcccagtctt
     1381 gcgcacctcg atcaggcgac cattcatttc ataatcctcg gtgtcgggaa cctcgttcac
     1441 cagacactgc atagcctgac gtgaaatccg ccacgtgtcg cccacgcgct gcggtgttgg
     1501 tgcaaaaagg gtcgtcaatt gaggtacaaa gatctgcttg ttcatcgccg agcattcaaa
     1561 ctcacggagg gggtgatcac ccgaaaggct caagaataga ggttctttgc ctgttcgtcg
     1621 ctggtacaag atcgtcaagc cctcgaacgg gcgcttcttc gaaggttgcg tcgctgccat
     1681 ctccttgatg cggaacgtgt catagcggcg aactgcactg atcacttcgc cgagcttacc
     1741 tacctgagcg ggccgttccg tatacgttgt cacatgggaa aactggaatc gttcgggcgc
     1801 gccctggggc ttttcgcgtt ccgttttctg ggtctcgagt atcccgattc ggtattgaga
     1861 aagcagctcg ggtcggtcgg gctcggggtt gacgctgtac ttttcgataa accgataccg
     1921 ctcgctgaga gcctcggcgg aggccggtac cgaatcctga accctgtcgg ccggcgccag
     1981 ttcctgtggc acctcgggtt ggaccgcggc atctcttttg gccggagtat cctgagcttt
     2041 ggctaatggc gcgaatggcc acggtccgag caaggccagg acgctcatta atccgacaac
     2101 cgtgctcccg ccccgatacc gtcttgagcg aatccctgtg cagcagaccg ctcgccctct
     2161 cttttcgtcg tccaccgtag cggccaaacg gtttcgctct tcacccatgg tgcaccgtct
     2221 tcggatctta gtgtttgttg gcattcccca accagatcgc ttgaccacga tcgggtggcc
     2281 ctgacgaatc gatcccgcgc tttctattag atcataatat aggctacatc cggtacggta
     2341 gagatcgcgg aagacccgcg gttccgcgag gtgatctcat tcatccgcat cgtcgaggcg
     2401 cagacatcct tatcccgctc cgcgcacgga tcatttctcc gatgagacga tcaatcgcgc
     2461 gattccggct gcgaccgcct gagccactgg agcgagccta tcatcaagag gtcctgttc
//
LOCUS       DATAQL010000564         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2194963, whole genome shotgun sequence.
ACCESSION   DATAQL010000564 DATAQL010000000
VERSION     DATAQL010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194963"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VHS97_09775"
     CDS             complement(<1..1217)
                     /locus_tag="VHS97_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3448530.1"
                     /translation="MTNYNHSKFLVLALVAAGTTSALAFETALADDKPSSTVLTGRDA
                     LGDWTTDAPGVRRMITVNDLAKPFETPSANNFPRPVRRPEGAMPRAPKGFRVSEFATG
                     LNNPRKIVTAPNGDILLAESMPGRIKLLRDSDGDGKAETSENFATGLSRPFGIAFYPP
                     GPTPTHVYIGNTDSVVRFPYQVGDTKPRGPAEVIVKSIPSGREQVGGGGHWTRDVEFS
                     RDGKILFVSVGSRSNNDDDQREKRRADILAFDPDGQNERIYASGIRNAVGLAIHPQTG
                     ELWASVNERDALGDHLVPDYVSHIVEGGFYGWPWFYIGPHQDPRYQGKHPELKDKTIV
                     PDVLVQSHSASLCMTFYTGLQFPPEYRLDAFAAEHGSWNRARRTGYKVIRIPMKDGKA
                     TGEYEDFLVGFVTP"
BASE COUNT          299 a          441 c          498 g          334 t
ORIGIN      
        1 ggagtgacga agccgaccag aaagtcttcg tattcgccgg tggccttgcc gtctttcatc
       61 gggatgcgga tgaccttgta acccgtacgc ctggcgcgat tccacgagcc gtgttcggcg
      121 gcaaaggcgt ccaagcgata ttccggcgga aactggagac cggtgtagaa ggtcatgcac
      181 aacgatgccg aatgggattg aacgagcacg tcgggaacga tggtcttgtc cttgagctca
      241 gggtgcttgc cttggtagcg cgggtcttga tgtggaccga tgtaaaacca aggccatcca
      301 taaaagccac cttccacaat gtggctgacg tagtcgggca ccaagtgatc gcccagtgcg
      361 tcgcgctcgt tcaccgaggc ccaaagctcg ccggtctgcg ggtggattgc caggccaacg
      421 gcgttgcgga ttcccgaggc ataaattcgc tcattctggc cgtcgggatc gaaggcgagg
      481 atgtcagccc gtcgtttttc cctctgatcg tcatcgttgt tcgaccgcga gccaaccgac
      541 acgaagagga tttttccgtc gcgtgagaat tccacatcgc gagtccagtg gccgccgcca
      601 ccgacctgct cgcggccgct ggggatgctc ttgacgatga cctccgccgg gccccgcggc
      661 ttggtgtcgc cgacttgata ggggaaacgg accacggaat cggtgttgcc gatgtacacg
      721 tgcgtcggcg tgggaccagg gggatagaag gcgattccga acggccggct caatcccgtc
      781 gcaaagtttt cgcttgtctc ggctttgcca tcgccatcag aatcgcgcaa aagtttgatg
      841 cggcccggca tgctctcggc tagcagaatg tcgccgttgg gggcggtgac gatcttgcga
      901 gggttattga gaccggtggc aaattcggag acgcgaaaac ccttgggcgc gcgaggcatc
      961 gccccctcgg gccgcctgac gggtctcggg aagttgtttg ccgaaggagt ttcaaagggc
     1021 ttggccagat cgttgacggt gatcatgcgc cgaacgcctg gagcgtcggt tgtccagtca
     1081 cccagggcat cgcgtcccgt caacaccgtc gagcttggct tgtcgtcggc gagagcagtc
     1141 tcgaacgcca gcgcgctcgt agtgccagcg gccaccaggg cgaggacgag aaacttggaa
     1201 tgattgtagt tggtcatgag ttctttccca gttgtcgcag caatcgagga gggaatcggt
     1261 ccgttctggt cgcgatgagc ttggatcgaa gcaggctgaa acagcttgga ggatcattgt
     1321 attggcccac ggaatccggc tggccgggcc ccgggtcgtt ggcaggtcgg tattagatta
     1381 atcacgatcg gcgctgcatg ccacatccca ccgcaaccta gtgtcgtctc cagggatgat
     1441 ggtttgtatc ttcgaacgcc ccgtttcagt tgccgtcaaa gagcaccggc gacgccacgg
     1501 cgtagggctt gcccttgctg atcgtgattg aaaacgtacc tccgccattt cctccctggt
     1561 tgagtacatt ac
//
LOCUS       DATAQL010000565         5357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1583032, whole genome shotgun sequence.
ACCESSION   DATAQL010000565 DATAQL010000000
VERSION     DATAQL010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583032"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1212)
                     /locus_tag="VHS97_09780"
     CDS             complement(28..1212)
                     /locus_tag="VHS97_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX3448531.1"
                     /translation="MPHQFRVDRVRGCLLGMAVGDALGAPLEGLSGQQIRTHYGRVRN
                     YVDGVQAWKRKPYRWRMHGLYSDDTQQALALCDILLDYQRVDPERLADLYLALMTPKG
                     LFVGAHRGIGRSFRQVLAGLERGVPAHLTGQPTAGIGAAMRIAPIPLYFGDEVGPMFD
                     SAMAASLMTHRDIRSLSGALAVVHTIRRLVAGEERDPSLLLWVAADVVKDEKRIATTH
                     SDLVIKTDAYGRSLSRAIAHAESVLDAPREHALSALVDEANRHGANPMCKRPTMGFPP
                     ACIPACLYLLLSTDSFEEAVTEVVNLGGDTDTAGAILGAMAGAYYGADAIPKRWLDGL
                     QNRDGIEARAIALANRSAKGLNIPDLVATEHLLSRKESESFESVASFARGGGGRDANP
                     VL"
     gene            complement(2373..2999)
                     /locus_tag="VHS97_09785"
     CDS             complement(2373..2999)
                     /locus_tag="VHS97_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HEX3448532.1"
                     /translation="MINCQHALWRQGAMAGKEMQGMGVSSGRSRVADLAFRVFNRAVH
                     PDWFSTREFRRVEQLGWEADIRILEGGHAFVFRSGSIRLTEILAGPETVLPETGQLFH
                     SHVRRERSTILRPGGTIEYQCCLEVERVEIEIFRHLSEEIRLEASRNRLFHGFKSSNR
                     LAPEPISHISIGARVNDLAVQSFHTFPDECAIVRTQSLFELKVPASQR"
     gene            3248..3970
                     /locus_tag="VHS97_09790"
     CDS             3248..3970
                     /locus_tag="VHS97_09790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3448533.1"
                     /translation="MDLIVRTDPPLPLRAWKALRSRGAGYAWHKFLRRSLSDWPAWKR
                     RILYADPHRYWTLRGGDDYFREQEGQKARTLRARWLAERLARYRPTSILEVGCGYGKV
                     LGELRRQLDIPLVGVDFSRTQLEQACRFLANDRDIELLLSRGERLPFADHSFDMVVTS
                     AVILHNPPAIAERIRREVMRVTRRFAAHNEETNLSYNRFGYDTALWYRRQGIILAESG
                     PIPVDPDPSASQFCVAVIYPGE"
     gene            4014..>5357
                     /locus_tag="VHS97_09795"
     CDS             4014..>5357
                     /locus_tag="VHS97_09795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3448534.1"
                     /translation="MIDLGFAALLALIAAGFGKRMLDGMGQSPEHPLDAMALALPLGL
                     GLTALATLALGELGWLNFVGLSVLLAVLVELGIIAWFHLFREVRAWFRARVRTRSRTS
                     LSLFFAFCLGLVIVGTALTALTPVTDGDALCYHLQVPKVFLMRGSVGFSPDLHETVYP
                     LVTEMLYAVALAFRGPVACRGIQWVLGLVFAANVTALARPSLGRRAWWAGAIAISVPA
                     VSNGMSAPLNDVALAAFGTAALFAWVRLHERPTVSAAIVAGLFMGLAIGVKYPALVLC
                     GLLAPTIALRTIDGRATNADGTRPRWFTLAAIFLTTTAAVGGWWYLRAYIHTGNPVYP
                     FFRDVFGGAGLDEVLDPIKRPLTVTPFNLITALVPLSLHPERFDSFSHQFGPMFLLFL
                     PAVFLFRAPRRVLVLVGLAYAFLLVCLTQRQSMRFLLIALGPMSIGVAYLAGIWCK"
BASE COUNT          981 a         1688 c         1594 g         1094 t
ORIGIN      
        1 ggatactggc agtttagcct attctactca caggacggga tttgcatccc ggccgccacc
       61 accgcgcgca aacgaggcaa ccgattcgaa gctctcgctc tccttgcgac tgagcaggtg
      121 ttccgtcgcg actaggtcgg ggatgttgag gcctttggcg gatcggttgg ccagcgcgat
      181 ggcacgggcc tcgattccgt ctcggttttg cagaccgtca agccagcgct tcgggatcgc
      241 gtcggctccg tagtaggctc ccgccatcgc gcccaggata gccccggccg tgtcggtgtc
      301 gccgcccaag ttaacgacct cggtcacggc ttcctcgaag ctatcggtcg agagcagcaa
      361 gtatagacaa gcggggatac aggcgggcgg aaagcccatt gtcggtcgct tgcacatcgg
      421 gttggcaccg tgccgattgg cttcatcgac caatgccgac agggcgtgct cacgtggggc
      481 gtcgagcacc gactcggcat gggcaatggc ccgagaaaga ctgcgaccgt aggcgtcggt
      541 cttgatcact aggtcgctgt gggtggtcgc gatccgcttc tcgtccttga cgacatccgc
      601 ggcaacccag agaagcaggc tgggatcgcg ctcttcgccc gcgaccagac ggcggatcgt
      661 gtgcacgaca gcaagcgccc cgctcaggct gcggatgtcg cggtgcgtca tcaggctggc
      721 cgccatggcc gaatcaaaca tcggcccgac ttcgtcgccg aagtagagcg ggatcggcgc
      781 gatcctcatg gccgctccga tccctgccgt cggctggccg gtcaggtggg cggggacgcc
      841 acgctcgagc ccagcgagga cctggcgaaa actcctgccg atgccgcggt gtgccccaac
      901 gaacaatcct ttgggcgtca tcagcgccag atagagatcc gcgagccgct caggatccac
      961 tcgctggtaa tcgagcagaa tgtcacataa ggccagtgcc tgttgcgtgt cgtcggaata
     1021 caggccgtgc atccgccagc ggtacggctt gcgcttccag gcctgcacgc cgtccacata
     1081 gttccgcacg cgtccgtagt gcgtcctgat ctgctgaccg gatagcccct ccagcggggc
     1141 gcccagagcg tccccgacgg ccattcccag caagcacccc cgaacacggt caactcgaaa
     1201 ctgatgaggc atcgtggcag tgctcctcgg atgccgactc gctcacctgc cgtagcaggg
     1261 gcgattccat tcgatcagac gattcgcaag ataaccgcct ggccgaacca cgccaaccga
     1321 aatttaccct tacacgagag aaccgatcga tcgcgacgga gtgatggcac cgcgtgcgct
     1381 cgatgctgcc ttcacttagg atcgcctacg accctgaaca tttttttggc taattcttca
     1441 ttttgtcgag ctccacgtgt tcgactcgag cgcaagttgc cgcgctcaat tgcctttgat
     1501 gaccatttga tcaggaattt ccgtctgtgc ttttgaacgc agcccgatca gtctgccgcg
     1561 atcaggaagg cacttcgcgc cgatttttcc ggggtggggt atgcgaccag cctgactgac
     1621 ccaatatcga cgtgccgggg ccatcacacc caccttcaag aggaggtttc tagccgccct
     1681 aacggcagac ttcgtgcaat cggggggacc gttgccacgc tcgaaatcgc gacggcaatt
     1741 atccgccaac gaaacgatct tgtcacaccc agattgcggc ctcaaatcgg ttgacccggg
     1801 gagcgcacgg aaccgggaat agtcctcggt ctcccaaatc accacaagaa tatgcatcta
     1861 cctcagccgc cagccgcacg agtctcatct cacgtaaaca ttctcgtccc ggagtaggca
     1921 aaaaacgatc gagccacgga atcgccagga cgctttagcg tggcgacgcc gggttcggtc
     1981 ttgcttgagt gaaacaaatg aaggatcgga cgaacggccc gcgaatctcg ctccatcgct
     2041 cgtgaatcgg tgggcccgac cggctgcgga aagaaccccc tgtatgtaat aattcggggc
     2101 caaaatcatc ggccccgtac actctcgcgc cgctcgtagg gcccgccccg gaatactcca
     2161 aaaccgcgga cgaccttctg agaagcggaa caatgacttc gccgcaattt acaagtccgc
     2221 atccgccacg tcaactcgcc agagtgttta tatggcgccg acgtgattca tggcaatggg
     2281 acatgatgcc cgtcggcgct gattcaagcc cgctgggcta gccgcccgag cgataatctc
     2341 cgatccccgc gtctcgcccg acatttccgg gtttaacgtt gcgacgcagg caccttgagc
     2401 tcgaacagcg actgtgtgcg gacgatcgca cactcgtcgg gaaaggtgtg gaatgactgg
     2461 acagccaggt cgttgacccg cgcgccgatc gagatatggc tgatgggctc gggcgccagg
     2521 cgattggagg atttgaagcc gtgaaacagg cggttacgcg atgcttcaag ccgaatttcc
     2581 tcgctcaggt ggcgaaagat ctctatttcg acgcgttcga cctcgaggca acactgatac
     2641 tcgatcgttc cgcccggtcg gaggatggtg gatcgctcgc ggcggacatg ggaatgaaac
     2701 agttggccgg tctcgggaag cacggtctcc ggccccgcca ggatctcggt tagccggatc
     2761 gagccggagc ggaaaacaaa cgcatgccct ccttcgagga ttctgatatc cgcttcccac
     2821 cccagttgct cgacgcgcct gaactcgcgg gtcgagaacc aatccggatg gacagcgcga
     2881 ttgaatacgc gaaacgcaag gtcagcgacg cgcgatctcc ccgagcttac tcccatgccc
     2941 tgcatttcct ttccggccat tgccccttgg cgccatagcg cgtgttggca attgatcatc
     3001 ccccttccgt gacgccgtgg gaacattata agtatcgatg cgatcccagg taaacggagc
     3061 cggggaacgt tgcgacaacg gaatttgttc aaacaccact caacacatcg gaaaccttat
     3121 tcttgccctc gcgtgggatc gatcactccg gcgggcacgt ggcgagaatc agcccgcctt
     3181 gaccgtcatg acaaggccac gtaaactctc gctcttgcct ggcatacggt ctaccaagga
     3241 aggtgtcatg gacttgatcg tacggaccga tccccctttg cccctgcgtg cctggaaggc
     3301 cctgcggtcg cgcggggccg gatatgcctg gcacaagttc ctccgccggt ctctgagcga
     3361 ttggccggcc tggaaacgac gaatcctata cgccgaccct cacaggtatt ggacgctgcg
     3421 cggcggtgat gattattttc gggaacagga aggccagaag gctcgaactc tgcgagcccg
     3481 atggcttgct gaacggctgg cacgatatcg acccacgtcg atcctcgagg tcggctgcgg
     3541 ctatggaaaa gtgctaggcg aactccgccg ccagcttgat ataccgctcg tcggtgtgga
     3601 ctttagccgg actcagctgg agcaagcttg tcgtttcctg gcaaacgatc gcgatatcga
     3661 acttctcttg agccgcggcg aacgcctgcc gttcgccgac cattccttcg acatggtcgt
     3721 gacctcggcc gtcattctgc acaacccgcc ggcgatcgcc gaacgcattc gccgggaggt
     3781 catgagagtg acgcgccggt tcgcggctca taacgaagag acgaacctga gttacaaccg
     3841 tttcggctac gataccgcgc tgtggtatcg ccggcagggc atcatcctgg ccgagtcggg
     3901 accgatcccg gtggatccgg atccatcggc ctctcagttt tgcgtcgctg tcatttaccc
     3961 tggcgaatga ttgaaggcga cgtccgcatt ccttcaagca aaacgaatca cacatgatcg
     4021 acctcggctt cgcggcgctc ctggcgctga ttgcagcggg cttcggcaag cgcatgctag
     4081 acgggatggg ccagtcgcca gagcatccgc tcgacgcgat ggccctcgcc ttgcccttgg
     4141 gcctggggct caccgccttg gcgacactcg ccctcggcga gctgggctgg ctcaattttg
     4201 tcggtttgtc ggtcctgcta gccgtgctcg tcgagctggg gatcatcgct tggttccact
     4261 tgttccgtga agttcgggcc tggttcaggg cgcgtgtccg cactcgcagc cggacatcat
     4321 tgtctctgtt cttcgccttc tgcctgggtc tggtgatcgt cgggacggca ctgaccgcat
     4381 tgacgccggt gaccgacggc gacgctctgt gctatcacct ccaggtgccc aaggtgtttc
     4441 tgatgcgcgg atcggtcggg ttttcgcccg acctccatga gacggtctac ccactcgtga
     4501 ctgagatgct ctacgcggta gcgctcgcgt ttcgcgggcc ggtcgcgtgc cgcggcatcc
     4561 agtgggtcct tggcctggtg ttcgcggcca atgtgacggc gctggcgcgc cccagtctcg
     4621 gccgccgcgc ctggtgggcc ggcgcgatcg cgatctcggt tcccgcagtg agtaacggca
     4681 tgtcggcccc gctcaacgat gtcgccctcg ccgcattcgg tacagccgct ttatttgcat
     4741 gggtgaggct acatgaacgg ccaaccgtct ccgcggcaat cgtggcgggg ctgttcatgg
     4801 gcctggcgat cggtgtcaaa taccctgccc tcgtcttgtg cggccttctg gccccgacaa
     4861 tcgcactgcg aacgatcgac gggcgagcga cgaacgccga cggcaccaga ccgcggtggt
     4921 tcaccctggc ggcaatcttt ttgacgacta cggccgcggt cggcggatgg tggtatttgc
     4981 gagcgtatat ccacaccggc aatccggttt atccattttt ccgcgacgtc ttcggcggcg
     5041 cgggactgga cgaggtgctc gacccgatca agcggccgct cacggtcacg ccgttcaatc
     5101 tgatcacggc tctggtgccc ctgtcgctgc atcccgagag gttcgacagc ttctcgcatc
     5161 aattcggccc gatgtttctg cttttcttgc ccgcggtctt cctgtttcga gctcccagac
     5221 gcgtgctggt attggtcggt ctagcctatg cgtttctgct cgtctgcctg acccagcgcc
     5281 agagcatgcg ctttcttttg atcgcactgg gaccgatgtc gatcggggtg gcgtacctcg
     5341 ccggcatctg gtgtaag
//
LOCUS       DATAQL010000566         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3264929, whole genome shotgun sequence.
ACCESSION   DATAQL010000566 DATAQL010000000
VERSION     DATAQL010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3264929"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VHS97_09800"
     CDS             <1..1482
                     /locus_tag="VHS97_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448535.1"
                     /translation="YFVAIPVGLVATGAFASFLTKGGMIRWLVAAVLQSLAFLCHLTA
                     AMVIAPAAALAYTSMVMHRRTARSSGAPLRLGGSAGRPVPGPTRRLTLMSHLAVWTIP
                     VIVLAINSFWWLPGIWLASTKGESGFAFFHPEGVGRRLIQIVVAESPVQSLLLACGLP
                     GLILLLKRDQPLGWALCGFCAAGMFWGYFAGGARALDFLQPGRHTYAFFTALAVAGGA
                     GIDEARRRARFGTHGVDYLDRWMLLGAILIVIRVWGYPLVESLRVRLTLGEPFLSSRP
                     SPRLLWIVERVKRHLQPGQRLLYEEGGKDLPGIRDPFEHGRFSGLLPELTGVEVIGGP
                     YLHASLTTNFTQFGEGKLFGRAEWDRTFFDRYAKLYRPSAIMCWSPHARRFCRENSDL
                     VQVLEDDGTIIFGRIVGFEGDFIEGSGRVEAMPGRIRVGEVSPGLDGNVLLRYHFVPY
                     LTTSPSVACEPEYREDDPVPFIRFRPPGGANGVDFELHLPVGR"
     gene            1577..2950
                     /locus_tag="VHS97_09805"
     CDS             1577..2950
                     /locus_tag="VHS97_09805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX3448536.1"
                     /translation="MPAIDSELEDHCRLERLSNWFFWSLWAMIILIAAAFYYEKAADH
                     RSAFVRWRPQVLQFWSGVNIYDKMIFPNPPIMPITLYPLMVLPTVAGAMCWFAIKVGL
                     TTAALVMLFQIVRPPGRLYPPMFRSLVLLLSLRPILGDLHHGNNNLLILFLVVAMFYT
                     WRRGYDVGAGLLLALATAYKVTPALFFVYFAYKRSWRTIGWGAVGLGLFLFVVPSAVI
                     GPQFNFECLRMWSHRMVTPFVVKGAFSPQEPNQSISGVIGRLLTDLHPGPGRYDTHFD
                     LNLISLPPWIVNYLIKGIAALLLGLLALLCRTRATDRRDPRLLGEIALVVLTMLFVSE
                     RSWKHHYVTVLIPYSYLVAEFFSSRIGPRSRMLLVGSWAVSFVLMAATSTELGGLFYD
                     GQGHEIAQGYGSFLWAGVVLYATVAWRVWVRRSETPVASDPTDPVSPSAKSHIALARA
                     PRSAVRS"
     gene            3121..>3568
                     /locus_tag="VHS97_09810"
     CDS             3121..>3568
                     /locus_tag="VHS97_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448537.1"
                     /translation="MSLSKELRRTRYRTFFTPEVLEDRLVLSAGEGSTFAIMPGQVTT
                     AGQVSTINFKIDPSMFTAPRKNGKIVLGVDVASAKSSSSSSTTSTLTPQIVSITDPSG
                     HTIRAQHTKYNPKVAKASKLGSSPTSAVTFSVNVPKTGQPVADYSIQ"
BASE COUNT          619 a         1019 c         1072 g          858 t
ORIGIN      
        1 tattttgttg cgatccccgt gggactcgtg gcgacaggtg ccttcgcaag cttcctgact
       61 aagggcggaa tgatccgttg gctcgtcgcc gctgtcctgc agagcctggc ttttctttgc
      121 catttgacgg ccgccatggt gattgccccc gcggcggctc tcgcttacac gagcatggtg
      181 atgcatcgcc gaactgcccg ctccagcggc gctccactgc gtctgggcgg atctgcgggc
      241 cgtcccgtcc ccggaccgac tcggcggctg accttgatgt ctcatctggc ggtatggacg
      301 attcccgtca tagtgctggc gatcaattca ttctggtggc tgccgggaat ctggctggct
      361 tcgacgaaag gagagagcgg cttcgcgttt ttccatcccg agggtgtggg tcgtaggctc
      421 attcagattg tggtggccga atcaccggtt cagagccttc tcctggcttg cggcctgccg
      481 ggactcattc tcctattaaa acgtgatcaa ccgctgggct gggcgttatg cggattctgc
      541 gctgccggga tgttctgggg gtacttcgcg ggaggtgcgc gcgcgctcga ctttttgcag
      601 cccgggcggc acacgtacgc tttctttaca gcgcttgccg tggcgggcgg ggcgggaatc
      661 gacgaagcgc ggcggcgagc gcgtttcggt acccacggag tggactacct cgatcgatgg
      721 atgcttttgg gtgccatctt gattgtaatt cgagtctggg ggtacccgct cgttgaatcg
      781 ctgcgggtcc gtctcacatt gggcgaaccg tttctgtcca gccgcccgtc gccgcgcctg
      841 ctctggatcg tcgaacgtgt gaagcgccat ctccaacccg ggcagcggtt actatacgaa
      901 gaggggggaa aggaccttcc tggcattcgc gatccgtttg agcatggacg gttcagcggt
      961 ctcttgccgg agctcacggg agtggaggta atcggcggcc cttatctcca cgcatcgttg
     1021 acaaccaatt ttacccagtt cggcgagggc aagctctttg gccgagccga atgggaccgc
     1081 actttctttg accgatacgc caaactgtac cggccttcag ccataatgtg ctggagtccg
     1141 catgctcgtc gcttttgccg cgagaattcc gacttggttc aggtgctgga ggatgacggc
     1201 acgattatct ttgggcgtat cgtcggtttt gaaggggatt tcatcgaagg gagtggacgg
     1261 gtggaggcga tgccggggcg aattcgtgtg ggcgaagtct cccccggact tgacggaaac
     1321 gtgttactgc ggtatcactt cgtgccgtat ctgacgacga gcccgtctgt cgcctgcgag
     1381 ccggagtatc gtgaggatga ccccgtccct tttattcgtt ttcgaccacc tggcggagcc
     1441 aacggtgtcg atttcgagct tcatctcccc gtgggacgat agcctctcgg ttttccggcc
     1501 gaacatcgcc ggctagacac agaaaacacg caagtaaatt gcgtgagcgt ggctctctcc
     1561 agggaagggg tatgccgtgc cagcgatcga ctctgaacta gaagaccatt gccgcctcga
     1621 gcggttatcg aactggttct tctggtctct ttgggcgatg atcatcctga tcgctgcggc
     1681 gttttactac gagaaagccg ccgatcatcg cagcgcgttc gtgcgctggc ggccacaagt
     1741 gctgcaattc tggtctgggg tcaacatcta tgacaagatg atcttcccca atccgccgat
     1801 catgccgatc acgctttacc cgctcatggt attgccgacg gtcgccgggg ccatgtgctg
     1861 gtttgcgatc aaggtagggc tgacgaccgc ggcgcttgtc atgctttttc agattgtcag
     1921 gccgccaggc aggctttatc cgccgatgtt ccggtcgctg gtgctgcttc tcagcttgcg
     1981 tccgatcctc ggtgatctcc atcacggcaa caacaacctt ttgattctct ttctggtcgt
     2041 cgcaatgttt tacacatggc ggcgtggtta cgacgtgggt gcggggcttt tgcttgcttt
     2101 agcgactgcc tacaaggtaa cgccggccct ctttttcgta tatttcgcct acaaacgatc
     2161 gtggcgaacg attggttggg gcgcagtggg gctgggcctg tttctttttg tggtgccaag
     2221 cgcggtcatc ggtccgcagt ttaacttcga atgccttcgc atgtggtctc accgcatggt
     2281 gacaccgttt gtggtcaagg gagcgttcag tccgcaagag cccaatcaat cgatttccgg
     2341 cgtaattggc cgactcctca ctgacttgca ccccggcccg ggccgttatg acactcattt
     2401 cgatctaaac ctgatttcgc ttccaccctg gatcgtcaat tacttgatca aaggcatcgc
     2461 ggcactcctt ttgggactgc ttgcgctctt gtgcaggacc cgggcaacgg atcggcgcga
     2521 tccgcggttg ctgggagaaa tcgcactcgt ggtgctgacg atgctgtttg tctcggagcg
     2581 gagctggaag catcattacg tcacggtgct gattccctac agttacctgg tggcggaatt
     2641 cttttcgtcg cgtatcggcc cgcgatctcg aatgctgctt gttggatcgt gggccgtatc
     2701 ttttgtcttg atggcggcca cctcgaccga actcggcggg ctgttctatg acgggcaagg
     2761 acacgagatc gcgcaggggt atggatcgtt tctttgggcg ggagtcgtgc tttatgcgac
     2821 cgttgcctgg cgcgtctggg tgcgtcgcag cgaaaccccg gtcgcatcgg acccgactga
     2881 tccggtctca ccgagtgcca aatctcatat cgccttggcc agagcacctc ggtccgcggt
     2941 caggtcttga aaagaaaata tcgcttattg ttggcgtggc agtaccgatt gtatcgataa
     3001 tagaagagta atcgaatccg gcgaccagga gggttgccct tcggaggact cttcctttcg
     3061 ggtcgatctt cgatcggccg attcctcacg tcccactcgg ttacaggcca aggagggccc
     3121 atgagcctgt caaaggaatt gcgccgcacg cgctatcgca cgttcttcac tcccgaggtc
     3181 ctggaagacc gcctggttct gagcgccggc gaggggagca cgtttgcaat catgcctggc
     3241 caggtcacca cagccggcca ggtctcaaca atcaacttca agatcgatcc gagcatgttc
     3301 accgcgccac gaaagaacgg aaagatcgta cttggggtcg acgtcgcctc cgcgaagtcg
     3361 agttcgagta gctcgaccac gtcgacgctc acgccccaga tcgtctcgat cactgatcct
     3421 tcgggtcata cgattcgggc ccaacacacc aagtacaacc cgaaggtcgc caaggcgagc
     3481 aagctcggca gttccccgac gtcggccgtc acgttctcgg ttaatgttcc gaaaacgggg
     3541 cagccggtcg cggactactc gatacagg
//
LOCUS       DATAQL010000567         7382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3415194, whole genome shotgun sequence.
ACCESSION   DATAQL010000567 DATAQL010000000
VERSION     DATAQL010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3415194"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..1888)
                     /locus_tag="VHS97_09815"
     CDS             complement(341..1888)
                     /locus_tag="VHS97_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3448538.1"
                     /translation="MTAILIASCFLLAQADGAPVVGPRSGSLVIDGGGEPPETARRFV
                     ELAAGPEYRAQTAKSAVDVFVKSHVDSSWEMARKIYDWAEVGYQEKRSAALLADAMEA
                     GGFKLERGVAGIPTSFIATIGSGKPVIGILGEYDALPGLSQQAVAERQARPDTTSGHG
                     CGHHLFGVASASASLALGEQIRAGALKGTLRFYGCPAEEGGSAKAFMVRAHLFDDCDA
                     VLHWHPSSQNIAGDEASQARIAVKFRFHGTSSHAAAAPEKGRSALDALELTNHAAELL
                     REHTPDFTRIHHVVTAGGSAPNVVPDFGEVFYYIRHPRSGVVQTLYPRLLKCAQAGAL
                     ATETRLETVYLGGILEIMPNDSLARVARANLAKLNDLSYDETESRFASQIQKTLTEPL
                     PLKSLSVIGERTGQGSGGSTDVGDVSWVVPTAGFTTACWVPGTSAHSWQAVAAGGTTI
                     GRKGMVLAARVLAATGCDLFQSPAVVAAARDELARRRAGRSYQSLLGTDQKPPLDYRQ
                     DPRQRDP"
     gene            complement(1953..3944)
                     /locus_tag="VHS97_09820"
     CDS             complement(1953..3944)
                     /locus_tag="VHS97_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX3448539.1"
                     /translation="MQGYQRILELFDAWDKRDVAIPFSELVESLRGLELERADLAGAL
                     VFADRNYRRIAIRRRPHYEALVLCWKSGQRSPIHNHLGSSCVVRVVEGRATETSYVPS
                     PCGKLVPLRSRTFAAGSVTGCRDAAIHQMANLEPSGQDLITLHVYSPPPSCWSYFTLD
                     VTTLADHDRLLHEGAETVVVNFGEAAPIAPEESFVEVIKSPSSIDMGPAIAIVGAGFS
                     GAMVAVHLARLAGPKAPRIVLFEKTERLARGIAYGTRCNQHLLNVPAGLMSALPDEPT
                     HFLDWLRAVDPTAHAGTFASRRVYGDYLEALLHSSAKTSSARIHFVRDEVVDMDIDNG
                     CTSVQLNTLNGDRLRADRVVLALGHPLPEEPQGLELASIGRGYVADPWSTGALSELAA
                     DAPIALVGSGLTAVDLIVEAHANGHRGIIYTISRHGLFPCRHQTSSGIPRPHIAISAD
                     SGATARGLLRHVRSEVAVCQAQGNDWRSVVDSLRPVTQTLWRSLGDRERARFVRHLAP
                     RWDVHRHRVAPHIDDVLQDARRSGRLVVVAGRVLSLADRNGTIELSFRRRGAPEAESL
                     YVHRVINCTGPARDVRLGSSNLLRSLIARGIGRPGPLALGLDVADSGALVRQDGGEHD
                     RIYAIGPLLKERLWETTAVRELRSQTLELARRLIAEPVI"
     gene            complement(4022..5176)
                     /locus_tag="VHS97_09825"
     CDS             complement(4022..5176)
                     /locus_tag="VHS97_09825"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX3448540.1"
                     /translation="MAAAEPAYGFSTRAIHAGQDPDPATGATVVPIYATSTFTQEAPG
                     RHKGYDYARSGNPTRAALETCLAALEGSEQALAFASGLAATTAVLSTLRPGDEVAAAA
                     DLYGGTFRLLERVFKPWGLVPRYTDDATPDGFARILSPHTKLFWIETPTNPLLQIIDI
                     AALAALAHDRGALLAVDNTFASPYLQRPIELGADLVVHSTTKYLAGHSDVIGGAVAGR
                     RDLLEPIAIYQNAAGAVPGPFDSWLVLRGVKTLSVRMERHCASARVLASWLAEHKQVA
                     HVYYPGLSIHPNHEVARRQMRDFGGMISVKLSGGAAAAERLLTRTRLFSLAESLGGVE
                     SLIGHPATMTHASIPADVRAARGVDDGLVRLSVGIEDVDDLRGDLEQALA"
     gene            complement(5264..6691)
                     /locus_tag="VHS97_09830"
     CDS             complement(5264..6691)
                     /locus_tag="VHS97_09830"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX3448541.1"
                     /translation="MRTRIHRAEHESAASLHAKVNSLRLGMSLTVLDAIGRTPLLPLH
                     RLSAGVPAKVLVKLESSNPGGSIKDRVGVAMVEEAERSGWLLPGGTIIEATAGNTGVG
                     LALAASVKGYRCIFVLPDKMSGEKIRLLKAYGAEIVITPTAVPPDSPESYNGVAERLA
                     REIPGAWRPNQFANLANPEIHYRTTGPEIWEQTGGRITAFVSGVGTGGTISGVGRYLK
                     ERNAAIKVIGADPEGSVLSGDAPKPWKVEGIGEDFVPKTLNGQVVDEWIRIGDAESFH
                     TARALARREGLLVGGSSGTAVAAALRYAQRLSADDVIVALCPDTGRNYLSKFYDDAWL
                     TQNHLHRGDPPAQTVGDLLRARGARTLVTVGPDTTAAEAALLLQASGISQLPVLRDGH
                     SVGSVQEVTLARLLHDDTDPGSVRVGDVMARPLPQLDVGVQLDEAYRLLLAGNSGVLA
                     VEGDSVVDIITRIDLIQYWTQRRGG"
     gene            complement(6852..7301)
                     /locus_tag="VHS97_09835"
     CDS             complement(6852..7301)
                     /locus_tag="VHS97_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3448542.1"
                     /translation="MSEHKVTVEWKRETPDFSYQSYNRDHDWAFDAGVTVRASAAPAY
                     LGNASCVDPEEAFVASLSSCHMLTFLAIASKKRFVLDEYHDEAVGILGNDTAGRLAII
                     KVTLRPRVQFSGEKAPTPEEVAQMHEQAHHACFIANSVKTEVIVEPR"
BASE COUNT         1371 a         2374 c         2325 g         1312 t
ORIGIN      
        1 gcagaacatc ttcgtgaggt tcaggccgtc ggcgagtcaa ttggatcgcc catctaaacg
       61 tgcggctatg gctggcgatc ttcgtaatcg agcaggtcgt tgggggtgga atgatttgtg
      121 aaccgctcga tctgctccta ccacggcggc ggcgcaaccg gtcgtggggt ggtgccgaac
      181 gatccaaagg attgtgaggc ccaccgcaag cgacggcctg gtcggtgggc cttgccgcga
      241 ttgatcgcgg ctcggcacct tcacacaaca ttccgacgac cgcgtcagcc ccgcccgccc
      301 atgatgcgcg ttcggcgcag aatccaacgg tggtagattc ctaagggtca cgctgccgtg
      361 ggtcttgcct gtagtctagg ggcggcttct ggtctgttcc aagcagcgat tggtacgacc
      421 ggcccgcgcg ccggcgcgcg agctcgtcgc gagcggcagc gaccacggcc ggcgattgaa
      481 ataggtcgca ccccgttgcc gccaggacac gggccgccag gaccatcccc ttgcggccga
      541 tcgtcgtacc cccggccgcg accgcttgcc atgagtgcgc cgacgtgccg ggaacccagc
      601 aggcggtcgt gaaccccgcg gtcggcacga cccacgacac atcgcccacg tcggtcgagc
      661 cgccactccc ctgcccggtc cgttcgccaa tgacggacag gcttttcaag ggcaatggtt
      721 cggtcagcgt tttctggatc tggctggcaa agcgactctc ggtttcgtca tagctcaagt
      781 cgttgagctt cgcgagattc gcgcgcgcaa cgcgcgccag cgagtcgttg ggcatgattt
      841 cgaggatgcc cccgagatag actgtctcca gacgagtctc ggtggcgaga gcgcctgctt
      901 gggcgcactt gagaaggcga ggatagaggg tttgcacgac tcccgaccgc gggtgcctga
      961 tatagtaaaa gacctcgccg aagtccggga cgacattggg agcactcccg ccggcagtga
     1021 caacgtggtg gattcgcgtg aagtccggcg tgtgttcacg gagcagctcg gccgcgtggt
     1081 tggtcagctc gagcgcgtcc agtgccgatc ggcccttctc gggcgccgcc gcggcgtgcg
     1141 agctcgtacc gtggaaccga aacttgaccg cgatccgcgc ctgactcgcc tcgtcgcccg
     1201 caatgttttg gctcgacgga tgccagtgga gcacggcgtc gcaatcgtcg aacaggtgcg
     1261 ctctgaccat gaacgccttg gcgctccccc cttcctcagc cgggcagccg taaaaccgta
     1321 gcgtgccctt gagcgcgccg gcccggatct gttcgcctaa agccagactc gcggaggccg
     1381 aggccacgcc gaacaggtgg tggccacagc cgtgcccaga cgtcgtgtcg ggccgtgcct
     1441 gtcgctcggc cacggcctgc tgcgacaggc cgggcaaggc gtcgtactca cccaaaatgc
     1501 cgatcacagg cttacccgag ccgatggtgg cgatgaacga agtggggatt cccgccacac
     1561 cgcgctcgag cttgaacccg cctgcctcca tggcatcggc cagcagagcc gccgaccgtt
     1621 tctcttgata gcccacctcg gcccagtcgt agatctttcg cgccatctcc caagacgaat
     1681 ccacatgcga cttgacaaac acatcaacgg cagatttcgc ggtttgcgcc cgatattcag
     1741 gcccagcggc gagctcgaca aaacgtcggg cggtctcggg aggttcaccg ccgccgtcga
     1801 tgaccaacga cccagacctg gggccgacga ccggtgcacc atccgcttgg gccagcagaa
     1861 aacaagaagc gatcaggatc gcggtcatga aagtctccgg atgcgataac aaagatctca
     1921 attcaaatat cgactggccg tgatgccgcg ggttagatca ccggctccgc gatcagccgc
     1981 cgcgccagct cgagcgtctg ggaccgcagc tcgcgcacgg ccgtcgtttc ccagagccgt
     2041 tccttgagca gcggtccgat cgcataaatc cggtcgtgct caccgccgtc ctggcgaacg
     2101 agtgctcccg agtccgcgac gtcgagtccc agagcgaggg gccccgggcg gccgatgccg
     2161 cgcgctatca acgatcgaag caggttcgat gagccgagac gcacgtcgcg cgccgggcca
     2221 gtgcagttga tgacacggtg aacatatagg ctctcggcct ccggagcacc gcgccgccgg
     2281 aacgacagtt cgattgtccc gttccggtct gctagcgata aaacgcggcc ggcgacgacg
     2341 acaagccgac ccgagcgccg cgcgtcttgc agcacgtcgt cgatatgcgg tgcaacacga
     2401 tggcgatgga catcccaccg cggagccaga tgccgcacaa accgcgcgcg ctccctatcg
     2461 cccagcgagc gccacagcgt ttgcgtcacc gggcgcagtg aatcgacgac ggatcgccag
     2521 tcgtttccct gggcctggca gacagccacc tccgaccgca cgtgccggag cagtccacgg
     2581 gcagtcgccc cggaatccgc cgagatggca atatggggac gaggtattcc agaagaagtt
     2641 tgatgcctgc aggggaagag cccgtggcgc gaaatcgtat agatgatacc gcggtgccca
     2701 ttggcgtggg cctcgactat cagatcaact gcggtcagcc ctgagccgac aagcgcgatc
     2761 ggtgcatcgg ctgccagctc gctcagcgcg ccggtcgacc aagggtcggc gacatatccc
     2821 cggccgattg acgcaagctc gagcccctgc ggctcttcgg gcagcggatg cccaagcgcc
     2881 agaaccactc gatccgcacg caggcgatcg ccattaagag tatttaattg cactgatgtg
     2941 caaccgttat cgatgtccat gtcgacgacc tcgtcgcgca cgaagtgaat ccgcgcgctc
     3001 gaagtcttcg cgctcgagtg aagcaaagct tcgagatagt cgccataaac acgcctggaa
     3061 gcgaaagtcc ctgcgtgagc ggtagggtcg acggctcgca gccagtcaag gaagtgggtc
     3121 ggctcgtcgg gcagggcgct catcaatcct gcgggaacgt tgagcaagtg ttgattgcaa
     3181 cgggtgccgt aggcgattcc acgtgccagg cgttccgtct tttcaaagag aacgatgcgc
     3241 ggggcctttg gtccggccag tcgcgccaaa tggacagcca ccatggcgcc actgaaacca
     3301 gcgccgacga tggctatcgc gggccccata tcgattgatg agggcgactt tatcacctcg
     3361 acgaacgatt cctctggcgc gatcggcgcg gcctcgccga agttgacaac caccgtttcg
     3421 gccccttcat gcaagagccg atcgtggtcc gccaatgtgg tcacgtcgag tgtgaagtag
     3481 ctccagcagg agggcggggg cgaatagacg tgcagggtga tcaggtcctg gccgcttggt
     3541 tcgaggttgg ccatctggtg gatggctgcg tcgcggcacc cggtcacgct tccggccgcg
     3601 aatgtccgcg accgcagtgg cacgagtttg ccgcacgggc taggaacata gcttgtctca
     3661 gtcgcgcgcc cctcgacgac tctgacgacg cacgatgaac ccaggtgatt gtgaatcggg
     3721 ctgcgctggc cgcttttcca gcagagcacg agcgcttcgt aatgagggcg gcggcggatg
     3781 gcgatgcggc gatagttgcg atcggcgaaa acgagcgcgc cggccaggtc ggctctctcg
     3841 agctcgagtc ctctgagcga ctcgaccagc tcggagaacg ggatggcgac gtcccgctta
     3901 tcccatgcat cgaaaagctc gaggattcgc tgataccctt gcatcgccgt cctccacacc
     3961 gcttatgcgc tttccgacac tccgcctttg gttcagatag ctttgagtga ggcgaaacca
     4021 atcacgcgag cgcctgctca aggtcgcccc gcaggtcgtc gacatcctcg atgcccacgc
     4081 tcagccggac caggccgtcg tcgacgccgc gggccgctcg aacgtcggcc gggatgctcg
     4141 cgtgcgtcat ggttgccgga tgaccgatca gcgattccac tcctcccaag ctttccgcca
     4201 ggctgaataa gcgcgtgcgc gtcagcagcc gctcggcggc cgcggcgcct ccgctcagct
     4261 tgacgctgat catcccgcca aagtcgcgca tttgacggcg ggcgacctcg tggttgggat
     4321 gaatcgacag accaggatag tatacgtgcg ccacttgttt atgctccgcc agccatgaag
     4381 caagcacccg agcgctggcg caatggcgct ccatgcggac cgacagggtt ttgacacccc
     4441 gcaagaccag ccacgaatcg aacgggcccg gaacagcccc ggcggcattc tggtaaatgg
     4501 cgatcggctc aagcaagtct cggcggccgg cgaccgcgcc gccgatgacg tcggagtgcc
     4561 ccgcaaggta tttggtcgtg ctgtgaacta ccaggtcggc gccgagctcg ataggccgct
     4621 gcaggtaggg ggaggcaaac gtattatcca cagccagcaa ggcaccccga tcatgcgcaa
     4681 gcgcggcgag cgcggcgata tcaatgatct ggagcagcgg gttggtcgga gtttcgatcc
     4741 agaagagctt ggtgtgcgga ctcaggatcc gcgcgaatcc gtcgggagtg gcgtcgtccg
     4801 tgtagcgagg gacgagtccc cagggcttga agacccgctc cagcaaccga aatgtgccgc
     4861 cgtagagatc ggcggccgcg gcaacctcgt caccgggccg caaagttgag agaacagccg
     4921 tggtcgcggc cagaccggag gcgaacgcaa gcgcttgctc gctcccctcg agtgccgcca
     4981 ggcaagtctc gagcgcggct cgcgtcgggt tgccgctgcg cgcgtagtcg taccccttgt
     5041 gacggccggg cgcttcctgg gtaaacgtgg aggtggcgta aatgggcacg acggttgcgc
     5101 cggtggccgg gtcgggatcc tggccggcgt ggatggcgcg ggtcgagaaa ccataagcgg
     5161 gttcggcggc ggccatgagg cattccctct ctgaaccaat tcggcatgag catcttgccc
     5221 gtggagttca acatccctcg agggccgcgc cgttcgggac cgttcagccg ccgcgtcgct
     5281 gggtccagta ctggatcaag tcaatccgcg tgatgatgtc gacgaccgaa tccccttcga
     5341 ccgcgagaac ccctgaattg ccggccagca gcagtcgata cgcttcgtca agctgaacac
     5401 cgacgtcaag ctgaggcaac ggcctggcca tgacatcgcc aacacgcaca ctgccgggat
     5461 cggtgtcgtc atgaagcaga cgcgccagcg tgacttcctg cacgctgccg accgaatggc
     5521 cgtcgcgcaa gacgggcaac tgcgagatgc cactggcttg caacagaagg gcggcctccg
     5581 cggcggttgt gtcgggcccg acggtgacga gcgtgcgggc cccgcgcgcc cggagcaggt
     5641 cgccgaccgt ctgcgccggc ggatcgcccc ggtggaggtg gttttgggtt agccacgcgt
     5701 cgtcgtaaaa tttgctcaga taattgcgtc ccgtatcagg acagagagcc acgatgacgt
     5761 catcggcgga gagccgctgg gcataccgca gcgcggccgc gacggccgtg ccggacgagc
     5821 cgccgaccaa aagcccttcg cgccgcgcca aggcgcgcgc cgtgtgaaag ctctcggcgt
     5881 cgccgatccg gatccactcg tccacgacct ggccattcaa cgtcttgggt acgaaatcct
     5941 cgccgattcc ctcgaccttc cacggcttgg gtgcgtcgcc cgaaaggacc gacccctccg
     6001 gatcggcccc gatgactttg atcgccgcgt tgcgttcttt caagtaccga ccgacgcccg
     6061 agatggtgcc tccggtcccg acgccgctga cgaatgccgt tattctgcca ccggtctgct
     6121 cccagatctc agggccggtg gtgcgatagt gaatctcggg gttcgccaga ttggcgaatt
     6181 ggttgggccg ccatgccccg ggtatctcgc gcgccaagcg ctcggcgacc ccgttatagc
     6241 tttcaggcga gtcgggcgga acggctgtgg gcgtaataac gatctccgcg ccataagcct
     6301 tcaagagccg gatcttctcg ccactcatct tatcgggcaa gacaaagatg cagcgatatc
     6361 ccttgaccga agccgccagt gccaggccaa cccccgtatt gccggccgtg gcctcgataa
     6421 tcgttccgcc aggcagcagc cacccgctgc gctcggcctc ctcgaccatg gctacgccca
     6481 cgcggtcctt gatgctgccc cccggattac tcgattcgag cttgaccagc accttggccg
     6541 gcacccccgc gctcagccga tgaaggggta agagcggcgt tcggccgatc gcgtccaaga
     6601 cagtcaggct catacccagt ctcaggctgt tgaccttggc gtgaagcgat gctgccgact
     6661 catgttcggc gcgatgaatc cttgtccgca tacgagcctc cgtgctcgat gtttcttgcg
     6721 cgagatactg gccgaataag gtcgggacgt acggccagac tccccggcgt cccctgagta
     6781 tcggatttca cccaaatccg tcaagccgag ctttttgagt gatgagtcat caaaagcaag
     6841 ttctgaccac atcaccgcgg ctcgacgatc acttcggtct tcaccgagtt ggcgatgaaa
     6901 caggcgtgat gggcttgttc gtgcatctga gcgacttcct ctggggttgg agccttctcg
     6961 ccgctgaact gcacccgcgg ccgcaacgtt actttgataa tcgccaaccg gcccgccgtg
     7021 tcgttgccga ggatcccgac ggcctcatca tgatactcgt ccaggacgaa ccgcttcttg
     7081 gacgcaatgg ctagaaacgt cagcatgtgg cagctcgaga gactggcgac gaacgcctct
     7141 tcaggatcga cgcacgaggc attgccgagg taggccggcg cggccgaggc gcggacggta
     7201 acgcccgcat cgaaggccca atcgtggtcc cggttgtagc tctggtaaga aaagtcgggg
     7261 gtttcgcgct tccactcgac cgtgactttg tgctcggaca tgacgctcag tctccaatac
     7321 atgttcaaat gattgtcaca ccaatcagtt agaatcggcg tgtccgggct gtcacattaa
     7381 tt
//
LOCUS       DATAQL010000568         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1738219, whole genome shotgun sequence.
ACCESSION   DATAQL010000568 DATAQL010000000
VERSION     DATAQL010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738219"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VHS97_09840"
     CDS             <1..390
                     /locus_tag="VHS97_09840"
                     /inference="COORDINATES: protein motif:HMM:NF023785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448543.1"
                     /translation="NHSGSTKSYDYVREHNEALNRIDFIETRDEITTEYDPGTLTTVQ
                     QHDGSILRLRKLGEEYDPSDKIAAMKRVQESLASGEVVTGLLYVDPTPKDLHHHLNTI
                     EAPLNTLDTPDLCPGPSALDRINASLR"
     gene            473..1489
                     /locus_tag="VHS97_09845"
     CDS             473..1489
                     /locus_tag="VHS97_09845"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX3448544.1"
                     /translation="MLDFLELFAHVLASPFKSQARLEAEIVVLRQQLMVLRRRVPSKP
                     KLTAVDRLLFVWLYRLFPSILNAITIIQPETVVRWHRSGFRLYWRWKSRSRGGRPRVP
                     AEIRRLIREMSLANRLWGAPRIHGELLKLGIEVAQSTVAKYMARSGRGPSQTWKTFLR
                     NHAAGIGAMDFLIVPTTGFRLLFVLVILRHQRRRLISLSVTPNPTAQWIAGQITEAFP
                     WDEAPDYLIRDRDASYGHAVTRRLAAMGIRDHPTAARSPWQNGHAERLIGSIRRECLD
                     HIVVFGEAHLRRILAAYVLYYNDLRTHLSLDKDTPSHRPIRRLGQVAAQPILGGLHHE
                     YCRM"
     gene            complement(1468..>1922)
                     /locus_tag="VHS97_09850"
     CDS             complement(1468..>1922)
                     /locus_tag="VHS97_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448545.1"
                     /translation="WTRHEAGASNAGPLPECVPIGYTVLALRAPRAKTAYIDGELCAL
                     RADGVPSFGLLQAAMDQGRTNSLVFFAFDLLYLDGKSTAALPLIERKQRLHPLFALAV
                     PGLHFTDHVVGNGPGRPQACLRDGSRGRHLETGRAALVPAEYYIRQYS"
BASE COUNT          360 a          612 c          590 g          360 t
ORIGIN      
        1 aaccattcgg gctcgaccaa gagctacgac tacgtgcgcg agcacaacga ggcgctgaac
       61 cgcatcgact tcatcgagac ccgcgacgag atcaccaccg agtacgaccc cggcaccctc
      121 acgacggtgc agcagcacga cggctcgatc ctgcgcctgc gcaagctcgg cgaggagtac
      181 gatccctccg acaagatcgc ggcgatgaag cgcgtccagg agagcctggc ctcgggcgag
      241 gtcgtgaccg gtctgttgta cgtcgatccg acgccgaagg acctgcacca ccacctcaac
      301 acgatcgagg cgccgctcaa cacgctcgat acgcccgacc tttgccccgg cccgtcggcg
      361 ctcgaccgca tcaacgccag cctgcgctag tccgcgccgg caatcctctt gtccataccg
      421 agaactccat ccgagctaga ttagcgggcg agatcggtta ggctgatggg cgatgctcga
      481 cttcctggag ctgttcgccc acgttttggc ttcgccattc aagtcgcagg cgcgactcga
      541 ggcggagatc gtcgtactgc gacaacagtt gatggtgctg cgtcgacggg tgccgtcgaa
      601 gccgaagctc acagcggtag accgactgct cttcgtctgg ctgtatcggc tgtttccgtc
      661 gatattgaat gctatcacga tcatccagcc cgagactgtc gtgcgttggc accggtcggg
      721 ctttcggctg tactggcgct ggaaatcacg ctcccgtggc ggtcggccaa gggttccagc
      781 agagatccgg cggctgatcc gggagatgag cctagccaac cggctgtggg gcgccccgcg
      841 catccatggt gaactgctga agctcgggat cgaagtcgct cagtcgacgg ttgccaaata
      901 catggcgagg agtgggcgag ggccgtcgca gacttggaag acctttctcc gcaatcatgc
      961 ggccggcatt ggcgcgatgg actttctgat cgtgccgacg actggcttcc ggttgctctt
     1021 cgttctggtc attctcaggc accaacgccg gcggctgata tctcttagcg tgacgcccaa
     1081 tcccacagcg cagtggatcg ccggccaaat cactgaggcg ttcccttggg acgaagcgcc
     1141 tgactatctc atccgggatc gcgacgcgtc ttacggccat gcagttacca ggcgtctggc
     1201 agccatgggc atccgggatc acccgacggc agcgcggtca ccgtggcaga atgggcatgc
     1261 ggagagactg atcggctcga tccgccggga atgcctcgat cacatcgtcg tcttcggcga
     1321 ggcccatctg cgccggattc ttgctgccta cgttctttat tacaacgatc tccgaacgca
     1381 tctgtcgctc gacaaggata cgccgagcca tcgaccgatc cggcggctcg gccaagtcgc
     1441 cgctcagcca atcctcggtg gacttcatca tgaatattgt cggatgtagt attcagcagg
     1501 gacaagggct gctcgacccg tttcgagatg acgccctcga gacccatctc gcaagcatgc
     1561 ttgcggacgc ccggggccat tgcccacgac gtggtcggtg aagtgcaatc caggaaccgc
     1621 cagggcgaag agcggatgca ggcgttgctt gcgttcgatc aacggcagcg ccgcagtgct
     1681 cttgccgtcc agatagagga ggtcgaaggc gaagaagact aggctgttcg ttcgtccctg
     1741 atccattgcg gcctgcaaca gaccgaacga tggcacgccg tcggcgcgta gcgcgcagag
     1801 ttcgccatcg atgtaggccg tctttgcccg cggcgcgcga agggctaaca cggtgtagcc
     1861 aatcggcacg cattcgggca acggccccgc gttgctcgcg cccgcttcgt gacgggtcca
     1921 ct
//
LOCUS       DATAQL010000569         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_819582, whole genome shotgun sequence.
ACCESSION   DATAQL010000569 DATAQL010000000
VERSION     DATAQL010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819582"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="efp"
                     /locus_tag="VHS97_09855"
     CDS             <1..605
                     /gene="efp"
                     /locus_tag="VHS97_09855"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX3448546.1"
                     /translation="SLKRTARGDLRTAMMLKATDIRRGMVITIEGVNFVVLDFAHHTP
                     GNLRAMVQTKLRNMNNGALIEKRLRSVDQIEVPYVETKEYEYLYSAGDDHVFMDTETY
                     DQLSFSPEIIGTAMDYLLPNSRVMVKYINEKPVSIEVPDFVELSVIDTPPSLSGATAT
                     NQYKEATLETKLKVQVPPFVKPGEKIRIDTRTGEYLERVK"
     gene            645..1787
                     /locus_tag="VHS97_09860"
     CDS             645..1787
                     /locus_tag="VHS97_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX3448547.1"
                     /translation="MTRLLSSLVAIPSVNPMGRHLAGPEYLETRLANYLEDWFRELGV
                     RCERQPVGPGRDNLLAWYIAPDARRLILYDVHQDTVPADGMTIAPFEPQISQGRLSGR
                     GACDVKGSMAAMLAAFARLVRERPRGSASVLLACTVDEEFTHTGSSRLAEMPHGAELA
                     IVAEPTLLDLVHCHKGALRWKIRTNGVACHSSTPDLGVNAIYRMGRVLDLLAKYAGEL
                     AASAPDPILGPPTLSVGRIEGGQSVNVVPDWCEIEVDRRLIPGEDAFASMEAVRKLAE
                     SLFDSSNCFLMSQPWVHMPPLEIRATRWIEPLSDAIFMATGRRPDVRGVPFGTDAGPL
                     SENGTPCVVFGPGDIAQAHTKDEWIELDQVERASQAYFQIAVELGR"
     gene            complement(1933..2274)
                     /locus_tag="VHS97_09865"
     CDS             complement(1933..2274)
                     /locus_tag="VHS97_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448548.1"
                     /translation="MPRFSWIKISALTFALAACGGVNTAQAQDWTNYLHWPYVPPQVP
                     GNGFEYNGLYDGWYRYPREQRIVPQIQGPYYRNEYGGYRVFGIFRHPNGWHEWDKKRW
                     YKGYHHVLDVF"
BASE COUNT          497 a          694 c          750 g          464 t
ORIGIN      
        1 gctctttgaa acgaacagcg agaggagact tgcgaaccgc catgatgctc aaagcgaccg
       61 acatccgtcg aggtatggtg atcacgatcg aaggtgtgaa cttcgtcgtc ctcgactttg
      121 cacaccacac gccgggcaac ctccgggcaa tggtccagac caagctccgg aatatgaaca
      181 acggcgcctt gatcgagaaa cggctgcggt ccgtcgacca gatcgaggtt ccctacgtcg
      241 aaaccaagga atatgaatat ctgtactcgg ccggcgacga tcacgtattc atggacaccg
      301 agacctacga tcagctcagc ttctcgccgg agatcatcgg cacggcgatg gattacctgc
      361 tgcccaactc gcgggtgatg gtcaaataca tcaatgagaa accggtatcg atcgaagtcc
      421 ctgactttgt cgagctgtcg gtgatcgaca cgccgccgtc cctctcgggc gcgaccgcga
      481 ccaatcagta caaagaagca actctcgaga cgaagttaaa agttcaggtt cccccgttcg
      541 tcaagccggg tgaaaagatc cggatcgaca cgcggaccgg cgagtacctc gagcgagtca
      601 agtgacgaag aggcccagag caggagagct gcgccttgga tcaagtgact cggttgcttt
      661 cgtccctggt cgccatcccg agtgtcaacc cgatggggcg acacctggcc gggcccgagt
      721 atctcgagac ccggctggcg aactatctcg aggactggtt tcgcgagctg ggggtccgat
      781 gcgagcggca gccggtggga cccggccgcg acaacctgct ggcgtggtat atcgcgcccg
      841 acgcgcggag gttgatcctc tacgacgtcc accaggatac ggtgccggcc gacggcatga
      901 cgatagcacc gttcgaaccg cagatttccc agggacggct gtcggggcgc ggggcgtgcg
      961 acgtcaaggg atcgatggcg gcgatgctgg cggccttcgc acgccttgtg cgagaacggc
     1021 cgcggggatc ggcttcggtg ctacttgcct gcacggtcga tgaagaattc acgcacaccg
     1081 gctcatcgcg actggcggag atgcctcacg gcgccgagct ggcaattgtc gccgagccga
     1141 cgcttctaga cctggtccat tgccacaaag gggcgttgcg ctggaaaatc cgcacgaacg
     1201 gcgtggcctg ccacagctcg acccctgacc tgggtgtcaa cgcaatctac aggatgggtc
     1261 gtgttctgga tcttctcgcg aaatatgcgg gcgagcttgc ggcctcggca cctgacccga
     1321 ttctcgggcc gccgaccctg tcggtcggtc gaatcgaggg gggacagagc gtgaacgtcg
     1381 tgcccgactg gtgcgaaatc gaggtcgacc gccggctgat cccgggtgaa gacgcttttg
     1441 cctcgatgga agccgtgaga aagctggcgg agtcgctgtt tgattcgtct aattgctttc
     1501 taatgagtca gccatgggtg cacatgccgc ctttagagat ccgagcgacg cgctggattg
     1561 aacctcttag tgacgccatc ttcatggcta ccggacgtcg tcccgatgta aggggagttc
     1621 catttggtac ggatgccggc ccccttagtg agaacggcac tccatgcgtg gttttcggac
     1681 ctggcgacat cgctcaggcg cacacgaagg atgagtggat cgagctcgac caagtggagc
     1741 gcgcgtcaca ggcgtacttc cagatcgccg tagagctcgg ccgataggct tatagcgcag
     1801 tccgaacgac gacaaggccc cggccatcct cggagggaga ggacgaccgg agcccgggcc
     1861 gggctaagtc aaattagggg tccgctggag ccggccggaa tgcgtcgttg ccaatgtttt
     1921 ggtcggcggt tttcagaaga catccagaac gtgatggtag cccttgtacc atcgcttctt
     1981 gtcccattcg tgccaaccat tgggatggcg gaaaataccg aacacgcgat atccgccgta
     2041 ctcgttgcga tagtacgggc cttgaatttg gggcacgatc cgctgttcac gcggataacg
     2101 ataccagccg tcgtagagtc cgttatactc gaacccgttg cccgggactt gaggcggaac
     2161 gtacggccag tggagatagt tggtccagtc ttgcgcctgg gccgtgttaa cccccccgca
     2221 cgcggccagc gcgaaggtca gggctgatat tttgatccat gagaagcgcg gcacaataca
     2281 cctccgtgta gggaaccctt tcccaaaccg tggctactat gccacacaat taacgagtgg
     2341 gcggcagatt ttcgattttc cgcgcggcta tggaggcgcg ttatgacgga catacggatt
     2401 tgcgg
//
LOCUS       DATAQL010000570         4858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_974026, whole genome shotgun sequence.
ACCESSION   DATAQL010000570 DATAQL010000000
VERSION     DATAQL010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974026"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VHS97_09870"
     CDS             <1..497
                     /locus_tag="VHS97_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3448549.1"
                     /translation="TVPLIEAAYEPYHPKKDVEKTVPYDLLLQAEKQARQFTIILGTI
                     AAISLLVGGIGIMNIMLATVTERTREIGIRRALGAKRRDITQQFLIETVVLAGVGGML
                     GVLLGFTIPMVIIYFIPDQKAVVTGASVMLSFSISVAVGILFGIYPAARAALMDPIEA
                     LRHE"
     gene            1038..1517
                     /gene="greA"
                     /locus_tag="VHS97_09875"
     CDS             1038..1517
                     /gene="greA"
                     /locus_tag="VHS97_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX3448550.1"
                     /translation="MSTDRIPMSKEGYEKLKAQLDKMKNEDMPRIAEQIAQARGYGDL
                     SENAEFDAAVEAQGMLQARINDLQNKLGSAYLVDKSNLSTDRAAYGLKVRVVDLDINE
                     EEDFILVGPGEEDYDQNKILITSPIGQGLVGKQLGEQCEVQIPRGVLRLKILDISVA"
     gene            complement(1546..2313)
                     /locus_tag="VHS97_09880"
     CDS             complement(1546..2313)
                     /locus_tag="VHS97_09880"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX3448551.1"
                     /translation="MNDPLVILHEDAHCLAVVKPPGQFTQGEWAPPDEITLETAVRRY
                     LDPADPRAVYLGIVHRLDRPTSGLLIWAKTAKAARRLASQFELRKVFKEYWAVVESGH
                     RQTDDDACGHDQTWTDWLTRPDRSGIVRAALPHATGAREAITAVSFGTALTLPPLCRW
                     LRLWPQTGRTHQLRAQAARRGMPILGDRVYGSTAASPLSQGIALHARVLAFYHPVTGS
                     PMRLIAPIPGSWKEAGILLPEPPIAESLESMPERPTV"
     gene            complement(2404..2772)
                     /locus_tag="VHS97_09885"
     CDS             complement(2404..2772)
                     /locus_tag="VHS97_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448552.1"
                     /translation="MDRHNRVLVSGSLAVLLGFLITGSGCRSMRNDVPPGKPYSTTGG
                     SPPPLGFNSDPHPNTSVGGGMYGGGLTPGSSSPSGGPAGAGGVPQLGTPTPGTGPYGA
                     PTSNQYGPLSGTASPAPLNR"
     gene            complement(3063..3680)
                     /locus_tag="VHS97_09890"
     CDS             complement(3063..3680)
                     /locus_tag="VHS97_09890"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX3448553.1"
                     /translation="MAEYTLPPLPYDYGALEPHIDAKTMEIHHTKHHQTYITNLNNAL
                     KEHAGLQGKKIEDLIAHLDAVPEAIRTAVRNNGGGHANHSLFWQIMKPGGGGDPTGAL
                     AQAITSELGGFPAFKEAVGKAALARFGSGWAWLIVGKDGKLAVTSTPNQDSPIMDGTT
                     PLLGVDVWEHAYYLKYQNKRADYVAAWWNTLNWDEIGQRYKAAKK"
     gene            complement(4347..4772)
                     /locus_tag="VHS97_09895"
     CDS             complement(4347..4772)
                     /locus_tag="VHS97_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448554.1"
                     /translation="MPDDPDPPDPPDPPDPPDPPDPEDPPEPEPPPEDIPPDAPDDEE
                     LPPLELKLDDEEDDEVDDDPPLFVLPDFEEEEEEEPLFLVLDLLLLLVLLLASWFWPL
                     PLPPVPDPLWLPLAPPEPEPWPWLDEPLPVVPLPLDVPP"
BASE COUNT          988 a         1411 c         1487 g          972 t
ORIGIN      
        1 tgactgtacc cctgatcgaa gccgcctacg agccctacca tcccaagaaa gacgtcgaga
       61 agacggtccc ctacgacttg ctgctgcaag cagagaaaca ggcacgccag ttcacaatca
      121 ttttggggac gatcgcggcg atctcgctct tggtgggtgg tatcggcatc atgaacatca
      181 tgctggcgac agtcaccgag cggacgcgcg agattgggat tcgtcgggcc ctgggagcaa
      241 agcgacgtga catcacccag cagttcctga tcgagaccgt cgttctcgcc ggagtcgggg
      301 ggatgcttgg cgtcttgctc gggttcacca tcccgatggt gatcatctac tttatcccgg
      361 accaaaaagc ggtcgtcacg ggagcctcgg tcatgctttc gttcagtatc tcggtcgccg
      421 tcggcatctt gttcggcatc tacccggccg cacgggccgc cctgatggat cccatcgagg
      481 cgctacggca cgaatgactg gcaagcgcac tcgatctcac acggcttcgt tactacgcat
      541 ttggcgattc catgattgaa cagcacgccg ggagggcaaa gttgattcgg ttgctggcga
      601 ttgggcaacg gggattttga aggtcctgat tccgagaggg aatcgccgcg gagcgttcga
      661 tcgcacttgg ccttgcgtcg aagagcctcg aaggctaggc tagtttttcc atagatatgg
      721 atgaccgcgg cttcgggctt cccgatgtcg cggtttttgt tctagatgtc attcggccgg
      781 gaacagcgca tccgacgacg atcatttccc ggctgcggag acttctctgt atcctggttt
      841 ctgagataag gacgaccctc gccaccacga cacccgtctc aaccggcctc cggtcaaacg
      901 atttccggtc tccaagccgt gttaaatcga agcatcccac gcccagccgt taacgccagg
      961 tgggccctca tgtgctttgg gtggtgtccg gtttcaagtc gatgagggtc gagtgacaga
     1021 ttggggagca cacccgcatg tccacagatc gaatcccgat gtcgaaggaa ggctacgaaa
     1081 agctcaaggc ccagctcgac aagatgaaaa acgaggacat gccccggatt gccgagcaaa
     1141 ttgcccaggc ccggggatac ggcgacctgt ccgagaatgc cgagttcgac gcggctgtcg
     1201 aggcgcaggg gatgctgcaa gctcggatca acgacctgca aaacaagctt gggtccgctt
     1261 acttggtcga caaatcgaac ctctcgactg atcgagccgc ctacggccta aaagtgcgcg
     1321 tggtcgacct ggatatcaat gaagaggaag acttcattct cgtcggtccc ggcgaagaag
     1381 actacgatca gaacaagatc ctgatcacca gtccgatcgg ccagggactg gtgggcaagc
     1441 aacttggcga gcaatgcgaa gtccagattc cgcgaggagt attgaggctc aagatcctcg
     1501 acatcagcgt ggcctaaaac gcatggcgtc tgactgctcg aaccgtcaga ccgtgggccg
     1561 ctctggcatg ctctcgagag actcggcgat cggcggctcg ggcagaagga tccctgcctc
     1621 cttccatgac ccggggatcg gtgcgatgag ccgcatcggg ctgccggtaa ccggatgata
     1681 aaacgccagc acgcgggcat gcaacgcgat accttgcgac agcggagagg ccgcggtcga
     1741 tccgtagact ctgtctccca gaatcggcat accccgtcgg gccgcctgcg cccgcaattg
     1801 atgtgtcctg ccggtctggg gccaaagccg caaccatcgg cataaaggag ggagggttaa
     1861 agccgtcccg aaactcactg cggtgatcgc ctcacgtgcc cccgtggcgt gtggtagcgc
     1921 ggccctcacg attccagaac ggtcggggcg tgtcagccag tcggtccagg tttggtcgtg
     1981 accacaagcg tcgtcgtctg tttgtctgtg gccagactcg acgaccgccc agtattcttt
     2041 gaaaaccttt cgcaactcga actgagacgc gagccggcgg gcggctttgg cagttttggc
     2101 ccaaatcaag agaccggatg tcggccgatc gagccggtgg acaatgccga gatagaccgc
     2161 gcggggatcg gccggatcaa gatagcgcct gaccgccgtt tccagcgtga tctcgtccgg
     2221 cggcgcccat tctccctggg tgaactggcc cggcggcttg acgacggcca agcaatgagc
     2281 gtcttcatga agaatcacga gcgggtcgtt cacgcgatgc tcaggtcgtc agccgggggc
     2341 gggtggagtg acccgacggc cacacgccat caactggctg gcgccgggtc gagtttgtca
     2401 gatttaccga ttgagcggcg ccgggctcgc ggtccccgaa agcggaccat actggttcga
     2461 ggtgggtgcg ccatacggcc ccgtgcctgg agtgggggtt ccgagttgtg gcacgccacc
     2521 agcgcccgcc ggaccgccgc tgggcgaaga tgatcccggc gtcaatccgc caccgtacat
     2581 accgcctccc acagacgtat tgggatgagg atccgaatta aacccaagcg gcggagggct
     2641 gccccccgtg gtcgagtaag gcttgccggg cggaacatcg ttccgcatgc tgcgacagcc
     2701 tgatcccgta ataaggaacc ccaaaagcac ggccaaagac ccgctgacca gcacgcgatt
     2761 gtgtcgatcc atcgacctga cctcctcatt ccttggcgcc cgacaccacc cggcatccag
     2821 gcgaaaatga gcacttgctc gctcttaccg agcgtcgcgt ttgacgcccg tcccaaaccc
     2881 atttcccgac aggacgatca attgcttctt gcgagatggt gcggatcgcg cggaaacggg
     2941 agaacagagg cctcggaagg gtgctaatcc cttccaaggc gcaggcatca tagcggattc
     3001 gtcagcgcgg tcaagtgcca atgttgcaag gtaaggcgaa acggtcggtc tcagatgatg
     3061 agctatttct ttgccgcttt gtaacgctgg ccgatctcgt cccagttgag ggtattccac
     3121 caagcggcga cgtagtcggc acgtttgttt tgatacttga ggtagtaggc gtgctcccag
     3181 acgtcgacgc cgaggagtgg cgtcgtaccg tccatgatcg ggctatcctg attaggagtc
     3241 gaagtgacgg cgagcttgcc gtccttgccg acgatcaacc aggcccatcc cgagccgaat
     3301 cgcgccaggg ccgccttgcc aactgcttcc ttgaaagccg ggaacccccc cagttccgac
     3361 gtgatggctt gtgcgagtgc cccggtcgga tcgccgccac cgccgggctt catgatctgc
     3421 cagaacagcg aatggttggc gtgccccccg ccgttattgc gcacggctgt ccggatggcc
     3481 tcgggaaccg catcaaggtg cgcgatcaga tcctcgatct tctttccctg gagcccagcg
     3541 tgttccttga gtgcattgtt gagatttgtg atataggtct ggtggtgctt cgtgtggtgg
     3601 atctccatcg tcttggcgtc gatgtgcggc tcaagcgcac cgtagtcgta cggcaaggga
     3661 ggcaaggtgt attcagccat ggtatcgtcc tcaaaaattc cttcgtgtcg ccagaccccg
     3721 cactgcaatg ttgcagtcga ttcaccgagc aaatcgagtg ccgcaaccgc ggtgcgagga
     3781 acgttctccg tgccgtcaaa ctaccacacg aagagccgct tcttccagaa ggggcctggt
     3841 gaacctttcc tgcctgcgaa acctaaggca gtccatagag cgatgatttg tcgctgataa
     3901 cttatggtga aatacatcga ggagattcct acggcatcac cagctgcttt tgcgaacgtc
     3961 acctgattga ccggtggtgt cttttgccga taatggtatt tgctcacaag cctgacggtt
     4021 tcttatccgc tgccgcggag cccatttatg ttgcctgaat ccgatcaatt cgacttcaaa
     4081 ccgatcgaaa tcccccaggt gcgtcgcgaa tactggggtt atgtcaaaat tgcggcggcg
     4141 cttgtcgcgg tctcgggtgg ggcttgggcc tggcagagcg gctatcgccc ggcctttctc
     4201 gagtcggcaa agttcccgct ggaattcgtc gagatcgata aaggggatgt cgacattgtc
     4261 gtcgtagagc aagggacggt ggagagtgcg aataacacca ctgtgcgctg tcaagtcgaa
     4321 gccctgatcg ggaccgttgg cggcactcag ggggggacgt ccaaggggag cggcacgaca
     4381 ggaagcggct catccagcca gggccagggc tcgggctccg gaggcgccag cggcagccag
     4441 agcggatcgg gaaccggcgg gagcggaagc ggccaaaacc aggacgcgag cagcaagacc
     4501 aagagcaaaa gtaaatccaa gaccaagaaa agtggctcct cttcttcttc ttcttcaaag
     4561 tcgggcagca caaagagtgg tggatcatcg tccacctcgt cgtcttcctc gtcgtctagt
     4621 ttgagttcaa gcggcggaag ctcttcgtca tcgggcgcgt ccggtggtat gtcttccggc
     4681 ggcggctcag gctcgggagg atcttctggg tcggggggat ctgggggatc cggaggatcc
     4741 gggggatcgg ggggatcggg atcgtcgggc acaacgacaa catctgtaaa gccaacaatc
     4801 cgcagcttta gctacgaggt gatcgctcac gtccccttgc gtcctgtaac gaccaagt
//
LOCUS       DATAQL010000571         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1432273, whole genome shotgun sequence.
ACCESSION   DATAQL010000571 DATAQL010000000
VERSION     DATAQL010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432273"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VHS97_09900"
     CDS             <1..367
                     /locus_tag="VHS97_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448555.1"
                     /translation="VTPPSVRFLGPKVYLGAIVILISAMRQGPSPRRVRELSTRFGAD
                     RRTIARWQVFWRECFPHTPFWKVARARLVPVVKIVSLPYSLVDAFLCRHPPCRGWTLL
                     LRFLSPITVPGGLRIEVAR"
     gene            464..1552
                     /locus_tag="VHS97_09905"
     CDS             464..1552
                     /locus_tag="VHS97_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEX3448556.1"
                     /translation="MTQPDSCSTGALWARFRFSVVGPLLSSPPARGTLRTAIRSLAEK
                     TWAHPVTGRDVRFTAVSIERWYYTARRQRDDPVGALRRAVRKDSGKISLTAALAERLH
                     LQYREHPHWSFQLHYDNLAALVKADPTLGKLRSYSTVKRYMRAHGLVKRPTVQLHQGP
                     GEARAERRRQTHEVRSYEAQYVGALWHLDFHHGSLKVLTPGGQWQRPIALAILDDHSR
                     LCCHVQWYLSETAEDLVHGFSQAIQKRGLPRALLTDNGSAMVAEEFTEGLLRLGIVHE
                     RTLPYSPYQNGKQEAFWGTLEGRLMKMLDGVAELTLDLLNTATQAWAEIEYNHAAHRE
                     TSCSPVERVGRAPDVLRLTCISHPYISE"
     gene            complement(1766..3277)
                     /locus_tag="VHS97_09910"
     CDS             complement(1766..3277)
                     /locus_tag="VHS97_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX3448557.1"
                     /translation="MTECKSKADRKATPWRRRRIDRRPPRVEIPLVGANVTYLDFPVL
                     GSRQVVAAFDGGDISSDGGSLLLRKTEELTAIIRQFAECFIDHRDPELIEHSLVHLVA
                     QRVYALALGYEDLNDHDGLRHDPLLATIVGKKDPTGQSRLRKRDKGKALAGKSTLNRL
                     ELTPVGADEDSRYKKITCNTRAVEDLFVELFLQAYPVPPDRIVLDLDATDDPVHGHQL
                     GRFFHGYYKHYCFLPLYIFCGDHLLCARLRPSDIDASAGALKYLQRIVTRIRQSWPHV
                     KIVVRGDSGFCRDQIMAWCETSSIDYIFGLAKNSRLLTMIAGESEQARVEYETTKQPT
                     RVFAELRYETRNSWSRERRVVAKAEHLDKGANPRFVVTSLDREQYPAQELYERDYCSR
                     GEMENRIKEQQLHLFADRTSAGTMRANQIRLFFSSIAYTLLEALRRLGLAGTSMAQAQ
                     CQTIRLRLLKIGALVQVTVRKVWVRLASSCPSADVFRLAFGNLDRMRPVVLRC"
BASE COUNT          666 a         1094 c         1035 g          730 t
ORIGIN      
        1 agtcacgccg ccgtcggtgc gcttcctcgg gccaaaggtc tacttgggcg cgatcgtcat
       61 cctgatcagt gctatgcggc aaggaccgtc accacgccgg gtgcgtgagc tttccacacg
      121 cttcggcgcc gaccgccgga ccatcgcccg ctggcaggtc ttctggcgag aatgctttcc
      181 ccacacgcca ttctggaagg tcgcacgcgc ccgcctggtg ccggtcgtca agatcgtcag
      241 tctgccatac tcgctagtag acgcctttct ttgccgccat ccgccttgca ggggctggac
      301 gctccttttg cggtttctct caccgatcac ggtccccggt ggcctgcgaa tcgaggtcgc
      361 gcgctgatcg ctttcaaccc gcagaagatg ctcgtcgtcg attttgatca gccggtttat
      421 acaggttgtc acagtccctt caccctactt gtgaggagat gtgatgactc aaccagactc
      481 ctgttcaacc ggcgccttgt gggcgcggtt tcggttctcc gtggtcggcc cgctcttgag
      541 ctctccccct gcgcgcggaa cgctccggac agcgatccgt tcgcttgccg agaagacctg
      601 ggcacatccg gtgaccgggc gcgacgtccg gttcacggct gtcagcatcg agcgctggta
      661 ctacacggca cggcgccagc gcgacgaccc ggttggtgcc ttgcgccgcg ccgtacgcaa
      721 agactccggc aagatctcgc tgacggcagc cctcgctgag cgactgcatc ttcagtaccg
      781 cgagcatccg cactggagct ttcaactgca ctacgacaac ctcgccgcct tggtgaaagc
      841 cgatccgacg cttgggaagc tacgctctta ttccacggtc aagcgctaca tgcgggccca
      901 cggcctggtg aagaggccca cggttcagtt gcaccaaggc cctggcgaag cccgcgccga
      961 acgaaggcgg cagacgcacg aggtccgcag ctacgaagcc cagtacgtcg gagctttgtg
     1021 gcatctcgat tttcaccacg ggtcgctcaa ggtgctgact cccggcgggc aatggcaacg
     1081 ccccatcgcg ctggccatcc tcgatgacca ctcgcgactg tgctgccacg tccagtggta
     1141 tctctcggag accgccgagg atctggtcca cgggttctcc caggcgatcc agaaacgggg
     1201 tctgccccgc gctctcctga ctgataacgg ctccgcgatg gtggccgagg aatttacaga
     1261 gggtctgctg cgcctgggaa tcgttcatga gcggacgctg ccgtacagcc cctatcagaa
     1321 cggcaaacaa gaggcgttct gggggacgct cgaggggcgg ctcatgaaga tgcttgacgg
     1381 cgtggccgag ttgacgctcg atctcttgaa cacggcaacc caggcgtggg cggagatcga
     1441 atacaaccac gccgcgcacc gcgagacttc ctgctcgccg gtcgagcgtg ttggccgggc
     1501 gcccgacgtg ctccgcttaa cctgcatttc tcacccatat atttcggagt gatctgcagg
     1561 cggctggttc ggcttctttc ctacctattt tctcatcgcg gattgttgtg ccctacttcg
     1621 ttacggtcct ggaggaccga gttcgacgca gcgggactgt cgagggccgt ttcgaccagt
     1681 ttttgacaga atcggcgcgc actgcgatcg tcgagttgcc ctcggaatgc cccctccgag
     1741 tcgcgtatcg gctatcaggt ctgccttagc atcgaagaac caccggtcgc atcctatcca
     1801 agttaccgaa cgccaggcga aagacatccg ccgaggggca actgctggcc agccgcaccc
     1861 agaccttcct caccgtcact tgcaccaagg caccgatttt cagtaaccga agcctgatcg
     1921 tctggcattg tgcttgggcc atcgaggtcc cagccaagcc cagtcgccgc agtgcttcca
     1981 gcaaggtgta agcgatcgac gagaaaaaca accgaatttg attggcccgc atggttccgg
     2041 cgctagtccg atccgcaaac aagtggagct gctgctcttt gatccggttc tccatctcgc
     2101 cgcgactgca gtaatcccgt tcgtaaagtt cctgggctgg atactgctct cgatccagcg
     2161 acgtcacgac aaaccgaggg ttggctcctt tgtccaaatg ctcggccttg gccaccaccc
     2221 gccgctcgcg gctccaactg ttccgagtct catatcgcag ctcggcgaac acgcgggtcg
     2281 gttgcttggt cgtctcgtac tccactcggg cttgctcaga ttctccggcg atcattgtca
     2341 ggagccgcga gtttttggcc agcccgaaga tgtaatcgat actgctggtt tcacaccacg
     2401 ccatgatctg gtcccggcaa aatccactat ccccgcgaac gacgatcttg acgtggggcc
     2461 aggactgccg aattcgcgtg acgatccgtt ggaggtactt caacgctcca gcgctggcgt
     2521 cgatgtcgga cggccggagc cgggcacaca acaggtgatc gccacagaag atgtacagcg
     2581 gcaggaagca gtagtgcttg tagtaaccat ggaagaagcg gcccaattga tggccgtgga
     2641 ccggatcatc ggtggcgtcg aggtcgagca caatccggtc cggcggtacg gggtaggctt
     2701 ggaggaacaa ctcaacgaaa agatcttcga cggcccgtgt attacaagtg atcttcttat
     2761 accggctatc ctcatcggcc ccgacggggg tcagttcgag ccggttgagc gtgctcttgc
     2821 ccgccagggc cttgcccttg tcacgcttcc ggagtcgtga ttggcctgtt ggatctttct
     2881 tgcccacgat ggttgccagc aaggggtcgt gccgcaggcc atcatggtca ttgaggtcct
     2941 cgtagcccag agccagagcg tagactctct gagcgacgag atgcacgagc gagtgctcga
     3001 tgagctcggg atcacgatga tcgatgaagc actcggcgaa ctgccggatg atcgctgtga
     3061 gctcctcggt cttgcgcaga agcaggctac cgccatcgga ggaaatatcg ccgccatcaa
     3121 aagcggcgac gacctggcga gagccgagaa ccgggaaatc caggtaggtt acgttggcac
     3181 cgacgagcgg gatctccacc cggggtggcc gacgatcgat gcgccggcga cgccagggag
     3241 tcgctttacg gtccgctttg gatttacact ctgtcatagt cgggtgtcct ttcaaagtgg
     3301 cgtatatcgt tgcaccaaat atatatacgt ctagaaaggg cacccgacga catatactgt
     3361 gagaaattca ggttagcctc gacaagatcg tccaatgcca cctgccgatg catcaactcg
     3421 tgatcttcgt cgtggcggaa ccgtttgttg accactgata ccgagaatcc atcggatggg
     3481 taatggtggc ggacttcagt ccgccccaga tcgacgttgg tcgac
//
LOCUS       DATAQL010000572         3991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2653989, whole genome shotgun sequence.
ACCESSION   DATAQL010000572 DATAQL010000000
VERSION     DATAQL010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653989"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VHS97_09915"
     CDS             complement(<1..328)
                     /locus_tag="VHS97_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448558.1"
                     /translation="MNQTTSSLDPWKLVWGQPYIDSRTLALAIEQDLERDTCPDFRTR
                     LLVRDAAVALRSYWGAKKFSRWLAISLVGQRVRSILDEDLGQTGFPAIRRRLVESIDA
                     TRLSRIF"
     gene            650..1978
                     /locus_tag="VHS97_09920"
     CDS             650..1978
                     /locus_tag="VHS97_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3448559.1"
                     /translation="MHKRLSFVLVATMAVAAAVGAAPEQARSTQRLPEGFMPLFNGQD
                     FAGWHGLATMDPRKFDALDADAKAKALADGAADLKKHWRVEDGVIINDGQGAYLTSDK
                     DYGDIELLVDFKIGPKGDSGVYLRGTPQVQIWDFSEPSYARNAANKGSGGLWNNTAGA
                     NGKDPLTRADNPIGEWNTLRIIQVGARTTIYLNDKLVVNHAILENYWKRSLPIPAKGP
                     IQLQTHDHEIKWRNIGIREISPDEANQILAKHGGDGFHSIFNGQHLADWAGPIEHYEV
                     KDGILKCKPHQGGTIYYNKELTDFSARLEFKLPPGGNNGLAIRYPGHGDTAYAGMCEL
                     QILDDSHPRYAKSLDPRQAHGSAYGMAAAHKGYVRPAGQWNFEEVTVKGSKIKVELNG
                     TVILDTDLSKVKDFMANSAHPGKDRTSGFFGFAGHNDPVEFRNVSIKTLD"
     gene            complement(2005..2175)
                     /locus_tag="VHS97_09925"
     CDS             complement(2005..2175)
                     /locus_tag="VHS97_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448560.1"
                     /translation="MSAGQAILGGTAPFFATGLIELSGNKLLPAVYLVICSVMAGIAS
                     LFITEQRGHAPS"
     gene            complement(2179..3105)
                     /locus_tag="VHS97_09930"
     CDS             complement(2179..3105)
                     /locus_tag="VHS97_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3448561.1"
                     /translation="MKIVNNVLQTRTGRKLEVREYGDDAGHTVFFFHGLIGSHYQAAY
                     IADQAREHGLRIIAPNRPGVGASEFVERKSALEAVDDIEDVAAALGLFEFSVIGISGG
                     TPYALATLLRLGSRIRTVTLISGMGPMRLPGALEGMDRRRRLILAVGSRYPQVARRRL
                     RTELVRFRADPDRFLRRLIATWSVSDRQLFKRKEVYDLFMKDLHQVFTEGRGPESLAQ
                     ELTLYRNYGFSLKDLPAYRRITLWQGLDDNIVPPAMAWQLIQTLPNREAHFVPGGHFV
                     AVDIAGQIITRLMQLLDGPADLPAPAPVGREI"
     gene            complement(3105..>3991)
                     /locus_tag="VHS97_09935"
     CDS             complement(3105..>3991)
                     /locus_tag="VHS97_09935"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3448562.1"
                     /translation="SNTGYMVLGSVVAKVSGKPLGQFIKERILDPLSMAHSAFEPGEG
                     MKDNTRGYTSFALGPLEPAEPEASGWLHAAGGLWASAPDLARWDVALMEGRILKPESF
                     RFMTTPRTLKNGQTTGYGCGLNVRQIDRETVLTHGGAVSGFLSFNALVPRTRSAVIIL
                     TNTEHLPVDSLYSTILRLLLDDHKKEGSPHVPKVNGPSPKEAALDFLHQMQAGKVDRH
                     KLGEEFGLFVTDERLQAAAPRLKALGEPEKVEVMSVSERGGMEVASIHFKFKTAELGG
                     LLYRTPDGKIQQLLFRKQ"
BASE COUNT          859 a         1253 c         1134 g          745 t
ORIGIN      
        1 caaagatccg gctgagtcga gtcgcgtcga tgctttccac gagtcgtctc cggatcgcgg
       61 ggaatcccgt ctgccccagg tcttcatcca aaatcgaccg gacccgttga cccacaaggc
      121 taatcgccag ccaccgcgag aacttcttgg ccccccagta cgatcgcagc gccacggccg
      181 cgtcgcggac caagaggcgc gtccggaagt cgggacacgt atcgcgctcc agatcctgct
      241 cgatggccaa tgccagcgtc cggctgtcga tgtagggctg tccccacacc agcttccaag
      301 ggtcgagaga ggaagttgtc tggttcatcg aacgaactgc gttcatgcgc ccgggatacg
      361 atggtcggcc caataggaat agcggggccg gcacggtgat cgcgtccgaa ttcttacctt
      421 acttccgcca gtctactcct gagcattgtg ccgaatcaaa ttgaacgtcg tcattccgct
      481 cgtccgctca cacgctacgt gactgcaatc tccggtcccg cccaaaccga tccgagtagt
      541 tgatctcgat cgccggtcct ccgatgatag gtggtcggcg gccgcggtgg ctctacaccc
      601 ggcccgatgc ttttgagcca gatgatcaaa tgtagaagag attgctaaca tgcacaaacg
      661 tctttcgttc gttctagtcg cgactatggc ggtggccgcg gccgttgggg ctgcgcccga
      721 acaagcgcga tcgacccagc gacttcccga aggcttcatg ccgctcttca acggccagga
      781 ttttgccggc tggcacggct tggcaacgat ggatccgcgc aagttcgacg cgctcgatgc
      841 tgatgcgaaa gccaaggcgc ttgcagatgg cgccgcagat ctgaagaaac actggcgggt
      901 cgaggacggc gtgattatta acgacggcca aggcgcttac ctcacctccg acaaagacta
      961 cggcgacatc gagctcctgg ttgacttcaa gatcggtccc aagggagaca gcggggtgta
     1021 tctccgcggc acgccgcaag tccagatctg ggactttagc gagccaagct atgcccgcaa
     1081 cgcggccaat aaaggctcgg gcggtctatg gaacaacacc gcgggcgcaa acggcaaaga
     1141 cccgctcacc cgcgccgaca atccaattgg cgaatggaac accttacgca ttatccaggt
     1201 cggtgcgcgc acgaccattt acttgaacga caaactcgtc gtcaaccacg ccatcctcga
     1261 gaattactgg aagcgctcgc tgccgattcc ggccaaggga ccaatccagc ttcaaactca
     1321 tgatcacgag atcaagtggc gcaacatcgg catccgagag ataagtcccg acgaagccaa
     1381 tcaaatcctg gcgaagcacg gcggcgatgg atttcactcg atctttaacg gccagcacct
     1441 cgctgactgg gccggtccga tcgaacatta cgaggtcaag gacggcatct tgaagtgcaa
     1501 gccgcaccaa gggggaacga tctactacaa caaggagctg actgacttct ccgccagact
     1561 tgagttcaaa cttcccccag gcggcaataa cggactggcg atccgctacc ccggccacgg
     1621 cgacaccgcc tacgcgggaa tgtgcgagct ccagatcctc gatgactctc atcccaggta
     1681 cgccaaatcc ctcgatcccc gccaggcgca cggctccgcc tacggcatgg cagcggccca
     1741 caaaggctac gtgaggccgg ccggccagtg gaacttcgag gaagtcaccg tcaagggctc
     1801 caagatcaag gtcgagctca atgggaccgt catcctcgac accgacctga gcaaggtcaa
     1861 ggacttcatg gccaattcag cccacccggg caaggaccgc acatcgggat ttttcggttt
     1921 tgcgggccat aacgacccgg tcgagttccg taacgtgtcg atcaagaccc ttgactgaaa
     1981 ccgccggcga ctgaaatcct tagttcagct aggcgcgtgg ccccgctgtt ccgtgatgaa
     2041 caggctggcg atgcctgcca tcacggaaca tatgacgaga taaacagccg gcaacagctt
     2101 gttgcccgat agctcgatca gccccgtggc gaagaaggga gcagtgccgc ccaggattgc
     2161 ctggccggcg gacacttact agatctcacg gcccacgggc gccggcgccg gcaagtccgc
     2221 cggtccgtcg agcaattgca tgagccgcgt gatgatctga ccggcaatat ccaccgccac
     2281 gaagtggccg cccggaacaa aatgggcctc acggttggga agggtctgga tcaactgcca
     2341 ggccatcgcg ggcggcacga tgttgtcgtc aagcccctgc cacaaagtaa tgcgccggta
     2401 tgccggcaga tctttaagtg aaaatccgta attccggtaa agggtcagtt cttgcgccag
     2461 gctctctggc cctcgacctt ccgtgaagac ctgatggaga tctttcatga acaagtcata
     2521 gacttccttc cgcttgaata gctgacggtc cgagaccgac catgtcgcga tcagtcgccg
     2581 caagaatcga tcagggtccg ctcgaaaacg aaccagctct gttcgtaagc gccgcctcgc
     2641 cacttgagga taccgggagc cgacggcgag tatcaggcgc cggcggcggt ccatcccttc
     2701 gagcgcaccc ggcagccgca tgggccccat gccgctgatc aatgtcaccg tgcgtatccg
     2761 ggagccaaga cgaagcagag tcgccagggc atagggagta ccgccggata tgccgattac
     2821 gctgaactcg aagagaccca aggccgcagc gacatcctcg atgtcgtcaa ccgcttccag
     2881 cgcgctctta cgttcgacaa actccgacgc tcccacgccg ggccggttgg gagcgatgat
     2941 ccgcagcccg tgctcacgag cttgatcggc gatgtaggcc gcctggtagt gagagccgat
     3001 caggccgtgg aaaaaaaaca ccgtatggcc cgcgtcgtcc ccatactctc tcacctccaa
     3061 cttccggccg gttcgcgttt gaagaacgtt gttgactatc ttcatcactg ttttcggaac
     3121 aagagttgtt gaattttccc gtcgggcgtc cggtacaaga gaccgcccag ttcggccgtt
     3181 ttgaatttga aatggatcga ggcgacctcc atgccaccgc gctcggaaac gctcattacc
     3241 tcgaccttct ccggctcacc aagtgccttc aggcgtggtg ccgccgcctg aaggcgttcg
     3301 tcggtaacaa acagtccaaa ttcctcgcca agcttgtggc gatcgacttt gcccgcctgc
     3361 atctgatgga gaaagtcgag ggcggcttcc ttgggtgacg gcccgttcac cttgggcacg
     3421 tggggacttc cctctttctt atggtcatcg agcaagagcc gcagaatcgt cgaatagagc
     3481 gaatccacgg gcagatgttc ggtgttggtc aagatgatca cagccgatcg agtccgtgga
     3541 acgagcgcat tgaacgacaa gaagccgctg actgcgcccc cgtgggtcaa gaccgtctcg
     3601 cgatcgatct gccggacatt caggccgcag ccatagcccg tagtctgacc gtttttgagg
     3661 gtgcgcggag tggtcatgaa gcggaacgac tctggcttca agatgcgacc ttccatcaag
     3721 gccacgtccc agcgcgccag gtccggggcc gatgcccaga ggcccccagc cgcatgcagc
     3781 cagccgctgg cctcgggctc cgcgggctcg agcgggccta aggcaaacga cgtgtagccc
     3841 ctcgtgttgt ccttcattcc ctcacccggt tcgaaggccg aatgcgccat cgagagcggg
     3901 tcgagaattc gctccttgat aaactggccc agcggcttgc cgctgacctt ggcgactaca
     3961 cttcccaaaa ccatgtaacc agtattgctg t
//
LOCUS       DATAQL010000573         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_819797, whole genome shotgun sequence.
ACCESSION   DATAQL010000573 DATAQL010000000
VERSION     DATAQL010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819797"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VHS97_09940"
     CDS             complement(<1..905)
                     /locus_tag="VHS97_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448563.1"
                     /translation="MVIVLIWWMTQSADRSHLDGKSPAMDSQLNSIADRPAGLHDADC
                     LMGGGEAGAHMRAHDWSATSVGPVSQWPMSLQAAVGICLNSRVPSAIWWGREHMTAFY
                     NDPYQIILRRADGLTSLGRPCRDRWAGNEEIFRPVIDRVFSSGAGECLEDQLVVVDRT
                     LREEEAYFTFSFSPIHVPPGNVGGIFCSGVETTSRVIAERRLRALRDLGASAAAFQSI
                     AAVCDSAARIMGENSHDTCFCLIYLLEGDGNRARLMAAFGVDPGSDLAPRLIEFAGKS
                     ENWPPADICRGIRTELMWKLGDKAG"
     gene            1211..1912
                     /locus_tag="VHS97_09945"
     CDS             1211..1912
                     /locus_tag="VHS97_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3448564.1"
                     /translation="MDCERNCLSDHIRKALAQRIIDGSLKPGERLVELKIAREFNSSQ
                     TPVREALRELESLRLIESLPYRGTRVREVSEREMAEAYAVRAALERLAAESAVTVLKG
                     KAVALRQSVANLLAAARAGDREGYASHDLDLHRAIVSASGNRTLLAIWESLAFETKTR
                     LSLARRQLDLVTLAAWHETVIDALEAGDGETAGRLLKDHAESFMSPLPNTGLPEIVAD
                     PVASSGEETTKKAFS"
     gene            1909..2121
                     /locus_tag="VHS97_09950"
     CDS             1909..2121
                     /locus_tag="VHS97_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448565.1"
                     /translation="MSKLVLYEEAATRRRMMPPSTHHRIGGTPQMVKVLDEFRRYAAG
                     HKGVWFARKDQIARVALETAVTVREA"
BASE COUNT          488 a          742 c          708 g          454 t
ORIGIN      
        1 tggccagcct tatcgcccag tttccacatc agctcggtgc ggattccacg gcatatatcg
       61 gcggggggcc agttctcgct cttgccggcg aattcgatca gccgcggcgc cagatctgag
      121 cctgggtcga cgccaaaagc ggccatcagt ctcgcacgat tgccgtcacc ctcgaggaga
      181 tagatcaagc agaagcacgt atcgtgggaa ttctccccca tgattcttgc tgccgaatcg
      241 cacaccgcgg cgatcgattg aaaagcggcc gcgcttgctc ccaggtcacg tagcgccctg
      301 agcctccgct cggcgatgac gcggctggtc gtctcaactc cggagcaaaa gattccgccg
      361 acattccccg gcgggacgtg aatcgggctg aacgagaacg taaagtaagc ctcttcctcc
      421 cggagagttc ggtcgaccac caccagttga tcttcgagac attcacccgc gccgctgctg
      481 aatacgcgat cgatgaccgg tcggaagatt tcttcgttgc cagcccaacg atcacggcac
      541 gggcgcccca aggaagtcag gccgtcggct cgtcgtaaaa taatttggta gggatcgtta
      601 taaaacgccg tcatgtgctc tcggccccac cagatcgccg agggcacacg cgaattgaga
      661 caaatgccaa ccgcggcttg gaggctcatt ggccattgac ttacggggcc gaccgatgtc
      721 gccgaccaat catgcgccct catatgcgcg cccgcttcac caccccccat gaggcaatcg
      781 gcgtcatgca gaccagcggg ccgatctgcg atcgaattga gctgactgtc catcgccggc
      841 gacttcccgt cgaggtggct ccgatcggcc gattgcgtca tccaccagat cagaacgatt
      901 accatgttct cggatcggcc gtgactgcgc actcggcgat aacgccaaag tgaacagctt
      961 cgcacgacgt ggggccaatc agtgcgatcg gccgtcaact gaagctaccg ccgcagatct
     1021 gattcccgtt cattatgggc tgcctgccat cctttttccc agacagcgac gtgctcgagc
     1081 acaacctttt gcgttttcta tgcggtacag atgaccggta tatcgtgacc cccgtcaaaa
     1141 tacgaccctt gtccggagat tatcgataat atagaatctt tctgcgttgg aacctcattg
     1201 gggacaatcg atggactgcg aacgcaactg cctgagcgac cacattcgca aggctctggc
     1261 ccagcgaatc atcgatggtt cgctcaagcc gggtgagcgg ctggtggagc tcaagattgc
     1321 gcgcgagttc aattcgagcc aaactccagt ccgcgaggcg ttgcgcgagc tggagtcgct
     1381 tcggctgatc gaatcgctgc cctaccgtgg cacgcgcgtc cgcgaggtca gtgagcggga
     1441 aatggccgag gcatatgccg tccgggccgc gctggagcga ttggccgccg agtcagccgt
     1501 aactgtcctt aagggaaaag cggtcgcgtt gcgccagtcg gtcgccaacc tccttgccgc
     1561 cgcacgcgcc ggcgatcgcg aggggtacgc cagccacgac ctggacttgc atcgcgcgat
     1621 cgtctcggct tcaggcaaca gaactctgct tgcgatctgg gagtcgctcg ccttcgagac
     1681 gaagacaagg ctgtcactgg cacgacgtca gcttgacctc gtgacgctgg cggcctggca
     1741 tgaaaccgtc atcgatgcgc tcgaggccgg tgatggcgag acggctggcc ggctactcaa
     1801 ggaccacgcg gagtcgttca tgtcccccct cccaaatacg ggcctacctg agatcgttgc
     1861 tgaccctgtg gcgtcatcgg gtgaggaaac gacgaaaaag gcattttcgt gagtaagctg
     1921 gtcctttacg aggaagcggc gacgcgccga cgcatgatgc caccgagcac gcaccaccgg
     1981 atcggcggca cgccccagat ggtcaaggtg ctcgacgaat tccgcaggta cgcggccggg
     2041 cacaaaggcg tctggttcgc gcgcaaggat cagatcgccc gcgttgcctt ggaaaccgcc
     2101 gtgacggttc gcgaggcttg agaacaaact ccgttcggtc acgttcactg aattcttcgg
     2161 aaagctgcgc gcgagcaaga tcattccgcc ccgctcgtga gggcgacgcg ccccgcacgg
     2221 cggcttgtca gcgtcgggaa cgatcgaata cactttctag tgagagggcg gcagtccgag
     2281 cacggttgcc gctcggtcgg aataaccgac caaagacctg ccgcggatcg atcgcttcga
     2341 ttcggtcaaa cgatgatgaa taatgagggc actcgctgat ggctcgccgg ac
//
LOCUS       DATAQL010000574         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2959252, whole genome shotgun sequence.
ACCESSION   DATAQL010000574 DATAQL010000000
VERSION     DATAQL010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959252"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..353
                     /locus_tag="VHS97_09955"
     CDS             135..353
                     /locus_tag="VHS97_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448566.1"
                     /translation="MAAKTATNTKAKSSPAKVKVETRWGAVATLKSTDVVSVVLDVPT
                     AKTLLHALAVAIGGGGNGKGKGKGKGGK"
     gene            385..2328
                     /locus_tag="VHS97_09960"
     CDS             385..2328
                     /locus_tag="VHS97_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448567.1"
                     /translation="MTDRRLPSPFIRILVEEFGDEELARELFAEFLGQLRFARAFALR
                     LVDVAGGKRRWKRTSWRLRCAAALMLESQIWATPASENGELELILRSIAGSAPGGLRD
                     PSGRSILAESHGTSVPETFVAELRGRMARLERIHRRISDPARKPEALRDFLDISRQPC
                     RLTLARYLFDPRATARQVLARVRTSHGLALPSGSPMVEAEAQSLISQWPEQEQRFLRE
                     LLDSAEIFWADESTGSRLNDMVEYPLGTVALVVKPPGSSLEIEIKRAGRPGNHPLSVV
                     FERSGQAVPPSHRLDGGSMKGPAKAEAAAAARFGRLYQLVHHRRAPISHTLTLRSIYE
                     VPTAGCPAPLLDYFTDPEIFGNGFEEMRRDMERSIRSFCNEWTVERLGTPGELGQTVD
                     FLAQVSPTQAVLGGTSSFRLEQLARYLADEGPDLYFRAGLGVTPTKDDARRLAETLSD
                     EILGKFDPVEIVYRRQSEYLDAIFSSPGNRRRADEVFLELSAEAGRFWGTLVALKGYS
                     NGESLVGRNVGLKSVWERGDWRVRIIFLDHDQLSVPSDGFSPPDVLRGWRRDAAHILG
                     EPGQGRKCVSDWLAIIYRVDSAVAKRGRKELLRAAVDAARLTRARLANDPAVRAYFQP
                     DCLMDASDWEAAAAAFVRARRHG"
     gene            2332..2475
                     /locus_tag="VHS97_09965"
     CDS             2332..2475
                     /locus_tag="VHS97_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448568.1"
                     /translation="MKAAIAAGRKLLANRGRPPDFVTRSMDAVEQFAELLAEHGPLLV
                     AET"
     gene            complement(2600..3199)
                     /gene="def"
                     /locus_tag="VHS97_09970"
     CDS             complement(2600..3199)
                     /gene="def"
                     /locus_tag="VHS97_09970"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3448569.1"
                     /translation="MLEIVNFPHPALRYVSRSVTEIDDGLRATVRAMFELMYAAKGIG
                     LAANQVALPFRFFVLNVTADRERPDQEHVFINPEIVKRHSSVEDEEGCLSLPGLYAKV
                     RRARKIRVRAYDLEGNLVEHEADELFSRAVQHEMDHLEGKLFIDYLEPLARRAIDDKL
                     RAFELEYFKNQAGGTIPADAAIAARLHAMARPNLNGLGS"
     gene            complement(3363..>3678)
                     /locus_tag="VHS97_09975"
     CDS             complement(3363..>3678)
                     /locus_tag="VHS97_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448570.1"
                     /translation="SRISPLVKALSQLETPRFVQYDGEIPPVTGLLRPRLTVEVELGS
                     PAVTRVIRIGYPTNDGYVFAAEGTSSSGPVFLLTNVAWDALIASGERFNPLPANVFAP
                     AP"
BASE COUNT          694 a         1152 c         1155 g          677 t
ORIGIN      
        1 gcgtatttcg accgcgcgat ctcttcctct ttgagcgccg acggcagccg cttgacggta
       61 gcttccggtc ggacgtttga ttcgtcgtgt gtcagagcag agtcccattc tcccctccag
      121 taaaggagct ctagatggcc gcaaaaaccg caaccaatac aaaggcgaaa tccagccctg
      181 ccaaagtcaa ggtcgaaact aggtggggtg ccgtcgcgac tttgaagtcg actgatgtcg
      241 tcagcgtggt tctcgatgtt cccaccgcga agacgctgtt acacgcgctc gccgtcgcga
      301 tcggtggtgg cggaaacggg aagggcaaag gcaaaggcaa aggagggaaa tagagaaatg
      361 cccgctcccg catgagggcg attcgtgacc gatcgtagat tgccgtctcc gttcatccga
      421 attctggtcg aagaattcgg ggacgaagag ctcgcgcgcg agttgttcgc cgaattcctc
      481 ggccaattga gattcgctcg tgcgttcgcc ctgcgcctgg tggacgtggc cggcgggaag
      541 cgccggtgga aacggacgag ctggcgactg cgctgcgcgg cggcgctcat gctcgagtca
      601 cagatatggg ccacgcccgc gtccgagaac ggcgagctcg agctcatcct ccggtctatc
      661 gctggaagcg ccccgggcgg actgcgcgat ccgtccgggc ggtccatcct tgccgagagt
      721 catggcacca gcgttcccga gacgtttgtc gccgagctcc gcgggcgcat ggcgcggctg
      781 gagcggatac accggaggat cagcgatccg gcgcggaagc ctgaggcgct gcgcgatttt
      841 ctcgacatct cacgccagcc gtgccggctc accctggcac gctatctctt cgacccgcgc
      901 gcgacagccc ggcaggttct cgcccgcgtc cggacgtcgc acggattggc gctgccgtcg
      961 ggttccccga tggtcgaggc tgaggcgcag tcgctcattt cccaatggcc cgaacaggaa
     1021 caacgattcc tgcgcgagct gctcgactcg gcggaaatct tctgggccga tgagtcaacc
     1081 ggcagccggc tgaacgacat ggtggaatac ccgctcggca cggtcgccct cgtggtcaag
     1141 ccgccgggaa gcagtctgga gatcgaaatc aagcgcgcgg gccggcccgg caatcacccg
     1201 ctttcggtcg tgttcgagcg gagtggccag gccgtgccac catcgcaccg gctggacggc
     1261 ggatcgatga agggccccgc caaggccgaa gcggccgcgg ccgcgcgatt cgggcggctt
     1321 taccagctcg tacatcaccg ccgagccccg atttcacaca cgctcacact gcgatcgatc
     1381 tatgaggtgc ccacagccgg ctgccccgcc ccgctcctcg attacttcac cgaccccgag
     1441 atctttggga acggattcga ggagatgcgc cgagacatgg agcggtcaat ccgctcgttc
     1501 tgcaacgagt ggacggtcga gcgcctgggg acgccgggcg agctcggcca gaccgtcgac
     1561 ttcctggccc aggtgtcacc tacccaggcc gtgctgggtg gcactagctc gtttcgtctc
     1621 gaacaacttg cgcgatacct cgccgatgaa gggccggatc tttacttccg agccggactc
     1681 ggcgttacac ccacaaagga cgacgcacgt cgccttgccg agactttgtc ggacgagata
     1741 ctcggcaagt tcgatccggt cgaaatcgtc tatcgccgcc agagcgaata cctcgacgcc
     1801 attttctcga gtccaggtaa ccgccgaagg gctgacgagg tgtttcttga gttgagtgct
     1861 gaggctggtc gcttttgggg gaccttggtc gcactcaagg gttactcgaa cggcgaatcg
     1921 ctggtcggtc gcaacgttgg gctcaagtca gtctgggaac gtggcgattg gcgagttcgc
     1981 atcatctttt tggaccatga ccagctcagt gtccccagcg acggtttttc gccccccgac
     2041 gtgcttcgcg gctggcgccg cgacgcggcg catattctgg gcgagcccgg ccaagggcgg
     2101 aaatgcgtca gcgactggct ggcaatcatt tatcgcgtcg actctgcggt cgcaaagcga
     2161 ggtcggaaag agctgcttcg cgcggccgtt gacgccgccc gactgacgcg tgcccgcctg
     2221 gcgaacgatc ccgcagtccg cgcgtacttt caaccggatt gcctcatgga cgcatccgat
     2281 tgggaggccg ccgcagccgc attcgttcgc gcccgccgac acggctgaag cgtgaaggcc
     2341 gcaatcgccg ctggtcgcaa gctgctggcg aatcgtggac gcccaccaga ctttgtcacc
     2401 agatcgatgg acgccgtcga acaattcgcc gagctgctcg cagagcacgg cccgctgctc
     2461 gtcgccgaga cgtaagagtc tccctgccga gcgcctgagt ctgaagcggg aaattttttc
     2521 gagccgaatc ttaaatgtgt tcaaccgcga aagccaagcg tccgctctgc cagcggcaaa
     2581 gtccctcccg gatctgaact cacgagccaa gaccgttcaa gttcggccgc gccatcgcat
     2641 ggagtcgagc ggctatagca gcatcagccg ggattgtgcc gccggcctgg tttttgaagt
     2701 attcaagctc gaaggctcgc agcttgtcgt cgatcgcgcg cctggccaac ggttcgagat
     2761 aatcgatgaa aagcttgccc tcgaggtggt ccatttcgtg ctggacggcc cggctgaaca
     2821 gctcgtcggc ttcgtgttcg acgaggttgc cctccaggtc ataagcccgg acccggatct
     2881 tcctggccct acgaaccttg gcatagagcc cgggcaggct caggcagccc tcctcgtcct
     2941 cgaccgacga gtgccgcttg acgatctcgg gattgatgaa cacgtgctcc tgatctggcc
     3001 gttctcgatc ggcggtcacg ttcaagacga aaaaccgaaa tggaagcgca acctgattgg
     3061 ccgccaggcc aatgcccttg gccgcgtaca tcagttcgaa cattgcacgg accgtagcgc
     3121 gcaacccgtc gtcaatctcg gtcacggacc gagagacata gcggagtgcc ggatggggga
     3181 agttgacaat ctcaagcacg cggaacgctc cctgggcgca atcaatcaga ttaatcttag
     3241 cttaacggcg ctgacatgag cttaccatgt cgatcaccgc gacgacggcc gcagcaggtg
     3301 gaccggtcgg cgcatctcgg aagatagcgg ctctcgaccg gtgcggggta caaatcggtc
     3361 gttcaaggcg caggtgcgaa gacgttggcg ggcagggggt tgaatcgctc gccagaggcg
     3421 atcaatgcgt cccaggccac gttcgtcagg aggaagaccg ggcccgagct ggacgttcct
     3481 tcggccgcaa acacatagcc gtcgttcgtg ggataaccga tccggatcac gcgcgtcacc
     3541 gccggcgaac ctagctcgac ctcgaccgtc agccgtggac ggagcaaacc cgtcacgggc
     3601 ggaatctcgc cgtcatactg cacaaaacgg ggcgtttcga gctgcgagag tgctttcacg
     3661 agtgggctga tccgcgat
//
LOCUS       DATAQL010000575         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1432841, whole genome shotgun sequence.
ACCESSION   DATAQL010000575 DATAQL010000000
VERSION     DATAQL010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432841"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1252
                     /locus_tag="VHS97_09980"
     CDS             257..1252
                     /locus_tag="VHS97_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX3448571.1"
                     /translation="MLTLYHSRVGRGERPANLAAESLPKNVAWIDLLNPEPDEVAFVK
                     RTTGLDVPSVEDLSEIESSSRLRSRNGAIYLSAPLVYRAVSDQPLTTPVGFVLTRERL
                     ITVRFEELSLFNAFASRDLAAESDPLSSSAIFAGLMDAIADRLADILENIAAELDSLS
                     HRLFRTPATEPAARRPPARESADLRIILRRVGHSGDLASKIRDSLLGISRIVPYVLSL
                     ASDWLPPDIKSRLDTVRQDVLSLSDYDAHIVNKVQLLLDATLGLINVEQNNIIKVLTI
                     VSVVGIPPTLIASMYGMNFKGMPEYDWSWGYPYGLAMIALSAILPLVWFKWRGWI"
     gene            1310..1579
                     /locus_tag="VHS97_09985"
                     /pseudo
     CDS             1310..1579
                     /locus_tag="VHS97_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(1935..2378)
                     /locus_tag="VHS97_09990"
     CDS             complement(1935..2378)
                     /locus_tag="VHS97_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3448572.1"
                     /translation="MELPPALRLSFEDNPSAQDRKAVEEALGAYNAPFLRNPPYSYFG
                     IFVRDEVNAIRAGLIGNCYAGWLFVALLWVHADLRRIGIGSRLMAEAERHGRHFGCHS
                     AFVDTFTFQGPEFYPKLGYREYARLDYPPDHHRIFFRKNLDINVV"
BASE COUNT          541 a          808 c          820 g          512 t
ORIGIN      
        1 agccccggcc cgagatagcc ggggctcttc atactatcgc gggagtaatc cacccgttgc
       61 cacgccaccc gccgtagggt ccttgcccgc ccccataacc accccagacc tcgtagggtc
      121 gcctgggctc gcgaggcgca cgaaaataag tgccgcaccg agagccggta ccgaggaaga
      181 gccgatattt atggaacaat ttgatgaatg tgggttttcg cgagaaacca gaccatcgcc
      241 gctataagtt cggccgatgc tgacgctcta tcacagccgc gtggggcgcg gggaacggcc
      301 ggccaatctc gccgcggaga gcttgccgaa aaacgtcgcc tggatcgatc tgctcaaccc
      361 cgagccggac gaggtcgcat ttgttaagcg aaccacgggg ctcgatgtac cgagtgtcga
      421 ggacctgagc gagatcgaaa gttcaagccg gctgcgcagt cggaatggcg cgatttatct
      481 gagtgcgccg ctggtctatc gcgccgtttc cgaccagccg ctgacgacgc cggtgggctt
      541 tgtgctcacg cgcgagcggc tgatcacggt gcgcttcgaa gagctgagcc tgttcaatgc
      601 gttcgccagc cgggaccttg cagccgaaag tgacccgtta agcagctctg ccatttttgc
      661 gggtctgatg gacgcgatcg ccgatcgcct cgccgacata ctcgaaaaca tcgccgccga
      721 gcttgattca ctgtctcacc ggctgtttcg caccccagct acggaacccg ccgcacggcg
      781 cccaccggcg cgtgaaagcg ccgacctgag gatcatcttg cgccgcgtcg gccacagcgg
      841 cgatctcgcg tcgaaaatcc gcgacagcct gcttgggatc agccggatcg tgccctatgt
      901 cttgagtctt gccagcgatt ggctaccgcc ggacatcaag tcaaggctcg atacggtgcg
      961 ccaggacgtg ttgtcgctca gcgattacga cgcacatatc gtcaacaagg tccagcttct
     1021 gctcgatgcc acccttggtc tcatcaatgt cgagcaaaac aatatcatca aggtgttgac
     1081 gatcgtttcc gtggtcggca ttccaccgac cctgattgcg agcatgtatg gtatgaattt
     1141 caaaggcatg ccggaatacg actggtcctg gggctatcct tacgggcttg ccatgatcgc
     1201 cctgagcgcg atcttgccgc tggtctggtt taaatggcgc ggctggattt gagccgatgc
     1261 ctactggcga gagagttcat gacatcggag ctagagctgg gggccccagg tgaatcaatc
     1321 cgaactgatt gagaaggtcg ctcaggcaac tgagctaaac caagcggcgg tgggccaggc
     1381 ggtgaaggcc gtggtcaacg ccatcctcga tgccctcgtg gccggcgagg cggtccgtgt
     1441 ccctggcctc ggctctttcg atgtggcggc gcgacctgca cgcgaggggc ggaatccaca
     1501 aaccggaggg gccatccaaa tcgcagccag caaggcagtg cggtttcgcg caggtaaggc
     1561 cgtcaaggag gcgctcaatc cgccaactgc aaaggcggct ccccggaaga aggccgcacc
     1621 accaaagaaa tccgcagcac ggaagtaaac gtcctctcgc tgtacgagca cgggtattag
     1681 gctcgtgcgc gatcatcgct ccgtccgtcc gatacacgat ccatctgatc cggagccaaa
     1741 acgctccagc aaaagcgccc cgcgtcccgg gctggtgtca agcgggacca agagttcgaa
     1801 tccggcttgc tccagcggag agtccggtgc gaactcgagg ttgcgggttc ggcggccgac
     1861 ttccggcaag cgaactcgcc gcgttcgctc cttacgataa gctttttctc gtatccgact
     1921 tatgaccagc aaccctagac aacattgatg tcgaggttct tgcggaagaa gatccgatgg
     1981 tggtctggcg ggtaatcgag ccgggcgtat tcgcgatagc cgagcttcgg gtagaactcc
     2041 ggcccctgga acgtgaaggt atcgacaaag gcggagtggc acccgaagtg gcggccatgc
     2101 cgctcggctt ccgccatcaa gcggctcccg atcccgatcc ggcgtagatc ggcgtgcacc
     2161 cacagcaatg cgacgaacag ccagcctgcg tagcaattgc cgatcaaccc ggcccggatc
     2221 gcgttgacct cgtcacgaac gaaaatgccg aaatacgagt agggtgggtt ccgcagaaac
     2281 ggcgcgttgt acgcaccgag cgcctcctca acggccttgc ggtcctgcgc ggacggattg
     2341 tcttcgaagc tgaggcgaag cgctggtggc aattccattt tgggtctcac ggagcaagga
     2401 ttggtggact aggaatccga tctgcgctcg gtcatagggg atcattcaac atcccgcccg
     2461 ccccgggccg gttactcatt cgatgacctc atcagcgcgg gcgaggatcg attgcgggat
     2521 cgtcaggccg agcgctttgg ggtgttaaga ttgaccacca gctcgaatgt ggtcggttgc
     2581 tggactggca aatcggaggg cttttcgcca ccgagtatcc taccgacgta agtgccgatc
     2641 tgtcggtaca ctccggcgag gcttgtgccg tagctgatca g
//
LOCUS       DATAQL010000576         5561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2196464, whole genome shotgun sequence.
ACCESSION   DATAQL010000576 DATAQL010000000
VERSION     DATAQL010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196464"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VHS97_09995"
     CDS             <1..266
                     /locus_tag="VHS97_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448573.1"
                     /translation="QTVNNEIALAKSDLELALTTKGSDGQGGEEPSIRLKRAQKKKAN
                     LETHTKDKMVGELMGEVAKAVATEEAAKAAFDQLTAAVANSWW"
     gene            585..3722
                     /locus_tag="VHS97_10000"
     CDS             585..3722
                     /locus_tag="VHS97_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3448574.1"
                     /translation="MPQRPNWWHWLFAPSRPKLRQLFPATVAATALVLGVLAFCSDPV
                     SAADRPALEYNRDVRPILAENCFACHGSDSAARKADMRLDRRDDAIKAGAIAPGDTES
                     SELIARINAQDPQELMPPPVTAKKLTQDQKQILGRWIAEGAAYQPHWSLIPPKRPALP
                     VVQNEAWIRNPIDRFVLAKLEEKGLRPAPEANRRTLARRLSLDLTGMPPEPAVVEEFL
                     QDGAPDAYESLVSRLLDSPRWGEHRARYWLDSARYADTNGYHFDNFREAWAFRDWVVD
                     AFNRNLSFDRFTIEQLAGDLLPGSTLDQQIASGFNRCNSTTNEGGVIPDEYQVLYTRD
                     RTETVSQVWMGLTAGCAVCHDHKFDPISQREFYELAAFFNNTTQPTMDGNIKDTPPTV
                     FVPSEADRARSEIVAAELAAARKGIEAREPSARSDFAKWIGALDTKALESTIPTAGLR
                     LAAINVPAALAPDTSQTFESADTGDFDKDHAFSFGAWIKVPKSGLSGSVMARMDDRDN
                     YRGWDMLLEGGRIGTHLVHEWPSDALKVVSRAEVPPGVWTHVLVTYDGSARAAGVKIY
                     LNGEPQEVEAKADGLKQTIRTEVPFKLGQRHTSSRLDDLAVHSLRIYDKALSPPEAGV
                     LAGAARAAALARMPADKRPRDEVDGVFGWWRRVVDPLSREQRDKLAVLAAEDAAIKKR
                     GTFAHVMHERSEPAMAYVLHRGEYDKRRDPVKPNTPGALPPMPAELPKNRLGLARWLI
                     APEHPLTARVVVNRFWQEVFGAGLVRSAGDFGVTGETPSHPELLDWLAVEFRTSGWDV
                     KRLFRLIVESSTYRQSVVMTPENRDKDPHNRLLSRGPRFRMDGEMIRDAALGSSGLLV
                     GKIGGPSVKPYQPEGVWEAVAMPESNTHFYRPDRGDRLYRRSLYTFWKRSAPPASMEV
                     FNAPSREVCTIRRERTNTPLQALVTLNDPQFVEAAKALAQRALKQGGLSDDERIDFIA
                     TRLLSRSLRAEELAVVQTSLGRLVANYQSHPDQATELLAVGESKAETSFDTSALAAWT
                     MLTNELMNLDEFLNK"
     gene            3865..5361
                     /locus_tag="VHS97_10005"
     CDS             3865..5361
                     /locus_tag="VHS97_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3448575.1"
                     /translation="MHPMLDYVRYETRRQFLSRGSNAVSWAAMAALLGREGLVPASRA
                     DAAGADASPTPIGGRRLPGLAHFAPKAKQVIYLHMVGGPPQMDMYDYKPTMGSWFDKD
                     LPDSIRMGQRLTTMTSGQKRFPIAPSKYKFAQHGQSGMWVCELLPYTSTMVDDMCFIR
                     SMHTEAINHEPAITYMQTGNQITGRPCLGAWVSYGLGSLNDNLPTFVVLVAKPTNTEQ
                     LQAISARLWSSGYLSGEHAGVSFRSGGDPILFIQNPAGVSSEVRRDTLDGLKALNEMN
                     YQLVGDPETHTRIEQYELAFRMQSSVPELTAITNEPDSTYRLYGEEAKKPGSFAHSAL
                     LARRMVERGVRFVQIYHNNWDTHFNVGGRLPSQCRDVDQPCTALIRDLKARGLLDETL
                     VIWGGEFGRTIYSQGGLKHDNYGRDHHPRCFTMWMAGGGSRPGTIYGETDEFSYNIVK
                     DPVHIRDFHATVLQLLGFDHKRFTYRYQGLDQRLTGVEPARVIPQLMA"
     gene            5375..>5561
                     /locus_tag="VHS97_10010"
     CDS             5375..>5561
                     /locus_tag="VHS97_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448576.1"
                     /translation="MDKKGAGGWGRTGYCQRAPGALARIRGRRPMGVDPSHPLRDASD
                     GERVIPLAATPNELLKPD"
BASE COUNT         1126 a         1753 c         1675 g         1007 t
ORIGIN      
        1 agcagaccgt caacaacgag atcgctctgg caaagtctga tctcgaactc gcgttgacaa
       61 ccaagggttc cgacgggcaa ggaggcgaag aacccagcat ccggctcaag cgggcccaaa
      121 aaaagaaggc caacctggaa acccacacca aagacaagat ggtcggggag ctcatgggcg
      181 aggtggcaaa agccgtagcc accgaggagg cagcgaaggc cgcattcgac caattgaccg
      241 cagccgtcgc gaattcatgg tggtagacgc cggcattcaa gatataatga attcggccgt
      301 acactaaaga caataccagt aaattcgcag taacgtatag gtgcatatta acttataatc
      361 ccctcccccc ttgtggggga gggccagggt ggggggtgtc cccctgcaga ccgcagccaa
      421 ctgtgttttc tggtacctgc ctccccccac cccaaccctc ccccacaagg ggggagggag
      481 acggaactga acttgggcag ataagtttca tctagttcac gctatcgaca tgaacggcca
      541 aacccgccga cgcgtcgcgc actcctatcc aggagacctc ttcgatgcct caacgcccca
      601 attggtggca ctggctcttt gccccttccc gcccaaagct ccgacaatta ttccctgcca
      661 ctgtggccgc gacagcgctt gttcttggtg tcctggcttt ttgctccgat ccggtatccg
      721 cggccgatcg gccggcgctg gaatacaacc gcgacgtccg gccgatcctg gcggagaatt
      781 gcttcgcatg ccacggttca gatagcgccg cccgtaaggc cgatatgcgg ctcgaccgac
      841 gcgacgacgc catcaaggcc ggtgccattg caccgggcga taccgagagc agtgagctaa
      901 tcgcgcggat caacgcccag gatccgcagg agctcatgcc tccgccggtg accgccaaga
      961 agttgacaca agatcagaag cagatactag ggcgctggat tgccgaggga gccgcctatc
     1021 agccgcactg gtcgctcatt ccacccaagc ggccagcgct gccggtcgtt caaaacgaag
     1081 catggatccg taatccgatc gaccgcttcg tgctggccaa gctcgaagaa aaaggcctgc
     1141 gccccgcgcc cgaggcaaac cgccggacgc tggcgcgccg gctctcgctc gacctgactg
     1201 ggatgccacc cgagccggcc gttgtcgagg agtttctaca ggatggtgcg cccgacgcat
     1261 acgaatcgct ggtcagccgc ctccttgact caccccgctg gggcgagcat cgggctcgct
     1321 actggctcga ctcggcccgc tatgccgaca ccaacggcta tcacttcgac aacttccgcg
     1381 aggcatgggc gtttcgcgac tgggttgtcg acgccttcaa ccgcaacctg agtttcgacc
     1441 ggttcaccat cgaacaactg gccggcgacc tcttgccagg cagcacactc gaccagcaga
     1501 tcgcttcggg gttcaaccgc tgcaactcaa ccaccaacga agggggcgtt atccccgacg
     1561 aataccaggt cttgtacacc cgcgatcgga ccgagactgt gtcgcaggtg tggatgggcc
     1621 tcacggcggg ctgtgcggtc tgccacgacc ataagttcga cccgatcagc cagcgcgagt
     1681 tttacgaact ggcagcgttt ttcaacaaca cgacccagcc gacgatggac ggcaacatca
     1741 aggacactcc gccgaccgtt ttcgtcccca gcgaggccga tcgggcacgc tcggagatcg
     1801 tcgccgccga gctcgccgca gctcgaaaag gaatcgaagc gcgcgagccg tccgcccgtt
     1861 cggatttcgc caaatggatt ggtgcgctcg atacgaaggc actcgagtcg acgatcccca
     1921 cggctggcct gcggctcgcc gcgatcaatg ttccagccgc cctagcgccc gataccagcc
     1981 agacgttcga atcggccgat acaggggact tcgacaaaga tcacgcattc tcgttcggcg
     2041 cctggatcaa ggttcccaag tcaggattgt ccgggtcggt gatggcccgc atggacgacc
     2101 gcgacaatta ccgaggatgg gacatgttgc tcgaaggggg tcgtatcggc acgcatctcg
     2161 tccacgagtg gccgtcggac gctctcaagg tggtctcgag agccgaagtt ccgccgggcg
     2221 tctggaccca cgtgctcgtg acctacgatg gctcggcccg agccgcggga gtgaagatct
     2281 atttgaacgg agagccgcag gaggttgaag ccaaggccga tggattgaag cagacgattc
     2341 gaacggaagt gccgttcaag ttaggccagc ggcacacatc atcgcggttg gacgacttgg
     2401 ctgtacactc actgcgaatc tacgacaagg ccctctctcc ccccgaggcc ggcgttctcg
     2461 cgggagcagc tcgtgcggca gcgcttgcgc gaatgccggc cgacaagcgg ccgcgcgacg
     2521 aagtcgacgg cgtctttggc tggtggcgcc gcgtcgtcga ccctctctcg cgagagcagc
     2581 gagacaagct ggcggtcctc gcggcggaag atgcggcgat caagaagcgc gggacgttcg
     2641 cgcatgtgat gcacgagcgc agcgagccgg cgatggcgta cgtgctgcat cgaggggaat
     2701 acgataagcg tcgcgacccg gtgaaaccga atacgcccgg cgcactgccg ccgatgccgg
     2761 ccgagcttcc caaaaaccgg ctcggcctgg cgcgctggct gattgcgcct gagcacccgc
     2821 tgaccgcccg cgtcgttgtc aaccgcttct ggcaagaggt cttcggcgcg ggcttggtgc
     2881 ggtccgccgg cgacttcggc gtgaccggtg agaccccctc gcaccccgag ctcttggact
     2941 ggctggccgt cgagtttcgc acgtcgggct gggatgtaaa gcggcttttc cggttgatcg
     3001 tcgagtcatc gacataccgg cagtccgtcg tgatgactcc ggaaaaccgc gataaagacc
     3061 cgcacaatcg cctgctatcg cgtgggccgc ggtttcgcat ggacggtgag atgatccgcg
     3121 acgccgccct tggatccagc ggtctcttgg ttggtaagat cggcggaccg agcgtcaagc
     3181 cgtaccagcc cgagggggtc tgggaagcgg tggcaatgcc cgagagcaac acccattttt
     3241 accggcccga tcgtggggac aggctgtacc gccgcagtct ctacacgttc tggaagcgaa
     3301 gcgcgccccc ggcgtcgatg gaggtcttca atgcccccag ccgtgaagtc tgcacgatcc
     3361 gccgcgaacg caccaacaca ccgctgcagg cactggtgac actcaacgac cctcagttcg
     3421 tcgaggccgc caaagcactc gcgcagcgag ccctcaaaca gggtggtcta tccgacgacg
     3481 agcgcatcga ttttatcgcc acgcgcctgc tgagccgatc actacgcgcc gaggagctcg
     3541 ccgtcgtcca gacttcgctc ggcaggctcg tcgccaacta ccagtcgcac cccgaccagg
     3601 ccaccgaatt gctcgccgtc ggcgaatcaa aggctgaaac ttccttcgac acctcggccc
     3661 tcgccgcctg gaccatgctt accaacgagc tcatgaattt ggatgagttt ttgaataaat
     3721 aagtccttgg tccttagtcc ttggtccttg gccatcaagc ggcaggaaag aaccgtgaag
     3781 ttcccagagt tggatctgcc tgctttcttc atatagcgtc taaggaccaa ggaccacgga
     3841 ccaaggacta atagggagcc aaacatgcac cccatgctcg actatgtccg atatgagacg
     3901 cgccggcagt tcttgagtcg aggctctaat gctgtgagct gggccgcgat ggctgcgctt
     3961 ctgggacgtg aagggctggt ccccgcgtcc cgcgccgatg ccgcaggcgc cgacgcttcg
     4021 cctacgccca tcggcgggcg ccgtctgccc ggactggcgc atttcgcccc taaggccaag
     4081 caggttatct atctgcacat ggtcggcggg ccgccacaaa tggacatgta tgattacaag
     4141 ccgacgatgg ggagttggtt tgacaaggat ctgcccgact cgattcggat gggccagcgc
     4201 ttgacgacca tgacctcggg tcagaagcgg tttccgatcg caccgtcgaa gtacaagttc
     4261 gcgcagcacg gacaaagtgg catgtgggtc tgtgagctgt tgccgtatac gtcgacgatg
     4321 gtcgacgaca tgtgtttcat cagaagcatg cacaccgagg ccatcaacca cgagccggcg
     4381 atcacctaca tgcagacggg caatcagatc acgggccgcc cgtgtttggg agcgtgggtc
     4441 tcgtatggcc tgggatcgct caacgacaac ctacccactt ttgtcgttct ggtggcaaaa
     4501 ccgactaata ccgagcagtt gcaggccatc tcggcgcggc tgtggtcgtc gggttacctg
     4561 tccggtgagc atgcgggcgt ctcgttccgc agcggtggcg acccgatcct tttcattcaa
     4621 aacccggccg gtgtttcgtc cgaggtgcgc cgcgatacct tggacgggct caaggcactg
     4681 aatgagatga attaccagct tgtgggcgac ccggagactc acacgcgtat cgagcaatat
     4741 gaactggctt tccggatgca gtcgagtgtc cccgaactta ccgcgatcac caacgaaccg
     4801 gactcgacgt acagacttta tggcgaggag gcgaagaagc ctggttcgtt cgctcattcc
     4861 gcactcctgg cgcggcgaat ggtcgagcgt ggggtgcgat tcgtccagat ctaccacaac
     4921 aactgggaca cccacttcaa cgtgggcggc cgtttaccca gccagtgccg cgacgtcgat
     4981 cagccgtgca cggccttgat tcgggacttg aaggcccggg gccttttgga cgagaccctt
     5041 gtgatctggg ggggagagtt cggtcggacg atttactcgc aaggtggttt gaagcacgac
     5101 aactacggtc gcgatcatca cccgcgctgc ttcacgatgt ggatggccgg cggtggctcg
     5161 cggcccggca ccatctacgg cgaaaccgac gaattctcat ataatatcgt caaggatccc
     5221 gtccacatcc gcgacttcca cgccaccgtt ctgcagctct tgggtttcga ccataagcga
     5281 ttcacatacc gctatcaggg tctcgaccaa cgcctgaccg gcgtcgagcc ggcgcgcgtg
     5341 atcccgcagc tgatggcgtg acgacgctaa atgaatggac aaaaaaggtg ctggtggctg
     5401 gggtcgaact ggctattgcc agcgagcccc cggcgcactg gcccggatcc gggggcgccg
     5461 ccctatgggc gtcgacccca gccaccccct gagggatgcc tcggatggcg aacgggtgat
     5521 cccgcttgct gctacgccga atgaactgct aaagcccgac g
//
LOCUS       DATAQL010000577         5031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5402959, whole genome shotgun sequence.
ACCESSION   DATAQL010000577 DATAQL010000000
VERSION     DATAQL010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5402959"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VHS97_10015"
     CDS             complement(<1..447)
                     /locus_tag="VHS97_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448577.1"
                     /translation="MSLSRRRDNRPSLERLEGRLQLSAAIPANSIGTSVGTVTAPGQV
                     TAASVTVAPKNLTQGKSSTLFAIFVQPEPGSSVAPRIVAVEQTNGQPLALKQGRPFVA
                     GRDSGQASAFVKVSQPGPLTILVTGLHRSTGSYQVDTTLVGDVNGDG"
     gene            complement(568..2058)
                     /locus_tag="VHS97_10020"
     CDS             complement(568..2058)
                     /locus_tag="VHS97_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3448578.1"
                     /translation="MTTDQIAVRDEPVAAKPRDNRRRTRSPKVRGWGWLGMILVVLAV
                     GGVGGLWLMASRNQQAKDGEKGSASDEGHEKKNLAKVQVIEPERGGMERVMSQPGTIR
                     AFEFAPLYTKVSGFIKTLNVDRGSRVKKGDLLAEIYDPERDVAVIQAEASLEHSKAEV
                     AQAKASILTAEASVKAAKAKQNEAKATLDQQLAARDYRNKAYQRISELVARGSAEQRL
                     KDEYLDEWHSAEAAVLSAQAGIETAAALLFEAQAKVEKAKADFKSAEAKVQVAAANLQ
                     MAKVFVQYTRIESPYDGVVIFRGEAVHPGSFVRAADQGGVNTPLLTVAMTEKMRTIVP
                     VPDRDVPYCQVGDPATVTLDAFPGKVFHGKVDRTAESETLDDRLMRVEIDLPNPDGLL
                     RDGMFCRADIILEKVVKNLTVPSSCLINRNGKGEGAVLVVRDGKIHRVNVHVGMDTGL
                     RAEIVDGLTDNDQVIVQPDPSIAEGTPVQVESAGSRSSATDPKKGE"
     gene            2377..2739
                     /locus_tag="VHS97_10025"
     CDS             2377..2739
                     /locus_tag="VHS97_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3448579.1"
                     /translation="MPMTDYQYITYKNVDGVAVVNFLESVAMFEGDKVNAVSDELIDL
                     VESKRFTKVLLNLSNAHFVSIAMLAHLVKLHRKAQEVKGKIRLCCLRPVIRDAFKVSN
                     FDKIFEIFADEAAALKKF"
     gene            complement(2798..4402)
                     /locus_tag="VHS97_10030"
     CDS             complement(2798..4402)
                     /locus_tag="VHS97_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3448580.1"
                     /translation="MSTSQDEESVMGVERPKTETSRPELIPHRWTRIDDPDSVAIVAA
                     HETVIAGGGPAGLTAAYELSKHGKSCVVLESDPRLVGGISRTDQYKGYRFDIGGHRFF
                     SKSEEVNQLWREILGEEFLKRSRLSRIYYDRKFFDYPLKPADALWKLGFVRSLRIGAT
                     YIQAKLAPIKPERSVEDWVVNRFGRVLFETFFKSYTEKVWGMPTSTISADWAAQRIKD
                     LDLVRAVTSALFGRFRSKEAAIIKTLIDEFHYPRLGPGQMWEVARDRIRGQGGVVQLD
                     RRVVEVEHDGGVVTSFLTRDHDGRRARYQGRHFLSTLPIRDLIRAMKPSAPAEVIHAA
                     ESLKYRDFLTVVLIVDKAETFPDNWIYIHEPQVRVGRIQNFKNWSPDLVPDSSKTSLG
                     LEYFCFEGDDLWTMPDADLQALGRREIDSIGLVSASTVVDGCVVRMPKAYPVYDDDYQ
                     AHVRVIRGWLKKLSNLELAGRNGMHKYNNQDHSMMTALLAARNILGMGHFDTWKVNTD
                     ALYHEGSTEESQVQKGRAVPKRVGAL"
     gene            4696..4962
                     /locus_tag="VHS97_10035"
     CDS             4696..4962
                     /locus_tag="VHS97_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448581.1"
                     /translation="MTYEQCQAILDDIRRRQGTERPLVQVTCGAAVMRGRVSYSASGL
                     AGRRNPNSPYGVLVLEEMGLARRPASFVQIANIPEDGLLSLDPA"
BASE COUNT          977 a         1527 c         1480 g         1047 t
ORIGIN      
        1 gccatcgcca ttgacgtcgc ccacgagggt ggtgtcgacc tggtacgatc cggtagagcg
       61 gtgtagcccg gtgacaagaa tcgtgagagg gcccggctga ctcaccttga cgaaagcgct
      121 ggcctgtccg gagtcgcgac ctgcgacaaa ggggcggccc tgcttgaggg caagtggctg
      181 cccgttggtt tgctccacgg ccacgatccg gggcgcgacc gaacttcctg gctccggttg
      241 gacaaaaatc gcaaaaagtg tcgaagactt gccctgagtc aggttcttgg gcgcgacggt
      301 gactgacgct gccgtgacct gtccaggggc ggtcaccgtc cccacgctcg tcccgatcga
      361 gtttgcgggt atcgccgcac tcaattgcag ccgaccctcg agccgttcga gcgagggccg
      421 gttgtcacgc cggcgactta aggacatcgg cgatcctccg tgacggcact tcgagccaga
      481 gcgcggaccg tgcagcatcc attatttgtt cggcaacggc ccgccttcga ctgaattcca
      541 attcgccggc gatcgttgcg ctgagaatta ttcgcctttt ttcgggtcgg tcgcgctcga
      601 cctagaacca gcggactcga cctggaccgg ggttccttcg gcgatcgaag gatcgggctg
      661 aacgatcacc tggtcgttgt cggtcaaacc atcgacgatt tccgcgcgaa gaccggtatc
      721 cattccgacg tgtacgttaa cacgatggat cttgccatcg cgtacaacca ggaccgcccc
      781 ttcgcctttg ccgttgcggt tgattagaca cgacgaagga accgtcagat tcttgacgac
      841 cttttcgagg atgatatcgg ctcggcaaaa catgccgtcc ctcagcaagc cgtcgggatt
      901 ggggaggtcg atttccaccc gcatcagccg gtcatcgaga gtctccgatt cggccgtgcg
      961 gtcgaccttg ccgtggaaga cctttcccgg gaaggcatca agcgtgacag tcgccgggtc
     1021 accgacctgg caatagggga cgtcgcgatc gggaaccggg acgatggtcc tcatcttttc
     1081 ggtcatggca acagtgagta gcggtgtatt gacacccccc tgatcggctg cgcggacgaa
     1141 cgagcctggg tgcacggcct caccgcggaa aataacaacc ccatcgtacg gtgactcgat
     1201 gcgcgtatat tgaacgaaga ctttggccat ctgcaggttg gctgccgcga cctgcacctt
     1261 ggcctcggcc gacttaaaat cggccttggc cttctcgacc ttggcctggg cctcgaataa
     1321 gagagccgcc gctgtctcga tccctgcctg cgccgacagg accgcggcct cggccgagtg
     1381 ccattcgtcg agatactcgt ccttgagccg ttgctcggcc gacccgcgcg cgaccaactc
     1441 cgaaatccgt tggtatgctt tgttgcgata gtcgcgcgcg gccaattgtt ggtccaaagt
     1501 cgccttcgcc tcgttttgct tcgccttcgc cgctttgacg gacgcctccg cggtcagaat
     1561 gctcgccttg gcttgtgcaa cttctgcttt cgaatgctcg agactggcct ccgcttgaat
     1621 gacggcgaca tcccgttccg gatcatagat ttcggcaagc aggtccccct tcttgactct
     1681 gctaccgcgg tcaacgttta gcgttttgat gaaaccggaa accttcgtat acaaaggtgc
     1741 aaactcgaat gcccgaatgg tccctggctg gctcatgact cgctccatcc cgccacgctc
     1801 gggctcaatg acctgcacct tggccaaatt ctttttctcg tgcccctcat cagacgcgct
     1861 ccctttctcg ccgtctttcg cctgctggtt cctcgaggcc atcagccaga gcccgccaac
     1921 tccgccaacc gcgagcacga cgaggatcat tccgagccaa ccccagcccc gcaccttggg
     1981 cgatcgtgtg cgtcgacgat tgtcacgagg ttttgcggcc accggctcgt ctcttacggc
     2041 aatctggtcg gtcgtcatca agccaccccc aagctcagaa aggcttgtca acctcgtgct
     2101 gcccgcaatg cgcactaacc tgccgatcgc gattcatcaa tttcgatgtt agggacttgg
     2161 gccaggatgg tcaatcatgg gcgatggcca cgaatgatcc gataaacacg aacacggctg
     2221 ggaaagacac tctggttttc ggtatgatct ggtgatccca tgcaattgaa ttggatgccg
     2281 ctggttgaca gcgccgaaat gggattctag tgaccggtag atgaaacgtt cgctgtcgtc
     2341 actcgctgtc gatgaactcg ccgaggagct tgacctatgc ccatgaccga ctatcagtac
     2401 atcacctaca aaaacgtcga tggcgtcgcc gttgtcaatt ttcttgaatc cgtcgcgatg
     2461 ttcgagggtg acaaggtcaa cgcggtttcc gacgaactga tcgacctggt cgaatcgaag
     2521 agattcacga aagtactctt gaatctctcc aacgcgcact tcgtttcgat cgcgatgctc
     2581 gcccacctgg tcaagctgca tcgcaaggct caggaggtca aaggcaagat ccgcctctgc
     2641 tgcctacgcc ccgtgatcag ggacgcgttc aaggtgagca acttcgataa gatctttgag
     2701 atcttcgccg atgaggccgc tgccttgaag aagttctgat cgtcggccgc tgtctaggtc
     2761 gtgatgatag tttcgaggtc tgtttgcgac ttcgacctta tagggcgccg acgcgcttcg
     2821 gcacggctcg ccctttctgg acctggcttt cctcggtcga tccctcgtga tagagggcgt
     2881 cggtgttgac cttccacgta tcaaagtgtc ccatgcccag aatgttgcgc gccgccaaga
     2941 gcgcggtcat cattgagtgg tcttgattat tgtacttgtg catgccgttg cgaccggcca
     3001 gctcgagatt ggagagtttt ttgagccagc ctcgaatcac ccgcacatgt gcctggtagt
     3061 catcgtcgta gaccggataa gctttgggca tgcggacgac acagccatca acgaccgtag
     3121 aggcggagac caggccgatc gagtcgatct cccggcgccc gagcgcctgc aagtcggcat
     3181 ccggcatcgt ccacaagtca tcaccctcga agcagaaata ctcgaggccc aggcttgtct
     3241 tgctcgaatc gggaacgagg tcgggcgacc agttcttgaa attctggatc cgaccgacgc
     3301 gcacctgggg ctcgtggatg tagatccagt tgtcaggaaa cgtctcggcc ttatcgacga
     3361 tgaggacgac ggtcaagaaa tcgcgatact tgagcgattc ggccgcatga atgacctcgg
     3421 ccggggcgga aggcttcatg gcgcggatca ggtcgcggat cggcagcgtc gacaggaaat
     3481 ggcgcccttg atagcgggcc cgccggccgt catggtcgcg ggtaaggaac gaggtaacca
     3541 ccccaccgtc gtgctcgacc tcgaccaccc ggcggtcgag ctggacaacg cctccctggc
     3601 cgcgaatccg gtcacgagca acttcccaca tctgacccgg gcccaggcgc ggatagtgga
     3661 actcatcgat caaagtcttg atgatcgcgg cctctttcga ccgaaaacgg ccgaaaaggg
     3721 ccgacgtgac agcccgcacc agatcgaggt ccttgattcg ttgcgccgcc cagtcggcgg
     3781 aaatggtcga ggtcggcatc ccccaaacct tctccgtgta cgacttgaag aaggtctcga
     3841 agagcacccg gccgaaccga ttgacgaccc aatcctcgac gctgcgctcg ggcttgatcg
     3901 gtgcgagctt tgcctgaatg taggtggcac cgatccgtag cgatcgtacg aagccgagct
     3961 tccagagcgc gtcggcgggt ttgagcgggt aatcgaagaa tttgcgatca taatagatgc
     4021 gggagagccg cgagcgcttg agaaactctt ccccgaggat ctcgcgccag agttggttga
     4081 cttcttcgct ttttgagaag aatcggtggc cgccgatatc gaagcgataa cctttgtact
     4141 ggtcggtccg actgattccg ccgaccaagc gtgggtcgga ttcgagaacg acgcaggatt
     4201 tgccgtgctt cgagagctcg taggcggcgg tgaggccggc gggcccgcca ccggcaatga
     4261 cggtctcgtg cgccgccacg atcgcgacgg agtccggatc gtcgatccgc gtccagcgat
     4321 ggggaatgag ctccgggcgt gacgtctctg tctttggccg ctccacgccc atgaccgact
     4381 cctcgtcctg agaggtcgac aacccctgct cttttttcgc cgcggcggga tgccgactgc
     4441 taccattcct gaaatcggct accgagcgtc gcgagttttg gcataaatcc gctcgggggt
     4501 caacgccgat catattccgt agggcccctg tcgcacttaa cgtccgggtc tcgaatctca
     4561 aaatgacgcg agtcaacaga agcggctcga gctgcaggcc gcccgacagg gcggacccac
     4621 atggaagtgg gagcggaaaa cgcccgatgg ctcacgagta gattgtcgtt tgctgggaac
     4681 cagggagggt ccaggatgac ttatgaacag tgccaggcga ttcttgacga tatccgtcgg
     4741 cgtcagggga ccgaacgtcc cctcgttcaa gtaacttgcg gcgccgcggt aatgcgggga
     4801 cgggtcagtt attccgcgag cggccttgcc ggccgccgca atccgaactc gccatacgga
     4861 gttctcgttc tcgaggagat gggcctggcg cgccgacctg cgtcgttcgt gcaaatcgcc
     4921 aacatacccg aggacggtct cttgagcctc gatccggcat gagatgaggc acgcaggcga
     4981 ttgcctcccc acacacgaac cctcccccac aaggggggag ggcgccgaac c
//
LOCUS       DATAQL010000578         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2196698, whole genome shotgun sequence.
ACCESSION   DATAQL010000578 DATAQL010000000
VERSION     DATAQL010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196698"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VHS97_10040"
     CDS             complement(<1..392)
                     /locus_tag="VHS97_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3448582.1"
                     /translation="MSVTELPVIDRSWLPALEPRELYEALTRAISTHDVRYDRLSRAL
                     YATDASVYQIVPLMVAFPATADDVSAAVRVCAQFGVPITARGGGTSQAGQSIGPGVIL
                     DCSRHFDRVLEINPLERWARVEPGCVLD"
     gene            complement(416..1591)
                     /locus_tag="VHS97_10045"
     CDS             complement(416..1591)
                     /locus_tag="VHS97_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3448583.1"
                     /translation="MRPTAVNRVLQEVRQLQAGGQSLISLMRGQPDTPTPVHIVEAAT
                     KSLRDGRTGYPDNQGEPALRRAVALKLKRDQGLDYDPDREILITDGATLGVCAALGAL
                     VDQKKSVLLPDPIYDAYEGPIALWGCRPAPVHATLRDGRFTFGSRQLLDSRVAGKNVI
                     LLNTPWNPVGTVLSHEELVDIMGFAERNDYMVISDEIYESLIYDGRRHISPASVSTDA
                     RERTILLNSLSKTYAMTGWRVGYCAGPEEIIRAMLLVLQQSSRGPATFVQDAAACALT
                     SDQECARLMALEYQGRRDRVVEFLRGIPGVEPRVSEGGLFVMVDIRGLGRPADDVRKF
                     LLSEAGVVVIHGSAYGPGGEGFLRVSFAAGGETLHRGLERLRDGLITLADGAITRIA"
     gene            complement(1677..2954)
                     /locus_tag="VHS97_10050"
     CDS             complement(1677..2954)
                     /locus_tag="VHS97_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX3448584.1"
                     /translation="MTIDLAWISLAALVLVIVLSCTTVVNPGFVSLALAWVIGIYLAP
                     LWGRRFTIGEVLAGFPTDLFLTLVGVTLLFTLARGNGTLDQVVRAALGLCRGNTRLIP
                     IMFFGLSLAIASIGAGNIASAALIAPLAMAVADRAKISPLLMTLMVGHGCIAGALSPI
                     APTGIIANGLMSRMGMSGFEQQNYLYNLMANALAAGTAYLGFGGLRLWRQTADDERSG
                     HGTSGPAATMEWKHWLTLGAIAMLVTGVVGFQVHVGMGAIAAAALLTLAKVTDEKPAI
                     LTIPWGVIVMVCGVTVLTSLLEKTGGIDLFTTILAQFATHRSVTGLIALVTGLISVYS
                     STSGVVLPALLPSVPGLVAKLGGGNPLAIASSILIGGHLVDVSPLSTVGALCIASTPA
                     SADRRLLFNQVLAWGLSMSVVGAAGCYLVFGRW"
     gene            complement(2969..>3403)
                     /locus_tag="VHS97_10055"
     CDS             complement(2969..>3403)
                     /locus_tag="VHS97_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX3448585.1"
                     /translation="NLFRDYWRKPEASREAFASGWFDTGDLGSRDASGFLTLVGRKND
                     LIITNGFNVYPQVVERVINDCPGVRESVVVGIADKKRGERVVAAVVRSDETLTLQAIR
                     TFLGERLVDYQRPAEVILVAALPRNSMGKVLRRELRDQLAGN"
BASE COUNT          732 a         1096 c          995 g          580 t
ORIGIN      
        1 tcgtcgagca cacatcccgg ctcgacccga gcccagcgct cgagaggatt gatctcgagc
       61 acgcggtcaa agtgtctcga acagtcgaga atcacccccg gaccgatcga ctggccggcc
      121 tgagacgtac caccgccccg agctgtgatc ggtacaccga actgtgcgca cacgcgcacg
      181 gcggctgata cgtcgtctgc cgtcgcaggg aatgcgacca taagtggaac gatctggtag
      241 acgctggcat ccgtggcata gagtgcccgg ctgaggcgat cataccggac gtcatgcgtg
      301 ctgatagctc gagtgagtgc ttcgtaaagc tcacgaggtt ctagcgctgg cagccaagag
      361 cggtcgatca cgggcaattc cgtcacactc atgcgaggtc cttgacgaat acgtctcagg
      421 cgatccgggt tatagctccg tctgcaagcg tgatcagacc gtcgcgcaag cgctccaaac
      481 cccggtgcag cgtttcgccg cccgcagcaa aggacactcg caagaagcct tctccacccg
      541 ggccataagc cgagccatgg atgacgacga ctcccgcttc actgagcagg aacttccgga
      601 cgtcgtctgc cggcctccca aggccacgga tgtcgaccat cacgaacaat cccccctcgg
      661 aaacacgcgg ctcgactccg ggtatgccgc gcaggaactc gacaacccga tcgcgcctac
      721 cctggtattc caatgccatc agtcgtgcgc actcttgatc cgaggtcagt gcacaggccg
      781 ccgcatcttg cacgaatgtg gccgggcccc ggctcgactg ctgcaagaca agaagcatcg
      841 cgcggatgat ctcttcggga ccggcgcagt aaccgacgcg ccagccggtc atcgcataag
      901 tttttgagag gctgttgagt aggatcgtcc gctcgcgcgc atcggtggaa acagacgccg
      961 gcgagatgtg ccggcggcca tcgtaaatca gcgactcgta gatctcgtca ctgatgacca
     1021 tgtagtcatt tctctcggcg aaccccatga tgtcaacgag ctcttcgtga gagagtacgg
     1081 ttcccacggg gttccagggc gtgttgagca atatgacgtt tttgcccgca acccgtgaat
     1141 caagaagctg ccgactaccg aacgtgaatc ggccgtcgcg cagtgttgca tgaacaggcg
     1201 ccggcctgca cccccagagt gcgatcggcc cttcataggc gtcataaatc ggatcgggga
     1261 gcaagacgct ctttttctgg tcaaccaatg cgcccagggc cgcacatacg ccgagcgtgg
     1321 cgccgtcggt gatgaggatc tcgcggtcgg gatcgtagtc gagcccctgg tcccgcttca
     1381 gcttgagggc gacagcgcgg cgcagggccg gctcaccctg gttgtcgggg tagccggtgc
     1441 ggccatcgcg gagggacttc gtcgccgctt cgacaatatg cactggcgtc ggcgtgtcgg
     1501 gttgcccgcg catgagcgag atcagcgatt gcccgccggc ctggagttgc cggacctcct
     1561 gcaagacccg attaacggcc gtcgggcgca agcgagagac tcggtcagcg aaaggcacat
     1621 aagccatggc cattgctcac gagaaaggaa cgacaggttc tacgccttct gccactttac
     1681 catcttccaa agactaggta gcagcccgcc gccccgacaa ccgacatcga cagcccccat
     1741 gccaggacct ggttgaacag gagccggcga tcggccgaag ccggcgtgga agcaatgcac
     1801 agcgccccga ctgtagacag cggcgaaacg tcgaccaagt ggccgccgat caagatcgac
     1861 gacgcaattg ccagcgggtt gccacctccc agcttggcaa caaggcccgg gacgctcggc
     1921 aggagagccg gcaacaccac gccggacgtg ctgctgtaaa ccgaaatcag acctgtcacc
     1981 agcgcgatca ggcccgtgac cgagcggtgc gtcgcaaact gcgccagaat cgtcgtaaac
     2041 agatcgattc ctcccgtctt ttcgagcaga gacgtcagga ccgtcacgcc gcagaccatc
     2101 acgatcacac cccacggtat tgtcaggatc gcgggctttt catcggtgac cttggccagc
     2161 gtgagtaacg ccgccgccgc gatggcaccc atcccgacgt ggacctgaaa accgacgacc
     2221 ccagtcacga gcatggcgat ggcccctagg gtgagccagt gtttccattc catcgtcgcc
     2281 gccggaccag acgtaccgtg accggaccgc tcgtcgtcag cggtctgtct ccaaagccgc
     2341 aggccgccaa agccgaggta agccgtgccc gccgccaagg cattggccat gagattatac
     2401 agatagtttt gctgctcgaa tcccgacatg cccatgcgag acatcaatcc attggcgatg
     2461 attcccgtcg gggcgatcgg tgaaagagct ccggcaatgc agccgtgccc caccatgagc
     2521 gtcatgagca atggagagat cttggcccga tccgcgacgg ccatcgccag gggagcgatc
     2581 aaggcagcgc tcgcaatgtt gcccgctcca atcgaggcaa tcgcaaggga aagcccgaag
     2641 aacataatag gtatcagcct ggtgttccct cgacatagtc cgagcgctgc gcgcacaacc
     2701 tggtccaggg tgccgttgcc acgtgctagt gtgaacagga gcgtcactcc caccagcgtc
     2761 aggaacaggt cggtgggaaa accggccagt acctcgccaa tcgtgaaccg ccgtccccac
     2821 aagggcgcaa ggtaaattcc gatgacccat gccaaggcca gcgagacgaa tcccggattg
     2881 accactgtcg tgcaggacag cacgatcacc agcacgagtg ccgccagcga gatccatgcg
     2941 aggtcgattg tcacgtcgtt gatagttctc aattgccggc gagctgatct cgcaattcgc
     3001 gccgcaagac ctttcccatg gaattacgtg gcagggcagc gacaagaatc acctcggctg
     3061 gccgctggta atcaaccagc cgctcaccga gaaaagtccg gatcgcctgg agggtcagcg
     3121 tctcgtcgct ccgaacgaca gccgccacca cgcgttcgcc gcgcttcttg tcggcgatgc
     3181 ccaccacgac ggattcccgc acgccggggc aatcgttgat cacccgctcg acaacctgag
     3241 gatatacgtt gaatccgttg gtaatgatca gatcattctt acgccccacc agggtcaaga
     3301 atccggatgc gtcgcgagag cccaagtcgc cggtatcgaa ccagccggaa gcgaaagcct
     3361 cgcgactcgc ctccggcttt cgccagtaat cccggaacaa gtt
//
LOCUS       DATAQL010000579         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1891339, whole genome shotgun sequence.
ACCESSION   DATAQL010000579 DATAQL010000000
VERSION     DATAQL010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891339"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(993..1430)
                     /locus_tag="VHS97_10060"
     CDS             complement(993..1430)
                     /locus_tag="VHS97_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448586.1"
                     /translation="MAKKPEPPKPIGWSIYKIASKAVLAWRDRCDGRSRCDREGRNRI
                     QGAGQQADGATAMTRRKGEITRSDLEREWPHHVALPAEMGLGLKNSELVRSVAASLSA
                     APLTYSLRGDDLRLVVFCFTKPEDARAFCEQFGGERLPTGSRR"
     gene            1429..>1670
                     /locus_tag="VHS97_10065"
     CDS             1429..>1670
                     /locus_tag="VHS97_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448587.1"
                     /translation="MPAVSVASYGGQLGGSATGGSAGLTVQRSVGQSAAHDAVVHAAA
                     AVVGSGTDGPETDGSAGTNSEVNSEGKRATGMAAGTT"
BASE COUNT          319 a          519 c          486 g          346 t
ORIGIN      
        1 acaactaccg agcctcgccc gttggtaagt acaccaatcg gatgttgtct cgacttggcc
       61 cccttctaag cggggccatt tttcttggcg tgctgcgacc ctctcgccat tgccctgacg
      121 gccacggctt cggcacggcg attaaccgct gcctgaaacc acatttggtc atttggtccg
      181 cactaccttc gaaagctgtc gtggccaggc gatcgatggt cacagcagct aagggccaat
      241 ggactaaacc gctctggcgg tagggtgctg cgaggcccac tgagcgcggc cctaaataaa
      301 ttcccgaatg ggagcatctg ttcgagcggt ctcgacaacg tcactcaaga caggagatca
      361 tccccatcac cgagcgttgc gtcggcggat gatcaaaggc ctggcgatcc acacgattga
      421 tgccgaacac tctgcaaggc taccaacgac attgaacgtc acacggtgca gcggcagatg
      481 cgaagatcat cgagagtaag gcagctcgag actgcaggct tcaattcgat tgctcggcct
      541 gtccatcgtt gcgaacctca caactcggaa gttgtagcat gtgatatttt tgcaacgcct
      601 aactctttga aatcgcgtgt ggcccgaaac ctcaaatccc catttggcaa tatccgtgcc
      661 gtaccgttta agttgtgccc tcatccgagg ccaacgaccg ttcaaaattg cctctcattg
      721 ggatcgcgcg tggctctcaa atccccacag ctgagcgcgc gggcaccgcc caatatgtac
      781 cctttcccgc ggtttcgtcc cttggaggtt tccagacgcc ggccgccgtc ggtgtgggaa
      841 tgtttgtcac cgccggcgtc tggaaacctt cacactttga gaccacatcg gaggctacat
      901 cgttgccgat ctattggtcc ggtcctccta catgagttga cgtttgcgtt tgtcctgaac
      961 aacgcgctcg ggtcacccgc ttgctttcat ggtcaccgcc ggctgcccgt cggcaaccgc
     1021 tcgccaccga attgctcgca gaacgctcgc gcatcctccg gcttggtaaa gcagaacacc
     1081 accagccgga gatcatcccc gcgcagggag taggtcaacg gggccgccga taaacttgcc
     1141 gcaacgctgc gtacgagttc gctgttcttg agacccaacc ccatctcagc cggtagggcg
     1201 acatggtgcg gccactcgcg ctcaaggtcg gagcgggtga tttcgccctt gcggcgggtc
     1261 atcgccgtag cgccatcagc ctgttggccg gcaccttgaa ttcggttgcg cccttctcga
     1321 tcgcagcggc ttcgtccgtc gcatcgatct cgccaagcca ggacagcctt gctggcgatc
     1381 ttgtagatgc tccacccgat gggcttcggc ggctctggtt tcttggccat gcccgcagta
     1441 tcggtcgcta gctatggagg tcaattgggc gggtccgcga cgggcggctc agcaggactg
     1501 acagtgcagc gctcggtcgg gcagtccgca gcgcatgacg cggttgtgca cgccgccgcg
     1561 gcggtggttg gctctggtac ggacggccca gagacggacg gctccgcagg aacgaactcg
     1621 gaggtgaact cggaggggaa aagagcgacc ggcatggcgg cgggaacgac
//
LOCUS       DATAQL010000580         5706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4181501, whole genome shotgun sequence.
ACCESSION   DATAQL010000580 DATAQL010000000
VERSION     DATAQL010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4181501"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VHS97_10070"
     CDS             complement(<1..281)
                     /locus_tag="VHS97_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448588.1"
                     /translation="MIDRGTSLAFFRGSELPRLLVLAVVMVAGWGLFLNFANRQPDAV
                     EPALTAGDNPEPIVADRSVEFESVTDRTPVEFRDNAAYAMLLKRARGHSP"
     gene            complement(278..784)
                     /locus_tag="VHS97_10075"
     CDS             complement(278..784)
                     /locus_tag="VHS97_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3448589.1"
                     /translation="MSEQKKVRRAQAPDVPIAPMLDMAFQLLTFFVLTYRAAPSEVQF
                     GMQLLPPQPATAITAEAPSDKPSDNLPVSLRTLPTILRAGERGSLAKVTIGETSIPTD
                     PKLIEQELDKIFQNPDLPFDQTIIRFDPQLKYSELMRVINAFSNAFVHAKKEPKISFD
                     ELVPNEGE"
     gene            complement(821..1288)
                     /locus_tag="VHS97_10080"
     CDS             complement(821..1288)
                     /locus_tag="VHS97_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3448590.1"
                     /translation="MAAIMSSEFKAEPNLTPLLDVVFQLITFFMLVINFSSENYDQRV
                     RLPVAESARPVDDEARVSEDRLVLNVDKEGHLLINGEVQPLHKAIQTIRHQADLVKLN
                     LRAGGVKTQPTGTLPTTIILRVDRDATCASLLSLIKACESQGFRKFRFKAMNS"
     gene            complement(1322..2224)
                     /locus_tag="VHS97_10085"
     CDS             complement(1322..2224)
                     /locus_tag="VHS97_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3448591.1"
                     /translation="MHQLLPFRRSDKTERRWLLFCLLSGLLVWAAWEGHGLVNAQEAA
                     KPDAAAKDAPPAKEEKADAAPAEPEGAAKSAAAPTGNGTAPPARENMLQWAIRASGPI
                     GAFLLVLSIYFTALVIRLFMEFRITEAVPPALVEKLETAIRDKKFQDAYDVCKDNDSF
                     LARLVRTGIANLPNGRAEAKEAMETATEEIVTSLEMKISYLAIIGTLGPMIGLVGTIW
                     GMIMSFQEIATAAGAQPRPEKVAEGISTALFITLEGVSLSVPAIFFFAYFRNRIAQMT
                     MEASRVADRTITSLIAAAKTVKAS"
     gene            2511..3968
                     /locus_tag="VHS97_10090"
     CDS             2511..3968
                     /locus_tag="VHS97_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3448592.1"
                     /translation="MTALVLSSCLVLAWADPPPQQPNQPALSPTDVVAALETAIADAI
                     AKAEPSVVAIHRFKGENTQTTQAVRGRKRPLSLHEPPRRPGIDLRFARPAERPNLISF
                     DFGSGVVIGPEGQILTAYHVVKGARQLTVRAADRQEFEAEIIAADPRSDLAVIAPSEN
                     DGGDPPKLKPLAIGDAGSLRKGAFLIALGNSFNAARDGKPSASWGILSNVARKLDSEL
                     DEMSMARRQPRLPNFPTLLQLDSKLNLGMSGGAVINLKGELVGLTTMAASPAGFDAMA
                     GYAIPMDKMGKRNVETLKEGKEIEYGLLGIIADNGYTNHVGKVQPNSPAALGDLQSDD
                     EIIAVDDVRVYDFDTLVLAVNAYSAGDTIRLKIRRGEETISRSIVLAKVAVDGEIIAT
                     NRPKPWRGLRVDYTSALNSPTFGANFLESAITGVVVAEVEEGSPAAAAGMKKGQLIRK
                     VGDHSVRSPRDFAQAIATLDGPVTLNTDLGLVTIP"
     gene            4078..>5706
                     /gene="alaS"
                     /locus_tag="VHS97_10095"
     CDS             4078..>5706
                     /gene="alaS"
                     /locus_tag="VHS97_10095"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX3448593.1"
                     /translation="MKTDELREAFLDFFVTKGCVRRPSDVLVPNDPTVLFTPAGMNQF
                     KKEFMGLGDPHFKRATTCQKCIRTGDIENVGKTAFHETFFEMLGNFSFGDYFKREAIH
                     WAWEFLTGTLKIPADRLTVTVYLDDDVAYDIWHNEIKIPAQRISRMGEDDNFWPAGAP
                     THGPNGVCGPCSEVFYHGAGPKEVEIWNLVFTQFNRTGPGQLEPLPNKNIDTGMGLER
                     AAACLQGVSTVFDIDIFKPIVAAVADSLAVKYDSDHPDGVRIRRMADHIRALTFCIHE
                     NVRPGPEKQGYVIRRLLRRAVLDAYQMGRRLPFLHQLVPVVAEVMNKPYPDLHESVSR
                     VQTTIKQEEEQFLKNLENGLGLLTDIFQKTKSAGSEIISGKDAFTLHATYGIPVEVVE
                     SLATDHNLRIDKAGFEAERGRHTVISLGTTEAHYVFFTGPLDTLKREYHHGSEFVGYS
                     TTHEQARIIGILEQNRLAERAQGNGGTSLVVILDRTPFYGESGGQVGDIGLISGDRFS
                     FQVVDTKKENDFVLHIGHVAAGELTVNDPVRAQVD"
BASE COUNT         1171 a         1744 c         1643 g         1148 t
ORIGIN      
        1 ggcgaatgcc ccctcgctcg tttgagcagc atcgcatacg ccgcattatc gcgaaactcg
       61 accggtgttc ggtcggtaac tgactcaaac tccacggaac gatccgcgac gatcggctcg
      121 gggttgtccc ccgcggtgag agccggctca acggcgtccg gctgccgatt cgcaaaattc
      181 aggaatagcc cccagcccgc gaccatgacc accgccaaca ccaatagccg ggggagctcg
      241 gaaccacgaa agaacgccaa gctcgttcca cggtcaatca ttctccttcg ttgggaacca
      301 gttcgtcgaa ggagatcttg ggttctttct tggcgtggac aaacgcattt gaaaatgcgt
      361 tgatcaccct catcagctcg gaatatttga gttgtgggtc gaacctgatg attgtctggt
      421 cgaagggcaa gtcggggttc tgaaaaatct tgtcgagctc ttgctcgatc aacttggggt
      481 ccgtcggaat cgacgtctct ccaatcgtga ccttggcgag cgagccgcgt tcgcccgcgc
      541 gaaggatcgt cggcaaggtc ctgagcgaga ccggcagatt gtctgacggt ttatccgatg
      601 gcgcctcagc cgtgatcgcg gtggcgggtt gcgggggcag caactgcatg ccgaattgca
      661 cttccgacgg agcggctcta taggtcagga cgaagaacgt caagagctga aacgccatgt
      721 cgagcatcgg cgcgattggc acgtcggggg cctgggctct ccttaccttc ttttgttccg
      781 acaaggcctt agctcccgcg gtgtccgagc ccaaggcgac tcatgaattc atcgccttga
      841 agcgaaattt tcgaaatcct tgagactcac atgctttgat caggctcagc agcgatgcac
      901 acgtcgcgtc gcgatcgact cggagaatga tggtcgttgg cagggtcccg gtgggctgtg
      961 tcttcactcc gccggctctg aggttgagct tgaccaggtc ggcttgatgc ctgatcgtct
     1021 ggatcgcctt gtgcagcggc tgaacttcgc cgttgatcaa gaggtggccc tctttatcca
     1081 cgttcaagac taatcgatct tccgaaacac gggcctcatc gtcgacgggt cgagccgact
     1141 cggcgaccgg caggcggacg cgctgatcgt agttttcgct actgaagttg atcaccaaca
     1201 tgaaaaaagt gattaactga aacacgacgt cgagaagagg tgtcagattg ggttcggcct
     1261 tgaactcgct gctcatgatc gcggccacga ctagatctcc cttgggaccg accggctcgt
     1321 ctcaagacgc cttgacggtc ttggccgcgg cgatcaagga ggtgatcgtg cgatcggcca
     1381 cgcgcgatgc ttccatggtc atctgggcga tgcggttgcg aaagtacgcg aaaaagaaaa
     1441 tggccgggac agagagggat acgccttcaa gggtgataaa tagagcagtg gagatacctt
     1501 cggcaacctt ttcgggcctg ggctgggcac cggcggccgt ggcgatctcc tgaaagctca
     1561 tgatcatccc ccaaatcgtg ccgactaatc cgatcatcgg tcccagcgta ccgatgatcg
     1621 ccagatagct gattttcatt tccaggctgg tcacgatttc ttcggttgcg gtctccatcg
     1681 cctccttggc ttcggctcga ccgttgggca agttggcgat gcccgttcgc accagcctgg
     1741 ccagaaagct gtcgttatcc ttgcacacat cgtaggcgtc ttggaatttt ttgtcgcgaa
     1801 tcgcagtctc gagcttttcc acaagcgccg ggggcacagc ttcggtgatc cgaaattcca
     1861 tgaacagccg gatcacgagc gccgtgaagt aaatcgataa cacgagcaga aacgcaccga
     1921 tcggcccgga cgcccggatc gcccattgca gcatgttttc gcgggcgggc ggcgcggtcc
     1981 cgttgcctgt cggagcggcc gcgctcttgg ccgcaccctc gggctccgcg ggtgccgcgt
     2041 cagctttttc ttccttggcg gggggcgcgt ctttcgccgc cgcgtcaggt tttgccgcct
     2101 cttgtgcgtt gaccaggccg tgtccttccc acgccgccca aaccaagagc ccactaagca
     2161 agcaaaaaag aagccaccgt cgctcggttt tgtcgctgcg tcggaaaggc agtagctgat
     2221 gcatcgaaga tttctccttg tctatcatca ctatccgacg gcggtctcac actgtcaact
     2281 cctcacgcgg gctggcacca cgctttagtg aaatcaagtg gatcccgcag aaaagcccct
     2341 attatttaca atcgcaaacc gcatcgcaaa ccgcgcgttt gcgcgctccg gcgggatctt
     2401 gggttgctca ccaagcccca accggaaatg atggatgagg tgtgcgccgg ccgcaacttc
     2461 tcgatggcac acacatccgg cgcccttgaa cgagaacgaa ggacccgatc atgaccgctc
     2521 tcgtcttgtc ttcatgcttg gtcttggcat gggctgaccc accgccccaa caacccaacc
     2581 agcccgccct ttccccgacc gacgtcgtag cggcgctcga gaccgccatc gccgacgcca
     2641 ttgccaaggc cgagccgtcg gtggtcgcga ttcatcgttt caagggggaa aacacccaga
     2701 cgacccaggc ggtacgtggt cgcaagcgtc cgttgagttt gcacgagccg ccgcgacggc
     2761 ccgggatcga tttgcgtttc gctcgccccg ccgagcggcc caacctgatc tcgtttgatt
     2821 tcgggtcggg cgttgtcatc gggccggagg gccaaatcct cactgcctat catgttgtca
     2881 aaggagcgcg ccagctgacc gttcgggcgg ccgaccggca ggaatttgaa gccgagatca
     2941 tcgccgccga tccacgaagc gacctggctg tcatcgcccc cagtgagaac gacggaggcg
     3001 accctcccaa gctcaagccg ctggcaatcg gcgacgcggg ctccttacgc aaaggggcgt
     3061 tcctgattgc gctcggtaac tcgttcaacg ccgcccgcga cggcaaacct tcggcaagct
     3121 gggggatctt gtccaacgtt gcgcgcaaac tcgactcgga actcgacgag atgagcatgg
     3181 cgcgtaggca gccgcgtctg ccgaacttcc cgaccctctt gcaactcgat tccaagctca
     3241 acctgggaat gagcggcggc gcggtgatca acctcaaggg ggaactggtc ggcttgacca
     3301 cgatggcggc aagtcctgcg ggattcgatg ccatggccgg gtacgcgatc cccatggata
     3361 aaatgggcaa gcgtaacgtc gagacgctca aggaaggaaa ggaaatcgag tacggcctcc
     3421 tgggcatcat agcggataac ggctatacca atcacgtcgg taaggttcag cccaactcgc
     3481 cggcggcgct cggcgacctt cagagcgacg acgagatcat cgccgtcgac gacgtgcgcg
     3541 tgtacgactt cgatacgttg gtgctggcgg tcaatgccta ttccgccggc gacaccatcc
     3601 ggctcaagat ccgtcgcggc gaggaaacca tcagtcggtc gatcgtcctt gcgaaggtcg
     3661 cggtcgatgg tgaaatcatc gccactaacc gacccaaacc gtggcgcgga ctgcgcgtcg
     3721 actacacgag tgcgcttaac tcgcccacct tcggcgccaa ctttctcgag tccgcaatta
     3781 cgggcgtcgt ggtggcggag gtggaagagg gatcaccggc cgccgccgcc gggatgaaga
     3841 agggccagtt gatccgaaag gtcggcgacc acagcgtccg gagcccacgc gacttcgctc
     3901 aggcgatcgc cacgctcgat ggtccggtca ctctcaacac ggacctgggc ctcgtcacga
     3961 tcccatgaag ccgttctcgg cttgcctccg ccccgcttgt ggcccaaaat gtcgctcgga
     4021 attaatgttg caactccgga ggagtagggg gcttgcgccc gtttttgctg attcaccatg
     4081 aaaacagacg aacttcgcga ggcgttcctc gactttttcg tgaccaaggg gtgtgttcgc
     4141 cggcccagcg acgtgctcgt gcccaacgac cccaccgtcc tttttacccc ggccggcatg
     4201 aaccagttca agaaagagtt catggggctg ggcgatcctc acttcaagcg cgcgacaact
     4261 tgccaaaaat gcattcgcac cggcgacatc gaaaacgtgg gcaagacagc ctttcacgag
     4321 acgtttttcg agatgctggg caatttcagc tttggtgact acttcaagcg tgaggcgatc
     4381 cactgggcct gggaattctt gaccggtacc ctgaagatcc ccgccgaccg gctgacggtc
     4441 accgtctatc tcgacgacga cgtcgcatac gacatctggc acaatgaaat caagatccca
     4501 gcccagcgga tttcacgcat gggcgaggac gacaatttct ggccggccgg cgcgcccacg
     4561 cacgggccca acggcgtgtg cgggccatgc tcggaggtct tttaccatgg tgctggaccc
     4621 aaggaagtcg agatctggaa cctcgtcttt acccaattca accggaccgg ccccggccag
     4681 cttgaacctc ttcccaacaa aaacatcgat accggcatgg ggctcgaacg agccgcggca
     4741 tgtctgcagg gtgtttcaac cgtctttgac atcgatatct tcaagccgat cgtggcggcg
     4801 gttgccgact ctttagccgt caaatacgat agcgatcatc ccgacggagt ccgtatccgc
     4861 cgcatggcgg accacatccg ggccttgacc ttttgcatcc atgaaaacgt ccgccccggg
     4921 cccgaaaaac aaggatatgt gatccgccgc ttgcttcgtc gcgccgtgct cgacgcctac
     4981 cagatgggac gccgcctacc gttcctccac cagctcgttc cggtcgtggc ggaagttatg
     5041 aacaagcctt atccggatct gcacgagagc gtgagccgcg tccagaccac aatcaagcaa
     5101 gaggaagaac aattcctcaa gaatctggaa aacggccttg ggctcttgac cgacattttc
     5161 cagaaaacaa agtcggcggg ctccgaaatt atttccggca aggacgcctt caccctccat
     5221 gcgacgtacg gcattcccgt cgaagttgtc gagagcctgg cgaccgacca caacctgcgc
     5281 atcgataagg ccggttttga ggctgagcgc ggccggcaca cggtcatttc tcttggcacg
     5341 accgaggccc actatgtctt tttcaccggc ccacttgata cgctcaaacg agagtaccac
     5401 cacggcagcg agttcgtcgg ctactcgacc acccacgagc aggcacggat tatcgggatc
     5461 ctcgaacaaa atcgcctcgc cgaaagggct cagggcaatg gtggaacttc gctggtcgtc
     5521 atcctcgata gaactccctt ttacggcgag tcgggtggcc aagttggcga tatcggcctg
     5581 atcagcggcg accgcttcag ttttcaggtt gttgacacga aaaaagaaaa tgattttgtc
     5641 ctgcacatcg gccatgtcgc cgcaggcgag ctgacggtca atgacccggt ccgcgctcag
     5701 gtggat
//
LOCUS       DATAQL010000581         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5097173, whole genome shotgun sequence.
ACCESSION   DATAQL010000581 DATAQL010000000
VERSION     DATAQL010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5097173"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VHS97_10100"
     CDS             <1..441
                     /locus_tag="VHS97_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448594.1"
                     /translation="TGLGTAMRATGDNPRMIRSLGVDVGRMITLGLRLSNALVALCGA
                     ILAQYHGFADIQMGIGMIVWGLASVILGQALVGTKGLGLSITGAVLGSLLFRLMVALA
                     LHWGLDPIDLKLITAAFVFAALVLPKVFAGRTRPTAGALPSGSV"
     gene            444..1262
                     /locus_tag="VHS97_10105"
     CDS             444..1262
                     /locus_tag="VHS97_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3448595.1"
                     /translation="MALLELRGVYKTFNRGTPDEVRALRGVDLTVEQGSFVVVIGTNG
                     SGKSTTLNSVAGSFPVDAGTIRLAGREITRWPEHRRAKLIGRVFQDPLAGTSPSLSIG
                     ENLALASRRGLPRGLGRAVTRRKVAEFRDRVRPLGMGLEDRLDNPIGSLSGGQRQALT
                     LLMATYRRPELLLLDEHTAALDPKSAEQVIRLTDQIVRRQHQTTLMVTHSMAHAASLG
                     DRLIMAHQGRLTFDVSGAAKLRLRPHDLLEKFEELRRSDQLDETAARLLRDVYV"
     gene            1280..1528
                     /locus_tag="VHS97_10110"
     CDS             1280..1528
                     /locus_tag="VHS97_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3448596.1"
                     /translation="MKQRIEPGGYYAIAARAARLPVAEVYRWTLREPLPHLPIPLREP
                     DPDLLIDLATLVSRVQQRSRYERTLRSATPGPSAKRHR"
     gene            1806..2147
                     /locus_tag="VHS97_10115"
     CDS             1806..2147
                     /locus_tag="VHS97_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448597.1"
                     /translation="MTEKEPCQAELKFIGSMTGDNLQVLEVCLIYGTRAGEESENSLV
                     IRSIGDFGQSIVAAWNLVREGLINPVKRELRFVIESTVKYLYVDQQIRNGATIPKLPD
                     RLDSLFARSAA"
     gene            2274..>2651
                     /locus_tag="VHS97_10120"
     CDS             2274..>2651
                     /locus_tag="VHS97_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448598.1"
                     /translation="MSIVRGGNAVLAAGPKRDVTRVKPINASTTVLRNTVDTNKAYHA
                     ALQPNSIWQFYQLVMTQWPLTPNSPTTPGDPAHTFPGSPPNDQSAFSNVTLETFDQGS
                     IFTGCMACHNATMQPTDFLWSLND"
BASE COUNT          490 a          878 c          777 g          506 t
ORIGIN      
        1 acgggcctcg ggaccgctat gcgggctacg ggagacaacc cccggatgat ccggtcattg
       61 ggcgtcgacg tgggccggat gattactctc gggctgaggt tgtccaacgc gctggtcgcg
      121 ctttgcggcg cgatcctggc gcagtaccat ggtttcgccg acatccagat gggcatcggc
      181 atgatcgtct ggggcctggc gagcgtcatc ctgggacaag ccttggtcgg cacgaagggc
      241 ctgggcctat cgatcacggg cgcggttttg gggtcgctcc tgttccggct gatggtggcg
      301 ctcgcgcttc actgggggct cgacccgatc gacctgaaac tgatcacggc cgccttcgtg
      361 ttcgcggccc tcgtgctgcc caaggtgttc gccggccgca ccagacccac cgccggggcc
      421 ttgccgtctg ggagcgtctg atcatggcac tgctcgagct gcgcggggta tataagacgt
      481 tcaaccgcgg gacccctgac gaggtccgcg cccttcgtgg agtcgacctg acggtcgagc
      541 agggaagctt cgtcgttgtg atcgggacca acggctcagg caagagcacg actctaaact
      601 cggtcgccgg gtcgtttccc gtcgacgccg gcacgatccg gcttgccggc cgggaaatta
      661 cccgctggcc agagcaccgc cgggcgaagc tgatcggccg ggtctttcag gacccgctgg
      721 ccggcacttc gccgtcgctc tcgatcggag agaacctggc cctggcatcc cggcgcggcc
      781 ttccccgcgg cctcggccga gcggtgaccc ggcgcaaggt cgccgagttc cgcgaccggg
      841 tcaggccgct cggtatgggc ctggaagacc ggctcgacaa cccgatcggc agcctctccg
      901 ggggccagcg ccaggcgctg acactgctca tggccacgta ccgacgcccc gagctcctct
      961 tgctcgacga gcacaccgcc gccctcgacc ccaagagcgc cgaacaggtc atccggctca
     1021 ccgaccagat cgtccgccgc cagcaccaga cgaccctgat ggtcacccac tcgatggccc
     1081 acgctgcttc gctcggagac cgactgatca tggcccacca gggccgcctg accttcgatg
     1141 tctcaggcgc cgcgaaactc cgcctccgtc cccacgacct cctcgagaaa ttcgaggagc
     1201 tccgccgctc cgaccagctc gatgagaccg cagcccgtct tcttcgcgac gtctatgtct
     1261 gaaccgcctg cccttgccga tgaagcagcg tatcgagccg ggcggctatt acgcgatcgc
     1321 cgcgcgcgca gcgcggctgc cggtggcgga ggtctatcgg tggaccctcc gtgagccctt
     1381 gccccaccta ccgatcccgc ttcgcgagcc cgatccggat cttctgatcg atctcgccac
     1441 attggtcagc cgcgtccagc aacggagccg ctacgagcgt acgctccgct ccgccacgcc
     1501 aggcccatcc gcgaaacgac atcgttaacg cccgaggccc cagcctcggt tgagagtcag
     1561 tgagctgttt cgccgagcga tgtgagcttg taagtcaggc gcagaaatgg acacccgagc
     1621 caacgtcagt cgccgacact atctcggtca aacttaatgg agatgaaggg gatcgaaccg
     1681 acaacgtcct gattgcaaac ctggcgtaca ttcgatcgta aactacttgt ggataagatt
     1741 ttagatgtga ttgtcggccg ctcggtcgtt cccagcgttt tccaagattt tcgagcaatt
     1801 cctacgtgac ggagaaggaa ccttgtcagg ctgagctcaa attcattggc agcatgaccg
     1861 gcgacaatct gcaggtcttg gaagtttgct tgatctatgg cacccgcgcc ggtgaagagt
     1921 cggaaaattc gctggtcatc cgttcgattg gcgatttcgg tcagtcgatc gtcgcagctt
     1981 ggaatttggt gcgtgaggga ttgattaatc cggtcaagcg agagcttcgc ttcgtcatcg
     2041 aatccactgt taaatacctt tacgtagacc agcagatccg gaacggggcg acaattccca
     2101 agcttccgga caggctagat tccctcttcg caagatcggc cgcctgatgt tccgggtcta
     2161 caacatcgcc ctgacgctgt ttttccacgg ctatcatttg ttgatgacga gggatgtgtt
     2221 tatcaactaa aatcgtttcc cggcatcgac aaccagtctc gggaccagat tcggtgagca
     2281 tcgtgcgggg cggcaacgcc gtactcgccg cagggccaaa aagggacgtc acgcgcgtca
     2341 agccgatcaa cgcgtcgacc accgtcctcc ggaacacggt cgacacgaac aaagcctacc
     2401 acgccgcgct ccagcccaac tcgatctggc agttctatca gctcgtcatg acccaatggc
     2461 ccctcacgcc gaacagcccg acgacgcccg gagacccggc ccacacattt ccggggagtc
     2521 ctcccaacga ccagtctgcc ttttcgaatg ttacgctaga gaccttcgac caggggagta
     2581 tcttcaccgg ctgcatggcc tgtcacaacg cgacgatgca acccacggat ttcctctggt
     2641 ctctgaacga c
//
LOCUS       DATAQL010000582         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5097202, whole genome shotgun sequence.
ACCESSION   DATAQL010000582 DATAQL010000000
VERSION     DATAQL010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5097202"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..613)
                     /locus_tag="VHS97_10125"
     CDS             complement(158..613)
                     /locus_tag="VHS97_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448599.1"
                     /translation="MGHWGVKSYEHDEANDALDAGFERVHGARYEELMDDSNPLTFDQ
                     VQATLADSRTLLASIDSLRDSLALDVVPTNWDELARLALVGIVVRHAELGVPIPEPWG
                     HHAIDWLEHEEIEWDETTARRLRRGKEIAIVRKALRAGGSDQPAVVPLI"
     gene            877..1194
                     /locus_tag="VHS97_10130"
     CDS             877..1194
                     /locus_tag="VHS97_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448600.1"
                     /translation="MTLPSLLEEPGRVDEPQAAQAYADVLSQVAVGGRPVIVCRNGTD
                     LVAVIPLKHLELVREILTNLEVERSAASIKCGVTPNALQPPQSWFDDEDDHPFEPEEA
                     PAS"
     gene            1191..1607
                     /locus_tag="VHS97_10135"
     CDS             1191..1607
                     /locus_tag="VHS97_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX3448601.1"
                     /translation="MSPTRQPRLGSVVWAEVEDVNGFVKVRPVAIVTPTAEIAPGKTV
                     RVVAITTRLSSPLPDDHVLLPWDPQGKSRSGLRRRCAAVASWQVEISFANVQQVVGIL
                     PPTVIAELLAKIAATLPPPSASPVSGLGDPENQPSL"
BASE COUNT          330 a          571 c          535 g          333 t
ORIGIN      
        1 aatgcggcga ccgcaatggc cgcgaccgcc aacaacgagg ttcgagcgcg accgcgatcg
       61 aacacgcgga agatgtgact catgattcgc gtccttcaag ttactttcgt gacacgaggt
      121 acacaaaaga acgaataggt cgcgaatggg ggcgctgtca aatcaagggg acaaccgctg
      181 gctgatcgct gccgccggct ctgagcgcct ttcgaacgat cgcgatttcc ttcccgcgcc
      241 gcagtcggcg ggccgtcgtc tcgtcccact caatctcctc gtgttccagc cagtcgatgg
      301 cgtgatgccc ccacggctcg ggtatcggaa cacccagctc ggcgtgccgc accacgatcc
      361 cgacgagagc caaccgagcc agttcgtccc agttcgtcgg taccacgtca agagccagcg
      421 agtcgcgcag cgaatcgatc gaggcaagaa gtgtccggct atccgccagg gtcgcctgca
      481 cttgatcgaa ggtcagcggg ttagagtcgt ccatcagctc ttcgtaccgc gcaccgtgta
      541 cccgctcgaa ccccgcgtcg agcgcgtcgt tggcctcgtc gtgctcgtag ctcttaacgc
      601 cccagtggcc cattttggca atattgcttc gtggttccag aagccgtgtg tgtccaatcg
      661 gcctttagcg ctctccactc atcgcgtttc cgccccgcgc tggcagtgcg ttcgagagga
      721 ttggacgttg cgtccaagcc tctcctctgc ttggatacct gtcaataatc tcccccgtga
      781 acgcaccgaa atgtgcccgc ttctgctata atcggtgtcg accatgctgc aacggccacg
      841 gcgaagggcc gtcgaagtcc atgggaggag tgaaacatga ccttgccgtc cttgctggaa
      901 gagccaggcc gcgtggatga accgcaagcg gcccaggcat atgcggatgt gctcagccag
      961 gtcgcggttg ggggccggcc ggtgatcgtc tgtcggaacg gaacagacct tgtggcggtg
     1021 atcccactaa agcacctgga gttggtccgg gaaatcctca ccaacctgga agttgaaagg
     1081 tccgccgcaa gcattaagtg cggcgtgacg ccgaacgcgc tccagccacc tcagtcctgg
     1141 ttcgacgacg aagacgacca cccgttcgag cctgaggagg cgccggcgtc gtgagcccca
     1201 cccgccagcc gcgactcggc agcgtcgtat gggctgaggt ggaggacgtc aacggcttcg
     1261 tcaaggttcg cccggtcgcc atcgtaacac ccacggcgga gatcgctccg gggaaaaccg
     1321 tgcgcgtagt agcgatcacg acccgcctct cgagccctct tcctgatgac catgtactgc
     1381 tgccctggga cccgcaaggg aagtcgagat cgggcctgcg gcgccggtgc gcggccgtcg
     1441 caagctggca ggtggagatc tctttcgcga atgtgcaaca ggtggttggc atcctgccac
     1501 cgacggtgat cgccgagctg ctggcgaaga tcgcggccac tttgcctccg ccgtcggcat
     1561 ctccggtttc tggacttggc gaccccgaga accagccttc cctttgaata cgccaaagcg
     1621 ttccgccaca tcgagccggc gacgtcgcga gccgttgccg atcacgtcgc caagcacgca
     1681 gtgcacctga aggagggctt gcgcactctc ggcacgccgg cgtcagggca attcgatcgt
     1741 tgtcagtttt ctgaaccacg cgttcagat
//
LOCUS       DATAQL010000583         6780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1128083, whole genome shotgun sequence.
ACCESSION   DATAQL010000583 DATAQL010000000
VERSION     DATAQL010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128083"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..350)
                     /locus_tag="VHS97_10140"
     CDS             complement(225..350)
                     /locus_tag="VHS97_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448602.1"
                     /translation="MGFLTTRRDFDHFAKMLTQTALGQAEDTLSALLRKAAAKEE"
     gene            650..1420
                     /locus_tag="VHS97_10145"
     CDS             650..1420
                     /locus_tag="VHS97_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448603.1"
                     /translation="MRIRARLLLGLYGLLLAVPTIAAAAPLGDDQAGYATAGGQPPPQ
                     PAPHHHHKGLLGRRHCVECQRAYVKAHDGVDVPAPPPIAAGAMMHGQVVVAQPGACDV
                     CQGSGVVSGPVTMVNASAPGYAVVDGPGMMANANAPGYAVVGDIASGPEPSPIGVSRA
                     GQDPRMAAMGPRPGAGAYDPSVVPTSIPPAQVALSGPGHDRPHVISHLLGIPKMGTIR
                     RKIEDKERQKHAAIAYDQPGGKVTDLPASMVYGDKNGH"
     gene            1966..2790
                     /locus_tag="VHS97_10150"
     CDS             1966..2790
                     /locus_tag="VHS97_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448604.1"
                     /translation="MPVALALATTLGLGLGLASLARADGLLFQHTIPREVDAYDFTTG
                     GPFKAPPVPYGHYAKDYVADAQKAAGCITCRLHGLFGGPGAGHGLLHKGGHDGDGFGH
                     GDGAVVDDGTGSGHGAGHGLFGHHATLPYDGYGSSESAGFATTWAQASGQGVVQPSGH
                     SICGQSGCQVASKHSHLCGDAGCGKGHGHGLGHGGGTGCGLCGGRGCSSCLSGLRSGL
                     QSKLASLTGAFHRPKISWFLGAGGPVPITPGYVPYIVTTRSPRDFFSFPPMNPNDP"
     gene            2996..3571
                     /locus_tag="VHS97_10155"
     CDS             2996..3571
                     /locus_tag="VHS97_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448605.1"
                     /translation="MKCPLGLRGRKWIEEGRQGTAGHVPRLAGFAVVGLWFWLASAPA
                     SAQPPQPFVPDITERSGLMMRFAATPELLPPDPHRDFFYETRYADSGRVTHPNWFKTQ
                     GLYGLGMKTPDTESVYPYFYGMPGQSTVDSSSRPWWRPARFFQGLAQPWRPVGMYYQF
                     GSYVPIYDLDPIAPGPGPYPYPFYFNWVHGG"
     gene            complement(3782..5698)
                     /gene="htpG"
                     /locus_tag="VHS97_10160"
     CDS             complement(3782..5698)
                     /gene="htpG"
                     /locus_tag="VHS97_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX3448606.1"
                     /translation="MATVTDRQEYSFQAEIKQVLHLLSHSLYQSREIALRELISNASD
                     ALDKMRYISLTDDAQRDSGPLEIVLEGREAENELVIHDTGLGMAHDELVANLGTIAHS
                     GSGEFLRALASAKQEKDKAELSLIGQFGVGFYAAFMIADRVRVRTRSYAEQSGWEWES
                     EGTGTFTITPVEEALPRGTEVILHLKEDAKDFASPWRIKEIVRRYSSFVPYPIRLGAD
                     GEVINDQKPIWVEPKNQVTEEQYIRFYQHLAHHPEEKPLWHLHMAADSPIQFRAVVYC
                     PPTNLENLGFARIDHGLNLCAKRVLVQSDCKDLVPEYLRFLRGLVDSEDLPLNVSRET
                     LQDNSVIRKIRSSLVKGVLDRLDHLAQDDADKFSLFYDQFGRTLKEGLVVDHANKERI
                     AKLLRFPSSKSDDPKVRVSFDEYVARMSAGQERIYYLGGADLSSIAKSPNLEIFRRKG
                     LEVLYLTDPIDEFALTSLRNYQGKELTSIDSADLDLPETATTSASDIKTDDSPAKESG
                     FSRVLDLFRAALGNRVSEVRESKRLTDSPCCLVNAEGGMSTQMQRLLKMANKDFHESA
                     RILEINPSAALVRRLCRLSANQQHDDFIKNCALQLWTNAMILDGLTPEPEDMVARIQT
                     FMAEAAEKRSPLII"
     gene            6170..6451
                     /locus_tag="VHS97_10165"
     CDS             6170..6451
                     /locus_tag="VHS97_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3448607.1"
                     /translation="MSARETLRTTLLSLLEEEMGEKYPVLQDDQDLRETLGLDSVDVV
                     GLVMRIEREFRVRLSPEELANVKVVGDLLGLMETKLAERPDVPVNGEED"
     gene            6614..>6780
                     /locus_tag="VHS97_10170"
     CDS             6614..>6780
                     /locus_tag="VHS97_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3448608.1"
                     /translation="MAAIRNLLATWTTTGGKKPAAQSLFEGHTDVVRCLAFTPDGALL
                     ASGSSDGTVRL"
BASE COUNT         1279 a         1997 c         2079 g         1425 t
ORIGIN      
        1 cggagcagaa acgtcacctg cctcgaagac cgtctgctat caaatgagtt tttttctcgt
       61 ccacgcgtcc caggttcccg catcgttccg cgccggcctg gccgcgccaa ctggggttca
      121 tctttcacac ctctttgtcg cgtttacggc cgagtggcaa gtctcaacaa tagatcttgc
      181 aagtttcgat cgagtgtcaa ttcggggctg acgcctgaca acggctactc ctccttcgcg
      241 gctgcctttc gcaacaaggc ggagagcgtg tcttcggcct ggccaagggc ggtctgcgtg
      301 agcatcttgg cgaaatgatc gaagtccctg cgagtggtca aaaaacccat gtttttccat
      361 actaatcgca aggccagccc tgtttgtgcc gataaaacgg aatgtacgag aaggccgtgc
      421 ccccgttgag agggaagtcc gtcgttgcgt ttctgacgtg gcgctcggcc atccctaccg
      481 ccggagcgtc gaccttcgga cggagggctg cccccctcac tctcggccgg tgcggcaatc
      541 gacagttccc acggtcgagc cttcttagaa cgcttcggtt cgatcggtcc aggcgcgtgc
      601 atgttgtatg gcgcggcgcc gggcgtgaac ctgggaaagg agttccctca tgcgtatccg
      661 tgctcgtttg cttctgggac tatacggcct gctgttggcc gttcccacga ttgcggccgc
      721 ggccccactt ggtgatgatc aggcgggcta cgcgacggcc ggtgggcaac cgccgcccca
      781 gcccgcgccc caccatcatc acaagggatt gctcggacgg cggcactgcg tcgagtgcca
      841 gcgcgcttac gtcaaggccc acgacggcgt ggatgttcca gctcccccgc cgatcgcggc
      901 cggtgcaatg atgcatggtc aggttgttgt tgcccaacct ggagcgtgtg acgtttgcca
      961 ggggagcgga gttgtgtcgg gaccggtcac gatggtcaac gcgtccgctc cgggctacgc
     1021 ggtggtcgac ggcccaggca tgatggccaa cgccaatgca ccgggctatg ccgttgtagg
     1081 tgacatcgcg tcaggtcccg agccgagccc gatcggtgtc tcgagagccg gtcaggatcc
     1141 gcggatggct gccatgggac ctcgtccagg cgctggcgcc tatgatccct cggttgtccc
     1201 cacctccatt cctccggctc aggtcgcgct gtctggccct gggcatgatc gcccccacgt
     1261 gatcagccat ttgctcggca ttcccaaaat gggaacaata aggcgcaaga tcgaggacaa
     1321 ggaacgccag aaacacgcgg cgatcgccta cgaccaaccc ggtggaaagg taaccgatct
     1381 gccggcctcg atggtctatg gcgacaagaa cggccattga tcgaaatcca aaagtcgcgc
     1441 cagggtaccg accgagtgga aggtgtgcac gaccctgttg atggttgaac gacacgtgga
     1501 acacgcgcca cgtgcagtga tgggagccga cgcggtccag gacgcgtcga ctcaacctta
     1561 cgtcaacctt gatggggaaa ctggaccggt ctctcggact gtaacgagag accggtcttt
     1621 tttttatggc cccgccccgc gattggtttg agacgggggt cgtttcggac cgatccagag
     1681 acacaaggct tattcacggg ccgaggccga aaccgcgcct cgggcaccat cggtcggcgg
     1741 ggcatgacgc cggaagaggg ccaggatccc tgtggggtcg gccggcttgc cgcggccaat
     1801 gtcgcctctg gaccgctgtg gtttgtgatg gcccggggct gatcgtctcg tccgcgcaca
     1861 ggaaggctcg ggcacgtctc tcgattgctc gattccagac aatccaggag ggtcttgtgc
     1921 aagcaaaata agggatagac catgcttttt ggaaaatggc gagggatgcc agtcgcgctg
     1981 gcactggcga ccaccctcgg gctgggtctg ggattggcga gcttggcccg ggcggatggc
     2041 ctgttgtttc agcacacgat tccacgagaa gtggatgcgt acgacttcac taccggcggg
     2101 ccctttaagg caccaccggt tccatacggc cactatgcca aggactacgt ggccgacgcc
     2161 caaaaggccg cgggatgtat tacctgccgg ttacacgggc tcttcggcgg tcccggcgcg
     2221 ggacacggtc tcttgcacaa gggaggccat gacggcgacg gctttggtca tggcgatggt
     2281 gctgtcgtgg acgatgggac cggcagcggg cacggtgcgg gccacggatt gttcggccat
     2341 cacgcgacat tgccgtacga cggctatggt agttcggaat ccgcgggttt cgccaccaca
     2401 tgggcccagg cgagtggtca gggcgtcgtt cagccgtccg gccattcaat ctgcggacaa
     2461 tccggctgcc aggttgcgtc aaagcattct cacctttgcg gcgacgccgg gtgcggcaag
     2521 ggtcatggtc atggtcttgg tcatggtggt ggaacaggtt gcggattgtg tggaggcagg
     2581 ggttgctcaa gctgcctgtc gggactcaga tccggccttc agagcaagct cgcctcgctg
     2641 accggtgcct tccacaggcc caagatttcg tggtttctgg gtgcgggcgg acccgttccg
     2701 atcacccctg gctatgttcc ctatatcgtg accacccgtt cgccgcgcga tttcttttcc
     2761 tttccgccca tgaatcccaa cgacccctga gtggaggcaa tgaaacgcgg cgggccctgt
     2821 cggacctgat gcctttcgcg ggatgggccg acagccgacg cagttgagga tcaatgggga
     2881 gtttcatgac aaactctccg cccagaacgc ggaaaaaggg cccggccgga gcgccgggcc
     2941 cggttccgct ggcgacttgc gggtattgtc caaaccgagc tgaggagacg agctcatgaa
     3001 atgcccatta ggtctgcgtg gacggaaatg gattgaagaa ggccggcagg gcacagccgg
     3061 ccacgtcccg aggctggctg gctttgccgt cgtgggcctt tggttttggc tggcatccgc
     3121 gccagcttcg gcccagccac ctcagccctt cgttcccgac ataacggaac gaagcgggct
     3181 catgatgcga ttcgcggcga ctcccgagtt attgcccccc gacccacatc gcgacttctt
     3241 ttacgagacg cgctacgccg acagcggcag ggtcacgcac cccaattggt tcaaaaccca
     3301 gggcttgtac gggctgggaa tgaagactcc cgacacggaa agtgtctacc cctatttcta
     3361 cgggatgccc ggccagagca cggtggactc atcgagccgc ccgtggtggc ggccagcccg
     3421 attcttccag ggtttggccc aaccgtggcg ccccgtcggg atgtattacc aattcggatc
     3481 gtacgtcccg atctatgacc ttgaccccat cgcccccggc cctggtcctt acccataccc
     3541 gttctatttc aattgggtgc acgggggcta aagcgcgcta aaccgagact tgacgcagcg
     3601 actgtgcttc ttcgtaaggc aatgggttag tgttgcgcca ggccgagcca caatgccatc
     3661 gaactaagag aagtagtggc tcatatcaat ttgtaatccc ctccccccat gtgggggagg
     3721 gttagggtgg gggcgtttca cttccgagct cagcgcccaa ccgcttgatt ttccagtcgg
     3781 ttcagatgat gagcggcgaa cgcttctcgg cggcctcggc catgaaggtc tgaatccgcg
     3841 cgaccatgtc ctcaggttcg ggcgtcagcc cgtccaggat catcgcattc gtccagagct
     3901 ggagtgcaca gttcttgatg aaatcatcat gctgttgatt ggcgctcagc cggcagaggc
     3961 gtcgtaccaa cgcagccgac ggattgatct caaggattcg cgcactctcg tggaagtctt
     4021 tgttggccat cttcaagagc cgctgcatct gggtgctcat gcccccttcg gcgttgacga
     4081 ggcagcatgg gctatccgtc agacgcttcg actcgcgaac ctcgctgact cgattgccga
     4141 gcgcggcccg gaacaggtcc aacactcgac tgaagccgct ttccttggct gggctgtcgt
     4201 cggtcttgat atccgaagcc gaagtcgtcg cggtctcggg aagatcgagg tcggccgagt
     4261 cgatcgaggt cagctctttc ccctgataat ttcgcagcga tgtgagggcg aattcgtcga
     4321 tcggatcggt gaggtacaaa acttccagcc ctttgcgccg gaaaatctcg agattggggc
     4381 tcttggcgat cgacgagaga tcggcacccc ccagatagta aatgcgctcc tggccggcgg
     4441 acattctcgc gacatattca tcgaacgaca cgcgcacttt cgggtcgtcg ctctttgaag
     4501 acggaaatcg cagcagcttg gcgattcgct ctttgttggc gtggtctacc accaatccct
     4561 ctttgagcgt cctgccaaac tggtcgtaaa acaggctgaa cttgtcggca tcgtcctgag
     4621 cgaggtgatc caaacggtcg agcactccct tgaccagcga cgagcgaatc ttgcggatga
     4681 cgctattatc ctggagcgtc tcgcgagaga cgttcaaggg caagtcctcc gaatcaacca
     4741 atccgcggag gaaccgcagg tactcgggca ccaagtcctt gcaatcgctt tgcaccaata
     4801 cgcgcttggc gcacaggttc agtccgtgat cgatccgggc gaatcccagg ttttcgagat
     4861 ttgttggcgg gcaatagacg actgcccgga actggatggg cgagtcggcc gccatgtgca
     4921 ggtgccagag cggtttttct tcgggatggt gagccagatg ctggtaaaac cggatgtatt
     4981 gctcttcggt gacctgattc ttgggctcga cccagatcgg cttttgatcg ttgataacct
     5041 cgccatccgc ccccagtcgg atcggatagg gaacgaaact cgaatagcgc ctgacgatct
     5101 ctttgatccg ccatggcgag gcgaaatcct tggcgtcttc cttgagatga aggatcactt
     5161 cggtgccgcg cggcagggcc tcttcgaccg gcgtgatggt aaaggtcccg gtcccttccg
     5221 actcccattc ccagccggac tgctcagcgt agctgcgcgt ccgcacccgc actcgatcgg
     5281 cgatcatgaa ggccgcgtaa aatccgacgc cgaactggcc gatcagcgat agctcggcct
     5341 tgtctttttc ttgctttgcc gacgccagcg ccctgagaaa ctcgccggaa ccgctgtgtg
     5401 cgatcgttcc gaggttggcc accagctcgt cgtgagccat tccgagaccc gtgtcgtgga
     5461 tgaccagctc gttctcggcc tcgcgcccct cgagaacgat ctcgagcgga ccactatcgc
     5521 gctgggcatc gtcggtcagg gagatgtatc tcatcttgtc gagcgcgtcg gacgcgttgg
     5581 agataagctc gcgcagcgcg atctcccggc tctgatacag tgagtgagac agcaggtgaa
     5641 gcacctgctt gatttcggcc tgaaagctgt attcctggcg atcggtaacg gttgccatag
     5701 cattgaccct acgaagatat gtcttgtctg aaacggccgc cggacgtcgc ggcttggcgc
     5761 agggaaacgc cgcctgattt caccgaacta cgacttcatt ttagcaagcc gcgtaccagc
     5821 acaaacacta tcctcaagat cttgatacgt aaagagttgt tctcgaattt gggccgaata
     5881 aaattgtcgc tcgcctgcca ttacgggcgt ccgcatgaca atttggcaag ccgggatagt
     5941 gcatggcgcg gccagcgatt gataaatggc gagccgttct tcgcaacgac tcgtcagtcg
     6001 gcacctccgt tcgtttgaac ttgccattta tacgcgaccg gacgacgaaa tgcccaaagt
     6061 ggtcacggct gtgggacttt cgtctggtga aagcgtgggt tgcgctgtta tcttcgacgg
     6121 attgagagac aaatatcgcc gtacaaccag ccatcaggac ttgcgacgaa tgtcagccag
     6181 ggagacgctt cgaacgacgc tcttgagcct gctcgaagag gagatgggtg agaagtatcc
     6241 ggttctacag gacgatcaag acttgcgcga aaccctgggc ctcgattcgg tggatgtggt
     6301 cgggctggtg atgaggatcg agcgcgaatt tcgggttcgg ctctcgcccg aggaactagc
     6361 caatgtcaag gtcgttggcg acctcctcgg cctgatggaa actaaattgg ccgagcgccc
     6421 cgacgttccg gtgaacggcg aagaagatta agattgatca cctctgggct ttgttgattg
     6481 cgctctggcg gtccgggcga ggcggccaag acattcttgt ttggtggcgc ccgtccgatg
     6541 tacaaatcag ttgccgccgt tttgagacag cgccgaaagt gtaccctgcg gcagcatcga
     6601 tctggaggtc gctgtggcgg cgatccgaaa cctccttgcc acttggacaa ccaccggggg
     6661 caaaaagccc gcggcacaat ccctcttcga gggacatacg gatgtggtgc gctgtctggc
     6721 ctttaccccg gacggcgcgc tgctggcttc gggcagttcc gatgggacgg tgaggctgtg
//
LOCUS       DATAQL010000584         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3266969, whole genome shotgun sequence.
ACCESSION   DATAQL010000584 DATAQL010000000
VERSION     DATAQL010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3266969"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2971
                     /locus_tag="VHS97_10175"
     CDS             <1..2971
                     /locus_tag="VHS97_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448609.1"
                     /translation="QTPEHLVRDLLVVAGSVGAAATSSPLPSWLDEAHRHAWERHLVW
                     LVPVLSLFLVVTGLGWWGREPSKQSLSAQRSIGPTVALRPGDANALAGAAALGSSPSS
                     VTGEAAPPASGAVPAYLRNIPVSSNEDLLEILATAPRRSVIVLADDGPYRLGGRTRSS
                     RSTAPLANHDLTIKAEPGVRPIIKFATDARLADRPQKSLLRFVAGHVTIEGLEFELDA
                     VLPDELVTAVLTEDTELTVRGCSFRRTNSREGRNVAAIQVRSVRSLAAGDRPPAVVAD
                     SCHFDGGQTGILAEGPVDIVLRDCTMGPGQPSIWFDNVRSNVPVYSELRLKHASIMAG
                     SEPVFRFDGTQTRVWVEDSVVAPAGRSPATLVMVDNARNLLWRGRSNLYSRIGVYLGI
                     STRDDRQESISDFARWSETPTEVRETGTALTANSVWETTDPGQALLAEADNPTGVFLM
                     SAASTSRSDLGARQGPFGSDLKNARIAEKRSRPDESVVPPARSTVREQVGRQATEPAD
                     AKMPTTVAGEPRIPDSVGPTPITPAPTADTQVASSSDDPMDLPTMPPMSTAGQPTEEP
                     AATTGTGNGGATSAGHSSTAGSATAARDAGDPARNDRERRPAFEDEDVIRSSEQFITM
                     FNRLGRQGGKLRIAAGADLDLTSIMIDGTGLYQITAEPGSAARRPRFRFRASADTRKS
                     PADWTVMLNLQSGSLHVQGIDFVVSDQDMARADRLAVAGVVPGTELTMTDCTLTLAAN
                     RPGAALVVVQPIFAARNSPVADVGDVSSAIIRLRDCFLRSGGEGITVAAGRKVDVHFT
                     NVLVSTEGSLLRAVGGGRSGRADSPALKVRLDQVSALVRGGLVHLDSTGTAGGPELPF
                     TAIEAENSIISTSGRDVPLFRLDGRDQRDDFIDKIHWSGRKVAYDRIQTYRRDEVFQI
                     GGSPKIYNRANWTTAFSPTDESPMLGEVKFLREIDPSRSAWKIVRDDFLLAPISPNAD
                     RGPDLVPIPAAPEEGDL"
     gene            complement(3024..4238)
                     /locus_tag="VHS97_10180"
     CDS             complement(3024..4238)
                     /locus_tag="VHS97_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX3448610.1"
                     /translation="MEDSVGENQGTVAPKGDAPAGGTFVSRLTAQGRGAIAVVRVCGP
                     RAIEIADAVFRQNRGVRLAETPRGVLRLGHIGQGLGDEVVAVVLEEELPTVEVQCHGG
                     EAAVSLVVEALQAAGANWFDAVSFGDQCWDDPFAHDALVDLARAPTLLTAEILLDQVN
                     GALRRALHRLSPSIDEEPDRVLADIETLIRRSEVGLRLLNGWRVVIAGRPNVGKSRLF
                     NALVGFARAIVDPTPGTTRDLVSFKTSFAGWPVELVDTAGLRETLDPIETIGIEHSRL
                     EQRQSDLILLVLDRSLPLELIDLELVASNPTALLVGNKSDLNPGWNDAELSAGLHNMV
                     FVSAETGEGLTDLIAAIVARLIALPQLPGAAVPFRAAHVSALLEIRASLIAGDRAAAA
                     GRLALMFDGPRC"
     gene            complement(4311..5126)
                     /locus_tag="VHS97_10185"
     CDS             complement(4311..5126)
                     /locus_tag="VHS97_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448611.1"
                     /translation="MHAQDPHDRNGRCPWRRRRFRPACERLESLALLSSLGPNLPGKH
                     FPAPNVQQFVPLLYPPGTPQPTAAEVQRESFVAKGTGRYTIGPGQFDTQTITIHGFGK
                     PMTSNISRKLHFQFVVFEPTDPSQAINGNINLVGGNYLQNSTDLILALEGPTSSESNG
                     LPTHLFFVTDSNSPSATAFAETGGALPAFSNYPANYFTSSGNLAPPPGSPGSLGPPTS
                     VSNWGMALGDANFKYIPDKHPQPGSLGSGTVIVELTGLRNYSGAQSQFDKQIN"
BASE COUNT          951 a         1634 c         1659 g          953 t
ORIGIN      
        1 ccagacaccc gagcacctgg ttcgcgatct tctcgttgtg gccgggtcgg tgggcgcggc
       61 ggcgacttca agtcctttgc cgtcctggct cgatgaagcg catcgacacg cgtgggaacg
      121 tcatctggtc tggttggtgc cggtattatc gttgtttctg gtggtgactg ggcttggctg
      181 gtgggggcgg gagccttcga agcagtctct gtcggctcaa cgctcaattg gcccgaccgt
      241 ggcgctgcgc cccggcgacg ccaacgctct ggccggcgcg gccgcgcttg gctcttcgcc
      301 gtcgtcagtc acgggtgagg ccgcaccccc agccagtggc gccgtgccgg cctatctccg
      361 caatatcccg gtcagctcga atgaagatct gctcgagatt ctggcgacag cgccgcgacg
      421 gtcggtgatc gtgctcgccg acgatgggcc ttatcgtcta ggaggaagga cacggtcgtc
      481 gcgctcgacg gctcctctgg cgaatcacga cctgacgatc aaggcagagc cgggtgtccg
      541 gcctatcatc aagtttgcga ccgatgcacg cctggcggac cgtccgcaga agagtctgct
      601 ccgtttcgtc gccggacatg tgacgatcga gggcctggaa tttgaattgg acgcggtgtt
      661 gccggacgag ctcgtgacgg ccgttttaac cgaggacacc gagctcacgg tccggggctg
      721 ttcattccgg cggaccaatt cgcgggaagg ccgcaacgtc gcggcaatcc aggttcgttc
      781 ggtccgctcg ctggcagcgg gcgatcgacc gccggccgta gtcgcggatt cgtgtcattt
      841 cgacggtggc cagaccggca tcctggcgga agggcccgtc gacatcgtct tgcgcgactg
      901 cacgatgggg cccgggcagc cgtcgatctg gtttgataac gtgcgatcga atgtcccggt
      961 atatagtgag ctgcggctca agcacgcgag catcatggct ggctcggaac ccgtcttccg
     1021 tttcgatggc acccagaccc gcgtctgggt tgaagacagt gtggttgccc cggcgggccg
     1081 atcgccggcg accctcgtca tggtcgacaa cgcgcgcaac ctcctttggc ggggccgctc
     1141 aaatctctat tcgcggattg gcgtctacct ggggatttcg accagggacg atcgccagga
     1201 atcaatcagc gacttcgcac gctggagcga aaccccaacc gaagtgcgcg agacagggac
     1261 tgccctgacg gcgaactccg tttgggaaac cactgaccct ggtcaagcgc ttttggccga
     1321 agcagacaat ccgacgggcg tatttctcat gagcgcggcc agcacgtccc ggtccgacct
     1381 gggcgctcgc cagggaccgt tcgggtcgga cttgaagaat gcgcgaatcg cggagaagcg
     1441 atccaggccg gacgagtcgg tcgtcccgcc ggcgcgaagc accgtccggg aacaggtggg
     1501 tcggcaggca accgagcctg cggacgccaa aatgccgacg acagtcgccg gcgagccccg
     1561 catccccgac tctgtcgggc cgacgccgat cactccggca cctacggccg acactcaggt
     1621 cgcttcgtcc tctgatgatc cgatggacct tcccacgatg ccaccgatgt cgaccgccgg
     1681 gcagccgacg gaagaaccgg cggcgacgac aggaactggc aacgggggtg cgacatcggc
     1741 cggccattcg tccacggccg gcagcgccac agccgcgcgg gatgcaggcg atcccgcgcg
     1801 caacgaccgt gagcgacgtc cagccttcga agacgaagac gtgatccgca gttccgagca
     1861 attcatcacg atgttcaatc ggctgggtcg tcagggcggg aagctccgta tcgccgcggg
     1921 agccgacctt gatcttacgt ccatcatgat cgacggtacg ggcctttatc agattacggc
     1981 cgaaccgggg tcggctgcac ggcgccctcg gtttcgattc cgcgcatcgg ccgatacccg
     2041 caaatccccc gcggactgga ccgtcatgct gaacctccaa tcgggatcgc tgcacgtcca
     2101 gggtatcgac ttcgttgtat ccgaccagga tatggcgcgc gccgatcgcc tcgccgtcgc
     2161 gggagtcgtg cccggtaccg agctcacgat gaccgactgt acgcttactc tggcggccaa
     2221 tcgaccgggc gccgcgcttg tagtggttca accgatcttc gccgctcgca actctcccgt
     2281 cgccgacgtc ggggatgtct caagcgcgat cattcgcctg cgtgactgct ttctccgctc
     2341 cggaggcgaa ggaatcaccg ttgccgcggg ccgtaaggtc gacgttcatt tcaccaatgt
     2401 gctggtcagc accgagggca gcttgctgcg cgcggtcggc ggagggcgct cgggtcgagc
     2461 ggattcgccc gcgctcaagg tccgtctcga tcaggtgtct gcgctcgtga gaggaggtct
     2521 ggttcacctc gacagcaccg gcacagccgg cggaccagag cttcccttca ccgcgatcga
     2581 ggctgaaaat tctataatca gcacgtccgg ccgcgacgtg cccctctttc gcctcgacgg
     2641 acgcgaccag cgagatgact tcatcgacaa gatccattgg tcaggacgca aggtcgccta
     2701 cgatcgaatc cagacctatc gacgtgacga agtgtttcaa atcgggggtt cgcccaagat
     2761 ctacaaccgg gccaactgga ccacggcttt ctcgcccacg gacgagtcac ccatgcttgg
     2821 cgaagtgaag tttctccggg aaatcgaccc ctctcggtct gcctggaaaa tcgtgcgcga
     2881 cgactttctg ctcgcaccca tcagtccgaa cgccgacaga ggccccgacc ttgtcccgat
     2941 cccggccgcc cccgaagaag gcgacctgta ggcggctggc gtgagctcat agagacgcgt
     3001 gcattcctcc aacagcatcg aggctagcac cgcggaccgt caaacatgag agccagccgg
     3061 cccgccgccg ccgctcgatc gccggcgatc aggctggcgc ggatttcgag taatgcgctc
     3121 acgtgagcgg cacgaaatgg caccgccgcg ccggggagct gtggcagggc gatcagccgc
     3181 gcgacgatcg cggcgatcaa gtccgtcaga ccttctcccg tttcggccga gacgaatacc
     3241 atgttatgga gaccagctga caattcggca tcgttccagc ctgggttgag gtccgactta
     3301 ttgccgacca ataatgctgt cgggtttgac gcaaccaatt cgagatcgat gagctccagc
     3361 ggcagcgacc ggtcgagaac caagaggatc aggtcggact gtcgctgctc gagacgagaa
     3421 tgctcgattc caatggtttc aatcgggtcg agtgtctcgc ggaggccggc tgtgtcgacc
     3481 agctcgaccg gccagccagc aaacgaagtc ttgaacgaca ccaagtcgcg cgtcgtgccc
     3541 ggcgtggggt ccacgattgc gcgagcaaac ccgaccagag cgttgaacag acgactcttg
     3601 cccacattgg ggcggccggc gatcacgact ctccacccgt tgaggagccg cagcccgacc
     3661 tcggaacggc ggatcagagt ttcgatatca gccagaacgc gatcaggctc ctcgtcgatc
     3721 gaagggctga gtcggtgaag ggctctgcgc agagcaccgt tcacttgatc gagcaagatc
     3781 tccgcggtca aaagcgttgg ggctcgggca aggtcgacca gggcatcatg ggcgaacggg
     3841 tcgtcccagc actggtcgcc gaacgagacc gcatcaaacc agttggctcc cgctgcctgg
     3901 agggcttcaa cgaccagaga aacggcggct tcgcctccgt ggcactggac ctcgactgtc
     3961 ggcagctctt cctcgagcac gacggcgacg acctcgtcac ccagtccttg gccgatgtga
     4021 cccaggcgta atactccgcg aggggtttcg gccagccgca cgccgcgatt ttgcctaaag
     4081 acagcgtcgg caatctcgat cgcgcgcggc ccgcagacgc ggaccactgc aatcgcgccg
     4141 cggccttgtg ccgttaggcg gctgacgaaa gtacctcccg caggcgcatc gcccttaggc
     4201 gccactgtgc cttgattctc gccgacgctg tcttccatcg cgcggtcgat ccctggtgtt
     4261 gccggctgcc acgtcccggc atcgtaagaa gcgtgcgtca ccattgtcca tcaattgatt
     4321 tgcttgtcga actggctctg ggcgccagag tagttgcgta aacccgtgag ttcaacgatc
     4381 acagtgcccg agccgaggga ccccggctgt gggtgtttgt caggaatgta cttgaagttc
     4441 gcgtccccca gagccatgcc ccagttagag acactcgtcg ggggtccgag gctccccggc
     4501 gaacccggcg gcggcgccag gttacctgac gacgtgaaat agttcgcagg gtagtttgag
     4561 aacgcaggga gcgctccccc cgtctcggca aacgcagtcg cgctgggcga gtttgaatcg
     4621 gtcacaaaaa acaggtgggt cggcaagccg ttggactcgc tgctggtcgg accttccagg
     4681 gccaaaatga gatcggtcga attttgcaga tagttgccgc caaccaggtt gatgttcccg
     4741 ttaattgcct gtgacgggtc cgtgggctca aagaccacga actggaagtg caactttcgg
     4801 gagatattgc tggtcattgg cttgccgaag ccgtggatgg ttatggtctg cgtgtcgaac
     4861 tgtccggggc cgatggtgta tcgaccagta cccttggcga cgaacgattc gcgctggacc
     4921 tcggcggcgg tcggctgcgg cgtgcccggc ggatagagaa gcggcacgaa ttgctggaca
     4981 ttaggtgcgg ggaaatgctt gcccgggagg tttggcccca gcgaggagag caaggccagc
     5041 gactcgagac gttcgcacgc aggtcggaac cgacggcgcc gccatgggca tcggccgttg
     5101 cgatcatgag ggtcttgtgc gtgcatcgag cacttctcct tccaggcgta accgctagga
     5161 aatgaccttc gcgatcgggt tgacgacccg gctgaga
//
LOCUS       DATAQL010000585         8496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3723781, whole genome shotgun sequence.
ACCESSION   DATAQL010000585 DATAQL010000000
VERSION     DATAQL010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3723781"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VHS97_10190"
     CDS             complement(<1..236)
                     /locus_tag="VHS97_10190"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX3448612.1"
                     /translation="MGLDPRELAPLLMQRVAVVAAEARPFQRAAIVMRRVGDEGVSAK
                     TIERVVHDVDPEMAERGDAVPRTDEGQGTVFGNRG"
     gene            441..1562
                     /locus_tag="VHS97_10195"
     CDS             441..1562
                     /locus_tag="VHS97_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3448613.1"
                     /translation="MRRNAPSPDLRPCTDGFAYTEDGWRLGVRHYRPEHPEPGKLPVI
                     LCHGLGLNATFWTITDNHLPAQLAARGYDVYVFDIRASGENAHLGRCDRINQHLRQTF
                     LKEHGERSWTVDDIVKYDLPAILDYVQRETGQDRVNWVGHSLGVMLIFPYLEFATRPE
                     RIANFVGMGSTIVQAKIPQTDMLRANQGLRLLTLVASPGRLGRPLAYLKVPGMEHIDR
                     FYYSNENVDQLTVSRFYGYTLEDTGAGALRQLAPYLMYGHMLSADRAVDYSAMLGRIT
                     TPTLLIAGDGDIMSDVPSTELTLAGLASPDKMMMRFGTSNGHVADYGHCDLVWSRYAP
                     REIFPPIIDWLDQRQPGAGPSPQSISPLHDGTISASPQN"
     gene            complement(1542..2153)
                     /locus_tag="VHS97_10200"
     CDS             complement(1542..2153)
                     /locus_tag="VHS97_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEX3448614.1"
                     /translation="MSQKVDERVLVVPSTELDRLGRFQGFSGEVDRYITALLVPELMQ
                     YRPRSEVENDPGYKQIIPYVVFRCNGSVFCYTRGKSQGEARLHRLRSLGVGGHVSEED
                     AQGGKDLDAYEAALRRELDEEVAVASPGRSGRVGLINDDSTPVGRVHLGVVHLFELEQ
                     PNVTAREEGLAEARFFPLSRVVTFRHEFETWSQICIESILRTS"
     gene            complement(2357..4156)
                     /locus_tag="VHS97_10205"
     CDS             complement(2357..4156)
                     /locus_tag="VHS97_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX3448615.1"
                     /translation="MRLIQVCVVIGSLWDVASTVRGDQPANNGNAVLESFFRDYLEAA
                     FRLEPMMATRLGEHRFDDQLDDLSQPARNAILNHDRAALAELGQKVNFANLSRDGQID
                     YGIFRQHLERNIWLSETFHPFEEDPRVYGSYITESVYLLLTQSSLPKEVNLKNALARM
                     AKIPRAIDIARSTIKNPVRVKVETAIRQAEGAIGFYSSELFQLAGQKPNHDELSSKAE
                     RIVASLNRHVAYLKNEVLPHAVDEWRIGPSRYAKKLELELDAGLSADLVLAEAEREAA
                     RVESEMALVARQMWGTVFPGRPLPIDDPQPRRVMIQQVLDDVASHHGTAESLVADVQG
                     TVGEIKTFITTHKIISLIEPDRCRIVLMPEFLRGNSVAYLNPAPPLDIRGSSEYAISP
                     PPRDWSKERAKSYLREYNRSMLKILTVHEAYPGHYVQLEYSNRCPSLIRRVLSSGTFA
                     EGWAVYTEQMMLDQGFGQADLGLRLQQLKFYLRAVVNAILDHRMHAGTMSDAEAMDLL
                     VGRAFQTEGEAIGKIIRAKQTSCQLSTYFVGRTAFYRLRQQIQRALGDQFDLARYHEA
                     VLSHGTIPVKYLPELVNRSLAVPDQVTKKQAPS"
     gene            4569..5165
                     /locus_tag="VHS97_10210"
     CDS             4569..5165
                     /locus_tag="VHS97_10210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3448616.1"
                     /translation="MCQVVLMRPGATIYDEQNRVQGVLDIPLSERGKGEVARMAQKLS
                     RCINGSPLSALYCGPGENVIGSAEIVGKVLGIRPKRIDEFRNLDQGLWQGLQIDEIRR
                     RNTKLFRQWIDDPETICPPLGETVECAMGRIKAAFRPLLKRHHDEAIGLVVGEPLATM
                     VASYLKCEPRVHLDEHWSCGGFEWIEIAPLLLRDGSAS"
     gene            5254..6102
                     /gene="accD"
                     /locus_tag="VHS97_10215"
     CDS             5254..6102
                     /gene="accD"
                     /locus_tag="VHS97_10215"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX3448617.1"
                     /translation="MAARDGKAKNAWHGHFEPKRVPEGIWMPCDGCQATLVRKQVEQN
                     DGICPECNHHFSLTAAERIGQLLDPDTFEEWFPGLQTLDPLGFNDRRPYPERIEAEQA
                     RTGLAEAAVVGQGFIKGIRIVIGITDSSFIMGSMGSVVGEKLTRAIEEATRQKLPLVI
                     VSGSGGGARMHEGIFSLMQMAKVSTALGRYHAAGGIYLSILTHPTMGGVAASFASLGD
                     FILAEPKALIGFAGPRVIEQTVRVQLPTGFQTSEFLCEHGFIDRIVNRRDLRSTIAQL
                     IDYAMP"
     gene            complement(6138..6896)
                     /locus_tag="VHS97_10220"
     CDS             complement(6138..6896)
                     /locus_tag="VHS97_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3448618.1"
                     /translation="MPKPIRVLALHAHPDDVEFQCAGTLTLLRHAGVAVTIATMTPGD
                     CGSAEYDAESISAIRREEARKAAELIGADYICLEFRDLAIFNDDESRRRITEAVRRVA
                     PALILTAPPADYIADHEMTSLLVRDACFAAPCPNYQTRQWEPAPALETIPHLYYVDPL
                     EGVDRDGRPVPVDFHVDISPVFATKREMLACHASQRNWLMRQHGIDEYLDIQAKWGAR
                     RGAEIGVPYAEAFRQYKGHPYPQDNLLLELLRHD"
     gene            7170..7775
                     /gene="lexA"
                     /locus_tag="VHS97_10225"
     CDS             7170..7775
                     /gene="lexA"
                     /locus_tag="VHS97_10225"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX3448619.1"
                     /translation="MADLDALTPRQKEIYNFIRSKIQGRGYGPTVREIGLHFQIKSPN
                     GVMCHLKALQKKGLIHREPNMSRAIQLLDDPVSAHQMGLKLVGRIAAGQPIEAVEQHE
                     ELTFSDWTEPGDKFALRVAGDSMIEEHIADGDFVIIKRQEQARDGQIVAVRDDDGEAT
                     LKKFFRDRNRVRLEPANKTMKPIFRDRVNVLGVLVGVVRKY"
     gene            7920..8426
                     /gene="rnhA"
                     /locus_tag="VHS97_10230"
     CDS             7920..8426
                     /gene="rnhA"
                     /locus_tag="VHS97_10230"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX3448620.1"
                     /translation="MGKDSCENGEVVRLFTDGACSGNPGPGGWAFILQHPASGKSRDS
                     SGAEPVTTNNRMELMGVIEGLASLKRPCEVELVTDSQYVAKGISEWLPNWKRRGWQRK
                     EKGQLKPVANVDLWQKVDSLMSVHKVWVTHVLGHRGHPENEACDRMAVDAYKKLVEEQ
                     KVQSQSRL"
BASE COUNT         1768 a         2467 c         2526 g         1735 t
ORIGIN      
        1 ccccgatttc cgaaaaccgt tccttgtccc tcatcagtcc tggggacagc gtcgccgcgc
       61 tcggccattt cggggtcgac gtcatgcacc acccgttcga tcgtcttggc ggagacccct
      121 tcatcgccta cccgtctcat gacgatcgcc gcccgctgga acgggcgtgc ctcggcggcg
      181 acaaccgcga cccgctgcat caagagcggg gccagttccc ttgggtcaag gcccaacgtc
      241 tcccggagcc gttttgattt ggagttccca acacgacgac cgttcgataa gatccgcggt
      301 aatcgtcaag cacggatgct tacggtcacg tcgaacggac gtgggaaggg attccaatcg
      361 aaatgcaccg tcgcaggcgg atccgattcg cctacgggtt gttgtgcacg gttttatgtc
      421 tggtggcggg atgtgccacc atgcgccgta atgcgcccag tccggatttg cggccatgta
      481 ccgacggatt cgcttatacc gaagacggct ggcgactcgg tgtccgtcat tatcggccgg
      541 aacatcccga accgggcaag cttccggtga tcctctgtca cggcctcggg ctcaatgcca
      601 cgttctggac gatcaccgac aaccatcttc ccgcgcagtt ggccgcccgt ggttacgacg
      661 tctatgtctt cgacatccgg gcctcgggcg aaaacgcaca cctggggcga tgtgaccgga
      721 tcaaccaaca cctccgccaa accttcctga aagaacacgg cgagcggagc tggacggtcg
      781 acgacatcgt gaaatacgac ctccccgcca ttctggacta tgtccagcgc gagacggggc
      841 aagatcgggt caactgggtc ggacatagcc tgggagtgat gcttattttc ccgtacctcg
      901 agttcgcaac tcgtccggag cggattgcga atttcgtggg gatggggagc acgatcgtcc
      961 aggcgaagat cccgcagacc gacatgcttc gcgccaatca agggctgcgg ctcttgacgc
     1021 tcgttgccag tccaggtcgt ctcggccggc cattggcgta tctcaaggtt cccggcatgg
     1081 aacacatcga ccggttctat tattcaaacg aaaacgtcga ccagcttacc gtatcccgct
     1141 tttacggtta tacgctcgag gacaccggag cgggcgccct gcgccagctc gccccgtacc
     1201 tcatgtatgg ccacatgctc tcggcggacc gcgccgtcga ttactcggcc atgctgggca
     1261 ggatcaccac gcctaccctc ctcattgccg gagacggcga catcatgtcc gacgtaccgt
     1321 ccaccgagct cacactcgct ggcctggcca gcccggacaa gatgatgatg agatttggca
     1381 cctccaatgg ccacgtggcg gattacggcc actgcgatct ggtctggagc cgctatgccc
     1441 cgagagagat ttttccgccg atcatcgact ggctcgacca gcgccagccg ggcgcgggac
     1501 cgagccccca atccatcagt cccttgcacg acggtacgat ctcagctagt ccgcaaaatt
     1561 gattcgatgc agatttgcga ccaagtttca aattcgtgcc ggaacgtcac gacgcgcgag
     1621 agcgggaaaa accgagcctc ggcaagccct tcttcccgtg ccgtcacgtt tggttgttcc
     1681 agctcgaaga gatggaccac gcccagatgg acccgaccga ctggggtcga atcatcattg
     1741 atcaggccga cccgaccaga ccggcccggg ctggcaaccg ctacctcttc atcgagttca
     1801 cgcctcaggg ccgcctcgta cgcatccagg tccttacctc cttgtgcgtc ttcttcggac
     1861 acgtgcccgc cgacccctag cgatcgcagt cggtgcagcc tggcctcgcc ttgtgatttg
     1921 ccacgcgtgt aacagaagac gcttccatta cagcgaaata ccacgtacgg gatgatctgc
     1981 ttgtagcccg ggtcattttc gacctccgat ctgggacgat attgcatcag ctctgggacg
     2041 agcagggctg tgatgtagcg gtcgacttcg cccgagaaac cctgaaatcg gcccagacgg
     2101 tcaagttctg tgctgggaac gaccagaaca cgttcgtcga ctttttggct catcaccggt
     2161 ctcgcggagt ttggtgtcta cctcgtgaga aacatgatgc gcgattgcct acttcgcctt
     2221 caagactgac gatacaagcc tggaagaggc cgttggccac gcgcatgcgt gccccgcgaa
     2281 accaacgaga cgaaagcggt cgcaccgccg tgacgtacgt catcgattgg aggacacgcg
     2341 ccgctccctg gccggttcac gacggagctt gctttttggt gacctgatcg ggaaccgcaa
     2401 gcgaccgatt aaccaactcc ggcaagtatt tcacgggaat tgttccatgc gacaggacag
     2461 cctcgtgata gcgcgcgaga tcaaactggt caccgagcgc tcgttgaatt tgctgtcgca
     2521 gtcgataaaa ggccgtgcgg cccacgaaat aggtcgatag ctggcaggaa gtctgtttgg
     2581 cgcggatgat cttgccgatc gcctcgccct cggtctggaa cgcccggccg acgagcaggt
     2641 ccatcgcttc cgcatcgctc atcgttcccg catgcatccg gtggtcgaga atcgcgttga
     2701 ccaccgcacg gagataaaac ttcaattgct ggagccgcag gcccaggtct gcctggccaa
     2761 acccttggtc gagcatcatc tgttctgtgt agacggccca gccctcggcg aaagttcccg
     2821 aggaaagcac gcgccggatc aatgacggac accggttgct gtattcaagc tgcacatagt
     2881 ggcccggata ggcttcgtgc acagtgagga tcttgagcat cgaccggttg tattcgcgca
     2941 aatagctttt ggcacgctct ttcgaccaat cgcgcggagg ggggctgatg gcatattcac
     3001 tcgagccccg gatatccaag ggcggcgctg ggtttaaata ggcgaccgag ttgccgcgga
     3061 ggaactcggg catgaggacg atccggcacc ggtcgggctc aatcagcgaa ataatcttgt
     3121 gagttgtgat aaacgtcttg atctcgccga cggtgccttg gacatccgcg accaatgact
     3181 ccgctgtgcc atggtggctg gcgacgtcgt ccagcacctg ctgaatcatt acgcggcgcg
     3241 gttggggatc atcaatcggc aagggcctcc cgggaaaaac cgtgccccac atctgccgtg
     3301 cgacgagcgc catctctgac tccacgcggg ccgcctctct ttcggcttcg gccagaacga
     3361 ggtcggcgga cagacccgcg tcgagctcga gctcgagctt cttggcatac ctggacggac
     3421 cgatgcgcca ttcatcaacg gcatgaggca ggacttcgtt tttgagatat gccacgtgcc
     3481 ggttgagcga ggcgacgatc cgctcggctt tcgagctcag ctcgtcatgg ttcggctttt
     3541 gacctgctag ctggaacagt tcggacgaat aaaatccgat ggcgccctcg gcctgtctga
     3601 tcgccgtctc gaccttgacg cgaactgggt tcttgatcgt cgacctggcg atatcgatcg
     3661 cgcgaggtat ctttgccatc cgagccaacg cgttcttgag gttaacctcc ttgggaaggg
     3721 aagattgagt tagcaagaga tagacgctct cggtgatata tgatccgtac acacgcgggt
     3781 cttcttcgaa cggatggaac gtctcgctca gccaaatgtt gcgttccagg tgctgccgga
     3841 aaatcccgta atcgatctga ccgtcacgcg atagattcgc gaagttgact ttttggccta
     3901 gctcggcgag agccgcgcga tcgtggttga gaatagcgtt gcgcgccggc tgcgacagat
     3961 catcgagttg gtcgtcgaat cgatgctcgc ccagacgcgt cgccatcatg ggctcaaggc
     4021 gaaacgcggc ttcaagatag tcgcgaaaga acgattctaa gacggcgttt ccgttattgg
     4081 ccggttggtc gccacgaacc gtcgaagcga cgtcccataa tgagccgata acgacacaga
     4141 cttggatcaa gcgcatcaat cgaccactcc cacgttggca ccacactgga ttgagttgga
     4201 cgtcgcaccg ctcgttgcca gctggtcaca agtgctcccg tttcaaagcg aagcgccgag
     4261 tagtgtacat tgacagaaag tgcgtgtcgc gaatataata gcctttttgc caaccgcaac
     4321 aaaaacatgg caatttatct gagataccga tttttgagtc tctctgctcc atgaagaaac
     4381 tcgtctccgg gttgttggca ccggtcgtgg caatttgggg cgaggccttt cactccatgc
     4441 agctcttatc ttttcgtgtc aaactattga tttgagtacg gtcaaatcag cctgggtccg
     4501 tcgccacgcc cttttgcgag gcatggcgag caaagaccgc ggcggtttct ttttgtccga
     4561 tccgaagaat gtgccaagtg gttctgatgc ggcccggtgc gacgatttac gacgagcaaa
     4621 atcgcgtaca gggagttctc gacatcccgc tttccgagcg cgggaaaggc gaagtagcac
     4681 ggatggccca gaagctgtcc cgatgcatca atgggtcgcc gttgtcagct ctttactgtg
     4741 ggcccgggga aaatgtgatc ggatccgcgg aaatcgtcgg caaggtcttg ggaattcggc
     4801 ccaagcggat tgacgaattc cgtaaccttg accagggatt gtggcaaggg ctccagatcg
     4861 acgaaattcg ccgtcgcaac accaagttat ttcggcagtg gatcgacgac ccggaaacga
     4921 tctgtccacc gctgggcgag acggtcgaat gcgcgatggg gcgaatcaag gcggcgtttc
     4981 ggccgttgct caagcggcat cacgacgagg cgatcggctt ggtcgtcggc gagcctcttg
     5041 ccaccatggt agcctcttat ttgaaatgcg agccgcgcgt tcatctcgat gaacactggt
     5101 cgtgcggcgg ctttgaatgg attgagatcg ccccgttgct cttgcgcgat ggttcggctt
     5161 cttgaacgaa aagccgcggc gagattaggg ggggccgggc actggcgctt tcgcagccgc
     5221 gaaacgagtg agggctgaca aggaaaaaaa gcgatggcag cgcgggacgg caaagcgaaa
     5281 aacgcttggc atggacattt cgagccgaag cgcgtgcccg aaggcatctg gatgccttgc
     5341 gacggatgcc aggccacgct cgtccgcaag caagtcgaac aaaatgacgg catttgcccc
     5401 gaatgcaatc atcacttttc attgaccgca gccgagcgca tcggccagct cctggatccg
     5461 gatacgtttg aggaatggtt ccctggcctg caaactttgg atccgctcgg attcaacgac
     5521 cgtcgaccct atccagagcg catcgaggcc gagcaggcac gcaccggcct cgctgaggcg
     5581 gctgtcgtcg gccagggatt catcaaggga atccggatcg ttatcggcat caccgacagc
     5641 agtttcatca tggggagcat gggctcggtc gtcggcgaaa aattgactcg agcgatcgaa
     5701 gaggcgacgc ggcagaaact tcccctggtg atcgtttcag gttcgggggg cggggccaga
     5761 atgcacgagg gaatcttctc gctgatgcag atggctaaag tgtcgacagc cctcgggcgc
     5821 tatcacgccg ccggaggaat ctatctgagc atcctcaccc acccgaccat gggaggcgtc
     5881 gcggccagct ttgcctcgct gggagatttt atcctggccg agcccaaggc cttgatcggc
     5941 tttgccggac cgcgtgtgat cgaacagaca gtccgggttc aacttcccac gggatttcag
     6001 accagcgaat tcttgtgcga acacggtttt atcgatcgaa ttgtgaatcg gcgcgacctg
     6061 cgcagcacga tcgcacagct catcgactat gcgatgcctt gaaccaaggg ccggcatgcc
     6121 tctgaccgct cggcacgtca atcgtgcctg agcaattcga gcaagagatt atcctgtggg
     6181 tacgggtgtc ccttgtactg ccggaacgcc tcggcgtagg ggacgccgat ctcggccccg
     6241 cggcgggctc cccattttgc ctggatgtcg agatactcgt cgatgccgtg ctgtcgcatg
     6301 agccagttgc gctggctggc gtgacaggcc agcatctctc gcttggtcgc aaagaccggc
     6361 gaaatatcga cgtgaaaatc aaccggcaca ggccgtccgt cgcggtcgac gccttcgagc
     6421 gggtcgacgt aatagaggtg cggaatcgtt tcgagcgcgg gcgcgggctc ccattgccgc
     6481 gtttgatagt tcgggcacgg ggctgcgaag cacgcgtcac gtaccaggag actggtcatc
     6541 tcatgatccg cgatgtagtc agctggtggg gccgtgagaa tcaatgcggg tgcgacccgt
     6601 cgcacggcct cggtaatgcg gcgtcgcgat tcatcgtcgt taaagatggc gagatcgcgg
     6661 aattcgagac agatataatc ggccccgatc agctcggccg cttttcgcgc ttcctcacga
     6721 cggatggccg agatcgactc ggcatcgtat tcggcactcc cgcagtctcc aggcgtcatg
     6781 gtggcgatcg tcaccgcgac accggcgtgc cgcagcaagg tcaaagtacc agcacactgg
     6841 aattccacat cgtccggatg agcatgcaag gcgagcacgc gtatcggttt gggcatgatt
     6901 gattctcgac gagtccacgg cgaatttgtg ctgtttgaca tgtaatgcgc ctgcctgctg
     6961 tcggcaagca ccttgtcctc gaacacgagt ctgctggtca ggtataccga gaaattcggt
     7021 gttttcttaa aaatgttctc gcgtgcgcta gccaacctgt gtacattagc aatcaaagag
     7081 tcacttactg atcgcccgtg tcatactcag gaaacagctt ggtacgggtc atccaccttt
     7141 ggaccccaat tcagtttggg aggaatatta tggccgacct cgacgcgctg acccctcggc
     7201 aaaaggaaat ctacaatttc attcgcagca agatccaggg ccgcggttat ggcccgacgg
     7261 tccgcgaaat cggcctccat ttccagatca agagtcccaa cggcgtcatg tgccatttga
     7321 aggcgctgca gaaaaaggga ttgattcatc gcgaaccaaa catgtcgcgc gccatccagt
     7381 tgctggatga cccggtgagc gcgcatcaaa tggggcttaa gctcgtgggc cgcatcgcgg
     7441 cgggacaacc gatcgaggcg gtcgagcaac acgaagagct tactttcagc gactggaccg
     7501 agcccggcga taaattcgcc ctgcgcgtcg ccggcgactc gatgatcgag gagcacatcg
     7561 ccgacggcga tttcgtgatc atcaagcggc aagagcaagc gcgcgatggg caaatcgtgg
     7621 ccgtgcgtga cgacgatgga gaggcgaccc tcaagaagtt tttccgagat cgcaatcggg
     7681 tccgtctgga gccggcgaac aagacgatga agccgatctt cagggatcgc gtcaacgtct
     7741 tgggcgttct cgtgggcgtc gttcgaaaat actgacggtc cgcgggcggt agctgctcgt
     7801 ccaccgggtc gtatagaatc tcgattcctc agactctagg atttgactca gctgggaatc
     7861 tggttatcta cggctcgtgc gctgccgcgc cctctgtggg gcatgggaag gacgattaga
     7921 tgggaaagga ctcctgcgag aacggcgaag tggtccgatt gttcacggac ggcgcttgta
     7981 gcggcaaccc agggccgggg gggtgggcct tcatcctcca gcaccctgcc agcggcaaaa
     8041 gcagggattc ctcgggtgcc gagccggtga cgacgaacaa ccgcatggag ttgatgggtg
     8101 tcatcgaagg gctcgcgagc cttaaacgac catgcgaggt cgaactggtc accgatagcc
     8161 aatatgtcgc caaagggatc agcgagtggt tgccgaactg gaagcggcgg ggttggcagc
     8221 ggaaagagaa gggccaattg aagccagtcg cgaacgtcga cctttggcaa aaggtcgact
     8281 cgctaatgag cgtccacaag gtttgggtga ctcacgtgct cggacatcgc ggacatcccg
     8341 agaacgaggc atgcgaccgc atggctgtgg acgcctacaa gaagctcgtg gaagaacaaa
     8401 aagtccagag tcaatccaga ctttgacgac gatttccacc acagcggcat ctccaggcta
     8461 tcggaattgg cacacaccgg cgagtgtgcc agcgac
//
LOCUS       DATAQL010000586         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3724023, whole genome shotgun sequence.
ACCESSION   DATAQL010000586 DATAQL010000000
VERSION     DATAQL010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724023"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VHS97_10235"
     CDS             complement(<1..773)
                     /locus_tag="VHS97_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448621.1"
                     /translation="MTWNGRLNRWFHGGLIGLTFALAIVAATDCRADSVIMKNGIVYR
                     GQGAPDRDNTLVFISDGLKRVVVRDSKIERIEPNNAFRTGEKFQLVQPMTVHGGIMPD
                     EVVSVQASPWNERGRRSFQYVGARLDKTIRMEQAIIEIGPHITRFRGVDGFWVGMIET
                     NQVPRESVTKLLGRVQQNNAEERERVVRFLMDVGWLAEAKQELDRLVKDFPDPNLKER
                     AASARQFIIQAEALERRREIDVSKRAQQYDRAAKLLKSF"
     gene            1055..1786
                     /locus_tag="VHS97_10240"
     CDS             1055..1786
                     /locus_tag="VHS97_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448622.1"
                     /translation="MAADRLKFRCYRCNQLLAAAPGKAGAVVTCPKCKAELQVPAPEP
                     SVFAEVAPRQQADTSGRAATTEAPPKPPPIPAYIQEIAAAIPAEVADLRPEDLRVEAE
                     FFESLTRTPARPPDPEPVPSFGPDLNASFPTLDPQAAPVGGLALPEIDVNRPAQRDFT
                     VEPSTLQGISRPADTPTTDIHPDAPPIEIEPPTILPPGTEMRPIHEVVLPASVVLTWS
                     LFGLIGIATSFIAGLFIGHYFWVAH"
BASE COUNT          500 a          822 c          758 g          509 t
ORIGIN      
        1 ttaaatgact tgagcagttt cgccgcgcga tcatactgct gtgccctctt gctcacgtcg
       61 atttcgcggc gacgctcgag cgcctcggct tgaatgatga attgacgcgc actggcggct
      121 cgctctttca agttggggtc gggaaagtct ttcaccaggc ggtcgagttc ttgtttggcc
      181 tcggcaagcc agccgacgtc cattaaaaac cggaccacgc gttcgcgctc ctcggcatta
      241 ttctgctgga cccggcccag gagtttcgtc accgactcgc gcggcacctg gtttgtctca
      301 atcataccga cccagaatcc atcgacgccc cgaaagcggg tgatgtgcgg gccgatctcg
      361 atgatcgcct gctccatccg gatggtcttg tcgagcctgg cgccgacgta ctgaaacgag
      421 cggcggccgc gctcattcca cggactcgcc tgcacgctga cgacctcgtc gggcatgatg
      481 ccgccgtgca ctgtcatcgg ctgaaccaat tgaaacttct cgccggtgcg gaaagcgttg
      541 ttgggctcga tccgctcgat cttggagtcg cgcactacga cccgcttcag cccatcggaa
      601 atgaacacca gcgtgttgtc tctgtcggga gccccttggc cgcgatagac gatcccgttt
      661 ttcatgatca ccgaatcggc ccggcagtcg gtggcagcga cgatggccaa tgcgaaggtc
      721 aagccgatca agccgccgtg aaaccatcgg ttcaaccggc cattccacgt catgctgggt
      781 ttcctcgtga ccgtaccgaa ctccatcaac gatgacgagc acgggcggga tccgcactcg
      841 gcccatttgc tcattgtgac acccctgccg aacaggtcaa gccgccacgt tgttggacgt
      901 cgccggccga gtagctattc gggggcggcg gctatctggc tcatcggggt tggtagggtg
      961 gcgaacgacg ccgaggccgc gtaatctaga ctcaaaggat tttgcggaat cgactgacat
     1021 ccacgatcga tgacacgggg aaaggtagtt tcatttggcg gccgatcgtc ttaaattccg
     1081 ctgttatcgc tgcaatcagc tgctggcggc cgcgccgggc aaggctggcg cggtggtgac
     1141 atgtcccaag tgcaaggcag agctacaagt tccggcccca gagcccagcg ttttcgccga
     1201 ggtggcaccg cgccagcagg ccgacacttc cggccgcgcg gcgaccaccg aagcgccgcc
     1261 caaaccgccc ccgataccgg cctacatcca ggagatcgcc gcggcaattc cggccgaagt
     1321 ggccgacctc cgccccgaag acctgcgagt tgaagccgag tttttcgaga gcctgacgcg
     1381 cacgcctgca cggccgcccg atccggagcc cgttccctcc ttcggtccag atctcaacgc
     1441 ctcgtttccg actctcgatc cgcaggccgc cccggtcggt ggactggctc ttccggaaat
     1501 tgacgtcaac agaccggcgc aaagggattt caccgtcgaa ccgagcactt tgcaggggat
     1561 ttcccgccct gccgacacgc cgacgacaga tatccatccc gatgctccgc cgatcgagat
     1621 cgaacccccc acaatactgc cgccgggcac cgagatgcgg ccgattcacg aggtggtctt
     1681 gcccgcctcg gtggtgctta cctggtcgct ttttggcttg atcggcatcg ccacatcgtt
     1741 catcgcgggg ctgttcatcg gccactactt ctgggttgcg cattgacgtc tccgagttga
     1801 cagtcgtcgt cagttcgaac aaatgagagc gtcgtgaaga atgctgacac ggcccggaaa
     1861 aagaaaaacg gacggcagtg gctcgcttcg cccggggccc agactggccg gcacgccctc
     1921 gaggggcaag taatgaaatg taacggttga aacggacgta gcggttttgc ggctacaaaa
     1981 tgccagcagg tggcagtttt cgggaacggc tccgagccgc ctcgccccgc gcgatgcgat
     2041 cgacaacgat ttatcgcaat cgttcagcgg aattccgcaa ccgccaatac gcgaacaatg
     2101 aggaaaccag gaaaccagga actgtccaaa cctgccttct cctgccttcc tgcgttcctc
     2161 attcggtccc ttcctcattc agctgaacgg ttacgattta tctagacggc atttcgtgat
     2221 tccgggaggg ccaggctcat gccgagccgg atggcgccgt ggctcgccag gatgtgggct
     2281 tgatggcggc agaaaaatat tctcaaaaat aattcggggc gacgaattgc gcgcctgtag
     2341 gcgcgccggc gcgaaaatcg gcgaccttgc ctggccgagt ggcgcaagca ccgggcgggg
     2401 ccagagcggt gccctggcat ggccaccatc gcaaagttcg taagcaccat acgaccgatt
     2461 taactctggt cgcccccgaa tgggagcgga agcctgctac gacctacggc cgagttgtcc
     2521 acgggacggt ctagggaggg cctccgattc tctccatact acccctggcg catcttcttc
     2581 gacagctcg
//
LOCUS       DATAQL010000587         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4333168, whole genome shotgun sequence.
ACCESSION   DATAQL010000587 DATAQL010000000
VERSION     DATAQL010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4333168"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1725)
                     /gene="purD"
                     /locus_tag="VHS97_10245"
     CDS             complement(217..1725)
                     /gene="purD"
                     /locus_tag="VHS97_10245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX3448623.1"
                     /translation="MKVLVIGRGGREHALCWKLKQSPRVTSVICAPGNAGTAIDCQNV
                     SIEPGDTRGLVQFARREGVGLTVVGSEEPLVKGIVDVFQREGLRIFGPRKEAAELEGS
                     KLFAKDLMRQAGIPTADYRVFRSGPDAEHYAMSREVALIVRSQGRSTIRHTLHCRTAK
                     ETLDAIDRIMDPREMLSPGVRVEIEERGQKRVFSTAAEARDFVLGRPLGMVLKADGLA
                     AGKGVYVCDNLRQALAGIDQIMVRRIFGQAGDRLLIEERLDGREASVLAITDGRTIIP
                     LESSQDYKRAFDHDEGPNTGGMGAFSPTAGLDRELMEQVESEILVPVIHALKRARRPF
                     RGVIYAGLMLTNQGPKVLEFNVRFGDPETQAVLMRLKSDLFEALQAAVDERLDTVNLE
                     WDPRPAVTVVMASEGYPGNYERDRLIQNLEVVDRIPDVKVFHAGTALRADPALGREGR
                     IVTDGGRVLNVTALGATVEEARARAYEAVRTIKFSGAWYRRDIASPPARTVH"
     gene            2107..>2243
                     /locus_tag="VHS97_10250"
     CDS             2107..>2243
                     /locus_tag="VHS97_10250"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="catalyzes the formation of 4-aspartyl phosphate
                     from aspartate 4-semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX3448624.1"
                     /translation="MRAKHVAVVGASGAVGERMTQLLEERRFPLASIKFLASARSAGK
                     S"
BASE COUNT          407 a          726 c          695 g          415 t
ORIGIN      
        1 ccctacgctc cgtacccggc tccgacggtc tatcacaagg ggcaaaacgc cttgaggggg
       61 ctgttcaaga tcgaccacca ccccaggatc atcaagaaat agcggcgtcc ggacgggccg
      121 ccaggcagag aatcgtggtc acgagacggc tcggggaaac gcaagaagtc ggcggctccg
      181 gctgatttcc gccaggttgg cctatatgac cccagattaa tggaccgtcc gagcgggagg
      241 actggcgatg tcgcggcgat accaggcgcc ggagaacttg atggtccgta cggcttcgta
      301 ggctcgagcg cgggcctctt ccacggtggc gcccagtgcg gtgacgttga ggacgcgtcc
      361 gccgtcagtg acgatccggc cctccctccc cagggcgggg tcggctcgca gcgcggtccc
      421 cgcatgaaag accttgacgt cggggatgcg gtcgacaacc tcgagattct gaatcaagcg
      481 gtcccgctcg taattgccgg ggtatccctc ggaggccatg accacggtca ccgccggccg
      541 cgggtcccat tcgaggttga ctgtgtccag ccgttcgtca accgcggctt gcaaggcttc
      601 gaacaggtcg gatttcaacc gcatgagcac ggcctgcgtt tccggatcgc cgaaccggac
      661 gttgaattcc aggaccttgg gcccttgatt ggtgagcatg agcccggcat aaatgacgcc
      721 gcgaaacggc cgcctggctc gtttgagcgc gtggatcacc ggaacgagga tctcactctc
      781 gacctgttcc atcaactcgc ggtcgaggcc cgcggtcggc gagaacgcac ccatcccgcc
      841 ggtgttgggc ccttcgtcat ggtcaaaggc gcgcttgtaa tcctgacttg attccaacgg
      901 aatgatcgtc cggccgtcgg ttattgccag cacgcttgcc tcgcgtccgt ccaggcgttc
      961 ctcaatcagc aagcggtcac ccgcctgccc aaagatcctc cggaccatga tctggtcgat
     1021 ccccgccaac gcctggcgca ggttgtcgca gacgtaaacg cccttgccgg cggccagacc
     1081 atcggctttc aagaccatgc ccagcggccg ccccaatacg aagtcgcgag cctcggcggc
     1141 cgtactgaag actcgctttt gcccgcgctc ttcgatttcg actcgcacgc ccgggctgag
     1201 catctcgcgg gggtccatga tccggtcgat ggcgtccagg gtctctttgg ctgtccggca
     1261 gtgcaacgtg tggcggatcg tcgagcggcc ttgcgagcgg acgatcaggg caacttcgcg
     1321 cgacatcgcg taatgctcgg cgtcgggccc cgagcgaaag acgcgatagt cggcggtcgg
     1381 aatgcccgcc tgccgcatca ggtccttggc aaagagcttg ctcccctcga gctcggcggc
     1441 ttccttgcgc ggaccaaaga tccgcagacc ctcgcgctga aatacgtcga cgatcccttt
     1501 gaccagcggc tcctcggagc ccacgaccgt caggccaact ccctcgcgcc tggcaaactg
     1561 caccaggccg cgcgtgtcgc ccggctcgat cgacacgttc tggcaatcga ttgccgtccc
     1621 ggcgttgcca ggagcgcaaa ttaccgacgt gactcgcggc gattgcttta gcttccagca
     1681 gagagcgtgc tcccgccctc ctcggccgat caccaagact ttcaccgcgc tacccccctc
     1741 gtcctcaagt tgatccgtgg ctccgaccgt gctcccattc tatagacgac gaagggcgac
     1801 gcacagttga ctattgtccg ctcgacgcgc agtagatcct ggcgcgcggt aatcggctga
     1861 tcggcgtgtt tcgcatcgac ctggtcgacc tgtctggatc gcctggattt tgcggcggcg
     1921 agcgcgtatc gtaggaagtc aaagccggga aacaaccttc agttcgccgt cacgcaattt
     1981 ccgacccgag cagaacgaca cagcagaccg ttatgcgcga tggcagcagt tggggtgaac
     2041 cttttgggcc tggcgacaat ccatgagtga ggtcgatcgg ctcgagcccg cgaggacaga
     2101 gagacgatga gagcgaagca tgtcgccgta gttggggcga gtggggcggt gggtgagcgg
     2161 atgacccagc tcctggaaga gcgacgtttt ccgctggcat ccatcaaatt cctcgcttcg
     2221 gcgcggagcg caggcaagtc gat
//
LOCUS       DATAQL010000588         5092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4487275, whole genome shotgun sequence.
ACCESSION   DATAQL010000588 DATAQL010000000
VERSION     DATAQL010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4487275"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..512)
                     /locus_tag="VHS97_10255"
     CDS             complement(201..512)
                     /locus_tag="VHS97_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX3448625.1"
                     /translation="MKQHISSIQTNVTVFVVLLVLLFATLGAAYLPLGRLHFPTAMAI
                     AAAKAVLIVVYFMHVKYSHRLTAVIAVASLLWLGIMIALTLTDYLSRSSPGSWLNIPG
                     K"
     gene            complement(509..1228)
                     /locus_tag="VHS97_10260"
     CDS             complement(509..1228)
                     /locus_tag="VHS97_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEX3448626.1"
                     /translation="MNTATTHHIEHGSTQVDDEPILSHQFDDVDQQTEASTLGMWVFL
                     ATEVMFFGGLLTAYAVYRATAPREVALASEELNVLLGCINTVVLLSSSLTMAMAVRAA
                     GLRAPRELVMWLVMTMALGTAFLGIKAVEWTADYHEHLIPGLNFQVPEKDRALVEREG
                     LNPGKMELFFVLYFFMTGLHAIHLIIGIVLVGIMAYLSWRGWFSGGGSTQIEVTGLYW
                     HFIDIVWVFLYPLLYLIDVHK"
     gene            complement(1200..2846)
                     /gene="ctaD"
                     /locus_tag="VHS97_10265"
     CDS             complement(1200..2846)
                     /gene="ctaD"
                     /locus_tag="VHS97_10265"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX3448627.1"
                     /translation="MSVTTAVEFKEDYLHAYTLKSWLLTTDHKRIGLLYMASITLFFF
                     VGGAAATLMRLELMTPKGDVLHSPDAYNRLFTMHGVVMIFFFLVPSIPAVMGNFLIPL
                     MIGARDLAFPWLNLLSWYVYMIGGSIALWILIFGGVDTGWTFYTPFSTMFSNTMVVPA
                     ATAVFITGFSSILTGLNFIVTIHTMRAPGMTWFRLPLFVWSHYATSLIMVLGTPVLAI
                     AVGLVAVERLFRVGIFDPALGGDPILFQHLFWFYSHPAVYIMVLPGMGVVSEIIPCFS
                     RRPIFGYRFIAYSSMGIAVLGFLVWGHHMFVSGQSMYAGMVFSILSFLVAVPSAIKVF
                     NWTATMYKGSIRLDTPMLYALGFIGLFTLGGLTGLMLASLGLDVHVHDTYFVVAHFHY
                     IMVGGAVMAYMGAIHFWWPKVSGRMYSELLGKLSALTVFVGFNLTFFPQFVLGYLGMP
                     RRYYEYAPEYQVLNVMSTAGASILAVGYVLPLAYLLFTLTRPPTAGPNPWRATGLEWQ
                     TDSPPPVHNFDEPPVVTRAPYAYYPAGTEGFDEYGDHSSH"
     gene            complement(2843..3793)
                     /gene="coxB"
                     /locus_tag="VHS97_10270"
     CDS             complement(2843..3793)
                     /gene="coxB"
                     /locus_tag="VHS97_10270"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX3448628.1"
                     /translation="MWNFPLFPDQASTMAKQVDSLYIFELGIAGFFTALICVFILAFA
                     ARYRQKANVDRSNPPIASKLLEVIWIGVPFVLSMVMFAWGAVLFFRIYEPPGDALEVA
                     VVGKQWMWHLQHAEGRAEINELHIPLGRPVKLTMTSQDVIHSFYVPAFRVKQDVLPGR
                     YTTLWFEPTKVGRYHLYCAEYCGTNHSTMGGWVSVMEPVDFQRWLSQGGAGPSMADEG
                     ERLFVQHHCAGCHRGSQTVKAPVLEGVFGQPVPIQAGNEVRFVTADATYIRDSILMPK
                     SQVVAGYEPLMPSYKDQISEPDLLKIIAYIKSIGAEGASQ"
     gene            complement(3809..4693)
                     /locus_tag="VHS97_10275"
     CDS             complement(3809..4693)
                     /locus_tag="VHS97_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3448629.1"
                     /translation="MTHKTRILVVAILLGLGCLLSSSAVRAQQTVGGVVSQVGFDQKL
                     GIQLPLDLRFHDDRGRDLRLGELFGRRPLILAPVYFRCPLLCNQLLNGLTRSLKPVSL
                     AAGKDFDVIAFSINPAETPELAGPKKQAYLEQYDRPGSDNGWHFLVGDQSAITALTEA
                     IGFRYTFNPQTELFAHAAGVVIVTPEGKIARYFYGIDFPPKELENELARARAGRIGNP
                     IGRLLLLCYDYDAATGKYTLSIMRLLRIAGTATAAALGAFVCLMFRREAKERRKKNTG
                     HEPGLPMDERDHGSLPLC"
     gene            complement(4690..>5092)
                     /locus_tag="VHS97_10280"
     CDS             complement(4690..>5092)
                     /locus_tag="VHS97_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448630.1"
                     /translation="ALFGIGILVEYGLGFVMRDFSREEKGLEALAPPTFPDDSGPFPG
                     PRLQAEPSTDFQKMRKGALSHLNGYGWVDRKAGIAHIPIDRAMEIVVRSGLPTAVGSG
                     ASGAAASPEAKPLPPEAGKPASPEAKQDKKP"
BASE COUNT         1163 a         1568 c         1466 g          895 t
ORIGIN      
        1 cactgtggct gatcgctcgc tgaccacccc actccgttgg atctcactgt ggctgatcgc
       61 tcgctgacca cccgcctcct ttgggtctca ctgtggctga tcgctcgctg accacccccc
      121 tgccccccct tcacaagggg gggaaaggaa ctcgcgctcc gaggtggtgc gccgcttcgt
      181 acaacgggcg tctgacgctc tcacttgccg gggatgttga gccagctgcc gggcgaggaa
      241 cgcgacagat agtcggtcaa cgtcagcgcg atcatgattc ccagccagag tagcgatgcc
      301 accgcaatca cggcggtgag gcggtggctg tacttgacgt gcatgaagta aacgacgatc
      361 agcaccgcct tggccgcggc gattgccatt gccgtgggga aatgcaaccg ccccagcggc
      421 aaataggccg cacccagcgt cgcgaacaac aggaccaaga gcacaacaaa cacagtgacg
      481 ttcgtctgga tcgaggaaat gtgctgcttc atttgtggac gtcgatgaga taaagcaagg
      541 gatagagaaa cacccagacg atgtcgatga agtgccagta caaaccggtg acttcgatct
      601 gagtcgaacc accgccagaa aaccaaccgc gccacgacag ataggccatt atgccgacca
      661 atacgatgcc gatgatcaag tggatggcat gcagcccggt catgaagaag tacagaacaa
      721 agaacagctc catcttaccc ggattcagac cttcccgctc cacaagcgct cggtccttct
      781 cgggcacctg aaagttcaaa ccggggatca agtgctcgtg gtagtcggcg gtccattcga
      841 cggccttgat accgaggaag gccgtgccca gagccatggt cattaccaac cacatgacca
      901 gctctcgcgg cgcccgtaac ccagccgcgc gcacggccat ggccatcgtc aggctgctgc
      961 tcaagagaac gaccgtattg atgcaaccga gcaacacatt gagctcttca ctggcgagag
     1021 caacctccct cggggccgtc gcacggtaaa cggcgtaggc cgtcagcaag ccgccgaaga
     1081 acatcacctc ggtggccaga aacacccaca tacccaacgt cgaggcctcg gtttgctgat
     1141 ccacgtcgtc gaattgatgc gacaggatcg gctcgtcgtc gacttgggtc gacccgtgct
     1201 caatgtgatg agtggtcgcc gtattcatcg aaaccctcgg tgcctgcggg atagtaagcg
     1261 tagggggccc tggtcacgac gggtggctcg tcgaagttgt gtactggcgg aggagaatcg
     1321 gtctgccact cgagtccagt cgctcgccag gggttcgggc ccgcggtcgg gggccgggtc
     1381 agcgtaaaca agaggtaagc caggggcaac acgtagccga ccgcgagaat cgacgccccg
     1441 gccgtcgaca tcacgttcaa tacctgatac tcgggcgcgt actcgtaata ccgccggggc
     1501 attcccagat agcccagcac aaactgggga aagaacgtca gattgaagcc gacaaaaacc
     1561 gtcaaggccg agagcttgcc caggagctcc gaatacatcc gcccgctcac cttgggccac
     1621 cagaaatgga tcgcccccat gtaggccatg acggccccgc cgaccatgat gtaatggaag
     1681 tgggcgacga caaagtatgt atcatgaacg tgcacatcaa gcccgagcga cgccagcatc
     1741 aggcccgtca gaccgccgag cgtgaagagg ccgatgaacc cgagcgcgta gagcatcggc
     1801 gtgtcgaggc ggatcgaccc cttgtacatc gtggccgtcc agttgaacac cttgatcgcc
     1861 gacgggaccg ctaccagaaa gctcagaatt gaaaatacca tacccgcgta catcgactgg
     1921 cccgagacga acatgtggtg gccccagacc aggaacccca gcaccgcgat gcccatgctc
     1981 gaatacgcga taaagcgata accgaaaatg ggccggcgcg agaaacaggg aatgatctcg
     2041 ctcacgaccc ccatgcccgg aagcaccatg atgtagaccg cggggtgcga ataaaaccag
     2101 aacaagtgct gaaacaagat tgggtcgccc ccgagcgcag gatcgaagat gcccactcga
     2161 aacagccgtt cgactgccac aagccccacg gcgatcgcca gcaccggagt tcccagcacc
     2221 atgatcaggc tggtcgcgta atgcgaccac acaaacagcg gcaaccgaaa ccacgtcata
     2281 ccgggcgccc gcatcgtgtg gatcgtcacg atgaagttca agcccgtcag aattgacgag
     2341 aagcccgtga taaatacggc cgtcgccgcg ggcaccacca tggtgttgga gaacatcgtg
     2401 ctgaacggtg tataaaacgt ccagccggta tcgaccccgc cgaagatcaa gatccaaagc
     2461 gcgatcgacc cgccgatcat atagacgtac cacgagagca ggttgagcca agggaatgcc
     2521 aggtcgcgcg ccccgatcat gagcggaatc aggaaattgc ccatcacggc cgggatcgac
     2581 ggcaccagga aaaagaagat catcaccacg ccgtgcatcg taaagaggcg attgtacgcg
     2641 tcgggtgagt gcaggacgtc ccctttgggg gtcatcagct cgagccgcat cagagtagcg
     2701 gccgctccac cgacgaagaa aaacaaagtg atcgaggcca tgtacaagag gccgattcgc
     2761 ttgtgatcgg tcgtcagcag ccatgacttc agcgtgtacg cgtgaagata gtcttccttg
     2821 aattcaaccg ccgtcgttac gctcattgac tcgccccctc cgctccgatc gacttgatgt
     2881 aggcgatgat tttgagcagg tcgggctcgc tgatctggtc tttgtacgat ggcataagtg
     2941 gctcgtagcc cgcgaccacc tgcgacttgg gcatcaatat cgagtcgcga atgtaggtgg
     3001 cgtcggccgt cacgaatcgg acctcgttcc ctgcctgaat ggggactggc tgaccgaaga
     3061 ctccctcaag cacgggcgcc ttcacagtct gactgccgcg atggcagccg gcgcaatgat
     3121 gctggacgaa aaggcgctct ccttcatcgg ccatcgacgg tcccgctccc ccctgcgaca
     3181 gccagcgctg aaagtcaacc ggctccatga ccgacaccca cccccccatc gtcgaatgat
     3241 tcgttccaca atactcggca caatacaggt ggtagcgacc caccttggtc ggctcgaacc
     3301 agagtgttgt ataccggccc ggcaatacgt cttgcttgac ccgaaacgcc ggaacataaa
     3361 agctgtggat cacatcctgc gaagtcatcg tcagcttgac cggacggccc agcggaatgt
     3421 gcagttcatt gatctcggca cggccttcgg cgtgctgcag gtgccacatc cactgcttac
     3481 cgaccaccgc aacctcgagc gcatcgccgg gcggttcgta gatccggaaa aaaagtaccg
     3541 ctccccaggc aaacatgacc atgctcaaaa cgaaaggcac tccgatccag atcacttcca
     3601 gcagcttgct ggcaatcgga ggattcgagc gatcgacgtt cgctttctgg cgatagcgcg
     3661 cggcgaaggc caagatgaac acgcagatga gcgcggtgaa aaagccggcg attcccagct
     3721 cgaaaatgta aagactatcg acctgcttgg ccatggtcga tgcctgatcg gggaacagcg
     3781 ggaaattcca catgatggat tcttaacctc agcagagcgg cagggacccg tgatcacgct
     3841 cgtccatcgg caaaccaggc tcatgccctg tattcttctt gcgccgctct tttgcttcgc
     3901 gacggaacat gaggcataca aacgccccca gtgcagctgc ggtcgcagtt cccgcgatgc
     3961 gtaagagtcg catgatcgag agcgtgtact tgcccgtggc ggcgtcgtag tcgtagcaaa
     4021 ggagcagcaa ccggccgatc gggttgccga tgcggccggc gcgggcgcgc gccagctcat
     4081 tctcgagctc cttgggaggg aaatcgatcc cgtaaaaata gcgggcaatc ttcccctcgg
     4141 gcgtgacgat gaccacgccc gccgcgtggg caaacagctc ggtctggggg ttgaacgtat
     4201 agcggaagcc gattgcctcg gtgagcgccg tgatcgccga ttggtcacca acgagaaagt
     4261 gccagccgtt gtccgagccc ggccggtcgt attgctccag atacgcctgt ttcttcgggc
     4321 cggccagctc cggcgtttcc gcggggttaa tgctgaaggc gatcacgtca aaatctttac
     4381 ccgcggcgag cgacaccggc ttcaagctac gggtcaagcc gttcaaaagc tgattacaga
     4441 gcaaaggaca gcgaaagtag accggcgcca gtatcaacgg gcgcctgcca aacagctcgc
     4501 ccagccgcag gtcgcgtccc ctgtcgtcat gaaaccggag gtcgagcggg agctggattc
     4561 ccagcttttg gtcgaatccg acctggctca ctacgccccc cactgtctgt tgcgcgcgga
     4621 ctgcggacga cgacaagaga catcccaggc caagtagtat cgctacaacc agaatccgcg
     4681 tcttatgagt catggcttct tgtcctgctt ggcttcagga ctcgccggtt ttccggcttc
     4741 aggcggcagc ggcttggcct ctggactcgc tgccgcgccc gatgcgcccg atccaaccgc
     4801 ggtcggaaga cccgaacgga cgacgatctc catagcccgg tcgatgggta tgtgtgcgat
     4861 cccagccttg cggtcgaccc acccgtatcc gttcaagtga ctcagtgcgc cttttctcat
     4921 cttctggaag tcagtggacg gttcggcctg gagtcgcggg cctggaaatg gacctgagtc
     4981 gtccggaaac gtcggtggcg cgagcgcttc cagccctttc tcttcgcggg aaaaatcccg
     5041 catgacaaac ccgagcccgt attcgaccag gattccgata ccgaacaagg ca
//
LOCUS       DATAQL010000589         7059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2808333, whole genome shotgun sequence.
ACCESSION   DATAQL010000589 DATAQL010000000
VERSION     DATAQL010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808333"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1156)
                     /locus_tag="VHS97_10285"
     CDS             complement(53..1156)
                     /locus_tag="VHS97_10285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3448631.1"
                     /translation="MSEHPAFHDSQHARLTVIVVNHESWTDAVRLTASLIAEPEFSSG
                     QCRIVVVDNASRGPIPSALLNPPSGLRLVVRSQNEGFAAGVNTGWRLARSPWLLVLNP
                     DVDVASGFLGQVFARIDTHEANPDGPPGVIGFGLHNSDGTPQGSVGVFPTLARTIREQ
                     FIPRSRRKYQAGWRIRSGPVDWVTGACMLVNSELIAALGGMDEDFFLYHEEVAFSRAA
                     RRLHWSVVYDASVTVVHRHPLQNRAISPKMRVITRHSKLLYFLKHLPRWQFLTLVEIV
                     KLEAASQEVWSRLLLRPEEVRAWRVIGDMARRLRSGEVPRGREVLALAELVEKPAPEN
                     RPGPTRPRGAALAETQTGCIPLGSARKHRGARR"
     gene            1402..2532
                     /locus_tag="VHS97_10290"
     CDS             1402..2532
                     /locus_tag="VHS97_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX3448632.1"
                     /translation="MNSPVDILRYLALPSVAAAPSSRVLLVGARRDAERLIHRLANGP
                     WNGPRIVGFVAAGHGRFSSLRPHNRHIALHPQTDPVPVLGSIDRLSELVDRARATHVV
                     VAVSGTPGYHMPPPVTQVINSDVIVQWILVDSGRVDLGSLTSSPPTKTWSVHAVGLPG
                     IRSGIPIWARLNWARSAKRTMDFLIALLALLALAPLFTVVAVSILVTSGRPIFYTQDR
                     VGQGGRLFRIVKFRSMKCDAESETGPIWASDHDKRCTRIGDWLRHSNIDELPQLWNVL
                     TGDMSLVGPRPERPSFVHEFRQTIPKYDLRHAVPGGMTGWAQVHGWRGRTSLRKRIQY
                     DLDYIERWSLGLDMRILLMTVQHVLWGKTSWNESKRPAKTMG"
     gene            2493..3599
                     /locus_tag="VHS97_10295"
     CDS             2493..3599
                     /locus_tag="VHS97_10295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3448633.1"
                     /translation="MERIEAAGKNHGVTTVIGPIDRAIARPVCSVVIPSYNGRAFLET
                     CLASVARQRPRNPALPIEVIVVDDGSTDETAEWLARVHPDVRVARFERNRGFCAAANA
                     GIARANGSFIQLLNNDTEVAAGWIEAGLEPFVDPTVGSVAPLVLVRSEPERVDSAGDS
                     YSLFGWPAKRGHGQPTQLFSERPMEEVFGASGSSAFYRTTALKRSGGFDPLFGSYYED
                     VDLAFRLRWAGYRCVFAPRSIVYHDVSSTYDHGSHALHRRMSRNAEFVFWANLPAVLL
                     GLAILPHLVFLVVQGAWRLLRGRLRPFLAGKWDALRAWQDIKARRRHRAELCRKAVAT
                     PRFALGKGSWRDVRNHLSRPREQTRGHAASLRAR"
     gene            3685..5994
                     /locus_tag="VHS97_10300"
     CDS             3685..5994
                     /locus_tag="VHS97_10300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3448634.1"
                     /translation="MWARILEVLFGILNAGKANAVPRLLGGYWLSSELGPGRIGPTYL
                     AIQLVQGRKVALKSMQPRWAGDATFVARFTREAYAALQITDHNLAQIHDFGDERGIPY
                     FTSEIVDGQSLNALIRAKGRLDIEKAVVYVLQAARGLRCAHQQGILHRDIKPQNLLLN
                     RQGLIKLSDVGLSKTPESAEADDTVGTPTALRIDTGADICSLGCTLYALLTGMQQVES
                     ATVADIKGGQRTQPINPPHPVGEVIPQALSAIVRKMVAAQPEDRYASASDVIAALETF
                     LGISSSLPFNPLDEHASLLDQSVRAYHGSQTALLRSRVKSLFFGACLALAILCLISAR
                     PLAGVGFASLGFLTALADFVLAGIRRQSALFVKLRELIATERMSEWLTALAALAILIV
                     LLMILKLFWLLLGLGFLAIGIVAALHAAFDRRIDADRAKSLAEVERMLRSLRLNGVDE
                     DALRRFVWIHGGTHAEEFYEALFGYDEKREARRRFNRSERSGPRSKFASWRDPIADWL
                     DATVAARRAAGEIVKLQRIEEKSLESLGENLVTARRKGQRSAEAMVALAAEIRKTNRA
                     GDGEYAVNHSIAEAMREAALKPEKVLVQRERGLLPERRRSVGLATIVNMIVGPNVRFL
                     FGAALLAGCIAWMHQNAMISPEHAGALVEAAKSGDVDAIRSHAQAQVAHAREAAAKRT
                     DPLALPVVPAALLNLVSSFGAGVSGLFLIASSVFGGPRIALFAIPAAAVPLLGPQLGL
                     PSMGGLEPSVVPIIIGLALMIAGVVFGRE"
     gene            complement(6040..>7059)
                     /locus_tag="VHS97_10305"
     CDS             complement(6040..>7059)
                     /locus_tag="VHS97_10305"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX3448635.1"
                     /translation="TLNDLIPDLFCMFHLMTTIHFYRLFHMGHHQFTNDPARDPDLQN
                     LGHGKRADEFPMPRHRFITVIYFCFLVDPVRFLRYQWAYFTVNTLGKGKSVYIERAAG
                     AGSATESLPRLATVLGFAYIVGFGLALKLLTATGRPHWLAPAGLAGIAILAGVIRALP
                     DWAVFQSPFRQAYSPRIAGFLRMAYYTFVLVGLAYLRWCTGGRSAIYPVLLWLVPIGS
                     SFMFYMFLRDVYQHSNADSGRLTNSRVFFTDPFTRWAVLVYGQDMHIPHHLFPAVPHY
                     RLRRLHELLKWFHADYRDQVVETHGTFYDNYGRRTILDEMTLPRTDLTVAEPRAARTL
                     GLAGE"
BASE COUNT         1312 a         2156 c         2208 g         1383 t
ORIGIN      
        1 tggaatttgg tcacgcggat ccgtcctttc ggggttcgat aagcgtcggg ccttaacgcc
       61 gcgctcctcg gtgcttcctt gccgaaccga gcggaataca gcctgtttgc gtttccgcga
      121 gggcggctcc tcttggccgc gtgggcccag gacgattctc cggcgctggt ttctcgacca
      181 gttcggccag agccaacacc tcacgtcctc gcggcacctc gccactccgc agccggcgcg
      241 ccatgtcgcc gatcactcgc cacgctctca cttcttccgg tcgtaataat agtcgcgacc
      301 aaacttcctg gctcgctgcc tcgagcttga cgatctcaac cagggtgaga aactgccagc
      361 gcggcaagtg cttcaagaaa tagagcagct tgctgtgccg ggtgatgacc cgcatcttgg
      421 gcgaaatggc tcgattttgc aagggatgtc ggtgaaccac ggtcacgctc gcatcatata
      481 caacggacca atgcaaccgc cgcgcggctc ggctgaaggc gacttcctcg tggtaaagga
      541 agaaatcttc gtccattccc ccaagcgcgg cgatcagctc cgagttgaca agcatgcaag
      601 cgccggtaac ccaatcgacc ggtcccgaac gaattcgcca accggcctgg tatttccgcc
      661 gggagcgcgg tatgaactgc tcccgaatgg tccgggcgag ggtcggaaag actccgacag
      721 agccctgggg agtgccgtcg gaattgtgta agccgaagcc gatgacgccg ggcgggccat
      781 cgggattggc ttcgtgggta tcaatgcgcg cgaagacctg gcccaggaaa ccactggcga
      841 cgtccacatc gggattgagt accaaaagcc aagggctccg cgcgagtcgc cagccggtat
      901 tcacgcccgc cgcgaagcct tcgttttgcg accggaccac caaccgcagc ccggacggag
      961 ggttcaagag cgcggaagga atcggtcccc gcgaggcgtt gtcgacgacc acgatccggc
     1021 attgacccga cgagaactcg ggctcggcga tgagtgatgc agtcaggcgg accgcgtccg
     1081 tccagctttc gtggttgacc acgataaccg tcagccgggc gtgctggctg tcgtggaagg
     1141 ccgggtgttc agacacaatc gcctcttgca cgcggtcgac cccaaaaacg cacggcccga
     1201 atcgtgagat taactctcat tttcggaata tctcaaattt aacgtcggtt cggtcgatcg
     1261 aagagcttga gggaaaaccg aatcgagcga gggctgccaa ggatggtgag tcccaggatc
     1321 gaagacgccg tgccggagcc gattgacggc tcggctgtcg tggcctcccc gaggctcaga
     1381 ccgagggatc ggtggcgatc catgaacagc cctgtggaca tactgaggta tcttgccttg
     1441 ccttcggttg cagccgcgcc ctcgagccgt gtgctcttgg tgggagcccg ccgtgacgcg
     1501 gagcgcttga tacaccggct cgcgaacggc ccatggaacg ggccgcgcat cgtcggattc
     1561 gttgccgcgg gccacggccg attctcgtcg ttgcgaccgc ataaccggca tatcgctctc
     1621 catcctcaaa ccgatcctgt gcccgtcctt ggttcaattg atcgcttgag cgagctagtt
     1681 gaccgcgccc gggccacgca cgtggtcgtc gctgtttcgg gcactcccgg ctaccatatg
     1741 ccgccgccgg tgacccaagt tatcaattcc gacgtgattg tgcagtggat cttggtcgat
     1801 tccggccgtg tggacttagg ctcgctgacg agctcgcctc caaccaagac ctggtcggtg
     1861 cacgctgtcg ggttgccagg aattcggtcc ggaatcccga tctgggctcg cctcaactgg
     1921 gcgcgctcgg ccaaacggac gatggatttc ctgatcgccc tcttggctct ccttgcactc
     1981 gcgcccttgt tcaccgtggt cgcagtttcc atcctggtca catccggtcg accgatcttc
     2041 tacactcaag atcgagtggg acagggaggg agacttttcc ggatcgtcaa gttccgaagc
     2101 atgaagtgcg atgccgagag cgagacggga ccgatctggg cgtccgatca tgataagcga
     2161 tgtacccgga tcggcgattg gctgcggcac tccaacatcg acgagttgcc ccagctctgg
     2221 aacgtcctca caggcgacat gagtctggtc ggaccgcggc cggaacgccc tagcttcgtc
     2281 cacgaattcc gccagacgat tcccaaatac gacttgcgtc acgccgttcc cggcggaatg
     2341 acgggctggg cgcaggtcca tggttggcgc ggtcgaacgt cgctgcgcaa gcggatccag
     2401 tacgatctgg attacatcga gcgctggtcg ctgggattgg acatgcggat cttgctcatg
     2461 accgttcagc acgtattgtg gggcaagacc tcgtggaacg aatcgaagcg gccggcaaaa
     2521 accatggggt gaccacggtg atcggcccga tcgatcgagc catcgcaagg cccgtttgtt
     2581 cggtcgttat accgtcgtac aacggccgcg cgtttctgga gacgtgcctc gcgagcgtcg
     2641 cccgtcagcg tccccgtaat ccggccttgc ccatcgaggt catcgtggtc gacgacggct
     2701 cgaccgacga gacggccgag tggctggcgc gcgttcatcc cgacgtccgg gtggcgagat
     2761 tcgagcgcaa ccgaggtttt tgcgccgctg ccaacgccgg aatcgccagg gccaacggca
     2821 gtttcatcca gttactcaac aacgacaccg aggtcgcggc cggctggatt gaggcaggat
     2881 tagaaccctt cgtcgatccg acggttgggt cggttgcacc gttggtcttg gttcggtccg
     2941 agcccgaacg ggtggactcg gccggcgatt cgtactccct cttcggctgg cccgcgaagc
     3001 gcggtcacgg ccagccaacg caactctttt ccgaacgacc gatggaagaa gtattcggcg
     3061 caagtggatc cagcgcgttc taccgcacga cggcgctaaa gcgatcgggc ggattcgatc
     3121 cgcttttcgg gtcgtattac gaggatgtcg atctggcgtt ccggttgcgc tgggccggct
     3181 atcgctgtgt attcgcacca cgatcgatcg tctaccacga tgtctcgtcg acctatgacc
     3241 acggcagcca cgcgctccac cgccgcatgt cgcgaaacgc cgagttcgtc ttttgggcga
     3301 atctgcccgc ggtgttgttg gggctggcga tcctgcccca tctggtgttt ctggtcgtgc
     3361 agggagcctg gaggctcttg cgcggccgac tgcgcccatt tctggcagga aagtgggatg
     3421 cgcttcgcgc gtggcaggac atcaaggcga ggcgccggca tcgcgccgag ctttgtcgca
     3481 aggcagtagc gacgcctcgt tttgccctgg ggaaaggctc atggcgcgac gtgcgcaatc
     3541 atttaagccg gccgcgcgag caaaccaggg ggcatgcagc gtcactccgt gccaggtgat
     3601 tttcacctgg tgctcctccc ggtatattga tcgatggccc gtcctggcat cgctttcttt
     3661 agatcggcat cagaggcacc ttctttgtgg gcgcggattc ttgaggttct ctttggaatc
     3721 ctgaacgccg ggaaggcgaa cgcagttccg cggctgctgg gcggctactg gctctcgagc
     3781 gagctcggcc ccggacggat cggcccgact tacctggcaa ttcagctcgt gcaaggccgc
     3841 aaggtcgcct tgaagtcgat gcagccgcgc tgggccggcg acgccacttt cgtggctcga
     3901 tttactcgtg aggcctatgc cgctctgcag atcacggacc acaatcttgc ccaaatccac
     3961 gatttcggcg atgaacgtgg aattccctat ttcacgagcg agattgtcga tggacagtcc
     4021 ctgaacgcct tgattcgcgc aaagggccgg ctcgatatcg aaaaggccgt ggtttatgtt
     4081 ctccaggcgg cccgcgggct ccggtgcgcg caccagcagg gtattctcca tcgcgatatc
     4141 aaaccgcaaa acctgctctt gaaccggcag gggctgatca agctgtccga tgtggggctc
     4201 tcgaagacac ccgagtcggc cgaggctgac gacacggtcg gaacgccgac ggcactcaga
     4261 atcgacaccg gtgccgacat ctgttcgttg ggatgtactc tgtacgccct gttgacagga
     4321 atgcagcaag tcgagtccgc cactgtcgca gacatcaagg gcggtcagcg gacgcaaccg
     4381 atcaaccctc cccatccggt cggcgaagtg ataccccaag cactgtcggc catcgttcgc
     4441 aagatggttg ccgcccagcc cgaggatcgt tacgcgagtg cgagcgatgt gatcgcagca
     4501 ctggaaacgt ttttgggcat ttcgtcctca cttccattca acccgctcga cgagcatgcc
     4561 agcttgctcg accaatccgt cagagcgtat cacggctcgc agacggcgct cttgcgttca
     4621 agggtcaagt cgctcttttt cggcgcgtgc ttggcgctgg ccattctctg cctgatctcg
     4681 gcccgtccct tagcgggagt cgggttcgcc agtctgggtt ttctgacggc tcttgccgat
     4741 ttcgtgctgg ccgggatccg gcggcagagc gcgctctttg tcaagcttcg cgagttgatc
     4801 gcgaccgagc gaatgtcgga atggctcacg gccctggccg cgctggcgat tttgatcgtg
     4861 ctgctcatga tcctgaagct gttttggctc ttgctgggcc tcgggtttct cgcgatcggc
     4921 attgtcgcgg cattacatgc cgcgttcgac cgccggattg acgccgacag agccaaatcg
     4981 ctcgccgagg tcgagcggat gctgcggtcg ctgcgtctga acggagtcga tgaagacgcg
     5041 cttcggcggt ttgtttggat tcatggcgga acgcacgcgg aggagtttta cgaggctctc
     5101 ttcggctacg acgagaaacg ggaagcgcgt cggcgcttta atcgaagcga acgatcgggc
     5161 cctcggtcca agtttgcgtc gtggcgggac ccgatcgccg actggctcga cgccacggtc
     5221 gccgcgcgac gagcggccgg cgagatcgtc aagctgcaac ggattgaaga gaagagcctg
     5281 gaaagcctgg gcgaaaacct ggtgacggcg cgccgtaagg gccagcggtc ggcggaggcg
     5341 atggtggcgc tagccgcgga gatccggaaa acgaaccgcg ctggcgacgg cgagtacgcg
     5401 gtcaatcact cgatcgccga agccatgcgc gaggccgccc tcaagccgga gaaggtgctg
     5461 gtccaacgcg agcgcggcct gctgccggaa cggcggcgat cggtcggtct cgcaaccatc
     5521 gtcaacatga tcgtcggtcc caatgtgcgg ttcctgttcg gggcagcgct ccttgccggc
     5581 tgcatcgcct ggatgcatca gaacgcgatg atctcgccgg aacatgcggg ggccctggtc
     5641 gaggcggcga agtctggcga tgtagacgcc atccggtcgc acgcgcaggc ccaagtggcc
     5701 cacgcccgtg aggctgccgc caagcggacc gatccgctcg ctttgccagt cgtgcccgcc
     5761 gcgctgctca atctggtctc atcgtttggc gccggcgtca gtgggctctt cctgattgct
     5821 tcgtcggtct ttggtggccc caggattgca cttttcgcca tccccgcagc cgcggtccca
     5881 ctcctgggcc cacagcttgg cctgccgtct atgggcggac tcgagccgag cgtcgtcccc
     5941 atcattatcg gactggcgct catgatcgca ggagtcgtgt tcggccgcga atgatttcgg
     6001 gctcggcaac attgaccagt ttttttcgag tccggccgat cattccccgg ccaaccccag
     6061 cgttcgcgcc gctcgaggtt cggccacggt caagtcagtg cgaggaaggg tcatctcgtc
     6121 caagatcgtg cgtcggccgt agttgtcgta aaacgttccg tgcgtttcaa cgacctggtc
     6181 gcgatagtcg gcatgaaacc acttgagcag ctcgtgcagg cgccgtagcc ggtaatgcgg
     6241 cacagccgga aagagatgat gcggaatgtg catgtcctgg ccgtagacaa gaacggccca
     6301 ccgggtaaat gggtcggtaa agaagacccg cgaattggtc aaccgtcccg agtcggcatt
     6361 ggagtgctga tagacatctc gcaagaacat ataaaacata aacgagctac caatcggcac
     6421 cagccagagc agcacggggt aaatcgccga cctgccgcct gtgcaccacc ggagataggc
     6481 caggcctacg agtacgaagg tgtagtaggc cattcgcaaa aatccggcaa tccggggcga
     6541 gtaggcttgc cggaacggcg actgaaacac ggcccaatcg ggcagggccc gaatgacgcc
     6601 ggcgaggatc gcaatccctg ccagcccggc cggggccagc caatgcgggc ggcccgttgc
     6661 cgtcaggagt ttcagcgcca ggccgaatcc cacgatgtac gcaaaaccca gcaccgtcgc
     6721 cagccgcggc aacgattcag ttgcgctgcc ggcaccggcc gcgcgctcga tatagacgct
     6781 cttgcccttg ccaagcgtgt tcactgtgaa ataggcccac tgatagcgga ggaaccggac
     6841 cgggtccacc aagaaacaga agtaaataac cgtgataaag cgatgccgcg gcatcggaaa
     6901 ctcatcggcc cgtttgccat ggcccaagtt ctgcaggtcg ggatctcgag ctgggtcgtt
     6961 tgtgaattgg tggtgcccca tgtggaacag gcgatagaaa tggatcgtcg tcatcaggtg
     7021 aaacatgcaa aacaggtccg gaatcaggtc gttaagcgt
//
LOCUS       DATAQL010000590         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_212581, whole genome shotgun sequence.
ACCESSION   DATAQL010000590 DATAQL010000000
VERSION     DATAQL010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212581"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            719..958
                     /locus_tag="VHS97_10310"
     CDS             719..958
                     /locus_tag="VHS97_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448636.1"
                     /translation="MTTTVWGVVREGRVVPSSPLPEGARVEIRLADPLPDVPEGLQSE
                     FEAWGRASDKALDLVETLAEERIYCAPGGSVWIKR"
     gene            complement(976..2301)
                     /locus_tag="VHS97_10315"
     CDS             complement(976..2301)
                     /locus_tag="VHS97_10315"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEX3448637.1"
                     /translation="MWLVRGLAEAFLSGSWELDGLVKRGGLILGRRYRWLRPLARRVL
                     ESFPDGARPRVARLADFLWADERFRNEWRKHGPVLHFKKWPVPGMAPPPGPPASWPVP
                     AITSPGDLARYMELEPNELDWFADCQTRERSAAAEPLRHYRCRWVAKRSGSLRLIEAP
                     KPRLKQLQRRLLDEILVHIPPHDAAHGFRPGRSVTSFVAPHVGRTIVLKMDLRDFFVS
                     ITSARVMAIYLTAGYPEPVARLLTGLCTNTVPLAVWNQTEPRLLDPARPAASWQARRL
                     YRQPHLPQGAPTSPALANLAAHRLDARLAGLARAAEASYTRYADDLVFSGGESFARSI
                     ARFPVHAAAIALEEGFAVQHRKTRVMRQGVRQRAAGVVINQKPNITRDDYDQLKAILH
                     NCVCQGPGEQNRAGVADFRAHLAGRVAYVGRLNPERGEKLSRVLERIVW"
     gene            complement(2572..>3316)
                     /locus_tag="VHS97_10320"
     CDS             complement(2572..>3316)
                     /locus_tag="VHS97_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3448638.1"
                     /translation="YRPSPQKFPGPRPVLIDIHGGPEGQFRPSFLGRLNYWINDLGIV
                     LIFPNVRGSSGYGKTYLKLDNSMKRDDSVKDIGALLDWIAAQPDLDKARVGVTGGSYG
                     GFMSLAVQTTYNDRIKAGIDIVGISNFVTFLKNTQGYRRDLRRAEYGDERDPQMRSFL
                     ERVSPLARAHMIHTPILVVQGQNDPRVPISEAEQMVSALKKNGIPLWYVVGTNEGHGF
                     AKKINQDYLQAVEVIFLRHFLLGEESLKR"
BASE COUNT          671 a          986 c         1047 g          612 t
ORIGIN      
        1 aaagaccatc gcctcatcaa tagggggagc cggcacgacc atcttcgggt cgattgttgc
       61 gtccgcgacg atcacgaacg gatcgggtag ttggctcgcg attgggaggg cgggcggatt
      121 cataacaggg actcgagctc cactgattcg ttgtttcggg gtgattctat gccctacggt
      181 cccagcgata atcgccatcg tcaaggcgct cgcggccacc acgagcatta gccgcccaat
      241 cgaacggacg ggccaccacc accgagggaa cttcagcctg atctcgtcat cgaaacctgg
      301 attgggtttc acgggacgtt cctttctcca aaatccgtgc gggtgtatcg ttcgatgact
      361 ccagcccaga attacgtcaa cttgatagcg gccgacatcg gtttttctta ggccaacacg
      421 gcccggatca tcttagcatg gacgtcggtg aaacagcgcg atgtgctggg ctttcgcggc
      481 aaaatgcaga gtttgggagc gaacggctta atcggcgtac gcccggccga gttgtgtgtc
      541 ggcgtgttgg ctgtgccatc gttgtcggcg gacattgccc atcctgcgcc agcagtgcac
      601 ccagcgcgat tccccatgcc tgtagccaaa tgcgccataa acttgcggcg gtctagcagg
      661 tggcgggcca gaagtattcc ggctatgata atagtagtta atccaggagc attccgccat
      721 gacaacgacc gtctggggcg tcgtaagaga ggggcgcgtg gtgccaagct cgccgctacc
      781 cgaaggtgcg cgcgttgaaa tccgactagc ggatccgctg cccgacgtcc cagagggttt
      841 gcagtcggaa ttcgaggcgt ggggtcgtgc cagcgacaag gcattggatc tggtcgaaac
      901 tttggcggaa gagaggatct actgcgcgcc gggaggcagt gtctggatca agcgataatc
      961 atgtagaaaa atcaatcacc agacaatacg ctcgagaact cgcgagagtt tttcgccgcg
     1021 ctcgggatta agccgaccga catacgcaac gcggcccgcc agatgcgcgc gaaagtcagc
     1081 cacgccggcg cggttctgct cgccgggccc ctggcacaca cagttatgca gaatcgcctt
     1141 caactgatcg taatcatctc tggtgatatt aggtttctga ttgatcacga ccccggcggc
     1201 gcgttgcctg accccctgcc gcatcactcg tgtcttgcga tgctgcacgg caaatccttc
     1261 ctcgagcgca atggcagcgg cgtggaccgg aaaccgggca atcgaccgcg caaacgactc
     1321 gccgcccgag aaaacgagat cgtccgcata ccgggtgtag ctggcctcgg cggctctggc
     1381 cagaccggct agccgcgcgt cgagccggtg ggcggccagg ttggccaggg ccggtgaggt
     1441 tggggcgccc tggggcaggt gcggctggcg gtagagccgc cgggcctgcc aactcgcggc
     1501 cggacgggct gggtcgagca ggcgcggttc tgtctggttc cagaccgcca gaggaactgt
     1561 gttcgtgcaa agacccgtta gcagtcgggc caccggttcg ggatagccgg ctgtgagata
     1621 aatcgccatc acccgggcac tagtgatgga aacgaagaag tcgcgcaaat ccatcttgag
     1681 cacaattgta cggccgacat gaggcgcgac gaacgatgtg accgatcgac ccggccgaaa
     1741 gccatgggct gcgtcgtgcg gtgggatgtg caccagaatc tcgtcgagca accggcgctg
     1801 tagctgtttg agccgcggct tgggcgcttc gatcaggcgg agcgacccgg atcgcttggc
     1861 cacccagcgg caacgatagt ggcggagtgg ctcagcggcg gcggaccgtt cgcgcgtctg
     1921 gcagtcggcg aaccagtcga gctcgttggg ttcgagctcc atgtaccgag ccaggtcgcc
     1981 cggcgatgtg atcgctggaa ccggccaaga tgccggcggg ccaggcgggg gtgccatgcc
     2041 gggaaccggc catttcttga aatggagcac cggcccgtgc ttgcgccatt cgttgcggaa
     2101 ccgctcgtcg gcccacaaaa aatcggccag tcgggctacg cgcggtcgag caccgtctgg
     2161 gaacgactcc agcacgcgcc gagcaagcgg ccggagccag cgataccggc ggccaagaat
     2221 cagaccgccc cgcttgacca agccgtcaag ctcccaggaa cctgagagaa acgcctcggc
     2281 aagaccccga accaaccaca agcgtgcgcg attgatcgaa gacagtggtg gcggctccaa
     2341 cccgtcttgt ccggcgtgag gagtcgtgcg cggacagcgc acccggcctc acgcgctgaa
     2401 atgatccggt caccgtggtc cccggggtaa ccccggagcg tcgagccgac cgccaaagac
     2461 ggcctctcta ctcgcttgga gcaccgccac cactaggcga aatcataagg cgatatcgat
     2521 acttatgtca agtaggagcg ggttggttgg cgcagccgat cgtaacgcgc tctatctctt
     2581 gagactctct tcccccagca ggaagtggcg gaggaatatc acctccacgg cttggagata
     2641 gtcctggttg atcttcttgg caaagccgtg cccctcgttg gtgccgacca cataccagag
     2701 cgggatacca ttcttcttca atgcggagac catctgctcg gcctcagaga tcggcactcg
     2761 cggatcgttt tggccctgga cgaccaaaat cggcgtatga atcatgtgag cacgagcgag
     2821 cggcgaaact cgctcgagaa acgaccgcat ctgcgggtcg cgctcgtctc cgtactcggc
     2881 gcgccgcaga tcgcggcgat acccctgagt gtttttcaag aatgtcacga agttggagat
     2941 gccgacgata tcgatgccgg ccttgattcg atcgttgtag gtcgtctgga cggctagcga
     3001 catgaagccg ccgtaggatc cgcccgtcac ccccacgcgt gctttgtcga gatcgggctg
     3061 ggcggcgatc caatcgagca aagcgccgat gtctttgacg gaatcatcgc gcttcatgct
     3121 gttgtcgagc ttcaaatatg tctttccata accggatgag ccgcgcacgt tggggaagat
     3181 caagacgatc cccaggtcgt taatccagta attcaggcgc cccaggaatg acgggcgaaa
     3241 ctgaccttcg ggcccgccgt ggatatcgat caaaaccggc cgggggccgg gaaacttctg
     3301 gggcgacggc cgatac
//
LOCUS       DATAQL010000591         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3417737, whole genome shotgun sequence.
ACCESSION   DATAQL010000591 DATAQL010000000
VERSION     DATAQL010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3417737"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..2571)
                     /locus_tag="VHS97_10325"
     CDS             complement(433..2571)
                     /locus_tag="VHS97_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEX3448639.1"
                     /translation="MTMHAMPVMLGVLAILAVAYRYYSAFLAARVAVLNDEQATPAHR
                     YADGQNFDPTNRWVLFGHHFAAISGAGPLIGPVLAIQFGYLPGLVWLVVGVCLAGAVQ
                     DMMVLAASTRRGGMSLAGLAREEISRPAGLAAAIAILYIVIIALAGLGIVVVKALGGE
                     EVPMKAGTTLVYADNSKINRTFFPDRPTIHKIPPGSTYRFGSGPSQSMLINEGFDLTV
                     PHNARLSLGPDGKGWIIPEDAKRLVKGSSWGTFTIACTIPIALFVGWYMYRLRKGKIL
                     EASLLGAAAVLAATVGGAFIPGSELEKYFSLSREAAVFALAAYGFVAAVLPVWLLLAP
                     RDYLSTFLKIGTIFLLVIGVVIVNPKLQAPAVSPYFAAGGGPYFDGPIFPYVFICIMC
                     GAISGFHALVSSGTTPKLIDRESDVRMIGYGAMLMEGLVGVVALIAAAALPNAMYYDI
                     NIDLSRRADFMASHRDFAQFLQAGEVDPHGGHGAGAPGGSELVAMERDVQESLHGRTG
                     GAVTLAVGMARIFSNAIPGMNWLIKYWYHFAIMFEALFILTTIDAGTRIARFLVQEFL
                     GRIWKPLGDLNWLPGSLLATGLVVFGWGYFIYTGSVETIWPMFGMANQLLAVIALAVV
                     TTYLVNSGRGRYAPVTVLPMLFVATTTTTTAYYEITGKFWNMVKGVAPVRGWLNIGLT
                     GMLLACVAVIVISAVVRWVSPSTRAPALDS"
     gene            complement(2608..3198)
                     /locus_tag="VHS97_10330"
     CDS             complement(2608..3198)
                     /locus_tag="VHS97_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448640.1"
                     /translation="MAKPLSTRRRSTSPPKADYQLLGPMPARMYEVILPKKLGYFGKV
                     QEVLEDLFNEQAIREVPFIQQSIADRRGQYAEFDEDGWIKTLCQASGGYSIYEMDGRY
                     LSPQGPVDERVLVIRFIFHNPSDPADSRTDFLAASLEVVNYLVAHRFANELGVEEEIW
                     FLEYHLPRLAIWRKGPGHPPAESVSERENRGSKPGK"
     gene            complement(3191..>4005)
                     /locus_tag="VHS97_10335"
     CDS             complement(3191..>4005)
                     /locus_tag="VHS97_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448641.1"
                     /translation="SIGGTSVTAGQDLFSVFEPARGQHPARDTATMPTLDDSFEAVHT
                     AVLDRFGPPPTEFEGLDPFEAMVAVLLNRATGGMGSKAGLEDLGGDGLLTPEGLAKAE
                     IPEIMDAIHGHGVSAPARSVAPLRQLARWLVDHHEARVDSLFDPHRSADTLRGELAGL
                     NGISVSGADALILYALKKPSYPVDRATFRVLVRHDWLEPTATYEEARDLIVDHALKEA
                     DLREGDAASLLAELAHGMQQLGRRFCRTARPLCDGCPLESVLPEGGAREIDG"
BASE COUNT          845 a         1247 c         1208 g          705 t
ORIGIN      
        1 gcgatcccgc gggacctgcc gagaaacgcg tccgtttact ggaacaggct aagcgaactc
       61 aactagacgc atcacgacca ctaaaggaac ccgccgtgca gatgcgaagt gaaccacagt
      121 agcgatcttc agtcccgatg ccgttgaaat agcaacaaat cacgagtgca taagaacgta
      181 taatcccctc cccccttgtg gggtagggta agggtggggg gcttcccgtc acgtaccgct
      241 tccaagccta ttttctggcg gtggcctcct cccaccccaa ctctccccca caagggggga
      301 gggagacaaa gcggaattcg ggctaagaag ttacatccga ttcaatggtg ttggcgttag
      361 gccgcggaag atagctgacg aatggtggcg ttgattgggg gtcgaaatca tgcaaacaga
      421 atccgccagc cgctacgaat ccaatgccgg agcccgcgtc gacggagaaa cccaccgaac
      481 caccgccgag atcacgatga cggccacgca cgccaaaagc attccggtga gcccgatatt
      541 gagccagccg cgcacgggtg cgactccttt gaccatgttc cagaacttgc cggtgatctc
      601 gtagtacgcg gttgtggttg tggtggtggc gacaaagagc atgggcaata cggtaaccgg
      661 tgcgtaccgg ccgcggcccg agttgacaag gtaggtcgta accaccgcca aggcgatgac
      721 cgccagcaac tggttggcca taccaaacat cggccagatc gtttcgacac tgcctgtgta
      781 gatgaaataa ccccagccaa agacgaccag gccggtcgcc agcagtgagc ccggcagcca
      841 gttcaggtct cccagcggct tccagatcct gcccagaaac tcctggacca ggaaccgcgc
      901 gatccgcgtg ccggcgtcga tcgtggtcag gatgaacagc gcctcgaaca tgatcgcgaa
      961 gtgataccag tacttgatga gccagttcat gcccggaatg gcattggaaa agatccgcgc
     1021 catgccgacg gccagcgtga cggcgccgcc ggtgcggccg tggagtgatt cttgcacgtc
     1081 gcgctccatc gcgaccagct cgcttccgcc tggggcgccc gcgccgtggc cgccgtgcgg
     1141 atcgacctcg cctgcctgaa ggaactgggc gaaatcacgg tggctcgcca taaaatcggc
     1201 ccggcggctc aagtcgatgt tgatgtcata atacatcgcg ttgggcaagg cggccgccgc
     1261 gatcaaggcc accacgccca ccagtccctc catcaacatc gcgccgtagc cgatcatccg
     1321 cacgtcgctc tcgcggtcga tcaacttggg cgtagtgccc gaagagacca gggcgtgaaa
     1381 tcccgagatc gctccacaca tgatgcaaat aaaaacatag ggaaaaatgg gcccatcgaa
     1441 ataaggcccc cctccggcgg cgaagtacgg gctgaccgcc ggtgcctgaa gcttgggatt
     1501 cacgattaca accccgatga ccaagaggaa gatcgtgccg atcttgagaa aggttgacag
     1561 gtaatcgcgc ggagcgagca agagccacac cggcagcaca gccgcgacga agccgtaggc
     1621 cgccagtgca aacacggcgg cctcgcgcga gagtgagaaa tatttttcga gctcggagcc
     1681 gggaataaaa gctccaccaa ccgtggcggc cagcacggcg gccgctccca gcagtgacgc
     1741 ctcgagtatc ttgcccttgc gcaggcggta catgtaccac cccacgaaaa gcgcgatcgg
     1801 gatcgtgcaa gcgatcgtga atgtgcccca tgagcttccc ttcacgagcc gcttggcatc
     1861 ctccggtata atccagccct tgccgtctgg gccgagcgac aatcgtgcat tgtgcggcac
     1921 cgtcagatcg aagccctcat tgatgagcat gctctggctc ggtcccgatc cgaagcgata
     1981 agtcgagccg ggagggatct tgtgtatggt tggtcgatcg ggaaagaacg tcctgttgat
     2041 cttggaatta tcagcgtaca cgagcgtagt cccggccttc atggggactt cctcgccgcc
     2101 gagcgccttg acaaccacga tacccagccc cgcaagcgcg atgatcacga tgtagagaat
     2161 cgcgatcgcc gcggccagcc cggcagggcg gctgatttct tcgcgtgcca gtccggccag
     2221 gctcatgccc ccacggcggg ttgaggcggc cagaaccatc atgtcctgga cagcgccagc
     2281 caagcagacg ccgaccacca gccacaccag gcctggcaag tagccgaact ggattgccag
     2341 caccggaccg atcagcggac cggctcccga gatcgcggca aaatgatggc cgaagagaac
     2401 ccagcgattg gtcgggtcga agttttggcc gtcggcgtag cggtgcgccg gagttgcctg
     2461 ctcatcgttc aaaaccgcga ccctggcggc cagaaacgca ctgtaatacc gataggccac
     2521 cgcgaggatc gccaggacgc ctagcatcac cggcatcgcg tgcatcgtca tcgcgcaaca
     2581 acctccaccg ctcgttcgag cgctcgttta cttacccggt ttagagccgc ggttttcccg
     2641 ctcggagacg ctctcggccg ggggatggcc cggcccttta cgccaaattg cgagccgcgg
     2701 caaatgatat tctaggaacc agatctcctc ttccactccc agttcgttgg cgaaccggtg
     2761 ggcgaccagg tagttgacaa cttcgagcga ggcggccagg aaatcggtgc gcgagtcggc
     2821 cgggtcgcta ggattgtgaa agataaatcg gatgaccagg actcgctcgt cgaccgggcc
     2881 ttgcggcgac aggtaacgcc cgtccatttc atagattgaa tagccacccg aggcctggca
     2941 aagcgttttg atccagccat cctcgtcaaa ttcggcgtac tgaccccgcc gatcggcgat
     3001 cgactgctga atgaaaggga cctctcgaat cgcctgttcg ttgaagaggt cttcgagcac
     3061 ctcctgaacc ttaccgaagt aaccgagctt cttgggcaga atgacttcat acattcgcgc
     3121 gggcatcgga ccgagcaatt gatagtcggc cttgggcggg gaagtcgatc ggcgcctcgt
     3181 ggacaggggc ttagccatcg atttcgcgcg ctcccccctc aggcaagacc gattccaagg
     3241 gacagccgtc gcagagtggc ctggccgtgc ggcagaatct acgcccaagc tgctgcattc
     3301 cgtgcgccag ctcggccagc aggcttgccg cgtccccttc tcgcagatcg gcttctttga
     3361 gggcgtgatc gacaatgagg tctcgcgctt cctcgtaagt ggccgtcggc tcgagccaat
     3421 cgtggcgaac caagactcgg aaagtggcgc gatcgaccgg gtatgacggt ttcttgagag
     3481 cataaaggat caatgcgtcg gccccgctga cgctgatgcc gttcaagccg gcgagttcgc
     3541 cgcgcagcgt atcggccgac cggtgcggat caaaaagcga atccacgcgg gcctcatgat
     3601 ggtccaccag ccagcgcgca agttgcctga gcggggctac gctccgcgcc ggtgctgaca
     3661 ctccgtggcc atggatcgcg tccatgatct cggggatctc ggccttggcc aacccttcgg
     3721 gcgtgagcag gccgtcgcct cccaggtcct cgagtcctgc tttcgagccc attccgccgg
     3781 tcgcgcggtt caatagcacg gcgaccatgg cttcgaacgg gtccaagccc tcgaattcgg
     3841 tcggcggcgg gccgaaacgg tcgagcaccg cggtgtgaac cgcttcaaaa gagtcgtcga
     3901 gggtcggcat ggtggcggta tcccgcgcgg ggtgttggcc tcttgccggc tcgaagaccg
     3961 aaaagaggtc ttggcccgca gtcacgctcg tgccgccaat cgatc
//
LOCUS       DATAQL010000592         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2808614, whole genome shotgun sequence.
ACCESSION   DATAQL010000592 DATAQL010000000
VERSION     DATAQL010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808614"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VHS97_10340"
     CDS             complement(<1..884)
                     /locus_tag="VHS97_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEX3448642.1"
                     /translation="MNRCNETLAAVSHFVHKNLLWLMLGSYATAALFPAAGLAIRDAS
                     IHGIGPLGAGTKISVSMIMLAALLLNAGLGVRRNQIRDMVRSPRILGLGMASNLVIPI
                     AFIFVVSRFMSGWHNADEVQNILVGLALVASMPIAGSSTAWAQNADGEAALSLGLVLF
                     STLLSPLTTPVALHSVGLMAAGDYAEDLHELASAATGTFLVLGVITPAFLGIAIGWVV
                     GEARLNAARPGLKLINSIVLLFLNYSNASVSLPQAVADPDTDFLAATLAIVAGLCVLA
                     FGAGWLISHLIGATPSQRT"
     gene            complement(1023..1352)
                     /locus_tag="VHS97_10345"
     CDS             complement(1023..1352)
                     /locus_tag="VHS97_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX3448643.1"
                     /translation="MIDSPLPPDPLVQHWLKRHQSYASFILHMIGIPPTILGVLFVSI
                     YLTLLSLPIFFVALALFVGGYLLQFAGHAIEGTDPGEIIYFKRKLGLPYVEFPPGRSP
                     EKADAPA"
     gene            1555..2649
                     /locus_tag="VHS97_10350"
     CDS             1555..2649
                     /locus_tag="VHS97_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX3448644.1"
                     /translation="MAHDVTLILGDGVGPELAEAAKMCVEATGVAVNWDEQEAGIDVM
                     AKLGTPIPDSVLESCRRTKVALKAPITTPVGTGFRSVNVYLRHALDLYACVRPCKIYP
                     GVRTTFSDTPVDLVIVRENSEDLYFGCEFEQGKPETAELIADIKRLNGKAIRPDSGIS
                     IKAISIFGTRRIVKYAFEYARKHGRKKVTSVHKANILKFSDGLFLEISREVAKDYPDI
                     EFEDRIVDNMCMQLVQKPELYDVMVLPNLYGDIISDLCAGLVGGLGVAPGANIGEAGA
                     VFEATHGSAPKYKGLYKMNPTALILSGVLMLEHLGEKVAASRLEKAVAKVIKDGKDVT
                     YDMKPNRNDPTAVGTLDMAKAIIRAMEAGV"
     gene            2646..3701
                     /gene="lpxK"
                     /locus_tag="VHS97_10355"
     CDS             2646..3701
                     /gene="lpxK"
                     /locus_tag="VHS97_10355"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX3448645.1"
                     /translation="MSRFDRTWYLRLIRGQNSGLWPPLALAGLRAASVGYGAAVAVRN
                     AGFDRGWTKCHRALVPVISVGNLTLGGTGKTPMVEWLARWYRRRGLRVAILSRGYGQD
                     GGLNDEGQVLEENLPDVPHLQGPDRALLAGIAAHELETELILLDDGFQHRRLARDLDL
                     VLIDALEPFGLGHLFPRGLLREPIASLRRASVVVLSRADLISAAEKEAIRAKILLKAG
                     RIPWVEARHAPLDMIDGEGIASPLELVQGQSIAAFCGIGNPEGFRRTLLPLCRELRDL
                     RVFPDHHIYSAEDVRSLERWAAQVGANLVLTTQKDLVKLRAGLLGPAPLRALRIGLEL
                     TAGGDIMDDVLARLLST"
     gene            3805..>4110
                     /locus_tag="VHS97_10360"
     CDS             3805..>4110
                     /locus_tag="VHS97_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX3448646.1"
                     /translation="MKIAVFCPNLIGDTVMATPTFRALRAGFPCATIVGVIKPRVAPT
                     LDATPWFDDWIYFDRRSSQRVERTTAVVRRLRRERFQLAVLLPNTFRSAWMAWLAQIP
                     "
BASE COUNT          859 a         1177 c         1297 g          777 t
ORIGIN      
        1 gttcgctggg atggagttgc cccgattaga tgcgagatca accaaccggc cccgaatgcc
       61 aacacgcaca gtccggcaac gatcgccagc gtggcggcca ggaagtcggt gtcgggatcg
      121 gcaacggcct gcggaagtga aaccgacgcg tttgagtagt taagaaaaag caggacgatg
      181 gagttgatca atttgagacc aggccgcgct gcattcaatc gcgcttctcc aacgacccag
      241 ccgattgcga tacccaggaa cgcgggggta atcacgccaa ggacgaggaa tgtcccggtg
      301 gcagccgagg ccagttcatg aagatcttct gcgtaatctc ccgccgccat caatccgacc
      361 gaatggaggg cgaccggagt cgtaaggggg gacagcaggg tggagaacag gaccagcccc
      421 aggctgagcg ccgcttcgcc gtccgcattt tgggcccaag cagtcgaaga gcccgcaatc
      481 ggcatggaag cgaccagggc caatccaaca aggatgttct gcacctcgtc cgcattgtgc
      541 cagccactca tgaaccggct aacaacgaag atgaacgcga tcgggatgac gagattcgac
      601 gccatcccca gtcccagaat cctcggagag cgaaccatgt ctcggatctg gttgcgccgg
      661 acgcccagcc cagcgttcag cagcaaggcg gccagcatga tcatcgatac gctgatcttg
      721 gtaccggccc cgagagggcc gatgccgtgg atcgacgcgt ctcggatcgc cagacccgcc
      781 gccgggaaaa gcgccgcggt cgcgtacgag ccaagcatga gccagaggag gtttttgtgg
      841 acgaagtggg aaaccgcagc caatgtttcg ttacaacgat tcatccgagg tatccgtacg
      901 ggatgcgatc gcgctaaagt cgcccgatgt cagagcggca ccggctgacg gccaggcggt
      961 gccggcatca tagtgaggca acctaagcag accttaaagg gcagataaga acttgttaac
     1021 gatcaggccg gagcgtcagc cttttctggc gaacgtcctg gcgggaactc gacatagggc
     1081 aaaccgagct tgcgcttgaa gtagatgatc tcgcccggat cggtcccctc gatggcgtgg
     1141 ccggcaaact ggagcaggta tccgccgaca aatagtgcca gtgccacgaa aaagatcggc
     1201 agggaaagca gcgtgagata gatagataca aagagcacgc ccaagatggt cggcgggatg
     1261 ccgatcatgt gcaggatgaa gctcgcgtaa ctttgatggc gcttgagcca atgttgcacg
     1321 agcggatccg gaggcaatgg tgaatcgatc atgaaagtgc atccgggctc tgtgcaaacc
     1381 gagcgaattg caggcagggc cggcggtgct tgacggaccc ccccgcgcca agttaggttg
     1441 gcacttcaag ataccagttc gctggattat cgcaaccttt aggcatttac cggaatgggg
     1501 caagagcgcc cagtctgaag gcgagtgctc gacgcccagg gagattgcag accgatggcg
     1561 catgacgtga cgttgatcct cggagatggt gttggccccg agctggcgga agcggccaag
     1621 atgtgtgtcg aggcgacggg cgttgccgtt aactgggacg agcaggaagc cggcatcgat
     1681 gtcatggcca agctgggaac gccgattccc gacagcgtgc tcgagtcatg ccgccgtacc
     1741 aaggttgcct tgaaggcccc gatcacgacg cccgtcggca ccggtttccg gagcgttaat
     1801 gtctatcttc gtcatgcgct tgacctttac gcgtgcgtcc gaccgtgcaa gatttatccc
     1861 ggcgtcagga cgaccttttc cgacactccc gttgacctgg tcatcgttcg cgagaattcc
     1921 gaagacctgt attttggctg tgagtttgag caaggcaaac ccgaaacggc cgagttgatc
     1981 gccgacatca agcgattgaa cggcaaggcc atccggcccg actcgggaat ttcaatcaag
     2041 gcgatttcga tcttcggtac gcgccggatc gtcaagtacg cttttgaata cgcccgaaaa
     2101 cacggccgca agaaggtgac ctccgttcac aaggccaata tcctcaagtt cagcgacggc
     2161 ttgttcctcg aaatcagccg cgaagttgcc aaggactatc ccgacatcga gtttgaagac
     2221 cgtatcgtcg acaacatgtg catgcagctt gttcagaagc ccgagcttta cgatgtgatg
     2281 gtcttgccca acctctacgg cgacatcatt tccgacttgt gcgccggctt ggtcggggga
     2341 ttgggtgtcg ctcccggtgc caatattggc gaggccgggg cagttttcga ggcaacgcac
     2401 gggtcggccc ccaagtacaa ggggctctac aagatgaatc cgaccgcgct gatcctttcg
     2461 ggagtcttga tgctcgaaca cctgggcgag aaagtggcgg cgagccggct ggaaaaggca
     2521 gtcgccaagg tgatcaagga tggcaaggac gtcacctacg acatgaagcc caatcgcaat
     2581 gatccaacgg ccgtgggcac gctcgacatg gccaaggcga tcatccgggc gatggaagcg
     2641 ggtgtttgag ccgattcgac cggacctggt acttacgctt gatccgaggc cagaacagcg
     2701 ggctctggcc accgctcgcg ctcgcgggat tacgcgcggc gtcagtcggg tatggcgcgg
     2761 ccgtggccgt ccgcaatgcc ggctttgacc ggggatggac caagtgccac cgcgcccttg
     2821 tgccggtgat ctcggtcggc aacctcacac tgggcggaac cggcaagacg ccgatggtcg
     2881 agtggctagc gcgatggtat cgccgccgcg ggctgcgggt ggcgatatta agccggggct
     2941 atggacagga cggagggctg aacgacgagg ggcaggtttt ggaagaaaac cttcccgatg
     3001 ttccccatct tcaggggccc gatcgcgcat tgttggccgg cattgccgcc catgagctgg
     3061 agaccgagct gatcttgctc gatgacggat ttcagcatcg ccggttggcg cgcgatctcg
     3121 atctggtcct gatcgacgcc ctggaaccgt ttggtctggg gcatctcttt ccccggggcc
     3181 tgttacgcga accgatcgcc tcgctgcgcc gtgccagcgt ggtcgtcttg tcgcgcgcgg
     3241 atctgatctc tgcggcggag aaagaggcga tcagagcaaa gattctgctg aaggcagggc
     3301 ggataccttg ggtagaggcg cggcacgcgc cactcgacat gatcgatggc gagggaatcg
     3361 cctctccgct agagttggtt cagggacagt cgatcgcagc gttttgcggc atcggcaatc
     3421 cagaaggatt tcgccgcacc ttgctcccat tgtgccggga gttgcgcgat ctacgagtgt
     3481 tccccgatca tcacatctat tcggccgaag atgttcgttc gctggagcgc tgggcggccc
     3541 aagtaggagc caatctcgtc ttgaccactc agaaggattt ggtcaagctg cgcgccggct
     3601 tgcttgggcc agccccgctc cgagcgctgc gaatcgggct cgaactgacg gcgggaggag
     3661 atatcatgga cgatgtcctg gcccggctct tgtccacctg aattccggac gagccgttcg
     3721 aaaaggattt cttgtgagcc gtcttggatc ggaccaaagg gtagcagccc aggcgcgcgt
     3781 agtcgcgagc aagggttggc taccatgaaa atcgccgtgt tctgccccaa cctgatcggg
     3841 gacacggtga tggccacgcc gacctttcgc gccttgcgcg cgggttttcc atgcgccacg
     3901 atcgtcggcg tgatcaaacc acgcgtggcc cccacgctcg acgcgacccc ttggttcgac
     3961 gattggattt atttcgatcg acgttccagt caacgagtcg agcgaaccac cgcggtcgtt
     4021 cgccgattgc gccgcgagcg gtttcagttg gcggttcttt tgccgaacac gttccgcagc
     4081 gcctggatgg cctggctcgc ccaaattcca
//
LOCUS       DATAQL010000593         6284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5404607, whole genome shotgun sequence.
ACCESSION   DATAQL010000593 DATAQL010000000
VERSION     DATAQL010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5404607"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /locus_tag="VHS97_10365"
     CDS             complement(<1..1940)
                     /locus_tag="VHS97_10365"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3448647.1"
                     /translation="MGRQSTGHYRAKNAIEPDRATLSGDDEIAALRAIVEGTARNTGE
                     VFFESLVRHLARAIDVSYAFVAEFAGSNERVRTLAYWSKGKLRENVEYDLAGTPCEDV
                     VRGGLCHHPLGVKDRFPDDRDLAAMGIESYLGVPLLDGEGTVLGHLAVFDGRPMAPEP
                     RLLFIFRIFATRAAAELERVRVEKQLIESERRYRDLYEEAPNAYVSFDRKFQLLSVNR
                     RAAQLLDHPAAELVGSNVLAHFAATPAGVVQAERVFHQGFTGEEASGLELEMRRRDGK
                     PLWVSLWMRPLRGVDGQVQALHSIWVDITDRVLAEADRARLSEQNIYLQQEIKSVHNF
                     EEIVGRSPALAAVLDKVCRVASTDATVLIYGETGTGKELIARAIHSASQRRDKPLIKL
                     NCAALPGGLIESELFGHEKGAFTGAIARHTGRFELADGGTIFLDEIGEIPSETQAKLL
                     RVLQEHEFDRVGGTSTRKVDVRVIAATNSDLKKAVSDKAFREDLYYRLSVFPILVPPL
                     RERTEDIPLLAQYLLDKFATRVARRFDGIDSATLRRLVAYPWPGNVRELQNVLERAVI
                     LSAGPALEVEPDVLGYTDDAPAGRASAVLEVVDRDHIRAILQETKWVIDGPRGAAVIL
                     GLHPNTLRSRLKKLGLSRPGHEP"
     gene            2336..3364
                     /locus_tag="VHS97_10370"
     CDS             2336..3364
                     /locus_tag="VHS97_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3448648.1"
                     /translation="MRAMLLQATGPIRSNSAPLVAAEWPDPAPAAGEILIKVSACGVC
                     HTELDEIEGRMPPPSLPVVPGHQVVGRVEAMGEAADMFRIGDRVGVAWIFSACGHCEF
                     CRRGEENLCEQFQATGRDRNGGYAERISVSQSFAHAIPGVFTDSEAAPLLCAGAIGFR
                     SLRLTRLVDGQNLGLTGFGASGHLVLKLVRHQFPASRVYVFARSEAERDFARQLGAAW
                     AGDTTMNAPEKLHAIIDTTPAWTPVVEALKNLERGGRLVINAIRKETVDKQALLGLDY
                     PRDLWLEKEIKSVANVTRFDVREFLRLAAEIPIRPEVQEYPLEEANRALIELKGKNIR
                     GAKVLRVS"
     gene            complement(3450..4577)
                     /locus_tag="VHS97_10375"
     CDS             complement(3450..4577)
                     /locus_tag="VHS97_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX3448649.1"
                     /translation="MQPIKRRDFLKTVMIAGVTVNGAQGVTADPQPVKKDSGKWRGVN
                     LGGWLALEKWITPSVYTGLRAPDEYSLCEELGKAKAAAHLKKHRESWITADDFKWMAA
                     HGLNAVRLPVNYAVAEENPPFITAMDSVDWAFRTANAHGIGVLLDLHGVPGSQNGFDH
                     SGRQGKLGWHTSKNNIDHSLRIIADLAARVKGYDNLIGFELLNEPRWDVPLDVLKGFY
                     MDAYHRVRDQIGKDKAAVVMHDGFRQLEWGDFMRESDYANVILDTHTYQCFTDDDRKR
                     NLHQQIVVALVDRKRQLDAMQRQFPSIVGEWSCGLPPESLEGRTGFALDVAMRAYADA
                     QLINFDATRGWFFWTYKTQEGGAWSFRDCVQRGWMPDKYGL"
     gene            4791..4913
                     /locus_tag="VHS97_10380"
     CDS             4791..4913
                     /locus_tag="VHS97_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448650.1"
                     /translation="MARLREIAPWLNGATDQTSKLVAMLEKLLVKELHIGIFRC"
     gene            5030..5572
                     /locus_tag="VHS97_10385"
     CDS             5030..5572
                     /locus_tag="VHS97_10385"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX3448651.1"
                     /translation="MSETYLALLRGVNVGGKNKLPMKDLVDICLEAGCSNVKTFIQSG
                     NVIFSADRQIRAQLPGQIASLITGRFGFRTPVILRTLKQLTAVVANNPFLEQGGSEDA
                     LHVLFLADVPNADVVAGLDPERSPGDAYLVRGQEIFLKLPNGVADSKLTNIYFDKKLG
                     TISTGRNWRTVTKLLELMKG"
     gene            5653..>6284
                     /locus_tag="VHS97_10390"
     CDS             5653..>6284
                     /locus_tag="VHS97_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX3448652.1"
                     /translation="MATYQFPDGFVWGVATASAQIEGAARLDGKGESIWDRFATLPGK
                     IKNGDTPLVACDHYHLYEADFDLLKRLGVSNYRLSVAWPRVIPTGDGTINSLGLDFYS
                     RLIDALLERQITPWVTLFHWDLPQTLEDRGGWVARSTLDAFGCYADEVVRRLGDRVKH
                     WFTVNEIPCFIGNGYGNGYFAPGRRVDARDVNQAYHHALLAHGRAVEAVRS"
BASE COUNT         1218 a         1913 c         1904 g         1249 t
ORIGIN      
        1 ggctcgtggc ctgggcgtga gaggccgagc ttcttcagtc ggctgcgaag cgtattgggg
       61 tgaaggccga gaatgacggc ggcgccgcgg ggcccgtcga taacccactt tgtctcttgc
      121 aagatcgcgc gtatgtggtc gcgatcaacg acttccagca cggcggaggc acggccagcg
      181 ggcgcgtcgt cggtgtagcc aaggacatcg ggttccactt ccaatgcagg gcccgccgac
      241 aggatgactg cgcgctcgag aacgttttgc agctcgcgga catttcccgg ccacggatac
      301 gcgaccagtc ggcgaagggt ggcggaatct atgccgtcaa accgacgagc gactcgggtc
      361 gcaaacttgt cgagaagata ctgggccagg agaggaatgt cctcggtccg ctcgcggagc
      421 gggggaacga gaatcggaaa aacgctgagg cggtagtaca gatcctcacg aaaggccttg
      481 tcgctgacgg ctttcttgag gtcgctgttg gtggcggcga tgacccgcac gtcgactttc
      541 ctcgtagacg tgccaccaac ccggtcgaac tcgtgctcct ggagcacgcg caagagtttg
      601 gcctgggtct cggacgggat ctcaccgatc tcgtcgagga agatcgtccc gccgtcggcc
      661 agctcaaacc tgccggtgtg ccgcgcaatc gccccggtga aagccccctt ttcgtggccg
      721 aacagctcgc tctcgatgag ccccccgggc agggctgcgc agttgagctt aatcagcggc
      781 ttatcccgcc gctggctggc ggagtggatc gcacgggcga tcaattcctt gccggtcccc
      841 gtctcgccgt agatcaagac cgtggcgtcc gtagatgcga cccgacaaac cttgtcgagc
      901 accgcggcca gggccgggct gcgacccacg atttcctcga agttatgaac ggacttgatc
      961 tcttgctgga gatagatgtt ctgctcgctc aggcgagcac ggtcggcctc ggcgagcacc
     1021 cggtccgtta tatccaccca gatcgaatgc aacgcctgca cctggccgtc gaccccacgc
     1081 aaagggcgca tccacaggct gacccagagc ggcttgccat cgcgccggcg catttccagt
     1141 tcgagccccg aagcctcttc gcccgtgaac ccttgatgaa atacgcgttc ggcctgcacc
     1201 acgccggcgg gcgtcgcggc gaagtgcgcc aggacgttcg agccgaccag ctcggcggcc
     1261 ggatggtcca aaagctgcgc cgctcggcgg ttcacgctca gcagttggaa ctttctgtcg
     1321 aaagaaacat acgcattcgg tgcctcctcg tagagatcgc gataccggcg ctcactctcg
     1381 atcagttgtt tctcgaccct cacgcgttcc agctcggcgg ccgcgcgggt cgcaaaaatc
     1441 cggaagatga acagcagccg cggctctggc gccatcggcc gcccgtcaaa gaccgccaga
     1501 tggccaagca cggttccttc accgtccagc aaaggcaccc cgagatagct ctcgattccc
     1561 atggccgcaa gatcccggtc gtccggaaat ctgtccttga cccccaaggg gtggtggcac
     1621 aaaccgccac gcacgacatc ctcgcaaggc gtgccggcca ggtcatactc gacgttttcg
     1681 cggagtttac ccttgctcca gtaggcgagc gttcgcaccc gctcgttact gccggcaaac
     1741 tcggcgacga acgcgtaact aacatcgatc gctcgggcaa ggtgccttac cagcgactcg
     1801 aagaatactt cacccgtgtt tcgggcagtc ccctcgacaa tcgctctcag ggccgcgatc
     1861 tcgtcatcgc ccgatagcgt cgctcggtcc ggctcgatcg cgttcttagc tcgataatgg
     1921 ccggtcgatt gacgtcccat tcagtcgttc tccacttggc gctctaggac aagccacatg
     1981 acgtcgtgac gcaaatcacc cacgctcgac caacccagaa gaagcttggc gatcacggcg
     2041 cgcttcccat gtggcggccg agactgagac ggccctctca ccaaagacgc ggcgacaccc
     2101 cttattgttc gatctcgata caagttttca atgactgcgt gcaggggcgt gcgtgatcca
     2161 aagccgttgg aaaacaagag ttcatgcgcc ggatgatcac ctggacgtgc ttaagtcgtc
     2221 ggttgtcgga ggtaccgcct cgtgtgggat ttattacgat agcaaggtgc tgatggaatc
     2281 gatcaaagca gtgagcaact ggaagccggt ctgattcacg ggaggtgagg caacaatgcg
     2341 tgcgatgtta ttgcaagcca ccggcccgat ccgcagcaac tctgcgccgc tggtggcggc
     2401 agagtggccc gaccccgcgc cggcggctgg agagattttg atcaaagtga gcgcctgcgg
     2461 cgtctgtcac accgagctcg acgagatcga ggggcggatg cctcccccga gcctgccggt
     2521 cgttccgggc catcaggtcg tcgggcgcgt ggaagccatg ggagaagcgg ccgacatgtt
     2581 ccggatcggc gaccgggtcg gagtcgcctg gattttttcg gcgtgtgggc actgcgaatt
     2641 ctgccggcgg ggggaggaga atctctgcga gcagtttcaa gcgaccggcc gcgatcgcaa
     2701 tggcggctat gccgagcgga tctcagtatc gcagtcgttt gcccacgcga tcccgggcgt
     2761 cttcaccgac tccgaggccg cgccccttct atgtgcggga gccatcggct tccggtcgct
     2821 gcgtctcacc aggctggtag acggccagaa cctcgggctt acaggctttg gtgcgtcggg
     2881 gcaccttgtc ttgaagctgg tgcggcatca gttcccggcc tcgcgcgtgt atgtgttcgc
     2941 gcggtccgag gccgagcggg atttcgcgcg gcaactgggc gcggcctggg caggcgacac
     3001 cacgatgaac gcccccgaaa aactccacgc gatcatcgac acaaccccag catggactcc
     3061 ggttgtcgag gcgctcaaaa acctcgagcg cggcggccgg ctcgtaatca acgccatccg
     3121 caaggagact gtggacaagc aagccctgct tgggctcgat taccctcggg acctgtggct
     3181 cgaaaaggag atcaagagcg tggccaacgt cactcgcttc gacgtccgcg aattcctgcg
     3241 ccttgccgcc gagattccca tcaggccgga ggtccaggaa taccccctcg aagaggcgaa
     3301 ccgtgcgttg atcgagctca agggtaagaa cattcgggga gcgaaggttc tgcgcgtatc
     3361 gtgaacgata atcgcgacgc ggattgacgg cgccggcaat ggtcggacgt tgcacagact
     3421 ccggacagtg atatccggca gtcgcctcct tacaagccat acttgtctgg catccagccg
     3481 cgctgtacgc aatcgcggaa gctccacgcg cctccttctt gtgtcttgta ggtccagaaa
     3541 aaccagcctc gcgtggcatc aaaattgatt aactgggcgt cggcataagc tcgcatcgca
     3601 acgtcgagcg caaagccggt ccgtccctcc agcgattcgg gtggaagccc acaggaccac
     3661 tcccccacga tggagggaaa ctggcgctgc atcgcatcga gctgcctctt ccggtccaca
     3721 agcgcgacca cgatttgctg atgaagattg cgcttgcggt cgtcgtccgt gaaacactga
     3781 tacgtgtgcg tatcgaggat cacattggca tagtccgact cgcgcatgaa gtcgccccat
     3841 tcgagctggc gaaatccgtc gtgcatgacg accgcggctt tgtccttgcc gatctggtcg
     3901 cgcacccggt gataggcgtc catgtaaaat cccttgagaa catcaagcgg cacatcccac
     3961 cgcggttcgt tcaatagctc gaagccgatc aagttgtcgt agcctttcac tcgcgccgct
     4021 aggtccgcga tgatccgcaa cgagtgatca atattattct tgctggtatg ccagcctagc
     4081 ttgccctggc ggccgctgtg gtcaaagccg ttctggctgc cgggcacgcc gtgcaggtcg
     4141 agcaagacac cgattccatg ggcattcgcc gtccgaaacg cccagtcaac agaatccatc
     4201 gcagtgatga atggggggtt ttcctcggcg actgcatagt tgaccgggag ccggacggca
     4261 ttgagcccgt gcgctgccat ccacttgaaa tcatcagcag tgatccacga ctcgcgatgt
     4321 ttcttgaggt gtgcggcggc cttcgctttg ccgagttcct cgcatagtga gtactcgtct
     4381 ggagcgcgta gtcctgtata aacactcggc gtgatccatt tctcgagtgc caaccatccc
     4441 cccaggttga ccccgcgcca tttgccgctg tccttcttga ccggctgcgg atcggcagtc
     4501 acaccctggg cgccgttcac ggttactccg gcgatcatga cggtcttcaa gaaatcgcgc
     4561 cgcttgatcg gttgcatttt acggcccctc gtagtttgtc tgaccctggc gttgacgata
     4621 atggcgcgtc acacgcaagt ccagtggctc cacaattgac ttcggtagct ggaaggacta
     4681 ttcttacgct cgtgtggcga gagcatcgcc gcacatttcg ccctagcggg cttttcatgg
     4741 ggccggtagg cccagaggag aattcgataa cagaggagct gaaggagcgt atggccaggc
     4801 tgcgcgaaat cgcaccctgg ctgaacggtg cgaccgacca gacatcgaag ctggtcgcta
     4861 tgttggaaaa gctcttggtg aaagagctgc acatcggcat cttccgatgc tgagtaaccc
     4921 ggtcggccga ccgagctcag cggtgactta gcgggactcg gtcggcatcc gggcacaacc
     4981 aacgtgtggc aattcgccgc aattcgaagc acgcgaaagg ctgttgcgga tgtcagagac
     5041 ctacctggcc ctcctgcgag gagtcaacgt cggcggcaag aacaaactgc cgatgaaaga
     5101 ccttgtcgat atctgccttg aggctggatg ttctaacgtt aaaacgttta tccagagcgg
     5161 gaacgtgata ttcagcgccg accgtcaaat ccgggcgcaa cttcccggtc agatcgcttc
     5221 gctgattacc ggtcgcttcg gatttcgaac gcccgtgata ttgcgcacac tcaaacaact
     5281 cacggccgtt gtcgctaata accctttcct ggagcagggg gggtctgaag atgcgctcca
     5341 tgttttgttc ctggcggatg tcccgaacgc cgacgttgtc gccggtcttg atcccgagcg
     5401 ttctcccggg gacgcgtacc tcgtgcgcgg ccaggagatt tttctcaagc ttcccaacgg
     5461 tgtggccgac tccaagctca ccaatatcta ctttgacaag aaactcggca caatcagcac
     5521 agggcggaac tggcgcacag ttacgaagct cctcgagctg atgaagggat aattcgcctg
     5581 atttcggaga gtggcccggg taagagcccc gaagccgttg atcactaggc gatctcgcgg
     5641 agagaaactc cgatggcgac gtaccaattc ccggacggct ttgtgtgggg tgtggcgacg
     5701 gcctcggcgc agatcgaggg agcggcgcgg ctcgatggca agggcgaatc gatctgggat
     5761 cggttcgcca cgctgcccgg taagatcaag aatggcgata cgcctctagt cgcatgcgac
     5821 cattatcatc tctatgaagc cgatttcgac ctgctgaaaa ggctgggggt ctcgaattac
     5881 cggctgtcgg tcgcctggcc gcgcgtgatc cccacggggg atggcacgat taactcgctc
     5941 gggctggact tttactctcg attgatcgat gccctgctgg agcgtcagat cacgccttgg
     6001 gtcacgctgt tccactggga tttgccccag acacttgaag accgtggggg ttgggtggcg
     6061 aggtctactc tcgacgcctt cggctgctat gcagatgaag ttgtcaggcg gttgggcgat
     6121 cgtgtcaagc actggttcac cgtcaacgag attccctgct tcatcggtaa tggatatggc
     6181 aacggctact tcgcgcccgg gcggcgcgtc gacgctcgcg acgtaaacca ggcttatcat
     6241 cacgccttgc tcgcacacgg gcgggcggtc gaggccgtcc ggtc
//
LOCUS       DATAQL010000594         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4333632, whole genome shotgun sequence.
ACCESSION   DATAQL010000594 DATAQL010000000
VERSION     DATAQL010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4333632"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VHS97_10395"
     CDS             complement(<1..683)
                     /locus_tag="VHS97_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448653.1"
                     /translation="MTIKWARDSRWIPGPVDLVFVLVLASLLIGGRHALLNDPGTPWH
                     LRLGREILANGAVPRYDTLTFTHDHASWVDQSWAFDVLLALLVDSWGWSGAIGLASLG
                     LATLYAAMARGLIRDGNSPVVAMVVSLFATAIGAIHFLIRPHLFTFAFVYLTLRACQK
                     QHQRGGWAVATIPFYTAILANLHGGFVALPVIVATAAFGHAISGAWDNARRRNVAKFG
                     LTILACGIAG"
     gene            956..2035
                     /locus_tag="VHS97_10400"
     CDS             956..2035
                     /locus_tag="VHS97_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX3448654.1"
                     /translation="MKAPRPGVNRRDLLRFRRNKEVRAALKRSSPGDPGRYDLIRVHR
                     PAMGSYFEVRLPASIPGAVDLACRGLDVIELLEAQMTVYRDDSEVSRLNATAHRGPVA
                     VERGLFELLEMAVGISRETGGAYDVAAGALSEAWGFVKGPKQVPDEIALANARARTGW
                     HHLQLDSERHTVAFDREGVRINLGSIGKGFAIDRAVDVLRAHWWPVSALIHGGQSSLY
                     ALGSPPGQFGDRWEIALRNPFEPELPLGTLRLRNRGLGTSGAAFQQFVVDGRVYGHII
                     DPRSGEPAIGPASVTVLAPTAALADALSTAFYLLGPEAAAPYVAQHPEVGVVFVDKRP
                     EQPSPCLLTFGLDDHDFVVAGNTVH"
BASE COUNT          414 a          659 c          698 g          433 t
ORIGIN      
        1 cccgcgatgc cgcacgccag gatcgtgagc ccaaacttgg ccacgtttcg gcgtcgcgca
       61 ttatcccagg ctcccgagat tgcgtggccg aatgcggcag tggccacgat caccggcagt
      121 gccacgaacc cgccgtgcag attcgccaga atcgcagtgt aaaaggggat ggtcgcgacc
      181 gcccagccac cccgctggtg ttgcttttgg caggcccgca gcgtcaggta gacgaacgca
      241 aacgtgaaaa ggtgcggccg gatcaggaaa tggatcgcgc cgatcgcggt agcgaacagg
      301 gaaactacca tggcgacgac cggcgaattg ccatcgcgaa tcaatccgcg ggccatggca
      361 gcataaagtg tggcgagacc gagagatgcc agcccgatcg ccccggacca gccccatgag
      421 tcgaccaaga gcgcgagcag gacgtcgaac gcccatgact ggtcgaccca cgaagcgtga
      481 tcgtgggtga acgtcagggt gtcataccgg ggcacggctc cattggcgag gatctcgcgc
      541 cccaaccgca ggtgccaggg tgttccggga tcgttcaaga gcgcatgacg gccgccgatc
      601 aaaagcgaag ccagcacgag tacgaagacc aggtcgactg gtcctgggat ccaccggctg
      661 tcgcgcgccc atttaatggt catgtgcatg tcacgtcggt cttctattgg caatttcgtc
      721 taccaacagc ttaggttacg ttcgcgctgc gaatgctttt tttgagcggc agcagttgga
      781 tcttcgctct ggaagggagc aggcgtttcg agattggtct cttgaccact tttccttgac
      841 ctcttctcgt agtgatgccg tcgggtatac tgtgaaacga cgcatcgggc gccggccctg
      901 gtcaccggcc gtcggtgcga ctccccccct gccccagtaa agtgcgagtc gtcgcgtgaa
      961 agcccctcga cctggcgtga atcgccgcga tcttttgcgt ttccgccgca ataaagaggt
     1021 tcgagctgcg ctcaaacgca gttccccagg tgacccgggc cgttatgatc tgatccgagt
     1081 ccatcggccg gcgatgggtt cgtacttcga ggttcgcctg ccggcgtcta ttcccggcgc
     1141 ggtcgatctg gcgtgccgcg gtctggacgt gatcgaacta ctcgaagccc agatgacggt
     1201 ataccgcgac gattccgagg tcagccggct gaatgcgacg gcccaccgag gtcccgtggc
     1261 agtagagcgc gggctcttcg agcttctgga aatggcagtc ggcatcagcc gagagaccgg
     1321 cggtgcctac gacgtggccg cgggcgcgct ctccgaggcg tgggggtttg tgaaagggcc
     1381 caagcaagtg ccggatgaga ttgcactggc gaacgctcgg gctcggaccg gctggcacca
     1441 cctgcaactc gactctgagc gccacacggt cgcgttcgac cgcgaaggcg tgcgaatcaa
     1501 cctggggagc atagggaaag gtttcgccat cgaccgcgcc gtggacgtct tgcgagcgca
     1561 ttggtggccc gtgtcggcgt tgatacacgg cggccagtcg agtctctacg cccttgggtc
     1621 gccaccggga cagtttggcg accgctggga aatcgcatta cggaatccgt tcgagccgga
     1681 gttgccgtta ggcaccttgc ggctacgcaa tcgcgggctc ggaacctcgg gtgcggcgtt
     1741 tcaacaattc gtagttgatg gaagggtcta cgggcacatc atcgacccga gatcgggaga
     1801 gccggcaata ggacccgcga gcgtgaccgt tttggctccg accgcggcct tggcggatgc
     1861 cctgtcgacg gctttctatc tactcggtcc ggaagccgcc gccccttatg tggcgcagca
     1921 tcccgaagtc ggcgtggtct tcgtcgataa aagacctgaa caaccgtcgc cttgtctgct
     1981 tacattcgga ttggatgacc acgatttcgt cgtggccggg aacacggtac actaaaacgg
     2041 tggttcacca gattgcaccc ttgaggcgcc tgcgcgtatg tcccgctatt atcctgggtt
     2101 cctggctgca ttttttatcg tcctcttgcg aatcgcgatc ggctggcatt tcctctacga
     2161 ggggttcgag aaggtcgact cgatgagtta cggcaaagac ccgt
//
LOCUS       DATAQL010000595         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_60617, whole genome shotgun sequence.
ACCESSION   DATAQL010000595 DATAQL010000000
VERSION     DATAQL010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60617"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..932
                     /locus_tag="VHS97_10405"
     CDS             162..932
                     /locus_tag="VHS97_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3448655.1"
                     /translation="MKLYPAEATGSGVVSHDGVYRGGSLHGDVEVDAEHTLIMACCDP
                     AVGLLAAELARAADVRLIAFPRSSRTAMSLIGQGLVHVAGVHLARAEQAGGNAAIVMR
                     ELGAGYNLLRVARWEEGIAFAPGVRVPSVRAAVDSNLRWVGREDGSGARQCLDELFGD
                     HRPPRRLASDHRGVAEAVRSGWADAGVCLRLVSEEAGLEFLPVREETYDLCFPDRWEG
                     DHRIQALLHVVRSQSYRRALGELPGYDSAETGELHRVS"
     gene            1169..1645
                     /locus_tag="VHS97_10410"
     CDS             1169..1645
                     /locus_tag="VHS97_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3448656.1"
                     /translation="MKFLCLVYHEEEKLAALSQRQMDTLVGACIGWAEELEKSGHHIL
                     SAGLQSVRSAATVRVRNDKLSTTDGPFAETKEVLGGFTLIDAKDFDEALQIAAKFPAA
                     QIGSMEVRPVLEPGEAVADPLDQKIQASILQNASGFNPFAASKMVSLPRPDTTEEQ"
     gene            1642..2004
                     /locus_tag="VHS97_10415"
     CDS             1642..2004
                     /locus_tag="VHS97_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3448657.1"
                     /translation="MRYLCMVCYEQRTLDSLSKPEFDSLVAEALAYDDELRKGGHYLY
                     SGALQSVETATTLRIKSGKASITDGPFAETKEHLGGFIVIEARDLNEAIRLASKIPPA
                     RLGCIEVRPIQELEPRRV"
     gene            3109..>3395
                     /locus_tag="VHS97_10420"
     CDS             3109..>3395
                     /locus_tag="VHS97_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448658.1"
                     /translation="MSIGEDQVARVPPLRQGRGLRISILGMLALVAAVAAAMVVYRRV
                     SEKVDERPSLIAKYDKTAPVADGVIGPKEYGPVVFVQWTEGNTLTAFRREL"
BASE COUNT          651 a         1012 c         1060 g          672 t
ORIGIN      
        1 ttatcccgtc ggccgcagcg gccctggcct tggcagcggc gctcgaatgc cgggtcgagg
       61 acttgttccg gctacgcggt gccgagtctc gcgaatcttc ttgggcttgg tcccctcttc
      121 gctaaccctg tcgatactgg aaggccaagg tgggcgggag catgaagctc tatcccgcgg
      181 aagcgaccgg gtcgggggtt gtctctcatg acggtgttta ccggggtggt tcgcttcatg
      241 gtgatgtgga ggtcgatgcc gagcatactc tgatcatggc atgttgcgac cccgcggtgg
      301 gattgctcgc tgccgagctg gcccgcgccg cggacgttcg tctcatcgcc ttcccgcgtt
      361 cgagccggac ggcgatgtca ttgatcggcc aaggtttggt gcatgtcgct ggcgttcatt
      421 tggcgcgggc cgagcaggct ggaggtaacg cggcgattgt catgcgtgaa ctcggcgctg
      481 ggtacaacct gctgcgcgtc gctcgttggg aggaagggat cgcctttgca cctggtgtcc
      541 gcgtcccctc cgtccgcgcg gcggtagatt ccaatctgcg gtgggtcggc agggaggatg
      601 ggtccggggc gcggcaatgc ctcgatgaac tcttcggcga ccatcggccc ccgcggcggc
      661 tggcctccga ccaccgcggc gtcgccgagg ccgtccgaag cggatgggcc gacgcggggg
      721 tctgcctgcg cctcgtgagc gaggaagccg gcttggagtt tctccccgtc cgcgaagaga
      781 cctatgacct ctgctttcca gatcgttggg aaggagacca tcggatccag gcgctgctcc
      841 acgtcgtgcg ctcgcagtcg tatcgcaggg ccttgggcga gctaccgggc tatgacagtg
      901 cggagactgg cgagctgcat cgtgtcagtt aattgaccgc cacggttccc gcccggctgc
      961 cacaccggac agtgaatgcc aaagaccatg atggcgggga ataaacattc gaaggcagcc
     1021 gctggcgcga tgacaagtac ccggcccatg agagcgcagc aagcggcttt gccgacgggt
     1081 cgaaaaaaac cggctattgt cgatctggcg tttcgccgat cgactagagg gtgttgcaac
     1141 cgagtcgaaa ctccgatcgg aggtcgccat gaaatttctt tgcctggtct accacgaaga
     1201 agagaagctt gccgctttgt cccaacgcca gatggatacg ctggttgggg cgtgcattgg
     1261 ctgggccgag gaactggaga agagcggcca ccacatttta tcggcaggcc tccaatcggt
     1321 tcggtccgcc gcgaccgtgc gcgtccgcaa cgacaagctg tcgaccaccg acggtccgtt
     1381 cgccgagacg aaggaggtcc tgggtgggtt cactctcatc gatgccaagg acttcgacga
     1441 ggctctgcag atcgccgcga aattcccggc ggcacagatc ggaagcatgg aggtgcggcc
     1501 ggtgctggaa cccggggaag cggtggccga tccactcgac caaaagatcc aggcgtccat
     1561 ccttcagaac gcatcgggat tcaatccgtt cgcggcctcg aaaatggttt ctctcccccg
     1621 acccgatacg acggaggaac aatgagatac ctctgcatgg tctgctacga gcagaggacg
     1681 ctcgacagtc tttccaagcc cgaattcgac tcgctcgtgg ccgaagcgct ggcgtacgac
     1741 gacgagctcc gcaagggggg acactacctc tactcgggcg cattgcaatc ggtagagacg
     1801 gcaacgaccc ttcgcattaa gtcaggtaag gcgtcgatca ccgacggccc attcgccgag
     1861 acgaaagaac atctcggcgg ctttattgtc atcgaagcgc gcgacctcaa cgaggcgatc
     1921 cggctcgcct ccaagatccc gccggcacgc ctcgggtgca tcgaggtccg tccgatccag
     1981 gaacttgaac cgcgccgggt ctaaaccgcg cttcactact agtacttgaa tctcggctca
     2041 taggagaaac aatcacgacg atgaccgcca cgtgacgctc gacgaatccg tcgcaccgat
     2101 cgaatggaaa tacaaagaca atgcgacgct cgagtagggc ggcgacagct ggttccagaa
     2161 cgccgaaacg accggagtgg gcgttttcgt ccgcgcagac aacgaggtct ttcacactta
     2221 gggttttcag agtggtactc tggaacaatg ccaacggtcc ttcagccctg accatatagg
     2281 tttttcgtaa cccttcagcc gttttcagta atcggcccct tggggcgaca gcggccgact
     2341 gttgtccaga tgatttcaag ttttccgcgc ctagtctagc cgccgcttta ggcggggatc
     2401 tcacactgcg tttccttcgt ttccttcgtt tccttctgtt ccaggcttgt tagcacgggg
     2461 caggacgagc cttgatattc cgtcaacgag ctcgacctca tggaagttga ataagagtcc
     2521 cagaggcaca ttcaggagtt tgatactctc caacagtcct ggccccatga tccggtgcac
     2581 tagggtggcg gcgccaataa cctcaccgga gagcctgtcg gcgctgacga atagcaagtg
     2641 catagtgttg ttctcatctc cgaacagaag gaaacgaagg aaacgaagaa aacaattaac
     2701 atcgagatcg gaagttggag cacatgtgat tttcccacga ttcgtgaagt cggaaccaca
     2761 cgggcgcgcc ccttactggc cggactcggc tggcaatgct ttgcatgtcc tttcggctca
     2821 tccctgtcaa ggtcacgagt acctctcggt gttggagcgg ctcccggctg aaatccgtca
     2881 gggagtcaga gccatcgtcc tgcgccgcac accaaagtat tggcgctcgt catcgcctcc
     2941 ccagcgtgct ccatcttccc ctaggaccgg tcgaaccgcc ctcgacggaa aatgccaacg
     3001 gtgtcaattg tgactcacgc gcctttcttt tgacatctgt ccgaggtaag atgccggcgt
     3061 tcggtcgcgt gaggccggcg cgtcgaccgg cgtccgggag gcaagcccat gtcgatcggt
     3121 gaggatcagg tcgcccgcgt accgccgctg cggcagggcc gggggttgcg gatcagcatt
     3181 ctggggatgc tcgcgctcgt cgcggcggtg gcagccgcga tggtggtcta ccggagagtc
     3241 tccgagaagg tcgacgagcg gccgagcctg attgcgaagt acgacaagac cgcaccggtg
     3301 gccgacggtg tcatcgggcc gaaggagtac ggccctgtcg tgtttgtcca atggaccgag
     3361 gggaacaccc tcaccgcctt ccggcgcgaa ctgca
//
LOCUS       DATAQL010000596         9467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2198319, whole genome shotgun sequence.
ACCESSION   DATAQL010000596 DATAQL010000000
VERSION     DATAQL010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198319"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VHS97_10425"
     CDS             complement(<1..854)
                     /locus_tag="VHS97_10425"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448659.1"
                     /translation="MAEQPLEEKFTTALEALVEHVKEDRSVLAAILCGSLSHDKVWAK
                     SDIDLLLVTIDDKKVAEGYLALYADGLNVHAILVPRAQFRKTMEGAIRNSFMHSLVAK
                     GRLLYTHDETITDLCARLEGIGERDTQVQLLNAATRALGPIYKAHKFLITRGDLDYTA
                     LWILYAATPLAQIEVIGQRLLADREVILQAMKLNPAFFKTVFADLLNGKKTRKNVEAA
                     LESVDRYLAERAAKLFAPVIDHLREVGEARSCTEIEGHFQRNLNVSGVYAACEYLADQ
                     GLIGKASTP"
     gene            complement(1034..3301)
                     /locus_tag="VHS97_10430"
     CDS             complement(1034..3301)
                     /locus_tag="VHS97_10430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3448660.1"
                     /translation="MIADSIWTTAETGLFGLGINGLLLVGSYWIARHGFRQPRGLNRA
                     LATAVVFWTACTLGSEILGISGALAQGPLLGWGILVAGIGYGLWWFCPSIDRDPPHDA
                     TKEPFSWDELVSVALMSWVTLMLGVRSLLLGVKVVSDGPIYHLYFAVRWWKAGRLLLV
                     AAPFGESAATYFPANGDLWFAWLMTTWGGDRLARIGQVPFLFLASLAAFGCARVLGAT
                     RAASLVATCWFASSMPLFIFSFEPNVDTIFVAGYLLAAYFFLQAGRAEGQLAAYCLSA
                     LAAGLAMATKPTAVVFMPPLVVVAIVENVLQSIPIRTKIVRAGVIALVPLVSGGFWFV
                     RNWVLTGNPLYPLEVRLLGRTLLPGWYGPDAMRTSPYYLPITDWRALGDILLAVLDPR
                     LAPIWIVSLFMGWAIKGPRTAEKNGRIALFAALAVLNVALYWVCIPYRTQQRFMLQAL
                     GLAVVPLAVLLDRGRWLRLTAVVLLGLHLLTPQGWPLVGPGGAIPWDLTPLIPNAVGD
                     PVMMSSRITRAFESDYAKRSVMALACAVGSGLAALVMVGAWNRVAVSAPRFGRALTTA
                     VVWSVLFLGLGYLDVWRDMSDPRLDFYPVFPDFFAGWLNLEYRSGLRGARVAYAGTNI
                     PYYLMARGLRNEVRYVNTDRHRNWLLHDYHRAASTLGHGHWPNSRPGWDRMRPDFQAW
                     LDNLDAEGIELLVVTRVNPGEGAHNVADVENFPIERGWADSHPERFEILYGRRENDPW
                     FRLYRVRRSKATMGN"
     gene            3636..4247
                     /locus_tag="VHS97_10435"
     CDS             3636..4247
                     /locus_tag="VHS97_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3448661.1"
                     /translation="MIGAKLGISDYLARVCEEIQRLDPAQCEKVCELVESAYDEGRFV
                     FIIGNGGSGANASHLCEDLAKCTLHDFENQKRLKVLSLTDNTAGIMAWANDEGYERIF
                     IEQLKNLASPGDLLLAISGSGNSPNILKAVSWANDHGLTTVGFTGFSGGKLKSLAHHN
                     LHAGIDDMGIVESLHQVVFHWIIDDLYRRFSLTAAVSNGVKTG"
     gene            4252..5244
                     /locus_tag="VHS97_10440"
     CDS             4252..5244
                     /locus_tag="VHS97_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3448662.1"
                     /translation="MSEFWALGIEIGGTKLQLGIGQGQGSLLALDRLRVDPSRGAAGI
                     LEQIKDALPRLLAAAKLDRHQIQAAGIGFGGPVDVQNGRTQKSFQIVGWDDFPLAGWL
                     GEHLEISRVVLENDADTAGLAEARFGAGVGHSPLLYMTVGSGIGGALIVNRQIYRGSG
                     HGAAELGHLLVPETTPSGIRLRELEQVASGWAIAAAARELARRKLHDGRGEWIVLSQA
                     QGRPDDISTDLVANAARAGDPDALTILDHASTAISFALTQTVALLAPQRIVIGGGVSL
                     IGDKLWFDPIRCLLDRDVFGPFRGRFDIVPAVLGEQVVVHGALALARDSIASAG"
     gene            6122..6364
                     /locus_tag="VHS97_10445"
     CDS             6122..6364
                     /locus_tag="VHS97_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448663.1"
                     /translation="MTAKLAETQLWQQNMASLIRSGLFSQATTGEMNGLYTVVGVYVD
                     ETFSAPLAKYSDLRRATDAANLVNQLAKVRRSIESN"
     gene            6519..8501
                     /locus_tag="VHS97_10450"
     CDS             6519..8501
                     /locus_tag="VHS97_10450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3448664.1"
                     /translation="MEVTSQRSRLRLVLFAVALVGHRPAPPVDEWTAPRQTLQAIGRR
                     LSQSNSEHALTVIASREDLILERLKGQERAALAHGYLRFRVDTPVVVDVAVPAETIPF
                     WIGDLGFRPTDLTLENSDCSWTLYRKTFGAGVIGLGINGLDRRPLAHYAVFVRPVQTT
                     AADPRRPVVQLDSRQTSLWKITLARAGVSAANDTYKPFESLPAELIDAILIQPAHGAR
                     HSALLATGRVWKTHVVSSSRPDQVVVGFGSDPGRELVWTWRTSAKITSSAIRIARAPE
                     SNRNNPSADDSKAFPGSVRVVNGDSTPVEATNLLNDAVTQRHHVVVDGLQPDTVYQYS
                     LGDGTPGGWGSWETVKTAPDSSRGARFLYLGDAQTGLEGWGRLLKAASHQNPDIDCVL
                     LAGDLVDRGNERTNWDHFFLRGAGVFDRVPLMPCVGNHEYLDMGPRLYRAFFDLPRNG
                     PTGIDTDLVYQFECGNACMVVLDSTLAVCDPAAARRQANWLDATLTRSKASWKFVMFH
                     HPVYPSHPWRDSPAMREHWVPIFDKHHVDLVLQGHDHAYQRTYPLRGHRRVDTPGDGT
                     IYVIAVSGDKFVEQAARDYVEVGRSGISTYQTIEIDSPSNRLTYRARTEDGTIVDELQ
                     IEKALQPRRHSEPPLSGPTPTIAPTLTRAALAPGLP"
     gene            complement(8641..>9467)
                     /locus_tag="VHS97_10455"
     CDS             complement(8641..>9467)
                     /locus_tag="VHS97_10455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX3448665.1"
                     /translation="LGINPDELTRVDVHVCDQDRILIARRLSEEWPRRSTRRSESYAT
                     AAVLHRAGVGDRRETKLSTTHRGTGVLPESGHGLEGRATSIDSIAADADYLHADQFTS
                     RPPLLAVHLGAGNPAKRWPIGSWQALIDRFIESGWRIVVVGGIEDAHLSRVLEPHDRL
                     RDWTGSLTVTQTTALLERADLFIGSDSGPAHLAATAGTLSVILFSGTNQPGQWRPWSR
                     HSLVLRHRVPCQPCHQKVCPLANHPCMSGLSPDRVYVGAMRLWSRIHRAESPHSPI"
BASE COUNT         1986 a         2886 c         2749 g         1846 t
ORIGIN      
        1 ggcgtggagg ccttgccgat caacccctgg tcggccaaat actcgcacgc ggcatagacg
       61 ccgctcacat tcaggttccg ctggaaatgg ccctcgatct cggtgcatga ccgcgcttcg
      121 ccgacctcgc ggaggtggtc gatcacaggc gcgaacagtt tcgccgcacg ctcagccagg
      181 tagcggtcga ccgactcgag cgccgcctcg acattcttcc gcgtcttttt gccgttcaag
      241 aggtcggcga agaccgtttt gaagaacgcc gggttcagct tcatcgcttg aagaattacc
      301 tcccggtcgg caagcaatct ctggccgatg acctcgattt gcgccagcgg cgtcgcggcg
      361 tagagaatcc acagcgctgt gtagtccagg tcgccgcgcg tgataagaaa cttatgggcc
      421 ttgtagatcg gacccagagc gcgcgtcgcc gcgttcaaga gctgtacctg cgtgtcgcgc
      481 tcaccgatcc cctcgagccg ggcgcagagg tccgtgatcg tctcgtcatg ggtgtagagc
      541 agccgcccct tggccacgag cgagtgcatg aacgagttgc gaatcgcgcc ttccatcgtc
      601 ttgcggaact gtgcccgcgg caccaggatg gcgtgcacgt ttaacccgtc cgcgtagaga
      661 gccaggtatc cctccgccac cttcttgtcg tcgatggtca caagcagcag atcgatgtct
      721 gacttggccc acactttgtc gtgcgagagg ctaccacaaa ggatcgccgc cagcacggag
      781 cggtcctcct tcacgtgctc gactagcgcc tcgagcgccg tcgtgaactt ttcttcgagc
      841 ggttgctccg ccatgctcca ccaccgcggc cgctgatcgt atttccgcca cccgacgatt
      901 ccgcgcgcac tacgtcgcga tattattcta tctgacgagg atcggctgtc atactcgtgc
      961 tcatcacaca ccgccctact gtcgatccat agataagacc tattagcaat tcggttcgac
     1021 cgccggcctg aactcagttc cccatagttg ccttcgaccg ccgcacacga taaagacgaa
     1081 accacgggtc gttttctcgt cgtccataga gtatctcgaa tcgttcggga tgagagtctg
     1141 cccatccgcg ctcgatcgga aaattctcga cgtcggcgac gttatgcgcc ccctcgccgg
     1201 ggttcactcg agtaaccaca agtagttcga tcccttcggc atcgaggttg tcgagccatg
     1261 cctggaagtc gggtcgcatt cgatcccatc ccggccgaga attcggccag tgtccgtgcc
     1321 ctagagtcga ggccgcgcgg tggtaatcat ggagaagcca attgcggtga cggtcggtgt
     1381 tcacatagcg gacttcgtta cgcaaacctc tggccatcag gtagtagggg atgttggtgc
     1441 ccgcgtaggc cacacgcgcg ccacgcaagc ccgatcggta ttcgagattg agccagccgg
     1501 cgaagaagtc gggaaatact ggataaaaat ccagccgcgg atcgctcata tcacgccaaa
     1561 cgtcgagata tcctagccct agaaagagca cggaccacac aacggccgtg gtcagtgcgc
     1621 gaccgaaccg cggcgccgag accgcgacgc ggttccaggc gccgaccatc acgagcgccg
     1681 ccaaaccact accgaccgcg cacgccaagg ccatgacact ccttttcgca taatcggact
     1741 cgaacgctct ggtaatcctc gacgacatca tcaccggatc acccacggca ttaggtatga
     1801 gcggcgtgag atcccacgga atggcgccac caggaccgac aagcggccag ccttgagggg
     1861 tgagcagatg caatcccagc aggacgacgg ccgtgagacg cagccatcga ccacgatcga
     1921 gcagcaccgc cagcgggacg acggcaagcc cgagcgcctg gagcataaaa cgttgctgag
     1981 tgcggtaagg aatacaaacc cagtagagtg cgacattcag aacggcgaga gccgcaaaca
     2041 gcgcgattcg gccgtttttc tctgcggtcc ttggtccctt gatcgcccag cccatgaata
     2101 gtgacacaat ccagatcggc gcgagccgcg ggtcgagcac ggccagcaag atgtcaccca
     2161 gcgctctcca gtccgtgatc ggcagatagt aagggctggt acgcatcgcg tcgggaccat
     2221 accaacctgg tagaagggtg cggcccagca atctgacctc gagcggatag agaggatttc
     2281 ccgtcagcac ccagttgcgc acgaaccaga aacctccgga aacgagcgga acaagcgcaa
     2341 tgacgccggc gcggacaatc ttggttcgaa tcgggatgga ctgaagcaca ttctcgacga
     2401 tcgccaccac gaccaagggc ggcataaaga cgacggcggt cggttttgtt gccatggcca
     2461 accctgccgc caacgcactc aggcaatatg ccgcgagttg tccttcagcg cgtccagctt
     2521 gcaagaaaaa ataggcggcg aggaggtaac cagccacaaa gatcgtgtcg acgttaggct
     2581 cgaaggagaa aatgaagagc ggcatcgacg aggcgaacca gcaggtcgcg accagactgg
     2641 ccgctcgagt cgcccccagc acgcgggcac atccaaacgc ggccagagac gccagaaaga
     2701 ggaacggaac ctgcccgatc ctggccaatc ggtcacctcc ccacgtggtc atcagccacg
     2761 cgaaccaaag atccccattg gccgggaaat aggtcgcggc gctctctcca aacggagcgg
     2821 ccaccagcaa caatcgcccg gctttccacc accgcaccgc aaaatagaga tgatagatgg
     2881 ggccgtcgct caccaccttc acacccagaa gcaacgaccg cacgcccaac atgagcgtca
     2941 cccatgacat gagcgcaaca gagaccaatt catcccacga aaacggctcc tttgtcgcat
     3001 catgaggagg atctcgatcg atgctggggc aaaaccacca cagaccgtag ccgatcccag
     3061 caaccagtat cccccaaccc aacagcggtc cttgcgcgag tgcaccactt attcctaata
     3121 tttccgaacc gagcgtgcag gcggtccaga aaaccacggc tgtcgccaat gcgcgattca
     3181 gcccgcgcgg ctgcctgaag ccgtggcggg cgatccaata cgaacccacc aacagcagac
     3241 cattaatgcc caggccgaag agcccagtct ccgcggtcgt ccagatcgaa tcggcgatca
     3301 ctgcctctcc cagtcccaaa cgtcggtttg gctctcgacc ggcccagcca ggccaatgaa
     3361 gttccccgac caaggcaaca aggaacgtcg ttgtgcacga aagcaacgcg gcggcgagcg
     3421 gtcggcttta ccgccctggt ttgccttgag tataatccgt ctgctttact tcccgctcac
     3481 gacagtgatt cagcgactgg gcggtgccct cgaatccgca tcggaccgga cacattgtct
     3541 tcacctgcct gtcgttatgc ccgatctatc ggttgatcag cgcccggccc acctctcgag
     3601 cgactgtttg ataaagaatt tggagctaaa catcgatgat cggtgccaaa ctgggaatct
     3661 ccgattacct ggctcgcgtt tgtgaagaaa tccagcgtct tgatccggcc cagtgcgaaa
     3721 aggtttgcga actcgtggag tctgcctacg acgagggtcg ctttgtcttc atcatcggca
     3781 atggcggatc aggtgcaaac gcctcccatc tttgcgaaga cctggcgaaa tgcactctgc
     3841 acgattttga aaaccagaaa cggctcaaag tgctgagtct gacagataat accgccggaa
     3901 tcatggcctg ggcgaatgac gagggatatg aacgtatctt catcgagcaa ctgaaaaacc
     3961 tggcatcccc tggggatctg cttctcgcaa tttcggggtc gggcaacagc ccgaacattc
     4021 tcaaggcagt gtcgtgggcc aacgaccatg gcctgaccac cgtgggtttt acgggctttt
     4081 ccggcggcaa gctgaagtcg ctcgcccacc acaacttgca cgcaggaatc gacgacatgg
     4141 gaatcgtgga atcgcttcac caagtcgtct tccactggat catcgacgat ctctaccgcc
     4201 gtttctcgct gacggcagcg gttagcaacg gggtcaagac cggttgaacg catgtcggaa
     4261 ttctgggcgc tcgggatcga gatcggcggg accaagctgc agctcggaat tggccagggg
     4321 caaggaagcc tgcttgccct ggatcgactt cgtgtcgatc cgtcgcgggg agcggccggc
     4381 attctcgagc agatcaagga cgcccttccc aggttgctcg cagcggcgaa actcgaccgg
     4441 caccaaatcc aagcggccgg catcggtttc ggaggaccgg tcgacgtcca aaatggacgc
     4501 acccaaaagt ctttccagat agttggttgg gacgatttcc cactcgccgg ctggctcggc
     4561 gagcatctgg aaatttcccg ggtcgtgctg gaaaacgacg cggacacagc cgggttggcg
     4621 gaagcccgct ttggcgcggg cgtgggtcat tcgccgctct tgtacatgac ggttggaagc
     4681 ggaatcggtg gcgcgctcat cgtcaaccgg caaatctatc gcggctcggg ccacggcgcc
     4741 gccgagcttg gtcatctctt ggtaccggaa accactccgt ccgggattcg cctgcgcgag
     4801 ctcgaacaag tggcctccgg ctgggctatc gctgcggccg ctcgtgagct cgctcgacgg
     4861 aaattgcacg acggccgcgg cgagtggatt gtcctctccc aggcccaggg tcgtccggac
     4921 gatatctcga cagatttggt cgcgaacgcg gctcgcgcgg gcgatcccga cgcgctgacg
     4981 attctcgacc acgcctcgac cgctatctcc ttcgccctaa cacaaaccgt cgcgttgctc
     5041 gcgccccaac gaatcgtgat cggcggaggc gtatctctca ttggcgacaa actttggttc
     5101 gacccaatcc gttgtctgct tgaccgggat gtctttggac catttcgagg acgatttgat
     5161 atcgtacccg cggtcctcgg agagcaagtc gttgtccacg gtgcgcttgc gctggcccgc
     5221 gactccattg ccagcgccgg ataatcgacg atgagacaac agataaggtc ctttcacgcg
     5281 tgatgttgca tttttaccac actttacgaa tttattcctt gacaacgcgg gctcacgcat
     5341 acaacaatgt gtgagtgcaa ccgaggtgtt ccgtctgaca cgttagataa aggctagttc
     5401 ctattttcgt gcgttgctgc acggattctc agcagagtgg cttctcctgt cagcatcact
     5461 ccggtttgtt caaaggtctt gcgccaaaag cgactgggtg ttaggacctg gctctcgtga
     5521 ccacaacatc ggctgatttg gtggttagcc ataggtgatg gaaaaacacc acgtcggtgt
     5581 cacgtgaggc agttttctcg cttgcatctg caaacctcga gattagttaa agttgcgttc
     5641 gggatccaca cgttgtattt ttgctattga agggcgattc gtaaacgagt gagccgtggc
     5701 gccagtcacg caaacgcggc gatctcgaat gactgatgtc gtaagttttg gaaaaatgtc
     5761 cgttttatga agaatggccc cgattttgcc tcatttcgga gcgatcagag cgaatgcatt
     5821 gttgaagatc gctctggtgt ggtcgcgttt ctctgacgaa tgattggttg ccgtggttcg
     5881 agatcgttga tacgtcgcaa gcgacgaagt ctgaaccatg tggatttgaa ggtcgtctat
     5941 acggatagag cccgattgag gataattcca gccacggtgg atttgtcatt caggccgaat
     6001 acaattttga tggatcgacg aggtagttca ggatcgacgg tttgatctga tcggcacgtt
     6061 tgggaagcct gcctcgaata acccaaggaa ggcctcttct ctcaacctta aggggctcgc
     6121 catgaccgcg aaactggccg aaacccaact gtggcagcag aacatggctt cgctgatacg
     6181 gtcgggccta ttttcccagg ccaccaccgg cgagatgaat ggtctatata cggtcgtcgg
     6241 agtgtatgtc gacgagacgt tctcggctcc gttggccaaa tactctgatc tccgaagagc
     6301 gactgacgcc gccaacctcg ttaaccagct agcgaaggtt cgacgatcaa ttgagtcgaa
     6361 ctgagcgctc ggatcgcaat cgaaggatcg ccttccaaac catggcctcc tgcggtacaa
     6421 agcgggacgg ccatggtttt ctcttttcga accgcgggtg gtgagccgtc aaccgcagat
     6481 ggctcggcat ccggtcgcca aggttaggat ctcgtgtcat ggaagtcacg agccagcgaa
     6541 gccgcctcag actcgttttg tttgccgtcg ccttggtggg tcatagaccg gcccctccgg
     6601 ttgacgaatg gacggctcct cgccagacgc tacaggccat tggccgacgc ttgagccagt
     6661 caaactccga gcacgccttg accgtgattg cgtcccgcga agacttgatc ctcgaacggc
     6721 tcaaggggca agaacgagct gccctcgctc atggctatct tcgctttcgg gtcgacacgc
     6781 cagtcgtggt cgacgtcgcc gtacccgccg agacaatccc tttctggatc ggcgacctag
     6841 ggtttcgacc tacggacctg acgctcgaga actccgattg ctcgtggacg ctctatcgga
     6901 aaaccttcgg cgccggagtg atcggactgg gaattaacgg tctggatcgc aggccgctgg
     6961 cccattacgc agttttcgtg cggcccgtgc aaaccacggc cgccgatcct cgtcgaccgg
     7021 tcgtgcagct cgactccaga cagacgtccc tgtggaagat caccctggcg cgtgcgggtg
     7081 tgagtgctgc gaacgacact tacaaaccct ttgaatcgct cccggccgag ttaatcgacg
     7141 caatcctgat ccagcccgcg cacggggcac ggcattcggc gctcctggct actggccggg
     7201 tctggaagac ccacgtggtc tcgagctcgc gtccggacca agtcgtcgtc gggtttggct
     7261 cggacccagg gcgggagctg gtgtggactt ggaggacgtc tgccaagatc acatcgtcgg
     7321 ccattcgcat cgccagggct cccgagagca acagaaacaa cccgtcagcc gacgattcca
     7381 aggcgtttcc ggggtctgtg cgtgtcgtca acggcgactc gacgccagtc gaagcgacta
     7441 atctgctcaa tgacgctgtg acccaacggc atcatgtggt ggtggatggg ctccagccgg
     7501 acacggttta tcaatattca ctcggcgatg gtacgcccgg tgggtgggga tcgtgggaaa
     7561 ccgtcaagac ggcacccgat tcatcgcgcg gggcacggtt cctctacctg ggagacgccc
     7621 agaccggact ggaaggatgg ggccggctgc tcaaggcggc gtctcaccaa aatcccgaca
     7681 tcgattgcgt cttgctagcc ggcgacctgg tcgaccgtgg taacgaaagg accaactggg
     7741 atcatttctt cctgcgcgga gccggggtct tcgatcgcgt gccgctcatg ccctgcgtgg
     7801 gaaaccatga atacctcgac atgggacctc gactttatcg cgcattcttc gacctcccgc
     7861 gtaacggccc cacgggaatc gataccgacc tggtttacca gtttgagtgc ggcaacgcgt
     7921 gcatggtcgt gctcgacagc acgctggcgg tttgcgaccc agcggcagcg cgtcggcagg
     7981 cgaattggct cgacgcaacg ctgactcgtt ccaaggcatc gtggaaattc gtcatgtttc
     8041 atcaccccgt ctacccttcg catccgtggc gcgattcgcc ggccatgcgc gaacattggg
     8101 tgccgatctt tgacaagcac cacgtcgatc tcgtgctaca aggccacgat catgcttatc
     8161 agaggacgta ccccttgcgc ggccatcggc gagtcgacac acccggtgac ggcacaatct
     8221 atgtgatcgc cgtctccggc gacaagttcg tcgagcaggc ggcgcgcgac tacgtcgaag
     8281 tgggccgaag tggtatttcg acttatcaga ccatcgagat cgattctccc tcgaaccggt
     8341 tgacctaccg cgcccgcaca gaagatggga cgatcgtcga tgaattgcag atcgagaagg
     8401 cgctacagcc taggaggcat agcgagcccc cgctcagtgg cccgacgccc acgatcgcac
     8461 ccacgctgac acgagcggcg ctcgcgcctg gattgccatg attccgtagg cccatcccca
     8521 ccaaagcacc cacgccagcg tgatccagcc acgccaatcg ggctgccgac cgtcccgcca
     8581 attgagtctc gatttgcttt gcaccgattt cacctcgcga tgatcgtggt tgcgaattga
     8641 tcatatgggc gaatgcggcg actctgcccg gtgaatacgg gaccaaagtc tcatggcacc
     8701 tacgtaaacg cggtcagggc ttaaccccga catgcacgga tggttggcga gcgggcacac
     8761 cttttggtgg cacggttggc aaggtacgcg gtggcggaga accaaggaat gtcgcgacca
     8821 gggccgccac tgtccgggct gattggttcc gctgaacaag atcaccgaca gggtgcccgc
     8881 cgtggcagcc aggtgcgccg ggccggaatc cgaaccgatg aagaggtcag cacgctcgag
     8941 tagcgcggtg gtctgggtaa ccgtcagact gccggtccag tcccggagcc ggtcatgcgg
     9001 ttctagaaca cgggagaggt gtgcgtcttc gataccaccg actaccacga tacgccagcc
     9061 gctttcgatg aaccgatcga tcaacgcttg ccatgagccg atcggccagc gcttggcggg
     9121 gttgccagcc ccgagatgca cggccagcag aggcggacgc gaggtaaact gatcggcgtg
     9181 caggtagtcc gcgtcggcgg cgatcgagtc gatcgaagtg gcacggcctt ccaggccgtg
     9241 tccggattcg ggcaaaacac ccgtgccgcg gtgagtcgtc gataactttg tttcccgtcg
     9301 gtcgccgaca cctgcgcggt gcagaaccgc agccgtagcg taagattcgg atcggcgcgt
     9361 gctgcggcgc ggccattcct ccgacagccg gcgggcaatc aaaatccgat cctggtcgca
     9421 tacgtggacg tcgacccgtg tcagctcgtc gggattaatc cccaggc
//
LOCUS       DATAQL010000597         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3724795, whole genome shotgun sequence.
ACCESSION   DATAQL010000597 DATAQL010000000
VERSION     DATAQL010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724795"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2209)
                     /locus_tag="VHS97_10460"
     CDS             complement(<1..2209)
                     /locus_tag="VHS97_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3448666.1"
                     /translation="MKTVAKIGLALGLMVALALGVSFALSDPTLGRSRESGTQQVHRS
                     GKPKAARKAWNTRADGSPMLYGDGWFDDSGYFFAVLFNGAMADPLSLEQIGTALAGRG
                     RRGIDHLRTVVESVPRDDPDASRQISELHLSIGGLHMYEGDFAAAAREFEAGRDSLGK
                     VDDLTWANYEALLGIVALRRGEVENCVACCNEESCIFPLAAKAVHSRTSGSREAVRRF
                     TAYLEKRPEDIGVRWLLNVAAMTLGEYPDKVPAKYLIPLDPFQSRVDLGRFPNVAARI
                     GLDPGGNMAGGSILDDFNGDGLIDVFTSTANPTRGARLFVNRGDGTFEDRSLKAGLAG
                     QIAALNCNQADYDNDGDLDVYLMRGGWENAIRPSLLRNEGDGRFTDVTVSAGIATPIA
                     SQSAAWGDYDNDGDVDLFVAGEYVPQQPDFRYPGATLPIPDPRNCCRPYRNNGDGTFT
                     DVAEFAGVLNERKAKGSAWGDYDNDGRLDLYVSNSGQENRLYHNNGDGTFTDLAPELG
                     VMEPIKSFSCWFFDYDNDGWQDLFVNGFSGTLADAIRSHLGQPTAGERPRLYHNLGPR
                     GFRDVTKEAGLDRVMLVMGSNFGDIDNDGYLDMYLGTGQPAYFYVVPNVMLKNIDGTR
                     FEDVTTCTGTGHLQKGHGISLADWDRDGDVDIFLSSGGATPGDEAHNILFQNPGHGNH
                     WINVKLVGTKTNRAGIGARVRVDLPKSSGGVMSRYRVIGSGSSFGGNSLATTIG"
BASE COUNT          437 a          738 c          771 g          484 t
ORIGIN      
        1 gcccgattgt ggtggcgagg ctgttgccac cgaaactcga cccgctgcca atcacacgat
       61 accgtgacat gacgccgccg gacgacttgg gcagatcgac ccggactcgg gcgccgatgc
      121 ctgcgcggtt ggtcttggtt cctacgagct tgacgttgat ccagtggttg ccgtgcccgg
      181 ggttctgaaa caggatgttg tgcgcctcat ctcctggggt tgcgccgccg gacgagagga
      241 agatgtcgac gtctccatcg cggtcccagt cggccaggga gatcccgtgt cccttctgga
      301 gatgcccggt gccggtgcac gtggtcacgt cctcgaaccg cgtcccgtcg atgtttttga
      361 gcatcacgtt gggtacaacg taaaagtagg ccggctggcc ggtgcccagg tacatgtcga
      421 ggtagccgtc gttgtcgatg tcaccaaagt tggagcccat gacaagcatc acacggtcga
      481 gcccggcctc cttggtcaca tcgcgaaaac cccgagggcc aaggttgtgg tagagccggg
      541 gtcgttcgcc ggcggtcggt tggccgagat ggctgcggat ggcgtcggcg agagtccccg
      601 agaaaccgtt gacgaaaagg tcttgccagc cgtcgttatc gtagtcgaaa aaccagcacg
      661 agaagctttt gatcggctcc atgacgccca gttcgggcgc gaggtcggtg aaagtgccgt
      721 cgccgttgtt gtgataaagc cggttttcct ggcctgagtt ggagacataa agatcgagcc
      781 ggccgtcgtt gtcgtagtcg ccccatgccg atcccttggc cttgcgctcg ttgagaactc
      841 cagcgaattc agcgacgtcg gtgaaggtgc cgtcgccgtt attgcgatag ggccggcagc
      901 agttgcgtgg atcagggatg ggcagggtgg cgcccgggta ccggaaatcg ggctgttgcg
      961 gaacatactc gccggcgaca aacaggtcga cgtcgccgtc gttatcgtaa tcgccccacg
     1021 ccgccgactg cgaggcgatg ggcgtcgcga tcccggcgga cacggtcacg tcggtgaagc
     1081 ggccgtcccc ttcattgcgc aagagcgacg ggcggatcgc gttttcccag cctccccgca
     1141 tcaagtagac gtcgaggtcg ccgtcgttgt cgtagtcggc ctgattgcag ttgagcgcgg
     1201 cgatctggcc ggccaacccg gctttaagcg agcgatcctc aaacgtgccg tctccacggt
     1261 tgacgaacag gcgagcgccc cgagtggggt tggcggtact ggtgaagacg tcgatcagcc
     1321 cgtcgccgtt gaagtcgtcg agaatgctgc cgccggccat gttgcccccc ggatcgaggc
     1381 caattcgggc agcgacgttg ggaaaccgac ccaggtcgac ccgtgactgg aaggggtcga
     1441 gcgggatcag gtatttcgcc gggactttgt cggggtactc accgagcgtc atggcggcaa
     1501 catttaagag ccatcggacg ccgatatcct cgggtctttt ttctagatac gcggtgaagc
     1561 ggcgaaccgc ctcgcgcgat ccgctcgtcc tgctgtgtac cgccttggcg gccagaggaa
     1621 agatgcaact ttcttcgttg cagcatgcga cgcagttttc gacctccccg cgccgcaggg
     1681 cgacgatccc cagcagggct tcgtagtttg cccacgtgag gtcgtcgact ttgcccaggg
     1741 agtcgcgtcc ggcctcgaat tctcgcgcag cggcggcaaa atccccctcg tacatgtgca
     1801 gcccgcctat cgagaggtgt aactcggaga tctgcctgga cgcgtctgga tcgtcgcgag
     1861 gcacactttc cacaacggtg cgcagatgat cgatcccccg gcgcccgcga ccggccaggg
     1921 cagtcccgat ttgctcgagt gaaagggggt cggccatcgc accgttgaag agcacggcga
     1981 agaaatagcc gctgtcatca aaccagccgt cgccatagag cataggcgac ccatctgccc
     2041 tcgtgttcca tgctttgcgg gctgccttcg gcttgccgga gcggtggacc tgctgcgtcc
     2101 cgctctctcg gctgcgaccc agagttggat cggagagcgc aaacgagact ccaagcgcga
     2161 gagccaccat caggccgagc gccaggccaa tcttcgcaac cgttttcaac catccgttca
     2221 ttgctgaccc ttcgatcccc acgcctgtgg cgctcgttcg agcaggcgac taatactcga
     2281 tcacgagtga gaccgcctgg gtgttaccgt gctcttgtcg acgattttac caggttttca
     2341 gttttgaaga acggcacggt gagtagacac cgagcccgaa catcctgatg tgccacgact
     2401 gccgccggat taatcgcctt cgatccattc
//
LOCUS       DATAQL010000598         3648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2045344, whole genome shotgun sequence.
ACCESSION   DATAQL010000598 DATAQL010000000
VERSION     DATAQL010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045344"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1140)
                     /locus_tag="VHS97_10465"
     CDS             complement(184..1140)
                     /locus_tag="VHS97_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448667.1"
                     /translation="MAEGQGRDSRRLSPAGAGQTPAPTITQPVMFNTPEADRILSELQ
                     IFPPDNPWNEDISKRPVAPNSTNLIASVGADKSLAYNFDMAFILVPPDQKRVPVQINQ
                     YPDESDPGPYPVPDNAPIENWPLVGQPLDTIQRLGSGDRHMLILDPVNMTLFEFYTAR
                     KTAAGWVAAQASIFDLKSNKLRPDGWTSTDAAGLPILPSVVRYDELEHGKVTHAMRFT
                     IRNSRRAYVYPATHFASSKSDMNLPRMGERFRLRQNFNITGFSPHTQALLMGLKKHGM
                     FVADNGGDWRISVAPDSRIKGFDELRRVKGRDFEVVQPTGEK"
     gene            1308..3311
                     /locus_tag="VHS97_10470"
     CDS             1308..3311
                     /locus_tag="VHS97_10470"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX3448668.1"
                     /translation="MRPLSRRDFLDLALTVPAAGLASTTAMAALPGASPADVHQQILE
                     LAAVQEHERRARFAAVKNKGDLDVLGSSLRQTFLKLVGDLPDRQGVPSVRKAGTIEAD
                     DYVVDKLVFESFPGYFVSALLYKPKKITTPVPGVLSPCGHSVTGKAAGPYQILHINLA
                     KRGYVVLTYDPVGQGERSQFWDAAKGRSRFNLSCGEHAVLGNPLYLLGTNLARYRIFD
                     GLRGLDYLVSLPEVDASRIGCVGNSGGGTLTAYIAALDSRVRAVAIGCYITTLRRRMG
                     NRIQQDPDADPEQDIFGFVSEGIDHAGLLAMIAPRPTLLGFARFDFFPIEGARETFDE
                     ARRLYEVAGAGDRIDRVEAAERHGLSLPLRTATYEWFGRWLSGNREAAPATEFAVSPR
                     PAQELLVCKDGQVSLSFRSRPLLPLALEQFDGTKRPERIPLRQLLNLDTERADPHITR
                     VSPGSSRSATAVLCVNGNETRDWRDETRFFDGLARHGLAVTVVDPRGVAQRRPGLAIF
                     GRNYADPLVGVEENIAYNAFLVGKSLLGMRVTDVLVAVRKLLDQDKPGRIVLCARRDA
                     ALTACFAAAVEPAIESVATEDMVLSFRGLFSSSAAPINAASILPDLLRRFGDIADVLA
                     EIAPRRILVAAGVGTALPSARHISAVSDRFSANSRLLTDWIGE"
BASE COUNT          663 a         1044 c         1172 g          769 t
ORIGIN      
        1 atcgaggtga tctcttggaa acagtttcgc gcgttgaccg aaaaggccgg cggcgccaag
       61 acgtctgcac cggcatcagg tcgctagatc aagccgcgcc attccctcgt tgagcagggt
      121 agagccatga gaatcgaccg gcgatccaga gaacattgtt catcgctcat cgctgcccgg
      181 tcattacttc tcgcccgtcg gctggacaac ctcgaagtct ctccccttga ctctccggag
      241 ctcgtcgaaa cctttgatcc ggctgtcggg cgcgaccgag atgcgccagt cgccgccgtt
      301 gtccgcgaca aacatcccgt gttttttcag ccccatgagc agcgcctgag tgtgcggcga
      361 gaatcccgtg atgttgaagt tttggcggag ccgaaatcgc tcgcccatcc gcggcagatt
      421 catgtcggac ttgctgctgg caaaatgggt ggccggataa acgtatgccc ggcgcgagtt
      481 gcgaatcgtg aaccgcatcg cgtgcgtgac cttgccgtgc tcgagctcat cgtatcgaac
      541 gacggagggg agtatcggta ggccggcggc atcggtggaa gtccagccgt ccgggcgcaa
      601 tttgttcgat ttgaggtcga agatcgacgc ctgcgccgca acccaacccg cggcggtttt
      661 gcgggccgtg taaaactcga agagtgtcat gttgaccgga tcgaggataa gcatatgtct
      721 gtcaccactg ccgaggcgct ggatcgtgtc gagcggctgg ccgacgagcg gccagttttc
      781 gatcggcgcg ttgtcgggga ccgggtaggg cccagggtcg gattcatcgg ggtattggtt
      841 gatctggacg ggaactcgct tctggtcggg ggggacgagg atgaacgcca tgtcgaagtt
      901 atacgccagg ctcttgtcgg caccgaccga cgcgatcaag ttcgtggaat tgggggcgac
      961 gggccgcttc gagatgtctt cgttccaggg gttgtccggc ggaaatatct ggagttctga
     1021 gagaatgcgg tccgcttcgg gtgtgttgaa catcacgggt tgcgtgatcg tgggtgcggg
     1081 agtctggccg gcgcccgcgg gggagaggcg acgactgtct ctgccctggc cttctgccat
     1141 gatgaaggga ccgccatcac cccccatgaa aagaaagaga acacaaggca atagcagcac
     1201 ggatctgcag gcgattatca tggcttcccc attggacatg cgccgtcaaa gaactcgaag
     1261 acgtgatcgt caggtcgtgc gttgctctcg tcaaggagtt accagtcatg cgtccgctgt
     1321 ctcgacgcga tttcctcgat ctcgctctga cggtccccgc cgccggcctg gcttccacca
     1381 cggcgatggc cgccttgccc ggggcgagtc cggccgacgt ccaccagcag atcctcgagc
     1441 tggccgctgt tcaggaacac gaacgccggg cacgcttcgc ggctgtgaaa aacaagggcg
     1501 atctggacgt gctcggttcg tcgctgcgcc agacgtttct taagcttgtc ggggatctac
     1561 ccgaccgtca gggcgtgcct tctgtacgga aggcggggac gatcgaagcc gacgattacg
     1621 tcgtcgacaa gcttgttttc gaaagctttc caggctattt cgtatcggcc ttgctatata
     1681 aacccaagaa gatcacaacg ccagtcccgg gtgtcttgag tccctgcggc cactcagtga
     1741 ccggcaaggc tgcggggcca tatcagatcc tgcatatcaa cctcgcgaag cgcggctatg
     1801 tcgtcttgac gtatgacccc gtcggccagg gggagcgcag tcagttctgg gatgcggcga
     1861 aaggccgatc acgcttcaat ttgagctgcg gtgagcacgc cgtcttgggt aatccgcttt
     1921 atctcctcgg cacgaatctg gcacggtatc gaatcttcga cggccttcgg ggactggact
     1981 acctggtctc gctaccggaa gtggacgcat cgaggatcgg ttgtgtcggc aactcggggg
     2041 gtggcacgct caccgcctac attgcagccc ttgattcccg tgtaagggct gtggcgatcg
     2101 gttgctacat cacgaccttg cggcgccgga tgggcaaccg gatccagcag gatcccgatg
     2161 cggatccgga gcaggacatc tttggatttg tcagcgaagg gatcgaccat gctggcctgc
     2221 tcgcaatgat cgccccgcga ccgaccttgc tgggtttcgc gcgctttgat ttcttcccca
     2281 tcgaaggcgc tcgcgaaacg ttcgacgaag ccaggcggct gtatgaagtc gcgggcgcgg
     2341 gcgatcgcat cgaccgagtc gaggccgccg agcgacacgg tttgtcgctg cccttgcgca
     2401 cggcgactta cgaatggttt ggtcgctggc tatctggaaa ccgcgaggcg gctcccgcca
     2461 ctgaattcgc ggtcagcccg cgacctgcgc aggagttgct cgtctgcaag gatggtcagg
     2521 tcagcctctc gttccgatcg cggccgctct tgccgctggc tctggaacaa ttcgacggca
     2581 cgaaaaggcc cgagaggatc cccctgcggc aattactcaa ccttgatacc gaacgggcgg
     2641 atccgcacat cactcgggta tcgccgggtt cgagtcggag tgcgacagct gttctctgtg
     2701 tcaacggcaa cgagacgcgc gattggcgcg acgagaccag gtttttcgat ggactggcgc
     2761 ggcacgggct tgctgttacg gtcgttgatc cgcgcggagt tgcgcagcgc cgccccggac
     2821 ttgcgatctt tgggcggaac tacgccgatc cgcttgtcgg cgtagaggaa aacatcgctt
     2881 acaacgcatt tctggtgggg aagtcgcttc tgggcatgcg ggtgaccgat gtgctcgtcg
     2941 cggttcgaaa gctgctcgat caagataaac ctgggcgcat cgtcctctgc gcacgtcgcg
     3001 atgccgcgct cacggcctgt ttcgccgcgg ccgtggaacc ggcaatcgag tcggtcgcga
     3061 cggaggacat ggtcctgagc ttccgtgggc tcttttcgtc gagcgcggct ccgatcaacg
     3121 cggcgagcat tctgccggac ttgctgcggc gctttggcga tattgccgac gtgctggccg
     3181 agatcgcacc gcggcgaatc ttggtcgcag cgggtgtcgg tacggcgttg ccgagtgcgc
     3241 ggcatatatc ggcagtctcc gatcgattca gcgcgaattc gcgactattg actgattgga
     3301 tcggggagtg agaattagcg acccagacag ttggacttat gaaggattcg tgccgtgcgg
     3361 ctacccacga tgacgacgcg gcgctggatg atcgtggcgg cggtcgtcgg acttgcattc
     3421 ggaattgcga cctggatggc gggggtaacg ggtgggcaag gagtcgtact cgtattggca
     3481 gtgagcatgt taggatcctt tacaattcca ttattcttat ccgtcgtcca gttccctgac
     3541 actgatcgag gtcgacatcg cacaggcggg acaagaagac aatgattcca attctgcgtg
     3601 ctccgaaaat ctggctgtcg ccaggccggt tgtttcgcga cacactcg
//
LOCUS       DATAQL010000599         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4183595, whole genome shotgun sequence.
ACCESSION   DATAQL010000599 DATAQL010000000
VERSION     DATAQL010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4183595"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VHS97_10475"
     CDS             complement(<1..1135)
                     /locus_tag="VHS97_10475"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3448669.1"
                     /translation="MANVVSKSSQTPSSILDSADQSIYEVTTKADGPAGSLPLTDEML
                     TDWPSGDLFGLTQNAGMGWTATEVARDPYLILSTQGGLRGPDGQAIALGYHTGHWEIG
                     LLVKEAAEELRRLDVVPFAGMVSDPCDGRTQGTAGMMDSLPYRNDAAIVFRRLIRSLP
                     RRKGVLGVATCDKGLPAMMMALAGSKHLACVLVPGGVTLPPRDGEDAGKIQSIGARYA
                     HGELTLPEAAELGCRACASPGGGCQFLGTAATSQVVAEALGITLPHAALAPSGQPIWR
                     DMARRSARALVAMAARNFTLADVLTQSAIKNAMVVHAAFGGSTNLLLHIPAVAFAAGL
                     RRPTVEDWHEVNVKVPRLVSVLPNGPYYHPTVRAFLAGGVPEVM"
BASE COUNT          292 a          500 c          553 g          292 t
ORIGIN      
        1 gcatgacttc cggcactccg ccggcgagaa aggcgcgcac ggtcgggtgg tagtaggggc
       61 cgttggggag gacgctgaca agccgcggaa ccttgacatt gacttcgtgc cagtcctcga
      121 ccgtgggccg gcgcaggccc gccgcgaagg cgacggccgg tatgtggagc agtaagttgg
      181 tcgagccgcc gaacgcggcg tgcacgacca tggcgttctt gattgcggac tgagtgagca
      241 cgtcggcgag agtgaaattt cgcgcggcca tcgcgaccag cgcgcgtgcc gatcggcggg
      301 ccatgtcgcg ccagatcggc tgaccggatg gggctaacgc ggcgtgtggg agcgtgatcc
      361 cgagcgcctc ggcgacgacc tgcgaagtgg ccgcggttcc cagaaactgg cagccgccac
      421 cgggactggc acaggcccgg cagcccagct cggcagcctc gggcaaggtg agctcgccgt
      481 gggcgtagcg ggcgccgatc gactggatct tgccggcgtc ttcgccgtcg cgcgggggca
      541 aagtgacacc gccggggacc aaaacgcagg ccaggtgctt cgagccggcc agggccatca
      601 tcatggcggg cagccccttg tcgcaggtcg cgaccccgag tactccttta cggcgcggca
      661 gcgaccgtat caggcgccga aaaacgatgg ccgcgtcatt gcgatacggc aagctatcca
      721 tcatccccgc ggtcccctgg gtacggccgt cacaggggtc ggaaaccatg ccggcaaatg
      781 ggacgacgtc gagccgccgc agctcctcgg cggcttcttt gaccaacaga ccaatctccc
      841 agtggccggt gtgataacca agagcgatcg cctggccatc gggcccgcgc aggccccctt
      901 gagtgctcag aataaggtat gggtcgcggg cgacctccgt ggcggtccaa cccatacccg
      961 cgttctgggt caagccaaag agatcgccgg agggccagtc ggtgagcatc tcgtcggtca
     1021 atggaagtga gccggccggt ccatcggcct tggttgtcac ttcgtaaatc gattggtcgg
     1081 cggagtcaag aattgagctt ggtgtctggc tggacttcga cacgacgttt gccatgatga
     1141 atgccgttct cgtgctttgt gcattgtggt tgtggtggcg cgccggcaaa gtgcggcgcc
     1201 aggtcgcgcc tcttcggtcc gtgcggacgt tccacttcat gatgcattga tgaggcggaa
     1261 agcaatagtc gagatccaga tcaatggcct gaaagaggtc cgttcagctt tgatattcgc
     1321 agtctacggc tgtgcgaccg cgcatcccgg gtcggcatga agtgtgcgtg aatgcgtttg
     1381 tagggtggtg cagagcctcg atttatcgag gcgaggccca ccgaaaccgc catggcactc
     1441 ggtgggcctc acgattccgc ggatcgttcg gccggccaag gctctgggtg ggcctcacga
     1501 ccctgcggat cgttcggccg gccaaggcac tcggtgggcc tcacgatcct gcggatcgtt
     1561 cggcaccacc ctactcaacg aactcgtcaa gagtctgccg cctctcgatc accatgacaa
     1621 tgacattact ccgaacg
//
LOCUS       DATAQL010000600         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4640764, whole genome shotgun sequence.
ACCESSION   DATAQL010000600 DATAQL010000000
VERSION     DATAQL010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4640764"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VHS97_10480"
     CDS             complement(<1..606)
                     /locus_tag="VHS97_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX3448670.1"
                     /translation="MLEADLITHRSWSRMSAATALLLFALSSLPVGLAQAPPGSQSPS
                     AVGAAEKATTEAAKTLFARENLIAWCIVPFDSKKRGPEERAAMLQKLGFKHFAYDWRT
                     EHISSFDAEVEALNVHGVALDAFWVAPGELNRESRIILDLLKRHGKKAQLWVLLDLGN
                     DRAQGIEQERRVEAAALKLRPLAEEAGKIGCSLSLYNHGGWF"
     gene            complement(1023..>3128)
                     /locus_tag="VHS97_10485"
     CDS             complement(1023..>3128)
                     /locus_tag="VHS97_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3448671.1"
                     /translation="GRLIRDLERIWDVPSSELRQQVVSQLGHDSPLVRAAACRTLGRI
                     GDETALSGLAKCLGDETKVVRRAAAEALRRIGNRSSGSHRAGETQVQLRIVAELTQAL
                     TSADDRTRRGATRLFAAHFRELSQETALLDPLLQLCGDRDPVVAMQAIKALWKWWYWR
                     ADPGVRNRIEDRLIGALAEPRHPWVRRNLIEALYIIGDDNIRYLYQNWVPSLATIESR
                     RRATAGQHETVNRLGAKYVTVLESGTVLQREGVLRAMSEFFERPVLGGRIGNDLEPML
                     FYDDMVPRIAAALVSQLADPDPEIRRLALEALVTVRGDRSADLARSVARRLGESDDAV
                     RTWAITMTKEFPLKINRGQSDPATIALVDELMTQSGSEAQAAALAIIGRLGPVTGIDP
                     SSDPAAKVRTRLNATDAIVRAAAYNALRSFPTLWAEPLVHRAIESGLGDSDPQVRSAA
                     VRLALEPKAKIAESALRKALDDPAPAVRIALLERIASEAALTEDLRLLAVVSNSLVDE
                     NGGVCERALQLIQSKPALAANAAIENGLRELSQSPRTAERQREIAKALLATRGRSSSG
                     AGDAGERLDLTYFKAKILPIFNRIGEDGQNCMGCHRSHTILKMVPPGKDGEWTPETAR
                     DNFRAALRVINLARPADSVILGKPTWEAAEEAEAQNDPTKKAHAGGVRFEKNSREYQT
                     LLDWINGARLPGGSNSAIR"
BASE COUNT          546 a          966 c         1004 g          612 t
ORIGIN      
        1 aaaccagcca ccgtggttgt atagcgagag cgagcagccg atcttgccgg cctcttctgc
       61 taatgggcgc aactttagag cggccgcctc gactcggcgc tcctgttcga ttccttgcgc
      121 gcgatcattt ccgaggtcga gcaaaaccca gagctgggcc tttttcccgt ggcgtttcag
      181 caggtcgagg atgatgcgcg actcgcgatt aagctcgcct ggcgcgaccc aaaacgcgtc
      241 aagcgcgaca ccgtgcacgt tcagtgcctc gacttcggcg tcaaaggaag agatgtgctc
      301 ggtccgccag tcgtaggcga agtgcttgaa tcccagcttt tggagcatgg ctgcccgctc
      361 ttcgggcccg cgtttcttgc tgtcgaaggg cacgatgcac caggcaatca agttttcgcg
      421 cgcgaagagc gtcttggcgg cttcggttgt tgctttctcg gcagcgccca cggcagacgg
      481 cgactgtgag cctggcggcg cctgggcgag cccgaccgga aggctcgaca gagcaaaaag
      541 caacagtgcc gtcgccgcgc tcatgcgcga ccatgagcgg tgggtgatca agtctgcctc
      601 gagcatcgtc atgtggtcct attgaagcgc aagaatagat tgacgtcgga tgtccgacat
      661 ggggagggcc aacagcctcg tcgattgcgg gtgaggggca acgccgttaa gcatttcgtc
      721 tggggcgacc gatggcttgt tcccacgctc ccgcgtggga acatcggtcc gcgacgctca
      781 gcgtcgcttg gctgcgcttc gccgtcggct tgacgaaacg acgcggagcg tcctatgaaa
      841 cgttcccacg cgggagcgtg ggaacgaggg aaaatgttta acggcgttgg gtgaggggga
      901 gcttgctctt gggttgtcgt cggcatcgtt atgagcgtgc tggccccctt gctttcactc
      961 gcgagtctga ccgtcgtgtt cttactggga aggtacacgg tggtcgtccc tcggtcccaa
     1021 atttaccgta tggcgctgtt gctcccgcca ggcaggcgcg ctccgttgat ccagtcgagt
     1081 aatgtctggt attcgcggga gtttttctcg aatcgcacac cgccggcgtg ggctttcttg
     1141 gtggggtcgt tctgcgcctc ggcttcctca gccgcttccc aggtcggctt gcccaggatg
     1201 acgctgtcgg ccggacgggc gaggttgatg acgcgcaagg ccgcgcgaaa attatcccgc
     1261 gcggtctcgg gcgtccattc gccgtccttg cctggtggga ccattttcaa aattgtatgc
     1321 gagcggtgac agcccatgca gttctggccg tcctcgccga ttcggttgaa aatcggcagg
     1381 attttggcct tgaagtaagt taggtcgagc cgctcgcccg catcgcccgc gcctgagctg
     1441 gagcgaccgc gcgtcgccag cagtgccttg gcgatttccc gctgccgttc ggcggtcctc
     1501 ggcgattgcg agagctcgcg caaaccgttc tcgatcgccg cgttggccgc gagcgcgggc
     1561 ttgctctgga tcagttggag agcccgttcg caaacgcccc cattttcgtc gaccagtgaa
     1621 ttgctcacca ccgcaagcaa gcgtaggtct tcggtgagtg cggcttcgga ggcgatccgt
     1681 tcgaggagtg cgatgcgtac ggcgggagcc ggatcgtcaa gcgccttgcg caaggcggat
     1741 tcggcgatct tcgctttcgg ttcgagcgca agccggacgg cggcagagcg gacttgggga
     1801 tcactgtcgc ccagtccgct ctcgatcgct ctatgaacca gcggctctgc ccagagcgtg
     1861 gggaaagacc gcagcgcgtt ataggcagca gcgcgaacga tcgcatcggt cgcgttcaac
     1921 cgcgtacgga ctttcgcagc cgggtcactg ctcgggtcga tgcccgtgac aggtcccagt
     1981 cggccgatga tggcaagagc ggccgcctga gcctccgagc cggactgcgt cataagctca
     2041 tcgaccagcg cgatggtggc gggatcgctc tggccgcggt tgattttgag gggaaattcc
     2101 ttggtcatgg tgatcgccca ggtgcgaacg gcatcatcag attcacccag tcgacgcgcg
     2161 acggatcgtg caaggtcggc cgaacggtcg ccccggaccg tgaccaacgc ctcgagagcc
     2221 aaccgtcgga tctccggatc ggggtctgcc aactgagaca cgagcgcagc cgcaatccgg
     2281 ggcaccatgt cgtcgtagaa gagcatcggc tcaaggtcat tgccgatccg gccccccagc
     2341 acgggccgct cgaaaaactc cgacatcgcg cgcagcaccc cctcgcgttg cagcacagtg
     2401 ccactctcga ggaccgtcac atactttgcc cccaggcgat tgaccgtttc gtgctgtccg
     2461 gccgtggcgc ggcgccggct ctcgatcgtg gccagcgagg gaacccagtt ctgataaagg
     2521 tatcggatat tgtcgtcgcc aatgatataa agagcctcga tcagattgcg ccgcacccag
     2581 ggatgacgcg gctcggccag agcaccgatc aacctgtctt cgattcggtt tctcacgcca
     2641 ggatcggcgc gccagtacca ccatttccac aacgctttga tcgcttgcat ggcgacgacc
     2701 ggatcgcgat cgccgcacag ttgcagcagc ggatcgagca aggcggtttc ctgagacagc
     2761 tcgcggaaat gggcggcgaa caaccgcgtg gcaccgcgac gagtgcgatc atccgcggag
     2821 gtgagagcct gcgtgagctc ggcgacgatt cgaagctgga cttgggtctc acccgcccgg
     2881 tgcgaaccgc ttgagcggtt acctatccgc cgtaacgcct cggccgcagc ccggcgcacg
     2941 accttggttt catcccccag gcacttcgcc aagccggaga gcgccgtctc atccccgatg
     3001 cgtcccagag tccggcatgc ggccgcgcgg acaagaggag agtcgtggcc aagctgtgag
     3061 accacctgct gccgcagctc ggaactgggt acgtcccaaa ttcgctcgag gtcgcggatg
     3121 agtcgacc
//
LOCUS       DATAQL010000601         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4334762, whole genome shotgun sequence.
ACCESSION   DATAQL010000601 DATAQL010000000
VERSION     DATAQL010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4334762"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VHS97_10490"
     CDS             complement(<1..369)
                     /locus_tag="VHS97_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3448672.1"
                     /translation="MYRFQCRLKRPAFTLIELLVVIAIIAVLIALLLPAVQSAREAAR
                     RIQCTNNLKQLGLGLANYESANGALPPTSILYGVEKGSKVRQFKSNWSVLARITPFLE
                     ESPLYNAINFDYKNSDVQNTT"
     gene            complement(392..1396)
                     /locus_tag="VHS97_10495"
     CDS             complement(392..1396)
                     /locus_tag="VHS97_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448673.1"
                     /translation="MADTDSVYEAVTTHEMYAPDRRNGDRARPAPARRAVTTTRNRYH
                     LVLKLIRRGHLFTGLFMTPWIFLYGVSGFLFNHPGAFSDQEVRSFGQTESAGTGLEDF
                     PGAATIAARVVEAINARSSDRALRLVEPEAATFSRDMIATVKGDSAEYRIQLALESGR
                     GTVSPVRPKKPRIKEDTVAGRVELTDPARDRLSRAIPALAEKLGLNPKSVSVKSAPEV
                     VFSAESPGKLYRLAYNLQTDVVTSHKDGEESEGLTTRAFLTQLHKKRTFPARVDSRWF
                     WAVSVDATSALMLFWGISGLIMWWQIKSTRKWGLLVLAASGLTAAALALALHRVLNYA
                     "
     gene            complement(1610..>1813)
                     /locus_tag="VHS97_10500"
     CDS             complement(1610..>1813)
                     /locus_tag="VHS97_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448674.1"
                     /translation="RCVHALAQLSVQHYLTIDSHDLLVLGVGSRCAKWPASNRPSGTR
                     LADKKKIFIFGRSRTGKNAASQE"
BASE COUNT          371 a          519 c          557 g          366 t
ORIGIN      
        1 ggtcgtgttc tggacgtcgc tgttcttgta gtcgaagttg atggcgttgt acaacggcga
       61 ctcttcgagg aagggcgtga tccgcgcgag cacgctccag ttcgacttga actgccgcac
      121 cttgctcccc ttctcgacgc cgtagaggat cgacgtgggg ggtagggcgc cgttggcgct
      181 ctcgtagttg gccagcccta ggccgagctg cttaaggtta ttggtgcact ggatgcgccg
      241 ggcggcctcg cgggctgact ggacggccgg caacagaagc gcgatcaaca ctgcgatgat
      301 ggcgatgacg accaacaact cgatcagggt gaacgcaggc cgcttgaggc gacattggaa
      361 tcgatacata aacgatctcc tcgtaattag gtcaggcgta attcagcact cgatgcagag
      421 ctagcgccaa tgcggccgcg gtcaagccgc tcgccgcaag gaccaacaag ccccacttcc
      481 gtgtcgactt gatctgccac cacatgatta ggccggagat gccccagaag agcattaacg
      541 cgcttgtggc atcgacgctg acggcccaga accagcgtga atcgacccgc gcgggaaagg
      601 ttcgcttctt gtggagctgc gtgaggaatg cgcgggtcgt cagaccttcc gactcctcgc
      661 cgtccttatg ggatgtgacg acatcagtct ggaggttata agcgaggcga tataactttc
      721 ccggtgactc ggcggagaag accacctcgg gagcggattt gacagagacg gacttcggat
      781 ttagacccag cttctcggcc agggccggta tcgcacgact cagccgatcc cgggcggggt
      841 cggtgagctc gacgcggccc gcgacggtgt cctccttgat tcggggcttt ttcgggcgaa
      901 ccggagagac cgtcccccgt ccggactcga gcgcgagctg gatcctgtat tcggcagaat
      961 cgcccttcac ggtcgcaatc atgtcgcggg aaaaggtagc ggcctcgggt tcgacaagtc
     1021 tgagcgcccg atccgatgac ctggcgttga tcgcctcgac cactcgggcc gcgatcgtgg
     1081 ccgcaccggg gaaatcttcg agcccagttc cggccgattc agtctggccg aacgaccgga
     1141 cctcttgatc ggagaaggct cccgggtggt tgaagaggaa cccgctgacc ccgtatagga
     1201 agatccacgg ggtcataaaa agccctgtaa ataagtgacc gcgacgaatc agcttcaata
     1261 cgaggtggta tcggttacgt gtcgtcgtca ctgcccgccg tgccggggcg ggacgagccc
     1321 gatctccatt gcgacggtcc ggagcataca tctcatgagt cgtcacagcc tcatagacgg
     1381 aatccgtgtc cgccatcggt ttctccgttg gtaccgccag tcagtgtcgt aatgacgcga
     1441 gggaccgatc gcggaaccgc tcctccgcgg ctccagcctc gttgcggggt cgattgtgag
     1501 caggacgtat cggcgactca agtgaataat agttgaataa tccaagaatg cagattactt
     1561 actctaaagg aatccttagt gtcgaccgaa gtcgatacaa ggcgtccagc tactcttggg
     1621 atgcggcatt cttcccggtg cggctgcgcc cgaaaataaa gattttcttc ttatcggcga
     1681 gccttgtacc cgacggccgg ttggaggctg gccacttcgc gcagcgcgat ccaacgccaa
     1741 gcaccagtag gtcatggcta tcaatagtta agtaatgttg cacgctcagt tgtgcgagag
     1801 catgaacgca tcg
//
LOCUS       DATAQL010000602         6944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4334882, whole genome shotgun sequence.
ACCESSION   DATAQL010000602 DATAQL010000000
VERSION     DATAQL010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4334882"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VHS97_10505"
     CDS             <1..519
                     /locus_tag="VHS97_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448675.1"
                     /translation="MGSMMGMRGMASMMMGGGTDPRSSAKAANRQIRPQIAALAAGLA
                     AREGDPLSKVVLKKLEEPISMSFANETPLDDVLKYIRQATTTPTYAGIPIYVDPKGLE
                     EATAALDSTVSFDLEHVPLKTTLRLMLKQIGLAYCVRDGVLIISSVQGVNEELNEAQS
                     EIDGIKPQGGIQ"
     gene            complement(534..2240)
                     /locus_tag="VHS97_10510"
     CDS             complement(534..2240)
                     /locus_tag="VHS97_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3448676.1"
                     /translation="MASLFVIQGADQGKRFEFMSSSVALGRDSSSTVRLHDTEVSRRH
                     AELRLDRNGYRIIDLSSANGTLVNGQTVDQTLLHSGDRVQLGQTVMLFHEGNGGGHRD
                     LTGRVDLLTKSSPEDRSAILNRIPSDEGSRVLKAPDVAAGWLRDRLLNLSVMYRATQA
                     ISDILDIDALSPQILELVFESIGADRGAILLKDESGHLVPKAVRWREGAEPDERMTIS
                     QTIIDYVLEKGEGVITTDAPTDTRFGTAQSIVDFQIREAICVPIQGRHTTLGVLYADI
                     QSSGIGATMAGKSGGRGRFSQDQLMLMVAIGHQAGLAIENTIFYNDKIQAERLAAVGQ
                     TIATLSHHIKNILQGIHGGSSLIDLGLKEKDDTIVRRGWTIVEKNQTKIYNMVMDMLS
                     FSKDREPALEPTDLNETVGDVIELMQSRATELGVTLVWSAGEAIPRVTIDPEGIHRAV
                     LNMITNAIDACEGADNAQVVVKTQWDPRASIARVSVSDNGIGIDEAEIPTIFQIFASS
                     KGSRGTGLGLPVSQKIVREHGGKIIVTSRPGQGATFVIELPMSGRADRGEPPVDGLTM
                     FD"
     gene            complement(2842..3072)
                     /locus_tag="VHS97_10515"
     CDS             complement(2842..3072)
                     /locus_tag="VHS97_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448677.1"
                     /translation="MRSATEAYKRHRQQSLLNLNAGLAESALRHARTCHVLNPGTESN
                     RLMALCYLVQENWNDAVDKAQAVVDKAAKPSL"
     gene            complement(3226..5037)
                     /locus_tag="VHS97_10520"
     CDS             complement(3226..5037)
                     /locus_tag="VHS97_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3448678.1"
                     /translation="MDTDTIIGIDLGTTNSEVAVIRDGRPVVLEEDGEAILPSVVGLD
                     AQGRLLVGKAARNQYVLAPERTVRSIKRKMGQEVTVTLGDQKYAPQEISAIILKTLKH
                     RAEKALGTTISKVVITVPAFFNDGQRQATRQAGELAGMEVVRIINEPTAAVLTYNPHP
                     PEMERLLVYDLGGGTFDVSVAQIENGVVEILASHGDTQLGGDDLDQLLFEHICAGFAR
                     EHGVDIAESVGAKARVLRAAEDAKKRLTFDAVTTVEEEFIAEKNGKPLNLVMEISRYD
                     YEEMIRPLLLKTLTCLDQSLTDAKVNANQIDKVVLVGGATRTPLVHQLLEERLGRPVH
                     CEIEPDLAVAMGAAVQGGLIAGIDVGPILVDITPHTLGIGALGDLHGFLSPHAFSSII
                     ERNTPLPVSRTEIYSTVVDDQDGARIRIFQGEDRDTRYNTLVGEFVIEGLAEVPAGNQ
                     ILVRLELDLNGILKVTATERATGLAKHVVIDNAMERFRLRQRSDALERLDAVFDNLAE
                     DDEPLTALEGLDQNHEPSGALTHDAPVPGVRQATVSAHALIAKAKGLLPAANADDAEE
                     LRAMLTDLEAALDRGVEDDIRAVSHEVEEIVFYLEDA"
     gene            complement(5211..6092)
                     /gene="grpE"
                     /locus_tag="VHS97_10525"
     CDS             complement(5211..6092)
                     /gene="grpE"
                     /locus_tag="VHS97_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX3448679.1"
                     /translation="MNQKSDIDIEATLEKFRHWLSEARASGDGTAALEPNSNSSAHTQ
                     VREFGIIDLIEEFTALRHELKLQTKSGRTLIDQSDAAVSALRQAVERVRAVDVKEAQA
                     VWTAGKALAEALADLDEALLRGEREIERARRLLAAESLSRLESSLEKVYRRRSWLRRR
                     ILRTYHHEVIEAIRSDGAAREQLFDSFLEGYGLIANRLRRVLASEQIAHIPCEGKPAD
                     PELMTVIEVVDEPRDRPGTVVKELRRGYTWRGRLIRCAEVQAVRGAATEVARAHERDA
                     EELEEELDEVGAQLRSI"
     gene            complement(6089..6385)
                     /locus_tag="VHS97_10530"
     CDS             complement(6089..6385)
                     /locus_tag="VHS97_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX3448680.1"
                     /translation="MSNPYEVMGLAAGASEPEIRQRYLELVREFPPDRAPERFAAVHA
                     AYASLRDPALRLYAQLFSIDPGNDSIEGIAADLRARLRDTRLPLDVLLSLAEST"
     gene            complement(6469..>6944)
                     /locus_tag="VHS97_10535"
     CDS             complement(6469..>6944)
                     /locus_tag="VHS97_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448681.1"
                     /translation="ICEFLGHTNQKVATIEKLVAVGLKQYPFSPLLNFRAALLETGKG
                     FFSLGSFRARHFLDRALKLAQASTDPKENALVTPIKKSLTLLNEMEKLPLRPPSVGGG
                     SSGFPFAGFADFFDDFDDDDDGDDDDGDDDDFYGRPASSPRPARGTKKPKGRKRR"
BASE COUNT         1286 a         2108 c         2083 g         1467 t
ORIGIN      
        1 atgggctcga tgatggggat gcgagggatg gcgagtatga tgatgggcgg tgggaccgat
       61 cctcggtctt ccgccaaggc tgcaaatcgc caaatccgtc cgcagatcgc cgccctggcc
      121 gcgggcctgg ctgctcgcga aggcgacccc ctaagtaagg ttgtcctcaa aaagctcgaa
      181 gagcccatct cgatgtcatt cgctaatgaa acgccgcttg atgatgtctt gaagtacatc
      241 aggcaagcca cgacaacccc gacctacgcg ggaatcccga tctacgttga tcccaaggga
      301 ctggaagagg ctaccgccgc gctggattca acggtgagtt ttgacttaga acacgttccg
      361 ctcaagacga cacttcggct gatgctcaag cagatcggcc tggcctactg tgtgcgcgat
      421 ggcgttctga tcattagctc tgttcagggc gtcaacgaag agctcaacga agcacaaagc
      481 gaaatcgacg gaataaagcc tcaaggagga atacagtgaa gccgacgtcg acctcaatcg
      541 aacatcgtga gcccgtccac cggtggttcg ccgcggtcgg cgcgccccga catggggagc
      601 tcgatcacga atgtggcccc ttgcccagga cgagacgtga cgatgatctt accgccgtgt
      661 tcgcgcacga ttttttgtga aaccggcaag cccaaccccg tcccgcgtga ccctttgctg
      721 gacgcgaaga tctgaaagat tgtcgggatc tctgcttcgt cgatgccgat gccgttgtcg
      781 ctgacgctga cgcgggcgat cgaggcgcgg gggtcccatt gcgttttgac gacaacctgg
      841 gcgttgtccg cgccctcgca cgcgtcgatc gcgttggtga tcatgttgag cacggcgcgg
      901 tggattccct cggggtcgat cgtgacccgg ggaatagcct cgccggcgct ccacacgagg
      961 gtcacaccca gctcggtggc gcgcgactgc atgagctcga tcacgtcgcc cacggtctca
     1021 ttcagatcgg tcggctcgag cgcaggctcg cggtctttgg aaaaggaaag catatccatg
     1081 accatgttat agatcttggt ctggtttttc tcgacgattg tccatccgcg gcggacgatc
     1141 gtgtcgtcct tttccttgag cccgagatcg atcagcgagc tgccaccgtg gatgccctgc
     1201 aggatgtttt tgatgtggtg ggagagggtt gcaatcgtct ggccgacggc ggcgagccgc
     1261 tcggcctgaa ttttgtcgtt gtaaaagatc gtgttttcaa ttgccagtcc ggcttgatga
     1321 ccgatcgcga ccatgagcat gagctggtcc tggctgaacc gaccgcgccc acctgacttg
     1381 ccggccatcg tcgcgccgat gccgctggac tggatgtcgg cgtagaggac accgagcgtg
     1441 gtgtgtctcc cctgaatcgg cacgcagatg gcctcgcgga tctgaaagtc gacgatcgac
     1501 tgcgcggtgc cgaagcgcgt gtcggtgggg gcgtcggttg tgatgacgcc ttcccctttt
     1561 tcgagcacat aatcaatgat tgtttgtgat atggtcatcc gttcgtcggg ctcggctcct
     1621 tcgcgccatc gcaccgcctt gggaacgagg tgacctgatt cgtctttcaa gagaatggcg
     1681 cctcggtcgg cgccgatcga ctcgaagacg agctcgagga tctgcggcga tagcgcgtcg
     1741 atatcgagaa tgtcgctgat ggcctgggtc gcgcggtaca tgaccgacag gttcaagagc
     1801 ctgtcgcgca gccagccggc cgccacgtcg ggagccttca agacgcgcga gccttcgtcg
     1861 gatggaatgc gattcaaaat ggccgagcga tcctcgggac tgctcttggt caagaggtcg
     1921 acgcggcccg tcagatcgcg gtgtcctccg ccattcccct cgtgaaacag catgaccgtc
     1981 tggccaagct gaacccgatc gccggagtgc agcagggtct ggtcgaccgt ttggccgtta
     2041 actagagttc catttgccga cgacagatcg atgattcgat agccattgcg gtcgagcctg
     2101 agctcggcat ggcggcgaga aacctcggta tcgtggagcc gaacggtact cgagctgtcc
     2161 ctgcccaggg ccacggaact ggacatgaac tcgaaccgtt taccctggtc ggccccttga
     2221 atcacaaaca aggaggccac gaggctatct ctcctcggct cggttgcgac atcgcgagca
     2281 agtgatgccc gtactatttt caacactaac gcagccggac acgagcgtca acccgtcggt
     2341 tgtgtgtgtg cgtgcggcgc ccagacgatc atccggttcc tgtcgcgttc gctcgcgccg
     2401 cttgagtggc gggcggccgt ggtcgaggct ggcatcagac caattaccgt tgaataagca
     2461 gtaacttaca ggtgcatagc gggttatgat cccctctccc caggcagagg gagccagtaa
     2521 catgcatcgg ccgcgagttt gctgtagcga gcctgtggcc gccctgacag gtgtcagaaa
     2581 aacccctccc tgtgagctcc cacaaagggg aggtcaacgg aaaggatcaa gcactaataa
     2641 gttacaccca tttcaacggc attggcttca agccggcgag cccacggtgc ctgcaactgg
     2701 ggcggcgccc ttcaggcgcc gaccacagcc acccagtctt gccggaaagc gcaaccgaaa
     2761 ccgaattcga tcgcctgttc cagagatctt tccaagacca gcttgtcacc agccaactgt
     2821 acgggcgatc gagcacggac ttcacaatga cggcttcgcc gctttatcca cgacggcctg
     2881 cgccttgtcg accgcgtcat tccagttttc ctgaacgagg taacagagtg ccatcaggcg
     2941 gttcgactcg gtccctgggt tgaggacatg gcacgtacgc gcatggcgca gcgctgattc
     3001 ggcaaggccg gcattcaaat taagcaacga ctgctggcga tgtcgcttgt aagcttcggt
     3061 cgctgaccgc aacaggcgca ggtcgcactt gcatctgcgg cacgtgtcgg cccacacttg
     3121 ccgcgcgccg cacgtcgggc aacgcatttc acgttcagat tcagccggca tattcgctag
     3181 ccaacgcgct ccaattatta ttgacctgaa aggtcaatcc cgcggtcatg cgtcttcgag
     3241 atagaacacg atttcctcga cctcgtggct gactgcccga atgtcgtctt ccacaccgcg
     3301 atctagagcg gcctccaagt cggtaagcat cgcccggagt tcctcggcat catcggcgtt
     3361 ggcagctggc aagaggccct tggcctttgc aatcaaggcg tgggccgaga cggtcgcctg
     3421 gcgaacgccc ggaacggggg cgtcgtgggt caatgcaccc gagggctcgt gattttgatc
     3481 gaggccctct agcgccgtca acggctcgtc gtcttccgcg agattgtcga aaacggcgtc
     3541 gaggcgttca agggcgtcgc tgcgctggcg gaggcggaac cgttccatcg cgttgtcgat
     3601 caccacatgc ttggcgagtc cggtggcccg ttcggtggcc gtcaccttga gtattccatt
     3661 aagatcgagc tcgagccgga ccagaatttg attacccgcg gggacttcgg ccagcccttc
     3721 gatcacgaat tcgcccacca aggtgttgta gcgcgtatcc cggtcttctc cctggaagat
     3781 ccggatgcgc gcgccatctt gatcgtcgac gacagtcgag taaatctcag ttcggctgac
     3841 gggaagcggc gtgttgcgct cgatgatcga cgagaatgcg tggggtgaca aaaacccgtg
     3901 gagatcgcct agagcgccga tgcccagcgt gtgtggtgtg atatccacca ggatcggccc
     3961 gacgtcgatg ccggcgatca ggcccccttg tacggccgcc cccatcgcga cggccaggtc
     4021 gggttcaatt tcgcagtgaa ccgggcggcc cagtcgttct tccaagagct ggtgtacgag
     4081 tggggtccga gttgctcctc ccaccagcac taccttgtcg atctggttcg cattgacctt
     4141 ggcgtcggtg agcgactgat ccaggcacgt gagcgtcttc aagagcaacg ggcggatcat
     4201 ttcttcgtaa tcgtatctgg atatctccat aacaagattg agcggcttgc cgttcttttc
     4261 ggcgataaac tcttcctcga cggtggtcac agcgtcgaag gtcagccgtt tcttggcatc
     4321 ctcggccgcg cgaaggactc gggccttcgc tcccacgctt tccgcgatgt cgacgccatg
     4381 ctcgcgggca aatcccgcgc agatatgttc aaagagcaac tggtcgagat cgtcgccacc
     4441 caactgcgtg tcgccgtggc tggccaggat ctccaccacg ccgttttcga tctgggccac
     4501 ggacacgtcg aacgtgcctc cccccaggtc gtaaacgaga agccgctcca tctcgggagg
     4561 gtgcgggttg tacgtgagca cagccgcggt cggctcattg atgatccgga cgacctccat
     4621 gcccgccagt tcacctgcct ggcgggtggc ttgtcgctgt ccgtcgttga agaaggccgg
     4681 caccgtgatc accaccttcg agatcgtggt gccgagcgcc ttctcagccc gatgcttaag
     4741 cgtcttcaag atgatcgccg agatttcctg gggcgcgtat ttctgatcgc ccagggtgac
     4801 cgtgacctcc tggcccatct ttcgtttgat cgatcggacc gtgcgctcgg gagccagcac
     4861 gtactgattg cgcgcagcct tgcccaccag cagacgacct tgcgcgtcca gtcccacgac
     4921 ggatggcagt attgcctcgc cgtcctcctc gagtaccacg ggacggccgt cgcggatgac
     4981 cgccacttcg ctgttggtcg ttcccaggtc gatgccgatg attgtgtctg tatccatatc
     5041 ctggttcctc cattaagcca gtaccggtcg gatcggtcag ttgaaaccta agtgaaaccg
     5101 caaaactatt tgtgtaggtc aagctttcca gcctgacact ttgtgccctc cagtaagacg
     5161 tcgggctaga aagcccgacc gacgctgcgc gacgactttg gcgggtccac ttagattgat
     5221 cgcaactggg cgccgacttc gtctagttct tcctccaatt cctctgcatc gcgctcgtgc
     5281 gcccgagcga cctctgtcgc ggcccctcgc accgcctgga cttccgcgca gcgaatcaag
     5341 cggcctctcc aggtgtaacc gcgtctcagc tctttgacca cggttccggg ccgatctcgc
     5401 ggttcgtcga cgacttcgat cacagtcatg agttccgggt cggcgggctt gccctcacat
     5461 gggatgtgcg cgatctgctc tgatgcgagc acgcggcgga gacggttggc gatcaaccca
     5521 tacccttcca gaaaagagtc gaacagttgc tctcgcgccg cgccatcgct ccggatggcc
     5581 tcgatgacct catgatggta cgttcgcaaa atacgccgcc tgagccagga tcgccggcgg
     5641 taaacttttt cgaggcttga ctccagccgg ctcaaggact cggcggcaag cagtcgtcgg
     5701 gctcgctcga tttcgcgctc gccgcggagc agagcttcat cgagatcagc gagggcttcg
     5761 gcgagcgcct tccccgcggt ccagactgcc tgtgcctctt tgacgtcgac ggcgcggact
     5821 cgttcgaccg cctggcgcaa cgccgacacc gcggcgtcac tctggtcgat taaggtccgt
     5881 ccgctcttgg tttgcagctt gagctcgtgc cgcagcgccg tgaactcttc gatcagatcg
     5941 atgatcccga attcacgtac ttgtgtgtgc gcgcttgagt ttgagttcgg ttctagagcg
     6001 gccgtaccgt cgccgctggc gcgcgcttcg gaaagccagt ggcggaattt ctcgagggtg
     6061 gcctcgatgt cgatgtcaga cttctggttc atgttgattc cgctagcgaa agcaatacgt
     6121 cgagtggcag ccgcgtgtcg cgcaatctgg cacgcagatc ggccgcgatc ccttcgatcg
     6181 agtcgttccc cgggtcgatc gaaaagagct gggcatagag ccggagggcc gggtcgcgca
     6241 ggctggcgta ggctgcatga acagcggcga aacgctccgg tgcgcggtcg gggggaaact
     6301 cgcggacgag ctcgagataa cgctgccgga tctcgggttc gctggcacca gcagccagcc
     6361 ccatgacttc gtaagggttg ctcacttccc tttaccccct cttgcgccct ttcggtttct
     6421 cggttcgata cgtgcccgac gtcgggctgg aaagcccgac ctaccccttt acctcctctt
     6481 tcgccctttc ggtttcttgg tccctcgggc tggtcgtgga ctcgatgccg gccggccgta
     6541 gaaatcgtcg tcgtcaccgt catcgtcatc accatcgtcg tcgtcatcaa aatcgtcgaa
     6601 gaaatctgcg aaacccgcga acgggaaacc ggacgaaccg ccaccgactg acggaggcct
     6661 gagcggcagt ttttccattt cgttgagtag agtgagcgac ttcttgatgg gggtgaccag
     6721 cgcgttttcc ttgggatcgg tcgatgcttg ggccagtttc aaggccctgt cgaggaaatg
     6781 ccgcgcacgg aagcttccga gcgagaaaaa tccttttccc gtctcgagca gcgcagccct
     6841 gaagttcaac agaggcgaaa aggggtactg cttcaagccg acggcgacca gcttctcgat
     6901 cgtggcgacc ttctggttag tgtggccgag aaactcgcag atgt
//
LOCUS       DATAQL010000603         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2199421, whole genome shotgun sequence.
ACCESSION   DATAQL010000603 DATAQL010000000
VERSION     DATAQL010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199421"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VHS97_10540"
     CDS             <1..1877
                     /locus_tag="VHS97_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX3448682.1"
                     /translation="GQRLRRFFAFLGPAYLVSVGYMDPGNWATDLEGGARFGYALIWV
                     LLMSNVMAVLLQTLAARLGVVTGHDLAQACRAEYSRLLNAVLWVLAEVAIAATDLAEI
                     LGTIIALKLLFGLPLLWGCLITAFDTFLLLYLQRWGIRQMEAVILALVATIGACFLVQ
                     IILARPDMGAVGLGLRPSLPPGALYVAIGILGATVMPHNLYLHSALVQTRGFGADARS
                     KATACRYYMIDAAIALNAAFFVNAAILILSAAVFHRHGMEVSTIEEAYKLLPSFLGRG
                     APILFAIALLCAGQSSTLTGTLAGQIVMEGYLHLRLAPWLRRLVTRLIALVPAVVVIS
                     LAGDQSTQRLLILSQVVLSLQLSFAVIPLIHFTSNRRNMGQFATPWWGQILAWTAAGV
                     IVALNGKLVLDQIGQAVSYAAGLTARFGPIPVSWLVAGALYGVTGGAAMLLGWVTVKP
                     FWRASPAWLPKPSVKLDWVKALRPRRLARIGVALEHDQADAEILNRALGLVQTDGGQS
                     ELVLLHVVDTPITRVLGSETADREAEADERYLADLVAALRDRGYKVRPVLLHGPNPAG
                     ELVSHLRKDPVDLLVVGSHGHGMVRDLLLGQTVDRVRHNLGVPMLIARPDRVHGPPSD
                     "
     gene            2017..2604
                     /locus_tag="VHS97_10545"
     CDS             2017..2604
                     /locus_tag="VHS97_10545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3448683.1"
                     /translation="MRTIGLARAGRVLAMAITYYKRFRMEIDLNGADAMPSLPRGFVW
                     VPWAESLLEQHAEVKYRSFIGEIDAYVFPCLGDRHGCRRLMCEIRRKPGFLARATWLI
                     ACSDGYVATVQGVMDQGPIGAIQNLGVLRKYRGLGLGKALVHRALAGFRQAGLHQAYL
                     EVTAENSAAVQLYRDVGFRRAKTLYKAVDNEVRPY"
BASE COUNT          504 a          868 c          943 g          639 t
ORIGIN      
        1 cggggcagcg gctcaggcgc ttctttgcgt tcctcggtcc ggcttacctg gtcagtgtgg
       61 gttacatgga ccccggtaac tgggcgaccg atctggaggg cggggcgcga ttcggctacg
      121 cgctgatctg ggtccttttg atgtcgaacg tcatggctgt gctgttgcag accctcgccg
      181 cgcgattggg ggtggtgact gggcacgacc tggcgcaagc ttgtcgcgcc gaatactcgc
      241 gcctgctgaa cgccgtcttg tgggttttgg ccgaggtggc catcgccgcg acggatctgg
      301 cggaaattct gggaacgatc atcgcgctca agctgctttt cggtttaccg ctcttgtggg
      361 gttgcttgat caccgcgttc gacaccttct tgcttcttta tctgcaaaga tggggcatcc
      421 gccagatgga ggccgtcatc ctggcgctcg tcgcaaccat cggcgcctgt ttcctggttc
      481 agattatcct ggcgcggccc gatatggggg cggttggcct gggtttgcgg ccaagcctgc
      541 cgcccggggc gttgtatgtc gccatcggaa tcctgggcgc cacggtgatg ccgcacaacc
      601 tgtatttgca ctcggccctg gtccagaccc gcgggttcgg agccgacgcg cgcagcaaag
      661 cgacggcttg ccgctactac atgatcgacg ccgcgattgc cttgaacgcg gcattctttg
      721 tcaacgccgc gatcctgatt ctgagcgcgg cggtgttcca tcgccatggg atggaagtct
      781 caaccattga agaggcgtac aaactgcttc cttcgttcct tgggcggggc gctccgatct
      841 tgttcgccat cgctctgtta tgcgccggcc aaagctcgac cctgaccggg acgctcgctg
      901 gacagatcgt catggagggt tatctccacc tgcggctggc cccgtggctc cgccgactag
      961 ttactcgact gatcgcactg gtcccagccg tcgttgttat ttcgttggcc ggggatcagt
     1021 cgacgcagcg actgctgatc ttgagccagg tggttttgag cctgcaactt tcgtttgccg
     1081 tgattcctct catacacttc acgtcgaacc gaagaaacat gggccagttc gcgacacctt
     1141 ggtggggaca gatcttggcc tggacggcgg ccggggtaat cgtcgcgctc aatggcaagc
     1201 tggtcttgga tcagatcggc caggcggtga gctacgcggc ggggttgacg gcgcggtttg
     1261 gcccgattcc ggtgagctgg ctcgtggcag gtgcgctcta cggcgtgacc ggcggcgcgg
     1321 ccatgctgct gggttgggtg accgttaagc cgttctggcg cgcgtcgcct gcctggctgc
     1381 ctaaacccag cgtgaagctc gactgggtaa aggcgcttcg gccgcgccgg ctcgcacgaa
     1441 tcggggtcgc gctcgaacac gaccaggccg acgcagagat cttgaaccgt gcgctggggc
     1501 tggtccagac cgacggagga cagagcgagc tggtcttgct tcacgtcgtc gacactccga
     1561 tcacgcgcgt tctgggctcg gaaacagccg accgcgaagc cgaagctgac gagcgttacc
     1621 tagcggatct ggttgcggcc ctgcgcgacc gcggatacaa ggtccgaccg gtcttgttac
     1681 acggccccaa cccggccggc gaattagtca gccacttacg gaaagacccg gtggaccttc
     1741 tagtcgttgg ctcgcatggc catggaatgg tccgcgacct gctcctgggc caaactgtcg
     1801 atcgagttcg ccacaattta ggcgtgccga tgctcatcgc tcgcccggac cgcgtgcatg
     1861 gcccgccctc ggactaaaag gggtcaggac gctgtggtgc gttaaagggg cctgaccctt
     1921 ttttcgaggg ccctgccggg tctcgctgag tcaagaattg accctcgatc ccgcgtgacc
     1981 ttataatgca aaagtataga aaaagagagc ggtctcttgc gaacaatcgg actggcgcgt
     2041 gcgggtcgtg tcctcgcgat ggccatcacg tattacaagc ggttccgcat ggagatcgac
     2101 ctgaatggcg cggacgcgat gccgtctttg ccgcgcggat tcgtctgggt gccgtgggca
     2161 gagtcgctct tggagcaaca cgccgaggta aaatatcgca gttttatcgg cgaaatcgat
     2221 gcgtatgtgt tcccctgtct gggggatcgg cacgggtgcc ggcgcctgat gtgcgaaatc
     2281 cgccgcaagc ctgggttcct tgcccgcgcg acctggttga tcgcgtgctc cgacggttat
     2341 gtcgcaacgg tccagggtgt gatggatcag ggcccgatcg gggcgattca gaatttgggt
     2401 gtcctccgca aataccgggg actcgggctc ggcaaggcgc tagttcaccg cgcgctcgcc
     2461 ggctttcgcc aggcgggatt gcaccaggcc tatctggaag tgaccgctga aaactccgcc
     2521 gcagtccagt tgtatcgcga cgtgggattt cgaagggcca agacgctcta taaagccgtc
     2581 gataacgagg tgagacccta ttgataatcg cgtcttttgg ctattgcttc ccctaagaaa
     2641 gcgatatcga acgcgcgaac ctctccgaaa atggcgacga tacttgatca ctcttcaaca
     2701 tgcagggaag attctggtag gctctcacgt tcaatgacag ggattcggac cgtcctacgg
     2761 tcgaagcagg tcgcgacgac ttctggccgc ttgccacgga tggcccgtgc aatgacggcg
     2821 ggatgtccat gaaaacagtt cgaggtcccc ttggccgcag cattgtcttc catgctggaa
     2881 caacacacct ttcccaacgg gctggtgctg gtcgccgaga acatgccggg cgtccagtcg
     2941 gcggcgttca catt
//
LOCUS       DATAQL010000604         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1742159, whole genome shotgun sequence.
ACCESSION   DATAQL010000604 DATAQL010000000
VERSION     DATAQL010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742159"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VHS97_10550"
     CDS             <1..215
                     /locus_tag="VHS97_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX3448684.1"
                     /translation="IGASGAVEAAATALSLYRGVVPPTINLATPDPDCDLDYVPNTSR
                     EMPLETAVSNSFGFGGQNASLVMTRM"
     gene            509..919
                     /locus_tag="VHS97_10555"
     CDS             509..919
                     /locus_tag="VHS97_10555"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX3448685.1"
                     /translation="MSKPGPGAGDRRRVCRHPVVYQDASLGWWLESEFKSTPVRLVDL
                     SLNGCLIELARAPRLGEQQSIWLHAHSLVVAEWTEGRIVSIKKTWAGKCRLGIAFLAP
                     MAYEPFKKLIYGREHFQESPPDDLPEHERDHFWK"
     gene            1023..2384
                     /locus_tag="VHS97_10560"
     CDS             1023..2384
                     /locus_tag="VHS97_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3448686.1"
                     /translation="MLERRLKLLEAVSLNMSMMVGIGPFITIPALVATMGGPQSMIGW
                     VLGAAVALFDGMVWSELASAFPGSGGTYHFYDAAYGNSRSGRLLKFLFVWQFLFSGPL
                     EVATGAIGLAKYLGYFYPILAEPAWTWDALFPAFPGEVAWGQLAAMGVMAAVTCLAYR
                     RVAVAGRLMVALWIGMLITVAWVIVTGVTHFDATLAFDFPDWTWGKGGLTRAGLGMAL
                     AIAMYDFLGYYQICYLGEEVANPSRTIPRSILISVAVIAVVYLMMNIGILGVLPWREV
                     VSSKHIASDLMLRVHGPRAAALATAMIIWTALASTYAALLGYSRIPYASARAGHFFKA
                     FAQTHPTAHFPHRSLLLISGIATLACLANLETVIQALLTSRILIQFVGQIVTVFYLRK
                     KAKGPASTYRMPLYPLPALLALSGWLFVFSTSEPVVLAYGLLSLLAGAAAFFFWNKAV
                     ATV"
     gene            2467..2751
                     /locus_tag="VHS97_10565"
     CDS             2467..2751
                     /locus_tag="VHS97_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HEX3448687.1"
                     /translation="MGGRDAARASFFRLGQRRALSQFVAEVMMSLDKSLKKAGSLARA
                     RNVLSRAERLALLQEDELWKPAAGVYNLPKTKYRRLAPGQSGPKRPEVTS"
     gene            complement(2783..>2989)
                     /locus_tag="VHS97_10570"
     CDS             complement(2783..>2989)
                     /locus_tag="VHS97_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX3448688.1"
                     /translation="RTWQFPSFRRAIEFTNRVASIMEKTDHYPDLIVNYRTVRIEMST
                     HDVGGLSERDFSLIAEINEIPTDR"
BASE COUNT          536 a          873 c          886 g          694 t
ORIGIN      
        1 tgattggcgc ctccggcgcc gtcgaagcgg cggcgaccgc gctttcattg tatcgcggcg
       61 ttgtccctcc gaccatcaac ctcgcaacgc ccgaccctga ctgcgatctc gactatgtgc
      121 ccaacacgtc acgggagatg ccgcttgaaa ccgctgtgtc caatagcttt ggcttcggcg
      181 gccagaacgc ttcgctcgtc atgacccgaa tgtgattttg aagctgtcca aaagaccagt
      241 caattgcgcc accgcgacca ggcgtctcac ttcgggagtt taccggccga cctattccct
      301 ggttgcccat aaattaatga ctacagactt gtttgtcttt ttgacagcgg cccgaccacg
      361 aactaggatt tcttatcgaa ggcgatcgtc atcgcttccg cgcatggatg ggccagttag
      421 cgagcggttt gcaatttacc aaggatggaa atgatgcgtg cgcggcgccg ttcacacaat
      481 gaatccctcg aatttcaagg tgagagacgt gagtaagccg ggtcctggag ccggcgaccg
      541 ccgccgggtt tgccgccatc ctgtcgtcta ccaggacgcc tctctggggt ggtggctcga
      601 gtctgaattc aagagcacgc ccgtccggtt agtcgacctt tccttgaacg gatgcttgat
      661 cgagctggcg cgcgccccgc gtctcggtga acaacaatca atctggttgc atgcacactc
      721 gcttgtggta gccgaatgga ccgaagggcg aatcgtctcg atcaagaaaa cctgggccgg
      781 taagtgccgt cttgggatcg cattccttgc cccgatggcg tacgagccct tcaagaaact
      841 gatttacggg cgggaacatt tccaggagtc tccaccagac gacttgcccg agcatgaacg
      901 ggatcatttc tggaagtgaa gcgcagccgc cacgactcga ctggcgaccg ggtctgcgtc
      961 gattatgata tcgggtcgcc cgatggatgg ctcactcggg cgcaagaccc ggggtcccgt
     1021 tgatgctgga gcgtcggctc aaactgctcg aggccgtcag ccttaacatg tcgatgatgg
     1081 tcggaatcgg tccgttcatc acgatccccg ccttggtcgc gacgatgggt ggtcctcagt
     1141 cgatgatcgg ctgggtcttg ggcgcggctg tggctctctt cgatgggatg gtctggagcg
     1201 agcttgcctc cgcgtttccc ggctcggggg gaacgtacca cttctatgac gcggcgtacg
     1261 gcaattcccg atcgggccgc ctgctcaaat ttctctttgt ctggcaattt ttgttcagcg
     1321 gtcccctcga agtggcgact ggagcgatcg gcctggcaaa gtatctcggc tatttctacc
     1381 caatattggc cgagccggcc tggacctggg atgcgctctt tcccgcgttc cctggggagg
     1441 tagcctgggg gcaactagcg gcaatggggg tcatggcggc ggtcacgtgc cttgcgtatc
     1501 gccgcgtcgc cgtcgccggt cggctgatgg ttgccctgtg gatcggcatg ctgatcacgg
     1561 tggcttgggt catcgtgacg ggagtgaccc atttcgacgc aacacttgcc tttgattttc
     1621 cagattggac ttggggaaag gggggactga ccagggcggg gttgggcatg gccctcgcca
     1681 tcgcgatgta cgactttcta gggtactacc agatttgcta tctcggcgag gaagtcgcca
     1741 acccgtcgcg gaccattccc cgatcaatct tgatttcggt ggctgtgatc gccgtcgtgt
     1801 atctgatgat gaatattgga attctgggcg tcctgccctg gcgcgaggtc gtctcgtcca
     1861 agcacattgc cagcgacctg atgctacgcg tccatggacc acgggcggcg gccttggcga
     1921 cggcgatgat tatctggacc gcactggctt cgacctatgc cgcgctcttg ggctacagtc
     1981 gcattcccta cgcgtcggcg cgggccggtc attttttcaa agcgtttgcc cagactcatc
     2041 cgacggccca tttcccccac cgctcactcc tcttgatcag cggaatcgca acgcttgctt
     2101 gcctcgccaa cctcgaaacg gtaattcagg cgctacttac gtcgcgcatc ttgatccagt
     2161 ttgtcggcca gattgtgact gtcttttacc tgagaaaaaa ggcaaagggc ccggcgtcca
     2221 cgtaccgcat gcccctttat cccctgcccg cgcttttagc gctctcaggc tggctgtttg
     2281 tcttctccac gtcggaaccg gtcgtgctgg catatggcct tttgtcgttg ctcgcaggtg
     2341 cggcagcctt cttcttttgg aacaaggcag tggccaccgt ctgaatcgct cgccttcaag
     2401 cgatcatggt gcgcttgatt ccgcgggttt ctgcgagtac catcaaggac taagtattcg
     2461 atcaggatgg gagggcgtga cgccgcccga gcgagtttct tcaggctcgg ccagcgccgc
     2521 gccctttctc agtttgtcgc cgaggtgatg atgtcactgg ataaaagttt gaaaaaagcg
     2581 ggcagcctgg ctcgggcacg caacgtcctg tcgcgggcgg agcgactggc acttttgcaa
     2641 gaggatgaac tctggaagcc ggccgccggc gtttacaacc tgcccaagac caagtaccgc
     2701 agactggcac ctggtcagtc cggacccaag cgaccggaag tcacctcctg attctggcgt
     2761 tgcgctcggg ccgctctggt attcagcggt cggtcggaat ctcgttgatt tcggcaatca
     2821 gcgagaagtc gcgttcgctc agaccaccga cgtcatgggt ggacatctcg atgcggaccg
     2881 tccgatagtt tacgattaga tcggggtaat ggtccgtctt ttccataata ctcgcaacgc
     2941 gattggtgaa ttcgatcgcg cgccggaatg aagggaattg ccacgtccg
//
LOCUS       DATAQL010000605         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4641767, whole genome shotgun sequence.
ACCESSION   DATAQL010000605 DATAQL010000000
VERSION     DATAQL010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4641767"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..429)
                     /locus_tag="VHS97_10575"
     CDS             complement(271..429)
                     /locus_tag="VHS97_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448689.1"
                     /translation="MSDFWYNGGSSYTIVGGYSNQSVNNMNDQNMPIGQAYMVDYNSA
                     TGQFSHWT"
     gene            868..1623
                     /gene="hisA"
                     /locus_tag="VHS97_10580"
     CDS             868..1623
                     /gene="hisA"
                     /locus_tag="VHS97_10580"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX3448690.1"
                     /translation="MKTMQVIPAIDLRGGHCVRLRQGDFDQETVFGDDPAAMAAHWES
                     EGAARIHLVDLDGAKAGRPVNVEAVREILKQVNVPCQLGGGIRDQDTIATWLDAGLDH
                     VVVGTQALRDPDWFGQMAETYPGRLILGLDALDGRVATQGWLDVTSVQALTLARQFDS
                     LALTAIIYTDIARDGTLEGPNVPAITALANAVRTPVIASGGVGDLQDLDRLAVLPIAG
                     CIVGRALYDGRFSLADAIGRSSAGRSANLVEPE"
     gene            1724..3802
                     /gene="fusA"
                     /locus_tag="VHS97_10585"
     CDS             1724..3802
                     /gene="fusA"
                     /locus_tag="VHS97_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX3448691.1"
                     /translation="MPTTYHISDIRNVALAGHGASGKTSLADALLFASGATSRKGSVD
                     DGTSTLDIDEEEKRRHFTIDCHLGHLAWTGKQVHLIDTPGYPDFIGNALSALAAVENV
                     VVAVSGPSGVEVNTRRLFQEAGRLGLGRFVVITKMDAENVDYRSDLAAIRETFGTLCV
                     PFNVPVGQGSTFSGVVDVIQSHDEDPPLCPLAPTEAYRMVVEQIVELDEDLMMRYLDG
                     ETVEPDELKKAAHDAIAQGKLVPVLCVCTRKDLGLRELLDLISTCGASPADIHRFGTR
                     GGDDDASEEEILPAEDGTLVAQVFKTVNDQFMGKLAYVRILTGRLSSDTTIVNLRSGK
                     SAKAGHIYVLQGKQQEEVPEAIAGDIVAIAKFDDLHVSDTISNVGGNTTVPQLRARPI
                     KFPMPMVPRAVEPKARDDEAKISAGLAKIADEDPTFAIRRDAKTHELVISGMSDLHLD
                     VIQQRLKNRHKVEMSTHVPHVPYLETITSDADANHRHKKQTGGRGQFGEVHLRIRPRE
                     RSQGFNFVNAVKGATIPNQFIPAVEKGVREQMDKGVISGNQVVDVEVEVYFGKDHPVD
                     SSEQAFKTAGATAFRKAFEQAYPVLLEPIVSIEITVPGEKFGDISADLSTRRGHITGM
                     DSISGNMQIIRAEVPLAEVLTYASQLKSMTAGQGSFTMDFKSYDPVPPNVQAHIVDKY
                     KKSRAGIEEE"
     gene            complement(3912..>4344)
                     /locus_tag="VHS97_10590"
     CDS             complement(3912..>4344)
                     /locus_tag="VHS97_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX3448692.1"
                     /translation="IRESIVIGVVASVISNLAVHWKTRSTLDDTLDVFPCHGVGGITG
                     MIMTGIFAKDVGLTSGKYETFFYHLLALVIVAVFAFGGSWLLYKITDLVIPLRVTYDQ
                     ETLGLDLSQHGESALGSDLLGGVHANGNGAIKQSELAANLS"
BASE COUNT          917 a         1294 c         1310 g          823 t
ORIGIN      
        1 gaaataataa gttgagcgta tttcggcacc cgtagtggca cgggcttcca gcccgtgtct
       61 gaaaatacca ctagtggcac gggcttccag cccgtgtctg aaaataccac gggcaagatg
      121 cccgtgccac ttaagccagc cgcgcctact tgctcaactt atactttcgc ggctgcttag
      181 ggtagcagat cctgcacaac ccagccaagc ttctgcgagc cggcgctcca ctcgttgtga
      241 cgtcatggga cgtgagccca gcagacgatt ttacgtccag tgagagaatt ggcctgtggc
      301 cgaattataa tcgaccatgt acgcttggcc gatcggcatg ttttggtcgt tcatattgtt
      361 gacggattga ttgctatatc cgccgacgat ggtgtagctc gagccaccgt tgtaccaaaa
      421 atctgacata cccgggattg ccggggtgtg cgctgaaacc aagttcggcg accacgttcg
      481 ggtccgccga acacggcctg ggattgagcc gactgtccct gtccagggaa cagccagagc
      541 tggaattccc cgaatcctct tcgattgcct cgtccggtga gtcgggatga ccagcgcagc
      601 tcttcttccc tctcaacgcg tagaacagga aatcacggaa agcaaaaacc gggacaggga
      661 aaaacggagc agcattgcgg gggcgactcg tccacgctgt ggcgaatcca gccagtcacg
      721 attggaataa gtcattgacg acgtgaagca gttggtgaat tccttgggac ggaaacgatg
      781 agacattgaa accctcgttg ggtcggtcct tgcgacactc cggcgacgga gatagattga
      841 acctattgaa ggatctctgt ggaattggtg aagacgatgc aagtgatccc ggcgatcgac
      901 ctgcggggag ggcactgcgt ccggctccgg caaggggatt tcgaccagga aacagtattt
      961 ggtgacgatc ccgcagcgat ggctgctcac tgggaatcgg agggggcggc gcgcatccac
     1021 ttggtcgatc tcgacggtgc caaggccggc cgtccggtca atgtcgaggc ggtcagagag
     1081 atcctcaagc aggtaaacgt tccgtgccag ctcggcggcg ggatacgcga ccaggacacg
     1141 attgccacct ggctggacgc ggggttggac catgttgtcg ttgggacgca agccctgcgc
     1201 gacccggatt ggttcggcca gatggcagag acctatccgg gccggctgat tctggggctc
     1261 gacgcactcg acgggcgggt cgcaacccag ggctggctcg atgtgacttc tgttcaagca
     1321 ctcacgttag cccggcagtt tgactcactg gcgctcaccg ccatcattta caccgatatc
     1381 gcacgcgatg gcacgcttga gggccccaac gtgccggcca tcaccgcttt agcgaatgcc
     1441 gtccggacac cggtgatcgc ctcgggaggg gtcggagatt tgcaagatct ggatcggctg
     1501 gcggtgcttc ccatcgcggg ctgcatcgtg ggccgagcct tgtacgacgg ccgattctcg
     1561 ctggctgacg ccatcgggcg gagctccgcg ggccgttcgg ccaatctggt ggaaccggag
     1621 tagtagtctc gattgcacgt ctgggctccc agctcattct ggcaaaggga gcggctcgaa
     1681 acccgatcag ttcaaccttt ccgatacagg gagaatcgac gccatgccca caacctatca
     1741 cattagcgat atcaggaacg tcgccctcgc tggacatggg gcgtcgggca agacaagtct
     1801 tgccgacgcc ctgctttttg cctccggcgc gactagccgc aagggctctg tcgatgacgg
     1861 caccagcact ctcgacatcg acgaagaaga gaaacgccgg cacttcacga tcgattgcca
     1921 cctggggcat ctcgcatgga ccggcaagca agtccacctg atcgacaccc cgggctatcc
     1981 cgactttatc ggcaacgcac tcagtgcttt ggccgccgtc gaaaatgtcg tcgtcgcagt
     2041 ttcggggccc tcgggggtcg aggtcaacac gcgcaggctg ttccaggaag caggccggct
     2101 gggcctgggg cggtttgtgg tgatcaccaa gatggatgcc gagaacgtcg attatcggtc
     2161 cgacctggcg gcgatccggg aaaccttcgg cacattgtgc gtgccgttta acgtgcctgt
     2221 aggacagggt tcgacgtttt cgggcgtggt cgatgtgatc cagtcgcacg acgaagaccc
     2281 tcccctttgt ccgctcgcgc ccaccgaggc gtatcgcatg gtcgtcgaac aaatcgtcga
     2341 gctcgacgaa gacctgatga tgcggtactt ggacggtgaa accgtcgagc cggacgagct
     2401 gaagaaggcc gcccacgacg cgatcgccca gggaaagctc gtgcccgtgc tctgcgtgtg
     2461 cactcgcaag gacctcggcc tccgcgagct actcgatctg atctccacct gcggcgcgag
     2521 ccccgctgac atccaccgct ttggtacccg aggcggcgac gacgatgctt ccgaagaaga
     2581 aatcctgccc gccgaagacg gtacgttggt tgcccaggtc ttcaagacgg tcaacgatca
     2641 gttcatgggc aaactcgcgt atgtacgcat tctgaccggc cggctgtcgt ccgacacaac
     2701 gattgtcaac ctccgcagcg gcaaatcggc caaagccggc cacatctacg tgctccaggg
     2761 caagcaacaa gaagaggtac cggaggcgat tgccggcgac atcgtggcaa tcgccaagtt
     2821 tgacgacctg cacgtttccg acacgatcag caacgtcggc ggcaacacga cggtgcccca
     2881 gcttcgggcc cggccgatca agttcccgat gccgatggtg ccccgcgcag tcgagcccaa
     2941 ggcgcgcgac gacgaagcca agatctcggc cgggctggcc aagatcgccg acgaagaccc
     3001 cactttcgcg atccgccgtg atgccaagac gcacgagctc gtgatctcgg ggatgagcga
     3061 cctgcacctg gacgtcattc agcagcgcct gaaaaaccgc cacaaggttg agatgagtac
     3121 ccacgtgcct cacgtgcctt atctcgaaac aatcaccagc gatgccgacg ccaaccatcg
     3181 ccacaagaaa caaaccggtg gacgcggcca gtttggcgag gtccacttga ggattcggcc
     3241 gcgcgagcgg agtcagggct ttaacttcgt caacgcggtc aaaggtgcta ccattcccaa
     3301 ccagttcatc ccggccgtcg aaaagggcgt tcgcgagcag atggataaag gggtgatctc
     3361 gggcaatcaa gtcgtggacg tcgaagtgga ggtctacttc ggcaaagacc atccggtcga
     3421 cagctcagag caggcattca agaccgccgg cgcgacggcc ttccgcaagg ccttcgaaca
     3481 ggcgtatcct gtgcttctgg agccaatcgt ctcgatcgag atcacggttc ccggcgaaaa
     3541 attcggcgat atctcggccg atctttccac ccgccgcggt catatcacag gcatggattc
     3601 catatcgggc aatatgcaga tcatccgcgc tgaagtgccg ctggccgagg tgctcaccta
     3661 cgcgtcacag ctcaagagca tgactgccgg tcagggctcg ttcacgatgg atttcaagtc
     3721 gtacgacccc gtgccgccga acgtccaggc acatatcgtc gacaagtaca aaaagtcgcg
     3781 cgcggggatc gaggaagagt gaaaggctcg cgtttgcccg aacgccgaac accttgtgtc
     3841 tgattcggcc gggatcaccc cccctgatcc cccccttcac aaggggggga aatttgattt
     3901 gattgcggtt tttatgagag attggcggcc aattctgact gcttgatggc tccattgccg
     3961 ttggcgtgga cgccgcccaa gaggtcggag cccagggccg attcgccgtg ctggctgaga
     4021 tcgaggccca gggtttcctg gtcgtaagtc acgcgcagcg ggatgacaag gtccgtgatc
     4081 ttgtagagca accacgagcc tccgaacgca aacacggcca caatcacgag ggccaggagg
     4141 tgatagaaaa acgtctcata ctttcccgaa gtcagtccca cgtccttggc gaaaatgccg
     4201 gtcatgatca tcccggtgat gccgccgacg ccgtggcacg ggaaaacgtc gagcgtatcg
     4261 tcgagcgtcg agcgcgtctt ccagtggact gccaggttgc tgatgacgct ggcgacgacg
     4321 ccgatcacaa tcgactcgcg gatt
//
LOCUS       DATAQL010000606         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3574945, whole genome shotgun sequence.
ACCESSION   DATAQL010000606 DATAQL010000000
VERSION     DATAQL010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574945"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..843
                     /locus_tag="VHS97_10595"
     CDS             91..843
                     /locus_tag="VHS97_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX3448693.1"
                     /translation="MILDQINYEQLLIPLNKFYDLYIGGSRRPVFFEIAATRPELLDL
                     DRNYHVIREELLGVLPETRAIPRYHELDEMQYNISARVDPDKDWKIYPLNIMGVKPEA
                     YCARCPQTMALLEGIPGLFEAFFSILQGGKSIPHHEGPYRGYLRYHLGLLVPEKNPPS
                     IRLKDQVYTWKEGESVLFDDSWDHEVYNHCEADRVVLIVDIRRPMPQPFDGVNRIVQR
                     IMKAVYGKHVLERLAAMTPPKSPESAAVPLKA"
     gene            complement(1585..1986)
                     /locus_tag="VHS97_10600"
     CDS             complement(1585..1986)
                     /locus_tag="VHS97_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448694.1"
                     /translation="MDLSNANGTASADDQIIVGVISNQLQHFRNIDPPCRGMELPDGV
                     LLRHTRSRSLYFYLKTSIENGKIKTQIHASDSPYDRQKARIGTVITAMFEARADAEHL
                     EKLEKVIRDWVEFVQESLDTVRDFHTFNLET"
     gene            complement(2058..>2947)
                     /locus_tag="VHS97_10605"
     CDS             complement(2058..>2947)
                     /locus_tag="VHS97_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448695.1"
                     /translation="LVLRDEDALLAGLAALSNRSLFVFGPPGNGKSSLSRMLHGALHG
                     ELWVPHCIGIEESVIRLFDPQVHERVEAATDQPRSIDQRWVRIRRPLVVGGSEMTLDS
                     FDLNYSRALRFYEAPLHLKANGGTFLIDDYGRQRIDPRELLNRWIIPLEHRIDYLTLE
                     TGQKIQVPLLQILIIATNLELGAVTDPAFLRRMGYRLFLGPPTPEQYAQIFERYAEQH
                     AAPVAPDLVGRLLDRYQAEKRELRCCEPRDLVERARDICRFTGRPLELNDDVMALAWT
                     GYFGKKSEIHGIQISENGQ"
BASE COUNT          629 a          876 c          869 g          573 t
ORIGIN      
        1 accgcggcgt tctgtgactt ccaccattcc ccaaggaggc ctgatccatc cggccgacgg
       61 agcggacgac aaccagggag aagcagagcc atgatactcg atcagatcaa ttatgagcag
      121 ttgctcatcc ctctcaataa gttttacgat ctgtacattg gcggttcgcg ccgaccggtc
      181 tttttcgaga tcgctgcaac ccgccccgag ctgctggatc tcgaccgtaa ctatcatgtg
      241 atccgcgaag agctgctggg ggtcctgccg gagacacggg ccatccctcg gtaccacgag
      301 ctggacgaaa tgcagtacaa catttcggcg cgggtcgacc cagacaagga ttggaagatc
      361 tacccgctca acatcatggg ggtgaagccc gaggcgtatt gtgctcgctg cccgcagacg
      421 atggcgctgc tggaaggcat tcccgggtta ttcgaggctt tcttctcaat cctgcaaggg
      481 ggaaagtcga tcccacacca cgaagggccg taccgcggct acttgcgcta tcacctcggc
      541 ctgctcgtac cggagaaaaa ccctccatcg atccggctca aagaccaggt ctacacttgg
      601 aaggaaggcg agagtgtact gttcgacgac agttgggacc atgaggtcta caaccactgc
      661 gaggcggacc gagtggttct gatcgtcgac attcgccgcc cgatgccgca accgtttgac
      721 ggcgtgaacc gaatcgtgca acggatcatg aaggcagttt acggaaaaca cgttctcgag
      781 cggctcgctg ccatgacgcc gccaaagtcg ccagagtccg ccgccgtgcc tttgaaggcc
      841 taatctcgat cgcgccggga gcggagcggt gaccgcgtca cgcaattcca tcggaccgag
      901 tcgggccgcc cccgagtcta aaccgattcc agaacaaagg accgcgacat gaccgacaaa
      961 gacccacacg gtcccgcgga cgcaaaactg acgagtaagc tgctcaaaac gcccaggcca
     1021 attgaccatg ataccacaac acgtcaacgg ccacatgagt gatggcgcgt ggccccgcga
     1081 cttcttcatt ctccgcttaa ggttcgacgg cagttgctga ctgaggcatg ttgccgctcg
     1141 aaaaaaacca tcggagcgat ggttcctgca agcgatacgc tcaaaagtcc cggaacacgt
     1201 tgatatcgga tatcaaaggc ttgcgacctg cgaaccgcga ggcgatcgca gcgtacgcgg
     1261 gaagctcctg cgagtgccga acaggagttt cagaagccag gatcaccaat gcgcgcgttc
     1321 gtcccatgcg aactccgaac cctcttgcgc gacacatcgt gccggccatg ccttttgatc
     1381 gcgtactgcc ccgtgtgccg cgtgccatcg gaaagcagac gcgcggctcg ataacgcggc
     1441 ttctacctgt ggactttgcc gacaaacata gagcggccga cacccccagc gacagtcgtg
     1501 ggatccctga ccgactgcgg cgcataagat cggccccacg cgcgagcatg cggtggcggc
     1561 cagcgagaag cccgctgatc aaggtcaagt ttcgagattg aatgtgtgga aatcgcgcac
     1621 cgtgtcgagg ctttcttgga cgaattcaac ccaatcgcga atcacctttt ctaatttttc
     1681 gaggtgttcg gcatccgccc gtgcctcgaa catggccgtg atgactgtgc cgatgcgggc
     1741 cttctggcgg tcgtagggag aatcgctggc atggatttgg gtcttgatct tgccattctc
     1801 gatgctggtc ttgagataga agtacagcga gcgcgaacgg gtatgacgga ggagcacgcc
     1861 gtccggcagc tccatgcccc ggcagggagg atcgatgttc cggaagtgct gtagctgatt
     1921 actgatcacg cccacgatga tctggtcgtc agcggaggcc gttccattgg cgttgcttaa
     1981 atccatcctg ggcgtccccc atttccgagc gtgttggtcg agcacaggtt ttattaaagc
     2041 ttagcaccgg aacgatctca ctgcccattt tcggaaattt ggatgccgtg gatctctgat
     2101 ttcttgccga aatagccggt ccacgccaat gccatgacgt cgtcgttgag ctccaggggc
     2161 cggcctgtga aacgacagat gtcccgcgcg cgttcgacca ggtcgcgcgg ttcgcagcaa
     2221 cgaagctctc gcttctcggc ttggtaacga tcgagcaacc gtccgacgag atcaggtgcg
     2281 accggtgctg cgtgttgctc ggcgtaccgc tcgaagattt gggcgtactg ttccggcgtc
     2341 ggtggaccca ggaagaggcg atagcccatg cggcgcaaga aggccgggtc ggtcacggct
     2401 cccaactcga ggtttgtggc gatgatcaga atctgaagca aggggacctg aatcttctgg
     2461 ccggtctcta gggtcaggta gtcgatacgg tgctcaagcg ggatgatcca gcgattcaag
     2521 agctcacggg gatcgatcct ctgccggccg tagtcgtcga tcaaaaacgt cccgccattg
     2581 gccttcaagt gcagaggtgc ctcgtaaaac cgcagcgcac ggctgtagtt caagtcgaac
     2641 gagtcaagcg tcatctcact gccgccgacg accaggggcc ggcggattcg cacccagcgc
     2701 tgatcgatcg accttggttg gtcggtggcc gcttcgaccc gctcgtgcac ctgcggatcg
     2761 aaaagtcgaa tgacgctctc ttcgatgccg atgcaatgag gcacccagag ctcgccatgg
     2821 agcgcgccgt gcaacattcg gctcaggctg gacttgccgt taccgggcgg cccaaacaca
     2881 aacaagctgc ggttcgataa ggcagccagg ccagccaaga gggcgtcttc gtcgcgcagc
     2941 accaggc
//
LOCUS       DATAQL010000607         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4184754, whole genome shotgun sequence.
ACCESSION   DATAQL010000607 DATAQL010000000
VERSION     DATAQL010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4184754"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VHS97_10610"
     CDS             <1..456
                     /locus_tag="VHS97_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448696.1"
                     /translation="IFQTVGAGLLYVPINTAAYAYLAKEQTNSASGLFNLIRNEGSSI
                     GVSLTNTLLARRTQFHHSRLVEGLDRYNPVAMQWLDGAAGTVRAVGSDPALAARQAVR
                     MLDGAVNQQASMMSYLDVFWLFAMLSFAVIPFIFMMKKAVSRGGGLAAH"
     gene            628..2010
                     /locus_tag="VHS97_10615"
     CDS             628..2010
                     /locus_tag="VHS97_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3448697.1"
                     /translation="MTYLVRRRFAMVLAIALLWPEARGEDLNDAWSQAIGLNAGLQAR
                     EFDSFAAGMNLGAARSARLPSVSLTSLNAFLSQSPVIRVPASLAGSGALASKGGFPAI
                     GQNQTDIPFVFANINQPIYQGGRIRGNIEASKAGVNYQKTEEFRTVLDLKLTVAEAYV
                     GVLRARRNLGVLRSNVAQLSSFLRDVTNRKNEGRATRNEELAAEVSLANAKLQEISAR
                     KDLDAAWATYNRYLYRPPSRVVDLQEIIGPPSSASLDSLTAHAINEQDSSFTLNEPMA
                     ADLTGRALETRPELQGLAERARQSSALANATMATIKPQVSFNASYIDLGLQNFTNPNI
                     FAGTFQLSWTLFDGGQTRRRAEAQRLQGRSYLKQRADASADVVLQVRTRMLDVDESRQ
                     RVIVSGAAVASANENIKVVTERYRQGLSIYTEVLDAENRRIQSLNGYFAAVYDNALAR
                     FRLHRAAGDL"
     gene            complement(2182..>2881)
                     /locus_tag="VHS97_10620"
     CDS             complement(2182..>2881)
                     /locus_tag="VHS97_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3448698.1"
                     /translation="LDEIGDVSPSLQVKLLRTIEHREVTPVGDARPRFTDIRVIAATN
                     RPLGALMAAGEFREDLFFRLSVFQIHLPPLRERREDIPSLADHFLRQVPLANVAAMPL
                     TGDVLDELRNRAWTGNVRELRNAIEHAAIVARGQPIRPEHLPTATARPDLIAPSNTIE
                     IQGLLALWARRAFRDQDGSGDATVYEQFLQLAEPPVLRALLEECGHNRAAAAQILGIH
                     RATLRQKLLKYGIE"
BASE COUNT          548 a          855 c          915 g          563 t
ORIGIN      
        1 atctttcaaa cggtgggggc cggcctgctc tatgtaccca tcaatacggc cgcgtatgcc
       61 tatttggcaa aagagcagac caatagcgcg tcggggttgt tcaacctcat ccgcaacgag
      121 gggtcgagca tcggcgtatc tctgacgaat acgttgctgg cacgacggac tcaattccat
      181 cacagccgat tggtcgaagg gctcgaccgc tataacccgg tggccatgca gtggctcgat
      241 ggggcggctg gcacggtgcg ggccgtcggc tccgaccccg cgcttgcggc ccgccaggcg
      301 gtgcggatgc tcgacggcgc ggtcaaccag caggcgtcca tgatgtcgta cctcgacgta
      361 ttctggctgt ttgcgatgct ctcgttcgcc gtgatcccct tcatcttcat gatgaagaag
      421 gcggtctcgc gcggcggagg gcttgccgcc cactgatctc tgtatcctct acgatcgccg
      481 cgatcgcact ggagtgcaaa cccgtccggt tcgccctcca agtagttgat ttcaagggag
      541 aatggctcgt gtgtcacagc gccttcgaga atggcggtgc cgtgtttttc cgccgtcagg
      601 cgcttccccg gacaaggagg ttcggcgatg acgtatttgg tgaggcgacg attcgccatg
      661 gttctggcga tcgctctcct ttggccggaa gcacgtggcg aagacttgaa cgacgcctgg
      721 agccaggcga tcgggctgaa tgccggcctc caggctcggg agtttgatag cttcgccgcc
      781 gggatgaact tgggggcggc gcgatccgcg cggttgccgt cggtgagtct gacgtcgctc
      841 aacgcgtttt tgagtcagtc tcccgtgatc agggttccgg catccctcgc gggatcgggg
      901 gccttggcgt caaaaggtgg ctttccggcg atcggccaga atcagaccga catcccattc
      961 gtattcgcca acatcaacca gccgatctat caggggggcc gaattcgcgg caacatcgag
     1021 gcgtcgaagg cgggtgtcaa ttatcaaaag accgaggaat tccgcaccgt cctcgacctc
     1081 aagctgaccg tggccgaggc gtacgtgggt gtgttgcgag ccaggagaaa tctgggcgtg
     1141 ttgcggagca acgtcgccca gctttcgtcg tttctgcgcg atgtgaccaa ccgcaagaat
     1201 gaaggccggg cgactcgtaa cgaagaactc gcggccgagg tttccctggc caacgccaaa
     1261 cttcaagaga tcagcgctcg caaggacttg gatgcggcct gggccacgta caaccgctat
     1321 ctttaccggc cgccgagcag ggtggtcgac ctccaggaga tcatcggacc gccgtcgtcg
     1381 gcctcgttgg attcattaac cgcgcacgcg atcaatgaac aggattcttc gttcacgctc
     1441 aacgagccga tggccgcgga tttaaccggt cgcgccttgg aaacgcgccc cgaactccaa
     1501 gggctggccg aacgggccag gcaatccagc gccttggcca acgccacgat ggcgacaatc
     1561 aagccgcaag tgtcttttaa cgcgtcgtat atcgacctcg ggttgcagaa tttcaccaat
     1621 cccaatattt tcgcgggaac ctttcagcta tcgtggacgt tgttcgacgg cggtcaaacc
     1681 cgacgacgag cggaagccca gcgtttgcaa ggccgttcgt atctgaagca acgagcggac
     1741 gcctccgccg acgtcgtgct tcaagtccgc acccggatgc tcgacgtgga cgaatcacgt
     1801 cagcgggtga tcgtttcggg agccgcggtg gcttcggcca atgagaacat caaggtcgtc
     1861 accgagcggt atcgccaagg actgagtatc tacaccgagg ttctcgacgc cgaaaatcgc
     1921 cggattcaaa gcctgaacgg ctattttgcg gcggtttacg ataacgcgct tgcgaggttc
     1981 cggctccatc gagccgcggg cgacctctaa gctgggatgg gcaaggtcat gtcattgcgt
     2041 cgcgactcct aactgaacgt cgctaagaag tgttcgagga agcccgtctg tccccagaga
     2101 gttcgccggt gccgtgcgct gatactgacg gatcgaaatc gagaaaaatt catcgcacgc
     2161 gttcaagggc ccgcggcatt ctcactcgat cccgtatttg agcagctttt gccgcaacgt
     2221 ggcgcgatgg atccccagga tctgcgcggc cgcggcgcgg ttgtggccgc attcttccaa
     2281 gagagcgcgg aggaccggag gttcggcgag ctggaggaat tgctcataga ccgttgcgtc
     2341 accggatccg tcctggtcac gaaaagcgcg ccgggcccag agggcgagca ggccctggat
     2401 ttcgatcgta ttcgacggcg caatcaggtc cggcctcgct gtggcggtcg ggaggtgctc
     2461 cggccggatc ggctggccgc gggccacgat ggccgcatgt tcgatcgcgt tacgcagttc
     2521 acgcacattg cccgtccagg ctcggttgcg gagctcgtcg agcacatcgc cggtgagcgg
     2581 catcgctgcc acatttgcca gcggcacttg cctcaagaag tggtcggcga gagacggaat
     2641 gtcctcgcgc cgctcacgca ggggaggaag gtggatctga aaaacgctga ggcggaaaaa
     2701 caggtcttcg cggaactcgc cagccgccat aagggcaccg agagggcggt tggtcgccgc
     2761 gatgacccgg atgtcggtga accgaggccg ggcatcgccg accggcgtga cttcccggtg
     2821 ctcgattgtc ctcagcagct tgacttgaag gctgggcgac acgtcgccga tctcgtccaa
     2881 g
//
LOCUS       DATAQL010000608         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_62517, whole genome shotgun sequence.
ACCESSION   DATAQL010000608 DATAQL010000000
VERSION     DATAQL010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62517"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1191
                     /locus_tag="VHS97_10625"
     CDS             193..1191
                     /locus_tag="VHS97_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448699.1"
                     /translation="MTGRGPQVDGKKSMIRTLIIATTAAAALSASAALAQERSQGAIS
                     IPDFSGIWGNPYLYGIEPPLLGPGPVGNKVRRRQILDADGRPFPAAAAPLVSDARRLV
                     GDYANPILKPAAAEVVKKDGDLALSGAGYPSPRNQCWPQGVPFIFTNSALQLLQQPDR
                     ITMLYDEDHEVRRVRMNQPHPAQVTPSWYGNSAGHYEGDALVIDTVGIKIGPFSAVDQ
                     YGTPFSQALHVVERYRLIDREAAREAQQRGGQENPRNGAGAIAEGWAADPDYKGKGLQ
                     LQFTVEDAGVFTTAWSATKTYRRVFREWPESVCAENPHRYNTEKDPAVPTADKPDF"
     gene            complement(1381..1624)
                     /locus_tag="VHS97_10630"
                     /pseudo
     CDS             complement(1381..1624)
                     /locus_tag="VHS97_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759231.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1803..>2089)
                     /locus_tag="VHS97_10635"
     CDS             complement(1803..>2089)
                     /locus_tag="VHS97_10635"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX3448700.1"
                     /translation="FCFSAADAFGQTAKELVGSWTLISVTVNRAGEKVEPFGPNPKGT
                     MTFDSGGRFSIIVTRSDLPKFASNNREMGSSEENKAVVQAEASPILAPTT"
BASE COUNT          445 a          637 c          634 g          373 t
ORIGIN      
        1 atccccgccc tacgaccgga cacccgcctt ttgctccgcc ggcgccacgt caggatcgac
       61 cgaggaggtt gcaggccgac gaccgcctgg gccaaacccg gaacggcaca tgcgctgtcg
      121 tttccggctc ctttttgatg caggtgaact ggtgtaacgt tcgatccgag ttgccaggga
      181 agtcggcgcg tcttgacggg gcgcggcccg caagttgacg gaaaaaaatc catgatacga
      241 acactcatca tcgccacgac ggcagccgca gcgctgtccg cttcggcggc gctcgctcag
      301 gaacgaagcc aaggcgcaat atcgatcccg gatttctccg gcatctgggg caacccatac
      361 ctctatggca tagagccgcc gctgttgggc cccggcccgg tggggaacaa ggtgcggcgg
      421 agacagatat tggacgccga cggccggccc tttccggctg ccgccgcgcc gctcgtcagc
      481 gacgccaggc ggctggttgg cgattatgcc aacccgatcc tgaagcccgc cgccgcggaa
      541 gtcgtgaaga aggacggcga cctcgcgttg tccggcgcgg gctatccgag cccacgcaac
      601 cagtgctggc cgcaaggggt gcccttcatt ttcacgaatt cggcgctgca gttgttgcag
      661 cagccggaca ggatcacgat gctttacgat gaggatcatg aagtacggcg tgtgcgcatg
      721 aaccagccgc atcccgcgca ggtgacgcca tcctggtacg gaaattcggc cggccattac
      781 gagggcgacg cgctggtgat cgacactgtg ggaatcaaga tcgggccgtt ttccgccgtc
      841 gatcagtacg gcacgccgtt cagccaagcc ctgcacgtgg tggaacgtta tcgactcatc
      901 gatcgcgaag ccgcgaggga agcgcagcag cgcggcggac aggagaatcc ccgcaacggc
      961 gccggcgcca ttgcggaagg gtgggcggcc gatcccgact acaagggcaa gggactacaa
     1021 ctccaattca cggtcgagga cgcaggcgtc ttcacgacgg cctggtccgc gaccaaaacc
     1081 tatcggcggg tcttccgcga gtggccggaa agtgtctgtg ccgaaaaccc gcatcgatac
     1141 aataccgaaa aagaccccgc ggtcccgacc gcggacaagc cggacttctg agagttgcaa
     1201 gcgactgtga ccgccacgct cgctttgggt caaaggactt gcaacctgcg tttcagcacg
     1261 aaccgagatc gagaaatggt ggccgatcat caaggcagcg aacatcaagg gcgaataatc
     1321 cggttgcggt gggcgcattc tacaatcccg ctcagttgat tctgccggcc gcgacttcgg
     1381 caaccggcgc tcagaacgga tgccgtagca gtagccgaca atcagcatcc ggatcatgag
     1441 ttcggggtcg attgagggcc ggccgatctc gctgtagaag ggccccagct tctcgctaag
     1501 gttcagccat tatccgcgtc accaccggat tgatgcggcg aagaagatgg tccgtcggga
     1561 tacgaccttc gaggttgaag agatagaaca gctggctctg atcgaccttc tgccgaccca
     1621 tcatgacccg cttccgctgc cgagaacctc aggcagacgg aaccacgatt cgccgatcac
     1681 agcgagagac tttttcaaca gcatcggtca caaccggaaa ggctcggcac gtgcacaaat
     1741 tccaacccag ttcgggtacc tgctgccccc aacgtgaacg gtgaacatat gatcggcctc
     1801 gtctacgttg taggtgccaa aataggcgat gcttccgcct ggactaccgc tttgttctct
     1861 tccgagcttc ccatctcgcg gttgttggaa gcaaatttgg gaaggtcgga gcgtgtgacg
     1921 atgatagaaa agcgaccccc gctatcgaac gtcatggtgc ccttcggatt tgggccaaac
     1981 ggttcgacct tctcgccagc gcggttgacg gtgacggaga ttagcgtcca tgaaccaaca
     2041 agctctttgg ccgtttggcc gaacgcatcg gcggctgaga aacaaaaag
//
LOCUS       DATAQL010000609         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_62678, whole genome shotgun sequence.
ACCESSION   DATAQL010000609 DATAQL010000000
VERSION     DATAQL010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62678"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VHS97_10640"
     CDS             <1..1143
                     /locus_tag="VHS97_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448701.1"
                     /translation="ALLNRARALEGEALSIESKVKRLGVDAPAEWLAMAQDARRRQVP
                     ESDSGALGHRAFRAKLAAATSEAELKTLIEQIEAFFPGAAADKASGRVNLARWETPYT
                     DDPAGTYRDRETPPHVRKGFDRRLWTDATVRLLEIEAAQDLPAAIAASERASSLVPER
                     PNLAASILEKATRLARQDVGSLRLSEVKAVAAAIRERLRQPEEARTVLADWLKIQRDR
                     LSATDAEGPVDLANLYEELLDDHVTSVELLRKAWRIDPSSKAIAEALRSRGFRKVKDE
                     WVESAPASPVNPPAGANTPPVAMNSQGLLGLTADEVTARLAVKPNRVNFVGSKGQLIE
                     QRIYHLDTKRVRFVNLLHSPGELKPRVIADYTLPSSNMKGGVGHAR"
     gene            1655..2137
                     /locus_tag="VHS97_10645"
     CDS             1655..2137
                     /locus_tag="VHS97_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX3448702.1"
                     /translation="MARKDALLRLTSRLIARRDALRKALNGDLDSFRRFSELNAVGDN
                     VDAAVDSANDEICSQLVEIESRELGQIEHALQRIAAGVYGRCEFCSNKIAEARLNALP
                     YTNTCIDCQRENERVGQSRVLEPDSKRWAKVYEKSEEGETDSKINLSDFEMDFSETGR
                     "
     gene            2470..>3300
                     /locus_tag="VHS97_10650"
     CDS             2470..>3300
                     /locus_tag="VHS97_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3448703.1"
                     /translation="MLSRSSAVIISLTSLSAGIVLGILLGRGGPVVSAQVAGSGIDGP
                     VTAISGSQTKTLSRQKLADDATYDVLAKQYEQFQHINRTFELVAKAVSPSVVHIVAQK
                     TLRDEKTRKSRPIEETGSGVIIRSDRVPGLFVLTNHHVVYGSEPGKIRVLLSDSRSIS
                     VGKVWTDSKADIAVLKLDRDDLPAARLGNSDDAPIGTWVMALGSPFGLRHSVSQGIIS
                     ARGRQMEELSDIMVNQDFIQTDAAINPGNSGGPLVNMKGEVIAINNSIASNGGGNEGV
                     G"
BASE COUNT          710 a          963 c          945 g          682 t
ORIGIN      
        1 gctctcttga accgcgctag ggcactcgag ggtgaagccc tgagtatcga gtcgaaagtc
       61 aagcgtctgg gagtggacgc cccggccgaa tggctggcca tggcgcagga cgcgcgccgg
      121 cgtcaggttc ccgagtccga ctccggcgca ctgggccacc gcgcgtttcg ggccaagctc
      181 gccgccgcta cgagcgaggc cgagctcaag accctcatcg agcagatcga ggcctttttt
      241 ccgggtgcgg ccgccgataa agcgtcggga cgggtcaacc tggcgcggtg ggaaacgccg
      301 tacaccgacg atccggcggg cacctatcgc gatcgcgaaa ccccgcccca cgtcagaaag
      361 ggcttcgatc ggcggctctg gaccgacgcc accgttcgct tgctcgagat cgaggccgca
      421 caggatctgc cggcagcgat cgccgcctcg gaacgtgctt ccagcttggt ccccgagagg
      481 cccaacctcg cggccagcat actcgaaaaa gcaacacgct tggcccgcca ggacgtaggg
      541 tctctacgat tgagcgaagt caaggctgtc gccgcggcca ttcgcgaacg gctacgccaa
      601 cccgaagaag cgagaaccgt gctggccgac tggctcaaga tccagcgcga tcgcttgagc
      661 gcgaccgacg ccgaaggccc cgtcgacctg gccaatctct acgaagagct gctggacgat
      721 catgtcacgt cggtcgagtt gttacgtaaa gcatggagaa tcgacccgag ctcgaaggca
      781 attgccgaag cccttcgctc ccgggggttt cgtaaggtca aggacgaatg ggtcgagtca
      841 gcgccagcct cgcccgtaaa tccgcccgcc ggagctaaca ctccgccggt ggcgatgaat
      901 tcgcagggac ttctcggcct caccgccgac gaagtaaccg cacgcttggc ggtcaaaccc
      961 aaccgcgtca acttcgtcgg ctcgaaagga cagttgatcg aacagagaat ctaccatctt
     1021 gacacgaagc gggtccgttt tgtaaatctg ctccactccc caggcgaatt gaaacccagg
     1081 gtcatcgcgg attacacgtt acccagttca aacatgaagg gaggcgtcgg acacgcgcga
     1141 taatccgtgc aagtcacttc ttggcagtgc tttgaggtga ttgcttgatg accgttcacg
     1201 atcctgcctt ccgccatcgg cctgaccacg cctccgacaa ttctccgagt ttgacgcggc
     1261 gcgatgccag accttgcgac ctctgaacgg attcgtcgtc ccaaatttcg ggctggccga
     1321 tcctctacga agattttttc gattttttgg caaccccttg cccgctcgtg acgtactatt
     1381 ccaggtggat cgcggtcgac cgtgtccatc ccttgtcaat ggcgcataat gtgctagacc
     1441 ttgtcggtgg gagcgattcg ttttcgttcc attatcgtgt cgcgtcgatt cggcatactc
     1501 acaaggactc agcctcaccc agttcgagcc atcatcaatt tgaattgaaa gatttttcga
     1561 ttccatttgt tgactgggaa atgctggcac tgcggtctgt cggtccgatc ccgacaaaag
     1621 ctgatccgca ctgacgtaca ccaggaggtg tcatatggcc cgcaaagatg ccctgttaag
     1681 actaacatcc cggctaatcg cgcggcgcga tgccctgcga aaagcgctta acggcgacct
     1741 cgacagcttc cgaaggttct cggagctgaa tgcggtcggc gataatgtcg atgctgccgt
     1801 tgattcggcg aacgatgaga tttgctctca gcttgtcgag attgagagtc gcgaactcgg
     1861 acagatcgaa cacgccctgc agcgtatagc tgctggagtg tacggacggt gcgaattctg
     1921 tagcaataag atcgctgaag cacggttaaa cgcgctgcca tacacgaata cttgcatcga
     1981 ctgtcaacgc gagaacgagc gcgttgggca gtccagggta cttgagcccg actccaaacg
     2041 ttgggccaaa gtctacgaaa agtcggagga aggcgagacc gactcgaaga tcaatctcag
     2101 cgacttcgag atggatttca gcgaaactgg ccgctgacgg acagccctat cgcccgagca
     2161 gtgagcaatt agtagccgcc ggaaattctc cggcggcttt tttttgtcca aacgcccaca
     2221 tggcaatagc ttgccgaagg cgcgttttgc tcgttttttg acattgggtc aaggaggggg
     2281 gagtgtatcc aaaaatgcac gcaatgtttc aagccaagca acttcggcgc ctgcgcgcag
     2341 tcgatctttg acttggctgc ctgataggca gtatattagt aagacgcccg ccgtccgtaa
     2401 gagaaggatc ttcaaattac cgagcggatc tgcgtccctt caatccgccg ttggcgtgga
     2461 gtctggttga tgctttcccg atcttccgca gtcatcatct cactgacttc gctgagtgcg
     2521 ggaatcgtgc ttggaattct cctgggacgg ggcggccctg ttgtctcggc tcaagttgcc
     2581 ggctcgggaa ttgacgggcc ggtaaccgcg atatctggct cacagacgaa aaccttgtct
     2641 cgtcaaaagc ttgcagacga cgccacttat gacgtcctgg ccaaacagta cgagcaattt
     2701 cagcatatca atcgcacgtt cgagcttgtc gccaaggccg tttcgccctc cgttgtgcac
     2761 atcgtggctc aaaagacgtt gcgggacgag aaaactcgga agtcccgccc gatcgaggaa
     2821 actggctcgg gtgtcatcat acgtagcgat cgcgtccccg gcctcttcgt gttgaccaat
     2881 caccacgtcg tctatggctc ggaaccgggc aaaattcgcg tgctcttgag cgattcgcgc
     2941 tcgatttcgg ttggcaaagt ctggaccgac agtaaagccg acatcgcggt tttaaaactc
     3001 gaccgcgacg acctgccggc agctcggctg ggcaacagtg acgacgcccc gattggaacg
     3061 tgggttatgg ctcttggcag cccgtttggc ttgagacatt cggttagcca gggaatcatc
     3121 agcgctcgcg ggcgccagat ggaagagctt tccgacatca tggtcaatca agacttcatt
     3181 cagaccgacg ccgcgatcaa ccccggcaat tcggggggac ctctcgtcaa tatgaagggt
     3241 gaagtgatcg ccatcaacaa ctcgatcgca tcaaatggcg gtggcaatga aggtgtcgga
//
LOCUS       DATAQL010000610         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2504401, whole genome shotgun sequence.
ACCESSION   DATAQL010000610 DATAQL010000000
VERSION     DATAQL010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504401"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1791
                     /locus_tag="VHS97_10655"
     CDS             43..1791
                     /locus_tag="VHS97_10655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3448704.1"
                     /translation="MRMDPEHRLARAGLTTAGFSVWGLLTAAALVVGLTLLVDTVSTS
                     SATYDEVAYLKVAARWWRTGDQSEITRMGSPLLFWKLQQVPVLWALDHLGQGAWVEDP
                     IGNQRQLLPLVRLGSLWIWLLAATLTTLWSRHSLGPRAMALAAWLFALSPNLIAHGAL
                     ATMELPLVAATTAMCALFWRFLDTTKKTWFWSSAAAGGVAFSCKYTAILFPPILAVVW
                     WVALWRRGDERVLRPTRRVASYMLGFMLIMLLSNLVVTDFARIPLSKTEGRHPTLEKW
                     FGMKATATLTRLYETPLPQDWVGFATQVHHQASGGASYLWGERRTKGWWYYYFVALAF
                     KVPLGLWLLVCARLAFARSDARNQFTNSHIELLPLVVVLFLAITAVGSSRNYGMRYLL
                     PLAPLSIVWVSALAECKKPIWPGLAATAGLAGFALAIAASHPHELTYFNILAGGPRGG
                     RRILSDSNLDWGQGLCAIVRLQKEQPDFRDITFYYFGDTHPAYYGVAGRSYMINAALD
                     HTGLPGLDQVDTRYLAVSSSLQWGPWGPPGFFRDLDRLEPVCFSDDTTVAIYRTAELR
                     AVMAKRSNNDRRVLIR"
     gene            complement(1827..2393)
                     /locus_tag="VHS97_10660"
     CDS             complement(1827..2393)
                     /locus_tag="VHS97_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger Ran-binding domain-containing
                     protein"
                     /protein_id="HEX3448705.1"
                     /translation="MISVMIVCARCTLANHASDRYCASCGLPMGSLQPDAEAGSDALE
                     PYEHPEPADPDVTRLIAEFVRRTRHDANPSGRGWQLVVPLRLDRKQAVYVGPAGTDSE
                     GRAIMGLVSVCGPVNDRDCRTLLKLNARIVEGHFAVRVLRGEEYFVVIENLPVDATQS
                     VDAPRLVRHVAELADGLEDRLSRGRDIY"
     gene            complement(2452..3027)
                     /locus_tag="VHS97_10665"
     CDS             complement(2452..3027)
                     /locus_tag="VHS97_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448706.1"
                     /translation="MNTQKSLRRFASLGLAALAAALMLSGADDPKQTVNAGGLTFEAP
                     PAWKSSPTPPAGMRRAQLKVQPVDGDDYPAEMVVFAFPGGAGTVEANIERWRKQFKDK
                     NGNSPEPVTKKVKGKNVEVTRVETAGHYYPAQFPGRPAEPDRPDARLLGAIVIGDGTS
                     FFIRMVGPNKTMNKISSEFDELLGSLKLEAK"
     gene            3194..3991
                     /locus_tag="VHS97_10670"
     CDS             3194..3991
                     /locus_tag="VHS97_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX3448707.1"
                     /translation="MRRSRIIGLSVIWSCFLAHPALAQKKVPDGAKVFVLSGGQRQHH
                     GYRDQALYMAGALENTGRFQVTIGEDAAIVESPAIRKYDVLIVMADRRDDEFKFTTSQ
                     QEAIFDFVRSGHGYVSIHGADNGAKDWLPQWKEMLGGVFSHVGLPDGKTKKGQYTVKI
                     ADTNSPITRGLKDFPLKDELYYHIQMQPDVQALATVEYQGVDWPVAWTRTYGKGRVFH
                     TPLGHRDFGPDKDDPLRNPNLGKLIIQGVEWVAPGRPSAETAAKPAS"
     gene            complement(4101..4376)
                     /locus_tag="VHS97_10675"
     CDS             complement(4101..4376)
                     /locus_tag="VHS97_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448708.1"
                     /translation="MRNPGIQFKVWAMMIATAVIACFLAFEIFLFDVATRQLVMHADT
                     SSWKGIFMLWIAMNLPIIVVPLLLAYAYFHNRKCDEVDGHVDDDQDG"
     gene            complement(4421..>4870)
                     /locus_tag="VHS97_10680"
     CDS             complement(4421..>4870)
                     /locus_tag="VHS97_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 38"
                     /protein_id="HEX3448709.1"
                     /translation="VGSRMLDTLLVAGSESTRSFTLGVVLDLEHPFHAAQDLIAPPLV
                     VPIDDGPPSLGSSGWLARIDRKGVAVSHVGFASATGDDRGWGLVFHLLETAGQAGRCR
                     LRLFRNPLGARQADFLGETIVDLSIDGDAVLVDLTPHELARIEVTLG"
BASE COUNT          905 a         1568 c         1439 g          958 t
ORIGIN      
        1 cgggccggca gcgctcgccc ggcgacgtga ggactgacag tcatgaggat ggatcctgaa
       61 catcgtttag ctcgtgccgg cctgaccact gccggcttct cggtttgggg tttgctcacc
      121 gccgccgctc tcgtggtcgg cctgacactg ctcgtcgata ccgtctcaac gagttctgcg
      181 acctacgacg aagtcgccta tctgaaggtg gccgcacgct ggtggcgcac cggcgatcaa
      241 tccgaaatca cgcgcatggg atcaccgctt ttgttttgga agctccagca agttccggtg
      301 ctctgggcgc tcgaccacct gggccagggt gcatgggtcg aggacccgat cggcaaccag
      361 cggcagctct tgcccttggt gcgcttgggc tcattgtgga tctggctctt ggccgcgacg
      421 ctgaccacgc tatggagtcg ccacagcctc ggaccccggg cgatggccct ggctgcctgg
      481 ctctttgcgc tttcacccaa tctgattgcc catggcgcac tggctaccat ggagctgcca
      541 ttggttgcgg ccacgacagc catgtgcgct ctcttctggc ggtttctcga cacgaccaag
      601 aagacctggt tttggtcgag cgcggcagca ggcggcgtgg ccttttcgtg caagtacacg
      661 gcgatcctct ttccgccgat cctggctgtc gtctggtggg tcgcgctctg gcgccgaggc
      721 gacgagcgcg tgctccggcc cacgcggcga gtggcctcgt acatgctcgg tttcatgctg
      781 atcatgctgc tctccaacct cgtcgttact gactttgctc gcatcccgtt gagcaaaacc
      841 gagggccggc accccactct cgagaagtgg ttcggcatga aagcgactgc cacgctcact
      901 cgactatatg aaacgccgct cccgcaagac tgggtcggtt tcgcgaccca ggtgcatcac
      961 caggcttccg gcggcgccag ctatctctgg ggtgagcgcc gcaccaaggg ctggtggtac
     1021 tattatttcg tcgcgctggc attcaaggtg ccgctgggtt tatggctcct cgtctgtgcg
     1081 cgactggcgt tcgcccgctc ggacgcaagg aaccagttca cgaattcgca tatcgaatta
     1141 ctcccgctgg tggttgtttt atttctggcg atcactgcgg tcggctcgtc tcgtaactac
     1201 gggatgcgtt acttgctacc cttggccccg ttgtccattg tctgggtgtc ggccctggcc
     1261 gagtgcaaga aaccgatctg gccaggcctg gctgctaccg cgggcctcgc cggtttcgcc
     1321 ttggccattg ccgcatccca tccccatgag ctcacgtact tcaatatcct ggccggagga
     1381 ccgcgcggcg gccgccggat tctctcggac tcgaacctcg attggggcca agggctttgc
     1441 gcgattgtcc ggcttcaaaa agaacagccc gattttcggg acatcacctt ttactatttc
     1501 ggcgataccc atccggccta ctacggcgtg gccggccgct cttacatgat caatgccgcc
     1561 cttgaccata ctggactacc cggtttggat caggtcgaca cgcgctatct cgccgtttcg
     1621 tcgtcgctgc agtggggccc atggggcccg ccgggctttt tccgcgatct ggaccgcttg
     1681 gagcctgtct gcttctccga tgacacgacc gttgccatct accgaacagc cgagctgcgc
     1741 gccgtgatgg caaagcggtc gaacaacgat cgtcgggtcc tcatacgtta acgcggtgac
     1801 cttgagcggc gcgctgtcaa cagttcctaa tagatgtctc gtccccgcga aagccgatcc
     1861 tcgagcccgt ccgccagctc ggccacgtgt cggaccagcc tcggggcatc gacggattgg
     1921 gtcgcgtcga cgggtaagtt ttcaattacg acaaagtact cttcaccacg cagaacccga
     1981 accgcgaagt gcccttctac gatgcgcgca ttgagcttga gcagggtacg gcaatcgcgg
     2041 tcgttgactg gcccgcagac cgagacgagc cccatgattg cacgcccctc ggaatcagtg
     2101 ccggcgggtc cgacgtagac agcttgcttc cggtcgagcc gcaagggcac gacgagctgc
     2161 caaccccggc ccgaggggtt ggcatcgtgt cgagtccgcc tcacgaattc ggcaatcaga
     2221 cgggtcacgt ccggatccgc cggttcggga tgctcgtacg gctcgagcgc gtcgctgccc
     2281 gcctcggcgt caggttgaag ggagcccatc ggcaaaccac aactcgcgca atagcggtcc
     2341 gaagcgtggt tggccagcgt gcagcgtgcg cacacgatca tcacggaaat catggctcac
     2401 tcgctccttg gtcttttggc ggcgtgtgcc cgagaccacg ccccctgtcg ttcacttggc
     2461 ttcgagcttg agcgagccca aaagctcgtc gaactcggag ctgatcttgt tcattgtctt
     2521 gttcggtcca accatccgga tgaaaaagct ggtcccgtcc ccgatcacga tcgcccccag
     2581 cagtcgggcg tcgggccgat cgggctcggc cggccggcca gggaactgtg cagggtagta
     2641 atggcccgcg gtctcgacgc gcgtgacttc gacgttctta cctttgacct tcttggtaac
     2701 tggctccggg gagttgccgt tcttgtcctt gaactgcttg cgccagcgct cgatattggc
     2761 ttctacggtg ccggccccgc cggggaatgc aaagaccacc atctcggccg ggtagtcgtc
     2821 tccatcaacc ggctggacct tcaactgagc ccgtcgcatc cccgcgggcg gggtcgggct
     2881 cgacttccat gccggcggcg cctcgaacgt caatccaccg gcgttgacgg tctgcttggg
     2941 atcgtccgcc ccggacaaca tgagcgcggc tgccagtgcc gccaaaccca gggacgcgaa
     3001 acgccgcaga ctcttttgtg tgttcattgt tattgactca catgggaaac acgatcgtcg
     3061 acacgatact tgcgagccga ctcgggtcgg gctacgctac gacaaataca gtggacaaac
     3121 cgctagagac attgtgatcg aacgcgatgg actgatcccc cccacgaccg actgaaagac
     3181 aggagtaccg cccgtgcgtc gatctcgtat catcggtttg agcgtgatct ggtcttgctt
     3241 cctggcgcat ccggccctgg cgcaaaagaa agtgcccgat ggcgccaagg tcttcgtgct
     3301 ttccggcggg cagcgccagc atcacggata ccgcgaccaa gcattgtaca tggccggcgc
     3361 gctcgagaat accggccggt tccaggtaac aatcggtgaa gacgccgcca ttgtggaatc
     3421 ccccgccatc cgcaaatacg acgtcttgat cgtcatggcc gaccgccgcg atgacgaatt
     3481 caagttcacg accagccagc aagaggcgat tttcgatttc gttcggtccg gccacggcta
     3541 tgtctcgatc catggcgcgg ataacggggc caaagactgg cttccccagt ggaaagagat
     3601 gctcggcggc gtcttctcgc acgtcggact gcccgacggc aagacaaaaa agggtcaata
     3661 caccgtcaag atcgccgata ccaacagccc catcacccgg ggactgaaag actttccttt
     3721 gaaggacgag ctctattacc acatccagat gcagccggat gtgcaggcgc tggccacggt
     3781 cgagtatcaa ggggtcgact ggccggtggc ctggacccgg acttacggca agggccgggt
     3841 tttccacacg cccctgggcc accgcgactt cgggcccgac aaagacgacc cgcttcgcaa
     3901 ccccaatctg ggtaagttga tcatccaagg ggtcgaatgg gtcgcgcccg gccgcccctc
     3961 cgccgagacg gcggcgaaac cggcatcgtg acccgatcga tgaatcgccg tgcgccaggg
     4021 ccagcggatc ccgcgcgacc aagagcgtgt cggtctcatg gacgggtcca cactcgaacg
     4081 agacaggccc aacattgtct tcagccgtct tgatcatcgt caacgtgccc atcgacttcg
     4141 tcacattttc gattgtgaaa gtaagcataa gccaacaaga gcggtacgac gataatgggc
     4201 agattcatgg cgatccacag catgaaaata cccttccagc tcgacgtatc cgcatgcatc
     4261 acgagctgtc gagttgcaac gtcaaacaag aatatctcaa atgcaaggaa gcatgcgatg
     4321 accgccgtgg cgatcatcat cgcccagact ttgaattgaa tccctgggtt acgcactgac
     4381 cctcctccgc ccgagaagcc agctcaccgt cgccccgaaa ttagcccaat gtcacctcga
     4441 ttcgcgccag ctcgtgcggg gtcaaatcga cgagcaccgc gtcgccgtcg atcgacaggt
     4501 ccacgatcgt ctcgcccagg aaatcggcct gacgcgctcc gagcggattg cggaacaatc
     4561 gcaagcggca gcgccctgcc tggccggccg tttcgagcaa gtgaaaaacg aggccccagc
     4621 cgcggtcgtc gccggtcgca ctcgcgaacc ccacgtgcga gacggcaacc cccttgcggt
     4681 caatccgcgc cagccacccg ctggagccga gcgatggggg gccatcgtcg atcgggacga
     4741 ccagaggggg tgcgatcaaa tcctgcgccg cgtgaaaggg atgctcgagg tcgagcacca
     4801 cccccagcgt gaacgaccgg gtggattcgg aaccggcaac caagagtgtg tcgagcatgc
     4861 ggctgccgac
//
LOCUS       DATAQL010000611         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2200305, whole genome shotgun sequence.
ACCESSION   DATAQL010000611 DATAQL010000000
VERSION     DATAQL010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200305"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VHS97_10685"
     CDS             complement(<1..779)
                     /locus_tag="VHS97_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448710.1"
                     /translation="MPPITVNRRRFLGCSAAASLALSQGNLAEAAGIDAEARPVRLGV
                     IGVGNRGTALLRALLELPGTPIVAVCDPEPKHRQRGQGIIEKARGQRPDAYDDPRRVL
                     DRTDIDAVIVAIPCDAHEAVYRDAIMASKHLYAEKPLALTLAGCDRLIAAADQAPNLA
                     VHVGFQRRSNPRFQDGVELIGRGELGALIEARATWTSSNGPMNGHGGWLGRRDRSGDW
                     MVEHAVHIWDILQWFMRESPVRASGWGRRGLFAKTDPLRDVT"
     gene            965..2593
                     /locus_tag="VHS97_10690"
     CDS             965..2593
                     /locus_tag="VHS97_10690"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX3448711.1"
                     /translation="MAKHIFVTGGVVSSLGKGLTCASIGMLLEQRGLRVRLQKFDPYI
                     NVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFTHVPLTRDCNYTTGKIYLSVIQKE
                     REGRFYEGKTVQVIPHVTDEIKEAIRRVAGDDVDVVITEIGGTVGDIEGLPFLEAIRQ
                     FALDIGRENCLYIHLTLVPYLKAAGELKTKPTQHSVGVLRQIGIQPDVLICRTEHPIA
                     TDDKDKIALFCNVDRKAVIEERDKQFSIYEVPLSLVNHGLDEILVKRLHLRAGPLDLT
                     AWRDMVERIMHPKHEVRIAVVGKYMKHRDAYKSVYEALDHAGIANRTRVLVVRVEAEE
                     VDARGADALLGGVDGILVPGGFGMRGIEGKIEAIRFARNRKVPFFGICLGMQCAVVEF
                     ARDVLGLENANSTEFEQTTDHPVIWLMDEQQSVRERGGTMRLGSWPCQLARGSLAHEA
                     YGQDLIHERHRHRYELNNAYRKSFQEHGLIATGTSPDGTIVEIMERRDHPWFVSVQFH
                     PEFKSKPTKAHPLFRDFIAAALRNRENSRAGAER"
     gene            2597..3733
                     /locus_tag="VHS97_10695"
     CDS             2597..3733
                     /locus_tag="VHS97_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3448712.1"
                     /translation="MRVVARDFRDARWIELDLREGYIDLVRPVEGPEETEAEDDWVAP
                     AFWDIQLNGRWGHSFSDPELTIDQVASIVRAQGPLGTSRLCPTLITAPADHLIHGVGT
                     IAAACERYPEVAARVLGIHLEGPFISELEGYRGAHPAGSIIDPDWGLFQALQDASGGR
                     IVLVTLAPERAGAIEFIRRATLAGVMIAIGHTAADGATIRAAIEAGALLSTHLGNGIA
                     SQLPRHPNPIWLQAGSKTLLASFIADGHHLDLATLRVLALAKGPEQTILISDASPLAG
                     LPPGAYGEWAVDPSGKIVVAGTPYLAGSNQGLDVGLQNLLFATNWTFKQAIASVTTNP
                     ARLLGRPMPRMAAGEPADLVVFRRPEPTHFALRRVCVGGRWELG"
BASE COUNT          790 a         1290 c         1247 g          780 t
ORIGIN      
        1 gtcacatcac gcaacgggtc tgtcttggcg aaaagcccgc gccggcccca accgctggcc
       61 cgaaccggcg actcgcgcat gaaccattgc aggatgtccc agatatgcac ggcgtgttcg
      121 accatccagt cgcccgaacg atcgcggcgg cccagccatc cgccgtggcc gttcatgggg
      181 ccattgctgc tggtccaggt ggcacgagcc tcgatcaacg cccctagctc gcctcggccg
      241 atcagctcga ccccgtcttg aaacctgggg ttcgaccgcc tttgaaatcc aacatggact
      301 gccaggttgg gcgcttggtc tgccgcggcg atcaggcgat cgcagccagc cagggttaac
      361 gccaatggct tctcggcgta gaggtgctta gaggccataa tcgcgtcgcg atagaccgct
      421 tcgtgggcgt cgcaaggaat cgcaacaatg accgcgtcga tgtccgttcg atcgaggacg
      481 cgcctggggt cgtcgtaagc atcaggccgc tgcccgcgcg ccttctcgat aatcccctgc
      541 cctcgttgcc gatgcttggg ctcggggtcg cagacggcca cgattggcgt tccaggcaat
      601 tcgagcaagg ctcgcaaaag cgcagtgccc cgattgccga caccgatcac tcctaaccgt
      661 accggtcttg cttcggcatc gatgcccgcg gcctccgcca ggtttccctg ggacaaggcc
      721 aggctcgccg ccgccgagca gcccagaaac cggcggcgat tcactgtgat cggaggcatc
      781 ggtcagctcc acggcagatt gaagtagcac aaacttccag tttattctat cccgtttcca
      841 ttccaggttc gaggggatga gtcgtcattc cgcccgggcg aggagtcgtc atcgcggcct
      901 ctcatggtat actagacact tggaatgtgc catacaaatg aacagacaat aggcaaaatc
      961 cgcgatggcc aagcacattt tcgtgacggg aggcgtggtc agctctctgg gcaagggatt
     1021 gacctgcgct tcgatcggca tgttgctcga gcaacgggga ctacgcgtcc ggctccagaa
     1081 attcgatccc tacatcaacg tcgatcccgg gacgatgagt ccgtaccagc atggtgaagt
     1141 gtacgtgctc gacgatgggg cggaaaccga cctggacctg ggtcactacg aacggttcac
     1201 gcacgttccg ctcacccgcg actgtaacta cacgacgggc aagatttacc tttccgtcat
     1261 ccaaaaggaa cgcgagggac ggttttacga gggaaagacc gtccaggtga tcccgcacgt
     1321 gaccgatgag atcaaggagg ccattcgccg cgtggctggg gatgatgtgg acgtggtgat
     1381 caccgagatc ggcggcacgg tcggcgacat cgaggggttg cctttcttgg aggcgatccg
     1441 ccagttcgcg ctcgatatcg gccgcgagaa ttgcctttat atccacctga ccctggtacc
     1501 ctacttgaag gccgccggcg agctcaagac caagcccacc cagcactccg tcggcgtgct
     1561 ccggcagatc ggcatccagc cggatgtctt gatctgtcga accgagcacc cgattgcgac
     1621 cgacgacaaa gacaagatcg cgctcttttg caacgtcgac cggaaggcgg tcatcgaaga
     1681 gcgcgacaaa cagttcagca tctacgaggt gccgctctcg ctggtcaatc atgggcttga
     1741 cgagatcttg gtgaagcgtt tgcacttaag agccggcccg ctggacttga ccgcctggcg
     1801 cgacatggtg gaacggatca tgcatcccaa gcatgaggtg cggatcgcgg tcgtcggcaa
     1861 atacatgaaa catcgcgacg cgtataaatc ggtctatgag gcactcgatc acgctggaat
     1921 cgccaatcgc actcgtgttc tcgttgtgcg cgtcgaggcc gaggaggtcg acgcccgcgg
     1981 cgccgacgca ttgttgggcg gcgtggatgg catcctcgtc ccgggcggtt tcgggatgcg
     2041 cggcatcgag ggaaagatcg aagccattcg cttcgcacgg aatcgcaagg tcccgttttt
     2101 tggaatctgc ctgggcatgc aatgcgccgt cgtggaattc gcccgcgacg tgctcggtct
     2161 cgaaaacgcc aacagcaccg agtttgaaca gaccaccgac cacccggtga tctggctcat
     2221 ggacgaacag cagtcggtgc gcgagcgagg aggcacgatg aggctcggct catggccttg
     2281 ccagcttgct cgtggcagct tggcccacga ggcctacggt caagatctga ttcatgaacg
     2341 acaccggcat cgctacgagc tcaacaacgc gtatcgcaaa tcgttccagg agcacggact
     2401 catcgcgacc ggcactagcc ccgacggcac gattgtcgag atcatggaac ggcgcgatca
     2461 cccgtggttc gtatccgtcc agttccaccc cgagttcaaa tcgaagccca ccaaggccca
     2521 tccgctcttt cgcgacttca tcgccgcggc tcttcgcaat cgcgaaaaca gcagagcagg
     2581 cgcggagcgt taggtgatga gagtcgtcgc ccgcgatttc cgcgacgcgc gctggatcga
     2641 gctcgacctg cgcgagggct atatcgacct ggtccgaccg gtcgagggcc ccgaagagac
     2701 cgaagccgag gatgactggg tcgcacccgc gttctgggac attcagctga acggccggtg
     2761 gggacactca ttctcggatc ccgagctgac gatcgatcaa gtcgcctcga tagttcgggc
     2821 acaggggccg ctgggaacat cccggctctg tcctaccctg atcaccgcac cggccgatca
     2881 cctgatccac ggagttggaa cgatcgccgc ggcgtgcgag cggtatcccg aggtggccgc
     2941 ccgggttctc ggcattcatc tcgaggggcc atttatctcc gagctggagg gttaccgcgg
     3001 cgcacaccct gccgggtcga tcattgatcc ggattggggc ttgtttcaag ctcttcaaga
     3061 tgcatcgggc ggccgtatcg tcttggtaac cctggctcca gagcgagccg gggccatcga
     3121 gtttatccgc cgcgccacat tggccggcgt gatgatcgcc atcggtcaca cggccgccga
     3181 tggtgccacg atacgagccg ccatagaagc gggcgccctg ctcagcactc atttgggcaa
     3241 cggcattgct tcacagcttc cccgccatcc caacccgatc tggctgcagg cggggtcgaa
     3301 aacgctactt gcatccttca tcgccgacgg ccatcacctg gatctggcga cacttcgcgt
     3361 tctggcattg gcgaaagggc cagagcaaac cattctgatc agtgacgcca gcccgctcgc
     3421 cgggttgcca ccgggcgctt acggggagtg ggccgtcgat ccatcgggca aaatcgtcgt
     3481 ggcgggaact ccctatttgg cgggctcgaa ccaggggctc gatgttggac tgcagaatct
     3541 gctgttcgcc accaactgga cattcaagca agcgatcgcc tcggtcacga ccaatcccgc
     3601 acgccttctc ggccgaccga tgccgcgcat ggcggcaggc gaacccgccg acctggtcgt
     3661 cttccggcgg ccggagccga cgcattttgc cctccggcgc gtttgcgtag gcggccggtg
     3721 ggagctcggc tgacgcagtt cgatttcagc ctggatgaat cccagtcggt cgtacatgtc
     3781 agggtgcgtg cgggcggtag aagggtcgtt ccatttccat gccaacggcg gcgaaacgcc
     3841 gaaaatgaag tggaccctgg gcccgatgaa tcgaattcga ggcgcccttg acagaccgcc
     3901 gttcccctga tcagattgtc cgttggtcat cttgaccatc catccggtcc acgttccgtc
     3961 gggcctggtg aggttcatgg cttcctcgcg aacgcggcat caataggtca tcgccccagg
     4021 atgcgacaat tcgctcgaca aatcctgatg gccacgctcg tcgccctcta tgggggcgtg
     4081 accgccttgg gtccggcgct ccacgcg
//
LOCUS       DATAQL010000612         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1131555, whole genome shotgun sequence.
ACCESSION   DATAQL010000612 DATAQL010000000
VERSION     DATAQL010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131555"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VHS97_10700"
     CDS             <1..173
                     /locus_tag="VHS97_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3448713.1"
                     /translation="WLNLIEGFFSKLARSVLRHIRVESKEELKDRLMAAMDYFNQEPV
                     VHSWSYGLDQAK"
     gene            complement(1037..1378)
                     /locus_tag="VHS97_10705"
     CDS             complement(1037..1378)
                     /locus_tag="VHS97_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448714.1"
                     /translation="MPPSRAGWRALHCVGDLVLAELPPPDGRFAGVVVVESYTHIHAS
                     MKVAVSGADRGLDFAGVHELESGRQIPVTMINRLLDDGDVRRLQEAIMLRKPPGPSVQ
                     RPTPARRVGKR"
     gene            complement(1444..>1716)
                     /locus_tag="VHS97_10710"
     CDS             complement(1444..>1716)
                     /locus_tag="VHS97_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448715.1"
                     /translation="VSAQHHQGDEPSDVRVPASRFRSSTRLAQALRWPSITETSINVR
                     LPPGELAALDAFVKQDDPKPSRPEALRRFAQNALSTRPAKRGVTGL"
BASE COUNT          314 a          518 c          532 g          352 t
ORIGIN      
        1 cgtggctcaa cctcatcgag ggcttctttt ccaagctcgc tcgctccgtg ctgcgtcaca
       61 tccgcgtcga atccaaagag gaactcaagg atcgtctcat ggcggccatg gattacttca
      121 accaggaacc ggtcgttcac agttggtcct atgggctcga ccaggccaag tgatatgatt
      181 cgagccatga aatcgatgta ctaggatatc gtggggcgcg gtctttgctg gcgtggttgt
      241 ggcactcgcc acccaactcc tgctcaacct cctcggaatg ggcgctggcg ctgccacatt
      301 gacccccggt gcgggggata gtccatcagc gacatcgttc tctattggcg ctggaatatg
      361 gttcgcagtg tccagcattt tggccgcact tgccggaggc tacgccgctg ggaaactggc
      421 tggcgttccg aatgagtcaa ccgggagctg gcatggtttg acgacctggg cacttgcaac
      481 gctggtggtt ttttatctcc tgacttcaag cgctcggaca aattctcgac aacgcggcga
      541 aatactcgcc gccggcatca ctaatcaacg tgcgaggccg tgttgaatcg ggacaattcg
      601 tgatttccgt cgctgaccaa ggtgccggtc ttgacgccga cgaccgcact ctgcttggac
      661 agaagttctt ccgtgggaat cgccactccc aggtgacttc gggcctagga ttgggattct
      721 ggatcgccaa cgccttcgtg gcagcaaaca acggcaccat cgaggtgacc agcgagggcg
      781 ccgagaaggg catgacggtt acaattcggt tacccttgtc gacgaacgat cggtcggccc
      841 atgtccccga agaaggggca aatgcgaata gtctcccagc ttgggcttga ggccccgccg
      901 aaggccgcgt aaaggcctac ccgtggcgtc accgatgacc aggtcgtagt tcctggggcc
      961 atcttgttgg ccgagagcgc gacgtggtgt ggccattcgc ggcgcaggcg ggcttgggag
     1021 atttcaccct tgcgggtcat cgtttgccaa ccctacgtgc gggcgttgga cgctgcaccg
     1081 acggccctgg cggtttcctg agcatgatgg cctcttgcag ccggcggacg tcgccgtcgt
     1141 cgaggagccg gttgatcatg gttaccggaa tctgccgccc gctctcaagc tcgtggacgc
     1201 ccgcgaagtc aagccctcga tcggcccccg acaccgccac tttcatgcta gcgtgaatgt
     1261 gcgtgtagga ctcaaccact actacgccag cgaaccggcc gtcgggtggc ggtaattcag
     1321 ccagtacaag gtcaccgacg caatgtaatg cccgccaccc cgcccgggag ggcggcacgc
     1381 tgtcacccta tctgggcggc cgatctctcc ggacaggttg gtcggccgga gccgcgttcg
     1441 ggctcataac ccggtgactc cgcgcttcgc cggtcgcgtt gagagtgcgt tttgcgcgaa
     1501 ccggcgcaac gcttccggcc gcgacggctt gggatcatcc tgcttgacaa aggcatcgag
     1561 cgccgcaagc tcgcccggcg gtaggcgcac gttgatggat gtctcggtga tagaaggcca
     1621 acgtaaggcc tgtgcgagcc tcgtggatga gcgaaaccgc gaagccggca ctctgacatc
     1681 agatggctca tcgccctgat ggtgttgtgc tgagac
//
LOCUS       DATAQL010000613         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4642644, whole genome shotgun sequence.
ACCESSION   DATAQL010000613 DATAQL010000000
VERSION     DATAQL010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4642644"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VHS97_10715"
     CDS             complement(<1..739)
                     /locus_tag="VHS97_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448716.1"
                     /translation="MRHYQSVVIIWSLLISGMALGGDGASAQSAPAEGAAKPKPTAEV
                     PAAPQEPSKTEEAKLLSAVREALERNAQEIKSLKEQYAKDMAEQQKKVEAQQKQIESL
                     ERSAQVLQDRLKTQVAAPNVAGAQNAQGQDRQKQLTDLQQKEIGLLEQQSKLVAEQLE
                     QQAPAIEKLQGQTATLESREKQAAQRDSELDNAYNSLNDSFDAQQRNPPWLPAPVKEL
                     FTPSGNNATPISIYSTMSTRYNLFNQKR"
     gene            complement(1247..1894)
                     /locus_tag="VHS97_10720"
     CDS             complement(1247..1894)
                     /locus_tag="VHS97_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3448717.1"
                     /translation="MDSSDRPKLSDRTRKSWCAIVSCVALLAVTAATRTADAGCQCGK
                     TWPHLLCRQHDQYHTPGPGDISDVDTGGVWYWMRSPEQERRVVMNLFNRYCIRCHGVD
                     GRGVWDIPDVPDFTNTTWQATRPDSYRTRVILEGRGAVMPSFRGTLTLEESAAMARYL
                     HTFVPGTEVSRPDLGEAATGNKPAASSPTAGKRAALPPLPPSPATPPTTPSPFNR"
     gene            complement(2130..3068)
                     /locus_tag="VHS97_10725"
     CDS             complement(2130..3068)
                     /locus_tag="VHS97_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3448718.1"
                     /translation="MSRATLPGPTSAVPAATAPPVSPPTPTFRVGRANLRHAYDLALV
                     GGLGALFGLYLYVELVKAESVYVRDALAGLLIGGSLGVFLSAYSAFRDGAWLKLARAV
                     AWGAPAAALGGAIGLVAGEVVIGLLRGGLVGRALSWAVLGLGIGLGQGLADRSRQRLI
                     YGLIGGGLGGLVGGFLFEWLRVALADRLDISQALGVVVLGAGLGLCLALVEQALRRAW
                     VQVQSGRQEGRIYLLARPRCRLGLDERAEIGIFGDPAVARQHAEIEATTTGYILRHLA
                     PQAFTRVNGTPVRDLQSLKDGDRIEIGKTLLVFRQR"
     gene            complement(3065..>3608)
                     /locus_tag="VHS97_10730"
     CDS             complement(3065..>3608)
                     /locus_tag="VHS97_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX3448719.1"
                     /translation="HELIRVFLLLDVSSSMTGQPLIEAQTAAREFLSKCDLTTMEVGL
                     ISFSTLVALQSVATSNARRLHAAVHRLEAEGSTNLTDALEMARGQLVSGDRKRYVVIL
                     TDGYPDAPESALEQSLAAREQGIEIVAIGTGDADRDYLRRLASSDQASIFARSGELVQ
                     AFGHIARVIAEGGRGLRVLS"
BASE COUNT          707 a         1087 c         1096 g          718 t
ORIGIN      
        1 cgcgcttctg gttgaacagg ttgtaacggg tagacatggt gctgtagatg gaaatcggcg
       61 ttgcattgtt tccgctcggc gtgaacaact ctttcacggg ggcaggcaac caaggaggat
      121 ttcgctgctg tgcgtcgaac gagtcgttca gactgttata ggcgttatca agttcggagt
      181 cgcgctgggc ggcttgcttt tctcgtgatt ccagcgtggc agtctgtcct tgcaattttt
      241 cgatcgcggg tgcctgctgc tcgagctgtt cggccaccag tttcgactgc tgttcgagca
      301 aaccaatctc cttttgctgg agatccgtaa gctgcttctg ccggtcttgc ccctgggcat
      361 tttgggcccc ggcgacattg ggagcggcga cttgagtctt gagccggtct tgtaatacct
      421 gagcagaacg ttccagagac tcgatctgtt tctgctgcgc ctcaaccttc ttctgttgct
      481 ccgccatatc cttcgcgtat tgttctttga gagatttgat ctcctgagcg ttacgctcga
      541 gtgcctcgcg gacggctgag agcagctttg cctcttcagt cttggagggc tcttgaggag
      601 ccgccggaac ttcggcggtc ggcttcggct tcgccgcacc ttcagctggc gcagactggg
      661 ctgatgctcc gtcgccaccc aacgccatgc cgctaatgag cagagaccag ataattacca
      721 ctgactggta atgccgcatg cggatttccc tcaggaacaa tgccgtccga acgcgcgtat
      781 gatccgaatt gaattgcgga gagtacggac gagatgatgc gaggtgtatg aatcctgcac
      841 cggtgaccga gatggccggc agtgatgtta tcgaaccggt agggatggca aattgaggcg
      901 aaattgacgg ctgtagggtt ttttccgcct ttatcgattt tggcgcgctg aatgctcact
      961 gatccgaatt aagtggaaag accctcaatc aactgattta tccagtcact aagaatctca
     1021 acgccgaatt ttttggtatg cctgcacatc gtgcgtgcgt gcatcagccg cacaatgatg
     1081 gcgatggcgt ctagaatccc tcggccgttc gaatgcggtt tagcgggaag acgcgaaaaa
     1141 gtcgaagggg atggccctgg attggtgcca tccccattct tcctgtctcg gaggaaaaca
     1201 aggccggttc tgactgcgtt caggatcgcg aacgctccaa gaatcgtcac cgattgaagg
     1261 ggctcggcgt ggtaggcggg gttgccggac tgggtggtag tggtggtagc gcggctcgtt
     1321 taccagcggt cggactgctc gccgcgggct tattccccgt ggcggcttca ccgaggtcgg
     1381 gtctcgatac ctccgtacct ggaacgaagg tgtggaggta gcgggccatc gcagccgatt
     1441 cttccagcgt cagagtgccc cggaacgaag gcatgaccgc cccgcgtccc tccagaatga
     1501 ctcgcgtacg gtatgaatcg gggcgcgtcg cttgccaggt ggtgttcgtg aaatcgggga
     1561 cgtcgggaat gtcccagacg ccccggccat ccactccgtg gcaccgaatg caataccggt
     1621 tgaagaggtt catgacgacc cgccgctcct gctcggggct tcgcatccag taccataccc
     1681 caccggtgtc gacgtccgag atgtcaccag gccccggagt atggtattga tcgtgctggc
     1741 ggcataaaag atgtggccag gttttcccgc attgacagcc ggcgtccgca gtgcgagtcg
     1801 cggccgtcac cgccaagagc gccacgcaag acacgatcgc gcaccacgat tttcttgtac
     1861 gatcggaaag cttcggacga tcgctcgagt ccatgactcc tcctaatcag tctcactggc
     1921 atcgcttcct gagcgatcgg ggtattccta gaagcaccgg tgtgaccggt cgttccggct
     1981 cgcatccata tgttctgtcg gaagaacgcc ctcgttactt taagaacttc tgtccacgag
     2041 gctgagatgc ttgttccggc atgtcaccga accggggaaa agcggtgaag acgcgaatgc
     2101 cgaggaacat cggcgatagt tgcatccaat caacgctgcc gaaaaactag gagggttttc
     2161 ccaatttcta tgcgatcgcc gtccttcaac gactggaggt ccctgaccgg cgttccattg
     2221 actcgcgtga acgcttgcgg tgcaaggtga cgcaagatgt aaccggttgt cgtcgcctcg
     2281 atctcagcgt gttgacgagc gaccgccggg tcaccaaaga tgccgatctc ggcgcgttca
     2341 tccagaccga ggcggcagcg tggacgggcc agcagataga tgcggccttc ctggcgtccg
     2401 ctttgcacct ggacccatgc gcggcgcaac gcttgctcga cgagagccag gcaaagtccc
     2461 aatcccgcgc ccaagacgac gactcccagc gcctggctta tgtcgaggcg atcggccagg
     2521 gccactcgca accattcaaa aaggaagccg ccgaccaacc cccccaggcc gccgccgatc
     2581 aggccgtaga tcaagcgctg gcgagaccgg tcagccagac cctgcccgag cccgatcccc
     2641 aggcccagca ctgcccacga cagagccctc cccaccagac cgccgcgcaa gagaccgatc
     2701 acgacctcgc ccgcgaccaa gccaatcgcg cctccgagcg ccgccgccgg agctccccat
     2761 gcgacggccc gtgccagctt gagccaggca ccgtcgcgga aagcactata cgcgctcaag
     2821 aaaacgccga gagatccccc gatcaagagt ccggccagtg cgtccctaac gtaaactgat
     2881 tcggctttga ccagctcgac atacagatag agcccgaaga gcgcacccag gccgccgacc
     2941 aaggcgaggt cataagcgtg acgaagattg gcacggccga cgcggaacgt cggcgtgggt
     3001 ggtgaaaccg gcggcgctgt cgcggccggc acggccgatg tcgggcctgg taaggtggct
     3061 cggctcatga taagacgcgc aggccgcgcc ctccctcggc gatgacgcgg gcgatgtgtc
     3121 cgaacgcctg aaccaactca ccgctccgcg caaagatcga cgcctgatcg ctgctggcga
     3181 gccggcgcag gtagtcgcgg tcagcgtcac cggtgccaat ggccacgatc tcgatccctt
     3241 gctcgcgggc ggccagtgac tgttcgagcg cgctctcggg ggcatcggga tagccatcgg
     3301 tcaagatcac gacataccgc ttacgatcgc cggagacaag ctggccgcgc gccatctcga
     3361 gggcgtcagt caggttggtg cttccctcgg cctcgagacg gtgcacggcc gcatggagcc
     3421 gccgcgcgtt gctcgtcgcc acggattgaa gcgcgaccag ggtggagaac gaaatcaggc
     3481 cgacttccat ggtcgtaaga tcgcattttg acaaaaactc gcgggcggcg gtctgggcct
     3541 cgatcagggg ctggcccgtc atgctggacg agacgtcgag cagcaagaag acgcgtatca
     3601 gctcgtgc
//
LOCUS       DATAQL010000614         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_522018, whole genome shotgun sequence.
ACCESSION   DATAQL010000614 DATAQL010000000
VERSION     DATAQL010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522018"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..565)
                     /locus_tag="VHS97_10735"
     CDS             complement(104..565)
                     /locus_tag="VHS97_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448720.1"
                     /translation="MLASSQFFVCLWVLFEGAPPLGSAPEAVTLRGKVVTLAAALESR
                     GLGLKADAEPIAKQVVLLAEDGSITPLLSDEASRALFLDERLRNCRAEIQGRRFQGMP
                     YLQVSTFKVERDGRLQAPESFCDVCAISVHHPQSCPCCQGPRVLRMKPDRR"
     gene            851..2023
                     /locus_tag="VHS97_10740"
     CDS             851..2023
                     /locus_tag="VHS97_10740"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3448721.1"
                     /translation="MSATHATQSTAPQAPVLYMALELSLATWKLAFTIGSGQKPRIRS
                     VPAGALSCLTDEIELAKKRFGLPEGATVISCYEAGRDGFWIHRFLIKGGIQNIVVDSA
                     SIEVNRRKRRAKSDRLDATKLVSMLIRWHNGEKKVWAAVRIPTVADEDRRQLHRELID
                     LKAQRTEHANRIKGLLAAFGLRVVVDTKVPERLAALRQWDNQEVPPALRERVLREFER
                     WQLVERQIHDLESKRLEAIRTDQAPEVEKVRQLLRLSGIGENGAWLLVREFFGWRQIR
                     NRRELASLAGLAPTPYDSGGSRCEQGISKAGNRRVRWIMVELAWIWLRHQPTSDLSKW
                     FLRRFGQGNGRVRKVGIVALARKLLVALWKYLETGEVPAGVVLDGKKKVLRLKKAS"
     gene            2575..4179
                     /locus_tag="VHS97_10745"
     CDS             2575..4179
                     /locus_tag="VHS97_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3448722.1"
                     /translation="MAQTHKSKHSSSKRAQIIHKPRGVISPRVQAAGPQHFGIISVDC
                     AKARSKWMLTDFYGSILVPPTEVEHTQSCLSSMIEGLHAALELAGIRDQIVVIERTGR
                     YHRPIQHAFTKGGFEVRIVHPLTTKQHRQPANPGNKTDDTDLAAIYRGAVNGFGLLEP
                     EPDPISVRLQLLARHRRDVVEKTVALRSQIHEHLHAFMPGYSHCFADLFDAHIPLWVA
                     RNLHSAAAIVAAGVPGIIGQLRQAGIRVHLPTVEKVVAWARSAPAGEDESSLHWNILI
                     DLDDDRLLKARRMRAIEVELAALLVRTPYLLLLGIPGINVVSAAEFAGEMGPIERYPS
                     SRAITGRAGLFPSRYQSDQVDYADGKLVRCANRSLRRAIMMIADNLIKCNEHFRVLAA
                     GWRLQGADPRAMRVRVAGRFSRIAYQMIAGRKAYRHPCCQQRDYVLKKLITFHSEHGT
                     GPDQLKRDLDGALSQLPRSEYREEAIPLADELELVLNKRGKGPRPLAEILPVVLAKMG
                     VKPVRSSESGDAILTEPSGRMSPTTS"
BASE COUNT         1042 a         1417 c         1439 g          877 t
ORIGIN      
        1 cgcgctaatt caagagatag cttgttcaga aaaccaacag ccattcccgc acgcgagaga
       61 ccggacgccc ggcctgttcc gcccgatgcc ccggactatc ggatcagcgg cgatcgggct
      121 tcatgcgtag caccctcggc ccttggcagc aagggcaact ctgtgggtga tggacactga
      181 tagcacaaac atcgcaaaaa gattcagggg cctggagccg gccgtccctc tcgaccttga
      241 aggtgctgac ctggagataa ggcattccct ggaatcgcct gccctgaatc tcggcccgac
      301 aattccgtag ccgttcgtcg aggaagagcg cacggctcgc ctcgtcggag aggagcggcg
      361 tgattgatcc atcctcagcg agaagcacga cctgctttgc aatcggctca gcatcggcct
      421 tgaggcccag accgcgcgat tcgagggcgg cggccaaggt cacgaccttt ccccgcaggg
      481 tcaccgcctc gggcgcggag ccaagtggtg gagcgccctc gaatagaacc caaaggcaaa
      541 cgaagaattg tgacgaagcc agcatcggcc actcttctcc acgtccttgg gaagcaacga
      601 gttgagtgcg cgtttcacac cgcctacact gaaattataa cggcgctggc gcttacaacc
      661 aagcgatttt cgccggtgcg ccgaaaagag gcgccggagc caccgggggc gcgtggtaga
      721 agcagccgcg ctggctaatc gcaacagaag aaaaaggggt cagataccat gccatagagc
      781 ggtgtcaacg tgagtcgctg aggataagct ccagttagta tttccaaacg ctagttcaag
      841 gagcttaccc atgtcagcga cccacgcgac tcagtctacc gcgccgcaag cacccgtgct
      901 ctacatggct ctcgagctga gcctggcgac ctggaagctc gcgttcacga tcggctcggg
      961 gcagaagcca cgaatccgtt ctgttccggc gggcgcgctc tcctgtctca cggacgagat
     1021 cgaactggcc aagaaacgat tcggccttcc cgaaggtgcc accgtgatct cttgctacga
     1081 ggccggccgc gatggtttct ggattcatcg attcctgatc aaagggggaa tccagaacat
     1141 cgtcgtcgac tccgcgtcga tcgaggtaaa ccgacgcaag cgtcgcgcca agtccgatcg
     1201 cctcgatgcc accaagctgg tctcgatgct catccgctgg cacaacggcg agaaaaaggt
     1261 ctgggccgca gttcgtatcc ccaccgtggc cgacgaggat cggcggcaac tccaccgcga
     1321 gctgatcgac ctgaaggccc agcggaccga gcacgccaac cggatcaagg ggctgttggc
     1381 ggcgttcggc ttgagggttg tggttgacac caaggttccg gagcgattgg cggcactccg
     1441 gcagtgggac aatcaagagg tcccgccggc acttcgtgag cgagtcttgc gtgaattcga
     1501 gcgctggcaa ctcgtcgaac ggcagattca tgacttggag agcaagaggc tcgaggcaat
     1561 ccgaaccgat caagccccag aagtggaaaa agtccgtcaa ctgctccgac tcagtggcat
     1621 cggcgagaac ggcgcgtggc tgctggtgcg tgagttcttc ggatggcgtc agatacgcaa
     1681 ccgccgtgaa cttgcgagcc tggccggtct ggcgccgaca ccttacgaca gcggaggaag
     1741 ccgttgtgag caagggatca gcaaagccgg caatcgccgc gtgcgatgga tcatggttga
     1801 gctggcctgg atctggctgc gtcaccagcc cacaagtgat ctcagcaagt ggttcctgcg
     1861 gcgattcggc cagggaaacg gccgagtgag gaaagtgggc atcgtagccc tggctcggaa
     1921 actgctggtt gcgttgtgga agtatctgga gaccggtgaa gtgccggcgg gagtggtgct
     1981 ggatgggaag aagaaggtac tgcgattgaa gaaggcgtct tgacctaggg ggtttggcaa
     2041 ctgagggccc tgagaagaac gagttccagc cggtcccggg agtgaatcac gagcagacag
     2101 ggctggagga ggcgaccgaa attaggtcgg gctttccgca aggaagccgt gggtcagatg
     2161 ggctcgcctc caccagttct ggcttcgagt cgtcggtaga cggatcagta actggtgatt
     2221 gatcacttac gatcaacacg aatagaagac cgtgccggcc tcaaggtcgg cctcgtcgtg
     2281 aagccaccag ccactgtcgc ttgcgagtga attccacgag accaggagga agactttacc
     2341 aggtgcgatc cagaggcaac gaagtaggta aggtgcaaca gggacgatcg ggatgggaag
     2401 cagctaactg gcaacttggg ggcgggacca gaggccccag ttcccgcccc caaaccccca
     2461 cccccttccc tctgaaatgt ggcaagttag agctttttat tctaactctg aatgccatgc
     2521 agacttccag cggggaattg ggtctccccc aactttctcc tggaggtctt tgtcatggct
     2581 cagactcaca agtcgaagca cagctcctca aagcgtgcgc aaatcatcca caaaccccga
     2641 ggcgtgatca gcccgcgtgt ccaagcggct ggcccgcagc acttcggcat catctctgtc
     2701 gattgcgcca aggctcgctc caaatggatg cttactgatt tttacggctc tatcctcgtt
     2761 ccaccgaccg aggtcgagca cacgcaatcg tgcctcagtt ccatgatcga gggcttgcac
     2821 gccgctctgg aactggccgg aatccgtgac caaatcgtgg ttatcgagcg gaccggacgc
     2881 taccaccgcc cgattcagca tgccttcacg aaggggggat ttgaggtccg gatcgttcac
     2941 cctctgacca ccaaacagca tcgccagccg gcaaaccccg gcaacaagac cgatgacacc
     3001 gacctggccg ccatctaccg cggtgccgtc aacggcttcg ggctgctcga acctgagccc
     3061 gatccgatct cagtacgtct tcagttgctc gcccgacacc gccgggacgt cgttgagaaa
     3121 accgtcgcac tgcggtccca gattcacgag catcttcacg cctttatgcc gggatatagc
     3181 cactgcttcg cggatctctt tgacgctcat atccccctgt gggtcgcgcg gaatctgcac
     3241 tcagcggcgg ccattgtcgc cgccggcgtg ccaggcatca tcggccaact tcggcaggca
     3301 ggcatccgcg ttcacctgcc cacggtggaa aaggtcgtcg cctgggcccg ctcggcccca
     3361 gcgggtgaag acgagtcgag tcttcactgg aacatcttga tcgaccttga cgacgatcgg
     3421 ctcctgaagg ctcggcgcat gcgggcgatc gaagtggaac tggcggcctt gttggtgcgc
     3481 acgccgtacc tgctcttgtt gggcatcccc gggatcaacg tggtttcggc cgccgagttt
     3541 gcgggagaaa tggggccgat cgagcgctac ccaagcagcc gagcgatcac gggacgagca
     3601 ggattattcc catcgcgcta tcagagcgac caggtcgact acgcggacgg caaattggtt
     3661 cggtgtgcca accggagtct gcgccgggcc atcatgatga tcgcggacaa cctgattaag
     3721 tgcaacgaac acttccgcgt gctggcggca ggttggcggc tgcagggggc agatccccgc
     3781 gcgatgcgtg tccgagtcgc cggccgattc agccggattg cctatcagat gatagccggc
     3841 cgcaaggcct accggcatcc gtgctgtcag cagagagatt atgtactcaa gaagttgatc
     3901 acatttcaca gcgagcatgg gacaggtcca gatcaactca agcgcgacct cgacggcgcg
     3961 ctgtcccaac ttcctcggtc ggagtatcgc gaggaagcga ttccgctggc cgatgagctc
     4021 gagttggttt tgaacaaacg gggcaaagga ccgcggccgt tagcggaaat cctacccgtc
     4081 gtcttggcca agatgggggt gaaaccagta agatcatctg aatcagggga cgcgattctt
     4141 acggagccgt ccggccgaat gagcccaaca acgtcgtaag tcctctcagg cgcgaagccg
     4201 tagagaagcg tggcgctctg tcggaccacc aacgtcggat aaggctgcgt ggggtcgctc
     4261 ccctgatatt tcagaatggc gagacgtggt cttcgaatcg gcagagacgt ggctcatcaa
     4321 gcacgacgcc tacagatgca ttgttgtaca ccgccagtct ggcggagcag ggatcgtccg
     4381 cgcatggatc gtaaaaccgc gaccagacaa ttccccccat ggctgtcttc tctgttcacc
     4441 tcgatgtcga ccggcccggt tctcggaacg accgaatcac cgacgcgacc tgcacgctaa
     4501 atccggaagc gcaccaagac acgaatgaag tactgagcat acttagaatg cgagctcgac
     4561 gatccgcctg tgtgcttcca atgaacccgg ctcgtcgaga aacgatcacc gtccgctccg
     4621 aagagagcga aaaaggcaaa agagaaaaac ccctgaccgg ggcttgactt ccgcgggtca
     4681 tagaagacct aatccagggt gtcagaccta atttcaagtt cgggctggac ccttggtcac
     4741 agctcctcga cttcaaaccc gtccagggtg tcaga
//
LOCUS       DATAQL010000615         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_367787, whole genome shotgun sequence.
ACCESSION   DATAQL010000615 DATAQL010000000
VERSION     DATAQL010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367787"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VHS97_10750"
     CDS             complement(<1..588)
                     /locus_tag="VHS97_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3448723.1"
                     /translation="MQSLAASSAEAGSSQDRPLGTMVTYGYPDIEFDDELSLAIELGA
                     SLLEILPHWGRLLDAAMAREQAGDRGLLIHSAHGCWGGRTIRARRVDLGSVDASTHGE
                     SLDDLKACIDWLAAAGGKCLVVHPGGLSDPDEWTGRRMALSRGPLQLADHARGTDVVV
                     CVENMPPGVYPGSRMAELAALLRELDRSNLALALDT"
     gene            829..1230
                     /locus_tag="VHS97_10755"
     CDS             829..1230
                     /locus_tag="VHS97_10755"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX3448724.1"
                     /translation="MARKPVTFETVREIAARLPGAVEGISYGTPAFRVGKSLFVRQHQ
                     DGESLVVKMDRDERAMRIKADPETFYITDHYLNYPWILVRLSTVRRDDLRELLEDAWR
                     QSAPKALLSSDSGAAAETTRPPQRKRRGDKT"
     gene            complement(1242..>2360)
                     /locus_tag="VHS97_10760"
     CDS             complement(1242..>2360)
                     /locus_tag="VHS97_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3448725.1"
                     /translation="KAIPTALSGVQVSELLPNHARLLDRMSIIRSLHHDNGDHFAAAH
                     WMLTGYLGSNAINMAPQYPSAGSIVAKLKGAKRPGMPAYVGLPHTHSVGLAPGYHGGA
                     YLGVGYNPFNADGDPSSEKYHVPNLSLPAGVDAPRLTGRRGLLSAFDRVRRTALSSSS
                     IEGLDRFEQEAFSMVLGDRARQAFDLRKEDPRLRDRYSRHQWGQSTLLARRLVEAGVR
                     FVTLTFGGWDYHSSLERGMKRVLPILDAAVGTLIEDLEGRGLLDSTMVIVMGEFGRTP
                     RMNKTGVPGSDPVPGRDHWGKVMSVLVAGGGIARGQVVGSSNSRGEVPKDRPLRPQDL
                     IATVYHQLGINPSTTFLNRTGQPIKIGSDGEVIRELVG"
BASE COUNT          488 a          751 c          704 g          417 t
ORIGIN      
        1 agtgtcaagc gccagcgcca ggttggagcg gtcgagctcc cggagaagcg cggcaagttc
       61 ggccatgcgg ctgccggggt aaacgccagg cggcatgttt tcgacacaaa caacgacgtc
      121 cgtcccgcgg gcgtgatcgg ccagctgcag cgggccacgc gacagcgcca tgcgccgccc
      181 ggtccattcg tcgggatcag acaagccgcc aggatgcaca accaagcatt tgccgcccgc
      241 cgcggccagc caatcgatgc acgccttcaa gtcgtcgagg gactcgccgt gagtagacgc
      301 atctaccgat cctaagtcga cccggcgggc acggatcgtt cgcccgcccc agcagccatg
      361 agcgctgtgg atcaatagac cgcgatcgcc ggcttgctcg cgcgccatgg ccgcgtcgag
      421 cagccggccc caatgaggca agatctccaa caacgaggct ccaagctcga tggcaagact
      481 gagctcgtcg tcgaactcga tgtctggata gccataggtc accatggtgc caagcggtcg
      541 atcttgagac gatccggctt cagccgaaga tgcagcaagc gattgcatgt tttgtggact
      601 ccactacaac gtcgcagtcg caaatgtggt cacgatatca acgccggcat gatcagccga
      661 caaccgcgcc gaaactgaca cgccagtagg ccggcttctt atcgcagcgc gttgacgatc
      721 attgactctc attgtacttc ttgccatctc acaaaccgcg cgtcaccata acgaccatcc
      781 acggggcgag accagcgtgg gcgaacatta agaattcgga agggctcgat ggcacgaaag
      841 ccggtcactt ttgaaacggt ccgcgaaatc gccgccagac tgccgggcgc ggtcgaaggg
      901 atatcgtacg gcacaccggc gttccgggtc gggaagagcc tgtttgtccg ccaacaccag
      961 gacggcgaat cactcgtcgt caagatggat cgggacgagc gcgcaatgag gatcaaggcc
     1021 gacccagaga ccttttacat cacggatcac tatctcaact atccgtggat actcgtgcga
     1081 ctctcgacag tccgtcgcga cgacctgcga gaacttctcg aggatgcttg gcgccaaagc
     1141 gcgcccaagg cgttgctttc atcggactcc ggcgcggcgg ccgaaactac tcgccctcca
     1201 cagcgcaaac ggcggggcga caagacctga aggttgcgat tttagcccac cagctcgcga
     1261 attacctcgc catcggagcc gatcttgatc ggctggccgg tccgattgag gaacgttgtc
     1321 gagggattga tcccgagctg gtgatacacg gttgcaatca ggtcctgcgg ccgcagcggc
     1381 cggtctttgg ggacctcgcc gcgcgagttg gatgagccga cgacctggcc tcgcgcgatg
     1441 ccgcctcctg cgaccaagac gctcatcacc tttccccagt ggtcgcggcc cggcacgggg
     1501 tcggaacccg gaacgcctgt cttgttcatg cgcggcgttc ggccgaattc gcccatgacg
     1561 atcaccatcg tcgagtcgag caggccacga ccttcgaggt cttcgatcag cgtgccaact
     1621 gccgcgtcga ggatgggcaa tacgcgcttc attccccgtt ccaggctcga gtgataatcc
     1681 cagcctccga acgtcaaggt cacgaaccgt acgccggcct cgaccaatct acgggccaaa
     1741 agcgtgcttt ggccccactg atggcgtgag taccgatccc gcagtcgcgg atcttccttg
     1801 cgcaaatcaa acgcctgtcg ggctcggtct cccaggacca ttgaaaacgc ttcctgctcg
     1861 aatcggtcga gaccctctat cgaactggaa ctaagggcag tccgccgcac tctgtcgaac
     1921 gccgaaagta agccacggcg gcccgtcaac cgcggcgcat caacgccggc cggcagtgac
     1981 aggttgggca cgtggtactt ctccgaactg ggatcgccat cggcattgaa tgggttgtag
     2041 ccaactccca gataggctcc tccgtgatag cccggggcca atccgacgga gtgggtatgg
     2101 ggaagcccaa cataagccgg catccctggt ctcttcgctc ccttgagctt cgcgacgatc
     2161 gagcccgccg agggatactg gggggccata ttaatggcgt tggagccaag atacccggtc
     2221 aacatccagt gcgcggccgc gaagtgatcg ccgttgtcat gatgcagcga gcgtatgatc
     2281 gacatccggt ctaacaggcg ggcatggttg ggaagcaact cggaaacctg cactcccgag
     2341 agcgcggtcg ggatcgcctt
//
LOCUS       DATAQL010000616         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3270624, whole genome shotgun sequence.
ACCESSION   DATAQL010000616 DATAQL010000000
VERSION     DATAQL010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270624"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VHS97_10765"
     CDS             complement(<1..884)
                     /locus_tag="VHS97_10765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3448726.1"
                     /translation="MSRSAAPCRLIRTRTSRWVTLCLVAGAGSFIAWVAMAFSREDIE
                     AIESPLALVVARQLESSAGGVYGPYGRGNPRVLIHAPLYYRLATVAAWPLWRAGFDPV
                     TASLAAGRLLSALGFLMTMAAAYGLGRVGGLPSRAGYWAGLLVASTPVHSGLPLEVRP
                     DMLAVGLQSTGILLVLWALDAQRAALRKLAAAFACFGVALCIKQHFVMAPLISLFLLV
                     WNRARCRLGLVSILRYVLIAFTIVVLYYSMEEWVTGGRMSRSILVAAGNVGSVHPADW
                     PSAGNLLLALIWKCVGLIL"
     gene            complement(957..1562)
                     /locus_tag="VHS97_10770"
     CDS             complement(957..1562)
                     /locus_tag="VHS97_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX3448727.1"
                     /translation="MRWLHIYLSMFGLATVLFFSVTGITLNHPNWVFGQVERRREAEG
                     QVELKWISREITATNSQAGDQSDRSKEVAKLDVVEHLRKTQAIRGALTEFRVDESECM
                     VSFKGPGYSADAFIDRESGHYNLTEIDHGLVALVNDLHKGRDTGPIWSMLIDISAGLM
                     TLISLTGLVLLFYLKLRRVPGVIVALVGTVVIVLVFRFWVP"
     gene            complement(1636..3204)
                     /locus_tag="VHS97_10775"
     CDS             complement(1636..3204)
                     /locus_tag="VHS97_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEX3448728.1"
                     /translation="MSAIVASLGCSPLVAAEDFAFYHENVLGTSLALHVRCDSEAAAR
                     RAEDRVVGEIDRLAAIFSGYDQGSEFSRWQAAPKGPFPVSRPLYEVLLASETWRAKSG
                     GAFDTRAGAFSRLWTGCAQLGRLPSEAETRAAKELLTTPAWRLVPMTQLAERLSDCPV
                     SLDGIAKGYIVEQACEKALQDAAEVRGLVLNVGGDLRVRGEIDQTIGIAAPWADSESS
                     EPFVNIAVTDKSVSTSGNAQRGFMINGKWYSHVFDPRSGLPVDRVRSATVVADRGVDA
                     DALAKVCGVLEPNDSVRLINSLAGVDCLILTADGRVAKSAGWHTLERPEPISLPVAAR
                     QDRGPAEKAAADRGTADSKAASRPSWDPEFELVVNFEINHPEDQQGRYRRPYVAIWVE
                     DGEGMPVRTLALWVSMGGSGPFQWLPDLKRWFKSEQDRKAAEKKDIFFTVSRPTRPPG
                     KYKVIWDGKDNHGNQLAGGDYTVTVEAAREHGTYQSMRKQVALSNKPFTEDLKGNVEI
                     RSASIEYRRKAAAK"
     gene            3203..>3514
                     /locus_tag="VHS97_10780"
     CDS             3203..>3514
                     /locus_tag="VHS97_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448729.1"
                     /translation="MRQMPNRDDCGMIVGAAPRGGVLGYSIPWVLINDKDIGAAGQGW
                     WRVKTHVLAVFAGIADQMTTGLSWLTLCVVLHAFPVERVAVDGSIGLSQWRDHAGRAG
                     SR"
BASE COUNT          720 a         1083 c         1055 g          656 t
ORIGIN      
        1 aggatcaaac ccacgcattt ccagatcagc gccagcaaga gattgccggc ggagggccaa
       61 tccgcaggat gaaccgatcc gacattccct gctgcgacca ggatcgagcg agacatcctg
      121 cctccggtaa cccattcctc catgctgtag tagagcacga cgatcgtgaa tgcgatcaat
      181 acatagcgca aaatcgacac gaggccgagc cggcaccgag ctcggttcca cacaagcaaa
      241 aagagactga tcagaggggc catgacgaaa tgctgcttga tacagagggc cacgccgaag
      301 caggcaaatg cagccgccag tttgcgaaga gcggcccgtt gcgcgtcgag tgcccaaagc
      361 acgagcagga tgccggtcga ctggagtcca acagccagca tgtcgggccg aacctcgagc
      421 ggtaacccgc tatgaacggg tgtggacgcg accagaagac cagcccaata accagctctg
      481 gacggcaagc cgccgacgcg tcccaggccg taggcagccg ccatcgtcat caagaaaccg
      541 agtgcggaca gtagccggcc cgcggcgagt gatgcggtca cgggatcgaa ccccgcgcgc
      601 cagagcggcc aagccgcaac tgtcgcgagg cggtaataaa gaggtgcatg gatcaagact
      661 cgcggattcc ctcgtccata agggccatag acccctccag cgctcgattc caactggcgg
      721 gcaaccacga gtgccagagg cgactcaatc gcctcaatgt cttctcggga gaaagccatc
      781 gcgacccatg cgatgaaaga gcccgcgccc gcgacaaggc aaagtgtcac ccagcgcgac
      841 gttcgggttc gtatcagtcg gcacggggcg gcggaccggc tcatcggcga cacccccttt
      901 tccgtccgga agcccgccgc gttgttacct tccggatcac tgcgcgggac ccgatgtcac
      961 gggacccaga accgaaagac aagaacgatc acgaccgtgc cgaccagcgc cacaatcacg
     1021 cctggaacgc gcctgagctt gagataaaag agcaatacca gcccggtgag cgagatcaga
     1081 gtcatcaacc cagcagagat atcgatgagc atcgaccaga tcggccccgt gtcgcgccct
     1141 ttgtggaggt cgttgacgag tgcaaccaat ccgtggtcga tttcggtcag attgtaatgg
     1201 cccgactcgc ggtcgatgaa agcgtccgcc gaataacccg ggcctttgaa cgacaccatg
     1261 cattcgctct catcgacgcg aaattcggta agggcgccgc ggattgcctg tgtctttcgc
     1321 aggtgttcga ccacgtcgag cttcgcgact tccttcgatc ggtcgctttg gtcgccggct
     1381 tgcgaattcg tggcggtgat ctcccggctt atccacttga gctcgacctg accttcggcc
     1441 tcgcgccggc gctcgacctg gccgaagacc cagttggggt ggttgagagt aatccctgtt
     1501 acgctgaaga agaggacggt ggccagacca aacatcgaca ggtagatgtg cagccatcgc
     1561 atgagcttcg cgaaacgaat gctcagcttg cgtcgggacg agtgggtgtg agccgaggat
     1621 ggcaagatcg gagggctact tagccgcagc cttgcgacga tactcgatcg aggcggatcg
     1681 gatctcgaca ttgcccttca agtcctcggt gaatggcttg ttggaaagag cgacttgctt
     1741 gcgcatgctt tgataagttc cgtgttcgcg cgcggcctcg accgtaacag tgtaatcacc
     1801 gcctgcgagc tgattgccgt gattgtcttt gccgtcccag attaccttgt atttgccggg
     1861 cggcctggtt ggacgcgaca cggtaaagaa tatatctttc ttctccgcgg ctttgcgatc
     1921 ctgttcgctc ttgaaccatc tcttcaagtc aggcagccac tggaacggcc ccgaaccacc
     1981 catcgagacc cagagcgcta aagtgcgcac gggcatgccc tcaccgtctt cgacccatat
     2041 cgccacgtag ggccgccgat accgcccctg ctggtcttcg gggtggttga tctcaaaatt
     2101 gacgaccagc tcaaactcgg gatcccacga ggggcgggac gccgccttgg aatcggccgt
     2161 cccccgatcg gccgcagctt tttcagcagg accgcgatct tgccgtgcgg ccacgggcag
     2221 tgagatcggc tcgggccgtt cgagagtgtg ccacccggcg ctcttggcaa cccgcccatc
     2281 ggccgtgaga atcaggcagt cgactccggc cagcgaattg atcaatcgca cgctgtcgtt
     2341 gggctcgagc acgccgcaga ccttggcgag tgcatccgcg tcgacgcctc gatcggcaac
     2401 caccgtcgcg cttctaacgc gatcgaccgg cagtcccgat cttggatcga agacatgaga
     2461 ataccacttg ccgttgatca tgaacccgcg ctgcgcattg ccgctggtcg agacggactt
     2521 gtcggtcact gcgatattga cgaagggttc tgacgactct gagtctgccc agggggcagc
     2581 aatcccgatc gtctggtcga tctcgccccg cacccggaga tcgccaccga cgttcaagac
     2641 gagtcctcgc acttccgcgg catcttgtaa ggctttttcg caggcctgtt cgacgatgta
     2701 acccttggcg atcccgtcga gactgacggg gcaatccgac aggcgctcgg ctagctgcgt
     2761 catgggaacg agtcgccacg ccggcgtcgt caggagttcc ttcgcggccc tggtctccgc
     2821 ctctgacggc aggcggccga gttgggcaca gcccgtccag agtcgcgaaa atgctcctgc
     2881 ccgagtatcg aaggcgcctc cgcttttggc tctccacgtc tcgctcgcca acaacacctc
     2941 gtatagcggc cttgagaccg ggaacggacc cttcggggct gcttgccagc gggagaattc
     3001 gctcccctga tcgtagccgc tgaaaatggc ggcgagccgg tcgatctcgc cgaccacgcg
     3061 gtcctcggcc cggcgcgcgg ccgcctcgct atcgcaacga acgtgcagcg ctagggaagt
     3121 tcccaggacg ttttcgtgat agaaggcaaa gtcttccgcg gcaaccagag gcgaacagcc
     3181 taaactcgcg acgatcgcgc tcatgaggca gatgccgaac cgtgatgatt gtggcatgat
     3241 tgttggcgcg gctccaaggg gcggggtctt ggggtattcc attccctggg tgctgataaa
     3301 tgataaagat attggcgcgg cggggcaagg atggtggcga gtaaagactc acgtcttggc
     3361 cgtgttcgcc ggcattgccg accagatgac caccgggtta tcctggctaa cactttgtgt
     3421 cgtccttcat gcctttcccg tagaaagggt cgcggtcgat ggaagtattg gcttgtctca
     3481 atggcgagat catgccggtc gagcaggctc gcgt
//
LOCUS       DATAQL010000617         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3727575, whole genome shotgun sequence.
ACCESSION   DATAQL010000617 DATAQL010000000
VERSION     DATAQL010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3727575"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..3309
                     /locus_tag="VHS97_10785"
     CDS             166..3309
                     /locus_tag="VHS97_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX3448730.1"
                     /translation="MLNALIDFSLKNRFVVLLLAGLLIVIGVRSARRLPLDAFPDTTP
                     VQVQINTVAPELSPEEVERLITFPVEYALGGLKGIEEVRSVSKFGFSQVVAIFADGID
                     VYFARQQVNERLGEAKLPEGIRRPAMGPVATGLGEVYHYLLTSENPEYRLAELRTLQD
                     WVIRPRLRRVPGVAEINAWGGLEKQFEAQADPAKLAKYGLTLDDVIRALKQNNQNVGG
                     GYVVRSGEASLVQGIARTTSVDQIADIVIESHDGVPILIRDVADVEIGHAIRRGGVTA
                     RGNGEAVLGLAFMRMGENPRNVTNALDRAMDDVKKSLPPGVTIRVVYKRTDLVDAVLT
                     TVERNLFEGAILVIAVLFAFLGNLRAGLIVASAIPLSMLFAVTVMERIGIAGSLMSLG
                     AIDFGLVVDSSVVMVENCVRRLAHDRSNRTKLAIIRAAAIEVRKPTMFGELIIMIVYL
                     PILTLEGIEGKLFRPMALTVIFALAASLVLSLTLMPVLASLGLPRRISDKETLVDRLA
                     HWLFQPILRWGLRHPLSTLCLVAMLTVGTTILGLRLGSEFVPRLNEGSLVINTIRLAG
                     VSLEESLRYGTHIEGFLKKRFPDEIEDIWTRTGSAEVATDPMGMELSDIFITLRPRSG
                     WTRAKTQDELVSAMAQVTKVLPGMRAVYTQPIEMRINEMVAGIRADLGIKLYGEDLET
                     LKAKAEEIEKIVKSIPGAADTTTEQVTGLPVLRIHVDNEALSRFGVPAQQVLDAIKAV
                     GGIDVGEIIEPGRRFPLVVRLPMAYREDPQALEQISIPTASGQRLPLTQLVRIEDSTG
                     PSTIQRDWGERRIIVQTNVRGHDIGSFVKEAQERIGREIAFPVGYTVEWGGQFEHLQR
                     AEQRLYVVVPLALTLILSLLYLTFRSLRDAVMIFSGVLFARVGGILGLYIMGLPFTIS
                     AGVGFVALAGASILEGLILVSSIRDRLAHGVPKREAIEQARLARLRPVLMTGTVASLG
                     FVPMMLATGIGAEVQRPLATVVVFGMVCDTFLTMLALPVLYLLFGKGPLAETENDQEP
                     PDETTIEATEGLQAVRT"
BASE COUNT          638 a         1073 c         1049 g          691 t
ORIGIN      
        1 acgataccca gcggcaaaga ggacacattg gaagtgattt ggggactcaa gcccggtcag
       61 gaagttgtca cgactggagc ttttctgctg aagactgaaa taatgaaggg ctccatcggg
      121 gccggatgct gcgattgatc gcatcgccat gcgaggaacc gcccgatgct caacgccctc
      181 attgatttca gcctgaaaaa tcggttcgtc gtgctcttgc tcgccgggct ccttatcgtc
      241 atcggggtcc gctcggcaag gcgcctgccg ctcgacgcct tccccgacac gacaccggta
      301 caggtccaga tcaacaccgt cgctcccgag ctgtcgcccg aagaagtcga gcgtctcatc
      361 accttccccg tcgaatacgc cctgggtggg ctcaaaggga tcgaagaggt ccgctcggtc
      421 tcgaaattcg gcttctcgca ggtcgtcgcc attttcgcgg acgggatcga cgtctacttt
      481 gcccggcagc aggtcaacga gcgactcggc gaagccaaat tgcctgaagg catccgccga
      541 cccgcgatgg ggccagtggc gaccggattg ggcgaggtct atcactacct gctgaccagc
      601 gaaaatcccg aataccgcct ggccgagctg cggacgctgc aagattgggt gatccggccg
      661 cgtctccggc gagtgcctgg cgtcgcggaa atcaacgcct ggggtgggct cgagaagcag
      721 tttgaggccc aggccgaccc ggctaagctc gccaagtacg ggctcacgct cgacgacgtg
      781 atacgggcgc tcaaacagaa taatcaaaat gtgggaggcg gctacgtcgt ccgctcgggt
      841 gaagccagcc tggtgcaggg catcgcccgc accacgtctg tcgaccagat cgccgacatc
      901 gtgatcgaat cccatgacgg cgtgccgatc ctgataaggg acgttgccga cgttgaaatc
      961 gggcacgcca tccggcgggg cggtgtgacc gcgcgaggga acggcgaagc cgtcctgggg
     1021 ctcgctttca tgcggatggg cgaaaacccg cgcaatgtga ccaatgccct ggaccgagca
     1081 atggacgacg tgaagaaatc gcttccgccc ggcgtcacga tccgcgtggt ctacaaacgg
     1141 acggacctcg ttgacgccgt gctcacgacc gtcgagcgga acctttttga aggggcgatt
     1201 ctcgtcatcg ccgtcctgtt cgccttcctc ggaaacctcc gggccgggct catcgtcgcc
     1261 tcggccatcc ccctttccat gctcttcgcc gtcaccgtga tggagcggat cggcatcgct
     1321 ggaagcctga tgagcctggg tgccatcgat ttcgggctgg tggtcgatag ctccgtggtt
     1381 atggtcgaga attgcgtcag acgcctcgcc catgaccgct cgaatcgaac caagcttgcc
     1441 atcatccgcg ccgccgccat tgaggtgaga aagccgacga tgttcggcga actcatcatc
     1501 atgatcgtgt atcttccgat tctaactctg gagggaatcg aggggaaact cttccggccg
     1561 atggccttga cggtcatctt cgccctcgcg gcgtcgctgg tgctgtccct cactcttatg
     1621 cccgtcctcg cctctctcgg cctgccgcgt cgcatttcgg ataaggaaac gttggttgat
     1681 cgcctcgccc actggctgtt tcagccgatt cttcgctggg gactaaggca tccgctttcg
     1741 accctttgtc tggtcgcaat gctaactgtc ggcacgacca tcctcggtct gcgcctggga
     1801 tcggagtttg tcccccgctt gaatgaaggc tctttggtca tcaacaccat ccggctcgcc
     1861 ggcgttagtc tggaggagtc gctccgctac ggcacgcaca ttgagggttt ccttaagaag
     1921 aggtttcccg atgagatcga ggacatctgg accaggaccg gcagtgccga agtcgcaacc
     1981 gacccgatgg ggatggaatt gtccgacatc ttcatcacct tgagaccccg ctccgggtgg
     2041 acgcgggcga agacgcaaga cgagctggtt tccgcgatgg cccaggtgac gaaagtactt
     2101 cccggcatgc gggccgtcta cacgcagcct atcgaaatgc ggatcaacga gatggtggct
     2161 ggcatccggg ccgatctcgg catcaaactc tatggcgagg atctggaaac gctcaaagcc
     2221 aaggccgaag agatcgaaaa gattgtcaag tctatccctg gtgcggccga tacgacgacc
     2281 gagcaggtga ccgggttgcc ggtgctccga atccatgtcg ataacgaggc cctttcccgc
     2341 ttcggcgtgc ccgcacagca agtgcttgat gccattaagg ccgtcggagg tatcgacgtc
     2401 ggcgagatca tcgagccggg aagacgcttt ccgctggttg tacgtctgcc aatggcgtac
     2461 cgcgaggacc cgcaggccct ggaacagata tcgattccca ccgcgtccgg gcaacgtctg
     2521 ccgctgaccc aactcgtgcg gatcgaggac agcaccggac catcgaccat ccaacgcgat
     2581 tggggcgagc gtcgcataat cgtgcagacg aatgtgaggg gccacgacat cggctcattc
     2641 gtcaaagagg cccaggagcg aatcggccgc gagattgcat ttcccgtcgg ctacacggtc
     2701 gaatggggcg ggcagttcga gcacttgcaa cgggccgagc agcggctcta tgtcgtcgtg
     2761 ccgctggccc tgaccctgat ccttagcctg ctttatctca ccttccgttc gcttcgcgac
     2821 gcggtgatga ttttcagcgg cgtgttattc gcccgcgtcg gcggcatcct cgggctttat
     2881 atcatgggcc tgccgtttac gatctccgcc ggtgtcggtt tcgtcgccct ggccggtgcg
     2941 tcaattctgg aaggccttat ccttgtcagc tccatccgcg accgcctggc ccacggcgtc
     3001 ccgaaacgcg aggccatcga gcaggcccgc cttgccaggc tacggcccgt actcatgacg
     3061 ggcaccgtcg cctccctggg cttcgtgccg atgatgctcg ccaccggcat cggtgccgaa
     3121 gtgcaacggc cgttggcaac cgtcgtcgtc ttcggcatgg tttgtgatac gtttctgacg
     3181 atgctcgcgc tgcccgtgct ctatctcctc ttcgggaaag gaccgctggc ggaaaccgaa
     3241 aacgatcagg aaccgcctga cgaaacgacg atcgaggcga ccgaggggct tcaggccgtg
     3301 aggacttagt ccctcgctgg tcacttgcgg cactgttcgg ttttctcatc gcggcggcca
     3361 gcggcgccct cggggtgccc gctggtgaga tgcgaatccc ggcgctcatg tacccgtttg
     3421 ccgtgccagt aaaagcggcc ggaaccgtta g
//
LOCUS       DATAQL010000618         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4032785, whole genome shotgun sequence.
ACCESSION   DATAQL010000618 DATAQL010000000
VERSION     DATAQL010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4032785"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VHS97_10790"
     CDS             complement(<1..389)
                     /locus_tag="VHS97_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448731.1"
                     /translation="MSERSILLGVTSNCSDAIDGRVFASIPLDRSVLECLKKRTQCFA
                     NAKLIYPQLYETYEFNCLPEWYVTGSSEAYNDQSDQNKLQRLSYDAELVQSEPVDESL
                     SAERDRLDLQENNSLVVPIADLPSSGNL"
     gene            complement(471..1076)
                     /locus_tag="VHS97_10795"
     CDS             complement(471..1076)
                     /locus_tag="VHS97_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448732.1"
                     /translation="METIDEMTLEDAARKAAGNWQSWTCFVWFRDTEVGDPANWSIIY
                     TQNRDSGLLKQSNAEVIAKALNPFTSGEDPDVVFESHSHWAVGHVDGFSIRVYRGADI
                     TDAFRTYHELSERLAEYPILDESDYSEREHDATLENLADACWRLKGQCQLPDGWDSEV
                     YSWLSDHRPGSVENRDDRGGYPSEADLRAAFGALGYERVEV"
     gene            complement(1119..1751)
                     /locus_tag="VHS97_10800"
     CDS             complement(1119..1751)
                     /locus_tag="VHS97_10800"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX3448733.1"
                     /translation="MSKPHDFGLFARLLWLWPLALIAGLCIAGLGLYRAIEDLQSLSI
                     GIKETPPSFRSTGPTVVQLERLQYLVSTRVNVADVLVGESRWLEGSWIIQGDALIGVD
                     MSKAQVREKDPKGQTAVIILPRPTVLSPRVNHGRTKQWDIKSRSWIPLASLLLGDRTT
                     MEKQAMLEAQQLIERAAASEEINETARRFVQGTLAELYRGLGWNVSVQWQ"
     gene            complement(1748..1936)
                     /locus_tag="VHS97_10805"
     CDS             complement(1748..1936)
                     /locus_tag="VHS97_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448734.1"
                     /translation="MIDRLKQIAGRFVELVLVATLALQALVCGCDKEAKWKGISTDSN
                     RNSDGATVRDASMGGRRR"
BASE COUNT          384 a          662 c          649 g          443 t
ORIGIN      
        1 aggttcccgg agctgggtag gtccgcgatg ggaaccacga gtgagttgtt ttcctgaagg
       61 tcgagcctgt cccgctcggc actgagcgac tcgtcaacgg gctcactctg gaccagttcg
      121 gcgtcatatg acagccgctg gagcttgttc tgatcggact ggtcgttata ggcttcgctc
      181 gagccagtta cgtaccattc gggcaggcag ttaaactcgt aggtctcgta gagctgcggg
      241 taaattagct ttgcgttggc gaagcactgg gtccgcttct tgaggcactc gagcacgctg
      301 cggtccaacg ggatggatgc gaaaacgcga ccgtcgatcg cgtcggagca attgctggtg
      361 actcccagca aaatgcttcg ttcggacatg tggaatctcc tttcttgtca cggcagggcg
      421 ccacgctcca agatcgatgc ctggcttgat cgtcgccgtt tcacccggta tcagacttca
      481 actcgctcgt accccaaggc gccaaaggcc gcgcggaggt cggcttctga agggtagcca
      541 cctcggtcgt cgcgattctc gacactgcca ggtcgatggt ccgagagcca cgaatagacc
      601 tcgctgtccc agccgtcagg gagttggcac tggcccttca gccgccagca ggcgtcggcg
      661 aggttctcga gggtcgcatc atgttctcgt tcggagtagt cgctctcgtc aagaatgggg
      721 tactcggcca gccgctcgga gagttcatgg tacgtgcgaa aggcgtccgt gatgtcggcg
      781 ccacggtaaa cccggatgct gaacccatcc acgtgcccga ccgcccagtg agaatgactc
      841 tcgaagacaa cgtcggggtc ttcgcccgag gtgaaggggt tcaaggcttt ggcaatgact
      901 tccgcgttac tctgtttgag caagccggag tccctgttct gcgtgtagat gatggaccag
      961 ttcgccgggt cgccgacctc agtgtcccga aaccaaacga aacacgtcca agactgccag
     1021 ttcccggccg ctttgcgagc ggcgtcctcg agggtcattt cgtcgatcgt ttccatttaa
     1081 tgcctccgtg gggaggggcg ttggtttgcg tgccttcact actgccactg gaccgagacg
     1141 ttccagccta agccgcgata taactcggcg agcgtgcctt gaacgaagcg acgtgccgtc
     1201 tcgttgatct cttccgaagc cgccgcgcgt tcgatcaatt gttgggcctc gagcatcgcc
     1261 tgcttctcca tggtggtccg gtcgccgagc aacaggcttg ccagcggaat ccagctccgg
     1321 gacttgatgt cccactgctt ggtccgtccg tggttcacgc gaggcgagag caccgtcggc
     1381 ctgggcaaga tgatcaccgc ggtttgcccc ttggggtctt tctctctgac ctgcgccttg
     1441 gacatatcca cgccgatcag cgcgtccccc tggatgatcc aggacccctc cagccagcgg
     1501 gattctccaa cgagcacgtc ggcgacgttc acccgcgtgg agacgaggta ttgcagtctc
     1561 tcaagctgca cgaccgtcgg accggtggat cgaaacgacg gcggcgtctc ctttatgccg
     1621 atgctcaggc tctgaaggtc ctcgattgct cggtagaggc caaggcccgc gatacacagc
     1681 ccggcaatca gagccagggg ccagagccac agcaaccgcg cgaataaccc gaagtcatgg
     1741 ggtttgctca tcggcgcctc ccccccatgg aagcgtcgcg aacggtggcc ccgtcgctgt
     1801 tcctattgct gtccgtcgag attcccttcc actttgcttc cttgtcgcac ccgcacacca
     1861 gcgcctgcaa ggcgagggtg gcgaccaaga cgagctcgac gaaccggccg gcgatctgct
     1921 tgagtcgatc gatcatggac gaagctcctg gggtgtaccg aggaatgaaa aacggcgggt
     1981 cgccccgccg ccgatgaagg atctttcttg tcaacccgga caatgccagg tcgcgatgtg
     2041 cgctcagttg agcgaggcgt cgccgcgatt cacctgccgc ccggccttgc ctgaacccgc
     2101 gggtgcccgc gagtagatcc ctggccgatc tgccgaca
//
LOCUS       DATAQL010000619         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5101318, whole genome shotgun sequence.
ACCESSION   DATAQL010000619 DATAQL010000000
VERSION     DATAQL010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5101318"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..679
                     /locus_tag="VHS97_10810"
     CDS             194..679
                     /locus_tag="VHS97_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX3448735.1"
                     /translation="MFDVREAPVDVELIARGLEAGVVYRPFEGDVSGMVYRNRREKVI
                     GVNSSHPQNRQRFTVAHEIGHLVLHKGQPVFVDSFEGRINWRDGASAKEESEANAFAA
                     ELLMPRALVLGEVDRAVGEEDLSAATLTERMARDFKVSQQSMRYRLENLGVLDPGGLL
                     E"
     gene            complement(1344..1748)
                     /locus_tag="VHS97_10815"
     CDS             complement(1344..1748)
                     /locus_tag="VHS97_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448736.1"
                     /translation="MRSRRRSIVSLVAVLAASVIATGCSGSSASTSRSEAAAVAHAER
                     AFIKSWDEATAKATDRCEATANPEHCFALAFRPGAKTVVSHFNDAIEAVMADGVGSEC
                     TAALEEALVEPAQIPSFPGPAAAACRAESRDD"
     gene            complement(1748..1933)
                     /locus_tag="VHS97_10820"
     CDS             complement(1748..1933)
                     /locus_tag="VHS97_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448737.1"
                     /translation="MRSRIAGKLVALVLFSLLGLAVVGGVDALGGQPRRADRGIQTIL
                     TDPAFAPMSLKLAVDGE"
BASE COUNT          372 a          703 c          680 g          361 t
ORIGIN      
        1 tcctccccga ccgccaggta gatctcgagg agttggtctc cgagggcgac cgccagaagc
       61 ttccggcgga ggagtcccac cgcctgttca tggcgagcgt ccttcgcgat aatgcgccgg
      121 tgaggaaaac gcgggacggc gacggcggtt gaccgcctcg agggcagaac gagaggcgac
      181 caaggtgctg gagatgttcg acgtccggga ggcgccggtg gatgtcgagc tgatcgcccg
      241 tgggctcgag gcaggggtcg tctaccggcc cttcgaaggc gacgtctcgg ggatggtcta
      301 tcgaaatcgc cgggagaagg tgatcggagt caactcgagc cacccccaga accgccagcg
      361 gttcaccgtc gcccacgaga tcggccatct cgtcctccat aaaggccagc cggtcttcgt
      421 cgacagcttc gagggccgga tcaactggag agacggcgcc tcggccaagg aggagtccga
      481 ggcaaacgcg ttcgccgccg agctcctgat gccgcgggcc ttggtgctag gcgaggtcga
      541 ccgggcggtc ggcgaggagg acctctccgc cgccactctg accgagcgca tggcacgcga
      601 cttcaaggtc agccaacagt cgatgcgcta ccgcctggag aacctcggcg tcctcgatcc
      661 cggcggcctg ctcgaataga tccgcgtcac gggcgccacg ctccgtccca actcgcgggc
      721 gaggcgcctg atccgatcgg cctcagcgga cccgtacacg ccctctgccg ctggggcgac
      781 gcggtgtggc gcgaaggggt gggcgcaagg tccaggtccc cggaaaaatc tgtgtatccc
      841 cactgaagcg tctctcttga ggccaccagg ccgacgaccc cgaactaggt cacggacctg
      901 tccatgcgac cgacggcaga atgcggccta tcgcgaagga agaagctgct cgagcggtga
      961 cgtcctcaat ctcgaccatg tcaacacggt ggccgagggc ttcatcggtg agcgcaccac
     1021 cacccctcgc gcggagaagc tgcacccctt gagccgtact ctggattttg ccaccggctg
     1081 cgggcgagcc tccggggccc tcaggcagat gattcgaacc gcaccccgcc cggattgggc
     1141 actaatgcga gttcgtgccc gaatcgccgc atatccacgt gtatatccgc gcatatccgc
     1201 cggtatatcg cccggtctgg cgcagtacac agtccggttt ctacctgcaa atcgggggtt
     1261 tgagaaactg gaccgcgcta accgcgagcc tggatcgcac ccaggaggtc ggcggttcga
     1321 atccgcctag ccagccatcg cgtctagtcg tcgcggctct cggcccggca ggcagcggcg
     1381 gctgggcccg ggaacgacgg gatctgggcg ggttcgacca gagcttcttc gagcgctgcc
     1441 gtgcattcgc tgccgacccc gtccgccatc accgcttcga tcgcgtcgtt gaaatgggag
     1501 acgaccgtct tcgctcccgg cctgaatgcc agggcaaagc agtgctctgg atttgcggtg
     1561 gcttcgcatc ggtccgtggc tttggcggtg gcctcgtccc aggacttgat gaatgcccgt
     1621 tcggcgtgcg ccacggctgc ggcctcggac cgactggtcg aggccgaaga accgctgcat
     1681 ccggttgcga tcaccgaagc cgccaagacg gcgacgagac tgacgatcga tctacgtcgg
     1741 cttctcatca ttctccgtcg actgcgagct tcaggctcat cggggcgaag gcgggatcgg
     1801 tgaggatcgt ctggattccc cggtctgccc gccgtggctg gcccccaagg gcgtcgacac
     1861 cgccaacaac cgcgagaccc aggagggaga acaaaaccaa ggcgaccagt tttccagcga
     1921 tccgactgcg catctgtgcc ttcccgtcgg actcgcccgt gcctgatcct tcggccaatt
     1981 cgggagtcgt gcgtccttat gccgacctaa gttagcgtct tccgttcacc gctctgcacc
     2041 acgataggcg cgagccgccc gaacacgtga tccatcagca tcctggcctt gcgctcatgt
     2101 tcggacgtga caacga
//
LOCUS       DATAQL010000620         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1589457, whole genome shotgun sequence.
ACCESSION   DATAQL010000620 DATAQL010000000
VERSION     DATAQL010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589457"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1789
                     /locus_tag="VHS97_10825"
     CDS             371..1789
                     /locus_tag="VHS97_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3448738.1"
                     /translation="MSAGVTQRSTWELAAESIVVKLRWFGVVMGYILVQTRTGLHDPW
                     AVRAFLALGAGFAALDLAFWWMGEVFLKRWPVFVSLMESVFIALLCYHDSGLSSPFRW
                     YYLLSLICCSIRYRSAVAWWTVAFHSLSLLSLALVLGAPSFWNSSLPLTLTIMVWVTW
                     ACSSLAGSLHEAGEALEHLNAELDRSRANLERRVEERTEALRASQARVIQQEKMAAFG
                     LLAAGIAHEVGNPLAALSSLVQILKRRGPDPYTAEKLDLAARQLGRIERTIRELIDFS
                     RPASTAVSRVRLREIVDEALGIAKYYQRTKARTITTDVSPGLPSVTGVRDYLTQVVLN
                     LVLNAVDATDDQGHIEVAARVEDGWLVLTVADDGRGISIADRCRLFQPFFTTKAHGTG
                     LGLFISRQILEDYAGTLSVRSEPGEGSTFFVRLPLESARPPEPVAVDHSEFRMEPVLS
                     EPRAPEPAALIAAATTLGDEPS"
     gene            1786..3201
                     /locus_tag="VHS97_10830"
     CDS             1786..3201
                     /locus_tag="VHS97_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3448739.1"
                     /translation="MKSGALALGNDDDRRAESDGPRGRILIVDDEEVIASTLKEFLQG
                     ESFEVATAHDLNSAIAQVESFDPEIVLCDVQLPGADGITVLNRVLQISPDTLFIMITA
                     YATVENAVSAFQRGAQDYLIKPVLFEDLLAKLGRLIRFRRLLQENQALRRQLHRRAEL
                     DELVGESASIQAVKKLMRKVGPTQTTVLITGESGTGKELVAQGLHALGLGRNEMFLAV
                     NCAAIPHDLLENQLFGHVRGAFTGADRDHAGLFVAAGRGTVFLDEIGELARSTQAKLL
                     RAIENKEVLPVGATRPISFQARLLTATNKDLAAEVAADRFRADLYYRLNVVAIHLPPL
                     RDRREDIPELVSVLLAKHAKSLTKRVVGVDNATIRGLMSAPWVGNVRELDNALERAMI
                     LSEGPILTPDDFPSGLIVETDTDARGDNLRMAMRDYETHHIQRVLRECGDDKREAARR
                     LGMGLSSLYRKLDELGIRLVS"
BASE COUNT          647 a         1005 c         1067 g          716 t
ORIGIN      
        1 ccgtcgtcat cttcaagatg tcttgccgga tcgacttggc tcgcgcaagc aaagcgtcaa
       61 gattattggc cattgctgtt ccctcggtga tgtggcgagc ccatgagtga ccacgcgtgc
      121 gtggatcttt cggtttccgc cgacggcagc caagccatgt tctaaattag actaatttcc
      181 gcaaattgag ttccaaaatc ttgccgattc tgccgtaact cgctcgtttc cgaacgactc
      241 ggcgctgagt tcattcgcgc cccgcgtcta taatcgaaag gtaatgaggc gaccgaaaac
      301 cgaaaactgc aagcgggtac gtgacacgga ttcggtgggg cattgagaca gcacgctgga
      361 gattgaacgg atgagtgcag gagtgaccca gcggtcgacg tgggaactgg cggccgagtc
      421 gatcgtggtc aagctgcgct ggtttggcgt cgtgatgggt tacatcctgg tacagacccg
      481 taccggactg catgacccgt gggcggtgcg tgcatttctg gctctgggag cgggctttgc
      541 cgccctcgac ctggcgttct ggtggatggg tgaggtgttc ctcaagcgct ggccggtctt
      601 cgtgtcgctt atggagtcgg tatttatcgc gctcctctgc tatcacgact ccggtttgag
      661 cagtcccttc cggtggtatt acttgctctc gctcatttgt tgctcgatac gttatcgatc
      721 ggcagtcgct tggtggaccg tcgcgtttca cagcctcagc ctgttgagct tggcactcgt
      781 gctgggggcc ccttcgttct ggaattcgag cttaccgttg acactgacca tcatggtctg
      841 ggtgacatgg gcttgctcgt cgctggcagg gtcgctgcat gaagcggggg aagcgctcga
      901 gcatctgaac gccgagctcg acaggtcccg cgccaacctg gaacgccgcg tcgaggaacg
      961 gacagaggcc ctgcgcgcat cgcaggcgcg ggtaattcag caggagaaaa tggcggcgtt
     1021 cggattgctg gcggcgggga tcgcgcacga agtcggcaat cccctggccg cactcagttc
     1081 cctggtgcaa atcctcaagc gacgcgggcc tgatccctac accgccgaga agctcgacct
     1141 ggcggcacgc cagctcgggc ggatcgagcg cacgatccga gagttgatcg acttttcgcg
     1201 gccggcctca acggcagtca gccgcgtgcg tctccgcgag atcgtcgatg aagcgctcgg
     1261 catcgccaag tactaccagc gcaccaaggc gcgcacgatc acgaccgacg tttcccccgg
     1321 cctcccttcg gtcacgggcg tccgtgacta cctgacgcag gtggtcttga acctcgtctt
     1381 gaacgccgtc gacgccaccg acgaccaagg ccacatcgaa gtcgcggcaa gggtggaaga
     1441 cggttggctc gtcttgaccg tcgccgatga tggccggggc atctcgatcg cagaccggtg
     1501 ccggctcttc cagcctttct tcaccacgaa ggcgcacggg actggtctgg gcctctttat
     1561 cagccgccag atcctggaag actacgccgg caccctttcc gttcgatccg aaccgggtga
     1621 gggatctacc tttttcgttc gactcccgct ggaaagcgcc cggccaccgg agccggtagc
     1681 ggtcgatcac tcggaatttc gcatggaacc cgtgctgtcg gagccgcgcg cgcccgagcc
     1741 ggcggcgctc atcgccgctg caacgaccct gggagacgag ccttcatgaa gtcgggagcg
     1801 ctggctctgg gcaacgatga cgatcggcgt gccgaatcag atgggccgcg gggacggatc
     1861 ttgatcgtcg acgatgagga ggtgattgcc tcgaccctca aggagtttct tcagggagag
     1921 agcttcgagg tggcaacggc ccacgatcta aattcggcaa tcgcgcaagt cgagtctttc
     1981 gatccggaaa tcgtgctgtg cgacgtccag ctccccggcg ccgacgggat taccgtcttg
     2041 aaccgcgtgc ttcagatcag tccggacacg cttttcatca tgatcaccgc gtacgcgacc
     2101 gtcgaaaatg ccgtctccgc atttcagcgc ggcgcgcaag actatctcat aaaaccggtt
     2161 ctcttcgaag acttgctcgc caagctcggt cgcctcattc gctttcgtcg cttgctccag
     2221 gagaatcagg cactgaggcg ccagttgcat cgaagggccg agctcgacga gctggtgggc
     2281 gaaagtgcgt cgatccaggc tgtcaagaag ctgatgcgca aggtggggcc gacgcaaacg
     2341 acggtattga tcaccggcga gtcgggcacc ggcaaagagc tggtggccca aggcctgcat
     2401 gccctgggcc tcggtcgaaa tgagatgttt ctcgccgtca actgtgcggc gattccccat
     2461 gatctcttgg aaaatcagct ctttggccac gttcgaggcg ccttcacggg cgcggatcgc
     2521 gaccacgcgg ggcttttcgt tgcggccggg cgggggactg tttttctcga cgagatcggc
     2581 gagcttgcac ggtcgacgca ggccaagctg ctgcgggcga tcgaaaacaa ggaagtattg
     2641 ccggtggggg caacccggcc catttcgttt caagcaaggc tgctgacggc gaccaacaaa
     2701 gacctcgccg ccgaggtcgc cgccgacagg tttcgggccg atctgtacta ccgtttgaat
     2761 gtcgtggcca tccatttacc accgctgcgc gatcgtcgcg aagatatccc cgagcttgtg
     2821 tcggtgttac tggcgaagca cgccaagagc ctgaccaagc gagtcgtggg cgtcgacaat
     2881 gcgaccattc gcggattgat gtcggcccct tgggtcggta acgtgcgcga gctcgataac
     2941 gcgctcgaac gcgcgatgat cttgagcgag ggaccgatac tcacgcccga cgattttccg
     3001 tcgggcctga tcgtcgagac cgacacagac gcgcggggtg ataatctgcg catggcaatg
     3061 cgcgactacg aaacgcacca cattcagagg gtgttgcggg aatgtggcga cgataagcgc
     3121 gaagcggctc gccgactggg aatggggctg tcgtcgctgt accgcaaact cgatgagctt
     3181 ggaattcgtt tggtctcatg accgatgacg gccagccagt tctccttcag cgagtaagcc
     3241 gtgatttcac tgcgtcagta gtcgagtcag ccggcgcgat ataattccac tattgaacta
     3301 tttgactttc taactgactg ggtctatcat gtcctcgagc gaatcgaagc aagagtcgga
     3361 tgcctggaac attctggcgc atctctggca gttgacccaa tcagttttgg atgagtcggc
     3421 acctgcattg gaggc
//
LOCUS       DATAQL010000621         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1132577, whole genome shotgun sequence.
ACCESSION   DATAQL010000621 DATAQL010000000
VERSION     DATAQL010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132577"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VHS97_10835"
     CDS             complement(<1..769)
                     /locus_tag="VHS97_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3448740.1"
                     /translation="MSFYLATLAVYMVIYALGAWALNLQFGLGGIVNFAFIVFESIGA
                     YAAAITSLGRTGALTGQTYFWGASWPFPLPLIAGAAAGGLLALIVGPATMRKMRRDYQ
                     AAAMLVFALIADQVITNDTHLFNGAAGLAGVQQPLSSSLGLSLGFYDWLFVIFAAMIG
                     VAAYLMFRRISLSPLGRTLRALRDDEEAASAVGKNAWAMRMTVFVVGGAVAGLAGALL
                     IDFIGAWSPAAWSYQETFLLLVAIILGGVGNDRGVFLG"
     gene            complement(782..1669)
                     /locus_tag="VHS97_10840"
     CDS             complement(782..1669)
                     /locus_tag="VHS97_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3448741.1"
                     /translation="MNVIVAAISFGLVSGAILSVSAIGFTLQFGMTNILNLAYGSVMT
                     LGALITYLIGNMGYSQWLGAAAGTIGAGILSLALGRTLIKAFARRGSRVFEMAMVTVA
                     ASLVIDYALASITHSNILQFNFPTGREHRLLGMAFSTTELIIMGIVIVAVAALEFALH
                     WTRMGVALRATAANPALARASGINSTQIVNITWVVSGCFAGLGGVTLALSYSSVSFTI
                     GTSYLPYILAVVVLAGIGSIGYAVLIAIALSVVIQIAGALGAANYDVAIALCLLFVTL
                     LIRPQGLFGQLFDKQEIVA"
     gene            complement(2010..>2911)
                     /locus_tag="VHS97_10845"
     CDS             complement(2010..>2911)
                     /locus_tag="VHS97_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3448742.1"
                     /translation="LSSAGESQFDRTNLKYFWRMFPPDSENGIAMALTAKSLGITRVA
                     AVFGSDQGSQGDLPGVLAGIKAAGLDLVAEQSLTPDQPSYQAQVARVLAGKPQAIFTE
                     ADPTSATTFFGEMVHQPGGSGVRIIGSQATVVPSWTTPVQGAVGKSLFQSLYLGVSAQ
                     SATANPATALFNSALNASGASVPNPKQYIGNPYAMLGYDSCIAAALAAVDAHSYRSPV
                     YNDFIPQVTNPGPGKTVVYSYAEGVRLLKQGKKIQYVGAAGPIDFNKYHNSSGGQVAE
                     NLTSSGTLSVIKVISFQQISRLG"
BASE COUNT          624 a          918 c          829 g          540 t
ORIGIN      
        1 ccccgagaaa aacaccgcga tcattgccta ctcctcccag aattattgcc acgaggagaa
       61 ggaaagtctc ttgatacgac caggcggccg gcgaccaggc tccgatgaag tcgatcagca
      121 aggcaccagc caggcctgcc acagcacctc ctaccacgaa gacagtcatc cgcatggccc
      181 aggcgttctt tccgacggcc gacgcggcct cctcatcgtc gcgcagagcg cgcaacgtgc
      241 gcccgagggg ggacaggctt atccgtcgga acatcaggta tgccgcgacc cctatcatcg
      301 ctgcgaatat cacgaacagc cagtcgtaaa atcccagcga cagaccaagg ctgctgctca
      361 gcggctgttg cacgcctgcc aggcctgcag cgccgttgaa gagatgagtg tcgttggtaa
      421 tgacctgatc cgcaatgagc gcaaaaacta gcatcgccgc ggcctgatag tcgcgacgca
      481 tctttcgcat ggtcgcaggg ccgacgatca gcgcgagcag ccccccagcg gccgcacccg
      541 cgatcaacgg caacgggaac ggccaactcg ccccccagaa atacgtctgg ccggtcagcg
      601 ctcccgtcct gcccagcgac gtaatggcag ccgcgtacgc gccgatcgac tcgaacacga
      661 tgaaggcgaa gtttacaatt ccgccaagac cgaactgaag gttgagcgcc cacgcgccga
      721 gcgcatagat caccatgtag acggccagcg tggccaggta aaaggacact gcagctccga
      781 ttcaggcgac tatttcctgc ttatcaaata gctggccgaa caagccttgc ggcctgatca
      841 gcaaggttac aaagagcagg cacagcgcaa tggctacgtc gtaattcgcc gcgccgagtg
      901 cacctgctat ctgaatgacc acgctcagag cgattgcaat gagcactgcg taaccgatcg
      961 atccgatgcc ggcgagcacc acgaccgcca gtatgtaggg aagataactg gtgccgatcg
     1021 tgaacgatac gctgctatag ctcagcgcca gggtgacgcc gcctagacct gcaaagcacc
     1081 ctgacaccac ccatgtgata ttcacgatct gggtagaatt aattcccgaa gcgcgagcca
     1141 gggccgggtt tgccgcggta gcccgcaaag ccacgcccat gcgcgtccag tgcagtgcga
     1201 actcgagagc agcaactgcc acgatcacta tccccatgat tatgagttcc gtggtcgaaa
     1261 acgccattcc gagaagtctg tgttctctgc ccgtggggaa gttgaactgg agaatgttgc
     1321 tgtgcgtgat ggaggccaaa gcgtagtcga tgactagcga ggcggcaacg gtcaccattg
     1381 ccatctcgaa gacccgggat ccacgacgag cgaaagcctt gatgagagtt ctcccaaggg
     1441 caagcgacag gattccggcg ccgattgtgc cagccgctgc cccaagccac tgcgagtaac
     1501 ccatgttacc gatcaagtac gtaatcagcg cgcccagcgt catgactgat ccgtacgcca
     1561 agttcagaat gttggtcatg ccgaactgca atgtgaagcc gattgcgctc acactcagta
     1621 tggctccgga aaccaagccg aagctaatgg cggccacaat aacgttcaca gggctcgtcc
     1681 tcgtttcgac tggccgattc cgggagcctg gtggtgcccg tccgggcggc attcgccgag
     1741 ctttgctctg gccaccaccc ctctggtgag caccctaagc cgcccttctt tggcgccaca
     1801 cgttctgcct ttggaaagca gcggctcccg ccgaccatct gcactcggct tattcatccg
     1861 ccgctgcgtt cgcgttctgg aacatttccg tgatcaactt ccagaacctc cgccggatcg
     1921 ttcccgtcca agccgactcg ggccggtgtt cgcatcaccg gcccgtgtcc caggacccga
     1981 ccggtcgcgg ccgtcatacg tcgctccatt cagcccagcc ttgaaatctg ctggaaggaa
     2041 atgaccttta tcacgctcag cgtgccggac gacgtgaggt tctccgccac ttggccgccc
     2101 gacgagttgt ggtatttgtt gaaatcaatc gggccggcgg ctccgacgta ctgaatcttc
     2161 tttccttgct tcaggagcct cacgccctcg gcataggagt aaaccacggt ctttcctggg
     2221 ccgggattag tgacctgagg aatgaaatcg ttgtataccg gcgaccgata ggaatgcgca
     2281 tccaccgcgg ccagggcagc tgcgatgcaa gagtcataac ccagcatcgc atacgggttg
     2341 ccgatatact gcttcgggtt cgggacactc gccccagacg cgttcagtgc actattgaag
     2401 agcgctgtgg ccggattggc cgtcgctgac tgtgccgaga cgcccaggta caggctctgg
     2461 aacaggctct ttccgaccgc cccctgaact ggagtcgtcc aactcggcac gaccgtcgcc
     2521 tgagaaccga tgattcgcac gcccgaaccc ccgggctgat gtaccatctc gccgaagaat
     2581 gtcgtagcgg acgttggatc ggcttccgtg aatatggcct ggggcttccc tgcgagaaca
     2641 cgggcgacct gcgcctggta ggagggctgg tctggcgtga gactctgctc tgcgacaagg
     2701 tctagtccgg ccgccttgat accggccagg acgccgggaa ggtctccctg agatccctgg
     2761 tcggagccga atacggctgc caccctggta attccgagcg acttggccgt caacgccata
     2821 gcgattccgt tctcggagtc aggcgggaac atacgccaga aatatttgag gttggtccgg
     2881 tcgaactggc tctcgccggc ggaggatagc a
//
LOCUS       DATAQL010000622         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_825739, whole genome shotgun sequence.
ACCESSION   DATAQL010000622 DATAQL010000000
VERSION     DATAQL010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825739"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VHS97_10850"
     CDS             complement(<1..273)
                     /locus_tag="VHS97_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448743.1"
                     /translation="MDRDHSTGSSARPSWRVWLFRLLLSLLLAVGYAFCYFIIPKIEV
                     YWTTRVPDVPRWEIVLSSCYHLFIAHCPAILLVLTAFILLRRWFCAL"
     gene            complement(668..2914)
                     /locus_tag="VHS97_10855"
     CDS             complement(668..2914)
                     /locus_tag="VHS97_10855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3448744.1"
                     /translation="MTFRSTLDAALAAADLHEIDAICDRFESAWRAGRRPDLASFLSE
                     APPGGLALLFRDLLNLDLEFRVKSGETLDAQSYIQKFPELADHVDAAFALRRDNPSLT
                     RLGQRTDWAGGSTVSSLNERDPAIEASPWDQFTMGAGATPVVRGYEILGELGRGGMGI
                     VFKAHQVALNRAVALKMIKSGGFASEAELLRFQNEAEAVARLDHPHIVPIYEVGRHLG
                     RHYFSMKLIAGKSLDRRLGDFIADFRQGARVTATVARAVHHAHQRGILHRDLKPANIL
                     LDEQGEPHVTDFGLARPIEGNGELSESGTPVGTPSYMAPEQASGDKAALSTATDVYGL
                     GTILYALLAGRAPFAGTTVHETLDKVRNSSPELPSRVNKRVPRDLEIICLKCLEKEPN
                     RRYPSALALAEDLERWLDGMPILARPVGSLTRAVMWCRRNKALASLAALLMLALVGGL
                     AGVTWKWREADHERAKNEAVIELLNQGLLAQASLELDPLEKNLSVRELLDRTAARLGG
                     WFQDQPETEAKIRETIGGAYLSLARYEPAEKHLRAAAQLDTKLYGPKHRDTLRANNLL
                     ASLFDQTNRGAEAEQLLRQNLDDCRIQLGRDDRVTLDAAERLGTILWHVGKGDEAEAV
                     LRTNVNDRSRVLTPEHPETLRSTYLLSRLLRERHQFPDAEHFAYLFAQSVKCSLGANH
                     PDNVVALTNQADVLRDQGKLAAAEPIYRQAAVEAERIRGAEDPSTVAAKNDHARVLSE
                     MAQRPAGQ"
     gene            complement(2911..3528)
                     /locus_tag="VHS97_10860"
     CDS             complement(2911..3528)
                     /locus_tag="VHS97_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3448745.1"
                     /translation="MSDGESVTRWLQGVKAGDSADIERLWNRYFQRLVRLAGAKLPGH
                     CRRAFDEEDVAISAFQSFCDRAGRGEFPQLNGRDDLWRLLAMLTVRKAAVTMRHQTRQ
                     KRGGGRVLGESAFGVGARDGESETCSGIAMILSREPTPDDAARFTDAFDKLFARLADQ
                     TLRTIALRKLKGDTVEEVAASLGTSTRTIDRKLRLIRAIWEEAAG"
BASE COUNT          710 a         1160 c         1147 g          697 t
ORIGIN      
        1 caacgcgcaa aaccagcggc gaagcaggat gaaagctgtc aaaaccagca gaatcgcggg
       61 acaatgcgcg atgaataaat gataacatga actcagcacg atctcccatc gtggaacgtc
      121 cggcacccgg gttgtccagt atacttcaat cttgggtatg atgaagtaac aaaatgcata
      181 tcccactgcc agcagaagcg acaacaggag gcggaacaac cagacccgcc aggacgggcg
      241 ggccgaactg ccagtcgaat ggtctcggtc catgttgcac tcggctctct ccagttgacc
      301 cccgccatag gccgaagaaa agtcgccctt tcggcgctgc tgcattcctc ccgttttagt
      361 tgcaccactt cgtcgagtac aactttcccc cccttgtgaa ggagggttgg gggggtggtc
      421 agcgagctac cagacacagt gagacccaag ggggggtggt cagcgagcta ccagacacag
      481 tgtggtcaac gtgaattgag gtggtcccgg tgctatcaat tacataatat tcatagctaa
      541 tgagtgaaag cagcttgatt tcttaatgaa atctctggaa gaactgcgcc tgattgctca
      601 ctgaccaccc ccctgccccc cgttcacaag gcggggaaag gaaaggcatc cgccgcgcaa
      661 cgtccgatca ctgtcccgcc gggcgttgag ccatctcgct cagcacacgc gcatgatcgt
      721 tctttgccgc caccgtggaa gggtcctccg ccccgcggat gcgctcggct tccactgccg
      781 cctggcgata aatcggctcg gcggcggcga gctttccttg atcgcgcaac acgtccgcct
      841 gattcgtgag tgccacgacg ttatcggggt ggttggcacc cagcgagcac ttaacgcttt
      901 gtgcgaaaag ataggcgaag tgttcggcgt cgggaaactg gtggcgctcc cgcaagagcc
      961 ggctcaagag gtaggtcgag cgtagtgtct cagggtgctc gggcgtgagc acgcgggagc
     1021 ggtcattcac gtttgtccgc aaaacggcct cggcttcatc ccctttgccg acgtgccaga
     1081 ggatggtccc aaggcgttcg gccgcgtcga gggtgacgcg gtcgtcgcga ccgagctgga
     1141 tgcggcagtc gtccaggttt tgccgcaaaa gttgctcggc ttccgcgccc cggttggtct
     1201 ggtcgaaaag cgaagcgagc agattgttgg cccgcagcgt atcgcgatgc ttggggccat
     1261 agagcttggt gtcgagctgg gccgcggcgc ggagatgttt ctcggccggt tcgtaccggg
     1321 ccaacgagag atacgcaccg ccgatcgtct cgcggatctt ggcctcggtc tcgggctggt
     1381 cttgaaacca gccgccgagg cgcgcggccg tacggtcgag cagctcacgg acgctcaggt
     1441 ttttttccag cgggtcaagc tcgagcgaag cttgtgccag cagcccctga ttcaagagct
     1501 cgatgacagc ttcgttcttg gcgcgttcgt gatcggcctc gcgccatttc caggtgacgc
     1561 cggccaggcc cccgaccagg gccagcatca agagcgccgc cagcgaggcc agcgctttgt
     1621 tgcgccggca ccacatcacg gcgcgggtca gcgagcctac cggccgcgcc aagatcggca
     1681 ttccgtcgag ccagcgctca aggtcttcgg ccagtgccag cgcgctggga taacgacggt
     1741 tgggctcttt ttccaggcac ttgaggcaga tgatctccag gtcgcgcggc acccgtttgt
     1801 tgacccgaga cgggagctcg ggcgaactgt tgcgcacttt gtcgagcgtc tcgtgcaccg
     1861 tggtgccggc aaatggcgcc cggccagcca aaagcgcgta gaggatcgtt cccaggccgt
     1921 agacatcggt cgctgtcgac agggcggctt tatctccgct ggcctgctcg ggagccatgt
     1981 acgacggagt acccaccggt gtgcccgatt cggacagctc gccgttgccc tcgatcggtc
     2041 gggccaaccc aaaatcggtg acatgaggtt cgccttgttc gtccagcaag atgttggccg
     2101 gcttcaagtc gcgatgtaga atgccgcgct ggtgggcatg gtgcaccgcc cgggcgacag
     2161 tcgccgtaac ccgggcccct tgccgaaaat cagcgatgaa gtcgccgagc cgccgatcaa
     2221 gacttttgcc cgcgatcagc ttcatgctga agtaatgccg ccccaagtgc cggccgacct
     2281 cgtaaattgg cacgatgtgg gggtggtcga gccgcgccac agcctcagct tcattttgaa
     2341 accgcagcag ctcggcttcg gaagcaaagc cgcccgactt gatcatcttg agcgcgacgg
     2401 cgcggttgag cgcgacctgg tgggccttga agacgatgcc catccctccc cgccccagct
     2461 cgccgagaat ttcgtatccc cgcacgactg gcgtcgctcc tgcgcccatc gtgaattggt
     2521 cccacgggga tgcttcaatg gcaggatctc tctcattgag tgatgagacg gtcgagccgc
     2581 cggcccaatc cgttcgttga ccgagccgcg tcaggctcgg gttgtcgcgc cgtagcgcga
     2641 atgcggcatc gacgtgatcg gccagctcgg gaaacttctg gatatacgac tgcgcgtcga
     2701 gcgtctcgcc gctcttcacg cggaattcca gatcgaggtt caaaagatcg cgaaacagca
     2761 aggcgaggcc cccgggtggt gcctctgaca aaaacgacgc cagatccggg cggcgtcccg
     2821 cgcgccaggc ggactcgaag cgatcgcaga tcgcgtcgat ctcgtgcaga tcggcggcgg
     2881 cgagcgcggc gtccagtgtc gagcgaaagg tcatcctgca gcttcctccc agatggcacg
     2941 tatcagccga agcttgcgat cgatggtccg ggtcgacgtt cccagcgacg cagcgacctc
     3001 ttctacagta tcacctttga gcttccggag ggcgatagtt cttagggtct gatccgcgag
     3061 ccgcgcgaac agtttgtcaa acgcgtcggt gaaccgagcc gcgtcatccg gagtgggctc
     3121 tcggctcaag atcatggcga tgccgctaca tgtctcggac tctccgtccc gcgcgccgac
     3181 gccgaaggcc gactcgccca gcactcggcc gccgccgcgc ttctgacggg tctggtggcg
     3241 catcgtgacg gccgctttgc gcaccgtcaa catggccagc agccgccaaa ggtcgtcccg
     3301 cccgttcaac tggggaaatt cgccccggcc cgcgcgatcg caaaagctct ggaacgcgct
     3361 gatcgccacg tcttcttcgt cgaaagcacg gcggcaatgg ccgggtagct tggcacctgc
     3421 cagccggacc agtcgctgaa aatagcggtt ccagagccgc tcgatgtcgg cgctgtctcc
     3481 cgccttgact ccctgcagcc aacgcgtcac cgattcgccg tccgacacga caaagcctcc
     3541 cggtgcgcag gaagatttcg cttcgacaca attcactatc gccgaaacct agtggaccgg
     3601 acatgaaatg gcgagaaaat tttgggggaa gtcaatgcac tactactcgg agagcacgcg
     3661 aaccgcaatt cggtcaaccg tttcggcggg gtcgtgagac gctcgccgaa cgtg
//
LOCUS       DATAQL010000623         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3728374, whole genome shotgun sequence.
ACCESSION   DATAQL010000623 DATAQL010000000
VERSION     DATAQL010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3728374"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VHS97_10865"
     CDS             complement(<1..226)
                     /locus_tag="VHS97_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX3448746.1"
                     /translation="MRGAVRGYEIIPGFAIAAHGPVRSVKLFSRVPWHRVQRLALDAG
                     SRTSQVLARIWLETRHAVRPKQVEELPLGVS"
     gene            complement(201..401)
                     /locus_tag="VHS97_10870"
     CDS             complement(201..401)
                     /locus_tag="VHS97_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX3448747.1"
                     /translation="MAPPIRVGAVSYLNAKPLYYGLCEHFPQIRLSMEVPSRLAEQLS
                     DGELDVALNPVGRVFAWCCPGL"
     gene            complement(689..1021)
                     /locus_tag="VHS97_10875"
     CDS             complement(689..1021)
                     /locus_tag="VHS97_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448748.1"
                     /translation="MASAFQNSRARLRCASVAVAAERYRLARSIWPSALESLIPDFLA
                     KVATDPFGGEPLHLRRFADRIVIYSVAEDGRDDGGDAELTSLLATDRDVSFRLWDPAR
                     RHRPAVNP"
     gene            complement(1076..>1608)
                     /locus_tag="VHS97_10880"
     CDS             complement(1076..>1608)
                     /locus_tag="VHS97_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448749.1"
                     /translation="TQLRHPATARDVVNLLAYDVLLRSQKQETDEALLTCHALLNVGR
                     SIGDEPTAVSQRLRIDIRMTTCQQVEFALAHGRASEAVLARLQHALDAEEDEPLLVSA
                     IKGERALMDRLLTSVASGEFTPRQVRNTTFFSTILHVFRNSTAVRAAHLRFCNSAEGL
                     STRPALEQLAAFQRLD"
BASE COUNT          316 a          503 c          492 g          297 t
ORIGIN      
        1 cggaaacgcc cagcggcaat tcctcgacct gcttgggtcg taccgcgtgg cgtgtctcga
       61 gccagattcg cgccagcacc tggctggttc gcgaaccggc atcgagcgcc agccgctgga
      121 cgcggtgcca cggcaccctg ctgaacagct tcacactgcg gactggcccg tgagccgcga
      181 tcgcaaagcc cgggatgatc tcataacccc ggacagcacc acgcaaatac tcgaccgacg
      241 ggattcagag caacgtccag ctcgccgtca gagagctgct cggcaagccg gctagggact
      301 tccatcgata ggcgaatctg gggaaagtgt tcgcaaagcc cataatagag gggctttgcg
      361 ttcaagtaac ttacggctcc gacacggatt ggcggggcca tgaccttcag ccctcctagt
      421 tgacaaccga ttcccgacgc gtatcgccgg agccactcac tagatgattc gtacgtcctg
      481 acgggacttt cagaatcttt tgaaatttta gtcgcgaacg ggcgaaccgc aaggccactc
      541 ttttgatatc aaggggggga cgttttccaa cgatttccct gtacctagcc gttgcggcct
      601 tccccatctt aacgccgggt tggtattcac atggcgcttt tggtctcccg gaacctctcg
      661 aacctggtgt cccgggatcc agacctgatc agggattgac cgccggccgg tggcggcgcg
      721 cagggtccca cagccggaaa ctcacgtcgc gatccgtagc cagcaacgaa gtcaactcgg
      781 cgtcgccgcc atcgtcgcgg ccatcctcgg ccacggagta gatgacgatt cggtctgcga
      841 agcgccgcaa gtgcaggggc tcgccgccaa acgggtccgt cgcaaccttc gcaaggaaat
      901 cggggatgag ggattcaagc gccgacggcc agatgcttcg ggcgagtcgg tagcgctcgg
      961 cagccaccgc gaccgacgca caccgcagtc gagcccgact gttctggaag gcgctggcca
     1021 tctggcgaat tgccgggacg atggcctgca cgtctgtcgg aaggtgcttg acgcttcaat
     1081 caagccgctg gaaggcggcc agttgctcaa gcgccggccg tgttgataac ccctcggcac
     1141 tattgcaaaa ccgcagatgc gccgcgcgga ctgccgttga gttgcgaaag acgtgcaaga
     1201 tcgtggagaa aaaggtcgtg tttcggacct gcctaggcgt gaactccccc gacgcgacag
     1261 acgtgaggag ccggtccatg agcgcccgct cgcctttgat ggccgatacc agcaacggct
     1321 cgtcttcctc cgcgtcgagc gcgtgttgga gtctcgcgag cacggcctct gaagctcggc
     1381 cgtgtgccag ggcgaattcc acctgctgac aggtcgtcat gcgtatatcg atccgcaacc
     1441 gctgtgacac ggccgtgggc tcgtcgccga ttgaccggcc cacattcaac agggcatggc
     1501 aggtcaacaa ggcctcgtct gtttcctgct tctgggaccg cagtaagaca tcataggcca
     1561 gcaggttgac gacatcgcgc gccgtcgccg gatggcgaag ttgggtag
//
LOCUS       DATAQL010000624         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2965212, whole genome shotgun sequence.
ACCESSION   DATAQL010000624 DATAQL010000000
VERSION     DATAQL010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965212"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHS97_10885"
     CDS             <1..355
                     /locus_tag="VHS97_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX3448750.1"
                     /translation="HLGAGYFEAVYGNALEIEMNARNIPFERHKEVEVFFKGESVGKG
                     EVDFFVGGILVVELKTVETFAPIHLAQVISYLKALNRPLGLLINFKVPMLRDGVKRVA
                     FGADLTNDSEALASS"
     gene            565..1947
                     /locus_tag="VHS97_10890"
     CDS             565..1947
                     /locus_tag="VHS97_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448751.1"
                     /translation="MGVTLGWVALALAALGITPQSEREKPPITYTVRMVEADGVGWRE
                     AVFDCLKPVTRQGSATVWTLPLDASTRLLTEVTKSPAARVVQVPKVTSQSGSPATIQC
                     RSNRQLVIQTTWNNDEASPASSPENIRVGWHTTMVGRKLDQGILVQMVFEDTLVRAVH
                     HVSVHRSVASNGARASAAAAGCEPQVFAGGGKATGLMGIAQTALKGAGFDAICSESQT
                     SCQAAGSRTKDEALATASFDVPEIDNREVLGEWLIPRGDALLVSFGAFTVADNDGKAI
                     VKERLALIEAVDAQSAVPGALVPKVALPALSTTIPPPAPLVPHLLPTVPELPKMALPS
                     PVMPSRSIPQGVHADGTPADLPPLPDDETKDAPPESESADPMPMPSPQIKKPSAPKAK
                     PTTDANTSKAEFALPKAATMFLPSLFLPSPSVGFQFLLPIKPLSFKLPFGQRIEIEVF
                     GRIVPDSETR"
     gene            complement(1963..>2151)
                     /locus_tag="VHS97_10895"
     CDS             complement(1963..>2151)
                     /locus_tag="VHS97_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3448752.1"
                     /translation="ELTEKAREEIPGLHERVLSRIPQGRWAKPADMAGTAVWLASSAS
                     DYVTGVAIPVDGGYASSL"
BASE COUNT          419 a          687 c          633 g          412 t
ORIGIN      
        1 ccacctgggt gccggttact tcgaggcggt ctacggaaat gccctcgaaa tcgagatgaa
       61 cgcccgcaac attccctttg agcgccacaa ggaggtagaa gttttcttca agggcgagtc
      121 cgttggcaaa ggtgaagtcg atttcttcgt tggcggtatt ctggtggtag aactcaagac
      181 cgtggaaacc ttcgcaccga tccatctggc gcaggtcatt tcctacctga aggccctgaa
      241 cagaccgctt ggcctcttga tcaacttcaa ggttcccatg ctcagggacg gcgtgaagcg
      301 tgtcgctttt ggggccgatc tgaccaacga ctccgaggcg ctcgccagtt cctgacctgt
      361 cagattgccc gccggtatgc accaattcaa ttttgtcctg gcggttccga atcaggctca
      421 agttttcttg gcggtttgcc gacagccgcg attgacgcga tcgaggatcg accgtatgat
      481 cccgctcgaa tcgctctggt ctggcgacca gcatggatgc cgaacgaact cgacgggcaa
      541 ggtggacgga agggaggctg tcacatgggg gtcacattag gatgggtcgc gctcgcgctc
      601 gccgcgctgg gaatcactcc gcaatcggaa cgcgaaaagc caccgattac ctacacggtc
      661 cgaatggtcg aagcggacgg cgtgggttgg cgcgaggctg tgtttgactg tctcaagccg
      721 gtcacgcgcc agggatcggc caccgtatgg actctgcccc tcgatgcgtc gactcgcctc
      781 ctcacagaag tcaccaagag cccggcggcc cgtgtcgttc aagttcccaa agttacctca
      841 cagagcgggt cgcccgcgac gatccaatgt cggagcaatc gtcagctcgt catccaaacc
      901 acctggaaca acgacgaagc gtcgccggca agtagtcccg agaacatccg cgtcggctgg
      961 cataccacga tggtcggtcg caagctcgat cagggaatct tggtccagat ggtgtttgag
     1021 gatactctgg ttcgggctgt ccaccacgtg agcgtgcatc ggtccgtcgc gtcgaacggc
     1081 gcgcgggcat ccgccgccgc tgcggggtgc gagccgcaag tcttcgccgg tggcggcaag
     1141 gcaacggggc tcatgggaat cgcccaaact gctctcaaag gcgccggctt tgatgccata
     1201 tgctccgagt ctcagacgag ctgccaggcg gccggcagtc ggacaaaaga cgaggcgctg
     1261 gccactgcct cctttgacgt ccccgagatc gacaaccgcg aggtgctggg cgagtggctg
     1321 atcccgcgcg gggacgcttt gctcgtcagt ttcggtgcct ttaccgtggc cgacaacgac
     1381 ggtaaggcga tcgtcaagga acgcctggcg ctgatcgagg ctgttgacgc acaaagtgcc
     1441 gttcccggcg cgctcgtccc gaaggtggca cttccggcgc tctcaacgac aattccgccc
     1501 ccggctcctt tggtacccca cctgcttccc acggtcccgg aactgcccaa gatggcactg
     1561 ccgagtcctg tcatgccaag ccggtcgatc ccgcagggcg ttcacgccga cggcacgccg
     1621 gccgacttgc caccgctgcc cgacgacgag accaaagacg caccacccga atccgagtcg
     1681 gccgatccga tgccgatgcc cagccctcaa atcaaaaagc caagcgcgcc caaagctaag
     1741 ccgacgaccg acgccaacac gagcaaggcc gaatttgcat tgccaaaagc ggcgacaatg
     1801 tttctgccga gcttgttcct tcccagtcct tccgtcggct ttcagttcct gctgccaatc
     1861 aagccactgt cgttcaagct cccgtttggc cagcgcatcg agatcgaggt ctttggcagg
     1921 atcgttcccg acagcgaaac acgctgattc cattccattc catcacaacg acgacgcgta
     1981 gcccccgtcc acggggatgg cgacgcctgt gacatagtcg ctggccgaac tggccagcca
     2041 gacggccgtg cccgccatat cggccggttt ggcccatcgc ccttgcggaa ttcgagacag
     2101 gacgcgctcg tgaaggcctg ggatctcttc tcgggccttc tcggtaagtt c
//
LOCUS       DATAQL010000625         3612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_64532, whole genome shotgun sequence.
ACCESSION   DATAQL010000625 DATAQL010000000
VERSION     DATAQL010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64532"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHS97_10900"
     CDS             complement(<1..224)
                     /locus_tag="VHS97_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3448753.1"
                     /translation="MGLPRVSALFDWADNTPWDAVVIGAGPAGAMAARELALLGGRIL
                     LVERKRFPRWKICGACLNGHALACLRAAGLG"
     gene            complement(224..943)
                     /locus_tag="VHS97_10905"
     CDS             complement(224..943)
                     /locus_tag="VHS97_10905"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3448754.1"
                     /translation="MRPQFLTSLSRRRRQAELMDQEGLGETEHFAALQGLARINWLSR
                     SDAILWPHIERLARANRGATIRVLDLASGGGDVPIALARRALRAHLNVRIEGCDVSQQ
                     AIAFASRKGAAAGLAVSFFTLDVLNQALPAGYDVVTCSLFLHHLDDADAGPFLRKAAD
                     ATGRLLLINDLVRGPVAFALAWSGCHLLSRSPVVRYDGPVSVAAAFSLAEARMLAQRA
                     GLCGANLVRRWPCRFLLSWSR"
     gene            complement(1068..2444)
                     /gene="hslU"
                     /locus_tag="VHS97_10910"
     CDS             complement(1068..2444)
                     /gene="hslU"
                     /locus_tag="VHS97_10910"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX3448755.1"
                     /translation="MPKHERERELTPRQTVAELDRDIVGQAEAKRAVAIALRNRWRRR
                     QLSDDLRQQVTPKNIMLIGPTGVGKTEIARRLAILVGAPFVKAEATKYTEVGYVGRDV
                     EGLIRDLVEASIVLVRNAERLKVVDPAKERVEQRLLDLLLPAAPAASWQDGDTPQAAE
                     AAERRQRSREKMKARLQSGELEDRDVEVTFPGRSMAPVSILGAGNFEQMEMDLQSMFE
                     KIMPKQSQTRRLTVGQARPILLQQEIDGLIDPEKITQAAVNLTEESGIVFIDEIDKIT
                     SDEGSNRGPDVSRQGVQRDLLPIVEGTTVNTKHGPVKTDHILFIAAGAFHRSKPADLM
                     PELQGRFPIRVEMHDLTRDDFARILREPRASLLRQYQALLGTEGITLEFADAAIETMA
                     DLAYHVNRSTQNIGARRLHTILERVLEDLSFDASDRTDKHVVIDEAHVRERLDALSQD
                     EDLSRFIL"
     gene            complement(2414..2974)
                     /gene="hslV"
                     /locus_tag="VHS97_10915"
     CDS             complement(2414..2974)
                     /gene="hslV"
                     /locus_tag="VHS97_10915"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX3448756.1"
                     /translation="MDWHATTILSVRKGDRVAIGGDGQVTLGTQVMKADAVKIRKLLD
                     GQVLVGFAGSAADGFALLERFEGKLKDYPNNVPRAATELAKLWRTDRMLRRLEAVLVV
                     VDSRHSLLLTGSGDVIQPSDGILATGSGGGYALAAARALIKHSALSAAEVVRESLSIA
                     GGIDIYTNQHLTVEELPCQSTSANVS"
     gene            3238..3444
                     /locus_tag="VHS97_10920"
     CDS             3238..3444
                     /locus_tag="VHS97_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX3448757.1"
                     /translation="MCLRVILDREDDGRWFAEIPELPGVLVYGQTEDDAITKVKALCL
                     RVLADRLEHGEVVPDVDELFSVTP"
BASE COUNT          663 a         1145 c         1099 g          705 t
ORIGIN      
        1 cccaatccgg cggcgcgcag gcatgcgagc gcatggccgt tcaagcacgc gccgcagatc
       61 ttccaccgtg ggaatcgttt tctctcaacg agcaagatgc gcccgcccaa gagcgccagt
      121 tccctcgcgg ccatcgcccc agccggtccc gcgccgatga cgacagcgtc ccatggggtg
      181 ttgtcggccc aatcaaagag cgcagaaacg cggggcagcc ccatcaacga ctccacgaca
      241 aaagaaatcg acacggccag cgccgaacca ggttcgcgcc acagaggccc gcgcgctggg
      301 cgagcattcg ggcttcggca aggctgaacg cggcggcgac cgagaccggc ccgtcatacc
      361 ggacgaccgg tgaccgcgac aacaggtggc agcccgacca cgccagcgcg aaagcaaccg
      421 gtccccgaac gagatcgttg atcaagagga gtcgaccggt cgcgtcggcg gccttccgca
      481 agaaagggcc ggcgtcggcg tcgtcgagat gatgcaagaa taacgaacac gtcacgacat
      541 cgtaacccgc cggcaacgct tggttgagaa cgtcgagtgt gaaaaacgag acggctagcc
      601 cggcggcggc ccctttccga cttgcaaatg caattgcttg ttggctgaca tcacaacctt
      661 cgattcgcac gttcagatgc gctcgcaagg cccgtcgggc cagcgcgatc ggcacatctc
      721 ctcctccact ggcgaggtcg agaacgcgga ttgttgcgcc ccgattcgcc cgtgccaacc
      781 tctcaatgtg tggccaaaga atcgcgtcgc ttcgactcag ccagttgatt cgagccaggc
      841 cctgcaaagc ggcgaaatgt tcggtctcac caagcccctc ttgatccatc agctccgcct
      901 gccgtcgccg ccgcgataat gacgtcagaa actgcggtct catggcaccg actttccact
      961 agaagacccg ttgttgccgg gttttgactt gcccctggcg cggttaacgg ctgttgcgga
     1021 ccgctggcgc tagtggcctt gatccggtgg gcggcgcgcg ccctgcacta caagataaac
     1081 ctgctcaggt cttcgtcttg cgagagtgcg tcgagtcgct cgcgcacgtg ggcttcatcg
     1141 ataacgacgt gtttgtcggt acggtcggag gcatcgaagc tcaagtcttc gagcacccgt
     1201 tccagaatcg tgtgcaggcg gcgggcgccg atgttctggg tcgagcggtt cacgtggtat
     1261 gccaggtcgg ccatggtttc gattgccgcg tcggcgaatt cgagcgtgat cccctcggtc
     1321 cctaacagtg cctggtattg ccgcaacagc gaggcgcgcg gctcgcggag gatgcgggcg
     1381 aagtcgtcgc gcgtcaggtc gtgcatctcg acgcggatcg gaaagcggcc ttgcagctcg
     1441 ggcatcaggt ccgccggctt cgatcgatga aacgcgccag cggcgatgaa gagaatgtgg
     1501 tcggtcttga ctggtccgtg cttggtgttg accgtcgtcc cctcgacgat cggcaacagg
     1561 tcgcgctgca cgccctgacg cgagacgtcc gggccgcgat tggaaccctc gtcgctggtg
     1621 atcttgtcga tctcgtcgat gaagacaatt cctgattcct cggtcaagtt cacagcagcc
     1681 tgggtgatct tttcgggatc gatcaacccg tcgatctcct gctgcagcaa gatcggccgc
     1741 gcctggccaa cggtcagccg tcgcgtctgc gactgcttgg gcatgatctt ctcgaacatg
     1801 ctctggaggt ccatttccat ctgctcgaag ttgcccgctc ccaggatcga cacaggcgcc
     1861 atcgaacggc cgggaaaggt gacctcgacg tcgcggtcct cgagctcacc ggactgcaat
     1921 cgcgccttca tcttttcgcg cgaccgctgg cggcgttcgg cggcctcggc ggcctggggc
     1981 gtgtcgccgt cttgccacga cgcggcagga gcagccggca agagcaggtc aaggagtcgc
     2041 tgctcgacgc gttccttggc cgggtcgacg accttcaacc gctcggcatt gcgtaccagg
     2101 acgatcgagg cctcgacgag gtcgcgaatc aggccctcga cgtcgcgacc cacatagccg
     2161 acctcggtgt acttggtcgc ctctgccttc acgaagggcg cgcccacgag tatcgccaac
     2221 cggcgggcga tctcagtttt gccaacgcct gtggggccga tcagcatgat gttcttcggg
     2281 gtcacctgct ggcgcagatc gtccgagagc tgccgccggc gccaccggtt tcgcagcgcg
     2341 atcgcaacgg cccgcttggc ttcggcctgg cccacgatat cgcggtccag ctcggccacg
     2401 gtctgccgtg gtgtcaactc acgttcgcgc tcgtgctttg gcacgggagt tcctccaccg
     2461 tcaagtgctg attggtatag atatcgatgc caccggcgat ggacagcgac tcgcggacga
     2521 cttcggccgc cgacagcgca gagtgcttga tcagagctcg cgcggctgcc agtgcatagc
     2581 cgcctcccga tccggttgcc agtattccgt cactgggctg gatcacgtcg cccgacccgg
     2641 tcagtaaaag gctgtgccgc gaatcgacga cgaccagtac agcctcgagc ctccgcagca
     2701 tgcgatcggt acgccacagt ttggcgagct cggtcgcggc ccgtggcacg ttgttggggt
     2761 aatccttgag ctttccctcg aatcgctcca agagcgcaaa cccgtcggct gccgagccgg
     2821 caaacccgac cagcacctgg ccgtcgagca gcttgcggat cttgaccgcg tcggccttca
     2881 tgacctgggt gcccagcgtt acctggccgt cccccccgat cgccacccgg tctcccttgc
     2941 gcaccgaaag gatcgtcgtc gcatgccaat ccaattccca ctcccttcgc ggttgtgcct
     3001 cgaggttttg cggtcgcttt ggcccattac attgtaactg acaccgcaag ggttgctcat
     3061 cgctcgtttc gtctggaatc ttccttgcaa gcataattgc attgacggta attcaccagg
     3121 tgggcggaat cggggaacgg cggacagaca gcgtcattga ggttagaata gtatcagtct
     3181 cgaatgagca gatgcttgat ctttcgaccg aaaagtgcaa tcaccggagc gtaacgaatg
     3241 tgcctcaggg tcatcctgga tcgcgaagac gacgggcggt ggtttgcaga aatccccgag
     3301 ctccctggcg tcctcgtgta tgggcaaacc gaggatgatg cgatcaccaa ggtgaaagca
     3361 ttatgtctcc gcgttttggc cgatcgtctc gaacatggcg aggtcgtccc cgacgtggat
     3421 gaactcttct cggtgacccc atgagtgagt ggccgtcaac ctaggcccgt aatgtcctgt
     3481 cagcgttact ttcaacttga tcaagaccgt gaagaagcct tttgttcttg gggcaattgc
     3541 gcgaaaagct cgcacaaatg cggctcgtag tgtttccagc gagcgaccga tcgctgataa
     3601 accggttggc gg
//
LOCUS       DATAQL010000626         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1744912, whole genome shotgun sequence.
ACCESSION   DATAQL010000626 DATAQL010000000
VERSION     DATAQL010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744912"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VHS97_10925"
     CDS             complement(<1..782)
                     /locus_tag="VHS97_10925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3448758.1"
                     /translation="MPDLDAEMLIDQAVMIGLVSREQLREARAEAEDGSPDTDLRMLL
                     RKGSLTSWQVERLKKGDPSSFFYGDAKALFHLAEGTFARVYRGEHTTSKAPLAIKVLR
                     QRFVNISEAVERFHKEAEAGMRLRHPNIVQIIDVGQREQRHFMIMEYVEGMNLRDFMK
                     LRTRIPTEQAVPLMLGMAHGLQYSLERGVTHRDIKGTNILISNSGVAKLVDFGLATML
                     RDESKPGVKSQRTVDYSALERTCNSPKGDPRSDIYFLGCVYY"
     gene            1386..1745
                     /locus_tag="VHS97_10930"
     CDS             1386..1745
                     /locus_tag="VHS97_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448759.1"
                     /translation="MSKTSSRDVASDHMNAADDPSSAWTNRRATEQATRAATARQNSG
                     RRRFVDPTTSEREYSKAEMEFMMAMNAYKQSSGRMFPTWSEVLEVLRGLGYEKVSQEQ
                     LHEVALERRSATRLAAR"
     gene            1937..>3411
                     /gene="gltX"
                     /locus_tag="VHS97_10935"
     CDS             1937..>3411
                     /gene="gltX"
                     /locus_tag="VHS97_10935"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX3448760.1"
                     /translation="MSVRTRFAPSPTGYLHIGGVRTALFNWLLARHHGGQFILRIDDT
                     DQQRHVEEAVGRILDGFRWIGIDWDEGPEVGGPLGPYYQSQRTESYVRAADQLVASGH
                     VYRDYSTDSERAADKAAAERQKRAYRFRRNPLSEDEAARFQAEGRPFALRFQVPPGRT
                     LVLNDLIKGPVEFSTDEIGDFVIVRPDGTPLYNFASVVDDAAMRITHVVRADEHLSNT
                     FPQLLMFEALGATLPAFAHIPYVAEPGSKLKMSKRKTEEYEKSGILVYLHQYIDRGYL
                     PDALLNYLARLGWSFDGTQEIFTRPELIEKFSLERVNSSAASHDQDKLFWIEGEWMKK
                     LPLEDKVEGVLPFLKKEALVSDPMSPSERTRIAAVITALGDRLKVFADILKLGRFFFT
                     ESLAYEGDAVKKRLRKVGVAAMLDELDSVLAQVEPFDLASLESAVHAYAESTGRKMGD
                     VVNPLRVAVTGQGIGPGLYDCLAILGRDLCRARVRQTVEML"
BASE COUNT          697 a         1023 c         1005 g          686 t
ORIGIN      
        1 tgataataga cgcatccgag gaagtagata tcggaacggg ggtcaccctt ggggctgttg
       61 cacgtgcgct cgagggccga gtaatcgacg gtgcgctggc tcttgacgcc cggcttggat
      121 tcgtcccgca gcatggtggc caaaccaaag tcgaccagct tggcgacccc cgagttggaa
      181 atcaggatat tggtcccctt gatatcgcgg tgagtcacac cccgttccag agagtattgc
      241 agcccgtgcg ccatgcccag catgagcggc acggcctgct ccgtggggat ccgcgtacgc
      301 agcttcataa agtcgcgcag gttcattccc tcgacatatt ccatgatcat gaaatgtcgc
      361 tgctcccgct gacccacgtc aatgatctgg acgatgttgg gatgccgcag ccgcatgccg
      421 gcctcggcct ctttgtgaaa ccgctcgaca gcttcgctga tgttgacaaa ccgctggcgg
      481 agaaccttga tcgccagggg tgccttgctc gtggtgtgct cgccgcgata gacccgagcg
      541 aaggtgccct ccgccaggtg gaagagcgcc ttggcgtcgc catagaaaaa gctgctgggg
      601 tctcccttct tgagccgctc gacctgccag ctcgtgaggc tccccttacg tagcagcatc
      661 cgtaggtccg tgtcgggtga tccgtcctca gcctcggccc gggcctcgcg aagctgctcg
      721 cgcgatacca agccgatcat cacggcttgg tcgatcaaca tttcggcgtc aagatcgggc
      781 atggacagtc gatcctcgcc ttggctggaa gtgaaatggc tggaaatgaa acaggaaaac
      841 gtaatgatcc tgattctagc aagtcacgcc cgtcggtttc tatgctcccg atcgaggatc
      901 ttggcgatcg cgtctgtgat tggccaaatc agtcggccgc tcaactccgg gcggccaacg
      961 gggaatatcc aagtatgcgg ggtatccgac gccatctaga accatcacac accttggacc
     1021 agcgcgcaca gcgattcccc cgacactatg actacgatct cgatcctccc gatgtgaccg
     1081 gcgtttgtgg atcagtgccg gcacccacaa gaggccggct cgtccacgct gaatctgacg
     1141 cgcctcccgc cacgaaacgg gtcgaataat ccaaaaaaat attgagtgtg acactcatga
     1201 tgtcaccgcg atggattagg ttcatctgca ggagtggtcc ccgcgtgaag tcacacccct
     1261 ctcacgagac ggctgcaaac cgccgcgcgg agaatcgtca cgccaggttt cttcccaggt
     1321 gccgcctgac tcacagaacg gtttttatca cgatcttggc aggcgatcag aggagctaga
     1381 agcggatgag caaaacgtcc tctagggatg tcgcaagcga tcatatgaat gcggccgatg
     1441 acccgtcgag cgcatggacc aatcgccgtg ccaccgagca ggcgactcgg gcggctaccg
     1501 ccaggcaaaa tagcggccga cgccgtttcg tcgaccctac aactagtgaa cgagaatata
     1561 gtaaggcgga aatggaattc atgatggcca tgaacgcgta caagcaaagt agtggtcgga
     1621 tgtttcctac gtggagcgag gttctcgaag tgcttcgggg tctcgggtat gaaaaagtga
     1681 gccaagagca actgcacgag gtggcgctcg agcggcggtc tgccacgcgt ctcgccgcgc
     1741 ggtgagggcc aggccgttcg tgcctcacga tcgcccggag agtctagcga gatggaaacg
     1801 ctccgggttg atctgggtct accgagtgca tgctgggccc gttgacagcc tggttcaaac
     1861 accctatcgt gagcttgatt cgttcttacc ctagtttagc gccgtcgcgt cgggttgtag
     1921 ctttcgtttg gatctcatga gcgtacgcac tcgtttcgcc ccgagcccca ccggctatct
     1981 gcacatcggc ggggttcgga ccgcattgtt caactggctc ttggcgcggc accacggcgg
     2041 ccagttcatt cttcgaatcg atgatacaga tcagcaacgg cacgtggagg aagccgtcgg
     2101 gcgcatcctc gatggcttcc gctggatcgg tatcgactgg gatgaagggc ccgaggtcgg
     2161 cggtccgctc ggcccgtatt atcaatctca acggaccgaa agctatgtgc gggccgccga
     2221 ccaattggtc gcctcgggac acgtctatcg cgattacagt accgacagcg agcgcgccgc
     2281 cgacaaggcc gccgccgaga gacagaaacg cgcttatcgg ttccgccgca accccctctc
     2341 cgaagacgaa gcggctcgct ttcaggccga gggacgtccc ttcgccctcc gctttcaagt
     2401 cccaccggga cgcacgctcg tcctgaacga cctcatcaag ggccccgttg aattctcaac
     2461 cgacgagatc ggcgatttcg tgatcgttcg gccggacggc actccgctct acaatttcgc
     2521 cagcgtggtc gatgacgccg ccatgcggat cactcatgtt gtgcgggccg atgaacacct
     2581 ctcgaataca ttcccgcagc tcttgatgtt cgaggcccta ggcgcaacct tgccagcctt
     2641 tgctcatatt ccctacgttg ccgaaccggg ctcgaagttg aaaatgtcca agcgcaaaac
     2701 cgaggagtat gagaagagcg gaatcttggt ctatttgcac cagtacatcg accggggcta
     2761 tctccccgat gcgctcctca actacctggc ccgcctgggc tggagttttg acggcacgca
     2821 agagatcttc acgcgtcccg agttgatcga aaagttctcg ctcgagcggg tcaacagctc
     2881 cgccgccagt cacgatcagg acaagctttt ctggattgaa ggggagtgga tgaaaaagct
     2941 ccccctggaa gataaagtgg aaggtgtgct gccgttcctg aaaaaagagg ccttggtcag
     3001 cgatccgatg tcgccatccg aacgcacgcg gatcgcggcc gtgatcacgg cgctgggcga
     3061 tcggctcaag gtctttgctg atatcctcaa gctgggccgg ttcttcttca cagaatcgct
     3121 ggcgtacgaa ggcgatgccg tcaaaaaacg gctacgaaaa gtcggtgtcg cggcgatgct
     3181 ggacgagctc gactcggttt tggcccaggt cgagcccttc gatctggcgt ccctcgaatc
     3241 ggccgtacac gcctacgccg aaagcacggg ccgcaagatg ggagacgtcg tgaacccatt
     3301 gcgcgtggcc gtcacgggtc aaggcatcgg tccaggcctt tacgactgcc tggcgatcct
     3361 ggggcgcgac ctttgccggg cccgcgttcg ccagactgtc gaaatgttgc a
//
LOCUS       DATAQL010000627         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1590198, whole genome shotgun sequence.
ACCESSION   DATAQL010000627 DATAQL010000000
VERSION     DATAQL010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590198"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3039
                     /locus_tag="VHS97_10940"
     CDS             <1..3039
                     /locus_tag="VHS97_10940"
                     /inference="COORDINATES: protein motif:HMM:NF042565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1583 domain-containing protein"
                     /protein_id="HEX3448761.1"
                     /translation="LDACIEGFESAPSQQGEPLGTLLIMMARRQFQLGDAPGGRGRLY
                     AYFEVIEKNTTRYSGDYSFYLRKEQIQRVAGEFARAGMWSDALAALARHFDRPTPIGG
                     YPPVDDVLERVLRGLAALPARERYEALRTWTMAAKDRRVVRILTSLATRDTAPEFFAR
                     AAINQMPTVGPAGAGSDDTRPVLLSTATALIDAARQAGTLDQLAEEVSAAAAARVGTQ
                     EIENAEVLHLLVELARGQGTKVAAGIEGRLAALIKENEAHPVDAARPYIYVQIGITGS
                     DRVNFPWTDYLLARSTFGANEPIGGLGLRLMQALVDRAEWFKDWTLISIIRGGLAEAT
                     AHRAGAPDVLAEAGLAFWHPTNMRLSLYLSACEPASHWVAHQGHVRQTSGSTTDLLLF
                     DYPLTGSYEVSVDTYSGPRGHAVAVHNGLGIAPFGPEENGATARVLVAGHTERIPIHW
                     RLSRPHGFNRLTVAATPENVRYLVNGHLFYEEDNPSPTSPWIGLLSGGQRQSTWRNLS
                     LRGTPIIPREVRLSQGDRLQGWVPNFYRETVPLQLGTASYHANAPPTGISGIGFKRRM
                     PGPALKSQTTTRKETINPDDFDWAAQDGVIHGRRVLRENRLQTSVASDSDVKGAEADQ
                     SRLYYQRPLRDGDSIDYEFLYEPGQVMVHPSIDRVAFLLEPDGVKVHWITAGGDDRSG
                     LTADNAIEEPNNRRGPNALPLKPGEWNGIKVALDGDKVTLTLNAQVIYERIMEASLGR
                     QFGLFHYKDQTAVAARNVVLRGRWPALLTPQQMANLMAPDPAAPHSDADRRARHALIG
                     EPIFALQAGEVVEKSRKLAPEERYHQLLEWVLPSADHPVFRLEGDSTPSLPATSDSLP
                     GAPDENGSGRGVRVQADGEILAPALELVDTARLLGKLDELATLVAQAQVPDDDNRTIN
                     ERGKRSLLALVHISRGDDVAAHKVVEELMPMLATIPLDQPEWVRWPELALASRAIERP
                     SLRRHTRAILEALVAQAKKQSPNALWAQRVTNLLARAELL"
BASE COUNT          625 a         1072 c         1007 g          593 t
ORIGIN      
        1 cttgatgcgt gcatcgaggg gtttgagagc gccccgtccc agcagggcga gccgttgggc
       61 acgctgctga tcatgatggc tcgtcgccag ttccaactcg gcgacgcgcc cgggggccgc
      121 ggacggcttt atgcctattt cgaggtcata gagaagaata cgacccgtta ttccggcgac
      181 tattcctttt atctccgcaa ggagcagatc cagcgtgtcg ccggtgagtt cgcccgcgcc
      241 ggcatgtggt ccgacgcgct cgcggccctg gcccggcact tcgaccggcc aacccccatc
      301 ggcggctatc cgcccgtgga tgacgttctg gagcgagttc tgcgcgggct ggccgcactc
      361 ccggccaggg agcgatacga ggcgttgcgg acgtggacca tggccgccaa ggaccgacgt
      421 gttgtgcgga tcctgacttc gctggcgact cgcgacacgg cgcccgagtt tttcgcacgc
      481 gcagcgatca atcaaatgcc cacggtgggc cctgccggcg ccggaagtga cgacactcgg
      541 ccggtacttc tgagtacggc aactgcctta atcgatgcgg cgcgccaggc cgggacgctc
      601 gaccagcttg ccgaggaagt cagcgccgcc gccgccgcac gggtgggtac gcaagagatc
      661 gagaatgccg aggtcctgca ccttctcgtc gagctggcac gcggccaggg gaccaaggtt
      721 gcagccggga ttgagggccg cctcgccgca ttgatcaagg agaacgaagc gcatcccgtc
      781 gacgcagcca ggccatacat ttatgtccaa atcggcatta ccggttccga ccgggtcaat
      841 ttcccgtgga ccgattacct gctggcacgc tcgacattcg gtgcgaacga gccgatcggc
      901 ggcctcggcc tgcggttaat gcaagccctg gtcgatcgtg cagaatggtt caaagactgg
      961 accttgatat cgatcatacg tggcggtctg gccgaggcca cggctcaccg ggcgggcgct
     1021 ccggacgtgc ttgccgaagc cggcctggca ttttggcacc cgaccaacat gcggctgagc
     1081 ctttatctct cggcctgcga gcctgcctcc cattgggtcg cccaccaggg gcatgtccgc
     1141 cagacgtccg ggtccacaac ggatctcctg ctcttcgact atcccctcac cggttcgtac
     1201 gaggtttcgg tagacaccta cagcggcccc cggggccatg cagtcgctgt ccacaatggc
     1261 ctcggcatcg ctccgttcgg gccggaagag aatggggcaa ccgctcgggt cctcgtcgcc
     1321 ggtcataccg agcgtatccc catccactgg cggctctccc gccctcacgg cttcaaccgc
     1381 ctgaccgttg cggccacgcc cgagaatgtt cgctacctgg tcaacggcca cctcttttac
     1441 gaggaagaca acccaagccc gactagcccc tggatcggct tacttagcgg cggtcagcgc
     1501 caatcgacct ggagaaatct ctcgctacgg ggaacgccga tcatcccacg cgaagttcgg
     1561 ctcagccagg gtgatcggct ccaaggctgg gtgcccaact tttaccgtga gaccgtgccg
     1621 ctgcagctgg gcacagcgtc gtaccacgca aacgcgcccc caacgggcat aagcgggatc
     1681 ggtttcaagc gccgaatgcc cggaccagca ctgaagtcac agactacgac gcgcaaggaa
     1741 acgatcaacc ctgacgactt cgactgggcg gcccaagacg gtgtaattca cggccgtcgt
     1801 gttctgcgtg aaaacaggct gcaaacttcg gtcgcgtccg attccgacgt caagggtgcg
     1861 gaggccgacc aaagccggct ctattatcaa cgcccgctcc gcgatggcga ctcgatcgac
     1921 tacgaattcc tgtacgagcc cggccaggtg atggtccacc cgtcgatcga tcgggtggcc
     1981 ttcctgctcg agcccgacgg tgtcaaggtc cactggatca ccgccggcgg cgacgatcgt
     2041 tccggcctca ccgccgataa cgcgatcgag gagccgaaca accggcgggg gcctaacgcg
     2101 cttccgctca agccgggcga gtggaacggg atcaaggtgg ctttggacgg cgataaagtc
     2161 actctcacac tgaatgccca ggtgatttac gaacgcatca tggaggcgag cctggggcgc
     2221 caattcggcc tttttcatta caaggaccag actgccgtcg cagcgcgcaa tgtggtgctg
     2281 cgcggccgat ggcccgcgct cttgacgcca caacagatgg ccaatctcat ggcccccgat
     2341 ccggcggcgc ctcactctga cgccgaccgt cgcgcgcgcc atgccctgat tggcgagccg
     2401 atctttgccc tacaggctgg cgaggttgtc gagaaatcca ggaaactcgc gcccgaagag
     2461 cgctatcatc agctcttgga atgggtactc ccgtcggccg atcacccggt cttccgcctc
     2521 gaaggagaca gcacgccgtc gctcccggct acgagcgatt ctcttccagg cgctccggat
     2581 gaaaatggct ccggacgcgg cgtccgtgtg caggcggacg gtgagatcct cgcgccggca
     2641 ctcgaactcg tggacacggc acgattgctc ggcaagctcg acgaattggc gacgctcgtg
     2701 gcacaagccc aggttccgga cgacgataac cgcaccatca acgagcgagg taagcggtcg
     2761 ctgctggcgc tggtccatat cagtcgcggc gatgacgtgg cggcgcacaa ggtcgttgag
     2821 gagttgatgc cgatgctggc gacgatcccc ctcgaccaac ccgagtgggt gcgctggccc
     2881 gagctggcct tggcctcacg agccatcgag cggccgtccc tccggcggca tactcgagcc
     2941 atcctggagg ctctcgtcgc acaggccaaa aagcagtctc cgaacgcgtt atgggcgcag
     3001 cgagtgacga atctgctcgc tcgggctgag cttctctagg ccgccgaacg ggcttcgatg
     3061 aaatacggca cattcaccaa caacccaagc gttgacaccg caaactagcg ctgcttcgat
     3121 ttcttatccg aagcggatgt cagggctttt gtcaccaagt tccgccagca gccgctcgat
     3181 gagcattaaa taaacacaaa aggggacata attgatattg agcgtccgag gatggcattt
     3241 tacaatcgtg gtgtgccaag aactgccaga gcctctgttg gcggcttgtg gtatcac
//
LOCUS       DATAQL010000628         4861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2506398, whole genome shotgun sequence.
ACCESSION   DATAQL010000628 DATAQL010000000
VERSION     DATAQL010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506398"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VHS97_10945"
     CDS             complement(<1..1110)
                     /locus_tag="VHS97_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3448762.1"
                     /translation="METARIAQIIDEMGTLLELRGENPFRVRAYHTAAQALVNLAGDL
                     PEMIADGRLKEVAGIGDTMYSKIVQLATTGHLPSYDELRRSTPPGLVALLRIPGLGPK
                     KIKMLHETLKIESMADLRAAGETGLIAKLKGFGGKTEAKILEGIAFVEKAGERILQSD
                     ALRLVAPILAAVRNHPQVIRAEVCGSLRRRAETIGDLDVLFSSKNAPAVLDTFVKLPQ
                     VATVLAHGPTKASVRLADGVQCDLRGVEDAQYPFALHYFTGSKAHNIVMRKRALARGL
                     SLNEYALTGADGPIPCETEADLFEALGLAEIPPELREDHGEIDAALAGRLPSLVERAD
                     LTGTFHCHSDWSDGGATLAEMAAAAQARGMQYLGIA"
     gene            complement(1245..1886)
                     /locus_tag="VHS97_10950"
     CDS             complement(1245..1886)
                     /locus_tag="VHS97_10950"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX3448763.1"
                     /translation="MLKCSTSLWSADLANLATEIKRVEPYSERFHLDVADGHYVPTML
                     FFPDLVKSIRKHTARPLEVHLMTTDPCIWIAPFIEAGADGVIFCFDSLEEPGTAIKQV
                     KDLRKFVGVSLLVTEPVGLLRPYWHTIDQVTIVGTPMGTEGTSMAPTIFDKIREARAE
                     IDKYSAKVEIQVDGGIRRNTVLQIARAGADWIVPGSLMFGEDPAAIRVWLGSL"
     gene            complement(1909..2880)
                     /locus_tag="VHS97_10955"
     CDS             complement(1909..2880)
                     /locus_tag="VHS97_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3448764.1"
                     /translation="MHLGIFAKTFPRPTLEETLDAVAGHPLTHVQFNMTCAGLPTLPD
                     RIDEDLCVWIARLFQERGMAMAAISGTFNLCDPDTARLQDNLRRLDILAAACRWLDTR
                     IITICTGTLDPEDMWKWHPDNVRRTTWETLVDSVRAAIKVADRNEVTLAFEPEMHNVV
                     NSVSKARRLLDEIDSRWLKIVIDPANLIHPGERSRIAEILDEVFDWLGRDIILAHAKD
                     PRFDELNDDAVAAREFLNCGEANSSFSHVMWDMSRKGRRSDDAPELRESLMRRIFYFW
                     GFYRRYMSWLKKIEYTGAVVMHGLNEFEVQANTVFLQRVIQWSLKER"
     gene            complement(2962..4038)
                     /locus_tag="VHS97_10960"
     CDS             complement(2962..4038)
                     /locus_tag="VHS97_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448765.1"
                     /translation="MIAKDERCLRIGVLGCGPIAQFAHLDACRKAKNAELYAICDLAD
                     DLRERMQEVHRPKVAYADYAAMLADPRVEAIVIAIADQFHVPAALRAIAAGKHVLVEK
                     PLGTAVEPCRALRDQAAAAKLIVQIGNNRRFDPGVAFARDFITGEMGQRMALKSWYYD
                     SVYRYTMTDNVQPIPVTSTQARRPEGDPKADRRRYYLLTHGSHLVDTARFLGGPIVGL
                     RARALERFGAYCWFIAVDFADGSLGHLDLQIPIRGDFQEGFQVQGEHGSVTGRVHLPW
                     YHKASDVECFRARDRQFHRILGEDAFTYKLQIEGFAATILDGLPQAGASADDGLAAVQ
                     AMVAIARSVETGAYVRLADVSGDV"
     gene            complement(4035..>4861)
                     /locus_tag="VHS97_10965"
     CDS             complement(4035..>4861)
                     /locus_tag="VHS97_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448766.1"
                     /translation="HVAFPLKFIKEDEDLYPDLDYSPVVERTRRAIAAIERVRDQRGG
                     LGLVFSPHVSAGPNQIVDLVLAVVDAGATGIMFSETYAGGTVRMVRDATKHLANPPAI
                     YGHNAGIGIKTRGIWREVIDFLARLDGIDFRQTAPVRPGSPYIRPYGAEWRASEEALT
                     RPVAGINPTMITRAGGLDQGNLILNLQDLESKGIAQNVLFLAGSAINTIKDKGGKPDP
                     RIGLDAMLQAIEVHRSGELKDVPMEDHLAALAALARRKKLAALGEALRQRYPGKVA"
BASE COUNT          926 a         1573 c         1430 g          932 t
ORIGIN      
        1 ggcaataccc aggtactgca tccccctggc ctgcgcagcc gcggccattt cggccagcgt
       61 cgccccgccg tcgctccaat cggaatggca gtgaaacgta cccgtcagat cggctcgctc
      121 gaccaacgag ggaagtcgac cggccagggc ggcgtcgatc tcgccgtggt cctcgcgcaa
      181 ttccggcgga atctcggcca gccccagcgc ctcgaaaaga tcggcttcgg tttcgcaggg
      241 gatcggaccg tcggctccgg tcaacgcgta ctcgttgagg gaaagccccc gcgccagcgc
      301 gcgcttcctc atcacaatgt tatgggcctt tgagcccgtg aagtagtgga gtgcaaacgg
      361 gtactgcgcg tcctcaacgc cccgcagatc gcattggacc ccgtccgcca gccgaacgct
      421 cgccttggtc ggtccgtggg cgagcaccgt ggcaacctgg ggtagcttta cgaaagtgtc
      481 gagaaccgcg ggtgcgttct tggaactaaa caggacgtcc agatcgccga tcgtctcggc
      541 ccggcgccgc agactaccgc agacctcggc cctgatgacc tggggatggt tgcgcaccgc
      601 cgccaggatc ggggcgacga gccgcagggc atcgctttgc agaatccttt cgccagcttt
      661 ttcaacgaac gcgatcccct ccaagatctt ggcctcggtc ttgccgccaa agcccttgag
      721 cttggcaatc aggcccgtct cgccggccgc gcgaaggtct gccatgctct cgatcttgag
      781 ggtctcatgc agcattttga tcttctttgg gcccagaccc gggatgcgca agagtgcgac
      841 caggcccggc ggcgtgctcc gccgcagctc gtcgtaggaa ggcaagtgac ccgtcgtggc
      901 gagttgcacg atcttcgagt acatcgtgtc gccgattccg gcaacctctt tcaacctgcc
      961 gtcggcgatc atttccggca gatcgcccgc caggttaacc agggcctggg cggccgtgtg
     1021 gtaggcgcgc acccggaacg ggttttcgcc ccgcaattcg agcagcgtcc ccatctcgtc
     1081 gatgatctgt gcgatccgag cggtttccat cgcggtcacc tcagttccgt tttatcaagc
     1141 ggacgcggaa caattcccaa agtggatgtt gaggatcggt agctccggca gtgcggactc
     1201 tagttccctg atcaattgcc ccttgcctcc caggccaatc tcagctacag cgagcccagc
     1261 caaactcgta tcgccgccgg gtcttcaccg aacatcagcg agcccggaac gatccagtct
     1321 gcaccagccc gcgctatttg aaggacggtg ttgcgccgga tgccgccgtc gacctggatc
     1381 tcgaccttcg ccgagtactt gtcgatctcg gccctggcct cccggatctt gtcgaaaatc
     1441 gtcggggcca tcgaagttcc ctcggtcccc atcggcgtcc ccacaatggt aacctgatcg
     1501 atcgtgtgcc agtacggcct gagcagaccg accggctcgg tgaccaacag cgaaaccccg
     1561 acaaacttgc gcaggtcctt gacctgtttg atcgcagtcc ccggttcctc gagcgagtcg
     1621 aagcaaaaaa tcacaccgtc ggctccggct tcgataaagg gcgcaatcca gatgcatggg
     1681 tccgtggtca tcagatgaac ttcgagcggc cgggcagtgt gtttgcggat cgatttcacc
     1741 aggtcgggaa agaaaagcat ggtcggcacg taatggccgt cggccacatc gaggtgaaat
     1801 cgctcgctgt acggctcgac gcgcttaatc tcggtggcca gattggccag atcagccgac
     1861 cagagcgacg tggaacattt cagcatgggg tcacctcgac tgtcggaatc agcgctcctt
     1921 caaagaccac tgaattactc tttggagaaa gacagtattc gcttgcacct cgaattcatt
     1981 caacccgtgc atcacgactg ccccggtgta ttcaattttt tttagccagc tcatataccg
     2041 ccgataaaac ccccagaaat aaaagatcct ccgcatcaac gactctctta actcgggcgc
     2101 gtcatccgag cgccgtccct tcctcgacat gtcccacatc acatggctga aagacgagtt
     2161 ggcttcgccg cagtttaaga attcacgcgc cgcgacggca tcgtcattta gctcgtcgaa
     2221 gcgcggatcc ttggcgtggg ccagaatgat gtcccggccg agccaatcaa acacctcgtc
     2281 caggatttcc gcaatccgtg atcgttcccc cgggtgaatc aaattcgccg ggtcaatcac
     2341 gatcttgagc cagcgggaat caatctcatc gagcaatcgc cgggctttcg ataccgaatt
     2401 gacgacgtta tgcatttccg gctcgaacgc cagggtcact tcattccggt cggcgacctt
     2461 gatcgcagcg cggaccgaat ccaccagcgt ttcccaagtg gtgcgacgca cgttgtcggg
     2521 atgccacttc cacatatctt ccgggtcgag cgtccccgtg caaattgtga tgattcgggt
     2581 atccagccag cgacacgccg ccgccaggat atcgagtcgg cggaggttgt cctgaagtcg
     2641 cgccgtatcc ggatcgcaca ggttgaacgt accggagatc gcggccatcg ccatgccgcg
     2701 ctcttggaac aacctggcga tccaaacgca aaggtcttcg tcgatccgat ccggtaaggt
     2761 cggcagcccg gcgcacgtca tattgaactg cacatgagtg agaggatggc cggccacagc
     2821 gtcaagcgtc tcttcgagcg tcggccgcgg aaatgtcttg gcaaagatcc ccaggtgcat
     2881 cacacacctc cgttcacgta cttcgcggca agggcagcct gcccgtgaat cttcacggcc
     2941 tggagggccg tgccacactc atcatacatc tccactcacg tcggcgagcc gcacgtacgc
     3001 accagtctcc accgaccgcg cgatcgccac catcgcctgc acggcggcca agccatcgtc
     3061 agcactcgcg cccgcctggg gcaaaccatc caggatcgtg gctgcaaatc cctcgatctg
     3121 cagcttgtaa gtaaacgcgt cctcccccag aatacggtga aattggcgat ctcgcgccct
     3181 aaaacactcg acatcactcg ccttgtgata ccaagggaga tgaacgcggc cagtcacgct
     3241 gccatgctcg ccctgcacct ggaaaccttc ctgaaaatcg ccgcgtatcg ggatctggag
     3301 atcgaggtgg cccagcgagc cgtcggcaaa gtcgacggcg ataaaccagc agtacgcgcc
     3361 gaaccgctcg agcgctcggg cgcgcagtcc cacgatcggc cctcccaaaa aacgggccgt
     3421 atccaccaga tggctgccat gagtgaggag gtagtaccgc cgtcggtcgg ccttcggatc
     3481 tccttccggg cggcgtgcct gcgtgctcgt gaccgggatc ggctgcacgt tatccgtcat
     3541 cgtatagcga tagaccgagt cgtaatacca cgatttgagc gccatccgct ggcccatctc
     3601 acccgtgata aagtcgcgcg cgaaggcgac tcctggatcg aaccggcggt tgttgccgat
     3661 ctgcacgatc agcttggcgg ccgccgcctg atcgcgcaaa gcgcggcacg gttcgactgc
     3721 ggtccccagc ggtttctcga ccaagacgtg cttgccggcg gcgatcgccc tgagtgcggc
     3781 cggcacatga aattgatcgg cgatggcgat gacgatcgcc tcgacgcgcg ggtcggccag
     3841 catggcagcg tagtcggcat acgcgacctt gggccggtga acttcttgca tccgctcgcg
     3901 caggtcgtcg gccaggtcgc agatggcgta aagctcggcg tttttggcct tgcgacaagc
     3961 gtctaagtgc gcaaactggg cgatcggccc acagccgagt acgccgatcc gcaggcaacg
     4021 ctcgtctttg gcgatcatgc gactttcccc ggatagcgct gccgcaaggc ctcgccgagc
     4081 gcggccagct ttttgcgccg ggcgagggca gccaaagcgg ccaggtggtc ttccatgggg
     4141 acgtccttca gttcgcccga gcggtggacc tcgatcgcct gtagcatggc atccaggccg
     4201 atgcgcgggt ccggcttgcc ccctttgtcc ttgatcgtgt tgatggctga gcccgcaagg
     4261 aacaacacgt tctgggcgat tcccttcgac tcgaggtcct ggaggttcaa gatcaggttt
     4321 ccctgatcga gcccgccggc gcgggtgatc attgtcgggt tgatgccggc gaccggccgc
     4381 gtcaatgcct cttccgaggc ccgccactcg gcaccataag ggcggatgta gggggagccc
     4441 ggccgcaccg gggccgtctg gcgaaaatcg atgccgtcga gccgggccag gaaatcgatg
     4501 acctcgcgcc agataccgcg ggtcttgatg ccaatgcccg cattatggcc gtaaatggct
     4561 ggcggattgg cgaggtgttt ggtcgcgtcg cgcaccatgc gcaccgtccc gccggcgtag
     4621 gtctcactga acatgatccc cgtggctccg gcatcgacga ccgccagcac gaggtcgacg
     4681 atctggttgg gcccggccga aacgtgcggc gaaaagacga ggcccagccc gccgcgctgg
     4741 tcgcgcaccc gttcgatggc ggcgatagca cgcctggtcc gctcgacgac cggcgaataa
     4801 tcgaggtctg ggtaaaggtc ctcgtcttct ttgataaact tcagcgggaa agccacgtgc
     4861 a
//
LOCUS       DATAQL010000629         3897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4644444, whole genome shotgun sequence.
ACCESSION   DATAQL010000629 DATAQL010000000
VERSION     DATAQL010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4644444"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VHS97_10970"
     CDS             <1..640
                     /locus_tag="VHS97_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3448767.1"
                     /translation="TAEEVRQYDAMDHREVNRRFVTDFLAAHGPCRGGTILDVGTGTA
                     RIPIALASADSRAQLLALDLSSTMLAQAEINIASAGLTSRIRTQKGDAKALVEMFGES
                     AFEGVVSNTIIHHIPAPLPALETMARLVASGGTLMIRDLARPGSVGEITNLADIYAAG
                     ESPEARALFEASLHAALTLDEIQSLVRDLGLSPADVTMTSDRHWTWTWRKPL"
     gene            743..1618
                     /locus_tag="VHS97_10975"
     CDS             743..1618
                     /locus_tag="VHS97_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3448768.1"
                     /translation="MKPSEQIATAETLVREIEVRSWDAMVLRGRWWRRPAPRGAIIIS
                     HGFAEHGGCYRRVAEAFGARLEVDVIAVDYRGHGRSPGRRGVVRRFDDLVGDLASVVE
                     WTRRQLPDVPRFLLAHSNGGQVALRFVLDGNSSIDGMVVSNPAVRVSVPISPTKLRLG
                     RFLGMVAPWMTLRGDSRTDVLTRDPEIQEEHRADPLRHNRMSPPLFFGMVAGGEMLMA
                     RAAEIRVPLLMVLGGQDTVIDPGTSREFFDRLGSDDKTLLFYPKMLHEPLNDLGREQV
                     IEDMVRWLEPRLPSR"
     gene            complement(1632..2753)
                     /gene="dprA"
                     /locus_tag="VHS97_10980"
     CDS             complement(1632..2753)
                     /gene="dprA"
                     /locus_tag="VHS97_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX3448769.1"
                     /translation="MAETDADLRLIDILHLAMVPGVGPQTSRALLERFGSAGEVLDAT
                     RGQLCGVAGVGPKLAEKIVRARQDFDAAAELALCRRMEVNLISRGEPDYPPPLENIPD
                     PPGLLYVKGKIEPRDQLAIAVVGSRHCTPYGTRTAERLASALARTGFTVVSGLARGID
                     AAAHRSAIKAGGRTIGVLANGLASVYPPEHEDLARAVVEAGGLISEMPMRQGPLAGLF
                     TQRNRIISGLSLGVVVIEATPRSGSLSTAGHAMEQNREVFAVPGPVDSLASRGCHRLI
                     RDGARLVETVDDILEELGPLVSEVRTQPDEPPVRHPAELALSDSERSLLGHLDDQPTG
                     VDDLITRTGMTAGQVMATLSVLELKRLVRRQPGHRFVRV"
     gene            complement(3003..>3897)
                     /gene="aroB"
                     /locus_tag="VHS97_10985"
     CDS             complement(3003..>3897)
                     /gene="aroB"
                     /locus_tag="VHS97_10985"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX3448770.1"
                     /translation="AAITVLPTGESTKSLEQASRLYDELVKINAERHTLIVALGGGVI
                     GDLAGFVAATFARGLPLIMVPTTLLAQVDSSVGGKVGINHPDAKNIIGAFHQPAGVWI
                     DIKSLSTLPTRELRCGLAEVVKYGVILDAEFFVTLEQIAEDILRREHGALERVILRCC
                     QLKAEVVTQDEREETGLRAMLNFGHTVGHAIEATAGYDGRYKHGEAVAVGMVAECRLA
                     ERLGWVKGNVTDPLVRLLERFGLPTRAPELDANHLIDAMSRDKKNRRGKIRFVLPRSL
                     GKVELTDAAGVDDIRAVLTAS"
BASE COUNT          737 a         1210 c         1214 g          736 t
ORIGIN      
        1 aaccgccgag gaagtgcggc aatacgacgc gatggaccat agggaggtca atcgccgatt
       61 cgtcaccgat tttctggcag cgcacgggcc gtgccgggga ggcaccatac tcgatgtcgg
      121 tactggcacg gcccgcattc cgattgccct tgcgagcgca gacagccgcg cccaattgct
      181 ggcgctcgat ctgtcctcga ccatgctggc acaagccgag atcaatatcg cttcggccgg
      241 attgacgtcg cggatccgca cacaaaaggg cgatgccaag gcgttggtcg agatgttcgg
      301 cgagagcgcc ttcgaggggg ttgtctccaa cacgatcatt caccacatcc ccgccccttt
      361 gcccgcgctc gagaccatgg cccgtctcgt cgcatctggc ggcaccttga tgatcaggga
      421 tctggcgaga cccggatcgg tgggcgaaat cacaaacctg gccgacattt acgccgcggg
      481 tgaaagccca gaagcccgcg ctctgttcga ggcttcgctt catgccgccc ttactctcga
      541 cgagattcaa tcgctggtgc gcgatctggg gctctcgccg gccgacgtga caatgacatc
      601 ggatcggcac tggacctgga cctggcgcaa gccattgtga tcatctggag ccattaactg
      661 ctctcggcgc aggggtgacg ttcgattaaa tggttcctct ggaccgtcga gagccgtatc
      721 tcactccgag aatggacgaa agatgaagcc gagcgagcaa atagcgacgg cggagacgtt
      781 ggttcgagaa atcgaggtgc gctcttggga cgcaatggta ctccgcggcc gttggtggcg
      841 ccggccggca cctcggggcg ccatcatcat atcgcacggt tttgccgaac acggcggctg
      901 ctatcgccgg gtagccgaag ctttcggtgc caggctggag gtcgacgtga tcgccgtcga
      961 ctaccggggc cacgggcgga gcccaggccg acggggcgtg gtcaggcgat tcgatgattt
     1021 ggtaggcgat ctggcaagtg tggtggagtg gacgcggcgg cagttgccag acgtacctcg
     1081 gtttctcctg gcgcattcca atggcggaca agtcgcactg cgctttgtct tggacggaaa
     1141 ctcgtcgatc gacggcatgg tcgtgtcgaa cccggctgtg cgcgtgtcgg tgccgatctc
     1201 tcccaccaaa ctgagactcg gtcggtttct gggcatggtc gctccctgga tgaccttgag
     1261 gggagatagc cgcacggacg tgctgacgcg cgatccggag atccaggaag aacatcgggc
     1321 cgacccgtta cggcacaata ggatgagccc gcccctcttc tttgggatgg tcgcgggggg
     1381 cgagatgctg atggcccgcg cggccgaaat acgggttcct ttgctcatgg tgctcggcgg
     1441 acaagacacc gtgatcgacc cggggacttc gcgcgagttt ttcgaccgac tggggagcga
     1501 cgacaagacc ctgcttttct accccaagat gcttcacgaa cccttgaacg acctgggcag
     1561 ggagcaggtg atcgaggaca tggttcgctg gttagagccg cggctgccga gccggtgaca
     1621 gacgaggcct ttcagacccg cacgaatcga tgcccgggct gccggcgaac cagtctcttg
     1681 agctcgagaa cgctgagcgt ggccatgacc tggccggcgg tcattcccgt gcgggtgatc
     1741 aggtcatcaa cgccggtcgg ctgatcgtcg agatggccca gaagcgagcg ctcggagtca
     1801 gagagtgcta gctcggcggg atgtcgaacc ggaggctcgt cgggttgggt gcgaacctcg
     1861 ctgaccaacg gccccagctc ttccaggatg tcgtcgacgg tttcgaccag gcgggcaccg
     1921 tcgcggatca agcgatggca gcctcggctc gcgagactgt cgacaggccc gggaacggcg
     1981 aagacctcgc gattctgttc catggcgtgt ccggcggtgg aaagagaccc gctccgtggg
     2041 gtcgcctcga tgaccaccac accgagtgac aggccggaga tgatccgatt gcgctgcgtg
     2101 aacaagcctg ccaacggacc ttgccgcatc ggcatttcgc tgatcagacc gccggcctcg
     2161 accaccgctc gggccaggtc ctcgtgctcg ggggggtaga ccgatgccag gccgttggcg
     2221 aggacgccga tggtgcggcc cccggccttg atcgcgcttc tgtgcgcggc ggcgtcgata
     2281 cccctggcca gtcccgagac caccgtgaac cccgtcctcg cgagcgccga cgccagacgc
     2341 tcggcggttc gggtgccata gggcgtacaa tgccgcgaac cgaccaccgc gatggcaagc
     2401 tgatcgcgcg gttcgatctt tcctttgacg tacaagagcc caggtggatc ggggatgttc
     2461 tcgagcggcg gcgggtaatc tggctccccg cgcgaaatta agttgacctc catgcgccgg
     2521 cacaaagcca gctcggcggc agcgtcgaag tcctggcggg cgcgcacgat cttctccgcc
     2581 agcttgggtc ccacgcccgc gacgccacag agttgccccc gcgtggcatc caagacttcc
     2641 cctgccgacc cgaagcgctc gagcaaggca cggctggtct gggggccgac acccgggacc
     2701 atcgccagat ggagaatgtc gatcagtcgc agatcggcgt ctgtttcggc caagttttgc
     2761 ctccggtatt tggccgcgac tattgtcgaa aggagagcaa gattaagagg aagattaaga
     2821 ttaggaagag gattaggagc aagaggaaga ttaggattac gacaaagcga acgcggcgaa
     2881 tcgagtccta ctcttaatcc tgctcttact cttaatcatc ttcctaatct taatcttcat
     2941 cctcatctta atcttcagcg caagcgcccc cgcctacccg tcaatcgatg atgaacagtc
     3001 cactagctgg cagtgagcac ggcccggatg tcgtcaacac cggcggcatc ggtcagctca
     3061 accttgccga gcgagcgcgg cagcacgaac cggatcttgc cgcggcggtt tttcttatct
     3121 cgagacatcg catcaatcag gtgattggcg tcgagctccg gagccctggt cggcaatccg
     3181 aatcgctcga gcagccgtac caaggggtcg gtgacattgc cttttaccca gcccagacgt
     3241 tccgcgagcc ggcactcagc gaccattccc acagcgaccg cctcaccatg cttgtaccta
     3301 ccgtcgtacc cggccgtagc ctcgatggcg tgcccgaccg tatggccgaa attgagcatc
     3361 gcccgcagtc ccgtttcctc gcgctcgtcc tgggtgacaa cctcggcctt caactgacag
     3421 caccgcaata tgacccgctc gagcgcccca tgttcacgcc tgaggatgtc ctcggcgatt
     3481 tgctccagag tcacaaagaa ctcggcatcg aggatcacgc cgtatttgac cacctccgcc
     3541 aggccgcaac gcagctcgcg ggttggcaga gtgctcaggc ttttgatatc gatccagaca
     3601 cccgccggct gatgaaatgc gccgatgatg ttcttcgcgt cgggatgatt gattccgacc
     3661 ttgcccccaa ccgagctatc gacctgggcg agcagcgtag ttggtaccat gatgagtgga
     3721 agtccgcgtg cgaatgtggc cgccacaaag cccgccaggt cgccgatcac gcccccgccg
     3781 agtgcgacaa tcagcgtatg acgttcggca ttgatcttca ccagctcatc gtacagccgc
     3841 gacgcctgct cgagcgattt tgtcgattct cccgtgggca atacggtgat cgccgct
//
LOCUS       DATAQL010000630         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4492299, whole genome shotgun sequence.
ACCESSION   DATAQL010000630 DATAQL010000000
VERSION     DATAQL010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4492299"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VHS97_10990"
     CDS             <1..712
                     /locus_tag="VHS97_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448771.1"
                     /translation="RPFADKALKFKPHHPLASYVKARLLLSIGDDEENAVNILEPALD
                     LNEPNERVIDLLAELKMKAGDLDEAEKLYELARKDDPAHTKWIAGLARVHLRQKKTTQ
                     FLNDLAMIAANDADDIAVRKALAERHLGAGDAAAAEKWANECLYIDVYDPSVHLLLAE
                     AQALGKKYTSAIEEFETALALKPKKPNDLKVKLAQAQLAAGQRDAARATVDGVLKADP
                     EHPEAKALREKIEHPTSG"
     gene            1358..3535
                     /locus_tag="VHS97_10995"
     CDS             1358..3535
                     /locus_tag="VHS97_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448772.1"
                     /translation="MRVGFDASMALKSPNAFKIGTWTPVWVQLRGGSERYSGIMEVSV
                     ADDDGTPTVFSMPVEVGPNQSQRFTAYARPGSRQPEFTVRLLDQQGRRVGGASQETAM
                     PQPPESIMPDESLILTMGRPQGVETIAELPGFKAASGRPARGTSVEIVTARIDAQAGS
                     MPGRWYGYDAARAIVVDTGDRETVASLDALRGQPLVDWVERGGHLIVAVGANWQAVRD
                     SVLAPILPGLPSGQEKVASLEALDTFAGSNKSITPPGTPPTMITKLEEIDERRGTVLS
                     MMSGLPLVVRGTHGFGRVTLIALDVDQKPFSDWVDRSLFWVRAIELRRPRGDQTAGGP
                     NMGGGSRFYQYGASDMSTQLRVALEQFPGVKLIPFGWVAFFIFLYILLIGPGDYFFLK
                     KVLKRMELTWITFPTIVVTVSLVAYYAAYILKGNDLLVNKVDVVDIDQAAGLGRGNTW
                     LSLFSPQNRDYTIRVIPQPLERDAPPAAVTDSSSGPVRPPTGTEVVTSWYSAPEDQFG
                     AMGNSGRRFSFAASGYSYQPTAGVEQLENVRIPIWSTKCISSRWFGPAAPLVDSDLQS
                     VGTDRLAGIVTNRQSVPLEDAILAFDKHVYLLGTLAPGATVRVELTSDRNLSGLLKEK
                     QRNFISDQSWNRDRPINRADLMLSAMFHDSESTLASDHVLANDPLHDLDLTGLLALKR
                     PMLVARINRPGAQLRLDNVPSPPKMDQLTMVRLILPLKQKAKS"
     gene            3699..>4255
                     /locus_tag="VHS97_11000"
     CDS             3699..>4255
                     /locus_tag="VHS97_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3448773.1"
                     /translation="MIETNDLTKMYGELYALNRLNLTLQQGDVYGFIGPNGAGKTTTM
                     RILATLLNPSWGEATVCGYSIYTGAKEIRRVIGYMPDFFGVYDDMKVIEYLEFFASAY
                     RIKGAARRKICEEVLELVDLTYKRDALVTSLSRGMTQRLGLARTLLHDPQVLLLDEPA
                     SGLDPRARIEMRALLKELRNMGKTIL"
BASE COUNT          887 a         1277 c         1287 g          804 t
ORIGIN      
        1 gaggccgttt gcggataagg cgctcaagtt caagcctcat cacccgctgg ccagttacgt
       61 caaggcgcgg ctgttgttgt cgatcggtga cgacgaagaa aatgccgtga acatcctcga
      121 gccggcgctc gacctcaatg agcccaacga gcgggtgatc gatctattgg ccgagctcaa
      181 gatgaaggcc ggcgatctcg acgaagccga aaaactctat gagctggccc gcaaggacga
      241 cccggcacat accaagtgga tcgcaggtct ggcgcgggtg catctccgtc agaagaagac
      301 aacccagttc ttgaacgacc tggcgatgat cgcggccaac gacgccgacg atatcgcggt
      361 gcgcaaggcg ctcgcggagc gccacctcgg cgctggtgat gccgcagcgg ccgaaaaatg
      421 ggccaacgaa tgtctgtaca tcgacgttta tgacccctcg gttcaccttc tcctggccga
      481 ggcccaagcc ctcggtaaga agtacaccag cgcgatcgag gaattcgaga ccgcgcttgc
      541 gctcaagcca aaaaaaccta acgatctgaa agtcaagctt gcccaggccc agctggccgc
      601 gggccagcgc gacgccgcca gggccactgt cgacggcgtg ctcaaggccg acccggaaca
      661 tcccgaggcc aaggcgctcc gcgagaaaat cgaacaccct acgagcggat gagcacgccc
      721 gcgagccgag gaaatcccac gccggtgtga caacgcggtc atgccacgcc gatcgtaccc
      781 ccgcacgaaa gcaacgggcg aaccaaacgg ctcatggccg aaggcaatga caacgtcgat
      841 cagttcagcg cgcgaatggc tctacaggga accccctgtc aaacagctgc gacagcagcc
      901 ggctgaggtc accgcgctcg cgtaaaggcg ccaggtcggg gtcgctgatg atagccagtt
      961 gagaatccta agttcaattc ggtggaggag aggatgaccc cgaggtaatt cacctcgcgg
     1021 tcgcctgcgt tcaccaggta agatgtggtc atgatcgaaa catcagggtc gtcaggtgcc
     1081 ttgacactgt ccggcgtgca gcggatgatc ttacaaatgt acggccagaa agatgccgcc
     1141 cgtggtgatg cggccacgtt tctctggctg aattcctcga cttcaatagc tttcaaacat
     1201 gattaatcca aatacagcgg cgggatggcg ccaaattctt gaccggcggc gtgtgtgtgt
     1261 gtgccgcggg cagttcgcgg tggtgacact cctaatgttg gtcggcgcac cgaccgcggc
     1321 gttggcgcaa agatcgcggg gagtcgaagt tgaaaacatg agggttggct tcgacgcatc
     1381 catggctctc aagtcgccaa acgcgttcaa gatcggtacc tggactccgg tatgggtcca
     1441 gcttagaggg gggagcgagc gttacagcgg catcatggag gtgagcgtcg ccgatgatga
     1501 tggaacgccg acagttttca gcatgccagt cgaggtgggg ccgaaccaga gccaacggtt
     1561 cacggcgtat gcgcgcccgg gctctcgtca gccggagttc acagtccggc tgctcgatca
     1621 acagggacga agggttggcg gagcgtcgca agaaaccgcg atgccgcagc cgcccgagtc
     1681 gatcatgccc gacgaatcgt tgattctgac catgggccga ccgcagggag tggaaacaat
     1741 cgcggaactg cctggtttta aagctgcaag tgggcgaccg gcccgcggca ccagcgtcga
     1801 aatcgtgacg gcacgaattg acgctcaagc cggctcgatg cccggccgct ggtacggcta
     1861 cgacgcggca agagccattg tcgtggatac gggcgaccgc gagacggtgg catcgctcga
     1921 cgccttgcgg ggccagccgc tggtcgactg ggtcgagcga ggtggtcatc tcattgtcgc
     1981 ggtcggcgcc aactggcaag cggtgcgtga cagcgtgctg gcgccgatcc ttccggggtt
     2041 acccagcggt caagaaaagg tggcttcact ggaagcgctc gataccttcg ctggttcgaa
     2101 taagtcgatc actccgcccg gcactccacc gacgatgatt accaagctcg aggaaatcga
     2161 cgagcgcaga ggcacggtcc tctccatgat gtcgggcttg ccgctggtcg tgcggggtac
     2221 acatggtttc gggcgcgtga cgttaattgc cctggatgtc gaccagaagc cattttccga
     2281 ttgggtcgac cgctcattgt tctgggtcag ggccatcgaa ctgaggcgcc cacgcggcga
     2341 ccagacggca ggtggcccca acatgggcgg cgggtcgcgg ttctatcaat acggcgcctc
     2401 cgacatgtcg acccagctac gcgtcgccct cgagcagttt ccgggggtca agctgatccc
     2461 attcggctgg gtcgcctttt ttatcttcct ctacattctc ttgatcggac cgggcgacta
     2521 cttctttctc aagaaggtgc tcaagcggat ggagctgacc tggatcacct tccccacaat
     2581 cgtcgtgacc gtgagcctgg tggcatatta cgcagcttac atcctcaagg gaaacgacct
     2641 cttggtgaac aaggtggatg tcgtcgacat tgaccaggca gccggtctcg gtcgcggcaa
     2701 tacttggctc agccttttca gccctcaaaa tcgcgactac acgatccggg tcattcccca
     2761 gcccttggag cgcgacgcgc cacccgccgc ggttactgac tcatcgagcg ggccggtccg
     2821 accgccgacc ggaaccgaag tcgtgacgag ttggtacagt gccccggaag atcagtttgg
     2881 agccatgggc aattccggcc gccgtttcag cttcgcggcc agcggctact cgtatcaacc
     2941 cactgcggga gtcgagcaac tcgaaaacgt ccggatcccg atctggagta cgaagtgcat
     3001 ctcgtcgcgc tggtttggtc cggccgcgcc gctggtcgat tcggatctac agtcggtggg
     3061 gaccgatcgc ttggccggca tcgtaaccaa tcgtcagtcg gtcccgttgg aggacgcgat
     3121 cctggcgttc gacaagcatg tctatttgct gggtactctg gcacccgggg ccaccgtgcg
     3181 ggtcgagctc accagcgacc gtaacctgtc agggctctta aaggaaaaac agcgtaactt
     3241 catctccgac cagtcctgga atcgcgacag gccaatcaac cgggctgatt tgatgctgtc
     3301 ggcaatgttt cacgatagtg agtcgacgct ggccagcgat catgtcctcg ccaacgatcc
     3361 gctgcacgac ctcgatctga cggggctgct cgcgctcaaa aggccaatgc tcgttgcccg
     3421 gatcaatcgt ccgggcgctc aactcagatt ggataacgtg ccttcgcctc ccaagatgga
     3481 ccagttgacc atggtacgcc tcatcttgcc tttgaagcag aaagccaagt catagggaat
     3541 gccacgcgat cgattggttc agggggtcag ccccaatacc gttgatctac gtatcactta
     3601 ttagtccgaa gtcagttctg tctccctcct cccgtgtggg ggagggttgg ggtgggggga
     3661 ggcaggtgcc tcgccctcaa ttaccaaggc gcaacacgat gatcgaaaca aacgatttga
     3721 ccaaaatgta tggcgagctg tacgccctca accgtctcaa cctgacgctc cagcagggag
     3781 acgtctacgg ctttattgga cccaacggcg cgggcaaaac aacgacgatg cggatcctgg
     3841 ccacgctctt gaacccttcc tggggcgagg cgactgtctg cggctactcg atctacaccg
     3901 gcgccaagga aatccggcgc gtgatcggct atatgcccga tttcttcggc gtctacgatg
     3961 atatgaaagt catcgagtat cttgaatttt ttgcctctgc ctaccggatc aaaggagcgg
     4021 cgcggcgaaa gatctgtgaa gaggtgctcg agctcgtcga cctgacttac aaacgagacg
     4081 cgctggtgac cagcctctcc cgcggcatga cccagcgcct cggcctggcg cgtaccctgc
     4141 ttcacgaccc ccaggtcctt ctcctggatg agccggcctc gggtctcgac ccgcgcgccc
     4201 gcatcgagat gcgtgccctg ctcaaagagc tacgcaacat gggcaagacg atcct
//
LOCUS       DATAQL010000631         4655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2813040, whole genome shotgun sequence.
ACCESSION   DATAQL010000631 DATAQL010000000
VERSION     DATAQL010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2813040"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHS97_11005"
     CDS             <1..199
                     /locus_tag="VHS97_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448774.1"
                     /translation="HWLNVSYSVWFNRKYNRVGPLFQGRFKAILHETTEALMINRYIH
                     LNPVRIAALVMKIHVEHGASR"
     gene            complement(627..2381)
                     /locus_tag="VHS97_11010"
     CDS             complement(627..2381)
                     /locus_tag="VHS97_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3448775.1"
                     /translation="MPSAPQHSEGRFWPDGARLTFRADDGLEIVGEVRGQGDTALIFL
                     HGWCGDREYWKHQVEVFAADYRVVALDQAGHGESGKGRKAWTADGLAGDVEAVATALG
                     LKRVILVGHSMGGSVALLAAKRMPGTVIAVIGVDTLQNAELKLPEEVRKSILDGVEKD
                     FKGTVRVTIAGLLPEKADAELKTWLQTKAEGQDPKTALTLMRDLFGLDTRKLLKEAKV
                     PVRCINSAGGYKFFTPTAVETNKKYADFGAVTIDGVGHHPMLEKPDEFNRKLRDVLKE
                     LRPRSEPASPGTGAGRRNLVVLRSCLLITLGVVGCQQKPALQAPPPPTVVVAKPVNKQ
                     IVEWQYFTAQTQAVDTVTITPRVTGYIDNISFKEGDVVDVGDLLYVIDPRPYQATLHQ
                     AKGQLEQALAQQKLDNANLERAKDLLAKKVIAQQVFDTTAAQKYVADAQAVASQAAVE
                     SAQLNLNFTHIRSPIRGRISAQLVNRGNLVQANSTRLTTIVTIDPIYAYFNVDEASVI
                     RFQKAIEAGQFVGPRQATLPIWLQLDTEIGYPHQGVIDIVDNSFDPSTSTLRLRGRFP
                     NQQGYYLRHCPNGRIPEI"
     gene            complement(2411..2551)
                     /locus_tag="VHS97_11015"
     CDS             complement(2411..2551)
                     /locus_tag="VHS97_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3448776.1"
                     /translation="MANSRRPMVARLKVGRLLLGGDAAHIHSPAGAQGMNTGIQDMIN
                     LC"
     gene            complement(2574..2906)
                     /locus_tag="VHS97_11020"
     CDS             complement(2574..2906)
                     /locus_tag="VHS97_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3448777.1"
                     /translation="MAQASAFRLDFTWIDSRYRYLLFISQAETERILRTAVEKQGVRT
                     ERGVELVGVAQDVPSPDPNPVRVVLRHAASRLEQAQAPWLIGAEGAHSTVWATLVLPF
                     EGKTRDEQ"
     gene            complement(2863..3105)
                     /locus_tag="VHS97_11025"
     CDS             complement(2863..3105)
                     /locus_tag="VHS97_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3448778.1"
                     /translation="MLDRVLIVDAGPTGLTAAVRIIDKQAAPPTTSRAVGVQARTLEL
                     LELRGVENEMVRLGNPGLAASFYGAGKRVSPRLHVD"
     gene            complement(3389..3616)
                     /locus_tag="VHS97_11030"
     CDS             complement(3389..3616)
                     /locus_tag="VHS97_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3448779.1"
                     /translation="MIERSVILSHGPALQVPIGDLQSAVTQAPEPTAAAVTLAEAQRE
                     HILGALRETGWVVAGPKGAATRLGMKRSTLQ"
     gene            complement(3613..>4655)
                     /locus_tag="VHS97_11035"
     CDS             complement(3613..>4655)
                     /locus_tag="VHS97_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3448780.1"
                     /translation="LPLEEDPAGWVWQTQQARIFSNVPEEKRWPRALERAKWYGVQSS
                     CNLPLTTARRRLGILVFACKQPSAYETADLSYLQLVANQVAVSVENALAFQEIEALRD
                     QLSKANAYLEEEVRTEHNFGEIIGDSAALRRVLQEVETVAPTGSTVLIRGETGTGKEL
                     IARAVHDLSPRQGRTFVKLNCAAIPTGLLESELFGHEKGAFTGAIMQKSGRFELAHQG
                     TLFLDEVGDIPQELQPKLLRVLQEQEFEHLGSTRTIKVDVRLVAAMNRDLARMVADGR
                     FRSDLYYRLNVFPIALPPLRERREDIPPLVRHFTQRFVRRMGRRIEAIPAAIMDALAS
                     YPGRGTYASCKT"
BASE COUNT          815 a         1452 c         1443 g          945 t
ORIGIN      
        1 gcactggctg aatgtcagct acagtgtttg gttcaatcga aaatacaacc gcgtcgggcc
       61 tctatttcag ggacgcttca aggcgatcct ccatgaaact acagaggcac tcatgatcaa
      121 ccgctacatt catttgaatc cggtaaggat tgcagccctt gtgatgaaaa ttcacgtcga
      181 acacggcgcg agccgatagt ccctattcgc aggtgtctgt cccagtgtct gtccctattc
      241 gcaggtgtct acggttgatc gggtagttcc ggtcggcaat gacgtacgtg cgttcggaac
      301 atggagcatg ttactcgcaa ccttagttaa gcctgcaaat acaccttccg agccacaggc
      361 atcaccgcct acctcaaaaa tggcggacgg cttgaaagtg gcccagcaaa tcgccgccca
      421 cgagtcatct cgcaccactg ggctttatga tcgacgcggc aatgatgtta gcttggacga
      481 ggtggagcgg atcgcaattt gatgaacgtt ttactcagga tctttccggt ttaggcaaga
      541 tggacgtgtt cgcggttgta gttccgttta gctctgggga ccgtgagacg gccggcttcg
      601 gcccgaagaa tttcattagt atcccctcat atttcgggga tccggccatt cggacagtgc
      661 cgaagatagt agccttgttg attcgggaac cggcctcgca accgcagggt acttgtcgac
      721 gggtcaaaac tgttgtcgac gatgtcaatg accccttggt gcgggtatcc aatttccgtg
      781 tctaactgaa gccaaattgg gagagtggct tgcctcgggc ccacaaattg cccagcttca
      841 atggctttct gaaaccggat aaccgacgct tcgtccacat taaagtaggc gtagatcgga
      901 tcaattgtca cgatcgtcgt caatcgggtt gaattcgcct gcaccaggtt tccccgattc
      961 accagctgcg cgctgatccg gccacgaatc ggcgaacgaa tatgtgtgaa attaagattg
     1021 agttgcgcgg attcgactgc ggcttggctg gcgactgcct gagcgtccgc tacgtatttt
     1081 tgagccgcag tggtatcgaa aacctgctgg gcaatcacct tcttcgcgag caaatctttg
     1141 gctcgctcca agttcgcgtt gtccagcttc tgttgcgcga gagcctgttc taattgtccc
     1201 tttgcttgat gcaatgttgc ttggtaagga cgggggtcaa tcacatagag gagatcaccc
     1261 acatccacaa cgtccccttc cttgaaagag atgttgtcga tatagccggt aaccctgggc
     1321 gtgattgtta ccgtatcaac ggcttgcgtc tgggccgtga aatactgcca ctcaacgatc
     1381 tgcttgttga ccggttttgc caccactacg gtcggcggcg gtggagcctg caaggcgggc
     1441 ttctgttggc atccaacaac tcccagggtt ataaggagac agctcctcag gacaacaagg
     1501 tttcttcgcc ccgcccccgt gccgggcgag gcgggctcac ttcttggtcg caactccttg
     1561 agcacgtccc ggagtttgcg gttgaactcg tccggcttct ccagcatcgg gtggtggccg
     1621 acgccgtcga tggtgaccgc cccgaagtcg gcgtacttct tgttggtctc gacggccgtg
     1681 ggggtgaaga acttgtatcc gccggcggag ttgatgcacc ggaccggcac cttggcctcc
     1741 ttcaacagct tcctcgtgtc gaggccgaag aggtcgcgca tgagggtcag cgccgtcttc
     1801 gggtcctgac cttcagcctt ggtctggagc cacgtcttca gctcggcgtc cgccttctcc
     1861 gggagcaacc cggcgatcgt cacacggacc gtccccttga aatccttctc gacgccgtcg
     1921 aggatcgact tgcgcacctc ctcgggcagt ttgagctcgg cgttctgcag ggtatcgacg
     1981 ccaatcacgg cgatgaccgt ccccggcatc cgcttggcgg ccagcagcgc gaccgacccg
     2041 cccatcgagt ggccgacgag gataacgcgc ttcaggccca gcgccgtggc caccgcctcg
     2101 acgtcgccgg ccaggccgtc ggcggtccac gccttgcggc ccttgccgga ctcgccgtgc
     2161 ccggcctggt cgagggcgac gacgcggtaa tcggcggcga acacctcgac ctggtgcttc
     2221 cagtactcgc ggtcgccgca ccagccgtgc aggaagatga gggccgtgtc gccctggccg
     2281 cggacctcgc cgacgatctc cagcccgtcg tcggctcgga aggtcaggcg cgcgccatcc
     2341 ggccagaaac gtccctcaga gtgctggggt gctgatggca tagaatcctc ctttcagtag
     2401 gcgacagaga ttagcacagg ttgatcatgt cctggatgcc cgtgttcatg ccctgagcgc
     2461 cggccgggct gtggatgtgg gccgcgtccc cgccgagcag caggcggcca accttcaggc
     2521 gtgccaccat aggtctccgc gagttcgcca tcgatgtgca ggtcgcccag ggcctactgt
     2581 tcgtcgcgtg tcttcccctc gaacggaagg acgagggtgg cccagacggt gctgtgcgcg
     2641 ccttcggcgc cgatgagcca gggcgcttgc gcctgctcca gccggctggc cgcgtggcgc
     2701 agcacgaccc tgaccgggtt cgggtcgggg gagggcacgt cctgcgccac cccaaccagc
     2761 tcgacgccgc gctcggtccg gactccctgc ttttcgaccg ccgtgcgcag gatgcgttcg
     2821 gtctcggcct gcgaaatgaa caggaggtag cgatagcggc tgtcaatcca cgtgaagtcg
     2881 aggcgaaacg cgcttgcctg cgccataaaa actggcggca aggccggggt tgccaagccg
     2941 gaccatctcg ttctccacgc cgcgcagctc gagcaactcc agcgtgcgcg cctgcacccc
     3001 gacggcacgg gacgtcgtcg ggggagcggc ctgcttgtcg atgatgcgga cggcggccgt
     3061 cagtccggtt ggcccggcgt ctacaatcaa tacacgatcc agcatacgat gtctcctcag
     3121 ttaaatggga tgggctggtt tcaggatcgc cctccggcgc ctcgctcctg cccttacctg
     3181 cttcctctaa aagcacaggc catgccggtg aggcggactt tggcgcttcg ctccataacg
     3241 caagcactag gaaggacttg tggatcggat gggtcgagcc ggcttgcggc gaggactgga
     3301 agtgccaggt aggaagtgcc gccaggccgg tacagctgcc tgcggaccgg cactactggg
     3361 gcccggagat tccgagtttt ctcattttct actgcaaggt cgaacgcttc atccccaggc
     3421 gggttgcagc ccccttcggg ccggccacca cccagccggt ctcccgcagg gcgccgagga
     3481 tgtgctcccg ctgggcctcg gccagtgtca ccgccgcggc ggtcggctct ggggcctgcg
     3541 tgacagcgga ctgcaagtcg ccgatgggga cctgcagcgc cggaccatgg gagaggatga
     3601 ccgagcgctc gatcacgttt tgcagctcgc gtacgttccc cggccagggt agctcgccag
     3661 ggcgtccatg atcgcggccg ggatggcctc gatccgccgg cccatccgcc ggacgaaccg
     3721 ctgggtgaaa tggcgcacca gcggcgggat gtcctcgcgg cgctcgcgca ggggcggcag
     3781 cgcgatcggg aacacgttca ggcggtagta gaggtcgctg cggaagcggc cgtcggccac
     3841 catccgggcc aggtctcggt tcatggccgc caccagtcgc acgtccacct tgatggtcct
     3901 ggtgctgccc aggtgttcga actcctgctc ctgcaacacg cgcagcaact tgggctgtaa
     3961 ctcctgcggg atgtcgccta cttcgtcgag gaagagtgtt ccctggtgcg ccagctcgaa
     4021 gcgcccgctc ttttgcatga tggcgccggt gaaggcgccc ttctcgtggc cgaacaactc
     4081 gctctccagc agcccggtgg ggatagccgc acaattcagt ttgacgaacg tgcggccctg
     4141 gcgcgggctc aggtcgtgaa cggcgcgggc gatgagctcc ttgcccgtgc cggtttcgcc
     4201 ccgaatgagc accgtggagc cggtaggggc gaccgtctcc acctcctgga gaacgcggcg
     4261 gagggcggcg ctatccccga tgatctcccc gaagttgtgc tcggtgcgga cttcctcctc
     4321 caggtaggcg ttcgctttcg agagttggtc tctcagcgcc tcgatctcct ggaaggccag
     4381 ggcgttctca acagacacag cgacttggtt ggcaacgagc tgcaggtagc tcaaatccgc
     4441 tgtctcatag gcggagggct gcttgcacgc gaaaaccagg atgcccagcc gccgccgggc
     4501 tgtagtgagc ggcaaattgc aggaactctg gaccccatac cacttcgctc gttcgagggc
     4561 ccgcggccag cgcttttcct cgggcacgtt cgagaagatc cgcgcttgct gggtctgcca
     4621 gacccaccct gccgggtcct cttcaagagg aagaa
//
LOCUS       DATAQL010000632        15093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4492541, whole genome shotgun sequence.
ACCESSION   DATAQL010000632 DATAQL010000000
VERSION     DATAQL010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 15093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15093
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4492541"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VHS97_11040"
     CDS             <1..134
                     /locus_tag="VHS97_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEX3448781.1"
                     /translation="RHANFIVAHPGAKASDVLELIDRIRQRVWQQFGYELELQIQVW"
     gene            161..1255
                     /locus_tag="VHS97_11045"
     CDS             161..1255
                     /locus_tag="VHS97_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448782.1"
                     /translation="MEHREPPRIGPGDRVFDASLEEPAGRFRLRPLIAGRDARQFDSV
                     KVGVVLLVGLAVAITLVFLGSWARRATLDWLAVQAPYQIPFKQIHLVNPPPPWFLGGK
                     DALLEGVRKSAGEPETISVLQVSPEQLATVFKKYAWVEGVKVAYPPGQIDVELRYHQP
                     VAWVQLRLADRIVIDEKGNILPAEDVDVSQLRKLIMIIGAGLAGPADRQPGVVWKVRD
                     SNGLEQIDEGIVAAAKLASFLLEEPQKGDAQRSNALRILKINVTDSSSRGLFVYNAED
                     ITILWGKAPGDESPGKPTAAEKWAMLRRWQETEKARFLADGDFWAFSNDRLRFVCPSR
                     HPTRHQPFIKKLYEEPGDPSDLHSKPPGSG"
     gene            1390..3342
                     /locus_tag="VHS97_11050"
     CDS             1390..3342
                     /locus_tag="VHS97_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX3448783.1"
                     /translation="MASRLGPNWLNRFSQLAGPTHAGRLGRYAILVDRINALEPLFEV
                     LSPEQLQSKAHDLRLRARQGDSLKSLLVEAFALVREAAKRAIGQRQFDVQIVGSIAIH
                     NRCIAELETGEGKTLVATMPAFLNALPAKGVHIVTVNDYLAHRDADWMGPIYRLLGLT
                     VGCIQTGQSDAERRQAYACDITYGTAKELGFDFLRDELKKLQLGGDAHRKTFEEVYLG
                     RGGHYEAQMPVQRTHAYAIVDEADSILIDEARTPLIIGANNQPTQEEAAAYYGADQLA
                     ATLVRIKDYKYDPLERKAELTATGRRKVQAVAANPAFVSLTVDGLYEYVERSLRAQIA
                     YLKDRDYVTVDGEVVIVDEFTGRMMPGRQWQDGLHQSIQAKERLEITLETITAARVTV
                     QDFFKRYNKLAGMTGTASSDAAELRRIYKVGVFKVPTNRPGRRVWLPDRVFSNSEERF
                     QAVADQIAEWNKLGVPVLVGTRSIEKSERLSALLEAQGIEHQILNAKNHEIEAQIVAQ
                     AGQNGRVTVATNMAGRGTDIKLGEGVAELGGLHVIGTERHEARRIDRQLSGRCARQGD
                     PGLAQFFICLDDEIIEAFGEKSAARIRKRLGGTGELTSVRMRRFFVAAQRKKERQHFR
                     DRKLLMHYEKQRAEMRKNMGLNPVLG"
     gene            complement(3360..4364)
                     /locus_tag="VHS97_11055"
     CDS             complement(3360..4364)
                     /locus_tag="VHS97_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX3448784.1"
                     /translation="MYRSLSLSSARGILPISLISRRSWLKRVLLAAAVGPAMPRVGLS
                     MQSKVDASDADEIAAVQALAKKAGLVPFKDSHTDHFLGLGDAPEAFRKTALGICESLS
                     AAFLPHFRQKGFNLAMPAQRLTVITLKDKESYGAFLGEDPGKMVGGHYDLDTNRLVMF
                     DFRPTSDEIDVQADPQRVNLLALVHETTHLLCFNTGLLSRQTDVPDWVSEGLATYVEL
                     WQRKKPATSIGTVNRPWLQCLRTSRSWIVIPDLVADDKTFWDEKTQQLSYGESWLLVH
                     YLMKTDAQFPKFRAYLAELPPQGQGTAANRVALAEKHLGSLKKLDREVADRYLKRLAR
                     "
     gene            4887..5699
                     /locus_tag="VHS97_11060"
     CDS             4887..5699
                     /locus_tag="VHS97_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3448785.1"
                     /translation="MLRADTHNLSFSTRTRLLIKYAMYPGTNWISRDKAKIVKMFLRG
                     THDRPVRTLDCGCGNAYFAHQAVLRGSRCTGITIHEWEKHNCEEMRSFLNVSHGDLEF
                     RVANLESLAADPLEHGQYDQVLFLDVIEHILEARSALRQIHALTAQDGFVYITTPNRD
                     WQGNADRIRVTRDEDGWHLRNGYTFEQLETLLEETGFEPVDRLRFGTAGSTLVTWIQH
                     KLFQRWIDPLTVAFFPLLKILAAVLSPWRDTHTIFVLARKKRGSACSPDRAD"
     gene            5785..6774
                     /locus_tag="VHS97_11065"
     CDS             5785..6774
                     /locus_tag="VHS97_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3448786.1"
                     /translation="MSDVRYSPPFFVGIDLGGTNIKSGVVDNEGRSLSSVSIETHAEK
                     GPVAGVDSLVAAANKAVEASGIKWDRIAAVGLGSPGTMDLEAGMLLDPPNLPGWDNFP
                     IRQRLAERLQKPTALQNDANAASYGEFWVGAGKGGRSLVMFTLGTGIGCGIVVDGMII
                     EGRHSHGAECGHIIIEMENGRQCSCGAYGHLEGYASATALVKRAHEALGADTKPSRLR
                     SLEHDELTSRAIAEAADSGDELARRLMRETAHYLAVGAVSLMHTIDPDIVLFGGGMIA
                     AGQPFLEAIRADIRAMAFPVPAAKTRVEYAQLGGEAGFIGAAGCARLAFHRPD"
     gene            complement(6725..8506)
                     /gene="recJ"
                     /locus_tag="VHS97_11070"
     CDS             complement(6725..8506)
                     /gene="recJ"
                     /locus_tag="VHS97_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX3448787.1"
                     /translation="MNARWRIRPHNPADIASLSRRAGISPLVAQLLLNRGIDQPDRAR
                     AFLDARMVSLHDPALIPGMVEAADRIVRAIRDDRKIVIYGDYDVDGVCGTSILWQCLR
                     LAGSAHAEYYIPHRVEEGYGVNADALRKLAAENKAELVVTVDCGISAVREADLARELG
                     IELIITDHHTIGWTLPRADTIVHPRLPGGPEFEADLCGAAVAFKLAWQLCKSFGDGKR
                     ASPVLREFLVRALGLVALATIADMVPLSDENRVMVRHGLSGISTGPSPGLVALLEVSG
                     ARDRRKLTSGTIGFGLAPRINAAGRLERAMMAVEMLTTADLPRAREIAGELDRCNARR
                     QEVERQIYEEAKAIIDSEGGLGDRRAIVLGRPGWHAGVIGIVAGRLAESFHRPVVIIS
                     LGTEISQGSARSIPGFDLYEALKDCSEGLLAYGGHAAAAGLKLHADHFPVFARRFEER
                     CKASLSSEQLERVLSIDAEVPLGILSLGVVEEIEKLEPYGISNPRPLLLASAVEVAGA
                     PRPVGEKKNHLQLRLKQGNHLLKAIAWNLAEKSRDLAAGARCSIVFSPSINEWNEHRE
                     VQLEVKDIVIDPNPAGETPTWRSLLHR"
     gene            complement(8550..9617)
                     /gene="corA"
                     /locus_tag="VHS97_11075"
     CDS             complement(8550..9617)
                     /gene="corA"
                     /locus_tag="VHS97_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX3448788.1"
                     /translation="MSGLTPVADPLPRAAQEPAENESVIRLSYRDGAGVFHAEWPYAQ
                     IEQAINDDKGILWVDIQGPDEHAGDELENWLCTQFHFHHLAVEDALRESHIPKVDDWG
                     DYLYIVFHVARIVPETEALELQELDVFLGKNYLVTYHTAPLPILDRDRESIVRDPRDR
                     LRNGPDHLLFRFLELAIDQSLEAIEDLDEQVDEIQNAVIDNATPTNLKKIFRIKRSAI
                     QLHKILSPQREVLNRLARDPYKPIQPKHRVYFRDLYDHVVRIHDISESLRDLISGTLE
                     TYLSVVSNRTNDIMKTLTIVTVMFMPMSFLVGFFGMNFFGETLAFKTEFPKGLLFWGA
                     CAIMTLSPCFIWIYARRRKWF"
     gene            complement(9614..10084)
                     /locus_tag="VHS97_11080"
     CDS             complement(9614..10084)
                     /locus_tag="VHS97_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448789.1"
                     /translation="MTDRPRLRTRLDLFVLLWISVAAAGCSEELGPERFLVARISGVV
                     REGSRPVAGGWIEFIPVDGTVGNLCSAKLGPDGSFVADKVAIGVNLIRLVNAPLNTPG
                     AIRVVGAYQSPIRREIAAGPTEPLVIDVVEELIRHQSSLGLPAAPDSRKTGGAR"
     gene            complement(10104..11222)
                     /locus_tag="VHS97_11085"
     CDS             complement(10104..11222)
                     /locus_tag="VHS97_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448790.1"
                     /translation="MAAKLTDKSNVQALVEGNQSLSAPATRGIQEATTALLEPLFGAR
                     SIKNLPFLQIYHRELRNSLTPLLKNHLIPRVQAMIILGECGAADFLPLYEAQIKDPNQ
                     TTWVKLWALEGIVNVVEEGGGRLTAQDQIVAAKTVSDYLDNEDGIPWPVQLRAMEALS
                     AMRQGFEPNRPKKADMANAAMSLLADGSAKFEVRSEAARALGLMQISSSVPKYNYRLV
                     VHSAAQLAAELGNRIASGYATKIQDRVNQDRAKYLTALLIGPVYQAFDGVPGARDSGL
                     VHVAAGDSAAYVQSVFDLVKPVARAAVDLTAAGSRQIPDRQKELVARVAGLKDFLEKN
                     APADRHLVPEGTEFPVALVPEVQLKAPAAPLAGKVKNR"
     gene            complement(11319..11684)
                     /locus_tag="VHS97_11090"
     CDS             complement(11319..11684)
                     /locus_tag="VHS97_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448791.1"
                     /translation="MLKALLPLRKWEFPRVWHGVVLSLSLVLFLFQMSARAQTQPDSK
                     KGSPDASKTPKAGSAATGGPAAKATTGKAAPAPDVEAPAPAANADPSKTRKIAPVEIF
                     RDKRAEAWLDLAKLKPVTA"
     gene            12312..13109
                     /locus_tag="VHS97_11095"
     CDS             12312..13109
                     /locus_tag="VHS97_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3448792.1"
                     /translation="MIERRYVFPGVLEMNYQAQRRMGVNIYLVDGGTEFALIDVGFLD
                     ELPDVLELIRQMDFSLSACKMIVATHADVDHTQGLTRARDVLKCEVAAHPKSVAAIEE
                     GDEILTYARIDAQGIHIPMPRCKVDKVIGEGDELQIGDKTLEVWSTPGHTAGQLALRM
                     GRLLFSGDNIFRDGGVGVIDAHHGSNLLEFVASLKRILASDVDYLLPSHGPIFRKDDA
                     LLRATIARLEAYTHMADFGTCAVDWPLMDQWEEELARGDLRFDGPHR"
     gene            13262..13852
                     /locus_tag="VHS97_11100"
     CDS             13262..13852
                     /locus_tag="VHS97_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448793.1"
                     /translation="MSDNFQMLVDAEVTLGQADMVKQRVLAVLHLHDLIVREADMECA
                     LGSLGYRPGPAVHQLYELAENELPFWDQITCGVELRIGRDFNHWAYGTSCEGYSCPLC
                     SHEFEPEDEDLAEVLSEAIGEWVDQSGPALVPCPSCTKQVPVSQWRCTPPLGFGNLSV
                     VFWNWPPLDSPFWRIDILELIRQATLHPLIRTHGHL"
     gene            complement(14155..14547)
                     /locus_tag="VHS97_11105"
     CDS             complement(14155..14547)
                     /locus_tag="VHS97_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX3448794.1"
                     /translation="MNKTKEEIEQIAEQVVDATLKVHRALGPGLLESTYQACLAHELR
                     CRRTEVGCEVALPVRYGGIEIEAGYRIDMLVAECIIIENKSVQAIAPIHEAQVLTYLK
                     LSGHRLGFLVNWNVPLIKDGMKRMVNGL"
BASE COUNT         3046 a         4555 c         4420 g         3072 t
ORIGIN      
        1 atcgccatgc caacttcatc gtcgctcatc ccggcgccaa agcctccgac gtgctcgagt
       61 tgatcgaccg gatccgccag cgcgtctggc agcagttcgg ttacgagttg gaacttcaaa
      121 tccaagtctg gtaagctttt gggatggatg attccaggtg atggaacacc gcgaacctcc
      181 acggatcggg cctggcgatc gcgtgttcga tgcttccctc gaggagcccg ccggccgatt
      241 tcggctgcgc cctttgatcg cgggacgcga cgcccgtcag tttgactccg tcaaggtcgg
      301 cgtggtgttg ttggtggggc tggcagtcgc gatcaccctg gtgtttctcg gatcctgggc
      361 gaggcgcgcc acgctcgact ggctggccgt tcaggcaccg tatcaaatac cgttcaaaca
      421 gatccacctt gtcaatcctc cacccccgtg gttcctcggg ggaaaggatg cgctgctcga
      481 aggcgttcgc aagtcggcgg gggagcccga gacgatatct gtgctccagg tatctcccga
      541 gcaacttgcc acggtgttca agaaatatgc ctgggttgaa ggggtcaagg ttgcctaccc
      601 gcccgggcag attgacgttg agctccgtta tcaccaaccg gtggcatggg tccagttaag
      661 gttggccgac cggatcgtga tcgatgaaaa gggcaatatt ctgccggccg aagacgttga
      721 cgtgtcgcag ctgcgcaaat tgatcatgat cataggggcc ggtttagctg ggccggcgga
      781 ccggcagcct ggcgtggtct ggaaagttcg cgacagtaac ggtctcgagc aaattgatga
      841 gggaattgtg gctgcggcaa agctggctag ttttctcctg gaagagccgc aaaagggcga
      901 cgcccaacga tccaatgcac tgcgcattct taaaatcaac gtaaccgatt cctcaagccg
      961 tggtcttttt gtctacaacg ccgaagatat tacgatcctc tggggtaaag cgccaggaga
     1021 tgagtctccc ggcaagccaa cggccgccga aaaatgggcc atgcttcgcc gctggcaaga
     1081 aacggaaaag gcacgatttt tggcggacgg cgatttctgg gccttttcaa acgatcggct
     1141 tcgctttgtc tgcccctctc ggcacccgac acgtcaccag ccgttcatca aaaagctcta
     1201 tgaagaaccc ggcgatccct ccgatttgca ctccaaaccc cctggatcgg gataattgag
     1261 gttgaggctg tcccgttatg tctgaggagt cgcgcgcggg tgcttcgcgc gccggtgaga
     1321 agatgggtca tgccgggcgg ctgccggcga tgcaccatga tggcaaaccg agggggagga
     1381 tgacacacca tggccagtcg ccttgggccg aactggttga atcgattttc acagctcgcc
     1441 ggaccgaccc acgccggccg gctaggccgc tacgccattt tggtcgatcg aatcaacgca
     1501 ctcgagcccc tcttcgaggt cttgagccct gagcagttac agtctaaagc ccacgacctg
     1561 aggctgagag cccgccaggg tgactcgctc aaaagtcttc tggtcgaagc attcgcgcta
     1621 gtgcgcgaag ccgccaagcg cgcgatcggc caacgccagt tcgatgttca gatcgtcggc
     1681 agtatcgcga ttcacaatcg ttgcatcgcc gagctcgaga ctggtgaagg caagacgttg
     1741 gtggcgacca tgccggcgtt cttgaacgcc ctccccgcca agggggtcca cattgtcacg
     1801 gttaacgact atctggccca ccgcgacgct gattggatgg gtccaatcta tcggttgcta
     1861 ggcctgaccg tcggctgtat ccagaccggc cagagcgacg cggaacgccg tcaggcctac
     1921 gcctgcgaca tcacttacgg aaccgccaag gaactcggtt ttgacttcct gcgcgacgaa
     1981 ctgaaaaagc ttcagctcgg tggagacgct caccgcaaaa ctttcgaaga ggtctacctg
     2041 ggacgcggtg gccactacga ggctcaaatg ccggtgcaac gcacccacgc gtacgccatt
     2101 gtcgacgaag ccgacagcat cctgatcgat gaggcgcgaa ctccccttat catcggtgcc
     2161 aataaccagc cgacccagga agaggctgcc gcgtactacg gcgcggacca gctcgcggcc
     2221 accttggtcc gcatcaagga ctataaatat gatcccctgg agcgtaaggc cgagctcacg
     2281 gccacgggca gacgcaaggt tcaagcggtc gccgcgaacc cggcgtttgt cagcctgaca
     2341 gtcgacgggc tctacgaata tgtggagcgt tcgcttcgcg cccagatcgc gtatctcaaa
     2401 gaccgcgact acgttacggt cgatggcgag gtcgtcatcg tcgacgagtt taccggccgt
     2461 atgatgccgg gacgccagtg gcaggatggg ttgcaccagt ccatccaggc gaaggagcgg
     2521 ttggagatca ccctggaaac catcaccgcg gcacgcgtaa cggttcagga cttcttcaaa
     2581 cgctacaaca agctcgccgg tatgacaggc acggcttcgt ccgacgccgc cgagctccgc
     2641 cggatttaca aggtcggcgt cttcaaggtg ccgaccaacc gccccggccg ccgcgtttgg
     2701 ctgcccgacc gcgtattctc caattcggaa gaacggtttc aggccgtggc cgatcagatc
     2761 gccgaatgga acaagttggg cgtccccgtg cttgtgggca ctcggtccat cgaaaagtca
     2821 gagcggttga gcgcgctttt ggaagcgcaa ggaatcgaac accagatctt gaacgccaaa
     2881 aaccacgaaa tcgaggccca gatcgtagca caggccggcc agaacggccg cgttacggtc
     2941 gcgaccaaca tggctggccg tggaaccgac atcaagctcg gtgagggtgt cgccgagctt
     3001 ggcggactgc atgtcatcgg tacagaacgg cacgaagcac gccggatcga ccgccagctc
     3061 tccggccgct gtgctcgcca gggagacccg ggcctcgccc aatttttcat ctgccttgac
     3121 gacgagatca tcgaggcatt cggcgaaaaa tcggccgccc gaattcgcaa gcgcttggga
     3181 ggtacgggtg agctcaccag cgtccgcatg cgacggtttt tcgtcgccgc ccagcgcaag
     3241 aaagaacgtc agcacttccg cgatcgtaag ttgctcatgc attacgaaaa gcagcgcgcc
     3301 gagatgcgga aaaacatggg cttgaacccc gtgctcggct gatttgaagt ggctggccct
     3361 caacgggcca gccgcttcaa atagcggtca gccacttcac gatcgagctt cttgagcgat
     3421 cccaagtgtt tttcggcgag agcaacgcga ttggctgcgg tcccctgccc ttgcggcggc
     3481 aattccgcca ggtacgcccg gaattttggg aactgggcgt ccgttttcat caggtagtgc
     3541 accaggagcc agctctcgcc gtacgagagc tgctgcgtct tctcgtccca aaacgtcttg
     3601 tcgtcagcaa ccaggtcggg aatcacgatc caactccggg acgttcgcaa acactggagc
     3661 cacggtcggt tgactgtccc gatcgaagtc gccggctttt ttcgttgcca tagctcgaca
     3721 taagtggcca acccttcact gacccaatcc ggcacatccg tctgccgcga gagcagccct
     3781 gtattgaagc agagcaaatg cgttgtctcg tggacaagag ctagcaaatt gacacgttgg
     3841 ggatcagcct gcacgtcgat ctcgtcgctc gtcggtcgaa agtcaaacat gaccaatcga
     3901 ttggtgtcga gatcgtagtg cccgccaacc atcttgcccg ggtcttcgcc gagaaacgct
     3961 ccgtacgact ctttgtcctt gagcgtgatc accgtcaacc gctgggccgg catggccaga
     4021 ttgaaacctt tttgtcggaa atggggcaga aatgccgcag ataatgattc acagatccca
     4081 agtgcggtct tgcgaaaggc ctcaggcgcg tctcccagac caaggaaatg gtcagtatgg
     4141 ctgtctttga acggcaccag accggccttc ttcgccagag cttgaaccgc ggcgatctcg
     4201 tcggcgtcgc tggcatctac cttgctttgc atcgacaggc caacgcgcgg catggccggg
     4261 cccacggcgg cagccaacaa cactcgcttc aaccaggagc gtcgggaaat gagcgagatc
     4321 ggcaagattc ctcgggcgct tgaaagcgat agacttcggt acatgatcga tcgtgctccc
     4381 acggacgcaa cgcaacagag ccaaggggca ctcagcttca ttgattgtaa cgcgcctgac
     4441 gggcgcgcgc acaaccaccg ctccgcccgt gccgaagtgg gggcacgaac agtttctggc
     4501 atcattgtct cttccttcta ggacgatgca catggttttc gcgttgaaaa cccagggcgt
     4561 tgccctgggc tgtcgtgtct gggccctttc agggcaagaa cgcgatggtg ccgtcccagc
     4621 gttttcggcc ccaagggcca gtcacatagc agcccagtgc aacgccctgg gggcccgata
     4681 cacagcaaat accccgccct gcaagggccg aacatcggca attgtccgcg ttcgccagaa
     4741 tcgattgccg caccctgccg aacgtgcttc ccttagattc ctcttgtgaa tgcgggcccg
     4801 acggaataac gtatccgctt ggcgatggac cgcaacctgc ttctgtttcc cagcttgccc
     4861 cacgaagcat cacggaggat gtcaacatgc tgcgggccga tacacacaac ctatcgtttt
     4921 ccacccgcac gcgtctgttg atcaagtacg cgatgtatcc gggcaccaac tggatcagcc
     4981 gcgataaggc gaagattgtg aagatgttct tgcgagggac ccatgaccgg ccggtccgca
     5041 ccctcgattg tggctgcggc aatgcctatt tcgcccacca ggccgtgctc cgtggttcgc
     5101 gctgcaccgg tatcaccatt cacgagtggg agaaacataa ctgtgaagag atgcgcagtt
     5161 tcctcaatgt ttcgcacggg gaccttgagt ttcgagttgc caatctcgaa tccctcgcgg
     5221 ccgacccact cgagcatggt cagtacgacc aggttttatt cctcgacgtc atcgagcaca
     5281 ttctcgaagc caggtcggct ttgcgccaga tccatgccct gactgcccag gatggattcg
     5341 tgtacatcac gacccccaac cgcgactggc agggaaatgc cgaccggatc cgggtgacac
     5401 gcgacgaaga tggctggcac ctgcggaatg gatacacatt tgaacagctg gaaacgctcc
     5461 ttgaggaaac cggtttcgag cccgtcgacc ggctgcgttt cggaactgcc ggctcgacac
     5521 ttgtcacctg gattcaacac aaattgtttc aaaggtggat cgacccgctc acggtcgcct
     5581 tcttcccgtt gctcaagata ctggcagctg ttctgtctcc ttggcgggac acgcatacga
     5641 tctttgtgct ggcgcgaaag aagcgcggtt ctgcttgcag cccggaccgc gccgattgac
     5701 cacatggtgc ccagcgcgta gattgaacga ctcggttgat ggccttatta accttggatt
     5761 ggtccagtcg ccaggtgccg tttgatgagc gacgtccgct attcgcctcc atttttcgtc
     5821 gggatcgacc tgggcgggac caacatcaag agcggcgtgg tggacaacga agggcggtcg
     5881 ttgtcatccg ttagcatcga aacgcacgcg gagaaaggcc cggtcgccgg cgtcgatagc
     5941 ctggtcgccg ccgcgaacaa ggcagtcgag gccagtggga tcaaatggga tcgcatcgcc
     6001 gccgtcggcc tgggctcgcc cgggacaatg gacctcgagg ccggcatgct gctcgacccg
     6061 cccaatcttc cgggctggga caatttcccg atccgccagc ggctcgccga gcgacttcag
     6121 aagccgacgg cactgcagaa tgatgcgaat gccgcgtcgt acggcgagtt ctgggtgggt
     6181 gccgggaaag gcggccgcag cctggtcatg ttcacgttgg ggacaggaat cggctgcgga
     6241 attgtggtcg acggcatgat catcgaaggg cggcacagcc acggcgccga gtgcggtcac
     6301 atcattatcg agatggaaaa cggccgtcag tgttcgtgcg gtgcctatgg acacctggag
     6361 ggttatgcgt cagcgacggc gctggtcaag cgcgcccatg aggccttggg tgccgacacc
     6421 aaacccagcc gtttgaggtc gctcgagcat gatgagctca ccagccgggc catcgcggag
     6481 gcggcggact cgggcgacga attggctcgc cgtttgatgc gcgagacagc ccattacctg
     6541 gcagtcggcg cggtctccct catgcacacg atcgacccgg acatcgtgct cttcggcgga
     6601 ggcatgatcg ccgccggtca accgtttttg gaagccattc gagccgatat tcgcgcgatg
     6661 gcctttcccg tgccggcggc gaagacgcgc gtcgaatacg cccaactcgg cggcgaggcc
     6721 ggattcatcg gtgcagcagg ctgcgccagg ttggcgtttc accggccgga ttaggatcaa
     6781 taacaatatc cttgacctcg agctgcacct cacgatgctc gttccactca ttgatcgacg
     6841 ggctgaacac gatcgaacag cgcgcccccg ccgccagatc tctactcttt tccgccaggt
     6901 tccaagcgat ggccttcagc aggtggttcc cttgtttgag ccggagctgg aggtggtttt
     6961 tcttttcgcc aaccggccgg ggcgcgcccg caacttcgac agcgctggcc aacaagagtg
     7021 gcctgggatt ggagattccg tacggctcga gcttttcaat ctcctcgacg accccaaggc
     7081 tcaagattcc cagcggcact tccgcgtcga tcgacaagac acgctcgagc tgttcggatg
     7141 aaagagaggc cttgcaccgt tcctcaaaac ggcgggcaaa gactggaaag tggtcggcat
     7201 ggagcttcaa gccagccgcg gccgcgtgtc caccgtacgc gagcaatcct tccgagcagt
     7261 ctttgagtgc ttcatacagg tcgaatccgg ggatcgaccg cgccgagcct tgcgatatct
     7321 cagttcccaa ggaaatgatt acgaccggcc gatgaaacga ttccgccagc cggccggcga
     7381 caataccgat cacgcccgca tgccagccgg gacgtcccag cacaatcgcc cggcgatctc
     7441 ccaggccccc ctcggagtcg atgatcgcct tggcttcctc ataaatctgc cgttcgacct
     7501 cctgccgtcg tgcgttgcaa cgatcgagct cgccggcgat ctcgcgcgcc cgcggcaggt
     7561 cggcggttgt gagcatttcg acggccatca tggcacgttc gagccgacca gccgcgttga
     7621 tccgcggcgc caggccaaaa ccgatcgtgc cgctcgtgag ttttcgccgg tcgcgtgcgc
     7681 cactcacttc cagtaacgcc acgagtcccg ggctcgggcc cgtcgaaatc cccgacagac
     7741 cgtgccgtac catcacccgg ttctcgtccg aaagcgggac catatcggca atcgttgcca
     7801 gagcgaccag gcccaaggca cggaccagga attcgcgaag caccggcgag gcgcgcttcc
     7861 cgtcgccaaa gctcttacaa agctgccagg ccagcttgaa cgccaccgcc gcaccgcaaa
     7921 gatcggcctc gaactccggg ccgccgggca agcgcgggtg cacgatcgta tcggccctcg
     7981 gtagcgtcca gccgatcgtg tgatgatcgg ttatgatcag ctcgattccc agctcacgcg
     8041 ccagatcggc ctcgcgcacc gccgagattc cgcaatcgac cgtgacgact agctcggctt
     8101 tgttctccgc cgccagcttg cgcagcgcat cggcattaac cccgtacccc tcctcgacac
     8161 ggtgcgggat gtaatattcg gcatgggcac tgccggccag ccgaaggcat tgccacaaga
     8221 tgctggtacc gcacacgccg tccacgtcat aatcgccgta aatgacgatc ttgcgatcat
     8281 cccggatcgc cctcacgatc cggtcggcag cctcgaccat tccaggtatg agtgccgggt
     8341 cgtgcaggct caccatccgg gcatctaaaa atgctctggc tcggtcgggt tggtcaatgc
     8401 cccggttgag caaaagctga gcgaccaatg gcgagatacc cgcgcgccgg ctcagactgg
     8461 cgatgtcggc cggattgtgg ggtctgatcc gccagcgtgc gttcatcgag cgcgacgtcc
     8521 ccagatggca caacgcgcgg ggcgagtcgt caaaaccatt ttctccggcg cgcgtaaatc
     8581 cagataaaac agggcgataa cgtcatgatc gcgcacgctc cccagaagag caaccccttg
     8641 ggaaactcgg tcttgaacgc cagcgtctca ccgaagaaat tcatgccgaa gaacccgacc
     8701 agaaacgaca tgggcatgaa cattacggtg acgatcgtca gggtcttcat gatgtcattg
     8761 gtgcggttgg acaccaccga caaatacgtc tcaagcgtcc ctgaaatcag gtcgcgcagg
     8821 ctttccgaga tgtcgtgaat tcgtaccacg tgatcataaa gatctcgaaa atacacgcgg
     8881 tgcttgggct ggatcggctt atagggatca cgggccagcc tgttcaagac ttcgcgctgg
     8941 ggcgagagga tcttgtggag ctggatggcc gaccgcttga tccggaagat ctttttcaga
     9001 ttcgtgggtg tagcgttgtc gatgaccgcg ttctggatct catcgacctg ctcatcaaga
     9061 tcctcgatgg cctcgagcga ttgatcgatc gccagctcga ggaaccgaaa gagaagatgg
     9121 tcgggcccgt tccgcaagcg atcgcgagga tcgcgcacaa tgctctcgcg atcccggtcc
     9181 agaatcggca agggagcggt gtggtacgtg accaggtaat tcttgcccag aaagacgtcg
     9241 agctcctgga gctccaaggc ctcggtctcg ggaacgatcc gggccacgtg aaagacgatg
     9301 tagagatagt ctccccagtc gtcaaccttg gggatatgcg attcccgtag cgcgtcctca
     9361 acggcgaggt ggtgaaaatg aaactgtgtg cagagccaat tttcgagttc gtcgccggca
     9421 tgctcgtccg ggccttggat gtccacccag agaattccct tatcatcatt gatcgcttgt
     9481 tcgatctgag catacggcca ttcggcgtgg aaaactcccg cgccgtcgcg atagctcaga
     9541 cggatgacgg actcgttctc ggcaggctct tgggcggccc ggggcagtgg atctgccact
     9601 ggggtgaggc cgctcatcga gcgcctccgg tcttccgtga atccggggcc gctggcaaac
     9661 cgagcgaact ttgatggcgg atcagttctt cgaccacgtc gatcacaagc ggctcggtgg
     9721 gccccgccgc gatctcgcga cggatcggcg actgataggc tccgaccacc ctgattgcgc
     9781 ctggggtatt caggggggcg ttgacaagcc gaatcaggtt gacgccgatc gcgaccttgt
     9841 cggcgacaaa cgaaccgtcc gggccgagct tagccgaaca aaggttgccg acagttccat
     9901 caaccggaat aaattcgatc cagccccccg cgacaggccg gcttccctct cgaaccaccc
     9961 cgctaatccg tgccaccaga aatcgctcag gtcctagctc ttccgagcag ccggccgcgg
    10021 cgacgcttat ccagagaagt acgaagagat cgagtcgcgt tcgcagcctt ggtctatcag
    10081 tcatcgtgtc tggagagacc gctctagcgg tttttgactt tgccggccaa cggcgcagcc
    10141 ggcgctttca actggacttc gggcaccaag gcgaccggaa attccgtccc ctccggcacg
    10201 aggtgccggt ctgcaggagc gtttttctcc aggaaatcct tgaggccggc gacgcgcgcc
    10261 accagttctt tttgcctgtc gggtatttgc ctggagccag ccgcggtgag atcgacggcc
    10321 gccctggcga ccggcttgac aagatcaaat accgattgga catacgccgc tgaatctccc
    10381 gcggcgacat gcaccagccc ggagtctcgg gcgcccggca caccatcaaa cgcctgataa
    10441 actggcccga tcaaaagcgc ggtcagatat ttcgccctat cctgattcac cctgtcctgg
    10501 attttcgtcg catacccgct cgcgatgcga tttccgagct cggcggcaag ttgggcggcg
    10561 gagtggacga ccagccgata attgtatttg ggaaccgacg atgagatctg catgagccca
    10621 agggcgcgtg ccgcctcgga tcgaacttcg aatttggcac tcccatcggc aagcaagctc
    10681 atggccgcgt tggccatgtc ggcctttttt ggtcgattgg gttcgaagcc ctgccgcatc
    10741 gcgctcaagg cttccatggc gcgaagctgc accggccagg ggatcccgtc ttcattgtcg
    10801 aggtagtccg agaccgtctt ggccgctacg atctgatctt gcgctgtcaa gcggcccccg
    10861 ccttcctcca ccacgttgac tataccctcg agtgcccata gcttgaccca tgtcgtctgg
    10921 ttgggatcct tgatttgagc ctcgtaaagc ggcaagaaat cagctgctcc acattctccg
    10981 aggatgatca tcgcctggac tcgagggatc aggtggttct tgagtagcgg agtgagggag
    11041 ttccgcaact cccgatgata gatttgcaga aaaggtagat tcttgatgct cctggccccg
    11101 aacagcggct ctagaagcgc tgtcgtcgct tcctggatgc cgcgcgtcgc cggagcactg
    11161 aggctttggt taccctcgac caacgcctgc acattgcttt tatcggttag cttcgcggcc
    11221 attgcagcga cgacgctctt aatcaagttt tggtcaatgg tggcattagg atcgccagct
    11281 tgggccttga gctggaggat ctctgcgttt gtcaccggtc aggctgtgac cggtttcagt
    11341 ttcgccaggt cgagccaggc ctcggctctt ttgtccctga agatttcaac gggggcgatc
    11401 ttacgggtct tgctcgggtc ggcgtttgcc gccggtgccg gtgcctccac atccggcgcc
    11461 ggggccgctt tgccggtagt cgccttggcg gccggaccac ctgtggcggc tgagcctgcc
    11521 tttggagttt tggacgcgtc tggcgagcct ttcttggagt caggctgcgt ttgtgcccgc
    11581 gccgacatct ggaacaagaa aagcacgagg ctcaaactca gcaccacccc atgccagacc
    11641 cgcgggaatt cccacttcct caacgggaga agcgccttga gcatgtcgcc aaacctcgga
    11701 aaaagcgttt gtaaagatgc gtgcgtcagt agttggacgg aattgtaccg tcaactcatc
    11761 ttacggcgaa ttctacactt ccatcaaatc ggcaatagtt caacgtaacc cgtcaatcac
    11821 ccgctgtcaa gtcgacggcc gccccccagc gaagtaggat cactccgtcg gctgacgcgg
    11881 cgtcggcgag aggcaagtta gacgcgacgt gagccttccc gggagggcga ggtgcgaatc
    11941 gaacttcgat ccagcgctac caacttgact cggaatctgg acattttcgt cgccggggcg
    12001 acaaaaaggt tcccccggtg caccaaccct ttcaatggtt tgacgcatat ctgccagaaa
    12061 tcgttcccaa tcttgtcaga ttggttttcg tcgtcgttga gaccgtcgtc tactcggcgt
    12121 agaatgagat cgaatttcac gttccggtct ttcgcgtcgg cgggcgccct gatcatctgt
    12181 tgttttcaac tcgttggtgt ggatagtcgc gggcaagcct tgatataccg ccgccgcccc
    12241 gagatcgggt gcgagtggca agtcgggctc tagcaccata agttggtttt ttctcaggag
    12301 ttaatttccg catgatcgag cggcgttacg tgttccctgg agtgctcgaa atgaactacc
    12361 aggcgcagcg ccggatgggc gtgaacatct acttggtcga tggcggaacc gaattcgcct
    12421 tgatcgatgt cggattcctc gacgagctgc ctgacgtctt ggaactgatc cggcagatgg
    12481 atttcagcct gtccgcatgc aagatgatcg tcgccacgca cgccgacgtg gatcacaccc
    12541 agggactgac tcgtgcccgc gatgtcctaa agtgcgaggt ggccgcgcac cccaagagcg
    12601 tcgccgctat cgaggaagga gacgagattc tcacctacgc gcgcatcgac gcccaaggca
    12661 tccacatccc catgccgcga tgcaaggttg acaaggtgat cggcgagggt gacgagctgc
    12721 aaatcggcga caagacgctc gaagtctgga gtacgccggg tcacacagcc ggtcaacttg
    12781 cgctgcgaat gggacgactg ctcttttcag gcgacaacat cttccgcgat gggggtgtcg
    12841 gagtgattga tgcccatcat ggctcaaatt tgctcgaatt cgtcgccagc ctcaaacgga
    12901 ttcttgcctc ggatgtcgac tacctcctcc cctctcacgg accgatcttt cgcaaggacg
    12961 acgccctctt gagggcgacg atcgcgcggc tcgaggcgta cacgcacatg gccgattttg
    13021 gcacttgcgc ggtcgattgg ccccttatgg atcaatggga agaagagctg gctcgcggcg
    13081 atttacggtt cgacggacca caccgttgag ccgagttttt cggctgccgc tgctggtatc
    13141 ttaagtactc ctatcagccg tatgggccgg ggattctggc gttgggcccg taaagaatct
    13201 ccgtgatctc aggcgtccct ttctcctgac ggaggcacgg cgtttcgatc gtgtttgcat
    13261 tatgagtgat aacttccaga tgcttgtcga tgcagaggtg acgctcggcc aggctgacat
    13321 ggtaaagcag cgcgtactcg ccgtgcttca cctgcatgac ctgattgtca gggaagccga
    13381 catggaatgc gcactcggta gcctgggata tcgccccgga ccggccgttc atcaactcta
    13441 tgagctggca gagaacgagc ttccattttg ggaccaaatc acttgcggag tcgaacttcg
    13501 gatcgggcgt gatttcaatc attgggcgta tggaacgtcg tgcgagggct attcatgccc
    13561 tctttgcagc cacgaattcg agcccgagga tgaagatttg gctgaggtgc tttctgaagc
    13621 catcggcgaa tgggtcgacc aatcgggacc ggcgctcgtg ccgtgcccat cctgcacgaa
    13681 gcaagtgccc gtctcgcaat ggcgctgcac gccgccattg ggctttggca acctgtcagt
    13741 tgtcttctgg aactggccgc cgctcgactc gcctttctgg cgaatcgaca tcctcgagct
    13801 tatccgccaa gcaacccttc accccctgat ccgtacccac ggccacctct aaaaggggcc
    13861 aggagcggtc cggatccgca accgttcacg ccctggggcc ggcgggttac ccggctcaga
    13921 gccacggtcc gcgactgggt cgcacgactc agacggaact ccaccgaatg gttcaaactg
    13981 gaatcgtacg acgcagtcga attgacgata gaggttcacg gttacagcgc aagatcgttg
    14041 aacatggaat gaatctgggg cacaaagacg gcagcgccgt cggtggccta acagcttgaa
    14101 gagagagatt caccgcgacg acgcgaagag cgcgaagaaa ggcgatgaac gtgttcataa
    14161 gccattgacc atccgcttca tgccgtcctt gatcagagga acgttccagt tcacaaggaa
    14221 gccaagtcgg tgtcccgaga gcttcaggta cgtcagcacc tgtgcttcgt gaattggggc
    14281 tatcgcctga accgacttgt tctcgataat gatgcactca gccacgagca tgtcgattcg
    14341 ataacccgcc tcgatctcga tcccgccgta ccttactggc aaggcaactt cgcagccgac
    14401 ttccgtacgc cggcaacgaa gttcatgggc caggcatgcc tgatacgtcg attccagcag
    14461 gccgggcccc aatgcgcggt gtaccttgag cgtggcgtcc accacctgct cggcgatctg
    14521 ctcgatctct tcctttgtct tattcattgc catttcccct tcacgcgtgt cttattcatt
    14581 gccatttccc ttcgcgccct tcgcgtcttc gcggtgagaa agtgcatcga tttgatcccg
    14641 tgagcgcgag ctcgctcttg atcccatcgc acttccgtag acaacgccca cggcgaggta
    14701 gcctttcgtg gggtgagaga ggtatcactt gggcccaagg ccgaaataga ctcaatttgg
    14761 gcagaaggac cgcagcgccg tcggtggtct gatcggccgc tgtgaggtcc gcgcgcacgt
    14821 tcacgctgct tgaagagaca gattcaccgc gacgacgcga ggagcgcgaa gaaaggcgat
    14881 gaacctgctc ataagccatt gaccatccgc ttcatgccgt tgctgatctg agggccgtcc
    14941 cagttcacga ggaagccaag tcgatgtccc ggcaacgaag ttggagtcag aatatattga
    15001 tggagtttag acgatcaacg aatcatccac gaagatcgtg ttgacgaagg tgccgccccg
    15061 gatgctgtag cctttggctt tcatcaattc gag
//
LOCUS       DATAQL010000633         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3729406, whole genome shotgun sequence.
ACCESSION   DATAQL010000633 DATAQL010000000
VERSION     DATAQL010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3729406"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VHS97_11110"
     CDS             complement(<1..1338)
                     /locus_tag="VHS97_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX3448795.1"
                     /translation="MADPETPDWVRDAVFYQIFPDRFARSLAVPKPRHLDEWGAQPTY
                     HGYQGGDLIGVVEHLDYLVDLGVNALYFTPIFQSASNHRYHTHDYEKVDPMLGGTAAL
                     RRLIDEAHERNIRVVLDGVFNHASRGFFQFHDIMENGPNSAYLDWFHIKEFPLNAYDS
                     GKAPNYRAWWGLPALPKFNVGCSEVREFLWGIAKTWVEFGADGWRLDVPNEIDDDAFW
                     REFRVRVRAANPDAYIVGEVWTDSQRWLNGDMWDAVMNYQFTKSCIAYFIGDSLNEDA
                     LKKTSLYPPGPASAEAFRRNIERLLGLYHPNVSSVMLNLLGSHDMARFLTLARGDTRA
                     LKLATLFQMIYPGAPSIYYGDEIGMTGGHDPANRGAFPWHNHELWDRDLLHYFQRLIA
                     LRRERPALRRGTFQFLWAADGVSAVARQLDGETVIALFNTSQETRRLDLPVANL"
     gene            complement(1446..>3261)
                     /locus_tag="VHS97_11115"
     CDS             complement(1446..>3261)
                     /locus_tag="VHS97_11115"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX3448796.1"
                     /translation="LGRVQQNNAEERERVVRFLMDVGWLAEAKQELDRLVKDFPDPNL
                     KERAASARQFIIQAEAIERRGEIDVSKRAQQYDRAAKLLKSFNDNAIPTQLLVEVREI
                     ERRDQQQRAADLGVAAELRKLADELPSADRKFWKEPVAEVLKALGEAPDAVRDRLAAW
                     RKARAQAGSKPQTQFALAMSGSVAGIDMAVRDLKDAQTLWKARDIVASYLTGPPGPRS
                     ELLTQLDGLDWQSAIGSADVHRRLEVLTRLIQLMPPPRHDGQTEPEKTTTNRVMDDEN
                     NEPTVYAVRLPPEYHALRSYPAIVVLHGGEGPAKAIEPWAAQAARRGYILIAPEYTVP
                     GQPPGYHYTANEHAAVELALRDARQRYAIDSDRVFAAGQLTGGDMAWDYSLAHPDLFA
                     GVVVISGLPFKFVPRYVSHHERLPLFFVIGEKTPAANNFIYEKYVKPLILKTWDVTYV
                     EYYRRGREELPEEIVPAFEWMDRHRRDPYPKSFKVLTARDSDNRFYGTVVREFTPGRT
                     TAPEAALELGQNLNPASIEMKSSNLSNLIKLEVNGIKRLDVWLSPKLIDFKRRMEIRI
                     RDKPYYRGQVKLECEPMLEDLRVRGDRQQLYWHRISAG"
BASE COUNT          611 a          977 c         1036 g          637 t
ORIGIN      
        1 cagatttgcc accggcaagt cgagtcggcg ggtctcttgg ctggtgttga agagcgcgat
       61 aacggtttca ccatcgagct gacgagccac ggcggaaacg ccgtcggccg cccagaggaa
      121 ctgaaacgtc ccccgccgta gcgccgggcg ctcgcgtcgc agggcgatca gccgttgaaa
      181 gtagtgcaac aagtcccggt cccagagttc gtgattgtgc caggggaatg caccgcggtt
      241 agccgggtcg tggccgccag tcatgccgat ctcatcaccg taatagatcg atggcgcgcc
      301 gggataaatc atctgaaaaa gggtcgccag cttaagagcc ctggtgtcgc cgcgcgccaa
      361 agtgaggaac cgcgccatgt catggctccc gagtaaatta agcattacgc tcgacacatt
      421 gggatgatac agacccagca gtcgctcgat attgcggcga aacgcttcgg ctgacgccgg
      481 cccgggtgga tagagactgg tcttcttgag ggcgtcttcg ttcaacgaat cgccgataaa
      541 gtaggcgata catgattttg tgaactgata gttcatcacc gcgtcccaca tgtccccatt
      601 gagccagcgc tgcgagtcgg tccagacctc gccgacaatg tatgcgtcgg gattggcggc
      661 gcggacgcgc acgcggaact cccgccagaa ggcgtcgtcg tcgatctcgt tggggacgtc
      721 aagccgccag ccgtcggcgc caaactcgac ccaggttttg gcgattcccc agaggaactc
      781 gcgtacttca gagcacccca cgttgaactt aggcaaagcg ggcagacccc accaggctcg
      841 atagttgggt gctttgccgc tgtcgtaggc gttgagcggg aactctttga tgtggaacca
      901 gtcgagatag gccgagttcg ggccgttttc catgatgtcg tggaactgga aaaagccgcg
      961 gctggcgtgg ttgaatactc cgtcgagaac aacgcggatg ttgcgctcgt gggcttcgtc
     1021 gatcaggcgg cggagggcag cggtcccccc gagcatcggg tcgacctttt cgtaatcgtg
     1081 ggtgtgatag cggtggttcg atgcggactg gaaaatcggc gtgaaataga gggcattcac
     1141 gccgaggtcg acgagataat cgaggtgctc gacaacgccg atcagatctc ccccttgata
     1201 cccgtgatag gttggctgcg ccccccattc gtcgaggtgg cgcggcttgg gaaccgccag
     1261 ggaccgcgcg aagcggtcgg gaaagatctg gtagaaaact gcgtcgcgaa cccagtcagg
     1321 cgtctcggga tcagccaccg gcggctccgt gattcgggtc caggctggat tttctgtgcc
     1381 ccattgcgca tggggttgcg gtgaagtcgg cagggcatcg accgcgcgag tctcatgcgc
     1441 gagactcaac ccgccgagat ccgatgccag tagagctgct ggcgatcgcc gcgaacgcgc
     1501 aggtcttcga gcatcggctc gcattcgagc ttcacctgcc ctctgtagta cggcttgtcc
     1561 ctgatgcgga tctccatcct ccgcttgaag tcgatgagct tgggggaaag ccagacgtcg
     1621 agccgcttga tgccattgac ctctaacttg atcaggttcg agaggttact cgacttcatc
     1681 tcgatcgagg cgggattcag gttttggccg agctcgagcg cagcctctgg cgcggtggtg
     1741 cggccggggg taaactcgcg aacaactgtg ccgtaaaagc ggttgtcgga gtctcgcgcc
     1801 gttaacacct tgaacgactt cgggtacggg tcgcggcgat ggcggtccat ccactcgaat
     1861 gcggggacga tctcctccgg gagctcttcg cggccgcggc ggtagtattc gacgtaggtg
     1921 acatcccagg tcttcaaaat cagcggcttg acgtattttt cgtagatgaa attgttcgcc
     1981 gccggagtct tttcgccgat cacgaaaaac agcggcagcc gctcgtggtg cgaaacgtaa
     2041 cgggggacaa acttgaaggg gagccccgag atcacgacga cgccggcgaa caggtcggga
     2101 tgggccaggg aataatccca tgccatgtca cccccggtga gctgaccggc ggcaaagacg
     2161 cggtcgctgt cgatagcgta ccgctggcgg gcatcgcgca gcgccagctc gaccgcggca
     2221 tgctcattgg ccgtgtagtg ataaccgggc ggctggcccg gcaccgtgta ctccggcgcg
     2281 atcaagatat acccccgacg agccgcctgc gcggcccagg gctcgatcgc cttggccggg
     2341 ccctcaccac catgcaaaac gacgatcgcc gggtaactcc gaagcgcatg atactcgggt
     2401 ggcagccgca cggcatacac ggtcggctcg ttgttttcgt catccatcac gcggttggtc
     2461 gtggtcttct ccggctccgt ctggccatca tgccgcggtg gcggcatgag ctgtatgagt
     2521 cgtgtgagca cttcgagccg gcggtgcacg tcggcggagc caatggcgga ctgccaatcg
     2581 aggccatcaa gttgggtgag cagctcgctg cgcgggccgg gcgggcccgt gaggtaactg
     2641 gctacaatat cgcgcgcttt ccagagcgtc tgagcgtcct tgagatcccg cacggccata
     2701 tcgataccgg cgaccgagcc cgacatcgcc agggcaaatt gggtttgcgg tttcgacccc
     2761 gcctgagccc tggctttgcg ccaggcggcc aggcggtcgc ggacggcatc gggtgcttca
     2821 cccagggcct tcaagacctc cgcaacaggc tctttccaga acttgcggtc ggccgacggc
     2881 aattcgtccg cgagcttgcg cagctcggcc gccaccccca agtcggcagc ccgctgctgc
     2941 tgatcacgcc gctcaatctc acgaacctca accaaaagct gcgtcgggat cgcgttgtcg
     3001 ttaaatgact tgagcagttt cgccgcgcga tcatactgct gtgccctctt gctcacgtcg
     3061 atctcgccgc gacgctcgat cgcctcggct tgaatgatga attgacgcgc actggcggct
     3121 cgctctttca agttggggtc gggaaagtct ttcaccaggc ggtcgagttc ttgtttggcc
     3181 tcggcaagcc agccgacatc catcaaaaac cggaccacgc gttcgcgctc ctcggcgtta
     3241 ttctgctgga cccggcccag g
//
LOCUS       DATAQL010000634         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4492587, whole genome shotgun sequence.
ACCESSION   DATAQL010000634 DATAQL010000000
VERSION     DATAQL010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4492587"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VHS97_11120"
     CDS             complement(<1..1001)
                     /locus_tag="VHS97_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3448797.1"
                     /translation="MVEAVEKVQRSVVSISSEKLASSHSRWPFSPEENQHRLVNGMGS
                     GVLVDARGYILTNHHVVDKVQGIIVQLFDGTSYPARVLQFDAAMDLAVIKVDVDHLLS
                     AVQVGTSSDLMVGESVITIGNAFGYENTVSVGIISALHRDVTLSDEQVYRNLIQTDAC
                     INPGNSGGPLINVDGELIGINVAVRAGAQGIGFALPIDEVKRVATDMLSTRRLASTWH
                     GLVVNEQRRGAKRGVVVSEVQQGSSAEASGFRPGDELIKVGDLMIASPLDVERGMLDV
                     RPGRPATVMIRRGNSETAFSLDVKPLSGTTAGTPDDLVWRLLGMKTLPVASEYVSTVS
                     "
     gene            1607..1972
                     /locus_tag="VHS97_11125"
     CDS             1607..1972
                     /locus_tag="VHS97_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448798.1"
                     /translation="MGRQLEIFDGIDGSMDEKSRRRLMLEQSLAEDPTDSFLRYGLAV
                     QCLREGDTTEGRERLMALIADHPDDQVAAYQQLGQSYVDTGATTEAVAVLRTGIAKAQ
                     AAGDWHAVAEMEQLVASLA"
     gene            2207..>3289
                     /locus_tag="VHS97_11130"
     CDS             2207..>3289
                     /locus_tag="VHS97_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3448799.1"
                     /translation="MSVTTEAPGESPAPSQKAARRDAPSVEGSPPAAASTFTFPNRIQ
                     FGAGARRLLAVEVARLGIMQPLVVTDPGLIDSGVVAAVVGPLAKPAIVYSDIQSNPTE
                     ADVLAGLDRYRNESCDGLIAIGGGSAIDAAKAIRLLVTHPGRLAEYDLTRGGQDRIKA
                     SMPPMVAIPTTAGTGSEAGRGTLIQLRETGRKTIALSNYLLPSTAVCDPELTFSLSSV
                     LTAGTGMDAFTHCVESYLSTTFHPICDGIAVEGLRYISKGLESAVRDGSNAEARNAMM
                     MGALLGGISFHKGLGVVHSLSHALGSEGRVHHGTINAILLPHALRFNRDAAGPRMADL
                     AGRLGLGRTGDAAGHLVTLTELLLARM"
BASE COUNT          621 a          986 c         1012 g          670 t
ORIGIN      
        1 gacacggttg agacgtattc gctcgcgacc ggcagggttt tcatacccag gagccgccag
       61 accagatcat cgggcgtgcc cgccgttgtg cccgacagcg gtttgacgtc gagagaaaat
      121 gccgtctcgc tgttcccccg acggatcatc acggtggcag ggcggccagg acggacgtcg
      181 agcataccgc gctcgacgtc aaggggactc gctatcatga ggtcgccgac cttgatcagc
      241 tcgtcgcccg gcctgaatcc tgaggcttcg gctgaacttc cctgctggac ctcgctcacg
      301 actacgcccc gtttcgcccc gcgccgttgc tcgttgacga ccagcccatg ccaggtcgac
      361 gccaggcgac gagtggagag catgtcggtg gcgacgcgtt tgacctcgtc gatcgggagc
      421 gcaaagccga tcccttgcgc ccccgcgcgg acggccacat tgatgccgat cagttcgccg
      481 tcgacgttga tcagtggtcc accggaattt ccaggattta tgcaagcgtc cgtttggatc
      541 aggttacggt agacctgttc gtcggaaagc gtcacgtcgc ggtggagtgc gctgatgatc
      601 cccaccgaaa ccgtgttctc gtatccgaat gcgttgccga tcgtgattac cgactctccg
      661 accatcaggt cactcgaggt gccaacctgc accgccgaca gcagatggtc gacgtcgacc
      721 ttgatcaccg ccagatccat ggcggcatcg aactggagta cacgcgccgg atacgacgtc
      781 ccgtcgaaca gttggacgat gattccttga accttgtcga ccacatgatg gttggtcaag
      841 atatagccgc gagcgtcgac caaaacgccg ctccccatgc cgttgaccag ccggtgctga
      901 ttctcttctg gagaaaacgg ccatcggctg tgtgaggatg ccagcttttc actcgaaatg
      961 ctcaccacgc tgcgctgaac cttttcgacc gcctcgacca cggccgtccg tctcgactcg
     1021 gatccagctc gaacactcgc tgacagcggc cccgtagcag ccaccacgaa acaaagactg
     1081 agcatgtatc tacggatctg tctctcttcc tgactgatca agggcgttgg cacggcggtc
     1141 gtcccctcag cgatagacag aggctgcact caagtttgcc cgatccggtc ggccgggctc
     1201 ccgatacatc catcccccac tgcggctctc ggcttggttt tgaatcggtt accctgtttc
     1261 ccagcttgag cttagtcggt ccgatgttcc ctatgttccc ctggcttggt aattatccat
     1321 atttcccttc tgaatttccc aaactacgca ttttccgacg gaattatttt cgcgcgattc
     1381 gttttgcgcg accgctatga tcgcgaggcg cgcgtcgtct gtttccggga aaatgaatcg
     1441 ggcaaactgc gggctttggg gggcgcgtcg cgagcaagga tgcgttgaca gcggcgacaa
     1501 aggtccgtta ggttgaaacg agacggtaga atggaatagg cgcccctgaa ccggacccgc
     1561 gaatcgagat cgtcccggag tcgcccagcg gggcggatga ctttttgtgg gacgacaact
     1621 tgagatcttc gatggaatcg atggctcaat ggacgagaaa tcacgtcgac ggttgatgct
     1681 ggaacagagt ttggccgagg atccgaccga ttcgttccta cgatacggcc tggccgttca
     1741 gtgcctgcgt gaaggggata ccacagaagg tcgcgagcgg ctgatggctt tgatcgccga
     1801 tcaccccgac gatcaggtgg cggcctatca acagctcggc caatcctacg tggacaccgg
     1861 cgcgacgacg gaggccgtgg cggttctgcg caccggcatc gcgaaggcac aggcggccgg
     1921 agattggcac gcagtggctg agatggaaca actcgttgcg tcgctggcgt gaattcgact
     1981 gtcgtgggca acgacggcgg caaactcggc ggacggcgag aagctgcgcc ttatggttgt
     2041 caccactcat acaattctgt tgtaggcaat attagccagg ggctggcgac catttaggca
     2101 agctggcgat cgtatgagca ctcagggcga gcgacctgaa ggctcggcct ggcgcgagaa
     2161 agtgtctaac cgccgaggag cgtgggcctt gtcaacgacg ccgtcgatga gcgtaaccac
     2221 cgaagcgccg ggcgagtcac ccgcgccttc ccaaaaggcg gctcggcgcg acgccccgtc
     2281 ggtcgagggc tcgcctccag ccgcagcctc gacgttcact tttcccaacc ggatccagtt
     2341 tggcgccggc gcgcgtagac tgctcgccgt agaagttgcc cggctcggaa tcatgcagcc
     2401 gttggtggtc acggacccgg gcttgatcga cagcggtgta gtcgccgctg tcgtcggccc
     2461 tctcgccaag ccggcgattg tctatagtga catccaatct aacccgaccg aggccgacgt
     2521 cttggctggg ctcgatcgtt atcgcaacga gagttgtgat ggtctgattg cgatcggggg
     2581 cgggagcgcg attgatgcgg ccaaggcaat ccggctactg gtcacacatc ctggtcgact
     2641 cgctgagtat gacttgactc ggggaggtca agacaggatc aaagcttcga tgcctccgat
     2701 ggtggccatt cccaccacgg ccggcaccgg gagcgaggct gggcgaggaa ccttgattca
     2761 gctccgagag accgggcgca agaccattgc actctccaac tatctactgc cgagcaccgc
     2821 ggtttgcgac cccgagctga cattcagcct ctcgtccgtc ttgaccgcgg gaaccggtat
     2881 ggacgcgttt actcattgcg tggaaagcta tctgtcgacg acctttcatc ccatttgcga
     2941 cgggatcgcc gtggagggtc tcagatacat ttccaagggc ctggagtcag ctgtccggga
     3001 tggctcgaac gcggaggcga ggaatgctat gatgatgggt gccttgctgg gcgggatcag
     3061 ctttcacaag gggctagggg tggtccactc gctgtcgcac gcgctgggat ccgaaggccg
     3121 ggtacaccac ggtactatta acgcgattct ccttcctcat gcgcttcggt tcaatcgcga
     3181 tgccgccgga ccgcggatgg ccgacctcgc ggggcggttg ggcttgggtc gcaccgggga
     3241 tgcggcgggg cacttagtta cgctcactga gctgttgctg gcacgcatg
//
LOCUS       DATAQL010000635         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2661249, whole genome shotgun sequence.
ACCESSION   DATAQL010000635 DATAQL010000000
VERSION     DATAQL010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661249"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VHS97_11135"
     CDS             <1..291
                     /locus_tag="VHS97_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3448800.1"
                     /translation="LIGMATLKLRQTQDDIRQKTLKAYRGLSESHRALSLAHGLVGLR
                     KEAEAKAQAAAQTNPSGLLDSSKARESAEVDLVKADLAYRQAHVELLKLIGQ"
     gene            complement(488..1045)
                     /locus_tag="VHS97_11140"
     CDS             complement(488..1045)
                     /locus_tag="VHS97_11140"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HEX3448801.1"
                     /translation="MDNLECCGAQPVSPDVREQLLEPFISGACLTLTELAQTELAVRS
                     VYRMALPRTLGDISAVLGLTAESGEVLVLSFPAQTAAALAGRVLAEVTRAPEDDLVRD
                     CMGELANVIAGQAKTLLAETPYQLLLATPRVLSGPGLEVGSRPDTESLVVVFGSDAGD
                     FALQVCLKWRGLYEAPSAQDGELRQ"
     gene            complement(1090..1905)
                     /locus_tag="VHS97_11145"
     CDS             complement(1090..1905)
                     /locus_tag="VHS97_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX3448802.1"
                     /translation="MTDQDYDFIRKLLRDRSAITLEDGKQYLVESRLAPIVCELKLGS
                     IGDLVGLLRAPFSNGLQQRVVEAMVTTETTFFRDLHPFEALRKSLIPELVRKRQNERS
                     LNIWCAACSTGQEPYSVALLLRENFPALASWKINILATDLSRDVLARAREGRYSQIEV
                     NRGLPAALLVKHFRQHGTSWQLHDHLRDMVVFQELNLVSAWPPLPRMDLVFLRNVMIY
                     FDVEAKKVILGRMAKVLQSDGYLLLGGAETTFNLNDSYRRLNEFKTGFYQLIG"
     gene            complement(1990..2136)
                     /locus_tag="VHS97_11150"
     CDS             complement(1990..2136)
                     /locus_tag="VHS97_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX3448803.1"
                     /translation="MLAQDKASSVVWGMPGYVAQARLADRVLPLSMIGGEIVQRVRDQ
                     KRSA"
BASE COUNT          416 a          701 c          666 g          357 t
ORIGIN      
        1 ttaatcggca tggcgactct gaagttgcga cagactcaag atgacatccg ccagaagacg
       61 ttaaaggcat accggggcct ctccgagagc catcgggcct tatcactggc ccacgggctg
      121 gtcggcttgc gcaaagaggc cgaagcaaag gcccaggcgg ctgcccagac caacccgtcc
      181 ggcctgctcg actcatccaa ggcgcgcgag tccgccgagg tcgatcttgt gaaggcagac
      241 ctcgcctacc gccaggccca cgtagagctc ttgaagctga tcggccagtg atcttcaccg
      301 cggcaccctg cgaatgcggg tcgaactgtc ccttcgccct tcgatccccg agccccatac
      361 gatcgcatta gatcgccgcg gatattcgcc gcctcgttcc tcgctgcgcc accacttgac
      421 ttgaagaggc acgaaacgac gaacggttgc cgccctttga aggactgcaa gtcattgcgg
      481 cccgccgtca ctgtcgaagc tccccgtcct gcgctgacgg cgcttcatac agcccgcgcc
      541 acttcagaca aacctggagc gcgaaatctc ctgcgtcgct gccgaagacc acgaccaggc
      601 tctcggtgtc cggccgcgac ccaacctcga gtccggggcc cgacaggact ctgggagtgg
      661 cgagaagcaa ctggtatggc gtctcggcca ggagcgtctt ggcctggccg gcgataacgt
      721 tggcgagctc ccccatgcaa tcgcggacga ggtcgtcttc cggcgcccgg gtgacttcgg
      781 caaggacccg tccggcgagc gcggccgccg tttgtgccgg gaagctgagg acaagcacct
      841 cgccgctttc ggccgtcagc ccgagcaccg cggagatgtc gccgagcgtg cgggggagtg
      901 ccatacggta gaccgaccgg acggccagct cggtctgcgc cagctcggtg agcgtcaggc
      961 acgcgcccga gatgaacggc tccagaagct gttcgcggac gtcgggcgag accggctgcg
     1021 caccgcagca ttccaggttg tccaagtcga cctcccgccc cggccggggc acgaaccgcc
     1081 acggagcggc taaccgatca gttgatagaa cccggtcttg aactcattga ggcgccggta
     1141 ggagtcgtta aggttgaacg tggtctccgc cccaccgagg agcaggtagc cgtccgattg
     1201 cagcaccttg gccattcggc ccaggatgac tttcttcgcc tccacgtcga aatagatcat
     1261 gacgttccgc aaaaacacca ggtccatgcg cggcaagggg ggccaggcgc taaccaggtt
     1321 gagctcctgg aaaacgacca tgtcacgcaa gtggtcgtgg agctgccaag aggtgccatg
     1381 ttgccggaag tgcttcacca agagcgcggc gggcagaccg cggttgacct cgatctggct
     1441 gtaccgcccc tcgcgcgcgc gagccaggac gtcccgcgag aggtcggtcg ccaggatatt
     1501 gatcttccag gacgccaggg ccgggaaatt ctcccgcaac aagagggcaa cgctgtacgg
     1561 ctcctggccc gtggagcagg ccgcacacca gatgttgagg ctacgctcgt tctggcgttt
     1621 gcggacgagc tccgggatca gggacttgcg tagcgcctcg aacgggtgta ggtcgcggaa
     1681 gaatgtcgtc tcggtggtca ccatggcctc gacgacccgc tgctgcagtc cgttggaaaa
     1741 cggcgcgcgc agcaggccga ccaggtctcc gatcgagccc agctttagct cacatacgat
     1801 tggcgccagc cgggactcga caaggtattg cttgccgtcc tccagggtga tcgcgctgcg
     1861 gtcacggaga agcttacgga tgaagtcgta atcctggtcc gtcatgaagt cgaaccgttc
     1921 ccaagaagga aggacgggca aacaggcagc aagcagagac cccggactgt ctgatcctca
     1981 cacaccttat caagctgacc gcttctgatc cctgacgcgc tggacgatct cacctccgat
     2041 catcgacagg gggagcaccc ggtcagcgag ccgcgcctgg gcgacgtagc ccggcatccc
     2101 ccagaccacc gaactggcct tgtcctgagc cagaatttgc
//
LOCUS       DATAQL010000636         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_217722, whole genome shotgun sequence.
ACCESSION   DATAQL010000636 DATAQL010000000
VERSION     DATAQL010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217722"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..691
                     /locus_tag="VHS97_11155"
     CDS             335..691
                     /locus_tag="VHS97_11155"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3448804.1"
                     /translation="MAAPALVTVFSALADETRLDIVTRLAAGDATLSQLAEHYDMSIQ
                     AVSKHLSVLEDAGVVSRRREAQRRPVQLETKVFDLMSKWIERYRHNAEARYQRLDQLL
                     ANLDDQAPNNGQGTTT"
     gene            688..1173
                     /locus_tag="VHS97_11160"
     CDS             688..1173
                     /locus_tag="VHS97_11160"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3448805.1"
                     /translation="MTAATNREAEIAIDNKTPSITIVREFDAPARAVFRAHAEPDLFV
                     QWVGPDSLDASRVDRWDCRTGGEWRYVSTRDGEEHWFRGCFHEVRPNELIVQTFTYEP
                     WPDNVSLGRYVFHDLAGSRCLLTVTSFADSFEARDAFVASGMEVGVQEGFRKLDRLLA
                     R"
     gene            1518..2138
                     /locus_tag="VHS97_11165"
     CDS             1518..2138
                     /locus_tag="VHS97_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX3448806.1"
                     /translation="MRKLILHMIISTDGFISTGNRDVNPAAHWSEEIQDHYTDLFDRA
                     GGVVFGRNLYEQYVGHYSKVAAGETLPETDLPRDEAHNEDLVARHLSWTKRLMDMDKF
                     VVSNSLPPITPGISVISGDIAPQILQLKQQNGGDLLLMCGPSLFAALTASKLIDEYMF
                     YVYANALGHGDHLYRDIAAPITMTPGRTVSFADGMELREYKPDYTV"
     gene            complement(2425..>2660)
                     /locus_tag="VHS97_11170"
     CDS             complement(2425..>2660)
                     /locus_tag="VHS97_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448807.1"
                     /translation="SNPTRGREGPLRRLDVRGGAERACPCVRPLPGRFPGPGLPGKGG
                     CGDGQGGGPGERDACRASGAGAGFSLGHAVARA"
BASE COUNT          523 a          893 c          784 g          460 t
ORIGIN      
        1 ccgcgatggc ctgttgtgac ggagcacgct acctggccaa cggagcaggc tatctgacca
       61 ccgggggtga atactgcgca ttctggtcgt ggctgtccag gctcagtcaa acctccaagc
      121 aaccacgtct tggtctctgg ccgctttggc cgggccgatg actgcccgcc gcttccaacc
      181 cgctctagca gaccacctct gatccgaagc acgctggcgg caggtcgggg ccgggtgcag
      241 taccggcggc atgaccgacg agttcaacct tgcggttgac aacagaatga aggcaactta
      301 cgctcttctt caaccaaaca gttgaggagg atgagtggct gcgccagccc tcgtgacggt
      361 gttttcagcg ttggccgacg agacgagact cgacatcgtc acccgcctcg ccgccggtga
      421 cgcgacgctg agccagctgg ccgagcacta cgacatgtct atccaggcgg tgtccaagca
      481 cctgtccgtg ctcgaagacg cgggagtcgt gtcccgccgc cgcgaggcac agcgtcgccc
      541 ggtccagttg gaaacgaagg tgttcgacct tatgagcaag tggatcgagc gataccgcca
      601 caacgccgag gcccgttacc agcgcctcga ccagctgctc gccaatctcg acgaccaggc
      661 ccccaataac ggccagggaa caacgacatg acggctgcga ccaaccgaga ggccgagatc
      721 gccatcgata acaagacgcc gtcgatcacg atagtccggg agttcgacgc cccggccagg
      781 gccgtcttcc gagcccacgc cgagccagat ctcttcgtgc agtgggttgg ccccgacagc
      841 cttgatgcca gccgcgttga tcgctgggat tgccgcaccg gcggtgagtg gcgctacgtc
      901 tcgactcgcg acggcgaaga gcactggttc cggggctgtt tccacgaagt ccggcccaac
      961 gagctcatcg tgcagacctt cacctacgag ccctggcccg acaacgtgtc gctcggacgc
     1021 tacgtcttcc acgatctggc aggcagccgc tgcctgctga ctgttacctc gttcgccgac
     1081 agcttcgagg ctcgcgacgc gttcgttgcc agtggcatgg aggtcggtgt ccaggaaggc
     1141 ttccgcaagc tcgacaggct cctggcccgc tgatggcaac agctgcggtc gatgacccga
     1201 ggaggacatc gcgggataac cgattgtcga cttgatttta tgcgcgccca atccacttat
     1261 cagatgcggc gaggcgatgt ccttgccggc ctcgccggcg gcaccaattc acgaaccagc
     1321 gatcgtgtcg tctcgtcgtc gcagacgtgg cgacgacgat gaatgaaagc ggccgaaaga
     1381 cgccgacgcc caggcagcgc ggaccgccga gaccgggcac gccagctgag gtcttcaagc
     1441 acgctacttg caagcccgct atccgtgtct cgatgtataa gcaggccaac agtcagtcac
     1501 aactacctgg aggacaaatg cgaaaactca tcctgcacat gatcatctcg accgacgggt
     1561 tcatttcgac aggaaatcgc gacgtgaacc ccgcggcgca ctggtccgaa gagatacagg
     1621 accactacac ggacctcttc gatcgggccg gcggtgtcgt cttcggccgc aacctctacg
     1681 agcagtacgt ggggcattat tcgaaggtcg ctgccggaga gactctgccc gaaacggact
     1741 tgcctcgcga cgaagcgcat aacgaagact tggtcgcccg gcatctctca tggaccaagc
     1801 gcctgatgga catggacaaa ttcgtcgtct cgaactcgct ccccccaatc acgcccggga
     1861 tcagcgtcat ctcgggtgac attgcacccc agatcttgca gctcaagcag cagaacggag
     1921 gggacttgct cctgatgtgc ggaccaagcc tgttcgccgc actgaccgcc agcaagctca
     1981 ttgatgagta catgttctac gtctacgcga atgcccttgg tcacggcgat catctgtaca
     2041 gggacatcgc cgcgcccatt acaatgaccc ccggacgcac cgtttcgttc gcggatggca
     2101 tggagcttcg cgagtacaag ccggactaca cggtatgacg accataaagc agtgacacgt
     2161 ccttttcgga gatccgtccc cgggctccac ttatgctcgg tctgtgactg gggtggaagt
     2221 ctctggaatg acgtcggctg cgaagttgcg gcggctcgag ggcaggaccg ctggcttagc
     2281 ggccgccacg ctctccttca cctggaaaac gtcctcaccg aactgcacgc cgtcacgatc
     2341 cgcgaagggc tcgccttccc cgagccctgg cgaccgcatg gccagatacc ccgcccatct
     2401 gtaccagatg gccaagtaca gtgctcatgc tcgcgcgact gcgtgtccca aggagaaccc
     2461 cgcgcccgct ccgctcgccc ggcaagcgtc acgctctccc ggaccgccac cctggccatc
     2521 accgcacccg cccttccccg gcagccccgg cccgggaaac cggccgggca gcgggcggac
     2581 gcagggacat gcacgctcag ctccgccgcg aacgtcaagc cggcgcaacg ggccttcgcg
     2641 gccccttgtt gggttcgaac
//
LOCUS       DATAQL010000637         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2049536, whole genome shotgun sequence.
ACCESSION   DATAQL010000637 DATAQL010000000
VERSION     DATAQL010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049536"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..968)
                     /locus_tag="VHS97_11175"
     CDS             complement(48..968)
                     /locus_tag="VHS97_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448808.1"
                     /translation="MTFEKKFRAMNRRRFVPSTEGLEGRALQASTGLNVFGLQLTNNL
                     NVPITFEQKAKRIENLPFFMGQIHPGRFLPKAEIQQIQGALFNMIDSIQKPVPQVLDN
                     FNFQIRQIVPKQSLSAGDIAVLNHGVAGVLTNAKAPAASVEGISNALQKLISQVDTAS
                     VMPVYVGTNDATIVLQTALAIGRPMPPPQLPKIKRNTGIQADAQHIKTPLERPVLVGT
                     YHFHTTMQVVTPDGVVVGQGVTARNNDYRVQITTPQTVGVHEFRIQAVDTVGHVSRIS
                     PPFLIKIVPTQHQDTAIGKATPQGPLATTK"
     gene            complement(1086..2030)
                     /locus_tag="VHS97_11180"
     CDS             complement(1086..2030)
                     /locus_tag="VHS97_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3448809.1"
                     /translation="MGTVLVLWLTVSFAVAYRLTHRPRPRFEERAPRVAWGTLEDHRI
                     KTRDGEELGAWFLKGDDDLPIVLLLHGNKGSRWNSLTRAEIFASQGWGVLMISLRAHG
                     DSSGDFHDVGLSARRDVVAAVEFLEARRPGRPIVVMGTSLGAATAVFAAEELGQRVKG
                     YILESPYKDLKTAVWNRTDTYLPPALSHAGYLGLRAVGPLFLPNLDEISPLKAIGGIP
                     NDVPVLISRKTGRARQIEASKGTSTSSATQRKETKNRPCCYPKDARSEPGRFLLLLNN
                     VNRYFPCIFAFRSVLTRCKSRFRIPLSPFELVRPRRLW"
     gene            complement(2117..>2377)
                     /locus_tag="VHS97_11185"
     CDS             complement(2117..>2377)
                     /locus_tag="VHS97_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX3448810.1"
                     /translation="RTPYHLTRDGRRIEVVLDRAEGLGDFVEIETLAATETDLPAAQA
                     AVLALASELGLTEVEPRSYLRMWLEANEKKGMEGATKTSSAR"
BASE COUNT          467 a          685 c          712 g          513 t
ORIGIN      
        1 gacaacaggc aactttgctc agagcgagtg ccggcgcgct tcgatcctca ctttgtggtc
       61 gcgagaggtc cctggggcgt ggccttgccg atggcggtat cttgatgctg cgtgggtacg
      121 atcttgatca agaatggcgg actaatgcgg cttacatgtc caaccgtatc gactgcttga
      181 atccgaaatt catgcacgcc cacggtctgc ggcgtcgtga tctggaccct gtagtcgttg
      241 ttcctggcgg tgacaccttg gcccactacg actccgtcgg gcgtgacgac ctgcattgtg
      301 gtgtggaaat gatacgtgcc aacgaggacc gggcgctcaa gcggcgtctt gatgtgctga
      361 gcatccgcct ggatgccggt gttacgcttg attttcggga gctgcggcgg gggcatgggc
      421 ctgccgatcg ccagggcggt ttgcaggacg atggtcgcgt cgttggttcc cacgtacacc
      481 ggcatgacgc tggcagtgtc tacctgcgag atgagcttct gaagcgcatt cgagatgcct
      541 tcgaccgacg ccgccggagc cttcgcattg gtgaggaccc ccgcaacccc gtggttcaga
      601 accgcaatgt cgccggccga aagcgactgc ttgggaacga tctggcgaat ctgaaagttg
      661 aagttgtcaa gcacctgggg cacgggtttt tggattgagt cgatcatgtt gaacaaggca
      721 ccctggatct gctggatctc ggccttgggc aggaaccgcc ccgggtgaat ctggcccata
      781 aagaacggga ggttctcgat tctctttgct ttttgctcga acgtgatcgg cacgtttaaa
      841 ttgtttgtaa gttggagccc gaagacgttg agtcctgtcg aggcttgcaa ggcgcgacct
      901 tccaatcctt cggttgaagg aacaaaccgg cggcgattca tcgcgcggaa cttcttttca
      961 aaggtcacgc ttcagtcctg cggatatggt tcatggcgga cggtcgaccg tcgagtcgtt
     1021 aattcggcga tcgagtccgc gaacaaaaga tgtgttcgca tcccgtccct gggagcgtgg
     1081 agttcttacc acaaacgccg tggtctgact agctcgaatg ggctgagcgg gatgcgaaac
     1141 cgcgacttgc accgtgtgag gaccgaccgg aaagcgaaga tacaagggaa ataacgattt
     1201 acgttattca aaagtagcaa gaatcggccc ggttcagaac gcgcgtcttt cgggtaacag
     1261 cacggacgat tcttcgtctc cttcctctgg gtagcgctgg aagtagaggt ccccttgctc
     1321 gcttcgattt gtcgggctcg tccggtcttg cggctaatca ggacaggcac gtcgttagga
     1381 attccgccaa tcgccttcag aggcgaaatt tcatccaggt tgggcagaaa cagcgggccg
     1441 acggctctca atcccaaata gccggcatgg ctgagcgccg gcgggagata ggtatcggtt
     1501 cgattccata ctgccgtttt caagtcctta tacggacttt ccagaatgta gcccttgacg
     1561 cgttgcccga gctcctccgc ggcgaacacc gctgtggccg ctcccagcga cgtccccatg
     1621 accacaatgg gccgtccagg gcgccgggct tccagaaatt cgaccgccgc caccacgtcg
     1681 cgccgcgcgc tcaagcccac gtcgtgaaaa tcgcccgacg agtcgccgtg agcgcggagc
     1741 gagatcatca gcacacccca gccctgggac gcaaagatct cggcccgcgt gagactgttc
     1801 caccggctcc ctttattgcc atgaagcaaa agaacgatcg gtaaatcgtc gtcgcctttt
     1861 aagaaccagg cgccgagttc ctctccgtcc cgcgtcttga tacggtggtc ttcgagtgtt
     1921 ccccatgcga ctcgcggtgc tcgctcttca aatcgtggtc gaggacgatg ggtgagccga
     1981 taggcaactg caaatgatac cgtcagccac aaaaccagaa cggtgcccat cgcgatcgcg
     2041 ccacgtcgga accatttccg agaacctgat cgcaggaacc cattgagtgc gggaacaaac
     2101 aaacgaccat caatttctac cgggcggacg atgtcttcgt agcaccttcc atcccctttt
     2161 tctcgttggc ttcgagccac atccgtagat aggatcgggg ctcgacttcg gtcaggccca
     2221 gttcgctggc cagtgccagc acggcggcct gggcggcggg cagatctgtt tcggtggccg
     2281 cgagcgtctc gatctcgacg aagtcaccca gtccttcggc tcggtcgagc acgacttcga
     2341 tgcgccggcc gtcgcgggtg aggtgatatg gcgtccg
//
LOCUS       DATAQL010000638         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_676835, whole genome shotgun sequence.
ACCESSION   DATAQL010000638 DATAQL010000000
VERSION     DATAQL010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676835"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="VHS97_11190"
     CDS             complement(<1..1812)
                     /locus_tag="VHS97_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3448811.1"
                     /translation="MDEGRARVYDDLRGVVRGDLYFEPLERAPYAHDASLYEIDPLGV
                     VVPRDEDDVVSAVRYASENHIPVHLRGAGTDTGGGSLGPGLVVDVSRHLRKIIAIGPE
                     HVVVEPGVVLDVLNSQLAPLGRRLDPVPSDSDVTTVGGMIGVDAAGARSMRHGSTGDQ
                     VERLRAVFAQGEVVDLGFETWPKFEAEPSDSKDLIVRKLQTLYRRSQARLARLAPAVP
                     RNRAGYGLLKAASESGIHLGRLVAGSEGTLAIVLQAALRTVPLAAATGVVVLPFVGLS
                     DAAVFVPQVLDSALQVTSCDLFDRRSLCLAREADLAFRGWIDEAAEAILTVEVEGDDS
                     DSVAGKIRLLAERAARMRVLAAEPFATVKRSECDRLLGLRRLLEPLLMRSRGRARPVS
                     YIDDIAVPPDRLAPVLQRLQSLLQQSNLTWTLDAFAGEGRLRIRPMLDLADPGDRAKL
                     EPLASRVYDIVIEAGGTISSAQGCGLVRTQFMRKQYGELVQVFREIKDAFDPQNQLNP
                     GKVIGDDAHLMLRDLKPSAPAIAQAITDPPSSLFSSGNGEVAAQTWGQTAADQLGAAS
                     APDDTKLPPQATVIEPALRWPGLTLIETASACHGCGTC"
BASE COUNT          384 a          717 c          638 g          363 t
ORIGIN      
        1 gcaagttccg caaccatgac aggccgacgc ggtctcgatg agcgttagac ccggccaacg
       61 tagcgccggc tcgatcacag tcgcttgggg gggcagcttg gtgtcgtcgg gcgcgctggc
      121 ggctcccaac tggtccgccg ccgtctgccc ccacgtctgt gccgcgacct caccgttgcc
      181 cgacgaaaat agcgacgacg gtggatcagt gatcgcctgg gcgattgccg gcgcagatgg
      241 cttcagatct cgtagcatca ggtgggcgtc gtcgccaatg accttgccgg gattgagctg
      301 attctgcggg tcgaacgcgt ctttgatctc ccggaaaacc tgaaccagct cgccgtattg
      361 cttgcgcata aactgagtgc ggaccaggcc gcaaccctgg gcgctcgaga ttgttccacc
      421 ggcttcgatg acgatgtcgt acacgcggct cgccaacggt tccagcttgg cgcggtctcc
      481 gggatcagcc agatcgagca tcggtcgaat ccgcagtcgg ccttcgcctg cgaacgcgtc
      541 gagagtccag gtcaggttgg actgctggag caggctttgc aaccgctgca agaccggggc
      601 gagccggtcg ggcgggaccg cgatatcgtc aatgtacgag accgggcgcg ccctaccgcg
      661 cgaccgcatc aagagcggtt ccaagagtcg ccgtaggccg agcagccgat cgcattcgct
      721 tcgtttcacg gttgcaaacg gttcggctgc caggaccctc atacgtgccg cgcgttcggc
      781 tagcaagcgg atcttgccgg cgacggagtc cgagtcgtcg ccttcaactt cgactgtgag
      841 aattgcctcg gcggcttcat cgatccagcc gcgaaacgcc aggtcggcct cgcgagccag
      901 gcagagcgac cggcggtcga ataggtcgca cgacgtgact tgtaacgcag aatccagcac
      961 ttgcgggacg aacaccgccg cgtctgacaa tccgacaaag gggagcacca caactcccgt
     1021 agccgcggcc agcggaaccg tacgcagcgc cgcctgcaac acgattgcca gtgttccctc
     1081 ggaaccagcc accagacgac ctaggtggat gccagactcg ctcgcggctt tcaagagccc
     1141 ataacccgcc cgattccgcg gtacggccgg cgccagccgc gcgagcctgg cctggctccg
     1201 tcgatacaga gtctggagct tgcggacgat cagatctttc gagtcgctcg gctcagcctc
     1261 gaatttcggc cacgtctcga accccaggtc gaccacttcg ccctgggcaa acacggcgcg
     1321 taggcgttcc acctggtcgc cggttgagcc gtggcgcatc gaccgagccc ccgcggcatc
     1381 gacgccgatc attcccccca cggtggtgac gtcgctatcg ctcggaaccg ggtccagccg
     1441 gcgccccaag ggcgccagtt gagaattcag cacgtcgagc acgacccccg gctcgaccac
     1501 cacgtgctcc ggaccgatcg cgatgatctt gcggagatgg cggctcacgt cgacgaccag
     1561 acccggaccc agcgaccctc cgccggtgtc agtccccgcc ccccgcaaat gcaccggaat
     1621 atggttctcg gatgcatacc ggacggcgct cacgacgtcg tcttcgtcgc gcggaacgac
     1681 tacgcccaga ggatcgatct cgtacaaact cgcgtcgtgc gcgtagggag ctcgctccag
     1741 gggctcaaaa taaaggtcgc cccggaccac tccccgcaag tcgtcatata cccgagcgcg
     1801 gccttcgtcc acggcggtgg cagcacctca agtgttcatt gaccgcaagg ttccgaggct
     1861 acatccgatc cagatcgagg caaaggcgcc ctatccatgc tcgttaccgg taagggcgcg
     1921 ccgcccgtac cgtcgcggcg tgatggccct ttcgttcatt ccgcctgtgc gccgcgttct
     1981 cgcagctgct cggcgcgaat tcgctcttgc cgataccgtt cgccgatagt gaggtcgaat
     2041 ggacgatacc ggccgtcgcg attagaccct atcccccgca actggcacaa gcatcgatag
     2101 ca
//
LOCUS       DATAQL010000639         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4188445, whole genome shotgun sequence.
ACCESSION   DATAQL010000639 DATAQL010000000
VERSION     DATAQL010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4188445"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VHS97_11195"
     CDS             <1..497
                     /locus_tag="VHS97_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3448812.1"
                     /translation="PYEFEGTYVLPESQLDRFMLRLRMGYPIRSEERKVFASHRNGEP
                     VEMLTPVLTAQEVLQLQHGVRGVQVDDAITEYLLDIVHATRRSEDLHVGVSTRGALTL
                     YRAAQSLALVSGRNYVVPDDIKLLVVPVLAHRVVGKSFLQAGEFGAAEAIIHDLVDRI
                     RVPA"
     gene            555..1955
                     /locus_tag="VHS97_11200"
     CDS             555..1955
                     /locus_tag="VHS97_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3448813.1"
                     /translation="MSVNGKPRARGRRGGIRRFARKVLGAVLPTERQILTREGFIYFT
                     GGVLLLFAGLYQQVNLILLVFTLSAGPFLASIFGGRAMLRRLSVARRVPSYVFSGDPL
                     VLDYTLENGRRWFASLAVFLEDSLVSADRSATGNVSLTPRVFFARVAGGDRVRMRWES
                     KSPRRGKYRFRDLDLGTRSPFGLVEHRVTISLADQILIYPTIGRLTRRWFQLQRQATE
                     NRRGQRHDRSAMQMEYHGLRDYRSGDSPRWIHWRTSARRGELMVKEFEQQNEQDLAIL
                     IDPWLPRSKAAPEQREALEQAISFAATLCLETCRHQGRRLVLGWTGAAPGVRKGPASV
                     KLLHELLEQLAVMKPASEGGLAELFDVMPPAVLREALMVVISCRPLNLIEEAERSSRL
                     SGATARSLLGRVVLLNSTQGDLAPLFQLADASTRNLLEYRVSSAVEERLSSQGQRRRG
                     GGSDDSSTDLDRSSFD"
     gene            1970..>2791
                     /locus_tag="VHS97_11205"
     CDS             1970..>2791
                     /locus_tag="VHS97_11205"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX3448814.1"
                     /translation="MNTYLVYRASFYLMLVVANLALVGDAVDGQYAMIYTLAVAVAGA
                     VAFFTVDLNQRWALPRGLANALGVATLGILFLEYKNNENQMIQALGHWLIYLQLVKYF
                     LPKTAEDDWFLFVLGLMQVLIGSVVNQSDHVGSWLFLWAMLAVWVLGQFFLQREARRL
                     LPASNGPVDHAMSAPLVDPYRGLFDVPYVMATIRVLATTLALGGLIFLVLPRRAGATR
                     NQGAPPMSRHLTGFDEEVQLGQLGEILENDTVVMTVEFTDEQHETIRPVGEPLWRG"
BASE COUNT          482 a          838 c          879 g          592 t
ORIGIN      
        1 acccctatga attcgaagga acctacgttc tccctgagag ccagctcgac cgcttcatgc
       61 tccggctgcg gatgggatac ccgatccgct ccgaagaacg caaggtcttt gcgagtcatc
      121 gcaatggcga acccgtcgag atgctaaccc cggtcttgac agcgcaggaa gttctccagc
      181 ttcagcacgg cgtgcgagga gtccaggtgg atgatgcaat caccgagtac cttctcgaca
      241 ttgtgcatgc gacgcgccgc agcgaagacc tgcacgtggg ggtgagcacg cggggcgcct
      301 tgacgctcta tcgggccgcc cagagcctcg cactggtttc aggtcgcaat tatgtggttc
      361 ccgatgacat caaattactg gttgtaccgg tactggctca ccgcgttgtg ggtaagtcgt
      421 tccttcaagc gggagagttc ggagcggccg aggcgattat tcacgatctg gtcgaccgca
      481 ttcgagtgcc ggcgtgaaaa gtcaggctcg ccgcaaacat ttgttggctc cgcggggcgg
      541 gaggctctag atcaatgagc gttaacggca aaccgcgcgc gcggggacgg cgtggcggca
      601 tccggcgttt cgcccggaag gtgttaggcg ccgttttgcc cacggaacgc caaatcctga
      661 ctcgcgaggg tttcatttac ttcaccggcg gagtgttgct ccttttcgcg ggcttgtatc
      721 agcaagtcaa tctgatcttg ctggtgttca ctctttcggc gggcccgttc ctggcttcga
      781 tcttcggtgg ccgggccatg ctgcgccggc tgagcgtagc gcgccgcgtg ccgtcgtacg
      841 ttttttccgg cgatcctttg gtgcttgact acactctgga aaatgggcgg cgctggtttg
      901 cctcgctcgc ggtgttcctc gaagattccc tggtctcggc cgatcgctcg gccacgggaa
      961 atgtgagtct cacgccccgg gtttttttcg cgcgggtcgc aggtggcgat cgtgttcgca
     1021 tgcgctggga aagcaaaagc ccccggcgcg gcaagtatcg gtttcgtgac ctcgacctgg
     1081 gcacccggtc ccctttcgga ctggttgaac atcgtgtcac aatttcgctc gccgaccaga
     1141 ttctgatcta tcccacaatc ggccgtttga cgcgccgctg gttccagcta cagcggcagg
     1201 ccaccgaaaa ccgtcgcggc cagcggcacg accgatcggc gatgcaaatg gaatatcatg
     1261 gactgcgcga ttaccgttcg ggtgacagcc cgcgctggat ccattggcgc acgtcggcgc
     1321 ggcgcggcga gttgatggtc aaggagttcg agcagcaaaa cgagcaagat ctggcgatcc
     1381 tgatcgatcc ctggctaccg cgtagcaagg ctgcacctga acagcgtgag gccctcgagc
     1441 aggcgatctc gttcgcggcg accttgtgtc tggagacgtg ccgccaccag gggcgccggc
     1501 tggtcttggg ctggaccggc gcggcaccgg gtgtgcggaa gggtcccgca tcggtcaagc
     1561 tgttgcacga actcttggag caactcgccg tgatgaagcc ggcgagcgag ggggggctgg
     1621 ccgaattgtt cgacgtcatg cctccggccg tcttgcgcga ggcactgatg gtcgtcatct
     1681 catgccggcc cttgaacctg atcgaagaag cagaacgatc gtcgcggctc tcgggcgcaa
     1741 cggcccgcag cttgctcggg cgagttgtct tgctcaacag cacacagggg gacctcgctc
     1801 ccctgtttca gctcgcagac gcctccactc gtaatctcct ggagtatcgc gtcagcagcg
     1861 cagtggaaga gcggctctcg agccaggggc aacgccggcg cggtggcgga tccgacgatt
     1921 ccagcacgga tcttgaccgg tcgtcgtttg actgaggagc aatcgggtta tgaacaccta
     1981 tctcgtctac cgcgccagtt tttacctgat gctggtcgtc gccaacctgg ctttggtcgg
     2041 cgacgcggtg gacggccaat acgccatgat ctacaccctg gccgtggctg tggcgggagc
     2101 cgtcgccttc tttacggtcg atctcaacca gcgatgggcc ctgccacgcg ggctcgccaa
     2161 cgcacttggc gtggcgacgc tcggcatact cttcctcgag tacaagaaca acgagaacca
     2221 gatgatccag gccctggggc actggttaat ctacctacaa ctggtgaaat acttcctgcc
     2281 caagacggcc gaggacgact ggttcctctt tgtactcggt ctgatgcagg tcttgatcgg
     2341 ttcggtggtc aatcagagcg atcacgtcgg ctcttggctt ttcttgtggg ccatgctggc
     2401 ggtctgggtt ctcggccagt ttttcctgca gcgcgaggct cgacgactct tgccggccag
     2461 taacggaccg gtggatcatg ccatgtcggc gccgctcgtc gatccgtatc gcgggctgtt
     2521 tgatgttcca tacgtaatgg cgacaatccg agtcttggcc acgaccctcg cgctgggtgg
     2581 cctgatcttt ctggtcttgc ctcgccgggc aggcgcaacc cgcaatcagg gggcaccccc
     2641 catgtcccgc cacctgaccg gttttgacga ggaggttcag ctcggtcagc ttggcgagat
     2701 tctcgaaaac gataccgtcg tgatgaccgt cgaattcacg gacgagcaac acgagacgat
     2761 acggccggta ggtgagccgc tctggcgggg c
//
LOCUS       DATAQL010000640         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_827365, whole genome shotgun sequence.
ACCESSION   DATAQL010000640 DATAQL010000000
VERSION     DATAQL010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827365"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1664
                     /locus_tag="VHS97_11210"
     CDS             <1..>1664
                     /locus_tag="VHS97_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3448815.1"
                     /translation="IAGQVRELSRIYVDAVSGRNGQKLWHWRTDLNHGDTTPVGSPFW
                     WGRRPDGWPLLALPIGGTKAPERFAKNPYFTPDPPVVHLLAAATGREAHVVEGLSRPG
                     TADLDGDGLADLWGAVDGKVRAFRALAPEAWRALGGFQPAGDLDGDGVSDVVSNDLKA
                     PQVGRDPKPDSGTAIARSGRDGRILWQALLDPWENRFFWGDWAVSYGVEPLKVPAGDL
                     DGDGAPDIVVHRSTSWTHNNQRRAGIRLQALSGRTGRTLWPAGALPYVDSNIGIPYVA
                     GIDAAACKGWGRSDVFVLHHNFVKQSSIPPFTFSVQSRLARLSSRDGRLIWDVLLAEY
                     KGGMKSRRDFVYEFADLDGDGSLEMVLLFNGKAPAGTVPFEVRVLSLATGDTRWSHPL
                     NPRAVGSPTCVVGDLDGDKRPEVVVSEQPIVQGLPLTEVTALDGETGTPRWVWRGSAG
                     RDVTYQDVALVLADFDGRGRREVCVSFGVAPECRRVEILDSQGRSRGARDTESVNLAE
                     LINVDLDGDGRDELLFRDAGRLCAVRGDLTEIWSWPTREPIREVLP"
BASE COUNT          270 a          521 c          549 g          324 t
ORIGIN      
        1 catcgctggg caagtgcggg agctgagccg aatctacgtg gatgccgttt ccggtaggaa
       61 cgggcagaaa ctctggcact ggcgcaccga cctcaatcat ggagacacga cccccgttgg
      121 ttcgccattc tggtggggcc gcaggcctga cggctggccg ttgctcgctc taccaatcgg
      181 cggcaccaag gcgcccgaga ggtttgccaa gaatccgtac tttactcccg atccacctgt
      241 cgttcacttg ctcgccgcgg cgacaggccg cgaggctcac gtcgtcgagg gcctttcgcg
      301 gcccgggacg gctgatctcg acggcgacgg actcgcggat ctctggggtg cggttgacgg
      361 caaagtccgc gcgtttcgcg ctctcgcgcc cgaggcatgg cgtgcgctcg gtgggttcca
      421 acccgccggc gaccttgacg gcgacggcgt cagcgacgtg gtcagtaacg acctcaaggc
      481 accgcaagtc ggtcgtgatc caaagcccga cagcgggacg gccatcgccc ggtcgggtcg
      541 cgacggccgg atcttgtggc aggcgctgct tgatccctgg gagaaccggt ttttctgggg
      601 cgattgggca gtgtcttacg gtgtcgaacc tttaaaagtt ccagcgggcg acctggatgg
      661 cgatggggct ccggatatcg tagtccaccg gtcaacttct tggacccaca ataatcaacg
      721 tagggcgggt atccgcctgc aggcgctctc gggccgcacg ggccgcacgc tctggccggc
      781 gggagccctg ccgtatgtgg actcgaatat tggcatccca tacgttgcag gcatcgatgc
      841 agccgcatgt aaaggctggg gccgctccga cgttttcgtg ctgcaccata acttcgtcaa
      901 gcagagctcg atcccgccct ttaccttctc tgtgcaatca cgattggctc gcctctcgag
      961 tcgagacgga cgcctcatct gggacgtcct ccttgccgag tacaagggcg gaatgaagag
     1021 ccgccgggac ttcgtctacg agttcgccga ccttgacggc gatggcagcc tggagatggt
     1081 gcttttgttc aatggcaagg ctcccgcggg tacggttccg ttcgaggtgc gagtcttgtc
     1141 gctcgccacc ggtgacacgc gctggagcca tccccttaac ccacgcgccg tcggctcccc
     1201 cacatgcgtg gtcggcgacc tcgatggcga caaacgtccg gaagtggtcg tgagtgaaca
     1261 accgattgtg cagggcttac ccctgaccga ggttaccgct ctcgacggtg agaccggcac
     1321 gcctcgttgg gtttggcgcg gtagcgccgg gcgcgatgtg acgtaccagg acgtggcgct
     1381 tgtgctggcc gacttcgatg gtcgcgggcg gcgcgaagtc tgtgtcagtt tcggcgtggc
     1441 gccggaatgc agacgcgtcg agatcctcga ctcccagggt cggagtcgag gcgcccgtga
     1501 caccgagtcc gtcaatctgg ccgagctcat caatgtcgat ctcgatggcg acggccgcga
     1561 cgagctgcta ttccgcgatg cgggccggtt gtgcgccgtc cgcggcgacc tcacggagat
     1621 ttggtcgtgg cctactcgcg aacccatccg cgaagttcta ccgg
//
LOCUS       DATAQL010000641         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_370321, whole genome shotgun sequence.
ACCESSION   DATAQL010000641 DATAQL010000000
VERSION     DATAQL010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370321"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VHS97_11215"
     CDS             <1..1041
                     /locus_tag="VHS97_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448816.1"
                     /translation="TWMTGFVRDSRARFVTRGPAIAVVLAASLALVGSYTVVKGEISE
                     KLAFRLGAALGPKKPLVTRGPHHLPQGLDNPAWDFSPKEETDRIPIRNWRIAVLRIVG
                     KWWEELCWLFAVMTVWGIVRQRYIRGLCQDRDPDYAGDVESRLLLAFSAVYALALVRH
                     SALLGYLSGRHILAIVYASIPWAAAGSFVCARGIMVKLRFDARKVRIAGTLAGCVLVA
                     ASILVQMQPNHLNHLSRWGHWAAGQWLAGHADSTELVLDTRGWARFVSDHPGYDYWHV
                     RQALTDSHLSYIIVGLDELEAKSSRAKTLNALLSYAATPLKDFPAFPGDRTAGVRLYR
                     FQRPDTWEGFVP"
     gene            1038..1952
                     /locus_tag="VHS97_11220"
     CDS             1038..1952
                     /locus_tag="VHS97_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX3448817.1"
                     /translation="MSYGSFWQRWSRGVSWTWINEQYRARLPEDLSDNVMTLDSPDRF
                     HAKQGRSTARVVLHRADKPLAVYLKRHYRLPFVARLRALFDPGGKHSPAAAEWSHLER
                     ARALGIDVPEVVAVGERIGPTGHLKSFLMIVELAGCQAVNELLPRLAVELDAHAFGTL
                     KRRLIAEMARITAALHMARVFHKDLYLCHFFLDHDRLDWERGTAQVSLIDLHRLGEHR
                     LWPDWWRWKDLGQLLYSTEGVDGITDRDRLRFWRSYCRKLGIGLPRWHAWVIRLRAAR
                     YLEHNRKPPYRQTPPEPESLTAGQGEER"
BASE COUNT          406 a          674 c          694 g          435 t
ORIGIN      
        1 acgtggatga cgggattcgt tcgtgactcg cgtgcgaggt tcgtgacgcg cgggccagcg
       61 atcgccgttg tcctcgccgc ctcgctcgcg ttggtgggat catacaccgt ggtcaaggga
      121 gagatctccg agaagctggc attccggctc ggcgccgcgc tcggacccaa aaagccgctg
      181 gtcactcgcg gcccccacca tctcccgcag ggactggata accccgcgtg ggatttctcc
      241 cccaaagaag aaaccgaccg gatcccaatc cgcaactggc gaattgcggt gctgcggatt
      301 gtcggcaagt ggtgggaaga gttgtgttgg ctgtttgctg tcatgactgt ctggggcatc
      361 gtgcgccagc gctatatccg tggcctctgc caagatcgcg accctgacta cgccggcgac
      421 gtagaaagcc ggctgctcct ggcgttctcc gccgtgtacg cgctcgcact cgtgcggcac
      481 agcgcacttc tgggctatct gtcggggcgt cacatcttgg cgatcgtgta cgcgtcgatc
      541 ccgtgggcgg cggccggaag cttcgtgtgt gcgcgcggaa tcatggtcaa gctaaggttc
      601 gacgctcgaa aagttcgaat tgcgggcact ctggccggct gcgtgctggt ggcggcctcg
      661 atcttggttc agatgcaacc gaaccacttg aaccatctca gccggtgggg tcactgggcg
      721 gccggacaat ggctggccgg ccatgccgac tcgaccgagt tggtactcga cacgcgcggc
      781 tgggctcgat tcgtctcgga tcatcccggt tacgattact ggcatgtacg ccaggccctc
      841 acggactcgc atctgagtta catcatcgtg ggattggacg agctcgaggc caagagctcg
      901 cgtgccaaga cgctgaacgc tttgctctca tacgcggcaa ctccgctcaa agacttccct
      961 gcctttccgg gcgaccgcac agccggagtc cgcctctatc gctttcaacg gcccgacact
     1021 tgggaaggat tcgtcccgtg agctacggct cgttctggca gcgatggtcc cgcggtgtga
     1081 gctggacctg gatcaacgag cagtatcgcg cccgcctgcc cgaagacctg agcgataacg
     1141 tcatgacgct cgacagcccc gatcggtttc acgctaaaca gggccggtcg accgcacggg
     1201 tggtcttgca cagggcggat aagccactgg cggtgtactt gaagcggcac taccgacttc
     1261 cgttcgtcgc gcggctcaga gcccttttcg accccggcgg caagcattcg cccgccgcgg
     1321 ccgaatggtc gcatctcgag cgcgcacggg cgctggggat cgatgtaccc gaagtcgtgg
     1381 ccgtcggcga acgaattggt ccaacaggcc acttgaagag tttcctcatg atcgtagagc
     1441 tagccggctg ccaagccgtg aacgagctct taccccgatt ggccgtcgag ctcgatgccc
     1501 acgcctttgg aaccttgaag cgccggctaa tcgccgagat ggcaaggatc accgcggccc
     1561 tgcacatggc tcgtgtcttt cataaggatc tgtatctctg ccactttttc ctcgatcacg
     1621 atcgtctcga ctgggagcga gggacggccc aggtcagctt gatcgacctg caccggctcg
     1681 gggaacatag gctctggccc gattggtggc ggtggaaaga cttgggccag ttgctctact
     1741 cgaccgaagg cgtcgacggc atcactgacc gcgaccgtct taggttttgg aggagttact
     1801 gccgaaaact cggaataggc ctgccgcgct ggcatgcgtg ggtcatccgg ctcagggcgg
     1861 cgcgttatct ggagcacaat cgcaagccgc cttacaggca gaccccacca gagccggaat
     1921 cactcacggc aggacagggc gaggagcgat aagacgatga tggtggaatg agactgctct
     1981 aatccattcg ataatcagac atcaggtcgg cattactcgg ctccgcaatc cgattccagc
     2041 gagagggaga ttagagacca tgcaccttgc tctgaacttt cagcggatcg atcccacgcg
     2101 gggaggcgcc gagacgtata tcgccgatct gtgccggcaa ctggttggag ccgggcaccg
     2161 cgtggacctt tatgccgaga gctgtgccga cggctgcttg ccgcaaggg
//
LOCUS       DATAQL010000642         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1898022, whole genome shotgun sequence.
ACCESSION   DATAQL010000642 DATAQL010000000
VERSION     DATAQL010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898022"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1980)
                     /locus_tag="VHS97_11225"
     CDS             complement(<1..>1980)
                     /locus_tag="VHS97_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX3448818.1"
                     /translation="RRGGARRDASPIKPTTGLGPLPLSFSQEALWFLDQLTPGQPTFN
                     VTAALRIKGPLDHGALERSINELGRRHEALRTSFVATGGTPQQVVDLTASLSLDTTDL
                     TELPLGLREDEARRRALDESRRPFDLTQGPLAHVSLLRLGDADHAVLFTMHHLITDGW
                     SFGVAASELATLYEADRHGLPSPLPDPPIQYADFARWQRDQFESGAWSAQIECWRRRL
                     GGVPALELPTDRPRPPIRSARGALHHLVLSPELSAMVRTVGLREGVTPFMTLLAAFQL
                     VLGRWSGQDDFAIGAPAANRNRPETERLIGYFVNMIALRADLSGNPTVREFLARVREV
                     ALEAFENQEVPLEILIPALAPRRDASRSPLFQVMFVLQNNAMPAVDQLDLAISPLNRD
                     QGTGTSKFDLSLGFEDTPGGFTGSVEFNTDLFKGETIERFSHQYVKALEYLIVQPERR
                     LSELSLLTDAEREQVVAWSHARSDTAQGVGIPDRALPSAIHGAFEAQVRAAPDDLALV
                     AGAESLTYAELNARANRLAHYLRSRIAGPEVRVGLILDDPIHRIVAVLGVLKAGGAYV
                     PLEPSLPRVRLEGMMDVAAVDIVIVDRGALELSPRTSATMINLDAEESSIAAQSPEDP
                     SVPVDSENLAYVVFTSGTTGRPKGVMVSHRSLRGAA"
BASE COUNT          356 a          625 c          680 g          319 t
ORIGIN      
        1 gcggcaccgc ggaggctacg gtgggaaacc atcacgccct tgggccgccc ggttgtgccg
       61 gaggtgaaga cgacgtaggc gaggttttcg ctgtccactg ggactgaggg gtcctcggga
      121 ctctgggcgg cgatcgacga ctcctcggcg tcgaggttta tcattgtcgc cgaggttcgc
      181 ggcgacagtt cgagcgcccc gcggtcgacg atcacgatgt cgacggctgc aacgtccatc
      241 atgccctcca gccgcacccg tggcagagaa ggctccaggg gcacatacgc gccgcccgcc
      301 ttgaggacgc caagtactgc cacgatccga tgaattggat cgtcgaggat caggccgacc
      361 cggacttccg ggccggcgat ccgcgaacgg aggtaatgcg ccagccggtt cgcacgcgcg
      421 ttcaattcgg cataggtcaa actttccgcg ccggcgacca gggcgagatc gtcgggtgcc
      481 gcgcggacct gggcctcgaa cgcgccatgg atggctgacg gcagagcccg atcggggatg
      541 cccactcctt gcgcggtgtc cgaccgcgcg tgactccacg ccacgacctg ctcgcgctcc
      601 gcgtcggtga gcagcgacag ctcggaaagc cgccgctctg gctgaacgat gaggtactcg
      661 agcgctttta catactggtg cgagaacctc tcgatcgtct cgcccttgaa caggtcggtg
      721 ttgaactcaa ccgagccggt gaacccacca ggcgtgtctt cgaaccccag cgagagatcg
      781 aacttcgacg tgccggtccc ctggtcacga ttgagtggag agatggccag gtcgagctgg
      841 tcgacggccg gcattgcgtt gttctgcagg acaaacatca cctggaagag cggagaacgg
      901 ctggcatcac gccggggcgc aagcgcggga atgaggatct cgagcgggac ctcctggttc
      961 tcgaatgcct ccagggcgac ctcacgcaca cgggccagga attcccgcac ggtcggattc
     1021 ccggatagat cggcgcggag cgcgatcata tttacaaagt agccgatcaa tcgctcggtt
     1081 tccggcctgt tacggttggc cgccggcgcc ccaatcgcga agtcgtcctg gccgctccag
     1141 cggcccagca caagttggaa ggccgcaagc agggtcatga aaggagtcac gccctcacgg
     1201 aggccgacgg tccggaccat cgccgacaat tccggggaga gcacgaggtg atgaagggcc
     1261 ccccgcgcgc tccggatcgg tggacgaggg cgatctgtcg gcagctcgag tgccggcact
     1321 ccgccgagtc ggcgcctcca gcactcgatc tgggccgacc acgctccgct ctcgaactgg
     1381 tcgcgctgcc agcgggcgaa atcggcatac tggatcggcg ggtccggcag cggcgacggg
     1441 aggccatggc gatcagcctc ataaagggtc gctagctcgc tggccgcaac gccgaacgac
     1501 cagccatcgg tgatgaggtg gtgcatcgtg aagagaactg cgtggtcggc gtcgcccagt
     1561 cgcagcagac tcacgtgagc aagtggcccc tgcgttaagt cgaagggccg ccgcgactca
     1621 tcgagcgctc gacgccgggc ctcatcctcg cgaagcccga gcggcagctc agtcagatcg
     1681 gtcgtatcca gtgataacga cgcggttagg tccacgacct gctgaggtgt gccgcccgtt
     1741 gcaacgaaag acgtccgcag cgcctcatgg cgacggccaa gctcattgat gctccgctca
     1801 agtgcgccgt ggtcgagcgg gcctttgatt cgtagggccg cggtgacgtt gaaggtgggc
     1861 tggccgggcg tgagctgatc gaggaaccag agagcctctt gggagaacga gagcggcagc
     1921 gggccgagcc cggtggttgg tttgatcggg ctcgcgtcgc gccgggcgcc gccgcgacgg
//
LOCUS       DATAQL010000643         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1135226, whole genome shotgun sequence.
ACCESSION   DATAQL010000643 DATAQL010000000
VERSION     DATAQL010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135226"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..672
                     /locus_tag="VHS97_11230"
     CDS             271..672
                     /locus_tag="VHS97_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448819.1"
                     /translation="MRDAMSGHRKLIHSPQAQATVMAESGVIYTCPDDLLERLPSLMA
                     RAAIAVEELPRPEYGEGCERGFALQFRRDQGTVELSGFYFVEENRFIMGFSWGRNPLR
                     WKWDEKLSGVVKELLMDGGMIRISTKTDENS"
     gene            790..1329
                     /locus_tag="VHS97_11235"
     CDS             790..1329
                     /locus_tag="VHS97_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448820.1"
                     /translation="MPLDNLLALCIEKWRRQGIGLLPPVEEVEVHRAWGSFGQQAAAD
                     VVRLYTTVGGFAGYEMADPFYWSLWDWDQLLDENTQRPGAGVMFSDHMLRSIDYELRF
                     EDELRTSVWHLGRFGPELPELVAPSLDMFFRLYLEDPWTLAGAWSPSEGAPSKMRGPK
                     RGGWVMEKSDPLWDELLDG"
     gene            1445..2722
                     /locus_tag="VHS97_11240"
     CDS             1445..2722
                     /locus_tag="VHS97_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448821.1"
                     /translation="MNCTILLGLVVVVTLAADDPAAPALPIEPPEQVEVLPVFVVPRG
                     EASPTTEQTRRLVEHVKLCQNRYEAMLGRRDTFRRAEGPMRVHRSGSTLAELRRLPED
                     SAPAIVGALLTESKLNRFNCPYVFLAVVINPGSDWPVGGGRPLNGGFNTGGGIVVLSS
                     RGLDRSPNFQSTLQHELGHGFGLPHVDVYGYDMKTNPSVMSYNLAHHTQGFRPSPTPG
                     RLIAEDIRGLALNRRAFPKLTFDPNRDAARSSRLEKLGWLGPMTIPGQPAYAPKLTTP
                     SGEDFGTAVGNVVAGRVESSAPPPAGAGGTAFNAKTMWQSKPSPDGWVTVEAAFPFEV
                     TLTGVAVHSQHSGRYHAADRLRVETPVARGDHPLAEFSLTSVDQQVALSPTTTRTWRF
                     RVHAANGRVVVLRGLRFFSSRGEIVPPLVPYGS"
     gene            3101..3649
                     /locus_tag="VHS97_11245"
     CDS             3101..3649
                     /locus_tag="VHS97_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448822.1"
                     /translation="MKSALRFILATILCGCLAAMANAQSNALKYGDGQADGKQSVGGS
                     GEMIEFALPRESPRIGGLRIHGSRYGMPEAPDESFLIFFLSRDRKSIVHTEMAPYSHF
                     DRGAEKWVTITFSRPVELPDHFWVVLDFRASQRKGVYVSYDTSSGGKHSRTGLPGTPA
                     LETKFGGDWMIELLPAKREAVR"
BASE COUNT          676 a         1186 c         1263 g          723 t
ORIGIN      
        1 cgccgcgcgg gtggtgatcc tgcaaggggt cgtcgccatg ggatgcgagg tcgaccccga
       61 gccggcggtc gactggcttc atgagcaagg catctggtcc aagtaagtcc cggcgaggtg
      121 gcattttttc agaatccctc ccctaccgac gaggagcgga tccgcttccg ctgacaccag
      181 gaggccgcgt gggcttggct ttgggcgatc ggcaaggttg atgcgttggg cctcccaacg
      241 cggggcggct cgtagttggc gcggggtcac gtgcgcgacg cgatgtcggg gcaccgcaag
      301 ttgatccata gcccgcaggc acaggcgacc gtcatggcag aatcaggggt tatctacacc
      361 tgcccggacg atttattgga gagactgcca tcactgatgg cccgtgctgc aatcgctgtg
      421 gaggagctgc cccgcccgga gtacggcgaa ggctgcgaac gaggcttcgc ccttcagttc
      481 aggcgtgatc aggggacggt cgaattgtcc ggattctatt ttgtggaaga gaatcgattc
      541 ataatgggtt tcagctgggg acggaatccg ctccgctgga aatgggatga gaaattgtct
      601 ggagtggtga aggaattgtt aatggacggc ggcatgatcc gcatttcgac caagacggat
      661 gaaaactcct agttcgcgtc ggccgatctg taggtggcgc gggcagtcgg gggcgggacg
      721 atgggtgaat gtggaacgga cggacaagaa gagataattg ccaatgcata cgcgcgaaga
      781 gatacgctta tgccgcttga taacctcctt gctctgtgta tcgagaaatg gcgccggcag
      841 ggaattggcc tcttgcctcc cgtcgaagaa gtcgaggtcc atcgcgcctg gggcagcttc
      901 ggccagcaag ccgccgccga cgtcgtccgc ctttacacca ccgtcggagg cttcgcgggt
      961 tacgaaatgg ctgatccgtt ctattggtca ctctgggact gggaccaact cctcgacgaa
     1021 aacacccaac gccctggtgc tggcgtgatg ttttccgacc acatgctcag atcgattgac
     1081 tatgagctcc gatttgagga tgaactacgg acttcagtct ggcatctagg acgtttcggc
     1141 cctgagctgc ccgagttggt cgctccaagt ctcgacatgt tctttcgcct ttacctcgaa
     1201 gacccatgga cgctggcggg tgcctggagc ccttctgagg gcgccccgag caagatgcga
     1261 gggccgaaac gcggcgggtg ggtcatggag aagtccgatc cgctctggga cgaattgctg
     1321 gatggctgaa gccagcgccc ccgttggcaa tgtatcgact cgcctctcgg aacaaggtca
     1381 aagcgcaacc caagggtccg cgttgcacgc cgggccttga cgaggcctca cgggagctcc
     1441 acgcatgaac tgcaccatcc tccttggtct ggtcgtggtc gtaaccttgg cggccgacga
     1501 cccggccgcc cctgcgctgc cgattgagcc gccggagcag gtagaggtgc tgccggtttt
     1561 cgtcgtgccc cggggggagg cgtccccgac gaccgagcag acccgccggc tggtagagca
     1621 tgtcaagctc tgtcagaatc gctacgaggc gatgctgggg aggcgcgaca cgttccggcg
     1681 cgccgaagga ccgatgcgag tccaccgctc gggctcgacc ctggccgagc tgaggcgtct
     1741 gcccgaggac tccgcacccg ccattgtcgg cgcgctcttg accgagtcca agctcaaccg
     1801 cttcaattgc ccgtacgtct tcctggcggt ggtgatcaac cccggctctg actggccggt
     1861 gggaggcggc cggccgctca acggcgggtt caacacaggc ggcggcatcg tcgtcctctc
     1921 gtcgcgcggg cttgaccgat cgccgaactt tcagtcgacc ttacagcacg agctcggcca
     1981 cgggttcgga ctgccgcacg tggacgtcta cggctacgac atgaagacca acccatcggt
     2041 catgtcgtac aaccttgcgc accacaccca ggggttccgg ccttcgccca cgcctggccg
     2101 gctgatcgcc gaggacattc gagggctggc cctgaaccgg cgggcgttcc cgaagttgac
     2161 cttcgacccg aaccgcgacg ccgcccggag cagccggctg gaaaagctcg gctggctcgg
     2221 gcccatgacg atccctggcc agccggcgta cgcccccaag ttgaccactc cgtcgggcga
     2281 ggactttgga accgccgtcg gcaacgttgt cgccggccgc gtcgagtcga gcgctccgcc
     2341 gccggcgggc gcaggcggga cggccttcaa tgccaagacg atgtggcagt cgaagccgag
     2401 cccagacggc tgggtaacgg tcgaggcggc cttcccgttc gaggtgacgc tgaccggcgt
     2461 cgctgtccac tcgcagcaca gcggccgcta ccacgccgcc gaccgcctgc gggtcgagac
     2521 cccggtggcg cggggcgacc accctctggc tgagttttcg ttgacgtcgg tcgaccagca
     2581 ggtggcgctg tcgccgacca cgacccgcac gtggcgtttc cgcgtccacg ccgccaacgg
     2641 tcgcgtggtc gtcctccggg gccttcgctt cttctcgagc cgtggcgaga ttgtcccgcc
     2701 gctcgttccg tacgggagtt gatgctggaa ggttctgctc tgcgccggtc gggccgtccg
     2761 cgtagagccg agaacgagct atatggcgca tccggcaaga ttcctgacac gtggtgagcc
     2821 aatcggttcc gcagggtccc ggcctggagc gacccggagg tgtcaaccct cggggcggat
     2881 ggaccatatc gttggcgcgg acattctggc gcaggacgat gtcaagatgc ggaaagcttg
     2941 atcccgagat ctgactttgc gagaggccgt ctccatgata gtacctccgt catgcctggc
     3001 gcacgctgct ctggtagagt cgagcttggt tcgggaggtg gatgtccggg cccttgctca
     3061 aggtagacct ttctttcact attcgatgag gaggtcagcc atgaagagcg cgcttcgttt
     3121 catcctcgcg acgatccttt gcggctgtct cgccgcgatg gcgaacgccc agtcgaatgc
     3181 tctcaagtac ggtgacgggc aagcggacgg caagcagagc gtcgggggtt caggcgagat
     3241 gatcgagttc gccctgccga gggagtcacc aaggatcggc ggcctgagaa ttcatggatc
     3301 gcgttacggg atgccggagg cacccgacga gagctttctg atcttcttcc tcagccggga
     3361 tcggaagtct atcgtccata ccgaaatggc gccctattcg cacttcgatc ggggtgccga
     3421 gaaatgggtg acgatcacgt tctcccgccc cgttgaattg ccggaccact tctgggtcgt
     3481 gctcgacttt cgggcgtccc agcgcaaggg agtgtatgtc agttacgaca caagcagcgg
     3541 cggcaaacac tcccggaccg gtctgcccgg caccccggcg ttggagacga agttcggcgg
     3601 agactggatg atcgagctcc ttcccgcgaa gcgagaagcg gtccggtaag gtccgttgac
     3661 aagctttccc gcgccgcgag ttagacatca atacttatgg acggaatgat tatcgcgggc
     3721 cgcttcgagc gccccgacca cacgcccgtg acgggagcag ccgtccacgc cgtcttcctc
     3781 ggcgacccgc ccgccaactg cgggacggca atggacctgc gtcgtacccc tgcacggggt
     3841 cgtcgtcg
//
LOCUS       DATAQL010000644         5447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5410638, whole genome shotgun sequence.
ACCESSION   DATAQL010000644 DATAQL010000000
VERSION     DATAQL010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5410638"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..2440)
                     /locus_tag="VHS97_11250"
     CDS             complement(2..2440)
                     /locus_tag="VHS97_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX3448823.1"
                     /translation="MLSMVMAVSTFAQVGARAVESAGIPPDADSALSIAPSESRLIGR
                     RATVQLIASSRAADGSVQDQTRAVQWLSLNSEVAQPTARGQILPKANGTATIIARLGS
                     LEAHTTVTVVGMDHPAAVSFRRDVIPAFSQAGCNTGACHGTPTGKGGFRLSLRGYLPD
                     QDYGILSRESGGRRINPIAPDTSLILRKPLGEVPHEGGLRLTRNSKSYEFLHDWIMEG
                     AKDDPGATEAISLEILPAARVLNGLATTQQVVARVFDSNGTIRDVTPICYYDSSNPAI
                     AEVDADGYVRFKARGEVAIIAHYLNLVANVRLTHLVDVPGFAEAKVPDDNPIDRAVFA
                     KLNRMRIPPSELCSDREFIRRIYLDVLGVLPTADEVRDFLADSPRARRDDVIDRLLVR
                     PEFYDFWALKFADVLRSNGRLIQTKGAYVFHRWIRECLERNLPLDQLVRELLTSDGST
                     FKNPAANYYRISRDPESAVETTAQLFLGVRIQCAKCHNHPFERWTQDDYYGFAAFFSQ
                     IGRKKGNLPDEEVVYATGSGDVNQPRTGRKMKPKALGGPVLDDQATKDRRVRLATWLS
                     DAKNPFFAKSLVNRIWFHLLGRGIVEPVDDFRDSNPACNDELLEVLTSDFVRNGYDLK
                     KLIRSILQSRTYQLSATANPLNADDSLYFSHAQTRLLPAEVLLDAISTVTATTTAFDG
                     LPRGARATQIPDGKMENPFLKTFGRPARELACECERESDSNLSQALQLIGGATVNGKL
                     RDDSGRMARLAKSGAAPDQVTKELYAVALCRDPSSSELAAATRHLTSSKDLRLAIEDL
                     GWVLINSKEFLFRH"
     gene            2897..3346
                     /locus_tag="VHS97_11255"
     CDS             2897..3346
                     /locus_tag="VHS97_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448824.1"
                     /translation="MFLRLDKGVVRYGLPGLLLGLGMAWISGIRGPEAAAQTGSGGSG
                     VVNSGSARSPEGTRAPSSRPVAGGDVNGTLALITTAGPSQWLYLVDTKDRAFAIYRID
                     PLNPKGTVKLEASRQYKWDLKLEHYNNQAPEPDAIKATVETLARSPS"
     gene            3445..4995
                     /locus_tag="VHS97_11260"
     CDS             3445..4995
                     /locus_tag="VHS97_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3448825.1"
                     /translation="MAQFYTLEEAARVLGMSAEDLKSKAQSREVRAFLDGGSWRFRVV
                     DVDELARRRGLGSDAELRLSDLEMPAAQSGSREVQDLDLSEFHLGVAKPDLGAESAQL
                     SRVKDSGGGKHKDDSDHDILLDDLALPPNQISGSSSVIIGAPSPGGHPSDSDVRLVPD
                     TLKGSSDSDVQLASPTLKQASDSDVTLIKEDTADHGIVGAGSSDTAVRVSPFKGSSAE
                     VPAASSADSDFELNPSSELIDALQPDSGSDFELSALDASDEFESTPLSKPSDSDVTAA
                     DPKLSGINLSRPSDSGINLQTGAGLGLGQQADSIELAPLSDESLQTSKAPPARPSKAK
                     PSLAATPPPAVKKGEKDIFDDTDFEVDVPSSDADSDDKTVQLEAASDFDLEDSDTGSE
                     VFAIDEEAVDQNASTAMAPSAFAEDDEDEEDDGFDSAVSSEMSAGWSSAESSAGQERS
                     APAMVLSREAETEWSGLWVGMLGLTTLSLFFASFVALDMVRNLAEFQAGGTLGSGLVR
                     MIAGMFGG"
     gene            5159..>5447
                     /locus_tag="VHS97_11265"
     CDS             5159..>5447
                     /locus_tag="VHS97_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448826.1"
                     /translation="MVRALSAVPRDFCTQHDRTVRGAKLPVIVALVIGLFLACSGCGI
                     VPKSRMDECQRLTQTLRSENARLKDRVLALQGQNRDYAERAVDDARRLATAD"
BASE COUNT         1089 a         1600 c         1709 g         1049 t
ORIGIN      
        1 ctcaatggcg gaacaggaat tccttggagt tgatcagcac ccagccaagg tcctcgatgg
       61 ccagccggag atctttcgaa gaggtcaagt gcctggtggc cgccgcgagc tcggacgagc
      121 tcggatcccg gcaaagggcc acggcataga gctctttcgt gacctgatcg ggcgctgcgc
      181 cgctcttggc caggcgagcc attcggccgc tatcgtcccg gagcttgccg ttgacagtcg
      241 ccccgccgat caactgcagg gcctgcgaaa gattcgaatc gctttcgcgc tcgcactcgc
      301 aagccagctc acgggcaggg cggccgaacg tcttcaagaa agggttctcc atcttgccgt
      361 cggggatctg ggtggcgcga gcgccgcggg gaagtccgtc gaaggctgtc gtcgtggcgg
      421 tgaccgtcga gatagcgtcg agcaagactt ccgccggcaa cagcctcgtc tgggcatggg
      481 agaagtagag gctgtcgtcc gcgttcagcg gattggctgt cgcactcaac tgataggtgc
      541 ggctctgtag gatcgaccgg atcagctttt tgaggtcata gccatttctg acgaaatccg
      601 acgtgagaac ctcgagcagc tcatcgttgc aggcggggtt tgagtcgcgg aagtcgtcga
      661 ctggttcgac aatgccccgg ccgagcaggt gaaaccaaat gcggttgacc aaactcttgg
      721 cgaagaacgg attcttggca tccgacagcc aggtcgccag gcggacccgc cgatccttgg
      781 tagcctggtc gtcgaggacc ggtccgccca gggcctttgg cttcatcttg cgaccggttc
      841 gaggctggtt cacgtcgccc gacccggtgg catagaccac ctcttcgtcg ggcaaattgc
      901 ctttcttgcg accgatctgc gagaagaacg cagcgaatcc gtaatagtcg tcctgcgtcc
      961 aacgctcgaa cgggtggtta tggcatttgg cacactgaat acgcaccccg agaaaaagct
     1021 gggccgtggt ctcgacggcc gattcggggt cgcggctgat tcggtagtaa ttggccgccg
     1081 gattcttgaa cgttgatccg tcggacgtca ggagctcgcg gaccaactgg tccaatggca
     1141 ggttgcgctc caggcattcg cgaatccaac gatggaagac ataagcgcct ttggtctgga
     1201 tcaagcgacc gttcgagcgg agcacatcgg caaatttcag agcccagaaa tcgtaaaatt
     1261 cgggacgaac cagcagccgg tcaatgacgt catcgcggcg cgccctggga gaatccgcca
     1321 ggaagtcccg gacttcatcc gcagtgggca gcacccccag gacatcgagg taaattcgcc
     1381 tgatgaactc gcggtcgctg caaagctctg agggagggat tcgcatacga ttgagcttcg
     1441 cgaacaccgc ccggtcgatg ggattgtcat cgggaacttt cgcctcagca aaacccggaa
     1501 catcgaccag atgagtcaat cggacattgg cgaccaggtt gagataatgc gcaatgatgg
     1561 cgacctcgcc ccgggccttg aagcgaacgt aaccatcagc gtctacttcg gcgatcgctg
     1621 ggttggacga gtcgtaatag caaattggcg tcacgtcgcg aatggtcccg ttggagtcga
     1681 agacgcgcgc aacgacttgc tgcgtcgtgg ccagtccatt gagcacgcga gccgcgggca
     1741 aaatttccaa agaaatcgct tcggtcgcgc cggggtcatc cttggcccct tccatgatcc
     1801 agtcgtggag gaattcatag ctcttggagt tgcgcgtcag acgtaagccc ccctcgtgcg
     1861 gtacctcgcc cagcggtttg cgtaagatga gactggtgtc cggagcgatg ggatttatgc
     1921 gccggccgcc ggactcgcgc gaaaggattc cgtaatcctg gtccgggaga taaccgcgca
     1981 ggctgagacg aaaccccccc tttcccgtgg gcgtcccgtg gcaggcgccc gtgttgcagc
     2041 ccgcctgact gaacgccgga atcacatcgc gccgaaagct gaccgccgca ggatggtcca
     2101 tcccaactac ggtgacggtg gtatgcgcct cgaggctgcc gaggcgcgca atgatcgtcg
     2161 cagtcccgtt tgccttgggg agaatctgtc ccctcgctgt aggctgggcg acttcggagt
     2221 tcaggctgag ccactgcacc gcgcgtgtct ggtcttggac ggatccgtcg gcggcacgag
     2281 acgacgcgat caattgaacg gtcgcgcggc ggcctatcag tcgcgactcg gacggtgcaa
     2341 tcgacaatgc ggagtcggcg tcggggggta tgccagcgct ttcaaccgcg cgtgccccaa
     2401 cctgcgcaaa agtcgagact gccatcacca ttgatagcat ggatactgcg aagctgcatg
     2461 gagtaaaacg ggagtgcatc aaacgattct ccggcctcgg gtgggacggg cgggcggtgc
     2521 cgtcgggtct tgcggcccag tcgaccttcg cgtgcatgat gtaaggatat cgcaacattg
     2581 acggaatttt caagaaaaat caaacatttt tgactgggac gccttcgtgt actaagcgct
     2641 gcacttgcgt aaagggggat gggctgtaca attgccatgt gttcgagcgc atttcacggc
     2701 cctgtcgtag gtgattctca atcccaagat ctatcgcaca tttcgtccaa atcgtgtggt
     2761 agactgaaga cgatcgtttg gttcccgata ttggatgcgc ccggaatgag ggcgtgacaa
     2821 tgattcgaac atacgacgtt tcagactcgc ctcgggtcta aacgaggaca attgagtctc
     2881 acggaggaga ttgctaatgt ttctacgtct ggacaaggga gtggtgcggt atggcttgcc
     2941 gggcttgctg ctggggctcg gtatggcctg gatttcgggg attcgcgggc cggaggctgc
     3001 ggcacaaacc gggtccggcg ggtcgggagt ggtgaattcg gggtcggctc gatcccctga
     3061 gggaacgcgg gctccgtcgt ctcgcccggt cgcggggggt gacgtgaacg gaacactcgc
     3121 attgatcaca acggccggac cttctcaatg gctgtatctc gtcgacacca aagaccgtgc
     3181 gtttgcgatt taccgcatcg atcccctcaa tcccaaggga acggtcaagc tcgaggcttc
     3241 acgccagtac aagtgggatc tcaagctgga acattacaac aatcaggcgc cagagcccga
     3301 cgcgatcaag gcgacggtag aaactctggc tcgttcgcct tcataaacgg agaccgaaga
     3361 cgtgacgggt cgcggtctcc tttccacgag agttgactag atacggccgc accaaggctg
     3421 atctcgaccg cgaggagcac gtccatggct cagttttaca cgcttgaaga ggccgcccgc
     3481 gtcctgggga tgagcgccga ggatttgaag agcaaggctc agtcgcgcga ggtgcgcgcc
     3541 ttccttgacg gtggatcgtg gcgattccgc gtcgttgacg tcgatgagct ggcgcgacgg
     3601 cgaggattgg gcagcgacgc cgagcttcgg ctgtccgacc ttgagatgcc cgccgcccag
     3661 agcggctccc gcgaggttca ggatctggac ctctctgaat tccacctcgg ggtggcgaaa
     3721 ccggacctgg gggcggaatc cgcacaactt tcgcgggtaa aggattccgg cggaggcaag
     3781 cacaaggatg actccgacca cgacatcctg ctcgacgacc tggcactgcc ccccaaccag
     3841 atctctggct cgagctcggt gatcatcggc gccccctccc cgggcgggca ccccagcgat
     3901 tccgacgtgc gactggtgcc tgacacgctg aagggttcca gcgactccga tgttcagctc
     3961 gcctcaccga ctctcaagca ggctagtgat tcggatgtca ccttgatcaa agaagacacc
     4021 gccgatcacg gtatcgtcgg tgctggatcg agcgatacgg cggttcgagt cagtccattc
     4081 aaggggtcgt cggcggaagt acccgcggcc tcgtcggcgg acagcgattt cgagctcaat
     4141 ccctccagcg agctgatcga cgccttgcaa cctgactcgg gaagtgattt cgagttaagc
     4201 gcgctcgacg cgagcgacga attcgagtcg acaccgctct cgaagccaag tgactccgat
     4261 gtcaccgccg ccgatcccaa gctctcggga attaacctct cgcggcccag cgattcgggg
     4321 atcaacctgc agaccggcgc cggattgggc ttgggacaac aggccgattc gatcgagctg
     4381 gctcctctct ccgacgagtc tctccagacc agcaaggcgc ctccggcacg accgtcaaaa
     4441 gcaaagccat cgctcgcggc gacgcccccg ccggctgtca agaaaggcga gaaggacatc
     4501 ttcgacgaca ctgactttga agtcgatgtc ccgagcagcg acgccgattc cgacgacaag
     4561 accgtccagc tcgaagccgc aagcgatttc gacctggaag atagtgatac cggctccgag
     4621 gtgtttgcca tcgacgaaga ggccgtcgat cagaatgcct cgacggccat ggccccctcc
     4681 gcatttgccg aggacgacga agacgaagaa gacgatggct ttgactccgc cgtttcgagc
     4741 gaaatgagcg ccggctggtc aagcgccgag tcttcggccg gtcaggaacg ctccgcgccg
     4801 gccatggtgc tttcccgcga ggccgaaaca gagtggagcg gtctctgggt cggcatgctg
     4861 gggctcacga cactgtcgct ctttttcgct tcattcgtcg ctttggacat ggtacgaaat
     4921 ctcgctgaat tccaggcggg cgggaccctg ggctcgggct tggtccgtat gatcgccggc
     4981 atgttcggag gctgatcggg tttacaaccg gctacagggc ggagagcgaa ggtcatcccc
     5041 cgatgaacac tcgcatctcc gtctagccgg cccgccgctc gcactgaacg cgtatgcggg
     5101 tcgactatga aagccgatcc ggtcggcgaa cgatcgttcc tgaaaggagt cggaacgaat
     5161 ggttcgtgcg ctttcggccg ttccccgcga tttctgcacg cagcacgacc ggaccgttcg
     5221 gggcgcgaaa ctacctgtca ttgtcgcgct cgtgatcggt ctctttctgg catgcagcgg
     5281 ttgcgggatt gtgcccaaat cgcgaatgga cgagtgccag cggctgacgc agacactgcg
     5341 ctcggaaaac gcccgactga aagaccgagt tttggcgttg caaggccaga atcgagacta
     5401 tgccgagcgc gccgtcgacg atgcgcgtcg gctagcgacc gcggacg
//
LOCUS       DATAQL010000645         5706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4494045, whole genome shotgun sequence.
ACCESSION   DATAQL010000645 DATAQL010000000
VERSION     DATAQL010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4494045"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..979
                     /locus_tag="VHS97_11270"
     CDS             257..979
                     /locus_tag="VHS97_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX3448827.1"
                     /translation="MATSVISKQRDSACDGVPAHRFPVWKAAVVAFVVCALSLTWVFR
                     RPWFEGNLGVVDVGRVIRSAQPTSQLPRLVAEYHLKSILNLRGGSPADPWYNAEVRTA
                     QASDLTYYDLPLSATRRPTRRELLFLIDILERCPYPLLIHCKSGADRTGLASALYLMT
                     HKGIPPEQAEGSFALEFGHIPVAGTQHLHEPLREYADWLKARQLPHTAERFRAWVKND
                     YRSSDPSIDPPLWPTGPRPRRT"
     gene            complement(1040..3442)
                     /locus_tag="VHS97_11275"
     CDS             complement(1040..3442)
                     /locus_tag="VHS97_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEX3448828.1"
                     /translation="MAKLRWLAVLAFTLFAADSARGALPDANPVSLGFDAERLKRIDA
                     AIDQAINRKEVPGAVVLVGRRGMIAYARVTGQRALEPKPEPMTRDTIFDMASLTKPIV
                     TATSIMILIDEGKLRLEDRIVVSLPDFDNNGKGEITIEQLLRHRAGFVPDNPMSDYEH
                     GTAAAWQRIAQLELVSHPGDKFKYSDVGFLVLGKLVERAAGRGLDVFAREKIFEVLGM
                     NDTYFRPLESSRLAPAAVERVAPTERETPGGRMLRGVVHDPRSRALGGVAGHAGLFST
                     ADDVAIFAQTLLNGGVGPNGRRVLSPLAVRAMTDTGTTPPGQRRGLGWDIETSYSSPR
                     GSLYGSSSFGHTGFTGTSLWIDPETESFVVILTSRLHPDGHVPAPTSLRKTIATLAAA
                     AIVDSSSRSTGASLRSASSSNASTATTLLANDRHTVQCGIDVLVAEQFRPLNGLKVGL
                     VTNHTGRAKNGQSTIDVLFHAPGVTLVKLFSPEHGIRGQVDAAVADGKDDSTGLPIIS
                     LYGKDRKPRASDLEGLDALVYDIQDIGARFYTYITTLGLVLEAAKENGKKVFVLDRPN
                     PIGGQTVSGPVRDPEFESFIAYHPLPVRHGLTVGELALLYNAERKIGASVEVVKCRGW
                     SRDDLYDRTGLIWINPSPNMRSLTEALLYPGVGLLEATNLATGRGTDTPFERVGAPWI
                     DPAPFAAALNSAAVPGARFVPIYFTPTERQHAGRRCGGVQILVTSWSEFDPLRLGMTL
                     ATRLRTVYPNDWQPDGLLRLLCDRATYDRVLAAKPVEAIMAGWIDELATFQKIRARYL
                     LY"
     gene            3737..5497
                     /gene="aspS"
                     /locus_tag="VHS97_11280"
     CDS             3737..5497
                     /gene="aspS"
                     /locus_tag="VHS97_11280"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX3448829.1"
                     /translation="MLYKRTHSCGELSVDDVGKPVILNGWVDAYRDFGGLVFIDLRDR
                     YGITQVVFEPDAGKELQAKAGELRNEYVVGIKGTVAPRLAGKENPRLKTGGIEVRARE
                     LVVYNATPTPPFEMHGPEPNEELRLTYRYLDLRRPAMQEIFILRHRLTQVMRSTMSAL
                     GFLEIETPILGRSTPEGARDFLVPSRVHTGHFYALPQSPQIYKQLLMVSGFDRYFQIA
                     RCFRDEDLRANRQPEFTQLDVEMSFVDDHDVTSTMEILVAAIAKDFGGEAPKLPLPRL
                     DYHDVMERFGNDRPDLRYGLELKDVAHIAEQTEFKVFQHAKGAGHRIRGLCVPGGAGH
                     YSRKDLDGLTEWIAGMGAKGLIWLKVENDTLTGPTAKFFAPEVQHQLRTHFEARSGDL
                     ILIVADTQAVSSMALSNLRVRVAAELKLFDPKSFHYSWVVRFPLFAWDAEEKRYVAEH
                     HPFTMPLFEDLHLLDSEPAKVRAQAYDLVVNGEECGGGTIRVHDPVIQSKIFALLGLT
                     PEEAQEKFGFLLSALRNGAPPHGGIALGVDRLVMLYSGLTNIRDCIAFPKTAKGTDLM
                     TGAPGTVDARQLKELHIKPH"
BASE COUNT         1120 a         1716 c         1736 g         1134 t
ORIGIN      
        1 ctcttcggac agacctcctc gccccttata tctatctcat gacagtgtcg cgttttcttg
       61 atgggccggt tttccctcgg gcgaaggggg cgaaccggcc catcgtggtt tgaacccata
      121 ggtcgccagc cggcgatcca catgcgcatt tcccttgcgc aatatccgga catccggacc
      181 atgggccgag aaccgcggtg gacgcggaca tcaatttatg gttagctgcc ggctcgcgac
      241 gcaaacggag tattccttgg caacgtccgt gatctcgaaa caacgtgact cggcttgcga
      301 cggcgtacct gcacatcgtt ttccggtgtg gaaggcggcg gtcgtcgcgt tcgtcgtttg
      361 cgcactcagc ttaacttggg tctttcgacg tccatggttt gaaggcaatc tcggcgtggt
      421 cgacgttgga cgggtgatcc gctcggctca gccgacttcg caactgccac gcctggtagc
      481 ggaataccat ctcaagtcga ttctcaactt gcggggcggt agtcccgcgg acccgtggta
      541 taacgccgag gtacggacgg cccaggcgag tgatctgacc tattacgacc ttccattgag
      601 cgcgactcgc cgaccgactc gtcgcgagct gctcttcttg atcgacatcc tggagcgatg
      661 tccctacccc ttgttgatcc actgcaagtc gggcgctgac cgaactgggc tggcatcggc
      721 gctttatctc atgacgcaca aaggtatacc gccggagcag gcggagggat ccttcgcact
      781 ggagtttggc cacattcctg tcgccggaac tcaacatctg cacgagccgc tgcgcgagta
      841 cgccgactgg ctcaaggccc ggcagctccc gcatactgcg gaacggtttc gcgcctgggt
      901 caagaacgac taccgatcca gcgacccatc tattgatcca cctctctggc cgactggccc
      961 gcgaccgcgg cgaacgtgaa gtcgctcgca cggcgtgcct tgtagcggta tcgttttgaa
     1021 agcaccagcg ctcgccggtt caatagagca agtagcgcgc gcggattttt tgaaacgtcg
     1081 cgagctcatc gatccatccg gccatgatcg cctcaaccgg cttggcggca aggaccctgt
     1141 cgtacgttgc ccgatcgcaa agcaggcgga gcaaaccgtc gggttgccag tcgttggggt
     1201 acaccgtccg tagtcgggtg gccagcgtca tgccaagccg gagcgggtca aattcggacc
     1261 agcttgtaac cagaatctgc acgccgccac aacgtcggcc ggcgtgctga cgttccgtgg
     1321 gcgtgaagta gatgggaacg aaccgagcgc cgggaactgc ggcggaattg agcgcagccg
     1381 cgaatggcgc tggatcgatc cagggcgccc cgacccgctc aaacggtgta tccgtgcccc
     1441 ttccggtcgc cagattggtc gcttccagca ggcccactcc ggggtacaag agcgcctcgg
     1501 tcaggctgcg catgttgggg gacgggttga tccatataag cccagtgcga tcgtagagat
     1561 cgtcgcgcga ccagccgcgg cacttgacca cttccaccga ggccccgatc ttgcgctctg
     1621 cgttgtaaag caaggcgagc tcgcccaccg tcaacccatg acgcactggc agtggatggt
     1681 aggcgatgaa cgattcaaac tcgggatcgc gcaccggccc ggagactgtc tgcccaccga
     1741 tcgggttggg tcggtcgagc acaaatacct ttttgccatt ctctttggcc gcttcgagca
     1801 ccaggccgag cgtcgtgatg tacgtgtaaa aacgtgcgcc gatgtcttga atgtcgtaaa
     1861 ccagggcgtc cagaccttcg aggtccgagg cgcgcggctt gcgatccttg ccgtagaggc
     1921 tgatgatcgg caacccggtc gagtcatcct tgccgtcggc caccgcggca tcgacttgtc
     1981 cacggatgcc atgctcgggg ctgaacagtt tgaccagcgt cacacccggg gcatggaaga
     2041 ggacatcgat cgtcgactgg ccgttcttgg cccgaccggt atggttggta accagcccga
     2101 cttttagccc gttcaacggc cggaattgct cggcgactag tacatcgatg ccgcattgca
     2161 cggtatgacg gtcgttcgcc aataatgtcg ttgcggtgga agcgttcgaa ctggaagcgg
     2221 atcgcagcga ggcgccagtc gaccgcgagg atgaatcgac gatcgcggcg gcagcgaggg
     2281 tcgctattgt cttgcgaagc gaagtcgggg cggggacgtg accgtctggg tgcaagcggc
     2341 tggtcaggat gacaacgaaa gactcggttt cggggtcaat ccagagactg gttccggtaa
     2401 aaccggtgtg gccaaaacta ctgctgccgt agagtgagcc tcgcggcgaa ctatagctgg
     2461 tctctatgtc ccagcccagc ccccgccgct ggcctggggg tgtcgtcccg gtgtcggtca
     2521 tggcccgcac ggccagcggc gagaggacac ggcggccgtt tggaccgacg cctccgttca
     2581 atagcgtttg ggcaaagatc gccacatcgt cggcggtcga gaacagacca gcgtgaccag
     2641 cgacacctcc caacgcccga gaacgaggat cgtgtacgac cccgcggagc atccgtcctc
     2701 caggcgtttc ccgctcggtc ggagcgacgc gttcaaccgc cgccggagcc aaacgcgacg
     2761 actccaacgg gcggaagtac gtgtcgttca ttcctagtac ctcaaaaatc ttttccctgg
     2821 caaagacatc caggccacgg ccggcagcgc gttcgaccag tttgccgagg acgaggaacc
     2881 ccacgtcgga gtacttgaat ttgtcgcccg gatgcgagac caactcgagc tgggcaatgc
     2941 gctgccacgc ggcggcagtg ccgtgctcat agtcagacat cgggttatcc ggaacaaagc
     3001 cagcgcgatg gcgaagcagt tgctcgatcg tgatctcgcc cttgccgttg ttgtcgaaat
     3061 cgggaagaga gacgactatt cgatcctcca ggcgtaactt gccctcatcg atcaagatca
     3121 tgatcgaggt cgcggtaacg atgggcttgg tcaacgaggc catgtcgaag atggtgtcgc
     3181 gcgtcatcgg ctcaggcttg ggttcgaggg cccgctgccc ggtcacacga gcgtaagcga
     3241 tcatgccccg ccggcccacc aggacaacag cgcctggcac ttccttgcgg ttaatcgcct
     3301 ggtcgatcgc ggcatcgatc cttttgagcc gttcggcatc aaaccccaat gacaccgggt
     3361 tagcgtccgg tagagccccc cgagccgaat cggctgcgaa caaggtaaaa gcaagtacag
     3421 cgagccatcg tagctttgcc atgaatcaca aaactccaaa cgtcggacgt ggaagcaccg
     3481 ttcgggaact attgttgtcg tggattcgat ggtgccgacg cgcatcggcc ggtgcaatcg
     3541 tcaagtcccg ggagcgtgtg attcggtcac ttcccatgcg tcgccaatgg cggtcgccga
     3601 gttcgtcgcg accgcggcga actcggcaga cgggttgtgt gtcccaatct ccgacggctt
     3661 gacggtttcc ggctagaagg ggtatcgtgg ccaccgaagg aattggctcg aatcatcttg
     3721 tcgctcgatg tgaattatgc tttacaaaag aacgcattcc tgcggtgaat tgagcgtgga
     3781 tgacgtgggt aaaccggtca tcctcaatgg ctgggtcgat gcctatcgcg acttcggcgg
     3841 cctggttttc atcgaccttc gcgaccgtta cgggatcact caggtggtct tcgagcccga
     3901 tgcgggcaaa gaactccagg ccaaggccgg cgagctgaga aacgaatacg tcgtcgggat
     3961 caagggaacg gtcgctccta gactggcggg caaggagaat cccaggctca aaaccggtgg
     4021 gatcgaggtc cgcgcccgtg agctggtggt ttacaacgcc acgccgacgc cacctttcga
     4081 gatgcatggc cctgagccca acgaagagct caggttgacc tatcgctacc tcgacttgcg
     4141 ccggccggca atgcaggaga ttttcatcct ccggcaccgg ctgactcagg tgatgcgcag
     4201 cacgatgagt gctctcggtt tcctcgagat cgagacgcct attctgggcc gtagcacgcc
     4261 cgagggcgca cgcgacttcc tggtcccgag ccgagttcat accggccatt tctacgcgtt
     4321 gccccaatcg ccgcagatct acaagcaact cttgatggtc tcgggattcg atcgctattt
     4381 ccagatcgca cgctgtttcc gtgacgaaga cctacgcgcc aacaggcaac ccgagttcac
     4441 tcagctcgac gtcgaaatgt cgttcgttga cgaccacgat gtgacttcga ccatggaaat
     4501 cttggtggct gcgatcgcca aggattttgg cggcgaagcg cccaaactcc ctttgccgcg
     4561 tctggactat cacgacgtga tggagcggtt cggcaacgat cggcccgact tgcggtacgg
     4621 gctcgagctc aaggatgtcg cccacatcgc cgaacagacg gagttcaaag tctttcagca
     4681 tgccaaggga gctggtcatc ggatccgcgg cctgtgcgtg ccgggtggag cagggcatta
     4741 cagccgcaag gatctggacg gtctcaccga gtggattgcg ggcatgggtg ccaagggctt
     4801 gatatggctc aaagtcgaaa acgacacgtt gacaggcccg accgccaagt tctttgcgcc
     4861 tgaggtccag caccagctca gaactcattt tgaagcccga tccggcgacc tgatcttgat
     4921 cgtcgccgac acccaggcgg tcagcagtat ggcactttcc aacctgcggg tcagggtagc
     4981 tgccgagctc aagctgtttg accccaaatc gttccattat tcgtgggtcg tccggtttcc
     5041 gcttttcgcc tgggatgccg aagagaagcg atacgttgcc gagcatcacc cgttcacgat
     5101 gccgctcttc gaagacttgc acctgcttga ttccgagccg gccaaggtgc gcgcccaggc
     5161 ctatgatctg gtcgtcaacg gcgaggagtg cgggggcggg accatacgcg tgcacgaccc
     5221 ggtgatccag tccaagatct ttgcccttct gggcctcacg cccgaagaag ctcaagagaa
     5281 gttcgggttc ctactgagtg cgctgcgcaa cggcgctcca ccgcatggcg ggatcgcgct
     5341 gggagtcgac cggctcgtta tgctttattc ggggctgaca aacattcgcg attgcatcgc
     5401 cttccccaag acggccaagg gaactgacct catgaccggc gccccgggga ccgtcgatgc
     5461 ccgacagctc aaagaactgc acatcaagcc gcattgagat tcggcaatag cgacttggcc
     5521 tttagaacgg tgcaattcga tgaacaacgg ttccctgatt ttcgccggcg ttcgcacgcc
     5581 gatgggagcc atgaatggat ccctggccag cgtctcggcc cctgaactgg gagcggcctg
     5641 tatcagggct ctggtgtctc gaaccggggt tgccggcgac gatattgacg aagtgatcat
     5701 gggcaa
//
LOCUS       DATAQL010000646         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5257649, whole genome shotgun sequence.
ACCESSION   DATAQL010000646 DATAQL010000000
VERSION     DATAQL010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5257649"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2082)
                     /locus_tag="VHS97_11285"
     CDS             complement(<1..2082)
                     /locus_tag="VHS97_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX3448830.1"
                     /translation="MVVLLRAAMLSFVALAVNSVCLRIAGGLTVALMLFASVLTEQRA
                     IHGLMFTYALLGSYWLILLYWSQIQVKLLEGRSKRPPILAIAVWGLFIGLLIALVVGP
                     RQTIGVLAELLGTSGGTTTASADARGGVGDGQDLVSGTDNARSTGPVESDIFLETHDK
                     SLYDAANEKWGDPEKPKKREQSLAVAVTNQAESFREEKSKQPNAAREFSVVRKRRRDP
                     LTPPTVEADAAYFVKGKTPLHIRLAAFDEFDGRRWIEPALQHQSDGLGLGFGGWMILP
                     ALPSSVVAGYLSHRIVVGNIDAPQIPIPPYAHSFRIDLVDRPDFFRFSQPGILIYRIN
                     TGLPRQTVLDSRSHTVDPEGLRDLRFPAHCTYAHPKLLSLGRAGTRSGNESGHNTGTS
                     FDPRILDLASSWARGAERGWRQVEAVVIRLREGFQYDPEAGVPVDAPDAMSYFLFEQR
                     RGDDYAFATAACLLLRSLGYPCRFVEGLYADPGRYDPKTQQTAIKMPADLHTWAEVML
                     PSHDWIVVEPTPGFDVLGPEQGILARLGGLATKLIGILRHHPRWGIAFVVVFLTLIGA
                     WRSVGMACANLAWWFEFRGTLRAQVLATVRLLERRARLGGRSRPLGMTLRVWYRAVAT
                     PQTDEASRRALDSFLGIAGWALYGSPDERLTPGMRDDQVESHCREFVRMWPAKRLALT
                     ATKAERRTRSVA"
BASE COUNT          411 a          678 c          666 g          366 t
ORIGIN      
        1 cgccacagat cttgttcgcc gttcggcctt cgtcgccgtt agcgccaggc gctttgccgg
       61 ccacatgcgc acgaattccc ggcagtgact ctcgacctgg tcgtctctca tgcccggagt
      121 taaccgctcg tccggcgatc cgtagagggc ccaccccgcg atcccaagga aactatcgag
      181 cgctcggcgc gacgcctcgt cagtctgcgg ggtagcgacc gcgcgatacc agacgcgcag
      241 ggtcataccc agcgggcggc tgcgaccgcc cagtctggca cgccgctcga gaagccgcac
      301 tgtcgccagg acctgagcgc gcagcgttcc gcggaactcg aaccaccatg ccaaattcgc
      361 acaagccatg ccaaccgagc gccatgcccc aatcaaagtg aggaagacca cgacgaacgc
      421 gatcccccat cgtggatggt gccgcagaat cccgatcaat ttggtcgcca gaccgcccag
      481 acgtgcgaga atgccttgct ccggaccgag gacatcaaag ccaggagtcg gctcgaccac
      541 gatccaatcg tggctcggca acatgacctc ggcccaggtg tgcaagtcgg cgggcatctt
      601 gattgccgtc tgctgagtct tggggtcata ccgcccggga tcggcataga ggccctcgac
      661 gaagcggcat ggatatccca ggctccggag taacaggcaa gcggccgtgg cgaatgcgta
      721 atcgtcaccg cggcgttgct cgaacaggaa gtaggacatg gcgtccggcg catcaacagg
      781 tactccagct tccggatcgt actgaaaccc ttcgcgcaac cgaatcacga cggcttcaac
      841 ctggcgccag ccgcgttccg cgcctcgtgc ccaggatgaa gccagatcga ggatccgtgg
      901 atcgaacgac gtcccggtgt tgtgccccga ttcattgccc gagcgcgtgc cggctcggcc
      961 aaggctgagc aacttcgggt gcgcgtaggt gcagtgcgcg gggaatcgca ggtcccgcag
     1021 cccttccggg tcgaccgtgt gcgatcggga gtcaagtacg gtctggcgcg gcaggccggt
     1081 gttgatccgg tagatgagga tgcccggctg tgaaaaccgg aagaagtcgg gccggtcgac
     1141 aagatcaatc cggaagctgt gcgcgtatgg tggaatcggg atctggggag catcgatgtt
     1201 ccccacgacg atcctgtgcg acaggtaacc cgccacgacc gaggacggga gagccggtaa
     1261 gatcatccac ccgccgaatc cgaggccaag accgtcggac tggtgctgga gagccggctc
     1321 gatccagcgc cgcccatcga attcgtcgaa ggcggcgagg cgaatgtgca gcggcgtctt
     1381 ccccttcaca aagtaagcgg catcggcctc tacggtcggt ggcgtcaacg ggtcgcgccg
     1441 tcgcttccgg acgaccgaga attcacgggc tgcgtttggc tgtttcgact tttcttcccg
     1501 gaatgactcc gcctggttcg tcaccgcgac cgccagcgat tgctcacgtt tcttgggctt
     1561 ttcgggatct ccccatttct cgttggcggc atcgtacagg gacttgtcgt gagtctcgag
     1621 gaagatgtcg ctctcgacgg ggcccgtgct ccgcgcgttg tccgtaccgc tgacgagatc
     1681 ctggccgtct cccacgcccc cgcgagcgtc ggcggaggcg gtcgtggtcc cgcccgacgt
     1741 gccgagcaat tccgccagaa caccaattgt ctggcgcggg ccaacgacaa gggcgatcaa
     1801 caatccgatg aagaggcccc aaaccgcgat cgccaggata ggcggtcgtt tgctccttcc
     1861 ctcgaggagt ttcacctgga tctggctcca gtacagcaag attagccagt agctgcccag
     1921 taacgcataa gtgaacatga ggccgtggat cgctcgttgc tcggtcagca ccgaggcgaa
     1981 gagcatgagg gccacggtca gtccaccggc aatgcggagg cagactgagt tgacggcgag
     2041 agcgacgaac gagagcatcg cagcgcggag cagtacgacc atcagcagct ccgtcgccaa
     2101 tcgctctgtc gttgcaaacg c
//
LOCUS       DATAQL010000647         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3730998, whole genome shotgun sequence.
ACCESSION   DATAQL010000647 DATAQL010000000
VERSION     DATAQL010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3730998"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..717
                     /locus_tag="VHS97_11290"
     CDS             106..717
                     /locus_tag="VHS97_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448831.1"
                     /translation="MRTLFTAATLVAAWGIGAPTVVAQSKKIDLQTKAGVAAVKGEWK
                     YHDVKIVEVEGKGPDGKPNKTYNVEPRAHASDFDDTKWEVVAPETLKDPRSTGQVCFC
                     WYRTKITIPAEAAGKAVFFQTTVDDYGEVWVDGKLPRTPGKGGEAVVAGFNAPNRVEL
                     KDPQPGKVYQIAVFAINGPISAAPSNWLFLKNTALEIVDKPSQ"
     gene            760..1653
                     /locus_tag="VHS97_11295"
     CDS             760..1653
                     /locus_tag="VHS97_11295"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX3448832.1"
                     /translation="MTLLETNLGSGSIPKRQGKVRDVYDLGDRLLLIATDRISAFDWV
                     MPNGIPDKGRVLTCLSADWFERLLVPNHLISTEINDAGLDLEPEEQQSLTGRTMVVRK
                     ARVVPFECVVRGYLAGSGWKEYRESGTVCGITLRPGLVQSDRIDPIFTPATKAETGHD
                     ENVSFETMASAVGREVTNTLQSMSLEVYRVAAKHALDRGLILADTKFEWGFDNETGEL
                     LLVDEVLTPDSSRYWSLETYSPGGPQPSFDKQFVRDWLETTGWDKSSPPPRLPEDVVA
                     RTRQKYVEAFERLTGHAFPWR"
     gene            1679..>2626
                     /gene="aroC"
                     /locus_tag="VHS97_11300"
     CDS             1679..>2626
                     /gene="aroC"
                     /locus_tag="VHS97_11300"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX3448833.1"
                     /translation="MLRYLTAGESHGPALTAIVEGFPSGIKLDTALIDRELERRQGGY
                     GRGKRQTLETDRVIVESGVFHGVTTGAPITLRLINNDAKLERLAEPASPRGGHIDLAG
                     AINYQTGIRQVLERASARETALRVAVGALAKLLLQELGISVFGYVLELGGILAAPQSR
                     DVAVRDASPVYTLAPEVDAAMIAAIDAARAAGDTLGGVVETVVTGCPIGLGSHAQWDR
                     KLDARLGAAVLSVQAIKAVEIGLGVEAARRPGSKVMDPIAYDPDSPADDRRHGFRRPT
                     NNAGGIEGGTSNGEPIVVRAAKKPISTLAARGPSVNMATK"
BASE COUNT          520 a          786 c          852 g          468 t
ORIGIN      
        1 aactgatttc gtcctcttac gttacccccg cttgatttga ttgtattgcg gccaaaccta
       61 gctgctttga cctatgtgtc tcgtaggcca gtggagagcc tgttaatgcg gacgttgttt
      121 actgcggcga ctttggtcgc ggcctggggt attggtgcac cgaccgtggt cgcgcaatcg
      181 aaaaagatcg acctgcaaac caaggcgggg gtggctgccg tcaaggggga atggaagtat
      241 cacgacgtga aaatcgtcga ggtcgagggc aaggggcccg acggcaaacc gaacaagacc
      301 tacaatgtcg aacccagggc tcacgcatcc gattttgacg acaccaagtg ggaggtggtc
      361 gcccccgaga ctctcaagga tcctcgatcg accggccaag tctgcttctg ctggtatcgg
      421 accaagatca cgattcccgc cgaagcggct ggcaaggctg tctttttcca gacgaccgtc
      481 gacgattacg gcgaagtctg ggtcgacggc aagctgccgc ggacgccggg caaagggggc
      541 gaggctgtcg tcgctggctt caatgcacct aatcgggttg agctcaagga cccgcagccg
      601 ggaaaggtct atcagatcgc cgtgttcgcg atcaacggac cgatctcggc agcaccgtcc
      661 aactggctgt tcctcaagaa cactgccctc gaaatcgtgg acaaaccgtc ccagtgaata
      721 agcggacgcg cgtaatcgtc gagtcaggga aaaacggcga tgacattgct cgagacaaac
      781 ctgggttcag gttcgatccc caagcgccag ggcaaggtgc gggacgttta cgatctgggt
      841 gatcggctgc tcctgatcgc gaccgaccgc atcagcgcat tcgattgggt catgcccaac
      901 gggattccgg acaagggacg cgtgttgaca tgcctgagcg ccgattggtt cgaacgcctg
      961 ctggtaccca atcaccttat ttcgaccgaa atcaatgacg cagggctcga tctcgagccg
     1021 gaagagcaac aatcgctcac gggccgcacg atggtggtac gcaaggcgcg cgtcgttccg
     1081 tttgagtgcg ttgtgcgggg ttatctcgcg gggagcggat ggaaggaata tcgcgagtcg
     1141 ggaacggtct gtggtattac ccttcggccc ggcttggtcc aaagcgatcg aatcgacccc
     1201 attttcaccc ccgccaccaa ggccgaaacc ggtcacgatg agaacgtgtc gttcgaaacg
     1261 atggcgagcg ccgtcggcag agaagtgacg aacacgctac aatcgatgag cctggaagtc
     1321 tatcgagtcg cggcgaaaca cgccctcgat cgggggctga tcctggccga caccaagttt
     1381 gaatgggggt tcgacaacga gaccggcgag ctgctcttgg ttgacgaggt gctgacgccg
     1441 gacagctcgc ggtactggtc gctggagacg tattctcccg ggggtccgca gccgtcgttt
     1501 gacaagcagt tcgtacgcga ctggctcgaa accacgggct gggacaagtc gagcccgccc
     1561 cctcgtttgc cggaggacgt cgtcgccagg acccggcaaa agtatgtcga ggcgttcgag
     1621 aggctgaccg ggcacgcgtt tccttggcga tgacagctct ctacaggcgc gccatgtcat
     1681 gctgcgatat ctgaccgccg gcgagtcgca tggaccggca ctcacggcca tcgtggaggg
     1741 gtttccgtcg ggcatcaagc tcgataccgc gctcatcgac cgcgagctcg agcggcgcca
     1801 ggggggatat ggtcgcggca agcggcagac gctggagacc gatcgggtca tcgtcgagtc
     1861 gggcgtcttt cacggcgtaa ccacaggcgc accgatcaca ctccgcctga tcaacaacga
     1921 cgccaagctc gagcgtcttg ccgagccggc ctccccgcgc ggggggcaca tcgacctggc
     1981 cggcgccatt aactaccaga ccgggattcg ccaggtgctc gagcgcgcca gcgcgcgaga
     2041 gacagcgctg cgggtggccg ttggagcact cgccaagctg ctgctacaag agctgggaat
     2101 atccgtgttt ggttacgtcc tcgagctggg gggcatcctt gccgcaccgc aatcgcggga
     2161 tgtggccgtt cgcgacgcca gcccggtgta cacgctcgca ccggaggttg atgccgcgat
     2221 gattgccgcg atcgacgccg cccgagcggc cggcgacacg ctggggggcg tggtcgaaac
     2281 ggtcgtcacc ggctgtccga tcgggctagg tagtcacgcc cagtgggatc gcaagctcga
     2341 tgcccgcctt ggcgccgccg tgttgagcgt gcaggcgatc aaggctgtcg agatcggcct
     2401 tggtgtcgag gccgcccgtc ggcctggatc caaggtgatg gacccgatcg cgtatgaccc
     2461 tgactcgccg gccgacgacc gccgccatgg atttcgccga ccgaccaaca acgctggtgg
     2521 aatcgaagga ggcacctcca acggcgagcc gatcgtggtg cgcgccgcca aaaagccgat
     2581 cagcaccttg gcggcgcgcg ggccatcggt caacatggcc accaag
//
LOCUS       DATAQL010000648         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4646530, whole genome shotgun sequence.
ACCESSION   DATAQL010000648 DATAQL010000000
VERSION     DATAQL010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4646530"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VHS97_11305"
     CDS             <1..603
                     /locus_tag="VHS97_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3448834.1"
                     /translation="RESQRLRRWAPKSQWPANAISVGIMGLGVLGANAAGVLTRIGFR
                     VAGWSRSERRIEGVTCFHGETGFEAFLRQSDILVCLLPLTADTRHILNRGVFAKLNRS
                     SPMGAPVVINAGRGGLQNEAELLACLDDGTLGAASLDVFAEEPLPPDSPFWSHPKVVL
                     TPHNAADTDPDEISKYVARQIERFEAGGALDNVVDRARGY"
     gene            complement(637..>1536)
                     /locus_tag="VHS97_11310"
     CDS             complement(637..>1536)
                     /locus_tag="VHS97_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEX3448835.1"
                     /translation="ICRHIPWRRPIGSLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNK
                     KAEVIRVYLPPDANCLLSVTDHCLRSRNYVNVVVAGKQPAPQWLTMDAAIKHCEAGLG
                     IWEWASNDRGGEPDVVMACCGDVPTLETLAAVDLLRRYAPDLKVRVINVVNLMKLQPP
                     SEHPHGLSDQDFDALFTTDRPIIFAFHGYPWLIHRLTYRRAGHPNLHVRGYKEEGTTT
                     TPFDMCVLNDLDRFHLVADVIDRVPGLGSRAAYAKQAIRDKLIDHRAFIARYGDDMPE
                     ITGWRWGQEAGSAAVRSTESDNV"
BASE COUNT          284 a          454 c          505 g          293 t
ORIGIN      
        1 cgggaatcgc agcgcctcag gcgttgggca cccaaatcgc agtggccggc gaatgcgatt
       61 tcggtcggca tcatggggct tggtgtgctc ggcgcgaacg ccgcaggggt actgacacgc
      121 atcggctttc gcgtcgccgg ctggagccgg agcgaaaggc ggatcgaggg cgtgacatgc
      181 ttccacggcg agaccggctt cgaagccttt ttgcgccaga gcgatattct ggtgtgcctg
      241 ctgccgctaa cagcggacac ccggcacatc ctcaaccgcg gcgtttttgc aaagctgaac
      301 cgaagcagcc cgatgggcgc gccggtggtg atcaatgccg gccgcggcgg cctgcagaac
      361 gaggccgaac tccttgcatg ccttgacgac ggcacgcttg gcgcggcgtc gctcgacgtc
      421 ttcgccgagg agccgttgcc gccggacagc ccgttctgga gccatcccaa ggtggtgctg
      481 acgccgcaca atgcggccga caccgatccc gacgaaatct ccaaatatgt cgccaggcag
      541 atcgagcgat ttgaagccgg cggcgcgctg gataacgtcg tcgaccgggc acggggctat
      601 taggatgtcg cgccggcgga aggcaggata gtcggctcag acgttgtcgg attctgtgga
      661 acgaactgcc gcggatcccg cctcctgtcc ccatctccag cctgtgatct ccggcatatc
      721 gtccccgtac cgggcaatga acgccctgtg gtcgatgagc ttgtcccgga tcgcctgctt
      781 ggcgtaggca gcccgtgatc ccagtccggg cacgcgatcg atcacatcgg ccacgaggtg
      841 gaagcgatcg agatcgttga gtacgcacat gtcgaacggg gttgtcgtgg tgccttcttc
      901 cttgtagccg cgcacatgca ggttgggatg gcccgcccgc cggtaggtca atcggtgtat
      961 gagccaggga tagccgtgga aggcgaaaat gatcggcctg tcggtcgtga acagcgcgtc
     1021 gaaatcctga tcagagaggc cgtgcgggtg ttcgcttggt ggctgtagct tcatcagatt
     1081 gacaacgttg atgacacgca ctttcaagtc gggcgcatat cttcgaagca ggtcgacggc
     1141 tgcaagcgtc tcgagcgtcg gtacgtcgcc gcagcaagcc atcacaacgt cgggctcacc
     1201 gccccgatcg ttgctggccc actcccagat gccaagcccg gcctcacagt gcttgattgc
     1261 cgcgtccatg gtcagccatt gaggggcggg ctgcttcccg gcgacgacga cattcacgta
     1321 gttccggctg cgcaagcagt gatcagtgac cgagagcagg cagttggcgt cgggagggag
     1381 atagacacgg atgacctcgg ctttcttgtt tacgacgtgg tcaatgaagc cagggtcctg
     1441 gtggctaaag ccgttgtgat cctgtcgcca gacatggctt gacaaaagat agttgagcga
     1501 gccgatgggc ctccgccatg ggatgtgcct acagat
//
LOCUS       DATAQL010000649         6215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_678300, whole genome shotgun sequence.
ACCESSION   DATAQL010000649 DATAQL010000000
VERSION     DATAQL010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678300"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VHS97_11315"
     CDS             complement(<1..196)
                     /locus_tag="VHS97_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448836.1"
                     /translation="MADTDSLYEAVTTHEMYARNRRNGDRARPAPARRAVTTTRNRYH
                     LVLKLIRRGHLFTGLFMTPWI"
     gene            complement(622..1926)
                     /locus_tag="VHS97_11320"
     CDS             complement(622..1926)
                     /locus_tag="VHS97_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3448837.1"
                     /translation="MPQSRWNDRHAATWLVTDGQGRLLTSPTNLPDEELPLSWVSAMG
                     AGTLPDRVTDMKRRRWRAAQVRVAPTRGRPSGVDRPQNTPDGKSYHDEIVLAAFGSLE
                     ETESTLTSLGSFLVCISILVWFTAALCARSLMRKTMEPLTRLVESAQALDAANPGWAL
                     AEVGTRDELDELRRAFNDLLARLHEAYDRQRRFSSEASHQLRTPVAVMIGHLEVAQRY
                     DRSGEQYRHVIHVAHKRAVELGQIVESLLFLSRPDSSTLTKFERLDLNCWLMKYLDSR
                     PKNHRSRDIVLRSDEQGPLWIKAQPNLLGQLVENLLDNACKYSFSGQPVVLATASEDG
                     SALLVVEDLGCGIAPEDLTRIFEPFFRSSSTSPQRVPGVGLGLSVVARIARAFGGTVT
                     AQSEIGRGSRFDVRLPMTEFSDQNSSAIPRPRPELTDAICVP"
     gene            complement(2133..2819)
                     /locus_tag="VHS97_11325"
     CDS             complement(2133..2819)
                     /locus_tag="VHS97_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3448838.1"
                     /translation="MARTILIIEDELEFAEFVMTGLAEEGFSVEHAVDGRQGYELLQS
                     RPWDLVLLDWSLPHQDGLSLLRGFRRSGLEAPVIFLTARDEVDDRVMGLDSGADDYLC
                     KPFAFAELLARVRALVRRRERPSSMILRQDDVSIDLVAQRAQRVGRTLDLTAKELALL
                     SFFLRHPAEVLTREQIYENVWDGDFDTTSNTFEVHLKELRRKLEALGPRLIHNRRGQG
                     YFLGKLPLGR"
     gene            complement(3210..6146)
                     /locus_tag="VHS97_11330"
     CDS             complement(3210..6146)
                     /locus_tag="VHS97_11330"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3448839.1"
                     /translation="MASASGPTELEYFPLSASGEQTVTVSRRPIAKPDLPVQPIPESF
                     GRYTVVRLLGRGGFGSVYLARDGELGRLVAIKVPRPGLLSSPEQVQAFLDEARIAAGL
                     VHPAIVAVYDVGRQGENDVFVVFEYVEGRNLASILKGDRLSPTQLVKLMVTVAEAVHH
                     AHKAGLVHRDIKPSNILIDQQGRPHIADFGLALSENPQDLRAREIAGTPIYMAPEQVL
                     GETHRLDRRTDIWALGVVFYLGLLGRPPFSGRDREELFDEILHRDPKPLRQISDSISH
                     ELERISLKCLSKRMADRYDTAGEFADDLKRWLVAEASTESSDMVQPPAKGPSRGVSSA
                     RIARKGLRAFDFEDADLFLTLVPGPRDRDGFPDSIRAWKRKIDERDPDRTFTVGLLYG
                     PPGSGKSSLVKAGLLPHLGSHVRSVYVEASSTGTESILLAGLRREMASLPADCRLDQA
                     AVIVRDHMATRRGSKVLLVIDQFEQWLQSHPDDTDGELIRALRHCDGRGLQALLLVRD
                     DFWMAITRFSRALEVALVEGVNAAPAEPFDPGHAKRVLAELGRDLGRFADEPASGAST
                     ERSRFLDKAVHELAGADGRVFPVRLTLFALMLRDRPWTTATLRELGGIEGIEVTFLEQ
                     AFSRPTAPPVYRIHQGAAQAVLTALLPNPSSDLRGRHRPRHLLQAAAGYADRPAEFAD
                     LIKILDNELRMVTPVDPTAARSEGEIGPHSTTTNSTFYQLTHDYLVPPLRRWLTRKER
                     ETRQGRAALELATITSLWRDRPEARRLPSLIEWIRIVGFTRHRTWTADQRRMMRAATY
                     HHLTRWALGLAIAGALAYANSLVLYRQRAEMTLEGALNADYQMLPDYFPAIAAHATIL
                     RPLLEKIENDPSTSRRHREVAGILLFRDRPTPDRAQFLIERLLSAEPDELGVIASALA
                     AHPLDANRSALEKVLHDETAQPAAQLRAACALAAIEPTAAARWGNVASALAEALIDEH
                     RRPLPK"
BASE COUNT         1308 a         1831 c         1917 g         1159 t
ORIGIN      
        1 agatccacgg ggtcataaaa agccctgtaa ataagtgacc gcgacgaatc agcttcaata
       61 cgaggtggta tcggttacgt gtcgtcgtca ctgcccgccg tgccggggcg ggacgagccc
      121 gatctccatt gcgacggttc cgagcataca tctcatgagt cgtcacagcc tcatacaaag
      181 aatccgtgtc cgccatcggt ttctccgttg gtaacgccag tcagtgtcgt aatgacgcga
      241 gggaccgatc gcggaaccgc tcctccgcgg ctccagcctc attgcggggt cgattgtgag
      301 caggacgtat cggcgactca agtgaatatt agttgaatga tccaagaatg tagattactt
      361 actctaaagg aatccttagt gtcgaccgaa gtcgatacaa ggcgtccagc tactcttggg
      421 atgcggaatc ctccctagcg cggctgcgcc cgaaaataaa gattttcttt ctatcggcga
      481 gccttgtacc cgacggccgg atggaggctg gccacttcgc gcagcgcgat ccaacgccaa
      541 gcaccagtag gtcatggcta tcaatagtta agtaatgttg cacgctcagt tgtgcgagag
      601 catggacgca tcggggaccg actaggggac gcagatggca tcggtcaatt cggggcgcgg
      661 tcgaggaatt gcggaagaat tttgatcaga gaactccgtc atcggcaaac gcacgtcgaa
      721 acggctgccg cgaccgattt cgctctgggc ggtgacagtc ccaccgaagg ctcttgcgat
      781 tcgcgccacc accgacaacc caagcccgac gccagggacc cgctggggag aagtcgagga
      841 tgatcgaaag aagggctcga agattctcgt caagtcctcc ggcgcgatac cgcagcccaa
      901 atcctcgacc acaagcaggg cggacccgtc ttcgcttgct gtcgcgagga cgaccggctg
      961 gccggagaaa ctgtacttgc aagcgttatc cagcaggttc tcgactaact gacctaacag
     1021 attcggctgc gccttgatcc acaatgggcc ttgttcatct gatcgaagaa caatatctct
     1081 cgagcgatgg ttcttgggtc ggctgtccag atatttcatg agccagcagt tcaggtcgag
     1141 acgctcgaac ttggtcaagg tcgagctatc agggcggctc aagaaaagaa gcgactcgac
     1201 gatttggccg agttcgacag ctcgcttatg ggctacatga atgacgtgac gatactgttc
     1261 gccggaacgg tcgtatcgct gagcaacctc aaggtgcccg atcatcacag caacaggtgt
     1321 gcgtaactga tgcgacgcct cgctgctgaa ccgccgctga cggtcgtacg cttcgtggag
     1381 ccgcgccagc aggtcgttaa aagctcgcct tagttcgtcc aactcatcgc gtgtcccaac
     1441 ttcggccaga gcccagccgg ggttagctgc gtcgagagct tgcgctgatt caaccagcct
     1501 tgtaaggggt tccatcgtct tccgcatgag cgagcgcgcg cagagggcgg ccgtgaacca
     1561 caccagaatg ctaatgcata ccagaaacga accgagcgac gttagcgtgg actcggtctc
     1621 ctcaagcgag ccgaacgcgg ccagcacgat ttcgtcgtga taactcttac catcgggcgt
     1681 gttttggggg cggtcgacgc ccgacggccg acccctggtc ggagcgacac gaacctgcgc
     1741 cgctcgccac cttcgacgct tcatgtcggt cacccgatcg ggcagcgtgc cagcgcccat
     1801 tgcagatacc catgagagcg gtagttcctc atcgggtaaa ttcgtcgggc ttgtcagcaa
     1861 acggccctga ccatccgtaa ccaaccaggt tgccgcatgc cggtcattcc atcgactttg
     1921 aggcaaacgc tttttccttg gctcccatct cacccagccc gagttcgggt ctacgcaggt
     1981 gtttaacacg ttcaaaatgg cggtaagccg atcgtcgatc tgacggttca gatagatccg
     2041 cgacgtgatg aagagggccg tcgagaaccc cacgagaatg agaccaagta taccgagcaa
     2101 caggattgag aagcggttag ataggctcat aatcaccggc cgagcggaag tttgcccagg
     2161 aaatacccct ggccgcggcg attgtggata agacgaggcc ccagtgcctc gagtttacga
     2221 cgaagctcct tgaggtgaac ctcaaacgtg ttggaagttg tgtcgaaatc accatcccac
     2281 acattttcat agatctgctc tcgcgtgagt acctcggcgg gatgtcggag aaaaaacgat
     2341 aacagggcca gttccttggc ggtgagatcg agcgtccgac cgacacgctg ggcgcgctgg
     2401 gcgaccaggt cgatgctgac atcgtcctgt ctgaggatca tgctcgacgg ccgttcgcgg
     2461 cgacggacca gggctcgaac gcgggcaagc aactcggcga atgcaaaggg cttgcaaaga
     2521 taatcatcag ctccgctgtc aagacccatg acccggtcgt cgacttcgtc cctggcggtg
     2581 aggaagatga caggggcctc cagcccggac cggcgaaacc ctcggagcag ggataggccg
     2641 tcttggtgag ggagcgacca gtcgagcaag accaggtccc acggacggct ttgtaacagc
     2701 tcgtacccct gcctcccgtc gacagcgtgc tcgacggaga acccttcttc cgccaaccct
     2761 gtcatcacaa attcagcaaa ctcgagttcg tcttcgatga ttaggatagt gcgagccatg
     2821 ttttcaccac gcatcaaggt ccgcgtgaag accgctcgtc ggttctcata accggaacgt
     2881 cttcccaacc aagaacccta gaaataccgc aatgagcgga ggcttctcgc gaagagttcg
     2941 tgaagaaagt gtgaggtttc aactgaactc agattagttg gacacaattc gcgacctcat
     3001 gttgccgaat ggggttactg cgatcttgaa aaagggcgga cgcgcgaatc atggtgaagt
     3061 tgcacgtgcg gccgaggcgc cgcgggtttc cccatggcgg cgagggcctc cgcggcgacc
     3121 gactgcgatg tccggtcgcg atcgccgtcg cgacagacat cgctcagcgg tgccaccaga
     3181 atctcgttga ccggctcgag caacgacatc tatttcggca aggggcggcg gtgctcgtcg
     3241 atcagcgctt cggccagtgc cgaggcgacg ttaccccatc tggccgcggc ggtcggctcg
     3301 attgccgcca gcgcgcaggc cgcgcggagc tgcgcggccg gctgggcggt ttcgtcatga
     3361 agtactttct cgagcgcgct tctgttcgca tcaagagggt gagcggcgag ggcggatgcg
     3421 atcacgccga gctcgtcagg ctcggcggac aagagccgct cgatcaagaa ttgagcccgg
     3481 tccggcgtcg gccggtctcg aaataacagg atcccggcga cttcacggtg gcgacgggaa
     3541 gtcgaagggt cgttctcgat cttctcgagc aacggccgga gaatcgtcgc atgagccgca
     3601 attgccggaa aataatccgg gagcatttgg tagtcggcat tcaaggctcc ctcgagtgtc
     3661 atctcggccc gttggcgata gaggacaaga gaattcgcat aagccaaggc gccggcaatg
     3721 gccagtccca gggcccaccg cgtcagatgg tggtaagtgg cggcgcgcat catccggcgc
     3781 tggtcggctg tccaggtacg gtgtcttgtg aatccgacga ttcttatcca ttcgatcaag
     3841 gacggcagcc ggcgcgcctc gggacggtcg cgccagagcg acgtgatggt tgcaagctcg
     3901 agggcggcac gtccctggcg ggtctcgcgc tctttacgtg taagccaacg acgcagcggg
     3961 ggaaccaggt agtcatgtgt aagctgatag aacgtgctgt tcgtggttgt cgagtggggc
     4021 cctatttcgc cttccgaccg ggcggccgtc gggtcgacgg gtgtcaccat gcgcaattcg
     4081 ttgtcgagga ttttgatcag gtcggcaaac tcggccggtc gatcggcgta gccggcagcg
     4141 gcttggagca ggtgcctggg ccgatggcga ccccgcaagt ccgaggaagg attgggaagc
     4201 agggccgtga ggacagcctg agcggcgcct tggtgaatgc gatacaccgg cggtgccgtt
     4261 ggcctggaga aagcctgttc gagaaaagtg acttcgatcc cttcgatccc gcccaattcg
     4321 cgcaaggtcg cggtggtcca cggccggtcc cgcagcatga gcgcaaagag ggtcaagcgg
     4381 acagggaaga ctcggccgtc cgctcccgcg agttcgtgga cggctttatc gagaaaccgg
     4441 cttcgctcgg tcgaggcgcc ggaggcgggc tcgtcggcaa atcgcccgag gtcgcggccc
     4501 aactcggcga gcacgcgttt cgcatgtccc gggtcgaacg gctcggcggg ggccgcgttc
     4561 actccctcga cgagcgcgac ctcaagcgcc ctcgaaaatc gagtgatggc catccaaaaa
     4621 tcatcgcgca ccagcaaaag ggcctgcaat ccccgaccgt cgcaatggcg cagcgcgcgg
     4681 atgagctctc catcggtatc gtcaggatgg ctctgcagcc actgctcgaa ctggtcgatg
     4741 acgagcagaa ccttcgatcc gcgtcgggtt gccatgtgat cgcggacgat caccgccgct
     4801 tggtcgagcc ggcaatccgc ggggagcgag gccatttcgc ggcgcaaccc agcgagcaag
     4861 atggattcgg tcccggtgga ggacgcttcg acatagacag accggacgtg cgatcccaag
     4921 tggggaagca aaccggcctt gacgagagac gacttgccgc tgccaggtgg gccatagagc
     4981 aagccaactg tgaatgtcct gtccggatcg cgctcgtcga tcttgcgctt ccaggcccga
     5041 atcgaatcag gaaaaccatc ccggtcacgc gggccgggga caagcgtgag aaaaaggtca
     5101 gcatcctcaa aatcaaaggc tcgcagccct ttgcgagcaa tccgcgcgga tgaaacgccg
     5161 cgggacggtc ccttggcagg cggctgaacc atatcgctcg attcggtcga tgcttccgcg
     5221 acaagccatc tcttcaggtc gtcggcgaac tcgcccgctg tgtcgtaacg atcggccatt
     5281 cgcttcgaaa gacacttcaa ggaaatgcgc tcgagctcgt gggaaatgct gtcgctaatc
     5341 tggcgaaggg gcttgggatc gcggtggagg atctcgtcga agagttcctc acggtcgcgt
     5401 ccggaaaacg gcggccggcc caaaagaccc agataaaaga ccacgcccaa tgcccagatg
     5461 tcggttcgac ggtcgagccg gtgcgtctca ccgagaacct gctcaggagc catgtagatt
     5521 ggcgtccccg cgatttcccg cgcccggagg tcttgcgggt tttcgctgag cgccaggccg
     5581 aagtcggcaa tatgcggccg accctgctgg tcgatgagta tgttggaggg tttgatatcg
     5641 cggtgaacca gccctgcttt gtgcgcgtgg tgcacggcct cggcgaccgt gaccatcagc
     5701 ttcacgagtt gcgtcggcga gagccgatct cccttgagaa tgctggcaag gttacgcccc
     5761 tcgacatact cgaagacgac gaagacgtcg ttctcgccct ggcgtcccac gtcgtaaacc
     5821 gccacgatcg ccggatggac cagcccggcg gcgatgcgag cctcatcgag aaaagcctgg
     5881 acttgttcag gtgaactcag aagtcctgga cgcgggacct tgatcgctac cagccggccg
     5941 agctctccgt cacgggccag atagacactg ccaaaaccac ccctgccgag cagcctcaca
     6001 accgtatacc gcccaaagct ctcgggaatg ggctggaccg gcaagtccgg cttggcgatc
     6061 ggacgacggc tgaccgtaac ggtttgctcg cccgaagcgg acagggggaa atattccaac
     6121 tcggtgggcc cagacgcgct tgccaagttc gacccaggct cgttcgagct atgagacgaa
     6181 gcaccggcat ctcgcgcgaa gaccagttcc gcggc
//
LOCUS       DATAQL010000650         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4189893, whole genome shotgun sequence.
ACCESSION   DATAQL010000650 DATAQL010000000
VERSION     DATAQL010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189893"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1084)
                     /locus_tag="VHS97_11335"
     CDS             complement(89..1084)
                     /locus_tag="VHS97_11335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3448840.1"
                     /translation="MPMISVVVPIHNEEESLIALHEEFDAVFAGGALGPVEFIFVDDG
                     SRDGSWGVLTALAHDDPRIHAIRFRRNFGKAAALTAAFGAARGDLVFTLDGDLQDDPA
                     EIPHFLAQLEQGFDVVSGWKKTRHDPWHKVYPSRVFNWMLSRVSGCNLHDHNCGFKLY
                     RAEVLREIDIYGELHRFIPVLAHARGFRVAEVEVRHRRRQHGLSKYGVSRLFKGFLDL
                     MTVRFLTRFSQRPLHVLGGIGLAFFGMGSLGMLYLALLWLDVRNRPIGGRPMLFYSIA
                     FLLVGVQLISLGILAELVTAYNIRAEDTYSVAETIPPRVSQAAVDEVGTQTRSRL"
     gene            complement(1075..2115)
                     /locus_tag="VHS97_11340"
     CDS             complement(1075..2115)
                     /locus_tag="VHS97_11340"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX3448841.1"
                     /translation="MARPRWQRLTIAMVKSAVALVVLWAVGRHVLRTWNDLRDHSLAF
                     KFDPGWLIGSGLLYLAGLSAYGRFYERVLRSSPTPVRLIPTLRAYLVSHLAKYVPGKA
                     MVVVVRAGMVVPFGARASTAAIATFYETLVMMASGGLIAAAGFAAARGTHRVGLTVPL
                     WGQVELPLYRLAAVCGLGSGLAFLILVVPRVFGRLAGLVSLPIPGVGPEAMPQITGRL
                     LGEGLLWSWGGWVFLGLSQLAVLRAFDPTTIGVSMAVSLAPAVIASVALATVAGFMVA
                     VLPGGLGVREGVLMSTLAPAVGSDRAVIASLLLRLVWVAAELVAAAILVPGFRTPPPT
                     ITTVSQSGPECP"
     gene            2381..2863
                     /locus_tag="VHS97_11345"
     CDS             2381..2863
                     /locus_tag="VHS97_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX3448842.1"
                     /translation="MAGQCDFRATRRSGPGGQNRNKVETAVILTHRPTGTIAQASERR
                     TQGENRRVAMHRLRLELALAIRRPIGHDADYRYSPSPLWRTRCRGGRINVNPEHDDFP
                     ALLAEALDVLSACDDDPKNAALLLGCSTTQLIKFLKSAPRALGSVNERRRVAGRHPLS
                     "
BASE COUNT          677 a          946 c          840 g          484 t
ORIGIN      
        1 cgcgaatcgg cgcgtatccg ggcgttgcgg tatcgggtca acttgattca tggtgcagtc
       61 tcaagaatcc gaaagcattg atctctagtc agagcctgct tcgagtctgt gtgccaacct
      121 catcgaccgc ggcctggctt actctgggtg gaatcgtttc ggcgacactg tatgtatcct
      181 cggcgcgaat gttgtatgcg gtgaccagct cggcgaggat gcccaggctg atgagttgga
      241 cgccgacaag caaaaatgcg atcgagtaga aaagcatcgg gcgacccccg attggccggt
      301 tgcgtacgtc gagccatagc aacgccagat agagcatgcc caggctgccc atgccaaaga
      361 acgcgaggcc gatgcccccg agcacgtgca atggccgctg gctgaaacgc gtcaggaaac
      421 ggacggtcat caggtcgaga aaccctttga acagccgtga tactccatat ttggacaaac
      481 cgtgctgtcg ccggcgatga cggacctcga cctccgcgac gcgaaagcct cgggcatggg
      541 ccaggaccgg gatgaatcga tgcagctcgc cgtaaatgtc gatctcgcgc aatacctccg
      601 ctcgataaag cttgaatcca caattgtgat cgtgcaggtt acagccgctc acgcggctga
      661 gcatccagtt gaagactcgg ctgggataga ccttgtgcca gggatcgtgc cgcgtcttct
      721 tccagccgct gaccacgtcg aatccctgct caagctgtgc cagaaagtgg ggaatctcgg
      781 ccgggtcatc ctggaggtcg ccgtcgagag tgaataccag gtcgccccga gcggccccaa
      841 acgcggccgt aagtgcagcc gccttgccaa aattgcgccg aaatcggatc gcgtggattc
      901 gaggatcatc atgggccagc gcggtcagga ccccccagga tccgtcgcga ctcccgtcgt
      961 ccacgaagat gaattcaacc ggcccgagtg cgccaccagc aaagaccgca tcgaactctt
     1021 cgtggagtgc gatcaggctt tcctcctcat tatgaatcgg aacgacgaca cttatcatgg
     1081 gcattcagga cctgactgcg ataccgttgt gatagttggc ggaggagtgc gaaaccccgg
     1141 aacaaggatt gcggcggcca ccagctcggc ggcgacccag accaaccgca acagcaacga
     1201 cgcgatgacg gctcgatcag aacctacggc aggcgccaag gtcgacatca gcacgccctc
     1261 gcgaacaccc agtccgcccg gaagtaccgc gaccatgaag ccggcaacag tcgcaagcgc
     1321 gacactggcg atcacggccg gtgccagact gacagccatc gaaacaccga tcgtggtggg
     1381 atcgaacgcg cgcaataccg cgagctgact caatcccagg aaaacccagc ctccccaaga
     1441 ccaaagcaaa ccttcgccca ggagccgccc cgtaatttgc ggcatcgcct cgggccccac
     1501 gccggggatg ggtaggctaa cgagccccgc tagtcggcca aacacacgag gaaccaccag
     1561 aatcaggaac gccaaaccag accctagacc acacaccgcc gcaagacggt agagcggcag
     1621 ctcgacttga ccccagagcg gtacggtcaa tcccacacga tgtgttccac gcgccgccgc
     1681 gaacccagcg gcggcgatca aaccgccaga ggccatcatc accaatgtct cgtaaaatgt
     1741 ggcgatcgcc gcggtcgacg cccgcgcacc aaatggaacc accatgccgg cgcgaacgac
     1801 gacaaccatt gccttgccgg gaacgtactt cgctaagtga ctcaccaggt aggctcgcaa
     1861 ggtggggatc aaccgaaccg gcgtcggact ggaccttagc actcgctcgt aaaaccgacc
     1921 gtaagccgag aggccagcca aatacagcag tcccgaaccg atgagccacc cgggatcgaa
     1981 cttgaacgca aggctgtggt cgcgcaagtc gttccaggtg cgtaacacat gacgcccaac
     2041 cgcccagagc acgacgagcg cgaccgctga cttgaccatc gcgatcgtca accgttgcca
     2101 gcgcggccga gccatgcggc gtacctcttc ctccgaattc acaatgcacc ggacccaagc
     2161 taccggcctg cccgaagacg aacaagtgag agcaactcat cggggcaatc cctgatcgat
     2221 cgcgggccac cgctggtaga ctgctttatc aaagaaactg ccgattcgta gaaggctcaa
     2281 aggcaggcct cttggagaac gatccattcg tacggacgac gctcaagaag cagacccggt
     2341 cgattcatcc gatcatccgg ccgcgctcga ccccgataag ttggccggcc aatgtgattt
     2401 tcgcgccacg cggcgctcgg gaccaggggg ccaaaaccga aacaaggtcg agacggcggt
     2461 aatattgacg caccgcccga ccggaacgat tgcccaggct tccgaacggc gaacccaggg
     2521 ggaaaaccgc cgggtagcaa tgcatcgact ccgacttgag ctggcactgg cgattcgccg
     2581 tccgatcggt cacgacgccg actatcgtta ttccccgagc ccgctttggc gtacccgttg
     2641 tcgcggcggt cgaatcaatg tgaaccctga gcacgatgac tttccggcac tgctggcaga
     2701 agctctcgac gttttgtccg cttgcgatga cgatcccaag aatgcggctc ttttgctcgg
     2761 ctgctcgacc acgcagctga tcaaattcct caagtcggcg ccccgtgcac tcggctcggt
     2821 caatgaacgc cgccgagtgg ccggccgaca ccctctctcg tgatgcgggc atccgtccgg
     2881 ccgctcggcg gcaatggcga cgtatagcct tactagttga gactttacag tcagcaaaag
     2941 cctctcg
//
LOCUS       DATAQL010000651         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4036665, whole genome shotgun sequence.
ACCESSION   DATAQL010000651 DATAQL010000000
VERSION     DATAQL010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4036665"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1058)
                     /locus_tag="VHS97_11350"
     CDS             complement(201..1058)
                     /locus_tag="VHS97_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3448843.1"
                     /translation="MLSWKVGAVTISCVVETVFRIPHDPQGWFLRDATPSALKASPWL
                     YPHFANEDGSLNVYVQALLVDTPGLKLMVDTCIGNDKPRGLTGGYPLATSFLQHLMDA
                     GWSRDSVDAVLCTHLHVDNVGWNTMLVDGKWVPTFPKARYLIGKREFEHWSNEADDEQ
                     QAIMGDSVQPIFDAGLVELVEMGYRISPEVRLRPTPGHTPGHVSVMIESEGQSAVITG
                     DIAHHPCQMAHPEWATSFDSDTQASTATRAKLFAEWADQPILVIGTHYAAPTAGYVKR
                     DGAAFRFAV"
     gene            complement(1104..>1877)
                     /locus_tag="VHS97_11355"
     CDS             complement(1104..>1877)
                     /locus_tag="VHS97_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3448844.1"
                     /translation="ESGFAVGSRVMFTGPYGVGENGTWQECLLVRPEHLALIPNAIGD
                     VVAASLPVAYLTAQVTLTLAGFKPGMTVLAPGIGGSVGNATYQLARAQGAGKVISTAG
                     SSAKAAKARELGFENVIDLSVGSLAEGVRHITAGKGVDIVIESIGATVTSEALSSLNL
                     GGVLITLGYSAGRKATIDVTDLIWKRARIAGFSLFAQSPMAIYAAWRDILPLILNGSV
                     KPVVEQVYPFAEAGHALRHLIEDRPFGKIVLADPSRAGR"
BASE COUNT          340 a          614 c          564 g          359 t
ORIGIN      
        1 gccgcggcat gagcggcggc ccgctccagc gacaccaaca gaacgtcgag tccgcgcaga
       61 aacacgggaa tggaagccct atacatcgaa agagtcatcg tttgtgtcct tacaggctcc
      121 gagccggcac caggaactaa acttctcgcc cttccggacg acctcgtctg ccggccgcct
      181 tcaccgaaag ggtttggcgg ctaaaccgca aatcggaacg ccgccccgtc acgcttcacg
      241 taaccggcgg tcggcgcggc atagtgggtg ccgatgacca gaatcggctg gtcggcccat
      301 tcggcgaaca acttcgcgcg cgtggccgtc gaggcttggg tgtcgctatc gaaactcgtc
      361 gcccactcgg ggtgtgccat ttggcaggga tggtgggcaa tgtcgccggt gatcaccgcg
      421 ctctgccctt ccgattcaat catcacgctg acatggcccg gcgtgtggcc gggggttggc
      481 ctcaggcgca cctcgggcga gatgcggtaa cccatctcca cgagttcgac caagccagcg
      541 tcgaagattg gctgcacact gtctcccatg atcgcctgct gctcgtcgtc ggcctcgttg
      601 ctccaatgct cgaactcgcg cttgccgatc aggtagcggg ccttggggaa ggtcgggacc
      661 cactttccgt ccaccagcat tgtgttccag cccacattgt ccacgtgcag gtgggtgcag
      721 agcacggcgt cgacactgtc gcggctccag cccgcatcca tcaggtgctg gaggaacgac
      781 gtcgccaatg ggtatcctcc cgtcagaccg cgcggcttgt cgttgccgat gcaggtgtcg
      841 accatcagct tcagccccgg cgtgtccacc agtagggctt ggacgtacac gttgagcgaa
      901 ccatcttcgt tggcgaagtg cggatacagc cagggactgg cctttagcgc cgagggcgtt
      961 gcatcccgta ggaaccagcc ctgcggatcg tgcgggatgc gaaataccgt ttcaacgacg
     1021 cagctgattg tcaccgcgcc caccttccag ctcaacatgg tgtacctctc ttgctcgctt
     1081 cacgccgtga atattccgac cgcttatcgt ccggcccggc tcggatcagc caagacgatt
     1141 ttgccaaacg gccgatcctc gatcagatgc cgaagggcgt ggcccgcctc ggcgaaggga
     1201 tagacctgct cgacgaccgg cttgacggag ccgttgagga tcagcgggag gatgtcccgc
     1261 catgcggcgt agatcgccat cggcgattgg gcgaacagcg agaagccggc gatgcgtgcg
     1321 cgtttccaga tcaggtccgt cacatcgatg gttgccttgc gtccggcgga ataacccaac
     1381 gtaatcagga caccgccgag attgaggctg ctcagtgctt cactggtcac ggtcgcgccg
     1441 atgctctcga tcacgatatc gacaccttta ccggccgtta tgtggcgaac gccctcggcg
     1501 aggcttccca cggataggtc gatcacgttt tcgaagccaa gctcgcgcgc ctttgcggcc
     1561 ttcgccgagc tgcctgcggt cgaaatcacc ttgccggcgc cctgcgctct cgccagttga
     1621 taggtcgcgt taccgactga ccccccgatc cccggagcca gcaccgtcat gcctggcttg
     1681 aatccggcca gtgtgagagt gacctgggct gtcagatagg ccaccggaag gctcgccgcg
     1741 acgacatcgc cgatcgcgtt ggggatgagc gccaggtgct caggtctcac caataggcac
     1801 tcttgccacg tgccattctc cccaacgccg tagggtcccg tgaacatcac ccgacttccc
     1861 acagcgaagc cggattc
//
LOCUS       DATAQL010000652         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1135975, whole genome shotgun sequence.
ACCESSION   DATAQL010000652 DATAQL010000000
VERSION     DATAQL010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135975"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHS97_11360"
     CDS             <1..264
                     /locus_tag="VHS97_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448845.1"
                     /translation="VRNAIGVLNGMVTTMQFAHDRFYLQEKELGRVINIDLTDVNVSA
                     TKFNLTDPDKDSLYTHGYQATRTFFLNKWSWKRHLAARGFPTP"
     gene            362..1318
                     /locus_tag="VHS97_11365"
     CDS             362..1318
                     /locus_tag="VHS97_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3448846.1"
                     /translation="MKSAGVVVETQEMRQEEPGRGDPIEPFDGHKIIPFGPDRSSDRL
                     GWGSVEAARYRVAPASECSTPAHTHHRLVLFARPTEELELLYEGVKRHVPPPAGSISL
                     VPAGTPALWRWSGRREGSLHIFLKPSLVEQVAAEAFDLDPARTTVPPLDGLDLPHLWA
                     AMGAVDAELTAGGPGGPLAAESLANVVAVHLIRHVVAPRRTARRPDGALPRSRLRAVV
                     EYIEGHLDASPTLEQMAAVAGVSTYHFAHQFKRATGLPPYQYVIMRRVERAEQLLRAG
                     TDLSLAEIAARAGFADHSVFCRHFKRLVGVTPGRFRMHTRIA"
     gene            1476..2918
                     /locus_tag="VHS97_11370"
     CDS             1476..2918
                     /locus_tag="VHS97_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448847.1"
                     /translation="MYEYGDFFRDASPDWESYITTGDEFGGFDSKLDFQGSRLWVTVR
                     LAWRFGHSFKRNKFGGSVLWSSQRQDQFKASFRNLILDYWDERYPIEIRHTPTGTMRT
                     VIPKFQLVDVPDGGGGQHMKIWVSALPAAQSSVGKRCKLHAYDADDDPSHSVLRRIHA
                     WGIKRRGVLKTMGQAIQDLAKGGVKFARDRVDLDAQGRDQLDRFAESLHVELPIFPAL
                     EIKVKSHRLPDENPALDGQRVAAVRAYLMGKGFPAKDLVEKPGGTVSGPLLSLVRGVA
                     AARPYVTLQYRKSITQKFIDGKYIYPIAAHEFGHMLGLPDEYHLSEKESSGQQGMHEN
                     YYHLCDKFHVPRPPRNTTSMSMMAGGNQLLRSHYVTLAQAVRQHLMRFYSQESLLNRL
                     RVNPTNGTQMTPKQLQDTWNTMDKDKPWDDYAITIGPMLRQPTKAEMVPLQRYIPLEA
                     TPDAVTVPDAFQHPSPELGPTFRADDFDLG"
BASE COUNT          674 a         1121 c         1068 g          566 t
ORIGIN      
        1 gtccggaatg ccatcggcgt cctcaacggg atggtcacga cgatgcagtt cgcacacgac
       61 cgcttctatc ttcaggagaa ggaactcggc cgggtgatca acatcgacct gaccgacgtc
      121 aatgtatcgg cgacgaagtt caacctgacc gaccccgaca aggattcctt gtacactcac
      181 ggttaccagg ccacccgcac gttcttcctt aacaagtgga gctggaagcg gcacctggcg
      241 gcccgcggat tcccgacgcc ctgaccgacg cgtcctagaa tcgaggggcc ggtagtcgcg
      301 gactcggcct aggggtgcca aggcgggcgg cgggtcacgc cgaaatacag acggagaatc
      361 gatgaaatca gccggagtcg tcgtggagac gcaagagatg cgacaggaag agccgggacg
      421 cggcgacccg atcgaaccct ttgacgggca caagattatc ccgttcggac cggacaggtc
      481 gagcgacagg ctggggtggg ggagcgtgga ggcggctcgc tatcgtgtgg cgcccgcctc
      541 cgaatgtagc acgcccgccc acacccacca ccggctcgtc cttttcgccc ggccgacgga
      601 ggaactggag ctgctgtatg aaggggtgaa gcggcacgta ccgccccccg ccggatcgat
      661 ctcgctggtg cccgccggca ccccggccct gtggcgctgg agcgggcgca gggagggctc
      721 gcttcatatt tttctgaagc cgagcctggt cgagcaggtc gccgccgagg cgttcgacct
      781 cgacccggcg cggacgacgg tcccgccgct cgacggcctg gacctcccgc acctctgggc
      841 ggcgatgggg gcggtcgacg ccgagctgac ggccggcggc cccgggggac ctcttgccgc
      901 cgagtcgctt gccaacgtcg tggccgtcca cctgatccga cacgtcgtgg caccccgccg
      961 aacggcgcgc aggccggacg gcgcgctgcc gcggagcagg ctccgtgccg tcgtcgagta
     1021 catcgagggg cacctcgacg ccagcccgac cctggagcag atggccgcgg tcgccggcgt
     1081 cagcacctac cacttcgcgc atcaattcaa gcgggccacc gggctgccgc cctaccagta
     1141 cgtcatcatg cgccgcgtcg agcgagcgga gcagctcctg cgagcaggaa cggacctttc
     1201 cctggccgag atcgcggcgc gcgccggctt cgcggaccac agcgtcttct gccgtcactt
     1261 caagcggctg gtcggcgtca cgccggggcg gttccggatg cacacaagaa tcgcctaaac
     1321 ggccgcaatt cccgccaaga atccgggcgg cgaccccctt accattcctc acgagcaggg
     1381 tgcgtcggcg tggtggcgcc gcgggcgagg gccgaagcac ctgcgtgacg atttgaccta
     1441 tcccgaatgg acgatcgagc gaaggagagt cgagcatgta cgaatacggc gattttttcc
     1501 gcgacgcctc cccggattgg gaaagctaca tcaccaccgg cgacgagttt ggcggcttcg
     1561 actcgaagct cgacttccaa ggcagccgcc tctgggtcac cgtgaggctg gcctggaggt
     1621 tcgggcactc cttcaagcgg aacaagtttg gcggcagcgt cctctggtcc tcgcagcggc
     1681 aggaccaatt caaggctagc ttccgcaacc tcatcctgga ttactgggac gagcgctacc
     1741 cgatcgagat ccggcacacg ccgacgggca cgatgcgcac cgtcatcccg aaattccaac
     1801 tcgtggacgt gccggacggc ggcgggggtc aacacatgaa gatctgggtg tcggccctgc
     1861 cggccgcgca aagctccgtt ggcaagcgat gcaagctgca tgcctacgac gccgacgacg
     1921 accccagcca ttccgtcttg cgccgcattc atgcctgggg gatcaagcgg cgcggcgtct
     1981 tgaaaaccat gggtcaagcc atacaggacc tggccaaggg cggggtcaag ttcgccaggg
     2041 accgcgtcga cctcgacgcc caggggcgtg atcaactcga ccgcttcgca gagtctctgc
     2101 atgtcgaact accgatcttc cccgcgctcg aaatcaaggt caaatcccac cgcctcccgg
     2161 acgagaaccc cgccctcgac gggcagcgcg tcgccgcggt gagggcctac ttgatgggca
     2221 aagggttccc ggcgaaggat ctcgtcgaga agccgggcgg cacggtttcc ggtccgttgc
     2281 tgtcgctagt ccggggcgtc gccgcggcgc gcccctacgt cacgctgcag taccggaagt
     2341 cgattacgca gaaattcatc gacggtaaat acatctaccc gatcgccgcc cacgagttcg
     2401 gacacatgct cggcctgccg gacgagtacc atctttcgga gaaggagagc agcggacagc
     2461 aagggatgca cgagaactac taccacctct gcgacaaatt ccacgtccct cgcccgccga
     2521 gaaacacgac ttcgatgagc atgatggcgg gcggcaacca gctgctccgt tcccattatg
     2581 tcacgctggc ccaggccgtg cgtcaacacc tcatgcgctt ctacagccaa gagtcgctcc
     2641 tgaacagact ccgcgttaac cccactaatg gcacgcagat gacgcctaaa cagctccagg
     2701 acacctggaa cactatggac aaagacaaac cctgggatga ctacgcgatc accatcggcc
     2761 cgatgctgcg ccagcccact aaggcggaga tggtgcccct ccaacgctac atccccctgg
     2821 aggccacccc cgacgccgtc acggtcccgg acgcgtttca gcatccgtcg cccgaattgg
     2881 ggccgacgtt cagggcggac gacttcgatc tcggctgata gggaacagga agaggggtgg
     2941 taaggcgaaa aggtctattc cccccatact tacctgaact ggagttgcca ggtcccttgc
     3001 aatggccttg accgcggcgt tgtgttgtta cccggtgagg gaccctgagt cgtttcaggg
     3061 tacaccgcac tttcttcgct acacactcgg agggcaatcc caatgggcgt cgaacagaag
     3121 cttatcgtcg ggagatttct cgaatttaaa ttgccagaca tccgaccgcc acagaatcct
     3181 caggggaaca tcgtatacca gagtgtcatg aatatgacgc ttttggaatc gaccggtact
     3241 tttcaaaagc aaagcaagat cgtcgagggc gtgagccaaa aggtgcatcg gaatttccag
     3301 tggcttgcgt ggatgcccgg cgcgatcagc gctgtacaac ttcagtcttg accgcgaggg
     3361 gacctggcac gtcggaccga ggactggccc ggcggacctc ttcgatcccc ggcggcggag
     3421 gcggtttgg
//
LOCUS       DATAQL010000653         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2358567, whole genome shotgun sequence.
ACCESSION   DATAQL010000653 DATAQL010000000
VERSION     DATAQL010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358567"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VHS97_11375"
     CDS             <1..433
                     /locus_tag="VHS97_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448848.1"
                     /translation="GESPWVSVSLSGRAKGLLRTGNNASSGLRLAAVAIAPVVGGQPP
                     SVSMSLPPGGIGALGHLIYCQDAAAPPAVLPAGCYVLVMDFAIEAVVSSHFHDFAGAA
                     FSKARSGIHLMANNPFQGLDSGDLGFTAVLKAESIAVAPEP"
     gene            complement(771..>2264)
                     /locus_tag="VHS97_11380"
     CDS             complement(771..>2264)
                     /locus_tag="VHS97_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3448849.1"
                     /translation="LGMPPGTAGGGSAFGGGDPTTVGTQVVGNRGRGAMPLLDGMLDS
                     GDLSSSMAGSGMTPGTAGGGSASGGGDPTTTGRAQVVGNRQRGRIPLMGGSYDFANDG
                     FRWGTEDDEITFGVRAVQQLDARIYANSNMADTASGFYNPRTRFYFEGNLTRPISYEF
                     SFQETYGTPNLLDSYINYRFSDALQLRLGRYKTPFTYEWYRVHIWHMPLPERSLFVTN
                     YESNRRFGFMAWGSAFQNRLEYAVGPFNSQRNSFVTYSSHQDILAFVNFKPFDPWTGF
                     LLQNLQVGGSVDAGIVNNPLSPAVLHTSSPVDSATLAPGQPSVPFLAFNNGVFERGTR
                     ALWELHMAYYYQGLSLLGAWGSGFQDYAFGTSGSAPVRVPISGYQVTAAYILTGETIR
                     DRTLVDPLRPFDLRPGRFGLGAWEVAARFSELELGRQVFTGGLANPNLWTNEAKMVDV
                     GLNWYINKAIKVYFDWEHAMFGSPVFYNTGRFQKSNDLYWMRAQVVF"
BASE COUNT          402 a          717 c          748 g          397 t
ORIGIN      
        1 gggggagagc ccctgggtta gcgtgtcgct ctccgggcga gcgaaaggct tactacgcac
       61 cggaaacaat gcctcctccg gtttgcggct ggccgccgtc gcgatcgccc ccgtggtggg
      121 cggccagccg ccgtccgtgt ccatgtccct gcctcccggc gggatcggcg ccctcggcca
      181 ccttatctac tgtcaagacg ccgcggcgcc gccagcggta ctgccggccg gatgttacgt
      241 cttggtgatg gacttcgcca tcgaggccgt ggtttccagc cacttccacg acttcgccgg
      301 ggccgcattc tcgaaggccc gcagcggtat ccatctcatg gccaacaacc cgttccaagg
      361 tcttgattcg ggggacctcg gcttcacggc tgtcttgaag gcggagtcaa tcgccgtcgc
      421 gccggaaccc tgatccaaag ttccggctga tggtgtcggt ttcaccggcc agtgactggg
      481 ccatcgctcg gtcgcaaccg gcgcaaatcc cgacggtgcc gtcggtgagc ccagggtcca
      541 gcccgcaagg caagcgagag caagctcgga ttcttcacgc gcctacgccc tcctcgtaag
      601 cccgtccgag cgactcccag cgcgaccgcg ctgacgggcg cacgggatga ccagttgatg
      661 ccgggcgcgc ctggcgatcc gaggtcggac ccgtccggat cgtgatgccg atcgtcgtct
      721 cgcgcggcca tcaacagccc cggggctgga gtgggcccag ccgttgggga tcagaacacg
      781 acttgggccc gcatccagta caggtcgttt gacttctgga atcgacccgt attgtagaag
      841 acggggctgc cgaacatcgc gtgctcccag tcaaagtaca ccttgatggc tttgttgata
      901 taccagttga gtccgacatc gaccatcttg gcttcgttgg tccagaggtt cgggttggcc
      961 aggccaccgg tgaacacttg ccggccgagc tcaagctcgc tgaagcgggc cgcgacttcc
     1021 caggctccca gaccaaaacg gcccgggcgg aggtcgaagg ggcgtagagg atcgaccagt
     1081 gtccggtcgc gaatcgtctc gccggtcaag atgtacgcgg ccgtgacctg atagccgctt
     1141 atcgggaccc ggacgggcgc agaccccgaa gtgccgaagg cataatcctg gaacccgctg
     1201 ccccaggcac cgagcaagga gagcccctga tagtaatagg ccatgtgcaa ctcccacagg
     1261 gcgcgggttc cgcgctcgaa cacgccgttg ttgaaggcca agaacgggac cgacggctgc
     1321 cccggggcga gcgtggctga gtctaccggc gacgaggtgt gcaagacggc cggcgagagg
     1381 ggattgttca cgatccccgc gtcgacggag ccgcccacct gcaggttttg gagcaagaaa
     1441 cccgtccacg ggtcgaatgg cttgaagttg acgaaggcca ggatgtcctg gtggctcgag
     1501 tacgttacaa acgagtttcg ctggctattg aacggcccga ccgcgtactc cagtctgttc
     1561 tggaacgcgc tcccccaggc catgaatccg aagcggcggt tgctttcgta gttggtgacg
     1621 aaaagcgagc gttccgggag gggcatgtgc cagatgtgga cccgatacca ctcataggtg
     1681 aagggggtct tgtaccggcc gagccgaagc tggagggcgt cgctgaaccg gtagttgatg
     1741 tagctatcga gcaggttggg ggtgccgtat gtctcctgga acgaaaactc gtaggagatg
     1801 ggccgggtca ggttaccttc gaagtaaaat cgggtccgcg ggttgtagaa gccgctggcg
     1861 gtatctgcca tgttcgagtt ggcatagatc cgcgcgtcga gctgctgcac cgcccggacg
     1921 ccgaaggtga tctcgtcgtc ttcggtaccc cagcggaagc cgtcgttggc gaagtcgtac
     1981 gagccgccca tcaaagggat ccggccgcgc tggcgattgc ccacgacctg ggcacggccg
     2041 gtggtcgtgg ggtcgccgcc gccggatgca gatccaccgc cggcggttcc cggagtcata
     2101 ccggaacccg ccatggatga actcaaatca ccggagtcta acatgccgtc caagagcggc
     2161 atcgcaccgc gcccgcgatt gcccacgacc tgagtgccga cggtcgtggg gtcgccgccg
     2221 ccgaacgccg atccgccgcc ggccgtcccc ggcggcatgc cgag
//
LOCUS       DATAQL010000654         7898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2509086, whole genome shotgun sequence.
ACCESSION   DATAQL010000654 DATAQL010000000
VERSION     DATAQL010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2509086"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="atpH"
                     /locus_tag="VHS97_11385"
     CDS             complement(<1..582)
                     /gene="atpH"
                     /locus_tag="VHS97_11385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX3448850.1"
                     /translation="MTDSSAAGVVEPRVSGETAELARPYALALVNAAEKEGGIEPVLD
                     ELDQIDNDVLKVFPRFASLLGSARVSSAHKDRILTDVFEKRASSVVLRFLRVLNRHER
                     LGLLRVVAREARTIWDQRNKRIPVQVRAAISLDEKQLQTLRDRLAALTGATPVLSVST
                     DPGLIGGLVVQVGDHRFDASVKSRLEQLRQRLIE"
     gene            complement(579..1280)
                     /gene="atpF"
                     /locus_tag="VHS97_11390"
     CDS             complement(579..1280)
                     /gene="atpF"
                     /locus_tag="VHS97_11390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX3448851.1"
                     /translation="MMRQTKRAISLGLAAFWLAWLTTGGLLMAREMQEHKAGHTGAEA
                     KMAKAEHVEAEPEEKINPLEVKPALAVWTLVVFLLLLFVLGRFAWKPLLAALHNREQH
                     LEHVLLETERARNESEANLAEHRKLMARAADEVRGILEKARKEAEATGEQLIKQAQGE
                     AELAKQRAQRDIASARDQALAEIWEKTADMAVSVAGKVLSKELSDTEHRRLLEAAINE
                     LPAQAASNGHGGKGS"
     gene            complement(1441..1770)
                     /gene="atpE"
                     /locus_tag="VHS97_11395"
     CDS             complement(1441..1770)
                     /gene="atpE"
                     /locus_tag="VHS97_11395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX3448852.1"
                     /translation="MKLIKLVVLACGLFLLAGGSAFAQGQPASGAGPAAGPAPFSDLK
                     AVGAGMVIIGAAFGIGTLAKGAVESMARQPEIAGNIQTAMIIAAALIEGVTFFALVII
                     IVQVLFG"
     gene            complement(1977..2804)
                     /gene="atpB"
                     /locus_tag="VHS97_11400"
     CDS             complement(1977..2804)
                     /gene="atpB"
                     /locus_tag="VHS97_11400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX3448853.1"
                     /translation="MAEHSENPLAHVIDHPTITVPWWNPPSFEQTFVLRKIAGFQITH
                     FMVMELIAAVLMVLILVPVVRHIARTPYSRGWFMNMFEAMLLFIRDDVARPAIGGHGA
                     DRYLPYLWTVFFFVLFNNLLGMFPGGASATGNVNVTAVLALMTLAVVLGAGMREMGVV
                     GYWLGIVPHMDVPAWLKPPLWGLMFVIEVAGLLIRHVVLAVRLFANMFAGHVVLAVIL
                     GFILMTKFLAPSFFLVTPASLIGVVLLSLLELFVAFLQAYIFTFLSALFIGSAVHPH"
     gene            complement(2899..3195)
                     /locus_tag="VHS97_11405"
     CDS             complement(2899..3195)
                     /locus_tag="VHS97_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX3448854.1"
                     /translation="MVDQRESQSLLSVGIAWATRITTIALELSVPVAVGFGLDRWLST
                     APVATILGALLGFVLFMLHTVRIASEFSRGSNPKAAGLDDERRRAGRGGPSGSG"
     gene            4006..5085
                     /locus_tag="VHS97_11410"
     CDS             4006..5085
                     /locus_tag="VHS97_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HEX3448855.1"
                     /translation="MTADALNDCSKRHLAQLAKEKGINGWHAMRKDQLIKALTSARSS
                     ASNTRKKKIIPVARAARRKKPASAAAGRSNATVAAATPTQTLDHASQKDRIIVLTRDS
                     YWLHAYWELSRTTLARARAALGQDWHAARPILRVMDVSSEDTTSAAERHIRDIPIHGG
                     VNNWYVDVLEPPRSYRIDIGYLSRRGRFYVLARSNVVTTPKAGVTDTLDENWSNVQKQ
                     FERIQNPSSNGNGNGTTHHNTQIDLRDLFEERLRRPMNSASLQNLATTAMPHLGRDFH
                     FQIDAELIVYGTTEPNARVTLQGEPVQLRNDGTFTVRFSLPDSRQIIPAVASSADGVE
                     ERTIVLAVERNTKELEPMIHDNNEI"
     gene            5372..6133
                     /locus_tag="VHS97_11415"
     CDS             5372..6133
                     /locus_tag="VHS97_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX3448856.1"
                     /translation="MSQDIHGILDGWEFEPEGLQVRIIVGDDGNERIQMRIDLGLIQM
                     EMDGRPDGQRPDGFPALLDSYEAKARDALTSGAPFSLTADDCALLMREGLQYYHRYLS
                     AFHLERYDLVERDTERNLRLFSFVARHAARQRDKLDFEQYRPYVQMMHTRARATQALK
                     KGEHRGALKRIDEGIKAIRKFLAEYQQQEKEEDCSELRFLLRWRREVERERPLGALER
                     LEHQLELSVELEDYEQAARIRDQIRLLQESTRERN"
     gene            6509..7126
                     /locus_tag="VHS97_11420"
     CDS             6509..7126
                     /locus_tag="VHS97_11420"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX3448857.1"
                     /translation="MSAHDHDVPGNSRSKAELRQAIKAAIARLDPAVRRAQESALMGR
                     FGQLPGLLEAASILLFVPAFPEEPPMVELLFSTYEMKKDVYLPRVDKAARRLRLCRVS
                     DPASDLCPGALGISEPRPGLPEVPPETIDWALVPGLAFDELGFRLGRGGGYYDRLLPM
                     LRPQAICWALCLSCQLVGRLPVEAHDAPLNGVSTPERDVFGMRGA"
     gene            complement(7123..>7898)
                     /locus_tag="VHS97_11425"
     CDS             complement(7123..>7898)
                     /locus_tag="VHS97_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEX3448858.1"
                     /translation="PTGESYTREYPLSIRADEDHDHPHQRSCWFTFGNVNGIDFWSEG
                     KRFGTVKENERTLVVAGPVIGRLKTLNEWRAADDKKVCTDERLVTFYRTKSARLIDFK
                     VRIIASAGPVEFRDTKEGMFGIRVASSMDVDKKTGGKITNAEGLTDDKAWGKPSPWVD
                     YVGPVKDRTVGIAIINHPTSFRYPTTWHVRTYGLFAANPFGWHDFGRPDKGNYMIEDG
                     QAIEFSYRVVLHDGDTNSAGVPAIANAYAKPPTVEVVKN"
BASE COUNT         1697 a         2441 c         2238 g         1522 t
ORIGIN      
        1 ttcgatcagt cgttggcgaa gctgttcgag tcggctcttc accgacgcgt caaaacggtg
       61 gtcaccgacc tggacgacca gtcccccgat caggcccggg tcggtcgaga cggagaggac
      121 cggcgtggcg cccgtcaggg ctgccagtcg gtcacgcagt gtttgcaatt gcttttcatc
      181 aagcgaaatg gccgcgcgaa cctgaaccgg gatccgcttg ttgcgctgat cccagatcgt
      241 ccgggcctcg cgagcgacga cgcgaagcaa gcccagccgc tcgtggcgat tcaagacccg
      301 caaaaaccgc aataccacgc tcgacgccct cttctcgaag acatcggtga ggatccgatc
      361 tttgtgcgcg ctcgagacgc gcgccgaccc caatagtgag gcgaagcgcg gaaagacttt
      421 caagacgtcg ttgtcgatct ggtcgagctc gtccaggacg ggctcaatgc ctccctcttt
      481 ctcagccgcg ttgaccagag ccagggcata cgggcgggcc agttcggcgg tctcgcccga
      541 aacccgcggc tcgaccacac cggcggcgct cgagtcggtc atgagccctt ccctccgtgc
      601 ccattggaag ccgcctgggc cggcaactca ttgattgcgg cctcgagcaa ccggcgatgc
      661 tcggtgtcgg agagctcctt ggagaggacc ttaccggcca cggaaaccgc catgtcggcc
      721 gtcttctccc agatctcggc cagggcctgg tcacgagccg acgcgatgtc gcgctgagcc
      781 cgctgcttgg ccagctcggc ctcgccctgg gcctgcttga tgagctgctc gccggtcgct
      841 tccgcttcct ttcgggcttt ttccaggatg cctcggacct cgtcggcggc ccgggccatc
      901 agcttacggt gctcggcgag attggcctcg ctctcgttgc gcgcccgctc ggtctccagc
      961 aggacatgct caaggtgctg ctctcggttg tgaagggccg ccaggagagg cttccaggca
     1021 aaccgaccca gaacaaagag cagcaagaga aaaacgacca gagtccaaac cgccagcgca
     1081 ggcttgacct caagcgggtt gattttctct tccggttccg cttcgacgtg ctcggccttg
     1141 gccatcttgg cctcggcgcc ggtgtggccg gccttgtgtt cttgcatctc tcgcgccatg
     1201 agcaggccgc ccgtcgtgag ccaagccagc caaaacgccg cgagtcccag tgaaatagcc
     1261 ctctttgttt gtcgcatcat ggaaaatttc ccgcgccgga cagccaaccg agacgatgct
     1321 ggagaaatac tcttaccgaa gacgattgag agatgtgccc cggggccagg tctatccggg
     1381 atcaggtcgc gcccatggcc ctcggacgcc ggctgacgag gtcttggccg gccgtcgcgg
     1441 ttagccgaac aagacctgga cgatgatgat aacgagcgca aagaacgtca caccttcaat
     1501 caaggccgcg gcaatgatca tagccgtctg aatgtttccg gcgatttcgg gctggcgggc
     1561 catgctctcg acggcgccct tggccagagt accgatgccg aaggccgcac cgatgatcac
     1621 catccccgcg ccgaccgcct tcaaatcaga gaacggagcg ggtccggctg ccggccccgc
     1681 tccggacgca ggctgcccct gcgcgaacgc cgatccgccg gccaagagga aaaggccgca
     1741 ggccaaaaca acaagcttga tcaacttcat ctgactctcg gctccttacg cttctaagag
     1801 acacacttca gtcatcgatg agcgggtcat tgaaattgcc cgccggccgc gacgaaacca
     1861 cactggcttc gacttcagac attcactaat taaaatcagt tccggccgct ccgtcgctcg
     1921 accgggttgc gagagccact cgctaagaga acggcaagca ctcgctcatt tgctttctaa
     1981 tggggatgga ctgccgaccc aataaaaagt gctgacaaaa atgtaaaaat gtacgcttgc
     2041 aagaacgcca cgaacaactc gagaagactc aagagaacca cgccgatcaa actggccggc
     2101 gtcaccagga aaaacgacgg ggccagaaac ttcgtcatca gaatgaagcc gagaatgacc
     2161 gcgaggacca cgtgaccggc aaacatgttg gcgaataatc gaacagccag gaccacatgc
     2221 cggatcaaga gccccgcgac ctcgatgacg aacatcaatc cccatagcgg aggcttgagc
     2281 cacgcgggga cgtccatatg cggcacgatc cccagccagt agccgaccac gcccatttcg
     2341 cgcatcccgg ctcccaacac gacggccagc gtcatcaggg cgagtaccgc cgtgacgttg
     2401 acgttgccgg tcgccgatgc gcctccaggg aacatcccga gcagattgtt aaacaagacg
     2461 aagaagaaaa ccgtccacag atacggcaag taacgatcgg cgccatggcc gccgatcgcc
     2521 ggccgggcga cgtcgtcgcg aatgaagagc agcatcgcct cgaacatgtt cataaaccag
     2581 ccacggctgt agggcgtacg ggcgatgtgg cgaaccaccg gtaccagaat caagaccatg
     2641 agcacagccg cgatcaactc catgaccata aaatgcgtga tctggaagcc agcgatcttg
     2701 cgcagaacga acgtctgctc aaacgaaggt gggttccacc acggcacggt gatggttggg
     2761 tgatcgataa cgtgagccag gggattctca gaatgctcag ccatggtgtt tgacgaacga
     2821 acaaggtgaa agttcgattg agagactctg aatcagtccg acttcactcg cggacagccg
     2881 gatttcccct cgtggtaatc agccagaacc gctgggcccg ccgcgaccgg ctcgtctacg
     2941 ttcatcgtca agaccagctg ccttagggtt cgacccgcgg gaaaactcac tggcgatccg
     3001 aaccgtgtgc agcatgaaca gtacgaaacc gagcaatgca cccaaaatcg tggccacggg
     3061 agccgtcgac agccagcgat ccagaccgaa accgacggcc acgggcacgg acaattccag
     3121 ggcgattgtc gtgatccgtg ttgcccaagc aatgcccacc gagagcaaag actgcgactc
     3181 tcgctggtcg accacgcacg actcccaact cgccccgttc cgcgcaggaa gccagatcgc
     3241 cgccagcaac cacacctgtc agaaagtacg gcgggttggc cgggctcgac ccagttgcct
     3301 cgcgaatgag gacgagcatt gactgttaga gcatcagttt tactgtgcgt atttcccctg
     3361 tcaactgctc accgccccag ttcgagaaat ttttcacaaa gtctcttatg cgtatacttt
     3421 aattgataaa aaccgatttc tgcgcgatgc tccgccggac gcacggagga taagcatcct
     3481 cgagtttcgc cgcgaagctc ggctaaactt gcggacaagc cgcgatctct cgattcccgg
     3541 cactgactgt ccgatttcgt acaccggtct ggaaaacgac gattgacaga gtttctctgc
     3601 ttatggtatc tgataagcag cgatttcaag caacgggcta gacttccgaa acgacgctcc
     3661 tcgatccttc cggttcgatc cagtccgggg acccgtcatg cttttcgatt tgcgcgcaga
     3721 gtctggataa gatgagactc gaaggcggca tcgaccggct tagccgggca cgatggccgc
     3781 tttttgtgtt tcggaagtat gacgacttga ccctgggcgg tggaacactc tcgacgctgc
     3841 gatcgctcga ctcggtggcc cttcgaggca cccggcttcg gcctgcctaa gtccggaaga
     3901 catggagttt tctggatcgg tccactcacg gatgaatgtc attaaattac attcatctct
     3961 cgactgggcc gacaaggcaa gcggggggtg ttggagtggt accgtatgac tgccgatgca
     4021 ctcaatgatt gtagtaagag acaccttgcc caactggcca aggaaaaagg aatcaacggc
     4081 tggcacgcca tgaggaagga ccagttgatc aaggcgctaa caagcgcgcg ttcgtccgcc
     4141 agtaatacca ggaaaaagaa gataattccg gttgcccgtg ccgcccggcg caaaaaacca
     4201 gcgtcggctg ccgccggcag gtcgaatgcg accgttgcgg ccgcaacacc gactcaaaca
     4261 cttgatcacg ctagtcaaaa agaccgaatt atcgtcctga cgcgcgactc ttactggctg
     4321 catgcgtact gggaattgag ccgtaccacc ctggcacggg cccgagcggc gcttggccaa
     4381 gactggcacg ccgcccgtcc catcctccgt gtcatggatg tctccagcga agacacaacc
     4441 tctgcagccg aaagacacat tcgcgacatc cccatccacg ggggcgtcaa taactggtac
     4501 gtcgatgtcc tcgagccgcc gcgatcttat cggatcgata ttggctacct gtcgcgtcga
     4561 ggacgatttt atgtactcgc ccgttccaac gtcgtcacca caccgaaggc aggtgtgacc
     4621 gacactctcg acgaaaactg gtcgaacgtt cagaagcagt ttgagcgcat tcagaatcca
     4681 tcgtccaacg gcaatggcaa tggtaccacc caccacaaca cccagatcga cctgcgggac
     4741 ctctttgaag agcggcttcg ccggccgatg aatagtgcgt ccctgcaaaa tctggcgacc
     4801 acagccatgc cgcacctcgg gcgcgatttc cattttcaga tcgacgccga gctgatcgtt
     4861 tacgggacca cagagccaaa tgcgcgcgtg accctccaag gcgagcccgt ccagctacgc
     4921 aatgatggaa cgtttaccgt tcgcttcagc ttacccgact cgcgacaaat tatcccggcg
     4981 gtggcctcaa gcgctgacgg agtcgaggag cgcacaatcg tgctcgccgt ggaacgcaat
     5041 accaaggaac tcgagccaat gattcacgac aacaacgaaa tctagtgtta cgtggcgcgg
     5101 ttgacccgcg agacaggacg atcccgttgg ccgtcgtggt gctacgtaac acctcgtcag
     5161 ttagtcttaa tacacagtcg ccgcgcgttg gctttactca aagaggccga tgcgcggcgt
     5221 cctttattcc atcgaattgg ctcgggcgtt cagtccggta cccccaccat tccctccttc
     5281 gtataatttc ttgtgttggc cgatcaatcc tcgagtggcc acctggccgc ccgtccctgc
     5341 acacgcactt ctggaacggg agaaagcacg cttgagccaa gacattcatg gcattctgga
     5401 cggttgggaa ttcgagcccg aggggctaca ggtccgcatc atcgttggtg acgacggtaa
     5461 cgaaaggatt cagatgcgga tcgacctggg acttattcag atggaaatgg acgggcggcc
     5521 ggacggccag cggcccgatg gcttccccgc tctcctggat tcttacgaag ccaaggcgcg
     5581 cgacgcactt acatcgggcg cacccttttc attgacggca gacgattgtg ccctcttgat
     5641 gcgcgaagga ttgcagtact atcaccgtta tctttcggcg ttccatctcg aacgctacga
     5701 tctggtggaa cgagacaccg agcgcaacct ccggctcttt tcctttgtgg cacgacatgc
     5761 cgcgcggcaa cgcgataagc tcgatttcga gcaataccgc ccttacgtcc aaatgatgca
     5821 cacgcgagcc cgcgcaacgc aggcgctcaa gaaaggcgaa cacagagggg cgctcaagcg
     5881 gatcgacgaa ggaatcaagg cgatccgcaa attcctcgcc gagtatcaac aacaagagaa
     5941 ggaagaagac tgctccgagc ttcgcttcct cttgcgctgg cgccgtgaag tcgagcgcga
     6001 gcgtccgctg ggagcgctcg aacggctaga gcaccaactc gagctttccg tcgagctcga
     6061 agactacgag caagccgcgc ggatccgcga ccagatccgg ctgttgcagg aatcgacacg
     6121 cgaacgcaac tagcggcgcg cgccttgcta ccagcgcttc gccaactgcg atcgggtacg
     6181 cggaatgtcg cgcggttgcg attaaatgcc tcacaccttg aatgccaccc ggacgctcgt
     6241 tacattacag aagtctggtt tatccggacg cagcctctgc catgttcgat aagaggtggt
     6301 ccttttggcg aaccaacgta aaacccgtcg ggatccaatg tcggctccta ggaacttgct
     6361 tcctcgcgtg cctcaggatc ccacctaaac tgcgggttct caaaagcacc tcaccggcat
     6421 tgcggtggag gtttgtcttt gcccgtgcct tccctccttc gtgaggtgag acacgggcat
     6481 tcttgttttc acgacagagt ggccaccatt gagcgctcac gatcacgacg tgccggggaa
     6541 ttcgcgctca aaggccgaac tgaggcaggc catcaaggcc gcaatcgcgc gcctcgaccc
     6601 ggccgttcgg cgcgcacaag agagcgcgct catgggccgg ttcggccaat tacccggcct
     6661 gttagaagcc gccagcatct tgctattcgt gccggcgttt ccggaagagc cgccgatggt
     6721 cgagctgctt ttctctacct atgaaatgaa gaaagatgtc tacttgccgc gcgtcgataa
     6781 ggccgcacgg agattgaggc tttgtcgagt ctctgacccg gccagtgatc tctgccccgg
     6841 ggccctgggt atctctgaac cgcgaccggg cttgcccgag gtgcctccgg agacgatcga
     6901 ctgggcactc gttcccggcc tcgctttcga cgagctaggt ttccgacttg gccggggagg
     6961 tggatattac gaccggcttt tgcccatgct gcgccctcaa gcgatttgct gggcactctg
     7021 tctaagctgc cagttggttg gccggttgcc agtcgaggcc cacgacgcgc cgctgaacgg
     7081 agtttcgacg cccgagcgcg acgtcttcgg aatgcggggc gcctagttct tgacgacctc
     7141 gacggtgggg ggcttggcat aagcgttcgc gatcgcgggg acgccggccg aatttgtgtc
     7201 gccatcgtgc agaaccactc ggtagctgaa ttcaatggct tgcccatctt caatcatgta
     7261 attccctttg tccggtcggc caaaatcgtg ccagccgaag ggattggccg cgaacagccc
     7321 gtacgtgcgg acgtgccagg tggtcgggta ccgaaaactg gtgggatggt tgatgatggc
     7381 aatcccgacc gtacggtctt tgaccgggcc gacgtagtca acccacggtg agggcttgcc
     7441 ccatgctttg tcgtctgtca aaccttccgc gttcgtgatc ttaccccccg ttttcttgtc
     7501 aacgtccatc gacgacgcga cgcgaatgcc aaacatgcct tccttggtgt cacggaactc
     7561 caccggccct gcgctagcga tgatccggac cttgaagtcg atcaaccgcg cggacttcgt
     7621 gcgataaaat gtgaccaggc gctcgtcggt gcagaccttc ttgtcgtcgg ccgcgcgcca
     7681 ctcgttcagg gttttcaggc gtccgatgac ggggcccgcg acgaccaaag tccgctcgtt
     7741 ctccttgacg gttccaaacc gcttgccctc cgaccaaaaa tcaatgccgt taacattacc
     7801 gaaagtgaac cagcacgacc gttggtgagg atgatcgtga tcttcgtcgg cacggatcga
     7861 caagggatac tcacgcgtgt aggactcgcc ggtcggtc
//
LOCUS       DATAQL010000655         6007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1747845, whole genome shotgun sequence.
ACCESSION   DATAQL010000655 DATAQL010000000
VERSION     DATAQL010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747845"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VHS97_11430"
     CDS             <1..357
                     /locus_tag="VHS97_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3448859.1"
                     /translation="GTCAHCMQQLELFGKEYEALKKMDVETVAISSDDVSAARSLKNN
                     KDGVKFPMPMLADPKLELFKLYRAFDDFENQPLHATFLIDAQGNVRYQRISADPFLDV
                     EFIKTEAGRINRLLKR"
     gene            663..1445
                     /locus_tag="VHS97_11435"
     CDS             663..1445
                     /locus_tag="VHS97_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX3448860.1"
                     /translation="MSAGSLLQARQNESNSRLAAPADPGLPLVSLPARHRHRLQPLDN
                     KPEGCLLIHEIYRSLQGESTFAGLPCVFIRLTACNLRCVYCDTPHAFTEGRIVDLDAV
                     VAKGLELGDDLVEITGGEPLLQPEVYPLMTRLADAGRTVLLETSGAIDTSAVDPRVRV
                     ILDIKTPGSGEVDANVWTNIDRLKPTDEVKVVVCDRADFDWAAAIVRTQGLARRCPVL
                     FSASFGQVDATELAGWILETRLPIRLQLQQHKILWNPSARGV"
     gene            complement(1473..1706)
                     /locus_tag="VHS97_11440"
     CDS             complement(1473..1706)
                     /locus_tag="VHS97_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448861.1"
                     /translation="MGPRSLVREAWEMIPILLLLMVGGMIALKSGVRRSGESQNLRQV
                     VGNLSQMTLRVLGYVAVLLALQYWIGLRPQLGW"
     gene            complement(1965..2789)
                     /locus_tag="VHS97_11445"
     CDS             complement(1965..2789)
                     /locus_tag="VHS97_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX3448862.1"
                     /translation="MNPVAQQPSMLVDTHAHLDDPRLRANLDDLLKRASAAGVRQIIA
                     IGTTAATSTDCVEIAGAYRGVFAAVGIHPNEAAEAGENDWRAITDLVERPGAVAVGET
                     GLDGYWDSTPFALQQEWFDRHLNLARERDLSIVIHCRDCMGEIIDQMQRLVRRLGRPI
                     RGVMHSFTGSWEDAESCLELGLHLSFAGMVTFTNKTLDPLREVAARVPLDRLLVETDS
                     PYLTPHPHRGQTNEPARVALTAARLAEIRGMTFTQFAQITTANARSLFSLPETNVL"
     gene            complement(2786..3334)
                     /locus_tag="VHS97_11450"
     CDS             complement(2786..3334)
                     /locus_tag="VHS97_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX3448863.1"
                     /translation="METLPVDQVRLRAIIENAGLRCTPQRLAVYQQLCESDEHPSAEI
                     VFHAVRAQIPRISLATVYKALEALVAVGVATKLSAGIGGTRARYDARRDDHYHFRCLR
                     SGTVHDLPTQFDPDLIAKLDPQLQDDLCRQGFRVTGYRLELVGYQERTILDSSESPPN
                     GGEDLADSIQSAYPPNQGAGNP"
     gene            3757..5466
                     /gene="nusA"
                     /locus_tag="VHS97_11455"
     CDS             3757..5466
                     /gene="nusA"
                     /locus_tag="VHS97_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX3448864.1"
                     /translation="MSVDLVRIVDSIHRDKNISKDILFEGIQSALTTAARKHYPDAGE
                     IEVRIDPDSGAIDATKDGVKMDPAELGRIAAQTAKQVIIQKIREAERDSLFDEFEDQR
                     GDLVTGTVQRFEGGAVIVNLGKTDAILPRSDQIPGESYHPNERIRAIILDVRKVGQRV
                     KIILSRTHSDFVRRLFELEIPEIADQIIAIRALAREAGYRSKVAVTSIDTKVDAVGAC
                     VGVRGTRIKNIVDELGGERIDIVRWNESLQVLIPNALQPAEIDEVMLCHLLGRAIVLV
                     RDDQLSLAIGRRGQNVRLASKLVGWDIEIMTAEELDEVIEKAVKAFEKIEVVDTELAE
                     RLVEQGILSYDDLSVMEISDLVNTIEGLTEEQAVEIVAKAEVLAEAQTEELPRRKGAR
                     ASASEPAPPVETLEEGESESDTDTEVSADGSAAADEFTETDSESSINDAATLESSSAL
                     PSERVNEVAGEFPSDDSTDSERDEASDDEIHDLALAEEKSNYSPHGHEVSSPPSDEDQ
                     GESIRIATEAVEQSGPQAPAGAPPAKNREPAASPRHSTPPPSTSLSNHRSDGALGHAD
                     DES"
     gene            5596..>6007
                     /locus_tag="VHS97_11460"
     CDS             5596..>6007
                     /locus_tag="VHS97_11460"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX3448865.1"
                     /translation="MSTRVHELAKELGLKSQELLERIQKWGLDVKANALASLDPEMVD
                     RIRELMDQPAALKGSATATAASAAKPAAAPSSLAASATGAPRASAGEERPAPSTRPAG
                     PAPIAAGPAKIEPVRAAEAAPAAVSSPVPGTAPPQ"
BASE COUNT         1203 a         1788 c         1887 g         1129 t
ORIGIN      
        1 gggacgtgcg cccactgcat gcagcagctt gagctgttcg gcaaggaata cgaggcgctc
       61 aagaaaatgg acgtcgagac agtcgccatc agcagcgacg acgtctccgc cgcccggtcg
      121 ttaaagaaca acaaagacgg cgtcaaattc cccatgccca tgctagcgga ccccaagctc
      181 gagcttttca aactttatcg cgcgttcgac gatttcgaga accaaccgct ccacgcgacg
      241 ttcctgatcg acgcccaggg gaatgtccgg taccagcgga tctcggccga tcccttcctc
      301 gatgtcgagt tcatcaagac cgaggcaggg cggatcaatc ggctgctaaa gcgatgatga
      361 tttgcgagat gtggaaaggg agcgggtgcg atgcctctgc tcgacttgaa ttttggccgt
      421 caacggtaga aattggcaag aacttacctt tgcttcctct agcacgcaat tccgagtgat
      481 gtcttcccat cgatccggaa gggacctacg ctgccgcgtg tccgcggcga ccgctggcat
      541 gagccagtta cgcggcagca tcggatgggc gcggccggat tgaaaagcct gctctgggcg
      601 agctggtatt cactccgtat gatgaatgca gatagcaaat tgtgactttc gatggagtgg
      661 ccatgtctgc ggggtcgttg cttcaagctc gtcaaaacga atcgaattcg cggttggctg
      721 caccggccga tcccggattg ccactggtct cactccccgc gcgtcatcgc caccggcttc
      781 agccgctcga taataagccc gaaggctgcc tgctcatcca tgagatctac cgcagcctgc
      841 agggggaaag tacattcgcg ggcttgcctt gcgtttttat ccgtctgacc gcctgcaacc
      901 tgcgatgtgt ctactgcgac accccccatg cctttacaga gggtcgaata gtcgacctgg
      961 acgcggtcgt ggccaagggt cttgagctgg gcgacgactt ggtcgaaatc acggggggcg
     1021 agcccctgtt gcagccggag gtatatccgc tcatgacgcg gctagccgat gccggccgga
     1081 ctgtcttgct tgagacgagc ggcgcgatcg acacgagcgc cgttgacccc cgcgtgcgtg
     1141 tgatccttga tatcaagacg cctggttccg gcgaagtcga cgccaatgtc tggaccaaca
     1201 tcgatcggct caagccgacg gatgaggtca aggtcgtcgt ctgcgatcga gccgacttcg
     1261 actgggcagc agcgatcgtg cgcacgcaag gattggcgcg gcgatgcccg gtcttgttca
     1321 gtgcatcgtt tgggcaggtg gacgccaccg agctggcggg ctggattctt gaaacccgtc
     1381 tgccgatccg gcttcagctc cagcagcata agatcctttg gaatccgagc gcccgtggcg
     1441 tctgacagcc cgccatgttt cctgtgatgg gattaccatc ccagttgggg gcgcaagccg
     1501 atccagtact ggagcgccag cagcacggcc acatagccca ggactcgaag cgtcatctgc
     1561 gaaaggttgc cgacaacctg ccgaaggttc tggctttcgc ccgaccgacg aaccccactt
     1621 ttgagcgcga tcatgccgcc caccatgagg agcagcagga tcggaatcat ttcccaagcc
     1681 tctcgcacca gactccgcgg acccatgatc gaacggtctc ccgatggtgt ttggcgatgc
     1741 agaaagacaa acgggttgat cagactcgcc gcgccctatg ccaaataatg agccgtccga
     1801 tcggcgggca ttgttcgttt cggttctgaa ttaaaagcta caagacgaca atcgtcgagg
     1861 cgaaaagtgg ttttttgaag gctttgcaat cggtcgaagt gagagtatgc gggtcgcgca
     1921 aagagcgcgg gctctaacga ttgggcttgt tgagcctggg tcgctcagag gacgttggtc
     1981 tcaggaagac taaacaggct gcgcgcattg gctgttgtga tctgggcaaa ctgggtgaac
     2041 gtcatgcccc gaatttccgc cagccgcgcg gccgtcaagg ccacacgggc cggttcgttc
     2101 gtttgtcctc ggtgggggtg tggggtgagg tagggactgt ccgtttcgac aaggaggcga
     2161 tcgagaggaa cgcgggcggc gacctcgcgc agtgggtcga gcgttttgtt ggtgaaggtg
     2221 accataccgg cgaaagacag gtgtaaccct aactcgagac aggattcggc gtcttcccac
     2281 gacccggtaa acgagtgcat cacccctcga atgggacggc ccagacgtcg cacgagacgt
     2341 tgcatttgat cgatgatctc gcccatgcag tctcggcaat gaatgactat agaaaggtcc
     2401 cgttcgcggg ccaagttcag atggcggtcg aaccactctt gctgcagcgc aaacggggtg
     2461 ctatcccagt atccatccaa tcccgtttca cccaccgcaa cggcgcctgg ccgctctacc
     2521 aggtcggtga tcgcgcgcca gtcattttcg cccgcttcgg cagcctcgtt ggggtggatt
     2581 ccgaccgcgg caaagacacc gcggtatgcc ccagcaatct caacacagtc agtactcgtc
     2641 gccgcggtgg tgccgatggc gatgatttga cggacgccgg ccgcactggc ccgtttgagc
     2701 agatcatcca ggttggcgcg gagtcgcggg tcgtccagat gcgcgtgggt atccacaagc
     2761 atggacggtt gttgcgccac ggggttcatg ggttgcccgc gccttggttc ggcggatagg
     2821 ccgattggat ggaatcggcc agatcctcgc ccccgttggg gggcgattcc gaactgtcaa
     2881 gaatggtgcg ctcttggtac ccgaccaatt ccagtcgata gcctgtgacc cgaaaaccct
     2941 gacgacagag gtcatcttgc aactgcgggt cgagtttggc gattaggtcg gggtcgaatt
     3001 gcgtcggcaa gtcgtgaacg gtcccggagc gcaggcaacg gaagtggtag tggtcgtcgc
     3061 ggcgtgcatc gtaccgcgcg cgggtaccgc cgatgcccgc gctcagcttg gtcgcgactc
     3121 cgacagccac caaggcttcg agtgccttgt aaacggtcgc cagactgatt ctgggaatct
     3181 gggcgcggac ggcgtgaaag acgatttccg ccgaaggatg ttcgtcggac tcgcagagtt
     3241 gctggtacac cgcgagccgt tgcggagtgc accgcaaacc ggcgttctcg attattgcac
     3301 gaagccgtac ctggtcgacc ggcaaggttt ccatcactgt atctggttcc cccgcccgcc
     3361 taccacgacc ctgtcgcggc cgatctggga gttggagttg gtgcggccga gcccggccgc
     3421 aattccttca tggcaatcgc ctttctattg ttcggcggct tgacagccgt gtcaacggcg
     3481 tgttaaattc attgcttggg ccggggctgg gaaggcagat catcgggctc ggcccttcag
     3541 gggcttggtt cgctccctgc gcccagaatc ccgtgcggcc tgggccatcg agtgctgcca
     3601 cggaggtgtt gctcgtttcg gcgcaccgcg ataggaattc cgagccccta ccgagtgggg
     3661 cggcgttcct gcggctgccg cctgctccgc ttgggcccgg cgtcccgctc cgccgggtag
     3721 cggtcaatcc cattgagcac gcctcggagg agatgaatga gcgtcgattt ggttcgcatt
     3781 gtcgatagca tccatcgcga caagaatatc tccaaggata ttctgttcga ggggatccag
     3841 tcggcactca ccaccgcggc acgcaagcat tatcccgacg cgggtgagat cgaagtccga
     3901 atcgatcccg attcgggcgc catcgacgcg accaaggatg gcgtcaagat ggaccccgcc
     3961 gagctgggac gaatcgccgc gcagacggcc aagcaggtga tcattcagaa gattcgggag
     4021 gctgagcgcg atagcctttt cgacgaattc gaagatcagc gcggcgacct ggtcaccggg
     4081 actgtgcagc gattcgaggg tggcgcggtc attgtcaatc tgggcaagac cgacgcgatc
     4141 ttgccgcgaa gcgatcagat cccgggtgag agctaccacc ccaatgagcg aatccgcgcg
     4201 atcatccttg atgtccgcaa agtcggccag cgggtcaaga tcattttaag ccgcacccac
     4261 tccgactttg ttcgccgcct tttcgagctg gagattcccg agatcgccga tcagatcatc
     4321 gctatccgcg cgctggctcg cgaagccggc tatcgctcca aggtcgcggt gacctcgatc
     4381 gataccaagg tcgacgccgt cggcgcctgt gtgggtgtgc gcgggacgcg gatcaaaaac
     4441 atcgtcgacg agctcggtgg cgagcgaata gacatcgtgc ggtggaacga atcgctgcag
     4501 gtcttgattc ccaatgcgct tcagcccgcc gaaatcgacg aagtcatgct ctgtcacttg
     4561 ctgggccgcg cgatcgttct cgtccgggac gaccaactct cgctggccat cggccgccga
     4621 ggccagaacg ttcggctcgc ctccaagctg gtcggctggg acatcgagat catgaccgcc
     4681 gaagagctcg acgaggtgat cgaaaaggcg gtcaaagcct tcgagaaaat cgaggtcgtc
     4741 gacaccgaat tggccgaacg tttggtggaa cagggcatcc tgagctatga cgacctgtcc
     4801 gtgatggaaa tctcggattt agtgaacaca attgaaggat tgaccgaaga acaggcagtc
     4861 gaaatcgtgg ccaaggcgga ggttctggcc gaggcccaga ccgaggagct tccccgccgc
     4921 aagggagcgc gggcctccgc gagcgagccg gctccccctg tagaaaccct cgaagaggga
     4981 gagagcgaaa gcgacaccga caccgaagtc tcggccgatg ggtcagccgc ggccgatgag
     5041 tttacagaga ccgattcaga atcaagtatc aacgatgccg cgaccctgga atccagctcg
     5101 gccttgccct ccgagcgtgt taacgaggtc gcgggcgaat tcccttccga cgacagcacg
     5161 gattcggagc gtgacgaggc cagcgacgac gagatccacg acttggccct ggcggaggag
     5221 aaatccaatt actcgcccca cggtcatgag gtgtcttccc cgcccagcga cgaagatcag
     5281 ggcgaaagca ttcgaatcgc gaccgaagcg gtcgaacaga gtggtccgca agccccggcg
     5341 ggagcgccgc cggccaagaa ccgcgaaccg gccgcgtctc cgcgtcattc cacgccgccg
     5401 ccgtcgacct cgttatcgaa ccaccgttcg gatggggccc tcggtcatgc tgacgacgaa
     5461 tcctaaagct aaagtaccgt ggcgaccgct agccgggccg cgataggaac agaacagatc
     5521 aacccgaaca atagagaata ccctccggtc gcggctggcc tgggaaaccg gagtcaaatc
     5581 cgggaggagt tgcctttgtc gacgcgcgtt catgagttgg cgaaagaact aggcttaaag
     5641 agccaagaat tgctcgagcg catccagaag tggggtctcg acgtcaaggc aaacgccctg
     5701 gcgagtctcg acccggagat ggtggatcgg attcgggagt tgatggacca gccagcggct
     5761 cttaaggggt cggcaaccgc aaccgcggcc tcggccgcca aaccagcggc cgcgccgtcg
     5821 tcgttggcgg catcggccac gggtgctccc cgtgcgagcg cgggcgaaga acggccagca
     5881 ccctcgacgc ggccggcagg gcctgcaccg attgcggcgg gtccggccaa gatcgagccg
     5941 gtcagggcgg cggaggccgc ccccgcggct gtaagctcac cggtcccggg cacggcgcct
     6001 cctcaac
//
LOCUS       DATAQL010000656         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4647400, whole genome shotgun sequence.
ACCESSION   DATAQL010000656 DATAQL010000000
VERSION     DATAQL010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4647400"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VHS97_11465"
     CDS             <1..332
                     /locus_tag="VHS97_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448866.1"
                     /translation="NFLDPSDGLLDDFLELAVEGERVGKRPYGCPLFNVHIGDGHFSA
                     LGSRSWARIVGRRLELLLERTQSLDNVIRSSEAPPRDDASLGQTNTPSEPRHTGTTKP
                     DLAGEER"
     gene            336..1742
                     /locus_tag="VHS97_11470"
     CDS             336..1742
                     /locus_tag="VHS97_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3448867.1"
                     /translation="MISWRRKIARSFRSRRPEKRQRVFAFVSRAERLERTFKLTIAIT
                     TMFLLASFLTFLSPGRYLTSWLKARAWQLTTQAMGLPLDRGEIDADWQRKRLFDIEQS
                     RGTLEGTFAEYPPAMQRLLRYAGLDPEHALVRWGNFDRTVLLPSTVFEAEQSGRSYQF
                     RPNVRSIWIRNFPVKGPVKAYFQVPESPEIIDLVKGTGAEIVSGSTQTTNSWALRGPE
                     PDLKAPWRGIVLGDSYMQGLFIGDDQTPTECLKRELTTRLGATVEVLNTGHLGYSPEQ
                     YYYTLVEYGKRFAPHFVVVSFFANDFGDFQEALEGKGDWEEAQYWMSRISQYCFSHPA
                     ECVMVPAPWVNQIEGPQRAGNYPGKLANILDVAGPGYLDPMPAFANAQLEISNKARRL
                     NISSEASPLFNGRIGDGHFSARGSEVWASAVGRRVALLLQRRRLAEEHVPRHTPTNDK
                     RQVDISPNDRAEFTRRSP"
     gene            complement(1723..2145)
                     /locus_tag="VHS97_11475"
     CDS             complement(1723..2145)
                     /locus_tag="VHS97_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX3448868.1"
                     /translation="MKSHTEYLTFNVPARMDFINITSQVEDIVKKSGIQEGLLLCNAM
                     HITASVFINDDEPGLHDDYKRWLEQLAPFDPSPTRYKHNRTGEDNADAHHKRQVMGRE
                     VVVAVTKGKLDFGPWEQIFYGEFDGRRSKRVLVKVIGE"
BASE COUNT          525 a          743 c          749 g          515 t
ORIGIN      
        1 tcaattttct cgacccgagc gacggtctac tggacgattt tcttgagcta gccgtcgaag
       61 gagaacgagt cggcaagagg ccgtacggtt gccctctatt caacgtccac atcggcgatg
      121 gtcacttctc tgcgctggga tctcgatcct gggcgaggat tgtggggagg cggctggaac
      181 tcttgctgga aagaacgcaa tcgctcgaca acgtgattcg ctcgagtgaa gccccgcctc
      241 gcgacgatgc aagccttggc cagacaaaca ctccgagcga gccccggcat accggcacga
      301 ccaagcccga tctagcggga gaggaacgct agcgaatgat ctcttggcga cgaaagatcg
      361 cgcggtcgtt tcgatcgcgt cgacccgaaa aacgacagcg tgtgttcgcg ttcgtttcgc
      421 gggccgagcg gctcgagcgc accttcaaat taacaatcgc tattacaacc atgttcttgc
      481 tcgcttcgtt tctgacattt ttgtcgcccg gccgctacct gacgagttgg ctcaaagcgc
      541 gggcgtggca gctcacgacg caagcgatgg gcttgccgct ggatcgaggt gaaatcgacg
      601 ccgactggca acgcaagcgg ctcttcgaca tcgaacaatc gcgcggcacc ttagagggta
      661 cgtttgcgga ataccctcca gcgatgcagc ggctcctgcg ctacgccggg ctcgatcccg
      721 agcacgccct ggtacgctgg ggtaatttcg accggacggt tctcttgccg tcgaccgtgt
      781 tcgaggccga gcagtcgggt cggtcgtacc aattccgacc caatgtgcgc tcgatctgga
      841 tccgcaattt tccggtcaag ggaccggtaa aggcctactt tcaagtgccc gagagccccg
      901 agatcatcga cctcgtcaag ggaacgggcg ccgagattgt gagcggatcg acccaaacga
      961 ccaactcatg ggccttacgt ggaccggagc ccgatctcaa ggcaccatgg cgcgggatcg
     1021 tgctggggga ttcttatatg caaggtctct tcatcggcga tgaccagact ccgaccgagt
     1081 gcctgaagcg cgagctgacg acccggctgg gagccacggt cgaagtcttg aacaccggac
     1141 acctcggcta ctcgccggag cagtactact acacgttggt cgagtacggc aagcggtttg
     1201 ccccccattt cgtggtggtg agctttttcg ccaacgattt cggcgatttc caggaggcac
     1261 tcgaaggcaa gggtgactgg gaagaggcac aatactggat gagccgaatc tcgcagtatt
     1321 gtttctcgca ccccgccgaa tgtgtcatgg tccccgcccc ttgggtcaac cagatcgagg
     1381 ggccgcagag ggcgggcaat tatccgggca agctggccaa cattctcgac gtggccgggc
     1441 ccggctatct cgacccgatg cccgcatttg ctaatgccca actcgagatc tcgaacaagg
     1501 ctcgccggct caatatatct tcggaagcca gcccgctttt taacggccga atcggcgacg
     1561 gccatttctc ggcgcgcgga tcagaggtct gggccagcgc tgtcggccgc agggtcgcac
     1621 tgctgctaca gcgccgccgg ctggccgaag aacacgtgcc acggcacact cccactaatg
     1681 acaaacggca ggtcgatatt tcgccaaacg accgcgccga gtttactcgc cgatcacctt
     1741 gaccaaaacg cgcttggatc tgcgtccgtc gaattcgccg taaaagatct gctcccaggg
     1801 accgaaatcg agctttccct tggtaaccgc cacgaccacc tcccgaccca tgacctggcg
     1861 tttatggtga gcgtcggcgt tgtcttcgcc agtgcggttg tgcttgtaac gtgtggggga
     1921 agggtcaaag ggcgcaagct gttccagcca ccgcttgtaa tcgtcgtgca ggccaggctc
     1981 atcgtcgtta atgaatacgc tggccgtgat gtgcatggcg ttgcagagga gtagaccctc
     2041 ttgaattccg ctcttcttga cgatgtcttc gacctggctc gtgatgttga taaagtccat
     2101 ccgggccggc acgttgaagg tcaagtattc ggtatgcgat ttcatgatga cacgaccacc
     2161 ggtccgacgc ctgggccggc cgcgcgatca cgcggccgac ccgaaaatcg taattgattc
     2221 acgacaccgg aaacatggcc agggcctgct tggcgatgac gattcgttta agtcacataa
     2281 atctattcag accaagccgg aaggtcaact gcggaagttc atgacagtaa acacagatcg
     2341 acctctggtt gtcattcgct gagccgatcg caacctcgtt ttcgtcatgc aaaaaaggac
     2401 ggcgcggcgc gtccgtgtga tggatgcaga acacggacaa ggggcgagcc gagttcgata
     2461 atcgggattt ggcgaccgta aaccgagggt ggttagcccg attcagggat cgccggccag
     2521 tggctctggt tc
//
LOCUS       DATAQL010000657         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3886064, whole genome shotgun sequence.
ACCESSION   DATAQL010000657 DATAQL010000000
VERSION     DATAQL010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3886064"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VHS97_11480"
     CDS             <1..308
                     /locus_tag="VHS97_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448869.1"
                     /translation="LGGASALITTTIQLPNTTTNTVTTTVTVPDGGTVLLGGVKTLTE
                     TRTENGVPILSKVPMIDRLFRNVGIGRATTSLMLMVTPRIIILEEEEEALGIPSVAF"
     gene            540..1265
                     /locus_tag="VHS97_11485"
     CDS             540..1265
                     /locus_tag="VHS97_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HEX3448870.1"
                     /translation="MFPGLTLVYVLVLCSGPVADDGGKQAVEVLQPDPAWKKMGRNLW
                     FDPKAKKVILRARVVLREGYLEHFMCSKGTKEHEAILATDAVPHQIHTTLILTGAEAG
                     HPVRFVPKFEPPSGTVIAIDVEWRQDGKVHKSDARQWIKDEKAKAPLAIDWVFAGSEF
                     FVDPATNKQAYAADEGDLITVANFGSAILDLPIASSASDADRVYVTNTEKIPPLGTEV
                     FVTLGPRAAKASDKRGSGVTPSR"
     gene            1262..2632
                     /gene="glp"
                     /locus_tag="VHS97_11490"
     CDS             1262..2632
                     /gene="glp"
                     /locus_tag="VHS97_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX3448871.1"
                     /translation="MNGDTISKPGLQGDPRMKGFRSRTSVAALAAWIDETIEALPSEG
                     VSLEAAWGRVLADDVIAPDAVPPFDRCAMDGYAVRAEETFGASDYMPSVFRRVGRSRP
                     GVPCQAAVGPSEAVEVATGSPLPTGADAVVPVEATRLDGDMVLVAEAVPQGRHVSRRG
                     EDIVPGTVVLNAGRVLRPQDLGVLSAVGAAKVAVVRSPRVVVLITGDELLPPGTPSRG
                     VQIADANSVMLRALVTRDGGCCVVDGPLADRRAGIREAILAAAGRADLILITGGSSAG
                     ADDHVPGIITELGCQIAHGLALRPAGPTGVGLILEQELPVVMLPGNPVSCLCGYDFVA
                     GPIVRRLAGRPGQWPYRRVTLPLARKLSSVVGRVDYARVRVCSGQVEPLSSSGASILT
                     SVSHASGFVVIAEGREGYPAGAMVDVWCYDDFATHENSSTGNPIDPSRESRDQSTRSA
                     HQTPAG"
     gene            2794..>3004
                     /locus_tag="VHS97_11495"
     CDS             2794..>3004
                     /locus_tag="VHS97_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3448872.1"
                     /translation="MENGALIVTEGLTKVYRRVTALEGLSLQVGRGEVYGLLGPNGSG
                     KTTTIRLLLGLLRPTAGRATVAGFDS"
BASE COUNT          564 a          891 c          962 g          587 t
ORIGIN      
        1 gtcttggcgg tgcatccgct ctgatcacaa ccacaattca gctccccaat accaccacca
       61 acacggtaac gacgaccgta acggttcccg atggcggtac ggttctctta ggcggtgtca
      121 aaacactcac cgagacccgt acggagaatg gcgtgcccat tctgtccaag gtaccgatga
      181 tcgatcgtct gttccgcaac gtcggcatcg gtcgcgccac caccagcttg atgctcatgg
      241 tgacgccgcg aatcatcatt ctcgaggaag aagaggaagc actcggcatc ccctcggtcg
      301 cgttctgacc ctcgcgtagg ccgcgggatc agatcgactc ggggacatac aaaaatctcg
      361 aaggccgccg gaagttgctt ccggcggcct ttttttgcgc gcattggcca ccttcgcgat
      421 gcggcgccgc ccgtacgatc ccaggtcggc gccggcgcag tccttgatct gctgctgggt
      481 tttgaggatg ctaaacgagg taaaccgcaa tgttctgacg ccggacaagc gagacaacca
      541 tgttcccagg gctgactctg gtttatgtgc tcgttctctg cagtgggcct gttgccgacg
      601 atggcggcaa gcaggctgtg gaagtactcc agcctgatcc cgcctggaaa aaaatggggc
      661 gcaatctctg gttcgacccc aaggcgaaga aggtgatctt gcgagcgcgg gtggtgttgc
      721 gggaaggtta tctcgaacat ttcatgtgtt ccaaggggac caaggagcac gaagcaatcc
      781 tggcgacgga tgccgttcct caccagattc ataccacttt aatcctgaca ggcgccgagg
      841 ccggtcatcc tgtacggttc gttcccaaat tcgaaccacc atccggaaca gtaatcgcga
      901 tcgacgtcga gtggcgacaa gacggcaagg tccacaagtc ggacgctcgc cagtggatca
      961 aggacgaaaa ggcgaaggct cccctggcga tcgactgggt gttcgccggc agcgagttct
     1021 tcgtcgaccc agccaccaac aagcaggcct acgccgccga cgaaggagac ttgatcacgg
     1081 tggcgaattt tggcagcgcg attcttgacc tgccaattgc cagttccgcc agtgacgcag
     1141 accgagtgta cgttacaaac accgagaaaa tccctccgct tggtaccgag gtttttgtca
     1201 cgcttggtcc ccgcgcagcc aaggcaagcg acaaacgagg ttccggcgtg acgccaagtc
     1261 gatgaacggc gacacgatct cgaagcctgg cctgcagggc gatccccgaa tgaagggatt
     1321 ccggtcgcgc acgagcgtcg ctgccctcgc cgcctggatt gatgagacga tcgaggcgct
     1381 gccgagtgaa ggtgtatcgc tcgaggcggc gtggggccgg gtcttggccg atgacgtcat
     1441 cgcgcctgac gcggtgccac cgttcgatcg atgcgcgatg gatggctacg ccgtccgggc
     1501 cgaagaaact ttcggcgcat cggattacat gccgtcggtc ttccgtcgcg tgggcaggtc
     1561 gcgcccagga gtgccctgtc aagccgccgt cggcccgtcg gaagcggtcg aggtcgccac
     1621 gggatctcct ctgcccacgg gggcggatgc cgtcgtcccg gtcgaggcca ctcgactcga
     1681 cggcgacatg gtcttggtgg ccgaggctgt cccccaggga cgacatgtga gtcggcgcgg
     1741 agaggacatt gttcctggca ctgtcgtgct gaatgccggc cgcgtgctcc ggcctcagga
     1801 cctgggggtt ttgagcgccg tcggagccgc caaagtggcc gtggtccgaa gtcccagggt
     1861 ggttgtgctg atcaccggtg acgagctgct cccccctgga acgccgtcgc gtggggttca
     1921 gatcgccgat gccaattctg tgatgctcag agccctcgta actcgggatg gaggttgttg
     1981 cgtggtcgat ggcccgttgg ccgatcgtcg agcggggatc cgtgaggcca tactcgccgc
     2041 cgccggccga gccgatctga tactgataac tggcggaagc tcggcggggg ccgacgatca
     2101 cgtgccgggg atcatcaccg agctcggctg ccagatcgcg cacggcttgg ccctgcgccc
     2161 ggcgggtccg accggagttg gcctgatcct cgagcaagag ctgcccgtag taatgctgcc
     2221 gggtaacccc gtgagctgcc tgtgcggcta cgacttcgtc gccggaccga tcgtccgcag
     2281 gctcgctggc cgaccgggac agtggcccta ccgccgcgtc actctgccgc tcgcccgcaa
     2341 gctgagttcg gtcgtgggcc gggtcgacta tgcgcgggta cgagtttgct cgggccaggt
     2401 cgagccgctc tcctcgagcg gggcgtcgat tctgacgagc gtgtcgcacg caagcgggtt
     2461 tgtcgtgatt gccgagggaa gggaaggcta tcccgccggc gcaatggtcg acgtgtggtg
     2521 ctacgacgac tttgcaaccc atgaaaatag ctccaccggt aatccgattg atccatcacg
     2581 ggaaagccgc gatcagtcca cccgcagcgc gcaccaaacc cctgccggat gaaattgggc
     2641 atcagcagga ggtgtctcca tcctttccca ttgttccgaa gtccagatga tgagcctcgc
     2701 tcgatatccg ttgattcgcc aatttgacgt tatggcaaca tttcttgggc agcggagtac
     2761 atcttcattt tgaagggaga ctgaataagg gacatggaaa atggcgcttt gatcgttacc
     2821 gagggtctta ccaaggtgta caggcgggta actgcactcg agggtttgtc gctgcaggtt
     2881 ggccgtggag aggtgtacgg cctactgggc ccgaatggtt cggggaagac gacgacgatt
     2941 cgactgttgc tggggctctt gcgaccgacg gcaggtcggg caacggtagc gggattcgac
     3001 tcgt
//
LOCUS       DATAQL010000658         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_679312, whole genome shotgun sequence.
ACCESSION   DATAQL010000658 DATAQL010000000
VERSION     DATAQL010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679312"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VHS97_11500"
     CDS             complement(<1..302)
                     /locus_tag="VHS97_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448873.1"
                     /translation="MRRLLPALLLLVTALPAWGHPIPSSAVVLRLHRTGIDAELTLPI
                     GERAMGWEKPLPLDSVKTVQKYGAELEAYVRTHVRPSAPDGRPWIVTVRGVTPLDE"
     gene            complement(310..1470)
                     /locus_tag="VHS97_11505"
     CDS             complement(310..1470)
                     /locus_tag="VHS97_11505"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX3448874.1"
                     /translation="MRHGVSARTVLALTAAAGTLSLAIFATNVASFVPQAVAQTPTPR
                     PDLLASPKADDVVDAGEAFLATLSEEQRATAQIELTPQLAVRWTNFPGGSNVRNGVFF
                     RDLKPAQVEAALKVARIALGEEGFSRYQEVRAADDAFAKGRGGRGPGGPDQKKADGDP
                     AKKKAGGVAKKKGGGGGMNFGAMNYMIAFLGKPSKTTPWILQLGGHHLAINIYYKGTT
                     GGATPYHVAAQPTVWKDDQGKTHEPLAPMRESLHGLLLSLTPEQSKQAKLEARFNDVY
                     VGPGKDGKFPAKSEGVPVSELADASKDFVKKAIAAWTGDSPQGAEYRKLYEAELEKVK
                     VSYSGTTNLNDRGDYVRIDGPRVWIEFATQGSDHVHTIWRDRLTDYGAEFSF"
     gene            complement(1585..1722)
                     /locus_tag="VHS97_11510"
     CDS             complement(1585..1722)
                     /locus_tag="VHS97_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448875.1"
                     /translation="MRYYLCTRYLSAKRFGEAVRGRWGIESTRRVLDVNMKGILVFLA
                     A"
     gene            1866..2507
                     /locus_tag="VHS97_11515"
     CDS             1866..2507
                     /locus_tag="VHS97_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3448876.1"
                     /translation="MELPDWFLTPLWRAAHDGRLASVRLLVERGANVNHRNPDGSHQA
                     LKTAAENDRLETCAYLIDHGATPDLITAAMLGLDTRVRELLEESLEAMSARDEHGRSA
                     LDAATLLDSFRQCRDGLHHGHDRVAHLLIEHGSTVELEHAASLGLCAELRRMVELDPD
                     ILKRPKVMKALMGGTATKESPLQAARRRGRTEIVSYLLEHGAVDVPAVIIVET"
BASE COUNT          453 a          781 c          794 g          500 t
ORIGIN      
        1 tgctcatcaa gcggggtcac tccgcgcacc gtcacgatcc aaggccgccc gtctggagca
       61 gatgggcgga catgtgtgcg gacatatgct tcgagttctg ctccatattt ctgcaccgtc
      121 ttgacagagt ccaacggcaa cggcttctcc cagcccatcg cccgttcgcc gatcggcagc
      181 gtcaattcgg cgtcgatacc ggtgcgatgg agacgcaaca caaccgccga actggggatg
      241 ggatggcccc atgccgggag cgctgtcact agcagtagga gggccgggag gagtcgtcgc
      301 attgatgcct cagaaggaga attctgcgcc gtagtcggtc agccggtccc gccagatcgt
      361 gtggacatgg tcgctaccct gcgtggcgaa ttcaatccag acgcgtggcc cgtcgatgcg
      421 cacgtagtcg ccccgatcgt tcaggttcgt cgtgccggag taggacacct tgactttttc
      481 gagttcagcc tcgtagagct tgcggtattc tgcgccctgc gggctatcac ccgtccaggc
      541 cgcgattgct ttcttgacga aatccttcga ggcatcggca agctcggaga ccggaactcc
      601 ctcgctcttg gcagggaact tgccgtcctt gccgggaccg acgtagacat cgttgaaacg
      661 cgcttcgagt ttcgcctgtt tcgactgctc gggtgtgagg gagagaagca agccgtgcag
      721 tgattcacgc atcggggcga gcggttcgtg cgtcttgccc tgatcgtcct tccacaccgt
      781 cggctgagcg gcgacgtggt aaggggtcgc gccgccggtt gttcccttgt agtagatgtt
      841 gatggcgaga tggtggccgc ccaactgcag gatccagggc gtcgtcttcg agggcttgcc
      901 caggaacgcg atcatgtagt tcatcgcccc gaaattcatc cccccacctc cgcccttctt
      961 cttggcgact ccgccggctt tcttcttggc cggatcgcca tcggctttct tctgatcagg
     1021 accgccgggc cctcggccgc cacgcccttt ggcgaacgcg tcatcggccg cgcggacttc
     1081 ctggtatcgt gagaaaccct cctcgccgag ggcaatccgg gcgaccttca gggccgcctc
     1141 gacctgcgcg ggcttaaggt cgcggaagaa gacgccgttg cgcacattgg acccacccgg
     1201 aaagttcgtc caccggacgg cgagttgcgg cgtgagttcg atctgggcgg tcgctcgctg
     1261 ctcctccgac agcgtggcca ggaaagcctc tccggcgtct acgacgtcgt ccgccttcgg
     1321 cgaggcgagc aaatccgggc ggggcgtagg ggtctgggca acggcctggg gtacaaacga
     1381 agcaacgttg gtcgcgaaga tggcgagtga cagggtgccg gcggcggcgg tgagggcgag
     1441 aacggtcctc gccgaaactc cgtgtctcat ggttcaaggt ctccggaagg gtagataacg
     1501 gtctttcggg accgttaccc gagatatgtt ccgttactgc ggctaattca ccagacctct
     1561 tagcctagcg tccaggcccc ccggtcaggc tgcgagaaat actaaaattc ccttcatgtt
     1621 cacgtccagc acccggcgcg tcgactcgat tccccagcgg ccccggaccg cttcgccgaa
     1681 ccgcttggcg ctgaggtaac gcgtgcaaag gtagtaacgc acctcgtcct actcggtgcc
     1741 gtgcttggga tcccaatgac cctcgggtca cgagcacgcc gctcatctac actcgccacg
     1801 atcagatcgt ccgcctcttg gtggaatccg gtgccgacgt gaacgccagg gggaccagcg
     1861 gcgaagtgga attgcccgac tggtttttga cgccgctgtg gcgagcagcg cacgacggtc
     1921 ggctcgcgag tgtccgcctc ttggtcgagc gtggggcgaa cgtgaatcac aggaacccgg
     1981 acgggagtca ccaggcactc aagaccgctg cagaaaacga ccggcttgag acctgtgcgt
     2041 atctcatcga ccacggcgcc acgccggacc tcatcaccgc agcgatgctc ggcttggaca
     2101 cgcgggttcg tgaattgctc gaggaaagcc tggaagcgat gtctgcccgc gacgagcacg
     2161 gccgttccgc tctggatgcc gcaacccttc tggacagttt tcggcaatgt cgtgatggcc
     2221 ttcatcacgg gcatgatcgc gtggcgcacc tcttgattga gcacggatcg acagtcgagt
     2281 tggaacatgc cgccagcctc ggtctttgtg ccgagctcag gcgcatggtc gagttagacc
     2341 cggatattct caagcggccc aaggtcatga aagcactgat ggggggcaca gcaaccaagg
     2401 agtcgccgct gcaagcggca aggcgaaggg gccgcacgga aatcgtgtcc tatctgctcg
     2461 aacacggcgc agtcgatgtg ccagctgtca ttattgtcga gacttgacac tcgattttga
     2521 gcgtgcgg
//
LOCUS       DATAQL010000659         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2205564, whole genome shotgun sequence.
ACCESSION   DATAQL010000659 DATAQL010000000
VERSION     DATAQL010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205564"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VHS97_11520"
     CDS             complement(<1..650)
                     /locus_tag="VHS97_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448877.1"
                     /translation="MGIATRTNGTAPPDIALADIDLGTWEFWALDDDQRDGSFATLRR
                     EAPISFWGEPVQEGFAKGDGHWALTGLDDVFYASRHPEIFSSSPNITINDQTPEVSEY
                     FGSMIVLDDPRHQRLRSIVSRAFTPKVVARIETSVRERARRLVADLVAKHPDGTAEAV
                     SELAGPLPLQVICDMMGIPDEDHQKIFHWTNVILGFGDPDLTTEFDEFVQVSMEIGA"
     gene            760..1116
                     /locus_tag="VHS97_11525"
     CDS             760..1116
                     /locus_tag="VHS97_11525"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX3448878.1"
                     /translation="MARGSRPAQKLGRRNDPGVLIMTGLASGAKHGYALARDIERFAG
                     VTLGPGTLYGAITALEDNGLIEPVGHDERRRPYQLTAAGRAELTTAVRSLGAIAAEGA
                     RRLGIALHLPNPGCVQ"
     gene            1113..1490
                     /locus_tag="VHS97_11530"
     CDS             1113..1490
                     /locus_tag="VHS97_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448879.1"
                     /translation="MNPRDARRVLRWYPAAWRRRYGEELIAMLDDTHGDDELPRHARM
                     SLIRSGLIERVRLVRKYSGTYIGYSIACAFVWAVILTVVWTQVGDSTRHTFAVFFGGW
                     AIGWLSASIGRVVYPPPKSRRPV"
BASE COUNT          287 a          540 c          589 g          326 t
ORIGIN      
        1 gcaccgatct ccatcgaaac ctgaacaaac tcatcgaatt ccgttgtcag atcggggtcc
       61 ccgaatccga ggatcacgtt ggtccagtgg aatatctttt gatggtcttc gtcggggatg
      121 cccatcatgt cgcagatgac ctgcaacggc agcgggccgg ccagctcgct gaccgcctcg
      181 gctgttccgt cgggatgttt ggcgaccaaa tcggcgacca gccggcgggc tcgctcgcgg
      241 accgaagtct cgatgcgcgc caccactttt ggggtgaacg ctctgctgac gatggatcgc
      301 agccgttggt gtcgcgggtc gtcgagcacg atcatcgagc cgaagtattc ggagacctcc
      361 ggtgtctggt cattgatggt gatgttaggg ctggagctga agatctcggg gtgacggctg
      421 gcgtagaaca catcgtcgag cccggtgagt gcccaatgcc cgtcgccttt ggcgaagccc
      481 tcttgaacgg gctcccccca gaacgagatc ggcgcctcgc gccgaagcgt ggcgaacgac
      541 ccgtcgcgct gatcgtcgtc gagcgcccag aattcccatg tgccgagatc gatgtcggcg
      601 agtgcaatgt cgggcggcgc cgtgccgttc gtccgggtcg caatgcccat gtcggccgtc
      661 ctcgtcgttg agcgggctgt aggcgcgagc ctaaaccggt cgcggccgat cgggatacta
      721 cgcttggcgt agtacgttat acgtactaaa ctcctcggca tggcacgcgg ttcccgcccg
      781 gcccagaagc tgggcagacg aaatgacccg ggggtgctga tcatgaccgg cctcgcttcg
      841 ggcgccaagc acggctatgc gctggcccgc gatatcgagc ggttcgccgg cgtaacactg
      901 ggtccgggaa ccttgtacgg ggcgatcacg gcattggaag acaatggcct gatcgaaccg
      961 gtgggccacg acgagcgacg gcgcccctat cagttgaccg ccgcgggacg ggctgaactg
     1021 actacggcgg tacgcagtct cggggcgatc gcggccgagg gcgctcgtcg gctcgggatc
     1081 gcgttgcacc tgcccaatcc tggctgcgtg caatgaatcc ccgcgacgcc cggcgcgtcc
     1141 tgcgatggta cccggcggcc tggcgccgcc gttacggcga ggagttgatc gcgatgctcg
     1201 acgacaccca cggtgacgac gaacttccgc ggcacgcccg gatgtcactg atccgatccg
     1261 gcttgatcga acgagttcgc ttggtgcgca agtactctgg tacttacatc gggtacagca
     1321 tcgcgtgcgc gttcgtgtgg gcggtcattc tgacggtggt gtggacccag gtcggcgaca
     1381 gcacccgaca tacctttgcg gtgttcttcg gcgggtgggc catcggctgg ctatcggcga
     1441 gcatcggacg cgtcgtctac ccgccgccga aatcccgcag gccggtctga cgccggtctg
     1501 cgctgtgctc agtgtgagca gacggtgcgg gcaggcgccc cgttcgcggt cgcttggctg
     1561 accacgggct ttttcttgaa tcccaagttc ccatctgccg caattgaacc gatgcccaag
     1621 aatggtcgtg acaggcattg agagccgcgg gcgagcagca gggagctgat ctatggccga
     1681 gccatccgag ccatccggac gttggggcac ggcgcggccg ggccggcgcg acgtgctgcg
     1741 cg
//
LOCUS       DATAQL010000660         8399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_984312, whole genome shotgun sequence.
ACCESSION   DATAQL010000660 DATAQL010000000
VERSION     DATAQL010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984312"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1759)
                     /locus_tag="VHS97_11535"
     CDS             complement(50..1759)
                     /locus_tag="VHS97_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HEX3448880.1"
                     /translation="MSRTIALVERSNIAIHLSRMALRQPHTLAVVVPDGRGRGGRLRY
                     THMTYRQLDQDSDRLAHGFKAAGIGRGTRTVVMVRPSLDFFSLIFALFKAGIVPVLID
                     PGIGLSNLGRCCDEAAPEVFIGIPKALLARRLFGWGRRTVRHVIKVAPGSAVSFVPGV
                     KTLDQLRRMGAESRGQGLDPAPVIALLSPDEPAAILFTSGSTGAPKGAVYTHSIFQAQ
                     VEVFRTIYAIEPGEVDLCTFPLFALFAPALGMTAIVPDMDPTRPALVDPAKLFEAIDD
                     FGVTNLFGSPALLRRLSLGAERESRRLPSLKRVISAGAPVSAATLERVAHLLDHTAQV
                     YTPYGATEALPVASIGSRQVLSETRQATDSGLGVCVGRPVPGVQVRIIDLFDEPIPIW
                     SDALLVPDGVIGEIVVTGPVVTREYYNRSEATRLAKIIDPDGRGIHHRMGDVGYCDAQ
                     GRIWFCGRKSHRVVLEQETLFTIPCEAVFNTHPAVARTALVGVKRHGQIVPVICVELV
                     TRQSARARRQITHELLERGAAYPHTRWIQTVLFHASFPVDIRHNAKIFREKLAVWASR
                     RLS"
     gene            complement(1756..2667)
                     /locus_tag="VHS97_11540"
     CDS             complement(1756..2667)
                     /locus_tag="VHS97_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3448881.1"
                     /translation="MNSVLQRFLADHPGCDFDRGGLKLHYLDEGSGEPVVMLHGNPTW
                     SFYFRHLVDALRGSHRVIVPDHIGCGLSDKPDDSHYTYTLASRVDDLELLLDHLGLYR
                     DLTLVMHDWGGLIGMAYAARHPERIARLVVSNTAAFHKPSAKSMPRALAICRNSTVGA
                     FLVRGLNLFCNGTAWIGCKQQPMTRELRKAYIAPYDSWGHRIAVLRFVQDIPLSPADR
                     GYELVSWVQDRLGALRAVPMLLLWGMKDFVFDHHFLEEWTRRFPEARVHRFPHAGHYV
                     FEDEADAINGLVTSFLATQPAIEEHVG"
     gene            complement(2696..3748)
                     /locus_tag="VHS97_11545"
     CDS             complement(2696..3748)
                     /locus_tag="VHS97_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HEX3448882.1"
                     /translation="MKYANVYIESIGCEIAPVVVTSAELEDRLEGLYRALHLSSGQLE
                     ALTGIIERRWWEPGYRLSDGATAAGRRALAASHLGAEEIDVLIYGGVCREYFEPATAC
                     RVAALLGVGQEIAVYDVSNACLGVLTGMVEIANRIELGQIRSGMVVSCETAREINEIM
                     IDQMLKDKSMECFKRSLATLTGGSGAVAVLLTDGTLSTSKERRLVGGANRTAPHHHDL
                     CRWGIEPVELTGNRQFAQFTSTDATAVLTHGVELGKRTWQDFSRRLGWDHEHVNKVIC
                     HQVGAAHRETMLKSLSIPESKDFSTFAYLGNMGTVSLPLTAALAEDRSFLERGDRVAL
                     LGIGSGLNCMMLGVEW"
     gene            complement(3745..>8399)
                     /locus_tag="VHS97_11550"
     CDS             complement(3745..>8399)
                     /locus_tag="VHS97_11550"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX3448883.1"
                     /translation="VIVGAVDFAGDARAVITRCQLNSDPSRSNETLAAPTSGNPLVST
                     ACDGAVALVVKRLADAERDGDRIYAIIGDVATGCKGGNERTSDDRSRFSSVHEVENIE
                     RDLGHAGAALGLASVAKAALCLNRRILPNEGTGAGPSFWMRNRAEGPRRARVSVVSLG
                     GNLGHVMLDEYEDSPIPTQRDFRPALKQAGLFAIEADDEPGLVDRLRELREMAGALSA
                     RDIDTLARRWWQRHPNDPRLRLGKGIVADSVPSLERILDRVVQELHGQAGRAAAARAL
                     LVPKRLAFVYPGLGNHFAGMGRALAALFPEVLRTQDAESSFLREQLDPGVWWQDELPL
                     AFADHRVPIKGSVSVGALMTDVLRGLGVCPDAAIGYSLGESTALVALRAWTDRDELWR
                     RLQSSPLFHIELAGQCAAARRLWNIPATVPIDWVAGIVPRSAEAVRAAIGSRKQVYVL
                     IRNSADESVVGGQRSAVQEVIKKLQSPFVELPTVSTVHCEIARTVEADYRSLHDLDTT
                     APVGIDFYSGAWGRPYAVNRASAADAITAQSITTIDFPAVIERAYADGIGVFLEIGPG
                     SSCTRMISQILGGRPHLAFSACRPDGDPAVSLLEVLAGLISHRAAVDLAGLYDHSTEY
                     GNTLDCASSTGGDGPRRTIRVDVRGGAFQVPTLPSRQTDLQATIGYLMDEVLEETRPV
                     SNLDPIRSPLLRSVHDTEHATALAHQAFLRVAHGASELIGKQLSFQLKLIEELRHRAG
                     SDVWPTERMTKDNGALASTRSQTAPAWLDRPKCLEFAVGKIAGVLGPEFAEIDRFPTR
                     VRLPDEPLMLVDRILSIEGQRRSLQGGRVVTEHVIEPAAWYLDAGRIAPSIAIEAGQA
                     DLFLCGYLGVDFETKGLAVYRLLDATVTFHRSLPSAGEVIRYDIRITTFFRQGKTILF
                     RFQFDATVRGKPLLTMRDGCAGFFTPEELAAGKGIVPREIDRRPRRAPDLSDGVDLVA
                     MANERLDFHQLEALRRGDLAVAFGAPFDRVALLDPLPLPGGRMTLVHRVTTLEPAGGS
                     YGLGLIRAEADIHPDAWFMVCHFVDDRVMPGTLMYESCLHTLRIFLMRAGWIGSKDKV
                     AFEPVPGIANRLKCRGQIVESSRMVTYEVTIKERGYRPEPYAIADALILADGKPIVAV
                     SDMGLQLSGSSQAELEQLWKNGVTGEHTEASSDAKVTERPPASSTGPVLFDRERILAF
                     AIGKPSDAFGAAYRVFDEGRFVARLPGPPYQFLDRITRIDAEPLVMAAGGSAEAQFDV
                     APDAWYFQADRQDRVPFAVLLEAALQACGWMAAYMGSALKSDGDLKFRNLGGTARQHL
                     PVTRRTGTLTSKIRVAKISSTAGMILQHYEFAVYCRDGLVYDGSAEFGFFHPDALTQQ
                     VGIRDARPYELSTDEKREAESFPFPVGAPYPDSRWRMIDQVDALVLGGGRHGLGFVQG
                     STRVDPDAWFFKAHFLGDPVWPGSLGLESLLQLLKVVVVARFGGGSQALFESPALGQV
                     HRWTYRGQIVPTNKVVTVQAEIKAWDESKRLAVADGNLGVDGKTIYQMNDFSILTL"
BASE COUNT         1695 a         2694 c         2542 g         1468 t
ORIGIN      
        1 aggaagccgc cgccgccggt caccagaact tcaccaagct ccgcggcact catgacagcc
       61 tccttgatgc ccagacggcc agtttttcgc ggaaaatctt ggcgttatgg cgaatatcaa
      121 ccggaaagga cgcgtgaaac aagaccgttt gaatccagcg ggtatgtggg tacgccgcgc
      181 cgcgctcgag caactcgtgc gtaatctggc gccgcgctct tgcgctctgc cgcgtcacga
      241 gctcgacgca aatcaccggc acgatctggc cgtgccgctt gactccgacc aaggccgtgc
      301 gcgcgacagc ggggtgggtg ttaaagaccg cttcgcaagg aatggtgaag agggtctcct
      361 gttccagaac aactcggtgc gacttgcggc cacaaaacca gatccgacct tgtgcgtcgc
      421 aataaccgac gtcgcccatg cgatggtgga ttccgcgccc gtccgggtca attatcttgg
      481 ccagccttgt tgcctcggaa cggttatagt actcacgtgt cacgaccggg ccagtcacga
      541 cgatctcgcc aatcacgccg tcgggtacga gtagggcatc agaccagatc gggatcggct
      601 catcgaagag atcgatgatc ctgacttgca ctccgggaac tggccggccg acacacactc
      661 ccagccccga gtcggtcgcc tggcgggttt cactcagtac ctggcggctg ccgatcgacg
      721 cgactggcag tgcttcggtg gccccatacg gcgtatagac ctgggccgta tgatcgagca
      781 ggtgtgcgac tcgttcgagt gtggccgcgg aaacaggcgc acccgcggag atgacgcgtt
      841 tcaagctcgg caggcgccgg gattcgcgct cggccccgag cgaaagccgg cgcaacagcg
      901 ccggcgagcc aaacaagttt gtcacaccga aatcgtcgat agcctcgaag agcttggccg
      961 gatcgaccaa cgccggccgc gtcggatcca tatcgggcac gatcgccgtc attccgagcg
     1021 ccggggcaaa gagggcgaaa agagggaaag tgcaaagatc gacctcaccg ggctcgatcg
     1081 cgtaaatcgt gcgaaacact tcgacttgag cctggaagat ggaatgcgta tagacggcgc
     1141 ccttaggggc gccggtgctg ccgctggtaa agagaattgc cgccggctca tccggagaaa
     1201 gcagggcgat gacgggagcg ggatcgagcc cctgaccgcg agattcggcc cccatacgac
     1261 gcaactggtc gagcgtcttg acacccggga cgaagctgac ggcgcttccg ggcgcgacct
     1321 tgatcacatg gcgcacggtc ctgcgccccc agccgaacag ccgccgcgcc aagagcgcct
     1381 ttgggatgcc gataaagacc tccggtgccg cctcgtcaca gcaccggccc aggttagaaa
     1441 ggccaatccc cggatcaatt agcaccggca cgatgcccgc tttgaacaag gcaaaaatca
     1501 gtgagaaaaa gtcaaggcta ggccggacca tcaccacagt ccgggtgcca cggccgattc
     1561 cggccgcctt gaagccgtgt gcgagccggt cgctgtcctg gtcgagctgg cggtaagtca
     1621 tgtgcgtata gcgaaggcgc ccaccgcggc ctctgccgtc cggtacgacg acggccagcg
     1681 tgtgcggttg gcgcagcgcc atccggctta agtggatggc gatattcgat cgctcgacta
     1741 gggcgatggt tcgactcaac cgacgtgctc ctcgatggcg ggctgcgtcg cgagaaacga
     1801 ggtcaccagg ccgttgatcg cgtcggcttc gtcctcaaat acatagtggc cggcatgggg
     1861 aaaccggtgc accctcgcct cgggaaaacg ccgggtccac tcctccagaa aatggtgatc
     1921 gaacacaaaa tccttcattc cccacagaag cagcatcggt accgcacgca gggcgccgag
     1981 gcggtcctgc acccacgaga ccagctcgta gccgcgatcc gcgggcgaca agggaatgtc
     2041 ctgcacaaat cggagtacgg caatgcgatg gccccacgaa tcgtagggag cgatataggc
     2101 ctttctcagc tcacgcgtca tcggctgctg cttgcagccg atccatgcgg tgccgttgca
     2161 gaagagattc agccccctga ccagaaacgc ccctacagtc gaattgcggc agatcgccag
     2221 tgcccgcggc atcgacttcg cgctcggttt gtgaaacgcg gccgtattgc tcacgaccag
     2281 ccgagcaatc cgctccgggt gccgcgccgc gtacgccatg ccaatcaaac cgccccagtc
     2341 gtgcatgaca agcgtgaggt cacgatagag cccgagatgg tcgagtaaaa gctcgagatc
     2401 atcgacccgg ctggcaaggg tgtacgtata gtgcgaatcg tcgggcttgt cggacagtcc
     2461 acagccgatg tggtccggaa cgatgacgcg gtggctgcct cgcagcgcat cgaccagatg
     2521 ccggaagtaa aacgaccacg tcgggttgcc atggagcatg acgaccggtt caccggaccc
     2581 ttcatcgagg taatgaagct tgagcccacc acggtcgaag tcgcagccgg ggtgatcggc
     2641 caggaatctc tgcaaaaccg agttcatcgc cgcgctcgct cctctatatc gaggttcacc
     2701 attccacgcc gagcatcatg caattgagcc cgctaccgat cccgagcaac gccacccgat
     2761 ccccgcgctc caggaatgac cgatcttcgg ccaatgcggc ggtcagtggg agcgatacag
     2821 tacccatgtt gccaagatat gcgaacgtgg aaaagtcttt ggattccggt atagagagcg
     2881 acttgagcat cgtctcgcga tgggcggccc ccacctgatg gcagatcact ttattcacat
     2941 gttcgtgatc ccaaccgaga cgacgcgaga aatcctgcca ggtccgcttg cccagctcga
     3001 cgccgtgggt gagcacagca gtggcgtcgg tcgacgtgaa ctgcgcaaat tgtcgattgc
     3061 cagtcagttc cactggctcg ataccccacc ggcagagatc gtggtggtgg ggagccgtcc
     3121 ggttcgcccc acccacgagc cggcgctcct tcgatgtact cagcgtgccg tcggtgagca
     3181 atacggccac ggcgccggaa ccgccggtca aggtcgcgag cgaacgcttg aaacactcca
     3241 tacttttgtc tttcaacatc tgatcgatca tgatttcgtt gatctcgcgc gcggtctcgc
     3301 acgacacgac cattcccgag cgaatctggc ccagctcgat ccgattggcg atctcgacca
     3361 tgccggtgag cacacccaga cacgcattgc tgacatcgta aacggcgatt tcttgcccaa
     3421 cgcccaggag cgcggccacc cgacacgctg tcgccggctc gaaatattcc cggcaaacgc
     3481 ccccgtagat gaggacatcg atctcctcgg caccgagatg agatgccgct agcgcacggc
     3541 ggccggccgc ggtcgcaccg tccgacagcc ggtaaccggg ttcccaccac cgccgctcga
     3601 ttattccggt cagcgcctca agctgccccg acgaaaggtg cagcgcgcgg tagaggcctt
     3661 cgagccgatc ctccagctcc gccgaagtga ccacgactgg agcaatctca cagcctatgg
     3721 attcaatata gacgttggcg tatttcataa agtcaaaatc gaaaagtcgt tcatctgata
     3781 aattgtcttc ccatcaactc caagattccc gtcggccacg gccaggcgct tcgattcgtc
     3841 ccaagccttg atctcggctt gcacggtcac aaccttgttc gtggggacga tctggccacg
     3901 ataggtccag cgatgaactt ggcccaacgc cggcgactcg aagagcgctt gagagccgcc
     3961 gccaaagcgc gccaccacta cgactttcag caattgcagt aaggattcta accccagaga
     4021 accgggccaa accggatcgc ccaagaaatg ggccttgaaa aaccaggcgt cgggatcaac
     4081 gcgcgtgctg ccttggacga agcccagtcc gtgccggcct ccgcccaaga ccagggcgtc
     4141 gacttgatcg atcatccgcc agcgtgagtc agggtagggg gcgcccacgg gaaaggggaa
     4201 agattcagct tcgcgtttct catcggtaga cagctcgtac ggcctggcgt cgcggatccc
     4261 tacctgctgg gtgagtgcgt cgggatggaa aaagccaaat tccgcgctgc cgtcataaac
     4321 caagccatcc cggcaataga cagcgaattc atagtgctgg agaatcatgc ccgccgtgct
     4381 cgagatcttg gcaacgcgaa ttttggatgt gagcgtgcca gtccgccgcg tgaccggcaa
     4441 gtgctggcgc gcggtcccac cgaggttgcg aaacttgaga tcgccgtcgc tcttgagtgc
     4501 cgaccccatg taggcggcca tccagccgca cgcctgcagg gcggcctcga gcaagacagc
     4561 aaacggcacc cggtcctggc gatcggcctg gaaataccaa gcatcaggag ccacgtcaaa
     4621 ctgtgcctcg gccgagccgc cggccgccat cacgagtggt tccgcgtcga tccgcgtgat
     4681 ccggtcgagg aactggtagg gggggccggg caaccgcgcg acgaaccgcc cttcgtcaaa
     4741 gaccctatag gccgcgccga acgcgtccga cggcttgccg atcgcaaaag caaggatccg
     4801 ctcgcggtcg aacaacaccg gtccggtcga cgacgccggt ggccgctccg tgaccttagc
     4861 gtcactcgac gcctcagtgt gttcgcccgt cacgccattc ttccacaatt gctcgagctc
     4921 cgcctggctc gaccccgaga gctgaagccc catgtcggac accgcgacga ttggtttgcc
     4981 atcggccagg atcagggcgt cggcgatcgc gtagggctcg ggccgatagc cgcgttcttt
     5041 gattgtcacc tcgtaggtga ccatccgcga cgattccacg atctggcccc ggcacttgag
     5101 ccggttggcg atccccggca ctggttcaaa tgcaacttta tccttcgatc cgatccagcc
     5161 cgcacgcatc agaaagatcc gcagagtatg caggcaagac tcgtacatga gtgtacccgg
     5221 catgacccga tcgtcgacga aatggcaaac catgaaccac gcgtcgggat gaatatcggc
     5281 ttccgcgcgg atcaggccca agccgtatga acccccggcc ggctcgagcg ttgtcacgcg
     5341 gtgcaccaac gtcatccgcc cgcccggcag aggtagcgga tccaggagcg caacccgatc
     5401 gaacggggca ccaaaggcca ccgccagatc gccgcgccgc agcgcctcga gctggtgaaa
     5461 atcgagccgc tcgttcgcca ttgcgaccag gtcgacccca tcgctcaaat caggcgcccg
     5521 ccggggccgg cgatcgatct cgcggggtac gattcccttg cccgcggcca gctcttcggg
     5581 cgtgaagaag cccgcacaac catcgcgcat ggtcaagagc ggcttgcctc ttaccgtggc
     5641 gtcgaactgg aaccggaaca gaatcgtctt cccctgccgg aagaacgtgg tgatccggat
     5701 gtcgtagcga atcacctcgc cggcggaggg gagcgaccgg tgaaaggtca cggttgcgtc
     5761 caggagccga tagaccgcga ggcccttggt ctcgaaatcg acgcccagat aaccgcagag
     5821 aaacagatcg gcctggcccg cctcgatcgc gatactgggc gcaatccggc cagcatcgag
     5881 ataccatgct gccggttcga tcacgtgctc cgtgaccact cgaccacctt gaagggaccg
     5941 ccgttgcccc tcgatcgaca agatccggtc caccagcatc aagggctcgt ccggtagccg
     6001 caccctcgtc ggaaaccggt cgatttccgc gaattccggg ccaagcacgc ccgcgatctt
     6061 tcctaccgcg aactcgaggc acttcgggcg gtcgagccag gccggagccg tttgtgatcg
     6121 cgtcgaggcg agggctccgt tatctttagt catccgctcc gtgggccaaa catcgctacc
     6181 ggcgcgatgc cgcaattctt cgatcaactt cagctgaaac gacaactgct tgccgatcag
     6241 ttcgctggcg ccgtgcgcca cgcgcagaaa ggcttggtgg gctaatgcgg tggcatgttc
     6301 ggtgtcgtga accgagcgca agagtggcga acggatggga tcaaggttcg acaccggtcg
     6361 cgtttcctcg agcacttcgt ccatcaagta gccaatcgtg gcttgtaagt cggtttgacg
     6421 cgatggcagc gtggggacct gaaaagcccc cccgcgcacg tcgactcgga tcgtgcgccg
     6481 cgggccgtcg ccgccggttg aactggcgca atcgagcgtg ttgccgtact ccgtcgagtg
     6541 gtcataaagc ccggcgaggt cgaccgccgc tcgatgtgag atcaatccgg cgaggacttc
     6601 cagtagcgaa accgcggggt cgccgtcagg gcggcacgcc gagaaggcca ggtggggccg
     6661 cccgcccaga atttgactga tcatccgggt gcacgacgag ccgggaccga tttcgagaaa
     6721 cacgccgatg ccatcggcat aggctcgctc aatgaccgca gggaaatcga tggtcgtgat
     6781 cgactgagcc gtgatcgcgt ctgccgcgga agcgcggttg accgcgtagg ggcggcccca
     6841 ggcaccgctg tagaaatcaa tgcccactgg cgcggtcgtg tccaggtcgt gaagcgagcg
     6901 gtaatcggcc tcgacggtcc gagcgatctc gcagtgaacg gtactgacag tgggcagctc
     6961 aacgaacggg ctttgcagtt tcttgatcac ttcttgaacg gcgctgcgct ggcctcccac
     7021 gaccgattcg tcggccgagt ttctgatcaa cacataaact tgctttcggc ttcctatcgc
     7081 cgcccgtacg gcctctgccg atcgtggtac gatcccggcg acccagtcga tgggcaccgt
     7141 ggcaggaata ttccacagcc ttcgtgccgc agcgcattga ccggcgagct cgatgtggaa
     7201 gagtggcgac gactgaagcc ggcgccacag ctcgtcgcga tcggtccagg cccgcaaggc
     7261 tacgagcgcc gtcgattcgc ccaggctgta gccgatcgcg gcgtcggggc agacgcccaa
     7321 gcctcggagc acgtcggtca tcagggcgcc gaccgacacg cttcccttga tcgggacccg
     7381 atggtcggcg aatgcgagtg gcagctcgtc ttgccaccat acgccggggt cgagctgttc
     7441 acggagaaag ctgctttccg cgtcctgtgt ccgtaagacc tcaggaaaaa gggccgccag
     7501 agcacgaccc attccagcga aatggttacc cagcccgggg tacacaaacg ctagccgctt
     7561 cggaacgagg agggcacggg cagcagccgc gcgtccagcc tgtccgtgca actcctgtac
     7621 gacgcgatcg aggattcgct ctagcgatgg gacactgtcg gccacgatcc ctttgcccag
     7681 gcggagccga ggatcattgg gatgacgttg ccaccatcgt cgggccaatg tgtcgatatc
     7741 gcgagccgat agagccccgg ccatttcccg cagctcgcgc aatcgatcga cgagtccagg
     7801 ttcatcgtcc gcctcgatgg cgaacaatcc cgcttgcttt aatgccgggc gaaagtcgcg
     7861 ttgagtgggg attggcgaat cctcatattc gtcgagcatg acgtgaccta ggtttccccc
     7921 caggcttaca acactcaccc gagctcgccg tggtccttcc gcccgattgc gcatccaaaa
     7981 gcttggtccg gcgcccgtgc cttcgttggg caggatccgc cggttcaggc acagggccgc
     8041 cttggcgacc gatgccagac ccagggccgc ccctgcgtgc cccaggtcgc gctcaatgtt
     8101 ttcgacctca tggacagatg agaatcgcga tcgatcgtca cttgttcgtt cgtttccgcc
     8161 tttgcaaccg gtagccacgt cgccgatgat cgcgtagatc cgatcgccgt cgcgctctgc
     8221 atccgccagg cgtttgacga ccagggccac tgccccgtcg caggcggtcg aaacgagggg
     8281 attgccggac gtaggggccg cgagcgtttc gtttgaccga ctgggatctg aattaagctg
     8341 gcaccgagta atgaccgcgc gggcgtcgcc ggcgaagtcg accgctccca cgatcacgg
//
LOCUS       DATAQL010000661         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2509646, whole genome shotgun sequence.
ACCESSION   DATAQL010000661 DATAQL010000000
VERSION     DATAQL010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2509646"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1550
                     /locus_tag="VHS97_11555"
     CDS             99..1550
                     /locus_tag="VHS97_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3448884.1"
                     /translation="MARRTGCSFAMIGSIGLLCWQASSDAKPKDEMLELYGMFVDAVE
                     KVETNYVRPVSRRELLESALDGMLQNLDAHSSFINTGEWRQFRRQIEGKFGGIGIQVG
                     LDEESGRLRVIAPMVGTPAYEAGILAGDQIVEIEGQSAEGMSPDKAAEFLMGRPGTDV
                     KLSVLHEGTEDPEPMTITRAIIEVPTVLSDRRKPDDQFDFMLDKDKKIGYVRIGTFSQ
                     NTAEELKKALEQLKEAGVRGLVLDLRDDPGGLLSAAVEVADMFLEKGEIVSTKGRNTA
                     PKKYDAQKDGLYEDLPLVVLVNQNSASASEIVSAALQDHKRAVIVGQRSYGKGSVQNI
                     IDLDGGNSVLKLTVASYYRPSGENIHKFKNAKNSDKWGVSPDKEAEVKLPHSEYVRWF
                     IARRERDMESAAKGHRKATNNTPPTEKAKPAEGEKPSEKGEAKADDKKDEDKTKAKAK
                     SPHEPAGPFVDKQRDKALEIIRAKIAEAKPKAA"
     gene            1577..2653
                     /gene="tsaD"
                     /locus_tag="VHS97_11560"
     CDS             1577..2653
                     /gene="tsaD"
                     /locus_tag="VHS97_11560"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX3448885.1"
                     /translation="MSIDQDKLILLAIETTCDETAAAVLEGPRPPGIGVPRIRSSVVS
                     SQVGLHQRYGGVVPEIASRAHVRQVVPMIDQALDRAGVSLARLDAVAVATRPGLVGAL
                     VVGLTAAKALALALDIPLVAIDHLEGHLYACQLAYPDRDVYPCVGLVVSGGHTSLYVC
                     DSPLASEYLGGTTDDAAGEAFDKVASLLGLGYPGGPVIEQAARAGNPAAFAFPRSFLH
                     DDRLIWSFSGLKTAVLYALGWQNARHAPNELGQKLKSDVAASFQEAVIDVIVAKTRQA
                     LMRTTIKKLGVGGGVAANAALRQRLTEMAAALGVEVFIPPPTLCTDNAAMGGIALPKL
                     AAGQVASLDIDVAAGLVRTKQRQH"
BASE COUNT          600 a          788 c          863 g          474 t
ORIGIN      
        1 cacggctgcc gctcggtcgg aacaaccgac caaagacctg ccgcagatcg atcgcttcga
       61 ttcggtcgaa cgatgatgaa taaggagggc actcgctgat ggctcgccgg actggttgtt
      121 cattcgcaat gatcggatcg atcggcctgt tgtgctggca ggccagctcc gatgccaaac
      181 ccaaggacga gatgctcgag ctgtacggca tgttcgtcga cgcggtcgag aaggttgaaa
      241 ccaactacgt ccgcccggtg tcgcgccgcg agttgctcga gagtgccttg gacggaatgc
      301 tccaaaatct ggatgcgcac tcgtcgttta tcaataccgg cgagtggaga cagttccggc
      361 gccagatcga gggaaaattt ggcggaattg gcatccaggt ggggttagac gaagagagcg
      421 gacggctccg cgtgattgca cccatggtcg gaactcccgc gtacgaggcg ggtatcttgg
      481 ccggcgacca gatcgtggag attgaaggac agtcggccga aggcatgagc ccggacaaag
      541 cggccgaatt tctgatgggg cgcccgggca ccgacgtcaa gctcagcgtc ttgcacgagg
      601 gcacggaaga tcccgaaccg atgaccatca cacgcgcgat catcgaggtt cccaccgtac
      661 tctccgaccg ccgcaagccg gacgatcagt ttgatttcat gctcgataaa gacaagaaga
      721 tcggctacgt ccggatcggg accttcagtc aaaacaccgc cgaggaattg aaaaaagccc
      781 tcgaacaact caaggaagca ggggtccgcg ggctggtgct cgacctcaga gatgatccgg
      841 gcggtttgtt gagcgcggcg gtcgaggttg ccgacatgtt cctcgaaaag ggcgagatcg
      901 tcagcaccaa aggaagaaac acggccccca agaagtacga cgcccaaaag gacggcttgt
      961 atgaagacct gccactggtc gtgttggtga atcaaaactc ggcgtcggcc tccgagatcg
     1021 tttcggccgc gcttcaagat cacaagcggg ccgtgatcgt cggccagcga tcgtacggta
     1081 aggggtcggt tcaaaacatc atcgatctcg acggcggcaa cagcgtgctc aagttgacgg
     1141 tggcaagtta ctaccgtcca tcgggcgaga atatccacaa gttcaagaac gccaagaaca
     1201 gcgacaaatg gggtgtttcg cccgataaag aggcggaggt caagctcccc cacagcgaat
     1261 acgtccgctg gttcatcgcc cggcgcgaac gtgacatgga atccgccgcc aaggggcacc
     1321 gcaaggcgac gaacaacact cctccgaccg agaaagccaa accggccgag ggagaaaagc
     1381 cctcggaaaa aggcgaagcc aaagccgacg ataagaagga cgaagacaag acgaaggcga
     1441 aggcaaagtc accgcacgaa cccgccggtc cgtttgtcga taagcagcgc gacaaggctc
     1501 tcgagatcat ccgagccaag atcgccgagg cgaagcccaa ggcggcttga gtcgctcaag
     1561 cccccggtcg gcggtaatgt ccatcgatca agacaagctg atccttctgg ccatcgagac
     1621 gacgtgcgac gagacggccg cggcggtcct ggagggtccg cggccgccgg gaatcggtgt
     1681 gccccggata cgttccagcg tggtgtcatc gcaggtcggg ctgcatcagc gctatggagg
     1741 cgtggtaccg gagattgcct cgcgggcgca cgtccggcag gtcgtgccga tgatcgacca
     1801 ggcgctcgac cgggccggcg tctctctcgc caggcttgat gcggtggccg ttgcaacgcg
     1861 gcccgggctg gtcggtgcct tggtggtcgg gctgacagcg gccaaggcac tggcgctggc
     1921 gctcgatatc ccgctggtgg cgatcgatca tctcgaaggt catttatacg cctgccagtt
     1981 ggcgtaccct gatcgtgacg tttatccgtg cgtggggctg gtcgtatcgg gaggacacac
     2041 gagcctctac gtctgcgaca gcccgcttgc gagcgagtat ctcggcggta cgaccgacga
     2101 cgccgcaggc gaagcctttg ataaagtggc gagcctgctg gggttgggat atcccggagg
     2161 tcctgtgatc gaacaagcgg cccgggcggg caatccggcg gccttcgctt ttccccgatc
     2221 ttttttgcat gacgaccggc tcatctggag tttctcaggt ctgaaaacgg cggtccttta
     2281 tgcattaggc tggcaaaacg cccgccacgc tcccaacgag ttgggacaga agttgaagag
     2341 cgacgtcgcg gctagctttc aagaggccgt catcgacgta atcgtggcca agactcgtca
     2401 ggccttgatg cgcactacaa taaagaagtt aggcgtggga ggtggcgtgg cggcgaacgc
     2461 cgcgctccgg caacggctca ccgagatggc cgccgcgctg ggcgttgaag tcttcattcc
     2521 gccgccgacg ctctgtaccg acaacgccgc catggggggg atcgcgcttc ccaagctcgc
     2581 cgcggggcag gttgccagcc tcgacatcga cgttgcggcc ggcttggtac ggacaaaaca
     2641 gcgtcaacat tgaactgatg aatcggctca actgaaccct caccgacttt cctgatcaaa
     2701 aaggccagtg taaactacct atcct
//
LOCUS       DATAQL010000662         5156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4037430, whole genome shotgun sequence.
ACCESSION   DATAQL010000662 DATAQL010000000
VERSION     DATAQL010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4037430"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VHS97_11565"
     CDS             complement(<1..196)
                     /locus_tag="VHS97_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX3448886.1"
                     /translation="MRVIRADVMGMCFGVRDALELIDGIAEPRAVTIHGQLVHNEIVQ
                     SRLESKGFALRDEPERAQSVP"
     gene            complement(342..746)
                     /locus_tag="VHS97_11570"
     CDS             complement(342..746)
                     /locus_tag="VHS97_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX3448887.1"
                     /translation="MPHPKARSTQSNGRFAYEGLERVFHEKARLGIMTSLVSQPRGVV
                     FADLKEMCHLTDGNLSRHLQVLNEAGLVELWKGQHNNRPQTLCRITDEGRRRFFEYIN
                     VLETVVHDAMNAARDNSAPAPKVSFADGWSPA"
     gene            complement(733..1347)
                     /locus_tag="VHS97_11575"
     CDS             complement(733..1347)
                     /locus_tag="VHS97_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448888.1"
                     /translation="MELRDALTQITEIRLQLARTEVFRGYRAMPVAFSGGVAVLAAVI
                     QTATIADPAVQIGPYLGLWIGAAVVSALAAGLEMMIRARNSASTMTRELTWLALEQFC
                     PCLIGGALVTIVLLRASPESLWMLPGLWQIFYSLGIFASCRLLPRPTFWVAVFYLGTG
                     LLVLALPHGEAALSPWAMGVPFGAGQMLAAAVLYCTLERDHASS"
     gene            1677..3050
                     /locus_tag="VHS97_11580"
     CDS             1677..3050
                     /locus_tag="VHS97_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX3448889.1"
                     /translation="MGYTEVLLIAGLILINGFFVTVEFALVKVRTTQIDQLAEEGNWA
                     AKLTSKALDRLDFYLSASQIGITVASLALGRAMHDWVEPLFESAFRQNKILGSPIAIF
                     GLSISTATLAALSLVTFLHMALGEQAPKNLAIRNPKVLVLLTAPPLVILSYVFSPVIW
                     LLNKASNLTLWALGLGQVSTSELAHSEEELRLIVAESVAGGHLSRNERMMIENVLNLE
                     EKTARRVMVPRPDIVYLSLARPLEDNLRLARQAGHTRFPLCEDDLSTVIGMIHVKDIF
                     RAGAYQNGRTEMRQLARKVPFLPVTLRLDQLLLEFQRNRVHLAMLLDEYGSVVGMATL
                     ENVLEELVGPIQDEFDRETPPITPLGNNVYEVDAACPLDVLAETTGLEIPDTDAETVG
                     GLVLDLLGRLAKPGDSVTINGYRVTVVRADPTRIRKIRLEPIGAEAASSPTSDATHSA
                     ANPENHG"
     gene            complement(3314..4870)
                     /locus_tag="VHS97_11585"
     CDS             complement(3314..4870)
                     /locus_tag="VHS97_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3448890.1"
                     /translation="MTNLTPEERVLGRENANRALGVSRRDVLKAAAAAPALGAFYFGY
                     DRMNSKDPVRAAIIGTGDEGCQAMIRSHNREYLDFVGYCDIRPSQQERAVKEFSNHAE
                     YSSSDVKKLKKYATKDEMYADPDVEVIVIALPLWLHAPVAIEAMNKGKHVFTEKLMGH
                     SVAECKEMCQVARKTNRLLAVGHQRHYSVLYDNANYLVKNEVLGDIRHIRALWHRNNA
                     QPLLAKDKNGNPIYDPKTGLAEIVHDEEGKPVYRDSWKRIHTDLDKNIDYKKYGYASL
                     DELINWRLYNRTGAGLMAELGSHQLDACSIFLGKRHPLAVTGIGGTFFYTDGREVDDH
                     VFTVFEFPGTGERDRVVVTYSSINTNSFDGYGEQVMGSKGTMIVSQEKEILLYKEAGS
                     PALSRSTSVSVESQGKKPALETSPSTAGPTAAAALGGLATAEPSRGYREELEHFAFCV
                     RHGNSSDYHADKDHQPRCRGEVALADAVIALTSNIAMRQKRRIDFDPRWFDYQSPETP
                     ESAPSIAQRS"
     gene            complement(4930..>5156)
                     /locus_tag="VHS97_11590"
     CDS             complement(4930..>5156)
                     /locus_tag="VHS97_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448891.1"
                     /translation="WPGTPPNPRAEGHYMIVSKNLVELLACLVIATTPSGHWVGFDAL
                     FFGARRRRRLARNLARAETNAARRQRIRVI"
BASE COUNT         1031 a         1561 c         1488 g         1076 t
ORIGIN      
        1 cgggcacgga ctgcgcgcgc tctggctcgt cccgcagcgc aaatcccttc gattccagcc
       61 gcgactgcac gatctcgttg tggacgagtt gtccgtggat cgtgacggcg cgcggttcgg
      121 cgatcccgtc aatcagctcg agggcatcac gcacaccgaa acacatcccc atcacgtccg
      181 ctcgaatcac acgcatcatg cctccttccg cggcgatgac tccgggcagc cctcgattga
      241 accgcggcgg caagtccgtc gttttcgcca attgctttgt gacgccaagg aaaatttcac
      301 aaatgcctta ccggcgggtg agcgtgccgg cgatcgatga ttcaagcggg cgaccaaccg
      361 tcggcgaaac tcaccttggg ggcgggcgcc gaattgtcac gcgcggcgtt catggcatcg
      421 tggacgacgg tctcaagtac gttgatatat tcaaagaaac ggcgacggcc ttcgtcggtg
      481 atccggcaaa gcgtttgggg acggttgttg tgttgtccct tccagagttc gaccaggccg
      541 gcctcattaa gcacctggag gtggcggctc aagttgccat cggtgaggtg gcacatctcc
      601 ttgagatcgg cgaagacaac cccgcgcggc tgcgagacca gcgaggtcat gatgccgagc
      661 cgggcctttt cgtggaagac gcgctcgagc ccctcgtacg cgaaccggcc gttggattgg
      721 gtcgatcggg ctttaggatg aggcatgatc gcgctccaat gtgcaataaa ggaccgcggc
      781 ggcaagcatc tggcccgctc caaacggcac gcccatcgcc cagggagaaa gcgccgcctc
      841 gccatgcggc agggccagca caagaagtcc ggtacccaga taaaacacgg ccacccagaa
      901 cgtcggcctg ggaagcaagc ggcaggaagc aaagatccct agactataaa agatctgcca
      961 aagtccgggc agcatccaga gactttccgg tgaggcacgt aacagcacga tcgtcaccag
     1021 agctccgccg atcaagcacg ggcagaattg ctctagcgcc agccaggtta gctcgcgggt
     1081 catggtcgag gccgagttgc gcgcccggat catcatctcc aggcccgccg ccaaggcact
     1141 cacgacagcc gctccgatcc atagccccag ataagggccg atttgcacgg ccgggtccgc
     1201 gatcgtcgcc gtctgaatca ccgccgccag caccgccaca ccgcccgaaa acgccacagg
     1261 catcgcccga tagcccctga agacctcagt cctggcgagt tgcagccgaa tttcggtaat
     1321 ctgggtcaac gcatcacgca gttccatggg tgttaaccct tacgcaccca atgcccgggg
     1381 atcaaaatga cgccaatttg gtagcttctc acggatcgat caccaccgtc actttacacc
     1441 ataaagtttg tttggcgagg tgtcaagatg ccccggcaaa tcgagcttgt cgttcgagtg
     1501 attcgcgcta gacgcgattt ctggagtgcg actccggtag gttttggtta gaatcggcgt
     1561 gcttgctggt ctccaattgt cactgattca tcgttgagga cctaccggcc gcgtgtgtcg
     1621 acttctccct tcaaccttgg cctttcaagc ctcgtaaaga ccggattgct cccgaaatgg
     1681 gatacactga ggtcctgctg atcgcgggtc tgatcttaat caacggcttc ttcgtcactg
     1741 tcgaattcgc cctggtcaag gtccggacaa cccagattga ccaacttgcg gaagagggaa
     1801 actgggcagc caagctgacg agcaaagcgc ttgatcgtct cgatttctat ctttcggcat
     1861 cgcagatcgg gattaccgtc gccagcctgg cgctggggcg cgccatgcac gattgggttg
     1921 aacctctatt cgaatcggcc ttccgccaga acaagattct cgggagcccg attgcgatct
     1981 tcggtctctc aatatcgacg gcaactttgg cggccctgtc gctggtcacc ttcttgcata
     2041 tggcgctggg agagcaagca cccaagaacc tggcgattcg taatcccaag gtcttggtgc
     2101 tattgactgc tccgcctctg gtcatccttt cgtacgtctt ctcgccggtg atctggctct
     2161 tgaacaaggc aagtaacctg actctctggg cgctgggcct cggtcaggtc agcacttcgg
     2221 agctggccca cagcgaagaa gagctgcggc ttatcgtggc cgagagcgtg gccggcgggc
     2281 acctctcgcg caacgaacgg atgatgatcg aaaacgtgct caacctggag gagaagaccg
     2341 ccaggcgcgt catggtaccc cggcccgaca tcgtctattt gagcctggct cgaccactgg
     2401 aagataacct gcgcctggca cggcaggcgg gacacacccg ctttccactc tgcgaggacg
     2461 acctgtctac cgtgatcggc atgatccacg tcaaggatat cttccgggcc ggggcttatc
     2521 agaatggccg aacagagatg cgccagctcg cacgtaaggt cccgttccta ccagtcaccc
     2581 tccggcttga ccagctcttg ctcgagtttc agcgcaatcg agttcatctc gccatgctgc
     2641 tggatgagta cggaagcgtg gtgggcatgg cgacgctgga aaacgtgctt gaagagctcg
     2701 ttggtccgat ccaggatgaa ttcgatcgcg agacacctcc gatcacgccg ctgggcaaca
     2761 acgtctacga ggtggacgct gcctgcccgc tcgatgtgct cgccgaaacg accggcctgg
     2821 agattcccga caccgatgcc gagactgtcg gcggcttggt cctcgacctg ctcggacgcc
     2881 ttgccaagcc cggcgacagc gtcacaatca acggctatcg ggtgaccgtc gttcgggccg
     2941 atcccacgcg gatcaggaaa attcgactgg agccgatcgg cgccgaggcg gcgtcgagcc
     3001 cgacctcaga tgcaacgcac tcagccgcca accccgaaaa ccacggctga gcccaattcg
     3061 cgaagtaact cccccgttct tatgtgagtc aaacggcaac gatcacgcca tcgacacgtc
     3121 gggacccatt cgcagagaaa acgcgtttag cgaccggaaa acgcgcgcac atacttggag
     3181 ttgacaaaaa tcggttctac gcctcatttc cttacgcagt cacaagttgc gcatgtcgct
     3241 ggaatatgag gcgcataatc gtcagcctgg aaatgctgac ctacgtcgcg ccgcggcgtt
     3301 gagcagtgtc tgtttaactg cgctgagcaa tgctcggggc gctttcaggt gtctctggtg
     3361 actgatagtc aaaccagcgc gggtcgaaat cgatccggcg cttctgtcgc atggcgatgt
     3421 tgctcgtgag tgcgatgacg gcgtcagcca gggctacctc gccgcgacag cgtggttggt
     3481 ggtctttgtc ggcgtgatag tccgacgagt tgccgtggcg cacgcagaat gcaaaatgct
     3541 caagctcttc ccggtagccg cgcgacggct ctgctgtcgc caagcctccc agcgcggccg
     3601 cggcggtcgg accggccgtg ctcgggctgg tttcaagcgc gggcttcttg ccctgcgatt
     3661 cgaccgagac gctggtcgat cgcgacaagg ccgggctgcc ggcttctttg tacagcaaga
     3721 tctctttttc ctggctcacg atcatggtcc ccttcgaacc catgacctgc tcgccatagc
     3781 cgtcgaacga attggtgttg atcgacgaat aggtcacgac aactcggtct ctttctccgg
     3841 ttcccgggaa ctcgaaaact gtgaagacat ggtcgtccac ctcgcggcca tcggtgtaga
     3901 agaaagtacc gccgatacca gtcacggcca gcggatgccg cttgcccagg aagatcgaac
     3961 aggcgtcaag ctggtggctg cccaattccg ccatcaagcc cgcgccggtg cgattgtata
     4021 aacgccagtt gataagttcg tcaaggctgg cgtaaccata tttcttgtaa tcgatattct
     4081 tatccaagtc ggtatgaatg cgcttccagc tatcacggta gacgggcttc ccttcctcgt
     4141 cgtggactat ttcggcaagc cccgttttgg ggtcgtagat cgggttgccg ttcttgtcct
     4201 tggcgaggag cggctgagcg ttgttgcgat gccaaagggc tcgaatgtga cgaatgtcgc
     4261 ccagcacttc gttttttacc agataattgg cgttatcgta gagtacggaa taatgtcgct
     4321 ggtggccgac ggccagcaat cgattggtct tgcgggcgac ctggcacatc tccttgcatt
     4381 cggcgaccga gtggcccatc agcttttcgg tgaagacgtg cttgcctttg ttcatcgcct
     4441 cgatcgccac aggcgcgtgc agccagagcg gcagcgcgat cacgatcacc tcgacgtcgg
     4501 gatcggcata catttcgtcc ttggtggcat attttttcag cttcttgaca tcgctcgagc
     4561 tgtattccgc atggtttgaa aattccttga ctgcccgctc ttgctgcgag ggacgaatgt
     4621 cgcagtaccc cacgaaatcg agatactctc gattgtgcga gcggatcatc gcctggcagc
     4681 cttcgtctcc cgtgccaatg attgccgccc gaaccggatc cttgctgttc atacggtcgt
     4741 agccgaagta aaacgctccc agggccgggg cggcggcggc cgccttcaag acgtcacgtc
     4801 gcgaaactcc cagcgcccga ttggcgtttt cgcgtccgag cacgcgctct tccggtgtca
     4861 ggttggtcat cgatcgctcc ttttgaagat ggaaaccaaa gtttatttca gagtatgttc
     4921 gagtgcaatc taaatcacac gaattcgttg tcgcctggcc gcattagttt ccgcgcgggc
     4981 gaggttgcgc gccagccgtc gccgccgccg cgcgccgaaa aagagcgcat caaagcccac
     5041 ccaatgaccg ctgggtgtcg tggcgatcac cagacacgca agcagctcga ccaggttttt
     5101 gctgacgatc atgtaatggc cctcggccct cgggttagga ggcgtgccag gccatg
//
LOCUS       DATAQL010000663         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2816422, whole genome shotgun sequence.
ACCESSION   DATAQL010000663 DATAQL010000000
VERSION     DATAQL010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816422"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..800)
                     /locus_tag="VHS97_11595"
     CDS             complement(60..800)
                     /locus_tag="VHS97_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEX3448892.1"
                     /translation="MAPAPAPADVGIVAALPIEVSDLVDGLKKVWKYKSVAVPVIEGE
                     YAGKIVAVSIAGMGRAAARRATDVLVAGHRPRWVISAGFAGALNPAYARNDLVLAREV
                     IDRDGGCYAVELPPGLAANVKYSTGRLLTVDQLVLRAAEKDELYRTYQADLVDMESSA
                     VAAICREQLVRFLAIRVISDDAHGDLPAEVSGIINSSGSYRVGAALRSVWRRPSSIKD
                     FWSLYEQSLEAADRLSKFIKRCLDELPV"
     gene            1247..1909
                     /locus_tag="VHS97_11600"
     CDS             1247..1909
                     /locus_tag="VHS97_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3448893.1"
                     /translation="MSQATIDPPDIKTLADLRRRLGGIPLERIWFRPAPGTATEKDVI
                     LAEERENRLCELVDGTLVEKAMGFEESRLAVELAYLVKSYLDQHDLGICVGADGMMRI
                     APGLVRIPDLSFITWDRLPGRESPQEPIPDLAPDLAVEVLSMGNTKPEMARKVREYFE
                     AGVVLVWLIDPKKRTAQVFSTGEKPVLIRADQALDGGDVLPGFVVLLSDLLDHGRRPR
                     SG"
BASE COUNT          354 a          601 c          633 g          370 t
ORIGIN      
        1 tgccatggtg cgcgcttgat acccccgtct ggagtacgag ccggtgacga gtttgcggtt
       61 tagaccggca gttcatcgag acatcgcttg atgaatttcg acaagcgatc ggccgcctcg
      121 agcgattgct cgtaaagaga ccagaaatct ttgatacttg aaggtcggcg ccaaacgctg
      181 cgcagggccg ctccgacgcg atagctgccc gaagagttga taatccccga cacttcggcg
      241 ggtagatccc cgtgagcgtc gtcggagatc acgcggatcg ccaggaaacg cacgagttgc
      301 tcgcgacaaa tggcggccac tgcggacgat tccatgtcga ccagatcggc ctggtaggtc
      361 cggtagagct cgtctttttc ggcagcgcgg agcacgagct ggtcgacggt gagcaggcga
      421 cctgtggaat atttgacgtt cgccgccagt ccggggggta gttcgacggc gtagcaaccc
      481 ccgtcccggt cgatgacttc gcgggcgagg accaagtcgt tgcgggcgta ggcggggtta
      541 agagcaccgg cgaaacccgc cgaaatgacc cagcgggggc ggtggccggc gacgaggaca
      601 tcggtcgcgc ggcgggctgc cgcgcgtccc attccggcga tggacacagc gactatcttg
      661 cccgcgtatt cgccttcgat caccggcacc gcgacggact tgtacttcca caccttcttg
      721 agcccgtcca ccaggtcgct cacctcgatc ggcagggccg cgacgatccc cacgtcggcg
      781 ggagccgggg ccggcgccac gccacgtccg tccgggttgc cggaacgact ctcacctgtc
      841 gagcgatctt ccatcgtgag agcggcggct cacttcatat cagaagaatg gggattggac
      901 tacttgatac gttacacagc aaactggtca cgctgacgac gcacgacctc gcgttgccag
      961 ggcgcacctc gacactttag atcctgacat tagttccgta acaataattg gccagtggcc
     1021 gtggtacaag accccgttga gcgacagcgc gacgcctggg gtggtctgat tgaagtttcg
     1081 ttcggtcctc gatcggtcgg tcctccggcg tgcgagccag gcacccgaca gcagtaacgc
     1141 cttgccgggc gttggtcctc ggccgccgtt ccttgccgat caaactgcga ggtgtcagaa
     1201 ttgtggggag acgcttcgag gtatcgcgaa ccacgaggaa gccgtcatgt ctcaggcgac
     1261 aatcgatcct cccgacatca agacgctggc tgacctgcgg cggcggctgg gtgggatccc
     1321 gttagagcgg atctggtttc gccccgcgcc cggcactgcc accgagaagg atgtcattct
     1381 tgccgaggaa cgagagaacc gtctctgcga gctggtcgac ggcactctgg tggagaaggc
     1441 catggggttc gaggagtcgc gactggctgt cgaactcgcg tacttggtca agtcttatct
     1501 cgaccagcat gacctcggaa tctgcgtcgg ggccgacggc atgatgcgga tcgcgcccgg
     1561 cctggtgcgg attcctgacc tgtcgttcat cacctgggac aggctccccg gccgtgagag
     1621 cccccaagag ccgatccctg acctggcgcc cgacctggcc gtcgaagtgc tcagcatggg
     1681 gaacaccaag ccggagatgg cccgcaaggt gcgggagtat ttcgaggctg gcgtcgtgtt
     1741 ggtctggctg atcgatccca agaaacggac cgcccaggtc ttctccactg gcgaaaagcc
     1801 agttctcatc cgcgcggatc aggcgctcga cggcggtgac gtcttgccgg gcttcgtcgt
     1861 tcttctctcg gacctgctcg accacggccg gcggccacgg tcgggttgat cgccgggtct
     1921 ccctctcaca attcgagcga caagggttgt gccgggat
//
LOCUS       DATAQL010000664         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3276034, whole genome shotgun sequence.
ACCESSION   DATAQL010000664 DATAQL010000000
VERSION     DATAQL010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3276034"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VHS97_11605"
     CDS             complement(<1..198)
                     /locus_tag="VHS97_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448894.1"
                     /translation="MNKVSRDNTSKQPGLQATPSSSGWLGSIFLGLALFLVYMSNGRE
                     LGTYDTISASLLPLRILRGDGI"
     gene            complement(319..>1858)
                     /locus_tag="VHS97_11610"
     CDS             complement(319..>1858)
                     /locus_tag="VHS97_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448895.1"
                     /translation="LEQRECKPRTRRSAAAQGHSAVSLNQGSLVAIHPLAMENDQMAA
                     VAASFAGIDREWNPSAPMTENTMIRRRGMRDVNAARLRHVTAGAVIVAAALQPVARWK
                     AATPLSVTLETPIPVIRNLLRGGGKMVWIVARNAPEPTATGAKAIALLHLFKLADKTV
                     FGLVRRLNEHRPEAKKRQPWSIVFISPTNAHNPRITGQVALRTDVIPERWLQIGRIDD
                     RHVLTVDHRGTCDVKLAWPMTAFTADCVTLEDRLSILVDRAFPRLDPIRVAEQAIALD
                     RPVEVIVDGFKTRRQAPLLLPGIPGKRGLEKKAIVLDQVGRSLPARANREPDLGLALG
                     DNPARCIASPLLVKDVTVPMFDGVLEALTLEQGLGVSLVALLYVVLADGCEGAAHRVL
                     AEGKSTLRVAANTRRIPEILYLRAGVLVGRDRNFPTDAFRLLGSPRNEDHPRAYHNER
                     SEKHKPLRSQSRRSVLGIDRALQLAVLVSARSGLTPAQRTLRRTNRMFVSDSTMTLAG
                     PANI"
BASE COUNT          372 a          543 c          580 g          363 t
ORIGIN      
        1 aatcccatcg ccacgcagaa tgcggagggg cagcaaactc gccgaaatcg tgtcgtaagt
       61 tcccaactcc cggccattgc tcatgtagac gaggaacaga gccaggccca agaagatcga
      121 gcccagccag ccagaactag agggagtcgc ctgcaatccc ggctgcttcg aagtattgtc
      181 acgcgatact ttgttcatcg atgctagttg atgcccatcc atggcggagt cggccacgac
      241 cgacgaagtt ggacgaacgc tcgggggcca aaattgagcg ttcaagggcg atcagggctc
      301 aatccggcct ctaaactatc atatgttcgc gggcccggcg agggtcatgg tagaatcaga
      361 gacgaacatg cggttcgttc ggcgcaacgt tcgttgagcc ggtgtcagac cggaccgggc
      421 agagaccagg acagccaatt gaagcgcgcg atcgattccc agaaccgacc gtcgagattg
      481 cgaacgcaac ggcttatgct tttcgctgcg ttcgttgtgg taagctcgtg gatggtcttc
      541 gtttcgtggc gaacccagaa gtctgaaagc gtccgtcgga aagttccggt ctcgccctac
      601 gagaacaccc gcccggaggt aaagtatctc ggggatccgg cgtgtgttgg ctgccacgcg
      661 caaagtgctc ttgccttcgg caagcacccg atgggccgct ccctcgcacc catcggcgag
      721 aactacataa agaagggcga cgagggagac gcccaaaccc tgttcgagag tcaaggcctc
      781 cagtactcca tcgaacatcg ggacggtcac gtctttcacc aggagaggcg acgcgatgca
      841 tcgggccgga ttatcgccaa gagcgaggcc gaggtccggt tcgcggttgg ctcgggccgg
      901 cagggatctt cctacctggt cgagcacgat ggctttcttt tcgagtcccc gcttacctgg
      961 tattcccgga agcagcaatg gggcctgtcg cctggttttg aagccatcga ctatcacttc
     1021 gaccggccga tccagcgcaa ttgcctgttc tgccacgcga atcgggtcga gccgagggaa
     1081 ggcacgatca accagtatcg acagccgatc ttccagggtc acgcaatcgg ctgtgaacgc
     1141 tgtcatgggc caggcgagct tcacgtcgca cgtcccacga tggtcgacgg taaggacatg
     1201 acgatcgtca atccggccga tctggagcca gcgctccggg atgacgtctg tgcgcagtgc
     1261 cacctggccg gtgattctcg gattgtgcgc gttggtcggc gaaatgaaga ctatcgacca
     1321 gggctgccgt tttttagctt ctggacggtg ttcgttcaac cgccggacca ggccgaagac
     1381 cgttttgtcg gccagtttga acagatgcag gagagcaatt gctttcgcgc cagtcgcggt
     1441 cggctcgggt gcatttcttg ccacaatcca caccattttc ccgcccccgc ggagaaggtt
     1501 gcgtattacc gggatcggtg tctcgagtgt cacgctgagc ggggttgcag ccttccagcg
     1561 agcgaccggt tggagcgcag cagcgacgat gactgcacca gctgtcacat gccgaaggcg
     1621 agcagcgttg acatccctca tgccgcgacg gcgaatcatc gtattctccg tcatgggggc
     1681 ggagggattc cactcacggt caatcccggc aaaactggcc gcgacagccg ccatctggtc
     1741 gttttccatc gcgagcggat ggatggcgac gagcgagccc tggttgagcg agaccgcgga
     1801 gtggccttgt gccgcggcgg agcggcgggt gcgcggtttg cactcccgtt gctcgagg
//
LOCUS       DATAQL010000665         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4953705, whole genome shotgun sequence.
ACCESSION   DATAQL010000665 DATAQL010000000
VERSION     DATAQL010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4953705"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VHS97_11615"
     CDS             complement(<1..460)
                     /locus_tag="VHS97_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX3448896.1"
                     /translation="MSDSLTPMMQQYRELKARDPDALLLFRMGDFYELFGDDAERASA
                     LLGLTLTSRDKGADALPMAGFPHPALDSYLAKIVQAGLRAAVCEQVEDSREAKGLVKR
                     DVVRIVTPGTLTDDELLDPRTANYLAAIVEVGGKLGLAWVELSTGQFSLTG"
     gene            complement(515..874)
                     /locus_tag="VHS97_11620"
     CDS             complement(515..874)
                     /locus_tag="VHS97_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3448897.1"
                     /translation="MSQGPRRHLRLENVDGVTVVSFVESKIVTEENIQEVGDQLYSLV
                     EDEGHKNVLLNFGNVQYLSSAALGKLINLKKKVAAVKGKLKLCCIHPDLLEVFRITRL
                     DQVFEIYADEQAALDKF"
     gene            complement(1189..2031)
                     /locus_tag="VHS97_11625"
     CDS             complement(1189..2031)
                     /locus_tag="VHS97_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3448898.1"
                     /translation="MKTRRATTRAVAFLLMSVSSAWGDDFGRLEGPAFFNLITGPGVR
                     AHASLSFRELEALPTILRDERAALVIVKTDQGNLAKILVSAGLRKLKPSQKDGPLVPV
                     LLLDRFETIDAGDRRSFTARGKEVMLFDGFQFDLDAGQVVPEGLGGDIVFSTRAPEDP
                     RLAALGESRLYTLDKPPPSPENAPSKPSGGRGVQPGDFAGRYFLMANGQWSGPLELNV
                     DDAGVVSGHFRSDRNGSSYPVTGKIAGEIAQKIVFTVQFPRARQDYEGFLWTEGKNAI
                     AGTL"
BASE COUNT          532 a          729 c          697 g          484 t
ORIGIN      
        1 ggccagtcag cgaaaactga cccgtcgaca gttcgaccca agcgagcccc agttttcccc
       61 cgacctcaac gatcgccgcc aggtaattgg ccgtcctggg atcgagcagc tcgtcatccg
      121 tcagggtccc gggcgtgacg attctgacaa catcacgctt tactaaacct ttggcctcgc
      181 gtgagtcttc gacctgctcg cacacggccg cgcgcagacc cgcctgtacg atcttggcca
      241 ggtaagagtc aagtgccgga tgaggaaacc ccgccatcgg caaggcatcg gctcccttgt
      301 cgcgcgaggt caacgtcagg cccaatagcg ctgacgcccg ttcggcgtcg tcgccgaaaa
      361 gctcgtaaaa gtcccccatg cgaaacagca agagcgcatc cggatcacgg gccttcagct
      421 cgcgatattg ctgcatcatc ggtgtcagcg aatcgctcaa tgagaccggt caatcctcgg
      481 gagaaaagtc tgcgggccga agagtagcgg ggaatcagaa cttgtctagg gctgcctgtt
      541 cgtcggcata aatctcgaag acctgatcga gccgggtgat gcggaagacc tcgagcaggt
      601 cggggtgaat gcagcaaagc ttcaatttgc ctttgaccgc ggccaccttt ttcttgaggt
      661 tgatcagctt gccaagcgcg gcactcgaga ggtactgaac attgccgaag ttcagtagga
      721 cgttcttgtg accttcatct tccaccaagc tgtaaagctg gtcgcccact tcttggatgt
      781 tttcctcggt cacgatcttg gattcaacga aactgacaac ggtcactccg tcaacatttt
      841 cgaggcggag atgacggcga ggaccttgcg acatcggggt taaactcctg gcgagaacgg
      901 gaaccgtacc gaatcggaat agtttaccac atgaggggcc ggtcccgcac gcaggcctct
      961 ctgttgtttt tgtacgagtt tctcagaaac gaatcgaccg aggccagaac ccagcgttat
     1021 gaccacactt accacaatac ctcaaggaat caagggagca ccgacaaaag gtgttcgggc
     1081 gtcgacagtt tcactgccag aacaacttcc gtttgcacat tacctatttt gaccaagcgg
     1141 cgaagttccc tcgcgtatgg cgataaagct atacggacga tcgagcatct agagcgtccc
     1201 ggcgatcgcg tttttgcctt ccgtccagag gaagccctcg taatcttggc gagcgcgggg
     1261 aaattgaacg gtgaagacga tcttttgggc gatctcgccg gcgatcttgc ccgtgacagg
     1321 atacgacgat ccgttgcggt ccgagcggaa atggcccgag acgacgccgg cgtcgtcgac
     1381 gttgagctca agcggtcccg accactggcc attggccatc agaaaatagc ggccggcaaa
     1441 atcgccgggc tggacaccac gcccgcccga cggcttgcta ggcgcgttct ccggggaagg
     1501 gggcggtttg tcgagagtat acaaccgaga ttcgcccaaa gcggccagtc gcggatcctc
     1561 aggcgctcgc gtggaaaaca cgatatcgcc tcccaggcct tcgggaacaa cttgtccggc
     1621 atcgaggtcg aattgaaacc cgtcgaaaag catgacctcc ttgccccttg ccgtgaacga
     1681 acgccggtcg ccggcgtcga ttgtctcgaa acggtccagc agcaacaccg gcacaagcgg
     1741 tccgtccttc tgcgacggct tgagcttgcg gagaccggct gatacgagga ttttcgccag
     1801 atttccctgg tcggtcttta cgatcacgag cgccgcgcgc tcgtcgcgaa gaatggttgg
     1861 cagcgcttcc agctcgcgaa aagaaagact cgcgtgggcg cgcacgcccg gtccggtgat
     1921 aagattaaaa aacgctggcc cctccagccg gccgaagtcg tcgccccatg cagacgatac
     1981 cgacatgagc aagaaggcca cagcccgggt cgtcgctcgc ctcgttttca tcacgaaagg
     2041 acgcctttcc cccacgttcg tgttccgcac cggaccgcgt cgcgagatcg cgcactgcct
     2101 atcgttgaga gtgcccagtc tctcggccac gcagtgctca atcgtctccg gctttgattc
     2161 aagttgcaac atagatcaag ccgatccagg cgatacgcca gacgattttc ggcaaagaaa
     2221 tggcaacggc acgcgaatct acgaatacca caccatgctg cgagcgtacc gcggaggggg
     2281 ttaacgatgg gtttggacct gacattagca gtcatcatcc tgatggccgc gctccgaggc
     2341 tggtttcagg ggttcgtcag tcaggcagtg cggctcggtg gcatcattgc atgcgtgtat
     2401 ctggccgagc cagtgcgcga ttacgccaag cccaatatcc tg
//
LOCUS       DATAQL010000666         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4038260, whole genome shotgun sequence.
ACCESSION   DATAQL010000666 DATAQL010000000
VERSION     DATAQL010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4038260"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VHS97_11630"
     CDS             complement(<1..431)
                     /locus_tag="VHS97_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448899.1"
                     /translation="MTDWVDLIIDPAASALASSVTPADFFDPSVFDAALTGLAAPGGW
                     ETLLSDLTSISSSGLVLPETSTAATDAGSAAAAADSSTAFWQGLEQSWINSSFGQQVD
                     TSLNTWFAQADPTALHAAADPTGGACGLICNGANGVGGGSL"
     gene            1021..1548
                     /locus_tag="VHS97_11635"
     CDS             1021..1548
                     /locus_tag="VHS97_11635"
                     /inference="COORDINATES: protein motif:HMM:NF004548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX3448900.1"
                     /translation="MTAPAVGAEDRSATLSKKVESTGLSGEAHGRISAELDRIRERRD
                     RLEVEVRNDRGAVSDHADAADAIQRADELASLDERIVELERLLRAGPAPSDGTGTLPF
                     GTEVKLRFSDGAVVKMQVISVIEETALGGEAEALTAGSPLGLALVGRKKGDKVTYTTP
                     QGENQVELLDVKYPK"
BASE COUNT          354 a          521 c          512 g          280 t
ORIGIN      
        1 ctcaacgaac cgccgcccac accgttggcg ccgttgcaga tcaaaccgca cgcaccgccg
       61 gtcgggtcgg cggctgcatg cagggcggtc ggatcggcct gcgcgaacca tgtgttcagc
      121 gaggtgtcca cctgctgacc gaacgagctg ttgatccagc tctgctccag gccctgccaa
      181 aacgcggtcg acgaatccgc cgccgccgcg gccgagccgg catccgttgc ggcagtactc
      241 gtttcgggaa gcactagccc ggagctggaa atgctggtca gatcgctcag cagggtctcc
      301 cacccaccgg gggcggccaa accagtgagg gcggcatcaa acacagaagg atcgaagaaa
      361 tcggccgggg tcaccgagct ggccaaagca ctcgcggccg ggtcgatgat caagtcgacc
      421 caatcggtca acgggtcagc cttcgcagtc ggcgcccccg ccagcggccc gagaccaagg
      481 gccaaaaatg cacctgcacc tgcacccaga cctgcggcac gcgtgcgacg gcgcgccttg
      541 gccttgcttg accgacgatt cttcttgctg tgctgtccag acatgacttg ccttcccacc
      601 cagaattgct ggaacccctc gctctcagcc ccggctaagg taactgttag gccaacttac
      661 gtccagaaaa ttagacaatt gattcaagat cgctttgaga atttaccgta ctcgggtcgg
      721 cagcaacctt gttcacgcac tagtgcgcag ttagccgtcc ttattcgcga gcggtagcaa
      781 ctttcggaaa cgtttgccgg aaaccggaat tttattgaca atctgcgtgt cgcgcctgag
      841 ctaagacgaa ttttctctca gtcccgtcac atatttagct agggtaccga tcaaaccctg
      901 ggcaaaacgg ccgttttagc caagaccact cagggcggac gaccgtcgtg caaaaacgtc
      961 atacaacccg caacgaacgc atgaataatc ggcttaagtg caatacagtc ttggcacctc
     1021 atgacggcac ccgccgttgg ggcggaagac aggtcggcga cattgagcaa gaaggttgag
     1081 tccacggggc tttcgggtga ggcgcatggg cgcatcagcg cggagttgga ccgaatccgg
     1141 gaacgacgcg atcggctgga ggtcgaggtc agaaacgacc gcggcgcggt gagcgatcat
     1201 gccgacgccg ccgacgccat ccagcgcgcc gatgaactgg ccagcctcga cgagcggatc
     1261 gtcgagctcg agcggctact gcgagccggg cccgcgccat cggacgggac cggaacgttg
     1321 ccgttcggca ccgaggtgaa gctacggttc tccgacggcg ccgtcgtcaa gatgcaggtg
     1381 atctcggtga tcgaagagac agccctcggc ggcgaagccg aggcgctgac cgcgggcagc
     1441 ccgttgggtc tggcgctggt cggacgaaag aagggcgaca aggtcaccta cacgacaccc
     1501 cagggcgaaa accaggtcga gctgctcgac gtgaaatacc ccaagtagtc cgccgtagaa
     1561 tttcggccga tggccgagat cgcggcaagc acgcagctcc cgctcaagac gcaggtgtgg
     1621 cggttcattg tcaccggcgg actgtcggcg atcgtcgact tcggcct
//
LOCUS       DATAQL010000667         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3121813, whole genome shotgun sequence.
ACCESSION   DATAQL010000667 DATAQL010000000
VERSION     DATAQL010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3121813"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VHS97_11640"
     CDS             <1..245
                     /locus_tag="VHS97_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448901.1"
                     /translation="AFAIIVAAWWWDASLLTAAADANLQLIKIVTSKLPFDWGSKVES
                     ALQIFGADRALLLIEGVAIAKVVMLGFAYPFRRRRR"
     gene            650..835
                     /locus_tag="VHS97_11645"
     CDS             650..835
                     /locus_tag="VHS97_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448902.1"
                     /translation="MSDQLASWIGDMIAAAGLILPIVQFFRKKPTQRHKRVRRLRHWK
                     IGGIERTRLDEIDDRQL"
     gene            1069..1791
                     /locus_tag="VHS97_11650"
     CDS             1069..1791
                     /locus_tag="VHS97_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448903.1"
                     /translation="MQVVGEQGSIAHRLNKLMVLVLDELSPDYAVDDGRLLRDALVED
                     AIARLPGYGYGSEDPGEDTSYLPPRAAAFLRALKLNGFTVSGGELRRALPVDVGLPTA
                     QSEVDRLLEKHRLATPKGHLKEALDAHGRGSWAAANSQIRTFLDSLLDEVAVKLDPTA
                     ATMGSGQPRRTKLASQGFLSRELNEWDDNGLGFINGLAKRLHPKGSHPGLSDDEDSTF
                     RLHVVLLTARLLLTRFDTWGRA"
     gene            1788..>1980
                     /locus_tag="VHS97_11655"
     CDS             1788..>1980
                     /locus_tag="VHS97_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448904.1"
                     /translation="MNQLVPITTPRLPELVAAAGDLASMRFLEFFAANIRNPHTRRAY
                     ARAAEEFLAWCADAGVPSIA"
BASE COUNT          383 a          578 c          593 g          426 t
ORIGIN      
        1 gcgctttcgc cattatcgtc gcggcctggt ggtgggacgc gtccctcctc acggcggctg
       61 cggacgcaaa tcttcagctc atcaaaatcg tcaccagcaa attgccgttt gactggggga
      121 gcaaggtcga aagcgcgctg caaatcttcg gcgccgatcg cgccctcttg ctcatcgagg
      181 gcgtcgccat cgccaaagtc gtcatgctcg gattcgctta ccctttccgc cgccgtcgac
      241 ggtaaaagca aaactgcatg cgctactttc cagagagttg gtaaccgctc tggcttcacc
      301 atctcggaca agtctgttgt gcaatagacc ctcaaccaaa agtcgctgtc tggatgttct
      361 ggaaaatcag cgcgacggga gcagggattc caagtcgtag ggatcggctc gattcgcctg
      421 ttaggggaga ttggaagggc agaagaacga ccgagagcgg ccgtttccgg acattgccga
      481 gaatgaattg accgatcgga attatatacg cacttttatg caatggtgcg ataggtgact
      541 ttcaagcacc tgcctgccga aagtgtttgg caggtctgta ctccgtagag gcccctggcc
      601 tcgtttgcga cccgcccttg tggcgggttt tgtttaggca ccacctcgaa tgtctgacca
      661 gcttgcgagc tggatcggcg acatgatcgc ggctgcgggc ctgattctcc cgatcgtcca
      721 gttcttcagg aaaaagccga cccaacgtca caagcgggtg cggcgactcc ggcattggaa
      781 aatcggcggg atcgagcgca cgcggctcga tgagatcgac gaccgccagc tctagagcta
      841 cgaccctcgc tcatacaccc ttagggggcg gcgcgccgcc cctttgaccc ccattctcgc
      901 tttgaagtgc tatatgtgat aatgcccttt atcgaatact ttctagaggg ttaggctatg
      961 cccgatggtc ggttctcgcg ttcgacggtc atcacagcaa tcgagacgat ggagtcattt
     1021 actcacgcgg agctgacacg ctcccttatc aaattgaggc cggacctcat gcaggtggtc
     1081 ggtgagcaag gcagcatcgc tcatcggctc aataagctga tggtgttggt gcttgatgag
     1141 ttgtcccctg attacgccgt cgacgacggc cggttactgc gggacgctct cgtggaggac
     1201 gccatcgccc gactgcctgg gtacggatat ggctccgaag atccgggcga ggatacatcc
     1261 tatttgcccc cgagggcggc ggcgttcctg cgcgccctta agctcaacgg gttcacggtg
     1321 tcgggcggag agctgcggcg ggcattgccg gtcgatgttg gcctgccgac ggcacaaagt
     1381 gaggtggatc gtttactcga aaagcatcgg ctcgcgacac cgaagggcca cctgaaggaa
     1441 gctcttgacg ctcatggccg cggcagttgg gctgccgcga acagtcaaat ccgcaccttc
     1501 ctggattctc tgcttgatga ggttgctgtc aaacttgatc cgacggcggc gaccatgggc
     1561 agcggtcagc cgcgacggac gaaacttgct tcgcaagggt ttctcagccg ggaattgaac
     1621 gagtgggacg ataacggtct cggtttcata aacggcttgg caaagcgtct ccaccccaag
     1681 gggtcacacc ccggcttgtc cgatgatgaa gacagcacct ttcggctgca cgttgtgctg
     1741 ctgacggcgc ggttgctgct gactcgtttc gatacatggg ggagggcatg aatcagctcg
     1801 tcccgatcac tacgccacgc ctgcccgagc tcgtcgctgc ggccggcgat ctcgcctcga
     1861 tgcgcttcct cgaattcttc gcggccaaca tccgcaaccc gcacacgcgc cgagcctatg
     1921 ctcgagctgc agaagaattt ttggcctggt gcgccgatgc cggcgtgccg tcgatcgcgg
//
LOCUS       DATAQL010000668         9927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3733305, whole genome shotgun sequence.
ACCESSION   DATAQL010000668 DATAQL010000000
VERSION     DATAQL010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733305"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VHS97_11660"
     CDS             <1..651
                     /locus_tag="VHS97_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX3448905.1"
                     /translation="SMMDLVECLICGAIEALGGGFARPWGEVTVDFTPPWPRRTYAEL
                     LAQHAAVDPTDFAAVKARAEAVGLATAGKDPDVVANELFEATVEDHLEGPIFVIDYPA
                     AMCPLTKRKASDQAVAERFELFVRGVELANAYTELNDPRLQEALFRKQLAGLAAEDSM
                     AKMDDDFVRALKHAMPPAGGVGIGIDRLCMILLNRTSIRDVILFPLMRPQSGGTTA"
     gene            708..2132
                     /locus_tag="VHS97_11665"
     CDS             708..2132
                     /locus_tag="VHS97_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3448906.1"
                     /translation="MYKYLLCWRYLRTRYIALASVISVMLGVATMIVVNSVMAGFAEK
                     MRDRLHGVLADVVVESVSLDGFYNSEDVMARIKQVAGDQVIAQAPTMETPGILHFRFP
                     GGQVITRPVQIIGVRPEDRAKTGDFGEFLVDDQNRKIAPSFEVTDKLRQETPAGQILK
                     DLPKSSDDPFEKLMEEQLKKEALEQAPDDGTIIGYAIATHHRGKNLPDHFIAPPGTKV
                     GLLFPMAGKVRSEGGFDTFTVVGYFKSGMSEYDSTHVYVPLERLQDQRRLRDVNGRGA
                     VNQIQIKVNPGVALDLLAEKLQVALDELHPAFFRVYTWEQKQGPLLGAVAIEQSILNI
                     LLFFIIAVAGFGILAIFSMIVVEKTRDIGVMKALGASTAGIRGIFLGYGLLLGVVGSG
                     VGMAGGLLFVRYINEVERFLSSVTHRKVFDDTIYYFDKIPTVVQPFTVFWIVTGALLI
                     AVGASIWPAQRAAHLQPVRSLRYE"
     gene            2206..2961
                     /locus_tag="VHS97_11670"
     CDS             2206..2961
                     /locus_tag="VHS97_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3448907.1"
                     /translation="MSAQLTARGLFKSYWKGKNEVQVLRGIDIEVARGEMVAVVGASG
                     SGKSTLLHLLGLLDAPDNGEVLFEGQRIDNRGERQRDQLRNETFGFIFQFYHLLPELT
                     ALENVLMPQLIRHGVLSYWGERRRLKKEGTELLERVGLGHRLTHRPAELSGGEMQRAA
                     IARALAGRPEVLLADEPTGNLDAANGHGVLELLRDLNHERGLTMILVTHDQQIAQQAD
                     RVVRLIEGRIHASSPTWPDFPDDAEAGQSASAA"
     gene            2986..3855
                     /gene="ilvE"
                     /locus_tag="VHS97_11675"
     CDS             2986..3855
                     /gene="ilvE"
                     /locus_tag="VHS97_11675"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEX3448908.1"
                     /translation="MSPSPKVYISGKLYDKADAKVSVFDHGLLYGDGVFEGIRAYSGR
                     VFRLKQHVDRLYESAQGIHLQIPIKREEMASAINDTLAVNKLSDAYIRAIVTRGAGSL
                     GLDPRKTTDPQVIIITDHISLYPEELYDHGLKIITAATTRNHPNAVNPRIKSLNYLNN
                     ILAKIEGTNAGCLEALMLNHKGEVAECTGDNIFVVHGGRIHTPSSDSGILEGITRAAV
                     IELARAAGHTVIERTMDRHDVFNADECFLTGTAAELIPVVECDGRVIGAGRPGPVTRE
                     LRQRFQALVREER"
     gene            3870..4346
                     /locus_tag="VHS97_11680"
     CDS             3870..4346
                     /locus_tag="VHS97_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3448909.1"
                     /translation="MIEIKSILAPTDFSAHSEQAVRYACRLAERLDSELHLLHVLSEI
                     VPAGPDPLLMPMMPAQFYKESEDRAQETLDHSLDPSWGKPAKIITAVRWGSPVESIVS
                     YAVDHRIELVIISTHGRTGLSHVLLGSVAERIVREAPCPVLTIRDRSGARPVVPTT"
     gene            4423..4980
                     /locus_tag="VHS97_11685"
     CDS             4423..4980
                     /locus_tag="VHS97_11685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3448910.1"
                     /translation="MNDPHPQRRLIYRGLKIDLALQSVPLADGTAADREVIIHRGAVA
                     LVPMVDDEHVCLVLNHRYSIGKTLLEVPAGTIDEGESPEQTAMRELHEETGYRAGRIR
                     HVRDWYVSPGVMTERMYLFLCEDLEAGLAQHELDERLENSVVPWNDALAMIDDGTIED
                     AKTILALSICDRLRRKRNDQGPPQG"
     gene            complement(5046..6005)
                     /locus_tag="VHS97_11690"
     CDS             complement(5046..6005)
                     /locus_tag="VHS97_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448911.1"
                     /translation="MNAEEMIEYVLCESKTLELQEHDAALHVDSAQEVRLQCLRVAIA
                     RLVDDGTELEYPPGLARRTVAFVARNRRRPFSILDYVPVSLPFRWADFAVAASIFIAG
                     TLTLLPAIQRSRERMNQAGCVFNLQQMGQSLAEYSTLHRTLPYPPSHRADTHAGMFAV
                     ILHDAGVLNNLSVLDCPCNGRSPHGFKELASFEQVDQIRRTQPDAYRRMLIWDYAYNV
                     GYRHPSGHNGPLEAVHSSRIPVLADQPDHEDFLTIREGNSPNHGRRGQNVLFGDGSVL
                     WFHSRRVGPSDPDLYLNNEEQPRPGVHVLDSVLVPSKVPFGGR"
     gene            complement(6010..6588)
                     /locus_tag="VHS97_11695"
     CDS             complement(6010..6588)
                     /locus_tag="VHS97_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3448912.1"
                     /translation="MSRYREDGRAALFSELVHRYERELYRYLARYLGDPTGAEDVFQN
                     TFLQVHLKRGLFENGRPFRPWLYAIATHQAVDALRKAGRHPTVSLDQRINSTRTEGDA
                     GNLIDLLVNDNVGPLAELQEAERQQWVRDSIARLPDALRQTLILAYHQDLKYREIAEI
                     LKIPVGTVKSRLHAALEKLQQMARSVKRDGDG"
     gene            complement(6846..8492)
                     /locus_tag="VHS97_11700"
     CDS             complement(6846..8492)
                     /locus_tag="VHS97_11700"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX3448913.1"
                     /translation="MVFLCFLIWSLEAEGFVIPTGSMAPTLMGRHKEIACPQCGYLFT
                     VNADREVDPTAKGESARRRIRSGTCENCRFEASVEESPSFSGDRIYVMKDGLSLPFFG
                     ARGRVKLKRWDVAVFKLPEEPEVRYIKRLVGMPNEVIRIDGGDLWVQPLDRSREFERL
                     RRPPDHQEAMQLLVFDDTHRAKAIAEDPRWLRWVAPSGCDWPQSLPGVYSPHDHTKGW
                     TELRYHHVVPSPAQWDAIRAGKPLPRPPFSTLITDFYSYNTDVSLYDQSHPASSARAW
                     FQPHWVGDLTIALRLTVRERIGQFRMELIKAGVSNRCELDLASGLARLYHGESPLGDA
                     VLTEMTRDGTYDVVFANVDGRLTLWVNGRLPFGDGRTYESSLESAAPTAADLEPARVA
                     ARGAAIKVERLVLRRDLYYTLEPAESDYSNLDGSTRIESSALLELLSDPARFPGLTRY
                     PARDYSIGPLRYLMLGDNSPWSRDGRAWGSSDQIDPEIPGQGWDISGRASWEVPEALL
                     IGKAFCVYWPHPKPVWPKLSLGPDIRLPIRPYIEQMRWIR"
     gene            8858..9388
                     /locus_tag="VHS97_11705"
     CDS             8858..9388
                     /locus_tag="VHS97_11705"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX3448914.1"
                     /translation="MESIPTSHKDLGPAANWPARGSDLTIHRALMAVLWTIVIMMLCW
                     LPRQVVQRVEAGSGWFKIVNLDKFVHGGIFIVFALLWARVWSSPRRFAWIALGGVIFA
                     AITEIGQLIPAVGRDATVPDLIADSIGVLIGILAFPWAEPWFQLIESRLFPESRSYSD
                     LRTRPNATTDKVPSSR"
     gene            9410..>9927
                     /locus_tag="VHS97_11710"
     CDS             9410..>9927
                     /locus_tag="VHS97_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448915.1"
                     /translation="MSGVRANVLSRSITVGVCWAGLVGTPLQAGEPMRPLSVEADNQG
                     LRASPTKKLIEFGWDEPDTGMLQRHRDQFERSPFDGCVFHVATRDAKGTPENFTWLCW
                     GRRRFTDLEIKPAHNELASITWTRFRHNFLRFNVTPGDLDWFEDHAAVLANARLAAQV
                     ARAGHCDGILLDT"
BASE COUNT         2039 a         2936 c         2936 g         2016 t
ORIGIN      
        1 tccatgatgg acctggtcga gtgcttgatc tgcggcgcga tcgaggcgct cggtggcggg
       61 ttcgcacggc cgtggggcga agtgacggtc gatttcacgc ccccctggcc gcgccggacc
      121 tatgccgagt tgctggctca acatgcggcg gtcgatccca cggatttcgc ggcggtcaag
      181 gctcgtgccg aggctgtcgg actggcgacc gcgggcaaag acccggacgt cgtcgccaac
      241 gagttgttcg aggccaccgt cgaggatcac ctcgaaggtc cgatcttcgt catcgattat
      301 cccgccgcaa tgtgcccctt aaccaagcgc aaggcttccg accaggcagt ggccgagcgg
      361 tttgagctct ttgtacgggg ggtcgaactg gccaatgcct acactgagct gaacgatccg
      421 cgcttgcagg aggctctttt ccgcaagcag cttgcaggtt tggcggccga ggattcgatg
      481 gccaaaatgg atgacgactt tgtccgcgcg ctcaagcacg cgatgccgcc ggcgggcggc
      541 gtcggtatcg ggatcgaccg gctctgcatg attctcttaa atcgaacaag cattcgcgac
      601 gtgatcttgt ttccgctcat gcgcccgcaa tccgggggga caacggcctg acgctcgctg
      661 cgggtgcttt cgcctttcgc cgataaccca tggaaggaac cgcataaatg tataagtatt
      721 tactctgctg gcgatatttg aggacccgat atatcgcgct tgcgagcgta atcagcgtga
      781 tgctcggcgt cgcgaccatg atcgtggtca actcggtgat ggccggcttc gccgaaaaaa
      841 tgcgcgaccg gctgcacggc gttctggccg acgtggtggt tgagtcggtc tcgctcgacg
      901 gcttttacaa ctcggaagac gtgatggcgc gaatcaaaca ggtcgccggt gaccaggtga
      961 ttgctcaggc gccgaccatg gaaacgccgg gcatcctcca ctttcgcttt cccggcgggc
     1021 aggtcatcac gcgtcccgta cagatcatcg gggtgcggcc cgaagatcgg gccaagaccg
     1081 gcgactttgg tgagttcttg gtcgacgatc agaatcgaaa aatcgccccg tcgtttgagg
     1141 tcaccgacaa gttaaggcag gaaacgccgg ccggccagat cttgaaagat ctccccaaaa
     1201 gctctgacga cccgttcgag aaattgatgg aagagcagct caagaaagag gcgctcgagc
     1261 aggcgcccga cgatggcaca atcatcggct atgccatcgc gacgcaccat cgcggcaaga
     1321 acctgcccga ccacttcatc gcgccgccag gaacgaaggt cggtcttcta tttccgatgg
     1381 ctggcaaggt caggtcggag ggcgggttcg atacgttcac ggttgtcggt tacttcaaga
     1441 gcggcatgag cgagtatgat tcgacacacg tttatgtgcc actcgaacgc ctgcaggatc
     1501 agcgtcggct gcgcgacgtg aacggccggg gagcggtcaa ccaaatccag atcaaggtaa
     1561 atccgggcgt tgctctcgac ctcctggccg aaaagctgca ggtcgcgctc gatgagcttc
     1621 accccgcatt tttccgcgtt tacacctggg aacaaaagca aggaccgctc ctgggggccg
     1681 tggccataga gcaaagcatc ctgaacattt tgctcttctt catcatcgcc gtggccggct
     1741 tcggcattct ggcgatcttt tcgatgatcg tcgtcgaaaa gacgcgtgac atcggcgtca
     1801 tgaaggctct gggagcctcg accgccggaa tccggggcat ctttctcggc tacggtctct
     1861 tgctgggtgt ggtcggcagc ggtgtgggga tggctggagg gctactcttt gtccgctata
     1921 tcaacgaggt cgagcgtttc ttgagctcag tcactcaccg caaggtattc gacgacacga
     1981 tctactattt cgacaaaatc cctacggtgg ttcagccgtt cacggtcttc tggatcgtga
     2041 ccggagctct cttgatcgcg gtgggggcca gcatttggcc ggcacagcga gcagcccatc
     2101 tgcagccggt ccggtcgcta cgttacgaat gagctgactc taacttgcgt gcgggcgcgg
     2161 aaacaggtcc gatccactct tctacttgca tggaaggcat aaagaatgtc ggcccaattg
     2221 acggcacgtg gtcttttcaa gagttactgg aaaggcaaga acgaggttca ggtgctgcgc
     2281 gggatcgaca tcgaagtcgc ccgcggagag atggtggcgg tggtgggcgc cagcggctcg
     2341 ggcaagagca cgctcttgca cctgctcgga ttactcgacg cgccggataa cggcgaggtc
     2401 ttgttcgagg gccaacgaat cgacaaccgc ggtgaacgcc agcgcgacca gcttcgcaac
     2461 gaaactttcg gcttcatctt ccagttctac catttgttac cggagctgac ggcgctcgag
     2521 aacgtactga tgccgcagct gattcgccac ggtgtattat cgtactgggg agagcggcgg
     2581 cgactgaaga aagaagggac tgagctgctg gaaagagtcg ggctcgggca ccggctgacc
     2641 catcgcccgg ccgagctctc cggcggtgag atgcagcggg ccgcgattgc ccgcgcactg
     2701 gctggccgac ccgaggtctt gctggccgac gagccgaccg gcaacctgga cgccgccaac
     2761 ggccacgggg tcctcgaact attgcgcgac ttgaaccacg aacgaggtct cactatgatt
     2821 ttggtaacac acgatcagca gatcgcgcag caggccgacc gggttgttcg gctcatagaa
     2881 ggtcgaattc atgcgtcatc tcccacctgg ccggattttc cagacgacgc ggaagccggg
     2941 caatcggctt ccgccgcgta acgtcgaagt gagaggaccg cgcggatgag cccgagcccc
     3001 aaggtctaca tcagtggcaa actgtatgac aaggccgacg ccaaggtgag cgttttcgat
     3061 catggtctgc tctacggcga cggcgtcttc gaggggatca gggcttactc cgggagagtg
     3121 ttccggctca agcaacacgt cgatcgattg tatgaatcag cccagggtat tcatctgcag
     3181 attccgatca aacgagaaga gatggcgtcg gcgatcaacg ataccctcgc cgtcaacaaa
     3241 ttgtccgacg cctacattcg cgcgatcgtc actcgaggcg ctggcagctt gggtctcgat
     3301 ccgcgcaaga cgaccgaccc tcaggtcatc atcatcaccg accacatcag cctctatccg
     3361 gaagagctgt acgaccacgg attgaagatc atcacggccg cgacgactcg taatcacccc
     3421 aacgctgtca acccccggat caagtcgctc aactacttga acaacatcct ggccaagatc
     3481 gagggaacca acgccggttg cctcgaggcc ttgatgctca atcacaaagg cgaagtggcc
     3541 gagtgtaccg gcgacaacat cttcgtcgtt catggcggga gaattcacac accctcgagc
     3601 gactcgggaa tcttggaggg tatcacccgg gcggccgtca tcgaactggc gcgcgctgcc
     3661 ggacacacgg tgatcgagcg gacgatggac cgtcatgacg tgttcaacgc cgatgagtgc
     3721 tttctgaccg gaaccgccgc cgagctgatc ccggtcgtcg aatgcgacgg gcgtgtgatc
     3781 ggcgccggtc gtccgggacc ggttacccgc gagcttcgtc agcggtttca ggcattggtg
     3841 cgcgaggaac gataaatgaa agtcgccacg tgatcgagat caagtccatc ctggctccca
     3901 ccgatttcag cgcccattcc gagcaggcgg tccgctatgc ctgccggctg gccgagcgac
     3961 ttgattccga gctgcacctt ctacacgttc tctccgaaat cgtgccggcg ggtcctgacc
     4021 cgctcttgat gcccatgatg ccggcccagt tttacaagga aagcgaagac cgggctcagg
     4081 aaacccttga ccactcgctc gacccctctt ggggaaaacc ggcgaaaatc atcacggcgg
     4141 tgcgctgggg tagtcccgtc gagtcgatcg tttcgtatgc tgtcgatcat cgcattgagc
     4201 tggtcatcat ctccacccac ggacggaccg ggctaagcca cgtgctgtta ggctcggtcg
     4261 ccgaacgaat tgttcgtgaa gctccctgcc cggtgcttac gatccgcgac cgatcgggag
     4321 cgcgcccggt cgtgcccacc acttaagacc cggatctcgc tgctgccggc gccgcacctt
     4381 caacctcgac gacgcaggat cagaccgagc tggagtaatc ccttgaacga cccgcatcca
     4441 caaaggcggc taatttacag ggggctgaag atcgatctag cgctccaatc agtcccgctt
     4501 gccgatggca cggccgccga ccgagaagtg atcatccacc gcggagccgt cgctctcgtg
     4561 cccatggtgg atgacgagca cgtttgcctc gtgctaaacc accgctattc gatcggtaag
     4621 acgctgctcg aagtcccggc cggcacgatt gacgagggag agtctcccga gcagactgcc
     4681 atgcgtgagc tccacgagga gacgggctat cgcgccggga gaatccgaca cgtgcgcgac
     4741 tggtacgtat cgccgggtgt catgaccgag cgcatgtatc tgtttctgtg cgaggatctc
     4801 gaagcgggcc tcgcccagca cgagctcgat gagcggcttg aaaactcggt cgtcccgtgg
     4861 aacgacgcgc tcgccatgat cgacgatggc acgatcgaag atgccaaaac catcctcgcc
     4921 ctgagcatct gtgatcggct caggcgaaag cgaaatgacc aaggcccgcc tcagggatga
     4981 tccccggatt ctcgcgggcc tcggggtcgg tcgtggagga agccgctgtc ggcctatggc
     5041 gcctgtcaac ggcccccaaa gggtacctta ctggggacca aaaccgagtc gagcacatgc
     5101 acgcccggac gcggttgctc ttcgttgttg agatagagat ctgggtcgct gggaccgacg
     5161 cggcgcgagt gaaaccacag tacgctgccg tcgccgaaga gcacattttg accccggcgg
     5221 ccatggttag ggctgttgcc ttcacgaatc gtgagaaagt cttcatgatc aggctgatcg
     5281 gcaagtacag gtatccgtga cgagtggacg gcttcgagtg ggccgttgtg tcccgaggga
     5341 tgccggtaac cgacgttgta ggcgtaatcc caaatcagca tgcggcgata agcatcgggc
     5401 tgcgtccggc gaatttgatc gacttgctcg aagcttgcga gctctttgaa accgtgagga
     5461 gatctgccat tacacgggca atcgaggacc gagaggttat tgaggacacc cgcgtcatga
     5521 agaatcaccg cgaacattcc ggcgtgagtg tcggcccggt gtgacggcgg gtacggcaag
     5581 gttcgatgca gcgtcgaata ctccgccaag ctttggccca tttgctgcag gttgaacaca
     5641 cacccggctt gattcatgcg ttcgcgcgaa cgttggatcg ccggtaaaag agtcagcgtg
     5701 cctgcgatga agatgctggc ggccacggcg aaatccgccc agcggaatgg caggctgacg
     5761 ggcacataat ccagtatgct gaacggccgt cggcgattgc gtgcgacaaa cgccaccgtc
     5821 cgccgagcca gaccaggagg atattcgagc tcggtgccgt cgtcgaccag tcgcgctatg
     5881 gccactcgaa ggcattgcag gcgaacctcc tgtgcagaat ccacatggag agccgcatcg
     5941 tgctcctgga gttccaaagt cttggactcg cacaggacat actcgatcat ttcttcggcg
     6001 ttcatggcgt catccatctc catcacgctt gaccgaccgt gccatctgtt gcagcttttc
     6061 gagcgcggcg tgtaagcgcg acttcaccgt tccgaccgga atcttgagga tctcagcgat
     6121 ttcacggtat ttcaagtcct ggtgataagc caggatcaaa gtttgccgaa gagcatccgg
     6181 gaggcgagca atgctgtctc gcacccattg ttgcctctct gcctcttgaa gttcagccag
     6241 tgggccgaca ttgtcgttga ccagcaaatc gatcaagttg ccggcgtcgc cttcagtgcg
     6301 tgtggagttg atccgttggt ccaggctgac cgtcggatgc cggccagctt ttctcagagc
     6361 atcgacagcc tgatgagtgg caatcgcata gagccaaggg cgaaacgggc gaccattctc
     6421 aaacaatcct cgctttaaat ggacctggag aaacgtattt tgaaacacgt cttccgcgcc
     6481 agtcggatcg cccagataac gggccaggta acggtacagt tcccgttcat agcgatgaac
     6541 gagctcgctg aacaaggccg ctcgcccatc ctcgcgatat cggctcatca gttcctcgtc
     6601 agaaagctga ctgaggtctt ccgccgaatc ggagccaatg gccttaacca tcttacgcac
     6661 cgccgcggcc aaaggttcag ggggtccgcg gccaaccaaa gctcgcgggg gctattggtc
     6721 gcgtcctggt tgatggtaca aaatcgtaac agtaagtcta agtacgtctt ctggcgagtc
     6781 aagggccatg tctacgacat gtttcggcta tctcggcctt gcgattaacc cacgattcgc
     6841 cgcttttagc gtatccagcg catctgttca atatacggac ggataggtaa tcgtatgtcg
     6901 ggacctaatg agagcttggg ccagactggc ttgggatggg gccagtagac gcaaaacgcc
     6961 ttgccgatca agagcgcttc gggaacctcc cagctcgccc ggccagagat gtcccagcct
     7021 tggccgggta tttctgggtc gatctggtcg gagcttcccc aagctcgtcc atcgcggctc
     7081 caggggctat tgtcgcccag catcaaatag cgcaagggcc cgattgaata atctcgtgct
     7141 ggatagcgag tcaggccagg gaaacgtgcc gggtcggaga gcagttccag cagtgcagac
     7201 gactcgattc gcgtggaccc gtccaggttg gaatagtcgg actcggccgg ttcgagcgtg
     7261 tagtagaggt cgcgtctcaa gaccaacctt tccaccttga tcgccgcgcc gcgtgcggca
     7321 acgcgagctg gctcgagatc ggcggccgtc ggagctgccg attcgagcga ggactcgtat
     7381 gtccgcccat ctccgaacgg gagacgaccg ttgacccaaa gagtcaagcg accatcgacg
     7441 tttgcgaaca cgacgtcata cgttccgtcc cgggtcattt ccgtcagaac cgcgtcgccc
     7501 aaggggctct caccgtggta gagccgcgca aggccgctgg ccaagtcgag ctcgcaacga
     7561 ttggaaacac ctgccttgat cagctccatc cgaaactggc cgatccgctc gcgcacggtc
     7621 aaacgcagcg cgatcgtcaa atcgcccacc caatgcggtt gaaaccacgc gcgcgccgag
     7681 ctggccggat gagactgatc gtaaagagac acgtcggtat tgtacgagta aaagtccgtt
     7741 atcaaagtcg aaaacggcgg gcgaggtaac ggcttcccgg cgcgaatcgc atcccactgc
     7801 gcggggctgg ggacgacgtg atgataccgc aactccgtcc accctttggt atggtcgtgg
     7861 ggggaataca ctcccggcag ggactggggc cagtcgcatc cggacggggc gacccagcga
     7921 agccagcgcg gatcctctgc gattgccttg gcgcgatgtg tgtcgtcaaa caccagcaac
     7981 tgcattgctt cttgatgatc gggcggccgc cgcagtcgct cgaactcgcg ggaacggtcc
     8041 agcggttgga cccataggtc gccgccatcg atgcggatca cctcgttggg catgccgacc
     8101 aaccgcttga tgtaacggac ctcgggctcc tccgggagtt tgaagacggc cacatcccat
     8161 cgcttgagtt tgacccggcc cctcgcgccg aaaaacggca aggacagacc atctttcatc
     8221 acatagattc gatcacccga gaagctaggc gactcttcca cggacgcttc aaaccggcag
     8281 ttttcgcatg tccccgaccg gatccgccgg cgcgcactct cccccttggc cgtcggatct
     8341 acttcgcggt cggcgttgac ggtgaagaga taaccgcact ggggacaggc gatttcctta
     8401 tgccgtccca tcaaggtcgg agccatcgat ccagttggga ttacaaaccc ttccgcctcc
     8461 agactccaga tcaggaaaca aaggaatacc accacgaatg actcgatcga ctctcgcccg
     8521 cttctggact tattgctccc ggttcttggg tgtgtctgag ttcgattcag gctcttcatc
     8581 agcatcctct gtttggtcga tgtcctcata gtgcagatcg catcacattc aaaataacaa
     8641 accaaggtcc cgaccaaaac cttgtcttgt aaacgaagtc tcattcctgt tgttcaaata
     8701 ataatgtttt attataaatg tgacctgact tcgcttgcga cttgaacgtt gtccgacccg
     8761 tttcaagcct gaattccctc gccaatttgg ccggaaacac ggtaggatga gtggccaacc
     8821 cgacttgaat atgaccctac cgaggagtga gctcatagtg gaatcaattc cgacgagtca
     8881 caaggatctc ggcccggcgg cgaactggcc cgcacgcgga tccgacctga cgatacatcg
     8941 cgccctaatg gctgtcctct ggacgatcgt gatcatgatg ctttgttggc ttccacgcca
     9001 agtggtccaa agggtggagg ccggctccgg gtggttcaaa attgtaaatc tggacaagtt
     9061 cgtccacggc gggattttca tcgttttcgc gctcttgtgg gctcgtgtct ggtcgtctcc
     9121 gcgacgcttt gcctggattg cgctcggcgg agtcattttt gctgccatca ccgagatagg
     9181 tcagctaata ccggccgtag ggcgcgatgc aaccgttccc gacctgatcg cggattccat
     9241 tggggtcttg atcggcattc tcgcatttcc gtgggcggag ccttggttcc aacttatcga
     9301 gtcgcgattg ttcccggaaa gccgctccta ctccgatcta cgtacgcgac ccaatgccac
     9361 gactgacaag gttccgtcgt ctcgatgaca acggcaacgg agcaccagaa tgtcgggtgt
     9421 gagagcgaat gtgctttcgc gttcaatcac tgtcggcgtt tgctgggctg gcttggtggg
     9481 cacgccgctc caggctggcg aaccgatgag gcccttgtca gttgaagccg acaaccaagg
     9541 tttgcgcgca tcgccaacga aaaagctcat cgagtttggt tgggacgagc cagacactgg
     9601 catgcttcaa aggcaccggg accaattcga gcggtcccca ttcgacggtt gtgtctttca
     9661 tgtcgccact cgcgacgcca aggggacgcc cgaaaatttc acgtggctct gctggggacg
     9721 ccggcggttc accgatctag agattaaacc tgcccataat gagctagcct cgatcacctg
     9781 gaccaggttt cgccacaatt tcctgcgttt taatgtcact cctggcgacc tcgattggtt
     9841 cgaggaccac gcagccgtgc tcgctaacgc ccggttggcc gcccaagtcg cccgcgctgg
     9901 ccattgtgac ggcatcttgc tcgacac
//
LOCUS       DATAQL010000669         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4648560, whole genome shotgun sequence.
ACCESSION   DATAQL010000669 DATAQL010000000
VERSION     DATAQL010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4648560"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VHS97_11715"
     CDS             <1..839
                     /locus_tag="VHS97_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448916.1"
                     /translation="ILLEDKYVAFGVSSDSVRAALTAVRRKDWKPSSSLEKACEHVPP
                     NLVILSVTDVSESLSSMLASLPGTLQTMINTSIALAKARASGTGTGTNAAPGNGRQPG
                     GPAGMPAGGPTSGGSGSMRAMMRPGGPGGASRPGFGPQANSGSSSPGGTAGDAASGTT
                     TENMIVLKVDPDKLPSAADLKAHLFPTTLSVTVSDPEIRVVTRGAFPDLSLPLGIVSA
                     GSMMPFLKPLIDGQPQAQAGGASGTQAGAPATTPAAGAPAAGAGTLKGRMRGGRGRGG
                     PE"
     gene            986..1438
                     /locus_tag="VHS97_11720"
     CDS             986..1438
                     /locus_tag="VHS97_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX3448917.1"
                     /translation="MEFVPEVTAFLENKPGRLAKICSALAQEKIDIRALSVMETDGPS
                     VLRFITSELEQTKNVLTSLGTEFRIAEVLAVQMENRTGSLARVLERLAAENINVEYAY
                     ASTTTAPRKALGIFHTSNPKRASQILNEAGAALAGGARSAGRRPLHTR"
     gene            1511..2674
                     /gene="rsgA"
                     /locus_tag="VHS97_11725"
     CDS             1511..2674
                     /gene="rsgA"
                     /locus_tag="VHS97_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX3448918.1"
                     /translation="MKKKKVRVALKKNRQNRTRDNDLTRKFRDDELTSAATPAVDRIR
                     PKGDLSRHRTIIEDVEEGRQPTASSMNPDASARRSVDTSTCLRGRVIRVHGLCSMVRS
                     DDGRTFSCQVRRLLKTLAIEGRSIVTVGDHVWFRPGSAPGSDGLIEKVEARRGLITRG
                     YRHRQHIIASNVDQVLIVSALAEPGLKLGLIDRYLVSAEMGDVAAVIVLNKADLVDLA
                     PWQWVIGLYTQLGYETVVTSVADGRGLGRLQQLLSSGVTAVTGQSGVGKSSLLNIVQP
                     GLNLKVREVSDWTFKGKHTTTTAELIGLESGGFVVDTPGLRQFELWGAVPGELGGYFI
                     EFRPFIPHCRFPDCSHTHETGCAVKDAVYWGQIHPGRYESYRKLYERQPLEGD"
     gene            complement(2777..3223)
                     /locus_tag="VHS97_11730"
     CDS             complement(2777..3223)
                     /locus_tag="VHS97_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3448919.1"
                     /translation="MQTVLRTYFPLSPFNGRTLSRVDSWFDRVFGEDGEGFRPATAAG
                     NFAVSVWGDEKNLHVEAELPGVLENDLDISVHDGVLSIRAERRAEEGRAYLYNGRSFG
                     HFERTIVLPEEVDTENVEATLSNGVLRIDLPKHPAARPRKIALKTS"
BASE COUNT          754 a         1140 c         1120 g          731 t
ORIGIN      
        1 cgattctgct cgaggacaag tatgtcgcgt tcggggtgtc gtcggactca gtgcgagcgg
       61 ccttgacggc cgtgcgccgc aaggactgga aaccctcttc cagtcttgag aaagcctgcg
      121 aacacgttcc gccaaacttg gtcatactca gcgtgaccga cgtgagcgag agtctttcgt
      181 cgatgctggc aagcttgccg ggcacgcttc agacgatgat caacacgtcg atcgcactgg
      241 ccaaagcccg tgccagcggc actggcacgg gcacgaatgc ggctccggga aacggccgcc
      301 aacctggagg acccgctgga atgcctgccg gcggtccgac gtcgggcggg agcggttcaa
      361 tgcgggctat gatgcgaccg ggtggccccg gcggggctag tagacccggt ttcggccccc
      421 aggcgaacag cggttcaagc tcacccggag gtacggcagg agacgccgca agtggcacca
      481 ccaccgaaaa catgattgtg ctcaaggtcg atcccgacaa gctccccagt gccgccgatc
      541 tcaaggccca tttgtttccg acgactctgt cagtcaccgt gagtgacccc gaaatccggg
      601 tcgtcacccg cggggctttt cccgaccttt cgttgcccct gggaattgtg tccgccggat
      661 cgatgatgcc atttctgaag ccgttaatcg acgggcaacc gcaggcccaa gcaggaggcg
      721 cttcagggac tcaggcggga gcaccggcca ccacgcccgc ggccggcgcc ccggcggccg
      781 gcgccggaac tctcaaagga cggatgcggg gagggcgcgg ccgcggtgga ccggagtagt
      841 cgacctctgt ttgtctctct ttcgttggaa accgcagctc ctctcgaagc ttgtcggctc
      901 aatcagacag acggtcgtcc tgctctcgtt gccggacgac ttcacgccgg catttgtgtt
      961 ttctcattga acggaggaca cttcgatgga attcgtgcct gaggtgaccg ccttcctcga
     1021 gaacaagccg gggcgactcg ccaagatatg ttcggctctc gcccaggaaa agatcgatat
     1081 tcgggcgctc tcggtgatgg agactgacgg acccagtgtc ttgaggttca tcaccagcga
     1141 gctggagcag accaagaacg tgctgacttc gctgggaacc gaattccgga tcgccgaggt
     1201 attggcggtc cagatggaaa accgcaccgg ctcactggcc agggtgctgg aacggcttgc
     1261 tgccgaaaat atcaatgtcg aatacgctta cgcatcgaca acgactgctc cgcgcaaggc
     1321 gctggggatc ttccacacat cgaaccccaa gcgcgccagt caaatactaa atgaagccgg
     1381 ggcggcgctt gccggtggag cgcggtcggc cggacggcgc ccgttgcata cgcgctaacg
     1441 tgttggcccg aaaatgggct cgtcgagcga tccgccggcg aatccgcccg cttcggaggc
     1501 gagtttcacc gtgaagaaaa agaaagtgcg ggtcgctctc aagaagaacc gccagaatcg
     1561 gacgcgggac aacgacctga cccgaaaatt ccgagatgac gagctcacgt ccgccgctac
     1621 accggccgtc gatcgcattc gtcccaaggg agacctgtcg cggcatcgca caattatcga
     1681 agacgtcgag gagggccggc agccgaccgc gtcatcgatg aatcccgacg cttccgcccg
     1741 gcggtccgtg gatacgtcaa cctgcttgcg cgggcgcgtg atcagggtac acgggctctg
     1801 ctcgatggtc cgcagcgacg acggccgaac cttttcttgc caggtccggc ggttgctcaa
     1861 gaccttggca atcgaggggc ggagcatcgt cacggtggga gaccatgtat ggttccgacc
     1921 gggctcagct cccggctccg acggcttgat cgagaaagtc gaggcacggc gcggtctgat
     1981 cacacgcgga tatcgtcatc ggcagcacat catcgcttcc aacgtcgatc aggtgttgat
     2041 cgtctccgcc cttgccgagc ctggtctcaa gctcggtctc atcgaccgtt atttggtgtc
     2101 ggccgaaatg ggagacgtgg ccgcagtgat cgttctcaac aaggccgacc tggtcgatct
     2161 cgccccgtgg cagtgggtga ttggacttta tacccaactt ggctacgaga cggtcgttac
     2221 gtcggtggcc gacggtcgcg gcctgggccg cctgcagcaa ctgctttcaa gcggggtcac
     2281 ggccgtgaca ggccagagcg gcgtcggtaa gagctcgctt ctgaacatcg tccagcccgg
     2341 gctaaacttg aaggtacgcg aggtatccga ctggaccttt aaaggcaagc ataccacgac
     2401 cacggccgag ttgatcggcc tggaaagcgg cgggttcgtt gtcgacacgc ctggccttcg
     2461 ccagttcgag ctctggggtg ctgttccggg cgaactgggg ggatacttta tcgagttccg
     2521 ccccttcatt cctcactgcc gtttccccga ttgctcgcac actcacgaaa ccggctgcgc
     2581 tgtcaaagac gccgtgtatt ggggccaaat tcatcccggt cgctacgaaa gctatcgcaa
     2641 gctctacgag cggcagccgc tcgaagggga ctaatcagaa agccggcggc ccgaaggagg
     2701 gccaaccggc tcgaatgcat caaaccaccg tcggtaacgt cgtttgctgc cgtcggcgcg
     2761 atatcgaacg atacgatcac gatgtcttga gcgcgatctt tctggggcgt gctgcggggt
     2821 gcttgggcaa gtcaatccgc aaaacgccgt tgctcaaggt cgcttccaca ttttcggtgt
     2881 cgacttcctc ggggagaacg atcgttcgct cgaagtggcc gaaagaacgg ccgttgtaaa
     2941 gataggcccg gccttcctcc gcccggcgtt ccgctcggat actcaagacc ccgtcatgga
     3001 cgctgatgtc caaatcgttt tccaacacgc ccggcaactc ggcctcgacg tggagatttt
     3061 tctcatcccc ccacacggaa accgcaaagt tgcccgccgc cgtcgctggg cgaaagccct
     3121 cgccatcctc gccaaaaaca cggtcgaacc atgaatccac gcggctgagc gtccggccat
     3181 tgaatggcga tagcggaaaa taggttcgca gaaccgtttg catcttatgc gctccttaac
     3241 tgaatacttt gcttaaaaac agctagttct tggttgtctg tcgactcggt aacccccggt
     3301 tagccaagcc gtttgccgtc gatattgtag ccaagcaagc ggtatgccaa tacggcctaa
     3361 ttctataaac tgagttttac taacagctta gatatccccc tagtgttttc acgtgtccgt
     3421 gtgactgcca ctatctacag ctggaacttc ggaatgccat tttggcatac acttttgttc
     3481 gggctaataa gacggctgcc atttcgtact tctcgagagc tggtcaatcg agccaccgac
     3541 tgtcgaccta gtccggactt gaggcgcggc gcgaaccacc ccggcccgac gacacactaa
     3601 ctagaagtcg cacagtgcgc cgctcggaat aaacttggac gatttgtcga attccggttc
     3661 ggccattcga ctatttggcg aaggcagggc aaccggcccg cccgaagacc gttagcgggc
     3721 gccgggggca gtacgttcgc caacg
//
LOCUS       DATAQL010000670         7784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4953844, whole genome shotgun sequence.
ACCESSION   DATAQL010000670 DATAQL010000000
VERSION     DATAQL010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4953844"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..2059
                     /locus_tag="VHS97_11735"
     CDS             119..2059
                     /locus_tag="VHS97_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX3448920.1"
                     /translation="MDGTHANEAGLTVPRSRVPTFVLAASFLAFLLGAVWTFWHARQA
                     PPQPAAGLERHAGFWPESPWKNARPGVKYVGDASCIRCHEDIAETFRHHPMGRSLTPI
                     SSAPAVGVMQADGSSTFDVGSSRFTIDRRDGREAHRETRLDDNGRVLAQVEAEVKYAL
                     GSGTRGITYLVEHDGRLFQSPISWYRRKNQWDLSPGYEKSNLHFNRPIEPHCLYCHSN
                     RALSVELSVNRYKEPIFLGHAIGCERCHGPGELHARGQDVVDGRDLTIVNPKHLSPSL
                     RGAVCEQCHIVGDQRVDRLGREPFDYRPGLPLIAFQAVYGGVNGPGNKVGGHVEQMKN
                     SRCFRESQGRLGCTSCHDPHQIPGSQERIAYFRERCLACHDRNGCKLSRSVRIAESPD
                     DSCIQCHMQNSNSADVAHVATTDHRILRSTPRSVNEPDRGANGLPLVLLNGDNLDRNE
                     LESLGRELAIALASEGPLLPNTPRTKAMVPLVLSHLDAALAKHPDDLVALRMKAQVMA
                     LSGRRRDAIPLVEAVLKAAPYYEQALDQYVSYSIDEGDTQAALEPARRAVAVNPGSSV
                     FRERLAYVSMQHQHWDEALHESRDALRLDPFLRFARMFVIECLLHQKDLKHAEEELAT
                     LIKLNPSQRESLTQWFAEQRRN"
     gene            2172..4112
                     /locus_tag="VHS97_11740"
     CDS             2172..4112
                     /locus_tag="VHS97_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448921.1"
                     /translation="MATRDADETRQPRSRSRVPILVLTALILLLGLGFGWTLWQARHA
                     TGTPALSSSRQSRSSLETPWKNARPGVKYMGDSVCIRCHGDIAETFRRHPMGRSLAPI
                     TSAPPVGTDRSEGSITLDVGSSRFTIERRGGREVHRETQRDEAGRVLAQVEGEVKYAL
                     GSGTRAISFLVEHDGRLFQSPITWYSQKKQWDLSPGYSEHSRHFDRPIEQSCLYCHSG
                     RVEPVDLSVNRYKEPIFQGHAIGCERCHGPGELHSQRQEEIDGRDVTIVNPRHLEPAL
                     RAAVCEQCHLLGDHKVDRLGRGPFDYRPGLPLIDFYAVYGRVNEAANHAVGQVEQMKL
                     SRCFRATRGRLGCTSCHDPHQVPPLAEKTAYFRQQCLACHERKGCSLPESVRLAESTN
                     DNCIQCHMKQLTKVDIAHVAITDHRILKTPDARTPRPDRATHGLPLVLLNGDELSPVE
                     LGSLGRELAIALTLEGPSLPESPNVRRMGSLVLALLNEALAKQPDDLFARLMKAQALV
                     LSGRRGDAFSLVEAVVRASPSYEMALDQFLVLAIDEGHTQAALEPARRAVAVNPWSSA
                     FHERLAYVSIQHQHWDEALHESREALRINPFLRVARTFVIECLLRQKDTKRAEDEFAT
                     LLKLNPSQRESLTQWFAEQRRN"
     gene            complement(4113..7010)
                     /locus_tag="VHS97_11745"
     CDS             complement(4113..7010)
                     /locus_tag="VHS97_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX3448922.1"
                     /translation="MTRFRLLIVFLGLGGLLAGWMAVRAVEKRGLESELREARGEFGA
                     RRFGAARARLARLAQRWPDHGEIQYLLGHCEKIRGHLDAAMAAWDHVPAAANLAPLAD
                     LSRGRLALEIGRYRLAETCLERARLAAGEVDDEACRLLEWLYWMTGRRDEHRELLRQR
                     VERTDDPSQTLRTLWGSDRDPYPVDGIAVALAKANQSAPDDDLVWLGLADVAIRTGRF
                     DEAGDWLSRCEQARPDDLAVWSARLEWARAAGRPAEVVRAANHLPASRVSQPRIVAIR
                     AWLAARNGDSRAERSALDELVALQPGDVAALERLADLAAQAGEPERVAELRRRKAAVD
                     TAFERYRELINLPEMAPHAAEFARAAEPIGRWYDARAWWRFAVQRDSSVADEAAAALA
                     RLAKAEPAFEPDRRTLAELLGPIRPPGAPTATGLHGPLIPTFVDEAARRGLAFTFENG
                     WTDLRQLPETMSGGVAVVDFDGDGWLDVYAVQGGPFPPRKDNRPFGDRLFRNRGDGHF
                     DDVTAASGLAGLAGGYGHGVAVGDYDNDGRPDLFITRFGSYALYHNLGQGRFEDATAK
                     ADLGGDRDWPTSAAWADLDNDGDLDLYVCHYLKWDAQNPTLCEYPEHSKPGYLYCGPP
                     AFPALRDHVFRNDGGRFVDVTEAAGIVDRDGRGLGVVAADLDGDGKCDLFVANDTTAN
                     YFFRNLGGFRFSEQGMESGLASSAEGGYLAGMGVACSDFDGDGRLDLAVTNFFNQSTT
                     LYHSHGGGVFSDRSASTGLAAPTRQVLGFGLAALDANNDGWIDLAQANGHVTDFSPSI
                     PLEMKAQLFLADGAGRFVDISDRAGPPWQVLRIARGLAVGDLDNDGRTDVLIVSQNAP
                     LAMLHNQASSPNHWLTLSLEGTNSNRDAVGARVVVTADGKTQAAARIGGGSYLSASDP
                     RLHIGLGSAKKADRIEITWPSGRRDSYQGLAADTGYRLREGDPSARPLAGF"
     gene            complement(7122..>7784)
                     /locus_tag="VHS97_11750"
     CDS             complement(7122..>7784)
                     /locus_tag="VHS97_11750"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3448923.1"
                     /translation="TTTARYSWPPNTGYACCTPDPFNFTGKGSPAQKPASTGMFAYWV
                     SYGIQDATDGSSNTIIFAESLVGDSGGTQASSASNRNNSALGVTAAGTAEAYDASTLN
                     YQTVILPAVQACAAAKLTGGTSLATDNGNRWAWGAVGMTLFNTVIPPNGAPFSDCRDD
                     CPACSPDDSLFSNVQSYHPGGANVLLADGSARFIKNSISPQTWMGIGTKASGEVISAD
                     SY"
BASE COUNT         1464 a         2475 c         2457 g         1388 t
ORIGIN      
        1 cctacacaaa tagttcggcg gtctcactta gcgtgcgagg ggagaaggac cactctgccg
       61 cggccgtttg gagtcgcctt tatctgcggc cgcagtcgct gcggggaggg aggatctgtt
      121 ggacgggacg cacgcgaatg aagccggact aacagtacct cgctcccggg tcccaacctt
      181 cgtgttggcg gcatcgttcc tggcgttctt gctaggggcc gtctggacgt tctggcacgc
      241 gcgtcaagca cccccgcagc cggcggcggg tctagaacga cacgcaggtt tttggccaga
      301 gagcccgtgg aaaaatgcac gaccgggcgt gaaatacgtg ggcgacgcgt cctgcatccg
      361 ctgccacgaa gacatcgccg aaacgtttcg acaccatccc atgggacggt ctctgacccc
      421 gatctcctcc gcgcctgccg tcggcgtcat gcaggccgac ggttcgtcca cattcgatgt
      481 cggctcatcc cgatttacaa tcgatcgtcg cgacggccgg gaagcgcatc gcgagacccg
      541 gctcgacgac aacggtcgag tcttggcaca agtagaggcg gaggtcaaat acgcgctggg
      601 gtcgggcaca cgcggaatca cgtatctggt cgagcacgac ggccggttgt ttcagtcgcc
      661 aatctcatgg taccgtcgga agaatcagtg ggacctgtcg cctggctacg agaagagtaa
      721 cctgcacttc aatcggccga tcgagccgca ttgcctttat tgtcattcca atcgcgccct
      781 atcggtcgag ctgtcggtca atcggtacaa agagcctatt tttctcggtc acgcgatcgg
      841 ctgcgagcgg tgccacggac cgggcgagct ccacgcccgt gggcaggatg tggtcgacgg
      901 ccgggatttg acaatcgtca atccaaagca tttaagtccg tcactgcgcg gggcagtttg
      961 cgagcagtgt cacatcgtgg gcgatcagag ggtcgaccgc ctgggccgtg agccgttcga
     1021 ctatcgtccc gggcttccct tgatcgcgtt ccaagcggtc tacggcggcg tgaacgggcc
     1081 tggaaacaaa gtcggcggac acgtcgagca gatgaaaaac agccggtgct tccgcgagag
     1141 ccagggtcga ctgggttgta cttcgtgcca tgacccgcat caaattcccg gatcgcaaga
     1201 acgaattgcc tatttccgcg agcggtgcct ggcctgccac gaccgcaacg gatgcaaact
     1261 ttcccgctcg gtacgcattg ccgagagccc cgacgacagt tgtattcaat gtcatatgca
     1321 aaattccaac agtgccgatg ttgcgcacgt cgcaacgacc gaccaccgga tcttgaggtc
     1381 aacccccaga tcggtgaatg agccagatcg gggcgctaat ggacttccgc tcgtcctgct
     1441 caatggcgac aatctggatc ggaacgaact cgagtccctg gggcgggaac tggcaatcgc
     1501 cttggcgtcc gaagggccac tgctgcccaa tacaccgcgg accaaggcga tggtacccct
     1561 cgtcctgtcg cacctcgatg cggcacttgc aaagcatccc gatgatctgg tcgccctgcg
     1621 catgaaagct caggtgatgg cgctctcggg acgccgccgg gatgcaatac cgctcgtcga
     1681 agcggtcttg aaagcggccc cttattacga gcaagccctc gaccagtacg tctcgtattc
     1741 gatcgacgag ggcgacaccc aggctgccct ggaacccgcc aggcgggccg tcgccgtcaa
     1801 tcccgggtca tccgtgtttc gcgagcggtt ggcctacgtt tcaatgcagc atcaacactg
     1861 ggacgaggcg ctccatgagt cgcgcgacgc acttcggctc gatccattcc ttcgcttcgc
     1921 gaggatgttc gtgatcgaat gcctccttca ccagaaggac ctcaagcacg ccgaggagga
     1981 gctcgcgacc ttgatcaagc tcaacccgag ccaacgagaa tccctcacac agtggtttgc
     2041 cgaacagcgg cggaattagt tgatcaagtt atttctgcga ggctcctcag tcaaagaacc
     2101 atgcttcttg gccgctacgc gagctctggc tcaatttcag aggggcagtc ctcctccgga
     2161 gggatgatcg gatggcaacc agggatgcgg atgaaacccg gcagccgaga tcgcgctccc
     2221 gcgtaccgat cttggtgctg acggcgttga tcctgctttt gggactcggc ttcggctgga
     2281 cgctgtggca ggctcgtcac gcgaccggga cgccggcgtt aagttccagc cggcagtcgc
     2341 ggtcgtcgct cgagacgccg tggaagaacg ctcgacccgg cgtgaagtac atgggcgact
     2401 cggtctgtat tcgatgccac ggggacatcg ccgagacgtt tcgccggcat cccatggggc
     2461 ggtcgctggc cccgatcacc tcagcgccgc cggtcgggac cgatcggtcg gaaggttcga
     2521 tcaccctcga cgtcggatcc tctcgcttca ccatcgaaag gcgcggcggc cgcgaggttc
     2581 accgcgaaac ccagcgcgat gaggccggtc gagtcctggc gcaagttgag ggagaggtga
     2641 agtatgccct gggctcgggc acacgcgcaa tttcgtttct ggtcgagcac gacggccggt
     2701 tgtttcaatc gccgatcacg tggtacagcc agaagaaaca gtgggacctg tctcctgggt
     2761 actcggaaca cagccgccac ttcgatcggc ccatcgagca gagttgcctg tattgtcatt
     2821 ccggccgcgt agagccggtt gacctgtcgg tcaaccgcta taaggagccg attttccagg
     2881 gccacgcgat cggctgcgag cgatgccacg ggcctggtga actgcactca cagcgccagg
     2941 aggagatcga cggccgagac gtgaccattg tcaatcctcg gcacttagag ccggcactcc
     3001 gtgcggccgt ctgcgagcag tgtcacttgc ttggcgatca taaggtcgat cggctgggcc
     3061 gagggccgtt cgactatcgt cccgggctgc cattgatcga cttctacgcg gtttacggcc
     3121 gggtgaacga ggcagcgaat catgccgtgg gacaggtcga gcaaatgaaa ctcagccggt
     3181 gtttccgcgc gactcgcggc cgtctcggct gcacctcgtg tcatgacccc caccaggttc
     3241 cgccgctcgc cgagaagacc gcctacttcc gccaacagtg tctggcctgc cacgaacgaa
     3301 aggggtgtag ccttcccgag tcagtacgcc tggccgagag cactaatgat aattgcatcc
     3361 agtgtcacat gaaacaatta acaaaagttg atattgcgca cgtcgcaatc acggatcacc
     3421 gcattctgaa aacacctgac gcacggacgc caagaccgga ccgggcgacc catgggctac
     3481 cgctggtcct cctaaacggt gatgagttga gcccagttga actcggctcg ctggggcgtg
     3541 aactggcgat cgctctgacg ctcgaggggc caagtctgcc cgagtcgccc aatgtcaggc
     3601 gaatgggatc cctcgtgctg gcccttctga atgaggcgtt ggcgaaacag cctgacgacc
     3661 tgttcgcccg actcatgaaa gcgcaggcgc tcgtgctgtc agggcgacgc ggagatgcgt
     3721 tttcgttggt cgaagccgtg gtgagagcgt ccccttcata cgaaatggcc ctggatcagt
     3781 tcctggtgct cgcgatcgac gaaggacaca ctcaggctgc actggaaccc gccagacggg
     3841 ccgtcgccgt caatccctgg tcatcggcat tccatgagcg gttggcgtac gtttcgatac
     3901 agcatcaaca ctgggacgag gcgctgcatg agtcccgcga ggcgctccgc atcaatccat
     3961 tcctgcgcgt cgcgcggacg ttcgtgatcg aatgcctgct ccgccagaaa gatacaaagc
     4021 gtgccgagga cgagttcgcg accctactga agctgaaccc gagccagcga gaatccctga
     4081 cacaatggtt cgccgaacaa cggcggaatt agtcaaaaac cagccagcgg ccgggcggac
     4141 ggatcaccct cacgcaggcg gtagccggtg tccgcggcaa ggccttgata cgagtcacgc
     4201 cggcccgaag gccatgtgat ctcgatacga tccgccttct tggccgagcc cagcccgatg
     4261 tgaaggcggg ggtcgcttgc cgagagatag cttcctcctc cgatccgggc ggccgcttgc
     4321 gttttgccgt cggcggtcac gaccacgcga gcgccgacgg cgtcgcgatt cgagttggtc
     4381 ccctcgagcg agagcgtcag ccaatggttt ggggacgatg cctgattgtg cagcatggcg
     4441 agcggggcgt tctgagagac aatcaaaacg tcggtgcggc cgtcattatc gaggtcgccg
     4501 acagccagtc cgcgtgcaat ccggaggact tgccagggtg gtccggcgcg gtccgagata
     4561 tcgacgaacc gccccgcccc gtcggcgagg aagagctgcg ctttcatttc cagcgggatc
     4621 gacgggctga aatcggtaac atggccgttg gcctgcgcca ggtcgatcca gccgtcattg
     4681 ttggcgtcca aagcggcaag cccaaagccg agcacctgtc gggtgggcgc cgcgagtccc
     4741 gtggaagcgg accggtcgct gaaaacccca ccgccgtgac tatgatacag cgtggttgac
     4801 tggttgaaaa aattggtcac ggccaggtcg agccggccgt ccccgtcgaa gtcgctgcat
     4861 gccacgccca ttcctgccag atagcctccc tcggcgctcg acgctagtcc cgactccatc
     4921 ccctgctcag agaagcggaa gccgccaagg ttccggaaga agtaattggc cgtcgtatcg
     4981 ttggcgacga acaggtcgca cttgccgtca ccatcgagat cggccgcaac gacacccaag
     5041 ccgcgaccgt cgcggtcgac gatgcccgcc gcctcggtca cgtccacgaa ccggccgccg
     5101 tcgttgcgga agacatggtc ccgcagagcg gggaaggcgg gcgggccgca gtagaggtat
     5161 cccggtttgg aatgctccgg atactcgcaa agggtcggat tctgggcatc ccatttcaaa
     5221 tagtgacaga catagaggtc caggtcgccg tcgttgtcga ggtcggccca ggccgccgag
     5281 gtcggccagt cgcggtcgcc gcccaagtcg gccttggcag tggcgtcttc gaaccgccct
     5341 tggccgaggt tgtgatagag ggcgtatgac ccaaatcgag tgataaagag gtccggccgg
     5401 ccgtcgttgt cgtagtcgcc aacggccacg ccatgtccat agccgccggc aagccccgcc
     5461 agtcccgatg cggcggtcac gtcgtcaaag tggccgtcgc cgcggttgcg gaaaaggcgg
     5521 tcaccgaacg gccggttgtc tttgcgcggg ggaaacggcc ctccttgaac ggcatagacg
     5581 tcgagccatc catcgccgtc aaaatccacc acggcgacgc cgccgctcat cgtctcgggg
     5641 agttggcgaa ggtcggtcca accgttctcg aaagtgaagg ccaggccacg ccgcgcggcc
     5701 tcgtccacga acgtgggaat caggggaccg tggaggccgg tggctgtcgg cgcccctggc
     5761 ggccggatcg gaccgagaag ttcggccagg gttcgtcggt cgggctcgaa tgccggttcg
     5821 gccttggcca gccgagcgag ggcggcggcg gcctcatccg cgaccgacga atcccgctgg
     5881 accgcgaaac gccaccatgc ccgggcgtcg taccaccggc cgatcggctc ggcagcacgg
     5941 gcgaactcgg ctgcgtgcgg cgccatctcg ggcaaattga tcagctcgcg gtagcgctcg
     6001 aatgccgtat cgaccgccgc tttgcggcgc ctcagctcgg cgacgcgttc cggctcgccg
     6061 gcctgggcgg cgaggtcggc cagccgctcg agtgcggcga catctccggg ctggagggcg
     6121 accagttcat cgagggccga ccgctcagcc cggctgtcgc cattccgcgc cgcgagccag
     6181 gccctgatcg cgacgatcct gggctgggag actcgcgaag ccggcagatg gtttgcggct
     6241 cggacgactt cagccggccg gccggcggcc ctggcccatt ccaggcgagc ggaccacacg
     6301 gcgaggtcgt caggccgcgc ctgctcgcat cgactcagcc agtcgccagc ctcatcgaaa
     6361 cgccccgtgc ggatggccac gtcggccagc cccagccaga caagatcgtc gtccggagcg
     6421 gactgattgg ctttagccag tgcgacggcg ataccatcga ccggatacgg atccctgtcg
     6481 gagccccaga gggtgcgcag cgtctgcgag ggatcgtccg tccgctcgac tcgttgacgc
     6541 aatagctccc gatgttcatc gcgacggccc gtcatccagt agagccattc gaggagccga
     6601 cacgcctcgt cgtcgacctc gcccgccgcc agtcgggcgc gctcgaggca cgtctcagcg
     6661 agccggtagc gaccgatttc caaggccaga cgccctcgtg acaagtccgc cagaggagcc
     6721 aggttcgctg cagcggggac gtggtcccac gccgccatcg cggcgtccag gtgtccccgg
     6781 atcttctcac aatggccgag caagtactga atttcgccat ggtcgggcca tcgctgggcc
     6841 agccgggcca gccgcgcgcg ggctgcgccg aatcgcctcg cgccaaactc gcctcgcgcc
     6901 tcgcgcagtt cggactcaag cccgcgcttc tcgacagcgc ggaccgccat ccatccggcc
     6961 agcaggcccc ccaaccccag aaatacgatc agcagacgaa agcgcgtcaa ggtccagtca
     7021 gcccatacga aacagcaaga tccggcgggg tcagagacac cgccggacct tgtctatgct
     7081 taccgcaacg atcggacgcg ggccgcccga ttccgaatcg gtcaatacga gtcggcgctg
     7141 atgacttcgc cactggcttt tgtgccaatg cccatccagg tctgcggact gatcgagttt
     7201 ttgataaacc gtgcgctgcc gtcggccagc agaacgttgg caccgccagg atggtagctc
     7261 tggacattgg agaacagcga gtcgtccggg ctgcacgccg ggcaatcatc gcggcagtcg
     7321 ctaaacgggg caccattcgg cggtatcacc gtgttgaaca gcgtcattcc cacggcgccc
     7381 caagcccagc ggttgccatt gtccgtggcg agactcgtgc cgcccgtcaa tttggccgca
     7441 gcgcacgctt ggacggcggg gaggatgaca gtctggtaat tgagcgtact ggcgtcatat
     7501 gcttcggcgg tcccggcggc agtgacgccc agagccgaat tgttccgatt cgaagcacta
     7561 ctcgcctggg tgcctccgga gtcccccacc agtgattcgg cgaagatgat cgtgttcgac
     7621 gatccatcgg tggcgtcctg gatgccatag ctgacccagt aagcgaacat tccggtcgag
     7681 gcaggctttt gcgccggtga acccttgccc gtgaaattga acgggtcagg tgtgcagcaa
     7741 gcatatccgg tgttcggagg ccagctgtac cttgcggtag tcgt
//
LOCUS       DATAQL010000671         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_69093, whole genome shotgun sequence.
ACCESSION   DATAQL010000671 DATAQL010000000
VERSION     DATAQL010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69093"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VHS97_11755"
     CDS             <1..968
                     /locus_tag="VHS97_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448924.1"
                     /translation="TRLVRSLLGQHRFPEALEAAGSAAALDPKAWLLVADVELELGRG
                     DKARSAFAQAAVDPEDLSAIVLRARFADADGDPDRALQLLREAARRADELSDLPSEAA
                     AWFHVMVGHTLIDRGRLDEGTTACRTALAIFPRDYRAMTGLAEAAAFRCEWDQVIAWA
                     SRAIEAAPQNPEALKLLVEAYAASGDHKAAERQHRRFEELAHSFPRIYDRHWALYSAD
                     HDQNLDEAYALATRDLELRTDAAAYETLAWVAFKKGLQSEAESAIRSALARKPQTASI
                     FHRAAIITQAGGDRRGAESFLGRARDLNPYLVKMTNPAKPRSATN"
     gene            982..1782
                     /locus_tag="VHS97_11760"
     CDS             982..1782
                     /locus_tag="VHS97_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX3448925.1"
                     /translation="MKLPRLRTGFTLIELLVVMAIISVLAGLLLPGVQQAREAARRID
                     CQSHLHQIGLGIMQYFDDWNGQFFLHHPFNADSLSEVGNAESFAEIYWEDKIMPYVNP
                     TFANDQLAKSGVQIADEKIFRCMSDTSIVTPYISPTTGLVDGISNRTSYLLNSQLSHK
                     TVRYGRWTLPRFQYDIGTSNFVCMNERDGAVMDANIRDPVAATDPRQDDYDTWLGTVT
                     LDKWIPWNRHGCSNVLYLDGHAKSVLKPDAYLGMYPGGVILTDLSWYP"
     gene            complement(1986..3419)
                     /gene="cpaB"
                     /locus_tag="VHS97_11765"
     CDS             complement(1986..3419)
                     /gene="cpaB"
                     /locus_tag="VHS97_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX3448926.1"
                     /translation="MGCATISKSAAVIVLVVFLGLGVVELISHIEAAESARPMSQSEP
                     EARSQVSPPEPATLVRSYETSLAPYSRMRGRWTLRLLKDEPVVTGKLAPKGSGRGMAA
                     LIPKGMRAFTIQTPSIASGGAGFVMPGHKVDVLLTMNDVGTNDQTVGGSTTTLVQNVE
                     IRAVDQNIDAPAENKMDKDLRSVTLLVTPQQAKHLDLGQNKGTLHLAFRNESEEWAVF
                     RDHDRTRVIKTTNSEKGVVVFESIRQGKLLVSVFADGKVLGWLQPSAQQDSNQLGGSL
                     CSPCYGIVDQEWIPNFLRTANTSVEAETLEGHPLFRLRGLTMDAKIDVWIDPSLNYAA
                     RRIRFDKRASEADPGIRSGQFDATRFRLEKGHYVVTEATTTWTVGPQPIVSPLVVEKM
                     VKGKRVKTYLPATDMNGNILMDQRRYTEKIMLRDIDFDPRWTDRDFQFSRPMTNGTRA
                     EIQGAPDPNYVWKDGWVVLTAHDPTPK"
BASE COUNT          641 a         1117 c         1035 g          728 t
ORIGIN      
        1 tgacgcgcct ggtccgaagt ctgctcggcc agcacaggtt tcccgaggcg ctcgaggccg
       61 ccggcagcgc cgcggcgctc gaccccaagg cctggctgct cgtcgctgac gtcgagctgg
      121 aactgggtcg tggcgacaaa gcccgatccg ccttcgccca ggccgctgtt gacccggagg
      181 acttgagcgc gatcgtctta cgcgcccgct tcgctgatgc cgacggcgac cctgaccgcg
      241 cccttcaact gcttcgggag gcggctcggc gagccgacga gttgagcgat ctgccgtcgg
      301 aggccgcggc ttggttccac gtgatggtcg ggcacactct gatcgaccgg ggacgcctcg
      361 acgaaggcac caccgcctgt cgaacggcac tggccatctt tccccgcgac tatcgggcga
      421 tgactggcct ggcggaagca gcggcattcc gatgcgagtg ggaccaagtc atcgcctggg
      481 cgtcgcgcgc aatcgaagcc gcgccacaaa accccgaggc tctgaagctc cttgtcgagg
      541 cgtatgccgc gagtggcgac cacaaggctg ccgaacgcca gcaccggcga ttcgaggagt
      601 tggcccactc attcccccgt atctacgacc ggcactgggc cctctacagc gccgaccacg
      661 accaaaacct tgacgaggcc tatgctctgg ccacgagaga cctcgagttg cgcaccgacg
      721 cggccgccta tgagaccctg gcttgggtgg cattcaaaaa ggggctgcag tctgaagccg
      781 agtcggcgat ccgctcggcg ctggcgcgca agccgcaaac cgcctccatc ttccaccgcg
      841 ccgcgatcat aactcaggcc ggaggcgacc gccggggcgc cgagtcgttc ctgggccggg
      901 cccgcgactt aaacccctat ctcgtcaaaa tgaccaaccc agccaaaccg agatctgcga
      961 ccaattgatt ggagtcattg aatgaagctt ccacgattac ggacaggttt cacgctgatc
     1021 gagctcttgg tcgtgatggc gatcatttcg gttttggccg gactgctgct tcccggtgtg
     1081 caacaagcgc gtgaggcggc ccggcgtatc gactgccaga gtcatctgca ccagatcggc
     1141 ctgggaatca tgcaatactt cgacgattgg aacgggcagt tctttctgca tcacccgttc
     1201 aacgccgaca gtctgtctga ggtgggcaac gccgagtcgt tcgccgagat ctactgggaa
     1261 gacaagatca tgccctatgt caatcccacg ttcgccaacg atcagctcgc caagtcggga
     1321 gttcagatcg ccgacgaaaa gatcttccgc tgcatgtccg acacgtcgat cgtcacgccc
     1381 tatatcagtc ccaccaccgg gcttgtcgac gggatctcca acaggacgag ttacctgctc
     1441 aattcgcaat tgagtcacaa gaccgttcgc tacggccgct ggacgttgcc gcgcttccag
     1501 tatgacattg gcacgtcaaa ttttgtctgc atgaacgagc gggacggcgc ggtcatggac
     1561 gccaatatcc gcgaccccgt cgcggcgacc gacccgaggc aagacgacta tgacacctgg
     1621 cttggcacgg tgacgctcga caaatggatt ccttggaacc ggcacgggtg ctcaaacgtg
     1681 ctttacctgg acggtcacgc caagtcggtg ctcaagcccg acgcctatct gggcatgtac
     1741 cctgggggcg tgatactcac tgacctcagt tggtatccat aacatttcta catatttcat
     1801 actacggaat tacaggcaac tccgttgctc tgtcccgata ccgatggttt gtgaccggca
     1861 tctggaggaa atggctctcg cggcgtcgtt cttgcaatgc gactgcgccc tcgaccgagt
     1921 ttgcccgaga cctcgtctgc gtcgaccgac cccacaccgc ggttcgtgag gatcggtttc
     1981 ggcggtcatt tcggagttgg gtcgtgggca gtcaagacga cccatccgtc cttccatacg
     2041 taattgggat caggggcgcc ctggatctcg gctctggtgc cgttggtcat cggccgtgag
     2101 aactggaaat cacgatcagt ccatctcgga tcgaagtcga tgtcgcgcag catgatcttc
     2161 tcggtgtacc ggcgctggtc catgaggatg tttccgttca tgtccgtggc cggcagatag
     2221 gtcttcactc gcttgccttt caccatcttt tcgacgacga gcggtgagac gatcggctga
     2281 ggtccaacgg tccatgtcgt cgtggcttcc gtcaccacgt agtggccttt ttccagccgg
     2341 aatcgcgtgg cgtcgaactg gcctgagcgt atgcccgggt cggcttcgga ggctcgttta
     2401 tcgaatcgaa tcctccgcgc ggcgtagttg agcgaggggt cgatccagac atcgattttg
     2461 gcgtccattg tgagccctcg tagccggaag agagggtgtc cttcgagtgt ctccgcctcc
     2521 actgatgtat ttgccgtccg caagaagttc ggaatccact cctgatctac gatcccgtag
     2581 cagggcgaac agagcgaccc gcccaactgg ttcgagtctt gctgtgccga cggctggagc
     2641 cagccaagga ctttcccatc ggcaaagacg ctgaccagta gcttcccctg ccgtatggac
     2701 tcgaagacca ctacgccttt ctcgctgttc gtcgtcttga tcaccctcgt gcgatcgtgg
     2761 tcgcggaaga cggcccactc ttcactttcg ttccggaacg ccaggtgtag cgtccccttg
     2821 ttttgcccca ggtcgagatg ttttgcctgc tggggcgtga ccagcagagt caccgaccgt
     2881 aggtctttgt ccatcttgtt ttcggcgggg gcatcgatgt tctggtcgac ggcccggatc
     2941 tcgacgttct ggaccaaggt ggtcgtgctg ccaccgacgg tttgatcgtt tgtgcccacg
     3001 tcgttcatgg tcaagagcac atccaccttg tggccgggca tgacaaaccc ggccccgccc
     3061 gaggcgatgc tgggcgtttg gatcgtgaag gcccgcatcc ccttggggat cagagccgcc
     3121 atgccgcgcc ccgaaccctt gggggccagc ttgccggtta cgaccggctc gtctttcaac
     3181 aagcggaggg tccatcgtcc tctcattcga ctgtacggcg ccagactcgt ctcataactc
     3241 cgcacgagcg tggccggctc gggcgggctg acctgggacc gtgcctccgg ctcagactga
     3301 ctcattggcc tcgctgattc ggctgcctca atgtgggaga tcagctcgac cacccccaga
     3361 ccaagaaaga cgacaagcac aatcacggcc gcggacttgc tgatcgtagc gcatcccatg
     3421 gatttcagaa ctccttcggt gaggtcatgg ccatgccgat gactgccacc agtatgatcc
     3481 agcgccgggc cgtcatccag ggcactcgta tgactccctt g
//
LOCUS       DATAQL010000672         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1443364, whole genome shotgun sequence.
ACCESSION   DATAQL010000672 DATAQL010000000
VERSION     DATAQL010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443364"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2092
                     /locus_tag="VHS97_11770"
     CDS             <1..>2092
                     /locus_tag="VHS97_11770"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX3448927.1"
                     /translation="TVKTDADGQVQVPAVNPNDPGGQYQWLITATTTEGRFAQLGFIN
                     IWSFGQLNTDFDQVKVYAITDRPVYRPASPVRFKFWVAHSRYGETAGSDFAGKAFPVE
                     IHSPKGDKIFAKTYESDAFGGFDGSFELSSDATLGVYHVFIPNRGGGTFRVEEYKKPE
                     FELKVDAPPRPVMLGEKVTATIKASYYFGGPVTEAKVKYKVTRTIADERWYPVARWDW
                     LFGSGYWWFAGDYSWYPGWSSWGMRRPAFAWWGHPQAPPEVVADAELPIGPDGTLTVE
                     IDTAAAKAAHPDEDHRYAITAEITDQSRRTIVGSGTVLVARKPFAVYTWVDRGHYRAG
                     DTIEAQVHAQTLDHKPVAGKGTIKLLKIDYGQERRPVETQVESWELSLDPAGVARQTL
                     KAAAAGQYRLAATIEDGKGHAIEGGYVFTITGQGFDGASYRFNDLEVIPDRKEYRPGE
                     TVRLLINTNRVNSTVLLFVRPTNGVYLEPKVVRLQGKSTVEEIGIAPRDMPNIFVEAL
                     TVADGKVHDEVREIAVPPESRIVDVAIEPAQTSYKPGQRAKVKVKLTGPDGKPFEGSS
                     VLTVYDKAVEYISGGSNVPDIKEFFWKWKRYHQPQTESSLDRWFSNLTKPNEVGMEDL
                     GGTDGLEPQFQPGAVAYRKRRSAMPMRGMAGMGGNRGVEFDLAMPAAVMAAPMAKADA
                     AVGGERQRHDVISAD"
BASE COUNT          432 a          628 c          670 g          362 t
ORIGIN      
        1 cacggtcaag accgacgcgg acggacaggt gcaggtcccg gccgtcaatc ccaacgatcc
       61 gggcggccag taccaatggc tcatcaccgc gacgacgacg gaaggccggt tcgctcagct
      121 gggctttatc aatatctggt cgttcggcca gctcaacacg gattttgacc aggtcaaggt
      181 ctatgcgatc accgaccggc ccgtctatcg gcccgcctcg ccggtacggt tcaaattctg
      241 ggtcgcccac agccgttacg gcgagaccgc aggatcggat ttcgcaggaa aggccttccc
      301 ggtcgagatt cacagcccca agggagacaa gatcttcgcc aagacctacg agtccgatgc
      361 atttggcgga ttcgacggct cgttcgagct ctcctccgac gcgacgctcg gcgtctatca
      421 cgttttcatt ccgaatcgcg gcggcggtac tttccgggtc gaggagtaca agaagcccga
      481 gtttgagttg aaagtcgatg ccccgcctcg accggtgatg ctgggtgaaa aagtgacggc
      541 cacgatcaag gccagctact atttcggcgg gcccgtcacc gaggccaagg tcaagtacaa
      601 ggttacccgc accatcgccg acgaacgatg gtatccggtg gcccgctggg attggctctt
      661 tggttcgggc tactggtggt ttgccggcga ctactcatgg tatccgggct ggtcgagctg
      721 ggggatgcgc cggcctgcgt ttgcgtggtg gggacatccg caggcaccgc cggaagtggt
      781 tgcggatgcc gagctgccga tcgggcccga cggaacactt accgtcgaga tcgacaccgc
      841 cgccgccaag gccgcccatc ccgatgagga ccatcgttac gcgatcaccg ccgagatcac
      901 cgaccagtcg cggcgcacga tcgttggtag cgggaccgtg ctggtcgcac gcaaaccctt
      961 cgcggtttat acgtgggtcg accgcggcca ctaccgcgcc ggcgacacga tcgaggcgca
     1021 agttcatgcc cagacactcg accataagcc cgtagccggc aaggggacga tcaagctcct
     1081 caaaatcgac tacggccaag agcgcaggcc ggtcgaaaca caggtcgaaa gctgggagct
     1141 ttcgctcgat cccgcagggg ttgcgcggca aactctcaag gccgcggcgg caggccagta
     1201 tcgcttggca gccacaatcg aagacggcaa aggccacgcg atcgaggggg gctatgtgtt
     1261 cacaatcacc ggccagggat tcgacggcgc gagttaccga ttcaatgacc tcgaggtcat
     1321 ccccgatcgc aaggagtatc gcccgggcga gaccgtccgg ctcttgatca acaccaatcg
     1381 ggtcaattcg acggtccttt tgttcgtccg gccgacgaat ggcgtgtatc tcgagcccaa
     1441 ggtggttcgc ttgcagggca agagcacggt cgaggagatc ggcattgcgc cgcgcgacat
     1501 gcccaacatc tttgtcgaag cgctcacggt cgccgacggc aaggtacacg atgaggtgcg
     1561 cgaaatcgcc gtaccgcccg agtcgcgcat cgtcgacgtt gcgatcgagc ccgcccagac
     1621 gtcgtacaaa cctggccaga gagccaaggt caaggtcaag ctcaccggcc ccgacggcaa
     1681 accgtttgag ggctcgtcgg tgctgacggt gtatgacaag gcggtcgaat acatctcggg
     1741 cggatcgaac gtaccagata tcaaagaatt cttctggaaa tggaagcgtt accaccagcc
     1801 tcagaccgag tcgagcctcg accgctggtt ttctaacctg acgaaaccga acgaggtggg
     1861 aatggaagac ctgggtggga cagacggact ggaaccgcag ttccaaccgg gtgccgtcgc
     1921 gtacaggaag cggcgatcgg cgatgcccat gagagggatg gcggggatgg gcggcaatag
     1981 gggggttgaa tttgacctcg caatgcccgc tgccgttatg gctgcgccca tggcgaaggc
     2041 ggatgcagcg gtgggcggcg agcgtcagcg gcatgacgtg atttccgcgg ac
//
LOCUS       DATAQL010000673         4554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3887633, whole genome shotgun sequence.
ACCESSION   DATAQL010000673 DATAQL010000000
VERSION     DATAQL010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3887633"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VHS97_11775"
     CDS             complement(<1..918)
                     /locus_tag="VHS97_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448928.1"
                     /translation="MITCTRNRVGSHLFGIMILGAIGAWSQTNGADVLPPPAASTAAK
                     GAIEPVAPVVPSEVVAALQQGDHETARKALIALGDKSKDRDEVSYYAYLRAIAERLAG
                     RRDAARETLGTALKANPVGRWAAKIRLELAGIELAAGNWTVAEELTRAEAVRLLAGPR
                     KDQLAGVYSSFAQKMLEPGDPLVPADPNAAYELLTQARALAESPGLRAQLLFAMGRAS
                     LTAPNPRRAIENFQLYLKEYSDGADRLAVRFQLGEALRRANQIVPARIAWTDLARDIE
                     RKKPAEVSKVIAAIRADALYEIASTYGIPN"
     gene            1875..3155
                     /locus_tag="VHS97_11780"
     CDS             1875..3155
                     /locus_tag="VHS97_11780"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3448929.1"
                     /translation="MPTAIFLGCFWKTRIEDRDGDPPFSVGLLYGPSGCGKSSLIKAG
                     LLPRLADSVVAVFAEATPADTERRLAAAVVARCHGLSTELPLSELLGAIRRGQGLGRD
                     RKLLIVVDQVEQWLQANEITENAELVPAFRQCDGVRVHALLIVRDDFWMSVTRFFRML
                     DVRLSEVTNCTAVELFDIYHAENVLTAFGRAFGALPEAPATPMKDQKAFITQSVTELS
                     QNGQVIPVHLSVFAEMVKGQEWTPVTLNDLGGSRGLGVMFLDKAFGEGAPPYRRIHQR
                     AAREVVNRLLPASGVDIKGRVHSRSELLAASGYADRLDDFEELVRVLDSELKLVTPID
                     RSTIGGGEPHYQLTHDFLVPALRKWLAAKQRGTRSGRAELRLAERAALWSDRCEPKQP
                     PTIVEWLTITLLTPRHRWDRSERLRSIRLTVVDP"
     gene            complement(3159..4307)
                     /locus_tag="VHS97_11785"
     CDS             complement(3159..4307)
                     /locus_tag="VHS97_11785"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX3448930.1"
                     /translation="MSMPTFNVTSHLDSPALTAGDGTHRGEILAANLVGAGNSALPNN
                     PRIDNNAGQKYPVIRAVVIAHNGAHVTGTFRSTPSTTFTIEFFANATGDPSGYRQGQT
                     YVTSISVTTDGGGNASFNNVPLGQIALGSAVSATATDNTSFNTSEFAQDAIATTTTAT
                     AVSSSSSTSTLGQIVTFTAAVTPSAGGGPSSGTVTFYDGTQVLGTGTLVSPVVWTFST
                     AGLPLGPNTITFTYSGNANIASSTTPASTQTVLAPSNTTVTPSTSTAVVGQSVILNAT
                     VTVPAGTGTPTGTVTFIDVTDPTNAVALASVPILANANLFIGGTVMGTATLDGNGKAS
                     MTVSSLPLGQSLDVLERAVRFARSAPARSGGSRRREERVCLRTRGANP"
     gene            complement(4374..>4554)
                     /locus_tag="VHS97_11790"
     CDS             complement(4374..>4554)
                     /locus_tag="VHS97_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448931.1"
                     /translation="PCIQSFCNPDARSGGPVEALTGHDDPARATDALRLLFFNVRSND
                     VSLSTIASPVARSET"
BASE COUNT          864 a         1306 c         1455 g          929 t
ORIGIN      
        1 attggggatg ccataggtcg atgcgatttc ataaagtgca tcggcacgaa tggcggcgat
       61 gactttggag acttcggctg gctttttgcg ttcgatgtcg cgggcaagat cggtccacgc
      121 gatgcgggcc ggcacgattt gattcgcccg gcgcagggcc tcgccgagct ggaatcggac
      181 ggccaggcga tcggcgccgt cgctatactc cttgagatag agctgaaagt tttcgatcgc
      241 acggcgcggg tttggtgcgg tcaggctggc gcgacccatc gcgaacaaga gctgggcgcg
      301 caggcccggg ctttcggcga gcgcgcgtgc ttgggtcaat agctcataag ccgcgttggg
      361 gtcggcggga accagcgggt cgccaggttc gagcattttc tgggcgaagc tcgagtacac
      421 cccggctagc tgatctttcc gaggcccggc caaaaggcga acggcctcgg cgcgcgtcaa
      481 ctcttcggca acggtccagt tgccggcggc cagctcgatg cccgcgagct cgagtcggat
      541 cttggcggcc cagcggccca cggggttggc ctttagagcc gtgcccaaag tctcacgagc
      601 cgcgtcgcgc ctgcccgcga gccgctcggc aatcgcgcgg aggtaggcat aatagctgac
      661 ttcgtcgcgg tctttgcttt tatcgccaag cgcgatcagt gcttttcgcg cggtctcatg
      721 gtcgccttgc tggagcgcgg ccacgacctc cgacggcacg acgggcgcga cgggctcgat
      781 cgcgcctttg gccgcggtgg aagcggccgg aggtggcaag acatcggctc cgttcgtttg
      841 cgaccaggct ccgatcgctc cgagtatcat gatcccgaac aaatggctgc cgacccggtt
      901 cctggtgcac gtgatcacgg ctgagcctcc ttcagagatg caagttacgt cttgccggtc
      961 gatttggtgc cgcacgagac actgcagaag acgatcctga atccaacagt ttgcagcgca
     1021 tgactcccca gacgttggat gtatggcggg acgaattcgt tccgccccgc gcatggcaga
     1081 cgagcaattt ccaccgacat ggatctttgt cgaaacctcg agggaacgag cagatgcaaa
     1141 gccatgatgc cggacgcgcg tctcagcgga gaatcatttt ttgaccacgg aacgcgcgga
     1201 aaagcacgga aacagtaagg agttatagaa ctctccgttc aacctctgct tgtggatagc
     1261 tcaattgtac ctgggacaga taaggcagat ttctgagctt gaactccttt tcgagacatt
     1321 cttggtatac gggcgcgagg aacctacaac ccacttctcg atagacttcg aatatggccc
     1381 cgcggattgc gtacgactct tcggcataaa ggatctcgcc cattttctgc ttctctcaga
     1441 attccccgag cattgctcgg ttctcggatg tcccacccat cctaacgtac gcgatcatca
     1501 gtcggtggta gaacgaattc tttaaccgcc gaaggcacag acgaccaggg aaaatccaat
     1561 gcggaggaat gtccggctcc atctcgtgtc gcaattctca gctcaccaat ggaacgaatg
     1621 ctttgaccac gaaaggaagg gaaaatcacg gaaaaagtct cgggtgaaag aattgttggt
     1681 ccaaccgcgc gttgggatag gtgtcgcgcg actttaatga gcttcggccc aactctctat
     1741 gaattccgaa ctcgggcccg cttatcgcgt aggactatca acaaattcga gcttttccgt
     1801 ttccgggcct ttccgagctt tccgtggtta atggtttgtg tccgcattca cgagttcgcc
     1861 tcgcaactta taacgtgccc actgcgattt tcttaggctg cttctggaag acccgaatcg
     1921 aggatcgcga cggtgatcca cccttcagcg tgggccttct ttacggcccg tcggggtgtg
     1981 gcaagtcatc gctgatcaag gccggtctgc tcccccggct agcggactcg gtcgttgccg
     2041 tgttcgctga agcaacaccc gccgacaccg agcggcggct cgcggctgct gtggtggcga
     2101 gatgccacgg gctgtcgacc gagttacccc tctctgagct gctgggcgcg atcaggcgcg
     2161 gccagggact tggccgtgac cggaaattgc tcatcgtggt cgaccaggtc gaacaatggc
     2221 tccaggcgaa cgagatcaca gagaacgcag agttggtccc ggcgtttcgt cagtgcgacg
     2281 gcgtccgggt tcatgcactt ctgattgtca gagacgactt ctggatgtcg gtgacgcgtt
     2341 ttttccggat gctcgacgta cgcctttcag aggttacgaa ttgcaccgcc gtggaactat
     2401 tcgacatcta ccacgcggaa aatgtgctca ccgcgttcgg gcgagccttc ggcgcactgc
     2461 cggaggcccc ggcgacgccg atgaaagacc agaaggcttt cataacgcaa tcggtcaccg
     2521 agctgtcaca aaacggtcag gtgatcccgg tgcatctcag cgtgttcgcc gagatggtca
     2581 aaggacagga atggacgccg gtcacgctca acgatctcgg cggctcgcgc gggctgggtg
     2641 tcatgttcct cgacaaagcc ttcggagagg gcgcgccgcc gtatcgtcgg attcatcagc
     2701 gtgccgctcg cgaggtcgtc aatcgcctgc tgccggcgag tggagttgac ataaaaggac
     2761 gcgtgcattc gcgcagcgaa ttgcttgctg cgtccggcta cgccgatcgg ctggatgact
     2821 ttgaagagct cgtgcgcgta ttggactccg agttgaaact cgtgacgccg atcgatcgca
     2881 gcacgatcgg cggcggcgaa cctcattatc agctcacaca tgatttcctg gtccccgcct
     2941 tgcgcaagtg gctggcggcg aagcaaaggg gcacgaggag cggccgggcg gagctccgac
     3001 tcgcggagcg tgccgcgctg tggagcgacc ggtgcgagcc gaaacaaccg cccacgatcg
     3061 tcgaatggtt gaccatcacc ttgctcacgc ctcgccaccg ctgggaccgg tcggaacgct
     3121 tgaggtcgat ccgcttgacc gtggtcgatc cataggggtt agggattggc accgcgcgtt
     3181 cgaagacata cgcgttcttc tcgtcgtcgg gacccgccgg atcgggccgg ggcacttcga
     3241 gcaaatcgca cagctcgttc aagaacatcg agtgattggc cgagcggcaa cgacgaaacg
     3301 gtcatgctgg ctttgccgtt cccgtccaac gttgctgttc ccatcaccgt gccgccgatg
     3361 aacaaattgg cattcgccaa aatcggcacg ctggcgaggg cgacggcatt ggtcggatcg
     3421 gtaacgtcga tgaacgtcac ggtcccagtg ggcgtgcccg tgcccgccgg cactgttacc
     3481 gtcgcgttca agatcaccga ctggccgaca accgccgtcg aagttgatgg cgtcacagtt
     3541 gtgttgctgg gcgccagcac cgtttgggtc gaggccggtg tggtgctgct ggcgatattc
     3601 gcattgccgc tataagtgaa ggtgatggtg ttgggcccga gcggcaggcc ggcggtcgag
     3661 aaggtccaga cgacggggct gacgagcgtc cccgtgccga gcacctgggt gccatcgtag
     3721 aaggtgaccg tgccgctcga tggaccgccg ccagcactcg gcgtcacggc ggcggtgaac
     3781 gtgacgatct gaccaagagt gctggtcgac gaagacgagc tcacggcggt tgcagtcgtt
     3841 gtggtcgcga ttgcgtcttg cgcgaattcc gacgtgttga aactcgtgtt gtccgtcgct
     3901 gtggccgaga cggccgaccc gagcgcgatc tgaccgagcg ggacgttatt gaaactggcg
     3961 ttgccgccgc cgtcggtcgt cactgatatc gacgtgacgt aagtctgccc ctggcgatag
     4021 ccggaagggt cgccggtggc attcgcgaaa aactcgatcg taaatgtcgt gctgggcgtg
     4081 ctgcgaaacg tacccgtcac gtgcgcgccg ttgtgggcga tgacgaccgc gcggatcacc
     4141 ggatacttct ggccggcgtt attgtcgatc ctgggattat tgggaagcgc ggaattgccg
     4201 gcacccacga ggttggccgc gagaatttcg ccgcgatggg tgccgtcgcc agccgtgagg
     4261 gctgggctgt cgaggtgact cgtcacgttg aacgtaggca tggacatgag ctgtaatttt
     4321 cgaggatctc gagcaatggc cgggaacggc ggcgggccgc attgcgacga agttcaagtt
     4381 tccgagcgag caaccggcga tgcaatcgtg ctgagcgaga catcgtttga cctcacgttg
     4441 aaaaatagaa ggcgcagcgc gtccgtggcg cgcgcggggt cgtcgtggcc ggtcagagct
     4501 tcgacaggac ctccggatct ggcatcggga ttgcagaagc tttggatgca tggt
//
LOCUS       DATAQL010000674         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1901700, whole genome shotgun sequence.
ACCESSION   DATAQL010000674 DATAQL010000000
VERSION     DATAQL010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901700"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2075
                     /locus_tag="VHS97_11795"
     CDS             <1..2075
                     /locus_tag="VHS97_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448932.1"
                     /translation="AILVVLDWWFRPGTTARIILLELVLIGLVPFLLIRAFRRWRAAR
                     LDDLSLAMVLDRFRPGTGGRIADVLQLPEQLGEPATTVSPAMIRLAVKHATEALAASD
                     WASLWNRGRTTKRSSALLIALLVPTLFAALAPHAARLSVARWLLGSSERWPQRTYLTV
                     TGLGDRKRLVAPRDEPFALEVRADLPALEPRDGKWIVRGRGEPLLLRNRPQAPEAPTA
                     VRLRERTAKGGIRDTVMTVTGPASFRHELPPSSSSTTFNLSGGDDWLGPIKVERVDRP
                     ALASARLRVREPGSKGDTFRVVEESIQQPVFLPDTEVELTLAGNEPISEARLTVHPGE
                     APKLARVDARTFVARWILREATTLEVELTSEATSLTSKTAYLSIGILKDREPRVSLRA
                     LGVGAHVTSVATIPLSITATDDMGLAVVRLQVERTTTEGDKGEPKTTKKTVPLPLAAD
                     TARAILDHQARHDVELQADTPPIGTVLRFVAEAEDRCARGVQVGRSTALQMQVVSPNE
                     LYYEILIRQRAERAKFVSLIESAEKQTPLLAGSPTPDGYAGVMRGLHASSRQLELIAG
                     RIVDTLQEMKLNQVGSPKAYRLLQEGVIDPIRALNAGPMSELRVVLQSLAGPGSKLDA
                     NPETARRLHAGVVTEMKNILEQMSQWESFVDVVNQVAEVIKMQHNVLDAAEKARESRT
                     QEIFDGKP"
     gene            2062..>3320
                     /locus_tag="VHS97_11800"
     CDS             2062..>3320
                     /locus_tag="VHS97_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448933.1"
                     /translation="MGSRSKHPEQLRVISGGRGSARLSSPKSVRAGWRRGSPGGSPSQ
                     DRAQAFSWEKLAPLPLLIATVGFILSPPALAQATKAATKAAAVFDEEKKTAPKPATVS
                     DRDTIGFTQENAAAQMTELEERMFRLSEALRGLEPENASRLRLALKFSREEQILDQMR
                     ETHKFLKDAQLSKAETEVKELIAKLEHLRTLLLAEDLDFQLKLARLRQMRETANQLDR
                     IAKEERRELGWTRFAMEERRRLERIATRRPDLEALIRDQQAVIADTKNTSKDGEKSAK
                     ETCTAIGERERKLKSAVTALASDPLFADLQPPHLRRSDALLADAVTSLEGADSSAAAL
                     AEDKALGLVREELTRLDDQVVETERTIAESEFKRREGDQVKNRGTTDRLAESSARLGD
                     AGVALRKDLIRASASMRSAEQGLGKAAA"
BASE COUNT          632 a         1049 c         1066 g          573 t
ORIGIN      
        1 cggcgatact ggtggttctc gattggtggt ttcggcccgg cacgactgcc cgcatcattt
       61 tactagagct cgtccttatt ggtcttgtac ccttcctctt gatccgcgcc ttccgtcgct
      121 ggcgggcggc tcgtctcgac gatctctcac tggcgatggt gctcgaccgc ttccggccgg
      181 ggactggagg gcggattgcc gacgtgctcc agctccccga gcaactgggc gaacctgcga
      241 ccacggtgtc accggcgatg atacggctgg ctgtcaaaca cgccacggaa gcgctcgccg
      301 cctcggattg ggcatcgctc tggaatcggg ggcgaacgac caagcgcagc tcggccctct
      361 tgatcgccct gctcgtcccg accctgttcg cggcgcttgc tccgcacgcg gcccggctga
      421 gcgtggcacg ctggcttctc ggctcgtcag agcggtggcc gcagcgcact tacctcacgg
      481 taaccgggtt gggcgaccgc aaacggctag tggcaccccg agatgagccg tttgcgctcg
      541 aggttcgggc cgatttgcca gcgctcgagc cccgtgatgg taagtggatc gttcggggac
      601 gcggcgagcc gctcttgctc cgcaaccggc cccaggctcc ggaagctccg accgccgtcc
      661 ggctccgcga gcggaccgcc aagggaggga tccgcgatac cgtgatgacc gtgactgggc
      721 ccgcgagctt tcgccatgag ttgcctcctt cctcgtcgtc gaccacgttc aatttgagtg
      781 gaggagatga ttggctcggt cccatcaagg tggaacgcgt cgaccggccg gcacttgcga
      841 gtgcgcggct gcgggtccgt gaaccggggt caaagggaga cacgtttcga gtcgttgaag
      901 aaagtattca gcagccggtc tttctgccgg acacggaggt cgagctgacc cttgccggca
      961 acgaaccgat ctcggaagcc cgactgaccg ttcaccctgg cgaggcgccc aagctggcaa
     1021 gggtcgacgc gcggacgttt gtcgcgcggt ggatcctgcg cgaggcgaca acactggagg
     1081 tcgaactgac ttccgaagcc accagcctga cgtcgaagac cgcctatctt tcgatcggga
     1141 tcttgaagga cagagagccg cgcgtttcgc ttcgagctct cggggtaggc gctcatgtca
     1201 cgtccgtcgc caccattcca ctttcgatca cggcgaccga cgatatgggg ctcgcggtcg
     1261 ttcgcttgca ggtcgaacgg acgacgaccg agggggacaa gggcgagcct aagacaacca
     1321 agaagactgt tccgctgccg ctggccgccg acactgctcg agccattctc gatcatcaag
     1381 ccaggcacga cgtggaatta caggccgata ctcctcctat cgggacggtc cttcggttcg
     1441 tggcggaggc cgaagaccgc tgcgcccgag gggtccaggt tggccgatcg acggccctgc
     1501 agatgcaagt cgtttccccg aatgagcttt attacgagat cttgatccgc cagcgcgccg
     1561 agcgggcgaa atttgtctct ctgatcgagt cggcggagaa acagactccg cttttggcgg
     1621 gctcgccgac acccgacggc tacgccggcg tcatgcgcgg actgcacgcg tcgtcgcgcc
     1681 agctcgaact gatcgccggc cggattgtcg acacgctcca ggagatgaag ctgaaccagg
     1741 tcggatcacc caaggcttat cgcctgctgc aggaaggcgt gatcgacccg atccgcgctt
     1801 tgaatgccgg cccgatgagc gaacttcgtg tcgtgctcca gtcgctggcg gggcccggtt
     1861 caaagttgga cgccaatccg gagacggccc gccgcctgca cgcaggcgtc gtgaccgaga
     1921 tgaaaaacat tctcgagcag atgtctcaat gggaaagttt tgtagacgtc gtaaatcagg
     1981 tggccgaagt gatcaaaatg cagcacaatg ttctcgatgc cgccgagaaa gcccgcgagt
     2041 cgcgcacgca ggagattttc gatgggaagc cgtagcaagc atcctgagca gcttcgcgtg
     2101 atttcgggag ggcgaggctc ggctcgactg agctcgccga agtccgtccg agccggatgg
     2161 cgccgcggct cgcctggagg ctcgccctcc caggatcgtg cacaggcatt tagttgggaa
     2221 aagttggccc cgctgcctct cttgattgcc acggtcggct ttatcctctc gccgcccgca
     2281 ctcgcgcagg ccaccaaggc agcgaccaag gcggccgctg tcttcgacga ggagaagaag
     2341 acggcgccca aacctgcgac cgtgtccgac cgcgatacga tcggatttac tcaggagaac
     2401 gccgccgccc agatgaccga actggaagag cggatgttcc ggctctccga ggctctgcgc
     2461 gggctcgagc ctgaaaacgc gtcgcggctt cgtctcgccc tcaagttctc gcgcgaggaa
     2521 cagattctcg accagatgcg cgaaacgcac aagttcttga aggatgccca gctcagcaag
     2581 gccgagacgg aggtgaaaga gctcatcgcc aagctggaac acctccgcac gctgctcttg
     2641 gccgaagacc tcgacttcca gctcaagctg gcgcggctgc ggcagatgcg cgagacggcc
     2701 aaccagcttg accggatcgc caaggaagaa cggcgcgagc tgggatggac tcgctttgcg
     2761 atggaagagc gcagacgact ggagcgaata gcaacccgtc ggcctgacct ggaggcgctc
     2821 atacgcgacc agcaggctgt gatcgccgac accaaaaaca cgtcaaaaga cggagaaaag
     2881 tcggccaagg aaacatgcac agcgattggc gagcgcgagc ggaaactcaa gagcgcggta
     2941 accgcactgg cgtccgaccc gttgttcgcg gaccttcagc cccctcatct acggcggtcc
     3001 gatgcgctct tggccgacgc ggtgacatcc ctggaagggg ccgactcaag cgccgccgcc
     3061 cttgccgagg ataaggccct tggcctcgta cgcgaggaac tcactcgcct ggacgatcag
     3121 gtggtagaga ccgagcgcac gatcgccgag tccgaattca agcgccgcga aggggatcaa
     3181 gtcaagaacc gcggcacgac cgataggctc gcagagtctt ctgcacggct gggcgacgcc
     3241 ggcgtggcgt tgcgaaaaga cctgatccgc gccagcgcct cgatgcggtc ggccgagcag
     3301 ggtctcggca aagccgcggc
//
LOCUS       DATAQL010000675         4636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_681090, whole genome shotgun sequence.
ACCESSION   DATAQL010000675 DATAQL010000000
VERSION     DATAQL010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681090"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VHS97_11805"
     CDS             <1..792
                     /locus_tag="VHS97_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448934.1"
                     /translation="SRGENGAGRIGLVLDTADCLTGFADAQLAGGDRVEFLPSLRVIG
                     GRELRGGSDWLRQLEAPGVMIDPLVLVAGSDGQLDEQAEAIRRLVLRHNPATIICVDR
                     YSLASIRSGSHGILHALIPDVVVFDESFAGRAVPFAAFTARRSLFAAWNRPGKSTFHS
                     TTFQPNTITTRHFMNVLAMTDPGFCQQYAEELRAIACNLVRRGDAFRRYYQPSLYRLI
                     RAAGFDTSDLRASGSFVFVDGQPILDFVGGVACSVRGHNPPSYAD"
     gene            793..1905
                     /locus_tag="VHS97_11810"
     CDS             793..1905
                     /locus_tag="VHS97_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3448935.1"
                     /translation="MRSLPARGVDNSSLEAELGGRLQDLTGLGHVLPAVSGANAVENA
                     LRLALVARFPRRHVLALKAGFGGKTLFSLTGTANPSYKKRVGPLYADVHYVDPFAPDA
                     RDQIDILFREHEFAVVQVELIQSVGGVRRIPQALVRHLAEGRERWGYLLIVDEIQTGM
                     YRTGPFVQSRTFGLTPDFLLLGKGTSDMMFPFSLTLYSDAAAVLLAERGSSLIDAIRH
                     RYGYELGYKTVLNVLKSAERLGLSEHVADAGAQFERLLNDGLGPLEIVREVRVFGLLI
                     AIELEASSGPRRWLRKGLSTPYLLAMLHHEAFPVMAGFCQYEPNTLKITPPLNVCRDE
                     IVQSCKTMTDVLSRPLLRVLPVALRRMLKPLPIRKG"
     gene            1908..2441
                     /locus_tag="VHS97_11815"
     CDS             1908..2441
                     /locus_tag="VHS97_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448936.1"
                     /translation="MSAGIIQRLSLLRFDFDGLYIRHLGRHSQLGINIAHLIALYGIW
                     FGIYAVIGQGVLLMGLPSAWPVLVPMALAYLATISMNSPFSVTVATAAFVGLLVGSVL
                     ALPALPLWSIIGFLALLPVSYKVQAYSHKVWTIAADMSEHNKRFPPGRELNIILLFYE
                     VPICLKYLLFCPGDWNR"
     gene            complement(2550..3365)
                     /locus_tag="VHS97_11820"
     CDS             complement(2550..3365)
                     /locus_tag="VHS97_11820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3448937.1"
                     /translation="MSKTSIHHAYNDIVATHYDLDPQGLIGESLDRGIAQMKAEGFFD
                     SDRELHVLDVGMGTGLFLDKLKKLAGDRVVPFGIDLADNMLEVARRKMPDLEAIAGDA
                     SSLEDYFPGKDFDCVCTHFMTGYVGMNVLAPQIARRLKPGGYWSLIGGTKKAYSALQA
                     KGDSKVLRWLSGVGSREMDDTVLNPADLAEVSKTMAAHGLEVRQGETFEPALKFENFD
                     TFMEFGYRGGWLTPLIESTGLHEAGPAKRWLINRLMFPVRDAHNIVLALGQKP"
     gene            complement(3505..4326)
                     /locus_tag="VHS97_11825"
     CDS             complement(3505..4326)
                     /locus_tag="VHS97_11825"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX3448938.1"
                     /translation="MQADTEQIRETRPKTYACLIISDLHLGSDVCQAALILQFLEWAV
                     ENTDELIINGDIFDDLNFNRLSKRHFATLKTIRRNSDREDLRLVWVRGNHDGPADVVG
                     HIVGVEVLDEYTFENESLRLLVLHGDQFDRFVNDYGWLTEIACALFYYIQKWAPHQTA
                     RYIRRISKRWQRSSILIRDGAVTYAASKGYRYVSCGHTHLPFIEQVGDVLYVNSGTWT
                     EHPPCPFVSVKGDEIRLEFWPIATEELRGLSEVVDDGVSETASSMILEDKSTQFR"
BASE COUNT          891 a         1387 c         1309 g         1049 t
ORIGIN      
        1 agcagagggg aaaatggagc cggccggatc ggcctcgtcc tcgacaccgc cgattgcctc
       61 accggcttcg cggatgccca gcttgccggc ggcgatcgcg tcgaattctt gccgagcctt
      121 cgcgtgatcg gcgggcgaga actcagagga ggctccgatt ggctgcgtca gctcgaggcg
      181 cccggcgtga tgatcgatcc gctcgtgctg gtcgctggaa gcgacggcca acttgatgaa
      241 caggccgagg cgatccgccg gctggtgctt cgccacaacc cggccacaat catatgtgtc
      301 gatcgataca gccttgcctc gatccgaagc ggctcacacg gtatcttgca tgcgttgatc
      361 cccgacgttg tcgtcttcga cgagtcattc gcgggccgag ccgttccgtt cgccgccttc
      421 acggcccgga ggtcgttgtt cgccgcgtgg aaccggcctg gcaagagtac ttttcactcg
      481 acgacgttcc agccgaatac gatcaccacc cggcatttca tgaacgtcct cgccatgacg
      541 gatcctggct tttgccagca gtacgccgag gaacttcgag cgatcgcctg caatcttgtt
      601 cggcgcggcg acgcctttcg gcgctattac caaccctcgc tctatcgact gatccgcgct
      661 gcggggttcg atacgagcga tctacgggct tcgggctcct tcgtgtttgt ggacggtcaa
      721 cccattctcg actttgtggg cggtgtcgct tgtagcgtgc gaggccacaa tcctccctcc
      781 tacgcggact aattgaggtc gctaccagcg cgtggcgttg acaactcctc ccttgaagcc
      841 gaactcgggg gtcggctcca agatctcacc ggcctcggac acgttttacc ggcggtcagc
      901 ggggccaacg ctgtcgagaa cgccctacgg cttgccctag tcgcccgttt tcctagacgg
      961 cacgtccttg ccctcaaggc cggctttggt ggcaagaccc tcttctctct cacgggcacc
     1021 gccaacccct cgtacaagaa acgcgttggc cctctgtatg ccgacgtcca ttacgtcgac
     1081 cccttcgctc ctgacgccag ggatcagatc gacatactct tccgcgagca cgaattcgcc
     1141 gtggtccagg tcgagttgat tcagtccgtc ggcggtgtgc gacggatccc gcaagcgctg
     1201 gtgcgccatc tcgccgaggg aagggagcgc tggggctatc tgctcatcgt ggacgaaatc
     1261 cagactggca tgtatcggac cgggccattt gtccaatcgc ggactttcgg cctgacgcca
     1321 gactttttgc tgctgggcaa ggggacatcg gacatgatgt tcccattctc actgaccctc
     1381 tactccgacg cggcggcggt gctgctcgca gaacgaggct cctcgttgat cgatgcgatt
     1441 cgccaccgct atggctatga gttgggttac aaaacggtgc tgaatgtctt aaagtcggcc
     1501 gaaagacttg gcttatccga gcatgttgcc gacgccggtg cgcagttcga gcgattgctc
     1561 aacgacggtc ttggtcctct cgaaatcgtc cgcgaggttc gcgttttcgg gctactgatc
     1621 gccatcgaac tcgaagcgag tagcgggccc cgccgttggc tccgaaaagg cctctcgact
     1681 ccttacctgt tggccatgct ccatcacgaa gccttcccgg tgatggcagg cttttgccag
     1741 tacgagccga atacactgaa aatcactcct ccacttaacg tctgtcgcga tgagatcgtt
     1801 cagtcctgta agacaatgac tgatgtgctg agtcgcccgc tcttgagggt gctaccggtc
     1861 gccctccgtc gtatgctgaa gccgcttccg atccgaaagg gataaacatg agcgcaggga
     1921 tcatccagcg tttgagcttg ctccgcttcg acttcgatgg cctgtacatc aggcacctcg
     1981 gtcgtcattc ccagttgggg atcaatatcg ctcacctgat cgcactttac gggatctggt
     2041 tcggcatcta tgccgtgatc ggccaagggg ttctcctgat gggcttgccg tcggcgtggc
     2101 cggtcctcgt gcccatggcg ttggcctacc tcgccacgat cagtatgaat tcgccctttt
     2161 ccgtgacggt cgccacagcg gcgttcgtgg gcctgctggt ggggagtgtc ctggcgctgc
     2221 ccgcactccc gctctggtcg atcattggct ttctcgcact gctccccgtc tcttacaaag
     2281 tgcaggccta tagccacaag gtgtggacaa tcgccgcgga tatgtccgaa cataacaagc
     2341 gattcccgcc gggacgcgaa ctcaacatca tcctgctctt ttacgaggtc ccaatctgcc
     2401 tcaagtatct cttgttctgt ccgggcgact ggaatcgctg attggtcaaa agacaagcgt
     2461 cgcgagggaa gttgggccgc gaaggatggt tccgatgatc agagcttcgt ccctccgggt
     2521 tcctcgcgac aaaccgattc ggttttcgtt tagggctttt ggcccagggc gagcacaatg
     2581 ttgtgtgcat ccctgaccgg gaacatgaga cgattgatca gccatcgctt cgccggtccg
     2641 gcctcgtgca aacccgtgct ctctatgagg ggagtgagcc agccgccgcg ataaccgaac
     2701 tccatgaagg tgtcgaaatt ctcgaacttc agcgctggct cgaacgtttc gccttggcgg
     2761 acttccaggc cgtgcgcggc catcgtcttc gagacctctg ccaggtccgc cgggttcagg
     2821 acagtgtcgt ccatttcgcg cgacccgaca ccgctgagcc atcggaggac cttggaatca
     2881 cccttcgcct gaagggcgga gtaagccttc ttggttccgc caatcagcga ccagtagccg
     2941 cccggcttaa gccgcctggc gatctgcggc gcgagcacgt tcatccccac atagcccgtc
     3001 atgaaatggg tgcagacgca gtcaaaatct ttacctggaa aatagtcttc cagacttgag
     3061 gcgtccccgg cgattgcctc taggtccggc atcttccggc gtgcaacttc tagcatgttg
     3121 tcagccaagt cgatgccgaa gggcacgact cgatcgccgg cgagcttttt gagtttgtcc
     3181 aggaagagcc cagtccccat tcccacgtcg agcacgtgca attcgcggtc ggaatcgaaa
     3241 aatccttccg ccttcatctg agcgatcccg cgatcgaggg actccccgat caacccttgg
     3301 gggtcgaggt cgtagtgcgt cgcgacaatg tcgttgtacg catgatggat agacgttttg
     3361 ctcatgacga accgcgatcc ccttctgact ggccgataaa gttaaaagtc ggcttccgtt
     3421 tcatgcacac ccgccacggg attaacggcc caacctggga gcgagctctg tgaagttcag
     3481 tcggttcaaa cgacgtttgg aagattacct aaattgagtc gacttgtcct caaggatcat
     3541 cgaggaggca gtttccgaca cgccatcatc gacgacttcc gacaggccgc gtagctcctc
     3601 cgtcgcgatc ggccagaact ccaggcggat ctcatccccc ttgaccgaca cgaacgggca
     3661 aggaggatgc tcggtccagg taccactatt tacgtagagc acatcgccta cctgctcgat
     3721 gaaaggtaga tgagtgtggc cgcagctgac ataacggtag cccttcgagg ccgcgtaggt
     3781 cacggcaccg tcacggatca gtatgctgct gcgttgccac cgcttcgaaa ttcgccggat
     3841 gtagcgggcc gtttggtgag gtgcccattt ttggatgtaa taaaacagcg cgcaggcgat
     3901 ctccgtcagc cagccgtaat cgttgacaaa acggtcgaac tggtcgccat ggaggacaag
     3961 caggcggaga ctttcgttct cgaacgtata ctcgtcaaga acctcgacgc caacgatatg
     4021 gccgactacg tcggctggtc catcgtggtt gcctcggacc cacaccaatc ggagatcttc
     4081 gcgatcggag ttacggcgga tcgtctttag ggtggcgaag tgacgcttgg agaggcggtt
     4141 gaagttcagg tcgtcgaaga tgtcgccgtt gatgatcaat tcatcggtgt tctccaccgc
     4201 ccactccagg aactgcaaga tgagcgcggc ctggcagaca tcactaccca ggtgcagatc
     4261 gctaatgatc aggcaagcgt aagtctttgg ccgggtttct cggatttgtt cagtgtctgc
     4321 ctgcatggcg aagctaatct cgacgaattt tcaggtctcg gcccggggcc gatcggcctt
     4381 tcggccgggg tctacgtact gcatcgatcg gagatttcag cctggcactc gggcttcgat
     4441 tcgattcgga aacttcgaat agaaagttgg tcgctcgcga tcactcaatg ctgctcgttg
     4501 gtattcatga tcggagtatg accgaccatc ttttaacggc caagtagcta gactgtttct
     4561 cacaagagct tcacgacatt ttcatcttag tggattgcaa caaataacat aaagctactt
     4621 agatattaaa atatat
//
LOCUS       DATAQL010000676         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1749936, whole genome shotgun sequence.
ACCESSION   DATAQL010000676 DATAQL010000000
VERSION     DATAQL010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749936"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VHS97_11830"
     CDS             complement(<1..508)
                     /locus_tag="VHS97_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3448939.1"
                     /translation="MRPQFPGMDPWLEHPDLWPDVHNSLITAIRDELMPKVLPRYVVR
                     VESRTTVLTGLDVDLLYRPDLAVRAANLSALAVDRGVAVLERTQERHFEVIVPLIDQD
                     EIEETFLTIQELPDRKLVTAIEVLSPTNKKTTDARAEYLGKRRNLIRSGVNFVEVDLL
                     RSGEPMPVQ"
     gene            complement(518..>3005)
                     /locus_tag="VHS97_11835"
     CDS             complement(518..>3005)
                     /locus_tag="VHS97_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX3448940.1"
                     /translation="LVERMLESPHYGERWGRIWLDAARYADSDGYEKDKSRQVYFYRD
                     WVIGALNRDLPYNQFIIEQIAGDQLPGATQDQIVATGFLRNSMINEEGGIDPEQFRME
                     AMFDRLDCIGKGILGLTIQCAQCHSHKYDPLTHDEYYGMFAYLNNSHEANVAVYTREQ
                     QAQREKILGEIRDVEELIERHNPDWPARMAAWEKQVTVGQPSWEILRPEVDEESTGGE
                     KFYALDDGSLLAQGYAPTKHTVKMSVKTSTAPITAFRLELLNDPNLPMSGPGRSIKGT
                     GALTEFRVDAAPADGNGKPVAVKFSHATADVNPPVAALEPIFDDKRGKKRETGPVSMA
                     IDGKGETAWGIDDGPGRRNQPRQAVFALDKPIVFAGGTVLTVHLKQDHGGWNSDDNQN
                     NNLGRFRLAITTAPAAVVDPVPRHVREIMAIPRDKRTRAQTAEVFSYWRTTVSEWKEA
                     NAWIESIWKRHPEPSTQLALRERDQKRMTHLLQRGDFLKPGKTVEPGVPAFLNPLPPG
                     APTTRLTFARWLAARDSPTTARSLVNRVWQAYFGTGFVATSEDLGLQCDPPSHPELLD
                     WLAVEFMESGWSLKHLHRLIANSAVYRQSSRPTPEMLARDPYNRLLARGPRSRVDAEV
                     VRDIALAASGVLNSKVGGPSVFPPAPAFLFQPPTSYGPKVWNEATGPERYRRALYTFR
                     YRSVPFPMLQAFDAPNGDFACVRRTRSNTPLQALTTLNEPIYMECARALAFRALAEGG
                     KTDCDRLVYAFRRCMALTPTEPEISIVLDLLRRQTERFEKPQANPWTLAANDPAKPPL
                     LPAGVTPAQLAAWTAVSRVLLNLDETITKD"
BASE COUNT          561 a          921 c          919 g          604 t
ORIGIN      
        1 tttgcaccgg catcggttcg ccggaacgca ataagtcgac ctcgacaaag ttaacgccgg
       61 aacggatcaa gttgcgtcgc tttcccaagt attccgctcg tgcgtccgtc gtcttcttat
      121 tcgtcggaga caaaacctcg atcgctgtca cgagcttgcg atcgggaagc tcctgaatcg
      181 tcagaaatgt ttcctcgatt tcgtcttggt cgatgagggg tacgatgacc tcaaagtgcc
      241 tttcctgcgt tcgctcaagg actgccactc cgcgatccac tgccaatgcg gacaggttgg
      301 cagctcgaac cgccaagtcg ggtcgataca ggaggtcgac gtcgagcccg gtcaaaacgg
      361 tagtccgcga ctcgacgcgg acgacatagc gcggcaggac cttcggcatc agctcatcgc
      421 ggatcgccgt gatcaggcta ttgtgcacgt ccggccaaag gtcggggtgc tccagccacg
      481 gatccatacc cgggaattgt ggtcgcatcg gggacactca atctttcgtg atcgtctcat
      541 ccagattcaa caacacccgg gacactgccg tccaggccgc gagctgtgct ggcgtcacac
      601 ccgcgggcaa cagcggcggt ttcgctggat cgttggccgc caaagtccag gggttggcct
      661 ggggtttttc gaatcgttcc gtttggcggc gcagcagatc cagaacgatc gagatctccg
      721 gctcggtagg agtcagggcc atgcagcgac gaaacgcgta gaccagtcga tcgcaatccg
      781 tctttccgcc ttcggccagg gcccggaagg caagtgcccg cgcgcattcc atgtagatgg
      841 gctcgttgag ggtggtcagc gcttgcaaag gagtattcga tcgagtgcgg cgaacgcagg
      901 caaagtcgcc gttgggggcg tcgaaggcct gcagcatcgg aaagggcacc gaccgatatc
      961 ggaatgtgta gagtgcccgc cgatagcgct cgggaccggt cgcctcgttc cagaccttcg
     1021 ggccgtagct ggtcgggggc tgaaacaaaa aagcaggcgc aggcggaaac acactggggc
     1081 cgccgacctt ggaattgagc acgccgctgg cagccaaagc gatgtcgcgc accacctcgg
     1141 catcgacacg agaccgagga ccgcgcgcca gcagacggtt atacgggtcg cgcgccagca
     1201 tttccggcgt cggccgcgac gattggcgat agacggctga atttgcgatc agccgatgca
     1261 ggtgcttcag gctccatccg ctctccatga actcgacggc cagccagtcg agaagttctg
     1321 ggtgcgacgg cggatcgcac tgaaggccca ggtcctcgct ggttgcaacg aacccggtcc
     1381 cgaagtaagc ctgccatacc cggttcacca gggaacgcgc ggttgtgggg gaatcgcgag
     1441 cggccagcca acgcgcgaag gtcaaccttg tcgtcggtgc cccaggggga agtgggttca
     1501 agaatgccgg gacgccgggt tcgacggtct tgcccggctt gaggaaatcg ccccgttgca
     1561 gcaagtgggt catgcgcttc tgatcgcgct cccgaagagc cagctgcgtc gatggctccg
     1621 gatgtcgttt ccagatcgac tcaatccacg cgttggcctc cttccattcg gaaaccgtgg
     1681 tccgccagta gctgaatact tcggccgttt gggcgcgcgt ccgtttgtcg cggggaatcg
     1741 ccatgatctc gcggacatgc cgcggtaccg gatcgaccac ggcagccggc gccgttgtga
     1801 tcgccagccg gaaccgcccc aggttgttgt tctgattgtc gtcactattc cagccgccat
     1861 ggtcctgttt gagatgaacc gtcaagaccg tgcccccggc gaagacgatc ggcttatcga
     1921 gcgcgaagac cgcctggcgc ggctggttgc gccggccggg accgtcgtcg attccccagg
     1981 ccgtctcgcc cttgccgtcg atcgccatgc tgaccggacc ggtctcacgt ttcttgccac
     2041 gcttatcatc aaagatcggc tcgagcgccg cgaccggcgg gttgacgtca gcagtggcat
     2101 gggagaattt gaccgcgacc ggcttgccat ttccatccgc cggcgccgcg tcgactctga
     2161 attcggtgag agcgccggtc cctttgatcg aacggcccgg gccactcatc ggcaagttcg
     2221 gatcgttcaa gagctccaga cgaaacgccg tgatcggcgc ggtgctcgtc ttcaccgaca
     2281 tcttgactgt gtgctttgtc ggcgcatacc cttgggccag cagcgagcca tcgtcaagtg
     2341 cataaaactt ctcccctcct gtcgattctt cgtcgacttc cgggcgcaag atctcccacg
     2401 agggttgacc gacggtaact tgtttttccc acgccgccat ccgcgctggc caatcgggat
     2461 tatggcgctc gatcagctct tcgacatcac ggatctcgcc cagaatcttc tcccgctgcg
     2521 cctgctgctc gcgggtgtaa accgcgacgt tggcttcgtg ggaattgttg aggtaagcga
     2581 acatcccgta atactcgtcg tgcgtcagcg ggtcgtactt gtggctatga cactgggcgc
     2641 actggatggt taaacccagg atccccttgc cgatgcagtc gagccggtca aacatcgcct
     2701 ccatccgaaa ttgctcaggg tcgatccccc cttcctcgtt gatcatcgaa ttacgcaaaa
     2761 aaccggtggc gacgatctgg tcctgggtcg cgcctggaag ctggtcgccc gcgatctgtt
     2821 cgatgataaa ctggttgtac ggaaggtcac ggttgagtgc gccgatcacc cagtcgcggt
     2881 aaaagtaaac ctggcgagac ttatcctttt cgtagccatc ggaatcggcg tagcgggcgg
     2941 cgtcgagcca tatccggccc caccgctcgc cgtaatgggg tgactcgagc attcgctcga
     3001 cgaga
//
LOCUS       DATAQL010000677         5983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_528752, whole genome shotgun sequence.
ACCESSION   DATAQL010000677 DATAQL010000000
VERSION     DATAQL010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528752"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VHS97_11840"
     CDS             <1..466
                     /locus_tag="VHS97_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3448941.1"
                     /translation="LCPSEPRKTFWNQSPGPPVDPYPSADADYGGMYGPRALVNGFTN
                     NPPAGAMIFGQCIGLAQITDGASQTIIVGEDPEAINAMWVSGHNIFDQSAPINARPPS
                     EFGEELTSQHPGGVNVVFADGSVHFLKNSTALPPLAALCTRALGEVLSSDSY"
     gene            500..1381
                     /locus_tag="VHS97_11845"
     CDS             500..1381
                     /locus_tag="VHS97_11845"
                     /inference="COORDINATES: protein motif:HMM:NF026067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HEX3448942.1"
                     /translation="MTLRQPFVTLLGLGLAIALAAPGARGGDIVVNFNDLSYPNGSFD
                     PGEGFPAGPAPGTGSYDNGWDLNGGFTSNGTFFSNAYDTTFNSWSGWAYSNVNDPTTT
                     GPSPFLTDFNHQFAAITGTAPGGSGNYGISSGSGAYINLPSGTSPVSFEVTNTTYSFL
                     SMTLGDGFGKKFSTGDFFELKIFGYSGLNGTGTQVGEVDFFLANYTSANSLPVDVWTL
                     VNLTSLADAQSLGFDYASSDVGQFGINTPEFFAMDDLTLSTTSVPEPSSVILALAGLG
                     IVGLFASHRHWRSAVAR"
     gene            complement(1880..4648)
                     /gene="ligD"
                     /locus_tag="VHS97_11850"
     CDS             complement(1880..4648)
                     /gene="ligD"
                     /locus_tag="VHS97_11850"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX3448943.1"
                     /translation="MGLDEYRRKRDFHKTPEPRGKRAGRPHGQLAYIIQKHDASHLHY
                     DFRLELNGVLKSWAVPKGPDPDPAKKRLAMQVEDHPLEYGGFEGIIPEGEYGGGTVML
                     WDKGVWEPIGDAAKGLKDGHLKFTLRGEKLQGAWMLVRKGGRRGEEGERVWFLFKERD
                     EFARPGESITEEMPLSVTTGRDLDEIAAQSDRVWGPGGEVRQNGRKKTGKPATASSDR
                     RKAVRAPSADGIRARRRRAPKATAKANGREKPGAGRPSYQELLEHPGVQRATLPRSQS
                     VELATLVDAAPPGDEWLHEIKFDGYRMVCRIEKGKARFISRNGNDWTHKLPELAEAAG
                     GLAIRQAMLDGEVVSLKPDGTTGFQDLQNAFQAGRTGELVYYVFDVLHLDGRDVTGVP
                     LEARKEILKRAISQGTHKSIRYSDHLQGTGREIIDQACHLHLEGIISKRRNSLYRPGR
                     GLDWLKVKCLKRQEFVIGGFTKPSGSRSHFGALLLGYYDHGKKLIYAGRVGTGFDQRM
                     LATMKRKLTGLVRHTSPYADTSLINGQAADVSWVKPVLVAEVEFANWTNERLLRHATF
                     QGLREDKPATAVVHDEPLSLSDVKAMENGREAAAAQKDGKAVAFPGRRNVRKPTADAA
                     ANDEWAGVRLSHPDKILYPEHKLTKRDLANYYLQVADWMLTHVAGRPLAIVRCPGGSD
                     KPCFFQKHPGEGASKHLRQVNISENGAADFHASIDDASGLIALVQMGVLEIHAWGSRV
                     GRLEKPDRLIFDLDPDPSVDWPDVVNAARAVRLLLEELGLASFLKTTGGKGLHLVVPV
                     QPRAEWDEAKAFCRAVADFFVRAAPDRFIATMTKAARKGKIFIDYLRNGRGATAVAPY
                     STRAKAGATVSAPIAWEELTASLHSDHFTIENLPARLSKLKRDPWADMATTKQSITAA
                     MLERLAAR"
     gene            5240..5434
                     /locus_tag="VHS97_11855"
     CDS             5240..5434
                     /locus_tag="VHS97_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448944.1"
                     /translation="MAKRKSNDRELIDTGADKRFVRRDAQGQFKESDDVGRSLSQDVR
                     KQAKTKSKAGQGDKGDRPKT"
     gene            complement(5526..5789)
                     /locus_tag="VHS97_11860"
                     /pseudo
     CDS             complement(5526..5789)
                     /locus_tag="VHS97_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1117 a         1878 c         1780 g         1208 t
ORIGIN      
        1 cctctgccct tcggaaccga gaaagacgtt ttggaaccag tccccaggcc cgccagtcga
       61 tccttatccc tcagccgatg cggattacgg cggcatgtac ggcccgcgcg ccctagtaaa
      121 tgggttcacg aataaccctc cggcgggagc gatgatcttc ggccaatgta tcggtctggc
      181 ccagatcacc gacggcgcct cgcagacgat catcgtcggt gaggaccccg aggcgatcaa
      241 tgccatgtgg gtcagcggcc acaacatctt cgaccagtcg gccccgatca acgcccggcc
      301 gcccagcgaa ttcggtgagg aattgacgag tcaacacccc ggcggcgtca acgtcgtctt
      361 cgccgacggc tcggtccact tcctcaagaa ctcaaccgcc ctaccccccc tcgcggcgct
      421 ctgtacccgg gcgctcggcg aggtcctatc atccgacagc tattgaacga acgatcgaac
      481 cagctcccag gagacagaca tgaccttgcg ccaacctttc gtaacactcc tcggcctcgg
      541 cctcgccatc gccctggccg ccccgggcgc ccgggggggc gatatcgttg tcaacttcaa
      601 cgacctgagc taccccaacg ggtcgttcga cccgggagag ggcttccccg ctggcccggc
      661 ccccggcaca ggcagctacg acaacggctg ggatctcaac ggcggcttca cctcgaacgg
      721 aacgttcttc agcaacgcgt acgacaccac gtttaactcg tggagcggct gggcctattc
      781 gaacgtcaac gacccgacga cgaccggccc gtcccccttc ctcactgatt tcaaccacca
      841 gttcgccgcc atcaccggca ccgcccccgg cggatcgggg aattatggca tctcttccgg
      901 gagcggagcc tatatcaacc tcccctccgg cacgtcgcct gtctcattcg aagtgaccaa
      961 cacgacctac tcgttcctgt cgatgaccct tggcgacggc ttcggcaaga aattctccac
     1021 gggtgacttc ttcgaactga agattttcgg gtactcgggc ctgaacggca caggcacgca
     1081 agtcggcgag gttgacttct tcctggcgaa ctacacatcg gcgaattccc ttcccgtcga
     1141 cgtctggacc ctcgtcaacc tgacctcgct cgccgacgcg cagagccttg gcttcgatta
     1201 cgcctcgtcc gatgtcggcc agttcggcat caacacgccg gaattcttcg ccatggacga
     1261 ccttaccctg agcaccacct cagtcccgga gccttcaagt gtgattctcg ccctggccgg
     1321 gctaggaatc gtgggcctgt tcgcctcgca tcgccattgg cgatccgccg tggcccgctg
     1381 aaccgataac atcgcttcgg aattcccgct gtgctcctcg cgctcttttg aagtgaccgt
     1441 gaaaggtata cggcacgtcc gagtggcggc tatcctgaga ggtgatcggg gcgtggcgca
     1501 gcctggatta gcagccgcga aagtataagt tgagcaagta ggcgcggctg gcttaagtgg
     1561 cacgggcatc ttgcccgtcg tattttcaga cacgggctgg aagcccgttc cactacgggg
     1621 gccgaaatac gctcaattta ttatttcgcg gcgccttagc gcgctcgggc cacgacggca
     1681 cctcgaatgt tgtgtacgac gaagctggaa ctatctattg ttatgataaa gtgagcgatc
     1741 ctcccgtacg acacaaaatg gcctacgtcg gtcatgagcc ttagcgtgag actcttaagt
     1801 gccgctgtcc ggctaagcat gaagactggg agtgtccgat tccaaatgac aacgcaaccg
     1861 cctccatctg ccgcatgggt tagcgggcgg cgaggcgctc cagcatcgcc gctgtgatcg
     1921 attgctttgt cgtcgccatg tccgcccacg ggtccctctt cagcttgctg agacgcgccg
     1981 gaaggttttc aatcgtgaag tgatcggaat gcaggctggc ggtcagctcc tcccaggcga
     2041 taggggcgct cacggtggcg cccgctttgg cccgcgtcga atagggggcg acggcggtgg
     2101 ctccgcggcc atttctcaaa tagtcgatga agatctttcc cttgcgggcc gccttggtca
     2161 tcgtcgcgat aaaccggtcg ggcgcggcac gcacgaaaaa atcggcaacg gcgcggcaaa
     2221 acgctttggc ttcgtcccac tcagcgcgag gctgaactgg cactaccaga tgcagtccct
     2281 tgccgccggt tgtcttaaga aaagaggcaa gccccaactc ttcaagcaag agacgcaccg
     2341 cgcgggcggc gttgacaacg tccggccaat cgacagaagg atcgggatcc aaatcgaaaa
     2401 tcaggcggtc tggcttctcg agccgtccga cgcgagatcc ccacgcgtgg atttcaagga
     2461 cgcccatctg gaccagggcg atcagccccg aggcgtcgtc gatcgacgcg tggaaatcgg
     2521 ccgcgccgtt ctccgagatg ttcacttgcc gcaaatgttt cgatgcccct tcgcccggat
     2581 gtttttggaa aaaacatggc ttgtcgctgc cgccggggca gcggacgatc gccagcggac
     2641 ggccggcgac atgggtcagc atccagtcgg cgacctgcaa atagtaattg gccaaatcac
     2701 gcttggtcag tttgtgttcg ggatagagga tcttgtcggg atgggagagg cggacgccgg
     2761 cccattcatc gttggccgcg gcatcagccg ttggcttacg gacattgcga cggccaggga
     2821 aagccactgc ttttccgtcc ttttgcgcgg ccgccgcctc gcgaccgttc tccatcgctt
     2881 ttacatcact gagcgatagc ggctcatcat ggacgactgc tgtggccggc ttgtcctcgc
     2941 gaagcccctg gaacgtcgca tgccgaagca ggcgctcatt ggtccagttc gcaaattcga
     3001 cctcggcgac aagcaccggc ttaacccagg agacgtcagc tgcctggccg ttaatgagcg
     3061 aggtatcggc gtatggtgag gtgtgccgta ccaggccggt caacttccgc ttcatcgttg
     3121 ccagcattct ttggtcgaaa cctgtgccca cgcgcccggc atagatgagt ttcttgccgt
     3181 ggtcgtaata acccagcaat aaggcgccaa aatgcgagcg gctgccactt ggtttggtaa
     3241 agcccccgat gacgaattcc tggcgtttca gacacttaac cttgagccaa tccaggccgc
     3301 ggcctggccg gtagagggag ttgcggcgct tggagatgat tccctcgaga tgcaggtggc
     3361 aggcctggtc gatgatctcg cggcctgtcc cctgtagatg gtcgctgtag cgtatggatt
     3421 tgtgggtacc ttgggaaatg gcgcgtttca ggatctcttt gcgcgcctcg agcgggacgc
     3481 ccgtaacgtc tcgcccgtcg agatgaagga catcgaatac gtaatagacg agctcgccgg
     3541 ttcgacccgc ctggaaggcg ttctgtagat cctgaaagcc ggtcgtgccg tcgggtttga
     3601 gagagacgac ttcgccgtct aacatcgcct gccggattgc cagcccgccg gcggcctcgg
     3661 cgagctccgg aagcttgtgg gtccaatcgt tgccgttgcg gctgatgaag cgcgcctttc
     3721 ccttctcgat ccggcagacc atgcggtagc cgtcgaattt gatctcgtgc agccattcgt
     3781 cccctggcgg cgcggcgtcg accagcgtcg ccagctcgac gctttgcgac cgcggcagcg
     3841 tggcgcgttg cacgccggga tgttcgagca gctcctgata ggacggccgg ccggcgccgg
     3901 gtttttcccg gccgttggcc ttcgccgtcg ctttgggtgc gcgccgccgc ctggctctga
     3961 tgccgtccgc cgagggcgcg cgcacggcct tccgccggtc agatgaggcg gtcgcaggct
     4021 tcccggtttt cttgcggccg ttttgcctga cctcgccgcc gggtccccac acacggtctg
     4081 attgcgccgc gatttcgtcc agatcgcggc cggtcgtgac gctgagcggc atttcctcgg
     4141 tgatcgattc ccccggccgg gcgaattcgt cgcgctcttt gaacaagaac cagacgcgct
     4201 ccccctcctc gcccctgcgg cctcccttac gcaccagcat ccaggcgcct tggagctttt
     4261 cgccgcgcag ggtgaatttc agatgtccgt ccttcagccc cttagccgcg tcgccgatcg
     4321 gctcccagac gcccttgtcc cagagcatca cggtcccgcc gccatattcg ccctcgggga
     4381 tgattccctc gaaaccgccg tattccagcg ggtggtcttc gacctgcatc gccaggcgct
     4441 tcttggccgg gtccggatcg ggacccttcg gcacggccca gctcttcagc acgccgttca
     4501 gctccaggcg gaagtcgtag tgcagatgcg acgcgtcatg cttctggatg atataagcca
     4561 gttggccatg cggccggccg gcgcgcttgc cgcgcggttc tggcgttttg tgaaagtcgc
     4621 gtttgcgccg atattcgtcc aggcccatgc gaagacatcc tcaagtgaaa acgcggcccg
     4681 gctgcgctgg ccgtgcgttt gcgaaacgtg attcaactgc cccgacagcc agcggcgcct
     4741 gtgacagttt gaaagcccag tgttgatgcc gttgcttcgt gatccgtaaa tggcgaggcc
     4801 ggccgtttgt ctcaatcgcc gaataatggt gcaattgccg cgccaacgat ttggcatgtg
     4861 tgaataacgg cgacacgtgg ccagaatcag ccaaccgtgg attgccgcaa agaaagcagt
     4921 gtgcgaccga tcgcctcgct gctgcgccgc gaccggtcgg ttgataccgt ccagaaagcg
     4981 ctgatcgatt ttccgctaag tctacgaggg gtcggtaaga cgcggccaag tggcgctcag
     5041 ttccggcaga gcttcggacc ccgggtattg ggtctgcgat tagcgcggca acagtactga
     5101 tcgccaaagc ctccgcccag actccgagct tacataagtt ctgcgcggac cgcggattac
     5161 ggaaagcttg gcatgcgcgt tgcttatcga aagccgtgga agacacattt ttctggtcca
     5221 atcagcgggg agtaacatcg tggccaagcg aaaatcgaac gaccgtgaac ttatagacac
     5281 gggtgcggat aagcggtttg tgcggcggga cgcgcagggc caattcaagg aaagcgatga
     5341 cgtcggtcgt tccctgtcgc aggacgtgcg gaaacaagca aagacgaaat cgaaagccgg
     5401 ccagggggat aaaggcgacc ggccaaagac ctagtgccga gccgcaatgg atttagcggt
     5461 ccagttcgag gcgccggaag gcctgcgaag ggtacccgct ccggacgtcg acgcgccacg
     5521 ctgattcacg cgatggtcga atcggtatct ttgtctttca tccgatagct gtctgcttga
     5581 atcaccagcg cctccccgtg atgcatcggt cggtcgatca aggccgtgac cagcgtgttg
     5641 tccacgtcga acggggcgcc cgattcccgg aagattcgat tcgtggtaag cacgatcgat
     5701 ccggtttcgt aacgggtcgc gaccacctgg aacaacagat cggcaccgcg cttgtcgatc
     5761 ggcagatggc gcagctcatc gaggaggaca acggtgcgtc gatcgctact ctcactcgtt
     5821 tttcacgagg tttcaagata gcctagcgca tccagcaaga gaaggagggg ataggtcgtc
     5881 atcacacgga agaacccaga taattcattc cgtcgccttt ttgtcgtcca cgtagttata
     5941 ttcgacgttg gctcggggcg acccggccag cgaaagctcg aac
//
LOCUS       DATAQL010000678         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2970738, whole genome shotgun sequence.
ACCESSION   DATAQL010000678 DATAQL010000000
VERSION     DATAQL010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2970738"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1958
                     /locus_tag="VHS97_11865"
     CDS             360..1958
                     /locus_tag="VHS97_11865"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX3448945.1"
                     /translation="MSALHAKVDRLERENLELRQQVGYWKSRHRDALDRVSASEQKVE
                     QLEGEKRLLQADLFGRRSEARPGNDRSHDLDDPQDDSRTPKRNRGQQPGNPGPKRRDY
                     SHLPVREQFVELPPEQSVCPCCGLPLRRLSDTEDSEQIEIEVSAYRRVIHRRRYRRTC
                     TCDGPITLTAPPPPKLISKGRYGISVWVEILLDKYFSYRPTERLLASLRLWDLDLAPG
                     TVTDGLERLEVLLRPIDEALKARNSQGELHQGDETRWRVFIALEGKEGYGWWLWVVLG
                     PDTVIYLLDASRSHTVPEDHFRAESRGVLVVDRYAAYKAMRWVKDGVLVLAFCWAHVR
                     RDFIRVGKGWLELKTWALGWLRRIRALYRWNDRRLAAPKDTAAFGEADGRLRQAVAEM
                     KTEMETELARADLAAPCRKALESLHEHWKGMTRFVDDPRIPMDNNASERRARGPAVAR
                     KNFYGSGSLGSGRLAAALFSIYATLSLWKLNARRWLTWYFECCAVAGGKVPQDIQPFL
                     PWNLDAGKRSELGERSPAQGDDTS"
BASE COUNT          405 a          680 c          750 g          419 t
ORIGIN      
        1 cgggcggggt aatccgccgc atgagccggg cgggacgaca gtaagatgaa cagagcaaat
       61 aagatgcgtc tcatgcggcg taaagcttcg cttaacccgt ccctggttct cgcagttccc
      121 agaggaggtg gttaaactcg gccaccatgg atgatggctc ggcctccatt ttttgcttca
      181 ctcccctccg agtcacggtc tcggaatggg accgactccg cgccgcgccg ccttctcctc
      241 ctgagtcgtc actcggttcc gtctgctcgg ctccggccat tgctcaaggc ccctgtccag
      301 ttggcccgag cgactcctcc acagtgctga tcgctctggt ggaggacttg cgccgacaga
      361 tgagcgcgtt acacgccaaa gtcgatcgct tggagcgcga gaacctcgaa ctccgtcagc
      421 aggtcggata ttggaagtcg cgtcatcgcg atgccctcga ccgcgtctcc gcatcggagc
      481 agaaggtcga acaactcgaa ggcgagaagc gcctactcca agccgacctg ttcgggcgac
      541 gctccgaagc ccgacccggg aacgatcgct cccatgacct ggatgatcct caggacgact
      601 cccggacccc caagcggaac cggggccagc aaccggggaa tccgggtccc aagcgccggg
      661 attactccca tttgcccgtc cgtgagcaat tcgtcgaact gcctcccgag caatcggtct
      721 gcccctgctg tggcctgccc ttgcggcgac tcagcgacac ggaagactcg gagcaaatcg
      781 agatcgaggt gtcggcctac cgccgggtga ttcatcggcg gcggtaccgg cggacctgca
      841 cctgcgacgg cccgatcacc ctgaccgccc cgccccctcc caagctgatt tccaaaggtc
      901 gctatggcat ctcggtgtgg gtcgagatcc tcctggacaa gtatttcagc tatcggccca
      961 cggagcgctt gctggcctcg ttgcggctgt gggatttgga cctggcaccg ggcaccgtca
     1021 ccgacggctt ggagcggctt gaagtcctgc tgcggccgat cgacgaggcg ctgaaggcgc
     1081 ggaactccca gggggagttg caccaaggcg atgagacgcg ctggcgagtg ttcatcgctc
     1141 tggaaggcaa ggaagggtac ggttggtggc tgtgggtcgt gttgggcccg gacacagtga
     1201 tctatctgct ggacgccagt cgcagtcaca cagttcccga ggaccacttt cgcgccgagt
     1261 cgcgtggggt gctggtcgtc gatcgctatg cggcctacaa ggcgatgcgc tgggtcaagg
     1321 acggggtctt ggtcctggcc ttttgctggg ctcatgtccg gcgggatttc atccgggtgg
     1381 gtaagggttg gctggagttg aagacgtggg ccttgggatg gctgcggcgg attcgcgcgc
     1441 tctaccgatg gaatgaccgc cgcttggcgg ccccgaaaga taccgcggcg ttcggggaag
     1501 cggatggccg cctgcgccag gcggtggccg agatgaagac ggagatggag accgaacttg
     1561 cccgggcgga tctggccgcg ccgtgccgga aggccttgga gagcctgcac gagcattgga
     1621 aaggcatgac ccggttcgtg gacgaccccc ggatcccgat ggacaataac gcctcggaac
     1681 gacgtgcgcg cggtccggcg gtggcgcgga agaacttcta cggctcgggt tccctcggga
     1741 gcggccggtt agcggccgcg ttgttctcga tctatgccac gttgtcgctc tggaagctca
     1801 atgctcggag atggctgacg tggtacttcg agtgctgcgc agtggccggg ggcaaggttc
     1861 cgcaggatat ccaaccgttc ttgccgtgga acctcgatgc cgggaaaagg agcgaactgg
     1921 gggagcgtag ccctgctcag ggggacgaca cgtcctagat cgacagaacc ccgaatctcg
     1981 acgaccggaa ttcaatcgcg atgtgagggt gccagttcca cgcggtggaa cgctggacta
     2041 cgtactgctg cgccctgaac aaacgggttg ggcggtcgtt tacgaacgcg gacggcctgc
     2101 tcagtcgagc acgacgcggc ggcgcggccg cgaatcatgg cagcacttcc gtgtcctaaa
     2161 acattccggg attgcgagat gcggaacggg agccgattga cgacctcgct cgagcgtttt
     2221 catgattgag cgatccgtgc aagctcgatt gatc
//
LOCUS       DATAQL010000679         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1291439, whole genome shotgun sequence.
ACCESSION   DATAQL010000679 DATAQL010000000
VERSION     DATAQL010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291439"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VHS97_11870"
     CDS             complement(<1..228)
                     /locus_tag="VHS97_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448946.1"
                     /translation="MSNPRLSADRLNFSASDKSSAVIAVYYGEVGRRLFLREFIDSNP
                     TRSIKVMTSRRMALAGILGSAVVALALLTMGS"
     gene            290..1213
                     /locus_tag="VHS97_11875"
     CDS             290..1213
                     /locus_tag="VHS97_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3448947.1"
                     /translation="MRCSLAFVVGLSLFGPGAAWADEPPTYPLWPSGAPGAAGSDAGD
                     ENHSGDIPTITVYLPPKEKATGASILICPGGGYGFLATEHEGKEVARWLNSLGVAGVM
                     LKYRLGPKYKHPAPLDDARQAIRMVRWKSNEWGLDPGRVGVMGFSAGGHLASTLATHF
                     REAHPAPDDAINGFTERPDLAILVYPVVSFTADFSHKGSAKNLLGPDATKEQLAELSN
                     ETQVTEKTPPTFLAHTLADTGVPMENSVAFAMALRKHKVPFELHVFEKGQHGLGLGTG
                     WKGRIAPEPSFEAWPGLCATWLKSRGFLDKR"
     gene            complement(1764..3782)
                     /locus_tag="VHS97_11880"
     CDS             complement(1764..3782)
                     /locus_tag="VHS97_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3448948.1"
                     /translation="MNPADRHFEISRSLFREANDAFFLFDPQTQMIVDLNPAALRLTG
                     LEKDAACTMRLEELFAGSTPGGLDALTQALARTGFFHSREGYFLQRPSKGPLPVNLSV
                     SRIHTEPEPVGLIVARDVSDRKKAEEALKQIETRYNGLIASTGVMVWEIDAAGIIESI
                     SPAFETITGWSRRDWIGRPLGELLQPDDRERALLMHQRAWRGETLPRYELRIRTKAAD
                     WLDCEFLLVTKIRDGATERILGIARDITERRRTEMALEQAALMRRAKEDAERANRAKS
                     EFLSSVSHELRTPLTAILGFADVLDEHPYLRAGPPEIGEYLGTVVQNGQFLLALIDDL
                     LDISRIEAGQLRIEREPCFPARIVADAVESLRTKAEAKQLRIDVEVAGTLPPVIASDR
                     LRLQQILVNLLDNAIKFSQHGAVRLTVGMIVQPGGQPVLQFAVRDNGIGMAEAEMSEL
                     FQPFYRVRSGAQDSPAGTGLGLAICKRIAKRLGGDITVESDPESGSTFTLSIPAAEPR
                     DLDEFPPTGDLGEQTSSVLPSASSSRLAARILVTDDNLANQRLICLRLGRAGAEVVTA
                     ANGEEALDRASEAALEGRPFDAVIMDMQMPVLDGYEAVPRLRARGFTNPILAVTAYAM
                     SEDRDECLRLGCDDFISKPIDWDRLMSKLTRLLAAKNGASGHRSSSRD"
     gene            complement(3877..>4239)
                     /locus_tag="VHS97_11885"
     CDS             complement(3877..>4239)
                     /locus_tag="VHS97_11885"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX3448949.1"
                     /translation="NPLPAVPDRLQVAPKKPAQPPAPSEQSADFKRLVELRAYMIWIR
                     RGRPMGPAGDAVKEENWLEAERQITREVKARADVMWDQQGRPSGPAGEAVCEKNMRTA
                     AAALLKETEEELRRHPVD"
BASE COUNT          792 a         1304 c         1315 g          828 t
ORIGIN      
        1 actgcccatc gtcagcaggg cgagggcgac gacggccgat ccgaggatgc ccgccagcgc
       61 cattcgtctg gatgtcatga ccttgatgct ccgagttggg ttggagtcta taaactcgcg
      121 tagaaaaagc cgcctaccta cctcgccgta atacaccgct atgaccgcgc tggatttatc
      181 cgaggcggaa aaattgagac gatccgcgga caggcggggg tttgacacgg ccgtgcctgc
      241 ccgggagact gaagtggttg accatcactc cagttcggga gtttccacca tgcgctgttc
      301 gctcgccttc gttgtcggcc tttcgctgtt tggcccgggt gcggcctggg ccgacgaacc
      361 gccgacgtat cccctctggc ccagtggtgc gcccggcgcg gccggctccg atgccggcga
      421 cgaaaaccat tcgggcgaca tcccgacgat caccgtctac ctcccgccca aggagaaggc
      481 gaccggggca tcgatcttga tatgtccggg aggtggatat gggttcctcg ccaccgagca
      541 cgagggcaaa gaggtcgccc gatggctcaa ctcgctcggc gtggcgggcg tcatgctcaa
      601 gtaccggctc gggcccaagt acaagcaccc tgccccgctc gacgacgcgc ggcaggccat
      661 ccgcatggtg cgctggaaat cgaacgagtg gggtctcgac cccggccgcg tgggcgtgat
      721 ggggttttcg gccggcgggc atctggcttc gactctcgcc acccatttcc gcgaggcgca
      781 tcccgcgccg gacgacgcga tcaacggctt caccgagcgg cccgacctcg cgatcctggt
      841 ctaccctgtg gtgagcttca cagccgactt cagccataag ggctcggcca aaaacctgct
      901 ggggcccgac gccaccaagg aacagttggc cgagctatcc aatgagactc aggtcaccga
      961 aaagacacct cccacgttcc tggctcacac gctggccgac actggcgtgc cgatggagaa
     1021 cagcgtggcc tttgcgatgg cgctgcgcaa gcacaaagtg ccgttcgagc tgcacgtgtt
     1081 tgaaaagggg caacacggcc tggggctggg cacgggttgg aagggccgta ttgcgcctga
     1141 gccgtcgttc gaggcctggc caggcctctg cgcgacatgg ttgaagtcac gcggattctt
     1201 ggacaagcgc taggctgaac gcgacaatct ttggcgtgta aacacgcgag cggacgcggc
     1261 ccgcgccgtg accgtcgaga tgccgtgcgc caggccacga ccatcccgaa cgacggggtt
     1321 tgcaagaacg ctggcttagg tttcggcgaa cttggcacca cgtgcggcaa gcaactgtct
     1381 caaaatcgat ctgtggcatc gattctcgtc ttcgcagtaa cacccaatcg aaaaatccgt
     1441 tctatgagaa agggcggcca aaagatccag aactttcccg gcgtcaggtt cattcatttc
     1501 agatcgaaac ttccgtgcga agttagacca ggatttttca ttttccgccg acatggcttc
     1561 tttcacgagc tcttggctgg gtgagagatt gggcaaccaa acatcgtaat aatctcgctt
     1621 cgcatagtcg gacttcggaa ctcctctggg tggtctgcga accgtgccga gacgcaaccc
     1681 ctcatttagt tttctcgggg aaccaagtct taagacagtg atcgccatcg ggcttcaccc
     1741 ctaacgagcg attcggcgac gtgtcaatcg cggctgctgg agcgatggcc cgacgcgccg
     1801 tttttcgccg ccaaaagccg ggttagcttt gacatcaatc gatcccagtc gatcggttta
     1861 ctgataaagt cgtcgcagcc caagcgaagg cattcgtccc tgtcctcgct cattgcgtac
     1921 gccgtgacgg cgaggatggg attggtgaag ccgcgggcgc ggagccgcgg cacagcctca
     1981 tagccatcga gaaccggcat ttgcatatcc atgatcaccg cgtcgaacgg gcggccctcc
     2041 agggcggctt cgctggctcg atcgagagct tcctcgccgt tggcagccgt caccacttcg
     2101 gcgccggcgc ggccgagtcg gaggcatatc aagcgttgat tggccaggtt gtcgtcggta
     2161 accagaatac gggcggcgag ccgtgacgaa gaagccgagg gcaagactga ggacgtttgt
     2221 tcgcccaggt ctcccgtggg tgggaactcg tcgagatcgc gtggctctgc cgcgggaatc
     2281 gacagcgtga aagtcgagcc cgactccggg tcgctctcga ctgtgatatc cccgcccaag
     2341 cgtttggcga ttcgtttgca gatggccaat cccaggccgg ttccggcggg gctatcctga
     2401 gcccccgacc gaacccggta gaaaggctgg aacaattcgc tcatttctgc ctccgccatg
     2461 ccgatgccgt tgtcgcgcac ggcgaactgg agaaccggtt gcccgccggg ctggacgatc
     2521 attccgacgg tcaggcgcac cgcaccgtgc tggctgaact tgatcgcatt gtccagcagg
     2581 ttcaccagaa tctgctggag ccgcagtcga tcgctcgcga tcaccggggg aagcgttccg
     2641 gcaacctcga cgtcaattcg cagttgcttg gcctccgcct tggtgcgcag ggattcgacg
     2701 gcgtcggcga caatccgcgc gggaaaacat ggctcgcgtt cgatacgaag ctgtccggct
     2761 tcaatccggg agatgtccag cagatcgtcg atgagcgcga ggagaaactg accgttctga
     2821 acgacggtgc cgagatactc tccgatctcg ggcgggccgg cgcgcagata ggggtgttca
     2881 tcgagcacgt cggcaaaacc caggatggca gtcaaaggcg tgcgaagttc atggctcaca
     2941 ctcgagagaa actcactctt ggcgcggttg gcacgttcgg cgtcctcctt ggcgcggcgc
     3001 atcaaggcag cctgctccag ggccatttcg gtccgtcggc gttcggtaat gtcgcgcgcg
     3061 atgcccagga tccgctccgt ggcaccatcc cggatcttgg tcactagcag gaattcgcaa
     3121 tccagccaat ccgccgcctt ggtgcgaatc cgcaattcgt agcgcgggag tgtttcgccc
     3181 cgccaggcac gctgatgcat caagagcgcc ctctcgcgat cgtcgggctg gagcaactcg
     3241 cccagcgggc gtccgatcca atcgcgacgc gaccagccgg tgatcgtctc gaacgccgga
     3301 ctgatcgact cgatgatacc ggctgcgtcg atctcccaga ccatcacccc cgtcgaggcg
     3361 atcaagccgt tgtagcgcgt ctcgatctgt ttgagggctt cttcggcctt tttccggtcg
     3421 ctgacatcgc gcgcgacaat caatcccact ggctcgggct cggtatggat gcggctcacg
     3481 ctcaggttga ctggtaatgg accctttgat ggccgttgca ggaaataccc ttcgcgggaa
     3541 tgaaaaaagc ctgtccgggc cagagcctgg gtcaacgcat ccagaccgcc gggcgtcgat
     3601 cctgcgaaga gctcttccaa tcgcatcgtg caagccgcat ctttctcgag accggtcagt
     3661 cgcaaggcgg ccggattgag atcgacgatc atctgggttt ggggatcgaa caggaaaaaa
     3721 gcgtcgttgg cctcgcgaaa gaggctgcga ctgatctcga agtggcgatc tgccggattc
     3781 atgagatgtc gtcgggcctc ggttcgcagt ttcgtcgacg agtcagagga gcgtgtacgg
     3841 atgactcaga gaatcgtgta aagatgagac cgaaccctag tcaacggggt gtcggcgcag
     3901 ttcttcttcg gtttctttca gcaatgcagc cgcggcggtg cgcatattct tctcgcagac
     3961 ggcctcgccg gccggtccag acggacgccc ttgctggtcc cacatcacgt cagcgcgggc
     4021 cttgacttca cgggtgatct ggcgttccgc ttcgagccag ttttcctcct tgaccgcgtc
     4081 gcccgccgga cccatgggac gtccgcgcct gatccagatc atgtaggcgc gtagctcgac
     4141 cagccgtttg aagtcagccg actgttcgga cggtgccggc ggctgcgccg gtttttttgg
     4201 ggccacctgc agccggtccg gaaccgcggg cagcgggtt
//
LOCUS       DATAQL010000680         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_374868, whole genome shotgun sequence.
ACCESSION   DATAQL010000680 DATAQL010000000
VERSION     DATAQL010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374868"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1755
                     /locus_tag="VHS97_11890"
     CDS             91..1755
                     /locus_tag="VHS97_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX3448950.1"
                     /translation="MKRREKSGSLVPVLYGAALVLAYSGSAVAQQEPAVLSGVQYLKS
                     HYGTRPPGEMAMIALGLVKAEVPHSDPALQACVASIRSRFAGSDAYEPQLSNGSGTYE
                     AAAAIMALANFDPVENLTAITAIAAFIKGRQNANGSWDYNGRAQGDTSISQYAVLGLW
                     EAENAGVDIAPSTWDHAAEWYMSVQSSGGSWNYHRDEGSARPETVSMTAAGVGSLLIC
                     QRQLERYRQSRRGTSSLLTALGAESSQVDYRPTTTNAQIEQAIKRGIAWIGSNFVLNN
                     RNIVGTSPYYGLYGIERVGALGERQTIGRLDWYAKGRDFIVSTQRPDGSWNGDFGAEM
                     NTVWAMLFLTKSTAKTIRKINIQRLGAGTLLGGRELPKDLSSMTVAGGRVVSRPMNGA
                     IEGMLAVLDDPRAEQADAAVSGIVERYYAQGPEALRPHKIRFRKMLADRDHGIRRVAA
                     WALARTGDLDVVPPLIDAMVVPNEEEEVVAAIRMGLELLSRKIKGLGPPSPSTPDERI
                     AAARKWREWYQAIRPLDLEGQDEDAGIAGGANPPATAGTPTGSTPK"
     gene            1752..2705
                     /locus_tag="VHS97_11895"
     CDS             1752..2705
                     /locus_tag="VHS97_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448951.1"
                     /translation="MSQAVEVKPQAEVIPPEDELKVLGESTYDRVTSLLMAVVLGAIL
                     VVGWLALVYASNQAYASRVTSPLQIIEVGGGGGSPDGTPGSTEKVDVAGADAATAASN
                     NQEEAGDFEEPSVEQTPGAMMDTVSEASQSLAEVDLGATMPTGGPVAGGKRASKLGTG
                     GPGLGFGPGDGGVAREQRWSIVYTPGQTIDQYARQLDALGVEMAVVASAEQLLYISHF
                     SNPTPTKRHGTGRSDHRLYFLWQGRGRKPSDIELFKRAGVEIGDENVFQFYSPEAERR
                     LAELEVRYKGRSPGEIRITRFQVVAKGSSYDFAVVAQETLR"
     gene            3142..>5694
                     /locus_tag="VHS97_11900"
     CDS             3142..>5694
                     /locus_tag="VHS97_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3448952.1"
                     /translation="MATLEVHDAAGRVQFIELARDHPVLFGTSAACDVILEGDGIRPV
                     HGRIRWKKSRYRVEASPDAEFVQLNGTKMAAGSLHEGDEIEVGSSRIFLLRNDDTIDV
                     RQSTSAFDYGQTRVQAAPVVPVAPKNDTGGGPRPVLPRLKAPEAPAVAKDIGPGNLKG
                     RTRKRAGGAESDDFVGPDVAVGAQPKGFRGLIEWLTRVTGGHQAPGRERVLSSPLVIG
                     LVVALALLVGMGFWLKAIIASTVATQTFNLGVQHFDDGDYRTAIRDFDSFVASNPDEG
                     RTGKARVLRAMANVRQYISTEGATWSSALEASREMLEQVGDVEEFRDVRVDLAELLIK
                     VGEGLADRARLTEDAKALAEAESAVPLHARVAGEPARAFLDRSRLPAKLSEARAAVRK
                     AQIRTDAFAAMDKAISEGSATRVYEVRDNLVDQYADLAHSKDLVARMTAANELIRRAV
                     VVDTKRRPAERDPRPEPLGPPLSLVMRSNRNPAPNDPATDAIVFALTDGFGYAIDGGS
                     GAPLWHIPLGLASTFVPQAVAGEASVLAFDARHNELLKLDARKGALRWRLGLGEPVGD
                     PPLVLGNQLVQVLPSGKLVLIALESGELQSTVNLGRPLARSPEHDESGEHLYVLGRQD
                     CLFVLKRDPLSCIAVEYLGHVDGSIPCAITRLGRYLVIPENNSLADSRWHVLVVDESG
                     AKERPVQELEVSGWTWSTPASAGSIVWAAGDKGGYEAFAVGDYGTPTPFRSVARVTAD
                     AVASGPAFALARSDRELWVASGHTGRFELDAERGKIELKASLAQPGAALAPVQSKGRH
                     VVMTFHDDDTAGVALWGIDSETGAIAWKTIVAAPWPTAPLGLTGSDDLAMICR"
BASE COUNT         1075 a         1634 c         1944 g         1041 t
ORIGIN      
        1 gggaggcgtt cgcaacagct agccggctgg ataaaataac gacagccccc atgtggggtg
       61 atcaaaaatc ctctcgatgc gatttcaacg atgaagcgac gcgaaaaaag cgggagtctg
      121 gtcccggtgt tgtacggagc cgctctggtc ctggcctatt ccgggtcggc tgtcgcccag
      181 caggagccgg cggtgctgtc tggcgtccag tacttgaaga gccattacgg cacccggccg
      241 ccgggcgaga tggcgatgat cgcgctcggg ctggtcaagg ccgaggtgcc gcactccgat
      301 ccggccttac aggcgtgcgt ggcgagcatt cgctcccgat tcgccggcag tgatgcgtac
      361 gagcctcaac tttccaacgg gtcaggcacg tacgaagccg ccgccgcgat catggcgctc
      421 gccaatttcg atcctgtcga gaatctgacg gcgatcacgg cgatcgcagc cttcattaaa
      481 ggccgtcaga acgccaacgg ctcgtgggat tacaacggtc gagctcaggg cgacacgtcg
      541 atctcgcagt acgccgtatt ggggttgtgg gaagccgaaa acgcgggggt cgacatcgcg
      601 ccttccacct gggatcacgc ggcggagtgg tacatgtcgg ttcagagttc gggagggagc
      661 tggaactatc accgcgatga agggtcggcg cggcccgaga cggtttcgat gacggccgcg
      721 ggcgttggca gcctcttgat ctgccagcgg cagctcgagc gctaccgtca gtccaggcgg
      781 ggaaccagtt cgctcttgac ggcgctcggt gccgagagtt cgcaggtgga ttatcgcccg
      841 acgaccacca acgctcagat cgaacaggcc atcaagcgtg gcatcgcgtg gatcgggtca
      901 aatttcgttt tgaataatcg gaatatcgtc ggtacgtcgc cttattacgg gctctacgga
      961 atcgagcggg tcggggcgct cggggaacgt cagacgatcg gccggcttga ctggtacgca
     1021 aaagggcggg acttcatcgt ttcgacccag cgccccgatg gctcctggaa tggcgatttc
     1081 ggggccgaga tgaacaccgt ttgggcgatg ctgtttttga ccaagtcgac ggccaagacg
     1141 atccgcaaga tcaacattca gaggttgggc gctggcacgc tgctgggagg gcgagagctt
     1201 cccaaggatt tatcgtcgat gaccgtggcc ggcgggcggg tcgtgagccg gcccatgaat
     1261 ggcgcgatcg aggggatgtt ggcggttctc gacgacccgc gcgccgagca agcggacgcg
     1321 gcggtctcgg gaattgtaga gcgttactac gcccaggggc cggaggcgct gcggccgcac
     1381 aagatccgat ttcgcaagat gttggctgac cgcgaccacg ggatccggcg ggtcgcggcc
     1441 tgggcgttgg cccgaaccgg cgacctcgat gtcgttccgc cattgatcga tgcgatggtg
     1501 gttcccaatg aggaagaaga ggttgtggcg gcgatccgga tggggctcga gcttttgagc
     1561 cgcaagatca aagggctcgg tccgccttcg ccgtcgacac ccgacgagcg aatcgcggcc
     1621 gcccgcaagt ggcgcgagtg gtatcaggcg atccggccgc tggacctcga gggacaggat
     1681 gaagatgccg gcatagccgg aggggccaat ccgccggcca ccgcaggcac acccacgggg
     1741 agcacaccca aatgagccag gcagtcgagg tcaaacccca ggccgaggtc atcccccccg
     1801 aggacgagct gaaggtgctc ggcgagtcga cctacgacag ggtcacgtcg ctgttgatgg
     1861 ccgtagtctt gggggcgatt ctggttgtcg gctggctggc cctggtctac gcttcgaatc
     1921 aggcttatgc gtcacgagtg acgtcgccac tgcagatcat cgaagtcggt ggcgggggag
     1981 gaagtcccga cggaacgcct ggctcgaccg aaaaagtgga cgtcgccggg gccgacgcgg
     2041 ctacggcggc ttcgaacaac caagaagagg cgggcgactt tgaagagcct tcggtcgagc
     2101 agacaccagg ggcgatgatg gacacggtgt cggaggccag ccagagcctg gcggaagtcg
     2161 atttgggggc gaccatgccc acgggtggtc cggttgccgg cggcaaacgc gcctccaagc
     2221 tgggcacggg cgggccgggg ctaggctttg ggcccggcga cggcggcgtc gcccgcgaac
     2281 agcgctggag catcgtctac acccccggtc agacgatcga tcagtatgcc cggcagcttg
     2341 acgccctggg ggtcgagatg gccgtcgtcg ccagcgcgga gcagctctta tacatttctc
     2401 atttttccaa tccgacgccc accaagcggc acggcaccgg gcggagcgat caccggctct
     2461 atttcctctg gcaagggcgg ggccgcaagc cgtcggatat cgagctcttc aagagggccg
     2521 gcgtcgagat cggcgatgaa aacgtctttc agttttattc accagaggcg gaacggcggt
     2581 tggcggagct cgaggtcagg tacaagggac gaagtcccgg cgagattcgc atcacgcggt
     2641 ttcaggtcgt ggccaaggga agttcttacg atttcgcggt ggtcgcgcaa gagacgctgc
     2701 gatagggcgc agtccgccct ggcgattgtc agacagcatc tcaaagaaca aagagcatct
     2761 caaagaggag taagattaag aggaagatta agactaggaa gatgattaag agcaggatca
     2821 agattaagag tagggcgtcg agtccgcgcc taacgaatgc tggtgcgatg atgaataact
     2881 agaaggaact cgacacgtga atctgacaaa ggcaaccggg cgaaatggat cctaatctta
     2941 ctcttgctca taatcctgtt cctaatctta atcttcatct taatcttaat ctttctctga
     3001 tgtgcgagcc gttcggcaat ccatcaactg aagattctcc gaccactttc atgacggccg
     3061 ctgttttcgg atatgcaccg gctcgacggc ggtggcggcg gagaatcgtc tgattcacaa
     3121 ggaaaaacga gtcgttctgg catggcaaca ctggaagtac atgacgccgc aggacgtgtt
     3181 cagttcatcg aactcgcgcg cgatcatccg gtcttgttcg ggacgagcgc ggcctgcgat
     3241 gtcatcctgg agggtgacgg aatccggccg gtgcacggcc ggattcgctg gaagaagagt
     3301 cgataccggg tcgaggcttc gccagatgcc gaatttgtcc agttgaacgg caccaagatg
     3361 gcggccggca gcctccacga gggagatgag atcgaggtcg gatcttcccg gatctttctg
     3421 ctccgcaacg acgacacgat cgacgttcga caatcgacga gcgcattcga ctacggacag
     3481 acgcgtgtac aggcggcgcc tgtcgtcccc gtggccccaa agaatgacac aggcggcggt
     3541 cctcgccctg ttctacctcg cctgaaagcg ccggaagcac cggctgtcgc caaggacatc
     3601 gggcctggga atctgaaagg gcggacgcgt aaacgcgcgg gcggtgccga gtcggacgac
     3661 tttgtcggcc ctgatgtcgc cgtcggcgca cagccaaagg gctttcgggg cttgatcgag
     3721 tggctcacgc gggtaaccgg cggtcaccag gcaccgggac gggaacgggt cctgtcgtcg
     3781 ccactcgtga tcgggctcgt cgtcgcgctc gcacttctgg tcggaatggg gttctggttg
     3841 aaggcgatca tcgcctcgac cgttgcgacg cagacattca atctcggtgt ccagcatttt
     3901 gacgatggcg actatcgcac ggcgatccgc gatttcgact cattcgtggc gagcaatccc
     3961 gacgaggggc gcacggggaa ggcacgcgtg ctgcgggcca tggcgaacgt gcgtcagtat
     4021 atttcgaccg agggagctac ctggtcgtcg gcgctggagg catcgcggga gatgctcgaa
     4081 caggtgggcg atgtcgaaga gtttcgcgat gtacgggtcg atttggccga gctgctgatc
     4141 aaggtcggcg aaggactcgc cgatcgcgcg cggctgacgg aagatgcgaa ggcgctggcc
     4201 gaagccgagt cggcggttcc gctgcacgcc cgggttgccg gcgagcccgc gcgtgcgttt
     4261 ctcgatcggt cgcgactacc ggccaagctt tcggaggcac gcgcggcggt tcgcaaggcg
     4321 cagatccgca ctgacgcctt tgcggcgatg gacaaggcga tcagcgaagg tagtgcgacg
     4381 cgcgtctatg aggtgcgcga caacctggtc gatcagtatg ccgacctggc tcattccaaa
     4441 gatctggtgg cgcgaatgac ggccgcaaat gagctgatcc gccgcgcggt cgtggttgac
     4501 acgaaacgcc ggccggccga gcgcgatcca cgtcccgagc cgctggggcc gcccttaagt
     4561 ctggtcatgc gatcgaaccg aaatcccgcg cccaacgacc cagcgaccga tgcgatcgtg
     4621 ttcgccctga cggacgggtt tggttatgcg atcgatggag ggtcgggagc ccccttgtgg
     4681 catattccgc ttggattggc ctcgacattc gtgcctcaag cggtcgcggg tgaggcgagc
     4741 gttctggcgt tcgacgctcg tcataatgag ctcctcaagc ttgatgcccg gaaaggcgcc
     4801 ctgcggtggc gtctgggact aggtgaaccg gtcggagatc cgccgctcgt actgggcaac
     4861 cagctcgtcc aggttttgcc gagcggcaag ctggtcttga tcgcgcttga gtcgggcgag
     4921 ctgcagtcga cggtcaacct gggccggccg ctggctcgct cgcccgagca cgacgaatcg
     4981 ggcgagcacc tctacgtgct gggccgccag gattgcctgt ttgtgctgaa gcgcgatccc
     5041 ctctcgtgta tcgccgtcga gtatctcggc cacgtggacg gctcgatccc gtgtgcgatc
     5101 actcgactgg gccggtacct ggtgatcccc gaaaacaact cactggcgga tagccggtgg
     5161 cacgtcttgg tcgtcgacga atccggcgcg aaggaacggc cggtgcagga gctcgaagtt
     5221 tccggttgga cgtggtccac gcccgcaagc gcaggctcga ttgtgtgggc tgccggtgac
     5281 aagggaggct acgaggcgtt tgccgtgggt gattacggga ccccgactcc gtttcggtcg
     5341 gtggcccggg ttacggcgga tgctgtggca tcggggccgg ccttcgcgct ggcgcgctcg
     5401 gatcgcgaac tctgggttgc gtcgggccat acgggccggt tcgagctcga tgccgagcgc
     5461 ggcaagatcg agctgaaggc atcgctggcg cagcccggag cagcactcgc gcccgttcaa
     5521 agcaagggcc ggcacgttgt catgacgttt catgacgatg atacggcggg cgtcgcgctc
     5581 tggggaatcg attcagagac cggcgcgatc gcttggaaga cgatcgtcgc ggccccttgg
     5641 ccgacggcac cgcttggttt gaccggctcg gacgacctgg cgatgatctg tcgc
//
LOCUS       DATAQL010000681         6573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1750642, whole genome shotgun sequence.
ACCESSION   DATAQL010000681 DATAQL010000000
VERSION     DATAQL010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750642"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VHS97_11905"
     CDS             complement(<1..447)
                     /locus_tag="VHS97_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448953.1"
                     /translation="MRPRRRRAVRPIIDHLDDRCLLSATTGFTPAQVTGAYGLDAITF
                     KSSSGSTVTGNGAGETIALIEMYHDPNLTSDLHTFDQKYGLPDPTLNVDNLAGTQTDS
                     GWALEESLDVEWAHAIAPGANILVVEAAPANSDLGAIQNLMAAVKAA"
     gene            complement(706..2553)
                     /locus_tag="VHS97_11910"
     CDS             complement(706..2553)
                     /locus_tag="VHS97_11910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3448954.1"
                     /translation="MAGDLSADSAEVDLDQARRIHALCEEFECRWRKGGRPRIEELIE
                     QAPNGDRPSLLEELLILELELRRAGGERPGLGEYRDRFPEGEGRVEAAFAQAAGASRA
                     SRKTMTVPSGAASRDTSCGSNTNSNFEVIGDYELLEEIARGGMGVVYKARQRGANRLV
                     ALKMILTGQMASPDERERFLREAELAANLDHTNIVPIYDVDEFQGCPFFSMKLIEGEN
                     LSRQIKAKRRAGLAHDPQTGAGLMATIARAVHYAHERGFLHCDLKPSNILIDREGRPY
                     VTDFGLAKRASEDSTVSISGAILGTPSYMAPEQASGSRKGLRPTTDVYGLGAIFYELL
                     TGRPPLTAETVPETVVAVLERDPVPPRVIRPGIPRELETICLKCLEKSPNDRYASAAA
                     LADELDRYRQGEVIDATTLIPRIRRWNRREPELVARLGGLLLIALIAQFNYEFLSADR
                     SFRLHYTIQGVLALWAVSAVIFQAMLRSGRHPDRVRVLWSAADIALLTVELKLFEQLQ
                     PAASGDPIVRVETTLLVGYPLLIAASGLWWRVNLVWITTALAMSAYSWLYIDAALRWH
                     DGWVSWSPSPDLQHSNVFLAGLLLTGYVVARQVRRILLLSRYYEHRPAA"
     gene            complement(2553..3221)
                     /locus_tag="VHS97_11915"
     CDS             complement(2553..3221)
                     /locus_tag="VHS97_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3448955.1"
                     /translation="MDWIPQPREGLITVDEGASITEWLGNLREGDQAAAQPLWERYFS
                     KLVTVARTKLRRMRRTTADEDEEDAALSAFNSFCAGAARGKFPQLADRDDLWRLLVVI
                     TARKAMAQANRQGRQKRGGGRVVEEAVLFGHGLGNADGSIAGLERIAADGPTPEFAAM
                     MAEECKRLLDALDDESLKQVAVSRMEGYSNDEIADQLGCARRTVARRLDLIRKTWLAA
                     EEVA"
     gene            3519..4547
                     /locus_tag="VHS97_11920"
     CDS             3519..4547
                     /locus_tag="VHS97_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX3448956.1"
                     /translation="MWGRLIGHGGGSKAIDRRSLLRRAIVGGTVFGSLDRIPGPALFA
                     DEPKRSPVEEAEGELERAQSLARVATRLPLVVARTDQYQGVGDVSESFLKLALGDCEN
                     IAEDYFDYYQRQGFDVKRPGRRLTLVVFRDERPYLEFARKFVRGLTQFDWGFYSKAEN
                     WLVLFDFRNVPASERGAAYKNVRVLAHEATHQLTFNSGLLNRKGDLPRAVVEGLASYS
                     ETRPLHGHGEPGKLNGALLDDLAHILRRAKWTTATDLLTDDNAAFGNTLDQRLLAYAQ
                     GWLLVYHLMKTPARLPQLQAYLKTIATRTNNDRRYDDAERHFGDLDRLDQELRREAIQ
                     LQISRQTR"
     gene            4589..>6573
                     /locus_tag="VHS97_11925"
     CDS             4589..>6573
                     /locus_tag="VHS97_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448957.1"
                     /translation="MCATSNTIEPVYSDAEREELGHLDYFVARLDDLCQRRLLTPEAC
                     ATVVAESRGRRAAIDLTGRYNGAIAHARKLAKSYPREALEWAELARGLDPSRIDAWKL
                     IVALNWDLGNDEDAVARCGEAAERFPEFQTELYRLKVEQARRGDERRHQAEKARQDAA
                     ATEWLALAKLALEEKRDADVIALCRQILAIRPDNVDTFVMMAYAQQRSGQLDAALESY
                     EILSRLQPFNKTWLQWIKSIQLRRGVARLTGTSVETTATAGAAGDRAVLHAGENAGPL
                     PISWSSFAAEFLEEHWQKLILCLAVLLIVVSSTVGAHLLLGPLLWSAVGKCTLAMVAT
                     LLFAAFGAGLIRWGADRAGRVMLVATLIVVPIHFMLVGEMKLLRDPSALNVAFLAMEG
                     AVLVAMVRWVSGMLAPAANARFLTAALLLLSLGTAATTRGSPTASGLQLASFELSPLV
                     FLGAVWILGARRWGATSEQHREFVYMALGLLGFALVACLARTGGYALRLDAALYALPA
                     MLIAVSCVHAARRLDPDEPDKERLAFLRLGGYALSGLAFALVLARPPTASSVYSANTL
                     AVSLLGLGLYAGSLKLDRHPAFLYLAVGAIVAGRMGAHYFLADRWHAIEEVVRRLLGY
                     PHVLPTPFRAILAVVPSTALAWLARWFPRHWDDRRLAR"
BASE COUNT         1210 a         2019 c         2038 g         1306 t
ORIGIN      
        1 cgctgctttc acggcagcca tcaagttctg aatggcaccc aagtcggaat tcgcaggtgc
       61 agcttcgacc accagtatgt tggcgcccgg cgcaatcgcg tgtgcccatt cgacgtcaag
      121 cgattcctct agtgcccagc cgctgtccgt ttgcgtacct gccaggttat cgacattgag
      181 cgtcgggtcg ggcagaccgt acttctgatc aaacgtgtgc aggtccgatg tcagattggg
      241 gtcgtggtac atttcgatga gcgcaatcgt ctcgcccgct ccgttgcccg tgacggtcga
      301 tccggaggac gacttgaacg tgatggcgtc caagccatag gccccggtga cctgcgcggg
      361 tgtaaagcca gtggtcgcgg acaacaagca acgatcgtcg agatgatcga taatgggccg
      421 aacggcacgg cggcggcgag gacgcatgat cagtcaatct ccaattgatc cgcaatccgt
      481 tacggcacca cgtcctcctc ggaaaccaga cgacacgacg taacttcagg tcgaatgcgg
      541 ttatcaaacc gtcgaatggg aacggggaat tcggcgtgaa cgagccaata tgcgctcaat
      601 gcgcagactc gtctgaatca agttcatcat tctaaacgca atccgttccc cgaaccgcgc
      661 gctcgcgtac gcattcacga gggttttcgc gtaaaaattt gtaaatcagg ctgccggccg
      721 atgttcgtag tatctgctca agagcaggat gcgcctgacc tgcctggcga cgacgtagcc
      781 tgtcaaaagc agccccgcca ggaagacatt ggaatgctgc aggtcggggc taggactcca
      841 agagacccag ccgtcatgcc atcgcagggc ggcatcgata tagagccagc tataggcgga
      901 catcgccagc gcggtcgtga tccaaaccaa gttgacgcgc caccacagtc ccgaggccgc
      961 gatcaagagc ggataaccca cgagcaatgt ggtttcgacc cgcacgatcg gatcgccgct
     1021 ggctgcgggc tggagctgct caaacagctt gagctcgacc gtgagcaggg cgatatcggc
     1081 ggccgaccag aggacacgta cccgatcggg atgccggccc gagcggagca tggcctggaa
     1141 aatgacggcc gagaccgccc agagggcgag aactccttga atggtgtaat ggagccggaa
     1201 actgcggtcg gccgataaga attcatagtt gaactgagcg atcagtgcga tcagcaacag
     1261 cccgccgagc cgcgccacca actctggctc gcgccggttc cagcggcgaa tccgggggat
     1321 gagcgtggtt gcgtcgatca cttcgccttg acggtagcga tcgagctcgt cggccagggc
     1381 agcggccgac gcgtagcggt cgttcggcga tttctccagg cacttcaggc agatggtttc
     1441 cagctccctg gggattccgg gtcgaatcac acgtggagga accgggtctc gctcaagcac
     1501 ggcgaccacg gtctctggca ccgtctcggc ggtcagcggc gggcggccgg tcaatagctc
     1561 ataaaagatc gcgcccaatc catagacgtc ggtcgtcggc cgcaggcctt tgcgcgaacc
     1621 actggcctgc tccggggcca tgtagctggg tgttcccaag atcgcgccgc tgatcgacac
     1681 cgtgctgtcc tcactggccc gcttggccag gccaaagtcg gtgacatagg gccttccctc
     1741 gcgatcgatc aagatattcg atggcttcaa gtcgcagtgc aggaagccgc gttcatgggc
     1801 gtaatggacg gcgcgagcga tcgtcgccat caggccggcg ccggtttgag ggtcgtgcgc
     1861 caggccagct cgcctcttag ccttgatctg gcgcgacaga ttctctcctt cgatgagctt
     1921 catgctgaag aatggacagc cttgaaattc gtcgacgtcg tagatcggta cgatgttagt
     1981 atggtcgagg ttggcggcca gctcggcctc gcggagaaac cgctcacgtt cgtctgggga
     2041 ggccatttga cccgtcaaaa tcatcttgag cgcaaccagc cggttcgcgc ctcgttgtcg
     2101 ggctttgtag acgaccccca tgccgccgcg ggcgatttct tcgagcagtt cgtagtcgcc
     2161 gatcacctcg aaattcgagt tcgtgttcga gccgcaggag gtgtcgcggg acgctgcacc
     2221 gctgggcacg gtcatggtct tgcgtgacgc gcgcgacgca cctgccgcct gggcaaaggc
     2281 cgcctcaacc cgcccttcgc cctcgggaaa gcgatcacgg tattcgccca gaccggggcg
     2341 ctcgccgccg gcgcggcgga gctcgagctc gaggatcaag agctcttcca ggagcgaggg
     2401 ccggtctcca tttggtgcct gctcgatgag ctcttcgatg cgcggccggc cgcccttgcg
     2461 ccagcgacat tcaaactctt cacaaagggc atgaatgcgg cgagcctgat ccaggtcgac
     2521 ctcagcactg tccgcggaaa gatcgccggc catcaggcga cctcctcggc ggccagccat
     2581 gtcttgcgaa tcaggtcgag tcgacgcgcc acagttcgcc gggcgcagcc aagctggtcg
     2641 gcgatctcgt cgttgctgta accttccatc cggctgacag cgacctgctt aagcgattcg
     2701 tcgtcgaggg catcgagcag tcgtttgcac tcttcggcca tcatggcggc gaactccgga
     2761 gtcgggccgt cagcagcgat tcgctcgagt ccagcgattg acccgtcggc attcccgagg
     2821 ccatggccga agagaaccgc ctcttcgacc acacggccgc cgccgcgctt ctgccggccc
     2881 tgccggttgg cctgtgccat ggccttgcgc gccgtaatca ccaccaagag ccgccaaaga
     2941 tcgtcgcggt ccgccagttg gggaaactta ccgcgcgcgg caccggcgca aaagctattg
     3001 aacgcgctca aggccgcgtc ttcttcgtct tcgtcggcgg tcgttcgccg catccgccgc
     3061 aacttggtgc gcgccactgt caccagcttg gaaaaatagc gctcccagag tggctgcgcg
     3121 gccgcctggt ctccttctct cagattgccc agccactccg tgatcgaggc accttcgtcc
     3181 acggttatca gcccctcgcg cggttgcggg atccaatcca tcgaactatt gtagccacaa
     3241 cgagatcgcg ttcccattca gaccagtcgt gaacgacaga aaccgtcgcg attcgaatcg
     3301 ccttttttgc attagagtgg caccccatcg gcgagattga tcattagtaa ctttaggaga
     3361 cggtcggttc catcggcctg gaaatcgcga ccgatgtcgc cggcccagac tgccctgcgt
     3421 tttctggagt gtcctctcgt gatggagcga tgggttatga tccataattg cgtgtcggcc
     3481 gaatcgatct aagagttagg caccgaggag ttccggacat gtgggggcga ctgatcggac
     3541 acggcggagg cagtaaagcc atagatcgac ggagcttgct acggcgggcg atcgttggcg
     3601 ggacagtgtt tggtagttta gaccgaatcc ccgggccggc actctttgcc gacgagccca
     3661 agcgctcgcc ggtcgaggaa gctgagggag agctcgagcg tgctcagtcg ctagcccgtg
     3721 ttgccacgcg cttgccgctc gtcgtcgcgc gcaccgacca gtatcaggga gtgggtgatg
     3781 tctccgaatc cttcctgaaa ctcgctctgg gtgactgcga aaacattgcc gaggattact
     3841 tcgactatta ccaacggcag ggttttgacg tcaaacgtcc cggtcgtcgc ctgaccctcg
     3901 tcgttttccg tgacgagcgc ccgtatctcg aattcgcccg aaaatttgtt cgcggcctga
     3961 cccagtttga ctggggcttt tattcaaaag ccgagaactg gctggtgctt ttcgattttc
     4021 gcaatgtgcc cgcgagcgag cgtggcgctg cttacaaaaa cgtgagggtt ctggcgcacg
     4081 aagcgacgca ccagttgacg ttcaattccg gattgctgaa tcgtaagggc gacctgcccc
     4141 gcgctgtcgt cgaggggctt gcctcctata gtgagacacg cccgctgcac ggtcacggcg
     4201 agccgggaaa gctcaacggg gcactgctcg atgacctggc ccacattctc cgccgagcga
     4261 aatggacgac cgcgaccgat cttttgaccg atgacaacgc ggctttcgga aataccctgg
     4321 atcaaaggtt gctggcgtac gcccagggat ggctgctcgt ttaccatctg atgaaaactc
     4381 ccgcccgctt gcctcaacta caggcctatc tgaagactat cgccacccgc acgaacaacg
     4441 atcgccggta cgacgacgcg gaacggcact ttggcgacct cgatcgactc gatcaagagc
     4501 tgcgccgcga ggcgattcag cttcaaataa gtcgtcaaac gagatgaatg ccgcagctct
     4561 gtcgcccgag taagaaagca gcgtgaccat gtgtgcgacg agcaatacga tcgaaccggt
     4621 gtattccgac gccgagcgcg aagagctggg acatctcgac tatttcgtcg cgcggctgga
     4681 tgacctatgc cagcgtcggc tgctgacacc ggaggcgtgc gcgacggtcg tcgccgagag
     4741 ccgtggccgg cgcgcggcga tcgacctgac cggccgatac aacggcgcga tcgcacatgc
     4801 ccgtaagctc gccaagagct acccccggga ggccctggaa tgggccgagc tggcacgcgg
     4861 gctcgatccc tcacggatcg acgcatggaa actgatcgtt gccctcaact gggacctggg
     4921 gaacgatgaa gatgcggtcg cccgctgtgg tgaggctgcg gaacgatttc cagagtttca
     4981 aactgagctc taccgtctca aggtcgagca agcgaggcgg ggcgacgaac ggcggcacca
     5041 ggcggaaaag gcgcgacaag acgccgcagc cacagaatgg ttagccctgg ccaaactggc
     5101 gctcgaggaa aagcgcgacg ccgacgtgat cgcgctatgt cgccagatcc tcgccattcg
     5161 ccctgataat gtcgacacgt ttgtgatgat ggcatatgcc cagcagcgca gcgggcagct
     5221 cgacgcggca ctcgaatcgt acgagattct gtcgcggttg caaccgttca acaagacctg
     5281 gttgcagtgg atcaagagca tacagcttcg caggggtgtg gcgcgattga ccggcaccag
     5341 tgtcgaaacc acggccacag cgggagcggc cggcgatcgt gccgttcttc atgcgggcga
     5401 aaacgccggt ccgctgccga tatcgtggtc aagcttcgcg gccgagttcc tcgaggaaca
     5461 ctggcaaaag ctgatccttt gcctggccgt tttgttgatc gtggtcagct cgaccgtcgg
     5521 cgcccacttg ctgctgggcc cgttgctctg gtcggctgtc ggcaagtgca cgctggcgat
     5581 ggtggcgaca ctgctgttcg cggcgttcgg agcggggctc atacgctggg gcgccgaccg
     5641 cgccggccgg gtgatgctgg tcgccacact catcgtcgtg ccaatccatt tcatgctggt
     5701 cggcgagatg aagctgttgc gcgacccttc cgccttgaac gtggcgtttc tggcgatgga
     5761 aggcgccgtc ctggtcgcta tggtgcgctg ggtgagcggc atgctggcgc cggctgccaa
     5821 cgcgcggttc ttgaccgcgg ccctcttgct cctctcgttg ggaaccgctg cgacaacacg
     5881 cggatcgccg acggcttcag gcttgcagct cgcctcgttt gaactctcgc cgctcgtttt
     5941 cctgggagcc gtgtggatcc tgggggctcg gcgctggggt gccacgagcg agcaacatcg
     6001 cgagttcgtg tacatggcgc tcgggctgct cggctttgcc ctggtcgctt gcttggcgcg
     6061 taccggaggc tatgcccttc gcctcgatgc cgctttgtat gctctcccgg ccatgttgat
     6121 cgcggtttca tgcgttcacg ctgcgagacg gctcgatcca gatgagcccg acaaagagcg
     6181 cctcgcgttc ctgcggctag gtggctacgc cctctctggt ctcgcttttg ctcttgtctt
     6241 ggccaggccg ccgactgcct cgtcggtata cagcgccaat accctggcgg tgtcattgct
     6301 cggattgggg ctctatgcgg gctcgctgaa gcttgatcgc catccggcat tcctgtactt
     6361 ggccgtcggc gcgatcgttg ccggccgcat gggtgcccac tatttcctgg ccgatcgctg
     6421 gcacgcaatt gaagaagtcg tccggcgatt gctgggttat ccccatgtgc ttcccactcc
     6481 attccgggcg atcctggctg ttgtccccag cacggctctt gcctggcttg ctcgttggtt
     6541 tccgcggcat tgggacgacc gcaggctggc gcg
//
LOCUS       DATAQL010000682         5743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2511882, whole genome shotgun sequence.
ACCESSION   DATAQL010000682 DATAQL010000000
VERSION     DATAQL010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2511882"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VHS97_11930"
     CDS             complement(<1..1177)
                     /locus_tag="VHS97_11930"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX3448958.1"
                     /translation="MRTIQIAGGRIIDPGQDFDQVGDLWISRGHILPLGAGYEEAEVV
                     IDAKGMIVCPGLIDVHVHLREPGNEEDETIATGASAALAGGVTSVACMPNTLPAIDTQ
                     AAAEFVVLQAQRARQANVYPVGAVSKGRKGEELAELGQLVAGGAVAFTDDGAPVASAA
                     LMRRALQYCKMFDRVIMQHCQVPELTTGGVMNEGFESMRLGLGGMPAAAEDIMVARDI
                     RLAEITGGRLHIQHISTARSVELVREGQRRGVRVTAEACPHHFTLTDERLRSFDSNFK
                     MNPLLRTWSDVEAVIGGLKDGTIEIIATDHAPHAREKKLREIDQAPFGIVGLETLIPI
                     AIASLIEPGHLSWIEALRKLTINPAQLLGIPKGTLRGGADADVTIIDPSERWTIDPTR
                     "
     gene            complement(1232..2224)
                     /locus_tag="VHS97_11935"
     CDS             complement(1232..2224)
                     /locus_tag="VHS97_11935"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX3448959.1"
                     /translation="MTSAPFAGPAESVAPWSRKHLLGLEELSRDEIVAILDTAESFAE
                     ISTRSRKKVPALQGRIVFNLFFENSTRTRTSFSLAAKRLSADTQDFSPSGSSLSKGES
                     FIDTARNIEAMGADVVVIRHPTPGTPHMLAEHLKCSIINAGDGAHEHPTQGLLDLMTI
                     RRAKGKFDGLTVALLGDIAHSRVARSNIWGLLKLGAKVILCGPPTLVPKGWERLGCEV
                     AYHLDDVLPRCDVINVLRIQFERQQGGMFPSVAEYSHFYMMTKERLGLAREDLLLLAP
                     GPINRGVELTPEVADGKHSAILDQVTNGLAVRMAVLYLLSGRAGAKGLPLDRRN"
     gene            complement(2221..2799)
                     /gene="pyrR"
                     /locus_tag="VHS97_11940"
     CDS             complement(2221..2799)
                     /gene="pyrR"
                     /locus_tag="VHS97_11940"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX3448960.1"
                     /translation="MNPGEQRLCDGHEIGGLISSMARKLADARRVGVPLYLVGVRTRG
                     VPLAQRLAQELCRLLGQDVTVGAVDITLYRDDIGRTQRWPVLRGTDIPFDLDGAEVVL
                     VDDVLFTGRTVRAALNAICDLGRPACVRLAVLVDRGHREIPIQPDVVGLHMVTNFDDH
                     VGVRLQPVDPVEEVVKMAAGSRKSPLPRSAEP"
     gene            2994..4007
                     /gene="ispH"
                     /locus_tag="VHS97_11945"
                     /gene_synonym="lytB"
     CDS             2994..4007
                     /gene="ispH"
                     /locus_tag="VHS97_11945"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX3448961.1"
                     /translation="MKIILANPRGFCAGVNMAIECLERALDFFGPPLYVYHEIVHNKF
                     VVERFRDRGTVFVESLDQVPPGAPLLYSAHGVSPQIREQARIRQLVAIDATCPLVIKV
                     HLEAVRYAREGYTIVLIGHEGHDEVIGTMGEAPEQMILVETAEDVERLIAPDPNKVAY
                     LTQTTLSIDDANVVIAALRKKFPNIANPPKDDICYATQNRQEAVRELAAQADLVLVLG
                     SQNSSNSRRLAEIANGLGIPSYLIDGVEEIQHLWFRDVETVLITAGASAPEQVVQECI
                     EYLERHFGATFQEETVRKESVHFPLPRSLRQLLPAPAPGDVAAEFDDATEDETSEASV
                     GWH"
     gene            complement(4091..4897)
                     /locus_tag="VHS97_11950"
     CDS             complement(4091..4897)
                     /locus_tag="VHS97_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448962.1"
                     /translation="MFDIEREQLRKTYQLCRLGFTILAVALVLACFISLLDLFSRFDR
                     SLATWIQQSMLYRWLDAPIVWGCLIGATLLFGRWDHASWQRRAGLFLMMNLVDLGLWF
                     INRGDAIGVGAGEFGHEWLREIMGEALGWGEFALISSMSCDYLVHLGVDHARDSDKST
                     RSMAATGAMVWLLLICQLTDWHAGWPLQPHHQFRGLESMLLYHGFKLIWAITLIQVTA
                     LVISAVRQSTEVIAEMDREDQETDLLRSRSDSPQPFETASSYRDQDQRPF"
     gene            complement(5009..5407)
                     /locus_tag="VHS97_11955"
     CDS             complement(5009..5407)
                     /locus_tag="VHS97_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448963.1"
                     /translation="MNEHLIRLRGGWESRPAISSESEQQRLILPVRWGLRDPRQVILT
                     RRFGRPRVQPGSRVLLRMDQVQGIVALLLNGKSIFPVSPEITRYEIELPTLAERNVLV
                     LEIELREPAGEITGAGVEWGMIALVVRTGD"
BASE COUNT         1164 a         1868 c         1628 g         1083 t
ORIGIN      
        1 accttgtcgg gtcgatcgtc cagcgctccg acgggtcgat gattgtgacg tcggcatccg
       61 cgccgccacg cagcgtcccc ttgggtattc ccagcagctg cgccgggttg atcgtcaatt
      121 tgcgcaacgc ttctatccaa ctaaggtgcc caggttcgat caggctcgcg atcgcgatcg
      181 gaatcagagt ctcgagaccg acgatgccaa acggcgcctg gtcaatctca cgcaacttct
      241 tttcacgcgc gtgcggggcg tgatcggtgg caatgatctc gatcgttcca tccttgagcc
      301 cgccgatgac ggcctcgacg tcgctccagg tccgtagcag cgggttcatc ttgaagttgg
      361 aatcgaacga tctcaaccgc tcatcagtga gtgtgaaatg atgtgggcat gcctccgcgg
      421 tcacccgtac cccgcggcgc tgtccttcgc ggaccagctc gaccgagcgg gccgtcgaaa
      481 tgtgctgaat atgcagccgt ccgccagtga tctcggccag gcgtatgtcg cgggcgacca
      541 tgatgtcttc agctgccgcc ggcataccgc ccaaccccag gcgcatcgac tcgaaccctt
      601 cgttcatcac accgccggtg gtcagctcgg gcacctggca atgctgcatg atcacccggt
      661 caaacatctt gcagtattga agcgcccgcc tcatcaaggc cgcggaggca acaggcgctc
      721 cgtcgtcggt aaaggcgacc gcgcccccgg cgaccagttg gccgagctcg gctagctctt
      781 cgccctttcg ccctttgctg actgctccca ccgggtaaac attggcctgc cgcgcccgct
      841 gcgcttgcag aaccacaaac tcggcggccg cctgggtatc gattgccggc aacgtgttgg
      901 gcatgcacgc caccgaggtc accccgccgg ccagggcagc gctcgcgccg gtggcgatcg
      961 tctcgtcctc ttcgtttcct ggctcacgca ggtgcacgtg cacgtcgatc aggccgggac
     1021 agacgatcat cccctttgca tcgatgacaa cctcggcttc ttcatatccg gcgcccaatg
     1081 gcaagatatg cccgcgactg atccagaggt cgccgacttg gtcgaagtcc tgcccagggt
     1141 caatgatccg gccgcccgcg atttgaatgg tcctcaaggc aagtccccct ttcccgtacc
     1201 cgcaatgtgc acgggatcgg cccaaccgtt ctcaattcct gcgatcgaga ggcaggccct
     1261 tggctcccgc ccggccggac agcagataga gcacggccat tcgtaccgcc agcccattgg
     1321 tcacctggtc cagaatcgcc gagtgcttgc cgtcggcgac ctccggcgtc agctcgacgc
     1381 cccgattgat gggtccaggc gcaagcagga gcaagtcttc ccgggcaagt cccaaccgtt
     1441 ccttggtcat catgtaaaaa tgcgagtatt cggcgaccga cggaaacatt cccccctgct
     1501 gtctttcaaa ctggatgcgc agcacgttga tcacatcgca cctcggcaaa acgtcgtcaa
     1561 ggtgataggc aacctcgcag ccaagccgct cccatccctt ggggaccagc gtcggcggtc
     1621 cacacaaaat caccttggcg cctagcttga gcaagcccca gatattggaa cgcgccaccc
     1681 gcgagtgggc gatgtcgccc aagagcgcga ccgtcaaccc gtcgaacttg cccttggcgc
     1741 ggcggatcgt catcaagtcg agcaagccct gggtggggtg ctcgtgtgca ccgtcgccgg
     1801 cattgataat cgagcatttc aggtgctccg ccagcatgtg aggcgtaccc ggcgtgggat
     1861 ggcggatcac cacgacgtcg gcccccatcg cctcgatgtt cctggcagta tcgataaacg
     1921 actcaccttt cgacaggctc gaaccgctcg gcgaaaagtc ttgcgtgtcg gccgatagcc
     1981 gcttggccgc caggctgaag ctcgtccggg tccgggtcga gttttcaaag aacaggttga
     2041 aaacgatccg cccctggagc gcgggcactt tcttgcgact gcgcgtcgag atctcggcga
     2101 acgactcggc cgtatcgagg atcgccacga tctcatcgcg cgataattct tccaagccga
     2161 ggagatgttt tcgactccac ggcgccacgc tctcggcggg gccggcaaag ggcgcgctgg
     2221 tcacggctcg gcactcctcg gcaacggtga cttacggctg ccggccgcca ttttcaccac
     2281 ttcctcgacc ggatcgaccg gctgcagccg cacacccaca tgatcgtcga agttggtcac
     2341 catgtgcaga cccacaacgt cgggctggat gggaatctcg cgatggccgc gatccaccaa
     2401 gaccgccagg cgcacgcacg ccggtcgacc cagatcgcag atggcgttca aggctgcacg
     2461 aaccgtgcgg cctgtgaaca aaacatcgtc cacgagcacg acctcggccc cgtccaggtc
     2521 aaacgggata tcggtgcctc gaagcaccgg ccagcgctgc gttcgcccga tatcgtcgcg
     2581 atagagtgtg atgtcgacgg cgccaacggt gacatcttgt cctagcagcc ggcacagctc
     2641 ttgcgccaac cgctgggcca gcggaacccc gcgcgtccga acgcccacca ggtacagagg
     2701 cacacccacg cgccgggcgt cggcaagctt gcgcgccatc gacgatatca agccaccaat
     2761 ctcatggccg tcgcacagcc tctgctcgcc tggattcatc gcgtgcaagc ctccgctttg
     2821 gggacacgcc cggatggggc gtcggactgg gaatgtagca gagacgctta tctgctttca
     2881 agcatggcgc agggccgccg tgacctggcc cgccctgact cgccttttcg ttggcccggc
     2941 aaacgaatag gatatgggcg tcttgctcca cggaaaccgg aggttgcctc gcgatgaaga
     3001 tcatcctggc gaatccccga ggcttttgcg ccggggtcaa tatggcaatt gaatgcctcg
     3061 agcgcgctct cgatttcttc gggccgccac tgtacgtcta tcacgagatc gttcacaaca
     3121 agttcgtcgt tgaacggttt cgcgaccgcg gcacggtatt cgttgagtcg ctcgatcaag
     3181 tcccgccagg cgctcccctc ctctacagcg cgcatggagt ctcaccccag atccgcgagc
     3241 aagcccgaat acgccagctc gtcgccatcg acgcgacgtg tccgctggtg atcaaggtcc
     3301 acctcgaggc tgtccgttac gcacgtgaag ggtacacaat cgtgctgatc ggccatgagg
     3361 gacacgacga ggtcatcggc accatgggcg aggcccccga acagatgatc ctggtcgaga
     3421 ccgccgagga tgtcgagcgg ttgattgccc ccgatcccaa caaggttgcc tatctcaccc
     3481 aaaccactct gagcatcgac gacgccaacg tcgttatcgc cgccctgcgt aagaaatttc
     3541 ccaatatcgc caatccaccc aaagacgata tctgctacgc gactcagaat cgccaagaag
     3601 ccgtccgcga gctggcggcg caggccgatc ttgtgttggt acttggcagc cagaacagct
     3661 ccaacagccg ccggctcgcc gagatagcga acggtctggg gatcccttcc tacctgatcg
     3721 acggtgtcga agagatccag cacctttggt ttcgcgatgt cgaaaccgtc ctgatcaccg
     3781 cgggagccag tgcgcctgag caggtcgtcc aggagtgtat cgaatacctc gagcgtcact
     3841 ttggcgcaac cttccaggaa gagacggttc gcaaggaaag tgttcacttc ccccttccca
     3901 ggtcgctgcg ccagttgctt cctgccccag ccccggggga cgtagctgcc gaattcgacg
     3961 acgcaaccga agacgaaacg tccgaggcgt ctgtcggctg gcattgaccg gcgggtggac
     4021 gttaaatgct gcccagagtg gaaatggaca tgcccgctcg aaagagagag ttctgtggcg
     4081 cggatgacga tcaaaacggt cgttggtctt gatcgcgata ggacgacgcg gtctcgaacg
     4141 gctgcggtga atcggagcgc gatcgaagca aatcggtttc ctgatcctcg cggtccatct
     4201 cggcgataac ctccgtactc tggcggaccg ccgagatcac gagcgccgtc acctgaatga
     4261 gcgtgatcgc ccagatcagc ttgaatccgt ggtagagcag catactctcg agcccgcgaa
     4321 attgatgatg cggctgcaac ggccagcccg catgccagtc tgtcaactgg caaattagca
     4381 ataaccacac catggcgccg gtggccgcca tcgatcgcgt cgacttgtcc gagtcacggg
     4441 catgatcgac gcccaggtga accaggtagt cgcaactcat gctcgaaatc aaggcgaatt
     4501 cgccccaccc cagtgcctct cccatgatct cccgcagcca ttcgtgtccg aactcgccgg
     4561 cgccgacacc gatcgcgtcg ccgcggttga tgaaccagag ccccagatcg accaagttca
     4621 tcatcagaaa caatccggcc cgtcgctgcc aactcgcgtg atcccatcgg ccaaagagca
     4681 aagtcgcacc aatcaagcaa ccccacacga tcggcgcatc gagccatcga tacaacatcg
     4741 attgttgaat ccaggtcgcc aggctcctgt cgaatctcga aaacaagtca agcagtgaga
     4801 taaagcatgc cagcacgagc gccacggcaa ggatcgtgaa gcccagccgg catagctgat
     4861 aggttttgcg aagttgttct cgctcgatgt cgaacatggg gtacacatct cggcggtggt
     4921 taaagcacca agttcccagc agaacacgag gcaattgatt ctaagccgac ttcctcgcag
     4981 aaaccagagg gtccgggcgc tcttgcgctc aatcgcctgt acgcacaacc aaggcgatca
     5041 ttccccactc gaccccggca ccggtgattt cacctgccgg ctcgcgcagc tcgatctcca
     5101 gaaccaggac attcctctcg gccagagtgg ggagttcgat ctcataacga gtgatttcgg
     5161 gcgagacagg aaaaatagat ttcccattaa gcaacaaagc gacaatgccc tggacctgat
     5221 ccattcgcag caaaacccgg ctgcctggtt gcacgcgcgg gcggccaaac cggcgagtaa
     5281 ggatcacctg ccgtggatcg cgcagtcccc agcggacagg caggatcagc ctctgttgct
     5341 cggattcgga cgagatcgcc gggcgcgatt cccaaccgcc acggagacga atcagatgct
     5401 cgttcacgcg acaagatctc cgaccgggac gactcctgcg cgcctcatgc gccacaggat
     5461 cggcgggttc ggccgacaag cagaacttca aggttcatct ggctttggta tggccttaag
     5521 cgaatagact cattatattc ggcttttccc tggaacaact cggcgcgtcg tgattaactt
     5581 ctgtgcgctt tccttccgct tcctggttgg gtcgactctg gctcgccgat agatttagag
     5641 gtcgccggag gaaatgatgt ttcgagccga tcggctcacc ggcttcggca atctcacatc
     5701 gcgattgaag ttcgtcgcgt atcgctcatg agcaacggcc aat
//
LOCUS       DATAQL010000683         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1139300, whole genome shotgun sequence.
ACCESSION   DATAQL010000683 DATAQL010000000
VERSION     DATAQL010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139300"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..975)
                     /locus_tag="VHS97_11960"
     CDS             complement(223..975)
                     /locus_tag="VHS97_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEX3448964.1"
                     /translation="MKGRSAPRRGFCWAGCHGVDHIFDAWRLTPEGAAIHLGESVAVV
                     ADLHLGYEWARGAAGDLVIAHSLDETLSRLALVLERAKVARLIVAGDLVESPRPCPRT
                     RADVRRLREWLAGRGVSLLALEGNHDRSLAGPLFEQSRPAYSMPMTCTIAGWTIGHGH
                     RPIRGTRTMSGHHHPVLRVEKTSAPCFLVGPGRIVLPALSANAAGCDVARARLPAEWK
                     GRSLRCIVSTGSELLDFGTLFELRRHMRLRKA"
     gene            complement(1023..>2881)
                     /locus_tag="VHS97_11965"
     CDS             complement(1023..>2881)
                     /locus_tag="VHS97_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX3448965.1"
                     /translation="DEAPHRGLSYRRLLRRLKGTITRNRTTVIFANTRAFAEKLTHDL
                     RHELAGGIDPFGAVAAHHSALDAARRREIERALRGGELRAVVTSTSLELGVDIGTADV
                     TVQVGLPGGVARCVQRVGRSGHRRGTGSRGLLLAATPAELAGAIITARASLAGRVEPL
                     RMIRAPLDVVCQQLVSMACAGEQNIDEVFELFRKTGPMAELARADFDACLASLAGELA
                     APAGAFEPEPGANPRWTSSRIWKAKGWFGVRSWRVARWFRSNVGTIHSEETVRVMDAG
                     VAVGTLEAPYAERLISGDRFVLDGRVFKFRRLKNAIVHARPEHGEPNLPRWTSDRQSL
                     SFELAQELAGFREEAARRLVEEGVAPLREWLMTLFELDHGAAAVLIGLFEAQVQWSEV
                     PDSCGILVEESPSPNGDGIIYAFHAPLHRAACEALGRATAARLGRRWGRNLGLCVADL
                     GWSIRLPDEGSAALTHDAILSLLDLAGFADDVLEGLDKGELPARRFQQIAATGLMVLR
                     TAEPGRRVRVGGLNWVSTRLYPLLKAACPDHPLLRETRREVLEDLLDVPAAERWIKGG
                     PAIRFRSLPGLSPFAAAWIESGQADALQFEPPAAALRRLHARLVLQGAEQGS"
BASE COUNT          518 a          988 c          924 g          451 t
ORIGIN      
        1 gcttggcgaa atgcggcatg gttcaacgtt agcatcttcg cagttgataa gaacggggtg
       61 gctggggtcg agctggcgac agccagcgag cccccggcgc ggaagcctcg gatctggggg
      121 cgtcgccctt agggcgtcga ccccagccgc cccctgacgc gtaacctgcg gttgaacctt
      181 gcccgaaaag cgctatacga ccggcgacct cgtcaggctc agctaagctt ttcggagtcg
      241 catgtggcgg cgcagctcaa aaagcgtgcc gaagtcgagc agttccgaac ctgtactgac
      301 gatgcaacga agcgatcgcc ctttccactc ggctggcagg cgggccctcg cgacgtcgca
      361 gcccgcggcg ttggcggaaa gggccggcag tacaatccgg cccggcccga cgagaaagca
      421 tggggcggaa gtcttctcga cccgaagcac agggtgatga tgtcccgaca tcgtccgggt
      481 gccccggatc ggccggtggc cgtgaccgat cgtccagcca gcaatcgtgc aagtcattgg
      541 catcgaatac gccggccgcg attgctcaaa caaagggccg gcaaggcttc gatcgtggtt
      601 cccctcgagg gccagcagag agacgccgcg cccggcaagc cattcgcgca gccggcgcac
      661 gtcggctctg gtgcgagggc acgggcgtgg cgactcgacc agatcgcccg ccacaatcag
      721 gcgagccact ttcgcgcgtt cgagaacaag tgcaagtcgc gacagggtct cgtcgagcga
      781 atgggcaatc accaagtcgc ccgccgcccc ccgggcccat tcgtagccga ggtggagatc
      841 ggcgaccacc gcgacggact cgcccaggtg aatcgcggcc ccttccggag tgagccgcca
      901 cgcgtcgaag atgtggtcaa ctccgtggca gccagcccaa caaaatcccc tcctgggcgc
      961 cgacctgcct ttcatgtttc tgtcccttcg ggatgaagac gcgaggcgca acctcgattt
     1021 gttcatgaac cctgctctgc cccttgcagt accagccgtg cgtgtaggcg ccggagcgcc
     1081 gcggccggtg gctcgaattg cagggcgtcg gcctggcctg actcgatcca ggccgcggcg
     1141 aatggagaaa gccccggcaa cgagcgaaat cggattgctg gtcctccttt gatccagcgt
     1201 tccgcggctg gcacgtccaa aagatcttcg agcacctcgc ggcgagtctc tcggaggagt
     1261 ggatggtcgg ggcacgcggc tttcagcagt gggtaaagac gcgtgctgac ccagttcaag
     1321 ccgccgacgc gcacccgccg cccgggttcg gcggtgcgca gcaccatcag gccggttgcg
     1381 gcgatctgct gaaagcggcg cgcgggcagc tcgcccttgt ccaggccctc gagcacgtcg
     1441 tcggcaaatc cggccagatc taaaagggat aagatagcat catgggtgag tgcggccgag
     1501 ccttcgtcgg gcagacgaat cgaccagccc aggtcggcca cgcacagccc aaggtttctg
     1561 ccccagcgac gaccaagccg ggccgcagtc gcgcgaccca gcgcttcaca cgccgcgcgg
     1621 tgcaaggggg catgaaaggc gtaaattatg ccgtccccgt tcggcgacgg tgattcttcg
     1681 accaaaatac cgcaagagtc gggcacctca ctccattgca cctgtgcctc gaagagcccg
     1741 atcaagacgg cggcggcacc gtggtcgagc tcgaacaacg tcatcagcca ctcgcggagg
     1801 ggagccacgc cctcctcgac cagccgccgg gccgcttcct cgcgaaatcc ggccagttcc
     1861 tgggccagct cgaatgataa tgactgccgg tcgctcgtcc agcgcggcaa gttcggctcg
     1921 ccgtgctctg gccgggcgtg aacgatggca ttcttcagtc ggcggaactt gaaaacgcgg
     1981 ccgtcgagaa cgaaccgatc gcccgaaatc aaccgctcgg cgtaaggcgc ttcgagagtg
     2041 ccgaccgcaa cgcctgcgtc catgacccgc accgtctcct cggaatgaat cgttccgacg
     2101 ttgctgcgga accagcgggc cactcgccag cttcggacgc cgaaccagcc tttggccttc
     2161 cagattcgtg acgaagtcca gcgcgggttg gctcccggct caggctcgaa cgcacccgcc
     2221 ggcgcagcca gctcgccggc cagggaggcg aggcaggcgt cgaaatccgc ccgcgccagc
     2281 tcggccattg gccccgtctt gcggaagagc tcaaaaacct cgtcgatatt ttgctcgccc
     2341 gcgcacgcca tgctgacgag ttgctggcag accacgtcga gtggagcgcg aatcatgcgg
     2401 agcggctcga cccgaccggc caaagaggct cgggccgtga taatggcccc ggcgagctcg
     2461 gcgggtgtcg ctgcgagtaa tagcccgcgc gagcccgtgc cccgccgatg gcccgaccgg
     2521 ccgactcgct gcacgcaccg agccaccccg ccgggcagtc cgacctgaac cgtcacgtcg
     2581 gccgtgccga tgtcgacccc cagctccagg ctcgtgctcg tgacgaccgc ccgtagctcg
     2641 cccccccgca gggccctctc gatctcgcgc cgcctggccg catcaagcgc cgagtggtgc
     2701 gcggccaccg ccccaaaggg gtcgataccg cccgcaagct cgtgccgcaa gtcgtgcgtg
     2761 agcttttccg cgaaagcccg tgtgttcgcg aagatcaccg tcgtccggtt gcgggtgatc
     2821 gttcctttca gccggcgcaa cagccggcga tagctcaacc cgcgatgggg tgcttcgtcg
     2881 t
//
LOCUS       DATAQL010000684         5120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_682033, whole genome shotgun sequence.
ACCESSION   DATAQL010000684 DATAQL010000000
VERSION     DATAQL010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682033"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1331)
                     /locus_tag="VHS97_11970"
     CDS             complement(60..1331)
                     /locus_tag="VHS97_11970"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3448966.1"
                     /translation="MRNLDLLNFVHIILCIYIIPYSAFILITSLAALCGRKTIRPGEG
                     TKPEPSSSRQRFLVVVPAYDEGPSIAKTVLSCKALQYPASLFDVLVIADNCSDDTALQ
                     ARQAGARVLERFDAKKKSKGHAIEYLIETLIQSGEFDSLDALVIIDADSTVHPALLER
                     FSQELERGSDWIQCYDCVGNADQSWRTRLMAYGFSLINGVTLLGLRALGLSAGLRGNG
                     MCISTEGLRRVPWTANGLAEDLEYSWSVRIAGGRIVFIRDVAVYAIMLTKGGIASANQ
                     RRRWEFGRSVLRRKMLGPLLRSHHLGWIQKGAAVVELTMQPAIHLVIVYLVLSVLLLW
                     RLPRMIDQGEYSLLSFVGTTHAIATLGFAIFAVSPLLLDFLAWRYALSLIYLPYYALW
                     KVVVLLRGRPQSWLRTIREGEPIASSRKVSE"
     gene            1720..2757
                     /locus_tag="VHS97_11975"
     CDS             1720..2757
                     /locus_tag="VHS97_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3448967.1"
                     /translation="MLVRLVKSMLDALLIGLGVLFYHVGLAGSIIRLGGGRPRVLMYH
                     ACEESESDFTRGLSINTTPARFSAQLDFLQKYYRIVPLVTLHQEPSPVPAVVITFDDG
                     FRSVYEHAFPCLKARGLPATCYLATDVIANHALIWLNELNWYLHRHRTVAKPLVCQRL
                     GMSPHSSLKRLIEAAIAGYDRGAIVDFLTELRDKTATKPERLAEGTRLYIDRREIQEM
                     SRNRFTFGNHSGSHAVLPRLSQSECREEVGRAKSVLATVPQSIPSLAYPFGHSDEVTR
                     RIARDLGYTTLMNVEGDNSPLDLLQVGRLNVTSCSPAVLFARLELAAPIKFRIKRFFK
                     GLRARFTLRPR"
     gene            complement(2738..3892)
                     /locus_tag="VHS97_11980"
     CDS             complement(2738..3892)
                     /locus_tag="VHS97_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3448968.1"
                     /translation="MIYLDNAATSFPKPEPVYQALDAFARTSLANPGRAGHRMAVAAE
                     RTLDATRHSLNQFFHGAAPERWIFTLNCTDGLNLAIKGIVQPGDHVITTDLEHNSISR
                     PLVALQKTGIISLTRVASESGYVDPDAIRNAITPKTSLVAMTHASNVLGTVQPIAAIG
                     AIVRETGALFLVDAAQSAGVVPIDLRMAPVDMLAFPGHKSLYGPTGTGALYVGPRTDG
                     KLRPWREGGTGGDSSSATQPTLLPYFLEGGTPNVLGVAGLAAGIAWVADRGPDNVREH
                     EVGLLQQVVDWVLASGSWRLAGRWEPQTHVGALSLIAPDSLAPQDLGAILDTSFDIAV
                     RPGLHCAPYIHRALGTFPDGTLRLSPGPFTKPDDISAFLSALSEITAGVV"
     gene            complement(3899..4936)
                     /locus_tag="VHS97_11985"
     CDS             complement(3899..4936)
                     /locus_tag="VHS97_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX3448969.1"
                     /translation="MILDPLDRYSRQIRFPQLGEAGQKALLRSRVTLCGCGALGTVLA
                     NHLARAGVGMIRIIDRDFIETHNLQRQILFDEDDVASNLPKAEAAARKIRAINSSITV
                     EPVVTDLDHTNILDLVGDADLILDGTDNFETRYLINDAAVKLDKPWIFGGVIGSEGQT
                     MTIVPGKTPCLRCLIETAPPPGMTPTCETAGVLGPAVAVIASFEAIEAIKLLAGKHDV
                     LNTHLIMVDVWDWTFRQLKVAGLVGKVDCPCCVRKNFEWLEGAMGSHTTTLCGRNAVQ
                     VAVRRGDPLDFRELAGRLNGISDVRHNAYMLRFATDGYEFTVFPDGRAIIKGTNDVTK
                     ARTLYAQYVGS"
     gene            complement(4946..>5120)
                     /locus_tag="VHS97_11990"
     CDS             complement(4946..>5120)
                     /locus_tag="VHS97_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX3448970.1"
                     /translation="QLSTLYPALRERLFDGDELRRFVNVYVNDEDIRYLEELATPVAA
                     TDEVSIIPAVAGG"
BASE COUNT         1049 a         1520 c         1536 g         1015 t
ORIGIN      
        1 ccttggcact accgcctgaa cgctaaaggt catctcactc cgaggacgta cccaggttcc
       61 tattcagaaa ctttgcgaga agatgcgatt ggttcgcctt cgcggattgt tcgtaaccag
      121 ctctgaggtc gcccccgcag aagcacgacc accttccaca aggcataata gggaaggtag
      181 ataaggctca gcgcgtatct ccatgcgagg aagtcgagga gtagcggact cacggcgaat
      241 atcgcaaatc ccagggtcgc gatcgcatgg gtggtaccga caaacgacaa gagcgaatac
      301 tctccctgat cgatcatgcg ggggagccgc caaagcaaga ggaccgataa cacgagataa
      361 acaataacaa ggtggatcgc gggctgcatc gtcagctcca ccacggccgc ccctttttgg
      421 atccagccaa gatgatgcga tcgcaacagg ggacccaaca tctttcgtct caaaacacta
      481 cggccgaatt cccaccgtcg tctttgattt gcggaagcta tcccgccctt ggtgagcatg
      541 atagcataga ccgcgacgtc gcggatgaag acgattcttc caccggcgat tcggactgac
      601 caggagtact caagatcctc ggccaagccg ttggccgtcc acggcacgcg tcgcaaaccc
      661 tcggtcgaga tgcacattcc attgccacgt aggccggcac tgagcccgag agcgcgcagg
      721 cccaaaagcg ttactccgtt gatcaggctg aacccgtaag ccatcagtcg agtccgccag
      781 gactggtcgg cattgccgac gcaatcgtaa cactggatcc aatcactccc acgctcgagt
      841 tcctgggaga accgctccag cagcgccggg tggaccgtcg agtcagcgtc gatgatgacg
      901 agcgcatcaa ggctgtcgaa ctcgccggac tggatcagag tctcgatcag gtattcgatg
      961 gcgtgaccct tgcttttctt tttcgcgtcg aagcgctcca ggacacgcgc accggcttgc
     1021 cgtgcctgca acgcagtatc gtccgagcag ttgtcggcga tcaccaagac gtcaaagagg
     1081 gaagcagggt attgcaatgc cttgcagctc aaaaccgtct tggcaatact cggtccttcg
     1141 tcatacgctg ggacgacgac cagaaatcgt tgtcgagaag acgagggctc cggtttggtg
     1201 ccctcccctg gccgtattgt cttccggccg catagtgccg caagagacgt gatgaggata
     1261 aatgcagaat agggaattat ataaatacat aaaattatat gaacgaagtt tagaagatct
     1321 agattgcgca tatttcccca ctccgacgaa gggagttggg tggatgatcg ttcaagattt
     1381 tggatcgatg ttatgaccgc gctcgctcga ttgattcctc aagcgcgtaa tcagcgtttt
     1441 ctctaaagaa gaatctcgcg aggccgcacg tcagaccgaa cgagaagggg ttgtggcagc
     1501 ttttccacgc aattctgaag catgatccct gacgggtcag cctcactcag ccccaggtcg
     1561 gctggcagtc tgcccaaaac cggcagatcg gccaagtgta ttctgtcaca cccatgagac
     1621 atatacaaga cggctccagt cgttcctgag aacacgcatg ctgaagcgtc ttcaagggtt
     1681 ggcgaaataa gatcgagtgt cggataccca gtacatcaca tgctcgtgcg gctcgtaaaa
     1741 agcatgcttg atgccctgct aattggtctg ggcgtcctct tttaccatgt cggtttggcc
     1801 ggctcgatca tccgcttggg cggtggcagg ccgagagtcc tcatgtatca cgcctgcgaa
     1861 gagtcggaga gtgatttcac ccgcggtctc tcgatcaaca ccacccctgc tcggttctcc
     1921 gctcagctcg atttcctcca gaaatactat cggatcgtcc cgcttgtgac cctgcaccag
     1981 gaaccgtcgc cggttcctgc ggttgtcatc acattcgacg acggttttcg ttccgtgtat
     2041 gagcacgcgt tcccctgctt gaaggcacga ggcctgccgg ctacttgcta tctcgccacg
     2101 gacgtgatcg cgaatcatgc gctgatttgg ctcaatgagt taaactggta tctccaccga
     2161 catcggaccg tcgccaagcc tctggtctgc caacgactcg gaatgagccc acatagttcg
     2221 ctgaaacggc tgatcgaggc cgcaatcgcc ggctacgatc gcggagcgat cgtcgatttc
     2281 ctcacggaat tgagagataa gacggccacg aagccggaga gactggccga gggaacacgg
     2341 ctgtacatcg atcgtcgcga gattcaagag atgtctcgca accgatttac gttcggaaac
     2401 cattcgggat cgcacgccgt ccttccgagg ctgtcccaat ccgaatgccg ggaggaggtc
     2461 ggccgcgcca agagcgtgct cgctactgtg ccgcaatcga tcccatcgct ggcctacccc
     2521 ttcggccact cggacgaggt aacgcggcga atcgcccgtg atctcggtta cacaaccttg
     2581 atgaacgtcg aaggggataa cagcccgctc gatctgcttc aggtcggacg actcaacgtc
     2641 acctcgtgct caccggcggt attattcgcc cggttggaac tcgccgcccc tataaagttc
     2701 cgtatcaaac gattcttcaa aggtcttcgc gcccggttca cactacgccc gcggtgatct
     2761 ccgacagggc gctcaagaag gccgagatgt cgtcaggctt cgtgaagggc ccggggctca
     2821 agcgcaacgt cccgtcgggg aaggtcccca aggcgcggtg gatataagga gcgcaatgga
     2881 gacccggacg gacggcgatg tcgaagctgg tgtccaggat cgcgcccagg tcttgaggag
     2941 cgagggagtc aggagcgatg agcgagagcg cgcccacgtg ggtctgcggc tcccagcgcc
     3001 ccgccaagcg ccaactgcct gaggctagga cccaatcgac gacttgctgg agcaacccga
     3061 cttcgtgctc gcgcacgttg tccggtcctc ggtcggcgac ccaggcaatg cccgcggcca
     3121 gtccggcgac accgagtacg ttgggcgtgc ccccttccag gaagtagggg agaagagtcg
     3181 gttgcgtcgc actcgacgag tcaccgcctg tccctccctc acgccacggg cgcagcttgc
     3241 cgtcggtgcg cgggccgacg tagagggcac cggtgccggt gggaccgtag agcgacttat
     3301 ggcccgggaa cgccagcatg tcgacgggtg ccatccggag gtcgatcggg accactccgg
     3361 ccgactgcgc tgcgtcgacc aagaacagag cgccggtctc ccgcacgatg gccccgatcg
     3421 ccgcgatcgg ttgaacggtc cccagaacgt tggaggcgtg ggtcatcgcg accaaagatg
     3481 tcttaggcgt gattgcgttg cgaatggcgt cggggtcgac gtagccggat tcagaggcaa
     3541 ctcgggtcaa tgagatgatg ccggtcttct ggagtgccac caatggccgg ctgatcgaat
     3601 tgtgctccag gtcggtcgtg atcacgtggt cgccgggctg gacaatccct ttgatggcca
     3661 ggttcagtcc gtcggtgcaa ttgagcgtga agatccaccg ctcgggggca gcgccgtgga
     3721 agaactggtt gaggctatgc cgcgttgcgt cgagcgtccg ctcggccgca acggccatgc
     3781 gatgtcccgc tcttcctggg ttcgccaggc tcgtgcgggc aaaggcatcc agcgcctgat
     3841 agaccggctc gggtttggga aagctggtgg cggcgttgtc gagatagatc atttagccct
     3901 aactcccgac gtattgcgcg tacagcgtcc gcgccttggt aacgtcgttg gttcccttga
     3961 tgatcgcgcg gccgtccggg aacacggtga actcgtagcc gtcggtcgca aaccggagca
     4021 tgtaggcgtt atgcctgacg tcgctgattc cattgagccg gccggccagt tcgcgaaaat
     4081 cgagtgggtc gccgcgccgc acggcaacct gaactgcatt gcgcccgcac agagtggtcg
     4141 tatgcgagcc catggctcct tcgagccact caaagttctt gcggacgcag cacgggcaat
     4201 cgaccttgcc gaccagcccg gcgaccttga gctggcgaaa cgtccagtcc cagacgtcga
     4261 ccatgatcag gtgcgtattc aatacgtcat gtttgccggc gagcagtttg atggcctcga
     4321 tcgcctcgaa cgacgcgatc acggcgacgg ccggcccgag cactccggcg gtttcgcacg
     4381 tgggcgtcat gccgggcggg ggtgcggtct cgatcaggca gcgcaggcag ggcgtcttgc
     4441 cgggcacgat tgtcatcgtc tgcccttcac tgccgatcac gccgccaaag atccagggct
     4501 tgtcgagctt gacggccgcg tcgttgatca ggtaacgcgt ctcgaagttg tcagtcccgt
     4561 ccagaatcag gtcggcgtcg cccaccaggt cgagaatatt agtgtgatcg aggtccgtga
     4621 cgaccggctc gaccgtgatc gagctgttga tcgcccggat tttgcgtgcg gcggcttcgg
     4681 ccttggggag gttgctggcg acatcgtctt cgtcgaacaa gatctggcgc tggaggttat
     4741 gcgtctcgat aaagtcgcgg tcgataatcc ggatcatgcc gacgccggca cgcgccaggt
     4801 gattggccaa aacggtcccg agcgctccgc acccgcagag cgtgacgcgg ctacgcagaa
     4861 gcgctttctg gccggcctcg cctagctgcg ggaaacgaat ctggcggctg tagcgatcaa
     4921 gtgggtccaa gatcatcacg actccttagc cccctgccac ggctggaata atactgacct
     4981 cgtcagtcgc ggcaactggc gtcgccagtt cctccaggta gcggatgtct tcgtcattga
     5041 cgtaaacatt gacgaaccga cgtagttcgt cgccatcaaa aagccgctcg cggagtgcgg
     5101 gatatagtgt ggagagctgg
//
LOCUS       DATAQL010000685         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3583350, whole genome shotgun sequence.
ACCESSION   DATAQL010000685 DATAQL010000000
VERSION     DATAQL010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583350"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VHS97_11995"
     CDS             <1..966
                     /locus_tag="VHS97_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448971.1"
                     /translation="RQGLGSLVQRAFKQGQNAWNSRLLRVGTTCALDLARTASDRRRL
                     THRHFDRGIMKGRLIMLKIVAAVSTVLVSLGLAAFVPAQPPPDGPPFAKVKAKGKEGK
                     KKAGREPGGDLRKAYDLLRRLRADESALGRTDERLRDWTDRATGFYRDGLRALGAADM
                     LRAREYGAAAHDLARAVDHARNAVRYDRPDPDLPAPSDNFGLEDTRDRARVDLYRAYD
                     RLGWLGTWRAAAASDTYVKAARDLYTAARRDLEAGRDERAGELARAAEAMTHVPEHLA
                     QVGDGVGRAELKPALEPPKAGPDPKTKGYGPPPRGLELPPALPPG"
     gene            1108..2388
                     /locus_tag="VHS97_12000"
     CDS             1108..2388
                     /locus_tag="VHS97_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3448972.1"
                     /translation="MDWRNRIGLYGSYFLGMAAIGFTLPYLPLYLGEKGLSDRAIGIV
                     STLAALSALAQFPIGLWSDRIGWRKPFLVVALAVAAVSTALLREAHGVIWLGFLVILF
                     AENGISRAVVESLSGAEAASLAPPGGVGAALGALRFWKPIGIVLVALLGSWMSEKYGV
                     GAILMPLAVAQGLAVAAALLIRETPGHRESRDSRRQEAQWARQEGMSDASPTSGWLPK
                     DAGLWAFVGAMILFHAANAPGGVYLGLFLKRDLHAPDRMLAYAFAVSMIAWMIVVWPG
                     GRLADRLGRKPLLVAAWAIMALRLGLVAVVRSPEIAVANQALDGVGNGLFAVVAAAWV
                     TDRLADPRRSGEAQVIVGSCLVFGSAIGPAAASFLVGPLGYRGLFLALAGLGAVATFI
                     VLFFVPETLNQVDEDPDGRAMKPMAVTSDLFTVP"
     gene            2457..>2908
                     /locus_tag="VHS97_12005"
     CDS             2457..>2908
                     /locus_tag="VHS97_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX3448973.1"
                     /translation="MTTYPASIPSVSTIFLLTYLALALGRIPGLRIDRAGIALVGAAA
                     MLACGILSMHDAAKAVDYETIVLLFSMMVVVSYLRLAGFFGLATEWIASRFSGAFSLL
                     AVTIALSGILSAFLVNDVVCVALSPLVLHLCQRLKRPPIPYLVGLATAS"
BASE COUNT          449 a          874 c         1000 g          585 t
ORIGIN      
        1 cggcagggcc ttggcagctt ggtgcagcgt gcgttcaaac aggggcaaaa tgcgtggaat
       61 tcgcggcttt tacgagttgg cacgacgtgt gcgttagacc tggctcggac agccagcgac
      121 aggcgcaggc tgacgcatcg acacttcgac cggggaataa tgaagggacg attgatcatg
      181 ttaaaaatcg tagccgctgt ttcgaccgta ttggtcagcc ttgggctggc cgcttttgta
      241 ccggcccagc cgccgccgga tggtccccca tttgccaagg tcaaagccaa gggcaaggag
      301 ggcaagaaga aagcggggcg cgagcccggg ggggacctga gaaaggccta tgatctgctc
      361 cgccgccttc gcgctgacga gagcgccttg ggccggaccg atgaacgact gcgcgactgg
      421 acggatcgcg cgaccggatt ctaccgcgac ggactgcggg cgctcggcgc ggccgatatg
      481 ctccgcgccc gcgagtacgg cgccgccgct catgatctgg cgcgtgcagt cgaccacgct
      541 cgaaacgcgg tgcggtacga tcgacccgat cccgacctgc cagcgccgtc ggacaacttt
      601 gggctggaag atacgcgaga tcgggcccgc gtggatcttt accgggctta cgaccggctg
      661 ggttggctgg ggacgtggcg tgctgccgct gcttcggaca cgtacgtcaa ggcggcgcgg
      721 gatctctaca ccgcggcgcg gcgcgacctc gaagcgggcc gcgacgagcg agcgggcgaa
      781 ctggcccgtg ctgccgaggc gatgacccac gtacccgagc atctggccca ggtcggggac
      841 ggcgtggggc gcgccgagtt gaagccggct ctcgaaccgc cgaaggcggg ccccgacccg
      901 aagaccaaag gctacggccc gcctccgcgt ggattggagc tgccgccggc gttgccaccg
      961 ggctaagcgg aatgccattg cgttagcaac aacttactgc tgcatgtaac gctatactcc
     1021 cctcccccct tgtgggggag ggagacagaa ctcaggccgg atctaagagt attcccaaaa
     1081 ctatttccca aagaggtcac gcgcatcatg gactggcgaa atcgaattgg actttacggc
     1141 tcatactttc tcgggatggc cgccattggc ttcaccctcc cctacttgcc gctgtacctc
     1201 ggcgaaaaag gtctctcgga tcgcgccatc gggatcgtct cgaccctggc ggcactgtcg
     1261 gctctggccc agtttcctat cggactgtgg tcggaccgga tcggctggcg caagcccttt
     1321 ttggtcgtcg ctctggcggt ggcggcggtt tcgaccgcac tcttacgcga ggcgcatggc
     1381 gtcatctggt tgggtttcct ggtgattctg ttcgccgaga acggcatctc gcgggccgtc
     1441 gtcgagagtc tttccggcgc ggaagcggcc tcgctcgcgc cgccgggggg tgtcggggcg
     1501 gcgctgggag ctctacggtt ctggaagccg atcggtattg tgctcgtggc tctactggga
     1561 agctggatgt cggagaagta cggcgtcggc gcgatcctga tgccgctggc cgtggcgcag
     1621 ggactggcgg tcgccgcggc gctcttgatt cgcgagacgc cgggtcaccg tgaatcgaga
     1681 gactcgcgcc gccaggaggc acagtgggct cggcaagaag gaatgagtga cgcaagcccg
     1741 acgagcggat ggctgccgaa agatgctgga ctatgggcgt ttgtgggggc gatgatcctg
     1801 ttccacgcgg cgaatgcgcc tggtggcgtt tacctggggt tgtttctcaa gcgcgacttg
     1861 cacgcccccg atcggatgct cgcttacgcc tttgccgtga gtatgatcgc ctggatgata
     1921 gtcgtgtggc cgggtggccg gctcgccgat cgcctgggac gaaaaccgct tctggtcgcg
     1981 gcatgggcga tcatggcgtt gcgactcggt ctggtcgccg tggtgcgcag cccggaaatt
     2041 gctgttgcga accaagcgct ggacggcgtg ggcaacggtc tgttcgcagt ggtggccgcc
     2101 gcgtgggtta ccgaccggct ggccgatccc cggcgctcgg gcgaggccca ggtgatcgtc
     2161 gggtcgtgcc tcgtcttcgg ctcggcgatc ggaccggcgg cggcgagctt tttggtcggt
     2221 ccacttggtt atcgggggct gtttctggca ttggccgggc tcggcgccgt cgcgacattc
     2281 atcgttttgt tcttcgttcc tgagactctg aaccaggtcg acgaagatcc cgacggacgg
     2341 gccatgaagc cgatggccgt gacgtcggat ctgttcacgg tcccctgaca ttcgcggact
     2401 tgagagggcg gaatcgcacc ctcgccgttt cttgaatctg ggaggaatga ttgcccgtga
     2461 caacgtaccc ggcctcgatc ccttcggtat cgacgatctt tctcctgacc tacctcgcgc
     2521 tggccctggg caggataccc gggttgcgga tcgaccgggc cgggatcgcg ctggtgggcg
     2581 cggcggcgat gcttgcctgc ggcattctgt cgatgcacga tgccgcaaag gccgtcgatt
     2641 atgagacgat cgtcttgcta ttcagcatga tggtggttgt gtcgtatctc cgcctggcag
     2701 ggtttttcgg gttggcgacc gagtggatcg catcgcgctt ctcgggagcc ttttcccttc
     2761 tggccgtgac gatcgcgctc tcgggtatcc tctcggcgtt tctggtcaac gacgtcgtct
     2821 gcgtggccct gtcgccactc gtattgcacc tttgccagcg cctgaagcgc ccgccgattc
     2881 cctacctggt ggggctggcg accgcgtc
//
LOCUS       DATAQL010000686         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4040347, whole genome shotgun sequence.
ACCESSION   DATAQL010000686 DATAQL010000000
VERSION     DATAQL010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4040347"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VHS97_12010"
     CDS             <1..468
                     /locus_tag="VHS97_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448974.1"
                     /translation="PIPSATRQVPGVALAAWAGPEGSSLSIYRALPAPGYTPAMIAEA
                     LANRMENLPELRLLVKRTETVAGTTAARVEVVAPGTGDALAPSGIGTPKAPDGKTLIP
                     TRQVTMGFLRPSGTIYLSWNSPESSYSRIAPEIEATLGSIRFSATGMWSMGSF"
     gene            471..>2144
                     /locus_tag="VHS97_12015"
     CDS             471..>2144
                     /locus_tag="VHS97_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX3448975.1"
                     /translation="MSVQVRPTRDRQGRVYTPAVGARLRPFLWISLIGFALLGANGFY
                     LSSVTALTWFLGATQQTFFYMLMVAFHLLFGFLLIVPFLIFGFAHLATSWRRPNKAAI
                     RFGLALLATALVILVSGLVLVRLGGFEVRDPRAREVGYWLHVLAPLAAIALYVKHRLA
                     GPRIRWEWARRFSTLVAGFVVLMGLLHFQDPRSFGVKGPKEGKQYFYPSEAVTANGKF
                     IPARTMMMDDYCLKCHQDAYAGWFHSAHHLSSFNNKAYLSSVRETRRVALTRDGTTQA
                     ARWCAGCHDPVPFFSGEFDDPDYDDIHNPTSQAGITCTTCHAITNVNNTRGNAAYTIE
                     EPQHYPFAFSDDPILQWINNALVKAKPEMHKKTFLKPVIKETEFCSTCHKVGLPYGVN
                     HYKDFVRGQNHHDPFLLSGVSGHGAQSFYYPDVAKSTCTECHMELKPSTDFGAKDFDG
                     KGGREIHDHVFIGANTGLATILGDEETALKHAKFLSDKKVRIDIFGLRDQGVIEGTLS
                     GPLRPDVPELKAGSRYLVEVVVRTLGIGHPFSQGTVDSNEIWVELTARSG"
BASE COUNT          433 a          675 c          603 g          433 t
ORIGIN      
        1 cctatcccgt cagccacgag gcaggttccg ggcgtggctc tggccgcgtg ggccggacca
       61 gaaggttcgt cgttgtcgat ctaccgcgcg ttgccggcgc ccggctacac acccgcgatg
      121 atcgccgaag cactggcgaa ccgaatggaa aaccttcccg agcttaggct cctcgtcaag
      181 cgaaccgaga ctgtcgcggg aacgaccgcg gcaagggtcg aggtcgtcgc gccaggtacg
      241 ggcgacgcgc tggctcccag cggaatcgga acgcccaagg cacctgacgg caagaccttg
      301 attccaactc gccaggtgac aatgggtttc ttgaggccgt cagggacgat ttacttatcg
      361 tggaattcgc ctgagtcgtc gtacagtcgg atcgcaccgg agatcgaggc cactctcggc
      421 tcaatccgct tcagcgccac cggaatgtgg tcgatgggga gtttctgacc atgtccgtac
      481 aggtacggcc cacgagagat cgccaaggca gggtttacac gccggccgtc ggcgcgcggc
      541 tgcggccctt tctatggatc agcttgatcg gctttgcctt actgggcgca aacggatttt
      601 atctctcgag cgtgaccgcg ctgacgtggt ttctcggggc cacgcagcaa acatttttct
      661 acatgctcat ggtagcattt catctgctct ttggcttcct cctgatcgtc cctttcttga
      721 tcttcggttt cgcgcatctg gcgacatcat ggaggcggcc caacaaggca gcgatccgtt
      781 tcgggctggc actgcttgcc accgctttgg tcatcctggt ctcggggttg gtcctggtga
      841 gactgggagg attcgaggtc cgcgatccca gggctcgcga agtcggttac tggctgcatg
      901 tgctcgctcc gctggccgcc atcgcgcttt atgtgaagca tcgtctggca ggaccgcgga
      961 tccgctggga atgggcaagg cgcttcagta cgttagtcgc cggctttgtt gttctgatgg
     1021 gccttctcca cttccaggac cctcggtcgt tcggcgtcaa ggggcccaag gaaggcaagc
     1081 agtacttcta tccatcggaa gccgtcaccg ccaatggcaa gttcatcccc gcgcgaacca
     1141 tgatgatgga tgactactgc ttgaaatgcc accaagatgc ctacgccggc tggtttcact
     1201 cggcccacca cctcagctca ttcaacaaca aagcctatct gtcaagcgtg cgcgaaaccc
     1261 gacgtgtagc gctcacacgc gacggtacaa cccaggccgc gcgctggtgc gccggctgcc
     1321 atgatccagt acccttcttt tcaggcgagt tcgacgatcc cgattacgac gacatccaca
     1381 atccgaccag ccaggccggc atcacctgca cgacttgcca tgccatcacc aacgtcaaca
     1441 acacacgcgg caacgccgcg tacactattg aggagccgca gcattatccc tttgccttca
     1501 gcgacgaccc gatcttgcaa tggatcaaca acgcgctcgt gaaggccaag cccgaaatgc
     1561 acaagaagac cttcttgaaa ccggtcatca aggaaactga attctgttca acctgccata
     1621 aagtcggcct tccctacggt gtcaaccact acaaagattt cgtgcgcgga cagaaccacc
     1681 atgacccgtt tctcttatcg ggcgtgtcgg gccacggagc gcagagcttc tattaccccg
     1741 atgtggccaa gtcaacctgc accgagtgcc acatggagct aaagccatcg accgattttg
     1801 gcgccaaaga cttcgacggc aaaggaggcc gcgagatcca tgatcacgtc tttatcggcg
     1861 ccaacaccgg cctggcgacc atcctgggcg acgaggagac cgctctcaag cacgccaagt
     1921 tcttgagcga caagaaagtc cgtatcgaca tcttcggcct gcgcgaccaa ggcgtgatcg
     1981 agggcacgct ttcgggtccg ctccgtcccg acgtacccga gctcaaggca gggagccgtt
     2041 acttagtcga ggtcgtcgtc agaaccctcg gcatcggcca ccctttcagc cagggaaccg
     2101 tcgattccaa cgagatctgg gtcgagctga ccgcacgttc ggga
//
LOCUS       DATAQL010000687         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2819316, whole genome shotgun sequence.
ACCESSION   DATAQL010000687 DATAQL010000000
VERSION     DATAQL010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2819316"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="VHS97_12020"
     CDS             complement(<1..1530)
                     /locus_tag="VHS97_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3448976.1"
                     /translation="MSVATRKEGKSLFSRGRAQSKGQRDRLGAREVLAWAVWFGLAAG
                     LLEVATRVLCRAIDPTGRLYLMSKHFVWLTPLASMVLFLGLGLLLAGMTRAWPRLGAR
                     LSLRLLCALAIQPMLMVAVPRILPAAWFILAWGIASRLVPLLESQPAKTRRLLLAYGL
                     PALLGLVLIIAGSVFGEEWLKQARESRRALPPAGSPNVLFVVLDTVRTDHLSLYGYPR
                     STTPTLKRLAENGIRFDRARATAPWTLPSHGSFFTGRWPHELGAEWLTPLRPGAPLLA
                     QYMGSRGYATAGFVANTGYCSYETGLARGFTHYEDYILKRLAPFQMPILVKGLLGRIF
                     AMSTAHGPDPLHYVPEMVERWFYAGNRKDAESINRAFLDWLTRRPEQARPFFVFLNYL
                     DAHAPYKLPEGAQHRFGHRPKSREEIRVIYDGWDLIDKLTLPRFYQTMARDAYDSCLA
                     YLDEQLGVLFDELQRHAVLDNTVVVITSDHGEGLGEHDLFDHGESLYSTELDVSLLIL
                     LP"
     gene            complement(1611..2579)
                     /locus_tag="VHS97_12025"
     CDS             complement(1611..2579)
                     /locus_tag="VHS97_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3448977.1"
                     /translation="MKIVILDGRPLAPDRAAWSGLNRLGEVEYHDFTPPDDVPARARG
                     ATVLVTNKAPVRAPLIETAAELRFITVTATGYDCVDVTAARQRGIPVSNVPEYSTHSV
                     AQFVFALLLELCEYAAKHAEAVKAGEWTSQPDFALRKTPLYELAGKTMGIVGLGRIGR
                     QVAVIAKAFGMTVLASKSSRPSPSSNDPIVRCDLDELFARADVISLHCPLTTETEGLV
                     NRQRLARVKPTAFLINTARGALVVEPELAEALNQGRLLGAALDVVSQEPIRPDNPLLG
                     ARNCLITPHIAWATEEARARLMEATVANVAAFLSGQPINVVNLPRG"
BASE COUNT          573 a          838 c          843 g          509 t
ORIGIN      
        1 cgggagcaag atcaacagcg aaacgtcgag ctccgtgctg taaaggcttt cgccatgatc
       61 gaacagatcg tgttccccca gtccttcacc gtgatcggat gtgatcacaa ccacggtatt
      121 gtcaagtaca gcgtgacgtt gcaattcgtc gaatagcacg ccgagctgct cgtccaggta
      181 ggcaagacaa ctgtcgtagg catcacgagc catggtctgg taaaaacgcg gcaatgtcaa
      241 cttatcgatc aagtcccagc cgtcgtaaat gacgcgaatc tcttctcggc ttttgggcct
      301 gtgcccgaag cggtgttgcg ctccttccgg cagtttgtac ggcgcatggg cgtcgaggta
      361 attcaagaac acaaaaaagg gacgcgcttg ctcgggccgg cgagtcagcc agtcgagaaa
      421 cgcgcgattg atcgactccg cgtctttgcg attgccggcg taaaaccacc gctccaccat
      481 ttccggcacg taatgcaggg gatccggtcc atgggcggta ctcatcgcga agatccgccc
      541 cagaagtccc ttgaccaaga tgggcatttg aaagggagcg agcctcttga gaatgtaatc
      601 ctcgtagtgg gtaaaaccac gggccagacc ggtttcatag gaacagtaac cggtgttagc
      661 gacgaagccc gctgtcgcgt aaccgcgaga tcccatgtac tgggccaata gtggagcgcc
      721 cggtctcagg ggcgtcagcc actctgcgcc gagctcatga ggccagcggc ccgtgaagaa
      781 gctgccgtgc gaaggaagcg tccatggggc ggtcgcacgg gctcgatcga atcgaattcc
      841 gttctcggcc agtcgcttca aggtcggagt ggtcgaccga ggataaccgt acaggctcag
      901 gtggtcggtc cggaccgtgt ccagcacgac aaagagaacg ttcggcgaac ccgccggcgg
      961 cagagccctg cgcgactcgc gcgcttgctt gagccactct tcaccgaata ccgagcccgc
     1021 aatgatcaat accaatccca acagagccgg aagaccgtac gctaacaaca accgcctcgt
     1081 tttggccggt tgcgactcga gcaacggaac caaccgtgat gcgatccccc atgccaggat
     1141 aaaccaggcc gccggtaaga tccgcggcac cgcgaccatg agcatcggct gaatcgccaa
     1201 cgcacagagc agcctgaggc tgagccgcgc acccagccgc ggccaagccc gggtcatccc
     1261 ggccagcaag agtcccaggc ccaggaatag caccatactt gcgagtggcg tcaaccagac
     1321 aaagtgcttg ctcatcaagt acaagcgccc ggtcggatcg atcgcgcggc acaacacgcg
     1381 cgtcgccact tcgagtaatc ctgcggcgag accaaaccac acggcccaag ccagcacctc
     1441 gcgcgcgccc aggcgatcac gttgtccctt ggattgcgcc ctacctctgg aaaaaaggga
     1501 ctttccctcc ttgcgcgtag ccacgctcac cgtcttgatt cctcgtctcg aggagcggaa
     1561 aaaccacgct cgttggtcgc ggagcgcgaa tgtctatttt atcaaaccct tcaccctcgt
     1621 gggagattga caacgttgat cggctggccg gagaggaacg cggccacgtt tgccaccgtc
     1681 gcctccatga gtcgtgctcg cgcctcttcc gtagcccagg cgatatgagg tgtgatcaag
     1741 caattgcgcg ctcccaagag cgggttgtct ggtcggattg gctcttgaga gacgacatcg
     1801 agggcagcac ccagcaaccg gccttgattg agggcctcgg caagctcggg ctcgacgacg
     1861 agcgcgcctc gggctgtgtt gatcagaaag gccgtcggct tgacccgcgc caggcgctgg
     1921 cggttgacca gtccctcggt ctcggtcgtc agcggacaat gcaagctgat cacgtcggcg
     1981 cgagcgaaga gctcgtcgag gtcgcatcga acgatcgggt cgttggacga gggggacggg
     2041 cgagatgatt tcgaggccaa gactgtcatg ccgaaagcct tcgcgatcac ggccacctgc
     2101 cggccgatcc ggcccaggcc gacgattccc atcgtcttgc cggccagctc gtacaggggc
     2161 gtcttccgca aggcgaagtc gggctggctt gtccactcgc cggccttgac cgcttcggca
     2221 tgtttggcgg cgtactcgca cagttcgagg agaagcgcaa agacgaactg ggctacggag
     2281 tgcgtgctgt attccggcac gttcgagacg ggaatgccgc gctggcgggc cgcggtcacg
     2341 tcgacgcaat cgtatcccgt cgccgttacg gtgatgaacc ggagttcggc tgcggtttcg
     2401 atcaacggtg cacgaacggg agccttgttg gtgaccagga ccgtcgcgcc gcgagcgcgg
     2461 gcgggtacgt catcgggcgg tgtgaagtcg tgatattcga cctcgccgag ccgattgaga
     2521 ccagaccacg ccgcacggtc aggggccagc ggccggccat cgagtatgac gattttcacg
     2581 ggtctcttgc ctcttccgtt tggtgtcagc tcggtgccgg aaagcctgtt ttggtcggaa
     2641 ttgcagtata acggcgtttg cggcctcttt ggatctcaga cttcaaagga tcgctcggaa
     2701 gcccatgagg ccgatcatcg agcgccatgc gcaactgcaa gcgcgacaat gatttgattc
     2761 caa
//
LOCUS       DATAQL010000688         4855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_682680, whole genome shotgun sequence.
ACCESSION   DATAQL010000688 DATAQL010000000
VERSION     DATAQL010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682680"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..865
                     /locus_tag="VHS97_12030"
     CDS             92..865
                     /locus_tag="VHS97_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3448978.1"
                     /translation="MSARESPWFERWFDFLAFVGRTCARAPSAIVYRLPEVIRQFEQV
                     AVKSLPIVLGAGLSVGLVAWLQTHRLLVAHGAESTLPSFLAVAVLVEIGPILAGLLVA
                     ARMGAGLAAELSSMVLNEEIDARIVLGADPVPTLVAPRAIACALAVPLLTVIIDASAL
                     LGSLAAELTVGRSHSTLFWNKTLVFLRLSDVVPATLKTAIFGLVVATIACWTGLNAER
                     STESVGHAAIRGVVRSILGVFAANVVMVPGIQFAADALG"
     gene            945..2054
                     /locus_tag="VHS97_12035"
     CDS             945..2054
                     /locus_tag="VHS97_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX3448979.1"
                     /translation="MTANKWRAATNAGFVVAVLALGCFGLYQVAGRQWQVQPTFRVRV
                     QFDTIAGLEVGHRVRLQGIDAGVVERVIAPAQPGQPVELVLRVDSRLKHLIRTDAVAR
                     IVAEGLVGAKVVELSPGRPDAPALGELDRIASERPTEVNELLKKAASSLARIDAATLA
                     AEQGLGELAAIVGSIRRGEGSLGKLVRDETAYDNLLDLSRRGERALTDLDENLAALKD
                     TWPLSRYFDHRAYLDRDRVLYQPGAQRHSRAIRAEELFEPGRSVLTPVGQNRLDEIGR
                     WCNQSTRPTSEVVVAAFTDDNRDADLAEILTQEQAETVRKYLVQRHSIQSAGWFKSRK
                     VAAVGFGAHLPRTLDLAPVQAPSRRVEIFVFTPQT"
     gene            2214..4646
                     /locus_tag="VHS97_12040"
     CDS             2214..4646
                     /locus_tag="VHS97_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448980.1"
                     /translation="MEQLLLKERFKEAVKQAKLCYNEESTPEHHLLLERAYCLRARQL
                     IEFGMGSSAVEVARHLLEFGVTGGEWVPEIVRLLMDLGLRDQAFEIQERLGEPELKNQ
                     LIALAADRAVIHPDGCSDQSGEIARDVMLIRESLTRLQARDEAGALLLLRDLARSSVL
                     SEWKFFVRGLAAYYRGHTVEIKANWDRLDPKRKAYRIARRLLEFMEVDGNDTAGERIQ
                     TREKLAYREPILDRLRELNTVTASQDWDKIFRLVGSLRQSLRRIDPKLAQRLTVALAG
                     TVIREAARLDPSDTKRLLNGFTRAAEPTAIDPRWNRLWAITWDGSDDVPSDVSLDYWG
                     KFLEDLKTNPALGDSERALAQAMVWNHMAKAARHLVAELDDDGDSGPFGLPMSVRPRS
                     GPNSVEVLAAKKRVVDCLEKSLELAPLYKPTYELLVEVFRDWDATDSLVTAAHRLLAK
                     FPDDLDTLTLLAHQYTKNDDPAAALPLVERARVLKPLDESLRELEWKIRLDLARSHAL
                     GKRWNQGRAEFVAALKLHPDSDTNYHFLAQRVMFEAKAGERDLSNGYLEQARACLNEP
                     APLWLALAIESIRYRMTKATKDGYAKLWEADLKLKRHSETGGLMAALLDGLWKAGIEY
                     TGRATHIKKVAAYVGPGSRLKYRQSDIENICEFLGHTNQKVATIEKLVAVGLKQYPRS
                     PLLNFRAALLETGKGFFSLGSVRARHFLDTALKLAQASTDPKENVLVTPIKKSLTLLS
                     EMEKLPLGLPSFGGSSSGFPFAGFADFFDDFDDDDDDDDDDGDDDDFYRRPASSPRPA
                     RGTKKPKGRKTR"
BASE COUNT          937 a         1441 c         1550 g          927 t
ORIGIN      
        1 acgaattacg atgaaaacga ggggatgagg accgatagaa tgactatcgt tgatcttggt
       61 gacgagcccc gcgtcgcagg ggagccccgg catgagcgcc cgtgaatcgc cttggttcga
      121 gcggtggttc gacttcctcg cgtttgtcgg tcgcacgtgc gcgcgggcgc cgtcggccat
      181 cgtttatcgg ttgccggaag taatccgaca attcgagcag gtggccgtca aaagtttgcc
      241 gatcgttctg ggagcgggct tatccgtggg ccttgtagcc tggcttcaga cgcatcgtct
      301 gctggtcgct cacggagccg agtcgacgct gccgagtttt ctggctgtgg cggtcctggt
      361 ggaaatcggg ccgatcctgg cggggctatt ggtcgcggct cgcatgggag ccggcctggc
      421 ggccgagctg agctcgatgg tcttgaacga ggagatcgac gctcgaatcg tcctgggcgc
      481 ggacccagtg ccgacgctcg tggcaccacg agcgatcgcc tgtgcgctgg ccgtcccgtt
      541 actgacggtg attatcgatg cctcggcgct actgggcagc ctcgccgccg aactcaccgt
      601 cggcaggtcg catagcacgc tcttctggaa caagacactg gtgtttctca ggctttccga
      661 cgttgttccc gcaacgctca aaacggcaat ctttgggttg gtggtggcca cgatcgcctg
      721 ctggactggt ttgaacgccg agcgctccac cgagtctgtg ggccacgctg cgatccgggg
      781 cgtcgtccgc tcgatcctgg gcgttttcgc cgccaatgtc gtcatggttc ccggtatcca
      841 atttgccgcc gacgccttgg ggtaatgtgt ggtcgaaccg cgatccgctt gaattagcag
      901 caatttactt tgcggagcat gagacgaaga gaggagcgac cgcgatgacg gcgaacaaat
      961 ggcgggcggc gacgaacgcg ggatttgtgg tggcggtgct ggccctcggc tgttttgggc
     1021 tctatcaggt ggccggccgg cagtggcaag tgcagccgac cttccgcgtc cgcgttcagt
     1081 tcgacacaat cgctggtttg gaggtcggtc accgcgtccg gctgcagggg atcgacgcgg
     1141 gcgtcgtcga acgggtgatc gcgcctgccc agccgggcca gcctgtcgag ctggttctgc
     1201 gtgtcgatag tcggctcaag cacttgattc gtaccgacgc ggtagcccgg atcgtcgcgg
     1261 aaggcttggt cggggcaaag gtcgtcgagc tgtcgcccgg ccgacccgac gcgccagctc
     1321 tgggcgagct ggaccggatc gcctccgagc ggcccactga ggtgaacgag ctgctcaaga
     1381 aggcagcatc gtcgctggct cgtatcgacg ctgccaccct ggcagccgag caaggactgg
     1441 gcgagctggc ggcgatcgtc ggctcgattc gccgtggcga ggggagcctg ggcaaactcg
     1501 tccgtgatga gacggcctat gacaacctgc tcgatctttc ccgtcgtggc gagcgcgctt
     1561 tgaccgacct ggacgaaaac ctggcggctc tgaaagacac ctggccgctt tctcgctatt
     1621 ttgaccatcg cgcctatctc gatcgcgacc gcgtgctcta tcagccaggc gcccagcggc
     1681 acagccgcgc cattcgagcg gaagaactct ttgaacccgg ccgctcggtc ttgaccccgg
     1741 tgggccagaa ccggctcgac gagatcggcc gttggtgcaa tcagtccacg cggcccactt
     1801 ccgaggttgt ggtcgccgcg ttcaccgacg acaaccgcga cgccgatctg gcagagatcc
     1861 tgactcaaga acaggccgag acggtgcgca aatacctggt ccagagacac tccatccagt
     1921 ccgccggctg gttcaagagt cgtaaggttg ccgcggtcgg cttcggcgcg catctgcccc
     1981 gcacgcttga cctcgccccc gtccaagcgc cctctcgacg cgtcgagatc tttgtcttca
     2041 cgccgcaaac gtagctaatt gaaggcttgg accgtatgac gatgatgggt agaatgccgg
     2101 tctgttaagg tgaacgagcc gccaatgcga gattgcagca cgacatgctt caagagcact
     2161 cggcccaaac accctcggcg accacatctg cgagtagtca agcctgcttg gaggtggaac
     2221 agctgctcct gaaagagcga ttcaaggaag cggtaaagca ggcgaagctc tgttacaacg
     2281 aagagagcac tccggagcat catctgctgc tcgaacgagc gtactgtttg cgggctcggc
     2341 agttgatcga gtttggaatg ggctcatcgg cggtcgaggt ggcccgccat ttgctcgaat
     2401 tcggcgtaac cggcggcgag tgggttccgg aaatcgtccg gctcttgatg gatcttggcc
     2461 tgcgcgacca ggcctttgag atccaggaac ggctgggcga gccggagttg aaaaaccagc
     2521 tgatcgcgct ggcggccgat cgagcggtca tccatcccga tggatgtagc gaccaatcag
     2581 gcgagatcgc gcgcgatgta atgctgattc gagagtcact gacgcgttta caggccagag
     2641 acgaggccgg cgcgctcttg cttttgcgcg acctggcccg cagttcggtc ttgagcgaat
     2701 ggaagttttt cgtacgcggc ctggccgctt actatcgcgg acatacagtc gaaatcaaag
     2761 ccaactggga tcgcttggac cccaagcgga aggcgtaccg aatcgcacgg cgattgctcg
     2821 agttcatgga agtagacggg aacgacaccg ccggcgagcg cattcaaaca agggaaaagc
     2881 tggcgtaccg tgagccgatt cttgaccgcc tccgagaatt gaacacggtg acggccagtc
     2941 aagattggga caagatattc cgcctggtcg gttcgctccg ccaatcgttg cggcggattg
     3001 atcccaagct ggcccagcgg ctgacggtcg ctttggccgg aacggtgatc agggaggcgg
     3061 cgcgcctcga tccttccgac acgaaacggc tcttgaacgg cttcacccgg gcggccgaac
     3121 ccaccgcgat cgacccgcgc tggaaccggc tctgggcgat tacgtgggat gggtccgacg
     3181 acgtcccatc agacgtttct ttggattact ggggcaagtt tctcgaagac ttgaagacga
     3241 accccgcttt gggtgactcg gaacgtgcgt tagcccaggc catggtctgg aatcacatgg
     3301 ccaaggccgc gcgccatctg gtcgccgagc ttgatgacga cggcgatagc ggaccgtttg
     3361 gcttgccgat gtcggttcga ccccgatcgg gacccaactc ggtggaggtc ttggctgcca
     3421 aaaaacgtgt ggtcgactgc ctggaaaaaa gcctcgagct cgcgcccctt tacaagccca
     3481 cgtacgagtt actggtcgag gtctttagag actgggacgc caccgacagt ctcgtgaccg
     3541 ccgcgcaccg gttgctggcg aagtttcccg acgatctgga cacgctcacg ctgctggccc
     3601 atcagtacac caaaaacgat gacccggcgg ctgctctgcc gctggtagag cgggcgcgcg
     3661 tgctgaagcc gctcgacgag tcactccgtg agctagagtg gaagatccgc ctcgacctgg
     3721 cgcgcagcca tgcgcttggc aagcgttgga accaagggcg agccgagttc gtcgccgcct
     3781 tgaagctgca tcctgacagc gatactaatt atcactttct ggcgcagagg gtcatgttcg
     3841 aggccaaggc cggcgagcgc gacctgagca acggatatct cgagcaggcg cgggcctgcc
     3901 tgaacgagcc cgcaccgctc tggctggcgc tcgcgatcga gtcgatccgc tatcggatga
     3961 ccaaagcgac caaggacggt tacgccaagc tttgggaggc ggacttgaag ctcaaacgcc
     4021 acagcgagac cggcggcctg atggccgcgc tcttggatgg gctctggaaa gcgggcatcg
     4081 aatataccgg ccgagcgacg cacatcaaga aagtcgccgc ttatgtcgga cccggctcgc
     4141 ggctcaagta tcggcaaagc gatattgaga acatctgcga gtttctcggc cacactaacc
     4201 aaaaggttgc cacgatcgaa aagctggtcg ccgtgggctt gaagcaatac ccccgttcgc
     4261 ccctgttgaa cttcagggct gcgctgctcg agacgggaaa aggatttttc tcgctcggaa
     4321 gcgtccgtgc gcggcatttc ctcgacaccg ccttgaaact ggcccaagca tcgaccgatc
     4381 ccaaggaaaa cgtgctggtc acccccatca agaagtcgct cactctactc agcgaaatgg
     4441 aaaaactgcc gctcggtctg ccgtcattcg gtggcagttc gtccggtttc ccgttcgcgg
     4501 gtttcgcaga tttcttcgac gattttgatg acgacgacga cgacgatgat gatgacggtg
     4561 acgacgacga tttctaccgc cggccggcat cgagtccacg accagcccga gggaccaaga
     4621 aaccgaaagg gcgaaagacg aggtaaaggg gtaggtcggg ctttccaacc cgacgtcggg
     4681 cacgtatcga accgagaaac cgaaaggacg caagaggggg taaagggaag tgagcaagcc
     4741 ttacgacgtc atggggctgg ctgctggtgc cagcgaaccc gagatcctgc agcgttatct
     4801 cgagctcgtc cgcgagtttc cccccgaccg cgcaccggag cgtttcgccg ctgtt
//
LOCUS       DATAQL010000689         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2972231, whole genome shotgun sequence.
ACCESSION   DATAQL010000689 DATAQL010000000
VERSION     DATAQL010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2972231"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1365
                     /locus_tag="VHS97_12045"
     CDS             97..1365
                     /locus_tag="VHS97_12045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3448981.1"
                     /translation="MIGGAEKVLSYLARALAVEGADVTVLTSQMPGMMLPAHEDLPVT
                     LVKNGRSKAVASAGRLAVVRLETSRLRFWGTWRYMHNLTRWFYENPVDLAYVSMLKHD
                     AYSVIGAGERLEFPVVLRPEGAGATGDIAWQSWGNFGRMIGRRCRRASAFVSISKAIE
                     QELRDSYRAGTMRPVRSKRVDHRKEESPRIVAIPNGVPVPDLAWQRRRDWRVTPRAVF
                     VGRLATEKGLDVLVAAWPLVRARYPEAQLSLIGDGPQRPALEQMIGTLGMMLGPGQAV
                     DMPGAVPEPSEVMRDADLFILPSREEGMSIALLEAMALGIPLVASSIAGNRRLVSDFK
                     HGRLAPPDDPKALAGVIIDQWDNFDRAFHMSRAARSRVEQEFSIQAVAHKHLALFEDI
                     LKNWRQRERRPLVPNAHNPRTKDQGPRTED"
     gene            1370..2485
                     /locus_tag="VHS97_12050"
     CDS             1370..2485
                     /locus_tag="VHS97_12050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3448982.1"
                     /translation="MLNVLQLIPTLDRSGAEKQMVLLAKGLPRDRFRVEVATLTRLGP
                     LEGELRSAGIPVTAIGKRLKFDPFALARLMRFLKSRKFDVVQTWIFAANTYGRVGARL
                     AGVPVVVVAEMAADLWKGRYEQFVDRRLAPWCDRLVGNSNAVVDFYRKLGLTDDRLEM
                     IYSGTDDEPPACDPRVLRSEFGFAPEAPLVLFVGRLAEQKRVHDLLKALDLLQHVQQD
                     LRSLIVGEGPLRDYLEDTAHAYDLDGRLRFLGHRDDVPRLMAAADVIVLPSAYEGLPN
                     VVLEAMRFGKPVVATAAPGTTEVVVDGETGVLVPVGNVMLLARAIRDVVRNPVEAARM
                     GEAGRARAESLFRVDTMVEKFAELYETLAQSKGIVVS"
     gene            complement(2529..>3173)
                     /locus_tag="VHS97_12055"
     CDS             complement(2529..>3173)
                     /locus_tag="VHS97_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3448983.1"
                     /translation="LTSIVMVSEMTGSYSLLVPLMLACGLNMAISRRWTIYEEQVSTP
                     IDSPAHQGDFVVDVLEQLSVSDVGIRSVGIATIPASMPFRELIHLVAQSTETLFPVVD
                     GNGRLTGIFTLHDLRLALVGSEWGPLVLADDLAYRPVLSVTPEDNLHTALRRMTEINT
                     DEIPVVEPGDSTHLLGLMSRRDLTLAYTSLIESLRSNGPNTSKLNRAEEPVQTR"
BASE COUNT          602 a          883 c         1036 g          652 t
ORIGIN      
        1 actgtccgac caatgaccaa tgaccaatga ccgctgccta cttcgatgcc atcgcgtcct
       61 cttaaactgg ctttggtcac gcggcgcttt ccgccgatga tcggaggcgc tgagaaagtc
      121 ttgagctatc ttgcccgcgc cttggccgtc gagggagctg atgtgacggt gcttacctcg
      181 cagatgccgg gcatgatgtt accggctcac gaagatttac cagtcacctt ggtgaagaat
      241 ggccgttcga aggctgtcgc ttccgcaggg cggctcgctg ttgtgcgact ggaaacctcg
      301 cggctacggt tctggggaac gtggcgctac atgcacaatc tgacgcgatg gttttatgag
      361 aatccggttg accttgccta cgtctcaatg ctcaaacacg acgcttacag tgtaattggc
      421 gcgggggagc ggttggaatt tccggtcgtg cttcgtccgg agggagctgg tgcgacgggc
      481 gatattgcct ggcaatcgtg gggcaacttc ggccggatga tcggccgccg ctgccgccgg
      541 gcaagcgcgt ttgtgtcgat ctccaaggcg attgagcaag agctgcgaga ttcctatcga
      601 gccggaacga tgcggcctgt aaggtcaaag cgcgtggatc accgtaaaga agaatcaccg
      661 cggattgtag cgattcccaa cggcgtcccg gttccagacc tcgcctggca gcgcaggcgc
      721 gactggcggg ttacacctcg ggccgtcttc gtcggccggt tggccacgga aaaggggctc
      781 gatgtgcttg tagctgcctg gccattggtg cgagccagat atccagaggc ccagctgagc
      841 ttgatcggcg atgggcccca gcgcccggcc ctggaacaga tgataggaac tctgggaatg
      901 atgctaggac cgggccaagc cgtcgacatg cccggcgccg tgcctgagcc gagtgaagtg
      961 atgcgcgacg ccgatctgtt cattcttccc tcgcgcgagg agggaatgag tatcgcgcta
     1021 ctcgaggcga tggcgctcgg tattcccctg gtcgcttcgt caatcgccgg caaccgccgg
     1081 cttgtcagcg atttcaagca cggtaggctg gcgcctcctg acgatcccaa ggctctcgca
     1141 ggggtcatca tcgatcaatg ggacaatttc gaccgcgcct tccacatgag ccgcgctgcg
     1201 cgcagtcgcg tcgaacaaga attctcgatc caggcggttg cccataagca cctggcgcta
     1261 ttcgaagaca ttttgaagaa ctggcggcag cgcgagcgac gtcctttggt gcctaatgcg
     1321 cataatccga ggaccaagga ccaaggacca aggaccgagg actaaggact tgctcaacgt
     1381 tctgcaactg ataccgacac ttgaccgctc gggcgccgag aagcagatgg tgctgttggc
     1441 caaagggttg ccgcgcgatc ggtttcgggt cgaggtcgcg acgctcaccc ggctgggtcc
     1501 gctggaggga gaactgcgta gtgcgggtat tccggtcacg gcgattggca aacggctcaa
     1561 gtttgatccg tttgcgttgg cgcggctcat gaggtttctc aagagccgga agtttgacgt
     1621 ggtacagaca tggattttcg cggcgaacac ctacggccgg gtgggggcaa ggttggcggg
     1681 agttccggta gttgtggtcg cagagatggc ggccgatttg tggaaaggtc ggtacgagca
     1741 atttgtcgac cgacggctgg caccttggtg tgatcgcctg gtcggcaact cgaacgccgt
     1801 ggtcgacttt tatcgcaagc ttgggctgac agatgaccgg ctggagatga tctattcggg
     1861 aactgacgac gagccgcctg catgtgatcc acgcgtgctc cgctccgaat tcggatttgc
     1921 acctgaggct ccactcgttc tgtttgtcgg ccggctggcc gagcagaagc gcgtgcatga
     1981 tcttttgaag gcgctcgatt tactgcaaca cgttcagcaa gatctgcgga gcctgatcgt
     2041 aggcgaaggg ccgttgcgcg attacctcga agacaccgcg cacgcctacg accttgacgg
     2101 ccggctgcgg tttctgggcc atcgggacga tgtgccgcgg ctgatggcgg cggccgatgt
     2161 gattgtcttg ccgagcgcct atgagggatt gcctaacgta gtgctcgagg cgatgcgctt
     2221 tggcaagccg gtagtcgcca ccgcggcgcc ggggacaacc gaggtggttg tcgacggcga
     2281 gaccggggtt ttggttcctg tgggcaacgt catgcttttg gcccgcgcga tccgcgacgt
     2341 cgtgcgcaac cccgttgagg ctgcccgcat gggcgaggca ggccgagctc gcgccgaatc
     2401 cctcttccgc gtcgacacta tggtcgagaa gttcgccgag ttgtatgaaa ctctggccca
     2461 atcgaagggg atcgtcgttt catgaatcgc ttctgggtcc gaccacgcgg attgcgttga
     2521 caatcgactc aacgcgtctg cactggctcc tccgctcggt taagtttcga ggtattcggg
     2581 ccgttggaac gaagtgactc gatcaacgag gtataggcga gcgtcaggtc gcgcctgctc
     2641 attaatccaa gcaagtgggt ggagtcgccg ggctcgacga ctgggatttc gtcggtattg
     2701 atttcggtca tccggcgcag ggcagtatga aggttgtctt cgggcgtgac cgagagcacg
     2761 ggacggtacg ccagatcgtc ggccagaacc agaggtcccc attctgagcc cacgagcgcc
     2821 agccgcaggt cgtggagggt gaaaatccct gtgagccgtc catttccgtc gacaaccggg
     2881 aagagggttt cagtcgactg ggcgaccagg tgaatcagct cgcgaaaggg catcgaagca
     2941 ggaatcgtcg cgatgcccac cgatcggatt ccgacgtcgc tcacagagag ctgctccaag
     3001 acgtcaacca cgaagtcgcc ctggtgggcc gggctatcga tgggggtgga aacctgctct
     3061 tcgtagattg tccagcggcg cgagatcgcc atgttcaggc cgcaagcgag cattaacggg
     3121 accagcaagc tgtaagagcc ggtcatttcg ctgaccatca cgatcgaggt cag
//
LOCUS       DATAQL010000690         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2972571, whole genome shotgun sequence.
ACCESSION   DATAQL010000690 DATAQL010000000
VERSION     DATAQL010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2972571"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VHS97_12060"
     CDS             <1..546
                     /locus_tag="VHS97_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448984.1"
                     /translation="GVPLVILSPSAPAGRVVESPVSLRDLPATVVDQLGLSAGSPFPG
                     RSLAAYWRSKPGQVPRGITTPALSEQADAIAFEPQPQTGHGHSGFQMSLVASGHHYFR
                     TGLGAEMLYDLVRDPSELINLIGSSYGDQTVGVFRNMLLEALSDEPGSIEVEEFYLKT
                     YRQRLKALVQESSSRQVAAGH"
     gene            complement(591..1142)
                     /locus_tag="VHS97_12065"
     CDS             complement(591..1142)
                     /locus_tag="VHS97_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3448985.1"
                     /translation="MAVSRGGVAGARFALPLLEAALAARPDDVTAWERMAHALYILGR
                     YDETLAAYRKALSMEPGRESAVAEAARVAVKLDQRQDAATYWQRAIAINPWRSDYHGE
                     LARVYFSDRDWQASANACLQALRLNSSWLKVRKLLVQCYLNLGNIEAARGELETVLGF
                     DPPDRTELLRSFAVQSRSGSRAP"
     gene            1209..2624
                     /locus_tag="VHS97_12070"
     CDS             1209..2624
                     /locus_tag="VHS97_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448986.1"
                     /translation="MAAVTASFAGIDRDWNPSAPMTEDTMIRRRGMRDVNAARLRHVT
                     AGAVIGAAAVQPFARWKAATPLSVTLETPIPVIRNLLGGGRKMVWIVARNAPEPTATG
                     AKAIALVHLFKLADKTVFGLVRRLNEHRPEAKKRQPGSIVFISPTNAHNPRITGQVAL
                     RTDVIPERGLQIGRIDDRHVLAVDHRGTGDVQFAWPMTALTADGVTLEDWQSILVSWA
                     CHRLDPVRMAEQAIGLDRPVEVIVDGFKTRRQTPLLLPRIPGKRGLEQKAIVVDQVGR
                     SLAAGANHELDLGLALGDNPALCIASRLLVKDVTVPMFDGVLEALAFEHSVAASLVTL
                     QYVVFADGCEGAAHRVLAEGKGTLRVAADTRRIPEILYLRAGVLIGRDRNFPTDAFRL
                     LAPPRNKDHPRAHHNDRSEKHEPLRSQSRRSGLRIDRALQLPVLVTGPSCLTPAQRTL
                     RGTNRKFVSDCTMTLAGPANI"
BASE COUNT          525 a          843 c          811 g          508 t
ORIGIN      
        1 ggcgtcccgc tggtgatcct ttccccgagc gccccggcgg gccgagtggt ggaaagtcct
       61 gtgagcctgc gcgacctgcc tgcaaccgtg gtcgaccagt tggggctttc ggccggctca
      121 ccgttcccgg gccgctcgtt ggcggcgtac tggcggtcta aacccgggca ggtgccccgc
      181 ggaatcacca ccccagcact ctcagagcag gcggacgcga tcgctttcga accgcagcct
      241 caaaccggcc acgggcacag cgggtttcag atgtcactcg tggcctcggg ccatcattac
      301 tttcggactg gtttgggagc cgagatgctc tacgatcttg tacgagatcc gtccgagctc
      361 atcaacctca tcggttcgtc ctacggcgac caaaccgtgg gagtctttcg aaacatgctc
      421 cttgaggcgc tgagcgatga acccggctcg attgaggtgg aagagttcta cttgaagact
      481 tacaggcaac ggctcaaggc cctcgttcaa gagagttcct cgcgacaagt cgccgccggc
      541 cattgaatcg tcatcgatct tgttgtttga actcgacaga cccgcgcgga tcacggggct
      601 ctgctgccgg acctcgactg cacggcaaat gagcgcagta actcggtccg gtcaggcgga
      661 tcgaagccaa ggaccgtctc aagctcgccg cgtgcggctt cgatgtttcc cagattgaga
      721 tagcactgta cgagcagctt cctgactttg agccaggagg agttgaggcg aagggcctgc
      781 aggcaggcgt tggctgacgc ttgccagtcg cggtcgctga aatagacacg cgccagctcg
      841 ccgtggtaat ccgaacgcca cggattgatg gcgatggcgc gctgccagta agtggctgcg
      901 tcctggcgtt gatccaactt cacggcgacg cgagccgcct cggccacggc ggattcccga
      961 cccggctcca tcgacaaggc cttccggtaa gcagcaagtg tttcgtcgta tcggccgagg
     1021 atgtagagcg cgtgggccat gcgctcccat gcagtcacat catccggccg cgccgccaga
     1081 gccgcctcga gcaacgggag tgcaaaccgc gcacccgcca ctccgccgcg ggacacggcc
     1141 actccgcggt ctcgctcaac cagggctcgc tcgtcgccat ccatccgctc gcgatggaaa
     1201 acgaccagat ggcggctgtc acggccagtt ttgccgggat tgaccgcgat tggaatccct
     1261 ccgcccccat gacggaggat acgatgattc gccgtcgcgg catgagggat gtcaacgctg
     1321 ctcgccttcg gcatgtgaca gctggtgcag tcatcggcgc tgctgcggtc caaccgttcg
     1381 ctcgctggaa ggctgcaacc ccgctcagcg tgacactcga gacaccgatc ccggtaatac
     1441 gcaaccttct cggcgggggc aggaaaatgg tgtggattgt ggcaagaaat gcacccgagc
     1501 cgaccgcgac tggcgcgaaa gcaattgctc tcgtgcatct gttcaaactg gccgacaaaa
     1561 cggtcttcgg cctggtccgg cggttgaacg aacaccgtcc agaagctaaa aaacggcagc
     1621 ccgggtcgat agtcttcatt tcgccgacca acgcgcacaa tccgagaatc accggccagg
     1681 tggcactgcg cacagacgtc atcccggagc gcgggctcca gatcggccgg attgacgatc
     1741 gtcatgtcct tgccgtcgac catcgtggga cgggcgacgt gcagttcgcc tggcccatga
     1801 cagcgctcac agccgatggc gtgaccctgg aagattggca gtcgatattg gttagttggg
     1861 cctgccatcg gctcgacccg gttcgcatgg cagaacaggc aattgggctg gatcggccgg
     1921 tcgaagtgat agttgatggc ttcaaaacca ggcgacagac cccattgctg cttccgagaa
     1981 taccaggtaa gcggggactc gaacagaaag ccatcgtggt cgaccaggta ggaagatccc
     2041 tggcggccgg agccaatcac gaactggacc tcggcctcgc tcttggcgac aatccggccc
     2101 tatgcatcgc gtcgcgtctc ctggtgaaag acgtgaccgt cccgatgttc gatggagtac
     2161 tggaggcctt ggctttcgaa catagtgtgg ccgcctccct cgtcaccctt cagtacgtag
     2221 ttttcgccga tgggtgcgag ggagcggccc atcgggtgct tgccgaaggc aagggcactt
     2281 tgcgcgtggc agccgacaca cgccgaatcc ccgagatact ttacctccgg gcgggtgttc
     2341 tcatagggcg agaccggaac tttccgacgg acgctttcag acttctggct ccgccacgaa
     2401 acaaagacca tccacgagcc caccacaacg accgcagcga aaagcatgag ccgttgcgtt
     2461 cgcaatctcg acgatcgggt ttgagaatcg atcgcgcgct tcaattgcct gtcctggtca
     2521 ctggcccatc ctgcctcaca ccggctcaac gaacgttgag gggaacgaac cgcaagttcg
     2581 tctctgattg taccatgacc ctcgccgggc ccgcgaacat atgatagttt agaggccgga
     2641 ttgagccctg atcgcccttg aacgctcaat tttggccccc gagcgtt
//
LOCUS       DATAQL010000691         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2363071, whole genome shotgun sequence.
ACCESSION   DATAQL010000691 DATAQL010000000
VERSION     DATAQL010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363071"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VHS97_12075"
     CDS             complement(<1..974)
                     /locus_tag="VHS97_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX3448987.1"
                     /translation="MESGSDKSSVPRMRQRVCNGLRRLAVIALSAIIIAGLIGQVVRD
                     RSVVFAVLMYLPLLPAGLAAIALDLVARGRAIPRARFGLALLGIVACTLPLVSMIALG
                     PAIVHRVDTPEVTLLHWNVQWGGGPFRSQQTWAAQRSEIFRRDPDIIVLCELPPADWL
                     ARLADDMGSGASTVYLEHDASSRYRYRMAVCSRWPVRLEERVSLPGGAGMSVTADVRG
                     RRLRLLCVDGQSNPFRSRLPFLVAITDLCRAASDAGRPFDVIVGDFNTPSRSLGFDSL
                     MDQGYTLASRSSAGLRGTFPSWFPLYDIDHVWLGPGLIARACRLYNGPA"
     gene            complement(1071..2099)
                     /locus_tag="VHS97_12080"
     CDS             complement(1071..2099)
                     /locus_tag="VHS97_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448988.1"
                     /translation="MTSTTILLLLAATAAEPADIPLPTELFEAPKYGLVTRIPKSWQV
                     AVHEQEDRIFVAIIPQKELDRPGVAACELALAPETLDDYRTRIDTSSKKADPDRGTLA
                     SNRVIKDAQGERLETIWEFHPPAGGFWREVKVRTIANRQLYTFILNVEDAVYARVRPA
                     FDALVATTRFSPPNTGADLLAKSSNRWIQREYKFALDLPEGWAPVLAPSEVALLFANG
                     APHGVWSDNLLVVAYPHKDLDLHEVVKQLPEELRREDPACEVISCKVVAGEKAEALET
                     VVRTNRGPFSMTVIERRFRGGRLDYEVKYTVESKRFDQLVPKFQESFKSFRELPGEVP
                     TSATRKAT"
     gene            complement(2114..4015)
                     /locus_tag="VHS97_12085"
     CDS             complement(2114..4015)
                     /locus_tag="VHS97_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448989.1"
                     /translation="MSAGPATADDFLTISPRIRVMPIIHGSGDFAIQVREELLSRPYD
                     CVAVPLPPSFQEDVEAAVEQLPTISVVVQVDAEMEDDERAGESGFSYVPIDPCQGVIT
                     ALRTARGERIARAYIDLETPRFEPVTGTFPDPYALKRVKPERFAAAVLAAVPPPVAGQ
                     NTERIAWMAARLRELEARRRSILLVCSILDWPWIRDAYQRRLEPPEPASFFSPITTYR
                     VDPGTLIFMLGELPYITGLYERGRRELTADDNLSVDGVKEMVLDARERLRQRHPRIAE
                     RVTPQMLSVLFRYIRNLSLLDRRLTPDLYTLIVAAQQTAGDDFAIALAETARSYPYSD
                     VDSDDMQDTTGFHESGQARMGIHQAELPVWGTGPMVSRLPGQAISWRTCELRPRPHKE
                     AQKEWRQRWDPFGMCSWPPEDDRIESFHRHVREQAKAMLGADLARTEKFTTSVRDGID
                     IRETLRNWHKGDLYVKVVPPSRGSLEVVVFLFELPADPATYVNRTTWYAEHAQESTLA
                     FYATDPMRNLVGPGIAQAQYGGALFIFPPRPIPDVWVDKRLDFTDTLEERLLAAAFLH
                     SHDRHVAVVSPKIPPAAWRRLARKFGKKIVHLPLKRFSGQLLERLRTFHVLNGKYVRS
                     YGADYIRDM"
     gene            4203..>4709
                     /locus_tag="VHS97_12090"
     CDS             4203..>4709
                     /locus_tag="VHS97_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEX3448990.1"
                     /translation="MMVAFRETVRRLFPPLKIRARLWPIMLLLAGYGVLVSYAITLEH
                     LPPIEWGAESTVVNGLALSFLIGFRNNHAYDRWWEARKLWGQLINENRNLCLKVQSLA
                     GIDAADRDALGQLIITFADSLKDHLRRREGRDSTPAEFETDVQWRHQPSLAAHGILEL
                     IVRWQREGH"
BASE COUNT          955 a         1428 c         1448 g          878 t
ORIGIN      
        1 gccggcccgt tgtagagtcg gcacgcgcgc gcaatcagtc ccggaccgag ccagacatga
       61 tcgatgtcgt agagagggaa ccacgatgga aacgtcccgc gcaaaccggc agacgaccgg
      121 ctcgctagtg tataaccctg atccatcaac gagtcgaacc ctaggcttcg gctgggagta
      181 ttgaaatcgc ccacgatcac gtcgaaagga cgaccagcgt ctgacgcggc gcggcaaaga
      241 tcggtgattg ccaccagaaa cgggaggcgc gatcgaaacg gattgctttg gccatcgacg
      301 caaaggagcc gcaggcggcg gccccgtacg tcggccgtca cgctcatgcc ggctccgccg
      361 ggcaaggaaa ctcgctcttc cagccgaacg ggccagcgcg aacagaccgc catacgatac
      421 cggtaacgac tgcttgcatc atgctcgaga taaacagtcg acgctccgga tcccatgtca
      481 tcggccaacc gtgcgagcca gtcggctggc gggagctcgc acaggacgat gatgtcgggg
      541 tcacgtctga aaatttcaga ccgctgtgca gcccacgttt gctggctgcg gaacggcccg
      601 cctccccact ggacgttcca atggagcaaa gtgacctcgg gagtgtcgac gcggtgtact
      661 atcgccggtc ccaacgcgat catcgagacc aatggcaatg tgcaggcgac aatgccgagg
      721 agagccaggc caaagcgtgc ccgcggaatg gcgcgacccc gcgcgacgag gtcaagcgcg
      781 atcgccgcaa gccctgccgg caagagcggt agatacatca agacggcaaa taccaccgag
      841 cgatcgcgga ccacctgacc gatcagcccc gcgatgatga tggcggacag ggcaatcacc
      901 gcgagacgac gcagcccgtt gcaaacgcgc tggcgcatcc gcgggacgga cgatttatcc
      961 gagccactct ccattgcttt tcccgcagaa agccttagcc ggcgacacgt tccgaacgca
     1021 tgcggaccgg ccgttgcaaa gggacccgaa ttgtctctgg accacgccac tcaggtcgcc
     1081 ttccttgtag ccgacgttgg aacctcgccc ggaagttccc ggaagctctt gaaactctcc
     1141 tgaaacttgg gaacgagctg gtcgaatcgc ttggactcaa cggtgtactt cacctcgtag
     1201 tcgagccggc cgccacgaaa acgccgctcg atgacagtca tcgaaaaagg cccgcgattg
     1261 gtgcgtacca cagtctcgag tgcttcggcc ttctcgcccg ccacaacctt gcaagaaatc
     1321 acctcgcagg cgggatcctc acggcgcagc tcttcgggca gttgcttgac cacctcgtgc
     1381 agatcgaggt ccttgtgggg ataagccacg accaggaggt tatccgacca gactccgtgc
     1441 ggtgcaccgt tggcaaacag aagcgccact tcactgggcg cgagcaccgg agcccatcct
     1501 tcagggagat ccagtgcaaa cttgtactca cgttgaatcc accgattcga cgacttcgct
     1561 agcaggtcgg cgcccgtgtt ggggggcgaa aagcgggtgg tggcgaccag agcgtcaaat
     1621 gcggggcgaa cccgggcata gaccgcgtct tcgacattca ggatgaacgt gtagagctga
     1681 cggttggcga tggtacgcac cttgacttcg cgccagaaac cgcctgctgg tgggtgaaat
     1741 tcccagattg tctcgagccg ttcgccctgg gcatctttga tgactcgatt agaggcgagc
     1801 gtgccgcggt cggggtcggc cttttttgag ctggtgtcga ttcgggtacg gtaatcatcg
     1861 agagtttcgg gcgcaagggc cagctcgcag gcggccacgc ccggacgatc aagttctttc
     1921 tggggaatga tcgccacgaa gattcgatct tcttgttcgt gtactgcgac ctgccagctc
     1981 ttgggaatgc gcgtcaccag gccgtacttg ggtgcctcga acagctcggt gggaagcgga
     2041 atgtccgcgg gctcggcggc agtcgcggct agcaacagaa gtatggtggt gctcgtcatg
     2101 gtaactgctc ccatcacata tcacgaatgt aatccgcccc ataagaccgg acgtacttgc
     2161 cgttgagcac gtgaaaagtc cgcagccgtt cgagaagctg accgctgaat cgtttgagcg
     2221 gcaggtggac gattttcttg ccgaatttgc gagcgagtct tcgccaagcg gcaggaggaa
     2281 tcttcgggct gacgaccgcg acgtgccggt cgtggctgtg tagaaaggcc gcggccagca
     2341 accgctcttc gagggtgtcc gtgaaatcaa gccgcttgtc gacccacacg tcggggatcg
     2401 gccgcggcgg aaagataaaa agggcacccc cgtactgggc ctgggcgatc cctggcccga
     2461 cgaggtttct catggggtct gtggcataga aggccagtgt tgattcctgc gcatgttccg
     2521 cgtaccaggt tgtgcgattg acgtaagtcg cggggtcggc cggcagctcg aacagaaaga
     2581 ccaccacctc gagcgatccc cggctcggag gcacgacctt cacatacagg tctcccttgt
     2641 gccagttcct cagggtttcc cgaatatcga tgccgtcgcg cacgctggtc gtgaacttct
     2701 cggtacgggc caggtccgcg cccagcatgg ccttggcctg ctcgcgcacg tgacggtgaa
     2761 agctctcgat ccggtcgtcc tcgggtggcc acgagcacat gccaaaagga tcccaccgtt
     2821 gccgccactc tttttgcgct tccttgtgcg gacgcggccg cagctcgcac gttcgccacg
     2881 agatcgcctg acccggcaac ctgctcacca tcggcccggt gccccagaca gggagctccg
     2941 cctgatgtat tcccatccgt gcctggcccg actcgtgaaa accggtcgta tcctgcatgt
     3001 catcgctgtc aacgtcgcta tagggatacg accgtgcggt ctctgccagc gcgatcgcga
     3061 agtcgtcgcc ggccgtctgc tgggcagcga cgatcagcgt gtaaaggtcg ggagtgagcc
     3121 ggcgatcgag aagcgagagg ttgcgaatgt aacgaaacaa caccgacagc atctggggcg
     3181 tgacccgctc ggcgattctc gggtgtcttt gccgcaatcg ctcccgagca tcgagtacca
     3241 tttccttgac gccgtcgacc gaaaggttgt cgtcggccgt cagctcgcgc cggccccgtt
     3301 catacaggcc ggtgatataa ggaagctcgc cgagcataaa aatcaaggta cccggatcca
     3361 cgcgataggt cgtgatcgga gagaaaaacg aggcgggctc ggggggctcc agacgccgct
     3421 ggtaggcatc gcgaatccag ggccagtcga ggatagaaca gacaaggagg atcgaacgac
     3481 ggcgagcctc cagctcgcgg agtcgggctg ccatccaagc gattcgctct gtgttttgac
     3541 cggcaacggg gggcgggacg gccgccaaca cggcggcggc gaaacgctcg ggcttgaccc
     3601 tcttgagcgc ataaggatcg ggaaacgtcc ccgtcacggg ctcaaaccga ggcgtttcga
     3661 ggtcgatata tgcgcgcgcg attcgctcgc cgcgcgccgt tcgcaaggcg gtaatcactc
     3721 cctggcacgg gtcgatcggg acgtaactga agcccgattc gccggctctc tcgtcgtctt
     3781 ccatttcggc gtcgacctgg acgacgactg aaatcgtcgg aagttgttcg acggccgcct
     3841 cgacgtcttc ttgaaacgac ggaggcaacg gcaccgcgac gcaatcataa ggccggctca
     3901 gcaactcttc gcggacctga atggcaaaat cacctgaacc gtgaatgatg ggcatgaccc
     3961 tgatgcgcgg actgatcgtc aggaaatcgt cggcagtcgc cggtcctgcg ctcatcgatc
     4021 actccgcttc tcgggctcgg cctttccgtc tcgggcttcc gatctataag atgcccaagc
     4081 cggtattgaa agttcggcag attcatcgta cgtgttcaga ccgtcctata ctcttctagt
     4141 gtaacgttgg actgccccaa aaggggacgg gtgctggctc aatccgttgc gaggcaagct
     4201 tgatgatggt cgcgttccgg gaaaccgtgc gccgcttatt tcccccgttg aaaatcaggg
     4261 cgaggctctg gccgatcatg ctccttctcg ccggctacgg tgtgctggtg agctatgcca
     4321 ttacgctcga gcacctacct cctattgaat ggggagcaga gtcgaccgtc gtgaacgggc
     4381 tggccctgag ctttttgatc ggttttcgca ataaccacgc ctacgaccga tggtgggagg
     4441 cacgcaaact gtggggtcag ctcatcaacg aaaaccgcaa tctttgcctg aaggtgcagt
     4501 cgttggccgg gatcgacgct gccgaccgcg atgccctggg tcaactgatc atcacgttcg
     4561 cggattccct caaagaccac ctccgcaggc gcgagggaag agactcgacg cctgccgagt
     4621 ttgaaaccga tgtacagtgg cggcaccagc cgagcttggc tgcgcacggc attctcgagt
     4681 tgatcgttcg ctggcagcgg gaaggtcac
//
LOCUS       DATAQL010000692         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2513301, whole genome shotgun sequence.
ACCESSION   DATAQL010000692 DATAQL010000000
VERSION     DATAQL010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513301"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            637..939
                     /locus_tag="VHS97_12095"
     CDS             637..939
                     /locus_tag="VHS97_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448991.1"
                     /translation="MPSPSRPKRYPDSGRGRPARWNWIGMTVPLLVAMLAPISAQAGE
                     VVLSDVTLTARATFSSQRVTPEKSDFDVKTAPPDQLLQAAQALVIEGLPFPNQPNS"
     gene            988..1659
                     /locus_tag="VHS97_12100"
     CDS             988..1659
                     /locus_tag="VHS97_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3448992.1"
                     /translation="MGVNGFHFENSLPPNRYFASGTWTQTLTNDSTVTQVSSATVSVP
                     APTIRFFGVGNSFPPGANPDLDATASVDIRLTTKLTHADGSVVETVLFDYGMHTLRAP
                     IVGVLLAVPSDDALGNLSRFDEPDGSFGFRLLPVLKNFSYGAVRPGESLEFGYDYFAT
                     ASTGFGETAVFAAIGDPFDLTTSGSFSVQVSSIPEPGTLTLLGLGLAGLAAIWVRKWL
                     KPQSV"
     gene            complement(1947..2141)
                     /locus_tag="VHS97_12105"
                     /pseudo
     CDS             complement(1947..2141)
                     /locus_tag="VHS97_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
BASE COUNT          451 a          744 c          705 g          485 t
ORIGIN      
        1 cttcaccgcc taacgccacg gctcagcggc cccgcgccgc agagcatgga caacaccgga
       61 cgacttgtcg cgggctccgc tggagcgcga ctttaatctc ttaaacccca tcgggtaccg
      121 ccatcggcgg ggaacatttc cccggttgcc tttcagctcg cctaatcttg atgttccttg
      181 gaagtcgact agaacatccg ccagagggaa acggctggcg gcgcaccgcc tgtctgtcct
      241 atcggcgatg attcggtatg aaaacgcgcg tcgcgacgat gccggcgcgt aggctcagat
      301 gttgggcaac gatcaagctg agtgaacgtt ttgagcatgg aaggcggcta cggccgctgc
      361 gccggtcaaa ataatatctg agatcgtgtg accttttcga cgcgattgca ggaatagagt
      421 tgcggcgaca gtttgcgccg tctttgtttg gacgatgcga tcccgatcaa agactgcgac
      481 gtcttaccgt ttcactggct tagccgtggt cggatctatc ctgtcagtcg gatttgctgt
      541 cacctccggc acgctgggtg agcttgggta gaggagcggc gcacacgcat caccttcggc
      601 gtagctcatc gtgtcgtcaa ggagaggggg ctcgaaatgc catcaccaag tcgaccaaaa
      661 cgctaccccg actcaggccg cggtcggcct gcacgctgga actggatcgg aatgaccgtt
      721 cctttgcttg tcgccatgct ggcgccgatt tcggcgcagg cgggcgaggt tgttctatcc
      781 gacgtgacac tcaccgcccg tgccaccttc agctcgcaac gggtgacgcc cgagaaaagt
      841 gactttgatg tgaagaccgc cccgcccgac cagcttcttc aagcagcgca agccctggtc
      901 atcgaggggc tcccctttcc caatcagccc aacagctaga acgcaggctt cgcgtcgtct
      961 gcggcgacgg tcggcggcgt ctttggcgtg ggcgtgaacg gattccattt tgagaattcc
     1021 cttcccccga accgctactt tgcgtcgggg acatggacac agaccctgac caatgactcc
     1081 acagtcaccc aggtgtcgag cgcgaccgtc tccgtcccgg caccgaccat acgattcttc
     1141 ggcgtgggca attccttccc cccgggcgcg aacccggacc tggatgccac ggcgagtgtc
     1201 gacatcaggc taacgaccaa actcacccac gcagatggca gtgtggtgga gaccgttctc
     1261 ttcgactacg gtatgcacac tctgcgggct cccatcgtcg gcgtgctcct tgccgtcccg
     1321 agcgacgacg cgctggggaa cctgtcgcgc tttgacgagc ccgacggctc attcggcttc
     1381 cggttgttgc ctgtgctcaa gaatttctct tatggcgcag ttcgacccgg cgagagtttg
     1441 gagttcggtt acgactattt cgccacggcc agcacgggct tcggcgaaac cgccgtcttt
     1501 gccgccatcg gcgatccgtt cgacttgacc acgagcggca gcttcagcgt tcaggtgagc
     1561 agcatccctg aacccggcac actcacgttg ttaggccttg ggcttgctgg gttggcggcg
     1621 atctgggtgc ggaaatggtt gaaaccacag tccgtctaat attggcatcg gtgaccatgc
     1681 cgcgatcacc gacgaagacg aggcgattga agccgaatcg ctgatgcaag tcgcggatgg
     1741 ttctctgcac cgtgccatgg tcgagctcat tgccggccca gacgtgatgg gcgatcggtc
     1801 agccagccac catcaccgcg ccgacgatcg acagggcgcc gtggcgacac gccccgagcg
     1861 ccgaccacgg gaccaagacc gaagtggggc ggcaggctgc cgccctgtct ttcccttcgg
     1921 gattggtacg aacctttccg gtgggatcac gccccgatcg ccttgccgac tgcttcaacg
     1981 agttcgtctt cgtcgggaaa cgtgcccgtc ttgagcttgg aatagagctc cgcgccgccc
     2041 actgtaagct cgaaacagcc tcctccggag ggaatcatga cgaaatgact gatggcgcgc
     2101 ttgtgacgag ttaagagctt ggccgtcaaa ctgacggctt gaggttcata gtttcaaagg
     2161 gcacagtcct cgagcgtaac gtccacgggg gctctcctta tcctgttcgt cggccgccag
     2221 ccataccgag ttgcctggca gcatcgttct catcggtcat catcgactca tttcagctgg
     2281 ctcgcaagat ggccgcaagg acacgcacgg cgtggcaaga tccagacagc gcaacaacat
     2341 gcgattcgcc gcaggttcgc gccgtgcctc agacagacaa ggcgg
//
LOCUS       DATAQL010000693         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4194750, whole genome shotgun sequence.
ACCESSION   DATAQL010000693 DATAQL010000000
VERSION     DATAQL010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4194750"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="VHS97_12110"
     CDS             <1..1920
                     /locus_tag="VHS97_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX3448993.1"
                     /translation="DRARLVANVDWLERAQHKDPRRESWPGNWTYSSQRAQAGDNSNT
                     QYALLGLNAASEAGIAVRPDVWELARAYFEHFQNRDGGWGYTPRHKQSTASMTCAGVS
                     SLIISGSRQFHSAEYLHGDTIHHCGDGGLDPFLKRGIDWLATHFDVRQNLGHGQQYKF
                     YYLYGLERVGRLAAVRFLGQYDWYRLGAQELVRQQNKLSGFWTGGGESQIVATSFALL
                     FLAKGRAPVLVNKLRYLPGNDWNHDADDVRNLVGVVSRDWKSLLTWQVVDPSAATLQD
                     MLQAPVVFFNGHIAPEFPPGAKRNLRDFVEQGGFVFAEACCGSHEFDRGFKQLIKDVF
                     PEPDYRLRPLPPDHPVWRAKHLLSPEIHPLWGIEHGCRTVLIYSPQDLSCYWNQLERS
                     PANPAVIKAIRVGQNVIDYATGREMPDDKLTMRETHEFKKDIPRRGALRIAKLKHAGE
                     WNIAPQAIPNLMETLRRPPINFDVTITQKDLFPNDPSLIYFPLIYVHGRAALSLENED
                     LESLRRHLDPGSGTLFADAACGSAAFDESFRRFAALLFPDKPLVSIPAGDELYSRKIG
                     FDLSLVEYTQAAGGGHGPPKLEGVKINDHWAIIYSKYDIGCALERHTGIDCLGYTHAS
                     ATKIAANIVIYATLP"
     gene            2031..2636
                     /locus_tag="VHS97_12115"
     CDS             2031..2636
                     /locus_tag="VHS97_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3448994.1"
                     /translation="MPETTTNEEYAEFIRRIRQGDEQAAEELVRRYETEIRLEVRGWL
                     RLRNPGLKRIFDSMDICQSVMASFFARAAVGDFDLEEPPQLIRLLVGMARKKVAEQSR
                     HHHRQRRDVRRVGEGDLDNAPARSSAQTPSRLASGRELLQKFRELLSDDERRIADLRA
                     RGTDWAGVAAELGGTPDGRRKQLARAVSRIELDLGLDSVLD"
     gene            2765..>3127
                     /locus_tag="VHS97_12120"
     CDS             2765..>3127
                     /locus_tag="VHS97_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448995.1"
                     /translation="MIEYASGNASPSDPWHRLERGDHCSQDELVEILCADQANRWRGG
                     ERIPAEAYFARHPNIAGDSEGAFELVYGEYLVRELLGECPKFDEFLWRFPRFADRLHR
                     QLNLHQALAQEEATQKPDS"
BASE COUNT          642 a          948 c          922 g          615 t
ORIGIN      
        1 gaccgcgccc ggctggtcgc caatgtcgac tggttggagc gcgcccaaca caaagacccg
       61 cgccgcgagt cttggccggg aaactggact tattcatcgc agcgggctca ggccggcgat
      121 aactcgaata cccagtatgc cctcctgggc ttgaatgcgg ccagtgaagc cggcattgcc
      181 gtgcgtcctg acgtctggga gctcgcccgg gcctattttg agcacttcca gaatcgcgac
      241 ggcggctggg gctacacgcc cagacacaag caatcgaccg ccagcatgac gtgtgcgggc
      301 gtttcgagct tgattatcag tggctcgcgc caatttcaca gtgccgaata ccttcacggc
      361 gacacgatcc atcattgcgg agacggggga ctcgaccctt tcttgaagcg aggcatcgat
      421 tggcttgcca cccattttga cgttcgccag aatctcggcc acgggcaaca atataagttt
      481 tattacctct atggactcga aagggtcggg cggctcgccg ccgtacgttt cttgggacag
      541 tacgactggt atcgcctggg cgcccaagag ctagtccgtc agcagaacaa gctctccgga
      601 ttctggaccg gaggaggcga gagccaaatt gtcgcgacca gctttgcgct cttattcctg
      661 gccaaagggc gcgcgccggt actggtcaac aaacttcgat atcttcccgg caatgactgg
      721 aatcacgatg ccgacgatgt ccgtaatctg gtcggtgtcg tgtcgcgtga ctggaaaagc
      781 ttgctgacgt ggcaggttgt cgacccgagc gcggcaactc ttcaggatat gctccaagct
      841 ccagttgttt tctttaacgg ccacattgcg cccgagtttc cacccggagc caaacgcaat
      901 cttcgcgact tcgtcgagca agggggattt gttttcgccg aggcctgttg cggtagccac
      961 gaatttgatc gcggattcaa acaattgatc aaggatgtgt ttcccgagcc agactatcgg
     1021 ctccgtccgc taccgcccga tcatccggtc tggcgtgcca agcacttgtt gtctcccgag
     1081 attcacccgc tctggggtat cgagcacggc tgccgcactg tgttgatata ctcaccacaa
     1141 gatctctctt gttactggaa tcagctcgaa cgtagccccg cgaatcccgc ggtcatcaag
     1201 gcgatccgag tcggtcaaaa cgtcatcgac tacgccacgg gccgcgagat gccggacgac
     1261 aagctcacga tgcgggagac tcacgagttc aagaaagata ttccccgacg cggagccctg
     1321 cgaatcgcca agctcaagca tgccggtgaa tggaacatcg ctccccaggc gattcccaac
     1381 ctgatggaga cgctgcgtcg tccgccgatt aacttcgacg tcacgatcac ccagaaagac
     1441 ctgtttccga acgacccgag tctgatttat ttcccgctca tctacgtcca cggccgcgcg
     1501 gcccttagcc tcgagaacga agacctcgag tcactgaggc gacatctcga ccccggaagc
     1561 ggtacgctct ttgccgatgc ggcgtgcggc agtgcggcat tcgatgaatc gtttcgccgc
     1621 ttcgccgctc ttctctttcc ggacaagccg ctggtctcca tacctgccgg cgacgaattg
     1681 tattcgagaa agatcggctt cgatctgtcc ctagtcgagt acacccaggc agccggcggc
     1741 ggccacggcc ctcctaagct cgagggggtc aagatcaacg accattgggc gattatctat
     1801 tcgaaatatg acatcggctg cgcactggaa cggcacacgg gcatcgactg cctcggctac
     1861 actcacgcga gcgccacgaa aatcgctgcc aatatcgtca tctacgcaac attgccatag
     1921 tcgcggcggc tcgcgatgaa cttggcgcgg cgtccttctg tcggtggcgg gaccgggcta
     1981 taatctgaat cagttctaac ggccatgaca ggtcgcggtc ggtgggcacc atgcctgaaa
     2041 cgacgacgaa cgaagaatat gccgagttta tccgccggat ccgccaaggg gacgagcaag
     2101 cggcggaaga gttggtgcgt cgttacgaaa ctgagatccg gctggaagta cgaggatggt
     2161 tgcggctgcg caatccggga ttgaaacgca tcttcgactc gatggacatc tgccaatcgg
     2221 tcatggccag cttcttcgcg cgggcagcgg ttggcgactt cgacctggag gaaccgccgc
     2281 agttgatccg gctcttggtc ggcatggcgc gtaagaaggt cgcggaacaa tcccgccatc
     2341 accaccggca gcgccgtgac gtgcgccgcg tcggcgaggg agatctcgat aacgcgcctg
     2401 cccgctcgtc ggcgcaaaca ccgagccgcc tggcctccgg ccgcgagctg ctgcagaagt
     2461 tccgcgaact tctctcagat gacgaacgac gcatcgccga tttgagggcg cgagggactg
     2521 attgggcggg agttgccgcc gagctaggcg gaacgccgga tggccgtcgc aaacaactcg
     2581 cgcgagctgt aagccgaatc gagctggacc tggggctcga ctcggtactg gactaaaccg
     2641 atttcccgcc agcattgaga ttcgtccgtc cggcagaggc gccgacggca atgattcgaa
     2701 caggcaagac gatatgtgcc tgactttgcc actcttgctg tcgcattgat agggccaccg
     2761 agcgatgatc gaatacgcca gcggcaatgc gtcgccgtcc gacccttggc atcgactgga
     2821 gcggggagac cattgctccc aggatgaact ggtcgagatc ctgtgcgccg atcaggccaa
     2881 tcggtggcgc ggcggggaac gaattcccgc cgaggcatac ttcgcgcggc acccgaacat
     2941 cgccggcgat agtgaggggg cctttgagct cgtctacggc gagtacctgg tgcgggaatt
     3001 gctgggagag tgccccaagt tcgatgagtt cctctggaga ttcccccgct ttgccgatcg
     3061 gctgcaccgc cagctaaatc tccatcaggc gctcgctcaa gaggaagcaa cccaaaagcc
     3121 cgattct
//
LOCUS       DATAQL010000694         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1752278, whole genome shotgun sequence.
ACCESSION   DATAQL010000694 DATAQL010000000
VERSION     DATAQL010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752278"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VHS97_12125"
     CDS             <1..661
                     /locus_tag="VHS97_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3448996.1"
                     /translation="TPKITSQSPLLESFDYEIAGLLAKELGVRLRVFWSISAHDSYPS
                     KLSSKGLCDVILGVMPDDRFAQRVLYSRPYYRATYQRVIGAGTRSTFDGDPWAIEEGV
                     AVRGLTGRAVRFFPSTESILEAVATDRVSTGYVISTRGHWLAHERWPEKLTFLPATET
                     TDSFPICAAVRKPDGDLKEAIDQAWDKLDGSSQFGEVFTRWHIPQERVAVAGTRKGPG
                     P"
     gene            658..1581
                     /locus_tag="VHS97_12130"
     CDS             658..1581
                     /locus_tag="VHS97_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3448997.1"
                     /translation="MTSPKPVREFRRPGLLAASLIVGTALLALAGRSHAFRVRPAPIA
                     LEEAIQKHDGTGVQNTTAAKTAATPPTQDPNALAEGQALFRGLCTGCHGGTGRGGKGP
                     DLTDNRWIHGSSDQDIARCIHDGVPKTTMKKLGDALKDDQIKKVVAYIRSLARSPTEG
                     NWKPYIAGDPQAGRTLFFDPRGKALCAKCHSVAGDGGRIGPPLDRIASRRSAEFLMES
                     ILQPSKEIAPEYEAVIVATKDGHVITGLRVNETNFSIQLREENGRFHSFSKRDLDEVK
                     SMAKSLMPENLSEVLTVKELHDLLAYLMSLE"
     gene            complement(1729..>2808)
                     /locus_tag="VHS97_12135"
     CDS             complement(1729..>2808)
                     /locus_tag="VHS97_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3448998.1"
                     /translation="GGPSAGDILRSIDCGDESALAAARAQFYSRPIDVERSPALRLLL
                     AHRPGGDSLLLSANHAVTDGVGALRFLHSVARAYCGRPDPVPAVDPLAARNLQAQFSH
                     TVPHRPRNAERAKSPSGPRSLLVPKGADRESGYGFLPSALSADQARGLDPRRLVPTAT
                     VNDLLLASLHRAVAAWNADHNRPCHTISVLMPVNLRPPEWRNEVVGNLTLAGSTLSTP
                     DQRSTAESLLATMTAQTQRIKAGDDFAALLEMPLWVRKLLLILVLTKGARSMDTAVLS
                     NLGRIDDVPDFGTEAAEVTEFWFSPPVVMPMGLAIGTASLNGRLHLSMRYRRALFDDR
                     AALRFWELFLNSLTELTVVKRADVT"
BASE COUNT          569 a          842 c          918 g          479 t
ORIGIN      
        1 cacccccaag atcacgtcgc agagcccttt gctcgagagc tttgactacg agatcgcggg
       61 tctgctcgcc aaggagctgg gagttcggct ccgagttttc tggagcatct ccgcgcacga
      121 ttcctatccg tcaaagctct cgagcaaggg gctctgtgac gtgatcttgg gggtgatgcc
      181 cgacgatcga ttcgcccagc gggtcttgta ttcgcggcct tactaccgcg cgacgtacca
      241 gcgggtcatc ggtgccggaa cccgttcgac tttcgacgga gatccctggg cgatcgagga
      301 aggggtcgcg gtgcgagggc taacagggcg cgcggtacgg ttttttccga gcactgaatc
      361 gattctagag gcggtcgcga ccgatcgggt ctcgacgggg tacgtgatct ccacgcgagg
      421 gcattggctt gcgcatgagc ggtggccgga gaagctcacc ttccttcccg caacggagac
      481 tacggacagc tttcccatct gcgcggcggt gcgcaagccg gatggcgacc tcaaagaagc
      541 cattgatcaa gcgtgggaca agctagacgg atcgagccaa tttggcgagg ttttcacccg
      601 ctggcacatt ccccaagagc gtgtcgcggt cgccggaacc cgaaaaggac ctggaccgtg
      661 acaagtccca aaccagtgcg cgaattccgt cgaccgggct tgttggccgc cagcttgata
      721 gtcggaactg cgttattggc tttggccggc cgcagccacg cgttccgagt gcgcccggcg
      781 ccgatcgcgc tagaagaagc gatacaaaaa cacgatggca cgggcgtaca gaacacgaca
      841 gccgccaaaa cggccgccac gccccccacg caagacccca atgccttggc ggaaggccag
      901 gccctgtttc ggggcctgtg caccggatgt cacggcggga ccggccgcgg cggcaaaggc
      961 cctgacttga ccgacaatcg ttggattcac ggcagtagtg accaagacat cgcgcgctgc
     1021 attcatgacg gcgtgcccaa gaccacgatg aaaaaactgg gcgacgcgct caaggatgac
     1081 cagatcaaga aggtggtcgc ctacattcgg agcctggcac gctcgccgac ggaggggaat
     1141 tggaaaccct acatcgccgg cgacccccag gcggggcgga cgctcttctt cgatccgcgg
     1201 ggaaaggcac tatgtgcaaa atgtcatagc gtcgcaggcg atggtggacg catcgggccg
     1261 ccgctagatc ggatcgcgag ccggcgctcg gccgagtttt tgatggagtc gatcttgcag
     1321 ccatcgaagg agatcgcgcc ggaatacgag gccgtgatcg tggccacgaa agacggtcat
     1381 gtgattacag gacttcgagt caacgaaacc aacttcagca tccagcttcg cgaagagaac
     1441 ggcaggtttc attccttttc gaagagagat ctggatgagg tcaagagcat ggcgaaatcg
     1501 ctcatgccgg agaacctcag cgaggtgctg accgtcaagg agctgcacga tctcctcgcg
     1561 tatttgatga gcttggaatg aatgtcgaga ggcctgaaac gagcggtggg atcgttgatg
     1621 gatccacctc ttatgatcgt cggcggtccg aaggcgagac ccgtactaca gcggtcacca
     1681 gccgcaaggc caccgctatg ctgattcact ctgatatcca acatttcctc acgtcacatc
     1741 agctcgcttg accacagtca gttccgtcag gctgttgagg aacagttccc agaagcgcag
     1801 ggccgctcga tcgtcgaaga gcgcgcgccg atagcgcatg ctcaggtgga ggcgaccatt
     1861 caggctggcc gtcccgatcg cgagacccat gggcatcacg accggcgggg agaaccagaa
     1921 ctccgtgacc tccgcggctt cggtcccgaa gtccgggaca tcgtcaattc gccccaggtt
     1981 ggacaagacc gccgtgtcca tcgatcgagc acctttggtc aacaccagga tcaggagcag
     2041 cttacggacc cagaggggca tctcgagaag ggccgcgaag tcgtcaccgg ccttgatccg
     2101 ttgcgtctgg gcggtcatcg tcgccaaaag cgattcggcc gtcgagcgct ggtctggggt
     2161 actaagggtt gagcctgcaa gggtcagatt cccgacaacc tcattgcgcc actccggagg
     2221 cctcaggttg accggcatca ggaccgagat ggtgtggcac ggccggttgt ggtcggcatt
     2281 ccatgcggcg accgcccgat ggagcgaagc gagcaggaga tcattgaccg tcgcggtggg
     2341 cacgaggcgc cgcggatcca ggccccgggc ctgatcagcc gatagagccg aaggcaggaa
     2401 cccatagccc gactctcgat cggctccctt ggggaccagg agtgaccgcg gtccgctcgg
     2461 agacttcgcg cgttcggcgt tccttggccg gtgtgggacg gtgtggctga attgggcctg
     2521 aagatttcgg gccgccagcg gatcaacggc ggggacggga tccggccggc cgcaataggc
     2581 ccgggccacg gagtggagga accgcagggc gcccacaccg tcggtgacgg catggttggc
     2641 actgagcaga agcgaatcac cgccgggccg atgtgcgagc agcagacgga gcgccgggga
     2701 ccgctccacg tcgatgggtc ggctgtagaa ctgggcccga gccgcggcca gggccgactc
     2761 gtcgccgcaa tcgatggacc gcaggatgtc gcccgccgat ggcccccc
//
LOCUS       DATAQL010000695         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2363310, whole genome shotgun sequence.
ACCESSION   DATAQL010000695 DATAQL010000000
VERSION     DATAQL010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363310"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1124
                     /locus_tag="VHS97_12140"
     CDS             201..1124
                     /locus_tag="VHS97_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3448999.1"
                     /translation="MANLWPGTVSEAQLKTRGEPRAAVADASAGPATALVEPSSPATA
                     RGPVHPAADLEPEVTRRLRAQYAEVSQLAGGLAHEIRNPLSTVSLNLDLLAEDFQHPE
                     TPRDRRVLQRVERLQHEVLRLRDILESFLRFARVQPLRLESINLNAIVEELCDFYEPT
                     AATKGVVIRTYLAPDLPSTAIDADLLKQALLNLILNAEHAMPSGGELILKTHHDGPWV
                     VLDVIDTGVGMTEEVRARIFDAFFSTRPAGSGLGLPTARKIVEAHGGTLTVQSEPGKG
                     SQFTLRLPGSEAGSPRQEPLSWDVSPPTRPS"
     gene            1178..>2546
                     /locus_tag="VHS97_12145"
     CDS             1178..>2546
                     /locus_tag="VHS97_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3449000.1"
                     /translation="MDQQIRVLVVDDDEPHAAAVAESLERVGYDCVVATSGAEGLRLI
                     EEQVFDIVITDLIMEGVGGLEVLAKAKRELPDAEVVILTGHSTIKTAVTAMQAGASTY
                     LTKPLDINELRTKADKASQSQRLQRSNIELQMQLNEKFGFEGVIGNSSAMHSVVSKLR
                     QIAPTSASVLITGESGTGKELAAKALHINSPRKNKPFVPLNCAELSENVLESELFGHV
                     KGAFTGADRDRVGRFQYANGGTLFLDEIGDMPISIQVKLLRVLESGEIVRVGTNEPVK
                     VNVRLISATNRELAEAINDGKFRQDLYHRLKVVSVKLPPLRERREDIPLLIEHFLKEF
                     TASHGKTITAVTPAVRKAIMAYSWPGNVRELRNTIESMVVLDSDGQLDLDDMTEDLQA
                     FTSSAKADGTSGVDSLVGQSLEEIEKHYILETLKRCNGNREEAARQLGIGERTLYRKL
                     KEYNLG"
BASE COUNT          524 a          797 c          767 g          458 t
ORIGIN      
        1 cggcgaccgc gatcgtgatc gcggctacgg cggcgatcgg ggcggcccgc gcggtcccgg
       61 acgtccttga cgccgatgga tttccggccg gacgtttcgt cgtggttaag caatcctggt
      121 agggccctcg cgtagacgga agcggctatt tttagtaatc gctacgcgac cagacttgcg
      181 gcaaccggcg gagttctcgc atggcaaact tgtggcctgg caccgtttcc gaggcacagt
      241 tgaaaacacg gggcgaaccg cgcgccgccg tcgccgatgc atccgccggc cccgcgacag
      301 ccctggtcga gccgtcgtca ccggccacag cgcgcgggcc ggtgcatccg gcggccgatc
      361 tcgaaccgga agtcacccgg cgcctccgcg cgcaatacgc cgaagtttct cagcttgcgg
      421 gcggtctggc ccatgaaata cgaaacccgc tttccacggt ttcgctgaat ctcgatctgc
      481 ttgccgaaga ctttcagcac ccggaaaccc cacgcgaccg tcgcgtgctc cagcgggtcg
      541 agcggttgca gcacgaggtt cttcggctgc gtgatatcct cgagagcttt ctccgcttcg
      601 ctcgtgttca acccttgaga cttgagtcga tcaatttaaa cgcgatcgtg gaggagctct
      661 gcgacttcta cgagccgacc gcggccacga agggcgttgt aatccgcacc tacctggcgc
      721 ccgatttgcc ctcgacggcg atcgacgctg acttattaaa gcaagctctc ttgaacctga
      781 tcttgaacgc cgagcatgcc atgccatcgg ggggcgagct gattttgaag actcaccacg
      841 atggaccctg ggtcgtgctc gatgtgatcg acaccggcgt gggaatgacc gaggaagtac
      901 gagctcgaat ctttgacgcc ttcttctcca cccgaccggc aggaagcggt ctgggtttgc
      961 cgaccgcccg caaaatcgtc gaggctcatg gcgggacatt gaccgtgcag agcgagccgg
     1021 gcaaaggatc gcagtttacc ctacggctcc ccgggagcga agcggggtcc ccgcgccaag
     1081 agccgctctc gtgggatgtt tccccgccca cgcggccttc ttaagagagg cgagtttttt
     1141 ccactcctcg aatcgaacgc gagcgagcaa ttggccgatg gaccagcaga tccgcgtctt
     1201 ggtggtcgac gacgacgaac cgcacgccgc ggccgtggcc gagagcttgg agcgggtcgg
     1261 ctatgactgc gtcgtggcca caagcggcgc agagggcctg agactgatcg aggagcaagt
     1321 gttcgatatt gtgatcaccg acctgatcat ggaaggggtc ggcgggctcg aggtcttggc
     1381 gaaggccaag cgcgaacttc ccgacgccga ggtcgtgatc ctgaccggcc atagcacgat
     1441 caagacggcc gtcacagcca tgcaagccgg cgcctccact taccttacca agccgctgga
     1501 tatcaacgag ttgcgcacca aggccgacaa agcctcgcag tcgcaacgac ttcagcgctc
     1561 caatatcgag ctgcaaatgc agctcaacga gaaattcggc ttcgaagggg tcatcggcaa
     1621 ttcgtcggcc atgcattcgg tcgtctcgaa gctccgccag atcgcgccga cgtccgcttc
     1681 cgttctcatc accggcgaga gcggcaccgg caaggaactg gctgccaagg ccctgcacat
     1741 caacagcccg cgcaagaaca aaccattcgt accactcaac tgcgccgagc tgagcgaaaa
     1801 cgtgctggag agtgagctct tcggccacgt caagggggcc ttcaccggcg ccgaccgcga
     1861 tcgcgtaggc cgctttcaat acgccaatgg gggaacgttg ttcctggacg agatcggcga
     1921 tatgcccatc tcgatccagg tcaagctgtt gcgcgtgctc gagagcggcg agatcgtccg
     1981 cgtgggaacc aacgagccgg tcaaggttaa cgtccgcttg atatcggcga ccaaccggga
     2041 gctggccgaa gccatcaatg atggaaagtt ccgccaggat ctctatcacc gactcaaggt
     2101 ggtcagcgtc aagctccctc cgctgcgcga gcggcgcgaa gatatcccgc tcttgatcga
     2161 gcatttcttg aaggaattca ccgcgtcaca cggcaagacc atcaccgccg tcactcctgc
     2221 cgtgcggaag gcgatcatgg cctattcctg gcccggcaac gtgcgcgagc tgagaaatac
     2281 catcgaaagt atggtcgtgc tggacagcga cggccagctt gacctggacg acatgaccga
     2341 agatctccag gccttcacct caagcgccaa ggccgacgga acttccggtg tcgactcgct
     2401 ggtgggccag tcgctcgagg agatcgaaaa acactatatc ctcgagaccc tgaaacgttg
     2461 caacggcaac cgcgaggaag ccgcacgtca gctcggtatc ggcgagcgga cgctctatcg
     2521 aaaactaaaa gaatataatc tggggt
//
LOCUS       DATAQL010000696         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1904168, whole genome shotgun sequence.
ACCESSION   DATAQL010000696 DATAQL010000000
VERSION     DATAQL010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904168"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1312)
                     /locus_tag="VHS97_12150"
     CDS             complement(299..1312)
                     /locus_tag="VHS97_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX3449001.1"
                     /translation="MWPPPLSETPLEFEVRPRTEGKRLDVYLSNRFSDYSRRVIQKII
                     DADAVLVNGRAAKASYRVRPGDLVSIRLPDLPDTTPKPEDIPIEVVYEDESLTVVNKP
                     AAMVTHPARGNWQGTLVNALQFHFDTLSSLAGENRPGIVHRLDRDTTGLLIVVKDELT
                     HRRLATQFELREVHKEYLAIVYGVPERHSDYIEQPIGFHPIARQKMAIRAPADGGKEA
                     VTFYEVEERFRNFALVRCKPQSGRTHQIRVHLTHIGHPIVADKLYSGRDQLTLGNLKN
                     PDNVTQTDESVTDPTLIDRQALHAHRLRFRHPITERELDLTAPVPADMDRTLDALRAH
                     RAL"
     gene            complement(1328..>2163)
                     /locus_tag="VHS97_12155"
     CDS             complement(1328..>2163)
                     /locus_tag="VHS97_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449002.1"
                     /translation="KFSTWALSLALGVLAVVVVWNLTTGRQIKSSEAWTKLEAAKKAE
                     ERLDVAKEFPNSPASTWAQLQAANEYYNLALADLPNNRDVALPLFKKALDLFDRVASE
                     APKDSFQARAAALGKARSLEARNELAKAIDQYELVAKNWPGTPEADQAKLIAEGLQKP
                     EAAEFYKELYAYSPTKVTLPPYEKEQVDLPPTGINPPGDKMTPPPRPTPAVKIPLDLA
                     PPTVEEKRKLEILEVPAADKPSTPPAKTDSAQNPAAKGELPVDVLSPRANAPTEKAAP
                     R"
BASE COUNT          369 a          593 c          738 g          463 t
ORIGIN      
        1 cgtgctgggc gcaggaaagc cggccgaaga agaccgtctg gccgccacgg tcgcggccaa
       61 ttgagggcaa ggttcaaaag caagttacac ctcagggggt ggctggggtc gacgcccgaa
      121 gggcgacgcc gccagatctg acggtttcgc gccgggggct cgctggctgt cgccagctcg
      181 accccagcca cccagctctt gtcacctgcg aagatgctaa agttgaaccc tcgccaattg
      241 agcgatcgaa agtgatcata ttccgtttcg agcgatcgga ttaaagccga gatcccgatc
      301 acagagcacg atgcgcgcgc agcgcgtcga gggtacgatc catgtccgcc ggaactggag
      361 ccgtcagatc gagctcgcgc tcggtgatgg ggtggcgaaa ccgcagacgg tgggcgtgga
      421 gggcctggcg atcgatcaaa gtcgggtcgg tgactgattc gtccgtctgg gtcacgttat
      481 cggggttttt caggttgccg agcgtcagtt ggtcgcgccc ggagtagagc ttgtcggcca
      541 cgattgggtg gccgatgtgg gtcaagtgga cgcggatctg gtgagttcgg ccgctctgtg
      601 gtttgcagcg gaccagggcg aagttgcgga aacgttcctc gacttcatag aaagtcacgg
      661 cttccttgcc accgtccgcg ggggcgcgga tggccatctt ctggcgtgcg attggatgaa
      721 agccgatcgg ctgctcaatg tagtcgctgt ggcgctcggg gaccccataa acaatcgcca
      781 gatattcctt gtgaacctca cggagctcga actgcgttgc cagccttcga tgggtcaact
      841 cgtccttgac gacgatcagt aacccggtgg tgtcgcggtc gagacgatgg acgattccgg
      901 gccgattttc gccggcaagg ctcgagagag tgtcgaagtg aaactggagt gcgttgacta
      961 acgtcccttg ccagttgcct cgggcgggat gcgtgaccat cgcggcgggc ttgttcacca
     1021 cggtcaggga ttcgtcttca tagacgacct cgatcgggat gtcttcgggc ttcggcgtgg
     1081 tgtcgggcag gtccggaagc cggatgctga ccaggtcgcc gggccggacg cggtaagagg
     1141 cttttgccgc tcgcccgttc acgagaaccg cgtcggcgtc gataattttc tggatcacgc
     1201 gcctggagta gtcgctgaaa cggttcgaca ggtagacgtc gagccttttg ccttcggttc
     1261 gaggcctgac ctcgaactcc agaggtgttt ccgacagcgg cggcggccac atgggtccaa
     1321 tttcgtgtta gcggggagcc gccttttcgg tcggggcatt agcacgtggt gacaagacgt
     1381 caacgggcaa ctcgcccttg gcagccggat tctgggcgga gtcagtcttg gccggtgggg
     1441 tcgaaggttt gtccgccgcc ggcacttcga gtatttcgag cttacgtttt tcttcgacgg
     1501 tcggaggggc caggtcgaga ggtatcttga ccgcgggggt cggccgcggt ggcggtgtca
     1561 tcttgtcgcc tggtggattg atgccggttg gggggaggtc gacctgttct ttctcatacg
     1621 gcggcaatgt gaccttggta ggcgaatacg catagagttc tttgtagaat tccgcggctt
     1681 ccggcttttg cagcccctcg gcgatcagct tggcctggtc ggcttccgga gtgccgggcc
     1741 agttcttggc gaccaattcg tactggtcga tggccttggc aagctcgttg cgggcctcga
     1801 gcgaacgtgc cttgcccaga gcggcggcgc gggcctggaa ggaatctttg ggagcctcgc
     1861 tcgccacccg gtcaaacagg tcgagcgctt tcttgaagag gggaagggcc acatcgcggt
     1921 tgttggggag gtcggcgagc gcgaggttgt aatactcgtt cgctgcctga agttgggccc
     1981 acgtcgaggc tggcgaattg gggaattcct tggcaacatc gagtcgctct tcagcctttt
     2041 tggccgcctc gagcttggtc caggcctcgg aacttttgat ctgtctgccc gtggtcagat
     2101 tccagacgac cacgaccgca agcacgccca gggcaagcga gagagcccac gtcgaaaact
     2161 tcc
//
LOCUS       DATAQL010000697         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5263059, whole genome shotgun sequence.
ACCESSION   DATAQL010000697 DATAQL010000000
VERSION     DATAQL010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5263059"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..410)
                     /locus_tag="VHS97_12160"
                     /pseudo
     CDS             complement(1..410)
                     /locus_tag="VHS97_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011077905.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            720..>1618
                     /locus_tag="VHS97_12165"
     CDS             720..>1618
                     /locus_tag="VHS97_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449003.1"
                     /translation="MAVGGANSGRSVKTTVPARLDRLPWARFHLMVVVALGITWVLDG
                     LEITIVGALSGVLQSEHTLHLSSEQIGTVASCYVAGAVLGAIVFGRLTDRFGRRTMFY
                     VTLLIYLGGVLLTAVSWGMASFAIFRFITGLGIGGEYAAINSAIDELIPARLRGRIDL
                     IVNGSYWIGAGIGAAATILLLNPGLLPVDLGWRLGFGIGGLLGLTILILRRHVPESPR
                     WLVTHGYEEAAEKVVSGIEDAVRKETRQPSLGEPGRALTIHPRKTFGFGLIAKAMLAK
                     YRARSALALSLMVAQAFLYNAVF"
BASE COUNT          278 a          526 c          497 g          317 t
ORIGIN      
        1 gccagcgagg gcgtcgacca gcggcttcaa cgccgccgct atggctccga cccgaccgga
       61 cagggtcgag gctccaggct caccccgtcg cgggcgaaga tctcggcctg gcgatagagc
      121 ggtagatgat cgtcgtactt cgaaacgacg atatgagcca atagtcccgc cccggcgcgc
      181 ccgcgcgcga tcgcatggtc tggcgccggt gccgcaacca ctgtgtcgca aacccggcac
      241 gacagtttct cgcgaatgtg gcggaccact ttgaaccgcc ctgggacgta atcgagggtt
      301 tcggtcgcct catcggcgag cttgcgcaac gcgccgccgc aactggggca ggtgcacggt
      361 gcaggatgcc gcagatcctc tctgggcagg tgttgcggca atgggcgccg cgccgacccc
      421 tggtggaacc cggcggtcga gcaccaggcg accgaccgag cccaccggat cggccagaca
      481 aagcccgtct ttgtccaacc ccagctcatc ccgccaccta cacctgatag gatttgcgat
      541 gaccgtaaac ggccgctctc ggacctgtgg cggccactta gccttcgacg ctgggaacgg
      601 ctcttcctgc cctccccgac cgttagttgg ccgagcacca ctaccgatgc tgcggggaac
      661 ccataatcca ccagaacgta tgtttgcttc tggcacacac tcttcgtgag gatcagtaca
      721 tggccgtcgg aggcgcgaat tctggtcgct cggtcaagac gacggtgccc gcgagattgg
      781 accgactccc ttgggcgcgc ttccacctca tggtcgtggt ggcgcttgga attacttggg
      841 tcctggacgg tctcgagatt accatcgtcg gcgcgctcag cggcgttctg cagagcgaac
      901 acactcttca tctctccagc gagcagatcg gtaccgtcgc atcctgctat gtcgccggcg
      961 ccgtgctggg cgcaatcgtg tttggtcggc tcactgaccg ctttggtcga cgaactatgt
     1021 tctatgtcac cctgttgatc tatctgggcg gcgtccttct cacggcggtc agctggggca
     1081 tggcaagctt cgccatcttt cgtttcatca ctggcctcgg cattggcggc gagtacgcgg
     1141 ccatcaattc ggcgatcgac gagctaatac cagcgcggct cagaggccgg atcgacctta
     1201 tcgtcaatgg gagctactgg atcggggcgg ggataggcgc ggccgcgaca atcctgctcc
     1261 tcaaccccgg cctgttgccg gtggatttgg gctggcggct cggattcggg atcggcggtc
     1321 tactgggcct gaccatcttg atccttcgcc gccacgtgcc ggagagcccg cgctggctcg
     1381 tcacccacgg gtatgaagag gccgcggaga aggtggttag cggcatcgag gacgccgtgc
     1441 gaaaagaaac tcgccagcct tctctgggtg aacccggccg ggcgctcaca attcacccac
     1501 gcaaaacctt cggattcggt ctgatcgcca aggccatgct cgcaaaatac cgcgcgcgat
     1561 ctgcgctcgc cctttcgctg atggtggcgc aagcattcct ttacaacgcg gttttctt
//
LOCUS       DATAQL010000698         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3429594, whole genome shotgun sequence.
ACCESSION   DATAQL010000698 DATAQL010000000
VERSION     DATAQL010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3429594"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VHS97_12170"
     CDS             complement(<1..197)
                     /locus_tag="VHS97_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449004.1"
                     /translation="MSTPVAVEPTQASSSSTGRPKPLLSGPDVLGRFGSFGGRFVPET
                     LMDALNQLAEAYDEAKADTTF"
     gene            complement(399..881)
                     /locus_tag="VHS97_12175"
     CDS             complement(399..881)
                     /locus_tag="VHS97_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449005.1"
                     /translation="MGPAEIVASLKELCAEKGPRLGTDDIVAWIEKIGGFEPYAELVA
                     YAKKMKARQYARMLEYEDETSGMRIKRLWSFRDQSQGRRFYADILELPAEERKQLIRK
                     YARFLKQMRAVRKAMADYFAGQRFFDFYSELAEEEEAEAEIDLSPPPRSRQQSGRATR
                     "
     gene            1187..2596
                     /locus_tag="VHS97_12180"
     CDS             1187..2596
                     /locus_tag="VHS97_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449006.1"
                     /translation="MWVRSAGVRSVLDLVVPAMILVGPFSGAARAGGPADVAPAARSA
                     AAERGRTALTLKGFLKPEWSDAVYRNAARFWDQPAPDPEKDPLEYAAVFRYRYGLHPA
                     PYPNDGLPMGLRRGLGPGGAKTGLQIDCLVCHGGSIGGQSYVGLGNTQLDLKALLFEL
                     TMADGKRPPLSTFVLNTSRGTNNAGQIAAVLISLRNPDLSVRSFPLPLGVNLPEMDTP
                     PWWHLKRKRTMYYDGRTDARSVRTNMQFLLGEKSALDLKALEPAFRDLQAYFLSLEPP
                     KYPFPIDADKAARGEAVFQTTCVRCHGTYDPDGKYPNKIVPLDEIGTDPARSQGLSDR
                     LVAHYNKTWLGAEYPVDTKRVGYQAPPLDGIWATAPYLHNGSVPTLHALLDSLSRPPR
                     FTRPPSTDFAHYDQAHLGWKFTEVSADDLSSTARRSPFQAKFIVDTARFGMSNGGHTF
                     GDALSEEERMDLIEYLKTL"
     gene            complement(2601..>3498)
                     /locus_tag="VHS97_12185"
     CDS             complement(2601..>3498)
                     /locus_tag="VHS97_12185"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX3449007.1"
                     /translation="PDTLEMAYTADDVERIAKNGKIASLIGIEGGVAIENSLAQLRAF
                     HALGARYMTLTHNSTLDWADAANDAAKHDGLTPFGERVVKEMNRLGMLVDISHVSPAT
                     MADALRVSQAPLIASHSSAFAICPSPRNVPDEILKEVTKNGGVVMVNFYSGFVVPESG
                     KMMRKVMDEMRTKYPDRAARTKAMEEWFKTDAAKMSRGTYRDVADHIDHLVKVAGIDH
                     VGIGSDFDGITMWPVGLDDVSSYPRLTDELLRRGYSETDIHKILGANALRTFRQAGEV
                     AKRLRATTAPEVDEIKPERRGG"
BASE COUNT          639 a         1075 c         1052 g          732 t
ORIGIN      
        1 tggaacgtag tgtcggcctt ggcctcgtca taggcctcgg cgagctgatt gagcgcatcc
       61 atcaaggttt cgggcacgaa ccgaccgccg aacgagccaa accggcccag gacgtctggg
      121 cccgagagca atggcttggg gcgaccggtc gacgacgagc tcgcttgcgt cggttcgacg
      181 gcgacaggcg tagacatggg caggcgatcc ttggtgggat tggccggggt ttgattttcg
      241 gtacgcgcgg cgtccgcgaa tgcagatgaa cgacgcagtc agtgtattct cccggctcgc
      301 aaacggcacg gtcaagggta aggactgcgc cggccggggc agacttgctc ccttcggctc
      361 gaatttgctg ccggtttcaa ggcccgttct cgagtcgcct atctcgtcgc gcgtcccgat
      421 tgctgacggg accgaggcgg tgggctcaag tcgatctcag cctcggcctc ttcctcttct
      481 gccagctcgg aataaaaatc gaaaaaccgc tggccggcga aatagtccgc cattgccttg
      541 cggacggccc gcatttgctt gaggaaacgc gcgtacttgc ggatcaactg cttgcgctcc
      601 tctgcgggca actcgaggat gtcggcatag aaccgacgtc cctgggactg atcccgaaaa
      661 ctccagagcc gtttgatccg catcccggag gtttcgtcct catattcgag catccgggca
      721 tactggcggg ctttcatctt cttggcgtag gcgaccagct cggcgtaggg ctcgaagccg
      781 ccgatctttt cgatccaggc gacaatgtca tcggtgccca accgcggccc tttttcggcg
      841 caaagctcct tgaggctggc aacaatctcg gcgggtccca tggtgcgctc cctcaatggg
      901 tccggtcact gtcgagcggt atcctaaaat ccgctcgaat cggaaaacat actccattta
      961 gcgggattct tgccgctgtg tccagatgtg ttaatggact ctcgtacaat taccgcggtt
     1021 tgtgaggggg ggcctcccgt ggtcgcgccc ggttaaggtt ggtcgagtga tcgcggtttt
     1081 agctgacgag agaatgccgg ttattcccgt tcgcccctgg ggcgcttata acagaggaat
     1141 accgtggaga atccgcgaag gttttcagaa cggccgaggt ggctccatgt gggttagatc
     1201 ggcgggcgtg cggtcggtgc tggacctggt tgtgccagct atgattttgg tcgggccgtt
     1261 ctccggtgcg gcccgggctg gtgggcccgc ggatgtggca ccagccgccc gaagcgccgc
     1321 ggccgagcgc gggcggacgg cactgacgtt gaagggcttt ttgaagcccg aatggtccga
     1381 cgccgtctat cgcaacgcag cccgtttttg ggatcagccg gctcccgatc ccgagaaaga
     1441 cccgctagaa tacgctgcgg tttttcgcta tcgctacggt ttgcatccgg cgccctatcc
     1501 caatgacggc ctgccgatgg gcctgcgccg ggggctcgga cctggcggtg cgaaaaccgg
     1561 cctgcagatc gattgcctgg tctgccatgg tggatccatc ggcgggcaga gctatgtcgg
     1621 tctgggcaat acgcagctcg acttgaaggc gttattgttc gagctgacca tggccgatgg
     1681 caagcgcccg ccgctttcca cattcgtgct caacacgtcg cgcgggacca acaacgccgg
     1741 ccagatcgcc gctgtcttga tcagtctgcg caatcccgac ctgtcggtcc gttcctttcc
     1801 gttgccgctg ggggtaaatc tcccggaaat ggacacaccc ccttggtggc acctgaagcg
     1861 caagcggacg atgtactacg acgggcggac agacgctcgg tcggtccgga cgaatatgca
     1921 gttcctcctc ggtgaaaaga gtgctcttga tttgaaggca ctcgagccgg cttttcgcga
     1981 cctccaggca tacttcttga gtctcgaacc ccccaagtac ccgttcccga ttgacgccga
     2041 caaggccgcc cgcggcgaag ctgtattcca aacgacgtgc gtccgctgcc atggcacgta
     2101 cgatccggat gggaagtatc ccaacaagat cgtccccctg gacgagatcg gcaccgatcc
     2161 ggcgcgttcg cagggcctgt cggatcggct ggtcgcccat tacaacaaaa catggctagg
     2221 ggccgaatac ccggtcgata ctaaacgtgt cggctaccag gctccgccgc tcgacggcat
     2281 ctgggcgacg gccccctacc tgcacaatgg gtccgtgccg accttgcacg cgctcctcga
     2341 ctcgttaagc cgtcccccgc gttttacccg tcctccctcg accgacttcg cccactacga
     2401 ccaggcccac ctcggctgga aatttaccga ggtaagcgcc gacgacctct catcaaccgc
     2461 ccggcgatcg ccgtttcaag ccaagttcat cgtggatacc gcacgcttcg gcatgagcaa
     2521 cggcggccac acttttggcg acgctctgtc cgaagaagaa aggatggacc tgatcgaata
     2581 tctcaaaact ctctgaactt tcagccgccg cgccgttccg gcttgatctc atcgacttcc
     2641 ggagcggtgg tcgctcgcag ccgcttggcg acttcgcctg cttggcgaaa agttcgaagg
     2701 gcgttggccc ccagaatctt gtgaatgtcc gtttcgctgt atcctcttcg taagagctcg
     2761 tcggttaacc gcgggtaact cgagacgtcg tccaagccga cgggccacat ggtgatcccg
     2821 tcgaagtccg agccaatacc gacgtgatcg atgccagcga ccttgaccag atggtcgatg
     2881 tggtcggcaa catcgcgata cgttccccgg ctcatcttgg cggcatctgt cttgaaccac
     2941 tcttccatgg ccttggttcg agccgctcgg tccgggtatt tggtccgcat ctcgtccata
     3001 accttgcgca tcatcttgcc ggactcggga acgacaaagc ccgagtagaa gttgaccatg
     3061 acgacaccgc cgtttttcgt cacttccttc aagatctcgt cgggaacgtt acggggagat
     3121 ggacagatgg caaacgcact cgagtggctg gcgatcaacg gcgcctgaga gacgcgcagg
     3181 gcgtcggcca tggtggccgg cgaaacatgc gagatgtcca ccaacatgcc cagccggttc
     3241 atttccttga cgacacgttc accaaacggg gtcaggccgt cgtgcttggc ggcgtcattg
     3301 gcggcatccg cccagtcgag cgtcgagttg tgtgtcaagg tcatgtatcg agcgccaagc
     3361 gcgtgaaagg cacgaagctg ggccaggcta ttctcgatcg ccacgccccc ttcgatgccg
     3421 atcaacgacg cgatcttgcc gtttttcgca attcgttcga cgtcatcggc cgtgtaagcc
     3481 atttcgaggg tgtcggga
//
LOCUS       DATAQL010000699         5976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3890500, whole genome shotgun sequence.
ACCESSION   DATAQL010000699 DATAQL010000000
VERSION     DATAQL010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890500"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2315)
                     /locus_tag="VHS97_12190"
     CDS             complement(<1..2315)
                     /locus_tag="VHS97_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HEX3449008.1"
                     /translation="MTARRHDVPVFQPAGEPLEAKVLMTIDLGGTSPPALPFSATAPF
                     GVDVGAATPNGGAGWSVADVGDLTGSGYDDVLIGAPTITTPSTLGTGVGKVYLVLGSA
                     TVNASNPAAVQNWLNTVTGPNLAPNDRVGDLAQLGATAQTNPISGTALNFPFSGVTFT
                     GVASLGASVAAVRISTTQNGILIGAPNSASGVGTAYFISGNFALYLGKTVDLSNPVAS
                     PGLNIVTFRNTATFGAGGALGTSVAGGFNILGDGVGDIILGAPGASVASTNPTTPVTQ
                     NTGVVYVISTGFLSGSTQTIDVSTLNTSRAVTFAGAATGDKAGFSVADAGDVNGAAGS
                     VDDLLIGAPASASEAGSAYLVYGGSTLAGLQTTVNNVPFISLSRLSGGTGTGTPIPGA
                     TFVGPAGAVPGSQLGWTVSAGGDFNGDGNADILLGAPFWSGSTTSTQAGLVYILYGAP
                     STSSAFLTGRISLANIPTAIQSAVIVGAAANNLAGYSTSQVGLINTGQPTSILIGAPG
                     FNANSGTAYLIPGRAGFTGGPFSLATAESAPLSGIQFFNTTPTSQSTSPPFFGASVSS
                     RIQGTQLFTVDRDKEADFIIGAPGYDVTQNASRTLAGGAQIVQSGFLTVPIPTANTIT
                     TTIGVGTPFAPFAINATTPTTLQIFVFGSTTTTPAFMPVTDIDPTSVVVNGVPFPNAT
                     LTPDADAKDHVPGGIVDAIITISPRASLNLPNGNVNITISGKTLATSPLSNFTWRGTA
                     SVAVTGGSGGGGGGPTVSAVVGPPTGPNLQIV"
     gene            2900..>5976
                     /locus_tag="VHS97_12195"
     CDS             2900..>5976
                     /locus_tag="VHS97_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449009.1"
                     /translation="MRKSRNSRFRVTTRQLGIGAILTALALGLQSGCTREFYREWANQ
                     DVSETVFEKSRDPRWRMDLFSVEPPALSRFADPYDQEVPPAPPDDPAAEALSPAPQWP
                     DDRLLVPLEGTGYLDLLEYWRRDEQAKKRAAGEKVPEDEPEFWQRPDSGRHAITDPRQ
                     PGMPGQPLSSPTGPPVPPETSSPFSNPPSPPPSPRGGVSAPPGPQASTTGTQARLGSN
                     PSPPVIRLQDGGQSQLATRGSSLTTGAKQPAAANSGTNAQDSTPISISRVTATAVKPR
                     IPPPRSGASFTNAPTSGNKAAKDKALVRASIKDNGTPAASAADAGTGMRTEILQMAAP
                     SQDQPAPGPPGPLQGQPPQATPPQDRPPVASPSNILDREINAQPPMLDRSQLDAAGRM
                     TQTEAAELAGVLVPRVDLQNDTEGYGFPKGSRVYKINMQQAWLLALMNARFYQFNLEQ
                     VYLAAIPVTLQRFIFGPQFFAGLSPVTSPLTPGVLGGASAGFPSTPGVATNSTFTYAT
                     RFSPAGQVSTLNLGTVAGFGKLFSTGGELLMGFANQVLFNFAGKNPKQPTVISALPIS
                     FVQPLLRGGGRAVTLEPLTQVERNLLYAVRAFALFRQQFFVVTLTGGTIQNFGQTFNL
                     AGFSSAGNSDPTIGFLPIVFNLVEVEVDRRNLFFLESVARLYNELIQGEASGLSRLQL
                     DQVMQRVIAGRQALFVDKVTYRVQLDEFKMQMGLPPDTPVVIDMSLAQPFYDVFNAVD
                     EWQRRSTRKLDELPFIIAKLPELEDIDVEGRSVLAPYRNYRSTLKEFAPENEEGLEDT
                     LQAAVRIALEYRMDLMNDRAQLYDAWRQIRVTANALKGVLNIAITNNIYTAPNPSNPF
                     EFLSQAKQFGLTLNSELPLVRINERNAFRTALITYQRTRRALQTAEDNLKIQLRNDLR
                     QVHLGYINYEIAKRNYELNVRLKDQAFEQIIAPPAAGGGGLAQTVNAATQTTNLLNFQ
                     NQLIMATLALTNGWQFYQTQRLIVYRDIGILPYDEWEAFSELYPAQYHGPIIG"
BASE COUNT         1188 a         1834 c         1844 g         1110 t
ORIGIN      
        1 acaatttgca aattcggccc ggttgggggt cctacgacgg cgctgacagt cggaccgcca
       61 ccgccgccgc cactgccgcc ggtgacggcg accgacgctg tgcctctcca cgtgaaattg
      121 gacaacgggg atgtcgcaag cgtcttgccg ctaatcgtga tgttcacatt gccgtttggc
      181 aggttgaggc tggcgcgggg gctgatggtg ataatggcgt ccacgatgcc gccggggaca
      241 tggtctttgg catcggcgtc tggcgtcagg gtcgcgttcg ggaagggtac tccattgacg
      301 acgacggacg tgggatcgat gtcggtgacc ggcatgaaag cgggggttgt ggtcgtcgat
      361 ccgaagacaa agatctgaag ggtggtcggc gtggtcgcat tgatggcgaa gggggcaaac
      421 ggggtgccga cgccaatcgt ggtcgtgatg gtattggccg tcggaattgg gaccgtaagg
      481 aatccgctct gcacgatctg agcgccgccg gccagagtgc gcgatgcatt ttgggtgacg
      541 tcgtatcccg gcgctccgat gatgaagtcg gcctctttgt ctctatcgac cgtgaagagc
      601 tgagtgcctt gtatacgact cgataccgag gcgccgaaga agggcgggga ggtcgattgc
      661 gaggtcggcg tggtattgaa gaactggata ccggaaagcg gggccgattc agccgttgcg
      721 agcgagaagg gccctcccgt aaagcccgca cggcccggaa tcaggtaggc agttccggag
      781 ttggcgttga agccaggtgc gccaatcaga atcgaggtgg gttggccggt gttgatcaac
      841 ccgacttgcg acgtcgaata gcccgcgaga ttgttggcgg cggctccgac gatcactgcc
      901 gactggatag cggtcggtat gttggccagt gagattctgc ccgtcaagaa agcagaggag
      961 gtgctgggcg ctccataaag tatgtagaca aggcctgcct gggttgaggt tgtgctaccg
     1021 ctccagaagg gggcgccgag cagaatgtcc gcattcccat ccccgttgaa gtcgccgccc
     1081 gccgagacgg tccagccgag ttggctgcca ggtaccgcac cggcaggacc gacgaaagtg
     1141 gcccctggta tcggtgtgcc tgtcccggtt ccgcccgaaa gtctcgacag gctgatgaag
     1201 ggtacattgt tgaccgtcgt ttgcaagccg gcgagtgttg agccaccgta gacaagatag
     1261 gccgatccgg cctcggaagc cgaggcgggg gcaccgatca agagatcgtc gacgcttccg
     1321 gcggcaccgt tgacgtcgcc ggcatcggca acggaaaagc ccgctttgtc gccggttgcc
     1381 gcgccggcaa aggtcaccgc cctcgaggta ttcagggttg agacgtcgat cgtttgagta
     1441 ctcccgctca ggaaccccgt cgaaatcacg tacaccacgc ccgtattttg ggtaacgggg
     1501 gtggtggggt tggtcgaggc gaccgaagcg cctggcgccc ccaagatgat gtcgccgaca
     1561 ccatcgccca agatattgaa cccgccggca acagaggtac cgagcgctcc gccggcaccg
     1621 aaggtcgcgg tgttccggaa cgtgacaatg ttcaggccgg gagaagccac cggattggaa
     1681 agatccactg tcttgccgag atatagcgcg aaattgccgg aaatgaagta cgccgtgccg
     1741 acgccgctgg ctgagttcgg tgccccgatc aatatgccgt tttgggtggt cgagatcctg
     1801 acggccgcga ctgatgcgcc cagcgacgcc acgccggtaa atgtaactcc cgagaatgga
     1861 aaattgagcg cggtgccgct gatcggattg gtctgggccg tcgcgccgag ctgggcaagg
     1921 tcgccgacac gatcgtttgg cgccaggttg gggccggtaa ccgtgttgag ccagttctgc
     1981 acggcggccg ggttcgacgc gttaaccgtc gcggatccaa ggaccaggta aaccttgccg
     2041 acaccggtgc ccaaggtcga cggtgtggtt atcgtaggcg ccccgatcag cacgtcatca
     2101 taacccgagc cggtgagatc gccgacgtcg gcgacgctcc agccggctcc gccgttgggg
     2161 gttgcggcgc caacgtccac gccaaacgga gccgtggcgc tgaacgggag agccggtggc
     2221 gacgtgcccc ccaggtcgat ggtcatcaga accttggctt ccagcggctc gcccgcgggc
     2281 tggaaaaccg gaacgtcgtg ccgccgcgcg gtcattttcc gaggtcggcg attgccaaaa
     2341 accattggag actccctcct cccaaaccca taggtcgcgc tcgccgcgtc cggtttcacg
     2401 tccgcggtac gccatcccgg aatatccagc tcgagcggcc acctccatgg gtgcctctcg
     2461 ctctggacca gcgcaattgt gcgtggcaca acctttccgg ccgttcgatg gcggacgccg
     2521 gctctctgcc cgcgccccct ggccgccaag ggtgtcctgt tacatctgga atttcggtcc
     2581 gaaatggctc ttgactccac ccaaatcaga acttgaagat cccgcacgat ccattcaccc
     2641 cttgctttgg gctcaactca tagaactcct atgcccgata tatttcgctg cacggacctc
     2701 ccagccggcg ctgctcttac atagcgcacg ctgcttgaac gagagagcag ccaaacatcc
     2761 acgcggccga ccgccggacg accgttcgat gatctcggga ggctggtcag tcgggatggc
     2821 aataccaaca cgaccatccg ggagcccgtc agtcagaaat ggcggccctg ggcgcgacta
     2881 taagttgtcg aggcgagata tgagaaaatc gaggaattcg agattccggg tcaccacgcg
     2941 acagctcggg atcggagcga tcctcaccgc tttggcgctc gggctgcaat ccggctgtac
     3001 ccgagagttc taccgcgagt gggccaacca agatgtatcc gaaacggtct tcgagaagag
     3061 cagagatccg cggtggcgga tggacctctt ctcggtggaa ccacccgcgc tctcccggtt
     3121 tgccgatccc tacgaccagg aagtgccgcc ggcgccgccc gacgatccgg ccgccgaggc
     3181 cctttcaccc gctccgcagt ggcccgacga ccgcctattg gtgccgctcg agggaaccgg
     3241 ttacctcgac ctcctcgagt actggagacg agatgagcaa gccaagaagc gtgccgcggg
     3301 cgagaaagtc cccgaggatg agccggagtt ttggcagaga ccggatagcg gccggcacgc
     3361 gatcactgat ccccgccagc cgggaatgcc cggccagccg ctgtcgtcgc cgaccggtcc
     3421 gcctgtgccg cctgagacaa gctcgccgtt ttccaatccg ccgagtccac ctccctcgcc
     3481 gcgcggcggc gtgtcggctc cgcccggtcc ccaggcatcg accactggga cgcaggcgcg
     3541 cctgggtagc aatccgtcgc caccggtcat ccggttgcaa gatggcggcc agagtcaact
     3601 agcgacgcgc ggaagttcgc tgacgaccgg ggcgaaacag ccggcggcag cgaattctgg
     3661 gacaaacgca caggattcga cgcccatttc aatatccaga gttaccgcta cggccgtaaa
     3721 gccgaggatc cccccgccgc ggtccggggc gagtttcaca aacgccccaa ccagcgggaa
     3781 taaagccgcg aaggataaag cgcttgtgag ggcatcgatc aaggataacg ggacgccggc
     3841 tgcgtcggcg gcagatgctg gaacaggcat gcggaccgag attcttcaaa tggcggctcc
     3901 ctcacaagac caaccggcgc cgggaccacc tgggcccttg caggggcagc cccctcaagc
     3961 gacccctccg caagaccggc ccccagtggc gtcgcccagc aacatcctcg accgcgagat
     4021 caacgctcaa ccgcccatgc tcgatcgcag ccagctagat gcagccgggc gaatgactca
     4081 gaccgaggcc gcggaattgg ccggcgtctt ggttccgaga gttgatctgc aaaacgacac
     4141 tgagggctac ggatttccca aagggagccg cgtctacaag attaatatgc agcaggcctg
     4201 gttgctggcg ctcatgaacg ctcgattcta tcaatttaat ctcgagcagg tctacctggc
     4261 cgcgattccg gtcacgctgc agcgattcat cttcggtccg cagttctttg ccggactgag
     4321 cccggtgacg agtccgctga cgcccggagt actgggaggg gcctcggccg gcttcccctc
     4381 gactcccggc gtggctacga acagtacgtt cacgtacgca acgcggtttt cgccagccgg
     4441 tcaggtctcg acgttaaacc tgggcacggt cgcggggttc ggcaagctgt tcagcaccgg
     4501 cggtgagctc ttgatgggtt tcgccaacca ggttttgttc aatttcgcgg gcaaaaaccc
     4561 caagcagccc accgtgattt ccgcgctacc gatcagcttt gtccagccgc tcttgcgggg
     4621 cggtggccgg gccgtcacgc tcgagcccct cacgcaggtc gagcgcaact tgctctatgc
     4681 cgttcgtgcc tttgccctgt tccgccagca gtttttcgtt gtgacactga ccggcggcac
     4741 gatccagaac ttcggccaaa cgttcaatct ggccggattt tcatccgcgg gcaacagcga
     4801 cccgaccatc ggctttctgc caatcgtctt taacctcgtg gaggtcgagg tcgaccggcg
     4861 caatctgttc tttctggaaa gtgttgctag actctataat gaattgattc aaggtgaggc
     4921 gtcgggcttg tcgcggttgc agctcgacca ggttatgcag cgcgtcatcg ccggacgcca
     4981 ggctctgttc gtggacaagg tgacgtaccg ggttcagctc gacgaattca agatgcagat
     5041 gggcttgccg cccgacaccc cggtcgtgat cgacatgagc ttggcccaac ccttttacga
     5101 cgtctttaac gctgtcgacg aatggcagcg ccgctccacc cgtaagttgg atgaactccc
     5161 gtttatcatc gccaagctcc ctgagctcga ggatatcgac gttgaaggcc gatcggtgtt
     5221 ggccccctac cgcaactatc ggagcacgct caaagagttt gcccccgaga atgaagaagg
     5281 cctggaagac acgctgcagg ccgccgtgcg aatcgcgctg gagtaccgca tggatctgat
     5341 gaacgatcgt gctcagctct atgacgcctg gcggcaaatt cgtgtcacgg cgaacgccct
     5401 caaaggggtg ctgaacatcg caatcaccaa caatatctac acggcgccga acccgtcgaa
     5461 cccgtttgag ttcctcagcc aggccaagca gtttggcctg acgctcaact ccgagctacc
     5521 cctggtgaga atcaacgagc gcaatgcttt ccgtacagcc ttgattacgt accaacgtac
     5581 ccggagggcg ctccaaacgg ccgaagacaa cttgaagatt cagcttcgca atgacttgcg
     5641 tcaggttcat cttgggtata tcaattacga gatcgccaag cgaaactacg agttaaacgt
     5701 acggctcaag gaccaggcat tcgagcagat catcgctccg ccggccgccg ggggcggagg
     5761 actggcgcag actgtcaacg ccgcgaccca gacgactaac ctgctcaact tccagaatca
     5821 gttgatcatg gcgacgctcg ccctgaccaa cggctggcaa ttttatcaga ctcaacgatt
     5881 gattgtttac cgcgacatcg gcatcttgcc atatgacgaa tgggaggcgt tcagtgaact
     5941 ttaccctgca caataccacg gccccatcat cggcca
//
LOCUS       DATAQL010000700         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4195073, whole genome shotgun sequence.
ACCESSION   DATAQL010000700 DATAQL010000000
VERSION     DATAQL010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4195073"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VHS97_12200"
     CDS             <1..791
                     /locus_tag="VHS97_12200"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HEX3449010.1"
                     /translation="LELGPRTEREIQESLKKEVEAERWTSFDRALRNIADDSAGFVDL
                     RLGASEKDQELRGLLIGRASRLERLGLAEPIGPAQWSLKPDIESTLRDLSARGDIIKT
                     MHQAIRCAGREPDVSGFALHGDEPVAPVVGRLVERGLQDELKGTAYAIVEGVDGRTHH
                     LRFADLELTGDAELDAIVEARSYSDANGRKRLSLATRSDLAIEAQVSASGATWLDRQL
                     IARGPRTGRRRLRSAWRGAHGPGRRTSDRTRSPRRVEGNGLCDR"
     gene            1011..1415
                     /locus_tag="VHS97_12205"
     CDS             1011..1415
                     /locus_tag="VHS97_12205"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HEX3449011.1"
                     /translation="MGSGFGAEVLQAMDRRVDHLIEQGLAARQGQRIVFAGGLLNTLR
                     QTEIDSAAARLSAETGLVHHPSAEREHVAGVYRRRVTLASGRFAMLDDGVGFQLVPWR
                     PALDRNLGQHVSGVITASGIDWDFARKRGLGL"
BASE COUNT          293 a          521 c          541 g          283 t
ORIGIN      
        1 cgcttgagct cggcccccga accgagaggg agatccagga gagcctcaag aaggaggtcg
       61 aggccgaacg ctggaccagc ttcgaccgag cccttcgaaa catcgctgac gacagcgccg
      121 gctttgtcga tcttcgccta ggcgcgagcg agaaggatca ggaactccgc ggcctgctca
      181 tcggccgggc gagcaggctc gaacgccttg gcctggctga gccgatcggg ccggcgcaat
      241 ggagcctcaa acctgacatc gaatcgaccc tgcgcgatct gagcgcccgc ggcgacatca
      301 tcaagaccat gcaccaggct attcgctgcg ctggtcgcga gcccgatgtc agcggcttcg
      361 ctctgcacgg cgacgagcct gtggccccgg ttgtcggacg gctcgtcgaa cgcggtcttc
      421 aagacgagct gaaagggaca gcctatgcga tcgttgaagg ggtcgacggg cggacccatc
      481 atctccggtt cgccgacctt gagctgaccg gcgatgcgga gctcgacgcg atcgtggaag
      541 cccgctcata ttcggacgcc aacggaagaa agcggctatc actcgcgact cggtcggatc
      601 tcgccatcga ggcgcaagtc tccgcctctg gcgcgacctg gctcgatcgg caacttatcg
      661 cgcgcgggcc gcgaaccgga cgtcggcggc ttcgctctgc atggcgagga gcccacggcc
      721 ccggtcgtcg gacgtctgat cgaacgcggt ctccgagacg agttgaaggg aacggcctat
      781 gcgatcgttg aagggatcga cgggcggacc catcatctcc ggttcgccga ccttgagctg
      841 accggcgatg cggagcgcga cgcgatcgtg gaagcccgct catattcgga cgccaacgga
      901 agaaagcggc tgtcactggc gactcggtcg gatctcgcca tcgaggcgca agtctccgcc
      961 tccggcgcga cctggctaga ccggcaactt atcgctcgcg ggcgctgccg atgggcagcg
     1021 gctttggagc cgaggtgctg caggccatgg accgacgcgt cgaccacctg atcgaacagg
     1081 gtcttgccgc gcgacagggc caaaggatcg tgttcgccgg cggcctcctc aacacgttgc
     1141 ggcagactga gatcgacagc gcggctgcca ggctatcggc cgagacaggt ctggtgcatc
     1201 atccgtccgc cgaacgcgag catgtcgcag gggtctatcg ccgccgcgtc accctcgctt
     1261 ccggtaggtt cgcgatgctc gacgacggcg tgggattcca gctcgttccc tggcgaccgg
     1321 cgctcgatcg caacctcggc cagcacgtat cgggtgtgat cacggcaagt ggcatagact
     1381 gggatttcgc tcgaaagcgt ggccttggcc tttaggctgg gcgccgacat tcttttggga
     1441 gactaatttc gctcgcggtg gtgcagctgc ggcgcgcttt cccaaagcac acgagtccga
     1501 gtgccgggtt gaaagacagc cttgccgcga agctttataa ttctccacta cgttgcccat
     1561 gatggggtgc ttcccgaccc aactcggacg ttgagctgga accatcgacg agtccgctcg
     1621 tggccggtag cggtcggg
//
LOCUS       DATAQL010000701         9743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_833884, whole genome shotgun sequence.
ACCESSION   DATAQL010000701 DATAQL010000000
VERSION     DATAQL010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833884"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..753)
                     /locus_tag="VHS97_12210"
     CDS             complement(43..753)
                     /locus_tag="VHS97_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX3449012.1"
                     /translation="MSTRVRWLGHAALLLESDGRNVLIDPFLTGNPKAVVSAELVPAD
                     LILISHGHGDHVGDAVAIAQRTGATVATNYEISEWLQKPAQGLSGPKVHGLQHGGSFV
                     FDQSVRVKLTLAFHGSALPDGSNGGNPCGFVLTFPDGTKIYDAADTALFGDMKLIGEE
                     GLDLAILPIGDYFTMGPDDALRSVKMLQPRFVLPIHYNTFPLIAQDAAAWATRVTNET
                     AAQPVVLQPGDWFDIPRK"
     gene            1026..1985
                     /locus_tag="VHS97_12215"
     CDS             1026..1985
                     /locus_tag="VHS97_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEX3449013.1"
                     /translation="MSQPIDVDSPRKDHVVGGQEHTAPVGEPGSGGCSQCQALQARIA
                     ELEARLEGRSEVGSEAMSSPGPALSCLEFKALRLLPWGWGEGWQLRPSPARRHWMDLS
                     PHAYKCLPMVIANQWGWQILCPTEVTVTWDGTPNPSGVSVEVAPQYRPAIKSQFGSGI
                     VTFSPPWLFRTPSGWDLYLKGPSNRWKDNCVPLEGVIETWWLNYTFTLNWKLVQPGTV
                     TFAEGESLGQLVPVPHATFLDATALEAPIGLIEPRAAEELLEWQEKRRQLAQQAVTVH
                     HLYRKAEGIDEHLQRVPVPPVKPIEPWNHAPREASPDGPTPAG"
     gene            complement(2108..3340)
                     /gene="glgC"
                     /locus_tag="VHS97_12220"
     CDS             complement(2108..3340)
                     /gene="glgC"
                     /locus_tag="VHS97_12220"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX3449014.1"
                     /translation="MYRDVLAIVLAGGRGTRLEPLTRDRAKPAVPFGGIYRIIDFTLS
                     NCINSDIRKILVLTQYKATSLNRHLDQGWRFLSRELNEYIEVIPPQQRIAEMWYQGTA
                     DAIYQNVYTIEKAAPRDTLILAGDHIYKMNYARMIDYHRDNRADLTIACQPVPRHQSR
                     EFGVIGVEGDHRVMAFLEKPKDPQVMPDQPDMVLASMGIYVFNTDVMYELLFQDAARK
                     EASNHDFGRDIIPAMIAGGSRVFAYPFRDENRKEAAYWRDVGTLDAYFQTTMDLIQID
                     PILNLYDQDWPIHTHQPPYPPPKFVHTEGDRRGAAYNSIVCQGAIISGGQVYRSIVSP
                     GVRVNSFALIEDAILFDGVHVGRNARIRRAIIDKDVRIPMGFEIGWNRDADLARGFTV
                     TEDGIIVVAKGEELERFG"
     gene            3826..5532
                     /locus_tag="VHS97_12225"
     CDS             3826..5532
                     /locus_tag="VHS97_12225"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX3449015.1"
                     /translation="MYVILIGLPGRAAGENHSDRLKELQTSYVVSASQKMPRAYHFGS
                     QEPADVFSNHASHTNRLVPVYVFGGKSALSAVTGANSKYRDPEKIKALYGFLPQHTVN
                     PQADYADQSDLYWVQKEAISRGVKHLFIVWFDGLDWPTTQAAAIVKTGRVYTEGKGSG
                     LIFQDYDAGGRAQYGFVVTSPTHEQNVPNLDAQTVVIPPDSLGGGYDARIAGSEPWSL
                     GPLGLKARGYLKGQSASDADRAGVLAVGGVLHAYTDSSTSAAEMVSGVKSYNNGLNVT
                     DDGRLVTTLFHQLQDQGWKVGTVTSVPFNHASPAAMYTQDVYRDDYQDLAREMLGLPG
                     ILQQARRVLLRPGLDVVMGTGFGVITTPKSLAAQGTNGVLGSLFITDADLAAVDVKNG
                     GKYIVSHTEPGASGGQLLKRAATEAARRSARLFGFFGRPGLDHLPYQTADGHYDPAPS
                     LNSSGKLSPAESYSPADLTEQPTLAQMTEAALTALGGQPSQPFALFIEAGDVDFALHA
                     NNLDNAIGAVYSGEEAIRTVIRWVETHSNWDDSLMIVSSDHGHYLVVDDPQALAAAKQ
                     AK"
     gene            5574..6857
                     /locus_tag="VHS97_12230"
     CDS             5574..6857
                     /locus_tag="VHS97_12230"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3449016.1"
                     /translation="MITQHCRTRPLVAPLAFAIALFCGSAGSGSSRQSSEPPATGAVR
                     HCPQLLQPGDPHTHGLSDADLESLSAVLRTAVDNHTVPGVTLLLAHKGEVIFKKSFGN
                     LAVDHKALIASSSKPVTATILMILVDQGKLSLDDPVEKYLPEFKGIAINRRPPAKLPT
                     VRHLLCNMSGLPGDFLAESLLKRLRNGAGNAQDEIKGKELEKVNSSGRFAAGGNRSLA
                     DSVRELVKGGLATEPGAEFHYCTMGFNVAARVAEVAVKRPFEELSRTELLEPMGMKNT
                     RYTPFGLQALSSRPTLSNGESRFIMAGGGMTSTLDDFAAFYQMHLNGGTYRDRRILSE
                     QAVATMHTRQAKLELLMSGPYGNDYGLAFFLDRLDEKGEARVITHPGLFGTTPWFDKD
                     RDLVGILFVQSNFLRVTSLVHEVQAKLRAICKRSP"
     gene            7226..7633
                     /locus_tag="VHS97_12235"
     CDS             7226..7633
                     /locus_tag="VHS97_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449017.1"
                     /translation="MQHWRGQFWLFVFLAVVWPGCGLSDGAPPVSSSLEEATVKGTVR
                     VRSKTVNNGQIAFHSANVSRPNAAIREVPIAKDGTYSITTLVGANTVEVDCNELRDPK
                     TRRRLQHNLAFDVQAGENTFDIDITGMAPAPTR"
     gene            7762..8895
                     /locus_tag="VHS97_12240"
     CDS             7762..8895
                     /locus_tag="VHS97_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449018.1"
                     /translation="MKRIGPRSGFTLIELLVVIAIIAVLIALVLPAVQAAPEAARRLL
                     CVNNLKQISLASHSYANTYGSFPIGHGPVNYNDWGGTAFILPFAEQTPLYNSINFVYG
                     GANPVGVKLPNPVLGVGSRVNINSTVFGTYINMYNCPSDGRDAYTYSSGVGYNPPHFN
                     YTASAGAIPLDDWLFYGGTSQCDGVYCHVDGSSVQPYATALGAPTGFVVSVAMITDGL
                     SNTAAWSERVRGIGFAENGLVDSQPPSTTLWYIPPFTQGGSLADVPIVYNNCIASTTV
                     YSSAVSPSGSTQRHVGWLWWSGSFNSARYSHTMPPNSKLCVYGGDDNYGAEAYGPLSR
                     HAGGVNVGLADGSVKFISQNINPATWWAMGTRAGGEVISSDQY"
     gene            9012..>9743
                     /locus_tag="VHS97_12245"
     CDS             9012..>9743
                     /locus_tag="VHS97_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449019.1"
                     /translation="MKLWRTATIAISWAVCLMIVVTTASPSSALETPGPAPAAALYVA
                     SDFPTLSRGFDPGLADEVIVRVWAPAQDEWRVTFSDETVTLHLRSKPGDAIPCWQSLG
                     TVKLAKGRSIKVIASDDRPPSQDRTASAGSSEMALLQGERTSKPVRPAIRLAPVPALL
                     WLASVPQSEAGPALDMIRGRIESVEPPPDSRRTQARTNRQGADFQAPATAQEWRDRAQ
                     HLREQMLVALGLWPMFPKTPLRPQVY"
BASE COUNT         2039 a         2901 c         2841 g         1962 t
ORIGIN      
        1 agcggaggcg aagtcgtggt cacggtcatg atcggattcc cctcatttcc tgggaatatc
       61 aaaccaatca cccggttgca ggacgaccgg ctgagcggca gtttcgttgg tcactcgtgt
      121 agcccacgca gcggcgtctt gcgcaattag cggaaacgtg ttgtaatgaa tgggcaagac
      181 gaagcgtggc tgaagcatct tgactgaacg cagggcgtcg tcaggaccca ttgtaaaata
      241 atcgccgatc ggcaagatgg cgaggtcaag cccttcctcg ccgatcaact tcatgtcgcc
      301 aaagagcgca gtgtcagcgg cgtcgtagat cttggttccg tccgggaagg tcaaaacgaa
      361 tccgcaggga ttgccgccgt tgctgccgtc ggggagcgct gagccgtgga aggcaagcgt
      421 cagcttgact cgcacggatt gatcgaaaac gaaactgcca ccgtgctgta agccgtgaac
      481 tttcggtccg ctcagacctt gggcgggctt ttgaagccac tcggagatct cgtagttggt
      541 ggcgactgtc gcgccggttc gctgggcgat cgcgactgca tcgcctacgt ggtctccatg
      601 gccgtgcgag atcaggatca agtcggccgg taccagttcg gcactgacca cggccttggg
      661 attgcctgtc aggaacgggt cgattagaac gtttcggccg tcgctttcga gaagaagggc
      721 agcatgccct agccagcgca cccgtgtgga catggtggtc tctcgctcga atgagccaag
      781 taaagtccga tactcccaac caaaatcatc caacagccgc gcatgcttga caatccaggt
      841 gttggctgga aacaaacgtt gagcgaaaag agcgagatcg gttgaccttt gcggtgattg
      901 ccggaaagat ggtgaagcct cactttcgtc acgctgaaat gaaatcgctc attccataaa
      961 ataagatcta agaagaactt cgggtttcac gtgccccaac catgatggtc agggagtaat
     1021 ccacaatgag ccagccaatc gatgtggaca gcccacggaa ggaccatgtc gtggggggcc
     1081 aagagcacac tgccccggta ggcgaaccag ggtcgggagg ttgttcccag tgtcaggccc
     1141 ttcaagcacg gatcgcggag ctcgaagcca ggttagaagg gcgatcggaa gttgggtcgg
     1201 aagcgatgtc ttcgcccggt ccagcgcttt cgtgcctcga attcaaggct cttcgcctac
     1261 ttccctgggg atggggggaa ggatggcaac tacgcccatc gccagcgcgc cgacactgga
     1321 tggatctgtc cccacacgct tataagtgct tacccatggt cattgccaac cagtggggtt
     1381 ggcagatcct gtgtcccacc gaagtcacgg taacctggga cggaacgcca aatccctccg
     1441 gcgtcagcgt cgaggtcgct cctcagtacc gcccagctat caagagccag tttggatcgg
     1501 gaatcgtcac gttctcgccc ccgtggctct tccgcacccc gagtggttgg gatttgtacc
     1561 ttaaaggtcc aagcaatcgt tggaaagaca actgcgtccc gctggaagga gtgatcgaga
     1621 cctggtggct gaattacacg ttcaccctca attggaagct agtccagccg ggaacggtga
     1681 ccttcgctga aggtgagagc ctgggccagc tcgtacccgt tccacatgcc accttcctgg
     1741 acgccacggc tctcgaggcg ccgatcggcc tgattgaacc gcgtgcggcc gaggagctct
     1801 tagaatggca agaaaaacgg cgacaattag cgcagcaggc ggtcactgtg catcaccttt
     1861 atcggaaagc ggagggaatc gacgagcatc tccagcgcgt tccggttccg ccggtcaagc
     1921 cgattgaacc atggaatcat gccccacgcg aagcttcgcc tgacgggccc actcccgccg
     1981 gttgatggag cgccgcggac gtgaaagaac cgaaggttgc acgaaccgtt cgaaggtagc
     2041 gaagattgca agcggattcg tcgtgcaatc ttcgccacct tcgtgcaatc gtcgaacatc
     2101 cgactgtcta tccaaaccgc tccagttctt cccctttggc aacgacgatg atgccgtctt
     2161 cggtgaccgt gaagccgcgg gcgaggtcgg cgtcgcgatt ccagccaatc tcaaagccca
     2221 tggggatccg gacatccttg tcgatgatcg cgcggcgaat gcgcgcgttg cggccgacgt
     2281 ggacaccgtc gaagagtatg gcgtcttcga tcaaggcgaa actgttgact cgcacgccag
     2341 gcgatacgat gcttctatac acctgtccac cggaaataat cgccccctga cagacgatcg
     2401 aattgtaggc ggctccccga cggtccccct cggtgtggac gaatttggga ggcgggtagg
     2461 ggggctgatg ggtatgaatc ggccagtcct ggtcgtagag attcaaaatc gggtcgatct
     2521 ggatcaagtc catggtggtc tggaagtaag catcaagggt accgacatcg cgccagtatg
     2581 cggcctcttt gcggttctcg tcgcggaacg ggtacgcaaa gacgcgtgag ccccccgcga
     2641 tcattgccgg gatgatgtcg cggccgaagt cgtggttact cgcctccttt ctggcggcgt
     2701 cctgaaataa cagctcatac atcacgtccg tattgaacac gtagatgccc atcgaggcca
     2761 gaaccatgtc gggctggtcg ggcatgacct gcggatcctt gggcttctcg agaaaggcca
     2821 tgacccggtg gtcgccttcc accccgatca cgccaaattc gcgcgactga tggcgcggca
     2881 caggctggca ggcaatcgtc agatctgccc ggttgtcacg atggtagtcg atcatccggg
     2941 cgtaattcat cttgtaaatg tggtcaccgg ccaggatcag cgtatcgcga ggcgcggctt
     3001 tttcgatggt gtatacgttt tggtaaatcg cgtcggcggt cccttgatac cacatctcgg
     3061 cgatccgttg ctggggaggg atcacctcga tgtattcgtt cagctcgcgc gacagaaacc
     3121 tccacccttg gtcaagatgc cgattcaggc tggttgcctt gtactgggtc agcacgagaa
     3181 tcttacgaat gtcggagttg atgcagttag aaagcgtgaa atcgatgatg cggtaaatgc
     3241 caccgaaagg gacagccggc ttggctcgat cgcgagtcag aggctccaaa cgcgttcctc
     3301 ggccaccggc caatacgatg gccaacacgt cgcgatacac cggcagacct ccgctcgacc
     3361 ctacgtcagg ggatggaaaa cggcctattg aagcacggat cttttcacca ttgtgcgcgc
     3421 gaagcgcacg caagccaacc gtttcgctcg aaggaccgta cacacggtga gggggtttca
     3481 tgcccatccc aacgttgctt gcaccgccag ctcagcgccg atccttatca aactgggcaa
     3541 cttccgagat agagacgagc aaggccggtg ccggatcgtt tcatttcttc taggtctcct
     3601 ggtagagtcg tcatggaacg cagtgttcaa cgaatttgct cgacacaaac cgatgttcgg
     3661 aatcaaagag caacgggagt gacctgaacc ttttcgggag gcgacgcggt gatggttggt
     3721 atgtctggtt ccgggccttt gctgacaatg ctcgcagttc ggttgagtat ccgaatcaac
     3781 caaatcgctg cttcgcttcg ctctcgggaa atggtggcgg tcggtttgta cgtcatactg
     3841 ataggattac cgggccgtgc ggccggggag aatcattccg atcgactcaa agaacttcag
     3901 acgtcttacg tcgtcagtgc cagccagaag atgccccgcg cctatcattt cgggtcgcaa
     3961 gaaccagctg atgtcttctc aaaccacgcc agccacacca accggttggt tccggtctac
     4021 gtcttcggag ggaaatccgc gctcagcgcg gtcaccggcg ccaacagcaa gtatcgggat
     4081 ccggagaaga tcaaggctct ctatggtttt ctgccccagc acaccgtgaa cccgcaggcc
     4141 gactatgccg accagagtga cctgtattgg gttcaaaagg aggcgatatc acgaggagtc
     4201 aagcacctat tcatcgtctg gttcgacggg cttgactggc cgacaacaca ggccgccgcg
     4261 attgtaaaaa ctggcagagt ctacaccgag gggaagggct cgggcctgat cttccaggat
     4321 tatgacgccg gcggcagagc gcagtatggt ttcgtggtca caagcccgac gcatgagcaa
     4381 aatgtgccga atctcgacgc gcaaactgtc gtgatcccac cggacagctt gggtggcggc
     4441 tacgatgcgc ggatcgccgg ttcagagccc tggtcactcg gcccgctggg actgaaggcg
     4501 cggggctact tgaagggcca gtcggcgagc gatgccgatc gggcgggcgt acttgccgtc
     4561 ggcggcgtcc ttcacgcgta caccgattca tcgacgagtg ccgcggagat ggtcagtggt
     4621 gttaagtcgt acaacaacgg tctcaatgtc accgatgacg gccggctcgt gacaactctt
     4681 tttcaccagc tccaggacca agggtggaaa gtcggcacgg tcaccagcgt cccttttaac
     4741 cacgcttcgc ctgcggcaat gtacacgcaa gacgtttatc gcgacgacta tcaggacctc
     4801 gcccgtgaga tgctcgggtt gccgggaatc cttcagcagg ctcgccgcgt cctgctccgg
     4861 ccgggcctcg acgtggtcat gggtaccggc ttcggcgtga tcaccacccc gaagtctctc
     4921 gccgcacaag gaaccaacgg cgtgcttgga agcttgttca tcaccgacgc tgacctcgca
     4981 gcggtcgacg tcaaaaacgg cggcaagtac attgtgtctc ataccgagcc aggcgccagt
     5041 ggcggccagt tgttgaaacg tgcagcaacc gaggcagccc ggcgctccgc gcggctgttt
     5101 ggctttttcg gccgccccgg gctcgatcat ctcccctacc agaccgccga cggccactac
     5161 gatcctgccc ccagtctcaa tagcagcggc aagctcagcc cagccgaatc gtacagcccg
     5221 gctgatctca ccgaacaacc gaccctggcg caaatgaccg aggctgcctt gaccgcgctc
     5281 ggaggacagc ccagccagcc gtttgctctc tttatcgaag ccggcgacgt cgatttcgcg
     5341 ctccacgcca acaatctcga taatgcgatc ggggctgtct atagcggcga agaagcaatt
     5401 cgcaccgtca tccgatgggt tgagacccac agcaactggg acgactcact catgatcgtt
     5461 tcgtcagacc acggacatta cttggtcgtc gacgatcctc aggcactggc cgcggccaaa
     5521 caagccaagt gacctattga acaagaataa gctcgaaatc agaggctgcc gtgatgatca
     5581 ctcaacattg ccgcactcgc cctttggttg ctccactggc gttcgcgatt gcgctattct
     5641 gcggctctgc cggttccggc agctcgcgcc agtcgagcga gccgcctgct accggagcgg
     5701 tccgtcactg cccgcagctc ctccagccgg gtgatccgca cacccatggt ctgagcgatg
     5761 ccgacctcga atcactgagc gcagttttgc gcacggccgt cgataatcac acagtgcctg
     5821 gtgtaacgct tttgctggct cacaagggcg aagtgatctt caagaagtcg ttcgggaacc
     5881 tcgctgtcga ccacaaggcg ctgattgcct cgagctccaa gcccgtcacc gcgacgatcc
     5941 tcatgatcct ggtcgaccag ggaaagctct cactcgacga cccggtcgag aaatacctgc
     6001 cggaatttaa gggaattgcc atcaaccgcc gcccaccggc gaagctgcca accgttcgac
     6061 acctgctctg caatatgtcg ggactgcccg gcgacttcct cgccgaatca cttctcaaac
     6121 gcttaagaaa cggagctggc aacgctcagg atgaaatcaa ggggaaggaa cttgagaagg
     6181 ttaactcgtc gggccgtttc gccgccggag gaaaccgctc gctggccgat tcagtgcgcg
     6241 agctggtgaa ggggggcttg gcgaccgagc ccggagccga gtttcactat tgcacgatgg
     6301 gtttcaacgt cgcggcccga gtggccgaag tcgcggtcaa acgaccgttc gaggagctga
     6361 gccgaacaga gctgctcgag ccgatgggca tgaagaacac tcgctacact ccgtttggct
     6421 tgcaggcact cagttcccgg ccgacgctct cgaatggcga gagccgattc atcatggccg
     6481 gtggcggcat gacctcgaca ctcgacgact tcgccgcatt ctatcagatg catttgaacg
     6541 gtgggacgta tcgtgaccgg cgaatcctat ctgagcaggc ggtggcgacg atgcacacgc
     6601 gacaggccaa gctcgagttg ctgatgtccg ggccctacgg caacgactac ggcctagcct
     6661 tcttcctcga tcggcttgat gaaaagggag aagcgcgcgt tattacccac cctggtctct
     6721 ttggcaccac cccctggttc gacaaggatc gcgatttggt gggaatactg ttcgtccagt
     6781 ccaatttcct gcgagtgacg tcgttggtcc acgaagtcca ggcgaagctg cgtgccatct
     6841 gcaagcgttc gccgtaagaa cctgccttct cgggaacttc atccgtcctg ctgaccagac
     6901 gcagacgcgg accaacgacg tagtctgcaa tgccactcac acggttgcca ccgcgactcc
     6961 ttccggtaac gtgacgcgtt acgccgatcc tcggctcgag accggcacaa cttccccgcc
     7021 ggccatcaag catggtctca ttctatcgcg gcgtaaaaga cctgcgagca tttcgatata
     7081 atttgatgaa gaaccagtaa gagcgttgac gctcagcggg catcctccta tgattcaaac
     7141 cgcgactgac cccacggtgt gagaaccacc atcccgccat ctgtgcatat tctcacttaa
     7201 gtgcacacga cgaggaatcc tgacgatgca acattggcgc ggccagttct ggcttttcgt
     7261 tttcctcgcg gtcgtttggc cgggttgcgg ccttagtgac ggagcgccgc cagtctcgtc
     7321 gtcgctcgag gaggcgacag tcaagggaac cgtacgggtt cgaagcaaga ccgtcaacaa
     7381 cggccaaatc gcttttcatt ctgctaacgt cagcaggccg aacgctgcca tccgtgaagt
     7441 ccccatagcc aaggatggga cctactcgat tacgacgctg gtcggcgcca acaccgtgga
     7501 agtcgactgc aacgaactgc gtgacccaaa gacgcgtcgc cgcctccaac acaacctcgc
     7561 cttcgatgtt caggcaggcg aaaacacttt cgacatcgac ataaccggga tggcgcccgc
     7621 accgaccagg tgagacagct ttagttcgtc ggcaatgcgg tcggtcggta cacaggtttg
     7681 tcatccccgc gcaacctagc cgttgcggct tcggttcgga agtcattacc tacgtagccc
     7741 caattctcgc aggagtacgt gatgaaaagg ataggtcctc ggtccggatt tacgctcatc
     7801 gaattgctcg tcgtcatcgc gatcatcgct gtgctgattg ctctcgtgct accggcggtg
     7861 caagcagccc cggaggcggc gcggcgcctc ctgtgcgtca acaatctgaa acaaatcagt
     7921 ctggcatctc attcatatgc gaacacatat ggttcgtttc cgatcggtca cgggcccgta
     7981 aattacaacg attggggagg gacggcgttc attcttccgt tcgccgaaca gacgcccctc
     8041 tacaattcca tcaattttgt ctacggcggg gccaatccgg ttggcgtcaa acttcctaat
     8101 ccagtcctgg gcgtcgggtc tcgggtcaac atcaactcga ccgtatttgg gacttacatc
     8161 aatatgtaca actgcccatc agatggtcgc gacgcctaca cctactcgtc gggcgtcggc
     8221 tacaatccac ctcactttaa ctacaccgcg agcgcaggcg cgatccccct cgacgactgg
     8281 ctcttctacg gaggcacgag tcagtgcgac ggcgtctatt gccatgtcga cggctcgtcg
     8341 gtccagccgt atgcaactgc tctcggagct ccgaccgggt tcgtcgtgtc agtcgcgatg
     8401 atcaccgacg ggctcagcaa caccgcggct tggagcgagc gcgtccgcgg aatcggcttc
     8461 gcggaaaacg gtctggtcga ttctcagcct ccttcgacaa ctctgtggta tatccctccg
     8521 ttcactcaag gcggcagcct tgccgacgtt cccatcgtgt ataacaattg catcgcttcg
     8581 actaccgtct attcgtcggc cgtgtcgccg tcgggctcaa ctcaacgtca cgtaggttgg
     8641 ctgtggtgga gcggttcgtt caattcggcg cgctatagcc acacgatgcc gcctaactcg
     8701 aagctatgcg tctacggcgg ggacgacaac tatggtgccg aggcgtatgg tcccttgagc
     8761 cggcatgccg gcggagtaaa cgtcggcctc gcagacggct cggtcaaatt tatcagtcaa
     8821 aacattaatc cggcgacgtg gtgggcgatg ggaaccaggg cagggggaga ggtcatatcg
     8881 agcgaccaat attgacctcg aacttgcctc ggtcggcagg attgtgcgtg accagcgctg
     8941 cgggcctccg aattgggagg ggggctcgaa gtgcccaagc gtacgctcgg tcaaacatgg
     9001 agttacaagc catgaagctt tggcgaactg cgacaatcgc gatttcttgg gcggtctgcc
     9061 tgatgatcgt cgtcaccacg gcctccccga gctccgcact ggagacgcca ggcccggcac
     9121 ctgctgcggc tctgtacgtg gcaagcgact ttccgacttt gtctagaggg tttgatccgg
     9181 ggctggcaga cgaagtgatt gtgcgcgtgt gggccccggc gcaggacgag tggcgcgtga
     9241 cgttcagtga cgaaaccgtg actcttcatc tccgcagcaa gccaggcgac gcaattccgt
     9301 gctggcagtc gctcggcacg gtcaaacttg cgaaaggccg gtcgatcaaa gtgatcgcct
     9361 ctgatgaccg gcccccaagc caggaccgga ccgcttcggc cggatcgagc gagatggcat
     9421 tgttgcaagg agaacggact tcaaagcccg tgagaccggc gatacgcctt gctcccgtgc
     9481 ctgcgctcct ctggttggcg tcggtcccgc agtccgaagc gggaccggct ctggacatga
     9541 ttcgaggacg gatcgaaagc gttgagccgc cacccgactc gcgccggacg caggcccgca
     9601 caaatcgtca gggagccgac ttccaggcgc cagcgacggc ccaagaatgg cgagaccgtg
     9661 cccaacatct ccgtgaacag atgctggtgg cgctcggact gtggccgatg tttccaaaga
     9721 ctccactccg cccgcaggta tac
//
LOCUS       DATAQL010000702         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5416412, whole genome shotgun sequence.
ACCESSION   DATAQL010000702 DATAQL010000000
VERSION     DATAQL010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5416412"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1265)
                     /locus_tag="VHS97_12250"
     CDS             complement(join(139..827,827..1265))
                     /locus_tag="VHS97_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611506.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3449020.1"
                     /translation="MEKYRVTLQAAERTALERLISAGKTASRKLTHARILLLADAVHG
                     EGARDEDIVAALSASLRTIARVRKRFVTEGIEAALDPRPRPARPDKIKIKGNVEQKLV
                     ELACTDPPRGRCHWTLQLLADEMVVLGLVDSISTETVRLALKKTNIQPWIVQSWCIPP
                     KANAEFVWHMEDVIQTYMLPYDPKWPVVCFDEASKQLFGEVRAPESARPGAPARVDYE
                     YERKGVCCQLVMCEPLRGWRHVKVTERRTRQDYAHCIREMVDQYFARATKIRLVQDNL
                     NTHDGASLYEAFPPAEARRLLDKIEFHYTPKHGSWLNMAETEINIMNRQCLDRRLDNQ
                     SLMASEIAHWEDERNKRKARIHWTFTLAVARQKLRKLYPSI"
     gene            complement(1340..>1684)
                     /locus_tag="VHS97_12255"
     CDS             complement(1340..>1684)
                     /locus_tag="VHS97_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449021.1"
                     /translation="QSAEAVSAVEKAIALDDTSVASRATAARLYEASGRFADAAAGYR
                     KLATIDRRARTEYLTNVAKLEARLGRRDEALKAGRDLLAAAPGNPEHFQFFSDLCFQL
                     GENEEGLVSGQS"
BASE COUNT          292 a          537 c          509 g          346 t
ORIGIN      
        1 aaaacacatc agcaaacgct ttccccgaac gaagccaatc acggttgcca gtttccagtt
       61 cccggaacac ggggctgcct caagccccag gccgcccggt ctggcaacta gcaactagca
      121 actagtgggc cgacaatctt agattgacgg gtaaagtttc cggagtttct gacgggcgac
      181 ggcgagcgtg aaggtccagt ggatccgtgc cttccgcttg tttcgctcgt cttcccagtg
      241 tgcgatttcc gatgccatca ggctctgatt gtcgagacgc cggtccaggc attggcggtt
      301 catgatgttg atctctgtct ccgccatatt cagccaacta ccgtgcttgg gggtgtagtg
      361 gaactcgatc ttgtccaaga gccgacgtgc ctctgcgggt gggaacgcct catacaagct
      421 cgccccatcg tgggtattga ggttgtcctg caccagccgg atcttcgtcg cccgtgcgaa
      481 atactggtcc accatctcgc ggatgcagtg ggcgtagtcc tggcgagtgc gtcgttcggt
      541 caccttgacg tgacgccacc ctcgcaacgg ttcgcacatc acgagttggc aacaaactcc
      601 cttcctttcg tactcatagt cgactcgggc gggcgcgcca ggacgagccg attcgggcgc
      661 ccgcacctcg ccgaacagtt gcttgctcgc ctcgtcgaag cagaccaccg gccacttcgg
      721 gtcgtagggg agcatgtagg tttggatcac gtcctccata tgccagacga actcggcgtt
      781 ggccttcggg ggaatgcacc atgactggac gatccagggt tggatgtcgt ttttttcagc
      841 gccaagcgga ctgtctcggt gctgatcgag tcgaccaagc ccaagacgac catctcgtca
      901 gcgagcaact gaagggtcca gtgacaccgg ccgcgcggcg ggtcggtgca tgccagctcg
      961 acgagtttct gttcgacgtt ccctttgatc ttgattttgt ccggccgagc cggccgtggc
     1021 ctaggatcga gggccgcctc gattccttcg gtcacgaacc gcttcctcac tcgagcgatg
     1081 gtccgaagac ttgcgctcaa ggcagcgacg atatcctcgt cgcgggcacc ctcgccatgg
     1141 acggcatccg ccaggagcaa gatgcgcgcg tgggtcagtt tgcggctcgc ggtcttcccc
     1201 gcagagatca agcgttccag tgccgtccgc tccgcagcct gcaacgtaac ccggtatttt
     1261 tccatcgcgg tgctccgtca cctgagggcc atccatcaac cgatgcccca ggagacagga
     1321 gtccgcttca cccgtcaatc taagattgtc cgctcactag cccttcctcg ttctcaccga
     1381 gctggaagca gaggtcggag aagaactgga agtgctcggg gttgccgggt gcggcggcga
     1441 gcaggtcgcg accggcctta agggcctcgt cgcgacggcc gaggcgggct tcgagcttgg
     1501 cgacgttcgt caggtattcg gtcctggccc tccggtcgat cgtcgcgagc ttgcggtaac
     1561 cggcggccgc gtcggcgaac cggcccgacg cctcgtagag ccgagccgcc gtggcccgcg
     1621 acgcaacgga cgtatcgtcg agcgcgatgg ccttctcgac cgccgacacc gcctccgccg
     1681 attg
//
LOCUS       DATAQL010000703         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4804843, whole genome shotgun sequence.
ACCESSION   DATAQL010000703 DATAQL010000000
VERSION     DATAQL010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4804843"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VHS97_12260"
     CDS             <1..1028
                     /locus_tag="VHS97_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX3449022.1"
                     /translation="AEPGEFTLRAFLSGRIDLTRAEAVLGVIEAQNPAQLDAALEQLA
                     GGLSGPIMAMRNHLLDVVAHLEANLDFTEERDVDPLGRAALAGELEHAAALLEVFERR
                     LTDRDRPDGRPCVVLLGPPNVGKSRLYNALLGDGRAIVSPQAGTTRDYLAALCDCDGL
                     TVELVDTAGVEAAGDSITAQAQALRGLQVDRADLILVCRAADTPHPVAVEPASRCHKL
                     MVWTKCDQGLPDRGDDGYARDPIVTSAATGFGLEALRSAIARTLRYQKTEGNLPAGTG
                     ARCRGSLRRAREALGQATECLTRGGGEELVAFDLRSAVEELGKVVGAVVTDDILDRIF
                     RRFCIGK"
     gene            1139..2143
                     /locus_tag="VHS97_12265"
     CDS             1139..2143
                     /locus_tag="VHS97_12265"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX3449023.1"
                     /translation="MANEFRIETDTIGEVQVPSDRLWGAQTQRSHENFKIGVERMPLE
                     LIHALALVKRAAAQVNLDLGKYKDPTIGQSIIMAADEVKSGKHDVEFPLVVWQTGSGT
                     QTNMNVNEVIANRASELMGGERGMGRKVHPNDHVNMGQSSNDVFPTAMHVAAVTALRD
                     RLIPAVRLLRTTLAQKAATYRDVIKIGRTHLMDATPLTLGQEISGWVHLLDNGLVHTD
                     EVMPHLCELALGGTAVGTGLNAHPEFAVRVAAELSRLTGYPFVTAPNKFEALAGHDAL
                     LFAHGALKTLAASLMKIANDVRWMASGPRSGLGEIFIPENEPGSSIMPGKVNPTQCEA
                     "
     gene            2146..2547
                     /locus_tag="VHS97_12270"
     CDS             2146..2547
                     /locus_tag="VHS97_12270"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449024.1"
                     /translation="MVASQVMGNDVAINFGGALGNFELNVMKPLIIHNFLASVRLLAD
                     ACVSFNDHCAAGIEPNRKRIDDLLKQSLMLVTALNPHIGYDNAAKIAKNAHKKGITLR
                     ESAIELKLLSPDQFDAWVRPEDMVGQEKRSS"
     gene            2617..>2813
                     /locus_tag="VHS97_12275"
     CDS             2617..>2813
                     /locus_tag="VHS97_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449025.1"
                     /translation="MIRHGTFALVILATVFLTNPVNGQTRYRQPSADIVAILDAPPPP
                     RAIESPTRDALLLVEMKPYPS"
BASE COUNT          556 a          871 c          875 g          511 t
ORIGIN      
        1 ccgccgagcc tggggagttc acgctcagag ccttcctctc cggccgcatc gacctcacgc
       61 gggccgaggc ggtcctgggc gtgatcgaag cgcaaaaccc ggcgcagttg gacgcggccc
      121 tcgaacagct tgccgggggg ctttccgggc cgatcatggc catgcgtaat catttgctcg
      181 acgtggtggc gcatcttgag gccaacctgg attttaccga ggagcgtgac gtcgatccct
      241 tgggtcgagc ggccctggcg ggcgaactcg agcacgcggc cgccctgctg gaagtgttcg
      301 aacggcgact gaccgaccgc gaccgccccg acggccgccc gtgcgtggtc ctacttggtc
      361 ctcccaatgt gggaaaaagc cgactctata acgccttgct cggcgacggc cgtgccatcg
      421 tctcgcccca ggcgggaaca acccgcgatt atctcgcggc tctctgcgat tgcgacggcc
      481 tgaccgtcga gctggttgat accgctggcg tggaagcggc cggcgattcc atcaccgcgc
      541 aggcccaggc cctgcgcggc cttcaagtcg accgcgccga tctgatactc gtgtgtcgcg
      601 cggccgatac gccacatcct gttgcggtcg aacccgcgtc ccgttgtcat aagctcatgg
      661 tctggaccaa gtgcgatcag ggcttgcccg atcgtggcga cgacggctat gcgcgcgatc
      721 cgatcgtcac aagcgctgct acggggttcg gtctggaggc gctgcgatcg gcaatcgcac
      781 gaacgcttcg ttaccagaaa accgagggaa acttgcctgc cggcactggg gcgcggtgtc
      841 ggggcagcct caggcgggct cgcgaggcgc tagggcaagc gaccgaatgc cttacacgtg
      901 gaggcggaga ggaactggtg gccttcgacc tgcgatcggc cgtcgaagag ctgggcaagg
      961 tcgtcggcgc cgtggtaacc gacgacattc tcgaccgaat tttccgacgg ttctgcattg
     1021 gaaaatgagg gcatggtaaa gttggcgaac ggcgaggcat gccgatgcgt cgcgcacggc
     1081 gagacggtat cgtataagtg ccaatcctcg cgaagtcgct ctattgaggt gtacagcgat
     1141 ggccaacgag tttcgcattg aaaccgacac gattggggaa gtccaggtgc catctgatcg
     1201 gctctggggg gcgcaaaccc agcgctcgca cgagaacttc aagatcggcg tggaacggat
     1261 gccactggag cttatccatg cacttgcgct cgtgaaacgc gcagcggcgc aggtcaatct
     1321 cgacctcgga aaatacaagg atcctacgat cggccagtca atcatcatgg cggcggacga
     1381 ggtgaagtcc ggcaagcacg atgtcgagtt tcccctggtc gtctggcaaa ccggcagcgg
     1441 cacgcagacg aacatgaacg tgaacgaggt gattgccaac cgagccagcg agcttatggg
     1501 aggcgagcgc ggcatgggcc gcaaggtgca ccccaacgac catgtcaaca tggggcagtc
     1561 gtcgaatgat gtgtttccga cggccatgca cgtggccgcc gtgacggcgt tacgcgatcg
     1621 tctgatacct gccgtgcgac tgctgcgcac gaccctggcg caaaaagccg cgacttatcg
     1681 ggacgtgatc aagatcggcc gaacgcatct gatggacgcg accccactga ccttaggaca
     1741 ggagatctcc ggttgggtgc atcttctcga taatggtctt gtccacactg acgaggtgat
     1801 gccccacctc tgcgagctgg cactgggagg cacggcggtg ggtacgggct tgaatgccca
     1861 ccctgagttt gcggtccgtg tggccgccga gctgtcccgc ttgacgggct atccatttgt
     1921 gaccgcaccc aacaagttcg aggcgctcgc cggacacgat gccctcttgt ttgcgcacgg
     1981 cgcgctcaag acgcttgccg cctcgctcat gaagatcgcc aacgacgttc gctggatggc
     2041 gtcgggaccg cgaagcggct tgggggagat cttcatcccc gaaaatgagc cgggcagctc
     2101 gatcatgcct ggaaaggtga atccgactca gtgcgaggca tgacgatggt ggccagccag
     2161 gtcatgggca acgacgtggc catcaacttt gggggcgcgc tcggcaactt cgagttaaac
     2221 gtaatgaagc ccctcatcat ccataacttc ctggcgagcg tccgactgct ggccgacgcc
     2281 tgcgtcagct tcaacgatca ttgcgcggcc ggcatcgaac cgaaccgcaa gcgaatcgat
     2341 gatctcctca agcagtccct gatgctggtg acggcactca acccccatat cggctacgac
     2401 aacgcggcca agatcgccaa gaacgctcac aagaagggga tcacactccg tgagtcggcc
     2461 atcgagctca aactgctcag ccccgaccag ttcgacgcgt gggttcgtcc cgaagatatg
     2521 gtgggacagg agaaacgatc gtcttgatct taaactttct cgagtgcgca agccgcacgt
     2581 gcgcgtcgtc ccatccacaa gcaagaaaga accgccatga tccgccacgg cacgttcgct
     2641 ctcgtgatcc tcgcgaccgt ttttttgacc aaccccgtga atgggcagac acgctaccgg
     2701 caaccatcgg ccgacatcgt cgccatcctt gatgcgcctc ctcctccgag ggcgatcgag
     2761 agccccacgc gcgacgctct gttgctggtc gagatgaagc cttatccatc gat
//
LOCUS       DATAQL010000704         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1598312, whole genome shotgun sequence.
ACCESSION   DATAQL010000704 DATAQL010000000
VERSION     DATAQL010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598312"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VHS97_12280"
     CDS             <1..1542
                     /locus_tag="VHS97_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3449026.1"
                     /translation="RVFRHFVQISFPVALGSVAILGARPWVGDWIKQSRENARPLPPP
                     GSPNVVFIVLDTVAAGHLSLLGYDRATSTSLIELAEQGIRFDSARTAAPWTLPSHATM
                     FTGRWMHELSVGWLTPLDNTHPTLAEFLSARGYATAGFVANSTYAGSDSGLGRGFTHF
                     EDYSFPGLTALKTAVLVDRVMLATQSMTDFLEDRQELIRWRPYVQHLLGLFLSDRKAA
                     ATVNRELLEWLTRRAQPERPFFVFLNYIDAHYPYLLPSGRLHRFGGAPTDLGQRTMLQ
                     HWADLDKNLLSPQDLAFAGKAYDDCIADLDEQVGKLVDKLRRRGILERTWLIVVSDHG
                     ESFGEHAGIFCHGSSLYQTELHVPLLIVPPEGSGTKRVVTDTVSLRDLPATVVDLLDL
                     REGSPFPGSSLTRFWQGASPTPVPPPSSDHALAEVVPDDPHNRDSSGLPKKTWPLGAL
                     NEGEWSYIRRENDLREELFHLREDAQEQHNLATLPTARSRLEQMRQSMSQLTGGPLLP
                     HRFNR"
BASE COUNT          371 a          578 c          553 g          411 t
ORIGIN      
        1 cgcgtttttc ggcatttcgt gcagatcagt ttcccggtgg ccctggggtc cgtcgcgatt
       61 ctgggtgcga gaccctgggt gggcgactgg attaagcaat cgcgtgagaa tgcgcgcccc
      121 ttgccgccgc cggggtcgcc caatgtcgtc ttcatcgttc tggacacagt cgcggccggc
      181 catttgagcc tccttggata cgaccgcgcc accagtacgt ccttgatcga gcttgccgag
      241 caaggtatca ggttcgattc tgctcggacg gcggcgccat ggacgttgcc gtcgcatgcg
      301 acgatgttta cggggcgatg gatgcacgag ctctcggtcg gttggcttac accgctcgac
      361 aacactcatc ccacgctggc cgagtttctc tcggcgcggg gctacgcgac ggcaggcttc
      421 gtggccaact ccacctatgc cggcagtgat tcgggcctgg gccgcggctt cactcatttc
      481 gaggactaca gctttcccgg gctcactgcg ctcaagacag ctgtcctggt cgatcgagtt
      541 atgttggcga cccagtcaat gacagatttc ctggaggatc gccaggaact tattcgttgg
      601 cgaccctatg tgcaacacct cttggggctg ttcctcagcg atcgcaaggc ggcggcgacg
      661 gtcaatcgcg aactcctcga atggttgacg cggcgggcgc agcccgagcg gccttttttt
      721 gtgttcttga actacattga cgcccattac ccctatttac tgccgtcggg gagattgcat
      781 cgttttggcg gcgcacccac ggacttgggc caacggacga tgctccagca ctgggcggat
      841 ctggacaaga atctcctgtc gccgcaagac ctggcatttg ctggtaaagc ctacgacgat
      901 tgtatcgcag accttgatga gcaggtcggc aagctggtgg ataagctgcg gcgccgcggg
      961 attctagagc gaacctggtt gatcgtcgtt tccgaccatg gtgagagttt cggcgagcac
     1021 gcgggcattt tctgccacgg gtcgagtctc tatcagaccg agttgcacgt tccacttttg
     1081 atcgttccgc ccgaaggaag cgggaccaaa cgggtggtta cagatacggt gagtctgcgt
     1141 gacctgccgg cgacggttgt cgacctgctg gatctgcgtg agggctcgcc gttccccgga
     1201 tcttcattaa cccgcttttg gcaaggggca tcaccgacac cagtccctcc cccatcatcg
     1261 gatcatgcgc tcgccgaggt agtcccggac gacccgcaca atcgcgattc ctcgggtttg
     1321 cccaaaaaga cctggcctct cggggccctg aacgaaggag aatggtcgta tatccgccgc
     1381 gaaaacgacc tccgcgagga gttgttccac ttgcgcgaag atgctcagga acaacacaat
     1441 ctcgctaccc ttccgactgc gcggtcgagg ctcgaacaaa tgcgacaatc catgagtcaa
     1501 cttactggcg gcccgctctt gccacatcgc ttcaatcgtt gacgcagcaa agtaatcgct
     1561 cgcaaggcga gccgcattgt tcaacgatgt ccgaactgcc ggccgacatc gtcggattta
     1621 ccgaagcaaa gaaccaaatt tcattgagca cgatgcggat aatctcctag atttatgtgg
     1681 gaaggaagag gtatcctagg tggtactgat aaaccgcaat cggccccgcc atcctgtacg
     1741 tcagtgcgca ttatgagcac ctattcgtga ccccccacca cgcttggctg gacggcagag
     1801 cgaccggctg ctaccagccc ttcgcggtcc cgggccgtgc caaagaggaa ttatcacatg
     1861 ctcacattca ccggcaaagg ccaaacatcg acctgctccg gcatgacccg gcg
//
LOCUS       DATAQL010000705         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_989179, whole genome shotgun sequence.
ACCESSION   DATAQL010000705 DATAQL010000000
VERSION     DATAQL010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989179"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VHS97_12285"
     CDS             <1..280
                     /locus_tag="VHS97_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449027.1"
                     /translation="RWVQWLLMAHARRYHRHYGTSGHVWQGRFKAFIIQDDDHLRTVL
                     RYVERNPLRAELVARAEDWKWFSPTSPAGFGVTRSSGEAAQKCATSRG"
     gene            complement(564..857)
                     /locus_tag="VHS97_12290"
     CDS             complement(564..857)
                     /locus_tag="VHS97_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449028.1"
                     /translation="MVYFLEGAGEAEKPEDPLDPAELGTVVRALALQAHKILRRSTTG
                     KFADLHVLHDQLDRLQMRLDQLRRSHALRFDELQRWLGQLSQETKDRDRECSG"
     gene            1118..1510
                     /locus_tag="VHS97_12295"
     CDS             1118..1510
                     /locus_tag="VHS97_12295"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3449029.1"
                     /translation="MPPDDCGSITRYLLISKESDDRILVDDAVRTLWERYYQKLVRLT
                     RDRLRNSPSGRQHVFAVTTADTLQWLLDALPNETLRTIATNKMEGYTNQEIARSLTCC
                     EATIELKLRHMRAVWLDLIQAESTESDA"
     gene            1507..>3068
                     /locus_tag="VHS97_12300"
     CDS             1507..>3068
                     /locus_tag="VHS97_12300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449030.1"
                     /translation="MTFDEFPPTIRITLLNVCDEFEAAWTSGRCPRIEDYLNAWTEPE
                     RTALLAMLVPIEIELRKNDAVPAALQEYQERFKKYALVIQDFYPGPVAPTAVEIPLTE
                     LGSSAQPPDRPPKTEPGTTIGYEPVHGDEIREPLASPLVSSRYRDLRHLGEGNFEVYR
                     AFDEQNGRYVAIKIARSNDHTSSAMMSLAHEADAYKALNHPRIVKLYEYVCAVDNEAG
                     GGEFIVMEYIEGQTLEQKLKDHVLSRSQIVEIVAKVADALCHAHNQKPGLIHRDLKPS
                     NILIDLEGEPHVCDFGLAINENVLWQRKGEIAGTYKYMAPEQVRRETNKFDGRTDIWA
                     LGVILYRGLTGAFPFSGPSNDAIIEQILHHDPKPLRMHDPDIDAELERICLRCLLRPM
                     AERYRIASDLSEELLAALDRPEPREPATSPLILPPKGFAPYDNEYKKFFLAMLPGPRG
                     ANGLPESLRYWKQWVESTDSDPAQPVGLLCGPSGGGKSSFVRAGLLPHLDRKLVYPIY
                     VEATRTGTEARLL"
BASE COUNT          666 a          922 c          872 g          608 t
ORIGIN      
        1 ccgatgggtg cagtggttgc tcatggccca cgcccgacgc tatcaccgcc actacggtac
       61 cagcgggcac gtttggcaag ggcgattcaa ggcgttcatc atccaggacg acgatcatct
      121 ccgaaccgtg ttgcgctacg ttgagcgtaa ccccttgcgg gccgaactgg tagcgcgggc
      181 ggaggactgg aagtggttca gcccaacctc cccggctggc ttcggcgtga cccgctcttc
      241 tggcgaggcg gcccaaaagt gcgcgacgag tcgtggctga aacgcgtcaa cgacccgcta
      301 tccgacggag acttgcgacg attgcgactc tcggtagagc gcgggcggcc ctttggcact
      361 gagccatgga cccgggagac ggcccggcgg ctggggctgg agtggactct gcgatcaaga
      421 ggcagaccgc ggaaaaaccc gttctaaatt tgttatgtcc ccttttgtgc ctggcaacgc
      481 attacccgga actcgtatcc ccggaaaccg gtagtgtccc cggaccccta tccggtctcg
      541 cacgacgacg ggtcgtcgat ccatcaaccc gagcactctc ggtcgcgatc ctttgtttct
      601 tgcgagagtt gcccaagcca tcgttgtaac tcatcgaacc gcagcgcgtg cgaccgccga
      661 agctggtcga ggcgcatctg gagacgatcg agctggtcgt gcagtacgtg caagtccgcg
      721 aactttcctg tcgtacttcg cctaaggatt ttatgcgcct gtaaggcaag cgcccgcacg
      781 accgtgccaa gctcggcggg atcgagtgga tcttcaggct tctccgcctc gccggctcct
      841 tcaaggaagt agaccatcgg ggcgatcggg attcgcgatt gaaacgggtg atgctaagta
      901 acggattggt ctatggcagg ggtttcttcg gtaaacgtct gggtcaagag gccggtcggc
      961 agtcgcggcc ggtttaccgt ctagtcccca ttcttaagca ttgcgcattt ccgttcgtac
     1021 ccctaacgct cgtgtgagat tcttcccttt tcctcgattt tcgttcagaa tgaacgtccg
     1081 cgatcaaaac acttcttaac tcgctacgag gatgctaatg ccgcccgacg attgcggctc
     1141 gatcactcga tacctgctta tctcaaagga aagcgatgat cgcatcctgg tggacgacgc
     1201 cgtacgtacc ctctgggaaa ggtattatca gaaactggtt cggctgacga gagaccgatt
     1261 gaggaattcg ccgagcggac gacagcatgt ttttgccgtg acaacagcag acactttaca
     1321 gtggctcctc gacgccctgc cgaacgaaac gttacggacg atcgccacca acaagatgga
     1381 ggggtacacg aatcaggaaa ttgcaagaag cctcacctgt tgtgaggcca ccatcgagct
     1441 caagctgagg catatgcggg cggtctggct cgaccttata caagccgaat ccacagagag
     1501 tgatgcatga catttgacga gtttccccct acgatccgca tcacgctcct caacgtttgc
     1561 gacgaattcg aggcggcctg gacgtcgggc agatgcccca ggatcgaaga ttacttgaac
     1621 gcgtggaccg agcccgaacg aaccgcgctg ctcgcgatgc tcgtgccgat cgagatcgaa
     1681 ctcagaaaga atgacgcggt gccggcagcg ctgcaagagt accaggaacg cttcaaaaag
     1741 tacgctcttg tcatccaaga tttctatcct ggccccgtcg cgccgaccgc ggtcgaaatc
     1801 ccgttgaccg aattgggttc ctctgcacaa cctcccgaca gaccgccgaa gactgagcct
     1861 ggaaccacca tcggctatga gccggtgcac ggggacgaga ttcgtgagcc actggcctcg
     1921 ccgctggtca gttcccggta tcgtgacctg cgccatctcg gcgagggcaa cttcgaggtc
     1981 taccgagctt tcgacgagca gaacggtcgc tacgtcgcga tcaagatcgc gcggtcaaac
     2041 gatcacacgt cgtcagcgat gatgtcgctc gcccacgagg ccgacgccta caaagccttg
     2101 aatcatccac ggatcgtcaa attgtatgaa tacgtatgcg ctgtggacaa tgaggccggc
     2161 ggcggcgaat tcatcgtgat ggaatacatt gaaggccaaa ctctggaaca gaaactcaaa
     2221 gatcacgtcc tgtcgcgcag ccaaatcgtc gagatcgtgg cgaaggtcgc cgacgctctt
     2281 tgccacgccc acaatcaaaa accaggactg atccacaggg acctcaaacc gtcgaacatc
     2341 ctgatcgacc ttgagggaga gcctcacgtt tgcgacttcg gccttgccat caacgagaat
     2401 gtcctctggc aaaggaaggg cgagatagca gggacttata agtacatggc gccagaacaa
     2461 gtccgccgtg agaccaacaa attcgacggt cgcaccgata tctgggcgct gggtgtcatc
     2521 ctttaccggg gcttgaccgg cgcatttccg ttctcagggc cgtcaaatga cgcgatcatc
     2581 gagcagattc ttcatcacga tcccaaaccg cttcgcatgc acgaccccga tatcgacgcg
     2641 gaactcgagc gcatttgcct ccgctgcctg ttgcgcccga tggccgagag gtatcgcatc
     2701 gcctccgacc taagcgaaga actgctcgcg gcactcgacc ggcccgaacc gcgagaacca
     2761 gccacgagcc cattgatctt gcctcccaag gggtttgcgc cgtacgacaa cgagtacaag
     2821 aagttctttc tcgcgatgct gcccggtccg cggggtgcga atgggctgcc cgagtcgttg
     2881 cgatactgga agcagtgggt cgagtcgact gacagtgatc cagcacaacc cgtgggcctg
     2941 ctctgcggtc catccggcgg aggcaaatct tcgtttgtac gagcgggttt gctcccacac
     3001 ctcgacagaa aacttgtcta ccccatctac gtcgaggcaa ctcgaaccgg aaccgaggcg
     3061 cggctgct
//
LOCUS       DATAQL010000706         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_989201, whole genome shotgun sequence.
ACCESSION   DATAQL010000706 DATAQL010000000
VERSION     DATAQL010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989201"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3309)
                     /locus_tag="VHS97_12305"
     CDS             complement(<1..3309)
                     /locus_tag="VHS97_12305"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX3449031.1"
                     /translation="MIAPLAPLVAAMAAGIIADRWLEPCETKVWVTIALVLGAIAAST
                     TRHTSVFCVAMLAATSAIGGGWHHHRWFDQSVDDLARSVTETPRPAWVRGVVSEARGL
                     RHRRDSYGIGKNAEEKVTTRFVLDITSISDGERWHNASGRALVVVAGDRSEIRAGQAI
                     ETAGQIATIAGRLNPGEFDYRAFLQAQGIRLRMTIDNAASFWLDPRGTDWSFTLWLDR
                     HRSWSRTRLFSWLDPTTAPLAAALLLGWREEIDPEINDAFARTGTTHLLAVSGLQLQA
                     LAISLLLIFRVIGFPRRISYLVVGVTMIGYALLVGPAPSVVRSTVMTSTFCLAAIAQR
                     LSRSANTLSLAALCTLAINPMNVFDVGCQLSFLAIGALVWLVSPACNLVRDIYETIRS
                     RFFGPRSALDFLERQLEPWWRTALRQVGAKVIDGIVASTVVWLAALPLVAFQFHIVSP
                     IGILLNIPLIPITSAALLLGGLSLVLSLAWGALGSSLAWAAAWLLKLTQTIVLWGVAQ
                     PLGHRFVVGPAWDWVLLFYFLLGVAAVATTTSRPRVLTTWTARVWRYGVWCLLAAWFV
                     PGWWLAGIAARDAPLEAEVLAVGHGLAVVLHLPDGQTLLYDCGRLGDPSVGRRIIAPA
                     LWASGVNRIDTVFLSHADQDHYDGLPDLLDRFRITEVRLPPGFAGANNPLAVELIDQL
                     RKRGIPVRPITAPAQWEQAGVQFTVWHPPHGWHPETSDNSRSLVLDVAFAGHHLLLTG
                     DLEQPGLDHVVASPPPDPAPDAFLAPHHGGKSSNPDWLYEWAKPRAVIVSQRPQSART
                     GDALAALEERGIPVLRTWRQGSIRLRWVDDRIAASGFIEPFSDQDRNPGHPRTTRPEW
                     SSITPRLRPATAVADWRRASAVRFIVGGIGLSLGASTCLVLVVIEFGAWVLVAPPRAM
                     NADNEEADDSTKIDQLRAEPIEIRAADGARLVGRLLPVSAAIATGRTALLLHGFAEAS
                     SVLEARRAAALNRHGWNVAVLDSRGYGQSGGAFGTFGGLEAGDIRLWLDLLAERIART
                     GPGTVLRPVLWGRSMGAAIAMRATALDHRLVALVLESPMVDLEASMAIVLERRRLPCA
                     KLLARLITGRASKLA"
     gene            3687..>4270
                     /locus_tag="VHS97_12310"
     CDS             3687..>4270
                     /locus_tag="VHS97_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449032.1"
                     /translation="MNHSYPCGGISRRQWLGASAVAAPIFSAAHAVAAAQEQLASGKV
                     ANHGVDAAPLSKLAAPGLYPGHVVEVRNPGMYRGGVRDAAAIKATLDRGIKELTGATD
                     AVEGWKHFFEPGDVVGIKVVPNGQPHAHSSFEIVLEVIEKLKTAGVKTSDIFVYDRYR
                     GEFMEAGYHKILPAGIRCGGLDPEGNQFTLDFPS"
BASE COUNT          855 a         1333 c         1362 g          720 t
ORIGIN      
        1 agccagcttg ctggcgcggc ctgtgatcag ccgggccaag agcttcgcgc agggcagcct
       61 gcgcctctct aatacgatgg ccatggaggc ctcgaggtcg accatgggcg actcgagtac
      121 cagcgcgacc agccggtgat ccagggcggt ggcccgcatg gcgatggcag cgcccatcga
      181 gcggccccat agcaccggac gtagaactgt gcccggaccg gtgcgcgcga tccgctcggc
      241 gagtaaatcg agccagagac ggatgtcccc tgcctcaagt ccaccgaaag tcccgaaagc
      301 gccgccgctc tggccataac cgcgcgaatc aagcacggcg acgttccagc cgtgcctgtt
      361 caaggccgcc gcgcggcgag cctccaggac ggaagaggct tccgcgaacc cgtggaggag
      421 tagcgcagtc cgtccggtag caatcgccgc tgagaccgga agcaagcgtc cgaccagccg
      481 cgctccatcg gctgcccgaa tctcgatagg ctcggccctt agctggtcga tctttgtcga
      541 gtcgtcggct tcttcattgt cggcattcat tgctcggggg ggcgctacca acacccaagc
      601 cccgaactcg atcacgacca ggaccagaca ggtgcttgcc ccgagcgata ggccgatgcc
      661 gcctactatg aagcgcacag cgctcgctct tcgccagtcg gccacggccg tcgccggcct
      721 aaggcgtggc gttattgacg accattccgg cctggtcgtc cgtgggtgac ctgggtttcg
      781 atcttgatcg ctgaacggct cgataaaccc gctggcggcg attctgtcgt cgacccaccg
      841 caaacggatc gacccctgtc gccacgtacg aaggacgggt atgccccttt cctcgagagc
      901 ggcgagggca tcgcccgtgc gggcggattg ggggcgctgg ctgacgatga ccgcgcgggg
      961 ttttgcccac tcgtagagcc aatccggatt ggacgacttg ccgccgtggt gcggggcgag
     1021 aaaggcgtca ggtgcaggat ccgggggagg actcgccacc acgtggtcga ggcccggctg
     1081 ctcgagatcg ccggtcaaga gcagatggtg ccccgcgaag gcgacatcga gcaccaggct
     1141 gcgcgagttg tcggaagtct ccgggtgcca accgtggggc gggtgccaaa ccgtgaactg
     1201 gacgcccgcc tgttcccact gcgcgggggc cgtgatcggc cgcaccggga tgccgcgttt
     1261 ccgcaattgg tcgataagct cgaccgccaa tggattgttg gctcccgcga aaccgggagg
     1321 gagccgcact tcggtgatcc tgaatcggtc gagcaggtct ggtaggccgt cgtaatggtc
     1381 ctggtcggca tggctcaaaa agacggtgtc gatgcggttg actccgctag cccaaagtgc
     1441 cggagcgatg atcctgcggc ccacggaggg atcgcccagg cgcccgcagt cgtacagcaa
     1501 agtctgtcca tcgggcaggt gaagcacgac tgcaaggcca tgcccgactg ccaggacttc
     1561 ggcctcgagg ggggcgtcgc gcgccgcgat gcccgcgagc caccaaccgg gtacaaacca
     1621 cgccgcaagc aaacaccaga cgccgtagcg ccagacgcgc gcggtccacg ttgtcaacac
     1681 ccgcgggcgc gaggtggtcg tggcgacggc ggccactccc aagaggaagt agaataagag
     1741 cacccagtcc caggccgggc caacgacgaa tcggtgtccc agcggttgtg cgacgcccca
     1801 caaaacgatc gtttgagtca atttcaatag ccatgcagcg gcccaggcga gactgcttcc
     1861 gagcgctccc caggccagtg ataacaccag agatagccct cccagtaaga gcgcagccga
     1921 ggtgatcgga atgagcggaa tgttcaggag gatcccgatc ggcgagacaa tatgaaattg
     1981 gaaagccacc agcggcaagg ccgccagcca gaccacggta gaagccacga tgccgtcgat
     2041 cacttttgcg ccgacctgac gcagcgcggt gcgccaccag ggctcgagct gccgctcgag
     2101 gaaatcgagc gccgagcgtg gaccgaaaaa acggcttcgg atcgtttcat agatgtctcg
     2161 aactaagttg cacgcgggtg acaccagcca caccaaggcg ccgatcgcga ggaaggaaag
     2221 ctggcagccc acatcgaaaa cattcatcgg attgatggcg agcgtgcaca gtgccgcgag
     2281 tgaaagcgtg ttggcggatc gcgacagccg ctgggcgatc gcggccaggc agaaagtcga
     2341 ggtcatgact gtcgagcgca cgacggaagg ggccggccct acgagcaggg cgtagccaat
     2401 catggtcacc cccacgacca gataggaaat ccggcgggga aatccgatca cgcggaaaat
     2461 gagcaagagg gaaatggcga gcgcctgcaa ttgcagacca gagacggcca acagatgagt
     2521 cgtgcctgtg cgagcgaacg cgtcattgat ctcgggatcg atctcttcgc gccagcccag
     2581 cagcaaggct gcggccaggg gagccgttgt cggatcgagc cagctaaaga gccgcgtgcg
     2641 gctccaggaa cggtgacgat ccagccagag cgtgaaactc cagtccgtcc cgcgcggatc
     2701 aagccagaag ctcgccgcgt tgtcgatcgt catccgcagc cggattccct gggcttgcag
     2761 aaaggctcga tagtcgaact cgccgggatt gagccgcccg gcgatcgtgg caatttgacc
     2821 tgccgtctcg atggcctgtc cagcacggat ctccgatcga tcaccggcaa caaccacgag
     2881 tgctcggccc gaggcattat gccagcgctc gccgtcgctg atggatgtga tgtcgagcac
     2941 gaaccgggtt gtcacttttt cttcggcgtt cttgccgatt ccgtagctgt ctctgcggtg
     3001 gcgcaatccg cgtgcctcgc tcaccacgcc gcgcacccat gccggccggg gtgtttcggt
     3061 gacgctcctg gccaggtcgt cgacgctttg gtcgaaccag cgatggtgat gccagccacc
     3121 gccgattgcc gaagttgccg ccagcatcgc cacgcagaag acagacgtat ggcgagtcgt
     3181 cgatgccgcg attgcgccca aaaccagggc aatcgttacc cagactttcg tttcgcaagg
     3241 ctccagccag cgatccgcga tgataccggc cgccatggcc gctaccaaag gcgccagcgg
     3301 ggcgatcaca aaccgaatcg ggtcctcgcg ttggctttga atggccgacc gagacccgac
     3361 gcccgaatcg cggcgctctg gagaattccc gcctcgagcc cacattccgt gggattggat
     3421 cgaggcgggt tggtccacga aacggtcccc agtcaattgt gttgtgtcac cggcgtgtgt
     3481 tcgtaaaaac gtaccatttc gatggcttga ctgggtaggc aatgggtggg tggatttaat
     3541 tctcgccggt gacgacgacc tgccgagaag gttccagttc tcctgactga actgttggcg
     3601 ggtgacagca agagcgatat actcattggt cacctacgta cctggacacg cgtcatcgcg
     3661 cacgataccc cacaacggac caaaagatga accatagcta cccatgtggc ggcataagcc
     3721 gccgtcagtg gctgggcgct tcggcggtcg cggctccgat tttctcagct gcgcacgccg
     3781 ttgccgccgc gcaggaacag ctcgcctctg gtaaagtggc aaatcatggt gtcgatgccg
     3841 ccccgctgtc gaagctggcc gcgcccggtt tgtatcccgg ccacgtcgtc gaggtgcgca
     3901 acccaggaat gtatcgtggc ggcgttcgcg atgccgccgc gatcaaggcg accctggatc
     3961 ggggcatcaa agagctgacc ggcgcgaccg acgccgtgga aggatggaag catttcttcg
     4021 agccgggcga cgtcgttggt atcaaggtcg tccccaacgg ccaacctcat gcccattcgt
     4081 cgtttgagat cgtcttggaa gtgatcgaaa agctcaaaac ggccggcgtc aagaccagcg
     4141 atatctttgt ttacgaccgt tatcgcggcg agttcatgga agccgggtat cacaagattc
     4201 tccccgccgg catccgatgt ggcggactgg accccgaagg caatcagttc accctggatt
     4261 tcccgtcatt
//
LOCUS       DATAQL010000707         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_684552, whole genome shotgun sequence.
ACCESSION   DATAQL010000707 DATAQL010000000
VERSION     DATAQL010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684552"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VHS97_12315"
     CDS             <1..597
                     /locus_tag="VHS97_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449033.1"
                     /translation="WVSSTWRSSMDGFGLRGDQLDSDFSRDVHDQLFQARPGADYGYT
                     DSNWRGVNPMLTPGAEGYLRVTSTTFDNSFNRGPYTLHDPANPDGLGPSSSADGCAFS
                     YSSLLPPALQGNIFIVRYNGTITEAPGGQNASLTYSDLVAVNITTGKVMRVAFGFTNP
                     LAVLADGQSGRLLIADYGAKVVYALQYLGGVCPAARAH"
     gene            complement(730..1887)
                     /locus_tag="VHS97_12320"
     CDS             complement(730..1887)
                     /locus_tag="VHS97_12320"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX3449034.1"
                     /translation="MGLCSNRRDFLRVAGASLLAPLVELPQLRAAGPNPSLPVGIARC
                     NDYNPETVLQQLQSMMDKLGGLDKLVAGKTVAVKVNLTGNVRQQALDLPASRTYHVHP
                     NVVMATATLLDRAGARRIRFLECTYQLGEFEPYLHDAGWDMNALAAMKAAVEYEDTRN
                     LGKGKKYHEVKVPWGGSLFPAYHLNHSYVDCDVYVSLAKLKNHATAGVTMGIKNNFGI
                     TPPALYSHHEPNERATTARMALFHSGKERPADGLPQEVDPKSPRRPTYRVPRHTVDSI
                     GIRPIDLTIIDGIETVSGGEGPWLKLAVQKPGLLLAGRNPVCTDAVGTAVMGYDPMAA
                     PGTGVFPGDNHLAMAAKLGLGTHNPNEIEVVGLSIKEALHPFGWLPRERNG"
     gene            2321..>2823
                     /locus_tag="VHS97_12325"
     CDS             2321..>2823
                     /locus_tag="VHS97_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3449035.1"
                     /translation="MNDDRVAEPECWVDRHGDVLYRYALLRLRSADRAADVVQETFVE
                     ALRARGTFAGKSTERTWLIGILRHKIVDQIRGSARDPASSNGVLLDAVPEPAFDHRGR
                     WRTSPGAWNADPSRTVETHEFWEVVGGCLSKLPKGIAEAFFLRELDGMTAEEVQQMLG
                     ITPANFW"
BASE COUNT          541 a          826 c          885 g          571 t
ORIGIN      
        1 tgggtgagct cgacgtggcg atcgtcaatg gacggattcg gcctccgcgg cgaccagctc
       61 gactccgact tcagccgtga tgtccatgat cagctctttc aggcgcgtcc tggcgccgac
      121 tacggctaca ccgacagcaa ctggcgcggt gtgaatccga tgctcacacc gggggcggaa
      181 ggctatctgc gcgtaacgtc cactacgttc gacaactcct tcaacagggg tccctatacg
      241 ctgcacgatc cggccaatcc ggacgggctc gggccaagca gttcggccga cgggtgcgct
      301 ttctcttact cgagcctcct gcctccagcg cttcaaggca acattttcat cgtgcgttac
      361 aacgggacga tcaccgaggc gccgggaggc caaaatgcct cgctgacgta ctcggacctc
      421 gtcgcggtca atataacgac cggtaaggtg atgcgcgtcg ccttcggatt cactaacccg
      481 ctagctgtcc tggcggacgg tcaatcgggc cgcttgctca ttgccgatta tggtgccaag
      541 gtcgtttacg cgctccagta tctcgggggt gtatgtccgg cggctcgggc tcactaagtg
      601 gacccgccga agtcgtcgcg cagcgtaggc cgggcctttt agcccgacat tttactcgag
      661 ggcacaaaac gtcgggctag aaagcccgac ctacgcgagt agttttgcgg tctcactagg
      721 accctcggct caaccgtttc gctcgcgcgg tagccaaccg aaggggtgca gcgcttcctt
      781 gatcgacaac cccacgactt cgatctcgtt cgggttgtgc gtcccgagtc ccagcttggc
      841 ggccatggcc aaatggttgt cgccggggaa cacaccggtt cccggtgcgg ccatcgggtc
      901 gtatcccatg accgccgtgc caacggcgtc ggtgcaaacc gggtttcggc cagccaacag
      961 caatcctggc ttctgcacgg cgagcttcag ccacggcccc tcgccgccag agaccgtctc
     1021 gatcccatcg atgattgtaa ggtcgattgg ccggatgccg atcgagtcga ccgtatgtcg
     1081 cgggactcgg tacgtcggcc gacggggtga cttggggtcg acctcctgcg gaaggccgtc
     1141 cgccggcctc tctttgccac tgtggaaaag ggccatgcgg gccgtcgtgg ctcgttcgtt
     1201 gggctcgtgg tgtgagtaaa gcgccggggg ggtgatcccg aaattgttct tgattcccat
     1261 cgtcacgccg gccgttgcat ggttcttgag cttggccagc gacacgtaga catcgcagtc
     1321 gacgtaggaa tggttcagat ggtaggcagg gaagaggctg ccgccccatg gcaccttgac
     1381 ctcatggtac ttctttccct tgcccaggtt gcgtgtgtct tcatattcga cggcggcctt
     1441 catcgcggca agagcgttca tgtcccaacc ggcatcgtgc aggtacggct caaattcgcc
     1501 aagctggtag gtgcactcca ggaagcgaat gcggcgggcg ccagcccggt cgagcagggt
     1561 cgcggtggcc atgacaacgt tcggatgaac atgataggtt cggctagcgg gcaggtcgag
     1621 cgcctgctgg cggacgttac ccgtaagatt gaccttgact gcaacagtct tgccggcaac
     1681 cagtttgtcg aggccgccca gcttgtccat cattgactgg agctgttgga gcacggtctc
     1741 agggttgtag tcgttgcaac gggcgatgcc cacgggcaat gacggatttg gcccggcggc
     1801 acgaagttgc ggtagctcta ccagcggggc cagaagggac gctcctgcga cccggaggaa
     1861 atctcgacga ttgctacaca atcccatggt tgttctccgt gcttcgggag gggatacaat
     1921 aggatgcggt gtcaggagcc caggacaccc cagggcgacc agccctcgat cctcgacttc
     1981 atccagtttg gctaagcgct caatacccgc tttgagaaag gttttcgcga gcctacgcca
     2041 actgtaagga gatgtcaaga aactttcttg caaagacatc agtgattgta acgaatctgg
     2101 tcatagccgc ttattgaagc ggcccagcca gccggttgaa gtggctagac aaccggcaaa
     2161 cgatgcggtc gaggagaagg cggttcgtca attgaaggag accgatcaaa cgcgacgcgt
     2221 atcgtgctca tcgcggataa tcgatgtcag gaatttgggc gatcgagcga ctcatcgtta
     2281 tgagaagtga atccggagaa cgttcgacgg accgcggtcg atgaatgacg atcgggttgc
     2341 cgagccagaa tgctgggtcg accggcacgg cgacgtgctc tatcgctatg cgcttctgcg
     2401 actccgttct gccgaccgag ccgcggacgt cgtccaggaa acgttcgtgg aagcgcttcg
     2461 tgccagggga acgttcgctg ggaaatcgac cgagcggacg tggttgatcg gtatcctgcg
     2521 gcacaagatc gtcgaccaga ttcgcgggtc ggcgcgcgac ccagccagtt ccaacggtgt
     2581 actgctcgat gcagtcccag agccggcctt tgaccaccgt ggccggtggc ggaccagtcc
     2641 gggggcgtgg aacgccgatc ccagccgtac cgtcgagacg cacgagtttt gggaagtcgt
     2701 cggcggatgt ctatcgaaat tgccaaaggg aatcgccgag gccttcttct tgcgcgagct
     2761 cgatgggatg accgcggaag aggttcaaca aatgctcgga atcacgcccg ccaatttctg
     2821 gaa
//
LOCUS       DATAQL010000708         5468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4500061, whole genome shotgun sequence.
ACCESSION   DATAQL010000708 DATAQL010000000
VERSION     DATAQL010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4500061"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            666..1448
                     /locus_tag="VHS97_12330"
     CDS             666..1448
                     /locus_tag="VHS97_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449036.1"
                     /translation="MLLAPAGGCRGDFARSIDRRPFQSAVAVKMMVPGWAHLSWGQRQ
                     RGWILVGSFAAAILVGLWAWGTWVGWGLFAFAFITHITSATDVLRQASFPSYPSRLAL
                     MIFAGALAFILYLPALFFLSVIARPGFEADGAGSGFLVNCWAYQDGSEPREGQWVWMR
                     FPSRETPHAGRVVATAGQKVEWNGRQWRVNGHECLAHSILRLDAWPQPCTFKVPSHQI
                     LVEPDDDAASAPNLGPLVLVSPDRIIGRAWAHFYPVWDRHLL"
     gene            1854..2276
                     /locus_tag="VHS97_12335"
     CDS             1854..2276
                     /locus_tag="VHS97_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX3449037.1"
                     /translation="MCRAVAGDQDRDNLVVYRGTNSFVVLNRYPYNNGHLLVSPCVHR
                     GTLGELKGPELSEPIETVQRMMGILDRMLQPQGYNVGLNQGKAAGAGLPGHLHWHIVP
                     RWDGDTNFMPILGQAKVLVESLDAFYERLLAELHRGLA"
     gene            2386..3489
                     /locus_tag="VHS97_12340"
     CDS             2386..3489
                     /locus_tag="VHS97_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX3449038.1"
                     /translation="MLLVLIRAIFVLVVAAYGVSTAKLVTEHQIANPYIVFIAVMLAA
                     VGVVVADLLTPRKRIQAISAVFLGLTVGFLLSNLLKDALQPTMQQYIDPRVHQLVGLL
                     MLIFICYVCISTLLQTKDDFRFIIPYVEFSKEVKGARPLVLDTSVVIDGRIADVAETR
                     VIDQPMIIPRFVLQELQSIADSSDKLRRNRGRRGLDILNRLQKSPGIEVRIHDAEIPE
                     LAGIREVDQRLVVLAKHLGGKVVTNDYNLNKIARLQGVEVINLNDLANAMKPIVLPGE
                     ALTVKLLKRGEEQGQGIGYLDDGTMVVAEQGAYHLGETLRLSVTSVLQTSAGRMIFGR
                     IDPVQPPKTTTVSAGPASHQDSQSPAPGRHQVS"
     gene            complement(3521..4777)
                     /locus_tag="VHS97_12345"
     CDS             complement(3521..4777)
                     /locus_tag="VHS97_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3449039.1"
                     /translation="MIEHKTDPGSEQHETTERYDLEVAPRVRNLPPYLFGRINELKYR
                     KRRDGIDVIDLGMGNPTDPPEEWVIDKLCEAARDSRNHRYSAATGIYNLRKEVANRYE
                     SRFGVSLDPDQEVVATIGSKEGFSHMCLALLGPGDTALVPAPSFPIHIHAVALASANV
                     ISLDVRDGSTFLKNIARTCESLYPRPKILVINYPHNPSSTVVDQPFFEEIVALAKKYR
                     FFVIHDFAYGDIGFDGYQPPSFLSVKGAKSVGCEFTTMSKGYNMAGWRVGFAAGNRDM
                     LGALKAIKGYYDYGIFQAVQVASIVALRKGEAGRLAQVAEYQSRRDAFVRGLKRLGWD
                     LEPPQAGMFVWTAIPEPWRSRMGSIDFAMKLLEEANVAVSPGRGFGETGEGFLRLALV
                     ENDQRLRQAVRQIGRCLRPEQGIAVP"
     gene            complement(5246..>5468)
                     /locus_tag="VHS97_12350"
     CDS             complement(5246..>5468)
                     /locus_tag="VHS97_12350"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3449040.1"
                     /translation="GLLVATVKEGSAAAEEGIQEGDVITKVIKDRKIQPVTSVKDFQD
                     LVSKNDEVSFYVQRGKLGRYVTLSKQHAK"
BASE COUNT         1090 a         1587 c         1576 g         1215 t
ORIGIN      
        1 actcaggcac cgtcgaaagg gtggaactcc ctttgttcgg gctacgaact acacaccaag
       61 atcaatcagc cggatttggt tcaacccgtc gttccaggac gggctgaagg gcatgccgag
      121 gcgagctacg gaggcacacc aaaaccgatc tgagtacaat tctaacgcgt tttggtgtgg
      181 aacatgggaa gagtccaggt agagctaatg aacgcaagcg taatatttta gcacgaccac
      241 ctattcggtc aaggcccaat accatccctg caccacacct tgatcaactt attatttact
      301 atacgcacaa acgcattact tgcgtcgtat ggggtcgtag attccctcga acgaccaagt
      361 gcttgtaatc gggtcttacg atgttgctgc atgacatgtg cggtgatgaa cggctgaaag
      421 acgattttcc gagagttctc acttaacatt gataattcgg ctgcgaatac gtgacgacgg
      481 gacatttcgt ccctgattga cgctaatttg gaattagcca cttgctggcg tggctacaat
      541 tgagggtgta cgtggcgagt ccaccgtgtt gagctcgatc atcgagtttc tgacccgctt
      601 tgcgttgctg ccaagaaaaa caacgaagcg cgttcccttc ctgatttcgt agcaaggagc
      661 cagagatgtt acttgccccg gctggaggct gtcgcggcga tttcgcgcga tcgatcgatc
      721 gccgtccgtt ccagtctgcc gtcgcggtca agatgatggt tcctggatgg gcgcatctgt
      781 cttggggcca acgtcaacgc ggctggatct tggtgggttc gttcgcggcg gcgatcctcg
      841 ttggtctctg ggcttggggc acttgggtgg ggtgggggct ttttgcgttt gccttcatca
      901 cccacatcac ttcggcgacc gatgtgcttc ggcaagcgtc gtttccgagc tatcccagcc
      961 gcttggcact catgatcttt gcgggtgcac tggcattcat tctctatttg cccgcactgt
     1021 tctttctgtc ggtcatcgcc aggcccggtt tcgaggccga tggcgcgggc agtggattcc
     1081 tggttaactg ctgggcatat caagacgggt cggagccgcg ggaaggccag tgggtctgga
     1141 tgcgatttcc ttcgcgcgag acgccgcatg ccggccgggt cgtcgcgacc gctggtcaga
     1201 aagtcgaatg gaacgggcgg caatggcgag tcaacggcca cgaatgcctc gcccactcca
     1261 tactccgact cgacgcatgg ccgcagccct gcacgttcaa agtgccgtca caccagatct
     1321 tggtcgagcc cgatgatgac gccgcgtctg cccccaacct cggaccgctc gttcttgtct
     1381 cgcccgatcg aatcatcggg cgggcctggg ctcatttcta ccccgtctgg gacaggcatc
     1441 tgctttaaag ctttggtatg taacggattg agcgtcgaca agttctcccc cgcctggtgt
     1501 tctccgccaa cggatcacgt cgtcaatctc gcaaatcgac ctgaatgatg cctataataa
     1561 acgcgatttg ctgtcgcgat acgtagtgtc gtcgatcctg acgtgaaaaa aacatgtgca
     1621 atcgcgacaa gaagcgacca cgatgatatc atcgtgggaa gtggagttcc gtcgtgctcg
     1681 ttccgtcatc ccaccacggc ttacgcccgg ccgaggtcgg atcgcgccag ttttgggagt
     1741 ccactggaac catttcggag tgacgggtcg tgctcgatcg gatatgggct ccgtggcgcg
     1801 cacactacat ccattcggca gccaccgagc cccgacgggc agacgagtgt ttcttgtgtc
     1861 gggcggttgc aggcgatcaa gatcgggaca acttggtcgt ttatcgtggc acgaattcgt
     1921 tcgttgtctt gaatcggtat ccgtacaaca atggacattt gctggtttcc ccgtgtgtgc
     1981 atcgaggtac gcttggagag ctcaaggggc ccgaattgtc cgagccgatc gagacggttc
     2041 aaaggatgat gggcattctc gaccgtatgc tgcagccgca gggttacaac gttgggctca
     2101 accagggcaa ggctgcgggt gccggcctcc ccggccattt gcactggcat atcgttccgc
     2161 gctgggatgg cgacaccaac ttcatgccaa ttctgggaca agcgaaggtg ttagtcgaaa
     2221 gtttagacgc attttacgaa cgactcttag cggaattgca tcgcggccta gcataaagcc
     2281 actgcagccg gaatctgcgg ccggctgacg gtggacaaac ctttctcgta tctattttcg
     2341 ccgcgaacag tgggattgcc gtcgagtcag gaagaggttt tcacaatgct actcgtattg
     2401 atccgggcca ttttcgtgct tgtcgtcgcc gcctacggcg tcagcactgc gaagcttgtc
     2461 acagaacatc aaatcgccaa tccgtacatc gttttcatcg cagtgatgct cgcggccgtt
     2521 ggcgtcgtgg tcgccgatct tttgactccc cggaagcgaa ttcaagcaat ttccgccgtt
     2581 tttcttgggc tgactgtcgg gtttcttctt tccaatctgc tgaaagacgc gctgcagccg
     2641 acgatgcagc aatacatcga tcccagggtc caccaattgg tcgggctcct catgttgata
     2701 ttcatctgct acgtttgcat ctcgacgctt ctgcagacca aggacgattt tcggttcatt
     2761 atcccctatg tcgaattctc gaaggaagtc aaaggggcac gtcctctggt gctcgacacc
     2821 tcggtcgtga tcgacggccg gatcgccgac gtcgcggaga ctcgcgtcat cgatcagccg
     2881 atgattatcc ctcggtttgt tttgcaagag ctgcagagta tcgccgacag ctcggacaaa
     2941 cttcgccgca accgcggacg ccgggggctc gacatcttga atcgcctgca gaagtcaccc
     3001 ggcatcgaag tccgcatcca tgacgccgag atccccgagc tggccgggat ccgcgaggtc
     3061 gatcagcggc tcgtcgttct ggccaagcat ttggggggaa aggtcgtcac caacgactat
     3121 aacctcaaca aaatcgctcg cctccaggga gtcgaggtga tcaacttgaa cgacctggcc
     3181 aacgcaatga agccgatcgt acttcccggc gaggccctga cggtcaagct cttgaagcgc
     3241 ggggaagagc aagggcaagg catcggttac ttggacgacg gcaccatggt cgtcgccgaa
     3301 cagggagctt atcatctggg cgagacccta cgcttgtcgg tgacgagcgt cctccagacc
     3361 agcgcgggcc ggatgatttt tggccggatc gatcccgttc agcccccgaa gacaacaacc
     3421 gttagcgcgg gtcccgcgtc tcaccaggac agccagagcc cggctcccgg ccggcaccag
     3481 gtttcgtgag cggccggctg ggtcgggtcc ataccctttt tcatggaacg gctatgcctt
     3541 gctccggccg caggcagcgc ccgatttggc ggacggcttg ccgtagccgt tgatcgtttt
     3601 ccaccaaggc gagtcggaga aaaccttcgc cggtctcgcc aaagccgcgt ccgggactga
     3661 cggccacatt ggcttcttcc agtaatttca tggcaaaatc gattgagccc atccgcgacc
     3721 gccacggctc ggggatcgcg gtccagacga acattccggc ctggggaggc tcgagatccc
     3781 agcccaggcg cttgagaccg cgcacaaacg cgtcgcgtcg actctgatac tcggcgacct
     3841 gcgccagccg gccggcctcg cctttgcgca gcgccacgat cgacgcgacc tgaaccgcct
     3901 ggaagattcc gtagtcgtaa tagcccttga tggccttgag cgcacccagc atgtcgcgat
     3961 tgcctgccgc gaagcccacc cgccagccgg ccatattgta gcccttcgac atcgtcgtga
     4021 actcgcagcc gacgctcttg gctcccttga ccgataaaaa gctcggcggc tgataaccgt
     4081 cgaaaccgat gtcgccatag gcgaagtcgt ggatgacgaa gaatcggtac tttttggcaa
     4141 gcgcgacgat ctcttcaaaa aagggctgat cgactacggt ggagctcgga ttgtggggat
     4201 agttgataac caggatcttc ggtcgggggt acagactctc gcaggtgcga gcgatgttct
     4261 tcaggaaagt cgacccgtcg cggacgtcga gggaaatgac attggctgaa gcgagggcca
     4321 cggcgtggat gtggatcgga aagctaggcg ccggcacgag tgccgtatcg cctggtccca
     4381 gcagagccaa gcacatgtgg ctgaagcctt ccttggaacc gatggtggcg acgacctctt
     4441 ggtccggatc gaggctcaca ccaaaacgcg attcgtagcg gtttgcaacc tccttgcgta
     4501 ggttgtagat gccggtcgcg gcactatagc ggtggttgcg cgagtcacga gcggcctcgc
     4561 agagcttgtc gatgacccac tcttcgggcg ggtcggtggg attgcccatc cccaggtcaa
     4621 tcacgtcgat accgtcgcgc cttttgcggt acttgagctc attgatgcga ccgaaaagat
     4681 acggtggcag gttgcgcacg cggggagcca cctcgaggtc gtaccgctcg gtggtctcat
     4741 gctgctcgga acctggatcg gtcttgtgtt caatcatcat ggctctcgtt cgggaccgtc
     4801 agtcgatccg tgaggatcga atggagctcc gcctccctta ccgtggatgg tcaccacaac
     4861 gctccctctt ccgcaggccc gcgcccgcgc atcccaacga ttcaaatctc tctcacattt
     4921 actctagcat ccgaccgttg tcgatgcacc gcgcgtttga gaggaagact gtagcggttg
     4981 tgacgccgcc caaagaaggc cgccgggaat tgatgaaata tctgcgacca agcgagtcga
     5041 tactcgaaat accaccagga cgacccctcg acttggatgg aaatcacctc gatcgacccc
     5101 gcggactcgg gtcaatctgg attatcataa atgaagaggg gcggcccgcg tttatcggac
     5161 cgcccctctt ttgtaacgaa ctcgttgaca ctcgctgcgg gcttcttcac agttcttcag
     5221 agagaagcgt caactcgcat gggcatcact tggcgtgctg cttggagagg gtgacgtagc
     5281 gccccaattt gccgcgctgc acatagaacg aaacctcatc gttcttcgag accaggtcct
     5341 ggaagtcctt gacgctcgtg acgggctgga tcttgcgatc cttgataacc ttggtgatca
     5401 catcgccttc ctggatccct tcttcggccg cagcgcttcc ctctttcacg gtggcgacca
     5461 acaagcct
//
LOCUS       DATAQL010000709         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4042579, whole genome shotgun sequence.
ACCESSION   DATAQL010000709 DATAQL010000000
VERSION     DATAQL010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4042579"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2898)
                     /locus_tag="VHS97_12355"
     CDS             complement(<1..2898)
                     /locus_tag="VHS97_12355"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3449041.1"
                     /translation="MDEQCPETEPDSKLQPLRLPRHLDDTIRDDEQPDRLLEMLDQWE
                     ERFFLAGDQSPQSLGIDDPVLLAAFLERIEERKRLYESVGLTTRTGPIGAYGEEKAPG
                     GSENSSPLTVPSNGSDQTVPAAPAQADPSAIGRYRLIRLLGKGAFGRVYLARDSVLDR
                     LVAIKVPVTKRAAEFIDVKTYRDEARTLSQLSHPNIVPVYDVGATSECPFFVVSKYID
                     GGDLSTRLARGRPAFAESAELVAVICDALHYTHMQDLFHRDIKPANILIDSAAVPYLA
                     DFGLALKDENFGTGARFVGTVAYSSPEQARGEGHLVDGRSDIFSLGIVFYEMLTCRRP
                     FRGDSHQKILEQIIGSEPRPPRQIDDTIPRELERICLKALSKRAAERYTTASDMAEDL
                     RHFLKTANDDSVSQALSSPPVPPPLVAPAEVVSSTTWARSDLSAGPLRIIPKGLGSFD
                     EHDADFFLALLPGPRDRDGLPDGLRFWKTKIEATDPDRTFRVGMIYGPSGSGKSSLVK
                     AGLLPLLGKHVTPVYLEATTGETVGRLLRGIKKAVPGLPEHAGLVDSFLALRRGRYLR
                     PGRKMLVVLDQFEQWLFARGHEQGDELVAALRQCDGEKIQVLCLVRDDFWMAATRFSK
                     DLEIDLVPDRNIAAVDLFEPKHARKVLAAFGRAYEALPAGAGDLTRDQHAFLDQAIAA
                     LARDGQVVPVRLALFAEMVKGKPWTPATLREVGGMEGVGVKFLEETFSSRRSSPRYRY
                     HFNAVLLVLKSLLPDTTADIKGRMRSIEELRDVSGYTLRPGDFSDLVRILDNDLRLIT
                     PVDLESSLENVVPADLAAGGRYYQLTHDYLVRSLRDWLNRKQRETRRGRAELLLAERA
                     ALWNTKPERLHLPSAREWLWIRLWTRRADWTAAQKRMMTRASRVHGVRLVGFIAAIAL
                     FAGLGAAYSVYVQTNQLLISLSSEVERIPQVLRQLKRYPRWTYDGRLRELAK"
     gene            complement(3327..3758)
                     /locus_tag="VHS97_12360"
     CDS             complement(3327..3758)
                     /locus_tag="VHS97_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449042.1"
                     /translation="MSATTTNQAHIAYELINDTDPGVVVIEFRSHEVASFLHSRELGE
                     QLQSLIGTELPQNFVIDFANVRSLGNTAFGEIVSFARKVKHLCVCNVQDQLRLGASLI
                     GLDDFGDLGASRRDAIDLAKRMAVQDQKETADYPALGDESR"
BASE COUNT          764 a         1234 c         1163 g          739 t
ORIGIN      
        1 cttcgccaac tcacgcaggc gtccgtcata agtccagcgc ggataacgct tgagctggcg
       61 tagaacctgc ggaattcgtt cgacctcgct cgaaagcgaa atcaacagct gattggtctg
      121 gacgtacacg ctgtaagcgg cgcccagacc ggcaaacagg gcaatcgcgg cgataaagcc
      181 aacaagcctc actccgtgca cccggctagc gcgcgtcatc atgcgctttt gcgcggcggt
      241 ccagtccgcc cgtcgcgtcc agagtcggat ccagagccac tcgcgggctg atggcaggtg
      301 aagacgctcg ggcttggtat tccacaaggc cgcgcgttcc gcgagcaaga gctccgctcg
      361 tccccgccgg gtttcgcgct gcttgcggtt aagccagtcg cgtagagaac gaaccagata
      421 atcatgcgtc aactgatagt accgcccccc tgccgcgaga tctgccggca cgacgttttc
      481 gagtgagctc tccaggtcga cgggagtgat cagccgcaag tcgttgtcga gaatccggac
      541 cagatcgctg aagtcgccgg gcctcagggt gtaacccgac acatcgcgca gctcttcgat
      601 cgaccgcatc cggcccttga tgtcggcggt cgtgtcaggc aacagcgact tcaagaccaa
      661 cagcacggca ttgaagtgat agcgatacct gggcgaagac cgccgcgagc tgaaagtctc
      721 ttcgagaaac ttgactccga ccccctccat tcctccgact tcgcggagcg tggcgggtgt
      781 ccagggcttg cccttgacca tctcggcaaa cagagccagc cgcaccggca caacctggcc
      841 gtcgcgagcc agggcggcga tggcctggtc gagaaacgcg tgctgatccc tggtgagatc
      901 gccggctccg gccggaagtg cctcgtatgc gcgtccgaac gcggccagaa ccttgcgcgc
      961 atgctttggt tcaaacagat ccacggcggc gatgttccgg tccgggacca ggtcgatttc
     1021 gaggtccttt gagaaccgag tcgccgccat ccagaagtcg tcgcgcacca ggcagagcac
     1081 ctggattttt tcgccgtcgc actgcctcag cgcggccacg agctcgtcgc cttgctcgtg
     1141 gccacgcgcg aaaagccact gctcgaactg gtcgagcacg acgagcatct tgcggcccgg
     1201 gcggagataa cgcccgcgcc gtagagctag aaacgaatcg accagtccgg cgtgctcggg
     1261 cagcccagga actgccttct tgatgcccct caagagccga cccaccgtct cacccgtcgt
     1321 tgcctcgaga tagaccggcg tgacatgctt gcccaagagc ggcaagagtc ccgccttgac
     1381 caacgacgat ttaccgctcc ccgacggacc atagatcatt cccacgcgaa aggtcctgtc
     1441 agggtctgtc gcctcgatct tcgtcttcca gaaccgcagc ccgtccggca gtccgtctcg
     1501 gtcgcgcggg ccgggcaaca gcgcgaggaa aaagtcggcg tcgtgctcgt cgaaactgcc
     1561 cagtcccttg gggatgatcc gtagcggccc cgcgcttaaa tccgaccgcg cccaagtggt
     1621 gctcgatacc acttccgcag gcgctaccaa aggcggcggc acgggaggcg acgacaacgc
     1681 ctgcgacaca ctgtcgtcgt tcgccgtctt caagaaatgc cgcaagtctt ccgccatgtc
     1741 gctggcggtc gtgtagcgct ccgctgcccg tttcgagagc gccttcaggc aaatccgctc
     1801 gagctccctg ggaatcgtgt cgtcgatctg acgaggaggc cggggctccg atccgatgat
     1861 ctgttccagg atcttctggt gcgagtcgcc ccgaaacggg cgccgacacg tcagcatttc
     1921 gtagaagaca attcccaggc tgaagatgtc tgaccggccg tcgaccaagt gaccctcgcc
     1981 ccgtgcctgc tcggggctcg agtaggctac cgtgccgacg aagcgggcgc ctgtcccaaa
     2041 gttttcatcc ttcagagcca gcccaaaatc ggccaggtac ggaacggccg cagaatcgat
     2101 caggatgttc gcgggtttga tatcacgatg aaacaggtcc tgcatgtggg tgtaatgaag
     2161 cgcatcacag atgacggcga ccagctcagc cgattcggca aacgcgggcc ggccgcgcgc
     2221 cagccgcgtg gataggtcgc cgccgtcaat gtatttcgac acaacgaaaa acgggcactc
     2281 gctcgttgct cccacgtcgt aaaccggcac gatgttggga tgcgaaagct gggagagagt
     2341 ccgtgcctcg tcgcggtacg tcttgacgtc gataaactcg gccgcccgct tggtaacggg
     2401 cactttgatg gccacgaggc ggtccagcac gctgtcgcgc gccaggtaca ccctgccgaa
     2461 cgctcccttg ccgaggagcc ggatcaatcg ataacgacca attgccgacg gatcggcctg
     2521 cgcgggcgcg gccgggaccg tttgatcgct accgttggac ggcaccgtca ggggcgagga
     2581 attctccgag ccccccggcg ctttctcctc cccgtaggcc ccgatgggtc cggttcgggt
     2641 cgtcaggcct actgattcgt acaatcgctt tcgttcttcg atccgttcca aaaacgccgc
     2701 cagcaaaacg ggatcatcga tgcccagcga ctgtggagac tggtccccgg caaggaaaaa
     2761 ccgctcctcc cattgatcga gcatctcgag gagccggtcg ggctgttcgt cgtctctgat
     2821 ggtgtcatcg agatgtcgtg gaaggcggag cggttggagc ttggagtcgg gctctgtctc
     2881 cgggcattgt tcgtccatgc accgctccgc cttgtctttc gggcagctca aaacggatgg
     2941 tcagagccgt ccagcggccg cgccagcccg tgcgatcgcg gcccagcacg tcctgctaga
     3001 ggcagcatgg ttaccgcaat cgaggccaac gctcacaatc tggaggcccg acgagctgtc
     3061 tgatcgacag gtcgcggccg actttcaacg taccgggcgc atcgcggcgt cgagcaaggc
     3121 cgtgcgaaag ctgagcaacc tcgtcacgcc cacatggtac tggcccgaaa cgtatcgctc
     3181 aacattatgg gtttttttct cgaaccctgt cgcggatcga ggcagccaca gccgccgcgc
     3241 tcaagcgggc catcgaaacg ggtattctgc gatcgcgcga cgattcaccg gcctgaatgg
     3301 ccaggctcag ccgcatcgaa gagctttcaa cgagattcgt cccccaacgc cgggtagtcc
     3361 gcggtctctt tctgatcctg cacggccatt ctcttggcaa ggtcgatcgc gtcacgtcga
     3421 ctcgccccga gatcgccgaa atcatccaag cctatcaagg aagcgccgag tctcagttgg
     3481 tcctgcacgt tgcaaacgca cagatgcttc actttgcggg caaaagatac aatttcgccg
     3541 aaagccgtgt tgccgagcga tctgacattc gcgaagtcga tgacgaaatt ctggggaagt
     3601 tccgtcccga ttagcgattg aagctgttct cccagctccc gtgagtgcaa gaaactggcg
     3661 acctcgtggc tgcgaaactc gattaccacg accccgggat ccgtgtcgtt gatcagttca
     3721 taagcaatgt gagcttgatt ggttgttgtt gctgacataa gttcacacct ccgtctttga
     3781 gtacgctgga aattcacggg gcgatcgggt aatgagcgtg caaagaatgc gccgtttttt
     3841 ccgcttgcgg gtggacgcaa ctcatcggtg ggctaaagcg tggagtgtgc atgggcatgc
//
LOCUS       DATAQL010000710         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1905371, whole genome shotgun sequence.
ACCESSION   DATAQL010000710 DATAQL010000000
VERSION     DATAQL010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905371"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..2702
                     /locus_tag="VHS97_12365"
     CDS             318..2702
                     /locus_tag="VHS97_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3449043.1"
                     /translation="MGSPLVEHVASLLSAAVSSGSPVWGALPEVPGGLVWIVGAVLAV
                     IAACELAGLRYIANNRAGIVEKLWSMRGSVPEGRIIALDGEAGFQAEVLRGGLHFGMW
                     RWQYRIHKAPLVSVTKGKIGYVYARDGEALSPSQTLAQIVPCNNFQDARRFLGGEPTS
                     PEHEPVLGQRGRQRAILREGVYAINIALFTVMTEDTVYRLEAGGAKELKALVNWQNEL
                     SQNEGFNPVIIGGPIEASDPLNPDKKTVVDSIGIVTVHDGPSLPPGEIIAPAVGVDRN
                     DKDFHNNFQNPEAFLRAGGRRGLQYVTLTDGTYFINRWFASIESIPKTIVPIGYVGVV
                     VSYYGRSGQDLSGQGFRHGERVAEGERGVQEKPFGPGKYAFNTYAGNIVLVPTTNFVL
                     HWVTGRTEAHRYDESLRSIDLVTKDAYEPTLPLSVVVHIDYQKAPNVIQRFGDVKKLI
                     TQTLDPMLSAYFRDVAHKRTMLELLQDRDSIQNESSNELGRKFHNFDIECVDVLIGKP
                     DTQQAGGKIETLLEQLRMRQLSLEQVETYSKQVTAAQNLRVLNEAQAQAQMQTQLSNS
                     LVQIKIAENEAEAQLARARKQAEQVVVTAEAESQQRILAGRGEGARILQEGFSEASVL
                     LRKISSFSDPRLYALTIAAQNLAKCSQPLVPERVFISAGAGAGGAAGDGQAGTGMGAI
                     ASNGVFGLLLNMLVAEKSGFNLVDQPAGSDLHDFTEKIAKQAMEAIQQAVAANAGTLP
                     AGRQGNGNGVGSTPVPSLATPTVAPVTTRETADSKGKRKDGVRSGGAGPDSGAV"
     gene            3049..3291
                     /gene="rpsR"
                     /locus_tag="VHS97_12370"
     CDS             3049..3291
                     /gene="rpsR"
                     /locus_tag="VHS97_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX3449044.1"
                     /translation="MPRKKFGRNRKNRCRFCTRDGCPRPAYVDYKDIGLLKKLCTNQN
                     KMFSRKRSGSCAAFQRAASSAVKRARFMGLMPYVGE"
     gene            complement(3606..>3858)
                     /locus_tag="VHS97_12375"
     CDS             complement(3606..>3858)
                     /locus_tag="VHS97_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449045.1"
                     /translation="FLMQWQQGTYRDAFLDYVRDAYRGRIKRGPGRSLQERLSQPYTV
                     IDSQLRAFLRNGEDQQRQGKAAVAQPLPSGGTIRTVPRQ"
BASE COUNT          777 a         1154 c         1198 g          729 t
ORIGIN      
        1 ctgcgccgcg agcggcgagc gccaaggtca ggcggtcgtt atcgaccgag cggaatttga
       61 cttcgacctc aaaacccatg caatcgaggg tagaacagcg agggcgttgt cgtcaacggc
      121 ctcgccggcc cgctcagttt ctcccagtgg atttcttgca atattccggg cccttagcga
      181 atagactcgc actccggagg gccgaaccaa cctctggaac ccggatgacc gggtaaattg
      241 cgagggagct gagactcatc tcgggttgtt ccgctttggg cagctttttc gcgctggttc
      301 tcgagaggag ttccaccgtg ggctcgccac ttgttgagca cgtggcatcg cttttgtcgg
      361 cagcagtttc gtccggctcg cccgtttggg gggcattgcc ggaggtaccg ggtggtttgg
      421 tatggatagt gggggcggtt ctcgcggtga ttgcagcctg cgagctggcg ggcttgcgct
      481 acatcgccaa caaccgcgcg gggatcgtcg agaagctctg gtcgatgagg gggtcggtcc
      541 ctgaggggcg gatcatcgca ctcgacggcg aggcggggtt tcaggccgag gtcttgcgag
      601 gcgggctcca tttcgggatg tggcgctggc aatatcgcat ccacaaggcg cccctggtat
      661 cggtaaccaa aggtaaaatc ggatatgttt atgctcgcga cggcgaggcg ctctcgccca
      721 gccagacgct ggcgcagatt gtgccgtgca ataacttcca ggatgcccgt cgcttcttgg
      781 gcggggagcc gacgagcccc gagcacgagc cggtcttggg tcagcgcggc cgccagcgcg
      841 ccatcctgcg cgaaggcgtt tacgcgatca acatcgcgct tttcacggtc atgaccgaag
      901 atacggtcta ccgactggag gccggcggcg ccaaggagct taaggctctg gtcaactggc
      961 agaacgagct ctcgcagaac gagggattca atcccgtgat catcggcggt ccgattgagg
     1021 cctccgaccc gctgaatcct gacaagaaga cagtggtcga cagcatcgga attgtgactg
     1081 tccacgatgg tccctcgctt ccacccggcg agatcatcgc gccggcggtc ggcgtagacc
     1141 gcaacgacaa agactttcac aacaacttcc agaaccccga ggcgttcctc cgggccggcg
     1201 gccgccgggg cttgcagtac gttaccttga ccgacggcac ctatttcatc aaccgttggt
     1261 tcgccagcat cgagtcgatc cccaagacga tcgtcccgat cggctatgtt ggcgtggtgg
     1321 tcagctatta cgggcggagc ggacaagacc tttcaggcca gggtttccgc cacggcgagc
     1381 gcgtggccga aggtgagcgt ggcgtccagg aaaaaccatt cgggccgggc aagtatgcct
     1441 tcaacaccta tgccggcaac atcgtcctgg tgccgacgac caacttcgtc ttgcactggg
     1501 tgaccggacg gaccgaggcg catcgctacg acgagagcct ccgttcgatc gacctggtca
     1561 ccaaagacgc ctacgagccg accttgccgc tgtcggtcgt cgtgcatatc gactatcaaa
     1621 aggctcccaa tgtcatccag cggttcggcg acgtcaaaaa gctgatcacg cagaccctcg
     1681 atcccatgtt gagcgcgtat ttccgcgacg tggcacacaa gcggaccatg cttgaattac
     1741 ttcaagaccg cgactcgatc cagaacgagt ctagcaacga gctgggcagg aagtttcaca
     1801 atttcgacat cgagtgcgtc gacgtcttga tcggcaagcc cgacacgcag caggccggcg
     1861 gcaagatcga gaccctgctc gaacagttgc ggatgcggca gctctctctc gagcaggtcg
     1921 agacctactc taagcaggtc accgcggccc aaaacctgcg cgtcttgaac gaggcccagg
     1981 cccaggcaca gatgcaaacg caactgtcga actcgttggt gcagatcaag atcgccgaaa
     2041 acgaggccga ggcccaactg gcgcgggcgc gtaagcaggc agagcaggtg gtcgtgacgg
     2101 ccgaagcaga aagccagcag cgcattctgg ccggtcgagg cgagggtgcc cggatccttc
     2161 aggaagggtt ctctgaagcg tcggtcttgc tgcgcaagat cagctcattc tcggacccgc
     2221 gcctgtatgc cctgacgatt gccgctcaaa acctggccaa gtgcagccag cctctggtac
     2281 cggagcgcgt cttcatatcg gctggcgccg gcgctggcgg tgcggcggga gacggtcagg
     2341 cgggtactgg catgggtgcg atcgcgagca acggggtctt cggtctgttg ctcaacatgc
     2401 tcgtggctga aaaatcgggc ttcaatctgg tcgaccagcc cgccgggtcc gacttgcatg
     2461 atttcaccga aaagatcgcc aaacaggcca tggaagccat ccagcaagcg gtcgccgcca
     2521 acgcgggcac gcttccggca ggtcgtcagg gaaatggaaa tggcgttggc tcgacccccg
     2581 tgcccagcct ggcgactcct accgtcgccc cggtcaccac gcgtgaaacg gcggactcaa
     2641 aagggaaacg gaaggatgga gttcgatcgg gcggcgccgg accggactcg ggcgctgttt
     2701 aattccctta gtccggccaa gcaccagtcg cgtcacggcg gtaagccgag gcgcaaataa
     2761 ctgtgaaatt tagatttgtg aaaaatagcc tgggccgccg cgcgtaaggc ggcgacctcg
     2821 gcatttctgc cctgccagcc aggtctggat cgcatcggtt ccaaggatag tgtacgtgcg
     2881 attccgatgc gggttcaaat gcttgacggc aaataaaccg ggcactaaac tcatgagttc
     2941 ccgccgggtc gaaaagttgt tgtagctggc accattgcgg gcagtaccga atccgcttgt
     3001 cccgtgagcg atgctgtcgt ttttgctcga tcagaggaac tcaggtttat gccgcgtaag
     3061 aagtttggac gcaaccgcaa gaatcgctgt cggttttgca ctcgggatgg ctgccctcgg
     3121 cccgcctatg tcgattacaa agacatcggt ctgctcaaaa agctctgcac caatcagaac
     3181 aagatgtttt cccgcaaacg gagcggcagt tgcgcggcct ttcaacgagc cgccagttcg
     3241 gcggtcaaac gtgcgcgctt catggggctg atgccctacg tgggcgagtg attcgccgat
     3301 ctggccgcac ttcgccggct ggcgcgccgc aaatgagttt tcgagatacc ggggcaccga
     3361 cggtgtaggt cgggctttcc agcccgacgt ccggccggaa agcccgacct acacgcgcat
     3421 aacgtccgga cggaaagccc gaccttctac acgcacataa cgtcgggctg gaaagcgcga
     3481 cctacacgcg cataacgtcg ggctggaaag cccagcctac atgcacataa cgtcgggctg
     3541 gaaagcccga cctactacat gcacataacg tcgggctgga aagcccgacc tacgtgaccg
     3601 ccagtttact gcctcggcac ggtccgaatg gttccaccgc tgggcaatgg ctgagcaact
     3661 gccgctttgc cctgtctctg ctggtcttcg ccgttcctca ggaacgcacg cagctggcta
     3721 tcgatgacgg tatagggctg gctcaggcgt tcttgcaggg atcgccccgg cccgcgcttg
     3781 atccgtccgc gataggcatc ccgaacatag tcgaggaatg catcgcggta ggtgccctgc
     3841 tgccattgca tgagaaag
//
LOCUS       DATAQL010000711         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_377687, whole genome shotgun sequence.
ACCESSION   DATAQL010000711 DATAQL010000000
VERSION     DATAQL010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377687"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VHS97_12380"
     CDS             <1..499
                     /locus_tag="VHS97_12380"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX3449046.1"
                     /translation="HWPVDDLAALGLKLGADVPVFVRGRTAWAEGIGERLTPLFPPQA
                     PFEANYLILKPNVGVSTAEIFQDSELTRNSAPITIHGFLASGGRNDCLGVVRRRYPDV
                     ARAIDWLSDFGPARLTGTGACVFLAVETIDRGREILGKLPPAVDGFLARGLNDSPLLE
                     RLATG"
     gene            541..615
                     /locus_tag="VHS97_12385"
     tRNA            541..615
                     /locus_tag="VHS97_12385"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Gln,seq:ttg)
     gene            803..>1658
                     /locus_tag="VHS97_12390"
     CDS             803..>1658
                     /locus_tag="VHS97_12390"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX3449047.1"
                     /translation="MSQGNEFDSLTIAVFTGKANPALAQEITRHLHVPLGRAHVGRFS
                     DGEVNVELMENVRGRDVFIVQPTNPPAENLMELLMMTDACRRASAKRITAVVPYFGYG
                     RQDRRPRATRVSITAKLVANMIASAGVDRLLTVDLHADQIQGFFDIPVDNVYASPVLL
                     GDAWKQKYKNMIVVSPNVGGVVRARALAKRLDDADLAIIDKRRPRPNESKVMNIIGEV
                     EGKTCVLVDDMVDTAGTLCQAAQALKDEGAIKVVAYITHPVLSGAAIERISKSVLDEL
                     VVTDTIPLS"
BASE COUNT          322 a          425 c          570 g          341 t
ORIGIN      
        1 gcactggcca gtggacgacc tggcggcgct tggactgaag ttgggggcgg atgtgcccgt
       61 gttcgtccgc gggcgcacgg cctgggcgga aggaatcggc gaacgtctga cgccgttgtt
      121 tccgccgcag gcgccttttg aggccaacta tctgattttg aagccgaatg ttggggtgag
      181 cacggcggaa atatttcagg attctgaatt gacaagaaat tctgcgccca tcacaattca
      241 cggcttcctg gcgtctggtg gacgaaacga ttgcctgggc gtcgtgcggc gccgatatcc
      301 cgacgtggcg cgcgcgatcg attggctgtc ggacttcggt ccggcgcgcc tgacgggtac
      361 tggggcctgt gtttttttgg cggttgagac gattgatcgc ggccgcgaaa tcttggggaa
      421 gttgccgccg gcagtagatg gttttttggc tcgcggactg aatgactcgc ccttgcttga
      481 aagattagcg accggctgag aatcggctgt agcgagttcg ctgatcgaaa gatttcaagt
      541 tggggtgtcg ccaagcggta aggcagcggg ttttgatccc gccatacgga ggttcgaatc
      601 cttccacccc agccaaatat ttgcggttgg agcgactggt cggctggaaa tggtcaagtg
      661 caatgagtta gctgggggaa ggataacgcc cgaaggggcg gccccggagg ggtgaggccc
      721 gcagggccga ataatccttc caccccagcc aaatatttgc ggttggagcg attcgtcggc
      781 tggaaatgtt gagctaaggc ccatgtcgca aggtaacgag ttcgattcat tgacgatcgc
      841 cgtattcacc ggcaaggcga atcccgcgct tgcgcaggaa attacgcggc atttgcacgt
      901 gcctctgggc cgggcgcacg tgggacgatt cagcgacggc gaagtgaacg tggaattgat
      961 ggaaaacgta cgcggacgcg acgtgttcat cgtgcagccg acgaatcctc cggcggagaa
     1021 tttgatggag ttgttgatga tgaccgacgc ctgccggcgg gcgtcggcga agcggatcac
     1081 cgcggtggtg ccgtatttcg gctacgggcg ccaggatcgc cgaccgcggg ctacgcgggt
     1141 gtccatcacg gccaagctgg tggcgaacat gattgcgagc gcgggcgtcg accgcttgct
     1201 gacggtcgat ttgcacgcgg atcagattca gggttttttc gacataccgg tcgacaacgt
     1261 gtatgcctcg ccggtgctgc tgggagatgc ctggaagcag aagtacaaga acatgatcgt
     1321 ggtgtcgccg aacgtgggcg gcgtggtgcg cgcgcgtgca ttggccaagc gcttggacga
     1381 tgccgatttg gccatcatcg acaaacgccg gccgcggccg aacgaatcga aggtgatgaa
     1441 catcatcggc gaggtcgagg gaaaaacctg cgtcctcgtc gatgacatgg tggataccgc
     1501 gggcacattg tgccaggcgg cgcaggcgct caaggacgag ggcgcgatca aagtggtcgc
     1561 ctacatcacg catcccgtgt tgtcgggcgc cgcgattgaa cgcatttcga agtcggtatt
     1621 ggatgaactg gtggtgaccg acaccatacc tctgtcgg
//
LOCUS       DATAQL010000712         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4805670, whole genome shotgun sequence.
ACCESSION   DATAQL010000712 DATAQL010000000
VERSION     DATAQL010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4805670"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VHS97_12395"
     CDS             <1..622
                     /locus_tag="VHS97_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449048.1"
                     /translation="HLGNVVRRGYSPTSSEMTWALVDGCFVVADALSLAAVAPEGAVA
                     AESIRSEVKAAVREVTRSAGRELVDSGAESTGKALARHEAEIGLARFSGEGSARLSQR
                     LERWWAVREAGGLYQILRQVPEAQPRLSLAQLAQMAEPLAAKAGMRLSAWRPIRLLRN
                     GIEVVLSIPPQRGLKYLAAQAAQAGVGVVGFQKMEEHLASRRPLGS"
     gene            759..1772
                     /locus_tag="VHS97_12400"
     CDS             759..1772
                     /locus_tag="VHS97_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449049.1"
                     /translation="MKHNAEGRRGWGAVVDSGRLAGMIAVAMVLLSHPGSARAGKLSW
                     LDDLVQEVIIEAKAGGKGLARGSDGARTEIRGAGRLFLAHNADETLERLVRSSDELAQ
                     AGRRVEQPTEALLQARFSRLLKQDPQALRAFGALEPAEKRLVVELGETAQNLARRYPE
                     QAETMVRHLGPEGLTAVRVFGDDVAEVLAKEGSQSLGVLRKTGRGGWSFFTNQVLPHK
                     KKLAAAGILAVFLANPEKFVDYTGQATEFAVREFAKAGIQLARAVGGGAARGLETTIG
                     NTLASYGLDFPLFRYAGIGLAGLVVVLSVLVIVGRPIRLIFRPFGWAFRKTATLGSLV
                     KKA"
     gene            1832..>3665
                     /locus_tag="VHS97_12405"
     CDS             1832..>3665
                     /locus_tag="VHS97_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449050.1"
                     /translation="MGMNNRRTDRLNALEARLSGPKRIALFGHRNVGKTTLLAMFYRQ
                     ASSGRVPGLRLAATDPKSAEYLAEKIAQIETGQPMAGSLAETQLNLRLYHGPARFELI
                     IKDYQGEHITLGSEEPIQEFFADCDAVFLCLDPEGSTEPAERRRRQQEIENLLERYID
                     RSEDLSMGRPVALLLTKFDRVLANDSSGSQVAGTQGSLSTEFVERIVDERYGMTRHAL
                     AEHAPDGAIFAVSSFGEDAIGNRPPAELRPLGLEGPLGWVAEQLEARDRADMSSLWEE
                     APNELPRLERCVAAYERRYPRSNRSYEFRGRLKKQLRRRRWRWAMRGAAAAILAAGML
                     AGSDLLAFYQASSFERAPDSAAPAVARRWSELLERHPWLPAFWPALAREARQKKGEWQ
                     VKAADVQIANGTAPADLTARLGQLKDQRPQLVPEIKKVEAAQEQARHDERWKSVQAEA
                     LSLAAIDDPATPLATIDAFLRAFPETPRRAEALSLARSLKNEIATKRSAAERRIVEDL
                     IHSESLPNVSLADQIERARQFIAEHPDSPVRPEVQRRLDIYLEKLDERDIEKARDYSR
                     QNSTQFATRIDRYQDYLKAHQAGGGFISEAIEAKDRILREWDTYA"
BASE COUNT          716 a         1109 c         1204 g          636 t
ORIGIN      
        1 tcacctgggc aacgtggtgc ggcggggcta ttcaccgacc tcgagcgaga tgacctgggc
       61 gcttgtcgac ggctgtttcg tggtcgccga cgccttgagc ctggcggccg ttgcacccga
      121 gggagctgtg gccgccgagt caatccgttc cgaggtcaag gcggcggttc gtgaggtgac
      181 gcgatctgcg ggacgcgagc tagtggattc cggagccgaa tcgactggca aagcattggc
      241 tcgccatgag gctgaaatcg gtctcgcacg cttcagcgga gaagggtcgg cccggttgtc
      301 gcagcgtttg gagcggtggt gggccgtccg ggaggcaggt gggttgtacc agatcttgcg
      361 gcaagtcccc gaggctcaac ctcggctcag cctggctcag ctggcgcaga tggccgagcc
      421 gctcgccgcc aaagccggca tgcgcttgag tgcgtggcga ccgatccgac tgctgcggaa
      481 tggtatcgag gtggtgctaa gcattccacc gcaacgaggg ctgaaatacc tggccgcgca
      541 agccgctcag gccggcgtgg gtgtggtcgg ctttcaaaag atggaagagc acctggcctc
      601 acgccgaccc ctaggttcct gaactggatc agacgtatgg cggtaacact ggcgacgcag
      661 ttccgggaag acacacaaca atgccatcag tttgccgaat agccagggag agagccgcaa
      721 tgtcacactg tcgtccggaa gcgcaaggat ttgagtccat gaaacacaat gccgaaggcc
      781 gccgtgggtg gggagcggtt gtcgattctg gacgcctggc tgggatgatc gcggtggcaa
      841 tggttttgct gagccacccg ggctcggcta gggcaggaaa actgtcctgg ctcgacgacc
      901 tggtgcaaga agtgattatc gaagccaagg caggcggaaa aggattggct cgcgggagcg
      961 acggcgcgcg aacggagatc cggggcgcag gccggctgtt cttggctcac aacgccgatg
     1021 aaacgctcga gcgattggtc cgcagctccg acgagctggc acaggccggc cgacgggtcg
     1081 agcaacccac tgaggctctc ctgcaggctc ggttttctcg actgctcaag caagacccac
     1141 aagcgcttcg cgccttcgga gcgctcgagc ccgccgaaaa gcgactggtc gtggagctgg
     1201 gtgagaccgc gcagaattta gcgcgccgat accctgagca ggcagagaca atggtgcgac
     1261 acctcggacc cgaaggactg actgccgtcc gggtctttgg agacgacgta gccgaagttc
     1321 tggccaaaga gggatcacag agcctgggtg tgttgcgcaa gaccggccga ggcggctggt
     1381 cctttttcac caatcaagtt ctgccccaca agaaaaagct ggcggccgcg ggcatcctgg
     1441 cggtctttct ggccaacccg gaaaagtttg tcgactacac cggccaggct accgagtttg
     1501 ccgttcgcga gtttgccaag gcgggcatcc agcttgctag ggcggtcggt ggtggagcgg
     1561 cgcgggggct ggaaaccacg atcggcaaca ccctggcctc ctatgggctc gacttccctt
     1621 tgtttcgata cgccggcatc ggtctggctg gactggtcgt agtgctttct gtactcgtga
     1681 tcgtcggacg accgatacgg ttgatatttc ggccgttcgg atgggcgttc cgcaagacag
     1741 cgactcttgg gtcattggtg aaaaaggcct gacacgaacg accgcgcata ctctttggtt
     1801 ggcgctcgcg gctgccggag ggatctgagg catgggaatg aacaaccgcc ggaccgaccg
     1861 gctcaatgcc ctggaagcgc ggctcagtgg gccgaagcgg atcgcgctat ttggccatcg
     1921 caacgtgggg aagaccacgt tgctggcgat gttttaccgc caagcctcga gcggcagagt
     1981 gcccggcctg cgactggcgg ctaccgaccc gaaaagtgcc gagtatctgg cggaaaagat
     2041 cgctcagatc gagacgggcc agcccatggc gggctcgctc gccgagaccc agctcaacct
     2101 tcggctctat catgggcccg cacggtttga gctgatcatc aaggattacc agggggagca
     2161 catcacgctc ggcagcgagg aacctattca agaattcttc gcggattgcg acgccgtctt
     2221 cctctgtctc gaccccgaag gctcaactga accggccgaa cgacgacgac ggcagcaaga
     2281 gatcgaaaac ctgctggaac gctacatcga tcgatcggaa gacctgagca tgggtcggcc
     2341 ggtcgcactg ttgttgacca aatttgatcg cgtcctggcg aacgattcgt caggatccca
     2401 agtcgccgga acccaaggca gtctgtccac ggagttcgtt gagcggatcg tcgacgagcg
     2461 atacgggatg acgcggcatg ccctcgcaga gcatgcgccc gacggtgcga tctttgccgt
     2521 gagctcgttt ggcgaggatg ccattggcaa ccggccccca gccgagctga gaccgttggg
     2581 cctggaagga ccgctgggct gggtagccga gcagctcgaa gcacgcgatc gcgcggacat
     2641 gagctcgctc tgggaggagg cacccaacga gttgccccgg ctggaacggt gtgtcgccgc
     2701 atacgaacgg cgctaccctc ggtcaaaccg gtcgtacgag ttccgcggac gtctcaaaaa
     2761 gcagttacgg cggcggcggt ggcgctgggc gatgcgtggg gcggccgcgg cgatcctggc
     2821 ggccggcatg ctggccggct ctgacttact ggccttttac caggcatcct cctttgaacg
     2881 agcccctgat agcgccgctc ccgcggtcgc tcgccgctgg tccgagctgc tggaacggca
     2941 tccttggctt cccgcattct ggccggccct ggccagagaa gcccgacaga agaaaggcga
     3001 atggcaagtc aaagcggccg acgtccagat cgctaacggc accgcgccgg ccgatctgac
     3061 cgcccggctc gggcagctca aggaccagag accgcagttg gtccccgaga tcaagaaggt
     3121 ggaagccgcc caggaacagg cgcgtcacga tgagcgatgg aaaagcgtgc aggccgaggc
     3181 cctatcgctg gccgccatcg acgaccccgc gactccgctc gccacgatcg acgctttctt
     3241 gcgagcgttc cccgaaactc cccgccgggc cgaggcgcta tccctcgctc ggtcgctcaa
     3301 gaacgagatt gccaccaagc ggtccgcggc cgaacggcgg atcgtcgagg acttgatcca
     3361 ttccgaatcg ctgcccaacg tctcgcttgc cgaccagatc gagcgcgcac gccagttcat
     3421 cgccgagcat ccggatagtc ctgttcgccc ggaggtgcag cgccggctgg acatctacct
     3481 tgaaaaactc gatgaacgcg acatcgagaa ggctcgcgac tactcccggc aaaactccac
     3541 tcaatttgcg acccgcatcg accgctacca agattacttg aaggcgcacc aggccggtgg
     3601 cgggttcatc agtgaggcga tcgaggccaa ggatcgcatc ctccgtgaat gggacaccta
     3661 cgcct
//
LOCUS       DATAQL010000713         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_834982, whole genome shotgun sequence.
ACCESSION   DATAQL010000713 DATAQL010000000
VERSION     DATAQL010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834982"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(591..1496)
                     /locus_tag="VHS97_12410"
     CDS             complement(591..1496)
                     /locus_tag="VHS97_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3449051.1"
                     /translation="MKHVSNLLVLARRGPLVVLIASALALVTLPASASGLVRSVASQH
                     STHARLSDGLQVTKPTIVLVHGAWADASSWGRVTQILQDEGFTVVAPPNTLRGISHDS
                     AVLANYLKTIAGPIVLVAHSYGGAVVTNAATGNPNVKQLVYVDAFMPAQGETIGQLLS
                     QMPGSCVLANPSDAFTLSTDPSQPAGDPDAYLKGPASGNYMGFDQCFANSVAPKQAAL
                     LEAGQRPIAVGALSEPSGAPAWSTIPSWALIGSEDHVIPPAELTFMAKRAGSRIEYVK
                     AGHVSMVAQPLQVAKVITQAAQAAS"
     gene            complement(1553..2374)
                     /locus_tag="VHS97_12415"
     CDS             complement(1553..2374)
                     /locus_tag="VHS97_12415"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3449052.1"
                     /translation="MSFITTEDGAEIFYKDWGEGPVVTFSHGWPLNADAWDRQMLFLV
                     HNGYRCIAHDRRGHGRSAPSFSHNDMDGYADDLAAVIDALDLRDATLVGHSTGGGEVA
                     RYIGRHGTERVAKTVLLSAVPPIMVQSDSNPEGLPLKVFDEMRASVARERSQFYRELA
                     IQFYGANRPDANVSQSLLDEFWLLSMQAGALNAYLSIKAFSETDFTEDLKKMDVPTLV
                     MHGEDDQIVPVHDSARKSARLIRGASETYYPGASHGITATLQDQVNNDLLEFIRS"
     gene            2620..>2823
                     /locus_tag="VHS97_12420"
     CDS             2620..>2823
                     /locus_tag="VHS97_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449053.1"
                     /translation="MANHECAECVGPSLNREGKSYLLDPVVAAEVAAAHLEAPAVGNA
                     LALRAYAKLLAESDRLFQLIIDGR"
BASE COUNT          584 a          835 c          830 g          574 t
ORIGIN      
        1 gtcgggtact ccacgatcga caagcgcatt cgttatggcc ctgaaccatt gaatgcagcc
       61 tctctcaacg tccactgcaa ggcacggatg gagccttccg acccgtcgac gccgaccacg
      121 atgcgtcctg tcttccttcc cagcacttgc ctccatgtga ctcatgccag acctccttgt
      181 ctcgacaacg acggtgaccg ggagcggccg aatccgaggt ccagttggcc gtaagtcgtt
      241 gacgggcatc gccagtcgaa tgcagggcgg ggttatggtg caatttggag gtgagacgca
      301 cgacaacgtg gaggcccgcg ttatctgcgc cggcaaacat aatccgacct ccggtaatga
      361 cggacgactt ggaagctgcg ttctccgcag ggctatcggt cgtctcgccg aatacaaagg
      421 ctcggttccg ctcgacctcg aagtcctgag ccgcccgttc tatgacctga ccaactgatc
      481 gatggagttg gttcgattct tcaggatcga atatgacacg acctcatagg aaatggttcc
      541 cgagaccgga ctgatcgcac cgaagtgcga ccagtccgat cgggatacgt tcaactcgct
      601 gcttgggctg cttgagtgat gacctttgca acctggagcg gctgtgcgac catcgagaca
      661 tgaccagcct tcacgtactc gatccgtgaa ccggcgcgct tagccataaa ggtgagctcg
      721 gccggaggaa taacgtgatc ctctgaaccg atcagcgccc aggacggaat agtcgaccac
      781 gctggtgcgc ctgatggctc gctgagtgct ccaaccgcaa tgggccgctg tccggcttcc
      841 agcaatgcag cctgcttagg cgccacggag ttggcgaaac actgatcgaa tcccatgtaa
      901 ttgccgctgg caggtccctt caggtaggcg tccggatctc cggccggctg ggaagggtca
      961 gtcgagagcg taaaagcgtc gctggggttt gccaagacgc atgatccagg catctgcgac
     1021 aagagctgtc caatcgtctc cccttgtgca ggcatgaacg cgtcgacata cacgagctgt
     1081 ttgacgttcg ggttcccggt tgcagcgttc gtgaccaccg caccgccgta cgaatgggca
     1141 accaacacga tcggacccgc aatcgtcttt aggtagttcg caagtacggc cgaatcgtgg
     1201 gaaatgcccc gcaatgtgtt cggcggcgcg acgacggtga agccctcatc ttgaaggatc
     1261 tgagtcaccc ggccccagct cgaagcgtca gcccatgccc cgtgaacgag aacgatcgtc
     1321 ggcttcgtga cttggagacc atcggaaagg cgcgcgtgcg tcgagtgctg ggaggcaacg
     1381 ctacgcacaa ggccgctcgc agacgctggc agagtaacta gagccaacgc gcttgcgatc
     1441 aagacgacca gcggtcctcg cctggccaga acaagcagat tactcacgtg tttcatctct
     1501 gtatcctttc ttgagccacg ccgtccgaat gacatgcgtg gcggcttggc tgctagctgc
     1561 ggatgaactc cagcagatcg ttgttgacct ggtcttgcaa tgtcgccgtg atgccgtgcg
     1621 atgcgccggg gtaataggtc tcgctcgcac cccgaatcaa ccgcgcggac ttacgcgctg
     1681 aatcatggac cgggacaatt tggtcgtcct ctccatgcat gacgagagtc ggcacatcca
     1741 tcttcttgag gtcctcggtg aagtcggtct cagaaaatgc tttgatcgag agataggcgt
     1801 tgagagcacc agcctgcatg ctcaagagcc aaaactcatc cagcaagctc tgggaaacgt
     1861 tggcatcggg gcgattggcg ccgtaaaact gaatcgcaag ttcccggtag aactgcgaac
     1921 gctcgcgtgc cacgctggcg cgcatctcat cgaagacctt caagggcagg ccctcagggt
     1981 tcgagtcgga ttggaccatg atgggcggca cggcggaaag caggacagtt ttggcaacgc
     2041 gctcggtacc atgccggccg atgtagcggg ctacctcacc accaccggtt gaatggccca
     2101 ccaacgtggc gtcgcggaga tcgagagcat cgatcacggc cgccagatcg tctgcgtagc
     2161 cgtccatgtc gttgtgcgaa aatgacgggg cggaacgtcc atggccacgc cggtcgtggg
     2221 cgatacagcg gtaaccgttg tggacgagga acagcatctg acgatcccag gcgtcagcgt
     2281 tcagcggcca accatgtgag aaggtcacga ctggcccctc gccccagtcc ttgtagaaga
     2341 tctcggcgcc atcctcagtt gtgatgaaac tcattagccg ctcctttcaa atcgcggatt
     2401 tgaagatttg atttcggcga acccctgatc gcgttgagaa ctctgatctg aggctgcctc
     2461 gtacgcatcc tctgctttgt ggtgccccct cgcatctgtc gaactcccta gtggcgttat
     2521 gggcgaatga gatccccagg cggtgcgtcc caggactggt cggttctacc gactcaaccc
     2581 gcgtcggaag cacagacaat tcaatagatt gaacctgtga tggcgaatca tgaatgcgcc
     2641 gaatgtgtcg gtccgagctt gaatcgggag ggcaagtcat atctgctcga tcccgtcgtg
     2701 gcggctgagg tcgctgcagc tcacctcgaa gcgccggccg tcggtaatgc tctcgcctta
     2761 cgcgcgtacg ccaaactgtt ggcggaatct gatcgcctct tccagctcat catcgacggg
     2821 cgt
//
LOCUS       DATAQL010000714         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2821657, whole genome shotgun sequence.
ACCESSION   DATAQL010000714 DATAQL010000000
VERSION     DATAQL010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2821657"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VHS97_12425"
     CDS             <1..224
                     /locus_tag="VHS97_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449054.1"
                     /translation="RKWFETERNNAFLERTRIGRFNLRRLTVALAHHPSPLPREREPV
                     TCIGREFAVGASGLAGNRPYFRPVTCIGR"
     gene            complement(304..1119)
                     /locus_tag="VHS97_12430"
     CDS             complement(304..1119)
                     /locus_tag="VHS97_12430"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX3449055.1"
                     /translation="MTLYWIYDLPNWMLGTLIVTAFVGLALVGLFLSRPIVGRLLGYS
                     PKHNDVVSYFFSGMGVFYGLAMGLIAVATWENYTEIDGVVSTEAAAVASFYRDLDGYP
                     QPVRMQLEATMRDYTRTVIETEWPAHKKGVALENGDAILDRLENMLMAFEPTKEREKI
                     SHAEVLRSWNTLIEQRRLRLQAVTTGLPTALWAVVLIGALLNGLLTYLFWVENLWLHA
                     SLVVILSVFIALLVFLTAAMDNPFRGEFSVSSEAFQTILEKVMIPGETASGKE"
     gene            1456..>3933
                     /locus_tag="VHS97_12435"
     CDS             1456..>3933
                     /locus_tag="VHS97_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449056.1"
                     /translation="MSTERLTETMEKTKETPPKVRASSRGEAPSSSSWSARLADVCLI
                     VLFLILTCLLGTFPLKDADIYWHLRTGDLIRQSGQIPRTDIFTFTREGVPWIDLHWLF
                     QIAISWVHQHGGVVGLNVAKCLVTCAAVLLLVSARRRDWPVWAMVLAWIPALFVLGGR
                     MYVRPETLTLLYLSIFLAVISRWDRFPRLTWILPFVQIAWVNSHGLFVLGPIIVIFAL
                     IDAALRFGIFGAERRKWWRTILAASLATGAACLVNPYFIAGALYPIELAGTMRNPIFS
                     KHIAELQSIPDFLNIAEVKTIPDFLRSAGINNLPMQLHLLAMVLGGLSFILPLIWLVR
                     VRLAATGANLPDGSDATRDVRQTESVGKKRRSKVAESKAGKTRQRNTTEIVQAPLWRL
                     SPFRILLYVAFCILSLQATRNSHQFAAVVGTVTAWNFGEWAAAIRRRRMASAAVRSNS
                     GGAVPRLVAFGTVVLLIMCVGSGLFFKMTGEGRVINLGEEPLFFPHEATRFAGEGEMP
                     KRFLSFHNGHASLFEYYHGPGSKVYTDPRLEVAGPELFTRYIELERRLEQDGPGWEVE
                     LGEMGRPVILTDHEHNASIGATLLQSNHWRCVWFDAIAAVFVHDSYAAVVQAHAIDFA
                     AQHFRPDSRPKPRDLAERVALANAYRKYLMTLSRTAPERARPLVWLGLDDARSILRQE
                     PGSFEGWKNLGQIELNRDVAVAPNPRFRMPFDPVFDLSLIRATYALRRALEVVPTDFM
                     TLVVLKSAYDARLMSEAGLPVVDQIARIQTKNSYQAKLQALAGPARVEYLQKMGPSPA
                     TSWANLSALDQIVTTLLASGRAESAAAR"
BASE COUNT          770 a         1140 c         1186 g          837 t
ORIGIN      
        1 aaagaaagtg gtttgagacc gagcgcaaca atgccttttt agaaaggacg cgcatcgggc
       61 gattcaactt gagaaggctc acggtcgcgc tcgcccatca ccccagccct ctccccaggg
      121 agagggagcc agtaacatgc atcggccgcg agtttgctgt cggggcgtcg ggtttggccg
      181 gcaatcggcc gtatttccgg ccagtcacat gcatcggccg ctagcgtgct gtagcggggc
      241 ctttgggcct ccctggcagg tgtcagaaaa acccctcctt ctaagcgcgt agggacggcg
      301 cgatcattct ttgcccgacg cagtttcgcc gggaatcatg accttttcga ggatcgtctg
      361 gaatgcctcc gacgacacgc tgaactctcc ccggaacggg ttgtccatcg cagccgtcag
      421 aaagaccaag agcgcaatga agaccgacag gatgacgacc aggctggcgt ggagccaaag
      481 attctccacc caaaacagat aggtcaagag accattgaga agagccccga tcaagacgac
      541 ggcccagagc gcagtcggca gacctgtcgt aacggcttgc agccgcaacc gccgttgttc
      601 gatgagcgtg ttccaggagc gcaagacctc ggcgtgggat atcttttctc gttccttggt
      661 cggttcgaag gccatcaaca tgttttcgag acggtccaga atcgcatcgc cgttttcgag
      721 cgcgactcct ttcttgtggg cgggccactc ggtctcgata acggttcggg tgtagtcgcg
      781 catcgtcgct tcgagctgca tgcgcacggg ctgcgggtag ccgtccaagt cgcggtaaaa
      841 gctggcgacc gccgccgcct ccgtcgatac gaccccatcg atctccgtgt aattctccca
      901 ggtagcaacc gcgatcaagc ccattgctaa gccgtagaaa actcccatgc cgctgaagaa
      961 gtaactgacg acgtcgttat gcttgggcga atagccgagc agccgaccga cgatcggccg
     1021 gctgaggaac agccccacga gcgccaaacc gacgaaggcc gtgacgatca gggtacccaa
     1081 catccagttt ggtagatcgt agatccaata cagcgtcatg agctgaccct ccgtgggaac
     1141 ccggatcgca tttcatcgcc ttcattgccc gtcgcgcggc ggagaagtcc cgtagggatc
     1201 tcgatccgtg cgtgcgtgcg tcgtaagtcg tcgattgatt tgctgaacat aacgttttgc
     1261 attgcgtcga accatgccga ttcgcgagag ctgcgtctca agctcgatgc cgattcgggc
     1321 tggatgaatc ggtcgcgcgc atcgaaaatg aatgagtcga gcggaccaag gaatcgcgga
     1381 tcgagtcgac agtgcggtcg tggctctcga gttgtgttcg ggcgccggct cgcaaacgtt
     1441 tgtcggagtg tccagatgtc gaccgaacgc ctcacggaaa cgatggagaa aacgaaggaa
     1501 acgccgccga aggtacgagc ctcgagccga ggtgaggcgc catcatcatc gtcgtggagt
     1561 gcccggctcg cggatgtgtg cttgatcgtt cttttcctga tcctgacgtg cctgctcgga
     1621 acgtttcccc tcaaggatgc cgacatctac tggcacctgc ggacgggcga tctgattcga
     1681 caaagcgggc agataccgcg gaccgacatc ttcacattta cgcgcgaagg cgtgccctgg
     1741 atcgacctgc actggctttt ccagatcgcg atcagctggg ttcatcaaca cggtggtgtc
     1801 gtcgggctta acgttgctaa atgcttggtc acgtgtgcgg cggttttgtt actcgtgtcg
     1861 gcgcggcgac gcgattggcc ggtttgggcc atggtcttgg cctggatacc tgctctattc
     1921 gttctgggag ggcggatgta cgtccgcccc gaaacgctga cgctgctcta cctgtcaatt
     1981 ttcctggcgg tcatctcgcg ttgggacagg tttcctcgac ttacgtggat attacccttc
     2041 gtgcagatcg catgggtgaa ttcgcacggt ttgttcgtcc tcgggccaat catcgtgatt
     2101 tttgcgctga tagatgcagc gctgcgattc gggatattcg gggctgagcg gcggaaatgg
     2161 tggcgaacga tactcgccgc gagcttggcg acaggtgccg cgtgcctggt caatccttac
     2221 tttattgcgg gcgcccttta tccgatcgag ctcgctggaa cgatgcgaaa cccgatcttt
     2281 tccaagcata tcgccgagtt gcagtcgatc cctgacttcc tcaatatcgc cgaggtgaag
     2341 acgatcccag acttcctcag gagcgcgggg ataaacaacc tgccgatgca acttcatctg
     2401 ctggccatgg tcctgggtgg tctcagtttc atacttcccc tgatttggct cgtgcgcgtc
     2461 cggctggcgg cgactggagc gaacttgcct gacggtagcg atgcaacccg cgacgttcga
     2521 cagaccgaat cggtgggaaa aaagcgtcgc tcgaaggtgg cggagtcaaa agcgggaaag
     2581 acgaggcaaa gaaatacgac agaaatcgtt caggcaccgc tttggcggct cagtcccttc
     2641 cggatcttac tctacgttgc cttttgcatt ctgagcctgc aggcaacacg caacagtcac
     2701 cagttcgccg cggtggtcgg cacagtgacc gcctggaatt ttggcgaatg ggcggccgcg
     2761 attcgacgcc ggcggatggc cagcgcggca gtccggtcaa attccggcgg tgccgtaccc
     2821 cgtctagtcg ctttcggaac ggttgtattg cttatcatgt gcgtcgggtc gggcctcttt
     2881 ttcaagatga ccggcgaggg ccgcgtgatc aatctgggtg aggaaccgct gtttttcccc
     2941 cacgaggcca cccgattcgc gggcgagggc gaaatgccca aacggttcct ttcattccac
     3001 aacggacatg cgtccctctt cgagtattac cacggcccgg gaagcaaggt ttataccgat
     3061 cctcgactgg aagtcgccgg acctgagctc ttcacgcggt acatcgaact cgagcgccgt
     3121 cttgagcagg atgggcctgg gtgggaggtc gagctcgggg agatggggcg tcctgtcatc
     3181 ctgaccgatc acgaacacaa cgcgtccatc ggcgccacgt tgcttcaaag caaccattgg
     3241 cgctgcgtct ggttcgacgc gatcgccgcg gtcttcgttc acgactcgta tgctgcggtt
     3301 gtccaggctc acgccatcga ttttgcggcg cagcattttc ggccggacag ccgtccgaag
     3361 ccgcgggact tggccgagcg ggtcgccttg gccaacgcgt accgcaaata cctgatgacg
     3421 ctgtcgagaa ccgcgccgga gcgggcccgc ccattggtat ggctggggct cgatgacgcc
     3481 cggagtattc tgcgtcaaga gcccggatcg ttcgagggct ggaaaaacct gggccagatc
     3541 gaattgaacc gagacgtggc cgttgcgccc aaccctcgtt ttcgcatgcc atttgatccg
     3601 gtttttgatc tatcccttat ccgcgcgacc tatgcacttc ggcgtgcgct cgaagtggtt
     3661 ccgaccgact tcatgacgct cgtggtgctg aaaagtgcat acgacgcgcg cctaatgagt
     3721 gaggctggtc tgccggttgt ggaccagatt gccaggattc aaaccaaaaa ttcctatcag
     3781 gccaagttac aggctctggc cggtcctgcg agggtcgaat atttacagaa aatgggtcca
     3841 tcccccgcga cttcgtgggc caacctgagc gcgctggacc agatcgtgac caccctgctt
     3901 gcctcggggc gagccgaaag tgcggccgct cga
//
LOCUS       DATAQL010000715         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2821692, whole genome shotgun sequence.
ACCESSION   DATAQL010000715 DATAQL010000000
VERSION     DATAQL010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2821692"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VHS97_12440"
     CDS             <1..213
                     /locus_tag="VHS97_12440"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX3449057.1"
                     /translation="EVRDAALIAAAQRVEHYEIAAYGSARTFAEQVGQRDIAQLLQQT
                     LEEEYMADEKLTKIAESSVNKSAARM"
     gene            557..1276
                     /locus_tag="VHS97_12445"
     CDS             557..1276
                     /locus_tag="VHS97_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX3449058.1"
                     /translation="MPVNLQWQEIALRLVLTVVAGAVFGLNRSEHGRPAGLRTTLLVC
                     LAASIAMIQTNLLLATSGKPSNSFVTLDLMRLPLGILSGMGFIGAGTILRRDNLVQGV
                     TTAATLWFVTVIGLCLGGGQIVLGSTAVGLGLIVLWGLQWVEERLPQDRYSTLTLTLG
                     LGRPTDEEVYETIRAAGLRVVVQGAYYDATTERRELRCEVRWRAQSADTRFPSVVQEL
                     SRLPGVASLRWTPQGTPAGAG"
     gene            1279..>2438
                     /gene="ilvA"
                     /locus_tag="VHS97_12450"
     CDS             1279..>2438
                     /gene="ilvA"
                     /locus_tag="VHS97_12450"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX3449059.1"
                     /translation="MQEYSSQGSNPFLLTLQGTPASPLGLSDYLQKILTARVYDVAVE
                     SPLELAKKLTERLGNRVWLKREDSQPVFSFKLRGAYNKMARLSPEQLDRGVICASAGN
                     HAQGVALSAERLNCRSVIVMPVTTPKLKIDAVRSYKGDVVLHGDSYTEAYLHALELSK
                     QRSYTFVHPFDDPDVIAGQGTVGMEILRQHQHPIHAVFVAIGGGGLISGVAAYLKAVR
                     PEIKVIGVQMADSDAMIRSIRAGKRVELHDVGLFCDGTAVKLVGEETFRMTQALVDDF
                     VVVDTDAVCAAIKDVFEDTRSILEPAGAMGVAAMKQYVGRHDIKGVSLVTITCGANMN
                     FDRLRFVAERAEVGEEREALFAVTIPEQRGSFKHLCEIIGERSVTEFNYRIS"
BASE COUNT          481 a          684 c          789 g          484 t
ORIGIN      
        1 gaggtcaggg acgcggcatt gattgcagcc gcccagcgcg tggagcacta cgagatcgcg
       61 gcatacggct cggcccgcac gttcgccgag caggtcggcc aacgcgatat tgcgcagctt
      121 ttgcagcaaa ctctggaaga ggaatacatg gctgacgaga agctgacaaa aatcgccgag
      181 tcatccgtca ataaatccgc cgctcgaatg taaatccggg attcgctcac cagacgagtt
      241 tcacgaagtg gagagaggca tgcggaaagt ccgcatgcaa gtctccactt tttttgtttc
      301 gatcgatcag gtggccccca cagaaggatg tcgttggccg cacatccgcg gaccaatggc
      361 aagaccgcgt cggctgtgct tgctcggggg ggcgattgaa gtaaatcctg gaaactgcgt
      421 gcggacgtcc catataatgc gagggtcgct tcccatccat gcgagataga gatcgctgca
      481 agaaagctcg gttgctggtc gcagcgcggt cggaatgtcg catgacgcaa tgcggcggca
      541 ttcgtgtagg aacgccatgc ccgtcaatct tcaatggcaa gaaatcgctc tgcggctggt
      601 cctgacggtc gttgccggcg cggtgttcgg gctcaatcgg agtgagcacg gccggcccgc
      661 cggcctgcga acgactctcc tcgtgtgtct ggctgcttca atcgcgatga tccagaccaa
      721 cttgctgctt gcgacatcgg ggaaaccgtc gaactctttc gtgacgctcg atctcatgcg
      781 attgccgctg ggaatccttt cagggatggg cttcatcggc gccgggacta ttctgaggcg
      841 ggataacctg gtccagggcg tcacaaccgc ggcgacgctc tggtttgtga ccgtcatcgg
      901 cctttgcctg gggggtggtc agatcgtcct gggctcgact gcagtcgggc tcggtctgat
      961 cgtgctctgg ggcctgcaat gggtcgagga acggctgcca caggatcggt attcaacgct
     1021 caccctgacg ctcggtttgg gtcgaccgac cgacgaggag gtctatgaga cgatacgagc
     1081 cgcgggactc cgcgttgtcg ttcagggcgc atactacgac gcgacgaccg agcgacgcga
     1141 gctccggtgc gaagtacgct ggagggccca gtctgccgat acccgatttc cgtccgttgt
     1201 gcaggagctg tcgcgactcc ccggcgtcgc aagtcttcgg tggactccgc agggaacccc
     1261 agcgggcgcg ggataagcat gcaggaatac tcgagccaag gaagcaatcc gtttttgctg
     1321 acccttcaag gaacaccggc atctccgctc gggctctcgg attacctaca gaagatcctg
     1381 acggcgcgcg tttatgacgt ggcggtggaa tctcctctcg agctcgccaa gaaactgacg
     1441 gaacggctgg gcaatcgcgt ctggctcaag cgcgaagaca gccagccggt attcagcttc
     1501 aagttacgcg gggcctacaa caagatggct cggctctcac ccgagcaatt ggatcggggt
     1561 gtgatctgtg cttcggcggg caaccacgcg cagggagtcg ccttgagcgc ggagcgattg
     1621 aactgccggt cggtcattgt gatgccggtc acgacgccga aattgaagat cgacgcggtg
     1681 cgctcgtaca aaggagatgt ggtcctgcac ggcgacagct acaccgaagc ttacttgcac
     1741 gctctcgagc tgagcaaaca gcgcagttac acgtttgtgc acccgtttga tgatcccgat
     1801 gtgatcgccg gccaggggac ggtcggaatg gagatcttgc gtcagcatca gcacccgatt
     1861 cacgcggtgt tcgtcgcgat cggcggcggt ggtctgattt cgggtgtggc cgcttacttg
     1921 aaggccgttc ggccggaaat caaggttatc ggcgtgcaga tggccgactc cgatgccatg
     1981 attcgctcga tcagggcggg caagcgcgtc gagctgcacg atgtcggcct cttctgcgat
     2041 ggcacggccg tcaaactggt gggcgaggaa actttccgca tgacgcaggc gctcgtcgat
     2101 gactttgtcg tggtagacac cgacgcagta tgcgcggcga tcaaggatgt gttcgaggat
     2161 acgcgcagca tcctcgaacc ggccggcgcg atgggcgtgg cggcgatgaa gcaatacgtc
     2221 ggacggcacg atatcaaggg tgtgtcgctg gtgacgatca cctgcggggc caacatgaac
     2281 tttgaccggc ttcgtttcgt cgccgagcgc gccgaggtcg gagaggaacg cgaggccctg
     2341 ttcgcggtca cgatcccaga gcaacgtggc agtttcaaac atctgtgcga gattatcgga
     2401 gagcgtagcg tgaccgagtt taactaccgg atctccga
//
LOCUS       DATAQL010000716         5276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_989890, whole genome shotgun sequence.
ACCESSION   DATAQL010000716 DATAQL010000000
VERSION     DATAQL010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989890"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VHS97_12455"
     CDS             <1..295
                     /locus_tag="VHS97_12455"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX3449060.1"
                     /translation="DEHVARLESAIDTLETELKPLNQFILPGGTLAAAQIHLARTICR
                     RAERLTVKLSRQLGEDVPSTLIVYLNRLSDFLFVLARAVNHRADVPDIPWKGL"
     gene            297..1085
                     /locus_tag="VHS97_12460"
     CDS             297..1085
                     /locus_tag="VHS97_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3449061.1"
                     /translation="MATSAERTMAQSLQDKVVLITGGRRVGGELALLMAAQGWRVAMT
                     YHTSRAAIERIIKSVEEEGTEGLAIAADLARPEQAQNAVNQVVARFGRLDALVNMASV
                     FRRTPLADLSARDFDDMIAGNLAAPYHTALSAARQMLKQPEIDGIKGKIVVIGDWATD
                     RPYKDYLPYLVAKGGLKTMTLALAKELAPHITVAMIQPAMIDPPPQLTDDDKAAVVAA
                     TPLRRVGSPDDVNRLILYLLEGTNFVTGACFRVDGGRFLGEDSC"
     gene            complement(1154..3070)
                     /locus_tag="VHS97_12465"
     CDS             complement(1154..3070)
                     /locus_tag="VHS97_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX3449062.1"
                     /translation="MSRRRPLSVIPLVIAGFLPLIEPSIRAVRAQSPRVGTRAPEFGV
                     VVEKNVMVSMRDGVHLAADIYRPARDGNVAPARFPTLLTRTPYNKDGAGAEGRYYAER
                     GYVVVANDVRGRYASEGTWRLIDDDPQDGYEIVEWIARQDWSDGKVGTFGTSYPGGTQ
                     HALAEMKPPHLTTMVPIDAVSNCAVSGMRHGGAFELRFMNWIFQTGAPNSKAALADPA
                     LRRTLIENGLRIRQHVDNLPLRMGTTPLKVVPEYEAWLVEAMRSGPESPFWHKKGMSV
                     VDHLNDYADVPVLHMTGWYDSWTRQVTMNYQSLSKAKSSPQRLVIGPWVHGGQTAYTA
                     GEVEFTKEAGIDLLAFRLRWYDRWLKGDKNGVDDDAPVLLYIMGTGDDRRSPAGRLQH
                     GGFWRAEREWPPARVAPTSLYLNADGTLSSKPPEHTDSHTTYTFDPKQPVPTIGGNIS
                     SNQGLISNGGYDQRSRDDTHGAKDRLPLSERRDVLVYRSSPLDADLEVTGTVEVKLWV
                     GSTAPDTDFTAKLIDEIPPNPDYPLGFDLNLGDSILRARYREGLDHQASPLDPSDVVP
                     ITITLYPTANVFKKGHRIRLDISSSNYPRFDVNPNTSDPLGDYRRMVPADNTVFHEAG
                     RPSQLVLPVVAQSR"
     gene            3758..4228
                     /locus_tag="VHS97_12470"
     CDS             3758..4228
                     /locus_tag="VHS97_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449063.1"
                     /translation="MIKRWFMILAAGTLSTTLMGCGNPPGLYPVYGSVFHKGEPAAGA
                     VVYFHREGPAAGSPQTIPFGIVEDDGHFNLTCDNLGNGCPPGKYAVLVEWKDGSGDGV
                     VPVNTKGKTKLVKRSRVRSGPDRLSGRYFDINKPLLHAEVLPQSNPLPAFEVGG"
     gene            4295..>5276
                     /locus_tag="VHS97_12475"
     CDS             4295..>5276
                     /locus_tag="VHS97_12475"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449064.1"
                     /translation="MALVQTNTTRKTGFTLIELLVVIAIIAILISLLLPAVQSAREAA
                     RRAQCINNLKQIGLGTLNFESTFGTLPPKGQVLSTPDLNADAQAAIPSISASYLTLIL
                     PYMEQSNIYNQINLFQAASNTANIPPTTGTGALHSGTNSVYSVAINSFLCPSSPGPAT
                     INYFNTFWGPYGDGGGDVCTPGAPAPPSSVSNLNPPPVQIWGRTDYFPIPGLHNTALQ
                     AAGMSPAYIAAVGEGKDSGTITGPQITGTMRLASITDGTSNTMMVSECGSKPIGYNGF
                     RQIYKSEVNGLPVDGIIEPVSSAGGAWADNFTYSSLAGAQGRQNGIRGGTC"
BASE COUNT         1046 a         1601 c         1581 g         1048 t
ORIGIN      
        1 tgacgagcac gtcgcacggc tcgaatcggc gatcgacacg ctcgaaaccg agttgaagcc
       61 tttgaaccag ttcattttgc cgggcggcac gctcgcggca gcgcagatcc atcttgcgcg
      121 aacgatttgc cgccgtgccg agagattgac cgtcaagcta tcgcgccagc tgggggagga
      181 cgttcccagt acgttgatcg tctacttgaa tcgcctgagc gactttctgt tcgtcctcgc
      241 ccgcgctgtc aatcaccgcg cggacgtgcc cgatattccc tggaaggggc tgtgaaatgg
      301 cgaccagtgc agagcggaca atggcccagt cattacaaga caaggttgtg ctgatcaccg
      361 ggggccggag agtcggaggc gagctggcgc tgttgatggc ggctcagggt tggcgcgttg
      421 cgatgacgta tcacaccagc cgcgcggcga tcgagcgcat aatcaagtct gtcgaggaag
      481 aggggacaga ggggctggcg atcgccgccg atctggcgcg accggaacag gcccagaatg
      541 ccgttaatca agtcgtcgcg cggtttggcc ggctcgatgc cctggtgaac atggcgagcg
      601 tgttccgccg cacgccgctg gccgatcttt ccgcgcgcga ctttgacgac atgatcgccg
      661 gcaacctggc cgccccatat cacacggccc tctccgcggc ccgccagatg ctgaaacagc
      721 ctgaaatcga tggtatcaag gggaagattg tcgtcatcgg cgattgggcg accgatcgcc
      781 cgtacaagga ttatctcccc tatctcgtcg ccaagggggg gcttaaaacg atgaccctgg
      841 cactagccaa ggaactcgcc cctcacatca ctgtcgccat gattcagccg gcgatgatcg
      901 atccgccccc tcaattgacg gacgatgaca aagcggccgt cgttgcagcg acgccgctac
      961 gccgcgtcgg ctcgccggac gacgtgaatc gcctcatcct ctacttgctc gaagggacga
     1021 actttgtgac tggcgcgtgc ttccgcgtcg acggcggtcg gttcttgggg gaggactcgt
     1081 gctgacgcct tactcacaat ttcatatata aattagccga ttggcttatg gaccgagtct
     1141 ggcgatgaca aactcaacgg gattgcgcca cgacgggcaa gaccaattgc gacggacggc
     1201 cggcctcgtg gaagaccgtg ttgtcggccg ggaccatgcg ccggtagtcc cccaaggggt
     1261 cgctggtgtt gggattgacg tcgaagcgcg ggtaattgct gctcgagata tcgagtcgaa
     1321 tgcgatggcc tttcttgaag acattggcgg tcgggtaaag cgtgatcgtg atcggaacca
     1381 cgtcgctcgg gtcgagcgga gaagcttggt ggtcaagccc ctcacgatat cgagcgcgca
     1441 agatcgagtc gccgagattc aggtcgaacc cgagaggata atccggattc ggtggaatct
     1501 cgtcgatcag cttggcggtg aagtcggtat cgggcgcggt cgagccgacc cacaatttca
     1561 cttcgaccgt ccctgttact tccaggtccg cgtcaagcgg ggaacttcga tagacgagca
     1621 cgtcgcggcg ctcggatagg ggtagccggt ctttggcccc gtgagtgtca tcacgagacc
     1681 tctgatcgta cccgccattc gagatcagcc cttgattcga tgaaatgttg ccgccgattg
     1741 taggcaccgg ctgtttggga tcgaacgtgt acgtggtatg gctatctgta tgctctggcg
     1801 gcttgctcga gagtgttcca tcggcgttca agtacaggga agtcggcgcg actcgcgccg
     1861 ggggccactc gcgctcggcg cgccagaaac cgccgtgctg gagccggccg gccggtgagc
     1921 gccggtcgtc accggtgccc atgatgtaga gcaacacagg tgcgtcatcg tcgactccgt
     1981 tcttgtctcc cttcaaccac cgatcgtacc accgcagccg gaaagcgagc aggtcgatgc
     2041 cggcctcttt tgtgaactcg acttcgccgg cggtgtaggc ggtttggccg ccgtgaaccc
     2101 agggaccgat gaccagccgc tgtggtgagc tcttggcctt ggagagtgat tgatagttca
     2161 tcgtgacctg acgcgtccat gagtcgtacc agccggtcat gtgcaataca ggtacatcag
     2221 catagtcgtt caaatgatcg actaccgaca ttcccttctt gtgccaaaac ggagattcgg
     2281 gcccgctccg catcgcctcg accagccatg cctcgtactc ggggaccacc ttgagtggag
     2341 tggtgcccat gcgcaaaggc aggttgtcga cgtgctgccg gatccgcaaa ccgttctcga
     2401 tcaaggtgcg ccgcagggcc gggtcagcca gcgccgcctt gctgttgggc gctccagtct
     2461 ggaagatcca gttcatgaaa cgcagctcga aagcaccccc atgcctcatc ccgctgaccg
     2521 cgcaattcga cacggcgtcg atgggaacca tcgtcgtcaa gtggggcggt ttcatctctg
     2581 caagcgcgtg ctgagtgccg ccgggatagc tggtgccaaa tgtcccgacc ttgccgtcgg
     2641 accagtcctg acgggcgatc cactcgacga tctcatatcc gtcctgggga tcgtcgtcga
     2701 tcaatcgcca ggtcccctcg ctggcataac ggcctcggac gtcgtttgcg acgacgacat
     2761 agccacgctc ggcgtagtag cggccctcgg ctccggcgcc gtccttgttg tagggcgtgc
     2821 gcgtcaagag cgtgggaaac cgcgctggag ccacgttgcc gtcgcgggcc ggccggtaga
     2881 tgtccgcggc gagatggacc ccgtcgcgca tcgagaccat cacattcttc tcaacaacga
     2941 ctccaaactc tggcgcccta gtaccgaccc gcggagactg ggcgcggacg gctcgaatac
     3001 tcggttcaat cagcggcagg aacccggcaa tcaccagcgg tataacggat agcggccttc
     3061 gtcgcgacat ggttgcgttc cttggaaatt atgattccat cgcatggtcg atcacctccc
     3121 accgcatggt aacgattgtc gtccgcggat catggaaagc aactggaaag cgggaatcat
     3181 tgactccagc tcggaaattc cgcgactggc ggatcgattt tccttgacga atcgtgctgc
     3241 cgatcgatag cattcgcaag acgtccaaca ggactacgtt ttcattcgag gattcgatgc
     3301 ttgggcttgt atacgtcaat tcgcactctg agcacgacct cttcatccgc ctcttcatga
     3361 ccagcggcgg cggctggtag ttcccgggca ggtcgccggc catcggcgac cagggcatgt
     3421 caacgtgccc accgagcacg atcgattcag cgccccccac ttgcgagcgg cggcgcctag
     3481 aacggcaacg atctcaacac ttccacgaca gcttgtggaa gtgagctatc gcgccgctgc
     3541 gaaatacagc cggatcctgg cgatcggctc gccttccgat tgatgccgca gtccagcaca
     3601 gcgcaaggca ttattccgct ttcacgtaca caaatccgga cagtgtccgg tacgtccgat
     3661 gcacgtatgt tatcccgatc cttttaggat ccagcaccgc cgcggtcacg accgtggtcc
     3721 ggcgaaggtc ggaaatctgg aaatggagtg ttcaataatg atcaagcgct ggttcatgat
     3781 cctggccgcc gggaccttgt ccactaccct gatggggtgt ggaaacccac ccggacttta
     3841 ccctgtttac ggctcggtat ttcacaaggg tgagccagca gcgggagcgg ttgtttactt
     3901 ccatcgcgag ggtcccgccg ccgggtcgcc gcagacgatt ccgttcggaa tcgtggagga
     3961 tgacggtcac tttaacctga cttgcgacaa cctgggaaat ggctgccccc ctggcaaata
     4021 tgccgtcctc gtcgagtgga aggacggatc gggcgacggc gtggttccgg tgaacactaa
     4081 gggtaagacc aagctggtga aaagaagtcg cgtccggtcg gggccggacc ggctcagcgg
     4141 ccggtatttc gacatcaaca agcccttgct ccatgccgag gtcttgcccc agtccaatcc
     4201 tctgccggcc ttcgaggtcg gtggttgatt aacggatgcc gtcagtttcg acggcgtcac
     4261 attaatgaga aattttttct ttggagatga gaacatggct ttagtccaga caaacacgac
     4321 gagaaagacc ggtttcacac tgatcgaatt gctggtggtc atcgcgatca tcgcgatctt
     4381 gatctcgctc ctcttgcctg ccgtgcagtc ggcgcgcgag gcggcgcgcc gggctcagtg
     4441 catcaacaac ctcaaacaga tcggcctggg tacgctcaac ttcgagagca ccttcgggac
     4501 cctgcccccc aagggccagg ttctctcaac accggatctg aacgccgacg cccaggccgc
     4561 tataccgtcg atctcggcgt cgtacctcac cctgatcctg ccttacatgg agcagtcgaa
     4621 catctacaac cagatcaacc tgttccaggc cgcttcaaac acggccaaca tccccccgac
     4681 cacaggaacg ggggcgttgc actcgggcac gaactcggtg tactcggtcg cgatcaacag
     4741 tttcctctgc ccgtcatcgc cgggcccggc gaccatcaac tacttcaaca cattttgggg
     4801 gccgtatgga gacggcggcg gcgatgtttg cacacccggt gcaccggctc cgcccagcag
     4861 tgtctcgaat ctcaatccgc cccccgttca gatctgggga cggacggatt atttcccgat
     4921 ccctggtctg cacaacacgg cccttcaggc cgcgggaatg agccccgcat acattgccgc
     4981 ggtgggtgaa gggaaagact caggcaccat taccggcccg cagatcacgg gcacgatgcg
     5041 gctcgcctcg atcaccgacg ggacgtcgaa cacgatgatg gtctcggaat gcggatcaaa
     5101 gccgatcgga tacaacggat tcaggcagat ttacaaatcg gaggtaaatg gcctacccgt
     5161 cgacgggatc atcgaaccag tcagcagcgc cggtggggca tgggccgata acttcaccta
     5221 ctccagcctg gctggtgccc agggacgaca gaacggcatt cgaggcggca cgtgca
//
LOCUS       DATAQL010000717         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_685173, whole genome shotgun sequence.
ACCESSION   DATAQL010000717 DATAQL010000000
VERSION     DATAQL010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685173"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VHS97_12480"
     CDS             <1..1456
                     /locus_tag="VHS97_12480"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3449065.1"
                     /translation="SVKGLAPLYRLVLTGTPMENRIDELASIVEWVDDKALEPKWRLN
                     SLHATRADGRREVVGARNLDTIRDRLRHCMVRRVRQDVLDQLPSRTDTRVPIEMTEDQ
                     MIAHEDLNQPIAQLVARSLKRPLTQAEFLKLMQLLTTQRIISNGMVQLNFEAMWPTVR
                     GRAPEESVLKSLSAPKLIELRQLVRQIVLEQGRKVVVFSQWRRMLTLAQWAVSDVLRE
                     GGLRSGFFTGAEGQRRRTQNIVEFHDDPDFRILFTSDAGGVGLNLQRAANCVVNLELP
                     WNPAVLEQRIGRIYRLGQKLPIDVYNLVCEQGIESRIAALVGSKQAFFKGLFDGDSDT
                     VDFGQSSNFLAKVQKLYEPAALEAVASTGNVADEDADGAETGLDDEVQDPFAELIEAG
                     DESRDQAIAPTANGDAAAVPFEPVSSTALEQHSAVAAMPASLEVRDFFSKLAVRREPS
                     GKVVIEAPAEAASNLAALFEGMAALLSAVSQPKA"
     gene            1609..2955
                     /locus_tag="VHS97_12485"
     CDS             1609..2955
                     /locus_tag="VHS97_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3449066.1"
                     /translation="MDRRQFFKHTAAAGAGTLLAGGAPAFPRSLAQSPNEKVRFACIG
                     LGGKGDSDTNDAGEHGEIVALCDIDSEVLDKMGKKYPKAKKYFDYRKMLEELGDKIDA
                     VTVSTPDHTHAPASVMAMRMGKHCFCQKPLTWSVEEARLMRTLAAEKKLCTQMGNQGT
                     AENGFREGVELIRSGILGPVKDIYVWTDRPVWPQGISRPKGMPGVPNKLRWYEFLGAA
                     PDRPYHPAYQPFNWRGWLDFGTGALGDMACHTINIAAMALELFDPESVEVVDTSGIVD
                     HESYPVWSIIRTRFGQRNGRGPLNLTWFDGGNKLPDEKRSYKEHLQGENAPDSGLLIV
                     GEQGTFFSMNDYGAEHTLLPKDKFKEVTKPNATLPRSPGHFTEWVDAIKSGDPTKAMS
                     NFAYAGRLTETVLLGVVALKAGTAIEWDPVAMKAKNVPSADQYIRRDYRKGFSIHA"
     gene            complement(3058..>3590)
                     /locus_tag="VHS97_12490"
     CDS             complement(3058..>3590)
                     /locus_tag="VHS97_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449067.1"
                     /translation="SNISRRFASITDGLSNTLLASEVKTYMPSYHDCGNIPPPGPTGA
                     SAYPDIATVLASVAAAPTSGCKPVTGPSGMPGGGHTHWCNGNSFYDGFTTALPPNTVS
                     PAGSPAIDSDMSSEDEDDGGPTYSAVTSRSYHAGGVNALFADGSVHFVKTSINVQTWR
                     ALGTVGSGEVISSDSY"
BASE COUNT          710 a         1068 c         1141 g          671 t
ORIGIN      
        1 atcggtcaag ggtctggcgc cgttgtatcg cctggtgttg accggaacgc ccatggaaaa
       61 ccggatcgac gagctcgcgt cgatcgtgga atgggtcgat gacaaggcgc tcgagcccaa
      121 gtggcgtctg aactcgctgc atgcaacccg cgccgatggc cgccgcgagg ttgtgggcgc
      181 tcggaatctg gacacgatcc gcgaccgcct gcggcactgc atggttcggc gtgttcgcca
      241 agacgtgctc gatcagctgc cgtcccgcac cgatacccgg gtgccgatcg agatgaccga
      301 agatcagatg attgcgcatg aagaccttaa tcaaccgatt gcgcagcttg tggcgcggtc
      361 gctcaagcgc ccgttgaccc aggccgagtt tctcaaattg atgcagttgc tgacgaccca
      421 acggatcatc tccaacggca tggttcagct caatttcgag gcgatgtggc cgacagtccg
      481 cggtcgcgcc cccgaggaaa gcgtgctcaa gagtctctcg gcacccaagc tgatcgagct
      541 gcgacaactc gtgcggcaaa tcgtgctcga gcagggccga aaggttgtcg tcttcagcca
      601 atggcggcgc atgctgaccc tggcccagtg ggccgtcagc gacgtgcttc gtgagggtgg
      661 tctgcgttcg ggctttttca ccggcgcgga gggacaacga aggcgcacac agaatatcgt
      721 cgagtttcat gacgaccccg actttcgcat tctcttcacc agcgatgccg gcggcgttgg
      781 cttaaacctt cagcgcgcgg ccaactgcgt ggtcaatctc gagctcccct ggaacccggc
      841 cgtgctcgaa cagcggatcg gccgcattta ccgtctgggc cagaagctgc cgatcgatgt
      901 ctacaatctc gtctgcgagc agggcatcga atcgcggatc gccgctttgg tcggctcgaa
      961 gcaggccttt ttcaaaggac ttttcgacgg cgacagcgac acggtcgact ttggccagtc
     1021 ttccaatttt ctcgccaagg ttcaaaagct ctacgagccg gccgcgctcg aggccgtcgc
     1081 atcgaccggc aacgtggcag acgaggacgc cgatggcgcc gaaaccggcc tggacgacga
     1141 ggttcaagac ccgttcgccg agctgatcga agccggcgat gaatcgcggg accaggcgat
     1201 cgccccaacc gcgaacggtg atgccgccgc cgtaccgttt gagcctgttt cgtcgacggc
     1261 actggaacag cacagcgccg tagcggcgat gcccgcgtcg ctggaagtgc gcgacttttt
     1321 ctcgaagctg gcggttcgcc gcgagcctag tggcaaggtc gtgatcgagg cgcccgccga
     1381 agcggcatcc aacctggccg ccttgttcga gggaatggcg gcactcctct cggcggtttc
     1441 ccagccaaaa gcctgagcgg attctacttg tcctcacgca agggcaggtt gcggactggg
     1501 ccgtcggacg gcatgctaaa tgggatcggc aatgttgtcc gcgctcacca acgctcccct
     1561 gccccgccaa ggccctacct gatacccaat ccctcggagt acctcaccat ggatcgacgt
     1621 caattcttca agcacaccgc ggctgccggc gcgggtactt tgcttgcggg tggcgctcct
     1681 gcgtttcccc gctcacttgc acagtctccc aatgaaaaag tacgcttcgc gtgcatcgga
     1741 ctcggcggca agggcgacag cgacaccaac gacgccggcg aacacggtga gatcgtcgca
     1801 ctgtgcgaca tcgacagcga agtgctcgac aagatgggga aaaagtaccc caaagcgaag
     1861 aaatacttcg actaccgcaa gatgctcgaa gagttgggtg acaagatcga cgcggtgacg
     1921 gtcagtacgc ccgaccacac tcatgccccg gccagcgtga tggcgatgcg catgggaaag
     1981 cattgctttt gccagaagcc gttgacgtgg tcggtggagg aggcgcgcct gatgcgcacg
     2041 ctcgcggccg agaagaagct gtgcacccag atgggcaatc aagggactgc cgagaatggc
     2101 ttccgcgagg gggtcgagct gattcggtcc gggattctgg ggcccgtcaa ggacatctac
     2161 gtctggaccg atcgaccggt ctggccgcag ggcattagtc ggcccaaggg aatgccgggg
     2221 gtcccgaaca agttgcgctg gtacgagttc ttgggtgccg cccccgaccg gccttaccat
     2281 cccgcttacc aaccgtttaa ctggaggggg tggctcgact ttgggacggg tgctctgggg
     2341 gacatggcct gccacaccat caacattgcc gcgatggctc tcgagctttt cgatcccgag
     2401 tcggtcgagg tcgtcgacac gtcgggcatt gtcgaccatg aaagctatcc ggtttggtcg
     2461 atcatccgca ccaggttcgg ccagcgaaac ggacgcggcc cgctcaatct cacctggttt
     2521 gacggcggca acaagcttcc cgatgaaaaa aggtcgtaca aggaacacct ccaaggcgaa
     2581 aacgctcccg acagcgggct cctgatcgtt ggcgagcagg gcacgttttt ctccatgaac
     2641 gactatggcg cggagcatac gctgcttccc aaggacaaat tcaaggaggt caccaagccg
     2701 aacgcgacct tgccgcgctc gcccggccac ttcacggaat gggtcgacgc catcaagtcg
     2761 ggtgatccga ccaaggcgat gtccaatttc gcatacgccg gccggcttac tgaaaccgtg
     2821 ctgctgggcg ttgtcgcgct caaggcgggc acggccatcg agtgggatcc cgtggcgatg
     2881 aaggccaaga acgtgccatc ggccgatcag tatatcaggc gcgattatcg caagggcttt
     2941 tcgatccacg cctgatgggt gtctgccgtt ggggatatgg ggatcaagag gtcagaaggg
     3001 agaaaacggt tcacacgggt tttctccctc ctgacgaaga tcggctgggc cgatgcgtca
     3061 gtaagagtcc gaagagataa cctctccgct cccgaccgta cctaaggcgc gccaggtttg
     3121 aacgttgatc gaagtcttca cgaagtgcac gctgccgtcg gcgaacagcg cattaacgcc
     3181 gccagcatgg tagctgcgtg aggtcacggc ggagtaggtt ggtccaccgt catcctcgtc
     3241 ttcggagctc atatcgctgt cgatagccgg cgagcctgcc ggtgagacgg tgttgggggg
     3301 cagggccgtg gtgaaaccgt cgtaaaacga gttgccgttg caccagtgag tgtggccgcc
     3361 tcccggcatg ccggaggggc cagtgacggg cttgcatccg gatgtgggag cggccgcgac
     3421 gctggcgagc actgtggcga tgtcggggta cgcagacgcg cccgttggtc cgggaggggg
     3481 aatattgccg cagtcatgat atgaaggcat gtaggtcttg acctcggatg ccagaagtgt
     3541 gttactcaga ccgtcagtga tcgatgcgaa acggcgactg atgttcgagc
//
LOCUS       DATAQL010000718         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3126437, whole genome shotgun sequence.
ACCESSION   DATAQL010000718 DATAQL010000000
VERSION     DATAQL010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3126437"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VHS97_12495"
     CDS             complement(<1..884)
                     /locus_tag="VHS97_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX3449068.1"
                     /translation="MHESYENPLTSRYAGRQMAKLWSAQAKFSTWRRLWIALAEAQRA
                     LGLNITEEQIADLRSQTDTIDFNVADAYERRFRHDVMAHIHALGDAAPLARPIIHLGA
                     TSCYVTDNADLILMRAALEIVRDKAVAAIDALAAFAQCWKELPCLGYTHFQPAQLVTV
                     GKRASLWCHELVLDLGEIERRLKELKLLGVKGTTGTQASFLALFGGDDAKVEALDQLV
                     ARAFGFDGSYAVSGQTYSRKIDAQILATLGGIAESAHRFGTDLRLLAHERELDEPFEA
                     EQIGSSAMAYKRNPMRAER"
     gene            1164..1820
                     /locus_tag="VHS97_12500"
     CDS             1164..1820
                     /locus_tag="VHS97_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3449069.1"
                     /translation="MSESSDRVLLDLIRRGGPLTVGDMAASLGVTATAIRNRLTRLLG
                     AGLVERQAEHQDRGRPKHTYRASVEAQKRLGQNYTDLALVLWDEMMSTVSDRKLRRLL
                     FTRVTDRLAEMYRSQVTGAEWEGRLVQLSNLLHDRGVEAEVAREAGEAIPILRQHSCP
                     YYELAEADRAICALERKMFEKVLGRSLRLSQCRLDGDRFCDFQAKPIPITLPETAQVG
                     "
BASE COUNT          404 a          583 c          626 g          417 t
ORIGIN      
        1 cgctcggcgc gcatgggatt gcgcttgtaa gccatcgcgg atgagccaat ctgttcggcc
       61 tcgaacggct catccagttc gcgttcgtga gccagcagcc tcaggtccgt accgaaacga
      121 tgagcgcttt cggcgattcc gcccagtgtg gccagaatct gagcgtcgat tttccgggag
      181 taagtctggc cagaaacggc gtacgacccg tcgaagccaa acgctcgggc gaccagctgg
      241 tccagggctt ccaccttggc gtcatcgccg ccaaagaggg ctaggaaact tgcctgcgtc
      301 cccgtcgtcc cttttactcc gagcagtttc agctccttca gcctgcgctc gatctcgcca
      361 agatcgagaa ccagttcgtg gcaccacagc gaggcccgct tccccactgt aaccagttgg
      421 gcaggctgga aatgggtgta acccaggcag ggcagttcct tccagcactg ggcaaaagca
      481 gccagagcgt cgatcgcggc gacggctttg tcgcggacaa tctccaaagc cgcgcgcatc
      541 aggatcaaat cggcgttatc cgtgacgtaa caacttgtcg ctcccaggtg aatgatgggg
      601 cgtgccaggg gagcggcatc gcccagggcg tgaatgtgcg ccatcacgtc atggcgaaac
      661 cgccgttcgt aggcatcggc cacgttgaag tcgattgtgt ccgtctgtga gcgcaggtcg
      721 gcgatctgct cctcggtaat gttcaatcct aaagcgcgct gggcctcggc cagggcgatc
      781 cacagacgcc tccaggtcga aaacttggcc tgtgcggacc atagcttcgc catctgccgt
      841 ccggcgtacc ggctggtcaa gggattttca taggattcgt gcatcagcgt cgcctcgcaa
      901 acgagcagtg aatctcaatc gtagcgcctc cagtccccga ccgcaaggtt ccgggggccc
      961 acttgacgca aaaccacact gcgaatacgt ttccgtctct aacatcctat ttttcacaat
     1021 ttttcgtgtg aaaaaagcga ataaggagcc tccgactgat ggacgacccg gcctcgggct
     1081 cctagaatga gttgatcgcg agtgatgttg gggtcgtgtt tctcggtttc ttccgagccg
     1141 ctttgcggga cggggtgtct tttatgtctg agtcttcgga tcgagtgctc ttggacttga
     1201 tccgtcgggg cggcccgttg acggtgggcg atatggctgc gagcctggga gtcacggcca
     1261 ccgcgatacg taaccgtctg actcggctgc tcggggccgg tctggttgaa cgtcaggccg
     1321 agcatcaaga ccgcggccgt cccaaacaca cctatcgtgc cagtgtcgaa gcgcaaaagc
     1381 ggcttggtca gaactacacg gatctggctt tggtgctttg ggacgagatg atgagcaccg
     1441 tgtcggaccg aaagctgcgg cggctgctgt ttacgcgggt gaccgaccgt ctcgccgaga
     1501 tgtataggtc tcaagtgacc ggagcggagt gggaaggacg gctggtccaa ctcagcaact
     1561 tgctgcacga ccgcggtgtc gaggccgaag tggccaggga agccggtgag gcaataccca
     1621 tcctgcggca gcactcgtgt ccctactatg agctcgccga ggctgaccgc gcgatttgcg
     1681 ccctcgagcg aaagatgttt gagaaggtgc tcggaaggag ccttcggctc agccagtgcc
     1741 ggcttgatgg cgatcgcttt tgtgatttcc aggccaagcc gataccgatc acccttcctg
     1801 aaacggcaca ggttggctga ccaactcatt gggaagtggt ggcggccgaa agcccctttg
     1861 cgagaggccg ctggatggtg agggctcaaa tccgctcgat ggttcaaaca actgggtgtc
     1921 gaaagagtga cagactgatg agcaaggttc tcaagatcga aaatcttcat gtctccatag
     1981 cgggcaaaga gatcctgcgc ggggtcgact tgacgctccg ccaaggggac
//
LOCUS       DATAQL010000719         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4347002, whole genome shotgun sequence.
ACCESSION   DATAQL010000719 DATAQL010000000
VERSION     DATAQL010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4347002"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VHS97_12505"
     CDS             complement(<1..886)
                     /locus_tag="VHS97_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX3449070.1"
                     /translation="MPATPPRSDRPDIPIGHFLHYLMAECGVSTHTLAAYRSDLVHFA
                     RWRKSEAPGPLVTLNVQSLSGYVDALARAGLAPSSVCRHLASLSTFFRFMVVEGRLPE
                     NVAKLLIAPAVWDRLPTVLGPAAVERLLHAPSSQTRLGRRDRAALETLYATGCRASEV
                     VGLRPIDLDLKAGVARCVGKGNKERRVPLGSHAIRALAVYIEKDRPTLVARRPETAEL
                     FASKSGRPLSRIALWQIVKRHAKAAGLHADVSPHTLRHSFATHLLAGGADLRVVQEML
                     GHASIGTTQIYTRVELSHL"
     gene            1114..1698
                     /gene="pyrE"
                     /locus_tag="VHS97_12510"
     CDS             1114..1698
                     /gene="pyrE"
                     /locus_tag="VHS97_12510"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX3449071.1"
                     /translation="MGIGVARYKREQLIGLLQRDALKRGTFTLASGRTSHYYVDGRKV
                     TLSAEGAMLVGAGVIEQLASRPEINAVGGLIIGADPIVGATLALAAEAGLSHLRGFLV
                     RKETKTHGTGNLIEGPLEPGSTVAIVDDVATTGGSSIQAALAVEAIGCKVAVVIVVLD
                     RLEGAAAAFAARGLPFHPLLTIRDLGVEPLSATG"
     gene            1715..2458
                     /locus_tag="VHS97_12515"
     CDS             1715..2458
                     /locus_tag="VHS97_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449072.1"
                     /translation="METSLHRTLKERYAAGGSGRPEIVIGGFRVDAVDQAGRLIEVQS
                     GALGPLRGKLRLLLPEHRIRIIKPVVLKRRLIRKSTPTGPVISTRLSPKRGRLFDVFD
                     DLIGLVRVFPHTNLEIDVVGVTIEEVRLQRRRWPGYKVADRYLGETHETQSVVTALDL
                     WALLPPGCEGREPFTTYELAQMLDRPLSFAQRVAYCLRLTGAARVVGKRGNRLIYIRE
                     PENLYGFSSDSSNSSRSIRAAVVDVSESA"
     gene            complement(2380..3840)
                     /locus_tag="VHS97_12520"
     CDS             complement(2380..3840)
                     /locus_tag="VHS97_12520"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEX3449073.1"
                     /translation="MPVSVRRLPIKLEPDPTRVITRYFCPGDLKRAREIIERVLTFST
                     EEIDKNLAALEHSFATSHPDLSEIFEEHFQQVHAAIPVESKLTQTQRSFIGACFTMEY
                     AIESVALFNPSIVPALIQEGVPAGGIRFVMSLRAIGEGHLSSIVFRTGVIDAAGDIRL
                     EAPPASTKALKAILADHFHKSIFERDLAALGIAEEQFRPILEPLPALFTREQLSQAIE
                     AVRQNQETSGFLEATADSLIALTRVNYQLRLEQLLINSQSEIVIFPFSDIERHGIEDL
                     RMVRFTEDDGSHISYGTFTAFNGERIFPQLMEFRGKNTIEISLITGDSARNKGMALFP
                     RRVHGKYAMISRIDNENLYFMESDDIRYWDRASQIQAPKYPWQVIQIGDCGSPLETEA
                     GWLLLTHGVGPMRQYCIGAMLLDHDDPCRVIGQTREPLIVPVDQERSGYVPNVVYSCG
                     ALLHNRKLIIPYALSDTSTTAARIDLDELLESLEKP"
BASE COUNT          795 a         1166 c         1277 g          755 t
ORIGIN      
        1 gaaggtggct tagctcgaca cgtgtataga tttgggttgt cccaatcgac gcgtgcccca
       61 acatctcctg gacgacgcgc agatccgcgc cgccggcaag cagatgagtg gcaaagctgt
      121 gcctcagcgt gtgcgggctg acgtccgcgt gcaagccggc ggccttggcg tggcgtttga
      181 cgatttgcca aagcgcgatg cgcgataagg gccttccaga tttggaggcg aagagttccg
      241 cggtttcagg cctgcgcgcc accaaagtgg gtcggtcttt ttcgatgtag acggccagtg
      301 cgcgaatggc gtgcgaaccc aaggggactc gccgttcctt gttacctttg ccgacgcacc
      361 gcgccacgcc ggccttcagg tccagatcga tgggccgcag cccgacgacc tccgaggcac
      421 ggcagccggt cgcgtacaag gtctcgaggg cagcacggtc gcgccgtccg agccgcgttt
      481 ggctgctcgg tgcgtgaagc aagcgctcga ccgccgccgg cccaaggacc gtcggtagac
      541 gatcccacac ggcgggcgca atcagcaatt tggccacgtt ttccggcagc ctgccctcca
      601 cgaccatgaa gcggaagaag gtcgagaggc tggccaggtg acggcaaacg ctcgacggcg
      661 ccaggccagc gcgggcgagg gcgtcgacgt acccgctcaa cgactgcaca ttcagggtga
      721 caagagggcc cggtgcctca gactttcgcc agcgggcgaa atgcaccaga tccgaccggt
      781 atgccgccaa agtgtgagtt gagaccccac attcggccat cagataatgc agaaaatgcc
      841 cgatcggaat gtcgggacga tcagatcggg gcggcgttgc aggcatgaaa agagttcgcc
      901 gagcttggtc gaatcacaag gctccatgtc aagcatcggc gaatcgaggc ggtgggcttg
      961 agacgagacg gtcttggacc gccggaccgg ctctggtagg gtgtgggcgc cgccggaaag
     1021 cccacgtatt gccgccgagt ggtgcaatcg acgctgcaag gtttgcccga atgcgagcct
     1081 gagtttggac cgaaagaaac ggggtaagtg cagatgggga taggcgttgc ccgctacaag
     1141 cgcgagcagt taattggtct tctccagcgc gatgcgctca agcgagggac attcacgctg
     1201 gccagtggcc gtacctcgca ttattacgtg gatggccgca aggtcacgct ctcggccgaa
     1261 ggcgcgatgc tggtgggagc gggcgtcatt gagcagctcg cgagccggcc ggagatcaac
     1321 gccgtcggtg gactgatcat cggggccgac ccaatcgtcg gggcgacgct cgctctggca
     1381 gcggaggctg gactgagcca cttgcgcggc tttttggttc ggaaggaaac caagacacac
     1441 ggcaccggca acctgatcga gggaccgctg gagccggggt cgacggtggc tatcgtcgac
     1501 gatgtcgcca ccaccggcgg ttcatcgatt caggccgcac tcgccgtcga agccattggc
     1561 tgcaaggttg ccgtcgtgat tgttgtcctc gaccgtctcg aaggagctgc agcagccttc
     1621 gcagcccgcg gacttccctt ccatccgctc ttgacgatac gcgatctcgg tgtcgagccg
     1681 ctttcggcca cgggctaaac tctgggggca agtcatggag acgagcctac atcgcacgct
     1741 taaggaacgg tacgcggccg gtggaagtgg ccgccccgag atcgtcatcg gcgggtttcg
     1801 cgtcgatgct gtcgaccaag ccggccgact gatcgaagtc cagtcaggag ccttgggccc
     1861 gctacgcggc aagctacggc tgttgctgcc agagcatcgc atccggatca tcaagccggt
     1921 cgtgttgaag cggcggctga ttcgcaaatc aacgcccacc gggccggtca tctcgacgcg
     1981 gctaagcccc aagcgcggca ggctattcga cgtgttcgat gacctgatcg gcttggtccg
     2041 agttttcccc catacgaacc tcgagatcga cgtcgtgggc gtcacgatcg aggaagtgcg
     2101 cttgcagcgc cgccgctggc cgggctacaa ggttgccgat cgctacctgg gcgaaaccca
     2161 cgagacgcaa tcggttgtca ccgcccttga tctgtgggcc ctgttgccac caggatgtga
     2221 aggccgggaa ccattcacga catacgaact tgcccaaatg ctcgatcggc cgctctcgtt
     2281 cgcccagcgg gtcgcgtact gcttgcgact cacaggcgcc gcccgcgtcg tgggcaagcg
     2341 aggaaaccgt ctgatttaca tccgggaacc ggaaaacctt tatggcttct cgagcgactc
     2401 cagcaactcg tcgaggtcga tacgagccgc ggtggttgac gtgtcggaga gtgcgtaagg
     2461 gatgatcagc ttgcggttgt gcaacagcgc cccgcacgaa taaacgacgt tgggaacgta
     2521 cccggatcgt tcctggtcga cgggcacgat aagtggctcg cgcgtctggc cgatgacacg
     2581 gcaaggatca tcgtggtcga gcagcatcgc gccgatgcag tactgacgca tcggtccgac
     2641 tccgtgggta agcaacagcc agcccgcctc ggtttcgagc ggcgacccgc aatcaccgat
     2701 ctgtatgacc tgccagggat acttgggtgc ctggatctga cttgctcggt cccagtaacg
     2761 aatgtcgtcg gattccatga agtaaaggtt ttcattgtcg atcctcgaaa tcatggcgta
     2821 cttgccgtgc acccgccgcg ggaacagcgc cataccctta ttccgggcgc tgtcgccggt
     2881 aatcaggcta atctcgatcg tattcttgcc ccggaactcc atgagctggg gaaagatacg
     2941 ctcgccgtta aaggccgtga acgtcccgta gctgatgtgc gagccgtcat cttcggtgaa
     3001 ccggaccatg cgcaggtctt cgataccatg ccgttcgatg tcggagaagg gaaagatcac
     3061 gatttcggac tgcgagttga tcagaagttg ctccaagcgg agttgatagt tcacacgggt
     3121 aagagcgatc aagctatcgg cggttgcctc cagaaaaccc gacgtctctt gattctggcg
     3181 aaccgcctcg atcgcttgcg aaagctgttc gcgcgtgaac agggctggca gtggctcgag
     3241 gattggtcga aattgttctt cggcaatccc aagcgccgcc aaatcgcgct cgaagatcga
     3301 cttatggaag tggtcggcca gaatggcttt gagtgccttt gtgctggcgg gaggcgcctc
     3361 gagccggata tcgcctgctg cgtcaattac gcccgtgcgg aaaacgatcg acgagagatg
     3421 gccctcgcct atggcacgca ggctcatgac gaatcgaatt cccccagcgg gcactccttc
     3481 ctggatcagg gcggggacga tcgagggatt gaacaaggcg accgactcga tggcgtactc
     3541 catcgtgaag caggccccga tgaacgaccg ctgagtctgg gtcagcttgg attcgaccgg
     3601 aatggcggca tggacctgct ggaaatgctc ctcgaagatt tcgctcagat cgggatggct
     3661 cgtagcgaag ctgtgctcga gagcggccag gttcttgtcg atttcctcgg tggagaaagt
     3721 caggacccgc tcgatgattt cgcgggctct tttcagatcg cccgggcaga aatatcgcgt
     3781 gatgacgcgc gtggggtcgg gctcaagttt gatcggtaat cggcggactg ataccggcat
     3841 tttgcactcc aggacaagcc gatcaacgaa ctgagcgagt ctcgtcaaag cgatcacgca
     3901 acagccacca gagtaacccg gcaacacagg cgccgtgatc gatctggccg ctcttgatgt
     3961 gcttcgggat atcatgaacg ccaacgagct cga
//
LOCUS       DATAQL010000720         6022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2211614, whole genome shotgun sequence.
ACCESSION   DATAQL010000720 DATAQL010000000
VERSION     DATAQL010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211614"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VHS97_12525"
     CDS             <1..786
                     /locus_tag="VHS97_12525"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX3449074.1"
                     /translation="SKVRVVESDEDILLYDLAHIPGAVRIDWQGDLQDQLIRDYINAD
                     KFSALCSKAGIASDTTVIFYGDKSNWWACYAFWAFKLFGHKDCRILNGGRKLWLDQGR
                     PTTTEIPTYPPTKYVVAGSDEDRIRTFRDAVAKHAKAGLPLIDVRSPKEFTGEMLHME
                     AYPQEGALRGGHIPGAKNVPWSRAVNDDGTFKTVAELKAIYEQEIGLKPSDPVVAYCR
                     IGERSSLTWFVLTYLLGYSNVKNYDGSWTEWGNLVRAPIAKGA"
     gene            833..1276
                     /locus_tag="VHS97_12530"
     CDS             833..1276
                     /locus_tag="VHS97_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX3449075.1"
                     /translation="MPTALDTIMAELSDLDRQERIDMLLDFAKSLPPLPDRLVAHKDA
                     SHRVEECQSPVFLFVEFDGDRVSLYADAPIEAPTVRGFVSLLLEGLNGATVEEVLEVP
                     GNLVERCGLGEILGMLRVRGLTGVLRRLKAEVTRAAIDRHRAKIG"
     gene            1415..1729
                     /locus_tag="VHS97_12535"
     CDS             1415..1729
                     /locus_tag="VHS97_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX3449076.1"
                     /translation="MSSSMKVATRNELPPGGKKLVEIDGRAIAVFNVDGSYYAIDDVC
                     THDGGPLAEGELIGSEIECPRHGARFDVQTGRPLCMPAIEPVAVHAVELRGDDVYVAT
                     ND"
     gene            1829..2128
                     /locus_tag="VHS97_12540"
     CDS             1829..2128
                     /locus_tag="VHS97_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX3449077.1"
                     /translation="MIEQDALLSALRTVKDPELNVNVVDLGLVYSIQTHDDHVDVEMT
                     LTSPACPVGPEMLRSAVTALEKIEGVTKANVKLVMSPPWSPEKMSDAARDELGIF"
     gene            2656..3435
                     /locus_tag="VHS97_12545"
     CDS             2656..3435
                     /locus_tag="VHS97_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX3449078.1"
                     /translation="MRVNVKASLSRRLREVRQELFGEHGGPELARRLSLPARTWYNYE
                     TGVTVPAEVLLGFIEQTGVNPTWLLNGDGPRYRNVNHDRLLSDLTPVELIRRGLEELE
                     RSPRETVIVGPENVRAGVESEFVAIPLYPLSELADPILDPGKAEGHVLAYRSWLPNPC
                     ETIGVRLTDESMHPILPAGSIVAVDRSETDPSKLHGQIVAARPNGKPMIRWLDISGRH
                     VILRPNQNGKEFPLIPIEYDPRDSQLIIGHVVWSWSRFAHQ"
     gene            3720..5147
                     /locus_tag="VHS97_12550"
     CDS             3720..5147
                     /locus_tag="VHS97_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3449079.1"
                     /translation="MSRSCLALFEPDQLWQRAREPLFWLGPTFKLVWVNQAWENLTGY
                     PAESVVGLTCTAHGPTGAGGLVDLATSFHPPPESLTGQPAGTLAHIFPASGESIWRRI
                     DFWPIRDEDDSSIGFLGLVRSADCRPSAANSEPSQLHLDLLEIRRLQQQRYGFESLIG
                     FGPSHRRLIEQVRLAAASTAPVLVVGESGTGKRQVARTIHHNGPGRHQPLVPFDCQAL
                     PSEILERELFGTNKFPESEPGTLAPIGGTSRPRLSLGDGSSLLICEILMLPRDLQSRL
                     AASLDTPVRLLATTCLDPEIAVQNEQLRPDLYFALTTLVIRLQPLRQRREELPALAQN
                     FLDRANERGGERKKGFSPQALSALTAYDWPGNLRELARVIDYAHSQPGEPVVAIEDLP
                     SSIRGHLSDGYPSTPHANAIKPLDDLLTEVERRLIETGLKQARGNKSRAAELLGISRP
                     RLYRRIKELNLPDDGESADEPDPSR"
     gene            complement(5407..>6022)
                     /locus_tag="VHS97_12555"
     CDS             complement(5407..>6022)
                     /locus_tag="VHS97_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449080.1"
                     /translation="DVRFAVACLHERHRRLAEEIVTQTIARERTSPGPTIEVYSERTP
                     KLIRLARVAWAVSGSVGLELMVEGLPSVVLYKVNRFDLWVARHFIKSRYISLVNLLAD
                     DEVFPEYLTWRDVSDEMVRWAVRWLDDPETRERTVDSILHLRQRVAQPGASKRAATHI
                     AHWLFDHRQKAATAPASSSVATLRGPHELGLQPSESSDAKSERR"
BASE COUNT         1210 a         1780 c         1771 g         1261 t
ORIGIN      
        1 tccaaggtgc gcgtcgtcga gagcgatgag gatatcttgc tctacgacct ggcgcacatc
       61 cccggcgccg tccggatcga ctggcagggc gatctccaag accaactaat tcgcgactac
      121 atcaacgccg ataaattcag cgcgttatgc tcaaaagcag gcatcgccag cgacacaacc
      181 gtgatctttt acggggataa gtcaaactgg tgggcgtgct acgcgttttg ggccttcaag
      241 ttgtttggac acaaggattg ccggatcttg aacggcggcc gcaaactctg gctcgatcaa
      301 ggacgtccga ccaccaccga gatccccact tatcccccaa cgaaatatgt tgtggcggga
      361 agcgatgagg accgaattcg tactttccgc gatgcagttg cgaagcatgc caaggcgggt
      421 ctgccattga tcgatgttcg cagccccaag gagttcacgg gcgaaatgct gcacatggag
      481 gcgtatcctc aggaaggcgc gctccgcggc ggccacatcc cgggcgccaa aaacgtcccg
      541 tggagccgcg cagtcaacga tgatggcacg ttcaagactg tggccgagct caaagcgatc
      601 tatgaacagg aaattggctt gaaacccagc gaccccgtcg tcgcctattg ccgtatcggc
      661 gaacgctcga gcttgacttg gtttgtcttg acctacttgc tgggttactc caacgttaag
      721 aattatgacg gctcgtggac cgagtggggt aacctggtcc gtgcgccgat tgccaagggt
      781 gcttgactcc gcgcgacggg ccgtcaggcg attttgatcg accgagggat ccatgccgac
      841 cgcactggac acgatcatgg ccgaattgag tgatctcgac cgccaagagc ggatcgacat
      901 gctgctcgat ttcgccaaga gtcttcctcc tttgcccgat cgcctggttg ctcacaaaga
      961 cgcttcccat cgtgtcgagg aatgccagtc tccagtcttt ttgtttgtcg agttcgatgg
     1021 cgaccgggtt tctttgtacg ccgacgcacc gattgaagcc cccaccgtac gaggttttgt
     1081 ctcgttgctc ctcgagggat tgaatggggc gacggtcgag gaggttcttg aggtcccagg
     1141 taacctggtc gagcgatgcg ggctggggga gatcctggga atgctgcggg ttcgcggctt
     1201 gacgggagtt cttcgtcggc tcaaggcgga ggtgactcgc gccgccatcg accgccaccg
     1261 cgcaaaaatt ggataggaca agctcgacgc cagttacgaa ggtccaaccg gaactcgccg
     1321 ccgattgcaa aggtagatta agcagggtgc aaggtcgtgc tgtgatcgac tggattagcg
     1381 gggcttccag gtgttttgaa agactcaccc agtcatgtcg tcatcaatga aagttgcgac
     1441 ccgtaacgag cttcccccgg ggggcaaaaa gctcgtcgaa atcgacggcc gtgccatcgc
     1501 cgtgttcaat gtcgacggca gttactatgc gatcgacgat gtctgcaccc acgacggcgg
     1561 cccgcttgcc gagggggaac tgatcggtag cgagatcgag tgccctcgcc atggtgcccg
     1621 ctttgacgtt caaaccggcc gaccactttg catgccggcg atcgagcccg tggccgttca
     1681 cgccgtcgag ctacgcggcg acgatgtgta cgttgcgacc aacgactgac tcttgcaagt
     1741 cgagatcggt ctgtcgcgcg ttttttgcgt cgtccgatcc ggagtgtgat cgcgtaaaag
     1801 tcatccgggc cacaaggagt ctattgtcat gatcgaacag gatgctctgc tgtccgccct
     1861 ccgtactgtc aaagatcccg agttgaacgt caatgttgtc gatctgggct tggtgtattc
     1921 gatccaaacc cacgacgatc acgtcgacgt ggaaatgacg cttacatcgc cggcgtgccc
     1981 tgtgggcccg gaaatgctac gaagtgccgt gactgccttg gaaaaaatcg agggagtcac
     2041 caaggccaac gtcaagcttg tgatgtcgcc gccttggtca ccggaaaaga tgtcggacgc
     2101 cgcgcgcgac gaattgggaa tcttttgact ccaaaaccgg ttcggacgga ttgccaactc
     2161 actgagatta tccctgcgcg catcccatct gcctgagtcc tgaaggccgc ggaaagagcc
     2221 ctggtgctct ttctcaatcg ctgcctgact tagcgcccat gatctacagc ccaggggatt
     2281 tgattgccgt ttcgccaccg ggctcgtcga ttggtggagt taaaggaacg catgttgcat
     2341 gaaatcggcc gctagtcgcc ggttgtggtg ccgatccgca tcacaatgcc taagtttgct
     2401 gaactccacg tgcgccatgg tggcgccgaa tgagaattgt gctaaattgt tatcttcctg
     2461 agtatctaac cgaaaaacag cttccgcctg cagagacgcg tcaccggtca atgaagttac
     2521 tctcaccgtt gcgctattcg gagggttttc ttgattgaca gcgcgacgga gcgatgacgg
     2581 cgtcactgtc aggcgaacct tggggggttc ccgcgtgcgg gatcgagcaa agtggctcgt
     2641 aagaaaactc cgaaagtgcg agtgaacgta aaggcgtcgt tatcccgtcg gctgcgggag
     2701 gttcgccaag aactttttgg tgagcatggc ggtccagagc tggcgcgccg tctcagccta
     2761 ccggcgagaa cctggtacaa ttacgagacc ggcgtgaccg tcccggccga agttttgctt
     2821 ggattcattg aacagacggg cgtgaacccc acgtggttat tgaacggcga tggccctcga
     2881 taccgaaatg tgaatcacga tcgtctcctt tctgacttaa cgccggtcga attgatcagg
     2941 cgcggtctgg aagaactgga acgttcgccg cgagagaccg ttatcgtcgg cccggaaaac
     3001 gtccgcgctg gagtagagtc cgagttcgtg gcgatcccgc tttatcccct ttccgagctc
     3061 gccgacccaa ttttggaccc tggcaaggcc gaagggcatg tgctggcgta ccgaagttgg
     3121 cttcctaatc cctgcgaaac aatcggtgtt cgactgactg acgagtccat gcacccgatt
     3181 ttgcccgccg gctcgatcgt ggccgtcgat cgctcggaga ccgatccgag caagcttcac
     3241 ggacagatcg tggcggcgcg ccccaacggt aagccgatga ttcggtggct cgacatcagc
     3301 ggccgtcacg tgatcctgcg cccgaaccaa aacggcaagg aattcccgct gattcccatt
     3361 gagtacgacc cccgggacag tcaacttatc atcggtcatg tggtctggtc ctggagtcga
     3421 ttcgcccatc aatagcgtcc gccgtcacta aggcgcgaaa ccatcctcag cctggcgaca
     3481 cttgcacctc cctgggcaag acgatcgcac tacagccttt cgccggcgtt ccggcaatag
     3541 gtctcgcgga tcgggcgcgc gtgcgtcatg tttttttctt tttgcttgcc ttcgcgccag
     3601 tcgagagcga tcctgaggcg aggcgaccct cagtgtggcg ggccagcagg tggggcagtc
     3661 ttccttggtc agtgagcccg cgtgtcgcga tggcttcaac tgaatcgatc gatggttcca
     3721 tgtcgcgttc atgccttgcg ttgtttgagc cagatcagct ctggcagcgg gcgcgcgagc
     3781 cgctattctg gcttggcccc acgttcaaac tcgtctgggt gaatcaggcc tgggagaatt
     3841 tgaccggata tccggccgag tcggtggtgg gactgacctg cacggcccac ggcccgaccg
     3901 gcgccggtgg cctggtcgac ctggcgacga gctttcatcc gcctcccgag tcgttgacag
     3961 gccagccagc gggaacgctg gcgcacattt tccccgctag cggcgagtcg atctggcgcc
     4021 gcatcgactt ttggccgatc cgcgacgagg acgattcctc gatcggtttt ttgggcctgg
     4081 tgcgctcggc tgactgtcgg ccgagcgctg cgaactcaga gcccagccag cttcatctcg
     4141 atttgcttga aatcaggcgg ctccaacaac agcgatacgg ctttgaaagc ttgatcggct
     4201 ttggtccatc ccaccggcga ttgatcgaac aggtacgtct ggccgcggca tcgacggccc
     4261 cggtactggt cgtcggcgag tcgggtactg gtaagcgaca ggtcgctcga accatccatc
     4321 acaatggccc gggacgccac cagccgttgg tccccttcga ttgccaggca ttgccctccg
     4381 agatcctgga gcgggaattg ttcggaacca acaagtttcc cgaatccgag cccgggactt
     4441 tggcgccgat cgggggaacg tcaaggccgc gcctttcgct cggggacgga tcgagcttgc
     4501 tcatctgcga gattttgatg cttccacgcg acctgcaatc gcgactggcg gcgtcgctcg
     4561 acacacccgt gaggctgctg gcaaccactt gcctcgatcc ggagattgct gttcagaacg
     4621 agcagcttcg acccgacctc tactttgccc ttacaactct ggtgattcgg ctccaaccct
     4681 tgcgccagcg gcgcgaagaa ctgccggcct tggcccaaaa ttttctcgat cgtgccaacg
     4741 agcgcggagg ggagcgaaaa aagggcttct ctccacaggc actctctgcc ctgaccgcct
     4801 acgattggcc gggcaatttg cgagaattgg ctcgagtcat cgactacgct cattcccagc
     4861 cgggagaacc cgtggttgcc atcgaagatc taccttcatc gattcgcgga cacctgagcg
     4921 acggatatcc atccacgccc catgcaaacg ccattaaacc gctcgacgat ttattgactg
     4981 aggtcgaacg ccggctgatc gagaccggct tgaaacaagc gcgcggtaac aaatcgcgcg
     5041 ccgccgagtt gttgggcatc tcaaggccgc gactctatcg ccgtatcaaa gagctcaatc
     5101 tcccggacga cggtgaatca gccgacgagc cagacccatc gcgatgacat acgcgactcc
     5161 gcccgccaat cgaatcaacg tatctgaaat caaggaattc gagcgcctga actgttccga
     5221 gcaagccatt cacgctctac cgatgcgttt ggctgcaaga aaccgaaaag gccccgtcac
     5281 atccatgctc gggggcccct cggcaatacg ctttgtgtct cgagtcagag cgtagtcgta
     5341 cccgattagt gtggcaaggc agacggacat tcactaccag gacgagcggc ggcgcacgtc
     5401 cacccttcac cgtcgctcgg acttggcatc gctgctctcg ctgggttgta aaccgagctc
     5461 gtgaggaccg cgcagggtgg caacggatga actcgcgggc gccgtagctg ctttctggcg
     5521 atggtcgaag agccagtgag cgatgtgcgt ggcggctcgc ttcgaggcgc cgggctgggc
     5581 cacgcgttgc cggagatgca gaatcgaatc cacagtccgt tcccgtgtct ctggatcgtc
     5641 gagccacctt acagcccaac gaaccatttc gtccgagacg tcacgccatg tgagatattc
     5701 gggaaatact tcgtcgtcag ccagcaggtt caccaagctg atatatcttg acttgatgaa
     5761 atgtcgcgct acccagagat cgaagcgatt caccttgtag agcacgaccg aagggagccc
     5821 ttcgaccatc agctcaagtc cgaccgatcc cgagaccgcc cacgcaaccc gtgccagcct
     5881 gatcagcttg ggagtgcgct ctgagtagac ctcgatcgtc gggccgggcg acgtgcgttc
     5941 ccgagcgata gtttgggtga cgatctcttc ggcaagcctt cggtggcgtt cgtgcaggca
     6001 cgcgactgcg aagcgcacat cc
//
LOCUS       DATAQL010000721         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4197092, whole genome shotgun sequence.
ACCESSION   DATAQL010000721 DATAQL010000000
VERSION     DATAQL010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4197092"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VHS97_12560"
     CDS             <1..619
                     /locus_tag="VHS97_12560"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3449081.1"
                     /translation="EAFEASSVHYWASPLDGKLCAGQEVALVGGGNSAGQAVVYLGSR
                     VAKVWLLVRGPNLEASMSRYLVDRIAGLSNVEVVTQAQVSCLEGHDGMLQAIRWRHGA
                     SGEEVRRPVRHLFLFIGAEPNTNWLSGSGVALNAKGFVLTGGDVAGARRALETSRRGV
                     FAIGDVRSNSVKRVAAAVGEGAQVVATLHAFLATANREPTPKANT"
     gene            667..906
                     /locus_tag="VHS97_12565"
     CDS             667..906
                     /locus_tag="VHS97_12565"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX3449082.1"
                     /translation="MTKIIYEIVEHDGGWAYQVDGTFSEAFPSHDLAREAADRAAKEQ
                     IVPGEATDISYEDKDGRWHDEASPGGDRPETDVEG"
     gene            1220..1582
                     /locus_tag="VHS97_12570"
     CDS             1220..1582
                     /locus_tag="VHS97_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449083.1"
                     /translation="MSKLEMLTPQNSAIALIDYQPAMFQGVQSHDRLVTFNNIQVVAK
                     TAKLFKIPTVISTVAKDSFSGPFMPEVTELFPKHDIIDRTSMNAWLDAIFRKAVHDTG
                     RKKFVAPPFLRFTRSACN"
     gene            1614..>1940
                     /locus_tag="VHS97_12575"
     CDS             1614..>1940
                     /locus_tag="VHS97_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449084.1"
                     /translation="MKILMVLTSHDKLGNTGRKTGFWLEEFAAPYFTFKDAGAEIVLA
                     SPKGGQPPLDPKSNEPNFQTDLTHRFEADADAKARLAATVRLDSVKAEDFDTVFYPGG
                     HGPMWDL"
BASE COUNT          402 a          545 c          617 g          376 t
ORIGIN      
        1 ggaagctttc gaggcctcga gcgtgcatta ttgggcatcg cctttggatg ggaagttatg
       61 cgctgggcag gaggtggctc tggtaggtgg cggcaattcg gcgggccaag cggtcgtcta
      121 tctagggagt cgagtcgcaa aggtctggct gctggtgcgc gggccgaacc tagaggcgag
      181 catgtcgcgc tacctcgtcg accgtattgc aggactctcc aacgtggagg tggtgacgca
      241 agctcaggtc agttgccttg aaggccacga cggcatgctg caggcaatcc gctggcgtca
      301 cggcgcgtcc ggggaggaag tgcggcggcc ggtacgtcac ctgttcctct tcatcggagc
      361 ggagccaaac accaattggc tatccggatc cggcgtggcg ctgaacgcga agggattcgt
      421 gctgaccggc ggagacgtcg ccggcgctcg ccgcgcgctg gagaccagcc gccggggcgt
      481 cttcgccatc ggcgacgttc ggtcgaattc ggtcaagcgg gttgccgcgg cggtcggcga
      541 gggcgcgcag gtggtggcaa cgctgcatgc ctttttggcc acggctaatc gggagccgac
      601 ccccaaggcg aacacttagg gaggcccggc caacgacgag gcgaccaaca gagtcaggag
      661 ctatacatga ccaagattat ctatgagatt gtcgaacatg acggtggttg ggcttatcag
      721 gtcgacggga cgttctccga agccttccct tctcatgacc tggctcggga agcggcagat
      781 cgcgcagcga aggagcaaat cgttcctgga gaggcgacgg acatttcgta cgaagacaag
      841 gatggtcgtt ggcacgatga agcgtcgcca ggcggtgatc gacccgagac cgacgtcgag
      901 ggctgaggga actttcattg aagacccgcg gatcacggac gccccgcgct gctcgggccc
      961 gaggccagtg cgagcatcgg ggagctgcct ttgccacggg gttagaaact cgatcagtat
     1021 agacgatgcc catggttccc atcccggagc ccccaatcgc cgggctatag tggcggcggc
     1081 actatgatct atgatagttc tggatactga aaaagcgatg catgaccgga gtccgggtaa
     1141 atggtgagct gcgcgtcttg ttcctgcaac aggtgagcca aagtctcgtc gaataacatc
     1201 agcagaggga tggatcacaa tgtccaaact cgaaatgctg accccacaga atagcgcgat
     1261 cgctctcatc gattatcagc ctgcgatgtt tcagggcgtt caatcgcatg accggctcgt
     1321 tacatttaac aatattcagg tcgtggcgaa gacggcgaag ctgtttaaaa ttccgaccgt
     1381 catttccacg gtggccaagg actcgttctc cgggccgttc atgccggaag tgaccgagct
     1441 gtttccgaag cacgacatca tcgatcgcac gtcgatgaac gcttggctcg atgcgatttt
     1501 ccgtaaggcc gtgcacgaca ccggacggaa aaagttcgtc gctccgcctt ttctgcgctt
     1561 taccaggagt gcctgcaact gactgaagtg gccccaggcg aaaggaggag tttatgaaga
     1621 tcttgatggt tctgacctcg cacgacaagc tcggcaacac gggccgtaag accggcttct
     1681 ggctcgagga gttcgctgcc ccctatttca cgttcaagga cgctggcgcc gagatcgtgc
     1741 ttgcctcgcc gaagggcggc caaccgcctc tcgatccgaa aagcaacgag ccaaactttc
     1801 agaccgacct tacgcatcgg ttcgaagctg atgcagacgc aaaagcgcgg ctcgcggcaa
     1861 cggttcgcct tgacagcgtc aaggccgagg acttcgatac cgtcttctac cccggcggcc
     1921 acggccctat gtgggatctg
//
LOCUS       DATAQL010000722         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2058986, whole genome shotgun sequence.
ACCESSION   DATAQL010000722 DATAQL010000000
VERSION     DATAQL010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058986"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VHS97_12580"
     CDS             <1..358
                     /locus_tag="VHS97_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449085.1"
                     /translation="DRHLGVREIREAVSRVSSWDRDGDGQIRSDEIPHHYQLSIGRGQ
                     ITAVGMSRFASPGMMAAPGTPAPAAGPSWFRKMDRNRDGDVSRREFLGPHAAFERLDG
                     DRDGLIDAGEAAKAKP"
     gene            455..1348
                     /locus_tag="VHS97_12585"
     CDS             455..1348
                     /locus_tag="VHS97_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449086.1"
                     /translation="MTRSNQGDPPDLSLDGPCSATRRSFLAMMAALIGSSASAQDDTQ
                     DRGKSTPRLESMRRLAKEVKVCESTDGKQGPPLALRPEPLLRYTNATLGVVDGTLWGW
                     GERGRPSAVMKLGFRGPARGQRHWAYRVNVLSAKQIEVTFGDGQTWSSRKPGLELSPL
                     PDAPAPADSAARRLIQSKDIARRHSVSAENTNPRGRIQLRLLPKPIDRYSDPKVGLLD
                     GVLFSFVYTNNPSVLLVLEAWSEDKGARGWRYAFVRQGYGEVTALLDGKPVWSLSSDR
                     PPADTGLFITRQMPASPEEQD"
     gene            1478..2374
                     /locus_tag="VHS97_12590"
     CDS             1478..2374
                     /locus_tag="VHS97_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449087.1"
                     /translation="MRARAIRHDRRDLSLDEPRPATRRSFLAMMAALIGPSASAQDDA
                     EDRGKSSPRLEAMRRLAKEVKVCEISDGKPGRPLVLRPEPLLRFSNPADVTLDGTLWG
                     WGERGRPPALIKLGLRERPPGQMHWHFGVTALSPKRVEVEFGDGSRWSSRLPGLPLRS
                     VPDAPPPADSAAQRLTQAKAIARRVSIGVERQTAARRIQLRLLPRPIDLYSDPTAGLL
                     DGVLFGFVDATVPVVLLLLEAWSEGPGASKWRCAFMRQAAGEMTALLDGKLVWSVPRV
                     PQPADTELFKARGMPASPEEQD"
     gene            2645..>3691
                     /locus_tag="VHS97_12595"
     CDS             2645..>3691
                     /locus_tag="VHS97_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3449088.1"
                     /translation="MRRNSIKLTLGPADDSRGSVRTGVGLARTTFLSRERTGLIVVGI
                     MVLIWLSSNDISQDPIPMRWARGEAGGMTLGLTISPDGTTIATVDSAGRVAIWDKEDD
                     WRISRFLDYGGLAWSVAFSPDGRFLAVGGGDTAIVMFDLSTGATPRALNAPAGQTKAL
                     AFSPDGTTLAATSGRNGEIVLYDLGAGRVRTTLLGDLPAMSIAFSPDGRSLASGARDK
                     KTVKLWDLETGRSRILFRDASGPISSVAFSSDGSLLAAACSIDQLVRLWDVDSGLLWR
                     QFEGHRGGTNSVNFSPDGETLTTSGSDGFVKLWRVATGEQLASLDGQSLWLPNLAFSA
                     DGQTLFATGSDNNIR"
BASE COUNT          677 a         1144 c         1222 g          648 t
ORIGIN      
        1 cgaccggcat ctcggtgttc gcgagatccg agaggcggtt tcgcgggtgt cgtcgtggga
       61 tcgcgatggc gatggccaga tccgctctga cgagatcccc catcactacc aattgtcgat
      121 cggccggggt cagatcacag ccgtcggcat gtcccggttt gcgagccccg gaatgatggc
      181 ggcgccgggc acgccagcgc cagccgcggg tccgtcgtgg tttcgcaaga tggatcgaaa
      241 ccgcgatggc gacgtctcac gccgggaatt tctcggtccg catgccgcgt tcgagcgact
      301 tgacggcgac cgcgacggcc tgatcgatgc gggcgaggca gcgaaggcga agccatgagt
      361 gggcggagcc tgccccagtc gtcatgttga ggcatgaatg cgcctgcgac gcgagcgagc
      421 ccaaaacgag tggacgcagc acgtggaggg accgatgacg agatcgaacc aaggtgatcc
      481 accggacctc agcctcgacg ggccctgctc cgccaccagg cggtcgtttc tggcaatgat
      541 ggcggccctg atcggctcct ccgcttcagc gcaggacgac acccaggatc gcggcaaatc
      601 gaccccgcgc ctggagtcaa tgcggcgact ggcgaaggag gtcaaggtct gcgagagcac
      661 ggacgggaag cagggaccgc cgctggcgct ccgaccagag ccgctccttc gctacaccaa
      721 cgccaccttg ggagtggtcg acgggaccct gtggggctgg ggcgagcgtg gtcgaccttc
      781 cgcggtcatg aagctgggat tccgggggcc ggcgcgtggt cagaggcatt gggcataccg
      841 tgtcaacgtg ctgtcggcga agcagatcga agtaacgttc ggcgatggcc agacctggtc
      901 gtcgcggaag cccgggttgg agctgagccc gctgcccgat gcgccggcgc cggccgattc
      961 ggcggcccgt cgcctcattc aatccaagga catcgcccgg cggcactcgg tcagcgcgga
     1021 aaacaccaat ccgaggggca ggatccagct ccgattgctg cccaaaccga tcgatcggta
     1081 tagcgacccg aaggtcggtc tcctggacgg agtgctgttc agtttcgtct acacaaacaa
     1141 cccatcggtt ctcctggtgc ttgaggcctg gtccgaggac aaaggagcgc ggggctggcg
     1201 ttacgccttc gtgaggcagg gctacggcga ggtgactgca ctcctcgacg ggaaaccggt
     1261 ctggagcctg tcgtcggacc gaccgccggc tgataccggg ttgttcatca cgcgacaaat
     1321 gcccgcgagc cccgaggaac aagactgacc ggatggcgct tacgtacaag gccttaaggg
     1381 tatacggtcg gtcccgacca caaaattatt atcatgaaag aagccgaaga ctacgtcgac
     1441 cgtgagatgc aggctccctg cagtcaggag aggaaacatg agggcgagag cgatcaggca
     1501 tgatcgacgg gacctcagtc tcgacgagcc acgccccgcg accaggcggt cattcctggc
     1561 aatgatggcc gccctcatcg gcccctccgc gtcagcccag gacgacgccg aagaccgcgg
     1621 caaatcgagt ccacgcctgg aggcgatgcg gcgtttggcg aaagaggtca aggtctgcga
     1681 gatctcggac gggaagccgg gacgaccgct ggtgctccgc ccggagccgc tccttcgctt
     1741 cagcaacccc gccgatgtta cgctcgacgg gaccctgtgg ggttggggcg agcgtgggcg
     1801 gcctccggcg cttataaagc tgggactccg agagcggccg cctggtcaga tgcactggca
     1861 cttcggcgtg accgcgctgt caccgaagcg ggtcgaagtc gagttcggcg atggctcgag
     1921 atggtcgtcc cgattgcccg ggctcccact gcggtcagta cccgatgcgc cgccgccggc
     1981 cgactcggcg gcccagcgcc tcacccaggc caaggccatc gctcggcggg tgtcgatcgg
     2041 tgtggagaga caaaccgccg cccgcaggat ccagctccgg ctcctgcccc gaccgatcga
     2101 cctctatagc gacccaacgg ccggtctcct cgacggggtg cttttcggtt tcgtcgacgc
     2161 gaccgtcccg gttgttctcc tgctccttga ggcctggtcg gagggcccgg gtgcaagtaa
     2221 atggcgttgc gccttcatgc ggcaggccgc cggcgaaatg accgccctcc tcgacgggaa
     2281 actggtctgg agcgtgccgc gcgtccccca accggctgat accgagttgt ttaaggctcg
     2341 agggatgccc gcgagcccgg aggaacaaga ctgaacagat cgcgctctcg tgcgcggcac
     2401 gacacgacca tgattcacga acgccgggtg tgctcgactg tgctctcctg gcatcggccc
     2461 tgtcgaaaaa tgtgattcgc ttcaagtcga gcgggttctc cgccagccga tcgagctgcg
     2521 cgcagggcac gctccacttc cggtggaaag gtcccggcga cccctgtgct cgaagagcga
     2581 cgggacggtc gccccaagag gcttcgcgcg cacttctgac gtttgtcatg ggagacgttg
     2641 aaccatgcgg cgaaattcga tcaaactcac gctcggaccg gcagacgaca gccgtggctc
     2701 ggtaagaacc ggagtaggat tggccaggac cacttttctg tccagggaac gcaccggtct
     2761 gatcgtggtt ggcatcatgg tcctgatctg gctctcgagc aacgacatca gtcaggatcc
     2821 gataccgatg cggtgggctc gaggcgaggc cggcggcatg acgctcgggc tcacaatctc
     2881 gccggacgga acgacaatcg caaccgtcga cagcgctggg cgagtggcga tttgggacaa
     2941 agaggacgac tggcggatca gccgatttct ggactatggc ggacttgctt ggagcgtggc
     3001 cttctcgccc gacggccgat tcctggcggt aggtggtggg gataccgcca tcgtcatgtt
     3061 cgacttgtct acaggggcaa cccctcgtgc gctcaacgcg ccggcaggtc agaccaaggc
     3121 tttggcgttc tcgcctgacg ggacgactct cgcggcgacg agcggacgga atggtgagat
     3181 cgtcctgtac gatctcggcg caggccgagt gcgcacgacc ctcttgggtg accttccagc
     3241 catgagtatc gcattctcgc ccgacggtcg gtctctcgcg tcaggtgcaa gagacaagaa
     3301 aaccgttaag ctctgggatc tcgaaaccgg ccgcagccgg atacttttca gggacgctag
     3361 tggcccgatc agctcggtcg ctttttcgtc tgacgggagc cttctggccg cggcttgttc
     3421 gatcgatcag ctcgtccggc tctgggacgt tgactcgggt ctattgtggc gtcagtttga
     3481 gggtcacaga ggcggcacca actcggtcaa tttctcaccc gatggagaga ctttgaccac
     3541 atcgggcagt gacgggttcg tcaagctttg gagagtcgcc actggcgagc agctcgccag
     3601 tctggatggt caatccttgt ggctgcccaa cctggcattc tctgccgatg gccaaacgct
     3661 ctttgcaacc ggcagcgaca acaatatccg g
//
LOCUS       DATAQL010000723         4577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_990765, whole genome shotgun sequence.
ACCESSION   DATAQL010000723 DATAQL010000000
VERSION     DATAQL010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990765"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            567..2777
                     /locus_tag="VHS97_12600"
     CDS             567..2777
                     /locus_tag="VHS97_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449089.1"
                     /translation="MRRQVRLKFAGLTMGLALLCAAIGAAPAPSSYLGVERTIESIRR
                     SWSSPGAPAQPNRPGWDALFDALLGDLKTYATAESDSERQQALDRINQISNSLGAVAW
                     TPAATLRGEIEQWMSPRLRVAWAKRRLSDTVAALPATNDPTIRANRARWLGFVQNDLG
                     NALRDYDSATTVSQRQAALHRLHESLGSLENGNRSRPWWPSWELQTAVNDLFNRPNLD
                     VAIDVNTVSPLFNANLVETGPVFRKGYWSQVTAGPKTGFGLLPSDDGIAFYNKQSLVS
                     VTPITDFQNQIAADDQGRRAAKLYQFSATTYDWSELTITTVLRSSGLTIIPSSTHNID
                     AAIGSAPTAGGGLGRGIAALIGLNQDAITGKVKEGAMPRFQQQIPVEAQEEALERIGR
                     ETAQRNADLRSKGLVGNNTVAVRDFLITQLSLRSRPDAVFVGGLFQFRDAPGQLGADA
                     QKPSSLAATYEPGGVSALVHLSSLLSSAAAGVYQRDEVKSVDNLMLSVADLPPGTAPR
                     DAVKLTKNVDFATYAKAVDEGRKPGSPRVTVLRITRPPSAPELTVDARGFLVALIHDL
                     QIEVPAPESEARGGVVGAAAKIYRIKIPHAEVALSYQVDSASDKATQLHAKVQDFSPG
                     TNAEVLAIADDEAKAVPLSRFSAAVVLGAIGGRLRTLPIDVRLDQLKLPGFSLRSISP
                     LDPSGWVRVGLLRNPDAPLQLTQTAPPAPAPPAPVSQPASSQPVSLVPTVAGQH"
     gene            complement(2800..3429)
                     /locus_tag="VHS97_12605"
     CDS             complement(2800..3429)
                     /locus_tag="VHS97_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3449090.1"
                     /translation="MTFADLRKRLGGIPLDRVWFNPAPGTATEKDVLDVEARENRLCE
                     LVDGTLVEKAMGFEEARVALRLGRLIDTFVDQNDLGVCVGADGMMRIAPDLVRIPDLS
                     FISWDRLPGRESPKAPIPDLAPDLAVEVLSEGNTRAEMARKVREYFEAGVRLVWLIDP
                     KKRTARVFSVPERSVLIRADQTLDGGEVLPGFVIPLSDLLDRGRRPRLR"
     gene            3621..>4577
                     /locus_tag="VHS97_12610"
     CDS             3621..>4577
                     /locus_tag="VHS97_12610"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3449091.1"
                     /translation="MPSIPTLIGNQAPPFDLPCTRFSDPGRSRATLEDYRERWLVLVF
                     YPRDFSLICPTELIGLSQRFDEFAEQNCDLLGISCDSVESHERWIATPLKRGGLGGLS
                     FPLASDLDGSAARAYGVYQEQDHVAVRGLFIIDHTGIVQYQVVHSLSVGRRSQEVLRV
                     LAALQSGGLCREDWMADSPTIDPFETIRPGRYFSHYSIEAEAGSGTFARVFRARDLQL
                     DRSVALKVFKPDCPVTPSAALAEARAAAALNHPNLCTIYAVDDSAGVPIISMEYIAGE
                     SLSKAVKPGRLGAEALLSISGQIASGMAAAHAEGIVHGDLKPE"
BASE COUNT          889 a         1451 c         1366 g          871 t
ORIGIN      
        1 acggacgatg agccggcgcc acaccccgac agaccggcgc tcgacccaac taccatcaga
       61 atgaatgaca ggcgaaacgg ccatgccgtc ggcgtcggtc gactctcacg cataaacgct
      121 atctcctagc tatatcacga acggcggatt cgaagatgat aaatcaatga ggtggcgaaa
      181 ctgtgcagca ggtccccgga ccgcgaacgg tgctcaaaaa tgccggcgcc ccgaccaatc
      241 ttgattgtaa catccaggcc ccctgcctgt cccaggcgat tacgaccggc ggcaacgcaa
      301 tcctctcctt cgatacaacc gttctgaccc tcgcaagagt gcagggagca aagaaatcct
      361 gtgtgttgtc cgactggcgc cgattctgca tgtgctaaag ttcgcctttt tcctggccga
      421 acaacgcgtg acggcggaaa cttaggcaga gtagccggtt cctgctaaaa tgctgcgggt
      481 attatcaacc agcgatcgtc atcgagacga tgcaagtagg aaccaggatt cgagaaactg
      541 caacggatta ccgacggaga cgacgcatgc gacggcaagt gaggctcaaa tttgctgggc
      601 tgacgatggg gttggcgcta ttgtgcgcgg caatcggtgc tgcgcccgcg ccgtcgagct
      661 atctgggggt cgaacggaca atcgagtcga ttcgacgatc ctggtcgagt ccgggtgcgc
      721 cggctcagcc caaccgaccg ggctgggacg cgcttttcga tgcgttgctt ggcgatttga
      781 agacttatgc cactgccgaa tccgacagcg aacgccagca ggcgctcgac cggatcaatc
      841 aaatctccaa tagcctgggg gcggtcgcct ggacgccggc ggcgaccctg cgtggcgaaa
      901 tagagcagtg gatgagtccg aggttgcgcg ttgcgtgggc caagcgccgg ctgagcgata
      961 cggtcgctgc cttgccggcg acgaatgatc ccaccatacg ggccaaccgg gcgcgctggt
     1021 taggcttcgt gcaaaacgac ctgggaaacg cgctgcgcga ttatgactcc gctaccaccg
     1081 tctcccagcg gcaggccgca ttgcatcgcc ttcacgaatc gctagggtcg ctcgaaaacg
     1141 gcaataggag tcgcccttgg tggccgtcgt gggaacttca aaccgcggtc aacgatctgt
     1201 tcaaccggcc taacctggat gtcgccatcg atgtgaatac cgtctcgccg ctcttcaatg
     1261 ccaacctcgt tgaaaccggt cccgttttcc ggaagggcta ctggtcgcag gtcactgcgg
     1321 gacccaagac cggctttgga ctcttgccca gcgacgacgg catcgcgttt tacaacaaac
     1381 aatctttggt gagcgtaacg ccgatcaccg actttcaaaa ccagatcgcc gcggatgacc
     1441 agggacgccg cgcagccaag ctctaccaat tcagcgcgac cacgtacgac tggtccgagc
     1501 tgacgatcac taccgttttg cgcagttcgg ggctcacgat tataccctcg tccactcata
     1561 acatcgacgc cgccatcggg tcggccccga ccgccggcgg cgggctggga cgcggcattg
     1621 ccgccctgat cggtctcaac caggacgcga tcaccgggaa ggtcaaggaa ggcgccatgc
     1681 ctcgttttca gcagcaaatt cccgtcgagg cccaggaaga ggcgctcgag cgcatcggcc
     1741 gcgagaccgc gcaacgcaat gccgacttgc gctcgaaggg cttggtcggc aataacaccg
     1801 ttgccgtccg cgatttctta attacccagc tctcactgcg gtcgcgtccc gacgctgtgt
     1861 tcgtgggagg cctgtttcaa tttcgcgacg caccggggca gcttggcgcc gacgcgcaaa
     1921 agccttccag cctggccgct acctatgaac cggggggcgt gtccgccctt gttcatctga
     1981 gctcgctgct ttccagcgct gcggcgggag tgtaccagcg cgatgaggtc aagtccgtcg
     2041 acaatctgat gctcagcgtc gccgatctac cgcccgggac cgccccgcgt gacgcagtca
     2101 agctgaccaa aaacgtcgat ttcgcgacgt acgccaaagc ggttgacgag gggcgcaagc
     2161 ctggatcccc cagggtgacg gtgctgcgaa tcacgcgccc accatcagcc cccgaactga
     2221 ccgtcgatgc caggggcttt ttggtcgcgc tcattcatga cctccaaatc gaagtccccg
     2281 cgcccgagag tgaagccagg gggggcgtgg tcggcgcggc cgccaagatc taccggatca
     2341 agattcctca cgccgaagtc gccctgtcat atcaagtgga ttcggcgagc gacaaagcca
     2401 cgcaactgca cgccaaggtc caggacttca gtcccggcac caacgcggag gtcctggcga
     2461 tcgccgatga cgaggccaag gccgtcccgc tgtcgcgctt ttctgcggct gtcgtcttgg
     2521 gtgcgatcgg cgggcgattg cgaactctgc cgatcgatgt cagactcgac caactcaagc
     2581 ttccaggctt ctcgctccgg tcgatctcgc cgctcgaccc tagtggttgg gtccgcgtgg
     2641 gtctcttgcg gaaccccgat gcaccgcttc agttgactca aacagcgccc ccggctcccg
     2701 ccccgccggc gcccgtcagt caaccggcgt cgtcccagcc agtgtccctt gttcccacag
     2761 tcgcgggaca gcactgaggg agaccgactt agccggcgat cagcgcagcc gcggtcgccg
     2821 gccacggtcg agcaggtcgc tgagtggaat tacgaagccc ggcaggacct cgccgccgtc
     2881 gagcgtttga tccgcgcgga tgaggactga cctttcggga acggaaaaga cgcgtgccgt
     2941 gcgtttcttg ggatcgatca accagacaag cctgacaccg gcctcgaagt attcccgcac
     3001 cttgcgggcc atctcagccc ttgtgttccc ctcgctgagc acttcgacag ccaggtcggg
     3061 cgccagatcc gggatcggcg ctttggggct ctcacggccc ggtagtcggt cccagctaat
     3121 aaacgacagg tcgggaatcc gtaccagatc cggtgcgatc cgcatcattc cgtcggcacc
     3181 gacgcagact ccgaggtcgt tttggtcgac aaaagtgtca atcaggcgtc ccaggcgcag
     3241 ggccactcgg gcttcctcga atcccattgc cttctccacc agggtaccgt cgaccagctc
     3301 gcagagccgg ttttcacgag cctcgacatc gaggacatct ttttcggttg cggtccccgg
     3361 cgcagggtta aaccagaccc gatcgagcgg aatgcccccc agccgctttc gcaagtcggc
     3421 aaatgtcatg atggtctcct cgcggtccgc cggcgaattc aaagtgaatt ttgacaccgc
     3481 gcggtttttc ggggaaggat cttttgttca ttagccgccc aggccttccg caagccgcgc
     3541 actcccaggc gcagagataa ttccttagaa tgtagaattc acgagtcgac aacagccccg
     3601 aggagcgagc gcgtccaccc atgccgagca tcccgacact gatcggtaac caagctcccc
     3661 ccttcgattt gccgtgcact cgcttttccg acccaggcag aagccgggcg accctcgagg
     3721 actatcgaga gcggtggctg gtgcttgtct tttatccacg cgatttttcc ttgatatgtc
     3781 cgacggagtt gattgggctc tcccagcgat tcgatgaatt cgcggaacag aattgcgacc
     3841 tgctggggat cagttgcgac tcggtggaat cgcacgagcg atggatcgcg actcccttga
     3901 agcgcggcgg cctgggaggt ctgagttttc cacttgcgag cgatctggat ggatccgccg
     3961 cgcgggctta cggcgtgtat caggagcaag atcacgtggc ggtgagaggg cttttcatca
     4021 tcgaccacac tggtatcgtg cagtatcagg ttgtccacag cctgagcgtc ggccggcgca
     4081 gccaggaggt cttgcgcgtt ctggccgccc tgcaatcggg cggattgtgc cgcgaggatt
     4141 ggatggcgga tagcccaacg atcgacccat tcgagacgat ccggccggga cgatactttt
     4201 cccattattc gatcgaggcc gaagcgggct caggaacttt cgcgcgggta ttccgtgcgc
     4261 gcgacctgca gcttgaccgc tcggtcgcgc tcaaggtctt caagcctgac tgtccggtaa
     4321 ccccgagtgc cgcccttgcc gaggcacgcg cggccgccgc gctcaatcat cccaatctgt
     4381 gcacgatcta cgccgtcgac gattctgcgg gtgtccctat catcagcatg gaatatatcg
     4441 ccggcgaatc attgtcaaag gcagtcaagc cgggccgcct gggggcggaa gcgctcttat
     4501 cgatttccgg ccagatcgcc tctgggatgg ccgccgcgca cgccgagggg atcgtgcatg
     4561 gcgacttgaa gcccgag
//
LOCUS       DATAQL010000724         6318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2975412, whole genome shotgun sequence.
ACCESSION   DATAQL010000724 DATAQL010000000
VERSION     DATAQL010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975412"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VHS97_12615"
     CDS             <1..1166
                     /locus_tag="VHS97_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX3449092.1"
                     /translation="DVIAREILNHGVTHLQLIPSLARMLATDPRSLAALGSLKKLLLG
                     GEVLPASLVHRLRGSTTADIYNMYGPTETTIWSTAYLIPESADFANVVPIGRPLGNTR
                     AYILDSQFQLIAEGEPGELFLGGLGVARGYLHRPDLTAERFVPDHFAWEGFMYRTGDV
                     ARCRPDGNLEFLGRTDFQVKLRGHRIELGEIERALEQLGSVSQAVVVAREDRPGDKRL
                     VAYIIARHGQSVTQATCRSALESKLPGYMVPSHFVFLDRLPLTPSGKIDRNSLPVVRD
                     LGAATELAPSSGGGPFDELQRIIVNAWREALGMAEIGLDENWFDLGATSLMMPQVQMD
                     LQRELGREIPLVDLFEFHTVNALAAHLGGESTTQRKSDRALRRLAALRQKGPA"
     gene            1163..>6318
                     /locus_tag="VHS97_12620"
     CDS             1163..>6318
                     /locus_tag="VHS97_12620"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX3449093.1"
                     /translation="MRNSAIAIVGMAGRFPGARNIDEFWRNLRDGAESVRQLSSAELL
                     AAGVSPDLVARPDYVKAAAVLEGVDMFDASFFGLSPRDAAIMDPQHRHFLECAWEAIE
                     NAGHPPDAFAGSIGVFAGCGTPSYLIHNLIHNRHLMDSAGLFLIRQTGNDKDVLATRV
                     SYQLDLRGPSLTVQTACSTSLVAVHQACQSLLNCECDMALAGGVTIEIPHGHGYLYRE
                     GEILSHDGHCRAFDAASSGTVFSSGVGIVVLRRLDDAIADLDTIYAVILGSAVNNDGQ
                     RKVGYLAPSVAGQAEAVAEALDVAGVNAETITYVETHGTGTAVGDPLEIKGLTQAFRG
                     DTARTGYCAIGSLKTNVGHLDTAAGVAGLIKTALALRHQQIPASLNFQNANPQIDFGS
                     TPFYVNTSLIDWKSDGTPRRAGVTALGIGGTNAHVILEEAAELKRTGVSHSHQIIPLS
                     ARTETALEAAGAQLTLYLRDNPEVRLTDVAFTCQVGRKAFRHRRVVVASSSTEAASLL
                     ADPESPRQFTGVAVERAPWSVVFLFSGQGSQYPNMGADLYNHVPLFRNCLDRCAEELR
                     PHLGLDLRGMLFPNAADTASAAQQLNRTAFTQPALFSLEYSLAQWWMALGVRPDAMLG
                     HSIGEYVAACLADVMSLEDALAITSIRGRLMEECPAGSMLAVSLSREELDLPPDLALA
                     ASNAPEQCVVSGPLDALTDFEKTLVQNNVSCRRLQTSHAFHSAMMDSILESFVERMSQ
                     IALRPPRIPYLSSLTGRWITAIEATDPNYWASQLRNTVQFADGVSELLRQPGRVMIEI
                     GPGQTLASLVRLQTGNSSAGDTAKVFSSLPRREQAVGDTAFFLQTLGQLWIGGQSVDW
                     HALHPHEALSRIPLPTYPFERQRYWIDPDKPAPPETAEASVGSSALAPLSGVETPETA
                     GRERRHRIGKWFYRRAWQRTLRPPAEAPPPACWVVFLEPNDIGKQICARLQDVAHRLV
                     MVTPGRRFKRSRHGQYTIRPGMRDDYDALLAELARRQISPARIVHLWSTLKVTQKLSL
                     DEKLDRCFYSLLFLAQALGAQDMTGIEIGIVSDCLHCIHGEPVADPVCATLLGPTRVI
                     PKELPGLTCRGIDIDLSSQGPARAADQIIAELCTPFRESVVAIRRGERWIESLEPAEL
                     RSSMDFGRLRKNGVYMITGGLGDLGLVIAEELAREFEARLVLLGRTPFVPAHEWRHAL
                     KSSDTPDRLKSRIAKLVELESLGAEILYIPCDVGRRDEMERAIDTARARFGAIHGVIH
                     AAGVIDDGPLLIKSRASAASVLDPKVKGTLVLIDVLTAAMADTKHNEPLDFIALFSSV
                     SSLSAPAGQVDYIAANAFLNAFAASRRDVRVVAINWDAWRDVGMAARASTAHPLLGRR
                     LVDTGGEILYKATLCRERHWMLAEHCLKTGQAVLPGTGYLEMASAALTNGSFDQGVAF
                     EDVFFQSPFLADPAQSREALVDLNRTHNGTFQFSVRTGDHGSIEHASGRIERCHKPPP
                     GRVALEAIRARCQSRYVTFDESHRTHQEQYFDFGPRWRCLSAIHIGTNEALAVLALPA
                     AFAGDTAEYHLHPALLDLATGSALYLIADYGPSSPVYFPIYYKSAVVFRTIPARFFSH
                     IRSRQRNEADRDVATFDLTLVDLEGMVLAQIDGFSMRQMRDPREGLGIVGAHVSAAAD
                     RNGFVDDVLERSIVPDEGAKAFTNIISSQTSHELFVLPDGPGGVIRQPEYV"
BASE COUNT         1177 a         1961 c         1877 g         1303 t
ORIGIN      
        1 cagacgttat cgcgagggag atcttgaacc atggtgtcac acaccttcag ctcatccctt
       61 cgctcgcccg aatgctagcg accgaccctc gttctctcgc cgctttgggc tctctcaaga
      121 agctgttgct ggggggtgaa gtgctgccgg cgtcgctcgt gcacaggctg cgcggctcga
      181 caacggctga catctacaat atgtatggtc cgaccgaaac gaccatctgg tcaaccgcat
      241 acctcattcc ggaatccgcc gatttcgcca atgtcgttcc catcggccgg ccacttggca
      301 atacgcgtgc ttatatcctc gattcccaat ttcagctcat tgccgaggga gagcccggcg
      361 agttattcct gggcggcctt ggggtggcgc ggggttatct gcatcggccc gaccttactg
      421 ccgagcgctt tgtccccgat cactttgcgt gggaaggctt catgtaccgc accggtgacg
      481 tcgcgcgctg tcgaccggac ggtaatctcg agttcctggg gcggaccgac tttcaggtca
      541 agctaagagg gcaccgaatc gagctgggcg agatcgaaag agcgctcgaa cagcttgggt
      601 cagtcagcca ggccgtcgtc gtggcgcggg aagatcgacc gggagacaag cggcttgtcg
      661 cctacattat cgcccgtcac ggccaatccg tgacgcaggc aacttgccgg tccgcgctcg
      721 agtccaagct gcctggatac atggtgccct cacactttgt ctttctggat cggcttccac
      781 ttactcccag tggcaagatt gatcgcaact cgctgccggt agttcgcgat cttggagccg
      841 ccacagagct cgcaccgtcg tcaggcggag gcccatttga cgagcttcag cgcatcatcg
      901 tcaacgcatg gagggaagct ctgggaatgg cggagatcgg cctggatgag aactggttcg
      961 atctgggggc gacgtcactc atgatgccgc aggtgcagat ggatctgcag cgtgaactgg
     1021 ggcgggagat accgctcgtc gatttgtttg aatttcatac tgtgaacgct ctcgccgccc
     1081 atctgggggg agagtcgacc acgcaacgca aatccgatcg cgcactgcgc cgcctggccg
     1141 cgctgaggca gaaagggcct gcatgagaaa ctcggcgatt gccatcgtgg gaatggccgg
     1201 gcgatttccg ggcgctcgca atatcgacga gttttggcgg aacctgcgcg acggcgccga
     1261 atcggttcgt caactgagct cggccgagct tcttgccgcc ggtgttagtc ccgatcttgt
     1321 ggcccggccc gactatgtaa aagccgccgc ggtactcgaa ggtgtcgaca tgttcgacgc
     1381 gtcgttcttc gggctgagcc ccagggacgc ggcgatcatg gaccctcagc accgtcattt
     1441 tctggaatgc gcctgggaag ccatcgaaaa tgccggacac ccacccgatg ccttcgccgg
     1501 gtcgatcggc gtattcgcgg gctgtggaac gccctcgtac ttaatccaca acctgatcca
     1561 caaccgccac ttgatggaca gcgccgggct tttcctgatc cgtcagactg gaaatgacaa
     1621 agacgtcctc gccacgcgcg tgtcgtatca actggatctg cgcggaccaa gcttgaccgt
     1681 gcagacggcc tgctcaacct ctctcgtcgc ggtccatcag gcctgccaga gcttgctcaa
     1741 ctgcgagtgc gacatggcgc tggccggtgg cgtcacgatc gagatccccc acggccacgg
     1801 ctatctctat cgcgaggggg agatcttatc tcacgatggc cattgtcgcg ctttcgatgc
     1861 ggcgtcgagc gggaccgtct tttccagcgg cgtcggcatt gtcgtcctgc gccggctcga
     1921 cgacgccatc gccgatctcg acacgatcta cgcggtcatt cttggttcag ccgtcaacaa
     1981 cgatggccag cgcaaggtgg gatacctggc gccgagcgtc gccggtcagg ccgaagccgt
     2041 tgccgaggcg ctggatgtcg ccggcgtcaa tgcggaaacg atcacatacg tcgagaccca
     2101 cggcaccggc accgccgtgg gcgatccact tgagatcaag ggactcactc aggcatttcg
     2161 tggtgacacc gcacgcacgg gctattgcgc gatcggttcg ctcaaaacca acgtggggca
     2221 cctcgacacg gcggccggtg tcgcgggcct catcaagacg gcattggccc ttcggcatca
     2281 acaaattccc gcaagtctca actttcagaa cgctaatccc cagatcgatt ttggatcaac
     2341 cccgttttac gtcaacacgt cactcatcga ttggaagtca gacggcacgc cgcgtcgcgc
     2401 gggcgttacc gcgctcggga tcggcggtac caacgcacat gtcattctcg aagaggcggc
     2461 cgagctgaaa cgaacgggag tctcgcattc gcaccaaatc atccccctgt cggcccggac
     2521 cgaaaccgca cttgaagcgg ccggagcaca gctcacgctg tatctgcgcg acaaccctga
     2581 agtacggctc accgatgttg ccttcacgtg tcaggtgggt agaaaggcgt ttcgccatcg
     2641 gcgcgtcgtg gttgcgagca gctcgactga agccgcctct ctgctggcgg atcccgagtc
     2701 cccgcgacaa ttcacgggcg tcgccgttga acgcgctcct tggtctgtgg tgtttctgtt
     2761 ttccggtcag ggttcccagt atcccaacat gggggccgac ctttacaacc atgtgccttt
     2821 attccgcaat tgtctcgatc gatgcgcgga agagcttcgg ccgcatctgg gtctggattt
     2881 gcgcggcatg ctatttccga acgccgccga tacggcctcg gccgcgcaac agttgaaccg
     2941 aaccgccttc acacaacccg ctctcttttc cctggagtat tcgctggcgc aatggtggat
     3001 ggccctcggt gtcaggccag atgcaatgct tggtcatagc attggggagt atgtcgcggc
     3061 gtgccttgcg gacgtgatgt cgctcgaaga cgcgctggcc atcacctcga ttcgtgggcg
     3121 attgatggaa gaatgtccgg ccgggtcgat gctggccgtg tcgttgtcac gggaagaact
     3181 cgatctcccg cccgacctgg cacttgccgc ctcgaacgca cccgagcagt gcgtggtgtc
     3241 cggtcctcta gacgcgttga cggatttcga gaagacctta gtccaaaaca atgtatcgtg
     3301 ccgccggctc cagacctcac atgcctttca ctccgcaatg atggactcga ttctcgagtc
     3361 gttcgtcgag cgcatgagcc agatcgcgct gcgaccaccg cgaatcccct acctctcgag
     3421 cctgaccggt aggtggatca ccgcgataga agcgactgat ccgaattatt gggcaagcca
     3481 gctgcgcaat accgtgcaat tcgctgatgg cgtcagcgag ctgctgcgcc agcctggtcg
     3541 cgtcatgatc gagattggtc caggccagac tctcgcctca ctggtacgac tgcagaccgg
     3601 caacagttcc gcaggcgata cggccaaggt tttttccagt ttgccgcgcc gcgagcaagc
     3661 ggttggggat actgcgtttt tcctacagac attaggccaa ctctggatcg gcggccagtc
     3721 ggtcgactgg catgccctgc atcctcacga agcgctatca cgtattccct taccaacgta
     3781 tccgttcgag cggcaacgct actggatcga tcccgacaaa ccggcgccgc cggagactgc
     3841 ggaggcgagt gttggatcgt cggcgcttgc gcccctctct ggtgtcgaaa cgccggagac
     3901 ggcaggtcgt gagcgccggc atcgcatcgg aaaatggttt tatcgccgcg cctggcagcg
     3961 cactcttcga cccccggcgg aagcaccgcc gccggcgtgc tgggtcgtct ttctcgaacc
     4021 aaacgatata ggcaaacaga tctgcgctcg attgcaagac gtcgctcatc ggcttgtcat
     4081 ggtaaccccc ggccgccgat tcaagcgcag ccggcacgga cagtatacga tccgccctgg
     4141 catgcgcgac gattacgacg ccttgctcgc cgaactggcc cgacgacaga tttcgcccgc
     4201 aagaatcgtg catctctggt ctacgctaaa agtcactcaa aagctctcgc tggatgaaaa
     4261 gctcgaccgc tgtttctata gccttctctt tttggcgcag gcgctcggcg cccaggatat
     4321 gacgggtatc gaaatcggca ttgtctcgga ctgtctccat tgcatccatg gtgagccggt
     4381 cgccgatccc gtctgcgcga ccctgctggg ccccacgcgg gtgattccca aagaactccc
     4441 gggactgacc tgtcgcggta tcgatatcga cctgagcagc cagggtccgg ctcgggccgc
     4501 cgatcaaatc attgccgaac tgtgtacacc cttccgcgaa tcagtggtcg cgatccgccg
     4561 cggcgaacgg tggattgaaa gcctcgagcc cgcagaactt cgctcgagca tggacttcgg
     4621 gcgcctgcga aagaacgggg tgtacatgat cacgggtggg ctgggcgatc tcggtttggt
     4681 gatcgccgaa gagctcgccc gagagttcga agcccggctt gtgctgctgg gacgcacgcc
     4741 gttcgttcct gcccacgagt ggaggcacgc gctcaaatcc agcgacacgc cggatcgcct
     4801 caagtcgcgg atcgccaagc tcgtcgagct cgagtcattg ggggccgaaa tcctctacat
     4861 tccctgcgat gtcgggcgcc gcgacgagat ggagcgcgcc atcgacacgg cacgggcgag
     4921 attcggcgcg atccacggag tgattcacgc ggcgggcgtg attgatgacg gtcctctctt
     4981 gatcaagtcg cgcgcgagcg cggcgagcgt actcgatccc aaggtcaagg gaactctcgt
     5041 actaatcgat gttctcacgg ccgccatggc tgacacaaag cataacgagc cgctcgattt
     5101 catcgcgtta ttctcttccg tcagctcact gtcggcgccc gccgggcagg tggattacat
     5161 tgcggcaaac gccttcctga atgccttcgc cgccagccgc cgcgatgtgc gtgtcgtcgc
     5221 aataaactgg gatgcgtggc gcgatgtggg gatggcggcg cgcgcgtcga ctgctcatcc
     5281 gctcctcggt cgccgactgg tcgatacagg cggcgaaatc ctttacaaag cgacgctctg
     5341 tcgcgagcgt cactggatgc ttgccgagca ctgcttgaag acgggccagg cggtacttcc
     5401 gggcactggc tacctcgaga tggcgtccgc ggctctgacg aatggttcgt tcgatcaggg
     5461 cgtggcgttt gaagatgtgt ttttccaatc gcctttcctt gctgatcctg cccaatcaag
     5521 agaagcgctg gtcgatctga accggacaca caacggaact tttcagttct cggtccgaac
     5581 aggcgatcat ggatcgatcg agcacgcgtc ggggcgaatc gagcggtgcc acaagcctcc
     5641 tcccgggcgc gtcgcacttg aggcgatcag ggcacgatgc caatcgcgct atgtcacgtt
     5701 tgatgagtcc caccgcacgc atcaagaaca gtattttgat tttggtcctc ggtggcggtg
     5761 cttgagtgcg atccacatcg gaaccaacga agcgctcgcg gtgctcgcgc taccggccgc
     5821 gtttgccggc gacacagcgg agtatcacct ccatccggct ctcctcgatc tggcgacggg
     5881 ctcggccttg taccttatcg cggattacgg gccgtcgagc ccggtctact ttcccatcta
     5941 ctacaagagc gccgtcgtct ttcgcactat tcctgcccga ttcttcagcc acatccgttc
     6001 gcgtcagcga aacgaagctg atcgggacgt ggcaaccttc gatttgactc tcgtcgatct
     6061 tgaaggaatg gtgcttgccc agatcgacgg attctcgatg cggcagatgc gcgacccgcg
     6121 tgaaggcctt ggcatcgtgg gagcgcacgt atccgctgcc gccgatcgga atggattcgt
     6181 cgacgacgtc ctagagcgct ctatcgttcc tgacgaggga gcgaaggcat tcacgaacat
     6241 catctcgtcg caaacgtcgc acgagttgtt tgtcctgccc gatggacctg gcggagtcat
     6301 tcgtcaaccg gaatacgt
//
LOCUS       DATAQL010000725         3616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1295767, whole genome shotgun sequence.
ACCESSION   DATAQL010000725 DATAQL010000000
VERSION     DATAQL010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295767"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2152
                     /locus_tag="VHS97_12625"
     CDS             <1..2152
                     /locus_tag="VHS97_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3449094.1"
                     /translation="DEDFVSSPDLDFHPTDVIEDADGSLVIVDTGGWYKLCCPTSQLQ
                     KPDVLGAIYRVKRRGAPEVADLWGKNLWARFVDPLRAMPSEAIVKLLDDPRPAVQRHM
                     IDFCSSPGRKDAILAMLREVVPKHASSRLRCNTVWALARIDDPGARALVRAFLNDSDE
                     TVRQVALHVTSLGRDRDAVPRLVELLQSSSLPNRHAAVEALGRIGDPSAVPALLKAAG
                     EVDDRILEHSCTYALIEIGDPKTTVAGLASKNDRTKKAALVALDQMDGGGLDPKFVAA
                     LLASPEPDMKETASWIIGRHVDWAVELAGVLGERLWRIDLPPAERTDLERQLGRFAQA
                     APIQRLLAVRLRDAFAGSAARRSSLQAMAWSNLKAETVPHEWVDAVAGVLEGDPATPE
                     LVAPAVATLRALPLTKKKAGDLPDRLLRIAGDTKNGVDLRLQALAAVPGGVVNPDQTL
                     VDFLVKELDRDRPVAARTTAADVLARAKLTAVQLVRLADLLPTAGPLEVDRLLTAFEQ
                     STDEALGLKLVENLSKASALSSLRIDSVKASLAKYGPSVRKEAQALYTRLNVDLAKQK
                     EKLEQLMTTLSAGDVRRGQLVFHSEKAACYSCHAIGYRGGNVGPDLTKIGSVRADRDL
                     LEAIVFPSASFVRSFEPIAVATNDGKVFNGLLRGETADELILATGVNQETRVTRRDIE
                     ELRPGTLSVMPAGLEQQLTPQELADLVAFLRACK"
     gene            2572..3525
                     /locus_tag="VHS97_12630"
     CDS             2572..3525
                     /locus_tag="VHS97_12630"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449095.1"
                     /translation="MRLQTWMYGIVVAGVATLATAASYADDKKADVLPGPIDSLSDLQ
                     DTAKLVFKLADTNNDQQISQKEAIDAGNLLVGGFFFRADVNGDGVLTAQEAQQARETL
                     YMQQPLLRFVVERAKPTNAAPGNQPQNLQPIVGGTTNDPARVVQSFTADPMGTIGNLL
                     DTNRDQKIEASELRQAVQTGVQTLFLVADTNQDGQLSPYELNAAVTEVAKSTVQTAFQ
                     VADGDRNGMLSMDEYDKALTEPVHAVFRVLDANGDNQLTLAELERAQQIIADQIRRLQ
                     VPEPSNSVSNQVQRGRNRAIMNQPVQAAPSPSPSSTPVAPR"
BASE COUNT          730 a         1137 c         1081 g          668 t
ORIGIN      
        1 agacgaagat ttcgtctcca gtcccgatct cgacttccac cctaccgacg tgatcgagga
       61 cgccgatggc agcctcgtga tcgtcgatac cggcggctgg tacaagctct gctgcccgac
      121 gtcacagctt caaaagcctg acgtgttggg ggcgatttat cgcgtgaagc gccggggcgc
      181 gcccgaggtt gctgatctgt gggggaagaa cctctgggcg cggttcgtgg atcctcttcg
      241 tgcgatgccg tctgaggcca tcgtcaagtt gctcgacgac ccacgtccgg ccgtgcagcg
      301 gcacatgatc gacttctgtt cctcccccgg gagaaaagat gccatcctgg ctatgttgcg
      361 cgaggtggtg cccaagcacg cctcatctcg cttgcgctgc aacaccgtct gggcgctcgc
      421 ccggatcgac gatcccggcg cccgagcctt ggtccgcgcc ttcttgaacg attcggacga
      481 aaccgtacgc caggtcgccc tccacgttac gagcctcggg cgcgaccgcg atgcagtgcc
      541 ccgcctggtc gaacttctcc aatcttcgtc tttgcccaac cgccacgcag ccgtcgaggc
      601 gctgggtcga atcggcgacc cgtcggccgt gcccgcgctc ttaaaggctg cgggtgaggt
      661 agacgatcgc attctcgaac attcgtgcac ttacgcattg atcgaaatcg gcgaccccaa
      721 aaccacagtg gccggcctcg cgagcaagaa cgaccgaacg aagaaggccg cgttggtcgc
      781 gctcgatcag atggatggcg gcggactcga ccccaagttt gtcgcagcgc tattggcatc
      841 gccagagccg gacatgaagg agacggcatc gtggataatc ggccgccatg ttgactgggc
      901 ggttgagctg gccggtgttt tgggtgaacg gctctggcgc atcgacctcc cgcccgccga
      961 gcgcactgac ctcgaacgcc agttgggccg gttcgcacag gcggctccga tccagcggct
     1021 tttggccgtc cgcctgcgcg atgcgtttgc tggatcggcc gcgcggcgga gcagcttgca
     1081 ggccatggcc tggtcaaact tgaaggccga gactgtgccg catgagtggg tcgatgcggt
     1141 ggcaggcgtg ctggaaggag accccgccac gcctgagctg gtcgcgccgg ccgtcgcaac
     1201 cctgcgtgcc ctgccactga ccaagaaaaa ggccggcgat ttgcccgatc gcctgctacg
     1261 gatcgctggt gacacaaaga acggggtcga cctgaggctc caggcccttg ccgccgttcc
     1321 gggaggcgtg gtcaatccgg atcaaacact cgtcgacttc ctggtcaaag agctcgaccg
     1381 cgatcggccg gtcgcggcca gaacgaccgc ggctgacgtg ctggcgcggg ccaagctgac
     1441 cgcggtgcaa ctcgttcgcc ttgctgatct gctgcccacc gccggaccgc tcgaagttga
     1501 ccggctgctg accgctttcg agcaatcgac cgacgaagcg ctcgggctga agctggttga
     1561 aaatctttca aaggcgtccg ccctttccag cctgagaatc gactcggtca aggccagcct
     1621 ggcaaagtac ggtccctcgg tgcggaaaga agcccaggcg ctctacaccc ggctcaacgt
     1681 cgatctggcc aagcagaaag agaaactcga gcagctcatg accacgctct cagcgggcga
     1741 cgtccgccgc ggccagctgg ttttccacag tgaaaaggca gcctgctact cctgccatgc
     1801 catcggctac cgcgggggca acgtcggacc agacctcaca aagatcggct cggtccgtgc
     1861 ggatcgcgac ctgctggaag ccatcgtttt tccgagcgcc agctttgtgc gcagcttcga
     1921 gccgatcgcc gtcgccacga acgacggtaa agtattcaac ggcctgctcc gcggcgaaac
     1981 cgccgatgag ctgatcctgg ctaccggcgt aaaccaggaa acgcgagtca cacgccgcga
     2041 tatcgaggaa ttgcgccccg gtaccctctc ggtgatgccg gccgggcttg aacagcaact
     2101 tacacctcag gaactggcgg acttggtcgc attcctgagg gcgtgtaaat gatctgcgca
     2161 ccgatcgtta agcgcactct cgatcgcgcc ctgaccagtg cgcgttgcgc aactcattgc
     2221 acgagaaccg gaaggacgac gcgcgtgagc cgcctcggac agtcatccat cgcgacacct
     2281 gtccactttg cttggttcca gccggcaaac ctcttgtcga gctaatggtt gcgccaatcc
     2341 gccgcgtcaa cgatcccggt gcactcgagc aaattcgggt gtaatcgctg attttccgac
     2401 ggttctgagc tttaaaagcg tcgtgcggca tctcggttgc agtacgagtt tctgccgaag
     2461 gcggccaggg atgaccaaga ctggatcgtg ttggtttagt ctggagacgc ctgcgctccc
     2521 accagggatg gtcgagcaga aatgattgca acagcgaagg agtaacgttt catgcgacta
     2581 caaacgtgga tgtacgggat cgtcgtggcg ggagtggcca cactggcaac ggccgctagc
     2641 tatgctgacg acaaaaaagc agacgtttta cccggtccga tcgatagcct ttcggacttg
     2701 caggacacgg ccaagttggt gttcaagctt gcggatacca acaacgacca gcagatttcc
     2761 cagaaagaag cgattgacgc tggaaatctg ctggtcggcg gatttttctt ccgggcagat
     2821 gtcaacggcg atggcgtttt gacagcccag gaggcccagc aggctagaga aaccctctat
     2881 atgcagcaac ccttgctcag gttcgtcgtc gagagagcca agccgaccaa tgccgcgccg
     2941 gggaaccagc cccagaatct ccagccgatc gtaggcggta caaccaacga tcccgctcgc
     3001 gtcgtgcaga gcttcacggc cgaccccatg gggaccatcg gcaacctgct tgatacgaac
     3061 cgcgatcaga agatcgaggc cagcgagctg cggcaggcgg tccaaactgg ggtgcagact
     3121 ctcttcctgg ttgccgacac caatcaggac ggacagctaa gtccctacga gctcaatgca
     3181 gccgtcaccg aggttgccaa gtcgaccgtt caaacggcat tccaggtggc ggacggcgac
     3241 cgaaacggca tgctgagcat ggacgaatac gacaaagccc tgaccgagcc cgtccatgct
     3301 gtctttcgcg tcctcgatgc gaatggcgac aatcagctca cactggccga gctggaacga
     3361 gcacagcaga tcatcgccga ccagattcgg cggcttcaag ttcccgagcc ctctaactcg
     3421 gtgtccaatc aagtccagcg ggggcggaac cgagccatta tgaaccagcc tgttcaagcg
     3481 gccccaagtc cttcgccgtc tagcacgccg gttgcgcccc gttaataact gtgacaaaaa
     3541 aagatcccgg gctgccagcc cgggatcttt tgtaaatact gccgacgagt cgactttacc
     3601 ctttcctaat cgttca
//
LOCUS       DATAQL010000726         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1448571, whole genome shotgun sequence.
ACCESSION   DATAQL010000726 DATAQL010000000
VERSION     DATAQL010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448571"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VHS97_12635"
     CDS             complement(<1..1104)
                     /locus_tag="VHS97_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3449096.1"
                     /translation="MTRTRTGLVPAALTLGVLPPPSSVRDLVSAKWDAIIVGAGHNGL
                     TCAAYLAKSGKRVLVLEARHRVGGACTLDQVWPGYRVSPCAYLVGLLHPLVIDELEMA
                     SYGFQWLPARAGMFVPFDDGTSVQLWDDDDLCEQEIRQFAPADFKGWQAFCGVKNRLR
                     DALRPPGDGDLWIGKAPSRDELERRLAGDHEARKMLFEWSMVECLEHYMSDERLQSAY
                     LGQGVIGTFASPHDPGTASIHFHHQSGRLGGTPGMWGYVIGGMGMVSFILCDIARDLG
                     AVVATGIPVARIIPGVGVELEGGERIEARCVVSNADPRATLRLLGDAADPVWQSRVEA
                     VPQVGCTVKLNVALNELPSFTARPGTNMPHHLGQ"
     gene            1223..2275
                     /locus_tag="VHS97_12640"
     CDS             1223..2275
                     /locus_tag="VHS97_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3449097.1"
                     /translation="MAADSAVVIKLSDLVDGQEAVCFAALVRKTRGTTKANQPFIKCL
                     FRDKRVTVEAPLWQDHRFFQDAESWTEGESYRLQVRGQFHVRYGMQIEILGARRAIDD
                     DAEDGYDFFDLVPNSVFPADELKKKLHVLIDRYIEDPRLKSLVETILAENDSLFSRMP
                     AAQNFHHSYTSGLLEHVWSMTRIAGFLADHYAKYYDQLDPPFNRGLIVAATILHDIGK
                     LRELEYHPVEAKYTIQGCLIGHVLMGRDMVREAARQIEGFSEELLLNLEHAILAHHGK
                     REFGSPVVPQTIEALLVSYIDDLDAKMNIMVRERMVSRTDDSFTERVFALDNRRIYKG
                     IPAAERDLLESDREPV"
BASE COUNT          507 a          749 c          788 g          507 t
ORIGIN      
        1 ctggcccaag tgatggggca tgttggttcc gggtcgggcc gtgaagctcg gtagctcgtt
       61 gagagccaca ttgagcttta cggtacatcc aacctgaggg acggcttcga cacgtgattg
      121 ccagaccggg tcggcggcat cgcccagcag ccgaagtgtc gcgcgcgggt ctgcgttcga
      181 gacaacgcaa cgagcttcga tccgctctcc tccttcgagc tcgacgccca cgccagggat
      241 gatccgggcc actggaatgc cggtcgcgac cacggcaccc aggtcgcgcg cgatgtcgca
      301 gagaatgaac gagaccatac ccattccgcc aatcacatag ccccacatgc cgggtgttcc
      361 tcccagccgg cccgattgat ggtggaagtg aatggaggct gtgccggggt cgtgcgggct
      421 ggcgaaagtg ccgatgacac cctgaccgag ataagccgat tgaaggcgct catcgctcat
      481 gtagtgttcg aggcattcga ccatcgacca ctcgaagagc atcttgcgag cttcgtgatc
      541 gccggcgaga cgtcgttcga gttcatcgcg ggatggggct ttgccgatcc acagatcgcc
      601 gtcacccggc ggtcgcaggg cgtctcgcaa ccggttcttg acgccgcaga acgcctgcca
      661 gcctttgaaa tccgctggtg cgaactggcg gatttcctgc tcgcagaggt catcgtcgtc
      721 ccagagctgg acgctggtgc catcgtcgaa cgggacgaac atgccggctc gggccggtag
      781 ccattgaaag ccgtagctgg ccatctcgag ttcatcgatg accaggggat ggagcagccc
      841 gaccaggtag gcacaggggg acactcgata gcccggccag acttggtcga gcgtgcaagc
      901 gcctccgacg cgatgacgag cttcgagcac cagcacccgc ttgcccgatt tcgccaggta
      961 agccgcacag gttaagccgt tgtggccggc gccgacgatg atcgcgtccc atttcgccga
     1021 aaccaggtcc cggaccgaag acggcggcgg cagaactccc agagttaggg cagcgggtac
     1081 caggcccgtt cgtgtcctcg tcatctaagc ccccgaggtc gcggtgcgtt gtttgaaccg
     1141 gctctggatg ttgtaaactg aggttggtga atcatgcaac ggtttcggcc ggcaatgccg
     1201 gcttgggaaa gggtggcaag atatggcggc agatagtgcg gtggtaatca agctatctga
     1261 cctggtcgac ggccaggaag cggtctgctt cgcggctctt gtcagaaaaa cacggggaac
     1321 aaccaaggca aatcagccct ttatcaagtg tctgttccga gacaagcgcg tgacggtcga
     1381 agcgcccctg tggcaagacc atcgcttctt ccaggatgcc gaatcgtgga cggagggcga
     1441 gtcctaccgc ttgcaggtgc gcggccagtt tcatgtgcgc tacggcatgc agatcgagat
     1501 cctgggcgct cgccgcgcca tcgatgacga cgccgaggat ggatacgact ttttcgacct
     1561 ggttcccaac agcgtctttc cggccgacga gctgaagaag aagctgcacg tcctgatcga
     1621 tcgttacatc gaggatccgc ggctcaagtc actggtcgaa acaatcctcg cagagaatga
     1681 ttcccttttt tcgcggatgc ccgccgctca gaatttccac cattcctaca cgtccgggct
     1741 tttggagcat gtttggagca tgacccggat cgccggcttc ttggccgatc actatgccaa
     1801 gtactatgac cagctcgatc cgcctttcaa ccggggtttg atcgtcgccg cgaccatctt
     1861 gcacgacatc ggtaaattgc gcgagctcga gtatcacccg gtcgaggcaa aatacacgat
     1921 acaaggctgc ttgatcgggc atgtattgat gggcagggat atggtccgcg aggcagcgcg
     1981 tcagatcgaa ggattttccg aagagctgct cttgaacctc gaacacgcga ttctggcaca
     2041 tcacggcaag cgtgagtttg gctcaccggt ggttccccag acgatcgagg ctctcctcgt
     2101 ttcctatatc gacgacctgg atgccaagat gaatatcatg gttcgcgagc gaatggtctc
     2161 gcggaccgac gactcgttca cggagcgggt tttcgcgctc gataaccggc ggatctacaa
     2221 gggtataccg gcggcggagc gcgaccttct tgaatcggat cgcgagccgg tctgaccggt
     2281 gctagccggc acgggccgtg cgattgcgtg caccgccgcc ttggcggctt caccggatcg
     2341 cggttagaat tttgaatgaa tcctcgacgt taacgtgagc gtcgcggcga gtcggttcca
     2401 accgcatcaa cgacgagaga tgtttccgat gaatgtgatc cgcagatcga cctggactat
     2461 actttttctg ggcaccgtca tcggctgcag cggccaggaa gaggcggtgc ctccccctga
     2521 atcgaagccg gaaattccgc ccgcgggcgc t
//
LOCUS       DATAQL010000727         4552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5418647, whole genome shotgun sequence.
ACCESSION   DATAQL010000727 DATAQL010000000
VERSION     DATAQL010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5418647"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            958..2331
                     /locus_tag="VHS97_12645"
     CDS             958..2331
                     /locus_tag="VHS97_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3449098.1"
                     /translation="MKPHSRLAVLVICSSVAALALSALAGCQKTAAQAEPPMPTVGVV
                     DSRRMNVPVVVTPNGTTRALEDVTIRARVRGFLTERHFVEGATVRKGQLLLVIDEEPY
                     KIALQSALARQAEAAASLRKAEESQMREVAAAQVELDRAQLVLAQIQERRTRTLLARH
                     ASSAEELDKTEADRRKWESQVAADVAQLRQSNADYEVAIASARAQVDAAKAAVHDAEL
                     NLGYCRMYAPLDGRIGEAKVKVGNLVGPDSSGGGAFSELATIQQLDPMGVDIRLSSRD
                     LDRTRHLIHGGLAVRLSRSGLLGDQEHPYEGKGYFIDNIIDETTSTFLAKARMPNPDG
                     MLLPGEYVKVRLVVDQLENAVVVPARSVMETDSGPVVHLVDSQGKVAVQRVEATQTYQ
                     GLRVIAKGLDSGVPVIVEGLQSIRPGIAVKTESAVSRLPATLDTNISSASPGALAPVP
                     PTGANRS"
     gene            2430..>4552
                     /locus_tag="VHS97_12650"
     CDS             2430..>4552
                     /locus_tag="VHS97_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449099.1"
                     /translation="MVDFFIRRPIFATVSAVLMLLIGGICAFLLPIAQYPQIAPPQVQ
                     VTTTYTGADASSVARTVTTPIEQQINGTKGLIYYSSDSTSNGVSNIVATFDVGFSQDL
                     AAVDIQNKVQTAQAQLPPEVKQYGVTIKKSSTDMVCVVNLISPDGRYDATFLDNYGQI
                     YVADVLKRIPGISDANVLGRKYAMRIWINPDRLANMRIAPTEVIQAVQQENVQAAAGK
                     IGGRPVPNGQDFELPITVKGRLEKAAEFDEIIVRRNNDGSIVRLADVARVELSSENFE
                     TASYIDGKPAGGILIYQYADANALSIVSQIRIEMDRLKKSFPEGLDYTIVYNTTEYVD
                     ENIREVQHTLLESFVLVMVVVFLFLQGFRATLIPMLAIPVSLVATFAAMAAFGFSLNS
                     LTLCGLVLAIGLVVDDAIIVVENVEKFLHRGLRPLAATRAAMAEITTPIVTITLVLAA
                     VFVPVAFMPGMTGRLYNQFAMTIVFSFGFSAFNSLTFSPAMARLFLKPKHGETRFFLF
                     RWFNAGLRWVENSYDAVLEFTAHHWWTIVAPSVILLGLTGWLVAARPKSFIPTEDQGY
                     LIVTVQTPDGTSSEATQRVLTRVESLCRGTEGIRHTVALEGLNVITSANQTNCGVVFL
                     PLTEWSLRSKPELRAVGLAASIQQKLSSEIRDASVMVLQPPPIRGLSSTGGFELMVED
                     RSGKGTDELQRVIDRFQDEARKRPEL"
BASE COUNT          934 a         1432 c         1350 g          836 t
ORIGIN      
        1 caatcggcca atggcccctg gcagagtcga aacgcgactg acggcggatt tgggttcacg
       61 gagccgaccc gcgagcgaaa atttctcgac ccacagcccg cgatgacgaa cgcgccctga
      121 ccagccaagt tcgcacgcga gtctgaagag ttacgtcaaa gaacgagtgg cactcctggg
      181 aaagtttcca gtgccaactc gccccgccag aagcggacag cgctcgggtg caagtcgaat
      241 attcttggag ctctatgccg atgaaatcct cccgcgtcca gtcgcgtccg gatgtcgaat
      301 ccgcacgaat atgcgcactc gtccgcccgt gacgtaagtc gcagtggagc gcctggacat
      361 aggaggtgtc tgccgattgc gcattggcaa attccggcaa ggttgaaaag taggctacac
      421 ctcaggggtc ggatggtgtc gaagcccgaa gggcgacgcc cccagatccg aggcttccgc
      481 gccgggggct cgctggctgt cgcccgctcg accccagcca ctccccgagg cgtaaccggc
      541 tctcgaacca tgcccagaga aataatgctg cctaacgcgg accaagggct aatgactgag
      601 gaccactgcc tcagtaagga atgaggaatg aggaaggagg accgaggaca aaggaccaat
      661 gaccaaggac caatgaccaa tgaccaatga ccaatgacca atgaccaatg accaatgacc
      721 aaggaccaag gaccaaggac caaggaccaa ggaccaatga ccaatgacca atgcctaggc
      781 cttgacttcg cgtcaatcgc aactattatg gttacgatct cgattcgcaa ccaacgccgt
      841 tacaaaatag tggctgcttt cggttgcgtt tgaatcggga attggcggct ttcacatcgg
      901 caagctgcgc cggcgtgagg ttgcggtccc agtcaaaagc tttgagggca ctaagtgatg
      961 aaaccgcatt ctcgcctggc cgttctcgtg atttgcagtt cggtggcggc actcgccctg
     1021 tctgcattgg cggggtgtca aaagacagcc gcccaggctg aaccccccat gccgaccgtg
     1081 ggcgtcgtcg actctcgtcg catgaatgtc cccgtcgtgg tcacgcctaa cggaaccaca
     1141 cgagccctag aggatgtgac gatccgggct cgcgtccgcg gctttttgac cgagcgccat
     1201 ttcgtggaag gtgccaccgt caggaagggg cagcttctgc tggtgatcga cgaagagccg
     1261 tacaagatcg ccctccagtc ggcccttgcc aggcaggcgg aggccgcggc cagcctcagg
     1321 aaggccgaag agtctcagat gcgcgaggtt gccgcggccc aggtcgagct cgaccgtgcg
     1381 cagttggtgc tggcgcagat ccaggagcga cgaacccgca cgctcttggc gcgccacgcc
     1441 agctcagccg aggaactgga caaaactgag gccgatcgcc gcaagtggga gtcgcaagtc
     1501 gcggccgacg tcgctcagct tcgccagtca aatgccgact acgaggtcgc gatcgcgtcg
     1561 gcacgggccc aggtcgatgc ggccaaggcc gccgttcacg atgccgagct caacctcggt
     1621 tactgccgca tgtacgcccc gcttgacggt cgcatcggcg aggccaaggt caaggtcggc
     1681 aacctggtcg ggcctgattc cagtggcggg ggtgccttct ccgagctggc cacgatccag
     1741 cagctcgatc cgatgggtgt cgacatccgc ctttcctcac gcgacctcga ccgcacaagg
     1801 catttgatcc acggcggact ggccgttcgc ttgagccgtt ctggcctgct gggcgatcag
     1861 gaacacccct acgagggaaa aggctacttc atcgacaaca tcatcgatga gacgacttcc
     1921 acgttcctgg ccaaggcccg gatgcccaac cccgacggga tgctcctgcc gggcgagtac
     1981 gtcaaagtgc ggctcgtcgt ggaccagctc gaaaatgccg ttgtggtacc cgcgcgatct
     2041 gtcatggaga ccgattccgg gccggtcgtg catctggtgg atagccaagg caaagtcgcc
     2101 gtccagcgcg tcgaggccac gcagacgtac caggggctgc gcgtcatcgc caaggggctc
     2161 gactcaggcg tgcctgtgat cgtcgaaggg ctgcaatcga tccgccccgg catcgcggtc
     2221 aagaccgaaa gcgcggtctc tcggctgccc gccaccctgg acacaaacat ctcctcggcc
     2281 tcccccggcg cattggcacc agtgcccccc accggcgcaa accggtcctg agcagagtct
     2341 tctagaccca gcgccaatca attagcggca acacattagt gcatagataa tcgaatcccc
     2401 gcggatccca acggaaagcc cgaaccaaaa tggtcgactt cttcatccgc cggccgatat
     2461 tcgccacggt ctccgccgtg ttgatgctct tgatcggtgg catctgcgcc ttcctgttgc
     2521 cgatcgcgca ataccctcag atcgcacctc cgcaggtgca ggtcacgaca acctacaccg
     2581 gtgcggatgc gtcgagcgtt gcccggaccg tcactacgcc gatcgagcaa cagatcaatg
     2641 gcacaaaagg gctgatctac tacagctctg acagcacctc gaacggcgtg tcaaatatcg
     2701 tggcgacatt cgacgtgggc ttctcgcaag acctggccgc ggtcgacatt cagaacaagg
     2761 tccagaccgc ccaggcacag ttgccccccg aggtcaagca gtacggcgtg accatcaaaa
     2821 agtcgtcgac cgacatggtg tgcgtcgtca acctgatctc gcccgacggc cgctatgacg
     2881 ccacgtttct cgacaattat ggccagatct atgtcgccga cgttttgaag cggatcccgg
     2941 gcatcagcga cgcgaatgtg ctgggccgca agtatgcgat gcgaatctgg atcaatcccg
     3001 accgcctggc aaacatgcgc atcgcgccca ccgaggtgat tcaagcggta cagcaagaaa
     3061 acgtgcaggc tgccgccggc aagatcggcg gccggccagt tcccaacgga caggacttcg
     3121 agttgccgat caccgtcaag gggcggctgg agaaggcggc tgagttcgac gagatcatcg
     3181 tgcggcgcaa caacgatgga tcgatcgtgc gcttggccga cgtggcccgg gtcgagctat
     3241 cgtccgaaaa cttcgagacc gccagctaca tcgacggcaa gccggccggg gggatcctga
     3301 tctatcaata cgccgatgcc aatgctcttt ccatcgtcag ccagatccgc atcgagatgg
     3361 accgcctcaa aaagagcttt cccgaaggtc tcgactacac gatcgtttac aacacgaccg
     3421 agtacgttga cgaaaacatt cgcgaggtgc aacacaccct tctcgaatcg ttcgttctgg
     3481 tgatggtggt cgtcttcctg tttctccagg gctttcgcgc gacgctcatc ccgatgctgg
     3541 cgatcccggt ctcgctggtt gcgaccttcg cggcgatggc cgccttcggc ttctcgctca
     3601 actcgctgac gctctgcggc ctcgtcctgg cgatcggcct ggtggtcgac gatgcgatca
     3661 tcgtcgtcga gaacgtcgag aagttcctgc accgaggtct gcgcccgctc gcagccacgc
     3721 gcgccgccat ggccgagatc accacgccga tcgtgacgat caccctggtg ctggcggccg
     3781 tgtttgtgcc ggtcgccttc atgccgggga tgaccgggcg gctgtacaac cagttcgcca
     3841 tgacgatcgt cttctcgttt ggcttctcgg cgttcaattc gctcacattc agtccggcga
     3901 tggcgaggct gttcttgaaa ccaaagcatg gggaaaccag gtttttcctg ttccgttggt
     3961 tcaacgccgg cctccgctgg gtcgaaaact cgtacgacgc ggtgctcgag ttcactgccc
     4021 atcattggtg gacgatcgtc gcaccttcgg taatcttatt ggggctaacc ggctggctgg
     4081 tcgcggcccg gcccaagtca ttcataccga cggaagacca gggttacctg attgtgaccg
     4141 tccagacgcc cgacggcacc agcagtgagg cgacacagcg cgtgttgacg cgtgtcgagt
     4201 cgctgtgccg gggtaccgag gggatcaggc acacggtcgc cctcgaaggg ttgaacgtca
     4261 tcacctcggc caaccagaca aattgcggcg tggtcttcct gccgctcacg gagtggtccc
     4321 ttcggtccaa gcctgagctg cgcgctgtcg gtctggcggc ctcaatccag caaaagctct
     4381 caagcgaaat tcgcgacgcc agtgtgatgg tccttcagcc tccgccgatc cgcggtctga
     4441 gctcgaccgg cggtttcgag ttgatggtgg aagaccgatc gggtaaagga actgatgagc
     4501 tccagcgcgt catcgaccgg tttcaagacg aagcccgcaa gcgtcccgaa ct
//
LOCUS       DATAQL010000728         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2975528, whole genome shotgun sequence.
ACCESSION   DATAQL010000728 DATAQL010000000
VERSION     DATAQL010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975528"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VHS97_12655"
     CDS             complement(<1..220)
                     /locus_tag="VHS97_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449100.1"
                     /translation="MIDPGSRTGRLGDARQHNDLAGAMVFITDHEESEQQNVGGGGDL
                     PSTWAGNCSRAWAFALLRVRHPGARHPPD"
     gene            410..1756
                     /locus_tag="VHS97_12660"
     CDS             410..1756
                     /locus_tag="VHS97_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449101.1"
                     /translation="MLVFGPVANGQESVIDTRDAFGILRTITIDEKPLDLSNPFFQSL
                     GTNGRSCASCHVASSAWTITPAELKRRFRSTDGLDPIFRTNDGSNSPNADVSTVRARH
                     KAFSMLLTKGVIRIGLPIPSGAEFALVRVDDPYCFASASELSLFRRPIPATNFRFLTA
                     VMWDGRESFAPLGTTPILASASDDQNAMALFNDLKHQANDATLTHAQGAVPLADDTAQ
                     AIVEFELNLATAQQRHDGAGELDVRGAQGGPAFVAIQPFYVTINDVLGADHFGNPFEP
                     DVMTLFSAWACSNNDRRAAIARGAKLFGTKSIAIKGVGGLNDDLGLPTVSGTCTTCHD
                     SPNIGNHSVALPIDIGVSDGSRRTPDMPLYTLQNLQTGETRTTTDPGRALLTGHWKDI
                     GKFKGPVLRGLAARPPYFHNGLAADLGAVVDFYNTRFAISFTEHEKADLIAFLSAL"
     gene            1910..2395
                     /locus_tag="VHS97_12665"
     CDS             1910..2395
                     /locus_tag="VHS97_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449102.1"
                     /translation="MSARKLASAKRVQEKKQKRLQKLRRKQGGSSFGSSRLSDGPLGL
                     ESMSDVLKAFVEPWMEMAETDEARHKLLTLAVAAWNAALLPDHKRKPFIDDLASAGGI
                     ALADRASFYSIIQEMIDRKQAYFARITRSIFSFDVQKTGDRKYFIHVVSTLPLPPAGK
                     D"
     gene            complement(2418..2807)
                     /locus_tag="VHS97_12670"
     CDS             complement(2418..2807)
                     /locus_tag="VHS97_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449103.1"
                     /translation="MRHAWFLEFCWCRLLGVILAGAMVFAGAGCHQHYYYYGDACAPG
                     APVPSTVRTGPVCDVPAQSVEGGTKLTSGSTRATVVSGGSPSNSSRVVVSEPSEPSRV
                     SWRRSDPDGSLPTTSVQGTINDSSVIR"
     gene            complement(3135..>3445)
                     /locus_tag="VHS97_12675"
     CDS             complement(3135..>3445)
                     /locus_tag="VHS97_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX3449104.1"
                     /translation="SAGYTVMRLPLAVAGLFENWLEQHFPERKDKVLDRIRATKGGRL
                     NDSRFGVRMSGEGNAAEMIGQIFRTSCRRAGLNRAPWPVNNSAFRRPKPTSGQLTLFE
                     "
BASE COUNT          697 a         1089 c         1020 g          639 t
ORIGIN      
        1 gatcgggtgg gtgtcgagcg cccggatgac gcactctgag cagcgcaaat gcccaagctc
       61 gcgagcaatt cccggcccag gttgagggaa ggtcgccccc tccgccgaca ttttgctgtt
      121 cgctttcctc atggtccgtg atgaacacca tggctcccgc aagatcgttg tgctggcggg
      181 catctcccag acggccagtc cgcgaacccg ggtcgatcat tttctcgtgg tcacgcggat
      241 cctggatcgc tttatgcgga aaggaactgc gatgaggttc actcaatcac tcttcagtcg
      301 aagactgcag cgaccagcgt gtctgtccgc gccatgcggg ccgataccct cccactgttc
      361 gggtcagagc agccgttccg tcgggggctg tgcatcggct atcgctctgt tgctggtctt
      421 cgggccggtc gcgaacggcc aggagagtgt aatcgacacg cgcgacgcgt ttggcatact
      481 ccgtacgatc acgatcgatg agaagccgct ggacctctcg aacccgttct tccagagcct
      541 tggcacaaac gggaggtcct gcgcttcctg ccacgtggcg tcgtcggcct ggacgatcac
      601 gcctgccgaa ttgaagaggc ggttccgatc gacagacggt ctggacccca tcttccgtac
      661 caacgacggc tcgaattcgc cgaacgcgga cgtctccacg gtcagggccc ggcataaggc
      721 gttcagcatg ctactcacca agggagtgat ccgtattggc ctgccgatcc cgtccggtgc
      781 ggaattcgcg ctcgtgcgcg tcgacgaccc ctactgcttc gcgagcgctt ccgagctctc
      841 gctgttccgg cggccgatcc ccgcgacaaa cttccgcttc ctcaccgccg tcatgtggga
      901 cggccgcgag tcgtttgctc cgctgggcac gacccccatc ctggcgagcg cgtcagacga
      961 tcagaatgcg atggccttgt tcaacgacct gaaacatcag gccaacgatg cgaccctcac
     1021 acatgcccag ggcgccgtgc ctttggcgga cgacacggcg caagccatcg tggagttcga
     1081 gctgaacctc gcgacggccc agcagaggca cgatggcgcc ggcgagctcg acgtcagggg
     1141 cgcccaggga gggccggcct tcgttgcgat ccagcccttc tacgtcacaa tcaacgacgt
     1201 cctgggcgcg gatcatttcg gcaacccgtt cgagccggac gtgatgacgc tgttcagcgc
     1261 ctgggcgtgc tctaacaacg atcgtcgggc cgcgatcgcg cgaggcgcca agctcttcgg
     1321 cacgaagtcg attgccatca aaggagtcgg agggctgaat gatgatttag gcctgccgac
     1381 agtctcgggc acctgcacga cctgccatga tagccccaat atcggcaacc attcggtcgc
     1441 cttgccgatc gacatcggcg tgagcgacgg gtcgcggcgc acgccggata tgccgctcta
     1501 caccctccag aatctgcaaa cgggcgagac ccgcacgacc accgatcctg gccgagccct
     1561 gctcacaggc cactggaagg acatcggcaa gttcaagggc cccgtgttgc gcggtctcgc
     1621 cgcgcgaccg ccgtacttcc acaacggact cgccgctgac ttaggcgcgg tggtggattt
     1681 ctacaacacc cgatttgcga tcagcttcac cgagcatgag aaggccgatc tgattgcgtt
     1741 tttgagcgcc ctgtaagccc ttgcttgcgc gcgatgcgta ttgcgtcttc gttaaaccct
     1801 gcggtaaact tcggcgcacg aaccggaagt cggcaatcgg gcagcaatgc taccgactgg
     1861 gtcgattgcc gaaaccgaca cggaaacgag atcacacgga ggagtgacca tgtctgcccg
     1921 aaaattggcg agcgccaagc gcgttcagga aaaaaagcag aaacgactgc aaaagctcag
     1981 aaggaagcag ggcggaagct ctttcggttc gtcgcgtcta tccgacgggc cactcgggct
     2041 ggaaagcatg tctgatgttc tgaaggcttt tgtcgagcct tggatggaga tggccgagac
     2101 ggacgaggcc cgccacaagc tgttgacctt ggctgtggcc gcgtggaacg ccgcacttct
     2161 acccgatcat aagcgaaaac cattcatcga cgatctcgcc tctgccggcg ggatcgccct
     2221 ggccgaccga gcgtccttct attcgattat ccaggagatg atcgatcgca agcaggccta
     2281 tttcgcaaga attacgcgat ccatcttcag ttttgacgtg caaaaaactg gcgacaggaa
     2341 atactttatc cacgtggttt cgacgctgcc tctcccgcca gccggcaaag attgacgctg
     2401 cgacggtctc acgctcgtca gcgaatgacg gacgagtcgt tgatcgttcc ctgcacgctg
     2461 gtcgtgggca ggctgccgtc cggatcagat cggcgccagg aaacacgcga cggttcgctg
     2521 ggttcgctga cgaccacgcg cgacgaattg ctgggcgacc cgccgctgac gacggtcgcg
     2581 cgggtcgacc cactcgtcag cttggttcca ccttcgacag attgcgcggg aacatcgcac
     2641 acagggccag tccgcacggt gctcggcaca ggcgcacccg gtgcgcacgc atcgccgtag
     2701 taatagtagt gttggtggca gcccgcgccc gcgaacacca ttgcgccagc caggatgact
     2761 ccaagcagcc gacaccaaca aaattcgagg aaccaagcat gtcgcatggc gaatctcccg
     2821 tgccgcaatt cagcctccgc gtcagagtgc cgccaagcga cggacgcgat cgaaaccggc
     2881 ttcggcaccc taaacctgga aagcgcggcc aaccacagcg ccgtatctcg atgattgcgt
     2941 cgaatcggac ggatgtgtgg acgcaacgaa gtaacccaag cccgacatcc gtgtcgaaac
     3001 tcaggagtgg gaactttaat aaaataccgc aatcgggcca accgcaattt ggcctgagag
     3061 aaaactagcc gcaaagcccg tccggccaag tgattggggg agatctacca tggggtgaca
     3121 aagtcctggc ggcgttattc gaagagagtg agttgcccac tcgtcggttt tggtcggcgg
     3181 aaagccgagt tattgacggg ccacggcgcg cggttcaacc ccgcccgacg acacgaggtg
     3241 cggaatattt ggccgatcat ctcggccgca tttccttcac ctgacatccg cacgccaaac
     3301 cgcgaatcgt tgagccttcc acctttcgtg gcgcgaattc ggtcgagcac cttatccttc
     3361 cgctccggga aatgttgctc cagccagttc tcgaagaggc ctgcaacggc cagcggcaac
     3421 cgcatgacgg tgtaaccggc cgacc
//
LOCUS       DATAQL010000729         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2366332, whole genome shotgun sequence.
ACCESSION   DATAQL010000729 DATAQL010000000
VERSION     DATAQL010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366332"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHS97_12680"
     CDS             <1..691
                     /locus_tag="VHS97_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3449105.1"
                     /translation="WSAKALHRVILTSSVYRQSSTPQLKATRVDPDNRLLARFPLRRL
                     DADAIRDAMLVASGELDDRQGGPYVPTRRTESGEIVADESSAGATRRSVYLQQRRTQT
                     ASLLDVFDAPSIVTTCTRRLASTIPLQSLSLLNAGFVVARAQKLAERLERDCKCVAAG
                     QAGDDARINRAFLLTTGRAPDRDERDAARRFLDSQPSRYPGRAEADAQGQAWTDFCQM
                     LLASNAFLYVE"
     gene            704..>1797
                     /locus_tag="VHS97_12685"
     CDS             704..>1797
                     /locus_tag="VHS97_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3449106.1"
                     /translation="MIPVPPMGQSLFPLNRRTFLGRYAGAIGTLALSHLLEQAVTRAG
                     ETVNPATTANPRPRAKSVICLFQHGGPSQMDLFDPKPALARHHGKPYPGQLEIHFDKK
                     AGNLLASPFRFRPHGQSGMVLSEIVPHMSQIVDDLTLVRSMTTESVDHESALRLIHTG
                     KVLAGRPTWGSWVVYALGSEDQNLPAYVVLSDPGGLPVDGVRNWSSGWLPAIDQGTPF
                     RSGDAPVLNLKTPPGITAEAREYQLRFLDELNRAHGKRHPGDTELEARIANFEIAAAM
                     QTAVPEALDLSRESEATRRMYGLDNPATREYGSRCLIARRLVERGVRFVQLFLSGQPW
                     DTHAKNAETLKSLCARTDQPSAAMVMDLKQR"
BASE COUNT          302 a          658 c          541 g          296 t
ORIGIN      
        1 ctggagcgcc aaggcattgc accgcgtgat cctgacttcg tcggtctatc gacagtcgag
       61 cacgccgcaa ttgaaagcca cgcgagtcga tcccgacaac cgcctgctgg cgcggtttcc
      121 gctgcgccgg ctcgacgccg acgcaatccg tgacgcgatg ctcgtcgcct ccggcgagct
      181 cgatgaccgc cagggggggc cgtatgttcc gacccgtcgc actgagtccg gcgaaatcgt
      241 cgccgatgaa tcgtcggccg gcgcgactcg ccgctcggtc tatctccagc agcgccggac
      301 ccagaccgcc agcctgctcg acgtgttcga cgccccctcg atcgtcacca cctgcacgcg
      361 tcggctcgcg tccacgatcc cgctccaatc gctcagcttg ctcaatgccg gcttcgtcgt
      421 ggcccgcgcg caaaagctcg ccgagcggct cgagcgcgat tgcaaatgcg tcgccgcagg
      481 ccaggccggc gacgacgccc gcatcaaccg ggccttccta ctcaccaccg gccgcgcgcc
      541 ggaccgggac gagcgcgatg cagcccggcg gttcctcgat tctcagccct cccggtaccc
      601 cggacgggct gaagccgacg cccaaggtca ggcctggacc gatttctgcc agatgctcct
      661 cgccagcaat gcattcctct atgtagaatg aatgactatg tccatgatcc cagtcccacc
      721 gatgggtcaa tccctgttcc ctctcaatcg ccgcaccttc ctgggccggt acgccggcgc
      781 gatcgggacg ctcgccctct ctcacctgct cgagcaggcg gtgacccgcg cgggggagac
      841 ggtgaacccg gcgaccacgg ccaatcccag gccccgggcc aaatctgtaa tctgcctgtt
      901 ccagcacggc gggcccagcc agatggacct gttcgacccc aagcccgcgc tcgccagaca
      961 ccacggcaag ccgtaccccg gtcagctcga gatccacttt gacaagaagg cgggcaacct
     1021 gctggcctcg cccttccggt tccgcccgca cggccagtcg ggtatggtgc tgagcgagat
     1081 cgttcctcac atgtcccaga tcgtcgacga cctgaccttg gtgcgctcga tgactaccga
     1141 gtcggttgat cacgagtccg ccctgcgttt gatccatacc ggcaaggtgt tggccggccg
     1201 gccgacttgg ggctcgtggg tcgtctacgc cttgggctcc gaggaccaga acctaccggc
     1261 ctacgtggtc ttgtccgacc ctggcggatt gccggtcgac ggcgttcgca actggtccag
     1321 cggctggctg cccgcgatcg accagggaac gccgttccgt tcgggggacg cgccggtgct
     1381 caatttgaag accccgcctg gcatcacggc cgaggctcgc gaataccagc tccgcttcct
     1441 cgacgagctg aaccgcgcgc acgggaagcg gcatcctggc gacaccgagc tcgaagcacg
     1501 gatcgccaac ttcgagatcg cggcggcgat gcagacggca gttccggagg cgctcgacct
     1561 ctcccgggaa tcagaggcga cccggcggat gtacggcctg gacaacccag ccacccgcga
     1621 atacggctcc cgctgcctga tcgcccgccg cctggtagag cgcggcgtgc ggttcgttca
     1681 attgttcctg agcggtcagc cctgggacac gcacgccaag aatgccgaga ccctcaaatc
     1741 gctatgcgcc cgcaccgacc agcccagtgc tgccatggtc atggacctga aacagcg
//
LOCUS       DATAQL010000730         5440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_991502, whole genome shotgun sequence.
ACCESSION   DATAQL010000730 DATAQL010000000
VERSION     DATAQL010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991502"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VHS97_12690"
     CDS             <1..672
                     /locus_tag="VHS97_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3449107.1"
                     /translation="DFSKIEAVKLELDPAEFSLRRVLGETLRALALRAHKKGLELVCQ
                     IEPDVPDALIGDAGRLRQILLNLAGNAIKFTDEGEVVVRIEAGPVVTEAEPSSAEGSP
                     VASQMLLFLVSDPGIGSPADKQQKIFQAFQQEDDSTTRRYGGTGLGLSIATRLVALMH
                     GEIGVQSEPGQGSRFEFTARFGVQPRQPDQTLVRPLVELQGLRVLVVDDNATNRQILE
                     GWFRG"
     gene            970..1290
                     /locus_tag="VHS97_12695"
     CDS             970..1290
                     /locus_tag="VHS97_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX3449108.1"
                     /translation="MGKKLYVGNLSYNVSSSDLEQLFSQFGSVQSAQVITDRDTGRGK
                     GFGFVEMGSESEANAAINGLNEHEHEGRRLTVNEAKPREARTTGISGGGSGRGPSSYG
                     GRRY"
     gene            complement(1369..3393)
                     /gene="ligA"
                     /locus_tag="VHS97_12700"
     CDS             complement(1369..3393)
                     /gene="ligA"
                     /locus_tag="VHS97_12700"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX3449109.1"
                     /translation="MARKEIEHEIAQLRAELNRHNYLYYVEAKPEISDREYDRMMSRL
                     TELEAEHPELVSPDSPTQRVGGQPLSGFRTVRHAVPMLSIDNTYSYDELREWDVRVRK
                     GLNRDEPVRYVVELKVDGVAVSLRYEQGKLTLGATRGDGERGDDITTNLRTVREIPLV
                     LHSCPVDVLEVRGEVYMTNAELLRLNELRRAAEEVPFANPRNSTAGSLKMLDSRIVAQ
                     RRLRFISHGLGESSELGESSYFAIVQLMKKWGIPVSPQTRCYDSIDEVIEHAQRWNQE
                     RNTLEFQTDGLVVKVDDLNQRARLGSRSKSPRWTIAFKYEAEQAITRLLGIHVQVGKT
                     GKITPVAKLEPVQLAGTTVKRASLHNADELERKDIMIGDAVVIQKAGEIIPQVVRVET
                     DARKGTETRFDFPKSCPSCGAPVERSGDEVDYYCTNPPSRCPDQLKEWLRWYAHRDAM
                     DIDGLGEKLIDQLVDKKLVRSLADLYRLDASTLAGLERMGKKSAENLVQALEASKHRT
                     LDRLLTGLVIRHVGTRMSEIIAARVKTLDELRSMSLAALAGTPEVGPIVAASIHDYFQ
                     DPENQALIDDLAAVGVSPVPLEPVEAKGGKLPFAGKTFVLTGTLPKRSRAEAEKLIKE
                     RGGKISGSVSKVTSYVLAGEEAGSKLEKAKQLGITVLDEPEFERLADIGE"
     gene            complement(3545..3844)
                     /locus_tag="VHS97_12705"
     CDS             complement(3545..3844)
                     /locus_tag="VHS97_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449110.1"
                     /translation="MTASPGRREVFANPFFVVLMATSVVFVITVLGDLISPSVLERGP
                     VNPPPGAGSLAAAAWLDRNAPWVLAVEFAIMLLSGVLAMATDPWFSPRAKPKPPA"
     gene            complement(3846..4301)
                     /gene="ndk"
                     /locus_tag="VHS97_12710"
     CDS             complement(3846..4301)
                     /gene="ndk"
                     /locus_tag="VHS97_12710"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX3449111.1"
                     /translation="MHRTLIIFKPDCVQRRLVGSILTRFEAKGLRITALKMVRVERTI
                     AEKHYAEHHGKPFFEGLIEFITSSPVVVGVLTGNEAVTVVRGMLGATSGIVAAPGTVR
                     GDYSISKQNNLVHGSDSPESAAREIALWFRPEELVEYELAGSEWVLGGS"
     gene            complement(4605..5030)
                     /locus_tag="VHS97_12715"
     CDS             complement(4605..5030)
                     /locus_tag="VHS97_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX3449112.1"
                     /translation="MTADHAAPESLEIPRDILDAMIAHCVREAPLECCGILGGVAAPL
                     VTSIYPLRNESQSETRYNADPHDLINTQLEMRRCGEDILAIYHSHPRWDAIPSRTDLA
                     ENYYGEVPRIIVSLLGDSPDVRVWRLDPDRFEELRWGVV"
     gene            complement(5208..>5440)
                     /locus_tag="VHS97_12720"
     CDS             complement(5208..>5440)
                     /locus_tag="VHS97_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX3449113.1"
                     /translation="LNTSIIDDVLTVRDEDAFETARRLAREEGMLCGISCGAAAFAAI
                     QIASKIENAGKLVVVVLPDLGERYLSTPLFPE"
BASE COUNT         1034 a         1737 c         1635 g         1034 t
ORIGIN      
        1 gatttctcca agatcgaggc cgtaaagctc gagttggacc ccgccgagtt ctcgctccgg
       61 cgcgtgcttg gggagacctt gcgtgccctg gccctccgcg cccacaagaa agggctcgag
      121 ctcgtctgcc agatcgagcc cgacgtcccc gatgccctga tcggcgatgc cggacggctc
      181 cggcagatct tgctcaatct cgcaggcaac gcgatcaagt tcacagacga gggtgaagtc
      241 gtcgttcgca tcgaagcagg tccggtggtc acagaggctg agccaagctc ggcggagggc
      301 tcacccgtcg cgtcgcagat gctgcttttt ttggtcagcg atccaggcat tggcagcccc
      361 gccgacaagc agcagaaaat ctttcaagcg ttccagcagg aggacgactc gaccacgcgc
      421 cgttatggtg ggacggggct gggtctctcg atcgcgacgc ggctggttgc cctgatgcat
      481 ggcgagatcg gcgtccagag tgagcccggc cagggcagta ggtttgagtt cacggcgcga
      541 ttcggcgtgc agccgcgcca gcccgaccag acgctcgtgc gcccgctggt cgagctccag
      601 gggctccgcg tgttggtcgt ggacgacaac gcgaccaacc gccagatcct cgagggctgg
      661 ttccgcggct gacggaccga accaacgatg gctgccgacg gccttgagga ttagccaccg
      721 cgcgaaatca agagcgtcga gtatctgaac gcatggctcg attcacgatc gccctttaca
      781 tcgagccgcg actcggtaca atgcggtacg gttggaaacc gaaacgagtc cgcgaatcga
      841 tcccttcgga atctgttcac gtctatctct ctcgtcttcc tgcctgctgg accgtcatgt
      901 cggtcccttt cacacccgac ccggaaagca aacgaagggt cgtcgcacac tcgttcagga
      961 gttttcacag tgggcaagaa gctatacgtc ggcaacctca gctacaacgt cagcagttcc
     1021 gacctcgagc agttgttttc tcagttcggc agcgttcaga gcgctcaggt catcaccgat
     1081 cgcgacaccg gccggggcaa gggtttcggt ttcgtcgaga tgggcagcga gtccgaggcc
     1141 aacgcggcta tcaatgggct caatgagcac gagcacgaag gccgtcgcct caccgtaaac
     1201 gaagccaagc cgcgcgaggc ccgcaccacg ggcatctcgg gtggcggcag cggtcgcggc
     1261 cccagctcgt acggtggccg ccgctactaa gaaacaccga tcacacaaac agacgccgag
     1321 gccgttcgca cggccttggc gttcttcatt tgcagcggca catcgaccct attcaccaat
     1381 atcggccagc cgctcgaact caggctcgtc aaggacggtt atgccgagtt gcttggcttt
     1441 ctcgagcttg ctgccggctt cttcacccgc caaaacgtag ctggtcacct tcgagactga
     1501 cccgctgatc tttccccctc gttccttgat cagtttctcg gcctcagccc gagatcgctt
     1561 cggcagtgta ccggtcaaga caaacgtctt gcccgcgaaa ggtagcttcc ctccctttgc
     1621 ttcgaccggc tccaagggta cggggctcac gccgacggcc gccagatcgt cgatcagtgc
     1681 ctggttttcc ggatcctgaa aatagtcgtg aatgctggcc gccacgatcg gtccgacttc
     1741 cggtgtcccc gcgagcgcgg caagcgacat cgagcggagc tcgtcaagcg tcttgacccg
     1801 ggccgcgatg atctcggaca tccgggtgcc aacgtggcga atcaccagtc cggtcaaaag
     1861 ccggtcgagg gtccggtgct tgctggcctc gagtgcctgc accaggtttt cggccgattt
     1921 cttgcccata cgctccagtc ccgccagggt cgacgcgtcg agacggtaca agtcggcgag
     1981 gctgcgcacc agctttttgt cgaccagttg atcaatcagt ttctccccca agccgtctat
     2041 gtccatggca tcgcggtggg cgtaccagcg caaccattct ttgagctggt ctgggcaacg
     2101 agagggggga ttggtgcagt aatagtcgac ctcgtcgcct gaccgctcga ccggggcacc
     2161 gcaactggga caggacttgg ggaaatcgaa gcgcgtctcg gtccccttgc gcgcgtcggt
     2221 ctcgacccgg acgacttgcg ggatgatctc gccggccttc tgaatgacga cggcgtcgcc
     2281 aatcataatg tctttgcgct cgagctcgtc ggcattgtgc agactggcac gtttgacggt
     2341 cgtgccggca agctgcacgg gttcgagctt ggcgacaggc gtaatcttgc ccgttttccc
     2401 gacctgcaca tggatcccga gcaaacgcgt gatcgcctgc tcggcctcgt acttgaaggc
     2461 gatcgtccac cgcgggctct tgctccgcga gcccagccgc gcgcgctgat tgagatcgtc
     2521 gaccttgacc accaggccgt cggtctggaa ttccaacgtg ttgcgttctt gattccaccg
     2581 ctgtgcgtgc tcgataacct cgtcgatcga gtcataacag cgcgtctggg ggctgaccgg
     2641 gatgccccat ttcttcatca actggacgat cgcgaaatac gacgactcgc ccagctcgct
     2701 cgactcaccc aggccgtgag agataaaccg cagccgccgc tgggcgacga ttctggaatc
     2761 gagcatcttg agcgagccgg ccgtcgaatt ccgggggttg gcaaacggga cctcttccgc
     2821 ggcccgccgc agctcgttca accgcagcaa ctcggcgttg gtcatgtaga cctcaccccg
     2881 aacttccagc acgtcgaccg ggcaactgtg cagtaccagg gggatctctc ggaccgtcct
     2941 cagattcgtc gtgatgtcgt cgccccgttc gccgtctcct cgggtcgctc ccagtgtgag
     3001 cttgccttgc tcataacgga gcgacacggc gacgccgtcg actttcagct cgacaacata
     3061 gcgcacgggc tcgtcgcgat tgagcccctt gcgcactcgg acatcccact cgcggagctc
     3121 gtcgtacgaa tacgtgttgt caatcgacag catcgggacc gcatgacgca cggttctgaa
     3181 acccgagagc ggctgaccgc cgacccgctg cgtggggcta tccgggctca cgagctcggg
     3241 gtgttcggcc tcgagctcgg tcagccggct catcatccgg tcgtattcgc ggtcgctgat
     3301 ctccggcttg gcttcgacgt agtaaagata attgtgccgg ttcagttccg cgcgaagctg
     3361 ggcaatttcg tgctcgatct ccttgcgcgc catggccggg cctcacgcgt gggtggctgg
     3421 ggtcgtcgcc atatgaccga cgaccccagt ggcaacgact gtgggctcgc cggcttgaag
     3481 ccggcttcga ccacagccac cggctgtccc ggcgggcgaa acccaatttc tcatcggctc
     3541 tacctcacgc gggcggtttc ggtttggccc gcggcgaaaa ccaggggtcg gtggccatcg
     3601 ccaggacgcc gctcaagagc atgatcgcga actcgacagc caaaacccac ggtgcgttgc
     3661 gatcgagcca ggcggccgcc gctagcgaac ccgctccagg aggcggattg acaggtcccc
     3721 gctcgagcac cgaggggctg atcaaatcgc cgagcacggt gatgacaaaa acgacgctgg
     3781 ttgccatcag cacgacgaaa aacggattgg cgaacacctc tcgtctgccg gggcttgctg
     3841 tcatcttacg atccgcccaa aacccactcg ctgccggcga gttcgtattc gaccagctcc
     3901 tccggccgga accagagcgc gatctcgcgc gcggccgact cggggctgtc gctaccgtgg
     3961 accaagttgt tttgcttact gatcgaatag tctccgcgga cggtacccgg agcagcgacg
     4021 atgccacttg tcgcccccag catcccccgc accaccgtga ccgcctcgtt gcctgtgagc
     4081 acccccacaa ccaccggcga actggtgata aactcgatca acccttcgaa aaatggcttg
     4141 ccatggtgct cggcataatg cttctcggcg atcgtccgct cgacgcggac cattttgagg
     4201 gccgtaattc gcaggccctt ggcctcgaat cgcgtcagga tcgagccgac gagtcggcgc
     4261 tgaacacagt cgggtttaaa aatgatcaat gttcggtgca tggaactcaa aggcttttcc
     4321 agattcggga tgagaaaaaa acgcacttca gtctagtgcc ggtgcgtcac tcttgcaacc
     4381 tcaagcctcc gaggatgatc cccagggcgt tgccctgggc tggcatgtgt ttggccctcc
     4441 gggccgaaat tcggaagaat aaacctcgac acctcgcccc aaaggggcat taacaaacta
     4501 gcccagggca acgccctggg ttgccaaggc gtaatccatg cgcagtgccc tgaaagggca
     4561 cgacaaggat gccggaaact gttggcgcgg ccgcagtccg cgggctagac gacgccccag
     4621 cggagctctt caaagcggtc cgggtcgaga cgccagacac gcacgtccgg gctatcgccc
     4681 aagagcgaga cgatgatccg gggcacctcg ccgtaatagt tttccgccag gtcggtccgg
     4741 ctggggatcg catcccagcg gggatgcgaa tggtaaatcg ccaggatatc ctcgccgcag
     4801 cgccgcatct cgagctgagt attgatcaaa tcatgcggat cggcgttgta acgtgtctca
     4861 ctctgagact cgttgcgcag cgggtaaatc gacgtgacga gcggagccgc gacacccccg
     4921 agaatgccgc aacattcgag cggtgcctcg cgcacgcaat gagcgatcat tgcgtcgaga
     4981 atatcgcgag gaatctcgag tgattcgggc gcggcgtggt cggccgtcat ggcatgctct
     5041 tagcctcgca aacggacgca gcggccccta aacagccttg cgtgattccg ggagggcgag
     5101 gctcggctcg actgagctcg ccgaagtccg tccgagccgg attgcgccgt ggctcgccgg
     5161 gaggctcgcc ctcccaggat cgtgcaacag catttaggtc acagcgacta ctcggggaag
     5221 agaggcgtcg agagatagcg ctcacccagg tcaggcagaa ccacgacgac cagcttaccg
     5281 gcgttttcta ttttggatgc gatctggatg gccgcaaaag ccgccgcgcc gcaggaaatg
     5341 ccgcagagca tcccctcctc acgagccagc cggcgggctg tctcgaaggc atcttcgtcg
     5401 cggaccgtca acacatcatc aataatagag gtgttgagca
//
LOCUS       DATAQL010000731         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5113070, whole genome shotgun sequence.
ACCESSION   DATAQL010000731 DATAQL010000000
VERSION     DATAQL010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5113070"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2973
                     /locus_tag="VHS97_12725"
     CDS             <1..2973
                     /locus_tag="VHS97_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX3449114.1"
                     /translation="GELARLQTKNVVVLLERARLAAKYNDAEALRDSVARLGELAPSW
                     PEKGRDIFRELDDVSKGNLRLAAMRVVFLRNVLVQAPSFRQDLSAVETPAGTVGEPIE
                     QFLRLTPPRPTPSPPDDALAFAIESLGASASAQGVFLLPDGEGTPALFVANGREVRRT
                     DGTGPVLPFPGGPTATISPHGVLAVDLNSDFRIDLVLAGAGGLRFFQQKPDGTFADVT
                     AATGLSPEVLGLNAFGAWVADIEMDGDLDIVLGVRDKGSTVLRNNNDGTFGIVQPFAG
                     TIDVRDFAWADLDQDGDPDAAMVDARGLLHVYRNERAGRFQARPGPEHLDNVGALEAA
                     DVNGDGIVDLVAVCTDGSVRRISDKDEGNAWEVAQLPLAPGASEGADRLFVADLDNNG
                     ALDLICSGPAGAWIALGGEAGIWRTVSSPPNLRISAVADLNADGLLDLAGMSADGRPA
                     RGLGRGTKGYHWQVIRPRGARTFGDGRINSFGIGGEAQLRAGMLVQKQVISGPILHFG
                     LGDQPTADYVRIFWPNGTVQGEFDTKADQTLVAEQRLKGSCPFLFAFDGTGMRFVTDF
                     IWRSPLGLRINAQDTAGVSQTEDWVKIAGDQLVASEGYYDLRITAELWETHFWDHMSL
                     MVVDHPRDTEIFVDERFARQPPPLAVHTTGPMHPIAYAWDDTGRDVTEIVRARDGRYL
                     DTFGRGAYQGVTRDHWVEAELGDDVPWDRPLRLIAHGWIHPTDSSINVAISQGHHAQP
                     QGLVLEVPAADGSWTVARSDLGFPAGKNKTILVDLDGIFRPGCRRHFRLRTNLEIFWD
                     SLTVATVLAESPLKLVRIAPASAELRPRGYSLMTQANESSPELPRYDSLTGTGQRWLD
                     LIGYYTRYGDVRDLVQTVDDRYVIANAGDELALRFTAPPPPPAGWMRDFVMVGDGWNK
                     DGDYNTAFSKTVLPLPSHKSSRYDTPPVTLEDDPVFRLHQEDWREYHTRYITPKEFQV
                     GVRPPLYGRPRRQAESNP"
     gene            2970..>3680
                     /locus_tag="VHS97_12730"
     CDS             2970..>3680
                     /locus_tag="VHS97_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3449115.1"
                     /translation="MKQYSRIVLILFFIALLIAPVVLNRFASPGVPTAVVGAGSKDAL
                     SRYGFRLQEVSKSAGIDFVHQSPKLDSKLDHIMPQIASMGASVSVADFDRDGWQDLYT
                     TDSSEGGRNRLYRNQGDGTFADVAEAMGLANVNQPGTGVSMGSIWGDCDNDGFEDLFL
                     YKWGRPELFHNDGGRGFTRVTEQAGLPAWVNAGCATWFDYDSDGHLDLFLAGYWNEQL
                     DLWNLKTTKMMPESFEYAQ"
BASE COUNT          656 a         1151 c         1165 g          708 t
ORIGIN      
        1 ggagaactcg cgcgattgca gaccaagaat gttgtcgtgt tgctcgagcg ggcgcggctg
       61 gccgccaagt acaatgatgc tgaagctctt cgagattcag tcgcacggct gggtgagctc
      121 gcgccctctt ggccggagaa ggggcgcgat atcttccgcg agctggatga cgtgtcaaag
      181 ggaaatctgc gcctggccgc gatgcgcgtg gttttcctcc gcaatgtgct ggtgcaggcc
      241 ccgtcattcc gccaggacct cagtgccgtc gagactccgg ccggcaccgt aggtgagccg
      301 atcgagcaat tcttgcggct caccccgcca agacccactc cttcgcctcc ggatgatgca
      361 ctcgcctttg cgatcgaatc gctcggcgca tccgcctccg cccaaggagt gttcctgctg
      421 ccggacgggg aagggacgcc cgcgctcttc gtcgcgaatg gccgcgaggt gcggcggact
      481 gatgggaccg ggcccgtgct cccgtttccc ggcggaccga cggcgacgat ctcaccgcac
      541 ggcgtgctgg ctgtggatct gaactccgat ttccgcatcg atcttgtcct cgccggcgcg
      601 ggcgggttgc gtttcttcca gcaaaagcca gatggcacgt tcgctgatgt gactgcggcc
      661 accggcctga gtccagaggt gctcggcctt aatgccttcg gtgcctgggt ggcggacatc
      721 gagatggacg gcgacctcga tatcgtgctc ggtgtccgcg acaagggctc gaccgtgctt
      781 cgcaataata atgacggcac gttcggaatc gtgcagccct ttgcgggaac catcgacgtg
      841 cgcgatttcg cctgggctga tctcgaccag gatggagatc cggatgccgc catggtcgat
      901 gcacgcggct tgctccacgt ctatcgcaac gagcgcgctg gtcggttcca ggcccggccg
      961 ggcccagaac acctcgataa tgttggtgcc ctcgaggccg ccgacgtgaa cggcgacggc
     1021 attgttgatc tcgtggcggt atgcaccgac ggctccgtgc gccggatctc agataaggat
     1081 gaagggaacg cctgggaagt cgcccagctc cctctcgcac cgggggcgtc agagggagcg
     1141 gaccggctct ttgtcgccga tctggacaat aacggggccc tcgacctgat ctgctcaggt
     1201 cccgccggag cttggattgc ccttgggggc gaagctggga tctggcgcac ggtaagctcc
     1261 ccgcccaacc tgcgcatctc ggccgtggcg gatttgaacg ccgacggcct gctcgatctg
     1321 gcgggcatgt cggcggacgg acgtccggcc cgaggactgg gtcgcggtac caagggctac
     1381 cattggcagg tcatccggcc ccggggcgcc cggaccttcg gcgatggccg gatcaactcg
     1441 ttcggaatcg gcggcgaagc ccagttgaga gcgggcatgc tcgtgcagaa gcaggtgatc
     1501 tccgggccga tcctccattt tggcctggga gaccagccga cggccgatta cgttcgcatc
     1561 ttctggccca atgggaccgt gcagggagag tttgacacca aggccgacca gactttggtc
     1621 gcagagcagc gcttgaaggg ctcgtgcccc ttcttattcg cgttcgacgg gaccgggatg
     1681 cgattcgtaa ccgacttcat ctggcgatcg ccgctgggac tgcgcatcaa cgcccaggac
     1741 actgccggcg tctcacagac ggaagactgg gtgaaaatcg ccggtgacca gctcgtcgcg
     1801 tccgagggtt attacgatct gcggataacc gcggagctgt gggagactca tttctgggac
     1861 cacatgtcgc tcatggttgt cgatcatccg cgggacaccg agatcttcgt ggacgaacgg
     1921 ttcgcccgcc agccgccgcc cttggccgta cacacaaccg gtccgatgca cccgatagcc
     1981 tacgcctggg acgacacggg ccgcgacgtc accgagattg ttcgggctcg tgacggcagg
     2041 tacctggata cgttcggccg tggcgcctat caaggggtga cgagagacca ctgggtcgag
     2101 gctgagctgg gtgacgacgt gccctgggac cggccgctgc ggctgattgc gcacggttgg
     2161 atccacccga ccgatagctc gatcaacgtc gccatcagcc agggtcatca cgcacagcca
     2221 cagggacttg tgctggaggt tccggccgcg gacgggtcgt ggacggtcgc acgctctgac
     2281 ctcggtttcc ccgcgggtaa aaacaagacc atcctcgttg atcttgacgg cattttccgt
     2341 cccggttgcc gccggcactt ccgcctccgg accaatctcg aaatcttctg ggattcgctc
     2401 accgtcgcga cggtcctggc cgaatctcct ctcaagttgg tgcggatagc ccccgcgtcg
     2461 gccgagctgc ggccgcgcgg ctattcactc atgacacaag caaacgaaag ctcacccgaa
     2521 ctacctcgtt acgactcgct cacaggcaca ggccagcgct ggctcgacct gatcggttac
     2581 tatacgcgct acggcgacgt tcgcgacttg gttcagacgg tcgacgatcg ctacgtgatc
     2641 gccaacgccg gggacgagct ggcgctccgt ttcaccgcac cgccaccgcc gccggctggg
     2701 tggatgcgcg acttcgtcat ggtcggcgat ggctggaaca aagacggtga ctacaatacc
     2761 gcgttttcga agacagtgct tcccctgccg tcgcacaaga gtagtcgcta cgatactccc
     2821 cccgtcacgc tcgaagacga cccggtgttt cgcctccatc aggaggattg gcgtgagtat
     2881 cacacgcgtt atattacccc aaaagagttt caggtcgggg tcaggccacc gctctatggt
     2941 cggccccggc ggcaagcgga atcgaatcca tgaagcaata tagccggatc gtgctcatcc
     3001 tcttttttat cgcgcttttg atcgcgccgg tggtgcttaa ccgctttgct tcgcctggtg
     3061 tgccaaccgc cgtcgtgggg gcaggatcca aagacgcctt gtcccgttac ggatttcgtc
     3121 tgcaagaggt gtcgaaatcc gcgggcatcg atttcgtgca ccaaagcccc aagctcgatt
     3181 ccaagctcga ccatatcatg ccgcagattg cgtcgatggg ggcctcggtc tccgtggcgg
     3241 actttgatcg agacggctgg caagacctgt atacgaccga cagcagcgag gggggccgaa
     3301 atcgcctcta tcgtaaccag ggggacggca cattcgccga cgtggccgag gcaatggggc
     3361 ttgccaacgt gaaccagccg ggaacgggcg tctcgatggg ctcgatctgg ggcgactgtg
     3421 acaacgacgg ctttgaagac ttgttcctct acaagtgggg gcgcccggag ctgtttcaca
     3481 acgacggcgg gcgcggtttc acgcgcgtca ccgagcaagc cggccttccc gcctgggtca
     3541 acgccggatg tgcgacctgg ttcgactacg acagcgacgg acacctcgat ctgtttctcg
     3601 ccggctactg gaatgaacag ctcgatctct ggaatctcaa gacaaccaag atgatgcccg
     3661 agagcttcga gtatgcccaa
//
LOCUS       DATAQL010000732         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5113079, whole genome shotgun sequence.
ACCESSION   DATAQL010000732 DATAQL010000000
VERSION     DATAQL010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5113079"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1606)
                     /locus_tag="VHS97_12735"
     CDS             complement(293..1606)
                     /locus_tag="VHS97_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3449116.1"
                     /translation="MSQTRRQTFCGSPDHALNRRGFLGTVAAGAAAFAADMTVLDVLQ
                     NPALAGELKRKDKRVILLWLAGGSSQLETWDPKPGAPTGGPFRSIPTAVPGIHISELM
                     PRMAQRMDQTCIIRSLNTKNGDHGGGARLMMHGRRDDASVRYPDLGAVLARELGQIDS
                     QVPDYVSFYSPTEGRTMAPGSAGFLGERYSPMHLTTGTIPENLGRLDEISELDHIERA
                     RLRQFVSKRFERGRSARALGSHNVAYERVRGLMASEGLFDISREPQKVRDRFGPTQFG
                     EQALVARRLVEAGVPFVRVARAWWDSHGQNFETHQEMVPELDLVMAALLDDLKERGLL
                     ESTLVITLAEFGRTPEINASLGRDHFATAWSATLSGAGIHRGSVYGKTDANGRSVVDG
                     EVGPAELFATIYQALGIDHQKQYMVGARPVPLTETGTEPIREVLS"
     gene            complement(1675..3309)
                     /locus_tag="VHS97_12740"
     CDS             complement(1675..3309)
                     /locus_tag="VHS97_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX3449117.1"
                     /translation="MRIVCPALLVAAMLTLIQSASRAAELLPSDRPIPAVVDHYIDAA
                     LHKEGIKATPAADGATLIRRLTLDLVGRIPTTAEWRAFAESTDPDKRPKLVDRLMASP
                     GFVRHQVNELDTMLMAGDKGSVREYLAAAIGDNRPWDQIFRELLLPDQTDKRTKPAAE
                     YLRRKVKDLDRLTAEVSSTFFGVNISCAQCHDHPLVRDWKQDHFYGMKSFLSRTFLNG
                     TENAGFVAERGFGSIRFKTTDDVERQARLMFLTGSRVDDPGDKEPSKEEQKKEKEALD
                     RAKKDKVPPPAPSFSARAKLVEVALRPGDREFFAKAIVNRIWHRLYGQGLVMPLDQMH
                     SANPASHPDLLAWLARDTAEHGYDLRRLIRGLVMSDAYARDSYWQAGEPPRASLFAVA
                     ILRPLTPAQLATSLRLATVDPSSLPPDFESGLFEKRIEGLETSGRHLAASFTTTSGDA
                     QIGVAEALFFSNSKQVEKELLGEGSDRLVGRLKQTSGTTELIDLAVRAVLSRPPVDDE
                     IRTLGTYLGERTDRPDDASEQLVWALLTSSEFRFNH"
     gene            3607..>4803
                     /locus_tag="VHS97_12745"
     CDS             3607..>4803
                     /locus_tag="VHS97_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3449118.1"
                     /translation="MNAFEDERLALPGPFPPRRGSWGGMAVAGFVFLGVGIVMVLYNA
                     CKIEVGTGQQAVLIRREGQELEPDMEVAPPPKDGKYYKGVQSGGPNNGVLTEGRYFYN
                     PYYWSWEIGPQFVVPSDKIGIRVALSGDDLPPGQILAEPGKKGILREVLKPGRYPYNP
                     YAEAIELHAPVTIPAGLRGVVTQLAGPTSNDPNVFLVKEGERGVQRKTLEPGTYYLNP
                     YEIRVSQVDCRSRRFNLGQDTEMSFLSADGFPITLDGVVEFRIEPDKVAEMFVKYNET
                     ANGDAIDEEIIAKIITPESRSLCRIGGSKLSGGQFISGQDREQFQTDLVKSLTQNCQK
                     QGIEILAVAITSIQPPADIAQPVQQREVAKQKLAQFRREKLQQLSEAKLKVEVVLAQQ
                     KKRVVEA"
BASE COUNT          902 a         1437 c         1540 g          924 t
ORIGIN      
        1 ttggtctggt ccagatcgat ctcgtagacc gtaaagtcag agccgccgca gaaaactctg
       61 ctcgtgccag gttttcgcgc cagggcaaac acgatatcgc ggcgtgacga ttccttgaca
      121 accttgatgg tctcagggcg ggacgcgcgc atcagtctca cctcggcggt taagggcaat
      181 accgttcaag tagtagagag ttgctggtgc ttctgaataa ataatcccct ccccccttgt
      241 agggaagggc caaggtggag ggctttcctt taaacgaccc gtggcctgcg ggctagctga
      301 ggacttctcg aatcggttca gtgccggttt cggtcagagg cacagggcgc gcgccgacca
      361 tgtactgttt ctgatgatcg atgcccagtg cctggtagat ggttgcgaac agctcggcgg
      421 gaccgacctc gccgtccacg acagaccggc cgttcgcgtc ggtcttgccg tagaccgagc
      481 cgcggtggat tcccgcgccg gagagcgtag cgctccaggc ggtggcgaaa tggtctcggc
      541 ccaggctcgc gttaatctcg ggagtgcggc caaattcggc cagtgtgatc accagcgtgc
      601 tctccaagag gccgcgttcc ttgaggtcgt cgagcaaggc cgccatcacc agatcgagct
      661 cgggaaccat ctcttgatgg gtctcgaaat tctggccgtg gctgtcccac caggcccggg
      721 ccacgcgcac gaacgggacc cctgcttcca ccagcctgcg ggcgactaag gcctgctcgc
      781 caaactgcgt cggcccgaag cggtcgcgaa ccttttgcgg ctcgcgcgag atgtcaaaca
      841 ggccctcgct ggccatcagc cctcgtaccc gttcgtaggc cacattgtgg ctgcccaggg
      901 cacgggccga acgcccgcgc tcgaacctct tgctcacaaa ctgacgcaga cgtgctcgct
      961 cgatatggtc gagctccgag atctcgtcca agcggccgag attctccggg attgtgcccg
     1021 tggtcaggtg catgggcgag tagcgctcgc ccaggaatcc ggcggacccc ggggccatcg
     1081 tacgtccttc agttggcgaa taaaacgaga cgtagtctgg tacctggcta tcaatctggc
     1141 ccagctcgcg cgccagcacc gcccccagat ctgggtaacg cacgcttgcg tcgtcgcggc
     1201 ggccgtgcat catcagccgg gcgcctccgc cgtggtcgcc attcttggtg ttgagcgagc
     1261 gaataataca cgtctggtcc atgcgctggg ccatccgagg catcagctcg gaaatgtgga
     1321 ttccgggcac agccgtcggg atcgatcgga acggaccgcc ggtcggagca cccggcttgg
     1381 ggtcccaagt ctcgagctgg ctcgaacctc ctgccagcca gaggagaata acccgcttgt
     1441 ctttccgctt aagctccccc gccagcgccg ggttttgaag cacgtcgaga acggtcatgt
     1501 ccgccgcgaa agccgccgca cccgccgcaa ccgtaccgag aaagccacgg cggttgaggg
     1561 catggtcggg cgacccgcaa aacgtctggc gtcgagtctg ggacatcgaa ttcgctcccg
     1621 cgtgttcaaa cttggtagaa aggtggggat ggattgagga gtgatgatat ctcgtcaatg
     1681 gttgaatcgg aattctgaac ttgtcagcag cgcccagacg agctgttcgc tggcgtcgtc
     1741 ggggcggtcg gtgcgctcgc ccaagtaggt gcctagcgtg cggatctcat cgtcgaccgg
     1801 gggccgggag aggaccgccc gcacggccag gtcgatcagc tcggtggttc cggaggtctg
     1861 tttcagacgc ccgaccagac ggtcggaccc ttctcccaag agctctttct cgacctgctt
     1921 gctgttactg aagaacaagg cctcggccac gcctatctgc gcgtcgccac tggtcgtggt
     1981 gaacgatgcc gccaggtggc gaccgctcgt ctccaggcct tcgattcgct tttcaaataa
     2041 tccggactca aagtccggtg gcagactgga tggatcgacg gtcgccagcc gcagcgacgt
     2101 cgccagttgc gcgggcgtca ggggacgcaa gattgccacg gcgaaaagcg acgcccgggg
     2161 cggctcaccc gcttgccagt aagagtcgcg ggcgtaggcg tcgctcatga ccagcccgcg
     2221 tatcaggcga cgcaggtcgt agccatgttc ggccgtgtcg cgggcgagcc atgcaaggag
     2281 gtcggggtgg ctggccgggt tcgccgaatg catctggtcg agcggcatca ccaatccctg
     2341 gccatacagt cggtgccaga tgcgattgac gatcgccttg gcgaaaaact cgcgatcgcc
     2401 aggccggagg gccacctcca ccagtttcgc ccgggcgctg aagctcggcg ccggtggagg
     2461 aaccttatct ttcttcgctc ggtcgagggc ctctttttct tttttctgtt cttccttcga
     2521 cggctctttg tcgcccgggt cgtcgacccg gctccctgtc aagaacatca gccgggcctg
     2581 gcgttcgacg tcgtcggtgg tcttgaagcg gatcgagccg aagccacgct cggccacgaa
     2641 acctgcgttc tcggtgccgt tcaagaatgt gcggctcagg aacgacttca tcccgtagaa
     2701 atggtcctgc ttccagtcac gcacgagcgg gtggtcgtgg cactgggcgc agctgatgtt
     2761 gacaccgaaa aaagtcgagc tgacctcggc ggtgagccga tcgagatcct tgaccttacg
     2821 ccggagatat tcggcggccg gcttcgttcg tttgtcggtc tggtctggta gcaagagctc
     2881 ccggaagatc tggtcccacg ggcggttgtc gccaatggcc gccgcgagat attcgcgcac
     2941 gcttccctta tcgccggcca tgagcatcgt gtcgagctcg ttcacctggt gacgaacaaa
     3001 gcccggggac gccatcagcc ggtcgaccag cttgggacgt ttgtcgggat cggtcgattc
     3061 ggcgaacgcg cgccattcgg cggtggtcgg aatccgcccg acaagatcga gagtcaaccg
     3121 gcggatcagg gtggcaccat cagccgcggg cgtcgccttg atgccttcct tatgaagtgc
     3181 cgcatcgatg taatggtcaa ccacggcggg gatcggacga tcggagggaa gcagttccgc
     3241 ggcccggctt gcagactgga tcaaggtcag catcgcagcc accaagaggg ccgggcaaac
     3301 aatcctcatg ctgggttcct cgtgctcagg atgaatggga tcgcaaactg ccgcgtgctc
     3361 gtggggcgag ccggaacagt ccctggctgg cgctactatt ttcatcggat tgcgggataa
     3421 caattgttga atatacgccg ttttgctggc ggaggctata gacttgtcat gctagaccaa
     3481 aaatagcgct tgcggcctta gcgggtcacg cccgattagc gggaataatg ggggcggtgc
     3541 ggcgaagcta ctcttgattt gttcagagtt tcggtttggc gtttgggtcg tttgaggagt
     3601 cagcacatga acgcgttcga agacgagcga ctggcgttgc ccggcccctt cccgccgcgg
     3661 cgcgggtcgt ggggtgggat ggccgtcgct ggtttcgtct ttctgggcgt tgggatcgtg
     3721 atggtgctct acaacgcctg caagatcgag gttggaaccg gccagcaggc ggtcttgatt
     3781 cgacgggagg ggcaagagct cgaaccagat atggaagtcg ctccgccgcc gaaggacggt
     3841 aagtactata agggcgtgca gtcgggcgga ccgaacaacg gtgtcttgac cgagggccgt
     3901 tacttttaca acccctacta ctggtcgtgg gagatcggcc cgcagttcgt cgtgccgagc
     3961 gacaagatcg gtatccgggt cgccctctcc ggtgacgacc tgccgccggg tcaaatcctg
     4021 gccgagcccg gaaagaaggg gatcctccgc gaggtcttga agccaggccg atatccgtat
     4081 aacccgtacg ccgaagccat cgagctgcac gccccagtga caatccccgc gggattacga
     4141 ggagtggtga cgcagctggc agggccgacg tccaacgatc ccaatgtctt cctggtcaag
     4201 gagggcgagc gcggagtgca gcgcaagacg ctcgaaccgg gaacgtatta tctgaatccc
     4261 tacgagatcc gcgtaagtca ggtcgattgc cggtcgcgcc ggttcaacct ggggcaggac
     4321 accgagatga gcttcctgtc ggcagatggc tttccgatca cgctcgacgg cgtggtcgag
     4381 tttcgcatcg agcccgataa ggtcgccgag atgttcgtca aatacaacga aactgccaac
     4441 ggcgacgcga tcgacgagga gatcatcgcc aagatcatca cgcccgagag tcgatcgcta
     4501 tgccggattg gcggctccaa gctctcgggc ggacagttca tcagcggcca ggaccgcgag
     4561 cagtttcaga ccgacctggt caagagcctg acccaaaatt gccagaaaca ggggatcgag
     4621 atcctggccg tcgccatcac ctcgattcag ccgccggccg acattgcgca gccggtacaa
     4681 cagcgcgagg tggccaagca aaagctcgcg cagttccgcc gtgaaaagct gcagcagctt
     4741 tctgaggcga aattgaaggt cgaggtcgtg ctggcccagc agaagaaacg cgtggtcgag
     4801 gcc
//
LOCUS       DATAQL010000733         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4198269, whole genome shotgun sequence.
ACCESSION   DATAQL010000733 DATAQL010000000
VERSION     DATAQL010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4198269"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..724)
                     /locus_tag="VHS97_12750"
     CDS             complement(113..724)
                     /locus_tag="VHS97_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449119.1"
                     /translation="MLPVQFGQSWFEIVAHQHDRQVGGTLYDANAQLTQGNAEFHRTL
                     NVDRLNADAAFLEVFLGNLRRQTQACPIGGHGANRRRRCREDIAAVDQPLEGFIDFVG
                     WKLLFQLANEVPKALPTFSYCGRDRAIERAVKKKLPVLGIEAHDIGRQHIDGEIRREP
                     RNVFAVTQRKGVLMIACHEVSTRTFIATPPVQGALFARQHSVV"
     gene            1343..>2436
                     /locus_tag="VHS97_12755"
     CDS             1343..>2436
                     /locus_tag="VHS97_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEX3449120.1"
                     /translation="MSGGSFGKGSNAEQGEASALMEAIERYSGIFQGNEIRVTRRFTD
                     FSSGDAIFPNDVLLFSDAQYRRNQLPTPVSDEPQPPAPFDRSAEIEWSPVWSLRDERF
                     KYLPTALLYFFYRVPGQINADSNGCAAGNTLEEAIVQGFLELVERDAYAIWWYNRLQR
                     PEVDLGKFDDSYVRDLQTQLAETGRRLWVLDVTSDLGVPTYVAITHWMQNGQENIEFG
                     SGAHFDPRIAALRALTELNQFLSIGLMGGGTGEKSSLDGTTPLRLQNHAFLTPSNMSA
                     AQPGLGSEFGHLNTREQVTACVALAKQAGLDFLVLDQTRPDIEVPVVRVVVPGMRHFY
                     RRFGPGRLYDVPIKLGLRDRPLAENELNPIL"
BASE COUNT          476 a          731 c          728 g          501 t
ORIGIN      
        1 catcgacgag gcgagctgcg ccgttttgtt gtgttgcccg cgtgttgcgc gtgggcggtg
       61 ataaaagcga tagattcgga agccgagaga tccggaaacg ctggcgcacg aactacacga
      121 cggagtgctg gcgagcaaac aatgcacctt gcactggcgg agttgcgatg aaggtgcgcg
      181 tactgacctc atgacaggcg atcataagaa cacctttacg ctgcgtaaca gcaaagacgt
      241 tccgcggttc gcgccgaatt tcaccgtcta tgtgctgccg cccgatgtcg tgtgcctcta
      301 ttccgaggac cggaagtttt ttcttcacgg cgcgctctat tgcgcggtcg cgtccgcaat
      361 aggaaaaggt gggaagagct ttcgggacct cgttcgcgag ctggaaaaga agtttccatc
      421 cgacaaagtc gatgaagcct tcaagcggct ggtcgaccgc cgctatatcc tcccgacatc
      481 gccttcttcg gttggcgccg tggccgccta ttgggcaagc ctgggtctgt cgccggagat
      541 tgccgagaaa aacctccaga aatgccgcgt ccgcgttcaa tcgatcgacg ttgagggtgc
      601 gatggaactc ggcgttgcct tgagtgagtt gggcgttcgc gtcgtaaagc gttcccccga
      661 cctgacgatc atgctggtga gcgactatct cgaaccacga ctggccgaat tgaaccggca
      721 gcatctgtcg gaccgtaccc cttggctgct cgtccaaccc tcaggcattt ttccgctggt
      781 ggggccggtg ttcagcccag gcgagagcgc ctgttggacc tgtcttttcg atcgcatgat
      841 acggaacagg gaagtcaagg gatttctcga ccgcagttcg gcgcgccgcg tcgccctttc
      901 gtctctcgcc cggaacacgt tgggacagag tgccatccag cttgcagcca tcgagatcgc
      961 gaaagcgatc gccaccggct tccgcacgga tttgggcaat cacatcatca gcctcgacct
     1021 gtcgggctcg accatggtga agcattacgt ggcccaccgc cctcaatgcc cgtcctgcgg
     1081 ccgcaagaag ctacgcgatc cgggccgcgc gccggtaccg atcgaactgg gccccggggc
     1141 caagctggtc atgacgagcg ggggctaccg gaccatttcg tcgcgggcca cggtcgcacg
     1201 ttttcggaag cacgtaagtc ccctgaccgg agtggtctcg cgtctcgaac gtatcgaagc
     1261 cgacttgccc ttgaactcca actaccgtgc aacacacaat ttttcggggc cggccggaac
     1321 ggtcaatgag ctcagagcgg gattgagcgg cggcagcttc ggcaagggca gcaatgccga
     1381 gcagggtgaa gccagcgcgc tcatggaggc gatcgagcgc tattccggaa tttttcaggg
     1441 caacgaaatc agagtaacac ggcggttcac ggatttctcg tcgggcgatg ccatttttcc
     1501 gaatgacgtc ctgctgttca gcgacgcgca gtaccggcga aatcagttgc caacgcccgt
     1561 ttcggacgaa ccgcagccgc cggccccatt cgatcgatct gccgagatcg aatggtcgcc
     1621 agtctggtcg ctgcgcgacg aacgcttcaa atatcttccc accgccctct tgtatttttt
     1681 ctaccgggtc cctggccaga tcaacgcgga ttccaacggc tgcgcggccg gcaacacgct
     1741 cgaggaagcg atcgtccagg gtttcctcga actggtggaa cgggatgcat acgccatctg
     1801 gtggtacaat cgcttgcagc ggccggaagt ggacctcggt aagttcgacg attcctacgt
     1861 gcgtgatttg caaacccagc tcgccgaaac cggacgccgg ctttgggtac tcgacgtcac
     1921 cagcgatctc ggcgttccga cctatgtggc gataacgcac tggatgcaaa atgggcagga
     1981 aaatatcgag tttggttctg gagcgcattt tgatccccga atcgccgcgc tgcgtgcgtt
     2041 gacggagttg aaccagtttc tgtccatcgg cctgatgggc ggcggcaccg gagaaaaatc
     2101 aagccttgac ggcaccacgc cactgcgcct ccagaaccat gcctttttga cgccaagcaa
     2161 catgtcggcc gcccaacctg gcctcgggtc ggagttcggc catctcaaca cacgcgagca
     2221 ggtgaccgct tgcgtggcgc tcgccaaaca agcgggcctt gattttctcg tcctcgatca
     2281 gacgcgcccc gacatcgagg tgccggtcgt ccgagtagtc gttccgggaa tgcggcattt
     2341 ctatcgtcgt ttcgggcctg gccggcttta cgatgttccg atcaagctcg gattgcgtga
     2401 ccggccgctg gcggaaaacg agctcaatcc gattct
//
LOCUS       DATAQL010000734         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4198354, whole genome shotgun sequence.
ACCESSION   DATAQL010000734 DATAQL010000000
VERSION     DATAQL010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4198354"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VHS97_12760"
     CDS             complement(<1..1120)
                     /locus_tag="VHS97_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX3449121.1"
                     /translation="MSDQPQPPDDSNGAGASAHASTRTIRVAAMARVEGEGALTVHLR
                     DGQVDDVQLRIFEAPRFFEALLRGRFIAEVPDITARICGICPVAYQMSACHALEALLG
                     VTLPDSLRALRRLLYCGEWIESHVLHIAFLHAPDFLGYESAFAMAQDRPEFADRLRDA
                     LQLKKAGNAIVDLVGGRAIHPINVRLGGFYRAPTKSQLASLAEQLKSVQKITRDLVRW
                     TASLDFPVMEHDYEFVALAHPSEYPMNEGRLVSNRGMDIAMHQFPEQFEEYQVPYSNA
                     LQCRRQGGGAYFVGPLARWNLCADHAGSEIQQLACETGISWPCRNPYVIIVARALETA
                     YAVDEAIRVIEEYEIPANQAVMYEVRAGSAAWITEAPRG"
     gene            complement(1095..1892)
                     /locus_tag="VHS97_12765"
     CDS             complement(1095..1892)
                     /locus_tag="VHS97_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449122.1"
                     /translation="MTTTRPRLGVFKFASCDGCQLSLLDCEDQLLAVADRVEIAYFLE
                     ATRRPLEGEFDVALVEGSVSTPDQENEIRDIRRRSRFLITLGACASHGGIQGLRNFGS
                     LNVDNALAIVYASPQYVETLACSRPASAYVAVDLELPGCPINKNQLIGVLGQMLVGAR
                     PKVPNHALCLECKRQHLVCILVSHGSPCMGPVTSTGCGVLCPSYDRSCFSCFGPSVQP
                     NTAALAQRFVQLGLKADEIQRLFQTFYANADEFKEEGLRHVRPTSTA"
     gene            complement(1929..2822)
                     /locus_tag="VHS97_12770"
     CDS             complement(1929..2822)
                     /locus_tag="VHS97_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX3449123.1"
                     /translation="MTPANPAITSPASMTRACTPPDADTLCPQPAVVLSVRSEAPRVA
                     TFSLAFGDQERQSCYQVKPGQFNMIYVPGVGEVPISVSHLPEEGSGIGHTIRFTGRVT
                     DVIGKLGPGAVVGLRGPYGRGWPVEQARGRDVLLVTGGLGLAPMRPVVRALIADRGQY
                     GRVMLLHGARQPADLLYVSEYPDWEGLGIEMMVTVDHADQAWRGRVGVVPVLFNRLQI
                     DPERTIVLTCGPEVLMRLAVTEALNRRISDADIYYSMERNMQCAVGMCGHCQLGPAFL
                     CKDGPVFAHRELAPFFRQELL"
BASE COUNT          596 a          917 c          918 g          553 t
ORIGIN      
        1 tgccacgcgg ggcttccgtg atccacgccg ccgagccggc gcgaacctca tacattacgg
       61 cttgattcgc tgggatttcg tattcctcga tcactcgaat agcctcgtcc accgcgtatg
      121 ccgtttcgag agcacgggcc acgatgatga cataaggatt ccggcagggc caggagatcc
      181 cggtctcgca tgcgagctgc tggatttcgg atccagcatg atcagcgcat aagttccatc
      241 gggccagcgg accaacgaaa tacgcgccac ctccctggcg acggcactgc agggcgttcg
      301 aatacggcac ctggtactcc tcaaactgtt cggggaactg gtgcatcgcg atgtccatgc
      361 ctcggttgga gacgagccgt ccctcgttca tcgggtactc cgacggatgc gcgagggcca
      421 cgaattcgta gtcgtgctcc attaccggaa agtccagact cgccgtccat cgaacgagat
      481 ctcgggtgat tttctggacg cttttcaact gctccgccag cgatgcgagc tgagacttcg
      541 tgggggcgcg gtagaagcca ccaagcctca cgttgatcgg atggatcgcg cggccaccca
      601 ccagatcgac tatggcattg ccggcttttt tcaattgcag cgcatcgcgc agcctgtccg
      661 cgaattccgg tcggtcctgt gccatggcaa aggcactttc gtaccccaga aagtcgggag
      721 cgtgtaggaa ggcgatatgc agcacatggc tctcaatcca ctcgccgcag tagagcaaac
      781 gtcgcagcgc ccgcaatgag tcgggcaggg taacgcccag aagcgcttcc agggcgtggc
      841 aggcactcat ctggtaggcc actgggcaga tgccgcagat ccgggccgtg atgtcgggta
      901 cttcggcgat gaaccggccc cgcaaaagcg cttcgaagaa ccgaggcgcc tcaaagatgc
      961 gcaattgtac gtcgtccacc tggccatcgc gcaggtgaac ggtcagcgct ccctccccct
     1021 caacgcgcgc catggccgca acgcgtatgg tccgcgtgct ggcatgagcc gaagcccccg
     1081 cgccgttgct atcatcaggc ggttgaggtt ggtctgacat gacgtaaccc ttcttccttg
     1141 aactcgtcag cgttggcgta gaacgtctgg aagagccgct gaatttcgtc ggctttgagt
     1201 ccgagttgca caaaacgttg ggcaagcgcc gctgtgttgg gctggacgct cgggccaaag
     1261 caactgaagc aagagcggtc gtaactgggg cagagcacgc cgcacccagt ggacgttacg
     1321 ggccccatac acggagaccc gtgcgacacc aagatgcaca ccaaatgctg ccgtttgcat
     1381 tcgaggcaga gagcgtggtt aggtaccttc ggtcgtgcgc caacgagcat ttgtcccagc
     1441 actccaatga gctgattctt gttaatgggg cagccgggaa gttcgaggtc gacagccaca
     1501 taggcgctgg ccggccgcga gcaggccagg gtttcgacgt attgcggcga cgcatacacg
     1561 atggccagtg cgttgtcgac gttcaggctg ccgaaattgc gcaggccctg gattccaccg
     1621 tgcgaggcac aggctcccag cgtaatcaag aatcggctcc ggcgacggat gtcgcgaatc
     1681 tcattttcct ggtcgggtgt ggagaccgag ccttcgacca gggccacgtc gaattcgccc
     1741 tcgagcggcc gacgagtcgc ctcaaggaaa taggcgattt cgactcggtc ggccaccgcc
     1801 aacaattggt cttcgcagtc gagcagtgag agctgacagc cgtcgcacga tgcaaactta
     1861 aagacgccca gtcgtggcct tgttgtggtc ataggcttag tggagtaaga ggtccggttc
     1921 tttccggatc agagtaactc ctgacggaag aacggcgcga gctcgcggtg agcgaagacg
     1981 gggccgtctt tgcagaggaa cgcgggcccg agttggcagt ggccacacat gcccaccgca
     2041 cactgcatgt ttcgctccat tgagtagtaa atatccgcat cgctgatccg acggttgaga
     2101 gcctccgtga cggccaaacg catcaggacc tcggggccgc aagtcagtac gatcgtgcgt
     2161 tcggggtcga tttggaggcg attaaatagc accgggacaa caccaacccg cccgcgccag
     2221 gcttggtcgg cgtgatcgac ggtgaccatc atttcgatcc ccaggccttc ccaatcggga
     2281 tactccgaaa cgtacaggag atcggccggc tggcgcgcgc cgtgcaaaag catgacgcgg
     2341 ccgtactggc cgcggtccgc gatcagtgcc cgcaccacag gccgcattgg ggcgaggcca
     2401 agcccgccag ttaccaacaa cacgtcgcga ccccgcgcct gttcgacggg ccagcctcga
     2461 ccgtagggcc cgcgcaggcc cacgaccgcg cccgggccca gcttgccaat cacgtcggtg
     2521 acccttccag tgaagcgaat cgtgtggcct atccccgacc cttcctccgg aagatgtgaa
     2581 accgagatcg gcacctcgcc tacccctggc acgtagatca tgttgaattg accgggcttg
     2641 acctgatagc agctctgacg ctcctggtct ccaaacgcga gggaaaacgt cgccacgcgc
     2701 ggtgcttcgg acctgaccga gagcacaacg gctggttgcg ggcagagcgt gtctgcgtct
     2761 gggggagtgc acgcccgagt cattgaagcc gggctggtga tcgctggatt cgcgggagtc
     2821 atcacgcgat cgtgcctccg cggatggcgg ctacttcttc agtgatgtcg atcgccactg
     2881 ggcaccaggt gatgcagcgc ccgcagccga cgcatccgga cactccatat tgatcgatcc
     2941 aggaagccag cttgtgtgta agccactggc ggtagcgcga ccgg
//
LOCUS       DATAQL010000735         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4348504, whole genome shotgun sequence.
ACCESSION   DATAQL010000735 DATAQL010000000
VERSION     DATAQL010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4348504"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..612)
                     /locus_tag="VHS97_12775"
     CDS             complement(262..612)
                     /locus_tag="VHS97_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449124.1"
                     /translation="MPDVAALAGPPYYDLIFLGQPSPSGGTSASTPLWAALLARVQSA
                     LPSKKSPQFLTPLLYRGVAGSPPLGAAVCRDIAVGQNASSPEPGRGYHAGPGYDAVSG
                     WGTPIGTSLLTALS"
     gene            complement(745..1884)
                     /locus_tag="VHS97_12780"
     CDS             complement(745..1884)
                     /locus_tag="VHS97_12780"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEX3449125.1"
                     /translation="MAQSYVDLPGSERPQSATAKRIGDADQQAQVEVTIGLRGPALPG
                     ADGLPVKPLSRSEFEAKYASAKADAEKVAQVLTPFGLHVEDISLAGRSMRVSGTVSAM
                     EEAFRLKLGLYESATQSRYRGREGLIQVPAELKGIVTGVFGLDERRVARRKPKIGARA
                     AGAPAALGPSDLETRYAFPGGDAAGQRIAIAEFDGGYFPDDVSAYCNKFGRSSPQVTI
                     IPVGKKALTLAQIKALPKSQQVAELDSSGEVMMDVQIVAGLCPGAKIDVYFAPFTQKG
                     WVDLLNKVAANGAGIPVALSVSWGMPEDDPSWSAAARAAINDRLQAAALLGITVCIAS
                     GDDGSGDEISDGRAHVDFPSSSPFALSVGGTMLAGTSTQATEEVW"
     gene            complement(1942..2214)
                     /locus_tag="VHS97_12785"
     CDS             complement(1942..2214)
                     /locus_tag="VHS97_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449126.1"
                     /translation="MSGKRYADKFKIEAVKQVTEHGRFLVAVAQRLETGRRSFTSRVR
                     AALRAKRLLSVYKPLAGRWGRRRNTRTAYDGRAVVPNFTKCMVPAS"
     gene            complement(2302..>2731)
                     /locus_tag="VHS97_12790"
     CDS             complement(2302..>2731)
                     /locus_tag="VHS97_12790"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3449127.1"
                     /translation="CGLSRPSLLAVASPVASATGTSEREPIRTVALKFSAFNRHDAAT
                     IEALYASDAVLRSPDYPELLGNSKIADTYRWIFDAIPDAKDSIVTIESTANHVYVQFV
                     LSGHLKGAENKPINVRIMSVYTVKDHLVVSDSTYYDRKTQ"
BASE COUNT          543 a          830 c          824 g          534 t
ORIGIN      
        1 aggtgatcga acgagcggtt ctcgagcatg agcacgaaga cgtgttttac gggcatgcaa
       61 ggctcctatt aataggatct gtcggaatgg actaccttga gaaacaagac gctcgaaagc
      121 ttcagtgtga ggcctgctca aaggcctcgg gactgacacc atcgcgccac ccggaaagtt
      181 gccgggcagc gccaccatgt aatcgctcct gcttgttgag caggagcgat taccagcaag
      241 aacacagggt caacccatcg actaagagag tgccgtcaac aagctggtgc caataggtgt
      301 gccccacccg gatacagcgt catagcccgg gccggcgtga tatccccggc cgggctcggg
      361 gctggacgcg ttctggccga cggcaatgtc gcggcatacc gcggcaccaa ggggtggact
      421 acccgcgacg ccgcgatata gcagtggcgt cagaaattgt ggcgactttt tgctcggcaa
      481 ggcactctgg acgcgcgcaa gcagggccgc ccatagcggt gtggacgcac tcgtaccacc
      541 actggggctg ggctggccga gaaaaatcag gtcgtagtac ggcggacccg ccagtgccgc
      601 cacgtcgggc atgacgcgcc ccgggatact gccgctgttg agagaggtga cactgacgtt
      661 ctgccaggac gggcgctgga agtagatgct gacgccgcca ccggtcgcac cgcctccgtt
      721 actggtgcgc cgacccggcg actctcacca cacttcctcg gtcgcctgcg tgctggtgcc
      781 cgctagcatc gtgccaccca cgctcagtgc gaacgggctt gagctcggaa aatcaacatg
      841 ggcgcggccg tcggaaatct catcacccga gccatcgtcg cccgaggcta tgcaaaccgt
      901 tatgccgagc agcgcagcgg cctgcaggcg atcattgatg gccgcgcgcg ccgccgcact
      961 ccaacttgga tcatcctccg gcatgcccca gcttacggat agcgccaccg ggatgcctgc
     1021 gccattggca gccactttgt tcagcaggtc gacccaccct ttctgagtga agggcgcaaa
     1081 atagacatct atctttgcac cagggcacag acctgcgacg atttgcacgt ccatcatcac
     1141 ctcgccagaa gagtccagct ccgccacctg ctgactcttc ggcagcgcct tgatctgtgc
     1201 cagcgtgagt gcctttttgc cgactggaat gatcgtcact tgcggcgatg aacgaccgaa
     1261 cttgttgcaa taggcggaga cgtcgtcggg aaagtagccg ccgtcgaatt cggcaatggc
     1321 aatgcgttgg ccggcggcat ctccaccggg gaaggcgtag cgggtttcga ggtcggacgg
     1381 ccctagcgcc gcaggcgcac cggcggcacg cgcgccgatc ttgggtttgc gccgtgccac
     1441 gcgacgctca tccaggccga acacgccggt gacgatgccc ttgagttcgg ccggcacctg
     1501 gatcaaaccc tcgcgtccgc gatatcgact ctgcgtcgcg ctttcgtaca ggccaagctt
     1561 gagccgaaag gcctcctcca tcgcggagac ggtgccactg acgcgcatgc tacggccagc
     1621 gagcgatatg tcttcgacat gcaggccgaa gggggtaagc acttgcgcca ccttctccgc
     1681 gtcagccttg gcggaggcgt acttcgcctc gaattcgctc cggctcaacg gcttgacggg
     1741 taacccgtcg gcacctggca gggccggccc acgcaggcca atggtcactt cgacctgtgc
     1801 ctgctgatcg gcatcgccaa tgcgcttggc agtggcactc tgcgggcgtt cgctaccggg
     1861 aaggtcgaca taagactgag ccatggctgg atctcctcat caagttgcgt tgtcgcacgg
     1921 caagcagcaa gccgcgcgat gctaactcgc gggaaccata cactttgtga agttgggtac
     1981 aactgcacgg ccgtcgtatg ccgttcgggt gttacgtcga cggccccatc gaccggccag
     2041 aggtttgtag acactcagga gccgctttgc gcgtagcgcc gctcgaactc tactggtgaa
     2101 agatcggcgg ccggtctcca gccgctgggc gacggccacc aggaagcgcc catgctcggt
     2161 cacttgcttg actgcttcga tcttgaactt atccgcgtac cgcttgccac tcataaagac
     2221 ctcctcggtt gccagaaatc atggctccga gttgtctacg aaaggctgac cggtccaggt
     2281 tagtggcgca aactgccatt gctactgggt ctttcgatcg tagtaggtag agtcactcac
     2341 aaccaaatga tccttgaccg tgtaaaccga catgattcga acgttgatgg gtttgttttc
     2401 cgcacctttc agatggccgg acaggacgaa ctgaacgtag acatggttcg ccgtactttc
     2461 gatggtgacg atgctgtctt tggcgtcagg tatggcgtcg aaaatccaac ggtatgtatc
     2521 ggcgatcttc gagttgccaa gaagctcagg atagtctggg ctacgcagga cggcatcgct
     2581 ggcgtagagg gcttcgatcg tagcggcgtc gtgtcgattg aatgccgaaa atttcaacgc
     2641 aaccgtcctt attggctcac gttctgatgt gccggtggct gaagcaaccg gacttgcgac
     2701 tgctagtaaa gacggcctcg ataggccaca t
//
LOCUS       DATAQL010000736         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4807844, whole genome shotgun sequence.
ACCESSION   DATAQL010000736 DATAQL010000000
VERSION     DATAQL010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4807844"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VHS97_12795"
     CDS             <1..1266
                     /locus_tag="VHS97_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX3449128.1"
                     /translation="LEFDQVLVGEVWICSGQSNMQWSVAAAKDADLEIATAKYPLIRL
                     ISVPNVGTQEPQKDFRGRWQPCQPDTVGRFSAVGYFFGRRLHQVLDVPVGLINDAWGG
                     SACEAWIRRDILAADEKYGPLLKRWDRLEKNLPQAKAEYESRLAEWTKAAAKARADGK
                     EAPRRPVNPEDQMRGNSRPGNIYNGVLKPTIGYGIRGAIWYQGESNAGRAYQYRDMFP
                     LMIKSWRDEWGQGDFSFYWVQLADYQAEVPEPRDSMWAELREAQTMTMKLPKTGEAVI
                     IDVGDAHDIHPTNKQDVANRLARWALARDYGVKIAAQSPTYAAMEKHGNKIVLKFKEV
                     GRGLKTADVAEARGFAIAGDDHKFVWAKAKIVGPASIEVWSDNVSEPAAVRYAWADNP
                     VCNIYSAEGLPLTPFRTDDFPALTVNKLE"
     gene            1328..>3333
                     /locus_tag="VHS97_12800"
     CDS             1328..>3333
                     /locus_tag="VHS97_12800"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEX3449129.1"
                     /translation="MRRPMMLRHLLRSVCAVWFVLTASAACAREDTASHPAATSPDRQ
                     IRIEFFLEERDGARAAPAYSVSFRGRPVVLPSRLGIDLAGRQGLGRDSTIEGVRTRTI
                     NETYSQHPGKRSRVVDHCEEVVVALRERATPTWRWEVVLRAYNDGVALRYRFPAQESL
                     QRLAIAGERTQIQLPDNAQSYTLPLNSYTTSHEVRYQKKPVSEIPAEWLLGLPLLTEL
                     PGTGWLAVLEANLTDYAGLYLARGADGGAVLDCRLSPRPGEPKVAVRADLPHDSPWRV
                     FMIADKVERLVESDLVLNLNAPCALTDTSWIRTGKTTFPWWNGFHEDKVPFEMGLNTD
                     TAKYYIDFCAEAGIPYHSLDGKDNIAWYGGTIIPHGGGDITRGIQGLDLGEVLRYAAS
                     KGVKIRLWMHWRGAEAHMDRAFPLYHQWGVEGVMLDFMDRDDQEMVNFLHRALEKAAA
                     NQLTVTLHGVAAPTGLERTFPNLLTNEGVLNLEYDKWDKQGVTPEHELTVVFTRMLAG
                     PLDFHQGSLRGVPVEKFQPRNVAPLVIGTPCRMLASYVVLQNHLPMVADYPSAYRGHP
                     ALPVLAAIPTTWDDTRCLAGKVGELIVMARQYGAEWWLGAMGGREPREVEIPLGFLGP
                     GRFRAEVYRDDLSAAARLARRTEDVAAANSVRAPLAPAGGLLIRL"
BASE COUNT          641 a         1049 c         1051 g          592 t
ORIGIN      
        1 ctcgaattcg atcaagtgct cgtgggtgag gtctggatct gctcaggcca gtccaatatg
       61 cagtggtcgg tggccgcggc caaggacgcg gatcttgaaa tcgcaaccgc caagtatccc
      121 ttgatccgac tgatcagcgt ccccaacgtc ggcactcagg agccccagaa agacttccgc
      181 ggccggtggc agccatgcca accggatacg gtcggccggt tctctgcagt gggctacttt
      241 ttcgggcgac gactgcacca ggtgctcgac gttcccgttg gcttgatcaa cgacgcatgg
      301 ggtggatcgg cgtgcgaggc ctggattcgc cgcgacatct tggccgcgga tgagaaatat
      361 ggccccctgc tcaagcgctg ggaccggctc gagaaaaacc tgccccaagc caaggccgag
      421 tacgaatcaa gactggccga atggacgaaa gccgccgcca aagccagggc cgatggcaag
      481 gaggcgcccc gtcgtccagt gaacccagag gatcagatgc gcggcaactc tcggccgggg
      541 aacatctaca acggagtgct caaaccgacg attggctacg gcatccgcgg cgctatttgg
      601 tatcaaggcg aatccaatgc cggccgggcg tatcagtatc gcgacatgtt tccgcttatg
      661 atcaagagct ggcgcgacga gtggggacag ggagacttct cgttttactg ggtgcagctc
      721 gccgattacc aagccgaagt acccgagccg agagacagca tgtgggcgga gctgcgcgag
      781 gcccagacaa tgaccatgaa gctccccaag acgggcgaag cggtgattat tgatgtcggc
      841 gacgcgcatg atatccatcc caccaacaag caggatgttg ccaatcgctt ggcacgatgg
      901 gccctggcgc gcgactacgg cgtgaagatc gccgcacaga gcccgaccta cgcggcgatg
      961 gagaaacacg gcaacaagat cgttctgaag ttcaaggaag tgggacgtgg tctcaagacc
     1021 gccgatgtcg ccgaggcgcg gggctttgcc atcgccggcg atgaccacaa gttcgtctgg
     1081 gccaaggcga aaatcgtcgg cccggcctcg atcgaagtct ggtccgacaa tgtgtcagag
     1141 ccggcagccg ttcgctatgc ctgggccgat aacccggtct gcaacatcta tagtgccgag
     1201 ggccttccac tcacgccgtt ccgcaccgac gacttcccgg cactgacggt gaacaaactc
     1261 gagtagaagg gctcttttca accaatgcgc cacgggccgc aatcaatctc aaacgacgag
     1321 taggatgatg cggagaccga tgatgctgcg ccacctgttg cgatcggttt gcgcggtctg
     1381 gtttgtctta accgcatctg ctgcgtgcgc gcgtgaggac accgcatcgc atcccgccgc
     1441 aacctcgccc gaccgtcaga ttcgaataga gttctttctc gaagagcggg acggggcgcg
     1501 ggccgcacct gcctattccg tctcctttcg agggcggccc gtcgtcctcc cgtcccggct
     1561 aggtattgac ctggccggca ggcaaggcct tggccgcgac tcgacgatcg agggagttcg
     1621 gacacggacg atcaacgaaa cctatagcca gcatccggga aagcgcagcc gggtggtcga
     1681 ccattgcgaa gaggtcgttg tcgcgcttcg cgagcgggcc acaccgacgt ggcgctggga
     1741 ggtcgtcctt cgcgcttaca acgacggggt cgccttacgt taccgctttc ctgcccagga
     1801 gagcttgcaa cgcctcgcga tcgccggcga acgaacccag atccaattgc ccgacaacgc
     1861 ccagtcatat acactccccc tgaatagcta caccacctcc cacgaggtcc gctaccaaaa
     1921 gaagccagtg tccgagattc ctgcggagtg gcttctcggc ttgccgctct tgaccgagtt
     1981 gccggggacc ggctggctcg cggtgttgga ggccaacctc accgactacg cggggctgta
     2041 cctggcgcgc ggtgccgacg gaggggccgt cctcgactgt cggctatccc cacggcccgg
     2101 cgagccgaaa gtcgcggtcc gcgcggatct gccgcacgac tcgccctggc gcgtcttcat
     2161 gatcgccgac aaggtggagc gacttgtcga gtctgacctc gtgctcaacc tgaacgcgcc
     2221 ctgcgccctg accgatactt cctggatccg gaccggcaag acgacattcc cttggtggaa
     2281 cggcttccac gaagacaaag ttcccttcga gatggggctg aacacggaca cggcgaagta
     2341 ctacatcgac ttctgtgcgg aggcgggcat tccgtaccac tcgctcgatg gcaaagataa
     2401 tattgcatgg tacggcggca cgatcatccc gcatggaggg ggcgacatca cccgtggtat
     2461 ccagggtctc gatctcggcg aagtgcttcg ttacgccgcg tcgaagggcg tcaagatccg
     2521 cctctggatg cactggcggg gggccgaggc ccacatggac cgggccttcc cgctctacca
     2581 tcagtggggc gtcgaagggg tgatgctcga cttcatggac cgcgacgatc aagagatggt
     2641 caacttcctg catcgcgcgc tcgagaaggc cgccgcgaac cagttgacgg tcacgctgca
     2701 cggggttgcc gctccaacgg ggctggagcg gacttttccc aacttattga cgaacgaggg
     2761 tgtgttgaac ctggaatatg ataaatggga caagcagggc gtcacacccg agcacgagct
     2821 gaccgtggtg tttacccgta tgctggctgg cccgctcgac ttccaccaag gttcgctccg
     2881 tggcgtaccc gtggagaaat tccagccgcg caacgtggct ccgctggtca tagggacacc
     2941 ctgccggatg ctcgcgtcct atgtcgttct ccagaaccac ctgccgatgg tcgccgacta
     3001 cccgtccgcc tatcgcggcc acccggcgct accggtcctg gcggcgatcc caacgacctg
     3061 ggacgacacc cgctgcctgg ccggcaaggt cggggagttg atcgtcatgg cccggcagta
     3121 cggggccgag tggtggttag gcgcgatggg cggccgagaa cctcgcgagg ttgaaatccc
     3181 gctgggcttc ctcggtcccg gccggttccg ggcggaggtt tatcgggacg acctgagtgc
     3241 cgcggcccgc ctcgcccgac gcacggagga cgttgccgcc gcgaattccg tgcgcgcgcc
     3301 gctcgcgccg gcgggtggcc tgctgatccg act
//
LOCUS       DATAQL010000737         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3433045, whole genome shotgun sequence.
ACCESSION   DATAQL010000737 DATAQL010000000
VERSION     DATAQL010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3433045"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="VHS97_12805"
     CDS             complement(<1..1899)
                     /locus_tag="VHS97_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX3449130.1"
                     /translation="MGRWSMVEPVEVAAEPTHLESLVRNLKDLRKSPDSGNVAGPPDS
                     FGLAPPVATVKLWGAAAAASQSAAEPIAALALGKTVRNVQYVRDASTEAIEVADPKLL
                     SAVGLPLNDWRERVVMAVPSFQVASVTIRRPEQVIHAERGKRGQWRLSAPINVPANPA
                     KVESLIAALSSLRVVDGDKGFVADNVKDFAPFGLVSPLTIEMTTTRPGDSPLVLHVGK
                     PVPDQPERIYVRQGDQDDVVIVDAKALSEVPTGAAALRSQRVADIEPAAVTEIQIETK
                     IGTFAVSKRPNQWELTSPRTEKADTESVMALLNRIDALQASEFFEPSRIGNAELDPPV
                     MTIKIWESARARSGTSASVGEPTLVLRIGKHDALRKTVFARLENDQVILALPDTILEV
                     LPRNAFAFRDLEVLSLNPADIRKLTITRGVRIDELEPDKNGEPNRWRLLRPTNARADA
                     RSVTQVIAVLSRLRADQLVSDSADDLKKFGLDHPVLEIAWESDRAHRLVVGSPVPRSS
                     AYYAKTDDLSFIFTIKTEVLKPFEAEFRDHTVLSFPAAKAQRVVLRWTWPKRDVAFRQ
                     RVQAAKGQPEWVDEPGSDAAGIDQSRIKPLVKALSQLETPRFVQYDGEIPPVTGLLRP
                     RLTVEVELS"
BASE COUNT          378 a          603 c          582 g          390 t
ORIGIN      
        1 acttagctcg acctcgaccg tcagccgtgg acggagcaaa cccgtcacgg gcggaatctc
       61 gccgtcatac tgcacaaaac ggggcgtttc gagctgcgag agtgctttca cgagtggctt
      121 aatccgcgat tggtcgatgc cagctgcgtc agagccgggc tcatcgaccc attccggctg
      181 gcctttggcc gcttgcacgc gctgacggaa ggcgacatca cgtttgggcc aagtccagcg
      241 caacacgact cgctgggcct tggccgccgg gaatgagagc acagtatgat cgcgaaactc
      301 ggcttcaaat ggtttcaaga cttcggtctt gattgtgaaa atgaacgata aatcgtcggt
      361 tttggcgtaa taggccgacg atcgcgggac tggcgagccg accacaaggc ggtgcgctcg
      421 atccgattcc caggcgattt cgaggaccgg atgatcaagg ccgaactttt tcaggtcgtc
      481 tgcggaatca gagacaagct gatcggcgcg cagcctggac aagaccgcga tcacctgggt
      541 gaccgatcgc gcatcggcgc gggcattagt cggccgaagt aaacgccacc gattgggttc
      601 gccgttcttg tccggctcga gctcgtcgat cctgacgccg cgagtgatcg tgagcttgcg
      661 aatgtcggct ggattcaacg acaacacctc gagatcgcgg aaggcaaacg cgttcctggg
      721 caagacttca agaatcgtgt cgggcaaagc gaggatgacc tggtcgttct ccaaccgggc
      781 gaagaccgtt ttccgcagcg cgtcatgctt gccaatccgc aagacgagtg ttggctcacc
      841 cacagacgcc gacgtccccg atcgtgccct tgcagattcc cagatcttga tcgtcattac
      901 ggggggatcg agctccgcat tgccgatccg gctcggctcg aagaactcgc ttgcctggag
      961 cgcgtcgatt cgattgagca aagccattac cgattccgtg tcggctttct cggtgcgcgg
     1021 cgaagtgagc tcccattgat tgggtcgttt actgaccgca aacgtaccga tcttggtctc
     1081 gatctggatc tcggtcaccg cggccggctc gatgtcggcg acccgctggc tccgcaaggc
     1141 ggccgccccc gtgggaacct cagacaatgc tttggcgtcg acaatgacga cgtcgtcctg
     1201 gtcaccctga cggacgtaga tccgctccgg ttggtccggc acgggcttgc cgacgtggag
     1261 caccagcggc gaatctcccg ggcgggttgt cgtcatctcg atggtcaacg gtgagaccag
     1321 accgaaggga gcaaagtctt tgacattatc cgcaacgaat cccttgtcgc catcaacgac
     1381 ccgcagggac gatagggcgg cgatcaagct ttcgaccttg gcaggattcg ccggcacatt
     1441 tatcggtgcc gacagccgcc attgccccct cttaccgcgt tcggcgtgga tgacctgttc
     1501 gggtcgtctg atcgtcaccg acgcgacctg aaaactggga acagccatca cgacgcgctc
     1561 gcgccaatcg tttaagggca acccgaccgc gcttaacaac ttcggatcgg cgacttcaat
     1621 tgcctcggtg ctcgcgtcac ggacgtactg gacattgcgc accgttttgc caagagccaa
     1681 ggctgcgatc ggctccgcgg cagattgact cgccgcggcc gctgcccccc aaagcttgac
     1741 agttgccacc gggggagcca ggccaaacga atcaggcggg ccggcgacgt tgcctgagtc
     1801 tggcgactta cgcaggtctt tgaggtttcg aaccaagctt tccaagtgcg tgggctcggc
     1861 tgctacttcg accggctcga ccatgctcca tcgacccatt ccctgcccgc gccgctcgaa
     1921 gaccagacgc tcttttcccc gatcgatctc gag
//
LOCUS       DATAQL010000738         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1907819, whole genome shotgun sequence.
ACCESSION   DATAQL010000738 DATAQL010000000
VERSION     DATAQL010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907819"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2152
                     /locus_tag="VHS97_12810"
     CDS             <1..2152
                     /locus_tag="VHS97_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX3449131.1"
                     /translation="GKPEESSLIEAIRYEGEVRMPPNKKLKDDEIAALSDWVKRGAFW
                     PEPRLGRTDRAGVKSPATSTDAAPPAGVSAQDRSFWSFQPVTNPPPPAVKESAWPQSS
                     VDRFILAKLEENNLAPAPPADRSTLIRRAYFDLIGLPPTAEEVQAFVHDERPDAFGKV
                     VDDLLASPHYGERWGRYWLDVARYGEDQAHSFQPRLYPYGFRYRDWVVRALNRDMPYD
                     QFVIEQIAGDLIDGPDRAERLSALGFFACGPVYYGDSKKHDQYADRIDTLTRGFLGLT
                     VACARCHDHKYDPIPTTDYYALAGVFASTEYVEVPSAPKEQIEAYDKAQALVQAKDKE
                     ITEVVKAEAERLKQKVAGNNQLKQFERMLMGEPKAKVKALRAELDLLKKNAPPKYPVI
                     HTLGEASNAMDMPVLIRGNADTPGAKVSRRFLAILGGDRSTFQHGSGRLELAHAIASP
                     ENPLTARTMVNRIWQHHFGRGLVASSSNFGALGDRPSHPELLDWLAHQFISSGWSIKE
                     MHRTIMLSAAYQQSSRFESGAFDKDPGNALLWRMNRRRLDVEAWRDSMLAVAGRLDRT
                     IGGRSISLDAPTNQRRTLYAAISRHDLAWMLRLFDFPDPNITSGGRALTTVPLQQLFV
                     LNSDFMMTSARAVAAQLQAVSGPGQDDGERIRQAYLFLYGREARPHEVELAVAYLQTP
                     EPGAAGESTAHDSPNRWERYAQALLASNEFLFVD"
     gene            2328..3806
                     /locus_tag="VHS97_12815"
     CDS             2328..3806
                     /locus_tag="VHS97_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3449132.1"
                     /translation="MRQRNTQPLSGLGPAVGSQPSLTRRALFSRIGGGFGTLGLAGVL
                     ADAGMLVPAMASPPGAVQNSEGAVLNHPLAPRPPHFPARAKRVIFLFMNGGPSHVDTF
                     DPKPALVRYQGQSPSAKLVTGRTKVGKVMPSPFSARAHGQSGIEVTELFPHVASCIDE
                     ICVLRSMYTDNPNHEPSLLMMNSGNMQPIRPSLGSWLTFGLGTENQNLPGFVVLCPGK
                     PVVGPQLWSNSFLPGIYQGTHIQNQTIDPDRIIRDVTNRRLSPLEQRSQLDLVQALNR
                     EHLAERSHDDALEARIDSLEMAFRMQFEAREAFDLGRESGLTRELYGNGEFASACLIA
                     RRLVERGVRVVQVYYGKDQPWDDHADINKHRDHAQKSDQPIAALLKDLKARGLLEDTL
                     VIWGGEFGRTPYSEGAKGRDHHSLGFSMWLAGGGVKGGYVHGATDELGMQAVENRMHV
                     HDLHATILHLMGLDHEHLTYRYSGRDFRLTDVSGRVAREILA"
BASE COUNT          933 a         1448 c         1374 g          946 t
ORIGIN      
        1 gggcaagcct gaagaaagct cgttgatcga ggcgatccgc tatgaagggg aggtgcggat
       61 gcctcccaat aaaaaactca aggatgacga gattgccgcc ctctccgact gggtcaaacg
      121 aggtgccttc tggcccgagc ctcgcctcgg ccgtacggat cgagccggtg tgaagtcgcc
      181 tgcgacctca accgacgccg caccgcccgc cggcgtatcc gcgcaagacc gttcgttctg
      241 gtctttccag ccggtcacaa acccgccgcc gccggcggta aaagaaagtg cgtggccaca
      301 gtcgtcggtc gataggttta ttctcgccaa gctcgaggaa aacaacctgg cgccggcacc
      361 gccggctgac agatcgaccc tgatccgccg cgcgtatttc gatctgatcg gtctaccgcc
      421 tactgccgag gaagtccaag cgttcgtcca tgacgagcgg ccggatgcgt ttggcaaggt
      481 ggtcgacgac ctcctggcct cgcctcacta cggcgagcgg tggggacgct actggctgga
      541 cgtcgcccgc tacggtgaag accaggctca ctcgttccaa cccaggctct atccctacgg
      601 gttccgctat cgtgactggg tggtccgagc ccttaaccgc gacatgccct acgatcaatt
      661 cgtgatcgag cagatcgctg gcgacttgat tgacgggccc gatcgggcgg agcggctatc
      721 ggcacttggc ttcttcgcct gtggcccggt ttattacgga gactccaaaa agcacgatca
      781 gtatgccgac cgtatcgaca ccctgacccg cggttttctt ggcctcaccg tcgcatgcgc
      841 ccgctgccac gatcacaagt acgacccgat cccgaccacc gattactacg ctcttgccgg
      901 cgttttcgcc agtaccgaat atgtggaagt tccgtccgca cccaaggaac agatcgaggc
      961 gtacgacaaa gcccaagccc tcgtccaggc caaggacaag gagatcacgg aggtcgtaaa
     1021 ggcggaagcg gagcgtttga aacagaaggt cgcgggcaac aaccagctca agcaattcga
     1081 gcgcatgctg atgggcgagc ccaaggcgaa agtaaaagcg ctccgcgccg agctcgacct
     1141 gttgaagaaa aacgcgccgc ccaaatatcc ggtgatccac accctgggcg aagcatccaa
     1201 tgcgatggac atgccggtct tgatacgcgg caacgcggat acacccgggg ccaaggtttc
     1261 gcggcgattt ctggcgatcc tggggggtga taggtctacg ttccagcacg gcagcggccg
     1321 cctcgagctt gcccatgcga ttgcgagtcc ggagaatccg ctgaccgcac gcacgatggt
     1381 caatcggatc tggcaacacc actttggccg cgggctggtc gcctcttcca gtaactttgg
     1441 tgccctcggc gaccgaccga gccaccccga attgcttgac tggctggctc atcagttcat
     1501 ttcgtccgga tggtcgatca aggagatgca ccggacgatc atgctctcgg cggcctacca
     1561 acagagtagc cgctttgaat caggggcctt cgacaaagac cctggcaacg ccttgctgtg
     1621 gaggatgaat cgccgacggt tggatgtcga agcctggcgc gattcgatgc tggccgtcgc
     1681 cggtcggctc gatcggacta ttggcggccg atcgatcagc ctcgatgcgc ccacgaatca
     1741 gcgccggacc ctctacgccg cgatcagccg gcacgacctg gcgtggatgc tccgcctttt
     1801 tgactttccc gatcccaaca tcaccagcgg cggtcgcgcc ctaaccaccg tccctttgca
     1861 acaacttttt gtgctcaata gtgacttcat gatgacaagc gcccgagccg tcgctgcgca
     1921 gttgcaagcg gtctcgggcc ccggccagga cgacggcgag cggatccgtc aagcctacct
     1981 gtttctctac ggccgggagg cgcggccgca cgaggtcgaa cttgcggttg cctatctcca
     2041 aacgccggaa cccggcgctg ccggagagtc gaccgctcac gacagtccaa accgctggga
     2101 acgatacgct caggccctcc ttgcatccaa tgaatttttg ttcgttgact gatattacga
     2161 attgttggac gtgcgccacc aggttttagg ttatccaggg cgttgccctg ggctgatctg
     2221 ttaatgccct tttgggcaac gatgcaaagg taccacttgg gatcacgccc cgtaggggca
     2281 atcataaact agcccagggc gacgccctgg gtacgaggtc cgtaaccatg cgacagagga
     2341 atactcagcc attaagcggg cttggtccgg ctgtcggttc acaaccgtcg ttgactcgcc
     2401 gcgctctctt ttcccggatt ggcggcggct ttggcacgct gggtttagcc ggcgtcctgg
     2461 ccgacgcagg tatgcttgtc cccgccatgg cttctccacc tggggcggtg caaaactcgg
     2521 agggggcggt tctcaaccat cctttggccc cccggcctcc ccactttccc gcaagggcca
     2581 agcgagtcat ctttctgttc atgaacggcg gcccgtcgca cgtcgacaca ttcgacccca
     2641 agccggccct ggtgcggtac caggggcagt ctccatccgc caaactcgtg acaggacgga
     2701 ccaaggtcgg caaagttatg ccctcgccgt tttctgcgcg ggcgcacgga cagagcggca
     2761 tcgaggtgac cgagcttttc ccgcacgttg ccagttgcat cgacgagatc tgcgtactcc
     2821 gctcgatgta caccgacaac cccaatcacg agccgtcgct cttgatgatg aactcgggca
     2881 acatgcagcc gatccggcca agcctgggtt catggctgac cttcggcctc ggtaccgaaa
     2941 accagaatct gcctggcttt gtcgtcttat gtcccggcaa gccggtcgtc ggtccgcaat
     3001 tatggagcaa tagctttctg cccggcattt accagggcac acacatccaa aatcagacga
     3061 tcgatccgga caggatcatt cgcgacgtta ccaaccgccg cctctcgccg ctcgagcagc
     3121 gctcccagct tgacctggtg caggcactga atcgcgagca cctcgccgag cgcagccacg
     3181 atgacgctct cgaagcgagg atcgattcgc ttgagatggc attccgcatg caattcgagg
     3241 cgcgcgaagc ctttgacctg gggcgtgagt cagggctgac gcgagaactt tacggcaacg
     3301 gcgagtttgc gagcgcttgc ttgatcgccc ggcgactcgt tgaacgaggc gtgcgcgttg
     3361 tccaggtcta ttacggcaaa gaccagccct gggacgatca tgcggatatc aacaagcacc
     3421 gcgaccatgc gcaaaagagc gatcagccca tcgccgccct gctcaaggat ttgaaagcgc
     3481 gcggactgct ggaagatacc ctggtgatct ggggaggtga atttggtagg acgccttatt
     3541 ccgaaggagc gaaggggcgt gatcaccaca gcctcggctt ttcaatgtgg ctggccgggg
     3601 gaggcgtgaa gggcggctac gtgcacggcg cgacggacga gctcgggatg caggcggtgg
     3661 aaaaccgcat gcacgtccat gatctgcacg ccacgatcct gcacctcatg gggctcgatc
     3721 atgaacacct gacctatcga tacagcggac gagacttccg cttgaccgac gtcagcggcc
     3781 gcgtcgcgcg cgagatcctt gcgtgaacgg cgctcgtcga ccgagacgtt cacttcattc
     3841 aatcaaggag ccggcactgg ctgtgttcac aaccggtgcc ggccaattcg acttaacccg
     3901 cctatgactt ctgtccccag aggacggtac acagccgcac gctgccggcg gtttcgctct
     3961 cgaacaaacc ctcattgaac cagcgtgtcc agccccgagg gcggcgagtc tcacgacgta
     4021 ctccaaaaac cttccgacgc ttcttggcgc tcataattgc ctccgaaggt aggagagggg
     4081 atatgatttg gtccacatca agtctactct tccccttcgc aagtacgata ccagcgcgac
     4141 agatcgcgcc ggaatgttgc attttcacca tatcaatacc ctctgctatc caaggtatcg
     4201 ggagcgcgct atctctcctc tgtgccataa caccttgtat ttgtggattt tgaatcatcg
     4261 atttcgttcc ggtgcttttc ggcgagctcc caatgcatgc gagccggggt tgatccggaa
     4321 cttacgcgcg gtttgtaagc attgcagcgt cgagcctgag cagccgtctt gtgtgatcga
     4381 cggtgcgatg ccatgaaccg gtccgagatc gcctccttat gttcgtgcgg agaagccggt
     4441 aaactgagac ctcgatcggg cgttccagcc tttcgatgac gatctgatgt ggaagcgcgt
     4501 aacggcacga cgcaatctta tcgtgtgata gagagctacg acgatgtcag gctggttaaa
     4561 cgatgcctgg gcgtatctgc gatcgttggc cgctggggtc gatttaccga gcgtcctgtc
     4621 atttctagga cggaagctgc ttgagggtct cttccaggcg gcggtggtcg ttgtaatcgg
     4681 gtggctggtt ttgtatcgcc a
//
LOCUS       DATAQL010000739         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_228120, whole genome shotgun sequence.
ACCESSION   DATAQL010000739 DATAQL010000000
VERSION     DATAQL010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228120"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1554
                     /locus_tag="VHS97_12820"
     CDS             <1..>1554
                     /locus_tag="VHS97_12820"
                     /inference="COORDINATES: protein motif:HMM:NF019880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX3449133.1"
                     /translation="YKCWSCGAGGDVFDFVQNKERVDFPEALRMLADRAGVALESPPA
                     TAGLAERLSKTDLLLVNAWAEEIFANALADSTEAARYVESRRRTRESVNRFKLGYAPA
                     ERGWLLAQARRKKYGMEALEQAGLISQPPDSPGIWRERFRGRLIFPIHDERGRTLGFG
                     GRILPETERFLASQGRQVAKYLNSPETLIFHKRTLVYASDLARAAAREAGWVAVVEGY
                     TDVIAAHQVGAGNVVGTLGTAIGKDHQRALKHLADRVILVFDGDEAGQSAADRALEFF
                     LASNLDLRVLTLPANLDPCDFLLKEGAKAFRDLAERAVEPLDYLLTRAVGRFDLGSVH
                     GTLGAAEWVVGILSRVPESDGLELELSKSKILEKLAFRLRVPAERLNGMLRKLQRSAS
                     GQRRNGAHATRMAPGPAGGLRAANVESAPNRAQTATAAILASDLDPTDLEFIRILLNE
                     PTAFIRLIPRIGVSTLRDAPLRAILQTCYDLQADGQNPSDENVKVRLDEQAIRSLVAG
                     LAGPSAVSTL"
BASE COUNT          288 a          452 c          502 g          312 t
ORIGIN      
        1 tataaatgct ggagttgcgg agcgggcggc gatgtattcg attttgttca gaataaagaa
       61 cgtgttgact ttcctgaggc tttgcgcatg ttggctgatc gagcgggtgt tgcactggaa
      121 agcccaccag caaccgccgg cctcgcagag cgcctgtcga agaccgatct gttgctcgtg
      181 aatgcgtggg ccgaagagat ttttgccaac gcgcttgcag attcaaccga ggccgcccgt
      241 tacgtagaga gccgtcgccg gacgcgtgag agtgtgaacc gattcaagct cgggtatgct
      301 ccggcggaac ggggctggtt gttagctcag gcgcgacgca agaaatacgg aatggaagcg
      361 ctcgagcagg caggtttgat cagtcaacca ccggattcgc cgggtatctg gcgtgagcgg
      421 tttcgagggc ggttgatctt cccgattcac gacgagcggg ggagaacgct ggggttcggc
      481 ggacgaattt tgccggagac cgagcgtttt ctggcctcgc agggacggca ggtcgccaag
      541 tacctcaaca gtcccgagac gttgattttt cacaagcgaa cgctcgttta cgcgtcggac
      601 ctggcgcggg cggccgctcg cgaagcgggc tgggtggccg tggtcgaggg ctacaccgac
      661 gtgatcgcag cccatcaggt gggcgcgggc aacgtcgtag gaactttggg aacggccatc
      721 ggtaaggatc accagcgggc gctcaaacac cttgccgacc gcgtaatatt agtcttcgac
      781 ggcgatgaag cgggccaatc cgccgcggat cgtgcccttg agttttttct ggcgagcaat
      841 ctcgatttgc gcgtcctgac tctgccggcg aacctggatc cgtgcgactt tctcttgaag
      901 gaaggggcta aggctttccg cgatctggct gaacgtgccg tggaacccct cgattattta
      961 ttgactcgag ccgtcggtcg tttcgatctg ggctcggtcc acggcactct cggcgccgcc
     1021 gagtgggttg tgggtatttt gagccgcgtt cccgaatctg acggtctgga gctcgagctc
     1081 agcaaatcga aaatcctcga gaagctggcg ttccggctgc gcgtacctgc cgaaaggctc
     1141 aatggcatgc tccggaagct gcaacgctcg gcgtccgggc aacgccgtaa tggcgcacac
     1201 gccacgcgga tggcccctgg gccggccggt ggtctacgcg cggcgaacgt cgagtccgcg
     1261 cccaatcgtg cgcagacggc cacggccgcg atccttgcga gcgacctcga tcccaccgat
     1321 ctcgagttta tccgaatcct cttgaatgag ccgaccgcgt tcatacggct gatccctcgc
     1381 atcggtgtct ccactctgcg ggacgcaccg ctgcgggcga tcctgcaaac ctgttacgac
     1441 ttgcaagccg atggtcagaa ccccagcgat gaaaacgtga aggtccggct cgacgagcaa
     1501 gccatccgta gcctggtggc cggcctggcc ggcccttccg cggtaagcac actc
//
LOCUS       DATAQL010000740         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1601417, whole genome shotgun sequence.
ACCESSION   DATAQL010000740 DATAQL010000000
VERSION     DATAQL010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601417"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VHS97_12825"
     CDS             <1..1774
                     /locus_tag="VHS97_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449134.1"
                     /translation="GLDQGAAGTEARRVDRSGLAPSHAWRSVGLDFQAWPGAAGAPPV
                     FRGVALSTDSLAHDLATSPDLDALVAGRGQRGLLLTVDGEPPAMSWPVATTPLPEVPP
                     TETAAPTPISAAASPDSTRLGRIGAIAPARNLSPRGNDQRVWLTAAPLGDGVTADEPL
                     GHAVAALDPRWVFLADKAVAGQEERLRRFAAVMRSLPAWPAVAVDSQPNAGARPFGVA
                     LRRMSDDAQTFLEIANDSPYPIRLAGVLDAPASAPVDDLGRGLRLSPAPASGGRSLVL
                     DLLPYGVAGIRIGAPRVQLLSVTPYPSEAVLTIMQARFNDLSAQLARLNHGLSTTPAE
                     PPNPGFEPAAEDNPPLPVESVAKISSSAPGSKAPSADSPPLPTGWRLEEKTARSSSIA
                     IDAENPHLGHGSLRLTAPAAPASVVSERFVPNAQSSLEFQVYFRASAAGSKVRVWIDG
                     ESGGKPYVRRSELTVSTEWEPRAVRASDIPASGLDTARIRFEMMTPGVLWIDDLRVGS
                     ETPSKSARLNARITLLAALQAYREQRFADFARLAESHWIRQSSTAANNRLARANDLPS
                     KVGARPDGSSDAPASPLPSERKLR"
     gene            1941..2711
                     /locus_tag="VHS97_12830"
     CDS             1941..2711
                     /locus_tag="VHS97_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3449135.1"
                     /translation="MFVACSTLCFARQPLESALRQIAELEFDKFELALVEDGNHLRPS
                     EAGDDPEGALHRLRSGPSLIPSSLSIDFGPVDWADPVTRKRFDGLCRLAKLLSVAVLT
                     IHASPVGTPVSDEVKRLTPLVNTTMREGLVLALLTHAQTLTADPATAVELCQLLPGLG
                     LTVDPSHFLQGPNPHVDYDAVFRYAQNVHLRDTGVVPGEFQVRVGQGQIDYARIVTQL
                     QRFGYNRSLTVAIIDQPDYPFDREVELRKLKLLLETLI"
BASE COUNT          555 a         1038 c          959 g          591 t
ORIGIN      
        1 cggactggac caaggggccg ccgggaccga ggcgcgacgc gtcgatcggt ccggactggc
       61 tcccagccac gcgtggcgca gcgttggtct cgatttccag gcctggccgg gcgcagcggg
      121 tgcaccgccg gtattccgcg gcgtcgccct ctcgacggat tccctggcac acgacctcgc
      181 caccagcccc gatctcgacg cgcttgtcgc cggccgcggg cagcgcggtc tgctcctgac
      241 ggtcgacggc gaacccccgg cgatgtcttg gcccgtcgcg actactccgt tgcctgaagt
      301 tcccccgacg gaaacggccg ctcccacacc gatttcggct gccgcgagcc ccgattcgac
      361 tcgattgggc cgcatcggtg cgatcgctcc ggcgcgcaat ctaagtccgc gagggaacga
      421 ccagagggtg tggctgacgg cggcgccttt gggcgatggc gtgactgccg acgaaccgct
      481 gggacatgca gtggccgccc tcgacccccg ctgggtcttt ctggccgaca aggcagtggc
      541 cggtcaagaa gaacgcctgc gccgctttgc cgccgtgatg cgttcgctcc ccgcttggcc
      601 cgcagttgcc gttgattccc aacccaacgc tggtgccagg ccgttcggag tcgcgctacg
      661 gcgcatgtcc gacgatgcac agacatttct cgaaatcgcc aacgattccc cctatcccat
      721 ccgcttggct ggtgtgctcg acgccccagc ttcggccccc gtcgatgatc tcggccgcgg
      781 ccttcgtctc tcgccggcgc ccgcatcggg ggggcgcagc ctggttctcg acctgctgcc
      841 ctacggcgtc gccgggatcc ggatcggtgc tccgcgcgtg caactattgt cggtcacgcc
      901 ttatccgtcc gaagccgtgt tgacgatcat gcaggcgcgc ttcaacgact tatcggccca
      961 gctggcacgt cttaatcatg gactatcgac gaccccggcc gaacccccga atccgggctt
     1021 tgagcccgcc gcagaagaca atcctccctt gccggtcgag tccgtcgcca agatatccag
     1081 ttcggcgcct ggctcgaaag ctccgagcgc cgactctccg ccgcttccga cgggctggcg
     1141 attggaggaa aagacggcaa gatcgagttc gatcgcgatc gacgctgaaa atccgcatct
     1201 ggggcacggc agcctgcggc tgaccgcgcc cgcggctccg gcgagtgtgg tgagcgagcg
     1261 atttgtgccg aacgcccaat ccagcctcga attccaggtt tacttccgag cgtccgcggc
     1321 cgggtccaag gttcgcgttt ggatcgatgg agaatccggt ggtaagccgt acgtccgtcg
     1381 ctcagaactg actgtttcta ccgaatggga accgcgtgcg gtgcgcgctt cggacatccc
     1441 cgcgagcggc ctcgacacag cccggatccg gtttgaaatg atgacgcctg gcgttctctg
     1501 gatcgatgat ttgcgtgtcg gcagcgagac tccgtccaag tcggcgcggc tgaacgcgcg
     1561 gataacgctc ttggcagcgt tgcaggccta tcgagagcag cggttcgccg acttcgcgcg
     1621 ccttgccgag tcgcactgga tccgacaatc aagtacggcg gccaacaacc ggctggcccg
     1681 ggcgaacgac cttccgtcca aggtcggagc ccgccctgac ggttcgagcg acgcgccggc
     1741 ttctccccta ccgtccgaac gaaagctcag gtaaaatcgc gcggcgtcga tcgacttgcg
     1801 aaatggaagg atcgacaggc caaccctagt ggtcgttccc ccaaggcacg atctgatggt
     1861 tgtcttggtc cgccggagca ggaagggata aagcggccag gttggtcgag ccccgaggag
     1921 ggcggaagag gaggggtcgg atgttcgtcg cctgtagcac gctctgcttc gcaaggcaac
     1981 cgctcgagtc agccttgaga cagatcgccg agctggagtt cgacaaattt gagctcgcac
     2041 ttgtcgagga cggaaaccac ttacgtccgt cggaagcggg cgacgatccg gagggggcgc
     2101 ttcatcgtct tcgctcgggc ccgagcttga ttccgtcatc actctctatc gattttggcc
     2161 ccgtggactg ggcggatccc gtcacccgca aacgattcga cgggctttgt cgcctggcaa
     2221 aactgctgag cgtggccgtg ttgacgattc acgcctcgcc cgtgggcaca cccgtgtccg
     2281 atgaggtgaa acggctcacc cccctcgtca atacgacgat gcgcgagggc ctggtgctgg
     2341 cactgttgac tcacgcccag accctgacag ccgatcccgc gaccgccgtc gagctctgcc
     2401 agcttttgcc gggtttgggc ctgaccgtcg acccgagcca tttcctccag gggcccaatc
     2461 cccatgtcga ttatgacgcc gtttttcgct acgcccaaaa tgtccacttg cgtgatacag
     2521 gagtggtacc gggcgaattc caggtccgcg tcggccaggg ccagatcgat tatgcccgca
     2581 tcgtcaccca gctccagcgc tttggctata accgctcact cacggttgcc atcatcgacc
     2641 agcccgacta ccccttcgat cgcgaagttg agttgcgcaa gctgaaactc ttgctggaaa
     2701 cgctgatcta atataaatta taaagcaatc aagatcaacg caaatttccc ccccttgtga
     2761 aggggggaag gggggtggtc agcgagcaat cagccacatg tccccgaaga ggatagttac
     2821 ggggtggccc cgacacattt aattgcgggc tgttcaaacg gacgctcgcc tgtccacagt
     2881 ttggacgcgt tgtgcctggt agcgccctgg ccaccagccc aaccccccct tcacaagggg
     2941 gggaagtgct gtcttgtcca tgtcttcagc ccattactca ctgcgccaac cccagtcgat
     3001 gcaactcccg atcgagcatc tcgaggtccc agacctcgac gagaggatcc atgcccgcgc
     3061 tcgccagacg gcgattctga gtgtcaaaag ccaggctagt gatggggctc aagtgacccg
     3121 gaaggtggaa ccgcagttga ggt
//
LOCUS       DATAQL010000741         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_534604, whole genome shotgun sequence.
ACCESSION   DATAQL010000741 DATAQL010000000
VERSION     DATAQL010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534604"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VHS97_12835"
     CDS             complement(<1..1175)
                     /locus_tag="VHS97_12835"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX3449136.1"
                     /translation="MLFSRKRIRWYAREWIAAALCLTALGVAVGVFFSQSRPRRYRLS
                     ISGGNAQGLRHQIAERLAVEAVRHGLGMQIVPSSGSREVLDLVEAHTLDLAFVQGGLD
                     PAIHPHVRQIAALHVEPLHLLVKPAVYQSVSENLAALKGKTVNLGPAGSGTHDLSRDV
                     LKFAGLEPSRAHGSGDYSVLTLSYGELLTTTDSAKLPDAIFTVSALPSPVVRALAVRW
                     RYRLVPLLFGEAFALDALNRGAADAPDAEPSSSSDVAKVSIYPAVIPPFTYGIEPPTP
                     AREVATFGPRLLMVANENVPARAVRQILETVFSPPFAQISKPPLDASLLEISPEYPVH
                     AGAEQYRELNKPLMAGDVIDLLEKGTSLAGAVLGALFFLWQWMRQHFRRRRELGFES"
     gene            1466..2125
                     /locus_tag="VHS97_12840"
     CDS             1466..2125
                     /locus_tag="VHS97_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3449137.1"
                     /translation="MKSAIRRKQQQEDLRAKILDAARELFVAEGVESVSMRKIAEKIG
                     YSATTLYIYFKDKESLLHELCDRDFGTLQESFKNIGRISDPIERLRKLGQAYIAFALE
                     YPSHYRLLFMTPHDPPCGDDEYEIERGNPDQDSYAFLRATVVEALASDVFRDEYQDAD
                     LVSQVVWSALHGVASLQLIMSKDHWVQWRPVEQVAQTASDALIRGLTRTKKAKKGPGD
                     G"
     gene            2412..>3430
                     /locus_tag="VHS97_12845"
     CDS             2412..>3430
                     /locus_tag="VHS97_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3449138.1"
                     /translation="MFDDRAEMRIEETARQRGRWNPRGRQVRLVLMTWPVVALLGATV
                     TAAACLLTDCKGGKPIGAKDTLVGQSGTVKSLSFRPDGAMLASVGVDGSIVIWDLATT
                     SESALLPDVSGPVRCAVFSPNHKLLATGSPTATVALHNFDVGIGRGLDDNRAATVGAR
                     AVAFAPDGATVAVGQQDGDITLWDPATGRRRATLDGHREFVASLAFSPDGTTLASSAG
                     DRSVRIWDLPNCRQRSLLSGQSTNFADLKFSPDGLFLVLGDHVSPVVRLWDIQAGAFR
                     ESLRGPSGAVVAVAVSPDGDTLAAADFKGQITFWDFSTLKTRSKRLRHPGVLALAFAP
                     DGRTLA"
BASE COUNT          701 a          988 c         1066 g          675 t
ORIGIN      
        1 tacgactcaa agccgagctc tcgcctgcga cgaaaatgct ggcgcatcca ttgccacaag
       61 aagaacagcg cgcctaaaac cgcgcccgcc aggctggttc ctttctcgag caggtcgatc
      121 acgtcgcccg ccattaacgg cttgttgagc tcccgatatt gctcggcccc ggcgtgcacg
      181 ggatattccg gcgaaatttc gagcaagctt gcatcaagag gaggcttgga gatctgagcg
      241 aacggaggtg agaaaaccgt ctccaggatc tgccggactg cgcgagccgg tacgttttcg
      301 ttggcgacca tcaaaagccg aggaccgaat gtggcgactt cgcgggcggg tgtgggcggc
      361 tcgatgccgt acgtgaaggg agggatcaca gccgggtaaa tgctcacttt cgccacgtca
      421 ctactggaac tgggctcggc gtcgggtgca tcggctgcgc cacgattgag cgcatcgagt
      481 gcgaacgcct cgccaaaaag gagcggcacg agccgatagc gccagcggac cgccagggct
      541 cgcaccaccg gcgagggcaa cgcggagacg gtgaaaatgg catcggggag cttggcggag
      601 tcggttgtgg tgagcagctc cccgtagctc agggtcaaaa ccgaataatc acctgagcca
      661 tgagcgcggc tgggctcgag gcccgcaaat ttgaggacgt cgcgcgacag gtcgtgcgta
      721 ccactcccgg cgggaccgag gttgacagtc ttgcccttga gtgcagccag attttctgag
      781 accgattgat aaacggccgg cttgaccagc aaatgcaacg gctcgacgtg aagcgcggca
      841 atttgccgca cgtgcgggtg gatcgcgggg tcaagccctc cttgcacgaa agccagatcg
      901 agggtgtgcg cttctacaag gtcgaggact tcgcgggatc ccgaactcgg cacaatctgc
      961 atgcccaatc cgtggcggac cgcctcgacc gcgagccgct cggcgatctg atgccgcagc
     1021 ccttgggcgt ttccccccga aatcgacagc cgataccgcc gcggtcgcga ctgcgagaaa
     1081 aataccccca ccgccacgcc cagggcggtc agacacaaag ccgcggcgat ccactctcgg
     1141 gcataccagc gaattcgctt gcgagaaaaa agcatcaccc aatcccggcg gttcgcgctt
     1201 attttttggg acgctctacg tcgcgaatca gaatctcatt attcccgcat cgccgcgacc
     1261 cccgccctgg gatttcctct tcagttaatc gacccgattg atagactctg gatgttattc
     1321 gtacgctcga aatggttctt gcacaagcga atcggtcgtt accaccctct ttactcgaca
     1381 cgcaacacca gaatcaagcc actcgttgct tgacatcgat aacgctgtta actaagataa
     1441 caccgttcat tagataggaa cgaggatgaa atcggcgatc agacgcaagc agcaacaaga
     1501 agacctgagg gcgaagattc tcgacgcagc acgtgaactg ttcgtcgcgg aaggtgtgga
     1561 gtcggtctcg atgcgcaaga tcgctgagaa gatcggctac tccgccacaa cgctctacat
     1621 ttatttcaaa gacaaagagt cgctgttgca tgagctctgc gaccgagact tcggcactct
     1681 tcaagagtcg ttcaagaata tcgggcggat cagcgatccg atcgagcggc tccgaaagtt
     1741 gggacaggct tacattgctt ttgcgctcga gtatcccagc cattaccggt tgctgttcat
     1801 gaccccgcac gacccgccgt gcggcgatga cgagtatgag atcgagcgtg gcaaccccga
     1861 ccaggattct tacgcttttt tgcgggcgac cgtggttgaa gctcttgcgt ccgacgtgtt
     1921 tcgcgacgag taccaggatg ccgatttggt ctcgcaggtg gtctggagcg cgttacatgg
     1981 cgtggcgtcg ttgcagttga ttatgagtaa agaccactgg gttcagtggc ggcccgtcga
     2041 gcaggttgcg cagacggcga gcgacgcctt gatccgaggc ttgactcgga ccaagaaagc
     2101 caaaaaagga cccggcgacg gttgatctga gcggacggcg aacctcgacg cccacttggg
     2161 catccgatac taaaattggt cgaccacggc aactccaagt cgactggttc gcgttaaagc
     2221 gacacatgat tggaagttat catgtcattg cttgatgcat cggcgccgag tccccgcgcc
     2281 gaaaccgaga atcatttgtt catccaagag cgctcaatat tgcgatatga ctcacgaata
     2341 cgagggtgga gagacaactc tcgggcgggg cagagtcaag tggcccgtcc gttgtcggag
     2401 gattgacggt gatgtttgac gatagggccg aaatgcgcat tgaagagacc gctagacagc
     2461 gtgggcgatg gaatccaagg ggacggcagg tccggctggt actcatgaca tggccggtgg
     2521 ttgcgctctt gggggccact gtcacggccg cggcatgcct cttgaccgac tgtaaaggtg
     2581 gcaagccgat tggggcgaag gacacattgg tcgggcaatc cggcacggtc aagtcgctgt
     2641 cgttccggcc tgacggggcg atgttggcgt ctgtgggagt cgatggctcg atcgtgatct
     2701 gggacctggc aaccacttcg gagagtgcgc ttctgccgga tgtgtctgga ccggtgcgct
     2761 gtgcggtctt cagcccgaac cacaagttac tggcgacggg tagcccgacg gcgacggttg
     2821 ccctgcacaa tttcgacgtc ggtatagggc gcggactcga cgataacagg gccgcaaccg
     2881 ttggcgcgcg ggcggtggcg tttgcgcctg atggcgcgac cgtggctgtg ggacagcagg
     2941 atggcgatat cacactctgg gaccccgcca ccgggcgcag acgggcgacg ctcgatgggc
     3001 atcgggagtt tgtcgcctct ttggctttct cgcctgatgg taccaccctg gcctcctccg
     3061 ccggcgatcg ttcggtgcgg atctgggatt tgccaaactg ccgccaacgg tcgttgctga
     3121 gcggtcagtc aaccaacttc gctgacctga aattctcgcc tgacggccta ttcttggtgc
     3181 taggcgacca tgtgagcccg gttgtgcggc tgtgggatat acaggcgggg gcattccgtg
     3241 agtctcttcg cggaccgtcg ggtgcggtgg tcgcggttgc ggtcagcccc gacggggaca
     3301 cccttgccgc cgcggacttc aaaggacaga tcaccttctg ggacttttcg acgctcaaaa
     3361 cccgctcgaa gcggctgcga caccccggcg ttctcgcact tgccttcgct cctgacggcc
     3421 gcacccttgc
//
LOCUS       DATAQL010000742         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4348983, whole genome shotgun sequence.
ACCESSION   DATAQL010000742 DATAQL010000000
VERSION     DATAQL010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4348983"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VHS97_12850"
     CDS             <1..276
                     /locus_tag="VHS97_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449139.1"
                     /translation="SRYTIRDGDRRLELSLAPRADQNDGLVALASIVSKTVRELWMDV
                     FNAYWRRQIPELRPTAGYPNDSLRFRREIENLAAAQGHDPARWWRIR"
     gene            complement(326..544)
                     /gene="infA"
                     /locus_tag="VHS97_12855"
     CDS             complement(326..544)
                     /gene="infA"
                     /locus_tag="VHS97_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX3449140.1"
                     /translation="MPKEEPIRTEGRIVEALPNTQFMVELENGHRILAHIAGKMRKNF
                     IRIVPGDRVTVEITPYDLSKGRIVYRER"
     gene            complement(819..1712)
                     /locus_tag="VHS97_12860"
     CDS             complement(819..1712)
                     /locus_tag="VHS97_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3449141.1"
                     /translation="MATIKRDAGLRALVESEEVERLADGFQFTEGPLWQPDGSLLFQD
                     IRAERTYRLDPDRSVHLVREQTRAANGQTFGPGASIVFCEQNGRRLSRFDLADCKVEP
                     LVETWNGARLNSPNDVVSRSDGLIYFTDPAYGVEPEERALHFQGVFVLDPRAHGQEAL
                     RLVADDFEKPNGLAFSPDERTLYVCDTGRYHVRAFAVEPDGSFGVGSSRVFAKLDPGE
                     PGGPDGMKVDRAGRVYVAVALGIWVFEPDGRLLGILSMPVRPSNLAWCDSDARGLAIT
                     AVDAVFHVRLRVEGIMPPFMP"
     gene            1992..3386
                     /locus_tag="VHS97_12865"
     CDS             1992..3386
                     /locus_tag="VHS97_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449142.1"
                     /translation="MSSDAEKPDLKNAQTFFQYGNDAALKQNFDYAIEMYKRACKIVP
                     DNLVYRQALRGIERRKFNGDPSKVGMLVGAKNQPLLMRAKSAKSKGNHTQAIEHCEDA
                     FVNNPWDVGAARVAAEAAEQAGFLVLAQWFVESVQTNSKDVDFFKFSAHIHEINESWQ
                     KAIACWEHVKKLSPNDQEASRTINALSAAGTIKRAGLEDTLDQRAHAAANPEPAESMD
                     DQLKRLKQAQLSPEQRLVKEIITDPTAVHAYMDLAEIYRQHGELDKAEKVLAKGRKAN
                     PDDPGLASTYEDTQISRLKKARDSQSQRVLQYPEDTGAKAKLDQLTEMLNKYEIEAFR
                     RRVNLHPEDAKLHLDLGIILARVGDHDGAIAEFQHARASTSPTQRIQALYNLGLSFEA
                     NSSPKLAERNYKEALKLLEPDDKENLLKLHYRLGRVAETLGNTEAADNSYNEVAAIDY
                     GYLDVAERLKRLNS"
     gene            3544..3825
                     /gene="rpsT"
                     /locus_tag="VHS97_12870"
     CDS             3544..3825
                     /gene="rpsT"
                     /locus_tag="VHS97_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX3449143.1"
                     /translation="MPNSTSAAKRMRQSVKRRKLNRVTKKVIKTLTKRTLASLASKEF
                     DKAATDIKEASSKIDKAGARRVLHPNTAARRKSKLARAYKAALAKSGQA"
BASE COUNT          912 a         1217 c         1205 g          711 t
ORIGIN      
        1 agccgttaca cgatcaggga cggcgaccgt agacttgagc taagcctggc ccctcgtgcc
       61 gaccagaacg atggtctggt cgcgctggcg tcgatcgtca gcaagacggt tcgggaactc
      121 tggatggacg tcttcaacgc ctactggcga cggcaaatcc cagagcttcg accgacggcc
      181 ggttatccga atgattccct tcggttccgc agagagatcg agaacctcgc ggcggcccaa
      241 gggcatgacc cggcccgctg gtggcgcatc aggtaaggct gtcgccgatg tcaaagttcc
      301 atctctggcg atcgggcagg tcaattcagc gctcgcggta gacgatacga cccttggaga
      361 ggtcgtacgg tgtgatttcg acggtcacgc ggtcaccggg cacaatgcgg atgaaattct
      421 tgcgcatttt gccggcaatg tgggccagaa tgcggtgtcc gttttccaac tcgaccatga
      481 actgcgtatt gggtagcgct tcgacgatgc gcccctcggt acgaatcggt tcctctttgg
      541 gcacgcggat ctcctgaagt cgaccagcgc cggccgcgtc gttggtctgt tcgtgtctat
      601 acaaaattgc cacgccgacc ggtctcagca cgctgcgcca cgcgcgaacc caggcgggac
      661 gactgccgct ggagcccgcc ggagggccgg atcgaaacgt gtgagattcg cctgagtctt
      721 gagaataatg cattacgatg gactcgaaca gtagacccgg cgaaagaaat cagcaattac
      781 gggccagcgt ccggcaaacc tgacccgaca atcgcggttc acggcatgaa cggcggcatg
      841 atcccttcca cccggaggcg cacgtgaaac accgcgtcga ccgcggtgat cgccagacct
      901 cgcgcatcgg agtcgcacca ggccaggttt gacggccgaa ccggcatcga cagaattccc
      961 agcagccgtc cgtccggctc gaacacccag atcccgagcg cgacggcgac gtaaacccgc
     1021 ccggcccggt caaccttcat gccgtcgggg ccgccgggtt caccagggtc gagcttcgca
     1081 aaaacacggc tcgagcctac gccgaacgaa ccgtcgggtt cgacagcgaa ggcccggacg
     1141 tgatagcgac cggtgtcgca cacgtaaagc gttcgctcgt cgggtgaaaa cgcgaggccg
     1201 tttggtttct cgaaatcatc cgcgaccaag cgaagggcct cctgtccgtg ggcacgagga
     1261 tcaagcacga aaacaccctg gaaatggagc gcgcgctctt ccggctcgac cccgtaggca
     1321 gggtcagtaa agtaaattag accgtcggac ctgctgacga cgtcattggg gctattgagc
     1381 cgcgcgccat tccaggtctc gaccaatggc tctactttgc agtcggccag gtcgaatcgt
     1441 gatagccggc ggccgttctg ctcacagaaa acgatcgaag cgccggggcc aaaggtctgc
     1501 ccattggcgg ctcgtgtctg ctcacgcacc agatgaaccg agcgatccgg atcgagccgg
     1561 taggtccgct cggccctgat atcctgaaaa agtagcgagc catcaggctg ccagagcggc
     1621 ccttcggtaa actgaaagcc gtcggcgagc cgctcgacct cctcactttc gaccagcgcc
     1681 ctcaggccgg cgtctctctt gatcgtcgcc atcgctcata tctcccacaa aaataggcga
     1741 attcgacttg cttgcaagct cgccgccggt ttgaatcata agtcgccggt ggtgatcggc
     1801 accatgttgg attagaatag attggcgaca tgttgcgggc catgggtgtt cccacgagca
     1861 cccgcggtgc cggctgcccg tcgagcgtgt cccctaatgc ctggatccat cgtataagaa
     1921 aagacgagcc acggtcggtc ccgcgttgtt cacgaagcgg tcgattcttg gtttgcggag
     1981 gagcgccgtc gatgtcttcg gacgccgaaa aacccgatct gaaaaacgcc caaacgttct
     2041 tccagtatgg caatgacgcc gcccttaagc aaaatttcga ctatgccatc gaaatgtaca
     2101 agcgtgcgtg caagattgtt ccggacaatc tggtttatag gcaagccttg cggggtatcg
     2161 agcggcgcaa gttcaatggc gatccgagca aggtagggat gctggtgggg gcgaagaacc
     2221 agccgctcct gatgcgcgcc aagtcggcga aatcgaaggg gaatcacacg caggcgatcg
     2281 aacactgcga ggatgcgttt gtcaataatc cctgggacgt cggtgcggcg cgagtcgcgg
     2341 ccgaggcggc cgagcaagcc ggttttctgg tcctggcgca gtggttcgtc gagtcggtac
     2401 agaccaactc caaagacgtc gactttttca agttctccgc ccacattcat gagataaacg
     2461 agagctggca aaaagccatc gcgtgctggg aacatgtcaa gaagctgagc ccgaacgatc
     2521 aagaagccag tcgtacgatc aatgcactgt cggcggctgg tacgatcaaa cgtgcaggcc
     2581 tcgaggatac actcgatcag cgtgcccacg cggccgccaa tcccgagccg gccgaatcga
     2641 tggacgacca gctcaagagg ctgaagcaag cgcagctctc gccggaacag cggctggtca
     2701 aggagatcat caccgacccg acggcggtgc acgcctacat ggatctggcg gaaatctacc
     2761 gccaacacgg cgaactcgac aaggcggaaa aggtccttgc caagggccga aaagccaatc
     2821 ccgatgatcc cgggctggca tcgacctatg aagacacaca gatcagccgt ttgaagaaag
     2881 caagggatag tcagagccag cgggtgttgc agtatccgga ggacaccgga gccaaggcca
     2941 agctcgacca gttgaccgag atgctcaata agtacgagat cgaggcgttc cgccgccgcg
     3001 tgaacctgca ccccgaggac gcgaaactgc atctcgatct gggcataatc ctggcgcggg
     3061 tcggtgatca cgatggggcg atcgccgaat tccagcacgc gcgggcaagc acaagtccca
     3121 cgcaaaggat ccaggcactc tataatctgg ggctgagctt cgaggcgaac agttccccca
     3181 agctggcaga gcgcaattac aaggaggctc ttaaattgct cgagcccgac gacaaagaaa
     3241 acttgctcaa gttgcattac cggctgggtc gcgtggccga gacgctgggc aataccgagg
     3301 cggccgacaa cagctacaac gaggtcgccg ctatcgacta cggttatctc gacgtggcag
     3361 agcgtctcaa acgcctgaac tcgtgaaacg cttgaactca agctcgtgcg tatgtaaaat
     3421 aatcatgcct tgagcgggcc ggactttgcg tccgcgtcgc gtgattcgaa ggtcgaaagg
     3481 ccggtgctct acgtagaccc gggctttgtt gcctccaagt ttctcctaac gatcaacgga
     3541 gctatgccaa attcaacctc cgcggccaaa cgcatgcggc agagcgtcaa gcgccggaag
     3601 ctcaatcgcg tgaccaaaaa ggtcatcaaa acactcacga agcgtacctt ggcatcactg
     3661 gcgtcaaaag agttcgacaa ggcggcgacc gacatcaagg aagcgtcgtc caagatcgac
     3721 aaagccggcg ctcgtagagt cctccatccc aacacggcgg cccggcgcaa gagcaagctg
     3781 gcgcgggctt acaaggccgc attggccaag tcagggcaag cctgaatccg cactcgaggg
     3841 atcgattggc ggcacgagcg tgactgcggg ccaagacctc tctttcggtc ttcgagccgg
     3901 caagaggcca acaccccgcg agggataccg ccaccatgcc gaccctcgac gactcttttg
     3961 aagcggttca caccgcgctg ctcgaccgtt tcggcccgcc gccgaccgaa ttcgagggct
     4021 tggacccgtt cgaagccatg gtcgc
//
LOCUS       DATAQL010000743         7582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4808615, whole genome shotgun sequence.
ACCESSION   DATAQL010000743 DATAQL010000000
VERSION     DATAQL010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4808615"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VHS97_12875"
     CDS             complement(<1..1316)
                     /locus_tag="VHS97_12875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449144.1"
                     /translation="MAWEFWRRPNGDGNLTVIDIGTTLNQRFFLDKELGRGGMGAVYS
                     ATDQILERKVAIKVLKEQSGEEVGKRLRLEAQIAARLLHDNVVRIYDFGRAEDAYFLV
                     MEEVSGTSYAKRWRLLSLPDRLSVLADVADALDYAHHQGVVHRDVKPANVLLTTSDVP
                     KLSDFGLSIIAARGDQTGVVRGTPHYMSPEQTRGSRLDYRTDLYSLGVMVYESATGAV
                     PFTGTSVSVMSQHYQTNPERPRARNPFVSPELEALILALLAKRPDERPGSGALAALAL
                     RQEVARIRERDGSGGSAATASVGVGVAGATNLTGTVGLTAPVATEPLPAQSIQPANGL
                     TATRTSSAFSSSSPSDTISGETLVTGAGPPAPTIPSSPSKHPQEGGPSSLAPVAIATV
                     GGTGGVTALIRSPLARRMLEVVLAEPILLSPDERYLHGHYLAYLMSG"
     gene            complement(1486..2253)
                     /locus_tag="VHS97_12880"
     CDS             complement(1486..2253)
                     /locus_tag="VHS97_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449145.1"
                     /translation="MTPESGSTTRRDFARNALQSLTAFVLVEGLWSRRLLGEDVKPIV
                     DAWFSELYAICRDVHQHRNKDIEFQGALERLYQQVDLKALMKTLDFDRLARGINFPSK
                     GAKSLPVDFAHVGGLPVKLPFGGQIFAVGKGRSVVPHGHDNMATGFVVLKGDFRGRHY
                     DRIEDHADHYIIRPTIDRAFQTGEFSTISDHKDNVHWFTAENETGFLFNIHVVETNPE
                     NPKRPGRVYVDPMGEKLAGGLIKAAKLSYGRVNQLYG"
     gene            2505..2813
                     /locus_tag="VHS97_12885"
     CDS             2505..2813
                     /locus_tag="VHS97_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449146.1"
                     /translation="MAGFGRFKLTDALPEQAADVEPSQFPGGGHAASAAGKGKGKGKG
                     RGKTASAKGKTALGSGLAAEQLREARESNQAKNAEGSIRDRMVDIGRGNQQAGRSGHK
                     "
     gene            2895..3737
                     /locus_tag="VHS97_12890"
     CDS             2895..3737
                     /locus_tag="VHS97_12890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3449147.1"
                     /translation="MDLGLAEQVVVVFGAARGIGAAIATAFAAEGAHVTAVDRDPSVH
                     QLAQALPRSLSVVADVTNLTAVRQAAQEVEDRFGRCDHVVFAVGAGSGKFGFPFWKLE
                     PADWDPVLKINLIGAVNVAHVVAPTMATAARSSRESEASNTRISNSSRSILFLSSVAG
                     QIGSQSDPPYSAAKAALINFAQCAAKDLAPFGVRVNTLCPGMVQTPLNRGVYEAWARE
                     QPESARSTYDAWTADKIKKLVPLNRWQQPEDIAAMAVFLASSQAVNITGQTVNVDGGY
                     VMHW"
     gene            3881..4786
                     /locus_tag="VHS97_12895"
     CDS             3881..4786
                     /locus_tag="VHS97_12895"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HEX3449148.1"
                     /translation="MARITLGAERFDTEASGPEYRGLEAGDILYFPVSPPLLPDEDRA
                     FLVTQKQVDASYHKNISYRPLEDRLKGVDQADSAERARVHEIMRGYSQRAVAFTKTFL
                     KRYAANWKIDFASFRPIEESGRKVALHSRNDLLHFDSFPTRPSHGDRLLRIFTNIHPE
                     RERVWLTTDHFETLAGQFAERIGLYRKQGALGRWKHLTAQAVAGLGLPFVARPAYDRF
                     MLKIHHAMKEDAGFQKTCRKDRWEFPAGSTWIVFTDSASHACLSGQYALEQTFIIRRA
                     SLAAPDKAPITILEKLAGYPLARSA"
     gene            5043..>7582
                     /gene="uvrA"
                     /locus_tag="VHS97_12900"
     CDS             5043..>7582
                     /gene="uvrA"
                     /locus_tag="VHS97_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX3449149.1"
                     /translation="MAATDIVIRGAREHNLRDVSLVLPRGKLICLTGVSGSGKSSLAF
                     DTLYAEGQRRYVESLSSYARQFLGQMPKPEVDRIDGLSPSISIQQKTGGRNPRSTVGT
                     ITEINDYLRVLYARIGQGHCPNCGRPVTAQTREQIVTRVLEELPAGTGFLVLAPVVRG
                     QKGEYKDLFADLSRAGFVRARVNGQVVNLSDDLSLDRQIKHQIEAVIDRLKAVPDPSS
                     TQRGRLAEAIEQALALGEGTVVIAAEGRPDWLLSSNYSCVHCGIGFDPPSPQLFSFNS
                     PQGMCLACDGLGIRHDFDPDLLVPNASLSVWDGAIAPLGPVKQIGRWRRHLFEGLAAN
                     LEADPDGPAKGTMLKGPWRNLDEKSRHAWLYGTGDRQIVHRWKSRHKVWSHAEKWAGV
                     ANELLAKYRGVSGGPLRAQLDPFMRSMTCPECSGTRLNPRARAVKVGGRTLVEVGAMP
                     IGQVAHFFNALADLAPGDNSSSVDVIPLDAVSRTIGEELLKEIRARLTFLIDVGLHYL
                     ALDRAAPTLSGGEAQRIRLASQVGAGLVGVLYILDEPSIGLHPRDNDRLLATLRRLAQ
                     LGNTVVVVEHDEDTMRAADHLVDFGPGPGVKGGEVVAQGTIDDLARTRESLTGAYLAG
                     KRAIAIPAERKIPDGRFLTIKGATHNNLKDIDAAFPLGLFICVTGVSGSGKSSLVGDI
                     LREALARELNRAVTDPGAHAGIEGLEHLDKVIDIDQSPIGRTPRSNPATYIKLFDLIR
                     DLFTRLPEAKARGYAPGRFSFNVSGGRCEACEGNGSNRLEMDSLADVWVTCPVCQGKR
                     FGRETLQVRFKGKSISDVLDMDVQEALEHFANIPKIAVMLQTLHDVGL"
BASE COUNT         1481 a         2284 c         2320 g         1497 t
ORIGIN      
        1 ccgctcatca agtaggcgag atagtggcca tgaagatagc gctcgtcggg agatagtaga
       61 atcggctcgg ccaggacgac ctcgagcatc cggcgggcca gtggcgagcg aatgagcgcg
      121 gtcacacctc cggttccgcc aacggtcgca attgcgacgg gcgcaagtga tgaagggccg
      181 ccctcctgtg ggtgtttcga tggggaggaa ggaatggtcg gcgcgggtgg gccagcgcca
      241 gtcacgagcg tctcgccact aatcgtgtcg ctcggtgaag aagagctaaa tgcgctggag
      301 gtccgtgtcg ctgtcaaccc gttggccggc tggattgatt gcgcgggcaa aggttcggtc
      361 gccacgggtg ccgtcaagcc gaccgtccct gtcaaattcg tagcgcccgc gacaccgacg
      421 ccgacggatg ctgtcgccgc actaccgccg gatccatcgc gttcacgaat ccgcgcgacc
      481 tcctggcgca gcgccagagc agccagtgct ccggatccag gccgctcgtc gggccgcttt
      541 gccagaagcg ccaggatcag agcctcgagt tcgggcgaga caaaaggatt gcgcgcccga
      601 ggcctttcgg gattagtctg gtagtgctga gacatgacgg agacagacgt ccccgtaaac
      661 ggcacggcgc cggttgccga ctcgtagacc atcaccccaa gcgaatacag atccgtacga
      721 tagtcgagcc ggctcccgcg cgtttgctcc gggctcatat agtgcggcgt gcctcgtacg
      781 acccctgttt gatctccgcg ggcggcgatg atcgagagcc cgaagtctga cagtttgggt
      841 acgtcgcttg tcgttaaaag cacgttggcc ggcttgacgt cgcggtggac cacgccttgg
      901 tggtgtgcat aatcgagcgc gtcggccaca tcggcaagca cgctcaggcg gtcgggcagg
      961 gacaaaaggc gccaccgttt cgcatagctg gtcccgctca cctcctccat taccaagaaa
     1021 taggcgtcct cggcccgccc gaagtcgtag atccgcacga cattgtcgtg cagcagccgc
     1081 gcggcgatct gcgcctccaa acgcagcctt ttgcccacct cttcaccgct ctgttccttg
     1141 agaaccttga tggcgacttt gcgttccagg atctggtcgg tggctgaata gacggctccc
     1201 atgcctccgc ggccgagttc cttgtcaagg aaaaacctct ggttgagcgt ggtaccgata
     1261 tcgatcaccg tgaggttccc gtcaccattg ggtcgtctcc aaaactccca cgccatgtcg
     1321 cgattcgtcg tgccgcggtt tatgcacgcg cctgcactca ggccgaaaag cctttgtcac
     1381 ccgcgtgtaa gaataatgct aagatatggc gaagactgac acaacgatcc atttcgatcg
     1441 gtccgaatgg gatccatgcg gcacccctcg gtctgttgcg atcgcctagc cgtagagttg
     1501 attgaccctt ccgtacgaca gcttcgcggc tttgatcaag ccgccagcga gcttttcacc
     1561 catggggtct acgtaaactc ggccgggccg cttcgggttc tctgggtttg tttccactac
     1621 gtggatgttg aacaggaatc cggtttcatt ttcggccgtg aaccagtgga cgttgtcctt
     1681 gtgatcggag atggtcgaga attctcccgt ttgaaacgcg cggtcgatcg tgggccggat
     1741 gatgtaatga tcagcgtggt cctcaatccg gtcgtagtgc cgcccgcgaa agtctccttt
     1801 cagaaccacg aagccggtgg ccatgttgtc gtggccatgg ggtacgaccg agcgcccttt
     1861 acccacggcg aagatctgcc cgccgaatgg tagtttgact ggaagaccgc cgacgtgagc
     1921 gaaatcgacc ggcaaactct ttgccccttt cgacgggaag tttatccctc gagccagccg
     1981 atcgaagtcc agcgttttca tcagggcttt caagtccacc tgctggtaaa ggcgctccag
     2041 agcgccctgg aactcgatgt ccttatttcg gtgttggtgc acatcccggc agattgcgta
     2101 aagctcgctg aaccaagcgt caacgattgg cttgacgtct tcaccaagga gccgccgcga
     2161 ccagagaccc tcgaccaaaa caaacgcggt cagcgactgc aaggcattcc gtgcgaagtc
     2221 gcggcgcgtt gtcgatcccg attctggcgt catgagaggt ctccggagta acagtgtggt
     2281 ggacgaacag caatgactgg cgccggctgt caaagtggga atgcgtcgga acacgtgagg
     2341 taggacattg cagccagtct agcgtgggtc ataggctatt ttccagtcgc cattggcgag
     2401 cgcagattgg aggcaagcgg caagttgcta gtgaaaacca aacccatttc ggctttacta
     2461 gcaagcaggt gcaatccaat ttgaaatcga gggagataac gacgatggcg ggatttggac
     2521 gcttcaaact caccgatgcg ctgccggagc aagccgcgga cgtcgaaccg tcgcagttcc
     2581 ccggcggggg ccacgcagcc tcggctgccg ggaagggcaa gggtaaaggc aaagggcgag
     2641 gcaagaccgc cagtgccaag ggcaagaccg cactggggag cggtctcgcg gccgagcagc
     2701 ttcgtgaggc gcgcgagagc aatcaggcca agaatgccga aggatcgatt cgggatcgga
     2761 tggtcgacat tggccgtggc aaccagcaag cgggccgctc aggccacaaa tgagcatgga
     2821 atgccgatcg gtcgacgagc aagtccctga ccgttgcaac agtcgagagc cttgaattca
     2881 aggagccgag cgcagtggat ttgggactcg cggagcaagt ggtcgttgtc ttcggcgcgg
     2941 ctcgtgggat cggcgcggcg atcgcaacag cctttgcggc cgagggcgcg cacgtcacgg
     3001 ccgtcgatcg cgacccgagc gttcaccaac tcgcccaggc acttccgcgg tctctgagcg
     3061 tggtggcgga cgtgaccaac ctcaccgctg ttcgccaggc cgcccaagaa gttgaggatc
     3121 gctttgggag gtgcgaccac gtcgttttcg ccgtcggcgc tggttccggc aaattcggct
     3181 ttccgttctg gaagctcgag ccggccgatt gggatcccgt actgaaaatc aacctgatcg
     3241 gcgcggtgaa cgtggcacac gtggtcgccc ctaccatggc gaccgcggca cgctcaagca
     3301 gagagtccga ggcgtccaac acccgaatca gcaactcgtc gcgctcaatc ctttttcttt
     3361 cgtcggtcgc cggccagatc ggctcgcagt ccgaccctcc gtacagtgct gcaaaggcgg
     3421 cgttgatcaa cttcgcccag tgtgccgcga aagacctcgc ccctttcggc gttcgggtca
     3481 acacgctctg tcctggaatg gtccagacgc cccttaatcg tggcgtctat gaagcttggg
     3541 ccagagagca gcccgaatcc gcgcggtcaa cctatgacgc ctggacggcc gataagatca
     3601 aaaagctcgt cccgctcaac cgctggcaac aacccgaaga cattgcggcc atggccgtct
     3661 tcctcgcatc ctcccaggcc gtcaacatta ccggccagac ggtgaacgtc gacggcggct
     3721 acgtgatgca ctggtagtcc ctcatccatc ttgagcaggc aactcgtgaa cttctcggcg
     3781 cttcgcgggc gcaacgggtt cgtctttcct gatttgccga tcgaggtatg atcgcggcag
     3841 catgcagtag gtaggaggca gtatcaagga gtgatcgagc atggcacgga ttacgcttgg
     3901 tgcggagcgg ttcgacacgg aagccagtgg gcccgaatat cgcggactcg aagctggcga
     3961 tatcttgtac tttcccgtgt ctccaccgct cttgcctgac gaagaccggg cctttttggt
     4021 gacccagaag caggtcgatg cttcgtacca caagaatatc agttaccgcc cgctggagga
     4081 tcgattgaaa ggggtcgatc aggccgactc cgctgaacgt gcacgcgttc acgagatcat
     4141 gcgtggttac tctcaacgtg ccgttgcgtt cacgaagacc tttttgaagc gctacgcagc
     4201 gaactggaag atcgacttcg cgagctttcg acccatcgag gaatcgggtc gcaaggttgc
     4261 cttgcactct cgcaatgacc tgctgcattt tgactcattt cccacgcgcc cgtcgcacgg
     4321 tgatcgattg ttgcggatct tcactaacat tcacccagaa cgggagcggg tctggcttac
     4381 aacggaccac tttgagacct tggcaggcca gtttgcggag cggatcggac tgtatcggaa
     4441 gcagggcgcc ttgggccgct ggaaacacct gacggcccaa gccgtggctg gtctgggcct
     4501 gccctttgtc gctcgtcctg cttacgaccg ttttatgctc aagattcacc acgccatgaa
     4561 ggaagacgcc ggctttcaaa agacttgtcg caaagaccgc tgggagtttc ccgccggctc
     4621 gacatggatc gttttcaccg acagcgccag ccatgcctgc ttgagcggcc agtatgccct
     4681 ggaacagact ttcatcatcc gtcgggcgag cctcgcagcc cctgacaagg ccccgatcac
     4741 gatccttgag aagctcgcgg gttatccgct ggcgcggagc gcgtaaacgt ccttgaagat
     4801 cctcaatccg gttcgacgcg ccttctttat ttaaatgctg gctcgcaccc ggcctccgcg
     4861 agcgttacct cctgcgcgtg tggaacaaga agtggcagcg ccatgagagc ctgtcatctc
     4921 ggctaaccag cgcctgtaca ccctaacgcg acacgtgtat agtaatcgtt tctcaacttt
     4981 cggatcgaca accacgacgg tcgcggattc caggccgcac cgtctaatgg agacaactag
     5041 ggatggccgc gaccgacatc gtgatccgcg gcgcgcgcga gcataaccta cgcgatgtgt
     5101 cgctggtctt gcctcggggt aagttgatct gcctgacggg cgtgtcgggc tccggtaaga
     5161 gttcgctcgc atttgacacg ctttacgcgg aagggcagcg ccgttatgtc gagagccttt
     5221 ccagctacgc tcgccaattc ttgggccaga tgcccaaacc cgaggtcgac cgcatcgatg
     5281 gcctgagtcc ctcgatttcg atccagcaga aaactggggg ccggaacccg cgatcgacgg
     5341 tcggcacgat taccgagatc aacgactatc tccgtgttct gtacgcccgc atcggccagg
     5401 gacactgccc gaattgtggc cggccggtga cggcccaaac gcgggaacag atcgtcacgc
     5461 gggtgctgga agagttgccg gccgggacag gctttctcgt cttggcgccg gtcgtgcgcg
     5521 gccagaaggg ggaatacaaa gacctctttg ccgatctgtc ccgagctgga tttgtacggg
     5581 cgagggtcaa cggccaggtt gtcaatctgt ctgacgacct atcgctcgat cgccagatca
     5641 agcatcagat cgaggcggtc atcgaccggc tcaaggcggt tcctgatccc agttcaaccc
     5701 agcgcgggcg tttggccgag gccatcgagc aggccctcgc gctcggcgaa gggacagtcg
     5761 tcattgccgc cgaggggcgg ccggattggc tgctctcctc gaattattcg tgcgtccact
     5821 gcgggatcgg cttcgatccg cccagtccgc aacttttcag ctttaatagc ccccagggga
     5881 tgtgcctggc gtgcgatggc ctgggtattc gccacgattt tgaccccgac ctcttggtcc
     5941 ccaacgcctc actctcggtt tgggacgggg cgatcgcgcc ccttgggcca gtcaagcaga
     6001 ttgggcggtg gcggcggcat ctcttcgagg gacttgcggc caatctcgag gccgacccgg
     6061 atggccctgc caagggaacg atgctcaagg ggccgtggcg aaatctcgat gaaaaatcgc
     6121 gccacgcgtg gctctatgga accggcgacc ggcagattgt ccatcgttgg aaaagtcgac
     6181 ataaggtttg gtcccacgcc gagaaatggg cgggtgtcgc caacgagtta ctggccaagt
     6241 accgcggtgt ctccggcggc ccgctccgcg cgcagctcga cccgttcatg cgcagcatga
     6301 cctgccctga gtgctcgggt accaggctta acccccgcgc gcgagcggtc aaggtcggcg
     6361 gccggacctt ggtcgaggtg ggggctatgc ccatcggcca ggtcgcccac ttcttcaatg
     6421 ctctggccga cctagcaccg ggtgacaaca gctcgtcggt cgatgtcatt ccactcgatg
     6481 ccgtctcacg gacgattggc gaggagttgc tcaaggagat tcgtgcgcgg ctcacattct
     6541 tgattgacgt cggcttgcac tacctggccc tggatcgtgc cgcgcccacg ctctcgggcg
     6601 gcgaagcaca gcggatccgc ctggccagcc aggtgggcgc cgggctggtg ggagtgcttt
     6661 acattctcga cgagccgtcg atcggcttgc atccgcgtga caacgatcga ttactcgcga
     6721 cgttgcgccg gctcgcccag ttgggcaaca cggtcgtcgt tgtcgaacac gatgaagaca
     6781 ccatgcgcgc ggccgatcac ctcgtcgact ttgggccagg gccaggcgtc aagggaggcg
     6841 aagtcgtcgc gcaggggacg atcgatgatc tggcacgcac acgtgagagc ctgaccggtg
     6901 cctaccttgc cggcaagagg gcgatcgcga ttccggcgga acgcaaaatt cccgacggcc
     6961 gatttctcac gatcaaggga gccacgcata ataatctcaa agacatcgac gcggcattcc
     7021 cgctagggct ctttatctgt gtaacgggtg tctccggctc gggcaaaagc tcacttgtgg
     7081 gagatatcct ccgcgaggcc ctggcgcgcg agctgaaccg ggctgtcacc gatccgggcg
     7141 cgcacgcggg catcgagggg ctcgagcatc tggacaaggt aatcgacatt gaccagtcac
     7201 cgatcggccg aacaccacgt tccaacccgg caacatatat caagcttttt gatctgattc
     7261 gcgatctctt cactcggcta cccgaggcaa aagcacgcgg ttatgcgccg ggccgattca
     7321 gctttaacgt ctcgggaggc cggtgcgagg catgcgaggg aaacgggtcg aatcgactgg
     7381 aaatggactc ccttgccgat gtctgggtca cctgcccggt ctgccagggc aaacggtttg
     7441 gccgcgagac acttcaggtt cggttcaagg gtaagagcat cagcgacgtg ctcgacatgg
     7501 acgtgcaaga ggcactcgaa cattttgcca acattcccaa gatcgccgtc atgctccaga
     7561 ccttgcacga cgtgggtctc ga
//
LOCUS       DATAQL010000744         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_228831, whole genome shotgun sequence.
ACCESSION   DATAQL010000744 DATAQL010000000
VERSION     DATAQL010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228831"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..2576
                     /locus_tag="VHS97_12905"
     CDS             84..2576
                     /locus_tag="VHS97_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3449150.1"
                     /translation="MSVWWAGAGLALASVASPPTVAIAAAGSRVDYASQVKPILTRHC
                     VSCHGATKPKGGLRLDTAAAALLGGESGPVVLPGRGEQSPIIAAVRGDGEFERMPLHR
                     PPLADAEIKLLSDWIDQGAKAIAGERPGIPSHWAFIPPKRPVVPEVRKPDPARNPIDR
                     FILARLERAGLAPAPEADRATLLRRLSLDLIGLPPAPEEVDAFLASPPADADERTVFR
                     LLASPHFGERWARPWLDLARYADSNGYSIDAPRSIWKYRDWVIAALNADMPFDEFAID
                     QLAGDLRPGNPRGPSVASGFHRNTPINQEGGIDVEQFRVEAVVDRVNTTGTAFLGLTI
                     GCCQCHDHKYDPISQREYYRLFAFFNNVDEPDLEIAAPADLARRQQVRDRIDEFHSAL
                     ATRYPDLDDHERKWEGTLTLEFTQAQDPLVRVAFDLPREKRTPTQRRALVELMMARDP
                     TFRVEFAELMKLRAAEPKFVTTMVVRERSGSPRPTHVHLGGDFTRKGERIEPGVPGVL
                     PPLEDSSAGNPADRMDLARWLVDRRNPLAARVVVNRIWQAYFGRGLVETDDDFGTQGS
                     APSHPELLDWLACELMDRGWSRKAIHRLIVSSATYRQASRLRPEGQAIDPDNRLLWRQ
                     ARLRLDAELIRDAALTTSGLLTRVLGGPSVFPPQPEGVMTLGQMKRPWKADSGPNRYR
                     RGLYTFFWRATPHPFLTTFDAPLGVQTCTRRVRSNTPLQALTMLNDPALVEIAEGLAA
                     RILSERPDPASDRDRIRQGIMLCLGRPALDRELDMLESVLAQERADGVVPETQAAGQA
                     AEPTRHGGLSPSAPWITVARVLLNLDEFVTRE"
     gene            2614..>3277
                     /locus_tag="VHS97_12910"
     CDS             2614..>3277
                     /locus_tag="VHS97_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3449151.1"
                     /translation="MSGDSDSRRLIDHVLCRRTRRHFFADCGVGLGAMALASLSQGQA
                     AKGAMAEPDGNPPRPLEPGEATARGANPLAARPGHLAARAKSVIYLFMAGGPSQLELF
                     DYKPQLQKYSGQPIPDSFIQGRRFAFMDLFTKEHPKLLGTVRRFARHGQSGAWVSALL
                     PRLAEVVDDLTIVKSVATDVFNHAPAKLFANTGTVQFGRPSMGSWVTYGIGSESADLP
                     GFV"
BASE COUNT          530 a         1080 c         1091 g          576 t
ORIGIN      
        1 ggtgtcgcac cgggtggttg tctcgccgat ttcctacctg aacatcgccg acgatttctc
       61 aaatgggcgc tgtgcacctt cgaatgagtg tttggtgggc cggggccggt ttggcgttgg
      121 cctcggtcgc ctccccgccc acggtcgcca tcgcggcggc gggttcgcgg gtcgattacg
      181 ccagtcaggt caagccgatc ctgacccgtc attgcgtgtc gtgtcacgga gcgaccaaac
      241 cgaagggcgg gctgcggctc gacactgctg cggcggcgct tctggggggt gagagcgggc
      301 cagtcgtact cccgggccga ggcgagcaga gcccgattat tgccgcagtg cgaggcgacg
      361 gggagttcga gcgcatgcct ctgcatcgtc cgccgctggc cgatgccgag atcaagctgc
      421 tcagtgactg gatcgaccag ggggccaagg cgatcgccgg cgagcggccc ggaattccgt
      481 cgcactgggc gttcattcca ccgaagaggc cggtcgtccc ggaggttaga aagcccgacc
      541 cggcgcggaa tccgatcgac cgcttcatcc tggctcggct cgaacgggct gggctggctc
      601 ccgcgcccga agccgaccgg gcgactttac tgcgccgcct gagcctcgac ctgattggtt
      661 tgccgcccgc gccggaagag gtagatgcct tcctggccag cccgccggct gatgctgatg
      721 aacgcacagt ctttcggctg ctggcctcgc ctcactttgg cgagcggtgg gcccgcccct
      781 ggctcgactt ggctcgctat gcagactcca acggctacag catcgacgcc ccccgctcga
      841 tctggaagta ccgtgactgg gtcatcgccg cactcaacgc agacatgccg ttcgacgagt
      901 tcgcgatcga ccagctcgcc ggcgacctcc ggcccggaaa tccgcgcgga ccgagcgtcg
      961 ccagcggttt tcaccgcaat acgccaatca accaggaagg ggggattgac gtcgaacagt
     1021 ttcgcgtcga ggcggtcgtc gaccgggtca acaccacggg cacagccttc ctgggcctga
     1081 cgatcggctg ctgccagtgc cacgaccaca agtatgatcc gatctcccag cgtgagtatt
     1141 accgcctctt cgccttcttc aacaatgtcg atgagccgga cctcgagatc gccgcgccgg
     1201 cagatctggc caggcggcag caggtccgcg accggatcga cgagttccac agtgcgctgg
     1261 cgacacggta tccagacctg gatgaccacg agcgaaagtg ggagggaacg ttgacgctcg
     1321 agttcaccca ggcccaggac cctctagtcc gagtggcatt cgatttgccg cgggagaagc
     1381 gaactccaac ccagcgccgc gcgttggtcg agctgatgat ggcgcgcgac ccgacttttc
     1441 gggtcgagtt cgcggaactg atgaagctgc gcgccgccga gcccaagttc gtgacgacga
     1501 tggtcgtgcg ggagcgatcg ggcagcccgc gtccgacgca tgtgcatctt ggcggcgact
     1561 tcacgcgcaa aggagagcgg atcgagccgg gcgttcccgg cgttctgcct cccttggagg
     1621 attcgagcgc gggcaatccg gccgatcgaa tggaccttgc tcgctggctg gtcgatcgcc
     1681 gcaacccact ggcggcccgc gtggtggtca accgcatttg gcaagcctat ttcggccgcg
     1741 gcctggtcga gaccgacgac gactttggta cccaaggttc agcccccagt catcccgagt
     1801 tgctcgactg gctggcctgc gagctgatgg accgtggttg gagccggaag gcgatccacc
     1861 ggctgatcgt gagctcagcg acctaccggc aggcgtcgcg attgcgtccg gaggggcaag
     1921 cgatcgatcc cgacaaccgt ctgctgtggc ggcaagcccg cctccgcttg gacgccgagc
     1981 tgatccgcga cgccgccttg acgactagcg gtctcctgac gcgtgtgctc gggggcccca
     2041 gcgtctttcc tccccagccc gagggtgtga tgaccctggg tcagatgaag cgcccgtgga
     2101 aggccgattc cggcccgaat cgctatcgcc gtggcctgta tacctttttc tggcgggcca
     2161 cgccccaccc gttcttgacg acattcgatg cgcccttggg cgtgcaaacc tgcacgcgcc
     2221 gggtccgatc caacacgccg ttgcaggcgc tgacgatgct caatgacccg gcgctcgtcg
     2281 agatcgccga agggctggcc gcgcggatcc tgagcgagcg gccggaccca gcctcggacc
     2341 gcgaccggat ccggcaggga attatgctat gcctgggccg cccggcgctc gaccgcgagc
     2401 tggacatgct ggagtcagtg ctggcgcagg agcgcgctga tggtgtggtt ccggaaacgc
     2461 aagccgctgg tcaggcggcc gagcctacgc gccacggtgg tctctcgcct tccgctccct
     2521 ggatcaccgt ggcccgcgtg ttgctcaacc tcgacgagtt cgtgacccgc gagtaaacct
     2581 gttcgcaacg gcaagcgggg agggctgacg gccatgtccg gcgactccga ttcacgacga
     2641 ctgattgatc acgtcctctg ccgccggacg cggcgccatt tcttcgccga ttgcggcgtg
     2701 gggctggggg cgatggcgct ggcctcactc tcgcaaggcc aggcggcaaa gggcgcgatg
     2761 gcagagcctg atgggaatcc gccgcgcccg ctcgagcccg gcgaggcgac ggcacggggc
     2821 gccaatcctc tggccgcccg gccgggccat ctggccgcgc gcgccaagag cgtcatctat
     2881 cttttcatgg ccggcggtcc gagtcagctc gagcttttcg actacaagcc gcagctccag
     2941 aaatacagcg gccagccgat cccagactcg ttcatccagg ggcgccggtt cgcgttcatg
     3001 gatctattca ccaaagagca tcccaagctg ctgggaacgg tccgccggtt tgccaggcac
     3061 ggacaatcgg gggcatgggt gtcggcgctc ttgcctcgcc tggccgaggt ggtcgacgac
     3121 ctgacgatcg tcaaatcggt ggcgaccgat gttttcaacc atgcgccggc aaagctattt
     3181 gccaatacgg ggacggtgca gttcggccgg ccgagcatgg gttcgtgggt tacgtacggc
     3241 atcggcagcg agtcggccga cctgcccggc ttcgtgg
//
LOCUS       DATAQL010000745         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5420444, whole genome shotgun sequence.
ACCESSION   DATAQL010000745 DATAQL010000000
VERSION     DATAQL010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5420444"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VHS97_12915"
     CDS             <1..496
                     /locus_tag="VHS97_12915"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX3449152.1"
                     /translation="GTVSFLLGSTMLATVPLGNDGTATFSTASLPLGTYGITAVYNGA
                     GVILSSRSAAVTESVVPYKQTVNPMTTTTALSIATQSLPHGRTEYVLVATVAPAGGNP
                     PTSPAGIVVFRRNGRTLGKAKIAGGVARIVLGRRRPSSKLTFLAAYQGNSQFRASTSL
                     PYRA"
     gene            525..1109
                     /gene="rsmD"
                     /locus_tag="VHS97_12920"
     CDS             525..1109
                     /gene="rsmD"
                     /locus_tag="VHS97_12920"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX3449153.1"
                     /translation="MRVVGGRARGKPLRLVPGKGTRPILDRVKTALFDILRPRIEGMR
                     VLDLFAGSGSVGIEALSQGAAHCTFIDLGHQAIATIKQNLLTTGFSESCAVHQTNALD
                     FLRSTPRSFDLIYIAPPQYKHLWVEAMRVLAARPDLLAAASEDNTGPGLAIAQIDPKE
                     YESLDLGALRETRQKRYGNTLLVFFERPGVGTAD"
     gene            complement(1132..2280)
                     /gene="cax"
                     /locus_tag="VHS97_12925"
     CDS             complement(1132..2280)
                     /gene="cax"
                     /locus_tag="VHS97_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX3449154.1"
                     /translation="MKVTHLGFAAVGELRLLKMNWLLVFVPIAIALDWWGASPILVFA
                     ASAVGIVPLAGLMGDATEALAEYLGPTLGGLLNATLGNAPEIIISLFALKRGLIGIVK
                     SSLTGSIIGNLLFGLGVSMFAGGLKVKTQKFDSRVARINGALLVLAAFGLIIPAIYRM
                     SPAAGEDTSRHVSLEIAVILFVVYLGSLVFTLTQKAVMGTAGVKAEKIEAGEPVADVE
                     EVEVGWSRNKALAILAGVTIVLAVMSEVLTGAIDPAAETMGLTPLFAGVFLLALVGNA
                     AELLNAVRFARKDKMDLAIGVTVGASIQVALVVAPILVFAGYFMGQDMNLHFTRFEMW
                     AVVLAVFITRTLIYDGESTWLEGLMLVAIYLMLGFGIFHLPPEAANAF"
     gene            2696..>3222
                     /locus_tag="VHS97_12930"
     CDS             2696..>3222
                     /locus_tag="VHS97_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449155.1"
                     /translation="MNVMNRLGVAFGLLFLLAFGRTVPAQTSGTTPPPPPPPGPVASP
                     PLEGPVTDLEPIRPDDTTGRVSGRTEQDPAPAGASHDALQDKSARPNSERVYTPKQPP
                     RPIAERPSGPRPGRRATWVPGYWEWDPGRAEFVWIGGVWQMPPLGSIWVTPRWMRDRD
                     GWYRTSGFWSRRRDNG"
BASE COUNT          680 a         1044 c          937 g          561 t
ORIGIN      
        1 gggtacggtc agtttcttgc taggctcaac gatgctggca accgttccct tgggcaacga
       61 cggtacggcg acgttttcga ccgcgtcgct accgctcggg acatatggaa tcacggccgt
      121 ttataacgga gcaggggtga tcctgagctc tcgaagtgcg gcagttacgg agtcggtggt
      181 accttacaag caaaccgtca acccaatgac gacgacgacc gcgctgagta ttgcgaccca
      241 atccctaccc cacggccgga ccgagtacgt cctggtggcc acggtcgcgc ccgccggagg
      301 aaacccgccg acatcgcccg cgggcatcgt cgtctttcgc agaaacggcc ggactctcgg
      361 taaggccaag atcgccgggg gcgtcgcccg gatcgtcctg ggccgcaggc gtcctagcag
      421 caagttgaca ttcctcgcgg cttaccaagg gaattcgcag ttccgggcga gcacatccct
      481 cccgtacagg gcttaggtct gagcattcga caaaggggcg acacatgcgg gtggtgggtg
      541 gtagggcgcg cggcaagccg ttacgcctgg ttccgggaaa agggacgcgc ccgatcctcg
      601 accgcgtcaa gacggcgctt ttcgacattc tccgcccgcg tatcgagggc atgcgagtgc
      661 tcgatctctt cgctggcagc ggcagcgtcg gcatcgaggc cctcagccag ggagcggccc
      721 attgcacttt catcgacctc ggccaccagg ccatcgccac catcaagcag aatctactca
      781 cgaccggctt ttccgaatct tgcgccgtcc atcagaccaa cgcgcttgat ttcctccgct
      841 cgactccgcg gtcattcgac ctgatctaca tcgccccacc tcagtacaaa cacctctggg
      901 tggaagcgat gcgcgtgctg gccgcgcggc cagacctgct cgctgcggcc agtgaagaca
      961 ataccggccc tggactcgcg atcgcccaga tcgatcccaa ggaatatgaa agtctggatc
     1021 tgggcgcgct gcgcgaaaca cggcaaaagc ggtacggcaa caccctcctg gtgttcttcg
     1081 agcgtccggg cgtcggcacc gcggactagc tgccgtgcac tggcgctctc ttcaaaacgc
     1141 gttggcggcc tcgggcggca ggtggaaaat gccgaacccg agcatgaggt agatggcgac
     1201 cagcatcaga ccctccagcc aggttgattc gccgtcgtag atcaacgtcc gtgtgatgaa
     1261 cacagccaga acgaccgccc acatctcgaa ccgcgtgaag tggagattca tatcttggcc
     1321 catgaagtat ccggcaaaca ccaggatggg cgcgacgaca agcgccacct ggatgctggc
     1381 ccctaccgtc acgccgatcg ccaaatccat cttgtccttg cgggcaaagc ggaccgcgtt
     1441 taacagttcg gcggcgtttc ccacgagcgc cagcaggaaa acgccggcaa acagcggcgt
     1501 cagccccatc gtctcggcag ccggatcgat cgcgcccgtc aagacttcac tcatgaccgc
     1561 caacacgatc gtcacacccg ccaggatcgc gagcgccttg tttcggctcc atccgacttc
     1621 cacctcttcg acgtccgcca ccggctcgcc cgcctcgatc ttttcggctt tcacaccggc
     1681 tgttcccatc acggctttct gggtcagcgt gaaaaccaga ctccccaggt agaccacgaa
     1741 cagtatcacc gcgatctcga gcgaaacgtg ccggctggtg tcttcaccgg cggccgggct
     1801 catccggtag atcgccggga tgatcaaccc aaacgcagcc agtaccaaga gtgcaccgtt
     1861 gatccgggcc acgcgtgagt caaacttctg agtcttgacc ttgagaccac ctgcgaacat
     1921 cgacacgccc agaccaaaca agagattgcc gatgattgaa ccggtgagcg acgacttgac
     1981 gattccgatc aacccccgct tgagtgcgaa caggctgata atgatctcag gcgcgttgcc
     2041 caaggtcgcg tttagaagcc ctcccaacgt cggtccgaga tactcggcca aagcctcggt
     2101 cgcgtctccc atcaaaccgg caagaggcac gatccccacg gccgaggcag caaacaccag
     2161 gatcggactg gcaccccacc aatcgagagc gattgcgatc ggaacgaata ccaaaagcca
     2221 attcattttc aagagacgaa gctcgccgac agccgcgaat ccgaggtgcg taactttcac
     2281 cccggcactc gttcaccggg tcacagcata gccacgagcg atcgcagcgt cagcgcgccg
     2341 ccgagacggg ccaagggcgc gggctaaccg cgaagaacgc aaagaacgcc aagaaagtgc
     2401 gaataatgcc tgagagcgag acggatgatt tcgtgcgagt gacgatgatt gggcgaaggc
     2461 gaaacattac ggttgtcgac cttggcgcgg cattgagaca ccgcgctggt gtagtgaaac
     2521 ttagcggccc tcgttgaatc gctactatag aaaccagatg aagactccgt tgcgcgggat
     2581 tgcattccga gcagcaaacc agtggctgat cgtggcacac tttttgcggt cacggaaatt
     2641 aggtaaactt gatctgtgag caccaacgag gttcatgccc gccggagaac ctgagatgaa
     2701 cgtgatgaac cgactcggag tcgcttttgg acttcttttt ttactagcgt ttggccgaac
     2761 tgtgccagcc cagacctcgg gaacgactcc gccgccgccg ccgccaccgg gccccgtggc
     2821 ctcgccaccg cttgaaggtc cggtgaccga ccttgagccc attcgaccgg acgacacgac
     2881 cggacgagtt tccgggcgta ccgagcaaga tccggcacct gccggcgcga gccacgacgc
     2941 gctccaagac aaaagcgctc ggcccaactc agaacgtgtg tatactccca agcaaccgcc
     3001 ccggccgatc gccgagcgcc cgtcaggtcc acgtccaggt cgccgtgcca cctgggtgcc
     3061 tggctactgg gagtgggatc cagggcgggc ggaatttgtc tggatcggcg gcgtgtggca
     3121 aatgccaccg ttaggctcga tctgggtcac cccccgctgg atgcgcgatc gagacggctg
     3181 gtatcgcacc tcggggttct ggagccgccg ccgcgataac gg
//
LOCUS       DATAQL010000746         8378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4809128, whole genome shotgun sequence.
ACCESSION   DATAQL010000746 DATAQL010000000
VERSION     DATAQL010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4809128"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VHS97_12935"
     CDS             <1..1592
                     /locus_tag="VHS97_12935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449156.1"
                     /translation="PAIATGPKPHPITDISVVRARLRELPMIHILILAMANCWRYMIL
                     GDNDPVLRYLDLSILALLAAAIALLWSRWRFSLAWLQALELAMIGILAMRLAIIEYRQ
                     MLRFSLWHEPAMAQFIMKNIVLLDAVLIASYGIYVPKNWRHAALVVGPLALLPFATLS
                     VLYLQHPDAMAWLGQGAIGSSSPRYLLHSFDAMVLLILAVGSTFGARMISLLRRQVAE
                     AKHLGQYRLRRQIGAGGMGEVYLAEHQLLKRPCALKLLRPESVADPRTLARFEREVRL
                     TAALSHPNTVEIYDYGRTDKGTYYYVMEYLPGQSLAELVERHGPQPPGRVVYLLRQIC
                     QALREAHAAGLIHRDIKPSNIFVSQRGGMDDVAKLLDFGLVLPIAKTGAPQLSREDQI
                     LGTPLFMSPEQATGGGELDGRSDIYSLGAVAYFLLTGRPPFEDEDGIRLMIAHARDPV
                     VPPSLLYVDVPRDVERVVLRCLSKEPADRFPNSESLEKALGNCACVVDWDQDRAAQWW
                     KAADRARGRVRGPQNPTSAGS"
     gene            1917..2243
                     /locus_tag="VHS97_12940"
     CDS             1917..2243
                     /locus_tag="VHS97_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3449157.1"
                     /translation="MQQEDNAAVPTNPVMSGWLEEALASSGYTPPRVVSGAGHDAGVM
                     AAVTPMAMLFLRSPGGVSHHPDERVLPEDVAVGLEVLIHYLQLLAGRIGTIGAISRPV
                     HTVGVA"
     gene            2244..2996
                     /gene="allE"
                     /locus_tag="VHS97_12945"
     CDS             2244..2996
                     /gene="allE"
                     /locus_tag="VHS97_12945"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HEX3449158.1"
                     /translation="MELFGTTRSRLRRSHALIAPDSFVTSDPPGWERSQVINLISPRM
                     GARFSQYLALMESGGSAGQPPRGVERVIYVVEGGVDLGYKGSHVRSLGPGGFAYSPPD
                     ALVEVRASETSSINVFEKRYVNRRGFSIPEPLFGDDGEVEGTAFMGDPDAILKVLLPR
                     DPRFDLAVNVFTFQPGTSLPLVEVHVMEHGLLMLAGKGVYRLGDDWYPVRQGDVIWIA
                     PYCPQWFVAMGKNPARYLYYKDVNRDPLEDEA"
     gene            2993..4243
                     /locus_tag="VHS97_12950"
     CDS             2993..4243
                     /locus_tag="VHS97_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEX3449159.1"
                     /translation="MNIARLGVDACEIMAELDGLAARSDTPLPAVTRVVYTAPDLAAR
                     AFIRDLCNDAGLAVREDAIGNTFARWEGQKAGLAPVGTGSHIDAIPHSGRFDGTVGVI
                     GGLAAIRSLKRAGFQPIRPVELLIFTSEEPTRFGIGCLGSRALAGSMTPEALRALKDV
                     DGRSFDDIRQEAGFHGALANVRLPAGYFAAFVELHIEQGPLLERAGVPIGIVTAIAAP
                     AALRVTCKGEGGHAGTVLMNGRRDALCAAAEVVLAVEASARSTESPDTVATTGVCRVH
                     PGAINSIPEHVTLEIDIRDVDLTRRDRVLETVGSAIKAIAARRQVAAVTQCLSADPPA
                     STAPFVVQAIQAACRTLGLENVPMVSRAYHDSLFMAAIAPTGMIFVPCKEGISHRPEE
                     YSEPEAIARGVEVLALALKELAEK"
     gene            complement(4268..5113)
                     /gene="pucL"
                     /locus_tag="VHS97_12955"
     CDS             complement(4268..5113)
                     /gene="pucL"
                     /locus_tag="VHS97_12955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEX3449160.1"
                     /translation="MPAILAQHSYGKSRIRVTKVTRLADRHEVRELTIEIELEGDFAR
                     SYTGGDNSLIIPTDTMKNVGYALAKEHPLESIEDFGSAIANHFLKHHSHVERATIRIV
                     EHPLERIEVDGRGHPHAFCGLRRETRTSTVRGWRGGARVESGIDELFLLKSTGSAFTG
                     FLRDRYTTLSDASDRILATILKAEWLYAADPPDWVLARMKIRQTLLETFAGHQSLSAQ
                     QTLYAMGAAALEACLQIDRITLTMPNKHRILVDLQPFGLENANEVFLATDEPHGTITG
                     TLQRA"
     gene            5245..6612
                     /gene="allB"
                     /locus_tag="VHS97_12960"
     CDS             5245..6612
                     /gene="allB"
                     /locus_tag="VHS97_12960"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX3449161.1"
                     /translation="MGAWQSAGRHGGGHARGHGRDRRRKNRRRDRFPESTRFIAGRGC
                     GGSVVLPGLVDTHVHINEPGRTEWEGFTTATRAAAAGGITTLVDMPLNCSPVTTTALA
                     LALKIQAARDKLWVDCGFYGGVVPGVSGQIGPLIAAGVLGFKAFLCHSGIDEFPNATE
                     SDLRTVMPELARAGVPLLVHAELTGPAPVSAPTTAILNRSYNRHLASRPREWEHEAIR
                     LLIDLCRDYGCHVHIVHLSSADSIPLLARARAEGLPLTVETCPHYLSFAAEEIPDGDP
                     RYKCAPPIRERENRERLWEGLQQGMIDTIGSDHSPAPPELKHLETGDVWRAWGGIASL
                     QLSLPIVWTEARRRGFPIDELARWMARDPARLVGISDMKGEIAPGRDADLVVFDPDAT
                     FTVEGTKLYHRHKATPYEGQVLAGRVEATYLRGRAVYRSGDLVETPWGQTLLAVNSRS
                     TGAIS"
     gene            6609..7115
                     /gene="uraD"
                     /locus_tag="VHS97_12965"
     CDS             6609..7115
                     /gene="uraD"
                     /locus_tag="VHS97_12965"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX3449162.1"
                     /translation="MNLELINAWTDDEARESFQRCCGSRRWFEAMARARPFESEAALI
                     ETAERIWWALSTADWLEAFGAHPRIGDVDAIRARFAATAAWASREQAGALGASEEVLQ
                     NLARGNRQYEDRFGYIFIVCASGKTAEEMLGLLMQRLGNDADAEIKLAAGEQMKITRI
                     RLERIATS"
     gene            7112..7456
                     /gene="uraH"
                     /locus_tag="VHS97_12970"
     CDS             7112..7456
                     /gene="uraH"
                     /locus_tag="VHS97_12970"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX3449163.1"
                     /translation="MSPITTHVLDTTLGKPGRGIAVVVEIGLDDGSWTELACGVTDGD
                     GRIAQFSPPLKAPKPGLYRVRFFTAAYFTAMQVRGFYPEVDVIVQIDEPDQHYHIPLL
                     LSPFGYSTYRGS"
     gene            complement(7520..7996)
                     /locus_tag="VHS97_12975"
     CDS             complement(7520..7996)
                     /locus_tag="VHS97_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3449164.1"
                     /translation="MPQSLAINLIHLIYSTKDRAPGLSPKIRDGLFAYKSGILKEWRS
                     PSLLIGGVADHVHVLFCLSKNHALAKVIEELKKGSSKWLKTQGPEFRGFHWQAGYGAF
                     SVSQSHVERVRLYIQRQEEHHRTHSFQDELLAFLNRYKMEHDERFPLGIITTGQII"
BASE COUNT         1546 a         2534 c         2632 g         1666 t
ORIGIN      
        1 ggccagcgat cgccaccggc ccgaagccgc acccgatcac cgacatatcg gtggtgcggg
       61 cgcggctgcg cgaattgccg atgatccaca tcctcatctt ggcgatggcc aactgctgga
      121 ggtacatgat ccttggggac aacgaccctg ttcttcgtta tctcgatcta tccatcctcg
      181 cgctccttgc ggccgcgatc gccttactct ggagtcgttg gcgcttctcg ctcgcctggc
      241 tccaggctct ggagctggca atgatcggca ttttggctat gcggctcgcg atcatcgagt
      301 atcgccagat gctgagattc tcactatggc acgaaccggc gatggcccag tttattatga
      361 agaacatcgt actcctcgac gccgtcctga tcgccagcta tggtatctac gtccccaaaa
      421 attggcgaca tgccgcgctt gtcgtgggac cgctcgcgct cttacccttc gcaacattgt
      481 cggttctcta ccttcagcat cccgacgcaa tggcctggct ggggcaagga gcgatcggaa
      541 gctcgagccc acggtacttg ttgcacagct tcgatgcgat ggtcctgctc atcctggctg
      601 tcggctccac attcggagcc cgcatgatct ctctgttacg gcggcaggtc gccgaggcca
      661 agcatctcgg ccaatatcgc ctgcggcggc agatcggcgc cggcggcatg ggcgaggtct
      721 acctggctga gcaccagctg ttgaagcgcc catgcgcctt gaagctgctt cggccggaaa
      781 gcgtggccga tccaaggacc ctggcccggt tcgagcgcga ggtccgcttg accgccgcgc
      841 tctcgcaccc caacacggtc gaaatctacg actacggccg caccgacaag ggaacctact
      901 actatgttat ggaatacctg ccgggtcaga gcctggccga attggtcgag cgacacggcc
      961 ctcagccgcc agggcgggtg gtgtacctct tgcgccagat ctgccaggcc ctacgcgagg
     1021 cgcacgcggc gggcctgatc catcgcgaca tcaagccgtc gaacatcttc gtctcgcaac
     1081 ggggtgggat ggatgatgtc gccaaactgc tcgatttcgg gctggtcctg cctattgcca
     1141 agaccggtgc gccccaactg agccgagaag atcagatcct cggtacgccg cttttcatgt
     1201 cgcctgagca ggcaaccggc ggcggcgagc tggacgggcg gagcgacatt tactcgctcg
     1261 gcgccgtcgc ctacttcctc ctgaccggac gtccgccgtt cgaagacgag gacggaattc
     1321 gcctcatgat cgcgcatgca cgagacccgg tcgtgccccc gtccctgctc tacgtcgacg
     1381 tcccacgtga tgtcgagcgc gtcgtcttac gttgcctgtc caaggagccg gccgatcgtt
     1441 tcccgaattc ggagagcctg gaaaaagcgt tgggcaattg tgcttgtgtg gtggactggg
     1501 accaggatcg cgccgctcaa tggtggaaag ccgcagaccg cgctcggggg cgcgtgcgag
     1561 gcccacaaaa cccgacctct gccggttcgt gatcgagatc gatggcggta ctgcaccatt
     1621 gcggctttgc cgagaacctc gcgggaactg ttctgcagac gttttccgac ctttccggct
     1681 cctttcgtga aagctagact cggagagctc gtctccccct tcgcagggaa attcccgatg
     1741 catggacaat ctaacggatt cgaagcccga aacgacctgg cggccgaagt gctggtgcgg
     1801 tgcgacgaag tcgccctgtt ctcggaggta ccggacaata tcatacgcac ctttctcggc
     1861 gagcccatgc gccgccttca cggacgactt actcaatgga tgtggagttc ctcgtcatgc
     1921 aacaagaaga caacgctgca gtccccacca atccggtcat gagcggttgg ctggaggaag
     1981 cgcttgcctc cagcggctat acacccccgc gcgttgtcag cggtgccggc cacgacgccg
     2041 gcgtgatggc tgcggtgaca ccgatggcca tgctctttct ccgtagtccg ggcggcgtga
     2101 gccatcaccc cgacgaacgc gtgctgcccg aggacgtcgc ggttggtctc gaagtcttga
     2161 tacactatct ccagctcttg gctggccgca tcggaaccat cggtgcgata tcccgacccg
     2221 tccacaccgt gggagtcgcg tgaatggagc tcttcggcac gacccgtagc cgcctccggc
     2281 ggtcgcacgc cttgattgcg cccgacagct ttgtcacctc ggaccccccg ggctgggagc
     2341 ggtcgcaagt tattaacctg atctcaccgc ggatgggagc gcggttctca cagtacctcg
     2401 cgctcatgga atccggcgga tccgcgggcc agcctcctcg gggcgttgag cgagtgattt
     2461 acgtggtaga aggaggggtc gatctcggtt acaagggttc gcatgtgcga tccctcggcc
     2521 ctggcggatt cgcctactcc cctcccgatg cgctcgtcga ggtacgggcg agtgaaactt
     2581 cgagcatcaa cgtatttgaa aagcgttacg tgaatcgccg cggtttttcg attccggagc
     2641 ccctttttgg agatgatggt gaagttgaag gcaccgcctt catgggtgac ccggacgcca
     2701 tcctcaaggt actattgccg cgcgacccgc ggtttgacct ggccgtcaac gtcttcacgt
     2761 ttcagcccgg gacgtcgctc ccattggtcg aggtccatgt aatggaacat gggctcttga
     2821 tgttggccgg aaagggcgta tatcgactgg gtgacgactg gtatccggtg cggcaggggg
     2881 acgtcatctg gatcgcgcct tactgcccgc agtggtttgt ggcgatgggc aaaaaccccg
     2941 cgcgctacct ttattacaag gacgtgaacc gcgacccgct ggaggacgag gcatgaacat
     3001 tgcgcgcctc ggtgtggacg cgtgtgagat catggccgag ctcgacggcc tggcggccag
     3061 gagcgacacc cctctacctg cggtgacccg ggttgtctac accgcgcccg acctggcggc
     3121 gcgtgctttc atcagagacc tttgcaacga tgccgggttg gcggtccgcg aagatgcgat
     3181 cggcaacacc ttcgcgcggt gggaggggca gaaggccggg ctagcccctg tcgggacagg
     3241 ctcgcatatc gacgcaatcc cgcactccgg ccgattcgac ggcactgtcg gtgtcatcgg
     3301 cggtctggcg gcgatccgct cgctcaagcg cgccggcttt caacccattc ggccggtcga
     3361 gctcttgatc ttcactagcg aagagccgac gcgattcggc atcggctgcc tggggagcag
     3421 ggcgctcgcc ggctcgatga cgccggaggc cctgcgagcg ctcaaggacg tcgacggccg
     3481 ttcgtttgac gatatccgcc aagaggcggg tttccacggt gcgctcgcca atgtgcggct
     3541 cccggccggg tattttgccg ccttcgtcga gctgcacatc gaacaaggtc cgctgctcga
     3601 acgggctgga gtgccgatcg gcatcgtcac cgcgatcgcc gcgccggcgg cgctacgcgt
     3661 cacatgtaaa ggggaaggtg gacacgcggg gaccgtcttg atgaacggcc ggcgcgatgc
     3721 cctatgcgcc gccgccgaag tggtgctcgc cgtcgaggcg agcgcccggt caacagagag
     3781 tcccgatacg gtcgcgacca cgggcgtttg ccgcgttcac cccggcgcga tcaacagcat
     3841 ccccgagcac gtgacactcg agatcgacat tcgcgacgtg gatttgaccc ggcgcgatcg
     3901 tgtgctggaa accgtcggca gcgcaatcaa ggcgatcgcc gcacgcaggc aggttgcggc
     3961 ggtgacgcaa tgcctgagcg ctgacccacc tgccagcaca gcgccatttg tcgtccaggc
     4021 gatccaggcc gcttgccgca cactggggct cgagaacgtg ccgatggtaa gtcgagccta
     4081 tcacgattcg ctcttcatgg ccgcgatcgc gccaacgggg atgatctttg tcccgtgcaa
     4141 ggaagggatc agccaccgac ccgaagagta cagcgaaccc gaagcgatcg cccgtggagt
     4201 cgaggtgctg gctctcgcac tcaaagaact ggcggagaaa taaagactca gttgcgatat
     4261 agaacgatca tgcacgttga agtgttccgg tgatcgtgcc gtgaggttcg tcggtggcga
     4321 ggaaaacttc gttggcattt tccagaccaa acggctgcag atcgacgaga atacgatgct
     4381 tgttgggcat ggtgagcgtg atgcggtcga tttgaaggca cgcctcgagc gccgcggctc
     4441 ccatggcgta gagagtctgc tgcgcggaga ggctctggtg gcccgcgaag gtctcgagca
     4501 acgtctggcg aattttcatg cgcgcaagga cccagtcggg cggatcggcc gcgtacagcc
     4561 attcggcttt gagtatcgtg gccagaatgc ggtcggaagc atcggacagc gtggtgtacc
     4621 ggtcgcggag gaagccggtg aaggccgaac cggtcgactt caggaggaac agctcgtcga
     4681 tgcccgactc gacgcgcgcg cctccgcgcc agcctcggac ggtgctcgtt cgcgtttcgc
     4741 ggcgcaggcc gcagaaggcg tgggggtgcc cgcggccatc gacttcgatg cgctcaaggg
     4801 gatgctcaac gatgcggatt gtcgcccgtt caacgtgact atgatgcttg agaaagtggt
     4861 tagcgatcgc gctcccaaaa tcctcgatcg attccagggg gtgttccttg gccagcgcgt
     4921 atcccacgtt tttcatcgtg tccgtgggta tgatcaaact gttgtctccg ccggtgtacg
     4981 aacgcgcgaa atccccttca agctcgatct cgatcgtcaa ctcgcggact tcgtggcggt
     5041 cggccagacg cgtgaccttg gtgacacgaa tgcgcgactt gccgtaggaa tgttgcgcaa
     5101 ggattgcagg cacgatcgca tacctcaaat ggcccggcaa tgtcgccgtc attaccaatg
     5161 atcataatgt cgccccgtcg gggagtgcag tgggatgctc gaaaagttgc caggcggaac
     5221 tgaggctcta gaagatcacg gcacgtgggc gcttggcagt cggcgggtcg tcacggcggg
     5281 gggcatgcgc gcggccatgg tcgtgatcgc cggcgaaaaa atcgtcgacg tgaccgattt
     5341 ccggagtcta cccgattcat tgccggtcga ggatgcgggg gatcggtcgt cttgcccggg
     5401 ctcgttgaca cacacgtgca catcaacgag cccggacgca cggagtggga gggattcacg
     5461 accgcgacac gtgcggccgc cgcgggcgga atcaccacac tggtcgacat gccactcaat
     5521 tgttcgcccg taacgacgac ggcccttgcc ctggcgctca agatccaggc ggctcgcgat
     5581 aagctctggg ttgactgcgg attctatggc ggagtggtgc ctggcgtgag cggacagatc
     5641 gggccattga tcgcagccgg cgtgctcggt ttcaaggcct tcctgtgcca ttcggggatc
     5701 gacgagtttc ccaatgcgac cgagtccgat ctccgcacgg tgatgccgga actagcacgc
     5761 gccggcgtgc ccctgttggt gcacgccgag ctgactggtc cagcgcccgt gtcggcacca
     5821 acgactgcca tcctaaaccg cagctacaac cggcatctcg catcgcggcc gcgagaatgg
     5881 gagcacgaag ctatccgtct cttgatcgac ctttgccgcg attatggctg ccacgtccat
     5941 atcgtgcatc tgtcgtcggc ggattccatt ccgctcctgg caagggcacg cgcggaagga
     6001 ctgccgctga ccgtcgagac gtgtcctcat tacctttcat ttgccgccga agagatcccc
     6061 gacggcgacc cacgctacaa gtgtgcccca ccgatcaggg agcgcgaaaa ccgggaacgg
     6121 ctctgggagg gactgcaaca aggcatgatc gacacgatcg gctccgatca ttcgccggcg
     6181 cctcctgagc tcaagcatct ggagacgggg gacgtgtggc gggcctgggg gggcatcgca
     6241 tcgcttcagc tttcgctgcc gatcgtctgg accgaggcac gccggcgcgg gttcccgatc
     6301 gacgagctcg cccgctggat ggcccgagac cccgcgcggc tggtcggcat ctcggacatg
     6361 aagggagaga tcgccccagg tcgcgatgcc gatcttgtcg tcttcgatcc cgacgcaacc
     6421 tttacggtcg aagggacaaa gctataccac cgtcacaaag cgactcccta cgagggacag
     6481 gtcttggccg ggcgcgtcga ggcgacctac ttgaggggcc gcgcggtcta tcgttccggc
     6541 gatttggtcg agacgccatg gggccaaacg cttctggccg tgaattcgag atcgactgga
     6601 gccatttcgt gaacctggaa ctaatcaacg cctggactga tgacgaggcg cgcgagtcgt
     6661 ttcagcggtg ctgcggctcg aggcggtggt tcgaagcgat ggcacgagcc aggccctttg
     6721 aatctgaggc ggctctgatc gaaaccgcgg agcgaatctg gtgggcgctc tcgacggccg
     6781 attggctcga ggcgttcggg gcgcatcccc ggatcggcga cgtggacgcc atacgcgcga
     6841 ggttcgccgc caccgccgcc tgggcaagcc gcgaacaggc aggcgcgctg ggggcttcgg
     6901 aagaagtgct ccagaatctt gcccgaggaa atcgccagta tgaagatcgc ttcggctaca
     6961 ttttcatcgt ctgtgcatcg ggcaagacgg ccgaagagat gctcggactt ctcatgcagc
     7021 ggctcggcaa tgacgccgac gccgagatca aactcgctgc cggcgagcag atgaaaatca
     7081 cgaggattcg cctggaaagg atcgccacat catgagtccg atcacgacgc acgtcctgga
     7141 tacaacgctg ggcaagcctg ggagaggcat tgcggtggtc gtcgagattg gtctcgacga
     7201 cggatcctgg accgagctcg catgcggggt caccgacggc gacggccgca tcgctcaatt
     7261 tagtccgccg ttgaaggctc cgaagcccgg tctgtacaga gtccggtttt tcactgccgc
     7321 ctacttcacg gcgatgcagg tccgagggtt ctatccggag gtcgatgtaa tcgtgcaaat
     7381 cgacgagccg gaccagcact atcatattcc tctgcttttg agcccatttg gttacagcac
     7441 ttatcgtggg agttgagagg ttgatgatgg gcacgccagc gagtttggac ggtccgatgg
     7501 acttgtgaaa attttatatt tatataattt gtccagttgt gattattccc agagggaagc
     7561 gctcgtcgtg ctccattttg tatcggttca ggaatgccag aagctcgtct tgaaatgagt
     7621 gcgtgcgatg atgttcttct tggcgttgaa tgtagagacg aacccgttcc acgtgggact
     7681 ggctcaccga gaaggcaccg tagcccgctt gccaatggaa gccacgaaat tcaggtccct
     7741 gagtcttgag ccatttggac gatccctttt ttagctcttc gatcaccttc gccaaggcgt
     7801 gattcttcga gagacaaaac aagacgtgca cgtgatcggc gacgccgccg atcaggagag
     7861 atgggctcct ccattccttc agaatgcccg atttgtatgc gaagagcccg tcccggatct
     7921 tgggcgagag gccaggcgcg cgatccttgg tgctatagat cagatggatt aggtttatgg
     7981 cgagtgattg aggcatacgt ggtctcgttg ttacgccctt tcagggctgg tcttattggt
     8041 ttggcagagg ttcccctcca gttcgatgcg atgatccccc agggcgtcgc cctgggcttt
     8101 cgtgtttgtg ccccttctgg gcgcagacgt gagggcgacg caatttgaat ttcggcccga
     8161 agggccaatc acatgctagc ccagggcatc gccctggggg accaaacatc gacctggcgg
     8221 accaaacatc gacctggcgg accaaacatc gacatggcgg accaaacgag gccctgggtt
     8281 acctgccacg atcttccgcg tctgccatca tcgatcgccg caccacccga tattgcggcc
     8341 cgatcgttga cggccctggt tgaattctct tgcgtctc
//
LOCUS       DATAQL010000747        11081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_838418, whole genome shotgun sequence.
ACCESSION   DATAQL010000747 DATAQL010000000
VERSION     DATAQL010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838418"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VHS97_12980"
     CDS             complement(<1..559)
                     /locus_tag="VHS97_12980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3449165.1"
                     /translation="MHWSRLVLILLLCFDVWYRGHTFGPTVRDAIGIDLWPASAGASE
                     PLDCDEAAYAYIGHRILRGDVMYRDLTENKPPLGYWLYTLTVALGGYQELAIRVMPIP
                     FVLATIVLLWWVALRLAGPGSACIAAAIFVVLSTDPYLFGNGANLEHFINCFAVASLV
                     SLIRGWDRASRWSLFGSGMCLGAATL"
     gene            complement(632..2512)
                     /locus_tag="VHS97_12985"
     CDS             complement(632..2512)
                     /locus_tag="VHS97_12985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3449166.1"
                     /translation="MTEGPNGRWLPFALLTTAVVATGAWLEIRYGWITPAFDAVAHQF
                     YEVPLVAAIRRPSQLVLVRWHLAIGSGLVVLGLLLAPRANPHGMALCVVMGIGYALRA
                     AIWICGGNLPLVPGDSCHYLEVATSVLRGEGPVKHYVESFFTDYPRIREGQGVLDDWA
                     TPLDAYVRATAFRIAGLGPQSSVEARLVAAKACSFITNLLALPAIYILARRCYNPRIA
                     LWSMAALAILPVHAIYAGFVLRESLVALTSILAVWTLCEVFHAEPSGRAIWAWAAAAG
                     LCGGLAVLSRTTALAILAAAGLYALVVHGRKRFGPLFLWAAIAGLVCLPWAWRTLQEY
                     GSPFYSYTSFFEYNFSWTIHHYDKGNTLPSQFYTLANLPEIARIKVKSLLLIVVYSTM
                     IVGLPIVLGFCRRLGRRSGPGRETDLLVATIFVVVVLATLKSVADVTQVAQLGRYYLP
                     VFALALPAGVAGVLEWLESSNNQMRILPWVAITYCALVWSDPTWAYDASWLVKRYQLH
                     WPGLQEAGEWMRAHPEQVPREARVMTWFPWELRVTSDRTTILMPRNYDPRRIKTVMNQ
                     YGVTHFLWGSFEPPPYYEINPERWALDLEQLRTAIGLTDAREVYRTARDRFFPLRLYR
                     VQ"
     gene            complement(2568..4262)
                     /locus_tag="VHS97_12990"
     CDS             complement(2568..4262)
                     /locus_tag="VHS97_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX3449167.1"
                     /translation="MRRLVPRMVGIVFLVGWFVGAAPDPSLVTALDSIKPGQILDHIK
                     VLSSDDFEGRSPGTPGEEKTVAYLTGQFQKMGLKPGNPDGTFVQNVPLVGFQAKQVTG
                     AFEAGGRTIGLTFPNDFVAVSRRLAEEVKVEKSDVVFVGYGVVAPEYGWDDYKGLDVR
                     GKTLIMLVNDPAVPDPKDPTKLDPALFKGRAMTYYGRWTYKYEIASEKGAAAAILVHE
                     TGPAGYPFEVVKGSWSRENFDIAQPQTSQTPNRVAVEGWITLDKAKELFQAAGRDFDK
                     LKQAAIRRDFKPEVLGGTARFGVTNALREVKSRNVVARIEGTDPSHKNETVIYTAHWD
                     HLGRDPALQGDQIYNGAADNASGVAAILEIARAFSQVKPPPKRSILFLAVTAEEKGLL
                     GAKHYATNPLYPLERTLADINLDVINLWGPTSDIISIGMGNSTLDDLLVEVAQARGRT
                     VAPDADPEKGYYFRSDHFEFAKQGVPALDPKGGRQYVGKSADFGQRKQDEYTAQDYHK
                     VSDQVKPDWDLAGAVEDLKLLVELGYRVTQADRYPEWKPDSEFRAKREAMLKGAKP"
     gene            4489..4764
                     /locus_tag="VHS97_12995"
     CDS             4489..4764
                     /locus_tag="VHS97_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX3449168.1"
                     /translation="MAKIPTIIPVSDLRQDAAALLKELHDSQEPMFITQRGRAAAVLI
                     GIDAYQRAEAQRDILLLLARGEQEIASGVGHTLESVMAEADALLAEK"
     gene            complement(5107..6153)
                     /locus_tag="VHS97_13000"
     CDS             complement(5107..6153)
                     /locus_tag="VHS97_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX3449169.1"
                     /translation="MSEPKKHRWLGTVVNTALVILAFSLLALVIGQNGEMIREVFSRP
                     LDLKLLALAVAIYFMGMVGTFVRWFYLVRVIEPSFRFSATMLLGLIGMVFNLVIPGAV
                     GGDLIKAAYLVRMRIRKTQAVASMVIDRVVGLLGLFILAAIAGGFAWQLAPTDVRELI
                     MAAWVAVGLGVFALAVIFSRVVTRLFPQLKGGHSRLGVIVSELSEMSTTYSGRLDVVV
                     GATALSVWNHALNVVAFYVIGRLLFPTMTTTLVQHFLMVPLTLFSMAVPLPFGALGLS
                     EEVGQQLFKLVGHPSGALAMMGFRVLMYAGGLVGACVYLWNLKEVRALTASAHEIEDE
                     ILEGELNEGEDPAA"
     gene            complement(6218..6814)
                     /gene="hisB"
                     /locus_tag="VHS97_13005"
     CDS             complement(6218..6814)
                     /gene="hisB"
                     /locus_tag="VHS97_13005"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX3449170.1"
                     /translation="MSAPPRIAEIVRKTRETDIRLTVDLDGQGRAEIRTGIGFLDHML
                     ELFARHALVDLAVDCTGDLQVDDHHTTEDVGICLGQAIDRALGDRAGIRRYGHTVLPM
                     DEALVTAAVDLGGRAYWVWDVAMTSSKIGTFDSELVADFWQAVCTQARMNLHVILHHG
                     RNSHHMSEAVFKAVARALRDAFEPDPRCAGVPSTKGTL"
     gene            complement(6894..7943)
                     /gene="hisC"
                     /locus_tag="VHS97_13010"
     CDS             complement(6894..7943)
                     /gene="hisC"
                     /locus_tag="VHS97_13010"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX3449171.1"
                     /translation="MTHFVRPHIARMAGYVPGEQPQGGGFTKLNTNENPYPPSPRVKQ
                     ALVESIGDRLRLYPDPMANEFRRAAAELHGVEPDMVLAGNGSDDLLTILIRAFVGPGD
                     LAGFPSPSYLLYSTLVALQDGQALVVPYSGDWTIPKNALAAPGLKLFFLANPDSPSGT
                     ALKRDQIAELADSLDCPLVVDEAYADFADPRHHALPLLEDHSNVIVTRSFSKGYSLAG
                     IRLGYLIARADLVEQLVKVKDSYNCDMLSQIAGAAGLGDQGYLAQTRAKIVATRGRLT
                     TALRSMGYKVPDSQANFVWATDGPPARETFQRLKDRKILVRLMTYPGYPEGLRVSIGT
                     DAEIDRLLEMLRAII"
     gene            complement(7963..8532)
                     /locus_tag="VHS97_13015"
     CDS             complement(7963..8532)
                     /locus_tag="VHS97_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3449172.1"
                     /translation="MKEDGERCGESVPIADEAPAAPGFLIRPARPEDAELLVTFVHEL
                     AVYEKLEQHAVASPDAFRRHLFGPNPAAEAAVAEVAGEPAGFALWFATFSTFRGQPGL
                     YLEDIFVRPACRGRGIGKALLATVARRAVDRGCGRLEWSVLNWNAPAIGFYRALGARP
                     MDEWTVYRIDDEPLRRLADVAPERFASEF"
     gene            complement(8529..9341)
                     /locus_tag="VHS97_13020"
     CDS             complement(8529..9341)
                     /locus_tag="VHS97_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449173.1"
                     /translation="MAHLTVNSAGPSSQSFAPPERERLEGRRALRLAVVASLFLVFAS
                     LFGAFSIRVGTHLVAAWWFRSLHCFVGWEVHETNWREGGTTSVSYSWKNSWNPKFSDD
                     DLDHLRKLLRVVKLDLAECNTITNAGLAKLRGLDFLTELNLERLNRYRYARYGAPWVP
                     LTNACLVHLQALRGLEKLTLAGNLITDSGLSQIATITNLKTLDLEATEVSDAGLICLA
                     GMKNLKVVNLGATHVTKEGLAKLQMARPDLTIELDIEPAVEEGVKLRRGITP"
     gene            complement(9348..10727)
                     /gene="hisD"
                     /locus_tag="VHS97_13025"
     CDS             complement(9348..10727)
                     /gene="hisD"
                     /locus_tag="VHS97_13025"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX3449174.1"
                     /translation="MALKIRRIDCSCEDAQLAIAALRAELSPKGNVVSHAGKARTIAA
                     FGEPLEPRQVVERICADVAARGVEAALDYTRRIDGVTLEPTQVRVAPGELAEAYRKAE
                     PGYLETIARVRSNILAYQDAIRHHDVMVDRGHGIELGLLHRPIRRVGVCIPGGAAAYP
                     SSLLMTVVPAQAAGVAEIALVVPPSPFGGFNADILAACHALGVTEVYRIGGAQGVAAL
                     AYGLSSIGLEPVDKIVGPGNLFVALAKQHVFGTVDIDSIAGPSEVVLIADRSAVPEFV
                     ASDLISQAEHSPGSGILLTWESGLIDQVATALEEQMARLGRGDLARDSLERFGALILV
                     RDEDEAVAISNQIAPEHLHVSTAEPKRLLLRLTNAGAIFLGHFAPVAAGDYAAGPSHV
                     LPTGGTARWASGLSCNDFLKRSSVIALDRDGLAALAPDIRLLADKEGLSAHRLSVDLR
                     LDHPSVEGA"
BASE COUNT         2268 a         3465 c         3299 g         2049 t
ORIGIN      
        1 ccaaggtcgc tgcacccaga cacattcccg atccgaagag cgaccaacgg ctggcgcgat
       61 cccatccacg aatcaatgaa acaagtgatg cgaccgcgaa acagttgatg aaatgctcaa
      121 gattggctcc attaccgaaa agataaggat ccgtgctcaa gacgacgaaa atcgcggcgg
      181 cgatacacgc cgatcccgga cctgccagcc gcagtgcgac ccaccaaagt agcacgattg
      241 tcgccaggac gaatgggatc ggcatcacgc ggatcgcaag ctcttgatag ccgccgagcg
      301 cgaccgtcag tgtgtagagc cagtatccca gaggaggttt gttttccgtg aggtcacgat
      361 acatgacgtc gccgcgcaag atccggtggc cgatgtaggc gtaggccgct tcgtcgcagt
      421 cgagcggctc gctcgcaccg gccgaagcgg gccacaagtc gatcccgatg gcgtcgcgca
      481 cggtcggccc aaacgtatgg ccgcgatacc agacgtcgaa gcacaagagc aggatcaata
      541 ccagacgaga ccagtgcatc gacggcattt cctactctct ggcggcttca ggaagggttt
      601 tggacctgtc gcgcggtgac gtgatcgtcg tttattgaac gcgatagagg cggagcggaa
      661 agaaccggtc acgcgcggtg cgatagactt cgcgcgcgtc ggtcaagccg atcgccgttc
      721 gtaactgctc gagatcgagt gcccatcgct cgggattgat ttcgtaataa ggcggaggct
      781 cgaatgagcc ccagaggaaa tgcgtcacgc cgtactggtt catcactgtt ttgatgcgcc
      841 tgggatcgta gttgcgcggc atgaggatcg tggtccgatc actcgtgacc ctcagctccc
      901 aggggaacca ggtcatgacg cgggcctctc ggggcacctg ttcgggatga gcacgcatcc
      961 actcgccggc ctcctgcagg cccggccaat gtagctgata acgcttgacc agccacgacg
     1021 cgtcgtaggc ccaagtggga tcggaccaca cgagggcgca atacgttatg gccacccacg
     1081 gaagtatccg catctggtta tttgacgact ccagccattc gagtactccc gcgacgcccg
     1141 cgggcaacgc caacgcaaat accggcaaat agtaccgccc aagctgagcg acctgggtca
     1201 cgtccgcaac gcttttgagc gtcgcgagca cgaccaccac gaagatcgtc gcgacgagca
     1261 gatcggtttc tcgtcccggc ccactccgcc ggccgaggcg ccggcaaaag ccgaggacga
     1321 tcggcaagcc gacgatcatt gtcgagtaaa caacaatcaa gagtagcgac ttgaccttga
     1381 tccgcgcaat ctccggcagg tttgccagcg tgtaaaactg cgagggaagc gtgttgccct
     1441 tgtcgtaatg atggatcgtc caggagaagt tgtattcgaa aaagcttgta taagaataaa
     1501 acggcgatcc atactcttgc aatgtcctcc aggcccacgg taggcatacg agtccggcga
     1561 tggcggccca caagaacaga ggtccgaacc tttttcgacc gtgcacgacc agtgcataca
     1621 ggccggcggc cgccaatatc gcgagcgccg tcgtgcgtga gaggacggcc aggcccccgc
     1681 atagacctgc cgcggcagcc cacgcccaga tggcccgccc gctcggttcg gcgtggaaga
     1741 cctcgcagag cgtccagacc gccaggatcg acgtcagtgc caccaggctt tcgcgcaaga
     1801 cgaatcccgc gtaaatcgcg tgaaccggaa ggatcgcgag agcggccatc gaccagaggg
     1861 caatgcgcgg gttgtaacat cggcgggcca ggatatagat tgccggcaac gcgaggagat
     1921 tcgtgatgaa gctgcacgct ttggcggcaa ccagccgcgc ttccaccgag ctttgcggcc
     1981 ccagtccggc gatccgaaag gcggtcgcgc gcacgtaggc gtccagcggg gtcgcccagt
     2041 cgtcgagtac accctggccc tcgcgaatgc gcggatagtc ggtgaagaag ctctcaacgt
     2101 agtgtttgac tggtccctcc ccccttagca ccgaggtcgc cacctccaga tagtggcagc
     2161 tatcgcccgg aaccagcggc agattgccgc cgcatatcca gatggccgcc cggagtgcgt
     2221 agccgatgcc catgactaca cacaaagcca tgccgtgcgg attcgccctg ggcgccagca
     2281 acagccccaa tacgaccagt cctgatccaa tcgcgagatg ccagcgaacc agaacaagct
     2341 gggatggcct gcggatcgcc gccacgagcg gaacctcgta gaactggtgg gcgactgcgt
     2401 cgaaagccgg tgtgatccag ccgtaccgaa tttcgagcca agcgccggtc gccaccacgg
     2461 ctgtcgtcaa cagggcaaag gggagccagc gaccgttcgg tccctccgtc accctattgg
     2521 ccccttggcg caaggggaac gttgtgtgat cgcgtctccc tgaacaatca tggtttcgcc
     2581 cccttgagca tcgcctcacg cttggcccgg aactcgctat cgggtttcca ttcggggtaa
     2641 cgatctgcct gggtgacacg atagccgagc tcgaccaaga gcttcagatc ctcgacggcg
     2701 ccggcaagat cccaatccgg tttcacctga tcgctcacct tgtggtaatc ctgggcggta
     2761 tattcgtctt gtttgcgctg accaaagtcg gcggatttgc cgacgtattg ccgccccccc
     2821 ttgggatcga gcgcgggtac cccttgcttg gcgaattcga aatggtcgga gcggaaataa
     2881 tagccttttt cagggtcagc gtccggagcg acggttcggc cgcgggcctg ggcgacctca
     2941 acgagcaggt cgtcgagggt cgaatttccc atgccgatgc tgatgatgtc gcttgttggt
     3001 ccccagaggt tgatgacgtc gagattgatg tccgccagag tacgttcgag cggataaaga
     3061 ggattagtgg cgtagtgctt ggcgccaagc aacccctttt cctcagcggt gacggccagg
     3121 aacaagatcg atcgcttggg gggcggcttg acttgtgaaa acgcgcgggc aatttcgagg
     3181 atggcagcca ccccagaggc gttgtccgcg gctccattgt agatctgatc accctggagt
     3241 gccggatcgc gccccaggtg gtcccagtga gcggtataaa tcaccgtctc gttcttgtgc
     3301 gagggatctg ttccctcgat cctggcgacg acgttcctcg acttgacctc tctcagcgca
     3361 ttggtgacgc caaaacgagc ggtgcccccc agaacctcgg gcttgaagtc gcgccgaatg
     3421 gccgcctgct tgagtttgtc gaagtcgcgt ccggctgctt gaaacaactc cttggctttg
     3481 tcgagcgtga tccacccctc gaccgcgacc cggttcggcg tctggctggt ttgcggctgg
     3541 gcgatgtcga aattctcccg gctccaactc cccttgacga cctcgaacgg ataacctgcc
     3601 gggcccgttt cgtgcaccag gatcgccgcg gccgctcctt tctccgaggc gatctcatat
     3661 ttgtaggtcc agcggccgta ataggtcatg gcccgaccct tgaaaagcgc tggatcgagc
     3721 ttggtagggt ctttgggatc gggaaccgca gggtcgttga ccagcatgat cagcgtcttg
     3781 ccgcggacgt cgagtccttt gtagtcgtcc cagccgtact ccggggcgac cacgccgtag
     3841 ccgacgaaca cgacatccga cttttcgacc ttgacctctt ccgccaatct gcgcgagacg
     3901 gccacgaaat cgttgggaaa cgtgaggccg atcgtgcggc cgccggcctc gaacgcgccg
     3961 gtgacctgct tggcctgaaa gccaaccaga ggaacgttct ggacaaacgt gccatcggga
     4021 ttgcctggct tgagccccat tttttggaac tgacccgtca gataggcgac cgtcttctcc
     4081 tcaccgggcg ttcccgggct ccgtccttca aaatcgtctg acgagaggac cttgatatga
     4141 tccagaatct gccccggttt tatgctgtcg agggccgtga cgaggctggg gtcgggagcg
     4201 gcccccacga accatcccac cagaaacaca atgccaacca ttcttgggac gagacgtcgc
     4261 atgatgtcct cacggtcgac aagccggtgc aaccagtacg tgcaagttcc cgatcaacga
     4321 caagagccta atgataagta ccgtagcgtc tgatcgcgac agatcagtat ctgcaaattt
     4381 agtgaccgtt cggcgcgtcg ccgagggcag gatacccatt ttacagcgcg accttgtcga
     4441 tgttgcatcg ctatcgtaca ctcaaacgta ctcttggagg tatgtttcat ggcaaaaatc
     4501 ccgacgatca tcccggtgtc cgatctcagg caagatgccg cggcattgtt gaaggaattg
     4561 cacgattcac aagagccgat gtttattaca caacgaggcc gggcggcggc cgtcttgatt
     4621 ggcattgatg cctatcagcg tgcggaggcg cagcgagata ttctcttgtt gctcgcccga
     4681 ggcgagcagg agattgccag cggcgttggc cacactcttg aaagtgtgat ggccgaggcc
     4741 gacgctctgc tggcagaaaa atgacaatgg agttgaggtt cactccgtcg ggacgggtcc
     4801 agtttctcac cgcaattggg tcgatccgga gagcaaatgc gacagcctcg cggcaattcc
     4861 gccagcgggc cgaaaaagcc ctcaagcgac tccaacgatt tccggcatct ggcgcggttg
     4921 tggcagaatt cccggacttg cccttccgag aagtctacgt gaaaccttat cgattctttt
     4981 acagggtacg cgctagtacg atctgggtgg ttgcggtgtg gcacggtgct caagttcctc
     5041 aaaatccggc cgatgtggcg ggtccgctcg acgattaagg gcctggcacg cgagaacagc
     5101 cgctcgtcac gcagccgggt cctctccttc attcaactcg ccttccaata tctcgtcctc
     5161 aatctcgtgt gccgaggctg tcaaggctct cacttctttc aaattccaaa ggtagacgca
     5221 tgcgccgacc agtccacccg cgtacatgag gacgcgaaag cccatcatcg ccagcgctcc
     5281 gctggggtgt cccaccagct tgaagagctg ctggcccact tcctcgctga ggccaagggc
     5341 gccaaatggg agtggtacag ccatgctgaa gagcgtcaag ggaaccatca gaaagtgctg
     5401 gacgagcgtc gtggtcatcg tcgggaagag cagccggccg atcacataaa aggccacgac
     5461 gttgagcgcg tggttccaca ccgacaaggc ggtcgcccca accacaacgt ccagccggcc
     5521 actgtaggtg gtcgacatct cactcagctc ggacacgatc acccccagac gcgagtggcc
     5581 ccccttcagt tgaggaaaca accgcgtaac gacccgggag aagatcacgg ccaaggcgaa
     5641 aactcccagc cccaccgcga cccaggccgc catgatcaac tcgcggacgt cggtcggggc
     5701 tagctgccag gcgaacccac ccgcgatggc cgccaggata aaaagcccca acagcccgac
     5761 gacacggtcg atcaccatcg acgctacggc ctgagtcttc cggatccgca tacggacgag
     5821 ataggccgcc ttgatcagat cgcctccgac cgcccctggg atcaccagat tgaagaccat
     5881 gccgatcaat cccagaagca ttgtcgcact gaaccggaaa ctcggctcga tcacacggac
     5941 caaatagaac cagcggacaa acgttcccac catgcccatg aaatagatcg caacggccag
     6001 cgcgaggagt ttcagatcga gcggccgact gaagacctcg cggatcatct ctccattttg
     6061 gccgatcacc agtgccaaca gactgaaagc caggatgacc agggcggtgt tgacaacagt
     6121 gccaagccac cggtgtttct tgggttcgct catgagcaac aggtggtcct ctgaagctgg
     6181 tccatggcag atagggtgga tgcgagtcgc cgccgcttta gagcgttcct ttggtcgagg
     6241 gaacaccggc gcaccgcgga tcgggctcaa acgcatcgcg gagggcacgc gccaccgcct
     6301 tgaaaaccgc ctcactcata tgatggctgt tacggccatg gtgcaggatg acgtgcagat
     6361 tcatccgcgc ctgggtacag accgcctgcc agaaatcggc gaccagctcg ctgtcgaagg
     6421 tcccaatctt actcgacgtc attgccacgt cccagaccca gtacgcccgc cccccgaggt
     6481 caacggccgc cgtgaccagt gcctcgtcca tgggaagcac tgtgtggcca tagcgcctga
     6541 tccccgcccg gtcacccagc gccctgtcga tcgcctggcc gaggcagatg cccacgtcct
     6601 cggtcgtgtg atggtcgtcg acttgcaggt cgcccgtgca gtcgaccgcc agatccacga
     6661 gcgcgtgccg agcgaaaagc tcgagcatgt ggtcgagaaa tccgatgccg gtgcggatct
     6721 cggcccgccc ctgcccgtcg aggtcgactg ttagccgaat gtcggtctcg cgcgtcttgc
     6781 gcacgatctc ggcgatgcgt ggaggagcgc tcaaggtcgt tggtcctgga caaaggaagc
     6841 cggtgaatgc gggtcgttcg ccgccgattg taacccaaac gcatctcatt cgttcagatg
     6901 atcgctcgta gcatctccag cagccgatcg atttccgcgt cagtcccgat gctgacgcgc
     6961 aacccttcgg gataaccggg gtaggtcatc agtcgaacca ggatcttgcg gtccttgagc
     7021 cgctggaacg tctcacgagc tggcggaccg tcagtcgccc agacgaagtt ggcctggctg
     7081 tccggaacct tataccccat ggaacgcaac gcggtcgtca gccgtccccg ggtcgccacg
     7141 atcttggcgc gtgtttgcgc caggtagccc tgatcgccca gacccgcggc accggcgatc
     7201 tggctgagca tgtcgcagtt gtacgagtcc ttgaccttga ccagttgttc caccagatcg
     7261 gcccgagcga tcaggtagcc gagccggata cccgccaggc tgtaaccttt gctgaacgag
     7321 cgggtgacga ttacgttaga atgatcctcg agcagcggca gggcgtggtg gcgcggatcg
     7381 gcaaaatcgg catacgcttc gtcgacgacc agcgggcaat cgagcgaatc ggccaactcg
     7441 gcgatctgat cgcgcttgag tgccgtcccc gatggactat cgggattggc cagaaagaat
     7501 agctttagtc caggcgcggc gagggcgttc ttggggattg tccagtcgcc tgagtagggg
     7561 accaccagcg cctgcccgtc ctgcagtgcg accaaggtcg aataaagcaa gtagctgggg
     7621 gatggaaaac ccgccaggtc ccccggcccg acgaacgcac ggatcaggat cgtcaagagg
     7681 tcgtccgacc cattgcccgc cagcaccatg tcaggctcga ccccgtgcag ctcggcggcc
     7741 gcgcggcgga attcattcgc catcggatcg ggataaagcc ggagccggtc gccgatcgac
     7801 tcgacgagcg cctgcttgac tcgcggcgac ggtgggtacg gattttcatt ggtattgagc
     7861 ttggtgaatc cgcccccctg gggctgctcg ccggggacgt agccggccat ccgagcaata
     7921 tggggtcgaa cgaagtgggt catcgaacgc gggtcctctc ggtcaaaatt ctgaagcaaa
     7981 ccgttccggc gcgacgtcag ccagccgtct cagcggctcg tcatcgatgc gatagaccgt
     8041 ccactcgtcc atcggccgcg cccccagggc gcgataaaag ccgatcgccg gagcattcca
     8101 gttaagcacc gaccattcga gccgcccgca gccgcggtcg acggcccgcc tggcgacagt
     8161 ggccaggagt gctttgccaa tccctcgccc acggcaggct ggcctgacga aaatatcctc
     8221 gagatacaga cccggctgac cgcggaatgt cgaaaacgtg gcgaaccaga gcgcgaagcc
     8281 ggctggctcg ccagccacct cggcgactgc ggcctctgcc gcgggattgg gaccgaagag
     8341 gtgccgtcga aaggcgtccg ggctcgccac agcgtgctgt tccagtttct cataaaccgc
     8401 cagttcatgt acaaacgtca ccaagagctc ggcatcctcc ggtcgcgccg gccgaatcaa
     8461 aaacccgggc gctgccggcg cttcatcggc gatcggtact gattcgccac atcgttcgcc
     8521 gtcttctttc atggggtgat tcctcggcgc agcttgaccc cttcctcgac tgccggctcg
     8581 atgtcgagct cgatcgtgag gtctggccgg gccatttgta gcttcgcgag accctccttg
     8641 gtgacatgcg tcgctcccag gttgaccact ttgagattct tcattcccgc aaggcagata
     8701 agaccggcat cactgacctc ggtggcctcg aggtccaatg tcttcagatt cgttatggtg
     8761 gcgatctggg acaatccgct gtcggtgatc aggttgccgg ccagtgtcag tttctcgagt
     8821 ccgcgcaggg cttgcagatg gaccaggcag gcattggtca agggaaccca aggggctcca
     8881 taccgagcgt atcgatatcg gttcaagcgc tccaggttca attcggtcag gaaatcgagc
     8941 ccgcgcagct tcgccagccc cgcatttgtg atcgtattgc attcggcgag atcaagcttg
     9001 accacgcgca ggagcttccg caggtgatcg agatcatcgt cactgaattt cgggttccac
     9061 gaattcttcc aactgtacga caccgaggtt gtgccgcctt ctcgccagtt cgtctcatga
     9121 acctcccagc cgacaaagca atgcagactt ctgaaccacc acgccgccac cagatgagtg
     9181 ccgactcgaa tcgaaaatgc gccaaagagc gacgcgaaca cgaggaacag gctcgcaacg
     9241 accgcgagtc gcaatgctct acggccctcg aggcgttcgc gctcgggtgg ggcaaatgat
     9301 tgagagctgg ggccggctga gttaacggtc agatgagcca cgggcactca cgctccttcc
     9361 accgaagggt gatcgagccg aagatcgacg ctcaaacggt gggcggacag cccttccttg
     9421 tcggcaagca aacggatgtc gggggcaaga gcggccaatc catcgcggtc aagcgcgatc
     9481 acgctggacc gcttgaggaa gtcgttgcac gacagccccg aggcccaccg cgcggtccct
     9541 cccgtcggca gcacatgcga gggcccggcg gcataatcac cggcggcaac gggggcgaaa
     9601 tgacccagaa agatcgcgcc ggcgtttgtg aggcgaagca acagccgctt gggctcggcg
     9661 gtcgaaacgt gtaagtgctc cggcgcgatt tggttggaaa tcgccaccgc ctcgtcctcg
     9721 tcgcgaacca ggatcaaggc gccaaatcgt tccaggctat cgcgcgccag atcaccccgg
     9781 ccgagacggg ccatttgttc ctcgagcgcc gtggcgactt gatcgatcag tcccgactcc
     9841 catgtcaaca agatgcctga gcccggagaa tgttcggcct ggctgatcag gtcggacgcg
     9901 acaaattcgg gtacagccga tcggtcggcg atcaagacca cctcgctcgg gcccgcgatg
     9961 ctgtcgatgt ccacggtgcc gaacacatgt tgcttggcca aggcgacgaa gaggtttccg
    10021 ggcccgacga tcttgtcgac cggctcgagc ccgatcgagc tcaagccata ggccagggcc
    10081 gcaaccccct gcgcaccacc gatcctatag acctccgtta cgccaagagc gtggcaggcg
    10141 gccaggatgt cggcgttgaa ccctccaaag ggggaggggg ggaccacgag ggcgatctcg
    10201 gccacgcccg cggcctgcgc cggcacgacc gtcattagca agctcgacgg ataagccgcc
    10261 gctccgccgg gtatgcagac acccacccgg cgaatcggcc gatgcagcag ccccagctcg
    10321 atgccatgtc ctcgatccac catcacgtcg tgatgccgga tcgcatcttg atacgccagg
    10381 atattgctgc gcactcgtgc gatcgtctcg agataacctg gttcggcctt gcgataagct
    10441 tcggccagct ccccgggtgc gacccggacc tgagtgggct caagcgtgac cccgtcaatc
    10501 cgccgggtgt agtcgagcgc cgcctcgacc ccgcgcgccg cgacgtcggc gcagatccgc
    10561 tcgaccacct gccgcggctc gagcggctcg ccaaacgccg cgatcgtccg cgccttgccc
    10621 gcgtgactga ccacattccc cttcggactg agctccgcgc gcagcgcggc gatcgccagt
    10681 tgggcatctt cacaagaaca gtcgattcgc cggatcttca gtgccaccgc tccaacctat
    10741 cctctaacga caatcgaaaa gatcgtgaca accgatcgtc taccaataac ctcgtcggat
    10801 gttcggccgc aatggctgag ctggattcga cgcgggtcaa agccctactg gaacgctctt
    10861 gtcagcgcga atggcttcgt ttcgcaatac gcgtcagcag aaatcatgtc ctggtgttat
    10921 caactctgaa gtcaaacgct acgtgagact tatgatggac ggccgcggaa cgatcgatag
    10981 tcgaattgcg cctcggtttg tcaatcccat cggacgatcg ttgtcgtgct ggcgtcgttg
    11041 cgtcgacatt gctcagcgtc agattgggtt cgatcggtcg a
//
LOCUS       DATAQL010000748         3463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2977617, whole genome shotgun sequence.
ACCESSION   DATAQL010000748 DATAQL010000000
VERSION     DATAQL010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977617"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..878)
                     /locus_tag="VHS97_13030"
     CDS             complement(66..878)
                     /locus_tag="VHS97_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449175.1"
                     /translation="MSMKVMRLGFLAGLALLFAGVIVGAQDRKDAEPVGRASIDVFNV
                     VEGRTVVITSVPKGARVEKGDIVCELDPSEVRDRVATQEIVVQGAQADVHATRIAREA
                     AVMAVNEYKEGTFLQEFLATEAWIKLAEAELASDEDHLDWVRRMFDKGYVSLAEKVSA
                     ELTLKHARFALELGQSKKKILIDFSKGKMIKALTGEVKAARARELAKLAALERERSAQ
                     KRLTSQIGRCKVTAPASGRIEYAAPIGQGAVVRDGQVLFRIVTIPTAGAKAK"
     gene            complement(1199..2107)
                     /locus_tag="VHS97_13035"
     CDS             complement(1199..2107)
                     /locus_tag="VHS97_13035"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEX3449176.1"
                     /translation="MSTLPDVHIGDAIRHGALSVFPLFGSSPGGVDYLLSDEAISAGS
                     VTVEEVSEGGSVPNLLVTNQGDSRVLFLEGEELRGAKQNRVLNTSVLVAAHSKTPIPV
                     SCVEQGRWRYRSRQFESSRSHSSSKLRHYLKASVSHSLKSGRGHSSDQGAVWHEVSRQ
                     MDSLGTSSDTAAMADTYQSYSARLAEFRDHVRYVDGATGIAVAVGQKVVAMDLFDKPS
                     TCAKVWDRLLTGVVMDALEADSKAPPAEPAEVARLISRLRDSAWEPTTPVGVGQEFRF
                     DADSETHASALVFQDSVLHGSVVVAS"
     gene            2714..>3463
                     /locus_tag="VHS97_13040"
     CDS             2714..>3463
                     /locus_tag="VHS97_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX3449177.1"
                     /translation="MTRLTFVIAPALLLALVPPVRAQDPTARYDAVHKITVGGEGGWD
                     FLEVDAANRRLYVTRGNRVVVIDIDSEKVVGEIADTPGVHGVAFVPGVDRGVTSNGGD
                     SSATVFDTKSLKTLGKVKANWRPDIIYYEPVSRRVFTFNHGSNDTTAIDPGEMKAVGS
                     LALGGVPELAVSDDKGHIFVNMEDTSEIVEIDARTLKLLRRFPLAPGEEPTGLAFDPK
                     QRRLFSTCANRKLVVSDADSGKVLQTLEIGPG"
BASE COUNT          695 a         1103 c          993 g          672 t
ORIGIN      
        1 ctcgcggcga tcattaagaa ggcgcaggcc cagacaccga tcgcagtggc tcgcatggaa
       61 gttcctcatt tggccttggc gccagccgtc gggatcgtga cgatccggaa gagcacttgt
      121 ccgtcgcgca cgaccgcccc ctgcccgatc ggcgcggcgt actcgatgcg gccgctggcc
      181 ggggccgtca ccttacaccg gccaatctga ctggtcagcc ttttctgggc cgaccgctcg
      241 cgctcgagcg ccgccagctt ggcgagctcg cgggccctgg ccgccttgac ctcgcccgtc
      301 agggctttga tcatcttgcc cttggagaag tcgatcaaaa tcttcttctt gctctggccc
      361 agttccaggg cgaaccgagc gtgcttcaag gtcagttccg cggagacctt ctcggccagc
      421 gagacatagc ccttatcgaa catccttcga acccaatcaa ggtggtcctc gtcgctcgcg
      481 agctctgcct ctgccagctt gatccaggcc tccgtcgcga ggaactcttg gaggaaagtt
      541 ccttccttgt actcattcac cgccatgacc gccgcctcgc gtgcgattct agttgcgtgc
      601 acatccgcct gagccccttg cacgacgatc tcctgggtgg cgactcgatc ccggacttct
      661 gacgggtcga gctcgcaaac gatatcgcct ttctcgactc gcgccccctt cggcacgctg
      721 gtgatgacga cggtgcgacc ctcgacgacg ttgaagacgt cgatcgaggc tcgtcccact
      781 ggctcggcgt ccttacgatc ctgcgcgcct acgatcaccc ccgcaaagag aagcgccagt
      841 ccggccagga aacccaagcg catgaccttc attgacatgg ctacctcgct tttggaagtg
      901 tttcttggac cgatcagagt tcatgcctgg ctgtttcggc tcctattagt agatagcgat
      961 aacaccacgt caagtgtccc tccaatcagc atgaacccga gcgaacgcca cgatcaatca
     1021 cgaccacgcg ctgcaagatg acccggtggc tgcgattgac gagctcgtcg cagagctcga
     1081 ccggccaagc ggtgagctgc gcgcccatac ccatgcgtcg gcgagaccat tggctcgccg
     1141 gcagtcaccg ctcaccgtcg tcgacctgga cgctctgcat gatctcaccc gaacgagatc
     1201 agcttgcaac caccacactc ccgtgaagca ccgagtcctg gaagaccagc gccgaagcgt
     1261 gagtttccga gtcggcatcg aagcggaact cttgcccgac accgaccgga gttgtcggtt
     1321 cccaggcgga atcccgcaat ctcgagatga gccgcgccac ctcggctggt tcggccggcg
     1381 gcgcctttga atcagcctcg agtgcgtcca tcaccacgcc ggtcaacagc cggtcccaga
     1441 ccttggcaca ggtcgagggc ttgtcgaaca ggtccattgc gacgaccttt tgaccgactg
     1501 ccaccgcgat tccggtcgct ccgtcgacat acctgacatg gtcccggaac tcggccagtc
     1561 gtgcgctata ggactgatag gtgtctgcca tcgccgccgt gtccgaggac gtgcccaatg
     1621 agtccatctg gcggctgacc tcatgccaga ctgccccttg atcggaggaa tgcccccgtc
     1681 ccgacttcaa ggaatgggag accgatgcct tcaggtaatg gcgcagtttc gaagacgagt
     1741 gggaacggct cgattcaaat tggcgggagc ggtaacgcca gcgaccctgt tcgacgcaac
     1801 tgaccgggat cggggtcttg ctgtgagcgg cgaccaggac cgacgtgttc agcacccggt
     1861 tctgcttggc gccacgaagt tcttcgcctt cgaggaacag gacgcgcgaa tctccttggt
     1921 tggtcacaag gaggttcgga acggagcccc cctcgctgac ctcttcgacg gtaaccgacc
     1981 ccgccgagat tgcctcgtcg gacagaagat aatcaacacc ccccggagag gaaccgaata
     2041 agggaaagac gctgagggcc ccgtgccgga tggcatcacc aatatggacg tctggtagtg
     2101 tggacatggg acgctccttg ttcgcgaaac acaagatgag ctggttattc cagcctcgca
     2161 taatgcatag gatccgaaca gaggtatcgg cgtgcgttcc gatcgctaaa aaaagtacgt
     2221 ccgcaaaaac gcacccacga tggggatcga tggaatggtc caggtggcca acacccacat
     2281 ccctagccac tcggcccacc aaccccggaa tgcccacaaa tttcgggggt tggccgctga
     2341 gcgtacagcc cacgaaaagg gccacgcacg cgactggcca gaaatggtag ctgagattgc
     2401 gctaaatcgc gtttacattt cgtaattttg ctcgcatttg gacatgcctg acaagatact
     2461 gtgaccccaa ccgtcatgac gttcccgtct catgtagtgg cagacgtttt ggcccccgtg
     2521 cagtgatccg gctcatcttg aaagtgtcac cagttcttct ggaaagcttc atgtgaagcc
     2581 gcacaattgc tgaaggcaac ttggtatgac ggtggcacaa tatgcggggg ggggctcgcg
     2641 tcggcgctgc aaacggtcga actgattact tgacgtagga tgagttttca acaattcgag
     2701 gagaatcacc actatgacac ggctcacatt cgtgatcgca cccgctttgt tattggcact
     2761 ggttccacct gtacgagcgc aggatccgac ggcccgctac gatgcggtcc acaagatcac
     2821 ggtcggcggc gagggcggct gggactttct cgaagtggac gccgccaacc ggcggttgta
     2881 cgttacgcgg ggcaaccgcg tcgtcgtgat cgacatcgac agcgagaagg tcgttggtga
     2941 aatcgccgat acgccgggcg tgcacggcgt cgccttcgtt cccggcgtgg accgcggcgt
     3001 caccagcaac ggaggagact ccagtgcgac cgtcttcgat accaagtcgc tcaagaccct
     3061 gggcaaggtc aaggccaatt ggcgtcctga catcatctac tatgagccgg tcagccggcg
     3121 tgtcttcacc ttcaatcacg gcagcaacga cacaacggca atcgaccctg gcgagatgaa
     3181 ggcggtcggc tccctcgcgc tgggcggcgt ccctgaactg gccgtctccg acgacaaggg
     3241 acacatcttt gtcaacatgg aagacaccag tgagattgtc gagatcgacg cccgcacgct
     3301 gaagctcctg cggcgttttc cgctcgcccc cggtgaagag cccacaggcc ttgcctttga
     3361 ccccaagcag cggaggctct tcagcacctg cgccaatcgt aagctcgtcg tttccgacgc
     3421 cgacagcggt aaggtcctcc agacgctcga gattggtccg ggc
//
LOCUS       DATAQL010000749         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4503983, whole genome shotgun sequence.
ACCESSION   DATAQL010000749 DATAQL010000000
VERSION     DATAQL010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4503983"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VHS97_13045"
     CDS             <1..537
                     /locus_tag="VHS97_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HEX3449178.1"
                     /translation="MGSGPMRAAAGSEAIYDVIGFRETADAVVGVLETRKPPPPAIVA
                     RSVETALHKLAELKFDLSRVISAHGTAPLPPVAATDLAAIGRTNDAVLYGARVVLAVT
                     GDDASLVALGPQVPSSSSRDYGEPFAAIFARYNNDFYAVDPHLFSPAEVVFQNVETGK
                     THAFGGVNSEVLARSFHS"
     gene            580..1473
                     /locus_tag="VHS97_13050"
     CDS             580..1473
                     /locus_tag="VHS97_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEX3449179.1"
                     /translation="MASLVALVSGLGWHVEDLRRAAGLLGVSFHAVPFPRVSAVVGTA
                     PANARVSAGDLDLTAADGVLVRMMPPGSLEQVVFRMDALHRVAATGVPVLNPPRAVET
                     AVDKFLTLALLEAAELPVPSTWTGQSAAEALAAFEELGGDVVVKPLFGSEGRGLMRIS
                     DRELAWRTLHALERVGAVLYLQRAIRHPGHDYRVFVLRGEVVGVMRRRAIKGEWRTNV
                     SLGGRAEPCRLDPEAERLALAAARAIGAEMAGVDLIPDLDRGELVVLEVNAVPGWRAL
                     ARVAGIDIALAVLAALKDSGR"
     gene            1484..>1654
                     /locus_tag="VHS97_13055"
     CDS             1484..>1654
                     /locus_tag="VHS97_13055"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEX3449180.1"
                     /translation="MAKQPARLGRSHSTPSKLAPGRLAQIACLMEVTARKPGNVHRLA
                     DLPDLHFVDFLLS"
BASE COUNT          249 a          518 c          557 g          330 t
ORIGIN      
        1 atgggttctg ggccaatgcg ggcagcggcg ggtagcgagg cgatttacga cgtcattggg
       61 tttcgcgaga cggctgacgc ggtcgtcggc gtgctcgaga cccgcaaacc gccgccgccg
      121 gcgatcgtgg ccaggtcggt cgagaccgcc cttcacaagc tggccgagct caagttcgat
      181 ctgtcgcgcg tcatttcggc ccacggcacc gcccccctcc cgccggtcgc ggccaccgat
      241 ctggcggcga tcggccgcac caacgacgct gttctctacg gcgcccgcgt cgtgttggcg
      301 gtgaccggtg acgacgccag cctggtcgcc cttggccctc aggtcccctc ttcgtcctcg
      361 cgcgactatg gcgagccgtt cgcggcgatc ttcgctcgtt acaataacga cttttacgcc
      421 gtcgatcccc acctgttcag tccggccgag gttgtcttcc agaacgtcga gaccggcaag
      481 acccacgctt tcggaggagt caactccgag gtgctggcac gctcgtttca ctcgtaatcc
      541 ggtcgtgaac actcgaatcg atctcctgaa gtcccactga tggcatcgct ggtagcgctg
      601 gtttccggac ttggctggca tgtcgaggat ctgaggcgcg cagccggcct gctgggtgtg
      661 tcgtttcatg ccgtgccgtt tccgcgcgta agtgcggtcg tggggaccgc acccgcgaat
      721 gcacgcgtca gtgcgggcga cctcgatttg acggctgccg acggtgtctt ggtgcggatg
      781 atgccgccgg ggagtctgga acaggttgtt ttccggatgg atgcgttgca ccgagtcgcg
      841 gcgacgggcg tgcccgttct caacccgccg cgggccgtgg aaactgcggt cgacaagttt
      901 ctgacactgg ctctgctcga ggcggccgag ctgccggtgc catcgacgtg gacagggcag
      961 tcggccgccg aggcgctggc agcatttgaa gagctcgggg gtgacgtcgt cgtcaagcct
     1021 ctttttggct ccgagggacg cggactgatg cggatcagtg atcgagagct tgcctggcgg
     1081 acgctgcacg cgctcgagcg cgtaggtgcc gtcctgtatc tccagcgcgc gatccgtcac
     1141 ccgggccacg actatcgagt ctttgtgctg cggggcgagg ttgtgggagt catgcggcgg
     1201 cgtgcgatca agggtgaatg gcgcacaaat gtgtcactgg ggggccgcgc cgagccctgc
     1261 cgtttggatc cagaggctga gcgccttgcg ttggccgcgg cccgtgcgat cggagccgaa
     1321 atggcgggcg tcgacctgat tcccgatctc gaccgcggag agctcgtcgt gctcgaggtc
     1381 aatgcggttc ctggctggcg cgcgctggcg cgcgtcgccg gaatcgacat cgccttagca
     1441 gttctcgctg cgctcaaaga ttcaggaagg tgatggtgcg gcgatggcaa aacagcctgc
     1501 gcgactcggt cgaagccatt caaccccttc aaaactggct cccggccgac tggctcagat
     1561 tgcatgcctc atggaggtca cggcgcgaaa gccgggaaac gtgcaccggt tagccgacct
     1621 tcccgatctc catttcgtcg attttctcct gagc
//
LOCUS       DATAQL010000750         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1298247, whole genome shotgun sequence.
ACCESSION   DATAQL010000750 DATAQL010000000
VERSION     DATAQL010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298247"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VHS97_13060"
     CDS             <1..1150
                     /locus_tag="VHS97_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX3449181.1"
                     /translation="RSYRLGVQTVCGTVFASAIVCSIWVLLNPPKSPPAAQDLGAAAL
                     PLGEFQLQERSGRTVRETDLNERVWIASFIFTRCPLSCPRISGVMKGLQERLVKTNVL
                     LVSISVDPDHDTPSVLTEYAGRFGASGDRWWFLTGPKPSTYDLVQNRFKLALGETPLP
                     DMTSDTEAISHSDRLALVDHGRVAGFFESSDTRALDELVASARWRAQPSWVRRLPTVN
                     ASLNALCAVFLIAGWMAIRRRRAPLADLISQAGGESRRPVTLLDQPAVRAHLVLMLLA
                     FATSSIFLACYLVYHFQAGSMPFRHGGAIRVVYFTILLSHTFMATFGVVPLVIMTLLR
                     AARQDFPGHARIARVTFPIWLYVSITGVVIYLLLYRLPVATSLSQPRL"
     gene            1637..>2224
                     /locus_tag="VHS97_13065"
     CDS             1637..>2224
                     /locus_tag="VHS97_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449182.1"
                     /translation="MCELLESRELLSTLVVSNTTDAAVPIPNSLRWAIDNVNADSTPD
                     TIQFAIPGSGVQSIRLISALPPIVNTVAIDGSSQPNYMGSPLIQLDGSTLGPGSDGLV
                     ISAGQSTVMGLAVVGFSGSAIVLNAPGGDVIEANYLGVTAAGGQASPNGTGITVSSSA
                     SNTIGGPAGQANVISGNSGNGIVIQTGSKSADGTFI"
BASE COUNT          411 a          657 c          642 g          514 t
ORIGIN      
        1 caggtcctat cgactcggtg tgcagactgt ttgcgggacg gtctttgctt cggcaatcgt
       61 gtgctcgatc tgggtgctat tgaatcctcc caagtccccg cccgcggctc aggatcttgg
      121 agcggccgcg ctgccgctcg gcgaatttca gttgcaggaa cgatctggcc gcacagtccg
      181 ggaaaccgat ctgaacgagc gcgtctggat cgcctcgttc atattcaccc gatgtcccct
      241 gtcctgtcct cgaatttcgg gcgtaatgaa aggattgcag gaacggctcg tcaagaccaa
      301 cgttttactg gtaagtatct cggtcgatcc cgatcacgac acgccgagcg ttctcaccga
      361 atacgcgggc agattcggcg catctggcga ccgctggtgg ttcttgacgg gacccaagcc
      421 gtcgacctac gacctcgtcc agaatcggtt caagctcgcg ctcggggaaa cgccactccc
      481 cgacatgaca tccgacaccg aagcaatctc tcatagtgac cgcctcgccc ttgtggacca
      541 tggacgggtc gccggcttct ttgaatcgag cgacacgcgt gcgctcgacg aattggtcgc
      601 tagcgcgcgt tggcgcgcgc agccgagttg ggtgcgtcgc ttaccgaccg tcaacgcgag
      661 cctgaacgcc ctgtgtgcgg tcttcttgat cgccggctgg atggcgatcc ggcgccgccg
      721 tgctccgctc gccgatctaa tctctcaagc cggcggtgaa tcgcgccgtc ccgtgactct
      781 cctcgatcag cctgccgtgc gagcgcatct tgtgcttatg ttgctggcat tcgccacgtc
      841 gtcgattttt ctggcatgtt acctggtcta tcacttccag gcagggagta tgcccttccg
      901 ccatgggggt gcgataaggg tggtgtattt taccatcctg ctgtcgcata cttttatggc
      961 gacattcggg gttgttccgc tcgtaatcat gacgttgctc cgtgcagccc gccaagactt
     1021 tcccgggcac gcgcgcattg ctcgagttac ttttcccatc tggctttatg tttcgattac
     1081 cggcgtcgtt atctatctct tgctttaccg tctccctgtg gcgaccagct tgagccaacc
     1141 tcggctctaa cgataatcca atctgcccat ttcgcccttt ccaacgatgt gacaagttta
     1201 atttgtcgtc atcttcagta aggttgcccg atttcacgca tcaactcatt cattcatgcg
     1261 gttgatggtg gggctcatcc ttgacatcac gggcgtcaca ttgcaacaat ttaggtcacg
     1321 aacagcaaaa atgacgccta ttctcttagt aggggtggga tcgctgccag gcgaccggtt
     1381 gagcgtcatc gcgaactgag ccaggatcgt gacgacgcga tcgaaccgaa agtcatgggg
     1441 tttgtaaaaa ggaaaatgtg tcagtgccac tccgagaacg gactcgcgcg ttgcctgcgt
     1501 tcgagacgat caacttgtga cccgcttggg aattggcttt ttattaagcc ggtcgatagc
     1561 aaacaccgca atgattcgcg acgagcgcat ttgccgacgc caaactcgcg gatcgaggcg
     1621 aacctcgatc cgacttgtgt gtgagttgct cgagagccgt gagctgctct cgaccctcgt
     1681 cgtttcgaac accactgacg cagccgtccc tataccgaat tcgctgcggt gggcgatcga
     1741 taacgtgaat gcggacagca ctccggatac gatccagttt gccattcccg ggtcgggagt
     1801 gcagtcgatc cgcctgattt cggcgttgcc gcccatcgtc aacacggtcg cgattgatgg
     1861 gtcgtcgcag ccgaattaca tggggtcgcc gctcatccag cttgatggat cgacgctggg
     1921 tccaggcagc gacgggctgg tcatttcggc agggcaaagt acagtcatgg ggctggcggt
     1981 cgtggggttc tccgggtccg caatcgtcct caatgcgccg gggggcgatg tcattgaggc
     2041 caactacctg ggagtgaccg ccgcgggggg ccaggcgagc cccaacggaa cggggatcac
     2101 ggtcagttcg tcggcgtcca atacgatcgg cggcccggcc ggccaggcca atgtgatctc
     2161 cggtaattcc ggaaatggga tcgtgatcca gaccggcagc aaatcagcgg acggcacctt
     2221 catt
//
LOCUS       DATAQL010000751         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2825432, whole genome shotgun sequence.
ACCESSION   DATAQL010000751 DATAQL010000000
VERSION     DATAQL010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825432"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VHS97_13070"
     CDS             complement(<1..202)
                     /locus_tag="VHS97_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3449183.1"
                     /translation="MRALTETAADLFLEKGFEALSLDDLIARVGGSRRNIYGRFGNKE
                     GLFVAIVTSLCEELSAPLKSLDI"
     gene            669..1247
                     /locus_tag="VHS97_13075"
     CDS             669..1247
                     /locus_tag="VHS97_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449184.1"
                     /translation="MAVILGVAIAITSVLLPLSVYPGLPSELAFLALISSPFWMPAAC
                     ISVWVFVDLTQQFPDLLGPPPGRRRWLVTAALVLVLNCCLLWCGAPRRLAFLHALPAF
                     EGSVASAPPAPSDGERFDRRFGVYHVDRFAADPRGGCYFRTHCGPDGFYTRKMTHGFS
                     YSPNQAGTPFGDEKYALSHVVGDWYAFQASER"
     gene            1634..2842
                     /locus_tag="VHS97_13080"
     CDS             1634..2842
                     /locus_tag="VHS97_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3449185.1"
                     /translation="MSIDERPAAESTYEMLPQQEEPTVAWRPSTEPSATIGKSRWYQR
                     GFGHVAVKYLVNFCRQFASYLDAGINLYRALESLRRQYEWTALGPVIGRLQLAVRRGQ
                     SLTDAMSREPAAFDYYFLSMMRVGEVRGSVPETLRLLSVEYESRQRMVRQTKSALIYP
                     AFVIFVSVTVSALLTILVLPPLVNIMQEVIHERHVDLPLPTRLLIRISDFISDGGWWG
                     IPLTLVGTVAFFVGAYRLERGKALLDVVLMRLPIVGQLLWKIDTTRFCRTLATLLDAG
                     VDIGSSLELTADTLFLVPMRRAVLQTRNSIIRGESLSASIELTNRFPPDIPALMNSGE
                     ETGRLPEMMLMVADQYEEQVEFIVKNLGTLLQPVITVVVGGVVFFIAVAFFMGYAAVL
                     ANLGQSKVIQ"
     gene            3028..3384
                     /locus_tag="VHS97_13085"
     CDS             3028..3384
                     /locus_tag="VHS97_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449186.1"
                     /translation="MAKSGLTGMYAIPTQTSAKFPQEYLERTISQIKVGEHVVVEASA
                     LAVDSDLNCWLRPDICIGSDSVALSSSVPCVAVTRDVRGFVVSLHPSRGRRWKPGPKP
                     EPINGVEWIPVFELKY"
     gene            complement(3404..>4126)
                     /locus_tag="VHS97_13090"
     CDS             complement(3404..>4126)
                     /locus_tag="VHS97_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3449187.1"
                     /translation="KVSFRTLGQAMAEPEGWGKIADRPEWGLFKFGHTDPNKSNSGLQ
                     ALVLMAYEFAGKQRGLTVKDITEERFQDWLQSFERGITRHGRSLSHSTGTLMEDMVLR
                     GPSQYDCLLVYENLAIDYMEAAVQRWGTEGEFYVAYPNPNVWNEHPYYILDVPWSDDR
                     QKKAAADFLKFLMSEPVQRRALEHGFRPGNSVVPVNSPESPLVRNQKYGLKINLPVMS
                     EPPSADVTKNLLESFQRLEPGR"
BASE COUNT          696 a         1301 c         1314 g          815 t
ORIGIN      
        1 gaatatcgag cgacttgagc ggggctgaca gctcctcgca aagcgaggtc actatcgcca
       61 cgaagagccc ttccttgtta ccgaaccggc catagatgtt gcgccgcgat ccgccaaccc
      121 gagcgatcag gtcgtcgagt gacagggcct cgaagccctt ttccagaaag aggtccgccg
      181 ccgtctcggt cagagcgcgc aatcttgccg ctccgcgagc cgttcgatgt gcttgttcac
      241 ccataattgt tacctcagtt gtcaggtatg gtactatacc gtacctacga cgattggcaa
      301 gctcaagccc tcacagtctt gagtgcgatt cgaaaaactc tgtgagcgac aacgaccggt
      361 tcagcaatca gaacgcgttg tgcagaacgt tattacgcag gatgtgaaca agggaaacga
      421 ctcggttccc caatgaggta gacctttctg gcccgcgact gaccgcccgt cccgaaagtg
      481 ggtagcccat tctggcccgc gccgggtgcg gacgggggtg tcgtcccccc agggggattg
      541 gccggttttc gccgcgtgag ttgctgccgt agaataccgg gcccggggtc ggtcgctccc
      601 gggagggtgc cggcgccggg ggcccgcact tctgggggcg gtccgcgcgg gggaaacagg
      661 catcgcacat ggccgtcatc ctgggcgtcg cgatcgcgat cacctccgtg ctcctgccgc
      721 tgagcgtcta ccccggtctc ccctccgagc tggccttcct cgcgttgatc tcctccccgt
      781 tctggatgcc cgcggcctgc atttcagtgt gggtattcgt tgatctgact cagcagtttc
      841 ctgacctgct cgggccgccc ccgggccgac ggcgctggct cgtcaccgcc gcgctggtgc
      901 tcgttttgaa ctgctgcttg ctttggtgcg gcgcgcctcg ccggttggcg tttctccacg
      961 cgctgccggc gttcgagggg tccgttgcct cggcaccacc ggccccctcc gacggcgaaa
     1021 ggttcgaccg gcgtttcggg gtttaccatg tggaccgatt cgcggccgac cctcgcggag
     1081 gctgttattt tcgaacccac tgcggccccg atggtttcta cacgcgcaaa atgacccacg
     1141 gattctccta tagcccgaac caggcaggga ccccgttcgg ggacgaaaag tacgccctct
     1201 cccacgttgt cggcgattgg tacgctttcc aggcgtccga gcgctgagcc gaatgcactc
     1261 ccgttcgttc ggtcgcctcg gacggcagtc cgcggccgat cctcggcgcg tgccttcttg
     1321 gtaaatgcac gcgaaattca cttgatattc atggcacacg aatcgtgctc ggtgttcaac
     1381 gtacgtataa tcgttggctg aacaaccagg gcccgaggcg cccgagatcg cgttcgtctc
     1441 ccggttcgtt tgcgtgcgac catgattctg gacggtgtgg cggcgacctt cattgcggcg
     1501 gtcccgccgg ccaaaatgtg ggccaagggc gagcggccgc cagcggcgtt actttcaaca
     1561 gatcagcaga ccgatcaaca aaggcacaac tggcgccggc cgcgaccccc gcgcccctcg
     1621 aagggcctcc cggatgagca tcgacgagcg tccagccgcg gaatcgacgt acgagatgct
     1681 cccgcagcaa gaggagccca ccgtggcctg gcggccttcg accgagcctt ccgccacgat
     1741 cggaaagtcg aggtggtacc agcggggctt tggccatgtc gccgtcaagt acctcgtgaa
     1801 tttctgtcgg cagttcgcca gctatctcga cgccgggatc aacctgtacc gggcgctgga
     1861 gagcctcagg aggcagtacg aatggacggc actcgggccg gtgatcgggc gactgcagct
     1921 tgcggtgcgg cgggggcaga gcttgaccga cgcgatgagc cgcgagcccg ccgcgtttga
     1981 ctattacttc ctgagcatga tgcgcgtagg cgaggtgcgg gggtcagtcc ccgagacgtt
     2041 gcggttgctc tccgtcgagt acgaatcccg ccagcgtatg gtccgccaga caaagtcggc
     2101 gttgatatac ccggctttcg tgatcttcgt gtcggtcacg gtgtccgccc tgctgacgat
     2161 cctcgtcctg cccccgctgg tcaacatcat gcaggaagtg attcacgaga ggcatgtcga
     2221 cctcccgttg cccacacggc tgctgatccg catcagcgac ttcatctcgg acggggggtg
     2281 gtggggcatc ccgttgaccc tggtcggcac ggtcgctttc ttcgtcggcg cctaccggct
     2341 cgaacgggga aaggccctgc tcgacgtggt cctaatgcga ctgcccattg tcggccagct
     2401 cctgtggaag atcgacacga cgaggttttg ccggacgctc gcgaccctgc tggatgccgg
     2461 cgttgatatc ggttcgtcac tcgagctgac ggccgacaca ctgttcctgg tcccgatgcg
     2521 ccgggccgtc cttcagacgc gcaattccat catccgggga gaatccttga gcgccagcat
     2581 cgagctcaca aatcgcttcc ctcccgacat cccggcgctc atgaattccg gcgaggagac
     2641 ggggagactc cccgaaatga tgttgatggt agccgatcag tatgaagagc aagtcgagtt
     2701 catagtcaag aatctcggaa ccctcctcca gcccgttatc acggtcgtgg tcgggggtgt
     2761 cgtctttttc attgcggtcg ccttcttcat gggctacgcg gccgtgctgg cgaacctcgg
     2821 acagtcgaag gtgatccagt gagtaaagtg gcccgcagcc ccggtggtcg cgcgggcgat
     2881 cgatcgggat gtgtacgcgc ggggtgccga cacaccgatc cgtggtgccg gtttccccaa
     2941 cgagccggcg cgaggtaaaa tgctctcagg gccggacggt cgtggtgacc cgggcgggcc
     3001 cttaacccag cgatcggagc ggacgacatg gcgaagagcg gcttgacggg gatgtacgcg
     3061 atcccgaccc aaacgtccgc gaagttcccg caagagtacc tcgaacggac catctcccag
     3121 atcaaggtgg gagagcacgt cgtcgtcgaa gcctcagccc tggcggtcga cagcgacctg
     3181 aactgctggt taaggcccga tatttgcatc ggttcggatt cggtcgcgct ctcctcctcc
     3241 gtcccttgcg tggccgtcac gcgggacgtc cgtggcttcg tggtcagtct gcacccgagc
     3301 agggggaggc ggtggaagcc cgggccgaag ccggaaccga tcaacggagt tgaatggatt
     3361 ccggtcttcg agctgaaata ctgagagggg ccccggagtc ggttcagcgg cccggttcga
     3421 gccgttggaa cgactcgagc aggttcttgg tcacgtcggc cgacgggggt tcgctcatga
     3481 cggggaggtt gatcttcagc ccgtacttct ggttacggac cagcgggctt tcaggcgagt
     3541 tgaccgggac gacggagttg ccgggccgaa acccgtgctc gagcgcgcgg cgctggaccg
     3601 gctcgctcat caggaacttg agaaagtccg cggcagcctt cttctgccgg tcgtcgctcc
     3661 aggggacgtc gaggatgtaa tacgggtgct cgttccagac gttcgggttg ggatacgcga
     3721 cgtagaattc cccctcggta ccccagcgct gaaccgcggc ctccatgtaa tcgatggcga
     3781 ggttctcgta gaccagcagg cagtcgtact gtgacgggcc ccggagcacc atgtcctcca
     3841 tgagagtgcc agtgctgtgg ctgagagacc tgccatgccg ggtgattccg cgctcgaacg
     3901 actgcagcca gtcctgaaac cgttcctcgg tgatgtcctt gacggtcagc ccgcgctgct
     3961 tgccggcgaa ttcgtaggcc atcagcacga gcgcctgcag gccgctgttg gacttgttgg
     4021 gatcggtgtg gccgaacttg aacaggcccc actccggccg atcggcgatc ttcccccaac
     4081 cctccggctc ggccatcgcc tggccgagcg tgcggaagct gacctt
//
LOCUS       DATAQL010000752         7755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1602987, whole genome shotgun sequence.
ACCESSION   DATAQL010000752 DATAQL010000000
VERSION     DATAQL010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602987"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VHS97_13095"
     CDS             <1..849
                     /locus_tag="VHS97_13095"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3449188.1"
                     /translation="SRTTLGVAQVPGDSRLEITCVAYSDLAEKKRIGDPPPNLPFSPG
                     ILAGDTLYISGKGDQLNGGGHPATFDEQVRQAMRNVEATLKQAGLDFRHVVMSNIFLD
                     RYENLEVTDKVYNEFFEDGTEPAASTSFVDWIPGGSHVEITCIATTDLSARKVVRPAG
                     LKYGLLEGAVTGSPGVWAGNTLYVSGLGASPTGGTLSAGVGDQVHQMAKSHVKVLDEA
                     GLKLDDIIAGFVYLSDMHDYAPMNTVYKEYYSRGPGVRTCLMPGTGIDKTGVRVRGAF
                     VAARTR"
     gene            complement(871..2172)
                     /locus_tag="VHS97_13100"
     CDS             complement(871..2172)
                     /locus_tag="VHS97_13100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449189.1"
                     /translation="MAGTSGIEESRAVGRLRRTRTIALAMLVITGVINYVDRSTLSIA
                     EKPICEELGLSKGEFGILLSAFSWSYAFAQLPTGALVDKVGPRILLAVGLTLWSVAQA
                     CGGLVSSMRQFVATRIALGIGEAPQFPTGARVVSNWYHVRERGLPTGLFNSSSSLGPA
                     LAPPLLTSLMVTFGWRRMFFIMGAAGVIAALAWYVFYRDPQRAGLSEADLRSLRADEP
                     ASSGDLSLAQWLRLFQHPTTWGMVLGFAGALYLIWLYLTWLPGYLRIQHHMTTLQVGF
                     AASVPFIFGFLGSLSGGAFSDRLAARGISPINSRKLPIAAGLIGMALFTVPAALSHDG
                     NVAVGCIALAMFCGAAATANAWALVTATAPSSYVASLGSIQNFGGYFGGSFAPVITGY
                     VVDKTGSFTLPLLIGAAVAVASALVYLFVVREPISAAFLDG"
     gene            complement(2196..4175)
                     /gene="asnB"
                     /locus_tag="VHS97_13105"
     CDS             complement(2196..4175)
                     /gene="asnB"
                     /locus_tag="VHS97_13105"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX3449190.1"
                     /translation="MCGIAGAIDLTGTREFAHQRLLSMTGAIAHRGPDDEQVHIEPGV
                     ALGARRLSIIDLAGGRQPIANEDGSIWVAFNGELFEYPELRKQLLHGGHQLATRCDTE
                     AWVHLYEDDREGMFARARGQFAVSLWDRRERTLILGRDRVGICPLYYTQADGWLLWGS
                     EIKALLASGLVAARPDPRGIDHLFTFFCSGTTRTFFEGIKSLPPGQLLKVRDGRVTQH
                     RYWDLDFPDAGSERRLDDPAPLVAELEALLEQAVERRLRSDVPVVSYISGGLDSTVVL
                     GFCSRHRGEAIPAFTVGLDKAGPDERAHSAEAAQILGSPLTTVTMDRSGIASAFPELI
                     RAAEGPVLDTSCAALLRLAESVHQQGYKVALTGEGADEALAGYVWYKSQKIRDSVTSR
                     IGRRPARLVRKLFMSSIAGPRLHLPPELGIGGTRPAQQDLYELISQAKPALYSGEMWR
                     RLGDHSPYADLDITNDNMTRWHPLNQSLYVGYKVMLAGLLMISKGDRIAMNASVETRY
                     PYLDDDVISFCAGIAPEYKLRGMTEKWLLRQVAARILPPRIANRPKTMFRACMSGSFL
                     GPHRPAWVDQLLSPESLSSTGYFDAAAVARQRNWQVRMPRITPARGVYDVALTCVVST
                     QLWHHIFCGGGLCDLPTWQPPARRHGVRPRNLTGA"
     gene            complement(4411..5271)
                     /locus_tag="VHS97_13110"
     CDS             complement(4411..5271)
                     /locus_tag="VHS97_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX3449191.1"
                     /translation="MKITVAAVQMPSELLDVPANLQRADELLRSAHDSGVELAVLPEL
                     FNTGYSLCPDFGPYSETDEGPTLAHLRQRSRQWQMAIVAGFVEREGRHLYDSLAFCAP
                     DGDVHIYRKRNLVFWERFRFHPGRAPLVVTTPWGRMGLAICADMIYRRVWADYQGRID
                     LAVVSAAWPDFADRDSGRRHWLFGHVGPLSGAIPAKVALDFGIPVIFANQCGETHTTI
                     PVLGTQIRDRFAGKSSICDGRHGEPVLAGREPSVVIAPITLHQQRGLKSWRFMSPSAP
                     AASYSASVPS"
     gene            complement(5362..5823)
                     /locus_tag="VHS97_13115"
     CDS             complement(5362..5823)
                     /locus_tag="VHS97_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX3449192.1"
                     /translation="MEKLRAKGRAAVALSLLAALSMFSGCVERRYTVRTDPPGAIVVV
                     NGEVIGPSPASKSFIFYGDREITMMLDGYETKTVMQPIRAPWWDNLLTEFFTENLVPV
                     SLRDEREFKYQLTPAQSPPLGELRDRAEALRSEARVLPKPRRGGILGWLGF"
     gene            complement(6065..7024)
                     /locus_tag="VHS97_13120"
     CDS             complement(6065..7024)
                     /locus_tag="VHS97_13120"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX3449193.1"
                     /translation="MSTVTRAAKSTLSPYEAGIVQEIAAWKSLPPNPFSELFDMIAMP
                     VADLIEKVIPDNLVRAAIAKSYDLSEVLAGQEDIKRQAGVRELSELLKKPLEGCDRLA
                     FQVSAAAQAWATVEGAATGAGGVLTTLIDVPLMFVLALRTILKIGHCYGYALDRAADR
                     HFVLGILIAASSSSLQVKRKRLEELKQIEEMLLEETQEEILAEEALSLLFQLEIFEEV
                     PGVGMISGALLNLLFIRRVDRTARHVFQERWLAENGKVDVIEPATVHARRVAGGLGGV
                     FGRMTYSGLYSLSYGAALPVHFVVSLFRSIDNPMTRGTRVATT"
BASE COUNT         1601 a         2381 c         2382 g         1391 t
ORIGIN      
        1 tcgcgtacga cgcttggcgt cgcccaggtt ccgggagact cgcgcctgga gatcacctgc
       61 gttgcataca gcgatctcgc cgaaaagaag cgaatcggcg atccgccacc gaatttgccg
      121 ttcagtccgg ggattttggc cggcgatacg ctctatattt cgggcaaggg cgaccagctc
      181 aatggcgggg gccatcctgc gacctttgac gaacaggtgc ggcaagcaat gcgtaacgta
      241 gaggcgacgc tcaagcaggc tggtctcgat ttccgccatg ttgtcatgag caacattttc
      301 ctcgaccgct atgaaaacct cgaagtcacc gacaaggtct acaacgagtt tttcgaggat
      361 ggcaccgagc cggctgcgtc gacttcgttt gtcgactgga tcccgggcgg atcgcatgtg
      421 gagatcacgt gcatcgcgac gaccgatctg tccgcgcgca aggtcgtgcg gccggccggc
      481 ctgaaatatg gactgctcga gggagccgtg acaggaagcc cgggtgtgtg ggcgggtaac
      541 acgctttacg tctctgggct cggcgcttct cctacgggcg gcacactatc agccggagtc
      601 ggcgaccaag tccatcagat ggccaaaagc cacgtcaagg ttttggatga ggctggactg
      661 aaactcgacg acattatcgc cggattcgtt tacctgagcg acatgcacga ctacgcgccc
      721 atgaataccg tgtacaaaga gtattattcg cggggaccgg gagttcgaac gtgtttgatg
      781 cccggcacgg gtatcgacaa gaccggtgtg cgcgtccggg gggcgttcgt ggcggcgcga
      841 acgaggtaaa cgtaagtcgg ccggaattct ttagccgtcc aggaacgcgg ccgagatcgg
      901 ctcgcggacc acgaagagat acacgagcgc cgacgcgacc gcgacggccg ctccgatcaa
      961 gaggggcaac gtaaaagagc ccgttttatc cacaacgtat cctgtgatca caggtgcgaa
     1021 cgaaccgccg aagtatcccc cgaagttctg gatcgagccc agcgaagcga cataactgga
     1081 cggggccgtc gccgtgacca gtgcccatgc attggccgtg gcggcggcac cacaaaacat
     1141 agccaaggca atgcacccga cagccacgtt gccatcgtgg ctcaaggccg cgggcacggt
     1201 gaacagcgcc attccgatga ggccagccgc gatcgggagt ttccggctgt tgatcggtga
     1261 aatgccccgg gccgccagac gatccgagaa ggcccctcca gacagcgatc ccaggaagcc
     1321 gaagatgaac ggaacagagg ctgcgaatcc gacctgaagg gtggtcatgt ggtgttggat
     1381 cctcaggtag cccggcagcc aggttagata gagccaaatt aggtagagag caccggcaaa
     1441 gccgagcacc atgccccatg tcgtcggatg ctggaacagg cgcaaccact gggcgagcga
     1501 gaggtctcca ctcgaagcag gttcatcggc gcgcaacgac cgtagatcgg cctcggacag
     1561 ccccgcgcgc tgtgggtcgc gataaaaaac gtaccacgcc aaggcggcga taacgccggc
     1621 ggcacccata atgaaaaaca tgcgccgcca gccaaatgtc accatgaggg acgtcagtag
     1681 aggcggcgcc aaggccggcc ccaggcttga cgacgagtta aaaagtcccg tcggtagccc
     1741 gcgctcgcgc acgtggtacc agttgctgac cacgcgcgca cccgtcggaa actgaggcgc
     1801 ctcgccgata cccagcgcga tgcgcgtggc aacgaactgc cgcatgctcg agacgagccc
     1861 gccgcacgcc tgcgccactg accacagtgt cagccccacc gcgagcaaga tccgaggacc
     1921 gaccttgtcg accagcgcgc ccgtgggtaa ctgcgcaaat gcgtagctcc acgagaatgc
     1981 cgacaacaag ataccaaact cgccctttga gagtcctagc tcttcgcaga tcggcttttc
     2041 ggcgatcgag agcgtgctgc ggtcgacata gttgatcacg ccggtgatca caagcattgc
     2101 cagtgcgatc gttcgcgtcc gccgcaatcg acccaccgcg cgcgactctt cgattccgct
     2161 ggttccggcc atctcgtatc ctttgattca acgcctcacg ccccggtcaa attcctgggc
     2221 cgcacgccgt gccgccgcgc cggcggctgc caggtaggaa ggtcgcagag gccaccaccg
     2281 cagaagatat ggtgccagag ctgtgtcgac acgacacagg tcagggccac gtcatacacg
     2341 cctcgcgcgg gcgtgatccg cggcatgcgc acttgccagt tgcgctggcg tgccaccgcc
     2401 gccgcgtcga aataacctgt ggagctcagc gactccgggc tcaacaactg gtcgacccac
     2461 gccggccggt ggggacccag gaacgacccc gacatgcagg cgcgaaacat cgtcttggga
     2521 cgattggcaa tgcgcggcgg caagatccgc gcggccacct ggcgcaagag ccatttctcg
     2581 gtcatcccgc gcagcttgta ttccggcgcg atgccggcgc agaacgagat cacgtcgtca
     2641 tccagatacg gatagcgggt ctcgaccgac gcgttcatag cgatccggtc gcctttggaa
     2701 atcatcaaga gaccggccag catgaccttg tagccgacat acagtgactg gttgagcgga
     2761 tgccagcgcg tcatgttgtc gttggtgatg tcgagatcgg catagggact atggtccccg
     2821 agtcgtcgcc acatctcgcc cgaatagagc gccggctttg cctgcgatat cagctcgtaa
     2881 aggtcttgct gggcaggacg ggtgccgccg atacccagtt cgggaggaag atggagccgc
     2941 gggccggcga tgctgctcat aaaaagcttg cgtaccagcc gagccggtcg gcggccgatg
     3001 cggctggtca cggaatcacg gattttttgc gacttgtacc agacatagcc ggccaaggct
     3061 tcgtcggccc cctcgccagt caaggcaacc ttgtaccctt gctgatgcac cgactcagcc
     3121 agccgcaaga gggccgcaca cgacgtgtcg agaaccggtc cctcggcggc ccgtatcaac
     3181 tcaggaaagg cgctggcaat tcccgaccga tccatggtca ctgtggtcaa aggcgagccg
     3241 agaatctgtg cagcttctgc cgaatgcgcg cgctcgtccg ggccggcctt gtcgagcccg
     3301 acggtgaagg cagggatcgc ctcgcctcga tggcggctgc aaaaaccaag gacgaccgtc
     3361 gagtcaagac cgccgctgat gtaactgacg accggcacgt cgctccggag cctgcgctcg
     3421 acggcttgct cgagcagggc ctcgagctct gcgaccaaag gggccgggtc gtcgagcctg
     3481 cgttcggagc cggcatcggg aaagtcgagg tcccagtaac ggtgctgggt aacgcgtccg
     3541 tcacgaacct tgagcaattg acccggtggc agcgacttga taccctcgaa aaacgtgcgg
     3601 gtcgtccccg agcaaaagaa cgtaaagagg tggtcgatgc ctcgagggtc gggccgcgcg
     3661 gccaccagtc ctgacgcgag gagcgccttg atctccgagc cccagagtag ccagccgtcg
     3721 gcctgcgtgt agtagagcgg gcaaattccg acccggtcgc ggcccaggat cagagtgcgc
     3781 tcgcggcgat cccagagcga cacggcaaac tgaccgcgag ccctggcaaa catcccctcg
     3841 cgatcgtctt cgtagagatg gacccacgcc tcggtgtcgc aacgcgtcgc cagttgatga
     3901 ccaccatgca atagctgttt ccgcagctcg ggatactcga aaagctcacc attgaaggcg
     3961 acccagatcg agccgtcttc attggcaatc ggctgtcggc cgccggctaa gtcaatgatc
     4021 gacagccgcc tggcgcccag tgccactccc ggctcgatgt ggacctgttc gtcgtcgggt
     4081 ccgcgatgag cgatcgcccc ggtcatcgac agtaggcgct ggtgggcaaa ctctcgagtt
     4141 ccggtcaaat caatcgcgcc ggcgatacca cacatggaca tacctcagtt gcaggaaatc
     4201 gccgatggtg aaagcattat tcgatgtggc acgggtttcc agcccgtgtc cgatgagtcc
     4261 acggactgga agcccgtgcc acggagttgc cccgcctgaa tgtgaagtcg tatcagtccg
     4321 ggatcgtgat ggtcaagggc tgaggggggc gcatcatcaa agcgcggcgg cggcaggccc
     4381 gaccgtagac gaggccgccc cagatgccga tcacgaaggt accgaggcgg aatacgaggc
     4441 cgcgggggcc gagggggaca taaaacgcca cgatttcagt cctcgctgct ggtggagagt
     4501 gatgggagct atgactacag agggctctct gccggcgagc acgggctcgc catgacggcc
     4561 gtcgcagata ctgctcttgc cggcgaaccg gtcgcggatt tgagtgccca ggacgggaat
     4621 cgtcgtgtgg gtctcgccac actgattggc aaaaatcacg gggatgccaa aatcgagggc
     4681 gactttcgcg ggaattgcgc cggagagggg gccgacgtgg ccgaacagcc agtgcctgcg
     4741 acccgaatct cgatccgcga agtcgggcca ggcggccgat accacggcaa ggtcaatgcg
     4801 accctggtag tctgcccaca ccctgcgata aatcatgtcg gcgcagatgg ccaggcccat
     4861 ccggccccac ggcgtggtca cgactaaagg cgcccgccca ggatgaaatc gaaagcgttc
     4921 ccagaacaca agattacgct tacggtagat gtgcacatca ccgtcgggcg cgcagaaggc
     4981 gagcgaatca tacaggtgcc gcccctcacg ctcgacgaat cccgccacga tcgccatctg
     5041 ccactgccgg ctacgctggc ggaggtgcgc cagagtgggg ccttcgtccg tctcgctata
     5101 cggcccaaag tcagggcaga ggctgtagcc ggtattgaaa agttcgggaa ggacggcgag
     5161 ctctacaccc gagtcatgag ccgagcgcag caactcatcg gcccgctgca gattagcggg
     5221 gacgtcgagg agttcggaag gcatctgcac cgcggccaca gtgatcttca cggccgttca
     5281 cctcgctcgg acgatcgaat gtcgttcttc tttttagatc ggctcgatcg aggggttagt
     5341 ttgacgagag ttgaatttct cttaaaagcc gagccagccc aaaatgcccc cacgacgcgg
     5401 cttcggcagc acgcgcgcct cgctacgcag ggcctcggcg cggtcgcgca gctcacccag
     5461 cgggggtgac tgcgcgggcg tcagttgata tttaaattca cgttcatctc gcaacgacac
     5521 cggaacaagg ttttccgtga aaaattcggt caacagattg tcccaccatg gagccctgat
     5581 cggttgcatg accgtcttgg tctcgtaacc gtcgagcatc attgtgattt ctcggtctcc
     5641 ataaaagata aagctcttcg aggccggtga cggaccgatg acttcgccgt tgacaacgac
     5701 gatcgctccc ggcggatcgg tgcgcaccgt gtagcgccgt tcgacgcacc ctgaaaacat
     5761 actcagggcg gccagaagcg acaacgcgac cgccgcgcga cctttggctc ggagcttctc
     5821 catccttggc tctcccgcca ttggaatcca accgcaatcc atgcatcctc ccggccatga
     5881 gtccaacgga ccgagtagcc aagggagact ccgcccaacc gcgtccttgg tcgcggtgtt
     5941 gaccattccc gggagtgtga cgactagagc gcgagccggc tcgatcatcg agaattcagg
     6001 gtcggacgga acgcggggga ttgtatcgac cgtgtcgatt aagggcaagc cggcttgagg
     6061 ctcattacgt cgtggcgacg cgggtgccgc gagtcattgg gttgtcgatc gatcgaaaca
     6121 gcgagacaac gaaatgcacc ggcaacgccg ctccgtagct gagcgaatac aacccggagt
     6181 acgtcattcg tccgaatacc ccgcccaatc cgccggcaac acgtcgtgcg tgcactgtcg
     6241 ccggctcgat gacgtcaacc ttgccatttt ccgctagcca acgctcctga aagacgtgtc
     6301 gcgcggttct gtcgacgcgg cggatgaaaa gcaggtttaa aagcgcgccg gagatcatgc
     6361 cgacgccggg cacttcctcg aaaatttcga gctggaacaa gagggaaagc gcttcctcgg
     6421 cgagaatttc ttcctgcgtc tcctccaaca gcatctcttc gatttgtttg agttcttcca
     6481 gacgcttgcg cttgacctgg agcgagctgg aactcgccgc aatcaatata cccagaacga
     6541 aatgccggtc cgctgctcga tcgagagcat agccatagca gtgcccgatt ttgaggatcg
     6601 tccttagcgc caagacgaac atcaggggaa catcgatcaa tgtggtcaag acgccgccgg
     6661 cacccgtcgc cgcgccttcg acggttgccc aggcctgcgc cgcggcactc acttgaaagg
     6721 ccagccggtc gcagccttcg agtggctttt tcaagagctc gctgagctcg cgcacgcccg
     6781 cctggcgctt gatatcttcc tggccggcga gaacctcgga tagatcgtac gacttcgcga
     6841 ttgccgcacg gacaagattg tcggggatga ccttttcgat caagtcagcc accggcatcg
     6901 cgatcatgtc aaaaagctcg gaaaacgggt taggcgggag cgacttccag gcggcgattt
     6961 cctgaacgat cccggcttcg tacggcgata aagtcgactt tgcggctctg gttacggtgc
     7021 tcattggaat ttcccggccg ggaggagttt ttctctttcg tcaacgcgcg accaccaaaa
     7081 caaggaaccg gacagggacg ctctatgcga agtccatgcc gcagctccgt ctcggggtca
     7141 agccgattaa ttatacgttt tcgagccaat caacgtcacg agacccgcgc cgaacggcgt
     7201 cggcgatttc gccttgaccg gtttggaggc agggggcata ctgcgacatc cccgacgctt
     7261 tagcctgaga cggattctta ggatcacgtt gcacctatgc gagtcatgga agacgagaca
     7321 aaccgtgctt tgccgcatcc tgttcgtgaa cgagcgctcc acgcactctc ctctcgcccg
     7381 accgggtcgt cgagaatgct cgtgtctaca gttctcggcc cgccggcgac tgccttcgtt
     7441 tccggctacg gggcccacgg cctcgatttg ccgaacgttc cattggcgca atcactctgg
     7501 cgccgcgacc gggcgaccgc cagttccgat tccttcccgg ctcgccctag cgggttgggg
     7561 ccgatgccga agcatgcggg ctgacggtgc tcactggatg agagtgctgg ggaagcggcc
     7621 tcgtaccatc gatcgtgtca ccgggagatg atcttgcgga tgatgatctc caatacgtac
     7681 ttgatgatct ttgccgtggc gatgagcggt tgcgtgctgg cgactccgct tgtgacctgg
     7741 gtagcgacct gggtc
//
LOCUS       DATAQL010000753         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2825613, whole genome shotgun sequence.
ACCESSION   DATAQL010000753 DATAQL010000000
VERSION     DATAQL010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825613"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1310)
                     /locus_tag="VHS97_13125"
     CDS             complement(93..1310)
                     /locus_tag="VHS97_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX3449194.1"
                     /translation="MSTAETPEAIITTPAQLKGLVDHLRESARFAFDTEFVSEETFEP
                     VLCLIQVATRERLAVIDPLAVRDVGPFWEVVLDPAVQVVMHASGEDLRICMLRTGSLP
                     QRVFDVQMAAGMVGFNYPLSLVNLVGQVKDISLSGTETRTDWRHRPLSPAQLRYALDD
                     VRHLLDLADQFADRLTQLGRTEWSNAEFDDLLESIAHRADLDRWRKLPGLHQLSRRSL
                     EMARRLADWREDEARRLNRPLRQVVRDDLLVAIAKRQPRNRRDLEALRDFNRPALLNR
                     SQEILAILDHARAVPDDQLPDLPARPEEPPGLSTVTNLLSAALAQCCAQNQLAGSLVA
                     NVSDLKHLVRWHSGGRDHSKPPSLLQGWRGEVCGPLLLDVLDGKLALRVVDPDSEYPV
                     ALEKLAAKPRPSD"
     gene            complement(1313..2092)
                     /locus_tag="VHS97_13130"
     CDS             complement(1313..2092)
                     /locus_tag="VHS97_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3449195.1"
                     /translation="MDLAYADEGPGPVVMLLHGFPLSRAMWVDQLSGIGSIYRVIAPD
                     LRGHGESPVPEGVYTMDAMADDVVELLETLEISGPVVVGGLSMGGYVALSLAARYPTR
                     VRALMLMDTKAAPDTPEAAQGREAMAQAVIAADSAAPVVDAMLPRLFAKMTREQHPER
                     VEPMKDVMSRTSPRGIAGALRGMAARPDRRADLPKIVVPALVLVGDEDVITPVAESKS
                     MAEALPNARLEVIRQAGHLAPYENPSDANAAILRFLKTLEN"
     gene            2370..2690
                     /locus_tag="VHS97_13135"
     CDS             2370..2690
                     /locus_tag="VHS97_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449196.1"
                     /translation="MPTPNELYDQASDLRDQGDKEGAVAKLEEAVVIDPAFALAHGML
                     AKLYADLASAEKAIEHAKKVVELEPDDAFSYTALSVVYQRCGRIPEAEHAKAMAYQKQ
                     MGFD"
     gene            2741..4108
                     /locus_tag="VHS97_13140"
     CDS             2741..4108
                     /locus_tag="VHS97_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3449197.1"
                     /translation="MIQRKCSACGVEWGALLLMVAAAATLRGGEYSPSSNRADAQTSS
                     AGDPWLGAQLGDLVALYTHFHTHPELSRQEVETSKRVAFELKKAGAEVTTGVGKLGVV
                     GLMKNGDGPVVLVRTDMDALPVVEETGLPYASKVKTRDSSGREVGVMHACGHDIHMTS
                     FVGTARWLSDHRDRWAGTVVLIGQPAEEAVDGAKAMLEDGLYSRFPKPDFALALHCRP
                     DIPVGAVSYCSGPAMASSTSVSVILRGKGGHGAWPHRTIDPIVLAAFCVVDLQTIVSR
                     EIEPGEPAVVTVGSIHGGTKHNIIPDEVKLQLTLRSFSEPVRQQLIAGIKRRAIALSQ
                     AHQAPEPTVEVEEGTPATINTPALVERVVPALAEALGAGCVNPVKPVMGAEDFSLYSE
                     GNVPILMLWLGTLSPERLAAATENGGSPPALHSSKYYPEPGPSISTGIRAMTAAVVKL
                     LPPKR"
     gene            complement(4181..>4579)
                     /locus_tag="VHS97_13145"
     CDS             complement(4181..>4579)
                     /locus_tag="VHS97_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449198.1"
                     /translation="IANEKRTIYLAVLPTTLVPLKGKDFKLEAAGEEKVDDKSAVAIK
                     VTGPDGKDFKLFFDKESGLPVKLVARVIGFGGDEFTQETTYTNYKEFDGAKKATKLSS
                     KRDGEKFIDQEVTDFKLIYKVPDDTFSEPK"
BASE COUNT          872 a         1360 c         1493 g          854 t
ORIGIN      
        1 ctgacgattc gcttcatggt tgtaggccgt gcctccggcg gtcgggattc tatattgatg
       61 aggttttgag cacatgaatt accaacagct gttcagtctg atgggcgtgg cttggccgcg
      121 agtttttcga gtgcaacggg atattcgctg tcggggtcga cgacgcgcag ggctaatttg
      181 ccgtcgagta catcgagcaa gagcggtccg cagacttcgc cgcgccagcc ctgcaggagg
      241 gaagggggct ttgaatggtc gcggccgccc gaatgccagc ggaccaggtg cttcaaatcg
      301 ctgacattgg cgacgagcga gccggccagt tggttttgtg cgcagcactg ggccagcgcg
      361 gccgagagca agttggtgac ggtggagagg ccaggtggtt cttcgggtcg cgcgggcagg
      421 tcggggagct ggtcgtcggg taccgcgcgg gcgtggtcca agatcgccaa gatttcctgg
      481 ctgcggttca agagtgccgg gcggttgaag tcgcgcaggg cttccaggtc acggcgattg
      541 cggggctggc gcttggcgat ggccaccaag agatcgtcgc gcacgacctg tcgcagcggg
      601 cggtttagtc gcctggcctc gtcttcgcgc caatcggcga gccggcgggc catctcgaga
      661 ctccggcggc tgagttgatg caacccgggc agcttgcgcc agcggtctag gtcggctcgg
      721 tgggcgatcg actcgagaag gtcgtcaaac tcggcgttgg accactccgt ccgtccgagt
      781 tgagtgaggc ggtcagcaaa ttggtcagcc aggtcgagca ggtgccggac gtcgtcgagt
      841 gcatagcgaa gttgggccgg cgagagcggc cgatgccgcc agtctgtgcg ggtttcggtg
      901 ccggataagc tgatgtcctt cacctggccg accaggttga cgagcgacaa tggatagttg
      961 aaaccgacca ttcccgcggc catctggacg tcaaaaaccc gctgcgggag cgacccggtc
     1021 cgcaacatgc agattctcag atcttcaccc gacgcatgca tgacaacctg caccgccggg
     1081 tcgagaacga cttcccagaa gggaccgacg tcacggacgg cgagcgggtc gatcaccgcc
     1141 agccgctcac gagtcgccac ctgtatcagg cagaggaccg gttcaaaggt ttcttcgctg
     1201 acaaattcgg tgtcaaaggc gaagcgggcg ctctcccgca gatgatcgac gagccctttc
     1261 aactgggctg gcgtcgtgat gatcgcttcg ggagtttcgg cagtggacaa tatcagttct
     1321 ccagggtttt caagaaccgg aggatggcgg cgttggcatc tgacggattc tcgtaggggg
     1381 ccaggtggcc cgcctggcgg atcacctcca gccgcgcatt ggggagggct tcggccatcg
     1441 acttcgattc ggccacgggg gtgatcacgt cttcgtcgcc gaccaagacg agggccggga
     1501 ccacaatctt gggcaggtcg gcgcgacggt cgggccgagc ggccatgccg cgcagggcac
     1561 cggcgattcc ccggggtgac gttcggctca tgacgtcctt catcggctcg acacgctcgg
     1621 gatgttgttc gcgcgtcatc ttggcaaaaa gacgaggcaa catcgcatcg acgacgggcg
     1681 cggcactgtc ggcggcgatg acagcctggg ccatcgcctc gcggccctgg gccgcttcgg
     1741 gggtatcggg cgcggctttc gtatccatca acatgagcgc acggacgcgc gtcggatagc
     1801 gcgctgccag cgaaagtgcg acataacctc ccatcgatag cccgccgacc acgaccggcc
     1861 cgctgatctc aagggtctcc agcagctcga cgacgtcgtc ggccatggcg tccatggtgt
     1921 agacgccctc ggggaccggt gattcaccat gtccccgcaa atccggcgcg atcacccggt
     1981 aaatcgagcc gattccggaa agctggtcga cccacatcgc ccggctgaga ggaaacccat
     2041 gcagcaacat cacgaccggg cctggtccct cgtccgcata tgccaaatcc atcgaagttc
     2101 tccctggcaa ccaagttggc tagagaatca cacgacgacc gacaaagcac tttccgtacc
     2161 acgggtcgaa gccgccgggc aaagggcggc gtgagcacgg tccggccacc cggacggatc
     2221 cacggttttg ccctcgcacc ctattaatca tcccgctcgg cggccgaacc gtcaacgtgg
     2281 ggatgcggcg agctatggcg gggggccgga tcgacgcctt agaatactcg tggactgaca
     2341 acacgcacca agagatagga ttcgctgcaa tgcctacccc aaacgaactt tatgaccagg
     2401 cgtccgacct gcgggaccaa ggggacaagg aaggggccgt cgccaagctc gaggaagcgg
     2461 tcgtgatcga tcccgctttt gcgctggcgc acggcatgct cgccaagctg tatgccgatc
     2521 tggcgagcgc cgaaaaggca atcgagcacg ccaagaaggt cgtcgaactc gagcccgacg
     2581 acgctttcag ctataccgct ctgtcggtcg tttatcagcg gtgcggacgg atccccgagg
     2641 ccgagcatgc caaggcgatg gcgtaccaga aacaaatggg attcgattga tcgatcgatc
     2701 gggttgacac cggcatgaca aatctaggga gaagaagcgg atgatccaga gaaagtgcag
     2761 cgcctgcggc gtggagtggg gtgctttgct cctgatggtc gcggcggcgg cgaccctgcg
     2821 cggcggagaa tactcaccca gctcgaaccg ggcggacgcg cagacgtcaa gtgccggcga
     2881 tccgtggttg ggcgcacaac ttggcgacct ggtcgctctc tacacccatt ttcatacgca
     2941 cccggagttg tcgcgccagg aggtcgagac ctcgaagcga gtcgccttcg agctgaaaaa
     3001 agccggcgcg gaagtgacca cgggcgtggg caagctcgga gtcgtggggc tcatgaagaa
     3061 tggcgacgga ccggtggtgc tggttcgcac cgatatggac gcgctgcccg tcgtcgaaga
     3121 aacaggcctc ccgtatgcca gcaaagtcaa gacgcgcgac agttcggggc gcgaggtcgg
     3181 cgtgatgcac gcctgcggac acgacattca catgaccagt ttcgtcggca cggcccggtg
     3241 gttgtcagac catcgcgacc gctgggcggg cacagtcgtc ctgatcggtc agccggctga
     3301 agaggcggtt gacggtgcca aagcaatgct cgaagacggc ctctattcgc ggtttcccaa
     3361 gcccgacttc gccctcgcct tgcactgccg gcccgacata cccgtcggag ccgtgagtta
     3421 ctgctccgga ccggcgatgg cgagctcgac ttcggtcagc gtcatcttac ggggcaaggg
     3481 aggccatggt gcctggccgc accgcacgat cgatccgatc gtgctggctg ctttctgtgt
     3541 ggtcgactta cagacgattg tgagtcgaga aatcgagccg ggcgagcccg cggtcgtgac
     3601 ggtaggatcg attcacggcg gcaccaagca caacatcatc cccgacgaag tcaagcttca
     3661 gctcaccctg cgatcgttca gcgagccggt ccgtcagcag ttgatcgcgg gaatcaagcg
     3721 tcgcgcgatt gcgctgtcac aagcccatca agccccggag ccgacggtcg aagtcgagga
     3781 aggaacaccg gcgacgatca atacgcccgc cttggtcgag cgcgtcgtgc cggccttggc
     3841 cgaggccctg ggcgccggct gtgtcaatcc ggtcaagccg gtcatgggag ccgaagattt
     3901 ctcgctatac agcgagggaa acgtgccgat cctcatgctt tggcttggga cgctgagtcc
     3961 ggaacggctc gcggcggcaa ccgaaaatgg cgggtcgccg cctgcgcttc actcgtcgaa
     4021 gtattacccg gaaccgggcc caagcatctc gaccggcatt cgcgcgatga ccgcggcggt
     4081 cgtcaagctg ttgccgccga aacgttgaat cattgaaaag ccggcgggcc cactttagtg
     4141 ggaccgaatt cccgctcggc tttttttgcg atttgcaaga tcacttcggt tccgagaaag
     4201 tgtcgtcggg taccttatag atgagcttga agtcggtgac ttcctggtcg atgaacttct
     4261 ctccgtcgcg tttactgctg agcttggtcg ctttcttggc gccatcgaac tctttgtaat
     4321 tcgtgtacgt cgtctcctgg gtaaattcgt cacccccgaa gccgataacc ctggctacga
     4381 gcttgacggg taaaccgctc tccttgtcaa aaaagagctt gaagtccttg ccgtcgggcc
     4441 cagtgacttt gattgcgacg gccgatttgt cgtcgacctt ctcttcgccg gccgcctcga
     4501 gtttgaagtc cttgcctttg agcggaacga gagtcgtggg aaggacggcc agatagatgg
     4561 tgcgcttctc attggcgat
//
LOCUS       DATAQL010000754         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2825646, whole genome shotgun sequence.
ACCESSION   DATAQL010000754 DATAQL010000000
VERSION     DATAQL010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825646"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VHS97_13150"
     CDS             complement(<1..723)
                     /locus_tag="VHS97_13150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449199.1"
                     /translation="MPARLDRLPWSRWHWKVIVALGITWVIDGLEVTLVGAISPILEH
                     PETLHFSSTQNGLLNTAYLIGAVTGALIFGYLTDLWGRKKLFTITLGLYLSAAFLTAW
                     AWDFWSFALFRCLTGAGIGGEYAAINSAIDELIPARLRGRVDLAINGTFWVGAAAGSL
                     ATVVLLDPRIIAINLGWRLGFGIGALLGLVIIFVRNSIPESPRWLMTRGKKEEAEQIV
                     AEVEQAIESEPGMSKLPPPDRTI"
     gene            complement(900..2159)
                     /locus_tag="VHS97_13155"
     CDS             complement(900..2159)
                     /locus_tag="VHS97_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449200.1"
                     /translation="MQQKIVHAGVNSPSYQQASRDLAALGDLAVEPKPIERLVHRIGR
                     ERTDQRDTAVAAHAALPLMEKDAAANPQRPCPAVAMVSVDGGRLQVRTPLPSQASEPL
                     QESSDPLPKASEPARKGHWRESKVAVLETYLSAVSDVDPDPDVPRCFLDLKRTAEMVR
                     GLGHALPTGLEFAVEDRIEEPDEATATGRDRAARPGRPERLVRSVLASRACSDDFGPM
                     VHQAAWERNFFGAARRVFLGDGLPVNWSIQREHFSTFTAILDFVHALSYVFAGAFAGR
                     PQGEGEMVYKRWIQAVWSGQVATILPELEARSAALGTPPPQCAETDPRQLVYDALRYL
                     RNNAERMHYDEYRRQGLPIMTSAVESVIKMINRRVKGTEKFWSEGGAEAILQLRADYL
                     SETAVLPLFWSEREKQASAGRPYRRVA"
     gene            complement(2182..2559)
                     /locus_tag="VHS97_13160"
     CDS             complement(2182..2559)
                     /locus_tag="VHS97_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449201.1"
                     /translation="MGKDAAAIRKEFQDRLGQMARELGRELYPDGLPRGTKFSELEDV
                     AGALGDEMARQLIEIHIRGQAEDWPEEELGECPLCGGAARKAPNEPRVVTTTRGDVAW
                     KERVGNCPRCRRAFFPSEPGAGP"
BASE COUNT          470 a          914 c          816 g          473 t
ORIGIN      
        1 gatcgtccga tcggggggcg gcaatttgct cattcccggc tcggattcga tcgcctgttc
       61 gacctccgcc acgatctgct cggcttcctc cttcttgcca cgggtcatca gccagcgggg
      121 actctcggga atcgagttgc ggacgaaaat gatcacgagt ccgagcagcg cgccgatgcc
      181 gaagccgagc cgccacccca ggttgatcgc aataatgcgc gggtcgagca aaaccacggt
      241 ggcgagcgag cccgccgcgg cccccaccca gaaggttccg ttaatggcca ggtcgacgcg
      301 gccgcggagg cgggccggaa tcaattcgtc gatggcggag ttgatcgccg cgtactcgcc
      361 gccgatcccc gcgccggtaa ggcagcggaa gagggcgaaa ctccagaaat cccaggccca
      421 ggccgtcaga aaggcggcag agaggtaaag cccgagcgtg atcgtgaaca gcttcttgcg
      481 tccccagaga tcggtcagat agccgaagat gagcgcgccg gtcaccgcgc cgatcaggta
      541 ggccgtgttc agcaggccgt tctgcgtact cgagaagtga agcgtctcgg ggtgctcgag
      601 aatcgggctg atcgcaccga cgagcgtgac ctcgagcccg tcgatcaccc aggtgatgcc
      661 cagggcaacg atcaccttcc agtgccagcg gctccagggc agccggtcga gccgcgcggg
      721 cacatcggtt ttatatgacc tagactcaac ttgggcgtgc tgtgcgaggg ccatactcgg
      781 ctactcgcaa accgtattcc tcgcgctcac gtgagggaac ggtggccagt ttttgctccc
      841 catccgcgaa ctgatccaag cacgcgtggg cgcacgacac ggtatgaatt gacgaacgtt
      901 tatgccaccc gtcggtacgg gcgaccggcg ctcgcctgct tctcgcgctc tgaccagaat
      961 agtggcaaga ccgctgtctc gctcaggtag tcggcacgca attgcaggat cgcctcggct
     1021 cctccctctg accagaactt ctcggttccc ttcacgcggc ggttgatcat cttgattacc
     1081 gactccacag cgctggtcat gattggcagc ccttggcggc gatactcgtc atagtgcatc
     1141 cgctccgcgt tgttcctcag gtaacgcaac gcatcgtaca ccaattgtcg cgggtcggtc
     1201 tcggcgcact gcggtggcgg cgtacccaac gccgccgaac gcgcctccaa ttcgggcaag
     1261 atcgtcgcca cctgaccgga ccagaccgct tgaatccacc gcttgtaaac catctcgcct
     1321 tcgccctgcg ggcggcccgc gaacgccccc gcgaacacgt agctcaacgc atgcacgaag
     1381 tcgagaatcg cggtgaatgt gctgaaatgc tcccgctgga tgctccagtt gactggcagg
     1441 ccgtccccca gaaagacccg ccgcgccgcc ccaaagaagt tccgctccca ggccgcctga
     1501 tgcaccatcg gcccgaagtc gtcgctgcac gcccggctcg ccagaacact ccggaccaga
     1561 cgctccgggc gtcccgggcg ggccgcgcgg tcacggcccg tcgccgttgc ctcatccggt
     1621 tcctcgatcc ggtcttcgac cgcgaactcc aggcccgtcg gcagcgcgtg gccgaggcca
     1681 cgcaccatct cagccgtccg cttcaggtcc aggaaacagc ggggaacgtc cgggtccggg
     1741 tccacgtcgc tgacggcact gaggtacgtc tccaggaccg cgacttttga ttcccgccag
     1801 tggccctttc gtgccggctc cgatgccttc ggcagcgggt ccgacgactc ctgcagcggc
     1861 tccgacgctt gcgacggcag cggcgtccgc acctgcaatc ggcccccatc caccgacacc
     1921 atggccactg ccgggcaggg acgttgtggg tttgcggcgg cgtctttctc catcagaggc
     1981 aaggccgcat gcgccgcgac cgcggtgtcg cgctggtcgg tccgctcgcg gccaatccgg
     2041 tggaccagtc gctcgatcgg cttgggctcg accgccaggt cgcccagtgc ggcgaggtcg
     2101 cgggaggcct gttgatagga aggactgttt actccggcat gcacgatctt ctgctgcacg
     2161 cggggactga agcctgtacc atcaaggccc agcgcctggt tctgagggaa aaaaagcccg
     2221 ccgacaacgg gggcagtttc cgacgcgttc cttccaggcg acatcccctc gcgtagtggt
     2281 caccacgcgg ggctcattgg gcgccttgcg agccgccccg ccgcagagcg ggcattcgcc
     2341 cagttcctcc tcgggccagt cctcggcctg ccctcggatg tggatctcga tgagttgtcg
     2401 ggccatctca tcacccaggg caccggcaac gtcctcgagt tccgagaact tggtgcctcg
     2461 gggaaggcca tcgggataga gctctcgccc caattcgcgg gccatctgac ccaagcggtc
     2521 ctggaattcc ttccgaatcg cggcagcatc cttgcccatc ttggtagtcc tcaccaagtt
     2581 gaagatcgag gagtcgaaga cctgcggaat ccgatcggta ggctaacatc cacccccttt
     2641 gtcgtgcaaa ccatgtcgtg cgcccacgtc gaa
//
LOCUS       DATAQL010000755         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2825759, whole genome shotgun sequence.
ACCESSION   DATAQL010000755 DATAQL010000000
VERSION     DATAQL010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825759"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..364)
                     /locus_tag="VHS97_13165"
     CDS             complement(110..364)
                     /locus_tag="VHS97_13165"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX3449202.1"
                     /translation="MLGETRSLSTISVTKWGGPQTGPVMLAYPAAPGIDALLARAAGE
                     KPAVDVTADDGVCHQLWVIDDEATIAAHARGRCFACALYR"
     gene            764..2491
                     /locus_tag="VHS97_13170"
     CDS             764..2491
                     /locus_tag="VHS97_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HEX3449203.1"
                     /translation="MRTRRWMWCGLAAASALWPAIVAAGEIGNYSPVTADRLTNPEPG
                     NWMLYRRTYDGQGYSPLDKINTTNVKNLVPAWTFSTGVTEGHEAPPIINNGIMFVATP
                     QNQVIALDAKTGDQIWRYKRELPEDLTQLHPTSRGVGLWQDKLYLATTDDHLVALDGK
                     TGKVAWDQKVQDYKKGQYLTLMPLVVNGKVLVGGSGGEYGVRGYVVAYDANDGKELWR
                     TFTIPGPGEPGHETWQGDDWKSGGGSAWMTGNYDPALNLVYWGVGNAAPWPGDLHPGD
                     NLYTTSVLALDPVSGNIKAYHQYHPNDSWDWDEVEAPMLIDLQKDGQTIKSLVHPGRD
                     AIFWVLERSPDKIRFVAGWPFVYTDVWKSIDPETGKPTPDPEHKPALGKKIAFCPSLW
                     GGKDWPSAAYSPKTRLVYVPAQNNFCGSFVGAKESLEPGKLWLGTKEEDILLTERPGS
                     DHYGELQAWDPATGKKAWQHNFRWQLMATVLATGGDLVFVGGTNDRMFRAFDAKTGDQ
                     LWEQATNSGITGAPVAYEVDGTEYIAVQSGWGVDAERIQDKLAKANIGVSDNVPQGGV
                     VWVFKVKQP"
BASE COUNT          501 a          800 c          787 g          442 t
ORIGIN      
        1 catacctttg atccaacgaa gcgcggacct aattcgccgc ttcggcccgc ggtcctgtga
       61 gtttccgtcg agcgccacgc gcaaggccgc ggccgagcgg tgatggccgt cagcgatata
      121 gagcgcaggc aaagcatcga ccgcgcgcgt gcgccgcgat cgtcgcctcg tcgtcgatca
      181 cccagagctg gtggcacacc ccgtcgtcgg cggtgacgtc gaccgctggt ttttcaccgg
      241 cggcgcgcgc gagcagggcg tcgatcccgg gcgccgccgg ataggccagc atgaccgggc
      301 cagtctgcgg accaccccac ttcgtgactg agattgttga gagactcctg gtctcgccca
      361 gcaaacttac ttgaggcctt ccggtcgcag ccgcgttctc gcaatttcaa ctacttacgg
      421 acgtatgctc tggttccgca gtgtgctacg catgtgggct cgatacgatt gaaaatcagg
      481 tccactgccg cggaggacgg cgttaccgat cagctctcta aactcttggc tctcgatagt
      541 gactcggttg tgagaagctt ggttaatatg ccgattggat ttccccccga ggtcagatat
      601 tctgatctcg ccgggctcgt cgctcaacaa aatccaatag aaaccgcgac ttaacctggc
      661 tcggacgagc aggttcattg acaaactagc gacccggtaa atcgaccgat gaggcgttat
      721 cggctccgat gacacactcg gccttcgacg agggaggaag caaatgcgaa cgcgacgatg
      781 gatgtggtgc ggtttggcgg cggcgtcggc tttgtggccg gcaatcgtcg ccgcgggcga
      841 gatcggcaac tacagcccgg tgaccgccga ccggctcacc aaccccgagc ccggcaactg
      901 gatgctctac cgccgcacct atgacggcca gggctacagc ccgctcgaca agatcaacac
      961 cactaacgtc aagaatttgg tgccggcgtg gacgttttcg accggcgtca cggaaggcca
     1021 cgaggcaccg ccaatcatca acaacggcat catgttcgtg gcgacgccgc agaaccaggt
     1081 catcgctctc gacgcgaaga ccggcgacca gatctggcgt tacaagcggg agctgcccga
     1141 ggacctcacc cagctgcacc cgacgagccg aggtgtcggg ctttggcagg acaagcttta
     1201 ccttgccacc accgatgatc acctcgtggc gctcgacggc aagaccggca aggtggcctg
     1261 ggaccagaag gtccaagatt acaagaaggg tcagtacctg acgctgatgc cgcttgtggt
     1321 caacggcaag gttctcgtcg gcggctcggg cggcgaatac ggcgtccgcg gctacgtcgt
     1381 tgcttacgat gccaacgacg gcaaggagct ttggcgcacc ttcacgatcc ccgggccggg
     1441 cgagcccggc cacgagacct ggcagggcga cgactggaag agcggcggcg gctcggcctg
     1501 gatgaccggt aattatgatc ccgccctcaa cctcgtctac tggggtgtcg gcaacgccgc
     1561 gccctggccc ggcgatctgc atccgggcga caacctctac accacctcgg ttctggcgct
     1621 cgatccggtc tcgggaaaca tcaaggccta ccaccagtac cacccgaacg attcttggga
     1681 ttgggacgaa gtcgaagcgc cgatgctgat cgatctgcaa aaagacgggc agacgatcaa
     1741 gagcctggtc caccccggcc gcgacgcgat cttctgggta ttggagcgca gcccggacaa
     1801 gatcaggttc gtcgcgggat ggccctttgt ctacaccgac gtgtggaagt cgatcgaccc
     1861 ggagaccggc aagcccactc ccgatcccga gcacaaaccc gcgctcggta agaagatcgc
     1921 gttctgcccc tcgctgtggg gcgggaagga ctggccgtcg gccgcttaca gcccgaagac
     1981 gcgactggtc tacgtgccgg cgcagaacaa tttctgtggc agctttgtcg gtgccaagga
     2041 gtcgctcgag cccggcaagc tctggctcgg gaccaaagaa gaggacatcc tcctaaccga
     2101 gcgtcccggc tcggatcact acggcgaact tcaagcctgg gatcccgcca ccggcaagaa
     2161 ggcatggcag cataatttcc ggtggcagct gatggccact gtgctggcca ccggtggcga
     2221 cctcgtcttt gtcggcggca ccaacgaccg catgttccgc gctttcgacg ccaaaaccgg
     2281 cgatcagctg tgggagcagg cgaccaattc cggcattacc ggcgctcccg tagcctatga
     2341 ggtcgacggt accgagtaca tcgccgtcca gtccggctgg ggcgtcgacg ccgagcgcat
     2401 ccaggacaag ctggcgaagg ccaatatcgg ggtcagcgac aacgtacccc agggcggcgt
     2461 cgtctgggtc tttaaggtga agcagccgta gcagcggcgg catttggcag cgatctgggc
     2521 aggcctttgg
//
LOCUS       DATAQL010000756         6256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1146689, whole genome shotgun sequence.
ACCESSION   DATAQL010000756 DATAQL010000000
VERSION     DATAQL010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146689"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..594)
                     /locus_tag="VHS97_13175"
     CDS             complement(202..594)
                     /locus_tag="VHS97_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX3449204.1"
                     /translation="MSEKEGREAPGPATLQNPQAQQQTEVVVDDSGTLPSYSNFCRVT
                     ATPEEVILDFGLNPQPFATGRQDVKANQRIVMNFYTAKRLLTALGMTIQRHEGTFGSI
                     ELDVRRRASSPPQTYTGPGRVGPQGVVE"
     gene            complement(738..2051)
                     /locus_tag="VHS97_13180"
     CDS             complement(738..2051)
                     /locus_tag="VHS97_13180"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX3449205.1"
                     /translation="MPFALNVLYSAALVIFSPLLLYRWIRAGKYRDGWSEKFRGEAPL
                     RIGGHPCLWFHAVSVGEVLLLRPLVREMARRRPSWEVVISTTTSTGLAVARKTYPDLV
                     TFYAPLDFSWATRRAVARIRPTVLALVELELWPNLIRAAKRTGAKVAIINARLSSRSY
                     RGYRRFRTPLGPTLGCIDVVAAQDEEYARRFVELGIPSHRVSVTGSVKYDGLESDRNN
                     IKTLELRQLLGLAATDLIFVAGSTMEGEEAAVLAAYKAARRQHSSLRLILVPRHAERF
                     EAVARWLEQHGETVMRRSRVKSPIPRGLGNPSPIILIDSIGELGAIWGLADVAFVGGS
                     LRPGRGGQNMMEPAAYGASVIFGPHIANFRETVDQLLERKAARQVADAGELTLGLLAD
                     LDDPEAAAARGAAGRDFVLAQNGAAGRTLAQLDRLVESSLVKRTA"
     gene            complement(2380..6006)
                     /gene="secA"
                     /locus_tag="VHS97_13185"
     CDS             complement(2380..6006)
                     /gene="secA"
                     /locus_tag="VHS97_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX3449206.1"
                     /translation="MELLSDIWDKTTDVFTAFSENVSEGLVRLFGSSNERRIRHMRAL
                     VAQINELEPGMTALSAEELRAKTDELRARRAAGESLDDLLPEAFAAARESGRRYLNMR
                     HFDVQLMGGIVLHGGNIAEMVTGEGKTLVATLAAYLNALDGRGVHVVTVNDYLARRDA
                     EWMSPLFRGLGMTVGAIQSEMDSAERQEMYSCDITYGTNHELGFDYLRDNMKPTKELQ
                     SQKELFYAIIDEVDSILIDEARTPLIISGPAFDDVRKYAEADRIARLLRKDLHFEIKE
                     KERTCHLNDEGVREAEKIAGLESFYTPGNMEWPHLIDNSLKAHHLYKRDRDYVVQPDG
                     EVVIVDEFTGRLMIGRQWSDGLHQAVEAKEHVRIKEENQTLATITLQNFFKLYKKLAG
                     MTGTAMTEATEFYKVYGLDVIAIPTNRSLVRFNHSDVIFRYEREKLQAILDEIKEVNA
                     SGRPILVGTTSIEKSEELAEMLKRFGIPHQVLNAKYHEREAEIVAQAGRHEAVTIATN
                     MAGRGTDIILGGNPEFMAWADLKRMTDEDGRPLYPTRLEVPKDVWEQAVARYEPEMRV
                     EGREVAAVGGLHIIGTERHESRRIDNQLRGRAGRQGDPGSSRFFLSLEDDLMRKFAGE
                     WVSAVLTRLGMQEGEAIESKMVSRRIDGAQKKVEERNFDIRKNLLEYDEVMDEQRKRV
                     YSFRQSLLEGAPPKETLNEMIDNQIDEAAKRFLADDYGAASFAESVGQRLGIELTARE
                     IKGASFEDAEEIAKTKAERQLRESIRQAMDENLPSDADPSEWTWQALANWANARFDLN
                     LKDRDLKKHASSSNDEFQFGHADLEEFLNELAVQSLAKIDLKEAREFLLPDWGRRSFS
                     GWVHHKFATIIDPASWADLDRAEIVRRVRTIAREIYAQKESELPTRIALVKYLGDRSQ
                     SQNPRYDREGLAAWASSRYHTDIKEEELRPLLRPEIEERLISLAHQHYQGATLSQELE
                     AKLAAIKPTGSHPDKNGTPADPQALVDLAAWARRSLQVELTDDNLHSMSTAEIRRTLL
                     NALDAKERPEMREMEKAVLLQILDSSWMEHLRAMDHLRSSIGLQGYAQIDPKVEYKRE
                     GMRIFAEMWNGIGDRVTDLIFRVEQFDPEFLTYLGSQWKLERAQTIHHQADSQLAPSA
                     DRNGVRNQQDAAIAGSQRSPEKKREPVRNLAKKVGRNDPCPCGSGKKFKACHMRKQSM
                     GDPF"
BASE COUNT         1156 a         1859 c         1908 g         1333 t
ORIGIN      
        1 ctcttcctga gccgtagacg gcgctctaac gaccgtcgaa ttgcgaccgg gccatccatg
       61 cggtcgaggc ttggcagctg gctggtctcg cgatgcgcct gcatcgcgag accgcatggg
      121 actgacattg aaaggagttg cccaccccgc ggacatcggg tgcggacgac gcaatccgcg
      181 tcgtctgtgg gcgtaacgtg cttactcgac gacgccttgg ggacctacgc ggcctgggcc
      241 cgtgtaggtc tggggaggac tggaggcgcg gcgcctcacg tcgagctcaa tcgatccaaa
      301 ggttccttcg tgccgctgaa tcgtcatgcc aagagcggtc aggagtcgtt tggccgtgta
      361 aaagttcatc acgatccgct ggtttgcctt gacgtcctgg cggcccgtgg cgaacggctg
      421 cggattaagg ccaaaatcga ggatgacttc ctcgggggtc gctgtgactc ggcagaaatt
      481 ggagtacgac ggcaacgtcc ccgaatcgtc caccacgacc tcggtttgct gctgcgcctg
      541 gggattttgc aaggttgccg gtcccggcgc ttctcttcct tccttctcgc tcatctcatt
      601 ctcccagttc tgagcaacgg catgttaagg cgagccgttc agatctttga tacatgaaga
      661 tcggaagttg ctgccttcgc gcgcagccga tttggatcct atttcagcga agcccatgtg
      721 aaaacgcaag ccggagctca cgcggtccgt ttgacaagtg aagattcgac aagcctgtcg
      781 agctgggcga gggtgcgtcc ggcggccccg ttttgtgcca gtacaaagtc tctcccggcg
      841 gccccgcgcg cggccgcggc ctccggatcg tcgagatcgg cgagcagacc gagggtcaac
      901 tcgccagcgt cggcaacctg ccgcgccgcc ttgcgctcga gcaactgatc cacggtctcg
      961 cgaaaattgg cgatgtgcgg cccgaaaata acggatgcac cgtacgcggc cggctccatc
     1021 atgttctggc cgccacgccc ggggcgcagg cttcccccca cgaatgccac gtcggccagt
     1081 ccccagatcg ctcccagttc gccgatgctg tcgatcagga taatcggcga cggattgccc
     1141 aagcctctag gaatcggcga cttcaccctg ctgcggcgca tcacagtctc gccgtgttgc
     1201 tcgagccagc gcgccacggc ctcgaaccgt tcggcgtggc gagggacgag gatcagtcgc
     1261 aagctcgaat gctgccgccg ggccgccttg tacgcagcca ggaccgcggc ttcttctccc
     1321 tccatcgtgc tccccgccac gaaaatcagg tcggttgcgg ccaggccgag caactgcctt
     1381 agctcgagtg tttttatgtt gttgcgatca ctctccagcc cgtcgtattt gaccgagcca
     1441 gtgacgctaa cgcggtggct aggaatgccg agctcgacaa atcgacgcgc gtattcctca
     1501 tcctgcgcag ccaccacatc aatgcaaccg agagtgggcc ccagcggagt acgaaatctg
     1561 cggtatccgc ggtaactgcg cgaactgagg cgggcattga taatcgcaac cttggcaccg
     1621 gtgcgtttgg cggctcggat caggttgggc cagagctcga gctcgaccaa ggcgagtacg
     1681 gtgggacgaa tgcgtgcgac ggcgcgccgc gtggcccagc taaagtcgag cggagcgtag
     1741 aaggtgacca ggtcgggata ggtcttgcgg gcgacagcca gcccggtcga agtagtcgtc
     1801 gatatcacga cttcccagct cggacgacgg cgggccattt cgcgaaccaa gggacgtaag
     1861 agcaagacct cgcccacgct cacggcgtga aaccagagac aggggtggcc gccaattcgt
     1921 agaggcgcct cacctcgaaa cttttcactc caaccatcgc gatacttgcc cgcacgtatc
     1981 catcggtaca agagtaaagg cgaaaaaatg accagcgcgg cggagtaaag cacgttgagg
     2041 gcgaatggca tctcggggaa tcctttcccg gaacgttgcg gcgcctttga caccgcgtga
     2101 ctcgcaccat ggtagcaact gtcgtgcctc aaacgtggtt gccgaaaagc ctggctttct
     2161 ccgaggccaa cagacgacga tttccagcga aaaaaggcca atcgcggccg tgcgcgccca
     2221 actctcgacc ttacctggtc gctgcaccta agtctcggtc accatgacag atcatgtcca
     2281 gacgacgata tcgcggcgcc cgatttctcg gcgtatcaat caccgatcga tgggcggaga
     2341 tgagaagttt tgcaaaacgg aacaaactaa gtcgattggc tagaatgggt cgcccatcga
     2401 ttgtttgcgc atgtggcaag ctttgaattt tttaccggaa ccgcaggggc aagggtcgtt
     2461 gcgaccgacc ttttttgcaa ggttgcggac aggttcgcgt tttttctcgg gggaccgctg
     2521 gctgccggcg atggccgcat cttgctgatt gcgaacgccg ttcctgtccg cgctgggggc
     2581 gagctgagag tcggcctgat gatggattgt ctgggcgcgt tcaagcttcc attgagagcc
     2641 caagtaagta aggaattcgg gatcgaactg ttcgactcga aagatcaggt cggtgacacg
     2701 gtcgccgatg ccgttccaca tctcggcgaa gatcctcatc ccttcgcgtt tatactcgac
     2761 cttgggatcg atctgggcgt aaccttgcaa accgatggag ctgcggaggt gatccatggc
     2821 gcgtaggtgt tccatccagc tcgagtcgag aatctggagg aggaccgcct tttccatctc
     2881 gcgcatctcg ggccgctcct tggcgtcgag agcgttcaag agggtgcggc gaatctccgc
     2941 cgtcgacatg gaatggaggt tgtcgtcggt caactccact tgtaaggacc gacgggccca
     3001 cgcagccaga tcgacgaggg cctgaggatc ggcgggagtg ccgttcttgt cgggatggga
     3061 accggttggc ttgatcgccg cgagcttggc ttcgagctct tgggacagcg tggcaccctg
     3121 ataatgttgg tgagccaggg agatcaagcg ttcctcgatt tcgggtcgaa gcaaggggcg
     3181 aagctcttcc tccttgatgt cggtgtggta gcggctggac gcccaggcgg cgagaccttc
     3241 gcggtcgtag cggggatttt ggctctgcga gcggtcaccc aagtatttga ccagagcgat
     3301 ccgcgtgggc agctcggatt ccttctgggc atagatctca cgtgctatgg tccgaacacg
     3361 gcggacaatc tcggcccgat cgagatcggc ccagcttgcc ggatcgatga tcgtggcgaa
     3421 cttatgatgg acccagccgg agaaggaacg gcgtccccag tccggcaaga gaaactccct
     3481 tgcttccttg aggtcgattt ttgccagcga ttgaactgcc agttcgttca agaattcctc
     3541 gaggtcggcg tggccaaact ggaattcatc gttactggag ctcgcgtgtt ttttcaggtc
     3601 tctgtctttg agattgaggt cgaagcgcgc gttggcccaa tttgccagag cttgccaggt
     3661 ccattcggat ggatcggcat cgcttgggag gttttcgtcc attgcctggc ggatgctctc
     3721 gcgaagctgc cgctcggcct tggtcttggc gatttcctcg gcgtcttcga acgaagcacc
     3781 cttgatttcc cgcgcggtga gctcgatgcc cagacgctgg ccgaccgact cggcgaagct
     3841 ggccgcgcca taatcatcgg ccaggaatcg cttggcggct tcatcgatct ggttatcgat
     3901 catctcattg agcgtttcct tgggaggagc cccctcgagc aggctctgcc ggaaggaata
     3961 aacccgcttg cgctgttcgt ccatgacttc gtcatattcg agcaggtttt tgcggatgtc
     4021 gaagttgcgt tcctcgacct ttttttgtgc tccatcgatc cggcggctga ccatcttgct
     4081 ctcgatggct tcgccctcct gcattccgag ccgggtgagg acggcggaga cccactcgcc
     4141 ggcgaacttg cgcatcaggt cgtcttccag cgagaggaag aagcgcgatg agcccgggtc
     4201 tccctggcga ccggcgcggc cacgtaattg attgtcgatt cgtcggctct cgtgccgctc
     4261 ggtgccgatg atgtgcagac cgccgaccgc ggcgacctcg cgcccttcga ccctcatctc
     4321 gggctcgtaa cgagcgacgg cctgttccca gacatccttg gggacttcga gccgcgtggg
     4381 gtagagcggg cggccgtctt cgtcggtcat cctcttcaag tcggcccagg ccatgaactc
     4441 cgggttgccc cccaagataa tgtcggtgcc gcggccggcc atgttggtcg cgatcgtgac
     4501 cgcctcgtgc cggcccgcct gagccacgat ctcggcctcg cgctcgtggt acttggcatt
     4561 caacacctgg tggggaattc caaagcgttt gagcatctct gccagctctt ccgacttttc
     4621 gatcgacgtt gtgccgacca gaatggggcg gccactggcg ttgacctctt tgatttcgtc
     4681 aaggatcgcc tgcaacttct cgcgctcgta ccggaagatt acgtcggaat ggttgaaacg
     4741 cacgagcgag cggtttgtcg ggatggcgat gacatcaagc ccgtaaacct tgtaaaactc
     4801 cgtcgcctcg gtcatggcgg tgccggtcat cccggcgagt tttttgtaga gcttgaagaa
     4861 gttttgcagg gtgatggtcg cgagcgtctg gttttcttcc ttgatccgga cgtgctcctt
     4921 ggcctcgacg gcctgatgca aaccatcgga ccactggcgg ccgatcatga gccggccggt
     4981 gaattcgtcg acgatcacga cctcgccgtc gggctggacg acgtaatcgc gatcgcgctt
     5041 gtaaaggtgg tgggccttga gcgagttgtc gatcaggtgg ggccattcca tgttgccggg
     5101 cgtgtaaaag ctctcgaggc cggcgatctt ctcggcctcg cgcacccctt catcgttcaa
     5161 atgacaggtc cgctcctttt ccttgatctc aaaatggaga tctttgcgca agagacgggc
     5221 aatccgatcg gcctcggcgt acttgcgcac atcgtcgaag gcgggtcccg aaatgatcag
     5281 cggtgtccgg gcctcgtcga tcagaatgct gtcgacttcg tcgatgatgg cgtaaaagag
     5341 ctctttttga gattggagct ctttggtcgg cttcatgttg tcgcgcaggt agtcgaagcc
     5401 caactcgtga ttggtgccgt atgtgatgtc gcacgagtac atctcctgac gttcggcgga
     5461 gtccatctcc gactggatgg cgccgacggt catgcccagt ccgcgaaaga gcgggctcat
     5521 ccattccgcg tcgcgccggg cgaggtagtc gttaaccgtc acgacatgga cgccccggcc
     5581 gtcaagggcg ttcaaatagg ccgccagggt ggcgaccaga gtcttgcctt cacccgtgac
     5641 catctcggcg atgttgccgc cgtgcagcac gatcccgccc atcaactgga cgtcgaagtg
     5701 tcgcatgttc aagtagcggc gccccgactc acgggcggcc gcgaatgcct caggcaagag
     5761 atcgtcgagc gattccccgg cggcccgccg ggctcgcaac tcgtcggtct tggcgcgaag
     5821 ctcctcggct gacagcgccg tcatacctgg ctctagttca ttaatctggg cgactagcgc
     5881 ccgcatgtga cggatccgac gttcgttgga gctgccgaac agacggacca gtccctccga
     5941 aacgttttcc gagaaggcgg tgaaaacgtc ggtcgtctta tcccaaatgt cgctcagcag
     6001 ttccattgcc caagaggccc cacgaaaagg taaagaatca gcgcactaag tcttttaaaa
     6061 atgccatctt acatcggatc gccgcgaggc gtcaagccgg ccggacggcc aacgcccgcg
     6121 cttctggttc tggtaactaa agaacggacg gtcgctggcg cattcctcgc ttgaggctgg
     6181 cttcattgag ttttgcttgc tttacagacc aatctccggt catggcacaa accgcactcg
     6241 atggcaacac gccacc
//
LOCUS       DATAQL010000757         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1299020, whole genome shotgun sequence.
ACCESSION   DATAQL010000757 DATAQL010000000
VERSION     DATAQL010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299020"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..709)
                     /locus_tag="VHS97_13190"
     CDS             complement(371..709)
                     /locus_tag="VHS97_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449207.1"
                     /translation="MKTNWKSLALVGGLALGCFVVGNTPARAQGFSFGYAGPGVSVGV
                     NTGGGFYGGGFYGGGYPVVTPAPVVVAPYAPVVVPRPVYVARPFYGPRPFYGPRVYGG
                     YRPYPNRYWR"
     gene            1046..2278
                     /locus_tag="VHS97_13195"
     CDS             1046..2278
                     /locus_tag="VHS97_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3449208.1"
                     /translation="MAHEETDLTDRRKFLQAGALATASAVSLSSSVPAQELVAKSPLL
                     PKRKLGKTGVEISILEAGAVRSDERVLRIAYANGIRIFDTAKVYGTERNFKKWFEQDA
                     KVRKEIFLVTKDMPRVPKDMLKMVDERLETLGTDYIDLFFIHGLGDQRTVDESINMVA
                     SQEFKETAEALKKSGKIKFIGFSSHNKDRAPIISAAAKAGYIDAIMIQYRPWLEKDSP
                     LNKALDLCWKNEIGVISMKQIAGNGFGDKPKGDILKDVVAKVPMLKERKLSPFQGLLH
                     AIWTDERISAACVSMKNTDHVRENADAARRFEPLKQADIHQLRDVFLASNPTMCADCD
                     GRCSVAAGTQAELGDLTRFLTYHQQYGDRAKARREYAALAPEARDWSGADLEAAQAAC
                     PGRLNFAKLLPEVERHLA"
     gene            2370..2795
                     /gene="arfB"
                     /locus_tag="VHS97_13200"
     CDS             2370..2795
                     /gene="arfB"
                     /locus_tag="VHS97_13200"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX3449209.1"
                     /translation="MIRINPRISIDESEIELEFIRASGPGGQNVNKVSTAVRLRFDVR
                     HSPSLPPDVRDRLIRLAGKKVGDDGFLMIHARRCRTQESNRRDAIERLVDLIEKACER
                     PTPRRATKPTAGSQKRRLDAKKHRGAKKESRRVRDPSDD"
     gene            2809..>3134
                     /locus_tag="VHS97_13205"
     CDS             2809..>3134
                     /locus_tag="VHS97_13205"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3449210.1"
                     /translation="MTPRLDNARALGRARVVLLTLVTLALAPAYLLADERSLAAAKES
                     LVGFDSWTNEAIHVWEVPGLAIAVVKNGEVILAEGYGLRDVDKKLPVTPKTLFAIGSC
                     TKAFTT"
BASE COUNT          672 a          958 c          955 g          549 t
ORIGIN      
        1 agaatacgag cggttggtgg aggccgacgt gctcgacgat ccccgcgtcg agcggtcaag
       61 atgtgccagt agtcatcgaa gacgctctcg ccggccggat cgccgtatcc gacattcttc
      121 cgcaaaaaag taatcgaagt cgaacgacct ggcagggggc aacgacctgc gcaagataga
      181 cctcgcgggg gcggattcga tccattttcg ggaagggcca ggctccgccc gagccgtcgc
      241 ggattgggaa gcggctcggc gggagcctcg ccctcccgct ccaaaaagtc gcgtttgccc
      301 ccgcgcgagg tatagctctc ctgggagcct cgcattcccg aatgcgtatc ccgatcgcgg
      361 ctgaactcgc tcaccgccaa tagcggttag ggtaagggcg gtatcccccg taaacccttg
      421 gcccataaaa cggccgcggc ccataaaatg gtcgcgcgac atacacgggc cggggcacga
      481 ccaccggggc ataaggcgcg accacgacgg gggcgggcgt gacgacagga tagccaccgc
      541 cgtagaaacc gccaccgtag aagccgccgc cggtgttcac accgacagag actcccgggc
      601 ccgcatatcc gaacgaaaaa ccttgggcgc gtgccggcgt atttccgacg acgaaacagc
      661 ccagagccaa gccacctact aacgccaacg acttccaatt cgttttcatg gctggtgttc
      721 ccttagagag ggttctcggg ttcttgcccc gactcaggtg caatgcggag accaacaccc
      781 atctaatcgt cggcgaccgt cgaaaggttc gaatcgccca cgtcgaccgc tctctattct
      841 gagtatccac agtcaaatat ccggcgtcaa ccgaaagctg taagcagaga ccgttgatgc
      901 gtacgcctgc gacgggtaaa gtaatgtgtc gttcgccgcg tcgcagccag gataagccag
      961 ttgagggaca cgggtcgcct ggctcaactg tgagcggttt ctgagaaaaa gatgcgatca
     1021 cccgcctgac caggagtcaa gagcaatggc ccacgaagaa acggacctta ccgaccgccg
     1081 taaattcctt caggccggcg cgctggcgac ggcgtcagcg gtgagtttgt cgtccagcgt
     1141 gccggcacag gagctggttg cgaagtcccc gctcttaccc aagcggaaac tgggcaagac
     1201 aggagtggag atctcgattc tcgaggcggg cgcggtgcgc agcgacgagc gcgtcctccg
     1261 gatcgcctac gccaacggca tccgtatttt tgacacggcc aaggtctacg ggacagagcg
     1321 caatttcaaa aagtggttcg aacaagatgc caaggtccgt aaggaaatct tcctcgtcac
     1381 caaagacatg ccgcgcgtgc ccaaagacat gctcaagatg gtcgacgagc ggctggagac
     1441 gttggggacc gactacatcg acctcttctt catccacggg ctgggcgatc agcgcaccgt
     1501 cgacgaatcg atcaacatgg tcgcgagcca ggaattcaag gaaaccgccg aagccctcaa
     1561 aaagtcgggc aagatcaagt tcatcggctt ctcgagccac aacaaagatc gtgcgccgat
     1621 catttcggcg gcggccaagg cgggctacat cgacgcgatc atgatccagt atcggccctg
     1681 gctggaaaaa gactctccgc tcaacaaggc gcttgacctt tgctggaaaa acgagattgg
     1741 cgtgatctcg atgaagcaaa tcgcgggcaa cggctttgga gataagccca agggagacat
     1801 tctcaaggac gtcgtcgcca aggtaccgat gctcaaggaa cgtaagctgt cgccgttcca
     1861 gggtctcttg catgcgatct ggaccgatga gcggatcagc gccgcctgcg tttcgatgaa
     1921 aaacaccgac cacgtgcgtg aaaatgccga cgccgcccgc agattcgagc cgctcaaaca
     1981 ggccgatatc caccagcttc gtgacgtttt tctcgcgtcg aacccgacca tgtgcgcgga
     2041 ttgcgacggc cgctgctcgg tcgccgcggg cacccaggcc gagctcgggg acctgacccg
     2101 gttcctgacc tatcaccagc agtacggtga ccgtgccaag gctcgtcgcg aatacgccgc
     2161 gctcgcgccc gaggcccgcg actggtccgg agccgacctc gaagccgccc aggcggcgtg
     2221 cccgggccgg ctcaacttcg ccaagctctt gcccgaggtc gaacggcatc tggcttgaaa
     2281 agcagtcctt agtcattggt ccttggtcct tggtccttgg cccttaagta acgagccgtt
     2341 gtagatgggt tttgctcgat cgcggtttca tgatacgaat taatccacgt atctcgattg
     2401 atgaaagcga gatcgagctc gaattcattc gcgcgtcggg gccgggcggc cagaacgtca
     2461 ataaggtgtc gacggccgtc cggctgcgat tcgatgtccg tcactcgcca tcgctgccgc
     2521 cggacgtgcg cgaccgcttg attcgcctgg ccggcaaaaa ggtcggtgac gacggcttct
     2581 tgatgatcca tgcccggcgg tgccggacgc aggagtccaa ccgccgcgac gccatcgagc
     2641 ggctggtcga cctgattgaa aaagcgtgcg agcggcccac gccacggcgg gcgaccaagc
     2701 ctacggccgg ttcgcagaaa cgccggctgg atgccaagaa gcatcgtggt gccaagaagg
     2761 aaagcaggcg ggtgcgagac cccagcgatg attagcggga gacagttcat gacgccgaga
     2821 ctggacaacg cccgcgcctt gggccgagcg cgcgtggtgc ttttgactct cgtgaccctc
     2881 gcacttgctc cggcctacct cctcgcggac gagcgctcgc tcgcggccgc caaggagtcg
     2941 ctggttggat ttgactcgtg gaccaacgag gcgatccacg tgtgggaggt gccagggctg
     3001 gcgattgcgg tcgtcaagaa cggtgaggtg attctggcag aaggctatgg ccttcgcgac
     3061 gtggacaaga agctgccggt cacgcccaaa acactcttcg ccatcggttc atgcaccaag
     3121 gccttcacga cgtt
//
LOCUS       DATAQL010000758         4040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3435427, whole genome shotgun sequence.
ACCESSION   DATAQL010000758 DATAQL010000000
VERSION     DATAQL010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3435427"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VHS97_13210"
     CDS             <1..414
                     /locus_tag="VHS97_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449211.1"
                     /translation="SLARRAQWLRGHSQLGEVLWDRPIPWEGWSLTRAGKIAIVTAAD
                     GRALTCDGSGTFRAQSAPSGDSNDVFGIDPVGEPIRISRRGVHIIAAALDGRVRWRSV
                     VELPLGPLAAGTSGVAIMLGKSLAWFPHDQAGATT"
     gene            791..1222
                     /locus_tag="VHS97_13215"
     CDS             791..1222
                     /locus_tag="VHS97_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449212.1"
                     /translation="MEMSTESRMSLHRCGEEEAQRYKWIESEKAGRDLGETAIRRWIG
                     EHWHGFLRHRWLEHLQGKTFWIELDQGDFGLLRTAFQASPLIDPIVEHFKRGGENLDI
                     ILWALDNELPLNDVVEILEALDVNSRRIECRFDPRRFKMLT"
     gene            complement(1247..2755)
                     /locus_tag="VHS97_13220"
     CDS             complement(1247..2755)
                     /locus_tag="VHS97_13220"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX3449213.1"
                     /translation="MKIALLRQPIESIQAQWLVLGIFEDDAETSQVGRGTPLDARLTR
                     LVNEKELTGSLGELTVFYDVPALEAGAVLVVGLGPKARFEPGAAFSAGFALAKRLAAK
                     RRESVAVVLPPCDDPALIASALIEGSIAGTRGPGVRKSEACRHAFEKLSLVVGNGRTV
                     EELGKLEQSLFRAEIVGNAVNLARDLVNTPPAEKSPKQLANRIGVVAGDAGLDIDIWD
                     ESRIRHERFGGLIGVAAGSDEPPRFVVLEYRRGGHSSTACLVGKGVTFDSGGLSLKPS
                     ASMEDMKSDMTGAAVVVATMQAAARLKLPVNVTGYLALTENMTGGKAMKLGDVLTIRN
                     GKTVEVMNTDAEGRLILADALSYAVEHKPDRVLDLATLTGACMVALGPKVAGLFTNDD
                     AFRHDVTAACRRTGERVWPMPLDDDYKEQLKSHVADLKNVGGKWGGAITAAKFLEQFV
                     GSTPWVHLDIAGPSWNDSDSATRDTGGTGCFVRTLVAYLEGMATPTGHAGTH"
     gene            complement(3111..>4040)
                     /locus_tag="VHS97_13225"
     CDS             complement(3111..>4040)
                     /locus_tag="VHS97_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEX3449214.1"
                     /translation="DMTGIFGWVLALLLNPPQEAEMSERLAPPGWQGNYWGPEATKAR
                     HDGKMPPIVVTQHMARWDRWGRQVLRDGDIVFRLGDARVGRGYFPMSRFLARASNSKF
                     SHTGIVAIEEEGPVVYDISRLSVARQPFCVWILDNIGSIGVKRLRPEFKDAIPQVLGY
                     CHRVFEEQIPFDYELSPNDTALYCVEMTEKAFRSAGIELSRPIRLGDMERASDFPLQM
                     FGIQFASKYVLDHPLTFDHLVYFPGNERHGIWSAAQLMTIVPPTFAPGHPDYIGRVPA
                     ARTPREASSAPSQKVSAPKRQPSGAAAHSGPST"
BASE COUNT          799 a         1209 c         1220 g          812 t
ORIGIN      
        1 agcctcgctc gccgtgccca atggctacgc ggtcacagtc agcttggcga ggtattgtgg
       61 gatcgtccga tcccctggga aggctggtca ctgactcggg cgggaaagat cgcgatcgta
      121 acagccgccg atggccgcgc attgacttgt gatggatcgg gaactttccg cgcacagtcc
      181 gcccccagcg gcgactctaa cgacgtgttc ggcatcgatc cggtgggcga gccgattcgc
      241 atctcccggc gtggcgttca catcatcgcc gccgccttgg acgggcgcgt tcgctggcgc
      301 tccgtcgtcg aactacccct cggccccctg gctgccggca catccggagt cgccatcatg
      361 ctgggtaagt cgctcgcgtg gttcccccac gatcaggccg gcgcaacaac ttgacaccaa
      421 gttgccccat tccccgatga ttccgagcgg caacctaatt tctcaaaacc cacaatttag
      481 ctattctcgc cgcccgcaat caaccattca ttacatgatg tgctacaatc gtcttgtcgg
      541 attggaggac gtgagctaca ctattggaat taccgataaa cgaatcagta tcactggcgg
      601 tatgggaatt tggagttgca ccaacgccac gacgagcgtg ttcgtatagg tcattgggct
      661 caaaagtcaa atgaccgcgt cggaacgcct gactcgaaag tctgagattc ttttgcgacg
      721 aaaccaagga gaggtgccgc atctcagctt ggaggtctcg atgcttgagt gtagtacatc
      781 gatcgctatc atggaaatgt ccactgaatc acggatgagt cttcatcgct gcggggagga
      841 agaagctcaa cgttataagt ggatcgaaag tgagaaggcg ggacgcgatc tcggtgagac
      901 cgctattcgc cggtggatcg gcgaacattg gcatggcttt ttgagacacc gttggctcga
      961 gcatcttcag ggcaagacgt tttggatcga gctcgatcag ggtgactttg gactgttgcg
     1021 aacagccttt caggcatctc cactgatcga tccgatcgtc gagcacttca agcggggggg
     1081 tgaaaacctc gacatcattc tctgggcgct tgataacgag ttgcccttga acgacgtcgt
     1141 ggagatcctc gaggctctcg acgtgaatag ccgccggatc gaatgccgat tcgatccgcg
     1201 gaggttcaag atgttgacct gatcgcggat tgcgatcgga cggcggtcag tgcgttccgg
     1261 catgcccggt cggtgttgcc attccttcca gataggcaac caaagtcctc acaaaacagc
     1321 cggttcctcc cgtgtcgcgc gtcgcgctgt cggaatcgtt ccagctcggc ccggcgatat
     1381 cgaggtgaac ccagggtgtc gagccgacga actgctcgag aaacttggcc gccgtgatcg
     1441 ctccgcccca cttccctccc acatttttca agtcagcgac gtggctcttg agctgctctt
     1501 tgtaatcgtc atcgagcggc attggccaca cgcgctcacc ggtgcgcctg catgcggcag
     1561 tcacgtcgtg ccgaaacgca tcgtcattcg tgaagagacc ggcaactttg ggtcccaagg
     1621 ccaccataca cgcccccgtc agggtcgcca ggtcgaggac acgatccggt ttgtgctcga
     1681 cggcatagct gagggcgtct gccaggatca agcggccctc agcgtcggtg ttcatgacct
     1741 cgacggtctt gccattgcgg atcgtcaaga catcacccag cttcatcgcc ttgccgccgg
     1801 tcatgttttc ggtgagggcg agatatccag tgacattgac cggcaacttc aagcgcgctg
     1861 ccgcttgcat cgtggcgacc acgacggcag caccggtcat atcgcttttc atgtcttcca
     1921 tcgacgcgct cggtttcaag ctcaggcccc cggagtcgaa tgtcactccc ttaccgacca
     1981 ggcacgccgt ggatgaatgg cctccgcgcc ggtattcgag aacgacgaat cgcggtggct
     2041 cgtcggagcc cgcggccact ccaatcaggc cgccgaatcg ctcgtgacgg atgcgcgact
     2101 cgtcccagat gtcgatgtcg agcccggcgt cgccggcgac aacaccgatc cgattcgcga
     2161 gctgttttgg cgacttttcc gccggggggg tattgaccaa gtcgcgtgcc agattgacgg
     2221 cgttgccgac gatctcggcg cggaaaaggc tttgttcgag cttgccaagc tcttccacgg
     2281 tccggccgtt gccgacgacc aggcttagtt tttcgaaggc gtgtcgacaa gcctcgcttt
     2341 tccgcacgcc aggcccgcgg gtgccggcaa tcgaaccttc gatcaaggcc gatgcgatca
     2401 gggcgggatc atcacaagga ggcaagacga cggccaccga ctctcgccgt ttggccgcca
     2461 gccgtttggc cagggcgaaa cccgccgaaa acgccgcacc cggctcgaac cgcgcttttg
     2521 gacccaaccc cactacaagg accgcgccgg cttcgagtgc ggggacatcg taaaataccg
     2581 tgagttcgcc tagtgagccg gtcagctcct tttcgttcac gaggcgcgtg agtctggcat
     2641 ccaggggggt cccgcgcccg acctgcgaag tctcggcatc atcctcgaaa attcccaata
     2701 ccagccactg agcctggatc gattcgatcg gctggcggag gagtgcgatt ttcatcgagg
     2761 tcgggatttt ccaaaaagga taggttatcc gagcgcagtc gaccaatgcg cctggactga
     2821 gccaagctcg ccctgggaag cgattggatc ggtgtctgta gcggacgatc ttgtcatgat
     2881 cgcaggatcg ctccggcggc gccaagccga cttggcgcgc acttttaccg ccaaaccaag
     2941 ggcgctttgg gacgggccgg cgcgagtcgc ggggcggatc gtccgcgggg taagcggtaa
     3001 gggtggttag ccgacggccg tgaccgccgc gcggccgggc tgcgaatccc aggcagcaat
     3061 cgcctaaggc gagtggatcc gggccggatt gccgcactgc tcgtaagggt tcaggtcgac
     3121 gggcccgaat gtgcggccgc accgctcggc tggcgtttgg gtgctgacac cttctgtgac
     3181 ggtgcggacg aggcttcgcg cggcgtccga gccgctggca ctcgccctat atagtcagga
     3241 tgtcccggcg caaatgtggg gggcacgatg gtcatgagtt gcgcggccga ccagattccg
     3301 tggcgctcat ttccaggaaa atacacaaga tggtcaaatg tcaggggatg gtccaaaaca
     3361 tatttggagg caaactggat accgaacatc tggaggggaa aatccgaggc gcgttccata
     3421 tccccaagac ggattggccg agacagctcg atgccggcgg agcgaaatgc cttttcggtc
     3481 atctcgacgc aatagagcgc tgtgtcgttc ggcgacaact cgtagtcaaa cggaatctgc
     3541 tcttcgaaaa cccggtgaca atagccgagg acctggggga tcgcgtcttt gaattcggga
     3601 cgaagccgct tcacgccgat cgaaccaatg ttgtcaagga tccaaacgca aaacggctgg
     3661 cgggccacgc ttaatcggct gatgtcgtag acgacgggac cctcttcctc gatggcgacg
     3721 atcccggtgt gggaaaactt gctgttgcta gcccgcgcca ggaaacggct catcggaaag
     3781 tagccacgtc cgacccgtgc gtcgcccagc cgaaaaacga tatcaccgtc acgaagaact
     3841 tgacggcccc agcggtccca gcgggccatg tgttgcgtga ccacgatcgg cggcatcttg
     3901 ccgtcgtggc gagctttggt ggcttccggc ccccagtaat ttccctgcca gcccggaggg
     3961 gccagccgct cgctcatctc ggcttcttgc ggtggattga gcagaagggc aaggacccag
     4021 ccgaaaattc cagtcatatc
//
LOCUS       DATAQL010000759         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_689661, whole genome shotgun sequence.
ACCESSION   DATAQL010000759 DATAQL010000000
VERSION     DATAQL010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689661"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VHS97_13230"
     CDS             complement(<1..665)
                     /locus_tag="VHS97_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3449215.1"
                     /translation="MAADNGRARNPLVAKLSQFAPLSDQDAALLETLCLPEERFRAGA
                     NIVVEGETPRSAFVLTRGMACRFRLMPDGRRQILTILIPGDFFDLHGFLLKVMDHSVA
                     AIGPTRIAAIGREVVIDIIANHPRIGAALWWSAMQEEAMLRERIVALGRRSARGRVAY
                     ILCELVWRQRAIGMAEDHAIRLPFTQTDLADMLGLTSVHTNRVLQGFRRDELITLAHR
                     RLTL"
     gene            1090..>2222
                     /locus_tag="VHS97_13235"
     CDS             1090..>2222
                     /locus_tag="VHS97_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3449216.1"
                     /translation="MNSSSLDYWQSAAAVVESSNDAIICTDLSGTITSWNKAAKTIFG
                     YRPEEVIGQPVFLLAAPGREDEMPVILDQIIRGEKIQHYETVRCHKEGRQVAISLSVS
                     PIREESGRIVGASKIARDITERTEAEAELRRLNKTLEQRVAERTAELEEVNRQLQAKI
                     AEKERTDRRLRELQSEVFHAARLSAVGQMAATLAHELNQVMTAATNFVNASRRLLSSG
                     DRHKIETVPKVMDEAAAEILRAGRIIGRLRAFVSRGETERRVENVVTMIEESTALALV
                     GTEAGVRTSFQFDPKAEWVFADRVQVQQVLVNMMRNAVEAMAGCRRRELEVRTALLDD
                     QSVEIAIVDSGPGLAKSVADHLFEPFVSTKPNGMGLGLSICCS"
BASE COUNT          462 a          662 c          697 g          401 t
ORIGIN      
        1 agggtgagcc gccggtgcgc gagcgtgatc agttcgtcgc gccggaagcc ttggaggacg
       61 cggttcgtgt gcaccgaggt cagaccgagc atgtcggcca gatccgtctg cgtaaagggc
      121 agccggatcg cgtgatcctc cgccatgccg atcgcccgct gccgccaaac gagctcgcac
      181 aaaatatagg cgacgcgccc gcgcgccgag cgccgtccga gcgcgacgat gcgctcccgc
      241 agcatggcct cctcctgcat cgcgctccac cacagagcgg cgccgatccg tggatggttg
      301 gcgatgatgt cgatcaccac ctctcggccg attgcggcga tccgcgtcgg gccgatggcg
      361 gcgacggagt ggtccatgac cttcagcagg aacccgtgca ggtcgaagaa gtcgccgggt
      421 atcaggatcg tcaggatctg ccgccttccg tcgggcatca ggcggaaacg gcacgccatt
      481 ccccgcgtga gaacaaacgc cgagcgcggc gtttcaccct cgaccacgat gttggcgcca
      541 gcgcggaagc gttcttccgg caaacacagc gtctcgagca gggcggcatc ctggtccgat
      601 agcggcgcga attgcgagag cttggctacg agcggattcc tggctcgtcc attgtcagcg
      661 gccattccac gcctcgatca gacagtatgt cgctatcgcc ttcgccccaa gaaggccgct
      721 ctgtgcccat gtttggacgg gcaaccagcc gcgcctgccg ttcacccctt gccggtcacg
      781 caacccgcgg ctgcaccagg ggtgtttagc agagatcccc aggttatatc attaaactgg
      841 attagtatcg gaggcgggcg gtcgccccca gttcgagccc tgagtgttcg tggttgccag
      901 atacgcagag gctcggcttt gcagacactt ataaaataat tcttgatcca gggcaataac
      961 tcggcccggt cactgaatga attagttaac aaaaatatgc atccgctcgg atctagtgtc
     1021 aacggctggt gttgtctcgc tgatcccgag cgcgttaggg gtgcataaga gcggcacgga
     1081 ggttgaatga tgaattcttc gtctctcgat tattggcaat cggccgccgc tgtcgtcgaa
     1141 tcctcgaatg acgctatcat atgcacagac ctgagcggga cgattacaag ctggaataag
     1201 gccgccaaaa cgatcttcgg gtatcggcca gaggaggtca tcgggcagcc tgttttcctg
     1261 ctggccgcgc cgggccgcga agacgagatg cctgttatcc tcgatcagat aatacggggc
     1321 gaaaagatac agcattacga aacggtacgg tgccacaagg agggccgcca agtcgccatt
     1381 tcgctatccg tctccccgat ccgcgaggag agcggccgga tcgtcggcgc ctcgaagatc
     1441 gccagggaca tcaccgagcg cacggaggcc gaagccgaat tgcggcgcct gaacaagacg
     1501 ctggaacagc gggtggccga acggaccgcc gagcttgaag aggtaaaccg gcaattgcaa
     1561 gccaagattg ccgaaaaaga gcgtacggat cggcggttga gggaactgca atcggaagtt
     1621 ttccacgccg cgcgcctcag cgctgtcggt caaatggctg ccaccttggc gcatgagctg
     1681 aaccaggtca tgaccgccgc caccaatttc gtcaacgcgt ctcgtcgcct gctctcgagt
     1741 ggagaccgcc acaagatcga aacggtcccg aaggtcatgg acgaggcggc agcggaaata
     1801 ctgcgcgccg gacgcattat cggacgcctg cgagccttcg tcagcagggg agagacggaa
     1861 cggcgcgtcg agaacgtcgt cacgatgatc gaagaatcga ccgccttggc cttggtcggg
     1921 actgaagcgg gtgttcggac gagcttccaa ttcgatccga aagccgagtg ggtcttcgcc
     1981 gacagggtcc aggtccagca ggtgctcgtc aatatgatgc gcaacgcggt cgaggcgatg
     2041 gccggatgca gacgccgcga actagaggtg aggaccgcat tgctcgacga ccaaagcgtc
     2101 gagatcgcga ttgtcgacag cgggccgggt cttgccaaat cggtagccga tcacctcttc
     2161 gagccgttcg tctcgacaaa accgaatggc atgggtctcg gcctgtcgat ttgctgctcg
     2221 at
//
LOCUS       DATAQL010000760         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2215558, whole genome shotgun sequence.
ACCESSION   DATAQL010000760 DATAQL010000000
VERSION     DATAQL010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215558"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VHS97_13240"
     CDS             <1..787
                     /locus_tag="VHS97_13240"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3449217.1"
                     /translation="SESNFAVDPANRLIWRHSPRRLSAEEIRDATLAAASKLDLGRPD
                     GSPAKDLKVTEMANNGPLARRIGEQASKSALRSVYLPLLRGVTQTSLEVFDFAEQGMV
                     TGSRDTTTVATQALYLLNDPFVRRQSLALAERLLETTEADSSNRIDRAYRLTIGRPAS
                     AAEIERARTYLANYVSSARELVASIPPVHEQPPSTVVAAADADASAKAAAKRPAPPVN
                     PDEVEQVEVPVKEEVIAAANPETAAWASFCQALLGSAEFRYIK"
     gene            849..2255
                     /locus_tag="VHS97_13245"
     CDS             849..2255
                     /locus_tag="VHS97_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3449218.1"
                     /translation="MPFTQSKTTGIGTEISRRQVLRTAGTGFSYLALAGMLGQNVAKA
                     RDGTASHALEPRPLAPKPPHYPVKAKRIIFLFMNGAISQMDTWDYKPQLQKDDGKVGP
                     GGGVLTGSKFRFSQHGETGTWVSELYPRTAQHVDKLCFIRGLHTDTPAHPEAVIQLHT
                     GAALASLTRPSLGAWLLYGLGTENQDLPGYVTINPPPNFGGAVNYGSAFLPAHFQGTR
                     INDVGYVPNLKAQTALALQRKQIDLVQAMNRDLRGTPGAPDQLDGVIQSYELAFKMQG
                     KVPELLDISSEPQSVLDMYGVKPGPAGSFARQCLMARRLSEAGVRFVEVAQPGWDHHT
                     NLHQGLIRNSAYTDQPTAALLADLDQRGLLDETLVLFGSEFGRLPTAQGPDGRDHNIT
                     GYPMWLAGAGVKKGFSYGASDEYGINAVEGRMHSTDLHATLLALMGLDHERLTYRYAG
                     RDFRLTDVAGSVVTEIMA"
     gene            2564..3004
                     /locus_tag="VHS97_13250"
     CDS             2564..3004
                     /locus_tag="VHS97_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEX3449219.1"
                     /translation="MASMTWIRDSDNEMTHSDAIEVKRVKGKGRGVFALRSIRDGELI
                     ERVPVVVLKTNDVKDSEGWTGLAAYCFHWTKGTLALALGYGSLYNHSYRPNARYDDLE
                     GQIKEFIAIRNIEPGEEITVNYNGEPEDDSPVGFKVVMREQPVT"
BASE COUNT          612 a          904 c          954 g          581 t
ORIGIN      
        1 gtcggagtcc aatttcgctg tcgaccccgc gaacaggctg atctggcggc atagcccgcg
       61 gcggctcagc gcggaggaga ttcgcgatgc cacgctcgca gcggccagca agctcgacct
      121 gggccggccc gacggttcgc ccgccaaaga tctgaaggtc acggagatgg caaacaacgg
      181 tcccctggcg cggcgaatcg gcgaacaagc aagtaagagc gcgctccgta gcgtttatct
      241 gccgctcttg cggggtgtaa cgcagacgtc gctcgaggtc ttcgatttcg ccgagcaagg
      301 catggtgacc ggcagccgcg atacgaccac ggtcgctacc caggcccttt atctgctgaa
      361 cgatcccttt gtacggcgtc aatctctggc cctggccgaa cgactgctcg agacaaccga
      421 ggcggacagt tccaaccgca tcgaccgggc ctaccggctg acaatcggca ggccggcgtc
      481 cgcggccgag atcgagcgag cgcgcaccta cctcgcgaac tacgtgagct ccgcgcgtga
      541 gctggtcgcc tctatccccc cggtccacga gcagccgcca agcacagtcg tggcggcggc
      601 tgacgccgat gccagcgcca aggctgccgc caagcggccc gcgccgcccg tgaatccgga
      661 cgaggtcgag caggtggaag taccggtcaa ggaagaagtc atcgcggcgg ccaatccaga
      721 gaccgctgcc tgggccagct tttgccaggc gctcctgggg agtgcggaat ttcgttatat
      781 caagtgagac gaattacccg atgtcttcat aaaatcatct gatagtcagt gagttgagag
      841 ggtgacgcat gccgttcacg caatccaaga cgacggggat agggactgag atttcccgcc
      901 ggcaggttct gaggaccgcc ggcacgggtt tcagttacct ggccttggcc ggaatgctcg
      961 ggcagaacgt ggctaaggca cgtgacggaa cagccagcca cgcgctcgag ccccgccccc
     1021 tcgcgcccaa gccgccgcac taccccgtga aggccaagcg gatcattttt ctgttcatga
     1081 acggtgccat ttctcagatg gatacctggg attacaagcc gcaacttcag aaggatgacg
     1141 gcaaagtggg gcccggcggc ggggtcttga ccgggtcgaa attccgattc agccagcatg
     1201 gcgaaaccgg cacgtgggtc tccgagctgt accctcgcac cgcccagcat gtcgacaagc
     1261 tgtgctttat ccgcggccta cacaccgata cgcctgccca tcccgaagcc gtgatccagc
     1321 tgcacacggg agcggccttg gcgtcgctga cccggcctag tttgggcgcg tggttactct
     1381 acggtctcgg aacggaaaac caggacctac ccggttacgt cacgatcaac ccgcccccca
     1441 acttcggcgg agcggtgaat tacggcagtg catttctgcc ggcccatttt caggggaccc
     1501 gaatcaacga tgtcggctac gtgcccaatc tgaaagcaca aacggctcta gcgcttcagc
     1561 gcaagcagat cgatctggtc caggcgatga atcgcgacct gcgggggacg ccgggtgcgc
     1621 ccgatcagct cgacggtgtg atccagtcgt acgaactggc gttcaagatg cagggaaaag
     1681 tcccggagtt gctggacatt tccagcgaac cgcaatcggt gctcgacatg tacggtgtca
     1741 aacctggccc ggcggggagc ttcgcgcgtc aatgcctgat ggcccggcgg ctgagcgagg
     1801 cgggggtccg gtttgtcgag gtcgcgcagc cggggtggga tcatcacacg aacctccacc
     1861 agggcttgat acgcaatagc gcctacaccg accagcccac agcggcgctg ttggccgatc
     1921 tcgaccagcg ggggcttctc gacgagactt tggtattgtt tggcagcgag ttcggccggt
     1981 tgccgactgc tcagggcccg gacggccgcg atcacaacat taccggctac ccgatgtggc
     2041 tggctggagc cggcgtcaag aagggctttt cgtatggcgc cagcgacgaa tacggtatta
     2101 acgcggtcga aggccggatg cattcgacag acctgcatgc cacgctctta gcgctcatgg
     2161 ggctggacca cgagaggcta acctaccggt atgccggtcg ggacttccga ctgaccgatg
     2221 tggccgggag cgtcgttacg gaaatcatgg cttagtcgct tcgcgattgg ttgtttttta
     2281 gtagtcctca atgtatcgag gctttggcga gcgtgaccca tgcgctggtc gcgccggtga
     2341 aatcgccgcc atcgacgttg atggtaagct tacctgccgg gaggcaaagg ccttgtcctc
     2401 ctggcattgg ctttcatgca agaatccccg tcgtcgcccc acctgccgtg agtttgtcgt
     2461 gccgcaattg ccactttctc tgtccaccaa gtcaaaggag cgatatcctt tggcggatga
     2521 ggtacatttt tttcacggca acaaatggcc aggagggtct ggcatggctt ccatgacttg
     2581 gattcgggac agtgataatg agatgacgca ttcggatgcg atcgaggtca agcgggtcaa
     2641 ggggaaaggg cgcggcgttt ttgccctccg ttcgatccgc gatggggaat tgatcgaacg
     2701 ggtcccggtc gtggtgttga aaaccaacga cgtgaaggac agtgagggct ggaccggcct
     2761 ggcggcctat tgcttccact ggaccaaagg gacgctggca ctggcacttg gttacggctc
     2821 actttacaac cattcgtacc ggcccaatgc ccggtatgac gacttggagg gacaaatcaa
     2881 ggagttcatc gccatccgaa atatcgaacc cggcgaggag atcacggtca attacaacgg
     2941 tgagccggag gacgattctc cggtcggttt caaggttgtt atgcgcgagc aacccgtcac
     3001 gtaggcagaa tgacatttcg atttcgtcaa ttgtctgaag cgggtggctg g
//
LOCUS       DATAQL010000761         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2369570, whole genome shotgun sequence.
ACCESSION   DATAQL010000761 DATAQL010000000
VERSION     DATAQL010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369570"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VHS97_13255"
     CDS             <1..314
                     /locus_tag="VHS97_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449220.1"
                     /translation="LAMENPFAAEPSRPDVVRFVSILSKTSGFQASIPVTLPPSGEWL
                     VRLLATKGRFVFGVYRRHMKTISYLGQVDKLFGVPATTRNWNTIVAILRILKGQPKKD
                     R"
     gene            complement(453..1331)
                     /gene="lpxI"
                     /locus_tag="VHS97_13260"
     CDS             complement(453..1331)
                     /gene="lpxI"
                     /locus_tag="VHS97_13260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248553.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX3449221.1"
                     /translation="MSAIAHSKRVSRLAGGGHAEPIGLLAGSGRFPILFAKAARRQGL
                     PVACMGIKYEASEELRGLCDSFQWVGVAKLGGMIRGFRRQGARRIVMAGKVTKNVIYT
                     PWRVLQLWPDARTVQWWYRRNRVDNRDDSLLLSLIAEFERDGMTFESALDFCPELLVK
                     NGILTRRAPTSAEQNDIQFGWDLAKEMGRLDVGQSVAIKEKAALAVEAIEGTDRCIER
                     AGQLCKAGGWTLVKVAKPQQDMRFDVPTIGVTTIENLHKARARVLAIEAGQTILLDQP
                     EVVALADRYGLCIVAI"
     gene            complement(1377..>1750)
                     /locus_tag="VHS97_13265"
     CDS             complement(1377..>1750)
                     /locus_tag="VHS97_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449222.1"
                     /translation="QAGIKDKTQIGDRVKVGAQAGVHRNIPDGQTVLGSPAVPVREQR
                     RIFQMIARLPEMHRQLRELTAQIEALTALAAQGSGALNGLANDHAAISAGGTDLLPMN
                     SIGHGLEACATRAEDSDNESE"
BASE COUNT          331 a          547 c          519 g          353 t
ORIGIN      
        1 gcctggcgat ggagaatccg tttgcagcgg agccctcacg ccctgacgtg gttcgattcg
       61 tcagcatcct gtcgaaaacc agcggctttc aggcctccat tccagtcaca ttaccgccaa
      121 gcggggagtg gctcgtgcga cttctcgcga cgaaaggccg attcgttttc ggagtgtacc
      181 gtcgtcacat gaaaacaatt agctatctcg gccaggtcga caaattgttc ggagtgccgg
      241 ccacgacacg aaattggaac acaatagttg cgatcctgcg gattttgaaa ggccagccga
      301 agaaagaccg ctgatccgcg tatagcgaca gcccgcacaa gtcttctacg ttctcgccga
      361 ggggctcgtt tctgaaaaaa gtcggggcgg caggattggg acctgcgacg gcgcgatttc
      421 aacgcgcgtg aggttgtaat aaaacggcaa cgtcaaatcg ccacaatgca aaggccatag
      481 cggtcggcaa gcgcgacgac ttcgggctga tcgagcagga tcgtttgtcc ggcttcgatt
      541 gcgagcacgc gggcgcgggc tttgtgtagg ttttcgatgg tggtgacccc gatcgtcggc
      601 acgtcgaacc gcatgtcttg ttgcggcttg gccactttga cgagcgtcca gccgccggcc
      661 ttacaaagtt ggccggcgcg ctcgatgcag cggtcggtcc cctcgatcgc ctcgacggcc
      721 agcgcggcct tttccttgat cgccaccgac tggccgacgt cgagccggcc catttccttg
      781 gcgagatccc agccgaactg gatgtcattc tgctcggccg acgtgggcgc gcggcgagtg
      841 agtatgccgt ttttcactag cagctccggg caaaaatcca gcgcagactc aaacgtcatt
      901 ccgtcacgtt caaactcggc aatcaagcta agcaagagac tatcgtcgcg gttgtcgacg
      961 cgattacggc ggtaccacca ttgaactgtg cgagcgtcgg gccagagctg aagcactcgc
     1021 cacggggtgt agatcacgtt tttcgtgacc tttcccgcca tcacgatccg tcgggccccc
     1081 tggcggcgaa agccccggat cattccgccg agcttggcga ctccgaccca ctgaaacgag
     1141 tcgcagagac cgcgcagctc ttcggaagct tcgtacttga tccccatgca tgccaccggc
     1201 aggccttgcc gcctagctgc cttggcaaac aatatcggga atcggccgct gccggccaag
     1261 aggccgatcg gctcggcgtg cccgccgcca gccagcctcg agacacgctt tgaatgggct
     1321 atcgcgctca cgcgaaatcg ctcccggaaa tgtcggtttg ggacacgaaa agccagttac
     1381 tctgactcgt tgtcagaatc ttcggccctt gtggcacagg cttccagccc gtgtccgatc
     1441 gaattcatgg gcaagaggtc cgtgccgccc gcagaaatcg cggcgtggtc gttcgccaga
     1501 ccatttagtg cgccggaccc ttgcgcagcc agcgcagtca gtgcctcgat ctgggccgtg
     1561 agctctctga gctgccggtg catctcgggc agccgggcga tcatctgaaa aatccgccgc
     1621 tgctcccgca ctggaaccgc cggcgagccc agcaccgttt gcccatcggg gatgttgcgg
     1681 tgtacgcccg cctgggctcc gaccttcacg cgatcgccga tctgcgtctt gtctttgatg
     1741 ccggcttggc
//
LOCUS       DATAQL010000762         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4657530, whole genome shotgun sequence.
ACCESSION   DATAQL010000762 DATAQL010000000
VERSION     DATAQL010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4657530"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VHS97_13270"
     CDS             complement(<1..1017)
                     /locus_tag="VHS97_13270"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3449223.1"
                     /translation="MKIPMPMGSPLWIAAGWTMFHFYWVGAAIGLSGLLGRRALRGAR
                     PEARYALALTILAAMTVAPAAIFAWVYERSPGSSPIRTTSASKALLPERPMLEHHSIP
                     LLEAQSPSPAAPRRQWQSPVNALVGFLPWLWLAGSPVTFVLLACALVGAERFKRQSRM
                     IESGEIPRLCQRLARALSISRRVAVGVCDQLAAPVLIGILRPVILLPPAALNGWTIEQ
                     LEMVLWHELAHLRRWDNLINLVQRIVESLLFFHPVVWWISAWARLEREYCCDLIVVQR
                     TGRPRSYAETLAALAGAPAPRSQPFAMALAESQVVDRIRRILGIHEHYMTMKLSRVSV
                     TVTTA"
     gene            complement(1014..1427)
                     /locus_tag="VHS97_13275"
     CDS             complement(1014..1427)
                     /locus_tag="VHS97_13275"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3449224.1"
                     /translation="MARPRSSQPTDGEMEILKVLWAVGPAELGQIRAQLQKQRPVATT
                     TIATMLKVMLGKKLVKRSDGKRGYVWSARASRTRTASELVRKVLDHVFDGSAGRLVAH
                     LLEETELDRRDRDEIRKLLEAFDAREVGSPGETPP"
     gene            1742..>2554
                     /locus_tag="VHS97_13280"
     CDS             1742..>2554
                     /locus_tag="VHS97_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449225.1"
                     /translation="MGEQRPGKWVVPNPQIPRTFGIMNLVFGILMFLVGAGYIAITIV
                     APSFQKQMVVTFEQQQAKSKAEREAKVAELKAKEEAAKTKEEKDTLKDEREAAESYVQ
                     PDLSAMNEMMGFNIFSDIRIAIYTFSELISGMILNVLMVISGVGLLGLAEWGRRLAIT
                     VAWLKIVRWVAMIVVTLVLIVPITAAKTQKMFDSIQQAQARAQANAGGRGVATMPMLN
                     IAAFTSIAGAVTMIFTTLVASIYPALSIWFLTRPPVRAACLQRSKPPSAPPGM"
BASE COUNT          525 a          750 c          773 g          506 t
ORIGIN      
        1 cgctgtcgtg acagtcaccg acacacgcga gagcttcatc gtcatgtaat gctcgtgaat
       61 tccgaggatg cggcgaatgc ggtcgacgac ttggctttcc gccagtgcca ttgcgaacgg
      121 ttggctgcgc ggtgccggcg ctcccgcaag agcagctagc gtctcggcgt aagaccgagg
      181 acggcctgtt ctctgtacaa caatcaggtc gcagcaatac tcgcgctcca gccgcgccca
      241 cgccgaaatc caccagacaa ctggatgaaa gaacaagagc gattccacga tccgttgaac
      301 gagattgatc aggttatccc accggcgtag gtgggccaac tcgtgccaga gaaccatttc
      361 gagctgctcg atcgtccagc cattcaacgc ggcgggcggc agcaggatca ccggccgcag
      421 gataccgatc aatacgggcg cagccagttg gtcgcagacg ccgacggcga cccgccggct
      481 aatggaaagc gcccgcgcca gacgttggca gagccggggg atctcgcccg attcaatcat
      541 gcggctttgt cgcttgaatc gttcggctcc gacaagcgcg caggccaaga gaacgaacgt
      601 caccggcgag ccggcgagcc agagccaagg gagaaacccc accagtgcat tgacgggcga
      661 ttgccactgc cttcgcggcg cggctggact cggcgattgg gcttcaagca gcggtattga
      721 gtgatgctcg agcatgggtc gttcgggcag taatgctttc gacgcggacg tcgtccgaat
      781 cggcgaagag cccggcgacc gctcataaac ccacgcaaat atcgcggcgg gagccacagt
      841 cattgcggcc aggatggtga gcgccaaggc atagcgtgcc tctggcctgg cgccgcgaag
      901 cgcgcgcctc cccaagaggc cggaaagacc gatcgcggcg ccgacccagt agaaatgaaa
      961 catcgtccag cccgcagcga tccagagcgg tgaacccatc ggcatcggga ttttcatggc
     1021 ggtgtctccc ctggggaacc gacctcacgg gcgtcaaaag cttcgagcaa tttccgaatt
     1081 tcgtcacgat cgcggcggtc gagttctgtt tcctcgagca ggtgggcgac cagacgcccc
     1141 gccgacccat caaagacatg gtccagcacc ttacgaacca gttcgctcgc agtccgggtg
     1201 cgactggcac gggccgacca gacgtagccg cgcttgccgt cggaccgctt aaccagcttt
     1261 ttgcctagca tgaccttgag catcgtcgcg attgttgtgg tcgccaccgg ccgctgtttt
     1321 tgcaactgcg cgcgaatctg ccccagctcg gcgggcccga cggcccaaag cactttcaag
     1381 atctccattt caccatcggt cggctgtgag cttcttggtc gtgccatttt cgagcatggt
     1441 ccttgggtgt ccttgatatc tcggttgaca ttctaacgaa ttagaatcgc ctgtcaatcg
     1501 agaacggagc tttccaggcg cattattatc tgtgacaact gtcagataat ttgtcgtcaa
     1561 ccgctggcgt tggaagggga tttctcaata tcatggaacc tcaggcgaag acccacttgg
     1621 atttgagaat actggcgttt ctccggtacg ccgcccgcaa gccacggtgg cttttgcgct
     1681 tccagaacga tcgttcggtt cccggagtgt tgtttgtcgc agcgatcagg agccgcaggc
     1741 aatgggagag cagcgaccgg ggaaatgggt ggtacccaat cctcagattc cgcgcacgtt
     1801 cggcatcatg aatctcgttt tcggcattct gatgtttttg gtcggagccg gatatatagc
     1861 gataacgatt gtggcaccgt cctttcaaaa gcagatggtg gttacgttcg agcaacagca
     1921 ggccaagagc aaggctgagc gtgaggcgaa ggtcgccgag ctcaaggcca aggaggaagc
     1981 cgccaagacc aaggaggaga aagatacgct caaggacgaa cgggaggctg ccgagtcgta
     2041 cgttcagccc gacctctccg cgatgaacga gatgatgggt tttaatatct tcagcgacat
     2101 ccggatcgcg atctatacat tctccgaact gatctcgggg atgattttaa acgtgctgat
     2161 ggtgatttcg ggtgtgggtc tcttgggatt ggccgagtgg gggcgccggc ttgccatcac
     2221 tgttgcctgg ctcaagatcg ttcgctgggt agcgatgatt gtggtgactc tcgtcctgat
     2281 cgtgccgatc acggccgcga agacccagaa gatgttcgac tcaatccagc aggcccaggc
     2341 tcgagcccag gccaacgcgg gcggtcgagg ggtggcaaca atgccgatgc tgaacatcgc
     2401 tgccttcacg tcgattgcag gcgcagtcac gatgatcttc accaccctgg tcgcgtcgat
     2461 ctacccggct ctctcgatct ggtttctgac gcggccaccg gtccgcgcgg cctgcttgca
     2521 acgatcgaag ccgccgtcgg ccccgcccgg gatg
//
LOCUS       DATAQL010000763         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4047722, whole genome shotgun sequence.
ACCESSION   DATAQL010000763 DATAQL010000000
VERSION     DATAQL010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4047722"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1971
                     /locus_tag="VHS97_13285"
     CDS             <1..1971
                     /locus_tag="VHS97_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX3449226.1"
                     /translation="QAAADQVPPLLINDPAPIKELSARLDDLTTAVAKSTRFEDLNEN
                     VRAVWKLNPESYLDLKAATDTPERRDNLHVQIAAAFAALLRDGVLGPGTLPPNEESSR
                     LLSVRTADQPRSLARVVPRDRVVPERIVKPDGPVYKEFCSVFTSVRVGQILFGLIANQ
                     LDNSPTLSFEAEATAQLREEARNSVPDHYDPYTRGEVLVEEGQLIGEEQLILLRLEHD
                     AAVAELGFGDRARRAVGIFALVTALFFLAGSYVHKHERQFVREPARVAMICGLVILAL
                     AVVRILAAQTWHAEVVPVAIAAMIVAIAYNQSFAILVTFGLSVLTSIALGTEIPHFLV
                     LMGGTVAGVLTLNDVRTRTKLIKVGATAAFTYFLMTWATGLWQDEPVELVRNDSFWRA
                     GWGLMAGFFLGGSLPFIENALGIVTGISLLELGDNTHPLLQELVRRAPGTHNHSITVG
                     AIAESAAERIGANALLVRIGAYFHDIGKMLKPHYFVENQVGPISRHAKLAPAMSTLII
                     IGHVKDGVDLGRQHHLPEPIIDLIEQHHGTTLVEYFFHEANRRNGSNPDASPVPESAY
                     RYPGPKPQTKEAAILMVADAVESASRALSDPTPARLEGLASDLIDKRLRDGQFDECGL
                     TLREIAEIRESLIKSLIGIYHGRVKYPEHRTA"
     gene            2074..>2404
                     /gene="ybeY"
                     /locus_tag="VHS97_13290"
     CDS             2074..>2404
                     /gene="ybeY"
                     /locus_tag="VHS97_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX3449227.1"
                     /translation="MGALIEVEISDTQGQLRVDRAALEQLVRVVLAAEDRARASISIA
                     LVDNASIHAVNQTYLGHDWPTDVISFPLSAPDDPVLTGELVISAEMACTSAQEIGVDS
                     QHELALYV"
BASE COUNT          450 a          791 c          706 g          457 t
ORIGIN      
        1 caggcggccg ccgatcaggt cccgccgcta ttgatcaacg atccggctcc gatcaaggaa
       61 ctctccgcgc ggctcgacga cctgaccacc gccgtggcga agtcgacacg gttcgaagat
      121 ttgaacgaaa acgtgcgagc cgtctggaaa ctcaaccccg aaagttatct cgacctcaag
      181 gcagccaccg acacccccga gcgccgcgac aacttgcacg ttcaaattgc cgccgctttt
      241 gcagctttgc ttcgcgacgg tgtgctcggg ccgggaacct tgcctcctaa cgaagaatcg
      301 agccggctcc tgtcggtgcg cacggcggac cagccacggt cccttgctcg ggtggtacca
      361 cgcgaccgcg tggttcccga acggatcgtc aagcccgacg gcccggtcta caaagagttc
      421 tgctcggtgt tcacttcggt gcgggttggg cagatcctct ttggtctgat cgccaatcag
      481 cttgataatt cacccactct gtcgttcgag gcggaggcga ctgcccagct tcgagaagag
      541 gcgcgcaaca gcgtccccga ccattacgac ccgtatacgc gcggcgaggt gttggtcgag
      601 gaagggcaat taattggcga agagcagctc atcctgctgc gcctcgagca cgacgccgcg
      661 gtcgccgagc tcggttttgg cgaccgggct cgccgcgccg tcggaatctt cgcgctcgtg
      721 actgcgctct ttttcctggc cggatcgtac gtccataagc acgaacgcca gtttgtccgc
      781 gagcctgcgc gcgtcgccat gatctgcggt ctggtcatcc tggccttggc cgtcgtccgc
      841 atcctggccg cgcagacctg gcatgccgaa gtggtcccgg tcgctatcgc ggccatgatc
      901 gtggcaatcg cctataatca gagcttcgcg atcctggtaa ccttcggtct gtcggtcttg
      961 acgtcgatcg cgctcggaac cgagattccc cattttctgg tgctgatggg gggaaccgtg
     1021 gcgggcgtgc tgaccttgaa cgacgtacgc acccgcacca aactgatcaa ggttggcgcc
     1081 acggcggcct tcacctactt tctcatgact tgggccactg gcctgtggca ggacgagccc
     1141 gtcgaattgg tacgcaacga cagtttctgg cgcgccggct gggggctcat ggctggcttc
     1201 tttctgggcg gaagtcttcc gttcatcgaa aacgccctgg gaattgtcac gggcatcagc
     1261 ctcttggagc tcggcgacaa tactcacccc cttctccagg agttggttcg ccgcgccccg
     1321 ggcacgcaca accactcgat cacggtcggc gccatcgccg agtcggccgc cgaacgcatc
     1381 ggggccaatg ccctattggt ccgcattggg gcttattttc acgacatcgg caagatgctc
     1441 aagccccact actttgtcga gaaccaggtc ggtcccatca gccggcacgc caagctggcc
     1501 cccgcgatga gcacactcat catcatcggc cacgtgaagg acggcgtcga cttgggccgg
     1561 cagcaccacc tacccgagcc catcatcgac ttgatcgagc agcaccacgg gacgactctc
     1621 gtcgaatact tctttcacga ggccaatcgg cggaacggca gcaatcctga tgcttcgccg
     1681 gtacccgaaa gcgcttatcg ctatcccgga ccgaagccac agaccaaaga ggctgccatc
     1741 ctgatggtcg ccgatgccgt cgagagtgcc agccgcgcgc tttccgatcc gacgcctgcc
     1801 cggctggagg ggctggccag cgacttgatc gacaagcgcc tccgcgacgg ccagttcgac
     1861 gagtgcgggc tcaccctccg cgaaatcgcc gaaatccgcg agagcctgat caagtcgctt
     1921 atcgggatct accacggacg cgtcaaatac cccgagcaca ggacggcatg agacgtgcta
     1981 ctgacctcaa tgggccggtt gcacgagcct cgctcgctcg atcgagaaac agcggaaact
     2041 ttcaagcggg cgctccgatt cggaatatca cacatgggcg cccttatcga agtcgaaatc
     2101 agtgatacac aaggccagct gcgcgtcgac cgcgccgctc tcgaacagct cgtacgcgtc
     2161 gtgcttgccg cggaggatcg cgcgcgcgct tcgatatcga tagcgctcgt cgataacgcg
     2221 tcgatccacg ccgtgaatca gacatatctg ggccacgact ggccgacgga cgtcataagt
     2281 ttccccttga gtgcacccga tgacccggtt ctgacgggcg agctggtgat ctccgccgag
     2341 atggcgtgca cgagcgcgca ggagatcggc gtcgattccc agcatgagct cgccctttac
     2401 gtgg
//
LOCUS       DATAQL010000764         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2674652, whole genome shotgun sequence.
ACCESSION   DATAQL010000764 DATAQL010000000
VERSION     DATAQL010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674652"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VHS97_13295"
     CDS             complement(<1..469)
                     /locus_tag="VHS97_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449228.1"
                     /translation="MLLAPQSNPMIDAIITFSIRNRALVIGASLVLAALGAWAAWDTP
                     VDAIPDLSENQVIVFTEWKGHGPREIEDQVTFPLTLGLRGLRGVRVVRSSSDVGFSMI
                     SVIFEDGVPIAEARRRVAERMARLGGQLPQGAAPELAPDAPATGQIFWYTVEGG"
     gene            complement(486..1691)
                     /locus_tag="VHS97_13300"
     CDS             complement(486..1691)
                     /locus_tag="VHS97_13300"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX3449229.1"
                     /translation="MRIPIVLVIAALLVGRWDVIRNHWDRLTRGALSESISSHAVSAD
                     TEYFCPMDPGVVSDWPGKCGICNMALVRRKRGEAQALPNGVIARMQLSPYRIQLAGIQ
                     TAPAKFRPLVRTFESSGVVAREADVATVRLEIPGRQAPWIAQGQTADVTCHELAGRDP
                     LSGLVQAVGNHSAAGWDFLSATITITDPPRDLVAGMIAVVRIKVPMAGIEPFRSLPAD
                     PPRLVASESRQAYVCPDHPANLTAEPGRCPIDHKEREPRPLSDHQRLRWWCPMHPSVT
                     ADQPGASCKACGGMILVPRVISYSPAGQVLSVPQSAVVDAGACKVVFVESMPGMFDGV
                     EVVLGPRCGDTYPVVQGLEPGQRVAIAGAFLLDAETRLNPSLAAGYFGAGRGERAVVP
                     GPIAGANKR"
     gene            complement(1796..2266)
                     /locus_tag="VHS97_13305"
     CDS             complement(1796..2266)
                     /locus_tag="VHS97_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449230.1"
                     /translation="MTRALRPGFRAFITVLPFVAMFIGCDTSQARYKPTSSEARSSLE
                     AALTAWRDGKPYGPIASTPPIQVGDTAWQAGQQVESFEIGDEEDNGDGTKQFTVKLKM
                     KKPAGEQSVRYVIHGRDPVWVYREEDYKRMANMENNPVPASGSKSASRRSGRSR"
     gene            complement(2288..>2431)
                     /locus_tag="VHS97_13310"
     CDS             complement(2288..>2431)
                     /locus_tag="VHS97_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEX3449231.1"
                     /translation="PSSNHPGGVNVLMGDASGRFIKSTVNVQTWRNVGTRNGGEVISA
                     DGY"
BASE COUNT          449 a          776 c          769 g          437 t
ORIGIN      
        1 ccccgccttc caccgtgtac cagaagatct ggccggtcgc cggcgcatcg ggcgccagct
       61 cgggcgcggc gccttggggc aactgacctc cgagtcgggc catgcgctcg gccacacgcc
      121 tgcgcgcctc ggcgatggga acgccgtcct cgaagatcac gctgatcatc gagaaaccga
      181 cgtcgctgga tgaccgcaca acccgcacgc cgcgcagccc gcggaggccg agagtgagcg
      241 gaaaggtcac ctgatcctcg atctcgcgcg ggccatgtcc cttccactcg gtgaagacga
      301 tgacctggtt ttccgagagg tcgggtatcg cgtcgaccgg cgtatcccag gccgcccatg
      361 cgcccagggc ggccagcacc aagctggcgc caatcacgag ggcacgattt cgaatcgaga
      421 aggtgatgat cgcatcgatc atgggatttg attgcggcgc gagcaacatg cccgtgcctc
      481 aagtctcaac gtttgttggc tccggcgatc ggccccggaa ctacggcgcg ctcgcctcgg
      541 cccgctccga agtagccggc cgcgagactg gggttcaagc gtgtctcggc atcgagcaga
      601 aacgcaccgg cgatcgcgac gcgctggcca ggttcaaggc cctgaacgac tggataagtg
      661 tcgccgcaac gcggacccag gacgacctcg acgccgtcga acatgccagg catgctctcg
      721 acgaagacaa ccttgcacgc accggcgtcg acgaccgccg attgcggcac gctcagcact
      781 tggcccgctg gactgtacga aatcaccctg ggcacgagaa tcatgccgcc gcaagctttg
      841 cagcttgccc caggctggtc ggccgttaca ctcggatgca tcgggcacca ccaacgtaga
      901 cgctggtggt cggagagcgg acggggctcg cgctctttgt ggtcgatcgg acagcggccc
      961 ggttcggcgg tcaggttggc gggatggtca gggcagacgt aggcttgtct ggactcactg
     1021 gcgaccaacc ggggcgggtc ggctggcaat gagcggaagg gctcaatccc ggccatcgga
     1081 accttgatgc gaacgaccgc gatcatcccg gcgacaaggt cgcgcggggg atcggtgatt
     1141 gtgattgtgg cgctcaggaa gtcccagccc gcggcggaat gatttcccac ggcttgcaca
     1201 agacccgaaa gtggatcacg gcccgccagt tcgtggcagg taacgtccgc cgtctggccc
     1261 tgggcgatcc agggtgcctg ccgcccgggt atctcgagcc gcacagtggc gacgtccgct
     1321 tcacgcgcca cgacaccgga cgattcaaac gttcgcacga gcggccgaaa cttggcaggc
     1381 gcggtctgaa ttcccgcgag ttgaatccgg tacggtgaaa gctgcatccg cgcgatgact
     1441 ccattgggaa gcgcctgagc ttcgcctcgc ttgcggcgca ccaaggccat gttgcagatc
     1501 ccacacttgc ccggccaatc agagaccact ccgggatcca tggggcaaaa atactcggta
     1561 tcggccgaca cggcgtgaga tgagatgctt tccgaaagcg cgccccgggt caatcgatcc
     1621 cagtggttgc ggattacgtc ccagcgcccg accaagagcg ccgcgatcac gagcacaatc
     1681 gggatacgca tccgcacctg ggccagcttg agtgcagacc aggtccggcg ccaaatcgga
     1741 ctcgccgcgc tggaattggt gtggctgtcc gttatggaac catgtgcaag tctagtcatc
     1801 gcgacctgcc ggatcgccta gaagcggact tcgagccgga agccggcacg gggttgtttt
     1861 ccatgttggc catccgtttg taatcttcct cgcggtagac ccaaaccggg tcccggccgt
     1921 ggataacata gcggacagac tgctcgcccg cgggcttctt catcttgagc ttgacggtga
     1981 actgcttggt tccatcgccg ttgtcctcct cgtcgccgat ctcgaacgac tcgacctgtt
     2041 ggcccgcttg ccaggccgtg tcgccgacct ggatcggcgg cgtcgacgcg attgggccgt
     2101 aaggtttgcc gtcacgccag gccgtaagcg cggcttccag cgaggatcga gcctcgctag
     2161 aagtcggctt gtatcgtgcc tggctggtat cgcagccaat aaacattgcc acgaatggta
     2221 atacggtgat gaacgcacgg aagccgggtc gtagtgcgcg agtcatagtg ttggctcctt
     2281 ttcgcgatca gtaaccgtcg gcagagatga cctcgccgcc gttccgcgtt cccacattcc
     2341 gccaggtttg tacgttgacg gtgcttttga tgaatcgccc cgatgcatcc cccatcagga
     2401 cgttgactcc gccgggatga ttactcgaag g
//
LOCUS       DATAQL010000765         9754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_78910, whole genome shotgun sequence.
ACCESSION   DATAQL010000765 DATAQL010000000
VERSION     DATAQL010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78910"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            573..800
                     /locus_tag="VHS97_13315"
     CDS             573..800
                     /locus_tag="VHS97_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449232.1"
                     /translation="MIISQRLARSRRVGLQALTTEQPAVASLVAGRMIQIALALYLLP
                     ALLVVLLVGCLGLAVLAGSRLLSGPIRAILN"
     gene            1056..2912
                     /locus_tag="VHS97_13320"
     CDS             1056..2912
                     /locus_tag="VHS97_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEX3449233.1"
                     /translation="MTAILGISAFYHDSAAALVVDGRIVAAAQEERFTRLKHDPAFPA
                     RAIEYCLRQAELSSQDLDYVAFYDKPLTKFERLLETYLAYVPSGFRSFRLAMPLWLKD
                     KLHMRRTLRRGLGGDTQARLIFTDHHESHAASAFFPSPFDRAAILTLDGVGEWSTATF
                     GVGEANRVQLVDHIAFPHSLGLLYSAFTYYCGFKVNSGEYKLMGLAPYGRPIYRDLIL
                     ERLMDFKPDGSFWLDMNYFNYCQGLTMTNGRFHRLFGGPPRAPESPLEQRHMDVAASI
                     QVVAEEVVLRIARHVRARTGINQLVLAGGVALNCVANGRLLRAGDFDDLWIQPAAGDA
                     GGALGAALFVWYQLLENPRRPAGQDAQQGSFLGPGFSNAQITRFVSSQSARSQQFTDE
                     LELLDHVAGLLADGKVVGWFQGRMEFGPRALGARSILGDPRSPAMQSTMNLKIKFRES
                     FRPFAPVVLRERAAEWFDLDPKHESPYMLLVADVLEHRRVSVDAEEKKTMTDDPDLRR
                     RVNVVRSEIPAVTHVDYSARLQTVDLERNPRLYRLLRAFDRLTGCPVLINTSFNVRGE
                     PIVCTPEDAYRCFVATDMDVLVMEDFVIAKDAVTRDAGLEAREAYLAQFQLD"
     gene            2986..3402
                     /locus_tag="VHS97_13325"
     CDS             2986..3402
                     /locus_tag="VHS97_13325"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HEX3449234.1"
                     /translation="MHWSDIPFDPPRTTLRQFAGLWLVFFGGMALWQGLARGRVGQAL
                     VLALIAVTIGPLGMVRPDWMRWVYVGWMILAFPIGWTVSQVMLAVMFFGLFTPIGLLF
                     RLVGRDSLHRARSPERSSYWSPKPAPADLRRYFKQF"
     gene            3404..3649
                     /locus_tag="VHS97_13330"
     CDS             3404..3649
                     /locus_tag="VHS97_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEX3449235.1"
                     /translation="MIAAHSYDGVLTMGKSTGEVPNEFEKAAAQSQGESLVSEFWAFL
                     AQNKKWWLLPIIIVMLLLGVLIMLSSTAAAPFIYTLF"
     gene            complement(3749..4495)
                     /gene="gpmA"
                     /locus_tag="VHS97_13335"
     CDS             complement(3749..4495)
                     /gene="gpmA"
                     /locus_tag="VHS97_13335"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX3449236.1"
                     /translation="MIKLVLLRHGESIWNKENLFTGWTDVDLSEQGKAEANQAGELLK
                     AEGFTFDVAFTSVLKRAIRTLWIALDALDLMWIPVEHSWRLNERHYGALQGLNKAQTA
                     AKYGNEQVLVWRRSYDEPPPPLEKSDPRYPGTDPRYRGLSPSEIPLTECLKDTVARFL
                     PYWHQTIVPAIKSGKKVIIAAHGNSLRALVKHLDQMSDHDIVGLNIPTGVPLVYELDK
                     ELKPIRHYYLGDPEAVARAAAAVANQGKAK"
     gene            4666..5850
                     /locus_tag="VHS97_13340"
     CDS             4666..5850
                     /locus_tag="VHS97_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX3449237.1"
                     /translation="MANPFSVLLERSVKPSRLVVGLMSGTSADSIDVAICRLTGQGSE
                     IAVELIFYREYPHDAEVTSQIQRAADLDVRAIAELHVTIGEAFATACLRSLEEAAILV
                     DDVDIIGSHGQTIYHHSGIEGARRATIQLGDGDVIAVRTGCYVVADFRARDVAAGGEG
                     APLSPIADTLLFAGWKPGASRRRAILNLGGIANLTVLDDDPTRVFGFDTGPANAPLDR
                     LARRLSAGALAYDRDGQFARAGRVNEPLLAELLEEDLYLARRPPKSTGFEMYGDAFVA
                     QAALRHGGDDTNLMATLVEFTARTIALGLCQCAQLGPPVEEVIAAGGGVKNPALMERI
                     TALIAPVPLRRSDDLGVPSGAREAMVFAVLADMTLRGMTAFLPPVTGAIAPKLLGKLS
                     FP"
     gene            5992..7455
                     /locus_tag="VHS97_13345"
     CDS             5992..7455
                     /locus_tag="VHS97_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX3449238.1"
                     /translation="MNSIDLIVIALYVGGCTALGAWLGSKSQGLKGYFLAESDIPAWA
                     VMISIVATETSTATFLSVPGVGYQGDFTLLQLPFGYMLGRVVVATVLLPAYFRGQIFT
                     AYQLLRDRFGGPTRTTASVLFLVARSLGDGLRLFLAATVLQHLTGWSAGTAIVAVALI
                     TVVYTFLGGMKAVIWTDVIQFSVYIAGALVALLILVGKLPGGWSQLTEVAGRAGKFRL
                     LDFSPDLTRPYTFWAGLLGGTVLNTATHGADQMMVQRYLAARSQRQAAGALIASGFVI
                     ITQFALFLFIGVSLWVFFGEFPVSGATIRSDERFTYFIIHNLPTGVLGLVIAAIFSAA
                     MGTLAGSLNSSASTIVNDLYRPLTGQSDERHLMRVSRAMTVLWGCVLTAVAFGARRLE
                     DNVVNNALAIASFVSGILLGLFLLGILTRRVGQSAALAGVLAGLVAVSFAKFQTPLAW
                     PWFALVGSLTVYCVGLAASLFLPQQPVESSVEVLADP"
     gene            7491..8408
                     /gene="murQ"
                     /locus_tag="VHS97_13350"
     CDS             7491..8408
                     /gene="murQ"
                     /locus_tag="VHS97_13350"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX3449239.1"
                     /translation="MALEDDLLTESRNPHSESIDTLTALEFVRLMNSEDRKVVAAVEA
                     EAEPIALAIEWAAERLERGGRLVYVGAGTSGRLGVLDAAECPPTFSTPPTMVVGLIAG
                     GAPALTRAVEGAEDDPDSGAADITALNVNANDLVIGIATSGRTPFVLGAMEEARRRGA
                     ATIGVACNRPSLIGKAVDLEIAPLVGPEIISGSTRLKGGTATKMILNMISTGAMVRIG
                     KTLGNWMVDLHPSNEKLRIRSRRILRELVGLDDDRAAQILARCGGHLKRALVVALTAI
                     EPDAAQVLLDAHHGRVRAAVKAAGGRDLP"
     gene            8405..9373
                     /locus_tag="VHS97_13355"
     CDS             8405..9373
                     /locus_tag="VHS97_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX3449240.1"
                     /translation="MTGAGSELSVRLLGVDGGGTATEAWLAAPGGHVLGRGTAGPSNT
                     KAVGPEAARRAIDAAITAAFRAARLSRAPVDVVCLGLAGFDRPEDRQLLAGWADQAQW
                     GHRLVMVNDGDLVVAAGTPEGWGVGVIAGTGSIAVGRGPDGRTARAGGWGHLIGDEGS
                     AYGVVLDALRLVARRWDGREHRPAGRDPLSDRICSILGVTSPAQMVTTIYAPGFDRAR
                     IASLAPEVLAACALAPEIGERLLVPAGAALAEIVRAVARSLEWSSGPLPLAVAGGFLL
                     SATIVRHAMIDNLSRQGYLVHVSPVTEPVIGAIILAERALGAAPTT"
     gene            9559..>9754
                     /locus_tag="VHS97_13360"
     CDS             9559..>9754
                     /locus_tag="VHS97_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449241.1"
                     /translation="MPGKVNSASEEYNSKVGFSRLVIGFIILGVVLRLVRCLLNYPMW
                     CDETMLAANLLDRRWTTLAQP"
BASE COUNT         1706 a         2861 c         3078 g         2109 t
ORIGIN      
        1 gaccgtcaaa atgtaatcaa cgttgttcag ttcagtcaaa ttaaaaatgc gtgttggtca
       61 acgtaggtcc cgtcgacggt agcctcgtgt tgacagatcg caacatacca cctgttcgtt
      121 cgtgcgtgca ctgggccaca aacggcagcg caagaacgcc tacactagct actctactgg
      181 acgatcgtta gtgacaacgt ggccggttta tttccatgct cgatcacatg gatggtattt
      241 cgcggccgaa tattggcgct ttaacgactc aggtagggca cgcacaaccg gcaactccgg
      301 tccaaccagc atttccaaaa aaacaaattc gtaaacgaga cttacctgaa cgccgtttga
      361 tgacctaagt taatgttatg acgagacagt tgaccggaac acagcgaccg agcgtgctca
      421 gaatgttagt gactgaggcg gcgatacctg acatccactc gtgacttcat cacgcatttt
      481 cgagctgagt cgccgggaca cttctcaagt ggcgcacgaa tagcaggcgg cgtgagattc
      541 agaacgaaat tccgcgaaag gggtttacga tcatgatcat ctcgcagcga ttggcgcgct
      601 cgcgacgagt cggtctgcaa gccctaacga cggaacaacc tgcggtggcg agtctcgtgg
      661 cgggccggat gatccagatc gccttggctc tgtacctctt gcctgccctt ttggtcgtgt
      721 tattggtggg gtgtctgggg ttggcggtcc tggcgggcag ccggctgctt tctggtccga
      781 tccgggcaat actcaactag tgaaagcatc agggtcgcag cggctaccgg ccgattagcg
      841 aactctgcat tccaatcacg cctgccctgc cgcctcagtt ttcctggctt ccgattcgct
      901 cgggagatca tgattgccga cgccgacggc aatgagcgtt tcgactctgc gatctgggtc
      961 gcggcatgga aaccctctgg tggcaccggt tatgatgagg ttctccttgc ccgtgtctgg
     1021 aattcaggga cgggatcgaa gacgggtgcc gctcaatgac tgcgatcctg gggatctcgg
     1081 cgttttatca cgacagtgcc gccgcgctcg tggttgatgg gcggattgtt gccgcggccc
     1141 aggaagagcg gtttacgcgg ctcaaacatg atccggcttt tcctgcccgc gcgatcgaat
     1201 actgcctgcg gcaagcggag ctatcgtcgc aggatctgga ttacgtcgca ttttacgaca
     1261 aaccactcac gaagtttgaa cggctgctcg agacctacct ggcatacgtg cccagcggat
     1321 ttcgtagctt tcggctggcg atgccactct ggctcaaaga caagttgcac atgcgccgga
     1381 ctttgcggcg tggtcttggc ggcgataccc aggcgcgctt gattttcacc gaccatcacg
     1441 agagccatgc cgccagtgct tttttcccta gcccattcga tcgcgccgcg atcttgactc
     1501 tggacggagt cggcgagtgg agcacggcta ctttcggagt cggtgaggca aatcgcgtcc
     1561 agctcgtgga ccacatcgcg tttccgcatt cgcttggtct cttgtattcc gcgttcacct
     1621 attactgcgg attcaaggtc aacagcggcg aatacaaatt gatgggattg gctccctacg
     1681 gacggccgat ttaccgcgat ttgatcctcg agcggctgat ggattttaag cccgatggca
     1741 gcttctggct ggatatgaat tatttcaatt actgccaggg cctgacgatg accaacgggc
     1801 ggtttcaccg gctgttcggc ggtccgcccc gcgctcccga gtcgcctctg gagcaacggc
     1861 acatggacgt ggccgccagc atccaggtgg ttgccgagga ggtcgttctg cggatcgccc
     1921 ggcacgtgcg ggcccgaacg ggcatcaatc aactggtcct ggccggcggc gtggcactca
     1981 actgcgtcgc caacggccgc ttgctccgcg cgggcgactt tgacgatctc tggattcagc
     2041 cggctgccgg cgatgcgggc ggcgccctgg gcgcggccct gttcgtctgg tatcaactcc
     2101 tggaaaatcc ccgtcggccc gcgggccaag atgcccagca ggggagcttc ctgggccccg
     2161 gcttttcgaa cgcgcaaatc actcggttcg tgtcgtctca aagcgctaga tcgcagcaat
     2221 tcactgatga gctggagttg ctcgaccacg tggcgggctt actggccgat ggcaaggttg
     2281 tcggctggtt ccagggtcgg atggagtttg ggcccagggc cctgggcgct cggagcattc
     2341 tgggcgatcc ccgctcgccg gccatgcaga gcacgatgaa tcttaagatc aagtttcgcg
     2401 agagctttcg cccgttcgcg ccggtcgtct tgagggagcg ggcagccgaa tggttcgacc
     2461 tggatcccaa gcacgaaagt ccgtacatgc tgctggtggc tgacgtgctc gagcaccggc
     2521 gcgtctccgt ggatgcggag gaaaagaaga ccatgaccga cgaccccgac ctgcggcgcc
     2581 gggtcaacgt tgtccgttcc gagattcccg cggtgacgca cgtcgactat agcgcccggc
     2641 tccagaccgt cgatctagag cgtaatccgc ggctttatcg cttgctccgg gcgttcgacc
     2701 gtctgacggg atgccccgta ctgatcaaca cgagctttaa cgtgcgaggt gagccgatcg
     2761 tgtgcacgcc cgaagacgcc taccgctgtt tcgtggccac cgacatggat gtgctggtga
     2821 tggaagattt tgtgatcgcc aaagacgccg tgacgcgtga cgccggtctg gaagcgcgcg
     2881 aggcttatct tgcccagttc cagctcgatt gactcgatta tgtatgatgt tggcccgagt
     2941 ttatcgtgat catgatatgt tcgcctgatt gagaagaggc cgacgatgca ctggtctgat
     3001 attccctttg atccgccgcg gaccacgctc cgtcagtttg ccgggctctg gctggtcttt
     3061 ttcggtggaa tggcactgtg gcaagggctg gcgcgtggtc gtgttggcca ggcgctcgtt
     3121 ttggccctga ttgctgtaac gatcggtccg ctggggatgg tccggcctga ttggatgcga
     3181 tgggtttatg tgggttggat gattctggcg ttcccgatcg gctggaccgt ctcgcaagtc
     3241 atgctcgcgg tgatgttctt cgggctgttc accccgatcg gattgttgtt tcgcttggtc
     3301 ggccgcgatt cgctccatcg cgctcgttcg cccgagcgga gttcgtactg gtcccccaag
     3361 ccggcaccgg ccgatctacg gcggtatttc aagcagttct aggatgatcg ctgctcactc
     3421 ttacgacggg gtcttgacaa tgggcaagtc cactggcgaa gtaccaaacg agtttgagaa
     3481 agcggccgcc cagtcccagg gcgagagcct ggtgagcgag ttctgggcgt ttctggccca
     3541 gaacaagaag tggtggctgc tgccgatcat catcgtcatg ctgctgctgg gcgtcttgat
     3601 catgctctcc tcgaccgccg cggcgccctt tatatatacg ctgttctgaa ccagggcgac
     3661 tctgcacaaa cccagatttc gcgtggcatt cgctcgtcgc ggcagactgc ctttggcgaa
     3721 ccttgccaca gcttgcagca gcagggggct atttcgcctt gccctgattg gcaacagccg
     3781 ccgccgccct ggcgacagcc tcggggtctc caaggtaata atgacggatc ggcttgagct
     3841 ctttatcgag ctcatagacc aaagggacgc cggtggggat gttcagaccg acgatgtcgt
     3901 ggtcagacat ctggtccagg tgcttgacca gggcgcgcaa actattgccg tgggccgcga
     3961 tgatcacttt cttgccactc ttgatcgcgg gcacgatcgt ctgatgccag taggggagga
     4021 accgcgcgac cgtatccttg agacattcgg tcaagggaat ctccgatggg ctcaacccgc
     4081 ggtagcgcgg gtcggtgccg ggatagcgcg ggtctgattt ttcaagcggc ggcggtggct
     4141 cgtcgtagct gcgacgccat accaacacct gctcgttccc atacttggcc gcggtttgag
     4201 ccttgttcaa gccttgcagc gcgccataat gacgctcgtt gagccgccac gagtgctcga
     4261 cgggaatcca catcaagtcg agtgcatcca gcgcgatcca gagcgtgcgg atcgcccgct
     4321 tcaacaccga cgtgaacgcg acatcaaacg taaacccctc ggccttcagc agctctcctg
     4381 cctggttggc ctcggccttt ccctgctcgg acagatcaac atccgtccag ccagtgaaga
     4441 ggttttcctt gttccagatg ctttcgccat gacgaaggag gacaagcttg atcatcgatc
     4501 ttcctgtatg aacgcgattt tactggttta ccggttggcg acgcggtaga cgtcatcttg
     4561 gcgacatatt ctaccggaac gactcactgg tgcggtggcg atcgggccca catcatggaa
     4621 cgttgggccg aggtccttac attgtcttgg ggagacgaaa ggcggatggc taacccattt
     4681 tcggttcttc tcgagcggag cgtcaagccg tcgcgcctgg ttgtgggcct gatgtcgggg
     4741 acgagcgccg actcgatcga tgtcgcgatt tgccggctga ccggccaggg gagcgagatc
     4801 gcggtcgagc ttatatttta tcgggagtac ccccacgacg ccgaggtgac gagccagatt
     4861 cagagggccg ccgacctaga cgtccgtgcc attgccgagc tacatgtcac gattggcgaa
     4921 gcgtttgcca cggcttgcct gcgatcactc gaggaggcgg cgatcttggt ggacgatgtg
     4981 gatatcatcg gctcgcacgg ccaaacgatt tatcaccaca gtggtatcga gggtgcgcgg
     5041 cgggcgacga ttcagctcgg ggatggcgat gtgatcgcgg tccggacggg ttgctacgtg
     5101 gtcgccgatt tccgtgcccg cgatgtcgcc gccggtggag aaggcgcgcc actttcaccg
     5161 atcgccgaca cgctcttgtt cgcgggctgg aagccaggag catcccggcg gagggcgatc
     5221 ttgaatctgg gcgggatcgc caatctcacg gtactggatg acgatcccac gcgcgttttc
     5281 gggttcgaca ccggacccgc caatgcgccg ctggaccgtt tggcacgcag gctctcagcg
     5341 ggcgcgcttg cgtatgatcg agatggccag tttgctcggg ccggacgagt gaacgagccg
     5401 ctcctcgccg agctgctgga agaggacctt tatctggctc gccgtccacc gaaatcgacc
     5461 gggttcgaga tgtatggcga cgcattcgtc gcccaggccg ctctacgaca tgggggcgac
     5521 gacacaaacc tgatggccac gcttgtcgaa ttcaccgcaa gaacgatcgc gctggggctt
     5581 tgccaatgcg ctcagctcgg gccgcccgtc gaggaggtta tcgcggccgg cggcggagtg
     5641 aagaatccgg ccttgatgga gcgaatcacc gcgctcatcg ccccggttcc attgcggcgg
     5701 tccgatgacc tgggtgttcc ctcgggcgcc cgcgaggcga tggtcttcgc cgtcctggcc
     5761 gatatgactt tacggggcat gaccgccttt cttccccccg tcactggagc catcgcgccc
     5821 aaattgctcg ggaagctctc gtttccttaa gagcacggca cgtgctacgc tgacgggcgc
     5881 ggattcgggt aaatcgaacg attgttggtc gacaccgcag tgcttgtacc aaccatcctt
     5941 tagcctcccg ccctaccgaa accaaaaaca cagttacact tatatcagca gatgaactca
     6001 atagacctga tcgtgatcgc gctctacgtc ggcggttgca ccgcgctggg agcgtggctg
     6061 ggctcgaaat cgcaaggctt gaaggggtat tttctggccg agagcgacat acctgcttgg
     6121 gccgtgatga tctcgattgt ggcgacggag accagtaccg cgacatttct cagcgtgccg
     6181 ggagttggct accagggtga cttcaccctt ttgcagctcc cattcggcta catgctcggg
     6241 cgagtggtgg tcgcgaccgt tctcttgcct gcctattttc ggggccagat ttttacggcc
     6301 tatcaacttc ttcgagatcg gttcggcggg ccgacgcgga cgacggcctc ggttctgttc
     6361 ctggtcgcgc gctcgcttgg ggacggcctg cggctgtttt tggcggcgac ggtgctccag
     6421 cacctgaccg gctggtcggc cggcacggct atagtcgccg tggctcttat caccgtcgtc
     6481 tacacctttc tgggtggaat gaaggcggtg atctggactg acgtcatcca gttcagtgta
     6541 tacatcgctg gtgcgcttgt ggctcttttg atcctggtgg gcaagctgcc gggagggtgg
     6601 tctcagctaa ccgaggtggc cggtcgggca gggaagttcc gactgctgga tttttctccc
     6661 gacttgacca gaccgtacac cttctgggcg ggtctcttgg gagggacggt gctcaatacg
     6721 gcgacccatg gggccgacca gatgatggtt cagcgctacc tggcggcccg ttctcaacgg
     6781 caggcggcag gtgccctgat cgccagtggt ttcgtgatca tcacgcaatt cgcacttttc
     6841 cttttcatcg gtgtgagcct ctgggtgttc ttcggggagt ttcccgtttc gggagccacg
     6901 atccgctcgg atgagcggtt tacctacttc atcattcaca acctgccgac gggtgtgctc
     6961 gggctggtga tcgccgccat cttctcggcc gccatgggga cgctcgccgg ttcgctgaat
     7021 tcgtcggcgt cgacgatcgt caacgacctt taccggccct tgaccggcca gagcgacgag
     7081 aggcacctga tgcgtgtctc gcgtgcgatg accgtgcttt ggggatgcgt tttgacggcg
     7141 gtggcttttg gcgctcgccg gctggaagat aacgtcgtga acaatgcgct ggcgattgcc
     7201 tcgttcgtct ccggcatcct cttgggtctc tttttgctgg ggatcctgac ccggcgggtc
     7261 gggcagtcgg ccgccttggc gggagtactc gcgggccttg tggccgtcag ctttgccaag
     7321 tttcaaacgc cgctggcctg gccctggttc gccctggtcg gttcgctcac cgtctattgc
     7381 gtcgggttgg ccgcgagcct gttcttgcct cagcaaccag tagaatccag cgtcgaagtc
     7441 ctcgccgatc cgtgatgcga tcggctttcc attgtgcggc tgacacgacc atggcacttg
     7501 aagacgacct gttgaccgaa tcacgcaatc cgcattccga gtccattgac acactgaccg
     7561 cactcgagtt cgttcgtctg atgaactcgg aggatcgcaa ggtcgtggca gccgtggagg
     7621 cggaagccga accgatcgca ctggcgattg aatgggcggc cgagcggctg gagcgcggcg
     7681 ggcgcttggt ttacgtcggc gcagggacgt ccggacgtct gggcgtcctc gatgccgcgg
     7741 aatgtccccc cacgttcagc acgcctccca cgatggtcgt cgggttgatc gcgggaggcg
     7801 cgccggcctt gacgcgggcc gtcgagggag ccgaggacga tcccgacagc ggagccgccg
     7861 acatcactgc gctcaacgta aacgcgaatg atctcgtcat aggcatcgcc acatcgggga
     7921 gaacgccctt cgttctcggc gccatggaag aggcgcggcg gcggggcgcg gcgacaatcg
     7981 gcgtagcgtg caaccgtccc agcctgatcg gcaaagccgt cgacctcgag atcgccccgc
     8041 tcgtgggtcc ggagatcatt tcgggatcga cgcgtttgaa ggggggaacg gccacaaaga
     8101 tgattctgaa catgatttca accggagcca tggtgcggat cggcaagacg ctcgggaatt
     8161 ggatggtcga ccttcatccg tccaacgaaa agctgcggat tcgcagccgg cggattctgc
     8221 gggaactggt gggcttggac gacgatcggg ctgcccagat cctggcacgt tgcggaggac
     8281 acctgaaacg ggcgctggtc gtggccctga cggccatcga gcccgacgct gcccaagtgc
     8341 tcttggatgc gcaccatggc cgggtgcggg ccgcggtcaa ggcggcgggt gggagagatc
     8401 tgccatgact ggtgccggat cagaattgtc tgttcggctg ctgggagtcg acggcggagg
     8461 gaccgcgacg gaagcctggc tggccgcgcc cggagggcac gtactgggtc ggggaacggc
     8521 tggaccgtcg aacaccaagg ccgtcggtcc ggaagccgct cgacgggcaa tcgacgcggc
     8581 aattactgcc gcgttccgtg ccgccaggct aagtcgcgcg ccggttgatg tcgtgtgctt
     8641 gggcttggca ggtttcgacc gacctgaaga tcgccagctc ctcgccggct gggccgatca
     8701 agcacagtgg gggcaccggc ttgtgatggt caacgacggc gacctcgtcg tcgcggccgg
     8761 gacgcctgaa ggttggggtg tcggagtgat cgcaggcacc ggctcgatcg ccgtgggccg
     8821 cggcccggat ggacgtaccg cacgcgccgg tggctgggga cacctgattg gcgatgaggg
     8881 aagtgcatac ggcgtcgtgc ttgacgccct gcggctggtg gcccgccgct gggatggtcg
     8941 tgagcaccgt cccgctggcc gcgaccccct ttccgaccgg atctgctcga tactcggcgt
     9001 gacgagcccc gctcagatgg tgacgacgat ctacgcccct gggtttgatc gcgccaggat
     9061 cgcctcgctc gctcccgagg tcctggcagc atgtgcccta gctcccgaga tcggtgagcg
     9121 gctcttggta cccgcggggg cagcgctcgc ggagatcgtc agggccgtgg cgcgctctct
     9181 ggaatggtcg tcaggacccc ttccactagc cgtggccggc ggattcctcc tctccgcaac
     9241 catcgtgcga cacgccatga tcgacaatct cagccggcag ggatatctcg tccatgtatc
     9301 tccggtcacc gagcctgtaa tcggtgcgat catcctcgcc gagcgcgcac tcggcgcggc
     9361 accaacgacg tgacgttgct ccattaatca cttcatcaga tgtcacttaa tttgagtcgc
     9421 cagtcattct tgttactttc ccgtttgctc catttcaacc aactaaaggt aaccaaacat
     9481 catcgttgag ccgatgatag aggcgagatc aaataaaacc gaaaagctgc ggcgcgaccg
     9541 gcacggaggc tcttcggcat gccaggcaag gtcaatagtg cgagcgaaga gtacaattca
     9601 aaggtcggtt tttcacggct tgtgatcggt tttatcattc tgggcgtggt gctgcgcctc
     9661 gtgcgctgcc tgctgaatta cccgatgtgg tgcgatgaga cgatgctggc agcgaacctg
     9721 cttgatcgcc gctggacgac gcttgcacaa ccct
//
LOCUS       DATAQL010000766         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4963735, whole genome shotgun sequence.
ACCESSION   DATAQL010000766 DATAQL010000000
VERSION     DATAQL010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4963735"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2164)
                     /locus_tag="VHS97_13365"
     CDS             complement(71..2164)
                     /locus_tag="VHS97_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3449242.1"
                     /translation="MPTFVGIDLGTTNSAVAYRNAYGRPEVIANREGQNITPSVVYFG
                     ADPPVVGQEAKEWARLGNEEIASFFKPHMGNPLFQLQFQGKAYSATELSALVLRRLKE
                     DAETRLGDKVDRAVITVPAYFADPQRKATIEAGTAAGFQVLRIINEPTAAVLAYGLQK
                     TAVNETVLIYDLGGGTFDVTVARIMPDEIVILATAGDHDLGGKNWDDRIATFLAERFA
                     GETGFDPLDDSVALNEVLVRSEQAKWALSERNSTRITLQLGTERRGFELTRAEFESMT
                     FPLMDRTRRLTEEALAEANLAWPGLDGVLLVGGSTRMPMVRSYVSQMAGKPPRTGVNV
                     DEVVALGAAMQAAIEVGQSIGDAVPRFTLSAGVPQPAKTSAATGRRVTDVMSHSLGTI
                     AVSPDGSAYLNDVVIRRNLPIPARDTKSYIHETHGGANTRLEVYLTQGESLTPLDCTI
                     LGKYVFDDIHPTNAEVTVDVGFSYDSNGVVQVQAVQRDTGHRLPMTVEPVPDDLSWLG
                     RPPETAEAAHELIRVFLLLDVSSSMTGQPLIEAQTAAREFLSKCDLTTMEVGLISFST
                     LVALQSVATSNARRLHAAVHRLEAEGSTNLTDALEMARGQLVSGDRKRYVVILTDGYP
                     DAPQSALEQALAAREQGIEIVAIGTGDADRDYLRRLASSDQASIFARSGELVQAFGHI
                     ARVIAEGGRGLRVLS"
     gene            complement(2247..>2747)
                     /locus_tag="VHS97_13370"
     CDS             complement(2247..>2747)
                     /locus_tag="VHS97_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3449243.1"
                     /translation="TQALDHLEKQLGFTLTGPVRELVNTRIVNVLSKSLGVVARNDQS
                     LDVVYYLLPRNSEVPLERISDFGTDADNQSAVDIRVMSGERDSPDPLDCQEVGVASLT
                     LPERLPAKSPIRVKFAISKDGRLGVSATDLTGGRSIDVEFQTEAVLSAEEVAERSTAL
                     RLMSVS"
BASE COUNT          494 a          852 c          896 g          505 t
ORIGIN      
        1 gtgggtggtg aaaccggcgg cgctgtcgcg gccggcgcgg ccgatgtcgg gcctggtaag
       61 gtggctcggc tcatgataag acgcgcaggc cgcgccctcc ctcggcgatg acgcgggcga
      121 tgtgtccgaa cgcctgaacc agctcaccgc tccgcgcaaa gatcgacgcc tgatcgctgc
      181 tggcgagccg gcgcaggtag tcgcggtcag cgtcaccggt gccaatggcc acgatctcga
      241 tcccttgctc gcgggcggcc agtgcctgtt cgagcgcgct ctggggggca tcgggatagc
      301 catcggtcaa gatcacgaca taccgcttac gatcgccgga gacaagctgg ccgcgtgcca
      361 tctcgagggc gtcggtcagg ttggtgctcc cctcggcctc gagacggtgc acggccgcat
      421 ggagccgccg cgcgttgctc gtcgccacgg attgaagcgc gaccagggtg gaaaacgaaa
      481 tcaggccgac ttccatggtc gtaagatcgc atttcgacaa aaactcgcgg gcggcggtct
      541 gggcctcgat caggggctgg cccgtcatgc tggacgagac gtcgagcagc aagaagacgc
      601 gtatcagctc gtgcgccgcc tccgcggtct cagggggccg accaagccac gaaagatcgt
      661 cgggaactgg ttccacggtc attggcagcc ggtggccggt gtcgcgctga accgcttgaa
      721 cctgtacgac gccattggag tcgtacgaaa agccaacatc gaccgtcacc tcggcgttgg
      781 tcgggtgaat gtcgtcgaag acatacttgc cgaggatcgt gcaatcgagc ggggtcaagc
      841 tctctccctg tgtcagatag acctccagcc gggtattggc gccgccgtga gtttcgtgga
      901 tgtacgactt ggtatcgcgc gccgggatcg gcagattgcg gcggatgacg acgtcgttca
      961 gataagccga gccgtcgggg ctaacggcaa tcgtacctag actgtgcgac atgacgtcgg
     1021 tcacacggcg gccggtcgca gcactggttt tcgccggctg gggcacgcca gccgaaagcg
     1081 tgaatcgcgg cactgcgtcg ccgatgctct ggcctacttc gatcgccgct tgcatcgccg
     1141 cacccagcgc gacgacctcg tcgacgttga ctcccgttcg cggaggttta ccggccatct
     1201 gcgacacgta ggaacggacc atcggcatcc gtgtcgagcc gccgaccaag agcactccgt
     1261 cgagccctgg ccatgccagg tttgcctcgg caagggcttc ctcagtcagc cgccgcgtgc
     1321 ggtccatcaa gggaaaagtc atcgactcga attcggctcg tgtcagctcg aaaccgcgcc
     1381 gctctgtgcc aagctgcagg gtgatccgcg tcgaatttcg ttcggaaaga gcccacttcg
     1441 cctgctcgct gcggaccaaa acttcgttga gcgcgacgga gtcgtcaagt gggtcgaaac
     1501 cggtctcgcc agcgaaccgc tccgccagga acgtggcgat ccggtcgtcc cagttcttac
     1561 cccccagatc gtggtctccc gccgtggcca ggatgacgat ctcatccggc atgatccggg
     1621 ccactgtgac gtcgaacgtg ccaccgccca ggtcgtagat caggacggtc tcgttgacgg
     1681 cggtcttttg tagcccgtat gccagcaccg cagcggtcgg ctcgttaatg atgcgcaaga
     1741 cctgaaaccc ggcagccgtg cccgcctcga tcgtggcctt gcgctggggg tcagcgaaat
     1801 aagccggtac ggtgatcacc gcgcgatcaa ccttgtcccc cagccgcgtc tcggcgtctt
     1861 ctttgagccg gcgcaacacc agggccgaaa gctcggtggc cgagtacgct tttccctgga
     1921 actgaagctg aaacaaggga ttgcccatgt ggggcttgaa aaagctggcg atctcttcgt
     1981 tgccgagccg ggcccattcc ttcgcctcct gaccgaccac gggaggatcc gcgccgaaat
     2041 agacaaccga cggcgtgatg ttttgcccct cgcggttggc gattacctcg ggacggccgt
     2101 acgcattgcg gtaggccacc gccgagttag tcgttcccag gtcgattccg acgaaggtgg
     2161 gcatcagctt aaagcgcctt ggtgttggcc gggcttgagc gtgacgaata ggcgaagtgc
     2221 cgtcgaaaca gggagattgc tgaaattcac gacacgctca tgagccgcag ggccgtcgag
     2281 cgttctgcga cctcctcggc ggagaggacg gcctcggtct ggaactcgac gtcgatcgag
     2341 cggcctccag tgagatcggt cgccgataca cccagcctgc cgtctttgct gatggcgaac
     2401 ttgacgcgga tggggctctt ggccggcagc cgctcgggca acgtgagcga ggcgacgccg
     2461 acctcctggc agtcgagcgg gtcgggactg tcgcgttcgc ccgacatgac tcgaatatcg
     2521 acagccgact ggttgtcggc gtcggtaccg aagtcactga tgcgctccag cggcacctcg
     2581 gaattgcgcg gcaggagata atagacgacg tcgaggcttt gatcgttgcg cgcaacgacg
     2641 cccaggctct tggagagaac attgacgatg cgcgtgttga caagttcgcg taccggccca
     2701 gtcagggtga aaccgagctg tttctccaga tggtcgagcg cctgggt
//
LOCUS       DATAQL010000767         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4505932, whole genome shotgun sequence.
ACCESSION   DATAQL010000767 DATAQL010000000
VERSION     DATAQL010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4505932"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2907
                     /locus_tag="VHS97_13375"
     CDS             <1..2907
                     /locus_tag="VHS97_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449244.1"
                     /translation="LSPNPEDLPPNIEPLPDADGSIPVPSLPRTGPGQGGNAAPGAGG
                     QFPVVPISPGTQRTTSLFPRSGRQLQIVQYPATAEGVVTYVCRGGINLVTKSPKFGTI
                     DIEADEAVIWRGPDREKGAPAIRPNGETFVDDSSVPMEVYMEGNVVLRQDENKVAGKG
                     DQRTARAPRLYYDFLFDRFLAPNAEIDLFAPTFLSPIKFKSPRIEQFHRPILLPNGTS
                     VLSDQPEIRMEPAVTTGSRFPNPGYKVTNRSIDLTRHSRPLTNPNTGKEVKNPKNPNA
                     TPEEDIWRVDARQNFYFAGPLPVWYWPHIVTDIEDMEPPLRMIGYSNNSYFGEMFMAE
                     INGFRLIQTRRPKWIDLWNVDVDYLSFRTKNFPALGSEIGWFGTDLIRDLNDPYHEDR
                     NPPDHLTKSYFGYFDIWGLQDAGIDVLGTGPAIVTNGPPGAGKAGFQRSAVPPFQSER
                     GRLNLRHMQRFLPDDEEHEFEDLRLQLEVAYASDRNFIEEYYKRLFDTGLDQETLAYG
                     VWQKDNQFASIWTEANLQNWYTDTQWLPRVDYYRLGDSFGNNLFNYYTHSGLDYATIH
                     TDVMVNNPNLFAFIPFDPISNTTGTFSSGRFYTNHELDMPLNFGNVVRLVPYVQGQAV
                     GWSDQLGGGALGHLPTGAMGRIWGAAGARAEVTAWKQYPGVENELLNVHGLNNKISIF
                     ADARAAYSNQQLNKIAVQDDLDDNTYEYVRRYGALTNFVGGILPMPYDPRHLILRQTL
                     SPITDTTDVQATINTIQLGVHQRLQTKRGPVGKRRIVDYMTLDASTTYFPTANRDNFG
                     TPWGQAMYNYQWYIGDRTSIVSSGWFDFFKLVGSSPLPSITGYNPNGLNIITTGVSIS
                     RPPRANVFLGYSIIDTGPIKTSALNVSLSYWLSPKWYGTFSESYDFGDAISLGSMFSF
                     TRIGADYLMTIGLTVDPQRKNTQFAFQITPRLGTGLGTSANTSGTVDTRFAPTQ"
     gene            2938..3453
                     /locus_tag="VHS97_13380"
     CDS             2938..3453
                     /locus_tag="VHS97_13380"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX3449245.1"
                     /translation="MNDERRPSEIGLATPAPDIDSAHMLQPVPGYDEHALDFDVSSDQ
                     TAHADRRNTRSKLAAGWRGIKSAVRGDSSFFAHGYRGTLIVITAALLGIDQRGWCLLV
                     LGACLVLIAELAHSSIDTLARAIGDPEEPRLKTAREIAAGGVLVAAVGSAAVTIIVLS
                     WKFAELLGSNP"
     gene            complement(3601..>4350)
                     /locus_tag="VHS97_13385"
     CDS             complement(3601..>4350)
                     /locus_tag="VHS97_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3449246.1"
                     /translation="SQEFKETADAIRKSGKAKFVGFSSHHKERADLIQAAADGGIVDA
                     IMLQYTPWLDKDSPLNKALDACHKKGIGLISMKQIAGKFPSGETRNMLEEIVMRAPVL
                     AEKKLSPYQGLLHAIWTDERISGSCVSIKTTDQLREDSDAARRYQPLTTAQIGQLRDA
                     ALAFGPTLCADCDGRCSIAAGTNAELGNLTRFLTYHEHHGIRQEARRQYAALAQEARD
                     WSGADLEAARAACPNKLDFAKLLPEVDRHLA"
BASE COUNT          880 a         1357 c         1249 g          864 t
ORIGIN      
        1 ctgtcgccca atcctgaaga cctgccgcca aacatcgagc cactgccgga tgcggacgga
       61 tcgattcccg ttccctcgct tccccgaacg gggcctgggc aagggggcaa cgcggcgcct
      121 ggagcgggcg ggcaattccc cgtcgttccc atcagtccgg gaacccagcg tacgacctca
      181 ttattccccc gtagcggccg gcagctccag atcgtgcaat atccggccac tgctgagggt
      241 gtcgtcacat acgtctgccg gggtggcatc aatctcgtca cgaagtcacc caagtttggc
      301 acgatcgata tcgaggcgga cgaggcagtg atatggcggg ggcctgaccg agaaaaaggg
      361 gcacctgcga ttcgcccgaa cggcgagacg ttcgtcgacg attccagtgt tccgatggaa
      421 gtgtacatgg aaggcaatgt ggtgctgcgc caggacgaga acaaggttgc cggcaagggg
      481 gatcagcgga ccgcgcgcgc accccggttg tattacgact ttctcttcga ccgcttcctg
      541 gcgcctaatg ccgaaatcga cctattcgca ccgacgttcc tctcgcccat caagttcaag
      601 tcgcccagga tcgagcagtt tcaccgcccg attctgttgc cgaacgggac gtctgtcctc
      661 tccgaccagc ccgagattcg catggagccc gcggtcacga ccggaagccg atttcccaat
      721 ccgggctata aagtcacgaa ccgctccatc gacctgacac ggcattcgcg tccactgacc
      781 aacccgaata ccggcaagga agtcaagaat cccaagaatc ccaacgcaac cccggaggaa
      841 gacatctggc gggtcgacgc tcgccagaac ttttacttcg ccgggccctt accggtatgg
      901 tattggccgc acatcgtcac cgacatcgaa gatatggagc cgcctctgcg catgatcggc
      961 tatagcaaca acagttactt cggcgagatg ttcatggccg aaatcaacgg atttcggctc
     1021 atacagacgc gccggcccaa gtggattgat ctatggaatg tcgatgtcga ttacctgagt
     1081 ttccgcacca agaactttcc cgcgctggga agcgagatcg gctggttcgg aaccgacctc
     1141 attcgcgact tgaacgaccc ctaccatgaa gatcgcaatc cccccgacca tctcaccaag
     1201 agttatttcg gttatttcga tatctggggt ctccaggatg cgggcatcga cgttctcggt
     1261 actggaccgg cgatcgtcac gaacgggccc ccgggggccg gcaaagccgg gtttcagcgc
     1321 agcgctgtcc caccgttcca gtcggaacgc ggccggctca acctccgtca catgcaacgg
     1381 tttctcccgg atgacgaaga acacgagttc gaggatctgc ggctccagct cgaagtcgcc
     1441 tacgcatctg atcgcaactt tatcgaggaa tactataagc ggctcttcga cacagggctt
     1501 gaccaggaaa ccttggccta tggcgtgtgg cagaaggaca accagttcgc aagcatttgg
     1561 accgaggcaa accttcaaaa ctggtatacc gacacgcagt ggctaccacg cgtcgattac
     1621 tatcgcctgg gcgattcgtt tgggaacaac ctgttcaatt actacaccca ttcgggattg
     1681 gactatgcca cgatccatac cgacgtcatg gtgaataatc ccaatctctt cgcgttcatt
     1741 ccctttgacc cgatctcgaa cactacggga acgttttcgt cgggccggtt ctataccaac
     1801 cacgagctcg acatgccgct caacttcggc aacgtggtgc ggctcgtgcc ctacgtgcag
     1861 ggtcaggcag ttggatggtc ggaccagctt ggcgggggag cgctcgggca tcttcccacc
     1921 ggtgcgatgg gccgcatttg gggcgcggca ggcgcccgag ccgaggtgac cgcctggaag
     1981 caataccccg gcgtcgaaaa cgagctcttg aacgtccacg ggctcaacaa caagatcagc
     2041 atcttcgccg atgcccgcgc cgcgtactcg aaccaacaac tcaacaaaat cgccgtacag
     2101 gacgaccttg acgacaacac atacgaatac gtgcgccggt acggggccct caccaatttc
     2161 gtcggcggga tcttaccaat gccgtacgac ccgcggcacc tgatcttgcg ccagacattg
     2221 tcgccgatca ccgatacaac cgacgttcaa gcgacgatca acaccattca acttggggtt
     2281 catcaacggc ttcaaaccaa gcgggggccc gtgggcaagc gccggatcgt cgactacatg
     2341 acgctcgatg cgtcgaccac ctatttcccc acggccaacc gcgacaactt cgggacgccc
     2401 tggggacagg cgatgtacaa ctaccagtgg tacatcggtg accgaaccag catagtttcg
     2461 tccggctggt ttgacttctt caagctggtg ggcagttcac cattaccgag cattacaggg
     2521 tacaacccca atggcctcaa catcatcacg acgggcgttt cgatcagccg gccaccgcgc
     2581 gccaatgttt tcctgggcta ttcgatcatc gacaccgggc cgatcaagac ctcggctttg
     2641 aacgtatcac tcagctattg gttgagcccg aaatggtatg gtacgttctc ggaatcttac
     2701 gactttggcg atgcgatctc gctgggatcg atgttttcat tcacacgcat cggagccgat
     2761 tacttgatga ctatcgggct gacggttgac ccgcagcgaa aaaacaccca gttcgccttt
     2821 caaatcacgc cgcgtttggg tactggattg gggacctcgg ctaacacgag cggcaccgtt
     2881 gatactcggt ttgctcccac gcagtgacct gacacgaagg gcgggtagcc tacgatcatg
     2941 aacgacgaaa gaaggccaag cgagatcggt ctggctacgc ccgcgcccga tatcgattcg
     3001 gcccacatgt tgcagccggt ccctggctac gatgagcacg cgcttgattt cgacgtttcc
     3061 tcggaccaga ccgcccacgc cgatcgacgt aatacgcgca gcaagcttgc cgcgggctgg
     3121 cgcggtatca agtcggctgt ccgcggtgat tcgagctttt ttgcccacgg ctaccgcggc
     3181 accctgatcg tcatcaccgc ggcgttactg ggcatcgatc aacggggatg gtgcctcctg
     3241 gtcttaggtg cctgcctggt cttgatcgcc gaactggccc acagctcgat agacacactg
     3301 gctcgcgcca tcggcgaccc cgaagagccg cggctcaaga ccgcgcgcga gatcgccgcc
     3361 ggtggcgtac tggtcgccgc cgtcggctcg gccgccgtga cgatcatcgt tctgtcctgg
     3421 aaattcgccg aactcctcgg gagcaatccg tgacgattga cttgccccga gcctctttcc
     3481 gtcatttcgc ctccaaatga ggccgccttc agagagcgac gatgcgagca aaggacgacc
     3541 ttttctgaag ctcgctgacc ggcaggaggg gccagtcgcc cccccgccga tccgaatcac
     3601 tcaggcgagg tgccggtcga cctcgggcag cagcttggcg aaatccagct tgttggggca
     3661 cgcggctcgt gcggcctcga ggtcggcgcc cgaccagtcg cgggcctctt gagcgagggc
     3721 agcgtactgg cggcgggcct cttggcggat gccgtggtgt tcatggtagg tgaggaagcg
     3781 tgtcaggttg ccgagctcgg cgttggtgcc ggccgcgatc gagcagcggc cgtcgcaatc
     3841 ggcgcagaga gtcggcccga atgccagagc ggcgtcgcgt agctgaccga tctgggcggt
     3901 cgtcagcggc tggtagcggc gggccgcgtc ggaatcctcg cggagctggt cggtcgtctt
     3961 gattgacacg caagagccac tgatccgctc gtcggtccag atcgcatgca gcagcccttg
     4021 gtacgggctc agcttcttct ccgctagcac gggcgcccgc atgacgattt cctcgagcat
     4081 gttcctcgtt tcaccggagg gaaacttgcc ggcaatctgc ttcatcgaaa tcaggccgat
     4141 gcccttttta tggcacgcgt cgagggcctt gttcaacggc gagtctttat ctagccaggg
     4201 agtgtactga agcatgatcg cgtcgacgat tcctccgtca gccgccgcct ggatcaagtc
     4261 ggcgcgctcc ttatgatggc tcgagaaacc gacgaatttt gctttgccgg acttacggat
     4321 cgcgtcggcc gtctctttga attcctgaga
//
LOCUS       DATAQL010000768         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4201973, whole genome shotgun sequence.
ACCESSION   DATAQL010000768 DATAQL010000000
VERSION     DATAQL010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4201973"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1593)
                     /locus_tag="VHS97_13390"
     CDS             complement(187..1593)
                     /locus_tag="VHS97_13390"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449247.1"
                     /translation="MASSNIMNQDSEPPRRGLHHWLDALGLGRPELRAWAMYDWANSA
                     MACVVITAVFPIYYEQVACAGIKQEDASGRFALATTIGMVIIAVIAPLLGTIADFNGA
                     KKRMLAGFLTLGLTSVAGMFFIYTGDWILASILFILANIGANGTYVFYDALLPHIASD
                     DEIDRVSTAGYALGYVGGGTLLAVNLAWILKPQWFGLPSGDELSAAQATLPTRLSFLS
                     VAIWWLVFSIPLLLRVREPAPRGGLTTDGTIRKLPIREAVDRLVDTGRSLNRYRQALV
                     MLLAFLIYNDGIGTIMRMATIYATEIRIPKEFTIAAIVIVQFVGIPCAFLFGALAGWI
                     GAKRSIGIGLFIYAMICVVGYFMTTGTHFLMLAILVGLVQGGTQALSRSLFASLIPRD
                     KSGEFFGFFGVAEKFAGIFGPAIFFVINWLSGSSRGAIVSIIGFFVVGGLLLTQVDVA
                     EGQRLARAEEAEALAGNM"
     gene            1794..2879
                     /locus_tag="VHS97_13395"
     CDS             1794..2879
                     /locus_tag="VHS97_13395"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX3449248.1"
                     /translation="MRTIIDGHLDLSWNALSWDRDITLELDRLNQGERGMDDHPSRGR
                     ATTTLPEMRRGAIAACQATLLARAKSGSRIHGGTSRLGLDFANQEIASATARGQLAYY
                     QLLERRGLLRMIRTAGELDAHWANWASNSSASPIGYILAMEGADPIVDIGHAATWWEL
                     GLRSVNLAHYGKSRYAVGTGDDGPLTPDGIELLKEFAVLGMILDATHLSDTSFSQALD
                     RFDGPVLASHNNCRALVPHQRQFSDEQIERLMERGAVIGVALDAWMLAPGWVRGQTSR
                     EVVGIESVADHIDHICQLAGNANHAAIGSDLDGGFGTEQSPSGLDRISDLQKLDAILA
                     GRGYSPAGVDDIFHGNWLRFFRQHLPR"
BASE COUNT          755 a         1043 c         1046 g          701 t
ORIGIN      
        1 tcggacacgc tggtctggct caaccagagt tcctcgagat tcggaagtcg cttcagctgt
       61 tctaatccct tgaaagctgg cattctggaa tttgtgatca ccgttcatag gatgcgcgct
      121 cggcggagag atatcacaga ggcaggctct tgcaccatat ttgtgcttgg agagcgaggt
      181 ccatcgtcac atgttgcccg caagcgcctc ggcctcctcg gcgcgcgcca gccgctggcc
      241 ttcggcgacg tcgacctggg tcaagagcag accgccaacg acgaagaatc cgatgatcga
      301 cacgatcgca cctcggctcg atccgctcag ccaattgatc acaaagaaga tcgccggccc
      361 aaagatgccg gcgaacttct cggcgactcc gaaaaagccg aagaattcgc ccgacttgtc
      421 gcgcggaatc aagctggcga agagcgagcg gctcagggct tgcgttcccc cctgcaccag
      481 gcccaccagg atcgccagca tcagaaaatg cgtgccggtc gtcatgaaat aaccgactac
      541 gcagatcatg gcataaataa acaagccaat cccgatcgaa cgcttggcgc cgatccagcc
      601 ggcgagtgcg ccgaacagga acgcacaggg gatccccacg aactggacaa ttacgatggc
      661 cgcgattgtg aactccttgg gaatgcgaat ctccgtcgca taaatcgttg ccatgcgcat
      721 gatggtgccg attccatcgt tatagatcag aaacgcgagt aacatcacga gcgcctggcg
      781 atagcgattt aatgatcgcc cggtatcgac taagcgatcg accgcttcgc ggatgggcag
      841 tttccggatc gtaccgtcgg tggtaagccc gccccgcggc gccggctcgc gtacgcgcaa
      901 gagcagaggt atcgaaaaga ccagccacca gatggcgacc gagaggaaac tcaaccgagt
      961 cggcagagtg gcctgcgcgg ccgacagttc gtctcccgac ggcagcccga accattgcgg
     1021 cttcaagatc caggccagat tgacggccag cagcgtgcct ccgccgacat aaccaagcgc
     1081 ataaccggcc gtcgagacgc ggtcaatttc gtcgtcactg gcgatatgag gtaaaagggc
     1141 atcgtaaaac acgtaggtgc cattggcccc gatgttggcc aggatgaaca atatcgaagc
     1201 cagaatccaa tcgccggtgt agataaaaaa cattccggct acggatgtta gccccaaggt
     1261 cagaaagccg gcgagcatcc gctttttggc gccattaaaa tcggcgatcg ttccgagcaa
     1321 gggggcgatc acggcgatta tgaccatacc gatcgtcgtc gccagggcga atcgccccga
     1381 cgcgtcttcc tgtttgatcc ccgcgcacgc gacttgttcg tagtagattg gaaaaacggc
     1441 ggtgatgacg acgcacgcca tagccgaatt ggcccagtcg tacatcgccc aggcgcggag
     1501 ttccggacgg ccgagaccca gcgcatccag ccagtgatga aggccacgcc gcggcggctc
     1561 ggaatcctga ttcatgatgt tcgagctcgc catcgacgtc cctccatgac ctgactctcg
     1621 gcctaaccgt ggatccaaga gcggtatcgg gctcttgaca tatttccccc ggttgtgaga
     1681 accgcggcga atggggtata tcataacggg ccgactggca gatgaaacgc cgatcagcgt
     1741 aatctccggc tgcctgaaac ataaaccaag gattgcgagg catcctttca ctcatgagaa
     1801 cgatcattga cggtcatctg gatctgtcgt ggaacgccct ttcctgggac cgagacatca
     1861 cactcgagct ggaccgcttg aatcaaggtg agcgagggat ggacgatcac ccttcgcgcg
     1921 ggcgtgctac gacgacgctg cccgagatga ggcgcggtgc cattgcggcc tgccaggcaa
     1981 ccctgctggc gcgggccaag tccgggtcgc ggattcacgg tggaaccagc cgcctgggcc
     2041 tcgactttgc caatcaggag atcgcgtcgg cgacagcgcg cgggcaactt gcttattacc
     2101 agttgctcga gcgccgaggc ttgttgagga tgatccgcac ggccggcgag ctggatgccc
     2161 actgggcgaa ctgggccagc aactcgtccg cttccccgat tggctacatt ctcgccatgg
     2221 aaggggcgga tccaatagtc gacatcggtc acgccgcaac gtggtgggag ctcggcttgc
     2281 ggtcggtaaa cctggctcat tacggcaaga gccgctacgc ggtcgggacc ggtgatgacg
     2341 gccccctcac acccgatggt atcgagctct tgaaagagtt tgccgtgctg ggaatgatcc
     2401 tcgacgcgac gcatctttcg gataccagct tctcccaggc gctcgatcgc tttgacggcc
     2461 cggtcttggc gagccataac aactgccggg ccctggtacc gcatcagcgg caattcagtg
     2521 acgagcagat cgagcggctg atggagcgcg gtgctgtcat cggcgtggcg ctcgacgcct
     2581 ggatgctggc tccgggctgg gtccggggcc aaacttcgcg cgaggtcgtc ggaattgaat
     2641 ccgtggccga ccacatcgac catatttgtc agctcgcagg caatgcaaac cacgcagcca
     2701 ttggcagtga tctggacggc ggttttggaa ccgaacagtc cccgtccggt ctcgatcgca
     2761 tctcggacct gcaaaagctc gacgcgatcc tggccggtcg aggatattca cccgcagggg
     2821 ttgacgacat cttccacggc aactggctgc ggttctttcg acagcacctg ccgcgctgat
     2881 tctggaaggc ccgatctcaa aaagcggcga tactcgctcg ctcatgacga ccgtgagaat
     2941 ttcccaaaca cacgatgatg tgtgcctttc gttcgatgac ctgttattac ggcttacgtg
     3001 acgccgctca gccggctgcc cgataaatcg gcgtagacta tattaaagat gcattacgtg
     3061 actgtacgcc cgttgcgatc ttgaggaccg catctcgcgc gggcaaatcc aattcaagga
     3121 tcccccgata gttcagacga ccatgttggt gtttttcttc agggtttgag ttttttgtgt
     3181 tttcgtactt tcgtgcgatc gtgatttgtt tgggtggacc atcatgagcc atgacttcga
     3241 gccgctttcc gccagagtca atgtcgcttg gtcgtccgcc gggccttgat gccggcttac
     3301 ttggattcgg ctagacgcct tttgggggcg caccccattc tggaagggtg ctgtcacgca
     3361 tggtaaaatt acggataata ccaaggttga tggtggagtc agtgagacgt gtcgagaggg
     3421 ccgcgccggc cggcaggaaa tagcgctttg gacgacgtga cgacgcaggc gtcccagtgg
     3481 tgcgaacagg ctcggcggtt gtgcgaagcc tccgaaaacc ctaaccccca gtgcctgccg
     3541 attct
//
LOCUS       DATAQL010000769         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1300092, whole genome shotgun sequence.
ACCESSION   DATAQL010000769 DATAQL010000000
VERSION     DATAQL010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300092"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHS97_13400"
     CDS             <1..223
                     /locus_tag="VHS97_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449249.1"
                     /translation="NGIPDVAVGAMRRLSPIRWPAWLRGSSAPHSRPLAQVHPKRWQE
                     GRRGIPRSLRPTCLAALRVDLGFFHVGKP"
     gene            complement(205..2091)
                     /locus_tag="VHS97_13405"
     CDS             complement(205..2091)
                     /locus_tag="VHS97_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX3449250.1"
                     /translation="MASSRRRGTLVDYLVAAIEPALIITMVGSLMFFLLDMWYDGPVI
                     DRLRWILFWFVLGIVLITRVSMKIGSNLALGYGVALGGAVALVASVLAGFQPALLVIM
                     GVVWWATHRLTFDCTLLDEDQDAGIGLLQETGLDPESMAEPIPSTPSPTDDDPAAMDA
                     SLLPHHPWWKPGKQDSEEARRPNAPGTWLIYFTLASLPLFALGQWLVPAVEEERRTGL
                     FLYFLAYISSAMGLLLATSFLNLRRYLRQRKLTMPGAMTATWLSTGAIVIVGLTLLAA
                     ALPLPGLGWNVIRGSTPASSDLRASNNAVLKDSGVQGEGAPSEGPAATKGARPPASAG
                     KEKGSGQTNDPNAAQQTAGKGRSGGKNAPGNSKSGAPRGKSAPSKGGAQGKEGSPQEN
                     KGDSGNQNKGKDGQAEKQAEPSKNADEKSSPDDKNAADQDNEKSSQENQNSNDGSSPP
                     SRLPSVPFAAPWWLRALFVAAGVAAVIYGVLRYGLTLLDALRDLLASLFGGLFIQQPK
                     KRAKAPEPPPGEHAPPRRPFASFPNPFETGLDQRFTPNDLVVYSFEALEAWASEHDLA
                     RSPNETPSEFVRRVGEARADLRQDATRVVGYFVTIVYGQKGFQSDVLAPLRQFWRALQ
                     GLPT"
     gene            complement(2113..3441)
                     /locus_tag="VHS97_13410"
     CDS             complement(2113..3441)
                     /locus_tag="VHS97_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3449251.1"
                     /translation="MPWFLAIVIVLVIAVVFDLGLLACAMMALLGLMLISRFLARSWI
                     TNLKATRECNRVEAEVGQTIAFTVTVKNRGQLPVAWVLIEDMLPRGAISSRPPRIVVK
                     GQRLAVAMLGSRGRKSLNYQLHFIRRGYYQVGPLVLETGDLFGLHRRFRIVTEPVYIM
                     VYPKVVPLPGYDIASRRPIGEIRLTHRLFEDPTRIAGVRYYQPGDPLNRVNWAATART
                     GVLHSKIYDASTIAGATIVLDFHQGSYPVRNEPARSELAVTTALALANAVCLMGQPIG
                     LVTNGRDAADRIRLRDEIAREHAGPQMNRAAIHQLAATRERDDRLRPIIVPPGRGAES
                     FSSIREVLARVELTDGLTFDRLLIEAEPRLPRDATVVAILGNVTPEMALSLGHLKRQG
                     FAVNVVLLAFDEVERIDNAGPLLAQGLPVRSLSDEDELYGFCELQLVTPL"
BASE COUNT          666 a         1116 c         1073 g          638 t
ORIGIN      
        1 gaatggcatt cccgacgtgg ccgtgggtgc tatgcgccgt ctgtcgccga tccgctggcc
       61 ggcgtggttg cgcggcagta gcgcaccgca ctctcgaccc ttggctcagg tccacccgaa
      121 gcgctggcaa gagggccgtc gaggaattcc tcggtcgcta cggccgacgt gtcttgcggc
      181 gcttcgggtg gacttgggat tctttcacgt gggcaagcct tgaagcgccc tccagaactg
      241 tcgcagcggt gccaagacgt ccgactgaaa gcctttctgg ccatacacga tcgtcacgaa
      301 gtagcccacc acgcgcgttg cgtcctggcg caagtcggcc cgcgcttctc ccacacggcg
      361 gacgaattca ctcggcgtct cgttgggcga tcgggcgaga tcatgctctg aagcccaggc
      421 ctctagtgct tcgaaactgt agacaacaag gtcatttggc gtgaaacgct ggtccaggcc
      481 ggtttcgaaa gggttgggaa acgaagcgaa gggtcgacgt ggtggcgcgt gctcaccggg
      541 cgggggctcg ggagccttgg cacgtttctt gggctgctga ataaacaaac cgccaaacag
      601 cgaggcaagc aagtcccgca gggcatccag caaggtcaac ccgtagcgaa gcacgccgta
      661 aatcacggcc gcgactcccg cggctacgaa caaggcccgt aaccaccacg gagccgcaaa
      721 cggcaccgag ggcagccgcg acggcggact ggacccgtcg ttcgagtttt gattttcttg
      781 agacgatttc tcgttatctt gatcggccgc attcttgtcg tccgggctcg atttttcgtc
      841 cgcattcttg ctgggctcgg cttgtttttc cgcctggccg tccttgccct tgttttgatt
      901 tcctgaatca cccttattct cttggggtga cccctctttg ccctgcgcac cccctttcga
      961 aggcgccgac ttaccccgcg gtgcgccgct cttggaattc ccgggggcgt tcttgccgcc
     1021 ggaacgccct ttgccggccg tctgttgcgc ggcgttggga tcgttggtct ggccggaacc
     1081 cttttccttg ccggctgacg ccggcggcct tgcacccttc gtcgccgccg gtccctcgct
     1141 cggcgctccc tccccttgca cgccactatc cttgagcact gcattattcg aggcacggag
     1201 gtcgctcgac gcaggcgtcg aaccgcggat cacgttccag cccagacctg gtaacggcaa
     1261 tgccgcggcc agcaaagtca ggccgacgat cacgatggcg cccgtcgata accaggttgc
     1321 tgtcattgct ccaggcattg tgagcttgcg ctgccggaga taacgccgga gattgagaaa
     1381 acttgtcgcc agcagcagtc ccatggcgct ggagatgtag gccaggaagt aaagaaacaa
     1441 ccccgtccgt cgctcttcct cgacggccgg caccaaccat tggcccagcg cgaagagcgg
     1501 aagcgaggcc agtgtgaaat agatcagcca ggttccaggc gcgttcggcc ggcgggcctc
     1561 ttcggagtct tgcttgcccg gcttccacca gggatggtgc ggtaaaagtg acgcgtccat
     1621 cgccgccggg tcatcatcag tcggggaagg ggtcgaagga atcggttcgg ccatggactc
     1681 gggatcgagg ccggtctcct gcagaaggcc gatccccgca tcctgatcct cgtcgagcag
     1741 agtgcagtcg aaggtaagcc tgtgcgttgc ccaccaaact acgcccatga tcaccagaag
     1801 ggcaggctga aatcccgcca gcaccgacgc caccaacgcg accgcaccgc ccagcgccac
     1861 gccgtaaccc agggccaggt tgctgccgat cttcatgctc acccgggtga tcagcacgat
     1921 tcccagcacg aaccagaaca agatccagcg cagccggtcg atgactggcc cgtcgtacca
     1981 catgtcgagc agaaagaaca tcaagctgcc gaccatggtg atgatcagtg ccggctcgat
     2041 cgcggcgacc aggtaatcga ccaatgtgcc acggcgtcgg ctgctggcca tcggggaatg
     2101 ctccgacaat tctcaaagtg gggtcacgag ctgaagctcg cagaaaccgt acaattcatc
     2161 ctcatccgac agcgaccgaa caggaagtcc ctgggccaag agtgggccgg cattgtcgat
     2221 gcgttcaacc tcgtcaaaag caagaagcac aacattgacc gcgaacccct ggcgcttgag
     2281 atgtccaagc gagagggcca tctcgggtgt gacgttcccc agtatcgcca cgaccgtcgc
     2341 gtcgcgaggc agccgcggct cggcttcgat caagagccgg tcgaaagtca ggccgtcggt
     2401 cagctcgaca cgggccagga cctcgcgaat actggaaaag ctttccgcgc cgcggcccgg
     2461 cggcacgatg atgggacgga gccggtcgtc gcgctcgcgc gttgcggcga gctgatggat
     2521 cgccgcccga ttcatctggg gtcctgcgtg ctccctggca atctcatcgc ggagccggat
     2581 tcgatcggcg gcatcgcgtc cattggtgac cagtccaatg ggctggccca tcaggcagac
     2641 cgcgttggcg agtgccagcg ccgtggtcac ggccagctca gatcgcgccg gctcgttccg
     2701 cacggggtag ctgccctggt ggaaatcgag cacgatcgtc gcgcctgcga tcgtcgaggc
     2761 atcataaatc ttgctatgca aaaccccggt gcgagccgtc gctgcccagt tgacccggtt
     2821 gagcggatcg ccaggctggt agtaccgtac acccgcgatc cgggtcggat cctcgaacag
     2881 acggtgagtg aggcggatct cgccgatcgg ccgccgcgat gcgatatcgt agccagggag
     2941 tggcacaacc ttgggataaa ccatgatgta gaccggctcg gtcacgatcc ggaaccgccg
     3001 gtgcaaaccg aagaggtcgc cggtctcgag cacgagcggc ccgacctgat aatagccgcg
     3061 cctgatgaaa tggagctgat aattcagcga cttgcgcccg cgcgacccga gcatggccac
     3121 agccagacgt tgccctttca ccacgatgcg cggaggccgt gagctgattg ccccgcgcgg
     3181 cagcatgtcc tcgatcaata cccacgccac cggaagctgg ccgcggtttt tcaccgtcac
     3241 cgtgaaggcg attgtctgcc cgacttcggc ttcgacccgg ttgcattccc gggtcgcctt
     3301 gaggttggtg atccacgacc gagcgaggaa gcggctgatc agcatcaatc ccagcaaagc
     3361 catcatggcg caagccagca gcccgaggtc gaagacgacc gcgatgacca gaacgatcac
     3421 gattgccaga aaccatggca tcaggccatg accctcgaat tgctcgtcgg gacggggatc
     3481 gtgtcgagta gct
//
LOCUS       DATAQL010000770         4914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1453399, whole genome shotgun sequence.
ACCESSION   DATAQL010000770 DATAQL010000000
VERSION     DATAQL010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453399"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            807..1955
                     /locus_tag="VHS97_13415"
     CDS             807..1955
                     /locus_tag="VHS97_13415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX3449252.1"
                     /translation="MDDVPAGPASPWPRRPALSVVVPVRNGGRDFERCLQRLRDSQVT
                     EFELIVVDDGSTDDTATLAKTAGATVVHHPYPQGPAAARNLGARVATAPFIFFLDADV
                     AIHPHALELALARFDADPGISALFGSYDDTPTAPGVVSQYRNLLHHYVHQQGQFHQGI
                     RPVHTFWTGCGIIRRDVFLAFGGFDPRLYPRPAIEDIELGYRLTRAGHRIVLARDVLA
                     THLKRWTLAEMVRTDIFRRGVPWAILIKRTATVETDLNVKSEQKACVVLTVLGLIATF
                     ACAATPWAGLVALAAMSGIMLLNRGFFAFLVRRKGSAFACASFPVHLIYYCCCALSVI
                     IAEVHWYTKSHEKGVKGSTPSGRKDRAEAPIARPAASRPASRTRAPSK"
     gene            1999..3195
                     /locus_tag="VHS97_13420"
     CDS             1999..3195
                     /locus_tag="VHS97_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3449253.1"
                     /translation="MRIGIDGSCLSNRRGFGRFARRLLHALAEQCSAHDFVVFVDQPS
                     AENVLVPLDFLRVIVPVREAPTKAASAQGRRRWRDMFAMGLAVARSRIDLMYFPATYS
                     FFPVWNVPKLVVTMHDTLPFSNPELVFPGGKGRLAWRLKETAAARWADRIVTVSEASK
                     KALQAWSGWPDSRIRVITEGPDPIFCPRPDDPDAGATLGRYGISPDARYLLYVGGLSP
                     HKNLLRLVDAFAQAAEHDTLLVLAGDVNDVFHTHVPQIRAAIAQHGLQDRVILTGFVP
                     DDDLVFLYSRAVALVLPSLMEGFGLPAVEAMACGTPVVSSRAGSLPEVVGDAGVYFEP
                     TDVSSIAAAIESLLNDQGRRESLARRAVQQAAFFTWDASARALLACFDELAPKPNDRS
                     TVARSA"
     gene            3377..3967
                     /locus_tag="VHS97_13425"
     CDS             3377..3967
                     /locus_tag="VHS97_13425"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3449254.1"
                     /translation="MSTIAPMQPATMPSSVYRINVHEYERIIAARAIEDERVELIDGY
                     LVKKMGKNPPHSWSTKVLLKALERLLIPGWTWRLEQPVRIPEYNEPEPDIAIVRGTDD
                     DYKHRTPEPADVAFLVEVSESTLDRDQGEKLSAYALGGVPVYWIVNLVDGQVEVYSGP
                     SAAGYQFRHIFGPGHEVPVVIDGKEMGRIAVIDVLP"
     gene            4154..>4914
                     /locus_tag="VHS97_13430"
     CDS             4154..>4914
                     /locus_tag="VHS97_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3449255.1"
                     /translation="MCAQRIRMGRLFAAVFAVIGLSLWHPVQAQEKSATPTLGTIERI
                     DPRFDQLVPRNARVERLAEGFDWSEGPVWDKKGNYLLFSDVPLNTVFKWQEGKGVSIF
                     LKPSGYTGTVPRGGEPGSNGLLMDSQGRLILCQHGDRRVARLEQDGKFTTLADRYMGK
                     RFNSPNDGVFKSNGDLYFTDPPYGLLGLNKDRTKELEFNGVYRISASDGTVTLITKEM
                     TFPNGIALSPDEKTLYVANSDALKAIWMAFPLNVDG"
BASE COUNT          921 a         1509 c         1481 g         1003 t
ORIGIN      
        1 gccaggctct cgggcgctcg aggcgcaaat acgtgagata cttgatcgcc gacgtgcgcg
       61 cgagtctcaa tcagccgacc caggacgatc tcgaggaaga gttgatcgat ttaaacttga
      121 tccattactg tcggccgttc atgaagcacg gggatggtcg gggatgaatg gctgactggc
      181 atgataacca tttcacgcaa gaggggttca ccatcctgct ttgagcgggt cgaggacctg
      241 ccttcgaaat cctgccggga ccaagttcat ggccgcttgg aagtgacagc gttccgctcg
      301 gcgccctcgg cccacaaccg gagaagcttg agcaattcgc ccagctttac aaggccctca
      361 gcgtccgctc ttgctgtttg tttcaagtga gttccgcccg cgagagccgg gaagcgagag
      421 cgcacacact catcgctcgc taaattcagc ttttgggcac taatcacgtt tccgcgcttt
      481 gcacgggcgc acaacgcccc gaaaagccga ttcggccggc acactcgggt ggacctgacc
      541 ctacttcatg ccgctggtga tgctcgaatt ggcttacttg gctgacgcat tctgggcaag
      601 gttgacagat cttgcggttc gggctacatt tccgccgcac gtcgcgactg tgttgtgtcg
      661 gctcggttgg acatacatct cgaaatagcc cctggcgatc aatcgccccc gatctggcgc
      721 cgtgcggtga aggtacgtgt tcgggcgatc ccacggatgg agacgcactc cggcgcgtgc
      781 cgcgttctga cgggcgaggg tgaatcatgg acgatgtccc cgccggtccg gcgagcccgt
      841 ggccgcggag gccagcgctc tcggtcgttg ttcccgtgcg aaacgggggt cgcgacttcg
      901 aacgctgcct ccagcggctt cgcgactcgc aggtgaccga attcgagctg atcgttgtcg
      961 acgacggctc gactgacgac acggcgacgc tggccaaaac ggcgggggcg acggttgttc
     1021 atcaccccta tccgcaaggc cccgccgccg cacgcaacct cggtgcccgg gtcgccacgg
     1081 ccccttttat cttcttcctc gacgccgacg tcgccataca cccacacgct ctcgagttgg
     1141 cgttagcccg gttcgatgct gacccgggga tctctgccct cttcggctcg tacgacgaca
     1201 cacccactgc ccccggcgtc gtgagccagt accgcaacct gcttcatcac tacgtccacc
     1261 agcagggaca gtttcaccag ggaatccgac ccgtccacac cttctggacg ggctgcggga
     1321 tcattcgtcg cgacgtgttt ctggcattcg gcggttttga cccccggctc taccctcggc
     1381 cggccatcga ggacatcgaa ctaggttatc gcctgacccg tgccgggcat cggatcgtgc
     1441 tcgcacgcga cgttctggca actcacttga agcgctggac gttggccgaa atggtacgaa
     1501 ccgacatttt tcgccgcggc gtgccttggg cgatcctcat caagcggacg gcaaccgtcg
     1561 agaccgactt gaacgtcaag tccgaacaga aggcgtgcgt cgtattgacc gtcctcggct
     1621 tgatcgccac cttcgcgtgc gcagccacgc catgggcagg tctcgtcgcc cttgcagcca
     1681 tgagcgggat catgttgctc aatcgcggat tctttgcctt cctggtacgc cgcaaaggat
     1741 cggctttcgc ttgcgcatcc tttcccgtgc acttgattta ctactgctgc tgtgcgctct
     1801 ccgtcataat cgctgaggtt cactggtaca caaagagtca cgaaaagggc gtcaagggtt
     1861 caaccccgag cggacgcaag gatcgcgcgg aggccccgat cgccaggccc gcggcgtcgc
     1921 gcccggcgtc gcgaacgcga gccccgtcaa aataactttc gcggctcgaa acctacggac
     1981 tcccatcaga ggcccgtcat gagaattgga atcgatggca gctgcctctc gaatcgtcgc
     2041 ggctttggcc ggtttgcgcg gcggctcttg cacgcgctcg ccgaacagtg ctcggcccat
     2101 gatttcgtgg tcttcgtcga tcagccgtcg gcggaaaacg tcttggttcc tctggatttt
     2161 cttcgggtga tcgtcccggt gcgcgaagcg cccaccaaag cggcgtcggc ccaggggcgc
     2221 aggcgctggc gtgatatgtt cgccatgggg ctggccgtcg cccgatcgcg catcgacctc
     2281 atgtactttc cggccacgta cagctttttc ccggtctgga atgtgccgaa gctggtggtg
     2341 acgatgcacg acacactccc cttttccaac cctgagctgg tgtttccagg cgggaaagga
     2401 aggctggcgt ggcgtctcaa ggaaacggcc gcggcgcgat gggccgaccg gatcgtgacc
     2461 gtctctgaag cctccaagaa agccctccaa gcctggtcag gttggcctga tagccggatt
     2521 cgagtgatca ccgaaggccc cgatcccatc ttttgtcccc ggcccgacga cccggatgcc
     2581 ggagccactc tagggcgcta tggcatttcg cccgatgcgc gttatctcct ctatgtagga
     2641 ggactcagcc cccacaaaaa cctgctccgc cttgttgatg cctttgccca agccgccgag
     2701 cacgacactt tgctggtgct tgccggcgac gtcaacgatg ttttccatac ccatgtcccc
     2761 cagattagag cggccatcgc gcaacatggt ctgcaagatc gcgtgattct gacggggttt
     2821 gttcccgacg acgacctcgt gtttctctat agccgcgcgg tagccctggt cttgccgtcg
     2881 ctgatggagg gtttcggatt gccagccgtc gaagcgatgg cctgcggcac tccggtcgtc
     2941 tcgagtcgcg ccggctcgtt gcccgaagtc gtcggtgacg cgggtgtcta cttcgagccg
     3001 actgatgtca gttccatcgc ggcagcaatc gagtctcttt tgaacgatca gggacgtcgc
     3061 gaatcacttg ctcggcgggc cgtccagcag gccgcgtttt tcacctggga cgcttcggcc
     3121 cgtgcgctgc tcgcatgttt cgacgagctc gcgccaaagc cgaatgatcg atcgacggtc
     3181 gcccgcagcg cctgagcctg ctcgattacc aggccgcgga gttcgcaaaa ttcttggcgt
     3241 cagacgaagc cggtggacgc ttgcggtctt tggtacaatc gaaagctgag aaacggggca
     3301 gtcgtgacgg cgcgaacgag agggatcgcg gttgcgcacg agccggtttt ctcgaaacga
     3361 tagcggaggt ctcggtatgt ccacgatcgc gccgatgcaa ccggctacaa tgccgtcatc
     3421 ggtctatcgc ataaacgtcc acgagtacga acgaatcatc gcggcgcgtg caattgaaga
     3481 cgaacgggtc gaacttatcg acggatactt ggtaaagaag atgggtaaaa acccgccgca
     3541 tagttggtcg accaaggtgc tcctgaaagc tctcgagcga ctattaatcc cgggctggac
     3601 atggcggctc gaacagccgg tacggatccc cgagtacaac gagccggagc cagacatcgc
     3661 gatcgtacgg gggaccgacg acgactacaa gcaccggacc ccggagccgg ccgacgtggc
     3721 gtttttggtc gaagtttccg agtccacgtt ggatcgcgat caaggggaga agctttcggc
     3781 ctatgccttg ggaggtgtcc ccgtgtactg gatcgtcaat ctggttgacg gccaggtcga
     3841 agtctacagc gggccgagcg cggcggggta tcaattccgg cacattttcg gtccagggca
     3901 cgaggtcccg gtcgtcatcg acggcaagga gatgggccgc atcgccgtca ttgatgtgtt
     3961 gccttgaagg cctcgttcgg ccaaactgcg tccgccgtga cgagcgcgtg accttgtatc
     4021 cgattggctg taattgccgc ggcggtatca cagcgttgtc ctgcggcctc gagcggtata
     4081 acctcagtca aaatgccgat ccggcaacga ttcaacttcg tcgatcgact ccacgctaga
     4141 ggatattgcc accatgtgtg cacaacgaat ccgaatggga cgcctattcg ccgccgtgtt
     4201 cgccgtcatt gggctgagtc tttggcaccc ggtccaagct caagagaagt ccgcaactcc
     4261 cactctgggc acgatcgagc gcatcgatcc gcggttcgac cagctcgtgc cgcgaaatgc
     4321 gcgcgtcgag cggctggcgg aggggttcga ttggtccgag gggccggtgt gggacaagaa
     4381 gggcaactat ctcttgttct cggatgttcc gctcaatacc gtcttcaagt ggcaagaagg
     4441 caagggagtg agcatcttct tgaagccgag cggctatacc gggacagtcc cgcgcggggg
     4501 agagccgggg tcgaacggat tgctgatgga cagccaagga aggctaatcc tctgccagca
     4561 cggcgaccgt cgggtcgcgc gtctcgaaca ggacggtaag ttcacgaccc tggccgatcg
     4621 ctacatgggt aagcggttta acagccccaa cgacggggta ttcaaatcga acggcgatct
     4681 ctacttcacc gaccctcctt atgggctgct cggtctgaac aaagaccgca ccaaagagct
     4741 ggaattcaat ggcgtctacc ggatttccgc cagcgacggc acggttactt tgatcacgaa
     4801 ggagatgact ttccccaacg ggatcgccct ttcgcccgac gaaaagacgc tctatgtggc
     4861 gaattccgat gcgctcaagg caatctggat ggcgttcccg ctcaacgtag acgg
//
LOCUS       DATAQL010000771         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4658834, whole genome shotgun sequence.
ACCESSION   DATAQL010000771 DATAQL010000000
VERSION     DATAQL010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4658834"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..574
                     /locus_tag="VHS97_13435"
     CDS             158..574
                     /locus_tag="VHS97_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX3449256.1"
                     /translation="MASRANGTFEVKMNAQPPVENVGDPSVGRLSIDKQFHGDLDATS
                     KGEMLAAGTDVKGSAGYVAIERVTGALHGRTGSFTLLHRGVMTRGEPHLSVVVVPDSG
                     TGELVGLAGTMAINVGDGQHSYDFEYTIGESGKPER"
     gene            697..1890
                     /gene="moeB"
                     /locus_tag="VHS97_13440"
     CDS             697..1890
                     /gene="moeB"
                     /locus_tag="VHS97_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX3449257.1"
                     /translation="MIFPDLLGDRISSVALTSSEIQRYARHLIMPEVAMEGQRRLKTA
                     KVLCIGTGGLGSPLSLYLAAAGVGTLGLADFDVVDLSNLQRQIIHFTPDIGRPKIASA
                     LEKLKAINPELTINCHEHAIDSSNALDLFADYDVIVDGTDNFPTRYLVNDACVLLGKP
                     NVYGSIFRFDGQATVFFPPHGPCYRCLYPEPPPPDLVPNCAEGGVLGILPGLIGVVQA
                     TETVKLILGAGKPMIGRLMLYDALEMTFRELKVRKNPRCPICGTDPTIRELIDYQEFC
                     GVRGALPPQSTTGGTELAEITPIELKAALATSHPPLILDVRNPEEVAICRIAGSTVIP
                     LPELPNRLGELDRSRSMVVHCKSGARSAKAISLLQGAEFSGLKNLKGGILAWINEVDP
                     SLPKY"
     gene            complement(1906..2556)
                     /locus_tag="VHS97_13445"
     CDS             complement(1906..2556)
                     /locus_tag="VHS97_13445"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX3449258.1"
                     /translation="MSAENSRTTTIGETEPFQNNNLIVAPGSAEPEVIGADKSPQTSV
                     AAAAGTEGEETLWEGLYSSKNFLVRAIFGGLLTAAWVLVAVYAWGFGHTNFTLVAYML
                     GAGVLAYWLFLGFKYLRVRRNHHYRLTTRRLFLTTGILRRRVDQVELVRVKDLYVRQS
                     LLGSWLDVGTIVLVSSEATLPKALLLGIEEPRRVMDLIWYHTRSERDERTTEVNRI"
     gene            2862..>2990
                     /locus_tag="VHS97_13450"
     CDS             2862..>2990
                     /locus_tag="VHS97_13450"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HEX3449259.1"
                     /translation="MSLVGQKQLVDKLRELARNLWWTWQPNVLALFRELDPALWRKV"
BASE COUNT          613 a          882 c          901 g          594 t
ORIGIN      
        1 acacgagatg aggtttacgt agggcgggcc gtccggcccg ccatagcggc ctgcgagatc
       61 aagtttactg acgcaagata tcgctctttg agagccggat ccgcgctcat aacggggatt
      121 tgtaccaacg tggtcttcgg aaaaaggagg gcccatcatg gcgagccggg caaacggaac
      181 gtttgaagtg aagatgaatg ctcagccgcc ggtcgagaac gtcggagatc cgagcgtagg
      241 caggctgtcg atcgacaagc agttccacgg cgacctggac gcgaccagca agggagagat
      301 gctggccgcc gggaccgacg tcaaaggatc ggccggctac gtcgcgatcg agcgggtgac
      361 aggagcgctg catggtcgca ccggcagctt cacactactt caccgaggcg tcatgacgcg
      421 cggagagccc cacctgagtg tcgtcgtggt tcccgactcg ggaaccggtg agttggtcgg
      481 cctggccggg acgatggcga tcaatgttgg tgacggacag cattcgtacg acttcgagta
      541 cacgatcggt gagagcggaa aacctgaaag atgacagctc tattccggat cgaggggatc
      601 tgccgatcgg tctggtaagc ggccactcga ttgcgttcaa tagtcaatga acaaccgcga
      661 tgaacttgtt tccttctttt ttggtcgcag tcatccatga tcttccccga tttgctgggc
      721 gatcgcatca gttcggtcgc cttgacctca tccgaaatcc agcgctacgc tcggcatctg
      781 atcatgcccg aagtggccat ggagggccaa aggcggctca agacggccaa ggtgctctgc
      841 atcgggaccg gcgggctggg atcgccgctc tcattgtatc ttgccgcggc gggggtcggc
      901 acgctggggc tggctgactt tgacgtggtc gacctttcca atctccagcg ccagatcatt
      961 cacttcacac ctgacatcgg ccggcccaaa attgcaagcg ccctcgaaaa actcaaggcc
     1021 atcaatccgg agctcacaat caactgccac gagcatgcga tcgacagctc caatgccctc
     1081 gacctgttcg cggactacga cgtgatcgtc gacggcacgg ataactttcc cacgcgctac
     1141 ctggtcaacg atgcgtgtgt acttctgggc aagcccaatg tttacggctc gatcttccgc
     1201 ttcgacggcc aggcgaccgt cttcttcccg ccgcacggcc cgtgctatcg ctgcctctat
     1261 cccgaaccgc cccctcccga cctggtaccc aattgcgccg aggggggcgt cctggggatc
     1321 ctgccgggct tgatcggcgt cgtccaggcg accgagacgg tcaagctcat tctcggagcc
     1381 ggtaagccca tgatcggccg gctcatgctg tacgacgcgc tcgaaatgac cttccgggag
     1441 ttgaaggtcc gcaagaaccc cagatgcccc atctgcggga cagatccgac aatccgcgag
     1501 ctgatcgact atcaggagtt ctgcggcgtg cggggggccc taccgccgca gtcaacgacc
     1561 ggcgggacgg aactggcgga aattacgccg atcgagctca aggctgcgct tgcaaccagc
     1621 catcctccct tgatcctcga cgtgcgtaac cccgaagagg tcgccatctg ccgcattgcc
     1681 ggctcgacgg tcatcccctt acccgagctg cccaatcgcc tgggcgagct cgacagatcg
     1741 aggtcgatgg ttgttcattg caagagcggc gcccgcagtg ccaaggcgat ttccctgctt
     1801 cagggggcgg aattctcagg gctcaagaat ttgaagggcg gaatcttggc ctggatcaac
     1861 gaagtcgatc ccagtctgcc gaaatactga tctccggcaa atcgatcaga tccgattgac
     1921 ctcggtggtc cgctcgtcgc gctccgaacg ggtgtgatac cagatcaagt ccatcacgcg
     1981 tcggggctcc tcgataccga gaagcagtgc cttgggcaaa gtcgcttcgg acgagaccag
     2041 gacgatggta ccaacgtcga gccaagagcc gagcaggctc tgccgcacgt acaggtcttt
     2101 gactcggacc agctcgacct ggtcaactcg gcgtcgaagg ataccggtgg tcaggaagag
     2161 tcggcgagtg gtgagccggt aatgatggtt gcggcgaaca cgcaagtatt tgaaccccaa
     2221 gaaaagccaa tacgcgagca cccccgcgcc gagcatgtac gccacgagag taaaattcgt
     2281 atggccgaaa ccccacgcgt aaactgcgac caaaacccac gccgcggtca ataatccccc
     2341 gaaaatcgca cggaccagga agtttttcga ggaatacaga ccttcccaaa gtgtttcctc
     2401 gccttcggtc ccggctgcgg ccgctactga ggtttggggg gatttgtccg ctccaatcac
     2461 ttcgggctct gccgaccccg gggccacgat caaattgttg ttctgaaacg gttcggtttc
     2521 cccgatcgtt gtggtccttg agttttccgc tgacacggct atgacctcct ttgacttcag
     2581 gtcccggaag cgacgtcaac gctgattctt aacggctctt gtcgtcgatt gagaatcggt
     2641 cgcaaatctg gggccaatgc aaactcccgg gagaatcgcg ggttagataa tcaatccgtg
     2701 accaaatacg cggtcttggc gtgccgctgg tcatcggaga tactatgctg cctttggttg
     2761 agcgcggtgc cggtcgttgc caggaagtcc gttggtaggt tcccggcgcg cggtcagggt
     2821 agcccgaggc atagcctcga gtatggacga gcgagggggg tgtgagcttg gttggtcaaa
     2881 agcaattggt agataagctt cgagagttgg cccggaatct ctggtggacg tggcagccga
     2941 atgtcctcgc tctctttcgc gagctcgacc cggcactgtg gcggaaggtc
//
LOCUS       DATAQL010000772         7822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2521152, whole genome shotgun sequence.
ACCESSION   DATAQL010000772 DATAQL010000000
VERSION     DATAQL010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521152"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1147
                     /locus_tag="VHS97_13455"
     CDS             80..1147
                     /locus_tag="VHS97_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3449260.1"
                     /translation="MADSWGPNGTSDRLNGPEAAELSRQIAGLAQAGLPLASGLVALG
                     EELPRGRLRRSMNELSATLESGATLDQAVQIQNKRIPPHLRGLVIAGLRSGRLGDILS
                     RFSEYVGVGTELKRRLWLNLAYPILTVGMALALFFFVCVILVGQFDAIYRDFGIPLPR
                     LTLALIAVSHVVNQAWAPLAIVVGALCCGWLAAHIFLPVSVRRGLAGRLALVGPVWRS
                     TSIAEFCHLLALLLESRLPLPEAIRLTGEGVQDASIDSSCRLMARDVESGRSLSDAMA
                     ARPLFPQGLPRLLRWGENGKSLPEVLQMAGAMYESRARSHSTLVGAILNVLCVLLVMA
                     MVMVVPALFVPLITLISRLSG"
     gene            1173..2498
                     /locus_tag="VHS97_13460"
     CDS             1173..2498
                     /locus_tag="VHS97_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3449261.1"
                     /translation="MATGPTEPFARQETYWGEEGAAPAFAAAKGEKSPRDMDQDDYHS
                     PQRSTPWRLSHMMYLVAGVAVLLWVVVLVYDSILLGFFILAGIVFAFAMVMGGGVIQA
                     RRRSTRQDSLLWVLAIASEHAMPLAPAVAAFADQYWGPAYARIMGLAARLQGGAMLPE
                     ALEQSRGVVSRDAVLLAWVGQAAGKLPRALRLAATTRSTQLPIWTSIAARLSYILGVL
                     LVMQTITSFILYFIIPKFEAIFNDFSLSLPTITIWVIDASHFLVKFGGISFVFLILEL
                     GLLIFLPFSFQSWGNHNIPLLDRLLGRMHTALVFRSLGLMVEGDKPILLALSTLANHY
                     PTGWVRRRLRHVWSDVRDGADWIESLRRYGLIRSSDAEVLSSAAAVGNLAWALHELAE
                     TAERRLATRVLAAVQMLFPLMVVFLGAMVFIIGVAYFTPLVHLISRLAG"
     gene            2498..2926
                     /locus_tag="VHS97_13465"
     CDS             2498..2926
                     /locus_tag="VHS97_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449262.1"
                     /translation="MTARSNKTARHGSLLAEVTMAAVLLMIAMTLTVKVMGYAGVERR
                     ATERRQRALVEVANLMERITAYEFDLVTAGLASRMTLSDETRRALPDPELVVDVAEKV
                     PGAGRRAKRIGIKLRWRGRSGEWERPVRLTSWIERREEAS"
     gene            2923..3492
                     /locus_tag="VHS97_13470"
     CDS             2923..3492
                     /locus_tag="VHS97_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449263.1"
                     /translation="MKAPSAGRKSGLARRGITIIEVLIVVTTVTLMLGLCAVSIQLLM
                     KLNGDGVSRYGAAVALERLAQQMRDDAHSSESGQLGVDQKAEGKPESLWLTLGPDRSV
                     AYRSLDGAVVRTESAASKVVRRESYSLPAGAAARFLLREEGPSRLVVLVVTRGPGKSQ
                     TEPPRALELVAQIGKDRLRTSEPTGVTSR"
     gene            3489..3917
                     /locus_tag="VHS97_13475"
     CDS             3489..3917
                     /locus_tag="VHS97_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449264.1"
                     /translation="MIVKKTVRSSRGLTVVVVLVCLIIITLVSGSVLKIGLAQRELAL
                     AQERRLQAEWLAESGAQHALARLSADSTYTGETWSVSAQDLGQSEQASESAATVAIAV
                     ERVAADPGRRRVLVRADYPRDPPRRARHTREFMISLKPSQ"
     gene            3929..4879
                     /locus_tag="VHS97_13480"
     CDS             3929..4879
                     /locus_tag="VHS97_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449265.1"
                     /translation="MIRAVYGHRSQAGWAHRAPSDRAGFTLIELLVVIAIIAVLIALL
                     LPAVQAAREAARRAQCLNNVMQLGIALQSYESSHELLPPGVVNTTGPILDQPKGYHYG
                     WLVQILPFFEQRNVYNHLNFKIGIYETENFTTRTTVIRSFICPSDSSANPGASGVALT
                     NYVGSHNDVEAPISATNNGVLFLNSAVRFEDITDGSSQTIFMGEKLNNAPDEGWASGT
                     RASLRNSGSGINPTATPPFAGPGSGDDDDGDSQVAGARAASATDTLSYVGGYSARHPG
                     GANFAFGDGSVRFLKNSINRRVFRLLANRADGEIISADKY"
     gene            4898..5791
                     /locus_tag="VHS97_13485"
     CDS             4898..5791
                     /locus_tag="VHS97_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449266.1"
                     /translation="MSRPRAFTLLELLIVVGIIAVLIALLLPAIQAAREQARRAQCVN
                     NLLQLGIAMGNYASTHSVLPPGVANDKGPIVSQPVGYHHGWTVQILPFIGQDNIYRRF
                     DLGESVYAPSNETARSVMIATFLCPSSSRVGSSNGPISYAGCHHDVEAPIAADNHGVL
                     YLNSHVRFDDITDGLAQTILLGELRSDEPAILTWASGTRATLRNTGAHLNSQNPLTAL
                     YAGIAQLSEADKQAAAARMFDEGAIPVDFVGGFSSWHPHGGNFAFCDGSVRFVKQTVA
                     QRVYQFLGNRADGELISDDSF"
     gene            5953..7605
                     /locus_tag="VHS97_13490"
     CDS             5953..7605
                     /locus_tag="VHS97_13490"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HEX3449267.1"
                     /translation="MLTWMRIVLSGLIVLSVGSATVLAGGPDSNASAEKFRADPATVQ
                     RFGSGYRYPQAGWIVLHIEGEPYERGYQHGRLMAAEIAGFIGEVARYRSAKAPADAWE
                     DLRLIADALFLRRFDAEFLEEMKGIADGAAAAGARWEGRPVDLLDLVTINADVETNFL
                     ENALEATATGLEGRRFREPLQQGPARTPESHCSAFAATGPATADGQVVFGHITMWNLF
                     HAYHYNVWIDVKPARGHRVLMQTYPGGIMSGLDYYMNDAGIVVCETTIAQTKFDAAGI
                     PLVDRIRRALQYGDSIDQAVEILRQGNNGLYTNEWLLADTKTNEIAMFELGTHKSRLW
                     RSSRDEWFGGTPGFYWGCNNAKDMQVRLETVPALAGRPANVVFHPSDRDRTWLSLFDR
                     KNKAIDVDFGFLAFTTPPLAAAHSLDAKFTTTAMARELKTWAKFGPPLGRTWEPTDAE
                     RRRFNEIRPLIGNDWAVLSVDPPAMPATPGARAVDLARIAPVNDQKPENVHAPSWHGT
                     ILPATDADLWLAAAFADYERIVARESAIKAGASGGKLGIRDR"
BASE COUNT         1387 a         2355 c         2517 g         1563 t
ORIGIN      
        1 cagaatttgc gtgctccgga cgagcgagtt taacgtagca acggtgggcg aagcccacct
       61 tccgaactaa agggttggaa tggccgattc gtggggaccg aacgggacaa gtgacaggct
      121 gaacggcccc gaggccgcgg agttgtcacg tcagattgcc gggctggcgc aggcgggcct
      181 gccgctggct agcggcttgg tcgcacttgg cgaagagttg cctcgcggcc ggctgcggcg
      241 atcgatgaac gagctgtcgg cgacgctcga atcgggtgcg acgctggatc aggctgtcca
      301 gatccaaaac aagcgcattc ccccccatct gcgtggtctg gtaattgccg ggctgagatc
      361 gggtcgactg ggggatatcc tgagccggtt ttccgaatac gtcggcgtcg ggacggagct
      421 caagcgcagg ctgtggctga acctggctta tcccattttg actgtcggca tggcgctggc
      481 acttttcttt tttgtctgcg tcatccttgt cggtcagttc gacgcgatct accgtgactt
      541 cggcattcct ttacccaggt tgacgcttgc tctgatcgcg gtttcccacg tggtgaatca
      601 ggcctgggcc ccgctggcga tcgtcgtagg cgcgctctgc tgcggctggc tggcggccca
      661 catctttttg ccggtgtccg tgcggcgcgg cctggcgggg cggctggcgc tggtgggacc
      721 ggtctggcgc tcgacgtcga tcgcggaatt ctgccatttg ctcgctctgc ttttggaaag
      781 ccggttgcct ctgcccgagg ccatccggtt gacaggcgag ggcgtccagg acgcgagcat
      841 cgactcttcg tgccggttga tggccaggga cgtcgagtcg ggccgttcgc tgtcggacgc
      901 catggcggcg cgccccctct ttccccaggg gctccctagg ctcttgcggt ggggcgagaa
      961 cggtaagagc ttgcccgaag tgctccaaat ggccggcgcg atgtacgagt cgcgtgcgcg
     1021 ctcgcattcc acgctggtcg gtgccattct caatgtactc tgcgtcttgc ttgtcatggc
     1081 catggtgatg gttgtcccgg ctctttttgt gccgctcatc accttgatca gtcgtctgtc
     1141 gggatgagat cgacccttcc agggagaaga ccgtggccac gggacccacc gagccgttcg
     1201 cgagacagga aacctactgg ggtgaggaag gggctgcgcc tgcattcgcc gcggcaaagg
     1261 gagagaaatc gccccgcgat atggaccaag acgattatca ctcgccacag cggtcgacgc
     1321 cgtggcggct ctcccacatg atgtatctgg tcgccggggt cgctgtactc ttgtgggtgg
     1381 tggtcctggt ctacgactcg atcttgctgg gctttttcat cctggcgggc atagtcttcg
     1441 cgttcgcgat ggtgatgggc gggggggtta tccaggcacg ccggcgatcg acccggcagg
     1501 attctctgtt gtgggtgctg gcgatcgcgt ccgagcatgc catgccgctg gcccccgccg
     1561 tcgcggcgtt cgccgaccag tactggggcc cggcttatgc ccgaatcatg ggcctggccg
     1621 cccgcttgca ggggggagcg atgctccccg aggcgctcga gcagtcgcgc ggggtcgtgt
     1681 cgcgcgacgc ggtattactc gcctgggtcg gacaggcggc cggcaagctg ccgcgtgccc
     1741 tgcgcttggc cgcgacgacc cgttcgaccc agttgccaat ctggacgtcg attgccgcca
     1801 ggctttccta catactgggc gtgcttttgg tgatgcagac catcacatct ttcatattat
     1861 attttattat acccaagttt gaggcgatct tcaatgactt cagtttgtct cttcccacga
     1921 tcaccatctg ggtcatcgat gccagtcact tcttggtcaa gtttggcggc atcagcttcg
     1981 tgttcctcat actcgagctc ggcttattga tcttcctgcc gttttcgttc cagtcctggg
     2041 gcaatcacaa catacccctc ttggacaggc tgctgggccg gatgcatacc gcgcttgtgt
     2101 ttcgttcact gggcctcatg gtcgaagggg acaaaccgat cttgctcgcc ttgtcgaccc
     2161 tggcgaacca ctatccgacc gggtgggtcc ggcgcaggct gagacatgtc tggtccgacg
     2221 tcagagacgg ggccgactgg atcgagtcgc tcaggcggta cgggctgatc cggtcgtcgg
     2281 atgccgaggt gctttcgtcg gctgcggcgg tggggaacct ggcctgggcc ttgcacgagt
     2341 tggcggaaac ggccgagcgc cggctggcga cccgagttct ggcggcggtc caaatgcttt
     2401 tcccactcat ggttgtattc ctcggtgcaa tggtctttat cattggcgtc gcttatttca
     2461 cgccgctggt gcatctcatc tcgagattgg cggggtaatg acggcacgat cgaacaaaac
     2521 ggcgcggcac ggctcgctct tagccgaggt gacgatggcc gcagtattgc tcatgatcgc
     2581 catgacgctg acggtcaagg tgatgggcta cgccggggtc gagcggcgcg cgacggagcg
     2641 gcgacaacga gcgttggtcg aggtggccaa cttgatggag cggatcacgg cgtacgagtt
     2701 cgacctggtc accgccggcc tcgctagccg gatgaccctc tcggacgaaa cgcggcgggc
     2761 tctccccgat cccgagctgg tcgtcgacgt cgccgagaaa gtacccggtg ccggccgaag
     2821 agccaagcgg atcggtatca agctgcgctg gcgtggccgc agcggcgagt gggagaggcc
     2881 ggtgcgcctg acttcctgga tcgaacgccg ggaggaagcc tcatgaaagc gccgtctgcc
     2941 gggcggaaat ccgggctagc tcgccgcggc atcacgatca tcgaggtcct gatcgtggta
     3001 accacggtca cgctgatgct gggcctctgt gcggtttcga ttcagctctt gatgaaactg
     3061 aacggcgacg gagtctcacg ctatggtgcg gccgtcgcgc tcgagcggct ggcgcaacag
     3121 atgcgcgacg atgcgcactc gagcgagagc ggccagttgg gcgttgacca gaaagccgaa
     3181 ggcaagcccg agagcctatg gctcactctc gggcccgatc gctcggttgc gtatcgatct
     3241 ctcgacggtg cggtggtccg taccgagtca gcggcgagca aggtagtccg acgtgagtcg
     3301 tattcactgc cggccggcgc cgccgcgcgg tttcttctgc gtgaggaagg gccgagccgg
     3361 ttggtcgtcc tggtagtgac gcgcgggcct ggcaagagcc agaccgagcc gccgcgtgcg
     3421 ttggaattgg tggcgcagat cggcaaggac cggctgcgca cgtcggagcc aacgggagtc
     3481 acatcgcgat gatcgtcaaa aaaaccgtca gatcgtctcg aggattgacc gtggtcgtgg
     3541 tgctcgtttg cctgatcatc atcacgctgg tgagcggtag cgtgctcaaa atcggtctgg
     3601 cccagcgcga gctggcgctt gctcaagagc gccggcttca ggccgagtgg cttgccgagt
     3661 cgggcgcgca gcacgcactt gctcgcctct cggccgacag cacgtataca ggcgaaactt
     3721 ggtcagtcag tgcccaggac ctcggacaga gtgagcaagc ctcggaatcg gccgcgaccg
     3781 tggcgatcgc cgtcgagcgc gttgcggccg acccaggtcg ccgccgggtg ctcgtaaggg
     3841 ccgactatcc gcgcgacccc ccgcggcgcg cgcgccatac cagagaattt atgatctccc
     3901 ttaagccaag ccaatgagga accgtcatat gatccgcgct gtttatggac accgatcgca
     3961 agccggatgg gctcaccgcg caccgtccga tcgtgccggc tttaccttga tcgaactcct
     4021 ggtggtcatt gcgatcattg ccgtgctgat tgctctctta ttgccggcgg tacaggccgc
     4081 gcgcgaggca gcccggcgcg cccagtgcct taacaacgtc atgcagctcg ggatcgcgct
     4141 gcaaagctat gaatcgtcgc atgagctgtt gccgccgggc gtcgtcaaca ccaccggccc
     4201 gattctcgat cagcccaagg gataccacta tggctggctg gttcagatct tgcccttttt
     4261 cgagcagaga aacgtttata atcatcttaa cttcaaaata ggcatttatg agaccgaaaa
     4321 tttcaccacc cggacgaccg tgatccgcag cttcatttgc ccgtccgact caagcgccaa
     4381 tcctggtgcc agcggggttg ccctgacaaa ctacgtcggt tcgcacaatg atgtcgaggc
     4441 gccgatttcc gccacaaaca acggcgtact ttttctcaac agtgccgtac ggttcgaaga
     4501 catcaccgat ggcagctcgc agacgatctt catgggtgaa aagctcaaca acgcgccgga
     4561 cgagggctgg gcatcgggta cgcgagcgag cctgcggaac tccggatcgg gaattaaccc
     4621 gacggccacg ccaccgttcg ctgggcccgg ttccggcgat gatgatgatg gcgatagcca
     4681 ggtcgccggt gctagggccg cgtcagctac cgacaccttg agctatgtcg gcggttactc
     4741 tgcccggcat cctggcggtg caaatttcgc gttcggcgac gggtcggtcc ggtttttaaa
     4801 gaattcgatc aatcgacgag tcttccgctt gctggccaac cgcgccgacg gcgaaatcat
     4861 cagcgcggac aaatactgac gcccagggag acgatccatg agccgcccac gggccttcac
     4921 gttgctcgag ctcttgattg tggtcgggat catcgcagtg ttgatcgcgc tcttactccc
     4981 ggcgatccag gcggcgcgcg agcaggccag gcgggcccag tgcgtaaaca acctgttaca
     5041 actgggtatc gctatgggga attatgcctc gacccactcg gtcttgccgc cgggcgtggc
     5101 gaacgacaag gggccgatcg tgagccagcc ggtcggatac caccacggat ggaccgtgca
     5161 gattctaccg tttatcggcc aggacaatat ttatcgccgg ttcgatctgg gagaaagcgt
     5221 ttacgcgccg agcaacgaga cggcgcgatc ggtcatgatt gccacttttc tatgcccttc
     5281 ctcttcccgg gtggggtcaa gcaacggacc gatcagctat gcgggctgcc atcacgacgt
     5341 cgaggcgccg atcgcggcgg acaaccacgg cgtgctctac ctgaatagcc acgtccggtt
     5401 cgatgacatc accgatgggc tggcacaaac gatactcttg ggagagctaa gatctgatga
     5461 acccgcaatt ctgacctggg cgtccggtac gcgcgcgacg ttgcggaaca ccggagccca
     5521 tttgaatagt cagaaccctt tgacggcgct ctatgccggc atcgcgcaac tcagcgaagc
     5581 ggacaaacag gcggccgccg ccaggatgtt cgacgaaggg gcaattccgg tcgacttcgt
     5641 gggcggattt tcgagctggc atccccacgg aggaaatttc gctttctgcg acggctcagt
     5701 ccgcttcgtc aagcaaacgg tcgcgcagcg tgtctatcag tttctgggca atcgtgccga
     5761 tggcgaactg atcagcgacg attcgttctg aggccggtgg acttggtcca gtgctgattt
     5821 ccgagcactc gccaggctag cctctcgcgt gcgcggttcg cgcctattaa ggtggggggg
     5881 gatacgctcc ggatcacaac cggtgtgcgc tgtatcaccg acaagcggac cctagccctg
     5941 ccgaggatga tcatgttgac gtggatgcgt atcgtcttga gtggtctgat agtgttgtct
     6001 gtaggatcgg ccacagttct ggcgggcggt ccggattcca atgcaagcgc cgaaaagttc
     6061 cgagccgatc ccgcgacagt acagcggttt ggctcgggtt atcgctaccc ccaagccggc
     6121 tggatcgtct tgcacatcga gggagagccg tacgagcggg gataccagca cgggcggttg
     6181 atggcggctg agatcgccgg gtttatcggc gaggtggccc gctaccgaag cgccaaggcc
     6241 cctgccgatg cgtgggaaga cttgcggttg atcgcggacg ccctgttcct gcggcgtttc
     6301 gatgccgagt ttctcgagga gatgaaagga atcgccgatg gcgcggcggc ggccggagcc
     6361 cggtgggagg ggcggccggt cgatctgctc gacctggtga cgatcaacgc cgacgtcgag
     6421 accaactttc tcgaaaacgc gctcgaagcc actgcgacag gcctcgaagg ccggcggttt
     6481 cgcgagcccc tccagcaagg gcctgctcga acgccggaat cgcattgcag cgccttcgcg
     6541 gcgactgggc cggccacggc cgatgggcag gtcgtctttg gccacatcac catgtggaat
     6601 ctttttcacg cgtaccatta caacgtctgg atcgacgtca aacccgcgcg gggacatcgc
     6661 gtcttgatgc agacgtatcc cggcggaatc atgagcggtc tcgattatta catgaacgat
     6721 gcgggcatcg tcgtttgcga gacgacgatc gcccagacca agtttgacgc agccggcatc
     6781 ccgctggtcg accgcatccg ccgcgcgctc cagtatggcg actcgatcga ccaggcagtc
     6841 gagatcttgc gccagggaaa caacggactt tacaccaacg agtggctgct ggcggacacc
     6901 aagaccaacg agatcgcgat gttcgagctg ggcacgcaca agagtcggct ctggaggagt
     6961 agcagagacg agtggttcgg tggcacgccc gggttttatt gggggtgcaa caacgcaaag
     7021 gacatgcagg tgcggctcga aactgtgccg gccttggctg gccggccggc caacgtcgtg
     7081 ttccacccca gcgatcgcga ccggacgtgg ctatcgcttt ttgaccgcaa gaacaaggcg
     7141 atcgacgtgg attttggttt cctggctttt accactccgc cgcttgcggc cgctcactct
     7201 ctcgacgcca agtttaccac cacggccatg gcacgcgagc tcaagacgtg ggcgaaattc
     7261 gggcccccct tgggccgaac ctgggaaccc actgacgccg agcgccgtcg attcaacgag
     7321 atccgccccc tgataggcaa tgattgggcg gtgctcagcg tcgacccgcc ggccatgccg
     7381 gcgacacccg gcgcgcgagc ggtcgacctc gcccgcatcg cgcccgtgaa cgatcaaaag
     7441 ccggaaaatg tccacgcgcc gagctggcac ggcacgatct tgcccgctac cgacgccgat
     7501 ctctggctgg ccgcggcatt tgccgattac gagcggatcg tcgcacgcga aagcgcgatc
     7561 aaggccggag cttcgggcgg caaactgggt atccgtgacc gctagcggct ggccctggcc
     7621 tactttgcgc caacctcgcg ttacctgaca gccgtcgccc gccgtggcgg ccaagacatc
     7681 ccccttgcag aaattcacgc cgacctgcgc tccgacgaat ggtacgacat cgcgtcggga
     7741 aaaggggtgc tcgtacttgc cgagctgcgt gacatcatgg gagcgaagca attcgacaag
     7801 ttcatggatg attttggccg gg
//
LOCUS       DATAQL010000773         4167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_841591, whole genome shotgun sequence.
ACCESSION   DATAQL010000773 DATAQL010000000
VERSION     DATAQL010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841591"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VHS97_13495"
     CDS             <1..702
                     /locus_tag="VHS97_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3449268.1"
                     /translation="ARQGRPFVRRFVEERALSLWLVVDVSASMRFGPERTTKADRAAQ
                     AAALLATAAIQNGDRAGLVLVSDRIELELAPAGGVRHLSQLVRALVATPTSSPKTKLS
                     VGLARVRRTARRAMLVVISDFITEEPVGSWRRAARRHDTIALRIVDSREEKIPVAGLL
                     AVEDAEHGRSFMVDSGSAKVRRRYERAALDRSQSFLRWCANSGIQAFTMATDVDPIIP
                     LIELFSKRATRRGGR"
     gene            699..1292
                     /locus_tag="VHS97_13500"
     CDS             699..1292
                     /locus_tag="VHS97_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449269.1"
                     /translation="MNKPGGPRLVPPRPNLGPESWSDARPSPIAVIVAAALGGLLIAW
                     LLWRWRRKRRSHIRGDKKTQSAHDATPQGRLVAFSRSIRETLADQFGTAWRAKTTQEL
                     ATEALLVEVLGREQLDELIRFLELVDHLKFAPERSNNHHESLEGVLTNWEPRIVAIGK
                     KIQTKPERKLKPATADSRPQNSKPRVSRAEIAGVPLK"
     gene            complement(1307..2278)
                     /locus_tag="VHS97_13505"
     CDS             complement(1307..2278)
                     /locus_tag="VHS97_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449270.1"
                     /translation="MTQEIASKPLPTPNPEGHHAAGSIRRTAGGSDETLQRNDDFAVG
                     EPNYGSDADDLDLILSLPVTDPDPALRSKASSDVMRPTPGSSSADDHFADSRLYWALI
                     VLMSYSSAVTLALTWVLWSGWGSRPAEPPVASADQNQAPVESPTKIVESRPVIELAPL
                     PVGNVTTLAKTVRIGDLEVTPLKVIVTRLRLARSIPPHNRRREAGASVVLRLRLTNLS
                     KDQSFAPLEPAFVRDQSSPLDRSSIATSGGKNIGLFPLAIDSEWSIAGQDFPVLKPGK
                     SAETIIASELGAEDRLTSDMTWRVRLRIGPYKTDMIGVRFDKNEVTR"
     gene            complement(2275..3090)
                     /locus_tag="VHS97_13510"
     CDS             complement(2275..3090)
                     /locus_tag="VHS97_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX3449271.1"
                     /translation="MAPGILTLTTDFGSVGPYVAALKGVLLELAPGTQFVDVCHTISP
                     QNILEGGFVLAGIVDVFPKGTVHLAVVDPGVGTARRLIAVAVADQWFVLPDNGLITGV
                     ARGRIPDAIWEITNPAMRRTVVSATFHGRDILAPASAHLLRGGNPAQLGPARDKFIML
                     RNFEPNADGLGFVGEVIFRDAFGNLITNVNVEHLADVPRDAWEIEIANERIDGILRTY
                     GDRPAGTLIALVGSSGWVEVAVVNGDAGRQLTAGAGTTVWCRRKSLPTRWSPS"
     gene            complement(3181..3531)
                     /gene="queF"
                     /locus_tag="VHS97_13515"
     CDS             complement(3181..3531)
                     /gene="queF"
                     /locus_tag="VHS97_13515"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX3449272.1"
                     /translation="MPSFVETFPNQFPQREYEIEVTCPEFTAVCPKTGQPDFGTIIID
                     YIPAATCLELKSLKLYLFGFRDRGIFYEHVVNTILDDLTASCQPRKMKVVGQFNPRGG
                     ITSRITAVYEAKRS"
     gene            complement(3844..>4167)
                     /locus_tag="VHS97_13520"
     CDS             complement(3844..>4167)
                     /locus_tag="VHS97_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449273.1"
                     /translation="GRASVAGNLQTTGFIAEGHAHGTMTLTLGRGTITLQLTGVPVQG
                     GFAGLPSTFTYTVTSGTGAYRNVTDHGIATLTTAQGNSMNQSEHGAFTLNLRSSNTKI
                     AMPVL"
BASE COUNT          799 a         1264 c         1296 g          808 t
ORIGIN      
        1 gcgcggcaag gccggccgtt cgtgcggcgg ttcgtcgagg agcgggccct ttcgctctgg
       61 ttggttgtcg acgtgtcggc gagcatgcgg ttcggaccag aacggaccac caaggccgat
      121 cgggccgctc aggcggccgc gcttttggct acggccgcca ttcagaacgg cgatcgcgct
      181 ggactcgtgt tggtgagcga tcgaatcgag ctcgagctcg ccccggccgg tggggtgcgt
      241 catctctcgc agttggtgcg cgcactcgtg gcgacaccga caagctctcc caaaaccaag
      301 ctgagcgtcg ggctggcgcg cgtgcggcgg accgcgcggc gagccatgct ggtcgtcatc
      361 agcgacttca tcaccgaaga acccgtcgga tcatggcggc gcgcggcccg ccggcacgac
      421 acaatcgcct tgcgaatcgt cgattcccga gaagagaaga tacctgtcgc gggcttgctc
      481 gcggtcgagg acgccgagca cggtcgatca ttcatggtcg attcgggctc ggccaaggtc
      541 aggcggcgat acgaacgtgc cgctctcgac cgatcgcagt cgttcctccg ctggtgcgcc
      601 aactccggaa ttcaggcgtt cacgatggcg accgacgtcg atccgatcat acctctgatc
      661 gagctcttct cgaaaagagc gacgcgacga ggtggtcggt gaacaaaccc ggaggcccta
      721 ggctcgtgcc accgcgcccc aatcttgggc ccgaatcgtg gtcggacgca cggcccagcc
      781 caatcgccgt catcgtggca gccgctttgg gcggactact catcgcttgg ctcctgtgga
      841 gatggcgtcg caagaggagg tctcacatcc gtggtgataa gaaaacgcag agcgcgcatg
      901 acgccacgcc ccaaggacga ctggtggcgt tttcccgttc gatccgcgag actctggccg
      961 accagttcgg cacagcctgg cgcgccaaga caacccagga gctggccacg gaggccctgc
     1021 tcgtcgaggt gctgggacgc gaacaactcg acgaactgat tcggttcctc gagctcgtcg
     1081 atcacctcaa gttcgcgccg gaacgttcga acaaccacca tgaatcattg gagggcgtcc
     1141 tgacgaactg ggaacctcgg atcgttgcta tcggcaagaa gatccagacc aaacccgaac
     1201 gaaaactcaa gcccgcaacg gctgattccc ggccccagaa ttccaaaccg agggtcagcc
     1261 gggccgaaat cgccggtgtg ccgttgaaat gacgaattct tgatgcctag cgggtcactt
     1321 cgttcttgtc gaaccgcacg ccaatcatgt cggttttgta cggaccgatc cgcaaccgca
     1381 cgcgccaggt catgtcggac gtcaatcggt cctcggcgcc gagctcactg gcgatgatcg
     1441 tttcagcgct cttacctggt ttcaagaccg ggaagtcttg tccggcgatc gaccactcgc
     1501 tgtcaatcgc caggggaaaa aggccgatgt ttttcccgcc tgatgttgcg attgacgacc
     1561 gatccaaagg cgagctctga tctcttacaa atgcgggttc gagaggagcg aacgactggt
     1621 ccttggagag attggtgagc ctcagtcgaa gtacgaccga cgcgccggcc tcgcgccgcc
     1681 gattgtgcgg cgggatcgat cgagccagcc tcaatcgagt aacgatgact ttgaggggcg
     1741 tgacctcgag gtcgccgatc cgtacggttt ttgccaatgt tgtcacatta cccacgggaa
     1801 gcggcgccaa ctcgatgacc ggcctggatt cgacgatttt cgtcggagat tcaacggggg
     1861 cctggttctg gtcggcactg gcgactggcg gttcggcggg gcgagatccc caccccgacc
     1921 agagaaccca ggtcagagcc agtgtgaccg cgctcgagta gctcatgagc acgatcagtg
     1981 cccagtaaag gcgcgagtca gcaaaatgat cgtccgcgga cgacgaacca ggcgtgggac
     2041 gcatcacgtc ggatgaggcc ttcgatcgca acgccggatc cgggtcggtc accggcagcg
     2101 acaagatcaa gtcgagatcg tccgcgtcag acccgtaatt tggctcgccg actgcgaagt
     2161 cgtcatttct ctggagcgtc tcatcgcttc cgccagcggt tcgtcgaatg gatccggcgg
     2221 catggtgacc ttccggattc ggagttggga gcggcttgct ggcgatctct tgagtcatga
     2281 tggcgaccat ctcgtgggta atgattttcg gcgacaccac actgtggtac ctgcgcccgc
     2341 ggtgagctga cggccggcat ctccgttgac caccgcgact tcgacccagc ctgagctgcc
     2401 gaccagggcg atcagcgttc cggcgggccg gtcgccgtaa gttcgcaaga ttccgtcaat
     2461 tcgctcgttg gcgatctcga tttcccaggc atcgcgggga acgtcagcga gatgttcgac
     2521 attgacattg gtaatcaggt tgccgaaggc gtcgcggaag atcacctctc caacgaagcc
     2581 tagcccatcg gcgttgggct caaagtttct gagcatgata aacttgtcgc gagcgggccc
     2641 gagctgggcc ggattaccac cacgaagcag atgagccgag gccggcgcga gaatgtcgcg
     2701 gccgtgaaac gtggccgaga caacggtgcg cctcatggca ggattggtga tctcccagat
     2761 tgcgtccggg attctcccgc gagccacgcc tgtgatcagg ccgttgtctg gcagcacgaa
     2821 ccattgatcc gcaacggcca cggcgatcag cctgcgcgcg gtccccactc ccgggtcaac
     2881 gaccgccaga tgaacggttc ccttgggaaa cacatcgaca atgccggcca gaacaaaccc
     2941 cccctccagg atgttttggg gcgagatcgt atgacagacg tccacgaatt gggtgccggg
     3001 ggccagctcg agcaacaccc ccttcagtgc ggccacgtag gggccgaccg agccgaaatc
     3061 ggtcgtcagc gtgagaatcc ccggtgccat tttgcctccc aacctgtcaa gaggccgtgc
     3121 ggcccagcca cgcggccagg ccgggtcgcg cgatcgacct gttccagatt cgcgacctcg
     3181 ttaactgcgt ttcgcctcat agacggcggt gatccgcgag gtgatgccgc cccgcgggtt
     3241 aaactggccc acaactttca ttttccgtgg ctggcagctc gctgtcaggt cgtccaggat
     3301 cgtgttgacc acatgctcgt agaatatgcc ccggtctcga aaaccgaaga ggtacaattt
     3361 caggctcttg agctcgaggc aggtcgcggc ggggatgtaa tcgatgatga tggttccgaa
     3421 atccggttgg cccgtcttgg ggcataccgc ggtgaattcc ggacacgtga cctcgatctc
     3481 gtattcccgt tgcgggaatt gattcgggaa ggtctcaaca aaggaaggca tgtcggttcc
     3541 tcggaactgg ccggggcagg catccgcttg gggaagggat cgcggttgta gtcgttgctg
     3601 gtgccgacgg gcgccagtac accgttccgg gcgattcgaa tcgcgactct ccttcccgcc
     3661 ccggcctcag cttttgattg tactcaaaac ccatctctac tcgaaagttg actcgagctg
     3721 attattatag ccggactaat tcccagcaaa gcggccttgg ttggatgtgc ttggagtcaa
     3781 agttggtttg ggtggcgccg tcgcaaccag ggccctgtga accaagcccc accggtccac
     3841 cgcctagaga acgggcatcg cgatcttggt gttcgacgag cgaaggttga gtgtgaacgc
     3901 accgtgctcc gattgattca tgctgttccc ttgcgcggtc gtcagggtag caataccatg
     3961 gtcggtgacg ttacggtaag caccggtccc cgaagtcaca gtgtaggtga aggtactcgg
     4021 cagtcccgcg aatccgcctt gcacgggtac tccggtaagc tgaagtgtga tcgtgcccct
     4081 gccaagcgtc agagtcatcg ttccgtgggc gtgcccttcg gctataaagc cggtcgtttg
     4141 caagttcccc gcgacggacg cgcgccc
//
LOCUS       DATAQL010000774         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3286783, whole genome shotgun sequence.
ACCESSION   DATAQL010000774 DATAQL010000000
VERSION     DATAQL010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3286783"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VHS97_13525"
     CDS             <1..592
                     /locus_tag="VHS97_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449274.1"
                     /translation="PAPGAAGGAGAPDPAAYARASNMPQIPGGPPGAPGAAPGAMQPG
                     GPGAAIGDAGGDNGPADFRTPMGAARSFLSALKSKDLERLADTVALRAPTEASSKSNQ
                     KLFESILERSMSEDDLSDLANKLNGFQMVDANQPKSSGRFSIILGKPGKNGEFYRRTI
                     TLRHEKAGWKVLDISGQGKIEKPIVMPRGRGGMRRR"
     gene            849..1175
                     /locus_tag="VHS97_13530"
     CDS             849..1175
                     /locus_tag="VHS97_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449275.1"
                     /translation="MVSLSLRISAILELVVILFQIVGVAALCVSRLLPSTRARWANRG
                     RVGFVVAMIGLGFAGAVCGGQDSEFGLFAGGTMTILLIGMIVGNGHTDATVATRSCVA
                     EPKLAG"
     gene            1551..>1879
                     /locus_tag="VHS97_13535"
     CDS             1551..>1879
                     /locus_tag="VHS97_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3449276.1"
                     /translation="MLVGLKRISTALGLVAGLAAGAHAQGVAPSANQATANAVAGALQ
                     SSPSLSGYRIEVESREGLVTLSGTVKTRAQKAEALALAERVGGVRGVVDHLKLSRADR
                     LRRVQYQP"
BASE COUNT          324 a          595 c          610 g          350 t
ORIGIN      
        1 ccccgcgccg ggggctgccg gcggtgcagg cgcgcccgac cccgctgcct acgccagggc
       61 ctccaacatg ccacaaatcc ccggcggccc gccgggtgct cccggtgccg cgcctggagc
      121 catgcagccc ggcggccctg gcgccgccat cggggatgcg ggaggtgaca acggtccggc
      181 tgactttcga acacccatgg gcgcagcccg ctcgtttttg agtgctctca agtccaagga
      241 cctcgaacgc ctggcagaca cggtcgccct gcgcgcaccg accgaggcct cctccaagtc
      301 gaaccagaag ctcttcgagt cgattctcga acgcagcatg tccgaggacg acctgagcga
      361 cctcgccaac aagctcaatg ggttccagat ggtcgacgcg aaccagccga agagctcggg
      421 gaggttctcg atcatcctcg gcaagccggg gaagaacggc gaattctacc gccgtacgat
      481 cacgctccga cacgaaaagg cgggctggaa ggtgctcgac atctcgggtc agggcaagat
      541 cgagaagccg atcgtgatgc cgcgcggacg aggaggcatg cggcgccgtt gaccaaacgg
      601 caagaggcgc gaattgttgt aagaatgacg gtcattgcgt gtaaagattt ccgaccctcg
      661 gccagaaatc tggccgaggg tctttttttt cctcaagttg cgttcgcgaa ttgccgggat
      721 gcgcggccgg aacccgtttt tttgagtttg gcctgtaacc tgcattatcc acacaggccg
      781 ttcaagttgc tgcaacctgc cgcaatgatt gtcgacatct ccagacagtg cccaaggaag
      841 gagcctgtat ggtaagtttg agcctgcgca tttcggcgat tctcgaactc gtcgtcatcc
      901 tgttccagat cgtcggcgtg gcggcgctct gcgtgagccg gctactcccc tcgactcggg
      961 cgcggtgggc gaatcgggga cgcgtcggat ttgtcgtcgc gatgatcggc ctcggcttcg
     1021 ccggcgcggt ctgtggcggc caggactcgg aattcggcct tttcgccggc ggcacgatga
     1081 cgatcctatt gatcggcatg atcgtgggca acggacacac tgacgcgacc gtcgcaaccc
     1141 ggtcttgcgt ggccgagcca aagctcgccg gctgagcgaa tttcgatttt ttaaatccct
     1201 atcccgtatt tggttagcga cgcggggctg cccggccccg cgattttttt tgcgcgcgat
     1261 ttgaccggcc tggaatcgcg cgccatagtt gcggcgacga ttcggagacg atggaccggt
     1321 acgctcgcgc tccctcccga cctcacacct ctttggtcct ctctcgaacg tgtacggttc
     1381 gcacctggtt cgcggtggaa gcgccgcggg gcagggggga gttgcccggc gattcggacc
     1441 gtctccgaag gcgcactcac cacggaccca aacgtgaatc cgaacgacgt gtcccgctcg
     1501 ccgttcgttc cgaggccgtg ttggcccctt ggcgggagaa ggataaagcc atgttggttg
     1561 gattgaaacg gatttccacc gcgctgggac tggtcgcagg tctggccgcg ggagcgcacg
     1621 ctcagggggt tgctccgagc gccaaccagg cgacagccaa cgcggtcgcc ggggcacttc
     1681 agtcgagccc atccctgtcg ggatatcgaa tcgaggtgga gagccgtgag ggcctcgtca
     1741 cgctcagcgg caccgtgaag acccgggccc agaaggccga ggccctggcg cttgccgagc
     1801 gcgtcggcgg ggtgcgcggc gttgttgacc atctcaagct ctcccgcgcc gaccggttgc
     1861 ggcgggtcca gtatcagcc
//
LOCUS       DATAQL010000775         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_691453, whole genome shotgun sequence.
ACCESSION   DATAQL010000775 DATAQL010000000
VERSION     DATAQL010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691453"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VHS97_13540"
     CDS             complement(<1..745)
                     /locus_tag="VHS97_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449277.1"
                     /translation="MRAIVARPASWVFLILLCSYAFFWHSRDWNTASRLMLTYALVDR
                     GTVAITGLDRQTNDKAWFQGEYYSDKLPGFSFLGAVPYAVARWVFRLPSHPLNVAAFP
                     FWAADYWVTLGTSGLATAAIGSILVLWARELGCRPGRATIVGLAYGLATPAYVYATLA
                     YGHQVSAFALFMSFFLLWRRRQARDALGLVLAGFLAAYAAVIELQVGPVSAILGLYLL
                     AQCLRGDCRPDALAVFAIGAAIPTLILLAY"
     gene            complement(749..2164)
                     /locus_tag="VHS97_13545"
     CDS             complement(749..2164)
                     /locus_tag="VHS97_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX3449278.1"
                     /translation="MTCCVYLVQSLNRARLLFNMGITPSDQRIHAADSPQTFRSWFWK
                     AAAIGCAALCVFAFSLSAKRFEDEDAYISQSYYTDLFFSGRFHDHAWLEFPAYDLQPL
                     PKYLIGFSLRLAHLPVPAPRNAADWYRSYKPFGTYPTLIVARIPFITLGAFGVVAIFA
                     CGTLLKDWRVGAVAALLLTLNPLYRLHAHRAMSDTPCEAFMLAGLALCLWAWRRTWSG
                     KIGPSVLLVPCLAGICFGLSVLCKFNGILGLVIIAAWCCLAMIAAGLDVSRKLAIAGQ
                     SIVVFIVALAVVVGLNPFMTARPRGLMAGGQPDLALVNVWDRFDFQVKHRLELSDNQK
                     RQFPDDALYTLADKAKVVAVQGFGRFGPFGPSDSDSTVRFDLRQDWGAILWVPAVLLG
                     LVVSVLMGIYQYQTGQAPTAVALSIWAALGWVVVTLYLPMAWNRYLLPIQSGNVLLAA
                     LAAGAIWDKLAGRNSIVGTRT"
     gene            2483..>3083
                     /locus_tag="VHS97_13550"
     CDS             2483..>3083
                     /locus_tag="VHS97_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449279.1"
                     /translation="MFRLGCLQPGGRARGKPRRRGRWAALGLITCLAVVQSGCQSGPF
                     SNCGNGSGLFSPCGFFGRVSSRVFNRSNGGCCGSSGVVTEAPVEYPASPSTVVTPGAI
                     PTYPSSPGSSSTPSSGFPVPGDTPSDLSPTEPVPKARIQTPPGNGASNSTSGKQSYQT
                     RRPDSGSRIARQRNDNLTKTLVSTPEPTPRSARAPSRSSS"
BASE COUNT          712 a          943 c          913 g          515 t
ORIGIN      
        1 tgtatgccag caggatcagg gtcggaattg cggcgccgat tgcaaaaacc gccagagcgt
       61 cgggacgaca atccccgcgc agacattgtg ctagaagata gaggccaagg atggcagaca
      121 ccgggcccac ctgaagctcg atcaccgcgg cgtacgcggc cagaaagccc gcaagtacca
      181 gacccaatgc atcgcgcgcc tgccggcgtc gccagagcag gaagaacgac ataaatagag
      241 cgaacgccga cacctgatgg ccgtatgcca gagtggcata aacgtaggcc ggcgtcgcca
      301 ggccgtaggc gagcccgaca atcgtggctc gcccgggtcg acatcccagt tctcgtgccc
      361 acaagacgag aatcgacccg atggccgccg tcgcgagccc agaagtgccg agggtaaccc
      421 agtagtcggc ggcccagaaa gggaacgcag caacattgag cgggtggctc ggcagccgaa
      481 agacccacct ggccaccgca tacggcactg cccctaagaa tgaaaagccc ggcagtttgt
      541 ccgaataata ctcgccctga aaccatgctt tatcgttggt ttgtcgatcc agcccagtga
      601 tggcgacagt gccgcgatcg accagcgcgt aggtaagcat cagccggctc gccgtgttcc
      661 agtcacgcga gtgccagaaa aacgcgtagc tacagagcag aatcaggaag acccaggatg
      721 caggccgggc gacaatggca cgcaccggtc aggtcctagt gccgacaatc gagttgcggc
      781 cggcgagttt atcccagatc gctccggccg cgagcgccgc cagcagcaca ttgccactct
      841 ggatcggcag caaatagcga ttccaggcca tcggcagata gagcgtcacc acgacccagc
      901 ccagggcagc ccagatcgag agcgcgaccg cggttggcgc ctgaccggtc tgatattgat
      961 agatccccat cagaaccgac accaccaatc ccagcaggac agcgggcacc cacaggatcg
     1021 ccccccagtc ctggcggagg tcgaagcgca ccgtcgaatc ggaatcgctg ggcccgaacg
     1081 ggccgaaccg accgaaaccc tggacggcta cgaccttggc tttgtcggca agggtgtaaa
     1141 gggcgtcgtc gggaaattgc cgcttctgat tgtccgataa ctcgagtcga tgcttcacct
     1201 gaaaatcaaa gcgatcccat acgttcacca aggcaaggtc gggttggcca ccggccatca
     1261 gcccgcgcgg tcgggccgtc atgaatgggt tcaaaccgac gacgacggcc agcgccacga
     1321 tgaataccac gatcgactgg ccagcgatcg ccaacttgcg actgacgtcg agtccggctg
     1381 cgatcatcgc cagacagcac catgcggcaa taatgacgag tcccagtatt ccgttaaact
     1441 tacacaggac cgataggccg aagcagattc cggccaggca cggcaccaat aagacggatg
     1501 ggccaatctt gccggaccac gttcgccgcc atgcccagag acaaagagcc aggcccgcca
     1561 gcatgaacgc ctcgcacggt gtatcggaca tcgctctgtg cgcatgcaaa cgataaagcg
     1621 gattgagagt caacaagagg gcggcgacgg cacccacacg ccagtctttg agcaaggttc
     1681 cgcatgcaaa aatggcgacg acgccgaatg cccctaaagt gataaacggt atgcgcgcga
     1741 caatcagagt cggataagtc ccaaagggct tgtagctgcg gtaccaatca gcggcattgc
     1801 ggggagccgg caccggcagg tgggccaatc ggagcgagaa cccgatcaga tattttggca
     1861 agggttgcaa atcgtaagcg gggaattcga gccaggcgtg atcatggaaa cggcccgaga
     1921 agaaaagatc ggtgtaatag ctctgtgaga tgtatgcatc ttcgtcctcg aatcgcttcg
     1981 cggacaagct gaaggcaaag acgcacagag cggcgcagcc gatcgctgcc gccttccaaa
     2041 accaggatcg aaaggtttgc ggggagtctg ccgcgtggat gcgttggtcg gacggcgtaa
     2101 tccccatgtt gaataggagc cttgcgcgat ttagcgactg cacgagatag acacaacaag
     2161 tcattctaga aatcgatcgg tttctttggc aaccgaagga ttgtcggcta agtttcatcg
     2221 atgaccctta atgaagggtt gtcaatagag tttccatgct gttgcgccaa ttggaacgaa
     2281 cgatctacgc gaccactggg ccgtatggcg actgaataat caggccatgc ggcaaagacg
     2341 gcatgtttgt atcgcccggc accgttgtcg tcgctcctgg aaactttcac cgatcctttg
     2401 agtcggcacc ccgaacctcc cgatgtgcat cgtcttccgg aatgatgcac gcggcggtgt
     2461 cagagaacag gagaatctaa acatgtttcg tctgggttgc ttacaaccgg gcggtagggc
     2521 gcgcgggaag ccgcgccgcc gcggccgctg ggccgccctc gggctcatca cctgcctggc
     2581 cgtcgtacag agcggctgtc aatccggtcc gttcagtaat tgtggtaacg gcagcggtct
     2641 ctttagcccc tgtggcttct ttggccgcgt ttcgtcccga gtctttaacc ggagcaacgg
     2701 cggttgctgc ggcagctcgg gagttgtcac agaagccccg gtggaatacc cggcgtcgcc
     2761 gtcgaccgtc gtcacccccg gtgctattcc cacgtatcca tcaagcccgg gatcgagctc
     2821 gacaccgtcg tcgggctttc ccgttccggg tgacacaccg tcggatctgt ctcccaccga
     2881 gccggtcccc aaggccagaa tccagacacc gccgggaaat ggtgcatcca actcaacgtc
     2941 tggcaagcag agctaccaaa cgcgccgtcc ggactccggc tcgcggatcg cgcggcaacg
     3001 aaacgacaac ctgaccaaaa cgctggtctc gacgcctgag ccgaccccgc ggtcggcccg
     3061 ggcgccttca cggtcttcct cca
//
LOCUS       DATAQL010000776         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4964942, whole genome shotgun sequence.
ACCESSION   DATAQL010000776 DATAQL010000000
VERSION     DATAQL010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4964942"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1625
                     /locus_tag="VHS97_13555"
     CDS             216..1625
                     /locus_tag="VHS97_13555"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX3449280.1"
                     /translation="MAGIVKSLGCVSVWVILIGLGPVGAAESSSGWKAGVARVDTTPS
                     KPVRMAGYASRTAPSQGVAHPLAAKALALEDARDHKVVFVTCDIIAFRRAFTNRVTER
                     VKAKYGLPREDVVLFASHNHAGPSPIESKSSNSLGANSPPGEGLENNVAYTIDLENKI
                     VDLIGEALAKMQPANLSYGIGRAHFALNRREPVANGIKLGKNPAGPTDESVPILQVQG
                     TNGKPLAIVFGYACHNTTLRPDMMKIAADFAGFAQDRIEADHAGAVALFVTGCAGDDD
                     PHPFGNLEMAKNHGEELGEAVKFVLDHPTWMTNLTGPLHTAFTETTIHFGGPTDRASY
                     EKRLKDPNRGRQGHAKRMIELIDQGRPVVAEYPHYSVHAFALGDLLTLVALSGEVVVD
                     YAIRLQKELGGENQTLWVAAYANDVIGYIPSVRVLKEGGYEGGESFYGSTWPTPLADD
                     IESIVVNAAHEVVKKVRGK"
BASE COUNT          363 a          524 c          516 g          311 t
ORIGIN      
        1 cgtcgatctc ggcatgcgtg agcatagccg gcccctatac aaacacgaag cctctctcat
       61 tgattataat ttgccagatc tctctcagat ctcacgaatc acgagaagag aagcagccgg
      121 tttcttgatg ccgacccgtg agggaatgcc ggcgagacgg tgctaaaaag tttcgatgat
      181 tggacttgag cccgaaaaac aaggagcgac ggaagatggc gggcatcgtc aagagcctcg
      241 gttgcgtgag cgtatgggtc atcctgatcg gcctcgggcc agttggcgcg gccgagtcgt
      301 cgagcggttg gaaggctggc gtggcgcgcg tggatacgac gcccagcaaa ccggttcgga
      361 tggcgggata cgcatcgcgg acggctccct ctcagggcgt ggcgcacccg ctcgcggcca
      421 aggcactcgc gctagaggat gcacgcgatc ataaggtcgt cttcgttact tgcgacatca
      481 tcgccttccg tcgggccttt accaaccgcg tgaccgagcg ggtcaaggcc aagtatggat
      541 tgccgcgcga ggatgtcgtc ttgtttgcgt cgcacaatca cgccggccct tcgccgatcg
      601 agtccaagtc cagcaacagc ctgggggcga acagtccacc gggcgaggga ctcgagaaca
      661 acgttgccta cacgatcgat ctggaaaaca agatcgtcga cctgatcggc gaggcgctcg
      721 caaaaatgca gccggccaac ctgtcctacg ggatcggccg cgcgcacttc gccttgaacc
      781 ggcgcgagcc ggttgccaac gggatcaagc tgggtaagaa tcccgccggc cccaccgacg
      841 agagcgtgcc gatccttcaa gtccagggca cgaatggcaa accgctggcc atcgtctttg
      901 gctatgcctg ccacaacacc acgctccgcc ccgacatgat gaaaatcgcc gccgatttcg
      961 ccggtttcgc ccaggaccgc atcgaggccg accacgccgg cgctgtcgcg ctttttgtca
     1021 ccggctgcgc gggcgacgac gacccccatc cattcgggaa cctcgagatg gccaaaaacc
     1081 atggcgagga actgggcgaa gccgtcaagt ttgtcctcga tcacccgacg tggatgacaa
     1141 atctgaccgg tccgttgcac actgccttca ccgagaccac cattcacttc ggcggtccga
     1201 ccgaccgcgc ctcttacgaa aaacggttaa aggatcccaa ccgtggccgc caggggcatg
     1261 cgaaacgaat gatcgagctc atcgaccagg gccggcctgt cgttgcggaa tacccccact
     1321 actcggtgca tgcgttcgcc ctgggcgatc tgttgactct ggttgccctt tccggcgaag
     1381 tggtggtcga ctacgcaatc cgccttcaga aagagctcgg cggcgaaaac caaaccctgt
     1441 gggtggcggc ctatgccaac gacgtgatcg gctatattcc ctcggtgcga gtgctcaagg
     1501 agggtggcta cgaaggcggt gaatccttct acggtagcac ctggcctaca cctttagccg
     1561 acgacatcga gtcaatcgtc gtcaatgcgg cccacgaagt ggtgaagaag gtgcgtggta
     1621 agtaggacgc gtgtcaggcg acacgtgcgg ccggcgacgg atgtccggct cgaaaaatga
     1681 cggaaccaaa tcagtacgcg tttccgatgc cgtt
//
LOCUS       DATAQL010000777         4697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3132709, whole genome shotgun sequence.
ACCESSION   DATAQL010000777 DATAQL010000000
VERSION     DATAQL010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132709"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VHS97_13560"
     CDS             complement(<1..1126)
                     /locus_tag="VHS97_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449281.1"
                     /translation="MATDSDNPNPRHHSLVRGGYAFHVARRMGFEPATIPRRLTKILL
                     LILVTWVPLVVLSLLNGHAWGHSIAEPLLADPVIYSRFLFVVPLLELAQVVVETSLGV
                     QTRQFLKSGLVPQEEQPEFLMARDTVIRLRGSVVAEMLIAVLALAISFVAQVIMTDGP
                     GHSTWKRVEGTITPSGWWYILVSLPILAFFLLRWVWIFLLWAWFLFRVSHLELELTPT
                     HPDRAGGLGFLGWGLASFSLVLMAVSAVMSGGLAREIIHRGSSLDNLKYHVMIFVALA
                     IVVVHAPLLAFAGKQARCRFEGLLDFGTLIWRHDRAFDDKWVKSLDASASSLLGSNDV
                     ASLVNLGFAYEHVEKMQLIPFDKKALAVLVLATLIPMIPLL"
     gene            complement(1172..2227)
                     /locus_tag="VHS97_13565"
     CDS             complement(1172..2227)
                     /locus_tag="VHS97_13565"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX3449282.1"
                     /translation="MNWLWQVGLVVVSAVIVFAVVRARKPVPAEPHALSLGPAPPDMV
                     WIPGGEFSMGCVDPRDLPHGGHEAMTDARPIHRVSVDGFWIDQFEVTNDQFAQFVKAT
                     GYVTIAERTPTAAEFPGAPAENLVAGSVVFAPPPQQGPLDNHDRWWSYVKGANWRHPQ
                     GPSSSIDGRGGYPVVHVAYEDAEAYARWAGKRLPTEAEWECAARGGTAELLYAWGDEL
                     CPGDKWMANIWQGQFPVSDTALDGFAGIAPVGRFPPNGYGLYDMAGNVWEWCSDWYRA
                     DSYSQQAASGIAHNPQGPATSLDPAEPGQAKRVHRGGSFLCTEQYCTRYMIGTRGKGE
                     ISTGSNHLGFRCVKVAH"
     gene            complement(2399..2899)
                     /locus_tag="VHS97_13570"
     CDS             complement(2399..2899)
                     /locus_tag="VHS97_13570"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX3449283.1"
                     /translation="MDPYDGVSGFLPGRASLMLDVVVVAMFFVLLTLGFSVYSVKYGR
                     KYARHKAIQVTLASTLLVVLLLFEIDVHFIDKWMIRADASPYFDAATKSGLVIYALAI
                     HLVFATTTFALWFMVILRAWTSFPTPPGPSEHSRFHRRWGRVAALDMVMTTLTGWLFY
                     WLAFIA"
     gene            complement(2912..>4697)
                     /locus_tag="VHS97_13575"
     CDS             complement(2912..>4697)
                     /locus_tag="VHS97_13575"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3449284.1"
                     /translation="SINACKYERVLNEQNKPIGHALRDGQGKLLRRFVAVRSDHLDQW
                     SYYQDGFEVYREDDLNGDRSLDECRWLNAGGMRIAKVEKGKIKAWKQISAEEASKVLV
                     QALVSGDPALLDTVMATPEELAAAGVSKDIVAKVAETASKRPEQVAELRKKLIGWTDQ
                     TVWNRFDGTFPHVIPADPASGLEKDLTLYENAMVIPGTTAANQNPAKIAFLQIPDMIQ
                     LGTTWKFVELPRAIDPEKPIVTAASGIRAMLFDRANNVEPRDEAVDAALKTLADYDIK
                     NAPLLQGGQKADIVRYNVGRFPLLRGIVKASKNPDDQLNYNKQVVDSLVAALRTGLYP
                     QGRKPLDALIADGGKLSSYAAYSLIDAEFAMENDAPGANILANQKKWMADLDEFLKKF
                     PNSDEVPLVLLHLANANEFSMEEKKAREQYSKLVQGYAATDAGKKAAGALRRLDLAGT
                     PLVIKGTGLQNETIDTAQFHGKPVLIVFWASWASPVKTDLPDLNKLYNKYHSGGLQVV
                     GVDLDNERTELDEFLKQNKLPWPQIFEGGGMDSRLAIDYGIISLPTMFLVDGQGKAVN
                     VYVRSSDVEKQLEKLLPTKEAGIADRRQ"
BASE COUNT          963 a         1412 c         1386 g          936 t
ORIGIN      
        1 ccaaaagggg gatcatcgga atcaaggtgg ccaggacgag cacggccagg gctttcttgt
       61 cgaacgggat cagctgcatt ttttccacgt gttcgtatgc aaacccaaga ttgactagcg
      121 acgcgacgtc attactgccc aagaggctcg acgcgcttgc gtccagcgac ttgacccact
      181 tgtcgtcgaa ggcgcggtca tggcgccata tcaaagttcc gaagtccagc agcccctcga
      241 accggcatcg cgcctgcttg cccgcgaatg ccaggagtgg ggcatgaacg acgacgatgg
      301 ccagtgccac aaagatcatc acgtgatact tcaaattgtc aagtgacgag ccccggtgaa
      361 tgatttcgcg ggccaagccg cccgacatca cggccgagac cgccatcaag accagtgaaa
      421 agctcgccag gccccagccg aggaaaccca ggcctccggc gcggtcgggg tgagtgggcg
      481 ttagctcgag ttcgagatgg gagacacgga acaggaacca cgcccagaga agaaagatcc
      541 agacccagcg caagagaaag aaggccagga tcggcaggct gaccaggatg taccaccacc
      601 cggacggcgt gatcgtccct tcgacccgtt tccaggttga atgaccgggc ccgtcagtca
      661 tgatcacctg cgctacaaat gagattgcca aggcgaggac cgcaatcaac atctctgcaa
      721 caaccgagcc gcgcaggcga atcactgtgt cgcgcgccat caagaattcc ggctgttcct
      781 cttgtggaac caggcccgat ttgaggaatt gccgcgtctg gacgcccagg ctcgtctcga
      841 caacaacctg cgcgagctcc aaaagtggca cgacaaacaa gaaccggctg tagatcactg
      901 ggtcagccag cagaggttcc gcgatcgagt gcccccaggc atgaccattt aacagcgaca
      961 aaacgacgag cggcacccaa gtcacgagga tcaacagcag gatcttcgtt agccgcctgg
     1021 gaatcgtcgc cggctcgaaa cccatgcgac gggcaacgtg gaatgcgtac ccgccccgca
     1081 cgagagagtg atgacgcggg ttcggattat cagaatctgt ggccataatt cacgtgtcca
     1141 agttgaccct cacgtcgttg tggcaactcg ttcaatgtgc caccttgacg caccgaaagc
     1201 ccagatgatt gctaccggtg gaaatctcgc ccttgccccg tgtgccgatc atgtagcggg
     1261 tgcagtattg ctcggtgcaa aggaacgatc cgccccgatg cacgcgcttg gcttggcccg
     1321 gctcggcggg gtcaaggctg gtggcgggtc cctgggggtt gtgtgcgata ccgctggcgg
     1381 cttgttggga ataggagtcc gcacgatacc agtcggaaca ccattcccag acattgccgg
     1441 ccatgtcata gaggccatag ccattgggcg ggaaccggcc gaccggggcg atgcctgcaa
     1501 aaccatcaag ggcggtatca cttactggaa attgtccctg ccaaatgttg gccatccact
     1561 tgtcacccgg gcagagctca tcgccccacg cgtacagcaa ttccgccgtg ccgccgcggg
     1621 ctgcgcattc ccactcggcc tcggtcggca gccgtttgcc ggcccagcgt gcataggcct
     1681 ccgcgtcttc atacgccaca tgcactacgg gataaccacc gcggccgtcg atactactgg
     1741 agggcccttg aggatgacgc cagttggccc ccttcacata gctccaccac cggtcatgat
     1801 tgtcgagtgg accctgttgc ggcggcggcg caaacacgac cgatccggcc acgaggtttt
     1861 ccgcaggcgc cccgggaaac tccgcagctg tgggggtgcg ctcggcgatc gtgacgtagc
     1921 ccgttgcctt gacgaactgc gcgaattggt cgttggtaac ctcgaactga tcgatccaga
     1981 acccgtcgac agaaacccga tgtatcggcc gggcgtcggt catggcttca tggccaccgt
     2041 gcggcaaatc tcgagggtcg acgcagccca tagagaactc accgccggga atccagacca
     2101 tgtccggcgg agccgggccg agagacaacg cgtgcggttc cgccggcacg ggcttgcgag
     2161 cgcgaacgac ggcgaaaacg ataaccgcac tgacgaccac caggccaact tgccatagcc
     2221 aattcattgg tcgcgttttc ggcggctccg ccttcgaccg agcggttgct ttgtgcatcg
     2281 ataaggggca ttcactttgg aagcaaaatg agaaccgtcg gacgtgcacg ccgttaccgt
     2341 aacagtgagg acagccggaa gcaaccgtcc tcctgctggt acgactgtcg gagcacattc
     2401 acgcgatgaa agcgagccag tagaagagcc acccggttaa tgtcgtcatg accatgtcga
     2461 gcgcggccac tcttccccag cgccgatgga agcggctgtg ttcactagga cctggcggcg
     2521 tggggaagct agtccacgcc cggagtatga ccatgaacca gagggcaaaa gtggttgtcg
     2581 cgaagacgag atggatcgcc agggcataga ttaccagacc actcttggtc gctgcgtcga
     2641 agtaaggaga ggcatcggct cggatcatcc atttgtcaat gaaatggacg tcgatctcaa
     2701 agagaagaag tacaacgagc agcgtcgacg ccagagtgac ctgaatcgcc ttatgccgcg
     2761 cgtatttccg accgtacttg acggaataca cgctgaaacc gagcgtcagc agcacgaaga
     2821 acatcgcgac gacgaccacg tcgagcatga gcgacgctct ccccggcaga aagccgctca
     2881 cgccgtcata tgggtccatc gaggtactct ctcattggcg acgatctgca atacccgcct
     2941 ccttggtggg caagagcttt tcgagctgct tttccacgtc gctcgagcga acatagacat
     3001 tgacggcctt gccctgtccg tcgaccagga acatcgtcgg cagcgagata atgccgtagt
     3061 cgattgccag gcggctgtcc atgccgcccc cctcgaagat ctgtggccag ggcagtttgt
     3121 tttgcttgag gaattcgtcg agctcggttc gttcattgtc aaggtcgacg ccaacgactt
     3181 gcagcccgcc actatgatac ttgttgtaga gcttgttcag gtcgggcaga tcggttttga
     3241 cgggcgacgc ccagctcgcc cagaagacga tgagtacagg cttgccgtga aactgtgcag
     3301 tatcgattgt ctcgttttgc agcccggtcc ccttgatgac gagcggcgtg cccgccaggt
     3361 cgaggcggcg gagcgcgccc gccgctttct tgccggcgtc cgtcgcggcg tagccttgaa
     3421 ccagcttgct gtactgttcg cgcgccttct tttcctccat gctgaattcg ttggcgttgg
     3481 ccagatggag taatactagc gggacctcat cggaattcgg gaacttcttg agaaattcgt
     3541 cgaggtcagc catccatttt ttctggttgg ccagaatatt ggcgcctggg gcatcattct
     3601 ccattgcaaa ctcggcgtca atcaggctgt aggccgcata tgagctcagc ttgccgccgt
     3661 cggcgatgag cgcgtccagg ggctttctgc cttgcggata gaggccggtt cgcagggccg
     3721 cgaccaggct atcgacgacc tgcttgttgt aattcagttg atcgtcgggg tttttcgatg
     3781 ccttgacaat cccgcgcaag aggggaaagc ggcccacgtt gtaccgcaca atgtcggctt
     3841 tctgtccgcc ttggagcaac ggagcgtttt tgatgtcgta atcggcaagt gttttcaatg
     3901 cggcgtccac ggcctcgtcg cgcggctcga cgttgttcgc tcggtcgaaa agcatcgcgc
     3961 ggattccgct ggccgccgtg acgatcggtt tctcgggatc gatcgcgcgg ggcagttcga
     4021 caaacttcca cgtcgtgccc agctgaatca tgtcggggat ctggagaaaa gcgatcttgg
     4081 caggattctg gttggccgcc gtggttccgg ggatgaccat cgcgttttca tagagcgtca
     4141 gatctttctc caacccgctg gctggatccg ccgggatcac gtggggaaac gtgccgtcga
     4201 atcgattcca gaccgtctga tcggtccagc cgatcagctt tttgcgcagc tcggcgactt
     4261 gctcagggcg tttggacgcc gtctcggcta ccttggcgac aatgtccttc gacacaccgg
     4321 cggccgccag ctcttccggc gtggccatga ccgtgtcgag caaggcggga tcaccgctca
     4381 ccagggcctg gaccaatacc ttcgaggcct cttcggccga gatctgcttc caggccttga
     4441 tcttgccctt ttccaccttg gcgattcgca ttccgccggc attgagccag cggcattcat
     4501 caagactccg gtcgccgttg aggtcgtctt cgcgatagac ctcgaatccg tcttgataat
     4561 agctccactg atccagatgg tccgaccgaa cggcaacgaa tcggcgcaat aacttcccct
     4621 ggccgtcccg cagcgcgtgg ccgatcggct tgttttgctc gttcaagacc ctttcgtact
     4681 tgcacgcatt gatcgag
//
LOCUS       DATAQL010000778         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2371760, whole genome shotgun sequence.
ACCESSION   DATAQL010000778 DATAQL010000000
VERSION     DATAQL010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371760"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            690..1313
                     /locus_tag="VHS97_13580"
     CDS             690..1313
                     /locus_tag="VHS97_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3449285.1"
                     /translation="MADASDAGSITNCLMSRERGDRQAAQAIWDRYFQRLVSLARPRL
                     QSAHLRAVDEEDVVLSALDSFFSRAGSGQFPRLEDRDDLWKVLFVLTVRKAISLVRHE
                     RRPSRGGGWLSVLSDLEGLDVEEVIGDVPTPELAAQMADECHRLLGRLGNATLCQVAV
                     WKTEGYTNVEIAGKLGCVTHTIERKLRSIREHWSKDRGGAGDSPGRS"
     gene            complement(1397..>1586)
                     /locus_tag="VHS97_13585"
     CDS             complement(1397..>1586)
                     /locus_tag="VHS97_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449286.1"
                     /translation="ACQRLRNAACTNPRLASALLRRKCRIEHHRQPHSSLSQTLPHYR
                     QLLRIHGFRPSFKFVRHA"
BASE COUNT          264 a          509 c          542 g          271 t
ORIGIN      
        1 cttgcagatt ctgtagttgc tggaattcta gagagtgagg cccggtcagc aaccgacggc
       61 cctccaacga ctcgagctgg gggctcgatc gcctgcggtt attcggcctc gtacgcgtcc
      121 ggcgcttcat cagactgagt gctggcgttg ccatggggaa tctcctcggg tgtgtaccgt
      181 tcgacaaatt ctcggaaggt tgcccgccgc ggccacatcg gccgcggctt gaggctccgc
      241 tgcgtcgacg accggccgga acccgaatcg ccacggccga tccgtgcgtg ggccgagacc
      301 gattcgggtc cgttgacggt cgtgagacga ttggcaaagg ccgctggcac tggcctcccg
      361 gcggcccggc gggagaggtc cgccccgtcc cagattcgac cgagtccagg ccgtgtccct
      421 cgcgtcgccc ctcccagcaa gaaaatcaac ggaaaccccc gtaacccaac acgcgccttc
      481 gggatttttc ggcgcacgat gacctgcgtg ggggcgcgac gccgagcggt cgcggaccgc
      541 tgtccccatg tcgcctcctc ccagccttcc gtcggcgagg ccagggtact gggctgtcgc
      601 ggtgtgccgt cgcgttccct tcgagctcga cggcacttat aaacgaggtc ggtcgacggt
      661 ctgttcggtt acccgtggag gccgcgacta tggccgatgc ctcggacgcg gggtcgatca
      721 cgaactgcct catgtcgcgt gagcggggcg accggcaggc ggcccaggcg atctgggacc
      781 gatacttcca acgcctcgtc agcctggcgc gcccccgcct ccagtccgcc cacctgcgag
      841 ccgtcgacga ggaggatgta gtcctcagcg ccctcgacag cttcttcagc cgcgccggga
      901 gcggccagtt tcctcggctc gaggatcgcg acgacctgtg gaaggtgctg ttcgtcctga
      961 ccgtgcgcaa ggcgatcagc cttgtccggc acgaacgacg gccatcgcga gggggcggtt
     1021 ggctcagcgt cctgtcggac ctcgaaggcc tcgacgtcga ggaggtcatc ggcgacgtcc
     1081 cgacaccgga gttggccgct cagatggccg acgagtgcca tcggctcctc ggccggctcg
     1141 gcaacgcgac attgtgtcag gtcgcggtgt ggaagacgga ggggtacacc aacgtcgaga
     1201 tcgccggcaa gctcggctgc gtcacgcaca ccatcgagcg taagctgcgg tccatccgcg
     1261 agcactggtc gaaggaccgt ggcggcgcag gcgatagtcc cgggcggagc taggacggct
     1321 gatccatcgg gcgagagccg cgccttgtcc aagatacgcg tgggccgcga aacggagtga
     1381 cggcaagcgc ggaagatcac gcgtggcgaa caaacttgaa acttggcctg aacccatgaa
     1441 tgcgaagaag ctgtcggtag tgaggcagcg tctggctcaa gctggaatgc ggctggcgat
     1501 ggtgctcaat gcggcatttg cggcggagta gtgccgaggc cagccgtgga ttagtgcacg
     1561 ccgcgtttcg cagtcgttgg cacgca
//
LOCUS       DATAQL010000779         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2371767, whole genome shotgun sequence.
ACCESSION   DATAQL010000779 DATAQL010000000
VERSION     DATAQL010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371767"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VHS97_13590"
     CDS             complement(<1..693)
                     /locus_tag="VHS97_13590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449287.1"
                     /translation="MSINTAQSPIGSGRVYPWVVVGLLWFCGFFNYADRQAVYSVFPL
                     LKSEFGLSQRQLGYLGSAFMLVYALTSPFSGYTVDLLPRRLLITLGLTFWSLICAATA
                     LSRSFLQLIFFRAAEGLGESFYFPASMSILADYHGGRTRSRALGIHQTSVYLGTAGGA
                     VLAGYLGERLGWRSPFWVLGLAWVVYAVLLGFVLIEPRRESTDRDEARRDEEADDPWN
                     QSPPSSDSLAMKL"
     gene            complement(703..1905)
                     /locus_tag="VHS97_13595"
     CDS             complement(703..1905)
                     /locus_tag="VHS97_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX3449288.1"
                     /translation="MSNQPASSMAHLVVAVTLLALVSAAQAAEPNCERMAIFPANPKH
                     NHASCVVELQDGSLLAAWYSGSGERTADDVVIEGAWLKKGKTAWSSTFLMADTPGYPD
                     CNPALFAAPDRTLWVFWPTILDHRWEGALLKYAHRDQPMGEPTAWKWAASGILHITPK
                     DFPTEVDRAIASLSDDEKSKYSRTLGELKKRAGDELYQRLGWMPRVHPLALPSGRWIL
                     PLYSDTFSGSIMAISDDRGETWTASRPLIGFGNIQPSLVRKNNGELVAFMRDNGPHHR
                     IRLSRSRDDGVSWSPVVDSPFPNPGAGIEAIQLASGRWALVYNDLPSGRYSLAVSVSD
                     DEGETWKWTRHVERTERGEGQFHYPSMIQAADGMIHVTYTWSRPGRGSRIEHARFNEA
                     WLMADDLK"
     gene            complement(2011..>2197)
                     /locus_tag="VHS97_13600"
     CDS             complement(2011..>2197)
                     /locus_tag="VHS97_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449289.1"
                     /translation="RAQGNRLEAPATGRNKPSLLINGADYNQRLLSNHPLRLPTAGST
                     VPATRREAGKGTKGRVR"
BASE COUNT          456 a          707 c          655 g          379 t
ORIGIN      
        1 cagcttcatc gccaacgagt cggaacttgg cggtgattga ttccacggat cgtcggcctc
       61 ctcgtcccgt cgtgcctcgt cgcgatcggt ggattcgcgc cgcggttcga tgaggacgaa
      121 accgagcaag accgcgtaga caacccacgc cagacccaac acccagaacg gcgatcgcca
      181 gcccagacgc tctcccaagt aacccgccag gaccgccccg ccagccgtcc ccagatacac
      241 gctcgtctga tgaataccca gcgcccgcga ccgtgtgcgg ccgccatgat aatcggccag
      301 aattgacatc gaggccggga agtaaaacga ttctcccagg ccctcggcgg cccgaaagaa
      361 gatcaactgc aagaaactgc gcgaaagcgc cgtggccgcg cagatcaaac tccagaatgt
      421 gagtcccagc gtgatcaaca gccggcgggg caagagatcg accgtgtaac ccgaaaacgg
      481 tgatgtcagg gcatacacga gcatgaacgc cgaccccaga tatccaagct gccgctgact
      541 gagcccaaac tcactcttga gcaacggaaa caccgagtag accgcctgcc ggtccgcata
      601 attgaagaag ccgcaaaacc agagcaagcc cacgaccacc catggataca cccgtcccga
      661 gccaattggc gattgcgctg tattgatgct cacgttttct ctttatttca ggtcgtctgc
      721 catcagccag gcctcgttga accgcgcgtg ctcgatcctg ctgccccggc ccgggcggct
      781 ccatgtgtat gtgacgtgga tcatgccgtc ggcggcctga atcatcgagg gataatgaaa
      841 ttggccttcg ccgcgctcgg tccgctcgac atgccgcgtc catttccagg tctccccttc
      901 gtcgtccgag accgaaacgg ccagcgagta ccggcccgag ggcaaatcgt tgtagaccaa
      961 agcccagcgg ccactcgcca gctggatcgc ctcgatgccc gcgccggggt tgggaaaggg
     1021 agaatcgacc accggcgacc aagatacgcc gtcgtcgcgc gaccgcgaaa gccggatgcg
     1081 atgatgagga ccgttgtcgc gcatgaaagc caccaactca ccgttgtttt tccgcaccag
     1141 gctgggctga atgttgccga agccgatcaa gggccgactg gccgtccagg tctcaccccg
     1201 atcgtccgaa atcgccataa tcgagccgga aaacgtgtcg gaatagagcg gcaagatcca
     1261 acggcccgaa ggaagcgcga gcggatgaac ccttggcatc cagcccagtc gctgatagag
     1321 ctcatcgccg gcgcgctttt tgagctcccc cagcgtccgg ctgtacttgc ttttctcgtc
     1381 gtcgctcaaa gaggcgattg cccggtcaac ctcggtgggg aagtctttgg gcgtgatgtg
     1441 caagatgccg gacgcggccc atttccacgc ggtcggctct cccatcggct ggtcgcggtg
     1501 ggcgtacttg aggagcgcgc cttcccaacg atggtcgagg atggtcggcc agaagaccca
     1561 gagcgtcctg tcgggcgcgg cgaagagagc cggattacaa tccggatagc cgggcgtgtc
     1621 ggccatcaaa aacgtggagc tccatgccgt cttccctttc ttgagccatg cgccttcgat
     1681 cacaacgtcg tcggccgtac gttcgccgct gcctgaatac caggccgcca ggagcgagcc
     1741 atcctgcagc tcgaccacac aggaggcatg gttgtgcttg ggattggccg gaaaaatcgc
     1801 catcctctcg cagtttggtt cagccgcctg cgcagccgag actagagcga gcaaggtcac
     1861 ggccacgacc agatgtgcca ttgatgaagc cggctggttg ctcatctcaa gacgatctcc
     1921 ttgatgacgc ggggagagga atccaaccat cgcggcagtg gcaccgagtt tacgagcacc
     1981 agagtggacg aacaagccgc gagcaaacgg ttagcgcacg cgccccttgg tccctttccc
     2041 cgcctcacga cgagtggctg gcacggtcga tcccgccgtg gggagccgga gcgggtggtt
     2101 ggacagcagc cgctggttat agtcggcgcc attgatcaag agcgaaggct tgttccggcc
     2161 cgtggccggg gcctcgagcc ggtttccctg cgcgcgc
//
LOCUS       DATAQL010000780         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_384574, whole genome shotgun sequence.
ACCESSION   DATAQL010000780 DATAQL010000000
VERSION     DATAQL010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384574"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VHS97_13605"
     CDS             complement(<1..225)
                     /locus_tag="VHS97_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449290.1"
                     /translation="MKRGKAAAAEQAKTTAVFPPKTVGNRLLRWAVNFWVVFHFAAVI
                     AAAGSVGPTSDLVLAGWRLFRPYLQFFYLNH"
     gene            complement(230..421)
                     /locus_tag="VHS97_13610"
     CDS             complement(230..421)
                     /locus_tag="VHS97_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449291.1"
                     /translation="MFDLIRASHYTPFLHEKNERFGTCDFCTVLSRFGRFHVEIARLD
                     IPAAKVQDDRDIGVISAGG"
     gene            448..>3334
                     /locus_tag="VHS97_13615"
     CDS             448..>3334
                     /locus_tag="VHS97_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449292.1"
                     /translation="MTLGTQAQPHGKLLDYEQFIDHQLERTRSRIKFTDIMTAGLTLL
                     VGFLGVLFLEVVLDHMVGLPLLTRRLVLSAGVTIAAAFAAIRIAMPLLRRINGIYAAK
                     TIEDADPAFKNSLINYLELRRYRGQMPKAIMATLQARAVTDLTQVEIDNVVNQQRMLR
                     AFYTLSAVTVIFSLYALFSPKSILDSTRRAFLADVVRPTNTQLVNIKPGNDPKLAEIV
                     AGDHAIFSVHVEGVRPQKVLLHYSVDGGKFFAIREFAPGRLMYDPWQITLTNMQQTVE
                     YYLTGGDAESLHYRLKVLPAPTITSMELDLTFPSYTQVPPRSGIEGGTVEAIEGTDVT
                     VHARTNMPASLATLDISNETPAPMEIASDDPTVLTGRFKVKTSGTYKVNFRTTGNQLN
                     PNPVVYDIIAIPDRPPTARFVQPDKPSVKVPANVNVDLEMTGNDDHGVKDATLHVAMG
                     NEKLVSKNMLEGRPPQSEFKAIETFDLAKNRVKPGSTPSYWLTVRDNKEPSSNRTETA
                     RQIIEVTEPVSAPEKKKIEDSQQKDREQLDPTASSSADEESSADKPPPPESGKNGGEN
                     NDSGKGAPADDANQKPKDNGAQGTAGTGSADESNPDNKPGGPNNNSPQLSPDDQRIAD
                     KLKQALNNKKASNPPGDQANPANTQLKDGNSPPGNPRNADASRNNGKSQGGAQDSGKA
                     RGNDSQRPATEDTGQPNSRTAPSRPGDASSNDSSNQVQRADRSDNQPPSAAGRQGNDN
                     PGATPNQPADREQAKSKGDSNNPLDTTRQGRTPGDSRANDGTNPRSPQTAGNDSKGNR
                     TKSANSGDQNAGEKSGDQNADNNSNAAPGESTQSRDSTSGKSANRNGDPANKPNQGER
                     QDPSSAAKTSESQKNKTGHAQNENGSPAGGANKDGAKEQGNNSSSTAKTDDGRTNTPK
                     RDATNQKGDPSAGTDNDATKRQGSDPNSSTKTDNRQKSSKSGDSTKQD"
BASE COUNT          862 a         1067 c          911 g          494 t
ORIGIN      
        1 gtgattcaaa taaaagaact gtagatacgg tcggaagagc ctccatccgg ccaagaccag
       61 gtcggacgtc ggaccgaccg aacctgcggc ggcaatgacc gcggcgaagt ggaacacgac
      121 ccagaaattg accgcccagc gcaagagcct gttgccgacc gttttgggcg ggaacaccgc
      181 cgtggttttt gcctgctcgg ccgccgccgc tttcccgcgt ttcattgagt taccctccgg
      241 cggaaataac gccaatatcc cggtcatctt gcacttttgc ggccgggatg tcgagccggg
      301 caatctcgac atggaagcgg ccaaagcggc tcaagacggt gcagaaatcg cacgttccaa
      361 atcgttcgtt cttttcatgc agaaaaggcg tataatggga ggcacgaatc aagtcgaaca
      421 tcgagatccc cgcgaggagt agttgacatg actctcggga cgcaagcgca accgcacggc
      481 aagctactgg attacgagca attcatcgat caccagcttg agcggacgcg gtctcgcatc
      541 aagttcaccg atatcatgac ggcgggcttg acgctgttag taggcttcct gggagttctg
      601 tttcttgaag tcgtgctcga ccatatggtc ggtctgccgc ttctcacgcg tcggctcgtg
      661 ctgtccgccg gagtgacgat agccgcggcg tttgccgcga ttcggatcgc gatgccgcta
      721 ttgcggcgga tcaacggcat ctacgcggcc aagaccatcg aagatgccga tccggcgttc
      781 aaaaacagcc tcatcaacta tctcgagctc cggcgctacc gcggccagat gccgaaggcc
      841 atcatggcga cgctccaggc ccgagccgtc accgacttga cccaggtcga gatcgacaac
      901 gtcgtcaatc aacaacgcat gctacgtgcc ttctatacgc tctcggccgt gaccgtgatc
      961 ttttcgctat atgccctttt ttcccccaag agcatcctcg actcgacccg gcgcgccttt
     1021 ctcgccgacg tagttcgacc caccaacacc cagcttgtca acatcaagcc aggcaatgac
     1081 cccaagctgg cggaaattgt cgccggcgat cacgccattt tctctgttca tgtcgagggt
     1141 gtccgacctc agaaggtttt gctgcattac agcgtcgacg gcggcaaatt cttcgccatc
     1201 cgcgagttcg cccccggacg cctgatgtat gatccctggc agatcacgct cacaaacatg
     1261 cagcagaccg tggagtatta tctgacgggt ggcgacgcgg aatcgttgca ttatcggctc
     1321 aaagtcttac ccgcaccaac gattacgtcg atggagctcg acctgacgtt cccatcgtac
     1381 acccaggtcc cacctcggag cggtatcgaa gggggaactg tcgaggccat cgaggggact
     1441 gatgtcacgg tgcacgcccg gaccaatatg cccgcgagcc ttgcgactct tgatatctcc
     1501 aacgaaacac cggccccgat ggaaatcgcc tccgacgatc ccacggtctt gaccggcaga
     1561 ttcaaagtga agacgagcgg tacctacaag gtcaatttcc ggaccaccgg caatcagctc
     1621 aaccccaacc ccgttgttta tgacatcatc gccattcccg atcgaccacc caccgcccga
     1681 tttgtccagc cagacaagcc gtccgtcaag gtacccgcca acgtcaacgt cgacctggaa
     1741 atgaccggca acgacgacca cggtgtcaag gatgccactc ttcacgtcgc aatgggcaac
     1801 gagaaactcg tttccaagaa catgctcgag ggtcggccgc cccagtcgga attcaaggcg
     1861 atcgagacat tcgacctggc caagaatcga gtgaaaccgg gttcgacgcc cagctactgg
     1921 ctcacggtgc gagacaataa agagccgtcg tcgaaccgga cggagaccgc ccgtcagatc
     1981 atcgaggtta ccgagcccgt ctcggcgccg gaaaaaaaga agatcgaaga cagccagcag
     2041 aaggatcgtg aacaactcga tcccacggcg tcgtcaagcg ccgatgagga atcctcggcc
     2101 gacaaaccac caccacccga gtcgggtaag aacggcggcg aaaacaatga ctcgggcaag
     2161 ggcgcacccg cggacgacgc gaaccaaaag cccaaggaca acggggccca aggaacagcg
     2221 ggtaccggca gcgccgacga gtcgaacccc gataacaaac ctggcggccc gaacaacaat
     2281 tcgccccaac tttcgcccga cgaccagcgg atcgcggaca agctcaagca ggccttgaac
     2341 aacaaaaaag cctccaatcc gcccggcgat caggcgaatc cggccaacac gcaactgaaa
     2401 gacggcaatt cgccgccagg aaatccaagg aacgcggacg catcacggaa caacggcaaa
     2461 tcccaaggcg gggcccaaga ttccggcaaa gctcgcggca atgattcgca acgaccggcc
     2521 acggaagaca ccgggcagcc gaattcccga acggcacctt cacgccccgg agatgccagc
     2581 tcgaatgact cgtcgaatca ggttcagcga gctgaccgca gcgacaatca gcccccgagc
     2641 gcagccgggc gccaagggaa tgacaacccc ggtgcgactc ccaatcaacc cgcggatcga
     2701 gagcaggcca aatcgaaggg cgacagcaac aacccgctcg acacgacccg gcaaggtcga
     2761 acgcctgggg actcgcgcgc taatgacggc acgaatcctc gttcgccgca gacggccggc
     2821 aacgacagca aaggaaatag aacgaagtcc gcaaacagcg gagatcagaa cgccggcgaa
     2881 aaaagcggcg accaaaacgc ggacaacaat tccaatgctg cacccggcga aagcacccag
     2941 tcccgcgact cgaccagcgg gaaatccgcc aatcggaacg gggaccccgc aaacaagccg
     3001 aaccaagggg aacgccagga tccgtcttca gccgccaaga cgagcgaatc acaaaagaac
     3061 aagacaggcc acgcccaaaa tgagaatggc agtcccgcgg gtggtgctaa taaagacgga
     3121 gccaaggagc agggcaacaa ctcgtcctcg accgcgaaga ccgacgacgg aaggaccaac
     3181 acgccaaagc gtgacgccac caaccagaag ggcgacccgt cagccggaac ggacaacgat
     3241 gccaccaaga ggcaggggag cgaccccaac tcgtccacca aaaccgacaa ccggcaaaag
     3301 agttcgaaga gcggtgacag caccaagcaa gacg
//
LOCUS       DATAQL010000781         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2981211, whole genome shotgun sequence.
ACCESSION   DATAQL010000781 DATAQL010000000
VERSION     DATAQL010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2981211"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VHS97_13620"
     CDS             complement(<1..1044)
                     /locus_tag="VHS97_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3449293.1"
                     /translation="MDDEIFSAAVLSKLPLADATWRILHFTLADSWLQDLWNRERGRC
                     YEKVLEFSTLARLVAEALTQHGGSGRQAFERGQESDTLPVAISSAFDKLGNLPLPLSE
                     KLLEEGTQRLVEILPAGSAVDPFPLPGCWEGHEVFGVDGKAIKHVKRLLKPLRGLQAG
                     ILGARSSVALNLRTGMAVGMIGDLDGEAGEGALTERLLSNMVAAAGGKPWVAVLDRLY
                     CNLSFPHLVLAAGGHFLIRYCSNTHFVADPARPAQESRDARGQRIVQEWGRLGKSAGP
                     RALYVRRITLHLAGGTVISVITDLLDETAYPAEDLLATYRKRWGIERVFHQITDVFSL
                     KNLIGSPPRAVLFQ"
     gene            1298..>2987
                     /locus_tag="VHS97_13625"
     CDS             1298..>2987
                     /locus_tag="VHS97_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449294.1"
                     /translation="MSAHHADSGVAVRHIGDQLHLSWPLADGTGQLDLDLRPGQPLIR
                     TMGIAGKAGETPHPLLENADPVTFLLVGSRQAPADRPPGMSVFNVFFDSPATRPFQVY
                     RTHIELKRIRVGSQGHRATVSIGDVTVGPFTGELRFTLYRGAALVHVETVVHTSEDRR
                     AILYDTGLALPAAAKTRFAWVDTEGNLRREEIGPESSDRHLAVRHRVLIAESASGSVA
                     CFPPPHQFFSPRDLTDNLSTVWFGREHRGRDPRFGFGIRQSERGGGSWVPWFNAPPGT
                     DQRLGVFYLLSTGDAERSLRETLRYTNDDRFPKLPGYHTMTSHWHMATAMTALQEKAR
                     GITRSTPDVIGMFKAMGVEIVHLAEFHGDGHPQDPGPLRLPEMESMFAECRRLSDGNI
                     LVLPGEEANTGLGTARPGKEAGHWMYLFPRPVYWTMKRAPGQPFEENLAPFGTIYHVG
                     NGSDMVRLLEREHGLAWSSHARIKGSSWTPDFFRGQDFYRSSRWLGAAWKAMPADLSR
                     DRLGSRALDLLDDMANWGQEKYLPGEVDVFKIDHTHELYGHMNINYIRLEPDRVP"
BASE COUNT          552 a          988 c          896 g          551 t
ORIGIN      
        1 ctggaacagc accgcccgcg gcggcgagcc gatcaggttc ttgagcgaaa atacgtcggt
       61 aatttggtga aagacccttt cgattcccca cctctttcgg tacgtcgcca gcagatcctc
      121 ggcgggatac gcggtctcgt ccaacaggtc tgtgatcacg ctgatcaccg tgccaccggc
      181 caggtgcaag gtgatccggc gcacgtaaag agcccgaggt cccgcggact tgcccaaccg
      241 tccccactcc tgcacgatcc gttggccccg tgcatcgcgg ctctcctgag ctggtcgggc
      301 tgggtcggcg acgaagtgag tattggagca atagcgaatg aggaagtgac caccggccgc
      361 caaaaccaag tgcgggaagc tcaggttgca atagagccga tccaacaccg ccacccacgg
      421 cttgccgccg gccgccgcga ccatgttcga caagagcctt tcggtgaggg ctccctctcc
      481 ggcctccccg tcgaggtctc cgatcatgcc caccgccatg ccggtccgca agttcaaggc
      541 caccgacgac cgcgcgccga gaatgccggc ctgcagaccg cgcaacggtt tcaacagccg
      601 cttgacgtgt ttgatcgcct tgccgtcgac gccgaagact tcgtggccct cccaacatcc
      661 cgggagcgga aacgggtcga cagccgaccc tgcgggcaag atctccacca gtcgctgagt
      721 cccttcctcg agcagcttct cgctgagggg cagcggaagg tttccgagct tgtcgaaagc
      781 cgagctgatc gcgaccggca gggtatccga ctcctgccca cgctcgaagg cttggcggcc
      841 actcccaccg tgttgggtca gcgcttcggc aaccaggcgg gccagagtgc tgaactcgag
      901 gaccttttcg tagcagcgtc cccgctcgcg attccacagg tcctgcagcc aggagtcggc
      961 caaggtgaaa tgaagaatac gccaagtcgc gtcggcgagc ggaagcttcg acagaaccgc
     1021 tgcggagaag atttcgtcgt ccatgacgat ggctcctcag aagacccaag ggattatcag
     1081 gagatgactt gtgatatcct ttcttcatcg tcatgggaat cccaaaaacc cggacagcac
     1141 tgccttcaca agggggggaa agtgttgcct tcgtcactgg cccagggcca gggtggggat
     1201 cgatcgcatt gcccgatgtt tcgcattcac ggtcgcggtg tgctcctgct tttgccacgc
     1261 ggtcagatcc gcggtcgccg cggatcccat tgtcgacgtg tcggcccacc acgctgacag
     1321 cggcgtcgcc gtgcgccaca tcggcgatca gcttcacttg tcctggccac tcgcagatgg
     1381 caccggccag ctcgacctcg acctgcggcc gggccagccg ttgatccgca caatgggtat
     1441 tgccggcaag gccggtgaga ccccacatcc cttgcttgaa aacgccgacc cggtgacctt
     1501 tctgctcgtc ggttcgcgtc aagcacctgc ggaccggccg ccaggtatga gcgtcttcaa
     1561 tgtttttttc gactcgccgg ccaccagacc gttccaggtc tatcggacac atatcgagct
     1621 caagcgcatc cgcgttggca gtcagggcca ccgcgccacc gtctcgatcg gcgatgttac
     1681 tgtcggaccg ttcacgggcg agctacgatt cacactctat cgtggcgcgg ccttggtcca
     1741 cgtcgagact gtcgttcaca cctcagaaga ccgtcgtgcg atcctctatg acacaggtct
     1801 ggcactgccg gccgccgcca agacacgctt cgcctgggtg gacaccgagg ggaatctccg
     1861 gcgcgaggag atcgggcccg agtcatcgga tcgtcatctc gcagtccgtc accgcgtact
     1921 catcgccgaa agtgcgtcgg gctcggtggc gtgctttccg cctccgcacc agttcttttc
     1981 gccgcgcgac ctgaccgaca acctcagcac ggtctggttc ggtcgcgaac accgtggcag
     2041 ggacccgcgt ttcggattcg gcatccgaca gtccgagcga ggaggcggtt cctgggtccc
     2101 ctggttcaac gccccgcccg gcaccgacca gcgcctgggg gttttctatc tcctctcgac
     2161 cggtgacgcc gagcgctcgt tgcgtgagac gttgcgttac accaacgacg atcgcttccc
     2221 caaactccct ggctatcaca cgatgaccag ccactggcac atggcgaccg ccatgaccgc
     2281 cctccaggag aaggcgcggg ggatcacccg ctcgacaccc gatgtgatcg gcatgttcaa
     2341 ggcgatgggg gtggagatcg tgcacctggc cgagtttcac ggcgacgggc atccccagga
     2401 tccgggaccg ttgcgcctgc ccgagatgga atcgatgttc gccgaatgca ggcggctatc
     2461 cgacggcaat atcctggtcc ttcccggtga agaggcgaat accggcctgg gcacggctcg
     2521 gcctggcaaa gaggcgggcc actggatgta tctttttccc aggccggttt attggacgat
     2581 gaaacgcgcg ccaggccagc cgtttgaaga aaaccttgct ccattcggaa cgatttatca
     2641 cgtcggcaac ggatcggata tggtacgact tctcgagcgc gaacacggcc tggcgtggag
     2701 ctctcacgcc cggatcaagg ggtcgagctg gacccccgac tttttccgcg gccaggactt
     2761 ttaccggtcg agccgctggc tgggggccgc gtggaaggcc atgccggccg acctctcgcg
     2821 cgaccgattg gggtcgcgcg ccctcgactt actcgacgac atggccaact ggggacagga
     2881 aaagtacctc cccggagagg tcgatgtctt caagatcgac cacacgcacg agctctacgg
     2941 ccacatgaat atcaactaca tccggctcga gcccgaccgg gtgcctc
//
LOCUS       DATAQL010000782         7931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2217761, whole genome shotgun sequence.
ACCESSION   DATAQL010000782 DATAQL010000000
VERSION     DATAQL010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217761"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="VHS97_13630"
     CDS             <1..1606
                     /locus_tag="VHS97_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX3449295.1"
                     /translation="SAADYYVSPAGDDAGAGTATAPWRSIDRVNGHALRPGDRMLFQG
                     GGEFAGNLVLASPGSPTDAANPIVVGSYGRGRAVIRAGRGTGIKVEDLGGVVVRDLVV
                     VGDPRVGNEGFGVLVLHRRPDPERLEGVRIDDVEARGFRWAGIYVGGVPTDLPGFDAP
                     MESRLGFSGVRIRRCVARNNVYHGIYVDGAAKGSGANGYANRDVVIVDCVAADNPGDP
                     HYTAKHSGNGILIGDTDEGLIEHCVAHGNGAANAGREGGPVGIWTYASNRVTIRSSES
                     YGNRTGGHADGGGFDLDGGVTNSVIEYCYSHDNDGPGFLIWNYEGAPHRLAGNILRYN
                     ISAVDSRKHRYGGISVGTSDAPIHDLLVHNNTVFATRVGVDEPSCVRVWEHSGDGLRF
                     VNNLFVTSGGVPAIACEMRGDGVCFVGNAYWAADGAFQIRHGERFDDLTAWRAATGQE
                     RWRGRDVGIYADPRLDGASTSGAGQPPSPLGQFRLRTGSPLIDAGLDLVGFSDHGPGD
                     RDFRGTTIPKGRGPDVGANEFDPSGR"
     gene            complement(2397..2912)
                     /locus_tag="VHS97_13635"
     CDS             complement(2397..2912)
                     /locus_tag="VHS97_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449296.1"
                     /translation="MATATARTVGKTEFVKEVLGKNPHANPTAVNEEWKSAGQDGTIS
                     ATLVNKLRSSMGLAGNLRPTPKTKTRSRSVENRPPSGKHRGEKLKSSSSETVPPAPSR
                     SNGKKTDQAAMRDERRAKVNSHHGSLEELECDIDRLLFKVMELGGLPAIEDSLRQTRR
                     LLYGGFSGKRS"
     gene            complement(3024..3509)
                     /locus_tag="VHS97_13640"
     CDS             complement(3024..3509)
                     /locus_tag="VHS97_13640"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEX3449297.1"
                     /translation="MPPRSSSGYYGFKVIGTLKLLSGLVAVVVGIGTVRFLDHDPGPK
                     LERAVIHLGLDPHNEVIHSAISRITGVDRTHLRAIQAGTFFYALLHLVEGIGLILEQD
                     WAGYLVVIATSSLIPFEIYEIAKKPSRTRIALFVLNVGIVIYLIATLKRERANRARQP
                     V"
     gene            complement(3534..4331)
                     /locus_tag="VHS97_13645"
     CDS             complement(3534..4331)
                     /locus_tag="VHS97_13645"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX3449298.1"
                     /translation="MKTKSEIVKNWLPRYTGRPLADFGKFVLLSNFTSYVDMFADQFQ
                     VEVIGRSHPMQSATAEDLTILNFGMGSAMAATVMDLLSAIEPRAVLFLGKCGGLKKSK
                     VGDFILPIAAIRGEGASNDYMPPEIPALPSFRLQKAVSATIVKQGFDYWTGTVYTTNR
                     RVWEHDEKFKEYLTSIRALAIDMETATIFIVGFVNHIPKGALLLVSDSPMTPEGVKTG
                     RSDSSVNERYVRKHLEIGIASLIELRDSGESVKHLRFESSGDDKRRR"
     gene            complement(4369..5085)
                     /locus_tag="VHS97_13650"
     CDS             complement(4369..5085)
                     /locus_tag="VHS97_13650"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX3449299.1"
                     /translation="MCLVFISIQTDGKGPVMIGANREESRLRPTTSPVCCRRRGLRCL
                     LAGADHGPDGTFPEMGTWLGVNEAGMAIAVTNRHDGELAWADQTRSRGLLAVELIGNV
                     QPERAIEAAKHELARGGYGGCNFLIANRDSAFVIEAPGPARISVCRLQSGIHAMTNLD
                     LDDQADARIQLVRTTLDALNFAESARRLCRDERIVVSGAKHGTISSSVILASHDVVLF
                     HKDGDSRTVDYQEFRLLDPD"
     gene            5244..6443
                     /locus_tag="VHS97_13655"
     CDS             5244..6443
                     /locus_tag="VHS97_13655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449300.1"
                     /translation="MNKEAIALLSLGHFLIDFCQGVVPALVPFLVTERHFSYTAAASL
                     VFAISATSSVVQPLFGQLADRLALAWLLPASVLLAGLALALGSQSPSFAVVLTAFGLS
                     GLGVAAFHPEAARKAYLASGERRTTGMSLFSVGGGLGFALSPALTTAVVVTWGISGLL
                     ALLAPAAIIAALLARKLGEASASSQLHEKGGLHWQREDDWRGFGILSGATISRSIVFY
                     GLNTFLALYFMTRWHQTAAQGNKALVIFLGTSIVGTLLGGWLADRVGRRAVIRSGFVG
                     AAVFLGLFVQTQDRDWALAFLIPLAVFIFLPTSVLVVLGQEYLPRRVGMASGVTLGLA
                     VSVGGMCAPFLGRLADWKGLGYVLVLLLAVLLVATFQAFLLPPVRGSEASELEFPISP
                     VTADRSE"
     gene            6471..6980
                     /locus_tag="VHS97_13660"
     CDS             6471..6980
                     /locus_tag="VHS97_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3449301.1"
                     /translation="MNVKLCLISSGVLAMAALVNPASTQGQVDREDSIRAVRMVLDRQ
                     VADWNKGDLDAFLTGYWNSPKVVFQSGGQRHVGWSAMRERYRRRYQGEGKAMGQLEFS
                     GLEIEPLGQDSIMARGGWRLTMPDGTKPGGLFTLIFRRLPEGWRIVHDHTSAEETAPR
                     KPSSAAASQ"
     gene            complement(7027..7608)
                     /locus_tag="VHS97_13665"
     CDS             complement(7027..7608)
                     /locus_tag="VHS97_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449302.1"
                     /translation="MWGKVVIGGLVAGIVVFFWGAIAHVALPLGEMGIRTIPDEHAVL
                     GAMKNAIREPGFYFFPGHDMSKPLSESEQKAFDDKIKQGPTGVLVIHPEGGEAMSPRQ
                     LLTELASNVAAALLAALVLTQVRSGYLGRLLVVTLMGVFGFVSISVSYWNWYGFPLDF
                     STGEAIMEIVGWFLAGLVLAAIVRPRVAAPVAA"
BASE COUNT         1510 a         2341 c         2450 g         1630 t
ORIGIN      
        1 ctcggccgcg gactactacg tcagccccgc cggcgacgac gcgggcgccg gtactgcgac
       61 tgcgccctgg cgcagcatcg atcgggtcaa cggccacgcg ttgcgccccg gcgaccgtat
      121 gctctttcag gggggcggcg agttcgccgg caacctcgtg ctggcgagcc cgggatcgcc
      181 gaccgacgca gcaaacccaa tcgtcgtcgg cagctacggt cggggccggg ccgtgatccg
      241 cgccgggcgg ggcacgggca tcaaggtcga ggatctcggc ggggttgtgg tccgcgacct
      301 ggtcgtcgtc ggcgatccac gcgtcggcaa cgaagggttc ggcgtcctgg tgcttcaccg
      361 gcggcccgac ccggagcggc tggagggcgt ccggatcgac gacgtcgaag cacgcggctt
      421 ccgctgggcc ggcatctacg tcggcggcgt ccccaccgac ctgccgggtt tcgacgcgcc
      481 aatggagagc cgtttaggtt tctccggcgt gcgcatccgt cgctgcgtgg cccgcaataa
      541 tgtctatcac gggatctatg tcgacggggc agcgaagggg tcgggggcga acggctacgc
      601 gaatcgggat gtcgtcatcg tcgactgcgt cgccgccgac aacccaggcg acccgcacta
      661 tacggccaag cacagcggca acggaattct gatcggcgac accgacgagg gcctgatcga
      721 gcattgcgtt gcccacggca acggggcggc caacgccggc cgggagggcg ggccggtcgg
      781 gatctggacc tatgcctcga accgcgtcac catccggtcg tccgaatcgt atggcaaccg
      841 caccggtggc catgccgacg gcggcggttt cgacctcgac ggcggcgtga ccaactcggt
      901 catcgagtac tgctacagcc acgacaacga cgggcccggc ttcctgatct ggaattatga
      961 gggcgcgccc catcggctgg ccggcaacat cctccggtac aacattagcg cagtcgacag
     1021 ccgcaagcac cgttatgggg gcatcagcgt cggcacttcc gatgcaccga tccacgacct
     1081 gctggtccac aacaacacgg tcttcgcgac gcgcgttggc gtcgacgaac cgtcgtgcgt
     1141 ccgtgtctgg gagcattccg gcgatggcct ccggttcgtg aacaacctgt tcgtcaccag
     1201 cggcggcgtg ccggcgatcg cctgcgagat gcgcggcgac ggggtctgct tcgtgggcaa
     1261 cgcctactgg gccgccgacg gcgcgtttca gatccgccac ggcgaacggt tcgatgatct
     1321 cacggcctgg cgcgcggcga ccggccagga gcgatggcgg ggcagggacg tggggatcta
     1381 cgccgacccg cggctcgacg gcgcgtcgac aagcggggcc ggccagccac cgtcgcccct
     1441 cggccagttc cgcctccgta cgggatcgcc gctcatcgac gccggcctcg acctcgttgg
     1501 tttctcggat catggacctg gcgaccgcga cttccgcggt accaccatcc cgaagggccg
     1561 gggccccgac gtgggggcca acgagttcga tccgtcgggg agatgagccc ggctcgcagt
     1621 cgagagaggc accgacatcc cggacatcgt cgacgacacc gggggcagcg gaacctgaaa
     1681 gttggcctga ccccttggcc gcgaaggccg cgggcagccg ctgcttgtgc atgggactgc
     1741 ccgcgtactt cacggccgat cagctcacag ccgtccgtgg gattggacgg ctgcgaaccg
     1801 ggcgatggct cccgaaacgg tgcaaaaccg tatagcgtgt cgatcaccga atgcgagtgg
     1861 cggtccgcgg cgacaaatac ttgtacgcag cagaaagccg ccaggaaaac caagacgacc
     1921 gcttgccacg atgatggccg gtacagccat tcccacggtt tgaaccatac gcgagtcatg
     1981 gcaaaccacg atgtggatgc acaccgccgt gacgtttcgt gttccggaag cggccggcaa
     2041 ctgggggcga ggctatgatg gttcgccgag aatgccgagg ccggtcataa gaggacaacc
     2101 gccccggctg acggggttag ttaacccatc gacgggttct tgagcacatc agacgtcgac
     2161 aagctcgccc aaccttgcac tcccgccgta ctcccggctg ggctatcggc agtgtgccat
     2221 tgcgcgagcg ataaatctag aggagtaaac ttactaatgt acccacgttg ataaaatgcc
     2281 tcattattgt tgcaattctc caacaaaatg gaaccatgtc tcatatagcc gggggtgtta
     2341 ttcccgtgaa atcttatgtt catcggaaca ctacttgagc aagacattct gttgcctcag
     2401 gagcgcttcc cggaaaatcc accatagaga agccttcgtg tctgtctcaa gctatcctcg
     2461 atggcgggta gtccgcccag ttccatcacc ttgaacaaca gtcggtcgat gtcgcactcc
     2521 aattcttcca aggagccgtg atggctatta accttggcgc gacgttcatc tcgcatcgcc
     2581 gcttgatcgg tctttttgcc gttggagcgg gaaggggcag gcggtacggt ctcgctcgaa
     2641 ctgctcttga gcttttcccc tcgatgcttc ccggaaggag gccgattttc aacggagcgt
     2701 gatcgtgtct tggttttggg cgtagggcgt aggttacctg ctaaccccat ggaagacctt
     2761 agcttgttga ccaatgtcgc gctgatcgtt ccgtcctgac cggctgactt ccattcttcg
     2821 tttacggctg ttgggttggc gtgaggattt ttgccgagca cttccttgac gaattcagtc
     2881 ttgcctacgg tcctggctgt tgctgtggcc atgagcgttc tccagttcgt tgttttgtat
     2941 gaaagcgata gtatagtcga aaccgcgacc aggtacaaat tgtgcaatca catccgtctg
     3001 tatacgacag acacgtggac gggtcaaacg ggctgcctcg cccgattcgc ccgctctctc
     3061 ttcaaggtcg cgatcaggta gatgacgatg ccgacgttca agacgaaaag cgcgattctc
     3121 gtacgactgg gctttttggc gatctcatag atttcgaacg ggatcagcga actggtagcg
     3181 atgacgacca agtagccggc ccaatcctgt tcgagtatca acccaattcc ctcgaccaaa
     3241 tgcaaaagcg cgtaaaagaa ggttcctgct tggatggctc gaaggtgtgt tcgatcgact
     3301 cccgtgatcc gggatatcgc gctatggatg acttcgttgt gaggatcgag tccgaggtga
     3361 atgaccgctc gttccagttt tggtccaggg tcgtgatcga ggaagcgaac ggtcccgatg
     3421 ccgacgacca ctgcgaccaa gccggacagg agcttgagag tcccaatgac cttgaacccg
     3481 tagtagccgg aacttgaacg tgggggcatg ggtttcctcc gcgagaccgg cggtcaccgc
     3541 cttctcttat cgtcgccgct tgactcgaaa cgcaagtgct tgacagactc gcccgaatcg
     3601 cggagctcga tcaaggaagc aattcctatt tccaggtgtt tccgcacata gcgttcatta
     3661 acggaactgt cgctgcgacc ggtcttgact ccctcgggcg tcatcgggct gtccgatacc
     3721 agaagcagcg cacccttggg tatgtggttg acaaacccca cgatgaagat cgtcgctgtc
     3781 tccatatcga ttgctaaagc gcgaatggat gtgaggtact ctttgaattt ctcgtcgtgt
     3841 tcccacacac ggcgattggt cgtgtagact gtgccggtcc agtaatcgaa gccttgcttg
     3901 actatcgttg ccgagacggc cttttgcaac cggaacgatg gtagagcggg aatctccggc
     3961 ggcatgtagt cgttgcttgc cccttctccc ctgatggcgg cgatcggaag gatgaagtcg
     4021 ccgaccttcg acttcttcaa gcccccgcat tttcccagga agagtacagc cctgggctcg
     4081 attgccgaga gcagatccat cacggtcgcc gccatcgcgc ttcccatgcc gaaattgagg
     4141 atcgtcaagt cctccgcggt ggccgattgc atgggatgcg atcggccaat cacttcgacc
     4201 tgaaactgat ctgcaaacat gtcgacatag ctagtaaaat tggagagtaa gacgaacttg
     4261 ccgaagtccg cgagcgggcg accggtatac cgggggagcc agttcttgac gatctcggat
     4321 ttggtcttca cgacatcgat acctagtggc tgggattgga ctgcactctc agtcaggatc
     4381 aagtagtcga aattcctggt agtcaacggt tctcgagtcg ccgtctttat gaaagagcac
     4441 gacgtcgtgg ctggcgagaa tcacgcttga cgaaatcgtc ccgtgtttcg ccccggagac
     4501 gacaatcctt tcgtctcggc aaaggcgcct ggccgactcg gcgaagttta gagcgtcgag
     4561 agttgtccgc acgagttgaa ttcgagcgtc agcttgatcg tccaggtcga ggttagtcat
     4621 cgcgtgaatt ccggactgga gtctacagac agatatgcgc gccggtccag gggcctcgat
     4681 gacgaacgca ctatcgcggt tggcaatcag gaaattacag ccgccgtagc ctccccgcgc
     4741 cagctcgtgc ttggcggcct ctatagcgcg ttcaggttga acgttgccga tcaactccac
     4801 ggcgagtaat ccgcgggaac gagtctggtc ggcccaggcg agctcgccat catgccggtt
     4861 tgtgaccgcg atcgccatgc cggcctcgtt aacgcccaac caggtgccca tctcggggaa
     4921 cgttccatcc gggccatgat cggcaccggc cagcagacaa cgcaaccctc ttcgtctgca
     4981 acaaacgggc gacgtcgtcg ggcggaggcg cgattcttct cggttggcac cgatcatcac
     5041 ggggcctttg ccgtcggtct ggatgcttat gaagacgaga cacatgcggc tgcatccctc
     5101 gaggaagtgg aaccccgtcg tttgtagtcg ggagggcggt ttcctgccaa gtcgtttgaa
     5161 gaaggaccgt tcattgtaca tgatggcaaa gcctggaccg aatttgttgg gagcctcggt
     5221 acgcacttat tgaggatagg ctgatgaaca aagaggcgat cgcgctcttg tcgttggggc
     5281 acttcctgat cgacttttgc cagggcgtgg tgccggcact ggtgccgttt ttggtgacgg
     5341 aacgccattt ctcatatacg gcggcggcga gtttggtctt cgccatcagc gcgacttcct
     5401 cggtggtcca gccccttttt gggcagcttg cggatcgcct ggcgctggca tggctcttgc
     5461 cggcaagtgt gctcttggcg gggctggctc tggcgttagg atcacagtcg ccaagttttg
     5521 ctgtggtatt gacggctttt ggactcagcg gccttggggt ggccgccttt caccccgagg
     5581 cagcccggaa ggcctacctc gccagcggcg aacgccggac gaccggcatg agcctgttct
     5641 cggtcggcgg tgggctcggg tttgccctat cgcctgcgct cacgactgcg gtggttgtca
     5701 cttggggcat atcggggttg ctagcgcttt tggcgcccgc cgcgatcatc gcggccttgc
     5761 tggctcgaaa gctcggcgag gcgtctgcgt cgtctcaatt gcacgagaag ggcgggcttc
     5821 attggcaacg tgaagacgac tggcgcggat ttggcatcct gagtggagcg acgattagcc
     5881 ggtcgatcgt tttctacgga ctgaacacat ttctggcgct ctacttcatg acccgctggc
     5941 accagaccgc ggctcaagga aacaaggcgc tggtgatctt tctgggtacc agcatcgtcg
     6001 gtacacttct ggggggctgg ctggccgacc gcgtcggtcg gcgggcggtg attcgtagcg
     6061 gctttgtggg ggccgcggtg tttctgggtc tgttcgtgca aacgcaggac cgggattggg
     6121 ccttggcgtt tttgattccg ctggccgttt ttatcttctt gccgacgagc gtgctcgtcg
     6181 tgctcggtca ggaatacctg ccgcggcgcg tcggaatggc ctccggggta acgctgggat
     6241 tggccgtgag cgttgggggt atgtgcgcac cctttctggg tcgcctcgcc gattggaagg
     6301 gtctgggcta cgttctcgtt ttgctcttgg ccgtgttgct ggtggcgacc tttcaggctt
     6361 ttctgttgcc gccggttcgt ggtagcgagg catccgagct agagttcccg atctcgccgg
     6421 tgacagcgga tcgatcagaa taacaacatc agttcgagga gacttgcagc atgaatgtca
     6481 aattatgttt gatcagctct ggcgtcctgg caatggcggc gctcgtgaat ccagcatcga
     6541 ctcagggaca agtcgatcgc gaagattcca tccgggcagt ccgcatggta ctggatcgcc
     6601 aggtcgccga ctggaacaag ggcgatcttg acgcgtttct gactggttac tggaattcgc
     6661 cgaaggtggt ttttcagtcg ggcggtcagc gtcacgttgg gtggagcgcg atgcgtgagc
     6721 ggtatcgtcg ccgctatcaa ggcgagggca aggcgatggg ccagctcgaa ttctccgggc
     6781 tcgagatcga gccgctcggc caggattcga tcatggcgcg agggggctgg cgactgacga
     6841 tgcccgatgg caccaagcct ggaggacttt ttaccctgat ctttcggagg cttcccgaag
     6901 gatggaggat cgttcacgac cacacttcgg ccgaagaaac cgcgcccaga aagccgtcaa
     6961 gcgcggcggc aagtcaatga ggctccgaat atagtcgcga cccaatgcat cgagcctgcc
     7021 attagattag gcagcgaccg gcgccgccac gcgaggccgg acgattgccg ccagcacgag
     7081 gcccgcgaga aaccaaccga cgatttccat gatcgcttcg cccgtgctga agtcgagagg
     7141 gaagccgtac cagttccagt aggaaacgct gatgctcaca aatccgaaca cacccatcaa
     7201 cgtcaccacg agcagacgcc ccaggtatcc tgagcgaacc tgagtcaaca ccagcgcggc
     7261 cagcagagct gctgccacat tactggcgag ttccgtcagc aactggcggg gcgacatcgc
     7321 ctcgccccct tcggggtgga ttaccaaaac cccggttggg ccttgcttga tcttgtcatc
     7381 gaaagctttt tgttccgatt ccgacagagg cttgctcatg tcgtggcccg gaaagaaata
     7441 aaaacccggc tcacgaatcg catttttcat cgcgcccaag accgcgtgct catcgggaat
     7501 tgtgcgaatg cccatttcgc cgagcggcag cgccacgtgc gcgatcgctc cccagaagaa
     7561 caccacaatt cccgcgacga gcccaccgat tacaaccttg ccccacatgg ccgccccctt
     7621 tgcaccggaa gccaatcaga ctgtaggcgc cactaaacgg ccttgcgtga ttccgggagg
     7681 gcgaggcacc gtccgagccg gattgcgacg aggctcgccg ggaggcgcgc cctcccaggt
     7741 tcgtgcaatg gcatttagga cctgatcgta atgggagtat ggccctcgat caataacaat
     7801 gtttgaatct cacggaaaca tcgagctctc tggagctcat ctccgccaaa gtgcccagac
     7861 catcaagcac ccgatcgaga cgaaaacgac ggggagaaac cacttgtcaa agaatttctg
     7921 catgggagca a
//
LOCUS       DATAQL010000783         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1454565, whole genome shotgun sequence.
ACCESSION   DATAQL010000783 DATAQL010000000
VERSION     DATAQL010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454565"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..723
                     /locus_tag="VHS97_13670"
     CDS             118..723
                     /locus_tag="VHS97_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449303.1"
                     /translation="MPREMGFDYSAMSFQEQKAAQGVPPAAIFGLSLLFVFLILAALY
                     ESWSLPFSVLLGTPVAVFGAYLTLWLRAFENNVYAQIGLVMLIGLAAKNAILIVEFAK
                     SEYENGRSIEDAALTGARIRLRPILMTAFAFILGCVPLWVAAGSGAVSRQVLGTAVIG
                     GMLAASLIAIFFIPVTFDVVERISHALGRAPAPAPQQQEGD"
     gene            720..>1792
                     /locus_tag="VHS97_13675"
     CDS             720..>1792
                     /locus_tag="VHS97_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3449304.1"
                     /translation="MRTAVVITVLLGLAGCTVGPNYRRPAVAVPETYRNAEPPSGTAS
                     LGSEKWWDIYQDPKLEDLIHTALARNYDVRIAATRILQAQAQLGITRSEQFPQGSGSA
                     GYSGQKIPLFGFTSFQLQGIFSWNIDFWGQYRRATEAARANLLATDWNRNLVLSTVVA
                     NVATAYFQLRELDLELDIAKRTLASRRESLQLTTTLERGGATSLLDVRQAEQLVETAA
                     ETIPEAERQIAVFEDTLSTLIGDNPHGIERGLPLTEQKVPELIPAGLPSELIERRPDI
                     RDAEQQLIAANAQIGVARAQLFPTLPLTGAGGLESTNLSSLFSGTAWNFSAPLTQPIF
                     NAGRLRSNVKLAEAQQQQALLTY"
BASE COUNT          346 a          547 c          531 g          368 t
ORIGIN      
        1 ccggagttca ccatgcgcta caacgaatat cacgcggcgc agatcaacgc ttcggccgcg
       61 cccggctaca gttccggcca agccatgaaa gctctagagt aaacctttgc gcagaccatg
      121 ccgcgcgaaa tggggttcga ctatagcgcc atgtcgttcc aggaacagaa agcggctcag
      181 ggcgtgccgc cggccgccat tttcggcctc tccttgctgt ttgtatttct cattctggct
      241 gccctgtatg agagctggtc gttgccgttc agtgttctgc tcggaacgcc tgtggccgtc
      301 ttcggagctt atctgacttt atggctgcga gccttcgaaa acaatgtgta cgcccaaatc
      361 gggctcgtga tgttgatcgg actggcggcg aagaacgcga ttctgatcgt agaattcgcc
      421 aaatccgaat atgaaaacgg aaggagcatc gaggacgcgg ccttgaccgg cgcgcgcatc
      481 cggttgcggc ccattctgat gacggccttc gctttcatcc tcggctgtgt tcccctgtgg
      541 gtcgcggccg gatcgggcgc ggtctcgcgg caggtcctgg gcaccgcagt gatcggaggg
      601 atgctggccg cctccctcat tgccatcttt tttattcccg tgaccttcga tgtagtcgag
      661 cgaatttccc acgcgctcgg gcgcgccccc gctccggcgc ctcagcaaca ggagggagat
      721 tgagaaccgc ggttgtcatt acggtgcttc tcggcctcgc cggctgcacc gttggtccca
      781 actatcgccg ccccgccgtt gcggttccgg aaacctatcg gaacgcggag ccaccctccg
      841 gaacggcatc actcggcagc gaaaagtggt gggatattta tcaggacccg aaactggagg
      901 acctgattca taccgcgctg gcgcggaatt acgatgtgcg gatcgcggcc acccgtattt
      961 tgcaagcgca ggcgcagctc ggaatcacgc gatccgagca gttcccgcaa ggaagcggaa
     1021 gcgcgggtta tagcgggcag aagatccctc tattcggttt cacttcgttc cagctgcagg
     1081 gaatcttttc gtggaatatc gatttttggg gccaataccg gcgggcgacc gaggctgcgc
     1141 gcgccaatct gctggctacg gactggaacc gaaatctggt attgagcaca gtcgtggcca
     1201 atgtcgctac ggcttacttt cagcttcgcg agctcgacct ggagctcgat atcgccaaac
     1261 gcacgctcgc ttcgcggcgt gagtcgcttc agctcacgac tacgctggaa cgcggcggag
     1321 ccacgagcct tctcgatgtg cggcaggccg agcaactggt ggagacggcc gccgagacca
     1381 ttcctgaagc ggagcgccag attgctgtct tcgaagatac gctaagcacg ctaatcggcg
     1441 acaatcccca cgggatcgag cgcgggctgc ctcttacaga acagaaggtc ccggaattga
     1501 tccccgccgg gttgccctcg gagctgatcg agcgccggcc ggatatccgg gacgcggaac
     1561 aacagctgat cgctgccaac gcacaaatcg gtgtcgcccg tgctcagtta ttcccaacct
     1621 tgcccttgac gggcgccggc ggcctggaaa gcacgaacct gtcgagtttg ttcagcggaa
     1681 ccgcctggaa tttctccgcg ccgcttaccc agccgatctt taacgcagga cggcttcgct
     1741 cgaatgtgaa gttagccgaa gcacagcaac aacaggcgct gcttacctat ca
//
LOCUS       DATAQL010000784         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1606124, whole genome shotgun sequence.
ACCESSION   DATAQL010000784 DATAQL010000000
VERSION     DATAQL010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606124"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VHS97_13680"
     CDS             <1..275
                     /locus_tag="VHS97_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449305.1"
                     /translation="LGPTLPDQRERPIEIEKHVGDICAGLERSRQLDAGPVRMALTWL
                     GSSAHNQGRLAITAILTKLTTVGRDERNASIRPVPGMIFPSQWFSL"
     gene            complement(527..1192)
                     /locus_tag="VHS97_13685"
     CDS             complement(527..1192)
                     /locus_tag="VHS97_13685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX3449306.1"
                     /translation="MSLEQWSAVDRFITDLLVSPDVALESALADSAAAGLPAISVSPN
                     QGKLLHLLARAQGARSILEIGTLGGYSTIWLARALPDGGRLITLEFEPRHAEVARSNI
                     ARAGLADVVDIRLGPALETLPRLVADGLSPFDFVFIDADKENTADYFAWSLKLTRRGS
                     MIIVDNVVRKGAVINADSDDANVQGVRRFYEMLAAEPRVSATAIQTVGSKGYDGFAIA
                     LVN"
     gene            1863..3050
                     /locus_tag="VHS97_13690"
     CDS             1863..3050
                     /locus_tag="VHS97_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449307.1"
                     /translation="MAGWLAASGCLGPAAVRFTRMQYNEAVRSTNDEQLLTNLVRLRY
                     ADTPVFVDLPNITSQFELAAGGSDPGPSGSQTNFGVGGAWGRDSPTLSYHPRQGREIA
                     KALLDPLSPNLYSVVNAGARLDQLMWMTLNDINDVQNAARAATLVPRVPDDNTLFLRG
                     IQLLAEIDDRGGAEIGFSPIENKKSTSDPVLAGHVQGRDVLDAARGGYVYRDTGDGRM
                     ALYKRESVLTLKIRSPFTHSPEMEEMARIFNLTAGLPRYKIESELEPGAESTPPSALP
                     VGDTIYLNLRSILQIMTFLSKGVCIPDEHIRNGVAPMTPDADGRPFDWTRITAGNFVV
                     DVQKHRPRDAEVAVPYRGYWFYIRRGDVNSRSVLAVLEILFSLQESDEKSTGPVLTLP
                     AGR"
     gene            complement(3167..>3590)
                     /locus_tag="VHS97_13695"
     CDS             complement(3167..>3590)
                     /locus_tag="VHS97_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449308.1"
                     /translation="ILIVEFAKQSQEAGASRREATLQACRLRLRPILMTSFAFIFGVV
                     PLVIAEGAGAEMRRSLGTTVFAGMLGVTLFGIFLTPVFYYVVQWFGGTKEPEVRHKPD
                     GHPDRNGMAMLVGHAGHDSYMNSFAPQHAGSDGTVDPE"
BASE COUNT          701 a         1112 c         1122 g          655 t
ORIGIN      
        1 acctcgggcc aaccctgccg gatcaacgag agcgtcccat cgaaatcgaa aagcacgtgg
       61 gtgatatctg tgcggggctg gagcgcagcc ggcagctcga cgctggacca gtccgaatgg
      121 ccctcacctg gctcggcagc tccgctcata accagggccg cctggcgatc accgccattc
      181 tcaccaagct gacaacagtt ggccgggacg agcgaaacgc ttccatcaga cctgtaccgg
      241 gcatgatatt tccatctcaa tggttctcgc tctagacggc aagctggtcg acccgcgggg
      301 cagctcccag agccccttga tcttaacaca atttccatat ttggaaattg ggaattcgtc
      361 taatctttcg tcggctcttg ctttacggcc gggtcggtta tcacacgttt acagtcgcgg
      421 tagcgtcgcc gtgacgacct tcgcagcata ggtgcggaac gtacgacgaa tcgatccgag
      481 ctttgaaatt tgagacgaga agtcgcgcga cctcgatcgc ggcgcgtcaa ttgaccaggg
      541 cgatggcgaa gccgtcgtac cctttgctgc cgacggtctg gatcgccgtg gcgctgaccc
      601 ggggctcggc ggcgagcatc tcgtaaaacc ggcgcacccc ttggacgttg gcgtcgtcac
      661 tgtccgcatt gatcaccgcc cccttacgga cgacgttgtc gacaatgatc atgctccccc
      721 ggcgggtaag cttgagggac caggcgaagt aatcggcggt gttctccttg tcggcgtcaa
      781 tgaagacaaa gtcgaatggg ctcaggccat cggcgacgag tcgaggcagg gtttcaagtg
      841 ctgggccgag ccgaatatcg acaacgtctg caagtccagc acgcgcgatg ttggatctgg
      901 cgacctcggc gtgcctgggc tcgaactcga gtgtgatcag ccgcccaccg tcaggaagcg
      961 cacgcgccag ccagatggtg ctgtagccgc ctagcgtacc gatttcgaga atagaccgag
     1021 cgccttgcgc gcgggcgagg agatggagga gcttcccttg gttgggcgac acgctgattg
     1081 ccggcaaccc tgcggccgcg ctgtccgcca gcgccgactc cagggcaaca tcgggagata
     1141 cgagcagatc cgtgatgaag cgatcgacag ccgaccattg ctccaggctc atggcgaggt
     1201 ggcctcgtca aagatctgcg gaagacagga cttgaacctg cacgggcgaa tttagcccac
     1261 tagggcccga acacagtgtg gtaacaaatg tgtgacatgg gcaacacatc gcggtgacac
     1321 ttaaaccatt gatatacaag gctattacgt ctacgcctcg ctaagccgca ttgtagcggc
     1381 attgaattgg cgttgtcagt attgttgcca aattgttgcc agttcgctag ccagcctacg
     1441 taaccgaagg ccaagacccc gtcggcagtg gtaccgccta tgatggcagg aggcccaagg
     1501 tcggagaaat ccattcggga agacttctcg cgccttcgcc cggaccgcac gggacgattc
     1561 cgccgtacct tggaattgat ggagttcatt gggctccctt tcctggccag cgctgatccg
     1621 gatgatgttg acggaactca tccgattttt cagaccgggg accgtcggaa gtaggcccgt
     1681 agcgaccttg acattttcct caagttttct agagttgcgg ccgacagcga agtcgaagtg
     1741 gcgtgatgtc caatgtcgtt gcgcgggaga agaggcacgg ccgagctggc cagtcgaggg
     1801 ctattgacgt gcaaggacgc gaacgttcga gtcggtccgc gttcctgtcc cgagcggccc
     1861 tcatggccgg ttggctcgcg gcgagcggct gcttggggcc agcggccgtt cgcttcaccc
     1921 gcatgcaata caacgaggcg gtacgttcta caaacgacga gcagctcttg acgaacctgg
     1981 tgcgactccg ctacgccgac acgcccgtgt tcgtcgacct gcccaatatc accagccagt
     2041 tcgaactcgc ggcggggggg agtgacccgg gcccgagtgg cagccagacc aatttcggcg
     2101 tcgggggggc gtgggggcgc gattcgccga cactgagtta tcaccctcgc cagggacggg
     2161 agatcgccaa ggcgctgctg gacccgttgt cgcccaactt gtacagcgtg gtcaacgccg
     2221 gtgcgaggct cgaccagctc atgtggatga cgcttaacga catcaacgac gtgcagaacg
     2281 cggcccgggc cgcgaccctg gtcccgcgcg tccccgacga caatacgctc ttcctccgcg
     2341 gcatccaact gctcgcggaa atcgacgatc gcggcggtgc ggagatcggg ttctcgccga
     2401 tcgagaacaa aaagtccacc tccgacccgg tcctcgcggg ccacgtgcag gggcgtgacg
     2461 tgttggacgc ggcccggggc ggctatgtct accgcgacac gggggacggc cggatggctc
     2521 tgtacaagcg ggaaagcgtg ttgaccctga agatccgctc cccattcact cactcgcctg
     2581 agatggagga gatggcgcgg atctttaacc tgaccgcggg tctcccccgc tacaagatcg
     2641 agtctgaatt ggagcccggc gccgagagca ccccccccag tgcgctccca gtgggcgaca
     2701 cgatctacct caatttgcgg tcgattctcc agatcatgac cttcctgtcc aagggggtgt
     2761 gcatccccga tgaacacata cggaacggcg tggcgccgat gaccccggat gccgacggcc
     2821 gtccgtttga ctggacacga atcaccgccg gcaacttcgt cgtcgacgta cagaagcacc
     2881 ggccgcgcga cgccgaagtg gccgtgccgt accggggtta ctggttctac attcgccgcg
     2941 gggacgtgaa ctctcgatcc gtgctggccg tcctggagat cctcttttca ctccaggagt
     3001 cggacgagaa atcgaccggc ccagtgctca ccctgcccgc cggccggtga gccggtctac
     3061 ccgcgaggct ggccccggcg agcgataaag ccgggcgtgt gatctgtaca tccccccacg
     3121 ccgcggcgtg gccgtcggca acgggagtcg agatcggtca ccatgctcac tcggggtcca
     3181 cggtaccatc gctcccggcg tgttgaggag cgaacgaatt catgtaggag tcatgtcccg
     3241 cgtgaccgac gagcatggcc atcccattgc ggtccggatg cccatccggc ttgtgacgga
     3301 cctcgggctc tttcgtgccg ccaaaccact ggaccacata gtagaagacc ggcgtgagga
     3361 agatcccgaa gagggtcaca ccgagcatgc cggcaaatac cgtggtcccc agcgagcggc
     3421 gcatctcggc accggccccc tcggcgatga cgaggggcac gacgccgaag atgaaggcga
     3481 aggacgtcat gaggatcggc cgaagtcgca agcggcaggc ctgcagcgtg gcctctcggc
     3541 gggacgcccc ggcctcttgg ctctgtttgg cgaactcgac gatcaagatg
//
LOCUS       DATAQL010000785         7425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_692080, whole genome shotgun sequence.
ACCESSION   DATAQL010000785 DATAQL010000000
VERSION     DATAQL010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692080"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VHS97_13700"
     CDS             <1..212
                     /locus_tag="VHS97_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449309.1"
                     /translation="NAIAIGYFASKDSRDESDAMKPGPDAGRIVMQKLFIIRRRRGHG
                     QKERFVQEYKFSLFIALESALCWNT"
     gene            364..1290
                     /locus_tag="VHS97_13705"
     CDS             364..1290
                     /locus_tag="VHS97_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3449310.1"
                     /translation="MIRIRDLRVDYDNVCAVRDLSLEVAPGEVCGLIGPNGAGKTTTM
                     RAMIGLIEPTYGEINVMGVDVRENPQDVCRTVGFMPDFPPVYEDLLVWEFLDLFAASY
                     GIPRGKRPAEVGRHLEMVGLAQKRNALVVELSRGMRQRLMLAKTLIPEPRALLLDEPA
                     SGVDPQGRIDLKNILRRLAGEGKTVLISSHILAEMNEFCTSVAIMERGRLVVSGRIED
                     VNRRVMGDTLLSVEVLGESSLFLSVVGADSRASPVERKNGAYEFRFRGDALAASELLA
                     TLINQGVRVASFERRKDNLEELFLKVGSGGVS"
     gene            1290..2876
                     /locus_tag="VHS97_13710"
     CDS             1290..2876
                     /locus_tag="VHS97_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449311.1"
                     /translation="MNWERLYDWLDNPILVKHIRSRLRKQPLAAASVVVFVLCICIAW
                     AGMQLQSLASGGTFGLLLGLQSVILVVMGATQIGSAVGAARASGILDFHRVSPLTPTE
                     LTLGFFFGAPIREYVLFACTLPFSLFCLAFGTPSVHGFVQLMVLLIACAWLFHGLALL
                     NALLAKARANPRGVVGLIIFVVLFGGNLIFGMRRSAALVDMDLRLTFYGISLPWLAVV
                     LLHLAVVLYFIYLAARRKMGSERIHPLSKPQSIAALASLALLLVGTIWRREEFVTLEI
                     AALYLLVITAILLLMMVTPNLPEYDKGLWRARRMGLAHLPWWDDLSLNRVFLVIACAI
                     VLATATFAAGGAAESANSGFAASRDGGSFPLAIAAGVLVVAYFGLAHQYFALRFGGRA
                     RIYFSLFLFLAWVVPLLAGSILLMASMPIDAEPESQIVFSLSPVAGIGLTAMPGDARG
                     YRLAIQAAAITPALLFVFVFNTLLVAARRQIYKAFLRNAAAEIPISPAPTGERDGKVA
                     IAPAASSGFQAVPGMTGGLD"
     gene            2930..4105
                     /locus_tag="VHS97_13715"
     CDS             2930..4105
                     /locus_tag="VHS97_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449312.1"
                     /translation="MNLYHSVRQKTLVVVLATASLAAILPRDAIGADLRSSPNAQRRL
                     YVAEPGIRDYLEFGGHGLLVFDIDNGHRFVKRIATAGLDDKGKPINVKGVCASAATKK
                     IFISTIKQLMCLDLVTEKLLWEKSYEQGCDRMAMTPDGQYLFVPSFEGPLWYVVRAGD
                     GEIVERIRPDSGSHNTLVSLDGKEAYLAGLKSPLLTVVDTQTNRPVRAVGPFSASIRP
                     FTVNGRQTLCYVNVNELLGFEIGDMVSGRKLDRVVVKGFEKGPTKRHGCPSHGIALTP
                     NEKELWLADAFNSRIHVFDATVMPPKQVASIAVREQPGWITFSVDGQYAYPSTGDVIE
                     TRSRKIIASLTDELGRQVHSEKMVEVDFEGNQPVQAGDQFGVGRVSASGATSGATRP"
     gene            complement(4117..5070)
                     /locus_tag="VHS97_13720"
     CDS             complement(4117..5070)
                     /locus_tag="VHS97_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3449313.1"
                     /translation="MASATSARSAEPAATNVAAIQAKHDRAFIHELAEYLRQNPKADD
                     RDQAYAALFNKAIEHDWFSEAEELGRQYVKSDPDGPVKALAQIILTMARAQAGQFDQA
                     LNRFKELMQGLGQNEQEEFAASFADDLATTAIAAGEFATAREVYTQLLNRFKDSPNLR
                     QKVQNDLKRLDKVGKLAPSFAAEDIKGKTVRLDAYRGKYVLVDFWATWCAPCIGELPR
                     LQTAYRVHRANGFEIIGVSLDESKTAVVDFAKARNIPWPLVHNASGSADLVEAFGVSS
                     IPATYLIDPEGNIIRLDLRGKALDETLSRMIKPPATAVRQR"
     gene            complement(5177..6640)
                     /locus_tag="VHS97_13725"
     CDS             complement(5177..6640)
                     /locus_tag="VHS97_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3449314.1"
                     /translation="MVKQQHWIRQNLSPTNRSPRSGTPTAWIGIDPGGDLLAAPHSPV
                     EPAREQKPAGRILFINQYYWPDLASTAQHLTDLALALSAQGYECHVLASQSRYKPGEP
                     TPPAYEVHEGVHIHRVPATSLGRRGTWARMTDYLSFYAGALVTAMLLPRYDAVVTLTT
                     PPVIGLVGTVLKRFKKTRHVYWSMDLHPDASLALGRMSPKSLLGRSMSWLSSFVYRHA
                     DQVVVLGPYMADRIALKGVPSERIATIPVWSQRDEIYPTPRDANPIRKALGLEGAFVA
                     MYSGNLGLAHTFDEFIEAARLLRDRPNIVFLFVGGGPRLEEVKAARDREGLENIRLLD
                     YVPRAQLQASLAMADVHLISMRPEMTGIVVPGKLYGAMAAGRPAVFVGPEHCEPADTI
                     RDAGCGITIIPGDVDGLVAALLNLESSPSLARRMGEKGRSAFLTTYEHKLCCSQWSKL
                     IGESLARPETTRLRPTAPLQSRHTNHGAATPLLTASR"
     gene            complement(6634..>7425)
                     /locus_tag="VHS97_13730"
     CDS             complement(6634..>7425)
                     /locus_tag="VHS97_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3449315.1"
                     /translation="PCDVLFHTAAYYGGIGINQSEPAKLYYTNLVMGANLMEAARLSQ
                     VAKVVNIGTACSYPGYLEGHLKEQDLWSGPCHASVVNYGLTKKMLAVQGEAYKKQYGL
                     DSIHLILTNLYGPGDSYNPERSHVVAALVRKWVEADIAKAPSIEVWGTGKPIREFIYV
                     EDCADAIVLAAEKYNDVTLPLNIGTGIGTSIRELVETINSVTGFRGKIAWNADKPDGA
                     MLKVLDVARMKKALDGWAPPTDLKAGLAKTIAWYRANKTQADAKW"
BASE COUNT         1309 a         2141 c         2385 g         1590 t
ORIGIN      
        1 gcaacgcgat cgcgatcggg tacttcgcca gcaaggattc gagggacgaa tctgatgcca
       61 tgaaaccggg tcctgacgcg gggcgaatcg tgatgcagaa gttgttcatc atacgtcgac
      121 gccgcggcca tggccagaaa gagcggtttg tgcaagagta caagttttcc ttattcatcg
      181 cgctggaatc ggcgttatgc tggaacacat gaatctatcg tggccaggcg tcgacgcctg
      241 gccacgagag atcatcatca aacctagctg ccaccgtgaa ccgggaaggg agattgccat
      301 gccgaggttt attctcaccg agattgacga gcagacgcac cgacgggtgt tgtgtgatgc
      361 cttatgattc gcatccgcga tcttcgggtc gactacgaca acgtctgtgc cgttcgcgat
      421 ctttcgctcg aggttgcacc cggagaagtt tgcgggctga tcgggcccaa cggcgcgggc
      481 aaaacgacca cgatgcgggc gatgatcggc ttgatcgagc cgacctacgg cgagatcaac
      541 gtcatgggag tcgacgtgcg cgagaatcct caggacgtct gccgcacggt cggcttcatg
      601 cccgattttc cccctgtcta tgaagacctc ctcgtatggg agttcctcga cctgttcgcc
      661 gccagttacg gcattccccg cggcaagcgg ccggctgagg tggggcggca tctcgagatg
      721 gttggcttgg cccagaagcg caacgctctg gtcgtggagc tatcgcgggg catgcgccag
      781 cggctgatgc tggccaagac cttgatcccc gagccccgcg cgctcttgct cgatgagccg
      841 gccagcggcg tcgacccgca gggccgcatc gatctgaaaa acattctccg caggctggcc
      901 ggcgagggga agaccgtgct catttcgtcg catatcttgg cggagatgaa cgaattctgc
      961 acttcggtcg cgatcatgga gcgcgggcgg ctggtcgtca gcggccgaat cgaggacgtc
     1021 aatcggcggg tgatgggaga cacgctcttg tcggtggaag tgctggggga gtcatcgctc
     1081 tttctgtccg tcgtgggggc cgattcgcgg gcgagcccgg tcgagcggaa aaacggggcc
     1141 tatgagtttc gatttcgggg cgacgccctc gcggccagtg agctgctcgc gacgctaatc
     1201 aatcagggcg ttcgagtcgc ttcgtttgag cgccggaaag acaacctgga agagctcttc
     1261 ctcaaggtgg gttcgggagg ggttagttga tgaactggga acgtctttat gactggctcg
     1321 acaatccgat cctggtcaaa cacattcgct cgcgtctgcg caagcagccg ctggccgcgg
     1381 ccagcgtggt ggttttcgtg ctttgcattt gcatcgcgtg ggcggggatg cagctccagt
     1441 cgcttgccag cggggggact tttggtctgt tgctcggttt gcagagcgtc atcctggtgg
     1501 tcatgggggc gacgcagatc ggatcggctg ttggtgcagc gcgggcgtcc gggattctcg
     1561 acttccatcg cgtttcgccc ttgacgccga ccgagctcac actgggtttc ttcttcggcg
     1621 cgccgatccg cgaatacgtc ctcttcgcct gcacactgcc attttcgctg ttctgcctgg
     1681 ctttcggtac gccgagtgtg cacgggtttg tgcagctcat ggttctcttg atcgcgtgtg
     1741 catggctttt tcacggtctg gcgctgttga acgcactatt ggcgaaggcg agggccaatc
     1801 cgcgcggggt ggtcggcctg atcatcttcg tcgtattgtt cggtggcaat ctcatctttg
     1861 gcatgcgccg gtcagccgca ctggtcgata tggatctacg gctgacgttt tacggcatct
     1921 cgctgccgtg gctggcggtc gtgctcttgc atttggcggt cgtgctctac tttatctatc
     1981 tggcggcgcg gcggaagatg ggctcggagc gaatccatcc cctttccaag ccgcaatcga
     2041 tcgccgcgct ggcgagcctg gcgctgctcc tcgtcggaac aatctggcgg cgcgaggaat
     2101 tcgtgaccct cgagattgcg gcgctctacc tcctggtgat cacggcgatt ctcttgctca
     2161 tgatggtcac tcccaacctg ccagaatatg acaagggact gtggagagcg cggcggatgg
     2221 gcttggccca tttaccgtgg tgggatgacc tgtcgctcaa ccgggtattc ctggtgatcg
     2281 cgtgcgcgat cgtgctggcg acagcgacct tcgctgccgg aggggcggcc gagtcggcga
     2341 atagcggatt tgccgcgagt cgtgatggcg gctcatttcc attggcgatc gcggcgggcg
     2401 tgctggtcgt cgcgtatttc ggcctggcac accagtattt cgcgcttcgg ttcggcgggc
     2461 gggcccggat ttacttctca ctttttctgt tcctggcctg ggtggtgccg ctgttggcag
     2521 gctcgatctt gctgatggcg tcgatgccga tcgatgccga gcctgagagc cagattgtct
     2581 tcagcttgag cccagtcgcc ggtatcggcc tgaccgcgat gcccggtgac gcgcgggggt
     2641 accggctggc gattcaggct gcggcgatca cgccggcatt gctttttgtt tttgtgttca
     2701 acacactctt ggtcgcagcc cgccggcaga tctacaaggc cttcttgagg aatgcggcgg
     2761 ccgagattcc gatttccccc gcgccgacag gggagcggga tggtaaggtt gccattgcgc
     2821 cagctgcgag ttcgggattt caggctgtgc cgggcatgac cggcggtctc gactgaagtc
     2881 gaaagcgcgc ggtgcgtttt ccattaacag attgcagggg caaacaacca tgaatcttta
     2941 ccattcagtg cgccaaaaga cgctcgtcgt ggtccttgcg actgcgtccc tggccgcaat
     3001 cctgccgcgc gatgctatag gtgccgatct tcgctcatcc ccgaacgcgc agcggcggct
     3061 ctatgtcgcc gaacccggga ttcgcgacta tctggaattc ggcgggcacg gacttttggt
     3121 tttcgacatc gataatggcc accggttcgt caagcggatc gccaccgccg gcctggatga
     3181 caagggaaag ccgatcaacg tcaagggggt gtgcgcgagt gccgccacca agaagatttt
     3241 catcagtacg atcaagcagc tcatgtgtct ggacctggtc acggaaaagc tgctatggga
     3301 aaagagctac gaacaggggt gcgaccggat ggcgatgacg cccgacggcc aatatctatt
     3361 cgtgccatct tttgaagggc cgctttggta tgtggtgcgc gccggcgacg gagagatcgt
     3421 ggagcggatc agacccgact ccgggtcgca caacacgctc gtcagcctcg acggaaagga
     3481 agcctacctg gcggggctca agtcgccgct gctgacagtt gttgataccc agacaaatag
     3541 gccggtgcgc gccgtcggtc cctttagcgc aagcattcgg ccatttactg tcaatggccg
     3601 gcagaccctc tgttacgtga atgtgaatga gcttttgggc ttcgagattg gcgacatggt
     3661 ttcgggaaga aagctcgatc gcgtcgttgt gaagggtttc gagaaagggc cgaccaagcg
     3721 gcacggctgt cccagccatg ggatcgcgct gacgcccaac gagaaagagc tgtggcttgc
     3781 cgatgcgttc aacagccgca tccatgtttt cgacgcgacc gtcatgcctc ccaaacaggt
     3841 ggcgtcgatc gccgtgcgcg agcagccagg gtggatcact ttcagcgttg acggccaata
     3901 cgcctatccg tcgacaggag acgtgatcga gacccgctcg cgcaagatca tcgcttcgct
     3961 tacggatgag ctgggccgcc aagtgcacag cgaaaagatg gtcgaagtcg acttcgaggg
     4021 caatcagccg gtacaagccg gtgatcaatt cggagtgggc cgggtaagcg ccagtggcgc
     4081 cacttcgggc gccacccggc catgactcgc aaaatgtcag cgctgtcgca ccgcggttgc
     4141 cggcggcttg atcatccggc tcaatgtttc gtcgagtgcc ttgccgcgca ggtcgagcct
     4201 gatgatattg ccctccgggt cgatcagata ggttgctggg attgagctaa caccgaaggc
     4261 ctcgaccagg tccgcactac cgctggcgtt gtgcacaagc ggccaaggga tgttgcgcgc
     4321 cttggcgaag tcgacgacgg cggttttcga ctcgtcgagg ctcacgccaa tgatctcgaa
     4381 accgttagcg cgatggacgc gataggcggt ctgtaaccgt ggaagctcgc cgatgcacgg
     4441 agcgcaccag gtcgcccaga aatcgaccag gacatacttg ccccggtagg cgtcgagtcg
     4501 cacggtcttt cctttgatat cttccgccgc aaacgacgga gccagcttgc cgaccttgtc
     4561 cagccgtttc aggtcgtttt gcaccttctg ccgcaggttg ggactatctt tgaatcgatt
     4621 gagcaactgg gtgtaaacct cgcgcgcggt cgcgaactcg ccggccgcga tggccgtggt
     4681 ggcgagatca tcggcgaagc tggcggcgaa ctcttcctgc tcgttctgcc cgagcccctg
     4741 catgagctct ttgaatcgat tcaaggcttg gtcaaactgg cccgcctgag cgcgggccat
     4801 cgtcaagatg atctgggcga gcgccttgac gggcccgtcg ggatcgctct ttacgtactg
     4861 ccggcccagc tcttcggctt cgctgaacca atcgtgctcg atcgccttgt tgaaaagggc
     4921 cgcataggcc tggtcgcgat catcggcctt gggattctgc cgcaggtatt cggcgagctc
     4981 gtggatgaag gcccggtcgt gcttggcctg aatcgcagcg acgttggtcg ccgcgggctc
     5041 ggccgatcgc gcacttgtcg cggacgccat cgctccgatc gcgacgaaac aatacaatct
     5101 cttggatctc atcattgacc ctctctttgc aaggaaaaac tgggtcatat ttcaccaacg
     5161 atcatgtggt gctagattac cgcgaggcgg ttagcaacgg agtcgcggca ccgtggtttg
     5221 tgtgccgtga ttgtaagggc gccgtcggtc ggagccgggt cgtttcgggt cgagcgagcg
     5281 attcgccaat tagcttcgac cattgactgc agcagagttt atgctcgtaa gtggtgagaa
     5341 acgccgaacg gcctttttcg cccatgcgcc gagcgagact cgggctggat tccaggttca
     5401 agagtgcggc tacgaggccg tcgacatcgc caggtatgat ggtaataccg cagccggcgt
     5461 cgcgtatcgt atcggccggc tcgcaatgct cgggcccgac aaagactgct ggccggcccg
     5521 cggccatcgc accgtagagt ttgcccggaa cgacgatccc ggtcatctcg ggtcgcatcg
     5581 agatcagatg gacgtcggcc atcgccagcg aggcttgcaa ctgggcacga ggaacgtagt
     5641 cgagcagccg gatattctcg agtccctcgc gatcgcgtgc ggctttcact tcttccagcc
     5701 ttggaccccc gcccacaaac agaaagacaa tgtttggtcg gtcgcgcagc agccgtgccg
     5761 cctcgatgaa ctcgtcgaac gtatgggcca ggccgaggtt tcccgagtac atcgccacga
     5821 aggctccctc gaggccgagc gccttgcgta tcgggttagc atcacgcggt gtcgggtaaa
     5881 tctcatcacg ctggctccag accgggatgg tcgcgatccg ctcggacggt acgcccttga
     5941 gcgcgatccg gtcggccatg tacggcccga gcacgaccac ctgatcggcg tgacgataga
     6001 cgaaggagga cagccacgac atcgatctgc ccagcaggct tttgggtgac attcgcccca
     6061 atgccaggct ggcgtcggga tgaaggtcca tgctccagta aacatggcgc gtttttttga
     6121 accgcttcaa gaccgtgccg acgaggccga tcacgggagg cgtcgtcagc gtgacgacgg
     6181 catcgtagcg cggcaagagc atagccgtga ccagagcgcc ggcgtagaaa ctcaggtaat
     6241 cggtcatccg cgcccacgtg ccgcggcggc ccagcgatgt cgctggtacg cggtggatgt
     6301 ggacgccttc gtgtacttcg taggcaggcg gcgtgggctc gcctggcttg tacctgctct
     6361 gcgaagccag tacgtggcac tcgtaaccct gggcgctcaa cgccagggcg aggtcggtca
     6421 agtgttgggc cgttgacgcg aggtcgggcc aataatactg gttgatgaac aggatccgtc
     6481 ccgcgggctt ttgctcgcgt gctggttcta ccggggagtg gggcgcggcg agaaggtcgc
     6541 cgcccgggtc gattccgatc caagcggtgg gcgtgccgga ccgcggcgat cggttcgtgg
     6601 gggacaggtt ttgtcgaatc cagtgctgtt gcttcaccat ttggcgtccg cctgggtctt
     6661 gttggcccgg taccaggcga ttgtcttggc cagcccggcc ttcaggtcgg tcggtggcgc
     6721 ccatccgtcc agggcttttt tcatcctggc gacgtcgagc actttcaaca tggccccgtc
     6781 gggcttgtcg gcgttccagg cgatcttgcc tctgaagccg gtgactgaat tgattgtttc
     6841 taccagctct cgaatcgagg taccgatgcc ggttccgata ttgagcggca gagtcacgtc
     6901 gttgtacttt tcggcggcga gcacgatcgc atctgcgcag tcctcaacat agatgaattc
     6961 gcggatcggc ttgccggtgc cccacacctc gatgctgggt gccttggcga tgtcggcctc
     7021 gacccatttg cgcacgagcg cagcgaccac gtgtgagcgc tcgggattgt acgagtcgcc
     7081 tggaccgtag aggttggtga gaatcagatg aatcgagtcg aggccgtact gcttcttgta
     7141 cgcctcgccc tgcacggcga gcatcttctt ggtcaagcca tagttgacca cgctggcgtg
     7201 gcacgggccc gaccagagat cctgttcctt caagtgtcct tcgagataac cgggatacga
     7261 gcaggccgtg ccgatgttga cgaccttggc gacctgagaa agtcgcgcgg cctccatcag
     7321 gttagcgccc atgacaagat tggtgtagta gagcttggcc ggctcgctct gattgatgcc
     7381 gatgccgccg taatacgcgg ccgtgtgaaa caagacatcg cacgg
//
LOCUS       DATAQL010000786         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_79848, whole genome shotgun sequence.
ACCESSION   DATAQL010000786 DATAQL010000000
VERSION     DATAQL010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79848"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VHS97_13735"
     CDS             <1..188
                     /locus_tag="VHS97_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HEX3449316.1"
                     /translation="VMPTGGVDLSTAKSFLDAGACCLGIGSSLVDPKAVASGDFTRIR
                     DLATQYISIVRRSRGLA"
     gene            541..2340
                     /locus_tag="VHS97_13740"
     CDS             541..2340
                     /locus_tag="VHS97_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449317.1"
                     /translation="MGPKRRGKRLALLRPSLTALALRLGAVIASAAAIAPLAAWAAPE
                     KTQASPVPKVYHKGRNFRIPFNLHDAGKDRVKELHLLVSEDLGYHWDSKSKTFPDHPT
                     FTFRSAHDGEYWFAVQTLTVDGKVAPALDATVEPNMKVVVDTFPPTLLLEPEERRGSA
                     ASVRWEVKDENLDLKTLVLEYQAEGAGSWRRVPIRRPKLIGAQQWDAGTAEALKVRAS
                     VADKAGNVSDAMIELPEGTANLPDLASSDPGDDVPPAVSQVSNDLNPDISAGPSFTPV
                     NQAPAQNRKVQSGSGRAGRASAGRSSASVARGARPASPDWDRESGSPAAARPTLPNDD
                     AARTDLFASAGGPAPFAGGGSAGDSARPRPPAFGQSASDEGNGGTLLVASPMFKLQYA
                     VEDAGPNGPATVELWITKDGGRTWIRRADDQDRISPIEVDLGGEGTYGLYLVARSASG
                     LGDQPPAPGDPPQSWVEVDTTAPAVQLQPPQVGTGVNSGKVAIAWRASDLHIPSKAVS
                     ISWRPDQPGADWQTIADAQENAGQYIWTVPPTAPQRFHLKVEATDSVGHRGSADTTEM
                     GPVMVDRSRPRSRIIGLDPNARAGIGPSARPLR"
     gene            complement(2368..>2771)
                     /locus_tag="VHS97_13745"
     CDS             complement(2368..>2771)
                     /locus_tag="VHS97_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX3449318.1"
                     /translation="RIHGKPATQVIGEPSWFTEAFIPTVAKRGSAVIKARGASSAASA
                     ANAAIDSVRSLDLPTAHGDWFSAAVVSDGSYGIPAGLIYSFPLVSKGSAAWSIVPNLP
                     IDAEARARLDASAAELVSEREAVKELLGPAL"
BASE COUNT          544 a          868 c          857 g          502 t
ORIGIN      
        1 gtgtcatgcc caccggtgga gtcgatctgt cgaccgccaa gagcttcctc gacgcgggtg
       61 cctgctgcct gggtatcggc agctcgttgg tcgatccgaa ggcagtcgca agtggagatt
      121 tcacacgaat tcgggacctg gcgacccagt acatttcaat cgttcgccga tcgagaggac
      181 tggcttagca aacaactaac cattactcgc tctgggtcag ctcaaataaa cttcacatac
      241 ctcccggtca tccatgggcc cggaatttta cccagagttg cacaagttcc tactatccgt
      301 ggatcaacca gcgcgattga ttcgatccgg acatccggcc gattcacttc aagccgaccc
      361 ggagccaagg acgataaact cgccatgaga cgagggccgc gctcttggtc cgacctcgta
      421 gtcgcggcct ggagttgtgg tcatatgcgc agtgtattgg tatttaggta aaggttgagc
      481 tccagaggaa ctaccgccac aagagcaaga tgcggccgaa tttgcgggga gtcgatacgc
      541 gtggggccaa aaaggcgagg aaagcgcctg gcgctcttga ggccgagctt gacggctctg
      601 gccctgagac tcggcgcggt tatcgcatcg gccgcggcta tcgcgcctct ggctgcctgg
      661 gcggcacccg aaaagacgca agcgtccccg gtgccgaagg tctaccacaa aggccgcaac
      721 ttccgaatac cattcaatct ccatgacgcg ggcaaggacc gtgtcaagga gttgcatctg
      781 ttggtctccg aagatttggg ttaccactgg gattcgaaaa gcaagacatt tcccgaccat
      841 cctacgttta catttcgctc ggcgcatgat ggagaatact ggtttgctgt acagacattg
      901 acagtcgacg gcaaggtcgc gccggcactc gacgcgactg tcgaaccgaa tatgaaggtt
      961 gtggtcgaca cgtttcctcc gacgctactt ctcgagcccg aagagcggcg aggcagcgca
     1021 gcctccgtac gttgggaggt caaggacgag aatctcgact tgaagacatt ggtactggaa
     1081 tatcaggctg agggagccgg atcgtggcga cgcgtgccca tccggcggcc gaagctgatc
     1141 ggcgcccagc aatgggatgc cggcacggcg gaggcgctca aggtgagggc ctcggttgct
     1201 gacaaggctg gcaacgtgag cgacgccatg atcgagcttc ctgaggggac cgccaacctg
     1261 ccggatcttg cgtcaagtga ccctggcgac gatgtgccgc ccgcagtctc gcaggtctcc
     1321 aacgacttga atcctgatat ctctgccggc cccagcttca cgcccgtcaa ccaagcccct
     1381 gctcagaatc ggaaagtgca atcgggctcg gggcgcgcgg gtcgagcatc ggctgggcgt
     1441 tcgagcgcgt ctgtggcgcg aggtgcgcgg ccggctagtc cggactggga tcgtgaatcc
     1501 ggttcacccg cagccgcgcg gccgactctt cccaacgatg acgcagcgcg tactgacctg
     1561 ttcgcctcag cgggaggccc cgctccattt gctggtggtg ggtctgcagg cgactcggcc
     1621 cggccccgtc caccagcctt tggccagtcc gccagcgacg agggaaatgg tggcacgctc
     1681 ttggtggcca gtcccatgtt caagctccaa tatgccgtcg aggatgcggg tccgaacggc
     1741 cccgccacag ttgagctatg gattaccaag gacggcggcc gcacctggat tcgccgcgct
     1801 gacgaccagg accgaatctc acccatcgaa gtcgacctcg gtggcgaggg gacatacggt
     1861 ctttacctgg ttgctcggtc ggcctcaggg ctgggcgatc agccccccgc acccggcgat
     1921 ccccctcaat catgggtcga agttgatacc acggcacccg ccgtccagct tcagccgccg
     1981 caagtcggca cgggcgtcaa ctccggcaag gtggcgatcg cgtggcgggc gagcgacctt
     2041 cacatcccct ccaaagcggt ttcgatctcg tggcgtccgg accaaccggg cgccgactgg
     2101 cagacgatcg cggatgctca ggaaaacgcc ggccaataca tctggactgt tcctccaact
     2161 gccccgcaac ggttccatct caaggtcgaa gctaccgact ccgtcgggca tcgcggctcg
     2221 gcggacacga ccgagatggg ccctgtcatg gtcgatcgca gcaggccccg cagccggatc
     2281 atcggactcg atcccaacgc ccgcgccggt atcgggccct cagcacggcc gctgcgttag
     2341 ctaaacgggg cggggatcaa acaaacgcta gagcgcaggg ccgagcagct ccttgacggc
     2401 ttcgcgctcc gagaccagct cggcagccga agcatcaagc ctcgctcggg cttctgcatc
     2461 gattggcaaa ttcggaacga tcgaccatgc tgcgcttccc tttgagacca gtggaaagct
     2521 gtagatcaag cccgcgggga tgccgtagct tccatcggat acaacggcgg cgctgaacca
     2581 gtcgccgtgt gcggtaggca ggtcgagcga ccgaacgctg tcgatcgctg cattggccgc
     2641 cgaagctgcc gatgaagccc cccgggcctt gatgaccgcg gagccgcgct tggcaaccgt
     2701 cggaatgaat gcctcggtga accaagacgg ttcgccaatc acctgggtcg ccggcttgcc
     2761 gtggatcctg g
//
LOCUS       DATAQL010000787         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4507927, whole genome shotgun sequence.
ACCESSION   DATAQL010000787 DATAQL010000000
VERSION     DATAQL010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4507927"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1094)
                     /locus_tag="VHS97_13750"
     CDS             complement(21..1094)
                     /locus_tag="VHS97_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449319.1"
                     /translation="MSSTPTPGKPPRRPVRSGELERESEVEGPPVFVPAPPAPDRPTG
                     KWRWFLLGLLGILAIGAVGVMVAWPRLQPKKLDAVERVADAYLKALSGDDPDVARKYS
                     TVEEPPAIRSVRNVKRQRDRDRMLKGSFAPLGKFHSGIESEFNFDASIGRFTPKNAMG
                     AAAETLDALHAAKEDAEKSGLYKKMASGDPDDLFDAAEQYGKVFTKLAEGALAPKRIL
                     PTYQMLVESAKPPLPKDANALALEVAGSMKDWSGLLKRPFQTLKADGPFIYEKAEVTA
                     LANDRLASLGDPPSRLRLELVWFRLEGIDTGWKVVSARRVLPGDDKQDAKPAATPSSP
                     ASKSEAPSPGDVRRSPGDTPTPQ"
     gene            complement(1096..1515)
                     /locus_tag="VHS97_13755"
     CDS             complement(1096..1515)
                     /locus_tag="VHS97_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449320.1"
                     /translation="MNRAPNERIRYDGLRKGRRTLGLLAPLVQVAFLALGFGIFLDQS
                     RSLLSDAQYTWGERQVMGIIALLALGGCGFAGWVVGRLIRVAAELLDVLADDAEANGR
                     TNELIERHVVPALGRIALALERIETSGDRITTNRERP"
BASE COUNT          345 a          597 c          531 g          329 t
ORIGIN      
        1 gcggaatctc cgcccgaggg tcactgcggt gtcggcgtat cgccaggcga tcgccggacg
       61 tcccccggcg agggcgcttc agatttcgac gccggggacg agggcgtcgc ggcgggcttg
      121 gcgtcctgct tgtcgtctcc cgggagaaca cgtcgggccg acacgacctt ccagcctgtg
      181 tcaatcccct cgaggcggaa ccataccagc tcgagccgca ggcggctcgg cggatcgccg
      241 agcgaggcca ggcgatcgtt cgccaaggcg gtcacctcgg ccttttcgta gatgaacggt
      301 ccatccgctt tcaacgtctg aaacggccgc ttgagcaagc cgctccagtc cttcatcgat
      361 ccggcaacct cgagtgccag cgcattcgcg tctttgggca acggcggctt ggccgactcg
      421 accagcatct gatatgtcgg caagatccgc ttgggcgcga gcgctccttc tgccagcttg
      481 gtgaagacct tgccatactg ctcggccgcg tcgaaaagat cgtcggggtc gccactggcc
      541 atcttcttgt aaaggcccga cttctcggca tcctccttgg ccgcgtgcaa ggcgtcgagc
      601 gtctccgcgg ccgcccccat cgcgttcttg ggagtgaatc ggccgatcga cgcatcaaaa
      661 ttgaattcgg actcgatgcc actgtggaat ttacccagcg gcgcaaacga acctttcaac
      721 atccgatcgc ggtcgcgctg gcgcttgaca tttcgcacag agcgaatcgc cggtggctct
      781 tcgacagtgc tgtacttgcg cgccacgtcc ggatcgtcgc cgctcagggc ttttaaatat
      841 gcgtccgcga cccgctcgac tgcgtccagt ttttttggtt gaagccgcgg ccacgcgacc
      901 atcacgccga ccgcgccaat cgccaagatt ccgagcaggc ccagcaaaaa ccaccgccat
      961 tttcccgtcg gacgatccgg tgcgggaggt gcgggcacga agactggcgg accttcaact
     1021 tcactttcac gctctagctc gccggaacgc acgggccgcc gcgggggttt gccgggagtg
     1081 ggtgtcgagc tcatgtcagg gacgctcccg attcgtcgtg attcgatcgc cagaagtttc
     1141 gatccgttcc aaagccaagg cgatccgacc gagagccggt acgacgtgcc gctcgatcaa
     1201 ctcgttcgtg cgcccattag cctcggcgtc gtcagccagc acatccaaaa gctccgccgc
     1261 aacacgtatc agacgcccca cgacccaccc cgcgaaccca caaccaccca gggccaatag
     1321 cgcgattatc cccatcacct ggcgctcgcc ccaggtgtat tgggcgtcag acaagagcga
     1381 tcgcgactga tcgagaaaaa tgccaaagcc caaggcgaga aatgcgactt gcaccaaggg
     1441 agccaaaagg ccgagtgtgc gccggccctt gcgcagtccg tcgtaacgta tccgttcgtt
     1501 cggggctcga ttcatggctc acgctagtat actggccgcg gtgcgaggca ggagtgtggc
     1561 gaagggaggg cgaggctcca gccgagctgg cgttggcact cggctcgccc tcccgggatg
     1621 tctcaagcgg cccgcacgca gtataaccgc cacagttcgt aatcgacaat tgtgtgatta
     1681 tagtcgctcg agcacgtaaa aacctcttcg ttcgcccgac gaatcgtccc tgacgaaatg
     1741 gaccgcacga tagccggccg caaacgccgt ctgaaatgct cgaccaacaa gagcacgcca
     1801 gc
//
LOCUS       DATAQL010000788         4697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5118808, whole genome shotgun sequence.
ACCESSION   DATAQL010000788 DATAQL010000000
VERSION     DATAQL010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5118808"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            645..2630
                     /locus_tag="VHS97_13760"
     CDS             645..2630
                     /locus_tag="VHS97_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449321.1"
                     /translation="MPTSTNQPTQSTSNRTARISARHGSRDAKTGRLVVLGSLLLILG
                     ITARGQEPDTRSDSSRSQPPSTTAPTVSRNSVVGPLDADAAALRAETLERLKVFEPTV
                     TTDTVSSTEPTRPNTAAGPISPAAATPSTHSFGAAAVTSPSSDPTGSKSLPDLLRDRL
                     RWLKEYESASVALQKAVHPEPSPAQEAAEAKEELARLKEILAQAAHSLESLLPPLFRG
                     PTAKVSTAIASEMKDALESTSNELKDWTTKLETFRSEIAKWDSLQKSRHIERDRLFQQ
                     VTALTAKSQEYDAAVADAPTALARRLAHERLVNFQWAARVETLRLQVIEAQLALEAKL
                     AEVRELKVEIYTAHVQIANSTLGPMQSRYKLVSEDQERDLSQAKANEENKAQLSADPL
                     ERFRARRTAELMALEILVNRSEQARTISPTPTYEDQKTLADHAEIDFANIKELLDDGK
                     VSRLDAVRLNNEFRRIGPERDRLLRNEMATVESQLQYYENSLTEVELELLQDSLHDRF
                     EHDLLREGLPQARWAEGETLVKDLERKQRLLLVRRRDALEKLAERASHTLQQVVRRLD
                     ILDSEYAFIRTNIFWVRDQEPIGLLTITQAAREFNHLVKGMLRLAQETVKPNLWGQPS
                     AEFLITALAVLALPVALVRLRRMLEALVKCDLPAPST"
     gene            2784..>4697
                     /locus_tag="VHS97_13765"
     CDS             2784..>4697
                     /locus_tag="VHS97_13765"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX3449322.1"
                     /translation="MQALRSLGLGLARASIWPLDLVLVAYVSRVAPWPRSLGILVSAV
                     MTGAAIAIFVHEVLRLLTWPSCWAERNLGVPGAVARQLGWDGRFVVIAAMVFLLPVYL
                     LNHELIAPEGKPITAPALGRLLVLGFELFAWGTCVRLVRGVSPLLGWLSIPLAPASDG
                     EALNAAAIAPAVPTTPVTAHEGQLAASARFYAALFWLGRRRRVVAGFVIAAIAVIIVL
                     DVRGYSFTARRVAAGGIQTAVAIALAVATYRVILQAIGRNAWRWALPNRSWAMALTSA
                     MALRAKARSRIAAAAPIFEPSVAHPDAADLEDDAQLADLTSGLKHLTAYALTALTVLT
                     IAWIWEVDMALVQFLLNQPLWAADAQTFVTVGDLAEASVVIVLGALSWRYMNTLFAVI
                     LFPRMTDDPGVRFAVVTLCRYTVLGIASLVALAAIHLDVAKIGVVLAALGVGLGFGLQ
                     EIVSNFVCGIILLLERPIRIGDVVTVAGTTGNVDRINIRATTIINSDNQSMIVPNRQF
                     ITGNLVNWTLKDKILRVQIKVSVAYGSDPERVVNLLLGIARADSDVMINPAPSAALEG
                     FGESSLQFALYTFVPEPGLSGGVRHRLCSEIQRRFTADGIVIPYPTHELHVNRVPEGQ
                     TRALETARADVIPP"
BASE COUNT          929 a         1461 c         1360 g          947 t
ORIGIN      
        1 tcatacgcgt accaaatttc gcgctttcaa taacatgctg ccgaccaatc tggtttgccg
       61 ctcaggcaac ccgtcacata gccatctgca aaacctgtca gccaaacaag ttcggtccag
      121 ccagcagtcg ccgctttacg attgccaaac cgaccgtctg gtcggccgtc gttgtgaggt
      181 ctacgtggac catgcaagca ctattcgacg atggtcacaa gcaaggtccg cgagaaacga
      241 gcagatgcaa atggtcgttc gccgggtcaa tcatggcaac ccattcgtac caaatgatag
      301 cgcattagta agtcgcgtca agagtgtcca agcccctttg gcccatccgc tgcccgcaaa
      361 gtgaattcgt cgtggactga tcccttcagc cacaacggaa atcgagtcgc tcgtttatcg
      421 gttcagttgc gtaatgttag tgaaaaactg tacttaggac ggcgaacggt cgcactctgg
      481 tgcttagtcg agcgtttcct ccatcaaccg aaacgagatt ctaggtgcgc cgagcctctg
      541 tgtcatttcc acttgccgca ttgggtgcat cgggatactt tacgccgctc gcgccgtacg
      601 cgagactgag ccgctcaatg tcttgccggg aggtagcccg ctcgatgcca acatcgacga
      661 atcagccaac ccaatcgacg tccaaccgga ccgctcggat ttcggctcga cacggcagcc
      721 gggatgcaaa aaccggtcgt ctcgtcgtgc ttggctcgct cttgttgatt ttggggatca
      781 ccgcgcgagg ccaagagccc gacacgcgca gcgactcctc ccgctcgcaa ccgccctcca
      841 cgacggctcc cacggtgtct cgaaactcgg tggtcggtcc ccttgatgcc gacgcggcgg
      901 cccttcgagc cgaaacgctt gaacggctca aggttttcga gcccactgtc acaaccgaca
      961 ctgtctcatc gaccgaaccg acgaggccca acacggctgc tgggcccatc tctccggcgg
     1021 ctgcaacgcc ttcaacacac tcatttggag cggctgctgt tacttccccc tcaagtgatc
     1081 ccaccgggag caaatcgctt ccggacttgt tgcgagatcg gctgcgctgg ctgaaagagt
     1141 atgagagcgc atcggtggct ttgcagaagg cagtacaccc cgaaccgagc ccggcacaag
     1201 aggcagccga ggccaaggag gaacttgcca gactcaagga gattctcgcg caagcggccc
     1261 actctctcga gtcgctctta cccccccttt ttcgcggacc aacggccaag gtctcaaccg
     1321 cgatcgcctc ggaaatgaaa gacgctttgg agtcgaccag caacgagctg aaggattgga
     1381 cgaccaagct ggaaacgttt cggagtgaaa ttgcgaaatg ggacagtctt cagaagtcgc
     1441 gtcatatcga gagagacagg ctcttccagc aggtgacggc gctgacggcc aagagccagg
     1501 agtatgacgc ggccgtcgcg gatgccccca ccgccctggc gcgtcggctg gcgcatgagc
     1561 gacttgtcaa cttccagtgg gccgcgcgtg tcgaaacgct tcgccttcag gttatcgagg
     1621 cgcaactcgc gctcgaggcc aagctggcgg aggtccgcga actgaaggtg gagatttaca
     1681 cggctcacgt ccagattgcg aatagtacgc tcgggcccat gcaatctcgg tataaacttg
     1741 tctcagagga ccaagagcgt gacctgtcgc aggccaaggc caacgaagaa aacaaggcac
     1801 agttgtctgc cgaccccctc gagcgcttcc gcgcccggcg cactgccgag ttgatggccc
     1861 tggagatact ggtcaataga agcgagcaag ccagaaccat cagcccgacg cctacctacg
     1921 aagaccaaaa gacgctcgcc gaccacgccg agatcgattt cgccaatatc aaggaattgc
     1981 tcgatgacgg caaagtcagc cggctggacg cagtccgctt gaacaacgag tttcgccgca
     2041 tcggtcccga gcgtgatcga ctcctccgaa acgaaatggc caccgtcgaa tcgcagcttc
     2101 agtattacga gaattccctc accgaggtcg agctcgaact gcttcaagac tcgctccacg
     2161 atcgcttcga gcacgatctc ttgcgcgagg gcctgccgca ggcgcgctgg gcggaagggg
     2221 aaaccctcgt caaggatctc gagcgtaaac aacggctgct tctggtgcgt cgccgagacg
     2281 ccctcgaaaa gctggccgag cgcgcatcgc acaccctgca acaggtcgtt cgccgactcg
     2341 atatccttga tagtgagtat gctttcattc gaacaaatat cttctgggtc cgcgaccagg
     2401 aaccaatcgg gcttttgacc attacccagg cggctcgcga gttcaatcat ctggtcaagg
     2461 gcatgctgcg actggcccag gaaacggtca agccgaatct gtgggggcag ccctccgccg
     2521 agtttctgat cacggcgctc gccgtgctgg ctctccccgt cgcgctcgtg cgtctgcggc
     2581 gaatgctcga agcgttggtc aagtgcgatc tgcccgctcc cagcacttga gcgccgctcg
     2641 aattccgcca tggcccgcac gccaggcaca cgagatccgg acgggatcta ttgaaatggt
     2701 gatgaaattt cattcacgtc gtacgtgaat attcattcgc aggatcgacc gagaaaaacg
     2761 cgagaagcaa ggacggatca aaaatgcaag ccttgcgatc attggggctg gggttggcca
     2821 gggcatcgat ctggccgctc gatctggtct tggttgcgta cgtgtcccgc gtcgctccct
     2881 ggccacgaag cctgggcatc ttggtctctg ccgtaatgac aggcgccgcg atcgcgatct
     2941 tcgtccacga agtattgcgc ttgctcacat ggccctcgtg ctgggcggag cgcaatctgg
     3001 gcgtgccggg ggcggtggcg cggcaactcg gctgggatgg ccgtttcgtg gtcattgcgg
     3061 cgatggtttt tctgttgcca gtctatttac tgaaccacga gttgatcgcg ccggagggta
     3121 agccgatcac ggcgcccgca ttgggccggc tgctcgtact tggttttgaa ctgttcgcat
     3181 ggggaacgtg cgtccggctg gttcgcggcg tctcaccgct cttgggttgg ctctcgattc
     3241 cgctcgcgcc ggcgtctgac ggtgaagcgc tgaacgcagc ggcgatagcc cctgcggtgc
     3301 ccacgacgcc cgtcaccgcc catgaaggcc aattggcggc atccgcgcga ttctacgcgg
     3361 cgctattctg gctcggccgt cggcgccgcg tggtcgctgg cttcgtgatc gccgccatcg
     3421 ccgtgatcat tgtcctcgat gtgcgcggct atagctttac tgcccggcgc gtggccgcgg
     3481 ggggcattca aaccgcggtc gcaatcgcct tggccgtcgc gacttatcga gtgatcttgc
     3541 aggccatcgg tcgcaatgca tggcggtggg ctcttcccaa tcggtcctgg gcgatggcgc
     3601 tcacgtcggc gatggccttg cgagcgaaag cgcgatcgcg tatcgccgcg gccgccccga
     3661 ttttcgagcc ctcggtcgcc cacccggatg ctgcggatct tgaagatgac gctcagcttg
     3721 ccgacctcac gtccggcctg aagcacttaa ccgcctacgc gttgacagcc ctgacagtgc
     3781 tgacgatcgc atggatctgg gaagtcgata tggctctggt ccagttcttg ctcaaccagc
     3841 ccttgtgggc agccgatgcg caaaccttcg tcaccgtggg cgacctggcc gaagcatccg
     3901 tcgtcattgt gctgggggcg ttatcgtggc gttacatgaa cacactcttt gccgtcatac
     3961 tttttccccg catgaccgat gatcctggtg tgcggttcgc cgtcgtcacc ctctgccgct
     4021 ataccgtact gggcatcgcg agcttggtcg cactcgcggc cattcacctc gacgtggcca
     4081 agatcggtgt cgttttggcg gccctgggcg tgggactggg gtttgggctt caggagatcg
     4141 tctccaactt cgtttgcggg atcattctgc tcttggagcg tccgatacgc atcggcgacg
     4201 tcgttacggt cgcgggaacc acgggcaatg tcgaccgcat caacattcgc gccacaacga
     4261 tcatcaactc ggacaaccag agcatgatcg ttcccaatcg ccagttcatc acgggcaacc
     4321 tggtcaactg gacgctcaag gacaagatct tgagggtgca aatcaaagtt agcgttgcct
     4381 acggatcaga ccccgagcgg gtcgtaaacc ttttgctcgg aatcgcgcgc gccgactcag
     4441 atgtaatgat caacccagcg ccatcggcgg cgctggaagg ctttggagaa tcatcgttac
     4501 agttcgccct atacacgttc gtccccgaac ccggcttatc gggcggcgtg cggcatcgac
     4561 tctgctccga gatccagcga cgctttaccg cagacggcat cgtcattccg tatccgacac
     4621 acgaactcca cgtgaatcga gtgcccgagg gccagacccg agcgctggaa accgcccgag
     4681 cagacgtgat cccgccc
//
LOCUS       DATAQL010000789         5458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2981946, whole genome shotgun sequence.
ACCESSION   DATAQL010000789 DATAQL010000000
VERSION     DATAQL010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2981946"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VHS97_13770"
     CDS             <1..1017
                     /locus_tag="VHS97_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449323.1"
                     /translation="AWAILPRRARQKVTLVTPGSLFLEKVFEAIPLIDLDVVKVPPDG
                     MAPAAGAAATGASASITIYDRKVADILPAGHVLVIEPEKSGPLWDLGETIHNPVVAKQ
                     DKDSPLMAHIRLDNVLMPEARKLALKLPGKVLAESASGDALYAVLDRPEPAQQGKVVL
                     LTVNLDKSDLPLQTAFPIMVTNLLNWFGGTKGELREALAAGSVAEVELPADKSAESHF
                     VLKSPGGTETPVVVPAGAAKVTVGPLDRTGVWRVLKRSAKGLVSAKKGKDAIDPRDVV
                     AELACNLADRRESDLRPAVEPADRRPGLAAGLAVRPIWYYLLASALMLTCWEWFLYQR
                     RWID"
     gene            1017..3692
                     /locus_tag="VHS97_13775"
     CDS             1017..3692
                     /locus_tag="VHS97_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3449324.1"
                     /translation="MMALLPIAFDFQLELTRPWWLLGLAALPILVYFFYRTLVDFARW
                     QRVLSLWLRGVIVVLLALALAGLSLVRPTRELFVVFAMDRSASIGEQGNRAIDDFLTK
                     SAAHAGSNRFAVLPFASEPGVVRTSDEALAALKNSKDAAPKKEPVAGASAAEPEDARR
                     LDRKGTDLAAALQVAAAAIPPFYVPRIVLLSDGNPTNGDVLKVAASLNGKVDVFTSTL
                     PVRDDPEVQLSAVAAPAQVLQGEPFKVEVLIDTNHDDSEGQLEVYRGDIKVAGQPVAL
                     KKGENRIVLKQTIDQGGLTPITARLKGYKDTLLDNNSEFGLVSAAGKPRVLLVDSDPD
                     QAKHLTWALEEQNMQVDVRPPRGVPDNLAELQNYDLLMLSNVPATALSLRQMEVARTY
                     VQDLGGGLIMLGGDQSFGLGGYYKTSLEEILPVRSDFEKEKEKPSLAMMLVIDKSGSM
                     GGEKIEMAKEAARAAVELMGPSDKVGVLAFEGENFWVSELHPCSDKGFVLNRIAALEA
                     GGGTDMAPAMEEAHETLRGAVAKLKHVIVLTDGISRPGDFEGIAQGMAADRITCSTVA
                     MGGDADQSLLEEIARLGNGRFYVADDPAQVPQIFAKETVTASKSAINEQPFAPTIVRS
                     TQVLSEIRLDEAPFLLGYVVTRPKPTAEVILATEAGDPLLAWWRYGLGMTAAFTSDAK
                     ARWAAEWLSWPQFGQFWAQVVRHSLRRAEAKGTLVQVERAGRKSVVSIDAIEPSGKFL
                     NQAATDLTLVDPKLATRKIEMAQVAPGRYQAEFDTTQPGSYQLMFSQTRDSQLLGRQT
                     RGLAVGYPDELRLRPANAELLKSIAGASGGRFDAKPESVFDVPDRSAPRAVPLWPYLA
                     TAAALLFVLDVALRRVDLTLLLPRRRQISLSTRAF"
     gene            3779..5275
                     /locus_tag="VHS97_13780"
     CDS             3779..5275
                     /locus_tag="VHS97_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449325.1"
                     /translation="MSERILEKLEPVRRRQQGLEVMRSTAAGLLASSLVGVGLGLLRW
                     QGAGVTGAGRWWLWAAAIAVAGPALGAIVGLARGRSALLAAAAVDRQYGLKDRALSAV
                     DFIRRGKSTSLHTLQVADALEHLAGLDPRQVAPFRMPAVLPYAVATMALALVLLLWPR
                     AGVVQAEPAAPLEAVLAAAEDAEDGLEELNEAAKKNDDIKLKELVQKLSETIQEMKLP
                     GVDTKEALAKLSEMQAAITAQQAQFNVGLVDAQMQSLGEAMASTQALEGAGQALQQGK
                     YDKAAEQLEKAEPKFDRKETKTLKEKLAQAAKAMEDGGLTELSTATTELAASLDDEGT
                     AQGACKKLGNLARSQGRRKRIADLLTLATVKLGECKGNCDKNGGAKLRLRKKSETPSS
                     KWGLAISGNTDGEKTKLDAARKREQIQGQMGEGDSETETTHMPEGRQTAARTYREQYQ
                     KYRRMTEAALNSEPIPLGHRQTIRRYFELIRPQNDEAEKADATVQPGK"
BASE COUNT         1025 a         1650 c         1804 g          979 t
ORIGIN      
        1 gcctgggcaa ttctccctcg ccgggctcgc cagaaggtga ccctggtgac ccccggcagc
       61 ttgttcctgg agaaagtttt tgaggcgatt ccgctcatcg atctcgacgt cgtcaaggtt
      121 ccgcccgacg gcatggcccc cgcggctggt gccgcggcaa cgggtgcttc cgcctcaatc
      181 acgatctatg atcgcaaggt cgctgatatt ttgccggccg gccatgtgct ggtgatcgag
      241 cctgaaaaat ccggtccgct ctgggatctc ggcgagacga ttcacaatcc ggtcgtcgcc
      301 aagcaagaca aagactcgcc gcttatggcc catatccggc tagacaacgt cctgatgccc
      361 gaggcgcgca agctcgcctt gaagttgccc gggaaagtac tggccgagtc ggcctcgggc
      421 gacgcgcttt acgcggtgct ggaccgcccg gaaccagcgc aacagggcaa ggtcgtcttg
      481 ctcaccgtca atcttgacaa gagcgacctg ccgctccaga cggcctttcc gatcatggtc
      541 acgaacttgc tcaactggtt cggcgggacc aagggcgaat tgcgcgaggc gctcgccgct
      601 ggctcggttg ccgaggtcga gcttcccgcc gacaaaagtg cggagtctca ctttgtgctc
      661 aaaagtcccg gcggtaccga aacgcccgtg gttgtgcccg cgggggcggc caaagtgacc
      721 gtcggcccgc tcgaccgcac cggtgtctgg cgcgtgttga agcgatcggc aaaaggcctg
      781 gtttctgcca aaaaaggcaa ggacgcgatc gacccccgcg acgtggtcgc cgagctggct
      841 tgtaacctgg ctgatcggcg cgagagcgac cttcgcccgg cggtcgagcc cgccgatcgc
      901 cggcccgggt tggccgcggg gttggccgtc aggccgatct ggtattatct gctggcgtcg
      961 gccttgatgc tcacttgctg ggagtggttc ctctatcagc ggaggtggat cgactgatga
     1021 tggccctgtt gccgatcgcg ttcgatttcc agctcgagct gacgcgcccc tggtggctct
     1081 tgggcttggc cgccctgccg atcttggtct atttcttcta tcgaactctg gtcgatttcg
     1141 cccgctggca gcgcgtccta tccctctggc tgcgcggcgt catcgtggtc cttttggcct
     1201 tggcgcttgc gggattgagc ctggtgcggc cgacccgaga gctgttcgtt gttttcgcca
     1261 tggacaggag cgccagcatc ggcgagcagg ggaaccgtgc gatcgacgac tttctgacca
     1321 aatcggctgc ccatgccggc tctaaccgtt ttgccgtgct cccgtttgca agcgagcccg
     1381 gggttgttcg caccagtgac gaagccctcg cagccttgaa gaactcgaaa gacgccgccc
     1441 cgaaaaagga acccgtcgcc ggcgcatccg cggccgagcc ggaagacgca cgcaggctcg
     1501 atcgcaaggg gaccgatctg gcggcagcgc tccaggtggc tgccgcggcg atcccgccgt
     1561 tttatgtccc gcgaatcgtg ctattatccg acggcaaccc gactaatggc gacgtgctca
     1621 aagtcgccgc gtcgctcaac ggcaaggttg atgttttcac atccactctg ccggttcgtg
     1681 atgaccccga ggttcagctc tcggccgtcg ccgcgccggc gcaggtcctt cagggcgagc
     1741 ccttcaaagt cgaggtcttg atcgacacca atcacgacga cagcgagggg caactcgagg
     1801 tctaccgagg agacatcaag gttgccgggc agccggtcgc gctcaagaaa ggcgaaaacc
     1861 ggattgtcct caaacagacc atcgaccagg gtggattgac cccgatcacc gcccggttga
     1921 agggttacaa ggacacgctc ctggacaaca atagcgagtt tgggctggtg tcggcggccg
     1981 gtaagccgcg ggtgctcttg gttgacagcg accctgacca ggccaagcat ctgacctggg
     2041 cactcgaaga acagaatatg caggtagacg tccgtccgcc gcgcggcgtt cccgacaacc
     2101 tggccgagct gcaaaactac gacctcctca tgctctcgaa cgtgccggcg accgcgctca
     2161 gtctccgcca gatggaggtg gcgcggacct acgtgcaaga tctggggggc ggcctgatca
     2221 tgttgggagg ggaccagtcg ttcggtctgg gaggctacta caagaccagc ctggaagaaa
     2281 tcctgccggt tcgcagcgac tttgaaaaag agaaggaaaa gccgagcctg gccatgatgc
     2341 tggtgatcga taagtccggc tcgatgggcg gcgagaaaat cgagatggcc aaagaggcgg
     2401 cgcgcgccgc cgtcgagctc atggggccaa gcgacaaggt cggcgtgctc gcgtttgaag
     2461 gcgagaattt ctgggtcagc gagctgcacc catgctccga taaaggcttc gtgcttaacc
     2521 gcatcgctgc cctcgaggcc gggggcggga ccgatatggc tccggcgatg gaggaagcgc
     2581 atgagacatt gcgcggggct gtggccaagc tcaagcacgt gattgtgttg accgacggca
     2641 tttcgaggcc gggcgatttc gagggcattg cccaggggat ggccgccgac cgcatcacct
     2701 gctcgactgt ggcgatggga ggcgacgccg atcaatcgct tttggaagag attgcccgcc
     2761 tgggaaacgg acgattttac gtcgccgacg acccggccca ggtcccccag atttttgcca
     2821 aagagacggt caccgcgagc aagtcggcga tcaacgagca gccatttgcg ccgacgatcg
     2881 tacgctcgac gcaggtattg agcgagatcc ggctcgacga ggcaccgttt ctgcttggat
     2941 atgtggtaac ccggcccaaa ccgacggcgg aagtcatcct ggcgaccgaa gcgggtgatc
     3001 cgcttttggc gtggtggcga tatgggttag ggatgacggc ggcgttcacc tcggacgcca
     3061 aggcgcggtg ggccgcggag tggctaagct ggccccagtt cggccagttc tgggcccagg
     3121 tggtccggca ctcgttgcgc cgagcggaag ccaaggggac gctcgttcaa gtcgagcgtg
     3181 ccggccgcaa atccgtcgtt tcgatcgacg ccatcgagcc gtcgggcaag ttcttgaacc
     3241 aggcggcgac cgatctgacg ctggtcgacc ccaagctggc gacccggaag atcgaaatgg
     3301 cacaggtcgc gccggggcga tatcaggccg agttcgatac gacccagccg ggctcgtacc
     3361 agttgatgtt cagtcagacg cgcgatagcc agctcctggg ccgccagacg cgcggattgg
     3421 ccgttggcta tcccgatgag ctgcgattgc ggccggccaa cgccgagtta ttgaaatcga
     3481 tcgccggggc ctcgggcggg cggtttgacg ccaagccgga gtcggttttc gatgtgcctg
     3541 accgctctgc gccgagggct gtaccacttt ggccctacct ggctacggcc gcggcgctat
     3601 tgtttgtgct ggacgtggcc ttgcgtcgcg tcgacctgac gctgttgctt ccgcggcggc
     3661 ggcagatttc gctttccacg cgggcgtttt gatcgtacaa tcgtcgtgcc tttcccagtt
     3721 cggctctgtc gtcattcgcc ggtcctggtg acccggcggg aaaaagggag cagaagccat
     3781 gagcgagcgg atcctggaaa agctggagcc ggtacgccgg cggcaacaag gtttggaggt
     3841 catgcggtcc acggccgcgg gtctactggc cagttcgctc gtgggcgttg gactcggatt
     3901 gttgcggtgg caaggggcag gtgtaacagg agccggccga tggtggctgt gggcagccgc
     3961 catcgcggtg gcggggccag cgctcggtgc gatcgtgggt ctggcgcgcg gccgatccgc
     4021 tctgctcgcc gcggctgcgg tcgatcgtca gtatgggctc aaggatcgcg cactttccgc
     4081 ggtcgatttc atacgccgcg gcaagtcgac gtcgttgcac acgcttcagg tggctgacgc
     4141 cctggagcat ctggccggtc tcgatcctcg ccaggtggct ccgttccgga tgccggcggt
     4201 cttgccctac gcggtggcta cgatggcact ggctcttgtg ctcttgctct ggccgcgggc
     4261 cggggtggta caggcggagc cggccgcgcc gctcgaggcc gtgctggccg ccgccgagga
     4321 tgccgaagat ggcctggaag agctgaacga ggctgccaag aagaatgacg acatcaagct
     4381 gaaagaattg gtgcagaagc tgagcgagac gatccaggaa atgaagcttc ccggggtcga
     4441 caccaaagaa gctctcgcca agctttcgga gatgcaggcg gccatcacgg cccagcaggc
     4501 ccagttcaac gtcgggctgg tcgatgccca gatgcaatcg ctcggcgagg ccatggcctc
     4561 gacccaggcg ctggaagggg ccgggcaggc tctacagcaa ggcaagtacg acaaggccgc
     4621 cgaacaactg gaaaaagccg agcccaagtt tgatcgcaag gaaaccaaga cgttgaagga
     4681 aaagctggct caggccgcca aggcgatgga ggatggcggg ctgaccgagc tctccaccgc
     4741 cacgaccgag ctcgccgcgt cgctcgacga cgaagggacc gcccagggcg cctgcaagaa
     4801 actggggaac ctcgcccgca gccagggccg ccgcaagcgg atcgccgacc tgttgactct
     4861 tgctaccgtg aagctcggcg agtgcaaagg taattgcgat aagaatggcg gcgccaagct
     4921 tcgtcttcgc aagaagtcgg aaacgccttc cagcaagtgg ggactggcca taagcggcaa
     4981 cactgacggt gaaaagacca agctcgacgc ggcgcgcaag cgcgagcaga tccagggtca
     5041 gatgggtgaa ggtgattcgg aaaccgagac gacccacatg cctgaaggcc ggcagaccgc
     5101 ggcgcgcacc taccgcgagc agtatcagaa ataccgccgc atgactgagg ccgccctcaa
     5161 ttccgagccg attccgctcg gtcatcgcca aacgatccgg cggtatttcg agctgattcg
     5221 cccgcagaac gatgaagccg agaaggcgga tgcgacggtt caacccggaa agtaaattgt
     5281 ggcggatgcc tttccccccc ttgtgaaggg gggaaagagc agccacaaag gcgcacatta
     5341 tgattcggcc gctattgtcc caataactcg agcgtccgct taacgaacgc cgatgaggcc
     5401 tcgacggcct ccgcgggcga catcttgaga cgctgggccg tctcgaacct gcgtaaat
//
LOCUS       DATAQL010000790         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_997810, whole genome shotgun sequence.
ACCESSION   DATAQL010000790 DATAQL010000000
VERSION     DATAQL010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997810"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2320
                     /locus_tag="VHS97_13785"
     CDS             <1..2320
                     /locus_tag="VHS97_13785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449326.1"
                     /translation="FVRELTENGLAETADVESLREQLLKRSGAHDAPALARELVRYER
                     LTPYQAAAVLRGKTKGLLIGNYVVLDRLGAGSMGIVFKARHRVLKRIVALKLLPPSLA
                     RDPSAVQRFHREAKAAATLSHPNVVAVLDADEFRGLHFLVMEYVEGRDLARLVREQGP
                     LSLSQAIDCITQAARGLKAAFEASVVHRDIKPSNLLLEPGGTLKILDMGLARLDAVGG
                     PFGANQPDASLTQSNILLGTIDYMSPEQATNPRNADHRSDIYSLGCTLHYLLTGRPPY
                     SGETLIERLIAHREQPIPRLSDSLPEVSPSLDALLRRLLAKSPTDRFSTLDDLIAGLE
                     ACQPSAGTDINEWPALQIVTDPREQSGKRRAPIAAVVACVAAAAGILATGIYLSRRES
                     APSSQEPPPVPATPVSVTENVPAPEPDVRSPQRPLPTADQRQPEPNPIAAIASPARGI
                     PGKAILPADAVGLVREFRGHIRRVNGIAVSGDGLLALSGGQDRTVRLWDVATGNELRR
                     VDHDGPVDAVAITAHGRYGLSGSDDKTVRLWEFRPGNTVGMRRLDGHTRAVFAVAFAL
                     GDQFAVSGGADKTVRVWDLATGQISGSPLLHESPVVALAATGDDAVLAGCDDGTLWLW
                     DLMSRQRVRRLKAPGPVLCVAGSPLGHRALSGHADGVLVVWDLDIGAEIGRMVGHNDL
                     VRCTALLPDGRRALAGSQFGNLTLWDLDKRRELRRFSLAASSSDHAGQLGVAILADDV
                     HALTADTDATVRLWSLPASDASTDTAGQRPAP"
     gene            complement(2328..>3093)
                     /locus_tag="VHS97_13790"
     CDS             complement(2328..>3093)
                     /locus_tag="VHS97_13790"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX3449327.1"
                     /translation="DTLGVKGVQLLESRGEVIRLFASQREDYLRIVKALKDAIDQADK
                     ENRIGTIDGQTQKSIGGRSGGERGSDQITSTEPFKTRLREFEKSIHSAQVPIDADKSI
                     HWLDATLNGKSRKRMIVDFGINEIRVSARLASEVGVRSESGDQTVEIATVDGRIIRAR
                     PARLETVQVGPFTHHDVDCVVLPESAGDFPGVLGSGFFDQFSTRIDADARAIVLTQVQ
                     VKPILHPNKASSAKSAVSSKIRTTAPAPASGRSPAN"
BASE COUNT          542 a         1013 c          948 g          590 t
ORIGIN      
        1 atttgtccga gagctcaccg agaacggact ggcggaaacg gctgacgtcg agtcgctccg
       61 cgagcagctc ttgaagcgga gcggtgcaca cgacgcgccg gcgctcgcca gggaactcgt
      121 ccgatacgag cgcttgactc cataccaagc cgccgccgtc ttgcggggta agacgaaggg
      181 gctcttgatc ggcaattacg tcgtcctcga taggctgggt gcgggcagca tgggaattgt
      241 tttcaaggcg cgccatcgcg tcttgaagcg cattgtggcc cttaaattgc tgccgccttc
      301 cctggcccga gacccctcgg cagtacagcg ctttcaccgc gaggcaaagg ctgcggccac
      361 gctctcccat cccaacgtcg ttgctgtgct cgacgccgac gagtttcgtg ggttgcactt
      421 cctcgtgatg gagtatgtcg aagggcgcga cctcgcgaga ctggtacgtg agcaaggtcc
      481 cttatctctg tcacaggcca ttgactgcat tacccaagcg gcccgcgggc tcaaggcggc
      541 gttcgaggcg tcggtcgtcc atcgcgatat caaaccgtcg aacctcttgc tcgagcccgg
      601 cgggacgctc aagattctcg acatgggcct ggctcgcctc gacgcggtcg gcggcccctt
      661 cggcgccaac cagcccgacg ccagtctgac tcagagcaat atcttgctgg gaacgatcga
      721 ctacatgtcg cccgaacagg caaccaaccc gagaaacgcg gaccatcgct cggacatcta
      781 cagcctcggt tgcacgctgc attacctgtt gacgggccgc ccgccgtact ccggtgaaac
      841 tttgatcgaa cggctcattg cccaccgcga acaacctatc ccgcgtcttt ctgacagtct
      901 gcccgaggtt tcgccctctc tcgacgcact cctgcggcgt ctcctggcca aatcgccgac
      961 cgaccgattc tcgacgctcg acgatctgat cgccggcctc gaggcatgcc agccgagtgc
     1021 cgggacggat atcaacgaat ggcccgccct acaaatcgtg accgatccac gggaacagag
     1081 cggaaaacgg cgagcgccga tcgccgccgt cgtcgcctgc gtcgcggcgg cggcaggaat
     1141 actagcgaca ggaatttact tgagtcgccg agaatcggcg ccgagcagcc aagagcctcc
     1201 gccggtacca gccacgcccg tctccgtgac tgaaaatgtg ccagcgccgg aacccgatgt
     1261 gcgtagcccc caacgtcctc taccgaccgc ggaccagcgg cagccggaac cgaacccaat
     1321 cgccgcaatc gcgtcgccgg cgaggggtat tcccgggaaa gcgatcctac ccgccgatgc
     1381 cgtgggcctt gtgcgcgagt tcaggggaca tattcggcgc gtcaacggga tcgccgtttc
     1441 gggtgacggc ctgctggctc tctccggcgg acaggatcgg acggtccgcc tgtgggatgt
     1501 cgccactgga aacgaacttc ggcgtgtcga tcacgatggc ccggtggatg ccgtggcgat
     1561 aaccgcccac ggccggtacg ggctctcggg gtccgacgac aagacggtca ggctttggga
     1621 atttcgaccg ggaaacacag tcggcatgcg ccgactcgac gggcatacaa gggcggtgtt
     1681 cgccgtcgcg tttgccctgg gagatcagtt cgcggtttcg gggggcgctg ataagactgt
     1741 ccgcgtctgg gatctcgcga cggggcagat ttccggttcg ccactcttac acgaaagccc
     1801 tgtcgttgcc ctggcggcga ccggtgacga cgccgtcctc gcgggttgtg atgacgggac
     1861 gctatggctt tgggacctca tgtcccgcca gcgggtccgc cgcttgaagg cgcctggccc
     1921 ggtcttgtgc gtcgccggct cgcccctggg tcatcgagcg ttatcggggc atgccgacgg
     1981 cgtcctggtc gtttgggatc tcgatatcgg ggccgagatc ggccgcatgg tgggtcataa
     2041 cgacctggtc cggtgcactg cgctcctgcc cgacggccgc cgggcgctgg ccggcagcca
     2101 attcggcaat ctgaccctct gggatcttga caagcggcgc gagctccgcc gattttctct
     2161 ggccgccagc agctccgacc acgcgggcca gctcggcgtc gccattctcg ccgacgatgt
     2221 tcacgctctg accgccgaca ccgacgcgac cgtgcgtctc tggagcctgc ccgccagcga
     2281 tgcatcgacc gacactgctg gccagcgacc tgcaccctga tggtgcatca attcgccggt
     2341 gaacggcccg aggcgggagc gggggccgtc gtcctgattt tggacgaaac cgcggacttg
     2401 gccgaagacg ccttgttcgg gtggagaatc ggcttgacct gcacctgcgt gagcacgatc
     2461 gctcgcgcgt cggcatcgat cctggtcgaa aactgatcga aaaagccgct tccaagcact
     2521 ccggggaaat cgccggcgct ctcgggcagc acaacacaat cgacatcgtg gtgcgtgaac
     2581 ggcccgacct ggactgtttc gagccgcgcc ggccgtgccc gaatgattcg gccatcaaca
     2641 gttgcgatct ctaccgtctg gtcgccgctt tccgaacgga ccccgacctc cgatgccagc
     2701 cgcgccgaaa cgcggatctc gttgataccg aagtcgacta tcatccgttt gcgggactta
     2761 ccgttcaaag tcgcgtcgag ccagtgaatg gacttgtccg cgtcaatggg aacctgtgcg
     2821 gaatggatcg acttctcaaa ttcccggagt cttgttttga agggctcagt cgaggtgatt
     2881 tgatccgaac ctcgttcgcc gcctgatcgc ccaccgatcg acttttgggt ctggccgtcg
     2941 atggttccga ttctattctc tttgtccgcc tgatcgatcg catcttttag cgccttcacg
     3001 atcctgagat agtcttcccg ctgcgaagcg aagagccgga taacctcacc gcgtgattcg
     3061 agcaactgca cccccttgac cccaagtgtg tcg
//
LOCUS       DATAQL010000791         5893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4204352, whole genome shotgun sequence.
ACCESSION   DATAQL010000791 DATAQL010000000
VERSION     DATAQL010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204352"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VHS97_13795"
     CDS             complement(<1..222)
                     /locus_tag="VHS97_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449328.1"
                     /translation="MATPTEEERLRRWRLVLGKESQTIPGRAGMDDGLAVNLDSDDAQ
                     MDKVLEALYDSDRSAGLGSSCPNVNRWLGD"
     gene            complement(280..2574)
                     /locus_tag="VHS97_13800"
     CDS             complement(280..2574)
                     /locus_tag="VHS97_13800"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HEX3449329.1"
                     /translation="MGMTVFGIRHHGPGCARSLRRALDESRPDVVVIEGPPDAEDLLP
                     WVSHGGLKPPVALLVWQVGEPRRAAYFPLAVFSPEWQALAWAAENNVPARFMDLPQSH
                     QLALDSARENEAKKGEEEKKARVHGTPDADPDSALPTWRTDPLALMAEAAGYTDHELW
                     WEDQIERRADATGLFAAIHEAMCGIRAEFPDVRAKDLEREAYMRKTIRSVAKEGFPNV
                     AVVCGAWHAPVLDADGIAGKRAGCKIKDDNEKLSGLPKVKTTATWIPWTYSRLTYKSG
                     YGAGIRSPGWYAHLWESNDEAPTRWLATAARLLRDKDLDASSASIIEARRLADALAAM
                     REIRSPGLAELNDAILAVYCHGELAPLKLIHTRLEIGDVLGEIPAEAPSVPLAQDIAK
                     LQTSLRLKPSTAVKRLDLDLRTENDLARSRLLHRLIVLRIPWGRLEQSGGKRSTFHEI
                     WIVEWQPEFALAVIDANIWGSTVETAATAKIVNDAGSGSDLVSLTALLETAILAALEA
                     AVDPLLAQIQAQAAVGADVRHLMDALLPMARVARYGDVRGTQAAHIEPILVGMFERSS
                     VGLSAACSALDDDAALRMVESMARVNEALQILNREDLQAEWRVCIDQLMRKVVHANVR
                     GWCCRLLLDARAISAEELYRLARLSLSPVNPPSECAAWATGLLRGSGMALMHQDALWQ
                     VFDRWLAELSAPVFVEMMPLLRRAFADFTAPERRQMGNKVKRLGSGLGVRPVAPFSPV
                     EDTDIDHERASRVLPILTHILGTK"
     gene            complement(2592..3725)
                     /locus_tag="VHS97_13805"
     CDS             complement(2592..3725)
                     /locus_tag="VHS97_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3449330.1"
                     /translation="MTKNQASRPSTPQSPASLADGKLRLHAEQEFSAELQALAEIDDK
                     QRPPHWKLSPWAVKTYLLGGKLASGFEVTAKYIGNERLIEIAVATLATDRALLLYGLP
                     GTAKSWVSEHLAAAISGTSTLLIQGTAGTDEATLRYGWNYARLLAEGPSRGALVESPM
                     LKAMEAGMICRVEEMTRMPGEVQDTLITILSEKTLPVPELNIEVQANKGFNVIATANN
                     RDKGVNELSSALLRRFNTVVLPVPDSLEDEVKIVNQRTEQLGRALELPVERPALEEIR
                     RVVTILRELRSGVTDDGKTKLKSPSGTLSTAEAISVVTAGMAISAFYGDGQLGAGDII
                     SSLVGAIVKDPVQDQLIWQEYVQTVIKERDEWKDLYRATRDLT"
     gene            complement(3755..5341)
                     /locus_tag="VHS97_13810"
     CDS             complement(3755..5341)
                     /locus_tag="VHS97_13810"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HEX3449331.1"
                     /translation="MNELAKIALVGTSKYAGPLPATEHLAVALGGLEDEDRERSLLLR
                     CGANAVYRLAGQRAIAGIEPTVPAPPETKRTASPGVSRLLQDAVTTGGNNLLIDLLRQ
                     MEARSIVLPPALLPLLLDSKDASVRRSLIPVLGERGAWLCRQNPDWSFFHPKADPQAR
                     VDLDELKHIWDEGLIDQRIVALAVLRRHDPLSARDWVAQVWAKEKAPVRVRLVESLET
                     GLCRDDEVFLESCLNDRSSAVAQSAARLLCRLPQSALAGRMRARAAAILGIERTGLIY
                     KAIKLVCTPPQQIDRDWERDGILKQAPPGVGERAFWTERVLAAVPPSHWQSQFGLGPQ
                     QLMGAVADEPFAEPIVAGWSEAAVSFAASDPASADWLMPLWTHLAQGFGALKDADRPS
                     ALMKMQALLAAMTRDAAEAAIANLIHLPAGWNDVDVLNCVTALRPPWSAHYSAAVLAA
                     ARRRIQSRASEAAYRWANMLATNACEIHVDAFPLALVPWEMATAEERVSWFAANIESD
                     LNKFVTTIEMRTRFMSELNA"
     gene            complement(5341..>5893)
                     /locus_tag="VHS97_13815"
     CDS             complement(5341..>5893)
                     /locus_tag="VHS97_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449332.1"
                     /translation="IDDTWIVASQWVDYQDRLAAQRSWVVGRQSGRVGLILQFSAARQ
                     PFSEVIVAGTEQRGTLVFYPGVSGQRAKFLTRQGPPSTLELRPPGHATIDRFLGAVAE
                     SLARQPWLSAFGGILHDVTLVPAGDAWFARDQQSKALRLVGQNHWNAMAVTGGHPSDL
                     AGEWDGYGLRVLGVFADGHYWSL"
BASE COUNT         1094 a         1908 c         1791 g         1100 t
ORIGIN      
        1 atcgcccagc caccgattga cgttgggaca cgagctgccc agccccgccg agcggtcgga
       61 gtcgtagagt gcctcgagca ccttgtccat ctgagcatca tcgctatcaa gattgaccgc
      121 cagtccatcg tccatcccgg cccttccagg aatggtctgc gactctttgc cgagcacaag
      181 tcgccaccgc cgcaaacgtt cttcttcagt cggagttgcc atggacgctg ttccttcagt
      241 atcgagaaac agaggatgcg agctcactgc gtcgagaatt cactttgtcc ccaggatgtg
      301 cgtgaggatc ggcagcacgc gtgatgctcg ttcgtggtcg atatcggtgt cttccacggg
      361 ggagaacgga gccaccggcc ttacccccaa gccggagccg aggcgcttga ccttgttgcc
      421 catttgtcgg cgctcgggtg ctgtgaagtc ggcgaatgcc cgccgcaaga gcggcatcat
      481 ctcgacgaac actggagcac tgagctccgc cagccagcgg tcaaagactt gccagagcgc
      541 atcttgatgc attagcgcca tgccgctgcc gcgcaagagg cccgtcgccc aggctgcaca
      601 ctcagacgga ggattcaccg gcgacaacga taaccgggcc aggcgataca actcttctgc
      661 cgagatcgct cgcgcatcaa ggagcagccg gcagcaccag ccgcgcacgt tcgcatgaac
      721 gaccttgcgc atgagttgat cgatacagac tcgccattcg gcctggaggt cctctcggtt
      781 caggatctgg agcgcctcgt tgacgcgagc catgctttcc accatgcgca gagccgcgtc
      841 atcgtcgagc gccgagcacg cggcggagag cccgaccgag cttcgctcga acatccccac
      901 gaggatcggc tcgatatgag ccgcctgtgt accgcgcacg tcgccgtaac gggcgacccg
      961 cgccatcggc aagagcgcat ccatcagatg cctcacgtcg gctccgaccg ccgcctgtgc
     1021 ctgaatctgg gcgagcagag gatcgacggc cgcctctagc gcggcgagga tcgcagtctc
     1081 tagtagcgcc gtcagggaca ccaagtcaga gcccgacccg gcgtcgttga cgatcttggc
     1141 cgtcgccgcg gtctcgaccg tgctacccca gatattcgca tcgatgaccg caagggcaaa
     1201 ctcgggctgc cattccacga tccagatctc gtgaaacgtc gaccgtttgc cgccactttg
     1261 ctcgaggcgg ccccagggaa ttctcagcac gatcagtcga tgaagtagcc ggctgcgggc
     1321 caggtcgttt tccgtacgca gatccaggtc gagccgcttg accgccgtgc tgggcttgag
     1381 ccgcaacgat gtttgtagct tagcgatatc ttgcgccaag ggaaccgaag gcgcttccgc
     1441 ggggatctcg cccaagacat ccccgatttc cagcctcgtg tgaatgagct tgaggggcgc
     1501 cagctcaccg tgacaataga ccgcgagaat ggcatcattg agctcggcaa gcccgggcga
     1561 acgaatctct cgcatggctg ccagcgcgtc ggccaagcgc cgggcctcta tgatgctcgc
     1621 ggacgaagcg tcgagatcct tgtcgcgcaa cagccgcgcg gccgtcgcca gccagcgagt
     1681 tggcgcttcg tcattggact cccagaggtg agcgtaccac cccggcgaac ggattcccgc
     1741 tccgtagccg ctcttgtacg tcagccgcga gtaagtccat ggaatccagg tcgcggttgt
     1801 cttgaccttc ggcagaccgc tcagtttttc gttgtcgtcc ttgatcttac agcccgcgcg
     1861 tttgccggcg atgccatccg cgtcgagtac cggcgcgtgc caggcgccac aaacaacagc
     1921 cacgttcggg aagccctcct tggccacgga ccgtatcgtc ttgcgcatgt aagcctcgcg
     1981 ctctaggtcc ttggccctga cgtcgggaaa ctcggcgcgg atgccgcaca tcgcttcgtg
     2041 aatcgctgcg aacaggcccg tcgcatccgc gcggcgctca atctggtctt cccaccacaa
     2101 ctcatggtct gtgtaaccgg cggcctcggc catgagcgcc agcgggtcgg tccgccacgt
     2161 aggtaaggcg gagtcaggat cagcgtcggg tgtcccgtgc actcgagctt ttttctcttc
     2221 ctcgcccttc ttggcctcat tctcacgggc gctgtcgagg gccagttggt gcgattgcgg
     2281 caagtccata aaccgcgcgg gcacgttgtt ctccgcggcc caggcgaggg cttgccattc
     2341 gggtgagaaa accgccagcg ggaagtaggc cgcgcgacgc ggttcgccga cctgccagac
     2401 gagtagcgcc accgggggct tcaggccgcc gtgcgacacc cagggcaaga gatcttcggc
     2461 atcgggggga ccttcaatca caacgacgtc cgggcgcgat tcgtcgagcg cccgccgcag
     2521 gctgcgcgca cagcctggcc catgatgccg gatgccgaac acggtcatgc ccatcattat
     2581 gctccgagcg ctcacgtgag atcacgtgtc gcgcgataca ggtctttcca ttcatcgcgc
     2641 tctttgatga ccgtctggac gtattcctgc cagatgagct ggtcttgcac tgggtctttc
     2701 acgattgcac cgaccaagct cgaaatgatg tcgcccgcgc ctagctggcc gtcgccgtag
     2761 aaggcagaga tcgccatgcc agcggtcacg accgaaattg cttcggccgt cgacaaggtg
     2821 ccactgggtg atttgagctt ggtcttaccg tcgtcggtga caccgctgcg cagctcgcgc
     2881 aagatcgtga ccacgcggcg aatttcctcg agagcaggtc gttccaccgg cagctcgagt
     2941 gcgcggccaa gctgctcggt ccgttggttg acgatcttca cctcgtcttc gagagaatcg
     3001 ggcacgggca atacgacagt gttgaatcgg cgcaacaggg cgctcgaaag ctcgttgacc
     3061 cccttgtcac gattattggc cgtcgcgatc acgttgaatc ccttatttgc ctgtacctcg
     3121 atgttaagct cggggacggg cagggttttt tccgaaagga tcgtgatcag cgtatcctgt
     3181 acttcgccgg gcatgcgagt catttcttca acgcggcaga tcatgccggc ctccatggcc
     3241 ttgagcatcg ggctctcgac caaggcgcct cgcgacggac cctcggccag cagacgcgca
     3301 taattccaac catagcgcag ggtcgcctcg tcggttcccg ccgtgccctg gatcaagagc
     3361 gtcgatgtgc ccgaaatcgc ggccgcgaga tgttcgctga cccagctctt ggccgttccc
     3421 ggcaacccgt acagcaagag cgcgcgatcg gtcgcgaggg tggcgaccgc gatttcgatc
     3481 aaccgctcgt tgccgatgta tttagccgtc acctcgaatc cgctcgcgag tttgccgccc
     3541 aagagatagg tcttcacggc ccagggtgag agcttccagt gcggtgggcg ctgcttgtcg
     3601 tcgatctcgg ccaaggcctg gagctcggcg gagaactctt gttcggcatg cagccgcagc
     3661 ttgccgtcgg caagagacgc cggcgattgg ggtgtcgacg gtcgagaagc ctggttcttg
     3721 gtcacgcgtt gactccggaa aatggaactg acggtcaggc gttgagttct gacatgaaac
     3781 gagtgcgcat ctcgatcgtg gtgacgaact tattgagatc gctttcaatg ttcgcggcaa
     3841 accagctgac acgctcctcc gccgtggcca tttcccaggg gacaagagcc agcggaaatg
     3901 cgtcgacgtg aatctcgcag gcattcgtgg ccaacatatt cgcccatcgg taggctgctt
     3961 cactcgcccg gctttggatc cgccgccggg ccgccgccaa aacggcggcg ctatagtggg
     4021 cgctccaagg cggccttagc gccgtcacgc aattgaggac atcgacatcg ttccaccccg
     4081 ccggaaggtg aatcaggttg gcgatcgcag cttcggcggc atctcgcgtc atggcggcaa
     4141 gcagagcctg cattttcatc agtgcactcg gccgatctgc gtctttcaga gccccgaaac
     4201 cctgggctag atgcgtccag agcggcatga gccaatcagc cgaggccggg tcgctcgccg
     4261 caaaagagac cgcggcctcc gaccagccag cgacaatcgg ctcggcaaac ggctcgtccg
     4321 ccactgcgcc cataagctgt tgcggtccaa ggccaaattg gctttgccaa tgcgaaggcg
     4381 gcacggcagc aagcacccgc tctgtccaga aagcacgctc gccgactccg ggcggcgcct
     4441 gcttcagaat gccgtcgcgc tcccaatcgc gatcgatttg ttggggaggt gtgcagacga
     4501 gctttatcgc cttatagatg agtcccgttc gttcgatacc gaggatggcg gctgcccggg
     4561 cgcgcatgcg cccggcgagg gccgactggg gtaggcggca caagagccgc gccgcggact
     4621 gcgcgacggc cgagctgcga tcgttcaaac acgactcaag gaagacctcg tcatcgcgac
     4681 acaggcccgt ctcgagactc tccaccaacc tgacgcggac gggggccttt tctttcgccc
     4741 aaacctgcgc gacccaatcg cgagcgctca gagggtcgtg gcggcgaagg accgcgagcg
     4801 ccacgatgcg ctggtcaatc agtccttcgt cccagatatg ttttagctcg tcgaggtcga
     4861 cccgtgcctg aggatccgcc ttgggatgga agaacgacca gtcgggattc tgacggcaca
     4921 gccaggcacc gcgctcaccg agaaccggga tcaggcttcg gcgtacggac gcgtcttttg
     4981 agtccagtaa aagcggcagc agcgcgggtg gcaagacaat cgatcgcgct tccatttgcc
     5041 gcaataaatc gatcaggagg ttgttgccgc ccgtcgtgac ggcgtcttgc aatagccttg
     5101 acactccggg cgaagccgtt cttttggtct cgggcggggc aggaacggtc ggctcgatgc
     5161 ccgcaatcgc tcgttggccc gccaggcggt acaccgcgtt cgcaccgcat cgtagcaaga
     5221 gcgaccgttc gcgatcttcg tcctccaagc ctccaagggc aacggccaga tgctcggtcg
     5281 caggcaaagg cccggcgtat ttcgaggtcc caaccagggc aatcttggcc agttcgttca
     5341 tcaaagactc cagtaatggc catcggcaaa gacacccagc acgcgcaagc cgtacccgtc
     5401 ccactcgccc gccaggtcgg acgggtgacc acccgtcacc gccatagcgt tccagtggtt
     5461 ctgtccgaca agccggagtg cctttgattg ctggtcgcgc gcaaaccatg cgtcgcccgc
     5521 tgggacgagc gttacgtcgt gcaagatgcc accgaacgca ctcagccagg gctggcgcgc
     5581 cagcgattcc gcaaccgctc ccaggaagcg gtcgatcgtt gcgtggcccg gtggcctgag
     5641 ctcaagcgtc gacggcgggc cctggcgcgt gaggaactta gcgcgttggc cggacactcc
     5701 gggataaaaa acgagcgtcc cccgctgctc ggtgcccgcc acaatcactt cggaaaacgg
     5761 ctggcgtgcc gccgagaatt ggagtatcaa gccgacgcgg cccgactgcc ggcccacgac
     5821 ccacgatcgc tgagcagcca gtcgatcctg ataatcgacc cactggctag ccacaatcca
     5881 ggtgtcgtcg att
//
LOCUS       DATAQL010000792         4653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1455480, whole genome shotgun sequence.
ACCESSION   DATAQL010000792 DATAQL010000000
VERSION     DATAQL010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455480"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VHS97_13820"
     CDS             <1..1380
                     /locus_tag="VHS97_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3449333.1"
                     /translation="YHGGDFAGVRARLANLVDLGVTAIWLTPVYRPAPTWFQAKIGGA
                     PRRIADFHGYSPVDFFDTNPRFGTLLEFRGLVDEAHRLGLKVIQDHVLGHTGPKHRWR
                     ASPPTDDWFHGPLDRPPLCNFRFDALTNPHAREAERRGLTDGWFAGILPDLNMRNPNV
                     ARYAIQQSLWWTTMFEADGLRLDTYPMVDRAFWREWSRRVKVTHPAIRTVGEAWTVDP
                     ADLSFFQGGRTGWDGVDPGVDTVFDFPLYHAASAAFSGRAPARVLAESLRRDGLFPHP
                     ELLVTFLDNHDTPRLAAVPGVTTKRLMMAVAFLLTTRGIPQITWGDELGLPGHMDDRR
                     DFPGGFPGDPRDAFAAAGRTTAEQEVFAAYRDLLRLRKATPSLRGGTLTDLVATETVY
                     AYTRQRENERVVVALNLGKAPAEVMLPPDISGPAERLYGEARWFDAPGGPRLGMAAEA
                     AVVLRLTGR"
     gene            complement(1383..2156)
                     /locus_tag="VHS97_13825"
     CDS             complement(1383..2156)
                     /locus_tag="VHS97_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449334.1"
                     /translation="MSRRARLNPLFTVLLSLTLSPAVLAQSNQLPALRDWMEHQEEAA
                     KYLQNGQYSKAEERLNLAIKEIRPYLPDTRRIMAKSYCDLARVLYHQGRYADAEPLAR
                     WALSVRESDKKTKPDTVFQCVYVLGLIQSARKQHAEAEQLLLRSLALQEESLGRDHMN
                     SIIILNQLANVYVEQAKYADAEPLYLRSLAIHERKTPAEILDLAETAEKYAVLLRHMK
                     RYDDADRWHARAVTIRDTALSKAAKAKADQVSEQFKGYK"
     gene            2465..3703
                     /locus_tag="VHS97_13830"
     CDS             2465..3703
                     /locus_tag="VHS97_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3449335.1"
                     /translation="MWRFAWQNLITRPTRTFLAVMGLTIPIIAILGLFSLTNGIRTLM
                     GNTLARMNGLMVMRANSPAPVFSDLPESMVEDLRKIPGTRIVAPEVWKLSPQIEGRNL
                     LARAATKLLTGKGDDRFSSFAETIMIEGMQLPEHLNIKSGVFEQGLKKPEEGGGRFFT
                     MDDVGKPNILISDKIAHDFPNSDGTPKKVGQTLLIGGKPFNIIGIYETGSLLIDTTIV
                     MEITTARELLKLDKANVSVFYVEPQPLVELDDLSDRITKAFPDIQVKSMSQFNIQVGN
                     IMGKLDLFLLLTVGLALLVGGVGIANTMLMSAMERLVEFGVMRANGWTRSNILGLVTA
                     ESSLLGLLSGLVASVAAFGGIEILNSLISRFELRLDLTPLLVAASIGTAIIIATVAGL
                     YPAWRASRMTPMDAIRNEAS"
     gene            3690..4406
                     /locus_tag="VHS97_13835"
     CDS             3690..4406
                     /locus_tag="VHS97_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3449336.1"
                     /translation="MRPLENTAAESSAEVASSIIRVADVRKLFKSGDEEIEALRGVSF
                     EVPPGACSFFVGPSGSGKSTLLYLLGALDRPTSGTIEIGGQNLTAMNEVEQDVFRRQK
                     IGFVFQQFNLIPNLSAVENVLLPFIPTGLTPDCHTKAQNILRQVGLGHRLGHKPSKLS
                     GGQQQRVAIARALIKDPAVLLADEPTGNLDHKGGDEIFALLREQQEKRGCTLVVVTHD
                     RRFIRPDDHVLEIEDGRIAS"
BASE COUNT          870 a         1417 c         1416 g          950 t
ORIGIN      
        1 tatcacggag gcgatttcgc cggtgtgcgc gccaggctcg ccaacctggt cgatctcggc
       61 gtcacggcga tttggctgac accggtctac cgccctgcgc caacgtggtt ccaagccaag
      121 atcggcggcg cgcctcggag gatagccgac ttccacggct acagcccggt cgatttcttc
      181 gacaccaacc ctcgcttcgg cacgttgctc gaatttcgcg ggctcgtgga cgaagcccat
      241 cggctcggcc tgaaggtgat ccaggaccac gtcctgggcc acactggccc aaagcaccgc
      301 tggcgcgcta gcccgccgac agatgactgg ttccacggcc cgctcgaccg cccgcccctg
      361 tgcaatttcc gcttcgacgc gctcacgaac ccccacgccc gtgaagccga gcgccggggg
      421 ctgactgacg gctggtttgc cggcattctg cctgacctca atatgcgcaa cccaaacgtc
      481 gcgcgttacg caattcagca gtcgctctgg tggaccacga tgttcgaagc cgacgggctc
      541 aggctcgata cctacccgat ggtcgaccgc gccttctggc gggaatggag ccgacgtgtg
      601 aaggtgaccc atccggccat ccggacggtc ggcgaggcgt ggaccgtcga ccccgccgac
      661 ctgagcttct tccagggggg tcgcacaggc tgggacggtg tcgaccccgg cgtggacacc
      721 gtttttgact tcccactgta ccatgcagca tccgcggcct tttcgggccg cgcgccagcc
      781 agggtgctag ccgaatcgct gcggcgcgac gggctgttcc cacaccccga actcttggtg
      841 acctttctcg acaaccacga cacccctcgg ctcgccgccg taccgggtgt aaccacaaaa
      901 cggctcatga tggcagtcgc cttcttgctc acgacgcggg gcatccccca gatcacctgg
      961 ggcgacgagc tcggcctgcc cggccatatg gacgaccgcc gcgacttccc cggcggtttt
     1021 cctggcgacc ctcgcgacgc cttcgccgcc gcaggtcgta ccaccgcaga acaggaggtg
     1081 ttcgccgcgt accgcgacct gctgcggctc cggaaagcaa cgccctccct ccggggcgga
     1141 acgcttactg acctggtcgc gactgaaact gtttacgcgt acacacggca gcgcgagaat
     1201 gagcgtgtgg tcgtcgcgtt gaacctgggg aaggccccgg ccgaggtgat gcttcctccg
     1261 gacatctccg ggccggcgga gcgattatat ggcgaggcgc ggtggttcga tgcgccgggc
     1321 ggccctcgtc tcgggatggc ggccgaggcg gccgtcgtgc tccggctgac aggacgttga
     1381 ccctacttgt atcccttgaa ttgctcggag acttgatcgg ccttggcttt ggccgccttg
     1441 ctcagggcgg tgtcgcggat tgtcacggcg cgggcgtgcc atcgatcggc gtcgtcgtat
     1501 cgtttcatgt gccggagtaa gacagcatac ttttccgcgg tttcagccag atcgaggatt
     1561 tcagcgggcg tcttgcgctc gtgaattgcg agggatcgca ggtagagggg ttcggcgtcg
     1621 gcgtatttcg cttgttcgac gtaaacgttc gcaagttgat tgagaatgat gatgctattc
     1681 atatggtcgc ggcccaagct ctcttcctga agtgcgagcg accgcaggag aagttgctcg
     1741 gcctcggcat gctgttttcg cgccgactgg atcaggccaa gaacatagac acactgaaaa
     1801 acggtatcgg gcttggtctt tttgtcggac tcgcggaccg agagggccca tctggccagc
     1861 ggctcggcat cggcgtaccg tccctgatga tagagcaccc gagccaagtc gcagtagctc
     1921 ttggccatga tccggcgtgt gtctggcagg tagggccgga tctccttgat agcaaggttg
     1981 agccgttcct cggccttgga gtattgtccg ttttggagat atttggcggc ttcctcttgg
     2041 tgctccatcc agtcgcgtaa cgcgggcagc tggttcgatt gagcgaggac tgccggactg
     2101 agcgtgagtg acaataagac ggtgaaaagt ggattcaacc tggcacggcg gctcatgatt
     2161 gcaccctcca ggatgccgtt tcctccgatt ggcacgcggt tattgtgatt catagttgcg
     2221 agtggctatt gctcttttca aggggggaat tccggcaaaa agtcaatggc agccgtctcc
     2281 gtaccgccac gccacggcga tttccccttt aacgtgcgcg ggtcgtgcgg ctataaaaat
     2341 acgattgctt cgagttccat ggcgacatgc gtcgcgggtc tgaccgcctg actcgagttc
     2401 catgccacgc gaacgattgg catgggcgtt gcgggcgcag gcaataaacg attgagagcg
     2461 tctcatgtgg cgatttgcct ggcaaaactt gatcactcgg ccgacccgta cgttcctggc
     2521 cgtcatgggg ctgacgatcc ccatcattgc catcctgggg ctttttagcc tgaccaacgg
     2581 cattagaact ttgatgggaa atacgctggc gcggatgaac gggctgatgg tcatgcgtgc
     2641 caattcgccg gcccctgttt tcagcgacct tcctgagtcc atggtcgaag acctgcgcaa
     2701 gataccaggg acgcgtatcg tggctcccga ggtttggaaa ctttcccccc aaatcgaagg
     2761 ccgcaatctc ctggcccgag cggccaccaa gcttctcacg ggcaagggcg acgacaggtt
     2821 tagctcgttc gccgagacca tcatgatcga ggggatgcag ctgcccgagc atctcaatat
     2881 caagagcggc gtgttcgagc aaggattgaa gaagccggag gaggggggtg ggcgattctt
     2941 cacgatggat gatgtgggca agcccaatat cttgatcagc gataagatcg cgcacgattt
     3001 ccccaacagc gacggcacgc ccaaaaaagt gggacagacc ctgctgatcg gcggcaagcc
     3061 atttaacatc atcggcatct atgagacggg gtcgttgttg atcgacacaa cgatcgtcat
     3121 ggaaatcacg accgcgcgcg agctcttgaa actggacaag gccaacgtgt cggtctttta
     3181 cgtcgaaccg cagccgcttg tcgagctcga tgatctgagc gatcggatca ctaaggcctt
     3241 cccggatatc caggtcaaaa gcatgtcgca attcaacatc caggtcggca atattatggg
     3301 taagctcgat ctgttcttgc tcttgaccgt aggactggct ctcctggtcg gcggcgtggg
     3361 catcgcgaat acgatgctga tgagcgcgat ggaacgcctg gttgaattcg gcgtgatgcg
     3421 cgccaacggc tggactcgaa gcaacatcct ggggctggtg actgcggaaa gttcgctcct
     3481 ggggctcctg tccggcctgg tggccagtgt ggccgccttc ggaggaatcg aaatactcaa
     3541 ttcactaatt agccggttcg agctgaggct cgatcttacg cccttgctcg tggctgcaag
     3601 catcggcacg gcgatcatca tcgcaaccgt tgccggcttg tacccggcct ggcgcgcttc
     3661 ccgaatgacc cccatggatg ccatacgcaa tgaggcctct tgagaacacc gctgccgaat
     3721 ccagtgccga ggtcgcgtcc agcatcattc gcgttgccga cgtgcgcaag ttgttcaaga
     3781 gcggcgatga ggaaattgaa gctcttcggg gcgtttcctt tgaagtgccg ccgggcgcgt
     3841 gttcgttttt tgtcggcccg tccggatcgg ggaagagcac gctgttgtac ctattgggcg
     3901 cgctcgaccg cccgacctcc ggaactatcg agatcggcgg gcagaacctc accgccatga
     3961 acgaggtcga gcaagacgtc ttccggcgcc agaagatcgg ctttgtattc cagcaattca
     4021 acttgatccc caacctgtcg gccgtggaaa acgtgttgct gccgttcatt cctactggcc
     4081 tgacgccaga ctgccacacc aaggcgcaga acattctccg gcaggtcggc ctgggccatc
     4141 ggctcgggca caagccgtca aaactctccg gcggccagca gcagcgggtg gccattgcgc
     4201 gggcattgat caaagatccg gccgttctgc ttgccgatga gcccacgggc aaccttgatc
     4261 ataagggggg cgacgagatt ttcgccctct tgcgcgagca gcaagagaaa cgaggttgta
     4321 ccttggtcgt cgtcacccat gaccgccgct tcatccgtcc cgacgaccac gtcctcgaga
     4381 tcgaagatgg ccggatcgcg tcgtgacgcg ggacaaaaac tgggaaacga ccggccactg
     4441 ccctacaata gcccgtgccg atagccggcg agctggcctc acggaaccgt ttaaaacgac
     4501 ccgccgattg ttccctccac cgtttgaagg aatgtcgcga atgccaacga cgaagaaacc
     4561 gcaagagcgc ttgcgggccg cagtcccccg ttttgccctg gtcttgatcc cgtcggccgt
     4621 cctcgccctg gctggcttgg cagcttcgca aga
//
LOCUS       DATAQL010000793         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4204644, whole genome shotgun sequence.
ACCESSION   DATAQL010000793 DATAQL010000000
VERSION     DATAQL010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204644"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VHS97_13840"
     CDS             <1..538
                     /locus_tag="VHS97_13840"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:NF003768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX3449337.1"
                     /translation="GWLPRPIDDDLLRGVYDLAKMGPTSANTSPMRVIFVKTPEAKER
                     LKPALIAGNVEKTMAAPVCAIVANDLQFYEYIPRLYPQNPKFAELFTAPGKEEFTRTH
                     AFRNATLQGGYLIMAARALGLDCGPMSGFDNAKVDAEFFPDGRCKSNFLLNLGYGDRA
                     LLHPRNPRLEFDEACKFV"
     gene            573..1406
                     /locus_tag="VHS97_13845"
     CDS             573..1406
                     /locus_tag="VHS97_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX3449338.1"
                     /translation="MASPVEGPPREPVVDLEAYLARTGYTGRLDPSLATLQGLHVAHA
                     TSIPFENLDILLGREISLELDALESKLVASRRGGYCFEHNTLFSAVLDSLGFEVTRLA
                     ARVRFGSTAIRPRSHMLLSVKLDGQPWLVDVGFGGEGLLYPIRMLDPEESRQFGWTFR
                     VQRDGDIQVLQSLHGEGWFDLYAFTQEPQYPIDFVVANHFTATHPHSPFVQSLVVQRQ
                     NCETRFTLRNRELSEESPHRRNTRTLPDDDAVLAILAEVFGLTFPTGTRFPYRDQAGN
                     A"
     gene            complement(1479..2651)
                     /locus_tag="VHS97_13850"
     CDS             complement(1479..2651)
                     /locus_tag="VHS97_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEX3449339.1"
                     /translation="MRPRVALLVESSRAYGRGLLHGIAEYVRLHGPWSIYLAERGLGD
                     APPGWLDGWNGDGVIARIENRRIAQVVRRLALPTVDLRGLLTDLRVPLITTDDNGVAQ
                     TAFGHFLERGFRHFAFCGFLGADYSDKRSECFARLVEESGFVCHIYRSSRPPRMAGTE
                     ATEQRGWTTEAEVAQWIEQLPKPVGVMACNDIRAQQVLMACRLIGVGVPDEASVLGVD
                     NDELLCELSDPPLSSVVPDTHRIGFEAASLLDQMMRGEHTPQALTTIPPIGVLTRRST
                     DVLAIDDRIVSSAVHFIREHACDGITVEDVLAELPLSRSVFERRFAKIFGRTPKSEIL
                     RLQLDRVKQLLAETDIPLKQIAFKTGFRHPEYMSASFKERTGQTPGQYRRSSQRIT"
     gene            3174..>3475
                     /locus_tag="VHS97_13855"
     CDS             3174..>3475
                     /locus_tag="VHS97_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449340.1"
                     /translation="MRILTNRRGFTLIELLVVIAIIAVLIGLLLPAVQAARAAARRIQ
                     CTNNLKQLGLAAQNYVSSYGTFPIGSPLMYDPQVGYFSESQSVFVSLLGQLEQQPL"
BASE COUNT          687 a         1044 c         1015 g          729 t
ORIGIN      
        1 cggctggctg cctcggccaa tcgatgatga ccttttacgg ggagtctacg acctcgcgaa
       61 gatgggaccg acgtccgcca acacgtcgcc gatgcgggtc attttcgtca agacgcctga
      121 agcgaaggaa cggctcaagc cggcattgat cgcgggcaac gtcgagaaaa ccatggccgc
      181 ccccgtatgt gccattgtcg ccaatgactt gcagttttat gaatacattc ctcgtctcta
      241 cccgcagaac cccaagttcg ccgagctttt caccgcgccg gggaaagagg aatttacccg
      301 cacgcacgcg ttccgcaacg ccacgcttca aggtggctac ttgatcatgg cagcgcgtgc
      361 tctgggtcta gattgcggtc cgatgtctgg attcgataat gccaaggtcg acgccgagtt
      421 tttcccggac ggccgctgca agtctaattt tttgctcaat ctcggttacg gcgaccgcgc
      481 cttgcttcat cctcggaatc ccaggctcga attcgacgaa gcatgcaagt tcgtgtagta
      541 aaagaccaga tcgaacccac aaggtttcac ttatggcaag tccggttgaa ggtcccccgc
      601 gcgagccagt cgtcgatctc gaggcgtatc tggctcgtac cggctacact ggccgtctcg
      661 atccatcgct cgcgacgctc cagggtctgc acgtcgccca cgcgaccagc atcccgtttg
      721 agaatctgga catcctgctc ggccgtgaaa tctcgctcga gttagacgcc cttgaatcaa
      781 agctggtcgc gagccgccgc ggaggctact gctttgaaca caataccctt ttttcggccg
      841 ttctcgactc gctcggcttt gaagtgactc ggctggcggc ccgggtccgg tttggctcga
      901 ccgccatccg gcctcgttca cacatgctcc tttccgtcaa gctggatggc cagccctggc
      961 tggtcgacgt cggattcggt ggtgagggat tgctgtaccc aatccgcatg ctggatcccg
     1021 aagaatcgcg acagtttggg tggacttttc gggtccagcg cgacggagac attcaggtac
     1081 tacagtccct tcacggcgaa ggatggtttg atctctatgc atttactcaa gagccgcagt
     1141 atccgataga ctttgtcgtg gccaatcatt tcacggccac gcatccgcat tcgccgtttg
     1201 ttcaaagtct cgtagtgcag cgccaaaatt gcgagacacg ttttaccctg cgaaaccgtg
     1261 agctcagcga agaatcgccc catcggcgga acacccgaac actgccggac gatgatgccg
     1321 ttctggcgat cctcgccgag gtctttggac tgacctttcc caccgggacg cggtttccgt
     1381 atcgagatca ggccggcaat gcgtgatggc ttcgacggca acgatccgcg aagaaatgcc
     1441 cacaatgggc gagttcgtga ccctgaccgt cccatccatc acgtgatccg ttgggaagac
     1501 cggcgatact ggccgggcgt ctggccggtt ctctccttga aggaggcgct catgtactcg
     1561 ggatgccgaa agccggtctt gaatgcgatc tgcttgagag gaatatccgt ctcggccagc
     1621 agttgcttaa cccgatccag ttgcagccgc agaatttcgg atttgggggt gcgaccgaag
     1681 atcttggcga atcgtcgctc aaagacgctt cgagacaaag ggagctcggc gagcacgtct
     1741 tcgacagtga tcccgtcgca ggcgtgttcg cggatgaagt ggaccgccga agaaactatg
     1801 cgatcgtcga tggccaggac atcggttgat cgtcgcgtca gcaccccgat gggtgggatc
     1861 gtcgttagtg cctggggggt atgctcgcct ctcatcattt gatccagcag cgaggccgcc
     1921 tcgaacccga tccggtgtgt gtctgggacg acgctggaga gcggagggtc cgacagctcg
     1981 cacaacaatt cgtcgttgtc gacaccgaga acagaggctt cgtcggggac gcccacgccg
     2041 atcaagcggc aggccattaa gacttgctgg gcgcggatgt cattgcatgc catgacgccc
     2101 acaggcttgg gtaattgctc gatccactgc gccacttcgg cctccgtcgt ccagccgcgc
     2161 tgttccgtgg cctcggtgcc ggccatgcgc ggtggccttg acgagcgata gatgtggcac
     2221 acgaagcccg attcctcgac cagacgggcg aagcactcgg atcgtttgtc cgagtagtcg
     2281 gcgcccagga agccgcagaa ggcgaaatgg cggaacccac gctccaggaa atggccgaac
     2341 gccgtttggg ccacgccgtt gtcgtcggtg gtgatcagtg gcacgcggag atcagtgagc
     2401 agcccgcgca ggtccacggt gggcagagcc agccgcctga cgacctgggc gattcggcga
     2461 ttctcgatgc gagcgatgac cccgtcgccg ttccagccgt cgagccagcc cggcggcgcg
     2521 tctcccagac cgcgctcggc gagataaatc gaccatgggc cgtggagacg cacgtattca
     2581 gcgatgccgt gtaacagccc gcggccgtaa gctcgcgacg attccaccag caatgcgaca
     2641 cgaggacgca tcaccatctt cgatcgcctc cgaacctgaa agaatactca atccataatt
     2701 gagagtttcg catcataatc ggatataggc tatcgcctaa ccttgatcga gaaccggttt
     2761 ccgccaagta tctcaacaat cagcattgtc gtcttgcgcg aaaaaactca ataagttgac
     2821 gcaaagcttc aatgacgacg gcggttctcg ggtgtaacat gatcaaaggt gcgagagtgc
     2881 ggggcgagga tcgtcatctg ttgatgcaac cgatcgatat tgccgaccct cggtcatgca
     2941 agaacaggct gtcgactcac atcgtctctc gaccgacatt ttctcttttt tttctcgttc
     3001 gagaattggt cgtgcgcatg aggaggcttg gcgttgggaa cacatccaac gacgaatcgc
     3061 cggcgcctat gctctcgccg atcctcgatc ccgaggctcg tggggctgtg ggcggctgca
     3121 catctagacc gaccgcctcg cgcaccaagt caccagggac ctggaggttt cacgtgcgga
     3181 tactcacgaa tcgtcgcggc tttactctca tcgagttgct ggtcgtgatc gcgatcattg
     3241 cggtgctcat tgggctttta ttgcccgcgg tgcaggcggc gcgcgcggcg gcgcggcgca
     3301 ttcagtgcac gaacaacctc aaacagctcg gcctcgcggc gcagaactac gtttcgtcct
     3361 acgggacatt cccgatcggc agcccgctga tgtacgaccc gcaggtcgga tacttcagcg
     3421 agtcgcagag cgtcttcgtc tcgctgctcg gccagctcga gcagcagccg ctgtt
//
LOCUS       DATAQL010000794         5505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2982779, whole genome shotgun sequence.
ACCESSION   DATAQL010000794 DATAQL010000000
VERSION     DATAQL010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2982779"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VHS97_13860"
     CDS             complement(<1..512)
                     /locus_tag="VHS97_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX3449341.1"
                     /translation="MPAEILDGRALAQQLRRQVAGQVAELASQTGVVPGLAVVLVGDN
                     TASQVYVRNKEAACKAAGINGVVVRLDAKTSRAELLTTIDRLNEDPRVHGILVQLPLP
                     MQADESAVVERVAPLKDVDGFHPFNFGLLAAGTPRFVPCTPLGIRALLIHSGIETRGT
                     HAVVLGRSQIV"
     gene            complement(2793..5228)
                     /locus_tag="VHS97_13865"
     CDS             complement(2793..5228)
                     /locus_tag="VHS97_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3449342.1"
                     /translation="MRLSGSVVDITDLKRAEVALRESEERFRGTFENAAVGIAHKDIT
                     GRFLLVNEKFCDIVGYTREELLTKTWQEITYPGDSAASRERYGPLMRGESISFSLEKR
                     YWRKDHSLVWADVSVSLQRDAAGLPAYAIAIVQDISERKRLEGELRHAKDAAEAGNRA
                     KDEFLANVSHEIRTPMNAVLGMTELALDTPLAADQRQYLNTVRSAADNLLGILNDLLD
                     FSKIEAGKLELNPADFSLRATLGDTVLTLATRAHKKGLELACHVQFHVPDALVGDASR
                     LRQVLLNLIGNAIKFTEEGEVVVGVEVDDNAAPDGQVGLRFAVSDTGIGIPAEKQETI
                     FRAFEQEDTSTTRKYGGTGLGLTIATRLLALMGGTITVESQPGRGSTFAFTARFGQKK
                     HSPERVTAVPPVALHGVRVLIVDDNATNRQILEEWLRGWQMEPVAWGDGLAALDDLWH
                     SAASGRPYALLLLDARLPDTDGLAVAAQIRKRSELAATRIILLTSRDLPGDVARFREL
                     QIDAHMLKPVQPDELLETIFQVMSLSKGDAQATWETAPAPVPAAVPLRILVAEDNEFS
                     AELLEQLLARRGHRVRLAHNGREALALTELEAFDLLLLDVHMPELDGFQVIQAIRERE
                     RASGGHLPVIASTARARKEDRERCLGAGMDDFLSKPVRAADLWAAIDRVVGARPPAER
                     PQPSLLDARVLLDVCGGNAAILEKIGQAFRDRVPGHLAAIRDALRDQDAIRVREEAHK
                     LSGTVAAFSTVAGHVASDLEDRAARGQLEEGTLLVEQLETMVGEIKQLSSGLSVETLR
                     HQVQLQAHAPLVK"
     gene            5307..>5505
                     /locus_tag="VHS97_13870"
     CDS             5307..>5505
                     /locus_tag="VHS97_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449343.1"
                     /translation="MLDFELFGLTGEKRFSRPGYRTSIIWMDRHRRGREVERGLGAVV
                     AELFPAIPQFHAARLENAVWYF"
BASE COUNT         1094 a         1665 c         1612 g         1134 t
ORIGIN      
        1 acgatctgag aacggcccag aacgaccgca tgcgtgccgc gggtctcgat tcccgaatga
       61 atcaagagcg cgcgaatacc gagcggcgtg cacggaacga accgtggcgt cccggccgcc
      121 agcaggccga agttgaacgg gtgaaatccg tcgacgtcct tgagcggcgc gactcgctcg
      181 accacggccg actcatcggc ctgcatgggt aacggcaact gaaccaaaat accatgaacc
      241 cgtggatctt cgttcaggcg atcgatcgtc gtcaggagct cggctctcga cgtttttgcg
      301 tcaagacgaa cgacgactcc attgatcccg gccgccttgc atgccgcttc cttgttgcgg
      361 acataaacct ggctggccgt attgtcgccc accaagacaa ccgccaaacc cggtaccacg
      421 ccggtttgtg aggcgagttc ggcaacttgg ccggcgactt ggcggcgcaa ctgctgggcc
      481 agggcgcgtc catcgagaat ctcggcgggc aagaaaggcc tccaggatgt cgcgtcagtt
      541 cctgcggctt tcggcgtgcg cctgggcgac gaattcccgt cgaacgccga atcttcgcag
      601 gacaggccgc gacgtgacgt gaccgggcgc gacaatcaat tcaaggcccg aaggtctcag
      661 aacggaggag gagggattcg gacccatagt accggaaacc ggtaccccgg ttttcgagac
      721 cggtttatac tatcgtgccg acacggaaaa acaaggcatt ttcgcgtctc gctgctctcc
      781 gaggtcctcg cgcatacgcc ttgccatatg cctccagaac attctgataa tagtcgaacc
      841 gaactcgcgt taatcggaga ggtttgactc ccttagtcca tctaagacct gccttgctcg
      901 catttacatc cgcattttac catttgcagg acgatcgtcg aagatgaatt ggagcatttc
      961 tggatcacaa tgattcccat caggcgaacc cccccctgca agttgaagac ggtcgtcaca
     1021 gcatcctaaa gatcgccttt tctcgccatt gctgcatcat ggcggaggaa ctctccagca
     1081 cttttgccgg caacccaacc agcttggatc catcgaccgt tcggatctat ctgccggccg
     1141 ccctgccagg ggaaatcccc gtcagccagc cgcgccacag ctcctgctat cgccggccgc
     1201 aggaaagctg ggagcgccaa ctcataatat tataacatat agttaatagt tcattaatcc
     1261 ctaagtgctg atcgacatgc agaggaattc acgaacccag ttctggatgt cgacatgtgt
     1321 cctctcatct gcttcgccgt tccttccaac gtgaacactc ggcgtccgac caccggatgt
     1381 ggaatctcgc tactcgtgcg ctgcgtgttc gttgtcacca ttggaacatt gagtcgaggg
     1441 gcaagtggcg gccggaactg caagtcctga aaggcatctg caagtcgatc tcagcgtcgt
     1501 ttcagttggc caagaggttg tgacggagcg agtgcccgcg gcaaggcccg gccggggact
     1561 cgtcgtccgc caggacgtct tgtaccgggc cgaccttgaa ctgcggcaaa aatcgacaat
     1621 gtggttgcgt gcgcgtacaa ctccgagaaa ctcggggctt ccatatatgc acttgttcag
     1681 ttttcgggcc agaccatagt aagttcgtag cgaaaggttt ttcacgcgca aaaacgagtt
     1741 acacgctcag gttttcgcgc ttcgcaaacg ttggatttgt acatcacttc aaggcgaagg
     1801 tgcaatgttt caaagcggtt cagcaactgg cggtaggtcg tccctgcgcg aacggcgcgg
     1861 aatgtctttt gaaaacggtg gaactttgaa cggtcgtctt cttattgttc cctacgcatc
     1921 gtaactccta tgaaacaaca aatgctcata tgcgcaattg ttatgatact aatacatggt
     1981 attacaaggc actatcaatc ctttgtttcg tctcgttgct tagcgacggc caatctgtgc
     2041 cacgataaat ataagattga tctccagttc gtccattgct accgggcgga aaacccgctg
     2101 acgtccatcg agactcaaac atcttccgtc tcagaggcag tcctcggtgc tcgtgaagtt
     2161 cgcctctgtg agtccctgcc gacgcagctt tcgtcatatc cccctgggat cttcaagagg
     2221 gcgggattga aacgaattgt catctgcaaa gagctgtcgc tacttggaga cgtctgtggc
     2281 gggctgacgc ggtcacagga aggaatcatc ttcttcaatt ctctcttgta tgatggaaaa
     2341 cgaagcaaaa atgatcctga aagatgtttt catcacgagc tctttcatct cctagatcac
     2401 gcaatggggt tcagtgacat caccaagtac gacgagctcg ccggattcga gagggatccc
     2461 gaatgggaag aactcaacga cgaaggtttc caatacgatc cacggtggag tatacattat
     2521 tcaagactcg aggcgacggc catcaaaccg ggtatctgga cctactactc attgtcctcc
     2581 ccagcagagg acaaagcagt gttgttttct tatatggtaa ttgcgtacga caaactcaag
     2641 gaactttcca agagagatga aattctagag cgaaagatta gcttgctgag tcggcgacta
     2701 ttgagctttg acgccttctt tgacgagaac ttctgggttg cgagagcgcg agcgggccgc
     2761 gaaaaccggg acaagacgag ggcgagtgcg tttcatttga ccaatggtgc atgcgcttgg
     2821 agctgtactt ggtgccggag ggtctccacc gacaagccgc tcgaaagttg tttgatctcc
     2881 ccgaccatcg tttcaagctg ttccaccagg agcgtgcctt cttcaagctg gccgcgagcc
     2941 gcacgatctt caagatccga tgccacgtga ccggcgaccg tggagaacgc ggccaccgtc
     3001 ccactcagct tgtgagcctc ctcgcgcacg cggatggcat cctggtctcg taaggcatcc
     3061 ctgatagcag ccaggtgacc tggcacacga tcccgaaagg cctgaccaat cttctcgagg
     3121 atggcggcat ttcccccgca gacatccaac agcacacgcg cgtcgagcag ggacggttgc
     3181 ggtcgctcgg ccggcgggcg ggcgccgacg acccggtcta tcgcggccca caggtcggct
     3241 gcgcggaccg gcttggacag gaaatcatcc attcccgccc cgaggcagcg ctcgcggtct
     3301 tctttccgtg cgcgggccgt cgaggcgatg acgggcagat gtcctcccga ggcccgctcc
     3361 cgctcccgga tcgcctggat gacctggaag ccgtcgagct ccggcatgtg gacgtcaagt
     3421 aacaagaggt cgaaggcctc gagttccgtc agggccagcg cctcccggcc gttgtgcgcc
     3481 agccgcacgc ggtggccccg ccgcgccagg agctgttcca ggagttcggc actaaactcg
     3541 ttgtcttccg ccacgaggat gcgcaacggc accgcagcgg ggaccggcgc cggggctgtt
     3601 tcccacgtcg cctgtgcgtc gcctttcgac aagctcatca cctggaagat cgtctcgaga
     3661 agctcgtccg gctggaccgg cttgagcatg tgagcgtcga tctgcagctc gcggaagcgg
     3721 gccacatcac cagggagatc cctggaggta agcaggatga tccgcgtggc ggccagctcg
     3781 gatcgctttc ggatctgggc ggccactgcc aggccgtccg tgtcgggcag gcgggcatcc
     3841 agcagcagca gcgcgtaggg ccggccgctg gcggcgctgt gccagaggtc gtccagggcc
     3901 gccagcccgt cgccccacgc taccggctcc atctgccagc cacgcagcca ctcctccagg
     3961 atctggcggt tggtcgcgtt gtcgtccacg atgagcacgc gcacgccgtg gagcgcgacc
     4021 ggcggcacag cggtaactcg ctccggcgag tgcttctttt ggccgaaccg cgccgtgaag
     4081 gcgaaggtgc tgccgcggcc cggctggctc tccacggtga tcgtcccgcc catcagggcg
     4141 agcagccggg tggcgatcgt cagacccaac ccggtcccgc cgtacttccg cgtggtcgac
     4201 gtgtcttcct gctcgaatgc gcggaagatc gtctcttgct tttcggctgg gatgccgatg
     4261 ccggtgtcgc tcactgcgaa ccggagcccg acctgcccgt ctggcgcagc attgtcgtcg
     4321 acttccactc ctacgactac ctcgccctct tcagtgaact tgatggcgtt gccaattagg
     4381 ttgagcaata cctggcgtag ccttgaagcg tctccgacca gcgcgtcggg cacgtggaac
     4441 tgcacgtgac acgccagctc tagacccttc ttgtgggcgc gtgttgcgag ggtcagcacc
     4501 gtatcaccca gcgtcgcccg caacgagaag tcggcgggat tgagctcgag cttgccggct
     4561 tcgatcttgg agaagtcgag taaatcgttg aggatgccca gcagattgtc ggccgcggat
     4621 ctcaccgtgt tcaggtattg ccgctggtcc gcggccaggg gtgtgtcgag ggccagctct
     4681 gtcatgccga ggacggcgtt catgggggtg cggatctcgt gactgacgtt ggcaaggaac
     4741 tcgtcctttg cccggttgcc cgcctccgcg gcatctttgg cgtgccgcaa ttcgccctcc
     4801 agccgcttgc gctccgagat gtcctggacg atcgcgatcg cgtaggcggg caatcccgca
     4861 gcgtcgcgct ggagcgagac cgacacgtcc gcccagacga gcgaatgatc cttgcgccag
     4921 tagcgcttct cgagcgagaa actgatcgac tcgcctcgca tcaaggggcc gtaacgttcg
     4981 cggctggccg ccgaatcacc agggtaagtg atctcctgcc aggtttttgt gagcagctcc
     5041 tcgcgggtgt agccgacgat gtcgcagaac ttttcgttga cgagcaggaa gcggcccgtg
     5101 atatccttgt gggcaatgcc gacggcggcg ttctcgaacg tgccgcggaa ccgctcttcg
     5161 ctctcgcgca gggccacttc ggcacgcttg agatcggtaa tatcgacaac gctgcccgag
     5221 aggcgcactg gccgaccttg agcatcccgt gtggctgcgg cgcgggatag aacccaacgg
     5281 cacgatccgt ctcggtggag aacgcgatgc tcgatttcga actcttcggc ctcaccggcg
     5341 aaaaacgatt cagccgcccc ggctaccgaa cctcgatcat ctggatggat cggcaccggc
     5401 gaggtcgcga agtcgagcgg ggtctcggag ccgtcgtagc cgaattgttc ccggcaattc
     5461 cacaattcca cgcggctcgt ctggagaacg ccgtctggta tttct
//
LOCUS       DATAQL010000795        10437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4661070, whole genome shotgun sequence.
ACCESSION   DATAQL010000795 DATAQL010000000
VERSION     DATAQL010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4661070"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2309)
                     /locus_tag="VHS97_13875"
     CDS             complement(<1..2309)
                     /locus_tag="VHS97_13875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3449344.1"
                     /translation="MNRATEYGMGRASEQILPQQPREATRRSPAKLRCVHRWIEAQAE
                     RAPAAVALTCAGETITYSQLNARANQLARRLCAQGVGPEVVVGLCAGRTAAMVVGLLA
                     VLKAGGAYVPLDPSYPAERLAFMLGDASATVLLTQADLLIRLPASSASIICLDRDWRS
                     IGAESDGNLSGGAGLQNLAYIIYTSGSTGRPKGAMINHLGLANYLSWCAKAYAVPDGV
                     GAPVHSSISFDLTITALLAPLIAGRRVDLLDEDLGIEQLSEALRHSRDYSLVKITPAH
                     LRWLGDQMDPRDAAGRTRAFVIGGEQLRPEHVSFWRQHAPDTTLINEYGPTETVVGCC
                     VYRVPRDQPISGPIPIGRPIANTRLYVANRDLEPVPVGVAGELYIGGAGVARGYLNRP
                     GLTAERFIPDPFGMEPGGRLYRTGDLARWRADGNLEYLGRLDRQVKVRGYRIELGEIE
                     EALGQHPAVRETAVLALEDAPDDRRLVAYLTVANDRPVPASFELRQFLRKSLPEPMIP
                     AAYVVLEALPLTPNGKVDREALPALERGARPPITLSAAPHGPVEEEVASVWATVLGLD
                     RVGAHDGFFDIGGHSLLATQVISRLRKTFGVEIPLRALFEAPTVAGLAERIEAIRRGG
                     ARREASPIKPTTELGPLPLSFSQEALWFLDQLTPGQPTFNVTAALRIKGPLDHGALER
                     SIKELGRRHEALRTSFVATGGTPQQVVDGHASLSLDTTDLTELPLELREDAARRRALD
                     ESRRPFDLTQGPLAHVSLLRLGDADHAVL"
     gene            complement(2302..3771)
                     /locus_tag="VHS97_13880"
     CDS             complement(2302..3771)
                     /locus_tag="VHS97_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX3449345.1"
                     /translation="MSVERDAPLPASFAQQRLWFLDQLEPGRASYHIPAAVTLVGRLD
                     IPALERALSEIVRRHETLRTTLVADGGVPRQVIAPRLELPLVIHDLSFLGDAAREAQD
                     RIREEAERPFDLARGPLVRAALLRLCEQEHIAVVTMHHAISDGWSIGILIRDLSALYQ
                     SFRLGEPSPLPEMAIQYADYAVWQRSWLQGAVVEAQLDYWTEQLAAVPDLDLPTDRPR
                     PPALSERGAERSTILPPTNLEAVRALGRREGATLYMTLMAAFQVLLSRYSGQEDIAVG
                     TPVAGRTHPELEGLIGVFVNTLVVRGNLSGNPGFRELLRRIRRTTIEAYTHQDLPFEK
                     LVTIAHRDRAAGRAPLFQVMFALQNVPLPALRVPELLITPVELSSRTSKFELTLFATE
                     VPEGLRLTMEYSTDLFDAARVDRMLAHYRILLEEIVIRPDQPIGAIRMLTEEERTQLL
                     VGWNPTAVDDLARLLDGPETNELDSLLYELSPAELATDE"
     gene            complement(3832..5652)
                     /locus_tag="VHS97_13885"
                     /pseudo
     CDS             complement(3832..5652)
                     /locus_tag="VHS97_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
     gene            complement(5670..>10437)
                     /locus_tag="VHS97_13890"
     CDS             complement(5670..>10437)
                     /locus_tag="VHS97_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3449346.1"
                     /translation="TGPPLSHGQQMLWYAHQFTTTGAAYHVLGAGKVRAELDKSAFRQ
                     AMRRVIVRQEALRTTFVLIDEKPAIRLLDLADLALREDEWLPVEDVPGCDDGALHERL
                     AELARRPFDLERGPLFRVHLLSRSASEHVFLLVFHHIIADFWSTAVFLDNFKTAYTAL
                     CAGQDGELPPPVSSYAQFARWQHEMVVGEEGERHWKYWRDQLAQPLPVLDLSTDFARP
                     PIQSYKGAIKHYYLDPTLTRAIIALGESRGASLYTVLLAAFQALLARFSGQADIVVGT
                     PVAGRTLPGLQDLVGYFVNLIPMRGDLSGNPTFDEYLGRVRRTVSDGLEHQDFPFGLL
                     VHRLQGNPDPSRPPLFQVMFAHQKIQPLDDQGLAPFALGVPGARLDLHGLAVESIAFE
                     RQTALFDLTMMTARDGDRLCVALEYSTDLFKSSTIDRMADGFRNLLESIVADPGRRLA
                     DLALFSGSERHQLLGDWADAPAIPYEDIAIHHRFERQVELSPDAVALVSSEQSLTYRD
                     LNRLANLLADRLLGVGVRPETVVGLYLDRWTSRMVGLLGVLKAGGAYLPLDTDHPAER
                     LGAMLEDSGATVLVTEDHLRGRIPVCTPLVVTLESSGESLAGVEPENPRVPVDPENLA
                     YVVFTSGSTGRPKGVMVSHRSLLAITAAWEHAYDLCQPPLRHLQAAGFGFDVFTGDWC
                     RALTTGGTLVACPRTVLLDPAALSNLIQRERIECLELVPALADALATHLELQGEDLRG
                     VRLLAVGSDTVRGRLYRRLGALVGPGARVVNSYGLTEATIDSTYFSGPPQGLEGEDGP
                     VPIGRPFPGTRTYVLDRRGEPVPVGVAGELYIVGSGVARGYLSNPRQTAERFVPDPHG
                     GRGTRMYATGDRARWRDGGVLELLGRRDGQVKVRGFRVELAEVEAVLARHPSVSETVV
                     VDVADALGDKRLAAYVVPAALSVPTAMDLRRWLKERIPEPMVPSSYVFLDSLPLSPNG
                     KLDRSALPPLAALEVDGSATAYVPPRTGAEEILAGIAADLLGRSRVGIHDNFFEIGFD
                     SILGIQMVSRARQAGLAFGPAHLFRHPNIADLAAAVESSSDHQGSSRISTPAIAPFEL
                     TPDGIDLEAVKRAFADDGGIEDLYPLTPMQEGMLFHTLADPEAGHYVEQFVCRIQGEL
                     RPAALQESWNRLVARHPALRSTIHWIDFDRPYQVVHRRADQPLEYQDWRGLTPAEQEE
                     RLTAYLGSDRRRGFIPSKPPLSRLAVFRLSETAHECIWSIHHAVIDGWCLSILLHEFL
                     DIDEAIRLEREPALQPMRPFRDYVAWLRNRDDEKAEGYWRQVLRGFTSATQLGLDGCS
                     PGRRGTLRECAVERETVLPADVTARLQAVGRSNRLTLSTLIQGAWALLLSRYSGRSDV
                     LFGVTVSGRPPELSGVELMVGMFINSLPLRVQVIEEAFLIPWLHELQSTMIELRRFEA
                     IPLSQIQSWSEVPAGIPLFESIVIVQNLPYVASLQERADRLGIESARYLERTHYPLAV
                     TVVPGTELGIKIGFDSDRFDPSAIEQTLGHLRTVLEAMAAQPEQRLVDLPLMTPNEHE
                     QLIGQWNRSESEPLPDDLHLDQFTESALDALIDRLR"
BASE COUNT         1938 a         3279 c         3472 g         1748 t
ORIGIN      
        1 aagagaactg cgtggtcggc gtcgcccagt cgcagcagac tcacgtgagc aagtggcccc
       61 tgcgttaagt cgaagggccg ccgcgactca tcgagcgctc gacgccgggc cgcatcctcg
      121 cgaagctcga gcggcagctc agtcaggtct gtcgtatcca gtgataacga tgcgtgtccg
      181 tccacgacct gctgaggtgt gccgcccgtt gcaacgaaag acgtccgcag cgcctcatgg
      241 cgacggccaa gctccttgat gctccgctca agagcgccgt ggtcgagcgg ccctttaatt
      301 cgtagggccg cggtgacgtt gaaggtgggc tggccgggcg tgagctgatc gaggaaccag
      361 agggcctctt gggagaacga gagcggcagc gggccgagct cggtggttgg cttgatcggg
      421 ctcgcctcgc gccgggcgcc gccgcgacgg atcgcctcga tccgctcggc cagtccggcg
      481 accgtcggag cctcgaatag cgcacggaga gggatctcca cgccaaacgt tttgcgcaga
      541 cgcgagatga cctgggtcgc caagagggag tggccgccaa tatcaaagaa gccgtcgtgg
      601 gcaccgacgc gatcgaggcc caacaccgtt gcccagacgg acgccacctc ctcctcgacc
      661 ggcccgtggg gcgcagcgga gagggtgatc ggtggcctcg cgcctcgctc gagagcaggc
      721 agggcctcgc ggtcgacctt gccgttggga gtcagcggca gggcctcgag caccacgtat
      781 gccgccggga tcatcggctc ggggagtgat tttctcaaga attggcgtag ctcgaagctg
      841 gccgggaccg gccgatcgtt tgcgacggtc agataggcga cgagtcgacg atcgtcgggc
      901 gcgtcctcga gcgccaagac cgcggtctcg cgcaccgcgg ggtgttgtcc gagtgcctct
      961 tcgatctcac ccagctcaat ccggtagccg cgaaccttga cctggcgatc caacctacca
     1021 aggtactcga gattgccgtc ggctcgccaa cgggcgaggt cccccgtacg atagaggcgt
     1081 ccccccggct ccatcccgaa tgggtcgggg atgaaacgct cggccgtcaa gcccggcctg
     1141 ttgagatatc cccgcgcgac cccggcgcct ccgatgtaca gctcacccgc aacgccgacg
     1201 ggcacgggct cgagatcccg gttcgcgacg tacagacgtg tgttagcgat cggccgcccg
     1261 atcgggatgg ggccggaaat gggctggtcg cgcggcacgc ggtagacgca gcagccgacg
     1321 accgtctcgg tcggaccgta ctcgtttatc aaggtcgtat cgggggcgtg ctgccgccag
     1381 aaggacacgt gctccggcct caattgctca ccaccgatga caaacgctcg cgttcggccc
     1441 gcagcatcgc ggggatccat ttgatcgccg agccaacgaa gatgagcggg agtgatcttg
     1501 acaaggctgt agtcgcgcga atgccggagt gcctcgctca gttgctcgat gcccaggtcc
     1561 tcgtcgagca agtcaacgcg ccggcctgcg atcaggggag caagcaaagc tgtgatcgtc
     1621 aggtcgaacg agatcgacga atggaccggt gcacctactc cgtcagggac ggcatacgct
     1681 ttcgcgcacc agctcagata attcgccagg cccagatggt tgatcatggc gccttttggt
     1741 cggccggtcg agcccgacgt atagatgatg taggcgagat tctgaagccc cgcaccgcct
     1801 gagaggttgc cgtcggattc cgccccgatc gacctccaat cacggtcgag gcagatgatc
     1861 gacgcggaac tggcgggaag tcgaatgagc aggtcggcct gcgtaagtag gaccgtggcg
     1921 cttgcgtcgc cgagcatgaa agcaagccgc tcagccggat acgacgggtc gagcggcaca
     1981 taggcgcccc ccgccttgag gacggccagg agccccacca ccatcgccgc cgtccggccg
     2041 gcgcacaggc cgaccacgac ctcgggtccg acgccttggg cgcagaggcg gcgggccagc
     2101 tggttggccc gggcattgag ctgggaatat gtgatggtct ctccggcgca ggtcagggca
     2161 actgccgctg gggcccgttc ggcctgtgcc tcgatccagc ggtggacaca ccgtagcttc
     2221 gccggcgatc gccgggtcgc ctcccggggt tgctgcggca gaatctgttc ggaagcacgc
     2281 cccatgccgt actcggtcgc cctattcatc ggttgcgagc tccgcgggag acaattcgta
     2341 gagcaaggag tccagctcgt tagtctccgg tccatcgagc aacctcgcga ggtcgtcgac
     2401 tgcggttggg ttccatccga cgagcagttg cgtccgctct tcctcggtga gcatccgtat
     2461 ggcgccgatt ggctgatcgg gacggataac gatctcttca agaaggatcc ggtagtgggc
     2521 caacattcgg tcgactctcg ccgcgtcgaa caggtcggtg ctgtactcca tcgtgagccg
     2581 caatccttcc gggacttcag ttgcgaagag ggtcaactcg aacttcgacg tccggctcga
     2641 gagctcgacg ggagtgatca gaagctcggg cacgcgcagt gccggcaacg gtacgttctg
     2701 gagggcgaac atgacctgaa agagcggagc gcggccagcg gcgcgatcgc gatgggcgat
     2761 ggtcacaagc ttctcgaaag gcaggtcctg atgcgtgtaa gcctcgatcg tcgtccggcg
     2821 gatgcgccgg agcaactcgc ggaagcccgg gttgccggat aagttgccgc gcacgacgag
     2881 cgtgttgaca aaaaccccaa tcaagccttc cagctccggg tgagttcgtc cggctaccgg
     2941 cgtcccgacg gcgatatcct cctggcccga gtaacggctc aagagcacct gaaaagctgc
     3001 catgagtgtc atgtacaggg tcgccccctc gcgtcgaccg agtgctcgca cggcttccag
     3061 gttggtcggc ggtaagatcg tcgaccgttc ggcgccgcgc tcgctcaagg ctggcggtct
     3121 gggacgatcc gtgggcagat cgagatcagg aacggccgcg agctgctccg tccagtagtc
     3181 gagctgcgcc tcgacgaccg cgccctggag ccagctacgc tgccagaccg cgtaatcggc
     3241 gtactggatc gccatctcag ggagcgggga cggctccccc agcctgaacg actggtaaag
     3301 cgccgagaga tcgcggatca ggatcccaat cgaccagccg tcggaaatcg catgatgcat
     3361 ggtcactacg gcgatgtgct cttgctcgca gagccgcagc agagcagcac ggacgagcgg
     3421 cccgcgcgcc aggtcaaacg gtcgctcggc ttcttcgcga atgcggtcct gggcctcgcg
     3481 cgcagcgtcg ccaaggaagc tcaggtcgtg gatgaccaat ggcaattcga gcctcggggc
     3541 gatgacctgg cgcgggacgc caccgtcggc gaccagcgtg gttcgcaacg tctcgtgtcg
     3601 gcgcacaatt tcgctgaggg ctcgttcgag cgccgggatg tccagtcgac ccacgagcgt
     3661 gacagcggcc ggtatgtgat acgatgcgcg acccggttcc agttgatcga ggaaccatag
     3721 ccgctgctgg gcgaacgatg ctggtaaagg agcgtcgcgt tcgaccgaca ccagcggggg
     3781 taccgcggga gatttcccgt cctccagagc ctcttcgaca atgcgtgcca gtcgcgcgac
     3841 ggtcggacaa tcgaggaaat ctcttagaga tgcctcgaca tcgaacgtgt ggcggatccg
     3901 tgacagaagc tgaacggcca aaagcgagtg gccgccgcgc tcgaagaaat tgtcgtgagc
     3961 accgaccgaa tggccgccga gcaactcggt ccagatctcg gccaaggctt cttcgatcgg
     4021 ccctcgcggt ggtacgaaat cggcacccgc tggcggccga gcgtgtctgg ggtcggggag
     4081 ggcctggcgg tcgatcttcc cgtttggcgt caagggtaaa gcgtccagga aaatgaacgc
     4141 cgaggggacc atgtgctcgg gaattgtttc caggagccgt cgtcgcagct cggccgcgaa
     4201 ggtcggatcg gattggtcac gcgggacgat gtacgcggcc aaactcatct ctccactggg
     4261 atccgtatat gccgcgacga ccgcgtcgct taccgctggg tgctcggcca acgcggcttc
     4321 gatctcgccg agctcgatgc gaaacccgcg gaccttgacc tggtgatcga cccgtccgag
     4381 acattcaagt cttccgtccg gccgccatcg ggcgaggtcg ccagaccgat aaaggcgacc
     4441 gccgggagga ttcccgaacg ggtcggggat gaaccgctcg gccgtcagac tagggcggtt
     4501 tcgatagccg cgcgccagcc cggcgccacc gatataaagc tcgccgacaa tgccgaccgg
     4561 gacggctcgc agccgtttgt ccaatacata gaggcgcgta ttggcgatcg ggctgccgat
     4621 cgaaatgggg gcctcgccgg cctcaacgcg ccaggctgtt gaccagacgg ttgtctcggt
     4681 cggaccgtag aggttccaga gagcagcacc cttgtcgatc aatcggtcgg ccagcgcgcg
     4741 cggaagtgct tcaccgccac agagcatcgt gagcgtcggc ttgccgcgcc agccgccttc
     4801 cagcaacatt cgccacgtgg caggcgttgc ctgcaggaaa gtgatccgcg ggtcgtcgag
     4861 gcggtcggct aaacgagcgc cggcggcggc gacctcgcgg tcgatcagat ccacgcgcgc
     4921 gccggcgatc aagggcaaga agttttcgag tgcggcgatg tcgaacgaaa gtgtggtcac
     4981 cgcgagcagc gcgtcccgct cgctgatcgg caggagtttg cgcattgact cgagaaggtt
     5041 cgtgagcgcg ccgtgagtaa cctgaacccc tttgggccgg ccggtctacc ccgaggtgta
     5101 gatgacatag gcaaggttgg ccgcggccgg ttcgccatcg agattctcat cgctctgggc
     5161 gtcaaccgcc gaccgctcag cgtcgaggca aagtacgcgc gcctcgcagt gtggcaggcc
     5221 gccgcgcagt ttttcctcgg tgaggaggat cgaggcgccc gcatcctcga gcatgaacgc
     5281 gagccgttgg gcgggatagg ccgggtcaag tggaacatac gcaccgcctg ccttgaggac
     5341 ggccaagagg cccaccacca tgtgagccga ccgttgggcc gccacgccca ccagtacctc
     5401 gggtccgacg cccatagcgc ggaggcggcg agccagacgg ttggcccggg cgttgatctc
     5461 gaaatatgtg agcgactcac cgccgcagaa gagggcaatg gcgtctggag ccagttgcgc
     5521 gcgcatttcg atccgacgat ggacgagcgt cggtccgtct gggcgatgcc cggctttctc
     5581 ctgcagcagc cgcgctacta attggcgctt ctgctccagg ctcggcgcga cactctcttt
     5641 gacatgactc atcgttcgat cctcatttct cagcggagtc gatcgatcag ggcgtcgagg
     5701 gccgactcgg tgaactggtc gaggtgcagg tcgtccggca agggttcact ctccgaccgg
     5761 ttccactgcc cgatcagttg ctcgtgctcg ttcggtgtca tcaagggcag atcgacgagt
     5821 cgctgttcgg gctgggcggc catcgcctcg agtacggtac gaaggtgccc gagcgtctgc
     5881 tcgatggcgg agggatcgaa tcggtcgctg tcgaaaccga tcttgatccc cagctcggtg
     5941 cccggcacca cggtgacggc gagtgggtaa tgggtccgct ccaggtatcg cgcggactcg
     6001 atccccagcc ggtctgcgcg ttcttgcagg gaggctacgt aggggaggtt ctggacgatc
     6061 acgatgctct cgaacagcgg aatgccggcc ggcacttcgc tccaagactg gatttgcgac
     6121 agcggaatcg cctcgaatcg ccgcagctcg atcatggttg actgaagctc gtgcaaccac
     6181 gggatgagga aagcctcctc gatcacctgg acgcggagcg gaagggagtt gatgaacatc
     6241 ccgaccatca gctccactcc ggatagctca gggggccgac cggacaccgt cacgccgaac
     6301 aggacgtcac tacggccgct gtacctggaa agcagtaggg cccacgcccc ttggatcaga
     6361 gtgctcaaag tgagccggtt cgatcgcccg acggcttgga gcctggccgt cacgtccgcg
     6421 ggcagcacgg tctcacgctc gacggcgcac tcccgcaacg tgccgcgccg tcccggcgag
     6481 catccgtcta acccaagttg tgtcgccgag gtgaagcctc tcaagacttg cctccagtat
     6541 ccctccgcct tttcgtcgtc ccgattgcgc agccaggcga cgtagtcgcg aaacggccgc
     6601 atcggttgca aagcgggctc ccgctctagg cgaatcgcct cgtcaatgtc gagaaactcg
     6661 tggagcaata tcgagaggca ccagccgtcg atcacggcat gatgtatgct ccatatgcac
     6721 tcatgtgcag tctcactgag ccggaaaacg gccagccgcg ataggggagg cttggaggga
     6781 atgaatccgc gccgacggtc cgagccgaga taggctgtga gacgctcttc ctgctcggcg
     6841 ggcgtaagac ctcgccagtc ctgatattcc aaaggctggt cggcccgacg atggacgacc
     6901 tggtaaggcc gatcgaaatc gatccagtgg atcgtggacc gaagcgcggg gtggcgcgcg
     6961 accaggcgat tccacgactc ctgaagggca gccgggcgga gctcgccctg aatccgacag
     7021 acaaattgct cgacgtagtg tccagcctcc gggtcggcga gcgtatggaa aagcatgcct
     7081 tcctgcatcg gcgtgagcgg atacaggtcc tcgatgcctc cgtcatccgc gaatgcgcgc
     7141 ttcactgcct cgaggtcgat gccgtcgggt gtcaactcga acggagcgat cgcaggagtt
     7201 gaaatccgac tcgagccctg gtgatccgag ctcgactcga ccgccgccgc gaggtcggcg
     7261 atgttcgggt gccggaacag gtgggctggg ccgaaggcca gtcccgcttg ccgcgctcga
     7321 gacaccatct ggatgccaag gatcgagtcg aatccgatct cgaagaaatt gtcgtggata
     7381 ccgacgcggc tccgccccag caaatccgcg gcgatgccgg ccaagatctc ctccgccccc
     7441 gtgcgcgggg ggacgtacgc ggtcgccgag ccgtccactt caagggcggc cagaggcgga
     7501 agggccgatc ggtcaagctt gccgttgggc gagagcggca acgaatcgag gaaaacatac
     7561 gatgacggga ccatcggctc tggaatacgc tccttcagcc agcgcctcag gtccatcgcc
     7621 gtcgggacgg aaagagctgc gggcaccaca tacgctgcga gtcgcttgtc tcccagggca
     7681 tccgcgacgt ccacgacaac agtctcgctc acgctcgggt ggcgcgccag cacggcctcc
     7741 acctctgcga gctccactcg gaagccccga actttcactt gcccgtctcg ccgacccagc
     7801 agctccagca ctcctccgtc tcgccagcgc gcgcggtcgc ctgtcgcgta cattcgcgtt
     7861 ccgcgccctc cgtgcggatc cggtacaaac cgctcggccg tctgacgcgg attggagagg
     7921 tatccgcgtg ccacacccga gccaacgatg tacagctcgc cggccacacc gaccggtacc
     7981 ggctcgcccc gcctgtccag gacgtaggtg cgcgtgccgg gaaatggacg gccgatcggg
     8041 actgggccgt cctcgccttc gaggccttgc ggcggaccgc taaagtaggt gctgtcgatc
     8101 gtcgcctcgg tcaggccgta ggagttgacc acccgggcgc cgggcccgac cagcgcgccg
     8161 agccggcggt atagacgtcc gcgcacggtg tcggagccga cggccagaag ccgaacacct
     8221 cgcaagtctt cgccctgaag ctctaagtgt gtcgccagag cgtcggcgag cgccgggacg
     8281 agttcgaggc actcgatacg ttcgcgttgg atgagattgg atagcgcagc cgggtcgagc
     8341 aataccgtgc gagggcaagc gacgagagtt ccaccggtgg tcagcgcgcg gcaccagtca
     8401 ccggtgaaga cgtcgaagcc aaaaccggcg gcctgaagat gccgaagcgg aggctggcac
     8461 aggtcgtagg cgtgctccca ggcggctgtg atggcgagca ggctgcggtg cgaaaccatg
     8521 actcccttgg gccgcccggt gctgccggag gtaaagacga cataggcaag gttttcaggg
     8581 tcgacgggca ctcgagggtt ctccggctca actcccgcga gcgactcacc ggaggattca
     8641 agagtaacga ccaggggagt gcataccgga atccgaccgc gcaaatggtc ctcggtcacc
     8701 agtacggtcg ccccggaatc ttcaagcatc gcccctagtc gctcggcagg atggtcggta
     8761 tccagcggca aataggcccc gcccgccttc aaaacgccca ggagaccgac cattctcgac
     8821 gtccaccggt cgaggtagag tcccacgacg gtctccggcc gcacaccgac tccaagcagg
     8881 cgatcggcca gcaaattggc taaccggttt aaatcgcggt atgtgagaga ttgctcactc
     8941 gacaccagcg cgaccgcgtc gggtgagagc tcgacttgtc tctcaaagcg atgatggatg
     9001 gcgatgtcct cgtagggaat cgccggcgcg tcggcccagt cgcccagcaa ttgatggcgc
     9061 tctgagccgg agaacagggc aagatccgct aacctgcgac cgggatcggc gacgatggat
     9121 tccagcaggt ttcggaaccc gtccgccatg cggtcgatcg tcgacgactt gaagaggtcg
     9181 gtgctgtact cgagcgccac acacagccgg tcgccatccc tggcggtcat catcgtcagg
     9241 tcgaagagcg cagtctgcct ttcgaatgca atcgactcga cggcgagtcc gtgcagatcc
     9301 agcctggctc cgggaacgcc cagcgcgaag ggggccaggc cctggtcgtc gaggggctgg
     9361 atcttctggt gggcgaacat gacctgaaac aagggcggcc ggctgggatc tgggttcccc
     9421 tggagtcgat ggacgaggag accgaagggg aagtcttgat gctccagccc gtcggatacg
     9481 gtgcgacgga cgcggcccag gtattcgtca aaagtggggt tccccgagag gtcgcccctc
     9541 atcggtatga ggttgacgaa atagccgacc agatcctgca agccggggag cgttctcccg
     9601 gccaccgggg tgccgacgac aatgtcggcc tggccgctga aacgcgccag caatgcctga
     9661 aaggccgcca gcaggaccgt atacagactc gccccgcgcg actcgcccag ggcgatgatc
     9721 gcccgggtca gcgtcggatc aagataatag tgtttgatcg cgcccttgta gctctggatg
     9781 ggcggcctcg cgaagtcggt cgaaaggtcg agcacgggga gcggctgcgc aagctggtcc
     9841 cgccagtact tccaatgccg ctcgccttcc tcgccaacga ccatctcatg ctgccagcgc
     9901 gcgaattgtg cgtagctcga cactggcgga ggaagctcac cgtcctgtcc tgcacagagg
     9961 gcggtgtatg cggttttgaa gttgtcaagg aatacggccg tggaccagaa gtcggcgatg
    10021 atgtgatgga agaccaagag gaacacgtgc tccgaggcgg accggctgag aagatggacc
    10081 ctgaaaaggg ggcccctctc caggtcgaag gggcggcgag ccagctcggc cagcctctcg
    10141 tggagcgcgc catcgtcaca gcccgggacg tcctcgaccg ggagccattc gtcctcgcgg
    10201 agggcgagat cggcaagatc gaggaggcgg atcgcgggct tttcgtcgat aaggacgaaa
    10261 gtggtccgca gtgcttcctg gcggacgatg acgcggcgca tcgcctgccg gaaggcgctc
    10321 ttgtcgagct ctgcgcgcac ctttccggcg ccgaggacgt ggtaggcggc gccggtcgtc
    10381 gtgaactggt gagcatacca gagcatctgc tgcccgtgcg aaagcggtgg gccggtc
//
LOCUS       DATAQL010000796         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1302772, whole genome shotgun sequence.
ACCESSION   DATAQL010000796 DATAQL010000000
VERSION     DATAQL010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302772"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VHS97_13895"
     CDS             <1..1081
                     /locus_tag="VHS97_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449347.1"
                     /translation="VGAEDGQLTSEQCRLLKGLVEHQASGLVFMPGWQGRELSLLDTE
                     LGDLCPVVLDTAQPGGWGSRVANHFELTELGRRSLLTKLADTQDDNAEVWEGLPGFQW
                     YAPVLRAKAGSDVLCVHKDASNEYGRLPLLVTRTFGAGKVLFMGTDGAWRWRKGVEDK
                     YHYRFWGQVVRWMAYQRNMAKGETMRLYYVPDQPKLNQTLALHANVMEQGGEPLHGGE
                     VTARIIAPSGKADLIRFTSAGDEWGEFTGRFSALESGSHRVTLFCKQTGATLEASFFV
                     QGAAAEQVGRPARPEVMEELARVTGGKVIEAGKLDEVVRSLAQLPDPAPLVRRVPLWC
                     HPVTALALIGLMGVFWVGRKVVGLV"
     gene            1130..>3447
                     /locus_tag="VHS97_13900"
     CDS             1130..>3447
                     /locus_tag="VHS97_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449348.1"
                     /translation="MSLVYTPDRLQLPDTLQAQLHEFRRRVWMIKTVEAVAAATFGVL
                     VAYLVLFCLDRAWDTPGWLRVSLFVTAAVACANIPWALHRWVWRHRRLEQLARLLSRK
                     HPRVGDQLLGIIELVRNDFEQARSRALCEAAIREVAHDAGRRDFRDAVPNPRHRLWTW
                     LVAMPTVTALVLFALFPAAAANAWQRFLVPWKNAPRYTFTMLERLPEKIVVAHGEPFT
                     VALQLTDQTVWHPKDGVARLGVQTPVVAPLRDGRYKFELPPQIDAGWLNIKIGDTISR
                     VRIEPTLRPELVSVIASVALPGYLGRTELERKDVRGGAVSLVLGSTATFTATASRELK
                     NAYVDGQSLEPAGAALSSPPAKVDGSRKIEFRWQDQNGLAGKEPFVLAITGREDEAPS
                     LSCEDLPRQKVVLDTELLSFKVRAQDDFGIKEIGLDWQGVDSPLVSTPAKGERILSAG
                     GQHSDTLEINGTFSARSLSIDPQPVQVRLFATDYFPGRPRVYSPTYTFYVLNAEQHAI
                     WVTEQLSKWHRQSLEVRDKELQLFDTNKQLRALTAEELDRPDTRRKIENQASAERANG
                     RRLSALTGTGEDLIKQAMRNPEFNIASLEKWAEMLQILKDIAGNRMPSVADLLKQAAQ
                     APSTAMSNSKNTSKMAGQNRAGGSTSPSLPPDENKKPPTAVPQVVDRESSQQPADPKA
                     GAQASKSSNKTPRLTLPATTLDGKAGPSKPKEAAAEERVDEAVVQQQDLLAEFEKIAD
                     ELNRVLANLEGSTLVKRLKASSRLQSKVGGRI"
BASE COUNT          639 a         1095 c         1108 g          605 t
ORIGIN      
        1 cgtaggggcg gaagacggcc agttaacttc cgagcagtgc cggctgttga aggggttggt
       61 agagcaccag gcgagcggtc tggtgttcat gccgggctgg caaggtcgcg aattatcctt
      121 gctagatacc gagctaggag acctctgccc ggtcgtgctc gacacggccc agcccggcgg
      181 ctggggctcg cgggtggcca accattttga actaaccgag ctgggccggc gcagcctgct
      241 cacgaagctg gccgacaccc aagacgacaa cgccgaggtt tgggaggggc tgccgggctt
      301 tcagtggtat gcccccgtgc tccgggccaa ggccggcagc gacgtcctgt gcgttcacaa
      361 ggacgcgtcc aacgaatacg gccgtctccc gctcttggtc acgcgcacct tcggtgccgg
      421 caaggttctc ttcatgggga ccgacggagc ctggcggtgg cgcaaggggg tggaagacaa
      481 ataccattac cggttttggg gccaggtggt gcgctggatg gcctatcagc ggaatatggc
      541 caagggggaa acgatgcggc tgtattacgt ccccgatcag cccaagctga accagacact
      601 tgcgctgcac gccaacgtga tggagcaagg tggcgagccg cttcacggcg gcgaggtgac
      661 cgcacggatc atcgccccat cgggcaaggc cgacttgata cgcttcactt ccgccggcga
      721 cgagtggggc gaattcaccg gacgctttag cgcactcgaa tcgggcagtc accgggtcac
      781 actattctgc aagcagaccg gcgcgacctt ggaggcctcg tttttcgtgc agggggcggc
      841 ggccgagcaa gtgggccggc cggctcggcc ggaggtgatg gaagagctcg cccgggtgac
      901 cggaggcaag gtcatcgagg ccggcaagct cgatgaggtt gtgcggtcgt tggcccagct
      961 tcccgacccg gcacccttgg tgcgccgggt gcctctctgg tgccaccccg tgaccgcgct
     1021 ggctttgatc ggactcatgg gagtcttttg ggtaggacgt aaagtggtcg gtcttgtata
     1081 atcgcccgac ggacgtcatc gacgcttcgc actttcttgt gaggtcaaca tgagcctcgt
     1141 ctatacacct gatcggcttc agttgcctga cacgcttcag gcgcaacttc acgagtttcg
     1201 ccgccgggtt tggatgatca agacggtcga agcggttgcc gcggcaacct tcggcgtcct
     1261 ggttgcctac ctcgtgctgt tttgcctgga tcgcgcgtgg gacacgccgg gttggctccg
     1321 cgtcagcctg ttcgtgacgg ccgcggtcgc gtgcgccaac atcccctggg ccttgcaccg
     1381 ctgggtctgg cgccatcgtc ggctcgaaca actggctcgc ctcttgtcgc gcaagcaccc
     1441 ccgggtcggc gaccagttgc tgggcatcat cgagctcgtg cgcaacgact ttgaacaggc
     1501 ccggtcacgc gcgctttgcg aggcggcgat ccgcgaggtc gcccacgacg cggggcgacg
     1561 tgatttccgc gacgccgtac ccaacccgcg acatcggctc tggacctggc tagtcgcaat
     1621 gccgacggtg acagcgctcg tgcttttcgc ccttttcccg gccgccgccg ccaacgcctg
     1681 gcagcgattt ttggtgccgt ggaaaaacgc cccgcggtac acgttcacga tgctcgagcg
     1741 ccttcccgag aaaatcgtgg ttgcccatgg cgagcctttc accgtcgctc tccagctcac
     1801 cgaccagacc gtctggcacc cgaaagacgg ggtcgcgcgg ctgggagtac agacgcccgt
     1861 ggtcgcgccc ttgcgcgatg gccgatacaa attcgagctg ccgccgcaga tcgatgccgg
     1921 ttggctgaac atcaagatcg gcgacaccat cagtcgcgtg cgcatcgagc cgaccctgcg
     1981 cccggagctg gtctcggtga tcgcctcggt cgcgctgccg ggttatctgg gtcgtacgga
     2041 gctcgagcgc aaggacgtgc gcgggggtgc ggtctcgctg gtcctgggaa gcacagcgac
     2101 ttttaccgcc accgccagcc gcgagctaaa aaacgcctat gtcgacggtc agtcgctcga
     2161 gcccgcgggc gcggctctgt ccagcccgcc ggcaaaagtc gatggctcac gtaagatcga
     2221 gttccggtgg caagaccaaa acggtctggc cggcaaagag ccgttcgttc tggcgatcac
     2281 cggccgcgag gacgaggcac cgtctctttc gtgcgaagac ctgccacggc agaaggtcgt
     2341 gctcgatacc gagctcttga gcttcaaggt tcgtgcccag gacgatttcg gcatcaagga
     2401 gatcgggctc gactggcaag gcgtcgacag ccccttggta tcgacgccgg ccaaggggga
     2461 gcgcattctc tcggcaggtg ggcaacattc ggacacgctc gagatcaacg ggacgttttc
     2521 ggccagatcg ctgtcgatcg acccgcagcc ggtgcaggtc cggctattcg cgaccgacta
     2581 ctttccgggc cgcccgcgcg tctattcgcc aacctacacg ttctatgtct tgaacgccga
     2641 acagcacgcg atctgggtca ccgaacagtt gagcaagtgg caccgccagt ctctcgaggt
     2701 gcgcgacaaa gagctgcagc ttttcgatac caacaagcag cttcgggccc tgacagccga
     2761 agagctcgac cggcccgaca cccgacgcaa gatcgaaaac caggcatccg ccgagcgcgc
     2821 caacggccgc cggctctcgg ccttgaccgg caccggcgaa gacttgatca agcaggcgat
     2881 gcgtaaccca gagtttaaca tcgccagcct cgaaaagtgg gctgaaatgc tccagatatt
     2941 gaaagacatc gccggtaatc ggatgccgtc ggtcgccgac cttttgaagc aagcggccca
     3001 ggcccccagc acggcgatgt ctaattccaa gaacacgtcg aagatggccg gccaaaaccg
     3061 ggccggagga tcgacatcgc cgtccctgcc gccagacgag aacaagaagc ctccgacggc
     3121 cgttccgcag gttgtcgatc gcgaatcatc ccagcagcct gccgatccca aggccggcgc
     3181 tcaggcatcg aagagcagca ataagacccc ccgtctcacc ttgcccgcga ccaccctgga
     3241 cggcaaggct ggtccaagca agcccaagga agcggctgcc gaagaacggg tcgacgaggc
     3301 ggtcgttcag cagcaagact tgttggcgga gtttgagaag atcgccgacg agcttaaccg
     3361 tgttctcgcc aacctcgagg ggagcacgct ggtcaagcgg ctcaaggcat cgtcgcggct
     3421 ccagtccaag gtcggcggcc ggatcag
//
LOCUS       DATAQL010000797         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1150587, whole genome shotgun sequence.
ACCESSION   DATAQL010000797 DATAQL010000000
VERSION     DATAQL010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150587"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(740..2389)
                     /locus_tag="VHS97_13905"
     CDS             complement(740..2389)
                     /locus_tag="VHS97_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3449349.1"
                     /translation="MPEHDLESVALPTLDAAQLAALERCPLTKLQRYKDGEKLYEAGD
                     RDFRLFIVKSGALEIVDESGEQPKAVTELKPGQFTGDVTQLTGGPALVSAVARGDCEV
                     YEVSPDALQQLLNNHPDLGDLILRAFIARRQLLKELGTFKGLIVIGSRHSPGTFRVRE
                     FLAKNGVLSTWLDLEEDPQVQQVLQRFRVSEAESPVVAWGSKLLLRNPSNRQLAEVLG
                     LRRPVERKVYDLLVVGAGPAGLAAAVYGASEGLSTVVLERSGPGGQAGRSMRIENYLG
                     FPTGITGAELAERAWVQARKFGACLAVGTQVMRLTFDDGRPLIEIDGGETVSARCLLI
                     ATGAEYRRLDVDGCEQFEGCGVYYAATAIEALMCQGSEVVVVGGGNSAGQAAVFLAGQ
                     VRKVYLVIRGHDLYRDMSYYLARRIEATPNIEIVLNSTFRRMCGDGYLESVELVNTKT
                     REVRTLKTPAVFSFIGALPRTDWLPPEIERDAKGFVRSGRALAQCPHWTAERQPYFLE
                     TSHPGVFAAGDVRSGSVKRVASAVGEGSMAVQFVHACLKEL"
     gene            complement(2480..3727)
                     /locus_tag="VHS97_13910"
     CDS             complement(2480..3727)
                     /locus_tag="VHS97_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3449350.1"
                     /translation="MNWSWRIGRIAGIDVYVHFTFVLLLGWVALSHYMANGDLAEAMG
                     GLIFILALFGIVVLHELGHALTARHYGIRTRDITLLPIGGVARLERMPEEPRQELVVA
                     LAGPAVNVALALGIYLILALGQGLAPVGDVLQVGGGLLSQLFWVNVSLAVFNLLPAFP
                     MDGGRVLRAFLAMRTDYVRATQTAASIGQGMALLFAFLGLFSNPFLIFIALFVWLAAT
                     QEASLVQMRSALDGIPVQRAMIRYFRTLSADEPLSRAADYVTAGFHHDFPVVEDGRLV
                     GMLTRPALATALAQFGPQGRVRDAIQRDIVTVAPAEMLQTALAKLQDCDCRTLPVVQD
                     GRLMGLVTADSLAEVLMIQEALGEARRLGRSPAQAQGRDGAGVALRLSDDTAFDELAR
                     PKTASLGAGDAINPGAGRTPRGG"
     gene            complement(3791..>4339)
                     /locus_tag="VHS97_13915"
     CDS             complement(3791..>4339)
                     /locus_tag="VHS97_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449351.1"
                     /translation="ARAIDRHKGKVTVFVPHYAMSGGTLIALAADEVIMSDHAVLGPV
                     DPQLGQSPAASLLRVIKTKPIAEVDDQTLIMADQAEKAVVQLRECVKALLSPKVGHER
                     AAELAAILTEGTWTHDYPITFDMARELQLPVSSAIPEEVMQLMSLYPQPVRRQASVEY
                     LSRPRHLEPRGALPAGSGVNQP"
BASE COUNT          802 a         1450 c         1302 g          785 t
ORIGIN      
        1 ctttagaaac cctggaaatc aagatcgcag gacagatcac acttaactcg ttactattta
       61 atgctttgcg tctgagtaga ccgcctgttt tgcaaccagg aggttgccgg ttcgatcccg
      121 gtcgtctcca ctgaacatta atccggagtt cgagttacgt ccctgaatcg ctgcggcgaa
      181 ttaccgcgca ggggtgagca tgcaattgag caaggcgcgg aattgtccct tgattctgcg
      241 ggtttttcat ttgcgcgagc gcctggtcaa ataccaggcg ctcctatcac cttcaaatcg
      301 tcaaactggg tgattctcaa atctatgtcg gcttgcgtca ccgcaattca tcttcgaaaa
      361 catcgccggt taagaattat gcatggtgtc cccggaactc cgaatttcaa agcacttaag
      421 cccgacttcc atgccagtcc cacgctaccc ctacggcaga caatgaaaac ttaaaatcct
      481 ctcctggggc acctgggtgt gggttcgatt ccccccgccg gtactctgat tgatcccgat
      541 atgacgcctc agatgccttg ttttggaggg ttgaatcgat cttgaccatc ggagcatcgg
      601 aatttgtatt cacgtcatca cccaaattat tcagtccctt cccccccact cgggtcggca
      661 aatggtgggc aagtccagct tcgaaagcct tcgccgttcg acccggagca catcggcgac
      721 tcgacgctgg cccttgcctt tacagctctt tcaggcacgc gtgcacgaac tgcaccgcca
      781 tcgacccttc cccgacggcg gaagcgacgc gcttcacaga gccagagcgc acgtcgccgg
      841 ccgcgaacac gccggggtga ctcgtttcga gaaaatacgg ctgccgttcg gcggtccagt
      901 gaggacactg ggccagagcc ctccccgagc ggacgaatcc ctttgcatct cgctcgattt
      961 ctggcggaag ccaatcggtc cgcggcaggg caccgatgaa gctgaaaacc gccggggtct
     1021 tcagcgtccg aacctctcgg gtcttggtgt tgacgagctc gactgactca agatagccgt
     1081 cgccgcacat ccgccggaaa gtcgagttaa gaacgatttc gatattggga gtcgcttcga
     1141 tccgccgggc caggtagtat gacatgtcgc ggtacaggtc gtggccgcga atgacgaggt
     1201 agactttgcg aacttgcccc gcgaggaata cggccgcctg gccggcggag ttgccgccac
     1261 cgacgaccac gacctcggac ccctggcaca tgagcgcctc aatggcggtc gcggcgtagt
     1321 agaccccgca gccctcgaac tgctcgcacc cgtccacgtc cagcctgcgg tattcggccc
     1381 cggtggcaat gagcaggcac ctggccgaca ccgtctcgcc accgtcgatt tcgatgagcg
     1441 ggcggccatc gtcgaaggtc aggcgcatga cctgcgtgcc tacggccagg cacgcaccga
     1501 atttcctggc ctggacccac gcgcgctctg ctagctcggc gccggtgatg ccggtgggga
     1561 aaccgaggta gttttcgatg cgcatgctgc ggcccgcctg acccccgggc cccgagcgct
     1621 ccagcaccac cgtgctcaag ccctccgagg cgccatagac cgccgccgcc agacccgccg
     1681 ggccggcccc caccaccagt aaatcgtaca ccttccgctc caccggccgg cgcaggccca
     1741 gcacctccgc gagctggcgg ttcgaggggt tccgcagcag cagcttggaa ccccaggcga
     1801 caacgggcga ctcggcttcg ctcacccgaa accgctggag cacttgctgc acctgcgggt
     1861 cctcttcaag gtccagccag gtggacaaca ctccgttctt ggccaggaat tcgcgcactc
     1921 ggaatgtgcc cggcgaatga cgcgaaccga tcacgatcaa gcccttgaag gtccccagct
     1981 ccttcaggag ctgccgccgc gcgatgaacg cacgcaggat gaggtcgccc aggtccggat
     2041 ggttgttcaa gagctgctga agcgcgtcgg gagagacttc gtagacttca caatccccgc
     2101 gcgcgacggc gctgacgagc gccgggccac cggtcagttg cgtcacgtcc ccggtaaact
     2161 gcccgggctt gagctccgta actgccttgg gttgctctcc tgattcgtcc acaatctcga
     2221 gcgcgccgga cttgacgatg aagagcctga agtcgcggtc gccggcttca taaagcttct
     2281 cgccatcctt gtagcgctgg agcttcgtca agggacatcg ctccagcgca gccaactgcg
     2341 ccgcatcgag cgtgggcaag gcaacgcttt ccaggtcgtg ctcgggcatg cctcgaatcc
     2401 cccccgtttc gctcatcagg ttcacctcag gtacggctcg acctcctcgg cgacctcgag
     2461 agtcgtctga tcgactttgt cagccacctc gcggcgttct ccctgcaccc ggattgatcg
     2521 catcgcccgc gcccaacgag gccgtcttgg gcctggcgag ctcgtcgaag gcggtgtcat
     2581 cggaaagcct caaggcgacg ccagccccgt cgcggccctg ggcttgggcg gggctccgac
     2641 ccaggcggcg cgcttcgccc aatgcctctt ggatcatcaa cacctctgcc aggctgtcgg
     2701 ccgtcacgag acccatcagc cggccgtctt ggacgaccgg cagcgtgcgg cagtcgcagt
     2761 cctgaagctt ggccagggcg gtttgcagca tttccgctgg cgcgacggtg acgatgtcgc
     2821 gctggatggc atctcgaacc cggccctgtg gaccgaattg cgccagcgcg gtcgccagcg
     2881 ccgggcgcgt gagcatgccg accagccgcc cgtcctcgac caccgggaaa tcgtggtgga
     2941 aaccggccgt gacgtagtcc gcggcccgcg acaagggctc atccgcgctc aaggtgcgga
     3001 aataccggat catcgcgcgc tggacgggga tgccgtccag tgccgaccgc atctgcacca
     3061 agctggcctc ctgggtcgcg gccagccaga cgaaaagtgc aatgaagatc aggaaggggt
     3121 tggagaaaag gcccaggaag gcgaacaaga gcgccatccc ctggccgatc gaggctgccg
     3181 tctgcgtggc acggacgtaa tcggttcgca ttgccaaaaa cgcccggagc acccggccgc
     3241 cgtccatcgg gaacgccggc agcaagttga acacggccag cgagacgttg acccagaaga
     3301 gctggctcag gagaccgccg ccgacctgta gcacgtcccc taccggtgcc agcccctggc
     3361 ccaacgccag gatgagataa atgccgaggg ccagtgcgac gttgacggct ggcccggcca
     3421 gcgccaccac cagctcttgg cgaggctcct cgggcatccg ctccaatcgg gccactccgc
     3481 cgatcggcag cagggtaatg tcgcgcgtgc ggatgccgta gtgccgggcg gtcagcgcgt
     3541 gacccaactc gtgcaggacc acgatcccga acaatgccag gataaagatc aggcccccca
     3601 tcgcctccgc caggtcgccg ttggccatgt agtgtgacag ggccacccat cccagcagaa
     3661 ggacaaacgt gaaatggacg tagacgtcga taccggcaat tcggccaatc cgccaggacc
     3721 agttcatggc tgctctccgg attctccaca cggttgtctt gtggttgcgc ttgggcctga
     3781 agctgccgcc tcagggctgg ttcacgccgc ttcccgcggg cagggctccg cggggctcga
     3841 ggtggcgtgg tctcgacaga tactccacgc tcgcctggcg acggaccggc tgcgggtaca
     3901 gactcatgag ctgcatgacc tcttcgggaa tagccgagct gaccggcagc tgcagttccc
     3961 gtgccatgtc gaacgtgatc ggatagtcgt gcgtccaggt gccctccgtc aggatcgctg
     4021 ccagctctgc cgcccgctcg tggcccactt tcggcgagag cagcgccttg acgcactccc
     4081 gtagctgcac gaccgccttc tcggcctggt cggccatgat caacgtctgg tcgtcgacct
     4141 cagcgatcgg cttggtcttg atcaccctga gcaacgaggc ggcgggagat tggccgagct
     4201 gaggatccac cgggccgagc acagcatggt cgctcatgat gacttcgtcg gccgccagcg
     4261 cgatcagcgt ccccccggac atcgcgtagt gagggacgaa gacggtcacc ttgcctttgt
     4321 gccggtcgat ggcccgcgc
//
LOCUS       DATAQL010000798         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_693410, whole genome shotgun sequence.
ACCESSION   DATAQL010000798 DATAQL010000000
VERSION     DATAQL010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693410"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VHS97_13920"
     CDS             complement(<1..470)
                     /locus_tag="VHS97_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449352.1"
                     /translation="MRRRDFLPALATASVAAAQTTNPQAPRKGRLKLACFRRNFDPKM
                     PFDEMCRQAARIGFQGYSGASMQDWPTLKTYGLTPSMSVGGGVTIDNGIIRKELHEDQ
                     VKSLGTFIDACSAAGGSDILITGGRRHGMPYNEGADMAVNFLNRVKNRAEDKGI"
     gene            540..1856
                     /locus_tag="VHS97_13925"
     CDS             540..1856
                     /locus_tag="VHS97_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449353.1"
                     /translation="MLLMLAAGIAIGLTISNRMARVEAQSAPGTGYAAVPADLGSEDL
                     TGPYEVVKNWPQDISTLPGNAKWTYGAGESVFAESPNRIYMLFRGELPKMAPPKPVLL
                     PQLGPSISFPVAGFWRDATTASLPGTGGTDNDMSEWLTAWEGKGKKEGMKGPPYRQLG
                     VDAKWENCLVVVDGSGKIIETWSQWDKLFRRPHSVYVSPYDPEKNVWVVDDNMQVIYK
                     FSHDGKKLLQTIGTPEVAGADATHFNRPTYIDWLPDGTFFVSDGYTGTRVAKFNKDGK
                     FLMDWGKLGGPPRGPVDTRPGYFSNVHGVAVDPATRHVFVNDRNNHRIQVFDENGKYL
                     YEWKISADPSSLHLLYIGSGKSIWTYDRTTNKMVEWDLQGHLLYSWGSMGNFPGGLWG
                     VHGMSVDQEGNLYVAEVDSGRVQKFRPRAGANPAFLVAKPVYSAWK"
     gene            complement(1905..>2261)
                     /locus_tag="VHS97_13930"
     CDS             complement(1905..>2261)
                     /locus_tag="VHS97_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449354.1"
                     /translation="NVVLIEMIETVNGVKEAREIAGIKGVDAVFAASGDLGNFSGYKQ
                     GSPDYERLINAVHDAAIAAGKRLCGPFAWRDRPDFTCFQAGSETAAIARGVAAELGPL
                     KDTQGKPEVGPYAPKK"
BASE COUNT          455 a          628 c          728 g          450 t
ORIGIN      
        1 ttgatgcctt tatcttctgc acggtttttg acgcggttga ggaaattgac cgccatgtcg
       61 gcgccttcgt tgtaaggcat gccgtgccgg cgtccaccgg tgatcaggat gtcggagcca
      121 cccgcggcgg agcaggcgtc aataaatgtc ccgagcgatt tcacctggtc ttcgtgcagc
      181 tccttgcgga taatgccgtt gtcgatggtc acgccgccgc ccacgctcat ggaaggagtc
      241 aggccgtacg ttttgagcgt cggccaatcc tgcatcgacg cgccgctgta gccctggaat
      301 ccgatccggg cggcttgacg gcacatctcg tcgaatggca ttttgggatc gaagttccgg
      361 cggaaacacg ctaacttcaa tcgacccttg cgcggggctt ggggattggt ggtctgcgcg
      421 gccgcgacgg atgcggtggc cagcgccgga aggaagtctc tgcgtcgcat gggtgaatga
      481 tatcgaaaag cgagagcccg aagcggcgta tactgtcact tatgcgaaaa gtttggctaa
      541 tgctattgat gctggccgcc ggtattgcga tcggactgac gatttcgaac cggatggcgc
      601 gagtggaagc acagtcggct ccagggaccg gttacgcggc cgtgccggcc gacttgggat
      661 ccgaggacct gaccgggcct tatgaagtgg tgaagaactg gccgcaggac atcagcacgc
      721 tgccagggaa cgcgaagtgg acctacggcg cgggcgagag cgtcttcgca gaaagtccga
      781 atcgcatcta catgctcttc cgcggcgaac tgccgaagat ggcgccgccg aagccggtgc
      841 tgctgccgca gctagggccc agcatcagtt ttccagtggc tggattttgg cgcgatgcta
      901 cgacagcttc tcttcccggc acgggcggca ccgacaacga catgagcgag tggctgactg
      961 cctgggaagg caagggaaaa aaggaaggga tgaagggccc gccctatcgt cagctcggcg
     1021 tcgatgcgaa atgggagaac tgcctggtcg tcgtggacgg aagcgggaag atcattgaaa
     1081 cgtggagtca atgggacaag cttttccggc gccctcactc ggtttatgta agcccgtacg
     1141 atccggaaaa gaacgtatgg gtggtagacg acaacatgca ggtcatctac aagttcagcc
     1201 acgatggcaa gaagctgctg cagaccatcg ggacgccgga agtagccggc gcggatgcga
     1261 cgcatttcaa ccgccccaca tatatcgatt ggctgcccga cggcaccttc ttcgtgtcgg
     1321 acggttacac cggcacgcgc gtggcgaaat tcaataagga cgggaagttc ctgatggact
     1381 ggggaaagct aggcggcccg ccgcgcggtc cggtggacac tcgtcctggc tacttcagca
     1441 atgtgcatgg cgtggccgtg gatccggcaa ctcgccacgt tttcgtgaac gatcggaaca
     1501 atcaccgcat ccaggtcttc gatgaaaacg gcaagtatct ttatgagtgg aagatcagtg
     1561 ccgatccatc gagtctgcac ctgctctata tcggatcagg caagtccatc tggacgtatg
     1621 accgcacgac caacaaaatg gtcgagtggg atttgcaagg acatctgctt tactcgtggg
     1681 gtagcatggg caatttcccg ggcggcctgt ggggcgtgca tggcatgagc gtcgatcagg
     1741 aaggaaatct gtacgtggcg gaggtcgact cggggagggt gcagaagttt cgtccgaggg
     1801 ccggcgcgaa tccggcgttt ctagtcgcga agccggtata ctcggcgtgg aaatgagccg
     1861 agccgtcgct taagttattg ctagcggctc ggccgatagg ctatctattt cttcggagcg
     1921 tacggcccga cttcgggctt gccctgggta tccttcaagg gcccaagttc ggcggcgaca
     1981 ccgcgcgcga tggcggccgt ctcactgccg gcctggaagc aggtgaagtc cggacggtcg
     2041 cgccaggcga agggaccgca cagccgcttg ccggccgcga tagccgcgtc gtggacggcg
     2101 ttgatcaggc gctcgtagtc cggcgagcct tgcttgtaac ccgagaaatt acccagatct
     2161 ccgctggccg cgaacaccgc gtcgacgccc ttaatcccag cgatttcgcg cgcttccttt
     2221 acaccattga cggtttcgat catctcgatc agcaccacgt t
//
LOCUS       DATAQL010000799         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4205061, whole genome shotgun sequence.
ACCESSION   DATAQL010000799 DATAQL010000000
VERSION     DATAQL010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4205061"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VHS97_13935"
     CDS             <1..308
                     /locus_tag="VHS97_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449355.1"
                     /translation="TIRLTRSQAAMCIKSKASWMVSLGIVGIEKLRTLNGPAVLKERV
                     QGAEIGIVHKRAECRRIAVREDVTHCLGEAPERVFQQRIATPTATNVTFWLAGVKSV"
     gene            723..1781
                     /locus_tag="VHS97_13940"
     CDS             723..1781
                     /locus_tag="VHS97_13940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3449356.1"
                     /translation="MALHLVLSPGIDLDEFRRQSEEGLGPRHSMAVLADRLDAIVHIP
                     HPERDQPTALDGLRSRLIGSRETWAMARRLANQLGRDDVVFCQSETVGLPLVALLRKR
                     SNRPKLYLFGHNLARRRCHVAARLFRLASRADALVVCCSAQAEFLREQLTIPDSRIHI
                     LLEHVDNRFFSPGPASPDKKRRLIVGVGLEKRDYRTLAQATQDLDVDVRISGFSRYAA
                     VLAKSFPDSMPANMTRRFYSWPELVQLYRDADIVVAPLFPCPYAAGVTTLMEGMCCRR
                     PVIVSRSPGLSDYLDPSDGISVVEPFDSNGWRQAIVQLLEHPEKSGDQADRGFRLGSE
                     RYDFDQAVNRFEKLLQSS"
     gene            complement(1860..>3061)
                     /locus_tag="VHS97_13945"
     CDS             complement(1860..>3061)
                     /locus_tag="VHS97_13945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3449357.1"
                     /translation="FWRRNEHCPTEVTITFSSQFEQFCEDIGAEAYIVGYNSRKEILK
                     DGAFTLEHRPKPMPGARGALYHLAEIFYGLGLLVTAVRFRANVAVIDSGSTYYFVTSL
                     FRLMGIRVIAVLYNTLWPHGFPPTRRVPRAILALDSLFYRHVATAVIGVSPECTRQVA
                     QLTGGRHAPLYEISAQFPREYFAQIPPPPPFDHRPFQIMFIGRVNRSKGVFDILEMAQ
                     KVEASVPGQVRWEICGSGHDLEELRHRHRDMGLGSIVNIRGWTPLSDLLEVYARSQAA
                     IVPTRSSFIAGLEMTAAEAILAGRPLITNPVVPALELLRPACVEARTDDVDSYVEGVL
                     KLIGDREYYESLRRACPELGEQFYDRNLGLTAVLKRAIEPLEDRKLRRRDGVAASYED
                     RVDPVGE"
BASE COUNT          619 a          923 c          926 g          593 t
ORIGIN      
        1 gtacaattcg acttacacgt tcacaggctg cgatgtgcat caaatcaaag gcgagctgga
       61 tggtaagtct gggaattgtc gggatcgaaa aactccgcac cttgaacggg ccggcggttc
      121 tgaaggagcg cgtccaaggc gccgaaatcg ggattgtcca caagcgagcc gagtgtcgac
      181 gtattgcagt tcgcgaggat gtaacgcact gccttggaga ggccccagaa cgagtattcc
      241 agcagaggat cgccacaccg accgcgacga acgtcacatt ctggctcgcc ggtgtcaagt
      301 ccgtctgagg aacacgcgcg aagccgtgaa acaatacccg cgtcgtgttg tcgggtaata
      361 gcgcgagcac gacaagttca tcatcatttg cgaggacgta cggtgacggg gtcactgcga
      421 gcggccagat ttgatcgaag tgcgaaggcc agccggtatt gaacgccgcc cgctcgtcga
      481 gaatgtagga gaatcgcgcg gccggtggtt cgatcgcttc cttttattgg atctgatcgc
      541 agcgagtatt gggtagaagg gcgtaaccct ggccgcggtc atagaggaag acgccgatcg
      601 gaatcgcgtc cagcgcgagc tgtgacgggt acggattgta atcgaaagag ccaaccaaga
      661 gttcaccgta gtggaaggac tgagtatact tggattattc taaaccaacg cggaggcagt
      721 gaatggcttt acaccttgtc ctgagccctg ggattgacct cgatgagttt cgccgccaga
      781 gcgaagaggg gttaggtccg cggcattcga tggctgtgct ggccgaccgg ctcgatgcaa
      841 ttgtgcatat tccgcaccca gagcgagatc agcccaccgc gttagacgga ctgcgatcgc
      901 ggctgatcgg gtctcgcgaa acgtgggcga tggcgcgacg gctggcgaat caactgggcc
      961 gtgacgatgt cgtattctgc cagagtgaga ccgtcggatt gcctttggtc gcgttgctgc
     1021 gaaaaaggtc caaccggccc aagctctacc tttttggaca caaccttgct cgccgccgtt
     1081 gccacgtggc cgcccgcctt ttccgcctcg ccagtcgggc cgatgccctg gtggtctgtt
     1141 gctctgcgca agccgaattt ctccgtgaac aactgaccat tcccgattcc cgcattcaca
     1201 tcctgctgga acacgttgat aaccgcttct ttagtccggg gcccgccagt ccagacaaaa
     1261 agcgtcggct gattgtcggt gtgggattgg aaaagagaga ctaccgcacg ctcgcgcagg
     1321 ccacccaaga tctcgatgtc gacgtccgca tcagtggctt ttcgcgttat gccgcggtcc
     1381 tggccaaatc gttccccgat tcaatgccag ccaacatgac aaggcggttt tactcctggc
     1441 ccgagctcgt gcaactgtac cgtgatgccg acatcgtggt tgcgccccta tttccttgcc
     1501 cctatgccgc gggcgtgacg accttgatgg aaggaatgtg ctgtcgccga ccggtcatcg
     1561 tctcgcgctc gccaggcctg tccgattatc tcgaccccag cgacggcata agcgtcgtcg
     1621 aaccattcga cagcaatgga tggcgccagg ccatcgtcca gttgcttgaa caccccgaga
     1681 agtccgggga tcaggcagac cgtggctttc ggctcggctc ggagcgctac gatttcgacc
     1741 aggccgtcaa ccgcttcgag aaacttctgc agagctcctg agctgacctc cgcgatgaag
     1801 gatcagcggg tgtccgtacc cgcgacaacc caatgaagct cggccggtgg acgagcaagt
     1861 cactcgccga cgggatcgac ccgatcctcg tagctcgcgg ccaccccgtc ccggcgccga
     1921 agttttcggt cctcaagggg ctcgatcgca cgtttgagaa cggccgtaag gcccagattc
     1981 cggtcgtaga attgctcgcc tagctcgggg cacgcccggc ggagactctc gtaatactcg
     2041 cggtcgccga tcagtttgag cacaccctcg acgtaactgt cgacgtcatc ggtccgggct
     2101 tccacgcacg ccggccgaag cagttccaga gcggggacga cggggttggt gatcagtggc
     2161 cggcccgcca ggatcgcctc ggccgcggtc atttctaggc cagcaatgaa gctactccgc
     2221 gtcggcacga tcgccgcttg acttcgcgca tagacctcga gcaagtccga gaggggcgtc
     2281 caaccacgga tgttgacgat tgatccgagc cccatgtcgc gatggcggtg tctcaattcc
     2341 tccaggtcat gtccggagcc gcagatctcc cagcgtactt gccccggtac gctagcctcg
     2401 accttttggg ccatctccag tatgtcgaaa acccccttgc tacgattcac tcggcctatg
     2461 aacatgattt ggaatggacg gtgatcaaac ggagggggag gggggatctg cgcgaaatac
     2521 tcgcgcggga actgggcgct gatctcgtac agcggcgcgt gccggccccc cgtaagctgc
     2581 gccacctggc gtgtgcattc gggcgagacc ccgatcaccg cggtcgccac gtggcggtag
     2641 aacaaggaat ccagcgcgag aatcgcccgg ggcacgcgcc gcgtgggggg gaagccgtgt
     2701 ggccagagag tattgtacaa aaccgcaatg acccggatgc ccatcagccg gaacaggctg
     2761 gtaacgaagt agtaggtaga ccccgagtcg atcaccgcca cgttggcccg gaaccgcacc
     2821 gccgtaacca gcaggcccag accgtagaag atctccgcca ggtgataaag cgctccccgg
     2881 gctcccggca tcggcttggg ccgatgctcc agcgtgaagg ccccgtcctt caggatttcc
     2941 ttccgcgaat tgtagccgac gatgtaggcc tccgccccga tgtcctcgca gaactgctca
     3001 aactggctgg agaacgtgat cgtcacttcg gtaggacaat gttcgttccg cctccagaat
     3061 c
//
LOCUS       DATAQL010000800         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4205150, whole genome shotgun sequence.
ACCESSION   DATAQL010000800 DATAQL010000000
VERSION     DATAQL010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4205150"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VHS97_13950"
     CDS             complement(<1..1545)
                     /locus_tag="VHS97_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1595 domain-containing protein"
                     /protein_id="HEX3449358.1"
                     /translation="MNRWGTRTLGGISVVLVAASAMTSSTARQQAPPPSALVRSNERE
                     TIRQFTDLYCVACHNREDKTAGLALDAMSEEDVSRNSKAWERVVKKLVARQMPPDDEV
                     RPTQGAYNSIVAVLESTLDRAAALNPDPGRTLAFRRLNRTEYQNAIRDLLALSIDAST
                     MLPTDDSSHGFDNVTVGDLSPTLLDRYITAAQRISRLAIGSPGRSPGGDTIRIRPDLT
                     QEEHVLGLPIGTRGGALILYTFPQDGDYEIQVRLARDRNEHLEGLSEPHELELLLDRA
                     RRALFTVKPPKSETDHQTADGHLKARIHATAGPHNLGVTFLKNPSSLLETKRQPFQSH
                     YNYHRHPRISPAVYQVSITGPYDAKGHGDSPSRQRIFVCEPKESSEEETCARQILSTL
                     ARRAYRRPVNDEDLRKPMDLYREARARGDFDAGIEMALSAVLVNPQFLFRIEPDPPGI
                     APNRAYRIPDVQLASRLSFFLWSSIPDDELLDLAERGELSKPEVLERQALRMLADPRS
                     QNLVSNF"
     gene            1719..1841
                     /locus_tag="VHS97_13955"
     CDS             1719..1841
                     /locus_tag="VHS97_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449359.1"
                     /translation="MIELADRKGGYAVLLLILILLYRLEHHGQYVSSRTEERRL"
BASE COUNT          382 a          605 c          671 g          425 t
ORIGIN      
        1 gaagttgctc accaggtttt gcgaccgcgg gtcggccagc atgcgaaggg cctgccgctc
       61 gagcacctcg ggtttactca gttcgccgcg ctcggccaga tcaagcagtt cgtcgtccgg
      121 aatgctgctc cagaggaaga acgacaagcg agacgcaagc tggacgtcgg ggatgcggta
      181 ggctctattg ggcgcgatgc ccggcgggtc cggttcgatg cggaagagga attgcgggtt
      241 cacgagcacc gcgctcaagg ccatctcgat acccgcgtcg aagtctcccc gtgcgcgtgc
      301 ttcccggtac aagtccatcg gctttcgaag gtcttcgtcg ttcaccggcc gccggtacgc
      361 ccggcgcgca agggtggaga ggatttgcct ggcacacgtt tcctcttcgc tcgattcctt
      421 cggctcgcag acaaagattc gttggcggct gggcgagtcc ccatgcccct tggcgtcgta
      481 gggccccgtg atcgagacct ggtagaccgc aggtgagatg cgggggtgcc ggtgataatt
      541 gtagtgggat tggaacggct ggcgcttggt ctcgagcaac gacgaaggat ttttgaggaa
      601 cgtcacgccg aggttgtgcg ggccggccgt cgcgtggatt cgggccttca gatgcccgtc
      661 ggccgtctga tggtcggtct ccgatttcgg cggcttcacc gtaaacagtg ctcttcgcgc
      721 gcggtcgagc aatagctcca gctcatgcgg ctcgctcagg ccctcgagat gctcgttgcg
      781 gtcccgcgcc agccggacct ggatctcata gtcgccgtct tgaggaaacg tgtacaatat
      841 caaggccccg ccgcgtgtcc cgattggcag accaaggacg tgttcctcct gagtcaggtc
      901 cgggcggatg cggattgtgt cgcctccggg tgaccgacca ggacttccga tggccagccg
      961 gctgatccgt tgcgccgccg tgatataacg atccagcagc gttggcgaca gatcgcccac
     1021 cgtcacgttg tcgaatccgt ggctggaatc atcggtcggc agcatcgttg atgcgtcgat
     1081 ggagagcgcc aggagatcac ggatggcgtt ctggtactca gttcgattga gccgccggaa
     1141 cgccagtgtg cggcctgggt caggattcag agcggctgcg cgatcgagcg tgctctcgag
     1201 aacggcgacg atcgagttgt aggcgccttg ggtcggacgc acctcgtcgt ccggcggcat
     1261 ctggcgagcg acgagcttct tgacgactcg ctcccacgcc ttcgagttgc ggcttacgtc
     1321 ctcttcgctc atcgcgtcga gggccaaccc tgccgtcttg tcctcgcggt tatggcatgc
     1381 gacgcaatac aggtccgtga actgacgaat ggtttcgcgt tcgttcgaac gtacaagcgc
     1441 ggaaggaggt ggggcttgct ggcgagctgt actcgacgtc atggcgctcg ctgccacgag
     1501 cacgaccgat atcccgccaa gagtgcgagt tccccaccga ttcatcaggc ctgactccga
     1561 cattattgaa cgaaatcgag gaggataagg tcgcggcgca agcgactggc acagcccccg
     1621 ggacggcgcg gcgcccaggc aggaagatct ccagtttcga ggtcagaatc agtctatctt
     1681 accgcgttac ggcagccaag gatagacgtt gaaccctgtt gatcgaactg gccgaccgca
     1741 agggcggata tgcggtcctg ctcctaatct tgatcctgct ctaccgcctc gagcaccacg
     1801 gccagtatgt ctcaagccgc acggaggagc ggcgactctg atcagcccgc acaatgtccg
     1861 tcgcgttgcg attcctttcc gttgccacac tcggcaaaga aattacaatc agtgtccagt
     1921 ggggcagtga agcggttttg ttaatttgcg ccatgagtgg atgcgcactc ggctacggag
     1981 atcacagcaa cttggcattc gtggaaggat ttctgatgca tcagatcaag tgcgacattt
     2041 gcgagaaggc ggcggaaatc catgagacga gggtcgatgg agg
//
LOCUS       DATAQL010000801         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2829931, whole genome shotgun sequence.
ACCESSION   DATAQL010000801 DATAQL010000000
VERSION     DATAQL010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829931"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VHS97_13960"
     CDS             <1..322
                     /locus_tag="VHS97_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449360.1"
                     /translation="IVPGYENEPPAPSLRSIVRKSALLNVVIVLTSFPVLIFEGGPKA
                     VVPTLEIMAGITLVIWTATFALFSLVSLPRIFRTPASTVKRLDPHHPAEDAGVADRWL
                     DGPV"
     gene            complement(415..1035)
                     /locus_tag="VHS97_13965"
     CDS             complement(415..1035)
                     /locus_tag="VHS97_13965"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX3449361.1"
                     /translation="MTRRKVGILVFSEVEVLDFCGPFEVFSVTRLDEDRRRQDPSPYE
                     VLIIAESPGVVVATGALKVVPDHGLDVCPSLDVLVVPGGWGTRREMKNDRLIHWLSER
                     AREVTTLTSVCTGSLLLGKAGLLDGKRATTHWRVLEEMRGLFPAVNVIDDQHVVEEGN
                     VITSAGISAGIDMALRVVARHHGDAVARATARYMEYPFPEDNRRRV"
     gene            complement(1032..1640)
                     /locus_tag="VHS97_13970"
     CDS             complement(1032..1640)
                     /locus_tag="VHS97_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX3449362.1"
                     /translation="MAPEPLVPLDGPIKELAVGATKGLTTDEDKARAIYDKVTGMMKY
                     DKSGTGWGRGDAMYACDAKRGNCTDFHALLIGMARSVDIPARFAIGLPLPESRQSAEI
                     PGYHCWAELYVERRGWVPVDSSEASKNPAKRDYFFGHHDENRLEFSRGRHLTLTPAQQ
                     GPPLNFFVYPYAEVDGRPHDAVDLKFTFTDRGRPSPDLGGRR"
     gene            complement(2045..>3193)
                     /locus_tag="VHS97_13975"
     CDS             complement(2045..>3193)
                     /locus_tag="VHS97_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3449363.1"
                     /translation="LVTDMITQPRFAEQDFERLRNEALDYITKSLRGNDDEQVGKWTL
                     QVEIYKDHPYGHPDRGTAEGLRSITLGDVKEFHRRHYTRGALKLGLAGGFSETTPGLC
                     DRAFAGLPASRLEIPALPSPRMPKGLEVTIVEKPADATAISIGFPIDVTRRDDDFYAL
                     AVANSYLGEHRTFNGKLMQDLRGKRGLNYGDYSYIEDFIQQGQSTFPIPNNPRRQAYF
                     SIWIRPVPADKSVFALRAALWELDRLIERGISAEDFETTRKYLLNYSKLWVQTLSRRL
                     GYAIDGEFFGTKDLVSELAERLPKLTVDQVNAAVRKHLKSEGMTVAIVAKDAAGLREL
                     LVSGKATPLVYDTQGTPEDVLAEDKEIAVFPLKNVSVRIVPVGQMFEK"
BASE COUNT          624 a          965 c          936 g          668 t
ORIGIN      
        1 gatcgtcccc ggctacgaga atgagccgcc ggctccgagc ctgcggagca tcgttcgtaa
       61 gtcggcactt ctcaacgtgg tgatcgttct gacttccttc ccggtcttga tctttgaagg
      121 ggggccgaaa gcggtcgtcc cgaccctgga aatcatggcg ggaatcaccc tcgtgatctg
      181 gaccgccacg ttcgcgctgt tttcattggt ttcactcccc cggatcttcc ggacgccggc
      241 ctctactgtg aaacgacttg atccccatca tccagcggag gacgcggggg tcgctgatcg
      301 ttggctggac ggaccggtat gacggataca gacggcacgg gtttgttgag agatcaagaa
      361 tcgaaacgac ggactcgtct ctatgctcgt ggtccccgag cgactcgtga ggaatcacac
      421 ccgcctgcga ttgtcctccg gaaatgggta ctccatgtac ctggccgtcg cccgagcaac
      481 ggcatcgcca tgatgtctgg cgaccacgcg aagcgccatg tcgatgcccg ccgatatccc
      541 cgccgacgtg atcacattgc cctcctcgac gacgtgctga tcgtcgatca cattgactgc
      601 cgggaacaat cctcgcattt cctccaagac tcgccaatgc gtcgtggcac gtttgccgtc
      661 cagcaatcca gctttcccga ggagtagcga cccggtgcat acggaggtca gcgttgtgac
      721 ctcacgagcc ctctcgctga gccaatggat cagccgatcg ttcttcattt ctcggcgggt
      781 tccccagccg cctggaacca ccagcacgtc caacgacggg cagacatcta ggccgtgatc
      841 agggacgact ttcagagctc cggtagcaac cacaacgccc ggactctcag caatgatgag
      901 gacttcgtag ggtgacggat cttgccgcct ccggtcttca tccagcctcg tgacagaaaa
      961 gacctcgaac ggtccgcaga agtcgaggac ttcgacttcc gagaagacga ggattccaac
     1021 cttgcgacgg gtcaccggcg acctccgaga tcgggcgagg gacgcccccg gtccgtgaac
     1081 gtgaacttga ggtcgaccgc atcgtgcggt ctgccatcca cctcggcgta tgggtacacg
     1141 aagaaattta gaggtggtcc ttgctgagcg ggggtcaggg tcaggtggcg gccacggcta
     1201 aattccagcc gattctcgtc gtggtgtccg aaaaagtaat ctcgtttcgc agggttcttc
     1261 gacgcctcgc tgctgtccac tggcacccag ccccgccgtt ccacgtaaag ctcggcccag
     1321 cagtggtagc cggggatttc ggctgactga cgagattccg gtagcggcag accgatagcg
     1381 aagcgggccg ggatgtctac cgagcgagcc atgccgatca gaagggcatg gaagtcggtg
     1441 cagttgcctc gcttcgcatc gcaggcatac atggcatctc cccggcccca tcccgttccg
     1501 ctcttgtcgt acttcatcat gccggtcacc ttatcgtaga tcgcccgtgc cttatcttca
     1561 tcggtcgtta atcccttcgt tgccccgaca gcaagctctt tgatcgggcc atccaggggg
     1621 accagtggct ctggagccaa ttgatttctc cgatcaacgg cactcaacga ctcgcgacgg
     1681 ccgctgtttt ccctgcgggt cgcctcgatg gccaaagtga tttggaccga tcctttcggt
     1741 ccatcgacac gagcatgaag gcattggtta ccgaaacgtg attctcgacc gatggtgatc
     1801 gggaccgggg ccatgattga gatctgatga attgtttgat tggcgtcagt ttggggcagc
     1861 ggcacccagg cttcgagggt ctttgcatcc cttgggacgt ctcggatcgt cgcctgatag
     1921 gtcaacagaa aggttcggga tttcagaggt cgctcggacc cggagccaac ctgagaagcg
     1981 aaatacaccg agacaacgcc gaggatcagc ggcaaccgca gacaagctgt catcctttcc
     2041 accactattt ctcaaacatc tggccaaccg gcacgatccg gacagaaacg tttttgagcg
     2101 ggaagacggc gatctctttg tcctcggcca ggacgtcttc cggcgtgcct tgcgtgtcat
     2161 acacaagcgg cgtggccttg cccgagacga gtagctcgcg caggccggcg gcgtcctttg
     2221 ccacaatcgc gaccgtcatc ccctctgact tcaggtgttt gcgcaccgcc gcattcacct
     2281 ggtcgaccgt cagcttcggc agccgttcgg ccagctcgga gacaaggtcc ttggtcccaa
     2341 agaattcacc atcgatcgcg taaccgagcc gccgcgacaa agtctggacc cagagcttgc
     2401 tgtaattgag cagatacttg cgggtcgtct cgaaatcttc cgcggaaatt ccccgttcga
     2461 tgagccggtc aagctcccac aacgctgcgc ggagcgcaaa gaccgattta tcagcgggca
     2521 ctggcctgat ccagatcgaa aagtaggcct gcctgcgcgg attattgggg atgggaaatg
     2581 tgctttgccc ctgctggatg aaatcctcga tgtacgagta atcaccgtaa ttgaggccgc
     2641 gcttgccgcg gaggtcttgc atcagcttgc cgttgaaggt gcggtgctcg cccaagtaag
     2701 aattggcaac ggccagggcg tagaagtcgt cgtcgcggcg ggtcacatcg atcggaaagc
     2761 cgatcgagat agccgtcgcg tctgccggct tttcgacgat cgtgacctcc aggcccttgg
     2821 gcatgcgcgg ggacgggagc gccggaattt cgagtcggga cgcggggaga ccggcaaacg
     2881 cgcgatcgca aagcccgggc gtcgtctcac tgaagccacc ggcaagaccc agtttgaggg
     2941 ctcctcgagt gtagtgccgg cggtgaaatt ccttgacgtc acccaaggtg atcgacctca
     3001 agccttcggc agtcccgcga tcgggatgac catagggatg atccttgtag atctcgacct
     3061 ggagcgtcca tttgccaacc tgctcgtcgt cgttgccgcg cagtgacttg gtgatgtaat
     3121 cgagcgcttc gttgcgtagc ctttcaaaat cttgctccgc aaaccgcggc tgggtgatca
     3181 tgtcggtcac gag
//
LOCUS       DATAQL010000802         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_386470, whole genome shotgun sequence.
ACCESSION   DATAQL010000802 DATAQL010000000
VERSION     DATAQL010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386470"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VHS97_13980"
     CDS             complement(<1..878)
                     /locus_tag="VHS97_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX3449364.1"
                     /translation="MLSRVAENLFWMSRYLERAENVARLLDIGLYLELDAASLTADDG
                     SAPAEIALHILACREAFHSAHPTSDRNVVLGFLTFDRANPQSILSVIARARENARGTQ
                     ENLGVDAWSEVNRLYLYLSGQRAQRRFQASPSQFYTTIKQACILFDGLVQNTLPRDEA
                     YHFLELGRYLERVNVMGRILHAKCKSHNQATDSDAAMQLVRWTGLLRSCSAYGAYLRV
                     ERDKVEPVGVIRFLILNADFPRAVRFCVERCRESLQEIAGGDDDEYGSEAERLLGRLD
                     SELRYIDVGEIFERGL"
     gene            complement(880..2388)
                     /locus_tag="VHS97_13985"
     CDS             complement(880..2388)
                     /locus_tag="VHS97_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX3449365.1"
                     /translation="MTQPNGEAALFGRYCLAAYDEMFDSPATPRAHYASLHESLIELG
                     PEELERRDRVADLTMRQQGITFTVYGRDQGVERIIPFDPIPRLIAPNEWDRIERGLKQ
                     RVRALNLFIHDVYHERLILKDRIVPPELVFGATGYRRECVGLQVPRDIYVHVSGIDLI
                     RHSDGNFLVLEDNGRTPSGVSYVLKNRQVMKQVFPLLFSQYNVRPVDNYTDNLLAVLR
                     SIAPPGCDDPTVVVLTPGIYNSAYYEHSFLARQMGVDLVEGRDLVLDRGQIFRRTTRG
                     LQRVDVIYRRVDDDFLDPLTFRSDSMLGVAGLIGAYHSGNIGLANALGTGVADDKGIY
                     PFVPEMIRFYLREEPILANVETFRPLIPSHRQHILANLEKLVVKAVDASGGYGMLIGP
                     ASTKEQRDEFARKIEANPRGYIAQPTINLSRHPTLVDGRLDGRHVDLRPFVLYGEEII
                     VMPGGLTRVALPEGSLVVNSSQGGGTKDTWVLHDNAARLAPADAPPEDCART"
     gene            complement(2503..>3161)
                     /locus_tag="VHS97_13990"
     CDS             complement(2503..>3161)
                     /locus_tag="VHS97_13990"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX3449366.1"
                     /translation="VALFERILHNKAHLCRPGQPKRSRRSGAMMERAKKRCAWAKDEL
                     SIQYHDQEWGVPVHDDRLWFEFLVLEGAQAGLSWDTILRKRENYRRAFADFDPVVIAQ
                     YGDAKIEELLSDAGIVRNRLKVTSAIRNARMFLTLQEEFGSFDQYIWPFVGGRPVQNN
                     WRSLAEVPARTEISDALSKDLKRRGFTFVGSTICYALMQATGLVNDHLVDCFRSSAAK
                     "
BASE COUNT          646 a          960 c          951 g          604 t
ORIGIN      
        1 aagagcccgc gctcaaagat ctcacccaca tcgatataac gcagctcgct atcgagccgg
       61 ccgagcaacc gctcggcctc actgccgtat tcatcgtcgt cgccaccggc gatttcctgg
      121 agcgattccc gacagcgctc aacgcaaaag cggacggccc gcggaaagtc ggcgttcaag
      181 ataagaaacc ggatgacgcc gaccggctcg accttgtcgc gctcgacccg caagtaggct
      241 ccataagccg agcagcttcg caggagtccc gtccaccgga ccagttgcat cgccgcatca
      301 ctgtccgtcg cctggttgtg gctcttgcac ttggcgtgaa ggatccgtcc catcacgttg
      361 acgcgctcga ggtaacgccc cagttccagg aagtgatatg cctcgtcccg cggcagagtg
      421 ttctgcacca agccgtcgaa caggatgcag gcctgcttga tcgttgtgta aaactgggac
      481 ggactcgcct gaaaccgccg ctgggctcgc tggccggaga gatacaggta gagccggttc
      541 acttcactcc aagcgtcgac acccaggttt tcctgggtcc cgcgcgcatt ttcgcgggct
      601 cgggcgatca cgctcaggat cgattgagga ttggctcggt cgaaggtcag aaagccgagg
      661 accacgttgc gatcggaggt cggatgcgcc gagtgaaatg cctcgcggca ggccaggata
      721 tggagcgcga tttccgcggg cgccgagccg tcgtcggcag tcagtgacgc ggcatcgagc
      781 tcgaggtaga gcccgatgtc gagcaggcga gccacgttct cggcgcgctc gagatagcgg
      841 ctcatccaga agaggttttc cgcgacccgg ctgagcattt caggtcctcg cgcagtcctc
      901 gggcggagcg tcggccgggg cgagacgcgc cgcgttgtcg tgcaggaccc aggtgtcctt
      961 ggtgccacca ccctgcgagc tgttgaccac caggctcccc tcaggcagcg cgacacgcgt
     1021 caagcctcct ggcatgacga ttatttcttc gccgtaaagc acaaacggac gtaagtcgac
     1081 gtggcggccg tcgagccggc cgtccaccag tgtcgggtgc cggctcaggt tgatcgtggg
     1141 ctgcgcgatg tagccgcgag ggttggcctc gatcttgcgc gcaaactcat cacgttgttc
     1201 tttggtcgat gccgggccga tcagcatacc gtatccaccg gacgcatcga ccgccttgac
     1261 gaccagtttt tccaggttcg ccagaatgtg ctggcggtgc gagggaatca ggggccgaaa
     1321 cgtctctaca ttcgcgagga ttggctcttc gcgcagatag aaccgaatca tttcgggaac
     1381 gaaaggatag attcccttgt cgtcggcgac ccccgtcccc agtgcgttgg ccagcccgat
     1441 attacccgag tggtaagcac cgatgagtcc agcgacgccc agcatactat cggatcgaaa
     1501 cgtaagtgga tcgaggaagt cgtcgtcgac ccgccgatag atcacgtcga ctctctgcag
     1561 accgcgcgta gtgcgcctaa agatctggcc gcggtcgagc accaggtcgc gaccttccac
     1621 cagatcgaca cccatttgcc gggccaggaa actgtgctcg taataagccg agttgtagat
     1681 accgggggtc aacacgacaa cggtcgggtc gtcgcaaccg ggcggggcta tcgaccgcaa
     1741 gaccgccagg agattgtcgg tataattgtc gacgggccgc acgttgtatt gcgagaacaa
     1801 gagcggaaac acctgcttca tcacctggcg attcttgagc acatagctga cgcccgaggg
     1861 cgtccggcca ttatcctcga gcacgagaaa gttgccgtcg gaatggcgaa tcaagtcgat
     1921 gcccgagacg tgaacataaa tatcgcgcgg gacctgaagg ccgacacatt cccgccggta
     1981 tccggtggcg ccaaagacca gttccggggg gacgatccgg tctttcaaga tcagccgctc
     2041 gtgataaacg tcgtgaatga aaagattgag cgcgcggacc cgctgtttga gcccgcgttc
     2101 gatccggtcc cattcattgg gcgcgatcag ccgggggatc gggtcaaacg ggataatccg
     2161 ctcgacccct tgatcgcggc cataaaccgt gaacgtgatt ccttgctggc gcatcgtcaa
     2221 atcggccacc cgatcgcggc gctcgagctc ttcggggccc agctcgatca gcgactcgtg
     2281 caacgaggcg tagtgggcgc gcggtgtcgc gggggaatcg aacatttcgt cgtaggccgc
     2341 caggcaatag cggccgaaca aggctgcctc gccgttcggt tgagtcacat gcatcttggg
     2401 ttgggtttga atcggacaac aaacttttat atcaccggct tggcccagaa tccagcctga
     2461 aagcatcggc gcgagtgatt cgccatgaac ataaacagtg cgttacttgg ccgcagagct
     2521 tcgaaagcag tcgaccaagt ggtcgttcac gagcccggtt gcctgcatca gggcgtaaca
     2581 aatggtcgag ccgacgaacg tgaacccacg acgcttcaag tccttactca atgcatcgga
     2641 aatctcggtg cgggcgggaa cttcggcaag ggaccgccag ttgttttgaa ccggccggcc
     2701 accgacaaac ggccagatgt actgatcgaa gctgccgaac tcctcctgga gcgtgaggaa
     2761 catgcgggca ttccggatgg ccgatgtgac cttcaggcga ttacggacaa ttccggcgtc
     2821 gctgaggagc tcctcgatct ttgcgtcccc gtactgggcg atcacgacgg gatcgaagtc
     2881 ggcgaaagcc cgccggtaat tctcacgctt tcgcaggatc gtgtcccaac taagcccggc
     2941 ctgggcgcct tccaggacca aaaactcaaa ccaaagccgg tcgtcgtgga ccggcactcc
     3001 ccactcttgg tcgtggtact ggattgacag ctcatccttg gcccacgcgc atcgtttctt
     3061 cgcccgctcc atcatcgctc cgctacgtcg agaacgcttt ggttggcccg gtcgacacag
     3121 atgagcttta ttgtgtaaga tccgttcgaa taacgccact c
//
LOCUS       DATAQL010000803         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1456527, whole genome shotgun sequence.
ACCESSION   DATAQL010000803 DATAQL010000000
VERSION     DATAQL010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456527"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1000)
                     /locus_tag="VHS97_13995"
     CDS             complement(323..1000)
                     /locus_tag="VHS97_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449367.1"
                     /translation="MKIPVTCARCGKRYEVDATFAGKRGKCATCGERMTIPDKDQATS
                     APPAEPDSYQLDDARDSEQSTSFAQAQGSDGLEEPEPRRRVKKKNPRSSGRNGSDRAG
                     SHPALSTRATLIGLACAVVVLVSIAIFVPGARLNVGRAIALAGLILFFYGYGSGAYIA
                     FTEDDLYGWLYLFFPPYAAYYYISRWEEMSSRLVMLVLGLALLAGGGRLLEMERPPPA
                     AENGATA"
     gene            complement(1125..2564)
                     /locus_tag="VHS97_14000"
     CDS             complement(1125..2564)
                     /locus_tag="VHS97_14000"
                     /inference="COORDINATES: protein motif:HMM:NF020855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449368.1"
                     /translation="MSPILKRTLVGLVLAIVAASAARAGDLAGGVKLKTFVRPKKSSS
                     TPDRAFEYVQKVADFSLFDGQTRITPSGVGYVCLLERAQGDQVLVTIRSQGLRGWVPA
                     GSLVPLNHADAYFSQQTQATPRDAFAFLMRGVVRYENDDLDRAFADVNEALRLDPKYV
                     AALIERAYLWQCRDRLDLALADVNQGIQLDSRNSYAYVERGVFHFGMKDYGKALRDFE
                     QAARLGSRSAVIHLCKGMIHVERGEGEPAIAEFNLAIQLDTKRLDAYIGLATVYLLRS
                     DSHKALEVFNRAIEVDPRSAEAHEARAVYFLSHGKHDKALEDLNEAVRLDPTTASHLR
                     VRARVFFEKGEFARALADLDGATRLEPSDAEGHQGRAWILATCPDAKIRNGSQAVVSA
                     TRACELTDWKVPHALTTLAAAFSETGNFAAAVEWQQKALDLAADNSAQKPEYRRLLGR
                     YMAKKPYHRLGVLEEIGIQKTSTASKRGG"
     gene            complement(2605..>3155)
                     /locus_tag="VHS97_14005"
     CDS             complement(2605..>3155)
                     /locus_tag="VHS97_14005"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449369.1"
                     /translation="VYAAIASMFLMRGNTNKALKVLDQAVEIDPLCADSHGNRAVILL
                     SLGQYGKALDDLDEVVKVAPNSVRALRERAWILATCTDAKVRNGEQAVNSATRACELT
                     DWKDPHGLSTLAAAHSESGHFEDAVKWQQKAIELLIDTNPEKHEYRKSLDRYKANKPY
                     YRLSLLEEIGVPIPRPTAKKVD"
BASE COUNT          624 a          946 c          937 g          648 t
ORIGIN      
        1 gagccgatcc acgagttgat ttgagcggcc gtgccccgat cgcgaagcgc gtgcgtggag
       61 ctgttgaaaa gttgacggcc cgcgcttagg caatcatatg acgcgcgtaa gtgcaatgcc
      121 ttcaaattag cagtaactta cgggtgcata gcaatttatg atcccctcct ccctcgtggc
      181 tcaaaaagac gggttttcgt caccactagc tccctctcct tggggagagg gagccaataa
      241 catgcatcgg ccgcaagtgt cgcgtcgcga cgttgggatt ggcgacgcag cccccaagct
      301 cattcgccgc catcacgcgg gctcaggccg ttgctccgtt ctcggcggcc ggtggagggc
      361 gttccatctc gagtagccgc ccgccgccgg ccagcagagc cagaccgagg acgagcatga
      421 ccaaacggct gctcatctct tcccatcgac ttatatagta ataggctgca taaggaggga
      481 agaacaggta aagccagccg taaaggtcgt cttcggtaaa tgcgatgtaa gcgccggatc
      541 cgtaaccgta gaaaaagagg atcaagcccg cgagcgcgat ggcccgaccg acgttcagcc
      601 tggcgccggg tacgaagatc gcgatgctga cgagcaccac gacagcgcag gccaatccga
      661 tcaaggtcgc acgcgttgac aaggcaggat gcgaccccgc tctatcactc ccgttcctac
      721 cggaactcct ggggtttttc tttttcactc gccgacgtgg ctcgggttct tcgaggccgt
      781 cgcttccctg cgcctgcgca aacgaggttg actgctcgga atcacgggca tcatcgagct
      841 gataggaatc tggttcagcc ggaggtgcag aagttgcctg atccttgtcc ggaattgtca
      901 tgcgttcgcc gcaggtcgcg cactttcccc tcttgccggc gaaagtcgca tcgacctcgt
      961 accgtttacc gcagcgcgcg caagtgaccg gaatcttcat gtgccctcgt ctcacgacag
     1021 aataacagaa cgtgccaaag acggcctcat catgcattcg tcgtgaaacg cagtttatga
     1081 gcttggccgc ttacatggaa cgggcgacta acttgggcgg aagcttatcc gcctctcttc
     1141 gacgccgtgc tggtcttttg aattccgatc tcttccagca cgccgagacg atgatacggc
     1201 ttcttggcca tgtaccgacc gaggagccgg cgatactcgg gtttttgcgc actgttgtcg
     1261 gcagccaggt cgagtgcctt ttgctgccac tcgacggcgg cggcaaaatt gccggtctcg
     1321 gaaaatgcgg cggccagcgt cgtcagggcg tgcggcactt tccaatcggt cagctcgcat
     1381 gctcgagtag ccgagacaac cgcttgggaa ccattacgaa tcttggcgtc gggacaagtc
     1441 gccaagatcc aggctcgacc ttgatgcccc tcggcgtcgc ttggttcgag ccgtgtcgcg
     1501 ccgtccaggt ctgcgagagc tcgggcgaat tcacccttct caaaaaagac gcgggcgcgc
     1561 acccgcagat gacttgcggt cgtggggtcg agtcgaacgg cttcgttcaa atcctccagg
     1621 gccttgtcat gtttgccgtg agacaggaaa tagacagcgc gcgcctcatg ggcttcggca
     1681 cttcggggat caacttcgat cgccctgttg aaaacctcga gcgccttgtg cgagtccgat
     1741 cggagcaagt acaccgtggc cagaccgata tacgcgtcga gtcgcttggt gtcgagctgg
     1801 attgccaggt tgaactccgc gatcgccggt tccccctcgc cccgctccac gtggatcatg
     1861 cccttgcaaa ggtgaatcac ggctgagcga gagccgaggc gcgcggcttg ttcgaaatcg
     1921 cgcagggctt ttccataatc cttcatgccg aaatgaaaga cgccccgttc cacgtaagca
     1981 tacgagttgc gcgagtcgag ctggatccct tggttcacgt cggcaagagc caggtcgagt
     2041 cgatcgcggc attgccagag gtaggcgcgc tcgatcaagg ccgcgacata cttggggtcg
     2101 agacggagcg cctcattcac gtcggcaaag gcacgatcga gatcgtcgtt ctcgtatcgg
     2161 accactcccc gcatcaagaa cgcgaacgcg tcgcgcggtg tggcttgggt ctgctgggag
     2221 aaatacgcat cggcgtggtt gagaggaacc agggaaccgg ctggcaccca gccccgcagg
     2281 ccctggctgc gaatcgttac gagcacctga tcaccttggg ctcgttccag caggcagaca
     2341 taccccactc cgctcggtgt aattctcgtt tgaccgtcga agagcgaaaa atccgccact
     2401 ttctgcacgt actcgaacgc acgatccgga gtggagctcg atttcttggg acggacgaag
     2461 gtcttcaact tcacaccgcc ggcgaggtcg ccggcgcgcg cggccgaagc ggccacgatc
     2521 gccagaacga gcccgaccag cgtccgtttc aagattggcg acatgagcgg attccttttg
     2581 cgggcaaccg cgaggcgctg gagcttaatc aacctttttc gccgtcggcc gggggatcgg
     2641 aactccgatt tcttccagca ggctgagacg gtagtagggt ttgtttgcct tatagcgatc
     2701 caaagatttg cggtactcgt gcttttcggg gttggtgtcg atcagaagct cgatggcttt
     2761 ctgctgccac ttgacggcgt cctcgaaatg gcccgactcc gaatgcgccg ccgccagggt
     2821 tgaaagacca tgcggatctt tccagtcggt cagttcgcac gcgcgggtcg ccgaattcac
     2881 ggcctgctcg ccgttgcgga ccttcgcgtc ggtacacgtg gcgaggatcc aggcccgttc
     2941 ccggagtgcg cgaaccgaat tgggcgcgac tttcaccacc tcatcgaggt cgtcaagtgc
     3001 cttgccgtac tggcccagag acaataagat caccgcccga ttgccgtgtg agtcggcgca
     3061 caggggatct atttcgacag cttggtcaag aaccttcaga gccttgttgg tgttcccccg
     3121 catcaaaaac atcgacgcaa tcgcggcgta gacgt
//
LOCUS       DATAQL010000804         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2219799, whole genome shotgun sequence.
ACCESSION   DATAQL010000804 DATAQL010000000
VERSION     DATAQL010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219799"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..410
                     /locus_tag="VHS97_14010"
     CDS             90..410
                     /locus_tag="VHS97_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449370.1"
                     /translation="MGTSNGHWEGDTLVVEVADLNGQSWFDRAGNFASDSVRIVERYA
                     FADRDHLNYEATIDDKTVFTRAWKISLPLYRRKETNAQLTEFKCVEFAEEMLYGHLRK
                     GADK"
     gene            422..1468
                     /locus_tag="VHS97_14015"
     CDS             422..1468
                     /locus_tag="VHS97_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449371.1"
                     /translation="MSSRSITSALLLAAVLTSGVDARGQTLSSAPVARTASAAEAWTP
                     PKTPWGDPDLQGIYTSDDYIDTPLERPARFGDRLYFTEEELAEAAAKLAKQTEGILRG
                     TIRQGPLGGDREFLVNGERPRRPARQTSLIVDPPNGRMPPLTPEGRKRQSHMKLSTRN
                     WQTPAASWLDFDFYIRCISRGLAGSMLPSSYDNGTQIVQAPGFVTILNEKIHEARVIP
                     LDQRPDVGASIRGYLGDSRGHWEGNTLVVESTNFLDDTTSIGRNGSNAVATSDALRLV
                     ERFTRVDPDTIQYELTVDDPKIFTAPWTIAFQTRQERGYELFEYTCHESNYSMANMLS
                     GARAQEKAEATRQK"
     gene            1540..2592
                     /locus_tag="VHS97_14020"
     CDS             1540..2592
                     /locus_tag="VHS97_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449372.1"
                     /translation="MSSRSITSALLLAAVLTSGVDAQTLSSAPVARTASAADTWTPPK
                     TPWGDPDLQGIYTSDDYMNTPLERPMAYGERLYFTEGELAEAAGKLAKQAEAELQGTL
                     QREQGLRVYTVGDPRFDWGERPRRPARQTSLIVDPPNGKMPSLTPEGRKRQLQIRQTA
                     ISAQAPPASWKDYDFYIRCISRGLAGSILPSSYGNGTQIVQAPGFVMILHEKVHEARI
                     IPLDQRPHVGTSIRSYMGDSRGHWDGNTLVVETTNFLDGTTSIGRNGGNAVATSDALR
                     FVERFTRVDPDTMNYELTVDDPRIFTAPWKIAFPITQEPGYELFEYSCHETNYSMTNM
                     LSGARAQEKAEQAKQK"
     gene            2607..2996
                     /locus_tag="VHS97_14025"
     CDS             2607..2996
                     /locus_tag="VHS97_14025"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX3449373.1"
                     /translation="MICAQQTLRSAVRRNLSIVVAAVVALGVSACPAWAHHSFSAEFD
                     INSPVTLRGILTKMEWVNLHAWLHIDVKDEDGKTLAWMIETAAPNALLRRGYTKNSLP
                     IGAEVLVEGFRAKDGSNRANGSDHYVR"
     gene            3048..3812
                     /locus_tag="VHS97_14030"
     CDS             3048..3812
                     /locus_tag="VHS97_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449374.1"
                     /translation="MRHTLLSAFAVVVCFSGTFAQGDQAGTPTVAFDPHDLSGIWQGR
                     NRVLALSNQPPPRTAWGEAKFNSYKPSYGPRAIPPALGNDPQGNCDPLGIPRLLLFEN
                     NPWDFEIIQTRDRMLQLWDRHHVHREIWTDGRQPPNDMAPRWMGYAVGTWDGDTFVVN
                     STGFDERSWLDHFGNPHSDEMRLEERYRRVNHDTIELVITLTDPKAYTTPWVSGKKTL
                     TRENLTEFPDELFCVPSEEQEFNRRIRDPAGGVIPK"
     gene            complement(3859..4062)
                     /locus_tag="VHS97_14035"
     CDS             complement(3859..4062)
                     /locus_tag="VHS97_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449375.1"
                     /translation="MWGHTGPTLAVPFGPLFDQFVGGREERFRRLDAERPRRQAGGIP
                     HLQQRSLGLCNIAGIDQHGYLPA"
BASE COUNT          955 a         1223 c         1260 g          760 t
ORIGIN      
        1 agacagatct cgcgattgtc tacgaatacc gaacggccaa ccggctgatc aatgttgcga
       61 ggcaccggga gccccacgtc gacagctgga tgggcacatc gaacgggcat tgggaaggtg
      121 acacgctggt tgtcgaggtg gccgacctga acggccaatc gtggttcgat cgggccggga
      181 attttgccag cgacagcgtg cgcatcgtcg agcgttacgc gttcgccgac cgggatcacc
      241 tcaactacga agcgaccatc gacgacaaga cggtctttac gcgcgcgtgg aaaatcagtc
      301 tgccgctcta tcggcgtaag gaaacgaatg cgcagctcac ggaattcaag tgcgtcgaat
      361 ttgccgaaga aatgctatat gggcatctgc gcaagggcgc ggacaagtag aggaggttgg
      421 tatgagctcg cgatccataa cctcggcgct gctcctcgca gccgtgctca catcgggtgt
      481 cgacgcccgc ggccaaacct tgtcctcggc acccgttgcc cgaaccgcgt ctgcggccga
      541 ggcgtggacg ccgccgaaaa cgccgtgggg tgacccggac ctgcagggga tctacacgag
      601 tgatgactac atcgacacgc ctctcgagcg gcctgcccga tttggtgacc ggctctattt
      661 caccgaagag gagctcgccg aagctgcggc taaattagca aagcaaaccg aggggattct
      721 tcgggggacc atccgacaag gtccgcttgg tggcgacaga gagtttctag ttaacggaga
      781 gcggccgaga cggcccgcgc ggcaaacctc gctcatcgtc gatccgccca acggacgaat
      841 gccgccgctg acgccggaag gacgaaaacg acagtcgcac atgaagctat cgacgcgcaa
      901 ctggcagacg cccgcggcat cgtggctgga ctttgatttc tacatccgct gcatctcgcg
      961 cggcctcgcg ggctccatgc tcccctcgag ctacgacaat ggcacgcaga tcgtgcaggc
     1021 gccgggattc gtgaccattc tcaatgagaa gatccacgaa gcgcgagtca tcccgctgga
     1081 ccaacggccg gatgtcggag cgagcattcg cggctatctc ggagactcgc gcggccattg
     1141 ggagggcaac acgttggtcg tcgagtcgac gaatttcctg gatgacacga cgagcatcgg
     1201 cagaaatggc agcaatgcag tagcgacaag cgatgccctc cgtctcgtcg aacgcttcac
     1261 ccgtgtcgac cccgacacga ttcagtatga actgaccgtc gacgatccga agatcttcac
     1321 cgcgccctgg acgatcgcct ttcagaccag gcaagaacgg ggttacgagc ttttcgagta
     1381 cacgtgccat gaatcgaatt actcgatggc gaatatgctc agcggggctc gcgctcaaga
     1441 aaaggcggaa gcaacgcgac aaaagtagtt tacgcgcccg tggaaaatca gtctgccgct
     1501 ttatcggcgc aaggggcgcg gacaagtaga ggaggtggca tgagctcgcg atctataacc
     1561 tcggcgctgc tcctcgcagc cgtgctcaca tcgggtgttg acgcccaaac cttgtcctcg
     1621 gcacccgttg cccgaaccgc gtctgcggcc gacacatgga cgccgccgaa gacgccatgg
     1681 ggtgacccgg atctgcaagg gatatacacg agcgatgact acatgaacac gccgcttgag
     1741 cggcccatgg cctatggaga gcggctctac tttacggaag gggaactcgc cgaagcagcg
     1801 ggtaaattag caaagcaagc cgaggcagag cttcagggaa cccttcagcg tgagcagggc
     1861 cttcgcgttt ataccgtggg ggacccaaga tttgattggg gagagcggcc gagacggccg
     1921 gcgcggcaaa catcgcttat cgtggatccg ccgaatggaa aaatgccttc gttgacgccg
     1981 gaaggtcgaa agaggcagtt gcagatcaga caaacggcta tcagtgctca ggcacctcct
     2041 gcatcgtgga aagactatga cttctatatc cgctgcatct cgcgcggcct ggcaggttcg
     2101 atactcccct cgagctacgg caacgggacg cagattgtgc aggcgcccgg tttcgtgatg
     2161 attctccacg agaaggtcca cgaggcacgc attatcccac tggaccaacg gccgcatgtc
     2221 ggaacgagca ttcgcagcta catgggcgac tcccggggcc attgggacgg caacactctg
     2281 gtcgtcgaga cgacgaattt cttagacggc acgacgagta tcgggagaaa cggtggcaat
     2341 gccgtagcga cgagcgacgc ccttcgtttc gttgaacgct tcacgcgggt cgaccctgac
     2401 acgatgaact acgaactgac cgttgacgat ccgaggatct tcaccgcacc ctggaagatt
     2461 gcctttccca tcacgcagga acccggttac gagcttttcg agtactcgtg ccatgagacg
     2521 aattactcga tgacgaatat gctcagcggg gcgcgcgctc aagaaaaggc cgaacaagcg
     2581 aaacaaaagt agtttcaggc acgaacatga tttgcgccca gcaaacccta aggagcgccg
     2641 tgcgaagaaa tctttcaatc gtagtcgctg ccgtcgtcgc tctcggagtt agcgcgtgtc
     2701 ccgcgtgggc gcatcattca ttctcggcgg agttcgacat caacagccca gtcacgcttc
     2761 gcggaattct aacaaagatg gaatgggtta atctccacgc atggctccac atcgacgtga
     2821 aggatgagga cggcaagacc ctggcatgga tgatcgagac agctgccccc aatgccctcc
     2881 ttcggcgggg ctatacgaag aattcactgc caatcggcgc agaggtcctc gtcgaaggtt
     2941 tccgagccaa ggacgggagt aatagggcaa atggcagcga ccattacgtt cgctgacggc
     3001 caaatgcttt ttgtcggagg ccagccggac gcaaggtgaa caggcgaatg cgacacacac
     3061 ttctttcagc ctttgcagtg gtggtatgct tttcgggcac ttttgcccag ggcgaccaag
     3121 cgggaacgcc caccgttgct tttgatccac atgatctgtc tggaatctgg cagggccgga
     3181 accgggttct cgcgttgagt aaccaacctc ccccaagaac cgcatggggc gaggcgaagt
     3241 tcaattcgta caagccatca tacggtccgc gtgcgattcc gcctgcgctg ggcaacgatc
     3301 cgcagggcaa ctgcgatccg ctcgggattc cacgattgct tttgttcgaa aataacccgt
     3361 gggacttcga gattattcag acccgtgacc ggatgctgca gctttgggat cggcatcacg
     3421 tgcatcgcga aatctggacc gatggtcggc agcctccaaa cgacatggcg ccgcgctgga
     3481 tgggttacgc tgtcggaacc tgggacggag acacattcgt cgtaaattcg accggtttcg
     3541 acgaaagatc gtggctcgat catttcggca accctcacag cgacgagatg cgtctggagg
     3601 agcgttaccg gcgggtgaac cacgacacga tcgaactcgt catcactctg accgatccca
     3661 aggcttatac gacgccctgg gtcagcggca aaaaaacttt gacgcgtgag aacctcacgg
     3721 agtttcccga cgaactgttc tgtgtgccat ccgaagagca ggagttcaac cgtcgaatca
     3781 gggacccagc tggcggcgtt atccctaaat agggaacagc cgctcagctc aggacgttcc
     3841 gtgtggcgaa gaaatggatt acgcaggaag gtaaccgtgc tggtcaatcc cggcaatatt
     3901 gcacagaccg aggctacgtt gctggaggtg cgggatgccg cccgcctgac ggcgagggcg
     3961 ctcggcatcg aggcgccgga aacgctcctc gcgaccgccg acgaactgat caaaaagcgg
     4021 tccaaagggc accgccaggg taggaccagt atggccccac aatcaaacag cctcgattgc
     4081 tgcgatgcac acgccacttc aggttcgggt cataagcggg agcgccgacc acgtcgacgg
     4141 cctgcctcgc acatggctgg tgtaccccaa taaccggacc ggggccggac cggccggg
//
LOCUS       DATAQL010000805         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_693716, whole genome shotgun sequence.
ACCESSION   DATAQL010000805 DATAQL010000000
VERSION     DATAQL010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693716"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..2976)
                     /locus_tag="VHS97_14040"
     CDS             complement(34..2976)
                     /locus_tag="VHS97_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3449376.1"
                     /translation="MPTTTSISSFTLNRLADRLSQESRAELYFDRGMRGIYATDASLY
                     QIEPLGVVVPRTTADVAAVVAIAAQEHVPILPRGAATSLSGQTVGPAIVIDFSKYLNR
                     IGIVDRDRMTVKVEPGVVLDRLNAHLKPLGLMFGPDVSTGDRATIGGMIGNNSAGARS
                     LRYGKTIDHVHSVEAILADGTSATLGPVPDEELDLICCRSDTVGRIHRAVRDTVAGHC
                     QAIRDRFPHILRRVSGYNLDEFIPGLPARPAGWKEEPWQFNLAKLIVGSEGTLTVVAA
                     AELKLVPLPAAQGLVVLSFATIPAALERLAEIVESGPVAVEMLDSMILDLAAQNPSFA
                     EHLNFSAGRPAAVLAAQFYADSRDALSEQAETLAAKFEGRPGVLGVRKRLVDADKDDF
                     WKVRKAGFSLLMGIVGDAKPIAFVEDTAVDPALLPKFYDRFRQIVERHGVKAACYGHA
                     DVGCLHIRPLINVKTLGGVATLRSIAREVSDLVVEFGGAMSGEHGDGLARSLWNRKLF
                     GPEVYAALEAVKSAFDPENRLNPGKVVADPDPGESLRIGPDYHPREPHPTLLDFSNQG
                     GFAGAVEMCSGVGACRKTSSGTMCPSYMVTRDELHSTRGRANLLRLAMTGELSGLGDG
                     FDDETLHEALDLCLQCKACKTECPSKVDMAKLKAEVLYQHYQDRARPLSHLILGQIFR
                     LNPIAASTAHFTNRALRNPLFKWLLETLAGIDRRRTLPTFAYEHFRKWFRMHQPDIRA
                     GGRGRLILLDDCFTTYNNPEVGIAAVRVLEAAGYEVELAGLECCGRPAISKGLLPLAR
                     RLAAANVQKLAAFARLNIPIVGCEPSCLVTFLDEYRDFRLGPDAELVASASVMVDAFV
                     ANSSFVPHLPLSARPGRVLVHGHCQQKAVIGTAGTLAALRRVPELEAKELDSGCCGMA
                     GSFGYESGHYEVSEALANRVLIPAAQADPTAQLVAPGFSCRSQIHGLAGIAAFHPIQI
                     LAEQLHSV"
     gene            complement(3038..>3491)
                     /locus_tag="VHS97_14045"
     CDS             complement(3038..>3491)
                     /locus_tag="VHS97_14045"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3449377.1"
                     /translation="SEAEIAGIMGGHMGMFARTQRLGRPLIEFIFRIDQAKDLQRRPD
                     VAFVSHARWPVRRRVPDVAVWDMVPDVAIEIVNPSNTANQVQEKIHDYLTAGVSLVWV
                     VYPRQQEVYVYKSPAQIKVLQLGQELDGGELIPGFRLPLAALFEDDPE"
BASE COUNT          709 a         1058 c         1110 g          614 t
ORIGIN      
        1 cggcgcgact gactcgtggg aagcggatca gcatcagacg gagtggagtt gctctgccag
       61 gatctgaatt gggtggaaag ccgcgatacc agccaggccg tggatctggc tgcggcagga
      121 aaagcctgga gcgaccagtt gggccgtcgg atctgcctgc gccgcgggga tcaagacgcg
      181 gttggcgagc gcttcactca cttcgtaatg gcccgattcg tagccaaaag accccgccat
      241 gccgcaacag cccgaatcga gctctttggc ctcgagctct ggcacccggc gcagggcggc
      301 gagcgtgccc gccgtgccaa tgaccgcctt ttgctggcag tggccatgca ccagcacgcg
      361 gccgggtcga gcggagagcg gcaaatgggg cacaaatgag gaattggcaa caaacgcgtc
      421 aaccatgacc gaggccgacg ccacgagctc ggcatcgggg cccaggcgga aatcgcgata
      481 ttcgtccaaa aacgtcacca ggcagctcgg ctcacagccc acgatcggaa tattcaggcg
      541 cgcgaatgcg gcgagctttt gaacgttagc ggcggcaagc cgtcgagcca gaggaagcag
      601 ccccttggaa atcgctggcc ggccgcagca ctcgaggccc gccagctcaa cttcatagcc
      661 tgcggcttcc agcacccgca ccgccgcgat gcctacctct gggttgttat aagtcgtaaa
      721 gcagtcgtcg agtaaaatca gtcgacctcg gccgccggcg cgtatgtcgg gctgatgcat
      781 gcggaaccat ttgcggaagt gttcgtacgc gaacgtggga agtgttctgc ggcgatcgat
      841 tccggcaagt gtttccagca gccacttgaa taacgggttg cgcaacgccc ggttggtaaa
      901 gtgagcggtc gatgccgcga tcggattcaa gcggaaaatc tggcccaaga tcagatggct
      961 caaagggcga gcgcggtctt ggtagtgctg gtacagcacc tcggccttga gtttggccat
     1021 gtcgaccttc gagggacatt cggtcttgca cgccttgcat tggaggcaca aatcgagcgc
     1081 ctcgtggagg gtctcgtcat cgaatccgtc ccccagcccg gacagttcgc cggtcattgc
     1141 caggcggagc aagttggcac ggccgcgcgt tgagtgcagc tcatcgcgcg tcaccatgta
     1201 gctggggcac atggtgccgc tcgaagtctt gcggcaggca ccgactcccg agcacatttc
     1261 gaccgcgccg gcaaatccac cctggtttga gaagtcaagc agcgtgggat gtggctcgcg
     1321 cggatggtaa tccggtccga tgcgcaggga ttcaccgggg tccgggtcag ccacgacctt
     1381 gccagggttc aagcggttct cggggtcgaa agccgacttg acggcttcaa gggcggcata
     1441 gacctccggc ccgaaaagtt tacggttcca aagactgcga gccaggccat cgccgtgctc
     1501 gccgctcatc gcaccgccaa actcgaccac caagtccgag acctcgcgcg cgatcgaccg
     1561 cagcgtcgcc acaccgccta acgtcttgac gttgatcaag ggtcggatgt ggaggcagcc
     1621 gacgtcggca tggccgtagc acgctgcctt aacgccgtgg cgttcgacga tctggcggaa
     1681 tcgatcgtag aatttgggca gcaaagcggg atcgacggcc gtgtcttcga cgaacgcgat
     1741 cggtttggca tcgccgacaa ttcccatcag gagagaaaag ccggccttgc ggaccttcca
     1801 gaagtcgtcc ttgtcggcgt caacgagtcg tttacgaacc cccagcaccc ccggccggcc
     1861 ctcgaatttg gccgccagcg tttcggcctg ctcactcagc gcgtcccgtg aatctgcgta
     1921 aaactgggcg gccaaaaccg cggctggccg cccggcgcta aagttgagat gctcggcgaa
     1981 cgacggattt tgagctgcca ggtcgaggat catgctgtca agcatctcga cggccactgg
     2041 accagactcg acgatctcag caaggcgttc gagggctgcg ggtatcgtcg caaatgaaag
     2101 cacaacgagc ccttgggccg ccggcaacgg gactagcttt aactcggccg cggcgacaac
     2161 cgtcagggtc ccttccgaac cgacgatcaa ctttgccagg ttgaactgcc agggctcttc
     2221 cttccaacca gcaggccgcg cgggcagccc cgggatgaat tcatcgaggt tgtagccgct
     2281 cacccggcgg agtatatgcg gaaaccggtc gcggatcgcc tgacaatgcc cggcaaccgt
     2341 gtcgcgcacc gcccgatgga tcctgccgac cgtatccgac cgacagcaga tcagatcgag
     2401 ctcttcgtcg ggaacgggac cgagagttgc gctggtgccg tcggccaaaa tggcctcaac
     2461 tgagtggacg tggtcgatcg tcttgccgta tcggagcgag cgggctccgg cggagttgtt
     2521 gccgatcatg ccgccaatcg ttgcccgatc accggtcgag acgtcaggcc caaacatcag
     2581 gcccagcggt tttaagtgtg cgttgagccg gtcgagcacc acacccggct cgacctttac
     2641 ggtcatgcga tcgcggtcga cgatcccaat tcgatttaga tatttagaga agtctataac
     2701 gatcgcagga cctacagtct ggcccgacag gctggtggcc gccccgcgcg gcaggatcgg
     2761 cacatgttcc tgggcggcga tcgcgacgac ggcggccacg tcggctgtcg tccgggggac
     2821 gaccacgccc aagggctcga tctgatagag gctggcgtcg gtcgcgtaga tcccgcgcat
     2881 tccgcggtcg aaataaagct cggcgcgcga ctcttggctc aagcggtcgg ccaggcgatt
     2941 cagggtgaag gaggagatcg acgtggtcgt gggcattggt cgcggactcg agcaagaggc
     3001 agaaggcacg gagctcgatc aaggaaaacc gacgcgatca ctcgggatcg tcctcgaaca
     3061 acgcggcgag aggaagtcgg aaaccgggga taagctcgcc accgtcaagc tcctggccta
     3121 gttgcagtac tttaatctga gcaggcgact tataaacata gacttcctgc tgacgagggt
     3181 agaccaccca gacgaggctg acaccggccg tcaagtaatc gtgaatcttt tcctgaacct
     3241 ggttggcggt gttggatggg ttgacgatct cgatggccac atcgggcacc atatcccaga
     3301 cggcgacgtc gggcacgcgc cggcgaacgg gccaacgtgc atgagagacg aaggcgacgt
     3361 ccggccgtcg ctggaggtcc ttcgcttgat caatgcggaa aataaactcg atcagagggc
     3421 ggcccagtcg ctgcgtccgg gcgaacatgc ccatgtgtcc gcccatgatc ccagcgatct
     3481 cggcctcgct c
//
LOCUS       DATAQL010000806         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3440354, whole genome shotgun sequence.
ACCESSION   DATAQL010000806 DATAQL010000000
VERSION     DATAQL010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440354"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VHS97_14050"
     CDS             <1..375
                     /locus_tag="VHS97_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449378.1"
                     /translation="ALDPATVIKQYVREHVASFPFGGVRIDWLKPVLPIYSRTLANAA
                     PLEWTEGHTVRVATAEGLILTKMVAFRPQDQMDIDTLLTANRDNIDVDLIREEWSPFA
                     STEAERTAWLEAAIDKRVVRRE"
     gene            478..1755
                     /locus_tag="VHS97_14055"
     CDS             478..1755
                     /locus_tag="VHS97_14055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3449379.1"
                     /translation="MNTHLRSKRRAFVLNASYLSIMQSIILASPLSAGEIPVVSAAEA
                     GLSEAKLAEVDRFMERQVADKKIAGGIVIVARDGKIGLFHAYGKMDLETNQPTRPDTI
                     FRIYSMTKAITTAAALNLVDAGKIGVDDPVSKYIPSFADLKVATPSGLRAPKRPMSIR
                     DLMLHTAGLTYGAPSDALKEAYDRLKPLQSKDLKEMTDKLAQIPLAFDPGTDWTYSAA
                     IDVLGRVIEVASGETLDVYLKKTIFEPLDMPDTAFNVPPEKLPRFAANYTRSTGGLKV
                     TDVPAKSAYAKEVTFFSGGGGLVSTAMDYLRFLTMIQNGGELGGHRILRPETVALMTT
                     NQLPKEAFPIHFDKEARLGTGFGFGFSVRTENTSWDPSGRLGEYGWGGAASTHYWVSP
                     ADKLIVITLEQIMPYQWDTEFGVKKIIYDAVNK"
     gene            1833..2426
                     /locus_tag="VHS97_14060"
     CDS             1833..2426
                     /locus_tag="VHS97_14060"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX3449380.1"
                     /translation="MTKQALKKDTRDAILEAGVKSVLEKGYHECGLAEILKEAGVPKG
                     SFYYYFASKEEFGLQIIEHWLENFAEAEASLGNELLSPLTRVRQFFEARCRLFKRLRR
                     KHGAVVGTLYLEMATHNETFRLRVEKLLVAWQERVTNCLVQAQEAGEIGADKDPAALA
                     EFALISWEGAVQRARTVQSIAPLDLFFMVIFGTVLVN"
BASE COUNT          526 a          742 c          702 g          488 t
ORIGIN      
        1 gcgctcgacc cggctaccgt catcaagcaa tatgtccgag agcacgttgc ctcgtttccg
       61 ttcggagggg ttcgtatcga ctggctcaag ccggtactcc ctatttattc ccggacactc
      121 gccaacgccg cacccctaga atggacggag gggcacacag tccgcgtggc caccgccgaa
      181 gggctgatcc taacgaagat ggtcgccttc cgcccgcagg accagatgga catcgatacg
      241 cttttgactg ccaaccgcga taatattgac gtcgacctca tccgcgagga atggtctcca
      301 ttcgcgtcga cggaagccga acgtacggcg tggttggagg cagcgatcga caagcgggtc
      361 gtccgacgcg agtgagtcgc cgctggtttc catccgactt ggcgattacg gtagtaagtc
      421 ctgatttgtg tcggcccatg tttgtcatgg cagcgggcta ttagcaaaag ggcgatcatg
      481 aatacacacc ttcgttcgaa gcggcgagcg ttcgtactca acgcgagtta cctatcgatt
      541 atgcagagca tcattctggc gtcgccgctc tcggcaggcg aaatcccagt agtatcagcg
      601 gccgaggctg gactctcgga agcaaagctg gccgaggtcg acaggttcat ggagcgccag
      661 gtcgccgaca agaagattgc gggcgggatc gtgatcgtcg ctcgcgacgg caagatcggc
      721 ttgttccacg cgtacggcaa gatggacctg gagaccaatc agccgacgcg gcccgacacg
      781 attttccgca tttattcgat gactaaggcc atcacgacgg cggctgctct caatctggtc
      841 gatgcgggca agatcggcgt ggacgacccg gtctccaaat acatccccag cttcgccgat
      901 ctcaaggtcg cgactccgtc gggcctgcgc gcgcccaagc ggccgatgag tatccgcgac
      961 ttgatgctgc acacagccgg cctcacctat ggagcaccct ccgacgccct caaggaagcc
     1021 tacgatcgat tgaaaccgct gcaatcaaaa gacctgaagg aaatgaccga taagctcgcg
     1081 cagataccgc tggccttcga ccccggcacc gactggactt actcggccgc gatcgacgtc
     1141 ttgggtcggg tgatcgaggt tgccagcggc gagacgctcg acgtttatct caagaagacg
     1201 atcttcgagc cgctcgacat gccggacacc gccttcaacg tcccgcccga aaagcttcct
     1261 cgtttcgccg ccaactacac ccgttcgacc ggcggcttga aagtcaccga tgttcccgcc
     1321 aaatcagcgt acgccaaaga ggtaaccttc ttctccggcg gcggcggtct ggtatcaacc
     1381 gccatggatt acctgcgttt cctcaccatg atccagaatg gcggtgaatt gggcggacat
     1441 cggattctcc gtccggagac agtcgcgctc atgacgacca accagcttcc caaggaagcc
     1501 ttccccatcc atttcgacaa ggaagcccgt ctgggcacgg ggtttgggtt tggcttctca
     1561 gtccgaactg agaacacgag ctgggatccg tccggccgct tgggcgaata tggctggggt
     1621 ggcgctgcct ccactcacta ctgggtcagc ccggccgaca aattgattgt gataacgctc
     1681 gaacaaatca tgccctatca atgggatacc gagttcggcg tgaagaagat catttacgac
     1741 gccgtcaata agtaagcaaa tcgccgctgc cgtccccatc cggcgggaca tactgcttga
     1801 tattctagac gaccggtcta ctatgagtgc gtatgacaaa acaagcgctc aagaaagata
     1861 cgcgtgacgc aattctcgag gcgggggtga aatctgtcct cgagaagggg taccacgagt
     1921 gcggcctggc ggagattctc aaagaggccg gcgtccccaa gggatcgttc tattactact
     1981 tcgccagcaa agaggagttc ggtctccaga tcatcgagca ttggctcgag aatttcgccg
     2041 aggccgaagc gagcctgggt aacgagttgc tctcaccgct cacgcgggtg cgccagttct
     2101 ttgaggcccg ctgccgactc ttcaagagac tgaggcgtaa gcacggcgcc gtggtcggga
     2161 cgctctacct ggaaatggcc actcacaacg agacttttcg cctgcgcgtg gaaaagctct
     2221 tggtcgcgtg gcaagaacga gtcaccaact gtttggtcca ggctcaagaa gccggcgaga
     2281 tcggcgccga caaagatcca gccgccctgg ccgagtttgc cctgattagc tgggaaggcg
     2341 ccgtccagcg cgcccgcacc gtccagagta tcgcgccgct cgaccttttc tttatggtca
     2401 tattcggaac cgtccttgtg aattaacacg accgccttgt ctcagacttg cccgcgat
//
LOCUS       DATAQL010000807         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_999259, whole genome shotgun sequence.
ACCESSION   DATAQL010000807 DATAQL010000000
VERSION     DATAQL010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999259"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VHS97_14065"
     CDS             complement(<1..1366)
                     /locus_tag="VHS97_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3449381.1"
                     /translation="MHTRIMAFLGIGLIGSLVSPGLGRAAEDKPPGTDRPANRLAKET
                     SPYLLLHAHNPVDWYPWGPEAFARAKSENKPVFLSVGYSSCYWCHVMERESFVDPEIA
                     KALNAGFVCIKVDREERPDVDQVYMAALQVFGPGGWPMSLFLTPDGRPFFGGTYFPPR
                     DREGSSGFLTIVNEVAKAWTKQQTEIDKTANQVTELVRKRLKVARNERKLPLAGTAAD
                     EGLKQLAEQFDSENGGFGFNPNNPRRPKFPQPVDLQFLLERHRAGLKTNESTDPLKMV
                     LFTLDRMARGGIRDQLGGGYHRYSTDRYWLVPHFEKMLYDNAQLASVHLLAFELTHDP
                     RWRDEAEATFGFIERRMTAPEGGFYSALDAETKGEEGAYYVWTRDQVKADLAGVPHAE
                     LFAQVYGLDGEPNFEGGRFVLREARTRAEQSKALSITPEELETRLRPLRFRLLAARDQ
                     RPAPL"
BASE COUNT          384 a          619 c          613 g          410 t
ORIGIN      
        1 acagcggtgc cggccgctgg tcgcgagccg ctagcagccg aaaccgcagc gggcgcagcc
       61 gcgtctccag ctcttccggc gtgatcgaaa gcgccttgga ttgctcggcc cgtgtccgag
      121 cttcgcggag cacaaaccgg cccccctcga aattcggctc gccgtccagt ccgtagacct
      181 gggcaaatag ctcggcgtgc ggtactcccg ccagatcggc cttcacttga tcgcgcgtcc
      241 agacgtaata cgccccctcc tctcccttgg tctcggcatc gagagccgaa tagaagcccc
      301 cttccggcgc agtcatccgc cgttcgatga acccaaaagt ggcctcggct tcgtcgcgcc
      361 agcgcgggtc gtgggtgagc tcgaacgcca gcagatggac cgacgcgagc tgggcgttat
      421 cgtagagcat cttctcgaag tgggggacca accagtaccg atcggtcgaa taccggtgat
      481 aaccgccccc cagttggtcg cggatgcccc cccgggccat ccggtccagc gtaaacagga
      541 ccatcttgag tgggtcggtc gattcgttcg tcttcagccc agcgcggtga cgctcgagaa
      601 gaaactgcaa gtcgacgggc tggggaaact tgggccggcg cggattattg gggttgaaac
      661 cgaacccgcc gttctcggag tcaaactgct ccgcgagctg tttcagcccc tcgtccgccg
      721 cagttcccgc gagcggcagc ttgcgctcgt tacgcgcaac cttgagccgc ttgcgaacga
      781 gctcggtcac ttggttggcc gtcttatcga tctccgtttg ctgtttggtc caggctttgg
      841 ccacctcatt tacaatcgtc aggaacccgg aagagccctc acgatcacgc ggtggaaaat
      901 aggttccgcc aaagaatggc cggccgtcgg gtgtgaggaa gagtgacatc ggccacccgc
      961 cgggtccaaa gacctggaga gcggccatgt aaacctggtc gacgtcgggc cgttcctcgc
     1021 ggtcgacctt gatgcaaacg aagcccgcgt tcaatgcctt ggcaatctcc gggtcgacaa
     1081 agctctcgcg ctccatgacg tggcaccagt agcaagagct gtagccgacc gatagaaaga
     1141 ccggcttgtt ctcgctcttg gctcgggcaa atgcctcggg accccacgga taccagtcga
     1201 ccgggttgtg cgcgtgtaac agcaagtaag ggctcgtctc cttggcgagc cggttggctg
     1261 ggcggtccgt tcccggcggt ttgtcctccg cggcgcggcc gaggccgggc gacaccagag
     1321 atccgatcaa tccaatccca agaaatgcca tgatcctggt gtgcatccga agttccttgg
     1381 ctgggcgcca ttgttgttga cggagactga agagttcgcg tcatcgctcg ctcgattcta
     1441 cgccccttcc gcccgcgtac caatcaagac gattgcgttt gctcgccgca caggtcgtca
     1501 tctctccgtt taatgcgaag caccagtagt ccccggtggc gttggcttcg tttcgcaaaa
     1561 tgatttcgtc gtcgcaagca tttgcgaaaa agcaacttgc ggctggcacc acgtggctgg
     1621 tttgggaaat actgccacgt ccaccgcagt cgccaacttc tcgttgcttt gtttgaatgg
     1681 cagtcagctg ggcgaccgtg acgtgaaccg agtcgaatcg actccgggtg atcgttgctt
     1741 attgatcagg aggagatagg gcggcatcgg ccaatcagtt gtaaaggtgg caagaatgtt
     1801 cgcatcttgt ggcctgcgca agatatccca atcatattcc cgtccgacgc atcagcactg
     1861 ttatgaccct gatcgcaacg atcggcctgg ggagcgctgt gatgcgatgt ctcgcgattg
     1921 ccgtggcctt cacggctacg gttattgggc aagaggcagg gagttcaacc actgaggaac
     1981 aaaccaaaaa gcgtgcggcg gccgttgcca gagggcgcgc ggaggc
//
LOCUS       DATAQL010000808         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_387164, whole genome shotgun sequence.
ACCESSION   DATAQL010000808 DATAQL010000000
VERSION     DATAQL010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387164"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VHS97_14070"
     CDS             <1..832
                     /locus_tag="VHS97_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449382.1"
                     /translation="QVLLDEPGSLPFKAHLGLGLDDLAGVARSQGQIDEARKLFQEAE
                     QLYSELVESDPELRESRFTLLHTRFNQAKMEEGELRFSRAAELWRRIRDQLRQLQRDG
                     LLEGRTDHSTNPSVVAAELAYCEAAPTAIKDLDFARSQPALVSARLLLLHARMFDQKT
                     NDQNRLAAVGEALTGLASTAPQELFELARYLAIFIKDLDSGRWPGFREAERQQLRARC
                     TSRGTELLRLAADRGFEDRSLIESAELAPLRNQPGVRSQAARLKPPTSEPADRVKTSE
                     "
     gene            complement(816..1946)
                     /locus_tag="VHS97_14075"
     CDS             complement(816..1946)
                     /locus_tag="VHS97_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3449383.1"
                     /translation="MASAQPIRVAIIGLGFGAEFIPIYQKYPGAEIHAICRRDKKGLD
                     EVGDRYGIKARYTEYRDLLKDSDVDAVHINSPIPDHAWMSVEALKAGKHVACTVPMGT
                     SIDECRQIVLAQRASGKIYMMMETVVYSREYLFVKELYDKGELGRLQFLRGSHQQDMD
                     GWPDYWPGLPPMWYATHCVSPCLAILGKHAQHVVCHGSGRIREELIGRYGSPFAIETA
                     TFAIKDSDVAAEVTRSLFDVARQYRESFDAYGSKKSFEWQQVENEDPVIHTKSTPEHP
                     VPEPEIPKKVKVPDYAHLLPEPIRRFTQPAAIQDAEHLSFLQGGGHGGSHPHLVHAFL
                     SAVNGDRPALPDAATSANWTMVGLCAHESALKGGEKIIIPTF"
     gene            2048..>2472
                     /locus_tag="VHS97_14080"
     CDS             2048..>2472
                     /locus_tag="VHS97_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3449384.1"
                     /translation="MATASEPKKVRVGFIGTGAVTAYHHLPGLLLDPRAELTAICDTV
                     PDVLEKRRAEWNVAYATTDPLALCASDKVDAVVIATPNDTHRPIAVAAAQAGKHVMCE
                     KPLGLSAHEVRAMYEAARDTGVVHMTAFTYRFAPAMRYLR"
BASE COUNT          476 a          745 c          762 g          489 t
ORIGIN      
        1 ccaggtcctc ctggacgaac cgggcagttt gcccttcaag gcgcacctcg ggctcggcct
       61 cgacgatctc gccggagtcg cccgatccca gggccagatc gacgaagcgc gaaagctatt
      121 ccaagaagcc gagcagctct attccgagct cgtcgagtca gatccagagc ttcgcgaatc
      181 tcggttcact ctcctgcaca cccggttcaa tcaggccaag atggaggaag gcgagctccg
      241 gtttagcagg gcggccgagc tctggcgccg cattcgcgat cagttgcgtc agctacagcg
      301 cgacggtctt ctggaagggc gaaccgacca ctccaccaat cccagcgtcg tggcggccga
      361 actcgcctat tgcgaggctg ctcccacggc aatcaaagat cttgatttcg cccgttccca
      421 gcccgccctc gtatcggccc ggttgctgct cttgcacgcg agaatgttcg atcagaaaac
      481 aaacgaccag aaccggctcg cggcagtcgg tgaggctctg accggcctgg catcgaccgc
      541 tccgcaagaa ctgtttgaac tggcgcggta cctggccatc tttattaagg atctcgattc
      601 ggggcgttgg cctggcttcc gtgaggcaga acggcagcag ctgcgcgcac gctgtacgag
      661 ccgcggtacc gaattgttgc gtctagccgc ggatcgagga ttcgaagacc gctcgctcat
      721 tgagagtgcc gaattggcac cgctgcgcaa tcagcccgga gtcaggtcgc aagccgcgag
      781 gttgaaacca ccgacgagcg aaccggccga ccgcgtcaaa acgtcggaat gatgatcttt
      841 tcgcccccct tgagcgccga ttcgtgggca cacagtccca ccatggtcca gttggctgag
      901 gttgccgcgt cgggcaaggc tggccggtcg ccgttgactg cgctcaaaaa ggcatgaacc
      961 agatgcggat gcgacccgcc atggcctccg ccttgcaaga aggaaaggtg ctcggcgtcc
     1021 tggatcgccg cgggttgggt gaaccggcga atcggttcgg ggagtagatg cgcataatca
     1081 ggcactttca ctttcttggg gatctctggt tcgggcacag gatgctcggg ggtgctcttg
     1141 gtgtggatca cgggatcttc gttttcgacc tgctgccact cgaagctctt cttgctgccg
     1201 tacgcgtcaa agctctctcg atattggcgc gcgacatcga acaggctacg ggtcacttcg
     1261 gcggcgacat cggaatcctt gatcgcgaaa gtggcggtct cgatggcgaa tgggctccca
     1321 taacggccga tgagctcctc gcgaattcgc cccgagccgt ggcagacgac gtgctgggca
     1381 tgtttgccca ggatcgccag gcacgggctg acgcagtggg tggcgtacca catcgggggc
     1441 aagccaggcc agtaatcggg ccagccgtcc atgtcttgct gatggctccc gcggagaaac
     1501 tgcagccggc ccagctctcc cttgtcgtaa agctctttga caaacaggta ttcgcggctg
     1561 tagacgacgg tttccatcat catgtagatc ttgccactgg cccgctgggc aaggacgatc
     1621 tggcggcatt cgtcgatcga tgtccccatt ggaaccgtgc aggcgacgtg cttgccggcc
     1681 ttcaaggcct cgaccgacat ccaggcatgg tcgggaatag gtgaattgat atgtacggca
     1741 tcgacatcgg agtctttcaa aagatcgcga tactcggtgt agcgggcttt gatcccatag
     1801 cggtcgccga cttcgtcgag tccctttttg tcgcgccggc agatcgcatg gatttcggca
     1861 cctgggtatt tctgataaat ggggataaat tcggcgccga aaccgaggcc gatgattgcc
     1921 acgcgaattg gctgtgcgct tgccatcgat cgatctccta tgtcccgaga gtgacttccc
     1981 ttgtgaagtt ccgtgcagga tagtaggaca accgactgaa ggcaagcacc cggaaaggag
     2041 agccagtatg gcgacagcgt ctgagcccaa gaaagtgcgt gtggggttta tcggcaccgg
     2101 agcggtgacg gcctatcatc atttaccggg tcttctgctg gatcctcgcg ccgagctgac
     2161 cgcgatttgt gataccgtgc cggacgtgct cgagaaacgc cgagccgaat ggaatgtcgc
     2221 ctacgcgacg accgatccgc tggcattgtg cgcgtccgat aaagtcgatg cggtggtgat
     2281 cgccacgcca aacgacaccc accggccgat cgcggtggcg gcggcccagg ccggcaagca
     2341 cgtcatgtgc gaaaagccgc tcggcttgag cgcccacgaa gtgcgcgcga tgtacgaggc
     2401 tgcccgcgac accggcgtcg tgcacatgac ggctttcacc taccgctttg cgcccgccat
     2461 gcggtacctc cg
//
LOCUS       DATAQL010000809         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_999292, whole genome shotgun sequence.
ACCESSION   DATAQL010000809 DATAQL010000000
VERSION     DATAQL010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999292"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VHS97_14085"
     CDS             complement(<1..533)
                     /locus_tag="VHS97_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449385.1"
                     /translation="MEEVNLASDPPPISSAAMSRRAEELFEQRRQDLSRTTDRLFSGL
                     LAFEWWAAILTALVIAPRAWSGTTSQIHPFIWAATLLGGAIVSLPVMLAWSQPGRVLT
                     RHLIAVTQMLIGTLMIHLTGGRIETHFYIFGSLAFLAFYRDWKVLVSASVVVAADHVI
                     RGLFWPQSVYGVLLIEP"
     gene            complement(521..1171)
                     /locus_tag="VHS97_14090"
     CDS             complement(521..1171)
                     /locus_tag="VHS97_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449386.1"
                     /translation="MLMILVEMNRLRLATVMATVIWGLTVGIGYLALQLHGASPGQGG
                     RVVELWPDQSRIPLTGGRPTLVIAAHPLCPCTRATIAELTRVLTRCAGKVEVYILILL
                     PERAGHDWGPTDGLRRLGTMPGVHLIDDSGGEEAARFGARTSGLVALYAPDGRLHFRG
                     GITGSRGHEGDNAGQQALLGLIQGNPSSLPCETPVFGCPLFPARSISAESPIPWKK"
     gene            complement(1419..>1996)
                     /locus_tag="VHS97_14095"
     CDS             complement(1419..>1996)
                     /locus_tag="VHS97_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX3449387.1"
                     /translation="FAWCNRQLLAHQAREVFARIFGKSWEQLGMDLVYDVAHNIAKFE
                     EHSVGRGQTKEVCVHRKGATRAFPPGHSDVPAVYASIGQPVIIPGSMGTASWVLVGQA
                     GSMDCSFGTTCHGAGRKLSRTAAVKLAAGRRIDKELDSIGIVARARGHKGLAEEQPAA
                     YKDVDHVVDVVDKIGISKKVARLRPVGVIKG"
BASE COUNT          413 a          623 c          618 g          342 t
ORIGIN      
        1 cacggctcaa tcaggagcac accgtaaact gattggggcc agaagagccc gcgaattacg
       61 tgatcggcgg cgacgacgac cgacgctgag acgaggactt tccagtctcg gtagaaagcg
      121 agaaaggcga gcgacccgaa gatgtagaaa tgggtctcga tccgtccgcc agtgagatgg
      181 atcatcaggg tgcctattag catctgagtc accgcgatca gatgccgggt caagaccctg
      241 ccaggctgag accaggccaa cataaccggt aggctgacaa tcgctccacc gagaagagtg
      301 gctgcccata taaacggatg gatctggctg gtcgtgcccg accaggcgcg tggcgcgatc
      361 acaagcgctg tcagaatcgc cgcccaccac tcgaacgcga gcaaccccga aaacaagcgg
      421 tcggtcgtcc gactgaggtc ctgtcgccgc tgctcgaaaa gctcctcggc acgtcgcgac
      481 atggccgccg atgagatggg gggcggatcg gacgccagat tcacttcttc catgggatgg
      541 gactctcggc agagatggaa cgagccggga agagcggaca gccgaagacg ggagtttcac
      601 agggaagcga cgacggattg ccctggatca ggccaagcag ggcctgctgg ccggcgttat
      661 ccccttcgtg gccacgggaa cccgtgatcc cgccgcggaa atggagccgc ccatctggag
      721 cgtataatgc aacaagaccg gaagtccggg cgccgaagcg ggccgcttcc tcgccgccgg
      781 aatcgtcgat cagatgaaca ccgggcatcg tgccgaggcg gcgcaatccg tccgtcgggc
      841 cccaatcgtg gcctgcacgc tcgggaagca agatgagaat gtagacctcg accttgcccg
      901 cgcaccgggt caatacgcgg gtgagttccg cgatggtggc tcgcgtgcac gggcagagag
      961 gatgagcggc aatcaccaaa gtaggccgcc cgccggtcag gggaatgcga ctctggtcgg
     1021 gccaaagctc gaccactctg ccaccttgac ccggcgaggc gccgtgaagc tgcagggcca
     1081 ggtatccgat acctacggtg agtccccaga taaccgtcgc catcaccgtg gccaaccgta
     1141 ggcgattcat ttctaccaga atcataagca tggccgaccg actcggcccc cttctgtact
     1201 tacatccttt gcgcagttgc agggcgtgtc tcgctttttg cgagattaac ggtcaaagcc
     1261 taggttacga atggggacca tgcaaaatac cgagggcttc aggcgattgc gcacgcccga
     1321 cgccacgaac ccaattcaag agcgtccttc tgcctgcggc tggctgcaga ctcgagcgct
     1381 ccctcctgcc gttcgtgaat aatcgcccta ctgccggctc agcccttgat cactcctacc
     1441 ggacgcaggc gggccacttt tttggaaatc ccgatcttgt cgacgacgtc aaccacgtgg
     1501 tcgacatcct tgtaggccgc cggctgttcc tcggccaagc ctttgtgtcc ccgcgctcgg
     1561 gcgacaatgc cgatcgagtc gagttctttg tcgatccgcc ggccggcggc gagcttgacg
     1621 gccgcggtgc ggctcaactt gcgccccgcg ccatggcagg tcgtcccgaa tgagcagtcc
     1681 atgctgccgg cctggccaac caggacccaa ctggctgtcc ccatactccc cggaatgatc
     1741 acgggctgac cgatggaggc gtagacggcc ggaacgtcgg aatgaccagg cggaaatgca
     1801 cgcgtcgcac ctttgcgatg gacgcagacc tctttggtct ggccgcggcc gacggaatgc
     1861 tcctcgaatt tggcgatgtt gtgggcgacg tcataaacca ggtccatgcc gagctgttcc
     1921 cacgacttgc caaagatccg cgcgaagacc tcacgcgcct ggtgggccag gagttgacgg
     1981 ttgcaccagg cgaagt
//
LOCUS       DATAQL010000810         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_387247, whole genome shotgun sequence.
ACCESSION   DATAQL010000810 DATAQL010000000
VERSION     DATAQL010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387247"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1704
                     /locus_tag="VHS97_14100"
     CDS             <1..>1704
                     /locus_tag="VHS97_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX3449388.1"
                     /translation="QALMWGQAERLVRLSERSSLAYPGGQRKAYGGAVRMGDQTIGWT
                     IWHGKGSGFTHDPVMAGHGRSEAFSSARIRIATLDVAHWSLFRSYQNKQFASRLRCLV
                     LDEAHSYDGVFGANVHYFLNRLYLASEILGRNRPGLFLASATLSSAKKFAAMLLSLNS
                     EDEIVHIADSTRQVIDVVAKTDVPALLKRPRADGLLRIVLLLDGRQGGKPILPFMGSD
                     RELGPDVNAIYFSESKFGSKRMRLELGAKQRARTFVTYDADLPPAERRRLEQELNDSS
                     ARGMTVLATNALELGVDIEGLDVCFIDQVPPGRANLLQRIGRVGRRVDRPGLVLIRLS
                     AEPHDQHLLENPQAAFQLDLNQPRPIPMHLDILRWKHALAAFSEWMYPLSNREIDSSH
                     FSETVQRRFRFKTAPTYQGLRDQFGVLYGSLVDMQDDRGWVYQGFRAGAAKAKIPLME
                     GDREIARIDDVDVFRDAHPEAIFLGHDLNRYRVVNYRGQWSGDAWDEPGTESILGKWL
                     PSLQRVIVERESRLVTTRGSWEEGFNLHQNRTPSHDMDRPRKGTLEFGIWDYTRTWQG
                     YT"
BASE COUNT          349 a          502 c          522 g          331 t
ORIGIN      
        1 caggcgctca tgtgggggca ggccgagcga ttggtccgtc tttccgaacg gagctcgctg
       61 gcctatcccg gcggccagcg caaagcatat ggcggagccg tccggatggg cgaccagacc
      121 attggctgga ccatttggca cggtaagggg agcggcttta cccacgaccc tgtcatggcg
      181 ggtcacggca gatccgaggc attttcatcc gcacggatcc gcatcgcgac cctcgacgtt
      241 gcgcactgga gtctgtttcg cagttatcag aacaaacaat tcgcttcccg attgcggtgc
      301 ctggttctcg acgaagcaca tagctacgac ggcgtctttg gcgccaatgt gcactacttc
      361 ctgaatcgcc tttatctggc gagcgagatc ctggggcgca accgcccggg gctgttcctt
      421 gcctcggcga cactgagctc cgcgaagaaa ttcgccgcaa tgctcctttc gctcaacagc
      481 gaggacgaga tcgttcacat cgcggactcg accaggcaag tcatcgacgt ggtcgccaag
      541 accgacgtgc ctgcactgtt gaaaagaccg cgagcagacg ggttgctgcg gattgtcttg
      601 ctccttgacg gcaggcaggg gggtaagccg atcctgccgt tcatgggatc cgacagggag
      661 ctgggacccg acgtcaacgc catctacttt tccgagagta agtttggcag caaacgaatg
      721 cgactggagc tgggcgccaa acagagggcg cggacctttg tcacatacga cgccgacctt
      781 cctcccgccg aacggcgtag gttggagcaa gagttgaacg actcgtccgc gcggggaatg
      841 acggtattgg cgacgaacgc gctcgagctt ggcgtagata ttgaagggct cgacgtctgc
      901 ttcatcgacc aggttcctcc aggacgggca aacttgctcc agcgcatcgg ccgggtcgga
      961 cggcgcgtcg accggcctgg cctggtcctt atccgtctgt cggccgaacc gcacgaccag
     1021 cacctcctcg aaaaccccca agcggcgttt cagctcgact tgaatcaacc gcggccgatc
     1081 cccatgcatc tggatatcct ccgttggaag catgctctgg cggctttctc cgaatggatg
     1141 tatccgcttt cgaatcggga aatcgactcg tcgcatttca gcgaaactgt tcagcgtcgt
     1201 ttccgattca aaacggcccc cacctatcaa ggactgagag accagttcgg cgtgctgtat
     1261 ggatcgctgg tggacatgca agacgaccgg ggctgggtct atcaaggatt ccgcgccggc
     1321 gcggccaaag caaagatccc cttgatggaa ggcgaccggg aaatagcccg catcgacgac
     1381 gttgacgtct tcagagacgc ccatccggag gcgatctttt tgggacacga cctcaatcga
     1441 taccgcgtgg tcaactaccg gggacagtgg tccggtgacg cgtgggacga gccggggacg
     1501 gaatcgattc tggggaaatg gctgccctcg cttcagagag tgatcgtcga gcgcgaaagc
     1561 cgtctggtaa cgacgcgcgg ctcatgggaa gagggtttca atttgcacca gaatagaacg
     1621 ccttcgcacg acatggatcg ccctcgcaaa gggactcttg agttcgggat ctgggattac
     1681 acccggacct ggcagggata cacg
//
LOCUS       DATAQL010000811         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1915169, whole genome shotgun sequence.
ACCESSION   DATAQL010000811 DATAQL010000000
VERSION     DATAQL010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915169"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..881
                     /locus_tag="VHS97_14105"
     CDS             81..881
                     /locus_tag="VHS97_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3449389.1"
                     /translation="MDFLGSYCLTEPSAGSDAAALKMRAAKDGDSYILNGSKQFISGA
                     GSSDVYVVMARTSDAGAGGISAFVVEKGTPGLNFGANEKKMGWNAQPTRLVVCEDLRV
                     PAANRLGSEGQGFKIAMAGLDGGRLNIGACSLGGAQAALDKAIAYMRERQAFGQKLVD
                     FQALQFKLADMATDLEAARTLLHRAAAALDRKDADATKLCAMAKRFATDIGFRVANDA
                     LQLHGGYGYLAEYGIEKIVRDLRVHQILEGTNEIMRVIVSRKLLDETA"
     gene            878..1810
                     /locus_tag="VHS97_14110"
     CDS             878..1810
                     /locus_tag="VHS97_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3449390.1"
                     /translation="MTDSMFQKYVQKSRQARAPFLFRAPDRFGSVNCLSPSGFHRVAY
                     TDWGPMQAEEVVVCVHGLTRQGRDFDPLAARLADAGYRVICPDLVGRGQSDWLENSLD
                     YVFPQYCADMTSLLAMIGKKMVHWIGTSLGGLIGIVLAGVPRSPIATLTVNDIGPEVA
                     LQASTRVAMRLVSDRWSFDSFEDVFENVRKVYKGCGPLTDEQWLHMARHSVRCDADTE
                     RFIPLTDPKIATAFLWLWYYSMSLWKYWGKLELPVLAVHGDRSDFVTPDLLAKMKRSM
                     PTLSTFKVAETGHMPMLMSCSETDAVLSFLKSCA"
     gene            1935..>3181
                     /locus_tag="VHS97_14115"
     CDS             1935..>3181
                     /locus_tag="VHS97_14115"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3449391.1"
                     /translation="MTAKLAGTPPSAAILPIQMAQDIAASFGRFYAALLREPGTLADA
                     QLDLWRKQVALWQSLARPQEGKARVKDRRFRNSEWESNPMLRGLLQSYLIGAEWLRSL
                     ANKGGIDQTDRKKITFFTDQFIDAIAPTNFPLVNPDVVKKATETGGQNFVRGFSNFLD
                     DALAGSGHVRRADQEAFELGVSIAATKGSVVLRTEMMELIQYEPTTEKVHEVPLLLIP
                     PWVNKYYLFDLQPKTSFIKWAVSEGYTVFAVSWVNPDTSHADKDFENYWLEGPNAAFE
                     AIAKATGQNKVNLVGYCLGGTLTASGLAYMTAKGDERANTATMIATMTDFADPGDFEV
                     FISNENVRALRAQLNGKGYLTDAELSTLFSLLRANDLIWSSAISSYLLAEKAVPSDLL
                     FWFSDGIGMPGKMLDTFMSAIML"
BASE COUNT          639 a          945 c          934 g          663 t
ORIGIN      
        1 tccacaatat gacgacttgg atgatcgacc gcttcggcag cgacgaacag cgggcgaaat
       61 gggttccgcg cctctgcgcg atggatttct tgggcagcta ttgcctaacg gaaccctccg
      121 cggggtcgga cgcggccgct ctcaagatgc gggcggcgaa ggatggcgat tcttacatcc
      181 tcaatggctc gaagcaattc atctccggcg ccggctcgtc tgacgtctac gtcgttatgg
      241 ccagaacctc ggatgcgggg gccggtggca tctcggcctt cgtggtagag aaaggcacgc
      301 ccggcctcaa ctttggcgcg aacgagaaga agatgggctg gaacgcgcaa ccgactcgtc
      361 tcgtcgtctg cgaggatctg cgcgtaccag ccgcgaaccg ccttggctcc gagggccaag
      421 ggttcaagat tgcgatggca gggctcgacg gcggccggct caacatcgga gcttgctcgc
      481 ttggcggcgc ccaggcggcg ctcgacaagg ccattgctta tatgcgcgag cgtcaagcct
      541 tcggccaaaa gctggtcgat ttccaggctc tgcagttcaa gctggccgac atggcgaccg
      601 accttgaagc ggcacgcacg ttgcttcacc gggcagcggc ggcgctcgac cgcaaagatg
      661 ccgacgcgac caagctctgc gccatggcca agcgtttcgc gaccgatatc ggctttaggg
      721 ttgccaacga tgcccttcag ctccatggcg gctacggtta tctcgccgaa tacgggatcg
      781 agaagatcgt ccgggaccta cgcgtacatc agattctgga agggacgaac gagatcatgc
      841 gcgtcatcgt ctcgcgcaag ctcctcgatg agacagcatg accgattcca tgttccaaaa
      901 gtacgtgcag aagtcgcggc aggcacgggc tccattcctg tttcgcgcgc ccgatcgttt
      961 cggctcggtc aactgcttgt ctccgagcgg cttccatcgg gttgcctata cagactgggg
     1021 gccgatgcag gctgaggagg tcgtcgtctg cgtacacggg ctcacgcggc aagggcggga
     1081 tttcgacccc ttggcagcac ggctcgccga cgccgggtac cgcgtcattt gtcccgatct
     1141 cgtcgggcgc ggccagagcg actggcttga gaactctctt gactacgtct tcccgcaata
     1201 ttgcgccgac atgacctcgc tcctggccat gatcggcaag aagatggtgc attggatcgg
     1261 tacctccttg ggcgggctga tcggaatcgt cctggcgggc gtcccgcgta gcccgatcgc
     1321 gacgctgacc gtaaacgata tcgggcccga agtggctctt caggcatcga cacgcgtcgc
     1381 aatgcgactg gtctctgatc gctggtcttt cgactcgttt gaagatgtct ttgagaacgt
     1441 acgaaaggtc tacaaaggct gcggtccgtt gacggatgaa caatggttgc acatggcccg
     1501 acactctgtc aggtgcgatg ccgacactga acgattcatc cccctcaccg atcccaagat
     1561 tgcgacggcc tttctgtggc tctggtacta cagcatgtcg ctctggaaat actggggcaa
     1621 gctcgagctt ccggttctcg cagtgcacgg agatcggtcc gacttcgtga cgccggatct
     1681 cttagccaag atgaaacggt ctatgccgac gctcagcacc ttcaaagttg ccgagacagg
     1741 acacatgccg atgttgatga gctgctccga gacggacgcc gtcctgtctt tcctgaagtc
     1801 ctgcgcctga atcactgttg ccggaggaaa ccatgcccaa gctacccgac gatgtgaaat
     1861 ctgatgcacc gatcaccgcc gatgcgacag ttacaggccc ggcatgggtc cagcttccca
     1921 gcaagatcaa tgccttgacc gccaagctcg cgggcactcc gccctcagcc gccatcctgc
     1981 ccattcagat ggctcaggat attgccgcct ctttcggccg cttctacgcc gcgctgctac
     2041 gcgagcctgg cacgttggct gacgcgcagc tcgatctttg gcgcaaacag gtcgcacttt
     2101 ggcaaagtct ggcccgaccg caggagggca aggcgcgtgt caaggatcgt cgtttccgca
     2161 attccgaatg ggagtcgaac cccatgctgc gagggttgct ccaatcctac ctcattggtg
     2221 ccgagtggtt gcgctccttg gccaataagg gcggcatcga tcagacggat cggaagaaga
     2281 tcactttctt cacagatcag ttcatcgatg cgatagctcc gacaaatttt ccgctcgtca
     2341 atcccgatgt cgtaaagaag gcaacggaga ccggcggaca gaatttcgtc cggggttttt
     2401 cgaacttcct ggacgacgct ctggctggtt ccggccacgt gcggcgcgcg gatcaggagg
     2461 cctttgaact cggagtgtcg atcgctgcca cgaaaggctc ggtggtgcta cgaaccgaga
     2521 tgatggagtt gatccagtat gagccaacaa ccgagaaagt tcacgaggta ccccttcttc
     2581 tgattccgcc gtgggtcaac aaatattacc tgttcgatct ccagccaaaa accagcttca
     2641 tcaaatgggc ggtcagcgag ggctatacgg tcttcgcagt ttcttgggtc aatcccgaca
     2701 cttctcatgc tgacaaggac ttcgagaact attggcttga agggccgaat gccgcctttg
     2761 aggcgatcgc caaggcaacc ggccagaaca aggtgaacct cgtcggctat tgcctcggcg
     2821 gcacgttgac ggcaagtggc ctcgcttaca tgacagcgaa gggcgacgag cgcgccaaca
     2881 ctgcgaccat gatcgccacg atgacggatt tcgccgatcc tggtgatttt gaagtcttca
     2941 tcagcaacga gaacgtccga gccttgcgag cccagctcaa cggtaagggc tatctgacgg
     3001 atgctgaact cagcactctc ttcagcctct tgcgggcgaa cgacctgatc tggtcttcgg
     3061 caatcagttc ctacctcctg gctgagaaag cagtgccgtc cgaccttcta ttctggttct
     3121 cggacgggat tggtatgccg ggcaaaatgc tcgacacctt tatgagcgcg atcatgttgg
     3181 a
//
LOCUS       DATAQL010000812         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_387540, whole genome shotgun sequence.
ACCESSION   DATAQL010000812 DATAQL010000000
VERSION     DATAQL010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387540"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1581
                     /locus_tag="VHS97_14120"
     CDS             196..1581
                     /locus_tag="VHS97_14120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449392.1"
                     /translation="MFDPRASHFWQATIRSGLMDVESLTACWNAIDPKKREDPEHIDR
                     RLARQAVQLKLLTFWQAHQLLAGRTTGYRVDRYLLLSLIGSGGMGRVFLARDTRLGRV
                     VALKILSPERLSNPRAIARFQREARVGAQLQHENLVRIYDFGESDGRYFLVMEYIEGK
                     TIAKVLSEQGPMPPATAVRLGRQVALGLDHAHRKGLIHRDVNPHNILVTHEGTAKLAD
                     LGLAIATDEENPVTRDGATVGTFDYVAPEQARHSRAADIRSDIYSLGCTIYHMCSGQV
                     PFPGHSLPEKLFGHQSLEPTPLYRMVPGLPEGLSEIVRRMMKKSPDERYATPMQVAQA
                     LRQYEGARTGVENGEAKPRIFQPLLVGPAPLLSDVPGDADPALHLLTPDDSYDEITDE
                     VRLILDPDPERSSNEGHSRPESRSSADVSASADLAAGPVAWLPSFWLWGLIVLAVMVT
                     IVLLIIGLGHG"
     gene            complement(1722..2198)
                     /locus_tag="VHS97_14125"
     CDS             complement(1722..2198)
                     /locus_tag="VHS97_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449393.1"
                     /translation="MTQKDLGKALLKLGVTELSGLPDAREQTWRILERDRRRVRLLTG
                     MTIGAWLLSTLLIFTVLVAFGFLFPRVAKLRMDVEQGKVTQDQGEQLRNSHDLGLMKG
                     TLVIAFSVAALTFAAICTVMLNLTTRRATLRQINASLLEISAQLKELRSPPSRSPG"
     gene            complement(2176..2748)
                     /locus_tag="VHS97_14130"
     CDS             complement(2176..2748)
                     /locus_tag="VHS97_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3449394.1"
                     /translation="MNDLTDRLYEQVLVLRCQTGDGAAFGELVERFAARLSYYVRTLL
                     GKERQADAEDVLQDVWLDVHRSIATLTDPGALVAWLYRVARNRTVRHLRKRVTLTRTI
                     DDSHADREIERGEDFSIEDAEAIHRALDGLAPEHREVLVLRFLEEMSYEGIATVTGCP
                     VGTVRSRIHYAKLALRRVLETNDHDAERSR"
     gene            2851..>3422
                     /locus_tag="VHS97_14135"
     CDS             2851..>3422
                     /locus_tag="VHS97_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3449395.1"
                     /translation="MLRPYSLIVIAMVAASSLRGALAQAGDPLVYDVYAVRYAKLRDF
                     AVADLVQGADPGRTLDIAMTVWVLKGPGGRTVLVDAGFYRPPYINGDKVADYTRPDKA
                     LKLLSITPEEITDVVVTHMHWDHADGVDLFPKARVWIQREEYDYYTSKAREPGGDQNS
                     SIPAFVVALVNLNKLGRVNLVDGDAKEIIPG"
BASE COUNT          673 a          997 c         1082 g          670 t
ORIGIN      
        1 ttgattatgc cttgtgatca agaacagggc aagcgcccag gacgaggtgt ttgagcttaa
       61 ataagagttg atttctttag cgcgtttatg gttgggaatg gttgagcgtg gttggcggag
      121 atgttagagt gacctttgat cggcctacat ggtagttcag ggacggtgaa ggcgcagtag
      181 ttctcgggat gccctatgtt tgatccgcgg gcgtcgcatt tttggcaggc gaccatccgt
      241 agcggcctga tggacgtcga gagcctgacc gcctgctgga atgcaatcga cccgaaaaag
      301 cgcgaggatc cagagcacat cgaccgtagg ctggcgcggc aggccgtgca gttgaaactg
      361 ctgactttct ggcaagcgca tcaactactg gcggggcgca cgactgggta ccgggttgat
      421 cgctatctgc ttttgagcct gatcggtagc ggcggcatgg ggcgggtgtt tctggcaagg
      481 gacacacgac tcggccgcgt tgtcgcgctc aagatcctct cgcccgagcg gctcagtaac
      541 ccgcgagcga tcgcgcgatt tcagcgcgag gcccgggtcg gcgcccagtt gcagcatgaa
      601 aacctggttc gcatctacga ctttggcgaa tccgacggcc gatacttcct ggtgatggag
      661 tacatcgagg gtaagacaat cgcgaaagtg ctctcagagc aaggcccaat gccaccggct
      721 accgccgtgc ggctaggccg gcaggtcgcc ttgggactgg accacgctca ccgcaaaggg
      781 ctgattcacc gcgacgtcaa ccctcacaac atcttggtca cgcatgaagg aacggccaag
      841 ctggccgatc tcggcctggc gatcgccaca gacgaggaga atcctgtcac gcgcgatgga
      901 gcgaccgtcg gcacgtttga ctatgtcgct cccgaacagg cgcggcactc gcgcgccgct
      961 gacatccgca gcgatatcta ttcactagga tgtacaatat atcatatgtg cagcggccaa
     1021 gttccgtttc ccggacatag cctgcccgag aagctgttcg ggcaccaatc gctagagccg
     1081 acaccgcttt ataggatggt acctggtctt ccggaaggac tgagcgagat cgtccggcgg
     1141 atgatgaaaa agtcgccaga cgagcggtac gctacgccga tgcaggtcgc tcaggctctg
     1201 aggcaatacg aaggggcccg tacgggcgtc gaaaacggcg aggccaagcc gcgcatattc
     1261 cagccgcttt tggttgggcc ggcgccgctc ttgagtgacg tgccaggcga cgccgatccc
     1321 gcgctccacc tgctcacgcc tgacgactct tatgacgaga ttacagacga ggtccggctg
     1381 attttggacc ccgacccgga acgctcgtcg aacgagggtc attcgcgacc tgagtcgcgg
     1441 tcgtcggcgg acgtctcggc atcggccgac ttggccgcgg ggccggtcgc gtggctgcct
     1501 tcattctggc tctggggatt aatcgtgctg gcggtgatgg tgacgatcgt gctcttgatc
     1561 attggccttg gccacggctg atccatcggg ccgtttcacc ggggcctgca atggatagag
     1621 cgttgctccc gaccccgaag tgatgagtcc ccggcggatt gagcgccggt cgaccgacgt
     1681 gtcccgggga cgataatgag tgactccaga gcatggcggc actaaccggg gcttcgagac
     1741 ggcggcgagc gcagttcttt caactgggcg gagatctcca gtaggctggc gttgatctgc
     1801 cgcaaggtcg cccggcgtgt ggtcaagttc agcatcacgg tacagatcgc cgcgaatgtc
     1861 agcgcggcga cagagaaagc aatcactagc gtacccttca tgaggccgag gtcatgtgaa
     1921 ttccggagtt gctcgccctg atcttgggtg accttgcctt gctcgacatc catgcggagc
     1981 ttggccactc tggggaagag gaacccgaag gccacgagga cggtgaagat taacagcgtc
     2041 gagagcagcc acgcgccgat ggtcatgccg gtgagcaagc gcacacggcg acggtcgcgc
     2101 tccaggatcc tccaagtttg ctcccgggca tcagggaggc cggataattc ggtcacgccg
     2161 agcttcagga gggccttacc gagatctttc tgcgtcatgg tcattcgtct ccagcactcg
     2221 ccgcaaggcg agcttggcgt agtgaattct ggaccgcacc gtcccgacgg gacagccggt
     2281 gacggtcgca atgccttcat aagacatctc ttcgaggaag cgcaggacca gaacctcgcg
     2341 atgctcgggc gccagaccgt caagtgcgcg atggattgcc tcagcgtcct cgatcgagaa
     2401 gtcttccccg cgttcaatct ccctgtccgc gtggctatcg tcgatcgttc gagtcagtgt
     2461 gacccgcttg cggagatggc gaacggtccg gttgcgtgcg acccgataga gccaggccac
     2521 gagggcgccc ggatccgtca gagttgcgat cgatcgatgc acgtccagcc aaacgtcctg
     2581 gagcacgtct tccgcgtccg cttggcgttc cttgccgagg agcgtccgca cgtaataact
     2641 caacctggct gcaaatcgtt cgaccaactc gccgaacgcc gcaccgtcgc cggtctggca
     2701 ccggagaacc agcacttgct catagagtct gtcggtgagg tcgttcatgc gggtcgaccg
     2761 ggagttcatg tggctcgcaa cacccaatag gtcgcgcccc gaggcagaaa agttcaatca
     2821 gtgcgagaaa ctactttcga ggagatttcg atgctgcgtc cttattcatt aatcgtcatc
     2881 gccatggttg cagccagcag cctccgcggc gcgctggcgc aggcgggcga cccactggtc
     2941 tatgacgtgt atgcggtccg ttatgccaag ctccgcgact tcgcggttgc ggacctggta
     3001 cagggtgcgg atcccggacg gacgctcgac atcgcgatga ccgtctgggt gctgaaaggc
     3061 cctggcggca ggacggtcct ggtcgacgcg ggtttctatc ggccgccgta catcaacggc
     3121 gacaaggtcg cggactatac ccgaccggac aaggcgctca agctactgag tatcacgcca
     3181 gaagagatca ccgacgttgt tgtcacccac atgcactggg accacgccga cggcgtcgac
     3241 ctgtttccca aggcgcgcgt gtggattcag agagaggaat acgattatta caccagcaag
     3301 gcccgtgagc ccggcggcga tcagaatagt tccatcccgg ccttcgtcgt ggcacttgtg
     3361 aacctcaaca agctggggcg tgttaatctg gtcgatggag acgccaagga gatcattccg
     3421 gg
//
LOCUS       DATAQL010000813         5145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_694648, whole genome shotgun sequence.
ACCESSION   DATAQL010000813 DATAQL010000000
VERSION     DATAQL010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694648"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VHS97_14140"
     CDS             complement(<1..389)
                     /locus_tag="VHS97_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449396.1"
                     /translation="MTAYDPTDRYKVQQIQGWSVLVNKSFLETQPALAKQTLTLMRAQ
                     LRQIVRGVPAEAVEKLRTIRIWVEEKEPHHPCMTYHPNIVWLKENGMNPDKARCVEVA
                     NARNFLEWIRQQPWMVLHELAHGYHHQ"
     gene            complement(576..4142)
                     /locus_tag="VHS97_14145"
     CDS             complement(576..4142)
                     /locus_tag="VHS97_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449397.1"
                     /translation="MISRFFIDRPVFAVVLSVVIVLAGAVAVFTLPVAQYPEVTPPTV
                     QVTAIYPGANALTVRNTVAAPIEEQVSGVENMLYMSSQCTNDGAYNLTVTFRLGMDTD
                     MAQVLVQNRVSLALPVIPLLVQNEGISVKKMSPNTLMIVNLLSPDGRYDSTFLSNYAT
                     IYIKDELGRLPGVAGITYLGQRDYSLRAWLDPAKLAALNLSATDVVTAISQQNLQVAA
                     GQIGQQPVPRGQQFQLTINTLGRLVEPEQFGAIILKAGGNVKPGMQSSSGGSGAQSAQ
                     GQGGQASAGFQGGPTDEGGSNSQGAPMSQASGIVRLRDVARVELGSRQYDQSCTLDGR
                     PSVAVSIYQLPGSNALETADGVYAKMKELRSRFPDGLDYKIVYDTTPFIRESVNEVFN
                     TLRDAVILVGIVVLVFLQDWKAMILPMIDVPVSLIGTFAIMAAMGFSLNNLTLFGLVL
                     AIGIVVDDAIVVLENIERLIATGLDSRTATIQAMEEITGPILAITLVLSSVFIPCCFL
                     GGITGQFFRQFAVTIAVSTIISAINALTMTPSRAVMIFKTEEGGHGHELKREALPWWI
                     FAIVGGMLTIWLGQDVFAGKYGLPPARNPAEDAEVSTLMSWGITGLYFAPGALIGGLF
                     GLLFIRPINAVLGWLFRAFNRLFDQMTVVYGRIVGHVLRISTIVLIAYGGLLVLTYWQ
                     FTHAPTGFVPQQDKGYLLLNVQLPDSASVERTQRVMARIETLARETPGVQHTVGVSGQ
                     SLLLNANAPNLGSMYIMLKEFSHRRGSKLTGDAIAAELKEKCQREIRGAIVTAFGAPP
                     IDGLGTTGGFKLIIEDRGNLGFADLQRISDRIVERGNNTPGLQGLFNSSRANTPWLYL
                     DIDRTKCVALGVPISELFNTLQVYLGSYYVNNFNEFGRSWQVNIQADKSFRGDARDIG
                     QLQVRNNQGQMIKLATLMDVRNTSGPIMGMRYNMYSSTAITGNPAPGTSSGEAIAQMQ
                     QIAQEELPRSMAFDWTELAFMQLQAGNTATGVFGLAVVFVFLVLAAQYESWKLPLAVI
                     LVVPMCLMCSLVGVSLAGMDVSLFTQIGFVVLVGLASKNAILIVEFAKQSQESGVPRR
                     EATLAACRLRLRPILMTSFAFIFGVVPLVIAEGAGAEMRRSLGTAVFSGMLGVTLFGI
                     FLTPVFYYVIQRFGGGEKTDASHQQKTDLAPEREELAAALSE"
     gene            complement(4139..>5145)
                     /locus_tag="VHS97_14150"
     CDS             complement(4139..>5145)
                     /locus_tag="VHS97_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449398.1"
                     /translation="KWGQMVPMGTLVNVRDFPGPIVVTRYNLYTAAAITGNMGAGTSS
                     GDAINTVNKTKDETLPISMTGEWTELMYVQIRAGNTAIFVFLLAVAAVFLALAALYES
                     WSLPLAVILVVPMCLLCSVSGVLYTGREVNIFVQIGLVVLVGLACKNAILIVEYAKQK
                     HLEGLPRYAATLEACQLRLRPILMTSFAFIIGVIPLVVASGAGSEMRRSLGIAVFSGM
                     VGVTLFGIFLTPVFFSVILGFSETSLFASAAVRWVGSAVVSGLAGSGMGFLFARLARA
                     GVGRMYGAIGLGGSAGVLAALVALGIHERIRFQSAASRQAHSPPPHLKLGAQTGEHKS
                     "
BASE COUNT         1091 a         1589 c         1514 g          951 t
ORIGIN      
        1 aattgatgat gatatccgtg cgccagctca tggagcacca tccagggctg ttgccggatc
       61 cattcgagga agttgcgcgc attggccact tcgacgcaac gagccttgtc gggattcatg
      121 ccgttttctt tgagccagac gatgttcgga tgataagtca tgcagggatg gtgcggctct
      181 ttttcctcga cccagattcg aatggtgcgc agcttctcga cggcctcggc tggaacgccc
      241 cggacgatct ggcgaagctg cgctcgcatg agggtgagtg tttgtttagc gagcgcgggc
      301 tgtgtttcca agaagctctt gttcacgagt acggaccagc cctggatctg ctggacctta
      361 tatcgatccg ttggatcgta ggcggtcatc gcggcggcct tctgcacgcg agacttacgt
      421 tcggcagcct catactgttt tgcctgtttt gccgccaaag cgctatctgt gaaaaaaatt
      481 gtgacagcga atgccaagcc tgcctgtatc agacatgtcc aatcacaagc ccgccattct
      541 gccatggtgc aagctttcca tgaaaacgag gagacctact ccgacagcgc cgccgccagc
      601 tcttcgcgct cgggcgccaa atccgtcttc tgctgatggc ttgcgtccgt cttttcgccg
      661 ccaccgaaac gctggatgac gtagtagaag acaggagtaa ggaaaatgcc gaaaagagtc
      721 acgccgagca tcccgctgaa taccgcagtt cccagtgatc gtcgcatctc ggcgccggct
      781 ccttcggcga tcacgagcgg tacgacgccg aagatgaagg caaatgatgt catcaggatc
      841 ggccgcagcc ggagccggca ggccgcgagc gtggcttcac gccggggtac gccggactct
      901 tggctctgct tggcgaactc gacgatcaga atcgcgtttt tgctggccag gccaaccaaa
      961 acgacgaagc cgatctgggt gaaaaggctg acatccatgc cggccaagct tacaccgacg
     1021 agtgagcaca taagacacat cggcaccacc aggatgacgg ccagcggcag cttccagctc
     1081 tcgtactggg cggccagcac caggaacaca aagaccaccg ccagcccgaa aactccggtt
     1141 gccgtgttgc ccgcctgcaa ttgcataaaa gccagctcgg tccagtcgaa agccatcgac
     1201 cgcggcaatt cttcttgcgc aatctgctgc atttgcgcaa tggcctcccc ggagcttgtc
     1261 cccggtgccg ggttgcccgt gatcgccgtg gaagagtaca tattatagcg catgcccatg
     1321 atcgggccgc tcgtgtttct cacgtccatg agcgtggcca gcttgatcat ctggccctgg
     1381 ttattccgta cctgaagctg cccgatgtcg cgggcatcgc cgcgaaagct tttatcggcc
     1441 tggatattca cctgccacga gcggccgaac tcgttgaaat tattgacata gtacgaaccc
     1501 aggtagacct ggagcgtgtt gaaaagctcg ctaatcggca caccaagagc cacacatttg
     1561 gtccggtcga tatcgaggta cagccaggga gtattggcgc gcgagctatt gaagagcccc
     1621 tgcaagccag gcgtgttgtt gccgcgctcc acaatccggt cgctgatccg ctgaaggtct
     1681 gcaaacccga gattgccgcg atcttcgatg atgagcttga agccgccggt cgttcccaac
     1741 ccgtcgatcg gcggggctcc aaacgccgtc acaatcgcgc cgcgaatctc gcgctgacat
     1801 ttttctttca gctccgcggc gatcgcgtca cccgtgagct tgcttccgcg ccgatgcgaa
     1861 aattccttga gcatgatgta catcgagccg agattgggcg cgttggcgtt caagagcagc
     1921 gactggcctg aaacgccgac cgtatgctga accccgggcg tttcgcgggc cagggtctcg
     1981 atgcgagcca tgacgcgctg tgtccgttcc accgaggcgg agtcgggcaa ctgcacgttc
     2041 aagagcaggt accccttgtc ttgttggggg acaaagcccg tcggcgcatg ggtgaattgc
     2101 caataagtga gcaccaacag accaccgtac gcaatgagca cgatcgtact gatgcgcagg
     2161 acgtgaccaa cgattcggcc gtaaaccacg gtcatctggt cgaagaggcg gttgaaggcg
     2221 cggaagagcc accccaggac tgcgttaatc ggccggatga ataagaggcc gaacagcccc
     2281 ccgatcaacg ccccgggcgc aaaatatagg ccggtgattc cccaggacat caaggtcgaa
     2341 acctcggcat cttcggctgg gttccgtgcg ggcggcaggc catacttgcc ggcaaagacg
     2401 tcttggccca gccagatggt caacatccct ccgacgatgg caaaaatcca ccagggcaat
     2461 gcctcgcgtt tgagctcgtg accatggccc ccctcttcgg tcttgaaaat catcaccgca
     2521 cgcgacggcg tcatggtcag ggcattgatg gccgagatga tcgtcgaaac ggcaatcgtc
     2581 accgcaaact ggcgaaagaa ctggccggtg attcccccca agaagcagca cggtatgaac
     2641 accgagctga gcacgagcgt gatcgccaga atcgggccgg tgatctcttc catggcctgg
     2701 atcgtggcgg tgcgcgagtc caggccggtc gcgatcaggc gctcgatgtt ttccagcacg
     2761 acgatcgcgt cgtcgaccac gatgccaatg gccagcacga gaccgaagag cgtcaggttg
     2821 ttcagactga aacccatggc cgccatgatc gcgaacgtgc cgatcaacga aaccggcacg
     2881 tcgatcatcg gcaggatcat ggccttccag tcctgcaaga acaccagcac cacgatgccg
     2941 accaaaatca ccgcatcgcg taaggtattg aagacctcgt tgaccgactc gcgaataaac
     3001 ggcgtcgtgt cgtaaacaat cttgtaatcg agcccatccg ggaagcggga ccgcagctcc
     3061 ttcatcttgg cgtagacgcc atcggccgtt tcgagcgcat tggacccagg gagctgatag
     3121 atcgagaccg ccacggaggg acgaccgtcc aaggtacagg actgatcgta ctgccgcgag
     3181 cccagctcga cacgggccac atcgcgcagc cggacgatcc cgctggcctg cgacatgggt
     3241 gcgccttgac tgttggaccc tccttcgtca gtcgggcctc cttgaaatcc ggcgctggcc
     3301 tgtccgccct gaccctgggc tgactgagca ccggagcccc ctgacgacga ctgcattccc
     3361 ggttttacat tgccccccgc cttgagaatg atcgcgccga attgttccgg ttcgaccagc
     3421 cggcccaagg tgttgatcgt gagctgaaat tgctgacccc gcgggaccgg ttgctggccg
     3481 atctggccgg ccgcgacctg caggttttgc tggctgatgg cggtgaccac gtcggtggcg
     3541 ctcagattga gcgctgccag cttcgcgggg tcgagccagg cccgcagact gtaatcgcgc
     3601 tggcccaggt aggtgatgcc ggcaacacct gggagccgac ccagttcgtc cttgatgtaa
     3661 atcgtggcgt agttactcag aaacgtgctg tcgtagcgac cgtcgggcga caacaagttg
     3721 acgatcatca acgtattcgg cgacatcttc ttgacgctga tcccctcgtt ctgaaccaaa
     3781 agcgggatga ccggcagcgc cagcgacacg cggttttgca ccaagacctg ggccatgtcg
     3841 gtgtccatgc ccagccggaa ggtgacggtc aagttgtagg cgccgtcgtt ggtacactgc
     3901 gacgacatgt acaacatgtt ctcgacgccg ctcacctgct cttcgatcgg ggccgccacg
     3961 gtgttccgca cggtcagggc gttggcgcca gggtagatcg cggtcacctg cacggtggga
     4021 ggcgttacct cgggatactg agccaccggg agcgtgaaca cggccacagc gcccgccagg
     4081 acgatgacga ccgagagcac gacggcgaag accggccggt cgatgaagaa gcgagaaatc
     4141 acgacttatg ctctcctgtt tgggcaccga gtttcaggtg gggaggtgga gaatgagcct
     4201 ggcgcgaagc cgctgactga aagcggatcc gctcatgaat ccccagggca accagcgcgg
     4261 ccaggactcc cgcgctgcca ccgagcccaa tcgccccgta cattcgaccg acgcccgccc
     4321 ttgcgagtct tgcaaacaga aagcccatcc ccgacccggc cagaccgctc accacggcgg
     4381 agccgaccca gcgcacggcc gcggaggcga acagcgacgt ttcactgaac cccagaatga
     4441 cagagaagaa gaccggcgtg aggaaaatcc cgaacaacgt cacacccacc atgccactga
     4501 agacggcaat ccccagcgac cgccgcatct cggagccggc gccggaggcc acgaccagcg
     4561 ggatcacgcc gatgatgaaa gcgaacgagg tcatcaggat cggccgcagt ctaagttggc
     4621 acgcctcgag cgtcgccgcg taacgcggca gtccctccag gtgcttttgc ttggcgtact
     4681 cgactatcag gatcgcgttc ttgcacgcga gccccaccag gaccacaagg ccgatctgca
     4741 cgaagatgtt aacttcgcgg cctgtgtaga gcactccgga caccgaacag agcaggcaca
     4801 tcggcaccac cagaatgacc gccagtggta gcgaccagct ctcgtaaagc gccgcgagtg
     4861 ccaggaagac ggctgcgacc gccagcagga atacaaagat cgccgtattt cccgcccgga
     4921 tctgcacgta catcaactcg gtccactcac ccgtcatgga aatgggcagg gtctcatcct
     4981 tcgttttgtt gacggtgttg atcgcgtcac cggagctggt cccggcaccc atattcccgg
     5041 tgatagccgc ggcggtgtag agattgtaac gcgtcacgac gatgggaccg gggaaatcgc
     5101 ggacattgac cagcgtaccc atcggcacca tttggcccca cttgt
//
LOCUS       DATAQL010000814         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_694834, whole genome shotgun sequence.
ACCESSION   DATAQL010000814 DATAQL010000000
VERSION     DATAQL010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694834"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VHS97_14155"
     CDS             complement(<1..271)
                     /locus_tag="VHS97_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3449399.1"
                     /translation="MIELKGVSKTYSGLVVLHQIDLEIAAGRTTVLIGPSGCGKSTLL
                     RVMIGLVQPDAGYVAIAGTQLNPATILEVRHGLGYVIQDGGLFPHL"
     gene            484..1263
                     /locus_tag="VHS97_14160"
     CDS             484..1263
                     /locus_tag="VHS97_14160"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3449400.1"
                     /translation="MKLDALKVGELAKRTGLTVRTLHHYDEIGLLRPSLHTEAGHRLY
                     TLRDLARLQQIVSLRQLGFSLDQVRECLERPDFSPLEVIGMHAAHLREQIGLQQRLCE
                     RLDSIAAHLRAAGDVSADEFLKTIEGITMIESYYTPEQMEYLKKRRELVGDDRIKQVE
                     KEWPELIAQVRAEMQKGTAPTAPAVLELARRWTGLVNEFTGGDQGIERSVGRLWKEQG
                     DNLVARHGAEYDSRDVFEYIGQAIAVLKGSERATEDGVSPP"
     gene            complement(1250..1816)
                     /locus_tag="VHS97_14165"
     CDS             complement(1250..1816)
                     /locus_tag="VHS97_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449401.1"
                     /translation="MDAKEFVQLSMLAMGSEIQGKTKLQKTVYFLGLMTGCLDDLGYR
                     AHFYGPYSDEVADAVGWLNTIGAVDQSTSGAGTVDNSGFEIRRYDYRLNPQGRRFAKA
                     TSLRHEDLWRKLQHAVDLLKRSGDLDYVQMSIAAKTYFMLKETAGQASLKDLARLATR
                     FGWDVTPQQVQEAARYLELLGLAQITVD"
     gene            complement(1816..>2056)
                     /locus_tag="VHS97_14170"
     CDS             complement(1816..>2056)
                     /locus_tag="VHS97_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449402.1"
                     /translation="NDESGIPIATRGAPRFFQDESALFASINESYTEGFVEVYAPAPL
                     TWADRTQRSKWLTSLKAPIRESIESIVGRTLKGTS"
BASE COUNT          433 a          588 c          618 g          417 t
ORIGIN      
        1 tcaagtgggg aaaaagaccg ccatcttgga tgacataacc gagtccatgc cgcacctcca
       61 ggatggtcgc cgggttgagc tgggttcccg cgatggcgac gtagcccgca tcaggctgga
      121 ccaagccgat catgacacga agcagcgtcg acttgccgca accgctcgga ccgatcaaga
      181 ccgtcgtccg tcccgctgcg atctccaggt cgatctggtg gagcacgacc aacccgctgt
      241 atgtcttcga gacgcccttc agctcgatca tccacgatgt tcccggtaat ttgatcgcgt
      301 gaaccagaat tcattttccc atgccgacag tggaagggga agcgcctcac ccgcaacggg
      361 gtgcccctcc gtaattcaaa taattgtatt cttcgcgaaa aaggtcttga tcctcacgtt
      421 acgtgaggtc gtaacattcg gttgtacccc cagttcccgc ggacggtggc aaagaaattc
      481 gtcatgaaac tcgacgcatt gaaagtcggt gagctcgcaa agcggacggg cctgactgtc
      541 cgcacgctcc accattacga cgagatcggg ctcctgaggc cgtcgctgca taccgaagcc
      601 ggacataggc tttacacgct ccgcgatctt gcgcgcttgc agcagattgt ctcgctgcga
      661 cagctcggat tctcgctcga ccaggtccgc gaatgcctgg aacggccgga tttttcgccg
      721 ctggaggtga tcggtatgca cgccgcacat ctgcgggaac agatcgggct acagcaacgg
      781 ctctgtgagc ggctcgactc gattgccgct catctacgtg cggcggggga tgtctccgcc
      841 gatgagtttc tcaagacgat cgaggggata accatgatcg agagttatta cacaccagag
      901 caaatggagt atctgaaaaa gcgccgggag ctggtcggtg acgaccgcat caagcaggtg
      961 gagaaagaat ggcccgagct gatcgcccag gtccgggccg agatgcagaa gggaaccgct
     1021 ccgacggctc cggccgtgct cgagctcgcc aggcgctgga cgggcctcgt caatgagttt
     1081 accggcggcg accaaggcat cgaacgatcc gtcggccgat tatggaagga acaaggcgac
     1141 aacctggtcg cccggcatgg cgcagagtac gattcgcgcg acgtgttcga gtacatcggt
     1201 caggcaatag cggtcttgaa gggctccgaa cgcgcgacgg aagacggcgt cagtccaccg
     1261 tgatttgtgc taagccaaga agttcgaggt aacgtgccgc ttcctggacc tgctgcggag
     1321 taacgtccca cccgaacctg gtagcaagcc gggccagatc ttttaggctg gcttgtccag
     1381 cggtttcttt gagcatgaaa taggttttgg cggcgatgga catttgaacg tagtcgagat
     1441 cgccggacct cttcaggaga tcgaccgcgt gttgaagctt cctccagagg tcttcgtgtc
     1501 gaagggacgt tgcctttgcg aaccgtctgc cttgcgggtt cagacgatag tcgtaccgac
     1561 ggatctcgaa ccctgagttg tccaccgtgc cggcgccgga ggtcgactgg tcgacggcgc
     1621 cgatggtatt cagccagcca accgcgtcgg caacttcatc cgagtacgga ccgtaaaagt
     1681 gcgcacgata accgaggtcg tcgaggcatc ccgtcatcaa gccgaggaag tagacggtct
     1741 tctggagctt agtcttgccc tgaatctcgg aacccattgc gagcattgaa agttgcacga
     1801 attcttttgc atccattatg acgttccttt aagggtccgg ccgacgatcg actcgatcga
     1861 ctcacgaatt ggcgctttga gagaggtcag ccacttcgaa cgctgtgttc ggtccgccca
     1921 ggtcagcggg gcgggggcgt atacctcgac gaatccctcc gtgtaacttt cgttgatcga
     1981 cgcaaagagg gcggactcgt cctggaagaa ccgcggcgcc ccacgggtcg cgattggaat
     2041 tccggattcg tcgttg
//
LOCUS       DATAQL010000815         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4206541, whole genome shotgun sequence.
ACCESSION   DATAQL010000815 DATAQL010000000
VERSION     DATAQL010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4206541"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VHS97_14175"
     CDS             complement(<1..545)
                     /locus_tag="VHS97_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449403.1"
                     /translation="MNVRSRKKIVVLGMMAKLPVAGVVWQTMHYLVGFQRLGYDVYYV
                     EAHATAPTMFLDSIDHDGSAKVATFLDRVLRRFDLGDRWAFHALHSDRRSYGLSPWQL
                     ASLYDSARWIINLHGGTLPMPEHRATNRLIFLETDPVQVQIELSEDNPQTIEFLDSHC
                     AYFTFGENYGKPNCGLPVSGR"
     gene            complement(542..>2612)
                     /locus_tag="VHS97_14180"
     CDS             complement(542..>2612)
                     /locus_tag="VHS97_14180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3449404.1"
                     /translation="NLVGDRPLVLGETGMDTLRHGEAQQARMLAGHLCEAELMGLAGA
                     YVFSWTDDWHTGGNPIEDWAFGITYADRKPKESFVAVQQVFKAALPQVLEETPRVSVV
                     VCSYNGGATLDECLRSLSTLNYPNYEVIIVDDGSTDNTQQILARFPAVRAIYQTHSGL
                     SVARNAGLQSGTGALIAYTDSDCIADPDWLIHLVHQLIRSGAAAVGGPNLTPEDGWMA
                     ACVAAAPGQPTHVLVSDQVAEHIPGCNMAFRREALEAINGFDPQFLKAGDDVDICWRL
                     QHEGLSITFAPGAFVWHHRRQNPRSYLRQQAGYGTAEALLHFKHPDKFNGRGDGKWGG
                     VMYGTSFRGLQVASPIIYRGTFGTGMFQCLYQPGAAHWLMAPSTFEWHVAALLIGLAG
                     AIVRPWGWVIGAVMLGLSLLVAALQAAQADLNPAHRRRYSRLVIATLCYAQPLVRSWA
                     RYWTRLSSQRAPGSGLAPPDVPREWLSLTGCRSEGYWSEAGIDRTEILRRVVAFMDEH
                     RWGKVLDTGWFEWDMAVFCDWGLILKVVTVQEEHGQGKRLIRIRFRLGPTARMKVLVV
                     VCLAALAVLVVPYPWVASAAAALMVGLGARAWGRGLAAATRVIALFRAQAYQLGLIVC
                     PNRSEPRLTSAFLRDGSLRAAGSLEDTQPPPNPAGAGGPNAVVPVFHSVVALESPSAS
                     EIEEQFA"
BASE COUNT          526 a          832 c          812 g          442 t
ORIGIN      
        1 tagcggcccg acaccgggag accgcaatta ggcttgccgt aattctctcc aaaggtgaag
       61 taagcgcaat gagaatcgag gaattcgatg gtctgcggat tgtcttccga cagttcgatc
      121 tgaacctgga ccgggtccgt ttccaggaag atcagccggt tggtcgcgcg atgctcgggc
      181 atcggcaggg tcccgccatg gaggttaatg atccatcggg ccgagtcgta aaggctcgca
      241 agttgccacg gactcaggcc gtagctccgt ctgtcggaat gcaacgcgtg gaacgcccag
      301 cggtccccca ggtcgaatcg gcgcaaaacg cgatcgagga aagttgcgac cttggccgac
      361 ccgtcgtggt ctattgagtc caggaacatc gtgggtgcgg tggcgtgcgc ctccacgtaa
      421 tacacgtcgt agccgaggcg ctggaagccg accaggtaat gcatcgtctg ccagaccacg
      481 ccggcgacag gcaacttggc catcatgccg agcacaacga ttttctttcg ggaccgaacg
      541 ttcaagcaaa ttgctcctca atctcggacg ccgacgggct ttcaagcgcc accacggaat
      601 gaaagacagg tacaaccgca tttggtccac ccgcccccgc cggattcgga ggaggctggg
      661 tgtcttctag ggaaccggcc gcacgtaggg acccgtcgcg gagaaatgcc gacgtcaagc
      721 gtggctcact cctattggga cagacgatca agcccagttg gtacgcctgg gcgcggaaga
      781 gcgcgatcac acgggtcgcc gcggccaacc cgcggcccca ggctcgggcg ccgaggccga
      841 ccatcagcgc cgcggcggcg ctcgcgaccc aaggataggg cacgaccagg accgcgaggg
      901 cggcgaggca aaccacgacg agcaccttca tcctggccgt tgggcccaaa cgaaagcgga
      961 tgcggatcag ccgtttgcct tggccgtgtt cctcctggac cgtcacgact ttcaggatta
     1021 atccccagtc gcaaaagacc gccatgtccc actcgaacca gcctgtgtcg aggaccttgc
     1081 cccagcgatg ttcgtccata aaggcgacca cccgcctcag aatctcggta cgatcgatgc
     1141 ccgcttccga ccagtagcct tccgaacggc agcccgtcaa cgataaccac tctcgcggga
     1201 cgtccggcgg cgccagacca gacccggggg cacgttgcga ggacagccgg gtccaatacc
     1261 gcgcccacga ccggacgagc ggttgggcat aacagagcgt ggcgatgacg agtcgggagt
     1321 acctgcgccg gtgggccggg ttaaggtcgg cctgcgccgc ttgcaacgcg gcgaccagca
     1381 gcgacaggcc cagcataacc gccccgatga cccatcccca cggacggacg atcgcccccg
     1441 ccaggccgat cagcaaggca gctacgtgcc actcgaatgt gctgggggcc atcagccaat
     1501 gagcggctcc cggctgatag aggcactgga acatgccggt gccaaacgtg ccgcgataaa
     1561 taatagggga ggcgacctga agaccccgga acgaggtgcc atacatcacc cctccccact
     1621 taccgtcacc gcggccgttg aacttatctg ggtgcttaaa gtggagcagg gcttcggccg
     1681 tgccataacc ggcctgttgt cttaggtacg accgcgggtt ctggcggcga tggtgccaga
     1741 cgaacgcccc cggcgcgaac gtgattgaaa ggccttcatg ttgaagacgc cagcatatgt
     1801 ccacgtcgtc gccggccttg agaaactggg ggtcaaagcc gttgatcgcc tcgagcgcct
     1861 cccggcggaa cgccatattg cagcctggga tgtgctcggc gacctggtcg cttaccagga
     1921 cgtgggtcgg ctgccctggg gcggccgcga cgcacgccgc catccagcca tcttcgggcg
     1981 tcaggtttgg cccccccacc gccgcggcac cgcttcgaat cagttgatgg accaggtgga
     2041 tcagccagtc cggatcggca atacagtcgg aatcggtgta cgcaatcagc gcacccgtgc
     2101 cggactgaag gccggcgttt cgcgcgacac ttaagccaga gtgcgtttgg tatatggctc
     2161 gtaccgcggg gaacctcgcc agaatctgct gcgtattgtc ggtcgagcca tcatccacga
     2221 tgatcacttc gtagttcgga taattgaggg tcgagagcga tcggaggcac tcgtcgagag
     2281 ttgctccgcc gttatacgag cacacgacca ccgagactcg cggtgtctcc tcgaggactt
     2341 ggggaagggc tgccttgaag acctgctgca ctgcaacaaa ggactccttc ggcttgcggt
     2401 cggcgtaggt gatgccgaat gcccaatcct cgatcgggtt gccgcccgtg tgccagtcgt
     2461 cagtccagga aaaaacatag gctccggcca ggcccatcag ctcggcctcg cacaagtggc
     2521 ccgcgagcat gcgggcttgc tgcgcctcgc cgtgccgaag cgtgtccatg ccggtctccc
     2581 ccaataccaa gggacgatcg ccaaccaggt tt
//
LOCUS       DATAQL010000816         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4206677, whole genome shotgun sequence.
ACCESSION   DATAQL010000816 DATAQL010000000
VERSION     DATAQL010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4206677"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1046..>1603
                     /locus_tag="VHS97_14185"
     CDS             1046..>1603
                     /locus_tag="VHS97_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3449405.1"
                     /translation="MKFGIFYEHQLPRPWEPGAELRLFQHALDQVELADRLGIDYAWE
                     VEHHFLEEYSHSSAPEVFLAAASQRSRRIRLGHGIVLMPPGYNPPARVAERVATLDLV
                     SGGRVDWGTGESASRAELEGFGVNPAERRAMWRETVEQVANMMVMDPYPGFAGKYFAM
                     PARNVLPKPVQKPHPPIWVACSNRET"
BASE COUNT          287 a          490 c          513 g          313 t
ORIGIN      
        1 acagggctct gggcagcgcc atttccggcg aatgcggcta tgggtggccc agccgggtaa
       61 agtgtgaggg tcacggcaaa cctctatttc ggggggtttc ggttaccacc cggttttgcg
      121 aacggcccga aggaactcta caaatgacgc gtcaggatcg taggcatccg gcgcgaacca
      181 ctgcggcggc gcgttctgcc aggacccggt cggatacgag gctgcgctag ggcgcagcgg
      241 gcctaaccgg caagtaccat tagtgcgcgg cagacggccg gcgccgaggc gaggtcaggc
      301 aggacgtagc gcattcccgc cggtagtcgg ttcgacgtca ctgggaatgt cgtcttcacc
      361 actgagccgg ggctcaggat caggatcaga attgtcaagt gctcgtctct ctcggtgagt
      421 cctcggggta acggcagcgg tccgggagcg cccgctttac cccaacaaac cgaccacccg
      481 ctccctggga tcaattatgt attcagtgcc aaacggcgtc ctgcgagttc ggccttctag
      541 cgaccccgcg acaggagtgc atcgaaagac gacgttccct gctcgttagg ttgttttact
      601 cgccatgctc taggttctca gatccgtgcg ggccatcgtc tcgctttttg gcgctcctcg
      661 gcaaagaatt tcgtcggcga tgggacagtg ccggcggccc aagcaatgat tttccggcct
      721 tgacggctcg cgacgctgga gtgtgaaagc gacccctttc caccttgagc cacaaattca
      781 cccgcacaaa tcaactcgcc ggggaacgat cgagattcga actctcgatc ccgcaaaatg
      841 gccccgatat gctgctcgat gcgggtcgtc gtattctatc atcgcggcat gggtgaagtt
      901 gcagttcgat ccgatatttt tcctctggtc gacggcggcc tgatcggtgg gcgggctttg
      961 gtcagaggtg gagcagcgac gcgcaatatt gcgcagcctc tttggcgtgc cgcccacgta
     1021 gggcggggcc ggtgaaggag cgaccatgaa attcgggatc ttctatgagc accagctgcc
     1081 gcgtccctgg gagcccggcg ccgagctccg cctcttccag cacgcgctgg atcaggtcga
     1141 gctggcggac cgcctgggca ttgactatgc ctgggaggtg gagcaccact tcctcgagga
     1201 atactcgcat tcgtcggccc cggaggtgtt cctcgccgcc gcgtcccagc gttcgcggcg
     1261 catccgcctc ggacatggca tcgtcttgat gccgccagga tacaaccccc ccgcgcgcgt
     1321 cgctgagcgt gtggcgaccc tcgatttagt ctcgggcggc cgagtcgact gggggacggg
     1381 ggaatccgca tcacgggcgg aactcgaagg gtttggtgta aacccggctg agcgtcgtgc
     1441 gatgtggcgg gagaccgtcg agcaggtcgc caatatgatg gtgatggacc cctacccggg
     1501 cttcgccggc aaatacttcg cgatgcccgc gcgcaacgtg ctccccaagc cagtacaaaa
     1561 gccccacccg ccgatttggg tggcctgttc gaatagggag acc
//
LOCUS       DATAQL010000817         5833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4968787, whole genome shotgun sequence.
ACCESSION   DATAQL010000817 DATAQL010000000
VERSION     DATAQL010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4968787"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            571..1620
                     /gene="aroF"
                     /locus_tag="VHS97_14190"
     CDS             571..1620
                     /gene="aroF"
                     /locus_tag="VHS97_14190"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX3449406.1"
                     /translation="MIIVLKPHSTPEVIEHVLERINALGFTPHLSQGVARTIIGVIGD
                     ETKLQVEPLQAIPGVEQVVPIMKPFKLASREFHPEPSVFESKGVKIGGGNLAVIAGPC
                     AIESESLLFEIAARVRDAGANILRGGAFKPRTSPYSFQGLGLDGLKILQAAGDRFEMP
                     VVTEVMDPRQVELVERYTDILQVGARNMQNFDLLKECGRTRVPILLKRGMSASVKDLL
                     MSAEYVLSEGNRQVILCERGIRTFEESTRNTLDLSIVPNVQGQSHLPIIVDPSHATGR
                     PDLIPAMARAAIAGGADGIHIEVHSCPERALSDGPQALVPDQFDRLMDEIHQLAELLG
                     KSVDSCKGEPVTCAP"
     gene            1608..2390
                     /gene="tpiA"
                     /locus_tag="VHS97_14195"
     CDS             1608..2390
                     /gene="tpiA"
                     /locus_tag="VHS97_14195"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX3449407.1"
                     /translation="MRTLMIAGNWKMNPATHVEAVALADAVKAGVGQETSVRVALCPP
                     AVFLSQVDAALAGTPIGLGAQNMHWETSGAHTGEISGAMLVDIGCTHVILGHSERRHG
                     MGETDTVVNAKLRAALTIGLIPIVCLGETKDQRLADQTEEVLLAQLTGSLAGVTPEQM
                     AGTILAYEPVWAIGTGLTATPEQAQAAHHFIRSWLKGHFGEATAARVIVQYGGSVKPD
                     NAAELLACPDIDGALVGGASLKAGDFLAIFRGGLDVTPRKSA"
     gene            2416..2886
                     /gene="secG"
                     /locus_tag="VHS97_14200"
     CDS             2416..2886
                     /gene="secG"
                     /locus_tag="VHS97_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX3449408.1"
                     /translation="MQFIDVILGILNVVIVLLTLFLIGIILIQRGKGGGLAGAFGGVG
                     GSSAFGTKTGDIFTKITVGVAIAWILLSMLMVVLTNRRNVSAFDDGSATSATKPLDSS
                     SSRSKDKDKSKPLEDVPAPSTTPSSAEGPPPIDIPVLPEAPVGTGKPNPSPAPK"
     gene            2899..3777
                     /locus_tag="VHS97_14205"
     CDS             2899..3777
                     /locus_tag="VHS97_14205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX3449409.1"
                     /translation="MLLSMTGFGEATRQEERWTIGVEVRSVNNRHFKLSSKISDAYAM
                     FEPALEQLVREQVRRGTVQVSLRIDRPKRPEDYRINMIALSSYRDQLKGLRGSDDRAF
                     DLGALLVLPGVVEDCRSSELVTDRAWPEISEVVQAALAKFQTARAAEGRAMAAELTAM
                     ARALDSHLGVIVARGPQVTQNHHRRLVERVSALVREHGVAVEPGDLVREVAILTDRSD
                     ISEEIVRLRAHLCQFCATIDEAESSGRKLEFISQEMGREINTIGSKAGDVEISRSVVE
                     IKALLEKIRELIQNVE"
     gene            3767..4375
                     /gene="gmk"
                     /locus_tag="VHS97_14210"
     CDS             3767..4375
                     /gene="gmk"
                     /locus_tag="VHS97_14210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX3449410.1"
                     /translation="MSSEGGPWATLPGRLVVISGPSGSGKSTLVRRLLARPDLRLKVS
                     VSVTTRSPRPGEVHERDYVFLTPEEFERKRGDLLESAQVHDHYYGTPAEPARLAMAEG
                     FCVILVIDVQGGFQVRQKVPDVLLVFVQPPSLEVLEARLRARGTDNEATIERRLTNAR
                     REIEMAAGYDVHVINDELEPAVERLAAILKQHGCGTESKGQR"
     gene            4372..4644
                     /locus_tag="VHS97_14215"
     CDS             4372..4644
                     /locus_tag="VHS97_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX3449411.1"
                     /translation="MIDELKEEEIVNKVGGRFKLSTLIQKRMIALNQGARALVEARGV
                     DKMTVVIQEIMQEKIYLDLSGKLRTVEPTEELEGNGETVDLTQPGD"
     gene            4802..>5833
                     /locus_tag="VHS97_14220"
     CDS             4802..>5833
                     /locus_tag="VHS97_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX3449412.1"
                     /translation="MSVRQRVLHAAPLIVFALTIVFLGLSLAGYDPADPPGRAAEPVN
                     QPPTNLCGPVGALFAHVLFTTLGWSSWLLILGLAVVNILAATRRPVPDKLGPALGFAL
                     VLIVAAGFFHKLAPTLMPSPAVGSGGYVGALAAIFLEVHFGLAGLILILTALGLFGLA
                     LCHDVIFLWPVQEVRAWIRPRPRLAHTAVHSHGAATMARAGAEEWERPSVLPAAAKAQ
                     SVSVHPPIPVTTANDAPPHRLQHPAITQDRVTSTARDLAPLPQVEPGSPFQLPPIEML
                     EPPTPAPIQEHEAKVHARAMLLERTLLDFGYQVRVVQIDTGPVITQFEIELEAGLRVS
                     RIMSLADDLA"
BASE COUNT         1131 a         1798 c         1783 g         1121 t
ORIGIN      
        1 cggctcgggc ctgaccatca gggccggccc agctactgac aatcgttgac ttccgggttg
       61 ataagcggct tgcccgggct cgcccatgag agaaagagta ggatgaagat taagatgagg
      121 aagaggatta cgagaaagag cacgattacg actaggattt actctgccgc gttgcttttg
      181 tcatgttcgc agctcgattt tctcttggtc agcctcatta gcatccaacc aactcgttaa
      241 agtgatcaaa ccagcattcg ttaggcacgg gcatgacgtc cttatcctaa tcttgatcct
      301 gctcttaatc ttcttcctag tcttaatctt catcctaatc ctactctatt tttggcatgc
      361 gaccggtcat atttacctgt caaccgattt tgctggcatc tccatcacag ccaatcctca
      421 caagtggtcc ttcacggaat gacaccgaga cgttacactt atccgttctt ccctcaatcg
      481 tcgttcaggt gggcacgaac gacatccgcc aagttggtcc tgcgaggagc cacgccggcg
      541 gccctaaaga aaggctctcc gaggatcgca atgatcattg tcttaaagcc tcactctacg
      601 cccgaggtca tcgagcatgt cctcgagcgc atcaacgcct tgggtttcac tccccatctc
      661 agccaggggg tggcgcggac gatcatcggg gtgatcggcg acgagaccaa gctccaggtc
      721 gagcctttgc aggcaattcc gggtgtcgag caagtcgtgc cgatcatgaa gccgttcaag
      781 ctcgccagtc gcgagtttca ccccgagccc tcggtgttcg agtccaaggg cgtcaagatc
      841 ggcggcggca acctggccgt gatcgccgga ccgtgtgcga tcgaaagcga atcgctcctc
      901 ttcgagatcg ccgcccgcgt gcgcgacgct ggcgccaata tcttgcgtgg aggggcgttc
      961 aagcccagga ccagccctta tagcttccag gggctgggcc ttgatgggct caagatcctc
     1021 caggcggccg gcgaccggtt tgaaatgccg gttgtgaccg aagtcatgga cccacgccag
     1081 gttgagctcg tcgaacgcta tactgatatt cttcaggtcg gggcgcgcaa tatgcagaat
     1141 ttcgacctgc tcaaggaatg cggacggaca cgcgtgccga tcttgctcaa gcggggcatg
     1201 agtgcgagcg tcaaagacct actcatgtcc gccgagtacg ttctgtcgga aggaaaccgg
     1261 caagtcatcc tatgtgagcg cgggatccga actttcgagg agtcgacccg gaatactctc
     1321 gacctatcca tcgtccctaa cgtccagggg caaagccact tgccgatcat cgttgacccc
     1381 agccacgcga ccggacgccc cgatctgatc ccggcgatgg cgcgggcggc gatcgccggg
     1441 ggggcggatg gtattcacat cgaagtccat tcctgccccg agcgagcgct ttcggatggg
     1501 ccccaggccc tcgtccccga ccagttcgac cggctcatgg acgaaatcca ccagctcgcc
     1561 gagctattgg gcaagtccgt cgactcttgc aaaggagagc cggtgacgtg cgcaccttga
     1621 tgatcgcggg caactggaag atgaatccgg cgacgcacgt cgaggcagtc gcactcgcgg
     1681 atgccgtcaa ggccggtgtg ggccaggaaa ccagcgtgcg tgtcgcgctt tgtccgcccg
     1741 ccgtttttct gtctcaggtc gacgcggcac tggccggcac gccaatcggt ctgggtgcgc
     1801 agaacatgca ttgggaaacc tccggcgcgc atacgggtga gatttctggg gcgatgctgg
     1861 tcgacattgg ctgcacgcat gtcattcttg ggcacagcga gcggcgccac ggcatgggcg
     1921 aaaccgatac ggttgtgaac gccaaactcc gggcggcctt gacgattggc ctaattccaa
     1981 tcgtctgttt gggcgaaacc aaagaccagc gcctggccga ccaaaccgaa gaagtgctcc
     2041 tggcgcagtt gaccggctca ttggcgggtg tcactcccga acagatggca gggacgatcc
     2101 tggcctacga gcccgtctgg gcgatcggaa cgggcctcac cgcgacaccc gaacaggccc
     2161 aagcggccca tcattttatc cgaagctggc tcaagggaca cttcggcgag gcgaccgcgg
     2221 cccgcgtcat tgtccagtac ggaggaagcg ttaaaccgga caacgccgcc gagcttttgg
     2281 catgcccgga catcgacggt gcgttggtgg gaggcgccag cctgaaagca ggcgacttcc
     2341 tcgccatatt ccgaggcggg ctcgacgtga ccccacgcaa gtcagcttga tcacaaaacg
     2401 caaagggttg aagccgtgca attcatcgac gtcatcctcg gcattctcaa cgtcgtcatc
     2461 gtcctcttga ccctcttcct gatcgggatc atcctgattc agcgagggaa gggaggaggg
     2521 ctggccgggg cgttcggcgg agtcggcgga tcgagcgcgt tcggcaccaa gacgggcgac
     2581 atcttcacca agatcaccgt gggtgtcgcg atcgcctgga tcttgctgtc gatgctgatg
     2641 gtggtcctta ccaaccgcag aaacgtgtct gccttcgatg acgggtccgc gacgtcagcg
     2701 acgaagccgc tcgattcgtc gtcgagcaga tcgaaagaca aggacaagtc aaagccgctc
     2761 gaggacgtac ccgctccgtc cacgaccccg agttccgccg aaggcccgcc gccgatcgat
     2821 atcccggtgc tacccgaggc gcctgtcggc acgggcaagc ccaatccgag tcccgcgccg
     2881 aagtgagcgg aggttgcctt gctcctgagt atgaccgggt ttggagaggc gacgcggcaa
     2941 gaagagcgct ggacgatcgg ggtcgaggtg cgctccgtca acaatcgcca cttcaagctc
     3001 agttccaaga tcagtgacgc ctacgccatg tttgagcccg cgctcgaaca acttgtgcgc
     3061 gaacaggtca gacgcggtac tgtccaggtg agtctgcgga tcgaccggcc gaaacggccc
     3121 gaagattatc ggatcaacat gatcgccctc tcaagctacc gcgaccagtt gaaagggcta
     3181 cggggttctg acgatcgcgc gttcgacttg ggagcgctct tggtcttgcc gggagtcgtc
     3241 gaagactgtc gctcgagtga actggtgacc gaccgggcct ggcccgagat ttccgaggtg
     3301 gtgcaggccg cgctcgcaaa gtttcagacc gcgcgggcgg cagaaggccg ggcgatggcg
     3361 gccgagctga ccgcgatggc acgggctctc gacagccatc tgggcgtgat cgtcgctcgg
     3421 ggcccgcagg tcacccagaa tcatcacagg aggctcgtcg agcgggtgtc ggctctggtc
     3481 agagagcacg gcgttgcggt cgagcccggc gaccttgtgc gcgaggtcgc gatcctgacc
     3541 gaccgctccg atatcagcga agagatcgtc aggcttcgag cccacttgtg ccagttctgc
     3601 gcaaccatcg acgaggccga aagctcggga cgaaaactcg agttcatcag ccaggaaatg
     3661 ggccgcgaaa tcaacaccat cggctcgaag gcgggcgatg tcgagatcag ccgctcggtc
     3721 gtcgagatca aggctcttct ggaaaagatt cgcgagctga tccaaaatgt cgagtgaagg
     3781 gggcccatgg gcaacgctac cggggcgatt ggtcgtgatc tccggtccat cgggatcggg
     3841 gaaaagcacg ctcgtgcgcc gattgctggc ccggcccgac ctacgtttga aggtttcggt
     3901 ctcggtcacg acgcgttccc cacggcccgg cgaagtgcac gaacgcgact acgtctttct
     3961 tacccccgag gaattcgagc ggaagcgggg cgatttgctc gaatcagccc aggtccacga
     4021 ccattattac ggtactcccg cagagccggc acgcctcgcg atggccgaag gattctgtgt
     4081 aattttggtg atcgacgttc agggaggctt tcaggttcgc cagaaggtac ccgatgttct
     4141 cttggtcttc gtccagcccc ccagcttgga agtcctggag gctcggctgc gtgcgcgcgg
     4201 taccgacaac gaggccacga tcgagcggcg gctcacgaac gcccggcgcg agatcgagat
     4261 ggctgcaggt tatgatgtcc atgtgatcaa cgacgagctc gagcccgcgg tcgaacggct
     4321 cgccgcgatc ctcaaacaac acggctgcgg aaccgagagt aaggggcaac gatgatcgat
     4381 gagctgaagg aagaagagat cgtcaacaag gtcgggggac ggttcaagct ctcaaccttg
     4441 attcaaaaac ggatgatcgc tttgaaccaa ggggctcgcg ctctggtcga agcacgtggc
     4501 gtcgacaaga tgacggtcgt gatccaggag atcatgcagg aaaagatcta cttagatctc
     4561 tccggtaagc tgcggaccgt cgagccgacc gaggagctcg agggcaatgg cgaaaccgtc
     4621 gatcttaccc aaccgggaga ttgaatagag cccgccgagg ggccgcaatt cttggagctg
     4681 gaatccgcaa ggccctggga ccgccggccg atcagatggt gagtagcgcg ctcttctcga
     4741 ccggataggc gtcgggctgt gtcgcgcggc accggtgacg tcggcgcttc cagggggtca
     4801 catgtccgta cgccagcgag ttctgcacgc ggccccgctc atcgttttcg ccctgacgat
     4861 cgtctttttg gggttaagcc tggcgggcta cgacccggcc gatccccccg gccgggcggc
     4921 cgagccggtc aatcagccgc cgaccaatct ttgcgggccc gtcggggcac tgtttgcgca
     4981 tgtgctattc accaccttgg gctggtcgtc gtggctactc atcctgggcc tcgcggtcgt
     5041 caatatcctt gcagctactc gccgccccgt ccccgacaaa ctcggcccgg ccctcggatt
     5101 tgcgctggtc ttgatcgtcg ccgccggatt cttccataag ctcgcaccaa cgctcatgcc
     5161 cagcccggca gtcggcagcg gaggctacgt gggcgccctg gccgccatct ttctcgaagt
     5221 ccatttcggg ctggccggct tgattctgat cctcaccgcc ctgggtcttt tcgggttggc
     5281 gctctgccac gacgtcattt tcttatggcc ggtccaggaa gtcagagcct ggatccgacc
     5341 gcgtccgcgg ctggcgcaca ccgcggtcca ctctcatggc gccgccacga tggcccgtgc
     5401 cggcgcggag gagtgggaaa gacccagcgt tctaccagcg gccgccaagg cccagtcggt
     5461 gtctgtccat ccgccgattc ccgtgacgac cgcgaatgac gctccgcctc accgacttca
     5521 acatccagcg atcactcagg accgtgtcac ttcgactgcg cgcgatctgg cccccctgcc
     5581 tcaggtcgaa cctggctccc cgttccagtt gccaccgatc gagatgctcg agccaccgac
     5641 tcctgccccg atccaggaac acgaagccaa ggtccacgcc cgggccatgc ttctcgagcg
     5701 gacgctgctc gattttggtt accaggtgcg cgtggtccag atcgatactg gcccggtgat
     5761 cacccagttt gagatcgagc tcgaagccgg cttgcgtgtt tcgcgcatca tgagcctggc
     5821 cgacgacctg gcg
//
LOCUS       DATAQL010000818         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3441580, whole genome shotgun sequence.
ACCESSION   DATAQL010000818 DATAQL010000000
VERSION     DATAQL010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3441580"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1440)
                     /locus_tag="VHS97_14225"
     CDS             complement(157..1440)
                     /locus_tag="VHS97_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449413.1"
                     /translation="MNLIITIALLVLLFSSRAWLEDGATQRGESISLEPKQFRAGACA
                     VDIAPATYPVLVNGGFLQNQATRLVDPVRAKSLVLDDGSTRLAIVVVDSCMMPRELLD
                     RAKELAREKTGILAERIMISATHTHSAPAAMGALGCRPDADYVARLPGWIAESIERAS
                     KLLEPARVGWNVIDDHEHTFCRRWIRRPDKMLDDPFGQRTVRANMHPGYENPDAIAPA
                     VPVDPGLTVLAVQARSGRPIAVLANYSMHYFGSVPVSADYFGRFATALAKRIGAQVVD
                     PPFIGIMSQGTSGDQMWMDYGQAKKNLGIDAYADAVAGYTQRAYEAINYHDWVSLAMA
                     ENRLVLGRRAPDASRLAWAREIVGRMTGRDTPNSLPEVYAKEAIVLHDEPRRELKLQA
                     IRIGELGIAAIPNEVFAITGLKIKAQCPLATTMNI"
     gene            complement(1465..2016)
                     /locus_tag="VHS97_14230"
     CDS             complement(1465..2016)
                     /locus_tag="VHS97_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449414.1"
                     /translation="MPLLYKLRHQGACHGSGVIAPDVRRPNRRFHGKLTAPNYAVQFL
                     AASARKPGGENGLTLREFVVLAKLVNAGDSPPPEELRGYDPDRGDLVGWRRVNDILEE
                     IPNMAGFQSPRLKIRFEPGDGPDNLKIIGPDGEPFLTYAELVKQRNADRQRAEEKARL
                     AEGQTQRADRLAARLRELGIEPD"
     gene            2038..>2454
                     /locus_tag="VHS97_14235"
     CDS             2038..>2454
                     /locus_tag="VHS97_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3449415.1"
                     /translation="MLTALLVSVLVSASYLDSTSIPAFGVAADPDTELPRGGFFGAKL
                     ISVSEDVRERFKLKAGDGVAIEAVIPGSTAAAAGFQAGDVLLAVNGATIAGTGGFVQE
                     IASRKAGAELTIELRRGEDLRQEKVTLKGRPFEKSDE"
BASE COUNT          445 a          720 c          779 g          510 t
ORIGIN      
        1 tttgtcggcg actttctcga gcaatccgag tacggtctcg acgattctcg gctcggcctg
       61 aacttcgagc cctgcggtcc gtgccggcca ggtcgtatag cctcccagcg cgtgctgttc
      121 ggggggcgga atgtagccct cggaaccatt tgccagctag atattcatcg tcgtcgcgag
      181 cgggcattga gccttgatct tcagcccagt gatggcgaac acttcgttgg ggatcgccgc
      241 gatgcctagc tcaccgattc ggatggcctg gagcttgagc tcacgccgcg gctcgtcatg
      301 cagaacgatc gcctctttgg cataaacctc cggcaggctg ttgggtgtgt ctcgacctgt
      361 catgcggccg acaatctcgc gcgcccacgc cagccgggaa gcgtcgggcg cgcggcgacc
      421 gagcacgaga cggttttcgg ccatcgccag cgaaacccag tcgtgatagt tgatcgcttc
      481 gtatgcccgt tgagtgtaac cggcgaccgc gtcggcgtag gcatcgatgc caaggttttt
      541 cttggcctgg ccatagtcca tccacatctg gtcgccgctg gttccttgcg acatgattcc
      601 gataaacggc ggatcgacca cttgggcgcc gatccgtttg gccagtgctg tggcgaaacg
      661 accgaagtaa tcggctgaga cgggaaccga tccaaagtag tgcatggagt aattggccag
      721 cacggcgatg ggccgcccgc tacgcgcctg gaccgcgagc accgttaacc ccggatcgac
      781 tggcaccgcg ggcgcgatcg cgtcggggtt ttcgtagccg ggatgcatgt tggcgcggac
      841 cgtccgctgg ccgaacgggt cgtcgagcat cttatcggga cggcggatcc agcgccggca
      901 aaacgtatgc tcgtgatcgt cgatcacgtt ccagccaacc cgcgccggct cgaggagctt
      961 gctagcacgc tcaatcgact cggcgatcca tcccggcagg cgcgccacgt aatcggcatc
     1021 gggccggcag cccagtgctc ccatggcggc aggcgcggag tgggtgtgcg tcgccgagat
     1081 catgatccgt tccgccagga tgccggtctt ctcccgggcc agttccttgg cccggtcgag
     1141 caactcgcgc ggcatcatac aactatcgac cacgacgatc gccagccgcg tcgaaccgtc
     1201 gtcgaggacc aagctcttgg ctcggaccgg gtcgacgagc cgggtagctt ggttttgcaa
     1261 gaagccgccg ttcaccaaga ccggatacgt agctggcgcg atatcgacgg cgcaagcgcc
     1321 tgcgcggaat tgctttggtt cgagcgatat tgattctccg cgctgagtcg ctccgtcttc
     1381 taaccatgct cgactcgaga acaggagcac gagcagagcg attgttatga tgagattcaa
     1441 ggttgtctcg gtgtgtcaac ggtctcaatc tggttcaatg cccaactctc gcaatcgcgc
     1501 ggccaggcgg tcggcacgtt gggtttgtcc ctcggcgagt cgggctttct cctcggcgcg
     1561 ttggcgatcg gcgttgcgtt gcttgaccaa ctctgcgtaa gtcagaaacg gctcgccatc
     1621 gggcccaatg atcttgaggt tgtctggtcc gtcgccgggc tcgaatcgga tcttaaggcg
     1681 agggctctgg aagccggcca tattgggtat ttcttccagg atatcgttca cacgtcgcca
     1741 gccgacaaga tcaccgcgat cggggtcgta gcctcgaagt tcctcgggtg gcggtgaatc
     1801 gcccgcatta acgagtttcg caaggacgac aaactctctc aaggtcagcc cgttttcgcc
     1861 tccgggctta cgtgctgacg cagccaggaa ctggacagcg tagttaggcg ccgttagctt
     1921 accatggaag cgccggttcg gtcgccttac atccggcgcg atcacccctg agccgtgaca
     1981 tgcgccttgg tgtctcagtt tgtacagtaa aggcatgtga actgggagga gccttccatg
     2041 ttgacggcgc tactggtctc ggtacttgta tccgcctcct acctggattc gacgagtatt
     2101 ccagcgttcg gtgtcgcggc tgatccggac acggaattac cgcgaggcgg gttcttcggg
     2161 gcgaagttga tatcagtttc cgaagacgtc cgcgagcgtt tcaagctcaa agctggcgat
     2221 ggagtcgcga tcgaagcggt aattcccggc tcaaccgcgg cggcggcagg ctttcaggcg
     2281 ggcgatgttc tgctcgcggt caatggtgcg acaattgctg gaacgggcgg attcgtccag
     2341 gagatcgcca gccgaaaagc gggtgccgag ttaacgatcg agctgcgtcg gggagaggat
     2401 ctgcgacaag aaaaagtgac gttaaaggga cgtccgttcg agaagagcga cgag
//
LOCUS       DATAQL010000819         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4816293, whole genome shotgun sequence.
ACCESSION   DATAQL010000819 DATAQL010000000
VERSION     DATAQL010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4816293"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VHS97_14240"
     CDS             complement(<1..517)
                     /locus_tag="VHS97_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449416.1"
                     /translation="MRLRLFARCGLALVWALGLLFLSACQTQEPDPTATPAPAGNEIA
                     SRQPPPEPLKNERPAAALADVMSAHFQGLGHMERFEYREAAEAFRHVRNRAPGWIPGS
                     INLAIALLNDSGVQAEQAKKAGGRETAPNNFDEALALLAGVLEREPASPHAHFCRGII
                     LEQQGRLVDAHG"
     gene            complement(647..1585)
                     /locus_tag="VHS97_14245"
     CDS             complement(647..1585)
                     /locus_tag="VHS97_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX3449417.1"
                     /translation="MPVIDRYYAAACQTDLPCPRDRGEITMRVGRLLAMVDRAVVGYE
                     PFFDIKLVVFPEFAHAVPIYETAQELSDRLAVPIPNEHTDRYIQKARERSVYIQTGTF
                     LEVDPRWPQAVFNTTCLIGPDGILSRYRKVNPWLPWEVHASPHDLAGYDEPLFPVVET
                     EIGRLGAAICYDWLFPEAIRALALGGAEVLIRVSAYMDPWGATPPLDWWTLFNRARAV
                     ENFAFVVAANQGASAENYPPFSWPGGSMIVDFDGRILSQADPGPGEKIVVGPIDLAAL
                     RAERLRRRGHHFLSHLRAEVYTELYTRPIFRTVERD"
     gene            complement(1667..>2372)
                     /locus_tag="VHS97_14250"
     CDS             complement(1667..>2372)
                     /locus_tag="VHS97_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449418.1"
                     /translation="VVAGGGYWFGGSPFLVMGPGGFLPPFALMGPSFLPARAPLMAPP
                     PPGFLGRNGAPAANQAQAKPADPARSAQLTTLGDRLFRAGNLKKAEERYLQAMRAAPD
                     LAAPRVRLAQVALARGHFADAATRLREAETAQPGWILNAPDIQTIYGEPTEFARSLAR
                     VESYLQAHPDDRDGWLVLGAQLFLSGRTTKAADVFKRLDDPNRKSDVALAAFLDASNQ
                     AVRQPGNGPVRIDSLR"
BASE COUNT          498 a          705 c          746 g          423 t
ORIGIN      
        1 ggccatgagc gtcgaccaga cgtccctgct gctcgaggat gataccacga cagaagtgag
       61 catgagggct ggctggctct cgttccaaga ctccggcgag aagagctagg gcttcgtcga
      121 aattgttggg ggcagtctcc ctccctcctg ctttcttggc ctgctcggcc tggaccccac
      181 tatcattgag gagagcgatg gccaggttga tcgagccagg aatccagccc ggcgcccgat
      241 tgcggacatg gcgaaaggct tcagccgcct cgcgatactc aaatcgctcc atgtggccca
      301 gtccctggaa gtgcgccgac atgacgtccg cgagagcggc tgcaggtctt tcattcttga
      361 ggggttctgg tggcggctgc cgggaagcaa tctcgttccc ggcgggagcc ggagtcgcgg
      421 tgggatcggg ttcttgtgtc tggcatgccg acaggaataa gagcccaagt gcccagacca
      481 aagcaagtcc gcagcgcgca aagagtcgca atcgcatcgt cctgctcccc cgggggaatg
      541 atcagtggtg gataaaagat gatctcggca cccgaccgct acaaagtatt caacatagga
      601 tgatcggact gtcgacgaac gcgttcgtac gggaacagca tggtcatcaa tcgcgttcga
      661 ccgttcgaaa gatgggtcga gtgtaaagtt cagtatagac ttccgcccgc agatgggaaa
      721 ggaaatggtg accgcggcgc cttagccgct cggctcgcag cgccgccaga tcgatcgggc
      781 cgacgacgat cttttcaccg ggtccgggat cggcctgaga cagtatgcga ccgtcgaaat
      841 caacgatcat gctaccgccc ggccatgaaa atggaggata attctcggcg ctcgcaccct
      901 gattcgcggc aacgacgaag gcaaaatttt cgacggcccg agcgcggttg aagagggtcc
      961 accaatcgag tgggggcgtc gccccccaag ggtccatata tgcagacacg cggattaaga
     1021 cctctgcacc tcccagtgcc agtgcacgaa tggcttcggg gaaaagccag tcgtaacaaa
     1081 tggcggcccc cagacgtccg atctcggtct cgacgaccgg aaacaagggc tcgtcatagc
     1141 cggccagatc atgcgggctg gcgtgcacct cccagggaag ccatggattc accttgcggt
     1201 agcggctcag gatcccgtcg ggcccgatca agcaggttgt gttgaacacc gcttgaggcc
     1261 acctgggatc gacctccaga aacgtccccg tctggatata gaccgatcgc tctcgtgcct
     1321 tctgaatgta gcggtcggtg tgttcgtttg ggatcgggac agcaagtcgg tcgctgagct
     1381 cttgggcggt ctcgtaaatg ggaacggcgt gggcaaactc ggggaaaacc accagcttga
     1441 tgtcaaagaa cggctcgtac ccgacgactg ctcggtccac catcgccagt agccgcccca
     1501 cgcgcatggt gatttcaccg cggtcgcgcg gacaaggcag atcggtctga caagcggcgg
     1561 cgtaatagcg gtcgataaca ggcatgtcac tttaacaagc gacggcctct agacgcgtcg
     1621 cgagtccaaa agaacggccg ccctgatgtt tcaaccgccg ggacttctac cgaagagagt
     1681 caattcggac ggggccgttg cccggctgcc gcacagcctg gttggacgca tcgaggaagg
     1741 ccgccagtgc gacgtcggac ttgcggttcg gatcatcgag tcttttgaag acgtcggccg
     1801 ccttggttgt tcgacccgag aggaacaatt gggctcccag gacgagccac ccatcgcggt
     1861 cgtcgggatg ggcctgcaga taggattcga ctcgcgccaa ggaccgcgcg aactccgtag
     1921 gctcgccata aatcgtctga atatcggggg catttaagat ccagccgggt tgggcggttt
     1981 cggcctcacg aagccgagtg gcggcgtcgg cgaaatgccc cctggcgagc gcgacctgcg
     2041 ccaaccggac acgcggtgcg gccagatcgg gcgctgcgcg catcgcctgc agataccgtt
     2101 cttccgcttt cttgagattg ccagcccgaa acaatcgatc acccagcgtg gtgagttgcg
     2161 cggagcgagc agggtcggcg ggtttagcct gcgcctgatt ggcggccggc gcgccattgc
     2221 gaccgagaaa gcccggcggc ggtggcgcca tgaggggcgc tcgcgcaggc aggaacgatg
     2281 gtcccatcaa tgcaaacgga ggtagaaaac cccctggtcc catcaccaag aatggagaac
     2341 cgccgaacca atatccaccc ccagccacaa cc
//
LOCUS       DATAQL010000820         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4511329, whole genome shotgun sequence.
ACCESSION   DATAQL010000820 DATAQL010000000
VERSION     DATAQL010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4511329"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VHS97_14255"
     CDS             <1..299
                     /locus_tag="VHS97_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449419.1"
                     /translation="TPPGSGCSVTFGKGLTAAAPGSAAGGLIVSDIEAAHDELIRRGI
                     DASEVWHGPPFPTEARVSGPDPQRTSYGSFFSFNDPDGNTWLVQEVTTRLSGRI"
     gene            complement(511..1065)
                     /locus_tag="VHS97_14260"
     CDS             complement(511..1065)
                     /locus_tag="VHS97_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX3449420.1"
                     /translation="MGKLVFAMSQSLDGYVDGVDGGLQLPLHQPPDAALFRHFTDQVR
                     ARAGSLYGRRIYEMMRYWDEDRPEWGALERDFAAAWRARPKWVVSRSSKSVGANATLV
                     GNDVEAFVRGLKAKVEGDIEVAGPDLAGSLTDLGLIDEYRLYFRPFVLGRGKPYFARA
                     RPPLRLVATEPVGEDAVRLIYIPA"
     gene            complement(1201..1767)
                     /locus_tag="VHS97_14265"
     CDS             complement(1201..1767)
                     /locus_tag="VHS97_14265"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX3449421.1"
                     /translation="MKNLLTSYARLTTLRWQSWYAIPLRLIVGYGFMEHGYAKLVHGP
                     DSFTAILHALGMPAPALLAWATILVELLGGFAVLVGAFIPIASIPMVITLLVAIFTVH
                     VPNGFSSIKLLSVDAAGAHFGQPGYETDLLYMAGIFAIVLGGSGRLALDRLLLRRFGV
                     TEHSALGFRNVRQDRPTGVQPAGSEKQA"
     gene            complement(1961..>2150)
                     /locus_tag="VHS97_14270"
     CDS             complement(1961..>2150)
                     /locus_tag="VHS97_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449422.1"
                     /translation="LNGGPDGRAADHKAFNQNVLRRQAGPRRQGAAGDLKHKLLFELS
                     MKGKRGSDIKPHVDIVTS"
BASE COUNT          444 a          676 c          634 g          396 t
ORIGIN      
        1 tcacgccccc cggctcaggg tgctcggtca ccttcggcaa gggactaact gcggcggcgc
       61 ctggctcggc tgcgggaggg ttgatcgtct ccgacatcga agcggcccat gacgaactca
      121 tccgccgtgg catcgacgcg agtgaggtgt ggcacggccc gccattcccc acggaggcac
      181 gggtgagcgg tcccgatccc cagcgcacga gctacgggtc gttcttttcc ttcaacgacc
      241 ccgacggcaa tacgtggcta gtccaggagg tcacgacgcg gctctccggc cgaatctgat
      301 acggcattcg cattcacgga gcaacagacc gcggcgaaag cttctactgc gatatctggt
      361 cgggtcccgc gcctgacggt tggcggatcg ataaagaggc gacaatgttc aagatggttt
      421 gggaaggtgt tagctgaatt cccgcatgcg tcgcagacct atgtccgctg tctcaggtcg
      481 gctcaggcgc gaaaaagggg agcaacacaa tcaggcaggg atatatatca acctgaccgc
      541 gtcctcgcca acgggttcgg ttgcgaccag gcgaagtggt ggccgagccc tggcgaagta
      601 cggcttgccg cggccaagca caaaagggcg aaaatagaga cgatactcgt cgataagacc
      661 gaggtcggtg agactgcccg ccagatcggg cccggcaact tcgatgtcgc cctcgacttt
      721 cgcctttagc ccgcgcacga atgcttcgac gtcgtttccg acaagcgtgg cgttcgcgcc
      781 taccgatttc gaggaacgtg acacgaccca tttcggccgt gcccgccacg ccgctgcgaa
      841 gtcgcgctcg agcgcgcccc actccggccg atcctcatcc cagtagcgca tcatctcgta
      901 gatgcgacga ccgtacagac tgccggctcg ggcgcgaacc tgatccgtga agtgacgaaa
      961 gagcgcggcg tcgggcggct ggtggagcgg caattgcagc ccgccatcca cgccgtcgac
     1021 gtagccgtcg agggactgcg acattgcgaa gaccagcttc cccataacct cacacctccg
     1081 cacagttcct ggtcaactcg gctgagtcac ctcggttggt gtgcctacct taggctttat
     1141 tagtctacag tacgccgcgt ggtcacccgc cgccaatcac aatgaggttc ttcagcatgg
     1201 ctatgcctgc ttctcacttc ctgccggttg gacgccggtt ggacggtctt ggcgaacatt
     1261 ccgaaagcca agggcagagt gctccgtcac gccgaagcgc cgcaacaaga gacgatccag
     1321 cgcaagccgc ccggagccgc cgagcacaat ggcgaagatc cccgccatgt agagaaggtc
     1381 agtctcataa cccggctgcc cgaagtgcgc gccggccgcg tctacagaca gcagcttgat
     1441 agaactgaac ccgttgggca cgtggacggt gaaaatcgcc acgagcagcg tgatcaccat
     1501 gggaatgctc gcgatcggaa taaaagcgcc gacgagcacc gcaaagccgc cgagcaactc
     1561 gaccaagatc gtcgcccagg caagcagagc tggggccggc atccctaagg cgtgcaagat
     1621 tgccgtaaac gaatccggac cgtgcacgag ctttgcgtag ccgtgttcca tgaagccata
     1681 gcccacgatc aggcgaaggg gtatcgcata ccaggattgc caccggagcg tcgtgaggcg
     1741 tgcataggac gtaagcaagt tcttcattcg ggcaccttct tttcgagatt aacgattgct
     1801 ctacgagcct gcccaccgat ttgttacaag tcggctgatg acaatttcat aacgtgacca
     1861 gcccgcgtcc atgcgcccgg tgcttcatgc gcccgttgct tcaaacgtcc cccgtgcgtc
     1921 gggcgccgcg gaagatcctc ttttgattga catagggaca tcatgatgtc actatgtcga
     1981 catgcggctt gatatcagat ccccgcttcc ccttcatgct cagctcgaag agcaacttat
     2041 gcttgagatc gccagcggcg ccctggcgcc tgggaccagc ctgccgccgg aggacgttct
     2101 ggttgaacgc tttgtggtca gccgcacgac cgtcaggacc gccattcaaa
//
LOCUS       DATAQL010000821         4729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_543184, whole genome shotgun sequence.
ACCESSION   DATAQL010000821 DATAQL010000000
VERSION     DATAQL010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543184"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..359)
                     /locus_tag="VHS97_14275"
     CDS             complement(102..359)
                     /locus_tag="VHS97_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449423.1"
                     /translation="MTDIAMTIEQTRAAIQARPFKPFVMHLADGGSIPVEHPEFFLTV
                     PSGRTVIVVQPDDTTNIVDLLLVSHLEILPSSGTPRGHKRS"
     gene            complement(407..1666)
                     /gene="rho"
                     /locus_tag="VHS97_14280"
     CDS             complement(407..1666)
                     /gene="rho"
                     /locus_tag="VHS97_14280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX3449424.1"
                     /translation="MHLTELQRMTMPQLIRTAKTEGIVDYMGLKKQDLIFKILKERVK
                     QNGLMYGEGTLEVLPDGFGFLRSPDYNYLPCPDDIYVSPSQIRRFGLKTGAIVSGQIR
                     PPKENERYFALLRVEAINFEDPDKLSEKVGFDDLTPLHPQGRIRLETTPEEINMRVVD
                     LVTPLGFGQRGVIVAPPRTGKTILLQKIANSILTNHPESYVMVLLIDERPEEVTDMER
                     SVKGPTAEVISSTFDEPASRHIQVAEMVIEKAKRMVEFGKDVVILLDSITRLARAYNS
                     EAPHSGKILTGGIDASALQKPKRFFGAARKIEEAGSLTILATALVDTGSRMDDVIFEE
                     FKGTGNMELHLERRLVDKRIWPAIDVNRSGTRREELLMDAEELRRVWILRRVLNDMNP
                     VEAMELLTGRMRKTKLNAEFLMSMNLT"
     gene            complement(2538..3254)
                     /gene="coaE"
                     /locus_tag="VHS97_14285"
     CDS             complement(2538..3254)
                     /gene="coaE"
                     /locus_tag="VHS97_14285"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX3449425.1"
                     /translation="MTQSPSTREPRAAERKPHGDLVVIGLIGGIGGGKSSVAALLEDA
                     GAVVIDADVVGHELLNEPSVRSQIVERFGMGVLSARSADPGLPSAIDRKALGAIVFAD
                     PLARRDLETILHPRMRARFEAVIEHGAEFRSAEKSMIVLNAAILLEAGWDDLCDLIVF
                     VDAPRDERMRRVQEERGWSREVFEAREEAQWPCDLKRQRADLVITNDAGVDGLKREVQ
                     RVQARLAELSCPLIETSNSR"
     gene            complement(3777..>4729)
                     /locus_tag="VHS97_14290"
     CDS             complement(3777..>4729)
                     /locus_tag="VHS97_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX3449426.1"
                     /translation="ALKHPFPKLLLQHRQLSKLKSTYLDALPGLVHPEDNRIHASFNQ
                     SVAATGRLSSSDPNLQNIPVRTEDGRQIRQAFIAGRPGWLLLTADYSQIELRVLAHYS
                     ADPALFRAFANDLDIHKAVAARIFGVAETAVDDSMRRVAKTVNFGVVYGLSAYGLASR
                     LGISQTEAATFIESYFQEYAGVDRFITQTLEFAQAKGYVATLLGRRRPINGIKSTTGR
                     TRNLAERTAINTVIQGSAADLIKRAMTMLDQELRTHKFEARMLLQIHDELVFEAPAAE
                     LPRLADLVNRVMTSALHLSVPLKVDIAAGHNWLDVDSIPQ"
BASE COUNT          897 a         1411 c         1445 g          976 t
ORIGIN      
        1 aagagcgatt cggtaacaag cccattgaag cgcgaggcga cgatcgcaaa ccggccgacc
       61 ggaggagaat aatcaccgat gtactcggcc atagcctttc atcacgacct tttgtgcccg
      121 cgcggcgtcc cactactggg caggatctcg agatgcgata ccaagagtaa atctacgatg
      181 ttcgtcgtat cgtccggctg cacgacgatg acggtccgac cactcgggac ggtcaggaag
      241 aattctggat gctcgacagg aatgctgccg ccatcggcaa ggtgcatgac aaatggcttg
      301 aacggacgcg cctgaatcgc agcccgcgtc tgctcgatgg tcattgctat gtcagtcacc
      361 tcagcctttt cctgatcgtc cctccgcgtt catccttcca cctttctcac gtcaggttca
      421 tgctcatcaa gaactcggca ttgagcttgg tcttacgcat tcttccggtc aagagctcca
      481 tggcctcgac ggggttcatg tcattcaata cgcgacggag aatccacacc cgacgcagct
      541 cttcggcgtc catcagtagc tcttcccggc gcgtgcccga acggttgacg tcgatggccg
      601 gccagatccg cttgtcgacc agccggcgtt ccaagtgaag ctccatgtta ccggtcccct
      661 tgaattcctc gaagatgacg tcatccattc ggctgccggt gtcaaccaag gcggtcgcca
      721 ggatagtcag gctgccggct tcttcgatct tgcgggccgc gccgaaaaac cgcttgggct
      781 tttgcagggc cgaggcatcg atgccgccgg tcaagatctt gcccgaatgg ggtgcctcgg
      841 agttgtaggc ccgcgccagc ctggtgatcg aatcgagcag gatcacgacg tccttaccga
      901 attcgaccat ccgcttggcc ttttcgatga ccatctcagc cacctggatg tgcctcgagg
      961 cgggctcgtc aaatgtcgag ctgatgacct cagcggttgg ccccttgacc gagcgctcca
     1021 tatcggtcac ttcctcgggg cgctcgtcga tcaacagcac catcacgtat gactcgggat
     1081 ggttggtgag aatgctgttg gcaattttct ggaggagaat ggtcttacca gttcggggag
     1141 gagcgacgat cacgccgcgc tggccgaagc caaggggggt gaccaggtcg acgacccgca
     1201 tattgatctc ttcgggagtg gtctccagcc ggatccggcc ctggggatgc agcggtgtca
     1261 ggtcgtcgaa accgaccttc tcgctgagct tgtctggatc ctcgaagttg atcgcttcga
     1321 cccgtaaaag agcgaagtag cgttcgtttt ccttgggggg ccggatctgg cccgagacga
     1381 tcgcacctgt cttcaagccg aagcggcgga tctggctggg tgaaacatag atatcgtcgg
     1441 ggcaggggag gtagttgtaa tcggggctcc gcaggaagcc aaagccgtcg ggaagaactt
     1501 cgagcgtccc ctcgccgtac attagaccgt tttgcttgac ccgttctttc aaaatcttga
     1561 agatcaggtc ctgcttcttg agccccatgt agtcgacgat cccttcggtt ttggccgtcc
     1621 ggatcaactg gggcatcgtc atccgttgga gctcggtgag gtggatctca ccgcgcttta
     1681 tgtcttcgta acgatcgtgg agcgcggcgt cgccgaagat gtcttcttcg tcttcgggaa
     1741 agccggcggg cgcgtcggca acggtggccg gtacagggcg ttcaacgcga gcgggcggcg
     1801 gcccgtagtc tcgctcgcgc gaggcatcgc gctcgcgagg gccgtaggtt ggttcgcgct
     1861 cggggcgttc ccggaaattg gccgtgttac caccgtcgaa ttcacgcgtc cgctctcgcg
     1921 cgggcggcgt gggctcccgg tcacgttccc gttcgcgagc gatcggtatt ccttcgtggt
     1981 ggatcggtcc gccgtcgcgc tcgcctcggt cgcgcgccag ccgctcccgc accgagagtc
     2041 ctggttcacg ctcgaccggc tcgcggaccg gctcgcggtc gcgctggcgg atgaaacgga
     2101 tcggctcgcg atccgccggg ccttcttcac gaaagcgctc gcggtcctgg cgatcgcgct
     2161 ggttttccgc cggtcccgga gacgactcgc tcgccgggga atatgacggg gccgggccag
     2221 gtgctgtgcc gctcgaatcg tccgcggccg gcggcgtcga cggcgctgcg gtcttcctga
     2281 tgcgggatgt gccggttggc tctcggcgtg ggcgagtatc gttggccatg gggatccatt
     2341 ctcctgtaca aatcaaacgg aaacggtgaa tcgttcgagg cccaaagaca cgaccgggct
     2401 cgattcgcag ctaaccaggc aacctaaaaa cgcttgaatc gttacggacc gagccaaccg
     2461 ctcaggagcc cgtgcacgcg gccgcctggc gcaggccgtg gttgagatgt ggcaaagctt
     2521 caagcgatcg tgagcgttca tcttgagttc gatgtctcga tcaatggaca tgacagttcc
     2581 gcgagccgcg cttgcacgcg ttgcacttct cgtttcaagc catcgactcc agcgtcgttg
     2641 gtaatgacga gatcggcacg ttgccgcttt aggtcgcacg gccattgtgc ttcctcgcgg
     2701 gcctcgaata cttcccgcga ccagccgcgc tcttcttgaa cacgacgcat ccgctcgtca
     2761 cgaggagcgt cgacaaatac gatcaaatca cacagatcat cccaaccggc ctcgagcagg
     2821 atcgcggcat tgagcacgat catacttttt tctgcacttc tgaactcagc cccatgctcg
     2881 atcaccgcct cgaaccgggc ccgcattcgc ggatgcagta tcgtttcgag gtcgcggcga
     2941 gccaggggat ctgcgaagac gattgccccc aacgccttgc gatcgatcgc cgacggcaat
     3001 cccgggtcag cacttcttgc cgatagtaca cccatcccaa atcgttcaac gatctgactg
     3061 cggaccgatg gttcgttcaa aagctcgtgc cccaccacgt cggcatcgat cacaaccgcc
     3121 cccgcgtcct ccaagagcgc ggcaacgctg ctcttgcctc cgccaatgcc accaatgagc
     3181 ccgatcacga ccaagtcacc atgcggctta cgctccgccg cgcgcggttc tcgggttgat
     3241 ggggattgag tcatcgacga gaagctccgt agcatccgaa tggcaggctg cgcgttcgca
     3301 tgagccgcag gatcaagatc gatcgtctcc gagccaacgg caccagcccg agacccatcg
     3361 ggacagttgc gcggtcggct gagagcaaat gaaccgccca gtcaatcacc gaaggaggag
     3421 ccggtgcgta gtcgtgggct gaaaattgga acaggcggca cgaaacgtga ttcgcgcaac
     3481 gtggtaaatt gattaaacca ggtgggtttc ctgaaggtgt cccagccgtc gtcactcatt
     3541 ccagggcttc tcgaagcatc agaagggccg tggcagagga ggacaggcgt gatgaagggt
     3601 aaaaaaatct gcggcggatc tgtgttgatc gttgctcgat catacgcaat catgcctcac
     3661 gctgcaatgg gtctccccgg ttccggtcaa aaaacgggtc tcggtcacac ggtcgccaac
     3721 ttccgccttg aagggattcg gtcgtcgcag cttgattatt ttagattgag attgcgtcac
     3781 tgcgggatcg agtcaacgtc gagccagttg tggccggcgg cgatatcgac cttcaacggg
     3841 accgacagat gcagggccga tgtcattacg cggttcacca ggtcggccaa ccggggaagc
     3901 tcggccgccg gcgcttcaaa gaccagttcg tcgtggatct gaaggagcat ccgggcctcg
     3961 aacttgtgcg tgcgtagctc ttgatcgagc atggtcatcg cgcgcttgat gaggtcggcc
     4021 gcggatccct ggatcacggt atttattgcc gtgcgttcgg cgaggttgcg tgtacgtccg
     4081 gtcgtcgact tgatcccatt gatcggccgc cgccgtccaa gcagtgtcgc aacataaccc
     4141 ttcgcttgcg cgaactcgag cgtttgggtg atgaaccggt ccacaccggc atattcctga
     4201 aaataagact cgatgaatgt ggccgcctcg gtctgggaaa tgccgaggcg gcttgccagc
     4261 ccgtaggccg atagcccgta gacgacgcca aaattgacgg tcttggcgac gcggcgcatt
     4321 gaatcgtcaa cagccgtctc ggcaacgccg aagatccgcg ctgccacagc cttgtgaatg
     4381 tcgagatcgt tggcaaacgc ccggaagagt gccggatcgg cggaataatg ggcgagcacg
     4441 cgcagctcga tctgcgagta gtctgctgtc aaaaggagcc agcccggccg gcctgcaatg
     4501 aaagcctggc ggatctggcg accgtcctcg gtgcgcacgg ggatattctg caagttgggg
     4561 tcactcgagc tcagccgccc cgtcgcggcg acactctggt tgaacgacgc gtgaatgcgg
     4621 ttatcctcgg gatgcacaag tccggggagt gcgtccaaat aggtgctctt gagtttggag
     4681 agctggcgat gctgcaagag tagcttgggg aatgggtgct tgagcgcca
//
LOCUS       DATAQL010000822         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1764972, whole genome shotgun sequence.
ACCESSION   DATAQL010000822 DATAQL010000000
VERSION     DATAQL010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764972"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..345
                     /locus_tag="VHS97_14295"
                     /pseudo
     CDS             1..345
                     /locus_tag="VHS97_14295"
                     /inference="COORDINATES: protein motif:HMM:NF023950.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
     gene            1047..1646
                     /locus_tag="VHS97_14300"
     CDS             1047..1646
                     /locus_tag="VHS97_14300"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX3449427.1"
                     /translation="MSDRSSKLATFIDGTNLHATTKALGFDIDYKRLLQEFRSCGTLV
                     RAFYFTMLTEDQEFVSIRPLIDWLDYNGYTVITKMSKQFVDPAGHRKLKGDIGVELAV
                     QAMELAQYIDQLILFSGDGAFRSLVAALQRRGVRVSVVSTMACQPPMVADELRRQADV
                     FIDIKELQVSVERDPNHRQTQRDYRHLSPNFVGRQSRSH"
     gene            1837..3072
                     /locus_tag="VHS97_14305"
     CDS             1837..3072
                     /locus_tag="VHS97_14305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3449428.1"
                     /translation="MRVCITMVSLVAFSGPLFFLEPAIAQQSKGPAVGIVEVVNRPII
                     ERTEFLGRIQAVDRVNVVARVTAFLEKRLFKEGAEVKANDRLYLLERGPFEADLEAKK
                     AQVAQLEATLENAQLTADRQRTLLSGPAGLPANYDTAVSNQKSLEAQVEAAKAQVSFS
                     QINLNYTDIRSPIDGKVDRTSVTEGNVVSPTSGVLTTIVSQDPMYVVFPVSVREALSL
                     RDRYAAKGGRKAVVISLKLPDGRIYGQTGRIDFASNSVTESTDTLLVRGEIANPLLSP
                     SASNATRELTDGELVTVMLEGAQPVQTLAIPRAAVLSDQQGNYVLVVGAENKVEQHRV
                     KLGQSSQTIASVMNGLSLGDKVIVEGLQRVKPGQVVEPGPASGEVQAMMKSTGGAAQG
                     TGSKGESVLGTSSTQPNGG"
BASE COUNT          710 a          958 c          948 g          636 t
ORIGIN      
        1 cgcgccagca ctgatcaatt caaatatgcg cagcgtgtcc gcaatgcggt tgcgtgcttg
       61 ctcgacaatc ttccggacga catgaagaac cagccggcgc cgtcgaactg cttcgaccgg
      121 tcgcggacag gaaggcgtac aacctcgttc atctgattta ccgcgccagc acgtacgaag
      181 gataatcgaa aagactacga attctcgcga cggagcaggg aaatgcactg gcggacgggc
      241 taccacgata cggtacggac gctccgccat cccaagatcc tcgagcgact gcttggtgta
      301 gacggggtgg caatcttcga tctcgattac gacgggcgcg attagctcgt gctgaccgtg
      361 accaggagcg cgttaaagac aatcggacct caagccgaat gctccgttaa tcctggagtc
      421 gccctttctg acgcgcgggt cgcttgcagg cagaaatccc gccaacggaa gcaggagcag
      481 gccggcatcg agacgcttag tctaagtcta gagtcgtacg cctgcacaaa actgttccga
      541 tcggaatcga acccgcgttc gcagaccccc tgcggatcac ggcggctact agccccagat
      601 gccgaccgct atgagcgcga cgacagcgat cccgattacc caatatggcc aaaccgcttt
      661 gcactcgtcg cacgcgcttc cgttccaaga ttcattgatc tcgtcccgac gctggccgaa
      721 gaggggcatc atacgacagt cgtccatcgg gccaggaaat cgcccggcgg cgcctcttcg
      781 ccgaggtcta cgaactcggt gatcgtctta actgcttgcc gagacacaat agcacaacgc
      841 aagggcttac attggtgaat ttcgctcagt gacacacgca tcaaattgag cttctcatcg
      901 gggcgatccc gacggtatat ccgcaggtgc tggaaatcct gcgaaccgga atcgatggtc
      961 gagaggcgca gccagtaacc tggtgatcga gcctttgcga ctgaagagca gttggccggc
     1021 gaagattagt caggagttgg ttgtcgatgt ccgaccggtc cagcaaactc gccaccttca
     1081 ttgacggcac caatctccac gccacgacca aagcacttgg atttgatatc gattacaagc
     1141 ggcttcttca ggaattcagg agctgcggga ccttggtacg agcattctat ttcacgatgc
     1201 taaccgaaga tcaggagttc gtgtcgatcc gcccgctgat cgattggctg gactacaacg
     1261 gctacacggt catcacgaag atgagcaagc aattcgtgga tccagccggg catagaaagt
     1321 tgaaaggaga tatcggcgtc gaattggcag tccaagccat ggagctcgcc cagtacatcg
     1381 accagcttat tctattctcg ggcgatggtg ctttccgttc gttggtggca gcgctgcagc
     1441 gtcgcggcgt ccgcgtaagt gtggtttcca cgatggcttg tcagcccccg atggtagccg
     1501 atgagctgcg acggcaagcc gacgtcttca tcgatatcaa ggagttgcag gtgagcgttg
     1561 agcgcgatcc taatcatcgc cagacgcagc gcgattatcg ccacctctcg ccgaacttcg
     1621 ttggaaggca aagccggtcg cattaacgag gcgaggcatt caacaatatg cacatcgaga
     1681 gatttcgcgg ggctagatcg accctcactc cagactagcg cgtgcgatcc tcgcttcaag
     1741 gctttttcca aaaggccgct gatccgacgc cggtgtcgtt cgctgtccaa cctagatcat
     1801 caaataccga caatgcttgc ttgtagagga aagatcgtgc gtgtttgcat aacaatggtt
     1861 tccctggtgg cctttagcgg gccgttgttt ttcttggagc ccgccattgc gcagcaatcg
     1921 aagggccccg cagtcggcat cgtcgaggta gtcaatcgac cgatcatcga gagaacggaa
     1981 ttcttaggac gtatccaggc cgtcgatcgc gtgaacgtcg ttgctcgcgt gactgcattt
     2041 ctggaaaagc ggcttttcaa ggaaggagct gaggtcaagg ccaacgatcg gctctatctc
     2101 ctggaacgcg gacctttcga agctgatctc gaggctaaaa aggcacaggt agcgcagctc
     2161 gaggcgaccc tcgagaacgc gcaactgacg gcggaccgtc agcgaacact cctgagcggt
     2221 cccgccggac tgccagcgaa ctatgacacg gcggtatcga accagaagag cctcgaggcc
     2281 caggtcgagg cagcgaaggc tcaggtcagc ttttcgcaga tcaacctcaa ctataccgat
     2341 atccgctcac ccatcgacgg caaggtcgat cggacctcgg taaccgaagg caacgtcgtc
     2401 tcgccaacct ccggggtgct caccacgatc gtcagccagg atccgatgta cgtggtattt
     2461 cccgtctctg ttcgcgaggc gctatcgttg agggaccgat acgccgccaa aggcggccgc
     2521 aaagcggtcg tgatatcgct caagctgccg gacggccgca tctatggtca gaccgggcgt
     2581 atcgacttcg cgagcaactc ggttaccgag agcaccgata ccttgctggt ccgaggggag
     2641 atcgcgaacc cacttctgtc gccgagcgct tcaaatgcga cccgggagct gacggacggt
     2701 gaactggtca ccgtcatgct cgaaggcgcg cagccggtgc aaacgctcgc gatcccgcgc
     2761 gctgccgtgc tctccgacca gcagggcaac tacgtcctcg tggttggcgc cgagaacaag
     2821 gttgaacagc atcgcgtcaa gctcggacag tccagtcaga ccatcgcatc cgtcatgaac
     2881 ggcctctcgc tcggcgataa agtcatcgtc gaggggcttc aacgggtgaa gccgggacag
     2941 gtagtcgagc cgggtcctgc cagcggcgaa gtccaagcca tgatgaagtc gaccggcggc
     3001 gccgcccaag gcacgggttc gaagggcgag agtgttttgg ggacaagctc cactcagccg
     3061 aatggaggct aacgatgata tcctcgatct tcgtggatcg tccccgcttg gccatcgtga
     3121 tcgcgttcgt gacgagcatc gcgggcctcc tcgcgttgac gcagataccg gtggctcaat
     3181 ttcccgacat cgtcccgccc caggtggtcg tgagcggcac gttccctgga gcctcggcgg
     3241 ccgtcgtcga gg
//
LOCUS       DATAQL010000823         6641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_695905, whole genome shotgun sequence.
ACCESSION   DATAQL010000823 DATAQL010000000
VERSION     DATAQL010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695905"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VHS97_14310"
     CDS             <1..1327
                     /locus_tag="VHS97_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449429.1"
                     /translation="AQAKRRWTTMILGLGLLLIYSANNRELGTDDTVATTLLPLSLAR
                     GDGLSLDRFEKLLREGDEVVPFAVLSRGRIISRYPVAPALLVMPLMVPQIASLDWHEP
                     RWEHTTIRAMTACRRMAKWSSTALMVLAAVIFHRFLLRLGLTRGALPAVVAAFLGSDL
                     WPIASQALWQHGPAALALIAALALLHPGPVSRWRLVLAGIATTLLFTARLLDSLFAAV
                     IVTWIVRSQPRGLIWFLPVPILGAALLLLYNLWYFGTIIGGQAQLEALHPQLHGVTGT
                     WSASLAEGAAGTLFSPNRGLFIFSPWIALALGVAVVPAVARRLASEGLVAWLLAALLP
                     YFLALSKYSVWWGGHCFGPRYWTDVIPLFAIVMAFAFEWIGTHSRALVAFSYMIIAFS
                     IGVHAIGAFCYPSTWNQSPANVDLHHERLWDWRDTELSRCLIESIRGRQ"
     gene            complement(1454..2044)
                     /locus_tag="VHS97_14315"
     CDS             complement(1454..2044)
                     /locus_tag="VHS97_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX3449430.1"
                     /translation="MSKCVLDLPTLVLNRQWQPVHVTNVARSLILLWNDAARVVDPEE
                     YRLFSWTDWAMRPPAHGEPCIRSARLRLPVPEIITLAHYDRLPSTAVTFSRRNVAKRD
                     HYVCQYCGAQPGAESITIDHVLPRAQGGTSSWTNCVAACECCNARKGYRTPDQAGMRL
                     RRRPVRPEWKPFYAAQGARIESWARFLTHEPALALA"
     gene            complement(2280..2825)
                     /locus_tag="VHS97_14320"
     CDS             complement(2280..2825)
                     /locus_tag="VHS97_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449431.1"
                     /translation="MSRKKERKKEHARDAKESSRVEELRQKWLGDEPDVPADAAAPQN
                     GHDVLPSSPPRACCDEGAAGDAGRRVRLTWDEIQEIVRRDLPGHEAVRSETRPAGDFQ
                     PKPQAVSPDLEAIQEKYWPENPSRPVLNSVGSDSIPDEIVVVRLEGSQAPDSPTEPTR
                     GPGLKVAVISGTEMKVIGLQG"
     gene            complement(2929..5544)
                     /locus_tag="VHS97_14325"
     CDS             complement(2929..5544)
                     /locus_tag="VHS97_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3449432.1"
                     /translation="MSGEVADPSAWPGDFAVHLLDVGEDKYGECALCRFGATTVLIDG
                     GHAGDVRGRGDCPSIPDQLAVILGHGPPFRVSLLIVTHAHSDHIGCLPKLVLGSLLRA
                     DWALVTDPGLGWGRSPGDATPDAGSTAGRLAAALREESHADITDDGKLARFLGDVANL
                     ESDYIAMLRSLAYSGTRVVRYRDDQSALAELHEAFAEIGLRVLGPSLEQLLECTELIA
                     RQSSGARDMIAETLRRDASVRDVDAYRELVAGLRSSDAFKKGAAPAVNNQSLITCFEY
                     RRRRLLFTGDMQFAKPEVEGLDGAMAALRESIQKAGPFDFVKLPNHGSYNACDEKLLT
                     QLGARVLGISTGVGDPAHPSPQVLDMLAEHAGLVSWARTDRNGALSITLHEPGTPGVR
                     LTRGKLSDPNPNTADTPPLLPARPSSIAAIPPPATTARGPGVPPVPNVVEPQVTAVPT
                     PPEYVVVRARVPHTTTRVTIRIDVQPEAAELVAPSDRPTSLPSAPSVISQSFPDLRIA
                     AGRRLPPLLFATSRVALRRSIGREEADHALKALSDQSFHVYDGLPDEMPHAADAIALV
                     QKQLSGHPEIQGVVLIGGYDVVPAQTVRVLSDELFRQLPSGLDDPDDFIVWSDAGYGD
                     ICGDGLPEIPVSRIPDGRTPDLVFNCLRAGEATRGRTRFGLRNYYRPFADAIFHELVP
                     GQGRMLTSRHITPGQITQESVAAWDAVYLMLHGDFSDGSRFWGERLPGVMMEAIDLSN
                     CRHRATPVVFSGTCWGALCADLKAMAYSPGVPLPVRTPGSSIALALLQAGAVAFIGCT
                     GAHYSPSSAPYNYYGAPLHEEFWRQYNAGFPPSQALFEAKKEYLKAIPHRSGLVGRAI
                     ETKILREYTCLGLGW"
     gene            complement(6066..6138)
                     /locus_tag="VHS97_14330"
     tRNA            complement(6066..6138)
                     /locus_tag="VHS97_14330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6102..6104),aa:Arg,seq:tct)
BASE COUNT         1171 a         2091 c         2091 g         1288 t
ORIGIN      
        1 agcgcaggcc aagcgtaggt ggacaacgat gatcctcggc ttggggcttc tactgattta
       61 ctcggccaac aatcgggaac tgggcaccga cgacaccgtg gcgacgaccc tgctgccgct
      121 gtcactcgca cggggtgacg gactctcgct cgatcgcttc gagaagctgc tgcgcgaggg
      181 ggatgaagtg gtaccgtttg ctgtcctttc gcgcggacgg atcatctcgc gttatccggt
      241 tgcccctgcc ttgctggtca tgccgctgat ggtgccgcaa atcgcgagcc tggattggca
      301 cgagccccgc tgggaacata ccacaatccg ggctatgaca gcatgccggc ggatggcaaa
      361 gtggtcgtcg accgcactga tggtgcttgc cgcggtgatc tttcaccgtt tcctcctccg
      421 gctggggcta actcgtggcg cgctgcccgc cgtcgtggcc gcatttctgg gctcggacct
      481 ctggccgatc gcgagccagg ctctctggca gcatggaccg gcggcgctgg ccttaattgc
      541 ggccctcgcg ctcttacacc cagggccggt ttctcgctgg cggctggtgt tggcggggat
      601 cgccaccacg ctcctgttca ccgcacgcct gcttgactcg ctgttcgcag cggtaatcgt
      661 cacgtggatc gtccgcagcc agccacgggg cttgatatgg tttttacccg tgcccatctt
      721 gggcgcggcg ttactgctct tgtacaacct ctggtacttc gggaccatca tcggcggcca
      781 ggcgcagcta gaggcattgc acccgcagct tcatggcgtg accggcactt ggtcggccag
      841 cctggccgag ggtgcggcgg gcaccttgtt cagccccaat aggggcctgt ttattttcag
      901 cccctggatc gccctggctc tgggcgtcgc cgtcgtgccg gccgtggcac gccggctcgc
      961 gtcagaagga ctggttgcct ggctattggc tgcgctcttg ccctatttcc tcgccctatc
     1021 gaaatactcc gtgtggtggg gcggacattg cttcgggccg cgatactgga ccgacgtcat
     1081 tcctttgttt gccatcgtca tggccttcgc gttcgaatgg attgggacgc attcgcgcgc
     1141 tctggttgct ttctcatata tgataatagc tttctcaata ggagtgcatg ccatcggcgc
     1201 tttctgctac cccagcacgt ggaaccagag cccggcgaac gtcgacctgc accacgagcg
     1261 tctctgggac tggcgcgaca cggaattgtc acgttgcctg atcgagagta ttcgtggtag
     1321 gcaataactc gattctccca actgggctcg tccggggcgc atctcgctcc gtttggggac
     1381 ctggctgctc gcggctctcc caggagccca cccctgtttg tgttgacgtt tcgaacaagc
     1441 cgggctggct ggctcaggcc agggccagcg ccggctcatg cgtgagaaac ctcgcccagc
     1501 tttcgatccg ggctccctgc gcggcgtaga agggcttcca ctcgggacgg accggccgcc
     1561 ggcgcagccg catgccggcc tggtcgggag tgcggtaccc tttgcgggcg ttgcaacact
     1621 cgcaggcggc cacgcagttg gtccagctcg acgttccgcc ctgcgcgcgc ggtaggacgt
     1681 gatcgatggt gatcgactcg gcccccggct gcgcaccgca gtactggcag acataatgat
     1741 cccgcttggc gacattgcgc cggctaaacg tgacggccgt gctcggcagg cgatcgtaat
     1801 gcgccagagt gatgatctcg ggcaccggca gccgcaaccg tgcggagcgg atgcaaggtt
     1861 cgccgtgggc cggcgggcgc atggcccagt cggtccacga aaacagccgg tactcttccg
     1921 gatcgacaac ccgcgccgca tcgttccaga gcaagatcaa cgaccgggcc acgttagtca
     1981 cgtggaccgg ctgccattgc cggttcaaga ccaaggtcgg tagatccagc acgcacttcg
     2041 acatcatcgc ctccgtaccc aggaaacggt gagggtattc ggcgggagtt gaacccgggg
     2101 ccttgagatt cacattttca tgtaaagacg caccatcccg cagcgacgtt cgcgcctaat
     2161 cgccggattc ttgattgccc ggccgtcggg ctcggccggc gagtgtaaac ccaacgtgct
     2221 aatggcgatc gctaaagact cgacgacatc aagccgcaga acatcgcgga ggctgtcgcc
     2281 tacccttgca agccgatgac cttcatttcc gtacccgaga taaccgcgac cttcaggccc
     2341 ggcccgcgcg tcggttcggt cggtgaatcc ggcgcctgag atccctcgag gcgcaccacg
     2401 acgatctcgt cagggatgct gtccgacccg acggagttca gcaccggcct ggatggattt
     2461 tcaggccagt atttctcttg gatggcctcc agatcagggg agaccgcctg gggctttggt
     2521 tggaaatctc cggccggcct tgtctcggat cgcactgcct cgtgcccggg caggtcgcgc
     2581 cgcacgatct cctggatctc atcccatgtc agccgtacgc gccggcctgc gtcaccggcg
     2641 gcaccctcgt cgcaacaggc cctcggcggg gatgacggta gaacatcgtg gccgttctgt
     2701 ggcgccgctg catcggccgg cacatccggt tcgtccccca accacttctg gcggagctct
     2761 tcaactcgcg acgattcctt ggcgtcgcgc gcgtgctcct tttttcgttc cttctttcta
     2821 gacataaaag tccccaatct ttaacgatct taccagactt tcgtgcaata gcgagcaggg
     2881 aggttcctta cctccgcttt gggggtcttt cttccctgcg gcgtgcggct accaacccag
     2941 gcccaggcat gtatactcgc gcaagatctt cgtctctatc gcccggccga ccagtccact
     3001 ccggtgtgga atcgctttca ggtactcttt cttggcctcg aacagcgcct gggacggcgg
     3061 gaagcccgca ttatactggc gccagaactc ctcgtgcagt ggggccccat aataattgta
     3121 gggcgcggaa gaaggggagt aatgagcgcc ggtgcagcct ataaaagcga ctgcgccggc
     3181 ctgtaaaagc gccagcgcga tcgacgaacc cggcgtccgg accggcaggg gtacgccggg
     3241 cgagtaggcc atggccttca ggtcggcgca gagtgccccc cagcaggtgc cggagaagac
     3301 gacgggtgtc gctcggtgcc tgcaattact caggtcgatc gcctccatca tgaccccggg
     3361 cagccgctca ccccagaacc ggctgccgtc actgaagtcg ccgtgcagca tcaggtacac
     3421 ggcgtcccag gcagcaacgc tttcctgggt gatctggccg ggggttatat gtcgcgatgt
     3481 gagcattcgg ccctgcccgg gcaccaactc gtggaaaatt gcatcggcaa agggacgata
     3541 gtagttgcgg aggccgaagc gcgtcctacc gcgcgtggcc tcgccggccc gcaagcagtt
     3601 gaaaacgagg tccggcgtcc gcccgtcggg gatacgcgag acagggattt caggcagacc
     3661 atcgccgcag atatcaccat aaccggcgtc gctccagacg atgaagtcgt ccgggtcgtc
     3721 aaggccggac ggcaactggc gaaacagctc gtcgctaaga actctgaccg tctgggcggg
     3781 cacaacgtca tagccgccga tcaggaccac gccctggatc tcgggatggc cgctcagttg
     3841 tttctgcacc agcgcgatgg cgtcggcggc atgcggcatc tcatccggca gcccatcgta
     3901 gacgtggaag gactgatcgg agagcgcctt gagggcatga tcggcttcct cccggccgat
     3961 gctccgccgc aacgcgaccc gcgaggttgc gaacaagagc ggagggagcc gtcgcccggc
     4021 cgcgatccgc agatcgggga agctctggct gatcacactc ggagctgaag gcagactcgt
     4081 cggccgatca gacggcgcga cgagctcggc cgcctctggt tggacgtcga tgcgaatcgt
     4141 gacgcgggtc gtcgtgtggg ggacgcgggc gcgtaccacg acgtattcgg gcggagtcgg
     4201 gacggcagtg acttgcggct caacgacgtt tgggaccggc ggaacaccag gcccgcgcgc
     4261 tgtcgtggcc ggggggggaa tcgcggctat gctcgaaggt ctcgcaggca gcaacggcgg
     4321 agtgtcggcc gtgttgggat tgggatcgct gagctttccc cgcgtgagcc ggacgcccgg
     4381 cgtgcctggc tcatgcaagg tgatcgagag ggccccgttg cggtcggtgc gcgcccaaga
     4441 gacaagaccg gcatgctcgg cgagcatgtc gagcacctgg ggactggggt gagccgggtc
     4501 gccgacaccg gtgctgatgc ccaggacccg tgcgccgagc tgggtgagga gcttctcgtc
     4561 gcatgcgtta tagctgccgt ggttcggcag cttgacaaag tcgaaagggc cggctttctg
     4621 gatcgattcg cgcagggccg ccatcgcgcc gtcgagtcct tccacctcgg gcttggcgaa
     4681 ctgcatatcc cccgtgaaga gcagccgtcg ccggcgatat tcgaagcagg tgatcaagct
     4741 ctggttgttg accgcaggcg ccgccccctt cttgaaggcg tcgctggacc gcaagccggc
     4801 gactagttct cggtaggcgt cgacgtcgcg cacagaggcg tctcgtcgta gcgtttcggc
     4861 gatcatgtcg cgggcgccgc tgctttgtcg cgcgatgagc tcggtgcatt cgagcaactg
     4921 ctcgagcgac gggcccagga cgcgcagccc gatctcggcg aaggcttcat ggagctcggc
     4981 caaggcgctc tgatcgtctc gataccggac gactcgcgtg ccgctatagg ccagtgagcg
     5041 gagcatggcg atgtagtcgc tctccaggtt ggccacgtcg ccgaggaacc gcgcgagctt
     5101 gccgtcgtcg gttatgtcgg cgtggctctc ttcgcgcagg gcggccgcga gccggcccgc
     5161 ggtcgacccg gcgtctgggg tggcatcgcc cggactcctt ccccagccga ggcccgggtc
     5221 ggtcacgagc gcccagtcag cgcgcagcag cgagccaagc accagcttgg gcaggcagcc
     5281 gatgtgatcc gaatgagcgt gggtcacgat caaaagggac acccggaacg gagggccgtg
     5341 gcccaggatc accgcaagct ggtccgggat cgaggggcag tcgcctcggc ccctcacatc
     5401 gcctgcatgt ccgccgtcaa tgagcacggt ggtggcgccg aagcggcaca gcgcgcattc
     5461 accatacttg tcctcgccga cgtcgaggag gtggaccgca aaatcccccg gccatgccga
     5521 gggatcggcg acctctcctg acattgctac ctccctggta cccgatcagt tcggtcggga
     5581 cgccctttcc cgatcaccgg actgtaacct tccacgtgga caatcctcat gctagcacga
     5641 acagattgcc atatcaattc taatttctct ataacttttc ggacaaaggg cggttggtcc
     5701 gtagcgcctg atgtcttgct ggagatcaac aacgacttcg gttccaagat ttgaggattc
     5761 tgcaatcctg cctacgaacc ggatgacacc gccgccgatg actggcaaag tcaaactcgc
     5821 ccacctggtg agcgggaatt atataatctt agctctgcat gacgggcaat gacgtgaggt
     5881 gtattcacat tccgagggta aacggaattc agcaggggtg catggatttg aaccctgtcc
     5941 acgagttttg gaggctcgct gttctcccag gagcacaccc ctgtttgttt taattcggca
     6001 gtctgcaaaa agcgggcgct ggctcaggcc gtggccagcg ccggcccctc gctcgcaccc
     6061 aagaacgccc cggccaggac tcgaacccgg aacacctcgt tagaagcgag gcatgatcat
     6121 ccatttcacc accagggcaa atctctggct tccgtcgcca ccacctgtcg tccaactaaa
     6181 aaccccgtgc tttgacgcgg gcctggaaga ctccacgggg ttggcggccg gtggtcaaca
     6241 gcgaccgtag gaaagcgaac tcaccgaccg gtcggcgaat cgcttcgtat tcggattcgg
     6301 ttgtacgaag cgcgcgtcca agtattcccc gtatttcagg ccgattatcg ggccgtcatt
     6361 atcgcatcgc acaaaagggt gacgcgggag agggagaaga tgttcgcgtc agcctcggaa
     6421 tttcttaatc gtggccgcaa tgctcacact agtgtcccgt taacctcgat tgacggtggt
     6481 cttttcgaag gccaatgacg caaagattgc gaactgaact gacaacgacc ctggcgggtt
     6541 gacccgtcaa tcgaaggttg accccacact agcatcgcga ctggtcgccc cggccccaaa
     6601 ccggcatggg atatgcattg tacagtcggg acgacatata c
//
LOCUS       DATAQL010000824         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1153533, whole genome shotgun sequence.
ACCESSION   DATAQL010000824 DATAQL010000000
VERSION     DATAQL010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153533"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VHS97_14335"
     CDS             <1..731
                     /locus_tag="VHS97_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HEX3449433.1"
                     /translation="ITPRTEFEALWTLRALAKGVELDPAQIEAETGVPLSAWQGLLDR
                     MKRARYGVIFFEPSSQNTVDGHQTCHAIFALTRDMNAHARVVCVPLGSRGNAVGADNV
                     LTWQTGYPWGVNLARGYPRFNPGEYTTSQIHERGEADAALIIAGDPMSHVGDNARGHL
                     GRIPTIVLDPVTTATTRVATVVFHTATPGIHTPGTVYRADGVPIPLRAAVTSPLPTDE
                     AILRKIESRVRELKAGADLQRGPL"
     gene            763..1581
                     /locus_tag="VHS97_14340"
     CDS             763..1581
                     /locus_tag="VHS97_14340"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HEX3449434.1"
                     /translation="MSLNLRYHAKTSVPVEIEGVIPDRVGAKSLAEIEKLEIQHGNQR
                     LPLAELFTVGGDPSDGRIDFEGELAGVHYIGHGMSEGAIHVHGDAGRHVGGQMTGGRI
                     EIDGNAGDWLGGEMHGGLIQVSGDAGDWIGAGYRGSKKGMTDGTILIRGNVGSEVGTL
                     MRRGTLIVGGSCGDAAGFNMIAGTILVFGSCGIRPGAGMRRGTIALFGPQPTKLLPTF
                     RSAGSFRPLFLHLIFRELDRLGFPVDHRLLDNELHLSHGDLVALGKGEVWMRTN"
     gene            1592..>2864
                     /locus_tag="VHS97_14345"
     CDS             1592..>2864
                     /locus_tag="VHS97_14345"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HEX3449435.1"
                     /translation="MATEYFKIAGGTVYDPANGIDGQVRDLWIAGDKIVEAPIDSEVR
                     PARTIDARGLVVMPGGVDMHCHIVGSKVNTARKMCPEQKRRGQAVLRTRNTRSGTMGS
                     VPSTFATGYKYAGLGYTTAFDAAIPPLTARHAHEELADTPCVDKGFYTLMGNNHYLMR
                     SIQQGEPHKVKALVGWLLTSSKSYAAKIVNPGGIEAWKSRPSGNVDTVDSTIDHFGVT
                     PRQIIKSVAQAVDELKLPHSVHIHANNLGLAGNWTTTHETMKLLEGHRGHLTHIQFHS
                     YGGGAALEDSFSSQVAPLADYVNSHRNLTVDVGQVVFGETTSMTGDGPVGYFLSKVYK
                     SKWYSSDTELEAGCGIVPIEYKNTSLIHAWQWAIGLEWYLMVDDPWRVVMSTDHPNGG
                     SFLAYPQIIRLLMDRTYRQDMLKSAPRQVREQ"
BASE COUNT          576 a          871 c          864 g          553 t
ORIGIN      
        1 cgatcacacc cagaaccgaa ttcgaggcac tctggactct gcgcgccctt gccaaggggg
       61 tcgagctcga tcccgcgcag atcgaagcgg agaccggagt tcccctgtcc gcctggcagg
      121 gcttgctcga tcggatgaaa cgggcccgtt acggcgtaat ctttttcgag ccgagctcgc
      181 agaacaccgt cgatggtcac caaacgtgtc atgccatctt tgctctgacg cgtgatatga
      241 atgctcatgc gcgcgttgta tgcgtgccgc tggggagtcg cggcaatgcc gtgggggccg
      301 acaatgtttt gacctggcaa accggctatc cgtggggcgt caacctggcg cgtggttatc
      361 cccgcttcaa tccgggcgag tacacgactt cccaaatcca cgagcgcggc gaggccgacg
      421 cagccttgat catcgccggc gacccgatgt cgcacgtggg tgacaatgcc cgcgggcatc
      481 ttggtcgcat tcccacgatc gtcttggatc ccgtgaccac agccacgact cgcgttgcga
      541 cggtcgtctt ccacacggct actcccggaa tccacacgcc cggaaccgtc tatcgcgccg
      601 acggcgtgcc gataccactg cgcgcggccg ttacatcccc gctcccaacg gacgaagcga
      661 tcttgagaaa gatcgagagc cgcgtgaggg agctcaaggc gggtgccgat cttcaacgtg
      721 gaccattgtg acatttttca tttcgtgtga actgtcaaac tcatgtctct caacttgcgc
      781 taccacgcga aaacctccgt ccctgtcgaa atcgaggggg tgatccctga tcgggtaggc
      841 gctaagtccc tggcggagat cgaaaagctc gaaatccagc atggcaacca gaggctgccg
      901 ctggccgagt tgttcacggt cgggggtgat ccctccgacg gtcgaatcga tttcgaggga
      961 gagctggctg gcgtccatta cattggccac ggcatgagtg aaggcgcgat ccacgttcat
     1021 ggcgacgcgg gacggcacgt cggcggtcag atgactggcg gacggatcga gattgatggc
     1081 aacgccggcg attggttggg gggcgagatg cacgggggcc tgatccaagt ttcgggcgac
     1141 gcgggagact ggatcggcgc cggttatcgc ggcagcaaga agggtatgac cgacggcacc
     1201 atcctgattc gcggcaacgt aggaagcgag gttggtacgt taatgcggcg cggtactctg
     1261 atcgtgggag gctcatgcgg agacgccgct gggttcaaca tgatcgccgg caccattctc
     1321 gtctttggat cgtgtggcat ccggcccggt gcgggcatgc gtcgaggtac gatcgcgctc
     1381 tttggccccc aaccgaccaa gcttctcccc accttccggt ccgccggaag cttccggccc
     1441 ctttttctcc acttgatatt tcgcgagctc gaccgtctgg gttttccggt cgatcaccgg
     1501 ctgctcgaca acgaactgca cctttctcat ggcgacttgg tcgctcttgg gaagggcgag
     1561 gtctggatga gaaccaactg acggaaccaa catggccact gagtacttca agatagcggg
     1621 cggaacggtc tatgacccag ccaatggcat cgacggccag gtgcgcgatc tctggattgc
     1681 cggcgacaag atcgttgagg ctcccatcga ttccgaggta cggccagcgc ggacgatcga
     1741 tgcccgcgga ctggtcgtca tgcccggcgg cgtcgatatg cactgccaca ttgtcggctc
     1801 caaggtcaat accgctcgca aaatgtgccc ggagcagaaa aggcgcggcc aagccgttct
     1861 caggacccgc aatacacgca gcggcacgat gggaagcgtg cccagcacgt tcgccaccgg
     1921 ctacaagtac gcagggctgg gttacacgac ggcattcgac gccgctattc cgcctctcac
     1981 cgcccgtcat gctcacgaag aacttgcaga tacgccgtgt gtcgacaaag gcttttatac
     2041 gctgatgggt aacaatcatt acctcatgcg gtcgatccag cagggagagc cgcacaaggt
     2101 gaaagcgctt gtcggctggt tattgacctc gtcgaagtcc tatgccgcca agattgtcaa
     2161 tcccggcggt atcgaggcgt ggaagagccg tccgtccggc aacgtcgaca cggttgactc
     2221 gaccatcgac cactttggag tgacgccacg acagatcatc aagtccgtcg cgcaggctgt
     2281 cgacgagttg aaattgcccc actcagtgca tatccacgcc aacaacctcg gccttgccgg
     2341 caactggacg accacccacg agaccatgaa actcttggag gggcaccgcg ggcacctgac
     2401 ccacatccag tttcatagct acggtggcgg agccgccctt gaagactcgt tctcgtcgca
     2461 ggtcgcgccg ctggccgact atgttaattc ccatcgcaat ctgacggttg acgtgggcca
     2521 ggtcgtcttt ggcgagacca ccagcatgac tggcgacggc cccgtcggct acttcctgag
     2581 caaggtgtat aagtcaaagt ggtacagcag cgacaccgag ctcgaggcgg gttgtggcat
     2641 cgtgccaatc gagtacaaaa acacgagttt gatccacgcc tggcaatggg cgatcggact
     2701 ggaatggtac ctgatggtcg atgatccctg gcgggtggtc atgagtaccg accaccccaa
     2761 cggcggatcg ttcctggcat acccccagat catccgtttg ctcatggacc ggacctaccg
     2821 gcaagacatg ctcaagtccg ctcctcgcca ggtgcgcgaa caga
//
LOCUS       DATAQL010000825         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1001292, whole genome shotgun sequence.
ACCESSION   DATAQL010000825 DATAQL010000000
VERSION     DATAQL010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001292"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VHS97_14350"
     CDS             <1..1283
                     /locus_tag="VHS97_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449436.1"
                     /translation="LAAPSNTDFYSVQPPSLYAGPVTVQLQTSGISFLQPEVQVFNQH
                     FKLLGEAQSTSDLGDVVSVQLPSVNPFQTYYIEVSSPATDAFGTGRYALSVTYNDRSI
                     VNPASLPSILQGPYDSLSAGDLAGLLGDVGNLLFQNGILDLNNTFLTAEPLSSQPGYP
                     SNTQYNVVASLNGPSAVDMYRIQAPQAPAGKTDVLTVSLAQMPVNGVLPVVSVYDANT
                     NPVSAEILLNGNGNYVVQATGLTPGGTYYLQVSPAPAPAPAVGNFSLTASFGLAPAVV
                     QTFVSGTLSASELEDQYTLYVAESQLFQFILSTKASASSTNIEVGMQIYDKTGAVVFS
                     LIGRLGETVSGASILLTPGAYRVTISVVNSSGTAVPSIAYQVSGGSLSDPIGIASSDP
                     AEEPMYPCPGDPSVDCYCYPDGTYSTTPYQASPT"
     gene            complement(1448..>3360)
                     /locus_tag="VHS97_14355"
     CDS             complement(1448..>3360)
                     /locus_tag="VHS97_14355"
                     /inference="COORDINATES: protein motif:HMM:NF019227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HEX3449437.1"
                     /translation="LKAKAGKDPQAQVKLALWCEAHGMNGERVKHLAQAVLSDPKNVT
                     ARGLLGLIALGSRWETADRARERIKADDAHTANRAEYQERRSKLTNDEITSQQAADLL
                     EKKGDYQAAYRARLRSNRRLADAHVELGLWCDRNGLKPEETAHFTLAVHLDPYRDSSW
                     KHLGYVKRNGRWASRDQAADDERDEREQKKADRYWEPLLKKWTDWLGDKRHREEALDL
                     LATVVDRRAVPSILKLFPIDRPEADQLHRVRLLTRIDDPSSSRAIANQAIKTQFSSVR
                     RAAIETLKKRPPRDYAGNLVEMIHGSIRYEVQAVTGPDSRGKLAIEAPRFRMVRTYDV
                     PPAFELASSFRGYVGYDDNGLPVVVQGRELDDMKRQILNPQLIAAKVQEIEIRTANML
                     NQATLAAQVQMAADISAIKRANEQAHDGNASIVPVLKSAAGAPASLGDDEDAWRGWWF
                     DTLGYSYQASPKPTFTQDVVAQYLPYSIRTCFAAGTPVHTLDGARPIEAIQVGDQVIS
                     QDAATGALSFQPVVFVHRNPPAQTLRLVLSDGESVVCSVYHRFWRANLGWTQARELKP
                     GDSLRSLAGIVRVVSIEPDSVQPLYNLDVAGSRTFYVGRTILLVHDNTLPDHRLKPFD
                     TLPVVELTSRPD"
BASE COUNT          584 a         1076 c         1022 g          678 t
ORIGIN      
        1 accttgccgc cccctcgaat accgacttct actcggtgca gcctccctcg ctctacgctg
       61 ggccggttac cgttcagctc cagacctcgg ggatcagctt cctacaacca gaagtgcaag
      121 tcttcaatca gcacttcaag ctgctcggcg aggcccagtc gacgagtgac ttgggggacg
      181 tcgtgtcggt gcaactcccc agtgtcaatc cctttcaaac ttactatatc gaagtttcca
      241 gcccggcgac ggacgctttc gggaccggcc gttacgcgtt gtcggtcacg tacaacgacc
      301 ggtcgatcgt gaaccccgcc agcttgccgt cgattctcca agggccgtac gactccctaa
      361 gcgcgggcga cctagccggg ctgctcggcg acgtgggcaa cctgctcttc cagaacggta
      421 ttcttgacct caacaacacg tttctgaccg ccgagccgct ctcgagccag cccggttatc
      481 cgtcgaacac ccagtataac gtggtggcca gcctcaacgg cccctccgct gttgacatgt
      541 atcgaatcca ggcaccgcag gcccccgccg ggaagacaga cgtcttgacg gtgagcctgg
      601 cccagatgcc ggtcaacggc gtattgcccg tcgtctcggt ctacgatgcg aacaccaatc
      661 cggtgagcgc cgagatccta ctcaatggca acggcaacta cgtcgttcag gccaccgggc
      721 tcacgcccgg ggggacctat tatctccagg tgtcgcccgc cccagcgccg gcgccggcgg
      781 tcggcaattt ctcattgaca gccagcttcg gcctggcgcc cgccgtcgtg caaacgttcg
      841 tctccggcac gttatcggca tccgaactcg aggatcagta caccctgtac gtcgctgaaa
      901 gccaactgtt ccagttcatc ttgtccacca aggctagcgc atcgtcgacg aacatcgagg
      961 taggcatgca gatctacgac aaaacaggcg cggtcgtctt cagcttgatc ggacgtcttg
     1021 gcgagaccgt tagcggcgcc agcatccttc tgacgccggg tgcgtaccgc gtgaccatct
     1081 ccgtcgtcaa ttccagcggg actgcggtgc cttcgatcgc gtatcaggtg agcggaggca
     1141 gtctctcgga ccccatcgga atcgcctcgt cggacccagc cgaggaaccc atgtatccgt
     1201 gcccgggcga cccctcggtc gattgctact gctaccccga tgggacgtat tccaccaccc
     1261 cctaccaagc ctcgccgacg tagcaaatgg cggtgtcgta ggtgtgacga gcgcagcaga
     1321 ttcacgcgat taccgcctcg tccgcaaaaa agggtcagga cgcctatttg ctaaaaaggg
     1381 tcctgaccgc ctttgccgct tggcggccct ttaatgtggc ggtcggcgaa attcaggaac
     1441 ttccgcctca gtccggacgt gacgtcaact ccacgacggg cagtgtgtcg aacggcttaa
     1501 gccggtggtc ggggagcgta ttgtcgtgga cgagtaggat cgtgcggccg acgtagaagg
     1561 tccgtgagcc ggcgacgtcg agattataga gaggctggac cgagtcgggc tcgatgctaa
     1621 cgacccggac gatgccggcc aacgagcgga gggaatcgcc cggcttgagt tctcgggcct
     1681 gggtccagcc gaggttagct cgccagaaac ggtgatacac gctgcacacg acggactcgc
     1741 cgtcggatag caccaaccgc agggtctggg cgggcgggtt gcggtggacg aacacgactg
     1801 gctggaagct caatgccccg gttgccgcat cctggcttat gacctggtcg cccacttgaa
     1861 tcgcttcgat cgggcgggcg ccgtcgagcg tgtgtaccgg cgtaccggcg gcgaaacaag
     1921 ttctgatgga gtacggcaga tactgcgcca cgacgtcctg cgtgaacgtc ggtttggggg
     1981 acgcctgata gctgtagcca agcgtgtcga accaccagcc gcgccacgca tcctcgtcgt
     2041 cgcccaagct cgccggtgca ccggccgcgg acttgagtac cgggacgatg cttgcgttgc
     2101 cgtcatgcgc ctgctcgttg gcccgtttga tggcgctgat gtcagcggcc atctgaacct
     2161 gcgcggcaag cgtggcttgg ttgagcatgt tggccgtgcg gatttcaatc tcctggacct
     2221 tggccgcaat aagctgaggg ttcaagattt gccgcttcat gtcgtcgagc tctcggccct
     2281 gcacaaccac gggcaggccg ttgtcgtcgt agccgacata gccgcggaaa gacgacgcga
     2341 gttcgaaggc gggcggcacg tcgtaagttc gcaccatgcg aaatcggggt gcctcgatcg
     2401 ccagcttacc tcgcgaatcg ggacccgtca cggcctggac ctcatagcgg atggacccgt
     2461 ggatcatctc gaccaggttc ccggcgtagt cgcgcggcgg gcgtttcttc aaggtctcga
     2521 tcgccgcccg gcgcaccgaa ctgaactggg ttttgatcgc ctggttggcg atggcccgcg
     2581 atgatgacgg atcatcgatc ctggtcaaga gacggacgcg gtgaagctgg tcggcctcgg
     2641 gacggtcgat agggaagagc ttcaaaatcg acggtaccgc gcggcggtca acaacggtcg
     2701 caagcagatc tagggcctcc tcgcgatggc gcttgtcacc cagccagtcc gtccatttct
     2761 tcaggagcgg ctcccagtag cgatcggcct ttttttgctc gcgttcatcg cgctcgtcgt
     2821 cagcggcctg gtcgcggctc gcccatcgtc cgttccgctt gacatagcct aaatgcttcc
     2881 agctcgaatc acggtacgga tcgaggtgga cggccaatgt gaagtgggcg gtctcctcgg
     2941 gctttagccc gttgcgatcg caccaaaggc cgagctcgac gtgtgcgtcg gccaggcgtc
     3001 ggttgctcct gagccgggct ctataggcgg cctggtaatc gcccttcttc tccaggaggt
     3061 ccgccgcctg ctggcttgtg atttcgtcgt tcgtcagctt ggaccgccgc tcttgatatt
     3121 cagcccgatt tgccgtatgg gcgtcatctg ccttgatccg ctcgcgggct cggtctgccg
     3181 tttcccatcg gctgcccagt gcgatcaggc ccagtagccc acgggccgtc acgtttttcg
     3241 ggtcggacag caccgcctgt gccaggtgct tgacccgctc gccattcatg ccgtgcgcct
     3301 cgcaccagag cgccagcttt acttgagcct gcggatcctt gccggccttg gctttgagcg
//
LOCUS       DATAQL010000826         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5428710, whole genome shotgun sequence.
ACCESSION   DATAQL010000826 DATAQL010000000
VERSION     DATAQL010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5428710"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VHS97_14360"
     CDS             <1..964
                     /locus_tag="VHS97_14360"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449438.1"
                     /translation="SSTHRRMRRGMLVTTSLAACVVASFGAVVPAQTISREQLIFLTA
                     EWKGERFSDGRPKVPDSVLARMRKVSIEEAWGYMRSHGYPLQFEGDWKTIHDNIPVVG
                     RALTAQYVPARAELAKRMLDRGHQEGRVGAMNSWPIDALQPGDVYVADGFGKIHEGTL
                     IGDNLGNSIFAKSGNGVVFDGSVRDLQGLSEIDGFNAFVRGWDPTAIRDMMLIGLNTA
                     VRIGHVTVLPGDVVLAKREGVIFIPPHLAEAVVNQAERIMLRDRFGHQRLREGKYTPG
                     QIDTGWTKDIEEDFTTWLMQNKDRLDVPRTTIEAMIREHSPRDK"
     gene            1050..2450
                     /locus_tag="VHS97_14365"
     CDS             1050..2450
                     /locus_tag="VHS97_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3449439.1"
                     /translation="MPTTIRNTPQCSPDGDSLTRRGLFKSASVGGLSLAALTHAPLEE
                     QIAYASQNVNRSSKPSDLRITDLRIAEIRGAPMRVPIIRIDTNQGLTGYGEVRDGGSP
                     RYALALKSRLLGENPCNVERLLRRLKQFGSHGRQGGGVSGVEMALWDLAGKAYGVPVY
                     QMLGGRFRDRIRLYADTTISRDRAEYARRLKARLDMGYTFLKMDLGINLLAGQQGVIT
                     APLGFNWSGDRMTPHPFTAIQLTDKAIEHLVEYVVAVREAIGYSVPLAADHFGHFSVN
                     DAIRLGRALMPYQLAWLEDLVPWQYTELWKQITSAIEVPTLTGEDIYLKEEFIKLIDA
                     HAVDLVHPDLASAGGILETKKIGDYAEERGVGMSMHFAGTPISFMANVHCAAATQNVV
                     ALEHHGIDVPWWEDIVIGLEKPLVKAGFVAVPDRPGLGIELNEAVIRAHLVPGESYFA
                     PTDEWNRDRSNDRLWS"
     gene            complement(2550..3665)
                     /locus_tag="VHS97_14370"
     CDS             complement(2550..3665)
                     /locus_tag="VHS97_14370"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEX3449440.1"
                     /translation="MNATFSRRKLLQAAGAVALPMPAPATAMMPGPRHEATDTPKICL
                     EIGAGGLAAGAPTQAGERRVKQLGVDHVIARYPGPIPWQEAPLKELIERLKGHGLTLG
                     NIMITGFPKTLYGRPGRDEEIDKVIQSIRAAGRAGLPVIEYNFYAHRLTEGYYEETGR
                     AGAGLTAFDYDRVKDLPPLEREGAHTLDEMWANVTYFLKAVVPVAEQSGVRLALHPND
                     PPAPTSRGSGQIMSTIAGWKRLIDIVKSPSNGLTFDCGVTRELGEDPVEVCRYLGARD
                     CINHVHYRNVTVRKPYEKYTEVFLDEGDNNMFLVMKELVRQKYPRLIYPEHPRALDYD
                     REHANFRSGYPGGGGYAAYAYNVGYTRAMLQAALSST"
BASE COUNT          785 a         1263 c         1230 g          847 t
ORIGIN      
        1 atcgtcgact caccggcgca tgcggcgcgg catgttggtg accacgtcgc ttgcagcgtg
       61 cgtcgtcgcg agtttcggcg cggttgttcc ggctcaaacc atttcccgcg agcagctgat
      121 ttttctcacg gccgaatgga aaggcgaacg attctcggac ggccggccca aggtacccga
      181 ttcggtcctg gctcgaatgc ggaaggtctc gatcgaagag gcatggggtt atatgcgctc
      241 gcacggctat cctttgcagt tcgagggtga ttggaaaacg attcacgata acatacccgt
      301 tgtgggccgc gcccttacgg ctcaatacgt gcccgcgcgc gctgagctgg ccaaacgcat
      361 gcttgaccgc gggcatcagg aaggccgtgt aggtgccatg aattcctggc ccatcgacgc
      421 tctccagccc ggtgacgtat atgttgctga tggcttcggg aagatccacg aggggacctt
      481 gatcggcgac aatctcggca actcaatctt cgccaagtct ggaaatggcg ttgtttttga
      541 cggctcggtc cgcgaccttc aaggtctatc tgagatcgat ggcttcaacg cattcgtccg
      601 gggatgggat ccaacggcca tccgcgacat gatgctgatc gggctcaaca ccgccgtgcg
      661 gatcggacat gtgacggtgt tacccggaga tgtcgtcttg gccaagcgcg aaggcgtgat
      721 ctttattccc ccgcacctcg ccgaggcggt tgttaaccag gcggagcgca tcatgcttcg
      781 cgatcggttc ggccatcaac gcctgcgcga ggggaagtac acaccaggcc agattgatac
      841 aggttggacc aaggacatcg aagaggattt cacgacttgg ctcatgcaga acaaggaccg
      901 attggacgtg ccgcgcacca cgattgaagc aatgattcgc gaacattcgc cccgcgataa
      961 gtgacgactg gcgatcgtct ccgccatcaa ggtgagacgc tgcttctcta acgatcgagc
     1021 gcttctttga gtctgggagt ccatcggtga tgcccacgac gattcgcaat acgccacaat
     1081 gttcccccga tggcgactcg ctcacgcggc gcggcctgtt caagtcggcc agtgttggag
     1141 gcctttcgtt ggccgcgctg acgcatgcgc cccttgagga acagatcgcc tatgcgtctc
     1201 aaaacgtaaa tcggagctcg aaaccctccg acctccgaat caccgatctc cggatcgccg
     1261 aaattcgcgg tgctcccatg cgggtgccga tcatccggat cgacaccaac cagggcctca
     1321 ctggctacgg cgaagttcgc gacgggggca gtcctcgcta cgcgcttgcg ctcaagagtc
     1381 gtctcttggg tgagaaccct tgcaatgttg aacgcctgct ccggcggctc aaacagtttg
     1441 gttcccacgg ccggcaggga ggcggtgtca gcggcgtgga gatggccctt tgggatcttg
     1501 ctggcaaggc ctacggtgtg cccgtatacc agatgctcgg cggtcgcttt cgcgatcgga
     1561 tccgcctcta tgccgatacg acgatttcgc gtgaccgcgc cgagtacgcc cgccgactaa
     1621 aggcccggct cgacatgggc tatacgtttc tcaagatgga tctgggcatc aacctgctcg
     1681 cgggacagca gggcgtgatc accgctccgc tgggtttcaa ttggtcaggc gaccggatga
     1741 cgcctcaccc atttacagcg atccagctca cggacaaggc gattgagcac ctcgtcgaat
     1801 atgtcgtggc cgtccgtgag gccatcggct actctgttcc gctcgccgcc gaccacttcg
     1861 gccacttctc ggtcaatgac gcgattcgcc tcggcagggc actcatgccc taccaactcg
     1921 cctggctcga ggacttggtt ccctggcagt acaccgaact ttggaagcag attacgagcg
     1981 cgattgaggt tcccacgctt acgggagaag atatctatct gaaagaagaa tttatcaagc
     2041 tcattgatgc gcacgcagtc gacctcgtcc atcccgatct ggccagtgca ggggggatcc
     2101 tcgagaccaa gaagattggc gattacgccg aggaacgcgg cgtcggcatg tccatgcact
     2161 tcgccggcac gccaatctcg ttcatggcca acgtccactg cgctgccgcg acccagaatg
     2221 tcgtcgctct cgagcaccac ggcatcgatg tgccctggtg ggaagacatc gtgatcggcc
     2281 tcgagaaacc gctggtcaag gccggcttcg tcgccgttcc cgatcgaccc ggtctcggca
     2341 tcgagctcaa cgaagcagtg attcgagccc acctggtccc cggcgagtca tatttcgcac
     2401 ccacggacga gtggaatcgg gaccgctcca acgaccgtct ctggagttga actccgagcg
     2461 ccgaaggcgc cgggaaactc tgcgcccgtg gaaagctgga aaatcgctct gccgacaacg
     2521 aaagcccgat tctaacaagg cggccaggct caagttgacg acagggccgc ctgcaacatg
     2581 gcccgtgtgt accccacgtt atacgcgtac gccgcatatc ccccgccacc cgggtacccc
     2641 gatcggaagt tggcgtgttc gcggtcgtaa tcgagagcgc ggggatgctc ggggtaaatc
     2701 aggcgcgggt atttttgccg gaccagctct ttcatcacca ggaacatatt gttgtcgcct
     2761 tcgtcgagga acacctcggt gtacttctcg tagggctttc gtaccgtcac gttgcgatag
     2821 tggacatggt tgatacagtc gcgtgccccc agatagcggc aaacctcgac cggatcctcc
     2881 cccagctcgc gggtcactcc acaatcgaaa gtgaggccgt tcgaggggct cttcacgatg
     2941 tcaatgagcc gcttccaacc tgcgatcgtg ctcatgattt ggcccgatcc tcggctggtc
     3001 ggagccggag gatcgttcgg atgcagtgcc aggcgcactc ccgactgctc ggccacggga
     3061 accaccgcct tgaggaagta cgtgacgttg gcccacatct cgtccagtgt gtgggcgcct
     3121 tcacgctcca gcggaggcag gtctttgacc ctgtcgtagt caaaggctgt caatccagcg
     3181 ccggcccgac cggtttcttc gtaatagccc tcggtcaggc ggtgcgcgta gaagttgtat
     3241 tcgatgaccg gtagacctgc gcggcccgcc gcgcggatcg actgaatgac cttgtcgatc
     3301 tcttcatctc gtccgggccg tccatagagg gtcttgggga atccagtgat catgatgttc
     3361 cccaacgtca gaccgtgacc tttcaggcgt tcgatcagct ccttgagcgg ggcctcctgc
     3421 cacgggatgg gtcccggata cctggcaatc acatgatcca ccccgagctg cttcacccga
     3481 cgctccccag cttgggttgg cgcgcccgct gcgaggccgc ccgccccaat ttcgaggcag
     3541 atcttgggtg tgtccgtggc ttcatgccgg gggccgggca tcatcgccgt tgccggggcg
     3601 ggcatcggca aggccaccgc gcccgcggcc tgcaggagct tccttcgaga gaacgtggcg
     3661 ttcatggtct aagtttcctg tcaccggcat ttatcccgcc ttggcctctt ggatcgcact
     3721 ccagggcgtc gggtcaggaa gaccggtact tgttcacaac agagacaaac cgccgcctaa
     3781 actattccag gcgctcaccg aaaagggggc tgctcgcagt ccttcgacgt gcgcgtcctc
     3841 ccactcgtgt gctccaccga gagaataatc gcgcgccaag cttggcgaaa cgctctactg
     3901 gtttcgtcgc ggcgaaagct cctggattgg cgcatggaga tgaagggaac atgcgttgcc
     3961 tggtatgatc gagaaagcgt gaagggaacg gacacgcgct gtgtttaggc tgaggagcat
     4021 tgggatgttg aaagtcgaac ccgccgcgcg ggggttggat cgcatttcaa cggatgccac
     4081 cgtctcgctc attctcgact ttctggaatg gctcggtccc gagcc
//
LOCUS       DATAQL010000827         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4970042, whole genome shotgun sequence.
ACCESSION   DATAQL010000827 DATAQL010000000
VERSION     DATAQL010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4970042"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1072)
                     /locus_tag="VHS97_14375"
     CDS             complement(56..1072)
                     /locus_tag="VHS97_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3449441.1"
                     /translation="MFRLVNDGSDGSSALPERAATPANEQDALDAYSRVIVTVAETLG
                     PAVVNLRGVAGEGGRRATGSGSGFLFTPDGFLLTNHHVVRGSSRVRVRLSDGREVSGR
                     VIGADPWTDVAVVSADASGLPHAQLGESARLRVGQLVVAIGSPFGFESTVTAGVVSAL
                     GRTLRSITGHLVDNVIQTDAALNPGNSGGPLLDSSGRVIGINTAIIQSAQGICFAIPI
                     NMAKRIIPQLMQHGRVVRGYLGMHGRDVPIARPVARSFDLEQQTAVEVMALEPDGPAA
                     QAGMSDGDLIIALGDQPISTIDDLQNRLADIPVGIPTEVVLLRGNRRLGRFVVPGEYP
                     SLEV"
     gene            1280..2290
                     /locus_tag="VHS97_14380"
     CDS             1280..2290
                     /locus_tag="VHS97_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX3449442.1"
                     /translation="MKAFQFHEFGGPDALRCEDVPNPTPGPGEVLIRVAAVSLNYRDL
                     MMCKGAYNPKLKLPIIPVSDGAGEVVATGAGVTRFKPGDKVAAAFMPDWVNGPPDEEN
                     TRTTLGGGGVGLLAEQAVLPEHGLVPIPAHLSALEAATLPCAGVTAWNALFENGRVKP
                     GDTVLVQGTGGVSIFALQFARLAGAHVIATSSSDQKLARARELGATDGINYKTNPDWD
                     KRARALTGGKGVDQIVEVGGAGTLGKSLRAVRIGGYIALIGVLSGANAEVNPMPVLMK
                     SIRVQGIFVGSRAMFEAMNRAIVASQLRPVVDRVFEFEQAPQALKYLESGAHFGKIVI
                     QI"
     gene            complement(2307..>2766)
                     /locus_tag="VHS97_14385"
     CDS             complement(2307..>2766)
                     /locus_tag="VHS97_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX3449443.1"
                     /translation="GTPQELYNRPHDVFVARLLGPTNLLQGQVESPAADSKGEVVVRT
                     PLGRLIGQMIMRQAPQGAPVTVSIRPETVSLGPAIPSGWNRFPATVERIIFKGELRRF
                     QARGPGDWPIAVAAIQSQSHHVREGQSLTLAVAPEHVVVLPGKFAVASPP"
BASE COUNT          538 a          863 c          867 g          498 t
ORIGIN      
        1 ttgtcacctg cgaagatgct gaatttgaac catgcccacg agggcgacag cgtgatcaga
       61 cctcgaggct ggggtattct ccgggaacga caaagcggcc cagccggcga ttaccgcgta
      121 acagaacgac ctcggtcgga attcccaccg gtatatcggc gagccggttt tgcaggtcgt
      181 cgatggtgga tatcggctgg tcgccaaggg cgatgatcag gtcgccgtcg gacatgccgg
      241 cctgggcagc cggtccatct ggctcgagcg ccatcacctc gaccgcagtt tgttgctcca
      301 ggtcaaagct gcgggcaaca ggccgtgcga tcgggacatc ccggccatgc atgccaagat
      361 agccgcgcac cacccggccg tgctgcatga gctggggaat aatgcgcttg gccatgttga
      421 tcgggatcgc gaagcaaatc ccttgcgccg actgaatgat ggccgtgtta atgccgatca
      481 cgcgaccaga gctgtcgaga agcgggcccc cgctatttcc tgggttcaag gcggcatcgg
      541 tctggatcac gttgtcaacc aggtgtccgg tgatgctgcg cagggtacgg cccaatgcgc
      601 tcacgactcc ggccgtcacc gtcgattcga agccgaacgg gctaccgatc gcaaccacga
      661 gctggccgac gcgcaggcgt gccgattcgc cgagctgggc atgcggcaat ccggaggcgt
      721 cggcggaaac cactgcgacg tcggtccagg gatcagcgcc gattacgcgg cccgagacct
      781 ctcggccatc actcagcctg acgcgaacac ggctcgagcc gcgcaccacg tgatggttgg
      841 taagcaaaaa gccatctggc gtgaacaaaa accccgaccc cgatccggtc gcccgccgcc
      901 ccccttcgcc ggcgacgccg cgcaggttga cgaccgcggg ccccagtgtc tcggcgacag
      961 tgacgatcac tcgcgaatag gcgtcgaggg cgtcctgctc gttcgccggg gtcgccgcac
     1021 gctccggcaa agccgaagaa ccgtcgcttc catcgttaac caaccgaaac atgaatgggt
     1081 tcatctgatt cgacctctgc tcaactgaga tccctggtca acgatcattg tagatggtgt
     1141 ggtcaagtcg ccatcaaaat ggcccgtgca attacgagcc tcttgccgat ctcaccataa
     1201 tgagtgacaa attgagcgtg gtccgaccag ggctccctgg actcaacaac atgtattaag
     1261 ggaggcgaag ggagactcga tgaaagcgtt tcagtttcat gagttcggcg gtccggatgc
     1321 tctacgctgc gaggatgtgc ccaatccaac tcctggcccg ggcgaggtct tgatccgtgt
     1381 cgctgccgtt tcgctcaact accgcgacct tatgatgtgc aagggcgcgt acaatcccaa
     1441 attgaaacta cccattatcc ccgtctccga cggggcgggc gaggtggtgg ccaccggcgc
     1501 gggcgtcaca cgattcaaac cgggcgacaa ggttgccgcc gctttcatgc cagactgggt
     1561 gaacgggccg cccgacgaag agaacacccg aacgaccctt ggcggcggag gcgtcggctt
     1621 gctggccgag caggccgttc tccccgagca cgggctcgtt cctatccccg ctcatcttag
     1681 tgcgctagag gccgccaccc tgccgtgtgc aggagtaacc gcatggaacg ccctgttcga
     1741 aaacgggcgt gtcaagccgg gcgacactgt cttggttcag gggacgggcg gagtctcgat
     1801 cttcgcgctc cagttcgccc ggctcgccgg cgcgcatgtg attgcaacct cgagcagcga
     1861 ccagaaactg gcacgggcac gggagctcgg cgctacggac ggcatcaact acaagacaaa
     1921 ccccgactgg gacaagcgag cccgtgcgct caccggcgga aaaggggtcg accagattgt
     1981 cgaagtcggc ggcgcgggaa ctctcggcaa gtcgctgcgt gcggtccgga taggaggcta
     2041 catcgcgttg atcggtgtct tgagtggggc gaatgccgaa gtcaatccga tgccggtgct
     2101 catgaaaagc attcgtgtcc agggtatctt cgtgggttcg agagccatgt tcgaggcgat
     2161 gaaccgcgcg atcgtagcca gtcagttgcg ccccgtcgtc gaccgcgtct tcgaattcga
     2221 gcaagcgcct caggcgctca agtatttgga aagcggtgct cactttggca agatcgtgat
     2281 tcaaatctag ccttcaccct gttttgtcag ggcgggctgg cgacggcaaa cttgccgggc
     2341 aagacgacga cgtgttcggg cgcgaccgcg agcgtcaggc tttggccttc ccgcacgtga
     2401 tgcgactgac tctggatcgc ggcgacggca atcggccaat cgcctggtcc gcgtgcttgg
     2461 aaccgccgaa gctctccctt gaagatgatt cgctcgacgg tcgccggaaa ccggttccag
     2521 cccgatggga tcgccggacc cagcgacacc gtctcgggcc ggatcgaaac ggtcaccggc
     2581 gccccttgcg gagcctgtcg catgatcatc tggccgatca gacgacccag cggggttcgc
     2641 accacaactt cgcccttgga gtcggccgcc gggctctcga cctggccctg caacaggttg
     2701 gtcgggccca gcaagcgggc cacaaagaca tcatgcggac gattgtagag ctcttgcggc
     2761 gtgccc
//
LOCUS       DATAQL010000828         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1610859, whole genome shotgun sequence.
ACCESSION   DATAQL010000828 DATAQL010000000
VERSION     DATAQL010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610859"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..885)
                     /locus_tag="VHS97_14390"
     CDS             complement(67..885)
                     /locus_tag="VHS97_14390"
                     /inference="COORDINATES: protein motif:HMM:NF025713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEX3449444.1"
                     /translation="MTDLVKRTTRGIFRDLMTNSTVGAISTAFQDEGFAADPDCRYED
                     SSVRRQTTQSYLDAVDWTDPRHVGRFLRVAERLLNGWEPQGLSQFHQSLRRDGYQVDE
                     QTGQITPAGPQLSIESIARLADPSAIREGFDRIRRAISDDPALAVGSAKELIESTAKV
                     VLSERGQLFDDKADLPKLARAAQLSLGLDPSTGSGPDGSDGVKRILGAVTTIANGLAE
                     LRNRGLGTGHGPATARVGLGVRHAHLAVNAALTWCQLMLDTLADPDAPWRKGHS"
     gene            complement(882..1493)
                     /locus_tag="VHS97_14395"
     CDS             complement(882..1493)
                     /locus_tag="VHS97_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3449445.1"
                     /translation="MAELKIGYARVSTVDQDLTAQREALTMLGVDPKRIYVDHGLTGT
                     NRDRPGLRQALAACRSGDTLVVTKLDRLARSVPDARDIVDELTANGVRLNIGGSVHDP
                     SDPVGRLLFTTLSMIAEFEADLARARTREGMAVARAKGRLRGKQPKLSPRQEAHLVEL
                     YRAGGHTIGELEELFPVTRSTIYRAVARAGGRTTGTSIQSTTR"
     gene            complement(2053..2220)
                     /locus_tag="VHS97_14400"
     CDS             complement(2053..2220)
                     /locus_tag="VHS97_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449446.1"
                     /translation="MTFRQRRAVHLLRTSDRTLDEIASLVGYADSVTLRTLLRRRLGR
                     SVREIREHLPA"
BASE COUNT          411 a          797 c          876 g          481 t
ORIGIN      
        1 acgagttggg gtcgccttag taacagaacg aatctggttg ttttcgtgat cttcgtttgg
       61 tcgcggctac gaatgacctt tgcgccaggg cgcatcgggg tcggcgaggg tgtcgagcat
      121 gagctggcac caggtgagcg ccgcgttgac cgcgaggtgc gcgtgccgga cgccgagacc
      181 gacgcgtgcg gttgccggcc cgtgtccggt gcccagcccg cggttgcgca gctcggcaag
      241 tccgttggcg attgttgtga ccgcgcccag gatgcgctta acgccgtcgc tgccgtccgg
      301 tccggacccg gtcgaagggt ccagcccgag cgaaagctga gctgcgcgag ccagtttggg
      361 caggtcggcc ttgtcgtcga atagctggcc ccgttcggag aggacaacct tggcggtgct
      421 ttcgatcagt tccttggcgc tgccgaccgc gagcgcggga tcgtcgctga tcgcccgcct
      481 gatgcggtcg aagccctcgc gaatggctga cgggtcggcg agtctcgcaa tcgattcgat
      541 cgacagctgt ggcccggctg gagtgatctg gccggtctgc tcatcgacct ggtagccgtc
      601 gcggcgtagc gattggtgaa actggctgag gccctgaggt tcccagccat tgagtaaccg
      661 ttcggcgacc cggagaaatc ggccgacgtg tcgtgggtcg gtccagtcaa ccgcatccag
      721 gtacgactgc gtggtctgcc gacgcacgct gctgtcttca tagcggcagt cgggatcggc
      781 cgcgaagcct tcgtcttgaa acgcggtgct gatcgcgccg accgtgctgt tggtcatcaa
      841 gtcgcggaag atgccgcgtg tagtgcgctt tacgagatcg gtcatcgcgt tgtgctctgt
      901 attgacgtcc cggttgttcg cccgccggcg cgcgcgaccg cccggtagat cgtcgagcgg
      961 gtgaccggga acagttcctc gagctcgccg atggtgtgtc ctccggcgcg gtagagctcg
     1021 acgaggtgcg cctcctgccg cggactgagc ttgggttgct tgccacgcag ccggcccttg
     1081 gcccgggcga cggccatgcc ctcccgagtc cgggcacggg caaggtcggc ttcaaactcg
     1141 gcgatcatgc tgagagtggt gaacagcaac cggccgaccg ggtccgacgg gtcgtgcacc
     1201 gaaccgccga tattgagacg cacgccgtta gcggtgagtt cgtcgacgat gtcgcgggcg
     1261 tccggaaccg accgggcgag ccggtcgagc tttgtcacaa cgagcgtgtc gccgctgcgg
     1321 caggcggcga gcgcttggcg caatccgggc cggtcgcgat tcgtaccggt caggccgtgg
     1381 tcgacataga tccgcttcgg gtcgacgcct agcatcgtga gcgcctcccg ctgcgccgtg
     1441 agatcttgat cgaccgtcga cacccgggcg tagccgatct tcagctcagc catgcgaaca
     1501 gtatgccggt tagcccccct tcaccgggaa ccatgaccga accagtctta cgggacgtcg
     1561 gcactccggt ccggctgggc ctcggtcgtg cggcgtgggc gtgccgtttg aggttcccgt
     1621 atcgggcact gttttgccgg gtggtccatc tcagtcgagg gctcgacgcg ctccccatgc
     1681 ggcgtcggca agtggctgct gcgcacatcc ggcctagcgc taaccgccga ggagttggct
     1741 gccgcctacg tgcgaggatc cgcatacctc tcttgtcgcg ccggcgccgg catgtgaggt
     1801 tgctaggaag ttggaaacct atacccgtag cgcgtgtgtg gacgcgccgc taggccactt
     1861 gacccccagt aaatagcggc aacgcatatc cccaggtggc gcgcgttatc tagccgccta
     1921 gcgtgcgtgc atagtgcgca tagacgcgca ggtcgttaac tcacgcaccc gacgtgtggt
     1981 cccaacgtgg tccccaagac ccggcggggc cccacggtgt gcgcgtccca ggtctctaac
     2041 ggacccgcag tatcaagccg gcaaatgctc gcggatttcg cggacgctac ggccgaggcg
     2101 tcggcggagg agggtccgca aggtgacgct atcggcgtag ccgaccaatg aggcgatctc
     2161 gtcgagggtc cggtcgctgg tgcgcagcag gtgtacggcc cgccgctggc ggaaggtcaa
     2221 cgcgccgcac ctcgtcgcgc tcgtccgtgc gggcgcggag ttcaggaacg gcaagctggt
     2281 cgagcgatcc gaaacggaca atcaggcagg aatagctgcc gacgttgagg tcgccgcagc
     2341 tgcctaagaa aagttgatcc acaggtcttg acaataactc caagctagga cgagatcgcg
     2401 aatagtggat cccgctagat cctgcggcac gatgccacca ccgataggga agtcgcccgt
     2461 tagctcaatc tttcacactg ctgaggcctg tgtccgaacg agccgtttag cggtgcgctt
     2521 ggtggctgag caccgcggtg agtacgatcc gttccatcgc cgcct
//
LOCUS       DATAQL010000829         4591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_237928, whole genome shotgun sequence.
ACCESSION   DATAQL010000829 DATAQL010000000
VERSION     DATAQL010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237928"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1246)
                     /locus_tag="VHS97_14405"
     CDS             complement(125..1246)
                     /locus_tag="VHS97_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3449447.1"
                     /translation="MSCFLRKTIAVLALFCPAGAAFADPPGLQTLPIGAAAPDFRLPG
                     VDGKTYSLSEFADAKILFVIFTCNHCPTAQAYEGRLARLQADYKDKGVAVVAISPNNP
                     AALRLDELGFTDVGDSFEDMKYRAKDHNFTYPYLYDGDTQSTAQAYGVLATPHVYIFD
                     RERKLRYVGRFDDAEVKAVKSHDAKNALDALLAGKPVPKETTRVFGCSTKWAEKEADA
                     RKSIAKWDTEPVAIEPVDLAAVAKIAKNDTNKLTLVNLWATWCGPCVAELPELVTMNR
                     MYRKRNFQLVTISLDELSKQDDALKVLKENHLAAKNFILSSDDRDKFAEALDRDWPGP
                     VPYTVLIAPGGKILYRKTGAFDPLELKRAIVSYVGRTSS"
     gene            1489..3420
                     /locus_tag="VHS97_14410"
     CDS             1489..3420
                     /locus_tag="VHS97_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449448.1"
                     /translation="MPQLRWGPGPVFVYESIVATRRWQLYALRFLFVLGLLSALLMGW
                     YMTGFMSRAAGVNAGGLTMRQLSSLGQYFYYAIATTQLAIVLVVAPAATAGSICLDRA
                     RGTLTHICATDLTDWEIVLGKLGARLLPVLSLVVATVPVLALAGLLGGVIIEAIVTLT
                     MITLTLAVFGCTLALAISVRATKTQEVLMAVYLIEGVWVMGPLIWALLAQTGVFPGAP
                     DWFMAINPFVLAWAPYAWPNYLGTGQVAAILAGTLALSAGFVVYAVFRLRVELRRQSG
                     LLITRRSSWLERAGSRLSEWRRRPSLDNDPLLWREWRRGRPSRLARVVWTLYIGLALA
                     GTGWGLFVLADDFGNGSEFLLFVNGFQATFGLLLVSLDAPTVLAEERVRGSLDVLLAT
                     PLSTGRIVLAKWWGAYRHVPALALLPAVGAVFIAASEPNFPIRPGPLRQSTVPLGLID
                     RIAHVATPLAMLLAQGAVIAGVGLALATWIRRIGRAVAVSVASYSFVAFGWLVLLEMG
                     VVTGVLSWFGFFQPDDHNAEQFVAQLAAIACPLGGQMFPFEIIDMAPGESRLRLAFYL
                     GETVVFLVTILIALVILALTLATFDRCMGRASERPRRGPRAPRHKKSKNPSPHSQVFD
                     GRQAVITSMNLAVAQPKRL"
     gene            complement(3377..4072)
                     /locus_tag="VHS97_14415"
     CDS             complement(3377..4072)
                     /locus_tag="VHS97_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3449449.1"
                     /translation="MSILRHAANASLVILFATIALSQAERPAQSASSSSDWRPLFDGR
                     TLEGWEHVGPGKFVVEDGVLRTEDGMGLLWYAREKLGNCVIRVVYKTANVRSNSGVYL
                     RIADKPKDAWYAVHHGFEIQVMDQSTSGRGTGSIYTFAKAAGNPPKSGEWNTLEITLK
                     GNRISTAINGVQAADFDSSELKPDAPEISGEGDPARGPRPESGYIGLQNHDKNSIVYF
                     KDVSVGPLPGSSK"
     gene            complement(4153..>4591)
                     /locus_tag="VHS97_14420"
     CDS             complement(4153..>4591)
                     /locus_tag="VHS97_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3449450.1"
                     /translation="DAFRAHRVGFVFQSFYLMPTLTALENIQIPMFEGPWPPSQRGER
                     ALRLIEEVGLVDRRDHRPGQLSVGQRQRIAIARALANDPQLLLADEPTGNLDSKSQDE
                     IIHLLHRLRDERRLTLVIVTHSHDVARAADRRIPMKDGQIQPE"
BASE COUNT          802 a         1304 c         1487 g          998 t
ORIGIN      
        1 gtaggagcag atcgagcggt tcacaaatca ttccaccccc aacgacctgc tcgattacga
       61 agatcgccag ccatagccgc acgtttagat gggcgatcca attgactcgc cgacggcctg
      121 aacctcacga agatgttctg ccgacatagc tcacaatcgc ccgcttgagc tcgagcgggt
      181 cgaacgcgcc tgtcttgcgg tagaggattt tgccgccggg cgcgatcaaa acggtgtagg
      241 ggacgggacc aggccaatcc cggtcgagtg cctcggcgaa cttgtcgcgg tcgtccgagc
      301 tcaagatgaa gtttttcgcc gcgaggtggt tttccttgag aaccttcaag gcgtcgtctt
      361 gcttggacag ttcgtccagg ctgatcgtca cgagctgaaa gttccgcttg cgatacatgc
      421 ggttcatggt taccagctca ggaagttctg caacgcaggg gccgcaccag gtcgcccaca
      481 ggttgacgag agtaagcttg ttggtgtcgt tcttggcgat cttggccacg gcagcgaggt
      541 caaccggttc aatcgccacg ggctcggtgt cccactttgc gatcgacttg cgcgcgtcgg
      601 cctctttttc ggcccacttg gtcgaacagc cgaaaacgcg cgtggtctcc ttcggcacgg
      661 gtttgccggc cagaagtgca tccagggcgt tcttggcgtc gtgcgacttc acggccttca
      721 cttccgcatc gtcgaatcgg ccgacgtagc gaagcttgcg ttcgcggtcg aaaatgtaga
      781 cgtgaggcgt ggccaaaacg ccgtatgctt gcgctgtcga ctgggtgtcg ccgtcgtaaa
      841 ggtatgggta ggtgaaattg tgatctttgg cacgatattt catgtcttcg aatgaatcgc
      901 cgacgtcggt gaagccgagc tcgtcgagcc ggagcgcagc cgggttattg ggcgaaatcg
      961 ccacgacggc cacccccttg tctttataat cggcctgaag ccgggcgagc cggccttcgt
     1021 aggcctgtgc ggtcgggcaa tgattgcagg taaatatgac gaagaggatc ttggcgtcgg
     1081 caaactcaga gagggagtag gttttgccat cgacgccggg cagccggaaa tcgggggcgg
     1141 ccgcgccgat gggcagagtt tgcaaaccag gggggtcggc aaaagcggcg ccagcgggac
     1201 aaaagagggc gaggactgcg attgtcttcc tcaaaaagca gctcattgtg ggcgtctcct
     1261 cggaatggag aatgatggtg gttggagctg gtagtctgcc agtcgcgacg ctctatgcca
     1321 agtggtttgt cactttgatc gcacgccggg tgcttcttgg gtgccggggt cgatcgctag
     1381 cgcagccgac caagtggttg tcagccgtca tgctggtctg gtacgttcgc agtctactcg
     1441 acaccttcgg aataggggct gcgcggatcg caacaaggag ggacgctgat gccgcaattg
     1501 cgctggggac ccggaccggt cttcgtatac gagtcgatcg tcgcgacgcg gcggtggcag
     1561 ctctacgctc tgaggtttct gttcgtgctt ggccttctgt cagcccttct catgggctgg
     1621 tatatgaccg gctttatgag ccgagctgcc ggtgtgaatg ccggcggact gacgatgagg
     1681 cagctttctt cactcgggca gtatttttac tacgccatcg cgactacgca gctcgccatc
     1741 gtactcgtgg tcgcgccggc cgcgactgcc ggatcgatct gcctggatcg tgcccggggt
     1801 acgttgaccc acatctgcgc caccgatctg accgactggg agattgtgct gggcaagctg
     1861 ggcgcgcggc ttctgccggt gctgtcgctc gtcgtcgcga cagtgccagt gctcgctctt
     1921 gccggtcttt tgggcggggt catcatcgag gcgattgtca ctctgaccat gatcaccctg
     1981 acgctggcgg tgttcggttg cacgctggcg ctggcgatat cggtccgcgc caccaagacg
     2041 caggaggtct tgatggccgt gtacttaatc gagggcgtct gggtcatggg ccctttaatt
     2101 tgggcgctgc tggcgcagac cggagtgttt ccgggggcgc cggattggtt catggcaatc
     2161 aatccgttcg tgctggcctg ggccccttac gcctggccga attatctggg tacgggccag
     2221 gtcgcggcga ttcttgcggg aacgctcgct ctctcagccg ggttcgttgt ttacgcggtg
     2281 ttccggttgc gagttgaact caggcgacaa tcaggattgc tcatcacacg acgatcgtcg
     2341 tggttggaaa gggccggatc gcggctctca gagtggcggc ggaggccgtc actggataac
     2401 gaccctttgc tgtggcgcga atggcggcgc ggccgcccat cgcggttggc tagggtcgta
     2461 tggacgctgt atatcggtct ggcgcttgca ggaaccggct ggggactctt cgtgctcgcc
     2521 gacgatttcg gcaacggctc ggagttcctg ttgttcgtca acgggtttca ggccaccttc
     2581 ggtctgctgc tggtcagtct ggacgccccc acggttctgg ccgaagaacg ggtgagaggc
     2641 agcctcgacg tcctgctggc gacaccgctt tcgaccggac gtatcgtcct ggcgaaatgg
     2701 tggggggcat accgtcatgt gcccgctctg gctttgctgc cggcagttgg ggctgtcttc
     2761 atcgcggcct cggagccgaa tttcccgatc cgtccgggtc cgctccggca atcgacggta
     2821 cccctgggct tgatcgaccg gattgcgcac gtcgccacac cgctggccat gctgctcgcg
     2881 cagggggccg taatcgcggg cgtcgggcta gccctggcga catggattcg ccgcatcggc
     2941 cgggctgtcg ccgtcagcgt cgccagttac tcgttcgtcg ccttcggctg gcttgtttta
     3001 ttggagatgg gagtggtcac gggagtttta agctggtttg gctttttcca gccggacgat
     3061 cacaacgcag agcagttcgt cgcgcagtta gccgcgattg cgtgtccgct gggaggccag
     3121 atgttcccgt ttgaaatcat agacatggcg cctggcgaaa gccgtctccg tctcgccttc
     3181 tacctcggtg agaccgttgt ctttctggtg acaattctga tcgctcttgt gatcctcgca
     3241 ttgacgctcg cgacctttga ccgatgtatg ggccgcgcct ccgagcgacc tcgccgcggg
     3301 ccgcgagcgc ctcggcacaa gaaaagtaag aatcccagtc cgcattcgca agtcttcgat
     3361 ggacgtcaag ccgtgatcac ttcgatgaac ctggcagtgg cccaaccgaa acgtctttga
     3421 aatacacgat ggagttctta tcgtgattct gaaggccgat gtaacccgac tcgggtcgag
     3481 gcccgcgcgc gggatcgcct tcgccggaaa tctcaggcgc atcgggtttg agttcggacg
     3541 aatcgaagtc ggccgcctgc actccgttga tcgcggtgga gatgcggttg cccttgagag
     3601 tgatttcgag tgtattccac tcgccggact tcggtggatt gccggcggcc ttggcaaacg
     3661 tgtagatcga gccggtgccg cgaccgctgg tcgattggtc catgacctga atctcgaaac
     3721 cgtgatggac cgcgtaccat gcgtccttgg gcttgtcggc gatgcgaaga tacacgccag
     3781 aattggagcg gacattggcc gttttgtaga cgacccgaat gacgcaattc ccgagctttt
     3841 cgcgcgcata ccacaagagc cccatgccgt cctcggtacg gagcacaccg tcttcgacga
     3901 caaacttacc tggccccacg tgttcccacc cctcgagggt gcggccgtcg aagaggggtc
     3961 gccaatcgga tgaagaagag gctgattggg ccggccgctc ggcctgagac agcgcgattg
     4021 ttgcgaataa gatcacaagc gacgcattgg ctgcatgacg gaggatggac attgtgaatt
     4081 cctggcggtg gtaatcgttt attcccaggc ccgaaattaa cgcgccggcg gccgccgatc
     4141 aacccgacca cttcactcag gctgaatctg cccgtccttc atcggaattc gccggtcggc
     4201 ggcgcgggcg acgtcgtggc tgtgcgtgac gatcacgagc gtgagccggc gctcatcgcg
     4261 caggcgatgc agcaagtgga taatctcatc ctggctcttg ctatcgagat taccggtcgg
     4321 ctcgtcggcg aggagaagtt gagggtcatt ggcgagagct cgcgcgatcg cgatgcgctg
     4381 gcgctggccg acggagagct ggccggggcg gtggtcgcgc ctgtcgacaa gaccgacttc
     4441 ctcgatcaac cgcaaggcgc gctcgccgcg ctgagacggc ggccaagggc cctcaaacat
     4501 ggggatctga atattttcaa gcgcggtaag ggtcggcatg agataaaagg attgaaatac
     4561 gaatcccacc cggtgagcac gaaaggcgtc g
//
LOCUS       DATAQL010000830         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3903610, whole genome shotgun sequence.
ACCESSION   DATAQL010000830 DATAQL010000000
VERSION     DATAQL010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3903610"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VHS97_14425"
     CDS             <1..216
                     /locus_tag="VHS97_14425"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEX3449451.1"
                     /translation="PRLLIGIGRETEARKVTVRWPSGRIAVAEHLAADQSYLVVEEAH
                     TTDQDARHVFRIIGGERRSVPADRSAR"
     gene            454..951
                     /locus_tag="VHS97_14430"
     CDS             454..951
                     /locus_tag="VHS97_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3449452.1"
                     /translation="MGAIRAGKLLLVEDEHVLRGLIAQFLRGEGYEIVEAADGSEGVE
                     FFATRGPFDLVLLDLNLPILSGVDVCRSIKSRQPAQPVIICSAAILDSHISALLAMNV
                     EQFLSKPYHPLDLLNLITAELSRGNGPQPTELPSTSRTRVWRGGPSHSDATSPHTLVK
                     SPILD"
     gene            1064..1594
                     /locus_tag="VHS97_14435"
     CDS             1064..1594
                     /locus_tag="VHS97_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449453.1"
                     /translation="MAGKPLNRLDLRRQREAAEPLDPMEDETDEVVEELDEEDVVEKK
                     PKKKPKAAPKSKAKSSRSAKPAARMRIVWVVLNDAFKPVGTFDYSQKEAAEARAAELT
                     AKGKGTHFVQRTKEAMPEDAPGIGASLPRVATPAPKASRSKAAAVVEAPVEEEEEIEE
                     DDEAEVDVDEADDDDE"
     gene            complement(1737..1810)
                     /locus_tag="VHS97_14440"
     tRNA            complement(1737..1810)
                     /locus_tag="VHS97_14440"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1774..1776),aa:Ile,seq:gat)
     gene            complement(2075..3109)
                     /locus_tag="VHS97_14445"
     CDS             complement(2075..3109)
                     /locus_tag="VHS97_14445"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX3449454.1"
                     /translation="MQHLRTLEYGSNGTAHEHESRSPSLRLPLARLLQLLMILQSERF
                     PNARRLAEACAVSRRTIYRDLAILEAAGIAVLYHPERQGYQLDRDHWLQPTRLEDNEA
                     LSLLIMSRIGCTQIPVGLLRHARSGLVKVVQGLPGELRSRITNCSELIADDAQALDLG
                     PERQAIHETILSALSQRRRLSLWYRTDGLSPVVATKLSLFRLAWIRGQWCLVGHSSAD
                     REIRVFQIPCIERLELTDEPYLIPPRFCLERFLAKPSLRGDAQTHDIQLRFAPSVALM
                     LRDAPGPPGRKVLPGPNGTIELFFKVELLDDVVLWIIGFGDQVEVIEPDQLRLAVRAQ
                     AERIAHIHSR"
     gene            3331..3403
                     /locus_tag="VHS97_14450"
     tRNA            3331..3403
                     /locus_tag="VHS97_14450"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3364..3366,aa:Phe,seq:gaa)
     gene            3503..>3707
                     /locus_tag="VHS97_14455"
     CDS             3503..>3707
                     /locus_tag="VHS97_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449455.1"
                     /translation="MLPPVEERHQRRNEKCRALTSAKRWAFKRMAMKPTDVLGLSDLK
                     CVTEAADALVAAGLAEPVGDCWRC"
BASE COUNT          808 a         1042 c         1176 g          681 t
ORIGIN      
        1 ccccgactct tgatcgggat aggtcgggaa accgaggctc gcaaggtgac cgtccgttgg
       61 ccctcagggc ggattgccgt cgccgaacac ctggcggccg accagagcta cctggtggtc
      121 gaagaagcgc acacaaccga ccaagatgct cggcatgtct ttcggatcat tggaggcgaa
      181 cgtcgatccg tgccagccga ccgttctgcc aggtgatgga gttggatctt ggaacgacgg
      241 tcggccgacc cgagcgcgat cagcgctcag gctaactatc ctcgaattcg agtggacgtg
      301 accggctgct cgcggtagta tccccggcca gcgcgtgttg ttcaaaaggg accacgctac
      361 cgatcccgac ccggctgcgc cagccgatcg ccaaggggaa ttcgggtgga gggggacaag
      421 gacggaccct gtttcgatca tcaggaggag gccatgggtg cgatacgagc agggaagctc
      481 ttactggtcg aggacgagca tgtgctccgc ggcctgatcg cccagttctt gcgaggcgaa
      541 ggctacgaga tcgtcgaagc ggccgacgga tccgagggcg tcgagttttt tgcaacccgg
      601 gggccatttg acctggtctt actcgacttg aacttaccca ttctgagcgg ggtcgacgtt
      661 tgccgctcga tcaagagccg gcagcccgcg cagccggtca tcatttgcag cgccgcgatt
      721 ctcgacagcc acatcagtgc tttgctggcg atgaatgtgg agcaattcct gagcaagccc
      781 tatcacccgc tggatctcct gaacttgatc accgccgagc tgtcgcgcgg taacggtccg
      841 caaccgaccg agctgccgtc gacctcgcgg acgcgtgtct ggagaggcgg tccatctcat
      901 tccgacgcga cctcgcctca taccctggtc aaatcgccga tcttggatta ggataacggc
      961 ttgtcgtgtc acatgctctc tcacctcccc tttggaaggc gctgggatag cgacactggg
     1021 atcggacgat taagtaccgc catagcacgc gaaggacggt ttcatggctg ggaaaccgct
     1081 gaatcgattg gacctgcggc gtcagaggga agccgccgaa ccgcttgacc cgatggaaga
     1141 tgaaaccgac gaagtcgtcg aagaattgga cgaagaggac gttgtcgaaa agaagccgaa
     1201 aaagaagccg aaggcagcgc ccaagtcgaa ggccaaatcc tctcgatccg ccaagccggc
     1261 cgcacgaatg cgtattgtgt gggtcgtgct gaacgatgcg ttcaagcctg tcggcacatt
     1321 cgactactct cagaaggaag cagccgaagc cagagccgcg gaattgaccg ccaaaggtaa
     1381 agggactcac ttcgtgcagc gcaccaagga ggcgatgccc gaggacgccc ccggaatcgg
     1441 ggcctcgctg ccgcgagtcg ccacgcctgc tccgaaagcc tcacgcagta aggcagcggc
     1501 ggtcgtggaa gctccggtcg aggaggaaga agagatcgaa gaagacgacg aagccgaagt
     1561 cgatgttgat gaagccgatg atgacgatga atgattatcg agggtcattc accgtacggg
     1621 aaggcccgct cgtcgcctag gcctatgact cacaattcgc gatcagcctg atcggtgtct
     1681 atggagacgg ggtgcgaagc tgcaccgtcg tcacttcggc gggccagtaa agtgaatggg
     1741 cgcacgagga ttcgaacctc ggacctcacc cttatcaggg gtgcgctcta gccgtctgag
     1801 ctatgcgccc ttattcacaa ttatcggcat aacctcttgt gagagaggtc tttactgcaa
     1861 tgccatcagc gggcaaaaag gcgactaact ctcacagaaa accatggtaa tcgaggcaca
     1921 caccggatgc aagcggtata gaatgtccac cggcaggatt aggtagacag gagcgcagca
     1981 aaccggccgg gacttcgagg aattgctcgg agccgcagca accgaggaat tgtgcacctc
     2041 gaaacatgaa gcgcattctc cgagtccgcg accctcatcg cgagtggatg tgggcgatcc
     2101 gctccgcctg ggccctgact gcaagtctca gttggtcggg ctcgatcact tcgacctggt
     2161 cgccaaatcc gataatccac aggacgacat cgtcgaggag ttcgaccttg aaaaaaagtt
     2221 cgatcgttcc gttggggcca ggtagaactt tccgaccagg gggacctgga gcgtctctca
     2281 gcatgagggc aacgctcggg gcgaaccgaa gctgaatgtc gtgagtttgc gcgtcgccac
     2341 ggaggctggg tttggcaagg aagcgctcga ggcagaatcg cggaggaatc aagtagggct
     2401 catcggtcag ctcgagccgc tcgatgcaag ggatttggaa aacccggatt tcacgatcgg
     2461 ccgaggaatg tccgaccaga caccattggc cgcgaatcca ggccaagcgg aacaggctaa
     2521 gcttcgtcgc cacgacaggg ctgagtccgt ccgtacgata ccatagagag agccgacgcc
     2581 gctgtgagag ggcgcttaaa attgtctcgt ggatcgcctg acgctcggga ccgaggtcga
     2641 gtgcctgcgc gtcgtccgca atcagctcgc tgcagttcgt gatccggctg cgtagttcgc
     2701 cgggaagccc ctgcacgacc ttgaccaagc cactgcgggc atgccgcaag agtccgacgg
     2761 ggatctgagt gcatccgatg cggctcatga tcaggaggga caaagcctcg ttatcttcca
     2821 atcgagtcgg ctggagccag tggtcccgat cgagctgata gccttgacgt tcgggatggt
     2881 acaggacggc gatgccggcg gcctcgagga tcgccaaatc gcgatagatc gtgcgccggc
     2941 tgacagcgca cgcctcggcg aggcggcgcg cgttgggaaa gcgctccgac tggaggatca
     3001 tcaagagctg cagcagccgg gcaagcggca ggcggagaga tggggaacga gactcgtgct
     3061 cgtgcgccgt gccatttgag ccgtactcaa gagtgcgcaa atgctgcatg tcccagctcg
     3121 tcagcaggtt ctcatcacag gtcggtcctc tggtgaggta gtcatttctt ttgaacctag
     3181 aacatggcga cagcagttga gaactcggcc ggtcgtttcg tgacaacaga caccaaccca
     3241 agccttgaga ttgcggggcc gcgcccgacg tggcaacccg ttgacaggca gagccgagtg
     3301 agttactgtg acatacgtac ataattgagc gggcagatag ctcagttggt agagcacagg
     3361 actgaaaatc ctggtgtcgt gagttcaatt ctcactctgc ccattggtct aaatagcgct
     3421 gaattctaga cttaggttgc ctgccgagat tggcggcgcg cgtgctcgaa ggattggttg
     3481 tagaagggtg gtaggaccat gcttgctacc gcccgtcgag gagcggcatc aacgccgcaa
     3541 cgagaagtgt cgcgccctga ccagtgccaa gcggtgggcg tttaagcgga tggccatgaa
     3601 gccgaccgac gtgctcgggc tgtccgacct gaaatgtgtc accgaggcgg ctgacgcctt
     3661 ggtcgctgcc ggactggccg agccagtcgg cgactgctgg cgctgtg
//
LOCUS       DATAQL010000831         7874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4512873, whole genome shotgun sequence.
ACCESSION   DATAQL010000831 DATAQL010000000
VERSION     DATAQL010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4512873"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VHS97_14460"
     CDS             complement(<1..1151)
                     /locus_tag="VHS97_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3449456.1"
                     /translation="MRRGTLTIGARYVYPVEGPPIEDGCLTIEQGRIAWVGTSRERPG
                     DLDLGNVAIVPGFVNAHTHLELAPLSSKKEKSRDQEDEVSWLRHVVEQRRSGTEQALV
                     AVAAKNVKASIDAGTTFLADITTAGLSWGPVAEAPTRAVVFAELIGLQRDRGLHVSDM
                     AWRWLGSIRPENQVAACARPGLSPHAPYSTAGWLYHKAVASRMPLSTHLAEMPEELRL
                     LRDRDGPLRGFLEELGAWDDDWEPIGPRPADYVRKGELRNADWLIAHGTYLEPDDFWQ
                     LRPEAAPEGHRVAVAFCPRTHARFGHAPHPYRALLERGAIVCLGTDSMASSESLSILD
                     ELRFLHRRDESLSGELLLTMATLFGAWALRAETTTGSIKPGKSADLAIIT"
     gene            complement(1242..3428)
                     /locus_tag="VHS97_14465"
     CDS             complement(1242..3428)
                     /locus_tag="VHS97_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HEX3449457.1"
                     /translation="MRWSNLYVIFRREVRDQIRDRRTLFMIFVLPILLYPILGIGVTK
                     FAMALEQKPRVVIVVGTEYLPATPPLLNPAGDGFNPALFDIPAESDRLVVRPEPASSP
                     WGDPRMRQEAIREGDAAAVMVIPPDLPEQLQRENENEIHIPIHYNSVDEPSKITYLRL
                     KELVDRWRKSIVDRRLERDQKTQSYTEPIQVRALDVATERETGSVLWSRLFPFLLVMM
                     SLTGAFYPAIDLCAGEKERGTMETLLISPASRSEIVLGKFLTVMLASVTTAVLNLVSM
                     GLTGVQLASQVGSLSADASRRSAVTSSVIAPPTLQAAFWMILLLIPLAALFSAVCVSL
                     AVLARSMKEGQYYMTPLYLVCIPLVFVTLMPGVELDLFYSLVPVTGVALLLRALILGN
                     YDAALRFFLPVLLPTVVYAAVALRWAVDQFQREDVLFRESEQFSLAAWLRHILRDREP
                     VPTGGQSALCFVLMLSLTWFLMLYLARQGTDLAIGSVIAGQLIILLPPVMMALVLTSD
                     PQRTLRLAWPQKKYLVLAVALVVALNPLVNELRPIVEMLFPISSVLKTTLTRIMSQAP
                     GLLTSVLVFALIPAICEEFAFRGFILSGLEQQHRTRSAILLSALMFGFLHVLLSLFQQ
                     LFNATLLGIVLGLLAVRSRSIVPGIVFHFLNNAFAVMLGSVVHAPWAMRIVPWIYRNP
                     AEGLYHKQWIVASVLLSGFLLFSLWKWKFDQPGMIARVVRPVEPAC"
     gene            complement(3521..4270)
                     /locus_tag="VHS97_14470"
     CDS             complement(3521..4270)
                     /locus_tag="VHS97_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3449458.1"
                     /translation="MIEVQGLCKRFLDYQRGWKSAITDISFTCEPGAIYGLLGPNGAG
                     KTTTLRILSTVLKPSGGRAIVAGHDVLIEPQAVRANIGYMSASTGIYDRMTAWELVQY
                     FGQLYGVPAVPLRARMEDIFNWLKMNDFRDVLGSKMSTGMKQKVSIARTIVHDPPVLI
                     FDEPTSGLDVLVARAVLQNILELRDRGKTIVFSTHSMHEVEKLCSRLAIIHKGRVQAE
                     GTPSALLDQFDQPDLEELFFYLVERADSAAH"
     gene            complement(4298..5407)
                     /gene="mqnC"
                     /locus_tag="VHS97_14475"
     CDS             complement(4298..5407)
                     /gene="mqnC"
                     /locus_tag="VHS97_14475"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEX3449459.1"
                     /translation="MSSTVAAILQSVIEGERLSVDQGVELYRSATLLELGQAADLVCR
                     RLHPEPYRTFNIDRNINYTNVCTAVCDFCAFYRKVDDAEAYVLDRDVLLEKIRETVAL
                     GGDQILMQGGLHPHLKMEWYEELLHDIKDHFPQVNIHGFSPPEIYHFTKVAKLPLREV
                     LLRLKAAGLGSLPGGGAEILVDRVRGEITRGKVMTDDWLNVNRVWHELGGRSTATMML
                     GHIETIEERVEHFDRVRRLQDETGGFTAFISWTFQPDHTDMAHIPPSGAFEYLRNQAL
                     SRLYLDNVPNIQSSWVTQGPKIGQVALYFGANDMGSLMIEENVVSSAGTVYHLSLAEI
                     KRAIREAGYVPRQRNVFYEYIDQEPAEGCVQAIAG"
     gene            complement(5367..6194)
                     /locus_tag="VHS97_14480"
     CDS             complement(5367..6194)
                     /locus_tag="VHS97_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEX3449460.1"
                     /translation="MAPPIRVGAVSYLNAKPLYYGLCEHFPQIRLSMEVPSRLAEQLS
                     DGELDVGLIPSVEYLRGAVRGYEIIPGFAIAAHGPVRSVKLFSRVPWHRVERLALDAG
                     SRTSQVLARIWLEARHGVRPKQVEELPLGVSALESSADAVLVIGDRAMRVPGESFHDV
                     VDLGEAWHTLTGLPFVFALWVARPGVDLGDLPDALERSRSQGLLHADSLARLHGPRLG
                     LDFATCYDYLTRILSYDLGEPEIAGLSRFAAMAASLGLAPQGVNLVFHRRRDLAKRH"
     gene            complement(6566..>7874)
                     /locus_tag="VHS97_14485"
     CDS             complement(6566..>7874)
                     /locus_tag="VHS97_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449461.1"
                     /translation="PRIASVLLGCGSPPFVTFLSLASYADIDEVVRAQETFGRLELIG
                     IDTYESPLRVVATCLLGTAGFVVGAIWLSRAAVIRFDRAVGRPERARKPREEFVALAS
                     LRNRREFHRARQHRTGSRPLDRDAVTTGSGVSVICTTGGAEELPFRARSWRRKGMVVL
                     CVSALMVPAIAYVIPSWREHRLLHEALAETDRLYPGWHIEDLEAARRQVPDATNGALR
                     VLDAGRALPARWHCAGESPSDAEKNRRNAVAMLAPTLCLAPQTLQSLQTDLNEVGSAL
                     AEARALMDFSEGRFPVAWASDGVSTQLRHPATARDVVNLLAYDVLLRSQKQETDEALL
                     TCHALLNVGRSLGDEPTAVSQRLRIDIRMTTCQQVEFALAHGRASEAGLARLQDALSS
                     PNSFGRFPRTRLPASPCTCGASQTESSSTLWPRMAATMAATPS"
BASE COUNT         1671 a         2451 c         2346 g         1406 t
ORIGIN      
        1 gtgatgattg cgaggtcggc cgacttgccg ggtttgatgc tgccggtcgt ggtttcggca
       61 cgcagcgccc aggcgccgaa aagcgtggcc atcgtcagca gcaattcgcc cgaaagcgac
      121 tcatcgcggc ggtgcaggaa acgaagctcg tccagaatgc tcaggctctc gctcgaggcc
      181 atgctgtcgg tgccgagaca cacgattgcg ccacgttcca aaagcgcgcg gtaagggtga
      241 ggggcgtgcc cgaaccgagc atgcgtccgt ggacagaagg cgacggccac gcggtgtccc
      301 tcgggggcgg cctcggggcg cagctgccag aagtcatcgg gctcgagata tgtcccgtgc
      361 gcgatcagcc agtcagcgtt gcgcaactct cctttgcgca cgtaatccgc ggggcgagga
      421 ccgatcggct cccagtcgtc gtcccaggca cccagttctt ccaggaagcc gcgcagcgga
      481 ccgtcgcggt ctctcagcaa gcgcagctct tccggcattt cggccaggtg tgtcgagagc
      541 ggcatccggc tcgcaaccgc tttgtgatac aaccagccgg cggtgctgta aggtgcatgg
      601 ggtgagagac cggggcgtgc gcaggcggcg acctggtttt cgggacggat cgaaccgagc
      661 cacctccagg ccatgtcgct gacgtgcaac ccgcggtcac gttgcagacc gatcaattcg
      721 gcgaagacga cggcgcgcgt cggtgcctcc gccaccggcc cccacgagag gccggccgtc
      781 gtgatgtcag ccagaaaggt cgtacccgcg tcgatcgatg ccttgacgtt tttggccgcg
      841 accgcgacca gcgcttgctc ggtacccgaa cgcctctgct cgaccacgtg ccgcagccag
      901 gagacttcgt cctcctggtc gcgactcttc tctttcttgc tcgacagcgg cgcgagctcc
      961 aggtgcgtat gcgcgttgac aaagccggga acaatcgcca cgttccccag atcgagatca
     1021 ccagggcgct ctcgggatgt ccccacccag gcgatccgtc cctgctcgat cgtcagacaa
     1081 ccatcctcga tcggcggtcc ctccacggga tagacatacc gcgctccaat cgtgagcgta
     1141 ccgcgcctca tccggcgtct ccgggggcaa acgcctgagg aatggagcgg agccagctca
     1201 tccctggaga tttcctcggt cggtagcggc ctggccgcga atcaacatgc aggttcgaca
     1261 ggcctgacaa cacgcgcgat catgccgggc tgatcgaatt tccatttcca taatgagaaa
     1321 agcaagaatc ccgagagcag aacgctcgcg acaatccatt gcttatgata aagcccttcg
     1381 gcggggttgc gatagatcca agggacaatg cgcatcgccc acggagcatg aaccaccgag
     1441 cccagcatga cggcgaaggc gttgttcaag aaatgaaaga caatccccgg aacaatgctt
     1501 cggcttctca cggccaggag ccccagcaca atccccagga gcgtcgcatt gaaaagttgt
     1561 tgaaagagac tcaagagcac gtgcagaaat ccgaacatca atgccgatag caagatggcc
     1621 gagcgagtgc gatgttgctg ctcgagccct gacaggataa atccgcgaaa ggcaaattcc
     1681 tcgcagatcg ccggaatcag ggcgaagacc aaaaccgagg tcaagagccc cggcgcctgc
     1741 gacatgattc gcgtcagcgt ggtcttcaac accgacgaaa tgggaaagag catctcaaca
     1801 atcggccgca gttcgttgac gagggggttg agggcaacaa ccagcgcgac cgccaaaacg
     1861 aggtacttct tctgcggcca cgccaaccgc aacgtccgtt gcggatccga cgtcaacaca
     1921 agcgccatca tcacgggagg aagtaagatg atcaactggc cggcgatcac gctgccaatg
     1981 gcgagatccg tcccctgccg cgccaggtag agcatcagaa accaggtcaa gctcagcatg
     2041 agcacgaagc ataatgccga ctggccgccg gtcggcacgg gctcgcggtc gcgcaggatg
     2101 tgcctcagcc aggccgcaag gctgaactgc tcggactcgc ggaacaagac gtcctcgcgc
     2161 tgaaattggt caacggccca gcgcaacgcc accgcagcat agacgactgt cggcaacagt
     2221 accggcaaga aaaaccgcag tgcggcgtca tagttgccca ggatcagggc tcgtaagagc
     2281 agggcgaccc ccgtgaccgg gaccaagctg taaaagagat cgagctcgac gcccggcatc
     2341 agcgtgacaa ataccaaggg aatgcaaacg agataaagtg gcgtcatgta atactgaccc
     2401 tctttcatgc tgcgcgcgag caccgccagt gacacgcaga cagcgctgaa gagagcggcc
     2461 aggggaatca agagcaggat catccagaag gcggcctgca aggtcggcgg tgcgatcacc
     2521 gacgacgtga ccgctgaccg gcggctcgca tccgccgaca aagacccgac ctggctcgcg
     2581 agctgcaccc ccgtcagccc catactcaca aggttcaaga cggcggtcgt cacgctcgcc
     2641 agcatcaccg tcagaaattt cccgaggacg atctcggacc ggctcgccgg actgatcaac
     2701 agagtttcca tcgtcccgcg ctccttctcg ccggcacaca gatcgatggc cgggtaaaac
     2761 gccccggtca gcgacatcat gaccagcaag aagggaaaca gccggctcca gaggacactt
     2821 cccgtctcgc gctccgtcgc gacatcgagc gccctcacct gaatcggctc ggtatagctt
     2881 tgcgtcttct ggtcgcgctc aagccggcgg tcgacgatgc tcttacgcca tcggtccacc
     2941 agttccttga gccggagata cgtgatcttg ctcggctcgt ccacgctgtt gtaatggatc
     3001 gggatgtgga tctcgttctc gttctcgcgc tgcaattgct cgggcaagtc aggggggatt
     3061 accatcaccg ccgccgcgtc cccttcccga atcgcctcct ggcgcatccg cggatcgccc
     3121 cagggactcg atgcgggttc tggacgtacg accagccgat ctgattccgc gggaatatcg
     3181 aaaagggctg gattgaagcc gtcgccagcc ggattgagca gcgggggggt cgcgggaagg
     3241 tattccgtcc ccacgacaat cacgacacgc ggcttttgct cgagtgccat ggcaaacttg
     3301 gtcacgccga tccccaggat cggatagagc aagatcggca gcacgaagat catgaagagc
     3361 gtgcgccggt cgcggatctg gtcgcgaacc tcgcggcgga agatgacata gaggttcgac
     3421 caacgcatga aggagctcgc catggatctc gctattcgag cgcgatggaa ggatcttgtg
     3481 ccccgatgag cttgcccgcg gctgaaacca cggtgccggc ttagtgggcg gctgaatccg
     3541 cgcgctcgac cagatagaag aatagctctt ccagatccgg ctggtcaaac tgatcaagta
     3601 acgcgctcgg ggtgccttcg gcctgaacgc gccctttgtg gatgatcgcc agccgcgaac
     3661 agagcttttc gacctcgtgc atggaatgag tggaaaagac gatcgtcttg ccgcgatccc
     3721 ggagctccaa tatattctga agcaccgcgc gggcgaccaa aacatccagc cccgaagtgg
     3781 gctcatcaaa gatcaagacg gggggatcgt gcacgatcgt ccgggcgatg gaaaccttct
     3841 gtttcatgcc ggtcgacatc ttcgagccca gcacatcgcg aaagtcattc atcttcagcc
     3901 agttgaatat gtcttccatc cgcgcgcgta atggcaccgc gggaacgccg tagagctgcc
     3961 cgaaatactg gacaagctcc caggccgtca tccggtcata aattcccgta ctggccgaca
     4021 tgtaaccgat gttcgcccgc accgcttggg gttcgatcaa aacatcgtgg cccgcgacga
     4081 tcgcccgccc cccggacggt ttcaggactg tgcttagaat tcgtagcgtc gtggtcttgc
     4141 cagcgccatt gggccctagc agtccgtaga tcgcacccgg ctcacacgta aacgaaatgt
     4201 ccgtgatcgc gcttttccat ccccgctggt agtcgagaaa gcgtttgcaa agtccctgga
     4261 cctcgatcac gtatcacttc tccgcgagca tcgtggctta gcccgcaatg gcctggacgc
     4321 agccttcggc aggttcttgg tcaatatatt cataaaatac gttacgttgt cgcggaacat
     4381 agccagcctc gcgaatcgcg cgcttgattt ccgcgagcga gaggtgataa acggtaccgg
     4441 ccgatgatac gacattttcc tcgatcatga ggctgcccat gtcgttggcc ccgaagtaaa
     4501 gcgcgacctg accgatcttg ggcccctgag tcacccacga cgactggatg ttgggcacgt
     4561 tatcgaggta aagccgggac agggcctgat tcctcaggta ttcgaacgcg ccggaggggg
     4621 gaatgtgtgc catgtcggtg tggtcgggct ggaacgtcca ggagataaag gccgtgaatc
     4681 cgccggtttc gtcctggaga cgcctgacgc ggtcgaaatg ctcgacgcgt tcttcgattg
     4741 tctcaatgtg gccaagcatc atcgtcgctg tcgaacggcc ccctagctca tgccagacgc
     4801 ggttgacatt gagccagtca tcggtcatga ccttgccgcg agtaatctca ccccgaaccc
     4861 gatcgaccag gatctcggca ccccctcccg gaagactgcc cagtccagcg gccttgagcc
     4921 tcaagagcac ttcccgcaac ggcagctttg ccaccttggt gaagtgatag atctcgggcg
     4981 ggctgaatcc gtgaatgttg acctggggaa agtgatcctt gatgtcgtgc agcaattcct
     5041 cataccattc cattttcagg tgaggatgca gccccccttg catcaaaatc tggtcgcctc
     5101 ctagagcgac cgtctcgcgg atcttctcga gcagcacgtc gcggtcaagc acataagcct
     5161 cggcatcatc gaccttgcga tagaacgcgc aaaagtcgca tacggccgtg cagacgttgg
     5221 tgtagttgat gttgcgatcg atattgaagg tgcgatacgg ctcgggatgg agccggcggc
     5281 acactaaatc ggcggcctgc cccagctcga gaagcgtcgc cgaccgatag agctctaccc
     5341 cttggtcgac gctcaggcgc tcaccttcaa tgacgctttg caagatcgcg gcgacggtgg
     5401 aagacaagat tcactccttg aggcgccagg ccgagtgacg cggccatcgc ggcgaaccga
     5461 ctcaagcccg cgatctcggg ctcgccgagg tcgtaggata agatgcgcgt aaggtaatcg
     5521 tagcacgttg cgaaatcgag tcctagcctg ggaccatgca atcgggccaa cgaatccgcg
     5581 tgcaaaagcc cttgcgagcg gcttcgttcc agggcgtcgg gcagatcacc gagatcgacg
     5641 ccaggccgcg caacccagag ggcaaaaaca aacggcaagc cggtcaaagt gtgccaagct
     5701 tcgccaaggt ccaccacgtc atgaaacgac tcgccgggaa cgcgcatcgc ccggtcgccg
     5761 atgaccagca cggcatccgc cgagctttcg agcgcggaaa cgcccagcgg caattcctcg
     5821 acctgcttgg gtcgtactcc gtggcgtgcc tcgagccaga ttcgcgccag cacctggctg
     5881 gttcgcgaac cggcatcgag cgccagccgc tcgactcggt gccacggcac cctgctgaac
     5941 agcttcacac tgcggaccgg cccgtgagcc gcgatcgcaa agcccgggat gatctcataa
     6001 ccccggacag caccacgcaa atactcgacc gacgggatca gaccaacgtc cagctcgccg
     6061 tccgagagtt gctcggcaag ccggctcggg acttccatcg ataagcgaat ctggggaaag
     6121 tgttcgcaaa gcccataata gaggggcttt gcgtttaagt aacttacggc tccgacacgg
     6181 attggcgggg ccatgacctt cagccctcct ggttgatagc cgaatcctct cccgaatcct
     6241 cataccgact cacagggtga ttcgcaagtc ctgacgggac tttcagaagc ttttgaaatt
     6301 ttaatcgcgt acggtcgaat ggcaaggcca ctcttggata tcaagggggg acgttttcca
     6361 acgattttcc tgtacctagc cgctgcggcc ttccccatct taacgacggg tttggtgttc
     6421 acatggcgct ttttcggcct ccaggaacct ctgaaacctg gtgtcccgga aactagacct
     6481 gatcagggat tggccgccgg ccggtggcgg cgcgcagggt cccacaggcg gaaactcacg
     6541 tcgcgacccg tagccagcaa cgaagtcaac tcggcgtcgc cgccatcgtc gcggccatcc
     6601 tcggccacag agtagatgac gattcggtct gcgaagcgcc gcaagtgcag gggctcgctg
     6661 gcaaacgggt ccgcgggaac cttccgaagg aattcgggga tgagagcgcg tcttggagtc
     6721 tcgcgagccc ggcctctgaa gctcggccgt gtgccagggc gaattccacc tgctgacagg
     6781 tcgtcatgcg tatgtcgatc cgcaaccgct gtgacacggc cgtgggctcg tcgccgagtg
     6841 accggcccac attcaacagg gcatggcagg tcaacaaggc ctcgtctgtt tcctgcttct
     6901 gggaccgcag taagacatca taggccagca ggttgacgac atcgcgcgcc gtcgccggat
     6961 ggcgaagttg ggtagaaacg ccgtccgagg cccaggcgac cgggaagcgg ccttccgaga
     7021 aatccatcag agcccgggct tcggccaagg cggagcccac ttcattcaga tcggtctgca
     7081 gcgattgaag ggtttggggt gcaaggcaaa gcgtgggcgc gagcattgca accgcattcc
     7141 gacggttttt ttcggcgtcg gaagggctct cgcccgcgca gtgccaacgc gccggcagcg
     7201 cgcgccccgc gtccagaaca cgcagcgcgc cattggtcgc atccggtact tgccgacgtg
     7261 cggcttcgag atcttcgatg tgccagccag ggtagagccg atccgtctcg gcaagcgctt
     7321 catgcaatag ccgatgctca cgccatgaag ggattacata ggcgatagcc ggtaccatga
     7381 gggccgatac gcataagaca accattccct tccgccgcca cgatcgcgcg cgaaatggaa
     7441 gctcctccgc acccccggtc gtgcagatga ccgatactcc actgccggtg gtgacagcat
     7501 ctcgatcgag cggccgggat cccgtcctgt gctggcgagc acggtggaat tccctgcggt
     7561 ttctcaaaga tgccaaggcc acgaattcct cgcgcggctt cctggcacgc tcgggacggc
     7621 caactgctct gtcgaatcgg atgaccgcag ctcggctgag ccagattgcg ccgacgacaa
     7681 agccagcggt cccgagcaga caggtcgcaa ccacccgcaa cgggctttcg taagtatcga
     7741 tcccgatcaa ctccagccgg ccgaacgtct cttgagccct aacgacttcg tcgatgtcag
     7801 cgtaagacgc gagcgatagg aaggtgacga aaggcggtga tccgcacccc aagagtacac
     7861 tcgcgattcg gggc
//
LOCUS       DATAQL010000832         5406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4970822, whole genome shotgun sequence.
ACCESSION   DATAQL010000832 DATAQL010000000
VERSION     DATAQL010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4970822"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VHS97_14490"
     CDS             complement(<1..909)
                     /locus_tag="VHS97_14490"
                     /inference="COORDINATES: protein motif:HMM:NF040096.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449462.1"
                     /translation="MDLAIRFPALGQIHLESRFLFSEPQDPNCRQFLERVFQAQEITQ
                     VTIKSAEGTIEAPRAELGFCPKTYTLKQVVERVTQLLNPRAAWPGGSGRHCNESLNGS
                     NGHRQNRGCETCTEPATRNGDGRPAPGSTLEPAGVTITAVTPVRDRKGEIRYFRYGTV
                     VTHWEIKHELAGRLRLKNPVLYRKKDLCQAIERELIGVLGIDSFTTNALTSTVLVHFD
                     RRQLSPAQLIEILESALASAEHPTSKDKMDLHLPLCTASMPLAAVAQFGTPAFLPAAA
                     LLFAYTSIPTFKNAREVLFEEKRLGVD"
     gene            complement(943..1206)
                     /locus_tag="VHS97_14495"
     CDS             complement(943..1206)
                     /locus_tag="VHS97_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449463.1"
                     /translation="MPVTKSEMLFFAAGAAFGAAAGANYPLLKEKFGPIIAAALAGAS
                     SAVGDSYAEVAKKVAEKVEAVQDAMAEMKHSAAHNGTTETPAV"
     gene            complement(1429..1773)
                     /locus_tag="VHS97_14500"
     CDS             complement(1429..1773)
                     /locus_tag="VHS97_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449464.1"
                     /translation="MSDNVATGTLTGDPLKTVAEALETAVQAAKDGAADARATVDKVL
                     PAANRFLTRFAYTTSYAISYGVVFPTVLLAKSIPTNNAVVHGMVDGARAAADMVEQMK
                     SRPAGSSIIQLP"
     gene            complement(1997..2941)
                     /locus_tag="VHS97_14505"
     CDS             complement(1997..2941)
                     /locus_tag="VHS97_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449465.1"
                     /translation="MKPAQPPGNAPSDHMFGSVPVVENASILTPTSGFLKTGYTHTLN
                     AYQGCAFAGSLCGTFCYAQHNQWITKGRAWRLFGAKRNVRAAYRREYDRIKRPKRGEP
                     KALKIYMSSSTDPYLPQEMRLRLTWSILDEMGARPPDVLVIQSHHTLIERDLDLISEL
                     SQRFELWVSLTVETDMDHVPGFPPHASHPARRLETLNTFRTRGVLTQATVSPLLPLAN
                     PRYFALQLDAACDRVILDHYLIGDGSPNGWRTKRTTFAERLERAGFGSWNELAKLWEI
                     RDLFASVLGPDRVLVGCDGFNSVGRVFGEKSTKPSSCY"
     gene            complement(2956..>5406)
                     /locus_tag="VHS97_14510"
     CDS             complement(2956..>5406)
                     /locus_tag="VHS97_14510"
                     /inference="COORDINATES: protein motif:HMM:NF042902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6797 domain-containing protein"
                     /protein_id="HEX3449466.1"
                     /translation="VCANCHGTEDRPGSLPSAPRFAAAALKNGSDPYRMYRTLTVGFG
                     QMAAQTWMVPRQKYDVIHYIREAYFKPGNSDRYTRVDRSYLDQLPKGTSHGPPPAAIE
                     PWIVMDYGPSLSATLEVSDDGSNIAAKGIAIRLDAGPGGIARGRAWVVYDHDTMRLAA
                     AWTGQGFIDWNGINFNGRHQVHPRIVGKVQFTSGDVPGWSNPENESFADLRERGRDGR
                     PYGPLPRRWARYKGLYRHGDQVILAYTVGTASILEMPGLETDPAHPDHPIFTRTLEIG
                     PAPHDVTMRVAPAATAVSVAGGGQASVFQREGIILLRVPRSESSRRLKVLMANLAAGE
                     LQSYAKSSPAAIALEPLTKGGPTRWPEIVKTEDVRGQDDRPFAVDKLTIPTDNPWLCQ
                     MRPSGFDFMPSGREAAVCTWDGDVWLAGGLATPGGGLTWRRIASGLFQPLGLKIVAGQ
                     IYVACRDQIVRLRDVNGDGETDFYENFNSDHQVTGHFHEFAMGLQTDAEGNFYYAKAA
                     RHGLPALVPHHGTLLRVSKDGTRTDILASGFRAPNGVCVNPDGTFFLSDQEGFWTPKN
                     RINCVKVGGFYGNMWGYHNVTDTSDSAMEPPVCWITNAMDRSPAELVWVDSSQAAWKP
                     LRGALLSLSYGNGKIFVVPHEISGGKMQGGVCALPIPPLPTGIMRGRFNPIDGDLYTC
                     GLFAWAGDRTQPGGFYRVRATGKPIVVPIGLYARHDGLAITFTEPLKREDADLTSHYT
                     AKTWALERTVKYGSDHHDEHSVAITKATLSGDGRTVLLEIPNLRPTWCMEVTYDLQAA
                     SGEPAHGVIHNTIHQLRD"
BASE COUNT         1056 a         1559 c         1670 g         1121 t
ORIGIN      
        1 atcgactccc agccgctttt cctcgaacaa aacttcacgc gcgtttttga aggtcgggat
       61 cgaagtatag gcaaatagga gcgcagcagc cggcaaaaac gccggtgtac cgaactgagc
      121 aacggccgcc agcggcatcg aggccgtgca gagcggcagg tgcaggtcca tcttatcctt
      181 gctcgtagga tgctcggcgc tcgccagtgc ggattcgaga atctcgatca actgcgccgg
      241 cgataattgt cgccggtcga agtgaaccag caccgtgctc gtgagcgcgt tggtcgtgaa
      301 cgaatcaatc cccagcacgc caatcaattc gcgctcgatt gcctgacaca agtctttctt
      361 gcggtacagg accgggtttt tcagccgcag ccttccagcc agctcgtgtt tgatctccca
      421 gtgtgtaacg actgttccat agcggaagta gcggatctcg cccttgcgat cgcgcaccgg
      481 cgtgaccgca gtgatggtca cgccggcggg ttccagggtt gagccaggcg cgggccggcc
      541 atcgccattg cgagtcgccg gctctgtgca cgtctcacac ccgcggtttt ggcgatggcc
      601 gttcgagcca ttcaagcttt cattacagtg acgacccgat ccgcccggcc acgcagcacg
      661 gggattgagc aattgcgtca cgcgctcaac aacttgtttg agcgtatagg tcttcgggca
      721 aaaacccaat tccgcgcgcg gtgcttcgat cgtcccttcc gcgcttttga tggtgacctg
      781 ggtgatttcc tgcgcctgga agacgcgctc gaggaactgg cgacaattgg gatcttgggg
      841 ctctgaaaac agaaatcggc tctccagatg aatttgcccc aaggccggaa aacgaatggc
      901 aagatccatg gatgctcgtg gttatgggct cagagcggcg agttacactg caggggtttc
      961 ggtcgttccg ttatgggcgg ctgagtgttt catctcggcc atggcgtcct ggacggcttc
     1021 cactttttcg gcaacctttt tggctacctc ggcgtacgaa tcgccgacgg ccgaactggc
     1081 accggccagt gcagctgcaa tgatcgggcc aaacttctcc ttgagaagcg gataattcgc
     1141 accggcagca gcaccgaacg cagctccagc cgcgaagaac agcatttccg atttagtcac
     1201 gggcatcgcg gtcagcctcc gtttccgaga tcaaggttca ctccaagttt caaaaccccg
     1261 gcgaattatc ggaataatcc catcaggcgg acgagaccat tttccggata aattccctcg
     1321 catccatcat acgcgttttc cggatgcgcg gttcgccgca agattattca ctgcttttcg
     1381 ctgcgcaggc cgctccggcg attcaacacg gtcattctac gagtgtggtc atgggagctg
     1441 gatgatcgac gatccggccg gccgactctt catctgctcg accatgtcgg ccgctgcacg
     1501 agcaccgtca accattccat gcacgactgc attgttggtc gggatcgatt tggcgaggag
     1561 gaccgtggga aaaaccactc cataggaaat cgcgtagcta gtggtatagg caaatcgcgt
     1621 caaaaagcgg ttggcggccg gaagaacctt atcaaccgtc gctcgggcgt ccgcggcacc
     1681 atcctttgcc gcctgcacgg ctgtctccaa ggcttccgcg acggttttga gaggatcacc
     1741 ggtcaatgtt cctgtggcga cattgtcaga catgaactag gacctcgtgc gatttttggg
     1801 tgacaatcgt gatcggacag aatcgacgaa tgctccggcc gatttgggaa aacgggtgcg
     1861 ccggcggacg tccttttgga aatgacgtcg acgaagctta tgacatcaag ttggccgcga
     1921 cagacaactc tgctgcacaa attgcgccat tctcagacga gggattgata aaagttaata
     1981 taggcggttt gggttgtcaa taacaactcg atggtttggt ggatttttcc ccgaaaacac
     2041 gtccgacgga gttaaagccg tcgcagccga ccaaaacacg atctgggccc aaaacgctgg
     2101 caaacaagtc gcgaatttcc cagagctttg ccagttcgtt ccatgatccg aaccccgccc
     2161 gttctagacg ttccgcaaaa gttgttcgtt tggtgcgcca gccgttgggc gagccgtcgc
     2221 cgatcaggta atggtcgagg atgactcggt cgcacgcggc gtcgagttgg agcgcaaaat
     2281 agcgagggtt ggcgagcggc aaaagcgggc tgactgtcgc ctgggtgagc acgccacggg
     2341 tgcggaacgt gttgagagtc tccaaccggc gggccgggtg actcgcgtgc ggcggaaagc
     2401 cgggcacatg gtccatgtcg gtttcgacag ttaacgaaac ccagagttca aatcgctgag
     2461 atagttccga aatgaggtcg agatcgcgtt cgatcagtgt gtgatggctt tgaatgacga
     2521 gaacgtcggg ggggcgtgca cccatttcat cgaggatcga ccatgttagc cgcaggcgca
     2581 tttcttgggg caaatagggg tcagtgctgc tactcatgta gatcttgagg gccttcggct
     2641 cgccacgttt cggccgcttg atccggtcgt actcgcggcg gtaggccgca cggacgttgc
     2701 gcttggcgcc gaagagtctc caggctcgtc ccttggtaat ccactgatta tgttgagcgt
     2761 agcaaaaggt gccgcagagg gaccctgcga aggcgcaccc ctggtaagcg ttgagggtgt
     2821 gcgtgtagcc ggttttcaaa aaaccactgg tgggtgtcag aatcgacgcg ttctcgacca
     2881 cgggaacaga cccgaacata tggtcggacg gcgcatttcc aggcggctgc gcgggtttca
     2941 ttgcagtgat gccagtcaat cgcggagttg atgaatcgta ttatgaatca cgccatgggc
     3001 gggttcgccg ctcgcggctt gcagatcgta tgtcacttcc atgcaccagg tcggccggag
     3061 attagggatt tcgaggagca cggtccgtcc atcgccggag agtgtcgcct tggtaatcgc
     3121 aaccgagtgc tcgtcatggt ggtccgagcc gtatttgacg gtccgctcta gcgcccaggt
     3181 cttggccgtg taatgcgacg tcagatcggc gtcttcgcgt ttgagaggct ctgtaaaagt
     3241 gatcgccagg ccgtcgtgtc gcgcgtacag cccgatcggt accacgatcg gcttaccggt
     3301 ggcacgcacg cgataaaaac cgcccggctg ggtccggtcc ccggcccagg cgaacagacc
     3361 gcaggtgtag aggtcgccgt cgatcggatt gaaccgaccg cgcatgattc cggttggcag
     3421 cggcgggatc ggcaatgcgc acacaccacc ctgcatcttg ccccctgaga tctcgtgcgg
     3481 aacgacgaag atcttgccat tgccatacga cagcgagagc agagcccccc gcagtggttt
     3541 ccaggccgcc tgcgaactgt ctacccacac aagttcggcg ggcgagcgat ccatggcgtt
     3601 ggtgatccag cagactggcg gctccatggc gctgtcagag gtgtcggtga cattgtggta
     3661 tccccacatg ttgccataga agcccccgac tttgacgcaa ttgatccggt ttttgggcgt
     3721 ccagaaaccc tcctggtcgg agaggaaaaa ggtgccgtcg gggttgacgc aaacaccgtt
     3781 gggagcgcga aaaccggacg ccaggatgtc ggtgcgcgtg ccgtccttgc tcacgcgtaa
     3841 cagcgtaccg tggtgcggaa cgagcgccgg cagaccgtgg cgcgctgctt tggcgtagta
     3901 gaagttgccc tcggcgtccg tttgcaggcc catcgcgaat tcgtggaagt gcccagtgac
     3961 ctgatgatcg ctgttgaaat tctcgtagaa gtccgtctcg ccgtctccgt tcacgtcacg
     4021 cagacgcacg atttgatcgc ggcaggcaac gtatatctga cccgcgacga tcttcaggcc
     4081 caatggctga aacagtcccg atgcgattcg gcgccacgtg aggccgcccc caggcgtggc
     4141 gagaccaccg gccaaccaga cgtcgccgtc ccaggtacat acagctgctt ctcgtcccga
     4201 tggcataaag tcgaaaccgg agggtctcat ctggcagagc catggattgt cggtcgggat
     4261 cgttaactta tcgactgcga acggccgatc gtcctggccc cggacatcct cggttttgac
     4321 gatttccggc cagcgtgttg ggccgccctt tgtgaggggc tccagtgcga ttgcggcggg
     4381 cgatgacttg gcgtaggact gcaattctcc ggcggcgagg ttcgccatca ggaccttcaa
     4441 ccggcgcgag gactcggatc gaggcacgcg caacaggatg ataccctcgc gctggaacac
     4501 gcttgcctgg ccgccacccg ccacgctgac ggctgttgcg gccggcgcga cacgcatcgt
     4561 cacgtcgtgg ggcgctgggc cgatttccag ggtgcgcgtg aagatcggat gatccgggtg
     4621 cgcagggtcg gtctcgaggc ctggcatttc gagaatcgag gctgttccca cggtatacgc
     4681 gaggatcacc tggtctccgt ggcgatagag tcccttataa cgggcccatc gccgcggcaa
     4741 gggaccgtaa ggccgtccgt ctcgacctcg ttcgcgcagg tcggcgaagc tctcgttttc
     4801 ggggttcgac cagcccggga cgtcgccgct cgtgaattgt accttgccga cgatgcgcgg
     4861 atggacctgg tggcggccgt taaagttaat tccgttccag tcgataaatc cctggccggt
     4921 ccaggcggcg gccaggcgca ttgtgtcgtg gtcgtagacg acccaggctc tcccgcgagc
     4981 gatccctccc ggcccggcat cgagccggat ggcgatcccc ttggcggcga tgttcgaccc
     5041 atcatcgctg acttccagcg tggccgacag gctgggaccg tagtccatca cgatccaagg
     5101 ctcgatcgct gcaggcggcg gtccgtggct cgttcccttg gggagctgat ccaagtaact
     5161 ccgatcgacg cgagtgtacc tatcggaatt gccgggcttg aaataggcct cacgaatgta
     5221 gtggatcacg tcgtattttt ggcggggaac catccacgtc tgcgcggcca tctggccgaa
     5281 tccgacagta agagtgcggt acatgcggta gggatcgcta ccgtttttca gggccgcggc
     5341 ggcaaatcga ggcgcggaag gcagcgaacc gggccgatcc tcggttccat ggcagttggc
     5401 gcagac
//
LOCUS       DATAQL010000833         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1611435, whole genome shotgun sequence.
ACCESSION   DATAQL010000833 DATAQL010000000
VERSION     DATAQL010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611435"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VHS97_14515"
     CDS             complement(<1..346)
                     /locus_tag="VHS97_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449467.1"
                     /translation="MTAPATKSKSAPVHFRDRRRISRLARVGLLLALAFGWCAPAVGA
                     DLKEAEKLYRTGRYDECARLVDEEMGVTGWSEPWRHLKIKAELATGKYDEAKASLEGA
                     LRRFPASISLRLL"
     gene            518..1669
                     /locus_tag="VHS97_14520"
     CDS             518..1669
                     /locus_tag="VHS97_14520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX3449468.1"
                     /translation="MSRYVQAFGLGLFWSLQSWAHGAGAAEPPRVTGHSLVYREPGRF
                     AGWPANHGMWSWGNEILVGFSRGFDKDRGPYHHIDKEKPEEFLLARSSDGGATWSVEQ
                     PRPAGILAGTPGMRHGIMPPGVAPDDPTELQEPIDFTHPDFAMTVRMENTNAGISRFF
                     LSYNRGHTWRGPYALPLFGQKGVMGRTDMIVDGPRDCLLFLTASKTNGLEGRPFCART
                     TDGGLNWRFLSFIGPEPTGYAIMPSSVRLSSSDLVSTVRMRDTTKSWIDAYASHDNGQ
                     TWSFLSMPEPDTGEGNPPSLLRLGDGRLCLSYGRRALPYGILARLSGDQGKTWGQPIV
                     LRDDGAAADVGYVRSIIRPDGKVVAVYYYNDRSSPERYLAATVWDPGKP"
     gene            complement(1709..2383)
                     /locus_tag="VHS97_14525"
     CDS             complement(1709..2383)
                     /locus_tag="VHS97_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449469.1"
                     /translation="MQKARAKIAYDPTDRYKVQQILGWTVLVNKSFLESQPELAKQTL
                     TLMREQLRQIVRGVPAEAVKKLRTIRIWVEEKEPHPPCMTYHPNIVWLKEYGKNPDKA
                     RCVEVANARNFLEWIRQQPWMVLHELAHGYHHQFLDRGFENAEIQTAFRGATKAKRYE
                     SVPRNNGKTEKAYAATNAKEYFAEASEAYFGTNDFYPFVRSELKKHDPDMFRLLEKLW
                     HPVAPD"
BASE COUNT          476 a          765 c          762 g          543 t
ORIGIN      
        1 ccaagagacg caaggaaata ctcgccggaa atcgccgcag cgccccctcg agcgaagcct
       61 tggcttcgtc atactttccg gtcgccagct cagccttgat cttcaggtgg cgccagggtt
      121 cgctccatcc tgtaacgccc atctcttcgt cgaccaggcg cgcacactcg tcgtagcgcc
      181 ctgtgcgata caacttctcg gcctctttta gatccgcgcc aactgccggc gcgcaccaac
      241 cgaaggccag cgcaagcaga aggcctactc tcgcaagccg gctgatacga cggcgatctc
      301 ggaaatgaac gggcgcggac tttgatttcg tggctggcgc agtcattcag cccttccatt
      361 ccgagtggcc ttgagatcac gcgcaaagca ccaagaaatt aattatagtg ctacgctcgt
      421 ccgcaatgcc aagactttcc accaacgccc cggggaggta agaatactcg cgggcgagcg
      481 ctttcatgca cgatgtaaga gtggtgtggg gatgctgatg tcgcgttacg tacaagcgtt
      541 cggtttgggt cttttttggt ctttgcaatc gtgggcgcat ggcgctggcg ccgccgagcc
      601 gccacgcgtc acggggcatt ccttggtata ccgtgagccg ggccggttcg cggggtggcc
      661 ggccaaccat ggcatgtggt cgtggggcaa cgagatcctg gtcggcttca gtcgtggatt
      721 tgacaaggac cggggaccgt accaccacat cgacaaggag aagccggagg agtttctgct
      781 ggcgcgtagc tccgacggcg gcgcgacctg gtcggtcgag cagccccgtc ccgcggggat
      841 tttggcaggc acacccggga tgcgccacgg aatcatgcct cctggagtcg cgccagacga
      901 tccgaccgag ctgcaggaac ccatcgattt cacccatccc gactttgcca tgaccgtccg
      961 catggagaac acgaatgccg ggatctcgcg cttctttttg tcgtacaacc gcggccacac
     1021 ctggcgcggc ccgtacgcgc tgccgctgtt cggccagaaa ggggtcatgg gccgcaccga
     1081 catgatcgtc gatggtcccc gcgactgtct cctcttcctg accgcctcga aaactaacgg
     1141 cctggaaggc cgcccgttct gcgcccgaac gaccgacggc gggctcaact ggcgcttcct
     1201 ttcgttcatc ggccccgagc caaccgggta tgcgatcatg ccatcgtcgg tgcgtctctc
     1261 ctcgagcgac ctcgtatcga cggtgcggat gcgcgatacg accaagagct ggatcgatgc
     1321 ttatgcgtca cacgacaacg gccaaacttg gtcgttcctg tccatgccag agcccgatac
     1381 cggcgagggc aacccgccta gcctgcttcg tctcggtgac ggccgactct gcctgagcta
     1441 tggccgccgc gcccttccgt acgggatcct ggcacgtctg agcggcgacc agggcaagac
     1501 ctggggacag ccaatcgtct tgcgcgatga tggtgctgcc gccgacgtcg gctacgtccg
     1561 gtcgatcatc cgccccgacg gcaaggtcgt agcagtctac tattacaacg accggtccag
     1621 cccagagcgc tacctcgccg ccacggtctg ggatccgggg aaaccctgag acgggtcgtt
     1681 atcattcaat gcgatcacga ccaaatacct aatccggcgc aacaggatgc cacagctttt
     1741 cgagcagtcg aaacatatcg gggtcgtgct tcttgagctc cgatcgcacg aacgggtaga
     1801 agtcgttggt gccgaaatag gcctcgcttg cttcggcgaa atattccttg gcattggttg
     1861 cggcataggc cttctctgtc ttgccgttgt tccgtggcac tgattcgtaa cgctttgcct
     1921 tcgtagcgcc gcgaaaggcg gtttgaatct ccgcgttttc gaacccgcga tctagaaatt
     1981 gatgatgata tccgtgcgcc agctcatgga gcaccatcca gggctgttgc cggatccatt
     2041 cgaggaagtt gcgcgcattg gccacttcga cgcaacgagc cttgtcggga ttcttgccgt
     2101 attctttgag ccagacgatg ttcggatgat aagtcatgca gggagggtgc ggctcttttt
     2161 cctcgaccca gattcgaatg gtgcgcagct tcttgacggc ctcggctgga acgccccgga
     2221 cgatctggcg gagctgctct cgcatgaggg tgagcgtttg tttcgcgagt tcgggctgtg
     2281 actccaggaa gctcttgttg acgagcacgg tccagcccag gatctgctgg actttatatc
     2341 gatcggttgg atcgtaggcg atcttggcgc gggccttctg cacgcgaacg tttttcgcag
     2401 gagggcttgg ttttggttgt cttgtcgcca aagcgctatc cgtgacgagt attgtgagag
     2461 cgaatgacaa gcttacaagg ctcggaaatg tccaatgaca aggacgccat gttggcatgg
     2521 tgtgagcttt cagtgaacaa cgagag
//
LOCUS       DATAQL010000834         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4665504, whole genome shotgun sequence.
ACCESSION   DATAQL010000834 DATAQL010000000
VERSION     DATAQL010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4665504"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..1010
                     /locus_tag="VHS97_14530"
     CDS             417..1010
                     /locus_tag="VHS97_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3449470.1"
                     /translation="MSNDDELNDLLSRARAGDEAAISDFLSRFEPEVTMMVRSRLPRK
                     LRTQFDSTDFVQAVWKSLLVESGQNSRDFEDVEHFRGFLYGMVRNKVSEQQRRLTKTA
                     KYDLTREERLYIRRGDREVAREVVSPEPSPSQAVQASDRMAQLTAGKSPREVEILTLR
                     RLGLTLLEIAQRTGINERSVRRVIDSARSLLESRRWH"
     gene            1001..>3297
                     /locus_tag="VHS97_14535"
     CDS             1001..>3297
                     /locus_tag="VHS97_14535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449471.1"
                     /translation="MALGLRIASAVVESAKFVNSSALLHEFKAAREGGRAVAVEDYLY
                     RLDPADSRGAVELIYQDYCLAEARGSKPEVADYLARFPQHEAALERLILLHSACPPSL
                     MARWIETTPSSVPPEAGDEIGPYVLKRELGRGGFARVFLAEQTNLENRLVVLKVSKTM
                     TREPWLLARVRHAHIVEIVSHATVDDGAFHLICMPFWGGATLAALLAQRRQSGRRTCT
                     GIDLLADLDLVAAPEFPTVHPARPAREILAGLTYDQAIAWVGARLAEALDHAYSRDVA
                     HGDVKPSNILLSADGNPMLLDFNLARDWSAAGMDLSKGDTGGTLAYMAPERLQGLAGD
                     LATRDDWDKDKSSVELASVHPSSSPDRTCESSSSDLQECGPHQADIYSLGVVLLEALT
                     GEPPAQRDVSATSAGGTDFDQLRAMAGAYASSRGRGAMLAIRESEQISRRSITPGLRA
                     ILSRCLDPDPARRYRRGLELAEDLDRWRTDRPLAYTSEPFWRQIAPRALRRHRRTLIL
                     TVAIVSLFVGLPTTAVVTLTSGKKLNFAAEHKLSRLWDDPHSPTYGLQRPGSLRPIEP
                     GDRKVVETARLALDDYNVRGPGDWRQRDDVRFLPPADREELELWLLEQGYRYCRALEE
                     RPEIRRDDWMRAREYISRLTASRPLQVFSALDRRLRAKLGVDPLSSPALAFGEVSRAG
                     LDPTPSTAAPGDGRWIDMYLLGVAAEWDVTTESAVQIDTQSLAQSQAAGRALGHYNAM
                     LEIRPDSYWGNYRAAVACFAMEEFD"
BASE COUNT          630 a          990 c         1026 g          651 t
ORIGIN      
        1 ttcgacgatc acgtgggtcg tgatatctgg gtaaaaaatg gagggactgt ggagtaatag
       61 gcttgttttt caggaatttt ctcgccgcag agacgtgaat tgacgatccg ctaaagccgg
      121 tgcgatcgtc ggttttaatt aacctttttt gacaactggc tggaatcttg cgccgcgaaa
      181 aggtttaatt aactggtcgc gagggatgct ggcgacgagt aagtggatgt gcgtctggct
      241 ccgacaccga ttctggcgag ggttgcctgg gatgaagttc ctcgggtgtc ggggcaaaca
      301 ggattatcgg gaatgagggc ctgaattccg cttcggtcta cgtgcgcggt acggatgtag
      361 gcttgaagat atcagggagg gtatcgaagg tcgattgaac attgcggggc gaagtcatgt
      421 ccaacgacga cgaactcaac gacctgctat cgcgggcacg agcgggcgac gaagccgcga
      481 tcagcgactt tctatcgagg ttcgagccgg aagtcacgat gatggtccgt tcgcggctac
      541 ccaggaagct tagaacccaa tttgactcga ccgactttgt tcaagcggtc tggaagagtt
      601 tattggttga atcgggtcag aattctcgcg atttcgagga tgtcgagcat ttccgtggtt
      661 tcctctacgg catggtccgc aacaaggtga gcgaacagca acggcggctt accaagaccg
      721 caaaatacga cctgacgcgc gaagagcggc tctacatccg ccgtggcgac cgcgaggtcg
      781 cgcgggaggt cgtttcgccc gagccgtcac caagccaggc ggtccaggcc agcgatcgaa
      841 tggcgcagtt gacggcgggc aagagccccc gcgaggtcga gatccttacg ctgcgccgac
      901 tggggctgac gcttttggag atcgcccaac gaaccggaat caacgaacgg tccgtacggc
      961 gtgtaatcga ttcggcccga tccctcctgg agtctcgccg atggcactag ggctccgtat
     1021 cgccagcgct gttgtcgagt cggccaagtt cgtgaattcg tcggcactgc tgcacgaatt
     1081 caaagctgcc cgggaggggg gccgcgcggt cgccgtcgaa gattacctct accgtctcga
     1141 ccccgccgat tcccggggtg ccgtcgagct catctaccaa gactattgtc tggcagaggc
     1201 gcggggatcg aagcccgagg tcgcagacta cctggcccga tttccgcagc acgaggcggc
     1261 gcttgaacga ctgatattgc tgcacagcgc ctgtccaccg tcgctcatgg cccgctggat
     1321 agagaccact ccatcgtcgg ttccgcccga agccggcgat gagatcgggc catacgtcct
     1381 caagagagag ctcgggcgcg gcggattcgc tcgcgttttt ctggccgagc aaaccaatct
     1441 cgaaaatcgc ctcgtcgtgc tcaaggtctc aaaaacgatg acccgtgagc cgtggcttct
     1501 ggcgcgggtg agacacgctc atatcgtcga gatcgtctcg catgcaactg ttgacgacgg
     1561 tgcgttccat ttgatctgta tgccgttctg gggcggagca acgttggccg cgcttctggc
     1621 ccagcgcagg cagagcggac gacggacctg cacggggatc gatttactcg ccgatctcga
     1681 tttggtcgct gctcccgaat tcccgaccgt acatccggcg cggccggccc gtgaaatcct
     1741 ggccggcctc acctacgacc aggcgatcgc ctgggttgga gcgcggctcg ccgaggcgct
     1801 tgaccacgcc tacagccgcg acgtggccca cggcgacgtc aagccttcaa acatcctgct
     1861 ctcggccgac ggcaacccga tgctcctcga ctttaacctc gcccgtgact ggtccgcggc
     1921 gggaatggac ctatccaagg gcgacacggg cgggactctt gcctacatgg ctcccgaacg
     1981 cttgcaaggg ctcgcaggcg atctagcgac tcgagacgac tgggataaag ataaatcgtc
     2041 ggtcgagcta gcatcagttc acccatcgag ttcacctgat cgaacttgtg agagttcttc
     2101 ctccgacctg caagaatgcg ggccgcatca ggcggatatc tactcgctgg gcgtggtctt
     2161 gctggaagct ttgaccggcg agccgcctgc acaaagggac gtgtcggcta cgtctgcagg
     2221 tggcactgac tttgaccagt taagagccat ggccggcgcg tacgcgtcga gccgcggccg
     2281 cggcgcgatg ctcgcgatcc gcgaatcgga acaaatcagc cggcgctcga tcacgccggg
     2341 cctgcgcgct atcctctcgc gctgcctcga tccggacccc gcccgtcgtt atcggcgcgg
     2401 gttggaactc gccgaagatc tcgaccgatg gcgaactgac cggccgctgg cctacacttc
     2461 cgagccgttc tggcgccaga ttgcgccgag ggctttgcgc cggcatcgac gaacgctgat
     2521 cttgaccgtg gcgatcgtat cgctttttgt cggattgccc actacggctg tcgtgacgct
     2581 cacttcgggc aaaaaactca acttcgctgc ggagcacaag ttgagtaggc tttgggacga
     2641 cccccattca ccgacatacg ggttgcagcg gccgggatcg ctccggccga tcgagcccgg
     2701 cgatcgcaaa gtcgtcgaga ccgctcgcct cgcattggat gactacaacg tccgcggccc
     2761 tggtgactgg aggcagcggg acgatgtccg cttcctcccc ccggctgacc gcgaagagct
     2821 cgagctctgg cttctggaac agggatatcg gtattgccga gccctggaag aacgacccga
     2881 gattcggcgc gacgactgga tgcgggcgcg cgaatatatc agtcgactta cggcatcgcg
     2941 gccactccag gtcttcagtg cgctggaccg ccgcctccgc gccaagctcg gtgtcgatcc
     3001 gctatcatcg ccggctcttg cctttgggga agtttctcga gccggcctcg atccaacacc
     3061 ctccaccgcc gcgcccggcg acggacgctg gatcgacatg tacttgctgg gagttgccgc
     3121 ggagtgggat gtcacgaccg agtcagccgt ccagatcgac acgcaatcgc tggcccagag
     3181 tcaggcggcc ggacgggcac tcggtcatta taacgcgatg ctggaaatcc gccccgattc
     3241 gtactggggt aactatcgag cggccgtcgc ctgctttgcc atggaggaat ttgacaa
//
LOCUS       DATAQL010000835         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4055510, whole genome shotgun sequence.
ACCESSION   DATAQL010000835 DATAQL010000000
VERSION     DATAQL010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4055510"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VHS97_14540"
     CDS             complement(<1..207)
                     /locus_tag="VHS97_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3449472.1"
                     /translation="MKRGKRVKEAGAAAPKQGRKGAATREAILQSAQIAFTRSGYDGV
                     GVREIAQGAGVTAMMVNRYFGSKEQ"
     gene            355..675
                     /locus_tag="VHS97_14545"
     CDS             355..675
                     /locus_tag="VHS97_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3449473.1"
                     /translation="MDLKLSGKRALVTGSSAGLGEAMVKLLAAEGVAVVVHGRNADRT
                     HRVANAIHTAGGRADVAMGDLATDAGADAVTMAALAGGPIDILVNNAGCYHHMTSMEA
                     TPRL"
     gene            690..1523
                     /locus_tag="VHS97_14550"
     CDS             690..1523
                     /locus_tag="VHS97_14550"
                     /inference="COORDINATES: protein motif:HMM:NF006114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX3449474.1"
                     /translation="MPKVWLITGASRGLGRALADAVVAGGHMLVATARNPAQLADLAL
                     HAPDRIRTFALDVTDAGVAERAIQSAVDAFGRLDVLVNNAGYGNISSIEETSLVDFRE
                     QIETNLFGTINVTKAAIPLMREQRSGHIIQVSSIGGRLTPPGRGAYATAKAGLGAFSE
                     VLAKEVGPLGIKVTIVEPGGFRTDFAGKSTTIHDSRPEYDSTVGAMARYQRDYNGKQP
                     GDPAKAAAVILHIAGLDEPPLRLLLGTDAANAAEKKNLERIEADRRWRDLSVSTDFHL
                     L"
     gene            complement(1796..3388)
                     /locus_tag="VHS97_14555"
     CDS             complement(1796..3388)
                     /locus_tag="VHS97_14555"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3449475.1"
                     /translation="MGETYPISRRRLLRGMGVGLALPWLEIMSPTIAATDSTKKPPLR
                     LGVLFKGNGVHPPSWDISGASETDFPLSPLLEPLAPMRDKVLILSNLAHREGGGSHHA
                     AAVAFLSGANTPGSIDVPQPVTIDQLAADRIGRHTPLRSLELTADSLFLSQPRCSYIS
                     YGVDGKPVRREDDPQIVFDRMFRGYSSGERLSQTRSILDAVQEDARSVRRSASTADRR
                     TLDAYFDAVRGVERTIEAASGKPNGDRWRPRVAPELIRPPVLMDLPERVKALLNLMVL
                     AFWTDSTRIASFMLANDNSRLVFDFLGINEEHHYLSHFVRHPGVEYIHRFNAITRWHV
                     AQFAYLIDRLAKIQEGDGTLLDNTLLLFGSGLKHGDYHSAGDLPVVLAGGGGGTLKTG
                     RWIRYAEPQPYANLLLSLLHRLGIDAPRFGSSTGPLPGLDRPLGPVPGVTDDGTWRVV
                     YDTDRQLTARGLLRASDKAEEINIYYLQLSNGEKIKVQARFGNLDDTRFDEFCGRVVT
                     VGGTYTRGDGETIIRALTSVRP"
     gene            complement(3442..>4145)
                     /locus_tag="VHS97_14560"
     CDS             complement(3442..>4145)
                     /locus_tag="VHS97_14560"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HEX3449476.1"
                     /translation="FLNPTLGYHYRLKGYKRERLIKDGGYDWPDPLRRVALDDPNRGA
                     VLGMAATLVATSAPERTSPIRRGVWILDAILGQRPPDPPPNIPPLEEAGKGRRPRSMK
                     EQMEMHRGEVSCARCHDAIDPLGLALENFGPLGEWRTSDKFGTIDASTTLPGGERVSG
                     PAELKALLATQYREPFVRNASERMLSYALGRAILYTDRPTIDRLVDRLKANDYRFSIL
                     VKGIVASVPFGYRED"
BASE COUNT          769 a         1277 c         1323 g          776 t
ORIGIN      
        1 ctgctccttc gagccgaagt atcggttgac catcatcgcg gtcacgccgg cgccctgggc
       61 gatttcgcgg acgccaacgc cgtcataccc tgaccgggtg aacgcgattt gggccgattg
      121 cagtatcgcc tcgcgcgtgg ccgcgccttt cctgccctgc ttgggcgcgg cggcgccggc
      181 ctcttttacg cgcttgccac gtttcatgta taccactgta gactttttcg agcggctcgt
      241 ctacactgtg attgctgggt gagcgtcaag acggcgcacg gtcgtggaac cacctcagtt
      301 gagctgaact ggcggcgtag gcgctgaaat gggagcggta agcaggagac tgaaatggac
      361 ctgaaactga gtggcaaacg ggctctggtg acaggatcga gcgcgggcct cggcgaagcg
      421 atggtcaagt tgcttgcggc cgagggggtc gctgtcgtcg tccatggccg caacgcagac
      481 cgcacccata gagtggccaa cgcgatccac accgcgggcg ggcgggccga tgtcgctatg
      541 ggcgatctcg cgaccgacgc gggcgccgac gcggtgacta tggcggcgtt ggctggcgga
      601 ccgatcgata tcttggtcaa caacgccggt tgttaccacc acatgacctc gatggaagcg
      661 acgcctcgac tctgaacagg gagactgtaa tgccaaaagt ctggttgatc acgggcgctt
      721 cccgcggctt ggggcgggcg ctggccgacg cagtggttgc cggggggcac atgctggttg
      781 ccacagcgcg gaatcccgcg cagctcgctg atttggcact acatgccccc gatcgcattc
      841 gcactttcgc actggacgtg accgatgcag gggtggccga acgtgcaatt caatccgctg
      901 ttgacgcgtt cggtcggctc gatgtgctcg tgaacaatgc cggctacggc aatatcagct
      961 caattgagga gaccagcctt gtcgacttcc gcgagcagat cgaaacgaac ctgttcggca
     1021 cgatcaacgt gaccaaagct gcgatcccgc tgatgcgcga acaacgctcg gggcacatca
     1081 tccaggtttc ttcaatcggc gggcgtctca caccgcccgg ccgcggggcg tatgccacgg
     1141 ccaaggcagg cttgggcgcg ttctccgaag tactggcgaa ggaagtcggt ccactgggga
     1201 tcaaagtgac gatcgttgaa cccggcggct tccgcaccga ctttgccggc aagtcgacga
     1261 ccattcacga tagccgtccc gagtatgatt ccaccgtcgg ggcgatggcc cgataccagc
     1321 gagactacaa cggcaaacag cccggcgatc ccgccaaagc cgcggccgtc atcctgcaca
     1381 tcgccggcct ggatgaaccg ccgcttcgct tgttgctcgg caccgatgcc gccaacgccg
     1441 cggagaagaa aaacctggaa cggatcgaag ccgatcgccg atggcgcgac ctgagcgtct
     1501 ccacagactt tcatctgttg tgaataggga ccgcttagga tgcctggacc gcgccttcga
     1561 cggtgcggag gccaccttgg gaaccgtcct gcgtaaagcc cgcttctggg aagcccacgc
     1621 cggggagtcg ttcaacgacc gccaacgcag aatcgtctac cggctgctcg atggcttcga
     1681 gggcaagcca gatcgctgcc ctattgggta gcgcgacgct cgttcgacac acctaacgcc
     1741 ggccgtctga ttcgaccaca gccgagggag aatgacccca ggcggcgcag acaattcatg
     1801 ggcgaacgct ggtcagcgcg cgaatgatgg tctcgccgtc gcccctggtg taggtcccgc
     1861 caacagtcac gacgcgcccg caaaactcgt cgaagcgcgt gtcatccagg ttcccgaacc
     1921 gtgcctgcac cttgatcttc tcgccgtttg acagttgaag gtagtagatg ttgatctctt
     1981 cggccttatc actcgcccgc agaagtccgc gggcggtcaa ctggcgatcg gtgtcgtaga
     2041 cgactcgcca ggtcccgtca tcggtcacgc ccggcacggg acccagcgga cggtcaaggc
     2101 cggggagcgg cccggtgctc gacccgaaac gaggcgcgtc gatgcccagg cggtggagca
     2161 gtgacaacaa aaggttcgca tatggttgtg gctcggcata gcggatccag cgtcccgtct
     2221 tgagagtacc gccgcctccg cctgccagca cgacgggcag atcgcccgca gaatggtagt
     2281 cgccatgctt cagtcccgac ccgaacagta gcagagtgtt gtccagcagg gtcccgtctc
     2341 cttcctggat cttcgccagc cggtcgatca agtaggcgaa ctgggccacg tgccagcgcg
     2401 tgatggcgtt aaacctgtgg atgtattcaa cccccggatg gcggacgaag tgggacagat
     2461 agtgatgctc ttcgttgatg cccaggaagt cgaacacaag ccggctattg tcgttggcca
     2521 gcatgaatga ggcgattctc gtagagtccg tccagaacgc cagcaccatc aagttgagca
     2581 gggccttgac gcgttcgggt aagtccatca agaccggcgg ccggatcagt tccggcgcca
     2641 cccttggtcg ccaacggtcc ccatttggct ttcccgaggc cgcttcgatc gtccgttcga
     2701 cacctcgaac cgcatcgaaa taggcgtcga gcgtgcgccg gtcggcggta ctggccgaac
     2761 gccgcacact tcgggcgtct tcctgcactg catcgaggat gcttctggtt tgggagagac
     2821 gttccccgct tgagtatccg cggaacatgc gatcgaagac aatctgcgga tcgtcctcac
     2881 ggcggaccgg cttaccatca acgccatacg atatatagct gcatcgaggc tgagagagga
     2941 aaagactgtc tgccgtcagc tccagcgaac gtaagggcgt gtggcggccg attcggtcgg
     3001 cggccagctg atcgatcgtc accggttgcg ggacgtcgat cgaccccggg gtgttcgctc
     3061 ccgagaggaa cgcgacggcc gcggcatgat ggctaccgcc cccctcgcga tgtgcgaggt
     3121 tcgacaggat caataccttg tcgcgcatgg gcgcgagcgg ctccaagagc ggcgaaaggg
     3181 ggaagtccgt ctcgctggcc ccggagatgt cccaactcgg aggatggacc ccgttacctt
     3241 tgaacaggac cccgagccgc aacggcggct tcttggtcga atcggtcgcc gcgatggtgg
     3301 gcgacatgat ctcgagccag ggcaacgcca gccctacgcc catgccgcga agcaacctgc
     3361 gccgggagat cggatacgtt tcacccatgg cggccttttc tcgcctcgag gtcgtgttgc
     3421 cgtttttccc gtcgccctaa gtcaatcctc gcggtagccg aacgggacac tggcgacgat
     3481 ccccttgacg aggatcgaga aacggtagtc gttcgctttc aagcggtcga ccaggcggtc
     3541 aattgttggt cggtcggtat agaggattgc gcgaccgagg gcataggaga gcatccgctc
     3601 ggaggcgttg cggacgaatg gctcacggta ctgtgtggcc agcagggcct tcagctcggc
     3661 cgggccagac actcgctctc cgccgggcag tgtggtcgac gcatcgattg tcccaaattt
     3721 gtcgctcgtt ctccattcgc ccagcgggcc gaagttttcg agcgccaggc caaggggatc
     3781 gatcgcgtca tggcagcggg cgcagctcac ttcgccgcga tgcatctcca tttgttcctt
     3841 catcgagcga ggtcgccggc ccttcccggc ctcttctaga ggagggatgt tcggcggcgg
     3901 atcaggggga cgctggccga gaatcgcgtc caagatccag acgccgcgcc ggatcggact
     3961 ggttcgttcg ggggcggagg tggctacgag cgtggcggcc atgcccaata cggcgcctcg
     4021 gttcgggtcg tcaagcgcga cccgccgcag ggggtcgggc cagtcgtagc ctccgtcctt
     4081 gatcaatcgt tcacgtttat aacctttcag acgatagtgg tatcccagtg ttgggttcaa
     4141 gaatg
//
LOCUS       DATAQL010000836         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2224044, whole genome shotgun sequence.
ACCESSION   DATAQL010000836 DATAQL010000000
VERSION     DATAQL010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224044"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VHS97_14565"
     CDS             <1..752
                     /locus_tag="VHS97_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEX3449477.1"
                     /translation="GDLIDGLNNRLTQQIARAFRYDCHSENGEPDVEARAQSIVIRFL
                     DTLPELRHALAEDVEAAFDGDPAAKSPDEIVFCYPGVGAVTVYRMAHELHRLEVPLIP
                     RMMTEYAHGKTGIDIHPGATIGRRFFIDHGTGVVIGETTRIGEGVKLYQGVTLGALSF
                     PRDEKTGQIVRDAQRHPTIGDGVVIYANATILGGETVIGHHSVIGSSAWITRSIAPYT
                     TVTIENPRLRYRESNDGITDAEYLKRLNYQI"
     gene            complement(796..2091)
                     /locus_tag="VHS97_14570"
     CDS             complement(796..2091)
                     /locus_tag="VHS97_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX3449478.1"
                     /translation="MTAPFNSFQDEDEALPPVPQDEPESRPGGLPLYVWVILAVAIAV
                     PVGLFWGEGATNLELLPKLILRALTALAAPLVVLAILSAIVTNEVKGRLGVLMMLFYL
                     INTLVAMLIGLSLSNLLQPGKGASLVEPGTAAPTLAKKTATELLMDLIPRSIGEPFTQ
                     NHLAQLVLLTLALGIGLVKIRDSQKARGEKTFSTVVDLLTIGFELLMKVLLWVVALVP
                     LAVFGIVASSVGQKEGMRVFASLIWLIVVVVMGLSMQVVWYLFQMWAFARMSPLRFLR
                     GSLNVMANTISTASTAATIPITLRALQRLGISRRSSQLTACIGTNFNNDGTALYQATA
                     VLFMAQALGYHLSVLDQIMIMLTTLVASVGAGGIPSGSFVTMPLIFSAVRLPSDKIPI
                     LLTVDWLLDRCRTTSNVLGDMTVAVLLDRVSPPGEILDV"
     gene            2279..3070
                     /locus_tag="VHS97_14575"
     CDS             2279..3070
                     /locus_tag="VHS97_14575"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEX3449479.1"
                     /translation="MKIVDDRLARDSQKMSCLVLEHRAADGPANMALDEALLEMVAAG
                     LGAAFVRTYGWTIPTLSLGYFQNIAQVRADPRFQSVPLVRRLTGGGAIWHHHEITYAL
                     VVPAAHPLARPSTGLYNAVHGAIVEAMLELGISAARRGEFRQMVVGDRRRPLLCFTDP
                     DPEDIVTKGVKIVGSAQRRRGGAVLQHGSFLLARSYRTPELLGVSDVAGESAGHYDWA
                     ERIVKRIVNGLDLEPQAADIPAKVAARAAELEVARYRNPAWIALR"
BASE COUNT          652 a          901 c          967 g          609 t
ORIGIN      
        1 tcggcgacct gatcgatggg ttgaataacc ggctcacgca acaaatcgcc cgcgcattcc
       61 gctacgattg ccactcggaa aacggcgaac ccgatgtgga agccagggcc cagtcgatcg
      121 tgatccgctt tttggatacg ctgcccgaac tgcggcacgc tctggctgag gatgtggaag
      181 cggcgtttga cggcgacccg gccgccaaga gtcccgacga gatcgtcttt tgctatccgg
      241 gcgtcggagc cgtcaccgtt tatcgaatgg cgcacgagct tcatcgtctc gaagttccac
      301 tcatcccgcg catgatgacc gaatacgccc atggcaagac cggcatcgat atccatcccg
      361 gcgcaacgat tggccgccgg tttttcatcg atcatggcac cggcgtcgtc atcggtgaga
      421 ccaccaggat cggcgagggg gtcaagctct accaaggcgt cacgctgggc gctctcagtt
      481 tcccacgcga cgagaaaact gggcagatcg tgcgcgacgc tcagcggcac ccaaccatcg
      541 gcgacggtgt ggtcatctac gcgaatgcca cgattttggg cggcgagacg gtcattggtc
      601 atcattcggt gatcgggtcg tcggcctgga tcacgcgcag catcgctccg tacacgaccg
      661 tcaccatcga aaaccctcgg ctccggtacc gcgaatcaaa cgatggcatc accgatgccg
      721 agtatctgaa acggctcaat tatcagatct gagaacgtga caaccttttt tccctggatt
      781 ggtctgggtt gacggtcata catccagaat ctcgcctggc ggtgataccc ggtcgagcaa
      841 tacggcgacg gtcatatcgc cgagcacatt ggaagtggtc cggcaacggt ccaagagcca
      901 gtcgacggtg agcaagatcg ggatcttgtc ggaaggaagc cggacggccg aaaagatcaa
      961 gggcatcgtc acaaagctcc ccgagggaat tccgcccgcc ccgacgctgg ccacgagcgt
     1021 tgtcagcatg atcatgatct ggtcgaggac gctcaagtgg taaccgagag cctgagccat
     1081 gaaaaggacc gcggttgcct ggtaaagggc agtgccgtca ttgttgaagt tggtgccgat
     1141 gcaggcggtc aactgactcg atcgccgcga aatacccagc cgctggagcg ctcgcaacgt
     1201 gatcgggatc gtcgcggccg tgctggcggt gctgattgtg ttggccatca cgttcaaaga
     1261 ccctcggaga aaccgcagcg gggacatccg cgcgaacgcc cacatctgga acaagtacca
     1321 tacgacctgc atcgagagcc ccatgacgac cacgacgatc agccagatca aagacgcgaa
     1381 gaccctcatc ccttcctttt ggcctacgct cgaggcgacg atcccgaaga ccgccagcgg
     1441 tacaagagcc acaacccaga gcaagacctt catcaagagc tcgaagccga tcgtgagcaa
     1501 gtcgaccacg gtgctgaacg tcttttcacc acgagccttc tggctgtccc tgattttcac
     1561 caggccgatg ccaagggcca gcgtgagtaa gaccagttgc gccaggtggt tctgggtgaa
     1621 gggctcgccg atgcttctgg ggatcaggtc catgagaagc tcggtcgccg tcttcttggc
     1681 gagggtcgga gcggcggtcc ccggctctac cagtgacgcg cctttaccgg gctggagtag
     1741 attcgacaaa ctgagaccga tcagcatcgc gaccagggta ttgatcagat agaacagcat
     1801 cataaggaca ccgaggcggc ctttgacctc attggtgacg atggcactca agatggcgag
     1861 tacgaccagc ggggccgcca acgcggtcag ggcccgcagg atgagcttgg gcaggagttc
     1921 gaggttcgtc gcgccctcac cccaaaagag ccccacggga accgcgattg ccaccgccag
     1981 gatgacccaa acatagaggg gcagccctcc gggacggctc tcgggttcgt cctgtggcac
     2041 cggtgggaga gcttcgtctt cgtcctgaaa actattaaag ggcgcggtca tcgcggactc
     2101 cggtaagtaa gttcaggcgt ctggggcgac ttgctggttt ctgtcacttg cagtggaatg
     2161 tcgaattatg gcggcaagtc ttacaagttg cgagtggccg ggctcccttg ggtcggcggt
     2221 aggaccagcc gctgattttg cgaatgggcg gtcgagatca agaaacttgg atgaatcggt
     2281 gaaaatcgtg gacgatcggc tggcgagaga ttcccaaaaa atgtcttgcc tggtgctcga
     2341 gcaccgcgcg gcggacgggc cggcgaacat ggcacttgac gaggccctgc tcgaaatggt
     2401 ggccgccggc cttggcgcgg ctttcgtgcg cacgtacggg tggacgattc cgactctgag
     2461 cctgggctac ttccagaata tcgcccaggt tcgggccgat ccgcggtttc aatcggttcc
     2521 gctggtgcgc cggttgaccg gcggcggtgc gatctggcat caccacgaga taacttacgc
     2581 gctggtcgtg ccggccgctc atcctctggc tcgacccagc acaggattat acaacgcagt
     2641 gcacggggcc atcgtggagg ctatgctgga gttgggaatt tcggctgccc ggcgcggcga
     2701 attccgccaa atggtcgttg gcgatcgaag acgaccactt ttatgcttta ctgaccccga
     2761 tccagaagat atcgtaacca aaggcgtcaa gatcgttggc agtgcgcaac gtcggcgtgg
     2821 aggcgctgtg ctccagcacg gttcgttttt gttggctcgg tcgtaccgga cgcccgagct
     2881 tctcggtgtg agcgatgtcg cgggcgaatc ggcggggcat tatgattggg cagaacggat
     2941 cgtcaagcgt attgtcaatg gcttggattt ggagcctcag gcagccgaca ttccagccaa
     3001 agtcgcagcc cgggccgcgg aactggaagt ggcacgttac cgcaatcctg cttggatcgc
     3061 tcttcgctag taacggcagt tcgtgtcagt gtcatcctta caatcatcca aagagtgaat
     3121 cgcgggttg
//
LOCUS       DATAQL010000837         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1767399, whole genome shotgun sequence.
ACCESSION   DATAQL010000837 DATAQL010000000
VERSION     DATAQL010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767399"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="VHS97_14580"
     CDS             <1..2047
                     /locus_tag="VHS97_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3449480.1"
                     /translation="LGKTGTKLEDLPTSVQIINRDVINQQGGATVLDASTNASGIVQG
                     AEDTHYDFFLIRGLNAQFYNDGFRDGDQLVGLSHSLNGVKQVEILEGPGSALFGSGPP
                     GGTINIVHYTPSSDFHYGASVQTGSFGTINSTGYVTGPTSIPGLNYRIDGTASHTEGF
                     RDLKGSDYEIRPDIVWNVNNHTLEFSLDARQLNQTPDSYGIIYFNGSPLKSVPVDTRY
                     TWPFNHADQNYIRPTATDKWWVSDYLTINNRLSYLYRDFGTMRTGDSSSTHVDTNPAS
                     PTFNQVVGMQIRNQHDRDSILDYEFEPVWKFATGPVTHTLLTGFEYIHEIIDTQRTTA
                     DLPNIANAFAPVPPMLLPSQLAFQCGKVVSAVTGATASHSCDDDHLAADYYSLYATDQ
                     VDVTDRLKVRAGVRQDWWNTALTPLINVPGGTFNNEGVPLTAGVTQERNDTPVSWNVG
                     ALYKLFPGVSPYVGASKSYLSNFNSENVQTGIGAPESALQYEAGVKFSLLNDRVVLNT
                     AVFDVKRDNVAAPLTIGGVESVVFDSQRTRGYEAALDARVTDQWRILANFTAQDAVIT
                     DNPQGITSVGNHPQSVPAYMANLWSTYRFSIAGVPGFIVGAGLNYRDKNYSDITNVNS
                     IPAFVIGNALFGYEADTWGISLNVKNFTNQRYYVEANAAGAAVGESLSAFLKVYIKQ"
     gene            complement(2344..>3503)
                     /locus_tag="VHS97_14585"
     CDS             complement(2344..>3503)
                     /locus_tag="VHS97_14585"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX3449481.1"
                     /translation="EVDTLMAELRRTAARRQAAEDWLRESKDRLQTALNVAQLGSYRY
                     DPHHRVFSGDTRSQEIFDFPKNDFPKNEAAIEEIMKLVHPDDVAMVQANLEASLDPVD
                     PRLSATEFRIRRGDGEIRWVETLGLAYFEGIGNERRAVSFVGTLQDITERKKREEERR
                     EREEKEHLLMREINHRAKNMLSVVDAIAHQTATRNPEDFVKRFSERIQALSANQDLLV
                     RNEWNGVEIEDLVCAQLAPFADLIGSRIAVQGPKLRLKAASAQAIGLALHELATNAGK
                     YGALSTDTGRVDVRWGTDGDTFAMSWTERDGPPVSAPKRRGFGTIVMEAMTERSVDGT
                     VDLDYAPSGVTWRLTCPAANALEPWEREQNSGERENRTNGTTGKARCETPA"
BASE COUNT          697 a         1101 c         1001 g          704 t
ORIGIN      
        1 tctcggtaag accgggacga agctggaaga cctgcccacg agcgttcaga tcattaaccg
       61 cgacgtcatc aatcagcagg gcggcgccac ggtccttgac gcgagtacga atgcgagcgg
      121 cattgtccag ggcgccgagg acacgcacta cgacttcttc ctgatccgcg gcttgaacgc
      181 ccagttctac aatgacggtt tccgcgacgg cgaccagctc gtcggccttt cccactcgct
      241 caacggcgtg aagcaggtcg aaattctgga agggccgggt tcggcgctgt tcggcagcgg
      301 accgccgggc ggcacgatca acatcgtcca ttataccccg tcgtcggatt ttcactacgg
      361 cgccagcgta cagacgggct cgttcgggac catcaatagc accggctatg tgactggccc
      421 cacgtcgatt ccgggcctga actatcgcat cgacgggacg gcctctcaca cggaggggtt
      481 tcgcgatctc aagggttccg attacgaaat caggccggac atcgtctgga acgtgaacaa
      541 tcacacgctc gaattcagcc tcgatgcccg ccagctgaac cagacgccgg attcctacgg
      601 catcatctac ttcaacggct cccccctgaa gagcgtccca gtcgacacca ggtacacgtg
      661 gccgttcaac cacgccgacc agaattacat tcgcccgacg gctacggaca agtggtgggt
      721 cagcgactac ctgaccatca acaaccggct gtcctatctg tacagagatt tcggcaccat
      781 gcgaaccggc gacagctcga gcacgcatgt ggacaccaac ccggcttcgc cgacgttcaa
      841 ccaggtcgtt ggcatgcaga ttcgcaacca gcacgatcgc gacagcatcc tcgattatga
      901 gttcgagccg gtgtggaagt tcgccaccgg ccccgtgacc cacaccctcc tcaccggctt
      961 cgaatacatc cacgagatca tcgatacgca gcgcacgacg gcggaccttc ccaatatcgc
     1021 caacgccttt gcaccggtcc caccgatgct attgccgagc cagctggcgt tccaatgcgg
     1081 taaagttgtc agtgctgtta ctggggctac tgcgagccat tcatgtgacg acgatcatct
     1141 ggccgcggat tattacagcc tctatgccac cgaccaggtt gacgtgaccg acaggctcaa
     1201 ggttcgcgcc ggcgtccggc aggactggtg gaataccgcg ctgacgcccc tgatcaacgt
     1261 gcccgggggg accttcaaca atgaaggcgt acccctcacg gcgggcgtga cgcaggagcg
     1321 caatgacacg ccggtgagct ggaacgtcgg cgcgctgtac aagctttttc ccggcgtttc
     1381 gccgtatgtc ggcgcgtcaa agagctatct ttccaacttc aactctgaaa acgtccagac
     1441 gggaattggc gcgccggaat ccgcattgca atatgaggcg ggcgtcaagt tctcgctcct
     1501 caatgacagg gtggtcctga acacagcggt cttcgacgtt aaacgcgaca acgtcgccgc
     1561 ccccctgacg atcggtgggg ttgaatcagt cgtgttcgac agtcagagga caaggggata
     1621 cgaggccgcg ctcgatgcga gggtaacgga tcagtggcga atcctggcca attttacggc
     1681 tcaagacgca gtgattacgg acaatcccca aggcatcacc tcggtgggca atcatcccca
     1741 gagcgtcccg gcctacatgg cgaacctgtg gtcgacctac aggttttcca tcgcgggcgt
     1801 gcccggtttc attgtcggcg cgggcctgaa ttaccgggac aagaactaca gcgacatcac
     1861 gaacgtaaac tcgatacctg ccttcgtgat cggaaatgcc cttttcggct atgaagcaga
     1921 cacctgggga atttcgctga atgtgaagaa cttcaccaat cagaggtact atgtggaagc
     1981 caatgccgcg ggcgcagccg tcggcgagtc attgagcgct tttcttaagg tctatatcaa
     2041 acagtagccg cgggaatgcc cgcgctcgac atcccctccg ctacacgggc ccctggctgg
     2101 catcctcgcc ttgggtattc gtgctcgcga tgcgcggttc gaaagctggc aacactggcg
     2161 acatcctgcc gcgatagctt tgccgcgtct gagggctgct gcgggtcaag tgttgccgtc
     2221 gcgaccaagt tggcagcacg gccggtgtac cccagcgacc tatcgcgtcg ctcaactcgg
     2281 cagccttggg ccaataccag acatccaaac atatgcgagc tgaatctgga gctaaccacg
     2341 agttcaagct ggtgtttcgc acctcgcctt gccagtcgtg ccgttagttc gattttctct
     2401 ttcgcctgaa ttttgctccc gttcccaggg ctccagcgcg ttcgctgctg ggcaggtcag
     2461 gcgccaggtc acgcccgagg gcgcgtaatc aaggtcgacc gtgccatcca cgctgcgttc
     2521 cgtcatcgct tccataacta tggtgccgaa cccgcgccgt ttcggcgcag atacgggcgg
     2581 gccatcgcgc tcggtccagc tcatggcgaa ggtgtcgccg tcggtccccc agcgaacatc
     2641 gacacgacca gtatctgtcg agagcgcccc gtacttcccg gcgttggtag cgagctcgtg
     2701 cagtgcgagc ccgatggcct gcgcggaggc cgctttcaag cgcagcttgg ggccttgcac
     2761 ggcgatacga gaaccaatga gatcggcgaa gggcgcgagc tgggcacaaa ccaggtcctc
     2821 gatctcgacc ccattccatt cgttgcggac cagaaggtcc tgattggctg aaagcgcctg
     2881 gatgcgctcg gagaagcgtt tgacgaaatc ttcgggattt ctggtggcgg tctggtgagc
     2941 gatcgcgtcc acgacgctca gcatgttctt ggcgcgatga ttgatttcgc gcatgaggag
     3001 gtgctctttt tcctcgcgct ccctgcgctc ttcctcgcgc ttcttgcgtt cggtaatgtc
     3061 ctgtaaggtg ccgacgaagc tcacggcccg ccgctcattc ccgatgccct cgaaatacgc
     3121 aagccccaga gtctccaccc agcggatctc gccgtctcct cgcctgatcc ggaactcggt
     3181 tgcggagagc ctcggatcga caggatcgag cgacgcctca aggttcgctt gaaccattgc
     3241 cacgtcgtcc ggatgcacca gcttcatgat ctcttcaata gccgcctcat tcttgggaaa
     3301 gtcgttcttg ggaaaatcga aaatctcctg agagcgcgta tcccccgaga acacgcggtg
     3361 gtgcggatcg taccggtacg agccgagttg ggctacgttg agagcagtct gcagacgatc
     3421 tttactctcg cgcagccaat cctctgctgc ctgtcgcctg gcggcggtcc gtcgaagctc
     3481 cgccatcagc gtgtcgacct cag
//
LOCUS       DATAQL010000838         4773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3139382, whole genome shotgun sequence.
ACCESSION   DATAQL010000838 DATAQL010000000
VERSION     DATAQL010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3139382"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VHS97_14590"
     CDS             complement(<1..333)
                     /locus_tag="VHS97_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449482.1"
                     /translation="MSWLRRAWRWGRNAIVVGSIALAAFLTATHEMGAWPWNDRRPLR
                     DRFLFTHVSRTDLNPILNAPGRLESSKKTLIRCELENLVAGARGSGSSTILTLLPEGT
                     VVKPGDVLA"
     gene            767..4177
                     /locus_tag="VHS97_14595"
     CDS             767..4177
                     /locus_tag="VHS97_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3449483.1"
                     /translation="MTRPAALCLTLVFGIQSLASAREAKLVRYPDYHGGRVAFAYLGD
                     IWTADEDGKNIQRLTVHTARDIHPRFSPDGQFLAFSSDREGNMDVYVIPTTGGAVKRL
                     TIHSADDTVLDWTPDGKGILFASQRGEDFMGKLYVVSIDGGLPRDAGSDMGVTGSFSP
                     DGTKLAVNRKAQSYWRKYYRGAYQSDVTILNIATKTFKDLTHFEGMDSWPLWSRDGFI
                     YFVSDREGKGQTNIWRVGEAGGDAEQVTKFTGGDVRFPGMSGDGKTIVFEHDFGIWKL
                     DVATRNVKTIPLEIAAETQETLTEFRNFDSTIDDYDLAPDGKRIVISVHGEVFTAPTD
                     EGELRQLTEGASRDLDVQYSPDGKSIAFVSDQSGREEIHVIAADGAGASRKVTDLNTL
                     KTSLAWSPDSKSIAFVTSDRTLITIGSDGKNLKELASSNHGPIGNPAWSPDGKLIAYS
                     KTDVSRASDIYLIPSTGGEEKKITFDSASESNPRFSADGSKVYFVRREGDGGGETRPS
                     SQLFCVPLEKLNKDPDEPEQRPDGSSSEGGQEMRRPAMARPVTPKTPTIDWAGLKRRT
                     RQVTRTSSVFTYVPGTDGKTVIFVGSEGSAGGGGGPGGFGGRGGGGTPSIYTIQDNGK
                     RMTRIATGTPRPTVGDDEDRPRGMRGGFRGGISNLRLTKDGRTLYFQEGESVYSTPIG
                     GGGGGGGGAGMIAALSGGGPRGSGESSGVARGGGDGGAKKKINFNVTVRIDKPQEWDE
                     MFDDAWRCMKYRFYDPKLHGTDWDAMRAKYKPLVAYVADRHELMNVINEMIGELNASH
                     TGATAGRDRGQAAGEGPGIMTRHLGLDFQADEASGRYKVTHIYEDGPADKDWIKVEKG
                     NYLLAIDGKPVKAGDDYAKFLGRRLNPKVELTFNTKPAPEGAWTVKYEPIPLPAYGNL
                     RYNRWVNERRAMVEKLSGGRVGYLHIKAMDQPSLTKFKKELGEFRHKEGLVIDERFNG
                     GGNIEQELLAILVQRPYEVWQPRGLEPTERPFAGYFGPKVVLQNWRSASNAEMFPAGF
                     RALGLGKVVGTPTMGAVIGTGSYSLIDGSSIRTPGVGVFLADTARTNMENHAVQPDVY
                     VENTPEDNLAGVDRQLETAVREVMKELKPAESVAKRAKGASHNGGGME"
     gene            complement(4384..>4773)
                     /locus_tag="VHS97_14600"
     CDS             complement(4384..>4773)
                     /locus_tag="VHS97_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX3449484.1"
                     /translation="PIGGDLLTGLVQAGLVLVALRHYKVPRTIAARMMANVLLDVAVG
                     SIPILGDLFDVAFKANTRNIKLLEPYGLESTIAGEHSRHPGSHMIDLKPRGTPWRYIV
                     PIAAVLIIALTLVLIGFITVVRWLFGF"
BASE COUNT         1032 a         1429 c         1472 g          840 t
ORIGIN      
        1 cgccagcaca tcgccgggct tgaccacggt tccttcgggc agcagcgtta gaatcgtcga
       61 agacccgctg ccgcgcgccc cggcgaccag gttttcgagt tcgcaacgga tcaaggtttt
      121 tttcgagctt tccaatcgac cgggggcgtt caatatcgga ttcaagtcag tgcggctgac
      181 atgtgtgaac aaaaagcgat cgcgaagcgg gcggcgatcg ttccaaggcc atgcacccat
      241 ttcgtgagtc gcggtcagga aagccgccag agcgatcgat ccgacgacga tcgcgttgcg
      301 tccccagcgc catgcacgtc gcagccagct catctagtga gcctcctcgc tcggttgacg
      361 gtgaccaaag gttggaggtt tcaagccaac tcgatcacgg agcttgtttg ctttccccac
      421 actgttaaag cgtcaagaag agctgcgctg gccaggggaa gggacgaacc catagagaag
      481 atgctgataa actccgacgg cgccttagag caccagtcaa ggaaagcggt tgtgacgatg
      541 aatccggcag agcgacgatc ggcccgttga gcctgatttg gcaatgatcg gtagcgcaaa
      601 cgatgctttt tgcctgcgca cgccagtcca tcgaactcaa atgacggtcg agcgagcaat
      661 ttcgggccag agcgtcgtcg aatgtcgcct aattctttga taacattcta gccgacccgg
      721 caccaaatcg tcgtcggggt ttgaacatcg attccggaac cacaccatga cgagacccgc
      781 tgcgctttgc ctcaccctcg tttttgggat ccagagttta gcctcggccc gagaggcaaa
      841 gctggttcgc tatccggatt atcacggcgg cagggttgct tttgcctacc tgggggacat
      901 ttggaccgcc gacgaagacg gcaagaacat tcagcggctc accgtccaca cggcgcgcga
      961 catccatccg cgtttttcgc ccgatggcca attcctcgcg ttttcctccg atcgcgaggg
     1021 aaacatggac gtttacgtga ttcccacgac cggcggagcc gtcaagcgcc tgacaattca
     1081 ctcggccgac gacacggtcc tcgactggac ccccgacggc aaggggatct tgttcgcgag
     1141 ccagcgcggc gaagatttca tgggtaaact ttatgtggtc tcgatcgacg gcggtcttcc
     1201 gcgggatgcc ggcagcgaca tgggagtcac cggctcgttc tcacccgacg gtaccaagct
     1261 ggccgtcaac cgcaaggctc aatcgtactg gcgcaaatac taccgaggtg cctatcaaag
     1321 cgatgtcacc atcctgaaca tcgccaccaa aaccttcaag gatttgaccc acttcgaggg
     1381 aatggattcg tggccactct ggagccgtga cggcttcatc tactttgtca gtgaccgcga
     1441 aggcaaaggt caaaccaaca tttggcgggt gggagaggcc ggtggtgacg ccgagcaggt
     1501 gacaaaattc accggcggcg acgtgcgctt tccgggaatg agcggcgacg gcaaaaccat
     1561 cgtctttgaa catgatttcg gtatctggaa gctcgacgtt gcgacccgga atgtcaaaac
     1621 catcccactc gagattgcag ccgaaacaca agagacactg accgaattca ggaacttcga
     1681 ttcgacaata gacgattatg acctcgcccc ggatggcaag cggatcgtca tcagcgtgca
     1741 cggcgaggtt ttcaccgcgc cgaccgacga aggtgagctc cggcagctca ccgaaggcgc
     1801 ctcccgcgac ctggacgtcc agtattctcc ggacggcaaa tcgatcgcat tcgtctcgga
     1861 ccagagcgga cgcgaggaga tccacgtcat tgccgccgac ggcgcgggcg cctccaggaa
     1921 agtcaccgac ctgaataccc tgaagacatc tcttgcctgg tcacccgaca gcaagtcgat
     1981 cgcgtttgta acgtcggatc gcacgctgat cacgattggg tccgacggta aaaacctcaa
     2041 ggagctggcg tcgtcgaacc acggaccgat cggcaatccg gcctggtcgc cggatggtaa
     2101 gctcatcgct tactccaaga ccgacgtcag ccgcgcgagc gacatctatc tgattccgtc
     2161 cactggcggc gaggagaaga aaatcacgtt cgactcggca agcgagagca atcctcgttt
     2221 ttccgccgat ggctccaagg tctacttcgt ccgccgcgag ggggatggcg gcggcgagac
     2281 gcgaccctcg tcgcaactct tttgcgtccc gctcgaaaag ctcaacaagg atcccgacga
     2341 gcctgagcag cggccggacg gttcttcgtc cgaggggggc caggagatgc gcagaccggc
     2401 gatggcccga ccggtcacac ccaagacgcc gaccatcgat tgggccggcc tgaagcggcg
     2461 cactcgccag gtcacgcgaa cgtcgtcggt tttcacctac gtccctggca ccgacggcaa
     2521 aacggtgatt ttcgtcgggt cggaagggag tgctgggggc ggcggtggcc cgggcggatt
     2581 cggcgggcgc ggcggcggcg gaactccgtc gatctacacg atccaggata atggcaaacg
     2641 gatgacccgc atcgccaccg gtacgcccag gcccacggtc ggcgacgacg aagatcgccc
     2701 tcgaggcatg cggggcggtt tccgaggggg aatctccaac ctccgactca ccaaggacgg
     2761 ccgcacactc tatttccagg aaggcgaatc ggtctacagc acgcctattg gtggcggtgg
     2821 cgggggtgga ggaggcgcgg gaatgatcgc agcgctaagc ggcggcggcc cacggggctc
     2881 aggtgaatcg agcggcgtcg ccagaggcgg cggcgacggt ggcgcgaaga agaagatcaa
     2941 cttcaacgtc accgtccgga tcgacaagcc gcaggaatgg gacgagatgt ttgacgacgc
     3001 ctggcgttgc atgaagtatc gcttctacga tcccaagctg cacggcaccg actgggacgc
     3061 gatgcgggcc aagtacaagc cgctggtcgc ctatgtggcc gatcggcacg agctgatgaa
     3121 cgtgatcaac gagatgatcg gcgagctgaa tgcttcgcac acaggcgcca cggccggccg
     3181 cgaccggggc caggcggcgg gcgaagggcc cggcatcatg acccggcatc tcggcctcga
     3241 tttccaggcg gacgaggcca gcggtcggta caaggtcact cacatctacg aagacggacc
     3301 agccgacaaa gactggatca aggtcgaaaa gggcaattac ctgctcgcca tcgacggtaa
     3361 gccggtcaag gccggcgacg actacgccaa gttcctgggc cgccggctca atcccaaggt
     3421 cgaattgacc ttcaatacca agcccgcgcc cgagggagct tggaccgtga agtacgagcc
     3481 gatcccgctg ccagcctacg gcaacctccg ctacaaccgc tgggtcaatg agcggcgcgc
     3541 catggtcgaa aaactttctg gcggacgggt cggctacctg cacatcaagg cgatggatca
     3601 gccttcgctg acgaaattca agaaagagtt aggcgagttt cggcacaagg aagggctggt
     3661 catcgacgag cgctttaatg gtggcggcaa catcgagcag gaactgttgg caatacttgt
     3721 gcagcggccc tatgaggtct ggcaaccgcg cggactggaa ccgacggagc ggcctttcgc
     3781 cggctatttc ggcccgaagg tcgttttgca aaactggcgc agcgcctcca atgccgagat
     3841 gtttcccgcc ggcttccgcg ctctgggcct gggtaaggtg gtcggcaccc caacaatggg
     3901 ggcagtcatc ggcacgggta gctattctct gatcgacggc tcgtcgatcc gcacgccggg
     3961 cgtcggcgtc ttcctcgccg acaccgcgcg aaccaacatg gaaaaccacg ccgtccagcc
     4021 cgacgtttat gtggaaaata ctcctgaaga caatctcgcc ggcgtcgacc gccaactcga
     4081 aacggccgtc cgcgaagtga tgaaagaact gaagccggcc gagtcagtcg ccaagcgcgc
     4141 caaaggggca agtcacaatg gcggcggcat ggaatgaact gttccggcca atagacgccc
     4201 gctgatcgcc gcgcaagagc gctgcgatga tctgtcagca tacggttcgg ccgcggattc
     4261 gcagcccgtt tatcccagta cgatccggtc cagttcgacc attcgccgtg aacttggctc
     4321 ttcctcgccc accgcatgga agatgacggt gtcggcgggt gagatcggcg cccagttgtt
     4381 tcgtcagaac ccaaatagcc agcgaacgac ggtgataaag ccgatcaaga cgagggtcaa
     4441 agcgatgatc agcacggcgg cgatgggcac gatgtatcgc cacggagttc cgcgcggctt
     4501 gaggtcgatc atgtgtgagc ccggatggcg ggagtgctca ccggcgatcg tgctctcaag
     4561 tccgtaaggc tccaggagtt tgatgttgcg ggtatttgcc ttgaacgcaa catcaaacag
     4621 gtcgcccagg atgggtatcg acccgacggc gacatcgagc aggacgtttg ccatcatgcg
     4681 ggcggcgatg gtacggggta ccttgtaatg gcggagagcg accagcacca gccctgcctg
     4741 aacgagcccg gtcagaagat cgccgccgat cgg
//
LOCUS       DATAQL010000839         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4056456, whole genome shotgun sequence.
ACCESSION   DATAQL010000839 DATAQL010000000
VERSION     DATAQL010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4056456"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1297)
                     /locus_tag="VHS97_14605"
                     /pseudo
     CDS             complement(162..1297)
                     /locus_tag="VHS97_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          301 a          534 c          479 g          363 t
ORIGIN      
        1 cgagattcgc gaatgccgat gtgttttctg acgtgttcac ccggacaaaa ctgaaccaca
       61 agggcgatcc gctcacaacg attggcggat cgatgcgggt gcagggtgaa gtgcgcccct
      121 aagtttggac acgaaaacgg cggggctttt ggaacatgca ctcacgcacg cgcggagtgc
      181 ttctctctcc aatggctctc gaactcggcc ggggtcaggt agcccagcga cgagtgaatc
      241 cgcttgcgat tgtagacatc gtcaagaaac ctcccaaggc tcctcagcac atcgccgaag
      301 tcctcgtact ccgaaaggtc gacctcctct tccttgatgg tcctcatcag cctctcggcg
      361 tagccgttct gctcgggttc gcccacgctc gccatgctga tcttcacctt gaggctcttc
      421 agcatctcga cgtaggccgt cgcggcatat tgcacccctt ggtcggaatg gtggatctcc
      481 gggcggccat gctcgtacgc ccgcctgagc gccctgaggg tcaactcctg gtcgagcccg
      541 cgccccaggt gccagccacg gatgcagcgg gtgtagacgt ccatcaacac cgctaaatag
      601 acaaactcct tcctcaatcg gatataggtg atgtcggcca cccagacctc gtcggggcgc
      661 gtcacctcca ggctctccac gaggttggga tagcgcggat acgcgtgacc gctatccgtc
      721 gttcgaggcg tcttttccgg ggctttcccc acaatcccca gcgcgtgcat caaccggcgc
      781 acccgcttgg cgttgacggc gtgaccctcg cgctggagct gcttggtcaa ccgccgatag
      841 ccatacgtcg gccattgacc ggccaccttg accagggcat ccagaatgag ccggtcatcg
      901 ctcggctggg gcccgtagta cgcgctgctg cgtggcaggt cgagcacgtc gcaaagccga
      961 ttgatcggat agtgttcacg caacaacaac atcatttctc gcttctcgtc gagagtgagc
     1021 tcagcctgct cgaggctttt tttaggatct cgttctccag ggtcatgcgc cccagaacct
     1081 gttccagttc ggcgatccgt gcctgctcgg tcgagcgcgc cgaatcgctg tcgaagacca
     1141 tgtgtgcctt ctcgaggaag gccgtcttcc acaaggccag caggtttggc cccaggctgt
     1201 gcttgcgaca agcctccgcc tgagactgaa cgcccgcgag aacgtcaagc accactcgcg
     1261 ccttgaactc cggagtaaac tgacgtcgct ttctcatccc gatgcgccct ccgaactgga
     1321 tctgcccctc cattatgcac ctgtccagtt ttcggggcgc actacagttc gaatcctccc
     1381 gacgttggtg atcgccgagt ttcgtggcgg ctctctgtct tggtcgcgtc cgtctccttg
     1441 tgggacgagt gccgccgagg cagggagccg cttgctcaca ggcgatagtg ataacttttc
     1501 tttgcctttt cttttagcta tcggcgatcc tcaagcaagg ctcgaaattg gtagtggcta
     1561 ggggggcgac tcgaagtggg aaacgacctg cggctgagct cagcccttgc cacctgtgcc
     1621 gccgaacgca cctgacggtg ctcgccgcag tggtgtgtct gaagcggaac actgtag
//
LOCUS       DATAQL010000840         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3599962, whole genome shotgun sequence.
ACCESSION   DATAQL010000840 DATAQL010000000
VERSION     DATAQL010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3599962"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..>1573
                     /locus_tag="VHS97_14610"
     CDS             321..>1573
                     /locus_tag="VHS97_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3449485.1"
                     /translation="MATATFVVIAGMVGAGILTTSGYTIALVGSNQWMLILWVLGGVT
                     AVCGALTLAELSAAMPRTGGDYVYLYEAYGPLPAFLSGWVSFLIGFAGPSAASAFAFA
                     KYTLAPFQSPGTDATFRERVLATIAILVFAGIHVSSRRQTARVQGWITMLKITCLVAF
                     ALSGLSIGWPNAANLADRTPLDGKLAVTLMSSMVYIYYAYTGWNSASYLAGEVKDAQR
                     RLPQAILLGTASVTVLYLALNVVYGLALSAADIGQIVSDPSNHEGLEAVAPIAQLAAA
                     RLFGVRWSMPLSFTIGLMLLSTLSAYVLIGPRVVYAMAKAGQFPAFAARLTRRAGTPV
                     IATALQVAVALLILWTGTQEKIIVYSGVGLSIFSMLAMSSIYILRWRRPDLPRPFRTP
                     GYPVTPAIYLILTLLLTLATFHDRP"
BASE COUNT          290 a          482 c          429 g          372 t
ORIGIN      
        1 acaccgaacg cgagaccgtt ctcggcctct tctcagggtc cgaagctcgc tcacaggaaa
       61 cccctaactt ccccgttgtc acctcattga aagaaatcca tccggtttat ttacgagcat
      121 cctctcgata gtcattcttg gtcgcgactc tgccactttc cacacgttca tcgaacttta
      181 atcgaattaa gagccatttt acccatagta cgacattttt ccatctttcg ctggcaacca
      241 cgatgaccga atcgaatcag ccgacggaaa aaccagaaaa acccgcagat tcacccgaga
      301 ctatgccggc cgagtttggg ttggcaacag cgacgtttgt tgtgatcgcg ggcatggtcg
      361 gcgccggaat cctaacgaca tcggggtata cgatcgctct ggtcgggagc aatcagtgga
      421 tgctgatcct ctgggtgttg ggtggtgtca cggcggtctg cggggcactg acccttgccg
      481 agctctccgc ggccatgccc aggacgggag gagactacgt ttatctttac gaagcgtatg
      541 gtccgttgcc ggcgttcttg tcgggttggg tctcgtttct gatcgggttc gccggtccca
      601 gcgcggcgtc ggcgtttgcg tttgccaaat acaccctagc accttttcaa tcgccaggca
      661 cggacgcgac gttccgcgag cgcgtcttgg caaccattgc catcctggtt ttcgccggaa
      721 tccacgtctc gagccggcgc cagacagcca gggttcaggg atggatcaca atgctcaaaa
      781 taacatgtct tgtggctttc gcattgtccg gcctgtccat aggctggcca aatgcagcca
      841 acctcgcgga tcgcacgccc cttgatggta agctggcggt gaccctgatg tcgtcgatgg
      901 tttacatcta ctatgcatac acgggctgga attcggcttc gtacctggca ggcgaggtca
      961 aggatgcgca gcgccggctt ccgcaggcga tcttacttgg aacagccagc gtgacggtcc
     1021 tatatctcgc ccttaacgtt gtttacggtc tggcgctttc ggcggccgat attggccaga
     1081 tcgtgagcga tccgtcgaac cacgaagggc tcgaagcagt tgcccccatc gcccagctcg
     1141 ccgcggcccg ccttttcggg gtcaggtggt cgatgccgct cagctttacc atcggcctca
     1201 tgcttctttc caccttgagc gcctacgttt tgatcgggcc gcgcgtggtg tacgcgatgg
     1261 cgaaggcggg ccagttcccg gcctttgccg ctcgacttac ccgacgagca ggcacaccgg
     1321 tgattgcgac ggcccttcag gtggctgtcg ccctcctcat cctctggaca ggaacacaag
     1381 aaaagatcat cgtctactca ggtgttggct tgtctatttt ctcgatgctg gcaatgagct
     1441 cgatctatat cctccgctgg cgacgcccgg acttgccccg accgttccgc acaccgggat
     1501 atccggtgac gcccgccatc tacttgatcc tgactctact gctcacgctg gcgacttttc
     1561 acgatcgtcc cag
//
LOCUS       DATAQL010000841         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1612603, whole genome shotgun sequence.
ACCESSION   DATAQL010000841 DATAQL010000000
VERSION     DATAQL010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612603"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1830
                     /locus_tag="VHS97_14615"
     CDS             139..1830
                     /locus_tag="VHS97_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3449486.1"
                     /translation="MMRTAPRASLHCAVYTRVSTDSGLEQDFNSLDNQREACAAYIKS
                     QAHEGWRPVRERFDDGGFSGGSMNRPALQKLLDEVRGRKIDVIVVYKVDRLTRSLADF
                     AKLVELFDGHGVSFISVTQAFNTTTSMGRLTLNMLLSFAQFEREITGERIRDKVAASK
                     RKGIWMGGAVPLGYRVEDRALHVVEEDAVFVRGLFRRYLELGSVVRLKAALGAENVRS
                     PIRKCGSGRQMGGLSMLRGHLYWILSNPIYAGRLRHKGLIHEGLHEAIIDKETWDRVQ
                     ERLASQTPERRAPKPDEYSFLKGKLYDDRGHRMSPSHAAKGGRRWRYYVSRAALTGRK
                     QDAGSIASIPAAALEEAVTQAIRTHLGRWSIAADGEGASAAATEHPKLMNSRDATRLR
                     TDAWATNDIRDAIERVTVGTDQIEIQLSDAVVGEDRDRVLTIPWAPRSTRRRRAIIPR
                     AGDARPPIRAMRTKARGVLIEAMRNAHSWLDQLVRDTNLTIAALAAREGKSERSIRMT
                     LSLAFVAPQLARAAVDGRLPRGFSVERLTELPMLWDEQWRVLGLQAPAQGRSEVG"
     gene            complement(2085..2159)
                     /locus_tag="VHS97_14620"
     tRNA            complement(2085..2159)
                     /locus_tag="VHS97_14620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2125..2127),aa:Val,seq:cac)
     gene            2283..>2480
                     /gene="hisE"
                     /locus_tag="VHS97_14625"
     CDS             2283..>2480
                     /gene="hisE"
                     /locus_tag="VHS97_14625"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX3449487.1"
                     /translation="MGEKDRAGAMFTLDELAQTIDQRAGANAKASYTRSLLDKGAAHC
                     ARKFGEEAIELAIAAAALDENA"
BASE COUNT          503 a          774 c          803 g          400 t
ORIGIN      
        1 gtccaggatc gcgaaggcca tcacaggcac aacttggaac ggccatcatt tcttcgggct
       61 caagccagcg acaagcaaga gtcccgaggt aggcttgaaa aggctgcgtc ggattgttgt
      121 ggatccggag accgcgtcat gatgcggacc gcgcccaggg cgagcctcca ctgcgcggtc
      181 tacacccgcg tttcgaccga ctcgggcctc gagcaggatt ttaactcgct cgacaaccaa
      241 cgggaggcct gcgcggccta tatcaagagc caagctcacg aagggtggcg gccagtccga
      301 gagcgcttcg acgacggggg attctccggc gggtcaatga atcgacccgc cctacaaaag
      361 ctgctcgacg aggttcgcgg gcgaaagatc gacgtgatcg tcgtctacaa ggtcgatcgg
      421 ttgacccggt cgctggctga cttcgccaag ctggtcgaat tgttcgatgg ccatggggtc
      481 tccttcatct cggtgaccca ggccttcaac accacgacca gcatgggtcg gctgaccctt
      541 aacatgctgc tctccttcgc ccagttcgaa agggagatca ccggcgaacg catccgcgac
      601 aaggtcgccg cctcgaagcg caaaggcatc tggatgggag gagcggtccc gctcggctac
      661 cgggtggaag accgcgccct ccacgtcgtc gaggaggacg ccgtgttcgt ccgtggccta
      721 tttcgccgtt atctcgaact tggcagcgtg gtccggctca aggccgcgct cggagccgag
      781 aacgtccgct cgccaatccg gaaatgcggg agcggtcggc agatgggcgg tctctcgatg
      841 ttgcgcggcc atctctactg gatcttgtcg aacccgatct atgcgggacg gctgaggcac
      901 aagggactga tccacgaggg cctgcatgaa gcgatcatcg acaaggaaac ctgggaccgg
      961 gtgcaggaac gactggcgtc acagacgccc gagaggcggg cgcctaagcc ggacgaatat
     1021 tcgtttctca agggaaaact ttatgacgat cgcggccacc ggatgagtcc gagccatgcg
     1081 gccaagggcg ggcgccgctg gcgatactat gtgtcgcgcg cggctttgac gggtcgaaag
     1141 caggatgctg gatcgatcgc cagcattccg gcggcggccc tcgaagaagc cgtcacccag
     1201 gcgattcgga ctcacttggg gcgctggtcg atagcagcag acggcgaagg cgcatctgcg
     1261 gcggccaccg aacaccccaa gctcatgaat agcagagatg ctacccggtt gcgaacggac
     1321 gcgtgggcca cgaacgacat tcgggatgcg atcgagcgag tcacggtcgg gaccgatcag
     1381 atcgaaatcc aactgagcga cgctgtcgtt ggcgaggata gggaccgtgt tttgaccatc
     1441 ccgtgggctc cgcgttcgac ccgccgtcga agagcgatca ttccacgggc cggcgacgcc
     1501 cgaccgccga tcagggcgat gcggaccaag gcccgagggg tcctcatcga agctatgcgc
     1561 aacgcccact cctggctcga tcaacttgtt cgggacacga acctcacgat cgccgctctc
     1621 gccgcccgcg aaggcaaaag cgagcgttcg atccggatga ccctgtcgct tgcattcgtc
     1681 gctccccaac tggcgcgggc cgcagtcgat ggccgcctgc cgcgcggctt cagcgtcgag
     1741 cgactgacag aactgccgat gctctgggac gaacaatggc gggtcctggg gctacaagcg
     1801 cccgcccaag ggcgatccga ggtcggctga accaaaaaac gccgtcgcgt cccgggaccg
     1861 tgaattccga tcgcgcctac tttccgcgga accgtcaatc tcggccaaga gaccatcccg
     1921 ggaaacggaa ttttgccggc ggagactggc ggccggtttg gggtctctgg cgcgacaaaa
     1981 cgtgcccaac ccctgttcgc agaccaggac ggtgcctcgg aaacgcccgg taaacggcgg
     2041 aaaaactccc tcccgcgaag cttcgttagt ttcacaggac tgcctggtgg gcgatgcagg
     2101 gatcgaacct gcgacccctc ccgtgtgaag ggagtgctct accgctgagc taatcgccct
     2161 aagcctcaag gtcgggccat gctttatggc tcccgcgaga gccaagtcaa gaagcactcg
     2221 gcgcggcgaa cagccgccgg ccccctgttg cggcggccgg cgcggcggga gataaaggcg
     2281 cgatgggcga gaaggacagg gcaggcgcga tgttcacgct ggatgaactc gcgcagacga
     2341 tcgaccagcg cgccggggcg aacgccaagg cctcctatac ccgcagcctg ctggataagg
     2401 gcgcagccca ttgcgcccgc aaattcggcg aagaagcgat cgaattggcg atcgccgcag
     2461 cggcgctgga tgaaaacgcc
//
LOCUS       DATAQL010000842         6565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2379120, whole genome shotgun sequence.
ACCESSION   DATAQL010000842 DATAQL010000000
VERSION     DATAQL010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379120"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VHS97_14630"
     rRNA            complement(<1..413)
                     /locus_tag="VHS97_14630"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1127..2158)
                     /locus_tag="VHS97_14635"
     CDS             complement(1127..2158)
                     /locus_tag="VHS97_14635"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX3449488.1"
                     /translation="MKVGLLTGGGDCPGLNAVIRAVVQQLANGGASCVGILEGWRGLV
                     QNMTSPLTVAATDGIISQGGSILGASRTNPYRNPDSNLAALRENFARLDLSSLIVIGG
                     DDTLGVAIRLHNDFGIPVVGIPKTIDNDLAVTDFSFGFDTAVNIVTEAVDRLRTTAES
                     HRRIIVVETMGRHSGWIACFAGIATGADYILVPEVPIDLPHLIGVLSNRRAVGKKYGL
                     VIVAEGAKFPDQAPLTLGQHPDPFGNLRLGGIGGVIADAIAKQTGVETRSVALGHLQR
                     GGSPSAYDRILATRMGLAAGRLALEKRFGTIVTLTGGQIVESIMSQSSVATKTLDLAY
                     YNEATAFFH"
     gene            complement(2429..3796)
                     /gene="accC"
                     /locus_tag="VHS97_14640"
     CDS             complement(2429..3796)
                     /gene="accC"
                     /locus_tag="VHS97_14640"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX3449489.1"
                     /translation="MFQRILVANRGEIALRVIRACKELGIEVVAVYSQADRDAPYLSQ
                     ANRSICIGKASSSDSYLNRSRIIAAAEVADAQAIHPGYGFLSENPDFAEVCRSSGFEF
                     IGPPHEAIRKMGLKTEAKQIAAMAQVACVPGSDGVIATDAQALRLARTIGYPILIKAA
                     AGGGGKGMRVCRDESTLSAALQTARNEAEAAFKNSSVYLEKFIDRPRHVEVQILADTS
                     GNIVHCWDRDCSLQRRHQKLVEESPAPTLPLEVRARLGDAAVRLARAAGYVNAGTCEF
                     LVDAENNFYFIEVNARIQVEHPVTEMVTGIDLVKQQIKIASGLPLPFAQEDIVVRGHS
                     IECRINSEDPDHDFRPSPGRITALNVPGGPGVRWDSHIQVGYTVPPYYDSLLGKLIVC
                     APTRPEALATMRRALDELVIEGVSTTIPLQRLIFRNHDFISGRVDTTWVERVLLAPKT
                     ASRPT"
     gene            complement(3844..4356)
                     /gene="accB"
                     /locus_tag="VHS97_14645"
     CDS             complement(3844..4356)
                     /gene="accB"
                     /locus_tag="VHS97_14645"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX3449490.1"
                     /translation="MAENPSGPTKPLDVQRLHYLVRLMKRYDLTALDVSDGSVQIKLR
                     RRSPHGESTALKPSQATSLSAANQASSQLLAQPPVEAPAAAASPPPKTVVIESPMVGT
                     YYSSSAPDLPQFVSVGSVVQPNSIVCIIEAMKVFTDIPANVSGTIAEILVKNGQAVEY
                     GQPLFRVIPA"
     gene            complement(4474..5571)
                     /locus_tag="VHS97_14650"
     CDS             complement(4474..5571)
                     /locus_tag="VHS97_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX3449491.1"
                     /translation="MDRHHSRREKLRASLRSHELDAFLVAAPTNVSYLSGFSGDSSVL
                     ILGRERDFIVSDGRFTTQLAEECPGLEAHIRLPGQETNAAIAHLLGTLGYRGVGLEAT
                     TWTIAEYEAICALAKTVSFTGVKGWVEALRQIKDDDEISAIRQAIGYAERAFMMLRAG
                     LRAGESEKDVADLLECYLRRCGATAASFPPIVAVGTRSAMPHARPTTSTAIGQADFVL
                     VDWGATGRPYKSDLTRVLVTGKVTTTFEAVYRTVLTAQERGIAAIRPGAKAHYVDAEA
                     RSVIEDAGFGRFFDHGLGHGLGMDIHEAPRLRKESSVTLEPGMVVTVEPGVYLPDWGG
                     IRIEDDVLVTPEGCEVLSHIPKSLDSVRVET"
     gene            5756..6412
                     /locus_tag="VHS97_14655"
     CDS             5756..6412
                     /locus_tag="VHS97_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3449492.1"
                     /translation="MPTMMQGCLIGATLAVLAAAGAEKDFRVIFDGTSGTGWVLCDKK
                     PLPKENVQTDGLNPHGTGSYLVVYETKFSDFVLDFDYKLTKGCNSGVFIRVSDLSHPV
                     PTGIEVALDDTTGTGMHDSGAFYDLVAPKQNAQKPAGEWNHMTIAARGPNMTITLNAK
                     EVSMINLDEWNEPGKRPDGSSHKFDNVAIGKLPRTGYLGFQDHGSNCWFKNVKIKELD
                     "
BASE COUNT         1359 a         1994 c         1934 g         1278 t
ORIGIN      
        1 aggccttcat cctccacgcg gcgtcgctcg gtcaggcttg cgcccattgc cgaagattct
       61 cgactgcagc ctcccgtagg agtctgggca gtgtctcagt cccagtgtgc cgggccacgc
      121 tcgcacgccc ggtaggcatc agaggcttgg tgggccatta ccccgccaac aacctaatac
      181 cacgacggcc cgtcccacgg cgccgaaact ttggttcacg ccggatgccc ggcacgaaca
      241 tcgctgggaa ttacccgcag tttcccgcgg ctatgcccaa ccatggggtt ggttgccgtc
      301 gccttactca cccttacgcc actgacttga tctttcaacc aagcccgttc gacttgcatg
      361 cctaatccac gccgccaacg ttcattctga gccaggatca aacccttcac aaatgagtcg
      421 gtccttaccg aaacgccgcg aaattcgcgg ctttccgcca agttccgatc ctcaccccaa
      481 agggttgagg aaaaatgctg gtcgcacgtc gcttctcgtg gaccgaacga atttcccggt
      541 cggctttcgc tccaggcatg cacttacgag cctacccgac cgaaaaccat caggatatcc
      601 attcgtccca ctcgaaacgt gtgccggtcc ttgcgagcca tcccgccgct ttgtggcctg
      661 gggaccaccc agaccgggcg tttacaggat tgaccgctcg cacgccggct tccctcaagg
      721 gtcatttgac gcgacgaatc tatgcttatc tcgtctcgtc ttggctgacg aatgcaatcg
      781 accggattgt caaagaactg ccgctcaatc aaacgggagg tagtgccagg aatgctctag
      841 ccactgcgct cgacccaaag cggcattgcc tcgcctcatc caccgacacc atcggccggt
      901 gcaaagctgc atttgagcaa ctcgccccgg ccgtcgcatg gctttgattt tgtggcaaag
      961 cgaattacat agtaacacgg ttgggcgcca cgtcaaaccc ctagtgattt tttttgcgga
     1021 aaacggcgcc ggcaactatt gattatcatg tactgaacag ttatcagtac tgctgcgttg
     1081 atcgaccaag ctcgtgtttc tcgaccggcc acggcagctg ggaattttaa tggaagaacg
     1141 ccgtggcttc gttgtaataa gccaggtcca gggtcttagt cgctaccgag ctctggctca
     1201 tgatcgattc cacgatctgg ccgcccgtca acgtgacgat ggtaccgaag cgtttctcga
     1261 gcgccagccg gcccgcggcg aggcccatac gtgtggccag aatgcggtcg tacgcactgg
     1321 gcgaaccgcc gcgctgcagg tgccccaagg cgacagagcg cgtctcgacc ccggtctgct
     1381 tggcgatggc atcggcgata acgccgccga tgcctcctaa ccgaaggttg ccgaaaggat
     1441 cagggtgctg cccgagcgtc agcggagctt ggtcgggaaa tttggcccct tcggcgacga
     1501 tgaccaggcc gtacttcttg ccgacggcgc ggcggtttga caagaccccg atcaagtgcg
     1561 gcaaatcgat cggcacttcg ggcacgagga tgtaatcggc tccggtcgcg ataccggcga
     1621 agcaagcgat ccagcctgag tgtcgtccca tcgtctcgac cacgatgatc cgccggtggc
     1681 tttcggccgt ggtccgcagc cgatcgacgg cttcggtcac gatgttgacg gccgtgtcga
     1741 aaccaaatga aaaatcggtc accgccaaat cgttgtcgat ggttttggga atgccgacga
     1801 cggggatgcc aaagtcattg tgaagtcgaa ttgccacgcc cagcgtatcg tcgccgccaa
     1861 tgacaatgag gctactcaag tccagacgcg cgaagttttc gcgcagagcg gccaggttgc
     1921 tgtcggggtt gcgataagga ttggtccgtg aagccccgag aatactccct ccctgcgaga
     1981 tgatcccgtc agtcgcggcg acggtcaggg ggctggtcat gttctgaacc aagccgcgcc
     2041 acccttcgag gatgcccaca cacgaggcac ctccgttggc gagctgttgc acgacggcac
     2101 ggatcaccgc attcaagccc gggcagtcac cgccgccggt gagcaaaccg accttcatgg
     2161 aatccatccc tttttgtgaa cgtgagagcg aactctcgag gaaaccgtgg caacgattgg
     2221 tccgttcaga gccaattcgt cgcgacgcgc acgacctctg gctctgagat tatcatggcg
     2281 catgaatcgc ggccagagcg agcacggccg atcaggctcg accgacctcg attaagtttc
     2341 cacaaccaag gattgcgccg ttttttgact acgaccaagc gggcacgcca agcgacatcg
     2401 gcgtgatttc tcatccagcg ctgctgattc aggtcggacg ggaggcggtc ttgggcgcca
     2461 gcaggacgcg ttcgacccag gtggtgtcga cccgcccgga tataaagtcg tggttgcgaa
     2521 agatcagccg ttgcaagggg attgtcgtgc tgacaccctc aataaccagt tcgtccaagg
     2581 cacggcgcat cgtggcgagg gcctccggcc gcgtgggggc gcaaacaata agcttgccca
     2641 agagtgagtc gtagtaaggg ggaaccgtgt acccgacctg gatgtgcgag tcccagcgaa
     2701 cgccgggccc ccctggtaca ttcagcgccg tgatccgtcc cggcgatgga cgaaaatcgt
     2761 ggtcaggatc ttcggaattg atccggcatt cgatcgagtg tccgcggacg acgatgtctt
     2821 cttgagcgaa cggcaggggg aggccggagg cgatcttgat ttgctgtttg accaggtcga
     2881 tgccggtgac catctcggta acggggtgtt cgacctggat gcgcgcgttg acctcgatga
     2941 aataaaagtt gttctcggcg tcgaccaaaa actcacacgt acccgcgttg acgtagccgg
     3001 cggctcgggc caaccggact gcggcgtcgc ccagacgggc tcggacctcc aggggcaagg
     3061 ttggcgccgg agattcctca acgagctttt gatgacggcg ctgcaaagaa caatcgcggt
     3121 cccagcagtg aacgatgttg ccacttgtat ccgcgagaat ctggacttcg acgtgacggg
     3181 ggcgatcaat gaatttttcg agatagacgc ttgagttctt gaacgcggcc tcggcctcgt
     3241 tccgagcggt ctgaagcgcg gccgacagtg ttgattcatc acggcagacg cgcatcccct
     3301 ttccgccccc tccggctgcg gccttgatca agatgggata gccgatcgtg cgggccaagc
     3361 gtagcgcctg cgcgtcggtg gcaatcacgc catcggaacc aggaacgcat gcgacctgcg
     3421 ccatggcggc aatttgcttg gcctcggtct ttaaccccat cttgcggatg gcctcgtggg
     3481 gtggcccgat gaactcgaaa ccgcttgagc ggcagacttc ggcgaaatcg gggttctcgc
     3541 tcaagaaacc gtaacctggg tggatcgcct gagcgtcggc aacctcagcg gcggcgatga
     3601 tccgagagcg gttcaagtag ctgtcgctgc ttgaggcttt accgatacag atggatcggt
     3661 tggcctggga caggtagggg gcgtcgcggt cagcctgtga gtagaccgca acgacttcaa
     3721 tacccagctc cttgcatgcg cgaatgacgc ggagggcgat ttcgccgcga ttggccacca
     3781 ggatccgttg aaacatcggc aggcgtcacc tcagttaaac cgaactgtcc gagcactccg
     3841 agctcaggcg ggaatgacgc ggaagagcgg ttgaccgtat tcaactgcct ggccattctt
     3901 gaccaggatc tcggcgatcg ttcccgagac gttcgccggg atgtcggtaa agaccttcat
     3961 cgcctcgatg atgcagacga tcgagttagg ctgaaccacg gagcccaccg agacaaactg
     4021 cggcaagtct ggagcgcttg aagagtagta ggtaccgacc atggggcttt cgatgacgac
     4081 ggtcttgggg ggcggcgagg cggccgctgc cggtgcttcc acgggaggtt gggcgagcag
     4141 ttgacttgag gcttgattcg cggcgctgag agacgtcgcc tgcgacggct tgagagcggt
     4201 tgactcgccg tgtggcgacc tgcggcgcag tttgatctgc accgacccat cgctgacgtc
     4261 aagcgcggtc aaatcgtagc gcttcatcag gcgaacgagg tagtggagcc tctggacatc
     4321 gagcggcttg gtcggaccgg aggggttctc cgccattcct tcactccgca attgcaactc
     4381 aaccgctccg cgccactctg ggtctcgcag tcaaaccgat cgtcacgccg agcggtggac
     4441 tgccataatc gagtgcgtgg cgacggcaga cacttatgtt tcgacacgca ccgagtcgag
     4501 cgacttggga atgtgtgaca ggacctcgca accttcgggg gtcaccagca catcgtcctc
     4561 gatccggatg ccgccccagt cgggcaaata cactccgggc tcgaccgtca ccaccatgcc
     4621 cggctcgagc gttacactcg actccttgcg gagccgtggg gcctcatgga tgtccatgcc
     4681 taacccatga cccagcccat ggtcaaaaaa acggccgaaa cctgcatcct cgatgacgga
     4741 gcgggcttcg gcgtccacgt aatgggcctt tgcgcccggc cggatcgcgg caattccgcg
     4801 ttcctgagcc gtcaggacag ttcgataaac cgcctcgaac gtggttgtga ccttacctgt
     4861 gacgagaact cgcgtcaagt cacttttata aggacgacca gtggcgcccc agtcgaccaa
     4921 gacaaaatcg gcctgaccga tcgcagttga cgtcgtggga cgggcatgcg gcatggccga
     4981 tcgagttccg actgcgacga ttggtggaaa actcgccgcg gtggcaccac agcgccgcag
     5041 gtagcactca agcaagtcgg caacatcctt ttcgctttcg ccggcacgca aaccggctcg
     5101 cagcatcatg aacgctcgtt cggcgtagcc aatcgcctgg cgaatcgccg agatttcatc
     5161 gtcatccttg atctgccgca atgcttcgac ccatcccttg actccggtga acgacaccgt
     5221 cttggctaga gcacagatcg cctcatattc ggcgatggtc cacgtcgttg cctcaagccc
     5281 cacgccacga taaccaagag tccccaagag gtgagcgatc gccgcgttcg tctcctgtcc
     5341 gggcagccga atgtgggcct ccagtcccgg gcattcttcg gcaagctgcg tggtaaaacg
     5401 gccgtccgaa acgataaagt cccgctcgcg accgagaatt agcaccgatg aatcaccgct
     5461 aaacccgctc aggtagctca cgttcgtggg cgcagcaacc aagaacgcgt cgagctcgtg
     5521 cgaacggagc gacgccctca gcttttcgcg gcgagagtgg tgtcggtcca tgggctcgct
     5581 cgtttggttt ccggaatgcc gctgtggtag cccagccaac aggctgattc ttgaagaatt
     5641 ccatggtagg gtaacgtgaa gctggcccgc cgcccagagg ctctggaatc ctgcccagcg
     5701 aaactaaact tcgagacgcg gtgactctcg atcgagttta ataaggagac gaccgatgcc
     5761 aactatgatg cagggctgcc tgatcggcgc gacgcttgcg gtgctggcgg ccgccggggc
     5821 ggaaaaggat ttcagagtta tcttcgacgg gaccagcggc acgggctggg tcctctgcga
     5881 caagaagccg ctgcccaagg aaaacgtcca gacggacggt ttgaatccgc acggcaccgg
     5941 gagttatctc gttgtttacg aaacaaaatt tagcgacttc gttctcgact tcgattacaa
     6001 gctgaccaaa ggctgtaact cgggcgtctt tatccgcgtc agcgacttga gccaccctgt
     6061 ccccaccgga attgaagtcg cgctcgacga cacgacaggg accggcatgc acgactctgg
     6121 tgctttctat gacctggtgg cgcccaagca gaacgcgcaa aagccagccg gcgagtggaa
     6181 ccacatgacc atcgccgccc gggggccaaa tatgacgata acgctcaacg ctaaggaagt
     6241 ctcaatgatc aatctcgacg aatggaacga gcccggaaag cgacccgatg gatcgagcca
     6301 caagttcgac aacgttgcca tcggcaaatt gccacgcacg ggctacctgg gtttccagga
     6361 tcacgggagc aactgctggt tcaagaatgt gaagatcaaa gagctggact gagctaaaac
     6421 aaccaccccg gacccgtcgg ttaccgcgcg ggtggggtgg tcggtttttt ttctgaatga
     6481 ctgctatcgt caaaggcgag cgaaattacc agtcgccacc accaccgcct ccccaatcgc
     6541 caccaccccc gccgcctccc caatc
//
LOCUS       DATAQL010000843         6277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5430926, whole genome shotgun sequence.
ACCESSION   DATAQL010000843 DATAQL010000000
VERSION     DATAQL010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5430926"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VHS97_14660"
     CDS             <1..901
                     /locus_tag="VHS97_14660"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3449493.1"
                     /translation="KGLGAMLLVHAESSRVLDELIARHHTPEMMSRYGARLHAMTRPD
                     FIEAEAIQRAITWAEVTGGRLYIVHMSTSQGADLVKAAQARGVDVYAETCAQYLVLDD
                     SMFDRADGHLFACCPQIKKKSDQERLWKGLNDGEVSVISTDTCTFSRAQKARWEGDWT
                     KIPMGLPGLETLLPIVFTHGVLAGRLTPAEFVAKCCTNPARLMGLYPKKGVIAAGSDA
                     DLAIIDPNTRIEVDCAQMETNADWSPYQGWSLAGFAETTFCRGRKIVADYKFVGESGW
                     GRWLPRERAGSLADRRRSSAISR"
     gene            979..2319
                     /locus_tag="VHS97_14665"
     CDS             979..2319
                     /locus_tag="VHS97_14665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3449494.1"
                     /translation="MFRAGEKQSLDVLKARFADVPPPLAVDEARIEAARCLFCYDAPC
                     TRACPTEIDVPRFIRQILHRDELGAARTILEANIMGGSCARACPTEVLCEGACVDRAL
                     AKAPVQIGRLQRFACDIASAREVRFFEPGLPTGKRVAVIGSGPAGLSCAHELRRLGHD
                     VVVFEARNLPGGLDTLGIAAYKISTEFALSEVEMIRQIGIAIELNHRVTGDEVRALLH
                     SFDAVFLGIGLGRTLPLGVAGEELEGVWEALDFIFQTHTKPLSECVVGLNVLVIGAGN
                     TAIDVATAARRLGSDSVTIAYRRSEASIPAFAYEYALARSDGVQFEWFCQPIRILGAG
                     GAATGVEFMRTELTSPDSRSGRVNTVLGSNFVIAADMVVKALGQEPLYDLVAALPDLK
                     TDGGKIVVDRSTRATSVAGLFAGGDCLRHGGEVVDAVQDGKMAARGIHAALQVT"
     gene            2344..3723
                     /gene="preA"
                     /locus_tag="VHS97_14670"
     CDS             2344..3723
                     /gene="preA"
                     /locus_tag="VHS97_14670"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEX3449495.1"
                     /translation="MPDLSIEFCGVKAPNPFWLASGPPTNSEYQVRRAFDAGWGGAVW
                     KTLADLPIVNVSSRYGAIDYDGQKVMGLNNIELITDRPLDVNLEEIMRVKKAYPDRAL
                     FVSLMVESKRDSWHEITKKTQNAGADGIELNFGCPHGMSERGMGSAVGQVPEYACMIT
                     EWVKEVATIPVMVKLTPNVTDVAAIGRAAVNGGADALSLINTINSIMGVDLDTLAPKP
                     SVRGFGSHGGYCGPAVKPIALNMVATVAADQEIKVPISGIGGIQAWQDAVEFLLLGAC
                     SMQVCTAVMHYGFRIVEHLQSGLEGWMREKCYRSIKELVGLSVPRIKPWGELDLNYKI
                     VAGIDQDKCIHCGLCYIACEDGCHQSIPVDRVKSTDYLASRGSQEGVFRSGGQIIIPG
                     AGGEAINMFSIKEDMCVGCNMCSLVCPVEGCITMREVDSGQPPLSWNEYQAKLARGEV
                     ATIAPPQHV"
     gene            complement(3746..4507)
                     /locus_tag="VHS97_14675"
     CDS             complement(3746..4507)
                     /locus_tag="VHS97_14675"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3449496.1"
                     /translation="MPVHDWTRVEAGIFHDFHTVWTGALRNALNEGLLPQGYYAMAEQ
                     HAGRTIADVLTLHASPEPPERAWLPPDTGGIAVAEAPPRVWRKQTVEPAALGRRRSLA
                     IRHVSGHRLVALIEITSPANKDRSRHVDELAGKAVSALEQGVHVLLIDLFPPGTHDPS
                     GIHGSILECLEQFGEPYVLPTDMPLTLASYVAGLRVEIYLEHLAVGAALKEMPLFVRP
                     DRYINVPLEATYQSTYRGMPAFWRDVLEGRSSGPS"
     gene            complement(4622..6010)
                     /locus_tag="VHS97_14680"
     CDS             complement(4622..6010)
                     /locus_tag="VHS97_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3449497.1"
                     /translation="MITNPSKRATKAPASLPISSHTPAPYDGPSRDEVLAMRRQYLTP
                     GLITYYREPLLIAEGHMQYVWDETGKQYLDAFAGIVTVSVGHCHPKIVEKVRAQVGRL
                     QHTTTIYLHPAVAQLGKKLAEHMPSESGLSISYFTNSGSEANEIAILSAREYTGNTEV
                     ISLRNGYHGGTQAAMALTAHGTWKFKSSPTMGVKYATPGYCYRCPYGLEYPSCDIKCA
                     RDVEDLIKYETSGEVACFIAEPIQGVGGAVTPPREYFQIVYDIVRKHGGLCVADEVQT
                     GFGRTGTAFWGFENWGVTPDLVTMAKGIGNGIPLGACVTRPEIASVMKNRIHFNTFGG
                     NPISMTQGLATLEVIDSEGIQQNALTIGGHLKERLLGLQERQPLIGEVRGMGLMLGVE
                     LVRDQQTKEPATAETADLVELCKDRGLLIGKGGLAGNILRIKPPMCLTRDDADFLVDC
                     LDEVLTMISGRV"
     gene            complement(6058..>6277)
                     /locus_tag="VHS97_14685"
     CDS             complement(6058..>6277)
                     /locus_tag="VHS97_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX3449498.1"
                     /translation="NRPGFEEPWRIGEFYGQSYFCDPRGQMLAVGKRDSDDIVIADLN
                     LDVIREVRNTWQFFRDRRPETYGSIVEL"
BASE COUNT         1182 a         1903 c         1969 g         1223 t
ORIGIN      
        1 caaggggctc ggcgcgatgc tcctggtgca cgccgagtcg agccgcgtcc tcgacgagct
       61 gatcgcccgc caccacaccc ccgagatgat gtcccgctac ggcgcccggc tccacgccat
      121 gacccggccc gacttcatcg aggccgaggc gatccagcgc gccatcacct gggccgaggt
      181 tacgggcggc cgcctgtaca tcgtccacat gtcgacatct cagggagccg acctcgtcaa
      241 ggctgcccag gcgcgtggcg tcgacgtcta cgccgagacc tgcgcacaat acctggtact
      301 cgatgactcg atgttcgacc gggctgatgg ccatttgttt gcctgctgcc cccaaatcaa
      361 gaaaaagagc gaccaggaac gtctgtggaa agggctgaat gacggtgaag tgtcggtaat
      421 ctcaaccgat acctgcacgt tcagccgagc ccagaaggca cggtgggagg gagattggac
      481 caagatcccg atggggttgc ccgggctcga aacacttttg ccgatcgttt tcacgcacgg
      541 agtgctggca ggccggctca cgccggcgga gtttgtcgcc aagtgctgca ccaacccagc
      601 caggttgatg ggtctttatc ccaagaaagg ggtaatcgcc gcggggagcg acgccgacct
      661 cgcgatcatc gatcccaaca cgcgaatcga agtggattgc gcccagatgg aaacgaacgc
      721 cgactggagc ccctaccagg gctggtcgct ggcgggtttt gccgagacca cgttttgccg
      781 tggacgcaag atcgtcgcgg attacaagtt tgtaggcgag agcggctggg gccgctggct
      841 gccgcgcgag cgagcgggat cgctcgccga ccggcgacgg tcgagcgcga tctcccgctg
      901 agtccggcaa acccgggccc ttgcgcttct ggaacccaac tcggcgggga aggctcggac
      961 cgagccaagg agtgactcat gtttcgggcc ggtgagaagc aatcgctcga cgtcttgaaa
     1021 gcgcggttcg cggacgttcc accacctttg gcagttgacg aagcccggat cgaagcggcg
     1081 cgctgcttgt tttgctacga cgcgccgtgc accagggcct gcccaaccga gatcgacgtc
     1141 ccgcgcttta tccgccagat cctccaccgc gatgagctcg gcgcagccag aacaatcctt
     1201 gaggcgaaca tcatgggggg cagttgcgcc cgtgcctgtc caaccgaagt cctttgcgag
     1261 ggggcctgcg tcgatcgcgc gctcgcgaag gctcccgttc agatcggccg cctgcaacga
     1321 tttgcctgcg acatcgcgtc ggcccgagag gttcggttct tcgagccggg cttgccgacg
     1381 ggcaaacgag ttgccgttat cgggtcggga ccagctgggc tttcctgtgc acacgaactg
     1441 cggcggctgg ggcacgatgt cgtcgtcttt gaggcgcgga acctgccggg aggccttgat
     1501 acactgggga tcgccgccta caagatctca accgaattcg ctctctctga agttgagatg
     1561 atccgccaga tcggcatcgc aatcgagctc aaccatcgtg taactggcga cgaggttcgc
     1621 gcactgctcc actcgttcga cgcggtattt ctgggcatcg gtttgggacg cacgctgcct
     1681 ctgggggtcg ccggtgagga gcttgagggc gtttgggagg cgctcgactt catttttcag
     1741 acccacacca aacccctcag cgagtgcgtg gtcggcctta atgtgcttgt gatcggcgcc
     1801 gggaacacgg cgatcgacgt cgccaccgcc gccaggcgac tcgggtccga ctccgtgacg
     1861 atcgcctatc ggcgctcgga agcttctata ccagccttcg cctacgaata cgccctggcg
     1921 cggtccgatg gagtccagtt cgaatggttt tgtcagccta tccgcatctt gggcgccggc
     1981 ggcgcggcca cgggtgtcga attcatgcgg acggagctca catcaccgga ttcgcggtcg
     2041 ggacgcgtga atacggttct tggctcgaat tttgtcatcg ccgccgacat ggtggtcaag
     2101 gccctcggcc aggagccgct ctacgacctc gtcgctgcct tgcccgattt gaagaccgac
     2161 ggcggcaaga tcgttgtcga tcgatccacc agggccacca gcgtcgccgg cctcttcgcc
     2221 ggcggcgatt gtctgcgcca tggcggcgag gtcgtcgatg cggttcagga cggcaagatg
     2281 gccgcgcggg gaatccacgc cgcgctgcag gtgacgtgac ttaaccacgg tgcaggagtc
     2341 ctcatgcccg acctgtcgat cgaattctgt ggggtcaagg cgcccaatcc cttctggctc
     2401 gcgtccggtc cgccgaccaa ttccgaatac caagttcgcc gcgcgttcga cgccggctgg
     2461 ggaggagcgg tctggaagac cctcgccgac cttccaatcg tcaatgtgtc gtcgcgctac
     2521 ggggcgatcg actatgacgg ccaaaaggta atgggactca ataatattga attgatcact
     2581 gaccgtccgc tggatgtcaa cctcgaggag atcatgcggg tcaagaaggc gtatccggat
     2641 cgggccctgt ttgtgtcgct catggtcgaa tcgaaacgag actcgtggca cgagatcacc
     2701 aagaagaccc agaatgctgg cgccgacggc atcgagctca atttcggctg cccgcacggc
     2761 atgagcgagc gcggcatggg ctcggcggtt ggacaggtgc ctgagtacgc gtgcatgatc
     2821 accgagtggg tcaaggaagt cgcgacgatc cccgtcatgg ttaagctgac gcccaacgtc
     2881 accgatgtgg cggcaatcgg ccgcgccgcc gttaatggcg gggccgatgc actctcgctc
     2941 atcaacacga tcaactcgat catgggtgtg gacctggaca cgctcgcgcc gaagccgtcg
     3001 gtccgcggct ttggcagcca cggcggttac tgcgggcccg ccgtcaagcc gatcgcgctc
     3061 aacatggtgg ccacggtcgc ggccgatcaa gagatcaagg tcccgatcag cggaattggc
     3121 ggcatccagg cctggcaaga tgccgtcgag ttcctcctct tgggcgcctg ttcgatgcag
     3181 gtttgcacgg ccgtgatgca ctacggcttc cggatcgtcg aacatttgca gagcggcctg
     3241 gaaggctgga tgcgtgaaaa gtgctaccgg tcgatcaagg agctcgtcgg gctttccgta
     3301 cctcggatca agccgtgggg cgaactcgac ttgaactaca agatcgtcgc cgggatcgac
     3361 caggataagt gcattcactg cggactctgt tacatcgcct gcgaggacgg ctgccaccaa
     3421 tcgattcccg tggaccgtgt aaagtcgacc gactacctcg cgtcgcgtgg ctcgcaagaa
     3481 ggggtgttcc gctcgggcgg ccagatcatc atcccgggcg cgggcgggga agcgatcaac
     3541 atgttttcaa tcaaagaaga catgtgcgtc ggttgcaata tgtgcagtct ggtctgcccg
     3601 gtcgaaggct gcatcacgat gcgggaagtc gactcgggtc agccgccctt gagctggaac
     3661 gaatatcagg ccaaattggc acggggcgaa gtcgctacga tagcgccgcc gcagcacgtt
     3721 tgaggaaccg cggatcccgc cgttgtcagg acgggccgga gctgcgtcct tcgagcacgt
     3781 cacgccagaa ggcgggcatg ccgcggtagg tggactgata agtcgcttcg agcggcacgt
     3841 taatataccg gtcgggccgc acgaatagcg gcatctcttt caaggcagct ccaacggcaa
     3901 gatgctcgag atagatttca acccgcaagc ccgcgacgta gctggccaac gtcaacggca
     3961 tgtccgtcgg tagcacgtac ggttcgccga actgttccag gcactcgagg atggatccat
     4021 ggatcccgga agggtcgtgc gttccgggtg gaaaaaggtc aatcaaaaga acgtggacac
     4081 cttgctcaag cgccgagacg gccttgccgg ccagctcatc gacgtgccgg ctacgatcct
     4141 tgttcgccgg tgaggtgatc tcgatcaaag cgaccaggcg atgaccgctg acgtgacgaa
     4201 tcgccaggga gcgccgtcga cccaacgcgg ccggctccac ggtctgcttg cgccagactc
     4261 ggggtggcgc ttcggccaca gcgatgccgc cggtgtctgg gggcaaccac gctcgctccg
     4321 gaggctctgg gctcgcgtgg agcgtgagca cgtccgcgat cgtgcggcca gcgtgctgct
     4381 ccgccatcgc gtaatacccc tgtggcaaca gtccttcgtt cagcgcgttc cgaagcgcgc
     4441 cagtccagac tgtatgaaag tcatgaaaga tgccggcctc aacgcgtgtc cagtcgtgga
     4501 cgggcatggt tcagtctcct ccggatcgtg caaccgtatt gtaccccagg cggtcggcgg
     4561 gcgagaacag aaagacatgc ggatactcgc gcttcgtcgc gtcaacgttg acgaacagtt
     4621 attacactcg acctgaaatc attgtcaaaa cttcgtccaa gcaatcgacc agaaaatccg
     4681 catcgtcgcg ggtcaggcac atcgggggct tgatccggag tatgttgccg gccagacctc
     4741 ccttgccgat cagcaggccc cggtccttgc aaagctcgac aaggtcagcg gtctcggccg
     4801 tggcgggttc cttcgtctgc tggtcgcgca ctaattccac accaagcatc aggcccatgc
     4861 cgcgcacttc tccgatcaat ggctgacgct cttgcaggcc gagcagtctt tccttgagat
     4921 gcccgccgat cgtcaaggcg ttttgttgga tcccctcgct atcgatcacc tcgagtgtcg
     4981 ccagaccttg ggtcatcgaa atcgggttgc cgccgaaggt gttgaaatgg atacggtttt
     5041 tcatgacgct cgcaatttcc ggccgcgtga cacaggcgcc tagcggtata ccattaccga
     5101 ttcccttggc catggtgacg aggtccggag taacgcccca gttctcgaaa ccccagaacg
     5161 ccgtcccggt ccggccgaac ccggtctgga cctcatcggc cacacagagc cccccatgtt
     5221 tgcggacaat gtcgtagacg atctgaaagt actcgcgcgg cggcgtgact gcaccgccaa
     5281 ctccctggat gggctcggcg ataaagcagg cgacctcgcc tgatgtctcg tacttgatca
     5341 ggtcttcgac gtcgcgcgca cacttgatgt cacaactcgg atattccagt ccataggggc
     5401 aacgatagca gtaacctggc gttgcatact tcacgcccat cgtcgggctt gatttgaatt
     5461 tccaggtccc atgcgcggtc agggccatgg cggcctgtgt gccaccgtgg taaccgttgc
     5521 gcaggctgat aacttcggta ttgcctgtgt attcgcgagc cgacaggatc gcgatttcgt
     5581 tggcctcact gcccgaattg gtgaaatagc tgattgacag gccactttct gaaggcatgt
     5641 gctcggcgag ctttttgcca agctgggcaa ctgcgggatg caaatagatc gtcgtcgtgt
     5701 gttggagccg gccaacctgc gcgcggactt tctcgacaat cttggggtga cagtggccga
     5761 cggacacggt gacgattcca gcgaacgcgt cgaggtattg tttgccggtt tcgtcccaga
     5821 cgtattgcat gtggccctcg gcgatcagca agggctcgcg gtaatacgtg atcagccccg
     5881 gcgtaaggta ctgccggcgc atggccagca cttcgtcgcg cgaagggccg tcatatggag
     5941 ccggtgtgtg cgaggagatc ggcagcgacg ccggggcctt ggttgctctc tttgacggat
     6001 tggtgatcat ttcagtttct tcgctccagg gaggctgggc cgcgacgtgg gtgcggctta
     6061 gagctcgacg atcgacccat aggtctcggg ccgacgatcg cggaaaaact gccatgtatt
     6121 gcggacttcg cggatcacgt ccagattaag gtcggcgatc acgatgtcat cgctgtcgcg
     6181 tttgccaacg gcgagcatct gtccgcgagg atcgcagaaa tagctctgtc cgtagaactc
     6241 gccgatccgc cacggctctt cgaatccagg gcgattg
//
LOCUS       DATAQL010000844         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1768162, whole genome shotgun sequence.
ACCESSION   DATAQL010000844 DATAQL010000000
VERSION     DATAQL010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768162"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VHS97_14690"
     CDS             <1..1068
                     /locus_tag="VHS97_14690"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX3449499.1"
                     /translation="LAAGVSTHRAIRPVLVSSVIVSTFAIANQELVMPPNAEELAKSH
                     DDDGERTVHVSSRYDSRSLQISGADADRASKTVLMIDVVIPVGVFGEMKLLRGKQGTF
                     IPPDHPTAPLKAGWLIRNGTINPPLDGEALKTSRELITLVDDLTGFPPPYAPPAAKAD
                     GQAPPGDRQPPPAATRPPDNPFRPHSEIPYLASLPPLPLCLNPVILKVYSLFDRKVDL
                     SRGNYFLKSSLTFQAMTRKPDWYKFATTRDLIEGLSDPSTEGTERTAVAIFVHGRILR
                     PILGLNLLFMSLPLVLGGYGRNTFINLGLALGNTAIFYGAILFCQYLGSFEFFSPPMS
                     AWAPLMLFGAIATIRWGQIRS"
     gene            complement(1125..2774)
                     /locus_tag="VHS97_14695"
     CDS             complement(1125..2774)
                     /locus_tag="VHS97_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX3449500.1"
                     /translation="MSLIRETVGHIRDLPRYRQILTSLVRYGYQDVVAALHLEGIVRP
                     IERVALGNDVPPQDRPRRLRLVCEDLGPTFVKLGQLLSTRPDLLPETYTNELAALRDD
                     VRPFSFAQVATILKEEYGRPLDELFASVEEIPVASASISQVHRAVLPNGRIVALKVRR
                     PEIAKVIQADLDIIKNLAQLVERRLPNLAVYRPLSLAREFERTIKRELDFSTERRTMQ
                     RCRIQLGGDPTAHIPAVFDELSTSSVIAMEFIDGVSIDDLDGIRRLQLDPAKVAVTGA
                     RILLRQIFEFGFFHADPHPGNLRVLEGGVIAPLDFGMFGLLDGKTRERIAGLLSGLLA
                     QDADRVIRALESLDIRGLQLDSRGFRRDIAELVASYSELTLETIDLSVLLQELVAVIR
                     THHLQIPADLVLLIRSLVTIESVGRKLNPHFDIAGELVPFIRDLTLRRFRPDRILSQT
                     VRTSEDLQRIATLLPDLLSQSLDSIKRGELRVQFDLQGFERMVAKLARASNSLTVGIV
                     IAGLLVASSLVLRAGSVTVAYAGFASGLVLCLWLVWNMTHD"
     gene            2979..3308
                     /locus_tag="VHS97_14700"
     CDS             2979..3308
                     /locus_tag="VHS97_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449501.1"
                     /translation="MPIQQQSAPVRQLESDEEFFETRLEAIGFIFHGTIADANGKAHT
                     SRSSSNLLHFARCAKLEKVGDTETKIWFRSVRVAIKHLDELIGHPRWKWCKVCEREIT
                     QRILDET"
     gene            complement(3450..3971)
                     /locus_tag="VHS97_14705"
     CDS             complement(3450..3971)
                     /locus_tag="VHS97_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449502.1"
                     /translation="MTPNSEDQIRKQLDEAEGYLMLDLPERSLQILESRPDWLSMQFE
                     ASFLKGEALRNLKHYREALRPLEVAAGLRPSDTRVALALGWCYKRTNRLAQAIDSLER
                     ALREHPDEALLHYNLACYWSLARNSSKALDALTSALALDAELRTMIADEADFNHLRGN
                     PEFERLMGHAPSA"
     gene            complement(4191..>4529)
                     /locus_tag="VHS97_14710"
     CDS             complement(4191..>4529)
                     /locus_tag="VHS97_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449503.1"
                     /translation="HLFHYFVPEDAKNTLIVTFGSFQSRWPLFAHIGQHVGWFVRHKI
                     RTTVDEDALILENLADDSTHLDGMKLSRFDRVLGLTRERIQRIYYGDTEAQKPTPHAG
                     TLAATSGVTS"
BASE COUNT          936 a         1338 c         1351 g          904 t
ORIGIN      
        1 ctagcggccg gggtcagcac ccatcgcgcc atccgcccgg tactggtctc gtccgtgatc
       61 gtgagcactt ttgcgattgc caaccaagag ctggtcatgc ccccgaatgc cgaggaactg
      121 gcaaaatcac acgacgacga cggcgaacgc acggttcacg tctcgagccg ttatgattct
      181 cggtctttgc agatctccgg ggcggatgcc gatcgggcat cgaagacagt tctgatgatt
      241 gacgtcgtaa tcccggtcgg cgtctttggc gagatgaagc tgctaagagg caagcaagga
      301 actttcatac cgccggatca tcccaccgct ccgctcaaag cgggctggct gatccgcaac
      361 gggacgatca acccgccgct tgatggcgag gctctgaaaa cgagccgtga gctcatcacg
      421 ctggtcgacg acctgaccgg attcccaccg ccgtacgccc ctccagccgc caaggcggac
      481 ggtcaggccc cccccggtga cagacagcca cctcccgccg cgacgcgccc accggacaat
      541 cccttccgtc cccactcaga aatcccctat ctggcttcgc tgccgccgct gccgttgtgc
      601 ctcaatccag taattctcaa ggtttacagt ctgtttgacc gcaaggtcga tttgagccgg
      661 ggcaattatt ttctgaaatc aagtctgacg tttcaggcga tgacgcgcaa gccggattgg
      721 tataaatttg cgaccacgcg tgacctgatc gaaggtcttt ccgacccgtc tactgagggg
      781 accgaacgaa ccgccgtggc gatcttcgtc cacgggcgaa tcctgcgccc gattctcggt
      841 ttgaatctac ttttcatgag cctgcctctg gtgctcggcg ggtacggacg caacacgttc
      901 atcaaccttg gcctggcact gggaaacacg gcgatctttt acggcgcgat tcttttttgc
      961 cagtatctcg gtagtttcga gttcttttca cccccgatgt ccgcctgggc tccgctcatg
     1021 ctgttcggag ccatcgcgac catccgctgg gggcagatcc gcagctgacc cgctggcacg
     1081 ccacgcgaca aagtcgccgt gcatcgcgac aaagtcgccg tgcatcagtc gtgcgtcata
     1141 ttccagacca gccagaggca aagcaccagg ccgctggcga agccggcata ggcgaccgtc
     1201 acggagccag cgcgcagcac cagggacgag gccacgagca aacccgcgat cacaatgccg
     1261 accgtcaggc tattgctggc ccgggccagc ttggcgacca tccgctcgaa accttgaaga
     1321 tcgaattgaa cgcgcagttc gcctcgtttg atcgaatcta gggactgact caaaagatcg
     1381 gggagcaacg tggcgatgcg ctggaggtct tcgcttgtcc ggacggtctg gctcaatatc
     1441 cggtcagggc gaaaacggcg aagcgtgaga tcgcggatga acggcacgag ctcaccggcg
     1501 atgtcgaaat gcgggttcag tttacggccg acgctttcga tggtcacgag cgagcggatc
     1561 aagagcacca ggtcggcggg aatttgaagg tggtgggtcc ggattacagc cacgagctct
     1621 tggagcagta ccgacaggtc gatcgtctcg agagtgagct cggagtacga cgccaccagt
     1681 tcggcgatgt cgcggcgaaa accgcgcgag tcgagctgaa gtcccctgat gtcgagcgat
     1741 tcgagtgcac ggatcacccg gtccgcgtcc tgagcgagca gaccgctcaa gaggccggcg
     1801 attcgctcgc gggttttgcc atcgagcagt ccgaacatcc cgaaatcgag cggggcgatc
     1861 acccccccct ccaggacgcg caggttaccg ggatggggat ccgcgtggaa aaacccgaat
     1921 tcgaaaatct gcctgagcag aatccgcgcg ccggtgacgg cgactttcgc cgggtcgagt
     1981 tgcaagcgac gaattccgtc cagatcgtcg atcgaaactc cgtcgataaa ctccatcgcg
     2041 atcacgctgg atgtcgagag ctcgtcgaaa accgctggga tgtgtgccgt cggatcgccg
     2101 ccgagctgga ttcgacaccg ttgcatggtc ctccgctcgg tagaaaagtc gagctcgcgc
     2161 ttgatcgtgc gctcaaattc gcgcgccaga gagagcggcc gatagacggc caggttgggc
     2221 agacgccgtt ccacgagttg ggccaagttt ttgatgatgt ccagatcggc ttggatgacc
     2281 ttggcaatct ccggccgacg caccttcaag gcgacgatcc gcccattcgg caggaccgca
     2341 cggtggacct gagaaatcga ggccgacgcc accggaattt cctcgaccga agcaaacaac
     2401 tcgtcaagcg gccggccgta ctcctctttc aggatcgttg caacctgagc aaaggaaaat
     2461 ggcctcacgt catcgcggag cgcggccagt tcgttggtgt aagtctccgg caaaagatcc
     2521 gggcgcgtac tgagcaactg cccgagcttg acgaaggtcg ggcccaggtc ttcgcacacc
     2581 aggcgaagcc tgcggggccg gtcctgcggc gggacatcgt tgcccagagc aacccgctcg
     2641 atgggtcgga caataccctc gagatggagc gcagcaacca cgtcctgata gccatagcgc
     2701 actaggctag tcaggatttg gcggtagcgg ggaaggtctc ggatatggcc aacggtttcg
     2761 cgaatcagag acataacccc gccaaggcct cgatcggccg tccgactccc gcccaactgc
     2821 aggcgttccc cccgcggtcg atcgaacgga gcggagggcg gcgcgcctgg cttccgggca
     2881 acttgtaacg gtgttcatct tgcacgtctg ccggcttttt ggttaaatta tctcttatga
     2941 ttcaaagata gaaagtggat ggattcggga aacatttcat gccgatccaa caacagtctg
     3001 cgccggttcg ccaactggaa tctgacgagg aattctttga gacacgcttg gaagccatag
     3061 gatttatttt ccacggaacg attgcggatg ccaacggcaa ggctcatacc agccgttcgt
     3121 cttccaatct gttgcacttc gctcggtgcg ccaagctcga gaaagtcggc gacaccgaga
     3181 cgaagatttg gtttcgttcc gtgcgtgtgg ctatcaagca tctcgacgag ttgatcgggc
     3241 acccacgctg gaagtggtgt aaagtttgcg agcgagagat cacgcagcga attcttgacg
     3301 aaacctgaga cgtggctggc tggtgcagcg ctgcaaaaat gatggcttga gcgttgggcg
     3361 tgccgcttta aaactctggt cgaagccagg ccttgaatcc gattttgcgc cccggtcgcc
     3421 gtgatttgtg atgggattgg ttctcgtgct caagccgacg gcgcatgtcc cattaagcgc
     3481 tcaaactccg ggttgccgcg gagatgattg aagtccgcct catcagcaat catggtgcgc
     3541 agttcggcat cgagtgctag cgcacttgtg agtgcatcaa gcgccttgga gctattgcga
     3601 gccaagctcc agtaacaggc caggttgtag tgtaggagcg cctcgtccgg atgctcacgg
     3661 agggcccgct cgagggagtc gatcgcctgg gccaatctgt tagtgcgttt atagcaccag
     3721 cccagcgcca gggcgacccg agtatcggac ggccgcaacc ccgccgcgac ttcgagcggt
     3781 ctaagcgcct cgcggtaatg tttcaagttg cgcagggctt cgcccttcaa gaagctcgct
     3841 tcaaactgca tgctcagcca gtctggccgg ctctcgagga tctggagcga tcgctcggga
     3901 aggtcgagca ttagataacc ttcagcctcg tcgagttgtt tgcggatctg gtcttcgcta
     3961 ttcggagtca tctccgcgcg ctctcccaga aaggccggcg ccgaatcgca cgagcaggga
     4021 cgacgttccg catcatgtcg tggaccaccg cgcaacacgt caccagacgt gccgggcgtt
     4081 cagtcgtttt ggtgcggagc cgtgctttcc gggtcaccca gtcgaagttt acctcaagat
     4141 cacggttgcg gaaagagtgc atgcgacgag tcgtgacgga agcgccttgt tcaggaggtg
     4201 acacccgaag tcgcggcgag agtgcccgcg tgcggggtcg gtttctgagc ctccgtatca
     4261 ccatagtaga ttcgctggat gcgctctcgc gttaagccga gcacccgatc aaatcggctg
     4321 agtttcattc cgtcgagatg tgtcgagtca tccgccagat tttccagtat gagcgcatcc
     4381 tcgtcgacgg ttgttcgaat cttgtgacga acgaaccagc caacgtgctg accgatgtgg
     4441 gcaaaaagcg gccatctcga ttgaaaggag ccgaaggtca cgatcagcgt attcttcgca
     4501 tcttccggca cgaagtaatg aaacaaatg
//
LOCUS       DATAQL010000845         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4666880, whole genome shotgun sequence.
ACCESSION   DATAQL010000845 DATAQL010000000
VERSION     DATAQL010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4666880"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VHS97_14715"
     CDS             complement(<1..285)
                     /locus_tag="VHS97_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449504.1"
                     /translation="MMSVIWAFLIYWLVTFVACFVVAEVGQDQLYDEVTPRVGLKVAS
                     GSFLIAALLTALRYFGVPASFESMFTTNIAWTLLQGIVWFGVFTLILQFHP"
     gene            537..1361
                     /locus_tag="VHS97_14720"
     CDS             537..1361
                     /locus_tag="VHS97_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3449505.1"
                     /translation="MIRLSAFADEISQNPVEQIDVLAAHGIKHIEFRAIHGTNVLDLA
                     DDQHTSFRDLLRSRGVGLSAIGSPIGKIRINEPFDEHLERFGRAMDLADFYQAPRIRV
                     FSFYMPPGDDPSTHRAEVVKRMAELSRRAAARGIRLFLENEKGIYGDTAARVADVLET
                     VNSPSLSHAFDPANYVEVGQPVEEAWSLLRSHVKHFHVKDYDAKIHRNVPAGAGDGQI
                     PTLIADAVARGYDGYCVLEPHLVVAELSFGFTGPERFGDAANALKQILQDRSIAFE"
     gene            1528..1854
                     /locus_tag="VHS97_14725"
     CDS             1528..1854
                     /locus_tag="VHS97_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449506.1"
                     /translation="MADKDEVAKKLAHKHFDIEPGITRIFKLRDRPELEVLSNTPIKL
                     LEVNVDTAPSGIMPLYFGPVPNSGIPYPSVIVEVTPDEFERIKVHELKLPDGWTIDEE
                     YPKGSG"
BASE COUNT          433 a          547 c          538 g          345 t
ORIGIN      
        1 gggatgaaac tggagaatca acgtgaaaac gccgaaccaa acaatccctt gcagcaatgt
       61 ccaggcgatg ttggtcgtaa acatcgattc aaaagacgcg gggacgccga aataccgcag
      121 cgcggtcaag agcgcggcaa ttaagaacga gccggacgcg accttcaagc ccacacgagg
      181 ggtaacctca tcgtaaagct ggtcctgccc cacctcggcc acaacgaagc aggcgacaaa
      241 agtaaccaac cagtaaatca agaacgccca gatgactgac atcataaacg tgcaaccgaa
      301 gtgacccaaa acgcctcagc gaggttttcc acaactctat atcgaatcat accgcacatc
      361 cacggcccag gccactcgcg cctgcacttt tggccgcaat gacctttccc gacagggcgg
      421 gacttccgag aatcgcggca cgaactgacc ccaccctggc ggatgtgcct ccgtctgctt
      481 attatcgatc tcgaccaagt tgttcgcatc gaggtacaaa atccgggaag tgatcgatga
      541 ttcgcttgag cgccttcgcc gacgagatct ctcaaaaccc ggtcgaacag atcgacgtcc
      601 tggcggcgca cgggatcaag catatcgagt tccgcgcgat ccacgggacc aacgtcctgg
      661 atctggcgga cgaccagcat acgtcgtttc gcgacttgct gcggtcgcgc ggggttgggt
      721 tgagcgccat cggatccccg attggaaaga tccgtatcaa cgagccgttt gacgaacacc
      781 tcgagcggtt tggtcgtgcg atggatctgg cggattttta ccaggctcca cgcatcaggg
      841 tgttcagctt ctacatgccc ccgggcgacg atccttcgac tcatcgcgcc gaggtcgtga
      901 agcggatggc cgagctttcg cgccgcgctg ccgcgcgcgg cattcgcttg ttcctggaaa
      961 atgagaaggg aatttacggc gacacggcag cgcgggtagc cgatgtgctc gagacggtaa
     1021 attcgccctc cctctcgcac gcgtttgacc ctgccaacta cgtcgaggtc gggcagccgg
     1081 tcgaggaagc ctggtcgcta ttgcgctcgc atgtaaaaca tttccacgtc aaggattacg
     1141 atgcgaaaat ccaccgcaac gtcccagccg gagctgggga cggtcaaatc cccaccttga
     1201 tcgctgacgc cgtcgccaga ggatacgacg gctattgcgt tctggagccg catctggttg
     1261 tcgccgagct gtcgttcggc ttcacgggac ccgagcggtt cggcgacgcc gcgaacgcgc
     1321 tcaagcaaat cctccaagat cgctcgatcg catttgaata agaatgcggt tctgcagaag
     1381 gcccaggtca agctctccgg cgccagccac ggcacggtcg acgcgaggaa caagctgatt
     1441 ctatgagatc cggtctgtct cggcgttaaa gacctgggca atcccgcgat tctgagacaa
     1501 ggttccggaa ggctggagga tagacttgtg gcggacaagg acgaagtggc gaagaagctg
     1561 gctcacaagc atttcgatat cgagccggga atcacccgaa tcttcaagct ccgggacagg
     1621 cccgagctcg aggtattgtc gaacactccg atcaagctgc ttgaagttaa cgtcgatacc
     1681 gcgccgtcgg gaatcatgcc actctatttc gggcccgtgc caaatagcgg catcccctac
     1741 ccgtcggtga tcgtggaggt cacgcccgac gaatttgaga ggatcaaagt gcatgagctg
     1801 aagctgccgg acggctggac gatcgacgag gagtatccca aggggagcgg atgatggcgg
     1861 atc
//
LOCUS       DATAQL010000846         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_391701, whole genome shotgun sequence.
ACCESSION   DATAQL010000846 DATAQL010000000
VERSION     DATAQL010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391701"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..580
                     /locus_tag="VHS97_14730"
     CDS             191..580
                     /locus_tag="VHS97_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEX3449507.1"
                     /translation="MKTQGEIEAAVCEGMSRFEQEYMGRGPKDVRAHLFDEMLLVRLQ
                     GVLTAAEQHLVKSQPAEKGRDLLKQVRTHLLETARPLMEAMIREITGMKVISMHHDIS
                     TVTGEEVVLFTLAEAPQVRETKKKSTG"
     gene            complement(737..>1882)
                     /locus_tag="VHS97_14735"
     CDS             complement(737..>1882)
                     /locus_tag="VHS97_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3449508.1"
                     /translation="ATLGMGVVACLAYMFIDLEGNEVERGHKAVHNPLKGQATTDGND
                     RVRAEAVQALGKIGETAAVVAVPGLVRALSDRDNWVSALAAEALGEMGDLADGAISAL
                     VRSLSHPNAEVRRNSAEALSKMNGAAAAARPSLEKAARDEDGGVRSEAIRALGAIGAP
                     TPTSTQAVLAGLQDLDPQVRAAAIESVGRWGDTSTAVVGTLLILLDDANDQVKVQATK
                     VLPALAGATPEVIDGLCRRLLEDDSDWVQLYAALAPGKLGPATAASGASLLRAAQTAD
                     VSVREQAMRAIATIQPPETTEALIGGLKDACGDIRRVASAGWMNATAISEEAIPALIE
                     GLRDPEVQVRANCAHALARLDVIPAPAIPHLKQTNRQNQEVEFAVIS"
BASE COUNT          374 a          596 c          556 g          356 t
ORIGIN      
        1 cctgccagaa tgcacagttc cccagttcgc gtcattgtcc cgcgcactgt ttaagtggca
       61 aggaaattgt ttctcacctg ttataatagt tgccagttaa gaggagagcc gacgatccag
      121 gccgcgacgc tctgttcgga aaccatgtcc aagactcgcc tgccgataat ggaacggcgc
      181 cgaggtgacc atgaaaacgc aaggcgaaat cgaagccgcc gtatgtgaag ggatgagtcg
      241 ctttgaacag gaatacatgg gacgggggcc caaggacgtc cgcgcgcatt tgttcgacga
      301 aatgctcttg gtccgcctcc agggcgtatt gactgccgcc gaacagcatc tggtcaagtc
      361 acaaccggcc gagaagggaa gggatttgct caagcaagta cgtacacatc ttctcgaaac
      421 cgcacgaccg ctgatggagg caatgatccg tgaaatcacg ggcatgaagg tcatcagcat
      481 gcaccacgac atcagcaccg tcaccggcga ggaggtcgtg ctctttacct tggccgaagc
      541 gccacaagtc cgcgaaacga aaaagaagtc gaccggctga gcctgaacga aaacggcctg
      601 atctgcgacg acgggcgggc cggcgagatg atcgtggaac acttcgtagt atcttacaca
      661 ccataatata ctgttgttat tcagagtgag ctcaacctac tgtgttccaa atggtcgcga
      721 tctgtcccga tatgcatcaa gagattaccg cgaattcaac ctcttgattc tgccggttcg
      781 tttgcttcaa atgcgggatg gccggggccg ggataacatc gagccgggcg agcgcgtgcg
      841 cgcagttcgc ccggacctga acctccgggt cgcgcagacc ttcgatcaga gcggggatgg
      901 cttcttccga tatggccgtc gcattcatcc agcccgccga ggccaccctg cgaatatcac
      961 cgcaggcatc cttgagaccg ccgataagag cctcggtcgt ctcaggtggt tggatcgtgg
     1021 cgatcgcgcg catcgcttgc tctcgtacgc tcacatccgc ggtctgcgcg gcacggagca
     1081 gcgatgcacc ggatgccgcc gtggcgggac ctagcttgcc cggggccaat gcggcataga
     1141 gctgaaccca gtcgctgtca tcctcgagca agcgacggca caagccgtcg atcacttccg
     1201 gcgtggcgcc ggccagcgcg ggcagaacct tggtcgcttg aacctttacc tggtcattcg
     1261 cgtcatcgag gagaattaac agggtgccca cgaccgccgt gctcgtgtcg ccccaccggc
     1321 ccacggactc gatcgcggca gcccgtacct gcggatcgag gtcttgtaac cccgccagga
     1381 ccgcctgcgt cgaagtcggt gtcggagcgc cgatcgcgcc tagggcacgg atcgcctcgc
     1441 tgcgcacgcc tccatcctcg tcccgcgccg ccttttccag cgacggccgc gcggcggcgg
     1501 ccgccccatt cattttgctg agcgcctcgg ctgagttacg gcgaacttcc gcattagggt
     1561 gactcaggga acggaccagg gccgagatcg cgccgtcggc cagatccccc atctcgccca
     1621 aggcttccgc ggccaaggcg ctgacccagt tgtctcgatc cgagagcgcc cgcaccaggc
     1681 cgggaacggc gacgaccgcg gccgtctctc cgatcttgcc cagagcttgc acggcttcgg
     1741 cgcggactcg gtcattgccg tcggtcgtcg cctggccttt taacggatta tggacggcct
     1801 tgtggccccg ctcgacttca tttccttcca ggtcgatgaa catgtaggcg aggcacgcaa
     1861 caactcccat tcccagtgtc gc
//
LOCUS       DATAQL010000847         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2379454, whole genome shotgun sequence.
ACCESSION   DATAQL010000847 DATAQL010000000
VERSION     DATAQL010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379454"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            724..2046
                     /locus_tag="VHS97_14740"
     CDS             724..2046
                     /locus_tag="VHS97_14740"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX3449509.1"
                     /translation="MPALQSQPNSAARELFTEISRWPIYDPHTHIDARRPAARHLDEI
                     MGYHYYTELAHSAGMPTEEVSPDLDPLVRVRNLVARLDSIDSTVQYSWLLEIARTFYQ
                     FPHDRITPGNVEELVDHADHERDGTEWDKEVWKTTRLEAVFLTNEFDDTLDGWDTAAY
                     VPCLRTDDLVLKLHDQKTVGRLRAASDVDVRDCASLREAIGRLFDRFVKRGARACAIS
                     LPPDFSPRRASPRRSVTPIRKAIHGMDLRPDELEEVRSSVFWMLAEFCAEFRLPFDLM
                     IGPLRNVYPLGVAGGRDLFDRRVSLYDYRELFNHFGNVTFPVSTLAPDAGGELVAYSW
                     IFPNVLPMGHWWYSNVPTYIAADLRARVQALPKIKLLGYYSDAYKLEFILPKFNMYRR
                     ILAEVLSGEVIGGRGWSVDEALQLAKLILIDNPKRIFGRTSPSSKDVI"
     gene            2183..3751
                     /locus_tag="VHS97_14745"
     CDS             2183..3751
                     /locus_tag="VHS97_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449510.1"
                     /translation="MFSTKDGITKRAGLVFILSLLLEGPAFAQMTSGPGLSSGSGAGT
                     GAGGSSGVLRNRSGSLSGVGPGPAGAGMRGAPLPPLTGSRCQPPTSFGPGVDVTFPND
                     PFLIPFMTMETSGIAVDSEAPVRITADLLKDARMITDPEERSLALQRIANGAIASNPP
                     QWSLAHKTLEEAITATSEVKTPLVRDQRLIAIVTSLTFLSDGLLRVGREPQEKLAQME
                     PDVSGPEALPKKFDANVLIRLARLEWRRAVYLASIIDNPTYRNEMLYKVSESQASGSA
                     SIANDYSVAVELRSLADRVPADDKTKGEKQPGLDDQAREAQAANAAFKKLADAILVDS
                     FEDAKKIDRLIWKYRAMVRIALSAADSQQYERGIELSRGINNGESRAEAMLLLAESMC
                     RKNQKELNERATVPYEEAARAVASVQQEGLRGVLVGFLVDSLIATGRFSDARACAELH
                     PDPAITLVALGAVAESQGRRGSAEAARRWIATEIPAQHRSVLYRRVTIGALSAIEQNR
                     SKDFNLQDGAMPRR"
     gene            complement(3832..>4383)
                     /locus_tag="VHS97_14750"
     CDS             complement(3832..>4383)
                     /locus_tag="VHS97_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX3449511.1"
                     /translation="YATVMRPLEAGAGGVMISMVQSPADVEKAVRWAKFWPRGERGLN
                     GGNRDGRFGLTPIAEYTARANAETFFGVQIETAGALASLNEIAAVPDVDLLFVGPSDL
                     SQVLGVTGDFDHPKCMNAIQAIARACADAKKPWGIFSRGPDYVAKMQSWGCQLFVLAA
                     DLHVVHAGIRSIKERYATFFPRG"
BASE COUNT          868 a         1315 c         1320 g          880 t
ORIGIN      
        1 ctttctggtc gaccggctca agctcgacgg ctttgcgcag acgaactcag gcccctggca
       61 caatgcggtc gtctaccggc agccgattca gtgacctcgg ccgagcagga tgcgagatta
      121 cggccggcga tcgaagcaag cgctagccgt caagcgaatc ttcccgggat gacatgatat
      181 cgatcacaaa aacacgaaag agcgaaaaca cgaaagaaga tgtcagactc gggtcccaat
      241 agggccgaag ttgcgaaatt gcggatgctc tcctagtcgg gctcgtttgg tgcctttgat
      301 cggttcgtga ctttttcgtg ctttcgtcgt ttcgtgctct tgtgatctgc aggtatgcgt
      361 tactgaatga agccagtttc gcatggtcgg agatgcggcg ccatcgtcgg cgtatccttc
      421 agaggttctc ctacggctgt ttccgagtcg cacaattcat gcaaccgcgc agccccctct
      481 aaccgacatc cgcacagagg gcacctcccc acccgcacct gttcggtaat attcaatttt
      541 aagtcggcgt cccggacctg gtttgccccc acgcgggcta ttgcgaggga cggcggtgac
      601 ggctagcctg tgctggtcgc gttcctctat ctattttatc gcgcttgcgc gacgtaggtc
      661 gggctttttc agcccgacgt cagactggaa tggctgacct actcttaagt tgcaggacgg
      721 aatatgccgg ctcttcaatc gcagccaaat tcagcggccc gggaactttt cactgagatc
      781 tcgcggtggc cgatttacga cccgcatacc cacatcgatg cgcgccggcc cgccgcgcgt
      841 catctcgatg agatcatggg ttatcattac tacaccgagc tcgcccattc cgccggaatg
      901 cccaccgaag aagtctcacc ggacttggac cccctggtgc gcgtccgcaa tctcgtcgcg
      961 cgcctcgata gtatcgactc gacggtccag tattcgtggc tgctggagat cgcgcgaacc
     1021 ttttatcaat ttccgcacga tcggatcacg cccgggaacg tggaggagct tgtggatcac
     1081 gccgaccacg agcgcgacgg cacggagtgg gacaaagagg tctggaagac cactcggctg
     1141 gaagctgtgt ttctcaccaa cgagtttgac gacaccttgg acggttggga caccgcggcg
     1201 tatgtgccgt gcctgcggac cgacgacctg gtcttaaaat tgcatgacca gaaaacggtg
     1261 ggccgcttgc gggctgcgtc ggacgtcgat gtgcgcgatt gcgcgagcct gcgtgaggcc
     1321 atcggccgtt tgttcgaccg cttcgtcaag cgcggcgcgc gggcctgcgc cattagcctg
     1381 ccgccggact tttctccgcg tcgggcgtca ccccgacgct cagtgacgcc cattcgcaag
     1441 gcaattcacg gcatggacct acgtcccgac gaactcgaag aggtgcgatc gagtgttttc
     1501 tggatgctgg ccgagttttg cgccgagttt cggctccctt ttgacctcat gatcggaccc
     1561 ttgcgcaatg tctatcctct gggcgtcgcc ggtgggcggg acctgttcga tcgccgggtg
     1621 agcctctatg attacagaga gctgttcaat cacttcggca atgtcacgtt tcctgtttcg
     1681 accctggcac ccgacgccgg gggagagctc gtggcttatt cgtggatctt tcccaacgtg
     1741 ttgccgatgg ggcactggtg gtactcaaat gtgccaacat atatcgcagc cgaccttcga
     1801 gcgcgagtac aagctttacc taagatcaag ctgctgggat actattccga tgcgtacaag
     1861 ctcgaattca ttctgcccaa attcaacatg taccgtcgaa ttctggcaga ggtcttgagt
     1921 ggagaagtga tcggcgggcg tggctggtca gtcgatgagg cattgcaact ggcgaaactg
     1981 atcctgattg acaaccccaa gcggattttc ggcaggacct ctccgagttc caaagatgtt
     2041 atttgaccgg aagccgatct caaggcatgc ttcgatcacg cgatgcgatc ggtttccggt
     2101 tcgcggtccg gggcgtacct gccgctccgg tcctcggttc gcgattgaag gccggacctg
     2161 aagaggtcgg gagtacagcc gcatgtttag cacaaaagat ggcatcacaa agagggcggg
     2221 tctcgtcttc atcctgtcac tgttattgga ggggcccgcg ttcgcccaga tgacaagtgg
     2281 gcccggactc tcgtcaggca gcggagcggg aacaggggca gggggatctt cgggcgtact
     2341 gcgcaaccgg tccggttcgc tttcgggtgt gggaccaggt ccggccggtg cgggtatgcg
     2401 cggcgctcct cttccgcccc taacgggatc gcgatgccag ccgccgacgt cattcggacc
     2461 gggagtcgac gtgaccttcc ccaacgaccc tttcttgatc ccgttcatga cgatggaaac
     2521 gtcagggatt gcggtcgata gcgaagcacc cgttcgcatc acggccgatt tgctcaaaga
     2581 cgctcgtatg atcaccgacc cggaagagcg gagcctggct ctgcagcgca tcgccaacgg
     2641 cgcgatcgcg agcaaccctc cccaatggtc gctggcgcac aagacccttg aggaagccat
     2701 cacggccacg tccgaggtca agaccccgct cgtgcgcgac cagcggctga tcgcaatcgt
     2761 gacgtcactg acctttttga gtgatgggct gttgcgcgtc ggacgtgaac cacaggaaaa
     2821 actcgcacag atggagccgg atgtatcggg ccccgaagcg ctgccgaaaa agttcgatgc
     2881 caacgtgttg atccgtttgg cccgcctcga atggcggcgt gcggtctatc tcgcttcgat
     2941 catcgacaat ccgacgtacc gcaacgaaat gctctacaag gtgtccgaga gccaggcgtc
     3001 tggcagcgct agcatcgcca atgactactc tgtcgcggtt gagctgagat cgctcgccga
     3061 ccgagtgccc gccgacgaca agaccaaggg tgaaaagcaa ccgggtctcg acgaccaggc
     3121 ccgcgaggcg caggcggcga atgcagcttt caagaaactg gctgacgcca tcctggttga
     3181 ctcgttcgag gacgccaaga agattgaccg cttgatatgg aagtatcgcg ccatggtgcg
     3241 gatcgcactg tcggccgccg attcccagca gtatgagcga ggcatcgaac tttcgcgcgg
     3301 gatcaacaac ggtgagtcgc gggccgaggc catgctgctt ttggccgaat cgatgtgccg
     3361 gaaaaatcaa aaggaactca acgaacgtgc cacggtccct tacgaagaag ccgctcgggc
     3421 cgttgcatcg gtccagcaag agggcttgcg gggcgtgctg gtcggcttcc tggtcgacag
     3481 cttgatcgcg accggccgat tctcagacgc gcgggcgtgc gccgagcttc acccggaccc
     3541 agccataact ttggttgcac tcggggccgt tgcagaatcg caaggccgcc gcggatcggc
     3601 cgaggcagcc cggcgctgga tcgccacgga aatacccgca caacataggt ccgtactcta
     3661 ccgccgcgtc acgatcgggg cgctctccgc gatcgagcag aatcgaagca aggattttaa
     3721 cctgcaagac ggggctatgc ctcgcagatg atgccggccg accggattgc cgcgtcgcga
     3781 tcggcccttg aaaagcggga aagcctgaga gagagccgga ccgatcgatc atcaaccacg
     3841 cgggaagaac gtggcgtagc gttccttgat cgaccgtatc cccgcgtgca cgacatgaag
     3901 atcggcggcg agaacgaata actggcaacc ccagctttgc attttggcca catagtcggg
     3961 tccgcgactg aagatgcccc agggcttctt ggcgtccgcg caggcgcgcg ctatggcttg
     4021 gatggcattc atgcacttgg gatggtcgaa atcgccagta acgccgagta cttggctcag
     4081 gtcggagggt ccgacgaaca gcagatcgac gtcgggcacc gccgcaatct cgttcaaaga
     4141 cgcaagggcc cccgccgtct cgatctggac gccgaaaaag gtctcggcgt tggcccgggc
     4201 ggtgtattca gcgataggag tcaagccgaa ccggccatcg cgattgcctc cgttcaaacc
     4261 acgctcgcct ctgggccaga atttggccca ccgcacggct ttttcgacat ccgcgggcga
     4321 ctggaccata ctgatcatga ctcccccggc ccccgcctcg aggggccgca tcaccgtcgc
     4381 gta
//
LOCUS       DATAQL010000848         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4057022, whole genome shotgun sequence.
ACCESSION   DATAQL010000848 DATAQL010000000
VERSION     DATAQL010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4057022"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VHS97_14755"
     CDS             complement(<1..1784)
                     /locus_tag="VHS97_14755"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449512.1"
                     /translation="MAARSTASRNPSKASARTTIAVQGARVHNLKNISVEIPRDKLIV
                     ITGLSGPGKSSLAFDTIYAEGQRRYMESLSSFAKRFVPQVAKPDVDFVFGLSPVISIE
                     QKTIASNPRSTVGTMTDIASYLNLLVATIGEPHCPRTGEPTPSRTASQILEAILSLPE
                     GAEIELGAPVFKVYGEELDFVLTEVRKKGCRRLIIDGQPVDISEEVELDESKVRHMDA
                     VVDRLAVSRKHEKAIKAGIAATLLVGDGLLQARVVKGANKIQCERFYQGLCSATHHFV
                     YGDIQPEYFVFNNPESACRTCGGLGVYKLTHPELLVPDPKRSIRGGCFVREAFKYNPD
                     TWDGRVMYSLAKGLRFSLDTPWEKLPESVRQAILNGIDPKKVILSPPPEAKGSHDDWE
                     GKEVGFGGIARRIERRYRRYRQRGEANSGMEAWLDNVMVEHTCPDCNGARVRATRLLF
                     TVAGKTIYELGQLNFDELHAFLATVEPTGRGADAGRQVIKEIRGRLKLLLGIGLDYLN
                     LNRRSGTLSGGESQRIRLSTQIGSGLMGMLYVLDEPSIGLHPKDNVKMIATLESLRDI
                     GNTVIVVEHDEDTIRAADHLVEMGPGPGV"
     gene            complement(1774..>2585)
                     /locus_tag="VHS97_14760"
     CDS             complement(1774..>2585)
                     /locus_tag="VHS97_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449513.1"
                     /translation="AKSDIDLLLVTIDDKKVAEGYLALYADGLNVHAVLVPRAQFRKT
                     MEGAIRNSFMHSLVAKGRLLYTHDETITDLCARLGGIGERDTQVQLLNAATRALGPIY
                     KAHKFLVTRGDLDYTALWILYAATPLAQIEVIGRRLLADREVIPQAMKLNPAFFKTVF
                     ADLLNGKKTRKNVEAALESVDRYLAERAAKLFAPVINHLREVGEARSCTEIEGHFQRN
                     LNVSGVYAACEYLADQGLIGKASTPVQLTKKSNIEVQELAFFYLEERSDGC"
BASE COUNT          440 a          839 c          788 g          518 t
ORIGIN      
        1 actcccggtc cgggacccat ctccaccagg tggtcggcgg cgcggatggt gtcctcgtcg
       61 tgctccacga cgatgaccgt gttcccaatg tcgcgcaggc tctcgagcgt ggcgatcatc
      121 tttacattgt ctttcgggtg caggccgatg ctcggctcgt cgagcacata gagcatcccc
      181 atcagccccg acccgatctg tgtggacaga cggatccgct gcgactcacc gccggaaagc
      241 gtgcccgacc gacggttcaa gttgaggtaa tccaatccaa tgcccaaaag cagcttcagc
      301 cgaccgcgga tctccttgat cacctggcgg ccagcgtcgg cgccgcggcc ggtcggctcg
      361 accgtggcca ggaacgcgtg gagttcgtcg aaattgagct ggccaagctc gtagatggtc
      421 ttgccggcaa cggtaaagag tagtctggtg gccctgaccc gcgcgccgtt gcaatccggg
      481 caggtgtgct cgaccatcac gttgtcgagc caagcctcca tcccggagtt tgcttcgccc
      541 cgctgccggt atcggcggta gcgccgctcg atccgccggg cgattccgcc gaagccgacc
      601 tccttgccct cccagtcgtc gtggcttccc ttcgcctccg gcggcgggga aaggatgacc
      661 ttcttggggt cgatgccgtt caagatcgcc tggcgcaccg actcggggag cttctcccac
      721 ggtgtatcga gcgagaaacg cagccccttc gccaggctgt acatcactcg tccgtcccag
      781 gtgtcggggt tgtacttgaa cgcctcgcgc acgaagcaac cgccgcggat gctccgcttg
      841 ggatcgggaa ccagcagctc tgggtgtgtg agtttgtaga cccccagacc accgcaggtc
      901 cggcaggcgc tctcagggtt gttgaacacg aagtactcgg gctggatgtc cccgtagacg
      961 aagtggtgcg tagcactgca cagcccctgg tagaagcgct cgcactgtat cttgttcgcg
     1021 cccttgacga cccgggcctg gagcagccca tcaccgacca ggagggtcgc ggcgatcccg
     1081 gccttgatcg ccttctcgtg ctttcggctg accgccaacc ggtccaccac tgcgtccatg
     1141 tgccggacct tcgattcgtc gagttcgacc tcttcggaga tgtccactgg ctggccgtcg
     1201 atgattagtc gccggcaacc cttcttccgc acctcggtca agacgaagtc cagttcctcg
     1261 ccgtacactt tgaacacggg cgcacccagc tcgatctccg ctccctccgg gagcgacagg
     1321 atcgcctcca gaatctggct cgcagttcgg ctgggcgtag gctcgccagt gcgcgggcag
     1381 tgcggctcgc cgatcgttgc caccaggagg ttcagatagc tggcgatatc ggtcatggtg
     1441 ccgacggtcg aacgcgggtt actggcaata gtcttctgct caatggagat caccggcgac
     1501 aggccgaaga caaagtccac gtccggtttg gcgacttggg gcacgaacct cttcgcgaag
     1561 ctggagagcg actccatgta cctccgttgc ccctcggcat agatggtgtc gaacgccaag
     1621 ctcgacttgc ctgggcccga aagcccggta atcacaatga gtttgtcgcg cgggatctcg
     1681 accgagatgt tcttgaggtt gtgcacgcgc gcgccctgca cggcgatggt cgtgcgcgcc
     1741 gaggccttcg acgggttccg gctggcggtc gacctagcag ccatccgatc gttcctcgag
     1801 gtaaaaaaag gccagctctt gcacttcgat gttgctcttt ttggtgagct ggaccggcgt
     1861 ggaggccttg ccgatcaacc cctggtcggc caaatactcg cacgcggcat agacgccgct
     1921 cacattcagg ttccgctgga aatggccctc gatctcggtg catgacctcg cttcgccgac
     1981 ctcgcggagg tggttgatca caggtgcgaa cagtttcgct gcgcgctcag ccaggtagcg
     2041 gtcgaccgac tcgagcgcgg cctcgacgtt tttccgcgtc tttttgccgt tcaagaggtc
     2101 ggcgaagacc gtcttgaaga acgccgggtt cagcttcatc gcttgaggaa ttacctcccg
     2161 gtcggcaagc aatctccggc cgatgacctc gatttgcgcc agcggcgtcg cggcgtagag
     2221 aatccacagc gccgtgtagt ccagatcgcc gcgcgtgaca agaaacttat gggccttgta
     2281 gatcggcccc agagcgcgcg tcgccgcgtt caagagctgt acctgcgtgt cgcgctcacc
     2341 gatccccccg agccgagcgc agaggtccgt gatcgtctcg tcatgggtgt agagcagccg
     2401 ccccttggcc acgagcgagt gcatgaacga gttgcgaatc gcgccttcca tcgtcttgcg
     2461 gaactgtgcc cgcggcacca ggacggcgtg cacgtttaac ccgtccgcgt agagagccag
     2521 gtaaccctcc gccaccttct tgtcgtcgat ggtcacaagc agcagatcga tgtctgactt
     2581 ggccc
//
LOCUS       DATAQL010000849         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5431371, whole genome shotgun sequence.
ACCESSION   DATAQL010000849 DATAQL010000000
VERSION     DATAQL010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5431371"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..565
                     /locus_tag="VHS97_14765"
     CDS             134..565
                     /locus_tag="VHS97_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449514.1"
                     /translation="MIEASVTIEVAAHPGKALTRKAASESPTRAGKLAAAKPPAHTSA
                     TGSTAHTSATESTAHTSAAHVATTEPAAHVSATAKPPAVSTPTTVSTPGSPAARERVS
                     GQSLGESGSRRQNDHGLASHWIYSFGRDVVHSTENPEWAFH"
     gene            complement(859..>1812)
                     /locus_tag="VHS97_14770"
     CDS             complement(859..>1812)
                     /locus_tag="VHS97_14770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449515.1"
                     /translation="NAGAKLDWFGFGTLSLAIGALQVLLDRGEQLDWFGSGEIWIEAI
                     VAASALYLFLVHIFTSDEPFVRPALFRDHNFAAGTLFIFIVGVTYLASLALLTPYLQS
                     LMNYPIVTAGLVMGPRGIGTMGAMLIVGRLVGRVDTRFLLAVGLGLTAWSFYQMTGWT
                     PDVSQTTIIVNGVVQGAGLGFLFVPLSTVTLGTLPPEQRTEGAGLYNLSRNIGSSVGI
                     SLVTSLLTQNTQANHADIVAHVTAVNRMFESPTIAQFWNPFTDAGRAALDAMITRQAQ
                     IIAYIDDFKLLMIATLIVIPLLIVFKKASDGVGEDHTLVVE"
BASE COUNT          400 a          558 c          544 g          310 t
ORIGIN      
        1 atggctagta gtacgagggc ggatagcagt tgtagttaga cgtgtaatag ggatagccgt
       61 aactgcacgc gtaatcgtag acgccgtaat cccagtccgg ctcgaaaaag aaaccgcgac
      121 ggaagcgccg gtcatgatcg aagcatctgt cacgatcgaa gtggcggcgc atcccggcaa
      181 agcgctgacc cgcaaagccg cgagcgaaag cccgaccagg gccggcaaac tggccgcggc
      241 taaacccccg gcccatacga gcgccaccgg ctccaccgcc cacacgagcg ccaccgaatc
      301 caccgcccat acgagcgccg cccatgtggc caccaccgaa cccgccgccc atgtgagcgc
      361 caccgccaaa ccccccgccg tgtccacccc caccaccgtg tccacccccg gctcccccgc
      421 cgcgcgcgaa cgcgtcagcg gtcagtccct cggtgagagc ggcagccgtc gccaaaacga
      481 tcatggtctt gcgtcacatt ggatctactc cttcggacgc gacgtcgtcc attcgactga
      541 aaacccagag tgggcctttc actaaggtgt ggacgaacgg ctcaatgcaa atcgctatcc
      601 gttgcatgca gtattgagag aaattcagca aactgattgc ggtgacttgg ccgatctaac
      661 gccgctcgtc gttgtcgctg gtcaacttaa gtttcagggt cgggcgcaac gacaaggtcc
      721 gctaagggtc ttgattgtgt gaaactccgg tactcgccgc gctaacctaa ttttttgatc
      781 gaggcggctg gcaaccccgc gcgcgcttgc cccccgcgtt acctttttct tagaggaagg
      841 acctatagag gctagatgtc attccacgac gagcgtgtgg tcctcaccga caccgtcgga
      901 cgccttcttg aagacgatga gcagcgggat cacgataagc gtcgcgatca tcaacagctt
      961 gaaatcgtcg atataggcaa tgatctgggc ctggcgcgtg atcatggcat cgagtgcggc
     1021 gcgaccggcg tcggtaaatg gattccagaa ctgtgcgatg gtcgggctct cgaacatccg
     1081 gttcacagcc gtgacgtgcg caacgatgtc ggcgtggttg gcctgcgtgt tctgggtcaa
     1141 caagctggtc accagcgaga tgccgacgct ggagccgatg ttgcgcgaca gattgtagag
     1201 accggcacct tcggtccgtt gctctggcgg cagtgttccc aaggtgaccg tgctgagcgg
     1261 cacgaacaga aagccaaggc cggcgccctg gaccaccccg ttgacgatga ttgtcgtctg
     1321 cgaaacatcc ggggtccaac cggtcatttg gtagaacgac caggccgtaa gcccgagacc
     1381 aacggccagc aggaagcgcg tatcgacccg tccgaccagc cgcccgacga tcaacatggc
     1441 gcccatcgtg ccaattcccc gaggccccat gaccagtccg gcggtgacga tcggatagtt
     1501 catgagactc tgaagatacg gggtgagtag cgccagcgag gcgagatacg tgacgccaac
     1561 gatgaagatg aagagcgtac cggccgcgaa gttgtggtcg cggaaaagtg ctggccgcac
     1621 aaaaggctca tccgaggtga agatgtgcac gagaaacaga tagagtgccg aggcggcgac
     1681 gatggcctcg atccagatct cgccggagcc gaaccagtcg agctgctcgc cgcggtcgag
     1741 caggacttgg agcgcgccaa tggcgaggct tagggtaccg aagccgaacc agtccagctt
     1801 cgcaccggca tt
//
LOCUS       DATAQL010000850         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_87173, whole genome shotgun sequence.
ACCESSION   DATAQL010000850 DATAQL010000000
VERSION     DATAQL010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87173"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VHS97_14775"
     CDS             complement(<1..223)
                     /locus_tag="VHS97_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449516.1"
                     /translation="MTRITTGLLIALLAITDPGEAYALITGGEGNKPISDPGWHAGAA
                     AIFNHTGRVAWWEGPPFGGGQWHAECRGDA"
     gene            complement(319..954)
                     /locus_tag="VHS97_14780"
     CDS             complement(319..954)
                     /locus_tag="VHS97_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3449517.1"
                     /translation="MCESGGDDEGWSSASGEVLMAITYEDAVAKVHEWTDNPALRNHA
                     RGVEAAMRRAAHRHGAGAADEPRWAIAGLLHDADYDRWPEEHPRRIVAWLREQGEPEV
                     AQAVVAHYNPADVPELTPLDKALLACDELTGFVMACCLVRPDGIHSLTPSSVKKKLKD
                     RSFAAKVKREDVYGGVERLGVNLDEHIQLVIDALAPHAEALGIGGAERSSS"
     gene            complement(1034..2335)
                     /locus_tag="VHS97_14785"
     CDS             complement(1034..2335)
                     /locus_tag="VHS97_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449518.1"
                     /translation="MADCRALDLASGRRLRTRVAGIFLFLPLLARLHFDQIVHRAGYP
                     GSQMVPATGALLSLLTLKLLDKERRSHISDFNFDEALGLFAGLNVLPKATFATDYSYR
                     TQRRHQQQLLSGWISALAPLLFPQGKIFSLDFHAIPFRGDPAALDNHFLPRRGKAGPS
                     VLSFFAQESESRVLCYANANLTRADQSGELMRFVEFWRGLTGHDPEWLYFDSKVVPYP
                     ELSRVNQRGIHFVTIRRRGAAVIRRLRRLPPHTWKRAVIDTPKRCHQLVRFVDEMVRL
                     PGYDGAIRQLAVDGLGREQPTLFVSNNLAETARSLILRYAGRNAVEDGLGTSVNFFHL
                     DCLSSEVRLNVDLDAVLAVLANGCYRWLAHHLHGFDKTAPKKVYRKFVETGGLVEVRS
                     DRILVRFDKRSHNPILREAELDKGCPPIPWLGGRPVAFAYP"
     gene            complement(2290..2607)
                     /locus_tag="VHS97_14790"
     CDS             complement(2290..2607)
                     /locus_tag="VHS97_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449519.1"
                     /translation="MDDSRCLRFFLEPTDTLHRRYEALRAYFVERRPLKEIAQQSGYS
                     YNTLRGVIGAFRTRCREDRVPPFLRHPDRGDRNRTARSRPSPRMPRPWRTVGRWTSPR
                     GAA"
BASE COUNT          489 a          868 c          972 g          472 t
ORIGIN      
        1 tggcgtcgcc gcggcactcg gcatgccact ggccgccgcc gaagggcggg ccttcccacc
       61 aggcgacgcg gcccgtgtgg ttgaagatcg cagccgcccc ggcgtgccag ccgggatcgc
      121 tgatcggttt gttgccttcc ccacccgtga tcaatgcgta cgcctccccg ggatcggtga
      181 tagccaggag ggcgatcagg agccccgtgg tgattctggt catgggatcc cccggtagca
      241 tgcggagagc acacgaggat ctacggacga gagtgagttt tgttagccga gatttcgtga
      301 tcgtagcagg gccggtcgtt atgacgacga ccgctcggca ccgccgatgc ccagggcctc
      361 ggcatgcggc gccagggcgt cgatgacgag ttggatgtgc tcgtccaggt tcacgccgag
      421 ccgctcgact ccgccgtaga cgtcttcccg cttgaccttc gcggcaaagc tcctgtcctt
      481 gagcttcttc ttgacgctcg acggggtgag cgagtggatc ccatccgggc ggacgaggca
      541 gcaggccatg acgaagccgg tcagctcgtc gcacgccagg agtgccttgt cgagcggggt
      601 cagctcgggg acatccgccg ggttgtaatg ggcgacgacg gcctgggcga cctcgggctc
      661 gccctgctcg cggagccagg cgacgatccg ccgcgggtgc tcttcgggcc agcggtcgta
      721 gtcggcgtcg tgcagcaagc cggcgatggc ccaccgcggc tcgtcggccg cgccggcgcc
      781 gtggcgatgc gccgcccgtc gcatcgccgc ctcgaccccc cgggcatgat tccgcagcgc
      841 cgggttgtcc gtccattcgt ggaccttcgc caccgcgtcc tcgtaggtga tcgccatcag
      901 aacctcgccg ctcgccgaag accatccctc atcgtcccct ccgctctcgc acatggcgaa
      961 tccgccccgc gttcatcccc gattatcgcg gcccgaaacc tacctacgat gtgacgtctg
     1021 tccgaggatg acgtcagggg taagcgaagg ccaccggtcg gccacccagc caagggatcg
     1081 gagggcatcc cttgtccaac tcggcctcgc gcaggatcgg gttgtggctc cgcttgtcga
     1141 agcgcaccag gatgcggtcg gaccgcactt ccaccaggcc gccggtctcg acgaacttgc
     1201 ggtacacctt cttcggagcg gtcttgtcga agccgtgaag gtggtgcgcc agccagcgat
     1261 agcaaccgtt ggccagcacg gccagcaccg catccaaatc gacgttcagc cgcacctcgc
     1321 tggacaagca gtccaggtgg aagaagttga cactggtccc caggccatcc tccacggcgt
     1381 tgcgcccggc atagcggagg atcaaggatc gggccgtctc cgcgaggttg ttggacacga
     1441 acaaggtggg ctgctctcgt cccaggccat ccacggccag ctggcggatc gccccgtcgt
     1501 agccagggag ccggaccatc tcgtcgacaa agcgaacgag ttgatgacag cgtttgggag
     1561 tgtcgatcac ggcacgtttc cacgtatgcg gcggcaaccg gcgcaggcga cggatcactg
     1621 cggcgccacg tcggcggatc gtcacgaaat ggatcccacg ctggttgacg cgggagagtt
     1681 ccggataggg gaccaccttg ctgtcgaagt agagccactc cggatcatgt ccggtcagcc
     1741 cccgccagaa ctcgacgaat ctcatcagtt cccccgattg atcggcgcgg gtcagattgg
     1801 cgttggcata gcacaagacc cggctttcgg actcttgcgc gaagaagctg aggacactgg
     1861 gacccgcctt gccgcggcgg ggcaggaagt ggttgtccaa agcggcggga tcgccccgga
     1921 acgggatggc atggaagtcc aacgagaaga tcttcccctg ggggaacagc agtggtgcca
     1981 gcgcggagat ccagccggag aggagttgct gttggtggcg ccgttgggtg cggtaggagt
     2041 agtcggtggc gaacgtggcc ttgggtagga cgttgaggcc cgcgaagaga ccgagggcct
     2101 cgtcgaagtt gaaatcgctg atgtgactgc gccgttcctt gtcgagcaat ttcagggtga
     2161 gcaggctcag cagggcgccg gtggcgggca ccatttgcga gccgggatag cccgcacgat
     2221 ggacgatctg atcgaagtgg aggcgggcca acaagggcag gaacaggaag atcccggcga
     2281 cccgagtcct caggcggcgc cccgaggcga ggtccagcgc ccgacagtcc gccacggccg
     2341 gggcatccgg ggcgacgggc gcgagcgggc ggtcctgttg cggtcgcccc gatcggggtg
     2401 ccgtaaaaag gggggaaccc ggtcttcacg acaccgagtg cgaaaggcgc cgatcacgcc
     2461 ccgcagggtg ttgtagctgt agccggattg ctgggcgatc tccttcaggg gacgtcgctc
     2521 gacgaaatag gcgcggagcg cttcatagcg gcggtggagc gtgtcggtgg gttcgaggaa
     2581 gaagcgcagg cagcgtgagt cgtccacgat ctggccccca cgatgtcggt cgatttccgg
     2641 tagattcccg gcccctgatg atattgattg agaccgatgg gtccaagagg tagcgtcgtg
     2701 ggaaggggcg cggagtcctg gaaacagggc gaaaacgagt atccgagggg accacggcct
     2761 gtcgcatgcc tcttcgattg gagagcgatg aatgccattg g
//
LOCUS       DATAQL010000851         5553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3140449, whole genome shotgun sequence.
ACCESSION   DATAQL010000851 DATAQL010000000
VERSION     DATAQL010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3140449"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VHS97_14795"
     CDS             complement(<1..332)
                     /locus_tag="VHS97_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449520.1"
                     /translation="MQAFGMLLLAAATAGDGFGYNDPGVHLKKWLRPHAVTATDATGA
                     PPYTAASLAGPNGPGAHNGRRFTVTKSQIYFLDPNGMQIGWQTGSGPNGERTYLPAQL
                     TVPARYNF"
     gene            614..1708
                     /locus_tag="VHS97_14800"
     CDS             614..1708
                     /locus_tag="VHS97_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449521.1"
                     /translation="MALVVIVACCLLSQSLAQVPQMQPGAAGAAGATAPDQPVTPGAP
                     PADKPDEPPTKAELLIDDAIIKIAKLESVAADLLQEINMLNQKYTITGRYLKAPNTHV
                     YLKLSVSGLPDTSATSLQVCDGETLWDYQVVLDSQNYRKLSIKPILERLNSPELNERI
                     KDQALTSMGLAGPETLLVGLRKTLKFTQIEEEEVDGKKLWRLRGTWRTRQGLVGPDGR
                     PMGAAGFLPPYIPMDGTLYLGKDDSWPYKLVLVGRKPSALFETRRLGPYGRPIGAKSS
                     IEKINPSEIRLTYSDVKLNAKIRLDEFAFQAPPAAVVDDSTEAIVKGLDQAISMETQR
                     KKSEAAKKEGPVLDRSIDIPSPPDLNAPRP"
     gene            1801..3582
                     /locus_tag="VHS97_14805"
     CDS             1801..3582
                     /locus_tag="VHS97_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEX3449522.1"
                     /translation="MEPTKPGTTPKPSKVEAIKLASEYLKTYVADELNNGSTHFTEDA
                     ATVLKFHGSYQQDDRDLRKQLKREGKDQAHQFMVRVRIVGGKLNALQYLACDHLAQAI
                     GNGSLRITTRQEFQLHGVLKHDLQATMRQINEALLSTLAACGDVERNVLCCPAPLYDS
                     IRDDLNADALAWAAHCAPRSSAYWEIWLDGEKIESLPPDASIEVPTPGDDPVEPIYGK
                     TYLPRKFKTAFALPEDNCTDIHANDLGFLGVVVDGRLAGYNVLAGGGLGTTPSAQKTF
                     PFLAQPLCYVDRGDVKKIGEAVLKVFRDFGNRSDRKRARLKYIIHDWGMPAFRAKVEE
                     YLGHSLADPKPIKVTGVDDHLGWHPQGNGRLFLGIPVENGRIKDDGDFRLASGLRTFF
                     RKYQTSARLTCQQSILLEDIEPSRRGEIECWLEEYGIAAVEQISTVRRWSMACPALPT
                     CGLAVTEAERALPGILDQLEVELARLGLEKERFTVRMTGCPNGCARPYNSDVGLVGRS
                     AHINKDCTPGPGTYTIFLGGRTVGDRLNIEFKDYVPYDQVVAELMPVFSRFKAERNDG
                     ESFGDFCDRIGVLELARSAAPELNPVG"
     gene            3613..4059
                     /gene="cdd"
                     /locus_tag="VHS97_14810"
     CDS             3613..4059
                     /gene="cdd"
                     /locus_tag="VHS97_14810"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX3449523.1"
                     /translation="MEIDEPARADLIAAARQAAGQAYCPYSRFRVGAAVLTDRGEIFS
                     GCNVENASYGLTICAERNAIFQAVARSQAPVVIRAVVVFTPTDLPTAPCGACRQVINE
                     FGPEAFVLSVCDGEEVIEIRLSELLPRAFGPANLEPLRKTENTASR"
     gene            4261..5127
                     /locus_tag="VHS97_14815"
     CDS             4261..5127
                     /locus_tag="VHS97_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX3449524.1"
                     /translation="MRRIAVLNQKGGVGKTTTAVNLAAALAMGGHKTLVLDLDPQAHA
                     TLHLGLLPGRSGPSLYEVLTEGLPLASVRRSVNPNLSICGSHIDLAGAELELLGTVGR
                     EVILRDQLAADTESFDYVLMDCPPSLSVLTLNALCAANEVLIPLQAHFLALHGLSKLL
                     ETVNLVSKRVNRELKVGGIVLCLYDAGTRHGGEVIEDLQTFFEGRRNADAPWAEAKVY
                     QTRIRRNIRLAECPSFGQSIFQYAPTSRGAEDYASLAAELRGLAPAEIWQGSDDKPTG
                     EKNASPDAASAA"
     gene            complement(5154..>5553)
                     /locus_tag="VHS97_14820"
     CDS             complement(5154..>5553)
                     /locus_tag="VHS97_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449525.1"
                     /translation="TDPGTTSIVFALNRGAGARLGPAFASRPGIIPDVLVTVTVGPYG
                     QSSSATITNLTTRTTSALPSPTIRIAGPTVRILIPASLLPTEGLSIKKYTFAVWTETQ
                     PGAGIQDVGSFVPENAMIPIGVETNVKPSL"
BASE COUNT         1119 a         1668 c         1689 g         1077 t
ORIGIN      
        1 ttgaagttgt aacgcgccgg cacggtcaac tgggcaggca ggtaggtccg ctcgccgttg
       61 ggcccggagc cggtctgcca gccgatttgc atgccgttgg gatcgaggaa ataaatctgg
      121 ctcttggtga cggtgaagcg ccgcccgttg tgggcgcccg gaccatttgg cccggccagg
      181 gaggccgcgg tatagggcgg agcgcccgtg gcgtcggtcg cagtcacggc atgagggcga
      241 agccacttct tgaggtggac cccagggtcg ttgtatccga atccgtcgcc ggcggtcgcc
      301 gccgccagca gcagcattcc gaacgcttgc atataatcct ccaactccct tctcgccgag
      361 gaacccggcc ggtcctgtcg tgaccctagc gcccgattca ccgaaccagg gagattgttt
      421 ctgcttcctg cttaaaacgg cgaccgcctc gaccgaggtc gagtcaatag tctccggacc
      481 gtctgccggc cttgcgtctc ctgacgccta gcgattatga tcgggcattc cttcccaaac
      541 ccttgaggaa ttgccctaca tcccaaccat gcttaataat cggactatca ccatccgttc
      601 gtgggcgatg cgcttggcgc ttgttgtgat cgtcgcctgc tgtttgcttt cgcaatcgct
      661 ggcccaggtt ccgcaaatgc agcccggggc cgcgggcgcg gcaggcgcaa cggcgcccga
      721 tcagccggtc actcccggtg ctcctcctgc cgacaagcca gacgaaccgc ccaccaaggc
      781 cgagctcctt atcgatgacg cgatcataaa gatcgccaag ctcgagtcgg tcgcagccga
      841 tctcctccag gagatcaata tgctcaatca gaaatacacg atcacgggcc gctatttgaa
      901 ggcaccgaat acgcatgtct acctcaaact gagcgtctcc ggcctgcctg atacaagcgc
      961 caccagcctg caggtttgcg acggcgagac gctctgggac taccaggtcg ttcttgattc
     1021 gcagaattac cgcaagctca gcatcaagcc gatcctggaa cgcctgaatt cgccggagct
     1081 taatgagagg atcaaagatc aggcgctcac ttcgatggga ctggccggac cggaaacgct
     1141 tcttgtaggg ctacgtaaga cgctcaaatt tactcaaata gaagaagaag aggtcgacgg
     1201 caaaaagttg tggcgattac gtggaacctg gcgcactagg caaggactgg tcggccccga
     1261 cggccggccg atgggcgccg ccggtttttt acccccctac attccgatgg atggcacgct
     1321 ctatcttggc aaggacgaca gctggcccta caagctcgtt cttgtgggca gaaagccatc
     1381 ggcattattc gaaacccgtc gcctcggccc gtacggccgg ccaatcggcg ccaagagctc
     1441 gatcgaaaaa atcaatccca gcgaaatccg gctgacctat tcagacgtga agctcaacgc
     1501 caagatccgt ctcgatgaat ttgccttcca ggctccccca gcagcggtgg tcgatgacag
     1561 caccgaggcg atcgtcaaag gactcgatca ggcgatttcg atggagaccc agcggaaaaa
     1621 gagtgaggcc gcgaagaagg agggtcctgt gctcgatcgt tcgatcgaca ttccctcacc
     1681 gccggacttg aacgcaccgc gaccctagcc ggcaacaagc catttccgat gagtaaatcc
     1741 gaattccaga gattatagac ctgaacccac aatcgtggag acatagggcg acgggcgaag
     1801 atggagccga ccaaaccggg tacaacacct aagccgagca aggttgaggc gatcaagctg
     1861 gcgagcgagt atcttaagac atacgtcgcc gacgagttga ataatgggtc tacacatttc
     1921 acggaagacg cggcgaccgt actgaagttt cacgggtcgt atcagcaaga cgatcgcgac
     1981 ttacgcaagc aactgaagag agaaggcaag gatcaggccc accaattcat ggtgcgtgtc
     2041 cggatcgtgg gcggcaagct gaacgctctt cagtacctgg cgtgcgacca cttggctcaa
     2101 gccatcggca acggcagctt gcggatcacg acccgtcagg aatttcaact tcatggcgtg
     2161 ctcaagcacg acttgcaggc gacgatgcgc caaatcaacg aagcactgct gtcaaccttg
     2221 gcggcgtgtg gagacgtcga gcgcaacgtc ctatgctgtc cggcgcctct ttacgactcg
     2281 attcgcgacg acctgaacgc cgacgcgctg gcctgggcgg cacattgcgc gccccggtct
     2341 tcggcttact gggaaatctg gctcgacggt gaaaagatcg aaagtctgcc gccagacgcg
     2401 agtatcgagg ttcccacacc cggtgatgat ccggtcgaac cgatctacgg caagacctac
     2461 ttgccgcgca agttcaaaac ggcctttgcg ttaccggaag acaactgcac tgacattcac
     2521 gctaatgacc tcggttttct cggagtcgtc gtcgacggac ggctggccgg ttataacgta
     2581 ctggccgggg gcggcctggg cacgaccccg agtgcgcaga agacatttcc cttcctcgcc
     2641 cagccgctgt gctatgtcga ccgcggcgat gtgaaaaaga tcggcgaggc ggtgctgaaa
     2701 gtctttcgag acttcggcaa tcgttctgat cgtaagcgag cccgtctcaa gtacatcatt
     2761 cacgactggg gaatgccggc ctttcgtgcc aaggtcgagg agtacctcgg tcactcgctc
     2821 gccgatccca agccgatcaa ggtcacgggc gtggacgacc acctgggctg gcacccgcaa
     2881 ggtaatggca gactctttct gggtatcccg gtggagaatg gccggatcaa ggacgacggc
     2941 gactttcggc tagccagcgg cttgcgaacg tttttccgga aataccagac gtcggcgcga
     3001 ctaacctgtc agcagtcgat cctgctcgaa gatatcgagc cgagccgccg cggcgagatc
     3061 gagtgctggc tggaggaata cgggatcgcc gcggtcgagc agatatcgac ggttcggcgg
     3121 tggtcgatgg cctgcccggc actgccgacc tgcgggttgg cggtcaccga agccgaacgc
     3181 gccctgccgg gaattctcga ccagctcgag gtcgagctag cgcgactcgg tctggagaaa
     3241 gaacggttca cggtaaggat gacgggatgc cccaacggct gtgcccggcc ctacaattcc
     3301 gatgtgggac tggttggccg atcggcccac atcaataaag actgcacacc tggaccggga
     3361 acatatacga ttttcctggg aggtcgtact gtcggcgacc gcctcaatat tgagttcaag
     3421 gactatgtgc cttacgacca ggtcgttgcc gagctcatgc cagtctttag ccgctttaag
     3481 gccgagcgta acgacggcga gtcgtttggc gatttctgcg accgcatcgg cgtcctagag
     3541 ttggcccgtt cggcggcgcc cgagctcaac cccgttggct agaccaaccc gcagctcgtc
     3601 gggaaccctg tcatggagat cgacgagccg gctcgagccg atctgatcgc cgcggcccgc
     3661 caggcggcag gacaagctta ttgcccgtac agccgctttc gcgtcggggc ggccgttctc
     3721 accgatcgcg gcgagatttt ctccggttgt aatgtcgaga atgcttcgta cggcctgacg
     3781 atctgtgccg aacgcaatgc gattttccag gctgtggcgc gctcgcaagc gcctgtggtg
     3841 atccgggcgg ttgtggtctt tacaccgact gacctaccga cggccccctg cggcgcctgc
     3901 cggcaggtga tcaacgaatt cggaccggag gctttcgttc tctccgtctg cgacggagag
     3961 gaggtcatcg agattcgtct ttccgagctc cttccgcggg cgtttggacc ggctaatttg
     4021 gagccattgc ggaagactga gaacaccgcg agtcgttgac cggctccttc ccggtcgacg
     4081 ggatcgtctc tggccatttc gcctttgcgt attctggcgg aatggttaca cttccgccca
     4141 gaatcgctcg gtggacgaat tggaagtctc gctcacggtg cgcactggcc accggccacc
     4201 gatgcccggg tcgcacggtg ggtatcgccc accctgaccg gaaactggga gggaacccgg
     4261 atgcgacgga tcgcggtttt gaatcaaaaa ggtggagtcg gcaagaccac cacggctgtg
     4321 aacctggcgg ccgccctggc gatgggcggg cacaagacgc tggtgcttga cctcgacccg
     4381 caggcgcatg ccaccttgca tctcggactg ctgccaggcc ggtctgggcc atcgctttac
     4441 gaggtcttga ccgaagggct gccgctggcc agcgtgcgac ggtcagtgaa cccaaatctg
     4501 tcgatctgcg gcagccatat cgacctggcc ggcgccgagc tcgagctctt ggggactgtg
     4561 gggcgtgagg tcatcctccg cgaccagctc gcagctgaca ctgaatcgtt cgattatgtg
     4621 ctgatggatt gtccaccctc gttgagcgtg cttacgttga acgcgctgtg cgcggccaac
     4681 gaggttttga tccccctcca ggcacatttc ctggcacttc atggcttatc gaagcttctg
     4741 gagacggtaa acctggtctc caagcgggtc aatcgcgaac tcaaggtggg ggggatcgtc
     4801 ctctgccttt atgacgccgg gacccgccac ggcggcgaag tcatcgaaga tctgcaaact
     4861 tttttcgaag gccggcgcaa cgccgacgca ccctgggccg aggccaaggt ctatcagacg
     4921 cggatccgcc gcaatatccg tctggctgag tgtcccagct ttggccagtc gatcttccag
     4981 tatgccccga cgagtcgcgg cgccgaggac tacgcatcgc tggccgccga actccgcgga
     5041 ctggctccgg ccgaaatctg gcaggggtcg gacgacaaac cgaccggcga gaagaatgcg
     5101 tctcccgacg ccgcgtcagc ggcgtaatgt ggtgtgggcg gctgatatgt gactcagagg
     5161 gaaggcttca cgttggtttc cacgccgatc ggaatcatcg cattttccgg cacgaagcta
     5221 ccgacatcct ggatcccggc gcccggttga gtctcggtcc agactgcgaa cgtgtatttc
     5281 ttgatcgaca atccttcggt tggcaacaga ctggcgggaa tcaagatgcg caccgtcggg
     5341 ccagcaatgc gaattgttgg cgatgggagg gcgctcgtcg tcctcgtggt cagattggtg
     5401 atcgtcgccg aagacgattg accataagga ccgaccgtca cggtgacaag gacgtcggga
     5461 atgattccgg gccggctggc gaaggccggg ccgaggcggg cgcctgcccc tcgattcaag
     5521 gcgaagacga tcgatgtcgt tccgggatcc gtc
//
LOCUS       DATAQL010000852         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3752786, whole genome shotgun sequence.
ACCESSION   DATAQL010000852 DATAQL010000000
VERSION     DATAQL010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3752786"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VHS97_14825"
     CDS             <1..282
                     /locus_tag="VHS97_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449526.1"
                     /translation="ERFVERFSAEPEQAADQLRRVLTRTRRLGRNPENGAIAVLAIHG
                     DRALVAILQQATCLTVLTWNQFVPRLTEFGRTKVPRNWGRLLRRLSSDT"
     gene            complement(351..>2081)
                     /locus_tag="VHS97_14830"
     CDS             complement(351..>2081)
                     /locus_tag="VHS97_14830"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3449527.1"
                     /translation="AALVVFLYSLLFTSLISLFAVMIIPDNVRMSRYSGNLIGGLAMN
                     VVGPQWARLILNAVVVVVGSLILSGAVNTAIVGSNGVLNRVSEDGVLPDWFLKPHPRF
                     GTSSRLINLVVILQVVTILASQGNVLALGEAYAFGVVWSFVFMSMSMLVLRFKRPEPR
                     EYEVPFNIRFGRFDLPIGMTFIFLTLAIAAIANLFTKEVATITGVAFTATFFGVFWIS
                     EHLHHRRIGAAAKRHEHLEQFNEKQADRLSIQSLNLTKPYRKLVAIRSPYNLGMLERC
                     LAETDPETTEVVVMTASVLPPGSGDLKPMITEHDRQLLTAVVNLAEHAGKPVKPAIVP
                     TNEPFFAMTLTAKTIGAQELIMGPSNKYRPEDQLDLVALYWMKVCDAKPDPLSIRVLG
                     KDRDVRLDIAGGSQIPKTGAAAAEIAHLLSELRKSWHGVERLLLAYDGSPLSADFLDT
                     VVSFLDPAVAVTLLDVGEEDPEHDGTDPIEDAKMVVQRGAERASALGRHVEWRVARGD
                     PGREIVRAAVEGKFDAIFMSLRGVYRRGDTTAFASNTRYVLEHAPCRVILGFAPKSIP
                     NPTAAAKLES"
BASE COUNT          431 a          634 c          631 g          385 t
ORIGIN      
        1 gaacgcttcg tggaacgatt ctcggctgaa cccgaacaag cggcggacca actccggcgc
       61 gtattgaccc gaacgcgccg tctggggcga aaccccgaaa atggggcgat tgccgtcttg
      121 gcgattcacg gcgaccgggc cctcgtggcc attcttcagc aggcgacctg cttgaccgtc
      181 ttgacgtgga atcagttcgt gccgcgactt acagagtttg gacgaaccaa agtcccacgc
      241 aactggggac ggctactgcg ccggctttcc agcgatacct gaacaccgat tcgtgcggta
      301 tgatcagggt cgacgagcct gtcgaaacac cctctggcgc ccgatcatca tcacgactcg
      361 agcttggccg ccgcggtcgg gttggggatc gatttggggg cgaacccgag gatcacacgg
      421 cagggggcgt gctcgagcac gtaccgggtg ttggaggcga acgccgtggt gtcgccgcgg
      481 cgatagacgc cgcggaggct catgaagatc gcgtcaaatt ttccctcgac ggccgccctg
      541 acgatttcac ggcccggatc gccccgggca accctccact cgacgtgccg tcccaaagca
      601 cttgcgcgct cggcgccgcg ctggacgacc atcttggcgt cttcgatcgg gtcggtcccg
      661 tcgtgttccg gatcttcttc gccgacatcg agcagggtca cggcaacggc tgggtcgagg
      721 aagctcacga ccgtgtcgag aaaatcggcg cttaatggac tgccatcgta ggccaggagc
      781 agccgctcga ccccatgcca gcttttgcgc agctctgaga ggagatgagc gatctcggcg
      841 gcggcggccc cggtcttggg gatctggctg ccccccgcga tgtcgagtcg tacgtcacgg
      901 tccttgccca aaacccgaat cgacagcggg tcgggcttgg cgtcgcatac cttcatccag
      961 tagagcgcga ccaagtcgag ctggtcttcc ggccgatact tgttcgacgg ccccatgatg
     1021 agctcttggg caccgatcgt cttggcggtg agggtcatcg caaagaacgg ctcgttggtc
     1081 ggcacgatcg ctggcttgac cggcttaccg gcatgctccg ccaggttgac taccgccgtc
     1141 aaaagctggc ggtcgtgctc ggtgatcatc ggcttcaaat cccccgagcc gggtggaagg
     1201 accgacgcgg tcatcaccac gacctcggtc gtctcgggat cggtctcggc caggcaccgt
     1261 tcgagcattc ccagattgta tggcgatcgg atcgccacca gcttccggta cggcttagtg
     1321 agattcaggc tctggatgct aagccggtcg gcttgcttct cattgaactg ctcgagatgt
     1381 tcgtgacgtt tggcggccgc accaatgcgg cggtggtgga gatgctcgct tatccagaaa
     1441 acaccaaaaa aggtcgccgt gaaggcgacg ccggtaattg tggcaacttc cttggtgaac
     1501 aggttggcga tcgccgcaat cgccagagtc aggaagatga atgtcatgcc tattggcaga
     1561 tcgaagcggc cgaaccggat attgaacggc acctcgtatt cgcgcggttc gggccgcttg
     1621 aagcgaagga ccagcatcga catcgacatg aaaacaaagc tccagacgac gccgaaggca
     1681 taggcttcac ccagcgccag cacgttcccc tggctcgcga ggatcgtcac gacctgaagg
     1741 atgaccacca ggttgatcaa ccgcgagctc gtcccaaatc ggggatgcgg cttcaaaaac
     1801 cagtcaggca agacgccatc ctcggaaaca cggttcaaca cgccgttgga gccgacgatc
     1861 gctgtattga ccgcacccga gaggatcagc gagcccacga ctacaaccac ggcgttcaag
     1921 atcaatcgcg cccactgcgg cccgacgaca ttcattgcca gaccgccgat cagattaccg
     1981 ctgtatcggg acattcgtac attgtccgga atgatcatca cggcaaataa acttatgaga
     2041 gacgtgaaca aaaggctata caaaaagacg acgagcgcgg c
//
LOCUS       DATAQL010000853         5245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4057868, whole genome shotgun sequence.
ACCESSION   DATAQL010000853 DATAQL010000000
VERSION     DATAQL010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4057868"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VHS97_14835"
     CDS             <1..563
                     /locus_tag="VHS97_14835"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3449528.1"
                     /translation="MGKQYERLDERLIDFVARQRVFFVGTAPTASEGHLNISPKGLDT
                     LRILGPTTVAYLDVTGSGIETVAHVRENGRITIMLCAFEGSPLILRLYGRGRVVEPGD
                     PEWEDLLARFPRQPGVRSVIIVELNRIADSCGFGVPEYEYRQERTQLTDYARRKGHTE
                     LARYRALKNCKSLDGLDGLARVEGGG"
     gene            653..823
                     /locus_tag="VHS97_14840"
     CDS             653..823
                     /locus_tag="VHS97_14840"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX3449529.1"
                     /translation="MRRLRVDGWVRVKSKGGHRQFKHPTKPGRVTVSGKASHTVPPGT
                     LASIRKQAGWED"
     gene            835..1050
                     /locus_tag="VHS97_14845"
     CDS             835..1050
                     /locus_tag="VHS97_14845"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX3449530.1"
                     /translation="MRRYAIVIENAGSNLAAYVPDLPGCIATAESKEDVGRLIREAIE
                     LHLEGLEEDGLPIPEPSSRVEYIEVNA"
     gene            complement(1091..2500)
                     /gene="asnS"
                     /locus_tag="VHS97_14850"
     CDS             complement(1091..2500)
                     /gene="asnS"
                     /locus_tag="VHS97_14850"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX3449531.1"
                     /translation="MTRLTIKDILAGKVDLGASVVIEGWIRTRRDSKAGLSFLQVHDG
                     SCFEPIQVVAEASVPNYQTEVLHLTTHCSVRVEGSLTESLGKGQRFEVKADRVEVVGW
                     VENPDSYPVSSKRHSMEHLRTVAHLRPRTNTFGAVARVRDCLSMAVHRYFHDHGFLWV
                     HTPIITTSDAEGAGAMFRVSTLDFANLPRDEHGSIDFSSDFFGKPASLTVSGQLNVES
                     YCLALSRVYTFGPTFRAENSNTTRHLAEFWMIEPEIAFADLNDDADLAEAFLKYLLRA
                     VLDERADDMAFFAQHIDKDCINRLETFVNATFERLDYGDAVKALERAVATGHKFEFPV
                     KWGMDLQSEHERYLTETLVGRPVVVMNYPKEIKAFYMRVNDDGKTVAAMDVLAPGIGE
                     LIGGSQREERLDVLDARLDEMGLPKEQYNWYRDLRRYGTVPHAGFGLGFERTIVYVTG
                     MVNIRDVIPFPRTPGNAEF"
     gene            complement(2735..>5245)
                     /locus_tag="VHS97_14855"
     CDS             complement(2735..>5245)
                     /locus_tag="VHS97_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3449532.1"
                     /translation="LLLGGSIISLPVYLAILAPARTITRHVIAISQMLIGALLIHLSG
                     GRMEMHFHIFGSLAILAFYNDWLVLVTASVVVALDHALRGVYWPLSVYGTLTSSPWRW
                     VEHVSWVIFEDIFLVRGCVQKVRQIRDTAVHQAEIEATRDKIEATVAARTDELRRANR
                     GLKRGIAEQQRVELELQRAKELAESANRTKSEFLANMSHEIRTPMNGIIGMTELVLDT
                     QMTRRQREYLGLVKSSAESLLTVINDVLDFSKIEAGRLSLDPISFLFRDALDDTLKTL
                     ALRAYSKELELACRIAPEIPEMMVGDASRLRQVLVNLIGNAIKFTEKGEVVLAVAVEE
                     RRKERVRLRFSVADTGIGIPSDKVHRIFEPFEQADGSTTRRFGGTGLGLAISAKLVEI
                     MGGQIWVDSQPGQGSTFWFTIEMGVDPIPVERSGRFDLDPERLEGLPILIVDDNATNR
                     KILSEILGNWNAQPVAVSCGRDAILQLRSASTRGEPFALALIDGMMPEMDGMDLARCI
                     RKEPTIAGTRLLLLTSAGRPDDSTVCRVLEIDACLTKPVRQSELFVAIMKVLAPTHAL
                     TDYRGLQTDARAQTLELPDHSGLRVLLAEDHPVNQMVAIRMLESLGHTVVVAPDGRQA
                     IDRLASGVFDLVLMDIQMPDMDGFEAVHAIRLSEAGSDRHMPVLALTAHAMQGDCDRC
                     LQAGFDGYLAKPIRRNDLKAALESIRASIPCNQKPRRGLLARLNVITAGDDKFACELA
                     ESFLEAAPGCLKSIEEALESADARRLVAEAHALKGISLTIGAEDLAASCQTLEVAGRR
                     ESLSDLDVEAARTRLVWNQVRSELESLTSREPAEVSTA"
BASE COUNT         1053 a         1563 c         1585 g         1044 t
ORIGIN      
        1 cgatgggaaa gcaatacgag cggttggatg agaggttgat cgatttcgta gcccggcagc
       61 gcgttttctt tgtcggcacg gcgcccactg cttccgaggg gcatctgaac atctcgccca
      121 aagggctgga cacccttcgc attctcggcc cgaccacggt ggcgtacctg gacgtcacgg
      181 gcagcggaat cgaaaccgtc gcccacgtgc gcgagaacgg acgcatcacg atcatgttat
      241 gcgccttcga gggatcgccc ttgattctgc ggctctacgg acgaggccgc gtggtcgaac
      301 cgggcgatcc tgagtgggaa gatcttctcg ccagattccc tcgccaaccg ggagtacgct
      361 cggtaatcat cgtcgagttg aatcggatcg ccgactcgtg cggatttgga gtgccggagt
      421 acgaatatcg gcaagagcgg acgcaactga ccgattacgc ccggcgcaag gggcacacag
      481 aactggcgcg gtatagggct cttaagaact gcaaaagcct tgacgggctg gacggtcttg
      541 cgcgcgttga gggcggcgga tgacgggccg ctcggcgact cgatctcaca attggccgtt
      601 gaccctcccc cgttagcgcg gatagcctca aaacacgaca gtcggggagg tattgcgacg
      661 gctacgagtc gacggctggg tgcgagtcaa atcgaagggc ggacaccgtc agttcaagca
      721 cccgacgaag cctggacggg tcaccgtgtc gggcaaggcc agtcacaccg ttccgccggg
      781 gactctcgcg agcatccgca agcaggccgg gtgggaagac tgatggagtg agtcatgaga
      841 cggtacgcaa tcgtgattga gaacgccggt tcgaatctgg ccgcatacgt cccggacctg
      901 ccgggttgca tagcgacggc cgagtccaag gaggacgtag gtcgcttgat tcgcgaggcc
      961 attgaactgc atctggaagg actggaagag gacgggttgc cgatccccga gccctcgagc
     1021 cgtgtcgagt acatcgaggt caacgcctga gccggcgtcg tggcggattc ggcccaccgc
     1081 tgtggcgtgt tcaaaactca gcgttacccg gcgttcgtgg gaacgggatc acgtcgcgga
     1141 tgttgaccat tccggtgacg tagacgatcg tccgctcgaa accgagcccg aacccggcgt
     1201 gcgggacggt accataacgc cgcagatcgc ggtaccagtt gtattgttcc ttgggcaatc
     1261 ccatctcatc gagccgggcg tcgagcacgt cgagccgttc ctcgcgctga gagccgccga
     1321 tcagctcgcc gattccgggt gccagcacgt ccatcgcggc taccgtcttg ccatcgtcat
     1381 tgaccctcat gtagaacgcc ttgatctctt tgggatagtt catgaccacc acgggccgtc
     1441 ccaccagcgt ctcggtcagg tagcgctcat gctcggactg gaggtccatg ccccatttca
     1501 ctggaaactc aaatttgtgc cccgtggcga ccgccctttc gagtgccttg acggcgtcgc
     1561 cgtagtcgag gcgctcgaag gtcgcgttga cgaaggtttc gagtctgttg atgcaatcct
     1621 tgtcgatgtg ctgggcgaaa aacgccatgt cgtcggcccg ctcatcgagc acggcgcgca
     1681 agagatactt gagaaaagct tcggcgaggt cggcgtcgtc gttgagatcg gcgaaggcga
     1741 tttcgggctc aatcatccag aactcggcca agtggcgggt ggtattggaa ttctcggcgc
     1801 gaaaagtggg cccgaaagta tagacgcggc tgagcgccag gcagtacgac tcgacgttaa
     1861 gctggccgga aacagtgagg ctggccggtt tgccgaagaa gtccgaggag aaatcgatgc
     1921 tcccgtgctc gtcccgaggc aaattggcga aatcgagcgt cgacacgcgg aacatcgcgc
     1981 cggccccctc ggcgtcgctc gtggtgatga tcggtgtgtg cacccaaaga aaaccatggt
     2041 cgtggaaata ccgatgcacg gccatcgaca ggcagtcgcg gacccgcgcg accgctccga
     2101 acgtgttggt ccgcggccgc aggtgtgcca cagtccgcag gtgctccatg ctgtggcgtt
     2161 tcgatgagac agggtagctg tctgggttct cgacccagcc gacaacctcc acgcgatcgg
     2221 ctttgacctc gaaccgctga cctttgccca aggattcggt caacgatccc tcgacgcgca
     2281 cggagcagtg cgtggtcagg tgcaggacct cggtctgata gttgggcacc gacgcctcgg
     2341 ccacaacctg gatcggctca aaacacgagc catcgtgtac ctgcaggaat gacagcccgg
     2401 ccttggaatc acgccgcgtg cggatccagc cctcgatcac gaccgatgcg cccaagtcca
     2461 ctttgccggc caagatatcc ttgattgtga gacgagtcat ggaatcctcc caggccttgg
     2521 ttcgcttttt cgatcctgac gagtgaacgg ccgaagctgg ttctcatgat aacccgccat
     2581 cgtcgcccga gcgattgccg agccgattcc ccgtcgattg ccgagccgat cgcccttttc
     2641 accgccccgg atttcgtccg agcgagctct gacaaaagca gagctcatcg gccgtgagtc
     2701 tcgcataacg atcgggcgcc gagaattcga cgcatcaggc cgtcgaaact tctgccggtt
     2761 cccgcgacgt gaggctttcc agttcactcc gaacctggtt ccaaacgagc ctggtccgcg
     2821 ccgcctcgac atccaagtcg ctcaagcttt cccgtcttcc tgcgacctcc agagtttgac
     2881 aggatgcggc gaggtcttcg gccccgatag tcaacgagat ccccttcaga gcgtgagctt
     2941 cggcaacgag tctgcgtgcg tcagcggact ccaaggcttc ctcgatgctc ttgagacagc
     3001 ctggtgcagc ttcgagaaac gattctgcga gttcgcacgc aaatttgtca tcgccggctg
     3061 tgatgacatt tagtcgggcg agcaagccgc gacgtggctt ttgattgcac gggatgctgg
     3121 ctcgtatgga ctcgagcgcg gccttgaggt cgttccggcg aatgggcttg gccaggtatc
     3181 cgtcgaatcc ggcctgcagg caacgatcgc agtccccctg catggcgtgt gccgtcagag
     3241 caaggaccgg catgtgccgg tcgctaccgg cctctgacaa acgaatggca tggacagcct
     3301 caaatccgtc catatcgggc atttgtatat ccatcaggac caggtcaaaa actcccgacg
     3361 cgagcctgtc gattgcctga cggccgtccg gcgcgacgac aaccgtatgc cccagacttt
     3421 cgagcatgcg gatggcaacc atctggttga ccggatgatc ttcggccagc aggacgcgaa
     3481 ggccgctgtg gtccgggagc tcgagagtct gcgccctcgc atcggtttgt aggccgcggt
     3541 aatccgtaag tgcatgggtt ggggccaaca ctttcatgat cgcgacaaag agctcggact
     3601 gacggactgg cttggtcaga caggcatcga tctcgaggac tcgacagacg gtggagtcat
     3661 cgggcctccc ggcggaggtt aagaggagca gccgagtgcc agcaatcgtc ggttcttttc
     3721 ggatgcatcg agccaggtcc attccatcca tttcgggcat cattccgtcg atcaaagcga
     3781 gggcaaatgg ctcgccccga gtcgaggcgg atcgcaattg gagaatcgca tcccggccac
     3841 atgagaccgc aaccggctga gcgttccagt tgccgaggat ctcactgagg atcttgcgat
     3901 tagtcgcgtt gtcgtccacg atcaggatgg gtaggccttc tagccgttcg ggatccaagt
     3961 cgaaccgtcc cgatcgctcg acggggatgg gatctacccc catttcgatc gtgaaccaga
     4021 aggtgcttcc ctggcccggc tggctgtcga cccagatttg gccgcccatg atctcgacga
     4081 gcttggcgga gatggccaga ccaagaccgg tgccgccgaa tcgtcgcgtg gtcgagccgt
     4141 cggcctgctc gaacggttca aaaatccgat gcactttgtc gctcggaata ccgatccccg
     4201 tgtcggcgac ggaaaaacgg agtcgtacgc gctcctttct tctctcctca acggccacgg
     4261 caagaacgac ctctcccttt tcggtgaatt tgatcgcgtt gccgatcaag ttgaccagga
     4321 cctgtcgaag tcggctcgcg tcacccacca tcatttcggg aatctcgggt gcaatccggc
     4381 aggccagctc cagctccttg ctgtacgccc gaagtgcaag cgttttgaga gtatcgtcca
     4441 gcgcatcgcg aaacagaaag gaaatcgggt caaggctcag cctgccagcc tcgatcttgg
     4501 aaaagtcgag gacgtcgttg atgaccgtca ggagcgattc cgccgaactc ttgaccagcc
     4561 ccagatattc gcgctggcgt cgcgtcatct gcgtatccaa aacgagctcg gtcattccga
     4621 tgatgccgtt catcggcgta cgaatttcat gactcatatt ggccagaaat tcgcttttgg
     4681 tccggttggc cgactcggcg agttccttgg ctcgctgcaa ttctaattcc acgcgctgtt
     4741 gttcggctat cccgcgtttc aaaccgcgat tcgcgcggcg gagttcatcg gttcgcgctg
     4801 ccacggtcgc ttcgattttg tcccgagtcg cttcgatttc agcctgatga acggcggtat
     4861 cgcggatctg acggaccttt tggacgcagc cgcgcaccag gaagatgtcc tcaaagataa
     4921 cccaactgac atgctcaacc catcgccagg gactggacgt cagcgtgcca tagaccgata
     4981 agggccagta cactcctctg agcgcgtgat ctagggccac cactactgag gcagttacca
     5041 gcaccagcca gtcgttgtaa aaggcgagaa tggctaacga tccgaagatg tggaaatgca
     5101 tttccatcct gccacccgag agatggatga ggagagcccc gattaacatc tgactgatcg
     5161 caatgacgtg ccgggtgatc gtcctggcgg gcgcaagaat cgccagatac accggcagac
     5221 tgatgattga accgcccagg agcaa
//
LOCUS       DATAQL010000854         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5126397, whole genome shotgun sequence.
ACCESSION   DATAQL010000854 DATAQL010000000
VERSION     DATAQL010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5126397"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VHS97_14860"
     CDS             complement(<1..768)
                     /locus_tag="VHS97_14860"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX3449533.1"
                     /translation="MADKKSGATVQKRPGGRTDNRSDREKNLREKPQLIADQLRLLIL
                     TGQVTEGESLGRELDLVEEFGVSRPSLREALRILETEGLITVVRGLRGGVVAREPDHR
                     ITARTASIVLQARNVALADVFDARVLIEPLAARQLAQSRNRQAAVKELRELVRQQEKV
                     INDPEAWGVTIGEFHEHLVARAGNQTLAIVAEMLNEVVARAIVAMTRTREQYGTTQAD
                     RRRGIRAHQKLIELIEAGQGPEAEEHWRTHMLLVAKQS"
     gene            complement(1020..1700)
                     /locus_tag="VHS97_14865"
     CDS             complement(1020..1700)
                     /locus_tag="VHS97_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449534.1"
                     /translation="MIILQQIHQVIGEREFDFEETFRDALLRPDFDSKVKLLWFAWSS
                     HGTGEGYEALSVIGFRDLSTWNELDERTRYGDLASWATAIDGMRYSLHSTLNRVPDWS
                     PTIHIELSGAGPKASPHEPSQLRLDTVSFGPDVDDGSKRVCDAAAESSGDLLELIGCW
                     IDYFGNAGSGLGHFLSRIRVSQALSDELDNEAVTARWAGSPSVLGRLPRSLIRSRFLR
                     TVPWSPLS"
BASE COUNT          340 a          550 c          529 g          389 t
ORIGIN      
        1 tgactgcttc gcgaccagca gcatgtgtgt gcgccaatgt tcttctgctt caggaccttg
       61 tccagcttca atgagctcaa tcagtttctg atgagctcgg attcctcggc gacggtctgc
      121 ttgagtcgtt ccatactgct ctcgggtccg ggtcatcgcc acaatggccc tggcaacgac
      181 ttcgttgagc atctcagcga cgatcgccaa tgtttggttt ccggctcgtg cgacgaggtg
      241 ctcgtggaac tctccgatgg tgacgcccca cgcttccgga tcgttgatga ccttttcttg
      301 ttgccgaacc agctctcgta gctccttcac agcggcttgc cgattgcggg attgggcaag
      361 ttgcctcgct gcgagcggct caatgagcac tcgggcgtcg aagacgtcag cgagcgccac
      421 gttacgtgct tggagcacga tggaggcagt ccgcgccgtg atccgatgat ctggctcgcg
      481 ggcgacgacg cctccgcgga ggccccggac cacggtgatg aggccctcag tttcaagtat
      541 tcggagtgcc tcgcggagcg agggccgcga aacgccaaat tcctccacca ggtccagttc
      601 acgaccgagg gactccccct cagtcacttg gcccgtcaaa atgagcaatc gcaactggtc
      661 ggcgatcagc tgcggctttt ctcgaaggtt tttttcccga tcggagcgat tatcggtacg
      721 gccacctggt cgtttttgca cagtcgcgcc gctcttcttg tctgccatca acgccctccg
      781 ggatgcctca tcatgctcgt cgcccctttt cggtgcagct actgggcggc gggaggctcc
      841 aggcggtggg acatacagcc ttcggctcct tcgggggttg tgcccccgcc ccacctggct
      901 gttcacgtag cagtctaacc agcaccttcg tggcggtcgg aatggttacg gctggtcaag
      961 aaattgtcgt cgttgagcca tggcgcctcc gctgagaggc gtggactcga gcgtaggact
     1021 catgagagcg gagaccaggg gactgtcctt aggaatcggc tcctgatgag gctacgcggc
     1081 agtcgtccaa gcacgctcgg cgacccggcc caacgcgcag tcacggcttc attgtccaat
     1141 tcgtctgaca aggcctgaga gacccgtatt cgcgagagaa agtggcccaa tcccgagccg
     1201 gcgttgccga aatagtcgat ccagcaacca ataagttcga ggaggtctcc cgatgattca
     1261 gccgccgcgt cgcagacgcg tttggagccg tcgtcgacgt ctggtccgaa ggagacggtg
     1321 tccaggcgca actgagaagg ctcgtgcggg ctcgccttcg ggccggcacc cgagagttca
     1381 atatggattg tgggcgacca atctgggact cggttcaggg tcgagtgaag ggaataacgc
     1441 atcccgtcta ttgcggttgc ccagctggca aggtcgccat agcgggttct ctcatcgagt
     1501 tcattccagg tcgagagatc gcgaaaccca atcacagaga gcgcctcata cccctcgccc
     1561 gtgccgtgcg atgaccacgc aaaccagagc aacttcactt tcgagtcgaa gtcaggtcga
     1621 agcagtgcat ctcggaacgt ctcttcgaaa tcgaattcgc gctcgccaat tacctggtga
     1681 atctgttgaa gaatgatcat tggaaccctc ccatcagtac accggcgacc aaggagcgca
     1741 ccgaacaagg cgcgttgtcc atcgatctcg cacgctaagc gcatcgtgca tccattcccc
     1801 tgcttctg
//
LOCUS       DATAQL010000855         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4057926, whole genome shotgun sequence.
ACCESSION   DATAQL010000855 DATAQL010000000
VERSION     DATAQL010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4057926"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VHS97_14870"
     CDS             <1..548
                     /locus_tag="VHS97_14870"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX3449535.1"
                     /translation="AEHRAKNLLANVGAMVQLSEADTPDGLKKAIAGRIAALAGIHSL
                     FGQSRGTGAELGSLVRQELAPYSRDERMRTQIEGPTVVLKPDLAQAIAVALHELATNA
                     AKYGALSVPEGQIRVEWSRATDGRVVLRWTEAGGPLVSPPTRKGFGTHVMEAMIRGHV
                     GRHVRLDWRAEGLACGITLPI"
     gene            1782..>2009
                     /locus_tag="VHS97_14875"
     CDS             1782..>2009
                     /locus_tag="VHS97_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX3449536.1"
                     /translation="MPGRHVTSSQTRVYLIAHQTDAPAIAAAKAGISRATAYRIESDP
                     RLPSRKTKPRGRRRPDPLASGWESEIVPMLEA"
BASE COUNT          410 a          552 c          635 g          412 t
ORIGIN      
        1 aggccgaaca tcgagccaaa aacctgttgg caaacgtggg ggcgatggtg cagctctcag
       61 aggctgacac acccgacggc ctgaagaaag cgatcgcggg acgcatcgct gcgcttgccg
      121 gtatccattc gttgttcggc cagtcgcgcg gaacaggagc cgagctaggt agtctggtca
      181 ggcaagaact agcgccttac tcccgagacg agaggatgcg gacgcagatt gaagggccga
      241 cagtcgtgct gaagccagac ctggcacagg ctatagcggt ggccctgcac gagttggcga
      301 ccaatgccgc gaagtatggt gcgttatccg tccccgaagg tcagattcgc gtcgagtggt
      361 cgcgcgcgac tgatggacgc gtcgtgctcc gctggaccga ggcgggaggt ccgctcgtca
      421 gcccgccgac gcgcaagggt tttggcaccc atgtgatgga ggcgatgata cgtggccatg
      481 tggggcgaca cgtgcggctc gactggcgcg ctgaaggtct cgcgtgcgga atcactcttc
      541 cgatctgacg tgctggataa acctcaggtc ggtgtgctga ggctcgtacc tgggcggaag
      601 cggtcgcatc accaacgatc acagttacgc cgtaaccaga atgaccaggt acacgaatga
      661 acgaccgggc cacattgagc gctatcgcgg gcgatagcag tcttgtcggt cagccttgat
      721 gatattgtgg ctcggtttgg catggagctc agagcaggga ggaggacgga cttggtcacc
      781 gcgcgaggcg tgctggaatt ggttgagcga cagcaccacc gctgaaccag cttgccaccg
      841 gcgcaacgct tatttgcaac gatattccag tagcgatgct ctttgccgtc cttgaagcgt
      901 cggttgaatc tcaggaaaaa tgatgcgaat catggagcgt tgaagtcgct ccattgaacg
      961 ccacctcgca tcatcggcta ggtagtaggt ctacactaca ggcgttttgc aggcgggcgc
     1021 cgttgattcg actcgcgttt ttgtggctgc gcctcgcgaa atttgccggt cgagcccccc
     1081 aatcggctaa gttgggttaa cgcagtcggg aaggcagccc gtgacccgat gcgcaaagtg
     1141 cgtgctcacg cgcagcaagt atgcaagtgc cctttttgta tgatgagatc ttaccggtcc
     1201 tcgagagtgc cactttctct gctgaccccg acgccgaaat ccctatgttt tgcgtccctt
     1261 gtgggatgac cgcgaatttc gtgatggttc cgaaattcga ggaatccgcg agaaattcgc
     1321 caagggggtt ggtaaaatgg ctgttggcgg cgagttccgc ccgcaacgcg ggttaacaag
     1381 aggctcttca agccgccatg gcgacgatcg attgatcatg tcgatcccag gcatcaagaa
     1441 cctcagcggg cacgtcatcg tctcagacga tcggcatcga catgagccgg tccccttcgg
     1501 ccgcacatct catctctggg cttgcgtatc tccgctttgg ggcgtcaatc aaggtgagac
     1561 tcgagcgaca atcaaggtga gacgagtgtc gggggtggta ccgacggttc ctggtcggga
     1621 atgaattcct ggtcaattgg aagcgtgggg tatcccatct caggcacggc tcgtgtttac
     1681 cataggcgga attaactccg ggttgtagca cgcttggtct ggctgtcgga gtggttgggc
     1741 gcagcatctg atccttctcc aacaaataga aggatcagcg tttgcctggc cgacatgtta
     1801 cctccagtca gacgagggtg tacttgattg ctcatcagac cgatgcccca gccatcgcgg
     1861 ccgcgaaggc gggcatttcc agagcgaccg catatcgcat cgaaagcgac ccgcggttac
     1921 catcacggaa gaccaaacct cgtgggcgcc gccggcccga cccgcttgcc agtgggtggg
     1981 agagcgagat cgtgccgatg ctcgaggct
//
LOCUS       DATAQL010000856         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2836006, whole genome shotgun sequence.
ACCESSION   DATAQL010000856 DATAQL010000000
VERSION     DATAQL010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836006"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VHS97_14880"
     CDS             complement(<1..775)
                     /locus_tag="VHS97_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449537.1"
                     /translation="MSERFYVDASPPGSDAFLQNVKGAGFSRFFIDRPIFAAVLSIVI
                     TLAGMLAVTALPIAQYPEITPPTVEVSAFYPGANAQVVADTVAAPIEQQVNGVENMLY
                     MSSQCTNDGTYTLTVTFKLGVDLNMAQVLVQNRESLADPILPDLVKRRGVAVKKKSPS
                     ILMIINLFSPDSSRDNLYMSNYATIQLKDELARLPGVGDITYIGQRDYSMRIWVDPQK
                     MAFRSLTAADVVSAITAQNAQVAAGRLGQPPVATGQVFQY"
     gene            complement(783..>3762)
                     /locus_tag="VHS97_14885"
     CDS             complement(783..>3762)
                     /locus_tag="VHS97_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449538.1"
                     /translation="YQLPGTNALDVADRVRAKMEELKTRFPDGVDYDIAYDTTPYVSE
                     SIYEVFKTLRDAVILVAIVVLLFLQNWRSAVIPLIAVPVAIVGTFAVMLAMGFSINNL
                     TLFGLVLAIGIVVDDAIVVVESVEHHIEHGMRPRAATIRAMDEVSAPVIAVGLVLSAV
                     FVPCAFITGITGQFFRQFALTIASSTIISTFNSLTLSPALAAILLKPKDKESHQPLPA
                     VAIAALGAWLGYARLGPALEHWLTKYDPAHLQKLGESINHGFAARQALWLSSHYPGPY
                     NQLVEAAPWLGTVAGALFILLMMKPLNWALAGFFSIFNRGFDLAGRAYTRIVGGLLRI
                     TVLVLLAYVGLLCLTGYGYVGFPKGVLSDEFIAKLDKGKEYEKFAELKPFLTFHGIPK
                     GFIPSHDMGYLMINVQLPDSASTERTLKTLRKMQDIALTTPGIGHSFMISGQSLLLSA
                     FGSNFCSVFLMLDDFSERPQPTTERFFDWYAQTGMEDSWRNRFGWPAKPDAKGANPAQ
                     APKTNLESWCRRTFGIKKVPSLNSDSIAGVLRRKFEEQIPEAMVMVLPPPPVRGVGRA
                     GGFKIMIEDRSSSAGSLEGLRELQKATENFVDEAKFETDPTTGSRRERPQFAQGMSSV
                     FRANVPQVFVDLNRSSAMTKDVPVQNVFQTMQIYLGSLYVNDFNLFGRTWQVIVQCEK
                     DFRDQVDDIQRLKVRNLHGQMVPLGSLADVREENGPFVLTRYNMYPASFINGAGAPGT
                     SSRQAIDLMDQVGADSLPPSMATEWTEMAYLELLAGNTAMVVFAFAVVMVFLVLAAQY
                     ESWSLPLAVILVVPMCLLSAITGVWIANMDINIFTQIGFVVLVGLASKNAILIVEFAK
                     VHREKGLSVREATLEACRLRLRPIVMTSIAFILGVVPLLLSSGAGAEMRRTLGTAVFS
                     GMLGVTAFGIFLTPVFFFVVEKLSETNLFASGPLRKISDLVLGILSFRAVRHLVRSKP
                     RPSVRPLSPVSEPGELVEHK"
BASE COUNT          811 a         1137 c         1116 g          698 t
ORIGIN      
        1 tgtactggaa gacctggcca gtggcgacgg gcggctgccc gagccgcccc gcggcgacct
       61 gagcattttg tgcggtgatg gcggaaacga cgtcggcggc cgtgaggctg cggaaggcca
      121 tcttctgggg atcaacccag atccgcatgc tgtagtcgcg ttggccaata tacgtaatgt
      181 cgccgacgcc cggcagccgc gccagctcgt ctttgagctg gatggtcgcg taattgctca
      241 tataaaggtt gtcgcggctg ctgtcgggcg agaacaggtt gatgatcatc aagatgctag
      301 gggacttttt cttgactgcc acaccgcgcc gcttgaccag gtcgggcaaa atcgggtcgg
      361 ccagcgactc gcggttctgc accaagacct gagccatgtt gaggtcgacg cctagtttga
      421 aggtaactgt cagcgtgtac gtgccgtcgt tagtgcactg cgaggacatg tagagcatgt
      481 tctcgacgcc gttgacctgc tgttcgatgg gcgcggccac cgtgtcggcc accacctggg
      541 cattggcgcc aggataaaag gcggacacct ccaccgtcgg cggcgtaatt tccggatact
      601 gggcgatcgg cagcgcggtg accgcgagca tgccggccag ggtgatcact atggacagta
      661 ccgctgcgaa gatggggcga tcgatgaaga atcgggaaaa gccagcgccc ttcacattct
      721 gaaggaacgc atctgaaccc ggcggagacg catcgacgta aaatcgttca gacacagcgg
      781 cctcacttgt gttcgaccag ctctccgggt tccgagaccg gcgagagggg acggacggat
      841 ggacgcggtt ttgagcggac caggtgccgg acggctctga agctcagaat ccccagcacg
      901 agatcactga tcttgcgcaa cggtcccgaa gcaaacaggt tcgtctcgct caatttctcg
      961 acgacaaaga aaaacacggg cgtaaggaag atcccgaagg cggtcacgcc gagcattcca
     1021 ctgaaaactg ccgtgcccag tgtacgccgc atttcggccc cggctccgct cgagagcaat
     1081 aggggcacga cgcccaggat gaaggcgatc gaagtcatca cgatcggccg caatcgcagc
     1141 cggcatgcct cgagggtggc ttcgcgcacc gacagcccct tctcccgatg caccttggcg
     1201 aattcgacga tcaaaatcgc gttcttgctc gccagcccca cgagcaccac gaagccgatc
     1261 tgggtgaata tattgatgtc catattcgcg atccagacgc cggtgatcgc gctcaagagg
     1321 cacatgggaa ccaccaggat cacggccaac ggcagcgacc agctttcgta ctgcgctgcc
     1381 agcacgagga acaccatcac gacggcgaac gcaaagacga ccatcgcggt attacccgcc
     1441 aggagctcga gataggccat ctcggtccat tccgtggcca tggacggcgg cagcgaatcg
     1501 gcgcctacct ggtccatcag gtcgatcgcc tgccgcgaac tcgtccctgg cgcgccagcg
     1561 ccgttgatga aagaggcggg atacatgttg taccgcgtga ggacgaacgg accgttttcc
     1621 tcgcgcacgt cggccagaga acccagaggg accatctggc catggagatt gcgaaccttc
     1681 aggcgctgga tgtcgtcgac ctgatcgcga aaatctttct cgcactgcac aatcacctgc
     1741 caggtcctgc cgaacaggtt gaagtcgttc acatagagcg agcctaagta gatttgcatc
     1801 gtctggaaga cgttctgcac cggcacatcc ttggtcatgg ccgagcttcg gttcaagtcg
     1861 acgaatacct gcgggacgtt ggcgcggaag accgaagaca ttccctgcgc gaactgaggc
     1921 cgctcgcgcc gactaccggt cgtcgggtcg gtctcgaatt tcgcctcgtc gacaaagttt
     1981 tctgtcgcct tctgcagctc gcgcagtccc tcgagcgagc cggccgacga actgcgatct
     2041 tcgatcatga tcttgaagcc gccggcccgt cccacgccgc gcactggggg cggcggcaag
     2101 accatcacca tagcctccgg gatctgctcc tcaaacttcc tccgcagcac accggcaatc
     2161 gagtcactgt tgagagatgg cactttcttg atgccgaaag tacgccggca ccaactttcg
     2221 agattggtct tgggcgcttg cgcaggattc gccccttttg cgtccggttt ggcaggccag
     2281 ccgaacctgt ttcgccacga gtcttccatc cctgtttggg cgtaccagtc aaagaaccgc
     2341 tcggtggtcg gctgcggacg ctcggagaaa tcgtcgagca tcaagaatac cgagcaaaag
     2401 ttggatccaa acgcgctcaa aagcagcgac tgccccgata tcataaacga atgcccgata
     2461 ccaggcgtag tgagggcgat atcctgcatc tttcgcaagg tcttgagcgt gcgctcggtc
     2521 gaagccgagt cgggcagttg cacgttgatc atcagatatc ccatgtcgtg agacgggatg
     2581 aatcccttgg gaatcccgtg gaacgtcagg aatggcttca attccgcgaa tttctcatac
     2641 tccttgccct tgtcgagctt ggcaatgaac tcgtccgaaa ggaccccctt gggaaatccc
     2701 acgtaaccgt atcccgtcag gcaaagcagg ccaacgtagg cgagcagcac caataccgtg
     2761 atccgcaaga gacccccgac gatccgcgtg tacgccctcc cagccaggtc aaatccacgg
     2821 ttaaaaatcg agaagaaccc ggccagtgcc cagttgagcg gcttcatcat gagcaggatg
     2881 aagagcgccc ccgctaccgt tcctagccat ggagccgctt ccacgagctg gttatacggg
     2941 ccgggatagt gactcgacag ccagagtgct tgccgtgccg caaacccgtg attgatgctc
     3001 tcgccaagtt tctgcaagtg ggcggggtcg tattttgtca gccagtgctc cagggcagga
     3061 ccgagccggg catatcccaa ccacgccccc agagcggcga tcgccacggc cggcagcggc
     3121 tgatgtgatt ctttgtcctt gggcttcaag aggattgcgg ccagggccgg gctcagggtc
     3181 aaggagttaa aggtcgagat gatcgtcgac gaagcaatgg ttagcgcgaa ctggcgaaaa
     3241 aactgtcccg tgatcccggt gatgaaagcg cacggtacaa acacggcgga cagaaccaag
     3301 ccaaccgcga tcacgggggc cgaaacctcg tccattgccc ggattgtcgc ggcgcgcggc
     3361 ctcattccgt gttcgatgtg atgctcgacg gactcgacga ccacgatcgc atcgtcgacc
     3421 acgatcccga ttgccagcac caagccgaag agggtcagat tattgatgct gaaacccatc
     3481 gccaacatca cggcaaacgt gcccacgatc gccacgggca cggcaatcag gggaatcacc
     3541 gccgaccgcc agttctgcag gaagagcagc acgacgatgg cgaccaagat caccgcgtca
     3601 cgcagcgtct tgaagacctc gtagatcgac tcgctgacgt acggagtcgt gtcgtaggcg
     3661 atgtcgtagt ccaccccgtc aggaaagcgg gtcttgagct cttccatctt ggctcgcacc
     3721 cggtcggcga cgtcgagcgc attcgttccg ggaagctggt ac
//
LOCUS       DATAQL010000857         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1310196, whole genome shotgun sequence.
ACCESSION   DATAQL010000857 DATAQL010000000
VERSION     DATAQL010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310196"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VHS97_14890"
     CDS             <1..349
                     /locus_tag="VHS97_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449539.1"
                     /translation="APFAGCRPRPARDYSIHAGYINELSFCDSPSYRQSLELRRYDVL
                     APKDAAAARQALLEIVDLATEQLQAKMDMLCQIAELDAASAADRASWDDTPEGERLRR
                     YEVTCQYNYIRTK"
     gene            658..1062
                     /locus_tag="VHS97_14895"
     CDS             658..1062
                     /locus_tag="VHS97_14895"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3449540.1"
                     /translation="MARTSIDRLGSLQKSVMEAVWELGEATVQQVRDRVDREPLPAYT
                     TILSVMQKLEKAGWLTHRPDGRSYIYQPIRSREEAGATSLRTFIERVFRGDRLLLFQH
                     LLDDENLSEADLKTLKTMIDRRRKDKKERHDV"
     gene            1055..>3993
                     /locus_tag="VHS97_14900"
     CDS             1055..>3993
                     /locus_tag="VHS97_14900"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3449541.1"
                     /translation="MFDFISIQPGASLVRDIIWQSSLFLAAGLAASFAFARRPARAYR
                     ILLLAIVGALVTPLFGHGVRRAGWGFWVTSPERTARPAASTLAADSSQAVLLAGRGSR
                     ATTPVELPAPNLAEPMTGRPRALATIDERGVGWQRVGKARLHLRNLLGPIGSATYPNI
                     LAGLWCMASGLCLGRLILSLLAGRRLVACAKPVTCEPIDRAAEAAGLRLGLRHRPDLR
                     ASSLVSCPAIWCWGRRPVIVLPAERELTTSLDWVGVFCHELAHWIRGDQWSSLLAEML
                     VCALPWHPLTWWTRQRLGRLAELACDDWVLAAGLPATDYAETLLRLVPSRRAALALTA
                     VSSRYGVFGRVRHILDEGRCSPVVGRRWACSSGMIMVLAASAVAMAQIRSVPSTEQSL
                     SGEAKTQTSNESSTTKVSSKEAAMRRTIRGSVLGPDGKPATGATVYWIGQRKPPLSYV
                     ALPKDQESSRSPQTEILARSDADTKGAFSLSADYDPDRYQLYNGWDVTLLAKAPGAGM
                     LSQPVKVDAGEITLRLAPEEVIHGRLLTPGGMPASDVRVTVNGFRNDRTQEGMFVGLT
                     PKDEEILACWLQPRRTDADGRFTLEGVPRGNYASLTFWHPEYAVDEVMVNTTVDGSLT
                     PVLRAFEITPVKPTFTHTLEPARPVQGRVTDKETGKPLAGLLVEMIPMRSHGGMPFHA
                     RTDATGSFRISGHGGARFYITSVYPAADLGYLAASDTASNWPAGAKFLEKNFALEKGR
                     IVRGRVIDADTKQPVAGAAVVYQPTRGNPNNRNYDLRNTVVTDSDGRFGITALPGQGF
                     LAVETPDEGYMRVRFEESWRGTVFPQGLASIDVPKGGEPKPVEITARKGITLEAKAIG
                     PDGKVVTDLVGFCEGIDAKLIDIWNQGHIFADGVFRLPGADPSKTYRVHLIQPEREIA
                     TVVDLKAEPQARKPIEVKLETCSSVHGKVVTTAGSPMQAGRVYPSLVIREKEGEMSRN
                     DVYRNTQ"
BASE COUNT          780 a         1176 c         1267 g          770 t
ORIGIN      
        1 tgccccattt gcgggatgca gaccccgccc cgcgcgtgac tattcgattc acgcgggata
       61 catcaatgag ctgtcatttt gcgactcccc gagttatcgg cagtcgctcg aattgcgtcg
      121 ctacgacgtt ctggcaccta aggacgccgc ggccgctcgg caggcgctct tggagatcgt
      181 ggatctggcc actgagcagt tgcaagcaaa gatggacatg ctttgccaga tcgccgaact
      241 cgacgccgcg tcggcggccg accgtgcgtc atgggacgac actccggagg gcgagcggct
      301 ccggcggtac gaagtgactt gtcaatataa ttacatcaga acaaaataat ggccacaacg
      361 ggttcagaat cgacaagccg cacctcgact gcaaagtcgg ccgtgccggc ataaccatcg
      421 atgaaacgtt cagttcagcg cgatcgcggc tcatgaggca gcggccctcg acccttctgg
      481 acccgtcgcc gatcttcggc agtgactcga cgtcgagccc ggtgatctga aaggccgacg
      541 gtcactattc ggacatgact attgacatcg gcctcctttt gactactatg gactgacgta
      601 ctatgcaaag tagtacgacg tctgtcgaat acacttcatg ttctggaggc aaagggaatg
      661 gcccgcacgt cgatagacag actgggctcg ctgcaaaagt cggtcatgga ggccgtctgg
      721 gagctgggtg aggcgaccgt gcagcaggtg cgcgaccggg tcgatcggga gccgttgccg
      781 gcatatacga cgatcttgtc cgtaatgcaa aagctggaaa aggcgggttg gctgacacat
      841 cgccccgacg ggcgtagcta tatctaccag ccgatccgat ctcgtgaaga ggctggtgcg
      901 acatcgctcc gcacgtttat cgaacgggtc tttcggggcg acaggctgct cttgttccag
      961 cacctactcg acgacgaaaa cctgagcgaa gcagacctga agaccttgaa gaccatgatc
     1021 gaccggcggc gaaaggacaa aaaggagcgg cacgatgttt gactttatct cgattcaacc
     1081 cggtgcttcc ttggtccgtg acattatatg gcaatccagc ctgtttctcg cggcaggctt
     1141 ggcggcgagc ttcgcgtttg ccaggcggcc agcgcgtgcg taccgcatct tgttgctggc
     1201 gatcgtgggc gccttggtca cgccgctttt cgggcatggg gtccgtcgcg cgggctgggg
     1261 attctgggtg acgagtccag agcgtaccgc tcggcccgcc gcgtcaactt tggccgcgga
     1321 ctcgtcgcag gctgtcttgc ttgcgggcag aggcagccgc gccacgacgc ccgtcgaatt
     1381 gccagcacct aacttggctg aaccgatgac cgggagacct cgcgcgctag cgaccatcga
     1441 tgagcgtggc gtagggtggc agcgcgtcgg aaaagcccgt cttcacttgc ggaatctttt
     1501 gggaccaatt ggatcggcaa cttacccaaa tattttggca gggctttggt gcatggcgag
     1561 cggtctttgc ctcgggcggc tgatcctttc gctgcttgcg gggcgccggc ttgttgcctg
     1621 tgccaagcct gtcacttgcg agccgatcga tcgagccgcc gaagccgctg gcctgcggct
     1681 cggtctgcgg catcgcccgg acctccgtgc ttcgtcgctt gtctcgtgtc cggcgatttg
     1741 gtgctggggc cgccggccgg tgatcgtatt gccagcagaa cgggaattga cgacatcgtt
     1801 ggactgggtc ggcgttttct gccacgagct ggcgcactgg atccgtggcg atcagtggtc
     1861 gagcctgctg gcggaaatgc tggtgtgtgc cctgccctgg catccgctga catggtggac
     1921 tcggcagcga ttgggacgtc tggccgagct cgcatgcgac gactgggtgc tggcggcggg
     1981 gctaccggcg acggattacg ccgagacact tctgcgtctg gtcccctcgc ggcgtgcggc
     2041 cctggcgctg acggcagtat cgagccggta cggcgtattc ggcagggttc gtcacatcct
     2101 ggatgaaggg cggtgcagtc cggtcgtggg tcgtcgctgg gcttgctcga gcggcatgat
     2161 aatggtgttg gccgcatcgg ccgtcgccat ggctcaaatc cggtcggtgc catcaaccga
     2221 gcagagtttg tctggggagg ccaaaacgca gacgtcgaac gagtcaagca caacaaaagt
     2281 aagctcgaag gaggccgcga tgagacgcac gatccgaggc tcggtgctcg gtcctgacgg
     2341 caaaccggcc acgggagcca cagtctactg gatcggccag cgcaagcctc ctctgtccta
     2401 tgtggcgttg cccaaggacc aggagtcgag tcgatcgcct cagacggaga tcttggccag
     2461 gtcagacgcc gacaccaagg gtgcgttttc cctgagcgcc gattacgacc cggaccgcta
     2521 tcaactctac aatggctggg acgtgaccct gctggcaaaa gcaccgggtg ccggcatgct
     2581 ttcgcagccg gtcaaggtgg acgcggggga gatcacgctc aggctcgctc ccgaggaagt
     2641 gattcatgga cgcctcttga cccctggcgg catgcctgcc tcggacgtgc gcgtgacagt
     2701 caacggcttt cgtaatgatc ggacgcagga ggggatgttt gtcgggctaa caccgaaaga
     2761 cgaagagatc ctcgcttgct ggctgcaacc tcgccggacg gatgcggacg gccgattcac
     2821 tctcgaaggc gtgcccagag gcaactacgc gtcactgact ttctggcatc ccgagtacgc
     2881 cgtcgatgag gtcatggtca acacgacagt ggatggttcg ctgacaccgg tgctgagagc
     2941 ctttgagatc acgccggtca aacccacgtt cacgcatacg cttgaacccg cgcggccggt
     3001 tcaggggcgc gtgacagaca aagagaccgg caagccgctg gccgggttgc ttgtcgaaat
     3061 gataccgatg aggagtcacg gcggcatgcc gtttcacgcc cgcaccgacg ccaccggcag
     3121 ctttcgcatt tcggggcacg gcggggcgag gttctatatc acatccgttt atccagcagc
     3181 agatttgggt tacctggccg ccagcgacac agcttccaac tggcccgccg gcgccaaatt
     3241 tcttgagaag aacttcgcgc tcgagaaggg ccgaatcgtg cgcggacggg tcatcgacgc
     3301 ggacaccaaa cagccggtcg ccggtgccgc cgtcgtttat caacccacac ggggcaatcc
     3361 taacaaccgc aattacgacc tgcgcaatac ggtcgtgacc gactcggacg gccgattcgg
     3421 catcacggcg ctgccgggcc agggtttcct cgccgtcgaa actccggacg aaggttacat
     3481 gcgcgttcga ttcgaggaga gctggcgggg caccgtcttt ccccagggtc tcgcatcgat
     3541 cgacgtgccc aagggagggg aacccaaacc tgtcgagatc actgcacgaa aagggataac
     3601 cctggaagcg aaagccatcg gcccggacgg caaggtcgtg accgacctgg tcggcttttg
     3661 cgagggaatc gacgccaagc ttattgacat ctggaaccag ggacacattt ttgccgatgg
     3721 cgttttccgt ctccctggcg cggatccctc gaaaacttat cgagttcact tgatccagcc
     3781 tgagcgcgag attgccaccg tggtcgatct caaagccgaa ccccaagcga ggaagccaat
     3841 cgaggtgaag ctggaaacgt gctccagcgt gcacggtaag gtggtcacaa ccgctggctc
     3901 gcccatgcaa gcggggcgag tctacccgtc tcttgtgatc cgtgagaagg aaggcgaaat
     3961 gagccggaac gacgtgtatc gcaacacgca aat
//
LOCUS       DATAQL010000858         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2686174, whole genome shotgun sequence.
ACCESSION   DATAQL010000858 DATAQL010000000
VERSION     DATAQL010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686174"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VHS97_14905"
     CDS             complement(<1..344)
                     /locus_tag="VHS97_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449542.1"
                     /translation="MRRFVLGWKAAGHDRTLRARIVNYADDFVICCRATAAEAMERMR
                     AMMARLKLTVNETKTRECRVPDETFDFLGYRFGRCYDRRTGRSYVGARPSPKKIHRLC
                     REISDLTSRSSSG"
     gene            743..2467
                     /locus_tag="VHS97_14910"
     CDS             743..2467
                     /locus_tag="VHS97_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3449543.1"
                     /translation="MTTPHLRVAIYARVSGEQQARDGTIASQLEAVTQRLASDGFECD
                     PELRFVDDGWTGKTLIRPGLERLRDQAAAGAIDRLYVLDPDRFSRKYAYQILMLEELT
                     RHGVEVVFLCNPLGQSPAENLLRQVQGMIAEYEEAKILERSRRGKRHAARQGSVSVLS
                     GAPYGYRYIGKHEGDGEARYQVVANEAQVVRKIFLWVGQDRCSIGEVCRRLKSEGIPT
                     RTGKMAWDRSVVWATLKNPAYKGTAAFGKTRSGPFKPQRLRPQRGRPQHPRRPVSWID
                     TASNDQVLIDVPAIVDEALFDAVHSQLEENRQRRRARPAGDRYLLQGLIVCKRCGYGC
                     YGKPTSPASSKKRIPYAYYRCTGSDAYRFGGERLCWNKQVRTDVLDAAVWEDVRRLLS
                     EPERIRVEYERRRQETTTDDGREVDQVRKLINQVKRNISRLIDAYEEGILEKSEFEPR
                     IGAARQRLSRLEEERVRKVAEESRESELRLVIGQLEEFARQVSEGLREPDWATQRKII
                     RALVKQVEIDEGEVRIVYRVSPSPFEGGPQQDGSQHCWGRDYPTLRRAAVGLVVAPIF
                     QILPWQVK"
     gene            complement(2475..3521)
                     /locus_tag="VHS97_14915"
     CDS             complement(2475..3521)
                     /locus_tag="VHS97_14915"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3449544.1"
                     /translation="MAISLPDARQLSDEVLEALRLRALRGCELGFSEADVADLLGVSR
                     ETVSRWWTAYAAGGLDAVPGDRTGRPVGSGRALSDEQARRIRDRIDEHSPGELGIAAA
                     VWSRKAVRDLIRQEFGIDMPVRTVGEYLKRWGYTAKVPRRHAKDQDPDEVDRWLEWTY
                     PAIEARAALEDAEIHWCDEAGARADQQPRKGYAREGDPARVEVPKPHIRMNLVATITN
                     EGEVHFLTYKESMTGALFVTFLEELLSETTRKVFLILDRLPAHEAKVVADWVAEHGDR
                     IELFWLPKYAQERNADEYLNNDLKGAIGAEGLPESQGELRSRIEWFMSKLLQLPEHVR
                     SYFRHPCVQYAAGD"
BASE COUNT          679 a         1201 c         1144 g          659 t
ORIGIN      
        1 cccgacgatg accggctggt gagatcgctg atctcccggc agagccggtg gatcttcttc
       61 ggcgacggac gggcccccac ataggaccgg cccgtccggc gatcgtagca gcggccgaag
      121 cggtagccca ggaagtcgaa cgtctcgtcc ggcacgcggc actcccgcgt cttggtctcg
      181 ttgaccgtca gcttcagccg cgccatcatg gcccgcatcc gctccatcgc ctccgcggcg
      241 gtggcccggc agcagatcac gaagtcgtcc gcatagttga cgatgcgagc cctcagcgtg
      301 cggtcgtggc ccgcggcctt ccacccgagc acaaagcgtc gcatgtagat attcgccaac
      361 aatggcgaga tcacgccccc aacaatgttg cgaaactctc gctgattccc gattctgtca
      421 tctccagaac gcggctccgg ccgaagtacg ggcaaggatg gaagccccga ctcgattccc
      481 gtccaaggca ggctgacagc cgcgctctcg gggaggagtc ggaatgatta catcttcacg
      541 gaggcgcgcg ctcgacgttg ccgttacccg tcaccacgaa gcctctcggc ttcaggagca
      601 atcgatcgct tgtgcatacg aggcattaat gcctgttatc tcctcgcgct cccgatgccc
      661 gcacgaacaa cggggcgatc tccccagggc cacgagtcga actgagcgtc cccgatctct
      721 tccagtggga gcttgaccgg ccatgaccac acctcatctt cgcgtggcca tctatgcccg
      781 ggtctccgga gagcaacagg cccgggatgg cacgatcgcg agtcaactcg aggcggtcac
      841 acaacggctc gccagtgatg gcttcgaatg tgatccagaa ctgcgattcg tggacgacgg
      901 ctggaccggt aagaccctga tccggccggg cctcgagcga ctccgcgacc aggctgccgc
      961 aggggccatc gatcggcttt acgtcctcga tcccgatcga ttttcacgca aatacgctta
     1021 tcagattttg atgcttgagg aactcacgcg tcacggcgtc gaggtcgtct tcctctgcaa
     1081 tcccctgggg cagagtcccg cggagaatct cctcagacaa gtccaaggca tgattgccga
     1141 gtacgaggaa gccaagatcc tcgagcgcag ccgccggggc aagcggcacg ccgcgcgtca
     1201 ggggtcggtc agtgtgctca gcggcgcacc ctacggatac cgatacatcg gcaaacacga
     1261 gggggacggc gaagcgcgct atcaggtcgt ggccaacgag gcccaagtgg tccggaagat
     1321 cttcctgtgg gtcggccagg accgatgttc catcggtgaa gtctgccgtc gcttgaaaag
     1381 cgaggggatc cccacacgga cggggaagat ggcctgggac cggtcggtgg tctgggcgac
     1441 tctcaagaat cccgcgtaca aggggacggc ggcctttggc aagacgcgtt cggggccgtt
     1501 caagccccag agactccggc cccagcgcgg tcgccctcag catccgagga ggccggtctc
     1561 ttggatcgat acagcatcga acgatcaggt cctcattgat gtcccggcga tcgtcgacga
     1621 agcccttttc gacgcggtcc actcccaact cgaggagaat cggcaacgcc gacgcgctcg
     1681 tcctgcggga gaccgatacc tgctgcaggg gctgatcgtc tgcaagcgat gcggctacgg
     1741 gtgctacggc aaacccacga gcccggcttc gtccaagaag cgaatcccct acgcctatta
     1801 ccggtgcacg ggctcggacg cgtatcgatt cggtggagag cggttatgct ggaacaagca
     1861 ggtccgtacc gacgtgctgg atgcagcggt ctgggaggac gttcgtcgtc tgctctccga
     1921 gcccgagcga atccgggtcg agtacgaacg ccgtcggcaa gagacaacga ccgatgacgg
     1981 tcgtgaggtg gaccaggtga ggaagctgat caaccaagtc aagaggaaca tttctcgact
     2041 gattgacgca tatgaagaag gcattctcga gaagtccgaa ttcgagccgc ggatcggcgc
     2101 ggcacggcaa cggctctctc gcctggagga ggagcgcgtc cgaaaggtgg ctgaggagag
     2161 ccgcgagtcg gaactgcggc tggtgatcgg ccagttggag gaattcgcga ggcaggtttc
     2221 cgaaggactc cgcgagccgg actgggcgac ccagaggaag atcatccgag ccctggtcaa
     2281 gcaggtcgag attgatgagg gcgaggtccg cattgtgtat cgggtcagcc catccccttt
     2341 tgagggtggc ccccaacaag atggttcgca acattgttgg gggcgtgatt accccacctt
     2401 gcggcgtgcc gctgtgggtc ttgttgtagc gccaatcttc cagatattac catggcaagt
     2461 aaaataatca caaatcaatc accggcggca tactgcacac agggatgtct gaagtaactc
     2521 cttacatgct ccgggagttg gagcaatttg ctcatgaacc actcgatccg cgaccgcagc
     2581 tccccctggc tctccggcag gccctccgcg ccgatcgccc ccttgaggtc gttattcagg
     2641 tactcgtcgg cgttccgctc ctgggcgtac ttcggcagcc agaacaactc gatccggtcc
     2701 ccatgctccg ccacccaatc cgcgaccacc ttcgcctcat gcgccggcag gcgatccagg
     2761 atcaggaaca ccttccgcgt cgtctcgctg agcaactcct ccaggaacgt aacgaacagg
     2821 gcccccgtca tcgactcctt gtaggtgagg aaatggacct ccccctcgtt ggtgatcgtc
     2881 gcgaccaggt tcatccggat gtggggcttc ggcacctcga cccgcgccgg atcaccctcg
     2941 cgggcgtagc ccttgcgcgg ctgctggtcg gcccgggcgc cggcctcgtc gcaccagtgg
     3001 atctcggcat cttcgagggc ggcccgcgcc tcgatcgccg gatacgtcca ctccagccac
     3061 cggtccacct cgtcggggtc ctggtccttg gcgtggcgcc gcggcacctt ggccgtgtag
     3121 ccccaccgct tgaggtactc gccgacggtc cgtaccggca tgtcgatccc gaactcctgg
     3181 cggatcaggt cgcgcaccgc cttgcgggac cacaccgcgg cggcgatgcc cagctcgccg
     3241 gggctgtgct catcgatccg atcccggatg cggcgggcct gctcgtccga gagcgcccgc
     3301 ccggagccga cggggcgccc ggtccgatcg ccggggacgg cgtccaggcc accggcggcg
     3361 taggccgtcc accaacggct gaccgtctcg cgcgataccc cgagcagatc ggcgacgtcg
     3421 gcctcgctga atcccagttc acaaccgcgc agggcgcgga gccgcagggc ctcgaggacc
     3481 tcatcggaca actggcgagc atccggcagg gagatggcca tggtcgtgtc ctccatcttc
     3541 cccatcctag cccgaacgcc cgcggttatg ccattgcttg agaatcttcg ttgccagtgt
     3601 aatagttctc tcagaatggc tctcacgcca gcttggtgag gcttgctcgc ttgctggatg
     3661 agcttttcga cgcgttccgt gcg
//
LOCUS       DATAQL010000859         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2227258, whole genome shotgun sequence.
ACCESSION   DATAQL010000859 DATAQL010000000
VERSION     DATAQL010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227258"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VHS97_14920"
     CDS             <1..296
                     /locus_tag="VHS97_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449545.1"
                     /translation="QTAKMITIIDPKTPNLDRTLRHRSGSGRLQYRRARAHGQPGVGW
                     CRVARNRGEAHRSAVGRGGPLDPADRSAPPYRFDPLAVNIVSQTRQINPAGLT"
     gene            complement(274..1626)
                     /locus_tag="VHS97_14925"
     CDS             complement(274..1626)
                     /locus_tag="VHS97_14925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449546.1"
                     /translation="MSQVNTASGDDESPPTVTPSLATEIGGPIDWGQTWARGSLGSLG
                     RFQLRERLGDGGFGQVFRAYDPRLDRDVAIKVLKKPNPTEKVMERFFREARAVARLDH
                     PYIVAVHDAGFDNNRCWVAYQLVSGRPLWWYRDHHQMDAMTVARIIRNLADALDHSHH
                     MGVVHRDIKPGNVLIDDQGRPRLIDFGLARRADFESDLTREGAIVGTPAYMSPEQALG
                     RSRQVDERSDVYSLGVIFHELLHGRRPDEPTVDAVAGRSSSPGGSPADMSFDLALPAD
                     SKIPVVLRRICAKATANDPALRHASARALVDELEVWLDQQRRAGERRSARVPGVTISL
                     AAAVLVSIGLVALYVSNKGRSGPDVDVPDHAATSGSPLIPISTLASAPRYGLELIGNR
                     EKHRYHRADCAYVPAMSERNRIPLQSPEDARAHGLEPCEKCRPPVAEYDRAVTSGQPG
                     "
     gene            2059..3486
                     /locus_tag="VHS97_14930"
     CDS             2059..3486
                     /locus_tag="VHS97_14930"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX3449547.1"
                     /translation="MSRINPLGALLSTALCFGAGISAVGADKSAVDFGRMAGSYRLER
                     DGWIYIHLEGTPDQIGYQHGSLLASEIADFLRVIKPYLEKSSKRDWNFYRDAAEKMLW
                     NAIDPQYQREIDGIVAGVVAKGVEADRWDLVALNANQELPYYYVPWLDKKEGKAPATH
                     APGNCSAFVASGSFTSDGKIVMGHNAWTNYVVGTRWNIIFDIKPTEGERILMDGLPGV
                     IASDDDFGVNSAGIMVTETTITQFEGWDPAGKPEFLRARKALQYSRSIDDFVHIMLEG
                     NNGGYANDWLIGDNKSGEIALFELGLKNHSVRRTRDGCFFGANFPDTEKLTREETKFD
                     TTKKDSSPNARKARWEGLIAEHKGKIGLDLAKAFETDDFDVITQQHGTNERTLCGRVE
                     ISPRGVPEWDWGPYYPGGTVQSKVIDGTLAGKMAFWGQIGHQGSDFIADQFLKEHSEY
                     DWARGLIKDLKCQPWSRFEVDIKDR"
     gene            3531..>3783
                     /locus_tag="VHS97_14935"
     CDS             3531..>3783
                     /locus_tag="VHS97_14935"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3449548.1"
                     /translation="MSTASAPTPDLLTAEEFAERPDPGYPEELVRGRIVPMPMPKPRH
                     GEICNRAGRIFGNCAEDRDLGRVLSNDTGVITERGPDTVR"
BASE COUNT          775 a         1160 c         1132 g          716 t
ORIGIN      
        1 cgcagacagc aaagatgatc accatcattg accccaagac accgaacctc gatcgtacgc
       61 tccggcaccg atctggttct ggacgcctac agtatcggcg tgctcgtgcg cacggtcaac
      121 ccggcgtagg gtggtgccga gtcgcgagaa atcgcggcga ggcccaccga tctgccgtgg
      181 gtcgcggtgg gcctctcgat cctgcggatc gttcggcacc accctaccgg ttcgacccct
      241 tggcggtcaa tattgtcagc cagacccgcc agatcaaccc ggctggcctg acgtgactgc
      301 ccggtcatat tcggcgacgg gagggcggca cttctcgcat ggctcgagcc cgtgggctct
      361 tgcgtcctcg gggctttgaa gcgggatcct gttccgctcc gacatcgccg gcacgtaagc
      421 gcaatcggca cgatgatagc ggtgtttctc gcgattgccg atgagttcaa ggccatatct
      481 tggggcggag gccagagttg aaatcggtat caagggcgag ccgctggtcg cagcatgatc
      541 gggtacgtca acatcgggtc ctgaccggcc tttgtttgag acgtagagcg cgacgagtcc
      601 gatcgaaacg agtaccgccg cggccaggct aattgtcacg cccggcacgc gcgccgagcg
      661 cctttcgccg gctcgccttt gctggtccag ccaaacttcg agctcgtcga ccagggcgcg
      721 ggccgacgcg tgtcgtaggg cggggtcatt ggccgtcgct ttcgcacaga tccgcctgag
      781 cacgaccgga atcttcgagt cagcgggcag ggccaggtcg aagctcatgt ccgccgggct
      841 tccgcccgga ctgctcgacc gacccgcaac ggcatcgact gttggttcgt cgggacggcg
      901 tccatgtaag agctcgtgaa agatgacacc caggctgtag acgtcactac gttcgtcgac
      961 ctgccggctg cggcccagag cctgctctgg gctcatatag gcgggcgttc ccacgatcgc
     1021 accctcgcgc gtcaggtcgg actcgaagtc ggcccgacgg gccaacccaa aatcaatgag
     1081 ccgcggccgc ccctggtcgt cgatcagcac gttgccgggc ttgatgtcgc ggtgaacaac
     1141 gcccatgtga tgggaatggt caagcgcgtc ggccaggtta cggataatcc gcgccaccgt
     1201 catcgcgtcc atctggtgat gatcgcgata ccaccacagc ggccggccgc tcacgagctg
     1261 ataggcaacc cagcatcggt tgttgtcaaa gccggcgtca tgaactgcca cgatgtaggg
     1321 atgatccagc cgcgccaccg ctcgggcttc tcggaaaaac cgctccatga ccttttcagt
     1381 gggattgggt ttcttgagca ccttgatggc gacgtcgcgg tcgagcctcg ggtcgtacgc
     1441 gcgaaaaacc tggccaaatc cgccatcgcc caggcgctca cgtagttgaa atcgaccaag
     1501 tgatccgagc gagcctcgag cccaggtctg tccccagtcg atcgggccgc cgatctcggt
     1561 cgccaacgaa ggggtcacag tcggcgggct ctcatcgtcc ccgctagcag tattgacctg
     1621 actcaaatgg ctctcgatcg gcccgtcgca atgaggacat tggttcgacg gggtccctgt
     1681 ggacgtagcg cttacctcca cgcttcgcag acagcccccg cacaccatcc tgaccaaagc
     1741 tttcatcacg cccactctcc acgcgaccgc gcacctggct gattttgccg aagttgcgcc
     1801 attttgctcg ctatagctta ttcgttagcg ccgggcttcc caaccaagaa tctacaacgt
     1861 ccaaccccgg gtgcgaactc gtccacttct atcccgccac aaacgccggc acccagagcg
     1921 tacagcggtg tgacaagtgc gcgtaagcaa gtcaggtcgg gtcgttttac gttgccgccg
     1981 gtccgcgtcg gcattaaagt tgctggatcg ccggcagacg cattccgaga tgtcataaat
     2041 tggaggtcga ctccgaccat gtcgcgcatc aatccgctgg gagctttatt atcgaccgca
     2101 ctttgctttg gcgcaggcat ttcggccgtg ggcgccgata agtcggcagt cgatttcggc
     2161 cgtatggcgg ggagctatcg tctcgagcgc gacggatgga tttacatcca tctggaaggt
     2221 actcccgacc agatcggcta tcagcacgga tcgttgctcg cgagcgagat cgccgatttt
     2281 ctgcgcgtga tcaagccgta cttggaaaaa tcgagcaagc gcgactggaa tttttatcgt
     2341 gacgcggctg agaaaatgct ctggaatgcg atcgatcctc aatatcagcg cgagatcgat
     2401 ggcattgtgg caggcgtagt cgccaagggg gtcgaggctg accgatggga cttagtcgcg
     2461 ctcaacgcca atcaggagtt gccctactac tacgtcccct ggctcgacaa gaaagaaggg
     2521 aaggcccccg ccactcatgc tcccggcaat tgcagcgcct ttgtcgcctc gggcagcttt
     2581 accagcgacg gcaagattgt catgggtcac aacgcttgga ccaactacgt cgttggcacg
     2641 cgctggaata tcatctttga tatcaaaccg acggagggcg agcgcattct catggatggc
     2701 ttgcccggcg tgatcgccag cgatgacgac ttcggagtca attcggccgg gatcatggtc
     2761 accgagacga ccatcacgca attcgagggc tgggatcctg ccggcaagcc cgagttcctc
     2821 cgggcgcgga aggcgctcca gtacagccgc tcgatcgacg acttcgtcca catcatgctc
     2881 gagggcaaca atggcggcta tgccaacgat tggctgatcg gcgataacaa gagcggcgag
     2941 atcgccctct tcgagctcgg tctcaaaaat cactcggtac ggcgaacccg cgacggctgt
     3001 ttttttggtg ccaactttcc cgacaccgaa aagttgacca gagaagagac caagttcgac
     3061 acgaccaaga aagactcatc gcccaacgct cgaaaggccc gctgggaagg actgatcgcc
     3121 gagcacaagg gaaagatcgg cctcgatctg gccaaggcct ttgagaccga cgacttcgac
     3181 gtgatcacgc agcaacacgg aaccaatgag cggactcttt gcggacgcgt cgagatctcc
     3241 ccgcgcggcg tgcccgagtg ggactgggga ccttattacc ccggtgggac agtgcagtcc
     3301 aaggtgatcg atggcacact cgccggcaag atggcgttct gggggcagat cggccaccaa
     3361 ggctcggact tcattgccga ccagttcctg aaggaacaca gcgagtacga ttgggcgcgc
     3421 ggactgatca aagacctgaa gtgccagccg tggagtcgat ttgaagtcga tattaaggat
     3481 cgataaatgc gataatcagt aaagtccaat taccaacgga ggaaggcacc atgtccacgg
     3541 catcggcgcc cacgcctgac ttgctgactg cggaagaatt cgccgaacgt cccgacccgg
     3601 gctatccgga ggaactcgtg cgcggcagga tcgtgccgat gcctatgccc aaaccgcgac
     3661 acggtgaaat ctgcaatcga gccggccgga tcttcggaaa ttgcgccgag gaccgcgacc
     3721 tgggtcgcgt gctcagtaat gacactggcg ttattaccga acgcggaccc gacacggtgc
     3781 gag
//
LOCUS       DATAQL010000860         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4669234, whole genome shotgun sequence.
ACCESSION   DATAQL010000860 DATAQL010000000
VERSION     DATAQL010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4669234"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VHS97_14940"
     CDS             <1..545
                     /locus_tag="VHS97_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3449549.1"
                     /translation="VRGLADASQKIGTVVALINDIASRTNLLALNATIEAARAGEAGK
                     GFSVVANEVKSLATQTAKATQEIGGQIAAMQQATSQAVTALRSISGTIQRMNEIASVI
                     AGSVETQGEATQSIAQAVQHAAAGTAEVNSNIAAVTHVVDETGNRAGHVLEAATEMTR
                     QASALKDEVANFLVAVQQAA"
     gene            complement(742..>1509)
                     /locus_tag="VHS97_14945"
     CDS             complement(742..>1509)
                     /locus_tag="VHS97_14945"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX3449550.1"
                     /translation="KSNELALSVVDGSLVCAPGKGGNWRLHARYDPARSRFVDLKLTT
                     LKEAERVDRTRIDAGRTIVSDRGYGRVRDFQAILTQGSDFITRVGWRAVRMRDAAGQP
                     LDVVAVLPADDTPLDQDVWVKGITRPLRLVIRRLPPEAAEKARRKRARKASKGGNKLD
                     PRTTKAAGYLILLTSLARNAQPAEQVVAIYPHRWQIEIGFKRLKTLGGLDELPAADPR
                     LARSWILAHLIAAVLTDDLANEIVGFPPSAGPIITQA"
BASE COUNT          280 a          471 c          502 g          256 t
ORIGIN      
        1 cggtgcgcgg ccttgccgat gcctcgcaga agatcggcac cgtggtggcg ctcatcaacg
       61 acatcgcctc gcggacaaac ctgctcgcgc tcaacgcgac gatcgaggcg gcgcgcgccg
      121 gcgaggcggg gaagggcttc agcgtcgtcg cgaacgaggt caagtcgctt gcaacgcaga
      181 ccgcgaaggc gacgcaggag atcggcgggc agatcgccgc catgcagcag gcgaccagtc
      241 aggcggtgac ggcgctgcgg tccatcagcg gcacgatcca gcggatgaac gagatcgcct
      301 cggtgatcgc cggatcggtg gagacccagg gcgaggcgac tcagtcgatc gctcaggcgg
      361 tgcagcacgc cgccgccggc accgccgagg tcaacagcaa catcgccgcc gttacacacg
      421 tggtggatga gacaggcaac cgagcaggtc acgtgctgga ggcagcaacg gagatgacga
      481 ggcaggcatc ggcactgaag gacgaggtcg cgaacttcct ggtcgcggtg cagcaggcgg
      541 cttgaggaag agcggggcgt tgccccatag gcgcgaagtt aaggtggtct gagcaagtga
      601 gcttgcagct ttcgtttgcg cggtggctct gagcggcgtc gacgaaagcg atcgagatcg
      661 acctttctgc acaacctcgg cttcggcatg atggcatgct tgagcagcgt ccgcgcctcg
      721 ctccaggccg cccatagcaa ttcaggcctg ggtgatgatt ggcccagcac tggggggaaa
      781 accgacaatc tcattggcga gatcatcggt cagaacggcg gcgatcagat gtgccagtat
      841 ccaactccga gccaatcgtg gatcggctgc gggcaattcg tccagccctc ccagggtttt
      901 cagccgtttg aaaccgattt cgatctgcca gcgatgggga tagatcgcca ccacctgctc
      961 agccggttgc gcgttgcgtg ccagcgaggt cagcaggatc agataaccgg ccgccttggt
     1021 cgtgcgtgga tcgagtttgt tgccgccctt gctggctttg cgtgcccgct tgcggcgggc
     1081 tttctccgcc gcttcgggtg gaagacgccg gatcaccagg cgcagcggcc gggtgatgcc
     1141 tttcacccac acatcctggt cgagcggcgt gtcgtcggcg ggtaaaaccg ccaccacgtc
     1201 gagcggttga ccagccgcat cgcgcatacg caccgcgcgc caccccaccc gggtgatgaa
     1261 gtcgctgccc tgcgtcagaa tggcctggaa gtcgcgcacc cgcccatagc cacggtccga
     1321 aacgatcgtc cgtccggcgt cgatgcgggt gcgatcaacc cgttccgcct ctttgagcgt
     1381 ggtgagtttc agatcgacaa aacggctcct ggcaggatcg tagcgggcat gcagccgcca
     1441 gttcccgcct ttgcctggtg cgcaaacaag cgagccgtcc accactgaca gcgccagttc
     1501 attgctttt
//
LOCUS       DATAQL010000861         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3602601, whole genome shotgun sequence.
ACCESSION   DATAQL010000861 DATAQL010000000
VERSION     DATAQL010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3602601"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..>1875)
                     /locus_tag="VHS97_14950"
     CDS             complement(38..>1875)
                     /locus_tag="VHS97_14950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX3449551.1"
                     /translation="TGEPVAGAKLQVHAGDIFEFPNPDHRIVESGADGLFAVDLPAGP
                     IQVQLAEPPPGYYWVPSRPGSVDSLAVGALEPVVHREYRVRKGTIWDFRLTRGPERKP
                     FLGFVSGNSALLHEMFQAHSDEAGQTHMALPSDAHDVVLYVRESSWEPSQLETGYLFL
                     RLKWESNFRPDELQQISPLEGKVRGFRLTDANEKWATLHAAAPIEPFNDNGRLLIRVA
                     VPGRDSKDFGAVTGQVLDEKGRPIAGARVGLAAARNRVSHELRHQSTTDAQGWFRLRD
                     IPRRTIDSEPSKFQIVVVKKGYAGFVSPPLTLKDGTTEKFEVVDPIRLEPGVTISGIV
                     VDHRGRSVAGARVRTNRPVPHAGLAENIPTVRADENGRFTMHDLQRGMTLLTAYDGIL
                     IATPRYFRVGSSQSALLQLPEPRFDRDAPKKAVGARSEPLRLGQAALEWQMGLWSDGR
                     AHNLADHRGKAIVLYFWGTDFWQSVAVLPALGKLAAQFEPRGVVFRTLHRPDGDEKRV
                     GEEVRRLFAAKQAPLVFAFDQVQIDHHSRGVTAHQYGVENYPVVILINQAGKIAFRSD
                     MAAGDRNVAAVFMKILTDPQSMTEEKANRLVQEAVAEEIEGVLKQ"
BASE COUNT          375 a          573 c          553 g          374 t
ORIGIN      
        1 ctcttttccc cggccaacgc atttgaagca ttcaatctca ctgcttcaaa accccctcga
       61 tctcttcggc gacggcctcc tggaccaaac ggttggcttt ctcctcggtc attgactgag
      121 gatctgttag aatcttcata aacacggcgg ccacgttacg gtcgccggcc gccatgtcgc
      181 ttcggaaggc gatctttccc gcttggttga tcaggatgac gacggggtaa ttttcgacac
      241 catactggtg tgcagtcacg ccacgggaat gatggtcgat ctgaacctgg tcgaacgcaa
      301 agacgagcgg cgcttgcttg gcagcgaata gcctccgtac ttcttccccg acgcgtttct
      361 cgtcgccatc cggtcggtgg agggtccgaa acacgacacc gcgcggttcg aattgggcgg
      421 cgagtttccc aagagctggg agcactgcga cactttgcca gaaatcggtc ccccagaaat
      481 agagcacgat ggctttaccc cgatgatcgg caagattgtg cgctcggccg tcggaccaga
      541 gtcccatttg ccattccagg gcggcttggc ccagccggag cggctcggat cgcgcgccaa
      601 cagccttctt aggggcgtcg cggtcaaatc tcggctccgg gagctgcagt aaggccgact
      661 gagaggatcc gactctgaaa tatctgggag ttgcgatcag aatgccgtcg tatgcggtta
      721 acagagtcat tccgcgctga aggtcgtgca tggtgaatcg accgttctcg tccgcgcgga
      781 ccgtcgggat attctcggcc aaaccggcgt gaggaacggg tctgttggtg cgcacgcggg
      841 caccggccac ggagcggcct cgatgatcaa ccacaatgcc cgagatcgta acgccgggct
      901 ccagtcgaat gggatcaaca acctcaaact tttccgtggt gccatctttg agcgtgagag
      961 gcggcgagac gaaaccggca tagcctttct tcaccacgac gatctgaaac ttcgaaggct
     1021 cactgtcgat tgtccggcga gggatatcgc gcaggcgaaa ccatccctga gcgtcggtcg
     1081 ttgattggtg ccggagctca tgcgagacgc gatttcttgc cgccgccagc ccgacgcgcg
     1141 cgcctgcgat cggccggccc ttctcgtcga gcacctggcc tgtcactgcc ccaaagtcct
     1201 tggaatctcg ccccgggacg gccacgcgga tcaagagcct gccattgtcg ttgaagggtt
     1261 cgatcggggc agccgcgtgc agcgtggccc acttttcgtt ggcatcagtc agacggaaac
     1321 cgcggacctt gccctccaga ggagagatct gctggagctc gtcgggccga aaattcgact
     1381 cccacttcaa gcgcagaaaa aggtaacccg tctcaagctg cgatggttcc caggatgact
     1441 cccgcacgta caaaacgacg tcgtgagcgt cgctcggcag cgccatgtgc gtttgaccgg
     1501 cttcatcgga gtgcgcctga aacatttcgt gcaaaagggc gctgttcccg gacacgaacc
     1561 cgagaaacgg ctttcgttcc ggaccccgtg tgagccgaaa gtcccagatg gtgccttttc
     1621 ttacgcgata ctccctgtga actaccggct caagggcccc gacggcgagc gaatcgaccg
     1681 atcccggacg gcttggaacc caatagtagc cgggaggggg ttcggcaagc tggacctgaa
     1741 tgggaccggc tggcagatcg acggcaaaca aaccatcggc accggattcg acgatcctgt
     1801 ggtccggatt cggaaactcg aagatatcac ccgcatggac ttgcagcttt gcccccgcga
     1861 ccggctctcc cgttc
//
LOCUS       DATAQL010000862         3273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1615200, whole genome shotgun sequence.
ACCESSION   DATAQL010000862 DATAQL010000000
VERSION     DATAQL010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615200"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VHS97_14955"
     CDS             <1..205
                     /locus_tag="VHS97_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3449552.1"
                     /translation="FVYRPAISTQEVRQRRLTSLVDKLYQGEPGLLVLHLLKSHPLEP
                     SQAKEVRAVLDQMGNEKSKKKDK"
     gene            522..1454
                     /locus_tag="VHS97_14960"
     CDS             522..1454
                     /locus_tag="VHS97_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449553.1"
                     /translation="MARRKRWYDPDATADVRVIPQSYGDGPINPLRWRHARVEPSLGE
                     CLIYPLKDGPGLGLLLFLPPILWLFSLPIFDIISVLQPLTKSDWALGLLVVPVLIPVL
                     FSFSMTFGYALLFLGHVLVSSALGEIDHPRWPEWHPADISEGICRWFWAGFFGLLLGG
                     IPVAYYWMHCGAIDWFDWIVFAELLIIGVAYAQMALAASLLHENLIAANPITVVMSIF
                     RLGGCYLRPCLVASATLVLAGLGIRGLLYHMPKMWMEAVALWAFWLVVLYCAMVALRM
                     MGLTYHAHALDLSWFKRRPKWASSRHHGQIYANS"
     gene            complement(1500..2879)
                     /gene="mgtE"
                     /locus_tag="VHS97_14965"
     CDS             complement(1500..2879)
                     /gene="mgtE"
                     /locus_tag="VHS97_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX3449554.1"
                     /translation="MRHPLLVPDLRELIRDGEAAALRDFFADHHPRRLAEIIEDLETD
                     DGDALFQILPPRSRADVFSYLDENRQLRLVQAMPPKDTAELLHLMSHDERADLVNRLG
                     EDVVDPVLPYLAQAEREDIRRLASYEPGTAGAAMTTDYVTLPPHITVREALELIRHEA
                     PDRETIYYCYVVDHKRRLIGFVSLKKLILARRAAVIEDIMQQDVIFARVNEDQEAVAR
                     QIDKYDLIAIPVVDASDMLVGIITHDDAMDILRQEQTEDMLAFGGVSRDAEADEETYW
                     QVHIVQGVRRRIFWLFFLFGAGFLTNYVSKRFQGVRLRFTDLVDFVPLMIGTGGNAGS
                     QTVGTIIRGLALGEIQGSQTVRVVLRECFTGLLLGLILGSFGFLWTWYLHSNPAFAAV
                     IGLAILFICIWANSMGAIVPLIAKRLGIDPALVSAPLISTLVDATGLVIFYGIAILIL
                     IRLMGPASP"
     gene            complement(3042..>3273)
                     /locus_tag="VHS97_14970"
     CDS             complement(3042..>3273)
                     /locus_tag="VHS97_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX3449555.1"
                     /translation="VTSTSDCTDYQARRLNIRYRPSGQKGTRFVHTLNGTAIALSRAL
                     IVVLENYQRSDGLIDVPLVLRPYVGKDVIGCR"
BASE COUNT          630 a          972 c         1012 g          659 t
ORIGIN      
        1 gtttgtttat cgcccggcaa tctccacgca ggaggttcgc cagcgcaggc tcaccagcct
       61 ggtcgacaag ctctatcaag gcgagcccgg gttactggtg ctgcacctgc tcaaatcgca
      121 cccgctcgag cccagccagg ccaaggaagt gcgcgccgtg ctggatcaga tgggcaacga
      181 gaaatcgaaa aagaaagata agtaatcggg gccgtggatc ctacgcggtc tgtccagttc
      241 actcggcacg tccccaaact ccccgcgact cggtcagtcg acccacaaac ctcgcacggc
      301 ccgcgccgcg caacctatgg ggccgctccc ccctggcttc gcggccctca tttcgcgata
      361 atgaaaccct tcggatcgaa cttccccaca gggtccatgc acgttgggga ggatccactg
      421 ggatcataac gaacgttcca accgggccgg tttccaggag agccgagcgg gtattctggc
      481 ccctggcgca gccggacggg ttgattccgg aatgagctgt catggccaga cgcaagcgtt
      541 ggtacgaccc cgacgcgacg gctgacgttc gcgtcattcc gcagtcgtac ggggacggac
      601 ccataaatcc gctccggtgg cggcacgccc gggtagaacc cagcctggga gaatgcctga
      661 tttatcccct gaaagacgga cccggactcg gtctattgtt gtttctcccg ccgattctct
      721 ggcttttctc gctaccgatt ttcgacatca tttccgtcct ccagccgctg accaagagcg
      781 attgggcgct tggcctcttg gttgtgcctg tgctgattcc cgtgcttttc agcttctcga
      841 tgacctttgg ctatgcactc ctgtttctgg gacacgtatt ggtttcgagt gccttgggcg
      901 agatcgatca cccccgctgg ccggaatggc atcccgccga catttcagaa gggatttgcc
      961 gctggttctg ggcgggcttt ttcggcctct tgctgggagg aatcccggtc gcgtactact
     1021 ggatgcactg cggggctatc gactggttcg attggatcgt cttcgccgag ctactgatca
     1081 tcggagtcgc ctatgcccag atggcgctgg cggcctcgct cttgcacgag aatctgatcg
     1141 cggccaaccc gatcacggtc gtgatgtcaa tctttcgcct cggaggttgc tatctgcgtc
     1201 cctgcctggt cgccagcgcg accctggtgc tggccgggct gggaatccgc ggcctgctct
     1261 accacatgcc caaaatgtgg atggaggccg ttgctctctg ggcgttttgg ctcgtggtgc
     1321 tctattgcgc gatggtcgcg ttacggatga tgggactgac atatcacgcc catgcgcttg
     1381 atctgtcatg gttcaaacgc cgcccgaaat gggcaagctc gcggcatcac ggtcagattt
     1441 acgcgaattc gtagggcggg ccgtccggcc cgcgatgcaa caggccgcgg tcgacgactt
     1501 cacgggctcg ccggtcccat cagcctgatc aagatcagga tcgcgatgcc gtaaaagatc
     1561 accagtccgg tggcgtcgac cagagtgctg atcaaggggg ccgagacaag tgcggggtcg
     1621 atgcccagac gcttggcgat caaaggcacg atcgcgccca tgctgtttgc ccagatgcag
     1681 atgaagagta tcgccagccc gatgacggcg gcaaacgcag gattgctatg cagataccat
     1741 gtccacaaaa aaccaaaact gccgagaatg agtcccaaaa gcaggcccgt gaagcactcg
     1801 cggaggacca cgcgaacggt ctgcgagccc tgaatctcac cgagcgccag gccacgaatg
     1861 atggtcccga ccgtctggct gccggcattg ccgcctgtac cgatcatgag cggaacgaag
     1921 tcgaccaggt cagtaaatcg gagccgcact ccctggaacc tctttgaaac gtagttcgtc
     1981 aaaaagcccg ccccaaagag gaaaaacagc cagaaaatcc ggcggcgaac tccctggacg
     2041 atgtgcacct gccagtaggt ttcctcgtcg gcttccgcat cgcgagagac gccaccgaat
     2101 gccagcatgt cctccgtttg ctcttgccgt aagatgtcca tcgcgtcgtc gtgcgtgatg
     2161 atgccgacga gcatgtccga agcgtcaacg accgggatcg cgatcaggtc gtacttgtca
     2221 atctggcgag cgacggcttc ctgatcttcg ttgacgcggg cgaagatgac gtcttgctgc
     2281 atgatatctt cgatcacggc cgcccggcgc gccaggatca gcttcttgag agagacaaaa
     2341 ccgatcagcc tgcgcttgtg gtcgacgacg taacagtagt agatcgtctc gcggtcggga
     2401 gcctcgtggc gaatgagctc gagtgcctcg cggacggtga tgtgcggggg cagggtgacg
     2461 tagtcggtgg tcatcgccgc gcccgccgtc cctggctcat aactggcgag ccggcggata
     2521 tcctctcgct cggcctgtgc cagatacggc agcacggggt cgaccacgtc ctcgcccagc
     2581 cggttgacga ggtccgcacg ctcgtcgtgg gacatcaggt ggagcagttc ggccgtgtct
     2641 ttggggggca tcgcctgaac aagccgcagc tggcggtttt cgtccaggta gctgaagacg
     2701 tcggcacgac tgcggggagg caatatctgg aagagggcat cgccgtcgtc cgtttcgaga
     2761 tcctcgatga tctcggcaag ccggcgagga tggtggtcgg caaaaaagtc acgcagggcg
     2821 gcggcttcgc cgtcgcggat taattcccgg aggtcgggta ccaggagcgg atgtcgcatg
     2881 gaccggcctc aggcacgtgg cacggagaca tttgaacgcg gtagaaatta ttcgcacagg
     2941 ccgattatgt gcctcgtttg gagcaatcgc cgacctttag gatccggcgg ggtcccaggc
     3001 gcgacaaaat cagacgaatg agattggtcg cgcacgaact gttatcggca gccgatcacg
     3061 tccttgccca catagggtcg caggactagg gggacgtcga tcaggccatc ggaccgctgg
     3121 tagttttcca acacgacgat caacgcgcgg ctaagggcga tggccgttcc gttgagcgtg
     3181 tggacgaagc gtgttccttt ttggcctgaa ggacgatagc ggatattgag cctgcgggcc
     3241 tggtagtcgg tgcagtccga agtgctggtg acc
//
LOCUS       DATAQL010000863         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4974697, whole genome shotgun sequence.
ACCESSION   DATAQL010000863 DATAQL010000000
VERSION     DATAQL010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4974697"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..>2334)
                     /locus_tag="VHS97_14975"
     CDS             complement(447..>2334)
                     /locus_tag="VHS97_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449556.1"
                     /translation="TTEPFVPQAKRSAVVAMASSPWAPLVAIAGHKQVLLYRTTDHHL
                     VGVLPFAEGLIYVLKFSRNGDLLLAGGGRGGQSGIAVAWDVKKGTRVFEVGKEYDVVL
                     AADISTDHSQVALGGPGKVVRIYNTSDGSLAQEVRKHTEWITAAEFSPDGVLLATGDR
                     NNGLIVSEVNTGREFFDLRGHQAAISDVSWRLDSNVLASASEDGSIKLWEMENGGSIK
                     SFGAHGGGVASVRFAKDARLLTTGRDHKVRLWDQNGGKQREFEPFADLALEAVFTRDD
                     TQVIAADWTGEIRAWDARDGRRLANLTVNPAPIAARIVQTKQALVATEAEADSLAKQL
                     APLQSAIAPAAAVVADAQSNAAAAEQTVVKQTALVQQLEVVQKSKHAAADDARAIAQA
                     ADRLAGQSNSALAAAENAIEQSAQGEKAAMEAVATARSAIDKSLADKVAHDHALAAAT
                     AALKGAATPEAETLAAAALTKQVQKSVQLTQAIGASGTRQSAAAQALAHARVVKQAAP
                     QAVASARTTVKATALAVQSAREACSRADQELVAADKAIVDARTLLQTVTAALTAAKQE
                     HAQASGVKAGADKALAEKKAAADAALAKVRALKAELDALAVEKKRSDAKGGDGLAAAA
                     RPAS"
BASE COUNT          385 a          762 c          727 g          460 t
ORIGIN      
        1 aaattgcgcg tccaaggatc ctcaccggtt gtcggatcga cgccagatct cggaagaatt
       61 gaaaaacccc cgagacggcc cagcctccga tcgaagccgt gccgtacaga gaatgcgcac
      121 acgcgcgcga tcgggattca cgcctccttt ccaaggcgct ccgccgcgac ttacgtcaca
      181 ggcgcaggag tgcgcatatt cgtccagatt catcggccag atggtgccgg acacagccgg
      241 attcgatcga tgtaaggccc cggatattgg cgacttgtgg ccgaccgatg tccgcgtcga
      301 ccagggcgag ttggcactcg agaccttccc aggagtgcca tttagtgcca taaagtgcca
      361 ctcgttttct gacgtaacta tgcagatgga ctatcaagcc aaacacgagt tgtgagttga
      421 acacataccc ttgtgagttc gctcgttcac gacgccggcc gcgctgcggc cgccaggccg
      481 tcgccgcctt tcgcgtcgga ccgcttcttt tcgacagcga gcgcatcgag ctcggccttc
      541 aaggcccgaa ccttggcgag ggcggcatcc gcggcagcct tcttctcggc aagcgcctta
      601 tcggcccccg ctttgactcc gctcgcctgg gcgtgctctt gcttggcggc cgtcaaagcg
      661 gctgtcacgg tctggagcaa cgtgcgtgcg tcaacaatgg ctttatcggc cgcaacgagt
      721 tcctggtccg ctcggctgca ggcttcgcgc gcggattgga cggccaatgc ggtcgccttg
      781 acggtcgtcc gcgcactcgc cacggcctgc ggagcggctt gcttgacaac cctcgcatgg
      841 gcaagcgcct gggccgcggc ggactgacgc gtgccgctgg caccaatcgc ctgtgtgagt
      901 tgcaccgatt tttgaacctg cttggtcagc gcagcggctg ccagggtctc ggcctcgggc
      961 gtggccgcgc ccttcaaggc cgcggtcgcc gcggccagcg cgtgatcgtg ggcgacctta
     1021 tctgccaggc tcttgtcgat agcggacctg gccgtggcca cggcttccat ggcggccttc
     1081 tcgccctggg ccgattgctc gatcgcattt tcggctgcag ccaaagccga gttcgattgg
     1141 ccggcaagcc gatcggcggc ctgcgcgatc gcccgtgcgt catcggcggc cgcgtgcttc
     1201 gacttttgca caacttcgag ctgctggacc aaggccgtct gcttgacaac tgtctgctcg
     1261 gccgctgcgg cattgctctg ggcatccgcc acgacagcgg cggcaggtgc gatcgcactt
     1321 tgcaaggggg cgagctgttt cgccaacgaa tccgcctcag cctcggtggc gaccagagcc
     1381 tgcttggttt gcacgatccg cgcggcgatt ggagcaggat tgacagtgag attggccaac
     1441 cggcgcccgt cgcgtgcgtc ccaggcgcgg atctcgccgg tccagtcggc cgcaatgacc
     1501 tgggtgtcgt cgcgcgtgaa gaccgcctcg agagccagat cggcaaaggg ctcaaactca
     1561 cgctgtttgc cgccattctg atcccagagg cggaccttgt ggtcgcggcc ggtcgtcaac
     1621 agccgcgcat ccttggcgaa tcgcaccgag gcgactcctc cgccgtgagc tccgaaactc
     1681 ttgatgctgc cgccgttctc catctcccag agcttgatac taccgtcttc gctggccgac
     1741 gcgaggacat tcgagtcgag ccgccagctc acgtcgctga tggccgcctg atggccacgc
     1801 agatcgaaga attctcggcc ggtgttcact tcggagacga tcagcccgtt gttgcggtcg
     1861 ccggtcgcga gcaagactcc gtccgggctg aactcggcgg cggtaatcca ttcggtatgt
     1921 ttgcgcactt cctgagccag cgatccgtca gatgtattgt aaatccggac gactttgccg
     1981 ggtccgccga gtgcgacctg gctgtggtcg gtgctgatgt cggccgccag aacgacgtca
     2041 tactccttgc cgacctcgaa gacgcgggtt ccttttttca cgtcccaggc gaccgcgatt
     2101 ccggattgtc cgccgcgtcc gccgcccgcc aagagcagat cgccgttgcg gctgaacttg
     2161 agaacgtaga tcaagccctc ggcgaaggga aggacaccga ccaagtggtg atcggttgta
     2221 cgataaagca gcacctgttt atggcccgcg attgccacga gaggcgccca tggactcgat
     2281 gccatcgcca cgaccgcgct ccgcttggcc tggggaacga acggctcggt cgtg
//
LOCUS       DATAQL010000864         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3447370, whole genome shotgun sequence.
ACCESSION   DATAQL010000864 DATAQL010000000
VERSION     DATAQL010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447370"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VHS97_14980"
     CDS             complement(<1..358)
                     /locus_tag="VHS97_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449557.1"
                     /translation="MTTELTRRDLLTGSTAVLAGGLLSTGGAFHSSGPVAGAAEPSTA
                     LGAGSQPRPDPFLLGLNTSTIRGQKLSIVEEIAIAAQAGYQAMEPWIDELERYAAPGG
                     SLEDLGKRFRDAGISVE"
     gene            complement(469..1317)
                     /locus_tag="VHS97_14985"
     CDS             complement(469..1317)
                     /locus_tag="VHS97_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3449558.1"
                     /translation="MTAPEREGSAVVAASELATEVRGQGCAVPVTHGPEKAATRAEGP
                     PAGNCRSQRPSVESREKSPSAHFRDRSITSTLWYHSVRYVLATLATIIFRWRATGQRN
                     VPVAGGVLLVSNHLSFLDVVFLGVPLRRPLNYVARSTLFVSVLGWFIRSVGAFPIQRE
                     GIGASGMKETLKRLRAGGIVTLFPEGTRSRDGKLGPLKPGIAVLVQRTGVPVVPAGLA
                     GTFEIWPRSRLIPVPHPIRIHYGPPIYPAELVGLDAEAVTALIRDRMEQSQLEASRAL
                     VCDMMY"
     gene            complement(1314..2012)
                     /gene="cmk"
                     /locus_tag="VHS97_14990"
     CDS             complement(1314..2012)
                     /gene="cmk"
                     /locus_tag="VHS97_14990"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX3449559.1"
                     /translation="MHRVVTIDGPAGAGKSTVAQRLADRLGWRFLDTGAMYRAVTLAA
                     VRGEVDLTNESALAGLVESLSVRLIGDRVMLGDEDITQLIRGIEVTQASKFVADSPSV
                     RQRLMVWQRAIAAESNVVTEGRDQGTLVFPNAFRKFYLTASDLVRARRRVAEYEARGE
                     AASFESVLSAQRDRDARDAARVIAPLKPALDAVVIDTTHLSIEAVVDRMVFEIEERLT
                     GGRVGVESSLGVRS"
     gene            complement(2012..>2315)
                     /locus_tag="VHS97_14995"
     CDS             complement(2012..>2315)
                     /locus_tag="VHS97_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX3449560.1"
                     /translation="DRRKASTDPYSEELAQSQAELDAEEQKLTSYIEELKRLGVEFKG
                     PDGLCDFYSMKDGREVLLCWRLGEPVVAHWHDLDAGFAGRRPLTDHAGESNEEDLF"
BASE COUNT          475 a          761 c          664 g          415 t
ORIGIN      
        1 tctcgacact gatgcccgcg tcgcggaaac gcttgcccag atcttcgagc gagccaccgg
       61 gcgcggcata gcgctccagc tcgtcaatcc agggttccat cgcctgatag ccggcctggg
      121 cggcgatcgc aatttcttca acgatcgaca gcttttgtcc acggatcgta ctcgtgttca
      181 agcccagcaa gaacggatcc ggcctcggtt gggacccggc accaagggcc gtggagggct
      241 cggccgcgcc ggctaccgga ccagacgagt ggaaagctcc gcccgtactt aggagccctc
      301 cggccagcac tgcagtgctc cccgtcaaca gatcgcgtcg tgtcagctcg gtcgtcacgt
      361 cgggcctcat ctcaaggtgg cacaggcatc ttgcccgtaa tcatccactg cctggacggc
      421 cgtgccacgt gggcaggcgc caaggtggat ggggtcattg accaatcgtc agtacatcat
      481 atcgcaaacc aaggctcgag aagcttccaa ctggctttgt tccatgcgat cgcgtatgag
      541 cgctgtgacc gcttcggcgt cgaggcccac aagttccgcc ggataaatcg gcgggccgta
      601 atgaatccga atcggatgcg gaaccgggat cagccgtgac ctcggccaga tctcgaacgt
      661 gccggccagc ccggccggga cgacaggaac accggtccgt tgaaccagga cggcaatccc
      721 cggcttgagg gggccaagtt tgccgtcgcg gctacgcgtt ccctccggaa aaagagttac
      781 aatgccgccg gcacgcagac gctttagcgt ctctttcatg cccgacgccc ctatgccttc
      841 gcgctggatg ggaaaggccc cgaccgaacg aatgaaccag cccaggaccg acacaaacaa
      901 ggttgatcgc gcgacgtagt tgagtggccg ccgcaagggc acgccgagaa aaaccacatc
      961 gaggaaactg agatggttag agaccagtaa gactccgccg gcaaccggaa cattacgttg
     1021 accagtcgcc cgccagcgaa aaatgatcgt cgccaaagtg gcaaggacat atcggaccga
     1081 gtggtaccac aatgtcgaag tgatcgagcg atcgcgaaag tgtgcgctcg gcgatttctc
     1141 ccgcgattcg accgacggac gttgcgatcg acaattcccc gccggcgggc cttcggcacg
     1201 cgtggcagct ttctctggac cgtgcgtaac cgggaccgcg cacccctgac cgcgtacctc
     1261 agtcgcaagt tcgctggctg cgacgacggc gcttccctcg cgttctggtg ccgtcatgag
     1321 cgaactccta acgacgattc gactccgaca cggccgccag tcaatcgttc ctcgatctcg
     1381 aaaaccatgc ggtcgactac agcttcgatg gaaagatgcg tggtgtcgat tacgacggca
     1441 tccaaggcgg gtttcaaggg tgcgatcaca cgagcggcat cgcgcgcgtc gcgatcgcgc
     1501 tgagcgctca gcacggactc aaagctcgcc gcttcacctc tcgcctcata ctcggcgacc
     1561 ctgcgccggg cccgcactag atcgctggcc gtcaagtaaa acttgcgaaa ggcattagga
     1621 aaaaccagcg tcccctggtc acggccttcg gtcacgacat tcgattcggc ggcaatcgca
     1681 cgctgccata ccatgagccg ctggcgaacg ctcgggctat cggcgacgaa tttcgatgcc
     1741 tgggtcactt caatcccgcg gatcaattgc gtgatatctt catcgcccag catcacgcgg
     1801 tcgccaatca agcgaacgct cagcgattcg acaagcccgg caagcgcgga ctcgtttgtc
     1861 aaatcgacct caccacgtac cgccgccaac gtgaccgctc gatacatcgc accggtgtcg
     1921 aggaagcgcc agcccagccg atctgccagc cgttgcgcga ccgtgctctt acccgcccct
     1981 gcgggaccat cgatggtgac gactcggtgc atcaaaaaag atcttcttca ttggactcac
     2041 cggcgtggtc ggtcagtggc cggcgaccgg cgaagccggc gtccaaatcg tgccagtggg
     2101 ccaccaccgg ttcaccaagc cgccagcaga gcaatacctc acggccgtcc ttcatgctgt
     2161 agaaatcgca cagaccgtcc gggcccttga actcaacgcc cagccgcttc aattcttcga
     2221 tgtacgacgt cagtttttgt tcctcggcat cgagctcggc ctggctctgc gccaattcct
     2281 ccgagtacgg atcggtcgag gccttgcggc gatcc
//
LOCUS       DATAQL010000865         4823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1464219, whole genome shotgun sequence.
ACCESSION   DATAQL010000865 DATAQL010000000
VERSION     DATAQL010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464219"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1931
                     /locus_tag="VHS97_15000"
     CDS             216..1931
                     /locus_tag="VHS97_15000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX3449561.1"
                     /translation="MKGYIPQTFPHQWLPEKLELKTWDQIEPWYQKLMEQPINTPLDL
                     ERWVVAAGELNAAVGQEGVERYIAMTCQTDDPEREAAYLQFVRDIEPKLKPIQNAIRE
                     RYLDSPYRSLLPQSRYFVFDRALANRRDLFREANIPRETKLAELEQQYQKTLGAMTVT
                     YRGQERTLAQMAPFLEETDRAVRAQAWELAANRRLVDREALDLLFDQMKQLRIEVAAE
                     AGFGNFIEYAFRNRERFDYGIADTLRFHEAVERVVVPLMRKIQEKHRSTLKVESLRPW
                     DTAVDPLGRPPLVPFKDVEKLAAGTESIFRDVDMELGEQFSFLRSEQLLDLANRKGKA
                     PGGYQTTLEDRRLPFIFMNAVGLDGDVRTLLHEGGHAFHTLACRAEPLSAYRESPLEF
                     CEVASMTMELLGARRLDPFYESAEAGRSNRKLLEGIVLILPWIATVDAFQHWIYSHPD
                     HTRLQRKTAWRELLDRFGGIVDWSGHEEARDHLWHRQLHIFLYPFYYIEYGIAQLGAL
                     QIWQRSLRDRQGAVADYKKALALGGSRPLPELFAAAGARFDFGDRLISSLMETIEGEL
                     EKLSP"
     gene            complement(1939..4224)
                     /locus_tag="VHS97_15005"
     CDS             complement(1939..4224)
                     /locus_tag="VHS97_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449562.1"
                     /translation="MRAHAALVLALALVGSVLGGLLVGYEPVGGDPDRLYRPLKSELA
                     RALKEGRLPFWSSRFGLGVPLVAESHVAAFYPLNIVLYGLIDLSVAYRLSMWLHYVAL
                     VGTTYLYARCLGLSKWGSALAGVAFALCGFQAIHSSHEPFYSVMPYLPLALWITERYL
                     GNGRIFSLALLALCLGVQWSLGHFQLQTWTGGLVVLTGLWRAFFDRRAWGRALGVIAG
                     TAWGAMLAAVQLGLSWQLAGAVRQTGRSASDLTYYSYPPSHWFELVLPRLVRELRLGP
                     EDPYWFGQQTWGYEAALYVGTIPVILAFIGLCGRPPSRSAGLWRLLVPASLAIATMPR
                     WWPQGYLHLISLPGIGYFRAPARYTALTSLGLAVLAGEGFDRAISTTRFRAGLSASLV
                     FAGCAAVAAVLWTNRRDVRLVSVLGGTIDGFVWAGLAWLVALIVVLAWRLQRLPSWAP
                     VIALGVELGILFYASTTQWDWAIAVPAQSPVLTELMGRSPPGSVGGETENLPVRVGLA
                     TSYPYLGFAHADANRSLVVAQLRLVRGDAASSLTSLHAQALKRWLRRCRVQYLAGSHR
                     SILGLGNPLGRWRDSALDQMIYHGPTEAANRVWSIVQLEEPFPEARVARRARTIANQE
                     NLIERLFQSDDLDLAWFLAEDRVPARAEARSARLVSWDGMAAMVEHAGDCDLVIARTF
                     DAGWQARIDGEPEQPVLSADGGFLAVRVPGSGTHRIRLRYQPQRLALYSAISLVSAAS
                     IAGVIATALWNASRRKTYATA"
     gene            4388..>4823
                     /locus_tag="VHS97_15010"
     CDS             4388..>4823
                     /locus_tag="VHS97_15010"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3449563.1"
                     /translation="MMAIAMADRFRKWYEHEKDAHANVVRSLESVPPDQRTSPEFQKA
                     VCWLAHLAMARRVWLERLGVIPAKTSGPFFPEGKELADTIADLKEVQELWSHYLAKLS
                     DDELAQRIEYNSLDAGRFGNRIEDILTQLFTHSAYHRGQIASL"
BASE COUNT         1041 a         1517 c         1419 g          846 t
ORIGIN      
        1 gcccacgcgg aagctgtggc cacgacggcc ctcgtcttaa tcatcgccac cacaatcatg
       61 tcatcgtggc tcttcgctgg ggcatggctc gtccatgctt cgataccttg actatggtta
      121 tgatcaaacc gggtctgagc cgccaaatca cgatatcgat gacggaactc acataaccga
      181 ctcctctgcc ctaaagggag tccaggagaa cgaaattgaa gggttacatt cctcagacat
      241 ttccccacca atggcttccc gagaaactcg agctcaagac ttgggatcaa atcgaaccgt
      301 ggtatcaaaa actgatggag cagccgatca atacgcccct tgatctcgaa cggtgggtgg
      361 tcgcggcggg cgaattgaac gcggctgtcg ggcaggaagg agtcgagcgc tatatcgcga
      421 tgacctgtca gaccgacgac cctgagcgcg aggccgccta tctgcagttc gtacgggaca
      481 tcgaacccaa gctgaagcca attcaaaacg cgatccgcga gcgctacctc gactcgccgt
      541 atcgatccct tctgccgcaa agtcgctatt tcgtttttga tcgagcgctc gccaatcgcc
      601 gcgacctgtt tcgcgaggcg aacatccccc gcgagacgaa gctcgctgag ctcgagcaac
      661 agtatcaaaa gaccttgggc gccatgaccg tgacctatcg cggtcaggaa cgcaccctgg
      721 cgcagatggc tccgtttctg gaagagacag accgcgcggt ccgcgcccaa gcatgggagc
      781 tggctgccaa ccggcgtctc gttgatcgcg aggcactcga tctcttgttt gaccagatga
      841 aacagctccg tatcgaggtc gccgccgaag cgggattcgg caatttcatc gagtacgcgt
      901 tccgcaatcg cgaacggttc gactacggca tcgccgacac gcttcggttt catgaagccg
      961 tcgagcgcgt cgtggtcccc ttgatgcgca aaatccaaga aaagcacaga agcacgctca
     1021 aagtggaatc gctccgaccg tgggatacgg cggtcgaccc cctgggcagg ccgcccttgg
     1081 taccattcaa ggacgtcgaa aaactggccg cgggaaccga gtctatcttt cgcgacgtgg
     1141 acatggagct tggtgaacag ttttcgttcc ttcgatccga gcagttgctc gacctggcca
     1201 accgcaaggg gaaagccccg ggcggttacc agacgaccct tgaagacagg cgtttgccgt
     1261 tcatcttcat gaatgcggtc ggactcgacg gggacgtgcg aacattgctc cacgaaggtg
     1321 gacatgcctt tcataccctg gcctgccgcg ccgagccgct gtcggcctat cgcgaaagcc
     1381 ccctcgagtt ttgcgaggtc gcctcgatga caatggaact tttgggcgcg cgccggctcg
     1441 acccgttcta cgaatccgcc gaggccggcc gctccaaccg caagctgctc gaaggtattg
     1501 tactgatcct cccctggatc gcgacggtcg acgcgtttca gcactggatt tactcccacc
     1561 ccgaccatac ccgcctccag cgaaaaaccg cgtggcgaga attgctcgac cgcttcggcg
     1621 gaattgtcga ttggtccggc cacgaagagg cccgcgacca tttgtggcat cgccagcttc
     1681 acattttctt gtaccctttt tactacatcg aatacggcat cgcgcagctc ggggctctcc
     1741 agatctggca gcgatcgttg cgcgatcggc agggggccgt cgccgattac aagaaggcgc
     1801 tggcgctggg aggctcgcgg ccgctccccg aacttttcgc cgcggccggt gcgcggtttg
     1861 atttcggcga tcggctcatc tcctcgctga tggagaccat cgaaggggag ctcgaaaagc
     1921 tgagtccgta aagcaagctt aggctgttgc atacgtcttc cgacgagacg cattccaaag
     1981 ggcggtcgcg atgacgccgg cgattgacgc tgccgacacc aatgagatcg ccgagtacag
     2041 cgcgagccgc tgcggttgat aacgtaggcg aatccgatgc gtgccagagc ctggcacgcg
     2101 cacggccagg aagccgccgt cggccgacaa caccggttgc tcgggctcac catcgattct
     2161 cgcctgccag ccggcgtcga acgtgcgcgc gatcaccagg tcgcagtcac ctgcatgctc
     2221 gaccatcgcc gccattccat cccacgacac gagccgggcg gatcgtgcct ccgctcgagc
     2281 gggcacacgg tcttcggcta gaaaccaggc gagatcgaga tcgtcggatt ggaagagccg
     2341 ctcgatcaga ttttcttgat tggcgatcgt gcgggctcgt cgagccacgc gcgcttcggg
     2401 gaatggctct tcgagctgga cgatcgacca gacccggttt gccgcctcgg tcggaccatg
     2461 ataaatcatc tggtcgagcg cggagtcgcg ccagcgtccg agcggattgc ctaaccccag
     2521 tatggatcgg tgagaaccgg caagatactg cacgcgacag cgcctcagcc agcgcttgag
     2581 cgcctgggcg tgcaggctag tgagagagct agcggcatcg cctcgtacca gacgcaattg
     2641 agcgaccacg agactccgat tggcatccgc gtgggcgaaa cccaggtagg gataggaggt
     2701 cgccaggccg acgcggaccg gcaggttttc tgtctcgccg ccgaccgagc ctgggggcga
     2761 ccggcccatc agttccgtca agaccgggct ttgcgcgggg actgcgatcg cccagtccca
     2821 ttgggtggtc gaagcgtaga acaaaattcc cagctcgaca ccaagcgcga tcacgggcgc
     2881 ccacgacggg agcctttgga gtcgccaggc caagacaacg atcaatgcaa cgagccaggc
     2941 cagaccggcc cacacgaatc cgtcgatcgt tccacccagg acggacacaa ggcggacatc
     3001 ccgtcggttc gtccagagaa ctgcagcaac cgcggcgcaa ccggcgaaga cgagagatgc
     3061 cgagaggccc gcgcgaaacc tcgttgttga aatcgcccta tcgaatccct ccccggctag
     3121 caccgccaag cccagactcg tcagtgcggt gtatctggcg ggcgcgcgga aatagccgat
     3181 ccccggcagt gaaatcagat ggagatatcc ttgcggccac cagcgtggca ttgtggcgat
     3241 cgccaagctg gctggcacta gcaacctcca caagccggct gagcggcttg gtggacggcc
     3301 acataaaccg atgaaggcga ggattaccgg gatcgttccg acgtacagag cggcctcgta
     3361 tccccaggtc tgctggccaa accagtatgg gtcttccggc ccgagccgca actcccgtac
     3421 gagtcgcggc agcaccagct caaaccagtg cgacggagga tacgaatagt aagtcaaatc
     3481 gctggccgag cggcccgtct ggcggacggc gccagccaat tgccagctca ggccgagttg
     3541 aaccgccgca agcatcgctc cccaggcggt cccggcgatc acgcccagcg cccgacccca
     3601 ggcgcggcga tcgaaaaacg ctcgccacag cccggtcaag accaccagtc ctcctgtcca
     3661 agtctgaagc tgaaaatgtc ccagggacca ctgcactccc agacacaaag ccaaaagtgc
     3721 cagcgagaaa atccgtccgt tccccaggta gcgctcggtg atccagagcg caaggggcag
     3781 gtagggcatc acactgtaaa acggttcatg gctcgagtgg attgcctgaa agccgcagag
     3841 cgcaaacgcg acaccagcga gcgcgcttcc ccacttggaa aggcccagac agcgcgcgta
     3901 gagataagtc gtgcccacga gcgccacgta atgaagccac attgacagcc gataggccac
     3961 cgagaggtcg atcaaaccgt agagcacgat gttgagaggg taaaaagccg cgacatggct
     4021 ttcggcgaca agcggcacac ccagtccgaa acgactgctc cagaacggca gccggccctc
     4081 cttgagcgcg cgggcgagtt cgctcttgag cggccgataa agccggtccg ggtcgccccc
     4141 gaccggctcg taaccgacaa gcagcccacc caggacactt cccacgagcg caagcgccag
     4201 cacaagtgca gcgtgagcgc gcattgcccg cgaccacctc tcgggccagt ttggacgatc
     4261 tgctatgccg gcattaacca ttgaacgtga tccgaatcgt aagtcgcgtg cagcttgccc
     4321 gcgatgtcat tgaaccacca cttggtataa tcagtgacgt gcactaatgg tttcacccgg
     4381 agcccgcatg atggcaatcg caatggccga tcgattccgt aagtggtacg agcacgagaa
     4441 agatgctcac gccaacgttg tgcggtcgct ggaatccgtt ccgcccgacc agcggacttc
     4501 gccggaattc caaaaggcgg tatgttggtt ggctcatctg gcgatggcac ggcgagtgtg
     4561 gctcgaacgg ctgggtgtga tcccggccaa gacgtctggt ccgttcttcc cagaggggaa
     4621 ggaacttgcc gatacgattg ccgatctcaa ggaagtccaa gagctctggt cccactatct
     4681 cgctaaactg tctgacgacg agctggccca aaggatcgaa tacaatagcc tcgatgctgg
     4741 tcgatttggc aaccgcattg aagacattct cacgcagctc ttcacacact cggcgtacca
     4801 ccgcgggcag atcgcgtcgc ttg
//
LOCUS       DATAQL010000866         5029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2382326, whole genome shotgun sequence.
ACCESSION   DATAQL010000866 DATAQL010000000
VERSION     DATAQL010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382326"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(755..2584)
                     /gene="mnmG"
                     /locus_tag="VHS97_15015"
     CDS             complement(755..2584)
                     /gene="mnmG"
                     /locus_tag="VHS97_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX3449564.1"
                     /translation="MRDSYWFDVVVVGAGHAGLEAALAAARMGARTALLTMNCDTVGQ
                     MSCNPAIGGVAKGQIVREIDALGGAMGIFTDATAIQFRLLNRGKGPAMHSPRAQCDKK
                     AYQFMAKLAVERQANLTIRQEMAEAILVEGDRAVGIECRGKVQYKARAIVVTTGTFLQ
                     ALMHTGEVKTAGGRGGDAPAVGLSQSLLSLGFVLERFKTGTPPRLNGRTIDFSRLEPQ
                     PGDADPVAFSFLTDAITEPQIDCHITYTNPEVHNLIRANLHRAPMYSGQIQSTGPRYC
                     PSIEDKVVRFADRDSHQIFLEPEGRNTLEYYCNGISTSLPRDVQEAIIPLIPGLEQAE
                     IMRYGYAVEYDFAPPTQLFTTLETKQVRNLYFAGQINGTTGYEEAAGQGLLAGINAAL
                     QIKDEAPVILDRSRAYIGVLIDDLVTRGVDEPYRMFTSRAEYRLMLRHDNADLRLTEL
                     GRRVGLVDDHRWERFTRRRDAIGHLRDRLTRTRVAGDSLFQALRRPETTWFDLRSLDP
                     ALVEAGFAADVIDQVTIEAKYDGYIGRQLDQVERFRRMEEKRIPAELDYRSIPQLRAE
                     AREKFERVRPASVGQAGRISGVSPADIATLVIHLKRREPETSS"
     gene            2816..3346
                     /gene="tpx"
                     /locus_tag="VHS97_15020"
     CDS             2816..3346
                     /gene="tpx"
                     /locus_tag="VHS97_15020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX3449565.1"
                     /translation="MAEARKGEVTFKGNPITLVGPKLKPGDKAPDFKAVGAGLALVSL
                     ADTAGKARLFNVVPSLDTPVCNKQTKRFAEELKALGDNVAAFTVSTDLPFAQARFCTE
                     ASIENIKNLSDAHNHSFGEHYGVLISDLPVPLLARAVFVVDPSNTIRYAEIVPEIGQE
                     PNYEPALTALKAAAGA"
     gene            complement(3356..4048)
                     /locus_tag="VHS97_15025"
     CDS             complement(3356..4048)
                     /locus_tag="VHS97_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3449566.1"
                     /translation="MRFISPLFLLISVALCPLYLQAVQSDVKSHDDLLGAKPPEGAVV
                     VFGGSDLKGWVKTDGKTPADWPVEGHVFTVAKGNIMTSQRFGNFQLHLEFNVPYMPSK
                     RGQGRGNSGVYLTGNHELQILDSYKLTLHNDDCGAIYKQIIPSVNACKPPLQWQSYDV
                     TFHKAVVEGGKVVKKARVTVIHNGIKTIDDAEISPTPGGIGVAEGQDGPLMLQDHGNP
                     VEFRNIWIKPLD"
     gene            4184..4948
                     /gene="grpE"
                     /locus_tag="VHS97_15030"
     CDS             4184..4948
                     /gene="grpE"
                     /locus_tag="VHS97_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX3449567.1"
                     /translation="MPEINDTADAVHGPEDSDDLLVPEGAVSLPGDDFDLEIIERLAE
                     EQQHVQTAVVSGAGGGTAGEPALDPRQETAAAVDATSVLGSVERLAEQLGRRLDLLQT
                     TFERELRAESTRERVVDRLHAELQEYKQDFLLKVQRPIFIDLIQLHDDIGKMIDTGTS
                     TGADPDAGVRLRGTLESIQTAIEDILYRQGVEPFTLDGTEFDPRKQRAVSTHATDDPA
                     LNKRVATRLRKGFCSGDKLIRPEIVTVFTHRPLAPA"
BASE COUNT         1016 a         1491 c         1484 g         1038 t
ORIGIN      
        1 cgagtcaaag cagcgaatga tcgtctgctg actcacttta cagaccttgg ccgcctcgcc
       61 tgtcgtgaaa accgtcttca tcgttgtgta ggtcccatct tgcgagatca aggacgtggt
      121 cctccctctc ccagttaatg ggaacatgat cggaggagta agacgtcgcc tgatcggcct
      181 ccgtgcaaac ccatctcacg acccgaaccc aaccgtcgat tcactcctgc ccaactccgt
      241 cgatcgaacc aaacctatca tcttcgcgcc gtgttaaaat ctacgaaacc ttcagatatt
      301 accaatatta ccaatctctc caattgtaga aaagccccta accctgtcaa tccgaatagc
      361 gttggcgctc gaagtttcac tacttcgccc ttgttccgaa tatagattgc tgcgggagtt
      421 ttaacgactt aatcaatttt atgccaggaa gaatagagaa aaaaagtgaa ttgcgtatca
      481 gctgccggct ccaagatggt aaacttcggc caagttgaca agagaaacta atgagggtat
      541 tggcgactgt gccactcttt ggggatgtgt gacgaactga catcgtgtcg gtgaactgag
      601 aagatccgat gaactgggaa tgaactattc tggcaagctt tagtgaacga tcaatgccca
      661 tcaacgaaat cgttcggacc gctcatccaa ctgtcaaatt gtcgggtgaa cgacaatttc
      721 agcgagggcc cgatgactgc caagaacggg gactctacga agaggtctcc ggctctcgac
      781 gtttgaggtg aatcacgagt gtggcaatat ccgccgggct gacgccgctg atccggcccg
      841 cctggccaac tgatgccggt ctgacgcgct caaatttctc ccgggcctcg gcgcgcaatt
      901 gcggaatcga acggtagtcg agttccgcgg ggattcgctt ttcttccatc cgccggaagc
      961 gctcgacttg atcaagctgc cggccgatat agccgtcgta tttggcctcg atcgtgacct
     1021 gatcgatcac gtcagccgca aagcccgcct cgaccagcgc cgggtcaaga gaacgcaggt
     1081 cgaaccaggt tgtctcgggt cgacgcagtg cctggaataa ggaatcgccc gcgacgcgtg
     1141 tgcgcgtgag gcggtcgcgc aaatggccga tcgcgtcacg ccgacgcgtg aagcgctccc
     1201 agcggtggtc gtcgaccaga ccgacgcgcc tacccagctc ggtcagcctc aggtcggcgt
     1261 tgtcgtgacg gagcatcagg cgatactcag cgcggctggt gaacatgcga tagggctcgt
     1321 cgacgccgcg cgtgaccagg tcgtcgatca gaacgccgat gtatgcgcgc gaccgatcga
     1381 ggattacagg tgcctcgtct ttgatctgga gcgccgcgtt aataccagcc aagagcccct
     1441 gtccagcagc ctcctcgtaa cctgtcgtgc cgttgatctg accggcgaag tacagattgc
     1501 gcacttgctt ggtctcgagg gtggtaaaaa gttgggtcgg tggtgcgaaa tcgtattcga
     1561 ccgcatagcc gtaccgcatg atctcggcct gttcgagccc cggtatcaga ggaatgatcg
     1621 cctcctggac gtcgcgcggg aggctggtcg agatgccgtt gcagtaatac tcgagagtgt
     1681 tacgcccttc tggctcgagg aaaatctgat ggctgtcgcg gtcagcaaac cggacgacct
     1741 tgtcttcgat cgacggacag tagcgcgggc ccgtgctttg gatttgcccg gaatacatgg
     1801 gtgcgcggtg tagattggcg cggatcaggt tgtgaacctc cggattggtg taggtgatat
     1861 ggcagtcgat ctggggttct gtgatcgcat ctgtaaggaa cgaaaatgca accgggtcgg
     1921 catcgcccgg ctgtggctcg agccgcgaga agtcgatcgt gcgcccgttt aatcgcggcg
     1981 gcgttccggt cttgaatcgc tcgagcacaa atccgagtga aaggaggctc tgcgacagcc
     2041 caaccgcggg cgcgtcaccc ccccggccgc cggcggtctt gacctcgcca gtgtgcatga
     2101 gcgcctgaag aaacgttccg gtggtcacaa cgatcgcgcg ggccttgtac tggaccttac
     2161 ctcgacactc gatgccaacg gcgcgatctc cttcgaccag aatcgcctcg gccatctcct
     2221 ggcgaatcgt caggttcgct tgccgttcga cggccagctt ggccatgaac tgataggctt
     2281 ttttgtcgca ctgggcgcgc gggctgtgca tcgcgggccc tttgccccga ttgagcaacc
     2341 ggaattgaat tgcggtggcg tcggtaaaga tccccatcgc gccgcccaag gcgtcaatct
     2401 cacggacaat ctggcccttg gcgactccgc cgatcgcggg attgcagctc atctggccga
     2461 cagtatcgca gttcatcgtc aaaagcgctg tgcgtgcgcc catgcgggcg gctgccagcg
     2521 cggcctccag gccagcgtgg ccggccccga cgacgaccac gtcgaaccag taagagtcgc
     2581 gcatgtttct agtcccttca atgacctggc cgtgtccgaa aggccaggac acctcgcgtt
     2641 ctgcggcagc actcatctta tacggcggcg cgcgggcgaa gaaagacgag ccgatccgcg
     2701 acgacgggcc ttgtccgcca ccggcttgca tcgcgttgtc gccagccgct accctgaatg
     2761 agctagtgcg gagtctcgct cttttgtcag ttccgatctt cgaggacaat cagctatggc
     2821 cgaagcgcgc aaaggcgaag tgacgttcaa ggggaacccg atcacattgg tcgggcccaa
     2881 gctcaaaccc ggtgacaagg ctccggattt caaggcggta ggggcggggc tggccttggt
     2941 gtctctcgct gacacggcag gcaaggctcg tcttttcaac gtggtcccct cgctcgacac
     3001 acccgtctgc aacaagcaga ccaagcgttt cgcggaagag ctcaaggctc tgggcgataa
     3061 cgtggcggcg ttcactgtaa gtaccgattt gccgtttgcc caggcccgtt tctgcacgga
     3121 ggcgagcatc gaaaacatca agaatctctc cgatgcccac aaccacagct tcggcgaaca
     3181 ttacggcgta ttgatcagcg acctgccggt tcccctcttg gcgcgagccg tctttgtcgt
     3241 cgatccttcc aatacgattc gctatgccga gatcgtaccc gagatcggcc aggagcccaa
     3301 ctacgagccg gctctgacgg cactcaaagc agcggccggc gcgtgatcca cgctctcaat
     3361 cgagcggttt gatccagata ttgcgaaact cgaccgggtt gccatggtcc tggagcatga
     3421 gcggcccgtc ctggccttcg gcgacgccga ttccacccgg cgtgggcgag atctcggcat
     3481 cgtcgattgt cttgatcccg ttgtgaatca cggtcactct tgctttcttg acgaccttgc
     3541 ccccttcaac cacggccttg tgaaacgtca cgtcgtatga ctgccattgg agcggtggtt
     3601 tgcaggcgtt gaccgacggg atgatctgtt tgtagatcgc gccgcagtcg tcgttgtgca
     3661 atgtcagctt gtaagaatcg agtatttgga gctcgtggtt ccccgtcaga tagacgccgc
     3721 tgttaccgcg cccctgcccg cgcttgctgg gcatataggg cacgttgaat tcgagatgga
     3781 gctgaaagtt gccaaagcgc tggctggtca tgatattgcc cttagcgacc gtgaagacat
     3841 gcccctccac gggccagtcg gcgggagtct tcccatccgt tttcacccaa cctttcaggt
     3901 ccgagccacc aaagacgacg acggcgcctt cgggcggctt ggcgccgagc aagtcgtcgt
     3961 gagacttgac atcagactgg acggcctgga gatagagcgg gcaaagggcc accgagatca
     4021 gtaagaataa aggcgaaatg aatctcatca gatatctccc ggcagggttc ggtctggcgg
     4081 cgcgacacgg agggtacttt tttgggtcgt cgacagcagt ctaacatctc ggcgtctcag
     4141 gcgaatacat gcgggtcgtc acgcgcgcag agaggagttg accatgcccg agatcaacga
     4201 cacggctgac gcggttcacg gcccggaaga ctccgacgac ctacttgttc ccgaaggcgc
     4261 cgtctcgctg ccgggcgatg atttcgacct ggagatcatc gaacggctgg cggaagagca
     4321 acaacatgtc cagaccgccg tagtctctgg cgccggcggc ggaacggccg gtgaacccgc
     4381 cctcgatccg cgccaagaga ccgctgccgc cgtggacgca accagcgtgc ttggctctgt
     4441 tgagcggctg gccgaacagt tggggcggcg gctagacctg ctgcagacga cttttgagcg
     4501 cgagcttcgt gccgagtcca cgcgcgagcg cgtggttgat cggctccatg cggaacttca
     4561 ggagtataag caggactttc tcttaaaagt tcagcgtccc atcttcatcg atttgattca
     4621 acttcacgat gatatcggca agatgatcga taccgggact tccacgggtg cggaccctga
     4681 tgcgggtgta cgcctccgtg gaaccctgga atcgatccag acggcgatcg aagacatact
     4741 ctatcgtcag ggcgtcgagc cgttcaccct ggatggcacg gagttcgatc cccgcaagca
     4801 gcgcgcggtc tccacccacg cgacagatga tccagcgctc aacaagcggg tagctacccg
     4861 gctgcgcaaa ggattttgct caggcgacaa gttaattcgc cccgagatcg tcacggtctt
     4921 cacccaccgt ccgctggcgc cggcgtgatg tcggagcgag ccacgcccta ttacccgcgg
     4981 acgaccgccc agtcgtgggc tcgctcgatg gtcgtggcgt gaaagtttt
//
LOCUS       DATAQL010000867         3254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4822965, whole genome shotgun sequence.
ACCESSION   DATAQL010000867 DATAQL010000000
VERSION     DATAQL010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4822965"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VHS97_15035"
     CDS             <1..465
                     /locus_tag="VHS97_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3449568.1"
                     /translation="AEWKHLRPGGNSGIFVWAPEKALEGIKPNSLPRGGIEVQILDHG
                     YREQYEKKSGKKATWFTTDGDVFAVGTSKMTPFPPVSPDGHRSFPSKRLSKGVGEWNH
                     YYVRGINGEVRLWVNGEEVSGGTGCEPRTGFLCLESEGSPVEFRRLRLRELP"
     gene            481..1554
                     /gene="glpX"
                     /locus_tag="VHS97_15040"
     CDS             481..1554
                     /gene="glpX"
                     /locus_tag="VHS97_15040"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX3449569.1"
                     /translation="MSQPPDEITRPTQANSASSSAAGPEPPTRRLIATLDSNDVALDL
                     VRTTEAAALAASRWVGRGDKNASDQAAVDAMRLFFNAVHMDGIVVIGEGEKDRAPMLY
                     NGERLGTGSPPVVDVAVDPIDGTRLVARGEANALSVVAVAERGAMFNPGPCMYMEKIA
                     TGPEAGTSIDIAAPIETNIKIVALAKRLPISEVTVMILDRPRHETVVERVRKTGARVH
                     LLSDGDVAGAITAARRGTGVDLLYGIGGTPEGVIAAAALKCLGGAIQGRLYPRNDEER
                     AAAIDAGYDLERVLTTDDLVKGENVFFAATGITDGALLRGVRFSPDGATTESIVMRAH
                     SGTVRMIHGEHRVGKLKQIYGEE"
     gene            complement(1602..3008)
                     /locus_tag="VHS97_15045"
     CDS             complement(1602..3008)
                     /locus_tag="VHS97_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449570.1"
                     /translation="MASPATYDHSPQSSWWLIESARRCGTQLTVLRQEQTYPNGRQKI
                     ALVAEYLREHPEYRYVLMVDFSDVIFCATLREMFYKYRSFPKSIVISAQRNNWPLPIL
                     GGHSPETGTSSRYLNSGSIFATAEAWLSAWDTMQARERHWEGKPPEIAHGRHIFNDDQ
                     AAWIDLYVNRLSDIVLDGECRLFQVLDQVDSTVGSGNRDLLLEGRRILNRETGARPCL
                     VHGAGNVPLGAWANYILDSPPVWNWPLIQRIRQAPLKVLRNPSGVERLLLGLGLHERT
                     AAALPDELLQYSGKGLSIWQWPNQFSPYLVWLARQPPIRSYVEIGVNEGGSFITTVEY
                     LRRFHPLSLAIAVGPYLSPRVHDYVRRTAGTHFVAGNRSSQELRELVAAERGIGLTFI
                     DGEHFGTSVRADWEFARSCTRYVAFHDIATNMFPDVTSLWAEIRATYKKTYEFVAQYR
                     CLDSCAGIGVVDSAGGIA"
BASE COUNT          688 a          990 c          959 g          617 t
ORIGIN      
        1 gccgagtgga aacacctacg cccgggcgga aactcgggga tctttgtgtg ggccccggag
       61 aaagccctcg agggcatcaa gcccaactcg ctcccccgcg gcggcatcga agtgcagatc
      121 ctcgaccacg gttaccggga acaatacgag aaaaagtccg gcaaaaaggc cacttggttc
      181 acgacagacg gcgacgtatt cgcggtggga acctcgaaga tgaccccgtt tccgccggtc
      241 tcaccagacg gccatcgcag ctttccatcg aaacggctca gcaagggggt cggcgagtgg
      301 aaccactatt acgtacgcgg catcaacggc gaggttcggc tctgggtcaa cggcgaggaa
      361 gtctcgggcg gcactggttg cgaaccacga acaggctttc tctgcctgga gtcggaaggt
      421 tcacccgtcg aattccggcg gctccgactg cgcgaattgc cttaattcta gaataatagc
      481 atgtcacaac cgcccgatga aatcacccgt ccgactcagg cgaattctgc gtcatcgtcg
      541 gccgctggtc ccgagccgcc cacgcgacgg ctgatcgcca ctctggattc caacgacgta
      601 gccctcgacc tggttcgcac caccgaggcc gccgccttag cggcctcgcg ctgggtcggt
      661 agaggtgata aaaacgcctc agaccaggct gccgtcgatg cgatgcgctt attcttcaac
      721 gcggtgcaca tggacggcat cgttgtcatc ggcgaaggcg aaaaggaccg ggcccccatg
      781 ctctacaatg gagagcgcct cggcacgggc tccccgccgg ttgtcgacgt cgccgtcgat
      841 cccatcgatg gaacccgcct ggtggcgcgc ggggaggcca atgcgctgtc cgtcgttgcc
      901 gtcgccgagc gcggcgcgat gtttaacccc ggtccctgca tgtatatgga aaagatcgcc
      961 acggggcccg aggccggcac ttccatagac atcgcggcac cgattgagac gaacatcaaa
     1021 atcgtcgccc tggccaagag actgcctatc agcgaggtca cggtgatgat cctggaccgt
     1081 ccccgccacg agacggtcgt cgagcgtgtt aggaaaacgg gtgcccgtgt ccaccttctg
     1141 tccgatggag acgtcgccgg tgccatcacg gccgcccggc gcggcaccgg cgtcgatttg
     1201 ctctacggta tcggcggaac acctgaaggc gtgatcgccg cggccgccct caaatgcctg
     1261 gggggcgcca tccagggacg actgtaccca cgtaacgacg aggaacgcgc cgccgcaatc
     1321 gacgctggct acgacctgga gcgtgtcttg acgactgacg atctcgtcaa gggcgaaaac
     1381 gtcttcttcg ccgcgaccgg tatcaccgac ggagccctgc tgcgcggcgt tcgtttctcc
     1441 cccgacggcg cgaccaccga gtcgattgtc atgcgcgcgc attccggcac cgtccgcatg
     1501 atccacggcg aacaccgcgt cggcaagctc aagcagattt atggggagga gtgagacgtc
     1561 gccaaatcgc ccccggaact ctgtctgacc ctcgaatccg atcaggcaat tccgccggcc
     1621 gaatcgacga caccgattcc cgcgcatgag tctagacacc ggtattgagc aacgaactcg
     1681 taggttttct tgtaagtcgc ccggatctcg gcccagagcg aagtgacgtc tggaaacatg
     1741 ttcgttgcga tgtcgtgaaa agccacgtag cgtgtgcaag agcgggcaaa ctcccagtcg
     1801 gcgcggacac ttgtcccaaa gtgctcaccg tcgatgaacg tcaagccgat gcccctctct
     1861 gctgcaacca gttcgcgcag ctcttgagat gagcgatttc cggcgacaaa gtgggtaccg
     1921 gcggtccggc gcacgtagtc gtggacgcgc ggcgaaaggt aaggaccaac cgcgatcgcc
     1981 aagctgagcg ggtggaatcg ccgcaagtat tccactgtcg tgatgaacga tccaccttca
     2041 ttgacaccga tttccacgta ggaacggatc ggaggctgtc ttgcgagcca gacaagatac
     2101 ggagagaact ggttcggcca ctgccatatc gacaaaccct ttcccgagta ttggagcagc
     2161 tcgtccggta aggcggccgc ggtccgttcg tgcaaaccga gcccaagcag gagtcgttcg
     2221 acacctgaag ggtttcgcaa cactttcaac ggtgcctgtc ggatccgttg gatcagcggc
     2281 cagttccaga ccggaggcga gtcgaggatg taattcgccc aagcgccgag cggaacgttt
     2341 ccagcgccgt gaaccagaca cggtcgcgcg ccggtctcgc gattcaggat gcgccggcct
     2401 tccagcaaca ggtccctgtt cccactgccg actgtcgagt cgacttgatc gagaacttgg
     2461 aaaaggcggc attctccatc gaggacaata tcggatagcc tgtttacgta taaatcgatc
     2521 cacgcagctt ggtcgtcgtt gaagatatgc cgcccatggg cgatctccgg cggctttcct
     2581 tcccaatgcc gttcgcgagc ctgcatcgta tcccaagcgg aaagccacgc ttcggctgtg
     2641 gcgaaaatgc tgccactatt gagatatcgt gacgacgttc cggtttccgg agagtgccca
     2701 cccagaatgg gcagcggcca gttgttgcgt tgcgcagaga tgacgatgct cttggggaaa
     2761 gagcgatact tataaaacat ctctcgtagc gtggcgcaga agatcacgtc gctaaaatcg
     2821 accatcaaca cgtatcgata ttcgggatgc tcgcgaagat actccgctac cagggcgatc
     2881 ttttgtcgac cattcggata ggtttgctct tgtcttaaga ctgtcaattg agtgccgcaa
     2941 cggcgagcgc tctcgatcaa ccaccacgac gactgggggg agtgatcgta cgtggctgga
     3001 ctggccacca gtatcctgtg ttcgtcttga aaagacagcg gagttcgtct tggtcgcgcc
     3061 ctggtcgacc gctctgaatc ggactaactg tgcaccaaat cgtcactccg gccgggaccg
     3121 gtggccacaa ggccggcaag ccgatgcagt tcgtggaccc gctccaagag cgtccctcgc
     3181 tcgcggagca aatgtcgaat gaagcaacca tcgtagttaa atagattata ctcgtgattt
     3241 gtataacatt ttaa
//
LOCUS       DATAQL010000868         6159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2382921, whole genome shotgun sequence.
ACCESSION   DATAQL010000868 DATAQL010000000
VERSION     DATAQL010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382921"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..644)
                     /locus_tag="VHS97_15050"
     CDS             complement(237..644)
                     /locus_tag="VHS97_15050"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX3449571.1"
                     /translation="MFRVTREIEFCYGHRLLNYDGKCRHLHGHNGRAVITLEGPELDS
                     RGMLVDFGEIKHKVQHWIDEVLDHNMLLCRDDPLLPLLRERGERVFEMDSNPTAENIA
                     RLIYEHAKEAGLPVVEVLLWETEHCFSTYSQPR"
     gene            complement(743..1447)
                     /locus_tag="VHS97_15055"
     CDS             complement(743..1447)
                     /locus_tag="VHS97_15055"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX3449572.1"
                     /translation="MSRTHDQTCAVLISGGIDSAILCIELLHDFSRVIPLYIRSGLRW
                     EEAELASLDRFLSAVGSTGIESLVVLDEPIADVYGSHWSTNGPNIPGRDSADEAVYLP
                     GRNLLLTVKAAIWCRLREIETLALGSLGSNPFPDCTPAFFQSLETLLNQAMDGKLRLI
                     RPFDQLHKTDVLVRGKLLPLQLTFSCINPVDGRHCGICNKCAERRKGFRDAGLPDLTA
                     YDDDLVHAQRSPAGNR"
     gene            complement(1647..2996)
                     /locus_tag="VHS97_15060"
     CDS             complement(1647..2996)
                     /locus_tag="VHS97_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX3449573.1"
                     /translation="MTDSESCREFALDVVKRLQRAGYTALWAGGCVRDLILGQTPADY
                     DVATNATPEQVMAALPYRALTVGISFGVVRVRHPRTSGVEVEIATFRSDGAYVDGRRP
                     ESVVFSSPELDAARRDFTINGMFLDPLTGEVIDYVGGQADLKNHVLRAIGEPEARLRE
                     DRLRVLRAIRLAARFQFQIEPATRAALRAMSGSVESVSKERIAQELRRMLVHESRAQA
                     IALALDIGLVAAILPDLLLMKGMFQGKPIQPEGDLWDHAMLVLTLLPSDPSFTLAFAA
                     LLHDVGKPRTRHYHEGGYSFHHHEKVGARISDKLCRDLKLSNIEREQISWLVEFHQYL
                     GEAKKLRESKLKRILAEPGIEDLLALHRADALASTGNTEHIDYCEYYLKIEPAGPINP
                     PPLITGHDLVRLGLQPGAHFATILEQVREAQLEGQIQSKRAALEWVDSRRSEWTGGN"
     gene            complement(2989..3645)
                     /gene="rlmJ"
                     /locus_tag="VHS97_15065"
     CDS             complement(2989..3645)
                     /gene="rlmJ"
                     /locus_tag="VHS97_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX3449574.1"
                     /translation="MRIIAGHRRGHKIDGPRASARTRPTSDLVRESLFNILGDLVVDR
                     TVFDLFAGTGALGLEALSRGAERAVFVEKDRECVGLIIRNVATLRYEDRATVKLADVY
                     RWARAFQPVDERPLVVLIDPPYQEYERRGKILNQMIVQLVDKLPAGSVVVLEAGRILD
                     RSILEDLERWDIRRYSSTQIAIKVIADAPVDSGSGPVGTADPSRIDRDGGGSEVDLDD
                     "
     gene            3938..4507
                     /locus_tag="VHS97_15070"
     CDS             3938..4507
                     /locus_tag="VHS97_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3449575.1"
                     /translation="MAHPTDSSALAPERFRMYLRVLAELQLGRGPDVRIDPSDIVQQT
                     LLDAHRDRDHFRGRTESEMAAWLRRLLSCNLTDAARAIGRAKRDVGRECSFEAAVDAS
                     SARLESWLAAQQSSPSERAERNEAILRLLDALALLPEANRQALLLRHCQGLSLAEISA
                     RLGRTPPAVAGLLKRGLAELRSLLPDERG"
     gene            4515..>6159
                     /locus_tag="VHS97_15075"
     CDS             4515..>6159
                     /locus_tag="VHS97_15075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449576.1"
                     /translation="MPDRGPEESDRERRLDEIIAAFLVAEDQGQPLALEAIMVQHPDL
                     VDDLHAFFKEHHRIGSLVAPLRAAASAESGAAETTQVDAKSRPVGSRQPEDLRYESTE
                     ISYATDEPSTKWDDDPNATDPGGPYTTVDSDPIEVGTRVRYFGDYVLLKVLGKGGMGV
                     VYKARQISLNRPVALKMLQAGILATDDDLRRFQNEAEAVAMLDHPRIVPILEVGQYHE
                     QRYFSMKLIGGPSLDRMIGQFTTDPKTAARLVRTVAEAVHHAHQRGILHRDLKPGNVI
                     LDDCGEPHVTDFGLAKRVQADSELTHTGAILGTPAYMAPEQASGKRGMVTTATDVYGL
                     GAILYALLTGRAPFGGESPVGTLEQVREHKPELPSKLNPRVPRDLETITLKCLEKEPA
                     RRYVSAQALADDLRRYLSGESILARPVWLATEAWMWCKRKPALAGLASALILALLIVL
                     AIEAARQRETQVRKAAETNFKRAQTAVEDYLTSVSENTLLKEQDSVDIRGLRKELLKN
                     TLKYYERFADERSNDATPQELLANAYFRIGEVAQEIEPQQ"
BASE COUNT         1279 a         1911 c         1829 g         1140 t
ORIGIN      
        1 ccgtcctcaa ggggaggctg aagggcaaga cgtacgaatg gtgggtcgac gacttaaggt
       61 acccgtacac gccccagagt ttcacggccc ctacgaaagc gcctgacgac ggccacgacc
      121 acgcacactg atcgatcctc acgtgcgcag gtaccggaag ccgccgcggg tcgccgtacc
      181 agacggcaat ccgcggcttt tccaattggg cggtcaagag aaccgatagt cgcgcatcaa
      241 cggggctggg aatacgtcga gaaacagtgc tcggtctccc agagcaagac ctcgacgacc
      301 gggagaccgg cctccttggc atgctcgtaa atgagccgcg cgatattctc cgctgtggga
      361 ttcgagtcca tctcgaatac acgttcgcca cgttccctca agagcggcag cagcgggtcg
      421 tcgcgacaga gcagcatatt gtggtcgagc acttcgtcga tccagtgctg gactttatgc
      481 ttgatctcac caaaatccac cagcatgcca cgtgagtcga gctccggtcc ctccagagtg
      541 atcaccgccc gcccgttgtg cccgtggagg tgcctacact tgccgtcata atttagtaag
      601 cgatgcccgt aacaaaactc aatttcgcgt gttacccgaa acatgatgat gtaaaccagc
      661 cgttaaccgt gggtcattat tgtgacgcgt gataggcttt gttggttctt cacgctgcgc
      721 ccaataggcc tcattgccgc agtcaacgat tgccggccgg cgatctctgg gcgtgcacca
      781 ggtcgtcgtc gtacgccgtc aagtcaggca gcccggcgtc acgaaagccc ttcctgcgct
      841 cagcacattt gttgcagatc ccgcaatgtc ggccatcgac cggattgatg caagaaaacg
      901 tcagctgaag cggtaagagt ttgccgcgga cgaggacgtc cgttttgtgc aattggtcga
      961 acgggcgaat aagccgcagc tttccgtcca tcgcctgatt cagcagcgtc tcaaggctct
     1021 gaaagaacgc gggcgtgcag tccggaaacg ggttcgatcc cagagaaccc agtgccagag
     1081 tttcgatctc gcggagccgg caccagatcg ccgctttgac tgtaagaagc aggttgcgac
     1141 cgggaagata gaccgcttca tcggctgaat cgcggccggg gatgttcggc ccattcgtgc
     1201 tccagtgcga gccataaacg tcggcgatcg gttcgtcgag gacaacgagc gattcgatcc
     1261 ctgtgcttcc aacggcgctg agaaagcggt ctaacgacgc cagttcagcc tcttcccatc
     1321 gcagacccga tcgaatgtag aggggaatga cgcgcgaaaa gtcatggagc agctcgatgc
     1381 aaagaatcgc gctatcgatt cctccactga tcaagacggc gcacgtttgg tcgtgagttc
     1441 gagacatcag gtccagccgg tgaatccggg atcagggctc ccacgtggtg cttcgatccg
     1501 cggctcagac attctaggcc tgggcccaat cgccggcaac cagactgaac aggcgccaag
     1561 gtttcagcca tgacgccgac cgaggcgccc gatcgagtta gtcgattcga ggtgccgtga
     1621 gtcgatcttc tgcatcaaca agctcgctaa ttcccgccgg tccattctga gcgccggctg
     1681 tcgacccact cgagcgccgc tcgcttgctc tgaatctggc cctcgagttg tgcctcgcgg
     1741 acctgttcca ggatcgtggc gaagtgggct cccggctgaa gaccgagacg gaccaggtcg
     1801 tgcccggtga tcaagggggg cggattgatc gggccggccg gctcgatttt caagtagtac
     1861 tcacagtagt caatatgctc ggtgttgccc gtcgacgcca gcgcgtcggc gcgatgaagc
     1921 gccaacaggt cttcgatacc cggctccgcc aaaatgcgct tgagcttcga ctcgcgaagt
     1981 ttcttggctt cacccagata ttgatgaaac tcgacgagcc agctaatttg ctcccgctcg
     2041 atgttggaga gcttcaaatc gcggcacaac ttgtcactga tgcgtgcgcc gaccttttca
     2101 tgatgatgaa aactgtaccc accctcgtga tagtgccggg ttcgtggttt gcccacgtcg
     2161 tgcaacaggg ccgcgaaggc gagcgtaaag cttggatcgg agggcaaaag ggtcagcacc
     2221 agcatcgcgt ggtcccagag atctccttcg ggttgaatcg gcttgccctg aaacattccc
     2281 ttcatcaaga gcaggtcagg gaggatggcg gctacgagcc cgatgtcgag tgccagcgct
     2341 atggcttggg ctcggctctc gtgcacgagc attcgtcgta attcctgggc gatccgctcc
     2401 ttggagacgc tttcgaccga acctgacatc gccctgagcg cggcgcgtgt ggcgggttcg
     2461 atttgaaact gaaaccgggc cgccaggcga atcgcgcgga gcacgcgcag ccggtcttcg
     2521 cgcagccttg cctcgggctc gccgatagcg cgaagtacgt gattcttcag gtcggcctgg
     2581 cccccgacgt agtcgatcac ctcgcccgtg agcggatcaa gaaacatccc gttgatcgtg
     2641 aagtcgcggc gggcggcgtc gagttcgggt gaactgaaga cgaccgactc cggtctgcga
     2701 ccatcgacat aagcaccgtc gctacggaag gtcgcaattt cgacttcgac gcccgaggtg
     2761 cgtgggtgcc gaacccgcac gacgccgaat gagatgccca ctgtgagagc gcgataaggc
     2821 aaggccgcca tgacctgctc gggggtcgca ttggtcgcca catcgtaatc agcgggcgtc
     2881 tgacccagaa tgaggtcgcg cacacatcca cccgcccaga gcgcggtata tccagcccgc
     2941 tgcagccgct tgacgacatc cagcgcgaat tcgcggcatg attcgctgtc agtcatcgag
     3001 atcaacctcc gaaccgcccc cgtctcgatc aatccgcgat gggtccgcgg tcccaaccgg
     3061 gccggaacct gaatcaaccg gcgcgtcggc gatcaccttg atcgcaatct gtgtactgct
     3121 gtagcggcgg atgtcccagc gctccaaatc ctcgaggatg ctccggtcga gaattcgccc
     3181 cgcctcgagc acgaccacgg atccggcggg caacttatcg acaagctgca cgatcatctg
     3241 gttcaggatc ttcccgcgtc gctcgtattc ctgataggga gggtcaatta atacgacaag
     3301 aggccgctcg tcgaccggtt gaaacgctcg ggcccagcga tagacgtcgg caagcttgac
     3361 tgtggcgcgg tcctcgtaac gaagcgtggc gacgttgcga atgatcaggc cgacacattc
     3421 gcggtctttc tcgacaaaaa ccgcacgctc ggcaccccgg ctcagggcct caagacccaa
     3481 tgcaccggtg ccggcaaaga ggtcgaagac cgtacggtcc acgaccaggt cgcccagaat
     3541 gttaaataac gactcgcgca ccaggtcgct cgtaggccgc gtcctggccg acgctcgcgg
     3601 accatcgatc ttgtggccgc gtctatgccc cgcgataatt cgcatttggc aagtctccgt
     3661 cgtggagaag agctcgacgc cgttccgtcc ggctcgcatc agcagcgtcc tcgacccaca
     3721 ttcgatctta ccccaagccg gaacgaaggg cgaatcttca gttcggagcg tatcagccgc
     3781 cagcatcatc gagcagcgcg gaaatgcaag ctgatcaagg tttaaggtgg cccaagcttg
     3841 ttccgaccaa tgttgaccgg gcgcgaaatc gaaaaaacgt gattcgggcg cgccccgtag
     3901 ccgcgccgat ggcggctact ttctcaggag gattgttgtg gcccatccaa ccgattcgtc
     3961 ggcactggca ccggaacgat tccgcatgta cttgcgggtg cttgccgaac ttcaacttgg
     4021 ccgcggaccg gatgtgagga tcgatccgtc ggacatcgtg caacagacct tgcttgacgc
     4081 gcatcgtgat cgggaccatt tccggggccg gaccgaatcc gaaatggcgg cctggcttcg
     4141 ccgtctccta tcctgcaact tgacggatgc ggcgcgggcg atcgggcggg ccaagcgaga
     4201 tgttggccgc gaatgttcgt tcgaagcggc ggtcgacgcc tcgtcggccc gcctggaatc
     4261 atggctagct gcacagcaat cctccccatc agagcgggcc gagcgaaacg aggcaattct
     4321 tcggctcttg gatgcccttg cgctcttgcc cgaggccaat cgtcaagccc tgctgctgcg
     4381 gcattgccag ggactgtcgc tggccgagat tagcgcgcgc ctgggccgca ctccaccggc
     4441 ggttgccggc ttactcaagc gaggtctagc cgaactccgt agcctgttgc ccgacgaaag
     4501 gggctgaaac cacaatgcca gaccggggac ctgaagaatc cgaccgcgag cgccgccttg
     4561 acgagatcat tgcggctttt ctggttgccg aggatcaggg tcaaccgctc gcacttgaag
     4621 caattatggt tcagcacccc gacctggtcg acgacctaca tgccttcttc aaggagcatc
     4681 atcgcatcgg aagtcttgtg gccccgctcc gcgctgccgc aagcgcagag agtggcgccg
     4741 ctgagacaac gcaggtcgat gcgaaatccc ggccggtcgg gtcgcggcaa cccgaagatc
     4801 tacgctacga gtcgacggaa atctcctatg cgaccgacga gccgtcgacg aagtgggacg
     4861 acgacccgaa cgcgacggac ccgggcggtc catataccac ggtcgactct gacccgatcg
     4921 aagtcgggac gcgcgtccgc tacttcggcg actacgtttt gctcaaagtg ctgggcaaag
     4981 gtgggatggg agtggtctac aaggcccgac agatcagcct taaccggccg gtggcgctca
     5041 agatgctgca ggccggcata ctggcgaccg atgacgacct gaggcgattc cagaacgagg
     5101 ccgaggccgt cgcgatgctc gaccatccgc gcatcgtacc gatcctggaa gtaggccaat
     5161 accacgaaca gcgatacttc agcatgaagc tcatcggcgg gccaagcctc gaccgtatga
     5221 tcggccaatt caccaccgat ccgaaaaccg ccgcgcggct tgtccggacg gtggccgagg
     5281 cggtgcatca tgcgcaccag cgcggaatcc tacatcgcga cctcaagccg ggcaacgtga
     5341 tacttgacga ttgcggtgag ccacacgtga ctgacttcgg cctggccaag cgagttcagg
     5401 ccgacagtga gctgactcac accggcgcga tcctaggcac accggcctac atggctcccg
     5461 agcaagcctc aggcaaacgg ggaatggtga ccactgccac cgacgtctat ggcctggggg
     5521 caatcctcta cgccttgctt accgggcgtg ctccctttgg aggtgagtcg cccgtcggaa
     5581 cgctcgaaca ggtccgtgag cacaaacccg agctcccctc aaagctcaat cctcgcgttc
     5641 cgcgcgatct ggagaccatc acgctcaaat gccttgagaa ggaaccagcg cggcgctacg
     5701 tctcggccca agcactcgcc gacgacctgc ggcggtatct gagcggcgag tccatcctgg
     5761 caaggcccgt gtggttggcc accgaggctt ggatgtggtg caaacgaaaa ccagccctgg
     5821 caggtttagc ctcggccctc attctcgcgc tgttgattgt cctggccatc gaggccgcgc
     5881 gacaacgcga gacacaggtc cgcaaggcag ccgagaccaa tttcaaaagg gcccagaccg
     5941 ccgtcgagga ttatctgacc agcgtcagtg aaaacaccct tttgaaagaa caggattcgg
     6001 tcgacatccg cggcctccgc aaggaattgc tcaaaaacac gctcaaatac tacgaacgtt
     6061 tcgccgacga acggagcaac gacgcgaccc cccaggaact gctagcaaac gcctactttc
     6121 ggattgggga ggtcgctcag gagatcgagc cgcagcagc
//
LOCUS       DATAQL010000869         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4823373, whole genome shotgun sequence.
ACCESSION   DATAQL010000869 DATAQL010000000
VERSION     DATAQL010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4823373"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..433)
                     /locus_tag="VHS97_15080"
                     /pseudo
     CDS             complement(1..433)
                     /locus_tag="VHS97_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611079.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            590..1669
                     /locus_tag="VHS97_15085"
     CDS             590..1669
                     /locus_tag="VHS97_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3449577.1"
                     /translation="MRKGITVTVNAADRTRLEAVVANRNSPQKHAWRCRIVLLTADGL
                     GTAAIMQQTGKSKSVVWRWQERFMHAGVDGLLRDKTRPPRIPPLAQAVIDRVVALTSA
                     DPPGETTHWTAAAMAKATGISVSSVQRIWRAHGLEPHRVRQFKLSNDMAFADKLRKIA
                     GLYVDPPAHAVVLSVDEKSQIQALDRTQPGLPIKKGRCGTMTHDYIRNGTTTLFAALN
                     VLEGKVIGRCMQRHRHQEFIRFLNAVEAEVPAGKIVHVIVDNYATHKHPKVKAWLARH
                     PRFIFHFTPTSCSWLNAVEGFFAKLTRRRLKRGVFRSVVELQAAINRFIAEANHDSKP
                     FVWTADPDKIIAAVNRGHQVLDSIH"
BASE COUNT          339 a          632 c          523 g          306 t
ORIGIN      
        1 cctggcagca agacaaagcg caccagattg ccgagcgcgt cgaccagggc cacgatcttg
       61 gtggtgaggc caccgcgcga gcggcctatg gcctgatttt gagtccccct tttgcgccgg
      121 ttccgtgctg gtggacccgg acgatggtgc cgtcgatgat cgcgtactcg aaatcgggat
      181 cgtcggacaa gctcgcgaaa acccgcgcga aggtcccctt cagggcccat cggcgaaagc
      241 gctggaagac cgagttccag ttgccgaacg ccgcaggcag gtcgcgccac ggcgatcccg
      301 tgcgggcgat ccacaggacc gcttccaaga acagccggtt gtcctctccc gaccgacccg
      361 gatcaccggg cttgccggcg catagcggcg caacccgttc ccactgcctg tcactcaaga
      421 tgctccgctt caagactgac cccctcaggt ttccagtctt gaatccgatt tcgagtccgg
      481 ggggaatccc cttcgttgcc cagaatgtca acagccccta gtggatccga cctgacgaat
      541 gagtcccttg ggggattctc cggcgcccgc ggccgtgcga gtctcgggaa tgcgcaaggg
      601 aatcaccgtc acagtcaacg ccgccgaccg cacccgcctg gaagcggtgg tggccaaccg
      661 caacagtccg cagaagcatg cctggcgctg ccggatcgta ttgttgaccg ccgacggcct
      721 cgggacggcg gcgatcatgc agcaaaccgg caagagcaag tccgtggtct ggcgctggca
      781 ggaacgcttc atgcatgccg gcgtcgacgg cctgctgcgc gacaagacgc gtccgccacg
      841 catcccgccg ctggcccagg cggtcatcga tcgggtcgta gccctgacca gtgccgatcc
      901 gccgggggaa acaacccatt ggacggcggc cgccatggcc aaggcgaccg gcatcagcgt
      961 cagctcggtg cagcgcatct ggcgtgccca tgggctcgag ccgcaccgcg tccgccagtt
     1021 caagctctcc aacgacatgg cctttgccga caagctcaga aagatcgctg gcctctatgt
     1081 cgatccgccg gcccacgccg tcgtgctctc ggtcgatgaa aagagccaga tccaagccct
     1141 cgaccggacc cagccggggc tgccgatcaa gaaggggcgc tgcggcacca tgacccacga
     1201 ctacatccgc aacggcacca cgaccctctt cgccgccctc aacgtgctcg agggcaaggt
     1261 catcggccgc tgcatgcagc gccaccggca tcaggaattc atccgcttcc tcaatgccgt
     1321 cgaggccgag gtgcctgccg gaaagatcgt ccatgtcatc gtcgacaact acgccaccca
     1381 caagcatccg aaggtcaagg cctggctggc ccgccatccg cgcttcatct tccatttcac
     1441 ccccacctct tgctcctggc tcaatgccgt cgagggcttc ttcgccaagc tcaccaggcg
     1501 tcgcctcaag cgcggcgtct ttcgctccgt ggtcgagctt caggccgcca tcaaccgctt
     1561 catcgccgag gccaatcatg actccaagcc cttcgtctgg accgccgacc ccgacaaaat
     1621 catcgctgcc gtcaatcgag ggcaccaagt gttagattcg atccactagc ttcgcgagtg
     1681 atcgctcgaa ttgcttcgat ccgcgacagc agatcctcga cgccttccat ttggtgccga
     1741 gttgatatgg ttggcacagc agggtgatgc ccgaagcgac accgaacgcg ccgctggcag
//
LOCUS       DATAQL010000870         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1771722, whole genome shotgun sequence.
ACCESSION   DATAQL010000870 DATAQL010000000
VERSION     DATAQL010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771722"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2163)
                     /locus_tag="VHS97_15090"
     CDS             complement(<1..2163)
                     /locus_tag="VHS97_15090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3449578.1"
                     /translation="MSGDLSGFSMMELFRMEAESHTATLSAGLVALEGAPATQHAIEP
                     LMRAAHSLKGAARIVGLEAAVQVAHALEDCLVIAQQGKLVLRPEHIDILLQGVDLLIQ
                     IAQIDESDIEAWQSGHTAEIDALTAALVGVKEGQVLPRDSPSSSNRETTSLPHDLDVS
                     ELTSSSSILPDHSQAGVGTVDEGIPREQERDQEAVLPPSFTTTDNDRARAHTTVESAV
                     SVIDRDRVVRVTAESLTRLMGLAGEALVQTHRLPALVASLWRLKGRHTGLLESLQRLE
                     DRLSSQQVTMSGAARDLLANAKAQVTQDLSELGDAVLAIEEFARVSEDVSSRLHHEVL
                     ASRMRPLADGVRGFPRLVRDLARELGKQARFEIAGETTGVDRDILDRLEAPLNHLIRN
                     ALDHGIESPDDRRASGKSPVGTIRLEARHRAGMLQIVLADDGRGIDLNRLRTKVVERG
                     LTTAPVAARLNETELLDFLFLPGFSTKEQVTEVSGRGVGLDVVQSMVHTVRGSVRVAS
                     RSGKGTRFVLQLPITVSVIRALLVEVAGEPYAFPLNRIDRIFMLDRNDLRELEGKQHA
                     MLDDQPIGVVEAARIFDLPAGIVPRADKLLPVVVASDRSHRFGVVVDRFLGERDLRVS
                     PLDARLGKVPNINSSSVLENGWPVLIVDVEDLIRSIDNMLSGRRLGRLAAQEVANQVR
                     AAKRILVVDDSITVRELERQLLENRGYTVDVAVDGVDGW"
     gene            complement(2160..2903)
                     /locus_tag="VHS97_15095"
     CDS             complement(2160..2903)
                     /locus_tag="VHS97_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3449579.1"
                     /translation="METNPLATNPGSGNALSSRKPGEGDCWNYIGVSGDQSCPELKTF
                     IHCRNCHVFAAAARTFFDRPAPEGYLAGWSRWLIGSAEQDARVEGNGDDQNGTQFHGE
                     SVSLLIFRLGAEWLAFRAHTVAEVTNPRPVHHVPHRSNETFVGLVNLQGQVQLCVSLH
                     GLLGVTAPSAPTRLIVLRDGDKGETWAFPADEVLGVENVPRNRLRSVPSTLANPTVGF
                     SQAVLSWNERSIGLIDEQRVFATLRSLGA"
     gene            complement(2908..4209)
                     /locus_tag="VHS97_15100"
     CDS             complement(2908..4209)
                     /locus_tag="VHS97_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX3449580.1"
                     /translation="MAALMCEIEGLLASRLGLDPVSVGPKQIARAVQQRMQELGLADV
                     GEYESWVRKSESELQALIEEVVVSESWFFRDERPFQYFRDYVRERWLNDPLRPPLRVL
                     SLACAGGEEPYSIAIGLRELGLTAQRFHIDAVDISARRLEIARRGVYSANAFRGSDLG
                     YRARHFREHPQGYELDALIRGTVTFHQASALDLRPLKASTPYDVIFCRNLLIYLEARA
                     RLCVMASIDKRLAPDGLLFIGHADRLDVLGDEPKFVAVGHPGCFAYCHKARGDACSNS
                     LPLKPPLAAEIIKSAGTAAGPAPVRLPPADPTAMQSFQPDATAIGSRQINVPPLLDQA
                     AELANKGRFDEAVATCEQHLQSQGLSPSAYYLMGMICQAAGNRQRAEDCFHKTVYLDP
                     LHDEALLTLALLAERRGDHAAAAGFHRRAERSVTSSGKRVK"
     gene            complement(4258..4791)
                     /locus_tag="VHS97_15105"
     CDS             complement(4258..4791)
                     /locus_tag="VHS97_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3449581.1"
                     /translation="MLLLAFTAGPNRYAIDVARIVEIVPRVELRSVPHAPVFLAGLLG
                     YRGHVVPVIDLCLLLDVAPCRDCLSTRIILVSVAPDDHNQSKPDRGDSGEAIGGSRPD
                     PVRPTDLLGLIAERVSDLTFAKPEQLMPVPVSLPQAPYLGDVVQTDQGIVQLIAVERV
                     PDATLRSSSSIEVAALD"
BASE COUNT         1025 a         1445 c         1436 g          910 t
ORIGIN      
        1 ccagccgtca acgccatcga cggcgacatc aacagtgtaa ccgcggtttt ccagcaactg
       61 cctttctagc tcacggactg tgatagaatc gtcgacgacc agaattctct tggcggcgcg
      121 aacttgattg gcaacttcct gagcggcaag cctgccgagg cggcgaccag aaagcatatt
      181 gtcgatcgag cgaatcaaat cctcgacgtc gacgatcaac accggccatc cgttttcgag
      241 caccgaggag ctattgatat tgggcacctt gcccagccgc gcatccagcg gcgaaacgcg
      301 caggtcacgt tcgcccaaga agcgatcgac gaccacacca aaacggtggc tccgatcgct
      361 ggcaaccact acaggcagaa gcttgtcggc gcggggaact atgccagcgg gcaggtcgaa
      421 tattctggct gcttcaacca cgcctatggg ctggtcgtcg agcatcgcgt gttgcttacc
      481 ctcgagctcg cgcaggtcgt tgcggtcgag catgaaaatg cggtcgatcc ggttcaacgg
      541 gaaagcgtac ggctctccag cgacttcaac gagcaaggca cggatgacgg atacggtgat
      601 tggtaactgg agtacaaacc gcgttccctt cccggagcgg ctagctactc gaaccgagcc
      661 gcgcaccgtg tggaccatgc tctgaaccac gtcaagcccg acgccccggc cggagacctc
      721 ggtgacctgc tccttggtcg agaatccagg gaggaaaaga aagtcaagca actcggtctc
      781 gttgagccgg gcagccaccg gggcagtcgt caggccgcgt tcgacgacct tcgtacgaag
      841 ccggtttaag tcaattccgc ggccatcgtc ggcaagaacg atttgcagca tacccgcacg
      901 atgtcgtgcc tccaggcgga ttgtcccaac cggggacttg ccagatgcgc gacggtcgtc
      961 aggcgactcg ataccgtgat ccagggcatt gcgtatgaga tgattgagcg gcgcctcgag
     1021 ccgatcgaga atatcgcgat caacgcccgt cgtctcgccg gcgatctcaa accgtgcctg
     1081 cttgccgagc tcacgagcca ggtcacggac caggcgcggg aagccgcgca caccatcggc
     1141 taatggccgc atcctgctag caagtacttc atggtgcagc cggctggaaa cgtcctcgct
     1201 gacgcgggca aattcctcga tcgccaacac cgcgtcgcct agctcgctca agtcctgggt
     1261 gacctgggcc ttggcattgg cgagcagatc gcgagcggcg ccggacatcg taacttgttg
     1321 actcgacaga cggtcttcga ggcgctgcaa tgattccaga agacccgtgt gccgtccttt
     1381 gagccgccag agcgaggcaa ccaacgcagg gagtcgatga gtttgcacca aagcctcgcc
     1441 cgcgaggccc atcaatcgcg tcaagctctc agctgtcacg cgcacgacgc ggtcacgatc
     1501 gatgactgag accgcgctct cgaccgtggt gtgcgcgcga gctcgatcat tgtcagtggt
     1561 tgtgaacgag ggaggtaaaa ccgcctcctg atcgcgttcc tgctctcgag gtattccctc
     1621 gtcgaccgtt ccgaccccgg cctggctatg atcggggagg atggaactcg atgaagtaag
     1681 ctcggaaaca tccagatcat gaggaaggga cgtcgtctca cggttggagg aagaagggct
     1741 gtcccgcggc agcacttggc cctccttgac gcccactagc gcggccgtca gtgcatcgat
     1801 ctcggccgtg tgcccggatt gccaggcctc gatgtccgac tcgtcgatct gtgcgatctg
     1861 aatgagcaga tcaacacctt gaagcaggat gtcgatatgc tcgggccgca gcacgagctt
     1921 cccttgttgt gcgatcacca agcagtcttc cagcgcatgg gcgacctgta cggcggcttc
     1981 gagaccaacg atgcgtgccg cccccttcag cgagtgtgcc gcgcgcatga gcggctcaat
     2041 tgcgtgctga gtggccggag caccctcaag cgcgaccagg cccgccgaga gcgtggccgt
     2101 atggctctcg gcttccatgc ggaagagctc catcatcgag aatccgctga gatcgccgct
     2161 cacgcgccca gactccgcaa agtcgcaaag acccgctgtt cgtcgatcaa gccgatgctc
     2221 cgctcattcc aggacaagac ggcttggcta aacccaacgg ttgggttcgc cagtgtcgat
     2281 ggaacgctgc gtaaccggtt gcgcggaacg ttctcaactc ccaagacctc atcagccgga
     2341 aatgcccatg tctcgccctt gtctccgtca cggagcacga taagccgtgt gggcgccgat
     2401 ggagccgtca cgccgagcaa tccgtgtaac gacacgcaca gttgtacctg gccttgcaga
     2461 tttaccaatc cgacgaaagt ttcgttggag cgatggggta cgtgatgtac gggccgaggg
     2521 ttggtaactt cggcgacagt gtgtgcacga aaggccagcc attccgctcc caagcgaaag
     2581 atcaagagac ttaccgattc gccgtgaaac tgggtgccgt tctggtcgtc cccgtttcct
     2641 tcgacgcgcg catcttgttc tgccgagccg atgagccacc gggaccagcc ggccagatag
     2701 ccctcgggtg cagggcggtc gaaaaaggtc cgcgccgccg cggcgaacac atggcagttt
     2761 cgacaatgaa tgaacgtttt tagctcgggg cagctctgat cgccagatac gccgatatag
     2821 ttccagcagt caccttcacc gggcttgcgg ctagagagag cgtttcccga gccaggattg
     2881 gttgcgagcg ggtttgtctc catattatta ctttactcgt ttcccggagc tggtcactga
     2941 tcgctcggcg cggcgatgaa agccagcagc tgcggcatga tcgccacggc gttcggccaa
     3001 gagcgcgagg gtcaaaagcg cttcatcatg caagggatcg aggtacacgg tcttgtggaa
     3061 acagtcctcg gcccgttgac gattgccggc cgcttggcag atcattccca tcaaataata
     3121 ggccgacggc gacagtcctt gagactgaag gtgctgttcg caggtcgcga cggcctcgtc
     3181 aaaccgcccc ttgttggcca attctgcggc ttggtctagc aacggaggca cgttgatttg
     3241 gcgagagccg atcgctgtcg cgtctggttg aaaagactgc atcgcggttg ggtcggcagg
     3301 aggcagacgg actggagcag gtccggctgc cgttcctgcc gatttgatga tttcggccgc
     3361 caaaggcggt ttgagcggca aggaatttga gcaagcgtcg ccacgggctt tatggcaata
     3421 agcaaagcaa cccggatggc caaccgcgac aaattttggc tcgtctccga gcacgtcgag
     3481 ccggtcagca tggccgatga aaaggaggcc atcgggcgcc agccgcttgt cgatcgacgc
     3541 catgacgcac agtcgcgccc ttgcttcaag gtaaattaag aggttccgac agaaaatgac
     3601 gtcgtaagga gtcgacgcct tgagcggacg aaggtcgagc gcactggcct ggtgaaatgt
     3661 aactgtaccg cgaatcaagg catcgagttc gtaaccttgt ggatgctcgc ggaaatgccg
     3721 cgcacgataa ccgagatcgg aaccgcggaa ggcatttgcc gagtaaactc cgcggcgagc
     3781 gatctccagt cgtcgggcgc ttatgtcaac ggcatcgata tgaaaccgtt gtgcggtcag
     3841 tccgagctcg cgcagcccga tcgcaatcga gtatggttct tcgcctccag cgcatgcgag
     3901 gctgagaact ctaagcggcg gacggagcgg atcgttaagc cagcgctcgc gcacgtagtc
     3961 tcgaaaatac tggaacggac gctcatcgcg gaaaaaccag ctctccgaga ccacgacttc
     4021 ctcgatcaac gcctgtaatt ccgactccga cttacgcacc cagctctcgt attcgcccac
     4081 atcggccaga ccgagttcct gcatacgctg ctgcaccgcg cgcgcgatct gtttcggtcc
     4141 caccgagacc gggtctagtc ccagcctgct cgccagcaac ccctcgatct cgcacataag
     4201 agccgccatt gcgattccgc tttcagattc tggtggtcga tgttagacgt cgcatcccta
     4261 atccagtgca gcaacctcga tcgaggacga ggaccgtagc gtggcgtcag gaaccctttc
     4321 aaccgcgatt aattgaacga ttccctgatc ggtctgaacg acgtcaccca ggtagggcgc
     4381 ctggggcagg gacaccggaa cgggcatcag ctgctcgggc tttgcgaagg tcaaatcact
     4441 gacacgctcg gcgatcagac ctagtaaatc tgtaggtcga actgggtcag gccgcgagcc
     4501 gccgattgct tcgccggagt cacctcgatc tggcttcgat tgattgtgat catcgggggc
     4561 gacactaacc aggatgatgc gggtgctgag acaatcccga catggggcaa catcgagcaa
     4621 caggcaaaga tcgatcaccg gaacgacatg gccgcgatag ccgaggagcc ctgccaggaa
     4681 cacaggtgca tgaggtaccg agcggagctc gaccctgggt acgatctcga caatccgcgc
     4741 cacgtcaatc gcgtagcgat tggggccagc agtgaaggcc aagagcagca tgaattgtac
     4801 gccaagccat attcag
//
LOCUS       DATAQL010000871         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_90420, whole genome shotgun sequence.
ACCESSION   DATAQL010000871 DATAQL010000000
VERSION     DATAQL010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90420"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VHS97_15110"
     CDS             complement(<1..1297)
                     /locus_tag="VHS97_15110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449582.1"
                     /translation="MPVDSKRVQAVFLMAVEHEDDADRASVLDRECATDLELRQRVLA
                     LLRSHDEPDRFLDQPIVRSTPPLWANLSRFDGALASDSSADLTRGVPPAPPGESPPRP
                     SAKSAASNATTESAGSCIGPYKLLQKIGAGGMGAVYMAEQEQPVRRWVALKIIKPGMD
                     SEHVIARFEAERQALALMDHQNIARVFDAGTTDTGRPFFVMELVHGMSITEHCDQSQL
                     TTRERLELFIPVCQAIQHAHQNGIIHRDIKPSNVLVTLYDGRPVAKVIDFGVAKAIEQ
                     RLTTRTLFTQCGSIVGTYEYMSPEQAEMTAPGADTRSDIYSLGVVLYELLTGTTPLDR
                     EKMVGSTHTDILRRIREEEPPKPSTRLKGSKIALPMISAQRKSEPQRLTQLMRGELDW
                     IVMKALEKDRARRYQTASDFGRDIERYLNGDVVEACPPSA"
     gene            complement(1376..1924)
                     /locus_tag="VHS97_15115"
     CDS             complement(1376..1924)
                     /locus_tag="VHS97_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3449583.1"
                     /translation="MTDVTRILSAIGQGVPESAEQLLPLVYEELHRLAVQRLGQEAPG
                     HTLQATALVHEAYLRLVDVEQAQVWDSRHHFFAAAALAMRRILVESARRKARLKRGGR
                     LRRVDLFDHAAPDPDDDLLALDAALNGLMVEDPIAAKVVELRQFAGLGHEEIAATLGI
                     TVYLARQKWTYARAWLRDALEG"
     gene            complement(1961..2677)
                     /locus_tag="VHS97_15120"
     CDS             complement(1961..2677)
                     /locus_tag="VHS97_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX3449584.1"
                     /translation="MRSQYHSSRREFVRLTGLAAGAAGLAFPLNGRAESQPAAARDPD
                     AVLARLIEGNKRFVGGQLVHPGRTPADFAALAEGQAPLAIIVGCADSRVAPELVFDQG
                     VGDLFVVRVAGNIVSGAGPTVKGSIEFAVAELGARLIMVLGHTQCGAVKAAIAHIDAN
                     DSLPGSIGELIDPIRPAVRAVSGQTGDKLENVTNANIAQGVERLKTLDPIVSKFAKTG
                     ELKVVGAVYELRTGAVKLLG"
BASE COUNT          483 a          853 c          848 g          534 t
ORIGIN      
        1 aagccgacgg cgggcaggcc tcgacgacgt cgccattcag gtagcgctcg atatcgcgac
       61 cgaagtcgct cgctgtttgg tagcgcctgg cacgatcctt ctcgagtgcc ttcatcacga
      121 tccagtcgag ctcaccacgc atcagctgcg tcagccgctg gggctccgac ttgcgctggg
      181 ctgagatcat cggaagcgca atcttcgacc ccttcaagcg tgtgctgggc ttggggggct
      241 cttcctcgcg gatgcggcgc aggatgtcgg tatgggtcga tccgaccatt ttctcgcggt
      301 ctaacggcgt ggttcctgtg agcagttcat acaagaccac gcccagggag taaatgtcgc
      361 tgcgggtgtc tgcgcctggt gcggtcatct cggcctgctc gggactcatg tattcgtacg
      421 tgccgacgat cgagccgcat tgcgtgaaca gggtccgtgt ggtcaggcgt tgctcgatgg
      481 ccttggccac accgaagtca atcaccttgg cgacgggcct gccgtcgtaa agtgtgacca
      541 ggacgttcga aggtttgatg tcgcggtgga tgatcccgtt ctggtgagcg tgttgaatcg
      601 cctggcaaac cgggataaat agctccagac gctctctcgt cgtaagctga ctctggtcgc
      661 aatgctcggt gatcgacatt ccatgcacca gctccatcac gaagaaaggc cggccggtgt
      721 cggtcgtccc agcatcgaag acacgcgcga tgttctgatg atccatcaga gccagggcct
      781 ggcgctcggc ctcgaaccgg gcgatgacgt gctccgagtc cattccgggt ttgatgatct
      841 tgagcgcgac ccaccgtcgg accggctgct cttgctcggc catataaaca gcgcccatgc
      901 cccccgcgcc gatcttttgg agcaacttgt acgggccgat acaactgccg gcactctcgg
      961 tcgtggcatt tgatgcggcg ctttttgcgc tcggtcgcgg cggcgattcg ccggggggcg
     1021 cggggggcac accccgcgta agatcggcac tggaatcgga tgctaaggcg ccatcgaatc
     1081 gagaaaggtt cgcccacaac ggcggggtgg aacggacgat cggctgatcg aggaaacgat
     1141 ctggttcatc gtgagaccgg agcagtgcta gaacccgttg ccgcagctcc aaatcggtcg
     1201 cgcattcgcg gtctagcaca gacgcgcgat cggcgtcatc ttcatgttca accgccatca
     1261 ggaatacggc ctgtacgcgt ttcgagtcga ccggcatcgg atgctcccaa ggtcggaatc
     1321 acctttatcc atgcgccgtt cgaagccgaa cctgtcgaaa aatcttcaag gttttttagc
     1381 cctcgagggc atcgcggagc caggcccgcg cgtaggtcca tttctggcga gcgaggtaca
     1441 cggtgatgcc gagagtggcc gcgatttcct cgtgccctaa cccggcaaac tggcggagct
     1501 cgaccacttt ggccgcgatc gggtcttcga ccatcagccc gttaagggcc gcgtcgagag
     1561 caagcaggtc gtcgtccggg tcaggggcgg cgtggtcgaa aaggtcgact cgacggagcc
     1621 gaccgccccg ctttagacgg gccttacgcc gcgctgactc gaccaggatc cgccgcattg
     1681 cgagagccgc cgcggcgaag aagtggtggc gactgtccca gacctgcgcc tgctcgacgt
     1741 ctacgagccg caggtaggct tcatgaacga gcgccgttgc ctggagcgtg tgtccgggcg
     1801 cctcttgtcc cagcctctgg acggcaagtc gatggagttc ctcatagacc aagggcagca
     1861 attgttccgc ggactcgggt acgccctgcc cgatcgccga gagaatccgc gtgacgtctg
     1921 tcatcaatgg aagtccccag ggtcttccga gaccaacaga tcatccgagc agtttgaccg
     1981 cgccggtgcg cagttcatat accgcgccga ccacctttaa ttcaccggtc ttggcgaact
     2041 tggaaacgat cggatcgaga gttttgagcc gctcgacgcc ttgtgcaatg ttggcgttcg
     2101 tcacgttctc gagcttgtca cccgtctggc cggacaccgc gcgtaccgcc ggccggatcg
     2161 ggtcgatgag ctcaccgatc gatcctggta gtgaatcgtt ggcgtcaatg tgcgcgatgg
     2221 cggccttgac cgcgccgcac tgagtgtggc ccagcaccat gatgagccgc gcacccagct
     2281 cggcgaccgc aaactcgatg ctccccttca ccgtggggcc cgcaccgctg acgatgttcc
     2341 ccgccactcg caccacaaac aggtcgccga cgccctggtc aaatacgagc tcgggtgcca
     2401 cgcgcgagtc ggcgcacccg acgatgatgg ccagaggcgc ctgcccctcg gccaaggctg
     2461 cgaagtctgc cggcgtccgt cctggatgga caagctggcc gcccacgaat cgcttgtttc
     2521 cctcgatcag gcgggcgagt acggcgtcgg ggtcgcgtgc cgcggccggc tggctctctg
     2581 cccgtccgtt caatggaaaa gccagcccgg ccgctccggc agccagtccc gtgagccgta
     2641 caaactctcg gcgagacgaa tggtattgtg atcgcattac caatccttcc gatgaacggg
     2701 gtttgaactc tgccggct
//
LOCUS       DATAQL010000872         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_701936, whole genome shotgun sequence.
ACCESSION   DATAQL010000872 DATAQL010000000
VERSION     DATAQL010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701936"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..960)
                     /gene="rph"
                     /locus_tag="VHS97_15125"
     CDS             complement(241..960)
                     /gene="rph"
                     /locus_tag="VHS97_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX3449585.1"
                     /translation="MTRSHHRKNDQLRPVSIERGFVRNSPGSVLYRSGETAVLVTASI
                     SDTVPPFLEGKSIGWLTAEYAMLPGSTPTRIRRGADGRATEIQRLIGRSLRAIVDRQA
                     LGPWTIHIDADVFNADGGTRTAAITAAFVAVADAIRGRFGAELCRSVLKDSVAAVSAG
                     IVAGEPVLDLDYREDSTAEVDFNVVRLGKGGLVEVQGTGEGGTFSREQLDALMGLAEQ
                     GISQLTELQRETLGGDWPLEK"
     gene            complement(1001..>1813)
                     /gene="argF"
                     /locus_tag="VHS97_15130"
     CDS             complement(1001..>1813)
                     /gene="argF"
                     /locus_tag="VHS97_15130"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX3449586.1"
                     /translation="LAGRTLGLLFEKPSLRTRVSFEAAIARLGGNAIFLHGKDVGLGV
                     RESLVDFARVLSQYVDALAARTFSHSTVDELARSATIPVINALSDASHPCQAMADLMT
                     IEELRGSVAGKKLVFVGDGNNVARSLAAASALLGAQFVLAAPPGYEFPADYQLSFKSR
                     FPKVPLVVESDPKIAVDRADVVYTDVWASMGQEHEAEARRSVFAPYQVNKDLLAHARP
                     DAIFLHCLPAHRGEEVTSDVLDGPRSQVIPQAANRLHFQMALLVWLLGDDKV"
BASE COUNT          334 a          579 c          572 g          328 t
ORIGIN      
        1 cctgaacgac ttgaatgtaa gcttccttac ggcgaaaccc ctgactgcgt tcgcagccgc
       61 tggactggtg ttgaccgcgg cttacatcgc cacttcggcg acgagcagcc gtgctcgtga
      121 ccctccatgc gacgccggga ctcgaaacac gtcagccgag tgtaaatatt tttctcagta
      181 tcgcccggcg caggttgaat tcccaacgac cggtgcatta gccccgcggc tggcgcgcgc
      241 tcacttctcc agcggccagt cgccgccgag cgtctcacgc tgcagctcag ttaattgact
      301 gatcccctgc tccgcgagac ccatgagggc atcaagctgc tcgcgcgaga atgttccccc
      361 ttcgccggtc ccctgcactt cgaccaggcc ccctttgccc aggcgcacga cgttgaagtc
      421 gacctccgcg gtggagtctt cgcgatagtc gagatcgaga accggttcgc ccgcgacgat
      481 gcccgcactg acggcggcga cactgtcctt caagactgag cggcacaatt cggcgccgaa
      541 cctgcctcgg atcgcgtcgg cgacggcgac gaacgctgcc gtgatcgcgg cggtgcgcgt
      601 gccgccgtcg gcattgaaca catcggcatc gatgtggatc gtccatggcc ccagcgcttg
      661 acggtcgacg atggcgcgca ggctgcggcc gatcagccgt tggatctcgg tggcccggcc
      721 gtcagcgccg cggcggatcc tcgtgggcgt gctcccaggc agcatggcat attcggcagt
      781 caaccagccg atcgacttcc cctcgagaaa cggaggcacg gtgtccgata tgctagcagt
      841 caccagtacc gcggtctcac ccgaccggta tagcacactg ccgggactat tgcgaacaaa
      901 cccgcgctcg atcgagacgg gccgaagctg gtcgtttttt cgatggtgtg atcgggtcat
      961 cggcaatctc gaacctttgt attggaaacg gcggctctgg ctaaaccttg tcatcgccca
     1021 ggagccatac caagagcgcc atttgaaaat ggagccggtt ggccgcctgc gggatgacct
     1081 ggctacgcgg gccgtcgagt acgtcggagg tcacttcttc gccacgatgc gcaggcaggc
     1141 agtgcagaaa gatcgcgtcg ggccgtgcat gggccagcag gtctttgttg acctggtagg
     1201 gtgcgaacac gctgcgccgc gcttcggcct cgtgctcttg gcccatactg gcccagacgt
     1261 cggtataaac gacgtcggcc cggtcgaccg cgatcttggg atcggattcg acaaccaatg
     1321 ggactttggg gaaccggctc ttgaaactga gctgataatc ggcaggaaac tcgtagcccg
     1381 gcggcgcggc cagcacgaac tgcgcgccca gtagagccga ggcggccgcc agcgaccgcg
     1441 ccacgttgtt gccatcgccc acaaagacca gcttcttgcc ggcgacggac ccgcgcagct
     1501 cttcgatcgt catgagatcg gccatggcct ggcagggatg agaggcgtcg gaaagcgcgt
     1561 tgatgaccgg gatcgtggcc gagcgagcca gctcgtcgac tgtggagtgc gaaaaggtgc
     1621 gggcggccag ggcgtcgaca tactgactca agacgcgggc gaagtcgacg agactttcgc
     1681 gtacgcctag ccctacgtcc ttcccgtgca gaaagatcgc gttgcccccc agacgagcga
     1741 ttgccgcctc gaaactgact cgggttcgga gtgaaggttt ctcgaagagg agcccgagcg
     1801 tccggcctgc gag
//
LOCUS       DATAQL010000873         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3909229, whole genome shotgun sequence.
ACCESSION   DATAQL010000873 DATAQL010000000
VERSION     DATAQL010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3909229"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1239)
                     /locus_tag="VHS97_15135"
     CDS             complement(70..1239)
                     /locus_tag="VHS97_15135"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX3449587.1"
                     /translation="MKIGVPRESYPGERRVALVPAVIPSLLKGGFEVLVEAGAGTEAA
                     FPDAEYTAKGARILPGRAEVFDAAEIIVQVLCHGSNDATGRDDLVLMRPDHLLVGFLR
                     PLGSIETIREVAERGVTSFAVELMPRTTRAQSMDALTSMATIAGYKSVVIAADRLPRL
                     FPMLTTAAGTITPARVLVIGAGVAGLQAIATGRRLGAVVSAYDLRPASKEQVQSLGGR
                     FVELPVEAVDAQDARGYARAQDDTFYERQRDLLGQVVAESDIVITAAVIPGKKSPVLV
                     TADMVGRMAPGSVIVDLAAERGGNCELTRSGELIVVKHGVTIVGWLNLASTVPYHASQ
                     MYARNVSAFLLHLVKDGKPRLDLTDEIIRETLLTRGGEVVNARVRDFFSLAALPN"
BASE COUNT          324 a          545 c          482 g          272 t
ORIGIN      
        1 tataaacgct gccagcatga acacgtacag gctggtaatc aggtcgagct gcatgcggtg
       61 tctccggggc tagttcggca aggcggcgag cgagaaaaag tcgcgtacgc gggcattgac
      121 gacctcgccc ccacgcgtta acagggtttc gcggatgatc tcatccgtga ggtccaatcg
      181 cggcttaccg tccttgacca gatggagcaa gaacgcgctg acgttccggg catacatctg
      241 actggcgtgg tacgggactg tgctggcgag gttgagccag ccgacgatcg tgacgccgtg
      301 cttgacgacg atcaactcac cggacctggt cagctcgcaa ttgccgccgc gctccgccgc
      361 gagatcgacg attaccgaac ccggcgccat ccgccccacc atatccgccg tcaccagcac
      421 gggcgacttc ttgccgggaa tcaccgcggc cgtgatcacg atgtcgctct cggcaacaac
      481 ctggcccagg aggtcgcgct ggcgctcgta gaatgtgtcg tcctgagcgc gggcatagcc
      541 gcgggcgtcc tgggcgtcaa ccgcctcgac gggaagctcc acaaagcggc cacccaggct
      601 ctggacctgt tccttggaag ccggacgaag atcgtacgcc gaaacgacgg cccccaaccg
      661 gcgcccggtc gcgatcgcct gcagcccggc cacgccggcg ccgatcacca aaacgcgggc
      721 gggagtgatc gttcccgcgg cagtcgtcaa catgggaaac aagcgaggca gccggtcggc
      781 cgcgatcaca actgacttgt aaccggcgat cgtcgccatc gacgtgagcg cgtccatact
      841 ttgagcgcgg gtcgttcgcg gcatgagctc gaccgcaaac gatgtgacgc cccgctccgc
      901 aacctcgcgg atggtctcga tcgatcccaa ggggcggaga aatccgacga gtaaatgatc
      961 cggccgcata aggaccaggt catccctgcc ggtggcgtcg ttcgacccat ggcagagtac
     1021 ctggacgatg atttccgcgg cgtcgaagac ttcggctcgc cccggcagaa tcctggcgcc
     1081 cttcgcggtg tattcggcat ccggaaatgc agcctctgta ccggcaccgg cctccaccag
     1141 aacctcgaag ccacctttca agagactcgg aatcaccgcg ggaacgagcg ccacacgccg
     1201 ctcgccggga taagactctc gcggcactcc gatcttcatc aatcgtcctc cgacccgtgg
     1261 agcgtaggct gcacgaccga ccgacagacc cgatttttct cgattgccct cgcgcacgcg
     1321 ctcgatttct cgcgcactgc cggaagcggc cagggcgcga tgtgcccgag cggcacgaaa
     1381 cgacccccac ggaacgcggg aggggacgaa acgacccgac tcccctccgg cgaccgactc
     1441 acttgctttg accctttcac aaagctcgat ccgttacaat agcatccgga gcttgagatt
     1501 ctacccccca atctccgaaa aagttcgtgc gtgaccacgc tgagcgattc gacggacata
     1561 tccagattga cgaggacttt gtcgccgtgc tcgacgtagc ggatcgcggc ttgcgatact
     1621 cgc
//
LOCUS       DATAQL010000874         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4823694, whole genome shotgun sequence.
ACCESSION   DATAQL010000874 DATAQL010000000
VERSION     DATAQL010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4823694"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VHS97_15140"
     CDS             <1..941
                     /locus_tag="VHS97_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX3449588.1"
                     /translation="ENPRTLDAAIAEAKRQGADLVLASDPDADRIGVAVPSGPEGEWT
                     TLDGNQIGVLLAAFVMKETEALGKLRPDHYVVTTLVSSPMARLLAQREGIRIEDDLLV
                     GFKWIASRIEEAGHASFLFAFEESHGYLKGNHARDKDAAVGAFLFAELAAAVKDRQQT
                     VLEYLDDLYIDVGHHGDRPINKTYPGREGLTKIRLLMEALRERPPRRIGGLALTDVYD
                     YGLHEIRALNSSEVIRPLPEPTGDLLIFHSEQRGTHFAARPSGTEPKIKFYLFARTPV
                     VGPDDLAAAKIETEKRLDQMVHDLEQYLEDALNSAS"
     gene            complement(968..>2020)
                     /locus_tag="VHS97_15145"
     CDS             complement(968..>2020)
                     /locus_tag="VHS97_15145"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX3449589.1"
                     /translation="RRDLPWRATRDPYRILVSEMMLVQTTVTAVIPYFERFLNQFPDA
                     GSLAAAAESDVLKVWEGLGYYRRARQLQAAAQTIVREHGGAIPDDPTLVRALPGVGRY
                     IAGAILSFAFDRPEPIVEANSQRVLARLLAISENLKTSSTLARLWQAAARLVPATGAG
                     IFNQALMELGALICTPREPACLVCPLLTLCEARRLGLQDRLPIVTRRPKPLAVTEACA
                     IVASAGRILIVQRARGGLWEQFWEFPTIHLAGVDPAGRSFGEPVDLAEGVRRLTGIVV
                     RPGPPVKSVLYSVTKHRVNLIAHLAQARAATPRPGPGLVDARWAEPAALGKYTFSSPS
                     RRLIDWINHEPDSPGA"
BASE COUNT          412 a          626 c          622 g          360 t
ORIGIN      
        1 ccgagaatcc ccgtactctg gacgcggcga tcgccgaggc caaaaggcaa ggggcggatc
       61 tcgttctggc cagtgatccc gacgccgatc gaatcggagt cgccgttccc agtggccccg
      121 agggcgaatg gaccaccctg gacggcaacc agatcggcgt attgctcgcg gcctttgtga
      181 tgaaggaaac cgaagccctc gggaagttgc ggcccgatca ttatgtggta acgaccttgg
      241 tctcgagccc gatggcacga ctgctggctc agcgtgaggg gattcgtatc gaggatgact
      301 tgctcgtggg attcaagtgg atcgccagcc gcatcgagga agcaggccac gccagttttc
      361 tgtttgcctt cgaggaatcg cacgggtact tgaaaggaaa tcacgcacgc gacaaagacg
      421 ccgccgtggg tgcgttcctc ttcgccgaac tggccgctgc cgtcaaggac cgccagcaga
      481 cagtcctcga atatctggac gacctctaca ttgatgtcgg ccatcacggc gatcggccca
      541 tcaacaagac gtatccaggg cgcgaaggtc tcaccaagat caggttgctt atggaagcct
      601 tgcgcgaacg ccctccccga aggatcggcg gccttgcgct tactgatgtt tatgactacg
      661 gtcttcatga aatcagagcc ttgaatagtt cggaggtgat tcgccccctc ccggagccca
      721 ccggcgattt gctcatcttt cattcggaac agcgcggtac tcatttcgcg gcgcggcctt
      781 cgggaaccga gcccaagatc aagttttacc tgttcgcgcg gaccccggtg gttggtcccg
      841 acgacctcgc cgctgccaag atcgagaccg agaaacgtct cgaccaaatg gttcatgacc
      901 tcgagcaata cctggaagac gccctcaatt ccgcatcgtg atcgaatgga attgggctca
      961 gtgaccccta tgcaccaggc gaatccggct catgattgat ccagtcaatg agccggcggc
     1021 ttggtgagct gaaggtatat ttgccgagcg cggccggctc ggcccagcga gcatcgacga
     1081 gacccgggcc gggacgggga gtcgccgccc gagcttgtgc gaggtgcgcg atcaaattaa
     1141 cgcggtgctt ggtgacgctg taaagaacgg acttgacggg cggacccggc cgaaccacga
     1201 tccccgtcag tcgtctgacc ccctccgcca ggtcgaccgg ctctccgaag gacctgcccg
     1261 ccggatccac accagctaag tgaatggtcg gaaactccca aaactgctcc catagccctc
     1321 cgcgcgcccg ctgaacgatc aagatcctgc ccgcgctggc gacgatcgca caggcttcgg
     1381 tgacggccaa aggcttgggt cgccgagtca caatcggcaa gcggtcctgg agcccgagtc
     1441 gccgggcctc gcaaagcgtt aggagaggac agaccagaca tgccggctcg cgaggcgtgc
     1501 aaatcagggc cccgagctcc atcagcgctt ggttgaagat tcccgcaccg gtggcgggca
     1561 ccagtcgcgc ggcggcttgc cacaggcgag ccaaggtcga ggacgttttg aggttctcgc
     1621 tgatcgcgag tagcctcgcc agcacgcgtt gactgttggc ctcgacaata ggctcgggtc
     1681 gatcgaacgc gaacgagaga atcgctccgg cgatatagcg gcccacgccc gggagagcgc
     1741 gcacgagagt gggatcatcg ggaatcgcgc cgccatgttc ccgcacgatc gtttgcgcgg
     1801 ccgcctgaag ctgcctggca cggcggtaat agcccagacc ctcccatacc ttgagcacgt
     1861 ccgactcggc agcagcagcc agtgagccgg catccggaaa ttgattgagg aatctttcaa
     1921 agtacggaat gaccgccgtc acagtggttt ggaccagcat catctccgat accaggatgc
     1981 ggtacgggtc gcgggtggcc cgccagggta agtcccgccg
//
LOCUS       DATAQL010000875         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2687969, whole genome shotgun sequence.
ACCESSION   DATAQL010000875 DATAQL010000000
VERSION     DATAQL010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687969"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1375
                     /locus_tag="VHS97_15150"
     CDS             179..1375
                     /locus_tag="VHS97_15150"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3449590.1"
                     /translation="MRLAAPVRCVCPGVLTCLVACVFAWSGTPAGAADAPPIARLLQP
                     FVDNQTLAGAVTLVASPERILDLEAVGWADIAAKKPMRADAVFWIASQSKPITAAALL
                     ILVDEGKVNVDNPVEKYLPEFKDLWVAAESDNEHILLKRPRHPILVREVLSHTSGMPF
                     ASPIEKPTLDLFPLALRVRSYAMLRLLFDPGSKSKYSNAGINTAGRIIEVVSGMPYES
                     FLDERLFKPLGMKDTTFWPAGEQLARVPKAYKPAKDGGLEEIPINQLQYPLDSRERQP
                     MPAGGLFSTANDMSIFYRMLASGGVFEGKRILSENTVRQMTSDQSGEAHSNYGFGIGT
                     NGQGFSHGGAYNTNSAYDGERKLITVFLVQHAGWGKDGKTIQPLFQKTSRKLFGARSA
                     ATAAAP"
     gene            1503..1661
                     /locus_tag="VHS97_15155"
     CDS             1503..1661
                     /locus_tag="VHS97_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449591.1"
                     /translation="MDAVPGDNPCPPKPFDPFTRDELLEYWRICDGVINGGGVTMRTP
                     SARPTRLA"
     gene            complement(1645..>2164)
                     /locus_tag="VHS97_15160"
     CDS             complement(1645..>2164)
                     /locus_tag="VHS97_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449592.1"
                     /translation="DPRKIHIARGAYDLIWVGSHFTHLDARLWWEFLEVFRDALSPGG
                     LFIFTTHGRIPYRNIVGGSWAYGTTGRRRTLLLHDYERTGFGYVRYANSDGYYGISLQ
                     SQAWVLNQVTKHGGLRVVHFAEQAWHRHQDCFACTREHSPQYRADSSTIPLWKYLKHL
                     LGEKRRDFKPVW"
BASE COUNT          424 a          637 c          665 g          438 t
ORIGIN      
        1 gcggtcaggc atcgtgggca actttccttc gagatctggg tgccgggcca cttgtctcgc
       61 gagtgctcac taattattgt ggcttgcgtg gtgaacgaag gcgctgtagc cgcacaccgg
      121 tctttggacg tggcaccttc ggagctcgaa cgttttgcct cgcagtcgag ggatttcaat
      181 gagattggca gccccagtcc gatgcgtttg tcccggcgtc ctgacctgcc tggtcgcatg
      241 cgtttttgcg tggtccggca cgcctgcagg cgcagccgac gcgccgccta tcgcgcgcct
      301 tctccagcca ttcgtcgata accagacact ggcaggcgcg gtgacgcttg tcgcctcgcc
      361 ggaaagaatc cttgacctcg aagcggtagg ttgggcggat attgccgcga agaagcccat
      421 gcgtgccgac gccgtatttt ggatcgcgtc gcagtcgaag cccatcaccg cggcggcgct
      481 cttgattctc gtcgacgagg ggaaggtgaa cgtggacaat ccggtggaga agtatctgcc
      541 ggagttcaag gatctatggg tcgccgcgga aagcgataat gagcacattc tgctgaaacg
      601 cccgcgtcac ccgattctgg tgcgcgaggt tctcagccac acaagcggga tgccttttgc
      661 ctcgcccatt gaaaagccga cgctcgatct ctttccgctc gcgttgcgcg ttcgcagcta
      721 tgcgatgctc cggttgcttt tcgatccagg gtcgaaatcg aaatactcga acgcgggcat
      781 caacaccgcg gggcggatca ttgaggtagt gagtggaatg ccgtatgagt cgttcctcga
      841 cgagcgactc ttcaagcctc tggggatgaa ggacacaacg ttctggcctg ccggcgaaca
      901 gctcgcgcga gtgccaaagg cttacaagcc cgcgaaagac ggcggcctcg aggagattcc
      961 gatcaatcaa ctccagtatc ccctcgactc tcgcgagcgg cagccgatgc cggccggcgg
     1021 cctcttctcg actgctaatg atatgtcgat cttctatcgc atgctcgcta gtggcggcgt
     1081 ctttgagggc aaacggattc tgtccgagaa cacggtgcgg cagatgacgt cggatcaatc
     1141 cggcgaagcc cactccaact acggctttgg cattgggact aacgggcaag gcttttccca
     1201 cggtggcgct tataacacaa actcagccta cgacggcgag cgtaagctca tcacggtttt
     1261 cctcgtccag cacgccggtt ggggcaagga cggcaagaca atccagccgt tattccaaaa
     1321 aacgtcgcgc aagctatttg gcgcaagaag cgccgccacc gccgctgccc catgatgaaa
     1381 gattgaaccc ggactccgtg gagttggcga tggaccatct tttgggaccg aactggctct
     1441 caaccgctcg gcgcaggcgg ctcttcatca cgtgagccag gcatcaagaa gaagcgaact
     1501 ttatggacgc cgttcccggg gacaacccat gcccgcccaa gccctttgac ccgtttacgc
     1561 gcgacgaatt gctcgaatac tggcgtatct gcgatggcgt gatcaacggc ggcggcgtca
     1621 ccatgcggac gccgtcggcc cggcctacca gactggcttg aagtctctcc gtttttctcc
     1681 gagaagatgc ttgaggtatt tccacagggg gatagtcgag gaatcggcgc ggtactgggg
     1741 ggaatgttcg cgtgtgcagg cgaaacagtc ctggtgacgg tgccaggcct gctcggcgaa
     1801 atggactacc cgcagcccgc cgtgcttggt cacctggttc aggacccacg cctggctctg
     1861 aagcgagata ccgtagtaac cgtcggagtt ggcgtagcgg acgtacccga atcctgtccg
     1921 ttcgtagtcg tggagcaata aggttctcct ccgcccggtg gtgccgtaag cccaggaacc
     1981 gccgacgatg tttcggtagg gaatccggcc gtgcgtcgtg aagataaaga gccccccggg
     2041 actcaaggca tcgcggaata cctcgaggaa ttcccaccac agccgggcat cgaggtgggt
     2101 gaagtgcgag cccacccaga tcaggtcgta ggctccgcgg gcgatgtgga tcttgcgggg
     2161 gtca
//
LOCUS       DATAQL010000876         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_90695, whole genome shotgun sequence.
ACCESSION   DATAQL010000876 DATAQL010000000
VERSION     DATAQL010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90695"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VHS97_15165"
     CDS             <1..763
                     /locus_tag="VHS97_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEX3449593.1"
                     /translation="AGRGSAGTGEVTATAFSVPEGTLDFWAERLKSLKIDIDGPFTRF
                     GERLISISDPDGMRVELVATARGDANKSWNGGPVPVDSAIRGLHHATLSEEGYERTAT
                     LLTVTLGFELLGQEGNTFRYALGDKAPGAIVDVECAPDRPPGRVAVGSVHHIAWRTPD
                     DPQQAVWRREIASLGYNVSPIMDRNYFHSIYFREPGGILFEIATDPPGFTVDEPLESL
                     GSKLCLPEVLEPHRALIVARLPSLRLPDARAGRST"
     gene            760..1389
                     /locus_tag="VHS97_15170"
     CDS             760..1389
                     /locus_tag="VHS97_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3449594.1"
                     /translation="MTTPNLTFLHRFVPSEPPISPTTLLLLHGTGGDENDLIPLGKAL
                     APGAALLSPRGKVLENGMPRFFRRLAMGVFDEEDLKVRTHELAAFITDSITSYDLDAH
                     KIIAVGYSNGANIASSLLLLHPGLLKGAVLFHPMVPFVPEVVPDLSNVPIFIGAARHD
                     EIVPPEETERLATLLENCRADVSVYWGPGGHGLSPAEVEAARRWLTRYL"
     gene            complement(1416..2183)
                     /locus_tag="VHS97_15175"
     CDS             complement(1416..2183)
                     /locus_tag="VHS97_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3449595.1"
                     /translation="MSQLGIVTYNIAKDWDLPTILSRLEKLGYEGVELRTSHAHKVEV
                     NLNKAERDLVRKRFQDSPVQLAGLGSAFEYHAADPAVVRKNVDGTKEYVRLAHDIGSP
                     GVKVRPNGVPRGADLDATLRQIGRALHEVGQDAADFGVAVRVEVHGSVTSELEKFSRM
                     LAYADHPNVYACWNSNPSDVKNGSIKPTFASVASKVHEVHLRDLTDETYPWRELFALL
                     KAQKFEGFTLAEIPESPDPERVLRYFRSLWLAYQPRA"
BASE COUNT          554 a          880 c          916 g          593 t
ORIGIN      
        1 tgccggcaga ggctcggccg ggaccggaga ggtaaccgcg accgcctttt cggtgcccga
       61 ggggacgctc gatttctggg ccgaacgtct gaaaagtctg aagattgaca tcgatggtcc
      121 gttcacgcgg ttcggcgaac ggttgatctc gatctcggat ccggatggca tgagagtcga
      181 actcgtcgcc acggctcgcg gcgacgcaaa taagtcatgg aatggagggc ccgtgcccgt
      241 tgactcagcc atccgcggcc tccaccacgc cacactctcc gaagaaggat acgaacgcac
      301 ggccacgctc ttgaccgtca cgctcggctt cgagctcttg ggccaggaag gcaacacctt
      361 tcgctatgcc cttggtgaca aggccccggg ggctattgtc gacgtggaat gtgcgcccga
      421 tcggccccca ggccgggttg cggtcgggag cgtgcaccac atcgcctggc gcactccaga
      481 tgatccacag caagcagtct ggcgtcggga aatcgcaagt ctaggctaca atgtctcgcc
      541 catcatggat cggaattact tccattcaat ctactttcgc gagccgggcg gcatcctttt
      601 cgagattgcg accgatccgc cgggtttcac ggtcgatgag ccgctcgaaa gtctgggttc
      661 caaactgtgc ttacctgaag tgctcgagcc ccatcgcgcc ttgatcgtgg cgcgcctgcc
      721 atcgctccga ctgcccgacg cccgggccgg gagatcgaca tgacaacccc gaacctgacc
      781 ttcctccatc gcttcgtccc gtccgagccg ccgatcagtc cgaccacgct gctcctcttg
      841 cacggtacgg gcggcgatga gaacgacctg attcctctgg gcaaagccct cgcacctggc
      901 gcagctttgc tcagcccacg agggaaggtc ctcgagaacg gcatgccacg attcttccgc
      961 cggcttgcca tgggtgtgtt cgacgaggaa gacttgaagg tccgcaccca tgagctcgcc
     1021 gcattcatca cggactcgat cacgtcgtac gatctcgacg cccacaagat catcgccgtc
     1081 ggatactcga atggcgccaa catcgcctcg agtctcctct tgctccatcc cggtttgctc
     1141 aagggtgccg ttctattcca tccgatggtg ccatttgttc ccgaagttgt ccccgatctt
     1201 tcgaacgtgc cgatctttat cggggccgca cggcacgacg agatcgtccc acccgaggag
     1261 accgaacggc tggcgacact gttggaaaac tgccgcgccg acgtctcggt ctactgggga
     1321 ccgggcggac acggcttgag ccccgccgag gtcgaggccg ctcggcgttg gcttacgcgg
     1381 tatttgtaaa ttggcctgcg accttgccct cgggatcacg cccgtggctg ataggccagc
     1441 cagagcgagc gaaaataccg cagcacgcgt tccgggtcgg ggctttcggg gatctcggcc
     1501 agcgtgaagc cctcgaattt ctgcgctttg agcaaagcga atagctcgcg ccaggggtag
     1561 gtctcatcgg tgaggtcgcg taggtgaact tcgtggacct tggacgcgac cgaggcgaat
     1621 gtcggcttga tcgagccgtt cttgacatcg gacgggtttg agttccagca ggcgtagacg
     1681 ttgggatggt cggcgtaggc gagcatgcgt gagaatttct cgagttcgga cgtgacgctg
     1741 ccgtgaactt ctacgcggac ggcgacacca aaatccgctg cgtcctggcc gacctcatgg
     1801 agcgcgcggc cgatctggcg cagcgttgcg tcgaggtcgg ctcctcgggg aacgccgttg
     1861 ggccggacct tcacgccagg cgacccgatg tcgtgggcca gtcggacata ctccttggtc
     1921 ccgtcgacgt ttttacgaac cacggctgga tcggccgcgt ggtactcgaa ggcgcttcct
     1981 agcccggcca gttgcacggg cgagtcttga aatcgcttgc ggaccaggtc gcgctcggcc
     2041 ttgttcagat tgacttccac cttgtgggca tgcgacgtcc gcagctcgac gccttcgtat
     2101 cccaactttt cgagtcgcga cagtatcgtg gggaggtccc aatccttggc gatgttgtac
     2161 gtgacgattc cgagctgaga catcgcgata agcccccgtg aagacgagtt gtcggtactg
     2221 gagtgataat ggccgggtcc gctttggtga cagccgtgtc gtcggcctat cataatccgc
     2281 gggctttcgg gcgcccatct ccaagaaaga cggtcggaaa cgccggcgac ctgaacctcg
     2341 ttttctggtg gcaaccaatt acggatttta cggttatggt atgtcagtcg ctattgtgca
     2401 tggtgtcgat cctgtacccc agggctggca agggctggac gtgcgatctc gggcggctta
     2461 atgttgaggg atcaagcgta atgaccgggt agctcagtcg gtagagcaac ggacttcaga
     2521 ggagtgaacg gagcctcgcg cgggaagaaa cggaaccgcg atgagtgaat gccgcaatat
     2581 gctgggacgc cctgagagcc gagcgcacca caacgtcgcc ggtaacggca agcgtgatgg
     2641 tttgaaaagc gtcggattgg gtaatcagca ggcaagagac cggccgttgg ccggcctcag
     2701 cctcagagac tagacgcggc acgcctgcga tggcgatggg atagtccaga ctttcaactc
     2761 gggaccaagg ctagtcgcga gcgattgcgt cattgcccaa gtcacggcgg tcggtgaaag
     2821 ccgatgaggt aagttaatcc gttggtcgag ggttcgagtc cctccccggt cactaagcag
     2881 cgaagcgctg gaaacaggcg tgaaaccctc agaaatgagg gtttttccgt gcgctctcgt
     2941 ccc
//
LOCUS       DATAQL010000877         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5435064, whole genome shotgun sequence.
ACCESSION   DATAQL010000877 DATAQL010000000
VERSION     DATAQL010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5435064"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /gene="thiO"
                     /locus_tag="VHS97_15180"
     CDS             <1..1109
                     /gene="thiO"
                     /locus_tag="VHS97_15180"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX3449596.1"
                     /translation="GVIGLSVALALAREGIRPAVLDRRELGREASWAGAGLIPPQADN
                     PARQPLVALGSWSARLYPAWSESLHVETGIDIGYRRTGGVNVAWTDLENQELLATSGR
                     WRALGIAYERLPKGDYTRVEPALNPSLESVYFLPDRAQVRNPRLLRALMAAIARKGGT
                     LMPECGVESFQVHAGRVTAVKTGAGELPCGTVVVAAGPWSGQLLQSIGVNAPTPPFKG
                     QIVLLRGNRPLLRRIVEHGNNYLVPRDDGRILVGATEENVGFDSIPTARDTRALVDWA
                     VRICPVLKEAEIQTTWAGLRPGSFDSKPYIGRAPGFDNVIVASGHKRAGLQLAPATAE
                     LVADLVLDRSPRLDIGPFRIDREPEPAEDVTFRS"
     gene            complement(1205..1873)
                     /locus_tag="VHS97_15185"
     CDS             complement(1205..1873)
                     /locus_tag="VHS97_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3449597.1"
                     /translation="MATVATAESRRPPPTQAGVILHDVTWNQYEAMLRIVGERPIRVT
                     YDTGTMELFMPSLGHESDAYLLGRMVDMLTEELEISMMGGGTTTHKRQDLDKGAEPDQ
                     CYWFGENAQRIRGKRTLDLDIDPAPDLVIEVDVTRGSLDRLRIFAALGVLEVWRSDGE
                     TLQFLHLLTDGSYQPGPISRSFPGLPVFAVAQFLEQGRASDTTAWVRSFRAFVREQVV
                     PQNG"
     gene            complement(1996..2712)
                     /locus_tag="VHS97_15190"
     CDS             complement(1996..2712)
                     /locus_tag="VHS97_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX3449598.1"
                     /translation="MASLTVENYVKTIALIAARNPEGSLVGTGELAQALNVSPGTVTG
                     MLKTLSEAHLATYTPYEGARLTESGQRLAMKVIRRHRLLERFLAETLTMSWDEVHEEA
                     EHMEHAASERLIDRIDAYLGYPEVDPHGDPIPRADGSLAQTEGVPLARCQPGQHFRVV
                     RVMDQDPAFLRYLSECGLELHATGELLVNRPEAGALVCLLGGRNVALGLAAAGKLMVT
                     LTNPANSLSGTDLTSGKQRG"
     gene            complement(2876..2946)
                     /locus_tag="VHS97_15195"
     tRNA            complement(2876..2946)
                     /locus_tag="VHS97_15195"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2912..2914),aa:Cys,seq:gca)
BASE COUNT          584 a          936 c          919 g          570 t
ORIGIN      
        1 gcggtgtgat cggcctgtcg gtcgctcttg ccctggctcg cgagggaatt cggcccgcgg
       61 ttctcgatcg ccgcgaactg gggcgcgaag cgtcatgggc gggtgctggt ctgattccgc
      121 cccaggccga caatcccgcg cgacagccgt tggttgcgct cggctcgtgg agcgcccggc
      181 tctacccagc ctggtcggaa tcgcttcatg tcgagacggg tatcgatatc ggctaccgcc
      241 gcaccggtgg agttaacgtg gcctggaccg atcttgaaaa tcaagaattg ctggcgacct
      301 ccggccgctg gcgagcgctc gggatcgctt acgaacgtct gccaaagggc gactacacgc
      361 gtgtcgagcc ggcgttgaat ccctcgctcg aatctgtcta tttcttgccg gatcgggccc
      421 aagtgcgcaa tccgcggcta ttgcgagccc tcatggcggc gatagcccga aagggcggca
      481 cactgatgcc ggagtgcggc gtcgagagtt ttcaggtcca tgccggccgg gtcacggccg
      541 tcaaaaccgg cgccggcgag ctgccgtgcg gaaccgtggt cgtggcggcc ggaccttggt
      601 ccggtcagct attgcaatca atcggagtga acgcccccac gcctccgttc aagggacaga
      661 tcgtcctgtt aagaggtaac cggcccttac tccggaggat cgtcgagcac ggcaacaatt
      721 acctggtacc acgcgacgac ggccggatcc tggtcggcgc caccgaggaa aacgtgggat
      781 tcgactcgat accgaccgcg cgcgacacac gcgcgctcgt cgattgggca gtccggattt
      841 gtcccgtgtt gaaggaggcc gagatccaaa cgacctgggc cggtctgcgg cccggcagtt
      901 ttgatagcaa gccttatatc ggccgggcac ccggctttga caacgtgatc gtcgcctcag
      961 gacataaacg cgccggcttg cagctcgctc cggccacagc ggagcttgtc gccgacctcg
     1021 tgctcgaccg ctcgccacga cttgacatcg gcccgttccg aatcgaccgt gaaccggaac
     1081 cggcggagga cgtcacgttc cggtcatgag cttccgcgaa ctcataatcc cgaaacgatc
     1141 atgcaggtgt ctgcagccga gctctgaccc agtcgaactg tgacagctcc tgaacctgtg
     1201 gaggtcaacc attctgaggt acgacctgct cgcggacgaa cgcccggaat gagcgaaccc
     1261 aggccgtcgt gtctgaggct cgtccctgct ccaggaattg agcgactgca aacactggca
     1321 aacccgggaa acttctgctg atcgggccag gctgataaga accgtccgtt agaaggtgaa
     1381 gaaactggag agtttcacca tcagaacgcc atacttcgag cacacccaat gctgcgaaaa
     1441 tcctgagccg atccaatgag ccgcgtgtga cgtccacctc aatcaccaaa tcaggggcgg
     1501 ggtcgatatc caagtcgaga gtgcgctttc cccggatgcg ctgagcgttt tctccaaacc
     1561 aatagcactg atcgggttcg gctcctttgt cgagatcctg tcgcttatgg gtcgtagtgc
     1621 cgcctcccat catcgaaatc tcgagttctt ccgtgagcat atcgaccata cggcccagga
     1681 ggtacgcgtc gctctcatga cccagtgacg gcatgaagag ctccattgtt cccgtatcgt
     1741 aggtgacgcg tatgggccgt tcacccacaa tccggagcat cgcctcatat tggttccacg
     1801 taacgtcgtg cagaatgacc ccggcttggg tcggcggagg acgcctggat tccgctgtag
     1861 ccactgtggc catgaacgag cctttcggaa tggcttgaac gcaggaatat cacccgatcc
     1921 aaaaattgta ccatgtcgag cggactatac tgcaaggcat ggagaccgct ggcgcactgg
     1981 caaaatggac gggtcttagc cgcgttgttt ccctgacgta agatctgttc cggataatga
     2041 attggccgga ttcgtgagcg taaccatcaa cttgccggcc gccgccaatc ccagtgcgac
     2101 gttccgcccg ccgagcaagc agaccaaagc gcccgcttcg ggccggttga ccagcagctc
     2161 gcccgtcgca tgcagctcga ggccgcattc actcagataa cgcaggaagg cagggtcttg
     2221 gtccatcacc cggacgaccc ggaaatgctg gcctggctgg caacgggcca gtggaacccc
     2281 ctcggtctgg gctagcgatc cgtcagcgcg ggggatgggg tcgccatgag ggtcaacttc
     2341 gggataaccc agataggcat cgatccgatc gatcagccgt tcggaggcgg catgctccat
     2401 gtgctcggcc tcctcgtgga cctcgtccca agacatcgtg agcgtctcgg ccaggaaccg
     2461 ctcgagcagg cgatggcggc ggatgacctt catcgccagc cgctgacccg actcggtcag
     2521 gcgagccccc tcatagggcg tatacgtggc cagatgggct tcggagagtg tcttgagcat
     2581 gccggtgacc gtgccgggcg aaacgttgag cgcttgcgcc agctcaccgg taccaacgag
     2641 gctgccttcg ggattacggg ccgcgatcaa ggcaattgtc ttgacgtagt tttcgacggt
     2701 cagactggcc acagcgtgct tcggcctccc aactcggaac gatcgtcgcc aaatcaaagc
     2761 cgcgacgatc cggttcacgg ccattctacg gccagtcatc ctacgcccgg ccgtcacgtc
     2821 ggggaagccg gatcggtcct ggctccaaca gccggccgtg gaaatggtga caaaaagacg
     2881 ccggtgggag tcgaacccac ttcaaccggg ttgcagccgg tcgcctggcc gtctggctcc
     2941 agcgtcgtgg tggcgaatgt cctcgccagg aattgaacct ggtcctcgac cttcgcaggg
     3001 tcgtatgcg
//
LOCUS       DATAQL010000878         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1007843, whole genome shotgun sequence.
ACCESSION   DATAQL010000878 DATAQL010000000
VERSION     DATAQL010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007843"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1957
                     /locus_tag="VHS97_15200"
     CDS             224..1957
                     /locus_tag="VHS97_15200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449599.1"
                     /translation="MSDPHDTTEDHIPRDPATASAADRDSFVPSQRVVDQRGTRLGTE
                     TRWLLQERQRAASLVLVVGFGLFFVRSLILHWDKLESLAVLFHGIMLGLLLLSLAALS
                     NRWKRTVRQLRSFEVVLFGLIIVFFMAAQYVLMLRGVRENDQVRLLAAVKSSVLWMLA
                     MIFTYAIFIPNHWVRAAKLIVPMALAPMAVPWILGLFHPEFYEVAIRAANLEKISEDG
                     LFLLLGAFTAIFGTHTINTLRIEAYEAKLLNQYRLGRKLGGGGMGEVYEAEHQLLKRP
                     CAIKLIRHELAGNQRVFARFEREVRATALLSHWNTVEIFDYGRNDDGAFYYVMEYLPG
                     LSLADLVERHGPMPPARVIYLLRQACDALTEAHDSGLIHRDIKPANLMAAYRGGHYDV
                     TKLLDFGLVKTLAENESIHLSQEGTVAGSPLYMAPEQVMHNQPPDRPTDIYGLGAVAY
                     FVLTGRPPFLGQTAMEVMVAHARDPVPPPSQLRADIPADLEAVVLRCLAKNPQDRYPD
                     TTSLARALDACADAPNWSPEHAAHWWQTTASSSSNHYNNRTVRLPPDAITQESPMPTQ
                     EMESAEVPRIL"
     gene            1985..3121
                     /locus_tag="VHS97_15205"
     CDS             1985..3121
                     /locus_tag="VHS97_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449600.1"
                     /translation="MNLTTDRPDPDINRREATSEAIVATIHLAFAFDIGDEIDLDRAR
                     LILQGEPGQLPRRRRTPESIGYRPAPIRVELEPAGIELPGKWSFIKPPRGELTFFDFG
                     AISLAVQFPVNTTAGDLLALAGRLAETAPLTEAARRLLSPWLERIKPSVYDFAISDLS
                     EEYIVFQLWERRSDWLQERAAWIAGLVRLESEELSPDEVREATRLSLAYTPNDLVVLD
                     WAAGVVADSECADTLQVIEFANVQLLEFRHIDDRLDDRLEAAYRLIRPEKRRRYIPSR
                     WRLHGAAVRQIRELEIEATSLFERVDNALKLIGDNYLSRIFEVAGARFHLPGWQQSIR
                     RKLETVGDVYDLLVQQAGGQRMEALEITVVLLIALEIVLALLRH"
     gene            complement(3494..3706)
                     /locus_tag="VHS97_15210"
     CDS             complement(3494..3706)
                     /locus_tag="VHS97_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX3449601.1"
                     /translation="MAANYTAIIQQHSEWWIGWVEEVPGVNSQGHTREELLENLHDAL
                     EEALEMNRAEARAAASGAFEEVSISA"
BASE COUNT          739 a         1237 c         1120 g          755 t
ORIGIN      
        1 gccacgatgg atccatgatt ccgttgcggg cagatgacga gttatccggc gaagacgtcc
       61 ttcccggctt ccgctgcaag gtcgacgacc tctttccgaa acaaccgcac acggaggttc
      121 acagacccgc acactctgac cctgtctcgt cgccgcccag ttcgtgaagc cctttcgctc
      181 gcatgctgac cctttcccat cggccgattc cggtacaggt catatgtccg atcctcatga
      241 cacaaccgag gaccacatac cacgagatcc tgccacggcg agcgccgccg atcgcgatag
      301 cttcgtcccg agccagcggg tggtggacca gcgtggaact cgtttaggca ccgagacgcg
      361 ctggctgcta caggagcggc agagggccgc gtcgctggtc ttggtcgtcg gcttcggcct
      421 gtttttcgtg cggtcgttga ttctgcactg ggacaaactc gaatcactgg cggtcctgtt
      481 tcacgggatc atgctgggct tgttgctctt gagtctggcc gccctgtcta accgatggaa
      541 gcggacggtg cgtcaactcc ggtcgttcga agtcgtgctg ttcggtctga tcatcgtctt
      601 cttcatggca gcccaatatg tattgatgct gcgcggagtt cgcgagaacg accaggttcg
      661 gctgctggcc gcggtcaaga gcagtgtgct ctggatgctg gcgatgatct tcacctatgc
      721 gatcttcata cccaatcatt gggtgcgcgc agccaagctg atcgtgccga tggcgctggc
      781 acccatggcc gttccgtgga tcctgggatt gtttcatccc gagttttacg aggtcgcaat
      841 ccgggccgcg aatctggaaa aaatcagtga agacgggctg ttcctgctct tgggcgcatt
      901 cacggccatc tttggcacgc acaccatcaa caccttgcgc atcgaggcgt acgaggcgaa
      961 attgctgaac caataccgcc tcggacgaaa gctggggggc ggcggtatgg gcgaggtcta
     1021 cgaggccgag catcagttgc tcaagcgtcc gtgcgcgatc aagctgatcc gccacgagct
     1081 ggcgggcaat cagcgcgttt tcgctcgctt cgagcgagaa gtccgcgcga ccgcgctctt
     1141 gtcgcactgg aacacggttg agatcttcga ctacggtcgc aacgacgacg gcgccttcta
     1201 ttacgtgatg gaataccttc ccgggctcag cttggccgac ctggtcgaac ggcatggccc
     1261 gatgccgccg gcgcgggtga tctacctact ccggcaggcg tgcgatgcac ttaccgaggc
     1321 tcacgattcg ggcttgatcc accgcgacat caaaccggcc aatttgatgg cggcctatcg
     1381 cggcggccat tatgacgtga ccaaactgct cgatttcggc ctggtcaaga ctctcgccga
     1441 aaacgaatcg attcatctct cccaggaagg gaccgtcgcc gggtccccct tgtatatggc
     1501 ccccgagcaa gtcatgcaca accagccccc cgaccgaccg accgacatct acgggctggg
     1561 agcggtcgcg tacttcgttt tgacaggccg gccgccgttc ctcggtcaaa ccgcgatgga
     1621 ggtcatggtc gctcatgccc gagaccccgt tccgccgccg tcgcagcttc gcgccgatat
     1681 tcccgcagac ctggaagccg tcgtgcttcg ctgcctcgcc aaaaatcccc aagaccgcta
     1741 tccggacacc accagcctgg cccgagctct cgatgcctgc gccgatgcgc cgaactggtc
     1801 gcccgagcac gccgcccact ggtggcaaac caccgcgagt tccagctcaa accactacaa
     1861 caatcgaact gtcagactcc cccccgacgc gatcacacag gaaagcccga tgccaacaca
     1921 ggagatggag tctgccgagg ttccgcggat cttgtaacgc gaatcgcctc gaacaagcca
     1981 cgccatgaat ctgaccaccg accggcccga ccctgacatc aatcgcagag aagcgaccag
     2041 cgaggccatt gtcgccacga ttcatctcgc gtttgccttc gatatcggcg acgagatcga
     2101 ccttgatcgt gcccgcctga tcctgcaggg cgagcccggc caattgccgc gacgccggcg
     2161 cacgcccgaa tcgatcggct atcggcccgc gccaatccgg gttgagctcg agccggccgg
     2221 tatcgagctg ccgggaaagt ggtcgttcat caagccgccc cggggcgagc tcacattctt
     2281 tgacttcggc gcgatctcgc tcgccgttca gttcccggta aacacgaccg caggcgacct
     2341 gcttgcgctg gccggccgtc tggcggagac agccccgttg accgaagccg cccggcggct
     2401 gctctccccc tggctcgagc gcatcaaacc ttcggtctac gatttcgcca tcagcgactt
     2461 gagcgaggag tatattgtct ttcagctttg ggagcgccgc agcgactggc tccaggaacg
     2521 cgccgcctgg atcgccgggc tcgtacggct cgagtcagaa gagctcagcc cggatgaggt
     2581 gcgcgaggcc acacgcctct cgctggcata cacgcccaac gacctcgtcg tcctcgactg
     2641 ggcggctggc gtcgtcgccg actccgagtg cgcagacacg ttgcaagtca tcgaattcgc
     2701 caacgttcag ctcctggaat tccgccacat tgacgaccgg cttgacgacc ggcttgaggc
     2761 cgcctaccgc ctgatccgtc ccgagaaacg ccgccgctac atcccctcgc gctggcgact
     2821 gcacggcgcc gccgtccggc agatccgcga gctcgagatc gaggcaacga gcctcttcga
     2881 gcgggtcgac aacgccctga aactgatcgg cgacaattac ctttcccgca tcttcgaggt
     2941 cgccggagcg cggttccacc tccccggctg gcaacagagc atccgccgca agctggagac
     3001 agtgggcgac gtttatgacc tcctcgtcca gcaagccggc ggccagcgga tggaagcact
     3061 cgagatcacc gtcgttttac tgatcgctct tgaaatcgtg ctggccttgc tgcggcactg
     3121 agccgtcacg gcggctttgc aaaatcttcg gacgcgcgac gtgagtcagg ctttccagct
     3181 tttcagcctg acatcggcct gaaaggctga cctactctcg cttggcagct gcccgagaca
     3241 gtcttcgaat catgaaaatt cgtgaccatt cagccgagtg aggaagggat cgaatgagga
     3301 acgcaggaag gcaggagcag gcagttttgg acagttcctg gtttcctggt ttcctcattg
     3361 gtcgcgtatt ggcggttacg gaattgcgct gaacgattgc gaaaattcat ttaggcgccg
     3421 cgaaagaata agttgagcaa gcgggcacgg cgggcttaag tggcacgggc atcttgcccg
     3481 tgggcgtgcc tcttcacgcc gaaatgctaa cttcctcgaa agcgccactc gcggccgctc
     3541 gagcctcggc gcgattcatc tccagtgcct cttcaagggc gtcatgcaag ttttccagca
     3601 gctcctcgcg ggtatggccc tgcgagttaa cgccggggac ttcctcgacc caaccaatcc
     3661 accattcgct atgctgttgg atgattgcgg tgtaattcgc tgccatgggg gcctccgccg
     3721 gtgcgattgg ggcacaatcc cgaacaaaga gtataccgat tcggcgcacc gggcgacaca
     3781 tggacaacct ttaagagcat ggcagcctgc gtagaaaagg ggtcaaaaaa gggtcaagaa
     3841 aaggggtcat t
//
LOCUS       DATAQL010000879         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2532697, whole genome shotgun sequence.
ACCESSION   DATAQL010000879 DATAQL010000000
VERSION     DATAQL010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532697"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VHS97_15215"
     CDS             <1..624
                     /locus_tag="VHS97_15215"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX3449602.1"
                     /translation="YEEASGEAAFYGPKADFIVRDCIGRQWQLGTVQLDYVLPERFEL
                     EYIGPDNQKHRPVMIHRAPLGSMERFMGILIEHFAGAFPLWLAPEQVRVLPISDKVAD
                     YSRQVLARLLEKGFRATLDLRAEKIGAKIRDAQLEKIPAMLVVGAKEAENQAVSYRDR
                     VDGDQGAMALEEALARLEAERASRRSRQTAPPPAALPVEEESEDHTY"
     gene            659..1654
                     /locus_tag="VHS97_15220"
     CDS             659..1654
                     /locus_tag="VHS97_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX3449603.1"
                     /translation="MSVDTTEIPAIEIGLDDIRRAAERLAPWAHRTPVFTCKALDGRC
                     GNSVFLKCENFQKIGAFKFRGAMNAVLQLSDAERRCGVITHSSGNHAQALALAGKLLD
                     VPVTVVMPRTAPAVKREATLGYGARIVACEPTFESRETTVAAEIEHHGFTLVHPFNDW
                     NVIAGQGTAAYELLEQAGTLDIVLCPVGGGGLLAGTALAVKGLAPATKVIAAEPAAAD
                     DALRSFESGSIQRCHDPKTIADGLRTTSIGERNFAVIARKVDRIISVSELAILAAMRF
                     LWERTKIVVEPSGAVAAAPLLEGTVAGEGLRVGVILSGGNVDLESFFHSLATKSV"
     gene            1683..3128
                     /gene="dcuC"
                     /locus_tag="VHS97_15225"
     CDS             1683..3128
                     /gene="dcuC"
                     /locus_tag="VHS97_15225"
                     /inference="COORDINATES: protein motif:HMM:NF037994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HEX3449604.1"
                     /translation="MNRPGVTGGFAISTRGVLGFFSALVFVAALVTGLGLPGLASPAS
                     RSWLGSAIASLDLVMVAALVSRGVDARMLLLLGALPLFVLARQLPEMVVKIGSEMANT
                     GTVIPICSAMGFAFVLRLTTCDQHLVRLLTEPIRKTRALLIPGGILAGYSINTTIVSQ
                     SGTAAVLGPVLIPLLRAGGLGPISAGAILLLGSSMGGELFNPGAVEMRKLSELTGFSS
                     AQVVARSARLNLLACATALGAFWVLVRRREENQASTTESPVEDDETEIGQTEPISLLK
                     AVVPLLPILLLTLDAVFGPYAFARPAEAPAKILAVMLIGIVAAGLASLPVSGQVTSAF
                     FEGAGYAYTHVISLIVTASTFAEGIRLSGLIDIVTKALVAWPGAAMIVAMAAPWSLAF
                     VAGTGIAPAIAIMEFFVPVAGTLGIDPVRLGTLSAMGAHFGRTMSPVAAVVIMSARLS
                     DTSAWDLIRLVAGPLLAGGVVLLAAVMLDLL"
     gene            3187..3684
                     /locus_tag="VHS97_15230"
     CDS             3187..3684
                     /locus_tag="VHS97_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX3449605.1"
                     /translation="MADSHSFDVVSEINHAEMHNAIVQAQHELAVRFDFKGTRASIEF
                     NKKENQLTLTADHKGQLDTVLQMVKEKMARRGVAVNALKRGKIEDATHDSVREILTLH
                     TGIESEDARKLVKEIKQLKIKVQAQIMDEKVRVTGKKLDELQAVIAHLKAHGPEYPLQ
                     FVNFT"
     gene            3852..5033
                     /locus_tag="VHS97_15235"
     CDS             3852..5033
                     /locus_tag="VHS97_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449606.1"
                     /translation="MGFWNGRVTFTRYQVSGEKPVPFGEPVIDQARAHLIGQHGAPDP
                     ADGVSIGWGGGEHLLDVELDGGKNIVDDALHLAIRIDTNKVPGTLLRAYTQIEIDARA
                     QLNPSGIATKIQRQEAKEAAKLRADAEAADGRFRRRNHFPVLWDGRNNILYAGSTSTT
                     VLDRLQSLFRETFDRTLEPITAGIMAKNLELNSPEGPGRRPSNLRERRFGIVGGTENS
                     GISVAWSEDDPDSLDYLGNEFMVWLWHALQNDGDSVKLADESHVTVMLAKTLTLDCPR
                     GEMGRDHLTDDEPIRLPEAFRALQAGKLPRKAGMVLVRHGADYELTLQAETLAVTGGA
                     LPKPEDRRLSVHDARIARLEGVRHLAETIDLLFQAYLDRRTSSNWSDELSRIRRWLQA
                     A"
BASE COUNT          995 a         1512 c         1692 g          998 t
ORIGIN      
        1 tacgaagagg cgagcggaga ggcagcgttt tacggaccca aggccgattt catcgtgcgc
       61 gactgtattg gccgccagtg gcagctcggt acggtccagc tcgattatgt gctgccggag
      121 cggttcgagc tcgagtacat cgggccggac aatcaaaagc atcggccggt gatgattcat
      181 cgcgcgccct tgggaagcat ggagcggttt atggggatct tgatcgagca tttcgcgggt
      241 gcgtttccgc tctggctagc tcccgagcag gtgcgagtct tgccgatctc cgataaagtt
      301 gccgactact cgaggcaggt gctggcgagg ctgctcgaga aaggcttccg ggcgactctc
      361 gacctgcggg ccgagaaaat cggggccaag attcgcgatg cccagctcga aaagatcccg
      421 gccatgctgg tggtgggagc gaaggaggcc gaaaatcagg cagtctcgta tcgcgaccgg
      481 gtcgacggtg atcaaggggc gatggcgctg gaagaagcgt tggcgcggct ggaggccgaa
      541 cgcgccagcc gccggagccg ccagaccgct cctccgccgg cggcgctgcc ggtcgaggaa
      601 gagagcgaag atcacacgta ctgaaaaaaa ccgacgtgac actagccgag gcgtattcat
      661 gagcgtcgat accacggaaa tcccagcaat tgaaatcggt ctggacgaca tccggcgagc
      721 ggccgagcgg ctggctccgt gggcccaccg cacacccgta ttcacatgca aagcgcttga
      781 tgggcgatgc ggcaactcgg tgttcctcaa atgcgagaac tttcaaaaga ttggcgcatt
      841 caaattccgc ggagcaatga atgccgtgct ccagctcagc gatgccgagc gccggtgtgg
      901 ggtgatcacg cattcctccg gcaatcatgc ccaggccctg gcgctcgctg gcaagctgct
      961 ggatgttcct gtcacggtag tcatgccgcg gacggctccg gccgtcaagc gagaggcgac
     1021 tctcggatac ggagcgcgaa tcgttgcttg cgagccgacg ttcgaatcgc gcgagacgac
     1081 ggtggcggcc gaaatcgagc atcacgggtt caccctggtg catccgttca acgactggaa
     1141 cgtgatcgcc ggccaaggga cggcagctta tgagctgctt gaacaggcag ggacgctcga
     1201 catcgtgctc tgtccggtcg gtggcggcgg tttgctggcg ggaacggccc ttgcggtcaa
     1261 gggattggca cccgcaacca aggttatcgc ggccgagccg gccgcggccg acgacgccct
     1321 gcgatcgttt gaaagcggct cgatccaacg ttgccacgat cccaagacga tcgccgatgg
     1381 cctgcgcaca acgtcgatcg gcgagcggaa cttcgccgtg atcgcccgta aggtcgaccg
     1441 gatcataagc gtcagcgagt tagcgatctt ggccgctatg cgattcctct gggagcggac
     1501 gaaaatcgtg gtcgagccct cgggcgctgt cgccgccgcg ccattactgg aggggacggt
     1561 cgcgggcgag ggtctgcggg tcggcgtcat cttgagcggc ggcaacgttg acctcgagtc
     1621 attcttccac tccctggcaa ccaaatctgt gtgaagcctg cccacactcg tcgagtgatt
     1681 ccatgaatcg tccgggggta actggcggat ttgcgatctc caccagaggg gtgcttggct
     1741 ttttcagtgc gctcgtattt gtggctgcgc tcgtcacggg gctcgggttg ccgggcttag
     1801 cttcgccggc ctcgaggtcg tggcttggga gcgcgatagc gagcctggat ctcgtgatgg
     1861 tggccgcgct cgtgtcgcga ggcgtcgacg cgcgcatgct gctcttgctc ggcgccttgc
     1921 cgctctttgt gctggcgcgc cagctcccgg agatggttgt caagatcggc agcgaaatgg
     1981 ccaacacagg gaccgtgatt ccgatttgtt cggcgatggg attcgcgttt gtgctgcggc
     2041 tgacgacctg cgaccagcac ctcgtgcgtc tcttgacaga gcccatccgc aagacgcgcg
     2101 cactcttgat tcccggcggc attctcgcgg gctattcgat caacaccaca atcgtgagtc
     2161 agtcggggac ggcggccgtg ctgggacctg tcttgatccc gttgctccgc gccggcgggc
     2221 tgggaccgat ttcggccggg gcgatcctgc tccttggctc gtcgatggga ggcgaactct
     2281 tcaaccccgg cgcggtcgag atgcggaaac tatccgagct gaccgggttc tcgagtgcgc
     2341 aggtggtcgc ccgatctgcg cggctcaacc ttctggcgtg tgccactgct ctgggtgcgt
     2401 tctgggtcct ggtcaggcgt cgtgaggaaa atcaggcctc aaccacggag agtccagtag
     2461 aagacgacga aaccgagatt ggccaaactg aaccgatcag cctcttgaag gcggtggttc
     2521 cgcttttgcc gatcttgctg ctgacgctag acgcggtctt tggtccgtat gcgttcgcgc
     2581 gcccggctga ggcgccggcc aagattctgg ccgtcatgtt gatcggcatt gtggcggcgg
     2641 gtcttgccag tctgccggtg tctggacagg tcacgtcggc ctttttcgag ggggccggct
     2701 atgcgtacac ccatgtcatt tcactgattg tcacggcctc gacttttgcc gaagggatcc
     2761 ggttgagcgg gctgattgac attgtgacca aggcgctcgt ggcgtggccc ggtgccgcaa
     2821 tgatcgtagc gatggcggct ccgtggagcc tggcattcgt ggcgggcacc gggattgcgc
     2881 cggcgatcgc gatcatggag ttttttgtgc cggtcgccgg cacgctgggc atcgatccgg
     2941 ttcggctggg aaccttgtcg gcgatgggcg cgcatttcgg tcgcaccatg agtccggtgg
     3001 cggccgtggt gatcatgtcg gcacggctgt cagacacgag cgcctgggat ctgatccgcc
     3061 tggtcgcggg gccattactg gccggaggag tcgtcttgct agcagcagtc atgctggatc
     3121 tgctctagga acgacgacct gggaatctgc aatctttcga tcgcctttcg ttggagtatc
     3181 taacccatgg ccgattcaca ttcgttcgac gtcgtcagcg aaataaacca cgccgagatg
     3241 cataacgcca tcgtgcaggc gcagcatgag ctcgcggtcc ggtttgactt caaggggacg
     3301 cgcgcgtcca tcgaattcaa caagaaggag aatcaactca ccctcaccgc cgatcacaag
     3361 ggccagctcg acacggttct ccagatggtc aaggaaaaga tggcccgccg gggagtggcg
     3421 gtcaacgcac tgaagcgcgg caagatcgag gacgccaccc atgattcggt tcgcgagatt
     3481 ctgacgctcc ataccggcat cgagagcgag gatgcccgca agttggtcaa ggagatcaag
     3541 cagctcaaga tcaaggtgca ggcgcagatc atggacgaaa aggtgagggt cacgggcaag
     3601 aagctcgacg agttgcaggc ggtgattgct cacctcaaag cccatggacc ggaatacccg
     3661 ctccagttcg tcaactttac ctgactgaca ggacgtcgcc gaagcgcaga atctggagag
     3721 aacggtcatg aagggcgctg gagagtcagg tgcgagttac ccgatctact aatgacctgg
     3781 actggaccgg cgcattttcg tcgaacgagc gcgcgtccaa gccgcacgcg aggtctctag
     3841 gagaatcgat catgggtttc tggaacggac gcgtcacctt cacgcggtat caagtctctg
     3901 gcgaaaagcc tgttccgttc ggcgagccgg tcattgacca ggcccgggct cacttgatcg
     3961 gccagcacgg tgcgcccgac ccggccgacg gggtatccat cggctggggc ggcggggaac
     4021 acttgctcga cgtggagttg gacggtggta agaacatcgt cgacgacgcc cttcacctgg
     4081 cgatccgcat cgacaccaac aaggttccag gcacgttgtt gcgagcttat acccagatcg
     4141 agattgatgc gcgcgcccag ttaaacccaa gcggcatcgc gaccaagatc cagcgccagg
     4201 aagccaagga agccgccaag ctccgcgccg atgctgaagc cgccgacggc cgttttcgcc
     4261 gtcgcaacca ctttccggtc ctctgggacg gcaggaacaa catcctgtac gccggcagca
     4321 ccagcacgac cgtgctcgac cgcctgcagt cgctcttccg cgagacattc gaccgcacgc
     4381 tcgagccgat cacggccggt atcatggcca agaacctgga attgaacagc ccggagggac
     4441 cgggccggcg gccatcgaac ttgcgtgaac gaaggttcgg gattgtcggc gggacggaaa
     4501 actcaggaat ttcggtcgcc tggtcggagg acgatcccga cagccttgat tatcttggca
     4561 atgagttcat ggtttggctc tggcacgcgc tccaaaacga tggtgattcg gtaaagctag
     4621 ccgacgagtc gcatgtcacg gtcatgctgg ccaagacgct gactctggat tgtcctcggg
     4681 gcgaaatggg ccgagaccat ctcaccgacg acgagccgat ccggctgcca gaggcgttcc
     4741 gggcattgca agcaggcaag ctaccgcgca aggcgggaat ggtacttgtc cgtcacggcg
     4801 ccgactacga gttgacgctg caagccgaga cactggcggt cacggggggc gcgctgccca
     4861 aacccgagga tcggcggctc tcggttcatg atgcgcggat cgcacgcctc gaaggggtcc
     4921 gtcacctggc tgaaactatc gacctgctat tccaggcgta cctcgacagg cgcacgtcgt
     4981 cgaactggag cgacgagctg agccgcattc gccggtggct tcaggccgcc tgaactgatt
     5041 tgctattcac caaactgaca ttatcggcca actggcgcgg cggcatttag caaaatccgt
     5101 ataatcggag tattgatggt cgccaatctg gcgagagttt gcacccggga taccatcgcg
     5161 accttataac ggtttatgcc gattgtacga gagggag
//
LOCUS       DATAQL010000880         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2532735, whole genome shotgun sequence.
ACCESSION   DATAQL010000880 DATAQL010000000
VERSION     DATAQL010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532735"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VHS97_15240"
     CDS             <1..182
                     /locus_tag="VHS97_15240"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEX3449607.1"
                     /translation="DGQVGRNADRENHATVAIEEHERVKLTERDSCLVTELLVNPPAP
                     NAKLRKAAGTMPGRS"
     gene            complement(480..1346)
                     /locus_tag="VHS97_15245"
     CDS             complement(480..1346)
                     /locus_tag="VHS97_15245"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX3449608.1"
                     /translation="MTHLASPQSDLFTRADLAAAALLAAAPISPRLAVVLGSGMSELA
                     DRITEATAVPYDQIPHFPTTTVAGHEGKVLLGKLGGVPVIMFQGRFHFYEGHDLETVT
                     FPVRVLSRLGVSTLILTAATGGIRADLRAGNLVLLTDHLNLIGSNPLRGLNDIRLGTR
                     FPDMTEVYSKRLRAIAREEGKRLGIDLAQGVYACLPGPSYETAAEIKMLRTLGADVVG
                     MSTVPEAIVARHAGIEVLAFALVSNAAAGVVGTPITHEEVLEAGRKAAPTLARLIERV
                     VGRLAGADGAAP"
     gene            complement(1343..>2802)
                     /locus_tag="VHS97_15250"
     CDS             complement(1343..>2802)
                     /locus_tag="VHS97_15250"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEX3449609.1"
                     /translation="TGIFVLIVAAIAGRAGRADDTPGRPVAQPAGDARKDPKAKEKPA
                     PEPGTRVVAGPVEVERAYRLSLGPWSWQFSAPESIDLPDIVDRIRGLFGMLLILAVAA
                     YLSDNRRAISRRVVLWGLGLQWGFALLVLRIPAGIRFLEHAGNGVKAVLDCALEGASF
                     VFGSALVNPAGPAGFVFAFRVLPTVIFVAALFAVLYHVGVMQWVVRGFAVVMSWLMGT
                     SGAESLNVAASLFLGQTEAPLTIRPYLPRLTRSELLTVMTSGMAHVSGGVMAAYIAFD
                     IEARHILTAVIMTAPGAILMSKMLVPETEVPETLGHVSSAEPTTDANVLDAASRGTRD
                     GLHLALNIAAMLISFLGLVALVNMGLSYLGTSLQGIVGWAMAPVAYLLGVPWEDCKAI
                     GKLLGTRTVLNELIAFKDLGELKAQHVLIERSSVIASFALCGFANISSIGIQLGGIGA
                     LAPERRHELARLGLRALFAGTLANYLSACIAGILL"
BASE COUNT          582 a          904 c          870 g          446 t
ORIGIN      
        1 gcgatggaca agtcgggcga aatgcggatc gggaaaacca cgctacggtg gccatcgaag
       61 aacacgaacg cgtcaagctc accgagcgcg acagctgcct tgttactgaa ttgctcgtga
      121 acccgcctgc accaaacgcg aagctgcgga aggccgcagg tacaatgccg ggcaggtcat
      181 gagcaaagga ctcgccgggc atggcgtgcc ggtctttagg cttgggcgat tggcggtgag
      241 tcgttcggtc cagggacaag ggctgggagg tcaacttatc gtggcggcgg gacggcgatg
      301 tttgctcggt ggtcgtgatg cctccccgcc cggaagatgg gatcagccaa tcggactcgc
      361 ggatcgcccc tggggcttaa tcgggaacgc caggatccgt tggcgcaccc acgtcggatt
      421 acgtgaagat cggccacgtt gacacgatca cttccgcgtt caaaccgagt cgtttcacct
      481 cagggcgccg ccccatccgc gccggccaac ctgcccacga ctcgctcgat cagtcgagcc
      541 aaggtcggcg cggccttgcg ccctgcctcc agtacctctt cgtgagtgat cggcgtcccc
      601 acaaccccgg ccgcggcatt cgagaccagg gcgaatgcca gcacctcgat gccggcgtgc
      661 cgggccacga tcgcctcggg gaccgtcgac atcccgacga cgtcggcccc gagggttcgc
      721 aacattttga tctcggcagc ggtctcataa ctcggaccgg gcaagcaggc gtagacaccc
      781 tgcgcgagat caattcccag acgcttgcct tcctctcggg caatcgcgcg cagccgcttc
      841 gagtagacct cggtcatgtc gggaaaccgc gtgcccaacc gtatgtcgtt cagaccgcgg
      901 aggggatttg agccgatcag attcaaatgg tcggtcaaga ggacgaggtt gcccgcgcgc
      961 aggtcggcgc ggatgccgcc ggttgccgcc gtgaggatta atgtcgatac ccccagcctg
     1021 ctcagaacac gcaccgggaa ggtgaccgtc tcgaggtcat gtccttcgta aaaatggaat
     1081 cttccctgaa acatgatcac aggtaccccg ccgagcttac ccaagagcac tttgccttca
     1141 tgtccggcga cagtcgtcgt cggaaagtga ggaatctggt cgtaggggac agctgtcgct
     1201 tcagtaatcc ggtcggcaag ctcgctcatg ccggagccca gtacaacggc caaccgcggt
     1261 gagattggag ccgcggccag gagcgcggca gcggcaagat cggctcgggt gaagaggtcc
     1321 gattgcggcg aagcgaggtg cgtcataaaa ggatccccgc aatacaagcc gaaagataat
     1381 tcgccaaagt cccagcgaac agggcccgca agcccagccg ggccagctcg tgccgccgct
     1441 ctggcgccag ggctccgatg ccgccgagct ggatgccgat cgagctgatg ttcgcaaaac
     1501 cgcaaagcgc gaagctggca atcaccgatg agcgctcgat caacacgtgc tgggctttga
     1561 gctcacctaa gtctttgaag gcgatcagct cgttcaaaac tgtccgcgtc cccagcagct
     1621 tgccgatcgc cttgcagtct tcccacggga cgcccagcaa gtatgccacc ggtgccatcg
     1681 cccagccgac aatgccctga agcgatgttc ccagatatga caatcccatg ttcacaagcg
     1741 cgaccaagcc caggaacgag atcagcatgg cggcgatatt gagcgccagg tgcagcccgt
     1801 cgcgcgtacc gcgggaggcc gcgtcgagca cgttggcgtc agtcgtgggc tcggccgagc
     1861 tgacgtgacc cagagtctcg ggcacttccg tctcgggtac gagcatcttc gacatcaaga
     1921 ttgccccggg cgcggtcatg atcacggccg tcaggatgtg ccgggcctcg atatcaaagg
     1981 caatgtaggc cgccattacc ccccccgaga cgtgggccat ccccgaggtc atgaccgtca
     2041 gcagctccga ccgcgtgagt cgcggcaggt agggacggat cgtgagcggc gcctcggttt
     2101 ggccgagaaa aagcgaggcg gcgacgttga gcgactcggc gccactggtg cccatcaacc
     2161 acgacatcac cacggcgaaa ccgcgcacca cccactgcat cacgcccacg tgatagagga
     2221 cggcaaagag cgccgccacg aagattactg tgggcaatac gcgaaatgcg aagacaaatc
     2281 ccgcaggtcc cgcgggattg acgagcgcac tgccaaagac aaacgacgct ccctcgagcg
     2341 cgcagtcgag caccgccttg acaccattgc ccgcatgttc gagaaaccgg atgccggcgg
     2401 gaatgcgcag cactagcagg gcaaaacccc attgcaagcc cagcccccag aggacgaccc
     2461 tgcgcgagat cgcccggcgg ttgtccgaca gataggccgc caccgcgagg atcaagagca
     2521 ttccgaaaag accgcgaatc cgatcaacga tgtccggcag gtcaatggat tccggcgcgg
     2581 agaactgcca cgaccatggg cccagcgaaa gacgatacgc gcgctcaact tccaccggcc
     2641 cggcgaccac ccgcgtgcct ggttctgggg ccggcttctc cttggccttc gggtctttgc
     2701 gggcatcgcc agctggctgg gccacgggtc gcccaggggt gtcgtccgcc ctgccggcac
     2761 ggcccgcaat ggcggcgaca atgagcacga agattccagt tc
//
LOCUS       DATAQL010000881         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3449057, whole genome shotgun sequence.
ACCESSION   DATAQL010000881 DATAQL010000000
VERSION     DATAQL010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449057"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VHS97_15255"
     CDS             <1..629
                     /locus_tag="VHS97_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449610.1"
                     /translation="PFPCHAPDHAMFLIEEERIGHTADVINVDQLPFGGFGGQEPLVL
                     LSANLEFIRSLEFDWFCGGHGNIGEKSDVDFYLTYLDDVAALVNEYVPRPSELPDLSL
                     TDYLANGLDLKAKSRLLTQLNNHFGLYEYGKQIVEGVKPFWDGVDPFNVGNIPEWTTK
                     RQQAVDEAVPKIIERLRPKYGRMYAFDDAQPFNVRLMAMDMQPAMSNR"
     gene            721..1566
                     /locus_tag="VHS97_15260"
     CDS             721..1566
                     /locus_tag="VHS97_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3449611.1"
                     /translation="MFGPPRDRSEALAVLRDAVEAGVDHIDTAQYYGPRVVNDLICEA
                     LHPYPPGLAIVTKLGARRGDSGEILLYNEPHQLRSGLEDNLTTLQVDELAAANLRLVD
                     GAPADARFDDQLAALVQAREDGLIAGVGLSNITSKHLLRALKITEIVCVQNLFNPADL
                     SSLPLLRECTARGIAFVPFFSLGSGMSRSNAVLGDVTLRAIAARLGATPAQVALAWAL
                     DLAPNVLLIPGTSSRHHLAENLAAASVALDDVSRYELRVGGWPTPSNGTFDRSPRGRG
                     GRFGD"
     gene            complement(1683..1949)
                     /locus_tag="VHS97_15265"
     CDS             complement(1683..1949)
                     /locus_tag="VHS97_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449612.1"
                     /translation="MRLAAGNGGDGTYSAAIAIWAMLVAGTLAQWTAAAIAAGHRVDP
                     TRRVLRLKAVLALAAAAMLALTAARAVVATPLDDLKGVMRPSAF"
BASE COUNT          405 a          656 c          651 g          393 t
ORIGIN      
        1 tcccctttcc ctgtcacgca cccgaccacg caatgttcct cattgaggag gagcgcatcg
       61 ggcacacggc tgatgtgatc aacgttgatc agctgccgtt cggcggattc ggggggcagg
      121 aaccactggt gttgttgtcg gccaacctgg aattcatccg gtcgcttgag ttcgactggt
      181 tctgcggcgg acacggaaac atcggcgaga agtccgatgt cgacttctac ctgacctacc
      241 tggatgacgt cgcggcgctc gtcaacgaat acgtcccccg gccgtcggaa ttgccggacc
      301 tctcgctgac cgactatcta gcaaatgggc tggaccttaa agccaagtct cggctgctaa
      361 ctcagctcaa caatcacttc ggcctctacg agtacggcaa gcagatcgtg gagggcgtta
      421 aacccttttg ggatggtgtg gacccgttca acgtcggaaa catccctgag tggacgacca
      481 aacgccagca agccgttgac gaggccgtac cgaagataat cgagcgactg cgaccgaaat
      541 acggacgcat gtacgccttc gacgacgcgc agccgttcaa cgtccggctg atggcgatgg
      601 acatgcaacc agccatgtcg aatcggtagc gcgacgagaa tgacggaatc acgcttccat
      661 gtcggcccgt tctcagtgag ccggccgggt ttcggtgcaa tgcaccttgc ctgatcgggg
      721 gtgttcggcc cgccgcgcga tcgttcagaa gccctcgccg tgctgcggga cgcggtggaa
      781 gccggagtgg atcatatcga caccgcccag tactacggcc cgagggttgt caacgacttg
      841 atatgcgagg cgttgcatcc ctacccgcct ggattggcga tcgtcacaaa attaggagct
      901 cgacgcggtg actctggcga gatcctgctc tacaacgaac cccaccagct tcgtagcggc
      961 ctcgaggaca acttgaccac cctccaggta gacgagctcg ctgccgccaa tctgcgcttg
     1021 gtcgacggtg cgccagccga cgcccgcttc gacgaccagc tcgcggccct tgtccaggcc
     1081 cgtgaggacg gcctcatcgc tggtgtcggg ctgagcaaca tcacttcgaa gcacctcctg
     1141 cgagcgctga agatcaccga aatcgtttgc gtgcaaaacc tcttcaaccc ggccgacttg
     1201 tcttcgttgc ctctcctgcg ggaatgtacc gcccgaggta tcgcctttgt gcccttcttc
     1261 tctctgggtt cagggatgag ccggtccaat gcggtgttgg gggatgtgac gctccgcgcc
     1321 atcgctgcac gactgggagc cacgccggct caggtggcct tggcatgggc gctcgacctc
     1381 gcccccaacg tgctcctgat ccccggcaca tcttctcggc accacctcgc agagaacctg
     1441 gccgcggcaa gcgtggcgct cgacgacgtc tcccgatacg agctgagggt tggtggctgg
     1501 ccgacgcctt cgaacgggac gttcgacagg tcaccccgag gacgaggcgg aagatttggc
     1561 gactgatgag ggcaacgagg cgcacaacca tggagagagt cttgccctgg tgccatggag
     1621 agagtcttgt cctggtgcgc tgccacgtca gccaaatgag catgtctaac gatcatccca
     1681 ggtcagaagg ccgacggccg catgacaccc ttcaggtcat cgagtggagt tgcgactacg
     1741 gcgcgggccg ccgtcagggc cagcatcgct gctgccgcga gggccaacac tgccttgaga
     1801 cgcaagactc ggcgagtagg gtcgaccctg tgtccggcgg ctattgccgc cgcggtccac
     1861 tgagccaagg tgccggcgac gagcatcgcc catattgcga tagctgccga gtaggtcccg
     1921 tcgcctccgt tgccggcagc gagtcgcacg gcggaccatc ggcgatcaat caaggagctt
     1981 ggcgtaggag gtcacctgac aaccgcggac tgggtggatg agcgacagcc gagggcttca
     2041 attgccgccg atcatgagcc gcaggggtga gatcgtggcc aaccggcatg atcacgatgg
     2101 atcgg
//
LOCUS       DATAQL010000882         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5435350, whole genome shotgun sequence.
ACCESSION   DATAQL010000882 DATAQL010000000
VERSION     DATAQL010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5435350"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1259)
                     /locus_tag="VHS97_15270"
     CDS             complement(183..1259)
                     /locus_tag="VHS97_15270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449613.1"
                     /translation="MKFRPNHTLRRAMIHPSRPWQWSAVNRSLLALLALALGGCVAMS
                     RPADQPQDEPAAGAQNDSKTAMPNQARVSDPKTQFQEKATDRQRFQVHIDFGRVFETQ
                     GNIDAAILEYQDALAVVQSKRRGTLHPADEALAHRRIGGALDRQGRFAQAETHYKKAL
                     KMSPKDPKIWNDAGYSYYLQGRWNDAERALKTATRLSPDDDRIRTNLGLTLAAAGRTD
                     EALPLLSKSDGDAIGHANLGYLLAASGQVDLARRQYERALALRPDLQLARRALEKLDL
                     QERSSQSPGAPNVGVAQATRSTAYPIDAHVTQASTSGVKIPSPVAGDLVPVAKAAAVH
                     TGQPELSSSTTSPSSSDLPLPPPL"
     gene            complement(1261..1890)
                     /locus_tag="VHS97_15275"
     CDS             complement(1261..1890)
                     /locus_tag="VHS97_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449614.1"
                     /translation="MDKRASHIFRWSCVAATLGLAPSAVAQVPVESDGQAVVCSRRGR
                     LHRLFHHSAHTLQDKFVGYPENFAEPPLGSYINEQFAVQVAKADTHRFTLYRSDFLPG
                     TSQFSPVGASRYNIMATRMPGWVGPIRIEWTPEQPELAQARRRAIVETLNKAGQPIVA
                     DRVVIAPSPYPGAMGIEAMNNFNNTIARTQLAAPTFALPPIETAASGVR"
BASE COUNT          336 a          595 c          620 g          409 t
ORIGIN      
        1 gaagtcatcc aggcggaaat cgacccggcc atcgcgcggc gcaaacatct ggtgcgcgta
       61 ccgggaaggc acgagatcga tcggatcgcc gatcgccgtc cgcgatttta ccagcccctc
      121 gtcgcgccca acggtcgcga ctgagtcatc tacaaaatcg ttcagtgatt ccggcgatta
      181 attcacagcg gcggcggcaa gggcaagtcc gatgagctcg ggctggtggt cgatgacgac
      241 aactccggct gcccggtgtg cacagcggct gccttggcca cggggacgag gtcgccggcc
      301 acgggcgacg ggattttcac cccgctggtc gatgcctggg tcacgtgagc gtcgatcggg
      361 taggccgtag atcgcgttgc ctgagcgacg ccaacgttcg gtgcgccggg ggactgcgag
      421 ctgcgctctt gaaggtcaag tttctccagc gcgcgccgcg ccagttgcag atcaggtcgc
      481 agagccagtg ccctctcgta ctggcggcgc gccaggtcga cctgtcccga cgcagccagc
      541 aagtagccaa gattcgcgtg accgatcgca tcgccatcgg atttactcaa gagtgggagc
      601 gcctcatcgg ttcgccccgc ggctgcgagc gtcaggccga ggttggtacg gattcgatcg
      661 tcgtcgggcg ataatcgcgt ggccgtcttg agcgctcgct ccgcatcgtt ccaccgcccc
      721 tgcaggtagt agctgtagcc ggcgtcgttc cagatcttgg gatctttcgg gctcatcttg
      781 agtgctttct tgtagtgtgt ctctgcctgg gcgaatcgtc cctgccgatc caaagccccg
      841 ccaatgcggc ggtgtgcgag cgcctcgtca gccgggtgca aggttcctcg ccgcttgctc
      901 tggacgaccg cgagggcgtc ttgatattcg aggatagccg cgtcgatgtt tccttgagtc
      961 tcgaagaccc ggccaaagtc aatatgcacc tgaaatcgtt ggcgatcggt ggctttctct
     1021 tgaaactggg ttttgggatc agacacgcga gcctggtttg gcatcgctgt cttgctgtcg
     1081 ttttgagcgc ccgctgccgg ctcgtcctgc ggctgatccg ctggacgtga catcgccacg
     1141 catcccccga gcgccagggc caagagcgct agcaatgagc ggttcacggc cgaccattgc
     1201 caggggcgac tggggtggat catcgctcgt cggagagtgt gattcggtcg aaacttcatg
     1261 tcatcgaact ccgctggcgg ctgtctcgat cggcggcagc gcaaacgtcg gtgccgcgag
     1321 ttgcgtacgc gcgatcgtgt tgttgaaatt gttcatggcc tcgataccca tcgcacctgg
     1381 atacggggat ggcgcaatga ccacgcgatc cgcaacgatt ggttggccgg ccttattcag
     1441 cgtttcgacg atcgcccggc ggcgcgcctg ggccagctcg ggctgctccg gagtccattc
     1501 gatcctgatc ggcccgaccc agcctggcat tcgagtcgcc atgatgttgt accgtgacgc
     1561 tccaaccggc gagaactggc tggttcccgg caggaaatcg ctgcggtaaa gagtgaatcg
     1621 atgcgtgtct gccttggcaa cctgcaccgc gaattgttcg ttgatataag agccaagcgg
     1681 tggctcagcg aaattctcgg ggtaaccgac aaatttgtcc tgcagcgtgt gcgcagagtg
     1741 gtgaaagagc cggtgcaacc gcccgcgacg gctacacacg acggcctggc catcggactc
     1801 cacgggaact tgcgctaccg cacttggcgc cagcccgagt gttgcggcga cgcacgacca
     1861 ccggaagata tggctcgctc gcttgtccat ggttacctct atacctttca gtttccttta
     1921 ccgtcggcga attgtctcga ttagtcggca tgaccatgcg
//
LOCUS       DATAQL010000883         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_550795, whole genome shotgun sequence.
ACCESSION   DATAQL010000883 DATAQL010000000
VERSION     DATAQL010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550795"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VHS97_15280"
     CDS             <1..754
                     /locus_tag="VHS97_15280"
                     /inference="COORDINATES: protein motif:HMM:NF016165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEX3449615.1"
                     /translation="KSGFFPQMASLGSGSDFTAFLDHLAIPAVEVGFSGGYGVYHSIY
                     DSFNWMEKFGDPEFLTHAMAARLYTLIAMRAAAADVVPIKFVPYGQALREHVDQLRLI
                     HVRRTRKADPGKTKARPEFEGLSSLVEAVCAFQVQADELDRATNAVTERGQFSGDELA
                     GLNHALAQVERAFLLDKGLPDRPWFKHAIYAPGLTTGYAAWPLPAIRQALEDDSKVRL
                     AADLPATVERIKSATAALERARKLAQIVLETH"
     gene            835..2337
                     /locus_tag="VHS97_15285"
     CDS             835..2337
                     /locus_tag="VHS97_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3449616.1"
                     /translation="MTQVEREDRRLVTLPRVLGPIAAFCVVVGSVIGSGIFLVPATVA
                     QNVPFISGIILVWIIGGLFSGAGALTLAELGAMLPRAGGLYVFLRVAYGALPAFLFGW
                     VEFLVVRSGSMATLAAAFARYFARLVPPPELIRTELWQAGAATLAIAVVTAVNVMGTR
                     RGGTLQVFGTVLKVGGVMVLITLPFVLGRGTIANLSPIWPSAVGGSIFTGMMTAMIGV
                     LWAYDGWTNVTPLAEEIRDPGRNVPRALICGMAVLIGVYLSMTLAYHYVLPMSEVASA
                     SSNDVRSAVAALYCDHLLGWPGVVAISVLVMCSTFISLNGNALTGPRAYFAMARDGLF
                     PVGLCRVHDRFQTPANAILAQGIWAIVLTLAGTFLILVSPAVAPGLPRLILAAWKKLN
                     ETPLYDLLYTYVIFGANLFYMLAISSVFVLRVRRPELPRPYRTWGYPFTPLLYVAAAV
                     LLLGNMLADHQSRVQSLAGLGIILLGVPAYWLFRVSATDANEAGQPQTGG"
     gene            complement(2400..3806)
                     /locus_tag="VHS97_15290"
     CDS             complement(2400..3806)
                     /locus_tag="VHS97_15290"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449617.1"
                     /translation="MRTKRAILLGLYLCLVTGPSALLSRCLLAAPRSPVGESSGGDKA
                     VPSYCKDVAPILQKHCLDCHRKGQVAPFSLETYEQVRKRASDISSVVEDRVMPPWKAV
                     PHFGLTLRGDRSLSSAEIATITQWADADAPEGNPADLPPPRQFPDGWSLGTPDLVLDT
                     GTDFAVPASGEDVYRCFVVPTDLPDDVYVSGIIYRPGNRRVVHHILSYVDTSGEGKKK
                     DALDAAPGYPCFSGPGVDSIGDMGGWVPGIEPTRLPDGFGKALPRKADIIVQIHYHPS
                     GKPETDRTQVGLYFAKKPVKRTLQRAGAWNPDLVLPIDGPNPSHIEAKASWTVPVDII
                     AFACAPHMHLLGRDMQISVRFPDGRRQDLLKIDDWDFNWQFAYRFAVPKILPKGTVLD
                     VVAHYDNTADNPRNPNKPPKVVRWGEATRDEMCIGFIMIAKKDQDLTKPGEKDDLFKI
                     IKQSGGWPILSDKPSGKR"
BASE COUNT          636 a         1114 c         1259 g          833 t
ORIGIN      
        1 caagagcggc ttctttcccc agatggcctc gctggggtcg ggatccgatt tcaccgcatt
       61 cctcgatcat ctggcaatcc cggcggtaga ggttggtttc agcggcgggt acggagtgta
      121 tcactcgatc tacgacagct tcaactggat ggagaagttc ggcgatcctg agttcttaac
      181 gcatgcaatg gcggcccggt tgtacacgct gatcgcgatg cgggctgccg ccgccgacgt
      241 ggttccaatc aagttcgtcc cctacggcca agccctgcgt gaacacgtcg accagctcag
      301 gctcattcat gtccggcgaa cgcgtaaggc tgatccgggc aagacgaagg ccaggcctga
      361 gtttgaaggg ctgtcaagcc tggtcgaagc ggtgtgtgcc ttccaagtcc aggcggacga
      421 gctcgatcgg gcgaccaatg cggtcacaga gcgtgggcaa ttctccggcg acgaactggc
      481 cgggctcaac catgcgctcg cgcaggtcga gcgagcgttc ctcttggaca agggcttgcc
      541 ggatcgccca tggttcaagc atgcgatcta cgcgcccggc ctgaccaccg gttacgcggc
      601 gtggcccttg ccggcaattc ggcaggcgct cgaagacgat agcaaggtgc gactcgccgc
      661 cgatctgccg gcgaccgtcg agcggatcaa gagcgcgacc gcggcgctcg agcgagcacg
      721 caagctggcg cagatcgttc tcgagacgca ctgatataag ggttaagtcg cctttttatc
      781 cgagtctttc aattcccaat tctatgcggc aaagatccgc gaggaccttg cgtgatgacc
      841 caagtcgaaa gggaggatcg ccggttagtc acattgccac gggtgctggg gccaatcgcc
      901 gccttctgcg tggtcgtggg ctcggtaatc ggttccggaa tctttctggt tccggcgacg
      961 gtggcgcaga atgtcccgtt catcagcggc atcatcttgg tatggatcat cggtgggctc
     1021 tttagcggag cgggggcact cacgcttgcc gagctgggcg cgatgctgcc gcgcgcgggg
     1081 ggcctttacg tgtttctccg cgtagcgtac ggagcgctgc cggcgtttct gtttggctgg
     1141 gtcgagtttc tcgtggttcg ctcggggtca atggcgacac tggccgcggc gttcgcacgg
     1201 tacttcgcgc ggctggtgcc gccgccggaa ttgattcgca ccgagctatg gcaggcgggc
     1261 gcggccactc tcgcgatcgc cgtggtcacg gcggtcaacg tgatggggac gcgtcgggga
     1321 gggacgcttc aggtctttgg cacggtcttg aaggttggcg gagtcatggt tttgatcacg
     1381 ctcccgtttg tcctgggccg tgggacgatc gccaatctga gtccgatctg gccgagcgcg
     1441 gtcggcggtt cgatcttcac gggcatgatg acggcgatga tcggcgtact ctgggcgtac
     1501 gatggctgga ccaatgtgac gccgctcgcg gaggagatcc gcgatccggg ccgcaacgtc
     1561 ccccgtgcct tgatctgtgg gatggccgta ttgattggcg tctatctgtc tatgacattg
     1621 gcctatcact atgttctgcc gatgagcgag gttgcctcgg ccagtagcaa tgacgttcgg
     1681 agcgccgtcg ccgcccttta ctgcgaccat cttttggggt ggccgggcgt cgtggccata
     1741 tcggtcttgg taatgtgttc gacgttcatt tcgctcaacg gcaatgccct gaccggtccg
     1801 cgggcttatt tcgccatggc gcgcgacggc ctctttccgg ttgggctctg ccgggttcac
     1861 gaccgctttc aaacgccggc caatgccatc ctggcccagg gaatctgggc gatagtcttg
     1921 acgctcgccg gcacgttttt gatcctcgtt tcccccgcgg tcgcgcccgg tttgccgcgc
     1981 ttgatcctgg cggcctggaa aaagctcaat gaaacgcccc tctacgacct gctttacacc
     2041 tatgtcatat tcggagccaa cctgttttat atgctggcga tctcgagcgt gttcgtcctc
     2101 cgcgtgcgcc ggcccgaatt accgcggcct tatcgcacct ggggctatcc gttcaccccc
     2161 ctgctctatg tggcggcggc ggttctgctt ctgggaaaca tgcttgccga tcatcagagc
     2221 cgggttcaat cactggcggg gttgggcatc atcctcctgg gcgtgccggc ctactggctt
     2281 ttccgggtga gcgccactga cgcgaacgaa gcgggccaac cgcagacggg agggtgacgc
     2341 gcggtcggag aacgggcggt gtggcccata ttgtcactgt tgttatgatt aaccggtggc
     2401 tatcgcttgc ccgacggctt gtcgctcaag atcggccagc cgcccgattg tttgatgatc
     2461 ttgaagaggt cgtccttctc cccgggcttg gtgagatcct gatctttctt ggcgatcatg
     2521 atgaagccga tgcacatctc atcccttgtc gcctcgcccc agcgaacgac tttgggaggc
     2581 ttgttgggat tgcggggatt gtccgccgtg ttgtcgtaat gggccacgac gtcgagcact
     2641 gtgcccttgg gcagaatctt tgggaccgca aagcggtaag cgaattgcca gttgaagtcc
     2701 cagtcgtcga ttttgagcaa atcttggcgg cggccgtcgg gaaagcggac cgatatctgc
     2761 atgtcgcggc cgaggaggtg catatgggga gcacatgcaa atgcgatgat atcgactggg
     2821 accgtccacg acgctttggc ttcgatgtgg gacgggttgg gtccatcgat gggcaggacc
     2881 aggtcagggt tccaggcgcc ggcgcgctgg agcgttcgct ttacgggttt cttggcgaag
     2941 tagaggccga cttgcgtccg gtcggtttcg ggtttgccgc tgggatggta gtggatctgc
     3001 actatgatgt cggccttgcg cggcaaggct ttgccgaagc cgtcggggag ccgcgtgggc
     3061 tcgatgccgg gtacccaacc tcccatgtcg ccgatgctgt ctacccctgg gcccgaaaag
     3121 cacgggtagc ccggcgcggc gtcgagggcg tctttcttct ttccctcgcc ggaggtatcg
     3181 acataagaca ggatgtgatg cacgacccgg cgattgccgg gcctgtagat gattcccgat
     3241 acgtaaacgt cgtcgggaag gtcggtcgga accacaaaac aacgatagac gtcttctccg
     3301 gatgccggca ccgcgaagtc ggtgcccgtg tcgaggacca ggtcgggagt tcccaagctc
     3361 cagccgtcgg gaaactggcg cgggggcggc aggtcggcgg ggttgccctc aggcgcatcg
     3421 gcgtcggccc attgggtgat ggtcgcaatc tcggcgcttg acagcgacct gtcgcccctg
     3481 agagtcaatc cgaagtgcgg caccgccttc cacgggggca tgacgcgatc ctccaccacg
     3541 ctcgagatgt ccgaggcgcg cttgcggact tgctcgtaag tctcgaggct aaacggcgcg
     3601 acctggcctt tgcggtggca gtccaggcaa tgtttctgca agatcggcgc gacgtccttg
     3661 caatagctcg gaacggcttt gtcgccgcca gacgattcac caaccgggct tctgggggcg
     3721 gccagaaggc aacggctcaa gagggccgat ggacctgtaa caaggcacaa ataaagcccg
     3781 agcaagatcg cgcgctttgt tcgcatttgg tttctccaag agcagtgttg attccgacgc
     3841 gg
//
LOCUS       DATAQL010000884         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_91236, whole genome shotgun sequence.
ACCESSION   DATAQL010000884 DATAQL010000000
VERSION     DATAQL010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91236"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VHS97_15295"
     CDS             <1..773
                     /locus_tag="VHS97_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3449618.1"
                     /translation="VVATGIPVARIIPGVGVELEGGERIEARCVVSNADPRVTLRLLG
                     DAADPVWQSHVEAVPQVGCTVKLNVALNELPSFTARPGTNMPHHLCQINTPLSKEEWR
                     THYQAARAGELPARLWTELYFHSAHDRTVVPAGVHTMSVFAQYVPHTFARGDWETRRD
                     DVKDLALRSIARYCNNMPAAVIDVQVLGPPDIEREVGLTGGHIFQGECLPAFMWDKRL
                     EPRTSMPGVFLCGACTHPGGSVIAINGRNAAMEILTGT"
     gene            complement(814..1746)
                     /locus_tag="VHS97_15300"
     CDS             complement(814..1746)
                     /locus_tag="VHS97_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX3449619.1"
                     /translation="MRTVITGGAGFIGSHLCERFVGDGDEVICVDNLLTGSRRNIAQL
                     VDNPSFRFVEHNISEAVEIDGPVDNVLHFASPASPVDYLTHPIPTLKVGSLGTHNALG
                     LAKAKDARFLLASTSEVYGDPEVHPQREDYWGNVNPIGPRGCYDEAKRFAEAITMAYH
                     RYHGVKTRIVRIFNTYGPRMRLNDGRVLPNFMKQALSGEPLTIYGKGEQTRSFCYVTD
                     LVEGIVRLLKTDFSEPVNLGNPTEITVMQLAEEIISLIGDTKSKIVYEDLPQDDPKRR
                     KPDITRAQTLLGWQPVVDRAEGLKRSLEYFRRVV"
     gene            complement(1888..2631)
                     /locus_tag="VHS97_15305"
     CDS             complement(1888..2631)
                     /locus_tag="VHS97_15305"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HEX3449620.1"
                     /translation="MLASGMPEPQCWPDTRSILPMNSSTTSQTGEQQEPGGQSLGLFS
                     SRCGADRASDGRPTAPSRILFLDDDPARAESFLSENPQAIWVQTVEECLARLVEAWDE
                     VHLDHDLGGQVFVDMNKTDCGMEVIRWLCKERRDHLSSTRFFVHTHNSLAGLLMVLQM
                     QSIGYRAEFRPFGVDPALFLPGADHGPPIDGCDRAHNESPPPALSGRPLWGALHRWWG
                     RLRACWNFSKAAHKSSASLDKGRTEAGRP"
     gene            complement(2633..3013)
                     /locus_tag="VHS97_15310"
     CDS             complement(2633..3013)
                     /locus_tag="VHS97_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449621.1"
                     /translation="MAGCERGYLCTVCGREVEEITDSDLYLRYVLGEAEWNELNRAAE
                     RHIRCNPVLAQFVVDPSFEPMLCAGAFSKTHLDPEFVRSEENRVTSAFLRLRELVAEN
                     LPIADYPLPDVIARRLSGSVTSDT"
     gene            complement(3159..>3499)
                     /locus_tag="VHS97_15315"
     CDS             complement(3159..>3499)
                     /locus_tag="VHS97_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3449622.1"
                     /translation="IERHHKTKKDAPSGTALRLAETAGRGQMSDHLAPAEGGPANAHP
                     PGEISVHSLRLADSPGEHVVVFGLMGETLELSHRALNRDGFVRGAIDAAKFLAGKPAG
                     LYTIEDALGC"
BASE COUNT          717 a         1089 c          997 g          696 t
ORIGIN      
        1 ccgtggtcgc gaccggcatt ccagtggccc ggatcatccc tggcgtgggc gtcgagctgg
       61 aaggtggaga gcggatcgaa gctcgttgcg ttgtctcgaa cgcggacccg cgcgtgacac
      121 tgcggctgct gggcgatgcc gccgacccgg tctggcaatc acatgtcgaa gccgtccctc
      181 aggttggatg taccgtaaag ctcaatgtgg ctctcaacga gctaccgagc ttcacggccc
      241 gacccggaac caacatgccc catcacttgt gccagatcaa cacgccgctc tcgaaggaag
      301 aatggcggac ccattatcag gccgcgcgtg ccggcgagct gcccgcgcgg ctctggactg
      361 agctctattt tcacagtgcc cacgaccgca ccgtcgtgcc cgccggcgtg cacacgatga
      421 gtgtttttgc ccagtatgtt ccccacacgt tcgcacgagg agactgggaa acccgccggg
      481 atgatgtgaa ggacctcgcg ctgcgttcga tcgcgcgcta ttgcaacaat atgcccgctg
      541 ctgtcatcga cgtgcaagtc ctcggtcccc ctgacatcga gcgcgaagtc ggcttgaccg
      601 gcggccacat cttccagggt gaatgcttgc cggccttcat gtgggacaag cgattggaac
      661 cgcgcacgtc catgcccgga gtcttcctct gcggcgcctg cacacaccct ggcggcagcg
      721 ttatcgcgat caacgggcgt aacgcggcca tggaaatact gaccggcaca taggggcagc
      781 agcagtatcc gaacatattc gatcgcacaa catctaaaca accctgcgga agtactcgag
      841 cgagcgcttc aggccttccg cacgatcaac cacgggctgc catcccagga gcgtctgggc
      901 gcgggtaatg tcaggcttac gccgtttcgg atcgtcttgc ggcaggtctt catagacgat
      961 cttgctcttg gtatcaccga tcaaggagat gatctcttcc gcgagttgca tcacggtgat
     1021 ttcggtggga ttgcccagat tcaccggctc cgaaaaatcg gtcttgagca ggcgcacaat
     1081 gccttcgacc agatcggtaa cgtaacagaa actgcgcgtc tgctcgccct tgccgtagat
     1141 cgtcaggggt tcaccgctca aagcttgttt catgaaattg ggaagaacac gcccgtcgtt
     1201 gagccgcatt ctggggccgt aggtgttgaa gatccgaacg atccgagtct tgaccccatg
     1261 atagcggtgg tacgccatcg tgatcgcctc ggcaaagcgc ttggcttcgt cgtaacagcc
     1321 ccgcggacca atcggattga cattgcccca gtagtcttca cgttgaggat ggacctcggg
     1381 gtcgccgtag acctcagacg tgctggccag cagaaaccgc gcgtctttgg ccttggcgag
     1441 cccgagcgca ttatgtgtgc caagcgagcc aaccttgaga gttgggatag ggtgagtcag
     1501 gtagtcgacc ggactcgctg gactggcgaa atgcagaacg ttgtcgaccg gcccgtcaat
     1561 ctcgaccgcc tcgctgatgt tgtgctcgac gaaccggaat gaggggttgt cgacaagctg
     1621 cgcgatgttg cggcggctgc cggtcagtag attatccacg cagatgactt cgtcgccgtc
     1681 acccacgaat cgctcacaaa ggtgcgaccc gatgaaaccc gctcctccgg taatgactgt
     1741 tctcacggcg agacctccag tcctcggcaa tcgacaacgg acatcaaatc acaccgacga
     1801 gaactaacat acaatttcgt caggcccatt gacaattgaa tccgactcgt tcgcccggtc
     1861 ggggcgtcgc ctcggctatc ccaagtttca cggccggccc gcttcggttc gccctttgtc
     1921 taggcttgct gaggatttat gggctgcttt tgagaagttc cagcacgctc tgagccggcc
     1981 ccaccatcga tggagcgcac cccatagcgg ccgacccgat agtgcgggcg gtggcgattc
     2041 attgtgggct cgatcacacc catcaattgg cggcccgtgg tctgcgccgg gaagaaacag
     2101 ggccggatct actccaaacg ggcggaactc ggctcggtat ccaatcgatt gcatctgcaa
     2161 gaccatcaac agtcccgcga gcgaattgtg cgtgtgaaca aaaaaacggg tcgagctcag
     2221 atgatcgcgc cgctccttgc aaagccaccg gatgacctcc atgccgcaat cggttttatt
     2281 catatcgacg aagacctgac cacccaggtc gtgatcgaga tggacctcgt cccaggcttc
     2341 gaccagccgg gcgagacact cctccactgt ttgaacccag atggcctggg ggttttcgct
     2401 caagaaagat tccgcacgcg cggggtcgtc atccagaaag agaatccggc ttggcgcggt
     2461 tggccggccg tctgacgccc ggtccgcccc gcaccggctc gagaacaacc ccagggattg
     2521 ccctcccggc tcttgctgct cacccgtttg actcgtggtc gaggaattca tgggaagtat
     2581 cgatcgagtg tccggccagc actgaggctc gggcattccg ctggccaaca tttcaagtgt
     2641 cactcgtaac tgaacccgac agccgccgcg cgatcacatc gggcaaagga tagtccgcaa
     2701 ttggcaaatt ttccgccacg agctctcgca acctgagaaa cgccgaagtc acgcgatttt
     2761 cttcgctcct gacgaactcc ggatccagat gagtttttga aaaggcaccg gcacataaca
     2821 ttggctcaaa cgatgggtcc acgacgaatt gagccaagac gggattgcag cgaatgtgcc
     2881 tttccgctgc gcggttcagc tcgttccatt ctgcctcgcc cagcacgtag cggaggtaca
     2941 ggtcggagtc ggtgatctct tcgacttccc gcccgcagac ggtgcacaga tatccccgtt
     3001 cgcatccagc catacttcac cctcgccgaa caagccgtct tcgccgcaag acctatcgcg
     3061 cattcacgta gtaagagcaa acttcgttca cgctgccagc gaagtctaca tcatgcgaaa
     3121 agccgcacac ttctaagtgt actcgatatc gccaagattt agcacccaag ggcgtcttcg
     3181 atagtgtaca aacccgcggg tttaccagcc aggaacttgg cggcgtcgat ggctccgcgg
     3241 acgaaaccat cgcgattgag tgcccggtga ctcagctcca gggtttcgcc catcagtcca
     3301 aaaaccacga catgctcgcc cggagaatcc gcaagccgca gcgaatggac gctgatctcg
     3361 cccggcggat gcgcgttggc ggggccaccc tcggcaggag ccaggtgatc ggacatctgc
     3421 ccccggccag ccgtctcggc cagccggagg gccgtcccgc tgggcgcgtc cttcttggtt
     3481 ttatgatgcc gttcgatga
//
LOCUS       DATAQL010000885         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5435433, whole genome shotgun sequence.
ACCESSION   DATAQL010000885 DATAQL010000000
VERSION     DATAQL010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5435433"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..1314)
                     /locus_tag="VHS97_15320"
     CDS             complement(364..1314)
                     /locus_tag="VHS97_15320"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3449623.1"
                     /translation="MSWTLWSWLAAPAIWQPDWKGLGVALIKGREYLAAGSQRTSLDI
                     YLPDTHFDKPVSKQGRPAVLVIHGGSWIGGSAAAWRADPSEVVIRLAQQGLAVFAVDY
                     RLARPGKPSWPAVVADLREAVRWVRRHSDEFGVDSGRIAVLGVSSGAHLAALLGTQPE
                     EQGPDGVSSRVQAVVSFYGPSDLARLMVARRLFHEPVRMFMGDGPSRSADLVAEASPI
                     SHVTHDDPPFLLLHGSDDHWVSLDQSVRMASTLAIAGVPHRLIVVPGARHGFGLTVEY
                     PSHRDLLPEIVGFLESVWNISSVAAQKSRRCCWYGACTFL"
     gene            1521..>1909
                     /locus_tag="VHS97_15325"
     CDS             1521..>1909
                     /locus_tag="VHS97_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX3449624.1"
                     /translation="MDAQKLRLPLLDQREIEARIVGPLVRAFASQLGNDQALTIVGEV
                     VRELARQSGGELARLLGEQSLEAFAQSLGRWRENGALEIDILEQTPERLSFNVTRCRY
                     AEMYRDLGLADLGASLSCQRDYALAEG"
BASE COUNT          414 a          590 c          573 g          332 t
ORIGIN      
        1 cgatcggcgg ccgtgtgcaa gacacgatcg aattccccgc tgagctcgat ggcccccggg
       61 atcgacgaac tcaggccgcc gacatgaatc tcggtcgaca cggcgcaacc tcctccagac
      121 ggcttaagac ccccgccacc aaaacgggtg tcttgcggat cccttcatga ttgtgtgtgc
      181 gtgaatgaga ttgggtttcc taatccttcc aagttgtccc aagaaattca aggatctcga
      241 gaatcaaatt cgaataatca gccgggaagt ccggatcgtc gccgccgggc accaaagcct
      301 ggtcgtgatg tatccttgcc acctcaagag tcctcgacgt caatcgggac ggctggtcgg
      361 cgatcacaaa aaggtgcaag ccccgtacca acaacatcgg cgggactttt gtgccgctac
      421 agacgaaata ttccacacac tttccaagaa accaacgatt tcgggcagca agtcgcgatg
      481 cgagggatat tccactgtca gtccgaaacc gtggcgcgcg ccgggaacga caatcaggcg
      541 gtgcggaacg ccggcgatcg ccagtgtgga agccatccgg accgactggt ccagtgacac
      601 ccagtggtcg tccgagccat gaagcaagag gaatggtgga tcgtcatgcg tgacgtggct
      661 gatcggtgac gcctccgcca ccagatccgc cgacctcgat ggtccgtcgc ccatgaacat
      721 gcgcaccggt tcgtgaaaaa ggcggcgcgc aaccatcaat cgggcaaggt ccgagggacc
      781 gtaaaaactc acgacggcct gcacccgcga tgacacaccg tcggggcctt gttcctccgg
      841 ctgagttccg agcagggccg ccaaatgcgc tccggacgag acgcccagca ccgcaatccg
      901 gccggaatcg acgccaaact cgtcgctatg ccggcgcacc catcgaacgg cttcgcggag
      961 atcggccaca accgccggcc aactgggctt gccgggccgg gcgagccgat agtcgaccgc
     1021 gaaaacggcc agtccttgct gggccaaccg gataacgact tcggatgggt cggctcgcca
     1081 agcggccgcc gagccgccga tccagctacc gccgtgaatc acgaggacgg cgggacgccc
     1141 ctgcttggag acgggcttgt cgaaatgcgt gtctggtaga taaatgtcca gcgacgtccg
     1201 ctgtgagccg gcggcgaggt attcacgtcc ttttatcagc gcaacgccca gacctttcca
     1261 gtccggctgc catatcgccg gcgctgccaa ccacgaccaa agggtccatg acaacacact
     1321 aatccataca atgaggcggg acacaatcgc gattgtgcgc atcgagcggc tcggatcatg
     1381 ggatcgggtc agcaatggag ccctccatgg tacaatcggg cttcaggaaa aggatgtggg
     1441 atgaacgatg aatttcagga cacgacacgc cagggctgat caacttattc tccgtcttag
     1501 atgccgtgag gctgcgcgac atggacgctc aaaaactgcg gttgcccctg ctcgatcagc
     1561 gcgagatcga ggcacgaatc gtgggaccct tggtgcgggc gttcgcctct caactaggga
     1621 atgaccaagc gctcacgatc gtcggcgagg tcgtgcgtga actcgcccgt cagagtggtg
     1681 gtgagctcgc tcgactcctc ggcgagcagt cgctggaggc ttttgcgcaa tccctcggcc
     1741 gatggcgcga aaacggcgct ctcgagatcg acatcctcga gcaaacccca gagagactgt
     1801 cgttcaacgt cacgcggtgc cgctatgccg agatgtatcg cgacctggga ttggccgacc
     1861 tgggcgcgag cctgtcctgt cagcgggact acgctctggc cgagggatt
//
LOCUS       DATAQL010000886         4221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1160679, whole genome shotgun sequence.
ACCESSION   DATAQL010000886 DATAQL010000000
VERSION     DATAQL010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160679"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VHS97_15330"
     CDS             <1..702
                     /locus_tag="VHS97_15330"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449625.1"
                     /translation="GWASMILPDLEQQSLHDRIVFETPLQDPSMATTRLVPLNVFLCP
                     TDNMPLRWTATESDTWLYMGKIYSTALPICDVAGSNYVGVFGIAEPGVNGEGVFSRGS
                     YMPLTAITDGLSQTLCVGERSENLQQGRGMATWVGSAPGANVWSCAPNPYEPDAGTCV
                     KEDGSGMILGHTGEGHGPGDPYGDVNQFISRHGGGCFFLYCDGHVQFLRKEMNYQVYK
                     ALSTRDWGEIISDGY"
     gene            692..1060
                     /locus_tag="VHS97_15335"
     CDS             692..1060
                     /locus_tag="VHS97_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449626.1"
                     /translation="MAIKPSAEQMIFESAARQGGPPRPTPLLKWAAVVAVVCAVQGCG
                     EHDEPPEMTKQAIKIDEVPDTVRTAAKKAIPGVNLNEAWKNLERGGKLHSYEIRGKNS
                     ADGKTREVRVSLTGEILESE"
     gene            complement(1032..1505)
                     /locus_tag="VHS97_15340"
     CDS             complement(1032..1505)
                     /locus_tag="VHS97_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX3449627.1"
                     /translation="MTIWTRLLRWFFVVALTIYVGGFTFYSVVVIPILHDRLESAFLA
                     GLVTQRVTDALNLLGVATLSLGWCVYCVRAMDRLRGNLLSHWDFWPLMISSIGLVVLL
                     VLHRLLDRKLETGTLAEFYPFHRAYLWVSTVQWFANLALLTQSTGAFTPTPESRR"
     gene            complement(1800..1991)
                     /locus_tag="VHS97_15345"
     CDS             complement(1800..1991)
                     /locus_tag="VHS97_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449628.1"
                     /translation="MITAVVLIFVLVFLIWPESQAKLELQLRQRLPRLYLLTFHSIKR
                     YHIDMQNRFPDRSRPKRLR"
     gene            complement(2084..2485)
                     /locus_tag="VHS97_15350"
     CDS             complement(2084..2485)
                     /locus_tag="VHS97_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449629.1"
                     /translation="MGRILRIMTEGSVEQASFDPNEIGPVRDMSSLGEQASPDQDQAG
                     LEDAELNKIKELPKELGVMLLTAGIVGFILPGPGTPAIIAGGLALWPEAFGKLESWLE
                     RSHPKVHRQGVRQINRFLNDLEKRYPYSNPS"
     gene            complement(2487..>4221)
                     /locus_tag="VHS97_15355"
     CDS             complement(2487..>4221)
                     /locus_tag="VHS97_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449630.1"
                     /translation="RGQLGLPALYTSIVAATLASGQFIASAAMNLMLTFWNRRYATEL
                     TSARRRLLGRIAQQPRFVRLATPEPDGTLVEVLVDDLKPHDVILVSAGERIPADGQVK
                     KGQALVDERLVRGIDGLSSKQPDDVVFAGSTVRLGELHIEVLRVGADTQAAALFRATL
                     GATPALRDSATPSEHGAPFAEPVVAPTMALAGIGLLVGDISMAGAILRPDYATGPGLA
                     FPLEAVQAGALCFQNGILIRDPSALDRLAEIDLVILDHHAALERALLAVDAIEVFPDY
                     AENDLLRYAATALHELDDPRVAALLDACTACGVELLALQPAEFATDLTLLHGSDRIKI
                     GDLGPRARGHISANQQSSHERNESAAPSSLMVGINGRVAGLIHFRRSERLEAAAALLR
                     LRSRTNLNIGLVSDQPEPRAAALRVSLGVDFHVGALSAHDRIRLLRDCRHRGIKVAYV
                     GDCRIDPRTAALAHVAISLGSRDVSDLAYDPAPICLLQPRISKLNALWEIALVHRRRV
                     TVAHGYSLIPNLACVAGAFAWGFTSLASVLVTNLGTYSVYSRTAASIRSLERQFARSP
                     QRPRSGARGES"
BASE COUNT          885 a         1265 c         1305 g          766 t
ORIGIN      
        1 ggttgggcca gcatgatctt gcccgacctc gagcagcagt cgcttcacga tcgcattgtc
       61 ttcgagacgc cgctacaaga tccgagcatg gcgaccactc gtctggtgcc attgaacgta
      121 ttcctgtgcc ctaccgacaa catgcctctc cgctggacgg ccactgaaag tgatacatgg
      181 ttgtacatgg gcaagatcta ttcaacagcg ctccccatct gtgacgtggc aggctctaat
      241 tatgtcggtg ttttcggcat tgccgagccg ggcgtcaatg gtgaaggcgt cttctcccga
      301 gggagctaca tgccgttaac cgcaatcacc gacggcttga gccaaacgtt atgtgtgggg
      361 gaacggtcgg agaatctgca acaaggacgt ggcatggcga cctgggttgg gtcggctccg
      421 ggcgcgaatg tgtggtcgtg cgcgcccaat ccctatgaac cggatgccgg aacctgcgtc
      481 aaggaagacg gctcgggcat gattctgggc cacactggcg aggggcacgg gccgggcgac
      541 ccgtacgggg acgtcaacca attcatcagc cgccacggcg ggggatgctt tttcctctac
      601 tgtgacggcc atgttcagtt cctgcgaaaa gagatgaatt accaggtcta caaagcgctc
      661 agtacacgcg actggggtga aatcatttct gatggctatt aagccctccg ccgagcaaat
      721 gatattcgag agtgccgcgc gacaaggcgg gccgccacgt ccgacaccgc tcctgaagtg
      781 ggcggccgtt gtcgccgtcg tctgcgccgt tcagggctgt ggcgagcatg atgagccgcc
      841 cgagatgacc aagcaagcga tcaagatcga cgaagtcccc gatacggtgc gcacagccgc
      901 gaaaaaggct atcccgggag tcaacctcaa cgaagcgtgg aaaaacctcg agcggggcgg
      961 aaagcttcac tcctacgaaa ttcggggcaa gaactctgcc gacggcaaga cacgggaggt
     1021 tcgcgtatcg ctcaccggcg agattctgga gtcggagtga acgctccggt cgattgggtt
     1081 aagagtgcga ggttggcgaa ccattgcacc gtgctcaccc acagatatgc ccgatgaaaa
     1141 gggtaaaact ccgcgagtgt gccggtctcc agcttgcgat ccagcaaccg gtgcaacacc
     1201 aacaagacca cgaggccgat cgacgaaatc atcaatggcc aaaaatccca atggctcagt
     1261 agattgcctc gaagtcgatc catcgcgcgg acgcagtaga cgcaccaccc cagggacagg
     1321 gtcgccacac cgagcagatt gagggcatcg gtaacgcgtt gcgtaaccaa tcccgctaga
     1381 aaagcgcttt ctaaccggtc atgcaagata gggatcacga ccacactgta aaacgtgaat
     1441 ccccccacgt agatcgtcag agccacgacg aaaaaccacc gaagtaatct ggtccagatc
     1501 gtcatcgtgg cggagtctcc tggcggattc aggggggtat agcggcagag tactactcta
     1561 tggatccgtg ccaaccaaga ccattggcga ttttcgttgc gccgcctggg cgagggccgc
     1621 tttccccccc ttgtgaaggg tcgggtggtc agcgagcgat cagccacggt gtggtcaacg
     1681 cgaagttagc atccgccaag ttgccttcca gtcgagcgca acaataacgc gtagttcgca
     1741 accgccggtc tgggccagcc aacatcaacc tttcatcagc caaaacgcgc ctctcgccgt
     1801 caccgcagtc gtttcggccg cgaccggtcg ggaaagcggt tttgcatgtc gatgtgatag
     1861 cgcttgatcg aatggaacgt gaggagatag agcctgggca ggcgctggcg caattgcagc
     1921 tcgagcttgg cttgcgactc gggccagatc agaaaaacga gcacgaagat cagtacaacc
     1981 gcagtgatca tcagacgtgt cctctcgata gcgcgccaga cgctaaccac cacccgaatg
     2041 aacttcgctc attggtaatc aaggcgagaa cgaggtcgat ccctcagcta gggttcgagt
     2101 agggatagcg tttttcgagg tcgttcaaga aacggttgat ctggcgcacg ccctgtcggt
     2161 gtactttggg gtgagagcgc tcaagccaag attccagctt gccaaaggct tccggccaga
     2221 gcgccagtcc gccggcgatg atggccgggg tcccgggtcc cggtaaaatg aagccgacga
     2281 ttcccgccgt caagagcatg actcccagtt ccttgggaag ctccttgatc ttgttgagtt
     2341 cagcgtcttc cagaccggct tgatcttggt ccgggctcgc ttgctccccc agactgctca
     2401 tgtcccgaac cgggccaatt tcattggggt cgaaggatgc ctgctccaca cttccctccg
     2461 tcatgattct cagaatgcga cccacgtcag gattctccac gagcacccga cctggggcgc
     2521 tggggcgacc gggcgaactg gcgctcgagg ctcctgatcg aggcggccgt tcgcgagtag
     2581 acgctgtacg tgcccaggtt ggtcaccaag accgacgcga gcgatgtgaa accccaggcg
     2641 aaagcccccg cgacacaggc aagatttggg atcagtgaat acccatgagc aaccgtcaca
     2701 cgtcgccggt gaacaagcgc gatctcccag agcgcgttta gcttcgagat tcgaggctgt
     2761 aagaggcaga tgggcgccgg atcatacgcc aaatcgctca cgtctctgct accgagtgaa
     2821 atggccacgt gcgccagggc cgcggtgcgc gggtcgatcc tgcagtcacc aacgtaggcg
     2881 accttgatac cgcggtggcg gcagtcgcgc agcaagcgga ttcgatcgtg ggcggacagg
     2941 gcgccgacgt gaaaatccac ccccagcgaa acccgcagcg cggctgcgcg cggctccggc
     3001 tggtccgaga cgaggccgat attgaggttg gtccgcgacc gcaggcgaag cagagccgcg
     3061 gcggcttcta gccgctctga ccgtcggaaa tggatcaggc ccgcaacacg accgttgatg
     3121 ccgaccatga gcgaactggg cgcggcggac tcgttgcgct cgtggctgct ttgctggttt
     3181 gcgcttatgt ggccgcgggc acgcgggccg agatcaccga tcttgatgcg atcactcccg
     3241 tgcagcaagg tcaagtcggt cgcaaactct gccggctgca gagccaagag ttcaacgccg
     3301 caggcggtgc acgcatccag gagagcggcg acgcgcgggt cgtcgagttc atgcaatgcg
     3361 gtggcggcgt agcgaagcag gtcgttctca gcgtagtcgg gaaatacttc aatggcgtcg
     3421 actgccagta gggcacgctc gagggccgcg tgatggtcga ggatcaccag gtcaatctct
     3481 gcgagccggt cgagcgcgct tggatcgcgg atcaagatgc cgttttgaaa gcagagagcg
     3541 ccggcctgaa cggcctcgag tggaaacgcc agacccggcc cggtcgcgta gtcagggcgg
     3601 aggatagccc ccgccatcga gatgtcccca accaagagtc cgatgccggc caaggccatc
     3661 gtcggcgcga cgaccggttc cgcgaagggg gcgccatgct cgctgggggt tgccgaatct
     3721 cgcagcgccg gcgtcgcccc tagtgttgcc cgaaacaagg ccgctgcctg tgtgtctgcg
     3781 ccaactcgca acacctcgat atggagctca cctagtcgca ccgtcgagcc ggcgaagact
     3841 acgtcatcgg gctgtttgga cgacagtccg tcaattcccc gcaccagccg ctcgtcgacc
     3901 aaggcctggc cttttttgac ctgtccgtcc gctggaatcc gttctccggc cgatactagg
     3961 atcacgtcat gcggcttgag atcgtcgacc aggacctcga caagcgtgcc gtccggctcg
     4021 ggtgttgcca gccggacgaa gcgaggctgt tgcgcgatcc ggcccagcag ccggcgacgg
     4081 gcgctcgtca actcggtggc gtaccgccga ttccaaaagg ttagcatcaa gttcatcgcg
     4141 gccgatgcga tgaattggcc gctcgccagg gttgccgcga caatgcttgt ataaagcgcc
     4201 ggcagcccca actgcccgcg g
//
LOCUS       DATAQL010000887         6728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4364628, whole genome shotgun sequence.
ACCESSION   DATAQL010000887 DATAQL010000000
VERSION     DATAQL010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4364628"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..331)
                     /locus_tag="VHS97_15360"
     CDS             complement(161..331)
                     /locus_tag="VHS97_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449631.1"
                     /translation="MVLLLVFIPTCLTGKSTLDRSRSANSGDGPVAIMATEVLDGLQK
                     TGLVVIVALTLL"
     gene            517..1377
                     /locus_tag="VHS97_15365"
     CDS             517..1377
                     /locus_tag="VHS97_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX3449632.1"
                     /translation="MEVLACLNGEIMPVEQARVPVWDRGFLFGDAVYEVWRTYRGRCW
                     LEREHLARLKRSLKELDFAPVDLERLMDRLHRTLRASQIQEGTVYVQITRGVAPRAHV
                     FPDPAVPPTELIIARPYDDGPTARLRASGAATISHPDLRWKRCDVKSTNLLGNVLALE
                     TARRAGCQEAVLVDSAGLVTEATHSSLIWARDGRLEGTPEGHEILPGMTRQHILRLAD
                     KIGISFVSTRVTLPQLLAADEVILFGTTIEVFPVVRVDDHSIGGGRPGPIACRLDGAF
                     KDELERWLAS"
     gene            complement(2496..3587)
                     /locus_tag="VHS97_15370"
     CDS             complement(2496..3587)
                     /locus_tag="VHS97_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3449633.1"
                     /translation="MLQSSPLIQLYLVRLRDFYRQPARVFWVYGFPTLLAIALGFAFQ
                     NRPPAPVLVDLVQGPAESRIKAALEEHNAALDRDHKAGKPRQNVPSVEIHEAPEEEAL
                     KRLNTGRTPIVIVPLGTDTWSYRFDPTRPEASAARQTIDDILQRAANRHDPIQTKDEY
                     VSEPGSRYIDFLIPGLIGLNAMGGGLWGIGFLLVNFRIAKLLKRFVATPMPRSDFLLA
                     LLGARLTFVIPDLAVLLLLGKLMFQMPIRGNLALVIGIELLGALAFAGIGLLVACRVN
                     TTEAVSGLMNLVMLPMWLFSGVFFSSDRFPPAAQPFIQALPLTQLVGALRRVLLEGAT
                     LLDVGTSVLILAAWAVVTFVLALRFFRWT"
     gene            complement(3650..4663)
                     /locus_tag="VHS97_15375"
     CDS             complement(3650..4663)
                     /locus_tag="VHS97_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3449634.1"
                     /translation="MAECAIRVKDLRKEYPSRSGTVHAVSGIDLEIKQGECFGLLGPN
                     GAGKTTTVEILEGLNQPTSGEVEVLGLEWQTDEVAIRERIGVTLQETRFPEKETVREI
                     VTLFRSFYKAGMNPDDVIERVSLESKARTFIEQLSGGQQQRLAVALALVGDPEVLFLD
                     EPTTGLDPQSRRQLWDVIRDLRDRGRTVVLTTHYMDEAERLCDRVAIIDHGKIIAQGS
                     PVELIARLGGDHIIEFALELSGAAAKQEADSFLGLDSVLAARIENDGYSLTVAQPHRA
                     IPALLDELDQRQLPLIRLTTRHVSLEDVFVSLTGRHLRDDETKEGPDAAAAGGRKNRW
                     KRA"
     gene            complement(4767..6053)
                     /locus_tag="VHS97_15380"
     CDS             complement(4767..6053)
                     /locus_tag="VHS97_15380"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX3449635.1"
                     /translation="MPRVISLILGGGRGTRLYPLTKSRSKPAVPIAGKYRLIDIPVSN
                     CIHAGLTRIFVLTQFNSVSLHRHIYNTYKLDPFSGGFVEILAAQQTMEHESWYQGTAD
                     AVRRNIPYFTESSYDLVLILSGDQLYRMDFQDMIRTHLENKAVVTIAALPVAEHEAKA
                     CGIMKIQADGRVLDFEEKPKTPEKLNQVRTDPGWLERLGLNAAGRPYLASMGIYLFNR
                     ALLVDLLKSTTATDFGHELLPQTIAAHRVQAHLFDGYWEDIGSVGAFHQANIELTAEN
                     PKFDFASDAGPIFTRPRFLPCSRIAGATVKNSLICDGSVIGRGSVIENSVIGVRARVA
                     ENVTIRDTYIMGIDFLENPNQLAENQRLGRPQFGVGPNSVIERAIIDKNARIGRNVKV
                     VNDRGTLESEDQITHVIRDGVVVIPKSTVLADGTSI"
     gene            complement(6201..6524)
                     /locus_tag="VHS97_15385"
     CDS             complement(6201..6524)
                     /locus_tag="VHS97_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6793 family protein"
                     /protein_id="HEX3449636.1"
                     /translation="MPLFEVETTSHIMIACADNEQTARAFAATHYPIEEIIRVTQRPR
                     DAWVISKKLLGIHPEGDPCTTARDCLSSAQGDKLHAVRLYMQKTGSDLEGARKVIESN
                     MVRGW"
BASE COUNT         1330 a         1990 c         2048 g         1360 t
ORIGIN      
        1 caaccggatt gttgctgtgc tcgagtctct cgaccaaaac ctggcaactc gccagcccgc
       61 cgtagaccag ccagaagatt acgccaatct cggccgcaat cagcagtccc gataggctct
      121 cgtggaacag cggccagacg acgcgcacaa gctttcggaa ttacaagaga gtgagggcca
      181 cgatcacgac gagccccgtc ttctggagac catcgagcac ttcggtcgcc atgatcgcga
      241 ctggcccgtc cccggaattc gccgatcgcg accgatcgag cgtcgacttg cccgtcaggc
      301 aagtcggaat gaacaccagc agcaatacca tgagtccaag aggccagagc ttacgggtgt
      361 cgctaaaaca acggggtccg atcccttatt gcggggagtg cacttcgggt cggccggccg
      421 tgttcggtgg cattgccgac cagatgacca ccgggttatc ctggctaaca ctttgtgctg
      481 tccttcatgc ctttcccgta gaaagggtcg cggtcgatgg aagtattggc ttgtctcaat
      541 ggcgagatca tgccggtcga gcaggctcgc gtgccggtgt gggatcgtgg cttccttttt
      601 ggtgacgcag tctatgaagt ctggcggacg tatcggggac gttgctggct cgagcgtgaa
      661 cacctcgcgc gactcaagcg cagtctcaag gagctcgact ttgcgccggt cgacctggaa
      721 agattgatgg atcgcctgca ccgcactctc agggcgagcc agatccagga gggcactgtt
      781 tatgtgcaaa tcacgcgcgg agtcgcgccc agggcccatg tgttccccga tccagccgtc
      841 cctcccaccg agctgatcat cgcccggccg tacgacgatg gcccgactgc gcgactgcgt
      901 gccagcggag cggctaccat cagccatccc gacctgcgct ggaaacgatg cgacgtcaaa
      961 tcgacaaacc tcctcggcaa cgtgctggcg ctcgagaccg cccgccgcgc cggttgtcag
     1021 gaagccgtgc tggtcgattc ggcggggctg gtcaccgagg ccacgcattc gtccttgata
     1081 tgggcgcgtg acggtcggct ggagggaacg cccgagggcc atgagatttt gccgggtatg
     1141 acccggcaac atatcctgcg cctcgcggac aagatcggta tctcatttgt ctccacccgc
     1201 gtgacgcttc cgcaattatt ggctgcggac gaggtgatct tgttcgggac gacgatcgaa
     1261 gtctttccgg tcgtacgcgt cgacgaccat tcgatcggcg gcggtcggcc cggacccatt
     1321 gcgtgccggc tggacggagc ttttaaggac gagcttgagc ggtggctggc aagttaacca
     1381 ccagccgtct gaccgcgccc ttccgccccg cctccccttc gagggagggg ttttcgtcat
     1441 cactggctcc ctctccttgg ggagcgggct ggggtgaggg gttgattgca gcaacaccgc
     1501 ccctcgatcg aatgactcgc ctcgagagat ggtggccagc gtgagagcgg tggcgccgcc
     1561 tcctacgagc tcccactcct cggcgagagg ggtgtagtga ggcgcgggtt gatgaaggaa
     1621 cgataatcgc cgagaatgag agcaaggtga cagattccca gagagaggga gccagtaaca
     1681 agcatcggcc gcgagtatgg tgtagcggcg tcgggcttcg cccgcaatcg gccgtataac
     1741 cggccataac atgcatcggc cgctagggtg ctatagcggg gcctatggcc tccccggcag
     1801 ttgtcagaaa aacccctccc tctgagcccc gcctccccgg agttcgcatg accacgaccg
     1861 aggccagcga cgaccctttc accgaacacc agcggcgcga tccattgcct gacgcgggaa
     1921 tccgggtcgt gatgctcacc gacgtgccag ccgaaagcgc tgcgggcgtg atggagccac
     1981 tggcggaatt gatcgctgcg tcgggccgac cggtcgaacg acgaatcgtt gcggttagtc
     2041 gggaacaagg cacgggtgaa gcactgaagc gagggctgcg gggcgcccgc ttgccgctcg
     2101 tactcgtgac caccgcatgt gagctttgga ccaaggcaca cctggaaccg cttcttcacg
     2161 ccatcgacgg gtgtgaccat gtgatcggct gccgcctcaa gagccgccgc gcggcctgga
     2221 ccgacccggt cggatcgctg acgcgccggt tagttttcgc tctcccactg cgcgacgtct
     2281 actcgccgtg tcggctacat cgtctcgata agcttgaagg catgcctttc caatcggtat
     2341 cgtcgttcct cgatgtcgaa attctcgcca aggccacctt tctcggccac ttgatcgacg
     2401 aggtggaggt ccctcctctt ggtggcctta cggtttcggc gggatggtgg acggattgga
     2461 atcgaatcct tcgtcacccg aacttcgtcg gatcatcagg tccagcggaa gaagcgcaat
     2521 gccagtacga acgtgacgac ggcccaggcg gcgaggatca agacggatgt gccgacatcg
     2581 agaagagtcg ccccctcgag cagcacccgt cgcagcgcgc cgaccagctg agtgagtggc
     2641 agggcctgta tgaacggctg ggcggccggg gggaaacggt ccgaggagaa gaatacgccg
     2701 gagaatagcc acattggcag catgaccagg ttcatcagcc cgctgaccgc ctctgtggtg
     2761 ttcacgcggc aggcaaccaa gaggccgatt ccggcgaatg ccagcgcgcc caagagctcg
     2821 atgccgatca cgagggccag gttgcctctg atcggcattt gaaacatgag ctttccgagg
     2881 aggagcagta cagccaggtc ggggatcaca aacgtgagcc gagcccccag cagagcgagc
     2941 aagaaatcgc ttctaggcat gggggtggcc acgaaccgct tcaagagctt ggcgattcgg
     3001 aagttgacca gcaaaaagcc gatgccccaa agtccccctc ccatcgcatt caacccgatc
     3061 aggcccggaa tgagaaagtc gatgtagcgc gagcccggct cgctgacata ttcgtctttt
     3121 gtctgtatgg gatcgtgccg gttggcggcg cgttgaagga tgtcgtcgat cgtctgacgg
     3181 gcggcgctgg cttctggccg cgtcgggtca aagcgatagc tccacgtgtc ggtgccgagc
     3241 ggcacaatca cgattggtgt ccgacccgta ttcagtcgct tgagggcctc ttcctcaggt
     3301 gcctcgtgaa tctcgacgga tggaacattc tggcgtggtt tgcccgcttt gtggtctcgg
     3361 tcgagagccg cgttgtgctc ttcaagcgca gccttgatcc gagattcagc cggcccctgg
     3421 accaggtcga ccaagacagg tgcgggggga cgattctgga aggcgaatcc cagcgcgatc
     3481 gccagcaatg tgggaaagcc gtaaacccag aaaacgcgcg cgggctggcg gtagaaatcg
     3541 cgaagtcgaa ccaggtagag ctgaatcaac ggtgaagatt gtaacatgga attttcgtaa
     3601 gtttgttgcc agtgcctcgg gggcgaaagt gccggcccaa aggggaatgc tacgctcgct
     3661 tccagcgatt cttccggccg ccagcggcgg ccgcgtcggg gccttctttg gtctcgtcgt
     3721 cgcgcaggtg gcggcccgtc agggaaacga acacgtcctc caggcttaca tgccgcgtcg
     3781 tgagtcgaat caagggcagc tgcctttggt caagctcgtc aagcaaggcg gggatcgcac
     3841 gatgcggttg tgcgactgtg agcgagtatc catcattttc gatccgggct gcgagcaccg
     3901 agtcgaggcc gaggaacgaa tcggcttctt gcttcgccgc cgcgccggaa agttcgagcg
     3961 cgaattcgat aatgtgatcg ccgccaagcc gggcgatcag ctcgacaggt gaaccctgag
     4021 caatgatctt tccatggtca atgatcgcca cgcggtcgca gagccgctcg gcctcgtcca
     4081 tgtagtgtgt cgtcaacacg accgtccggc ctcgatcgcg caggtcgcga atcacatccc
     4141 agagctgccg ccgagattgg gggtcgagcc ctgttgtcgg ctcgtccagg aacaggacct
     4201 ctggatcacc caccaaggcc agggcgaccg ccaggcgttg ctgctgcccg ccggagagct
     4261 gctcgatgaa ggttctggct ttgctttcca aagagactcg ctcgatcacg tcgtcaggat
     4321 tcatgccggc cttgtagaag cttcggaaca gcgtgacgat ctcccgaacc gtctcttttt
     4381 caggaaaccg cgtttcttgc agtgtgaccc cgatccgttc ccggatagcg acttcgtcgg
     4441 tctgccactc cagtcccaaa acttccactt cgccggacgt cggttgattc aaaccttcaa
     4501 gaatctcgac ggtcgtggtc ttgcccgcac cattggggcc aagcaagcca aagcactcgc
     4561 cctgcttgat ctcaaggtcg ataccgctta cggcatggac agtgccgctg cggctggggt
     4621 attccttccg caagtccttg acgcgaatcg cgcattcggc cataccccgg tcgcctccca
     4681 gtcgtcaatt ggatcaatca gaaacggtgc cgttccggat tcctgttccg agcttttgta
     4741 tgcgcgaaag cggaatcgga aatctctcag atcgacgttc catctgccag tacggtcgat
     4801 ttggggatca ccacgactcc gtcgcggatc acgtgggtta tttggtcttc ggactcgaga
     4861 gttccccggt cattgacgac cttgacgttg cggccgatac gggcgttttt gtcgatgatc
     4921 gcccgctcga tgaccgaatt cgggcccaca ccgaactggg gtcgtcccag tcgctggttt
     4981 tcggccagtt gattgggatt ttcgaggaaa tcgatcccca taatgtatgt gtcgcgaatg
     5041 gttacgtttt cggcgactcg tgcccgcaca ccgatgaccg agttctcgat gaccgagccg
     5101 cgaccgatca cggaaccgtc gcaaattaag ctgttcttca cagtggcgcc cgcgatgcgc
     5161 gaacagggga gaaatcgagg ccgcgtaaaa atcgggccgg catccgaggc gaagtcgaac
     5221 ttggggttct cggcggtgag ctcgatattg gcctggtgga acgccccgac cgatccgatg
     5281 tcttcccagt agccatcaaa caggtgggcc tggaccctgt gggcggcgat cgtctgcggc
     5341 aagagttcat ggccgaagtc ggtggccgtg gtcgatttca acagatcgac caagagagcg
     5401 cgattgaaga gataaatgcc catgcttgcc aggtaaggcc ggccggcggc attcaatccc
     5461 agccgttcca gccagccggg atcggttcgg acctggttta acttttcggg cgtcttgggc
     5521 ttctcttcaa agtcgagaac gcggccgtcc gcctggattt tcatgatgcc gcacgccttg
     5581 gcctcgtgtt cggccacggg tagggccgcg atggtcacga ccgccttgtt ttcgagatgc
     5641 gtacgaatca tgtcctgaaa atccatccgg tagagctgat ctcccgaaag gattaaaacc
     5701 aggtcgtaac tgctctcggt gaagtaggga atgttgcggc gaacggcatc ggcggtcccc
     5761 tgataccatg actcgtgctc catcgtctgc tgagccgcca gaatctcgac gaagccgccg
     5821 ctgaagggat cgagcttgta ggtattgtaa atgtgccggt gcaaactgac agaattaaat
     5881 tgggtcaata caaagatccg ggtcaacccc gcatgtatgc aattggaaac ggggatatcg
     5941 atcagtcgat acttgccggc gatcgggacg gccggcttgc ttcgggattt cgtcagcgga
     6001 taaagccgcg ttccgcgacc accgcccagg atcaacgaaa tgacccgagg catagcaatc
     6061 ctcctcacgg ataattaacc agcccgccat tgcggtccgg accgcgaccc caagtgcgcc
     6121 gagttcgccc tgcgccacca ttacgcaacg ctatcgtaca ctctcgcgcg aaactcgacg
     6181 agtcgagcct ggcctttatc tcaccaaccc cggaccatgt ttgactcgat gacctttcgt
     6241 gcaccttcca ggtcgcttcc cgtcttttgc atgtagaggc ggacagcgtg caacttgtcc
     6301 ccctgggcgc tcgagagaca atcgcgtgcg gtcgtgcagg gatcaccctc cgggtgaatt
     6361 cccagaagtt ttttcgagat cacccacgcg tcgcgcggcc gctgagtgac tcggatgatc
     6421 tcctcgatcg gataatgcgt cgccgcgaaa gcacgtgcgg tttgttcgtt gtcggcgcac
     6481 gcaatcatga tatgtgatgt cgtctcgacc tcgaagagcg gcatgattgg ttccctttcc
     6541 ggaaatgtat tttcaacgag aagggcctgg accgaaactt tcgcgaatat ctgagagaac
     6601 tgcttaacgg atcgtactcc ttagaaacct tggggtcaaa ggtgaagagc gttctgcggt
     6661 caattgtgag tcgcgaactc agagctgtcc aaaggcaggt tgccagaaag ctcagttatt
     6721 cctttgat
//
LOCUS       DATAQL010000888         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2384187, whole genome shotgun sequence.
ACCESSION   DATAQL010000888 DATAQL010000000
VERSION     DATAQL010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384187"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="VHS97_15390"
     CDS             complement(<1..1228)
                     /locus_tag="VHS97_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX3449637.1"
                     /translation="MNSERWSKIRDLFDASLSLPPSERAPWLRNACGDDPCLRDEVEH
                     LLVHDMQADQDGFLGHPDDENSELGATGDWPPWNNQGPVADGGHRSRQRTSSLDDTEC
                     FSPKAAIASNPWQRSDEETRAVVQSRLRELPIIYVLIFGMMLLIRPIVLSVYPLAMLL
                     PLIIPFVIVSVPLIGIAFLLAARRPSLASLRKTELGMTAALAAILVIYECRAIIGPWL
                     DDRILAEKVMKNVVLLVSLLMVLDAIYIPKGWRRTAIVSSSLAMLPLATLMCVYVVRP
                     ASVSWVGEARSDGIKPLTVFGLDTAFLLTLAVISSFAAHTISLLRSQVAEARRFGQYR
                     LGRRIGAGGMGEVFLAEHELLKRPCAVKLIRPEVVLRAGTIERFEREVRINAKLSHPN
                     TVEIFDYGRTEDGTYYY"
     gene            complement(1225..1806)
                     /locus_tag="VHS97_15395"
     CDS             complement(1225..1806)
                     /locus_tag="VHS97_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3449638.1"
                     /translation="MATEVQAELTIILAKARAGDDGARGEVISKIYEELRHVAARMMH
                     KERASHTLSPTAVVHEAVVRLLGEAVFDRATDRNFLFAAAARAMREILVEHARRRAAA
                     RRGGKWERVPLDSLVDYFEAQDIDVSEIHEALIRLTALDERQGQAITLRYFGGLTVAE
                     IASSLGVSVATVERDCRLGRAWLHTQLRDGRGG"
     gene            2148..2381
                     /locus_tag="VHS97_15400"
     CDS             2148..2381
                     /locus_tag="VHS97_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449639.1"
                     /translation="MAFRIGRDCESPELIAEFPLSPINDDHAYSMAIAILDRLFEAGD
                     RRTPAETEYFHALAKLACEYEEARIKRKGVRKL"
     gene            complement(2441..3118)
                     /locus_tag="VHS97_15405"
     CDS             complement(2441..3118)
                     /locus_tag="VHS97_15405"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3449640.1"
                     /translation="MGATLGTEKPRKGHKGMGMEGAVARWYARNTWKSIEQFRKLARS
                     LAGQLESGSSVLEVAPGPGYLAIELAKEGRFRVVGLDISKTFVAMAAAHAKDVGVQVE
                     FHLGNASAMPFDSNSFDMIVCRAAFKNFAEPLGAIDEMYRVLKTGGRALIFDLRPDAS
                     KADIAAEVDGMKLGWLNSLITRLTLQWLLSRAHSQETFRQMAAQTPFKTCAITEDSIG
                     LEVTLKK"
BASE COUNT          672 a         1034 c          942 g          666 t
ORIGIN      
        1 cgtagtaata cgttccgtcc tcggtccggc catagtcaaa gatttcgacg gtgttggggt
       61 gcgaaagttt cgcgttgatc ctcacctccc gctcgaaccg ctcgatcgtt cccgctctga
      121 gaacgacctc cggacggatc aacttgacgg cgcacggccg ctttaagagc tcatgctcgg
      181 ccaggaaaac ctcacccatc ccccctgcgc cgattcgcct acccaggcga tactggccga
      241 accgtcgcgc ctcggcgacc tgcgatctca gcagcgagat cgtatgggcc gcgaatgacg
      301 agattacggc cagcgtcagc aggaacgctg tgtccaatcc gaagaccgtt agcggcttga
      361 tgccatcgga ccgcgcctcg ccgacccaac tcactgatgc cggacggacc acatacacgc
      421 acatcagagt agccaggggc agcatcgcca gcgacgagga tacgatggca gtccggcgcc
      481 agcctttcgg aatgtagatc gcgtcgagga ccatcaacag cgacacgagc aaaaccacgt
      541 tcttcatgac cttctcggcc aagatccggt cgtcgagcca aggaccaatg atcgcccggc
      601 attcatagat gaccaagatg gccgcgagcg cggcggtcat gcccagctcg gtcttccgga
      661 gtgatgcgag cgacggccgg cgagctgcga ggaggaacgc gatcccgatc aagggcacgg
      721 acacgatgac aaacgggatg atcaacggca agagcattgc caaagggtac acgctcagga
      781 cgatcggccg tatcaagagc atcatgccaa agatgaggac gtaaatgatc ggcagctcgc
      841 gtaatcgcga ctggacgacc gcacgcgtct cttcgtccga tcgctgccat ggatttgagg
      901 cgattgccgc cttcggcgag aagcattcgg tgtcatcaag cgacgacgtc cgctggcggc
      961 tccgatgtcc gccgtcagcg acgggccctt ggttgttcca cggcggccaa tcccccgtgg
     1021 ccccgagttc ggaattctcg tcatcgggat ggccgaggaa gccgtcctgg tcggcctgca
     1081 tgtcatgaac gagcaaatgt tcgacctcat cccgcaggca aggatcgtcg ccgcaggcgt
     1141 tgcgaagcca tggggcgcgc tcggatggag gcaaactcaa cgaggcgtcg aacaagtcgc
     1201 ggatcttgct ccatcgctcg gagttcatcc gcccctcccg tctcgaagtt gggtgtgcag
     1261 ccaggcccgc ccgagccggc aatcacgctc gacggtcgct actgaaaccc ctagtgacga
     1321 ggcgatctcg gctacggtaa ggccaccgaa gtagcgcaac gtgatcgctt gcccctgtcg
     1381 ctcgtcgagg gccgtgagcc gaatcaaggc ctcgtggatc tcggagacgt cgatatcttg
     1441 cgcctcgaaa tagtctacca gtgagtctag tggcacccgc tcccatttgc cgccccgccg
     1501 cgccgcggcc cgccgccgcg catgttcgac gaggatctcg cgcatggccc gcgcggccgc
     1561 cgcgaacagg aagtttctgt cagtggccct atcaaagacc gcttcgccca aaagccgcac
     1621 gaccgcctcg tgtacaaccg cagtgggaga aagggtgtgg ctcgcccgtt ccttgtgcat
     1681 cattcgggcg gccacgtgac gcaactcctc gtaaatcttg gagatgactt ccccgcgggc
     1741 gccatcatcg cccgcgcgcg cctttgcgag gatgattgtt agttcggcct gcacctcagt
     1801 cgccatcgaa cctccaagaa atccttgagg ggtttcggcg tcattattcg cgacatactt
     1861 atagagggca aggaaggcac cggtcggttc ggcgacttcc gatgcgccgg ctgcatccac
     1921 ccacttgcct tggcgtctga cgggggctcg tccaatgatg gaacttgtgt tctggagctt
     1981 tgttgtgaca ctcccgccgc tcgtcctaag tgtcgtggtg agaatcgtct cagtcttcgc
     2041 agatttcttc gctcgatgcg gctggtggcc ggtctccctg catcgacact cgcagccaac
     2101 ctaccattgt gtcggccgcc aggttcgtcg accggagagt ttcgggcatg gcgtttagga
     2161 tcgggcgtga ttgtgaatcc ccggagctaa tcgccgagtt tcctctgtcg ccgatcaacg
     2221 acgatcatgc gtacagtatg gcgatcgcta ttttggatcg cctattcgag gcgggcgatc
     2281 gacggactcc ggcagagacg gagtatttcc atgcgctggc gaagctggcc tgcgagtacg
     2341 aagaggctag gatcaagaga aaaggggtca ggaagctata gatcggtctg aatcctgaca
     2401 cctttctcgc gggctggcga cggcgcaggc cgagctgccg tcactttttg agcgtcactt
     2461 ccagtccgat cgaatcttcc gtgatggcgc atgtcttgaa gggagtctgt gcagccatct
     2521 gacgaaacgt ctcttgcgag tgagcgcgcg aaagcagcca ctgtaaggtc aacctcgtga
     2581 tcagagagtt gagccagcct agcttcatac catcaacctc ggcggcgatg tccgccttcg
     2641 aagcgtcggg ccgcaagtcg aagatgagcg cccttccccc ggtcttgagc acgcgataca
     2701 tctcatcgat cgcgcccaac ggctcggcaa agtttttgaa cgcagcccgg caaacgatca
     2761 tatcaaatga gttagagtca aatggcatcg ccgacgcatt accgagatga aactccacct
     2821 ggacgcccac gtccttggca tgcgccgccg ccattgcgac aaatgtcttg ctaatatcca
     2881 accccactac acgaaagcgg ccttccttgg cgagctcgat cgccaggtac cctggtccgg
     2941 gagcaacttc taatacactc gagccacttt caagctgtcc ggccagcgag cgtgccaact
     3001 ttctgaattg ctcaatgctc ttccaggtgt ttctcgcgta ccaccgcgca acggctccct
     3061 ccatgcccat gcccttgtgg cctttcctcg gcttctcggt tcccagagta gcacccatcg
     3121 aaaacctccg ttacaattct ttccagctac tttactagtt cgctaagtaa cttagacact
     3181 aagatattta gcttctaagc ggattgacct ttcgtgtcaa gagacggtcc cgacaaagcc
     3241 gagctcgtgg ctgagctcgt ggacatcatg cgcgagctca gcacggcgac gattctgttt
     3301 caccaggccg tcgc
//
LOCUS       DATAQL010000889         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5435600, whole genome shotgun sequence.
ACCESSION   DATAQL010000889 DATAQL010000000
VERSION     DATAQL010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5435600"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2981
                     /locus_tag="VHS97_15410"
     CDS             <1..2981
                     /locus_tag="VHS97_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX3449641.1"
                     /translation="IDAVPDLTNVQVQVITEAPALSPLEVDSLLSFPVSGAMSGLPEI
                     EQIRSVSKFGVSVVTIVFKEKTDIYRARQLVGERLPRAAAAIPPGYGTPMLGPIATAL
                     GEVFQFQVKATKESGMTPMDLRTLLDWFIAYQLRGVEGVTEINAHGGEMKTYEVEPDP
                     DKLANHRLSMTNLFDALRSNNANVGGGYLVREGEARYIRGVSQVRTIDDIAAIVIDER
                     DGIPVTVAHVAKVRAAPMIRAGMATRDGEGEIVTGLVMMLIGQNGRDVVTRVKAEIEN
                     LKKSLPPGVTIEPLYDRTHLINQTLNTVLHNLTAGGVLVMAILLAFLGNLRGGLIVAL
                     AIPLSMLFAANVMLATGISASLMSLGAIDFGLIVDSSVIMIENCVRRLAHEGGTRPKL
                     DIVRDAAIEVRKPTMFGELIIAIVYLPILALEGTEGKLFRPMALTVIFALAGSLLLSL
                     TLMPVLAAMGLSSRPQEKEIWLLRWIKHLYQPMLDTFVRHPTAAVFLALGLIGASVPI
                     ALNLGGEFMPRLNEGDLLIEAVRIPAASLEGAALASTQIENLLKTIPEVRLVYCKTGR
                     PEIANDVMGVHQTDVWTMLKPQEEWRPGLTRDALIEEIDKLLTEHVPGVKFGFSQPIE
                     MRVNELVAGVKSDVAVLIHGADLKVLRRLSLEVERVLSQIPGARDIKNPTAGRLPMLR
                     IKVRRDQLARYGIKASDVLDSVAALGGTTVGTVFDDDKRHPLRVRLPESWRNDADKIG
                     SIRVIDPQGWPIPLKDLAEIAEEEGPNEVERENVQHRAVVGANVRGRDIASFVAEAQA
                     AIDEQVKRPYGYVLRWGGQFEHLQTASKRLTIVVPVAMLLIFLLLYSTFHSMRLAFLI
                     YTAVPMAATGGIFSLFLRGLPFSISAGVGFIALFGVAVLNGLVWVSAVEHLRQEGVEP
                     HEAAREAAVVRLRPILMTALVAGLGFIPMAVATTPGAEIQRPLATVVIGGLITSTLLT
                     TLVLPAVYPWFAQKPNVLEA"
BASE COUNT          578 a          897 c          966 g          610 t
ORIGIN      
        1 cgatcgatgc ggtgccggat ttgacgaacg ttcaggtcca ggtgatcacc gaagcgccgg
       61 cgttatcgcc gcttgaggtc gactcgcttt tatcgtttcc ggtctcaggc gcgatgagcg
      121 ggcttcccga gatcgagcaa atccggtcgg tctccaagtt cggcgtttcg gtggtgacga
      181 ttgtcttcaa ggaaaagacg gacatttacc gggcgcggca actggtcggg gaacgtctgc
      241 cgcgcgcggc ggcggcaatt ccgccgggct acgggacacc gatgcttggg ccgatcgcca
      301 cggctttggg cgaggtattc cagttccagg tgaaggcaac caaggaatcg ggaatgacgc
      361 ccatggactt gcgcacccta cttgattggt tcatcgcgta ccaattgcgt ggggtcgaag
      421 gggtcaccga gatcaacgcc cacggcggcg agatgaaaac gtacgaggtc gagcccgatc
      481 ccgacaaact ggcgaaccac cggctgtcga tgaccaatct ctttgacgca cttcggtcga
      541 ataacgccaa tgtggggggc ggctatctgg tacgcgaagg ggaagcgcgc tacatccgcg
      601 gtgtgagcca ggtgcgtact attgacgata tcgccgcgat tgtcatcgac gagcgcgacg
      661 gaattccggt gacggtcgct catgtggcta aagtgcgcgc cgcacccatg atccgcgctg
      721 gcatggcgac gcgcgacggc gagggagaga tcgtcacggg actggtcatg atgctgatcg
      781 gccagaacgg ccgcgacgtc gtcactcggg tgaaagcaga gatcgaaaat ttgaaaaaaa
      841 gcctgccccc gggcgtaacg atcgagccgc tctacgaccg tacccacctc atcaatcaaa
      901 ccctaaacac cgtactgcat aacctgacgg ccggtggcgt gctggtgatg gcgattctcc
      961 tggccttttt gggtaacctt cggggcggcc tgatcgtcgc gctggcgatt ccgctatcca
     1021 tgctgtttgc cgccaacgtg atgctggcga cgggaatctc ggccagcctg atgagcttgg
     1081 gcgcgattga ttttggcctc atcgtcgatt cgagcgtcat catgatcgag aactgcgtgc
     1141 ggaggctggc gcacgagggg gggaccaggc cgaaactcga catcgtgcgc gacgcggcca
     1201 tcgaggttcg taaacccacg atgttcggtg agctgatcat cgcgatcgtt tacctgccaa
     1261 tcctggcgct agaagggacc gagggcaaac tgtttcggcc catggcattg accgtcatct
     1321 ttgccctggc gggctcgctg ctcctgtcgc tcacgttgat gccggttttg gccgcaatgg
     1381 gcttgagctc gcggcctcag gagaaggaga tctggctcct gcgctggatc aaacacctct
     1441 atcaaccaat gctcgatacc ttcgtgcgcc atcccactgc cgcggtgttc ctggccctgg
     1501 gcttgatcgg agccagcgtg cccatcgcct tgaacctggg gggcgagttc atgccgcggc
     1561 tcaacgaggg tgacctcttg atcgaggcag tccggatccc ggcggcctct ctcgaagggg
     1621 ccgccctagc gtctactcag atcgaaaacc tgctcaagac gatccctgag gtgcggttgg
     1681 tctactgcaa gacgggccgg cccgagatcg ccaacgacgt catgggcgtg caccagaccg
     1741 acgtctggac catgctcaaa cctcaggaag aatggcgccc gggtttgact cgcgacgcat
     1801 tgatcgaaga aattgacaaa ctcttaaccg agcacgtccc cggcgttaaa ttcggtttca
     1861 gccagccgat cgagatgagg gtcaacgagc tggtagccgg agtcaagagc gacgtggcgg
     1921 tgcttattca tggcgcggat ctcaaagtgt tgcgccggct gtcgctcgag gtcgagcggg
     1981 tgctgagcca gatacccggc gcgcgcgaca tcaaaaaccc gacggcaggc cggttgccga
     2041 tgctacggat caaggtacgc cgtgatcagt tggcgcgcta cggcatcaag gcctccgacg
     2101 tgctcgactc ggtggccgcg ctcggaggca cgacggtcgg caccgtgttc gatgacgaca
     2161 agcggcaccc gttgcgcgtc cgccttcctg agtcgtggcg caacgacgcc gacaagatcg
     2221 gctcgatccg cgtcatcgat ccgcagggat ggccgattcc tctcaaagat ctcgccgaga
     2281 tcgcagagga ggaaggacct aacgaagtcg agcgtgagaa tgtgcagcac cgggcggtcg
     2341 ttggtgccaa cgtacgcggc cgcgatatcg ccagtttcgt cgccgaagcc caggccgcga
     2401 tcgatgaaca ggtcaagcgt ccgtatggct acgtgctgcg ctggggtgga caattcgaac
     2461 acctccagac cgcctcaaaa cggctgacga tcgtggtgcc tgtggcgatg ctcttgatct
     2521 ttctgctctt gtacagcaca tttcactcga tgcggctggc gttcctcatt tacacggcgg
     2581 tgccgatggc ggcgaccggc ggcattttct cgctgtttct ccggggcctg ccgttctcga
     2641 tctccgcagg tgtcggcttt atcgcccttt ttggagtggc cgtgctcaat ggtctcgtct
     2701 gggtcagcgc ggtcgagcat ctgcggcaag agggagtcga gccgcatgag gccgcgcgcg
     2761 aggcggccgt cgtccggctg cggccaatcc tgatgacagc actggtcgcc ggattgggct
     2821 ttatccccat ggcggttgcc acaacgcccg gagcagagat tcagcgcccc ttggccacgg
     2881 tagtcatcgg cggactgata acatccactc tattgacgac gctggtgctg ccggcggttt
     2941 acccctggtt tgcccagaaa cccaatgttc tcgaggctta ggttattgaa ttcgggcccg
     3001 ttggccaccg ccaaattctc cggaggaatt cggagaaatt aggtccgaac g
//
LOCUS       DATAQL010000890         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1312922, whole genome shotgun sequence.
ACCESSION   DATAQL010000890 DATAQL010000000
VERSION     DATAQL010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312922"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VHS97_15415"
     CDS             complement(<1..1167)
                     /locus_tag="VHS97_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX3449642.1"
                     /translation="MAGPARVDHKITLEEKSLRLLFDGGTLIAEGISANEEPDLPGVK
                     YDNRTRQFRAEAIWYRTIVEHIRKNKRPYTDGARAYEPAAWRLLVAKAPFPHQVEGLE
                     AWWNAGGRGVVVLPTGTGKTHLANMAIERAGRPTLVVTPTIDLMNQWYDELTLSFGVD
                     VGLLGGGYYDIQPLTVTTYDSAYLNMDRLGNRFGFIVFDECHHLPGPTYGLAATCAIA
                     PYRLGLTATPERADNAHTHLDQLIGPVVYRREITELRGDYLAEYRAMILYVNLSDPER
                     ERYEKAREVYRGFVQSTGINMSQPNAWAQFIFLAHRSPEGREAFQAYREQRELSLAAP
                     AKLNLLARLLERHNGDRVLIFTHDNATVYKIARQFLVPVITHQTKTKERREILLR"
     gene            complement(1379..2017)
                     /locus_tag="VHS97_15420"
     CDS             complement(1379..2017)
                     /locus_tag="VHS97_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX3449643.1"
                     /translation="MSAYRYIALFKPYDVVCQFSDDIGRLTLKDYVPIPGIYPVGRLD
                     RDSEGLLLLTDDGRLAHRLTDPRFEHPKTYLVQVERTPDHVALEELRRGVVLKDGRTR
                     PAQVELLAQPPALIDRSVPIRFRKNVPTAWLRLTIREGRNRQVRRMTAAVGHPTLRLV
                     RTAIGPITVGELAPGQWRELNAHELAALVRIREDRLDRRRRPDPRDRRREES"
     gene            2141..3229
                     /locus_tag="VHS97_15425"
     CDS             2141..3229
                     /locus_tag="VHS97_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX3449644.1"
                     /translation="MNDPPIPSPKGRGARLNPPNRFETTHHELELEQVEEDEEYLAAL
                     GRPKTEFLPDRSRSIIATNDSPDVGFEVSINPYRGCEHGCIYCYARPTHEFIGFSAGL
                     DFETKILVKHDAPELLRKALSAPGWTPRVLGLSGVTDAYQPVERRLMLTRRFLMVLAE
                     YRQAVTIITKNRLVIRDIDLLEELARYQAAGVFVSITSLDEALIGKLEPRTTRPEGRL
                     NAIAALSTAGIPTGVMVAPVIPGLTEHEIPSILKAAAQAGARSAGYTVVRLPLAVAGL
                     FENWLEQHFPERKDKVLDRIRATKGGRLNDSRFGVRMSGEGNAAEMIGQIFRTACRRA
                     GLNRAPWPVNNSAFRRPKPTSGQLTLFE"
BASE COUNT          670 a          984 c         1067 g          699 t
ORIGIN      
        1 ccgaagcagg atctcgcgcc gctctttggt cttggtctgg tgtgtgataa ccggcaccag
       61 aaactgtctg gcgatcttgt agacggtggc gttgtcgtga gtaaagatca acacgcggtc
      121 gccgttgtgg cgttcgagca gccgcgcgag caggttgagc ttggcgggag cggccagcga
      181 gagctcgcgc tgctcacgat aggcctgaaa ggcttcgcga ccttcggggg atcggtgcgc
      241 caggaaaatg aactgggccc atgcgttggg ctggctcata ttaatgcccg tgctttgaac
      301 gaagccgcga taaacctcgc gcgctttttc gtagcgttcg cgctcgggat cgctcaagtt
      361 cacataaagg atcatcgccc gatactcggc cagataatcc ccgcggagct cggtgatctc
      421 gcggcggtac acgactggac cgatcagctg atctagatgc gtgtgtgcgt tgtccgcacg
      481 ttcgggggtt gcggtcagtc ccagccgata cggggcgatg gcgcaggtcg ccgccaatcc
      541 gtaggtcggg ccgggtaagt ggtggcattc gtcgaacacg ataaacccaa atcggttgcc
      601 caggcggtcc atgttcaagt aggccgagtc gtacgtcgtc acggtcagtg gctgtatgtc
      661 gtaataaccg ccccccaaaa ggccgacgtc aacgccaaag ctcagggtga gctcgtcgta
      721 ccactggttc ataaggtcga tggtgggggt gacgaccagc gtgggccggc cagcccgctc
      781 gatcgccata ttggcgaggt gggtcttgcc agttccggtc ggcaaaacga cgacgccccg
      841 cccgcccgcg ttccaccaag cttccaggcc ctcgacctgg tgcggaaagg gggctttggc
      901 caccagcagc cgccacgcgg cgggctcgta ggcacgtgct ccatccgtat aggggcgctt
      961 gttctttcgg atgtgctcga cgatggtccg ataccagatc gcctcggcgc ggaattggcg
     1021 tgtgcgattg tcgtacttca cgcccggcaa atccggctct tcgttcgcgg agattccctc
     1081 ggcgatcaaa gtgcccccgt cgaatagtag acggagagac ttttcctcga gcgtgatctt
     1141 gtgatcgact cgagctgggc cggccatcgt cgagctccgt cagggaacca caccgcaaat
     1201 cccatcgggt cgaattggcc gctcgccaga agccgccggt gcaattcgag cggattcaaa
     1261 ccctttgtac gcgatccatc gggcgggcaa cacgtcgacc caacactcta ttcagtgtac
     1321 tcccgctcga gggccagcgt gagatgaaac gcgatttgaa acgaaaacct gtcggatctt
     1381 acgattcctc tcgcctccga tcgcgcggat caggtcgccg gcgccgatca agccgatctt
     1441 cgcggattct cactaatgcg gcgagttcgt gggcgttgag ctcgcgccac tggcccgggg
     1501 ccaattcgcc aaccgtaatc ggaccgatcg cggtgcggac cagccgcagg gtgggatggc
     1561 cgacggcagc ggtcatccgg cggacctggc ggttacgccc ctcgcggatc gtcagccgca
     1621 gccaggccgt cggcacgttt ttgcggaatc ggatcggcac cgatcgatca atgagcgcgg
     1681 ggggctgggc gagcagctca acctgggccg gtcgagtccg accgtctttt aagacgacgc
     1741 cacggcgcag ctcctcgagt gcaacgtgat cgggtgtccg ctcgacttga accaaatacg
     1801 ttttgggatg ctcgaaccgc ggatcggtga ggcgatgcgc cagtcgtcca tcgtcggtca
     1861 agagcaagag accctcgctg tcgcgatcga gccgtccgac cggataaatt cccgggatcg
     1921 gtacgtaatc cttgagagtg agccggccaa tgtcgtcact gaactggcat acgacatcat
     1981 agggcttgaa aagggcgatg tagcggtatg cgctcaactg gttgtctcgc gggcattatc
     2041 tggactcgga tattgttcca atgtacagta taattcgggt gtcgatttgc tggagagcaa
     2101 tgttgccaca atgaggttgg gccaaagagg tgattatcac atgaatgatc caccaattcc
     2161 atcgccgaag ggacgaggag cccgactcaa ccctccgaac cgctttgaaa cgacgcatca
     2221 cgagctcgag ctggaacaag tggaggaaga cgaggaatat cttgccgcat tggggagacc
     2281 caagacagag ttcttgcccg atcggtcccg ctcaatcatc gccacgaacg atagcccgga
     2341 cgttggcttc gaggtgagca ttaatcccta ccgcgggtgc gagcacggct gtatttattg
     2401 ttatgccaga ccaactcatg aatttatcgg attttccgcc ggtcttgact tcgagaccaa
     2461 gattctggtc aagcatgatg cgcccgaatt gctaaggaaa gccctgtcgg cgccaggatg
     2521 gacgccacga gtgctgggct tgagtggtgt tactgatgcc tatcagcccg tggagcggcg
     2581 gttgatgctt accaggcgct ttctcatggt cctagccgag tatcgtcagg cggttacgat
     2641 catcaccaag aatcgactgg tcatacgcga tatcgatctc ttagaagagc tggcacggta
     2701 tcaagcggca ggcgtctttg tgtcgatcac atcgcttgat gaagcgctga tcggcaagct
     2761 ggagccgcgc acgactcggc ccgaggggcg gctgaacgca atcgccgccc tgagtactgc
     2821 ggggataccg acgggcgtga tggtcgcgcc ggtgattccg ggactgaccg aacacgaaat
     2881 cccctcgatc ttgaaggccg ccgcgcaagc cggggcgcgg tcggccggtt acaccgtcgt
     2941 gcggctgccg ctggccgttg cgggcctctt cgagaactgg ctggagcaac atttcccgga
     3001 gcggaaggat aaggtgctcg accgaattcg cgccacgaag ggtggaaggc tcaacgattc
     3061 gcggtttggc gtgcggatgt cgggtgaagg aaatgcggcc gagatgatcg gccaaatatt
     3121 ccgcaccgcg tgtcgtcggg cggggttgaa ccgcgcgccg tggcccgtca ataactcggc
     3181 tttccgccga ccaaaaccga cgagcgggca gctcactctc ttcgaataac gccgccagga
     3241 ctttgtcacc ggatggtaga tcgcccccaa tcacttgtcc ggacgggctt tgcggctagt
     3301 tctctctcag gccaaattgc ggttggcccg attgcggtat tttattaaag ttcccactcc
     3361 tgagtttcga cacggatgtc gggcttgggt tacttcgttg cgtccacaca tccgtccgat
//
LOCUS       DATAQL010000891         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2384281, whole genome shotgun sequence.
ACCESSION   DATAQL010000891 DATAQL010000000
VERSION     DATAQL010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384281"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VHS97_15430"
     CDS             complement(<1..1176)
                     /locus_tag="VHS97_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3449645.1"
                     /translation="MSANSHQTASREGSTSTSSNGPAASGGPSLPTTIGQYLVERLAA
                     LGVEHVFGIPGDYILQLYKLIDESPIKLVGVTREDNAGFAADAYARIHGLGCICVTYC
                     VGGLSTCNSIAGAYAEKSPVIVLAGSPGMSERARNPLLHHKVKGFETQFEVFQKITVA
                     SAVLDRPETAFSEIDRVLEAAVRYKRPVYLELPRDQVGARQVTPHRPPEGLPKSDPEA
                     LREAIDEAAALLTAARRPVILADVEIHRFGLQNELLTLAESTGMPIATTLLGKSVISE
                     AHSLFGGIYEGAMGQPEVTELVESADCLLMLGCFLTDINLGIWTAKLDPSRCIDATSE
                     DLRIKHHHYEDVRLDDFIGGLIERKLKVAKTPVPSRIQPFSKLPAAQPDERMTSARLF
                     "
     gene            complement(1187..1570)
                     /locus_tag="VHS97_15435"
     CDS             complement(1187..1570)
                     /locus_tag="VHS97_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX3449646.1"
                     /translation="MSSRLFAYGTLMPADAAAVARQGWTADAVRGRLFDLGPYPGLID
                     LDASGAGWVGGYVRSVESLELEAFDRWEDVDLGAYRRVETTTRDGVRVWVYVYARPLP
                     PEARGPLPRWRNHLLAQESNRLSFG"
BASE COUNT          452 a          686 c          671 g          432 t
ORIGIN      
        1 aaagagccgg gccgaggtca ttcgctcgtc gggctgggcc gcgggcagct tggagaatgg
       61 ttggattcga ctcggtacgg gggttttcgc aaccttgagc ttgcgctcga tcaagccgcc
      121 gatgaagtcg tcgagcctca cgtcttcata gtgatggtgc ttgatccgca gatcttcgct
      181 ggtggcgtcg atgcaccgcg acggatcgag tttggccgtc cagatcccca gattaatgtc
      241 ggtcaagaag cagccgagca tcaagaggca gtcggcagac tcgacgagtt cggtcacctc
      301 gggctggccc atcgccccct cgtaaatgcc gccgaaaagc gagtgggcct cggagatcac
      361 gctcttgccc aggagagtcg tcgcgatcgg catgcccgtc gattcggcca gggtcaagag
      421 ctcgttttga aggccgaatc gatggatctc gacatcggcg aggatcaccg gccgccgcgc
      481 ggccgtcaag agcgcggccg cctcgtcgat cgcctcgcgc aaagcctcgg ggtcgctctt
      541 gggcagccct tccggcggcc gatgcggcgt gacttgccgc gctcccacct ggtcgcgcgg
      601 cagctcgaga taaacggggc gtttgtagcg gactgccgct tccagcaccc gatcaatctc
      661 gctaaatgca gtctcgggcc ggtccagaac ggccgaggcg accgtgattt tctggaagac
      721 ctcaaactgg gtctcgaatc ccttgacctt atggtgcaag agcggatttc gggcacgttc
      781 cgacattcct ggtgaccccg ccagcacgat caccggcgac ttttcagcat aggccccggc
      841 aatgctattg caagtcgaga gtccccccac acagtacgtg acgcagatgc agcccaaccc
      901 gtgaatccgc gcataggcgt ccgcggcaaa cccagcgttg tcctcccgag tcacgccgac
      961 cagcttgatc ggactttcgt cgatcagctt gtaaagctgg aggatatagt cgccgggaat
     1021 gccaaaaacg tgctcgaccc ccagagccgc caggcgctcg accaggtact ggccgatggt
     1081 cgtcggcagg gacggtcccc ctgatgccgc cggcccgttg ctagacgtag acgtcgatcc
     1141 ttcgcgggag gcggtctggt gcgagttggc tgacatcgca atctccttat ccgaatgaaa
     1201 gacggttcga ttcttgggca agcaagtgat ttcgccagcg gggcaacggc cctcttgcct
     1261 ccggcggcaa cggccgggca tacacataaa cccagacgcg aaccccgtcc cgcgtcgtcg
     1321 tttcgacccg ccggtacgcg ccaaggtcga catcctccca cctgtcgaag gcctccagct
     1381 caagggactc caccgatcgg acatagcccc cgacccagcc ggcgccggaa gcatcaaggt
     1441 cgatcaagcc agggtaaggg ccaaggtcga aaagccgccc gcgcaccgcg tcggccgtcc
     1501 agccctggcg tgctaccgca gccgcgtccg cgggcatcaa agtgccatag gcaaagagcc
     1561 gtgacgacat cgcggtttac tctcaggcgc gaactcggga tcggccgatc aaacgctact
     1621 attattgtat tcgatactgt gggaacaatc ggaattcccc cttacggaat tccggatgaa
     1681 caatattcgc ccgcgtttac atgccgttcg aacaaattgc tttctaggca ccctggcctg
     1741 aaccgattcg cctcatcggc agaattgata aaatctggac aaaacaccga tgggttcgtt
     1801 tggtcggcgc gatttccggt ttgaaataag ctttagtgac tctccaacat agagttgcga
     1861 ccgacgcaca cttggggaaa aatggcttcg ttcggcgcat tcactcggat gatccgcccg
     1921 aaattgggtt cgatcggcgc gatttatgtg cccgccgtat tcatgtcatg ctcgagaacc
     1981 cgcgtgcgtg tgcgttgatg agttcaccga ccggatgatc ccgcatcggt atgatcggca
     2041 ggggacggaa acgacttgca taaacgatca cggatttgat cgaatcctgg agttggaacg
     2101 accgtgagag tggtcacgac aattgacaag gggccgatcg agataatcga tggtcggatg
     2161 gtccacgacg gcttcgcgtc gtacgaccgg ttccgggcgg cgacccacca gcagtcgctg
     2221 ggccacgtcc tgcggcgcgt g
//
LOCUS       DATAQL010000892         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4214624, whole genome shotgun sequence.
ACCESSION   DATAQL010000892 DATAQL010000000
VERSION     DATAQL010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4214624"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VHS97_15440"
     CDS             <1..255
                     /locus_tag="VHS97_15440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX3449647.1"
                     /translation="KAAIQYEGKLENGQVFDSSKGRSPYPFGQSPAIKGWEEAIPGMK
                     LGEIRKLTIPPAMAYGEQGSPPKIPPNATLIFEVELVEIK"
     gene            459..1316
                     /locus_tag="VHS97_15445"
     CDS             459..1316
                     /locus_tag="VHS97_15445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449648.1"
                     /translation="MTNVSGPPKLSGMKYRVVNTLGAGAGSTILLIADKTAGGKRYAL
                     KTVRKQEPEDEIYVQQALTEFEAAKKLNHPAIAKVFDCRMKKAWFKVKGVDLLLEYVD
                     GKTLDEIEAPELGQIVLIFCQIASALTHMHRRGVFHGDLKPSNIMLTKTGQVKLIDFG
                     TAWVRGQDKNRVQGTPNFIAPEQANDKVVNEKTDIYNMGATMYRMFTGRFAQQGIPKI
                     GDERKLVPPVKINPRIPGSLNELIVASLSIDPSRRPAGMFEIRDQLSAVAKQMGLAEV
                     DLRGADEED"
     gene            1552..>3218
                     /locus_tag="VHS97_15450"
     CDS             1552..>3218
                     /locus_tag="VHS97_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEX3449649.1"
                     /translation="MQYNPANPLIVQGDRTILLEVDNPLHSEARDAIAPFAELEKSPE
                     HIHTYRLTPLSLWNAAAAGMSADAMIVALERFSKFPLPSNVPADIRELVGRYGRVRLE
                     RIGGQLRLITQDRPLLEELARQRGVRDYLGERFDDTSFKIDDANRGVVKQALIAVGYP
                     AEDMAGYTPGAALPVTMRNQTRAGLPFEVRDYQQGAVAAFYAGGDVRGGSGVIVLPCG
                     AGKTIVGLAAMGVLKTETLILTTSTTAVEQWKREILDKTDVDPELIGTYTGDSKTLAP
                     ITLATYQIVTYRPKKDGDFPHFHLFDQRDWGLIIYDEVHLLPAPVFRITADIQARRRL
                     GLTATLVREDNREEDVFSLIGPKKYDVPWRVLESKGWIAEARCHEVRLGLPAPLRMEY
                     ALAEWREKFRLASENPIKDELVAMLLERFDGPDDRVLVIGQYLKQLRRIAERFDIPLI
                     TGQTPNSEREELYGNFRRGVLRRLILSKVGNFAIDLPDANVMVQVSGTFGSRQEEAQR
                     LGRILRPKEGEVPARFYTLVSRDTREMDFAHHRQLFLTEQGYTYEIL"
BASE COUNT          688 a          946 c          978 g          606 t
ORIGIN      
        1 aaggcagcga ttcaatacga gggtaagctg gaaaacgggc aggtcttcga ttcgagcaag
       61 ggcaggtccc cttacccatt tggccagagc cctgccatta agggctggga agaggccatc
      121 ccggggatga agcttggcga aatccgcaag ctgaccattc ctccggcaat ggcttacggc
      181 gagcagggct ccccgcccaa gattcccccc aacgcgacgt tgatcttcga agtggagctt
      241 gttgagatca agtgaacgcg aagccccaac tggactacgg ctttctcgtg cccgacgcag
      301 gggattgctc cgccagaggt cttgagataa cctcgtcgag tcgaaaagcc cgcgcggcag
      361 ccctccggga ccagcgggtt tcttgattct cactcgatcg aaagtgacgg cgcgatcgcg
      421 cggactaacg gccagcagca gcgtgaatga ggaacggtat gacgaatgtg tcgggtccgc
      481 ccaagttgtc cggcatgaaa tatcgcgtgg tcaacacgct cggtgccggc gcgggaagca
      541 caattctgtt gatcgcagac aagacggctg gtggcaagcg atacgccctg aagacggttc
      601 gtaagcaaga gcccgaagac gagatctacg tgcagcaggc actgaccgag ttcgaggctg
      661 ccaaaaagct caaccacccg gcaattgcca aggtctttga ttgccggatg aagaaggcgt
      721 ggttcaaggt caagggggtc gatctcttgc tcgagtatgt cgacggcaag acactcgacg
      781 agatcgaggc gcccgagctc gggcagattg tgctgatctt ctgccagatt gcttcggccc
      841 tgactcacat gcaccgcaga ggggtttttc acggcgactt gaaaccatcg aacatcatgc
      901 tgaccaagac cggccaggtg aagctcatcg atttcggcac agcctgggtg cgcgggcagg
      961 acaagaaccg cgtccaagga acgccgaact ttatcgcccc cgaacaggct aatgacaagg
     1021 tcgtcaacga gaagaccgac atttacaata tgggcgcgac gatgtaccgc atgtttacgg
     1081 ggcggttcgc tcagcagggt atacccaaga tcggcgacga gcgtaagctt gtccctccag
     1141 tcaagatcaa tccgcgcatt cccggatccc ttaatgagct gatcgtcgcc agtctcagca
     1201 ttgacccgtc gagacggccc gccgggatgt tcgagatccg cgatcagttg agcgctgtcg
     1261 ccaagcaaat gggattggcc gaagtcgact tgaggggcgc cgacgaagaa gactgaggga
     1321 cgggggttac atatcgaact ttcggaaatt tggccgggtt cgcatcgatg gggccaattt
     1381 cccgtggtgg accagcccga cgggcgatca ggaacgcgcc atctggacca gccaaccttg
     1441 cgagaaaagt cgtccacata tacgatggaa cgttcgcgct tggggggcgt gacgacgtcg
     1501 cgtttgtgaa gcgcacaggc cccgagtcag tcccgatccg gcaaggagcc catgcagtac
     1561 aatcccgcca atccactgat cgtgcagggt gaccgtacga ttttgcttga ggttgacaat
     1621 ccgctgcatt ccgaagcccg cgatgcgatc gcgccttttg ccgagctaga aaagagcccc
     1681 gagcatattc acacctatcg gttgacgccg ctttcgctct ggaacgcggc agcggccggt
     1741 atgtcggccg acgcgatgat cgtggctctt gagcgtttct caaagtttcc tcttccttcc
     1801 aatgtcccgg ccgacatccg tgagctggta ggccgatacg gtcgagtgcg gttggaacgc
     1861 attggcgggc aactccggct gatcacccag gatcgccccc tgctcgaaga actggcccgg
     1921 cagcggggcg tgcgcgacta tttgggcgag cgattcgacg acaccagttt caagatcgac
     1981 gacgccaatc gcggggtagt aaagcaggca ctgatcgcgg tcggctaccc ggccgaagat
     2041 atggccgggt atacacccgg ggcggccctt ccggtcacga tgcgcaacca gacccgtgcc
     2101 ggtctccctt tcgaggtccg tgattatcag caaggggcag tcgcggcctt ttacgccggc
     2161 ggcgacgttc gcggcggttc cggcgtcatc gtcttaccat gtggcgcagg caaaacgatc
     2221 gtcggcctgg ccgcgatggg agtgctcaag accgagacgc tcattctcac cacgagcacg
     2281 acggccgttg agcagtggaa gcgcgagatt ctcgacaaga ccgacgtcga tcccgagctg
     2341 attggaactt acaccggcga ttccaaaacg ctggcgccga tcacgctggc cacctaccag
     2401 atcgtgacgt acaggcccaa gaaagacggt gacttccctc actttcatct attcgaccag
     2461 cgcgactggg gattgatcat ttatgacgag gttcacttgc tgcccgcgcc cgtgtttcgg
     2521 atcacggccg acatccaggc ccgccgccgc ctcgggctga ccgcgaccct ggttcgcgag
     2581 gacaatcgtg aggaggacgt cttcagcctg atcgggccca aaaagtacga tgtaccctgg
     2641 cgcgtgctcg agtcgaaagg atggatcgcc gaggcgcggt gccacgaggt tcgactgggc
     2701 ttgcccgccc ctctgcggat ggagtatgca ctggccgagt ggcgggaaaa gtttcgcctg
     2761 gctagcgaaa atcccatcaa ggacgagctc gtggccatgt tgctcgagcg gttcgacggt
     2821 cccgacgatc gggtgctggt gattggtcag tatctcaagc agctgcgccg gatcgccgaa
     2881 cgatttgaca ttccgcttat cacgggccag acacccaaca gtgaacgcga agagctctac
     2941 ggcaattttc gccgcggtgt gctccgccgg ttgattctct cgaaggtcgg taacttcgcg
     3001 atcgacctgc ccgacgccaa cgtgatggtc caggtttcag gaaccttcgg cagccggcaa
     3061 gaagaagcac agcgcctggg ccgcatcctc cggcccaagg agggagaagt tcccgcacgt
     3121 ttctacactt tggtgagccg tgacacccgc gaaatggatt tcgcccacca ccgtcagctc
     3181 ttcttgaccg agcagggata cacctacgag atcctcga
//
LOCUS       DATAQL010000893         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3449589, whole genome shotgun sequence.
ACCESSION   DATAQL010000893 DATAQL010000000
VERSION     DATAQL010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449589"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1563)
                     /gene="bamD"
                     /locus_tag="VHS97_15455"
     CDS             complement(166..1563)
                     /gene="bamD"
                     /locus_tag="VHS97_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX3449650.1"
                     /translation="MPEYVSLLLVWCASASGLRGPRSSRRRVGHLLRLALTLLPVLFT
                     GCQSFSNPISQWRAAYDGGLFKGVSKEEMADASSSMDSNNLFERWITPRRSPMGKSDG
                     SNPSTLVLGSDGWRPMTKTPKDPTAEAELQSALTLFQQGKFDEAEKAYAKIAKDRKST
                     PWGETAQYYLAETQYQSGKFVDAHDSFEKLHADYPVTDYLDKLVSREYAIAQLWMQQD
                     DPNAPKDKLLPWYGRFDGRLPIIDTQGYALKALEHVRHNDPTGPLADDAALQIAEYYV
                     KHRDYESASLYYDQFIAEYPKSPFLQKVQHAAIDTRLRAYLGPEYDASGLEKARAMVQ
                     KTMKTFPERQASYEGLYHTLDVINNAEAEKTFLTGTYYKRVGKIPAAEFYLGKIPQRW
                     PNSPWAVKAKGELASLAKLPRTPSKPSKIMIPPGSTDPFGTGMGGMGGMGMGGMGMGG
                     MGMGGMGMGGMGGMM"
     gene            complement(1601..2347)
                     /gene="recO"
                     /locus_tag="VHS97_15460"
     CDS             complement(1601..2347)
                     /gene="recO"
                     /locus_tag="VHS97_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX3449651.1"
                     /translation="MPANRTLALVVRTVEVFETSLVATLFTRELGKVAVLAKGARRLK
                     SPSQGGLDLLGVSDIVLFPKASEALDLLAEAVPVERFACLRRDLRALYAGYYIAELLT
                     DLTDLHDPHPRLFDAARVTLRHLVEADLRQQRIIRFELACLRELGLMPTLELCAACGA
                     LVERQTAMVSFGLAAGGVVCSACRPGLTHVTDLSVNDLEAIRVLASPGSAWRQLDARN
                     GCLAAARETVGAVVSHVLGHRPRLRPLLGV"
     gene            2804..>3768
                     /locus_tag="VHS97_15465"
     CDS             2804..>3768
                     /locus_tag="VHS97_15465"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449652.1"
                     /translation="MRRGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRAQCTNNL
                     KQIGLAMHNYHQTNNVFPQGHSQSAIQLPYTAYAGWGEWSAQAMMLNYMEQTPIYNSI
                     NFSLVGAYSYGAFANVSVFNLPIASFMCPSDNNVDKGGPPNNTPSGPSGWGGNSTYPP
                     NINSYRGSVGTTTARYSWPPNTGYACCTPDPFNFTGNGTPAQKPASTGMFAYWTSYGI
                     QDATDGSSNTILFAESLVGDAGGTQSNSATNRNNSVTNVTAIGTNVSVFDASSLNYQT
                     VIVPAINQCAQAMRAGTGVVTDNGNRWAWGAVGISLFNTVVPPNGA"
BASE COUNT          789 a         1135 c         1097 g          747 t
ORIGIN      
        1 ttcgcggctg tcaattcgtc gcgagcatcg gaaaagtaca ttcttcatgg caccgaggct
       61 cccgggagaa caggacgcag attccagacc ggcaagttac gcaagctcgt ttggatccat
      121 tgatcacgtg atactgtcat atgaatataa ctgccggcgg tagactcaca tcataccacc
      181 catgccgccc atgcccatgc cacccatacc catgccaccc atgcccatgc cgcccatacc
      241 catgccaccc atgccgccca taccggtgcc aaacggatcc gtcgagcctg gggggatcat
      301 gatcttgcta ggcttcgacg gagttcgggg gagcttggca agagacgcca gctcaccctt
      361 ggccttgacg gcccatggac tgttgggcca gcgctgaggg atcttgccca gataaaactc
      421 ggcggccggg atcttgccga cgcgtttgta ataggtgcca gtcaagaagg ttttctcagc
      481 ctccgcgttg ttgatgacgt cgagcgtgtg gtagagcccc tcataactgg cctgacgttc
      541 ggggaacgtc ttcatggtct tttgcaccat ggcgcgtgcc ttctctagac ccgacgcgtc
      601 gtattcgggc ccaaggtacg cccggagccg agtgtcgatc gccgcatgct ggaccttttg
      661 aaggaaagga ctcttggggt actcggcgat gaactgatca tagtaaagcg aggcggattc
      721 gtagtcgcgg tgtttcacgt agtattcggc gatctggagc gcggcgtcat cggccaacgg
      781 tccggtagga tcattgtgcc gaacatgctc gagggccttg agcgcgtacc cttgcgtgtc
      841 gataatcggc aaacggccgt caaagcgacc gtaccatggg agaagtttgt ccttgggcgc
      901 gttggggtcg tcttgttgca tccagagctg ggcgatggcg tattcgcggc tgacgagctt
      961 gtcgaggtag tccgtgacgg gatagtcggc gtggagcttt tcaaagctgt cgtgggcgtc
     1021 gacaaacttg ccgctttggt actgcgtctc ggccaggtaa tactgggcgg tctcgcccca
     1081 cggcgtagac ttgcggtcct tggcgatctt ggcgtacgct ttttccgcct cgtcgaattt
     1141 gccctgctga aagagggtga gggccgactg caactcggcc tcggccgtgg ggtcctttgg
     1201 cgtcttggtc atcggccgcc agccgtcgga tcccaggacc agggtcgatg gattcgagcc
     1261 atccgacttg cccatgggac tgcgccgcgg cgtgatccag cgctcaaaga ggttgttgga
     1321 atccatcgat gagctggcat ccgccatctc ttctttcgag acccccttga acaggccgcc
     1381 gtcgtaagcc gcccgccact gcgaaattgg gttgctgaaa ctttgacagc ccgtgaataa
     1441 gaccggcaag agcgtcagcg ccagccgcag aagatgcccg acacggcgtc gcgacgagcg
     1501 gggcccgcgc aaacccgacg cggaggcgca ccagaccagt agaagactca cgtactcagg
     1561 cattgcacga acgtttcggt ggtcaacccg acccatcggg tcacactccc agtaaaggcc
     1621 ggagccgagg acgatggccc aagacatgac tgacgactgc cccgaccgtc tctcgagccg
     1681 ctgccagaca cccatttcgg gcatcgagct gccgccacgc gctgcccggg ctggcgagaa
     1741 cgcgaatcgc ctcaagatcg tttaccgata ggtcggtcac gtgcgtcaaa ccgggccggc
     1801 aggccgaaca aacgacccca ccggcagcga ggccaaacga aaccatcgcc gtctgtcgct
     1861 cgaccaaggc gccgcaggcg gcgcacagtt caagcgtcgg catcaatccc agctcgcgca
     1921 ggcaagccaa ctcaaaccga atgatccttt gttgccgcaa gtctgcttcg accagatgcc
     1981 gcaaggtgac tctcgccgcg tcgaacaacc ggggatgcgg atcgtgcagg tcagtcagat
     2041 cggtcaacag ttcggcgata tagtagccgg catacagggc acgaagatcg cgacgaagac
     2101 atgcgaagcg ttcgacaggc acggcctcgg ccaacaagtc gagggcctcg gacgctttgg
     2161 gaaagagcac gatatcggaa acgcccaaca ggtcaagtcc accctgcgac ggtgacttga
     2221 gccgccgtgc acccttggcc agtacggcaa ctttccccaa ttcccgggtg aataaagttg
     2281 ccaccaggct ggtctcgaat acctcgaccg tacggacaac cagagcgaga gtgcgattgg
     2341 cgggcaactc ttagtaatct ccctatcgcc agctgggccg actaagcgcc actattgacc
     2401 gcgaccccgc ggcggactaa actaatttgt tagcattaaa tccttcggac aagacgcaaa
     2461 gcgaatcgtg ctcgcttcga gtgctagagc ggggaaaatg ggctgtgttg ggagctcttg
     2521 ttcaattttc accggagcga agggcttttt tgataatcgc gcgaaaaagg cctcaatgaa
     2581 ggtagtcggc tcgggagacc tatcagcctc ccctgctgcg attattcgat gattcctccg
     2641 ggtccgatag catcctcgat atccgctccg taagaaccct gccacttcaa tcttgcctgc
     2701 cagggcgcag cgcgagatcc cgagacctag cacatttgtt cgagccttct tgagtcgtgc
     2761 gaccgtttcc tcatttccga ttggagatcc tatgagtgcg agaatgcggc gaggattcac
     2821 gttgatcgag ctactggtcg tcattgcgat tatcgcggtg ctgatcgcgc ttttgctgcc
     2881 ggcggtacaa gcggcccgcg aggcagcccg gcgggcgcag tgcaccaaca atctcaagca
     2941 gattggtttg gctatgcaca attatcacca aaccaacaat gttttcccac aggggcactc
     3001 gcagtcggcc atccagttgc cttacaccgc gtatgcgggc tggggcgaat ggagcgccca
     3061 ggcgatgatg cttaactata tggagcagac ccccatatat aactccatca atttttctct
     3121 cgtcggggcc tatagctacg gggctttcgc gaacgtgtcg gttttcaatt tgcccattgc
     3181 atcgttcatg tgcccatcgg ataacaacgt ggacaaggga ggcccgccga acaacactcc
     3241 ctcgggaccc tcgggttggg ggggaaacag cacgtatccc ccgaatatca acagctaccg
     3301 cggctctgtc ggcacgacta ccgcaaggta cagctggcct ccgaacaccg gatatgcttg
     3361 ctgcacgcct gacccgttca atttcacggg taacggcaca ccggctcaaa agccggcctc
     3421 gaccggaatg ttcgcttact ggaccagtta cggcatccag gacgcgaccg acggcagctc
     3481 gaacacgatc ctgttcgccg aatcgctggt gggggacgcc ggaggcaccc agtcgaatag
     3541 tgctacgaat cggaacaatt cggtcacgaa cgtcaccgcc atcgggacga acgtcagcgt
     3601 cttcgacgcc agttcactca attaccaaac cgtcatcgtc cccgcgatca accagtgcgc
     3661 ccaggcgatg agggcgggca ccggtgtcgt cacggataac ggcaaccgct gggcctgggg
     3721 cgctgtggga atcagcttgt tcaacacggt ggtcccgccc aatggtgc
//
LOCUS       DATAQL010000894         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2839577, whole genome shotgun sequence.
ACCESSION   DATAQL010000894 DATAQL010000000
VERSION     DATAQL010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839577"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..812
                     /locus_tag="VHS97_15470"
     CDS             69..812
                     /locus_tag="VHS97_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449653.1"
                     /translation="MNVRGTKVTSLLFEHISRMTQLRFLDVGYSHVNDDLFEALENLE
                     HLEHFSFGGNKMSGAALPFLKALPALKVLDVSGQQRSDSGLWSVTMTDLCVGQIAQLP
                     QLEALDLGATMVSDRGLAELAHLKKLHTLDLRETRVTSKGIAALSGLPELRHLNLGRA
                     KGIDNATVLALLPMAALETLELTETNITAQGLERLSTKKGLRRLYIGGVNVKPEDVAA
                     ASKALPDCLVSWWPKPKIEYPEPPRRGGQ"
     gene            complement(895..2205)
                     /locus_tag="VHS97_15475"
     CDS             complement(895..2205)
                     /locus_tag="VHS97_15475"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3449654.1"
                     /translation="MTPRKTLSRRTFLRGVGLSGAAIHVGLPAFEAMFNASGTAYAAE
                     AGVSAQTIETRFVLWFNGNGIIEKYWIPREDGAQYEMTPCLQPLARYRQDIHVLSGVD
                     NPAARLPGPGNGHHNSMSGLVSGEMFSGRGAGGPSIDQVIAERIGNNSRFRSLQIGVC
                     QESFGESIQRNMSWAGRDRPLPPEMLPHRLFDRLFGAKEQYWIERKKSILDAVSDEAQ
                     ALKKALGHGDQQRLEEYLTSVRSLERSVASLPPEYARVVEQPPAGGDLKDWPLIAKLQ
                     TDLLVHALASRQTRVASYMLTKCQGLSRFPWLGHTHERHHEYTHGRVDSPEGQRIMRD
                     ICRWHAEEFAYFLAKLKSIPEGAGTLLDHTCALYIHEHAEANVHKNAGLAMIVAGHAG
                     QMKMGLHTRLTGTVGDLYQTLADEVVGARIGRFPTGEKKLSEIV"
     gene            complement(2209..>2885)
                     /locus_tag="VHS97_15480"
     CDS             complement(2209..>2885)
                     /locus_tag="VHS97_15480"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HEX3449655.1"
                     /translation="EFAKVAYPAGSGRAGVLGHGAFLLLTSKPAETSPTARGLFVRNQ
                     FLAQEIPPPPAGVNAVLATISENAPLTNRQRLAVHLNSESCASCHRMIDPIGLAFEQY
                     NAIGVFQKKMVLQFPGPGDGQTRGRTPMIKELDVDTSGFIQGMEDSEFSTPRELGRLL
                     AESKTCQKAVVKQLFRYAFGRQETASDQPTIDALVERFCNSGFRFRELIVAVVTSELF
                     LQKGSG"
BASE COUNT          575 a          914 c          845 g          551 t
ORIGIN      
        1 tgtactacgc ggagtctgta tcggacctgg ggatcgctca cctcaaacac tggcagaacc
       61 tcgagcattt gaacgtgcgc ggcacgaagg tcaccagctt gctcttcgag cacatctcga
      121 gaatgacgca gctccggttt ctggatgttg gctacagcca tgtcaacgac gaccttttcg
      181 aggctcttga gaaccttgaa catctcgaac acttctcatt cggcggcaat aaaatgagcg
      241 gcgccgccct gccgtttctc aaggcgctgc ccgcgctcaa agtattggat gtgtctggcc
      301 agcagcggtc cgactctggt ctctggagcg tgacgatgac cgatctctgc gtcgggcaga
      361 tcgcgcaact accacaactg gaagcgctcg acctgggcgc aacaatggtt tccgatcggg
      421 gccttgccga gctggcccac ttgaagaagc tgcacacgct cgacctgcgg gaaacacgag
      481 tgaccagcaa ggggatcgca gctctcagcg gcctgccaga actgcggcat ctcaaccttg
      541 ggagggccaa aggcattgac aacgcgaccg tcctcgcgtt gctgccgatg gcggccctgg
      601 aaacccttga attaaccgaa accaacatca ccgcccaagg tctggagcga ctttccacaa
      661 aaaagggcct gagacggctc tacatcggcg gcgtcaacgt caagccggag gacgtagcgg
      721 cagcgtccaa ggccttgccg gattgtctcg tgagttggtg gcctaagccg aagattgagt
      781 accccgaacc cccgcgacgt ggtggccagt gaaaaaatgc tggtcctcag tcatcgcgag
      841 ggccttcgac aatcgcccca tcgcgcccta catacatcac acctgggcaa aagctcacac
      901 gatttcggac agcttcttct cgcccgtagg gaaacgaccg attcgcgcac caaccacttc
      961 gtctgcgagg gtctgatata gatcccccac agttccggtc aggcgtgtgt gcaaccccat
     1021 tttcatctgg ccagcgtggc cggcgacgat catcgccagc ccggcgttct tgtgtacatt
     1081 ggcctcggca tgctcgtgga tgtaaagcgc acacgtgtga tcgagcaaag tgcccgcgcc
     1141 ctccgggatc gacttgagct tggcgaggaa gtaggcaaac tcctcggcgt gccagcggca
     1201 gatatcccgc atgatgcgct gaccttcggg tgagtccact cggccgtgtg tgtactcatg
     1261 gtgccgctcg tgagtatggc ccagccaggg aaagcgcgaa agaccctggc acttggtcaa
     1321 catataggag gccacccgag tctggcgcga agccagcgcg tgcaccagca ggtcagtctg
     1381 aagctttgcg atgagcggcc agtccttgag atcgccgcct gccggcggct gttcgacgac
     1441 ccgcgcgtat tccggcggca ggctcgcgac cgaccgttcg aggctgcgca ccgaggtgag
     1501 atactcttcg agacgctgtt gatcgccgtg cccgagcgcc ttcttgagcg cctgtgcctc
     1561 gtcgctcacg gcgtcgagaa tactcttctt gcgctcgatc cagtactgtt cctttgcgcc
     1621 gaagaggcgg tcgaacagcc ggtgcggcag catttccggc ggtagcggac gatcacgtcc
     1681 agcccagctc atgttgcgct gaatgctttc cccgaacgat tcctggcata cgccgatctg
     1741 aagggagcgg aagcgactgt tattgccgat ccgctcggcg atcacctggt cgatggacgg
     1801 gccgccagca ccgcggcccg agaacatttc gcccgagacc aggccactca tcgagttgtg
     1861 gtggccattt cccggcccag gcagacgggc cgcgggattg tcgacgccgc tcaagacgtg
     1921 gatgtcctga cgataacgcg ccagcggctg caggcagggg gtcatctcat actgggcacc
     1981 gtcctcgcgc ggaatccagt atttctcgat gattccgttg ccgttgaacc acagcacgaa
     2041 gcgggtctca atcgtctggg cggaaacgcc tgcctcggcc gcgtaggccg tcccgctggc
     2101 gttgaacatc gcctcgaatg ccggcaggcc gacgtgaatc gcggccccag agagccccac
     2161 acccctcaga aacgtgcggc gggacaacgt ttttcgcggt gtcatgagct aacccgagcc
     2221 tttctgaaga aacagttccg acgtcacaac ggcgacgatc aactcccgga aacgaaagcc
     2281 ggaattacaa aacctctcga caagcgcatc gatggtcggc tggtcgctcg cggtctcttg
     2341 ccggccaaac gcataccgga acaactgctt cacgacggcc ttctgacacg tcttactttc
     2401 ggcgagcagg cgtcccagct cgcggggcgt cgagaactcc gaatcttcca tcccctggat
     2461 gaacccggac gtgtcgacgt ccagctcttt tatcatcggc gtgcgcccgc gtgtctgacc
     2521 atcccctggc cccggaaact gaagcaccat cttcttctga aatacgccga tcgcgttgta
     2581 ttgctcgaaa gccagtccga tgggatcgat catgcgatgg caactcgcgc acgactcgct
     2641 gttcaagtga acggccagcc gttgccggtt tgtcagcggc gcgttttcgc tgatagtggc
     2701 gagaacggcg ttcactcccg ccggaggcgg aggaatttcc tgggcgagaa actggttccg
     2761 gacgaacaac cctcgcgcgg tcggcgacgt ttctgcgggc ttactggtca acaacaagaa
     2821 cgcgccatgc cccagcacgc cggcacggcc ggaacccgcc gggtacgcga ccttcgcgaa
     2881 ttcct
//
LOCUS       DATAQL010000895         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4364953, whole genome shotgun sequence.
ACCESSION   DATAQL010000895 DATAQL010000000
VERSION     DATAQL010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4364953"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VHS97_15485"
     CDS             complement(<1..235)
                     /locus_tag="VHS97_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449656.1"
                     /translation="MRPFTYLVFNVPFRSGLVAIVLSMNVTVVALCQEHESNPAAKIQ
                     PETELAIGTEVVFKSSHLPVGDVEQQAQSTIHPP"
     gene            complement(384..>1514)
                     /locus_tag="VHS97_15490"
     CDS             complement(384..>1514)
                     /locus_tag="VHS97_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX3449657.1"
                     /translation="INSTTAGAIGRLCSATRSAHIDENGNADDLLIGLQLTHSGRYSF
                     ALPIMAQHDPLLDPRTVLDKATGATADADSPLISDDELDRLQDRYVEAARLASQAEFD
                     FIDIKQCHRYFLNELLASRTRPGKYGGSFENRTRFIREVIERIRDACPGLLVGTRLNV
                     FDGVPYMKGQDGCGIPCPVTLPASSVWGTRADDPTLPDLAEPLALIGLLRDLGIGLVN
                     VSLGNPYASPHLLRPFEYPPPDGYETPEHPLIGVDRHFRLTAAVQAKYPDLAVVGSGY
                     SWLQAYAFQAGAANVAAGKTSFVGIGRGALTQPDFGRRLAQGEPLDRKRLCRTFSYCT
                     ALMRSKHNQMGQFATGCPPFDKDVYGPIWEQAQQTGSADGKQ"
BASE COUNT          290 a          452 c          478 g          294 t
ORIGIN      
        1 agggaggatg gatcgtgctt tgagcttgct gctccacgtc accgaccggg agatgcgaac
       61 ttttgaaaac cacctcggtc ccgatggcga gttcggtctc gggttgaatc ttggcggcgg
      121 gattactctc atgttcttga cagagcgcga ccacagtcac attcatcgag agcacgatcg
      181 cgacaagccc ggagcggaaa ggcacgttga acacaaggta cgtgaacggc cgcattgttc
      241 cagccctccc gtccagacac cgcgcgcggc tgaactccgc ggagtccaag ggcacgatgg
      301 gcgagcacgt ctcgtcgctt ggtacggcat cgacgagtcg cgggtgacca tgttgcttct
      361 acgttgccat tggcgaacgt ggttcactgt ttcccgtctg cgctcccagt ttgctgtgcc
      421 tgctcccaga tcggcccata gacgtccttg tcaaaggggg gacagccggt ggcaaattgg
      481 cccatctgat tgtgctttga tcgcataagg gccgtgcagt agctgaaagt gcgacagagc
      541 cgtttgcggt ccagcggctc gccctgcgcg agcctgcggc cgaaatcggg ctgagtcaga
      601 gcgccccggc cgatcccgac aaacgacgtc ttccccgctg ccacgttcgc ggcaccagcc
      661 tggaacgcgt atgcctgaag ccagctataa cccgacccga cgaccgccag atcgggatat
      721 ttggcctgga cggccgcggt caggcgaaaa tgtcgatcga ctccgatcaa tgggtgctcg
      781 ggtgtttcgt agccatcggg aggcggatac tcgaacggtc gcaaaaggtg aggactcgca
      841 taaggattgc ccagcgaaac gttcaccagg ccgatgccca agtcccgtag caagccgatc
      901 agggcgaggg gctcggccag atcgggcaag gttgggtcgt cggcgcgggt gccccaaact
      961 gagctcgctg gcagagtaac ggggcagggg attccgcacc cgtcttgccc cttcatgtag
     1021 ggaactccgt cgaagacatt cagccgggtt cccaccagca atcccgggca agcgtcgcga
     1081 atccgctcaa tcacctcgcg aatgaaccgc gttcgattct cgaacgagcc gccgtatttt
     1141 ccgggacgag tccgcgatgc gaggagctca ttgaggaagt agcggtgaca ctgtttgatg
     1201 tcgatgaagt caaattccgc ctggctcgcc aatctggccg cctctacgta ccggtcctgg
     1261 agccgatcga gctcatcgtc cgatatcaga ggcgaatcgg cgtcggcagt agcgcctgtg
     1321 gccttgtcga gaaccgtccg tggatcgagc agcgggtcgt gctgtgccat gatgggcagc
     1381 gcgaacgagt accggccgga gtgagtgagt tgcagcccga tcagcaggtc gtccgcgttt
     1441 ccgttttcgt cgatgtgggc cgaccgcgtc gcgctacaca gtcgtccgat cgctccggcc
     1501 gtcgtcgaat tgat
//
LOCUS       DATAQL010000896         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3910345, whole genome shotgun sequence.
ACCESSION   DATAQL010000896 DATAQL010000000
VERSION     DATAQL010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3910345"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VHS97_15495"
     CDS             <1..497
                     /locus_tag="VHS97_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX3449658.1"
                     /translation="MLEGILSRLSQLGLMLRSPGRGMESARQVPNPGGTGWRLHAMLI
                     DTILDFAGVDYDAIDHKLFVRPILPGQWPQTGIKQLFPCGDVSYRLERPIGGKVYHLN
                     LKTQLKHPVDLEVELTCPDLKELGHWQAFPPTPEPALDSRTGQLRFRITLPSGASEAN
                     WTWG"
     gene            complement(522..1340)
                     /locus_tag="VHS97_15500"
     CDS             complement(522..1340)
                     /locus_tag="VHS97_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449659.1"
                     /translation="MSESQWEGSGGSFTLSWGGRPWTLNVDEPEPGLRSKVDAGFASL
                     LCLHGLAAAGRFEAQVFTPATLVNVERYRSRVQATFAPAGWGGLIVRAAWSPSCGGTG
                     VDLEVQTSATSVGLLRHVEVMVQSQWRRWGASDPGAVLGRWVRPRDPRSAALSYDGRE
                     GADDLHSLTTLPISEGPPIRVFTPPGVAAGIHYIEMVQPNDAARFIRMEPSEPELPLL
                     STLVLRYGLFGHDFEKGVVFRARLRACWVTSDENATEARWLYQKFVAEPPPLGP"
     gene            complement(1579..1866)
                     /locus_tag="VHS97_15505"
     CDS             complement(1579..1866)
                     /locus_tag="VHS97_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449660.1"
                     /translation="MKQGRRFRYIPGCSAPGCVHPAIYKIAAAWSDGTSRELKNYGLV
                     CEAHRDSLLTLARSHHQGLKLSNGESVGPVELYVLRMGCRDVELTRLVDEG"
     gene            complement(1889..2923)
                     /locus_tag="VHS97_15510"
     CDS             complement(1889..2923)
                     /locus_tag="VHS97_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX3449661.1"
                     /translation="MRVAERLQEREKAWHELDLLIMRFGESTRRRPTTGEVLRLGELY
                     RAACADLMLADDHDLPRETVAYLHALVGRAHNLIYRASGFDFRDWGRALFRTAPRRLR
                     SDPALRLSALVFWGAFLMTALLAAGRKDFTARVVGESFVEQFDHMYAEPVNGERQDGA
                     RRSDTMMAGFYIQNNTSIGLRCFAWGIVFGLGSLYQLLSNAIVLGAVFGYMATNPHAA
                     NFFTFVTAHSAFELTAIVVSAAAGLRLGWGLIDTQGQSRVASLRREAVNSLPAVGAAV
                     VLFVLAALVEGYVSASSLPYWSKASVALLSAAAILAYLALGGRADGAGKERDRSDQVT
                     SDKASATAGD"
     gene            complement(2928..3692)
                     /locus_tag="VHS97_15515"
     CDS             complement(2928..3692)
                     /locus_tag="VHS97_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX3449662.1"
                     /translation="MSNHRAQSRREPLDCVVRLVTPERIVIAHPLAGPARRFVAYLID
                     LVLLGAMVFVAFVISLVLSFGATAGLGPAMLALFLLTWGYGAFCEGVFNGQTVGKRAM
                     RIRVVSERGVPISGAQAILRNLVGTVDGLIPFFFQIALVSMTLTRKFQRLGDLAAGTM
                     VVIEERRSRRGITKISGPDVEALLPWLPGRIAAGSDLARVLSDYVEARRRFGPLRRAE
                     MAEPLARPLRKRFGLREQYTADVVLCAVYHRVFMGD"
BASE COUNT          831 a         1265 c         1087 g          637 t
ORIGIN      
        1 tcatgctcga gggcatattg agccgactct cgcagcttgg actcatgctg cggtcacccg
       61 gccgtggcat ggaatccgcc cgccaggttc ctaatcccgg tggaacaggc tggagactac
      121 atgccatgct gatcgacacc atcctcgact ttgctggcgt ggactacgac gcgatcgacc
      181 ataagctctt cgtccggccg atactgcccg gacagtggcc ccaaacgggg atcaagcaat
      241 tgttcccgtg cggcgatgtg tcgtacaggc tcgagcgccc gatcgggggc aaggtgtatc
      301 acttgaactt gaagacgcaa ctaaaacatc cggttgacct cgaagtcgag ctcacctgtc
      361 ccgacctgaa ggagctgggg cactggcaag cttttccgcc gactcccgaa ccggctctcg
      421 attcgcgcac aggtcagttg cgttttcgta tcacgctgcc ctcgggcgcc agcgaagcaa
      481 actggacctg gggttgacca ccaccacgca gcaggctcgc ctcagggtcc cagcggaggg
      541 ggttccgcca cgaatttctg gtacagccac ctcgcttcag tcgcattctc atcggaagta
      601 acccagcaag cgcgcaagcg ggcgcgaaag acgacccctt tctcgaagtc gtgcccgaag
      661 aggccgtacc gcaggaccag tgtggagaga aggggcagct ccggctcgct cggctccatt
      721 cgaatgaacc gcgctgcgtc attcggttga accatctcga tgtaatggat gccggccgca
      781 acccctggcg gcgtgaagac gcggattggg ggcccctcgc tgatcggaag cgttgtcagg
      841 ctatgcaaat cgtcggcgcc ttcgcggcca tcgtagctca aggccgcgga acgaggatcg
      901 cgtggtctga cccagcggcc cagaacagcc ccaggatccg atgcgcccca gcgccgccac
      961 tggctttgta ccatcacttc gacgtggcgg agcaggccca ccgacgtcgc ggaagtctgg
     1021 acttccagat cgacccccgt ccccccgcac gacggactcc aggcggcgcg cacgatcaat
     1081 cccccccaac cggccggagc gaacgtcgcc tggacgcggg aacgataacg ctccacattt
     1141 accaatgtgg ctggagtgaa cacctgagct tcgaaccgcc cagccgcggc cagaccgtgc
     1201 aagcacagga gactggcaaa cccagcgtcg accttcgacc gcagacccgg ctcgggttca
     1261 tcgacgttga gcgtccacgg acgaccaccc cagctcaggg tgaagctccc gccagatcct
     1321 tcccattggc tttcagacac gtctgggctc aaaacaaaac cccgtcgacc cgagtttcca
     1381 cggatcggcg gggccggaac agagaagagg ccggttatga acgaggcaga cacctcggct
     1441 ggaaggatag atacaactgt gaaatcgtcg caaccagcaa cgattaagtt aaacctccca
     1501 cgcgggatgt caagagtgta gcggtctttt ttctactgat ttgttacgga ttggcaacct
     1561 catcggatcc gaaccgcttt atccctcgtc gaccaaacgc gtcagctcga catcgcggca
     1621 tcccatgcgc aagacataaa gctcaacagg cccaaccgac tctccgtttg aaagcttcag
     1681 gccttgatga tgactccgcg ccaatgtgag cagcgagtca cgatgcgctt cgcacaccag
     1741 gccataattc ttgagctcac ggctcgtgcc atcgctccag gccgcggcga tcttgtaaat
     1801 tgccggatga acgcatcccg gcgccgaaca gccgggaata tagcgaaatc gtcggccttg
     1861 cttcatgtcc ttggtccttc cgccgggact agtcgccagc ggtcgcactc gctttgtcgg
     1921 aggtcacctg atcggaacga tcgcgctctt tgccggcacc gtcggctcgt cccccgagcg
     1981 ccaggtaagc aaggatcgcc gcggcgctca aaagggccac cgaagccttt gaccagtatg
     2041 gcaacgacga cgccgataca tagccttcga ccagcgccgc cagaacgaag aggactaccg
     2101 cggctcccac cgccggcaag ctattgaccg cctcgcggcg caaggacgcg acgcgcgatt
     2161 gcccttgggt gtcgattagc ccccagccga gccgcaaacc agcggccgcg gagaccacga
     2221 tggccgtcaa ttcgaaggcc gagtgcgcgg tcacgaacgt gaagaagttg gctgcatggg
     2281 gattggtggc catgtagcca aacaccgctc caagaacgat cgcattggaa aggagttgat
     2341 aaagactccc cagcccaaac acgatgcccc aggcaaaaca gcgcaggccg atcgaggtat
     2401 tattctgaat gtaaaagccg gccatcatcg tgtcgctacg gcgtgctccg tcttgacgtt
     2461 ctccgttgac cggctcggca tacatgtgat cgaactgttc cacaaacgac tcgcccacaa
     2521 cgcgggcagt gaaatccttg cggcccgccg ccagcagagc cgtcatcaaa aacgcccccc
     2581 agaataccaa ggccgacagc cgcaaggcgg ggtccgatcg cagccggcgc ggagcggtcc
     2641 gaaacaaggc acgcccccag tctcgaaagt cgaatccgga agcacgatag atcaagttgt
     2701 gagcgcggcc gacaagcgcg tgcagatatg ccacagtctc ccgcggcaag tcgtggtcgt
     2761 cggcgagcat caagtccgcg caggccgccc gatatagctc gccgagcctc aagacctcgc
     2821 cagtcgtcgg ccgccgcctt gtcgattcgc cgaaccgcat gatcaaaagg tccagctcat
     2881 gccacgcttt ctcgcgctct tgcagccgct cggcaactcg cattgattca gtcgcccata
     2941 aaaacccggt ggtagaccgc gcaaaggacc acgtcggcgg tatattgctc ccttagcccg
     3001 aaccgttttc ggagcggtct cgccagcggc tcggccatct cggcacggcg gagtggaccg
     3061 aaccgccttc tcgcctcgac gtagtcggaa aggacgcggg ccaggtccga gccggcagcg
     3121 atccggcccg gtagccatgg caacaacgcc tcgacgtcgg ggccgctgat tttggtaatc
     3181 ccacgccgcg agcgccgctc ttcgataacg accatcgtgc ccgccgccag atcgcccaga
     3241 cgctgaaact tgcgagtcaa ggtcatgctc accaaggcga tttgaaagaa aaaagggatc
     3301 aacccgtcta ctgttcccac cagattgcgc aagatcgcct gggcaccact gatcggcacc
     3361 cctcgttccg aaacgacccg aattctcatt gcccgtttac ccacagtctg accgttaaag
     3421 acgccctcgc agaaggctcc gtaaccccag gtcaacagaa acagcgccag catcgccggc
     3481 ccgagcccgg cggttgctcc gaatgacagg accagtgaga tcacaaacgc cacgaagacc
     3541 atggcgccca gaagcacgag atcgatcagg taggccacga agcgccgcgc cgggcccgcg
     3601 agcggatgcg cgatcacgat tcgctcggga gtcaccagac ggacgacaca atccagcggc
     3661 tcgcgccggc tctgggcgcg atggttcgac atcctcgtct cctagtcagc tacagagcct
     3721 cgactcggtc cggagcaaca gactcgtgag ctgtacggcc cgcccccgcg gaacacacgt
     3781 ccatgagtat acacacgaga gaattgggct cgcaagatgc
//
LOCUS       DATAQL010000897         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5435944, whole genome shotgun sequence.
ACCESSION   DATAQL010000897 DATAQL010000000
VERSION     DATAQL010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5435944"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VHS97_15520"
     CDS             <1..306
                     /locus_tag="VHS97_15520"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3449663.1"
                     /translation="ITGQWATDDSGGGITLIDGNYNIVGTTSPSGNFTSFTPFTLTGA
                     VTAGLNYLDFVNVNSGGPGGLRVEFQSATVTPEPSSILLGGLGAIGLLIASRRRRKA"
     gene            548..1774
                     /locus_tag="VHS97_15525"
     CDS             548..1774
                     /locus_tag="VHS97_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX3449664.1"
                     /translation="MDSVAGSANGGNFEGVIRIEESKIRSHVDEVVRETVEQTLNGLL
                     EAEADELCGAKRYSRSPERLDTRAGHYDRKLQTKAGEVTLSMPRLRSLPFETQIVERY
                     RRRESSVEEALVEMYLAGVSVRRVEDITEALWGTRVSPSAVSELNQKIYGQIEAWRSR
                     PIEGRHAYVYLDGIWLNRSWGGEVKKVAVLVAIGVREDGYREILGVAEGLKEDTESWR
                     GFLRHLKERGLSGMRLITSDKCLGLVEAVGEFYPEASWQRCMVHWYRNVMSVVPKGKV
                     KEVVAMLKAIHAQEDRQAAHDKAQAVAGKLDVMRLGQAATVVRTGVDETLSYMAFPRE
                     HWTRIRTNNVLERIMREIRRRTRVVGNFPDGKSALMLVAARLRHIAGTKWGRRVYLDM
                     ARLSEVEQAAVGLPVA"
     gene            2082..>2288
                     /locus_tag="VHS97_15530"
     CDS             2082..>2288
                     /locus_tag="VHS97_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449665.1"
                     /translation="MPQAGHVWVSEPDFHRNFPFHEKPQDSLRLTGRLDAALMKEFSV
                     DAGRFYLMGYSNGDCGAWHILVCEL"
BASE COUNT          465 a          622 c          764 g          437 t
ORIGIN      
        1 atcaccggtc aatgggcgac ggacgatagc ggcggcggaa taacgctcat cgacggcaac
       61 tataacatcg ttggaactac gtcgccgtct ggcaatttta cttccttcac accgttcacg
      121 ctgacgggag cggtaactgc cggactcaat taccttgact tcgtcaatgt taattctggt
      181 ggaccgggcg gactgcgggt tgagtttcaa tcagcgaccg tcactcctga gccctcttcg
      241 atattgctcg gtggccttgg ggcgattgga cttctgatcg ctagccggcg ccgccgcaag
      301 gcatagtttc acagaacacg atgccaatca cgaaaaccgc ccggcgattg accatttggg
      361 cggttttttt tgcgcaccca ctgtgccggc tttccctagc gtgcgcgcga cgcgttacga
      421 tgcccatacg tttgcgactt cggacggtga taacgtcctg aatgttgcgc acaaattgaa
      481 ggggcagtcc tggaactggt agaagtttct tgtcagacga actctatcag cacgaaagga
      541 ctgcccgatg gacagtgtag caggatcggc aaatggcggg aactttgagg gcgtgattcg
      601 gattgaagaa agcaagattc gctcgcacgt ggacgaggtg gtgcgcgaga cggtcgagca
      661 gacgctcaat ggcctgctgg aggcggaggc cgatgagttg tgcggagcca agcgttactc
      721 acgatcgccc gagcggctcg acacgcgggc cggccactac gaccgcaagt tgcagaccaa
      781 ggccggcgag gtgaccctct cgatgccacg gctgcggagt ctgccgttcg agacccagat
      841 cgtcgagcgt taccggcgtc gcgagagcag cgtcgaggag gccttggtcg agatgtatct
      901 ggccggcgtc agcgtgcggc gcgtcgagga catcaccgag gcactgtggg gtactcgggt
      961 cagcccgagc gccgtcagcg agctgaacca gaagatttac ggccagatcg aagcctggcg
     1021 gagccgaccg atcgaagggc ggcatgccta cgtgtacctg gacggcattt ggctgaatcg
     1081 gagctggggc ggcgaggtga agaaggtggc cgtgttggtg gcgatcggcg tgcgtgagga
     1141 cggttaccgc gagatcttgg gagtagccga ggggctgaag gaagacaccg agagctggcg
     1201 cggctttcta cggcacttga aagagcgtgg cctttcgggc atgcggctga tcaccagcga
     1261 caagtgcctg ggcctggtcg aagcggtggg cgagttttac cccgaggcga gctggcagcg
     1321 gtgcatggtg cattggtatc gcaacgtgat gagcgtggtg cccaagggca aggtgaaaga
     1381 agtggtggcc atgctgaaag cgatccatgc ccaggaagac cgccaggcgg cccacgacaa
     1441 ggcccaggcg gtggccggga agctcgacgt gatgcggctg gggcaagcgg ccacggtcgt
     1501 gcggacgggc gtggacgaga cgctgtcgta catggcgttc ccgcgcgagc actggactcg
     1561 catccgcacg aacaacgtgc tggagcggat catgcgtgag atccggcgtc ggacgcgtgt
     1621 ggtgggcaac ttccccgacg gcaagagtgc cttgatgttg gtggcggccc gactgcgtca
     1681 tatcgcgggc acgaaatggg gccggcgtgt ctacctcgac atggcccgcc tgagtgaagt
     1741 ggagcaagcc gcggtcggat taccggtcgc gtaaactggc gaatttttat tccccctcgg
     1801 gggaataatg acagacgaaa acagacgacg aggagcccag ttccaggcac tagccaaaat
     1861 caaatgtgcg caccttgacg gacgctaccc ggacggtaag acgcagcctt acggattgct
     1921 caagcactac ggtaacgata agagccggcg ctatccgcta gtgttgctgc tgcacggttt
     1981 tggcgaccgc ggcaccaaca atgagaggcc agtaaagtcg gacaacaacg gcgcgggctt
     2041 gttcaccaag accgccattc gcgtttcctt gtttcgtcct cgtgccgcag gctggacacg
     2101 tttgggtttc ggagccggat tttcaccgta atttcccgtt tcacgaaaag ccgcaagatt
     2161 cgctccgcct gaccggccgg ctcgacgcgg cactcatgaa ggaattctcg gtcgatgccg
     2221 gccggttcta tctcatggga tactcgaacg gtgattgcgg cgcttggcat atcctggtgt
     2281 gcgagctg
//
LOCUS       DATAQL010000898         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1924348, whole genome shotgun sequence.
ACCESSION   DATAQL010000898 DATAQL010000000
VERSION     DATAQL010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924348"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VHS97_15535"
     CDS             complement(<1..358)
                     /locus_tag="VHS97_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449666.1"
                     /translation="MPNLRSRSQTAIEVRWFNPGLRDSGVPMHCSRFFLENFVGLFAA
                     LILPFLTLPVDARGDEPPLERVRTIALKGPTGGLDHLALDAKRARLLIANAVNDSLDV
                     VDLKGGKVMTIIAGSVA"
     gene            complement(321..1253)
                     /locus_tag="VHS97_15540"
     CDS             complement(321..1253)
                     /locus_tag="VHS97_15540"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEX3449667.1"
                     /translation="MSLLMKMVAPTAITSVLVAGLAFSAAIAPEERLDAAKIAAASGA
                     KTAVTPDGVVRIGWPRDDVKVHVDDVPLKPFAGLGTWAAFSAASHGAMVMGDTVVFQD
                     EVTPAIDAAFAHGLEVTGLHNHFFYDEPKVYFMHIGGQGEPEKLATAVKAVWDAVKKV
                     RAQNPEPATTFREIGPEADAIDPKTVEEILGHKAQSQDGVVKVTIGRDGTMHGVKVGG
                     SMGLTTWAAFSGGDAHAVIDGDFIMTAAEVQPVLRALRKADIHIVALHNHMVGEQPAF
                     YFTHFWGKGPAKDLARGLRSVLDAQLAVAKSDGH"
     gene            complement(1401..1700)
                     /locus_tag="VHS97_15545"
     CDS             complement(1401..1700)
                     /locus_tag="VHS97_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449668.1"
                     /translation="MSAKVEVHVGRCRDVLAVPSEAVSVDQNHSVCYVIGPSGLERRE
                     ITPGGSTLDLIEVTDGLNEGESVVLNPTRVFDRSASRADSAGPNLREGPGLTALR"
BASE COUNT          336 a          628 c          594 g          376 t
ORIGIN      
        1 tcgcaacact ccctgcaatg atcgtcatca ctttcccgcc tttcaaatca acgacgtcga
       61 ggctgtcgtt gaccgcgttc gcgatcaata atcgggcccg tttggcgtcg agcgccaggt
      121 ggtcgagccc gccggtgggt ccctttagag cgatggtcct tacccgctcg aggggcggtt
      181 cgtcgccgcg ggcatcgacc ggcaaggtca aaaacggcaa gatcagggca gcgaacagac
      241 cgacgaagtt ctcaaggaaa aagcgactgc aatgcatcgg cacccccgaa tctctcaaac
      301 ccggattgaa ccatctgact tcaatggccg tctgacttcg cgaccgcaag ttgggcatcg
      361 agaacgctcc tcaatccccg ggccaagtcc ttggccggtc ctttgcccca aaagtgggtg
      421 aagtagaagg ccggctgttc accgaccatg tggttgtgca gtgcgacgat gtggatgtcc
      481 gccttgcgca gggcgcggag cacgggttgg acctccgcgg cggtcatgat gaaatcgccg
      541 tcgatgactg catgggcgtc gcccccgctg aatgcggccc aggtcgtaag gcccatcgag
      601 ccgccgacct tgacgccgtg catcgtcccg tcgcgtccga tcgtgacctt gaccaccccg
      661 tcctggctct gagccttgtg gcccaggatc tcctcgaccg tcttgggatc gatcgcatca
      721 gcctccggcc cgatctcccg aaacgtggtg gcaggctcgg ggttctgggc tcggaccttc
      781 ttgacggcgt cccagacggc cttgacggcg gtggcgagct tttccggctc gccctggccg
      841 ccgatgtgca tgaagtagac cttcggctcg tcgtagaaga agtggttgtg cagcccggtg
      901 acttccagcc catgggcgaa agcggcgtcg atcgctggcg tcacctcgtc ctggaatacc
      961 acggtgtcgc ccatgaccat tgccccgtgc gacgcggcgg agaaggccgc ccacgtgccg
     1021 aggccagcga acggcttgag cgggacgtca tcgacgtgta ccttgacgtc atcgcgcggc
     1081 cagccgatgc ggaccacgcc gtcgggcgtc accgccgtct tggcgcctga ggcagcggcg
     1141 atcttggcgg catcgaggcg ttcctccggc gcaatagcgg cggagaaggc tagacccgcg
     1201 acgagaacac tcgttatcgc ggttggggca accattttca tcaagaggct catcgcacgt
     1261 ctcctttatt cgattcagtg ctttcggtgt catatcgaaa tatcattaat tatggcttat
     1321 cttctgtcct gatgcggcgc ggcccagcca ttccaacgcg cgaccgctgt tgtcagtctc
     1381 tcctgcgccg agcaagtgaa tcagcggaga gcggtgagtc cgggcccttc gcgcagattt
     1441 gggcccgctg aatcggcccg cgaggcagag cggtcgaaaa cccgtgtcgg gttcagtacg
     1501 accgactccc cctcgttcag tccatcggtc acctcgatca ggtcgagggt ggacccgccg
     1561 ggcgtgatct cgcgacgctc caagcccgac ggtccgatga cgtagcaaac actgtggttc
     1621 tggtcgacgc tcaccgcctc gctggggacc gccagcacat cgcggcagcg tccgacgtgg
     1681 acttcgacct tcgccgacat gccggggaga agccctgaag gggtcactgt agcggcgata
     1741 ggtatcgagc tccatctctg cctgcttcaa ccggagggtc gagctcagga gcgcctcgcg
     1801 gtcgttggcg acctgcctca gcgaggcata acccttcgcc gccatccgct ctgaccaggc
     1861 gagccggtcg gacgcggcct tcatctctgc ctctgccagc gcgatccgtc cctgcatgcc
     1921 catgatatct tgag
//
LOCUS       DATAQL010000899         4820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1161331, whole genome shotgun sequence.
ACCESSION   DATAQL010000899 DATAQL010000000
VERSION     DATAQL010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161331"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VHS97_15550"
     CDS             <1..696
                     /locus_tag="VHS97_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449669.1"
                     /translation="PLAPRGLRSLLGDEYFQNVRQVNLVYDDSAGKRLDNTSLQACDD
                     LLKQISTLPGLKGLLLKETQATDEGLRYIGKMTDLEELYIWDAKFITDAGVSHLSRLT
                     NLKKVHIGNSNLTDDSLARISSLPRMEEMSLQQNHFSDEGLARLSGKERLKRLCIGLG
                     DVRITDGGLAHLAGFEKLELLDLQKSRVTDQGLEQLKRLPNLKELWLSETSVSDQAVQ
                     RLRETIPSLKVMR"
     gene            complement(977..1792)
                     /locus_tag="VHS97_15555"
     CDS             complement(977..1792)
                     /locus_tag="VHS97_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX3449670.1"
                     /translation="MVKLRVRGRRKTPKPRDQRPASVFEQLLGWCVHGYTALGLVAAG
                     LIAVLLVRGGPEAFRWSFLLMAAATIVDSTDGTLARLVRIKEVVPTFDGRRLDDIVDF
                     LNYTFLPLLLIWRAEILPPGQEPWLFLPLLASVYGFCQVQAKTDDGYFLGFPSLWNLV
                     ALYLYVLPLPGYVSLGIVIVLALLTFVPIRHLYPSQPGLLNRVSMIVGVLWTPLVGWL
                     IWKLPGAGESRVSDSTLQIAFVSLTYPIYYIAVSWAISVIHWRKRWMNRRTRV"
     gene            complement(1858..3489)
                     /locus_tag="VHS97_15560"
     CDS             complement(1858..3489)
                     /locus_tag="VHS97_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3449671.1"
                     /translation="MTSDTRDLAAFGYKQVLDRTLGSFSSFAAGFSYISILTGVFQMF
                     YVGYGAGGPAFYWTWPMVFLGQITVALCFAELAAHYPLSGGIYQWSRWIGARGVGWMA
                     GWVYLCGSIISLAAVALALQGTLPQIAPAFQLIGDSADKSDSAKNAVLLGCVLIGLTT
                     AINSVGVRLMARINNVGVIAELLGVTVLIVLLAVHIRRGPAVLWDTQGRGAGQPGGYL
                     GPFLAAALMASYVLYGFDTAGAFAEETDLPRRRAPWAILQALAAAGLAGAIVMLFGIL
                     AVSDPALVELGQISGGLPFLVKDVLGPGLGVFLLVEVVFAVFVCALAVHAGTVRLIFA
                     MARDNKLPFAHALAHVQSWSKAPIVPSIVVGVLAAAILVVNFNLPNVFETLCSVAIVW
                     ANLAYLLVTTPLLWARLRRGRDGAFTAAGGELQAPGEPTEDLDVQAPRYFSLGRWGLP
                     VNAIAVAWGLFLVINISWPRPLIYGSGGWGRFAAVFATLGVIVAGTLYFLLFQRRRTG
                     ILEEHAAEPFVDSESILADDSALGARWIGHLAPGE"
     gene            complement(3489..4298)
                     /locus_tag="VHS97_15565"
     CDS             complement(3489..4298)
                     /locus_tag="VHS97_15565"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3449672.1"
                     /translation="MTTTQAASAATPKFDNHGQYSRTGILRYEMIFGEGYISTGGAAT
                     TDDLCNRLGSSLRPGVRVLDVGSGIGGAAFHLADVYGAKVTGIDLAEEMVNIALERAS
                     QRGMSESVKTLLGDVLETSFPEPFDVIWSRDAFMHIPDKPRLFSRLYQLMAPGGRLVI
                     TDYARGKAPGSAEFESYIEKTGYNVIEPREYGRLLEGAGFTNVIVDDATPRFIEILKS
                     EADRLVTKRGEFLVSFSEADLNYLVERWAMKVGFCQAGDMKWGIYLASKKS"
     gene            complement(4548..>4820)
                     /locus_tag="VHS97_15570"
     CDS             complement(4548..>4820)
                     /locus_tag="VHS97_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449673.1"
                     /translation="VQELELAGTVAGKTSLSGSVRRSRGSSGRTVLPPITGSSGVRIK
                     LKPAQFGFSTAAAIDEQTQAASAAVVQKSFGPISRVTPARRVRRKV"
BASE COUNT         1114 a         1478 c         1421 g          807 t
ORIGIN      
        1 cccctggcac cgcgtgggct gcggagcctg ctcggagacg aatacttcca gaacgtcagg
       61 caggtgaacc tggtttacga tgattcggct ggcaagcgac ttgacaatac gagcctccag
      121 gcatgcgatg atctcttgaa gcagatttca acgctgccgg gcctgaaggg gctgttgctg
      181 aaagaaaccc aggcaacaga tgaggggctg agatatatcg gcaagatgac cgacctcgaa
      241 gaactctaca tctgggacgc caagtttatt acagacgcgg gagtatccca tctttccaga
      301 ctcacaaacc tgaaaaaggt gcacatcggc aattccaatc tgacggacga tagcctcgcg
      361 cgtatcagca gtctgccgag gatggaagaa atgtcactcc aacaaaacca tttctcagac
      421 gagggcctgg ctcgtctgag cgggaaggag aggttgaaac gcctgtgcat cggcctcgga
      481 gacgtccgga tcaccgatgg cgggctcgct catttggcgg gctttgagaa actcgagctt
      541 ttggatcttc agaagtccag ggttactgac cagggattag aacagctgaa gcgacttccg
      601 aatctcaaag aactctggtt gagcgagacc agcgtgtccg accaagcagt gcagaggtta
      661 cgagagacga tccctagcct gaaagtaatg aggtaactag catccgaccg attcggacac
      721 aggttataca gcggcggtga tgtttttctg cttgccgcgc ctaaggccat ctgtagattt
      781 gaggcaatcg catggttcgc atccggaggc tctaccggtg ggcctgcacg cgacgagtcg
      841 cgtttcggtc caccctacga tggtgccaca tcaatggacg agaatatcct tcgatgaccg
      901 tctggagtag ggtgggccga acgacgtgag gcccaccaga atgttgagcc gaacgacgtg
      961 aggcccacca gaatgttcac acacgtgttc gacggttcat ccatcgcttc cgccagtgaa
     1021 tcacgctgat cgcccacgag accgcgatgt agtaaatcgg atatgtgagt gagacgaacg
     1081 cgatttgcaa ggtcgaatcg ctcacgcgcg actcgccggc gccgggcagc ttccagatca
     1141 gccagcccac gagcggagtc cacagcacgc cgacgatcat gcttacccgg ttcaataatc
     1201 cgggctgcga cggatagaga tgacggatcg gcacgaacgt caagagagcc agcacgatca
     1261 cgatcccgag cgagacgtaa ccgggcagcg gcagcacata aaggtaaagt gctaccagat
     1321 tccagagcga cgggaaccca aggaaatagc cgtcgtctgt cttggcctgg acctggcaaa
     1381 agccgtagac actcgccaag agaggcaaga aaagccacgg ctcctgcccg ggcggcagaa
     1441 tctccgcgcg ccagatcaag agtaaaggca ggaacgtgta attgaggaag tcgacgatgt
     1501 catccaaacg ccggccgtcg aaggtcggta ccacttcctt gatccggacg agccgcgcga
     1561 gagtgccgtc ggtcgagtcg acgatcgtcg ccgcggccat caaaaggaac gaccagcgaa
     1621 acgcttccgg cccgccgcgc accaacaaca cggcgatcaa ccccgccgcc accaacccca
     1681 gcgcggtgta cccgtgcacg caccaaccca aaagctgctc gaataccgaa gccggtcgct
     1741 gatcgcgcgg tttcggcgtc tttcgtcgac cccgcactcg cagcttgacc atggccgccg
     1801 atctcctcgc gattcgctgg agtcccgatt cgttggttcg catagtgggc gaactcccta
     1861 ttcaccgggc gcgaggtgac cgatccagcg cgcgcccaac gcgctgtcgt cggcgaggat
     1921 ggactcagag tccacgaacg gctcggctgc gtgttcctcc agaatgccgg tccgcctgcg
     1981 ctgaaacaag agaaagtaga gtgtaccggc aacgatcacg ccaagcgtgg caaaaaccgc
     2041 tgcaaagcgg ccccagcctc ccgagccgta gataaggggg cggggccaac tgatattgat
     2101 gacgaggaac agaccccagg ccaccgcgat cgcattcacc ggcaaccccc atctgcccag
     2161 cgaaaagtac cgaggcgctt ggacgtcgag atcttcggtc ggttcgccag gagcttggag
     2221 ctcgccccca gcggcggtga aagcgccgtc gcggccacga cgcaaacggg cccacagtaa
     2281 tggcgtcgtc acgagcaggt acgccaggtt agcccagacg atggcgaccg agcagagcgt
     2341 ctcaaaaacg ttgggcaggt tgaaattgac gaccaggatc gcggcggcca gcacgccgac
     2401 gacgatggac gggacaatgg gggccttcga ccacgattga acgtgggcca gcgcatgggc
     2461 aaaggggagc ttgttgtcgc gtgccattgc gaagatcaac cgaaccgtgc cagcgtggac
     2521 tgccagcgcg cacacaaaaa cggcgaaaac cacctcgacg agcagaaata cacccaaccc
     2581 cggccccaag acatccttga ccagaaacgg cagcccgcca ctgatctggc cgagctcgac
     2641 aagcgcgggg tcgctgaccg cgaggatgcc gaaaagcatg acaatcgcgc cggccaaacc
     2701 ggcggcagcc aaggcctgga ggatcgccca gggagcgcgg cggcggggca agtcggtctc
     2761 ttcggcgaag gcgcctgccg tgtcgaaccc gtacaaaacg tatgaggcca tgagcgcagc
     2821 agccaggaat gggcccaggt agccccccgg ctggcccgcg ccccggccct gcgtatccca
     2881 aagcaccgcc ggtccccggc ggatgtggac cgccagcaag acgatcagga ctgtcacccc
     2941 caaaagctcg gcaatcacac cgacattatt gatccgtgcc atcaagcgca cgccaaccga
     3001 gttgatcgcg gtcgtcaggc cgatcaagac acaaccaagc agaacggcgt ttttggcgct
     3061 atcggacttg tccgccgaat caccgatcaa ttgaaacgcg ggcgcaatct gcggcagagt
     3121 tccctgcagc gccagcgcga cagcggcaag gctgataatc gaaccgcaca agtacaccca
     3181 gcccgccatc cagccgactc ctcgcgcacc gatccaccgc gaccactggt agattccccc
     3241 agacagcgga taatgtgccg ccagctcggc aaagcaaagt gcgacggtga tctggcccag
     3301 aaagaccatt ggccaggtcc agtagaatgc cggcccaccc gccccataac ccacgtaaaa
     3361 catctggaat acgccggtca ggatcgagat gtacgagaag ccggcggcga acgacgaaaa
     3421 actgccgagt gtccggtcaa gcacctgctt gtaaccaaac gccgccaggt ctcgtgtgtc
     3481 tgaggtcatc acgacttttt cgaggccagg taaatacccc atttcatgtc gccagcctgg
     3541 caaaacccta ccttcatcgc ccaccgctca accaggtagt tgaggtcggc ttcggaaaac
     3601 gacaccagga attcgccgcg cttggtgacg agtctatcgg cttcgctctt caagatctcg
     3661 atgaaacgcg gggtggcatc gtcgacgatg acattcgtaa accccgcccc ttcgagcagg
     3721 cggccatact cgcgcggttc gatgacgttg taaccggtct tttctatata actctcaaac
     3781 tcggcagacc caggcgcctt gccgcgcgcg tagtcggtga tgaccagccg gccgccgggg
     3841 gccatgagct gataaagccg ggaaaacagc cttggcttgt cagggatgtg catgaacgca
     3901 tcgcggctcc agatgacgtc aaacggttcg gggaacgacg tctccaggac atctccaagc
     3961 agagtcttta ccgattcgct catcccccgc tgcgacgcac gctcaagcgc gatattgacc
     4021 atctcctcgg caagatcgat gccggttacc ttggcgccgt agacatcggc caggtggaag
     4081 gcggcgccgc cgatgccgct ccccacgtca agcacgcgga cgccgggccg cagcgacgaa
     4141 cccaggcgat tgcacaggtc atcggtcgtg gccgctcccc ccgtgctgat atacccttcg
     4201 ccaaagatca tctcgtatct caatattccc gtgcggctgt attgaccgtg gttgtcgaac
     4261 ttcggagtcg cggccgaagc cgcctgcgtc gttgtcatga aatttctcaa ttcaaatcta
     4321 atgccgagcc attcgccgtg cgatgatcga aatggaactc ggttggatgg cagcatgaac
     4381 agttgcgcga ttttgcgata attattatgt gagaggcgga agttgaatgt aaacagcgga
     4441 gtgtaagcgg gcgcctgagc gcgaggtcgc gtaatcgcat ggtcgccgca tgccagcgat
     4501 gaaggtcggg gtagggatcg atccagcggg tcgcagcccg gacgcgctca taccttacgt
     4561 cgcacgcgcc gcgccggcgt tactcgcgag atgggtccaa aggatttttg gaccacggcc
     4621 gccgacgctg cctgggtttg ctcatcaatc gcagcagcgg tcgaaaagcc aaactgcgcc
     4681 ggcttgagct tgatccgaac cccggacgag ccagtgatcg gaggcaatac cgtgcggccg
     4741 gatgagccgc ggctacggcg caccgagccg ctcaggctcg tcttgccagc gaccgtgccg
     4801 gctagctcaa gctcctggac
//
LOCUS       DATAQL010000900         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_92249, whole genome shotgun sequence.
ACCESSION   DATAQL010000900 DATAQL010000000
VERSION     DATAQL010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92249"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VHS97_15575"
     CDS             complement(<1..931)
                     /locus_tag="VHS97_15575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3449674.1"
                     /translation="MQYRSFGRLGWLVGEIGYGMWGLAGWSGSDDDETSAALERAVEL
                     GCNFFDTAWAYGNGHSERLLGALVRNHTGKELYVATKVPPKNFKWPSRRGYTLADCFP
                     ADHIREYAEKSLANLDLPRIDLLQFHVWEDSWARDEEWQRAIEDLKRQGKIRAAGVSV
                     NRWEPANVVETLRTGLVDAVQVIFNIFDQSPQDELFPVCRELNVAVIARVPFDEGTLT
                     GNLTKTSRWPQGDWRNSYFVPENLAQSVDRAEALRPLVPAGMTMPELALRWILADEAV
                     STIIPGMRKVKHVAANLAASDGRPLEPDLLDRLK"
     gene            complement(1011..3746)
                     /locus_tag="VHS97_15580"
     CDS             complement(1011..3746)
                     /locus_tag="VHS97_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX3449675.1"
                     /translation="MCPHPTAEHVRLSDSAARRADWKNWGPYVAERAWGTVREDYSAN
                     GDAWNHFPHDHARSRAYRWNEDGLAGYCNRFQNLCMAVALWNERDPFLKERLFGLANE
                     EGNHGEDVKEYYYYLDGVPSHAFMRMLYKYPQVEYPYRLLVEENRRRGRASPEFELVD
                     AIGGALAEGRYFDVYIEYAKASQEDILCKITAHNRGPDPAPLHVLPQIWFRNTWSWGY
                     RARRPELEAAGPTVVRAFHRHLGERFWYLDIQPHAPALVFTENETNFQRLFGVPNAGP
                     FVKDAFHDAIVGGLLDKVNPKQKGTKAAAHYSGMVAAGGSLTIRARFTNSQEGDPFGD
                     FDAIVERRLAETDDFYRSIQPAGLGEDETRVQRQAFAGLLWTKQFYHYSVELWLDGDP
                     AGPEPPARRKHGRNAQWRHVYNLDVLSMPDKWEYPWFAAWDTAFHCIVLARLDPEWAK
                     RQVLLLVREWYMHPSGQLPAYEWDFGDANPPVHARAARRVYEITRELTGTADTDFLEE
                     VFHKLLLNFTWWVNRKDPEGRNAFQGGFLGLDNIGVFDRSNPNLGEDRRLEQADGTAW
                     MGMFCLDMLAIALELSQSRPAYESIATKFFEHFVAISHAINGFGGEIGMWDPDDGFYY
                     DVVRVKGGEPQHLKVRSFVGLIPLFAVLAVEPGTLERLPHFRRRVEWYIRYRPMLAGN
                     LCLMMNPGHGSRRLMALADRAKLESVLPRVLDPSQFLSEFGVRSLSRALADEPFVYNG
                     NRVTYEPAESASPIYGGNSNWRGPIWFPVNYLLVESLRELHSYYGSTFKIELPRGTGR
                     QATLEEVAAMVAGRLARIFLRDSSGRRPVFGGQELFHSDPHWRDHIPFYEYFHGDNGS
                     GLGASHQSGWTSLVADLIAWRHAWATAQINSAPAKPSVFPAPASAKLGPTPGRSC"
     gene            complement(3758..4927)
                     /locus_tag="VHS97_15585"
     CDS             complement(3758..4927)
                     /locus_tag="VHS97_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX3449676.1"
                     /translation="MSDTIASARIAGPEAIHGCPSHIDHDRKLPEIDLLSPLTIRGVG
                     FRNRIVMSPMCQYSATEGLANDWHLVHLGSRAAGGVALVMVEATAVTRDGRISPGDMG
                     IWEDRHIEPLARIARFVHSQGAVAGIQLAHAGRKASCDVPWKGGASLKTPKEVGWTVV
                     GPSAIPFSEGDPVPVPLDEAGIEGVVAAFEAAARRALAAGFCVIEIHSAHGYLLHEFL
                     SPLSNHRQDQYGGNLENRMRLLLRVVTAIRRLMPDNLPLFVRISATDWADGGWDADQS
                     VILARRLKELGVDLIDVSSGALTPKARIPVAKGFQVPFARRIRDEAGIKTGAVGMITE
                     PAHANEIITEGDADLVFVARELLRQPYWALRAEQELGADPSWPIPYGYAVRRRAK"
BASE COUNT         1022 a         1618 c         1541 g          942 t
ORIGIN      
        1 ccttgagtcg atccagcaaa tccggctcga gcggccggcc gtcgctggcg gccaagttgg
       61 cggcgacgtg cttgaccttg cgcataccgg ggatgattgt cgacaccgcc tcgtccgcga
      121 ggatccagcg caaagcaagc tcgggcattg tcattccggc cggcacgagc ggccgaagag
      181 cctccgcgcg atcgacgctc tgggcaaggt tttccggtac gaaatagctg ttgcgccagt
      241 cgccttgtgg ccaacggctg gtcttggtca agttgcccgt caaggtaccc tcgtcgaacg
      301 gaacgcgcgc gatcacggca acgttgagct ctcggcagac gggaaacaac tcatcctggg
      361 gagactggtc gaagatattg aagatgacct gaaccgcgtc gaccaagccg gtccgcaacg
      421 tctcgacgac attggcgggc tcccaacgat tgacgctgac cccggcggcg cggatcttcc
      481 cctggcgctt tagatcctca atcgcgcgct gccattcttc gtcgcgggcc caggaatctt
      541 cccagacgtg gaactggagc aagtcgatcc ggggcagatc gagattcgcc agactctttt
      601 ccgcgtattc acgaatgtgg tcagccggaa aacagtcagc gagcgtgtag ccgcggcgcg
      661 acggccactt gaaattcttg ggtggcactt tggtggcgac ataaagctct tttccggtgt
      721 gattgcggac caaggcgccg agcaggcgct cactgtggcc gttcccataa gcccaggccg
      781 tgtcgaagaa gttacagccc agctcgacgg ctcgttcaag agccgcggaa gtttcgtcgt
      841 catcggaccc agaccagccc gctaaacccc acatgccgta gccgatctcg ccaacgagcc
      901 agcccaatcg accaaagctc cgatattgca tgacaaaaat ccctcaagat gagatcgacg
      961 gcggcgtgag catccgtcaa cagcctcgat cgatacgcgt ccgatcgtcg tcaacacgac
     1021 cggcccggcg ttggccccag tttagcagat gcgggtgctg gaaaaactga cggctttgcc
     1081 ggggcgctat tgatttgagc ggtggcccac gcgtgcctcc acgcgatcag gtcggcgacc
     1141 agagaggtcc agccgctctg gtggctggcc ccaaggcctg agccgttgtc gccgtgaaag
     1201 tattcataaa acggaatgtg gtcgcgccaa tgtggatcgg aatggaaaag ttcctgacca
     1261 ccgaaaacgg gacgccgacc ggacgagtca cggagaaaga ttcgcgccag tcgacctgcg
     1321 accatggcgg caacttcttc cagcgtcgcc tggcggcctg tgccgcgggg cagttcgatc
     1381 ttgaaggtcg agccatagta actgtgcagc tcccgcagcg attcgaccaa caaatagttg
     1441 acagggaacc agatcggtcc gcgccagttg gagtttcccc cgtaaatcgg cgaggcggac
     1501 tcggccggct cgtacgtgac ccgattgcca ttgtagacaa agggctcgtc ggccaaggcc
     1561 cgcgagagcg accgtacgcc gaactcggaa aggaattgcg acgggtcgag aacccgaggc
     1621 aggacacttt cgagctttgc ccggtccgcc agcgccatca gtcgacggct accatgtccc
     1681 ggattcatca tgaggcagag gttgcctgcc agcatcggcc gatagcgaat gtaccactcg
     1741 acccgccggc gaaagtgagg cagcctctcg agtgtacccg gttcgaccgc caggaccgcg
     1801 aagaggggga tcaaacccac gaacgagcgc accttgaggt gttgcggttc cccccctttg
     1861 acccgaacca catcgtagta gaatccatcg tcagggtccc acatgccaat ctcgccgccg
     1921 aatccgttga tcgcgtgcga gatcgccacg aagtgctcga aaaacttggt ggcgatcgat
     1981 tcgtaagccg gccgggattg cgataattca agcgcgatcg ccagcatgtc gaggcagaac
     2041 attcccatcc aagcggttcc gtcagcctgc tcgagccgtc gatcttcccc caagttggga
     2101 ttgctgcggt cgaaaacgcc gatgttgtcc aggccaagga agcccccttg aaacgcgttg
     2161 cgtccctcgg gatccttgcg gttaacccac caggtgaagt tgaggagcag cttgtggaac
     2221 acctcctcga ggaagtcggt atccgccgtg ccggtcagtt cgcgcgtgat ctcataaacc
     2281 cgccgggcgg cgcgagcatg caccggcgga ttggcgtcgc caaagtccca ctcgtacgcc
     2341 gggagctggc cgctcgggtg catataccac tcgcgcacga gcagcagcac ctgccgcttg
     2401 gcccattccg ggtcgagccg ggcaagcacg atgcagtgaa acgcggtatc ccaagcggcg
     2461 aaccatgggt attcccactt gtcgggcata ctcagaacgt cgaggttata cacatgccgc
     2521 cactgcgcgt ttcgaccgtg cttccgccgc gccggcggct ctggaccagc cgggtcgccg
     2581 tcgagccaga gctccacgct gtagtggtaa aactgcttgg tccatagcaa acccgcaaac
     2641 gcctggcgct gaacgcgggt ctcgtcttcc ccgagcccgg ccggctggat ggaccggtaa
     2701 aagtcgtcag tctcggcgag ccgacgctcg acgatcgcgt caaagtcgcc aaaagggtcg
     2761 ccttcctggg aattcgtgaa tcgcgccctg atggtcagag atccgccggc cgcgaccatt
     2821 ccgctatagt gagccgccgc tttcgtgcct ttctgcttgg gattcacctt gtcgagcagt
     2881 ccaccaacga tcgcgtcgtg aaacgcgtcc ttcacgaaag gtccggcgtt gggcactccg
     2941 aaaagccgct ggaaattcgt ctcgttttcg gtaaaaacga gcgccggagc gtgcggctgg
     3001 atgtcgagat accaaaagcg ctcgcctaga tgccggtgga acgctcggac gaccgtcggc
     3061 ccggcagcct cgagttccgg gcgccgggcg cgataacccc aagaccaggt gttgcgaaac
     3121 cagatctggg gcaagacatg cagtggggcc gggtcgggac ctcggttatg tgccgtgatc
     3181 ttgcacagga tgtcttcctg gcttgccttg gcatactcga tatagacgtc gaaataacgc
     3241 ccctcggcga gagcaccgcc gatcgcatcg accagctcga actcgggcga agctcggccc
     3301 ctccgccggt tttcctcgac aagcagacga taggggtatt cgacttgcgg atatttgtat
     3361 aacatccgca taaaagcatg gctgggcact ccgtccaggt aatagtagta ttccttgacg
     3421 tcctcgccgt ggttgccttc ttcgttggcc aaaccaaaga gacgctcctt gagaaacggg
     3481 tcgcgctcgt tccagagcgc gactgccatg cagagattct gaaagcggtt gcaatagccg
     3541 gcgagaccgt cttcgttcca gcgatacgcg cggctgcgcg cgtggtcgtg cgggaaatgg
     3601 ttccaggcat cgccgttagc gctatagtct tcgcgtactg tgccccaggc ccgctcagcc
     3661 acataaggcc cccagttttt ccagtcggcg cggcgagcgg cgctatcgct caggcggacg
     3721 tgctcggcgg tcgggtgtgg acacacgaat ccgatcctca cttcgcccgc cggcgcaccg
     3781 catagccata ggggatcggc cagctcggat cggcgccaag ctcttgctcg gcccgcagcg
     3841 cccagtaagg ctggcgtaac agctcccgag ccacgaaaac gaggtcggca tcgccttcgg
     3901 tgatgatctc attggcatgc gcgggttcgg tgatcatgcc gaccgcgccg gttttgatcc
     3961 cggcctcatc acggatcctg cgcgcgaacg ggacctgaaa acccttggcg accggaattc
     4021 gcgccttcgg tgtcagggca ccggacgaga catcgatcaa gtccacacct agctccttga
     4081 gccggcgagc cagaatcacc gattgatcgg cgtcccaacc tccgtctgcc cagtccgtcg
     4141 ccgagatccg cacgaagagc ggcaggttgt ccggcatcag acggcggatt gcggtcacga
     4201 cccgcaacaa tagccgcatg cggttttcga ggttgcctcc atattggtcc tggcggtgat
     4261 tggagagtgg cgaaagaaac tcgtgcaaga gatatccatg ggcggaatgg atctcgatca
     4321 cacagaaacc agccgcgagc gcgcggcgcg cggcggcctc gaatgcggcg actacacctt
     4381 cgatcccagc ctcatccagc ggcacgggga cagggtcgcc ctcggaaaac ggaatcgcac
     4441 tgggccccac gaccgtccag cctacttcct tgggcgtttt caagctcgca ccacccttcc
     4501 agggcacgtc gcagcttgcc ttccggcctg cgtgagcgag ttggatcccg gccaccgctc
     4561 cctgactgtg aacgaaccga gcgatccgcg ccaaaggctc gatgtgccgg tcttcccaga
     4621 tccccatgtc accgggcgag atccggccgt cgcgcgtgac cgccgtcgct tccaccatca
     4681 cgagcgccac gccgccggcg gcccgacttc ctaggtggac caagtgccaa tcattagcca
     4741 gaccttccgt cgccgagtac tggcacatgg gcgacatcac gatccggtta cgaaatccca
     4801 cgccccgtat cgtcaatggg ctcaagagat cgatctcagg aagcttgcgg tcgtggtcga
     4861 tgtgcgaggg acagccgtga attgcctcgg gtcccgcaat gcgggcactc gcgattgtgt
     4921 cactcatcgt gatcgctccg tcaccactgt ctgggaaaat cgtagatcca ggaactgtcg
     4981 ccagatcgac cgcaccgcaa aagtgtacgc ctgcaactta gatgtcgaat cagagtcctt
     5041 acaccgcggt ggcagaacat gacccaccgc ccggaggagt ggttcggctt gccgatgggc
     5101 ggtccgtcgc gttacgctag cgt
//
LOCUS       DATAQL010000901         6309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_397146, whole genome shotgun sequence.
ACCESSION   DATAQL010000901 DATAQL010000000
VERSION     DATAQL010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397146"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VHS97_15590"
     CDS             <1..1526
                     /locus_tag="VHS97_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3449677.1"
                     /translation="LVSSPLVVGSVMVLAASIWAGDWIKQSREAARPLPAPDSPNVLL
                     VVMDTVAAEHLSLHGYPRPTSTTLVELAERGIRFDSARAASSWTLSSHATMFTGRWLH
                     ELSVGWLTPLDGTYPTLAEYLGGRGYATLGFAANNAFCARDSGLARGFTWYQDYIFPA
                     LTCFKLGALVNRPLAGIQSIEDFLERELDFYWFRPYLKYLWWLLDTTRKGAAVVNREF
                     VDWLVERRQPERPFFAFLNYYDAHTPYQLTPGRMHRFGAMAVDNSQRDLIQGWAELDK
                     SVLSTQEIEFAVNAYDECVADLDEQLGVLFDELKRQGILEHTWLIVTADHGESFGEHA
                     GVFCHGTSLYQTEVHVPLLIVPPGAIGTKKVVTETVSLRDMAATIVDVLGMTAGSPFP
                     GDSLARHWKKHLPAPDLGGAPTDQGLAEVVPNDPLNRDPSGLPKKTWPLGGLAEGEWS
                     YIRREGDVREELFHLRNDGKEQRNLADDPDARPALDRMRSQLKKLTAGPLTPERFNP"
     gene            complement(1516..2409)
                     /locus_tag="VHS97_15595"
     CDS             complement(1516..2409)
                     /locus_tag="VHS97_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3449678.1"
                     /translation="MRIVHLSDIHFWQYAFNPLRLLSKRLLGTTSLLLGRASRFRLER
                     VPELVARVQELNADHILITGDLTTTALPAEFRAALAALADLLADASRVTVIPGNHDRY
                     TGRAHYSRRFEQYFGAFAPQSAFPWLRRLGPETAILGLDPTRSGISASGKLPPAQLSA
                     AQEILAAAGPIAQLVIACHYPSAVPPRFEHEYAHKPLINRLELREWLRTIGPHLFCCG
                     HVHAAWAYRPDEIPNQLSLNPGAPLMRDSKGLCPPGFLEITLDGPTVVVHHHYWTGDS
                     WDARLLFEGGAFFGTKADLRG"
     gene            complement(2475..3302)
                     /locus_tag="VHS97_15600"
     CDS             complement(2475..3302)
                     /locus_tag="VHS97_15600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX3449679.1"
                     /translation="MGIVARSIRRRGQVAAKPGVKADDWLLERRPTSYWAATRRPFPS
                     LLLVAPLVLAYECGVLWLGKSVPGSVRTGADTWMRNGLSSLGLSDHWLLPLLLFLILL
                     TWQVLNFYDWRFSPGILAGMVLESILWAVVLLGISRLIDVGFCYLEQTGSPLLAVNAG
                     GVEPSVSSLIGYLGAGVYEETLFRLIMVPILYSALKILQIPQVLSSALAVTGSALLFA
                     LAHHAGSPGESFTWFAFIFRWMAGVFFAWVFVLRGFGIAVGSHTMYDILVGWIAWRL"
     gene            3820..5052
                     /locus_tag="VHS97_15605"
     CDS             3820..5052
                     /locus_tag="VHS97_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3449680.1"
                     /translation="MQYVENETLTNDVKGKANLYGKPELQGKVKKALASLFGDSPQHI
                     KVPQGSGLPAGGLYLGNYVQEGEGADAKLYPIYEDPTASAPLKVSDADLSLARPQEGG
                     YAIYRKNCLHCHGVSGAGDGPTSSFLYPLPRDYRKGIFKFTSTPSGAKPHRDDLRRTI
                     KNGLHGTSMPAFDALLTGPEIEQAIDYVMFLSMRGETELALIEEASISDEKDPNALSD
                     EIAREAANGIFNKWKLALTQVLNPPTPRTPSTRESVLRGRDLFLGRTTEKLECAGCHG
                     PLALGNGPSFVSQDVFNEVIFSGNPSERRLRIEKLDEKTKLLWNQKPDDWGNALRPAN
                     LNLGIYKGGRRPIDIFWRVAKGINGAQMPAHYPSPLNEQKVWDVVNFVLALPYEPELL
                     ENAGTRPAGTAVASRAPE"
     gene            5191..6012
                     /locus_tag="VHS97_15610"
     CDS             5191..6012
                     /locus_tag="VHS97_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX3449681.1"
                     /translation="MRYWSVLFGAAAVLSVLTFLYAPFSSDWWLPVAGTPTHVISTFG
                     REIDSLFIIILVITGIVFIGTQIVLVWAQYKFADELDAQGKPVRRAQYFHGSQRLEVI
                     WTIVPAAILVFIALYQMGTWAAIKFRSAAPRVPALAEITGRQFQWVMRYPGPDGKLNS
                     SDDLFTVNDLHFVKNKQALIYLKSSDVLHSFYLPILRIKQDAVPVITIPVWFDSDTAG
                     EFELVCAELCGWGHYKMRGKVTIHENEAEFQKWVDDQLVEQNRSQLTMTTGPEGR"
     gene            6018..>6309
                     /locus_tag="VHS97_15615"
     CDS             6018..>6309
                     /locus_tag="VHS97_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449682.1"
                     /translation="MSAESTSHGSSGTPDVHAGGHDHTHGDDHIHPAPSTFLTKYVFS
                     TDHKIIGIQFLFSGLIFMVLGGLLAMAVRWQLAWPWSPVPILSQIMEPQWKRS"
BASE COUNT         1276 a         1860 c         1941 g         1232 t
ORIGIN      
        1 tactagtcag ttccccgctg gtcgtcggct cggtaatggt cctggccgcg tcaatctggg
       61 caggcgactg gatcaagcaa tcgcgcgagg cagcgaggcc gttgccagcg ccggattctc
      121 ccaatgtcct tctggtcgtg atggataccg tcgcggccga acatctaagt cttcacggct
      181 atcctcgacc aaccagcacg acgttggtcg agctggcgga gcgcggaata cgatttgatt
      241 cggccagggc ggcctcatca tggacgctgt catcgcatgc gacgatgttc acggggcgat
      301 ggttacacga gctttcggtc ggctggctta cgccgctcga cggaacctat cccacgctgg
      361 ccgagtatct gggaggacgt ggctacgcga cgttgggttt cgccgccaac aacgcgtttt
      421 gcgcccgtga ttcaggcctg gcccgcggtt tcacttggta ccaggattac atctttcccg
      481 cgctcacttg cttcaagttg ggcgctctcg tgaatcgacc gctggccggg atccagtcga
      541 tcgaagattt tctcgagcgg gaactggatt tctattggtt tcggccctat ttgaagtacc
      601 tctggtggtt actggacacc actcgcaagg gggcggcggt ggtcaatcgc gagttcgttg
      661 actggctggt cgaaaggcgg caaccggagc ggccgttctt tgctttcctg aactattacg
      721 acgctcatac cccctatcag cttacgccgg ggagaatgca ccgattcggg gccatggcgg
      781 tcgacaacag ccagcgcgat ctgattcagg gctgggccga gctcgataag agcgttcttt
      841 ccacccagga aatcgaattc gccgtcaatg cctatgatga atgcgtcgcg gaccttgatg
      901 agcagctcgg cgtgctgttc gacgaactta agcgccaagg cattctcgaa cacacatggc
      961 tgatcgtcac cgcggaccat ggcgagagct tcggtgagca cgcgggcgtg ttctgtcatg
     1021 ggaccagcct ctaccagacg gaggtgcacg tgccgctatt gatcgttcct ccgggtgcga
     1081 tcggcaccaa gaaggttgtc accgagacgg tcagtcttcg cgacatggcg gcgacaatcg
     1141 tcgacgtctt gggtatgacg gccggctcgc cgtttcccgg ggattcgctg gcccggcact
     1201 ggaaaaaaca tctcccggcg ccagaccttg gtggtgctcc caccgatcag gggctagcgg
     1261 aagttgtccc caacgaccca cttaatcgcg atccgtcggg cttacccaaa aagacctggc
     1321 cgctgggcgg tctggctgag ggcgagtggt catacatccg ccgtgaaggc gacgttcgcg
     1381 aagagctgtt ccatttgcgc aacgatggga aggagcagcg caatctcgcg gacgaccccg
     1441 acgcgcggcc cgcactcgat cgcatgcgga gccaactgaa aaagctcacg gccggaccct
     1501 tgacgccgga acggttcaac ccctgagatc ggccttcgtc ccgaagaacg cgcccccctc
     1561 aaagaggagg cgagcgtccc aggaatcacc ggtccaatag tggtgatgga cgacgacggt
     1621 ggggccgtcg agcgtgatct cgaggaaacc cggcgggcac agacccttgc tgtcgcgcat
     1681 tagcggagcg ccggggttca acgagagctg gttggggatc tcgtcgggtc tgtaagccca
     1741 ggcagcatgc acgtggccgc agcagaaaag atgcgggccg atcgtgcgga gccattctct
     1801 cagctcaagt cgattgatga gcggtttatg tgcatattcg tgctcgaacc gaggggggac
     1861 ggcgctcggg taatggcagg cgattacaag ttgcgcgatt ggcccggccg ccgcgagaat
     1921 ctcttgcgca gcgctcaact gggccggggg cagcttcccg cttgccgaga tgcccgaacg
     1981 ggtcgggtcg agccccagga tcgccgtttc ggggccgagt cggcgcagcc agggaaacgc
     2041 actctggggc gcgaacgcac caaagtactg ctcaaagcgg cgcgaataat gggcgcgtcc
     2101 agtgtaacga tcgtggttcc cgggaatgac cgtaacccga ctggcgtcgg ccagcaggtc
     2161 cgccaaggcg gccagggccg cgcgaaactc agccggcagg gcggtcgtgg tcaggtcgcc
     2221 ggtgatcagg atgtggtcgg cattcaactc ttgcacgcgg gcgaccagct cggggactcg
     2281 ctccagccgg aatcgactcg cccgtcccag aaggagcgat gtcgttccca gaagacgctt
     2341 gctcagaagg cgaagcgggt tgaacgcgta ttgccagaaa tggatgtccg agaggtgaac
     2401 gatgcgcaac tggccgggtc tcgacgttta tgccggtaat gacggcgggt gtcgggccgg
     2461 atttggcctg gccgtcataa tcgccaggca atccagccca ccaagatgtc gtacatggtg
     2521 tgagagccca cggcaatccc aaagccccgg agcacgaaaa cccaggcgaa gaaaacgccg
     2581 gccatccagc ggaaaatgaa tgcaaaccag gtgaacgact cgcctgggct gccggcgtgg
     2641 tgggccaggg caaataggag cgccgagccg gtcacggcca acgcgctcga gagcacttgc
     2701 gggatctgca agattttgag ggcgctatac aggatgggca ccatgatcag gcgaaacagc
     2761 gtctcctcgt aaaccccggc gcccaggtag ccgatcaagg aagagaccga cggctctacg
     2821 ccgccggcat tcacagccag cagcgggctt ccggtctgtt ccaggtagca aaaccccacg
     2881 tcgatcaatc gacttatgcc cagaagcaca accgcccaca gtatgctttc gaggaccatg
     2941 ccggccagga ttcccggtga gaaccgccag tcgtagaagt tgagcacttg ccaagtgagc
     3001 aagatcaaga agagcaagag tgggagtagc cagtggtcgg agaggccgag cgatgacaat
     3061 ccgttgcgca tccatgtatc ggcgccggtc cgcacgctgc cgggcaccga ttttcccaac
     3121 cagaggactc cgcattcgta ggcaaggaca agcggggcga ccagcagtaa cgacggaaaa
     3181 gggcgccggg ttgccgccca gtacgacgtc ggccggcgct cgagcagcca gtcgtcggcc
     3241 ttcacgcccg gcttggccgc aacctggccg cgtcgtcgaa tcgatcgagc gacaattccc
     3301 attgtgactc tcagctagag agaagaccgg aaaatcgcga ttttagtggt ggtttccgtt
     3361 cgctcaagcg atgtcgattg caggttcttc gtaattgtta tcgtttttgt cgccgccgaa
     3421 aaatacgaaa tcactcgctg ccttgtagct ctcgggcccg acggctacga taaccctatt
     3481 cttcagctcc actcccttga cgggcctgga gttcgatttc agaatggcga cgcatttggt
     3541 cgcctcagtc gttcgccccg ccgtaatcgc aacgtttttt tgggtcgttg gcgtgctcga
     3601 ccggttattg atctcactct gtcgttgccg gggctgccag aatggcgggc tcggtcacga
     3661 tcggtttgtc gggctggagc gccgctgcgc ggtctgggtg ccacaggatg ctgggaggta
     3721 gagcgcgtca tgtcgcggtt tggatttgca tttttggtgg gctgcgcgtg cttgacaacg
     3781 tcgatgatcg gttgttcgga cgcctggaat gccggtccca tgcaatatgt cgagaacgag
     3841 acgctcacca acgacgtcaa gggcaaagcc aacctctacg gtaagccgga gcttcaaggt
     3901 aaggtcaaga aggcgctggc gtcgctcttt ggtgactcgc cccagcacat caaggttccc
     3961 caaggatcgg gattgcccgc gggggggctc tacctcggca attacgttca agagggagag
     4021 ggcgccgacg ccaagcttta ccccatctac gaagatccaa ccgcaagcgc gcccttgaag
     4081 gtcagcgatg cagacttgag cttggcccgg ccgcaggaag gaggctatgc gatttatcgc
     4141 aagaattgcc tccactgcca cggcgtctct ggcgctggcg acggcccgac gtcctcgttt
     4201 ttgtatcccc tgccccgcga ttatcgcaag gggattttca agttcacatc gaccccgagc
     4261 ggtgccaaac cgcaccgcga cgatctgcgc cggacgatca aaaacggtct tcacggaact
     4321 tcgatgccgg cgttcgacgc cctgttgacc gggcccgaga tcgagcaggc cattgattac
     4381 gtgatgttct tgagcatgag aggagagacc gagctggcct tgatcgagga agcatcgatc
     4441 tcggatgaaa aagatccgaa cgcgctatcg gacgagatcg cccgggaggc cgccaatggc
     4501 atcttcaata agtggaagct ggcactgact caggtcttga atccgccgac accccgaaca
     4561 ccttcgaccc gcgagagcgt cttgcgcggg cgcgacctgt ttctcggtcg gacaactgaa
     4621 aaactcgagt gcgccggctg ccacggacct cttgccctgg ggaacgggcc cagcttcgtc
     4681 agccaagacg tcttcaacga ggtcatcttc agcggtaacc ccagcgagcg gcggctgcga
     4741 attgaaaagc ttgatgaaaa gaccaagctc ctctggaacc aaaagccgga cgattgggga
     4801 aatgccctgc gcccggccaa tctgaatcta gggatctaca aggggggccg gcggccgatc
     4861 gacatcttct ggcgggtcgc caaggggatc aacggtgccc agatgccggc gcattacccc
     4921 tcgccattga acgagcaaaa ggtttgggac gtggtcaatt tcgtgctggc actaccatac
     4981 gagccggaac tgctcgaaaa cgccggcact cgcccagccg ggacggcagt ggcctctcgt
     5041 gcgcccgaat agtgaggcaa gaccccgagc cgagagaaga gagaaccggt tccatgactg
     5101 aatcagccgg tcggataaga gcatggtgac gctatcgcgc aatcgagtgc tgattattga
     5161 attccggaca ccttgaaagg gagcgttttc gtgcgttact ggagtgtcct ttttggcgcg
     5221 gccgccgtgc tcagcgtgct gactttcctc tacgcccctt tctcgtcgga ctggtggctc
     5281 cccgtcgccg gcacacccac ccatgtgatc tcgaccttcg gccgggaaat cgacagcctt
     5341 tttatcatca ttctcgtcat cacgggcatt gtctttatcg gcactcagat cgtcctggtc
     5401 tgggcccagt acaagttcgc ggacgagctt gacgcccagg gcaagcccgt ccgccgggct
     5461 cagtattttc acggcagtca gcggctcgag gtcatctgga caattgtccc ggcggccatc
     5521 cttgtcttca tcgccctcta ccagatggga acctgggcgg cgatcaagtt ccgcagcgcc
     5581 gctccgcgcg taccggcttt ggccgagatt accggccgcc agtttcaatg ggtgatgcgc
     5641 taccccggcc cggatggcaa gctgaattcg agcgacgacc tcttcaccgt caacgacctc
     5701 cattttgtca agaacaagca agcactcata tatctgaagt cctcggacgt tctccactcg
     5761 ttttacttgc cgatcttgcg catcaagcaa gacgcggtac cggtcatcac gattccggtc
     5821 tggtttgact ccgacacggc cggagagttt gagctggtct gcgccgagct ctgcggctgg
     5881 gggcattaca agatgcgcgg caaggtcacg attcacgaga acgaagccga atttcagaaa
     5941 tgggtcgatg atcaactggt tgagcaaaac cggagtcaac tgaccatgac caccggtcca
     6001 gaggggagat gacgaggatg agcgcagaat cgacttcaca cggatcctcc ggcacgccgg
     6061 acgttcatgc cggtggtcac gatcacaccc acggcgacga ccatatccat cccgccccgt
     6121 cgacgttctt gaccaagtat gtgttttcta ccgatcacaa gatcatcggc attcagtttc
     6181 tcttttcggg cttgatcttc atggtgttgg gcgggctcct ggcgatggcc gtgcgctggc
     6241 agttggcgtg gccctggtcg ccggtgccga tcttgagcca gatcatggag ccccagtgga
     6301 agagatcgg
//
LOCUS       DATAQL010000902         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4519620, whole genome shotgun sequence.
ACCESSION   DATAQL010000902 DATAQL010000000
VERSION     DATAQL010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4519620"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VHS97_15620"
     CDS             complement(<1..413)
                     /locus_tag="VHS97_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3449683.1"
                     /translation="MPERIFVVGHAAVTCLGQDMDSTWQGLVEGRSGIRRHASLGSDS
                     FLQDVAGIVENFGPGSTTEDHAVAKLGARFLHFSMAAARSAWTDAGLDRLEGQFDPNK
                     AAVVIGSAFGGLDLLDAEQQRMIKRKSLAVSPYLVP"
     gene            646..1500
                     /locus_tag="VHS97_15625"
     CDS             646..1500
                     /locus_tag="VHS97_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3449684.1"
                     /translation="MKFAMQDDQTSYVGVVQGDQLIPVGEGESALSDILHVGDPTAAL
                     RAVLASAERAVAISSVRVLAPIDDQEVWGAGVTYERSKVAREQESDRAATFYDLVYRA
                     DRPELFFKATRSRVVGPGQAIRVRRDSIWCVPEPELALVLSPTLELKGITIGNDVSAR
                     DIEGENPLYLPQAKVYEACCALGPSITLGAAVPPAASIGIRMEIERDGAVIYEGETSV
                     ARMARRFDELIDWLGRDNVFHAGVVLLTGTGIVPPDDFSLRPGDLVRITIDGIGVLAN
                     PVVQRTTV"
     gene            1577..2107
                     /locus_tag="VHS97_15630"
     CDS             1577..2107
                     /locus_tag="VHS97_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449685.1"
                     /translation="MDTNRVGRVPDDLMIFYFFSSFGVVTGGTAGFSPAPPGFTAAPP
                     AGGVAGVVGGASSVQPANAMARARLESIDKAWIADFTFTGALLESCVTRQIISISKSQ
                     LTHASGPLVAKTIREMTLPHPASVRIIVDRSGSPQRVVGFWDSIGTVSRHDTYWSEEL
                     VDTRSVTTGFRVVFAN"
     gene            2306..2587
                     /locus_tag="VHS97_15635"
     CDS             2306..2587
                     /locus_tag="VHS97_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX3449686.1"
                     /translation="MNLMTNLMGSERNELMAVVVDAIRSVSAKSRTTPITPASLLLEE
                     LALDSLDLVAVIVHIQDHFGVEIDPDEIPNLRTVDDVVSSLKSQLQAAA"
BASE COUNT          566 a          883 c          859 g          611 t
ORIGIN      
        1 ggaacgagat aagggctcac cgccaggctc ttgcgcttga tcatccgctg ctgttcggcg
       61 tccagcaggt cgaggccgcc aaatgccgaa ccgatgacga ccgcggcctt gttcgggtca
      121 aactgaccct cgagccggtc aagcccagca tcggtccacg ctgatcgtgc ggccgccatc
      181 gagaaatgga gaaacctcgc cccaagtttc gccacagcat gatcttccgt cgtcgaacca
      241 ggtccgaaat tctcgactat ccctgcgacg tcctgaagaa aagagtcgct acccagcgat
      301 gcatgacgtc gaattcccga tcggccttcg accaggccct gccaggttga atccatgtct
      361 tgacccaggc aggtcaccgc ggcatgaccg acgacgaaaa ttcgctccgg catcccggtc
      421 tcccagtgcg actatccaac ccgactccct gattcggcgc gccggcgccc gctgcccggc
      481 agaactgcca gcttcataat attcggcaaa gttcctacag tcacttgagc cgattcccgc
      541 tcgagccagt tgatccgcgt cagcttctcg attaacagag gagtcgacac tgaggagtta
      601 aagttcacgc ccaggacttg atcgaggcac aagacgatgc agatcatgaa gttcgcgatg
      661 caagacgacc aaacttccta tgtcggcgtg gtccagggcg accagttgat ccccgttggc
      721 gaaggcgaga gtgccttgtc ggatatcctt catgtcggtg atcccacggc agcgctgcgc
      781 gccgtgctcg cgtccgcaga acgtgcggtt gcgatttcat ccgtgcgtgt gttggcgccg
      841 atcgacgacc aggaagtttg gggcgcgggc gtcacgtatg aacgcagcaa agtcgcgcgc
      901 gaacaggaat ccgatcgcgc ggctaccttt tacgacctgg tctacagggc agaccgaccg
      961 gaactcttct tcaaggcgac tcgcagcagg gtcgttggcc cgggtcaggc gatccgagtg
     1021 cggcgcgact cgatctggtg cgttcccgag cccgagttgg ctttggtttt gagtccgaca
     1081 ctcgagctca agggcatcac gattggcaac gacgtgagcg cccgcgatat cgagggggaa
     1141 aaccccctgt acttgccgca agcaaaggtg tacgaagcgt gttgcgccct gggaccatcg
     1201 atcacgctag gggcggctgt gccgcccgcg gcatcgatcg gcatccgcat ggagatcgag
     1261 cgcgacggcg ccgtcatcta tgagggcgaa accagcgtgg cgcgcatggc tcgccggttc
     1321 gatgagctca ttgactggct cggtcgcgac aacgtttttc acgccggcgt cgttctatta
     1381 accggcactg gaatcgtgcc gcccgacgat ttcagcctga ggccgggcga cctggtgcgg
     1441 atcacgatcg acggaatcgg cgtgctcgcc aacccggtcg tccagcggac gaccgtttaa
     1501 cgaaccgcga gttagtcctc agaaaaagaa cgagccgcac cctcccaagt ggaggacgcg
     1561 gccgcgatgg ctcgaattgg acacgaaccg ggttggccgt gtgccggacg acctgatgat
     1621 cttttacttc ttctcttcct tcggtgtcgt cacgggcggc acggccggct tctcgccagc
     1681 gccaccgggc ttcaccgctg cgccgccggc gggcggcgta gcgggcgttg ttggtggtgc
     1741 ttcttcggtg cagccagcta acgccatggc cagagcgagg ctcgagagca tcgacaaggc
     1801 ttggatagca gattttacct tcacaggtgc gctccttgaa agttgcgtaa cgaggcaaat
     1861 catctcgatt tccaaatccc agttgacaca tgcttctggg ccgctcgtcg ctaagacgat
     1921 acgggagatg acattgcccc atcccgccag cgtccggatt atagtggatc gatctggctc
     1981 accgcaacgt gtcgtcggtt tttgggattc aattgggacg gtgtcccgtc acgacactta
     2041 ctggtcggaa gagttggtcg acacgcgatc ggtgacgacc ggatttcgcg tcgtctttgc
     2101 aaattgaagt caagaagcgt gctccatggt tcgatgataa tgtggtgaga cgcggcggag
     2161 tcgcggatcg tcgaattacg gatggcgttc gacggaggag acgcctgtgg cccccatggt
     2221 ccgttcgctt ggcgatcaac ttgtcgattt tgacgaaagt gccaaaaaag gcgagctctg
     2281 accttctctc caatcgaggc gtaggatgaa tctgatgacg aacttgatgg gctcagagcg
     2341 caacgagcta atggccgtcg ttgtcgacgc catccgcagc gtttccgcaa agtcgcgcac
     2401 gacgcccatc acacccgcct cgctgcttct tgaagagctg gctctcgact cacttgacct
     2461 tgttgcggtc atcgttcata tccaagacca cttcggtgtg gaaatcgacc cggacgaaat
     2521 acccaatttg cgcacggtcg atgacgtggt atccagtttg aagagccaac ttcaggctgc
     2581 ggcgtgacgt tctttacacc aacaacaggg cacggctttc atccctcgcg ctacaccgct
     2641 ttaccacacc gcatcggggc ggcgcgataa ttcggttaca ctctataccc gacgggttgg
     2701 tggttcgggc ccattccctg ctcgaatccc gtgcatccgg tcatgatccg ggttctgtct
     2761 tctcgccgct ggatcgttca tgagtgtttg ggccctgctc gttctgaccg caatgttact
     2821 catgctcccc tggatttccg agctcggcgc tagcgcaatc gggcgcgcgc cctccaatcc
     2881 ggagatccgc ggctttttgc gcgtgctggc agcgcttaa
//
LOCUS       DATAQL010000903         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2689809, whole genome shotgun sequence.
ACCESSION   DATAQL010000903 DATAQL010000000
VERSION     DATAQL010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2689809"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VHS97_15640"
     CDS             <1..1071
                     /locus_tag="VHS97_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3449687.1"
                     /translation="QISPNASRLLGRYGLGDAMARVAVRPSAIVFRRWLDGRVLGREE
                     LGDPIEDQYGAPYYHCHRADLIALLAEAFGPKDIKLGRRLVDIEQDESGVTAHFQDGA
                     SERGDLLIGADGIHSRVRERLFGEEKPRFSGQIAYRGLARAERVAHLRLPLDVTNWVG
                     PGGHFVHYYVSAGRFLNFIAVSEEATWAQEQWSDRGSISDAAKNYEGWHPQVATILTA
                     VDETFKWALFDRNPLPEWTQGRVTLLGDACHPMLPFMAQGAAQAIEDGATLAACLKNT
                     AFNVPTAFQAYVASRKPRTTILQERSQGFSKSFHLHDGPEQHARDQTYATVGLRGNPE
                     AMNSLYGHDAEGATATVTLGQK"
     gene            1684..2136
                     /locus_tag="VHS97_15645"
     CDS             1684..2136
                     /locus_tag="VHS97_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3449688.1"
                     /translation="MANVDASVDREQTMLTAVKQYFVRADAGQASVLDLFTDDVQIYF
                     PKFGIATGKAAFGELATGLLGSLKSIAHYISDFKYVVGSDSVVVEGTTHGADREGVEW
                     SGGKTAGGRFCSVFEFRGPLIARMHIYLDPDYTGRDENRFLWGRDRRW"
     gene            2153..2599
                     /locus_tag="VHS97_15650"
     CDS             2153..2599
                     /locus_tag="VHS97_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3449689.1"
                     /translation="MAAELTDEQSAMREMALEFARTKLAPFAGKWDAKKDPSARDAER
                     GRGTGMGGVTVREELGGSGLGRFDSTIIYEALATGCPRVAAFLSIHNMAVWMIDAFGS
                     RGFREQVVPNLTSMDSIASYCLTEPGSGSAPRHCGRGRFSMATLTS"
BASE COUNT          537 a          773 c          864 g          499 t
ORIGIN      
        1 cagatcagcc ctaacgcttc ccgtctgctt ggccgctacg ggcttggcga cgctatggcg
       61 cgggtcgcgg tccgtccctc cgcgatcgtc tttcgtcgct ggctagacgg tcgcgtcctg
      121 gggcgcgaag aattgggcga ccccatcgag gaccaatacg gggctcccta ctaccattgc
      181 caccgggctg acctcatcgc ccttctggcc gaagccttcg gtcccaagga catcaagctt
      241 ggacgacgtc tggtcgacat tgagcaagac gaaagcggcg tgaccgccca ctttcaggat
      301 ggtgcgtccg agcgcggcga tctccttatc ggggcggacg gaatacattc ccgcgttcgc
      361 gagcggctgt tcggcgagga aaaaccacgg ttctctgggc agatcgcgta tcgggggctt
      421 gctcgggctg aacgtgtcgc gcatctccgc ctcccgctcg atgtcacgaa ctgggtcggg
      481 ccaggcggac acttcgtcca ctattacgtt tccgccggcc gatttctaaa ctttatagcg
      541 gtcagcgaag aggcgacctg ggcgcaagaa caatggtccg accgcggttc gatatccgat
      601 gccgcaaaga actatgaagg gtggcatccg caggttgcga cgatcctcac ggcggtcgat
      661 gagacgttca agtgggcgtt gttcgatcgc aatccgctgc cggaatggac gcaggggcgc
      721 gtgacacttt tgggagacgc gtgtcatccc atgctgccgt tcatggcgca aggggcagcg
      781 caggctattg aagacggtgc gacgttggcg gcctgcctga aaaataccgc cttcaatgtt
      841 ccgacggcgt ttcaggccta cgtcgcatcg cggaaaccga gaacaaccat cttgcaggag
      901 cgatcccaag gcttttctaa gagcttccat ttgcacgatg gacccgagca acatgcgaga
      961 gatcaaacgt acgccacggt gggtctgcgc ggcaatcccg aggccatgaa tagcctttac
     1021 ggccacgatg cggagggcgc gacggcgacg gtgacgctgg ggcaaaaata acgacccgcc
     1081 atccaaagtc cctatttcga ttgcccgaca gatgacgaag gtccgacgta ttcttgagaa
     1141 gctcggccgc tcaatcgctt ccgcggagga agcaagggac attcttgtgc ccaagggaag
     1201 cgatttcgcc gtattccgaa cggtgacgga cctggggatt cctgcacacc ggccctgcaa
     1261 gggggcctct tgatttcaag gagagcacta tcaaaagttc ggatgtctcg tcatctgccc
     1321 agcggaagca ggcacaaaga aggggtcgaa ttcgctcggc tacgaaaggc aaagaggcat
     1381 catgggaacg cgtctcgtag gcaaggtcgc gatcataacc ggagcgagcc gggggcaggg
     1441 cgctgcgacg gcgaagctgt tcggccgtgg agggagtcta ttggcgaggg atcgcgagca
     1501 ttgacgtcgg cgagcatgat ctgcgcgccc tcggaagcgc atcctcgtgt gcatgaaaaa
     1561 cgcccggttc cgtttgcatg accatcgaac tgttaccacc tctggctcgc cgacagaggc
     1621 gacgcagcac aaacaccgga ctttgcatcc ggtcggtgca tatcaacttg agaggcgacc
     1681 ccaatggcca atgtagatgc atccgtcgat cgcgagcaaa ccatgctgac ggccgttaag
     1741 cagtactttg tcagggccga cgcgggccag gcaagtgtgc tcgatctctt cacggacgac
     1801 gtccagatct acttcccgaa attcggcatc gccacgggca aagcagcttt cggcgaactg
     1861 gcgacgggac tgctcgggtc gctcaagtcg atcgcgcatt acatttcgga cttcaaatac
     1921 gtcgtcggtt cggattcggt cgtggtcgaa ggcacgaccc atggcgcgga ccgcgaaggc
     1981 gtcgaatgga gcggcggaaa gacggcgggg ggaaggtttt gtagcgtgtt cgagttccgg
     2041 ggtccgttga tcgcgcgcat gcacatctac ctcgatcccg actacaccgg ccgcgacgaa
     2101 aaccggttcc tctggggtcg cgatcgccgc tggtaggcgc cggaccagag cgatggccgc
     2161 tgagctgaca gacgagcaga gcgcgatgcg cgagatggcg ctcgagttcg cgcgcaccaa
     2221 gttggcgccg tttgccggaa aatgggacgc aaaaaaagac ccttccgctc gagacgctga
     2281 aagaggccgc ggcactggga tgggcggcgt gaccgttcgg gaggaactgg gcggatcggg
     2341 acttggccgc ttcgactcaa cgatcatcta tgaagcgcta gcgaccgggt gcccgagggt
     2401 cgcggcgttt ctctcgatcc acaacatggc cgtctggatg atcgacgcat ttggctcgcg
     2461 ggggtttcga gagcaagtcg tgccgaacct gacgtcgatg gacagcattg caagctactg
     2521 cctcaccgag cccggttcgg gttcggcgcc gcggcattgc ggacgcgggc ggttctcgat
     2581 ggcgacgctt acgtcgtgaa cggcgaggag ggcttcgtgc gagtcgtcac gcgcgccgac
     2641 aacagtgtcg ttctcagggt ttacggtgcc ggc
//
LOCUS       DATAQL010000904         9715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2385363, whole genome shotgun sequence.
ACCESSION   DATAQL010000904 DATAQL010000000
VERSION     DATAQL010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385363"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="msrB"
                     /locus_tag="VHS97_15655"
     CDS             complement(<1..635)
                     /gene="msrB"
                     /locus_tag="VHS97_15655"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX3449690.1"
                     /translation="MSRFYAQLWVGVPAAAFFCLVCSLGARAQDPLANAPLAPGSAVP
                     NGASDQNKPDSTTKSETKALAKPEPEFVVKTDAEWRKILTRTQYAVTRQKATEPAFSG
                     KYATGHYKGTFLCVCCDAALFGAEAKFDSGTGWPSFDRPASMKSIKTAWDNSEIEPRV
                     EVMCRRCGAHLGHVFDDGPTLTRLRYCINSAALKLKPTDSHGALKTVSRETT"
     gene            complement(860..2107)
                     /locus_tag="VHS97_15660"
     CDS             complement(860..2107)
                     /locus_tag="VHS97_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449691.1"
                     /translation="MTKRENMKALILKEFDEDMTPEEFRANELAMKVFGLLPRDFNLK
                     DVLAQVYSEEVAAFYDPKTKTMHLIEETKTAVKQPPTLLERIFGKKDAFDKDENKTVI
                     AHELTHALADQHYDLDAMQKAVKKDDDRSLALDALIEGEATLAMFGAGMDDWDGDDIT
                     KVPAENLEFTFKLLSPFLSFLGGGKTLRSVPPIISESMIFPYFQGMVFCAKLTNEGGW
                     ASIDLVYRNPPLSTEQIIHPEKYRAKPDFPMTIDLGLLKPGEGWKEVGRNVLGEMQMA
                     VLLKKHGGKRAAAGWDGDRYAIFEGPKNRLGLVWLSTWDSEDDAREFAHAYVAYQSAK
                     VGDLGKAPRPIPDSVWRNLDDRLFVVQRRGSNVAVVEGFSPEATPGLIEAAFQARQTE
                     AKPAELKKDAAKTSSAKPLTLAN"
     gene            complement(2116..2346)
                     /locus_tag="VHS97_15665"
     CDS             complement(2116..2346)
                     /locus_tag="VHS97_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449692.1"
                     /translation="MRYFSVRVGAVLAVAATLYCAGAGRADDVAVGKGTALLAEGDRL
                     ADQGQFTEAVIRYKRAMEELLPVYGESRSNMK"
     gene            complement(2408..3727)
                     /locus_tag="VHS97_15670"
     CDS             complement(2408..3727)
                     /locus_tag="VHS97_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449693.1"
                     /translation="MKSDPDLTLETDRLEQLLETPPEPQSVVVVEYRNRGVPTWIFFP
                     LIFMVPLGAIIVYHRTVTERYRVEAAQTRQSLDNLAAKGGASAPNVGAGNAAPTPNLF
                     IVGSSDSQTSPTSAGHTQVAALASDGVDGSTVATASSAPSPGGPAVSLPLAQTGTTNS
                     ENPVAAESVAPPPDAQANRRITMRSVLPNPFADRGEAPLPPAPGEGPASRPAGPGEQT
                     STVAGQSEPGPRPPAQEQVHNDPQPVNELASRDERLAPQAPLPSKEESLRQIEEEAAK
                     LRAASLVQVENKVANQRSQRIADQIKFHDELREAIRSFGYQAGPEIEKIAKRYDGEIN
                     AGNWAKARQAWRFSRLSQSAKVKYVRDLELPEVAILEFLCSDQDPQIGKRGGPRNQSE
                     VRVRAASRLLKYAFPGTDAAPRAGGAATDAPAARTQASSTTNGAARP"
     gene            complement(4046..4858)
                     /locus_tag="VHS97_15675"
     CDS             complement(4046..4858)
                     /locus_tag="VHS97_15675"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX3449694.1"
                     /translation="METTQSPAHQALTPRYNCLVISDLHLGSDVCQAKLLEEFLIWAV
                     EHCDELVINGDVFDDLNFKRLTKRHFACLKVIRRNSDRDDFRLVWVRGNHDGPIDIIS
                     HIVGVEILDEYVYDNGRIKLLILHGDQFDTFTTAYPWLTEIACGIFYYIQKWMPHRAA
                     RWIRRISKRWQRNSQLIERRAVEYARSRGYWFVTCGHTHLPLVSRHDGLLYVNSGTWT
                     EFPPCPFVAVRGNEVRLEYWPLAEAESTETEPTVEQTAEVRPISVQETVPPA"
     gene            complement(4953..9419)
                     /locus_tag="VHS97_15680"
     CDS             complement(4953..9419)
                     /locus_tag="VHS97_15680"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3449695.1"
                     /translation="MDGSDERNDSVLDQFHPAVSSWFRRHFDEPTPAQRLGWPVIASG
                     RHALIVAPTGSGKTLAAFLAALDHLWRNHRQEKGVRILYVSPLKALNYDVWHNLQFPL
                     DGILAESQAIGALLPALRVAVRSGDTPARDRAAMVRKPPDIMITTPESLHLMLTSRAR
                     ETLRGISHVIVDEIHAVCGNKRGVFLALLLERLQAIATRRFVRIGLSATQRPLEEVAR
                     YLGGVEWTGEESSPDRAVARPVTIVDAGWRRDLDLQVIWPRRIDFTTSAGSIWPEIER
                     ELVSLVRQHRSTIIFANNRRTVEKLTRRLNELAAPDELDETDPVEADEPGSAVTDTFR
                     AHHGSISLEERRATEEALKRGEVAAVVSTASLELGIDMGAVDLVCQVESPGNVARGLQ
                     RVGRAGHIVKGTSKGRLIAKTPADLLESAALCRAMSQGQIEQLRVPKNCLDVLAQQII
                     ACVAMDQWEVPALFDLVRRAYPFHNLSAEAFESVLRLVSGRFPTPEFRDLRARIVWDR
                     IHNRLAPLPGTAQLALVGGGTIPDTGQFPVFLGDDGPRLGELDEEFVFEQRVGESFVL
                     GNSTWRIAAIEAHRVVVAKAEGQSAVMPFWKGESTARSPELGEAVGALAREITERLGD
                     PELLPWLERECRLTAPAAKHLRQFLWRQERLTGVVPDDRTILIETFVDPAGDLGLALL
                     SPFGGRLHHALKLALLGRIRHRFGLAPASLHADNGILFRLPAMDELPLDLMDGLTSEL
                     AERLVREELPETAFFGLRFRQNAARALLMPRPDPAKRTPLWLQRLRAKDLLQVARQYP
                     DFPIVLETVRECLDEDLDLPRLRSLLDAIESGSVRVVRRSGEIPSPFTSELIFLFTAA
                     HLYELDEPKRTDRKPAGSIVDEDLLEPLLRGGRLDEWLLPQAIGRVENRLRRLGRPPR
                     TADEMAEHLRLLGDLTPSEINGPMEAFLADLRGQGRALVIELPGTNEPSRWIGAEEAS
                     LYEDAFSPSAVPVAQARDSIVRRFLRTHALIGLADLTARYPIASALASDLLERWSEDG
                     KVVRVGESDAPELARWAERETLTEIRRATLAVRRGESLAVAPEVFADFLLRRQFVHPA
                     TRGEGPAFVELVLEKLQAFSAPAAVWENEILPRRVKGYRHAWLDEVLGQGAWLWRGAG
                     TARDDPRVAFFRRDFDGHPDKDMNSFELSADAQHVCAVLDRHGASFATDLARLAAIEP
                     SRARRALRELMNQGVVTNDRFDPARAGSEETLQSLSEAASARRSGLSLRPRPRRLLSV
                     RPEGRWSRLHGSACDAEARMLAWITVLLERYCILTREVVALEPLAPSWSEVAPFLSRS
                     EWRGQLRRGYFVEGLSGVQYASADAALDLAHLAAQPDADHLVLLCTIDPANLYGAGAP
                     LDIELLEGGVARLPRSPGNFLVLRAGRPVLIIESAGKRLTGLSWARQADIDSALNLLP
                     GLTRPERRVLKVETYNGIPVTDSRMASRLTELGFVRDYPGMTYYAGWAADAT"
BASE COUNT         1907 a         3033 c         2918 g         1857 t
ORIGIN      
        1 gtcgtttcgc gggaaaccgt tttcagggca ccatgagagt cggtcggttt gagcttgagg
       61 gcggccgagt tgatgcagta gcggaggcga gtaagtgtcg ggccgtcatc gaagacatgg
      121 cccaagtggg ctccgcaacg acggcacatc acttccaccc gcggctcaat ttcgctgttg
      181 tcccatgcag tcttgatgga cttcatactg gccggtcgat caaagctcgg ccaaccggtg
      241 cccgaatcga atttggcttc tgcaccgaaa agcgcagcat cgcagcagac gcaaaggaat
      301 gtgcccttgt agtgaccggt ggcgtacttt cccgaaaacg cgggctctgt cgccttttgg
      361 cgcgtgacgg catactgagt ccgcgtcagg attttccgcc attccgcatc agtcttgacc
      421 acgaactcag gctcgggctt tgccagcgcc ttggtttcgc tcttggtcgt ggaatccggc
      481 ttgttctggt cgctcgcacc attcgggaca gccgaacccg gggcgagagg cgcgttggcc
      541 agcgggtctt gcgccctggc gccgagcgaa cagacgagac agaaaaacgc ggctgccggc
      601 acacccaccc acaattgcgc gtaaaatcga gacatgctgt gttgtctccc ccattcggac
      661 cgaaggtccg gaccgatccg cttcatttca ggacactgcg acctaaatgc ctttgcacga
      721 tcctgggaag gcaagcctcc aggccagccg cggcgccatc cggctcggac ggacttcggc
      781 gagctcagtc gagccgagcc tcgccctccc ggaatcacgc aaggctgtta agcattatcc
      841 taatcctaga agggcaaaat cagttcgcaa gggtcagcgg tttcgctgag ctcgttttgg
      901 cggcgtcttt cttcaactct gccggcttgg cctcggtctg tctagcctga aaagcggctt
      961 cgatcaagcc cggtgtcgcc tcgggggaaa agccctcgac gacggcgacg ttagagccgc
     1021 gtcgctgcac gacaaataga cggtcgtcaa gattgcgcca gactgagtca ggaatgggac
     1081 ggggcgcctt gccgaggtcg cccactttcg ccgattggta ggcgacatag gcatgggcga
     1141 actcgcgggc gtcgtcttcg ctgtcccagg ttgaaagcca tacgagccca agccggtttt
     1201 tcggaccctc gaagatcgcg tagcggtcgc cgtcccagcc ggcggcggcg cgcttgccgc
     1261 catgcttctt gagcaagacc gccatttgca tctcgcccag aacgttgcgg ccaacctcct
     1321 tccaaccttc gcctggtttc aagagcccca ggtcgatcgt catggggaag tccggtttcg
     1381 cgcggtattt ctcggggtga atgatctgct cagttgagag cggcggatta cgatagacta
     1441 ggtcgatcga ggcccatccc ccctcattcg taagttttgc acagaagacc ataccctgga
     1501 agtaaggaaa aatcatcgac tcgctgatga tgggtggtac gctccgaagg gtcttgcctc
     1561 cccccaagaa cgagaggaac ggactcaaca gcttgaacgt gaattcgaga ttttccgcgg
     1621 gaactttcgt gatgtcgtct ccgtcccaat cgtccatccc ggcgccgaac atcgccagtg
     1681 tcgcctcgcc ttcgatgagc gcatcgagcg cgagcgagcg atcatcgtct ttcttgacgg
     1741 ccttttgcat ggcatcgaga tcatagtgtt gatcggcaag agcgtgcgtc agctcgtgag
     1801 caatcaccgt cttgttttcg tccttgtcga aagcgtcttt cttgccgaaa atccgctcga
     1861 gcaaagtcgg cggctgcttg acggcggtct tggtttcctc gatcaggtgc atcgtcttgg
     1921 tcttggggtc gtagaacgcg gccacttcct cggaatagac ctgagcgaga acgtctttga
     1981 gattgaagtc gcgcggcaaa agtccaaata ccttcatcgc cagctcgttg gcccggaatt
     2041 cctcgggagt catatcctca tcgaactctt tgaggataag cgccttcatg ttttcgcgct
     2101 ttgtcacgtc gcgtttcact tcatgtttga acgggattct ccgtagaccg gcaagagctc
     2161 ttccatggcg cgtttgtaac gtatcacggc ttccgtgaac tgaccctggt cggcgagccg
     2221 atcaccttct gcgagcagtg cggttccttt gccgacagcg acatcgtcgg ctcggccggc
     2281 gccggcgcaa tagagtgtgg ccgccacggc caagacggcc cccactctga ctgaaaaata
     2341 acgcatgagg cgtgtcctgt ttcatcatga aatcgggctg gaccgatcgc cggttaccag
     2401 gtttttctca gggccgtgcg gcgccattgg tggttgaaga cgcttgcgtt cgcgctgccg
     2461 gcgcgtccgt ggctgctccg ccagcacgcg gcgccgcgtc agtcccggga aacgcgtatt
     2521 tcaagagtcg ggacgccgcc cgcactcgaa cctcactttg gtttcggggg ccaccgcgtt
     2581 ttccgatctg cgggtcctgg tcgctgcaga ggaactcgag aatggcgacc tcggggagct
     2641 cgagatcacg gacatacttc actttcgccg actgcgacaa ccgcgaaaac ctccaggcct
     2701 ggcgggcttt ggcccagttt ccagcgttga tctcgccgtc ataccgcttg gcgattttct
     2761 cgatttctgg ccccgcctga tacccaaaag aacgaatggc ctcgcgaagc tcgtcgtgga
     2821 acttgatctg gtccgcgatc ctctgagaac gttgattggc caccttgttt tcgacctgga
     2881 ccagacttgc ggcccgcaat ttcgccgctt cttcttcaat ctgacgtagc gattcctctt
     2941 tgctgggaag gggcgcttgg ggtgccaagc gctcgtcacg gctggcaagt tcgttgaccg
     3001 gctgcggatc attatggact tgttcttggg ccggcggccg aggtcccggc tcagactgac
     3061 cagccactgt cgaggtctgt tctccggggc ccgcgggccg tgatgccggg ccttcaccag
     3121 gagccggcgg cagaggcgct tcgcccctgt ccgcgaacgg attcggcaga acggaccgca
     3181 tcgtgattcg gcgatttgcc tgtgcatccg gcggaggagc gactgattcc gcggcaaccg
     3241 gattttcgga attcgtcgtt cccgtttgcg ctagaggtaa tgacacggcc ggaccacccg
     3301 gcgacggggc ggaagaagcc gtggcgaccg tggacccatc cacgccgtcc gaagcgagtg
     3361 ccgccacttg agtgtggccg gccgacgtgg gactggtttg tgaatcgctt gatcccacga
     3421 tgaacaggtt aggcgtgggc gccgcgtttc cagcaccaac attgggcgca ctcgcgcccc
     3481 ccttggcggc caagttatcc aaggactgcc tggtttgggc ggcctcgacg cgatatcttt
     3541 cggtgaccgt acgatggtag acaatgatcg cgcccagcgg caccatgaag atcagcggga
     3601 aaaagatcca ggtgggaacg ccgcggttgc gatactcgac aacgacgacc gactggggtt
     3661 ccgggggggt ctcgagaagc tgctcgagcc gatcggtctc cagggtgaga tccgggtcac
     3721 tcttcatctc gactcacctc cgaggtcgcc attccaggtt ctattcgtgt agagaccacg
     3781 gtcatttcag ggaacaggcc aattctgagc cgcggtttgc actcgccggt ttacgaacat
     3841 gcgatccagc gccgcccgct tgacgttgca tcttaccaga ccaatcttgc ttatgcacga
     3901 tcagacagtt cgacgtcgcg atcgacttta gcgagttgaa ttcacgcaac acgcgatgcg
     3961 ccgaccaacc aacgttgtac cctcggaatt catggcgacc gctttcccat acccgggccg
     4021 agccgcgtcg ctaacccatt gcgggttacg caggcgggac agtctcttgc acagagatcg
     4081 gccgtacctc ggcagtctgc tcgacggtgg gctcggtttc tgtagactca gcctcggcta
     4141 acggccagta ctccaatcga acttcgttgc cgcgaaccgc cacgaatggg cagggcggaa
     4201 actcggtcca tgtcccactg ttcacataga gcaggccgtc gtggcgggaa accaagggga
     4261 gatgggtatg gccgcaggtg acgaaccaat agcccctgct tcgagcatac tcgacagcac
     4321 ggcgctcgat gagctggcta tttcgctgcc accgcttcga gatacggcga atccatcggg
     4381 ctgcccgatg aggcatccat ttctgaatgt agtagaaaat cccgcacgca atctccgtca
     4441 gccagggata ggctgtcgtg aatgtgtcga actggtcgcc gtgcaggatc aagagcttga
     4501 tccgcccgtt gtcatagacg tattcgtcga ggatttccac accgacgatg tgactgatga
     4561 tgtcgatcgg tccgtcatga ttgccgcgga cccagaccaa ccgaaaatcg tcgcggtcgg
     4621 aattgcgcct gatgactttc agacaggcga aatgccgctt ggtcagccgt ttgaaattga
     4681 ggtcgtcgaa aacatcgccg ttgatgacga gttcgtcgca atgctcgacc gcccagatca
     4741 ggaactcttc aagcagctta gcctggcaga cgtcgctgcc caagtgcagg tcgctgatca
     4801 cgaggcagtt gtagcgaggg gttagggcct gatgagcagg actctgggtc gtctccatcg
     4861 ctcagctccg gggtttgcat cgtgcgtcgc gaaaatccta aaagaaccgt gccgggaagt
     4921 cacggggctg tcgctccacc agacgatcaa aatcacgtcg catccgcggc ccacccggcg
     4981 taataagtca tgccaggata atcgcgcacg aagccaagtt cggtcaaacg cgaggccatc
     5041 ctgctgtccg taacgggaat cccattatac gtttctacct taagaactcg cctctcagga
     5101 cgggttaagc ctggaagaag attcagtgcg gaatcgatgt cggcctgacg cgcccacgag
     5161 aggcctgtca atcgttttcc ggcggattcg atgatcaaga cggggcggcc ggcccgcagg
     5221 accaggaaat tgccaggcga gcggggcaag cgcgcgacgc ctccctcgag caattcgatg
     5281 tcaagcggtg ctccggcacc ataaaggttt gccggatcga tcgtacaaag gagcaccaga
     5341 tgatccgcgt ctggctgggc cgccaggtgc gccagatcca atgcggcgtc tgccgacgca
     5401 tactgcacgc ccgagagccc ttcgacaaaa taacctcggc gtagctgacc tctccattcc
     5461 gagcgtgaca ggaaaggggc gacctcactc cacgacggcg ccaatggctc cagagcgacg
     5521 acttctcgcg tgagaataca gtaacgctcg agcaagacgg tgatccaggc gagcatccga
     5581 gcttccgcat cgcacgctga accatgaaga cgcgaccaac gcccctcggg acgaacggac
     5641 aacaaccgcc ggggccgggg gcggagcgag aggcccgacc ggcgcgcgct ggcggcttcc
     5701 gaaagcgatt ggagagtttc ttccgatccc gcgcgggctg ggtcgaagcg gtcgttggtc
     5761 accacccctt ggttcatcag ttctcgcagc gcccgccgtg cccgtgaggg ctcgatcgcc
     5821 gccagccgtg ccagatcggt cgcgaaactg gcgccgtgtc ggtcgagaac agcgcacacg
     5881 tgctgcgcgt ccgccgagag ctcgaacgaa ttcatgtctt tatcgggatg gccgtcgaag
     5941 tcacggcgga agaatgcaac gcgcggatcg tcacgtgcgg tacccgcgcc gcgccatagc
     6001 cacgccccct ggccaagcac ctcatcgagc caggcgtgcc ggtaaccctt gactcgccga
     6061 ggcaggatct cattctccca gacggccgcc ggagccgaaa acgcctggag cttttccagc
     6121 accagttcga cgaacgccgg cccttctccg cgtgtggcgg gatgaacgaa ttggcggcgc
     6181 agcaggaaat cggcgaacac ttcaggggcc acggccaggc tttcgccacg ccggacggcc
     6241 agggtcgccc gccgtatctc agtcagggtt tcccgttccg cccagcgtgc cagctccggt
     6301 gcgtccgact cgccgacgcg gaccacctta ccgtcctcgg accatcgttc gagaaggtca
     6361 gacgctaggg cggatgcgat cggatagcgc gccgtcaaat cggccaggcc gatcagggcg
     6421 tgggtccgta agaaccggcg gacgatcgag tctcgcgcct gggccaccgg taccgctgac
     6481 ggtgaaaacg cgtcttcata cagcgatgct tcctcggctc cgatccaccg cgaaggctcg
     6541 ttggttccgg gtagctcgat cacaagcgct cggccctggc cgcgcaagtc cgccagaaac
     6601 gcctccatgg gtccgttaat ttcggacggc gtcagatcgc ccaagagccg caaatgttcg
     6661 gccatttcgt cggccgttcg cggcggccga cccagccggc ggagccgatt ctcgacccgc
     6721 ccgatcgcct ggggaagaag ccactcgtcc aggcgacctc cacgcaagag cggctcgagt
     6781 agatcttcat caacaatcga gccggcaggc ttgcggtcgg tccgcttcgg ctcatccaat
     6841 tcgtaaagat gagcggccgt gaacaggaag atcaattcgg atgtgaatgg cgacggaatc
     6901 tcgccggacc ggcgaaccac gcggaccgat cctgattcga tcgcgtccaa gagcgatcgc
     6961 agcctcggca aatcgagatc ttcgtcgaga cactcgcgca cggtttcgag cacgataggg
     7021 aaatcgggat actgccgcgc gacttgcaag aggtctttgg cgcggagccg ctgcagccaa
     7081 agcggcgtgc gtttggccgg atcggggcgg ggcatcaaga gcgcccgcgc ggcgttctgg
     7141 cggaagcgca aaccaaaaaa agccgtttca ggcagctctt ctcggactag ccgctcggcc
     7201 agctcgctcg tcaatccgtc catcaggtcc agggggagct cgtccatggc cggcagtcgg
     7261 aagagaattc cgttatcggc gtgcaacgag gccggcgcga gaccgaaacg atggcggatc
     7321 ctgcccaaaa gggcaagttt gagcgcgtgg tgcagcctac cgccaaacgg cgaaagcaac
     7381 gccaggccga ggtcgccggc cgggtcgacg aacgtctcga tcaagatcgt ccgatcgtcg
     7441 ggtacaaccc ccgtgagccg ctcttggcgc cagagaaatt gccggaggtg tttggccgcg
     7501 ggcgcggtca gccggcattc gcgttccagc cacggcagca attccgggtc gcccaaacgc
     7561 tccgtgattt cgcgcgcaag cgcacctacc gcttccccga gctcgggcga gcgcgcggta
     7621 ctctctccct tccagaacgg catgacggcc gattgtccct ctgccttggc caccaccacg
     7681 cgatgagcct cgatcgccgc gatccgccag gtggaattgc ccagcacaaa cgattcaccc
     7741 acgcgctgct cgaacacaaa ctcctcgtcg agctcaccca gccgggggcc atcgtccccc
     7801 agaaaaacgg ggaactgacc ggtatccggt atcgtgccgc caccgaccaa ggcaagctga
     7861 gcggtgcccg gcagaggggc tagacggttg tgaattcgat cccagacgat gcgcgcgcgc
     7921 aaatcgcgaa actcaggcgt gggaaatcgc cccgacacca agcgcaaaac gctttcgaaa
     7981 gcttcggccg agaggttgtg aaaaggatag gcacggcgga ccaagtcgaa gagcgcgggc
     8041 acctcccact ggtccatcgc cacgcacgca atgatctgct gggcgaggac gtcgagacag
     8101 ttcttgggca cgcgcagttg ctcgatctgg ccctgactca tcgcgcgaca aagcgcagcc
     8161 gattcgagca ggtcggcggg cgtcttggct atcaggcgcc ctttgctggt ccccttgacg
     8221 atatgacccg ctcgccccac gcgttgcagc ccccgcgcca cattgccggg tgactcgacc
     8281 tggcagacga gatcgaccgc ccccatgtcg attccgagct cgagtgacgc tgtcgacaca
     8341 acagcagcga cctctccccg tttgagcgcc tcttccgtcg ccctccgctc ttccaggctg
     8401 atgctgccat ggtgggctcg aaacgtatcc gtaaccgccg agccgggctc gtcggcctcg
     8461 acgggatcgg tctcatccaa ttcgtctggc gctgccagct cgttgagccg cctcgtcagc
     8521 ttctcgacgg tccgccgatt gttggcgaag atgatcgtcg agcgatgctg cctgaccagg
     8581 gacacgagct ctctctcgat ttcgggccag atcgatcccg cgctggtggt gaagtcgatc
     8641 cggcgcggcc agataacctg cagatccagg tcgcggcgcc acccggcatc gacgatggtc
     8701 accggtcgag ccaccgcgcg atccggagat gattcctcgc cggtccactc cactccaccc
     8761 agataccgcg cgacttcttc caacggccgc tgtgtcgccg acaggccgat ccgtacaaag
     8821 cgcctcgtag cgatcgcctg caaccgttcg agaagcagag cgaggaagac ccctcgcttg
     8881 ttgccgcaga ccgcgtggat ttcgtcgacg atcacatgag agatcccgcg cagagtctcc
     8941 cgcgcccggc tcgtcagcat caggtgcaac gattcgggcg tagtgatcat gatatcaggc
     9001 ggcttacgga ccatggcggc ccggtcgcgc gctggtgtat caccgctgcg aaccgcgacc
     9061 ctcaaggccg gcaaaagagc cccgatcgcc tgcgactccg cgaggatgcc atccagggga
     9121 aattggagat tgtgccaaac atcataattc aaggccttca agggagatac ataaaggatc
     9181 cgcactccct tctcttgccg gtgattccgc cagagatgat caagcgcagc cagaaacgca
     9241 gccagggtct ttcccgagcc cgtcggcgcg acgatcaacg cgtgacgacc cgacgcaatt
     9301 accggccagc ctagtcgttg tgcaggcgtc ggctcgtcaa agtgacggcg aaaccacgac
     9361 gaaaccgccg ggtgaaactg gtcgagaaca ctgtcattgc gttcgtctga tccgtccatt
     9421 agtacagaaa aacccgagcg agacatctgt ggtggtcgtg cggagcacca ctcaattctc
     9481 gcgttgcggg cgccgtctcg caacccgttt tctgtacaaa ggtttatttg gagctctgca
     9541 tcacgcaaca gaagggcgat gcaacaaggc gaccggcgag tggaagctta cccaggtagg
     9601 gtgggcaccg cccacaggcc caaggtcccg ttcggtgggc ggtgcccacc ctacgagacc
     9661 cggtacagta attcttaccg ttggcccaaa gtcgactgac aaatctgtca aagga
//
LOCUS       DATAQL010000905         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2385365, whole genome shotgun sequence.
ACCESSION   DATAQL010000905 DATAQL010000000
VERSION     DATAQL010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385365"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VHS97_15685"
     CDS             complement(<1..540)
                     /locus_tag="VHS97_15685"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449696.1"
                     /translation="MRWQSTRASHRRAFTLVDLLVVLAVIAVLIALLLPAVQSAREAA
                     RRVQCINNLKQIAVAAHNYVGANGVFPQGIQFQVNPLYPPERHCYTSGSWFVAMSSYL
                     EHDAVFNAVNFDVNIYTPQNTTVSGLGQSVLWCPSDPVIARLNFTFPAGRVTATPLTM
                     YYSSYAGNSGQFFEYYAPRW"
     gene            complement(618..1232)
                     /locus_tag="VHS97_15690"
     CDS             complement(618..1232)
                     /locus_tag="VHS97_15690"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3449697.1"
                     /translation="MPEITPDSAETCRLLGEIQAGDRSAFERLFARYRPYLHQVVEAR
                     LDPKILARVDPSDVVQEAQMEVYRRLPDYLARRPMPFRLWLRKTAQERMLSIRRRNLE
                     AKGRALGREVPLPERSSLMLAKPFLAAELTPSQELDQRELIHRVHKAVARLSEADQEI
                     LLLRTFEGLSNQEVACLLDLDPQTASKRYGRAVLRLQRLLGAEL"
BASE COUNT          322 a          511 c          531 g          270 t
ORIGIN      
        1 ccatcggggg gcatagtact cgaagaattg acccgaattg ccggcgtacg agctgtaata
       61 catcgtcaag ggcgtcgccg tcaccctccc cgcgggaaaa gtgaaattga gccgcgcgat
      121 cacgggatcg ctcggacacc agaggaccga ttggcccaac cctgagaccg tggtgttctg
      181 cggcgtgtag atattgacat cgaagttcac cgcgttgaag acggcgtcgt gctcgagata
      241 gctcgacatc gcgacgaacc aactgccgct ggtgtagcag tgccgctcgg gcggatacaa
      301 cgggttgacc tgaaattgga tcccctgggg gaacacgccg ttcgcaccga cgtaattgtg
      361 cgccgccaca gcgatttgct tcaggttgtt gatgcactgc acccggcgcg cggcctctcg
      421 ggccgattgc accgccggca agagcagggc gatcaaaacc gcgatgaccg cgaggacgac
      481 caacaagtcg acgagggtga atgcgcgacg atggctcgca cgagtgctct gccacctcat
      541 gatcggcctc ctggaatcgg aacggatcgg acgaagggac cacgatgaca cacgcggcca
      601 ccatggacag atgaagacta gagttcagcc cctaagagcc gttggagccg gagtaccgcc
      661 cggccatacc gcttgctggc ggtctgcggg tcgaggtcca gcaggcacgc cacctcctgg
      721 ttcgacagcc cctcgaacgt gcgcaggagc aggatctcct gatcggcctc ggacagccgg
      781 gccaccgcct tgtggacgcg gtgaatcagc tcgcgctgat cgagttcctg gctgggtgtc
      841 agctcagcgg ccaggaacgg tttggcgagc atgagcgacg atcgctccgg gagcggcacc
      901 tcgcgcccca aggctcgtcc cttggcttcc aaattccgtc gtcggatgga gagcatgcgt
      961 tcctgggccg tcttgcgcag ccagaggcgg aacggcatgg gccggcgagc cagatagtcg
     1021 ggcaggcggc ggtagacctc catctgggct tcctggacga cgtcggaggg gtcgacccga
     1081 gcgaggatct tggggtcgag ccgcgcctcg acaacctggt gcaagtaagg ccgataacgg
     1141 gcgaagaggc gctcgaaagc cgaccggtcg ccggcctgga tctcccccag caggcggcat
     1201 gtctccgcgg aatcgggtgt gatctcgggc atgaagtcct cctacccaat tacctgcacg
     1261 aacccgccga gatggactgg gaaaatcgaa aaacccggaa tcgagccgaa tttggccggc
     1321 atcgccgcgg cagacatcgc ccgatccgcg gcgctattgt cggtcgaaag agaggccact
     1381 tcaagatatc tggagcgaat ggacccaact ggtcgtaccc aggcggtgag gcgccgccga
     1441 gttggctcct ggggcttagc gcgtcctagc tcagtgcctc ccgaagtgtg agcatggccg
     1501 gcatccttgc gcgcttcggt cggatcgacc gacgcatcgg cgagcctccc tgcgcgcgtc
     1561 ggtgtcgtcg gaccgacgcg cgcagagccc cgctctgcaa actcccttga atccaaaaac
     1621 tagccgcgtc cccg
//
LOCUS       DATAQL010000906         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3300425, whole genome shotgun sequence.
ACCESSION   DATAQL010000906 DATAQL010000000
VERSION     DATAQL010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300425"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VHS97_15695"
     CDS             <1..651
                     /locus_tag="VHS97_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449698.1"
                     /translation="CEEGLRSLVKAGLALSRFSLSAIDLLRSAMLSHALKDGFDQFLF
                     IDADIGFDPRDAIRLFNRPEPVIAGVYVHNKGRDFAGTFARGVTKVVFGPAAPGLYPM
                     LYAPTGFLRIRAEVLRRMIDELTLPECHWGSIKGIYPFLLPLCVPDGRGGTRYLTEDY
                     SFSHRLAQIGITPMADTTIKLSHYKKYGYSYEDLKPRQPVANITVEITYAEGSDHP"
     gene            842..1297
                     /locus_tag="VHS97_15700"
     CDS             842..1297
                     /locus_tag="VHS97_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HEX3449699.1"
                     /translation="MNVKRPLTPTITIGDQPTEADLTELKQEGYTAVVNLRNDGEPDQ
                     PLSTAAEGDKVRELGMDYFHHGVGSRSLTVQGVKEVCDFIDKHSQGTERVLVHCRRGP
                     RAIAILLLQQARIQQWTNAEVFAKGKAMGLELDGGLKLMVDQFLQDSTG"
     gene            complement(1313..>1689)
                     /locus_tag="VHS97_15705"
     CDS             complement(1313..>1689)
                     /locus_tag="VHS97_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449700.1"
                     /translation="AIQEFTELIRLDPQNALAHQTLARILATCNEENSRDGRRAVDEA
                     TLACELTRWRDPDCLDTLAAAYAEVGDFAAAIKWQSQAITLIRQNVPSLLHQKAASFG
                     GYRGIGFEDRLVFYKSKKPTRE"
BASE COUNT          353 a          495 c          503 g          338 t
ORIGIN      
        1 tgcgaggagg gcttgcgctc gttagtcaag gcgggattgg cgcttagccg cttctcgttg
       61 tcagccatcg acctgctgag aagtgcgatg ctttctcacg cgctcaaaga cggcttcgac
      121 cagtttcttt tcatcgatgc cgacatcggc tttgatccgc gcgatgcaat ccggctcttc
      181 aacaggcccg agcccgtcat cgcgggggtc tatgtccaca acaagggacg cgattttgca
      241 ggaacgttcg cccgcggagt cacgaaagtc gttttcggcc cagcggcccc tggactttat
      301 cccatgctct acgcaccgac cggattcttg cgcatccgag ccgaggtttt gcggcggatg
      361 atcgacgagc tcacgcttcc ggaatgccat tggggatcga tcaaggggat ttatccgttc
      421 ttactgccgc tgtgcgtccc cgatggacgc ggcggaactc gttacctcac cgaggactac
      481 agcttctctc accggctggc gcagatcggg atcactccga tggccgacac gacgatcaaa
      541 ctgtcccatt acaaaaagta tggatacagc tatgaagacc tgaagccgcg acagcccgtc
      601 gccaacatca cggtcgaaat cacttatgca gaaggcagtg atcatccgta ggggccgccc
      661 gcatgaccga cgttcgcttg gcgttgagac tggtcgatct gaatcaactt cgatcgccgc
      721 ggcggtatta gaccgtcata ttggcgatac cggtgcgggt gagttagtct cttggaagag
      781 aagatgatcc tcgttcgcgg cgaacgaggc tggcgccgcg ccctcgtctc gggagagacc
      841 catgaacgtc aaacgtccac tcacaccaac aatcacgatt ggcgatcaac cgactgaggc
      901 cgacctcacc gagctgaagc aagaaggcta tacggccgtg gtcaacttgc gcaacgacgg
      961 cgagcccgat caaccgctca gcaccgctgc cgagggggac aaagtccgcg agctggggat
     1021 ggattacttc caccacggcg tcgggtcgcg atccttgact gtgcaggggg tgaaggaggt
     1081 ctgcgacttc atcgacaagc attcacaagg aacggagaga gtgctcgtcc attgtcgacg
     1141 agggcctcgc gccatcgcga tcttgttgct gcagcaggct cgaatccagc aatggaccaa
     1201 cgccgaggtc ttcgccaagg gcaaggcgat gggactggag ctcgacggcg ggctcaagct
     1261 gatggtcgat caatttctgc aagattcgac tggctgaaag cgaaattcgc gatcattccc
     1321 gcgtcggctt cttgctcttg taaaagacca ggcgatcctc gaatccgatc ccgcggtaac
     1381 cgccgaaact ggctgctttc tgatgaagga gcgacggcac gttttggcgg atgagcgtga
     1441 tcgcctgcga ctgccacttg atggcggcgg cgaagtcacc gacctcagca taggcagccg
     1501 caagggtgtc gaggcaatcg gggtcacgcc agcgcgtaag ttcgcacgcc aacgtcgcct
     1561 catcaacagc ccgcctgccg tcacgggaat tctcctcgtt gcaggttgcc aggatgcgtg
     1621 cgagcgtctg gtgtgccaga gcattctggg gatcgagccg gatcaactcg gtaaattcct
     1681 ggatagccc
//
LOCUS       DATAQL010000907         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4825809, whole genome shotgun sequence.
ACCESSION   DATAQL010000907 DATAQL010000000
VERSION     DATAQL010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4825809"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VHS97_15710"
     CDS             complement(<1..441)
                     /locus_tag="VHS97_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449701.1"
                     /translation="MKFSAPRRPRRFRPEIITLEHRSLLTAVVTSLGQDGHDLVGPDA
                     SQGSDGIQDIHIQISGLAASVNQIVVQGPAGFEWATAPNPSGNALAEYFPSSTAGQGD
                     LYINPQVKSDLPPTGSTLPLGGSTGSLIQLQDGASLSFTITLAGQ"
     gene            complement(686..2104)
                     /locus_tag="VHS97_15715"
     CDS             complement(686..2104)
                     /locus_tag="VHS97_15715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449702.1"
                     /translation="MNRSIEAPQVPRWAWGVLAIFFAMQMLDSVANWLLAAVLPQVSQ
                     ELNLSESQAGWLSTVWLLGLAAASPPVGYLADRIRRPRLLSMGFAVWSVATVGTGLTR
                     SYDQLQLARAFAGIGGAVFAVVALTMLLDLFPRSTRSWALAAFFLAVPLGAAMGFIVG
                     AAFARATAWQTAFLAVGAPGLLLALLALVLPDPVRGSSEGVDIERLRLHERVGPSRED
                     YVDLMVNSSYTYSVFGITFSSFVFVGLLYWSGTFLVVVKGFTDFQAGGPLGLTWLGAA
                     IMGTAAGGWLAHWSTKSNPRALFVVPGLLMFAAIPFVLAAIYGRGVPWILGGLFLAQG
                     VIFMNVVPCYTIIATVVLPNMRAVACGAALASIHLLGDIWSPALMGWVVDTFGEADST
                     ATIFGRALTALGAVPVAEPGRDPENLTAGMLVVIPALLISGIVLLAGSRHLPREMALM
                     LAKLRAIPSRRLPVGPVKGPRT"
     gene            complement(2289..>2502)
                     /locus_tag="VHS97_15720"
     CDS             complement(2289..>2502)
                     /locus_tag="VHS97_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449703.1"
                     /translation="GIVRNETPDAVEIQDADAKLVRLAKDQIEERKRSDVSLMPNGLA
                     QGLSPQDFADLIAYLETLKIADAKTK"
BASE COUNT          583 a          781 c          713 g          425 t
ORIGIN      
        1 ttgccccgcc aaagtgatcg tgaaactgag tgacgcaccg tcctgcaact ggatcaaact
       61 tcccgtcgaa ccgcccagtg gaagcgtgga acctgtaggg ggcaagtcgc tcttgacctg
      121 cggattgata tagaggtcgc cctgccccgc agttgacgac ggaaagtatt ccgcgagggc
      181 atttccggac ggattcgggg ctgttgccca ctcgaagccc gccggcccct ggacgacgat
      241 ctgattgacc gaggcggcga gacctgatat ctgaatatgg atatcttgaa ttccatcaga
      301 gccctgtgac gcatcggggc cgaccagatc gtggccatcc tggcccaagg acgtcaccac
      361 agcggtcaag agactgcggt gttcaagggt aatgatctcg ggccgaaagc gacgcggacg
      421 tctcggtgcg gaaaacttca tgtgagctct tcgagattcg cggtgatacg tccctgaaaa
      481 ggggtccgtc gaatccctcc gaagaactca tagcacaatc cctacggtca catgaccaat
      541 ttgaaggatt ttgttccgga aacaagacat cttggccgcg aagtcccttt gattaacccg
      601 cgcaaacgat atggccgtca gtgtcgttct gtcctgagca ttttgggtga gcgatcaggt
      661 taccgggcgc acgattcgag tgttgtcaag tgcgggggcc tttaacagga ccaacaggca
      721 accgccggct gggtatcgcc ctcaatttgg cgagcatgag tgccatctcg cgtggcaaat
      781 ggcgcgagcc cgccagcaag acgattccgg aaatcaggag ggcggggatc acgacgagca
      841 tgccggccgt caggttttct ggatcacgac cgggctcggc tacaggaacc gcgcccagcg
      901 ccgtcagtgc cctaccgaag atcgtggccg tggaatcagc ctcgccgaat gtatcgacaa
      961 cccagcccat caatgccggg gaccagatgt cccccaagag atggatcgac gctaaggccg
     1021 cgccgcacgc gaccgcccgc atgttgggca acacaactgt tgcaatgatc gtatagcagg
     1081 gcacgacgtt catgaaaatc acgccttggg ccaggaacaa tccgcccaga atccaaggaa
     1141 cgccccgccc atagatcgcc gccagcacga atggaatcgc ggcaaacatc aagagtcccg
     1201 gcacaacaaa gagcgcccgc gggtttgact tcgtcgacca gtgggcgagc cagccccccg
     1261 cggctgttcc catgattgcc gctccgagcc atgtcaatcc cagcggtccg ccagcctgga
     1321 aatcggtaaa ccctttgacg acgaccagga aagttcccga ccaatagagc aatccgacga
     1381 acacaaacga cgaaaacgtg atgccgaaga cagagtaggt gtaagaagaa ttcaccatca
     1441 aatcgacgta atcctcgcgg ctggggccga cgcgctcgtg caatcgcagc cgttcgatat
     1501 cgacaccctc actcgagcca cggacagggt cgggcaggac gagtgccaag agagccagca
     1561 acaaaccagg cgcgccgacc gccagaaacg ccgtctgcca ggcggtcgcc cgagcaaaag
     1621 cagcacccac gataaatccc attgcggctc ccaacggcac ggccaggaaa aaggccgcga
     1681 gcgcccacga gcgagtgctt cgaggaaaca agtccaagag catcgtgagc gcgacaaccg
     1741 caaagaccgc gcctccaatc cccgcgaacg cgcgggcaag ctggagctga tcgtacgacc
     1801 gagtcagccc cgtgccgacc gtcgccacgc tccagacggc gaatcccatg gacagcaatc
     1861 gaggacgcct tatccgatca gccagataac cgacgggcgg actcgccgcg gccagcccga
     1921 gtagccacac ggtcgaaagc caacccgcct gactctcgga aagattgagc tcctggctga
     1981 cctgaggcaa caccgccgcc agcagccagt tggcaaccga gtcgagcatc tgcatcgcaa
     2041 agaagatcgc caggacaccc cacgcccaac gagggacttg tggtgcctcg atcgaacgat
     2101 tcatcgtctg attcgccgtg tctggtcctg acaccaatcc cgaacctgga ctgccgatcg
     2161 atgagtcaat catttctcaa gccccacaaa atagccgacc tcgggcgccg cgagcatcac
     2221 gatggttgcg tatttcatga ggtcgctctc ccgcagaagg ggcatgcccg gccttgatcg
     2281 caccggcttc atttggtttt ggcgtctgca atcttcagcg tctcgagata ggcgatcaag
     2341 tccgcaaaat cttgcgggct caaaccctgg gccagaccgt tgggcatcag cgaaacgtcg
     2401 ctccgcttgc gctcttcgat ctgatctttg gcgagccgga ctagcttcgc atcggcgtct
     2461 tggatctcga cggcatcggg ggtctcattg cgtacgatgc cg
//
LOCUS       DATAQL010000908         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_397609, whole genome shotgun sequence.
ACCESSION   DATAQL010000908 DATAQL010000000
VERSION     DATAQL010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397609"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VHS97_15725"
     CDS             complement(<1..590)
                     /locus_tag="VHS97_15725"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3449704.1"
                     /translation="MIDLARATARTIAQSVRRRELTAVAVIQAALERITRLNDTLHSF
                     ITVTPDQALVAAEEVDRQIAAGQGDGLPLAGVPMAIKDSFWTKGVRTTGGTKVLADFV
                     PGEDATAVARLKRAGCVLVGKSAMHEMAYGFTSRNPHYGDCRNPWDTSRITGGSSGGN
                     AAALATGMALAAVGGDTGGSNRQPAALCGVVGVKVT"
     gene            complement(587..796)
                     /locus_tag="VHS97_15730"
     CDS             complement(587..796)
                     /locus_tag="VHS97_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449705.1"
                     /translation="MPEFPDPVIDVETIRRIAGEDVMLPLTPAELDAIHSVLNPLIGE
                     IRQVTGRDRAGAEPEVIVVVEEWPA"
     gene            1680..2654
                     /locus_tag="VHS97_15735"
     CDS             1680..2654
                     /locus_tag="VHS97_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449706.1"
                     /translation="MKSQRRGFTLIELLVVIAIIAVLMSLLLPAVQSAREAARRSQCV
                     CNLKQVSLAIHGYHDTHSVLPPGKKGCCWGTWLVYTLPYLEQKPLYNAWNSFGTNVAG
                     APSNYDLDLRYFGVANQTVTSTFISTYLCPTDQTNTPITATTNGKTYACTSQNYAVNF
                     GNTVVIQTDFQDIRFGGAPFVDIGSPNGDHDQTGRATVGFNTFADGLSNTMLASEVVV
                     GQGQDLRGFSWWGDAATFEAFLAPNSSFPDVMFSPFYCINQPPNPPCIGTTTALPDNY
                     GARSRHPGGLNVAMGDGSVRFVKNSVDIRTWRAMSTTRGGEVASADQY"
     gene            2729..3538
                     /locus_tag="VHS97_15740"
     CDS             2729..3538
                     /locus_tag="VHS97_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX3449707.1"
                     /translation="MTEIGGDRQHSSGVYSERVPAIDRLAPSGRPSQRAVMRQKWRDL
                     LFVHWPIAPEALRPLVPPQLELDLFDGRAYAGLVAFTMTGVRPVGLPPVPGLSSFHET
                     NVRTYVRLADRDPGVWFFNLEAASKIAVRLARWSFHLPYHYAHMFLEHEPTLRADEPN
                     SIVYAGARHWPGPLPASYIIRARPDSPIQPAQPGSLEHFLVERYILFTVRKSRLYQGR
                     VHHTPYPLQPARVLSLDETLLAANGIDRPDTPPLAHFARGVDVEVFPLIRS"
     gene            4045..4752
                     /locus_tag="VHS97_15745"
     CDS             4045..4752
                     /locus_tag="VHS97_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX3449708.1"
                     /translation="MNTVVRLWFGCAAFSAVAMTAQAQTYYYYPTTVAAAPSVVTGSN
                     FVPTTYVGGAVGNKVTWYTYPAQSNQTAYTVQNPQTNVAPQYYQPMYQAQSVQAPSVN
                     QVAPTNNTTSNAAPATTSNAAPAAAVTASAEAGDPYGFLGWLNATRASYGLPAVGYDP
                     NLSGWAAMNNDQQAARGMGHFVMGPARRQNSAMGAFANIGSMWMNSPAHRAALLDPTI
                     RAIGIAGLGAYWTFNAY"
     gene            complement(4814..>5317)
                     /locus_tag="VHS97_15750"
     CDS             complement(4814..>5317)
                     /locus_tag="VHS97_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449709.1"
                     /translation="QRIFAPRPVAANPLPVPSADFETLWNKTVAVVDKYFDIESENRL
                     ARTIKTQPQMGATLLEPWAFDSVKIEDRIEASLQTIRRVAIVHIDPAPAGGFLVKVEV
                     YKFLEDMIKPDRQAAGRAVFTNDFPVNRTREIVGPVPAPVGWIQRGRDLDLEQAILAG
                     IRDALFL"
BASE COUNT         1061 a         1670 c         1513 g         1073 t
ORIGIN      
        1 taggtaactt tgacgccgac gacaccgcag agcgccgcgg gctggcggtt tgagcctcca
       61 gtgtcgccgc ctacagccgc cagcgccatc ccggtggcga gcgccgcggc gttgccgcca
      121 ctgctgccgc cagtaatccg agacgtgtcc cagggattgc ggcagtcgcc gtaatggggg
      181 ttgcggctgg taaatccgta agccatttca tgcatcgccg acttgccgac cagcacgcaa
      241 ccggcccgct tgagccgagc aacggcggtg gcatcctcgc caggtacgaa gtccgcgaga
      301 actttcgtgc cccctgtcgt gcgaacgccc ttggtccaaa agctgtcctt gattgccatc
      361 gggacaccgg ctagcggcag cccatctccc tggccggcgg caatctggcg gtcaacctcc
      421 tcggccgcga cgagcgcctg gtccggtgtc accgtgatga aagaatggag cgtgtcattc
      481 aaccgcgtta tccgttccag ggccgcctga atcaccgcaa cggcggtcag ttcgcgccgc
      541 cggaccgact gcgcgatcgt ccgggccgtg gccctcgcaa ggtcgatcat gccggccact
      601 cctcgacaac cacaattacc tccggctcgg cgccggcgcg gtcccgcccg gtaacctggc
      661 ggatctcgcc gataagcggg ttcagtacag agtgaattgc gtccaactcg gccggcgtca
      721 gcggaagcat cacgtcctcg ccggcaatcc ggcgaatcgt ttccacgtcg ataacaggat
      781 cggggaactc gggcattaac aggttcctcg catcgagcgg gggtgacgga tcttccccag
      841 tcgaatgtcg ggagcagcta ctgggtgcag taaaatcaca cgcccggacc agcgagataa
      901 tgtgcccgaa acgaccgtcc ggcacaataa aaatccccca cttccgaaac ctgggacggg
      961 atggaagcgg ggtgcgagag aggctcgtcc tacccgcgac tggggagatt tgggtttccg
     1021 gcaaattgga tgacctttcc aggggttgca gaccgagaag cgcccatcga aactggtaag
     1081 tctggcgcaa acgcctagcc acgatccgct ttcaaatcgg gcgcccaatc atccatggtc
     1141 caccagaagt cgagaggctc gctcaaacgg caacggtatc tttctcttct tccaggagaa
     1201 atggtactcg tctgggccgc cttgttcggt tgcggttcga gcgacggatt gaaccgtcaa
     1261 gcgatctccg ggaaggtcac ccttgacggc gaccccctta cgagcggggc gatccttttt
     1321 gaacccacaa gcaaagattc gggtacagcc gtcggggcga cgatccgtca gggctcgttc
     1381 gcggtatcga gagccgaggg ccctgtcccg ggttcgtatc gagtgcgcgt ctacgccagc
     1441 actagtaagc aggctccgcc cgccaaaggg caaaccgatc gtacgcctcg ccccatggtc
     1501 gaacgcctgc cccctcacta caatacacga tcagagctac gggccgaggt tgtcgctgat
     1561 cgagtcaata ggtatcgatt cgacttggat tcaagcgagc ctggcggtgc ccggtgaccc
     1621 gtcttcacac tcggtgatac cacttcgcgt agcggtccta catgtggagt atcgctttta
     1681 tgaaatccca gcggcgcggt ttcacactga tcgagttgct cgtcgtcatc gcgatcatcg
     1741 ccgtcttgat gtcgctcttg ctgccggccg tgcaatcggc tcgcgaggcc gcacggcgtt
     1801 cgcaatgcgt ctgtaatctc aagcaggtct ccctggcgat acatggttac cacgacaccc
     1861 acagcgtgct accgcccggg aagaaaggct gctgctgggg cacatggctc gtctacactt
     1921 tgccctatct cgaacagaaa cccctttata acgcctggaa ctcattcggc actaatgtcg
     1981 ctggagcgcc ctcgaactat gacctcgatc tacgctactt tggcgtggcg aaccagaccg
     2041 tgacctcgac ttttatcagt acctatctgt gtccgactga tcagaccaac actccgatca
     2101 ccgccacaac caacggcaag acgtacgcct gcacatcgca aaactatgct gtcaacttcg
     2161 ggaatacggt ggtgatccag accgattttc aagacatacg cttcggcggc gctccctttg
     2221 ttgatatcgg ttcacccaat ggcgaccacg atcagacggg acgtgccacc gtcggtttca
     2281 acacgtttgc ggatggactc tccaacacaa tgctggcctc cgaggttgtc gtgggacagg
     2341 ggcaagacct tcgtggattc tcatggtggg gagacgccgc cacatttgag gcgttcctgg
     2401 caccgaacag ctcgttcccc gacgtcatgt tcagtccgtt ctactgcatc aaccagcccc
     2461 ccaacccgcc ctgcatcggc accacgaccg cgctacccga caactacggg gcacgaagcc
     2521 gacacccggg aggcttgaac gtcgcgatgg gggatggctc ggtccgcttc gtcaaaaact
     2581 ccgtcgacat acgcacctgg agagcgatga gtacgacccg cggtggcgag gtcgccagcg
     2641 ccgaccagta ctgacgcatc gacgacggaa agtcaggcgt gtgagccaag agatcgtttg
     2701 ctgtccgctt tcgctcgagt gcgagtcgat gaccgagatt ggcggagatc gacaacattc
     2761 gtccggcgtt tactcggaac gagtgccggc gatcgaccgc ctcgccccgt ctggtcgccc
     2821 gtcgcaacgc gcggtcatgc ggcaaaaatg gcgggacctg ctctttgttc actggccgat
     2881 cgcgcctgag gccttgcggc cgctggttcc tccgcaactc gagctggacc tttttgacgg
     2941 aagagcctac gcgggtttgg tcgcgtttac catgaccgga gttcgcccag tgggcctgcc
     3001 cccggtcccg gggctttcga gctttcacga gaccaacgtg cgaacatacg tgcgattggc
     3061 ggatcgtgac cccggtgtct ggtttttcaa cctggaagca gcgagcaaga tcgccgtgcg
     3121 actggcacgg tggtcgtttc accttccgta ccattacgca cacatgttcc tggaacatga
     3181 gcccaccctt cgggccgacg agccgaactc gatcgtctac gcgggggcgc ggcattggcc
     3241 gggtccgctg ccggcctctt acatcattcg ggccagaccc gacagcccta tccaaccggc
     3301 tcaacccggc tcgcttgagc atttcctggt cgaacggtac atcctcttta ccgtccggaa
     3361 aagtcggctc tatcagggcc gagtccacca cactccctat cctcttcaac cggcaagagt
     3421 tctctctctc gatgaaactc tgttagccgc gaatgggatc gatcgacccg acacccctcc
     3481 gctcgcccat ttcgccagag gagtcgacgt cgaagtcttc ccattaatac gttcctaaat
     3541 caatctcccg gaagcgtcac ccgcgagacc tacccttttc accatgtcac gacgcggatt
     3601 tattacaaat ccgcttcgcc ggcccgctgc ttgaccaact ctgacaagac ttatccgccg
     3661 tcacagttgg ttgggtggcg cggcgtgatt ctaccaactc ccgagcgctt acctaaacgc
     3721 gatcgagggt tgcatgcggc acggctacat aggcctgaaa cgaaaaaacc cgttggaata
     3781 agaatcaagt tatatcatgt catcgagatg caactgcaat tgacgccttg ttgttttttt
     3841 tcaacatcgc ggaaatgtct tgcatttcgg ggaagcacat cccatcctta gcgactcgca
     3901 tgggtactgc tgggaaacct gaagcctact ttcccggatt cgggtggtcc agcggcgtgt
     3961 atcggagccg taaggtgcgg ctcgggccca tgttcctcgt gggagtcttg tccattgcag
     4021 atccctatga accgctcgcg ctggatgaac acagtcgtta ggttatggtt tgggtgtgcg
     4081 gcattctcgg ccgtcgcgat gacggcgcaa gcccaaacgt actactatta tccgacgacg
     4141 gtggcagccg ctcccagcgt ggtcactggc tcgaactttg tcccgaccac ttacgtgggc
     4201 ggggctgtgg gaaacaaggt cacttggtac acgtatccag ctcagtcgaa ccaaactgcg
     4261 tatacggtac aaaacccaca gaccaatgtt gcgccccagt attatcaacc catgtatcaa
     4321 gctcagagtg tgcaagcacc ctctgttaat caggtcgccc cgaccaacaa cacgacgtcg
     4381 aatgccgctc ccgcgacgac gtcgaatgcc gctcccgcgg cggcggtcac ggcgagcgct
     4441 gaagcgggcg atccgtacgg cttcctggga tggctcaacg ccactcgggc gtcctacggc
     4501 ttgccagcag ttggttacga ccccaatctg tcaggctggg cggcgatgaa taacgaccag
     4561 caagccgctc gaggcatggg ccattttgtg atgggtcccg cgcgacgaca gaattcggcc
     4621 atgggtgcat tcgccaacat cgggtcgatg tggatgaatt cgccggccca tcgtgcggcc
     4681 ctactcgatc ccacgattcg cgcaatcgga atcgcggggt tgggtgcgta ttggacgttc
     4741 aacgcgtact gaaaagtgtc gcaaccgatg tcgggttctg aataaacaga aacccactgt
     4801 gatgacatcg ccgttacaga aagagcgcgt cccggatgcc ggccaggatg gcttgctcca
     4861 ggtcgaggtc tcgtccccgc tgaatccacc ccactggagc ggggacggga ccgacgatct
     4921 cacgcgtgcg gttgaccggg aaatcgttgg taaagaccgc acggcccgcc gcttgccggt
     4981 cgggcttgat catgtcttcc aaaaatttgt acacctcgac cttgaccagg aatccgccgg
     5041 cgggcgccgg atcgatgtgg acgatggcaa ccctccggat cgtctgcagg ctcgcctcta
     5101 ttcgatcctc gatcttcact gagtcgaaag cccacggttc cagcagggtc gcccccatct
     5161 gcggctgcgt cttgatcgtt cgtgcgagcc tgttttccga ctcaatgtca aagtacttgt
     5221 cgacgacggc gaccgtcttg ttccacaagg tctcgaagtc ggctgaaggc accggcagcg
     5281 ggttggcagc aacaggccga ggcgcaaaaa tgcgctg
//
LOCUS       DATAQL010000909         6905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4520236, whole genome shotgun sequence.
ACCESSION   DATAQL010000909 DATAQL010000000
VERSION     DATAQL010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4520236"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHS97_15755"
     CDS             <1..235
                     /locus_tag="VHS97_15755"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3449710.1"
                     /translation="ALDLAPIRVNAVVPGLIETPLWDSFGPHREAILARGAKLPVGRI
                     GQPQEVAEAVIFLMSNGFVTGTSLTIDGGGSLV"
     gene            complement(252..1379)
                     /locus_tag="VHS97_15760"
     CDS             complement(252..1379)
                     /locus_tag="VHS97_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449711.1"
                     /translation="MKLPGKQFTVANLMSIVLVTALGLAGLMNSTPIWANSLFTLRRG
                     VGDRETTASVRKGADVRLSSQNLPDIENVTEADIERAFDNGAIGKFVHLWSSADNFIQ
                     AGSRGTPSKCVPPDHPALKDHWAFIHRTGSEPWTLEHIELVDVREYEPEEYLTLEEVK
                     RVFVAYLRGDPDWKQPYTWVQAGQGASKPRAVVITEAEWLSFTQPITMLDLLRGKASD
                     SNLRLFAIACCRRIWPHIADERSRRAVELAALDVDGLVSAEERVAAAQAAADALTDAF
                     FNLDIRSNGHLYHAAWAAALCLHTAEIPLRTPISEPVISGAFDCAVNAAINSASAAAI
                     AKVDITCSKDQMHAQLEVDTNAEYAAQSDLLRDIFGYPFGA"
     gene            1472..2362
                     /locus_tag="VHS97_15765"
     CDS             1472..2362
                     /locus_tag="VHS97_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3449712.1"
                     /translation="MAESPGKMQLGTDRQPSSACRPAPERSPWYRFVDMKDLQQVLRL
                     AELFDHVEDVLVWVKDRDGRYCWVNRAFLINYVTAGYQDQGQLAAELQAIVGKTDYDL
                     SPAFLADQFRLDDEHVLTGKRIVNRIELVGQPDGLTVWNVTNKIPLFDMDGEIIGTAG
                     ITRRLNSPAPETVPGSEFGAVLTYMRDHYRSPITNGELARLAHMSVRAFERKFRSSFH
                     LTPQKYLRKLQMRMASRALVYSSESLAKVALSCGFADQSHFTREFRRHFGRTPRDYRE
                     HYGQGAGGAASGTNSAAGAQ"
     gene            2439..3860
                     /locus_tag="VHS97_15770"
     CDS             2439..3860
                     /locus_tag="VHS97_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEX3449713.1"
                     /translation="MVKRLALMFPIAGFLIASLAWAGDETVPDGFVSLFNGKDLTGWK
                     VPAGDNGHWKVVDGAIDYDASSEAKGDKSLWGEREYEDYVLKVDWRLKEAPFINKGVP
                     YILPDGTHAKDIHGKEMRMALPDADSGVYLRGDGFYQINIWCWPIGSGEMYSLRTDRK
                     TPPELRAAVTPRTQADKPVGQWNHFEITVKGKTVNVILNGVTVIPEATIPTLPDRGRI
                     AFQHHGGKNKDGAWTGPPSLVQFKNIYIKELPASSSAGAVPDKVRALLITGGHDHETA
                     FYSLFEGYKDLGSIPVAASATAFQNDLRDKYDVVIMYDFSRDLDEKGKKNLRDFVESG
                     KGVVVLHHALLNYQKWNWWYQDVVGGSYRLSREGSVPSSTVKNDQDISVAPEGDHPIT
                     AGIAPFHIVDETYGRMWISDKVRPLLSTDNPNSNHLVAWIGPLAGSKVVAIQLGHGNT
                     AFGNPSYRAIVHNAILWAAGRTK"
     gene            3935..4897
                     /locus_tag="VHS97_15775"
     CDS             3935..4897
                     /locus_tag="VHS97_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3449714.1"
                     /translation="MKLGLYSITYLGLWYRGEALPLPEMIKRAKEYGYDGIEIDGKRP
                     HGSPLDWPAKKCRDLRSVADGEGIDIFAVAANNDFSNPVPEVREAQICYVRDMIGMTA
                     DMGAKTLRVFLAWWGVTRHPQLATYDIAEGYWPIVHEKFTKEEIWGWCRESLVECARY
                     AGDAGITLALQNHKPLIDDHHDVLRMVKEVGSPHLKVSLDAPLMPDKSAATMLKAARE
                     VGPLQVLTHFGGEYDRRPDGTIRGYERNDGVVGQDTNEYYRDFVRAMADIGYEGYISY
                     ELCHQLPVVNGQTVDISYANHNAQLAAEFMRELIEAELVPASGR"
     gene            4940..5749
                     /locus_tag="VHS97_15780"
     CDS             4940..5749
                     /locus_tag="VHS97_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX3449715.1"
                     /translation="MRFTCQSFVLIMILGLAGAAAAQDQPNKRKLLFIGQSKGYQHDS
                     VSTAMATLYNLGRSTSLWETYFRTDCTAITKKKLKWGAKNLDAFDAVVFFTDGDLDMD
                     ESQKADLLSFIRDDGKGFIGIHSATITFLSWPEYGKLIGGYFDGHPWGEFQAPLVVED
                     TGFPGMKYLSKSFTLKDEIYQIKDFSRDNVRVLLRLDADKVDLAKKGIHRKDKDFAVM
                     WARNYGKGRVVYNGLGHTQEVWDRPDIQKMWVETVQWSMGIIPGDASPRPE"
     gene            complement(5753..6595)
                     /locus_tag="VHS97_15785"
     CDS             complement(5753..6595)
                     /locus_tag="VHS97_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3449716.1"
                     /translation="MKRIVSCFTNSYGDAGVWAAAERIRDAGIDHIELALRGHNFGGL
                     VIPESVVITEKADDQTAQSFCDHLHRHGVNVSGCNVGGADIRTQEGVELTARRIRFAA
                     RWFNVKICVSGAGQPTDAAERRTIIAHLRRLGDVAGECGLDLALETHKGPTQNAQAML
                     KLMTDVDHPHVRLNFDTGNIAYYNKGVDPCDELDKVKQLVRNVHVKDNRGGHEDWFFP
                     ALGEGGSVDFGRVRAILDGVGFLGPYTIEIEGIGGEPEPGLEGRHERVRRSVAHLRSC
                     GYFD"
     gene            complement(6671..>6905)
                     /locus_tag="VHS97_15790"
     CDS             complement(6671..>6905)
                     /locus_tag="VHS97_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449717.1"
                     /translation="AGSLVANVSDLKHLVRWHTGGRDHSKPPSLLQGWRGEVCGPLLL
                     DVLDGKLALRVVDPDSEYPVALEKLAAKPRPSD"
BASE COUNT         1437 a         2017 c         2093 g         1358 t
ORIGIN      
        1 cgccttggac ctggcgccga tccgggtcaa cgccgtcgtt cccggcttaa tcgaaactcc
       61 gctctgggac tctttcggac cgcatcgcga ggcgatcctc gctcgcggcg ccaagctacc
      121 ggtcgggcgc atcggccagc cgcaagaagt cgctgaggcc gtgatctttc tgatgagcaa
      181 cgggttcgtc acgggaacat ccctcacgat cgacggcggc gggtcgctgg tttgacggtc
      241 gagataacac ttcacgctcc gaacgggtag ccgaagatgt cgcggagcag gtcagactgc
      301 gcggcgtact cagcattcgt gtcaacttcg agttgtgcat gcatttgatc tttagaacat
      361 gtgatatcga ccttcgcaat ggccgccgca gaagcactgt ttatggccgc attcacggca
      421 caatcgaatg cccctgagat cacgggctcg gagatcggcg tacggagcgg aatctcagcc
      481 gtgtgcaaac aaagagcggc ggcccaggct gcgtgataca ggtggccgtt ggagcggata
      541 tccaggttga aaaaagcgtc ggtgagagca tccgcggccg cttgtgctgc ggcaacgcgt
      601 tcctctgcgc tcaccagccc atcgacgtcg agcgccgcca gctctacggc ccttcggctc
      661 cgttcgtcag caatatgagg ccagatgcgg cggcaacacg caattgcgaa caaacgcaaa
      721 ttactgtcgc tggccttgcc ccgcagcaag tccagcattg tgatcggttg agtgaacgac
      781 agccattcgg cctcagtgat cacgaccgca cgcggtttcg aggcgccttg cccggcctgc
      841 acccatgtgt atggctgctt ccaatcgggg tcgccccgga gatatgcgac gaatacccgt
      901 ttgacctctt ccaaggtcag gtattcttcg ggctcgtact cccgcacgtc taccagctca
      961 atgtgttcca gggtccaggg ctctgagccg gttcggtgga tgaaagccca gtgatccttc
     1021 agcgcgggat gatccggcgg cacacatttg gagggggttc ccctgctgcc ggcctggatg
     1081 aagttatcgg ctgagctcca gaggtgaaca aacttgccga ttgctccgtt gtcaaaggcc
     1141 cgctcgatgt cagcctcggt gacgttctcg atgtcgggca agttctgaga agacaatcgc
     1201 acgtctgctc ccttccggac cgaagccgtc gtttctcgat cacccactcc tcgtctgaga
     1261 gtgaagaggg agttggccca tatcggcgtc gagttcatca gcccagccag acctagcgcc
     1321 gtcacgagta cgatgctcat gagatttgcg acggtgaact gcttccccgg caatttcatg
     1381 gcagtttctc cacgccagca gattgtactg ctcctggctg gcaagtgcat cccccacagc
     1441 gaaccctttc tcgacgggag gccctttcgg cgtggccgag agtcctggca aaatgcaact
     1501 gggaactgac cgtcaacctt cgtccgcctg ccggcccgcg cccgagcgat cgccgtggta
     1561 ccggttcgtc gatatgaaag atcttcaaca ggtgctgcgt cttgccgagc tctttgacca
     1621 cgtcgaagac gtcctcgtat gggtcaagga tcgtgacggc cgctactgtt gggtaaaccg
     1681 cgcgtttctc atcaactatg tcacggcagg ctatcaggat cagggtcaat tagccgccga
     1741 actgcaggcg atcgtgggca agacagacta cgacctgtcg ccggccttcc tggccgatca
     1801 gtttcgcctc gacgatgagc atgtgctcac cggcaagcgg atcgtgaacc ggatcgaatt
     1861 ggtgggccag ccggatggtc tcacggtctg gaacgtcacg aacaagatcc cgctcttcga
     1921 catggatgga gagatcattg gcacggcggg tatcacccgg cgactgaact caccggcgcc
     1981 agagacggta cctgggtccg agtttggcgc ggttttgacg tacatgcgcg atcactaccg
     2041 ctcgccaatc acgaatggag agctcgcgcg gctggcgcac atgtcggtgc gggcgttcga
     2101 gcggaaattt cgcagcagct ttcacctaac gccccagaaa tacctgcgca aactgcaaat
     2161 gcgcatggcc agccgagccc tggtttacag cagcgaatcg ctggcgaaag tcgctctttc
     2221 gtgcggattt gccgatcaga gtcactttac acgagagttt cgccgtcact ttggccgcac
     2281 gccccgagac tatcgcgagc attatggcca aggggccggc ggtgccgctt ctggcacaaa
     2341 ttctgccgct ggtgcacaat aaccggcgcg gccgttacgg gtatgctgga gccacagaat
     2401 agatcgaacc cacccttgga cgaattggac gcgcaatcat ggtgaaacga cttgccttga
     2461 tgtttcccat cgcgggattc ttgattgcct cgctggcgtg ggccggagac gagacagtac
     2521 ccgacggctt tgtctcgctt ttcaacggta aagaccttac cgggtggaaa gtgccggctg
     2581 gcgataacgg ccactggaag gtcgtcgatg gagcgatcga ctacgatgcc agcagcgagg
     2641 cgaaggggga caagtcgctc tggggcgagc gcgagtacga agattacgtc ttgaaagtgg
     2701 attggcggct caaggaagca cctttcatca ataagggcgt tccctatatt ctgccggacg
     2761 gaacccacgc caaggacatc cacggcaagg aaatgcggat ggcgctgccg gacgcggatt
     2821 cgggggtgta cctgcgaggt gatggctttt accagatcaa catatggtgc tggcccatcg
     2881 gttcgggcga aatgtacagc ctgcgtaccg accgcaaaac gccccccgag ctgcgcgcgg
     2941 ccgtgacgcc gcgaacgcag gccgacaagc ctgtgggcca gtggaatcat ttcgagatca
     3001 ccgtgaaggg gaaaacggtc aacgtgatat tgaacggggt gaccgtcatc cccgaggcca
     3061 cgattccgac ccttccggac cgcggccgga tcgcatttca gcaccacggc ggcaaaaaca
     3121 aagatggtgc ctggactgga cccccaagcc tcgttcagtt caaaaatatc tatatcaaag
     3181 aattgccggc cagctcgagc gccggcgccg tcccggacaa ggtgcgggcc ttgctaatca
     3241 ccggcggaca cgatcacgag acggcgtttt actcgctgtt cgagggctac aaagatctcg
     3301 gatcgatccc ggtggcggct agcgccacgg cttttcagaa cgatctgcgc gacaaatacg
     3361 acgtcgtgat catgtacgac ttcagccgtg acctggacga gaaagggaag aaaaacctcc
     3421 gcgacttcgt cgagagcggc aaaggggtcg tggtcctcca tcacgcactc ttgaactacc
     3481 agaaatggaa ctggtggtat caagacgtgg tcggcgggag ctatcgcctg tcgcgcgagg
     3541 gaagcgtacc atcttcgacg gtcaaaaacg atcaggatat ctctgtggca cccgaaggcg
     3601 accatccgat caccgcggga atcgcgccgt ttcacattgt tgacgaaacg tacgggcgga
     3661 tgtggatatc agacaaagtt cggccgctac tttccaccga caatcccaac agtaatcact
     3721 tggtagcctg gatcggccct ctggccggct cgaaggtggt ggcgatccag ctgggccatg
     3781 gcaatacggc gtttggcaat ccgtcgtatc gagccatcgt gcacaacgcc atcctgtggg
     3841 cggcgggacg aaccaagtaa cggccctatt gtgcccggcc attttccggg cttgtgttcc
     3901 ggaccgattg gacgctacga gaaagggaat cgccatgaaa ctcggacttt acagcatcac
     3961 ttatctgggg ctttggtatc gcggtgaagc tctgcctttg ccggaaatga tcaagagagc
     4021 caaggaatat ggatatgatg gcatcgagat cgacggcaag cgtccgcatg gcagcccgct
     4081 cgattggccc gcgaagaaat gccgcgatct gcgttcggtt gccgacggcg agggaatcga
     4141 cattttcgca gtggccgcca acaacgactt cagcaacccg gtccccgagg tgcgcgaagc
     4201 ccagatttgc tacgtccgcg atatgatcgg catgactgcc gacatgggtg ctaaaacgct
     4261 gcgggtgttc ctggcgtggt ggggtgtgac gcggcatccg caactggcca cgtacgacat
     4321 tgccgaaggg tactggccga tcgtccacga aaagttcacc aaagaggaaa tctggggatg
     4381 gtgccgcgag tcgctggtcg agtgcgcccg ctacgccggc gacgcgggca tcacactggc
     4441 gctccagaat cacaaaccac taattgacga ccatcacgac gtactgcgca tggtcaaaga
     4501 ggtcggctct ccgcacctga aggtcagtct ggatgctccg ctcatgcccg acaagagcgc
     4561 ggccacaatg ctcaaagcgg cgcgtgaggt cggcccgctc caggtgctca cccatttcgg
     4621 cggtgaatac gaccggcggc cggacgggac gattcgtggt tatgagcgga acgacggcgt
     4681 ggtcggccaa gacaccaacg agtattaccg cgatttcgtg cgggcgatgg ccgacatcgg
     4741 ctacgaaggc tatataagct acgagctttg ccatcagttg cccgtcgtca acggccagac
     4801 cgtcgacatc agctacgcca accataacgc ccagctcgcg gctgagttca tgcgcgaatt
     4861 gatcgaggcc gagctagttc cggcgagcgg aagataatcg actggggtca atttcacccg
     4921 ctgacttcgg gaggcgacga tgagattcac gtgccagtcg tttgtgctga tcatgatatt
     4981 gggactggcc ggcgctgccg ccgcacagga ccagcccaat aagcggaagc tgctcttcat
     5041 cggccagagc aaaggttatc agcacgattc ggtgtcgacg gcgatggcca cgctctacaa
     5101 cctgggtcgg agtacgtcgc tctgggaaac ctatttccgg accgactgca cggccatcac
     5161 caaaaagaag ctgaagtggg gcgccaagaa cctcgatgcc tttgatgccg tggtgttttt
     5221 cacggacggc gatctggata tggatgaatc gcaaaaggca gacctgctgt cattcatccg
     5281 cgatgacggc aagggtttca tcggcatcca cagcgccacc atcacgtttc tctcgtggcc
     5341 cgaatacggc aagctgattg gcggatactt cgacggccac ccctggggcg agtttcaggc
     5401 acctctggtg gtggaagata ccggttttcc cggcatgaag tacctgtcga agagcttcac
     5461 gctcaaggac gagatctatc agatcaagga tttttcacgc gacaacgttc gtgtgctctt
     5521 gcgccttgat gccgacaagg tcgatctggc caagaaaggg atccaccgca aggacaaaga
     5581 cttcgcggtc atgtgggccc gcaattacgg caaggggcgc gtcgtctaca acggcctggg
     5641 ccacacccag gaagtgtggg atcggcccga catccagaag atgtgggtgg aaaccgtgca
     5701 atggtcgatg ggtatcatcc ccggcgacgc cagccctcga cccgagtgaa catcagtcaa
     5761 agtaaccgca cgacctcaga tgggcgacgc tacgacggac gcgctcgtgt cggccttcca
     5821 ggcccggctc gggctcgccg ccgattccct cgatttcgat cgtgtaagga ccgagaaagc
     5881 cgacgccgtc caggatcgcg cgaacccggc cgaaatcgac ggatccacct tctcccagcg
     5941 cagggaaaaa ccagtcttca tggccgccgc ggttgtcctt gacatggacg ttgcgcacaa
     6001 gttgcttgac cttgtcgagc tcgtcgcagg gatcaacacc cttgttgtag taggcgatgt
     6061 tgccggtatc gaagttgaga cggacatgag ggtggtcgac gtcggtcatc aatttcagca
     6121 tggcctgggc gttttgcgtc ggtcctttat gcgtctcgag cgcaaggtcg aggccgcact
     6181 cgcctgcgac gtcgccgagc ctgcggaggt gcgcgatgat cgtgcgtcgt tccgcggcat
     6241 ccgtcggttg accggcgcca gaaacgcaga tctttacgtt gaaccacctg gccgcgaatc
     6301 ggatccggcg cgcggtcagt tcgacgccct cctgagtgcg gatgtcggcg cccccaacgt
     6361 tgcagccgct cacattgacc ccgtggcggt gcagatgatc gcagaatgac tgggccgttt
     6421 ggtcgtcagc tttttcggta atcacgacgg attcggggat caccagacca ccgaaattgt
     6481 ggccgcggag cgccagctcg atgtgatcaa tgcctgcgtc ccggattcgc tcggccgcgg
     6541 cccacacacc ggcgtcaccg taactgttgg tgaaacaact gacgattcgc ttcatggttg
     6601 taggccgtgc ctccggcggt cgggattcta tattgatgag gttttgagca catgaattac
     6661 caacaaccct tcagtctgat gggcgtggct tggccgcgag tttttcgagc gcaacggggt
     6721 attcgctgtc ggggtcgacg acgcgcaggg ccaatttgcc gtcgagcaca tcgagcaaga
     6781 gcggtccgca gacttcgccg cgccagccct gaaggaggga agggggcttt gaatggtcgc
     6841 ggccgcccgt atgccagcgg accaggtgct tcaaatcgct gacattggcg acgagcgagc
     6901 cggcc
//
LOCUS       DATAQL010000910         9371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2385622, whole genome shotgun sequence.
ACCESSION   DATAQL010000910 DATAQL010000000
VERSION     DATAQL010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385622"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..442)
                     /locus_tag="VHS97_15795"
     CDS             complement(68..442)
                     /locus_tag="VHS97_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3449718.1"
                     /translation="MSSTSDFQHLRLSRVKDIVIVEIISKDLQGPAAARELGAELSLV
                     SAQDWAKRIVLNFHSINYLSSTGFAVLLKLVRNVTAAGGQVAVSDMEPGLRLGAEIVG
                     LDKVVPIYDSEAAAIASFDKNS"
     gene            866..2035
                     /locus_tag="VHS97_15800"
     CDS             866..2035
                     /locus_tag="VHS97_15800"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX3449719.1"
                     /translation="MERSDSLTHVVILGSTGSIGHSAMSVIEHDGGTRLKAWGLSAQS
                     RWKVMAQQAIAHRPRFVAVADAELAPLLKDELRGTGVEVLVGLDGVIRMVQDQETDRV
                     LSAIVGAVGLRGTWAALEAGKTVALANKETLVVAGPLIMELANARGAQILPVDSEHSA
                     IFQSLKAGARTDVRRVILTSSGGPFRGRSKKQLTEVTPEQALNHPTWRMGPKITIDSA
                     TLMNKALEVIEARWLFQLEPDQVEVVIHPESVVHSMVEFVDGSVIAQLSPPDMRLPIQ
                     YALTYPDRTPGAGQRLDLEKSFSLHFEPPDRETFPSLELGFEVMRRGGTAGAALNAAN
                     EAAVARFLNGEIGFLDIARACKAALDHHSFDPRPTLDSLWDVDAWARREVDRWKP"
     gene            2023..4056
                     /locus_tag="VHS97_15805"
     CDS             2023..4056
                     /locus_tag="VHS97_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3449720.1"
                     /translation="METLNFLLTIAKVAVGLGFVIFIHELGHFLLAKWNGVKVEKFSI
                     GFGPTIFGWRRGETEYVLAALPLGGFVKMLGEGPEDQENKTTDPRAYPNKSVSARMAI
                     ISAGVIMNVFLAVACFVYYYGQERLEQPAVLGGVTAGSPAYEAGLRPGDEIVAIDERR
                     NLDYLGLIEKVLFSSQGQVLHFEVRRPGHDGLIGVDIQPRREATGDRPTIGVLAGRSL
                     EIGGFEPAAGMENPPVYHGLEAKERESKVDVLVAAGPTGKESVPLADKFEYDRELARN
                     ADQPITHMIERRPISPSRVHSAAVDRSELTLPANHFVDFGFHLAFEPIRAIRKDSPAD
                     KAGFRKGDLIVSVDGKDDVDPMQLPTDCLAKAGKPMTFEVQRDAGAGQRKTQSLTVTP
                     DDTPPRTEFATPDIHEPVDVAGLGLCYPVSTRIVGVRPNSPASKAGLKPGDVLSALAI
                     PPSKPQPTISGWKAWLPSFLGGAKVEQPKTFEFSEESPDWLAAFLYLQNLPLQEVELT
                     VNRASKPIKITPEIDRSWHNPYRGLLFYPLVRMAPPQTLSAALRSGYEHTIQNILMVY
                     GTFRSLAERRVSPKNLGGPILIAQVAYSAAGSGFAELIKFLGMLSINLAVLNFLPIPP
                     LDGGQMLFLIAEKIRGRPLPDSALIAGTYVGLLLVLCLMVFVTYQDVFRLFTG"
     gene            4149..4859
                     /locus_tag="VHS97_15810"
     CDS             4149..4859
                     /locus_tag="VHS97_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449721.1"
                     /translation="MGMLRDRLVLADGPARLRGNGETVTDVVGRLEAGELPVKLVRPN
                     GLLAADLIAALAQAALGDDHSMGPALVQASPASRTLLPSLSEPAWVAVFPSAPHRTRL
                     SLSAGLLQIHDFWDASHDAAQRADDVGERDFSAYWHGIAHRREPDAGNAAYWFRRVGK
                     HPVFKPLAEAGRTLLDQEGDAQLANRLIPGGDWNPMVMIELCTQARPGTREESVARKL
                     QRLEMWLLVEATCAAITG"
     gene            4956..5549
                     /locus_tag="VHS97_15815"
     CDS             4956..5549
                     /locus_tag="VHS97_15815"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3449722.1"
                     /translation="MAIGLQELETQPPALMDSDAAQFYEVVDGKIVENPPMGARQSIL
                     ASYLLLRIGQIASSNRLGWAVNETLFLIDPARKLKRRPDVAFVSAKRWSLKQEVPDTE
                     TWDVVPDLTVEVVSESNSANAVARKIEEYFQAGVQKVWVIYPSTSKIYVYDSPTRVRI
                     LQLGDELEGEAIIPGFHVPLSTLFKLNEANEASNQTE"
     gene            5567..7543
                     /gene="acs"
                     /locus_tag="VHS97_15820"
     CDS             5567..7543
                     /gene="acs"
                     /locus_tag="VHS97_15820"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX3449723.1"
                     /translation="MISDHGGGSITSVLQETRVFPPPGEFASNAHISSLAQYETLWNR
                     AKDDPESFWAEHARTLAWDKTWDKVLDWQPPFAKWFVGGQLNAAYNCVDRHCVGATKN
                     KAAIIWEGEPGDRRVLRYQDLQREVSKFANVLKGLGVKKGDVVAVYMPLVPELVIALL
                     ACARIGAPHTVIFGGFSAESLSGRIQDCKAQVLLTADGGYRRGKLVPLKENADAAAAL
                     CPTLKHVVVFRRTGTQVNWSPGRDHWWHELEANASSHCPPEPMDSEHPLYILYTSGST
                     GKPKGILHTTGGYLVGTTLTSKWVFDLKDDDTFWCTADVGWVTGHSYLVYGPLSNGAT
                     CVMYEGAPNWPDEGRFWKIIEDYRVTILYTAPTAIRAFMKWGEQFPRRHDLSSLRLLG
                     SVGEPINPEAWMWYHKVIGGERCPIVDTWWQTETGAIMISPLPGATPTVPGSATRPLP
                     GIVPEIVTKDGQPCPTNHGGFLVIKQPWPSMLRTLYGDDERYKTQYWSDIPGCYFTGD
                     GARRDDTGNYWIMGRVDDVLNVAGHRLSTMEVESALVSHPFVAEAAVVGKPDDMKGQA
                     IAAFVTLESGHQPSEQLRQELRAHVTKEIGALARPDEIRFTDALPKTRSGKIMRRLLR
                     DIAAGVDSTGDTTTLEDLAVLANLRRSDQPMDEE"
     gene            7565..7786
                     /locus_tag="VHS97_15825"
     CDS             7565..7786
                     /locus_tag="VHS97_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX3449724.1"
                     /translation="MGKRYTVSDGKLVLNLEEAEEGGYVVTSPLNPELITQAESIEEA
                     FANARDAAKVLRQSRMKLMRRLSASITCG"
     gene            complement(7819..8139)
                     /gene="rpmB"
                     /locus_tag="VHS97_15830"
     CDS             complement(7819..8139)
                     /gene="rpmB"
                     /locus_tag="VHS97_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX3449725.1"
                     /translation="MGRECPVSGKKTSFGNHKTDRGKAKYLGGVGKKTTGVSRRTFKP
                     NLQRIHIWLPNGTTRYIRVATSVIRSGMMTLEVDGKVQTFPLIKAAKGSAAAREKLKN
                     LYPI"
     gene            complement(8276..8491)
                     /gene="rpmF"
                     /locus_tag="VHS97_15835"
     CDS             complement(8276..8491)
                     /gene="rpmF"
                     /locus_tag="VHS97_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX3449726.1"
                     /translation="MAVPKRRKSPSRQGMRRSHDFLVAPALNICPQCKLAVPPHKVCD
                     LVEECGNIQRSKTHNPLAKKEKEKTKK"
     gene            complement(8655..>9371)
                     /locus_tag="VHS97_15840"
     CDS             complement(8655..>9371)
                     /locus_tag="VHS97_15840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449727.1"
                     /translation="WPWFRNLPEDTPRVNREELRLIESGRSERATGGHSAIPWARMLR
                     TKSVWALCLMYGFLGFSGNFYITLLPTYLRNHRHLSSDITGRLTSLPFALGVVACFVG
                     GSISDVVIRRWGKRWGRRIVGAAGMTVAGLAILAVPWVDNVVALGFLLTLAFSGNDLA
                     MAPAWAAAADIGERFTGTLAGAMNMISSLLAAIQAIAISRFLDSHDLVTPFILLAISY
                     ALGTLAWIGVDMRQTLADPH"
BASE COUNT         1905 a         2811 c         2819 g         1836 t
ORIGIN      
        1 aaccatatac caagaccgca gaggcaatct tcgacccgaa tcgacgcaat cgcatgagtt
       61 actccggtca actgtttttg tcgaaggagg caatggcagc cgcctcgctg tcgtagatgg
      121 ggacgacctt gtcgagaccg acaatctcgg ctccgaggcg gagaccgggc tccatgtcag
      181 aaactgcaac ctggccccct gcggcggtga cgttacggac cagcttcaaa agaacggcga
      241 agccggtact gctcaggtag ttgatgctgt gaaagttcag cacaattcgc tttgcccagt
      301 cctgagccga caccagcgac aactcagcac ctagctcgcg ggcggccgcg ggaccctgca
      361 agtccttgga gatgatctcg acaataacga tgtccttgac ccgcgaaaga cgaagatgct
      421 gaaagtctga cgtggaggac attcttgatc cctcgatcgg ttacagtgtg ttccgctcgg
      481 catcgatcga acgcgcgttc ttaatactgt agccatcact cttgacgaat cgccagccaa
      541 agcagccggc gatggcggta agcatcccgg accatcaatg gccgatggtg aatgccggcg
      601 cccctattgt gacacgggcg ctgtcataat tgctttagtc aaccttattc tgccatattc
      661 gccgggagca acggcgcgga cgcgaagctt cgagccagga cggccacgac ttgtaccgca
      721 tcgggggagt ctctatagtt caggtgggtt gtcggagagc aaagactggc cgcgtgcgtg
      781 cgtgaatcaa gagatattgc cggccgcggg ggctgattgt gccgtgggta gcggtaacgt
      841 ccgtcccgcg gaactaggaa cgcggatgga gcgaagcgat tcactcactc acgtcgtaat
      901 tctgggctcg acgggctcga tcggccacag cgcgatgagc gtgatcgagc atgacggcgg
      961 gacccggttg aaggcatggg gcttgagcgc ccaatcgcgg tggaaggtaa tggcccagca
     1021 ggccatcgcg catcggcccc gattcgtcgc cgtcgcggac gccgaactgg ctcccctcct
     1081 caaggatgag ctgcgcggaa cgggtgtaga agtgctggtg ggcctggatg gcgtgatccg
     1141 gatggtccaa gaccaggaga ccgaccgcgt tttgagtgcg atcgtggggg cagtcgggct
     1201 tagagggacc tgggcggctc tcgaagccgg taaaacggtc gctttggcca acaaagagac
     1261 gctggtcgtg gccggaccgc tgatcatgga actggccaac gcacgcggag cgcaaatctt
     1321 accggtcgac agcgagcatt ccgccatttt ccagtcgctc aaggcaggtg cgcgcaccga
     1381 cgttaggcgc gtcattctaa cgtcatccgg cggcccattt cggggccgct ctaaaaagca
     1441 actgacggaa gtgacgcccg aacaagccct gaatcatccc acctggcgga tgggccccaa
     1501 gatcaccatc gactcggcca ccctcatgaa caaggcgctc gaggtcatcg aagcacgctg
     1561 gctgttccag ctcgagcccg atcaagtgga ggttgtgatc catccggaga gcgtggtaca
     1621 ttcgatggtc gagtttgtgg atggaagtgt aatcgcacag ctttctccgc ccgacatgcg
     1681 tttacccatc cagtacgccc tgacttaccc ggaccggacg cccggagccg gtcagaggct
     1741 cgatctggaa aagtcgtttt ctctgcactt cgagcctcca gaccgggaga cgtttccgag
     1801 cttggaatta gggtttgaag tgatgcggcg ggggggaaca gccggcgcag cgctcaatgc
     1861 cgccaatgaa gccgcggtag cgcggttcct caacggcgaa atcggctttc tcgatatcgc
     1921 gcgggcgtgc aaggctgcgc tcgatcatca ttcgtttgat cctcgcccca cgctcgactc
     1981 attgtgggat gtcgatgctt gggcgcgtcg ggaggttgac cgttggaaac cctgaatttt
     2041 ctcctcacca tcgccaaggt tgccgtgggg ctcggcttcg tcatcttcat ccatgagctg
     2101 ggtcatttcc tcttggccaa gtggaatgga gtgaaggtcg agaagttttc catcggcttt
     2161 gggccgacca ttttcggctg gcggcggggc gagaccgagt atgtactggc ggcgcttccc
     2221 ctgggaggat tcgtcaaaat gctcggcgag ggacccgagg accaagagaa caagacgacc
     2281 gatccccggg cgtaccccaa caagtcggtc agcgcgcgaa tggcgatcat ctcggccggt
     2341 gtgatcatga atgtctttct ggcggtcgct tgcttcgtct actactacgg tcaggaacgc
     2401 cttgaacaac cggcagttct gggcggcgtc acggccgggt ccccagccta tgaggcaggc
     2461 ctgcgcccgg gcgacgaaat tgtggccatc gacgagcgcc gcaacctcga ttacctcggc
     2521 ctgatcgaga aggtgttgtt tagctcgcag ggacaggtgc tccacttcga ggtgagacga
     2581 ccaggtcacg acggcctgat cggcgtggac atccagcccc ggcgcgaagc caccggcgat
     2641 cgccccacca tcggggtcct tgcgggccga agcctcgaga tcggcggttt cgagccagcg
     2701 gcgggaatgg aaaacccacc cgtctatcac gggctcgagg ccaaggagcg cgagtcgaag
     2761 gtcgacgttc tggtcgcggc cgggccaacc ggcaaagagt cggtgccgct tgccgacaag
     2821 tttgagtacg atcgcgagct cgcacgcaac gccgatcagc cgatcacgca catgatcgag
     2881 cgccggccga tctcgccatc gcgagtgcat agcgcggcgg tcgatcgctc cgagctcacg
     2941 ctccccgcca atcactttgt cgatttcggg ttccacttgg cgttcgagcc gatccgtgcg
     3001 atccgcaaag actcgccggc ggataaggcc ggattccgta agggcgatct gatcgtctcg
     3061 gtcgacggaa aagacgacgt cgaccccatg cagctcccca ccgactgctt ggccaaggcg
     3121 ggtaagccga tgacctttga agtgcagcgc gacgccggcg ccggccaacg gaaaacgcaa
     3181 tcgttgacag tcactcccga cgacacacct ccccggaccg aattcgccac acccgatatt
     3241 cacgagccag ttgatgtcgc ggggctaggt ctgtgttatc cggtgagcac tcgcatcgtc
     3301 ggcgtgcggc ccaattcgcc ggcgagcaag gcaggcctga agcccggcga cgtgttaagc
     3361 gcgctggcca tacccccttc caaaccgcag cccacgataa gcggctggaa ggcatggctt
     3421 ccgagtttct tgggcggggc caaggtcgaa caacccaaga cgtttgagtt cagcgaggaa
     3481 tcgcccgact ggctcgcggc gttcctgtac ctgcagaacc tgcccttgca agaggttgag
     3541 ctgacggtca atcgcgcgtc caagccgatc aagatcacac ccgagatcga tcgcagctgg
     3601 cacaacccgt acaggggcct tctgttctac ccgctggtac gcatggctcc tcctcagacc
     3661 ttgagcgccg cgctgcggag cggctacgag catacgatcc agaacatcct gatggtctac
     3721 ggcacgttcc gcagcctggc cgaacggcga gtcagtccga aaaatctggg cggtccgatc
     3781 ttgattgccc aggtagccta tagcgcggcc ggctcggggt ttgccgagct gatcaagttc
     3841 ctgggcatgc tcagtatcaa tctggcggta cttaatttcc tgcccattcc ccccttggac
     3901 ggcgggcaga tgctcttctt gattgccgaa aagattcgcg gccggccgct gcccgattcg
     3961 gccttgatcg ctggaaccta cgtcggactg ctgttggtgc tctgtttgat ggttttcgtc
     4021 acctatcagg acgtctttcg cctgttcacg ggttgatgtt acggaaaacg cctcaagatt
     4081 tctgcctcga ccggtgagtg gaggggtttc tctcgacggg agaggccgtc gtttcgagag
     4141 gtcgttcgat gggcatgctg cgagatcgat tggtgttggc cgatggcccg gctcgcctgc
     4201 gcggtaatgg cgagaccgtc actgacgtgg tcggccgtct cgaggctgga gaattacccg
     4261 tcaagctggt ccgacccaat gggctcctcg ccgccgactt gatcgccgcc ctggcccaag
     4321 ccgcgctcgg cgatgatcac tcgatggggc cggcgctcgt gcaggccagt ccggcctctc
     4381 gaactctgtt gccgtcgctt tctgaaccgg cgtgggtcgc cgtctttccc tccgcgccgc
     4441 accggacaag gctgagtcta tcggccggtc tgctccagat ccacgatttc tgggacgcca
     4501 gccacgatgc cgcacagcgg gctgacgacg tgggagagcg cgatttctcg gcgtactggc
     4561 acggtatcgc ccatcgccgc gagcctgacg caggcaacgc ggcgtactgg tttcgtaggg
     4621 tcggcaagca ccccgtcttc aaaccgcttg ccgaagcagg tcggactctg ctcgaccagg
     4681 aaggcgacgc gcagcttgcc aatcgattga tccccggggg cgactggaac cccatggtca
     4741 tgattgagct ctgtacccag gcgcggcctg gcactcgaga ggaatcggtg gcgcgcaaac
     4801 ttcagcgcct cgagatgtgg ctcttggtcg aggcgacgtg cgcggcgatc accgggtagg
     4861 ccccatagtg attgagtgcc cgcgatgata gaatcgccgc ggaagcaagc gagctaagtc
     4921 gacttttcgg cagtagagct gggagaagat tcgccatggc catcggtcta caagaactcg
     4981 agacgcagcc tcccgcactg atggattccg acgcggccca gttttacgaa gtggttgatg
     5041 ggaagatcgt ggagaatcca ccgatgggag cacggcagtc gattcttgct tcctatcttc
     5101 tgcttcgcat tggccaaatc gccagctcga atcgactggg atgggcagtt aacgaaactc
     5161 tgttcttgat tgatccagcc cgcaaattga agcgacgccc cgacgtcgct ttcgtttcgg
     5221 ccaagcgctg gtcattaaaa caggaagtcc cagatacgga gacctgggac gtagtcccgg
     5281 acttgactgt cgaggtcgtc agtgaatcca actcagccaa cgcggttgcc aggaagatcg
     5341 aggagtattt tcaggcgggc gtacaaaagg tctgggtcat ctatcctagt actagcaaga
     5401 tctacgtcta cgactcgccg acgcgggtgc gcattcttca gctcggtgac gagcttgagg
     5461 gcgaagcaat tatccccggg ttccacgtcc cgttgtcgac actatttaag ctgaatgaag
     5521 ccaacgaagc ctcgaaccag acggaatagt cgagagtatc gataatatga tctctgatca
     5581 cggcggcgga tcgatcacca gcgtgctgca agagacgcgg gtcttccctc ctcccggcga
     5641 atttgcgagc aacgctcata taagcagcct ggcacaatac gagacgctct ggaaccgggc
     5701 caaagacgac cccgaaagtt tctgggcaga gcacgcacgg accttggcct gggacaaaac
     5761 gtgggacaaa gtgctcgact ggcagccgcc gtttgcgaag tggttcgtcg gaggacagct
     5821 caatgccgcg tataactgcg tcgaccgcca ctgcgtgggc gcgacgaaga acaaggcagc
     5881 gatcatctgg gaaggagagc ccggcgatcg ccgcgtcttg cgctatcaag acctccagcg
     5941 cgaggtctcg aagttcgcta acgtgctcaa aggccttgga gtaaaaaagg gcgacgtggt
     6001 cgcggtctac atgccgctcg tgcccgagct ggtcatcgct cttttggcct gtgcgcggat
     6061 cggcgcgccg cataccgtga ttttcggtgg tttcagcgcc gagtcgcttt ccggacggat
     6121 tcaagactgc aaagcccaag tcctgctcac cgccgatggc ggttatcgcc gcggcaagct
     6181 ggttccgctc aaggaaaacg ccgatgccgc tgcggccctg tgccccaccc tcaagcacgt
     6241 ggtcgtcttt cggcggaccg gtacacaggt caactggtcg ccgggccgcg atcactggtg
     6301 gcatgagctg gaagccaacg cgtcgtctca ctgtcccccc gagccgatgg acagtgaaca
     6361 tcctctgtac atcctctaca cctccggatc caccggcaaa cccaagggaa ttctccacac
     6421 cacagggggc tacctggtag gaacgaccct cacttccaaa tgggtcttcg acctcaagga
     6481 cgacgacacc ttctggtgca cagctgacgt cggctgggtg acgggccact cttatctcgt
     6541 ctatggcccc ctatccaatg gcgcgacctg tgtcatgtac gagggagcac ccaactggcc
     6601 cgacgagggc cgtttctgga aaatcatcga agactaccga gtcaccatcc tttacaccgc
     6661 accgaccgca atccgtgcgt tcatgaagtg gggcgagcag ttcccgcgcc gccatgatct
     6721 atcgagcctt cgcctgttgg gcagcgtggg tgagccgatc aatccagagg cctggatgtg
     6781 gtatcacaag gtcatcggtg gcgaacgatg tccgatcgtc gacacctggt ggcagacgga
     6841 aaccggggcc atcatgatct cgccgttgcc gggagcgact cccacggtgc ctggctcggc
     6901 gaccagaccc cttcccggga ttgttcccga gattgtcacc aaggacgggc agccctgccc
     6961 gaccaatcac ggcggcttcc tcgtgatcaa gcagccttgg ccgtcgatgt tgcgaacgct
     7021 ctacggcgac gacgagcgct acaagactca gtactggagc gacatccctg gctgctactt
     7081 taccggcgac ggagcgaggc gcgacgacac cggtaactac tggatcatgg gccgggtcga
     7141 cgacgtcttg aacgtcgctg gccaccggct ctcgacgatg gaagtcgaaa gcgctctggt
     7201 cagtcatccc ttcgtggccg aagccgccgt ggtgggaaag ccggacgaca tgaaagggca
     7261 agcgatcgcg gcctttgtta cccttgaatc cggccatcag cctagcgagc agttgcgcca
     7321 ggagctgcga gcccacgtca ccaaagaaat cggtgcgctc gctcgtccgg acgagatccg
     7381 cttcaccgac gccctaccca agacccgcag cggcaagatc atgcgccgct tgctccgcga
     7441 tatcgcagcg ggagtcgata gcaccggcga tacgaccact ctggaagacc tggctgtgct
     7501 ggcgaacttg cgccggtcgg atcaaccgat ggatgaagaa tagcaaccag atcagaggct
     7561 gttcatgggt aagcgctaca cggtgagcga cgggaagctg gttctaaacc tcgaagaggc
     7621 tgaggagggc gggtacgtcg tgacttcgcc tctcaacccg gagttgatta cgcaagcgga
     7681 gtccatcgaa gaggcgttcg ccaacgcgcg agatgccgcc aaggttttga ggcaatcgcg
     7741 gatgaagctg atgcgccgac tatccgcatc cataacatgc ggatgagttt gcccgatgaa
     7801 gcgccggcaa ctgcgtctct aaatcggata aagattcttg agcttctcgc gcgccgcggc
     7861 gcttcccttt gccgccttga tgagcggaaa ggtctgaacc ttgccgtcga cctcaagggt
     7921 catcatgccg ctgcgaatca ccgaggtagc gacgcggatg tagcgcgtcg tcccgttcgg
     7981 cagccagata tgaatgcgct gaagattggg cttgaacgtg cggcgactaa cgccggtcgt
     8041 cttcttgccg acaccgccta agtatttcgc cttgccgcgg tcggtcttgt gattgccgaa
     8101 cgaggtcttc tttccgctga caggacactc acggcccatc ggtcccactc ctcgattctc
     8161 gagcccagaa atcgcgattc caggttcaag gattccagtt gctaagatat tctcaaggac
     8221 tcagattctc ggctcaagat aggcaaatcg ttgccagtag atcgatgacg cccggctact
     8281 tcttggtttt ttccttttcc ttcttggcga gcgggttgtg cgtcttactc cgctgaatgt
     8341 tgccgcattc ctccaccagg tcacagacct tgtgaggcgg caccgccagc ttgcactgcg
     8401 gacaaatgtt caatgcggga gcgaccaaaa agtcgtgact ccttcgcatc ccttgtcttg
     8461 atggcgactt acgtcttttt ggtacggcca cgaacccaca ctccgaatcc gatcgagttg
     8521 catctttttt tcaagcaaag agttccatta tgcggatcgc ctgaggcatt tcaagtgcga
     8581 gttgggccca tggcgctctg aaccggctca cattcacgtg tcctgagcaa caaccgtcaa
     8641 tttggcagat gttttcaatg cggatccgca agcgtttgcc tcatgtcgac gccgatccag
     8701 gcgagggtgc cgagagcgta gctgatcgcc aacaagatga acggtgtgac gagatcgtgc
     8761 gagtcgagaa atcgactgat cgcgatcgcc tgaatggctg ccaacagact ggaaatcata
     8821 ttcatcgccc cggcgagcgt gccggtgaag cgttcaccga tgtcggcggc cgccgcccag
     8881 gcaggggcca tcgccaggtc gttcccacta aatgcgagcg tcaagagaaa gcccagcgca
     8941 accacgttat cgacccacgg tacggccaag atcgccagcc cagccacggt cataccggct
     9001 gcgccgacga tgcgacggcc ccatcgcttt ccccaccgcc ggatcacgac gtcggagatc
     9061 gaacctccca caaaacatgc cacgacaccc aaggcgaacg gtaacgatgt gagccgaccc
     9121 gtgatgtcag aactcagatg tcgatgattt ctcaaatacg ttggcaagag ggtgatgtag
     9181 aagttgccgc tgaaacccag gaagccgtac attaggcata gggcccacac gctctttgtt
     9241 ctcagcatcc gagcccaggg aatcgcgctg tgtccgccgg tcgcgcgttc ggaccggcca
     9301 gactcgatca atcgcagttc ctcgcgattg actcgtggag tatcctccgg caggttacga
     9361 aaccagggcc a
//
LOCUS       DATAQL010000911         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2231072, whole genome shotgun sequence.
ACCESSION   DATAQL010000911 DATAQL010000000
VERSION     DATAQL010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231072"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VHS97_15845"
     CDS             complement(<1..981)
                     /locus_tag="VHS97_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX3449728.1"
                     /translation="MPDESRESLEAVRVEKLERIANLGVDPWGQRFDGHQAISVARAL
                     ATDLARDAGHEQPGPTVRVAGRIMLRRGQGKVVFLDLRDWTDHIQIFVGKKQVGELGW
                     SLIEELDLGDLIGVDGHLGYTKTGELTVFAERLTFLAKSVLPPPEKWHGLTDQEQRYR
                     QRDVDLFSNPESLGVFLGRSKLIASFRKTMAEKGYVEVETPTMQAIAGGAAARPFVTH
                     HNALDIDLFLRIAPELYLKRLLVGGMERVFEIGRVYRNEGLSPRHNPEFTMFEAYQAY
                     GDYRSMMDLVECLICGAIEALGGGFARPWGEVTVDFTPPWPRRTYAELLAQ"
     gene            complement(1393..>2552)
                     /locus_tag="VHS97_15850"
     CDS             complement(1393..>2552)
                     /locus_tag="VHS97_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX3449729.1"
                     /translation="AGAGTVPHAHDPSKRIAPSMLTTDLALKFDPTYEKISRRFFENP
                     AELADAFARAWFKLTHRDMGPRARYLGPEVPAEKLIWQDPIPEVNHPLIDAQDIASLK
                     SKILASGLTVSQLVSTAWASASTYRGSDKRGGANGARVRLTPQKDWEVNTPGQLATVL
                     QKLEGIQSAFNGAQKGGKKVSLADLIVLAGCAAVEQAAKKAGHDVTVPFTPGRMDASL
                     EQTDVESFAVLEPIADGFRNYLKGRYSVPAEALLIDKAQLLTLTAPEMTVLVGGMRVL
                     NTNAAKVRHGVFTKRPETLSNDFFVNLLDMNTEWKAVSKEADVFEGRDRKTGELKWTG
                     TRIDLIFGSNSILRALAEVYGSSDAEKKFVEDFVAAWDKVMNLDRFDLARS"
BASE COUNT          483 a          775 c          770 g          524 t
ORIGIN      
        1 ttgagccagc aactcggcat aggtccggcg cggccagggg ggcgtgaaat cgaccgtcac
       61 ttcgccccac ggccgtgcga acccgccacc gagcgcctcg atcgcgccgc agatcaagca
      121 ctcgaccagg tccatcatgg aacggtagtc gccataagcc tgataggcct cgaacatcgt
      181 aaattcgggg ttgtggcgcg gacttagccc ctcgttgcga tagacccggc cgatctcgaa
      241 aacgcgttcc atgcccccga ccagcagcct cttcagatag agctccggcg cgatccgcag
      301 aaacaggtcg atgtcgagcg cgttatgatg tgtaacaaaa gggcgagccg ccgcccctcc
      361 cgcgatcgct tgcatcgtgg gagtctcgac ctcgacatac cccttctcgg ccatggtttt
      421 gcggaacgag gcgatcagct tcgagcgacc gagaaaaacg cccagcgact cgggattgct
      481 gaaaaggtcg acatcacgct ggcggtaacg ctgttcctgg tcggtcagac cgtgccattt
      541 ttcgggaggt ggcaagacgc tcttggccag aaaggtaagc cgttcggcga atacggtcag
      601 ctcgcccgtc ttggtgtagc caaggtgacc gtcgacaccg atcagatcac ccaggtcgag
      661 ctcttcgatc aagctccaac ctagttcgcc gacttgcttc ttgccgacga atatctggat
      721 gtggtcggtc cagtcgcgga ggtcgaggaa cacgaccttc ccctggccgc gccggagcat
      781 gatccgccca gcgacgcgga cggtcgggcc gggctgctcg tggccggcat cacgagccaa
      841 gtctgtggcc aacgcgcgcg cgacggaaat tgcctggtgc ccgtcaaatc gctgccccca
      901 cgggtcgacg ccgaggttgg cgattcgctc cagcttctcc acgcgcacgg cttccaggct
      961 ttcacgcgac tcgtccggca ttgctcccct tacttctttt cgaatagcca cttcgggcaa
     1021 acttgacaca aattgtagtg ttccgccatg tttggtcaac ggcaaccgtt atcaggacgg
     1081 ggctcctgac agtttgatct tgggcgggtg ggaaaagtgc cgtttgaacg caggcttgtt
     1141 ggtggctcgc gaatcgggca tttcgacaca aaccggtgct ttcccctccc aatgattttt
     1201 tgacagaaag aatggcacaa tttggcactc ctggaagtgg tctccgtgcc aatttgtcgc
     1261 tatcgcgatc gaagcctctt gcgcacaacc cttaattgac ttaaaagtta cgtcaaggcg
     1321 gtcttcacaa ggccgagggc gaaaaaagtg gcactcagag ccggttcgag ccagcagtgg
     1381 aggttgttta cgctacgatc gggcgaggtc gaaccgatca aggttcatca ctttgtccca
     1441 ggctgcgacg aagtcttcga cgaacttctt ctctgcgtcc gaacttccgt agacttcagc
     1501 cagggcgcgg agtatggagt tcgaaccaaa gatcaggtcg atacgcgtgc cggtccactt
     1561 cagttcgccc gttttgcggt cgcggccttc gaacacgtcc gcttctttcg agacggcctt
     1621 ccactccgtg ttcatatcga gcaaattcac gaagaagtcg ttgctaagcg tctccgggcg
     1681 cttggtgaag acaccgtggc ggactttggc ggcattggtg ttcaagacgc gcatgccgcc
     1741 cacgagtacc gtcatttcgg gcgcggtgag cgtcagcagt tgggccttat caatcagtaa
     1801 cgcttcggcc ggcacagagt aacggccctt gaggtagttg cggaatccgt cggcgatggg
     1861 ctcgagtacc gcgaaggatt ccacatccgt ctgctccagc gaggcgtcca tgcgccctgg
     1921 ggtgaagggc accgtcacgt cgtgacccgc tttcttggcc gcctgctcga ctgccgcaca
     1981 gccggccagc acgatcaggt cggccagcga cactttcttg ccgcccttct gcgcgccgtt
     2041 gaaggcactc tgaatcccct ccagtttctg aagcaccgtc gccagttggc caggcgtgtt
     2101 gacttcccag tccttctgcg gtgtcagccg aacgcgggca ccattcgccc cgccgcgctt
     2161 gtcggagcca cggtacgtgg atgcggatgc ccaagcagtc gacaccaatt gcgagaccgt
     2221 caggccggaa gccaggatct tgcttttcag cgaggcgatg tcctgcgcat cgatcaaggg
     2281 gtggttgact tcggggatcg gatcctgcca gatgagtttt tccgccggaa cttccggccc
     2341 gagataacgc gcacgcgggc ccatgtctcg gtgcgtcagc ttgaaccagg cgcgggcgaa
     2401 cgcgtcggcc aactcggcgg gattctcgaa gaaacgccgc gagattttct cgtaagtcgg
     2461 gtcgaatttc agggcgaggt cggtggtgag catggagggc gcgatccgct tggagggatc
     2521 atgcgcgtgc ggcacggtgc cggcgccggc gc
//
LOCUS       DATAQL010000912         6050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1009882, whole genome shotgun sequence.
ACCESSION   DATAQL010000912 DATAQL010000000
VERSION     DATAQL010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009882"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2934
                     /locus_tag="VHS97_15855"
     CDS             <1..2934
                     /locus_tag="VHS97_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX3449730.1"
                     /translation="GLEFPDPSIEILDGASRRDFLKFMGASLALGGIGGCAYQPAESI
                     VPYVDAPEAIIPGKPLYFASVIPIDGYACGVLVKSHMGRPINLEGNPAHPASLGAVDI
                     FGQAEVLTLYDPDRSQMVTHNARVDTWEHFQTLALDVREKLVPKKGAGLRVLTRTVAS
                     PTLADQLRRLGEQLPELKWHSYEPVTRSAIWAGSRLAFGEDLEPIHHLDRADVVLALD
                     ADFLARGPARLNNARGFATRREVNDPQSTASMNRLYVAEPMLSLTGAAADHRLAVTPG
                     EVALLTQTIAGLVGAVGSAAAEEYKIPVGLADNAPWLKAVARDLAAHRGKSLVIAGET
                     QPAEVHALVHLINQSLGNVGKTVDYIARVDAGPSDQLGSLRELVRDMNAGVVDTLIIL
                     GGNPAYDAPVDLEFAKVLASDKVKLRVHLGLYNDETAQLCHWHIPEAHCLESWSDILA
                     FDGTASIQQPLIAPLYKGKTAHELLAVFLGEPDKTGLQIVRDYWRRNLTGDFESRWRT
                     ALEDGLIASTTRQPKNVAPKIKEVTLPAAPATGGGDSLEILFRPDPTVGDGRFANNAW
                     LQELPKSLTKLSWDNAALVGPGLAQRLGLESEDVVVLQYQGRSARLPVWITPGQADGV
                     VTVFLGHGRRRAGKVGTGVGVDVYGLRTADQPWSGAGLEIKKTGDRYRMAAMHHHFSM
                     EGRDLIRVANLEEYRQNPAFAQEAEHEPGHGLSLFEDPGPQAQHEHGTGNAWGMAINL
                     NTCIGCNACVVACQAENNIPVVGRQQVLASREMHWIRIDRYFEGTDADLPKVDFQPVP
                     CMQCEKAPCEIVCPVGATTHSAEGLNEMTYNRCVGTRYCGNNCPYKVRRFNFLQYTDQ
                     NTPSLKLLRNPDVTVRSRGVMEKCTYCVQRINAARINAEIENRPVRGDEVVTACQASC
                     PTKAIVFGNLNDRDSAVVKAKASPRNYALLAELNTRPRTTYLAKLRNPNPEIEAKPRD
                     DSHRS"
     gene            2915..4336
                     /gene="nrfD"
                     /locus_tag="VHS97_15860"
     CDS             2915..4336
                     /gene="nrfD"
                     /locus_tag="VHS97_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX3449731.1"
                     /translation="MTPIDPESVYTRATAPILGPEHTYASVTDKISAIVLVRPYNWRW
                     FLGFGIGLALTALLIVSITVLLWNGVGIWGIDVPVMWGFAIVNFVWWIGIGHAGTLIS
                     AILLLLKQDWRTSINRFAEAMTLFAVACAGLFPLLHLGRPWLFYWLIPYPNTMGLWPQ
                     WRSPLIWDVFAVSTYATVSLLFWYVGLIPDLATLRDRSKNRWAQYVYGILAMGWRGSA
                     RHWHRYRTTYILMAGLATPLVVSVHTIVALDFTVAIVPGWHSTIFPPYFVAGAIFSGF
                     AMVMTLAIPLRAAFGVKDYITDRHLDNMAKVILATGLIVAYGYLMEAFMAWYGNNPYE
                     KFVQLKNRPTGPYAHTYWMMLTCNVFVPQLLWSRWVRGSVPLLFLISILVNVGMWLER
                     YVIVVTSLHRDFLPSSWSMYHGTFWDYSTYYGTLGLFLALIFLFIRFLPVISITEMRE
                     LVHETQHELERPGEALPPHGVTS"
     gene            4333..4881
                     /locus_tag="VHS97_15865"
     CDS             4333..4881
                     /locus_tag="VHS97_15865"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX3449732.1"
                     /translation="MSTKPETPVLYGIMAEFDDPDQLIEATQHAYDRGYRMMEAYSPF
                     PVDGLAEALGFHRNRIPRMVFLGGLFGGVGGFFMQWFSATIHYPLNIGGRPFNSWPAF
                     IPITFELTILCAALSAVLSMLWFNGLPRPHHPVFNVPNFALASRNRFFLCLQARDPLF
                     DPESARHFLEDHRPKAVDVVPW"
     gene            4940..5554
                     /locus_tag="VHS97_15870"
     CDS             4940..5554
                     /locus_tag="VHS97_15870"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3449733.1"
                     /translation="MFRSRGQTDGLALGQRIVLLFLVLPGCRSEMYDQPRFEPYEATE
                     FFDDGTSARPLVAGTVPRRDPREQGKSSDELFTTGKKAGQLADSLPFPVDSALLDRGQ
                     DRYRIFCTPCHGELGDGRGMIVQRGFNPPPSYHSEELRKKPVGHYFDVMTRGFGTMYS
                     YASRISARDRWAIAAYIRALQLSQHATAGDLSPEDRGQLQGVGP"
     gene            5551..>6050
                     /locus_tag="VHS97_15875"
     CDS             5551..>6050
                     /locus_tag="VHS97_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449734.1"
                     /translation="MSSNMTADSELQERFDRIQLNALVVGGLGLVVCALAWVIWPAQI
                     FPAYLVGFLYWIGISLGCIGLAMLHHLVGGSWGLVIRRPLESGALNVLPLAVLFLPVA
                     IGVKTLYPWAHDVSVGHDALIEHSPYLTESFFWIRAAVYFATWIAMALVLNGLSDRQD
                     ATTDPAP"
BASE COUNT         1096 a         1861 c         1860 g         1233 t
ORIGIN      
        1 ggcctcgagt ttcccgatcc atcgatcgag atactggacg gagccagccg tcgcgatttt
       61 ctcaagttca tgggcgcctc gctcgccctg ggaggaatcg ggggctgtgc ctatcagccc
      121 gccgagtcga tcgtgccgta cgtggatgcg cccgaagcaa tcatcccggg caaacccctc
      181 tatttcgcgt cggtgattcc gatcgacggc tatgcatgcg gagtgttagt caagagccac
      241 atggggcggc ccatcaacct cgagggaaac ccggcccatc ccgcgagttt gggggcagtc
      301 gacatcttcg gccaggccga agttttgacg ctttacgatc ccgaccgctc gcaaatggtg
      361 acgcacaatg cgcgggtcga cacctgggag cattttcaga cgctcgcgct cgacgtccgc
      421 gaaaagctgg tgccgaagaa gggggccggc ctgcgcgtcc tgacccgaac agtggcctcg
      481 ccgaccctgg ccgatcaatt gcgccgcctc ggggaacaat tgcccgagct caaatggcac
      541 agttacgagc ctgtgactcg cagtgccatc tgggctggaa gcaggttggc cttcggcgag
      601 gatctcgagc cgatccacca cctcgaccgc gccgacgtgg tcctggcgct cgatgccgac
      661 tttctcgctc gaggaccagc gcgattgaac aacgcccgcg gatttgcaac gcggcgcgag
      721 gtcaatgatc ctcagtcgac tgccagcatg aaccggctgt acgtcgcgga gccaatgctg
      781 agtctgaccg gtgccgccgc cgaccaccgg ctggccgtca cgcccggtga ggtcgctctg
      841 ctcacccaga ccatcgccgg cctggtcgga gcggtcgggt ccgcggcggc cgaagagtac
      901 aagattccgg tcggactagc cgacaatgcc ccttggctaa aggcggtggc gcgcgacctg
      961 gctgctcatc gcggcaagag tctggtcatc gccggcgaaa cgcaaccggc agaggttcac
     1021 gcgctcgttc atctgatcaa ccagtccctg ggaaatgtgg gcaagacggt tgactatatt
     1081 gcccgtgtgg atgccgggcc gagcgaccag cttggctcgc tgcgcgagct tgtccgcgac
     1141 atgaacgccg gggtggttga tacgcttatc attctcggag gcaacccggc ctacgacgcg
     1201 cccgtcgacc tcgagttcgc aaaagtgttg gcgagcgaca aggtcaagct cagggttcac
     1261 ctcgggcttt acaacgacga gactgcccag ctctgccact ggcatattcc cgaggcccac
     1321 tgcctcgaat catggagcga catcctcgcc ttcgacggca cggcgtcgat ccagcaaccg
     1381 ctgatcgcgc cgctctacaa agggaaaacg gctcacgaac tgctggcggt cttcctgggc
     1441 gagcccgata agacgggtct gcagatcgtc cgcgactact ggcgccggaa tctcaccggc
     1501 gatttcgaat cgcgttggcg cacggcgctc gaggacgggt tgatcgcgtc aaccactcgc
     1561 caacccaaga acgtcgcacc caagatcaag gaagtgacac tcccagcggc gccggcgacg
     1621 ggaggtggcg acagcctgga aatcctcttc cgtcccgacc cgacagtcgg cgacgggcgg
     1681 ttcgccaata acgcctggct gcaggaattg cccaagtcgt tgacgaaatt gtcgtgggac
     1741 aacgcggcgc tcgtcggccc cgggctcgcg cagcggctcg gtctggaaag cgaggacgtg
     1801 gtcgttttgc agtatcaggg caggtcagca cggctgccgg tctggatcac gcccggccag
     1861 gccgacggag tggtgaccgt ctttttggga cacggacggc ggcgggcagg caaggtcggc
     1921 acgggagtgg gcgtcgacgt ctatggtctg cgcacggccg accagccgtg gtcgggcgcc
     1981 ggcctcgaaa tcaagaagac cggcgaccgc taccggatgg ccgccatgca tcatcacttc
     2041 agcatggagg ggcgcgacct gattcgtgtc gcgaatctcg aggaatatcg ccagaatccg
     2101 gcgttcgcgc aggaggccga gcacgaaccc ggtcacggcc tgagcttgtt cgaagatccc
     2161 ggaccgcagg cgcaacacga gcatggcacg ggcaacgcct ggggcatggc aatcaacctc
     2221 aatacctgca tcggttgcaa tgcgtgcgtc gtggcctgcc aggctgagaa caatattccg
     2281 gtcgtcggcc gccagcaggt gttggcctcg cgtgagatgc actggatccg catcgatcgt
     2341 tactttgaag gcacggacgc cgacctaccc aaggtcgatt ttcagcccgt cccctgcatg
     2401 cagtgcgaaa aagcgccgtg cgagatcgtc tgtccggtcg gggcgacgac gcacagcgct
     2461 gaggggctca acgagatgac gtataaccgg tgcgtcggca cccgctattg tgggaacaac
     2521 tgcccgtaca aagtgcgacg cttcaatttt ctccagtaca ccgatcagaa cacgccgagc
     2581 ttgaaattgc tgcgaaaccc ggatgtgact gtccggtcgc ggggcgtcat ggaaaaatgc
     2641 acatattgcg tgcagagaat caatgcggcg cggatcaacg ccgagattga aaatcgtccg
     2701 gtgcgcggtg acgaggtcgt gacggcctgt caggcgtctt gcccgaccaa ggcgatcgtg
     2761 tttggcaact tgaacgaccg cgacagcgcc gtcgtcaagg cgaaagcgag tccccgcaac
     2821 tacgcgctcc tggccgagct caacacgcgg ccgcggacca cgtatcttgc caagctgcgt
     2881 aatcccaacc ccgagatcga ggcaaagccg cgcgatgact cccatcgatc ctgaatcggt
     2941 ctacacacgg gcgaccgcgc cgatcctggg gccggagcac acctatgcgt cggtgaccga
     3001 caagatcagc gcgatcgtgc tggtgcgccc ctacaactgg cgctggtttc tcggctttgg
     3061 catcggtttg gcactgacgg cgctcttgat cgtgtcgatc accgtgcttt tgtggaacgg
     3121 cgtgggcatc tgggggatcg acgtgccggt gatgtggggc ttcgcgatcg tcaacttcgt
     3181 ctggtggatc ggtatcggcc acgccggcac cttgatctcg gcgattctcc tcttgctcaa
     3241 gcaagactgg cgcacctcga tcaaccgatt cgccgaagcg atgacactat tcgccgtcgc
     3301 ttgcgcgggc ttgttcccgc tcttacacct gggacggccc tggctttttt actggctgat
     3361 tccctatccc aacacgatgg ggctctggcc gcagtggcgc agccccttga tctgggacgt
     3421 gttcgccgtg tccacgtatg ccacggtctc attactattc tggtatgtcg ggctgattcc
     3481 cgacctggcg accttgcgcg accggtccaa gaatcgctgg gcgcaatacg tctacggcat
     3541 cctggccatg gggtggaggg ggtcggctcg gcactggcat cggtaccgca ccacgtacat
     3601 cttgatggcc ggattggcga ctccactggt cgtctcggtg catacgatcg tggccctcga
     3661 cttcaccgtc gcgatcgtgc ccggctggca ctcgaccatc tttcccccct atttcgttgc
     3721 gggcgcgatc ttctcgggat ttgccatggt gatgaccctc gcgattcctc tgcgcgcggc
     3781 ctttggcgtt aaggactata tcacggatcg ccacctcgac aatatggcca aggtcatttt
     3841 ggcgaccggc ctgatcgtgg cctacggcta tttgatggaa gcgttcatgg cgtggtacgg
     3901 caataatccc tacgagaagt ttgtccagct caagaaccgg ccgaccggcc catatgcaca
     3961 cacgtattgg atgatgctga cgtgcaacgt gtttgttccc cagcttctgt ggtctcgttg
     4021 ggtccgcggc agcgtcccgc tcttgttttt gatttcgatc ctggtgaatg tcggcatgtg
     4081 gctggagcgt tacgtgatcg tcgtgaccag cttgcatcga gattttctgc cgtcatcgtg
     4141 gtcgatgtat catgggactt tctgggacta ctcgacttat tacggcaccc ttggtctctt
     4201 cctggctttg atatttctct tcattcgttt cttgccggtg atctcgatca ccgaaatgcg
     4261 cgagttggtg cacgaaacgc agcacgaact ggaacggccg ggtgaagcgc ttcctccgca
     4321 cggggtgacg tcatgagtac caagcccgag acaccagttc tctatggaat catggccgag
     4381 ttcgatgacc ccgaccaact gatcgaggcg acccagcatg cctacgatcg cggttatcgg
     4441 atgatggaag cgtattcgcc tttccccgtc gacggcttgg ccgaggcgct ggggttccac
     4501 cgcaaccgga ttccgagaat ggtgttcttg ggggggctgt tcgggggggt aggcggcttt
     4561 ttcatgcagt ggttctcagc gacgatccac tatccgctca acatcggcgg acgtccgttc
     4621 aatagctggc cggcgttcat ccccatcacc ttcgagctga cgattctatg cgctgcattg
     4681 tcggccgtgc tgtcgatgct gtggttcaac ggactaccgc ggccccatca tccggtcttc
     4741 aacgtcccca actttgccct ggcatcgcgc aaccgatttt tcctctgcct gcaggcccgc
     4801 gacccgttat tcgaccccga atccgcgcgg cacttcctgg aggaccaccg accgaaggca
     4861 gtcgacgtcg tgccatggtg aatgtcgtcc cgaccacaag gtgatcccta gaatgagccg
     4921 aggtagagta agccgcagta tgtttcgatc aagagggcag accgacgggc tggcgctggg
     4981 ccaacgtatc gtcttgcttt tcttggttct gcccggctgc cggagcgaga tgtacgacca
     5041 acctcgattt gaaccgtacg aggccactga attcttcgat gacggcacct cggcgaggcc
     5101 cttggtggcg ggcacggtcc cgcgccgcga tcctcgagag cagggcaaga gttccgacga
     5161 gctcttcacg accggcaaga aagccggcca gcttgccgac tcgctgccgt ttccggtgga
     5221 tagtgcgctg ctcgaccgag gccaggatcg ataccggatc ttctgcacgc cctgccacgg
     5281 cgagctggga gacggccggg gcatgatcgt tcagcgcgga ttcaatccgc ctccttcata
     5341 tcacagtgaa gagctgcgga agaaacccgt cggccactac tttgatgtga tgacccgggg
     5401 ctttggaacc atgtattctt acgcgtcgcg tatctcggcc cgcgaccggt gggcgatcgc
     5461 ggcctacatc cgggcactgc aactaagtca acatgcgacg gccggagacc tgtcgcccga
     5521 agatcgcggc cagctccaag gagtcgggcc atgagcagca acatgacggc cgattccgag
     5581 ctccaagagc ggttcgatcg aattcagttg aatgccctgg tcgtgggtgg tttaggactc
     5641 gttgtctgcg cgttggcatg ggtgatctgg ccggcgcaga tttttcccgc ctacctggtc
     5701 ggatttctct actggatcgg aatcagcctg ggttgcatcg gcctcgccat gctccatcac
     5761 ttggtgggtg gctcgtgggg acttgtgatc cggcgcccgc tcgaatcggg cgcgctcaac
     5821 gtgttgccgc tggcagtgct attcttgccg gtcgcgatcg gagtcaagac actctatccg
     5881 tgggcgcacg acgtctccgt cgggcatgac gctctgatcg agcacagtcc gtatctgacc
     5941 gagtcgtttt tctggattcg cgcggctgtc tattttgcga cctggatcgc gatggcgctg
     6001 gtcttgaacg ggctctcaga tcgccaggac gccaccaccg atccggcgcc
//
LOCUS       DATAQL010000913         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2995094, whole genome shotgun sequence.
ACCESSION   DATAQL010000913 DATAQL010000000
VERSION     DATAQL010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2995094"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..572
                     /gene="terL"
                     /locus_tag="VHS97_15880"
     CDS             111..572
                     /gene="terL"
                     /locus_tag="VHS97_15880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEX3449735.1"
                     /translation="MNFAGRGVVVNQPDGDKIMRLHAQTTVIEAGFVYVPETAPWLAE
                     YLHEMTVFPKGKHDDQVDSTAQFLDWFKKPFPSQGLFEYYRMLAEKLQRRTRTWVRLR
                     SPYGTGSVQTFSGRRLNFGPDGTVEMSAEDAEHLIRGGWTKLAEWTSDEAV"
     gene            1061..>2098
                     /locus_tag="VHS97_15885"
     CDS             1061..>2098
                     /locus_tag="VHS97_15885"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX3449736.1"
                     /translation="MNTGGAPIRGPAGRRFSITVQLLIGVAAAAGASVPAAAQEAPLA
                     NPPELTVLRGPAVPAPMTTMAHEAAPVERVGNEARLELDVTYGYYRIWNASTKRYDVV
                     RLRSYNGTVLAKPADASGWKDAPDPVGPTIRLKPGETVRATLSNQLPTKPCVSPEGTP
                     NIPNCFNTTNLHSHGLHVTPTGNGDNVMLEVRPGETFQYEYNLPFDHPAGTFWYHAHR
                     HGSTALQVSSGMAGALIIEGDRLPAKQGEAWLPGDIDTILRAGDKPFPERILLFEQIQ
                     YACRDPSGQIKVIKNRDGQVQAWKCDPDDVGGVEGYDQFGPGTWTQSGRYTILNGRLQ
                     PALAAAKVGQIE"
BASE COUNT          389 a          692 c          673 g          344 t
ORIGIN      
        1 gccttcgtat ttgcgaatga tctgatcgaa aaaggccggc gccaggttgc cgcgattttc
       61 gtagctcgag ccgcgggtca cgacgcagat cgggtcgcgc accagcgcgg atcaatttcg
      121 cggggcgcgg cgtcgtcgtt aaccagccgg atggcgacaa aatcatgcgg ctgcacgcac
      181 agacgacggt gatcgaggct ggcttcgtct acgtccccga aacagccccc tggctcgccg
      241 agtatctcca cgagatgacg gtcttcccga aaggcaaaca cgacgaccag gtcgactcga
      301 cggcgcagtt cctcgattgg ttcaaaaaac ctttcccgag ccaggggctc ttcgaatact
      361 accgcatgct ggccgagaaa ctccaacgcc ggacgaggac ttgggtgcgc cttcggtcgc
      421 catacggcac cggatccgtg caaacctttt cgggccgccg cctcaacttc ggcccagacg
      481 gcactgtcga gatgtctgcc gaggatgccg agcacctgat ccgtggcggc tggaccaaac
      541 tcgcggaatg gaccagcgac gaagcggtgt gatcggcatg ccgctcacca ggcagtcggc
      601 ccaggtgtcg actttgacgg gggaccgaga gttcgaatcc gccttcctcc agcggcgagt
      661 ctcgtgcgaa cctgtctttc ttcgattaga gcgccaaaaa tttgccattc gcgaagtctc
      721 ctgcgaactt gttatggaac cagataccgc acgaatcagc ggcattcagc ccgaggatgc
      781 gatggatcaa acgcgtcgcc aaaatcgacc tctcacccgt tcagtcgggc ttgtcgtccc
      841 cgaatggggc cggtttcggt cgggcacatg ggaaatgtcg gttagggccg cggcgagcgc
      901 gcgggctggg ggaaaaatat cgagaggaga tttcctctac agaacttccg ccgggagttg
      961 ccatctcgag gttgctctgc catggttaac tcgctgccga cctcctgtga cggccctcgg
     1021 tggaggccgc ggggcgggat ccggcaagac gggggtcgca atgaacacgg gcggagcacc
     1081 gatccgcggg cctgccgggc ggcgtttttc cattacggtc cagcttctca tcggcgttgc
     1141 ggccgcggcc ggcgcttccg tgccggccgc ggcccaggag gcgccgctcg ccaatccgcc
     1201 cgagctcacg gtgctgcgtg ggccggcggt gccggcgccc atgacgacga tggcgcatga
     1261 ggcggcgccg gtcgagcgcg tcggcaacga agcgcggctc gagctcgatg ttacctatgg
     1321 ctattaccgg atctggaatg cctcgaccaa gcgctacgac gtcgtgcgct tgcgctccta
     1381 taacggcacg gtgctggcca agccggcgga cgcgtcgggc tggaaggacg cgcccgaccc
     1441 ggtcgggccc acgatccgcc tgaagcccgg cgagacagtg cgcgcgacgc tcagcaacca
     1501 gctgccgacc aagccctgcg tcagcccgga gggcacgccc aacatcccga actgcttcaa
     1561 cactaccaat ctgcactcgc acgggctgca cgtcacgccg accggcaacg gcgacaatgt
     1621 catgctggag gtccggccgg gcgagacctt ccaatacgag tataatctgc ccttcgacca
     1681 cccggccggc accttctggt accacgccca tcgccacggc tcgaccgcgc tgcaagtgtc
     1741 gagcggcatg gccggcgcgc tgatcatcga gggcgaccgc ctgccggcca agcagggcga
     1801 ggcctggctg cccggcgata tcgacacgat cctgcgcgcc ggcgacaagc ccttccccga
     1861 gcgcatcctg ctgttcgagc agatccagta cgcctgcagg gatccgagcg ggcagatcaa
     1921 ggtgatcaag aaccgcgacg gccaggtcca ggcctggaaa tgcgatcccg acgatgtcgg
     1981 cggcgtcgag ggttacgacc agttcggccc cggcacctgg actcagtcgg gccgctatac
     2041 gatcctcaac ggccggctgc agccggcact ggccgcggcc aaggtcgggc agatcgaa
//
LOCUS       DATAQL010000914         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3911898, whole genome shotgun sequence.
ACCESSION   DATAQL010000914 DATAQL010000000
VERSION     DATAQL010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911898"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1597)
                     /gene="rny"
                     /locus_tag="VHS97_15890"
     CDS             complement(74..1597)
                     /gene="rny"
                     /locus_tag="VHS97_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX3449737.1"
                     /translation="MVTGVLIGLLSAYLITRLRAQTARGLAEHILANANLQAETIRRQ
                     AELAVKEEVLKRREDLEAEIDRVRRDLRDQERRLEKRADTLDQKLDLQNKKEIEFVNQ
                     GRLLAEQQEEVRQRQVEVTQALAAQLETLQSISQLSANDARELLLKRVEEELRGEVGS
                     LVLKYQTTVHDACQQKSREILTTAIQRYAASHTAEITVSTVDIPNDEMKGRIIGREGR
                     NIRAFEKATGVDVIVDDTPGVVIVTGFDSIRREVAKIALERLIQDGRIHPTRIEEIVK
                     ETQEDMEQHVRRLGQEAALEANILGLHDKLLDYLGKLHFRTSYSQNVLRHSLEVAYLT
                     GLLAEEIGLNGPLGRRCGLLHDIGKAADHEMEGGHPLIGAELTRRYGESKEVIHAALG
                     HHDDLRVETPYTMLVSAADAISASRPGARRETLEKYVRRLEELESLALGFLGVDQAYA
                     IQAGREIRVLVDSRQLDDAESAKLCRDIAQSIQTRLSYPGEVKVTVLRETRSVEFAR"
     gene            complement(2453..2872)
                     /locus_tag="VHS97_15895"
     CDS             complement(2453..2872)
                     /locus_tag="VHS97_15895"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3449738.1"
                     /translation="MTERSQRRLESILLSSWFVREDKPIRIPDFDEPRPDLAVVRGAT
                     ERYAHHHPGPADVALLIEVSDASLQRDQGDKLMIYAQAGIPVYWIANLVNRRVRLYGD
                     PGPGGYASRVSFGSGQNIPVVIGGVHVGTVGVDSVLG"
BASE COUNT          685 a         1043 c         1005 g          711 t
ORIGIN      
        1 gacgcctggg agactgactt tatctcccgc atggcgcgaa ccggagtaac acgatctcga
       61 aacgaaccat ccttcaccga gcgaactcca cgctgcgcgt ctcgcgtaga acggtcactt
      121 tcacctcgcc gggataagac aggcgagttt ggatcgactg agcgatatcg cggcaaagct
      181 tggcggactc ggcgtcgtcg agctgccggc tgtcgaccag gaccctgatt tcgcggccgg
      241 cttggatcgc gtacgcctgg tccacaccca ggaatcccaa ggcgagcgat tcgagctctt
      301 ccaggcgacg gacgtacttc tcgagagttt cgcgccgcgc gcccggtcgg ctggcactga
      361 tcgcgtcggc cgcggaaacc agcatggtgt acggcgtctc gacgcgcaaa tcatcgtgat
      421 gcccgagcgc cgcatgaata acctcctttg actcgccgta gcggcgagtc agctcggctc
      481 cgatcaaggg atggcctcct tccatctcgt gatcggccgc cttgccgatg tcgtgcaaaa
      541 ggccgcaccg ccgcccgagt ggcccattca agccgatttc ctcggccagg aggcccgtca
      601 aatacgcgac ttccaacgaa tgcctcagca cgttctgcga ataactggtg cggaaatgca
      661 gcttgccgag atagtccagc agcttgtcgt gcaggccgag aatgttggcc tcgagggcgg
      721 cctcttgtcc caggcggcga acgtgctgct ccatgtcttc ttgcgtttcc ttgacgattt
      781 cctcgatccg ggtgggatgg atgcgaccat cctgaatcag ccgttcgaga gcaatcttgg
      841 cgacctcgcg gcggatgctg tcgaaaccgg tcacgatcac cacgccgggc gtgtcgtcga
      901 cgatcacgtc gacgccagtg gccttttcga aggcgcggat attgcgcccc tcacggccga
      961 tgatccttcc tttcatctcg tcgttgggga tgtcgacggt gctcacggtg atttcggccg
     1021 tgtgcgacgc ggcgtagcgc tggattgcgg tggtgaggat ctcgcggctc ttttgctggc
     1081 atgcgtcgtg aaccgtagtc tggtatttca aaaccaagct tcccacctca ccgcgcaact
     1141 cttcctcgac gcgcttcaag agcaattcgc gcgcgtcatt ggcggaaagt tggctgatgc
     1201 tctgcagggt ttcgagctga gcggcgagcg cctgtgtgac ctccacctgg cgctggcgca
     1261 cttcctcctg ttgttcggcc agtaatcgcc cttggtttac gaactcaatt tcttttttgt
     1321 tctgcagatc aagcttttgg tcgagcgtgt ccgcacgttt ctcgagtcgg cgttcttggt
     1381 cgcggagatc gcggcggact cgatcgatct ctgcctcgag atcctcacga cgcttcaaga
     1441 cctcttcctt gactgccagc tcggcctgtc ggcggatcgt ctcggcttgg aggttggcat
     1501 tggccaggat gtgctcggcg agaccgcgcg cggtttgagc ccgcaaccgc gtgatcaggt
     1561 acgcgcttaa gaggccgatc agtacgcctg ttaccaaccc gacgtagccg ctcaggggaa
     1621 ctcctccagg gcgcggtgcg accttatcgc gagctggccc gtagcgcgat caatctcaca
     1681 ggaggagcgg acgagaaagt gtgccggcgc cgacaacggc tcaaacgatt agccaaagac
     1741 ctcgccatgc caggcgcggt ctcccagtcc ggcaggaaat atcggataga gggcctgaag
     1801 cgtgaagaaa tttcaccccg cgctcgcgga accgccgaca gtgtgcgcct gacaaatcga
     1861 acggtggagt ccaacacaaa gaccacgggt tgttacgccg gtgtggcgaa cacccagctt
     1921 tcatgtccgc gaactgatat ttaatcaggc tggaacccgg ccggatccgc gggcgaagga
     1981 agcgccgagc gcaacctcct tcgaaccgcg gaaagaccga cccgactcgg cttgatacga
     2041 tcacccaaag ggtggtcagc atcgagatca gcccttggct aaacttcgtc acaccagtga
     2101 tcctcgtgac cgtctggttt cgtttggatt cgttctcggc gccggacttc cacgaccgcc
     2161 tcggcctgtc tgtcagactg atgacgagga agacgcgtta gaaagcccgg acatcattga
     2221 aacttcactt ggggaacgcc cacccgtgtt cgaccgatcg caattgcaaa ggcgaagacg
     2281 ttccggaacg taactccacg ttcaaacgag cgtggattag tcgtctaatg tatcagaaga
     2341 gtttcgcagg tcaattggcc ggcgacgggt ctcactgggc gcggcgaaat ccctgccgat
     2401 ctaacagggc caatacgcgt caaggaagtc gcggattcgg actttctgag ctctagccga
     2461 ggaccgaatc cacaccaact gtgccgacat gaacgccgcc gatgacgacg gggatgttct
     2521 gccccgatcc gaagctgacg cgagacgcat atccgcccgg gccaggatct ccgtagagcc
     2581 gtacccgccg attaaccagg ttggcgatcc agtaaaccgg aatcccggct tgggcgtaaa
     2641 tcatgagttt atcgccctga tcgcgctgga gactcgcatc cgatacctcg atcaggagcg
     2701 caacatcggc cggtcccgga tgatgatggg cgtacctttc agtagcgccg cggacaacgg
     2761 cgagatccgg tcgcggctca tcgaagtctg ggattcgaat tggcttgtcc tctcgaacaa
     2821 accagctgga taaaaggatc gactcgagcc gtctctgcga ccgttccgtc acgatgacgt
     2881 gcgacccttc atgtcttccc tcccgacgat gtatccgttg atcaactcga cctggtcgtc
     2941 atcgagtgag ttcgcaatcc gctcgaactc gtctactgtc attcggtaca acgccggagg
     3001 cgctggcatc gattgcgatg gagtgatcag ggacattgga catccgctcc gcttgaggac
     3061 gttcttcacg gcgtggggct cggtcgcact cagaccggca cttctacatt gcgatagctc
     3121 gagcgatagt gaaagtgcac acgctccaac cgatttggct caccgcggtt gctgttcccc
     3181 ggccggctcg atcgctcgta ggagtgctct ttgcgagaac cattttacga ccgtacaatc
     3241 aacttggtgg aacagcacgg gtccaaacgg agaagaaatc gagcgtgacg ctacagacct
     3301 tccgagattt gcttgcggca agaccgttca agccgttccg cctggtaatg tcgagaggcc
     3361 agacttacca cgtacgcaat cccgaaatgg cgatgcacac ccgaacggat ttgcttgtcg
     3421 gagtcgacat cacggacgac ggcg
//
LOCUS       DATAQL010000915         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2690726, whole genome shotgun sequence.
ACCESSION   DATAQL010000915 DATAQL010000000
VERSION     DATAQL010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690726"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VHS97_15900"
     CDS             complement(<1..745)
                     /locus_tag="VHS97_15900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3449739.1"
                     /translation="MSIDLSVLIPVFNEVDNVEPLHAELDVVLRTLPLRYELIFVDDG
                     STDGTGSRLEAIQGCDHDHVRVAFLRRNCGQTAALSAALDLARGEILVPIDGDRQNDP
                     ADIPRLMKEIEAGYDVVSGWRKDRQDAFLTRKVPSWIANRVVGRLSGVLLHDFGCTLK
                     AYRRRVLDGVRLYGEMHRFIPIFAAWQGARVTELVVNHRPRTAGRTKYGLGRTFHVIL
                     DLILIRFLQRYAQRPLHFFGQFGLWSFGLA"
     gene            complement(831..1748)
                     /locus_tag="VHS97_15905"
     CDS             complement(831..1748)
                     /locus_tag="VHS97_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3449740.1"
                     /translation="MKLGFVSAILPEYTLDQVLSFASASGFSSVELMCWPPGKAERRY
                     AGVTHFDATNLSARALATVRELLAAHPVSISGLGYYPNPLAADRAEAQAGVTHLHAVI
                     EAAAALGVGVVNSFVGRDPALSVEANWPRFLEVWRPLIAHAEDKSVKIAIENCPMFFT
                     SDEWPGGKNLATTPAIWRRMFDDISSPNFGLNFDPSHMIWQQMDYIEPLTEFKDRIFH
                     VHAKDARIDHRALDQHGVMSYPKLWHTPKIPGLGDVRWGAFFAGLCDAGYNGHVVIEV
                     EDRAFEGSLESRLESLVISRRYLLQYCGG"
     gene            complement(1840..>2428)
                     /locus_tag="VHS97_15910"
     CDS             complement(1840..>2428)
                     /locus_tag="VHS97_15910"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3449741.1"
                     /translation="PLTMALVAICVVVFVFQRSREGRSLERKLWFSNTQVSATGGEKD
                     SGLSDILHGELWRLVTPIFMHGNPIHIFFNLWWLVSLGTMIEARRGTLRLAGLVLIAA
                     IVSNFGQYIWMERIYPGEPHRFLGMSGVVYALFGYIWMKSYYEPEQGLFMHPNNVNLM
                     LLWLVLCMTGVVGHIANAAHFVGLMVGVALAALRF"
BASE COUNT          588 a          738 c          664 g          438 t
ORIGIN      
        1 cagccaaacc aaacgaccat aagccgaact gcccgaagaa gtgcaggggc cgctgtgcat
       61 aacgctggag aaaacgaatg aggatcaagt ccagaatgac gtgaaaggta cgtcccaatc
      121 catacttggt ccgtccggcc gtgcgcgggc gatgattgac gaccagctcg gtcacccgcg
      181 cgccttgcca tgccgcgaat atggggataa aacggtgcat ctcaccatag agacgcacgc
      241 cgtccaagac acgccgtcga taggctttta atgtgcagcc gaaatcgtgc aacagcacgc
      301 ccgagagccg gccgacgacc ctgttggcga tccacgacgg caccttgcgg gtcaagaagg
      361 cgtcttgacg atcctttctc caacccgaga cgacgtcgta accagcctcg atctctttca
      421 tcaaccgggg gatgtccgcc ggatcgtttt gacggtcgcc gtcgatggga accaagatct
      481 cgccgcgggc aaggtcgagg gcggccgaaa gagcggccgt ctggccgcaa ttccgcctga
      541 gaaacgcaac ccgaacgtgg tcgtggtcgc acccttgaat cgcctcgagc ctcgagcctg
      601 ttccatcggt agaaccgtcg tcgacaaaga tcagctcgta gcgcaatggc agcgtgcgca
      661 acaccacatc aagctcagcg tgcagcggct cgacattgtc gacttcgttg aacacgggaa
      721 tcaaaacgga gaggtcaatc gacatcaaaa tcaaagatgg ctagttgcaa gtatcaggca
      781 aaaacagacc agacactctc cactatctcc atgctcctgg caaatcgcaa ctaaccgcca
      841 cagtactgaa gtagataccg gcggctgatc acgagcgatt cgagtcgcga ttcgagcgag
      901 ccctcgaatg cgcgatcctc gacctcgatc acgacatggc cgttgtagcc cgcatcgcag
      961 aggccggcga agaaggcacc ccagcgaacg tcgcccaagc ctggaatctt gggcgtgtgc
     1021 cataacttgg gataagacat cactccatgc tgatcgagag cgcggtggtc gatcctcgcg
     1081 tctttggcat gaacgtggaa gatgcgatcc ttgaattccg ttagcggttc gatgtaatcc
     1141 atctgttgcc agatcatatg cgatgggtcg aagttcaaac cgaagttcgg gctcgaaata
     1201 tcgtcgaaca tcctccgcca gattgcaggc gtcgtcgcca gattcttgcc accgggccac
     1261 tcatcgctgg tgaagaacat cgggcaattc tcgatggcga tcttgaccga tttgtcctcg
     1321 gcatgagcga ttagcgggcg ccaaacttcg agaaaacggg gccagttggc ttcgaccgag
     1381 agagccggat ctcggccgac gaatgaattg acgacgccga cccccagcgc cgcagcggcc
     1441 tcgatcaccg catgaaggtg cgtcactccc gcttgggcct cggcccgatc ggccgccagt
     1501 gggttgggat aatacccaag cccggaaatc gaaaccgggt gagccgccag gagctctcgc
     1561 accgtcgcga gcgcacgcgc ggacaggttc gtcgcgtcaa aatgagtgac ccccgcgtac
     1621 cgccgctcgg ctttgcccgg cggccagcac atgagctcga cgctcgaaaa gcccgacgcg
     1681 ctcgcaaaac tcagcacctg atcgagcgta tattccggca agatagctga cacaaaccca
     1741 agtttcatga acttccctct cgttttggtc aattacacaa cttcatatcg atgtcggttt
     1801 ccggctgacc tgaactcgag tgcacgccag gcatcccgcc taaaacctca acgcagcaag
     1861 ggcgacgccc accatcaaac caacgaaatg cgcggcattg gcgatgtgac cgacgacacc
     1921 ggtcatgcaa agcactagcc agagaagcat gagattcacg ttattgggat gcatgaagag
     1981 cccctgctcg ggttcgtaat aactcttcat ccagatgtac ccgaacaggg cataaaccac
     2041 ccccgacatg cccaggaacc tgtgaggctc accggggtaa attcgttcca tccaaatata
     2101 ctggccaaag ttcgacacga tcgctgcgat caagaccaac ccggcaagcc ggagggttcc
     2161 acggcgggcc tcgatcatgg tccccagcga gacgagccac cacaaattaa agaaaatgtg
     2221 gatggggttt ccatgcatga atatcggcgt cacgagtcgc caaagttcgc cgtggaggat
     2281 gtcgcttagg ccgctgtctt tttcccctcc ggtcgcgctc acctgagtgt tggagaacca
     2341 cagcttacgt tccagcgagc ggccctcgcg tgatcgctga aacacaaaaa cgaccacaca
     2401 gatggccacg agcgccattg tcaacggg
//
LOCUS       DATAQL010000916         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4063421, whole genome shotgun sequence.
ACCESSION   DATAQL010000916 DATAQL010000000
VERSION     DATAQL010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4063421"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(452..1030)
                     /locus_tag="VHS97_15915"
     CDS             complement(452..1030)
                     /locus_tag="VHS97_15915"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449742.1"
                     /translation="MRETDLDATLSGEDHLGTAEAYNKLAANLNRQGRLADAELLYRE
                     AVDVRRKVSGEDHPDTGRAWNDLTVSLELQGRRDEAESLYRNAPGVFRKALGEDHPDT
                     AAAYYNPGFTLYALGKVPAATDNLTAAVKSFEGGRLAMSRSGLGRSQADRIDPLPALA
                     IARASAGLARVAEWTCIALPTASSLSRESRRR"
     gene            complement(1372..1590)
                     /locus_tag="VHS97_15920"
     CDS             complement(1372..1590)
                     /locus_tag="VHS97_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449743.1"
                     /translation="MTRPMPSYQLHALWLTLSLATLLLAPGCGSEAIPIASVPADATE
                     KVEKGQTKPVAKSDRLPPDREQSKGRPY"
     gene            complement(1628..2788)
                     /locus_tag="VHS97_15925"
     CDS             complement(1628..2788)
                     /locus_tag="VHS97_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449744.1"
                     /translation="MRRLYDLARPQPRGGFTLIELLVVIAIIAVLIALLLPAVQSARE
                     AARRIQCTNNLKQLGLALQNYHSSFANFPLGALQVRDGTGALRGTDWGLWSAQAMLLP
                     YLEQATLYNACNFAVPNQGNAGIDTTSMQVTAITTVVNGFLCPSSPPLNAPGFPPESP
                     ATLQTNSPGNNYFASIGSSLNQYSGPYISGQEMGGAASPNGMFEVFGQALTMTSVQDG
                     TSNTIAFGEWRTGDFNANVLSIQDVIDVGGLFPPGSGRYQLNQTMPYGGAGLNAWLQM
                     CAQKASGSLGNANLNRSWIGQQWCTGMFGRTLGNTVVAPNSNYPNCATVIWGSDFDGS
                     NGNFGMSSYHPGGANVAMVDGSVHFLKSSTSQPVVWALGSRAQGEISSADSY"
BASE COUNT          623 a         1116 c         1215 g          739 t
ORIGIN      
        1 gggttcataa cgactccttg agcgagagag caagattgtc gtgttcccgc acatcgtcga
       61 ctcaacgcat cgacgatcca tgccgccgcg agggcgcaac tgatctccga tggcatcagc
      121 ggcagctctc agtgtggcag actctccggc gccaacttcc ggtagggctc catttggacc
      181 tcccatattg taccttttgc catcgcggta taacgcgaat gaagcaaagg acatgcaacc
      241 catgccgcca actaccgaac tccttagccg gaattttttc gacgacagtt acttgtggat
      301 cctcgccagc gagaagcaac gacaggcggc gcgactatac aacagcccgg gaacgcgcta
      361 cggcccgagc tcgaatactc cgttttgcgc tcggcgattt gccaagcgcg agcatcgtcc
      421 gctgatccga atcggttcgc ccgtaaacgg gttagcgtcg tctagattct ctgcttaagc
      481 tcgatgcggt tggcaatgct atgcatgtcc attcagccac gcgggccagg ccggcggacg
      541 ccctggcgat tgccaaggcg gggagcgggt cgattcggtc ggcctgcgat cggccgagcc
      601 ccgagcggct catagccagc cggcccccct cgaagctctt gaccgcggcc gtcaagttgt
      661 ctgtcgccgc ggggaccttg cccagcgcgt atagggtgaa ccccgggttg taatacgccg
      721 ccgcggtgtc ggggtggtcc tcgccgagcg ccttgcggaa gacccccggg gcgttccgat
      781 acagcgactc ggcctcgtct cgacgcccct ggagctccag gctcaccgtc aggtcgttcc
      841 aggccctgcc ggtgtcgggg tggtcctcgc ccgagacctt ccgccggacg tccaccgcct
      901 cgcggtagag gagctcggcg tcggcgagcc gcccctgtcg attgaggttt gcggccagct
      961 tgttgtaagc ctcggcggtg ccgagatggt cctcgcccga gagtgtcgca tcgagatcgg
     1021 tttctctgat ccttcgcccc gaagggcgga atgaatcccc aaccccggca tccttgctat
     1081 agactccttt tcacggtctt ttatcgtccc gcgcccgagt agccggtttc aactagagtt
     1141 ggagcgatca gtagggtgcg ggacaacgcc gcagtctgcg gcgaggttaa gtcgccgacg
     1201 cgacccgttt ccttcgcttg agccgagcga gtgtggcgtt gctcgttttg tggcgtagac
     1261 tgaagttgcc gctggctagg ccggtcgggc ctgtatccca aggggctgag tgcacccggc
     1321 ccgcacttcc tccagtctca gtccctggcg aatgccctcc ggaaggcggg ctcagtaggg
     1381 acgacctttg ctctgctctc ggtccggcgg caggcggtcg gactttgcga ccggcttggt
     1441 ctgtcccttc tccaccttct cggtcgcgtc ggccggtacc gaggcgatcg gaatggcttc
     1501 gctaccacaa cccggcgcca gcaacagggt ggcgagcgat agggtcagcc agagggcgtg
     1561 caactggtac gatggcatcg gtcgtgtcat cggttcgagc tccgtggtga gtgatcggga
     1621 agagggctca gtacgaatcg gccgagctga tctcgccctg ggcccgcgag ccgagcgccc
     1681 acacgaccgg ctgggacgtt gaggacttga ggaagtggac ggagccgtcg accattgcga
     1741 cgttggcccc gccggggtga tagctgctca tgccgaagtt gccattggag ccgtcgaagt
     1801 cgcttcccca gatcaccgtg gcgcaattgg ggtaattcga gttgggggcg acgacggtgt
     1861 tgcccagggt ccggccgaac atcccggtgc accactgctg tccgatccaa ctccggttca
     1921 ggttggcgtt accgagcgac cccgaggctt tctgggcgca catctggagc caggcgttca
     1981 gcccggcgcc gccgtacggc atcgtctggt tgagctggta acggcctgat ccgggcggga
     2041 agagtccgcc gacgtcgatc acgtcttgga tcgacagcac gttcgcgttg aagtcaccag
     2101 tgcgccattc gccgaaggcg atcgtattgc tggtcccgtc ctgcacgctg gtcatcgtga
     2161 gcgcttggcc gaagacttca aacatgccgt taggcgatgc cgcgccgccc atctcctgtc
     2221 cagagatgta cgggccgctg tactggttga ggctcgagcc gatcgacgcg aaataattat
     2281 tccccggcga gttcgtctgc agggtggccg gtgattcggg tgggaacccc ggggcattca
     2341 gcggcggcga cgacgggcag aggaagccgt tgacgactgt ggtgatcgcc gtcacctgca
     2401 tcgacgtcgt gtcgatcccc gcgttcccct ggttggggac ggcaaagttg cacgcgttgt
     2461 acagcgtggc ctgctcgagg tagggaagga gcatcgcctg ggcgctccat aggccccagt
     2521 cggtccctcg cagcgcgcca gtgccgtcgc gcacctgcag tgcgccgagg gggaagttgg
     2581 cgaaggacga atgatagttc tgaagcgcca gaccgagctg cttcaggttg ttggtacact
     2641 ggattcgcct cgcggcctcc cgcgccgact gcactgcggg gagcaggagt gcaatcagca
     2701 ctgcgatgat cgcgatcaca accagtagct caatcagcgt gaagcctcct ctcggttggg
     2761 ggcgcgccaa atcgtatagt cgtctcacgg gatttccctt cagaatatgt gtggggtatg
     2821 gtcggaagga cggtccgtcg ccggggatgc gtggaccgtg ctaagggggg ttggattgag
     2881 ctcgccggcc cgggtgggcg cctccgtccc gacgttccta ttccggtcgc ggacgggccg
     2941 aggggcctga tcagtgaatt tccctccatg cgaacacgcg agcttatgct atcttaaggc
     3001 agcgtttgta gttttcttgt ggcgacgatg aagatcgcgc gaaacttggc gtcgtggagg
     3061 tttttcagac gcggtctgaa aaactgaggg taaaaggggg cgctgccgtg cctcaggcag
     3121 acgaggcggg ttcgcacgag ggctgggact cgcgccggct cgataccagg cacgggaagt
     3181 cgtgggccga tcgccgttgg tgccgagatc ggacgcctgg cacctcgcgc ccctgccagc
     3241 cagttcacgg ggcggtcttg cgtcgtgcgg agggatgcga cgcgaaccgg acatccgaca
     3301 ggagttccct gcgagcggtc gttcgcaagt tttacgccag cggtgaacac atataacagc
     3361 catatcgtat agatagacgt gctgaacaag gtaagcgtga tgccagccag aggagcgcag
     3421 tcatgctaac cgtgaccaac ctttgctaca tcgcgacagt cgtcaccatc ggcatcctgc
     3481 ttggcgtcgt gatcgggcgg gcgaaagccg atgcaggtgt gaagtgaatc cggctaccgc
     3541 cggccgcggc ctcttgcatg aggggcttgc ggctggtctt gtttcccggc ttcgcctatg
     3601 gcttcatgtt cgagggccga ttgccgtctc ctgcggaggc gatcggggca gcattgcgat
     3661 tcgtcagtgt ttgctcggcc atcgcggcct tca
//
LOCUS       DATAQL010000917         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1315099, whole genome shotgun sequence.
ACCESSION   DATAQL010000917 DATAQL010000000
VERSION     DATAQL010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315099"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHS97_15930"
     CDS             <1..323
                     /locus_tag="VHS97_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3449745.1"
                     /translation="VQSVAVAPDGKTLAGGSGPWRDFPNQTWRDFMDPKQRASGEIRL
                     WDTATGKLLWMVDGELGSVRCLAFAPNGKTLSYCDDRVVGVVDVATGKTVRILVRSTL
                     KPLQ"
     gene            complement(403..921)
                     /locus_tag="VHS97_15935"
     CDS             complement(403..921)
                     /locus_tag="VHS97_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449746.1"
                     /translation="MSQHADLLAQAVSLAKMDVKKPKQANLRRAISSAYYAVFHLLVD
                     EACRAQIGGLHRQAPFRHVLGRAFAHGVMKEACKSFAGGALKKGVAKGLPVGFAIPSE
                     IRELASAFVDLQDHRHLAEYDLTERFKRSDVLLLIAQVRRHIESFKNLAPSNEKQFFL
                     ACLWAWKELANR"
     gene            complement(918..1226)
                     /locus_tag="VHS97_15940"
     CDS             complement(918..1226)
                     /locus_tag="VHS97_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449747.1"
                     /translation="MPVDQKIRNAVKLSKLKAKLPENPRVIDIQVEDYVDTDGEDALR
                     VLVILDESVDVENIRGEDVSALKSAIRNAVRGQGVELWTYIRFAKQSELDEGDEEDEE
                     "
     gene            1525..>3270
                     /locus_tag="VHS97_15945"
     CDS             1525..>3270
                     /locus_tag="VHS97_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX3449748.1"
                     /translation="MRWMTPGIFGCGLTWLLLLAGTKSLSAAEPARADSRKPQIPELK
                     IETYKLPNGLTVILHEDHKTTLVAVNLLYKVGSKDEKPGRTGFAHLFEHMMFQGSKNH
                     DTDYFLPLEKLGAQLNGETEEDQTDYYETVPSNALELALWLEADRMGFLLPAMTQAKL
                     DNQRDVVKNERRQSMDNVPYGQAEEALLAALYPSEHPYHHSVIGSMADLSAAQLADVA
                     AFFRTYYVPNNAILCVAGDFQPAQTKEWIEKYFGPLPRGPEVKRPTPSVPSLSAEKHV
                     KLTDAVSLPRAQLIWPTVPAGHPDEPALDVLAAILGGLSKENRLFRALMYERQLAAQV
                     NASHPTQLLTGKFEVELYARPGESLENLVKMADAEIERLKKDGPTPLEVHKAQNEEES
                     RLIMGLQSVTRKASVLNRFMAVHGNPLAYRTELEKVFAVRPEDVTRVARAYLRPARIK
                     LDIVPGAPASLPAEAAVDRAKEAPLVSPKLAEVNDTFDRSTMPRLGPIPRFVPPSFER
                     RSLSNGLELLIVERHELPILTLDLIVKSGETSTPKGKEGLGSIAASLLDEGTQTRNAL
                     EVAGELAEIGATLAAA"
BASE COUNT          659 a          997 c          954 g          660 t
ORIGIN      
        1 gggttcagtc cgtcgctgtt gctcccgatg gcaagaccct tgctggtggc agcggaccct
       61 ggcgcgattt cccgaatcag acctggcgcg atttcatgga tccgaagcag cgtgccagcg
      121 gcgagatcag gctttgggac accgcgacgg gcaagctcct ctggatggtc gatggagagc
      181 tcggttcagt caggtgcttg gcgttcgcac ccaacggtaa gactctttca tactgcgatg
      241 atcgcgtcgt cggtgtcgtc gatgtcgcca ccggcaaaac cgttcggatc ctggtcaggt
      301 cgactctaaa gcccctccaa taaagcaaag gagtgcaatt aatcgcgacg acttgtgccg
      361 ggccgttgtc gggacatgtg ggacgatcaa agaccgagag tcttaacgat tcgccagctc
      421 tttccaggcc cagaggcaag ccaggaagaa ctgcttctcg ttggacggag ccaggttctt
      481 gaagctctca atgtgtcgcc gcacctgtgc gattagcaga aggacgtcgg agcgtttgaa
      541 gcgctccgtc aggtcatatt cggcgaggtg ccgatgatct tggagatcga cgaatgcact
      601 cgcaagctca cggatttccg agggaatagc aaagcccacc ggcagcccct tggcgacgcc
      661 cttcttgagc gcacccccag caaaggattt gcatgcttcc ttcatcactc cgtgcgcgaa
      721 cgctcttccc agtacatgac ggaacggtgc ctggcggtgc agtcctccaa tttgggcgcg
      781 gcaagcttcg tcgaccagga ggtgaaagac cgcataataa gcggacgaaa ttgcccggcg
      841 gagatttgcc tgcttcggtt tcttgacatc cattttcgcg aggctgacag cttgcgccag
      901 caggtctgcg tgctggctca ttcctcgtct tcctcgtcac cttcgtcgag ctcgctttgc
      961 ttggcaaacc gtatgtaggt ccacaattcc acgccctgtc cgcgaaccgc gttccgaatc
     1021 gcggatttca gggcgcttac gtcctctccc cggatgttct cgacatcgac gctttcatcg
     1081 aggatgacca acacgcgcaa ggcatcttca ccgtccgtat ccacataatc ctcgacttga
     1141 atgtcgatga cacgaggatt ctccggaagc ttcgctttca gcttgctgag cttcacggca
     1201 tttcggatct tttgatcgac cggcatgacc atgctccctt cagtgatgcg gggcctgcct
     1261 acattggccg gggctggatc ggaaagcccc gcgacatggt cattctccag ctcccggccc
     1321 aggagttcaa tctcagtggt gggtcgccag gctccctgga caatcgccga gcacgcctga
     1381 gccgggtcag ccggcttgat ctctagacga tcgacatgca cctaggctag aacagagcca
     1441 ttcgatgtgc gagccggggc cgttctcgga cgttgggcga ttgctaatgc tttttgccgt
     1501 tgtgttcagg gaggttttgc accgatgaga tggatgactc cgggcatctt tggctgtggt
     1561 ctgacttggt tgttgttgct cgccgggaca aagtcgcttt cggcagccga gcccgctcgc
     1621 gccgattcga ggaaaccgca gatccccgag ctcaagatcg agacgtacaa acttcccaat
     1681 ggcctgacgg tgattcttca cgaggaccat aagaccaccc tggtggccgt gaacctcctg
     1741 tataaggttg ggtccaagga cgaaaagccc ggccgtaccg gcttcgccca cttgttcgaa
     1801 cacatgatgt ttcagggctc gaagaatcac gataccgact acttccttcc tctagaaaag
     1861 ttgggtgcgc agctcaacgg cgagaccgag gaggaccaga cggactatta cgagactgtc
     1921 cccagtaacg cactcgagct ggcgctctgg ctcgaagccg atcgaatggg ttttttgctg
     1981 ccggcgatga cgcaggcaaa gctcgacaac cagcgcgacg tggtcaagaa cgagcggcgc
     2041 caatcgatgg acaatgtgcc ctacggccaa gccgaagagg cgttgctcgc ggccctatat
     2101 ccctcggagc atccctatca ccatagcgtg atcggctcga tggccgacct ctcggccgcc
     2161 cagctggccg acgtggccgc atttttccgc acgtattacg ttcccaacaa cgcgatcctc
     2221 tgtgtcgcgg gggactttca gccagcccag accaaagaat ggatcgagaa atacttcggc
     2281 ccgcttccgc gcgggccgga ggtaaagcgc cccacgccaa gtgtccccag tttatcggcc
     2341 gaaaagcacg taaaattaac ggatgcagtt agcttgccgc gtgcccagct catctggccg
     2401 accgtgccgg ccggccatcc cgacgagcca gccctggacg tgctggcggc gatactgggc
     2461 ggtctctcca aagaaaaccg cctgttccgt gccctcatgt atgagcgtca gcttgcggcc
     2521 caggtgaatg cgtcgcaccc gacccagttg ctcaccggaa agttcgaggt cgagctctat
     2581 gcccggccag gcgagagcct cgagaatctg gtcaagatgg ccgacgccga gatcgagcgg
     2641 ctcaagaaag atggtccaac gcccctcgag gtccacaagg cgcagaacga ggaagaaagt
     2701 cgcttgatca tgggcctaca gtcggtgacg cggaaggcca gtgtgttgaa ccgattcatg
     2761 gccgtgcatg ggaatcccct cgcgtaccgg accgagctgg aaaaggtatt cgcggtcagg
     2821 ccggaagacg tcacgcgggt ggcgcgcgcg tacttgcgac cagcgcgcat caagctagac
     2881 atcgtgccgg gcgcgcctgc ttcgctgcct gccgaagcgg cagtcgaccg tgcaaaagag
     2941 gcacctctcg tgagtccaaa actcgcggaa gttaatgaca ctttcgaccg ctcgaccatg
     3001 cccaggcttg ggcccatacc gcggtttgtg ccgccaagtt tcgagcgccg ttcgctttca
     3061 aacggtctcg agcttttgat cgtcgagcgc cacgagctgc caatcctgac cctcgacctg
     3121 atcgtcaagt ccggcgagac ctccacgccc aagggcaagg aaggtttggg atcgatcgcc
     3181 gccagcctgc tcgacgaggg cacccagacc cgcaacgctc tggaggtagc cggcgagctc
     3241 gccgaaatcg gcgccaccct tgccgctgcg
//
LOCUS       DATAQL010000918         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4826707, whole genome shotgun sequence.
ACCESSION   DATAQL010000918 DATAQL010000000
VERSION     DATAQL010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4826707"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1284)
                     /locus_tag="VHS97_15950"
     CDS             complement(19..1284)
                     /locus_tag="VHS97_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX3449749.1"
                     /translation="MPQDELATLLPPSSHPELVSASPGIRFIVLLGLLDAFGPLGIDM
                     YLPAFPHIEQGLRAQGGAMQLTLSLFLAGLAIGQLICGPISDRVGRRLPLLYGSVAFA
                     VASVACAYARSIEALIAARFIMGLAGSTGMVIARAVVRDTFEESDSSRIYSMLMLVIG
                     IAPIISPSVGGWLLQLGGWGSIFWALAGFACVCGAAVAIDLPETHPVERRNRDSITTV
                     FRRYGALLVDLRYMGYAAPVSLALGLIFAYVASSPSLFMQYYKLSPTAFSLLFASNAV
                     GLIGAAQVNRWLTRHYDTHAILRAASIVNALAALMLPVLAWTGIGGFPAIFADLFLCL
                     MTVGLILPNATAAVMAPYPHQAGVASALLGMLQFSVGAATGAVVGLFHDGTARPMALT
                     MASCAAILLVIILASERMNKIHSMSVAQS"
BASE COUNT          422 a          520 c          601 g          320 t
ORIGIN      
        1 cgcggccctg gtttgccgtc acgattgcgc gacactcatc gagtgaattt tgttcatccg
       61 ctcgcttgct agtattatca ctaataaaat tgcggcgcaa ctcgccattg taagggccat
      121 ggggcgagca gtcccgtcgt ggaagaggcc gacgaccgct ccggtcgcgg cgccgacgga
      181 aaactgaagc atcccgagca gagccgatgc gactcccgcc tggtgcggat acggggccat
      241 caccgcggcg gtcgcgttgg gtagtatgag tccgaccgtc atcaagcaca ggaacaggtc
      301 agcaaagatg gcggggaaac cgccgattcc ggtccaggcg aggacgggaa gcattaaggc
      361 cgccagcgca ttgacgatcg aggcggcccg gaggatcgcg tgggtatcat agtgccgggt
      421 cagccagcgg ttgacctggg cggcaccgat caagccgacg gcgttgctgg cgaaaagcaa
      481 actgaaggcc gttggtgaga gtttgtaata ctgcatgaac agggacgggg acgacgctac
      541 ataagcaaag ataagtccca gcgcgaggct caccggagcg gcgtagccca tgtagcggag
      601 gtcgaccagg agcgcaccgt accggcgaaa gacggtcgtt atggaatctc gattacgacg
      661 ctcgactgga tgcgtttcgg gaagatcgat ggcgacggcc gcgccgcaca cgcaagcaaa
      721 gcctgcgagc gcccagaaaa tcgatcccca gccgcccaat tgcaagagcc atccgccgac
      781 ggaaggcgaa atgatgggtg cgatgccgat cacgagcatc agcatggaat agatgcgcga
      841 tgagtcggac tcttcgaagg tgtcgcggac cactgccctc gcgatgacca tgccggtcga
      901 gcccgccagt cccatgataa accgagcggc gatcagtgcc tcgatcgagc gggcgtaagc
      961 gcatgccacg gaagcgacgg caaaggcgac cgatccgtaa agaagcggca accgccggcc
     1021 aacgcggtcg gaaatcgggc cgcagatgag ctgaccgatg gccaggccgg ccagaaagag
     1081 cgacagcgtc agttgcatcg cgcctccctg tgcccgcaaa ccttgctcga tatgcgggaa
     1141 cgccggcagg tacatgtcga tgccgagcgg cccgaacgca tcgagcagac cgaggagaac
     1201 aataaatcga attcccgggc tcgcggagac caactctggg tgcgaggagg gaggaaggag
     1261 cgtcgccagt tcgtcttggg gcattatttc gtctcggatg tctcgattag caggcgcccg
     1321 agggagagaa tcggtgagcg cgaaccgtgg ggcgtacatg ggaagcagaa taaataagat
     1381 tacccgataa cacgagcggg cccaagagat aatttcaggc ggcgacgact ccgacggtta
     1441 attcaggcat gcaattgttt tcaatcgctg cctggttctt tgtcgggttc gagcgacagg
     1501 gtattggatc ggctcgcggc ggcatcggcg tgatgcatgc cgacgactct ctttccttct
     1561 tttcacacaa agtccgtaga aggtcactac atcggggtcg gcgtctacag gctttccggg
     1621 tgtggcgaga cagtagactt cgtaaatcac gtgtgacgaa tctgcgttca tgatctcgga
     1681 catttcaccg gccggaatca cgcgggattc tgggctcagg taatagcggg ctgcagccag
     1741 ttgctcgcga ggaaaccgag ggtcgctggg atgaaccacg atcgcccgag acctggaagg
     1801 cgagtcacct gccccgtctt cactctcgtg acctttcagc cagatagcca ggccgtgcca
     1861 gtc
//
LOCUS       DATAQL010000919         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5132156, whole genome shotgun sequence.
ACCESSION   DATAQL010000919 DATAQL010000000
VERSION     DATAQL010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5132156"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VHS97_15955"
     CDS             complement(<1..248)
                     /locus_tag="VHS97_15955"
                     /inference="COORDINATES: protein motif:HMM:NF015180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HEX3449750.1"
                     /translation="MTDLPRGPLGILDMFGTVASLLCAVHCAVTPMAMALLPLVGLEF
                     LGAEATECTLVGLSIVVGSVSLVLGYGRHRSVRTLGIFS"
     gene            complement(282..509)
                     /locus_tag="VHS97_15960"
     CDS             complement(282..509)
                     /locus_tag="VHS97_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449751.1"
                     /translation="MTEIREFVLCRDNGTCCFGGQPKLADMIQVSLKEPLTLDYHTGL
                     RHVAGTFRVEPKTATGGLGSVLYRLEADYAR"
     gene            complement(552..947)
                     /locus_tag="VHS97_15965"
     CDS             complement(552..947)
                     /locus_tag="VHS97_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX3449752.1"
                     /translation="MTAQQTATDLYQELDYRPTSPMALLSVVVGLLALVSACLVYDLG
                     STTFVLASAIGLVTAYRGLSAVRRYEMRGRGAAKAGLGLSALALVAGFGINGLQTAFE
                     VPTGCLPISYETLQPKPGESVPVSAREFD"
     gene            complement(1030..2388)
                     /locus_tag="VHS97_15970"
     CDS             complement(1030..2388)
                     /locus_tag="VHS97_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3449753.1"
                     /translation="MSLIAIAWRSLTQRALASALTGFSMALGVALVVTVMVIHHVIAD
                     SFQRNSRVGYDVIVGAKGGKLQLVLNTIYHLSTPVENIPYSVLHDLTHGQYKPYVAKA
                     LPYCLGDNYEGHRVVATVAEIFQDSEYASGRKYAFKDGRNFRPTGFFEGVVGALAAER
                     TGLKIGDKFRPAHGVNDDGQGHEHNAFTVVGILEPTGTPNDRAIFINLEGFYLLEDHA
                     KPEALVRRPGQDALVHDTGGRDEHGESHDVTHEGNEGEGHERHDLTPLPEERRELTAV
                     LVRTSSPAASIRLPNLINEGPVAQAVLPVREIRALFDVIVGPIETVLLVLTALIVVVS
                     AVGILVSIYTSMNERRREIAVMRALGARRSTVRTIVLLESILLSLTGGLAGWMLGRAL
                     IAALSPAIAARTGVVIGLAPLVAAELALVPALTLLAALAGYLPAMTAYRTDVAKALAS
                     AS"
     gene            complement(2385..>2901)
                     /locus_tag="VHS97_15975"
     CDS             complement(2385..>2901)
                     /locus_tag="VHS97_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3449754.1"
                     /translation="AGISTPDSGEIRVDGRDITQLDEAGRDRFRAETIGYVFQSFNLL
                     SGFTALENVLLGLAFSQGRHDAARARDLLGRVGLSHRHRHKPAELSVGEQQRVAVARA
                     LANRPRLLLADEPTASVDSRHQQTVIDLIRQTCEEEGVALLLVTHAPDVAARFERAVH
                     LENLNKVAATL"
BASE COUNT          571 a          922 c          887 g          521 t
ORIGIN      
        1 gagaagatgc ccaacgtgcg aaccgagcgg tgccgcccgt agcccaggac taggctcaca
       61 cttcctacga cgatggagag ccccaccagc gtacactccg tggcctcggc cccgaggaac
      121 tcaagaccga ccaacggtaa aagagccatg gccatgggcg tcacggcaca atgcacggcg
      181 cagagcaacg aggcgaccgt gccaaacatg tccaaaatcc ccaagggccc tcgcggaaga
      241 tcggtcattt cgccattcct tcgtgattct gtaagaggac cttagcgcgc ataatccgcc
      301 tcgagccggt agagaacgga gcccaggccg cccgttgcgg tcttcggttc gacccggaac
      361 gtcccggcca catgccgcaa tccggtgtga tagtcgagag tgagcggttc cttgaggctt
      421 acttggatca tgtcggcaag tttgggctgg ccgccaaaac agcacgttcc attatcccga
      481 cagaggacga actcgcggat ctccgtcatc tgactgctcg gatacatata gcccttgata
      541 aacaccttct ttcagtcgaa ctccctcgcc gacacgggga ccgactctcc gggcttgggc
      601 tgcaaggttt cgtagctgat tggcaggcag cccgtaggta cttcgaacgc agtctgtaag
      661 ccgttgatcc caaaaccggc aaccagagcc aaggcggata ggcctagacc ggccttggcg
      721 gcgccccggc cccgcatctc gtagcggcgc acggcggaga ggccgcggta cgcagtcacc
      781 aggccgattg cggaggcgag cacgaatgtc gtcgacccca ggtcgtaaac cagacatgcc
      841 gagaccagag caaggagccc cacgaccacg ctcaagagcg ccatcgggct cgtcggccgg
      901 taatcgagtt cctggtaaag gtcggttgca gtttgttgag cggtcattct gttctccatg
      961 actttcgttc ctcagtcccc tctctttcgt attgacttcg tctgcgatct gaatgtcacc
     1021 gccgcgtggt caagatgccg aagccagcgc cttggcgacg tcggtccggt aggctgtcat
     1081 cgccggcagg tagccggcca gggcggccag cagggtcaag gccggcacga gcgccaactc
     1141 ggcggcgacg agcggtgcga gcccgatcac aactcccgtc cgggcggcaa tcgccgggct
     1201 cagtgcggcg atcaaggccc gtccgagcat ccaacccgcc agtccccctg tcagcgaaag
     1261 caagatcgac tcgaggagca caatcgttcg caccgtcgac cgccgcgcgc ccaaggctcg
     1321 catcacagcg atctcccgcc tccgctcatt catcgaagtg tagatgctca cgaggatgcc
     1381 caccgcggaa acaacgacga tcaatgccgt gagcacgagc aagacggtct cgatcggacc
     1441 gacaatcaca tcgaagagcg cacggatctc cctgaccggg aggacggcct gcgccaccgg
     1501 cccctcattg atcagattcg gcaggcgaat cgaggcggcg ggcgacgacg tgcggaccag
     1561 aaccgccgtc agctcgcgcc gctcctcggg aagcggtgta aggtcgtgac gctcatgccc
     1621 ttcaccttcg tttccctcgt gtgtaacgtc atgcgactct ccgtgctcat cgcggccgcc
     1681 tgtatcgtgt acgagagcgt cctgcccggg tcggcgcaca agggcttcag gcttggcgtg
     1741 gtcctcaagc aggtaaaacc cctccagatt gatgaagatt gcgcggtcgt tcggcgtccc
     1801 ggtgggttcg agaatgccga cgaccgtgaa ggcattgtgc tcgtgcccct gtccgtcgtc
     1861 gttgacgcca tgggccggcc gaaacttgtc gccgatcttc agccccgttc gctccgcggc
     1921 cagggccccc acgaccccct cgaagaagcc ggtgggacga aaattccggc cgtccttgaa
     1981 cgcatatttc cgccctgatg cgtactcaga atcctggaaa atctcggcca cggtggcgac
     2041 cactcggtga ccttcgtaat tgtcgcccag gcagtacgga agcgccttgg ccacgtacgg
     2101 cttgtactgt ccgtgggtaa gatcgtgcag aacggagtag gggatattct cgacaggtgt
     2161 gctcaggtgg taaatggtgt tcagcacaag ctggagcttc cctcccttgg ccccgacaat
     2221 cacgtcgtag ccgaccctgg agttgcgctg gaatgagtcg gcgatgacgt ggtgtatcac
     2281 cattactgtc acgaccagcg cgacccccag agccatcgag aatccagtga gagcggacgc
     2341 gagcgcgcgt tgcgtcaggc tccgccaggc gattgcaatg aggctcatag agtggctgcc
     2401 actttgttga ggttctccag gtgtacggcc cgttcaaacc gggccgcgac gtccggggca
     2461 tgcgtgacca agaggagagc aacgccctcc tcctcgcacg tctggcggat gagatcgatc
     2521 acagtttgct ggtggcggga gtcgacactg gcagtaggct cgtcagcgag gagaagtcgt
     2581 ggccggttcg ccagggcccg tgccaccgcc acccgctgct gctcgccgac tgagagctcg
     2641 gcgggcttat gccggtgacg gtgcgagaga ccgactcgtc ccagtaggtc tcgggctcgg
     2701 gcggcgtcat gtcgaccttg actgaacgcc aggccgagca ggacattttc gagcgcggtg
     2761 aacccagata agagattgaa tgactggaaa acgtagccga tagtctccgc gcggaacctg
     2821 tcacgtcccg cctcgtcaag ctgtgtaata tcgcggccgt cgacacggat ctcgcccgag
     2881 tcgggcgtac tgatcccggc g
//
LOCUS       DATAQL010000920         6100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_855765, whole genome shotgun sequence.
ACCESSION   DATAQL010000920 DATAQL010000000
VERSION     DATAQL010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855765"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="VHS97_15980"
     CDS             <1..170
                     /locus_tag="VHS97_15980"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449755.1"
                     /translation="ALNPHIGYDNAAKIAKNAHKKGITLRESAIELKLLSPDQFDAWV
                     RPEDMVGQQNK"
     gene            395..2845
                     /locus_tag="VHS97_15985"
     CDS             395..2845
                     /locus_tag="VHS97_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3449756.1"
                     /translation="MIRHGTFALVILATVFLTNPVNGQTRYRQPPADILAILDAAPPP
                     RAIESPTRDALLLVEMKPYPSIEVVAQPVLRLAGLRINPKAGCLQRTITHTGLSVQPL
                     DGSPRRRIEIPEGASIQGPSWSHDGKRIAFARDIEDGVELWIADTTTGRAKPIVGVRL
                     NDVLAGEIVWQSDNRHLFAVLVPEGRGQAPAGPRAPAGPNVQVTSGRHSQMATFQDLL
                     ASEHDEDLFQHFATGQLARIDTETGEIERVGPAALITAVSASPDEKYLLVGTVRRPFS
                     YRVPFLYFTRKTEVWSATGHPVATVADLPISDDVPRQGVPTGPRRVRWQPLHDARLVW
                     SEALDGGDPRTKVPHRDKVMALAAPFTGRAEELMKVQHRLAGFEWLPDRDHALITELD
                     RDRRWRTTALVDLAHPVESRKVLFDLSVNDAYQDPGDPLTVSRPDGTRTILKDRDSIY
                     LTGDGASPDGSRPFLDRLDLKTGQKARLFNCRELVFENALGFVGDSRSTILVRHQSKT
                     EPVNYFTVDLTSGKRTKLTHFLDPAPQLTGLSKELIKYKRDDGVPLSGTLYLPPDYKE
                     GTRLPLIVWAYPLEYSDPGTAGQVRTSPYMFTRMAGPSQLFFVTRGYAVLDNATMPVV
                     GDPETMNDTYVEQISAAARAAIETLDKRGVIDPKRVGVGGHSYGAFMTANLLAHTDLF
                     AAGIARSGAYNRSLTPFGFQSERRSYWEAVDLYTRMSPFTYAHKINEPILLIHGEADN
                     NSGTFPIQSERLFQAINGNGGTARLVMLPFESHGYRARETVLHVLAEMVDWADQYVKN
                     RSLSSGSPAASAARRPAR"
     gene            complement(3106..4527)
                     /locus_tag="VHS97_15990"
     CDS             complement(3106..4527)
                     /locus_tag="VHS97_15990"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX3449757.1"
                     /translation="MGRPEKNESSDPRAGSAAEIAQRIGGDPSPSESSGTFTPAVIED
                     IQIKSELGRYRIRGFGSLRPRTWATLDDLTFIPGTLTRIPLEGYREACLTRTVLGTRF
                     ASKPIELEIPLMVTGMSYGALSKNAKTALARGARAAGTSTTTGDGGMLDVERTESQVL
                     VYEVLPSRYGINIQHLRQADAIELTIGQGAKPGTGGLLLGSKVSDEIARIRDLPSGVD
                     QRSPCRHPDFLGPDDLVIKIEELREATDHRVPIFVKMGASRVFDDVRLAAKAGADVIV
                     VDGMEGGTGASPELLQEHTGIPTLAAVCEARAALEDIGLYGIVQLVIAGGLRHGSDCA
                     KALALGADACYLGTALLIALNCNKPIYVEDYHKIGAAPYSCHHCHTGRCPVGITTQDP
                     ELTARLDIDQAALRVANFFHALTSEIQILARACGKSDVHHLEPEDLRALTLEASLITG
                     LPLAGTNRVFGGGPGWKDQNAGH"
     gene            complement(4527..5429)
                     /locus_tag="VHS97_15995"
     CDS             complement(4527..5429)
                     /locus_tag="VHS97_15995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449758.1"
                     /translation="MSAAIIFDRFINWPSKTALAVAVASAIVLIIAGLLFAVKKWKWF
                     GYSLGLLGFLIIMGLLIIIHEQTRIEKLNENITVTRYRYSERTRLWTIVAMSGLPAAA
                     AAVIGVGFITTRHRLRGEVPAHLKLGRRHLAQKHYEAALREYNWAIQAAPDLAEAYCK
                     RGSIYQAIGKPEKALSDFDRAIQFDPRLAAAYLERGKMRTERGEHDAALADFGQLMLI
                     RAHDPDSYLHRGICLAKKGFIDDALADFHRVLKLTNHSDFAEPAKDFIRQLLDQPGKS
                     PPPNANGTPAIATAATHQAQDHAI"
     gene            complement(5701..>6100)
                     /locus_tag="VHS97_16000"
     CDS             complement(5701..>6100)
                     /locus_tag="VHS97_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEX3449759.1"
                     /translation="AASIRAGTVVVRGDVSTRAGIAMKGGTVIVAGDAGPMAGFMMQK
                     GVLIVCGDAADGVAESMYAGTVFLGGRHGELGADAVFEDLTAGDREFLGAALERHSVA
                     APAAGFRKLVAGKRLWNFQNSERELWKSAL"
BASE COUNT         1161 a         1909 c         1855 g         1175 t
ORIGIN      
        1 ccgcactcaa cccccatatc ggttacgaca acgcggccaa gatcgccaag aacgctcaca
       61 agaaggggat cacacttcgt gagtcggcca tcgagctcaa actgctcagc cccgaccagt
      121 tcgacgcatg ggttcgtccc gaagatatgg ttgggcagca gaacaagtga gatggctcgg
      181 cgcaacggcc cggatgggga ggaaacggcc gggcggcgtg gcacggcaac cctttttccg
      241 atgtgcaacc cgttcggatt tgcgtggagc gatactcatg aggaccctga ttgcttcaag
      301 ccgaagcgcc gagtaacatg cccgggctca atcccacgac cgatcccgcg gtctcttacg
      361 cgtcgtccca ctcacgatcg aggaagtctc cgccatgatc cgccacggca cgttcgctct
      421 cgtcatcctc gcgaccgttt ttttgaccaa ccccgtgaat gggcagacac gctaccggca
      481 accaccggcc gacatcctcg ccatccttga tgcggctcct cctccgaggg cgatcgagag
      541 ccccacgcgc gatgctctgt tgctggtcga gatgaagcct tatccatcga tcgaggtcgt
      601 cgcccaaccc gtgctgcgtc ttgccgggct gcggatcaac cccaaggccg gctgcttgca
      661 acgcaccatt acccacaccg ggctctccgt ccagccgctc gacggttctc cccggcgccg
      721 gatcgagata cctgagggag cgtcgatcca ggggccgagt tggtctcacg acggcaagag
      781 gatcgcattc gcacgcgata tcgaagacgg ggtagagctc tggatcgcag acacgacgac
      841 cggccgggcg aagccgatcg taggagtacg gctcaacgac gtgcttgccg gtgaaatcgt
      901 ctggcagagc gataatcggc acctgtttgc tgtgctcgtg cccgaggggc gagggcaagc
      961 gcccgcaggg ccgcgggcgc ctgcgggtcc caacgtgcaa gtgacgtcgg gccgccacag
     1021 tcagatggct acctttcaag atttgctcgc ttccgaacat gatgaagact tattccagca
     1081 cttcgctacc ggccagctcg cccggatcga taccgagacc ggcgaaatcg agcgtgtcgg
     1141 accggccgca ctgatcaccg cggtcagcgc gtctcccgac gagaagtatc tcctggtcgg
     1201 cacggttcgc cgcccgtttt cgtaccgggt gccgtttttg tacttcacgc gcaaaaccga
     1261 ggtatggagc gccacggggc atccggtcgc gacggtcgcc gacctgccga tcagcgacga
     1321 cgtcccccgc caaggcgtgc caactggccc gcgacgcgtg cggtggcagc cgctccacga
     1381 cgctcgtctg gtctggtccg aggctctcga tggcggcgat ccgcgcacca aggtgcccca
     1441 tcgcgataag gtgatggcat tggccgctcc gttcaccggc agggccgaag agctgatgaa
     1501 ggtgcagcat cgtctggcgg gctttgaatg gctgccagac cgagatcacg ctctaattac
     1561 cgagcttgac cgcgatcgcc gctggcgcac aaccgcgctt gtggacctgg cccatccggt
     1621 cgaatcgcgc aaagtcctct tcgacttgag cgtcaatgat gcataccaag atccaggcga
     1681 tcccctgacc gtgtcccgtc ccgacggaac tcggacaatt ctaaaagatc gcgattcgat
     1741 ctatctcacc ggcgacgggg cctcgccgga cggctcgcgg ccgtttctcg atcggctcga
     1801 tcttaagacc gggcagaaag ccaggctgtt caactgccgc gagcttgtat tcgagaacgc
     1861 tctcggtttt gtaggcgact cgcgttcgac gatcctcgtc cgtcaccagt ctaaaactga
     1921 gcccgtcaat tacttcacag tcgatctcac gtcgggaaaa cggaccaaac taacgcactt
     1981 tcttgaccct gcaccccagc tcaccgggct ctcgaaagag ctgatcaagt ataagcgcga
     2041 cgacggcgtc ccgctctcgg gcacgctcta cctgcccccc gattacaagg aagggacgcg
     2101 actccccttg atcgtctggg cgtatcccct tgagtacagc gatccgggca cggctggcca
     2161 ggtacggacc tcaccttaca tgttcacgcg aatggcaggg ccttcccagt tgtttttcgt
     2221 cacgcgcggc tacgctgtac tggataatgc gacgatgccg gttgtcggtg acccggagac
     2281 gatgaatgac acttatgtcg agcagatatc ggccgcggct cgcgcggcca tcgagacgct
     2341 cgacaagaga ggcgtgatcg acccaaaacg ggtcggcgtc ggcggtcaca gctacggtgc
     2401 gttcatgacg gccaacctgc tggcgcatac cgatctcttt gccgccggca tcgcgcgcag
     2461 tggcgcgtac aaccgttcgc tcacaccgtt tggattccag tcggaaaggc gtagctactg
     2521 ggaagcagtc gacctttaca cgcggatgtc cccctttact tacgctcaca agatcaacga
     2581 gccaattctg ttgattcatg gcgaggccga caacaactcg ggaacatttc ccatccagtc
     2641 ggagcggctt tttcaagcga tcaatggcaa tggcggcacc gcgcggttgg tgatgctgcc
     2701 tttcgagagt cacggctacc gtgctcgaga gacggtcttg cacgtcctcg ccgaaatggt
     2761 cgattgggcc gatcaatacg tcaagaatcg ctcgctctcg agtggctcgc cggcggcttc
     2821 ggcggcgcgg aggccggcgc gttaaggcca agaacgttag attcagtatg ccttaaggcc
     2881 gcgacagctt ttctgtcacc ctcccccctt gtggtggagg gttggggtgg ggggaggcag
     2941 gcaccaataa tcggctcggt tgcgttccac gaggcgaacg ccccacaccc tggccctccc
     3001 ccaccagggg ggaggggatt ataaactggc atgcacaagt agtgtcctgc taaatcaacg
     3061 acattgacat cggcgtggcg aaaaaccccc cgacaacaca actggtcagt ggccggcgtt
     3121 ctggtctttc caccccggtc cgccgccgaa gacgcgattc gtgccggcga gtggaaggcc
     3181 ggtgatcagc gatgcttcga gggtcagcgc acgcaggtct tccggctcga ggtgatgtac
     3241 gtcgctcttg ccgcaggcgc gggccaggat ctggatctcg gaagtcaggg cgtggaaaaa
     3301 gttggcgact cgaagggcgg cctggtcgat gtcgagtcgg gcggtcagtt cggggtcttg
     3361 agtggtgatc ccgaccgggc agcggccggt gtggcagtga tggcacgagt acggggcggc
     3421 gccgatcttg tgatagtctt cgacatagat cggcttgttg caattgagcg cgatcaggag
     3481 ggccgttccc agatagcagg cgtcagcacc tagcgccagt gccttggcac aatcggaacc
     3541 gtgacggaga ccgccggcga tcacgagctg cacaatgccg taaagcccaa tgtcttcgag
     3601 tgccgcccga gcttcgcaaa cggcggccag ggtcgggata ccggtgtgtt cctggagtaa
     3661 ctcgggagac gctccggttc cgccttccat gccgtcgacg acaatcacat ccgctcccgc
     3721 cttggcggcg agccgaacgt cgtcgaaaac ccgcgaggcc cccatcttga cgaatatggg
     3781 cacgcggtga tcggtcgctt cgcgtagctc ttcgatcttg attacgaggt cgtcgggtcc
     3841 gaggaagtcg ggatgccggc acggagagcg ttgatcgacg ccggaaggga ggtcgcgaat
     3901 gcgggcgatc tcgtcggaga cctttgagcc caagagcagt cccccggtcc ctggcttcgc
     3961 tccctggcca atggtcagct cgatggcgtc ggcctgccgg aggtgctgga tgttgatgcc
     4021 gtaccggctg ggcaacactt cataaaccaa tacctgcgac tcggtgcgtt cgacgtcgag
     4081 catgccgcca tcgccggtcg tggtcgacgt gcctgcagca cgcgctccgc gcgcaagtgc
     4141 cgtcttggcg ttcttggaga gagctccata gctcatcccg gtcaccatga gggggatctc
     4201 gagctcgatg ggtttggacg cgaatcgagt tccgagaaca gtccgcgtca ggcaagcctc
     4261 gcggtaccct tccagcggga tccgggtgag cgtgccaggg atgaacgtga ggtcgtccaa
     4321 tgtggcccag gttcgaggcc gaagcgatcc gaagcctcgg atgcgatacc ggcccagctc
     4381 ggacttgatc tggatgtctt cgatgacggc gggcgtaaag gtccccgacg attcgcttgg
     4441 cgaggggtca ccaccgatcc gctgggctat ttcggccgcc gatcccgctc tcggatcgga
     4501 cgattcgttt ttttccggtc ggcccatcaa atggcatgat cctgggcttg gtgcgtagct
     4561 gctgtcgcta tggccggcgt gccgttggcg ttcggcggcg gcgacttgcc gggttggtcg
     4621 agaagctgcc gaatgaaatc cttggctggc tcggcgaaat cggaatgatt ggtcaacttg
     4681 agcacacggt ggaaatcggc cagtgcatca tcgatgaacc cctttttcgc caggcagatt
     4741 ccgcggtgca agtacgagtc ggggtcgtgg gcacgaatta gcatgagctg accgaaatcg
     4801 gcgagggccg cgtcatgttc gcctcgctcg gtccgcatct tgccgcgttc caagtaagcc
     4861 gcagcgagcc gtgggtcgaa ttggattgcc cggtcaaaat cgctgagagc cttttccggc
     4921 ttaccaatcg cctgataaat cgagccacgc ttacagtagg cttcagccag gtcaggcgcg
     4981 gcttggatgg cccagttgta ctcgcggagg gcggcctcgt aatgtttctg ggccagatgc
     5041 ctgcgcccca gcttgagatg ggcgggaacc tcgccgcgga gtcggtggcg cgtcgtgata
     5101 aagcccacgc caatgaccgc cgcggcagcg gccggtagcc cgctcatggc cacgatggtc
     5161 cacagacgcg tccgttcgga gtagcggtac ctcgtgaccg tgatattttc gttcaacttc
     5221 tcgatgcgcg tttgctcgtg gatgatgatc agcaaaccca tgatgatcaa aaaaccgaga
     5281 aggcccagag agtaaccgaa ccacttccat ttcttgaccg caaacagtag gcccgcaatg
     5341 atcaaaacaa tcgcgctcgc aaccgccaca gcgagcgccg tcttgctcgg ccaattgatg
     5401 aaacgatcga atattatagc ggcactcatc ccgcgaaacc cttcgcccat acggtccgcc
     5461 agttgtcctg gattcatatt tcgaccgcac gcgtgccgag aacgatcccc cgtgccccaa
     5521 ccgccgttag gtttttgccg acgtccccgg gttgtaaaca tgtattgcga agaggaggag
     5581 cgaaccgacg gtacgacgat cctcgtcgcg ccgccatcac tctacgaggg ccgcgggccg
     5641 ctgtcaatca actgtcaggg cttgtcagct actttttcga caacgggcct gagcggccga
     5701 tcacagagct gatttccaca attctctttc actgttctgg aaattccaga gccgctttcc
     5761 cgccaccagc ttgcgaaatc ctgcggctgg ggcggcgaca ctgtggcgct cgagtgcggc
     5821 tcccagaaac tcgcggtcgc cggcggtgag atcctcgaaa acggcgtctg cacccagttc
     5881 gccatggcgt ccgcccagaa ataccgtccc ggcatacatg ctctcggcga cgccgtcggc
     5941 cgcatcgccg cacacaatga gaactccctt ctgcatcatg aagccggcca tgggtccggc
     6001 gtcgccggca acgatcaccg tccccccttt cattgcgatg ccggcccgcg ttgacacgtc
     6061 gcctcggacg accaccgttc ccgcgcggat cgacgcggcc
//
LOCUS       DATAQL010000921         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1775135, whole genome shotgun sequence.
ACCESSION   DATAQL010000921 DATAQL010000000
VERSION     DATAQL010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775135"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2272)
                     /locus_tag="VHS97_16005"
     CDS             complement(<1..>2272)
                     /locus_tag="VHS97_16005"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX3449760.1"
                     /translation="FEIGLTTSRDVYLDGESFQLQVVTTDARGEPAGHKLSAALLKLV
                     ESRGNITEREIQRKPLETDHKTGRGSLSFRVDDTQGGRYVVRVAGTDRFDNPIVADRF
                     LTISGKKDETKLRLLADRQRYKVGEEARINLHSRDRAGTALVTWEADRILSYKIITLK
                     EGDNAIAWEIAGVQFPNFTLTATRMWHNESNQAKLDIQVERDLRVTVVPTKPVVGPGE
                     QVELEVATVDQLGRPVSAELSIAMVDQSLLRLFDDRLPAIGPFFYNQTRTGAFATEAT
                     NTFCYAPATISVSQAVVDEADRLAAVARNSAELGRVMEEAKSTVLRRESAPASVLAGA
                     TEAREWAAQLPGAPLAKSKNGRADRQRRYGKSVQLFSSEGVEKLGELKDKDLKKHAAA
                     DFLVDNWAEQFGEIQSRERFVETAYWNPKVVTGTDGKARVTFKAPTALSGYRITARGV
                     TGADTLAGQTTAALTVRKNFFVELKLPSALTQGDKPRFVARVHHSDVAGKLALRLAIY
                     AGGRDEIFPGTLDIKQDGIDEVSFEPFLVPEGDSVRLTLTGTIGERTDEVIAEAPIRP
                     WGVRVAASESGTSSESTTVFVGLPAGRSYESSEMQLVLSPTLERMVIELALGQEAYVL
                     GAGSADPRPTGLLSKSPPGQIWPPCNTTSDRAADLLAATAALQYVKTSRATAAPEAAR
                     LTQRIGGLVSSLISAQNEDGGWPWVSGGPLPRSSGKQQLAASERLASAAVVWALASAQ
                     PLGLVTDVKVLDHAGA"
BASE COUNT          399 a          745 c          683 g          445 t
ORIGIN      
        1 taagcccctg cgtgatcgag caccttgaca tcggttacga gccccagagg ctgggcggac
       61 gccagggccc agacgaccgc agccgaggcg agacgctcgc tcgcggcgag ctgctgttta
      121 cctgacgacc ggggcagggg acctccactg acccatggcc acccgccgtc ttcgttttgc
      181 gccgagatca acgaggatac caacccacca atccgctgcg tcagccgcgc cgcctcgggc
      241 gcggcggtcg cgcgggacgt tttcacatat tgcaaggccg ccgtcgcggc taataagtcg
      301 gcggcgcgat ctgaggtcgt gttgcacggc ggccagattt gtccgggcgg gctcttgctc
      361 aagagacccg ttgggcgcgg gtctgccgac cccgccccaa gaacgtaggc ttcctggcca
      421 agggccagct cgatgaccat gcgctcgagc gtcggcgaca agacgagctg catttcggaa
      481 ctctcgtaag atcttcccgc gggcagtccc acgaagacgg tagtgctctc ggagctggtg
      541 cccgattcgg aggcagccac tcgcactccc cagggtcgga tcggcgcttc ggcgatcacc
      601 tcgtcagtcc gctcgccgat tgttcctgtg agcgtcaacc gcaccgaatc cccctccggg
      661 acaagaaacg gctcaaacga tacctcgtcg attccgtctt gcttgatgtc gagtgtcccg
      721 ggaaagatct cgtcgcgccc tccggcgtag atcgctaaac ggagtgccaa cttgccggcg
      781 acgtcggaat gatgcacccg cgcgacaaac cgtggcttgt ccccctgggt gagcgcgctc
      841 ggcagcttga gctcgacaaa aaagttcttc cgaacggtca gagccgctgt cgtctgacct
      901 gccaatgtgt ccgctccggt gacaccccgc gccgtgatgc gatagcccga cagcgccgtc
      961 ggtgccttga aagtgacccg ggccttgcca tccgtgccgg tgacgacctt cgggttccag
     1021 taggccgtct ccacgaatcg ttcgcgggat tgaatctcgc caaattgttc cgcccagtta
     1081 tcgacaagaa agtcggcagc cgcgtgtttt ttcaggtcct tatccttgag ctcgcctagc
     1141 ttttcaacac cttctgagga gaacaattgc acgctcttgc cgtagcgccg ttgccggtca
     1201 gcgcgtccgt tttttgattt tgccagcggc gccccgggaa gctgggccgc ccattcacgc
     1261 gcctccgtcg cgccagcgag taccgacgcc ggagccgact cgcgccgcag aaccgtggac
     1321 ttcgcttctt ccatgacgcg ccccaactcg gcgctgttgc gagccaccgc ggccagccga
     1381 tcggcctcgt cgaccaccgc ctgtgagaca gatatcgtcg cgggagcata acaaaatgtg
     1441 ttggttgcct cggtcgcgaa cgcaccggtc cgggtctggt tgtaaaagaa cggaccgatc
     1501 gccggcagcc tgtcatcaaa gagccgcaag agcgactggt cgaccatcgc gatcgaaagt
     1561 tcggccgaaa ccggccgtcc gagctgatcg actgtggcaa cctcgagctc cacctgttcg
     1621 ccagggccga caaccggctt ggtgggaacg accgtgacac gcaggtcgcg ctccacctga
     1681 atgtccagct tggcctggtt agactcgttg tgccacatcc gcgtcgcagt gagcgtgaaa
     1741 ttggggaact gaacgcccgc gatttcccac gcaatggcgt tatccccctc cttgagagtg
     1801 atgatcttgt agctcaggat ccggtcggcc tcccacgtga ccagagcggt gccagctcgg
     1861 tcgcggctgt ggagattgat cctcgcttcc tcgccaacct tgtaacgttg gcgatcggcc
     1921 agcaacctga gcttggtctc gtctttcttg cctgagattg tgagaaagcg gtccgcgaca
     1981 atcgggttat caaatcggtc ggtgccggcg acgcgcacca cataccgtcc cccctgggtg
     2041 tcgtcgactc ggaaactgag cgatccccgc ccggtcttgt ggtcggtttc gagcggcttg
     2101 cgctgaattt cccgttcggt gatgttccct cgactctcga ccagcttgag cagagcggcc
     2161 gaaagcttgt gaccagcggg ctctcctcgc gcgtcggtcg tgacaacctg gagctggaac
     2221 gactcgccgt cgagatagac gtcgcgcgac gtggtcagcc cgatctcgaa tc
//
LOCUS       DATAQL010000922         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4673820, whole genome shotgun sequence.
ACCESSION   DATAQL010000922 DATAQL010000000
VERSION     DATAQL010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4673820"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..644
                     /locus_tag="VHS97_16010"
     CDS             321..644
                     /locus_tag="VHS97_16010"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX3449761.1"
                     /translation="MSWRVEILDETVAAEISALPADMQARFLRLAERIASAGLESLSE
                     PHVKHLEGKLWELRLTGRDGIARALYVTAIGRRVVVVRAFVKKTQRTPRAEIELALRR
                     AKDIT"
     gene            641..922
                     /locus_tag="VHS97_16015"
     CDS             641..922
                     /locus_tag="VHS97_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3449762.1"
                     /translation="MTIQFEKLKARLLANPKVKAEYDALAPEFEIAAELLRARLRAGL
                     SQAELAARMGTSQSTIARLENGYTLPSTKTLLRYAEATGSKFHVRLSAA"
     gene            2024..3409
                     /locus_tag="VHS97_16020"
     CDS             2024..3409
                     /locus_tag="VHS97_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3449763.1"
                     /translation="MRPKGIGALSFAIAAVGLFYAAAPAMAQEKKITVWFGKGFYKSE
                     DDALLDAIRKFEAKTGIKVELSQYAIQDMIPKTVAALDSGTVPDVAYSDSYDVQAQGK
                     WAFEGKLEDLSDILTPMKPAFAPNTLETAYLYNDVTKKKAYYGFPLKQQSMHVQIWGD
                     MLEQAGFKASDIPTKWQDYWSFWCDKVQPAIRKATGQRIYGVGQPMGVESTDSFQSFY
                     TFMDAYNVKLVDDDGKLLVDDPKVRQGLIGALKDYTDTYLKGCTPPSSTTWKDPDNNV
                     AFHNKTTVMTHNFTISIAAKWFEDSTNPALTPEQRAAGKKAYEETIITASFPNKPDGM
                     PIKYRSDVKTGLIFTAAKNKAEGKEFVKFLLQEENLQPYVEGALGRWFPVTTAGQKSP
                     FWQADRHRKAVYTQFTGGTTPFDFTRNYKFTILNNENVWAKAMNRVVSEKVPVDKAVD
                     ELIARIKQVAG"
BASE COUNT          722 a         1056 c         1039 g          639 t
ORIGIN      
        1 ctgcgctcat gcgggctact tgctactgtc ggaaaggttc tgaacgtctt tttgtctgtc
       61 ataggtcatc cttcgggata acctgtcaca gaaattaccg tgtctcactt acggggtgca
      121 gtccagcact gtcaccgtaa ctcctgtcac cgtaactcct gtcaccgtaa ctccttgcgc
      181 gccttggacg cggcggatgg caattggcga cagcgtaggc ccgccgtctg gcgaggatga
      241 cgaattgcca gtttcccgca ctggcgcagg tcaggatttc acctcttttc tatatctcaa
      301 aattgagata caaaggagta gtgtcctggc gagtcgaaat ccttgacgaa accgtcgccg
      361 cggagatctc cgctcttcca gccgacatgc aggcgcgatt tctgcggctt gccgagcgca
      421 ttgcgtcagc cgggctggaa agcctgagcg aaccgcatgt gaagcacctg gaggggaagc
      481 tctgggagtt gcggctgacg ggccgcgatg gaattgcccg cgcgctgtac gtcacggcga
      541 tcggtcgccg ggtcgttgtc gtgcgtgcgt tcgtgaagaa gacgcagagg acgccgcgcg
      601 cggagatcga actcgcgttg cggcgagcaa aggacatcac atgaccatcc agttcgaaaa
      661 gttgaaggcc cgcctgctcg ccaaccccaa ggtcaaggcc gagtatgacg ccctcgctcc
      721 ggagttcgag atagccgccg agttgctccg ggctcgcctg cgcgcaggct tgtcccaggc
      781 cgaacttgcg gcccggatgg gcacgagcca atccaccatc gcccggcttg aaaatggtta
      841 tacgcttccg agcaccaaga cgctgctgcg ctacgccgag gcaaccggca gcaaattcca
      901 cgtgcggcta tcggcggcct gacgggtcgt tcgcgccgat tgcggacctg taacaaggca
      961 gttcatgtat ccattgaggg caaggcggca ttttcgagcc gtggcctctt gcgccccgcg
     1021 gcgaatttct tcaaccaaat caatttgatt ccacctgtcc agttctcctt ccaaaaatat
     1081 tcctcttccc gctcccccca aatcaacggc agaactcccg ccgtctcatc ccccacgggg
     1141 ggcgttggcc atcgtcacga acgttgggat gggatgcggt ggacgcggca gcgtcgggcg
     1201 cgtttgggat cgcagggcag cctttggctt gtgagcgatc gacctgcgcg cagacgaacg
     1261 gcgctgcttc cgtcttcacc aaagcttcgg cggatgtgca catgccggcg aagcctcttg
     1321 gcgaggacgg gtcgcggacg gtgaagccgt gtggtcctgg cacccgttgc tggtgctaaa
     1381 tcggcggagg tgcgtcggcc caaccgggcg cggacgtgcc gtcaatccgc cgatgacggt
     1441 gacaagacga attcgtcgcc ggggagagcg cggagtaaac cgttaaaacc attcgcgtgc
     1501 gggaatgccg ggtgattccg gtgaacctgc ggtgactacc gtgtgcattt tttgttgcac
     1561 acgggccgtg ggtgcatcgg gcacccggca ttccccacgc cctccatttg caaagggcga
     1621 aagtttcttc aacgacccgg acgcatcgcg ccgcggggtc gtaaagttgc gtctggaacc
     1681 aggatcccgt cattgcgagg agcacttgcg acgacctgtc cgccgaagct acgagcgaag
     1741 gcggaaagca atccatcctt ttcttccgtc gcgcggcacg atggattgct tcgcggagcc
     1801 tgtcatcggg cgcgcattcg cgcgacccgt tggctcgcaa tgacggggag agagtcttcg
     1861 tgccgaccca gtcgctcgca atgacgccac gaggtcacac tacggcaccc ggagccgggc
     1921 gtgttatacg caagacatag attgcccatc tggatcggac cgcggccatg cgctagtctt
     1981 ttgtccgacc ggttaaaaaa atcggcagat cagggagagc aacgtgagac ccaagggaat
     2041 aggtgcgctt tcattcgcga tcgccgcggt cgggcttttc tacgccgcgg cccccgcgat
     2101 ggcccaggaa aaaaagatca cggtctggtt tggaaagggc ttttacaagt ccgaggatga
     2161 cgcgctgctc gatgcgatca ggaagttcga ggccaagacc ggcatcaagg tcgaattgtc
     2221 gcaatatgcc attcaggaca tgatcccgaa gacggtggcg gcgctcgact ccggcacggt
     2281 gcctgacgtt gcctattccg acagctacga cgtgcaggcg caaggcaaat gggcgttcga
     2341 gggcaagctc gaagacctct ccgacatcct cacgccaatg aaaccggcgt ttgcgccgaa
     2401 cacgctggag accgcctatc tctataacga cgtgacgaag aagaaggcct attacggctt
     2461 cccgctgaag cagcagagca tgcatgtcca gatctggggc gacatgctgg agcaggccgg
     2521 cttcaaggcc agcgacatcc cgaccaaatg gcaggactac tggtcgttct ggtgcgacaa
     2581 ggtgcagccg gccattcgca aggccacggg ccagcgcatc tacggcgtcg gccagccgat
     2641 gggcgtggaa tcgaccgact cgttccagtc gttctacact ttcatggacg cctacaacgt
     2701 caagctcgtc gacgacgacg gcaagctcct ggtcgacgat cccaaagtcc ggcaagggct
     2761 gatcggcgcg ctgaaggatt acaccgacac ctacctcaag ggctgcacgc caccctcctc
     2821 caccacctgg aaggatccgg acaacaacgt cgcgttccac aacaagacga ccgtgatgac
     2881 ccacaacttc acgatctcga tcgcggcgaa gtggttcgaa gattccacca acccggcgct
     2941 gacccccgag cagcgtgccg ccggcaagaa ggcttacgag gaaaccatca ttaccgcgtc
     3001 cttccccaac aagccggatg gaatgccgat caagtaccgc tccgacgtca agaccggctt
     3061 gatcttcaca gctgcgaaga acaaggcgga aggcaaggaa ttcgtcaaat tcctgctgca
     3121 ggaggaaaac ctccagccct atgtcgaagg cgcgctcggc cgctggttcc cggtcaccac
     3181 cgccggtcag aagagcccgt tctggcaggc tgaccgccat cgcaaggcgg tctataccca
     3241 gttcacgggc ggcacgacgc cgttcgactt caccaggaac tacaagttca cgatcctgaa
     3301 caacgagaac gtctgggcca aggcgatgaa ccgggtggtg agcgagaagg tgccggtcga
     3361 caaggccgtc gacgaactga tcgcccgcat caagcaagtc gcgggctgac attagccata
     3421 ccggccgcag cgcgcggccg gcatcttgtt gtgagc
//
LOCUS       DATAQL010000923        11557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_553566, whole genome shotgun sequence.
ACCESSION   DATAQL010000923 DATAQL010000000
VERSION     DATAQL010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553566"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..670)
                     /locus_tag="VHS97_16025"
     CDS             complement(140..670)
                     /locus_tag="VHS97_16025"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3449764.1"
                     /translation="MPGASAQPEVDARSGQAVVQWILPAGSRGLLAATVMGVAMGLAC
                     TSYSVPGSAGGAIVAAPDLLIDLNTAPPPVLETLPHVGQTLVRQLVAARELRPLASLE
                     ETASRVRGLGPTTVARLRPYLRFGDSAPGRGEPKWSADQDRSDGKTPSIVRKRNRSRK
                     PTAVTLQSRLVAQRLE"
     gene            complement(836..2065)
                     /locus_tag="VHS97_16030"
     CDS             complement(836..2065)
                     /locus_tag="VHS97_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449765.1"
                     /translation="MGNALLVDDDRPDTDASTSLAAEPDVASPGDRSAPRRRFGVHRL
                     QPTANRLLRAIASAAEWLFGLVSLVLGLSMLAALPLVQFASLGYFLESSARVARTGRL
                     RDGFIGVRRAARVGGLAVAIWLSLVPAWLVSSYAHSAQLIDPGGPVARKWQVALVVVT
                     VVSLLHIAASCARGGRFRHFLWPVGHPFWLYRQLRDGVLYSEARDSLWAFVARLRVPY
                     YFRLGLIGFLGTLAWLILPAILIGATQRFPVLGALGALLLAFIVPFLPFLQVRYAVEG
                     RVSALFSRRAIRDRFRRAPWAFAFSLLILLVAAIPLYLLKLEMIPREAAWLPSLVFVV
                     FLAPARLLTGWAYARSERRDRPRHWVFRILGRLAIIPTAALYVLIVFLAQYTSWGGVS
                     SLYEQHAFLLPVPFLNM"
     gene            complement(2210..3526)
                     /locus_tag="VHS97_16035"
     CDS             complement(2210..3526)
                     /locus_tag="VHS97_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEX3449766.1"
                     /translation="MRLCLVEDLAVCAIEPLTLTRPVHELLLGNTTLASKIARAFGVG
                     PGPQRRSCLIRSHLVEVQRQRDPHMAVNDRDWLARAPLVAANGRWVPPVGFEIPNEYG
                     PWVGLCDGRPACARIGPDDAVSLEPLGVDDWFERMVAKHGGLEVGGEWIDRPWDLVTK
                     NEAHLERDFAGAGKVGVTNRHLAKLELVGPVDRLWIHESARIDPYTVFDTTNGPIVLG
                     PNVWVQPFTRIEGPCSVGADSQLFRANIRGCVSVGPNCRIGGEVEAAIVHGHSNKYHE
                     GFLGHAYVGEWVNLGAITSNSDLRNDYGEVFVPLQGDPVPTGQKKVGCFIGDHTRTGM
                     GSMLNTGTAIGVMCNVLPAGILLPKHVPSFTAVSYGRVAPGFPLDQLFETARTVMGRR
                     GKTFGEAEEQLYTTLYEQTRLERERAFQRTAARRGDHWPIAQAARL"
     gene            complement(3766..5535)
                     /locus_tag="VHS97_16040"
     CDS             complement(3766..5535)
                     /locus_tag="VHS97_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX3449767.1"
                     /translation="MWIQRYDPLGHWVVSTLVAALPVLVLLGLLAAGRASAWKAALLG
                     LLTASGIAWLVFGMPATMVAAAALVGVVFALLRIVWLIVAAVFLYDITVATGQFEIMK
                     ASVARLSADRRLQAVLVAFCFGAFIEGAAGFGAPVAISAAFLVGLGFQPFEAALLCLI
                     ANTAPVAWGGIGTPILTLSKVTGLDVHALSATAGRILPIMSLVIPFWLVRAMTSWRET
                     AAVWFPLTVIGGTFASVQFVWSNYVGFELVDIVASVASMAAGVIVIRFWRPRRTWRFE
                     RDAGDSESQEFEDSQRVQKASGDLSLSRITRAWMPFALLTMTVLVWGVPAIKPMGTPA
                     VKDWLDQRLSWKPELPWLHLKTAKGALVTGHNTPQPEDFEKAQVEIVPLSSTGTAVFL
                     AAVISGSWLGLPATRMAGILVGTVRRMIPAIAAILFMLALGFVTKYSGMDAVLGLAFT
                     RTGPLLYPIFGTLLGWLGVALTGSDTSSNVLFGNLQKITAAKLGLNPILMASANSTGG
                     VMGKMIDAQSIVVAAAATGEGGREGELLRAVFWHSILLALLVGAIVWIYAHVLPGYVP
                     LQPTLSVGAALPFQAAHASAHAV"
     gene            5825..6205
                     /gene="raiA"
                     /locus_tag="VHS97_16045"
     CDS             5825..6205
                     /gene="raiA"
                     /locus_tag="VHS97_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX3449768.1"
                     /translation="MQIEISTRHGGLGSEQQRYLHDKAEKLLKYFARLMAIEVAVDHG
                     KHGWEVEILVSAEHKHDFVAKEIGSTPEAAMDLCVHKIEQQLRRYKEKVQNHKGDVTH
                     GGSSPRSFEHPPAPVETPTDPDDV"
     gene            6371..6841
                     /locus_tag="VHS97_16050"
     CDS             6371..6841
                     /locus_tag="VHS97_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX3449769.1"
                     /translation="MKLMDFVVGESIIVDLKATGKEEAIREMVGSLHRAGRLGNDDLE
                     SVIRAILGREELGSTGIGQGVAVPHTRHPTLNRLIGTVALSRRGVNFAALDGEPVDIF
                     FLLVSPQNQPGDHLRALENISRHLKEERFVGFLRQARSRDNVIDVLEGADQGST"
     gene            6990..7322
                     /locus_tag="VHS97_16055"
     CDS             6990..7322
                     /locus_tag="VHS97_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX3449770.1"
                     /translation="MSHETTVARRQVEVTNSLGLHMRPANKFVELALQYQAEIRVHYN
                     GNEYNGKSILDLTSLAAECGTLLDVEARGPDAAAAIEALADLVSARFHENENGDPTAV
                     EPRTESAS"
     gene            7319..9256
                     /gene="ptsP"
                     /locus_tag="VHS97_16060"
     CDS             7319..9256
                     /gene="ptsP"
                     /locus_tag="VHS97_16060"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX3449771.1"
                     /translation="MNLRDREFPDRRGARREVAPRSSRSAPALALSARSLRLESEMPP
                     PVPVTTSPSSSRPMQILRGQAVSPGIAIGPVVVLNPRGVRLPPRSIGSGTIAAELERL
                     DRGIAAAHSAAGQDEGEARARLGPQYADILAAHCRMISDPTLRGDARKIIEEQHASAE
                     HAVIDVLEGHASRLERLSGSRLAARAADVRDIEARILTHLIGEQPRSFQDNLAAPAIL
                     LAHDLSPSEAAGIDPRLVPGFATEGGGRASHTAIVAAALEIPAVVGLGKFLDRAQHCH
                     MAIIDGDEGLLILDPDLVTQERYHKTAAERAAWFHVLTQQTDLPAETADGTRIELWGN
                     IEFGGEVEACLERGAVGVGLFRTEFLFLNALTPPSEEEQFQVYASVVRSMRGRPIVIR
                     TLDLGADKLPLYRDATQDESNPALGLRSLRLSLSEPGLFRPQLRALLRASTLGELRIL
                     FPLVSTLAELRAARAVLDDVAAELLAEGHAVREKLPVGVMVEVPAAALMADHLAKEVD
                     FFSIGTNDLIQYTLAVDRTNETVADLYCAADPAVLRLIAMVVDAARNHGIEVSVCGTM
                     GGELLYTTLLLGLGLKHLSMPPHQLPEIKRLIRGMRIEVARQVAEDVLLLKTAEEVVE
                     RLTCALRQISPERHNRSADST"
     gene            9601..10224
                     /locus_tag="VHS97_16065"
     CDS             9601..10224
                     /locus_tag="VHS97_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX3449772.1"
                     /translation="MRLRFAKCGDLRLVSHRDIMRCLERMLRRAQVPMAVTQGFNPRP
                     KMTFALALGLGIEGQREVVDLEFSEPLEPSELLSRLTRVAPPGFDWIDVRPLPANATP
                     PHPRTVEYCMPVLDERRAMAQSNLQSLLTSASWLLTRRRPNRESTFDLRPELVAAELT
                     AEGLLRFRLKVSPDGSARPEELLEALALRDLLDHGTVLTRTDVELDW"
     gene            10435..>11557
                     /locus_tag="VHS97_16070"
     CDS             10435..>11557
                     /locus_tag="VHS97_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449773.1"
                     /translation="MKKEMLINVLQPEECRIAIIEDGILEELYVERTSHESYTGNIYK
                     GRIVNLEPSIQAAFVDFSVGRNGFLHVSDVEPQYFDRPQGMHDEAGPDPRDRERKRDP
                     RRGRGRDDRRRTAGPEPLEPPPGGVPPAPPRLEERPRDRGRERDKERGRERERPDLSI
                     RGRRDRSEPPSRRFGEGLVEPAEEPPTPPRAVEAAPIQPYSPFEDDTFRSTQESEKSL
                     PAGWPETAGHSWDEDLNRVSEPEPEFGRASDPFFEREPSRDELDEPPARNTRAVEPFD
                     PDLPPSRTQRSRGPSTTPRPGELERPERGRHRLPPSSPSSLDEWQGGDTGLADPWLEP
                     PRERDPRRGRGRGRSAPPSGRPRPIPPAGREGLPIPEIEP"
BASE COUNT         2395 a         3574 c         3603 g         1985 t
ORIGIN      
        1 cgagcctcag ggaattgggt ggcccggtta gaccgaacat agttgcaagg gcgcgcaatt
       61 ccaagtcgtt cagcttgaga gcgttggcca gtccgagtag ttcctcgatc ttgaagatca
      121 cgatctccga atacgcatct cattccaaac gttgcgcgac aagccgtgac tgtagagtta
      181 ccgcagtcgg ttttcgcgac cgattccgct tgcggacaat cgaaggtgtt ttgccgtccg
      241 atcggtcctg atcggcgctc cacttaggct cgcctcgccc tggcgccgag tcgccaaatc
      301 ggaggtatgg ccggagccgg gccacggtgg tgggcccgag gccgcggact cggctggccg
      361 tttcttcgag ggaagcgaga ggcctgagct cccgggccgc aaccaactgt cgcaccaatg
      421 tctggccgac gtgaggcaac gtttccaaca ccggcggcgg tgcggtgttc agatcaatca
      481 acaagtcggg cgccgcgacg atcgccccgc cggccgagcc cggcaccgaa tagcttgtgc
      541 aagccaaacc catcgcgacg cccatgacag ttgcggccag taagcctctg gacccggcgg
      601 gcaggatcca ctggacgacc gcctgcccgg acctggcatc aacctcgggc tgcgcggaag
      661 caccaggcat gctggacatc tgtagcgtaa cccttgagtt ggcttgccct taagtctcgg
      721 caatcctgct tcgatagcct acaatcccgc tcgcgttcaa tcaagcagtg cgcgaaagat
      781 gccgacgtac gcacagcgaa atcgcatcag atggatacga tcggggcgtg tgacgtcaca
      841 tgttgagaaa tggcacggga agcaagaagg catgttgctc gtagaggctg ctgacgccgc
      901 cccatgacgt gtactgggcg aggaagacga tcaagacgta gagtgcggcg gtggggatga
      961 ttgcaagccg gccgagaatg cggaagaccc agtgtcgggg acggtcgcga cgttcagaac
     1021 gggcgtaggc ccaacccgta agcagccgtg ccggcgcgag gaagacgacg aaaacgagac
     1081 tcggcagcca ggcggcctcg cgcgggatca tctcgagctt cagcagatag agtggaatcg
     1141 ccgccaccag caagatgagg agcgagaatg cgaaagccca gggggcgcgg cggaatcgat
     1201 cgcggatggc tctcctggaa aaaagagccg acacccggcc ttcgactgcg tagcgaacct
     1261 gcaagaaagg aaggaacggc actatgaatg ccagaaggag cgcaccgagc gctccgagaa
     1321 caggaaatct ctgggtggcc ccgatcaaga tcgctggcag gatcaaccac gcgagcgttc
     1381 ccaggaatcc gatcaagccg agccgaaaat agtacggcac ccgcagccgc gccacaaagg
     1441 cccaaagact gtcgcgcgcc tcagagtaca agacgccgtc acgcaactgg cggtacagcc
     1501 agaatgggtg gccaaccggc cagagaaagt ggcggaaccg tccacctcgc gcacacgacg
     1561 cggcgatgtg caacagcgat acgacggtca cgaccacaag tgcgacctgc cattttcgcg
     1621 cgacgggtcc tccaggatcg atcaattgcg cggagtgcgc gtatgatgaa accagccacg
     1681 cgggcacaag cgacaaccag atggctaccg caaggccgcc gacgcgcgct gcccgcctca
     1741 cgccgatgaa accatcgcgc aaacggccgg ttcgcgccac ccgcgcggag gattcgagga
     1801 aatatcccag acttgcgaac tgaactaacg gcaacgccgc gagcatcgac aaacccagca
     1861 cgagcgacac caagccgaaa agccattcgg ccgcagaagc gattgctcga agaaggcggt
     1921 tggccgtagg ctgaaggcgg tgaactccga accgtcgcct gggcgccgaa cggtccccgg
     1981 ggctggcaac gtctggctca gccgcgagcg acgtggacgc gtcagtatcc gggcggtcgt
     2041 cgtcaacgag caaagcgttt cccatgctgg ctactacccc cgcggatgat ccacggtttc
     2101 gcgtaccgcg gcaaggggat aagccaacat ttggattgcg gccagcgtgg acgaccaaac
     2161 ctaccatccg gcgagggaag cagatctggg acgtgccttc atcgtcgcgc tagagccggg
     2221 cggcctgggc gatcggccaa tggtcaccgc gccgggcggc cgtgcgctgg aaggcccgct
     2281 cgcgctcgag gcgcgtttgc tcgtagagcg tcgtgtaaag ttgttcctcg gcttcgccga
     2341 aggtcttgcc gcggcgcccc atcacggttc gggcggtctc gaaaagctgg tcgagaggaa
     2401 agccgggagc gactctgcca tacgacaccg ccgtgaatga cggcacgtgc ttgggcaaga
     2461 gaattccggc cggcaagaca ttgcacatca caccgatggc agtgcccgtg ttgagcatgc
     2521 tccccatccc ggtgcgtgtg tggtcgccaa taaagcagcc gaccttcttc tggcccgtgg
     2581 gaacggggtc gccttgcagc ggaacgaaaa cctcgccgta atcgttgcgt aaatcgctat
     2641 tggaggtgat cgcccccagg ttgacccatt caccaacata cgcgtggccc aggaagccct
     2701 cgtgatactt gttggaatgg ccgtggacga tcgcggcctc gacctcgccg ccgattcgac
     2761 agttcggtcc gacggagaca catccgcgaa tgttcgcgcg aaatagttgc gagtcggcgc
     2821 cgacagaaca aggcccctcg attcgcgtga atggttgaac ccatacgttg gggccgagga
     2881 cgataggacc attagtcgtg tcgaagaccg tatagggatc aatccgggcc gactcgtgaa
     2941 tccagagccg atcgactggg cccacaagct cgagtttggc gagatgccgg ttggtcacgc
     3001 cgaccttgcc cgccccagcg aagtcgcgtt ccaggtgtgc ctcattcttg gtgacaaggt
     3061 cccagggacg gtcgatccat tcgccgccga cctccaatcc gccgtgcttg gcaaccatcc
     3121 gttcgaacca gtcatcgacg cccagtggct cgagactgac ggcgtcatcg ggtccaatcc
     3181 gcgcacaggc ggggcggcca tcgcaaaggc caacccaggg cccatattca ttcggtatct
     3241 cgaagccgac cggcggcacc cagcgcccat tggccgcgac caacggcgcg cgcgccagcc
     3301 aatcgcgatc attgaccgcc atgtgcgggt cgcgctggcg ctgcacttcc acaaggtgag
     3361 accggatcaa acagcttcga cgctgcggtc ccggtccgac cccgaaggca cgtgcaatct
     3421 tgctcgcaag cgtggtattg ccaagcagca gttcatggac ggggcgagtc aacgtcaacg
     3481 gctcaatggc acagacggcc agatcctcga caaggcagag acgcatagca cctcccggtc
     3541 gttcagatgg gcaagaagcc tgcaaaaatg gcgtcaatca gcgaccaaac caggtgaaaa
     3601 cttgtgatcg gtgggccgcg tcacccacct gaggacgttg attaatctcc tctcggaaca
     3661 aattttatca aaaagagtca aactcggcta gatgaatacg tccaatgctg ctcgcgtaca
     3721 tgattgcgtt gtatttattc atcacgcatg gcctgcggcg gcaaactaca cagcatgggc
     3781 ggacgcatgc gccgcttgga aaggcagcgc ggcgccaacg gataaggtag gctgcagagg
     3841 cacatagcct ggcaggacat gagcgtagat ccacacgatc gcacccacca aaagtgccag
     3901 aagaatgctg tgccagaaga cggcgcggag caattctcct tcacgcccgc cctctccggt
     3961 ggctgcggcg gcgacgacga tcgattgagc gtcgatcatc ttgcccatca cacccccggt
     4021 ggaattggcc gaggccatga ggatggggtt taaccctagt tttgccgcgg taattttttg
     4081 caggttgccg aagaggacgt tgctcgatgt gtccgagcct gtcagtgcca ctcccagcca
     4141 cccaagcagg gtgccaaaga ttgggtagag caacggaccg gttcgggtga acgccaagcc
     4201 caaaacggcg tccatccccg aatacttggt cacaaatccc agcgcgagca tgaacaggat
     4261 cgccgcgata gcggggatca ttctgcgcac ggtcccgacc aagatcccgg ccattctggt
     4321 cgcgggcaga ccgagccatg agccgctgat gacggcggcc agaaatacag ctgtgccagt
     4381 tgacgacaag ggaacgatct cgacctgagc tttctcaaaa tcttccggct gcggtgtgtt
     4441 gtgcccggtg acaagtgctc ccttggccgt cttgagatga agccatggga gctccggctt
     4501 ccacgaaagc ctctggtcga gccagtcctt gactgccggg gttcccattg gcttgatcgc
     4561 agggacaccc cataccagca cggtcatggt caagagcgcg aagggcatcc aggcccgcgt
     4621 gatccgtgac agtgacagat ctccggatgc cttttggacg cgctgggagt cctcaaactc
     4681 ttgactctcg gaatccccgg catcccgctc gaagcgccag gtacgccgag gacgccagaa
     4741 gcggatgacg atcactccag ccgccatgct ggcaaccgac gcgacgatgt cgacgagctc
     4801 gaagccaaca tagtttgacc aaacaaattg gaccgaggca aacgttccgc caatgacggt
     4861 gagcggaaac cacacggccg cggtctctcg ccatgaagtc atcgcgcgca ccagccaaaa
     4921 aggaatcacg agcgacatga tgggcaagat acgtccggcg gtggcgctca aggcatggac
     4981 atcgagtccg gtgaccttgc tcagtgtcag gatgggagtg ccgatgcctc cccaggcgac
     5041 gggtgccgtg ttggcaatca ggcagagcaa ggctgcctcg aagggctgaa aacccaatcc
     5101 gaccaggaag gccgcggaga ttgcgaccgg ggcaccgaag ccggcggctc cctcgataaa
     5161 cgccccgaaa caaaaagcga ccagcaccgc ctgcaagcgg cgatccgccg atagccgcgc
     5221 caccgaagcc ttcatgatct cgaactggcc ggtcgccaca gtgatgtcgt aaaggaacac
     5281 ggcggccacg attaaccaga caatccgcaa cagagcgaat acgacaccga ccaaggcggc
     5341 ggcggcgacc atggtggccg gcatgccgaa tacgagccat gctatgccag acgccgttaa
     5401 cagccccaag agggcggcct tccatgcgct ggcccggccg gcggccagca aacccaaaag
     5461 caccagtacc ggcagcgccg cgaccagagt cgagaccacc cagtgtccta gcggatcata
     5521 ccgttgaatc cacacggctg cttgttctcc tttttgattg ccatagcaag cacgaccgcg
     5581 ggaacacgcg gacacggcgc ggcaaaggtg tcgtgtacga cgtacgatac cggccgatgg
     5641 gggggtgggg tagtgccctc cgcatgatgt tgggggtgtc gaggttgcac gaggatcgcg
     5701 gccaaactaa cggacggctt tgatggggtc gtgccttgac cgcaacgcga gaatagagtt
     5761 aattttaatt gtcgcttcgc ccgtcacgac gggaagtcaa gacgacctat caaggagtac
     5821 gtgggtgcaa atcgagattt cgacccgtca cggagggttg ggttccgagc aacaacgtta
     5881 cttgcatgat aaggccgaga agcttctcaa gtattttgct cgcctgatgg cgatcgaagt
     5941 cgcggtcgat catggcaagc atggctggga ggtcgaaatt ctggtctcgg ccgagcacaa
     6001 gcatgacttt gttgccaagg agatcgggtc tacgccggaa gcggcgatgg atttatgcgt
     6061 tcacaagatc gagcagcagc tgcgccgcta caaagagaag gttcaaaacc acaaaggcga
     6121 tgtcacccat ggcggatcgt ctccgcggtc gttcgagcat ccgccggcac ctgtcgagac
     6181 gccaaccgat cccgacgacg tctgacgccc atctttgctt gaaccgatcg tctcgcggca
     6241 ttcgcgagac gcttcgcggc ggggggctgg tcgaagatgg tcgcagtggc gttgacggat
     6301 cgccagtgcg acgtagactg gaagccggtc atcgaatcat gcgatcggcg gcccgtcgtt
     6361 ggaggggcgg atgaagttaa tggactttgt tgtcggtgag tcgatcatcg tcgacttgaa
     6421 ggctaccggc aaggaagaag cgattcgcga gatggttgga agcctgcacc gcgctgggcg
     6481 gttgggcaac gacgacctgg agagcgtgat tcgcgcgatc ctcgggcgag aagagctcgg
     6541 ttccacgggg atcggccagg gtgtggctgt tcctcacacg cggcacccga ccttgaaccg
     6601 cttgatcgga accgtggcgc tatcgcggcg cggggtcaat ttcgcggcct tggacggtga
     6661 gccagtcgac attttcttcc tgctggtgtc gccccaaaac cagccgggag atcacttgag
     6721 agcgctcgag aatatttcac ggcatttgaa agaagagcgg ttcgtggggt ttttgcgaca
     6781 ggcacggtcg agagacaacg tgattgatgt actggaggga gccgaccagg gctctacgta
     6841 gtcgtcgcta gagcggacga cggctgatcc cgatagaaga ggacgctcgc cgacgaaaat
     6901 cgatacgaac tacagactgc cggccggttg ttttggtccg gcaacgcccg gtgttcgctt
     6961 gtccccggcc caggccgatc gagccgccga tgagccacga aacgaccgtc gcccgtcgcc
     7021 aagtcgaggt tacaaatagc ctcggactgc acatgcggcc cgcgaataaa tttgtcgaac
     7081 tagcgctgca gtaccaggcc gaaatacgcg tgcattacaa cggcaatgaa tataacggca
     7141 agagcattct cgatctgaca tccctggccg ccgaatgcgg cacccttctc gatgtggagg
     7201 cgcgcgggcc cgacgcggcc gcggccattg aagcgctggc cgacctggtt tcggcccggt
     7261 tccacgagaa tgagaatggg gatccgaccg cggtcgagcc acgcacggag tcggcttcat
     7321 gaatttgagg gaccgcgaat tccccgaccg gcggggcgct cggcgcgaag tggcgccgcg
     7381 ctccagtcga tcggcgccgg ccctcgctct ttcagctcgc tctttacgcc ttgaatccga
     7441 aatgccccct cctgtaccag tgacaacgtc gccgtcttcc tccaggccca tgcaaatctt
     7501 gcggggccag gcagtcagcc cgggtatcgc gatcggcccc gtggtcgtgc tcaatccgcg
     7561 cggcgtgcgg ttgccgccgc gttcgattgg gtccgggact atcgccgcgg agctcgagcg
     7621 ccttgaccgc gggatagcag ccgcacactc cgcggctggc caggacgaag gcgaagcgcg
     7681 cgcccggctg ggcccgcaat acgccgacat cctggctgct cattgccgta tgatctccga
     7741 ccccacctta cgcggcgatg cgcgcaagat catcgaggag caacacgcct cggcggaaca
     7801 tgccgttatc gacgtgctcg agggacacgc atctcggcta gagcggctct cgggctcgcg
     7861 tctggcggct cgtgccgcgg acgtccgcga catcgaggct cgcatcctca ctcacttgat
     7921 cggagagcag cccaggtcgt ttcaggacaa tctggccgcg ccggcgatct tgctggccca
     7981 cgacctgagt cccagtgaag cggccggaat cgatccgcgg ctcgtcccgg gcttcgcgac
     8041 cgaagggggg ggccgagcca gccacacggc gatcgtcgcc gccgcactcg agatcccggc
     8101 cgtggtgggg ttgggtaagt ttctcgatcg cgcccagcat tgccatatgg cgatcatcga
     8161 tggcgacgag ggtctgctga ttctcgaccc cgaccttgta acccaggaac gatatcacaa
     8221 gacggccgct gaacgagcgg cctggtttca cgtcctcaca cagcaaaccg acttgcccgc
     8281 cgaaaccgcg gacggcactc gcatcgaact ctggggaaat atcgagtttg gcggcgaagt
     8341 cgaagcgtgc ctcgaacggg gcgcggtcgg cgtcggcttg ttccgcacgg aattcctgtt
     8401 cttgaacgcc ctaacgccgc cgagcgaaga ggagcagttc caagtctatg catccgtcgt
     8461 ccgctcgatg cgcggccggc cgatcgtaat caggacgctc gatctgggcg ccgacaagct
     8521 ccccctttat cgagacgcga cccaggatga gtcgaatccg gccttgggtc tgcggagctt
     8581 gaggctgtcg ctctcggaac ctggtctttt ccgtccgcag cttcgcgccc tgctccgcgc
     8641 gagcactctg ggagagctgc gaattctgtt tccgctggtg tcgacactcg ccgaactgag
     8701 ggcggcgcgg gccgtgcttg acgacgttgc cgccgagcta ttggcggaag ggcacgctgt
     8761 ccgcgaaaag ctgcctgtcg gcgtgatggt ggaggtaccg gccgccgctc tgatggcgga
     8821 tcatctggca aaggaggtgg atttcttttc tatcggaacc aacgatttaa ttcagtacac
     8881 gctggctgtt gaccggacca acgaaacggt tgccgactta tactgcgccg ccgatccggc
     8941 cgtgctccgc ttgatcgcca tggtggtcga cgcggcgcgg aaccatggaa tcgaggtcag
     9001 tgtctgcggc acgatgggtg gggaacttct gtacacaacg ctgttactgg gcctgggcct
     9061 caagcacttg agcatgcctc cgcaccaact gcccgaaatc aaacgattga ttcggggaat
     9121 gcggatcgaa gtcgcgcggc aggtggccga ggacgtgctc ttgctcaaga cggccgagga
     9181 agtggtcgaa cgtctcacat gcgcgctgcg tcagatttct cccgaaaggc acaaccgatc
     9241 ggccgactcg acctgaacga acgcaattcg ccgtgaattc gcggcgggaa ccgtcggttc
     9301 aggctctctc gtgatggtgg gcaggcgtac ctgtggaaaa agcatttagg gggtccgctc
     9361 cgccgctctc attggtccca aggccagacg atttctttta actgcacaac tcggggcaaa
     9421 ccgctccgaa ccatctcgat ccccaagcag gcgtttctct gcctgacgat cgaagacggc
     9481 caagaagcgg cgaacccgaa atagaagcgc ggctggaaaa acgagctcgt atcgtgctcg
     9541 ggccgcgagt gaggtcgagc gcggcgtgcg cgtccgcggg atatgacaga agccaccaaa
     9601 gtgcgtctcc gatttgcgaa gtgcggggat ctccgcctgg tcagccatcg tgacatcatg
     9661 cgctgcctcg agcgaatgct ccgtcgcgcg caggttccga tggccgttac tcagggattt
     9721 aacccccggc ccaagatgac ctttgctttg gccctcgggt tgggtatcga ggggcagcgc
     9781 gaggtcgttg acctcgagtt ttcggaacct ctcgagccgt ccgagctgct ctcgcgactc
     9841 acaagggtag ctcctcccgg tttcgactgg atcgatgtcc gcccgctccc cgcaaacgcc
     9901 actcctcctc acccccggac ggtcgagtac tgcatgcctg tgctcgatga gcgccgtgcc
     9961 atggcgcagt ccaacctgca atcgctcttg accagtgcaa gctggctatt gacccggcgc
    10021 aggcccaacc gcgagtcgac ttttgacttg cgtcccgaac tggtcgcggc cgagttgacg
    10081 gccgagggct tgctccgatt tcgcttgaag gtgtctcccg acggctccgc tcgtcccgaa
    10141 gaactgctcg aggcgctcgc gcttcgcgac ctcttggacc acggaaccgt gttgactcgc
    10201 actgatgtgg agctcgactg gtgaacttct cgaagagggt cgtcgctcga atggaaagac
    10261 gcaaggcgcc ccgcttgacc tctgcaactt cgatcatgtt tcggccctcc aggggcctaa
    10321 attcgagcag tggaccacag acgcaattca ccggaaaagc tcagccccct tccatttcat
    10381 ccccaaccga acacggacgc cctaatgtcg gcgtcagtcc cggaaagagt tcgcatgaag
    10441 aaagaaatgc tgatcaatgt tcttcagccc gaagaatgcc gaatcgccat cattgaggac
    10501 ggcattctcg aagaacttta cgtggagcga accagccacg agagctacac gggcaacatc
    10561 tacaagggcc gaatcgtcaa ccttgagccg tcgatccagg ccgcctttgt cgacttttcg
    10621 gtcggccgaa acggctttct ccatgtctcg gatgtggagc ctcaatattt cgatcgcccc
    10681 cagggtatgc atgacgaggc gggccccgac ccgcgtgacc gcgagcggaa gcgcgaccct
    10741 cgtcgcggcc gcgggcgcga cgatcgccgg cgcacagccg gcccggaacc gctcgagccg
    10801 ccacctggag gcgtacctcc tgcgcctccc cggctggaag agcgaccccg tgaccgggga
    10861 cgcgagcgcg acaaggagcg cggccgcgag cgcgaacgcc ccgatctgtc gatccggggc
    10921 cggcgcgatc ggtccgagcc gccgtcgcga cggttcggcg aagggctggt cgagccagcc
    10981 gaagagccgc ctacaccacc acgggctgtc gaggctgcgc caatacaacc ctattcgccg
    11041 ttcgaggacg atacgtttcg atcgactcag gaaagcgaaa agtctttgcc ggcaggctgg
    11101 cccgagaccg ccggccattc gtgggacgaa gatctgaacc gagtgagcga gccggaacca
    11161 gagttcggtc gcgcttcgga cccgtttttt gaacgggagc catcgcgcga cgaattggac
    11221 gagccgccgg cgcgaaatac acgcgcggtc gaaccgttcg accccgacct gcctcccagc
    11281 cgcactcaac ggtcgcgtgg cccctcgacc acacctcgcc ccggcgagct cgagcgacca
    11341 gagcgtggac gacaccgtct cccgccttca tctccatcct ctctggacga atggcaaggc
    11401 ggcgacaccg gcctggcgga tccttggctc gagccgccgc gcgagcggga cccgcgccgt
    11461 ggacgagggc gagggcgatc ggcacccccg tcggggcggc caagaccgat accgccggcc
    11521 gggagggaag gattgccgat tccggagatc gagccgg
//
LOCUS       DATAQL010000924         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4217002, whole genome shotgun sequence.
ACCESSION   DATAQL010000924 DATAQL010000000
VERSION     DATAQL010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4217002"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VHS97_16075"
     CDS             <1..387
                     /locus_tag="VHS97_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX3449774.1"
                     /translation="RSTMPAFTHHIFVCGNVRAPGHKRGCCDPDGNQTLREAFKKELK
                     TAGFGPLARANHAGCLEQCEHGPTVVIYPQGIWYGGVKIQDVPRIVEKTILGGEILND
                     LMIADVCLNNPDCPHRADPARRESRG"
     gene            380..1237
                     /gene="panC"
                     /locus_tag="VHS97_16080"
     CDS             380..1237
                     /gene="panC"
                     /locus_tag="VHS97_16080"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX3449775.1"
                     /translation="MVEIVESPADLRRKVSDARAKSKKIGLVPTLGALHDGHLRLIRQ
                     CGGEADFVVVSIFVNPTQFGAGEDFNRYPRPLEDDLRQCESAGADLVFVPSVPTVYPH
                     GINSTFVEVPGLSNVLEGASRPGHFRGVATVVLKLLEMVRPDLAVFGQKDYQQQLLVG
                     RMIADLHVPVQLGICPTVREPDGLALSSRNRYLNPQQRQAAVVLNRALERAQQAVDAG
                     ERRGDRIRQILHETLKSESTADLDYAEVADAETLERLGDIGKSTRAVALVAARFGTTR
                     LIDNTLLTT"
     gene            1297..2133
                     /gene="lgt"
                     /locus_tag="VHS97_16085"
     CDS             1297..2133
                     /gene="lgt"
                     /locus_tag="VHS97_16085"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX3449776.1"
                     /translation="MRQVLFTIPIFGGLKVFGYGALLVVAFVSSTWLAYWRAKRERLD
                     PEVIGDMSFWVFLFGLMGARLFYCVEYWGDDIKSLWDVVQYWKGGVVYYGGIVGGVAA
                     FFVFRALRPFPLRPYMDVLAPSIAIGTLFGRLGCFMNGCCFGDICRLPWAVSFPAYSP
                     PWVQHVKSGLIPANALNSLPVHPTQIYSAIDALVIFLLLTAYYPLRRRDGEVIGLLML
                     VYPISRFAIEYLRNDEGVFYGGMTISQTISVILLLLACVYWAWLSSLPKGLYRDLATP
                     SE"
     gene            complement(2174..3196)
                     /locus_tag="VHS97_16090"
     CDS             complement(2174..3196)
                     /locus_tag="VHS97_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3449777.1"
                     /translation="MPSDLDRVEPSLNSIAAAQRGLRQLIERMEDLVVGQRVLLERLV
                     IALLAGGHVLIEGVPGLAKTRAVRALAQALDLPFRRIQFTPDLLPADLTGTQIYRPAT
                     GTFDVRPGPIVTSVLLADEINRAPAKVQSALLEAMQEGQITVGDETIFLPDTFWVLAT
                     QNPIEHEGTYPLPEAQLDRFLMKLVVGYPDRSSELLMLDLPSVSDTPRPDLDSRTSDE
                     PPLYGPDDVLQFRALAASIRIAPAIKEYIVDLVRATRDPASFGLAMAPLLELGASPRA
                     TIALVRAARAHALLAGRDFVTPHDVKSLARDIMRHRILVSFEADAEGLSADDILQRIL
                     DHIPVP"
     gene            complement(3180..>3529)
                     /locus_tag="VHS97_16095"
     CDS             complement(3180..>3529)
                     /locus_tag="VHS97_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449778.1"
                     /translation="TASSNKPVAAPVPPTAPSASDDEYERQPDDEAEEDEPFYQEDDE
                     DEEDDETLAEDESTVVDDVPEEFLDPSNPFYAAKKAQKEQAGPAKHEFKDTSDAASDI
                     LRRLMERRRAERS"
BASE COUNT          642 a         1008 c         1124 g          755 t
ORIGIN      
        1 aggtcaacca tgcctgcgtt cacccatcat atttttgtat gtggcaacgt gcgtgcgccg
       61 gggcacaagc gagggtgttg tgatccggac ggcaatcaga cattgcgaga ggccttcaag
      121 aaagagctca aaaccgctgg attcgggccg ctggcgcgcg cgaatcatgc cggctgcttg
      181 gagcagtgcg aacacggccc gacagtggta atttacccac agggaatctg gtatggcggc
      241 gtcaagatcc aagacgtgcc acggatcgtc gagaagacga tactgggcgg cgaaatcttg
      301 aacgatctca tgatcgccga cgtctgcttg aacaatcctg attgtcccca ccgcgcggat
      361 ccggcccgga gagagtcgcg tggttgaaat tgtcgaatcg cccgcggatt tgcgtcgcaa
      421 ggtctcggac gcgcgggcaa aaagtaagaa aatcggcctc gtcccgacat tgggcgcgtt
      481 gcacgacggc cacctgcggc tgatcaggca atgcgggggg gaggcagact ttgtcgttgt
      541 ctcgattttc gtcaacccaa cccagttcgg cgctggcgag gatttcaacc ggtatccgag
      601 gccgctagag gacgacctac ggcagtgcga gtccgccggg gccgacctgg tctttgttcc
      661 gagcgtccca acggtttacc cgcacgggat aaattcgact ttcgtcgagg tgccaggtct
      721 ttccaacgtg ctcgaaggcg ccagccgccc tggacacttt cgcggcgtcg ccacggtggt
      781 gctcaagtta ttggaaatgg tccgtcccga cctggccgtc ttcggtcaaa aagattacca
      841 gcaacagctc ttggtcggac gcatgatcgc cgaccttcac gtacccgtgc aactcggtat
      901 ttgcccgacc gttcgagagc cggacgggct tgcgctctcc agccgcaacc gctatctcaa
      961 tccgcagcaa cgccaagcgg ctgtcgtgct caatcgcgcg ttggagcgcg cccaacaggc
     1021 cgtagacgcg ggagaacgcc gcggcgaccg aattcgacag attctccacg aaacactaaa
     1081 atcagaatca acggccgatc ttgactatgc cgaagtagca gacgccgaga cactagagcg
     1141 attgggagac ataggcaaat caacgcgtgc tgttgcgctg gtggccgccc ggtttggaac
     1201 gacgcggctg atcgataata ctctcctcac gacctgagcg actcgccggg ggaattcgat
     1261 ggggtatgcc gtcaattagg gaggatttcc aggcgcatgc gacaggtatt gttcacgata
     1321 ccgatcttcg gcgggctcaa ggtgttcggc tatggcgcct tgctcgtggt ggcgtttgtg
     1381 tcctccacat ggctggcgta ctggcgtgcc aagcgagagc ggctcgatcc cgaggtcatc
     1441 ggtgatatgt cgttctgggt attcctgttc ggtctgatgg gagcgcgtct cttttactgc
     1501 gtcgaatact ggggagacga catcaagagc ctctgggatg tcgtgcagta ctggaaaggg
     1561 ggcgtggtct actacggcgg aatcgtgggc ggagtcgctg ccttctttgt ctttcgagcc
     1621 ctccgaccgt ttcctttacg cccgtacatg gatgttcttg ccccctcgat cgcgattggt
     1681 acgttgtttg gccgcctcgg ttgttttatg aacggctgtt gtttcggtga catctgcagg
     1741 cttccctggg cggtttcgtt ccctgcctat tctcctccct gggtccagca cgtgaagtca
     1801 ggcctgattc ccgcgaatgc gctcaattct ctgccggtgc accccacgca gatctactcg
     1861 gcgatcgatg ccctggtgat cttcctgctg ttgacggcgt attacccact ccgccggcgc
     1921 gacggcgagg tcattggact gttgatgctg gtttatccaa tctcgcggtt cgcgatcgag
     1981 tatctgcgca acgacgaggg ggtcttctac ggcggaatga caatttccca gacgatcagt
     2041 gtgatattgc tgctattggc ctgcgtgtac tgggcctggc tatcaagttt gcccaaaggg
     2101 ctctatcgcg atctagccac gccgagcgaa tagccggggt cgttgccgct ggcgaccgcg
     2161 cgctcttgat caatcagggg acggggatgt ggtcgaggat gcgctgaagg atgtcgtcgg
     2221 ctgaaagccc ctcggcatcg gcctcgaagc tgaccagaat gcggtgtcgc atgatgtctc
     2281 gagccagact cttgacgtcg tgcggcgtga caaagtcgcg cccggcgagg agggcatgag
     2341 ctcgggcggc gcgcaccaag gcgatggttg cgcgtgggct ggcaccgagc tcgaggaggg
     2401 gtgccatcgc cagaccgaac gaagcaggat cgcgggtggc gcgcaccagg tcgacaatgt
     2461 attccttgat ggccggagca atccggatcg aggcggcaag agcccggaat tggagtacgt
     2521 cgtctggacc gtaaagcggc ggttcgtcac tggttcgcga atcgagatcg ggccggggtg
     2581 tgtcagacac gctcggcagg tcgagcatca agagttccga cgagcgatct gggtagccga
     2641 cgaccagctt catcaaaaac cggtcgagct gggcttccgg caaagggtag gttccctcgt
     2701 gctcgatcgg gttttgagtg gccagcaccc agaacgtgtc gggcaggaag atcgtttcgt
     2761 cgccgacggt gatctggccc tcctgcatcg cctcgagtaa cgcgctttgg actttggccg
     2821 gcgcccggtt gatctcatca gccaggagga cgctggtgac gatcgggccg ggccttacgt
     2881 cgaaggtgcc cgtcgccggc cggtagatct gtgttccggt caggtcggcc ggaagcaagt
     2941 cgggcgtaaa ctggatgcgg cggaacggca gatcaagggc ctgggcaagt gcacgcacgg
     3001 ccctggtctt ggcgaggcca gggaccccct cgataaggac gtgaccgcct gccagaagcg
     3061 caatcaccag ccgttccagg aggacccgct gtcccaccac caggtcttcc atccgctcga
     3121 tgagctgtct caaaccgcgc tgggccgcgg caatcgagtt gagggatggt tcgactcggt
     3181 caagatcgct cggcacggcg gcgttccatc aacctccgga gaatgtcact cgccgcatcg
     3241 cttgtatcct tgaactcatg cttggccggg ccggcctgtt ccttctgtgc ctttttggcg
     3301 gcgtagaacg ggttcgacgg gtcgaggaat tcttctggta catcgtcgac cacggtcgat
     3361 tcgtcctccg cgagagtttc gtcgtcttct tcgtcttcgt cgtcctcctg gtagaagggc
     3421 tcgtcctctt cggcttcgtc gtcaggctga cgctcatatt cgtcgtccga cgcagatggt
     3481 gccgttggag ggaccggtgc tgccacgggt ttgttggaag aggccgtgg
//
LOCUS       DATAQL010000925         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4827508, whole genome shotgun sequence.
ACCESSION   DATAQL010000925 DATAQL010000000
VERSION     DATAQL010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4827508"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VHS97_16100"
     CDS             complement(<1..907)
                     /locus_tag="VHS97_16100"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449779.1"
                     /translation="MIHHERLRPPSHDYPADEWNVIEKRFHPEFMAQSETMLALGNGY
                     LGMRGCPEEGGPNAENSTLINGFYEISSIVYPEDAYGFAKTGQTIFGVTDSKIIKLFV
                     DDEPFWLPNANLLSYDRRLNMKSGMLDREILWETPAGKQVLITSRRLVSFAERHVAAI
                     SYRVTLLNAAASLVISSEMAAHEPGALTDPNDPRQTKVFAGRVLHCRTSYAKARRIVL
                     CHATEKSRLTLTCATDHSLETSCPHTHKVAYTEDFGQVAFTIDARPGCPIDLTKYMVY
                     HTSQTASAEELCGRAEWTMDRVTTHG"
     gene            complement(940..1725)
                     /locus_tag="VHS97_16105"
     CDS             complement(940..1725)
                     /locus_tag="VHS97_16105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX3449780.1"
                     /translation="MSETNGKTPAQQPQTEWAITRDQYDAVLLDLDGVITDTASIHAA
                     CWKQMFDEYLQKRATQRGEAFRPFDPATDYRLHVDGKPRFDGVRDFLTSRGIQLPEGS
                     PNDPPQAETVGGLGNRKNDLVNGIIEDAGVEPYEGSVELIHQLRQRGFKLAVVTSSQN
                     CAAVLRAAKLDALFDIRVDGNTILAEHLAGKPAPDTFLMAAKLLGVEPSRAVVIEDAL
                     SGVEAGSKGNFGLVIGVARKGNAEELQQHGAHIVVDDLGELAD"
     gene            complement(1770..>2380)
                     /locus_tag="VHS97_16110"
     CDS             complement(1770..>2380)
                     /locus_tag="VHS97_16110"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3449781.1"
                     /translation="DRGRQRLTRLESKMNVFAIRHGETAWRLSGQHTGTTDIPLTGNG
                     RRLAERMRPVLAKEAIALVLCSPMQRARETCELAGLGDRAVIDPDLAEWNYGQYEGLT
                     PKQIHEGAPGWLIFRDGCPGGEIPEQVSARVDRVIARARAAVADVALFAHGHVLRVLA
                     VRWIGLPASAGQHFLLDTGTLCVLGYYRDIPAVKVWNGPLID"
BASE COUNT          430 a          733 c          703 g          514 t
ORIGIN      
        1 acccatgggt cgtaacccgg tccatagtcc actcggcgcg gccgcagagt tcttcggccg
       61 acgccgtctg cgacgtgtga taaaccatgt atttggtgag atcgatcgga cagccgggcc
      121 tggcgtcgat tgtgaaggcg acctggccaa aatcctcggt gtaggcaacc ttgtgggtgt
      181 gcgggcacga ggtctctaat gaatgatcag tcgcacacgt caaggtcaag cggctctttt
      241 cggtcgcatg acacagcacg atccggcggg ctttggcata gctggttcgg caatggagta
      301 ctcgacctgc aaagaccttc gtctgtcgcg gatcattcgg gtcggttagg gcgcccggtt
      361 catgcgctgc catttccgaa gagatcacaa gggaggccgc cgcgttgagt aatgtcacac
      421 ggtaggagat ggcggccaca tgcctttcgg cgaaagagac cagacgccgg gatgtgatca
      481 acacctgttt gccggccggc gtctcccaga ggatctcacg gtcgagcatc ccggatttca
      541 tgttgagccg ccgatcgtag ctcaagaggt ttgcattggg cagccaaaag ggctcgtcgt
      601 cgacgaagag cttgatgatc ttgctgtcgg tcacgccgaa gatggtctgg ccagtctttg
      661 cgaacccata agcatcttcc gggtacacga tcgagctgat ctcataaaac ccattgatta
      721 gagtgctgtt ctcggcgttg gggccgcctt cctccgggca cccgcgcata ccgagatatc
      781 cgttgccgag cgccagcatc gtttccgact gagccatgaa ttcgggatgg aaccgttttt
      841 cgataacgtt ccactcgtcg gccgggtagt cgtgggacgg cggcctgagg cgttcatgat
      901 ggatcatcgg gtggtccctc ccaatttagg gggcgatcgt cagtcagcca gttcgcccag
      961 atcgtccacc acaatatgcg ccccatgctg ctgaagctcc tcggcattgc ctttgcgcgc
     1021 gaccccgatc acaagcccaa agtttccctt agacccggcc tccacccctg aaagggcatc
     1081 ctcgatgaca accgctcgcg aaggctcgac cccgagtagc ttcgccgcca tcaagaacgt
     1141 gtccggagcg ggtttacccg ccaggtgttc cgctagtatc gtgttgccgt cgacccgtat
     1201 gtcgaacaac gcatccaact ttgcggccct taacaccgcc gcgcagttct ggctcgaagt
     1261 aacgacagcg agtttgaagc cgcgctggcg gagctgatga atgagttcaa cgcttccttc
     1321 gtaaggctcc acccctgcgt cttcaatgat cccgttgacc aggtcgttct tgcggttccc
     1381 gagcccgccc actgtttcag cctgtggcgg atcgttcggg cttccctcgg ggagttggat
     1441 gccgcgcgat gtgagaaagt cgcgaacgcc gtcgaagcga ggctttccat caacatggag
     1501 ccgataatct gtggcgggat caaaagggcg aaatgcttcg cctctctgcg ttgcacgctt
     1561 ctgaaggtat tcgtcgaaca tctgcttcca acacgccgcg tggatgctgg cggtatccgt
     1621 gatcacgcca tcgagatcga gcaggacagc atcgtattgg tcgcgtgtga ttgcccattc
     1681 ggtctggggc tgctgcgctg gtgtctttcc attagtttcg ctcatactgt cgtcctcccc
     1741 gtctctactt gtctgtcgat cgtcgccggc taatcaatga gaggcccgtt ccagaccttc
     1801 acggcgggta tgtcgcggta gtagccgaga acgcagaggg tgcctgtgtc gagcaggaaa
     1861 tgttgtccgg cgctggctgg caacccgatc cagcgcactg cgaggacgcg taagacgtgc
     1921 ccgtgggcga acagtgcgac gtccgcgacg gccgcgcggg cccgggctat tacccgatct
     1981 acccgcgcac tgacctgctc cggtatctcg ccgcctgggc agccgtctcg gaagattagc
     2041 caacccggcg caccctcgtg gatctgtttc ggtgtcagcc cctcgtattg gccgtaattc
     2101 cactcggcca agtcgggatc gataactgcc ctgtcgccga gacccgccaa ctcacacgtt
     2161 tcccgcgcgc gctgcatcgg gctgcagaga acgagcgcaa tcgcctcctt ggcgagcacc
     2221 ggccgcatcc gctcggcgag ccggcgccca ttgccggtca acggaatatc cgtcgtcccg
     2281 gtgtgctgcc cgctcagcct ccacgcggtc tcgccgtgtc tgattgcgaa gacgttcatt
     2341 ttcgactcca gccgcgtcag acgttgtcgg cctcggtcga
//
LOCUS       DATAQL010000926         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2996234, whole genome shotgun sequence.
ACCESSION   DATAQL010000926 DATAQL010000000
VERSION     DATAQL010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2996234"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1583)
                     /locus_tag="VHS97_16115"
     CDS             complement(57..1583)
                     /locus_tag="VHS97_16115"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3449782.1"
                     /translation="MSTTPVDRDLPGSVNMLIGGRWQASHSTRFGPVFNPSNGRIIAS
                     VPFCTTDDVDRAVRAAAAALPAWAETPVVERARVMFRFREKLAAHAEELAYLVTREHG
                     KTLAESRASVHRGVEVVEFACGIPSLIMGQSLANIARQVDCETLRHPVGVCAGITPFN
                     FPAMVPLWMFPIAIVCGNTFVLKPSEKVPLSAVRLGELLMESGLPDGVFNIVHGDRVC
                     VDALLSHPLVRAVSFVGSTAVARHVYITGTQNGKRVQAAGGAKNHMIIMPDADLDQTV
                     GALQLSAFGCAGERCMAGSIAVPVGKVADSLVERLCKTAQSMKVGATDGGAEVDMGPV
                     ITREHLDRVAGYVDIAKREGADVVLDGRSATPKNGAFLIGPSVLDRVEPSMRVAKEEI
                     FGPVLSVIRALDLEHALAIGRDCPYGNGASIFTRSGWAARQFKQHFNAGMIGINVGVP
                     APMAWFPFTGWNSSFFGDLHIQVTESIHFYTQQKMTMTRWFESPQESHHDPVWKTGKG
                     "
     gene            1754..2623
                     /locus_tag="VHS97_16120"
     CDS             1754..2623
                     /locus_tag="VHS97_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX3449783.1"
                     /translation="MSRVVRGALIQARLCEPATSPAAVVKKAMIDKHIALIASAAKEG
                     AQVACLQELFYGPYFCAEQQARWYELTERVPDGPTTKLMAELARQHQIVLVVPVYEED
                     LTGVYYNTAAVIDADGRYLGKFRKMHIPHCAPGFWEKFYFRPGNLGYPVFETRVGKVG
                     VYICYDRHFPEGARCLGLNGAEIVFNPSATVAGLSEYLWKLEQPAHAVANGYFVGAIN
                     RPGFEEPWRIGEFYGQSYFCDPRGQMLAVGKRDNDDIVIADLDLDLIREVRNTWQFFR
                     DRRPETYGSIIEL"
BASE COUNT          525 a          822 c          809 g          495 t
ORIGIN      
        1 gagatcccgg atctgattgt tcccgcggcg aaagctacga aataaagctc gccgtctcag
       61 cccttccccg tcttccaaac cggatcatga tgcgattcct gcggagattc gaaccagcga
      121 gtcatggtca tcttttgctg agtatagaaa tggatcgatt cggtcacctg gatgtgcaag
      181 tcgccgaaga acgaactatt ccaccccgtg aacggaaacc atgccatcgg ggcagggacg
      241 cccacgttga tgccgatcat ccctgcgttg aagtgctgct tgaactgccg ggccgcccag
      301 ccgctgcgtg tgaagatcga ggcaccgttg ccatacgggc aatcgcggcc gatcgccagc
      361 gcgtgctcga ggtcaagggc gcggatcacc gagagcacag gtccgaaaat ctcttccttc
      421 gcgactctca tcgacggctc gacccggtcg agcacgctgg ggccgatcag gaaggcgccg
      481 ttcttgggcg tggccgagcg gccgtcgagg acgacatcgg ccccttcgcg tttggcgatg
      541 tcgacgtagc cggccacccg gtcgaggtgc tcgcgagtga tgaccggccc catgtcgact
      601 tcggcaccgc cgtcggtcgc accgactttc atagattgcg cggtcttgca aagccgttcc
      661 acaagcgaat cagcaacctt gccgaccggc accgcgatgc tgccggccat acagcgctcg
      721 cccgcacagc cgaaagctga gagctgaaga gcgcccactg tctgatcgag atcggcatca
      781 ggcatgatga tcatatgatt cttggcgccg ccggccgcct ggacccgctt gccgttttgc
      841 gtacctgtga tgtaaacgtg gcgagcgacc gccgtcgaac ccacgaatga gaccgcgcgc
      901 accaaggggt gagacagaag ggcatcgacg cacacgcggt caccgtgcac gatattgaat
      961 accccgtccg gcaatcccga ctccatcaaa agttcaccca ggcggaccgc cgacagtggg
     1021 accttttccg agggcttcaa gacgaatgtg ttgccgcaga cgatcgcgat cggaaacatc
     1081 cagagtggaa ccatggccgg gaagttgaac ggagtgatgc ccgcacacac gccgacgggg
     1141 tggcggagcg tctcacagtc gacctgccgg gcaatgttag cgagcgactg gcccatgatc
     1201 aggctgggaa ttccgcaggc gaactcgacg acctcgactc cgcggtgcac cgacgcgcgc
     1261 gactcggcga gcgtcttgcc gtgctcgcga gtgaccaagt atgccagctc ctccgcgtgc
     1321 gcggccagct tttcacgaaa ccggaacatg acgcgcgccc gttcgacgac cggtgtctcg
     1381 gcccaggcag gtagtgccgc cgcggccgct cgcacggccc gatcgacgtc gtcggtcgtg
     1441 caaaacggca cgctggcgat gatgcggccg ttcgaagggt tgaaaaccgg tccaaaccgg
     1501 gtcgaatggc tcgcctgcca ccggcctcca atcagcatgt tcaccgatcc gggtagatcg
     1561 cgatcgactg gagttgtgct catcttcgcg cctccgtgga aataggttcg accggcatct
     1621 taacataggg aggggccgca tgtaggccca agccgacgcc aacactcgtg gcccgctcgt
     1681 ccacactggc attgggtgct cgatcgatgt aggatcgtgc acatgtcgaa ggtcctaaac
     1741 tcgaggagtt gccatgtcgc gcgtcgttcg aggcgccttg attcaggcca ggctctgcga
     1801 gccggcgacg tcgcccgccg cggtcgtcaa gaaggcgatg attgacaagc acatcgcgct
     1861 catcgcctcg gccgcgaaag aaggggcgca agtcgcctgc ttgcaagagc tgttttacgg
     1921 tccttacttt tgtgccgagc agcaagcgcg gtggtatgag ctgactgagc gtgtacccga
     1981 cggaccgacg acgaagctga tggccgaact tgccaggcag caccaaatcg tgctcgtcgt
     2041 gccggtctac gaagaggatc tcacgggagt ttactacaat acggccgcag taatcgacgc
     2101 cgacggccgc tatctcggca aatttcgcaa gatgcacatc ccgcactgtg cacccgggtt
     2161 ctgggagaag ttttattttc ggcccggcaa cctgggatat ccggttttcg agacgcgtgt
     2221 gggaaaggtc ggagtctaca tctgctacga ccgccatttt ccggaagggg cccgctgcct
     2281 gggtttgaac ggtgccgaga tcgtgttcaa tccctcggcg actgtggccg gtctctcgga
     2341 gtacctttgg aagctcgagc agcctgccca tgccgtcgcc aatgggtatt tcgtcggcgc
     2401 gatcaatcgc cctggattcg aagagccgtg gcggatcggc gagttctacg gacagagcta
     2461 tttctgcgat cctcgcggac agatgctcgc cgtcggcaaa cgcgacaacg acgacatcgt
     2521 gatcgccgac ctcgatctgg acctgatccg agaggtccgt aatacatggc agtttttccg
     2581 cgatcgtagg cccgagacct atgggtcgat cattgagctc taagccgcac ccacgtcgcg
     2641 gcccagcctc c
//
LOCUS       DATAQL010000927         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3147704, whole genome shotgun sequence.
ACCESSION   DATAQL010000927 DATAQL010000000
VERSION     DATAQL010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147704"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VHS97_16125"
     CDS             <1..1101
                     /locus_tag="VHS97_16125"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX3449784.1"
                     /translation="QAAIEAGATTVNIPDTVGYATPTQYAGVIKTLRDRVPNIEQAII
                     STHCHDDLGLAVANSLAGCEAGARQVECTINGIGERAGNAALEEIVMALKTRFDYYGL
                     TTAIKTERLYPSSRMLSTLTGLAVQRNKAIVGRNAFAHESGIHQDGMLKDRSTYEIMR
                     PEDVGVPRTDLVLGKHSGRHALRERVAEMGYTLSEEQLETLFHDFKALADKKKEVYDD
                     DLVVLIEKYLENVPAHWQLVSLHTTAGLSILPTATIAIRRPDGEIVQDAAIGDGPVDA
                     IFKAVERVTGVRANLHEFVVRSVTQGEDAQGEVTLELEVESGDRSFRGRAASTDIIEA
                     SAQAYLNAVNAIVTHKDRGHVREVIGRPGAGA"
     gene            complement(1140..1712)
                     /locus_tag="VHS97_16130"
     CDS             complement(1140..1712)
                     /locus_tag="VHS97_16130"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX3449785.1"
                     /translation="MTTYNLDEYYPISPLDPRSYRAYMHRYVFSQVDLPAHHAHVLDG
                     TVPEAFVAAHAADFDRWIEADGGLDVQLLGIGRNGHIAFNEPTSLSIEEALRLPTRLV
                     ALHPVTRADAAKEFGGEDLVIPRALTMGVASILAARSIVILATGTRKAEIVARAITGP
                     MTAELPASLLQSVAEKVTWVVDEEAARGLT"
     gene            complement(1709..1933)
                     /locus_tag="VHS97_16135"
     CDS             complement(1709..1933)
                     /locus_tag="VHS97_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449786.1"
                     /translation="MDAAPRDYAGMKILVNPDAELASRAAADLLIKAIDNARAIRGRA
                     VAGLATGTTPERVYSRLVERHRLGCSHFVM"
     gene            complement(1960..>2892)
                     /locus_tag="VHS97_16140"
     CDS             complement(1960..>2892)
                     /locus_tag="VHS97_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3449787.1"
                     /translation="LLDTVRGCGPHGQLVDVRDSLRSVLSPSRPLVMLCPHADDGAIT
                     AACLLHEYAVRRGLPVIEVLLFAGERNVAAPWLNTEKKVTVREAEFQLESNVLGAEGI
                     CWDLEAYRMPGYEPTALDISKVVEWFGARRPGAVIVPPANDAHMAHRATRALAAIGLV
                     GAKLTDCLVLTGWTPWGPLAEPNAYFPYDGEAERTKEWAIHCHASQVLLTDYTQYCSH
                     LGRAYAALVREWAEGHSLAGRAHRTEDRFVGVELFQIDTYDPRKAKSYPPDPIQIALG
                     ILSGQVATEASDPVGAASAVIAGSSSKAAVVAHL"
BASE COUNT          574 a          892 c          936 g          490 t
ORIGIN      
        1 caggccgcca tcgaggctgg cgccaccacc gtgaacatcc ccgacacggt cggctacgcc
       61 acgcccacgc agtatgccgg cgtgatcaag actctccgcg accgcgtccc caacatcgag
      121 caagcgatca tcagcaccca ctgccacgac gaccttggat tggcggtcgc caacagcctg
      181 gccgggtgtg aagccggagc gcgacaggtc gagtgcacca tcaatggcat cggcgaacgt
      241 gccggcaatg ccgcgctcga agagatcgtg atggccttga agacccggtt cgattactac
      301 gggctgacga cggccatcaa gaccgagcga ctctatccgt cgagccggat gctctcgacg
      361 ctcacgggac tggccgtgca gcgcaacaag gcaatcgtgg gccgcaacgc attcgcacac
      421 gagtccggca tccaccagga cggcatgctc aaggatcgct cgacctacga gatcatgcgc
      481 ccggaggacg tcggcgtgcc ccgtaccgat ctggtactgg ggaagcactc cggccggcat
      541 gccttgcgcg agcgcgtcgc cgagatgggc tacacgctga gcgaagaaca gctcgagacc
      601 ttgtttcacg acttcaaggc cctggcggac aagaagaagg aagtttacga cgacgacctg
      661 gtcgtactga tcgaaaagta cctggaaaac gtgccggcgc actggcaact ggtcagtttg
      721 cacacaacgg cgggcctttc tatcttaccc acagccacga tcgctatccg tcggccggac
      781 ggcgaaatcg tgcaggatgc cgccattggc gacggaccgg tcgacgcgat tttcaaggcc
      841 gtcgagaggg ttaccggcgt gcgggccaat ctccacgagt ttgtggttcg aagcgtgacg
      901 cagggagaag acgcacaagg ggaggtgacc ctcgagctcg aggtcgagag cggcgatcgc
      961 tcattccgcg gtcgagccgc gtcgactgac atcatcgagg cctcggcgca ggcctacttg
     1021 aacgcggtca acgccatcgt cactcataaa gaccgcggcc acgtgcggga ggtgatcggc
     1081 cggccgggcg caggggcgtg atagaaagcc tgcagcgcca tatcgccggt ccagaggaat
     1141 caagttagac cccgcgccgc ctcttcgtcg acgacccagg tgaccttttc ggcgaccgat
     1201 tggagtagcg aggctggtag ctcggcagtc atcggaccag tgatggctcg ggcgacgatc
     1261 tcggccttcc gcgtgccggt ggcgaggatc acgatcgacc tggccgccag gatcgatgcg
     1321 acacccatgg tcagcgcgcg ggggattacc agatcctcgc cgccgaattc tttagccgcg
     1381 tcggcgcgcg tgacggggtg aagcgcgacg agacgggtcg gcaggcgaag ggcttcttcg
     1441 atgctcaaac tggtcggctc gttgaatgcg atatggccgt tgcgtccgat tccgagaagc
     1501 tgcacgtcca gcccgccgtc ggcctcgatc caacggtcga agtccgccgc atgcgcggct
     1561 acaaatgctt cgggaaccgt gccatcgagg acgtgcgcat gatgcgctgg gagatccacc
     1621 tggctgaaca cgtaacggtg catatacgca cggtagctcc tgggatcgag cgggctgatc
     1681 gggtaatact cgtcgaggtt ataggtcgtc acatcacgaa atgagagcag cccaggcggt
     1741 ggcgctcgac gaggcgggaa taaacccttt caggcgtagt gccggttgcc agccccgcca
     1801 cggcccggcc gcgaattgcg cgcgcgttgt cgatggcctt gatgaggaga tcggcggctg
     1861 cacggctggc aagctctgcg tcgggattga ccagaatctt catcccggca tagtctcggg
     1921 gcgctgcgtc catcactgcg agaatccttg ggcaagctct cagagatgcg cgactactgc
     1981 ggctttcgag gatgatccgg cgatcaccgc agaagcggca ccaaccgggt ctgacgcttc
     2041 ggtcgcgacc tggccgctca agattcccag cgcgatctgg atcggatcgg gggggtagct
     2101 cttggccttt ctggggtcat aagtgtcaat ctgaaagagc tcgacaccta cgaagcgatc
     2161 ttcggttcga tgagcgcggc cggccaggct atggccctcg gcccactcgc gcacgagcgc
     2221 ggcgtaggct cggcccaggt gagagcaata ctgggtatag tcggtcaaga gaacctgcga
     2281 ggcatggcaa tggatggccc attccttggt gcgctcggcc tcgccatcgt aggggaagta
     2341 ggcgttcggc tccgcaagcg ggccccatgg agtccagccg gtaagtacca agcaatcagt
     2401 cagcttggca ccaaccaatc caatcgccgc cagagcgcgg gtagcccggt gcgccatgtg
     2461 ggcatcgttg gcaggcggga cgatcacggc gccgggtctg cgggcaccga accactcgac
     2521 gactttgctg atgtcgagcg ccgttggctc gtagccgggc atacggtagg cctctaagtc
     2581 ccaacagatt ccttcggcac ccagcacgtt gctttcaagc tgaaactcag cttcgcgaac
     2641 cgtcactttc ttttcggtgt tgagccacgg agcagccacg tttcgttcgc cggcgaacag
     2701 cagaacctcg atcaccggca gcccacggcg aaccgcgtat tcgtgaagca agcacgccgc
     2761 agtgatcgct ccgtcgtctg cgtgggggca aagcatgacc aagggccgag acgggctcag
     2821 gactgatcgg agggagtcgc ggacgtccac gagctggccg tgcggaccgc agcctcgcac
     2881 ggtatcaagc aa
//
LOCUS       DATAQL010000928         9096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3607991, whole genome shotgun sequence.
ACCESSION   DATAQL010000928 DATAQL010000000
VERSION     DATAQL010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3607991"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..934
                     /locus_tag="VHS97_16145"
     CDS             362..934
                     /locus_tag="VHS97_16145"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEX3449788.1"
                     /translation="MRFVLIDRIVDVQPGRTRLAGSATFSTPGPSLVAVKNLSLAEEY
                     LSDHFPGFPVMPGVLMLEALTQAGAWLIRDMEDFASSIIVLKQAKTIKYGSFVEPGRQ
                     LELRVEMTSHDPVDSSFKGVGVIDGQEMVKGRFTLTRYNLRDRSPELHTTDSAIVSGL
                     RDLYTTLRKGSVGARAIARRPEVHELPTPV"
     gene            1338..1724
                     /locus_tag="VHS97_16150"
     CDS             1338..1724
                     /locus_tag="VHS97_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3449789.1"
                     /translation="MPTYDEIFEKVQATLVDALGVDEEEVTREATLQGDLGAESIDFL
                     DIVFRLERNFGIKIPRGELFPENLMADPEWVADGKLTSKGVGELKARLPFADLSKLED
                     NPAVDRIGDLYTVEMLVQYVHSKLAA"
     gene            1894..2430
                     /locus_tag="VHS97_16155"
     CDS             1894..2430
                     /locus_tag="VHS97_16155"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEX3449790.1"
                     /translation="MRWIWIDKFLEFRSGQFARAIKNLTLAEEHLHDHFPGYPVMPAS
                     LIIEGLAQTGGILVGEAGGFAEKVVLAKIPRAEFHGVACAGDQLIYEVTLTDLRSEGA
                     VVAAKAFLDGELLAEAEIVFAHLDNSRANQIFGPKNFVFTQQLLGVLDLAKAQQRARD
                     ADLSAEAPADSNGKPHDS"
     gene            2655..3974
                     /locus_tag="VHS97_16160"
     CDS             2655..3974
                     /locus_tag="VHS97_16160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX3449791.1"
                     /translation="MLGSERRVAITGLGLVTPLGDSPDRVWTSLVEGRGMVRPIEAFR
                     VAGLPSDAGGEIRDFDLKKYSLPTFWSALRKTKKYLARDIQLAVAAAQLAVVDAGLLG
                     GVVDPTRMGIDLGAGLISSELDELAPAIAHAYGSSNTFDFQVWGRESIEMIEPIWLLK
                     YLPNMLACHISILIDCQGPSNTITEADASSNLAIAEAARIIARGRADVMITGGADSKI
                     HPLSLVRMALFETMSRSKGDPSQACRPFDRRRDGWVPGEGAGIVILEEWEHARKRGAR
                     IVGEVLGGGSGCDAMPSGGLDPEGAGTEVAITAAIEEAGLKPADVGHVNAHGSAGRVS
                     DLAEARAFRRIFGPGQIPVTALKGYMGTLASGCGAVEMAASLIGVNRGGIPAILNCEQ
                     PDAEIELDLDLVLKTPRPTQNPVFVTTNLTPNGQAAALVIRGCPIEP"
     gene            4043..5329
                     /gene="fabF"
                     /locus_tag="VHS97_16165"
     CDS             4043..5329
                     /gene="fabF"
                     /locus_tag="VHS97_16165"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX3449792.1"
                     /translation="MRRVVVTGMGMVTPIGGDLESTWSALLSGKSGVGPITLFDARSL
                     PTRIAAEVTSFRLEDYIDRSERWADHSRNSKFALAAASMAIKDSGLLESKPELNRRRF
                     GVYLGSGEGQQDFPRFVHLVHRSTRDGRVDTSEFTRLGVKELNLIREAEQEPGTPSGH
                     LASVFGARGPNANCLTACAASSQAVGEAFELIRRGNADVILSGGTHSMIHPFGVTGFI
                     RLTALSTRNDEPQRASRPFDRDRDGFILGEGAGMLVLEELEHAKARGGKIHGEIVGYG
                     SSADAFRITDSHDQGRGAIACMREALEVARLGPDDIDYINAHGTSTSVNDSIETLAIK
                     RSFGEAAYNVPISSTKSMMGHLIAAAGSVEAIVCLLTIRDGMLPPTINLENPDPECDL
                     DYIPNEARRKQVDVALSNSFGFGGQNITLIIKRFVD"
     gene            5432..6517
                     /locus_tag="VHS97_16170"
     CDS             5432..6517
                     /locus_tag="VHS97_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3449793.1"
                     /translation="MIGLSSFSSVLLLLLAIPTLLGLAGVVYLTVKYTPVIIRIFQAQ
                     PLFMPLKVTPVERGESVDFQTTDGLRLCGSYLRARTDEQAGVIVYCHEYLSDRWSFCP
                     YVDHLRDLGYDIFTFDFRNHGASDLDRTYAPLQWTTEHEIDDLRAALSFLRTRDDHDP
                     AGFGLFGVSRGGTTALITAATEPDVWGVVTDGAFPTQGTMVSYIVRWAEIYVPSEFLR
                     SLIPMWLYGFLARTARLCTERSLRCRFPSVEAAVSRLTPRPWLMIHGQRDTYIGPEIA
                     QALFDRGEDPKELWLVPDAKHNRCRETAPEAYTARLQDYLARFAPRRPLNETPEVISS
                     AHRVISSDFAHQLAPALSREVAAPIPG"
     gene            6545..8236
                     /locus_tag="VHS97_16175"
     CDS             6545..8236
                     /locus_tag="VHS97_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX3449794.1"
                     /translation="MIKMLMRMIGRPVARRARRLAYTFLDQTQHAADVQRDLLLRRLA
                     RHADSQFGRDHHFHEIRTPADFRKRVPIGGYDRHEPYIDRVRHGDTNALFGDGTQVLM
                     FAMTSGTTNRPKTIPVTTEALTDYREGWTIWGILAFDAHREMMRDGLKPILQVASDWQ
                     ESFTSSGIPCGAITGLTAHMQNRFVRTKYCMPAIASRIKDIESKYYLGLRFSIYRDLG
                     TIIAANPSTILAMARLGDRQKEVLIRDLADGTIDARWEIPADIRRHLSRKTRRRHKAV
                     ARAFEKIANDTGRLLPKDYWPNLQFLSNWMGGTMRAYLRGYPEFFGETPVRDVGLIAS
                     EGRMTIPIEDGTPAGILDVRHHYFEFIPEEEGDRELPDTVEAADLIEGRNYFIVLTTA
                     GGLYRYNISDLVRCVGFHGQAPLIEFLNKGAHYSSLTGEKLSEHQVIAAVEAAQRAAG
                     VHLRSYLLVPTWDEPPFYSLLVEESDLVSSASHERLAVAVERELRSQNVEYVSKRDTL
                     RLGPVRILRIADGSWAEFQKRRLVKSGGTVEQYKQPHLVPDLDLITSFRQLEVVS"
     gene            8484..>9096
                     /locus_tag="VHS97_16180"
     CDS             8484..>9096
                     /locus_tag="VHS97_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449795.1"
                     /translation="MNTARDCVRSLSPWMVACLGLGLCLVDAGCSGISGLRSVGTERP
                     SLLSFWDHQGPGSPTPDNDAYAQAMRPSRARAEAIAKRSDSPAVDGEEGSTTRDGIEL
                     AEAAEPIRSSQHPHATNSADPSVRVSLGAPEPLPGVALADSAENRSQKVAATGRAPSW
                     KREKTDSSLASGLPLSQPADGDDVKNQAPSVITEREQAAGPAET"
BASE COUNT         1799 a         2656 c         2781 g         1860 t
ORIGIN      
        1 tggccatctc ctgggcacga gcgtactttg gccgagtgaa attgtgcccg cgcggcatga
       61 tggggggcac gaggggagac gaggggccgt tgcctcaacc gattgaggtg gcacgcttcg
      121 atcaagtcag agaattctca ccttgcccag gttgacggga cgcaggtgga ttgtatgttt
      181 gctgcaagac gttatgtcaa aaaaccggct gatgcgactc ccttggcctc ttgaacctca
      241 cggcgacgtc ctgctagatt actgagccaa cagtacgaca cccatgaggc tgggtcgctt
      301 tgtacccagt tctggcagct cttactttaa acgggttaac aaaccgactt cggaaaaatc
      361 catgcgcttc gtcttgatcg accggatcgt tgatgttcag cccggacgga cacggttagc
      421 cgggagcgct acattttcca ctccaggacc gtcgctggtc gcggtcaaaa acctctcgct
      481 ggccgaagag tatctgagcg atcactttcc gggctttccc gtgatgccgg gagttttgat
      541 gctggaagcg ctgacgcagg cgggtgcctg gctgatccgg gacatggaag actttgcgag
      601 cagcatcatc gttttgaagc aagccaagac aatcaaatat ggcagcttcg tggaacctgg
      661 ccgtcagctc gagctgcgtg tcgagatgac ctcgcacgac cccgtggaca gctcgttcaa
      721 gggggttgga gtgatcgacg ggcaggagat ggtcaaagga cgttttacat tgacccgtta
      781 taacttacga gaccgaagtc ccgagctgca cacaaccgac tctgcgatcg tctcgggcct
      841 ccgcgacctc tatacaaccc tccgcaaagg atcagtgggt gcccgggcga tcgcacgacg
      901 gcccgaagtg cacgaattgc ccacgcccgt ctaggccggt tgtcagttcg tcgtttcgat
      961 tctggcatcg gcccccctat tgtcccccgc actctttctc tagtcggcaa aatccggatt
     1021 tccagaattc caggattttc ccggcacgtc aacttgcgtt cccatttcgg gaagatattg
     1081 ggaattttgc gtgttacctg atgatgttgc aacgatttgc ttttttgcac atgatatgac
     1141 tcgaatttcg acccctcgat tgccgcaagg ccgtccgggg gtcgtggcat gcgacgaaaa
     1201 tccccattgt tcgtccattt gagtttggcg cgcgatttcg ccgacgatta agaaaggcgg
     1261 ctttatcggc ggcgcatggc tcgcccgttt ccggtttggg attggagggg caatcccatt
     1321 ttgatcctga ggataaaatg ccaacttacg acgagatctt cgagaaagtc caggccacgt
     1381 tggtggatgc cttgggcgtg gacgaggaag aagtgacccg tgaggccact cttcagggtg
     1441 acctgggcgc cgagtcgatc gactttctgg atatcgtatt tcgcctggaa agaaatttcg
     1501 ggatcaagat cccacgcggc gagctgtttc cggaaaactt gatggccgac cccgagtggg
     1561 ttgccgacgg caagcttacg tccaaggggg tgggagagct caaagcccgg cttccgttcg
     1621 ctgacttgtc caagctcgaa gacaatcccg ccgtcgaccg gatcggtgat ctttacacgg
     1681 tcgagatgct cgttcaatat gtccacagca agctggccgc ctgagcgatg cttgcagtcg
     1741 tgcatttgtt gcgttcgggc ctgagcgtgg ttgagacgct caggcatgcg agcggctgcc
     1801 ggagcgtcga gaggggatcg cctctcgact ttgagttttg atcgatttgg gctcgccagc
     1861 gaccgttggt tctttcgtcg ggtgctgcag gcaatgcgat ggatctggat cgacaaattc
     1921 cttgaatttc ggagcgggca gtttgcccgt gccatcaaaa acctcaccct ggcggaagaa
     1981 catctccacg atcatttccc gggctatccg gtgatgccgg cgtcgctgat catcgagggg
     2041 ttggcgcaaa ccgggggaat tttggttggc gaggccggtg ggtttgccga gaaagttgtc
     2101 ctcgccaaga ttccccgcgc agagtttcat ggagtggcgt gcgcggggga ccagcttatc
     2161 tacgaggtca cgctgacgga tttacggagt gaaggggctg tcgtcgccgc caaggcattc
     2221 ttagacggtg aattgctcgc cgaagccgag atcgtgtttg cccaccttga caattcccgc
     2281 gccaatcaaa tctttggccc caagaatttt gtgtttactc aacaattgct gggagtgctc
     2341 gacctcgcca aggcacaaca gagggcacgg gacgcggact tgtctgccga agcgccggcc
     2401 gactccaacg gcaagcccca cgattcgtag tcgatcaatt ggtcccacca ctccgcgatg
     2461 gatgggcgtc agagccgcga gagtttgccg gaacggaatt ccaggatgtc cgcgaaaaat
     2521 ttgcgtgttg cgtcaagttt atgccgccgg gattgctccg ggaagcaata atgttgaagt
     2581 agttcgagtg gaacggaccc ttctctagct ggcgccctat acgacccgcg acgccgtaat
     2641 tcgacgagtg attcatgctt ggttccgaac gccgcgttgc aataaccggt ctgggcctgg
     2701 tgacaccgct gggcgatagt ccggatcgcg tttggacttc ccttgtcgaa ggtcggggaa
     2761 tggttcgccc catcgaggcg tttcgagtgg cgggtcttcc gagcgacgcg gggggcgaga
     2821 ttcgagattt cgatctcaag aaatactcgc tgccgacgtt ctggagcgct ctccgtaaga
     2881 cgaagaagta tttggcgcgg gacatccagc ttgcggtggc ggcggcacaa ctggccgtgg
     2941 tcgacgccgg gctgttgggg ggcgtcgtcg accccacacg catgggaatc gacctggggg
     3001 ccggcctgat ctcgagcgag ctcgacgagc tggcgccggc aattgctcac gcatacggca
     3061 gcagcaatac tttcgacttt caggtctggg gccgagaatc gattgagatg atcgagccga
     3121 tatggctctt gaaatacctg cccaacatgc tggcctgcca catctcgatt ttgatcgact
     3181 gccagggccc gagcaatacg atcaccgagg cggacgcgtc gtcgaatctg gcgatcgccg
     3241 aggcggcgcg gatcattgcc cgcggacgag ccgacgtgat gatcaccggt ggtgcggatt
     3301 ccaagattca tcccttgagc ctggtacgta tggcgctctt tgagaccatg tcgcggtcca
     3361 agggtgaccc gagccaggcg tgccgtccgt tcgatcgccg ccgcgacggc tgggtgcctg
     3421 gcgagggggc tggcatcgtg attctcgagg aatgggagca tgcccggaag cggggggcgc
     3481 gcatcgtggg agaagtgctg ggtgggggct cgggttgcga tgccatgcct tccggcggcc
     3541 ttgaccctga gggagccgga actgaggttg ccatcaccgc ggcgatcgag gaagcgggac
     3601 tgaagcccgc cgacgtcggc cacgtcaatg ctcacggatc ggccgggcgg gtttccgacc
     3661 tggccgaagc aagagcgttt cgccgaatct ttggaccggg ccagatacca gtcacggcct
     3721 tgaagggtta catgggaacc ctggcatccg gttgcggcgc cgtcgagatg gccgccagct
     3781 tgattggcgt gaatcggggc gggatcccgg ccatactcaa ctgcgagcaa cccgacgcgg
     3841 agatcgagct cgacctcgac ctggtgctaa agactccgcg gccgacccaa aatccagtat
     3901 ttgtcaccac caacttgaca cccaacggcc aggcggcggc cctggtcatc cgcggttgcc
     3961 cgatcgaacc ctagcgcaaa gccccggcca gggccgcggt agaccgcggc ttgtgctcgg
     4021 agcgtaatgc gaggaaggct gtatgcgtcg cgtcgtcgta acgggcatgg gaatggttac
     4081 accgatcggg ggcgacctcg agtcgacctg gtcggcgctt ttatccggca agagcggtgt
     4141 cgggccgatc acgcttttcg acgcacgctc cctgccgact cgcatcgcag cggaggtgac
     4201 cagtttccgg ctcgaagact acatcgatcg gtcggagcgt tgggccgatc attcgcgcaa
     4261 tagcaagttc gccctggcgg cggcgtcgat ggcgatcaaa gactcaggcc tcctggagag
     4321 caagccagag cttaaccgaa ggcggtttgg cgtttacttg ggatcgggag aaggccagca
     4381 agattttccc aggttcgtgc acctggttca ccgttccaca cgcgacggcc gtgtcgacac
     4441 gtcggaattc acccggctcg gcgtgaaaga gctgaacctg atacgcgaag ccgagcagga
     4501 gcccggaact ccctccggcc atcttgcgag cgttttcgga gccaggggac ccaatgccaa
     4561 ctgcttgaca gcatgcgccg ccagctcgca ggcggtgggt gaggcgttcg agctgatccg
     4621 ccgcggcaac gccgatgtca tcctttcagg cggcacgcac agcatgatcc atcccttcgg
     4681 cgtaacagga ttcatccgct tgaccgcgct atcgacgcgc aacgacgagc cccagcgagc
     4741 cagccgaccc tttgaccgcg accgcgacgg ctttatcttg ggcgaagggg ccgggatgct
     4801 cgtactcgaa gagcttgagc atgccaaggc ccgcgggggt aagatccacg gcgaaatcgt
     4861 cggatacggg tcgagtgccg atgcgtttcg aattaccgac agccacgacc aaggcagggg
     4921 agccatcgcc tgcatgcgcg aggccctgga agtcgcgcgg ttgggtcccg acgacatcga
     4981 ctacataaac gcacatggca ccagcaccag cgtcaatgat tcgatcgaaa ccctcgccat
     5041 caagcgatct ttcggcgagg cggcatacaa tgtgccgatt tcgagcacca agagcatgat
     5101 gggccattta atcgccgcag ccggcagtgt cgaggcgatt gtttgcctcc tgaccattcg
     5161 ggatggtatg cttcccccaa ccatcaatct tgaaaaccca gaccctgagt gcgacctgga
     5221 ttacattccc aatgaagcgc ggcgcaagca ggtagacgtg gctctttcaa atagctttgg
     5281 ttttggtgga cagaacatca cacttattat caaacgcttc gttgactaac cgatcgtcat
     5341 cgccgacccc gcccctggcg gccgaccctc gcgctaacac ggttaccgcg cgccgagctc
     5401 accccacagc gacacgatca ggagtgcatc tgtgattggc ctatcgagtt tctcttccgt
     5461 tcttctcttg ctactcgcga taccgactct gcttgggctc gcgggcgtgg tttatctcac
     5521 ggtcaagtac acgcccgtga tcatccgcat ttttcaagcg cagcccctct tcatgccgct
     5581 caaggttacg ccggtcgaac ggggagagtc ggtcgatttt cagaccactg acggactgcg
     5641 tctctgcggc agttatttgc gagcacgtac cgacgaacag gccggcgtga tcgtttactg
     5701 tcatgaatac ttgagcgatc gctggagttt ttgtccctac gtcgaccatc tccgtgatct
     5761 cggctacgac atcttcacgt ttgattttcg caatcatgga gccagcgatc tcgatcgcac
     5821 ctacgcgcct ttgcaatgga ccaccgagca tgagatcgac gacttacgcg ccgcgctctc
     5881 ttttctgcgt acgcgtgacg atcatgatcc ggcgggtttc gggctttttg gtgtcagccg
     5941 agggggaacg acggccctga ttacggccgc caccgaaccc gatgtgtggg gggtggtcac
     6001 cgatggcgcg tttcccaccc agggcacgat ggtctcatat atcgtccgct gggcagagat
     6061 ttacgtcccc agcgaatttc tgcgaagtct cataccgatg tggctttacg gattcctggc
     6121 caggacagcc cgtctctgca cggagcggtc gctgcgctgc cgttttccca gcgtcgaggc
     6181 ggccgtttcc cggctgacgc ctcggccctg gctgatgatt cacggccagc gcgacacgta
     6241 tatcggtccg gagatcgcgc aggcgctctt cgatcgcggc gaagatccca aggaattgtg
     6301 gctggttcct gacgccaagc acaaccgctg ccgcgaaacc gctcccgagg cctacacggc
     6361 gcgactccag gattatctgg cacgattcgc tcctcgccgt ccactgaacg aaacgcccga
     6421 ggttatctcg tcggcgcacc gggtgatctc gagcgacttt gcccaccaac tggcgccggc
     6481 tctctcgcga gaggtggcgg ctcccatccc gggttgacca agtgagtcgg ggaattggcc
     6541 ggccatgatc aagatgctca tgcggatgat cggtcggcct gtcgcccggc gtgcgcgccg
     6601 gctggcctac acgtttctcg accaaaccca acatgccgcc gacgtgcaac gcgacctgct
     6661 cttacgacgg ctggcccggc atgccgacag ccaatttggt cgcgaccatc actttcatga
     6721 gattcgcacg ccggcagatt ttcgcaaacg cgttccgatc ggtggatacg atcggcacga
     6781 gccctatatc gatcgcgtcc gccacgggga tacgaacgcc ctcttcggcg atgggacgca
     6841 ggtcttgatg tttgcgatga cctcgggcac caccaaccgc cccaagacga tcccggtcac
     6901 caccgaagca ctgactgact accgcgaagg gtggacgatc tgggggatcc tggcattcga
     6961 cgcccatcgc gaaatgatgc gagatggatt gaagccgatc ctgcaggtgg ccagcgactg
     7021 gcaagagagc ttcacgtcgt ccggaatccc gtgcggcgcg atcaccggtc tcaccgcgca
     7081 catgcaaaac cgcttcgtgc gcaccaagta ttgcatgccg gcgattgcct ctcggatcaa
     7141 ggatatcgag tcgaagtatt acttggggtt aaggttttcg atctatcgcg acctggggac
     7201 gatcatcgca gccaatccga gcacaatcct ggcgatggcc cgtctgggtg atcggcagaa
     7261 ggaggtgcta attcgcgatc tggccgacgg caccatcgac gcccgatggg aaatcccagc
     7321 tgatatccgg cggcacttga gccgcaaaac gcgccgccgc cacaaagcgg tcgcgcgcgc
     7381 attcgagaag attgcgaatg acaccggccg actcctccct aaggattact ggcccaacct
     7441 tcagtttctg tccaactgga tgggtgggac catgcgagca tatttgcgtg gctatcctga
     7501 gtttttcggc gagacgcctg tccgggacgt cggcttgatc gcgtcggaag ggagaatgac
     7561 gatcccgatc gaggacggaa cgccggcggg catactcgat gtgcgccacc attattttga
     7621 gtttatccct gaggaagagg gagatcgcga gctacccgac accgtcgagg ccgccgatct
     7681 gatcgagggg cgaaactatt tcatcgtgct gacaaccgcc ggtgggcttt atcgatacaa
     7741 catttccgat ctggtccgtt gcgtcggctt tcatggtcag gctccgctca tcgagtttct
     7801 caacaagggt gcacactact caagcctcac cggagaaaag ctttccgaac accaggtgat
     7861 cgcggccgtc gaagctgccc agcgggccgc cggagttcac ttgcggtcat acctgctggt
     7921 tccgacctgg gatgagcccc ctttttattc gctcttggtc gaggaaagcg acctggtgtc
     7981 ttcggcaagc cacgaacgct tggccgtcgc cgtcgagcgt gagctccgat ctcagaacgt
     8041 cgagtatgtc agcaagcgtg acaccctgag gcttggccca gtccgcattc ttcggatcgc
     8101 cgacggttcc tgggcggaat tccagaaacg tcgactggtc aagagcggcg ggacggtcga
     8161 acagtataaa cagccacatc tggttcctga cctcgatctg atcaccagtt tccgccagct
     8221 tgaggtcgtg agctgaagcc agattccctt gagcaaacat cgaggatgtg gtaatccaac
     8281 ccagccaagg aagccgatcg tgcagattta tgattgcaac aatgcgtggc tatatgagtc
     8341 ggatctcggc aaatcgataa ggctcatggg ccgcgacgtc cagttagcac ggaagcaaaa
     8401 tgactggctc tgccgggtga acgatcgtgg aggggggcag agccctcggg gatgaacgag
     8461 ctccacggaa ggattgagga acgatgaaca cggcgagaga ttgcgtgcgc tcgctgtcgc
     8521 cctggatggt ggcgtgcctg ggactgggtt tgtgtctggt tgacgcggga tgctcgggga
     8581 tctcggggct gcgatccgtt ggaaccgaac ggccttcgct gctcagcttc tgggaccacc
     8641 agggtccggg ctcacccacg cctgataacg acgcctacgc gcaagcaatg cggccgagtc
     8701 gcgcgcgtgc cgaggcgatt gccaagcgga gtgattcgcc agcggttgat ggagaagagg
     8761 gctcgacgac aagagacggc atcgagctcg ccgaggcggc cgagccgata cgcagttccc
     8821 agcaccctca cgcgacaaac tcggcggatc cgagtgtgcg ggtttcgtta ggggctcccg
     8881 aaccgctacc aggcgttgct ctggccgaca gtgcggaaaa ccgaagccaa aaggttgccg
     8941 cgacgggccg agcgccctcg tggaaacgtg agaaaactga ttcatcgctc gccagcggtc
     9001 ttccgctctc gcagccggcc gatggtgacg acgttaagaa tcaagccccc tccgtgatca
     9061 ccgaacgaga gcaagccgcg ggtcccgcgg aaacct
//
LOCUS       DATAQL010000929         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_706283, whole genome shotgun sequence.
ACCESSION   DATAQL010000929 DATAQL010000000
VERSION     DATAQL010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706283"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VHS97_16185"
     CDS             complement(<1..185)
                     /locus_tag="VHS97_16185"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3449796.1"
                     /translation="MQVIACIYKQQANDCMNRPDILFKALADPSRRKLLDRLYANDGQ
                     TLSQLCEHLDMTRQSVT"
     gene            393..773
                     /locus_tag="VHS97_16190"
     CDS             393..773
                     /locus_tag="VHS97_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3449797.1"
                     /translation="MADSEIRAALDRHWAASDANDFEVEHQIYREDAVLEYPQSGERI
                     RGRRHIQASRTAQPNQKRFAVRRILGAADLWVTEFILTYDGQPSYTVSIMEFEEGKVA
                     RETQYFGDPFEPGPSRAQWVERMR"
     gene            805..1746
                     /locus_tag="VHS97_16195"
     CDS             805..1746
                     /locus_tag="VHS97_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3449798.1"
                     /translation="MAAPDTLRTAKIPLVNGSGAIPVLGFGTLIPDPVATKQATKAAL
                     EVGFRHFDCSERYRNEQAVGDAMQEMFKAGPIRRTDVFVTTKLWNNNHRAERVKPAFE
                     ASRQRLQLDYVDCYLIHTPFAFQPGDDQDPRDGHGRVLYDSGVTLAETWRALESLVDE
                     GRCRSIGLSNVNLEKLQEIVEVARIKPAVVEVESHPYLPEWDLLDYCRRQGIVLLAFA
                     ALGHGMEPRLLDDPAIVAIARRLGKTPAQVVLAWAIQRGTALLTTSVTPHRIRENFDV
                     STIPEDAMQEIRDRVATTIRFNSVVKTGVPGFIPGRR"
BASE COUNT          368 a          607 c          628 g          366 t
ORIGIN      
        1 tgggtgacgg actgccgtgt catgtcgagg tgctcgcata gctggctcag cgtttgcccg
       61 tcgttggcgt aaagccgatc cagcagcttg cggcggctgg ggtcggccaa cgccttgaac
      121 aggatatccg gtctattcat gcagtcattt gcctgctgtt tatatatgca ggcaattacc
      181 tgcatgtcaa gacgcctcgg acgattatcg gatcgccttg cattcccgat tgcgggaccg
      241 gccggttacc acgttgagcg gctatgccac agcctatcag ccgcggcttg ctgcagcgac
      301 ctgccgccgc gcggataaga gctggacgat gcttcaaatc tcttgccaga tccgcactcg
      361 ccgccacgat gacggtgcga gggaggatcg agatggcaga cagcgagatt cgagcggctt
      421 tggatcgcca ctgggctgcg tccgacgcga acgacttcga ggtggagcac cagatctacc
      481 gggaggacgc cgtgctcgag tatccgcagt cgggcgagcg catccgcggc cggcgccata
      541 tccaggcctc gcgcaccgca cagcccaacc agaagcgctt cgctgtgcgg cgcatcctgg
      601 gcgctgccga cctttgggtc accgaattca tccttaccta cgacgggcag ccgtcttaca
      661 cggtgagcat catggagttc gaagagggga aggtggcccg cgagacccag tatttcgggg
      721 atccgttcga gccgggacct tcacgcgctc agtgggtcga gcggatgcgg tgaaatgcga
      781 acggcgtgac ggaggagtac aagcatggca gcgcccgaca ctcttcgcac cgcgaagatc
      841 ccccttgtca acggatccgg cgcaattcct gtcctcgggt tcggtacgct gattccggat
      901 cccgtcgcga ccaaacaggc aaccaaggct gcgctggaag tggggtttcg tcacttcgat
      961 tgttcggaac gctaccgcaa cgaacaggcc gtcggcgatg cgatgcagga gatgttcaag
     1021 gcgggcccga tccggcggac ggacgtgttc gtcaccacga agctctggaa caacaatcat
     1081 cgtgcggagc gggtcaaacc cgctttcgag gcgagtcgcc agcgactcca gctcgactac
     1141 gtcgattgct atctgattca cacccccttt gccttccagc cgggcgacga ccaggacccg
     1201 agggacgggc acggccgggt tttatacgac tctggagtga cattagcgga gacatggcgc
     1261 gcattggaga gcctggtgga cgagggccgg tgcaggtcga tcggcctgtc gaatgtcaat
     1321 ctggagaaac tgcaggagat tgtcgaagtc gcgcgaatca agccggcagt cgtagaggtc
     1381 gaatcccatc cgtatctccc ggaatgggat ctgctcgact attgtcgacg gcaggggatc
     1441 gtactgcttg cgtttgccgc gttggggcat gggatggagc ccagattgct ggacgatccg
     1501 gcgatcgtcg ccatcgcccg gcgcctcggc aaaacccctg cccaggtcgt gctcgcctgg
     1561 gcgatacagc gcggcaccgc tctcctgacc acttcagtca cgccccaccg catccgggag
     1621 aacttcgacg tctcgaccat accggaagac gccatgcagg aaatccgcga cagggtggcg
     1681 acgacgatcc gcttcaattc ggtcgtgaag accggcgtgc ccggctttat tcccggtagg
     1741 cgctagagcc attgctggga gcgcgccact ccgtggcgct catggtcttt cggaccgcgg
     1801 gcgttcccgc ccgctcatga atgcgggcca gaggcccgcg ctccgattaa gaagcgccac
     1861 ggagtggcgc gctcccagca atgattcctc agccggtgcg ccgggcaggt gaccagccat
     1921 aggccttggc gcaggcgccg cccatcagca tggcgcgttc gctatcgct
//
LOCUS       DATAQL010000930         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3608304, whole genome shotgun sequence.
ACCESSION   DATAQL010000930 DATAQL010000000
VERSION     DATAQL010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608304"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VHS97_16200"
     CDS             <1..913
                     /locus_tag="VHS97_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX3449799.1"
                     /translation="LTLKLLSFEPTGAIVAAPTCSLPEAIGGQRNWDYRYTWIRDAAF
                     TLYGLLRIGFTDEAAQFVEWLKDRWQEADDDGAGPLQLMYGIDGRSELTEQTLDHLEG
                     YRGSSPVRIGNAAHSQLQLDIYGELMDAAYLYNKYVAPIGYDGWRRLRRLLDWLCNHW
                     QRPDDGIWEVRGGSRHFVYSKFMSWVALDRGLRLADKRSFPADRARWLKVRDEIYEEV
                     MSQGWNADRKAFVQSYGSDALDASTLLMPLVFFMAPNDPRMLSTVDAIRRSVAAGGLA
                     ADGLVYRYDPSAALDDLEGREGTFNIC"
     gene            983..1159
                     /locus_tag="VHS97_16205"
     CDS             983..1159
                     /locus_tag="VHS97_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449800.1"
                     /translation="MFEQVLGYANHLGLYGEQTGASGEALGNFPQAFTHLGLISAAFN
                     LDRALDQRHSGRRV"
     gene            complement(1383..3551)
                     /locus_tag="VHS97_16210"
     CDS             complement(1383..3551)
                     /locus_tag="VHS97_16210"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3449801.1"
                     /translation="MNDASTMEIDFEPERYELREEPAYQFELKRRDFLKTLGGGILVF
                     YMLDAAEAQPPGGGRRRGGGGGQAAVPQDIGAWLHIGENGRIAVYTGKAEVGQNIRTS
                     LTQAVADELHAAPGSIDLVMADTQLTPFDAGTFGSRTTPDMSRRLRRAAAAAREALID
                     LAAEHWKSERSLLTAADGAIVNTKTQESLEFGKLTHGHKLTKTVADNFPVSTPEQWTI
                     AGHSAAKVDGGAFVTGKHQYPSDIRVPGMWYGKVLRPPTIGGTLVSIDTSLAKAMPDV
                     AVVHDGDFVGVASFTEAQAERALAAIKTEWKPGPRISSKQLFDDLKQEGRNARQGRDA
                     SGQRAGSVKEGLDAADVRLEQTYTIAYIAHAPLEPRAAVARWDNGKLTVWTGTQRPFG
                     VRGELTAAFRVADDAVRVIVPDTGSGYGGKHTGDAAVEAARLARAAGKPVRLVWTREE
                     EFTRAYFRPAGVIEVTSGVKRDGTLTAWEYHNYNSGASGIRTFYEVPNQLVAFQSVPY
                     PLRQGSYRALASTANHFARESHMDELAHLVKIDPLEFRLKNLKDARLRAVLEAAAASF
                     GWGKKSEADHGIGIAAGSEKGSYVATCAEVAVDRSSGRVQVVRATTAFECGAVVNPDH
                     LKNQVEGAMVMGLGGALFEAIQYEDGRITNGRFSRYRVPRFRDTPLIEVVLLDRKDLA
                     PAGAGETPIVAIAPAIGNAIFDAAGVRLRSLPMVPQGLRV"
     gene            complement(3548..4027)
                     /locus_tag="VHS97_16215"
     CDS             complement(3548..4027)
                     /locus_tag="VHS97_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3449802.1"
                     /translation="MPDVKQLLINGAMVQVSAEGDRSLLGVLRDELCLTGAKYGCGEG
                     RCDACTVLVDGEPVRSCVTKLSAVATHEIMTIEGLEKDGKLHPLQQAFLEVGAMQCGY
                     CTCGMIMSGVGLLRKNPHPTREEIVEHMNGNICRCGTYPRIIAALATAAKSIKEARR"
BASE COUNT          753 a         1301 c         1292 g          877 t
ORIGIN      
        1 attaacgctc aagctcttgt ctttcgagcc aaccggcgct atcgtggcgg ctcccacttg
       61 cagcttgccg gaggcgattg gcggacaacg caactgggac tatcggtata cctggattcg
      121 tgacgcggcc ttcactctct acggcctgtt gcgcatcggc tttaccgacg aggccgcgca
      181 gttcgtggag tggctcaagg accgctggca agaagccgac gatgacggcg ccggtccgtt
      241 gcaactgatg tacggcattg atggacgatc cgagctcacc gaacaaacgc tcgaccatct
      301 ggagggatat cgtggctcgt cacccgtgcg gatcggcaac gcagcccata gtcagttgca
      361 gctagacatc tatggtgagc tgatggacgc cgcctatctg tacaataagt acgtagcacc
      421 gatcggatac gacggctggc gccgactgcg acgactgctg gactggctat gtaatcactg
      481 gcagcgccca gacgacggaa tctgggaagt gcgcggcggc tcgcgtcact tcgtttattc
      541 gaagttcatg tcgtgggtcg cgcttgaccg tggtctgcgg ctggccgaca aacgctcttt
      601 cccggccgac cgggcacgat ggctgaaggt gcgagacgag atttacgaag aggtcatgtc
      661 tcagggatgg aacgcggacc gcaaggcttt tgttcagtcc tacgggtccg atgccctgga
      721 cgcttcgacg ttgctcatgc cgctggtgtt tttcatggcc cccaacgatc cgcggatgtt
      781 gagcaccgtt gacgcgattc gccggtcagt ggcggcgggt ggcctggctg ccgatggttt
      841 ggtttaccgc tacgatccca gcgccgccct cgacgatctg gagggccgcg aaggaacctt
      901 caacatatgc tagttttggc tgatcgaagc actaactcgc gcgggccgca ctgatttaca
      961 gagactggaa gatgcgcgac tcatgttcga gcaagtgctc gggtacgcca atcaccttgg
     1021 cctttacggc gagcaaacgg gcgcaagtgg tgaagcgctg ggaaacttcc cacaagcctt
     1081 tacacacctg ggcttgatca gcgctgcgtt caatctcgat cgtgcgctcg accaacgtca
     1141 ttcaggccgt cgcgtttgaa tgagtgaacg acgatggttt gttcaccaga acatcgggag
     1201 aaatgtgcct gattgctcac tgaccacccc ccttcacaag ggggggaagt ccacgcggcg
     1261 ctcgacggag tggcgcaacg aaaatgggag gaatgcagcg gtgtcgtggg aggaatgcag
     1321 cggtgttgcg tcaccaaacc tccatcaacc cagccacacc ccctggactc atgcacatct
     1381 cttcagactc ttaatccctg cggcaccatc ggcagtgatc gcagtcgcac gccggcggcg
     1441 tcgaaaatgg cgtttccgat cgccggtgcg atggcgacga tgggtgtttc gcccgctcca
     1501 gctggcgcta ggtccttgcg gtcgagcaag accacctcga ttaacggcgt gtcgcgaaac
     1561 ctgggtacgc gataccgtga aaagcggccg tttgtgattc ggccgtcctc atattgaata
     1621 gcctcgaaaa gggcgcctcc caggcccatg accatggccc cttccacctg gtttttgaga
     1681 tggtcggggt tgacgacggc cccgcattcg aaggcggtcg tcgcgcggac gacctgaacg
     1741 cggccgctgg accggtcgac agcaacctcg gcgcaggtgg ccacgtagct tcctttttcg
     1801 gatccggccg cgatgccgat cccgtgatcc gcctcgctct tctttcccca gccgaacgac
     1861 gcggcggcgg cctcgaggac ggcgcgtagc cgtgcgtctt tgaggttttt cagccggaat
     1921 tccagcgggt cgatttttac caagtgcgcc agttcgtcca tgtgcgactc gcgggcgaaa
     1981 tgattggccg tggaggccag cgcccggtaa gagccttgtc gcagaggata aggcacgctc
     2041 tgaaaggcga cgagttgatt gggcacctca tagaaggtgc ggattccgga cgctccggag
     2101 ttgtaattgt gatattccca tgccgtaagc gttccgtcgc gcttcacgcc gctggtaacc
     2161 tcgatcacgc cggctggccg gaagtaagct cgggtgaatt cttcctcgcg ggtccagact
     2221 agcctgaccg gcttgccggc cgcgcgtgcg aggcgtgcgg cttcgacggc cgcgtcgcct
     2281 gtgtgcttgc caccgtagcc cgaccctgtg tccgggacga tcacccgcac ggcgtcgtcg
     2341 gccactcgaa atgccgcggt cagctcaccg cgcacgccaa aaggccgctg cgtgccggtc
     2401 cagactgtga gcttgccgtt gtcccaccgc gcgacagccg cgcgcggctc aagcggcgcg
     2461 tgggcgatgt aagcgatcgt gtaggtttgt tcgagacgca cgtccgcggc gtcgagacct
     2521 tcctttaccg agccagcacg ctgtcccgag gcgtcacgcc cttgacgggc gttccgccct
     2581 tcctgtttca ggtcgtcgaa tagctgcttg ctcgaaatcc gggggcccgg cttccactcg
     2641 gtcttgatcg ctgccagtgc acgttcggcc tgggcttcgg tgaacgaagc gacgccgacg
     2701 aaatcaccgt cgtgcaccac cgccacgtcc ggcatcgcct tggccagact cgtgtcaatc
     2761 gataccagcg tgccgccgat cgtcggtgga cgtagaacct tgccgtacca catccccgga
     2821 acccgaatgt cggatggata ttgatgtttc cccgtgacga aggcaccacc atcgaccttg
     2881 gccgccgaat ggccggcgat cgtccattgc tcgggcgtac tgaccgggaa attgtcggcg
     2941 acggtcttcg ttaacttgtg cccgtgcgtc agtttgccga attcgagcga ttcctgagtc
     3001 ttggtgttga cgatggcgcc gtcggcggcc gtaagcagcg aacgctcgga cttccagtgc
     3061 tccgccgcca agtcgatcag ggcttcgcgc gcggcggcgg ctgcgcgccg cagtcgccgc
     3121 gacatgtccg gcgtggttcg gctaccgaag gtccccgcgt caaatggggt cagttgcgta
     3181 tcggccatga ctagatcaat cgagccaggc gcggcgtgca attcgtcggc cacggcctgc
     3241 gtcaatgacg tgcggatgtt ctggccgact tccgccttgc ccgtatagac ggcgatccgt
     3301 ccgttctcgc caatgtgtag ccacgcgccg atatcctgcg gcacggccgc ctggccgcct
     3361 ccgccgcctc gccgccggcc gccaccgggc ggttgggctt ccgcggcgtc gagcatgtag
     3421 aacaccagga tcccgccacc gagcgtcttc aaaaagtccc gccgcttgag ctcgaactga
     3481 tacgcaggtt cctctcgcag ttcatagcgc tcgggttcaa agtcgatttc cattgtggat
     3541 gcgtcgttca tcgtctggcc tccttgatgg acttggcggc cgttgcgagt gcggcgatga
     3601 tccgcggata ggtgccacag cggcagatat tgccgttcat gtgctcgacg atttcttcac
     3661 gcgttggatg ggggttttta cgcaagaggc cgacgcccga catgatcatg ccgcatgtgc
     3721 agtagccgca ttgcatggcg ccaacctcca ggaaggcctg ttgcaatggg tgcagcttgc
     3781 cgtctttctc caggccctcg atggtcatga tctcatgcgt ggcgacggca gagagttttg
     3841 taacgcagga acgcactggc tcgccgtcga ccagcacggt gcaggcatcg cagcggccct
     3901 cgccgcagcc atacttggca cccgtgaggc aaagttcgtc acgcagcacc cccagtaacg
     3961 accggtcccc ctcggcactg acctggacca tggctccgtt aattaggagc tgtttgacgt
     4021 cgggcatcag tgtggtcctc ccgatcaagg aattgcgatc ttccggaaat ttctttgttg
     4081 agcgtcacga cgccatactg ttacggcttt acagccataa ccgcatcgag aggcgaaggt
     4141 gtcgtcacgc ctttgacgtc gacgaagcct acctgattaa gaagcgattt atactcgctc
     4201 aaggtccgtt ctttcccctc ggt
//
LOCUS       DATAQL010000931         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2536843, whole genome shotgun sequence.
ACCESSION   DATAQL010000931 DATAQL010000000
VERSION     DATAQL010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536843"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VHS97_16220"
     CDS             complement(<1..1404)
                     /locus_tag="VHS97_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bZIP transcription factor"
                     /protein_id="HEX3449803.1"
                     /translation="MGDERDPGMETSRPPRHRQGAFWTERSLLALIIASLAVTFNLLL
                     AVHRQAIVPIGAPDSETKTPQITPPTSSPKPVARSQVTAGDSGGIKPAIAQSRPERPK
                     NQPAPEDPTKMAVAGLTNATGQEIAEAERSDGRAMALETARRASVAESRRWKRRELLV
                     RQQIASLTDRANQLESETESLDAERDVLAHERDALKAALTKAGRRSGFAVLPYKGPNG
                     TWRRPIVLECTAGSVKLQPRGLTFSSMELSPLINPRSSPLVRAVAGELLHVQASDTPD
                     GAPAVPYLVFLVRPSGIRQYYEARTCLEPLGIAFGYELIEQDLVVDIPNFDNLATWDG
                     SVPLDMPLETAPGRRETVAEKSRSVGGDGTSAGSLSGSQANRPSFGAWPERSDGGGHA
                     SSGDQGSRDAASPEDFVWPTRARQNAASSRPSSRSDAADSNEGPINREDVGRLAPGDV
                     GSPSGCPPSGRGTGPFGT"
BASE COUNT          316 a          573 c          552 g          362 t
ORIGIN      
        1 agtgccgaat ggtccggtcc cacgaccgct gggtggacat cccgatggcg atccgacgtc
       61 gcctggagcc aagcgtccca cgtcttcccg attgataggg ccttcattcg agtcagcggc
      121 gtctgacctc gagctcgggc gagaactggc ggcgttttgc cgcgctctgg tcggccagac
      181 gaagtcttcc ggacttgccg cgtctcgact gccttggtcg ccgctagaag catggccacc
      241 gccgtccgat cgttccggcc aggcaccaaa tgatgggcga ttagcctgcg agccagatag
      301 cgaacccgcc gacgttccgt cgccgccgac agatcgtgac ttttccgcaa cagtctccct
      361 acgccctggc gcggtctcca ggggcatatc cagtggcacc gatccgtccc aggtcgcaag
      421 gttgtcgaag ttggggatat cgaccaccag gtcctgctcg atcagctcgt aaccaaacgc
      481 gatgcccagc ggctcgaggc aagtcctggc ctcgtaatac tgacggattc cgctgggacg
      541 aaccaagaaa accaggtacg gcacggccgg ggctccgtcc ggcgtgtcag atgcctggac
      601 gtggagcaac tcgccagcga cagcgcgaac aagagggctc gagcgaggat tgatcagcgg
      661 tgaaagttcc atcgatgaga aggtgagacc gcgcggctgc aacttgacgc tgccggccgt
      721 gcattccaag acgatggggc gccgccaggt cccgtttggt cccttgtagg gcaagaccgc
      781 gaatccggac cgacggcctg ccttggtgag cgctgccttg agggcgtcac gttcgtgggc
      841 cagcacgtcg cgctcggcgt cgagcgattc ggtctcgctt tccaattgat tggcgcggtc
      901 ggtaaggctg gcgatctgct gccgaaccaa gagctctcgc cgcttccacc gtcgcgactc
      961 tgccaccgaa gcccgccgcg ccgtttccaa cgccatggcg cgaccatctg accgctcagc
     1021 ttccgcgatc tcttgacccg tggcattcgt tagtccagcc acggccattt ttgtgggatc
     1081 ctctggggcc ggttggtttt tgggacgttc cggccgactt tgggcaattg cgggctttat
     1141 tcccccggaa tctcctgcgg taacctggct gcgcgcgact ggctttggcg agcttgttgg
     1201 gggtgtgatc tggggcgtct tagtctcaga atcgggcgcc ccgatcggga cgattgcttg
     1261 gcgatgtacg gccagcagca ggttgaatgt gacggccagg ctggctatga tcagggcgag
     1321 gagcgaccgc tcggtccaga acgcaccttg acggtgcctc ggcggccgcg atgtctccat
     1381 ccctgggtcg cgctcgtcgc ccattcgagc cggcatcctt gtccggacca gtgatcccgc
     1441 ggttctcgaa cgtttcgcgt atgtcttatc ttatgaaatc ttgccaatct tcgcgatgcg
     1501 gcttcgcgaa ccagcacttg tatcagatcg gagttttgtc caacccggct tgagttcctg
     1561 gtcgacgaga aaacgcgcac tcgacccgtc catagcacgt tttgcgaagc atccccagtt
     1621 tgaccagctc ccccaaaaca acgcatctac gtacgaatcc tgagcctcca cgggctcgcg
     1681 gcaggtcatg cgtgctcgga cgggaaaagc gtattcgcga acgcacagct cagaccgaca
     1741 gcattaatga ttcattcccc gcgcaagtca acggccatcg cggcgggcag cgccggagcc
     1801 gtc
//
LOCUS       DATAQL010000932         4211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2843143, whole genome shotgun sequence.
ACCESSION   DATAQL010000932 DATAQL010000000
VERSION     DATAQL010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843143"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1204
                     /locus_tag="VHS97_16225"
     CDS             407..1204
                     /locus_tag="VHS97_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449804.1"
                     /translation="MIYKRTLIPAAFVSIGFVGLAISLCSGADEPPPRPRPGLTAKQT
                     KEAVKIAQGAMVELRKKTEGASAPGADLREYVVGVELLATSVASSPSATKPTTPAETE
                     PAETKAPAKEKENGKESETEKENRPIPGPRAIVTSYRYFDDITVFSTIDLATGQVVKV
                     EAAQHLRTPLSQVEFDEAQEMARDKVDQVKQLYERFGDQISVYPQFSQFTIKDDPRIH
                     RVVHLNYRVGKRDLSYPRPRVDLTTRQVELPDPEPAEVAPRVRRRSP"
     gene            1268..2593
                     /locus_tag="VHS97_16230"
     CDS             1268..2593
                     /locus_tag="VHS97_16230"
                     /inference="COORDINATES: protein motif:HMM:NF013354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449805.1"
                     /translation="MLLRSHRTGAFFAFCLLAAMPSAASAQPFFFRQGLVDPPAQEVT
                     AEFLFGESGGIEPRKGTSWKVQFARGLHKGLYITGAWFKRDLGEEWIKILNDARVAEL
                     FVPYHQSSFIRFFDLTSFSFPLAEVKSEDTGAFGTLMPAFQGDRYPTVVKEVRDRGLA
                     WKDGVHGVRRGRELVLWAGLEAGNYMYIMSYAFQDDGTIAFRVGATGQNLPGARYQAH
                     VHSAHWRIDIDLFDGKKNSAMLMRHIEDPASYAAEDVKDPFNGGIEGGVDWDPKEFSM
                     IRVQCEKQNARGETIGYDLMPLRYGTPRHNEEFTHHDLWVSQSHPERPMEFIFSNLPN
                     IVKDEEPVEQTDIVLWTNSAVHHEPRHEDGKPNSAPRVWPGDDAWEGSALLMWSGFDL
                     RPRNLFDRTPFYPYPPPTPARPGENARPNAAPGDGNGEPGAPAGAVSRR"
     gene            complement(2625..3434)
                     /locus_tag="VHS97_16235"
                     /pseudo
     CDS             complement(2625..3434)
                     /locus_tag="VHS97_16235"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
     gene            3631..4104
                     /locus_tag="VHS97_16240"
     CDS             3631..4104
                     /locus_tag="VHS97_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX3449806.1"
                     /translation="MSLVVRSGAFGSGKPILRRHTGDGEDLSPPLSWSSLPATARELA
                     LIVDDPDAPTPEPWVHWVIYSMAATTEGIAEGVPPVARPSFPAMFTQGKNSWSTIGYR
                     GPAPPKGHGVHRYFFKLYALDKALDLPAGLDKPSLQNAMKGHIVAEGELMGTYQR"
BASE COUNT          917 a         1302 c         1279 g          713 t
ORIGIN      
        1 caatcgacga cacctgcaaa gctcacctcg aggaatgccg gcaccggatc agcaaagtcc
       61 tcgaggccaa cctcgacgtc cgcgagccgt gagcgcgagg ggcatcacga cggtggcccc
      121 ctcgccgata tctggagcag atgatcgaat tcggtttggg ttgcgctaaa caggcaagag
      181 tgggtggctg gggtcgtcgt cagaaagggc gacgccccca gtgacgacca ctgtgggcta
      241 gccggattga agccggcttc gaccacagcc acccaccgct caaggggcgc caacaggcgc
      301 aacaaaaaag gttgaacgtc cgggcgggga agacctacag ttcatcagtc ccacccgcag
      361 ggccggcgag cgggcctcaa ggaccgtgag tcgcgaggga accgagatga tttacaaacg
      421 aacattgatc cccgctgcct ttgtgagcat tggctttgtt ggcctggcga tttctctgtg
      481 ctctggtgcc gacgagccac caccgcggcc gcggcccggg ttgaccgcca agcagaccaa
      541 agaggcggtc aagatcgccc agggcgccat ggtcgagctg cgcaagaaga cggaaggagc
      601 gtccgccccc ggcgccgact tgcgtgagta tgtggttggt gtcgagctgc tggccaccag
      661 cgtagcgagt tcgccgtccg caaccaagcc aacgactcct gccgagaccg aaccggccga
      721 gaccaaagcg cccgcaaagg aaaaggaaaa cgggaaagag agcgagacag aaaaagagaa
      781 ccggccgatt cctggcccgc gcgcaatcgt gacgtcgtat cgctatttcg acgacatcac
      841 ggtcttttcc acaatcgacc tggcaaccgg ccaggtcgtc aaggtcgagg ccgcccagca
      901 tcttcgcacg ccgctttcgc aggtcgaatt cgacgaagct caagagatgg cgcgtgacaa
      961 ggttgaccaa gtgaaacagc tctacgaacg ttttggcgac cagatcagtg tctatcctca
     1021 attcagccag ttcacgatca aggacgaccc gcgaatccat cgggtcgtgc acttgaatta
     1081 tcgagtgggc aagcgagacc tgagctatcc gcgcccgcgg gtcgacctga cgacgcggca
     1141 ggtcgaactt cctgaccccg agccggccga ggtcgcgccc cgggtgcgcc ggcggtcgcc
     1201 atgatcgaga gctcgacgcg agacgactga gtcgacacca ggcttttcgt caatgaaggg
     1261 atcccgcatg ctgcttcgtt ctcatcgcac cggtgcgttc tttgcgttct gcttgctcgc
     1321 ggccatgccg tcggccgcga gcgcgcagcc gtttttcttc cgccagggct tggtcgatcc
     1381 cccggcgcag gaagtgaccg cagaatttct cttcggcgag tcgggcggga tcgagccgcg
     1441 caaagggacg tcttggaagg tccagtttgc ccgcggtctg cacaaggggc tgtacatcac
     1501 cggcgcgtgg ttcaagcggg atctgggcga agagtggatc aagatcttga acgacgcgcg
     1561 cgttgccgag ctcttcgtcc cctaccatca gtcgagcttc atccgtttct tcgacctgac
     1621 gagcttttca tttcccctgg ccgaggtcaa gtcggaagac accggcgcgt tcggcacgct
     1681 gatgccggcc tttcaagggg accggtaccc cacggtcgtc aaagaggtac gcgaccgcgg
     1741 gcttgcctgg aaagatggcg tgcacggtgt ccgacgcggc cgcgagctgg tactctgggc
     1801 cgggctcgaa gctgggaatt acatgtatat catgtcctat gcatttcaag acgacggcac
     1861 gatcgccttt cgcgtgggcg cgacagggca aaacctcccg ggcgcccgct atcaagccca
     1921 cgtgcatagc gcccactggc ggatcgacat cgacctattt gacggcaaga aaaactcggc
     1981 catgctgatg cggcacatcg aagatcccgc aagctatgcc gctgaggacg tcaaggaccc
     2041 gttcaatggc gggatcgagg ggggagtcga ctgggatccc aaggaattct cgatgattcg
     2101 cgtgcaatgc gaaaagcaaa acgcgcgggg cgagacgatc ggttacgacc tgatgccttt
     2161 acgctatggc acgcctcggc ataacgaaga gttcacccat catgacctct gggtcagtca
     2221 gtcgcatccc gagcggccga tggagtttat cttctcgaat ctgccgaaca tcgtgaagga
     2281 cgaggaaccc gtggagcaga ccgacatcgt gctctggacc aactcggccg tccaccacga
     2341 gccgcggcac gaagacggca agccgaactc agcaccacgc gtctggccgg gcgacgacgc
     2401 ctgggaaggc tcggcgctcc tcatgtggag cggctttgac ttgcgccctc gcaatctctt
     2461 cgatcggacc cctttttacc cttatccacc gccgactccg gctcgtccgg gtgagaatgc
     2521 aagacccaac gccgccccag gcgacggaaa tggggagccg ggagcgcctg ccggtgctgt
     2581 gtcacggcga tgaaattcgc gctctttctg agccagaacc aaacggctag cccgcatgca
     2641 aacagagcca cgctgatatt ctgagacaag gtcatgccgg cgaaaatagc cggctcgtcc
     2701 ccacgcaaca tttccagcgg ccagcgcgtg atcggatagg cgatcatgag tacggccatc
     2761 gtttcgccag cggcacggcg acggcgggaa tacaccagca ataagccgag cagcgcgaac
     2821 ccagcaatcg acgcgtagag ctgggtcggg tgcaccggca gtgaaaacgc cgcagtcggc
     2881 gaaatcaagc ccgcgtccac ctggcgaacc caggcatgcg aaccggctcg gaaacggatt
     2941 gcccatggca aatcggacac cgccccgtaa cagcagccgt tcaagaagca gccaatacgg
     3001 ccgatggcga ttcccatcgc gaccgccaaa gaggccgcgt cggccatccg ccgaaacgga
     3061 aacggccgcc ggagccagta aaggatcgat ccggtgaggc cgcccaaaat gcaaccgtaa
     3121 aagttgttgc cgccctgcca gctccggaag atgtcgccca gactgtggat cgcttccgga
     3181 tgggaaatca cgaagacgcc gcgcgcgcca atggccccac ccaagaaaag ccacgccgcc
     3241 agttcgtaga cgctgttggg atcgatttga tctcgtcgag cccgccagac acagattccc
     3301 aacgcggcga agcaagcaat caagatcatc acgccgtagc tatggatctt gagttcccag
     3361 cccggaatct caaataagat cgaccgcatc cttgcgtctc ctcgcgccca aagtggccgc
     3421 gcccacggtc ccatggcggg caggatgaca gggaagggga aatcgttcaa tatcagaatc
     3481 gctagcactc atgcatgcga gagcgaggtc ggcgggtact ttcgagcgct cgtcacgact
     3541 cgctgatcgc ggaaatcgcg ttagaatggc gctggccagc aagcgcgagt cgcgcgagca
     3601 gttgatgctt ctttctatgg gagcaaagtc atgagtctcg tcgtccggag cggggccttt
     3661 ggcagcggaa aaccgattct tcgcaggcac accggcgatg gcgaggatct ctcgccgcca
     3721 ctatcatggt caagcctacc tgcgacggct cgggaacttg cgctgattgt tgacgacccc
     3781 gacgcaccga cacctgagcc atgggttcac tgggtcatct acagcatggc ggcgacgacc
     3841 gaagggatag ccgaaggagt tccacccgtg gcacggccga gtttccctgc catgttcact
     3901 caaggtaaga actcatggag cacaatcgga tatcgcggcc ccgcgccgcc gaaaggtcat
     3961 ggtgtccatc gctatttttt caaactttac gccctggaca aggcgctgga tctgccggct
     4021 ggactggaca agcctagcct gcagaacgcg atgaaaggac acatcgtcgc cgagggtgaa
     4081 ttaatgggaa cttaccagcg atagccacgc aaaagaggcc agccctacgt gccggacaag
     4141 cgtgctcatc gcggtcccga tccgcgcgat gccgaggcct ttggtccgcc caatcggtcg
     4201 gccctgcgcg g
//
LOCUS       DATAQL010000933         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2537177, whole genome shotgun sequence.
ACCESSION   DATAQL010000933 DATAQL010000000
VERSION     DATAQL010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537177"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            635..1819
                     /locus_tag="VHS97_16245"
     CDS             635..1819
                     /locus_tag="VHS97_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX3449807.1"
                     /translation="MFDLRAVQRSLGEFGVDGWLFYDFRGGNVLARRVLGLDQKPPGS
                     RRFFYYVPASGEPRKLVHRIETAALDDLPGDKRVYLKWQDLESGVAQLLGGSRRVAME
                     YAPRVSNPYISRVDAGTIELVKGCGVEVISSGDLIQQFEATWDDSQWQMHLDAEKVTT
                     SAFDLAWGLIGERVRAGGTIRETEVQAAIMDHFHRHGLTTYSPPIVGVGPHSGDPHYE
                     PIAGKDAAIGPGDLVLIDLWAKLDRPRSVYSDLTRMGFVGNVVPARYEAIFAIVAQAR
                     DAAISCVREAYAAGRPLHGWEVDAAGRRVIDAAGYGPHFIHRIGHNIGQEVHGNGANM
                     DDLETHDERLVLRRTCFSIEPGIYFDEFGVRSEVNVFVDGNGGVHVTGGLQTKILLIL
                     QE"
     gene            1824..2459
                     /locus_tag="VHS97_16250"
     CDS             1824..2459
                     /locus_tag="VHS97_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HEX3449808.1"
                     /translation="MPCTEFVTAGRRPWVLMAAIAGFAAGSVVQSVPFMALAAAGADG
                     HGAKADPAAKAPVAEVMHCPLAFEGIHLLKEMPEVSKVAYHYCKPVGDDLAQCLLYDG
                     TGPDARLIGVEYLVPDTVFRSMPPEEQAYWHDHKYEVDAGLLRSLTQTGEEEKATLAK
                     VRPMYGKIFHTWSTGKTYPRGPAKLFWTVTGQEPFVLPSQAQPALSNSANR"
BASE COUNT          474 a          766 c          806 g          512 t
ORIGIN      
        1 accgcgccgg cgattctcag cgctatcaac acttttttga ccggcatttc gacgacaaca
       61 agttcgagcg cctcggccag agcccaggcg gcttctccgg tcatcagcgt gaggaacgcc
      121 ggcgccatcg gcagacctcg gcgtcgccag gcgaagacag ccagtgttac tgatacagcc
      181 gcggcgatag caaaaggaat cgcctcgaaa ttgccttggg gcgtcatgat cctgcttcca
      241 gccaggctcg ggcagtggcg agcgttggct cgctgcgtta gagaggcgac taattcagtc
      301 tgggcggatt aatcggccaa ggtcgggcgt cttcgcgcgt ggcaccgaac cagtaaggcg
      361 ctcgtcaccg tgccacttga aacagagagt gaaacatcgt gtccccggtg ctgctgatca
      421 ccgtcgacca cctgcttcat gaggtcctca cttgagggtt gcattatcat acacaattcg
      481 tcccgcaacg gcgaggggta tctccgattg tacacttgat tgtcacgtcg gcgaactgcg
      541 ttctggcccg cggcgattga cgcccatata ctgttggagc attgctagtg taccgatcac
      601 ttttctccct gagatgctgg ctggtggtgg attcatgttc gatttaagag ccgttcagcg
      661 cagcctgggt gagtttggcg tcgatggctg gctcttctac gatttccggg gcggcaacgt
      721 gctggcgcgg cgagtgctgg gcctggatca aaaaccgccg ggcagccgcc ggttttttta
      781 ctacgttccg gcttcgggag agccgcgcaa gctggtccac cgcatcgaga ccgctgccct
      841 cgacgatctc cccggcgaca agagggtgta tctcaagtgg caggacctgg agtcgggagt
      901 tgcccagcta ttaggcggca gtcgccgcgt cgcgatggag tacgccccgc gggtgagtaa
      961 cccctatata tcgcgggtcg atgccggcac gatcgagctg gtgaagggct gtggtgtcga
     1021 agtgatctcg tcgggcgacc tgatccagca attcgaggcg acctgggacg attcgcaatg
     1081 gcagatgcac ctcgatgccg aaaaggtcac aacgtccgcc ttcgatttgg cctggggcct
     1141 gattggagag cgagtccggg ctggcgggac gatccgcgaa acagaggtgc aagcggcgat
     1201 catggatcat ttccatcgcc atggacttac gacgtacagc ccgccgatcg tcggggtcgg
     1261 cccccacagc ggagacccgc actacgaacc gatcgccggc aaagacgccg cgatcggtcc
     1321 cggagatctc gtcttgatcg atctctgggc gaaactcgac cgtccgcgat cggtctacag
     1381 cgacctgacc cgcatgggat tcgtgggtaa tgtcgtcccg gccaggtacg aggctatttt
     1441 cgcgatcgtg gcccaggccc gtgacgcggc gatctcctgc gtacgcgaag cgtatgcggc
     1501 gggccggccg ctccacggct gggaggtcga tgccgccggt cggcgcgtga tcgacgccgc
     1561 tggctatggc ccccacttca tccaccggat cggtcacaac atcggccagg aagtgcacgg
     1621 caatggcgcc aacatggacg atctcgagac ccacgacgag cgcctggtcc tgcggcggac
     1681 ctgcttctct atcgagcctg ggatctattt cgacgagttt ggtgtccgca gcgaggtcaa
     1741 tgtattcgtc gacggcaacg gcggtgttca cgtgaccggc gggctccaga ccaagatact
     1801 gctaattctc caggagtgaa tcgatgcctt gtacggaatt cgtgaccgcg ggccgcaggc
     1861 cctgggtcct gatggctgcc attgcgggtt tcgcggcagg gtcggttgtg caaagcgttc
     1921 cattcatggc gctcgccgcc gccggtgccg acggtcacgg cgccaaggcc gacccggctg
     1981 ccaaggcgcc tgtggccgag gtcatgcatt gcccgctggc attcgaggga attcacttac
     2041 tcaaagaaat gcctgaagtc tccaaggttg cctaccacta ttgcaagccg gtgggcgacg
     2101 acctcgccca atgcttgctc tacgacggca cgggccccga cgcccgcttg atcggggtcg
     2161 aatatttggt tcctgacacc gttttccgca gtatgccgcc ggaagagcaa gcctactggc
     2221 acgaccacaa atacgaggtc gacgccggct tgctcaggag tctcacgcag accggcgagg
     2281 aggaaaaggc gacgttagcc aaggtgcggc ctatgtacgg caagatcttt cacacctggt
     2341 cgacgggcaa gacgtacccc cgtggccctg caaagttgtt ctggacggtt acgggtcagg
     2401 agcccttcgt gctccccagc caagcccagc cggcactgtc gaattcggcc aatcggtaat
     2461 ttctagatgt ccgcctcgta acggcttggc atcggttcga gtgaatcgcg acgccttgta
     2521 ggcggtctgt gcttcatttg cgggggtgac acggccgt
//
LOCUS       DATAQL010000934         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1470219, whole genome shotgun sequence.
ACCESSION   DATAQL010000934 DATAQL010000000
VERSION     DATAQL010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470219"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1182
                     /locus_tag="VHS97_16255"
                     /pseudo
     CDS             1..1182
                     /locus_tag="VHS97_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
     gene            1249..3684
                     /locus_tag="VHS97_16260"
     CDS             1249..3684
                     /locus_tag="VHS97_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX3449809.1"
                     /translation="MESSLKTQVPRQKGTPIADAAVEAPLDPDVLRNMDAYWRAANYL
                     SVGQIYLFDNPLLREPLKLEHVKPRLLGHWGTTPGQNFIYVHLNRVIKKYDLNVIYVS
                     GPGHGGPAVVGNVYLEGTYSEIYPNISEDLSGMQKLFKQFSFPGGIPSHVAPETPGSI
                     HEGGELGYSLSHSFGAAFDNPDLIVACVVGDGEAETGPLATGWHSNKFLNPAHDGAVL
                     PILHLNGYKIDNPTILARIGREELEHLLLGYGYTPYFVEGHEPEAMHRLMATTLDKVV
                     AEIKRIQSDARERGVTERPRWPMIVLKSPKGWTGPKEVDGRPVEGTYRAHQVPLSEPT
                     EHPEHLKILESWMRSYRPEELFDETGRLIPELRELAPKGERRMGANPRANGGTLLRDL
                     RLPDFCDYAIEVPRPGNVMNEDARTLGKFLRDVIKLNAEQRNFRIFGPDETASNRLTA
                     VFEVTNKQWLGTVVPTDDRLSPDGRVIEMLSEHQCEGWLEGYLLTGRHGIFNTYEAFV
                     HVIDSMFNQHAKWLKVTRRLPWRRPVASLNILLASHVWRQDHNGFTHQYPGFIDHVVN
                     KKAEVIRVYLPPDTNCLLSVMDHCLRSRHYVNVVVASKHPSPQWLTMDQAVKHCTAGI
                     GIWEWASNDRGAEPDVVMACAGDVPTLETLAAVQLLREHIPELKIRVVNVVDLMRLEP
                     ATEHPHGLDDHDFDTLFTKDKPIVFAYHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTI
                     STAFDMTVMNDLDRFHLVADVVDRLPQLGSKAAYAKQAVRDKLIEHRQYITKYGEDMP
                     GIRDWKWTVATRT"
BASE COUNT          734 a         1208 c         1194 g          687 t
ORIGIN      
        1 gtcggctcat cgagtctcaa gtttgcgctc tacgagccgg ccgaacggcc cacacgcctc
       61 ctgtccggtc gggtcgagcg aattgggatg ccgggttcgc ggctggtcat ggttagcacc
      121 gaatgcggtc aggaggagga caacgccgtc gaggctccgg accagccagc ggcggtgggc
      181 gtgatgatcg agctactggg atacgtagtc ggtctgaaga gcctcgccgt cgtcggtcat
      241 cgcgttgtcc acggtggcaa ccgctttttc cggccggagc ttatcacgcc cggggtgctc
      301 gaggaattgc gcaggatcat cccctacgac cccgatcacc ttcccggcga gatcggagcg
      361 atcgaagcat tgcttcgcct cgaacccgac ctgcctcaag tggcttgctt tgacacggcc
      421 ttccatcacg acctcccgcg cgtagctcag atgatcgcga tcccgcggcg gtacgaggcg
      481 gccgggatcc gtcgttatgg cttccatggt ctctcgtacg cctatctgat ggaggagctc
      541 gcccggatcg ccggctctgg ggcagcccga ggccgcgtta ttctagccca cctcggttcc
      601 ggagcgagcc tggcggcggt gcgcgatggc cgttgcgtcg aaaccacgat gggcctgact
      661 gcaacgtcgg ggttggtcat gggcacgcgc tgcggtgata tcgaccccgg cctggtccgg
      721 ttcctgatgc gggctaaggg gttgacgatc gatcagttcc atgacctcgt caatcacgaa
      781 tccggattgc tcggtgtctc ggagtcgagc tctgaccttc gcgacctcct gtcgaggcag
      841 ggtcaggacg tccgcgccgc ttaggcggtc gagctgttct gctaccgagc caggactggc
      901 ctaggcgcgc tggcggcggc gcttggggga gtggatacct tggtgttcgc aggtgggatc
      961 ggcgagaact cgcccgaggt ccgccgccgc atctgtgacg gcctggcgtt tcttgacatc
     1021 gccgtcgacg aagctcgcaa cgcggccaac aacccgatca tctcaccggc ggacagcaga
     1081 gtcgctgtcc acgtcatacc caccgatgag gagtcgatga tcgccggcgc cgccgctgag
     1141 ttcattcgtc gatctcagtc accttcaagc gttaatctgt gagctcatta acaacgcacc
     1201 actttctgag ctgtccgagg caattgacat caagggagga gccaaccgat ggagtcgagt
     1261 ctcaaaacgc aggtcccgcg tcagaagggg acaccgatcg cggacgccgc agtggaggcc
     1321 ccgctcgatc cggatgtcct ccgcaatatg gatgcttact ggcgagccgc caattacctg
     1381 tcggtcggcc agatttacct ttttgacaac ccactgttgc gcgagccgct caagctcgag
     1441 catgtcaagc cgcgattgct cggccactgg ggaaccactc cggggcagaa tttcatctac
     1501 gtccacctca atcgggtcat caagaaatat gacctgaatg tgatctacgt ctcggggccc
     1561 ggccacggcg ggccggcagt ggtcggcaac gtgtatctgg aggggacgta cagcgagatt
     1621 taccccaaca tctctgaaga cctctcgggg atgcagaagc tcttcaagca gttctctttc
     1681 ccaggcggca tccccagcca cgtcgcgcct gagacaccgg gctcgatcca cgaggggggc
     1741 gagctgggct acagcttgag ccactcattc ggcgcggcat tcgacaaccc ggacttgatt
     1801 gtcgcctgcg ttgtgggtga cggtgaggcc gagaccggtc cgctggccac gggatggcac
     1861 tccaacaagt tcctcaaccc ggcgcacgat ggggcggtgc tgccgatcct ccacttgaat
     1921 ggctacaaga tcgacaaccc gacgatcctt gctcgcatcg gccgagagga gctcgagcat
     1981 ctcttgctcg gctacggcta tacgccctac ttcgtcgagg ggcacgagcc ggaggcgatg
     2041 caccgcctga tggccacgac gctcgacaag gtcgtcgctg agatcaagcg gatccagtcg
     2101 gacgcgcgag agcggggtgt caccgagcgg ccgcgttggc cgatgatcgt cctcaagagt
     2161 cccaagggct ggaccggccc gaaggaagtg gatggccgac cggtcgaggg aacctaccgg
     2221 gcgcaccagg tcccgctttc cgagccgacc gagcaccccg agcatctcaa gatcctcgag
     2281 tcctggatgc gcagttaccg gccggaggag ctgttcgacg aaactggccg gttgatccct
     2341 gagttgcgtg agctcgctcc gaagggcgag cgtcgaatgg gcgccaaccc gcgcgccaac
     2401 ggcggcacgc tactgcggga tttgcgattg cccgactttt gcgactacgc gattgaggtg
     2461 ccaaggccgg gcaacgtcat gaacgaggat gcccgcaccc tgggcaagtt tctccgcgac
     2521 gtaatcaaac tcaacgccga gcaacgcaac ttccggatct tcggcccaga cgagaccgct
     2581 tccaaccgcc tgaccgccgt gttcgaggtg acgaacaagc aatggctcgg cacggtcgta
     2641 ccgaccgacg accgcctgtc ccctgacggc cgcgtcatcg agatgctcag cgagcaccag
     2701 tgcgagggct ggctcgaggg ctacctcctc acgggccggc acgggatttt caacacctat
     2761 gaggcgttcg tccacgtcat cgactcgatg ttcaaccagc acgcgaagtg gctcaaggtc
     2821 accagacgtc tcccgtggcg tcggccggtc gcctcgctga acatcctgct ggcctcgcac
     2881 gtctggcggc aggaccacaa cggtttcacc caccagtacc ccggtttcat cgaccatgtg
     2941 gtcaacaaga aggctgaagt gattcgggtt tacctcccgc ccgatacgaa ctgtctgctc
     3001 tcggtgatgg accactgcct gcgaagccgc cactacgtca atgtcgtggt ggcaagtaag
     3061 cacccgtctc cgcagtggct gacgatggac caggcggtca agcactgcac cgccggcatt
     3121 ggaatttggg agtgggccag caacgaccgc ggggctgagc ccgatgttgt catggcttgc
     3181 gcgggcgacg tgccgacctt ggagacgctg gccgccgtgc agcttctgcg cgagcacatc
     3241 ccggagttga agatccgcgt ggtcaacgtc gtcgacttga tgcggctcga gccggccacc
     3301 gagcatcccc acgggctgga cgaccacgac ttcgatacgc tttttaccaa ggacaagccg
     3361 atcgttttcg cctatcacgg ctacccatgg ctgatccacc ggctgaccta ccggcgcacg
     3421 aaccacaaaa accttcacgt gcgtggctac aaggaggaag ggacgatcag caccgcgttc
     3481 gacatgaccg tcatgaatga tctcgaccga tttcacttgg tggcggacgt tgtcgaccgc
     3541 ttgccccagc tcgggagcaa ggctgcgtac gccaagcaag ccgtacgcga caaactcatc
     3601 gagcatcggc agtacataac caagtacggc gaggacatgc ccgggatccg ggactggaag
     3661 tggacggtag caacacgtac gtgagcgccg cgacggatcc tagggggtga cgatcggcat
     3721 ggacgcccgc gacgaaagca acgaccgaca caatgagaac cgcgaaatga ggccggtcgc
     3781 gcaaccgacg ggtacggagt ccgcgcggta agcgaccggc aca
//
LOCUS       DATAQL010000935         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_706834, whole genome shotgun sequence.
ACCESSION   DATAQL010000935 DATAQL010000000
VERSION     DATAQL010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706834"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1504)
                     /locus_tag="VHS97_16265"
     CDS             complement(158..1504)
                     /locus_tag="VHS97_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3449810.1"
                     /translation="MRRRHYSSPDPTSTRVQYKTRDSPSCGDDLIDAEARVLTTDAGS
                     RLLAEIALVHSIRPSDIARFRQWAPPEVVSAAIRLSEARIKATAKFEHGQQMWVEPIG
                     IEQATSEAVARHKAARFTAPLVVDLCAGVGGDALALAAGSLVLAIDLDQGMCRRLRHN
                     ASVYRVADRILPVRSRAETFTIPAGAWLHLDPDRRTFKYRRARSLVDYAPGPEFWKSA
                     ASHSPAGAIKLGPASDFERHFGTSDHEIELISYRGECKEATAWFGELVTCRRRATRLP
                     ENVTWTDRDGPKSPSTPSSVLRSLIYDPDPALVRAGLLDGFAQAHGLARLADGAQYLT
                     SERMLLSPFVQAFHVLDVSPLDPRQLKRLITKHGIGTLEIKVRGVNVAPETLRRQLDL
                     NGDRSASLLLAGGTGPVRAVLAQRASTRGSITSSAGGAAGACDSASGVAPLPLSSD"
     gene            1532..>2314
                     /locus_tag="VHS97_16270"
     CDS             1532..>2314
                     /locus_tag="VHS97_16270"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEX3449811.1"
                     /translation="MLEYLSHSIPAVPPIVREPLLLGHVTIPTRFFLAPLAGYTSLAF
                     RLAVRDCGGLGLATTDLVNARSLIEGRRRAFELAETCEIDRPLSIQIYGIVIAEMEQA
                     ARLVAEQGASAVDINMGCPVRKVVKTGGGSALLCQVDRACELVAAVVKAVSIPVTVKM
                     RLGWDDESLTAPALARAFEEVGVAGVIVHGRTRQQGFSGTVNRAGIRAVVEAVDRIPI
                     IANGDIRTIADAALTFRETGCAAISIGRGALANPFIFQQLDCW"
BASE COUNT          432 a          686 c          769 g          427 t
ORIGIN      
        1 tcgaatggct tgacggcgtc aagcggcggt ttgccgctcg tccccgcccc gaagtccatg
       61 ttcagccaga tggggaagcc aaccgcgcgg ccccgctgga ggttaacggt cagtcggatg
      121 gcgaagccga tcgcgcgacc ccgctggagg tcaatggtca gtcggacgag aggggcaatg
      181 gtgcgacccc gctcgccgag tcacacgcgc ccgccgcgcc tccagccgat gacgtgatcg
      241 agccccgcgt cgaggctctc tgagcgagaa ccgctcgaac cggtcccgtg ccgccggcca
      301 gtaacaggct ggccgatctg tcaccgttta ggtctaattg ccgcctcaaa gtttccggcg
      361 cgacgttcac acctcgcacc ttgatttcga gcgtgccgat cccatgcttg gtgatcagcc
      421 ttttcaattg cctcgggtcg aggggagaca catccagcac atggaaagcc tgaacgaacg
      481 gggatagaag catacgctca ctggtcaggt attgggcacc gtcagcgagc cgtgccaggc
      541 cgtgtgcttg tgcaaagccg tccaataggc cggcacggac gagggcaggg tcaggatcgt
      601 aaatcaagct gcgaagcacg gaggaaggag tcgatggaga cttgggtcca tcgcggtcgg
      661 tccaggtgac gttctcgggg agccgggtgg ctcggcggcg gcaagtgacg agttcgccga
      721 accaagccgt cgcttcctta cactcgccgc ggtaactgat gagctcgatc tcgtggtcgg
      781 aagtgccgaa gtggcgctcg aagtcgctgg ccggcccgag tttgatggcg ccggccggcg
      841 agtgactggc ggccgacttc cagaactcag gaccaggtgc gtaatcaaca agtgatcgcg
      901 cacgccgata tttgaaggtt cggcgatccg ggtctaaatg aagccaggcg ccggcgggaa
      961 ttgtgaaggt ctcggcgcgt gaacggaccg gaaggattcg atcggcgacc ctgtaaacag
     1021 acgcattatg acggagtcga cggcacatgc cctgatcaag atcgatagcc agcacgaggg
     1081 atccagccgc aagagcaagg gcgtcgccgc cgacacccgc gcagagatcg acgaccagtg
     1141 gagccgtgaa tcgtgcggct ttgtggcgag cgaccgcttc ggacgtcgcc tgctcgatgc
     1201 cgatcggctc gacccacatc tgctggccgt gctcgaattt tgcggttgcc ttaatacgtg
     1261 cttcggacaa ccgaatcgcg gccgagacca cctcgggcgg cgcccattgg cgaaatcgag
     1321 cgatgtcaga agggcggatc gagtgaacaa gtgcgatctc ggccagcagc cggctgccag
     1381 cgtccgtggt caagacccgg gcctcggcgt cgatgaggtc gtcgccgcaa gagggtgaat
     1441 cgcgcgtttt atactggact cgtgtcgagg tgggatcggg actagaataa tgtcgtcgtc
     1501 gcatgcaatg acactcggcg aggagccaga cttgctcgaa tacctgtcac actcgatacc
     1561 cgctgttcca cccattgtgc gcgagccgct cttgttgggc cacgtcacga tccccacgcg
     1621 cttctttctg gcgcccctgg cgggttatac gagcctggcg ttccggttgg cggtgcgcga
     1681 ttgcggcggc cttggcctgg caacgaccga cctggtgaat gcccggtcgc tgatcgaagg
     1741 ccggcgccgg gcatttgaac tggccgagac ctgtgaaata gaccgcccgt tgtcgatcca
     1801 gatttatggg attgtcatcg ccgagatgga acaggcggcc cgattagtgg cagagcaggg
     1861 ggcttcggcc gtcgatatca acatgggttg cccggtcagg aaagtggtca agaccggagg
     1921 cgggtcggcg ctattgtgtc aagtcgatcg cgcttgcgaa ctcgttgccg cggtcgtcaa
     1981 ggctgtgagc attccggtga ctgtcaagat gcggctgggg tgggatgacg agtcactgac
     2041 ggcgccggct ttggcccgtg ccttcgagga agtgggcgtg gcgggtgtga tcgtccacgg
     2101 tcgcacgcgc caacaagggt tttcaggcac ggtcaatcgc gcgggcatcc gggccgtcgt
     2161 cgaggccgtt gaccgaattc cgatcatcgc caacggcgac atccgtacga tcgccgacgc
     2221 cgccctcacc tttcgagaaa ccggttgcgc ggccatctcg atcggccggg gcgccctggc
     2281 caaccccttt atctttcaac aactcgattg ctgg
//
LOCUS       DATAQL010000936         5228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4828550, whole genome shotgun sequence.
ACCESSION   DATAQL010000936 DATAQL010000000
VERSION     DATAQL010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4828550"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..375)
                     /locus_tag="VHS97_16275"
     CDS             complement(205..375)
                     /locus_tag="VHS97_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449812.1"
                     /translation="MSETKDISRRRLNDFLAARLRAKLVEQNNAEAPSAESIAVSSSP
                     RPDERATPVNES"
     gene            912..1700
                     /locus_tag="VHS97_16280"
     CDS             912..1700
                     /locus_tag="VHS97_16280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3449813.1"
                     /translation="MGFTRVPPAWRLPEGIDPPLWEYTHTSRLAAEEDAYFADHPLFR
                     VDERALHERFIDPGRLVDLGCGTGRHALRFAARGFSVTAVDLSRPMLQMVSAKAGEQG
                     IELTTVQSNLCRLGCFPDEVFDYALSMFSTLGMIRGRTSRRRALGEARRILRRGGRLA
                     LHVHNFWLNLRNPQGRSWLRTQAWQALFQRANLGDRRMTYRGIAGMKVHLYRWGELKC
                     ELRGAGFKIHEVLPLDEVSSASIGAPWFAHSLRAGGWIVFAVRR"
     gene            complement(1714..3237)
                     /locus_tag="VHS97_16285"
     CDS             complement(1714..3237)
                     /locus_tag="VHS97_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX3449814.1"
                     /translation="MTNERDWTRRRILKTAAAGSLVSALHCTDSRAQNKSTADCDRIP
                     RENELPGTRDWMTTKVAVDPKTRYRSPWIEGFASRTSVRPGESITLYVSTNPPSNFRV
                     QIYRLGYYQGHGGRLVMELAPIKGSVQPDPPIGHNRLRECAWAPCAEITIPSDWTSGV
                     YLGKLTAEREGMQSYVIFVVRDDRKCDFLFQVSDTTWNAYNRWPNQFSLYDDGKKEWY
                     WGPNVATSFDRPYGKYCQVVDAPLSVGSGEFLLWEFPLAYWLEQQGYDVSYISNLDTH
                     SDPASLLRAKGWLSVGHDEYWSLSMFETMKRMVSEGLNLAFLSGNSICGVIDIRASSD
                     GRSGRVIERVGRFGSPRKEEIEKGFPEEALFIKNGPNEATLMGAQSTYPVTGGGDWTC
                     RQPGHWLFAGTGMKEGDGIPGLVGWEWHGEPAPIPGLEVVASGKTQSPRAEGSYTATI
                     YPGPKENLVFNAATIWWADGLSAPPGYVRPAVYTRPQGPDPRVQRITRNLLERMRRS"
     gene            complement(3299..4150)
                     /locus_tag="VHS97_16290"
     CDS             complement(3299..4150)
                     /locus_tag="VHS97_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3449815.1"
                     /translation="MLPKLRFARLDAPIVLAHGLFGFTRIGLGRLTVTAYFRGIPSAM
                     EEAGNRVLVTRVPPIAGTEKRASRLADQIEHAFPDEPIHLIGHSMGGLDARRLLANAE
                     WQRRVLSLTTIGTPHLGTALADYAKLRVGRIYRLMASLGIDPQGCLDVTRRAARRFHR
                     LNPPPADIPCFSVAGDPIHETVCWPLQRTSAALWELEGPNDGLVSVESALAFGTPLPL
                     WPLDHFRQLNWLPHGESAVDLLPPIALYGQVVGHLASLGFGGETQPEVAQVQVDPPLL
                     AAGPAIG"
     gene            4365..4643
                     /locus_tag="VHS97_16295"
     CDS             4365..4643
                     /locus_tag="VHS97_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449816.1"
                     /translation="MRIHGIVRIATLEENPPGSDRIEMRIRVQGVRPDQPRLLVVPYE
                     LLLGDEALEPEQVQGKGFEAEVSEDESSRWVVSEIAFATGRVLRAPED"
     gene            complement(4731..>5228)
                     /locus_tag="VHS97_16300"
     CDS             complement(4731..>5228)
                     /locus_tag="VHS97_16300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3449817.1"
                     /translation="PLLLEAWAKVVCQSEANLILVGPGNDRQRLIELAASLGISNRVQ
                     FVGAVDSPAEYLRAADIFVLPSVAEGMSNSLLEAMATALPCVVSGIGGNTDLVADGQT
                     GRLVAQPTAEAWSNTIRELLENPSEARRLGSAAHQRIDREFALGVVVDRYLELYRRMI
                     AGTWP"
BASE COUNT          989 a         1546 c         1669 g         1024 t
ORIGIN      
        1 ccgccgactc gacttgccgg tggcaaatct ggtcgccgac gataccgtcc tgaccgaatg
       61 ctggggacgt gaagtcgctc cggttgagac ggggacgctc cggcgcttgg agctcgcgcc
      121 gagttcatcg cgggtgtacg caaccccaca ctaaccgcct ccaatgacag accggagcga
      181 gaccggcagg gacctgaacg gcttttaaga ttcattcaca ggcgtcgccc gttcgtccgg
      241 ccgcggcgac gatgagactg cgattgattc cgcgctgggc gcctcggcgt tattttgctc
      301 gaccagcttg gccctcagcc gggccgccaa aaagtcgttg agccgccgac gcgatatgtc
      361 cttagtttcg ctcattgcga atcatccttt cgcgattgga tcgtgcgcga gatatcgagg
      421 agtggccaat gcgcgtctgt ccgcggtgat cgagggagac gaagaataat aataatgcct
      481 caaccgcggc gaacggcgcg gagcgccacc catcagagac cttgctgcga cgaaaataag
      541 agtatttaga tcagaacaga atcgaattga cgaaccgtaa actggatcac gtaaaattct
      601 actcctccgc atgcccgtcg tccagaggct tcgtttttcc gcgagtggca gacgcaccgg
      661 gcggcgattg acgctgaaag agtacggctt gggacggacg aagtgacgcc ggtcacaagg
      721 gggcggtcca actgttgctg aaaccgggac gcgcgagatg gtcgctttgg gattctgggc
      781 ggtttgtggc acgtcgagtc gaatcgacga ttcatattgg cgatcttgag gttatattga
      841 ttggtcgacg agtcgaccac acaattttga cgcaatgacc tgccgccttt gtaacgcaac
      901 cggtcgttct tatgggcttc acacgagttc cgcccgcatg gcgcctgcct gagggtatcg
      961 atccgccact atgggaatac acacacacct cgcgattggc cgccgaggaa gacgcttatt
     1021 ttgccgatca tcctttgttt cgggtggacg agcgtgctct ccacgaacgg ttcatcgatc
     1081 cggggcggct ggtggatctg ggctgtggaa cggggcgaca cgcgctccgc tttgccgcac
     1141 gcggattctc ggtgaccgcc gtcgatctgt cgcggcccat gcttcagatg gttagcgcaa
     1201 aggcaggcga gcaagggatc gagctgacga cggtgcagtc taacctgtgc cggctgggct
     1261 gttttcccga cgaggtcttt gactatgccc tgtcaatgtt cagcacgctg ggaatgatcc
     1321 gcggccgcac gtcgcggcgg cgagcactag gggaagcccg gcgcatcctc cggcgcggtg
     1381 ggcgattggc gctccacgtc cataatttct ggctcaatct tcggaacccg caaggccgaa
     1441 gctggttgcg cactcaagcc tggcaagctc tcttccagcg ggccaatctg ggtgaccgcc
     1501 ggatgaccta ccgcggaatc gcgggcatga aggttcatct ttatcgctgg ggcgagctga
     1561 aatgtgagct gcgcggggcg ggtttcaaga tccatgaagt cttgccgctc gacgaggtct
     1621 cgtcagcgtc gatcggagct ccttggtttg cgcacagtct ccgcgcggga gggtggatcg
     1681 tcttcgcggt gaggcggtga ccgggatgat tgctcacgat cggcgcatcc gttccagcaa
     1741 attccgcgtg attcgctgta cgcgcggatc tggcccttgc gggcgcgtat aaaccgccgg
     1801 gcgaacataa ccgggagggg cggagagccc gtcggcccac cagattgtgg ccgcgttgaa
     1861 gacgaggttt tctttggggc cggggtaaat cgtggctgta tacgatcctt cggcacgcgg
     1921 gctctgagtt ttgccgctgg cgacgacttc cagtcccggg attggggcgg gctcgccatg
     1981 ccactcccag cccaccaggc cgggtatgcc gtccccttct ttcatgcccg tgccggcgaa
     2041 cagccagtgg ccgggctggc ggcaggtcca gtcgccgcca ccggtcacgg gataggtgct
     2101 ctgcgcaccc atcagtgttg cttcatttgg accgtttttg atgaagagag cctcttcagg
     2161 gaaacccttc tcgatctctt cctttcgcgg cgaaccgaac cgtcctaccc gttcgatgac
     2221 gcggccggag cggccatcgc tcgacgcgcg gatgtcgatc acgccgcaga tgctgttgcc
     2281 cgatagaaac gcgagattta aaccctccga gaccattcgt ttcatcgtct cgaacatcga
     2341 tagcgaccag tattcgtcgt ggcccactga cagccagccc ttggcccgca gcaggctggc
     2401 cgggtcggaa tgagtatcga ggttggagat gtagctcacg tcgtaaccct gctgctccaa
     2461 ccagtaggcc agcggaaatt cccacagcaa gaattcgccg gagccgaccg acaagggcgc
     2521 atcaacgacc tggcaatact tgccgtaggg ccggtcgaag ctcgtcgcga cattcggccc
     2581 ccagtaccac tccttcttgc cgtcgtcata gagtgaaaat tggttgggcc aacggttata
     2641 ggcgttccag gttgtgtcgg agacttgaaa gagaaagtcg catttacggt cgtcccggac
     2701 cacgaagatg acatagcttt gcattccttc gcgctcggcc gtgagctttc ccaggtaaac
     2761 accgctggtc cagtcggagg ggattgtgat ctccgcgcag ggagcccaag cacattcgcg
     2821 cagccgattg tggccgatgg gtggatcagg ctggacagag cccttgatcg gagcgagctc
     2881 catgaccagt cgaccgccgt gtccctggta ataaccaagc cgatagatct gcacccggaa
     2941 gttcgacggc gggttcgtgc tgacgtacaa agttatcgat tccccaggcc gtacgctcgt
     3001 gcgcgacgcg aagccttcga tccatgggct gcggtacctg gtcttgggat cgacggcgac
     3061 cttggttgtc atccagtcgc gcgtgccggg tagctcgttc tcgcgtggga tgcgatcgca
     3121 gtcggccgtg gacttgtttt gggcccgaga gtctgtgcaa tggagtgccg aaaccaggct
     3181 gccggcggcg gcggtcttga gaatgcggcg gcgggtccag tcgcgttcat ttgtcatgct
     3241 gagcgagctc ccccagtggc cggacaaggt tcggttggct gctggcgtca gctttagttc
     3301 aaccgatcgc cggccccgcg gcaagaagcg gcgggtcaac ctgaacttgc gcgacctcgg
     3361 gctgggtttc gccgccgaaa ccgagagagg cgagatggcc cacgacttgg ccgtacagtg
     3421 cgatcggtgg cagcaggtca acggcactct cgccgtgagg cagccagttc aactgccgaa
     3481 agtgatcgag gggccacagc ggtaaggggg tgccgaaggc gagggccgac tcgactgaga
     3541 ccaggccgtc gttgggccct tccagctccc agagcgcggc cgaagttcgt tgcagaggcc
     3601 agcagacggt ttcgtgtatc gggtcgccgg ccacgctgaa acaaggaata tcggcaggcg
     3661 gcggattgag gcgatgaaac cgccgagctg cgcggcgcgt cacgtcgagg cagccctgcg
     3721 gatcaattcc caacgaggcc atcagccggt agattcgccc gactcgcagc ttggcatagt
     3781 cggcgagcgc cgtcccgagg tgcggcgttc cgatggtcgt caggctgaga actcgccgct
     3841 gccattccgc attggccagc aaacggcgcg catcgagtcc gcccatgcta tgcccgatca
     3901 agtggatcgg ttcatcgggg aacgcatgtt cgatctggtc ggcgagccga ctggcccgtt
     3961 tctcggtgcc ggcaattggc ggcacgcggg tcaccagtac gcggtttccc gcctcttcca
     4021 tcgcactagg aattccgcgg aaataggccg tcaccgtcaa gcgacctaag ccgatgcgcg
     4081 tgaacccgaa cagaccgtga gccagcacga tcggcgcgtc caaacgagca aaccgcaact
     4141 tgggcaacat cggcgtcgca acaccttgat cggcctcgcg cggcaagagc tccgacggcg
     4201 acacgaggac aatggagtct ggataccagt ctcttgcttg acaaactctt gactcacgtg
     4261 ccattgtgca cgatgaatcg ggcccgccgc atgatcgacc tttctctttt gcgttccggt
     4321 cgtgccggtc gatccgatcg tcggattgaa gcaggaggtc gaccatgcgg attcacggga
     4381 ttgtacgcat tgcgacgctg gaagagaatc cgcccggatc agaccggata gaaatgcgaa
     4441 ttcgggtcca gggagtgaga cccgatcagc cgcgactgct cgtggtccca tacgaactct
     4501 tgctggggga cgaggcgctc gagcctgagc aggtgcaggg aaaaggattc gaggcggagg
     4561 ttagcgaaga cgagtccagt cgctgggtcg tcagcgaaat cgcgtttgcc acgggccgag
     4621 tactacgtgc tccagaagac tgaattcact cagctaaact ggttggtgat ctttaccggc
     4681 tcgcctttgg cgtcgagcaa tgacgggctg aagcctgcat ttcccaagcc tcacggccag
     4741 gtcccggcga tcatccggcg atagagctcg agataacgat cgacgacgac tccgagtgca
     4801 aattcccggt cgatccgctg gtgggctgcg ctgccgagcc ggcgggcttc gcttgggttt
     4861 tcgagcagct cgcggatggt gttggaccag gcttcggccg tgggctgggc gacaaggcga
     4921 cccgtctgac catcggcgac caagtcggtg ttgccgccga tccccgacac cacgcatggc
     4981 agggcggtcg ccatggcttc cagcaacgag ttgctcatcc cctcagcgac gctgggcaag
     5041 acgaagatgt cggcggcgcg caggtattcg gcgggactgt ccacggcccc gacaaactga
     5101 acgcggttgg aaattcctag cgacgccgcc agctcgatca gccgctggcg atcgttgccg
     5161 ggcccgacca ggatcaggtt ggcctcgctc tggcagacaa ctttggccca cgcttcgagc
     5221 aagagcgg
//
LOCUS       DATAQL010000937         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4523108, whole genome shotgun sequence.
ACCESSION   DATAQL010000937 DATAQL010000000
VERSION     DATAQL010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4523108"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /gene="ybeY"
                     /locus_tag="VHS97_16305"
     CDS             <1..614
                     /gene="ybeY"
                     /locus_tag="VHS97_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX3449818.1"
                     /translation="EPRSIDRETAETFKRALRFGLSHMGALIEVEISNTQGHLRVDRA
                     ALEQLVCVVLAAEDRARASISIALVDNASIHAVNQTYLGHDWPTDVISFPLSAPADPV
                     LTGELVISAEMACTCAREIGVDPRHELALYVVHGLLHLCGFDDHDHADRRRMRDREDV
                     LLSQAGLTNPYPLGNEALPGLHDHHAMGSRRLTRKLEGQPWTA"
     gene            602..1915
                     /locus_tag="VHS97_16310"
     CDS             602..1915
                     /locus_tag="VHS97_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX3449819.1"
                     /translation="MDGMSLPWILVVGLPVLALHLVSMALTKAIQSYSRGLLEDRCDD
                     RGRPERAQEVEHWDHKTERAAEALAVLTGLLLAALMGAGVGLASFPPRVVVVIVLVLV
                     IGLLGYVIAGVVGKVFAEVIIDLLWPLAGSLRAAALPLTFGLRQVGHLVESLSGRSES
                     LHRPAHLQVEIPVEDDAPDEDAEPEIPESARVLLQQAVILTRTDVGELMTPRSSIISL
                     PSTVSAAEAAASFRRTGLSRIPIFEESRDDIVGVLYAKDLFARMTETRTPAAISPREL
                     IRPALFVPESKNAYDLLEELRTQRTHFAVALDEYGSVAGVVTLEDLLEELVGPIDDEH
                     DIPSPADPVRELGGSRYDVDATLSVDELNLRLGVQLPTDGEFQTIGGLVFHELGRLPA
                     RGDSGNAFGMAFTVIEVSDHTIRRLAIDLAPESATVEREPSKSSK"
BASE COUNT          356 a          636 c          658 g          419 t
ORIGIN      
        1 acgagcctcg ctcgatcgat cgagaaacag cggaaacttt caagcgggcg ctccgattcg
       61 gactatcaca catgggcgcg cttatcgaag tcgaaatcag taatacacaa ggccatctgc
      121 gcgtcgaccg cgccgctctc gaacagctcg tatgcgtcgt gcttgccgcg gaggatcgcg
      181 cgcgcgcttc gatatcgata gcgctcgtcg ataacgcgtc gatccacgcg gtcaatcaga
      241 catatctggg ccacgattgg ccgacggacg tcataagttt ccccttgagt gcacccgccg
      301 acccggttct gacgggcgag ctggtgatct ccgccgagat ggcgtgcact tgcgcgcggg
      361 agatcggcgt cgatccccgg catgagctcg ccctttacgt ggtccatggg ctcttgcatc
      421 tctgcggctt tgacgaccat gaccatgccg atagacgacg aatgcgcgac cgcgaggatg
      481 tgctgctctc tcaggcaggg ctgactaacc cgtaccccct ggggaacgag gctttacccg
      541 ggctgcatga ccaccacgct atgggatctc gtcggttgac ccgtaagctg gagggccagc
      601 catggacggc atgagcctgc cctggattct ggtggtgggg ttgccggtgc tggcgttgca
      661 tcttgtgtcg atggcgctca ccaaggcgat tcagtcgtat tcgcgcggcc tgctcgaaga
      721 ccggtgcgac gaccgaggcc gccccgaacg cgcccaagaa gtcgaacatt gggaccataa
      781 gacagagcga gcggccgagg cattggccgt tctgacgggc ttgctgctgg ctgcgctcat
      841 gggagcgggc gtgggactgg cgtcctttcc tcctcgcgtg gtggtcgtca tcgtgctggt
      901 cctcgtgatc ggccttttgg gctacgtgat tgccggcgtc gtcggcaagg tgtttgccga
      961 ggtcatcatc gatctgctct ggccgctggc gggttcgctc cgcgccgccg ccctgcccct
     1021 gacctttggt ctgcgccagg tggggcacct ggtggaatcg ctgtccggcc gttcggagtc
     1081 gctgcatcgt ccagcccact tgcaggtcga aattcccgtt gaggacgatg cgcccgacga
     1141 agacgccgaa cccgaaatac ccgagtctgc ccgcgtcctc ctgcagcagg cggtcatcct
     1201 gactcgcacg gatgttggtg agttgatgac gccgcggtcg tcgatcattt cgctcccgtc
     1261 gaccgtctct gccgcggagg ccgccgcgag cttccggcga accggtctgt cgaggatccc
     1321 cattttcgag gaaagccgcg acgatatcgt gggcgtgctc tacgccaagg atttgttcgc
     1381 acggatgacc gaaactcgga cgccggccgc gatctcccca cgcgagctga ttcggccggc
     1441 gctcttcgtc cctgagagca aaaacgctta cgatctcctc gaagagttgc gaacccaacg
     1501 gacacacttt gccgtcgctc ttgacgaata tgggagcgtg gcgggcgtgg tcactctgga
     1561 ggacctgctc gaagaactgg tcggtcccat cgacgacgag cacgacatcc catctccggc
     1621 cgaccccgtc cgtgagcttg gtggttctcg gtacgatgtc gatgcgacct tgtcggtcga
     1681 tgagctcaac ctacgcctgg gcgttcaatt accgacggac ggggagtttc agacaatcgg
     1741 cggtctggtg tttcacgagc tgggccggct gccggcgagg ggcgattctg ggaatgcctt
     1801 tggaatggcg ttcaccgtga tcgaggtctc ggatcataca atccggcggc tggcgatcga
     1861 cctcgcacct gaaagcgcga cggtcgagcg cgagccttcc aagtccagca agtaaccgcg
     1921 gcctagtgag ccgcggcttg atcgaccttc ccctggcttg agagaacgac tccaagattg
     1981 gtgtgtgtgt cgacgcggcc ggaattaagc cgaagtgcct cgcggtaatg agcctcggcc
     2041 tcggggagtt tcccctgaac agagaagtc
//
LOCUS       DATAQL010000938         5648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4523201, whole genome shotgun sequence.
ACCESSION   DATAQL010000938 DATAQL010000000
VERSION     DATAQL010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4523201"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..829
                     /locus_tag="VHS97_16315"
     CDS             329..829
                     /locus_tag="VHS97_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449820.1"
                     /translation="MLEIPDDSGFLALIVPGMYASFVAETWDFELLRSHFRRQMARHS
                     MLIWGTGLEGMWRVDVQVDGPDVRGFREVVGPLHVEGRSVLVTSYDSLTMAAQFPDVS
                     LPQAHERDQLVPLPDGDYICRVVQLFDPNACESAGGESRDFVIILRASTRPVQKWSEI
                     PWCSVP"
     gene            complement(863..2113)
                     /locus_tag="VHS97_16320"
     CDS             complement(863..2113)
                     /locus_tag="VHS97_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX3449821.1"
                     /translation="MAPIKNVDISSPAFKANPYPFYAQLRDQAPVHRIILGDKRPAWL
                     VTRYDDVVTVLKDDRFVKDKRKVMTPEQMAREPWIPKMIQPLERNMLDADAPEHTRLR
                     GLVHMAFTPRLVENMRERIQRLTDELLNPIETRGRMDLIRDYALPVPTTIIAEMLGVP
                     ERERHKFHRWSNTIVSIVPSKWALLKAIPSLIAFLRYIRRLVRTRRAAPRDDLVSALV
                     ESHEAGDRLSEDELLAMVSLLLIAGHETTVNLIGNGMLALLYHPQQLERLRDDPGLIK
                     PAVEELLRYDGPLETATERYAREDATIAGVTIPRGEMVFAVLASANRDERQFDHADTL
                     DITREPNRHLAFGQGVHFCLGAPLARLEGQIAINTLLRRIPDLRLDVPRHALRRRPGL
                     VLHGLKSLPVAFSAVREPVESARL"
     gene            2620..3099
                     /locus_tag="VHS97_16325"
     CDS             2620..3099
                     /locus_tag="VHS97_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3449822.1"
                     /translation="MAIESWDPFREAVSLRDAMNSLFQESFVRPGGMSAPNVHAALPL
                     DVSETEDAFVVKASLPGVKPDDVQITVQGDTLTIRGESKAEEDKDGEHWHLRERRFGS
                     FQRSVSLPMPVNSDQSEARYEHGVLTLTLPKSEAAKPRQIKIKGASQSNVGIGNGPK"
     gene            3452..4543
                     /locus_tag="VHS97_16330"
     CDS             3452..4543
                     /locus_tag="VHS97_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449823.1"
                     /translation="MSQKRPGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRIQCT
                     NNLKQLGLGLANYESSNTSYPIALVYGVGVGLCKSPGFGRGCQNTPWFVLMLPYIEQG
                     PLYNSFNASIGMEGPPLGSAPLGGFVVNSTVFTTKIPSFQCPSDNANTFSFATLNAAT
                     GGQVPAYAFSPTKGNYGINVGNTDYGGIASGLFPTLYRQAPFASNTSGSGPLTIRVAS
                     VTDGLSNSQFVSEILQGANDDTRGTVWFVDPGSGSYMTRFTPNGNQDFLGSGVNVDVV
                     ASFVTGVGHSPPHAGELCDSQPAQMLACFNSSSEGAAFGGSRSRHPGGVNTLFGDGSV
                     HFMKNSINPQTWIALGTISGGEVLSSDSY"
     gene            complement(4590..>5648)
                     /locus_tag="VHS97_16335"
     CDS             complement(4590..>5648)
                     /locus_tag="VHS97_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3449824.1"
                     /translation="GMGLEWAKAVSQTEPIRLELLGTTEYDSDNQTRIRPLFRGRVDR
                     VYAKVGQLIAKGEPLIEFYSTDLAEAKNACEIERIQWVYSSNLLKVRENLSKTNAISR
                     QSYLEAQNDEMKNLREYEVARDKLLVYGLTEDEVAKVKDEVGSQKARMTFRSPASGIV
                     IFRDVVPGNVYDDDDTLLVIEPLDHLWVWGNVFESDLDLIKLGQSWEIQFPFQAERLH
                     GEVEYISNNVDPGTHAVRVRTSIANSEGRIKSDMLVRGSLEIPPDHNRTVVTRSALVV
                     NASKFYVYVKLPGSALQYRRVPVAVAQEKDDHVVIESGLKSGDEVVTVGALILDQIFD
                     NARVTRTEEVANASPGVN"
BASE COUNT         1126 a         1634 c         1661 g         1227 t
ORIGIN      
        1 atcattgtgc cggctcggat ccaactggca agagattgac gcttcttgat acgatctaag
       61 tggaatcaag tcacagagtg gaaggcgttt tccacaaccg gcaaatgcgt aagtcgtcgg
      121 ccaaggacct cgcgatgcag cctctcttag ccgacgcgga tttcgccgag atcctgatcg
      181 cagatcgagc aatcgttttc attgacttcg cgtggtccgg acaatcgagg ctctctgccg
      241 ccgtcatcga agagtgggag cgaacgtcga gcatccgagt ccgccaccat cggatctctg
      301 ggaccgcgaa ttggatggtt gaaactacat gctcgaaatc cccgacgatt cagggttcct
      361 ggcgctgatt gtgccgggaa tgtacgcatc ctttgtggct gagacctggg acttcgagct
      421 tctacggtcg cacttccgac ggcaaatggc ccggcactcg atgctcattt ggggcaccgg
      481 actggaaggg atgtggcgtg ttgacgtaca ggttgatggt cccgatgtcc ggggcttccg
      541 cgaagtcgtc ggtccccttc atgttgaggg ccggtcggtg ctggttacca gctacgacag
      601 tctcacgatg gcggctcagt tccctgacgt gtcgctgcct caggcccacg agcgcgatca
      661 attggtcccg ctaccggacg gcgactacat ctgtcgagtg gtccagttgt tcgatccgaa
      721 cgcttgcgaa tcggctggag gggagtccag ggactttgtg atcatactta gggcatccac
      781 gcgaccggtg caaaagtggt ctgagattcc ctggtgttca gtcccctagc ctcgagccgt
      841 cgctgcccag gcacgccgag ggctacaacc gagcactttc gactggctcg cgcacggctg
      901 aaaacgccac gggcagagat ttcaggccgt gcagaaccaa tcccggcctc cgcctcaggg
      961 cgtgccgcgg cacgtccagc cgcaggtcgg gaatccggcg cagcagagtg ttgatggcga
     1021 tctgcccctc cagacgggcc agcggtgcgc ccaagcagaa atgaacgcct tggccgaacg
     1081 ccaggtggcg gttcggctct cgagtgatgt ccaaagtatc tgcatgatcg aactgccgct
     1141 cgtcccggtt ggcggatgcc agcacggcaa acaccatctc accgcgcgga atcgtgactc
     1201 cggcgatcgt cgcgtcctcg cgcgcgtacc gttccgtcgc cgtttcgagc gggccgtcat
     1261 agcgcaagag ctcttcgacc gccggcttga tcagcccggg gtcgtctcgc agccgctcta
     1321 gctgctgcgg atgatacagc aaggccagca tcccattgcc gatcaggttg accgttgtct
     1381 catgtcccgc aatcagcagc agggagacca tcgccagcag ttcgtcctca ctcaaccggt
     1441 cgccggcttc gtgtgattcc accagggcgc tcaccaggtc atctcgggga gcggcgcgcc
     1501 gggttcgaac gagccttctt atgtaacgca agaacgcaat caacgacggg atcgctttca
     1561 gcaacgccca cttgctcggc acgatcgaaa cgatggtgtt tgaccagcgg tgaaacttat
     1621 ggcgttctcg ctccggcaca cccagcattt ccgcaatgat ggtcgtgggt actggcagcg
     1681 cgtagtcccg aatcaagtcc atgcgtccgc gggtctcgat cggattgagc agctcgtcgg
     1741 tcagtcgctg gatgcgctcg cgcatgttct cgacgagccg tggcgtgaac gccatgtgga
     1801 ctagcccgcg cagccgggta tgttcgggcg catccgcgtc aagcatgttg cgctcgagcg
     1861 gctgaatcat tttcgggatc cagggctctc tcgccatttg ttcgggcgtc atgaccttcc
     1921 gtttgtcttt gacgaagcgg tcatccttga gcacggtgac cacgtcgtcg tagcgagtca
     1981 ccagccaggc aggccgtttg tcaccgagga tgatgcggtg aaccggcgcc tggtcgcgta
     2041 actgcgcgta aaacggatac gggttagcct taaaggccgg gctactgatg tcgacattct
     2101 tgatcggcgc catgaacgtc ttcctatcaa cgacgagagc ggacggaatg gacaccaccg
     2161 acgataccgg aggcaactgc cgaattcgac gggttagccg acgttgcatt tgtgcctgca
     2221 gtggtcacgt gagaggggaa gaggcagagc ggctggtcgc cccaacgagg cactgtcatt
     2281 tttgcgaccg catcggaaat ggcctcctgt catcttggca ggaatttagc ggtgtccctt
     2341 ggaataaatc tcctaaatac gcttagtaaa aaggtttaca gtggatgtaa ttaatcggca
     2401 taactgttgc aagtgatgga taaccgggag tggctttcag gacggcgttc cacgggtcgc
     2461 tgctccgagc catctcgtgt gatgccggca cccttcacgc ggtgggggtg tttttcgggt
     2521 tctatcaact ctttttgcgg gctcgtctga cggatcgaac ttggtaatag cgaaggcggc
     2581 tctgcagcct tcggcattga tcagaaagag aggctcacca tggcgatcga aagttgggac
     2641 ccattccgtg aggccgtcag cctccgcgac gcgatgaatt cgctgttcca ggagagcttc
     2701 gtccgaccgg gcggcatgtc ggctccaaac gttcatgccg cgctgccgct cgacgtgtcc
     2761 gagacggagg acgcgttcgt ggtgaaggcg tcgctgcccg gggtcaagcc agacgatgtt
     2821 cagattaccg tccaggggga cacgctgacg attcgcggcg agagcaaggc cgaagaggac
     2881 aaggacggtg agcactggca cctccgcgag cggcggtttg gctcgtttca gcggtcggtg
     2941 tcgctgccca tgccggtcaa ctcggaccag tcggaggccc gatacgagca cggcgtgctc
     3001 acgctgacgt tgcccaaatc ggaagccgcc aagccccggc aaattaagat caagggggcg
     3061 agccagtcca acgttgggat tggcaacggc cccaagtgag gccgtcctct tcccggaggg
     3121 tgcgccagct cccgaagaga atgacaacag tctcaagatc tgcatctcaa gcggccgggg
     3181 tgagtaaccc cggccgcttt ctcgttaaac cgctcttcga tcacggcagg tcaggaggac
     3241 cgcagggcac catcgtgggt caaggcccga aacgcgttct tcttctgaaa taattcacag
     3301 atccgaaaaa tagaaggcgt tatactcgcc gcgacgaaga tcaatgcctt ttagttcgcc
     3361 tgatcatcgt cctgtctttt taaactcgaa tagtgtaatt tcgttcgtgt tgatgttttc
     3421 ctgatcacga taacggaggc acgttaaagg aatgtcacag aaacgtccgg gctttacgct
     3481 gatcgaactc ctcgttgtga tcgcaatcat tgcagtactc atcgccttgc tactgccggc
     3541 cgtgcaggca gcacgcgaag cagcgagacg gattcagtgc accaacaatc tcaaacagtt
     3601 ggggctgggt cttgccaatt atgagtcgtc gaatacctcg tatcccattg ctctggtcta
     3661 tggtgtcggg gtcggcctct gcaagagccc cggcttcggc cgaggatgcc aaaacacgcc
     3721 ttggtttgtt ctgatgctcc cttatattga gcaggggccg ctctacaact ctttcaacgc
     3781 gtctatcggt atggaaggcc ctcctctggg atcagcccct ctggggggat tcgtcgtcaa
     3841 cagcacggta ttcacgacta agatcccctc attccagtgc ccaagcgata acgcgaacac
     3901 ctttagcttc gcaaccttga acgcggcgac cgggggtcaa gttccggcgt acgctttctc
     3961 gccgaccaaa ggaaattacg gcatcaatgt gggtaataca gattacggcg ggatcgcttc
     4021 cggtctgttc ccgacgctct atcgccaggc cccgtttgcg agtaacacga gcgggtcggg
     4081 tccgcttaca atcagggttg catcggtaac agacgggctg agcaatagcc aattcgtctc
     4141 agagatcttg cagggagcta acgacgacac ccgaggcact gtctggttcg tcgatcccgg
     4201 ctcgggctcc tacatgacgc gattcacgcc caacggcaat caggatttct tggggagcgg
     4261 tgtgaacgtg gacgtcgtcg ctagcttcgt gaccggtgtg ggccacagtc cgccacacgc
     4321 cggcgagctt tgcgacagcc agcctgcgca aatgctggcc tgctttaact cgtcatcgga
     4381 aggagctgcc ttcggaggct ctcgaagccg ccatcccggc ggcgtcaata cccttttcgg
     4441 tgacggctcg gtccatttca tgaagaactc gatcaacccg caaacgtgga tcgcacttgg
     4501 tacgatcagc ggtggcgagg tactcagttc cgattcatat taagaagggg acgttcgaga
     4561 aactgggaaa gatgaagtga caggtggcct taattcactc ccggcgaggc gttggcaact
     4621 tcctcggtcc gggtgactcg tgcgttatcg aagatctggt cgaggatcag ggcaccaacc
     4681 gtcacgactt cgtcaccgct cttcaaaccc gattcgatga cgacatggtc gtctttctct
     4741 tgggcaacgg ccaccggaac gcgccggtat tgaagtgccg acccgggcag cttgacgtag
     4801 acgtaaaact tgctcgcgtt gacgaccagc gcgctgcggg tgaccacggt gcggttgtga
     4861 tcgggcggaa tttccaagga tcctcgcacc agcatgtcgg acttgattcg gccttcgctg
     4921 ttggcgatcg aggtgcgcac cctcacggca tgcgttcccg ggtcgacgtt gttggagatg
     4981 tactccacct cgccatgcag ccgctccgcc tggaacggga actgaatttc ccatgattgg
     5041 cccagcttga tcaggtcgag gtcgctctca aaaacgttgc cccacaccca gaggtgatcg
     5101 agcggttcga tcactagcag tgtgtcatcg tcgtcgtaaa cattgccggg gacgacgtcc
     5161 cgaaagatca cgatcccact tgccggcgag cgaaaggtca ttctggcttt ctgcgagccc
     5221 acctcatcct tgaccttggc gacctcgtct tccgtcaagc cgtagaccaa aagcttgtca
     5281 cgcgccacct cgtattctcg gaggtttttc atctcatcat tttgtgcttc caggtaagac
     5341 tgacgggaga tggcattggt cttggacagg ttttctctaa ccttgaggag gttactggaa
     5401 tagacccatt gaattcgctc gatctcgcag gcgttcttgg cctcggccag atcggtgctg
     5461 tagaattcga tgagcggttc tcccttcgcg ataagctggc ctaccttcgc ataaacgcga
     5521 tccactcgcc ccctgaaaag cggacgaatc cttgtctgat tgtcggagtc atattcggtt
     5581 gtgccgagca gctcgaggcg aatcggttct gtctgggaaa cggccttggc ccactccaaa
     5641 cccatccc
//
LOCUS       DATAQL010000939         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3609126, whole genome shotgun sequence.
ACCESSION   DATAQL010000939 DATAQL010000000
VERSION     DATAQL010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609126"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VHS97_16340"
     CDS             complement(<1..388)
                     /locus_tag="VHS97_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449825.1"
                     /translation="MRPHREPLPARRQVPEPHQPVLAARRQGPAVGRERQAPGPAKFE
                     PRDRPLSTGGQIPDRDGPLARRGQRLAVAAEGEGARPPVVALEGAQRPFRVGLPDRDG
                     REIPDRQRRAVGRERRGEARDGGAAAR"
     gene            complement(586..>1800)
                     /locus_tag="VHS97_16345"
     CDS             complement(586..>1800)
                     /locus_tag="VHS97_16345"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX3449826.1"
                     /translation="GTLFGLDYDLLLYDLTSTYFEGLAEENDLARRGYSRDHRSDCKQ
                     IVLALVVTRDGFPLAHFTLAGNTQDLQTVETIVTTIETRFGKSHRVWVMDRGMISAET
                     LAFLSRGGRRYLLATRRGELASFAEHLRTGGWQRLEDNPDVAVKLLKRNRVHYLLARS
                     RPRRQKERAIRRRQRRGLARALGRLQRRIESGRLKSRDKILESVGRLKGRFPKARSFV
                     TITVTKAAPARLHWTWERGKFRAALAGDGAYLLRSNQGGWSAEEFWQTYIQLTVVEHA
                     FRVLKSELLLRPVWHHYSGRTQAHVMICVLAYALWKTLDHLAKRAGLKTEIRKPDPTR
                     PKASAKSRTMTPEVILRELAQLAIGDIELETTDGQRLTLRRVARPKGEQKRILETLKL
                     DVPERLSPDRIL"
BASE COUNT          263 a          617 c          611 g          309 t
ORIGIN      
        1 ctcgcgctgc ggccccgcca tcccgggctt cgcctcggcg ttctcgcccg acggcgcgac
       61 gctggcggtc ggggatctct cgaccgtcac gatccgggag gccgacgcgg aaaggccgct
      121 gcgcaccttc gagagcgacg acgggtggcc ttgcgccctc gccttcggcc gcgacggcaa
      181 gtcgctggcc gcggcgggcg agcgggccgt ctcggtctgg gatctggccc ccggtcgaga
      241 gggggcggtc acgaggctcg aacttcgcgg ggccaggagc ctggcgttcg cgcccaacgg
      301 ccggtccctg gcgacgggcg gcgaggacgg gctggtgcgg ctctgggacc tggcgacggg
      361 ccggcagcgg ctcgcggtgc gggcgcacgc ccgttccgcg accgcgctgg cgttctccga
      421 cgacggccgg gcgctggcct cggcctcctc ctgtgagcgt gtcgcccggc tctgggacgc
      481 ggcgacgggc cgcgggctcg gggcgcttag cctaagttgc gcagttggcg actccgaacc
      541 tcggatttcc ccggaaatca cccctcctgg gttgccaggt cttcactaca aaatccggtc
      601 gggactcagg cgctcgggga catcgagttt cagggtctcc aggatccgtt tctgctcccc
      661 cttcggacga gccacgcggc gaagggtcag cctctggccg tcggtcgtct ccagctcgat
      721 gtcgccgatg gcgagttggg ccagctctcg gaggatgacc tcgggtgtca tcgtacggga
      781 tttcgctgaa gccttgggcc gggtgggatc gggcttgcga atctccgtct tcaggccagc
      841 ccgcttggcc agatggtcga gtgtcttcca gagtgcatac gccagcacgc agatcatcac
      901 gtgcgcctga gtccggccac tgtagtgatg ccacaccgga cgcagcagca actcgctctt
      961 gagcacacgg aaggcatgct ccaccacggt caactggatg tacgtctgcc agaactcctc
     1021 ggcggaccat cccccttgat tgctccgcaa caggtacgcg ccgtcgccgg ccaaggccgc
     1081 ccggaacttc ccccgctccc aggtccagtg cagccgcgcc ggcgcggcct tcgtcacggt
     1141 gatcgtcacg aacgagcgcg ccttggggaa acggcccttg agtcgaccga cactttcgag
     1201 gatcttgtcg cggctcttga gtcgaccgct ctcgatccgc ctctggagcc tccccaaggc
     1261 acgtgccagc ccgcggcgtt ggcggcggcg gatggcacgc tccttctgac gccgtggccg
     1321 actccgtgcc aggagatagt gcacacggtt gcgcttgagc aacttcaccg cgacatcggg
     1381 gttgtcctcc agacgctgcc agccgccggt gcgcaagtgc tctgcgaacg aggccagctc
     1441 accgcgccgc gtagccagga gataccgccg gccacctcgg ctgagaaacg ccagtgtctc
     1501 cgcgctgatc atgccgcggt ccatgaccca gacccgatgc gatttcccga accgcgtctc
     1561 gatcgtggta acgatggtct ccaccgtctg gaggtcttgg gtgttgcccg cgagggtgaa
     1621 gtgggccagg ggaaaaccgt cacgagtcac gaccaacgcc agcacgatct gcttgcaatc
     1681 gctgcggtgg tcccgggaat agccgcggcg ggccaggtcg ttctcctcgg ccagtccctc
     1741 gaagtaggta ctggtgaggt cgtagagcag caggtcgtag tcgagcccga acagggttcc
//
LOCUS       DATAQL010000940         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_555686, whole genome shotgun sequence.
ACCESSION   DATAQL010000940 DATAQL010000000
VERSION     DATAQL010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555686"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VHS97_16350"
     CDS             complement(<1..237)
                     /locus_tag="VHS97_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449827.1"
                     /translation="MATVTQTKLLTAEEFMAADLGEGTFELVRVEIVEVPPPMPEHGV
                     VCANVAGSLWDYGRKSGYGYALSIDSAVVTERGPD"
     gene            complement(292..885)
                     /locus_tag="VHS97_16355"
     CDS             complement(292..885)
                     /locus_tag="VHS97_16355"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3449828.1"
                     /translation="MATVTQTKLLTAEEFMAADLGEGTFELVRGEIVQVPPPKPEHAR
                     VCMKVGWVFENYGVASGYGYCLFESAVSTERGPDTVRGPDICFYSHARWPESEVGTGL
                     PPVPPDLAVEVVSPTNRPGEMQRKVTEYLEAGVLLVLVVDPARRKAALYRAGEVFPTI
                     LSENEVQENLPELPGFVCSLADFFVPVSKNESGPVAL"
     gene            complement(940..2733)
                     /locus_tag="VHS97_16360"
     CDS             complement(940..2733)
                     /locus_tag="VHS97_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEX3449829.1"
                     /translation="MINQKLKDVVVSRILPSIRTPGQYVGGELNSIVKDHRTGRGSVC
                     LAFPDTYALGMSHHGLQVLYSLMNAADWACERAFMPLPDFEEALKQHGLPLYGLETFT
                     PLCQFDVLGFSLQYEICYTNVLLMIELGGIALHAEDRDPDDTLVIAGGPGAQNPELLA
                     PFIDLFVIGDGEPSLPVVCDLWKSMQGSGLSRGDKLARIAATVDWAYVPRFYDPIYRE
                     DGTILEIRRSRDDVPEEIKPCVIRDLESIPLPTRPIVPFVETTHDRIAIEIMRGCPWL
                     CRFCQSTVIKRPLRHRTVETIVQAALESYHATGYDEISLLSLSTSDYPDFEELVTRMS
                     EVFAPLGVKISLPSLRITEMLKKIPALLAEGHRSGLTLAPEVARDDMREQIRKPINNQ
                     DLYDGCAEAFRRGWRKVKLYFMCGLPGERTADLDGIVAMAETIARIGKDVTGRYAEVT
                     ASVSNFIPKPHTAYQWNGMQSREYFIWAHKYLRSKVKIRAVTVKAHDIERSMLEGILT
                     RGDRRVASALEAAYRRGARLDAWSEHFKPKLWWEIFAEHDIDVPFYSHRDRPIDEVLP
                     WDHIRIKFGRDYLVKEQNRSVVQLEEMAGAV"
BASE COUNT          612 a          947 c          975 g          606 t
ORIGIN      
        1 gtccgggccg cgttcagtga ccacggctga gtcgattgac agtgcatatc cgtaaccgga
       61 cttgcgtcca tagtcccaca gacttcccgc gacattggcg caaaccacgc catgttccgg
      121 cattggtggc ggcacttcaa caatctccac tcgaacgagc tcaaacgttc cctcgccgag
      181 atcggccgcc atgaattcct cggcggttag tagcttggtc tgggttacgg tcgccatgct
      241 tttgctccgg gtgagctcgg ttcgctgttg ctgagccacc acacgatcgg gttagagtgc
      301 gacaggccca ctctcgtttt tggaaactgg aacaaagaaa tcggccaggg aacagacgaa
      361 accgggtagc tccggaagat tctcctggac ttcattttcg ctcaagattg taggaaacac
      421 ctctccagcc cggtatagcg cggcttttcg tcgggcggga tcgacgacca ataccaagag
      481 aacaccggct tctagatatt cagtgacctt cctttgcatt tcgccggggc gattggtcgg
      541 cgagacgacc tcgacggcca gatcgggcgg gacaggcggc aggccggtcc cgacttcgga
      601 ttcgggccag cgggcatgac tgtagaagca gatgtcgggg ccgcgcacgg tgtccggacc
      661 gcgctccgtt gaaacggccg actcgaacaa gcagtagcca tatccggacg ccacgccgta
      721 gttctcgaaa acccatccga ccttcataca aacacgtgcg tgctcgggct tcggtggcgg
      781 cacttggaca atctcccctc gaacgagctc aaacgtcccc tcgccgagat cggccgccat
      841 aaattcctcg gcggttagta gcttggtctg ggttacggtc gccatgactc atctcccacg
      901 ggtcgcccac ctggacacct tgagatcatc atccaccaat caaaccgcac cagccatttc
      961 ttcaagctgt acaacggagc gattttgctc cttaaccagg tagtcgcggc cgaacttgat
     1021 gcggatgtgg tcccagggaa ggacctcatc gatcggccgg tcccgatggc tgtagaatgg
     1081 cacgtcgatg tcgtgctcgg caaaaatctc ccaccagagc ttgggcttga aatgttcgct
     1141 ccaagcgtcg agtcgggctc cacggcgata ggcggcctcg agcgcggacg cgacccggcg
     1201 gtcgccccgg gtcaagatcc cttcaagcat gctccgctcg atgtcgtggg ccttgaccgt
     1261 caccgcgcgg atcttgacct tggaccgcag atatttgtgg gcccagatga agtattcgcg
     1321 cgactgcatc ccgttccact ggtaagccgt atgcggcttg ggaatgaagt tggacacgct
     1381 cgccgtgact tccgcgtaac ggccggtgac atccttgccg atcctggcga tcgtttcggc
     1441 catcgcgacg atgccatcga ggtcggccgt gcgctcgccg ggcagcccgc acatgaagta
     1501 aagcttcact ttgcgccagc cgcgccgaaa cgcctcggcg cagccgtcgt aaaggtcctg
     1561 gttgttgatc ggcttgcgga tctgttcccg catgtcatcg cgcgcgacct caggggcgag
     1621 agtcaacccg ctgcgatgtc cttcggccag aagcgcgggg atctttttga gcatctcggt
     1681 gatccgcagg ctcgggagtg agatcttcac ccccagcggg gcgaaaacct cgctcatccg
     1741 cgtcaccagc tcctcaaagt cggggtagtc gctcgtggaa agcgaaagca ggctgatctc
     1801 gtcgtagccg gtggcgtggt agctttcgag cgcggcctgg acgatcgtct cgacagtgcg
     1861 atgacggagc gggcgcttga tgacagtgct ctggcaaaac cggcacaacc aagggcaacc
     1921 gcgcatgatc tcgatggcga tccggtcgtg ggtcgtctcc acaaatggaa cgatcggtcg
     1981 ggtcggcaac ggaatgctct cgaggtcgcg gatcacgcag ggcttgatct cctccggaac
     2041 atcgtcacgg ctgcggcgga tctcgagaat cgtaccgtct tcgcggtaga tgggatcgta
     2101 aaaccgcggg acgtacgccc agtcaacggt tgcggcaatc cgggccagct tgtcaccgcg
     2161 cgaaaggccg gatccttgca tcgatttcca caggtcgcag accaccggca ggctgggctc
     2221 gccgtcgccg attacgaaca gatcgataaa aggtgcgagc aattctggat tctgggcacc
     2281 gggcccgccg gcgatgacca gcgtgtcgtc gggatcgcga tcctcggcgt ggagggcgat
     2341 cccaccgagt tcgatcatca acaagacatt cgtgtagcag atctcgtact gaagcgaaaa
     2401 gcccaggacg tcgaactggc aaagcggagt aaaggtctcc aagccgtaga gaggcaagcc
     2461 gtgctgcttg agtgcctctt caaagtcagg cagcggcatg aacgcccgct cgcaggccca
     2521 atcggccgcg ttcatcaggc tgtacagtac ctgaagcccg tgatggctca tccccaaggc
     2581 ataggtgtcg gggaacgcca ggcacacgct gccgcgcccg gtccggtggt ccttgacgat
     2641 gctgttgagc tcgccgccga cgtactggcc gggggtacgt atactgggca agatccgcga
     2701 aacaaccacg tccttcagtt tttgatttat catgagagag tgactccggc gacaaccccg
     2761 ctagggcccg gcgtcgagcc gttcggcact tgtccggcct ggattcgatg gatggcacct
     2821 tgacggagat cggaacgatc gggcgtgcgg aagctaaaga tgtatgttaa cccgtcggcc
     2881 gttttctgga tagttgcccg gaagagctaa cgggtggccg gcgggccaac ggattctggc
     2941 aatcgagttg gttgccccaa aacgctctga gccactgcga tgcgctgtcc gcgccgttgg
     3001 agaccttcgg tcgcgccgga gggcggggtc gggagacccg cgcccagcat ccgcgcggcg
     3061 gagggcgagg taggtcgggc tttctagccc gacgactgcc agccaagatc cgcgcgcggc
     3121 ggagggcgag gtaggtcggg
//
LOCUS       DATAQL010000941         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2234041, whole genome shotgun sequence.
ACCESSION   DATAQL010000941 DATAQL010000000
VERSION     DATAQL010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234041"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VHS97_16365"
     CDS             <1..578
                     /locus_tag="VHS97_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3449830.1"
                     /translation="ARSPDGWQAVEFRFGTERAPVDPASKAYVEVSFTEGRPVLEALL
                     ELNERIYTEFRFRSGVTTLSTPVSQVMARREGVCQDFSHAMISGLRGIGIPARYTSGY
                     IRTKPPPGQTKRHGADQSHAWVGCWLGPEHGWVDVDPTNGIVVKDEHVLLGWGRDFSD
                     VSPVRGVILGGGDHTVRVGVDLEPVDDEVVE"
     gene            575..1258
                     /locus_tag="VHS97_16370"
     CDS             575..1258
                     /locus_tag="VHS97_16370"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3449831.1"
                     /translation="MTEIRLVSTLAVMGAMPELIARYKAAGSPINADFAPTLALLDRL
                     RAGEAADVAILTAQGIDDLIAEGVIASGTRTDIARSFVGLAVKAGAPKPDITTVERFK
                     ATLLEARSVAYSKIGASGIFFAGLLDRLGIAGAVRSLIVPSGFTAERLITGEADLAVQ
                     QISELMMVPGIEVVGPLPPEIQTVAIFAGGLLTRSSHPEYATALLRSFASPDIAPVLQ
                     RTGLEPARS"
     gene            1340..>1701
                     /locus_tag="VHS97_16375"
     CDS             1340..>1701
                     /locus_tag="VHS97_16375"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEX3449832.1"
                     /translation="MPVLTVTHTTTYRYANPVRFGEHRMMMRPRDSHDLRLLHTALII
                     RPAGKIRWIHDVFGNSVAIARFNEPAAELRVESKFRAEHYPLAESGVEIEAYARQHPF
                     SYDASEIPDIGRAAERHYA"
BASE COUNT          301 a          607 c          501 g          292 t
ORIGIN      
        1 cagcccgcag cccagacggc tggcaagcgg tggaattccg gttcggaacc gaaagggccc
       61 cggtggaccc tgcctcaaaa gcctatgtcg aagtttcctt caccgagggg cgcccggtgc
      121 tggaggctct gctggagctc aacgagcgta tatacaccga atttcggttt cgctccggcg
      181 tcaccaccct ctccactccg gtctctcagg tcatggcccg ccgcgaggga gtctgccagg
      241 atttctccca tgccatgatc agcggcctcc gaggcatcgg tattccggcc cgctacacct
      301 ccggctacat ccgcaccaag ccgccgcctg gccagaccaa acgccacggc gccgatcaat
      361 cccacgcctg ggtgggctgt tggctcggtc cagagcacgg ttgggtagac gtcgatccaa
      421 ccaacggcat cgtggtcaag gacgagcatg tcctgctcgg ctggggccgc gacttcagcg
      481 acgtcagtcc cgtgcgcggc gtgatcctgg gcggcggcga tcacaccgtc cgggttgggg
      541 tcgatcttga acccgtggat gacgaggtcg tggaatgacc gagattcgcc tcgtctcgac
      601 gctcgcggtc atgggcgcca tgccagaact aatcgcccgt tacaaagccg cgggaagccc
      661 aatcaacgcg gacttcgcgc ccactcttgc cctgctcgac cgtctcaggg ccggcgaggc
      721 agccgacgtc gcgatcctca ccgcgcaagg gatcgacgac ctgatcgcgg aaggggtcat
      781 agcgtccggc accaggaccg acatcgcccg atccttcgtt ggtttggcgg tcaaagcggg
      841 cgcccccaag cccgatatca cgacggtcga acgcttcaag gccacgcttc tggaggcccg
      901 ctccgtcgcc tattcgaaga tcggcgccag cggcatcttc ttcgccggtt tgctcgaccg
      961 cctgggtatc gccggcgcgg tcaggagctt gatcgtccca tccgggttta ctgccgaacg
     1021 cctgatcacc ggtgaggccg acctcgccgt ccaacaaatc agcgagctga tgatggtgcc
     1081 cggcatcgag gtcgtcggtc cgctgccacc cgagatccag accgtcgcca tcttcgccgg
     1141 cggtctgcta acccggtcgt cacaccccga atacgcgaca gcgctgctgc gctccttcgc
     1201 ctcgcccgac atcgctccgg tcctgcagcg cactggcctc gaacccgccc gatcgtgaca
     1261 gagatgataa atcgcgcagc ccgggcatcc gaaccggcgc cgaaccgtta ggctgcggcg
     1321 cacccgcaga ggatcctccg tgcccgtcct gaccgtaact cacactacca cctaccgcta
     1381 cgccaatccg gtgcggttcg gggaacaccg gatgatgatg cgcccccgcg atagccacga
     1441 tctgagactg ttgcacaccg cgctgatcat ccgtccagcc ggcaagatcc gctggatcca
     1501 cgacgtcttc gggaactcgg tggccatcgc tcgcttcaac gagcccgcgg ccgagctccg
     1561 cgtggagtcg aaattccggg ccgaacatta tccgctcgct gaaagcggcg tcgaaatcga
     1621 ggcgtacgcc cgccagcatc ccttcagcta cgatgcctcc gagatccccg atattggccg
     1681 cgccgccgaa cgccactacg c
//
LOCUS       DATAQL010000942         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1777323, whole genome shotgun sequence.
ACCESSION   DATAQL010000942 DATAQL010000000
VERSION     DATAQL010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777323"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..823)
                     /locus_tag="VHS97_16380"
     CDS             complement(416..823)
                     /locus_tag="VHS97_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449833.1"
                     /translation="MAIINTFQKCLDSPYLFEDKEAPNRIVDLLAKAGDRLDAAVNIQ
                     TTGQGDPADVVFLSYEAMFSCVRALVYAKGYREFGLRCLLLACEGLYVRTGELDSVHL
                     RRFETAQRLKMSPAEAVEASSAFVKRTLELLGQ"
     gene            complement(930..2753)
                     /locus_tag="VHS97_16385"
     CDS             complement(930..2753)
                     /locus_tag="VHS97_16385"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX3449834.1"
                     /translation="MPTNPPSGLDFRDIFFLAPEIVLTLWGFVVLVVDRVLARKLTAT
                     RRQRAIGGLSLAGVGIALVAAAIVCFVPLSARAYPPDENSWLSPQTLSYLADSDPVIF
                     FGTLAGDVQTAFFNVLYVLLLGLILGMSMSWSFTDEWGEYFALLFWATVGMMLLTASE
                     ELVTLFLTLETMTICLYLSTALEKTKRRSAEGGLKYFVYGSVSSALFLFGLSLIYGMT
                     GTTSFDGIRSIIAATNSSGLADNVAGATAILLLLVGFGFKVAAVPFHQWAPDAYEGAP
                     APVTAWIATGSKLASFVALMKVFLHALLPWSSPTTSIMGPGWVGIIAIVSAVTMTYGN
                     FAALAQTNLKRMLAYSSIAHAGYILVGVAAVSVSMKGPAAAAAVLYYLIVYAFANVGA
                     FAVAAWLARDLDSDNIDDLNGLGFQQPLLAVCILILMLSLIGIPPLAGFFGKLYVFME
                     ALNQTHESQRHTLIGLVALGLLNSVVSAFYYVRVLKAMFLREAGTKRMAPPDRGIALP
                     IVLGAIVVAVFGLMPESLIGVMQAASVPMLTSPARILRSERMSAMEKPSPNPARPPAV
                     RFEYTEEQKRMMADPATAKGGSSPKSAPAKKQATSAKTKTD"
     gene            complement(2784..3113)
                     /gene="nuoK"
                     /locus_tag="VHS97_16390"
     CDS             complement(2784..3113)
                     /gene="nuoK"
                     /locus_tag="VHS97_16390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX3449835.1"
                     /translation="MGNDIPLSWFLYFAAASFAIGLAGVLVRRNAIAVLMAIEIMLNA
                     ANINLVAFWRYGPERPEAGPLAGVILAIMVLTVAAAEVAVGIGLILLCYRRWHSADVD
                     RYDSMAG"
BASE COUNT          734 a         1003 c          993 g          600 t
ORIGIN      
        1 tgggggatgc atttcccccc cttgtgaagg ggctcacagg gaggggcttt tctgaaaact
       61 gccagggagg ctataggccc cgatacagca cactcgcggc cgatgcacgt tactggctcc
      121 ctctctccaa ggagcgggag ctagtggtga cgagtaccca tccctccaag ttgtagaggg
      181 gggcagcgag ctgtcagcca cagtgttttc agaggggatc caagcggaaa tacttgccgg
      241 ttaatcatca aagcaaattg gcgatgtacg tttgttcgat gaccacagca gacgcttgtt
      301 tattgatcac aacttgagaa gacatgtgcc tgattgctca ctgaccaccc ccctggcccc
      361 cccttcacaa gggggggaaa aagcagccac gaaggcgcac attatgattc ggccgctatt
      421 gacccaataa ctcgagcgtc cgcttaacaa acgcagatga ggcctcgacg gcctccgcgg
      481 gcgacatctt gagacgctgg gccgtctcga acctgcgtaa atgcaccgag tcgagttccc
      541 cggtgcgaac gtaaagtccc tcgcacgcca agagcaggca gcgcaggccg aattcacggt
      601 agcccttggc gtaaaccaag gctcggacgc aactaaacat ggcctcgtag ctcaagaaaa
      661 cgacgtcggc cgggtccccc tgtccggtgg tttggatgtt aaccgcggca tcgagccggt
      721 cacccgcttt ggcaagcagg tcgacgatcc ggttgggcgc ctccttgtcc tcgaagagat
      781 aggggctatc aaggcacttc tgaaaggtgt tgatgatggc catggccgtt ggcgtctcgt
      841 ctcgtctcgg ttctgggaca gtcttcattt ccctgtgatc gaccgacaga ccgcggcgcg
      901 acgcccggtt tgtcggatgt ctgggctagt cagtcagtct tggttttggc tgacgtcgct
      961 tgtttcttgg ccggggcgga cttgggacta ctgccccctt tagccgtcgc ggggtcggcc
     1021 atcatccttt tctgttcctc ggtgtactcg aaacgtacgg ccggcggtct tgctgggtta
     1081 ggtgaaggtt tctccatcgc cgacatgcgc tccgaccgca gtattctagc cggactggtc
     1141 agcatcggca cggaggccgc ctgcatcacg ccgatcagcg actccggcat caagccgaag
     1201 accgccacga cgatggcccc cagcacaatg ggcagggcga tccctcggtc gggcggggcc
     1261 atccgcttcg tgccggcctc gcggagaaac atggctttca agacgcgcac ataatagaaa
     1321 gccgatacca ccgaattgag gagcccaagc gccaccagcc cgatcaaagt gtggcgctgt
     1381 gactcgtggg tctggttgag cgcttccatg aagacgtaga gcttgccgaa gaaacccgcc
     1441 aagggtggaa tgccgatcaa cgacagcatg aggattaaaa tgcaaacggc caacaggggc
     1501 tgctgaaaac cgaggccgtt caagtcatcg atgttgtcgc tatccaggtc acgggcgagc
     1561 caggcggcaa cggcgaatgc cccgacgttc gcgaacgcat acacaatgag atagtaaagt
     1621 accgcggctg ccgcggccgg tcccttcatc gaaacgctga cggccgccac tccgactaag
     1681 atgtaaccgg catgggcaat cgacgaataa gccagcatcc gtttcaaatt tgtctgagcg
     1741 agcgccgcga agttaccgta ggtcatcgtg acggccgaaa cgatcgcgat gatgccgacc
     1801 cagcccggcc ccatgatgct cgtcgtcggg ctcgaccacg gcaaaagtgc gtgcagaaag
     1861 accttcatga gcgcgacaaa gctggctagt ttcgacccgg tcgcgatcca ggccgtcacc
     1921 ggtgcaggcg cgccttcata ggcatcggga gcccactggt gaaacggcac tgcggcgacc
     1981 ttgaagccga atcccaccaa gagcagcaag atcgccgtgg cgccggcgac gttgtccgcc
     2041 agaccggagg aatttgtcgc ggcgatgatc gacctgatcc catcgaacga agtcgtgccc
     2101 gtcatgccgt aaatcaggct caggccaaac agaaaaagcg ctgacgagac cgatccgtag
     2161 acgaagtact tgagccctcc ctccgccgac cggcgtttcg ttttttcaag cgccgtgctc
     2221 agatagaggc agatcgtcat cgtctcgagc gttaggaaca gggtgacgag ctcttccgag
     2281 gctgtgagga gcatcatgcc gaccgtggcc caaaacagga gtgcgaaata ctcgccccac
     2341 tcgtccgtga acgaccagct catcgacatg cccaggatca aacccagtaa caagacgtag
     2401 agaacgttga agaaagccgt ctgtacgtct ccggccagcg tgccgaagaa gatcacggga
     2461 tcggagtcgg ccaggtagga caaggtctgg gggctgagcc aggagttttc gtccggggga
     2521 taagctcggg ccgaaagcgg cacaaaacaa acgatcgcgg ccgccacaag cgcgatgccc
     2581 acgcctgcca gcgacaggcc gccgatcgcc cgctggcggc gagtcgcggt cagctttcgc
     2641 gcaagtaccc ggtccacgac caggacgacg aagccccaca gcgtcaagac gatctcgggg
     2701 gccaggaaga agatgtcgcg gaagtccagg cctgagggcg gatttgtcgg catcgcgtcg
     2761 tcactttcaa gaggttctgg gggtcatcct gccatgctgt cgtagcgatc cacatccgct
     2821 gaatgccacc gccggtagca aagcaaaatc aaaccgatgc cgacggcgac ctcggccgcg
     2881 gcgacagtga gaaccatgat ggccaggatt acgcccgcca gcggtccagc ttcgggccgt
     2941 tccggcccat aacgccaaaa cgcaacgagg ttgatattcg cggcattgag catgatctcg
     3001 atcgccatca acaccgcaat tgcattgcgt cgaacgagaa caccggccag gccgatcgcg
     3061 aatgaggcag cggcgaaata gaggaaccag gatagtggta tgtcgttgcc catgcatcag
     3121 ctcttttcgg ttcaaaccct gactctcaaa tctcgaccag ccaagaagcc ggaatctacg
     3181 cggatcgtga caggacgtcg atcatttaat tcacacccgg agtcgtcgac gacaaggccg
     3241 caactgcgcg ctccggcgcc ggaggatcgc cctccgccgc ggaggccagt tcgcccgcgc
     3301 ttcccttggt tccgcgcggc cgtgccggtt
//
LOCUS       DATAQL010000943         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2538138, whole genome shotgun sequence.
ACCESSION   DATAQL010000943 DATAQL010000000
VERSION     DATAQL010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538138"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VHS97_16395"
     CDS             complement(<1..497)
                     /locus_tag="VHS97_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449836.1"
                     /translation="MSTSAENGGNKPQSWSGRIIGAFTGPAPSGNSGEDGEKPDILPP
                     EERKAAMSSLDPQEVKWSLAALALATLTGIGFPAYYIVADPLTKVDGKYVAVSPDAAL
                     IGAVLIVLCAIGFVALWKRKRTLLAFDLFLTGFALTQFIQLFGVAYIFLGGFLLLRAW
                     RINKYG"
     gene            601..674
                     /locus_tag="VHS97_16400"
     tRNA            601..674
                     /locus_tag="VHS97_16400"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:635..637,aa:Val,seq:cac)
     gene            698..1978
                     /locus_tag="VHS97_16405"
     CDS             698..1978
                     /locus_tag="VHS97_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449837.1"
                     /translation="MRVVEHFVDVLRRARYVREGIGFFRARTRTIGRFVRRATPLRQG
                     IERIVEHFFGPRKQVPVTVEREAHGRVSRSHRNLLRMRAGSDPECDRRVAEVVRPEVA
                     QTGSLRRRHPEATTPDVERHRAALRRREDEIFGRAGERGQMIAQWLRGDPGERDGAIS
                     RSSLGRTEVQVTADVDHCFGDRHLAPKDVDPLGSEAEELACPQTPVSGEKHDGLVARV
                     HGGGERVNLGGGEEAHLDAFDPGHPDALARRRWHEAGLDGRSHDLRHQLTCFCHGRRG
                     QAGGGELRDPGAHGHVPDVAQRHRPEVGEEVTLQVPQVSYAARGPQVDRRGAPLLGPV
                     REQHRGAQGVDPEPSADFGLDTREVTVGVGFECERHGSDVSPPEMPVAGLPTTRRELP
                     QGAELSSIAHRNLLSTKRSLCPSDVTRISTLRTV"
     gene            complement(2455..>3259)
                     /locus_tag="VHS97_16410"
     CDS             complement(2455..>3259)
                     /locus_tag="VHS97_16410"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3449838.1"
                     /translation="GEEVLDVPPVLASRVRVLATTRSNKNDPNDALSVALAALRTPHL
                     RSVEPAEHSEVLRLLSKRHSDIGSQRIRLVCRLHALFQELVPGGMAKKMNASDAQALL
                     ARLKPATPTQQVRYDLATELLDELWRADAQLKASERRIKAAVKVSETSVTQIYGVGPV
                     LAAQLIGYSGDVRRFTNRDSYASYNGTAPVEFSSGGRVVHRVSQRGNRMLNHAVHMAA
                     VTQLRQPTTQGRIYFERKLSEGKTKREALRSLKRHISNAIYRQLLLDAQ"
BASE COUNT          585 a         1012 c         1089 g          573 t
ORIGIN      
        1 ccgtacttgt tgatgcgcca ggcccgcagc aggaggaacc cacccaagaa gatgtaagcg
       61 accccgaaca gttggatgaa ctgcgtcagc gcgaagccgg tgaggaagag gtcgaaggcc
      121 aacagcgtcc gcttgcgctt ccaaagtgcc acgaaaccga tggcgcacag cacgatgaga
      181 acggcgccga tcagggccgc gtcgggggag accgcgacgt acttgccgtc gactttcgtg
      241 agcgggtcgg cgacgatgta gtaggccggg aatccgatgc cggtgagcgt cgccagggcc
      301 agcgcggcca agctccattt gacttcctgg gggtccaggg aagacatggc ggccttgcgc
      361 tcttcggggg gcagtatgtc cggcttttcg ccatcctctc cggagttccc cgaaggggcg
      421 ggacctgtga aggccccgat gatgcgaccg ctccagctct gcggcttgtt gccgccattt
      481 tcggcgctgg ttgacatggc tacctcagag taccggtgtg atcagaggcc cccaaatcac
      541 cctggaccgg aaggtcttgg ggctcccgca ggctacggtg gtgctccggc aaatccgcca
      601 gggcgcttag ctcagctggt tagagcgcag ccttcacacg gctgaggtcg tgggttcgag
      661 tcccgcagcg cccactcaat aagtcctgtt cacggccttg cgagtcgtcg agcacttcgt
      721 cgacgtcctc cggcgtgcgc gctacgtgcg cgaaggaatc ggcttctttc gcgcacgaac
      781 gcgcacgatc ggccgattcg tccgacgcgc gacccctttg cgccagggca tcgagcgaat
      841 cgttgagcac ttcttcggac cccggaaaca ggtgcccgta acggtcgagc gtgaagctca
      901 cggacgtgtg tcccgctcgc accgcaacct ccttcggatg cgcgccggca gcgatccaga
      961 gtgcgaccgc cgtgtggcgg aggtcgtgcg gccggaggtg gcccagaccg gcagccttcg
     1021 tcgccggcac ccagaagcga cgacgccaga cgttgagcga cacagggccg ccctccggcg
     1081 ccgtgaagac gagatcttcg ggcgtgcggg cgaacgtggc cagatgatcg cccagtggct
     1141 cagaggcgac ccgggggagc gggacggtgc gatttcccgc tcgagtcttg ggcggaccga
     1201 agtgcaggtg accgccgacg tcgaccactg tttcggcgat cgccacctgg cgccgaagga
     1261 tgtcgacccg cttggctctg aggccgaaga gctcgcctgc ccgcagaccc ccgtaagcgg
     1321 cgagaagcac gacggcctgg tagcgagggt ccatggcggc ggcgagagag tcaacctcgg
     1381 tggtggtgag gaagcgcatc tcgatgcgtt cgatccgggg catcctgacg ccctcgcacg
     1441 gcgacgttgg cacgaggccg gcctggacgg cagatcgcat gatcttcgcc atcagctgac
     1501 ctgcttttgt cacggtcgac ggggccaagc cggtggtggt gagctcagag acccaggtgc
     1561 gcacggtcat gtacctgatg tcgcccagcg ccatcgtccc gaagtggggg aggaggtaac
     1621 gctgcaagta ccccaggtct cttacgcggc tcgagggcct caggtcgacc gtcgtggcgc
     1681 gccactcctc ggcccagtcc gagaacagca ccgtggcgcc cagggggtcg atccagagcc
     1741 ctcggcggac ttcggtctcg acactcgaga ggtaaccgtt ggcgtcggct ttgaatgcga
     1801 acgtcacggg agcgatgtgt cgcctcccga gatgccagta gctggcctgc caacgacccg
     1861 aagggagctt cctcaaggag ccgaactgtc gtcgattgct catcgcaacc tcctgtctac
     1921 gaagcgatca ctatgcccgt cggatgtcac acgaatcagc actctgagaa cggtctgact
     1981 gagacgagaa ttgcgagtga ccgtgaccag taagcagagc atcgcaacga atagcaatga
     2041 cgaacagaga gcaacaacga gcaacaagtg gtccccaaag aaatccctga tcaggcccca
     2101 gatgtccgcg ttcttctgta ctgctttaaa ccgcctcgct cgagccggct gctgcggcgc
     2161 cagctcggca ggcggatggg gctctggaat agccgaccga ctgagttcgg gctcgagttt
     2221 caaccccaga acttcctgcg ggtccggcta tcagaccgcg gcagaccgaa tcctctttgt
     2281 gtcaagaccc ctgtttcggt gctgttgggg cgcgccgaat cgagccgtca cgaacgaggc
     2341 gacaggcgta ggatttgact gggttcggga gtgacttcgc cgaagagccg gctgcgcagg
     2401 gtgagaggcc ggtcacgcag gctttagtcg tccctgagtg tcctcccgga ccccctattg
     2461 agcgtcgaga agcagctgcc ggtagatggc gttggagatg tgacgcttca acgatcgcag
     2521 cgcctctcgc ttggtcttgc cttcacttag tttgcgctcg aagtagatcc ggccctgggt
     2581 cgtgggctga cggagctggg tgacggccgc catgtggacg gcgtggttga gcatccggtt
     2641 gcctcgttga gaaacgcggt gcaccacccg cccgcccgag gagaactcga cgggtgccgt
     2701 gccgttgtag gaggcatagg agtcccggtt ggtgaagcgc cggacgtcgc cgctgtagcc
     2761 gatcagctgc gcggcgagaa ccggcccgac cccatagatc tgcgtcaccg aggtctccga
     2821 caccttgacg gccgccttga tacgcctttc agaagccttc agctgcgcgt cggcgcgcca
     2881 gagttcgtcg agcagctcgg tggccaagtc gtagcgcacc tgctgggtcg gggtcgccgg
     2941 cttgagcctc gccaggaggg cttgtgcgtc agaggcgttc attttcttcg ccattccccc
     3001 cggaacgagc tcttggaaga gagcatggag ccggcacacc agtcggatgc gctgactccc
     3061 gatgtcgcta tggcgcttgg acaagaggcg cagcacctca ctgtgctcgg ctggctcgac
     3121 cgatcgcagg tgtggggtgc ggagcgcggc cagagcaacc gacagcgcgt cgttgggatc
     3181 gttcttgttc gacctcgtgg tggccaacac gcggacccga gaggccaaca cgggcggcac
     3241 gtcgaggact tcttcgccc
//
LOCUS       DATAQL010000944         7023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3609646, whole genome shotgun sequence.
ACCESSION   DATAQL010000944 DATAQL010000000
VERSION     DATAQL010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609646"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VHS97_16415"
     CDS             complement(<1..1173)
                     /locus_tag="VHS97_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3449839.1"
                     /translation="MCVVWFLTDPGQMKRLAAVAPGRASPRTGEVTSRSLTAPAPQAS
                     IPLYSEDWTEDSGQGLALRSSRPITDPQSLEQVRTSDEGRGQRGIDRVRQELVAVDRR
                     TPAGRARVIQLEIFLGLLHMSVGEFTEADRRFAEAQAADPDCPDLLRANIEALRGVAA
                     LRRGETENCVACCIGASCIYPLAPEAIHQRPSGSREAIGRFTTYLKQRPADLGVQWLL
                     NVAYMTLGDYPHRVPSQYLIPLGPFQTDGGVRRLENIAGRVGIGARGANMSGGCIVDD
                     FTGDGLLDVFYSTKDPTQGCALFVNRRDGTFEDRSTRAGLEPQVGALNCNHADYDNDG
                     DLDVLLLRGGWENPRRPSLLRNEGEGRFTDITIAACLCEPIASQAAAWADFDCDGHV"
     gene            2302..3495
                     /locus_tag="VHS97_16420"
     CDS             2302..3495
                     /locus_tag="VHS97_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449840.1"
                     /translation="MHLKRRRGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRSQC
                     TNNLKQIALALHNYVSTNQTLPTMYIPYSPVGGMNTGQTYSPLARTLPFMEQNAIYNS
                     INFNVAPRWGASGSDIVSSMNGSTADCDQFGLMNASAAGNQITSFLCPSDTDLSNLTG
                     FIFYPGGPMQLVGRFNYPANGGTNVFRAGASGVSNGAVYVPTASRGFAATGMPGCCEM
                     APGNGGASLVGGSYETPRDLAQLTDGTSNTAAYSESIRGNGSNTPATCPVTLGLIYTS
                     PSPANAFAGLLNNDFQQAAACDRAVQGTNSYTWKGDWWLADLFPYSHTTTPNRKSCFY
                     NDVGGRPWSAITNVISASSRHPGGANVAFCDGSVRFIKSTVDARTWAAIGTINGGEVV
                     SSDAY"
     gene            3627..5477
                     /locus_tag="VHS97_16425"
     CDS             3627..5477
                     /locus_tag="VHS97_16425"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX3449841.1"
                     /translation="MTSVVRRTPFRPLTAAFFLGLPVILAAGCGYEPAISARDVAAAR
                     ATKVAGAAITPEPSRAAAKRPAPGSAAEDGHSTIRFREVERSAGITFTHVSGNSPDKH
                     YPTANGSGVAMFDYDGDGRLDLYFATTRNLPLHAPTTSQGNKLYRNQGDGSFEDVTDR
                     AGVGFRGFCHGVAVGDVDNNGYPDLYLTNYGPNVLYLNNGDGTFRTARDFGAECRSWS
                     TAAAFLDYDGDGWLDLYVSSYGHWVFDEPHPFCGNEAQGVRIYCSPITIPPAPHFLFR
                     NRGDGTFEDVTQRAGVLRRDGRGMGVVAADVNRDGRVDLYVANDMCPHFLFLNRGDGT
                     FEDVTDTSGAALTESGHVQGGMGVDVEDLDGDGYPELFVTNFDGEYNTIHRTTDGRIF
                     EDVSARSGIVPDSLADVGWGCALLDLDNDGWPDMITVNGHIYDNLAQVGRDGEYAQRP
                     KIWRNRGRGRFKLVADAGPFFDVGHAARGAAFGDLDNDGDIDVVINRMDSRPAVLLNE
                     SQPAHWIRLELVGSRSNRSAIGASVAVHAGDRVLHRQVKGGGSYASANDPRLLVGLGG
                     ADRVDRVEIRWPSGRSTTLTNLALARTHRVVEPDGLVGSDAPSATDGRRR"
     gene            5477..6778
                     /locus_tag="VHS97_16430"
     CDS             5477..6778
                     /locus_tag="VHS97_16430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449842.1"
                     /translation="MSSTIDSGRKRIIRYVLTGLTLALGVGGLAWGVRQSLRSRSSLD
                     EAIGLAEAGRLVEAEAKVRARLATDPDAAAAHLLLAQIILKRPDQPSPPSDRCASPSG
                     REALDHLDRVHAQNPAMDVIFHLCRGDALKRLMRFDDAEADWLEALRIDTTAPEVGFN
                     LLDLYYFEGREEESRRLALRLYRVEPDPHDRATLLVELLRLDVRPPAPGSIEKLLEPV
                     VRHNPGDLHTGVAMGLALTRANKVDMGIDQLRRVVRTHPDSVEAWDGLLTGLDESGQV
                     DVIEEELERLPAGLSDAPRLLKHKARIAQDGNHWSEAVGLFRRARANEPSNRVVEYRL
                     SRALRHAGETDEANRLEQRVRRREIALQELRPLFDKATATPDLGTRPYPELYHQIAEV
                     RERMQLPEEAMAWHRLVLGNDPKNEVSLAALARLGNESDSL"
BASE COUNT         1296 a         2205 c         2189 g         1333 t
ORIGIN      
        1 gacatgcccg tcgcagtcga agtcggccca agcggcggcc tgggaggcaa tcggctcgca
       61 caagcacgcg gcgatcgtga tgtcggtgaa ccgaccttct ccctcattac gtagcaatga
      121 gggccgtcgg gggttttccc acccgccccg caagagcagc acgtccaagt caccatcgtt
      181 gtcgtagtcg gcgtggttgc aattcaaggc cccgacttgc ggctcgagac cggcgcgggt
      241 ggaacggtcc tcgaacgtgc cgtcccggcg gttgacgaac agggcgcagc cctgcgtcgg
      301 gtctttggtc gagtaaaaga cgtcgagtag cccatcgccg gtgaagtcgt cgacgatgca
      361 gccccccgac atgttcgcgc cccgcgcgcc aatgccgact cgcccggcaa tgttctccag
      421 ccgacgtacg ccgccgtcgg tctggaacgg ccccagcggg atcaagtact ggctcggcac
      481 gcggtgagga tagtcgccca gggtcatgta ggcaacgttc aagagccact ggacgcccag
      541 gtccgcgggt cgctgcttga ggtaggtggt gaagcggccg atcgcctcgc gcgatccgga
      601 ggggcgctga tgaatcgcct ccggcgcgag cgggtagatg cagctcgcgc cgatgcagca
      661 ggcaacgcag ttttccgtct cgccgcggcg cagagccgcg accccacgga gggcctcgat
      721 gttcgctcgc agaaggtcgg ggcagtctgg atcggccgcc tgcgcctcgg cgaaccggcg
      781 gtcagcctcg gtgaactcgc cgaccgacat gtgcaggagc cccaggaaga tttcgagctg
      841 gatcacccgc gcccgaccgg ccggagttcg gcgatcgacg gcaaccaact cctgtcgcac
      901 tcgatcgatc cccctctggc cccgcccctc gtccgacgtc cggacctgct cgagcgactg
      961 tggatccgtg atcggccgcg aggaccgaag ggccaaacct tggccgctgt cttcggtcca
     1021 gtcctcggaa taaagcggga tgcttgcctg tggcgccggg gctgtcagcg atctcgacgt
     1081 gacctcgccc gttcgcggcg atgcccgtcc cggcgcgacc gcggccagcc gcttcatctg
     1141 gcccgggtcg gtcaggaacc acacgacgca caccgatgct cccacgatcc ctaccataat
     1201 gagcaggaaa cggatcgccc cggtacccgg cttctgcacc gtggtggatg ctgttcgaaa
     1261 agctgacatt gattcgtgct tcggagtcaa tccgacctct gcaggtccct tcgcaacggc
     1321 gtccccgccg tcgcccagaa tcggcgccgc cgtccgcgcc ttcactctac ttccgcagaa
     1381 tcgatgtggc aatccaggct ccgtccttcg attaccactt agcagaccct gcacaacgaa
     1441 ctattaaata cttcgtttta agatcattga cacgtttggc tcacggcaag aaacaaataa
     1501 tgtggatgtc cgcgattttt ttcttgacaa acgtcgtggc tcgatctaac aatcaaactc
     1561 attgcatatt ccgttacatt ttcccgaaat tgtgcgatct catctgcaaa ttggcgttcg
     1621 cctgtagcgg caaaacgcgt aagtcggtta gctgatcgat gttctcacgc tgggttgcgt
     1681 gttcatgctt cgtctgttgc tatgtctttc gggcagcggc tgaccaagcc ggttctcggc
     1741 gggatgggtt agcagcgagg ctcccggcgg atttctggag gccagggcga cgagattgcc
     1801 gggcttctac aatttgtttc ctttggaggt aattgatgaa gagacaattt cgagcgctga
     1861 cgctgccggc gttggcgctg tgttccctgc tgtcgtccgg gtgcggttcg tcgacgacca
     1921 atgaagagaa cttggttagc accagagccc caggtgcaga aaatgctccg gtgtacaagt
     1981 cgtacggcga aaaaatgatt aaagactctg aggaagcggc caagcagcgg gccgcagcca
     2041 agggtgctaa gggaaagcct gctccaacgc caacagcgcc ggctgaggct ccaaagtaga
     2101 catttcgaag tcttaacaat gaaaggatgt tgttggtctg tcattctgaa ctgggaagcc
     2161 ggtcgctcgc tcgtggaaac ggcggtcgac ccgcgctatt cgcgattcgg tcttgcgcaa
     2221 gccggtctcg acgctgtgaa gatcggctgg taatcggtct gagttttctc acttctcttc
     2281 taaggaatcg ggagccgtct tatgcatttg aaacgtcgtc gaggtttcac cctcatcgag
     2341 ctcttggtgg tgatcgcaat tatcgctgtt ctcatcgccc tgctcttgcc cgccgtacag
     2401 gccgcgcgtg aggccgcgcg ccgctctcaa tgcaccaaca atctcaagca gatcgcgctc
     2461 gcgctgcata actacgtcag cacgaaccag acgctgccga cgatgtacat cccgtactcg
     2521 ccggtcggcg gaatgaacac cggacagacg tacagtccgc tggcgcggac gctgccgttc
     2581 atggagcaaa atgcgatcta caactcaatc aacttcaacg tcgcgccgcg gtggggcgcg
     2641 tccggcagtg atatcgtcag tagtatgaac ggctcgactg cggactgcga ccagtttggc
     2701 ctgatgaacg cctccgccgc ggggaaccaa attacgagct tcctgtgccc gtcggatacc
     2761 gatctgtcca acttgacggg cttcatcttc tatccgggcg ggccgatgca gttggtcggc
     2821 cggttcaact atccagcgaa cggtggtacc aacgtattcc gcgccggcgc cagcggggtc
     2881 agtaatggcg cggtctacgt cccaacggcc tcgcggggct ttgccgcaac cggcatgccc
     2941 ggctgttgcg aaatggcgcc cggaaatggc ggcgcatcgc tcgtgggtgg ttcctacgag
     3001 accccgaggg acctcgccca acttacagac gggacgagta acaccgccgc gtatagcgaa
     3061 tctatcaggg gcaatggcag caacactccg gcgacctgcc cggtcacact cggcctgatc
     3121 tacacgagtc cgtcacctgc gaatgccttc gccgggctgc tcaacaacga cttccagcag
     3181 gctgcagctt gtgatcgggc tgttcagggt accaattcgt acacctggaa gggcgactgg
     3241 tggctcgccg acctgttccc ctacagccac accacgacgc ccaaccggaa gtcgtgcttt
     3301 tataatgacg tcggcggacg gccctggtcg gcaatcacga acgtgatctc ggcgtcttcg
     3361 cggcatccgg ggggtgccaa cgtcgcgttc tgcgacggct cggtccggtt catcaagagc
     3421 acggtcgacg cccggacctg ggcggcaatt gggacgatca acgggggtga ggtcgtcagc
     3481 tcggacgcct attgatgtgg gtctgcctga aaacttgaag tccgccggac ggactcatct
     3541 cggcagggtc gaatcgccgc ggtgagtctg tccggggaat tccgtcgtgt cctgcggaaa
     3601 tgagcccgcc aggagattct ccatccatga cgagcgtcgt ccgccggacc ccctttcgtc
     3661 cacttacggc ggccttcttt ctcggcctgc ctgtgatact tgccgccggc tgcggatacg
     3721 agccggcgat atctgcacgc gacgttgccg ccgctcgtgc gacgaaagtc gcgggcgcgg
     3781 caatcacgcc tgagccgtcg cgcgccgcgg cgaaacgtcc ggcgccaggg tccgctgctg
     3841 aggatggtca ttcgacgatc cggttccgag aggtcgagcg ctcggcgggc attacgttta
     3901 cccacgtcag cggcaacagt ccagacaaac actatcccac ggccaatggc tcgggcgttg
     3961 ccatgttcga ctacgatggc gacggccgtc ttgacctcta ctttgccaca actcgcaatt
     4021 tgcccctgca tgccccgaca acctctcagg ggaacaagtt gtatcgcaac cagggagatg
     4081 gatcgttcga ggacgtcacc gatcgcgccg gcgtcggctt tcggggcttc tgccacggcg
     4141 tggcggttgg cgatgtcgac aacaacggct acccggacct ttacctgacc aactatgggc
     4201 cgaacgtact gtacctcaac aacggggacg gcacttttcg caccgcccga gacttcggag
     4261 cggagtgccg atcttggtcc acggcggcgg cattcctcga ctatgatggc gacggttggc
     4321 tcgacctgta tgtctcgagc tacggccact gggtcttcga cgagccgcac ccgttttgcg
     4381 ggaatgaggc ccagggtgtg cgcatttact gttcacccat cacgattccg cccgcgccgc
     4441 attttctttt ccgaaaccga ggcgatggga ctttcgagga cgtgacgcag cgcgccggcg
     4501 tgctgcgccg cgacgggcgt ggcatgggag tcgtggccgc cgacgtcaac cgcgacgggc
     4561 gcgtggacct ctatgtcgcc aacgatatgt gccctcattt tctcttcctc aatcgagggg
     4621 acggcacgtt cgaggacgtc accgatactt caggtgccgc actcactgag tcgggtcatg
     4681 tccagggtgg gatgggcgtc gacgtcgagg acctggacgg agacggttac cccgagctgt
     4741 tcgtcaccaa ttttgacgga gaatacaaca cgatccaccg cacgaccgac ggtcgaattt
     4801 tcgaggatgt gagcgcaagg tcgggtatcg tgccggatag cctggccgac gtcggttggg
     4861 gctgcgccct tctggacctg gacaacgacg gatggcccga catgatcacc gtcaacggcc
     4921 acatctacga caatctggca caggttggcc gcgacggcga atatgcgcaa cgccccaaga
     4981 tatggcgcaa ccgtgggcga ggccggttca aactggtcgc ggatgccggg ccgttctttg
     5041 acgttggtca cgccgcgcgt ggggccgcgt ttggcgacct ggataacgac ggcgatatcg
     5101 acgtcgtcat caaccggatg gacagccggc cggcggtatt attgaatgag tcgcaacccg
     5161 cccactggat ccgcctcgag ctagtcggga gccggtccaa ccgctcggcg atcggcgcct
     5221 cggtcgcggt ccatgccggc gatcgcgtcc tgcaccgcca ggtcaaagga gggggtagct
     5281 acgcatcggc caacgacccc aggctcctgg taggtttggg gggggccgac cgagtggatc
     5341 gggtcgagat ccgttggccc agcgggcgga gtacgacgct gaccaacctt gcgctcgccc
     5401 gcacccaccg ggtcgtcgag cccgacggac ttgtgggcag cgatgctccc tccgcgaccg
     5461 atgggagacg gcggtgatga gttcaaccat tgactcgggg cggaagcgga tcatccgcta
     5521 cgtcttgacc gggctgacct tggccttggg ggtcggcgga ctggcctggg gcgtccggca
     5581 gtcgttgcgg tcgaggtcgt cgctggacga agcgatcggg ctagcggagg ccggcagact
     5641 ggtagaagct gaggcgaagg ttcgtgcccg gctggcgacg gatcccgatg ccgccgcggc
     5701 gcacctgctc ttggcccaga tcatcctcaa gcggccggac caaccctccc cgccgtccga
     5761 ccgttgcgcg tcgccctccg gacgggaggc gctcgatcat ctcgatcggg tccacgctca
     5821 gaatcccgcc atggatgtta tcttccatct ctgtcgaggc gacgccctca aacgcctgat
     5881 gcgatttgat gacgccgagg cggactggct tgaagcattg cgaatcgaca cgaccgcgcc
     5941 ggaggtcggt ttcaacctcc tggatctgta ttacttcgaa gggagagaag aagagtcacg
     6001 ccgcctggcg ttgcgactct accgggtcga gcctgaccca cacgatcgcg cgacattgct
     6061 cgtggagctg ctgcggctag acgtgcgtcc cccggcacct ggctcgatcg agaaactatt
     6121 ggaaccggta gtccggcaca atccgggcga cctgcacact ggcgtggcga tgggcttggc
     6181 ccttacccgt gccaacaaag tcgacatggg tatcgaccaa ctccggcgcg tggtgcgaac
     6241 gcacccggat agcgtcgagg cctgggacgg cctcctgacg ggcctcgatg agtcgggcca
     6301 ggtggatgtc attgaggagg agctggagcg cctgcctgca ggcctctccg atgcgccgcg
     6361 tctactcaag cataaggcga ggatcgccca ggatggcaat cactggagtg aggccgtcgg
     6421 cctcttccgc cgtgcccgcg ccaatgagcc ctccaaccgg gtcgtcgagt accgtctcag
     6481 ccgagccctg cgtcatgcag gcgagacgga tgaggccaat cgattagagc agcgcgttcg
     6541 ccgccgtgag atcgctctcc aggagctgcg tcccctcttc gacaaggcca cggcgacacc
     6601 cgatctggga acacgtccgt acccggagct ttaccatcaa atcgccgaag tgcgagagcg
     6661 catgcagttg cccgaggagg caatggcctg gcaccgcctg gtattgggaa acgaccctaa
     6721 aaacgaagtc agtctcgccg cccttgcccg gttggggaat gagagcgact cactctgatc
     6781 gacaacgtct aacacggcgt gcctccagga cgctggctca taccgcccga ttggtatcga
     6841 ccgtcgacct ttaacaaggg atccagccaa tgtcgtttct cgatgatcga tccccgacat
     6901 ccttgggggc gtcggaaagc cggcggcaac cggtggatgc gggactggcc aacgaatcac
     6961 ccacgggacc gacgaaggcg ccgtcatcac cactacggag ctggttgctc gtcctcgcag
     7021 ccg
//
LOCUS       DATAQL010000945         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1013377, whole genome shotgun sequence.
ACCESSION   DATAQL010000945 DATAQL010000000
VERSION     DATAQL010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013377"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..627)
                     /locus_tag="VHS97_16435"
     CDS             complement(31..627)
                     /locus_tag="VHS97_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449843.1"
                     /translation="MLNHRLSPMVMAATAMALSLSLSSTARGQGGFDPTALIAAQREA
                     MAPLAYMDGVWRGPAWTILESGEKHTFTQTERIGPFLDGSVKVIEGRGYNDDSKIRFN
                     AFGIISYSPEKKGYVLHSHAMGRVGDFALKPTTDGFVWEIPAGPMTFRYTAVIKDGTW
                     REVGDRILPGKEPARFFEMNLKRLRDTDWPSAGAIPPK"
     gene            1006..1449
                     /locus_tag="VHS97_16440"
     CDS             1006..1449
                     /locus_tag="VHS97_16440"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3449844.1"
                     /translation="MNKKYIVRLTDQERDELVAVVKKLKGTSEKVRRAQILLKADADG
                     PNWTDHWIAEAFGCRTQTVENDRRRLVEHGFREALDRRSRKTPPVEKLLNGEQEARII
                     ATRLGSPPPGYANWTLRLLARKVVELGIVESVSHETVRRTLKKTG"
     gene            1446..1592
                     /locus_tag="VHS97_16445"
     CDS             1446..1592
                     /locus_tag="VHS97_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449845.1"
                     /translation="MTNRKIQYWVIPPVADGEFVAHMEEVLETYARPYDPKRPVLCMD
                     EQSL"
     gene            complement(1593..2024)
                     /locus_tag="VHS97_16450"
     CDS             complement(1593..2024)
                     /locus_tag="VHS97_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449846.1"
                     /translation="MEAGSGIFDHDLDAQPRRRIPTREDPPYEYQRLVVNPFLTVLIW
                     IIFSGLHRGIEPRLTAAVFVAVLVVFVLGLFLVQFHCLDCGATGWLVRYQRHACPKVV
                     ARWRSRVVRRFHGPRLRIQLIAWLIVTMAALLLGALALGSR"
     gene            2533..3864
                     /locus_tag="VHS97_16455"
     CDS             2533..3864
                     /locus_tag="VHS97_16455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3449847.1"
                     /translation="MPTQPGARLRNRQYLTVALMVVGYAGYYLCRSNLSTTMPLITLD
                     LVGRGFDAAMVRSWLGWAVSLGTIGYAIGKFAAGTLTDLLGGRRTYLVGMAGAVVCTV
                     LFAIGGSLPLLSLTWFANRLIQSFGWPGMIKITSRWFSYSSYNAVMGVISLSFLFGDA
                     AARFFMGQLIKAGLGWRTVFWVAAGVLAVVWVVNAILIRESPGELGLPEPQTNPDNLF
                     GALGEDPEPESAGPLVATLLRSPAFWIVCFMSLGLTLLRETFNNWTPTYLVEAVGLTS
                     GSGAQMSSLFPLFGGVSVLLAGFLGDRLGRVGRAAIILAGLLLGGLALGLLGSVSFAG
                     RSGEALVLVSLVAFLLIGPYSYLAGAISLDFGGKRGGATACGIIDGFGYLFGGVIAGK
                     VVSALSGSLGWQGVFQMLAVVAFLTGVTAAGFLVTQWQRGAKPSESPAKSP"
     gene            3929..>4363
                     /locus_tag="VHS97_16460"
     CDS             3929..>4363
                     /locus_tag="VHS97_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3449848.1"
                     /translation="MKIVDEIIDLFHQRGDTAYHGEAVSQTEHALQSAALAERQGAAD
                     NLVIAALLHDVGHLLGGQDEDLAVKGVDGQHEAAAYAWLSKYFGPEVTEPVRLHVAAK
                     RYLCAKNATYLQGLSPASQLSLEVQGGPMTPLERAQFETNPHY"
BASE COUNT          844 a         1394 c         1268 g          857 t
ORIGIN      
        1 caccccgctg caattacgct ggcaaccgct tcacttgggc gggatcgccc ccgcggacgg
       61 ccaatccgta tcgcgaaggc gcttgagatt catctcgaag aaccgggccg gttccttgcc
      121 cggcaagatc cggtccccca cttcccgcca agtgccgtcc ttgattaccg ccgtgtaacg
      181 gaatgtcatt ggcccggcgg gaatctccca aacgaagccg tcggtagtcg gcttgagtgc
      241 aaaatcgccc actcgcccca tcgcgtggga gtgcaggaca tagcctttct tctctgggct
      301 ataggaaatg attccgaacg cattgaagcg gatcttactg tcgtcgttat aacccctccc
      361 ctcgattacc ttgaccgacc catccaggaa cgggccaatc cgttcggtct gcgtgaaggt
      421 gtgcttctcg cccgactcta ggatcgtcca cgccggcccg cgccagactc cgtccatgta
      481 ggcaagagga gccatggcct ctcgctgcgc tgcaatcagt gcggtgggat caaatccgcc
      541 ttgtcctcgc gccgtggagg acagtgaaag cgacagtgcc atcgccgtcg ccgccatgac
      601 cattgggctc aatcgatggt tcaacatctt ctcgcgctcc aaggcttctc gttcaaacaa
      661 accgccctcg cgacagtgcg aattcacgtc ggtaatcgta accggtaaat ccaaccgcgt
      721 cagcgggctt gccgctcaca agggcgccaa caggtaaaga acaggcaagt acagctttgc
      781 agcatcgagg acgggcgttc gatgacgcac tgatgcggtc gtgttttctg gaacgtccgt
      841 cgcggacgac acttccacct tgcggagacc aagggctcga ccttctggct gccgcccaca
      901 ccacaggcgc gcgggacatc cctgccctgt gctctgtcac tgctaaaagc tgggctcggt
      961 gaaaccgtcg cgacggggat gatgcgagga acacggaggt ttcctatgaa taagaagtat
     1021 atcgtccggc tcaccgatca ggagcgcgat gagttggtcg ctgtggtcaa gaagttgaag
     1081 ggtacgagcg agaaggttcg gcgcgcccag atcctgttga aagccgacgc agacggcccg
     1141 aactggaccg atcattggat cgcagaggcg ttcggatgcc gaacacaaac cgttgagaac
     1201 gatcgtcggc gcctcgtgga gcacggcttt cgagaggcgc tcgatcgtcg cagccggaag
     1261 acaccaccgg tcgagaagct actgaacggc gaacaggagg ccaggatcat cgccacgcga
     1321 ctcgggtcac cgcccccggg ttatgccaac tggacgctgc gactcctggc gcggaaggtg
     1381 gtggagttgg ggatcgtcga gtcggtcagt cacgagacgg tgcggcgcac tctcaaaaaa
     1441 acgggatgac gaaccgcaag atccagtact gggtcatccc tccggtggcc gacggcgaat
     1501 tcgtagccca tatggaagag gtgctggaga cctacgcgag gccgtacgat ccgaagcggc
     1561 cggtcctctg catggacgag cagagtttgt gattagcgtg atccaagcgc cagcgcgccg
     1621 agcagaagcg ccgccatcgt gacgatcagc caggcgatga gctgtattct gagcctcggc
     1681 ccgtgaaatc gccggacgac tcgacttcgc caccgtgcca cgaccttggg acaggcgtga
     1741 cgttgatagc ggacgagcca tccggtcgcg ccgcaatcca ggcaatgaaa ctgaaccaag
     1801 aagagtccca agacaaaaac gacgagcacc gcgacgaaca ctgccgctgt gagcctgggc
     1861 tcgatgccgc gatgtagtcc cgagaaaatg atccaaatca ggaccgtgag gaatgggttc
     1921 acgacgagcc gttgatattc gtacggggga tcctcgcgag tcggaatccg ccggcgcggc
     1981 tgggcgtcga gatcgtgatc aaagattccg ctcccggctt ccacgacggc tccttcgcct
     2041 cgcagtgaac gaatcacgga aaactgcttt gtccttttcc ctctcattaa tggtaaacat
     2101 tcctgatgta cggacgcgat aggctgaacg atcggtcata cgcattggca aaccaggaaa
     2161 gcctagctga cgggagcagg ctcgcctccc cgggcggttc cttgatgaag taccggtgcc
     2221 agagacagtg tggaaagcta tccagtccgg attggtctat ttcgctttgc gatcgccagt
     2281 ctccgacacc gcgatggacc gaccgaccca ttccccacct ggtgactcag tcccaatggc
     2341 gaagacgtcc gcgaatagac cacaacgcgc aaccaacata cgcccatcgg ccggacactt
     2401 ctggtgtttc atcctgaggg caatgcacag tctgccgatc tcgtcgtccc ttcggcgacc
     2461 gtctcctcga gccccagccc gcgaatcccg ctccaacgac gcgccgatct ttttgagcgt
     2521 gagggtcatt agatgccaac ccagccaggc gcgcgactga gaaaccgcca gtacctcacc
     2581 gtagcgctca tggtcgtggg ttacgcgggc tattacctct gccgctccaa tctctcgacc
     2641 accatgcccc ttatcaccct tgatctggtg gggcgaggct tcgacgcggc catggtgcga
     2701 agctggctgg gatgggccgt atcgctcggc acaatcggct acgccatcgg caaattcgcc
     2761 gccggcaccc tcaccgacct tttgggcgga cgccgcacct accttgtcgg catggccggc
     2821 gccgtcgttt gcacggtcct ctttgccatc ggcggctcgt tgcccctcct ttcactcacc
     2881 tggttcgcca accggttgat ccagtcattc ggctggccgg gaatgatcaa gatcacttca
     2941 cgctggttct catattcctc ttacaatgcc gtgatgggtg tcatcagcct gagctttctg
     3001 tttggagacg ccgctgcccg gtttttcatg ggccagttga tcaaggccgg tctgggctgg
     3061 cgcaccgtgt tctgggtagc cgccggagtg ctggcggttg tctgggtcgt aaacgccatc
     3121 ttgatccgcg aatctcctgg cgagttgggt ctacccgagc cacaaacaaa ccccgacaac
     3181 ctgttcggcg ccctgggaga agatcccgag cccgagtcgg ccggaccact cgtggcaacc
     3241 ctcttgcgca gccccgcgtt ctggatcgtg tgcttcatgt cactcggttt gacgttatta
     3301 agagagacgt tcaacaactg gacgccaacc tacctggtgg aagccgtcgg cctcaccagt
     3361 ggaagcggag cgcagatgag cagcctcttt ccattgtttg gaggtgtctc tgtgctcttg
     3421 gccggtttcc tgggcgaccg gctcggtcgc gtcggccgcg ccgcaatcat cctggccggc
     3481 ctcttactcg gcggcctggc acttggcctc ttgggctccg tcagtttcgc cggccgctcg
     3541 ggcgaggccc tcgtcctggt gtcgctggtg gcgttcctct tgatcggccc gtactcttat
     3601 ctcgcggggg ccatctcgct tgactttggc ggcaagcgag gcggcgcaac cgcctgcggc
     3661 atcatcgacg gctttggcta tctgttcggc ggcgtcattg ccggcaaggt cgtctcggcc
     3721 ctttcgggtt cgttgggatg gcagggcgtc ttccagatgc tcgccgtcgt cgcattcttg
     3781 accggcgtga cggccgccgg ctttctggtc actcaatggc agcgcggggc taaaccctcc
     3841 gagtcgccgg caaagtcccc ctgagtcacc gacaaccgag gatcaacctc ggagagtgac
     3901 gaagtcgcgc atataaggac tctttctcat gaagatcgtc gacgaaatca ttgacctgtt
     3961 tcaccagcgc ggcgacaccg cctatcacgg cgaagccgtt tcccaaaccg aacacgcgct
     4021 ccagtcggcc gcgctcgccg aacgacaagg agcggccgat aacctggtga tcgccgcact
     4081 cctgcacgac gtcggtcacc tcttgggcgg tcaggatgaa gacctcgccg tcaaaggcgt
     4141 cgacggccag catgaagcgg cggcctacgc ctggctctcg aaatacttcg gccccgaagt
     4201 caccgaaccc gtccgcctcc atgtcgctgc caaacgctat ctctgcgcca agaatgccac
     4261 ctacttgcaa gggctctccc ccgcgtcgca gctcagcctc gaagtccagg gcggccccat
     4321 gactcccctg gaacgcgccc aattcgagac caacccccac tat
//
LOCUS       DATAQL010000946         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4829461, whole genome shotgun sequence.
ACCESSION   DATAQL010000946 DATAQL010000000
VERSION     DATAQL010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4829461"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VHS97_16465"
     CDS             complement(<1..608)
                     /locus_tag="VHS97_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX3449849.1"
                     /translation="MGVFPSVLLAFLFLAGGDDRGSAPPKFAFFEPVSPPRAIEVMAH
                     RGAMRYAPENTARALELSIADAVEWVEVDVRLTKDGQHVLFHDDQLGDKTDGTGLLRD
                     RTLAELRAIDAGTKFAPRFASARILTLAEGLELARGRVNLYLDCKDVDPAKLADDVIA
                     ARMEHQVVIYDTPQVLTAIRAAASRELGLMTKWRPRFGFTPWV"
     gene            complement(660..1922)
                     /locus_tag="VHS97_16470"
     CDS             complement(660..1922)
                     /locus_tag="VHS97_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449850.1"
                     /translation="MAGTWAVLGLVVGFRRDSYLRGLSIAVTYITVCLETFVIISLSH
                     IRSKRTGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRAQCLNNLKQLGLALANYE
                     SSNACYPMAAFSQTNPKTGPGDQGFSCMVGLLSYMEQAPLYSSFNTNFSYRCDANSTV
                     SGTGLSVLWCPSDSAIIGINFVEPAGTHGKTFPQTFYFSSYAGCYGEWAGLWTGVGKT
                     LGAVSGTPALQQQNGAIVSQGIDPLQVSLAAGPGIPSLNRGGVVRIASVTDGTSNSIA
                     FGEHAHGLLSMTDPERSFFKWNWWVSGNYGDTAFTTFYPINVQKKYPDFNNADKAGVV
                     SSTEGGAFLNAATSFHPGGANFAFCDGSVRFLKDTISTWQLDRTGTPIGASWNAQTYV
                     WTLGTAQPGVYQALGSINGGEVMSADSY"
     gene            complement(2480..>2700)
                     /locus_tag="VHS97_16475"
     CDS             complement(2480..>2700)
                     /locus_tag="VHS97_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449851.1"
                     /translation="RLLMWLGMVGPLIALQVPVGRREEPNVVSSRDRQVIADLIRELD
                     RLDATARIDAVLALTSIGMKYPSTSVPG"
BASE COUNT          558 a          806 c          829 g          507 t
ORIGIN      
        1 acccatggcg tgaagccaaa gcgagggcgc cactttgtca tcagccctag ttcccggctc
       61 gcggccgcgc ggatcgccgt gagcacctgg ggggtgtcgt agatgacgac ctggtgctcc
      121 atcctggctg caatcacgtc gtcggcgagt ttggcggggt caacgtcctt gcaatcgaga
      181 tagaggttga cccgacctcg ggccagctcg agcccttcgg ccagcgtgag gatgcgcgcg
      241 ctagcaaatc tgggggcgaa cttggtgccg gcatcgatgg cccgaagctc ggccagcgtt
      301 cgatcgcgta acagccccgt gccgtctgtc ttgtcgccga gttgatcgtc gtggaagagc
      361 acgtgctgac catccttggt gagacggacg tcgacctcga cccattcgac cgcgtcggcg
      421 atcgaaagct ccagggctcg agcggtgttt tccggcgcat agcgcatggc gccgcgatgg
      481 gccatgacct ctatggcgcg cggcggactg accggctcga agaacgcgaa cttcggggga
      541 gcgctcccgc ggtcatcccc gccggcaagg aataaaaagg ccaggagaac gcttgggaat
      601 acgcccatgg ggctgcacaa acccggttgg ggtcgcctct cttattctcg gtttgattgt
      661 tagtaactgt cggcgctcat gacctcgcct ccgttgatcg agcccagggc ctggtaaacg
      721 ccgggctggg ctgtgcccaa agtccaaacg taggtctggg cgttccacga cgcgccgatg
      781 ggggtgccgg tgcgatcgag ctgccaggta ctgatcgtgt ccttaaggaa cctgaccgag
      841 ccatcgcaaa aggcgaaatt cgcgccgcct gggtggaagc tcgtcgccgc gttcagaaat
      901 gcgccgcctt cggtcgagct gacgacgccg gccttgtcag cattgttgaa gtccggatac
      961 tttttctgaa cattgatcgg gtagaaagtg gtaaacgctg tgtcgccata gttcccggaa
     1021 acccaccaat tccacttgaa gaatgaccgc tcggggtcgg tcatggacag caggccatgg
     1081 gcgtgttcac cgaaagcgat gctattgctc gtgccgtcgg tgacgctggc gatccgcacc
     1141 acgccgcctc gattgagcga cgggatgccc gggcccgcgg ccagcgatac ctgaagcggg
     1201 tcaatgccct gggagacaat cgccccattt tgctgttgca aggccggcgt gcccgagacc
     1261 gcccccaggg ttttgccgac gccagtccac aaaccggccc actcgccgta gcagcccgca
     1321 tagctgctga agtagaaagt ctgcggaaag gtcttgccgt gcgttcctgc cggctcgacg
     1381 aagttgatcc cgatgatggc cgaatcactt gggcaccaaa gcaccgagag gccagtgcca
     1441 gagaccgtcg agttcgcgtc acaccgatag ctaaagttcg tgttgaagga cgaatagagg
     1501 ggggcttgtt ccatatacga cagcagcccg accatgcaac taaacccctg gtcaccaggg
     1561 cctgtcttgg ggttggtctg ggaaaacgcc gccatcgggt agcaggcgtt gctgctctca
     1621 tagttggcca gcgccaagcc cagttgtttc aagttgttga ggcactgcgc gcgccgagcc
     1681 gcctcgcgcg cggcctggac ggccggcaag agcaaggcga tcaaaacggc aatgatggcg
     1741 attaccacca gcaattcgat gagcgtgaac cccgttctct ttgaacggat atgactcaat
     1801 gatataatca cgaatgtctc caagcaaacg gtgatgtagg tgacggctat ggaaaggccg
     1861 cgaagataac tgtctcgccg gaagccgaca accagaccca gcacggccca agtgccagcc
     1921 acgccgcgcg aggcaattcc tgatctggac gaagtcaggt gcttctctcg ccacgggcct
     1981 tcttcgccga tttaggcaat ttcgccttct caatgacggc gccggaccgg gccttgatcg
     2041 agatcgatcc gaccgagggt tgatccgaat cctggttgct gcaccccgat atcatggagg
     2101 cgagcaacgc cgcggcaatc aaacagacag gaagtctcat cattcaaacg ggtgcgatcg
     2161 ggctatcccc atccgccggc gagcgacacg ctgccgaaag caaggggaga atcgcaggat
     2221 tctagttatc gtaggcgaat aaatggagta gtagccgtga agtcctaatt aattcgctgt
     2281 atttgaggta ccatgatcag aggcaaagat cggatggggc ccaacccccg ccccttatct
     2341 cctttgcgcc gcctgcgcaa ctcctttccg cagatctcgc cggcaatgac tccaaactcc
     2401 catcagggaa acgagggacc gccgggaact ggctcgatga agtgcctgga ttagcggaac
     2461 ccaccgcgca tgtaggccat caccctggga cggaggtaga tgggtacttc attccgatcg
     2521 atgtgagggc aaggacggcg tcgatgcgag ccgtcgcgtc cagcctgtcc agttctcgaa
     2581 tcaagtcggc gatgacctgc cggtcgcggc tcgagacaac attaggctcc tcgcgccttc
     2641 ccacggggac ctgaagggcg atgagcggcc cgaccatgcc gagccacatg agaagtctca
//
LOCUS       DATAQL010000947         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2693850, whole genome shotgun sequence.
ACCESSION   DATAQL010000947 DATAQL010000000
VERSION     DATAQL010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693850"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /locus_tag="VHS97_16480"
     CDS             <1..1656
                     /locus_tag="VHS97_16480"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX3449852.1"
                     /translation="RVGALPIINRLLERMRLQEFLHAYLPKADRRCRIAPSIGISLLL
                     KNVLLSREPLYGIGEWAARFAPEALGFAGTQLPSLNDDRMGRCLDRLFQSDITSMVLA
                     LMTHVVDEFQVDLDELHNDSTTITFHGAYADAAEEEKRGKQTRMAITWGYNKDHRPDL
                     KQLLYILTVAKDGAVPLYFQVASGNVTDDQTHRATWDLLCRLTGRRDFLYVADCKLAS
                     TENMAHIHQRQGRFLTVLPRTRSEDRTFRDLLSSGQIRWRHIHDKRNDDGKIVDQYSI
                     SEPALLSAEGYRLVWYHSTRKAEQDAHARHQQVERALNELSELRLKLSSPRTRYRQET
                     KVADAVQEILRARGAENWIVTAIKERTEEKYRQDRRGRPNDLTRYVRETSTRFELTYH
                     IDNARLAAETCGDGVFPLITNDDSLSYLEMLLAYKSQPALEKRFSHLKTDFEVAPVYL
                     KEASRIQALLCVYFLVLLAESLLERELRRAMDREAIESLPLYSEGRKCRHPTARRLID
                     LFEDVQRHSLRVGRQPSMIFTTELSRLQRRVLRLLGMAKVYDA"
BASE COUNT          415 a          573 c          517 g          387 t
ORIGIN      
        1 cgcgtcggcg ctctgccgat catcaatcga cttctggaac gaatgcgcct ccaggaattc
       61 ctccatgcct atctccccaa agccgatcga cgctgccgga tcgctccctc gatcggtatc
      121 tctctcctgc tcaagaacgt gctcctctcc cgcgagccgt tgtacggtat cggagaatgg
      181 gcggcgcggt ttgctcccga agcactcggg ttcgccggca cccaattgcc atccctcaac
      241 gacgatcgta tgggacgctg tctcgatcgc ttgttccaaa gcgacatcac ctcgatggtt
      301 ttggcgctca tgacccatgt ggtcgacgag ttccaagtgg acctcgatga gctgcacaac
      361 gactccacca cgatcacctt ccacggtgcc tatgctgacg cggctgaaga agaaaagcgg
      421 ggcaaacaga ctcggatggc aatcacatgg ggttacaaca aggaccatcg gcctgacctc
      481 aagcaactgc tttacatcct cacagtggcc aaggacggcg cggttccgtt gtacttccag
      541 gttgccagcg gcaacgtgac ggacgaccag acccaccgtg ctacctggga cctcctctgt
      601 cgcctgaccg gaagacgaga tttcctctat gtcgcagact gcaagctggc tagcaccgag
      661 aacatggccc acatccatca acgccaaggc cgattcctca ccgtactgcc gcggactcgg
      721 tccgaggacc gtaccttccg agacttgctc agcagtggcc agatccggtg gcggcacatc
      781 catgacaaac gaaatgacga tggcaagatc gtggaccaat actcaatctc tgagccggct
      841 ttgctgagcg ccgaaggata ccgactggtc tggtaccaca gcacgcgcaa agccgagcag
      901 gatgcccatg ctcgtcatca gcaagtcgag cgagccttga acgaattgtc agagctgcgc
      961 ttgaagctct catctccacg cacgcgctat cggcaagaaa cgaaggtcgc ggacgccgtc
     1021 caggaaatcc tccgtgcccg tggcgctgag aattggatcg tcacggcgat caaggagcga
     1081 accgaggaga aataccgtca ggaccgccgg ggccgaccga atgatctaac gcggtacgta
     1141 agggagacaa gcactcgctt cgagctgacg tatcacatcg ataatgctcg actcgccgcg
     1201 gaaacgtgtg gcgacggtgt ctttcccttg atcactaacg acgattcgct gtcgtatctg
     1261 gaaatgttac tggcctacaa gagccaaccg gccctcgaaa aacggttctc gcacttgaag
     1321 accgacttcg aggttgcccc ggtttatctc aaggaggcca gccggattca ggcgttactt
     1381 tgtgtctact ttctggtgct cctagccgag tcgctacttg aacgcgagct gcgccgcgcc
     1441 atggaccggg aggcgatcga aagcctgcct ctctactccg agggtcgcaa gtgtcgccat
     1501 cccacggccc gtcggctgat cgacctgttc gaggatgtac aacgtcactc gcttcgcgtt
     1561 gggcgacaac cgtcgatgat cttcacgacc gagttaagcc gactgcaacg tcgggtgttg
     1621 cgtctactcg gaatggcgaa agtatacgac gcctgatttg gtcagccagc atgccgtaga
     1681 gtcgagcgta agggcccgca atcgcataaa agcttcggcg acttggcacc cgacgaaatt
     1741 ccaggataat caactgcgga tgtgcggaac gtcagctagt gctcagtcaa ttttgaattt
     1801 tcgggtacag caatttcagt ttggtgcgcg cgtcgtcaat tcggaattgc caatcgacgc
     1861 cgcgttgttt ctcgttgctg ttccgcgacc aa
//
LOCUS       DATAQL010000948         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3149759, whole genome shotgun sequence.
ACCESSION   DATAQL010000948 DATAQL010000000
VERSION     DATAQL010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149759"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VHS97_16485"
     CDS             <1..543
                     /locus_tag="VHS97_16485"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX3449853.1"
                     /translation="GGAHWRIGEDQKPGKFGAGAWFQTGKFHGFDGRPLTGANGIYLF
                     GSQRLYYENPAVSNNGLLSWVQFGATNSDIVLTHRYFGFGLTYFGLLPSRDEDSCGFG
                     LAWGTMTRDPQAGLVFFGNDHLRTNQLGANEVILTWYYQMTVREGIYIQPNLSYIPDP
                     AQHPGIPGSFNFTMRGIVLF"
     gene            complement(582..1553)
                     /locus_tag="VHS97_16490"
     CDS             complement(582..1553)
                     /locus_tag="VHS97_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449854.1"
                     /translation="MRTKFDGTRSRAAFTLIELLVVIAIIAVLVALIMPAVQSARESA
                     NRAKCQNNLKQLNLAAQEYHDAYNSFPAGWYCSVPQYDANGNLISGDINCATPGTPYQ
                     NYMWNGLIGLFLKMEQNNLYSEINFNLATTMPDNATAIRRTVDGFVCPSNRRPTTVAT
                     GTGSQTSSQLGPSDYRGNMAAGMVPPNSQSGCPTQDPTNPSCLYYDNGVTYQNSTVNM
                     ADITDGSSTTIILGESIYPQGVWSQATTSVVRTNIDRNINKPIIVNVNGANVNYWTYW
                     ASKHPSAVNFAYCDGSVRPVTSQINKLVLIKLCTRNGGEAISSDEMK"
     gene            complement(1747..2379)
                     /locus_tag="VHS97_16495"
     CDS             complement(1747..2379)
                     /locus_tag="VHS97_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX3449855.1"
                     /translation="MSIGARDALDASCLVLNKYYMAVHVVSVRRAFCLLFKELAEVIT
                     IDDGRYASFDFASWREVSQARVLFREPDDDFIRTVHFEIQVPRIIRLLTYDRLPRQKV
                     KFNRRNLFARDGNRCQYCGKKFATDALSLDHVVPRVRGGKGSWDNIVCACLRCNVRKG
                     GRTPREAGMKLIREPVEPKTSPVVTLKLSHRKYKSWRAFLDNAYWSVELE"
     gene            complement(2545..>3132)
                     /locus_tag="VHS97_16500"
     CDS             complement(2545..>3132)
                     /locus_tag="VHS97_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX3449856.1"
                     /translation="RCIRWAFEFTRRRNNPKGKMLTLVAKTNVLTFGHDLWWRAFQEV
                     AREYPDVKADYNHVDACCMWMVKNPEYYDVIVTTNMFGDIITDLGAVLQGGMGLAASA
                     NLNPDRTAPSMFEPVHGSAPDIAGRGIANPLAAILSAAMMLDHMNLPRAAGRIRAAIR
                     QVLSQPAPRTPDLGGDATTTSVGRAVREALAGDVS"
BASE COUNT          604 a          885 c          960 g          683 t
ORIGIN      
        1 ggaggcgccc actggaggat cggtgaagac caaaagccgg gcaagtttgg cgcaggcgct
       61 tggtttcaga cgggcaagtt ccacggattt gatggtcggc cgctcacggg tgccaacggc
      121 atctatctct tcggttcgca gcggctgtat tacgagaatc ccgctgtcag caacaatggc
      181 cttctgtcat gggttcagtt tggcgcgacc aattcggata ttgttttgac gcaccgctat
      241 ttcggatttg ggctcactta cttcggcctg ttgcccagcc gcgatgagga ttcgtgcggc
      301 tttggcctgg cctggggcac gatgacgcgg gacccgcaag ccggcctcgt cttcttcgga
      361 aacgaccatc tccgcacgaa ccaactggga gccaacgagg tcatcctaac ctggtactac
      421 caaatgacag tgcgtgaagg aatctatatt caacctaatt tgagctacat tcccgacccg
      481 gcacagcatc cagggatccc cggctcattc aactttacca tgcgcgggat cgtgctcttt
      541 tgatcggccg gcgccgtcgc cctagttgtc ggtagccgcg ttcacttcat ctcgtcgcta
      601 gagatcgcct cgccgccgtt acgggtgcaa agcttgatca aaaccagctt gttgatttgt
      661 gaagtcacag gccgcacgct gccgtcacag taggcaaaat tgaccgcact cgggtgtttg
      721 ctcgcccaat acgtccagta attgacgttg gcgccattga cgttgacgat gattggcttg
      781 ttgatatttc gatcgatatt ggtccgaacg acggaggtcg tggcctgcga ccagactccc
      841 tgtgggtaga tgctctcgcc gaggatgatc gtggtggacg acccgtcggt gatgtcggcc
      901 atgttgacag tggagttctg gtacgtgact ccgttgtcgt aatagaggca agagggattg
      961 gtggggtctt gggtggggca gccactttgg ctattcggcg ggaccatgcc ggcggccatg
     1021 ttaccgcggt aatcggatgg accgagctgg gagcttgtct gagagccagt cccagtggca
     1081 accgttgtcg gccggcggtt cgacgggcag acaaagccgt cgaccgttcg ccggatggcg
     1141 gtcgcgttgt cgggcatcgt ggtcgccagg ttgaagttga tttcgctgta caggttgttc
     1201 tgctccatct tcaagaacag tccgatcagg ccgttccaca tgtaattttg atagggtgtt
     1261 cccggcgtcg cgcagttgat gtctccgctg atcaaattgc cgttggcgtc gtactgcggc
     1321 acggaacagt accaaccggc tggaaaagag ttgtacgcgt cgtggtattc ctgagcggcg
     1381 aggttcagtt gcttcaggtt gttttggcac ttcgctcgat tggcggattc gcgcgccgac
     1441 tggaccgcgg gcatgatcag ggcgaccaag acagcgatga tggcaatgac cacgagcagc
     1501 tcgatcaacg taaaggccgc tctcgaccgt gtaccgtcaa atttcgtgcg catcaaagtt
     1561 ccctcgaaca tgaggctacc agactaccgc gcggcgctgc gggactctct ctaatccaaa
     1621 ctacacgatt cgatccattg ttgccatctc gctggcgcgt tttctttgag ggagcacaga
     1681 agcggtggct ttgtatcgcc ggctcacctc gcctgggaca ccccactcag atcaggagtc
     1741 atcgcgtcac tcaagctcga ccgaccagta ggcgttgtca agaaacgctc gccagctctt
     1801 gtacttacga tgggacagct tgagagtgac gacggggctc gttttcggtt cgacgggctc
     1861 gcgaatcagc ttcattccag cttcgcgagg cgtccgtccc cccttacgga cattgcagcg
     1921 caagcacgcg cagacgatat tgtcccagct tcctttgccg ccacggacac gtggcaccac
     1981 gtggtcgagg ctgagggcgt cggtcgcaaa cttcttgccg caatactggc aacggttgcc
     2041 gtcacgagca aacaagttcc tgcgattaaa cttgaccttt tggcgaggta ggcgatcgta
     2101 cgtcaacagc cggatgatcc gaggtacttg gatctcgaaa tgaacggttc gaatgaaatc
     2161 gtcgtcgggc tcgcgaaaca gcacgcgggc ctgagagact tcccgccagc tcgcgaagtc
     2221 gaacgacgca tagcggccgt catcgatcgt gatgacctcg gccagttcct tgaataacag
     2281 gcaaaatgca cggcggaccg acacgacgtg tacagccatg tagtatttgt tcagcacgag
     2341 gcaactggca tccagggcat ctctggcacc gatgctcatg aagaggggcc tccttgcgtc
     2401 gcggtcagtc gtcagtggtc attggtcgga gctgccgctc tagattggcg agagaaggaa
     2461 cggacgcgat cggatcgcgt ttgagatcct ggggggatct gcgatgaccg taacttcttc
     2521 gaagctggcc ctggcttctg ggtatcacga cacgtctccc gccaaggcct cgcgaacggc
     2581 ccgtccgacc gacgtggttg tggcgtcccc ccccaggtcg ggggttcggg gtgctggctg
     2641 cgacaagacc tggcgaatgg ctgcgcggat tcgtccggca gcgcggggca ggttcatgtg
     2701 gtcgagcatc atggccgccg aaaggatggc ggccagggga ttggcgatac cccggccggc
     2761 gatgtcggga gccgagccat ggaccggctc gaacatgctg ggggccgtcc ggtccggatt
     2821 caagttcgcg gaagcagcca gccccatgcc cccctgaagc acagcaccaa gatcggtgat
     2881 gatgtcgccg aacatgttgg tcgtcacgat cacgtcgtaa tactcggggt tcttcaccat
     2941 ccacatgcag caggcgtcga cgtggttgta gtcggccttt acgtcgggat actcgcgggc
     3001 cacctcctga aacgcccgcc accagagatc gtggccgaag gtgagcacgt tggtcttggc
     3061 cacgagcgtg agcattttgc ccttcggatt attccgccgc cgcgtgaatt cgaaggccca
     3121 gcggatgcag cg
//
LOCUS       DATAQL010000949         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2389359, whole genome shotgun sequence.
ACCESSION   DATAQL010000949 DATAQL010000000
VERSION     DATAQL010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389359"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1021..1686
                     /locus_tag="VHS97_16505"
     CDS             1021..1686
                     /locus_tag="VHS97_16505"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3449857.1"
                     /translation="MMVGDETAETPSWKPPRELAEGLKNGEEKWSRATAQAYYGYNDY
                     DPPPELVAALTDESPAVRFQAVRSLGNYPLNLDSALPTLLSMLEKEDAEARSISEEIL
                     RKAWPTAVVIPNLATALKSENTHVRAMAALLLGRIGPEATSAVPTLVAILIESLESKD
                     EMLVEEAAKALARFGKEASAAIPRLRSLREDKKSAASEAGAVALDAIEGTMKPIQGTS
                     PST"
     gene            complement(1707..2864)
                     /locus_tag="VHS97_16510"
     CDS             complement(1707..2864)
                     /locus_tag="VHS97_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449858.1"
                     /translation="MALDRLKGDTSRSGARLTRPTGQRTDFVNGRRRAFGQIISVMLA
                     VMAITYYVQVASRRQPNAPPSAKVLLGQQEPASDAEAGNAPDTAEPSVVAETPEPPQV
                     TAPPEPQLDRQAVADAEGALDAASRDRARADDRAAQSARQLALATAQAAIDASRAKKL
                     AFLVRDPSTRITQASSRGGFVRGERDKLEKELTTLRNLPRPKSVSILSKSPVARPAAN
                     GESHFELRRNRISFIDLDRLLDLTKADAQVRIRMSDRVAAITNKVGPVGSFSLEYELL
                     KAVPGSVEELLERKSLRYDLRAWELVPEFENRGETYEATKNPISEFTRAVNRINPDRG
                     TITFWIYPDSFTLYRRLRDDLVARGFSVAARPLPDGMSIRGSPMGTQSAAQ"
     gene            complement(2864..3154)
                     /locus_tag="VHS97_16515"
     CDS             complement(2864..3154)
                     /locus_tag="VHS97_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449859.1"
                     /translation="MLLHPGGYRLTTQAMRTQGDSKEGLLVRELKAMVRKRAIVDPMI
                     RPKPKLKFLVESNGSETYWTARRQLLFSLPDWPMSLQVSGTHDPHVFTKETW"
     gene            3309..>3504
                     /locus_tag="VHS97_16520"
     CDS             3309..>3504
                     /locus_tag="VHS97_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449860.1"
                     /translation="MALVVDDELMGIPPVELVDEADELEISGISNAGTAVPDGGADTG
                     TDGGAALESSDPPAGWPAWPS"
BASE COUNT          720 a         1021 c         1070 g          693 t
ORIGIN      
        1 ttgagactat ctcgttgcct cgttgtagcc acagctttcg tcgcgatcgc caactcggga
       61 tgcgacagtt caccgcctcc cgcgccccca gtcgcgagag cagagaccac cgtcacccca
      121 gcaaaaagcg aaagtgtcaa gaagaactgg aagcaaatgc agccgcgggg cttcatgaaa
      181 actgggtgac ctgtgcctga gcatggaatg cgccagagaa gagttaacct ctgagaacaa
      241 agactcacga gtcatcactt tcccttgttt gcaaacactg agggtacatg agccgcggct
      301 aagtagaccc gccgaagtcg tcgcgcagcg taggtcgggc tttctagccc gacgttttgc
      361 tggagggcac aaagtgtcgg gctggaaagc ccgacctaca caaatagttc tgcggactca
      421 cttagtcccc cggcgtgaaa acaaactttc tcggactatg tacgcttcgg ccccatgaat
      481 gatcacactt cggtccggaa tgattcacga acctttcgtc gtctcgggct cagcacgaaa
      541 aatctgacgt tactgatcgc catctcggca ctacttgttt ggtccgggtg gcgaatctgg
      601 gacaatttga gcaaaggcga agacactcgt gtcttcgaag acaccctcgg cagactccaa
      661 tccaccgatg cgtccgagag atggagagcc gcgggaagtc ttcacattgt gaatcagatc
      721 gatcagatcg agccggcgat cgcagctttg gtacgcgccc tcaatgatca agacccgcaa
      781 gttaaagacg cgtcggcacg atcgctcggt tcgctggtcc gccgtcttcg aattcccgcc
      841 ggtagcgatg tcactgccga gccccaattg gttcgcaaga tcacggagca tgcaataact
      901 gctctggccc aatcgttgtc tgattccgct gaatgcgtgc gcgtcgggtc ggcggtcgga
      961 atagggatgc tatcgaccca acccaattcg gcgccgagcg gcggtggaat gcgctctccc
     1021 atgatggtcg gtgatgagac tgcagaaact ccgagttgga aaccgcctcg cgagctggcc
     1081 gagggactga aaaatggcga agaaaaatgg tctcgagcta cggctcaggc gtactacggc
     1141 tacaatgatt acgatcctcc tcccgagctc gttgccgctt tgacagacga gtcccccgcg
     1201 gttcggtttc aagctgtccg gtcgctcgga aattatcctc tcaatctcga ttcagccctt
     1261 cccacgctgc tttcgatgct ggaaaaagag gatgcagagg caaggtcgat ctctgaggaa
     1321 attttaagaa aggcttggcc gaccgcggtc gtgattccga atctggcaac agctttaaag
     1381 agcgaaaata cccacgtgcg tgctatggcg gcgcttctgc taggccgaat cgggccagaa
     1441 gcgacctccg ccgtgccgac gcttgtggcg attctcatag agagtctcga atccaaggac
     1501 gaaatgctcg tggaggaagc cgcgaaagcc cttgcaaggt ttggtaagga agcgtctgcg
     1561 gcgatcccga gacttcgctc tcttagagag gacaaaaagt ccgcggcgag cgaggccggg
     1621 gcggtcgctc tcgacgcgat cgagggaact atgaagccta tacaagggac aagcccaagc
     1681 acctgacgcg gctgatgaga aggtgctcac tgcgccgccg actgcgttcc catggggctg
     1741 ccgcgaatcg acatgccgtc gggtaaagga cgagccgcga cgctgaagcc acgcgctacc
     1801 aggtcgtcgc gcagccgacg ataaagagta aaactgtcgg gataaatcca aaaggtgatc
     1861 gtgccgcgat caggattgat tcggttgaca gcgcgggtga attccgaaat cgggtttttc
     1921 gtcgcctcgt aggtttctcc gcggttctcg aactcgggga ccaattccca ggctcggaga
     1981 tcgtagcgca agctcttgcg ttcgagaagt tcctcgacgc ttccgggaac cgcctttagg
     2041 agttcgtatt cgagcgagaa cgagccgacc gggccgacct tgttggtgat cgcggccacg
     2101 cgatcggaca tcctgatccg cacctgtgcg tcggctttgg tcaggtcgag cagccgatcg
     2161 aggtcgatga agctgattcg gttccgacgc agctcgaaat gcgattcgcc gttggcggca
     2221 ggccgcgcga ctggactctt gctcagaatt gagaccgact tgggtcgcgg aaggttgcgt
     2281 aaagtcgtca actctttctc gagtttgtcg cgttcgccgc ggacaaagcc gccgcgggac
     2341 gacgcctggg tgatccgggt ggatgggtcg cgtaccaaaa aggccagctt ctttgccctc
     2401 gacgcgtcga tggctgcctg ggccgtcgcg agcgccagct gccgggccga ctgggcggcc
     2461 cggtcatcgg cccgcgctcg atcgcgactt gccgcgtcga gcgcgccctc ggcgtcggcg
     2521 accgcctgcc ggtcgagttg cggttccgga ggcgccgtca cctgcggcgg ttcgggcgtt
     2581 tcggccacga cggatggctc ggccgtgtcg ggcgcattgc cagcctccgc gtcgcttgcc
     2641 ggttcctgct gacctaacag gaccttggca gacggtggcg cgttcggttg ccgccgtgag
     2701 gcgacctgga cataatatgt aatcgccatg acggccagca tcacgctgat gatctgtcca
     2761 aaggcgcggc gccgcccatt cacgaaatcc gttctctgcc ccgtgggacg cgttaatcgc
     2821 gcgcccgaac ggctcgtatc gcctttgagt cggtctagtg ccattaccac gtttccttgg
     2881 tgaaaacgtg cggatcgtgc gttccggaca cctgaagaga catgggccaa tcgggaagcg
     2941 aaaagaggag ctggcggcgt gccgtccagt acgtttcgct gccgttggac tcgaccagga
     3001 acttgagctt cggcttgggg cggatcatgg gatcgacgat cgcccgtttc cgaaccatcg
     3061 ctttaagctc gcgtaccagc aagccttctt tgctatctcc ctgcgtgcgc atggcctgcg
     3121 tggtcaggcg atatccgccc ggatgcaaaa gcaagtcgcg ctcacggcaa acaacgacaa
     3181 tctcgaatgg aacctcgatc gagcctcggg gaggctcccg tttgggagca ggtgcaaaaa
     3241 ctcgatcatt gtccgaggag tccgatcccg aactgctatc ttgtccgaaa agactggacg
     3301 aggacgatgt ggcactggtg gtcgacgacg agctcatggg aatacctccc gtagaactcg
     3361 tcgacgaagc cgacgagctg gagataagcg gcatctcgaa cgccggcacg gcagtgcctg
     3421 acggcggcgc cgacacgggg accgacggcg gcgccgcgct tgaaagctcc gatccgccgg
     3481 cgggttggcc ggcttggcct tctt
//
LOCUS       DATAQL010000950         4399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5288634, whole genome shotgun sequence.
ACCESSION   DATAQL010000950 DATAQL010000000
VERSION     DATAQL010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5288634"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(507..1124)
                     /locus_tag="VHS97_16525"
     CDS             complement(507..1124)
                     /locus_tag="VHS97_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449861.1"
                     /translation="MRRIGTVDDQVLAPLVGPVFTGGPPWPSSRQCWRVVRNGPRTIL
                     FSDGLSDPFAGEDGPNTGFGIEVVGETADALNERLELSWLYAVINEVSQQAAAYGVFR
                     RRIDELGLISMGIEALTDQLAALAGPGGHVGLLLGLQPPGWPMEWHLPGGSVKLVTVK
                     LLQPAELAFVLKRGEAGREHLRDLFASDGSNHVSSLSRPSVVGEA"
     gene            complement(1387..2658)
                     /locus_tag="VHS97_16530"
     CDS             complement(1387..2658)
                     /locus_tag="VHS97_16530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3449862.1"
                     /translation="MTNIAFTLLATLLVSPILTKADEPNSALARDVEVFPRGEPAKLG
                     INADALEKLRKRAEETSSAAVVIIKDGQLVADWDFGQKRGPIEAMSATKSIVSLAIGR
                     LVYAGKIKSVDQPVCDFYPEWKQGRKKNITIRHLLNQTSGLQNVPSTGPEIYPSPDFV
                     QLALAAELSDDPGSKFSYNNKAVNLLAGVVKQASGGIRMDQYLGKEIFEPLGIKDFSW
                     TLDRAGNPHGMSGLQIRAIDLAKIGQMMLDRGSWKGKQILSEDWVRKSVEPSQEYYPL
                     YGLLWWLHFQSATVSVTDDFISYFKNHGMTPSSVEKLEELKGKPIDIQTFQRTLLTIV
                     LGDEPINKNLDGLRDGPQLKPVVQGPLRSYEAQGYLGQFLVVVPRDRIVAVRMRRGPG
                     GDDTALELKRSFVDFADMVGKLADNPAGTKG"
     gene            complement(3249..>4399)
                     /locus_tag="VHS97_16535"
     CDS             complement(3249..>4399)
                     /locus_tag="VHS97_16535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3449863.1"
                     /translation="ATPTFASLCLAVSAVMVASGVPPFTRDAKLGWAWGANFGPSVAL
                     DGETWRLLTCMFLHYGLLHLGFNLWFLLKFGPPVERLFGTGGFAALYLLSGLGGSIAS
                     LWYHPMAVSAGASGSLFGVAGALLGYLVARRDVPPRAILQLFRPGLLIYLGVDSLLGL
                     MVPQVDGAGHVGGLATGFACGLALGRPWPPPPAAYAPRRRLAVVGLLATGLVVLLVAL
                     MPGIRARLKREPEIIAYYDSQEAQKAYKAFVRAIERAADRYDAIGEELDEWTSRLDRR
                     AMPVGPAIEALGRLTSRADANARVLCTLSVKEPELRVALDRLASAQSHQHQALDALRR
                     YLETGDRRLLAGARGYEAHLRSGGDDIRSFNESCARYLKAHDLTSNDP"
BASE COUNT          838 a         1436 c         1345 g          780 t
ORIGIN      
        1 aggtccagta cccgcatgcc gggccgcagg tcgagcgcct cagtgagcca ttcggtcagc
       61 cacagggggt tggctccgcc gcttaagcca gcgagtaccc actcggggtg gtagcccgag
      121 gcgcggggga accggtcagc gttcaaccgc tcgtccacgg ccatgcgccc ctcctggccg
      181 aacgatgatt cgacagaatg ccatccgtct aaatcacctc ggctttctgg ctaagaccgc
      241 tcacaagatg cagcgcagcg agccaccgcg agtcgcaaga ttcaacccgt gactggccca
      301 aggtatccaa ccagttgggc cgtcgtcaac aaactaccgg gaaagtacga ttgatgtccg
      361 aatccttgtc ttccgccccc agatctcaac ggcgcggcaa ctccagggac gctccagatc
      421 agcggcccgc cgacgggcgg gtcggctgca tcgcctcgtt aggcaccgag cagataacgc
      481 cagaaccgcc gtcatggctt gccctcttaa gcctctccaa cgaccgaggg ccgcgatagc
      541 gaggaaacgt gattgctccc gtcggacgcg aacagatccc gtaagtgctc ccttcccgcc
      601 tctccccgct tcaagacgaa ggccagttct gcgggctgca gcaatttgac cgtgacgagc
      661 ttgaccgagc cgccgggcag atgccactcc atcggccagc ccggaggctg cagccccaac
      721 agcaatccga cgtgccctcc cggacctgct agggcggcca actgatcggt cagagcctcg
      781 atccccatgg agatcagccc cagctcgtcg atcctgcgac gaaacacgcc gtaagcggcg
      841 gcctgttgag agacctcgtt gatgaccgca tagagccagc tcagctccag tcgctcattt
      901 agtgcgtcgg ccgtctcgcc cacgacttcg atcccgaaac cggtgttcgg cccgtcctcg
      961 cccgcgaagg gatcagagag tccatcgctg aagaggatgg tccgtggccc gttgcgcacg
     1021 accctccagc actggcgaga cgagggccaa ggggggccgc ccgtgaacac cggtccgacg
     1081 aggggcgcga gcacctgatc atcgaccgtt ccgatccgcc gcaactaggc ttcgcgggct
     1141 tggaatagac actgcaagga gtctccatgc atctcgcacc atagctggcg agtaaccggc
     1201 gaggtggtga agggctggaa gaacgcattc attgtgcacc tcgccgcgaa gccgaacttg
     1261 tgattctctc acgaaaacgc actgatgatg atgggccaaa gtcggcctgt caatcagcga
     1321 acttcctaac gactcaatcg ccgggtctca ttaacggtct gggagatggc agcgctgtcc
     1381 ccatgctcac cctttcgttc ccgccggatt atcggcgagc ttgcccacca tgtcggcaaa
     1441 atcaacgaag ctgcgtttta attcgagcgc agtatcgtcc ccgccgggcc cacggcgcat
     1501 gcgaacggcc acgatccgat cgcgcgggac gacgaccagg aattggccga gataaccctg
     1561 cgcctcatag ctgcgcagcg gtccttggac gaccggcttc agttgtggtc cgtctctcaa
     1621 cccgtccagg ttcttgttga tcggctcatc gcccaggacg atcgtgagca gggtcctctg
     1681 gaaagtctga atgtcgattg gctttccctt cagctcttcg agcttctcga ccgatgaggg
     1741 cgtcatcccg tgatttttga aataactgat aaaatcatca gtaacagaaa ccgttgcgga
     1801 ttggaagtgc agccaccaga gaagaccgta cagagggtag tactcctggc tcggctcgac
     1861 cgacttgcgg acccagtctt cactcaggat ttgtttgccc ttccacgatc ctcggtcgag
     1921 catcatctga ccgatcttcg ccaggtcgat ggcacggatt tgcaagcccg acattccatg
     1981 gggattgccg gctcgatcga gggtccagct aaagtccttg atgccgagcg gctcgaagat
     2041 ctccttgccg aggtactggt ccatgcgtat tcctccggat gcctgcttca caactcctgc
     2101 caacagattc acggccttgt tgttgtagga gaatttcgag cctggatcgt cgctcaactc
     2161 cgctgcgagc gcaagctgca cgaagtcggg actcggatag atctccggcc ctgtggacgg
     2221 cacgttctga aggccgctgg tttgattcag caaatggcga atcgtgatat ttttcttgcg
     2281 tccttgcttc cactccgggt agaagtcgca gaccggctga tcgaccgatt tgatcttgcc
     2341 ggcgtagact agtcgcccga tcgccaggct cacgatcgac ttggtggccg acatcgcctc
     2401 gatcggcccg cgtttctggc caaagtccca atcagcgaca agctggccgt ccttgatgat
     2461 cacgactgcg gccgaactcg tctcctccgc gcgcttccga agcttttcca gcgcgtcggc
     2521 gttgatgccc aacttcgccg gctcgcctcg aggaaacacc tcgacgtcgc gcgccagagc
     2581 cgagttcggc tcgtcggctt tggtcaagat tggagaaacg agcagcgtcg caagcagcgt
     2641 gaatgcgata tttgtcatgg ttagccttct ttgagttcca gaggagaggc acatcgacgg
     2701 ctcgcgcaat cgaataacgc agcgtgcctc aacctttggc ttcgagtatt gcacggcctt
     2761 tccctaattt acctttcgtt cgccgccgtg agctcgagtt ttcggcagcg ctactgagct
     2821 gtacgcgcga accttgtggc cctcgggcgt aacgccctga aacggaaacc gaccggcgct
     2881 cggcccggat cagccagcag cacgggggga cgcctcgtcc tgggacagac caaagcgagc
     2941 accagccgcc gcagctcggc attgacccag cgtgcaagaa agcgcttgtc atcgtagctt
     3001 gccgccgcct gccgtcgaca tgtttgttta acgcttccga tgaacaactt catcaggctc
     3061 ggccttggag cggtcttgcg cgcttcggtc cgacgacacc gaagcatcga gcaagcgatc
     3121 agcgcgcttc ggtcgatcag accgacgcgc gcaaggatac cggccatgct cacgccgtag
     3181 taggcgtcga gtgaggaacc actcgcccaa ttgtccgggg ctcgatcctc cgactcgggc
     3241 ggccggattc agggatcgtt gctcgtcagg tcgtgggcct tcagatacct cgcgcacgat
     3301 tcgttgaagg accggatgtc gtcgccgccc gaccggagat gggcctcgta accgcgcgct
     3361 cccgcgagca ggcgccgatc tcccgtctcc aggtagcgcc gcaacgcgtc cagggcctgg
     3421 tgttggtggg actgggccga ggccaggcga tcgagcgcga cccggagttc gggctccttg
     3481 acggagaggg tgcacaggac cctggcattc gcgtcggcgc gggaggtcag cctaccgagg
     3541 gcttcgatcg ccggaccgac gggcatggcc cgccgatcga gcctggaggt ccattcgtcg
     3601 agttcctcgc cgatcgcatc gtagcgatct gcggcgcgct cgatcgcgcg cacgaaggcc
     3661 ttgtaggcct tctgggcctc ctggctgtcg tagtaggcga tgatctccgg ttctcgcttc
     3721 agccgcgccc ggatgccggg catgagggcg accaagagca cgacaaggcc ggtggcgagc
     3781 aatccgacca ccgccagccg gcgccgcggg gcataggccg cgggcggcgg cggccagggg
     3841 cggccgagcg ccaggccgca ggcgaacccg gtcgcgaggc cgcccacgtg gcccgcgccg
     3901 tcgacctgcg gcaccatcag gcccaggagc gaatccactc cgaggtatat caacaggccc
     3961 ggccggaaca attgcaggat cgcccgcggg gggacgtcgc ggcgggcgac caggtagccc
     4021 agcagggcgc ccgcgacccc gaacagagac cccgacgcgc ccgccgacac ggccatcggg
     4081 tgataccaga ggctcgcgat cgagccgccc aggcccgaca ggaggtagag cgccgcgaag
     4141 cccccggtgc cgaagagccg ttcgaccggc ggcccgaact tcaggaggaa ccagaggttg
     4201 aagccgaggt gtagcaatcc gtagtggagg aacatgcagg tcaggagcct ccaggtctcc
     4261 ccgtccaggg ccaccgatgg gccgaagttc gctccccagg cccagcccag cttcgcatcg
     4321 cgggtgaacg gagggacgcc cgaggccacc atcaccgcgg agaccgccag gcagagcgac
     4381 gcgaacgtcg gagtggcga
//
LOCUS       DATAQL010000951         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1472061, whole genome shotgun sequence.
ACCESSION   DATAQL010000951 DATAQL010000000
VERSION     DATAQL010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472061"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VHS97_16540"
     CDS             <1..700
                     /locus_tag="VHS97_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3449864.1"
                     /translation="PAQYDGNQPICVMVFQDGSGYTSFVPTVFDNLIAKGEMPATAAV
                     FINPGNGPGEEGRGQRSLEYDTLSDRYSRFLLEEILPDVEKTVKLRHDPESRAIAGIS
                     SGGICAWTVAWERPDEFRRVLSWVGSFTNIASGKSRHDGGHNYEALIRKSPKKPIRVF
                     LQDGANDLDNANGNWPLANQQMAKALEFAHYDYQFVFGNGFHSNRHGRAILPDSLRWL
                     WRDYKPGDETKSSN"
     gene            complement(824..2035)
                     /locus_tag="VHS97_16545"
     CDS             complement(824..2035)
                     /locus_tag="VHS97_16545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX3449865.1"
                     /translation="MRSISRRLALMIVTLFLAVGAARAGDAYVQTNLVSNTPGLAPVT
                     DPNLQGAWGQSFTTNSPFWISNQASGTSTLYSVSPTNPTSASIVPLVVPITNQGNPPA
                     AANPSTNGPTGQVAVGAPGITSQTTDFLVNGTQAHFIFANKDGSISGWNGTNPAVIQT
                     TVSGASYTGLASGNLPGGGAAQLYAADHNSPSGSIDVFNSKFQMTGSFNDPNFSKLPS
                     GFNAFNVQNLNVNGTQTLFVTYANQATTGGIVDEFTTNGTFIKTLISDPTGAHLAAPW
                     GMAIAPASFGQFGGDLLVANDNANSAGVTGINAYNLTTGAFDGTLTLANGQPFSEAKL
                     WSLSFGSGQGGGGSSDVLYFTAGLNGNSGGLFGAISAVSVPEPSSAALGLIAASVMFG
                     GFTWKKRRRRA"
     gene            complement(2501..3784)
                     /locus_tag="VHS97_16550"
     CDS             complement(2501..3784)
                     /locus_tag="VHS97_16550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3449866.1"
                     /translation="MNELMTGESLDAGRNGPLEAAAPPIESGASCLRATSWWPVAIGA
                     LTATALAGALVAGTLPRLHAEKELKAAAEEVSTSLPRVTVVTAQTATAATERVLPGNC
                     HPLLEAAIYARTTGYLKSRRVDIGDRVKEGDLLAEIATPEVDAQLEQARATLLLTQAN
                     LLRDQATEELAKIDLNRSRHLLARQAVPQQEFDSYAAQAKIAGANVKATESTLKVYEA
                     NIQRLETLQSFQKVTAAFSGVITARNIDPGDLVSADSPTSSREMFHLVQMDILRVFVN
                     VPQVFSTDVKVGQKAIVYRREDPKRTFAGTITRTADALDTNTRTLLTEVQVPNEGAAL
                     RPGMYLQVKFIFERKVATVVIPAAALATRSDGPRLAVMDDQHCVHYRQVQLGRDFGTN
                     TEVLSGVEAGQTVVVHPGDDLPEGTQVEPLDVPTK"
     gene            complement(3781..>4696)
                     /locus_tag="VHS97_16555"
     CDS             complement(3781..>4696)
                     /locus_tag="VHS97_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449867.1"
                     /translation="YQAGPSSSSSRGAQGPGLAPPQLLSNLAALSRTVSESVVSHYNV
                     QPVFEVYANVQGRDLGGVSADVNRVVDLVRPDLPRGSSIAIRGQVESMNSSFSGLAFG
                     LVFAVLLVYFLIVVNFQSWLDPFIILTALPGALAGILWMLFVTQTTVSVPALMGAIMC
                     IGVATANSILMITFANDQRRLGLDAHDAALAAGSTRLRPVLMTALAMIVGMLPMSLGL
                     GEGGEQNAPLGRAVIGGLTVATFFTLVFVPVLYTVLRRKPPAHDLADDAMVEATMDTA
                     FETTTHHSPNNRLLVATSEPLASQEGRS"
BASE COUNT          924 a         1444 c         1459 g          869 t
ORIGIN      
        1 cccggctcag tacgacggga accaacccat ctgtgtcatg gtcttccagg acggatccgg
       61 ctacacctcc tttgtgccca cggtcttcga caacctgatc gccaaaggcg aaatgcccgc
      121 gaccgccgcg gtgttcatca accccggaaa cggcccgggc gaagagggcc gcggccagcg
      181 aagcctcgaa tacgacacgt tgtccgaccg ctactcccga ttcctgctcg aagaaattct
      241 ccccgacgta gaaaagacgg tgaagcttcg ccacgacccc gagagccgag cgatcgccgg
      301 catcagcagc ggcggtatct gtgcctggac ggtcgcctgg gaacggccgg acgaatttcg
      361 cagggtgctc tcgtgggtcg gtagcttcac caatatcgcc agcggcaaga gccggcacga
      421 cggaggccat aactacgagg ccttgatccg taagagcccc aagaaaccaa tccgcgtctt
      481 cctccaagat ggcgccaacg acctcgacaa cgctaacggc aactggcccc tggccaacca
      541 gcagatggcc aaggcccttg agttcgccca ctacgattat caattcgtct ttggcaacgg
      601 ctttcatagc aatcgccacg gccgcgcgat cttgcccgac tccctccgat ggctctggcg
      661 cgattacaag cccggcgatg agaccaaatc cagcaattaa gggaggatcg cgcaatcagc
      721 gaccctcccc atacttacga aacgcccagg gcttgaagag attcttccgt cttctcgaca
      781 cgagggaagt ggaagacgga agagtctcgc ttcatgaagg aaatcaagcg cggcgacgac
      841 gcttcttcca ggtaaatcct ccgaacatga ccgacgccgc gatcaacccc agggcggccg
      901 agctcggctc gggaacggac accgccgaga tcgcgccgaa gaggccgccc gagttgccat
      961 tcagcccggc ggtgaagtag aggacgtccg aggaaccacc gccgccctga ccgctaccga
     1021 agctcaggga ccaaagttta gcttcggaaa aaggctgacc attggcgagc gtcaacgtgc
     1081 catcaaatgc accggtcgtg agattgtagg cattgatccc tgtaacgccc gcggagtttg
     1141 cgttgtcgtt ggcgacaagc aggtcgccgc caaactggcc aaaactcgcg ggcgcgatgg
     1201 ccatccccca gggagcggcc aggtgggccc ctgtcgggtc gctgatcagc gtcttgatga
     1261 aggtgccgtt cgtggtgaat tcatccacga tgccgcccgt ggtcgcctgg ttcgcgtacg
     1321 tgacaaagag ggtctgtgtc ccgttgacgt taaggttctg gacgttgaac gcattgaagc
     1381 cgctgggaag tttcgagaaa ttcgggtcat tgaaacttcc cgtcatttga aacttgctat
     1441 tgaagacgtc gatgctgcca ctggggctgt tgtgatcggc ggcatataac tgggcggcac
     1501 ccccgcccgg caggttcccg ctcgccagcc cggtgtaaga ggcaccagag acggtcgtct
     1561 ggatcaccgc ggggttagta ccgttccacc ccgagatcga gccgtcctta ttggcgaaaa
     1621 tgaagtgggc ctgggtaccg ttgacaagga agtccgtcgt ctgcgatgtg atccccggtg
     1681 cgccgacggc cacctggccc gtcgggccat tggtgctggg gttcgcggca gcgggagggt
     1741 tcccctggtt cgtgattgga acgaccagcg gcacaattga ggcgctcgtg ggattcgtgg
     1801 gactcacgct gtagagcgtc gaagtccccg acgcctggtt ggagatccag aatgggctgt
     1861 tcgttgtgaa ggactgcccc caggcaccct gcaagttcgg gtcggtaacc ggtgccagcc
     1921 ccggggtatt cgagacaagg ttcgtttgga cataagcatc cccagcccgg gcggcgccca
     1981 cagcgaggaa gagggtaacg atcatcaagg caagtcgacg actgatcgag cgcatgtact
     2041 tggttctcct tgagcgccgg gcgcgcaccg gcgcccgagc cgactgtttt cggttgaaga
     2101 ctctgtgaag gctcgttcct acgcaactcg actccgatcc tcatcctccg gaggagccgg
     2161 tcaatctctg atagacaatg agtctcgatc gggcgagcgg gttacaggtt ttcaaaaaca
     2221 atctggcatc tcctgatgca agggacggtg aactcgcccg cgtccggaag ctccaacaca
     2281 cgagtgattg cgcggttgga cggcggtcta agcagcgtgg gaacagggca cggagtgtcg
     2341 cattcccggc ggctagctgc ctcttaatcg gaccgggcta tcctggtacg gctgtcgtag
     2401 gttgccgcca acgatcgcca ttccgcttgc gatcgcgccg cgtcgtcgcg aggccgcgtc
     2461 gtcgatccgg cgcgcgattc caccgtggga gcggcgccga ctacttcgtc ggcacgtcga
     2521 gcggttccac ctgggtccct tcgggtagat cgtcgcccgg atggacgacg acggtctggc
     2581 cggcttccac tcctgacaga acctccgtat tcgtaccgaa atcgcgacca agttgcactt
     2641 gtcggtaatg cacacaatgc tggtcgtcca tgacggccaa ccgcgggcca tcggaccggg
     2701 tcgccagcgc cgcggccggg atcacgactg tcgccacctt tcgctcgaag atgaatttga
     2761 cttgcaagta catgcccggc cgcagtgcag ccccttcatt gggcacctgc acctcggtca
     2821 gcagcgtgcg agtgttcgta tccagcgcgt ctgccgttcg ggtgatcgtg ccggcgaatg
     2881 tccgtttggg gtcctcgcga cggtagacga tggctttctg gccaaccttc acatccgtcg
     2941 aaaacacctg gggaacgttc acaaacacgc gcagtatgtc catttgcacg aggtgaaaca
     3001 tttcgcggga tgatgtcggg ctatcagcgg agacgagatc acccggatcg atatttcggg
     3061 cggtgatgac gcccgagaac gctgcggtca ccttctggaa ggattgaagg gtctccagcc
     3121 gctgaatgtt ggcctcatag accttgagtg tcgactcggt ggccttgacg ttcgcgccgg
     3181 cgattttggc ctgcgccgcg tacgaatcga attcctgctg aggcaccgcc tgacgtgcaa
     3241 gcaaatggcg cgaccggttc agatcgatct tggcaagctc ttccgtggcc tgatcgcgga
     3301 gcagattggc ctgagtgagg agcagggtcg cgcgcgcttg ctcgagctgg gcgtcgacct
     3361 cgggcgtggc gatctcagcg agcaggtccc cctccttgac gcggtcgccg atatcgaccc
     3421 gccggctctt caaatagccg gtggtccgcg cgtagatggc cgcttcgagc aagggatggc
     3481 aattgcccgg caagacacgc tcggtcgcgg cagtcgccgt ttgggccgtc actaccgtaa
     3541 cccgcggtag cgacgttgaa acctcctctg cggccgcttt gagctctttt tccgcatgga
     3601 ggcgcggcaa tgtccctgcc accagggccc ccgccagggc agtggccgtc agcgcgccaa
     3661 tcgcgacagg ccaccacgag gtcgccctga gacacgaagc gcctgattcg atcggcggcg
     3721 cggccgcctc caatgggccg ttccgtcctg cgtccaagct ttcaccggtc atcagttcgt
     3781 tcatgatcgt ccttcctggg aagccagagg ttcgctcgtc gctacgagca gccgattgtt
     3841 cggggaatga tgcgtcgtcg tttcaaaagc ggtgtccatc gtcgcttcga ccatcgcgtc
     3901 gtcggccaga tcgtgggcag gcggtttacg ccgcaagacc gtgtacaaga cgggtacgaa
     3961 gactagagtg aaaaaggtgg cgacggtgag accgccgatg acggcacgcc ctagcggagc
     4021 gttttgctcg ccaccttcac ccaggcccag cgacatgggc agcatgccga cgatcatcgc
     4081 cagggccgtc atcaaaacgg ggcgcagccg cgtcgaaccc gcggccaggg ctgcgtcatg
     4141 agcgtcgagg ccaagacggc gctgatcatt ggcaaaggtg atcatcagga tgctgttggc
     4201 cgtcgccaca ccgatgcaca tgatcgcgcc catcagtgcc ggcacactca cggtcgtctg
     4261 tgtcacgaac agcatccaga ggatgccggc taatgcaccg ggcagagcgg tcaggatgat
     4321 gaacgggtcc agccacgact ggaagttgac cacgatcaag aagtagacca agagcaccgc
     4381 gaatacgagg ccgaaggcaa gtccagagaa cgagctgttc atgctctcga cctgtccgcg
     4441 gatcgcgatt gaactgccgc gcggcaagtc tggtcgcacc aagtcgacga cgcgattgac
     4501 gtcggccgag acgcctccca ggtcgcggcc ctggacgttg gcgtaaactt caaaaacagg
     4561 ctgaacgttg tagtggctca cgaccgattc cgaaacggtc cgactcaggg ctgcgaggtt
     4621 gctcagaagc tgcgggggcg ccaggccggg cccctgcgcg cctcggctcg acgacgaact
     4681 tggcccggcc tggtag
//
LOCUS       DATAQL010000952         2972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3150457, whole genome shotgun sequence.
ACCESSION   DATAQL010000952 DATAQL010000000
VERSION     DATAQL010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3150457"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VHS97_16560"
     CDS             complement(<1..359)
                     /locus_tag="VHS97_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449868.1"
                     /translation="MSAIAHSKRVSMLAGGGHAEPIGLLAGSGRFPILFAEAARRQGL
                     PVACMGIKYEASEELRGLCDSFQWVGVAKLGGMIRGFRRQGARRIVMAGKVTKNVIYT
                     PWRVLQLWPDARTVQWW"
     gene            complement(405..1736)
                     /gene="lpxD"
                     /locus_tag="VHS97_16565"
     CDS             complement(405..1736)
                     /gene="lpxD"
                     /locus_tag="VHS97_16565"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX3449869.1"
                     /translation="MVATVEQLAELVRGRLVGDGSVSIQSARPVAEAGPGDITFIEND
                     RFAKLLRSSPASAAIVGPHFSAGRNEVGQSLAVIEVDDPMSAFLAVRTHLIGEQTKQW
                     TGIHPQAYVAPTAQLGKDVAIYPFAYVGEGAVIGDGTTIRPGAVISEGCTLGRDCIIH
                     PNAVLYKNVILGDRVEIHGGSVLGGDGFGYRQTDGRHVKIPHSGGLTLDHDVEVGSNC
                     TIDRATFETTRIGEGTKIDNLVMIGHNNQIGRHNLLCGQVGIAGGCKTGDYVVMAGQA
                     GIKDKTQIGDRVKVGAQAGVHRNIPDGQTVLGSPAVPVREQRRIFQMIARLPEMHRQL
                     RELTAQIEALTALAAQGSGALDGLANDHAAISASGTDLLAMNSIGHGLEARATRAEGG
                     TGILPVNSIGHGLEARATRAEGGTGILPMNSIGHRLEACATRAEDSDNESE"
     gene            complement(2026..2742)
                     /locus_tag="VHS97_16570"
     CDS             complement(2026..2742)
                     /locus_tag="VHS97_16570"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX3449870.1"
                     /translation="MSRPTTWNGIRGIVFDAVGTLIEPVPSVAEAYTAAAGRQGVVLD
                     PREVRARFHVHFQNDGVHAQRGLLSTDEETERRRWRMIVTGVLPEVAEPDRAFDELWD
                     HFSQPMSWRCFSDVAVALDALNEQGISVCVGSNFDGRLRRVVNGLAELRGRAGSLVIS
                     SEVGYRKPHPSFFRAACAHLGLPPEAVLCVGDDLENDVRGAIRAGLSGLLLERGVHKP
                     DSLPHVPNLTALIRSKFGES"
BASE COUNT          577 a          949 c          837 g          609 t
ORIGIN      
        1 taccaccatt gaactgtgcg agcgtcgggc cagagctgaa gcactcgcca cggggtgtag
       61 atcacgtttt tcgtgacctt tcccgccatc acgatccgtc gggccccctg gcgacgaaag
      121 ccccggatca tcccccctag cttggcgact ccgacccact gaaacgagtc gcagagaccg
      181 cgcagctctt cggaagcttc gtacttgatc cccatgcatg ccaccggcag gccctgccgc
      241 cgagctgcct cggcaaacaa gatcgggaat cggccgctgc cggccaagag gccgatcggc
      301 tcggcgtgcc cgccgccagc cagcatcgag acacgctttg aatgggctat cgcgctcacg
      361 cgaaatcgct cccggaaatg tcggtttggg acacgaacag ccagttactc tgactcgttg
      421 tcagaatctt cggcccttgt ggcacaggct tccagcctgt gtccgatcga attcatgggc
      481 aagatgcccg tgccaccttc ggcccgtgtg gcacgggctt ccagcccgtg gccgatggaa
      541 ttcacgggca agatgcccgt gccaccttcg gcccttgtgg cccgtgcttc cagcccgtgt
      601 ccgatcgaat tcatggccaa aaggtccgtg ccacttgcgg aaatcgcggc gtggtcgttc
      661 gccagaccat ccagtgcgcc ggacccttga gcggccagcg cagtcagtgc ctcgatctgg
      721 gcagtgagct ctctgagctg ccggtgcatc tcgggcagcc gggcgatcat ctgaaaaatc
      781 cgccgctgct cccgcactgg aactgccggc gagcccagca ccgtttgccc atcagggatg
      841 ttgcggtgta cgcccgcctg ggctccgacc tttacccgat cgccgatctg cgtcttgtct
      901 ttgatgccgg cttggccggc catcacgacg taatcgccag tcttgcaccc accggcaatg
      961 ccgacttgcc cgcacaacaa gttgtggcgg ccgatctggt tgttatggcc gatcatcacc
     1021 aggttgtcga tcttggttcc ctcgccgatc cgggtagttt caaaggttgc acggtcgatc
     1081 gtgcagttgg aacccacctc gacatcgtgg tcgagggtta gtccgccgga gtgcgggatt
     1141 ttcacgtgcc ggccgtcggt ctggcggtag ccgaacccat caccccccag cacgctgccg
     1201 ccgtgaatct cgacccgatc gccgagaatc acatttttat aaaggactgc attgggatga
     1261 atgatgcagt cgcgccctag cgtacagccc tcgctgatca cggcaccggg acggatcgtc
     1321 gtgccgtcgc cgatcaccgc gccctcgccc acgtacgcaa acggataaat cgcgacatcc
     1381 ttgcctagct gcgctgtcgg ggccacatag gcctgaggat gaattcccgt ccattgcttc
     1441 gtttgttcgc cgatcaagtg agtacgtacg gccagaaagg ccgacatcgg gtcatcgact
     1501 tcgatgacgg ccaagctctg tcccacttca ttgcgccccg cgctgaagtg gggtcccacg
     1561 attgccgccg acgcaggcga gcttctcaag agcttggcga accggtcgtt ttcgatgaag
     1621 gtgatatcgc ccggtcccgc ttcggcgacc ggtcgtgccg actggatcga gacgctaccg
     1681 tcgccaacca agcgaccacg tacgagttcc gcaagttgtt ccaccgttgc gaccacgggc
     1741 gttttccttt cttccttgaa aggagaagac gattaccaat gcgtcgcggc agagcgggtc
     1801 cgattctttg gcccgggcgt gcgggccaga atcgattcga ttcgacgtgg ttacgggacg
     1861 cgcacaatca cgatagcctt ggtggccaag cgaatgctaa tccatgtcgt cttgcggggc
     1921 aagtcgagtc gtccggaccc ggccgacctc gcttttccgc tccttagacg tccgacgcga
     1981 aaacgtttcc tattctggtc gatcgtcccg agatcgtcgc ggacttcacg attcaccaaa
     2041 cttcgaccga atgagtgccg tcaggttcgg cacatgcggt agagaatccg gcttgtgaac
     2101 acctcgttcg aggagcaggc ccgacagacc ggctcggatt gcgccacgca cgtcgttttc
     2161 caggtcatca cccacgcaca ggacggcttc tggtggcaaa cccaagtggg cacacgccgc
     2221 ccgaaagaac gatggatgtg gtttgcgata gccgacttcc gacgaaatca ccagagatcc
     2281 ggcgcggcct ctcagctccg caagcccgtt tacaacacgt ctgagccggc cgtcgaagtt
     2341 cgagccgacg cagacggaaa taccctgttc gttcagggca tccagcgcga ctgcgacgtc
     2401 cgaaaaacag cgccacgaca tgggctgact aaagtgatcc cataattcgt cgaaggcccg
     2461 gtccggctcg gcgacctccg gcaagactcc cgtcacaatc atccgccagc gccgccgttc
     2521 ggtctcctcg tcggtcgaca gtaatcctcg ctgtgcgtgc actccatcat tctggaaatg
     2581 cacatgaaat cgtgcacgca cttccctggg atcgaggacc accccctggc gccccgcagc
     2641 cgcggtatac gcctccgcaa cggatggcac tggctcgatc aacgtgccaa cggcatcaaa
     2701 gacaattccc cgtataccat tccaggttgt tggtctactc atcagtgtgt tttcagcgga
     2761 taacaattgc gaatgcgcga gtttcatagt gctggagtca ttcaaagttt gagaagttca
     2821 gcaaaaacac aaggataggt agtttacact ggcctttttg atcaggaaag tcggtgaggg
     2881 ttcagttgag ccgattcatc agttcaatgt tgacgctgtt ttgtccgtac caagccggcc
     2941 gcaacgtcga tgtcgaggct ggcaacctgc cc
//
LOCUS       DATAQL010000953         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1319114, whole genome shotgun sequence.
ACCESSION   DATAQL010000953 DATAQL010000000
VERSION     DATAQL010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319114"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VHS97_16575"
     CDS             <1..658
                     /locus_tag="VHS97_16575"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEX3449871.1"
                     /translation="RLARASIDDVFIGVGRGEIFSTDVVRAVFPSFKDERRPGPAVPQ
                     NESGWFGLPKAADLVFRVPGEPDAGGALPLRGIDLDTPVKFAPKGGAVPGERIVGVRE
                     EDGIVIYPIHSPALTEFDDRPELWLDVRWDLENAPTALYSTQIVVTLLNEPGALGAIA
                     TTIGEYGANIDSVATSPSGADFRRFTIDLQVRDIQHLNAVLSQLRDKSVVHNVERLSG
                     "
     gene            667..1089
                     /locus_tag="VHS97_16580"
     CDS             667..1089
                     /locus_tag="VHS97_16580"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX3449872.1"
                     /translation="MPLYFAYGSNMDVAAMARRCPRSKAVGLARLERHRLVVMPQGWL
                     TAVRSPSSAVHGVLWDLALSDIPALDRHESLSQGLYTKETQPVIAERGPKRAIVYFGA
                     NAGPGVLRPAYIAEVLAAARSWALPAEGVAALERLAQR"
     gene            1333..2409
                     /locus_tag="VHS97_16585"
     CDS             1333..2409
                     /locus_tag="VHS97_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX3449873.1"
                     /translation="MRKMMKAAIFVEKGRIVLDDKPIPDIGPQDALIRVTTTTICGTD
                     VHILKGEYPVAKGLTIGHEPVGVIEKLGSGVVGFKEGQRVIAGAITPSGWSHACLCGC
                     GSQDGAGTKHGWQAIGGWKFGNTIDGCQAEFVCVPDAMANLAPVDDAVSDEQVLMCPD
                     IMSTGFAGAQTGEIKIGDTVAVFAQGPIGLCATAGAKLLGATTVIGVDTVPARLAMSK
                     RMGADHVVDFRKEDPVEAIMRLTDGRGVDVAIEALGTQATFEAALRVIRPGGVLSSLG
                     VYSSDLKIPLDAFAAGLGDKSIRTSLCPGGKERMRRMLAVISSGRVDLGGLVTHRFKL
                     DQIEEAYDLFANQRDGVLKVAVSV"
     gene            complement(2651..3283)
                     /locus_tag="VHS97_16590"
     CDS             complement(2651..3283)
                     /locus_tag="VHS97_16590"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEX3449874.1"
                     /translation="MLSRLFRFVAAAVVFGFAWNTFVEASEQGLPQATRAPVGGEASI
                     PYGWVDFCNRHSEECTPGKLKPMDVRLTRQSWKVLNEINNRVNNAIEPISNFDHWGTT
                     LDHWDYPVDGKGDCKIYALFKRKLLIDRGFPRQALLMTIVRDLNGEGHAVLTVKTDRG
                     DFVLDNLSDEIRPWTATGYQFYKRQAQDDPNVWLSLGGATGAEPGTAASN"
     gene            3567..>4693
                     /locus_tag="VHS97_16595"
     CDS             3567..>4693
                     /locus_tag="VHS97_16595"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX3449875.1"
                     /translation="MSTEATSAEPQSIGRLGLMGLAALGVVFGDIGTSPLYTLKTVLD
                     YTGRHPDPAATLGALSLILWTLFLITTVKYVNIAMRIDNDGEGGIIALMTLLGVKQHQ
                     RPLIIAVGLFGAALIYGDGAITPAISVLSALEGVEQIAPSLQTYVLPAATVVLLALFA
                     VQPFGTAKIGRAFGPIMLIWFASIAALGVYGVLQHPSVFAAVDPRYGLDYLSRGGLEG
                     FLVLGGVFLCVTGSEALYADMGHFGPKPIRASWILIVFPALILNYAGQAAIVLEGAPT
                     QGNIFYRLCPGPLLTPLIVLATIATVIASQSIITGAFSMTRQAMQLGWLPRMNITQTS
                     EEGYGQIYVGVVNWLLMIVTLGLTIGFGKSDNLAAAYGIAVS"
BASE COUNT          833 a         1454 c         1495 g          911 t
ORIGIN      
        1 gcgtctggcg cgcgcctcga tcgacgatgt cttcatcggc gtggggcgcg gcgagatctt
       61 ctccacggac gttgtgcggg ctgtgtttcc gagcttcaag gacgagcgca ggccggggcc
      121 ggccgttccg cagaacgaga gcgggtggtt cggattgccg aaagcggccg atctcgtctt
      181 ccgtgtgccg ggcgagccgg acgcgggcgg ggcgctgccg ctgcgcggca tcgacctcga
      241 tacgccggtc aagtttgcgc ccaagggcgg agcggtgccg ggtgagcgca tcgtcggcgt
      301 tcgcgaggaa gacggcatcg tcatctaccc gatccattcg cctgcgctca ccgaattcga
      361 cgatcggccg gagctctggc tcgacgtgcg ttgggacctg gagaacgcgc cgacggcgct
      421 ctattcgact caaatcgtcg tcacgctctt gaacgagccc ggcgcacttg gcgcgatcgc
      481 cacgaccatc ggcgagtatg gcgcaaatat cgactccgtc gcgacctcgc cgagcggcgc
      541 cgacttccgg cgcttcacca tcgatcttca ggtgcgcgac atccagcacc tcaacgcggt
      601 cctgtcgcaa ctcagggaca agagcgtggt ccacaatgtc gagcgcctga gcggttagac
      661 aagtcgatgc cgctctattt cgcctacggc tccaacatgg acgtcgccgc catggctcgc
      721 cgctgcccgc gttcgaaagc ggttgggctt gcgcggctcg agcggcatcg cctggtcgtc
      781 atgccccagg gctggctgac cgccgttcgc agtccgtcct ctgcagttca tggcgtgctg
      841 tgggacctcg ctctgtccga catccccgcg ctcgaccggc acgagtccct ttcgcagggc
      901 ctctacacca aggaaacgca gccggtcatc gccgagcgag gaccgaaacg ggcgatcgtc
      961 tatttcggcg ccaatgccgg gcctggcgtc ctgcgtccgg cctacattgc ggaggtcctg
     1021 gccgccgcga ggtcatgggc tcttccggcg gaaggcgtgg cggctctgga gagattagcc
     1081 caacggtagc cgtctatgat ggcaagcgcc ccgatcgccc ggccgctaac cgttcagaaa
     1141 ctgaacgttc tgcgacgatt gctgttctgt tcatgaacag cctccgcccc ctttatatct
     1201 taaagtatct gatttcattg ccgattacgg attggtccgt aacttgccac ctcactatcc
     1261 ggctcagcac cgacccccat accgggtaag gacgagagca ccaaagataa acaacagtaa
     1321 ggtgagggaa ctatgcgcaa gatgatgaag gccgctattt tcgttgagaa ggggcggatc
     1381 gtcctcgatg acaaaccgat ccctgatatc ggtccgcagg acgcactgat ccgagtcacc
     1441 accacaacca tctgcggaac cgacgttcac atcctcaagg gcgaatatcc cgtagccaag
     1501 gggttgacga tcgggcacga gccggtcggc gtcatcgaga agttgggctc tggcgtggtc
     1561 ggcttcaaag aaggccagcg cgtgatcgcc ggcgcgatta ccccgtcggg gtggagccac
     1621 gcctgtctat gcggttgcgg ctcgcaggat ggagctggca cgaagcatgg ctggcaggcg
     1681 atcggaggct ggaagttcgg caacacgatc gatggctgcc aagccgaatt cgtgtgcgtt
     1741 cccgacgcaa tggccaatct ggcgccggtc gatgacgcgg tgagcgacga gcaggtcctg
     1801 atgtgcccgg acatcatgtc cacgggtttt gcgggagccc aaacagggga aataaaaatc
     1861 ggcgatacgg tcgcagtttt cgcacagggg ccgattgggc tctgcgcaac cgcgggggca
     1921 aagctcctgg gcgcgacaac cgtcatagga gtcgacacgg tgcctgcgcg actcgccatg
     1981 tccaaacgca tgggcgccga tcacgtcgtc gactttcgca aggaagatcc ggtcgaagcc
     2041 atcatgcggc tgaccgatgg ccgcggcgtc gatgtcgcca tcgaggcgct gggaacccaa
     2101 gccacgttcg aggcggcgct cagggtgatc cggcctggcg gcgtcctgtc cagtttgggt
     2161 gtctattcct cggatcttaa gatccccctg gacgccttcg cggcgggact cggcgacaaa
     2221 tcgatccgca cctcgctctg tcccggcggc aaggaacgca tgcggcgtat gttggcggtg
     2281 atttcgtccg gccgcgtgga tcttggggga ttagtcaccc atcgcttcaa actcgatcag
     2341 atcgaggaag cctatgactt gttcgccaac cagcgcgatg gcgtgctcaa ggtggcggtc
     2401 tcggtttaga cgaaggctca cgaactggcc ccgcggctcg aggccgccga ggcttggcca
     2461 gctttagatt ctgcgctcag tcaatcgcaa ggatgtttgc tggcggtcgt gacgaggaaa
     2521 accggaccag agacctcatt gttcgatcgc gtccgtttgg ccgctgtcac tcacgttggc
     2581 cagacggttg tcgaactcgt atgcgtagcc gacgcggaat agcgctcccg ttgatcgcct
     2641 gcgtgaagca tcaattcgac gcggccgtcc ccggctcggc gccggtggcg ccgccgaggc
     2701 tgagccacac atttggatcg tcctgggctt ggcgcttgta gaactgatag ccagtggcgg
     2761 tccacggccg gatctcatcc gacagattgt cgagcacgaa gtcgccgcgg tcggtcttga
     2821 ccgtgagcac cgcatggccc tcgccattga ggtcgcgaac gatggtcatc aacagggcct
     2881 ggcgcggaaa gccgcggtcg atgagcagtt tgcgcttgaa cagcgcatag atcttgcagt
     2941 cgcccttgcc atcgaccgga tagtcccaat ggtcgagcgt cgtcccccaa tggtcgaagt
     3001 tgctgatcgg ctcgatcgcg ttgttcaccc ggttgttgat ctcgttgaga accttccagc
     3061 tttgccgggt caggcgcacg tccatgggct tgagcttgcc gggcgtgcac tcctcgctgt
     3121 gccgattgca gaagtcgacc cagccgtagg ggatcgaggc ttcgccgcca accggcgcgc
     3181 gagtggcctg gggaaggccc tgttccgaag cttcgacgaa ggtgttccag gcgaagccga
     3241 agaccaccgc cgcagccaca aagcgaaaga gccgagagag cattgggttg tccgccaggt
     3301 tgaatgggga cactatcggc gccggcgctt ggcggccgag aaacaaaatc ggttcaattt
     3361 tatcgatcgg cgaaagttgg gcgggcgaaa ttcattaacg ttgacggagg gtaatctcca
     3421 tcagcgagcc gccctcggac gtcgcgctgg ccctggttcc aacgggcgta gaaagcctgt
     3481 caacaatctg tttcaacagg cgcttatcga aggacatccg gacggctccg tggccggcgg
     3541 tcgatggaga gccgagggag agcgggatgt cgaccgaagc aaccagcgcc gaaccccaat
     3601 cgattgggcg acttggtctt atggggctcg cggcgctcgg cgtcgtgttc ggcgacatcg
     3661 gcacaagccc gctctacacg ctgaaaaccg tgctcgacta caccgggcgg catcctgacc
     3721 ctgcagccac gctcggcgcc ttatcgttga tcttgtggac gctcttcctc atcacgacgg
     3781 tcaagtacgt caatatcgcc atgcgcatcg acaacgacgg cgaaggtggc atcatcgccc
     3841 tgatgaccct gctcggcgtc aagcagcatc aacggccgct gattatcgca gtgggcctgt
     3901 tcggcgcggc gctcatttac ggcgatggcg cgatcacgcc tgcgatttca gtgctctcgg
     3961 cgctcgaagg ggtggagcag atcgcgccga gtttgcagac ctatgtgctg cccgcagcga
     4021 ctgtggtcct gctggcgctg tttgcggtgc agccattcgg gacggcgaag atcggccgcg
     4081 cgttcggccc gatcatgctc atttggtttg cgtcgatcgc ggccttgggc gtctatggcg
     4141 tcctgcagca cccgtcggtc tttgcggccg tcgatccacg ctacggcctc gactatctgt
     4201 cgcggggcgg attggaggga ttcctggtcc tcggcggcgt ctttctttgc gtcacggggt
     4261 ccgaggcgct gtatgcggac atggggcact tcgggccaaa gccgatccgc gcgtcctgga
     4321 tcctgatcgt ctttccggct ctcattctca actacgccgg ccaggcggca attgtgctcg
     4381 agggggcgcc gacccagggc aacatcttct accggctctg ccccgggccg ctgctgacgc
     4441 cgctcatcgt gctggcgacg atcgccaccg tcatcgccag ccaatcgatc atcaccggcg
     4501 ccttttcgat gacccgccag gcgatgcaac tcggctggct gccgcgcatg aacatcactc
     4561 agacctcgga ggaaggctac ggccagattt acgtcggagt ggtcaactgg ctgctcatga
     4621 tcgtcaccct cggactgacg atcggtttcg gcaagtccga caatctcgcc gccgcctacg
     4681 gcatcgcggt ctc
//
LOCUS       DATAQL010000954         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4677447, whole genome shotgun sequence.
ACCESSION   DATAQL010000954 DATAQL010000000
VERSION     DATAQL010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4677447"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /gene="rnpA"
                     /locus_tag="VHS97_16600"
     CDS             complement(<1..328)
                     /gene="rnpA"
                     /locus_tag="VHS97_16600"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX3449876.1"
                     /translation="MKIKATFRPHERLKDPLDFRRAFDRRRSSADDVLVIYAVENGRD
                     YARLGISLSRKKVRAAHARNRLKRLVREAFRLSKSELPTGVDLVVLPRRQDASFAAIR
                     QSLVALA"
     gene            complement(605..931)
                     /locus_tag="VHS97_16605"
     CDS             complement(605..931)
                     /locus_tag="VHS97_16605"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEX3449877.1"
                     /translation="MTRRIIPREIVARELSVSTQVLTRYESLGFVRAIHDGPLEGYEP
                     AQIRRLWSIVSYQRDMGINLAGVEVILQLVDHLSQVRHRVDDLAHEFRALLDEDDSPP
                     ASNTNE"
     gene            complement(955..1923)
                     /locus_tag="VHS97_16610"
     CDS             complement(955..1923)
                     /locus_tag="VHS97_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX3449878.1"
                     /translation="MAERDYYDILGVARDATPEAIKKAYRGLARKFHPDVNPGDKTAE
                     SRFKEVQSAYDVLSDQEKRALYDRHGRSAFEGMAASGPRASATEWASRFGEPGFEAVD
                     LSDLIGSFGTSSRGEDAGGASIFEDLMGRVRGARPSRQRAGRTLEAHLTIPFLTAVRG
                     GETTIEVQRGPNKTESLVVKIPPGIDTGAKLRLKGQGEQGSKGTPAGDLTITIEVEPH
                     PYFKREGQNLVLEVPISVSEAILGAKIDVPALDGMKSLTIPPGSSSGLKLRLKGQGIP
                     TSGSKAGGDLFVVLKILVPKTIDAASRRLIEEFAERNPQHPRAGLW"
     gene            2234..>2494
                     /locus_tag="VHS97_16615"
     CDS             2234..>2494
                     /locus_tag="VHS97_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449879.1"
                     /translation="MKRVAVILHERLGHWNRQLRPRLHDQRIRWFETRSRGDLDNLLI
                     GLSCPVVVIDLGRQPTLGLQDLMRVLERAPDARVLVLDPESHG"
BASE COUNT          459 a          747 c          774 g          514 t
ORIGIN      
        1 cggccaatgc gacgagtgat tgccggatgg ccgcgaaact cgcatcctgc cggcgcggca
       61 gaacaaccag gtcgacaccg gtcgggagct cggacttgct aaggcggaac gcttcacgga
      121 cgagccgctt caggcggttg cgcgcatggg ctgcacggac cttcttacgc gacagtgaaa
      181 tcccgagccg cgcatagtct cgcccgttct cgactgcgta gatcacgagc acgtcgtcgg
      241 cgctcgagcg acggcgatcg aaggcgcgac gaaagtcgag gggatctttc agccgctcat
      301 gaggccggaa cgtcgctttg atcttcatga tgtcgggtcg acctcatggc cgtccgcgtc
      361 cggaagatgt acaatgcggg gcgcgtttag gcgctcacga atcaagcgac cgcgccgcct
      421 gatgtgccag atcaatagcg cgccgaacac ggcgacggcg accagaaatg tacccagcaa
      481 ggcggcgaca gcccggccca acctcgagcg ggcctccgga cgctcttcga gtatccgcga
      541 aagctggctc cgtcgggatt gatccccggc gagcgcccgg tctgttggcg ctggatttgt
      601 ggtgtcactc attggtattg gatgccggcg gtgagtcgtc ttcgtcgagt agagctcgaa
      661 actcatgggc aaggtcgtcg acgcggtgtc gcacctgcga gagatggtcg acgagttgca
      721 gaattacctc gaccccggcc aggttgattc ccatgtcgcg ctgatagctc acgatcgacc
      781 acagccgacg aatctgcgcg ggttcataac cctccaatgg gccgtcgtgg atggcgcgca
      841 cgaagcccag agactcgtac cgagtcagta cttgggttga gaccgaaagt tctcgtgcga
      901 cgatctcgcg cgggataatc cgtcgagtca tgaccgagtg ctcccgttcc cagttcacca
      961 gagtccggca cgcgggtgct ggggattgcg ttcggcgaat tcctcgatca accgccggct
     1021 ggccgcgtcg attgttttag gtaccaaaat cttgagtacc acgaacaagt cgccgccggc
     1081 cttgctcccg ctggtgggga taccttgccc cttgagccgg agtttgagtc cgctcgagct
     1141 gccgggcggg atcgtgagcg atttcatccc atcaagggcg ggtacgtcga tcttagcccc
     1201 gagaatagcc tcggagacgc tgattgggac ttccaggacg aggttttgac cctcgcgctt
     1261 gaaatacgga tggggctcga cctcgatcgt gatcgttaaa tcgcccgctg gcgttccttt
     1321 tgacccctgt tctccctggc ccttgagccg gagtttggcc cccgtatcga taccgggagg
     1381 gatcttgaca acaaggcttt cggtcttgtt cgggccgcgc tgcacctcga tcgtggtttc
     1441 gccgccgcgg acggcggtca aaaatgggat tgtcaggtgg gcttcgaggg tgcgaccggc
     1501 gcgctggcgg cttggccggg cgccgcgcac ccggcccatc aagtcttcaa agatgctcgc
     1561 tcctccggca tcttcgccac gcgacgatgt gccgaaagag ccgatcaagt cagaaagatc
     1621 aacggcttca aacccgggct cgccaaacct ggaagcccat tccgtagcac tcgcacgcgg
     1681 tccggacgcc gccattcctt cgaaggcgct ccggccatgg cgatcgtaaa gggctcgctt
     1741 ctcctggtcc gagagcacat cgtaagcact ctgcacttcc ttgaatcggc tctcggccgt
     1801 cttgtcgccc ggattgacgt cgggatggaa cttgcgtgcc agtccgcgat aggccttttt
     1861 gatggcctcg ggcgtcgcgt cgcgagctac tcccaagatg tcgtaataat cgcgttccgc
     1921 catcgagcga ccggttccaa attcgtatgc gccagcgtgt tatcgatcac gctcaatatc
     1981 gagttgcatc aaccacgaat tataagcctg aatcggccgg agattcaagc gagcgcttgg
     2041 atgtatcgaa ttctagctac cagatcgtgt gctgacgagt cggtccatcc tgaagagcct
     2101 tgctcccttt aaacgccgcg acatgttcga ccctcgatgg tcgcaggctt ccgcggtaca
     2161 atgttttgag cggtttagga tactcaagag tcgagactgg gatcgttcaa tttcgggcaa
     2221 gcgagggctg atcttgaagc gcgtcgcggt catcttgcac gagcggctgg gccattggaa
     2281 caggcaactg cgtccgaggc tgcacgatca gcggatccga tggttcgaga cgcggtcgcg
     2341 gggcgacctg gacaatctgc tgatcggatt gtcctgcccc gttgtggtca ttgacttggg
     2401 gcggcaaccc acgctggggt tacaggacct gatgcgcgtg ctcgagcgtg ctccggatgc
     2461 tcgggtcctc gtgctcgatc cggaatccca cggc
//
LOCUS       DATAQL010000955         8950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_557319, whole genome shotgun sequence.
ACCESSION   DATAQL010000955 DATAQL010000000
VERSION     DATAQL010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557319"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VHS97_16620"
     CDS             complement(<1..500)
                     /locus_tag="VHS97_16620"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3449880.1"
                     /translation="MFPQAEDLARYWANIKRGIDAITEVPSSHWRVADYFNEDPKARD
                     QTYAHKGGFLTPVDFPLLDFGIAPNSVEATDTTQLLGLMVARHALADAGCLSSETLDR
                     DKVSVILGVTGTLELVIPLGARLGHPIWRQALQSAGVDPTTTEDVVRRIADSYVGWQE
                     NSFPGLL"
     gene            complement(642..2231)
                     /locus_tag="VHS97_16625"
     CDS             complement(642..2231)
                     /locus_tag="VHS97_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PfaD family polyunsaturated fatty
                     acid/polyketide biosynthesis protein"
                     /protein_id="HEX3449881.1"
                     /translation="MVVTSANVESLEDALGRIGKSFSLGIRSGGALVIDDQRVGKSSS
                     QNGEAAAWVPACRLEDLGDASFKNDHGLRYALMSGAMANGIGSVEIVESMGKRGFLGI
                     FGSAGLSLSAIEQTIDRIQSWTDNSIPYGMNLIHSPGEPEMEAAVVDLYLRRGVRLVE
                     ASAFLNLTAPVVRYRVAGLRRDGSGNIVAANRMIAKVSRVEVASKFMAPAPPQILRAL
                     VASGEVSAEQAEWAARIPLADDITAEADSGGHTDNQPAIVLLPTMLSLRDRIQETHRY
                     ERSLRVGAAGGISTPWSAAAALAMGAAYLVTGSINQSCIEAGTSAAARSMLAQARQAD
                     IAMAPAADMFEMGVKVQVLKRGTLFAMRSAKLYELYRTHDGLDQIPDGERASLEKTIF
                     RAPLEAIWDQTKAFFQERDPARIERSNDDPKQKMALVFRWYLGMSSYWATSGDPGRTV
                     DYQIWCGPAMAAFNDWVRGSFLEAPENRRVAAVALNILYGAAVLTRARIASIQGVRLP
                     AGVPRLIPLDTTEIENRLFLS"
     gene            complement(2495..3211)
                     /locus_tag="VHS97_16630"
     CDS             complement(2495..3211)
                     /locus_tag="VHS97_16630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3449882.1"
                     /translation="MGYDKSQVAQEFTSWSTSYDRSILQWLLFAPSHRVMIRRIREAA
                     GDRPVKMLDVGCGTGLFASKVRDSLPHVEVCGVDLVSEMLAKGQTRWRLHRAHVLPVQ
                     GDSERLPFASGSFDIVTCANSFHHYPHQDRAVAEMGRVLRPGGRLMIIDGYRDGLWGW
                     FIYDVCVAGIEGEVHHASARRFRDLMTEAGLQAIAQRVFRGPAPFLITEAIATEPVPV
                     VPPPHFRLGYFATGEHQPLD"
     gene            3483..4667
                     /locus_tag="VHS97_16635"
     CDS             3483..4667
                     /locus_tag="VHS97_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3449883.1"
                     /translation="MRPNPVYLDNHATTRTDPRVVEAMIPYFTELYGNSASTSHRFGS
                     QAAEAVERARAQVARALGAEPREIVFTSGATESNNLAIKGVARAATRRGNHLVTAASE
                     HRAVLDPFKRLARQGWEITVVPCDENGRVSAAAVAVALTERTVLVSIMAANNEVGAIN
                     PIAEIGRLCHDHGVVFHTDAAQAVGKIPLDVQADSVDLLSLSAHKIYGPKGIGALYVR
                     RRDPQVRLEPLVDGGGHERGLRSGTLPVPLVVGLGLAAEIAMRERDDEARRLLELRER
                     LHQGIAGRVPGIRLNGPPADRLPGNLNLSFGDVDGEALMMAMRDIAVSSGSACSSAEP
                     EPSHVLLAIGLDEDMARASLRFGLGRFTTTDEIDFAIDTVAAAVERLRTQTVAWSVRD
                     GA"
     gene            4753..5082
                     /locus_tag="VHS97_16640"
     CDS             4753..5082
                     /locus_tag="VHS97_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX3449884.1"
                     /translation="MGVSLTEKAASEVKKIITEQNLPDGTVLRVGVQGGGCSGFSYSL
                     GFDTATSERDRVIEAHGVRMVVDKKFDPYLDGTVVDYYDGLEKRGFVFNNPNVVKSCG
                     CGSSFQV"
     gene            complement(5805..6788)
                     /locus_tag="VHS97_16645"
     CDS             complement(5805..6788)
                     /locus_tag="VHS97_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX3449885.1"
                     /translation="MPRVLIGPYLLRNQPGRFRQILTDAGFEPVDPEGDFSLSKLQLL
                     PHLPEIDAMLAGGERMTSELFALAPRLRAIARTGVGYDLIDLAAATQHKVAVTITPGT
                     NHESVAEQAMALLLALAKRVIHNDRVIHSGGWDRALVTPMRGRTMGLIGMGRIGRAVA
                     VRALAFGMAVVAFDTVVDAEFDKQHGIKRLELVELLASSDVVSLHLPLTDATRGMVNR
                     DFLARMRPGSFLINTSRGGLIVETDLRDALVSGHLAGVGLDVLNHEPPEPGNPLIGLA
                     NVVLSPHIAGTDTQSMREMAELAATTIVELSQNRWPANCVVNSELREGWRW"
     gene            7147..8628
                     /locus_tag="VHS97_16650"
     CDS             7147..8628
                     /locus_tag="VHS97_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX3449886.1"
                     /translation="MKFRLPSSEPAHRLPDYRKAYITGLDRTPGRLAVEMRNGMISCV
                     RDSPESGRLFVPWPIEGHGTPIVGTATLAERSAPYMLAVELARGKLNDIRNQLSDWVQ
                     MGLRSTPELEEALAQAQHAFVRCATSTDDPDLCTEAARKSLQAASLAGDLLIDAYTAQ
                     ILQSRLSATSRLSTHVGCVVDFPPQKLPPSIDWPSAFNTGHLGISWKQVAPSEGQYRW
                     DVLDAQLAWCRKIGLTSEVGPLVEFRQGALPDWIWLWEGDSETISGLVADFVRQTVQR
                     YRGRVPLWHVVHRPASGEILGLTEEEQIRITARAVQVAHQADPSAQLTIGIDRPWAEW
                     MGSSHFQLGPLHLCDYLLRAELGLSGVAIEIAPGFSSPGSHMRDLFELSKLLDLYSLL
                     NVPLHVWMALPSGAGPDPNAEPLVKVEPAQWPASLDDSLQAAWGAKWIALVVSKPFVR
                     TLSWLQLSDAFPHLFPHAGLLRPDQTAKPLVAWLKMLRKETLA"
BASE COUNT         1720 a         2730 c         2839 g         1661 t
ORIGIN      
        1 aggaggccgg ggaatgagtt ttcctgccag cccacatacg agtcggcgat ccgccggacg
       61 acatcttcag tcgtggtcgg gtcgacgccg gcggactgga gggcctggcg ccagatgggg
      121 tgacccaatc gggcgcccag cgggatgacc agctcaagcg tgccggtgac gcccaggatg
      181 acactgacct tgtcgcggtc gagcgtttca ctggagaggc agccggcatc ggcgagtgcg
      241 tggcgggcca ccatgaggcc gagcaactgg gtggtgtcgg tggcttcgac agaattgggg
      301 gcgatgccga aatcgagcag cggaaagtcg actggagtca gaaatccgcc cttatgggcg
      361 taggtttggt ctcgggcctt agggtcttca ttgaaatagt ccgcgacgcg ccagtgggaa
      421 ctaggcactt cggtgatcgc gtcgatgccc ctcttgatat tggcccagta gcgtgcaagg
      481 tcctccgcct gaggaaacat gcatcccatt ccgatgatgg ccaccggaac cggaggcggg
      541 aaatcggacg aattcaagtc tctacccttt cgaacgacgt ttaagaccag cgtgggccgc
      601 gggtttcgcg ggcggcgaat cttggcgatg atcgtggtat gttaggataa gaacaaccgg
      661 ttctcaatct cggtggtgtc cagtggaatc aaacggggca cgccggccgg cagacggacc
      721 ccctgaatgc tggcgatccg ggctcgggtc aagacggccg cgccataaag gatgttcagg
      781 gcgacggccg ccacccgacg attttcgggg gcctccaaga aacttccgcg cacccaatcg
      841 ttgaaagcgg ccatggcggg cccgcaccag atctggtaat cgacggtccg ccctgggtcg
      901 ccgcttgtgg cccagtacga cgacatcccc aagtaccagc ggaagacgag agccatcttc
      961 tgcttgggat cgtcgttcga tcgctcgatc cgcgcggggt cgcgctcttg aaaaaaggcc
     1021 ttggtctggt cccagatcgc ttccaagggg gcgcgaaaga tggtcttctc gagcgatgcc
     1081 cgttcgccgt cggggatctg gtcgagaccg tcgtgcgtcc ggtagagctc gtaaagcttg
     1141 gcggagcgca tggcaaacaa ggtgccgcgc ttgagcacct ggacttttac gcccatctcg
     1201 aacatgtcgg ccgcgggggc cattgcgatg tcggcctggc gcgcctgcgc cagcatactc
     1261 cgagccgcgg cggatgttcc tgcctcgatg cacgactggt tgatcgatcc ggtcacgagg
     1321 taggctgccc ccatcgccaa ggcggcggcc gccgaccacg gagtggaaat gcctcctgcc
     1381 gcgccaacgc gtagcgagcg ttcgtagcgg tgcgtctcct ggatccggtc gcgcaaactc
     1441 agcatggtcg gcaagagcac gatcgccggc tggttgtcgg tgtggcctcc ggaatcggcc
     1501 tcggccgtga tgtcgtcggc taacggaatc cgcgcagccc actccgcttg ctcggccgaa
     1561 acctcgcccg aggcgacgag cgcacggaga atttgcgggg gggccggcgc catgaacttg
     1621 gatgcgacct cgacccgcga aaccttggcg atcatgcgat tggccgcgac aatattcccc
     1681 gaaccatcgc ggcggaggcc tgccactcga taacggacca ccggcgccgt caggttgagg
     1741 aaggcagatg cctcgacgag ccgcaccccc cgcctgagat agagatcgac gacggccgcc
     1801 tccatttcgg gctcaccggg gctgtggatc aagttcatcc cgtacggaat cgaattgtca
     1861 gtccaggact gaattcgatc gatggtttgc tcgatcgcgc tcaatgaaag ccctgccgag
     1921 ccaaagattc ccagaaatcc gcgctttccc atcgattcga cgatctcgac cgacccgatg
     1981 ccattggcca tcgcgcccga catcagtgca tagcgcaatc cgtgatcgtt cttgaaactc
     2041 gcgtcgccga gatcctcgag tcgacaggcc ggcacccacg ccgccgcctc gccgttttgg
     2101 ctcgagctct tgccgacccg ttgatcgtcg atgactaggg cgccccccga ccggatcccc
     2161 agcgagaacg acttgccgat ccgaccgagt gcatcctcca agctctcaac attcgccgag
     2221 gtcacaacca cggagtcgct ccaaacccag tcagccaatc aagcatggtg gaaaattgtc
     2281 gtgcgtcgcg cggtacgacg gttattggcg agtccgtcgc attgcatcta gaggaaaagc
     2341 gcctcgatag caagtggtcc gctcgaggac gaaccctgga atcccgcaat attggacagg
     2401 gcacgacgga tatctctggc gggacatggg atcaaagcct caaggtagat cgcggggtgt
     2461 taattcgtcg gtgtttgatc gctgcttctc gaccttagtc gagcggttga tgttcccctg
     2521 tggcgaagta gcctagccga aaatggggcg gaggcactac gggaaccggc tcggtggcaa
     2581 tggcttcggt gatcagaaag ggagccggac cgcggaatac gcgctgggcg atggcttgca
     2641 ggccagcctc ggtcatcaga tcgcgaaacc tgcgcgccga agcgtggtgg acctctccct
     2701 cgatgccagc gacacacacg tcgtagatga accaacccca tagtccatct cgatatccgt
     2761 cgatgatcat tagcctgccg cccggccgta agacacgccc catctcggcg accgcccgat
     2821 cctggtgggg atagtggtga aaactattgg cgcaggtgac gatgtcaaag gatcctgagg
     2881 caaacggcag tcgttcgctg tctccctgaa cgggtaagac atgcgctcga tgcagtcgcc
     2941 agcgggtttg gcccttcgcc agcatctcgg acacaagatc gacgccgcag acctcgacat
     3001 ggggtaagga gtctcggacc ttggatgcaa acaagcccgt cccgcagccg acgtcgagca
     3061 tcttgaccgg acggtcgccc gcggcttcgc ggatccgcct gatcatgacg cgatgcgaag
     3121 gagcaaatag gagccactga aggatgctgc ggtcgtagct agtgctccaa ctggtgaatt
     3181 cctgggccac ctggctctta tcgtaaccca tgccatggcc tccttgccgg gcgaatccgc
     3241 gaggtcagac aaagtcgcgc tgggccgggc ttccttcggc cgggcctgtg acttttagtc
     3301 tttaacgaat gtcggagtat cccgcaaccc ggaattgacc cagatcttga tcatttcgca
     3361 tcttacggag cgagtggaaa gacggctacc ggcagcgtat gatccatcgg gggaataaat
     3421 tttggcggag tccgagcttt tttcgacacg caaaccagtt gccgagccaa aggtagaatc
     3481 gcgtgcggcc caacccggtg tacctcgaca atcatgcgac gacgcgtacc gatccccggg
     3541 tcgtcgaggc catgattcct tatttcactg agctttacgg caactcggcc agcacgtctc
     3601 atcggtttgg ctctcaggcg gccgaggccg tcgagcgtgc gcgagcacag gtggcccggg
     3661 cgcttggcgc cgagccgcgt gagatcgtct tcacgtcggg cgcaacggaa tcgaacaacc
     3721 tggcgatcaa gggagtcgcg cgcgccgcga cccggcgcgg caatcatctc gtgacggcgg
     3781 cctccgagca cagggcggtt ctcgacccgt tcaagcggct ggcgcggcaa ggctgggaga
     3841 tcacggtcgt tccgtgcgac gaaaacggac gcgtttcggc cgcggccgtc gccgttgcat
     3901 tgaccgagcg cacggtcttg gtatcgatca tggccgccaa caacgaagta ggggccatca
     3961 atccgatcgc cgagatcggc aggctctgtc acgatcatgg cgttgtgttc cacaccgatg
     4021 ccgcacaggc ggtcggcaag ataccgctcg acgtccaggc ggactccgtc gacttgctga
     4081 gcttgtccgc gcacaagatt tacggtccca aaggaatcgg cgctctttac gttcgccgcc
     4141 gcgatcccca ggtccggctc gaaccgctgg tcgatggggg ggggcacgag cgcgggctgc
     4201 ggagtgggac gctgccagtg cctctggttg tggggctggg tttggcggcc gagatcgcaa
     4261 tgcgcgagcg cgacgacgag gcacgccggc tgctcgagct gcgggaacga ctgcaccagg
     4321 ggattgccgg acgcgtcccg ggcatcaggc tcaacgggcc gccggccgat cgcttgcccg
     4381 gtaacttgaa cctcagcttt ggcgacgtcg acggcgaggc gctcatgatg gcgatgcgcg
     4441 acatcgccgt cagctcgggc tcagcgtgct cgtcggccga acccgagcca agccatgttc
     4501 ttctggcaat aggtctcgat gaagacatgg cccgggcaag cctccggttt ggccttggcc
     4561 ggttcacgac taccgacgag atcgatttcg cgatcgacac cgtagcagcc gctgtagagc
     4621 gcctgcgaac gcaaaccgtt gcgtggtcgg tacgggatgg cgcatgattt gacttcacgg
     4681 tatgatacat tatcgcgatc acttactcgg gaatcgactg gcagagttcc cacacacaca
     4741 ggagattatt cgatgggcgt gtcactgacc gaaaaggccg cgagcgaagt caagaaaatc
     4801 atcaccgagc aaaaccttcc cgatggcacc gtcctgcgcg tcggtgttca ggggggcggc
     4861 tgcagcggtt tttcctatag cctgggattc gacacagcga cctccgagcg cgaccgcgtc
     4921 atcgaggcgc acggcgtacg catggtggtc gacaaaaaat ttgacccgta cttggatgga
     4981 accgtcgtcg actactacga cggtctcgag aagcgcggct tcgtgttcaa caaccccaac
     5041 gtcgtcaaga gctgcggttg cggcagttcg ttccaggttt gagctcttgg agctgcacgt
     5101 tgctagtgcc gggttactga ccccgtcggc cactgcttgc gcacgctctc cccgccgcgg
     5161 agcttcgagg gaggggcttt cgttaccact agctccctct cctttgggag agggctgggg
     5221 tgaggggttg attgcagcaa tacctccgct cgatcgaatg actcgccacg agacaccatg
     5281 atcagcgtga gagccgtcgc gccgccagct acgagctccc gctcttcaac gagagtggcg
     5341 ggaggagggg cgggttgatg aaggaacgag aatcgccgag aatgagagcg aggagacaga
     5401 atccttctcc aaaggaaaag cctgggaatc gcttactctt acagattccc agagtgaggc
     5461 tttcctccgg gagcagcagc ggcttcgggt cagcggtgac gatgtcctga aggagaccga
     5521 gtctgtgctg tttgcgatct tacaagcggc cggaaagaaa gtggtttgag tgagatcgag
     5581 cgcaacaata cctctttaga gagaatgcgc atcgggcgaa tcaacaactc cctggtagga
     5641 gtcaggaaag ctcctccatg tgagccgccg ggaggggagg acgaaaggca agacctctcc
     5701 ctagaacgtg gcaagggcat cttgcccgtg tccgggcgca ttacgggcgg aatcgcgtgt
     5761 gattcccggg ctggagcgag gggccgtgat actccggcaa ccaatcacca ccgccagccc
     5821 tcgcgcaatt cggaattcac gacgcaattc gccggccagc gattctgcga cagctccacg
     5881 atggttgtgg ccgccagttc ggccatttcg cgcatcgatt gcgtatccgt gccggcgatg
     5941 tgggggctca acaccacgtt ggcgagtccg atcagcggat tgcccggctc gggcggctcg
     6001 tggttgagca cgtccaatcc gacgccggcg agatgccctg ataccagggc gtcacgcagg
     6061 tcggtctcga cgataagacc accgcgcgac gtgttgatca gaaaagagcc cgggcgcatc
     6121 cgcgccagga agtcgcgatt gaccatgccg cgcgtggcat cggtgagcgg gagatgcagg
     6181 ctgacgacgt cagagcttgc cagcaactcc acaagttcca gccgtttgat accgtgttgc
     6241 ttgtcgaact cggcatcaac gaccgtgtcg aacgcgacga ccgccatgcc aaatgccagc
     6301 gcacgcacgg cgacggctcg gccgatgcgg cccatgccga tcaggcccat ggttctaccg
     6361 cgcatcggcg tgaccagggc acggtcccag ccgccggagt ggatcactcg atcgttatgg
     6421 atcacgcgct tcgccagcgc gagcaggagg gccatggcct gctcggcgac gctttcgtga
     6481 ttggtccccg gagtgatcgt cacggcgacc ttgtgctgag tcgcggctgc cagatcgatc
     6541 aggtcgtagc cgaccccggt gcgcgcgatc gctcgcagac gcggagcgag cgcgaaaagc
     6601 tcgctcgtca tgcgttcgcc acccgcgagc atcgcgtcga tttcgggaag gtggggaagc
     6661 aattgcaatt tggacaggga gaagtccccc tcggggtcga ccggctcgaa gccggcgtcc
     6721 gtgaggatct ggcgaaaccg gcccggctgg ttccgcagaa ggtagggacc aatcaaaact
     6781 cgaggcatgg tcagctctgc aagattgtgg ttattataac gaattaccga gatccggatc
     6841 gcggcttgcg ctgttacatt agcgaacgac cgtcagccgt cacttgcgtt gttcaggtgc
     6901 ctaacttatt ctcatcggac tcattcggca accaggcagt gccggcaagg ggccgcccta
     6961 aatcacgcga acggtaggaa gagccccttc gtcgagtcct agtccagggt gatcagcaca
     7021 gagccgagag atcgcgggcg agtgccaatg tgtaccgtaa gaatgggcgg ccggccagga
     7081 tttggttggt ctatttaatt gtgaagcggt ctcgtcccgc tgcgcggaaa gcgtgccatg
     7141 ggggtaatga agtttaggct gccctcgagc gaaccggctc accggcttcc cgattatcgc
     7201 aaggcataca tcaccggcct cgatcggacg ccaggacggc tggcggtcga gatgcggaat
     7261 ggcatgatca gttgcgttcg agatagcccc gagagtgggc gcctttttgt cccttggccc
     7321 attgaagggc atggcactcc gatcgtcggg accgcgacgt tggccgaacg gtctgcgcct
     7381 tatatgctgg cagtcgagct tgctcgcggc aaactcaacg atatccgcaa ccaattgtct
     7441 gactgggtgc agatgggact gcgctcgacc cccgagttgg aggaggcact ggcgcaagcg
     7501 cagcatgcgt ttgtccgctg tgcgacctcg acggatgacc ccgacctatg tacggaggct
     7561 gcccgaaagt cactgcaggc ggcatctctg gccggggatc tgttgatcga cgcgtacaca
     7621 gcccagatcc tccagagccg gctgtcggcg acgtccaggc tctcgaccca cgtcggctgt
     7681 gttgtcgact ttccacctca aaaactaccg ccgtcgatcg actggccgag cgccttcaac
     7741 acgggccacc tgggcatatc ttggaagcaa gttgctcctt ccgaagggca gtatcgctgg
     7801 gacgttctcg acgcccaact ggcatggtgc cgtaagatcg gcttgacatc cgaggtcggc
     7861 cccctggtcg agtttcgcca gggcgcgcta cccgactgga tctggctctg ggaaggcgac
     7921 agcgagacga tctcggggtt ggtggccgac tttgttcggc agaccgtgca gcgctaccgt
     7981 ggccgagttc cgctctggca cgtggtccac cggccggcca gtggcgagat cctgggtttg
     8041 accgaggaag agcagatccg gatcacggcg cgggccgtcc aggtcgccca ccaggccgac
     8101 ccgtcggctc agctgacgat cggcatcgat cgtccctggg cggagtggat gggctcgagc
     8161 cactttcagc tcggtccgct gcacctttgc gattatttac tccgggccga gctcgggctt
     8221 tcgggcgtgg cgatcgagat cgcccctggc ttttcttcgc ccggcagcca catgcgagac
     8281 ctgttcgagc tgtcgaaact cctcgacctt tactcgctgt tgaacgttcc gctccacgtc
     8341 tggatggcac tgccatccgg cgccggcccc gaccctaatg ccgagcccct cgtgaaggtc
     8401 gagccggcgc agtggcccgc ctcactcgac gacagcctgc aagccgcctg gggggcaaag
     8461 tggatcgccc tggtcgtctc aaagccattc gttcgcacgt tgagctggtt gcagctcagc
     8521 gacgcctttc cccacctctt tccgcacgcc ggccttcttc gccctgacca gaccgccaag
     8581 ccgctcgtgg cctggttgaa aatgttgcgc aaggaaacgc ttgcgtaggg tggcgccgag
     8641 ccgcgacaaa tcgcggcgag gcccaccgac ctgccgtggg ccgagctgcg ataaatcgcg
     8701 gcgaggccca ccgacctgcc gtagaccgaa ccgcgggaaa tcgcggcaag gtccaccgac
     8761 ctgcggcggc tcactcgggc ggtttttgaa ccacaggatt ggcggcggca aggatttcgc
     8821 ccgcttaatc aaaattcgca gccgcgccga catctccgca gcgacctcga taccggtcag
     8881 acctgcgccc acgacgacaa ccgtgaaacg ccccgatgac tcgggttggc cggtcagcat
     8941 ctccaagtgc
//
LOCUS       DATAQL010000956         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4067548, whole genome shotgun sequence.
ACCESSION   DATAQL010000956 DATAQL010000000
VERSION     DATAQL010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4067548"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..707)
                     /locus_tag="VHS97_16655"
     CDS             complement(117..707)
                     /locus_tag="VHS97_16655"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX3449887.1"
                     /translation="MREPLPLATIHDAVLEFLRGRTDAVLFGAHAVNAYVEESRMTQD
                     VDILSPRAAELAEELRRHLAEKFQIAVRIRTVADGKGHRLYQIRKPKNRHLVDVRAVD
                     GLPAHQVVEEVLVVPPADLIGQKVVGMVSRSGTAKRITDLADIHRLLLAFPELKMSEG
                     VVTERLRAAGAQEPAFNAWREIAAQEISAEDDDEGY"
     gene            complement(895..>3112)
                     /gene="mutS"
                     /locus_tag="VHS97_16660"
     CDS             complement(895..>3112)
                     /gene="mutS"
                     /locus_tag="VHS97_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX3449888.1"
                     /translation="AGGKLGLAWVELSTGRFSLTALLRNELIDEVARLNPAEVLISEL
                     SNDAPWARLLRSQSGMAFTIRHSWDFQPEQARTTLFEQFHTKTLAGFGIDDRSPEVQA
                     AGALMAYLRETQKTALGHITRLIPYRRADTLALDEMTRRSLELVRTLREGKREGSLLS
                     VIDCSATPMGARLLSEWLTSPLTSTELIAERLEAVEELFNHSALRGDLRNLLGQSYDM
                     ERLAGRVGTGRATPRDLVALARTLAILPKLKARLTARVSKRLNQLEAALELCPEIRAE
                     IESALVDDPPLAVKEGGLMRDGYHSGLDELRGSARGGKSWIAKFQAEQVRRTGITNLK
                     VGFNKVFGYYIEITHAQAQARGANIPEDYIRKQTIKNAERYITPELKEYEEKVLRAED
                     RAYELEYELFTTLRDRVSAEAPRLIQAGSVLAQLDVLSSLAELAARHGYCRPEILAEP
                     VFEIEAGRHPVLDARLAPGDFVPNNVELGGKSGSILLITGPNMAGKSTYIRQVALIAI
                     LAHLGSFVPARRARIGIIDRLFARVGATDDLSRNQSTFMVEMTETANILNNATASSLV
                     ILDEIGRGTSTFDGVSLAWAVTEHIHDTIGCRTLFATHYHELVELEKTKSRLKNANVA
                     VREKEGEIVFLYQIVPGGADQSYGIHVARLAGVPAPVLARASEILAFLEKQHGPDPGL
                     PEGPIRKKVKTGRALTGSLFAALPDPLLVELRQIDTTNLTPDGAVELVRRLRELAG"
BASE COUNT          586 a          991 c          934 g          601 t
ORIGIN      
        1 ccccgaaaag tacagagccc gcagaggctc aaaagccgtc gccatgacgg tgatgttaga
       61 atgggccaac ccagtcgatc acggtgacat cgttgacgaa tgtcgtttca ggctgttcag
      121 tacccctcgt catcgtcctc agcggaaatc tcctgagcgg cgatctctcg ccaggcattg
      181 aatgccggtt cttgcgcgcc ggcggcccgc aggcgctcgg tgacgacacc ttcactcatc
      241 ttgagctcgg ggaatgccaa gagaagacga tggatgtcgg ccagatccgt tattcgcttg
      301 gcggtcccgg accggctgac catgcctact accttctgac cgatcaagtc cgccggaggg
      361 accaccagaa cctcctcgac cacctgatga gccggaaggc catctacggc tcggacatcc
      421 acgagatggc ggttctttgg tttgcgaatc tgatacaaac gatggccctt tccgtcagca
      481 accgtacgga tccgaaccgc aatctggaat ttctccgcaa gatgccgccg caactcttcg
      541 gccaactccg cggcgcgtgg cgaaagtatg tcgacgtcct gggtcatccg cgattcttcg
      601 acatacgcat ttaccgcgtg cgcaccaaag agaacggcgt cagtccgccc ccgcaggaat
      661 tcgagcacgg cgtcgtgaat cgtcgccaat ggcaatggtt cacgcattat gaactcgcgg
      721 aatgtgagcg aaccgtcccc gaacatcgat cacctccctg aatgtcagcg tactcacgcg
      781 atacgattcg gacaacccgg cgtgagagcg gcgcgagttg atccatcgca gaaatctcgc
      841 ggccgagatg gacacaggga accgccggct ttctccgcgg agccgagttt cgagttagcc
      901 cgccagctcg cggagccgcc tgaccagctc gaccgctccg tcgggtgtca agttcgtcgt
      961 atcgatctgt ctcagctcga ccaagagtgg atccggcagt gcggcgaaca gactacctgt
     1021 caacgcacgg ccggtcttga ccttcttgcg gatcggccct tcgggcaagc cgggatcggg
     1081 accatgttgc ttctccagaa acgccagaat ctcgctcgcc cgcgccagca ccggcgcggg
     1141 cactccggcc agacgggcga cgtggatccc atagctctgg tcggccccgc caggcacgat
     1201 ctgatagaga aaaacgatct cgccctcttt ttcgcgaacg gccacgttgg cgtttttgag
     1261 gcgagatttg gtcttttcca gctcgacgag ctcgtggtaa tgggtggcga acagggtccg
     1321 acagccgatt gtgtcgtgga tatgttcggt gacggcccac gccagcgaga cgccgtcgaa
     1381 cgtgctggtc ccccggccga tctcgtcgag gattaccagg ctactggcag ttgcattgtt
     1441 gaggatattg gccgtctcgg tcatctcgac catgaatgtg ctctggttgc ggctcaagtc
     1501 gtcggtcgcg ccgactcgcg cgaacagccg gtcgatgata ccgattcgcg cgcgcctggc
     1561 cgggacaaag ctgcccaagt gggcgagaat cgcgattaag gcaacctggc gaatgtaggt
     1621 gcttttgccg gccatgttgg gcccggtgat aagaaggatg gatccgctct tgccgccgag
     1681 ctcgacatta ttgggaacga agtcacctgg ggcgagtcgg gcatcgagca ccggatgccg
     1741 gcccgcctcg atctcgaaga cgggctcagc gaggatctct gggcgacagt atccgtgccg
     1801 ggccgccagc tcggccagcg acgacagaac gtcaagctgg gcgagaaccg accctgcttg
     1861 aattagccgg ggggcctccg ccgacactcg atcgcgcagc gtcgtgaata actcgtattc
     1921 cagctcgtat gcgcggtcct cagcgcggag caccttctcc tcgtattcct tcagctccgg
     1981 cgtgatgtac cgctcggcat ttttgatcgt ctgcttgcgg atgtaatcct cgggaatgtt
     2041 cgcaccccgc gcctgggcct gggcgtgggt aatctcgatg tagtaaccga agaccttgtt
     2101 gaaaccgacc ttcagattcg tgatacccgt gcgccgcacc tgctcggcct gaaacttggc
     2161 gatccacgat ttgccccccc gagcgctgcc gcgcagctcg tccaagccgg agtggtaacc
     2221 gtcgcgcatc agaccccctt ccttgacggc caatggcggg tcatccacca gcgcggactc
     2281 gatctcggcc cggatctcgg ggcagagctc gagggccgcc tcgagctggt tgagccgctt
     2341 cgagaccctc gcggtcagac gagccttgag tttgggcaag atggccaatg ttctggccag
     2401 ggcgacgagg tcgcgcggcg tcgccctgcc ggtgccgacg cgcccagcca gccgctccat
     2461 gtcgtatgac tggcctagca agttcctcag atcgccgcgc aaggccgagt ggttgaacaa
     2521 ctcctcgacc gcctccagcc tctcggcgat caactccgtc gacgtcagcg gagacgtcag
     2581 ccattcactc aagagccgcg cacccatcgg ggtcgccgag caatctatga cgctcagtag
     2641 ggaaccctca cgctttcctt cacgcagggt tcggacgagc tcgaggctgc gacgagtcat
     2701 ctcgtcgagc gcgagcgtgt cggcccgtcg ataagggatc aggcgagtga tatggccgag
     2761 cgcagttttc tgcgtctcgc gcagataggc catgagcgcc ccggccgcct ggacttcggg
     2821 gctgcggtcg tcaattccga agccggccag ggtctttgta tggaactgct caaagagcgt
     2881 tgtgcgcgcc tgctcgggct gaaagtccca agagtggcgg atcgtgaacg ccatccctga
     2941 ttggcttcgc aacaagcgag cccacggggc gtcgttactg agctcggaaa tcaaaacttc
     3001 ggccgggttg agccgtgcga cctcgtcgat cagctcattc cgcaacagcg ccgtcagcga
     3061 aaaccgcccc gtcgacagct cgacccatgc cagtcctagc ttccccccgg ct
//
LOCUS       DATAQL010000957         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4371034, whole genome shotgun sequence.
ACCESSION   DATAQL010000957 DATAQL010000000
VERSION     DATAQL010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4371034"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VHS97_16665"
     CDS             complement(<1..206)
                     /locus_tag="VHS97_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449889.1"
                     /translation="MHPYVALALGLRSRGHEAILATGECYRRKVEALDVGFRAVRPDC
                     AWVADPVIMRRLTHPRRGIIRAVQV"
     gene            complement(277..1290)
                     /locus_tag="VHS97_16670"
     CDS             complement(277..1290)
                     /locus_tag="VHS97_16670"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3449890.1"
                     /translation="MPPHGPSPALFFDTISAFQRTEALRAAIELDLFSLVAAGRQTAT
                     ELANACQAAPRGIRILADNLTIIGFLHKHDDRYELTADAKAFLDRKSPAYLGGAISFM
                     LAPGMKESFQQLTAAVRRGGTAVSEEGTVSHDNPIWVEFARAMAPLMQMPAQLLAGLV
                     GGDSGQPLRVLDVAAGHGLFGITIAAQYRQAHVTALDWPNVLAVAAENARRAGVAERY
                     ALRPGSAFDVDWGGPYDIVLLTNFLHHFDLPTCEQLAAKAYAALAPGGRALTLEFIPE
                     PDRITPPSTASFALVMLATTAHGDAYTFAELQGVFARAGFLRSEFHALPPTTQQAVVS
                     HKG"
     gene            1460..2575
                     /locus_tag="VHS97_16675"
     CDS             1460..2575
                     /locus_tag="VHS97_16675"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX3449891.1"
                     /translation="MSWREAFVTSLGPGYFGGTTPSIWLRVLRENQFDVDWPYWPRAL
                     VTTLGSFQNALLSGWEHLVYARRVQQATVYPPLFILGIFRSGTTLLHNLLVQDDRFAY
                     PNVYQCCYPKTFLTTESTNARVTGFFLPDRRPQDGIALGMRETQEEEFALCSLTGRNF
                     VMGWVFPRRAEQFNRYLTFRDATEEEVAEWKSALTGFIRKLSFKYERPLILKSPGNTA
                     RIRLLLEIFPDARFVHIHRNPYDVFQSIRHTYDKVTPWINLQRPEFDGLDERILRQNR
                     EAYEAFFEERYLIPQDRFHELSFAALEADLVGQVRAIYEGLGLPDFGYVETRLRRYAE
                     SLSGYRKNAFPELSAYERHRIGREWRRCFDEWGYPLD"
     gene            2794..>3269
                     /locus_tag="VHS97_16680"
     CDS             2794..>3269
                     /locus_tag="VHS97_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449892.1"
                     /translation="MRVEIGMRRLWLGPSLAAAAALALGAVWWGLKARTQRQYQAAFL
                     EGKAAAGAGAMEKARRALARAAALRPDPGEAQYLLGAVEKAMGRPDAARAAWLAVPSG
                     SPFALHAAMMLARGALVHDRQAEAEPYLQAALAAPMPTGQEAREVLLNLYKVQNRL"
BASE COUNT          618 a         1045 c         1009 g          597 t
ORIGIN      
        1 acctgcacag cgcggatgat cccccggcgc ggatgcgtca ggcgtcgcat gataacggga
       61 tcagccaccc acgcgcaatc aggtcgcacg gcgcggaagc caacgtccag agcctcgact
      121 ttccgccgat agcattcccc cgttgccagg atcgcctcgt ggcctcgcga ccgcaaaccc
      181 agcgcgagag ccacgtatgg gtgcaagtcg ccgagcgaac cccaggtcgt gatcagaatt
      241 cgaatgggta tccctcgctg cgattcctca ttccattcaa cccttatgcg acaccaccgc
      301 ctgttgcgtg gtcggcggca gagcgtggaa ttcgctgcgg aggaaaccag cccgggcaaa
      361 aaccccttgc aattccgcaa aagtgtaagc gtcgccgtgg gctgtggtgg caagcatcac
      421 cagcgcgaag ctggccgtcg atggcggtgt gatgcgatcg ggctcgggaa tgaattccag
      481 cgtaagggca cggccgcccg gcgcgagcgc cgcgtacgct ttggccgcca actgctcaca
      541 ggtcggcaga tcgaaatgat gcagaaagtt tgtcagtagc acgatgtcgt acgggccgcc
      601 ccaatccacg tcgaaagcac tacctggacg caaagcatac cgttcggcaa cacctgctcg
      661 acgcgcattt tccgctgcaa ccgccagcac attgggccaa tcgagcgcgg tgacgtgagc
      721 ctggcgatac tgagccgcga tcgtaatgcc aaacaacccg tgccccgcgg cgacatcgag
      781 cactcggagc ggttgcccgc tatctccgcc gaccaggccc gcaagcaact gggccggcat
      841 ctgcataagc ggagccattg cgcgagcaaa ctcaacccag atcgggttgt cgtgggatac
      901 tgttccctcc tcggaaactg ccgtgccgcc gcgccgcacg gccgccgtca attgctgaaa
      961 gctctccttc attccgggtg cgagcataaa actaatcgcg ccgcccagat aggccggcga
     1021 cttgcgatcg agaaacgcct tggcgtcggc ggtgagttcg tagcggtcgt cgtgcttgtg
     1081 aaggaagccg atgatcgtga gattgtctgc caatatgcgg atgccccgcg gcgcggcctg
     1141 gcacgcattg gctagctcgg tcgccgtctg gcgaccagca gccactagcg agaacaaatc
     1201 aagttcgatg gccgcccgga gagcttcggt acgctgaaaa gcgctgatcg tgtcgaaaaa
     1261 caacgcgggg gatggtccat ggggaggcat tgcgtctcct gactcatgcg aggaaaggga
     1321 cctgaatcac agcccactgc tcaacattga ttatacgaaa cggggcgaaa cctcgaaaac
     1381 cgtcgagtgt tgctggttgt gccacgttaa gggagtattt gatttggtga caatcggccc
     1441 ttttcgaggc aggtaccgca tgtcctggcg tgaagcgttc gtcacgagtc tcggccccgg
     1501 ctatttcggc gggaccactc cgagcatctg gctaagggtg cttcgcgaga atcagttcga
     1561 tgtcgattgg ccgtactggc ctcgggcgct ggtgaccacc ctcggcagtt tccagaatgc
     1621 tctgctctct ggctgggagc acctggttta cgcccggaga gttcagcaag cgacagttta
     1681 tccgccgcta tttatcctcg gcatctttag aagcggcacc accttgctgc ataacttgct
     1741 tgtccaggac gaccgtttcg cctatcccaa cgtctatcag tgctgctacc ccaagacgtt
     1801 tcttactaca gagtcgacga acgctcgggt cacggggttt ttccttcccg atcgacgccc
     1861 tcaagacggg atcgccctgg gcatgcgcga gacgcaagag gaggagttcg cgctctgctc
     1921 gctgaccggc cgaaacttcg tgatgggctg ggtgttccct cgccgagctg agcagttcaa
     1981 ccgttacctc acgttccgcg acgctaccga ggaagaggtc gcggaatgga aatctgcact
     2041 caccgggttc attcgcaagc tctcattcaa gtacgagcgg ccacttatct tgaagtcacc
     2101 gggcaacacc gctcggattc ggttgctcct ggaaatcttc cccgacgccc ggttcgtcca
     2161 catccaccgg aacccctacg acgtctttca gtcaatccgg cacacttacg acaaagtcac
     2221 cccgtggatc aaccttcagc gtccggaatt cgatggcctc gacgagcgca tcctccggca
     2281 gaaccgtgag gcgtacgagg ctttcttcga ggaacgctac ttgatccccc aagaccgatt
     2341 ccacgagctc agcttcgcgg cactcgaagc cgaccttgtc ggccaagtgc gagctattta
     2401 tgaggggctc gggcttcctg atttcgggta cgtcgagacg cgactgcgac ggtatgccga
     2461 gtcgctctcc ggctacagga agaacgcgtt tcccgagctt tccgcatacg agcgacatcg
     2521 cattggccgg gagtggcggc gttgtttcga cgagtggggc taccctctgg actgaccgct
     2581 tcaccgagac cgagcaggcg ctgactgggg ccctcactgt gaccccggcc ggacaaaacg
     2641 cggtcgacgt caatcagcac ttgattcacc tgttcctgca gcaagcgatg cggccgggcc
     2701 ggggcgagtg gatcgcacag gtgttcacag gaactggtcg tccggccata atggcgagaa
     2761 cggcccgggg gccgagcccc cgggtgcggg cggatgcgag tggagatcgg gatgcgacga
     2821 ctctggctgg gcccgtccct cgccgccgcg gcggccctcg cgctgggggc ggtttggtgg
     2881 gggttgaagg cccgcaccca gcggcagtac caggcggcgt tcctggaggg gaaggccgcg
     2941 gccggcgccg gggcgatgga gaaggcccgg cgcgccctcg cgcgcgctgc ggcacttcgg
     3001 cccgacccgg gggaggcaca atacctcctc ggcgccgtcg agaaggcgat gggccggccc
     3061 gacgccgctc gcgcggcctg gctggcagtc ccctccgggt ccccgttcgc cctccacgcg
     3121 gccatgatgc tcgcccgcgg cgccttggtc cacgaccgtc aggccgaggc cgaaccctat
     3181 ctccaggcgg ccctcgccgc cccgatgccg actggacagg aggcccgcga ggtcctgctg
     3241 aatctttaca aggtccagaa ccgcctgga
//
LOCUS       DATAQL010000958         6371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5136706, whole genome shotgun sequence.
ACCESSION   DATAQL010000958 DATAQL010000000
VERSION     DATAQL010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5136706"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VHS97_16685"
     CDS             complement(<1..878)
                     /locus_tag="VHS97_16685"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3449893.1"
                     /translation="MADSWGSNGASDRLNGPEAAELSRQIAGLAQAGLPLASGLVALG
                     EELPRGRLRRSMNELAATLESGATLDQAVQIQNKRIPPHLRGLVIAGLRTGRLVDILS
                     RFSEYVGVGTELKRRLWLNLAYPVLTVGMALALFFFVCVILVGQFDAIYRDFGIPLPK
                     LTLALIAISRVVNQAWAPLAIVVGALFCGWLAARTLLPVPVRRGLAGRLPLVGPVWRS
                     TSIAEFCHLLALLLESQLPLPEAIRLTGEGVQDASIDASCRLMARDVESGRSLSDAMA
                     ARPLFPQGLPRLLRWG"
     gene            complement(969..2051)
                     /locus_tag="VHS97_16690"
     CDS             complement(969..2051)
                     /locus_tag="VHS97_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3449894.1"
                     /translation="MSAGEMEPRGAGSITIDQLLALNAEIAALVRAGVPLERGLVIAG
                     RDLKGRLGKISTALSQRLSRGESLYQALEGEGQAIPPLYRAVVEAGARSGQLPIALEG
                     LARYVRGYTEARSAIGLALWYPLIVLSLAYVLFVGMVGLIVPRFAGAFGSLELAVPAP
                     LRWMSLVGEYLPYWWPVGPVFLVVLLVAWIRTGAAARFGARSWNWLRLFPWMKSILAN
                     YETANFSELLGLLLKHQVPYGSALILAAESTGNPRLTQGARQLAAAIGRGESTATALG
                     SIDRGTFLPMMRWVLATGEQQGSLVTALHNLADVYRRRAHYQAEKLAVFLPTILMIAI
                     GASATLFYCLALFVPLANMLRQLTAA"
     gene            complement(2146..3387)
                     /locus_tag="VHS97_16695"
     CDS             complement(2146..3387)
                     /locus_tag="VHS97_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX3449895.1"
                     /translation="MISHQLSEAMGRLDATSPRYATDVVELALTEAQAAGASDIHFQP
                     GADGVEVRWRIDGVLQPVAILPARAAPNIVGRLKVLSELLTYRTDIPQEGRIRGMPGA
                     VEMRVSTFPTLFGEKAVVRMFAAQGRFLRLDDLSLPATVRDELDHLLGETSGAIVLSG
                     PAGSGKTTTIYACLRELTARSHGERSLATLEDPIESVVPGVAQAQVNTAAGLTLELGL
                     KSLLRQDPEVIAIGEIRDKNTAEIALQAALSGHLILTTFHAGSACEVIGRFLDMGIEP
                     YVVRSGLRAVVAQRLVRRLCECAVATSRREDFLGLPVQHAKLPRGCELCRGTGYQGRT
                     LLAELLSPETDELAGALLARSDVRQLERAAVAAGMIDRWERAITAVEEGLTSPIEVRR
                     VLGVGAFPDRRGPHISRQDSS"
     gene            complement(3691..>6371)
                     /locus_tag="VHS97_16700"
     CDS             complement(3691..>6371)
                     /locus_tag="VHS97_16700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3449896.1"
                     /translation="IVKGLNTGSPKYCGLEVASGRHLPESWRGSLLTNDFRGNRVCRF
                     VLADDGAGFAAREQPELIKSSHVAFRPIDIKMGPDGAIYIADWYNPIIQHGEVDFRDP
                     RRDHTRGRIWRVTAKGRPLSSRPELAGATEGALLEALKAQEDWTRHQAKRVLKDRGAA
                     MVVPKLASWISDLDSSDPEYDHHRLEALWTYQALDVAEPGLLQALLHSSDARARAAAT
                     RVIPFWKTRLADPQSLLAASVRDAEPRVRLEAVRGLAQIPSLQAAELAVTALDRPVDS
                     FLEYGLWLTVRQLAPIWLPEVLASRFDFGGRPAHLIFALTAVDSPEIVQPLLALLRDG
                     KVPKDHDEAVQNLIARLGKPTDLAVIFDLVVGDQKLPASRRASLLSALVRATRDRKVV
                     PPGDLARVGTLLTSHDETLREAAVRAIGTWKIGSLTKRLIELVGAADTSAGVFAAAIE
                     ALVKDGGVEGRRMVESLAKGGATPDVQARVLAALLESDPKTTAPRLASWLASLAPDQF
                     GAAAIVLARVLERRGAPALLASALAQNGAIFSPDLAKLCIRQVRDSGRDEPGLIAALQ
                     KAGRLKESSLRLSDREMNRLLIDVSRLGDPARGEVVFRRKDLNCLKCHAIAGAGGQVG
                     PGLESIGASAQPDYLVDSLLEPGKAVKENYHALTVATSDGKVYTGVKLRQTGSELVLR
                     DSEGREVAIPTSAVDEQKEAGSLMPVGLTDSLTRPELVDLIRFLSELGKIGPFAAGTD
                     RVLRCWQIPEPSAELNSVADKESVETSLAKRDKLTWRPAFTTVVGVLPLSEWPRTDGR
                     SGAPRLAVASARLEVTSPGPVKLSFNSTDFVRIWIDGNRVLPASDSPRSVIVDLARGS
                     HSIDLAIDLARRRDGIRCVLEDVPGSPARAAAVLGK"
BASE COUNT         1255 a         2163 c         1896 g         1057 t
ORIGIN      
        1 tcgccccacc gcaagagcct agggagcccc tggggaaaga gggggcgcgc cgccatggcg
       61 tccgacagcg aacggcccga ctcgacgtcc ctggccatca accggcatga agcgtcgatg
      121 ctcgcgtcct ggacgccttc accggtcaac cggatggctt cgggcagagg caactggctc
      181 tccaagagca aggcgagtaa atggcagaat tccgcgatcg aagtcgagcg ccagaccgga
      241 cccaccagcg gcagccgccc cgccaggccg cggcgcacgg gcaccggcaa caaggttcgc
      301 gccgccagcc agccgcagaa gagtgcgcct acgacgatcg ccaggggggc ccaggcctga
      361 ttcaccacac gagaaatcgc gatcaaggca agcgtcaact tgggtaaagg aatgccgaag
      421 tcacggtaga tcgcgtcgaa ctgaccgacc aggatgacac agacaaaaaa gaaaagcgca
      481 agcgccatgc cgacagtcaa aacgggataa gccaggttca gccacagcct gcgcttgagc
      541 tcggtcccga cgccgacgta ctcggaaaac cggctcagga tatccaccag tcgacccgtt
      601 ctcagcccgg caattaccag accacgcaga tgggggggaa tgcgcttgtt ctggatctgg
      661 acagcctgat ccagcgtcgc acccgattcg agcgtcgccg ccagctcgtt catcgatcgc
      721 cgcagccggc cgcgaggcaa ctcttcgcca agcgcgacca aaccgctagc cagcggcagg
      781 cccgcctgcg ccagcccggc aatctgacgt gacaactccg cggcctcggg gccgttcagc
      841 ctgtcacttg ccccgtttga tccccacgaa tcggccattc caacccttta gttcggaagg
      901 tgggcttcgc ccaccgttgc tacgttaaac tcgctcgtcc ggagcacgca aattctgcgc
      961 tgactgtttc atgctgcagt taactgccgc agcatgttcg ccagtggcac gaagagcgcc
     1021 agacagtaaa acaaggtcgc gctcgcgccg attgcaatca tcagaatcgt gggcaaaaac
     1081 accgccagct tttccgcctg atagtgggct cgcctgcgat acacatcggc gagattgtgc
     1141 agcgcggtca cgagcgagcc ttgctgctct ccggtcgcca gaacccagcg catcattggc
     1201 agaaacgtcc cgcgatcgat cgaccccagc gccgtcgcgg tagactcgcc tcgccctatg
     1261 gccgctgcca gttgccgcgc gccttgcgtc aaccgcgggt tgccggtcga ctcggccgcc
     1321 agaatcagcg ccgacccata cgggacctgg tgtttgagca agagcccaag cagctcggaa
     1381 aagttggcgg tttcataatt ggccaggatg gacttcatcc agggaaagag ccgcagccag
     1441 ttccatgacc gggctccaaa ccgcgccgcc gcgccggttc gaatccacgc aacgaggagc
     1501 acaaccaaga ataccggccc aaccggccac cagtacggca ggtattcgcc aaccagtgac
     1561 atccatcgca acggagccgg cactgcaagt tccagcgagc cgaaagcgcc cgcaaatctc
     1621 ggcacgatca gccccaccat gccgacgaac aagacgtagg cgagcgacaa gacgatgagc
     1681 ggataccaaa gtgccagccc aatggcggat cgggcttcgg tgtagccacg gacatagcgc
     1741 gcgaggccct cgagcgcgat cggcaactgc cccgaccgtg cccccgcctc cacgaccgcg
     1801 cggtataggg gcgggatcgc ctgaccttct ccctcgagcg cctggtacag gctctctccc
     1861 cggctcagcc gctgcgacag ggccgtcgaa atcttgccga gccgcccctt gaggtcgcgg
     1921 ccggcgatca cgagcccccg ctcgagcggg actcccgcac ggaccaacgc cgcgatttcc
     1981 gcattgagag ccagcaattg atcgattgta atcgaaccgg cgccccgggg ctccatctct
     2041 ccagctgaca ttacatacct ttccgactcg cccgacccgc cggcgccacc gcaacagcct
     2101 acgacacttg tatcattttt atctcaccac aagtgtcaga ggacgtcaag acgagtcctg
     2161 gcgggaaatg tgcggccctc tgcggtcggg aaacgcgccg acgcccaaaa cgcggcgcac
     2221 ttcgatcggc gacgtaagcc cctcttcgac ggccgtgatg gcgcgttccc agcgatcgat
     2281 catgccggcg gcgacggccg cgcgctcgag ctggcgcacg tcgcttcggg ccaataaggc
     2341 cccggccagc tcgtcggtct cgggcgagag cagttcggcg agaagagtcc gcccctggta
     2401 gccagtcccc cggcaaagct cacaaccgcg tgggagcttg gcgtgctgca caggcaaacc
     2461 caggaaatct tctcgtcggc tcgttgcgac cgcgcactcg cacaggcggc gaacaaggcg
     2521 ctgcgcgacg accgcgcgca gtccactgcg aaccacgtag ggctcgattc ccatgtcaag
     2581 aaatcggccg atcacctcac acgcgctgcc ggcgtgaaat gtggtgagta tcaaatgacc
     2641 gctcaaagca gcctggagcg caatctcggc ggtgtttttg tctcgaatct cgccgatcgc
     2701 gatcacctcg gggtcttgac gcaagagcga tttgagtccc agctcgagtg tcagcccggc
     2761 tgcggtgttg acctgggcct gcgcgacgcc tggcactacg gactcgatcg gatcttccag
     2821 cgtcgctaag ctgcgctccc catgtgaccg ggccgttagc tcgcgcaggc aggcatagat
     2881 cgtggtggtt ttgccgctgc ccgcggggcc cgagagaaca atcgcgccgg acgtctcacc
     2941 cagcaagtga tcaagttcat cgcggaccgt ggccggcaag ctcaggtcgt caaggcggag
     3001 aaaccgccct tgtgccgcga acatccgcac gaccgctttc tcgccgaaca aggttggaaa
     3061 ggtgctgacc ctcatttcca ccgccccagg catgccgcga atccgtcctt cctggggaat
     3121 gtcggtgcga taggtcaata gttccgacaa gactttgagt ctgcccacga tgttgggcgc
     3181 ggccctagcc ggcaaaatcg cgaccggctg caatacgccg tcgatccgcc accgcacctc
     3241 cacgccgtcg gcacctggct ggaaatgaat atcactggca ccggcagcct gggcctcggt
     3301 caaggcgagc tcgaccacgt cagtcgcgta acgcggacta gtggcatcga gcctccccat
     3361 cgcctcggat agctggtgag atatcatcga tatctaaccc cgccctcctt cgcagcacgt
     3421 ctcgcaaata gtgtggcatt tcgagcagac cagcttggcc ttttgctcga cgagaattcc
     3481 tccacagacg gggcaagttg gccggtaggg tggctgctgg tccccgcctt tttcttcttc
     3541 gaccacactg cgctccattc gtggtttcat cgtcaagcac cgtgcggaaa gggatgagga
     3601 tatgctggtc ccccacgtcg accttataat gatcttaagc cggaacgatg ctggacagca
     3661 agcgcactaa cgcgactcgg cccgggccac tcacttacca agcactgccg cggcgcgcgc
     3721 gggggaaccc ggcacgtctt caagaacgca gcgaattccg tcgcggcgtc gcgccagatc
     3781 gatcgccaag tcgatcgagt gcgagccgcg cgcgagatcc acaatcactg accgcggcga
     3841 gtcgctcgcc ggcaagacgc ggttgccgtc gatccagatc ctcacaaagt ccgtcgaatt
     3901 gaacgacagc ttcactggac cgggagacgt gacctccagt ctcgcgctcg ccacagcaag
     3961 cctaggcgcc cccgaccgcc cgtccgttct gggccactcc gagagcggca gaactcccac
     4021 caccgttgtg aaggccggcc gccaggtgag cttatcccgc ttcgccagcg acgtctccac
     4081 actttcttta tcggcaacgg aattcagttc cgcggacggt tccgggattt gccagcaccg
     4141 cagaacacgg tctgtacccg ctgcaaaagg cccgatcttg ccgagctcgg agagaaaccg
     4201 gatcagatcg accagttcgg ggcgagtcag ggaatcggtc aatcccaccg gcatgagcga
     4261 accggcctcc ttttgctcat cgaccgcgct cgttgggatc gcgacctcgc ggccctcgct
     4321 gtcgcgcaag accagctcgg aaccggtctg gcggagcttg acgccggtgt agaccttgcc
     4381 gtcgctggtc gccaccgtca gcgcgtggta gttttccttg actgccttac cgggctcgag
     4441 caaagaatcg acaaggtagt cgggctgagc actggcgccg atgctttcta gcccagggcc
     4501 aacttgcccg cccgccccgg cgatggcgtg acatttcaga cagttcaggt ccttgcggcg
     4561 aaagaccact tcaccccgcg cagggtcccc cagccgtgac acgtcgatta aaagccgatt
     4621 catctcccga tccgaaagcc ggagagagct ctccttgagc cgtcctgctt tctgcaaggc
     4681 ggcgatcaat ccgggctcgt cgcggcccga atcgcggacc tggcgaatgc acagcttggc
     4741 cagatctgga gaaaaaatcg ccccattttg agcgagggcg gaggccaaaa gcgctggtgc
     4801 cccgcgccgc tcgagaacgc gcgcgagcac aatcgccgcg gcaccgaact ggtcgggcgc
     4861 gaggctggcg agccacgagg ccagccgcgg cgccgtcgtc ttggggtcag actccagaag
     4921 ggcggccaat acgcgagcct ggacatcagg cgtcgcacct ccttttgcca gactctcaac
     4981 catccgacgc ccctcgacac caccgtcctt gaccagtgcc tcgatcgccg ccgcgaaaac
     5041 gccggccgac gtatccgccg cccccaccag ctcgatcagc cgtttggtga gagacccgat
     5101 cttccaggtc ccgattgctc ggacggcggc ttcacgcagt gtttcgtcgt gactggtcaa
     5161 aagcgtgcca acccgagcca ggtcgcccgg tggaacgact ttgcgatctc gcgtggcgcg
     5221 gacgagcgcg ctcaaaagcg aagcgcggcg cgacgccggt agtttttggt cgccgactac
     5281 gagatcgaat atcacggcaa gatccgtcgg ctttcccagg cgggcaatca ggttctggac
     5341 ggcttcgtca tgatctttgg gcacttttcc gtctctcagc agtgccagca gcggctgaac
     5401 aatttccggg gaatccacgg ccgtgagggc aaaaatcagg tgcgccggcc ggccgccgaa
     5461 atcgaaccga gaagccagga cctcgggcag ccagatcggg gcgagctggc ggaccgtcag
     5521 ccacaaccca tattcgagga acgagtcgac cggccgatcg agcgccgtca cagccaactc
     5581 cgccgcttgg agactgggta tctgcgctaa cccgcggacg gcctcgagcc gcactcgggg
     5641 ctccgcatcg cgcacgcttg cggccagaag cgactgtgga tccgccagcc gcgttttcca
     5701 gaacggtatt acgcgtgtcg cggccgcgcg cgctcgagcg tcacttgaat gaagtagagc
     5761 ttgcagcaaa cctggctccg caacgtcaag ggcctgatac gtccagagcg cctcgagacg
     5821 atggtggtcg tattcagggt cgctggagtc gagatccgag atccagctcg ccagcttggg
     5881 aacgaccatg gcggcgccgc gatccttcaa gactcgcttc gcctggtgcc tggtccagtc
     5941 ttcctgcgcc ttgagcgctt ccagcagcgc gccctccgtc gcgcccgcca gctcgggccg
     6001 ggacgagagc ggccgcccct tggccgtcac tcgccagatg cgcccacgcg tgtggtcgcg
     6061 cctcggatcg cgaaaatcca cctcgccgtg ctggatgatc gggttgtacc agtcggcgat
     6121 gtaaatcgcg ccgtcaggtc ccatcttgat gtcgatcggg cggaaggcaa catgagagct
     6181 cttgatcagc tcgggctgct cgcgcgcggc gaagcccgcg ccgtcgtcgg cgagcacgaa
     6241 ccggcagacc cggttgccgc gaaagtcgtt ggtcaaaagg cttccccgcc acgactcggg
     6301 taaatgccgc ccgctggcga cctccagccc gcaatacttg ggactccccg tattaagccc
     6361 ctttacgatc c
//
LOCUS       DATAQL010000959         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2083301, whole genome shotgun sequence.
ACCESSION   DATAQL010000959 DATAQL010000000
VERSION     DATAQL010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083301"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VHS97_16705"
     CDS             <1..1076
                     /locus_tag="VHS97_16705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3449897.1"
                     /translation="SKREMSMANEAKLKQLIGQLLSDLGGASSVAMVRIGGALGLYKA
                     LHARGAMTSRELAAAVGANERYLREWLANQAASNYLSYDSVTQKFALPEEQAMIFAAE
                     DSPVYMLGGFDLMAAMLDNQPKVEAAFRTGGGVAWGEQAGCMFCAVARFFRPGYHNHL
                     IGSWLPALDGVVEKLEYGAKVADVGCGHGWSTVTMAKAFPNSQFIGYDFHLGSIEAAR
                     KHAAVHGVTDNVIFEVGSAKDFAATGLDLVTFFDCLHDMGDPAGAAAHVCRSLKKDGS
                     WMIVEPMAADSLEENLNPVSRLYYAASTMICIPTSLSQDVGTALGAQAGEAKLREVIT
                     AGGFASVRRAAETPFNMILEARH"
     gene            complement(1623..>2095)
                     /locus_tag="VHS97_16710"
     CDS             complement(1623..>2095)
                     /locus_tag="VHS97_16710"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449898.1"
                     /translation="PTLLHLADRIGTRLRAKSRPGRTVTVRVRFANMVSATRSVTLDA
                     PISATVILAEIAEELVRVVLADRPDEKTISLLAISVSHLEEHWDVQLELPLGLKDEAR
                     RPGTKKCMARWAADCAVDRIRDRFGWDAIGYASVALGIYRSVPDEFRKLAEKDL"
BASE COUNT          415 a          615 c          661 g          404 t
ORIGIN      
        1 tctcgaaaag ggagatgagc atggccaatg aagcgaaact caagcaactg atcggtcagt
       61 tgctgtcgga cctcggcggc gcgtccagcg tcgcgatggt tcggatcggc ggcgcgctcg
      121 ggctttacaa ggccctccat gccagggggg ctatgacgag tcgggagctt gccgcagcgg
      181 tcggtgccaa cgagcgctac ttgcgcgagt ggctcgccaa ccaggccgct tcgaactatc
      241 tctcctacga ttcggtgacg caaaaattcg cgcttccgga agaacaggcg atgatcttcg
      301 ccgcggaaga cagcccggtt tatatgctgg gcggcttcga cctgatggcc gcgatgctcg
      361 acaaccagcc caaagtcgag gcggcattcc gcaccggcgg cggcgttgcc tggggcgagc
      421 aggccggctg tatgttttgc gcggtggcgc gcttcttccg acccggctac cacaaccacc
      481 tgattggctc atggcttcct gccctcgacg gcgtcgtcga gaagctggag tacggcgcca
      541 aggtggctga tgtcggctgc ggtcacggct ggtcgacggt gacgatggcc aaggctttcc
      601 caaactcgca atttatcggc tacgacttcc acctgggttc gatcgaggcg gcgcgcaagc
      661 acgccgctgt gcatggcgtc accgacaacg tgatcttcga ggtcggctcg gcaaaggatt
      721 ttgccgctac aggattggac ctcgtgacct tcttcgattg tctgcacgat atgggcgacc
      781 cggctggcgc cgcagctcat gtttgccgct cactgaagaa ggacgggtcg tggatgatcg
      841 tcgaaccaat ggcagccgac agtctcgaag agaacctaaa ccctgttagc cggctttact
      901 atgccgcttc gacaatgatc tgcatcccta cttcgttgtc gcaggatgtt ggaaccgcac
      961 tcggcgctca ggcgggcgaa gcaaagctac gggaggtcat cactgcgggc ggcttcgcct
     1021 cggtcaggcg ggcagcggaa acgccgttca atatgattct agaggcgcgg cactgacaca
     1081 ctgcgtccaa catacatgtc tgtccacact tgcgatgaat gcacaaattc gagttccagt
     1141 gtttgccaca aggaatgacc gctcagaaag gggagtcggc agagggcata tttgcacgag
     1201 gatgcgaaag tcggcttgtg gcacgaagct gcagtgcgat cagcgcatag ccagttccgc
     1261 cgtcgaagga aaagcacctg aggcggcagg acgattctgc tctgccgtcc ctgaccaaag
     1321 cagaaatttc gacgttctat gccaccccgg aagcggctgc acaggcataa gcctgatcga
     1381 aacccgcaga gatgagcttc gatgaaccac agctgtttgt ccgtcgccca cgagatttcg
     1441 gtggacacgt cggctgtgcc cgcaccgccc cccagcggcg gtcgaatttg ggttggcgcg
     1501 ccggccttac cgaactgggc cgcctgcctc acggcacgga gatggcagat ggggtcggta
     1561 aagtgtggct gggccgagac cgactccaca acacctgtgt tttttcccgt taccgatgat
     1621 ggttacaggt ccttttcggc gagtttgcga aactcgtcgg gaacagagcg gtaaatccct
     1681 agcgccaccg acgcatatcc gatcgcgtcc catccgaagc gatcgcggat cctatcgaca
     1741 gcgcagtcgg ccgcccaacg cgccatgcat ttcttggtgc cgggacggcg cgcctcatct
     1801 ttaagtccga gtgggagttc cagctgcaca tcccagtgct cctcgagatg cgacacggag
     1861 atcgccagaa gcgagatggt cttctcgtca gggcgatcgg cgaggaccac gcgcaccagc
     1921 tcctcggcga tttcggcaag gatcacggtt gcggagattg gcgcgtcgag cgttaccgag
     1981 cgagtggctg agaccatgtt ggcgaagcga acgcggaccg tcacggttcg accaggcctg
     2041 gacttggccc ggaggcgggt gccgattctg tccgcaagat gaaggagggt gggtc
//
LOCUS       DATAQL010000960         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1779287, whole genome shotgun sequence.
ACCESSION   DATAQL010000960 DATAQL010000000
VERSION     DATAQL010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779287"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2976)
                     /locus_tag="VHS97_16715"
     CDS             complement(<1..>2976)
                     /locus_tag="VHS97_16715"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3449899.1"
                     /translation="AGGMGVVYEAYDQERGELVALKTMRRVDPVALVRFKHEFRSLSD
                     ITHPNLVNLYELFAVDDRWFFTMELVEGCDFVTYVRNQVDPLAIRAGALTVTDGDQRG
                     VLAPPAQDQESKPGRSFDEMRLRLSLTQLAEGVNALHQSGKLHRDIKPPNVLVNMEGR
                     VVLLDFGLTADLESLARQQAVDRQIVGTVGHMSPEQAAGRSVTAASDWYSVGVMLYEA
                     MTGQLPFAGSPDEIFIAKQTSPPQSPDAIVPGLPEDLVSLSLALLDRNPTKRPTGRDV
                     ITRLRGVVAGVADVPEINRSLPLIGRSRHRQVLEGLFASLSHGATEAVFVFGRTGTGK
                     TTLVRSFLDELIERDDAVVLSGRCYERESVPYKALDSLIDALARHLKGLTARELASLL
                     PPNVAYLARLFPVLQSVGAVALARRASTEMPDRQELRRRAFAALRELLKRIGDQRPLI
                     LAIDDLQWGDVDSAILLSDLVCSPQAPVLLFIGCFRSEDLDRSPFLNEIKKSIAGVHH
                     PLEHRELAVEALTQSEARELALALLGRDDAVSRAQAHMVGRESRGNPLFIDELVKHIQ
                     SVEPTDNWEEIGQLDLDEVLWSRIQRQPEDARRLLGVVAVSGRPISQALAFHASELGV
                     GSRVALASLRSARLIRRVGQTQHDDVEIYHDRIRETVVAHLSADALRWHHERLALVLS
                     ASGPIDAEVLAGHLHGAGDHERACEYYARGADQASAALAFDHAARLYRIALELHQGAG
                     AQARILSKKLGDALANAGRGHDAAQAYLQAAESATAAETLELKRLASTQLLISGHVEE
                     GLTLLRTLLGPLGMNMPATAGRAQLSLRWHRALLGLRGLKFRIRDESQISAMDLTRID
                     LCWSAVAGLSMIEPIRGADFQTRGLLLALKAGEPARVARALTMEAGHRSTIGTATSRI
                     AKFLNAAEDIAQRLDSPYLHGMVSLARGVESVMLGRWKAAQTALDQAEQIFRNRCTGV
                     TWERDTVHNFVLWALMQLGE"
BASE COUNT          563 a          943 c          938 g          532 t
ORIGIN      
        1 ttcgccgagc tgcatgagtg cccacaatac aaagttatga accgtgtcgc gttcccaggt
       61 gacccctgtg cagcggttgc ggaagatctg ttcagcctgg tcgagcgcgg tctgggcggc
      121 cttccaccgc cccagcatga cggattcgac gccgcgcgcc agggagacca ttccatgaag
      181 atagggtgaa tcgaggcgct gcgcgatgtc ttcggcggcg ttcaagaatt tggcgatgcg
      241 cgaggtagcg gtcccgatgg tggaccgatg gcccgcctcc atggtcagcg cgcgcgccac
      301 gcgtgcaggt tcgccggcct tcagcgccag caagagcccg cgggtctgaa agtcggcgcc
      361 gcggatcggt tcgatcatcg ataggcccgc aaccgccgac cagcagaggt cgatccttgt
      421 caagtccatc gcggagatct ggctctcgtc tcggatccgg aacttgaggc cgcgcaatcc
      481 cagcagcgcg cggtgccagc gaagggatag ttgggcccgc cccgcggtgg cgggcatgtt
      541 catgcccagc ggtccgagca acgtgcgcag aagggtcagc ccttcttcga cgtgaccgct
      601 gatcaagagc tgggttgaag ccaggcgctt gagctccaac gtttctgcgg cggtggccga
      661 ctcggccgct tggagatacg cttgcgccgc atcgtgcccg cggccggcgt tggccagcgc
      721 gtctcccagc tttttcgaga ggatgcgcgc ctgggcgcca gccccttggt ggagctccag
      781 cgcgatccga tagagccgcg ccgcatggtc aaaggcaaga gccgccgagg cctggtcggc
      841 cccgcgcgca tagtattcgc aggcgcgctc atggtcgccc gcaccatgca gatggcctgc
      901 gagcacctcc gcatcgatcg gtcccgaagc cgacagcacc agcgcgagcc gttcgtgatg
      961 ccatcgcagt gcatcggccg acaggtgcgc caccaccgtc tcccggattc ggtcgtggta
     1021 gatttcaacg tcatcatgtt gggtctggcc aacgcggcgg atcagacgcg cggagcggag
     1081 tgaagctaag gccacacggc tcccgactcc cagctcgctg gcatggaacg cgagagcctg
     1141 gctgatcggc cggccggaaa ccgcgacgac tcccagcagc cggcgcgcat cttcgggctg
     1201 acgctgaatg cgcgaccaga gaacctcgtc gaggtcaagc tggccgatct cctcccaatt
     1261 atcggtaggt tccacgctct ggatatgctt gacgagctca tcgataaaaa ggggattgcc
     1321 ccgggattct ctgccgacca tatgagcctg ggctcgcgag accgcgtcgt cgcgacccaa
     1381 gagcgccaaa gccagctcgc gtgcctccga ctgcgtgagg gcctcgaccg ccagctcgcg
     1441 atgttcgagc ggatggtgca cgccggcgat ggacttcttg atctcgttca agaatggact
     1501 gcgatcgaga tcctccgacc gaaagcaacc gatgaagagc aacaccggcg cctggggcga
     1561 gcagaccagg tcggataaca agatcgcgct atcaacgtct ccccactgca ggtcgtcgat
     1621 tgccaagatc aatggcctct ggtcgccgat ccgctttagc agctcgcgca aggcagcgaa
     1681 ggcacgccgt ctgagttctt gccggtcggg catctcggtc gacgcacggc gcgccagtgc
     1741 aacggctccg acgctctgca agacgggaaa aagtcgggcc agatacgcca cattcggggg
     1801 gagcaggctg gccaattcgc gagccgtcag ccctttcaga tgccgcgcca gggcatcgat
     1861 caggctgtcg agggctttgt atggcaccga ttctcgctcg taacaccgcc cagatagaac
     1921 caccgcgtcg tcgcgctcga tcaactcgtc gaggaacgac cgaaccaatg tggtcttgcc
     1981 cgtacccgtg cggccgaaaa caaacaccgc ctcggtcgcg ccgtggctta gcgaggcaaa
     2041 tagcccttcg agaacttggc gatgcctcga ccgtccgatc aagggaagcg agcgattgat
     2101 ttccggtacg tcagccacgc ctgccacaac accgcgcaat cgtgtaatca catcgcggcc
     2161 agttggtctt ttcgtgggat ttcggtctag gagagcgagg gacaggctca ccagatcctc
     2221 cggcaaaccc ggtacgattg catcgggcga ttggggagga ctggtctgtt tcgcaatgaa
     2281 tatctcgtcg ggtgagcccg cgaacgggag ctgtccggtc attgcctcgt aaagcatgac
     2341 cccgacgctg taccagtcgc tggcggcggt gaccgaccgg ccggccgctt gctccggcga
     2401 catgtggccg acggtcccaa cgatctggcg gtcgaccgcc tgctgtctag ccagactctc
     2461 aaggtcggcc gtcagcccaa aatcgaggag aacgacgcgg ccctccatgt tcaccaggac
     2521 gttgggcggc ttgatgtcac gatgcagctt gccagactgg tggagagcat tcacgccctc
     2581 ggcaagctga gtcagtgata aacgaagccg catttcgtca aatgagcgac cgggcttcga
     2641 ctcctgatct tgtgctggcg gtgcaagtac cccccgttgg tcgccgtctg tcacggtgag
     2701 cgcacccgct cgaattgcaa gcgggtcgac ctggttcctc acgtaggtga caaagtcgca
     2761 gccttcgacc agctccatgg taaagaacca gcggtcgtcg accgcgaaca gctcgtacaa
     2821 attgacaagg ttgggatgtg tgatatcgct cagggaccga aattcgtgct tgaatcggac
     2881 cagggcgacg gggtcgaccc gacgcatggt tttgagtgcg accagctcgc cgcgctcctg
     2941 gtcgtaggcc tcgtatacta cccccattcc gccggc
//
LOCUS       DATAQL010000961         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1779294, whole genome shotgun sequence.
ACCESSION   DATAQL010000961 DATAQL010000000
VERSION     DATAQL010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779294"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VHS97_16720"
     CDS             <1..1368
                     /locus_tag="VHS97_16720"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEX3449900.1"
                     /translation="ERGIAGIPTAWMATWGSGKPVIALGSDIDCIPKASQKPGIARHE
                     PLCDGAPGHGEGHNSGQPLNITAAIAVKRLMERSKIPGTIKLWPGVGEELLGTKAYLV
                     RAGCFKDVDVVLFTHVGDNLAVSWGTGDATGMVSVEYTFQGESAHAGSAPWRGRSALD
                     AVELMNIGWNFRREHLRPQQRSHYVITGGGDQPNVVPSSASVWYYFRELDYPHIKQMR
                     EIGDTIAQGAAMMTNTTVTSRVLGSAWPLHFNKPVAEAMYQNIKQIGLPQWSEADQVL
                     AKAVQRELKIPERGLDSKLKPLEGPVSDDRRTKGGSDDIGDVSWNVPTVTLRYPSNLP
                     NLPGHHWSNAITMATPIAHKGVTAGAKVLATTMVDLLLQPALVREAWDYFRDVQTKDT
                     KYQPLISAHDQPAVWSNKKTMDEYRPQMRKFYYNPAMYDTYLDQLGISYQGGGGGTHS
                     SRNGR"
     gene            1531..2745
                     /locus_tag="VHS97_16725"
     CDS             1531..2745
                     /locus_tag="VHS97_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX3449901.1"
                     /translation="MRGRYPCGGMHRREFLGATAVPFLVQSALSAQQPKGGANSPASG
                     GPLGIPGPYPGRVIEVRNPALDRGTRKDRAAMRATLNRGLVLLTGADHPVEAWRTFVQ
                     PGESVGIKVVPNGYPGAHTSPELILEVITGLRAAGIAFKDMVVFDRYGLEFREARYQE
                     ILPDGVAWGGLTPVAWDPGQLAIDFDGKDPIAGYDRDEYVKLTLVGRGQDPKDDRCFR
                     SHLGKIVTKRLDKIIGLPCIKDHHAAGVTGALKNMSHGLVNNVFRSHSSPQSCAMVAF
                     IPAVVSHPIIRKKCVLHIMDGTRCVWEGGPFGRHPEWLWEHNALLFATDPVAMDHVEW
                     DILDAQRKAKGVPGVGGVGRLAADPFKQEGYDIRQPQYVAMAGQAGLGLFDYKSPSGR
                     RFSIDHRVENLS"
     gene            2887..>3664
                     /locus_tag="VHS97_16730"
     CDS             2887..>3664
                     /locus_tag="VHS97_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449902.1"
                     /translation="MGSVHAHGRSAKRKRRRLALVLSLAALGVGLAGSARLWVRGAPA
                     DRQHLDLLFDAWNEFNAKRYDRATAILDHRTALVEPIALDWMLRAQIAESQGGLDQAL
                     EYLKHINDSDPIGARAWLKAGQIEKARYNARSAEAAFRRSVELDHKQTQAYRELVYLY
                     ALERRKAECDDLFRAFARLVTFDDVLAFAWAQNDCEIWDPNEAIPVLAAIIANDTADR
                     FSRLALATNYRLMKAFEQAVATLEPLPDSDPDARAIRVQIA"
BASE COUNT          712 a         1136 c         1170 g          646 t
ORIGIN      
        1 gagcgcggca tcgcgggcat tccgaccgcg tggatggcga cctggggatc gggcaagccg
       61 gtgatcgcgc tcggctccga catcgactgc atccccaagg cttctcagaa accgggcatc
      121 gctcgtcacg agccgctctg cgatggcgcg ccaggacacg gcgagggtca taattcgggc
      181 cagcccctca acatcacggc ggcgattgcg gtcaaacggc tgatggagcg aagcaaaatt
      241 ccaggcacaa tcaagctctg gccgggagtc ggtgaagagc tgctcgggac caaggcgtac
      301 ctggtgcgtg ctggctgttt caaggatgtg gatgtcgttc tctttacgca cgtcggcgac
      361 aacctcgctg tctcgtgggg aacgggcgac gcgactggca tggtgtctgt cgagtacacc
      421 ttccaaggcg agagcgcaca cgcgggttcc gccccgtggc gaggccgaag cgccttggat
      481 gcggtggagt taatgaacat cggctggaac ttccggcgcg agcacctgcg cccccagcag
      541 cgctcgcatt atgtcatcac cggcggtggc gatcagccga acgtggtgcc gtcgtcggcg
      601 agcgtctggt actacttccg cgagctggat tatccgcaca tcaagcagat gcgggagatc
      661 ggtgatacca tcgcgcaagg cgcggccatg atgaccaata caacggtcac gtcgcgcgtg
      721 ctgggttcag cttggcctct gcatttcaac aagccggtag ccgaggcgat gtaccagaac
      781 atcaagcaga tcggcctgcc gcaatggagc gaagccgacc aagtcctggc gaaggccgtg
      841 cagagggagt tgaagatccc cgagcgcggc ctggattcga agctcaaacc gctcgaaggg
      901 cccgtgagcg acgaccggag gaccaaaggg ggttccgatg acatcggcga cgtctcatgg
      961 aatgtcccga ccgtcaccct gcgatatccg tcgaatctcc ccaatctgcc gggccatcat
     1021 tggtcgaacg cgatcacgat ggcgacgccc atcgcccata agggcgtgac ggcgggtgcg
     1081 aaagtgctgg cgacgacgat ggtcgatctg ctcttgcaac cggcgctcgt acgagaggcc
     1141 tgggattatt tccgggatgt tcagaccaag gacaccaagt accagccgct catctcggcc
     1201 cacgatcagc cggcggtctg gtccaacaag aagaccatgg acgagtaccg cccgcaaatg
     1261 cggaagttct attataatcc ggcgatgtat gatacctatc tcgatcagtt gggtatcagc
     1321 taccagggcg gtgggggagg gacccactcg tcgcgaaacg gaagataggc gtcatccaca
     1381 cacattcgca cagtgcccta ttgcacggca tctgaaccgc cggacaatga agcgtgtttg
     1441 ccgggattgg ctcagtgcgg cacttaactc ggcccgccaa gagcgcgggc tgcacttcct
     1501 gtttcgtgga ccaatcgaag gagccgaaac atgcgtgggc gatatccctg tggcggcatg
     1561 catcgtcgcg agtttttggg tgcaaccgcc gttcccttct tggtgcaatc ggccctcagt
     1621 gcccagcagc ccaagggcgg ggccaattcg cccgcctctg gcgggccgct gggcattccc
     1681 ggcccctacc cagggcgcgt gattgaggtt cgcaacccgg ctctcgaccg cgggaccagg
     1741 aaggaccgcg cggcaatgcg ggcgactctc aaccggggtc tcgtcctgct cacgggtgcg
     1801 gaccatcctg tcgaggcctg gcggaccttc gtgcagccgg gtgagtccgt cggaatcaag
     1861 gtcgtgccca acggttatcc cggggcccac acctcgcccg agctgatcct ggaggtaatc
     1921 accggcctcc gagcggcggg gatcgcgttc aaagacatgg ttgtcttcga ccgctatgga
     1981 ctcgagttcc gtgaggcgcg ctatcaggaa atcctccccg acggcgtcgc ctgggggggc
     2041 ctgaccccgg tcgcctggga ccccggccaa ttggcgatcg atttcgacgg taaggacccg
     2101 atcgccggct atgaccgcga cgaatacgtt aagttgacgc tcgtcggccg gggacaggac
     2161 ccgaaggacg acaggtgctt tcgatcgcac ctcggtaaga tcgtgaccaa acggctcgac
     2221 aagatcatcg gcctgccgtg catcaaagac caccacgccg ctggcgtgac cggcgccctg
     2281 aagaacatga gccacgggct agtcaacaac gtcttccgct cgcactcaag cccccagagc
     2341 tgtgcgatgg tcgccttcat ccccgcggtg gtcagccacc cgatcattcg caagaagtgc
     2401 gtcttgcaca tcatggacgg cacccgctgc gtctgggagg gagggccgtt tggcagacat
     2461 cccgagtggc tgtgggagca caacgcgctg ttattcgcga ccgacccggt ggcgatggac
     2521 cacgtcgagt gggacatcct cgacgcccaa agaaaggcga agggagtgcc gggtgtcggt
     2581 ggcgtcggcc gtctcgcggc cgatccgttc aagcaggaag ggtacgacat ccgccagcca
     2641 cagtacgtcg cgatggccgg ccaggccggc ctgggtctct ttgactacaa gtcgccaagc
     2701 ggccggcggt tttcgattga tcaccgcgta gaaaacctct cttgaggcga gcgtctacgc
     2761 accatccttt cctcccgtcc aagaaccacc acacgcccgg gggcctgccg gacgtgccgg
     2821 atcttgtctg gctgcctcat ttgtcgagac gacaacgatc cgataaagtg gggacgggag
     2881 gtaggcatgg gatcggttca cgcccacggg cgatcggcaa agcggaagcg acggagactg
     2941 gctcttgtct tgtcgctggc tgcgctcggg gttggattgg cggggtccgc ccggctgtgg
     3001 gtgcggggcg cgccagcgga tcgccagcac ctcgacttgc tgtttgacgc ctggaacgag
     3061 ttcaacgcca agcgttacga ccgcgccacc gcaatcttgg accaccggac ggcactagtc
     3121 gagccaatcg ccctggactg gatgttgcgg gcgcagatcg ccgaatcgca aggtgggctg
     3181 gaccaagccc tggaatacct aaagcatatc aatgactcag atccgatcgg agcgcgggcc
     3241 tggctcaagg cgggccaaat cgaaaaagcg cggtacaatg cgcgaagtgc cgaagcggcg
     3301 ttccgccgct cagtcgagct cgatcacaag caaacccagg cgtatcgcga gctggtctat
     3361 ctctacgccc ttgagcgccg caaggcggag tgcgatgacc tgtttcgggc cttcgcgcgg
     3421 ctcgtgacct tcgacgacgt cttggccttt gcctgggcac agaacgattg cgagatctgg
     3481 gatccaaacg aggcgattcc tgttctggca gccatcatcg ccaatgacac cgccgaccgc
     3541 ttctcgcggc tggcgctggc gaccaactac cgactgatga aggcgtttga gcaggccgtg
     3601 gccacgctcg aacccctgcc ggattcagac cccgatgctc gcgcgattcg tgtccagatc
     3661 gcca
//
LOCUS       DATAQL010000962         4138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4525688, whole genome shotgun sequence.
ACCESSION   DATAQL010000962 DATAQL010000000
VERSION     DATAQL010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4525688"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1485)
                     /locus_tag="VHS97_16735"
     CDS             complement(151..1485)
                     /locus_tag="VHS97_16735"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3449903.1"
                     /translation="MHNWNRREFLAASALAVAQAEAPKKAGDQRKAALSAGEKPNSAP
                     VKVAPADKIVLAFIGVGGMGTGLVNKFREFPDVEIAAVCDVYEPHLRRAQRAAGERTE
                     TYGDFRRVLDRKDLDAVVIATPDHWHGIPTILACQAGKDVYCEKPLAHRIQEGRAMVQ
                     AAEKYKRVTQMGNLIHAGENYRRVVEIVRSGVLGKITKTRVWLAADRSGMGRPQDGSP
                     PPGCDYDFWLGPAPSRPFNANRFTFNWRYFWDYGGGILTDFCCYIVDLVHWAMEVDAP
                     HTISATGGRYALADNAEVPDTLDVTYEYQKGDQKFLMVWSHTDASTHGLDNKGLGIMF
                     QGTDATLVADYGTYRIFNEKGKSVEIPAPTIPRSVGHHREWLDAIKSRGRCSCHFEYG
                     HRLSTVGHLGNIALWTGEKLHWDARNEVTNHPEANRHLAREHYRAPWTLPKV"
     gene            1827..3716
                     /locus_tag="VHS97_16740"
     CDS             1827..3716
                     /locus_tag="VHS97_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3449904.1"
                     /translation="MPRFLRMTLVTGLTCTMPVLALYAQQPPPSAPASQPAAKTATKS
                     EATSTSNDPIDRIKEEGLKRSQVMATLSYLTDVIGPRLTGSPNMKRANEWTRDKLAAW
                     GLANAHLEAWGPFGRGWELKRFSAQIIEPQCIPLIGFPKAWSPGTDGTLVAPVVYFDA
                     KTEADFASYKGKLKGAIVLTSPIREVAARWEPLANRKTDSDLLALADAAEPNARGGRR
                     GGPGGPAAPAAGTAAASRPAGEQPRTGRFNNSPEMRAQMELQRKKTQFLAEEGVALLV
                     DSSTQGDGGTFFVQSATVPGAPLPTPGQTGRRVSPYDKDAPKITPQVVLSKEHYNRLV
                     RMCEQGEKLKMVVDLSVQFHDDDLMSYNTVAELPGSDLKDEIVMLGGHMDSWHSGTGA
                     TDNGAGVSVAMEAVRILQALNLKPRRTIRIALWSGEEEGLLGSRAFVKDHFGKTPSNN
                     LFGPAPPPATASATSTGNGNGNGATASTKNSSDSASKPEFDKFSAYFNLDNGTGKIRG
                     VYMQGNEGVRPIFRKWLQPFRDMGASTLTISNTGGTDHQSFDGIGLPGFQFIQDEIEY
                     GTRTHHSNQDVFDRIQGDDMKQAAVIMAAFIYNAATIDQRLPRKPAPNTESTTTAPAR
                     AAAAP"
     gene            complement(3899..>4138)
                     /locus_tag="VHS97_16745"
     CDS             complement(3899..>4138)
                     /locus_tag="VHS97_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449905.1"
                     /translation="ADLKRVIDDGKALYAFDRAAGRPIQPITDYFLAAIETVRANKLR
                     YAKAEARARANRPPRPPWFDVSQNVMAFHNSDDTD"
BASE COUNT          749 a         1303 c         1292 g          794 t
ORIGIN      
        1 gcgccgcgcc gacctccagc caggccccct cgggcgattc gcgatcgacg gccaccgggc
       61 cggcctgttc ggtcaaccgg gcgacccagt cgtcgagggt cgtcgggggc gcctgccatc
      121 ccggcagcgc gagcagttcg atcatcgggt tcagaccttg gggagggtcc agggggcgcg
      181 gtagtgctcg cgggccaggt gtcggttcgc ctcggggtgg ttcgtgacct cgttccgggc
      241 gtcccagtgc agcttctcgc cggtccagag cgcgatgttc cccaggtggc cgacggtcga
      301 gaggcggtgc ccgtactcga agtggcacga gcatcggccc cgcgacttga tcgcgtcgag
      361 ccattcgcgg tggtggccga ccgagcgcgg gatggtgggg gccgggatct cgaccgactt
      421 ccctttctcg ttgaagatcc ggtaggtgcc gtagtcggcg acgagcgtgg catcggtccc
      481 ctggaacatg atcccgagcc ccttgttgtc gagcccgtgg gtgctggcat cggtgtgact
      541 ccagaccatg aggaactttt gatcgccctt ctgatattcg taggtgacgt cgagcgtgtc
      601 gggcacctca gcgttatccg caagggcgta ccggccgcct gttgccgaga tggtgtgggg
      661 cgcatcgact tccatcgccc agtggaccaa gtcgacgatg tagcagcaga aatcggtgag
      721 gatgccgccg ccatagtccc agaagtaccg ccagttgaac gtgaagcggt tggcgttaaa
      781 aggacgagag ggtgcgggtc ccagccagaa gtcgtaatcg cacccgggtg gcggagagcc
      841 gtcttgagga cgccccatac cactgcggtc ggcagccagc cagacccgcg tcttggtgat
      901 ttttcccagg acgcccgaac gaacgatctc aacgactcgg cggtagtttt cgcctgcgtg
      961 gatcaagttg cccatctgcg tgacgcgttt gtacttttcc gcggcctgga ccatcgcccg
     1021 gccttcctgg attctgtgtg ccagtggttt ctcgcaatag acgtccttgc cggcctgaca
     1081 ggccagtatc gtcggtatcc catgccagtg atcgggcgtg gcgatcacga ccgcgtcaag
     1141 atccttgcgg tccagcaccc gacgaaaatc accgtatgtt tccgtccttt caccggccgc
     1201 ccgttgggcg cggcgcaagt ggggctcgta aacgtcgcac accgccgcga tctcgacgtc
     1261 gggaaactct ctgaacttat tgaccaggcc ggttcccatg ccgccgacgc cgatgaacgc
     1321 taatacgatt ttgtcggccg gagcgacttt cactggcgcg ctattcggtt tttcgcctgc
     1381 ggacagggcg gctttgcgtt ggtcgccggc ttttttgggg gcttcggctt gtgcaaccgc
     1441 gagtgccgag gccgccagga actcgcgccg attccaattg tgcatttgac ctccgttatt
     1501 cccgagcggg ctcgcctgca aacatctagc tttcatcgtg gatcgaaaac ccgaagtcaa
     1561 gccccacgga cgattttcga ccatgatccg cggttttctg cgatgttccc gcatcggaag
     1621 tcgggccggg tcggcgagat ttgtcgcttt acttaacatt gacattcgaa ggtggcggcc
     1681 agggacgtta cacttgagcg tctgcaacat agaatacggc cttcgaatcc agggctcctc
     1741 tcccagcctg aatgcatgtt tgtagagttt tgcaccagac ctttcgaatc gccccatcgg
     1801 ctcacccttt ctggagaccc gcgcttatgc cccgctttct gaggatgacc ctcgtcacgg
     1861 gcctgacctg cacgatgccg gttcttgccc tgtatgccca gcagcctccg ccgtccgcgc
     1921 ccgcctcgca gcccgcggcg aaaaccgcga ccaagagcga ggcaacctcg acctcgaacg
     1981 atcccattga tcggatcaag gaagaagggc tcaagcggtc gcaagtcatg gcgacgttga
     2041 gctatttgac cgacgtgatc gggccccggc tcaccggttc gcccaacatg aagcgggcga
     2101 atgagtggac gcgcgacaag ctggccgcgt ggggccttgc caatgcccat ctggaggcgt
     2161 ggggtccgtt cggccgtggc tgggagctca agcggttctc ggcgcagatc atcgagccgc
     2221 aatgtatccc cttgatcggt ttccccaagg cgtggtctcc cggcacggat gggacgctcg
     2281 tggcaccggt ggtttatttc gatgccaaga ccgaggccga tttcgcttcg tacaaaggga
     2341 aattgaaagg cgccatcgtc ttgacgagcc cgatccgcga ggtcgccgct cgctgggagc
     2401 cgctggcgaa ccgcaagacc gattccgacc tgctcgcgct ggccgacgcg gccgagccca
     2461 atgcccgtgg cggccgtcgc ggtggtccgg gtggtccagc cgcaccagcg gccggcacgg
     2521 cagccgctag ccgccccgcg ggcgaacagc cgcgaacggg ccggttcaat aattcgcctg
     2581 agatgcgggc ccagatggaa ctgcagcgca agaagacgca gttcctggcc gaggaagggg
     2641 tcgccctcct ggtcgattcc agcacacaag gcgacggcgg aactttcttt gtccaatccg
     2701 ccaccgttcc aggcgcgccc ttgcccacgc ccggccagac cggccgccgt gtctccccgt
     2761 acgacaaaga cgcccccaag atcactcccc aggtggtcct ttccaaggaa cactataacc
     2821 gcctggtccg gatgtgcgag cagggagaga agctcaagat ggtggtcgac ctctcggtcc
     2881 agttccacga cgacgacctg atgtcgtaca acacggtcgc cgagctcccg ggcagcgatt
     2941 tgaaagacga gatcgtcatg ctgggcggac acatggactc gtggcacagc ggcaccggcg
     3001 ccaccgataa cggggccggc gtctcggtcg ccatggaggc cgtccgcatc ctccaggctt
     3061 tgaacttgaa gccgcgacga acgattcgca tcgctctctg gagcggcgag gaagagggcc
     3121 tgttgggctc gcgcgcgttc gtcaaggacc atttcggcaa gaccccgtcc aacaacttgt
     3181 tcggaccggc gcctccgccc gccactgcct cggccacgtc cacgggtaac ggcaacggaa
     3241 acggcgcgac cgccagcacc aagaattcgt ccgattcggc gtcgaagccg gaattcgaca
     3301 aattctcggc atatttcaac ctcgacaatg gcacgggcaa gattcggggc gtttacatgc
     3361 aagggaacga aggcgtccgg cctatcttcc gtaagtggct ccagcccttc cgcgatatgg
     3421 gggcgtccac tctgacgatc tccaataccg gcggtaccga ccatcaatcg ttcgacggaa
     3481 tcggcctgcc cggttttcag ttcattcaag acgagatcga gtacggcaca cgaactcacc
     3541 attccaacca ggatgtcttc gatcggatcc agggtgacga catgaagcaa gccgcggtaa
     3601 tcatggccgc ctttatctac aacgcagcca cgatcgatca gaggctgccg cgcaagccgg
     3661 ctcccaacac ggaatcgacg accaccgcgc ccgcgcgggc cgccgccgct ccgtgacctt
     3721 atttcacttc gatggtgact tgagtaatcg tgtgtagaca tcgctcctct ttcccccccc
     3781 ttgtgaaggg ggggttaggg gggctggtca gggagctatc agccacagcg agatcaaagg
     3841 gggggtggtc agcgagctat cagcaacatt gagatcaaag ggggtggtcg ctgaacggtc
     3901 aatcggtgtc atcggaattg tggaacgcca tcacattttg agagacgtcg aaccaaggag
     3961 gccgcggcgg gcggttggcc cgggctctcg cctcggcctt agcgtagcgc agcttgttgg
     4021 ctctcacggt ctcgatcgcc gcaaggaaat agtccgtgat cggctggatt ggtcggcccg
     4081 cggcacggtc aaacgcgtag agtgccttgc cgtcgtcgat cacccgtttc aggtcggc
//
LOCUS       DATAQL010000963         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4831130, whole genome shotgun sequence.
ACCESSION   DATAQL010000963 DATAQL010000000
VERSION     DATAQL010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4831130"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1511
                     /gene="acnA"
                     /locus_tag="VHS97_16750"
     CDS             <1..>1511
                     /gene="acnA"
                     /locus_tag="VHS97_16750"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX3449906.1"
                     /translation="TGVPAIVDLAAMRDAMKRLGGDPRKINPLQPVELVIDHSVQVDE
                     AGTPQAFATNAEMEFQRNKERYAFLRWGQDAFGNFRVVPPDTGIVHQVNLEYLARVVF
                     GADGGKGSNWLAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLVPQV
                     VGFKLTGSLPEGATATDLVLTVTQMLRKRGVVGKFVEYFGSGLAGLPLADRATIANMA
                     PEYGATCGLFPIDAETIRYLELSGRQPAAIKLVEAYAKAQGMYHAADSPEAEYSDTLE
                     LDLSTVEPCLAGPRRPQDRVPLHNSKTAFEAALKELQSARPKNTPSSTSLPVIERFAS
                     EGGRTAIGVAEPEIEEGTVTPAPIKNGSVVIAAITSCTNTSNPSVMVAAGLLAKKAVD
                     KGLETQPWVKASLAPGSKVVTDYLQDAGLDKFLDQLRFNLVGYGCTTCIGNSGPLSPA
                     ISEAIQEHDLVAVAVLSGNRNFEGRINPDVRANYLASPPLVVAYALAGTMDID"
BASE COUNT          287 a          476 c          484 g          264 t
ORIGIN      
        1 tcaccggcgt gcctgcgatc gtcgacctgg ccgccatgcg cgatgcgatg aagcggctgg
       61 ggggcgaccc cagaaagatc aatccgctcc agccggtgga gctggtgatc gaccactcag
      121 tgcaggttga cgaagccggc acgccccagg cgttcgccac caacgccgag atggagtttc
      181 agcggaacaa agagcggtac gcgtttctgc gctggggcca ggatgccttc gggaatttcc
      241 gtgttgtacc gccggatacc gggatcgtcc accaggtaaa tctcgagtac cttgcccgcg
      301 tggtcttcgg ggccgatggt ggaaaaggct cgaactggct cgcatatccc gacactctgg
      361 ttggcaccga ctcacacacc accatgatca acgggttggg cgtcttgggc tggggcgtcg
      421 gcgggatcga ggccgaggcc gcgatgctgg gccagccggt gtcgatgctg gtcccgcagg
      481 tcgtcggctt caaactgacc ggctcgctgc cggaaggggc caccgccaca gacctggtgc
      541 tcaccgtgac ccagatgctg cgaaagcgag gggtcgtggg caagtttgtc gagtacttcg
      601 gcagcggact ggccggcttg cccctggccg atcgggcgac gatcgccaac atggcacccg
      661 aatatggcgc gacctgcggt ctgttcccga tcgacgcgga gacgatccgg tacctcgagc
      721 tctccggccg acagccggcc gcgatcaagc tcgtcgaagc ttatgccaag gcccagggca
      781 tgtaccacgc agccgactcg ccggaggccg agtattccga cacgctcgaa ctcgacttat
      841 cgacagtgga accgtgcctg gcgggtcccc ggcgcccgca ggaccgggtg ccattgcaca
      901 attcgaagac ggcgtttgaa gccgccctca aagagctgca gtcggcccgg cccaagaaca
      961 ctccgtcgag cacgtctttg ccggtgatcg agcggtttgc ctccgagggt gggcgtacgg
     1021 cgatcggcgt tgccgagccg gagattgaag aaggtacagt tacccccgcg ccaatcaaga
     1081 acggctcggt cgtgatcgcg gcgatcacga gttgcaccaa cacgtcaaac ccgtcagtca
     1141 tggtggcggc aggacttctt gccaaaaagg ctgtcgacaa agggctcgag actcagccgt
     1201 gggtcaaggc gagcctggcg cccggctcga aggtcgtcac cgactacctc caagacgcgg
     1261 ggctagataa atttctcgac cagcttcgat tcaaccttgt cggttacggc tgcacgacgt
     1321 gcatcggcaa ttcggggccg ctctccccgg cgatcagcga agcaatccaa gaacacgatc
     1381 tggtcgccgt cgccgtcttg agtggcaacc gcaacttcga gggacggatc aatcccgatg
     1441 tgcgggccaa ttacttggcc tcccctccct tggtggtggc ttatgccctg gccggcacaa
     1501 tggacatcga c
//
LOCUS       DATAQL010000964         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2846311, whole genome shotgun sequence.
ACCESSION   DATAQL010000964 DATAQL010000000
VERSION     DATAQL010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846311"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1031)
                     /locus_tag="VHS97_16755"
     CDS             complement(174..1031)
                     /locus_tag="VHS97_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449907.1"
                     /translation="MATGRAVGRELNSIVADPSLQLDLSSEDSNTNGLFYLASGAWST
                     GASDGALAWEENSTDPFLPGSITAGGLSLNILLADPDVPIRTDQDRLEQVAELVPLPE
                     TSLALAATLWTVPSDSPTSGAQSDLSSATATDSAAPLASPSTPVNFVIGMDQALEQTY
                     RDIREGILYSHDGPPGIESPPDGQDELLEWQAPILPAGRGGSPVNETGSARPAGTGAF
                     GEAGRMTLQSHEESPLDSHDGSPVVLWALPMISVVSVSTVIAGWFWNKRQRLRRLGFG
                     GAKKPNGRA"
     gene            complement(2144..>4123)
                     /locus_tag="VHS97_16760"
     CDS             complement(2144..>4123)
                     /locus_tag="VHS97_16760"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449908.1"
                     /translation="LGQAQHRRDQKASWERARDRLQQFLILRDDALFQDTQLTGLDPS
                     ENVAVVHKSTMAALNLFTAGSQRGDQWKLAPLHPSLTKQERENVTRGCYEMLMVLAES
                     VAQPLSGESSSAQAREALQILERAALLRHQPTHAYHLRRAVCLDRAGDAEGAKRERSA
                     AELIQPDGAFDHFLTGLEQYKRGYMTQAKRHFALALLDQPNHFWAQCLLAICDLNSRP
                     VHAEGAKAYLTACLQSHPELPWLYLLRGFASGQMGSMASSQSEAVDCFNAAEADYREA
                     LGRDSDGRFRYALLVNRGLVRLQSRKLDEAIVDLQEAIELDPQQLSAYVTLAQIHRHR
                     REVDLALETLGRGIALKPNLAALYRTRARWTLERPSVTPDARALALADLEEAIRLGAP
                     DSRELAKDHAERGRVLLMDKQYQQALDACDKAQRIDPKDDKVHLYRVGALLELKRYQE
                     AIDACDLYLRTGRSSADLLGLRGLAKARRNDFAAAIEDYTLVLALQPSAPVPRARRGW
                     AYLVSGAPHLALRDFEEAIRLDPYSGDYYSGRGSALVALGRHREAVTDAEESLRHGES
                     EGRLHFSAARILALAAESAKKEARPRDPSQLDKVQNYRDRALALLGQAVEQTPTERRA
                     RFWREVVQSDHAFTTIRRHPAFARIATAACQPHAQ"
BASE COUNT          803 a         1217 c         1305 g          798 t
ORIGIN      
        1 aagacgaccg agcgctgacg gctcgatgcg atcaacgttc caggattttg taatcgggcc
       61 ggacaacacg ccaggtgtgt gcccgtcgtg aaaaatcacg gtagcgcgcg gttcgagccc
      121 gcccccgcag cttggactgg ctttgctctg cgcgtcattt cggctttttt tttttatgca
      181 cgtccattgg gctttttggc gcctccgaag cccagccgcc gcaatcgctg ccgtttattc
      241 cagaaccagc cggcgatcac agtggaaacg gacacgactg aaatcatcgg caaagcccag
      301 aggactacgg gggacccgtc gtgggaatcc agcggagact cttcatgact ttgaagggtc
      361 atcctccccg cctcgccgaa ggcgccggtt ccggctggtc gcgctgatcc cgtctcattg
      421 accggtgatc ctccccggcc ggctggaagg atcggcgcct gccattctag cagctcgtcc
      481 tgcccgtccg gaggactctc gatcccgggt ggcccgtcgt ggctgtacag gattccttcc
      541 cggatatcgc gataagtctg ttccagagct tggtccatgc cgatgacgaa attcaccggt
      601 gttgatggag aagccaaagg tgcggccgaa tccgtggccg tagccgacga caggtccgat
      661 tgggctccgg acgtcggaga atccgacggg accgtccaca aagtggctgc cagagccagc
      721 gatgtctctg gcagcggaac aagctcggcg acctgctcga gcctgtcctg gtccgtccgg
      781 atcggcacat cggggtcggc cagaagaatg ttcagcgaaa ggccaccggc ggtaatcgaa
      841 cccggcaaaa aggggtcggt cgagttctcc tcccaggcga gcgcgccgtc ggaagcgccg
      901 gttgaccatg cgccgctcgc gagataaaag agcccgttcg tgttggagtc ttcactactc
      961 aaatcgagct ggagcgaggg gtctgcgacg atagagttca attcgcgacc gactgctcga
     1021 ccggttgcca tcgctgtgga catcgacgtt gaaaaaccat cgcttggtcc gacagtttcc
     1081 atgggtccgc caatgctcat atcggacact tcagaatcga ggttcgtctg agagcctaaa
     1141 agccccggat tatatcccct cgggatgccg ggagactgta aaagaaatga gtaaaacgcg
     1201 ctcccgtcgt taacttgatt ggaattcagg agagtgcccc caaccaagcc ttccacgacg
     1261 gttcccccgt tggagagatc cgggagcgga agttcggcaa tttttgaccc gccgttccac
     1321 tgggtcggag cctgagtcga tccgagtgcc acgctcgagc cggacccagg cgtcggggtc
     1381 gtcgatgttg aaaagccatc gaatgatgcg atcgtttgca tgggtccgcc attgctcata
     1441 ttggacgctg catcattgag gttcgtctga gagcctgatt gcgacggacc agatcccgtc
     1501 gggacgccga gaacagactg tagaagagag gggctaaaaa cgctcccggc gtcaatatgg
     1561 acggacgtaa aaataacacc ctcaatcaaa cctaccgcga cggttgcccc gctggagaga
     1621 tcagggagcg aaagtgcggg gattcgtgcc gcgccgttcc actgggaagg aatcgaagtc
     1681 ggaccaactg ccaggctcca gccggacccg agcgtcaggt tcgtcgggac aaccacttca
     1741 ctgctcggcg cattcgtaag aaatccctca agcgtgttcg gcgaggacgg ggacgggagg
     1801 tcaatcaccg atctcggcaa atttgtgtcg acatcccaag aaggtgtcga cggactggga
     1861 gcaatcgtct gctggacgtc ggcgacctgg agtgttgtcc attccaatgt ctgcaggcgg
     1921 cccgggatca aaataccgat ttccacggcc tgaaaggcga cgatcgtttc aattcccgtt
     1981 cccgatcctg acgaaagagg agtgcgattc tcgagcgact ccacctggag gctacgcccg
     2041 acgcgaggcc ggcgttcccg agccattggg cccttactgc cgggccgatg taagatccaa
     2101 tgaatgagca tcaatgaatt ccctaaaaac ggtgccaggc gcgttactga gcgtgaggct
     2161 ggcacgccgc cgtggcgatt cgagcgaaag cgggatgtcg gcggatcgtc gtgaaggcat
     2221 gatcggactg gacgacctca cgccaaaatc gggcacgccg ttccgtggga gtttgctcga
     2281 cggcctggcc gagtaacgcc aaggcgcgat cccggtagtt ctggactttg tccagctgcg
     2341 acggatcgcg cgggcgcgcc tcttttttgg ctgattcggc ggccagggcg aggatgcgcg
     2401 cggcgctaaa atgcagtcga ccttccgact caccgtggcg cagcgactcc tcggcatcgg
     2461 tgacggcttc acggtgacgt cccagcgcga ccaaggccga accccgcccg ctgtagtagt
     2521 ctccgctgta gggatcaagg cggatggctt cctcgaagtc gcgcagtgcc aagtgtgggg
     2581 cgccagatac cagataagcc caccctcgcc gggcacgcgg gacaggcgca gacggttgga
     2641 gggccagaac cagagtgtag tcttcgatcg cagccgcgaa atcattgcgc ctggctttgg
     2701 cgagtccgcg caggccgagg agatcggccg acgaacggcc cgtgcggaga taaagatcgc
     2761 acgcgtcgat cgcctcctgg tatcgcttta gctccagcag tgccccgacg cgatagaggt
     2821 ggaccttgtc gtctttagga tcgatccgtt gggccttatc gcaggcgtcg agtgcctgtt
     2881 gatactgttt atccataagg agaacgcgac ctctctcggc gtggtccttc gccagctcgc
     2941 ggctgtcggg cgctcccagt cgaatggctt cttccagatc ggccaaggcg agggcgcgcg
     3001 cgtccggcgt cacgctaggg cgctccaggg tccaacgggc tcgggtccgg tagagggcgg
     3061 ccaggtttgg cttgagcgcg atgccgcgac cgagtgtttc gagcgcaaga tcgacctcgc
     3121 gccggtgccg atgaatctgg gccagtgtga cgtaggcgct caactgctgc gggtcaagtt
     3181 cgatcgcctc ttgcaggtcg acgatagcct cgtccagctt tcgactttgt aaccggacga
     3241 ggccgcggtt gaccaagagg gcgtaccgga acctgccgtc cgaatcgcgc cctagtgcct
     3301 cgcgatagtc ggcctcggcg gcattgaagc agtcgaccgc ctcgctctgg ctcgatgcca
     3361 tcgaacccat ctgtccagac gcgaagccgc gaaggagata aagccagggc aactcgggat
     3421 ggctctgaag gcacgcggtc agataggcct tggccccttc ggcgtgcacg ggtctggaat
     3481 tcaggtcgca gatggccagc aagcactggg cccagaaatg gttcggctgg tctagcaggg
     3541 ctagggcgaa atgccgcttg gcttgcgtca tgtagccgcg tttgtactgt tcgagaccgg
     3601 tcaggaagtg gtcgaatgcc ccatccggtt ggatgagctc ggccgccgac cgttcacgtt
     3661 tcgccccctc ggcatcgccg gccctatcta ggcagacagc tcgtctcaga tgataggcat
     3721 gagtcggctg gtggcgtagg agggcggcgc gttccaggat ctggagggcc tcgcgagctt
     3781 gcgcgctcga cgactcgccg gaaagaggct gggcaacgga ctccgccagc accatcagca
     3841 tctcgtagca gccgcgggtg acattctccc gctcttgttt ggtcagagat ggatgaagcg
     3901 gcgccaattt ccactgatcg ccgcgctggc tgcccgcagt gaacaaattc agcgcggcca
     3961 tggttgactt gtggacaacc gccacgttct cggacgggtc gaggcccgta agctgtgtgt
     4021 cttggaacag ggcgtcatca cggagaatga ggaactgctg aagacgatcc cgcgcgcgct
     4081 cccatgatgc tttctgatca cgtctatgtt gtgcctgtcc gag
//
LOCUS       DATAQL010000965         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4371484, whole genome shotgun sequence.
ACCESSION   DATAQL010000965 DATAQL010000000
VERSION     DATAQL010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4371484"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            625..1971
                     /locus_tag="VHS97_16765"
     CDS             625..1971
                     /locus_tag="VHS97_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449909.1"
                     /translation="MASQSIQKTSHARTQQSGRRTGRFLASLRWRGWSCLLGLAFLTL
                     LLGYWGSRQIRPDGQSLDLLWEQAEQDLSAGRFESVEAAVKRLERSRPPTPLDWFLRA
                     QLALARKEDDQALEYLTRVPDEHYMAAQARLIAGQMELRRNRARLAEEWFQAALKLDS
                     RLIQAHRELIYIYGMQLRRAALSAEFMALSRLTNLTFDNAFHWGLLRNNSWEPGTAVE
                     SLAGYVAADPFDRWSRLALAENYRRMGRLDDAESAIGTLASDDSEAISLRGRMALDRQ
                     DHEEAGRLLALGPRDQPLLARLRGTLALGRRDVPEALANFRIAYAADPENREALFGLI
                     AALELSHDDKAAEPLRERARRYDRLNSLIQRAAVAQARGDADLMRQLGEACAALGRKE
                     EARAWYNLAISRDPLDSIAQQALFRLSETTEKVPQPPRVVPQPPPVLPNHNEPDSH"
     gene            2032..3585
                     /locus_tag="VHS97_16770"
     CDS             2032..3585
                     /locus_tag="VHS97_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449910.1"
                     /translation="MTTFSGTVSFYVLTALSLWLGWGIRGNFGHEYGAMMPGALAAMA
                     AVLLSGRDDWQRRIVYFAMFGALGWSFGGSISYMQVVAYTHSGDSASVLYGFACLFVI
                     GFLWAALGGAGTALPAVVDQVFLNSLFLPMAAVFLLWWLQGVVIEPALKQHGFELFWY
                     DTDWMAATLALVAALLVWAVRRRLDFGNSLVLHLAAGWWAGFLVLVLLLRLHMTPPRG
                     DNWAGCMGMTVGLLWYCRQTGLHELARAAVYSGFIGGIGFAAASMLKLVEVTSGYQTN
                     WHSILEQTTGLFNGIGIAVVVAALARRTRPLADLPDRRPWTEPLALGFVLLGITYLNL
                     RKNVGQWVDAKSMPAEMAGLSAWAWFNWGYAALAVALVVLWMRHRRRPLAVVPATWSG
                     KGQILYLGLLWWLVAGNFERALVAFRPERLVTEGVLYLVALACTLVLLLAPGQTGELT
                     KREAWTPAARGRSRLAATVAVGLIAATLSILADWAIVRAIYGDRFAGHAGLHTRFRTT
                     ATTNPQENR"
     gene            complement(3551..>4425)
                     /locus_tag="VHS97_16775"
     CDS             complement(3551..>4425)
                     /locus_tag="VHS97_16775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3449911.1"
                     /translation="ELNVIDGYHFMALPYVEGTALRDVIKCRRAYLSGDDTRKVHQFV
                     TLSDEDYLAGMTRTLAKAARALASVHDQRIAHRDIKPANILLDNHRSEGVYVCDFGLG
                     RDLDVATAEQMRDGAGTPLYMAPERLLRLGADEIKCDIYSLGVTLCEALTLDRPFRIP
                     EDLALPALAHFLASAEPVPPRVLEPQFPADLEAIISKAMARNPRDRHDSAAELAGDLE
                     RFASDWSFRCGRRGLEAQRRPAGPHSHKLRGRVLAVLDQTRRSFAGNSSRANHAPDQS
                     CECFNDFPAGWSSR"
BASE COUNT          758 a         1349 c         1399 g          919 t
ORIGIN      
        1 gattcggacc gtcccgatca ccatcatcca ggaactcagt tcaatgagcc gctcgtgtat
       61 cgtcgtcgat ccacggtcac gctcccggtt ggcgatcgag tgatgatcac cctccatcgc
      121 ccggacgagc tccatgagtc actcctcgca gagagtatca accacgcatt ctttgatgta
      181 gaggcaaacg tcgacgtgat ggcaaaccaa gctcatgacc tgtcattcgt tagtcaatca
      241 acggcttcga acgccgctta tctcttatac ccggtttcac cgtgagttca atcgcgctgc
      301 gatctttgtg gtgattgcaa cgaacattcg gccaacttgc actacgtcga tgcgcatcgg
      361 gccgagctta aggccacgcg gcgtttttgt cgggcaacgt gtagtggtcg tggtctcgac
      421 aagacgcagc ggcaaacatc gccgcgcggc tgtcatggat tttttcgcct taacgcgaca
      481 cgggtctgtg tgggcgttcc gctccaagat ttgataggat aatcttttcc gcgggcggtt
      541 tccgacaaca aaggcctcgg aagtccaccc gttcaccggt cctcttcgct ttcggctggc
      601 tcgagcgacg cggcgaattc gcgcatggca tcacaatcga ttcaaaagac ctcgcatgct
      661 cgtacgcaac aatcggggcg ccgaacagga cggtttctgg cttcgctccg gtggcgcggc
      721 tggtcttgcc ttctcggtct ggcgtttctc acgttgcttc tgggctactg gggctcgcgg
      781 cagattcggc ccgacggaca aagcctcgac ttgctgtggg agcaggcgga acaagacctc
      841 tcggccggcc gatttgagag tgtcgaagcg gccgtcaagc gactcgaacg gtcgcgtccg
      901 ccgacgccgc tcgactggtt cctccgcgcc cagctcgcgt tggcgcgcaa agaggacgat
      961 caggcactgg agtatcttac gcgcgttccg gacgagcact acatggccgc tcaggcgcgg
     1021 ctgatcgccg gtcagatgga gttgcggcgc aaccgcgccc gcctggcgga ggaatggttc
     1081 caggccgcac tcaaactcga ttcgcgcttg atccaggcgc accgtgaact gatctacatc
     1141 tacggcatgc agctccggcg agccgcgctc agtgcggaat tcatggcgct ctcgcggctg
     1201 acaaacctga cctttgataa tgcgtttcac tggggcttgc tgcggaacaa ttcatgggag
     1261 ccgggaaccg cggtggaatc ccttgccggt tacgtcgcgg cggacccttt cgatcgttgg
     1321 tcacggctgg cactcgcgga aaactatcgg aggatgggcc gtctcgacga cgcggaatcg
     1381 gcaatcggca ccctggccag cgacgactcg gaggcaattt cgctcagggg ccggatggcg
     1441 ctcgaccgcc aggatcacga agaggccggc cggttgctcg cgctgggccc gcgcgatcaa
     1501 ccgctcctgg cacggctgcg aggtacactt gctctgggga ggcgagacgt tcccgaggcg
     1561 ctggccaact tccggatcgc ttacgcggcc gaccccgaga atcgcgaggc actcttcggg
     1621 ctcattgctg cccttgagct ttcccatgat gacaaggccg cagagcctct tcgtgaacgc
     1681 gctcgccgct acgaccgact gaacagcctg attcagcgcg cggccgttgc ccaggcgcgc
     1741 ggggacgccg atctgatgcg ccagctcggg gaagcctgcg cggccctcgg gcgcaaagag
     1801 gaagcccgtg cctggtataa tctggctatc agccgcgacc cgctagactc cattgcacag
     1861 caagctttgt ttcggttaag cgaaacgacg gaaaaggttc cccagccacc acgcgtcgtc
     1921 ccccagccac cacccgtcct ccccaatcat aacgagcctg attcgcactg agccgtacgc
     1981 ggcacgattc atcgagcctg aatcgaacaa aagggtttaa tcgagatgtc gatgacgact
     2041 ttttccggca cggtttcgtt ctatgtcttg acggctcttt cgctctggct cggatggggt
     2101 attcgcggga atttcggtca tgaatacggc gccatgatgc cgggtgcact cgcggccatg
     2161 gcggcggtct tgctttcggg ccgtgacgac tggcagcgcc ggattgttta tttcgccatg
     2221 ttcggcgcgc tcgggtggtc tttcggcggc agcatttcct acatgcaagt cgtcgcgtac
     2281 actcactcgg gagattcggc gagcgtgctt tatggattcg cctgcttgtt cgtgatcggc
     2341 tttctctggg ccgccctggg aggagcgggg accgccttgc cggccgttgt ggatcaagtg
     2401 tttttgaact cgttgtttct gcccatggct gctgtctttc ttctctggtg gctacaaggc
     2461 gtggtcatcg agccggcact caaacagcat ggtttcgagc tattctggta tgacaccgac
     2521 tggatggcgg cgacgctggc cttggtcgcg gcgctcttgg tttgggccgt caggcgcagg
     2581 ctcgacttcg gcaattcgct ggtcttgcat ctcgccgccg gctggtgggc tggcttcctc
     2641 gtcttggtat tattgctgcg tctgcacatg actccgcctc gcggcgacaa ttgggccggg
     2701 tgtatgggaa tgaccgtcgg gctcttgtgg tattgccgcc agacagggct tcatgagctg
     2761 gcacgcgccg cggtttacag cggtttcatc ggcggcatcg gcttcgcggc ggcatcgatg
     2821 ctcaagctgg tcgaggtcac tagcggttac cagaccaact ggcatagcat cctggaacag
     2881 acgacgggac tttttaacgg catcggcatc gcggtcgtcg ttgccgctct ggcgcggcga
     2941 acgcgaccgc tcgccgatct acccgaccgc cggccgtgga ccgagccgtt agccctgggg
     3001 ttcgtcttgc tcgggatcac gtacctcaat ttacggaaaa acgtgggtca gtgggtcgac
     3061 gccaaatcga tgcctgccga gatggccggg ctatccgcgt gggcgtggtt caactgggga
     3121 tacgccgcgc tggccgtggc gctcgtcgta ctctggatgc ggcacaggag gcgtccgctc
     3181 gcggtcgtgc cggcgacctg gtccggtaag ggacagattc tctatctggg gttactctgg
     3241 tggctggtcg ccggcaactt cgagcgggct cttgtggcct tcaggcctga gcggctcgtg
     3301 accgagggag tcctttatct ggtcgccctg gcttgcacgc tcgtgctgct cttggcgccg
     3361 ggccagaccg gtgagttaac gaagcgagag gcctggacgc cggccgcgcg cgggcgatct
     3421 cgactggccg caaccgtcgc cgtgggtttg atcgcagcaa ctctgtcgat cctcgccgac
     3481 tgggcgattg tacgggcgat ctacggcgac cgattcgccg gccacgccgg gcttcacacc
     3541 cgtttccgca ctaccgcgac gaccaacccg caggaaaatc gttgaagcat tcgcagcttt
     3601 ggtccggagc gtggttggca cggctcgagt taccggcgaa cgaacgccga gtctggtcga
     3661 gcaccgccag gacacgaccg cgcaatttat gggaatgcgg cccggccggt cggcgctgcg
     3721 cctccaggcc tcggcgtccg caccgaaacg accaatccga ggcgaatcgc tcgaggtcgc
     3781 cggccagctc ggcggcggaa tcgtgacgat ctcgcgggtt tcgcgccata gccttgctga
     3841 tgatcgcttc caagtccgcg ggaaattgcg gttcgagcac gcgcggcgga acgggttctg
     3901 cactggccag gaagtgggcg agcgccggca aggccagatc ctctggaatg cggaatggcc
     3961 gatccagggt cagcgcctcg caaagcgtga cccccaggga atagatgtcg cacttgatct
     4021 cgtcggcgcc gagccgcagc aaacgttcgg gcgccatgta caaaggagtg ccggcgccgt
     4081 cgcgcatctg ttccgcggtg gcgacgtcga gatcgcgacc caggccgaaa tcacagacgt
     4141 aaaccccttc ggaccgatga ttgtcaagga gaatgttcgc cggcttgatg tcgcggtggg
     4201 cgattcgctg atcgtgtacg ctcgccaggg cgcgcgcggc cttcgccaaa gtgcgtgtca
     4261 tgccggccag ataatcttcg tcggatagcg tcacgaattg gtgcaccttc cgggtgtcgt
     4321 cgccggatag ataggcgcgc cggcatttaa tcacatctcg cagggcagtc ccttctacgt
     4381 agggcaaggc catgaagtga tagccgtcga ttacgttgag ctcga
//
LOCUS       DATAQL010000966         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_403348, whole genome shotgun sequence.
ACCESSION   DATAQL010000966 DATAQL010000000
VERSION     DATAQL010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403348"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /gene="asnB"
                     /locus_tag="VHS97_16780"
     CDS             <1..1536
                     /gene="asnB"
                     /locus_tag="VHS97_16780"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX3449912.1"
                     /translation="LARDRLGQKPLVYRHDGRQLVFASELKALLALPENTVPRQLDPL
                     ALDHYLCYGYVPAPRTILDGTHKLPPAHYAVWHDGELALERYWQPDWNVEHERPIEED
                     IEELRATLGDAVREQMIADVPLGAFLSGGIDSTIIVGLMQRASSQQVKTFAIGFPDPN
                     YDETSYAEMAARHLGTEHQTFIVEPKAWETLPALARQFDEPFADSSALPTWYVSRETR
                     RSVTVALTGDAGDELFGGYDRYRALALTEVFQQLPDVPRRLLGGTMTRVLPRGARSKS
                     GLRKLQRLFEHINEPAESRYLGWMTTFDEAARMALYSEAQLDSLAIAAGRLPYPAEAD
                     PATSLFNAFAAAGRRDRVARAMAADIVTYLPGDLLVKVDLASMAHSLECRAPFLDHRV
                     VELAAAMPLDRKIKLRSGRSKVVLKQAFSEFLPPAIKTRAKMGFGVPISRWFRDELQK
                     ELTDVLLDPVCLNRGLFRPAAIEKLVADHVLGKREHSFRLWALLMLELWFRTHLDPPA
                     SKP"
     gene            complement(1630..>2266)
                     /locus_tag="VHS97_16785"
     CDS             complement(1630..>2266)
                     /locus_tag="VHS97_16785"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449913.1"
                     /translation="PGGFTPPQGTPTGQPVVQAMSNYVPCCGMWGNPYDPWTRDALAA
                     TDVMGGAVGAITTQRATKLASITDGLSNTIVYGERAQGISSPTSISQDEYLGMWWDAS
                     WYAFRQFDGEYGINAHLKYSGLISGGCFWFPVEAASSMHPGGANFAFCDGSVKFLKDT
                     INSWPIIATCDAPGIAYDATTGYELIGTAVPGVYQKLTSRSYGEVISSDAY"
BASE COUNT          401 a          705 c          703 g          457 t
ORIGIN      
        1 ctggcgcgcg accggctcgg ccagaaaccc ctcgtctatc gccacgatgg ccggcagctc
       61 gtttttgcca gcgagctcaa ggccctgctg gccttgcccg agaatactgt gccgcggcag
      121 ctcgatcccc tggcgctcga tcactacctc tgctatggct atgtaccggc tcctcggacc
      181 attctggacg gaacgcataa gcttccgccg gcgcattatg ccgtatggca tgacggagag
      241 cttgcgctcg agcgttactg gcaacccgac tggaacgtcg agcacgagcg tccgatcgaa
      301 gaagacatcg aggagctgcg cgcgacactg ggtgatgccg tgcgcgagca gatgatcgcc
      361 gatgtgccgc tgggggcgtt tctttcggga ggcatcgatt cgacgatcat cgtcggcctc
      421 atgcagcggg cgtcgagcca gcaggtgaag acctttgcga tcggctttcc ggatccgaat
      481 tatgatgaga caagctatgc cgagatggct gcgcgccacc tgggaaccga acaccagacg
      541 ttcattgtcg aaccaaaggc gtgggagacc ttgccggcct tggcacggca gtttgacgag
      601 ccgttcgctg atagctcggc cctgccgacc tggtacgtct cccgcgagac caggcgctcg
      661 gtcacagtgg ccctgaccgg cgacgccggc gacgagcttt tcggcggata cgaccggtat
      721 cgggccctgg ccttgaccga ggtatttcag caactgccgg atgtcccgcg gagacttctg
      781 ggtggaacga tgactcgtgt cttgccacgc ggagctcggt caaagagcgg gctgcgcaag
      841 ctccagcgtc tcttcgagca catcaacgag ccggcggagt ctcgttacct cggctggatg
      901 acgaccttcg acgaggcggc ccggatggcc ctttattccg aagctcagct cgattcgctg
      961 gccatcgctg cggggcggtt gccttacccc gcggaggcag atcctgctac atcgctcttc
     1021 aacgcatttg cggccgccgg ccgccgcgat cgtgtcgcgc gcgcgatggc ggccgatatc
     1081 gtgacctacc tacccgggga ccttttggtc aaggttgacc tggccagcat ggcgcatagc
     1141 ctcgagtgcc gcgcgccgtt tctggaccac cgggtcgtcg agctggccgc tgccatgccg
     1201 ctggaccgaa agatcaagct acgctcaggc cgttccaaag tcgtgctcaa gcaggcattc
     1261 tccgaattcc tcccgccggc catcaaaaca cgcgcgaaga tgggctttgg cgtacccatc
     1321 agccgctggt ttcgtgatga gctgcagaag gagctaaccg acgtcctgct tgatcccgtc
     1381 tgcctgaatc gaggtttgtt ccggcccgcg gcgatcgaga aactcgttgc cgaccatgtt
     1441 ctcgggaaac gcgaacattc atttcgcctc tgggcgcttc ttatgctcga attgtggttt
     1501 cggactcacc tcgatccgcc ggcttcgaaa ccgtgaactc ttctcgccgt actggcgcga
     1561 ttcacgctgc gcggttgacc agatcggtag cgggctctga gttcaggccg ctctttgaca
     1621 atatggttct cagtatgcgt cggagctgat cacctcgccg tatgaccggg aggtcagctt
     1681 ttggtagacg ccggggacag cggtgccaat cagctcgtag cctgtcgtgg catcgtaggc
     1741 gattccaggt gcatcacacg tcgcgatgat cggccaagag ttgatggtgt ctttgaggaa
     1801 cttcaccgag ccatcgcaaa acgcgaagtt ggccccgcca ggatgcatgc tcgatgccgc
     1861 ctcgacgggg aaccagaagc agccgccact gatcaggcca gaatacttca agtgggcgtt
     1921 gatcccgtac tcgccgtcga actggcggaa tgcgtaccag cttgcgtccc accacatccc
     1981 caggtattcg tcctgactga tcgacgtggg cgacgaaatg ccttgagctc gctcgccgta
     2041 gacgatcgtg ttgctcaagc cgtcggtgat gctggcgagc ttggttgccc tttgagtcgt
     2101 gatcgcgccg acagcgccgc ccattacgtc agtggcggcg agggcgtcgc gcgtccaggg
     2161 atcgtacgga ttgccccaca tgccgcagca cggaacatag ttcgacatgg cctgaacgac
     2221 cggttggccg gtgggcgtcc cctgcggagg cgtgaatccg cccggg
//
LOCUS       DATAQL010000967         4382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3151551, whole genome shotgun sequence.
ACCESSION   DATAQL010000967 DATAQL010000000
VERSION     DATAQL010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3151551"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /gene="pgi"
                     /locus_tag="VHS97_16790"
     CDS             <1..501
                     /gene="pgi"
                     /locus_tag="VHS97_16790"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244526.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX3449914.1"
                     /translation="QHSFYQLIHQGTRLIPCDLIGFAHTLNPLGDHHDLLMSNVFAQS
                     EALAFGKTADEVKAEGTPDWLVPHRTFEGNRPTNVLLSERLTPEILGTLVALYEHSVF
                     TQGTIWHINSFDQWGVELGKVLAKRIIPELKSQEEPKLAHDSSTNALIRRYRSLRNTN
                     RKTPGA"
     gene            complement(620..1324)
                     /locus_tag="VHS97_16795"
     CDS             complement(620..1324)
                     /locus_tag="VHS97_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3449915.1"
                     /translation="MRMTLGWTGLRTAVVALGLGACAQTGVNAAAISATSSDAVLGYS
                     TAGSVDTTKAGDGVSGTNVISFVPITNSQVSATSNVPLGSFQVASELTGQSTTYTNTP
                     FSISFIPATVAGETPSTSSPVMITGVLNGTISGSNQSSVVATFNTFNGVSTVNTSAGT
                     FSSTLTIPQGSTLPLVPSSAGNGATTVEGMIATTLSSNNEVPAPEPSTIALFLSTVCG
                     LGLRKYVLARRQRSQA"
     gene            complement(1773..>4382)
                     /gene="priA"
                     /locus_tag="VHS97_16800"
     CDS             complement(1773..>4382)
                     /gene="priA"
                     /locus_tag="VHS97_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX3449916.1"
                     /translation="SRISPTVSARAPSGPSWQKRVTAGDLRSSLLARTQILARIRYND
                     AKLENDLGEQAPSMNVLQAKADGELDDGTAARAGSDQGAEGERSAVVSPRQSAWFGMS
                     DADQTLAEGPFVGVVFNRPIEQVLTYRVPSRLKSVIRAGQRVRVPLGRGTKPAVGYVV
                     RVDASAPADLDPARIKEVVEVLDPLPLIDGTMLQLTRWIADYYACSWGQALDAVVPAG
                     VKKHAGTRIGTFLLVPAEIRADLREEILKRRLPPKQTAVLEVLCRSDEPLTVVDVCRM
                     AKCTPVPIQALRKEGLVGSVKRRLPVGLSKTISPDDAPPALDDTDAPSQKGKTAKDLP
                     RPVLTAEQAVTLDHLVPAIESGGFGPFLIHGVTGSGKTEVYLAAIEEVVARGREAIVM
                     VPEISLTPQTIRRFRRRFASVAVLHSHMSDAERHRHWQSIASGEVQVVVGARSAVFAP
                     ARRLGLLVVDEEHESSFKQETTPRYNARDVAVMRAQMEGVPVLLGSATPSLESWRNAE
                     RGRYTRLAMPSRVGGRPMPRVEIIDMRNEKAAVAGLSESLRQAMVQALLEGGQIILLL
                     NRRGFHTFVLCPRCGHVVKCHACDVAATYHKSRHTLICHTCDAERACPPACPGCGAPA
                     LHYGGIGTERLEREIRAAFPDVVARRMDSDTMRSPGSHEEVLGAFKAGLVRILLGTQM
                     IAKGLDFPNVTLVGVVNADTALHLPDFRAAERTFQLVAQVAGRTGRGDRPGRVLVQTY
                     APDHPAIRSAAKHAFLEFAQNELPEREKFGVPPFGRLVRVIARGPEDKAVFAYMKDLA
                     AAFRLAADPSVRVVGPAPAPIIKIRNLFRFHLQLRCANSRPLQNLVNMVPRNHPAQHG
                     IELAIDVDPITML"
BASE COUNT          848 a         1411 c         1297 g          826 t
ORIGIN      
        1 caacattcgt tttaccagct catccaccag gggactcgcc tgatcccctg cgacttgatc
       61 ggattcgctc acacgcttaa ccccttgggc gaccatcacg atctcttgat gtccaacgtg
      121 ttcgcccagt ccgaggccct cgctttcggc aaaacagccg atgaggtgaa ggcagaggga
      181 acgcctgatt ggttggtgcc gcaccgcacg ttcgagggaa acaggccgac caacgtcctc
      241 ctttccgaac gcctcacgcc ggaaatcctc ggcacgttgg ttgcgcttta cgagcacagc
      301 gtcttcaccc agggcacaat ctggcacatc aactcgttcg accagtgggg tgtcgagctg
      361 ggcaaggtgc tcgccaagcg gatcatcccc gagctcaaga gccaggagga gccaaaactg
      421 gcccacgaca gctcgacgaa cgcgctcatt cgccgctacc ggagcctcag gaatacgaac
      481 agaaaaaccc ccggcgcgtg agcgccgagg gtcgggcgga ggcagtttcg cggattctca
      541 tccgttccag ccgagagtga ccagatcgct cctctctctt tcaccgagtc gcgaacggcc
      601 agatctcgtt gtaattcgtt caagcttggc tacgctgccg gcgtgcgagt acatattttc
      661 gcaggcccag gccgcagacg gtgctcagga agagagcgat cgtgctgggt tctggcgcag
      721 gaacctcatt gttggacgac agtgtcgtcg caatcattcc ttcaacggtg gtcgcaccgt
      781 tgcccgccga cgagggcacg agcggcagcg tgctaccttg aggaatggtg agcgtgctgc
      841 tgaaggttcc cgcggatgtg ttgaccgtgg acacaccgtt gaaggtattg aacgtggcca
      901 ccacactcga ttggttactg ccggagatcg ttccgttcaa gacaccagtg atcatgaccg
      961 gactcgacgt gctcggggtc tccccggcga ccgtggcggg gataaagcta atgctaaatg
     1021 gggtgttggt ataagtcgtt gattggccgg tgagctcgga ggcgacctgg aaagaaccga
     1081 gtggcacgtt ggaagtagcg ctgacctgag aatttgtgat tggcacaaag ctgatcacat
     1141 tcgtaccact gactccgtcc cctgcttttg tcgtgtctac ggatccggcg gtgctgtaac
     1201 cgaggaccgc atcggaactg gtggccgaga tcgcagcagc attgacgcct gtttgtgcac
     1261 aggcgccgag cccgagggcg acgaccgcgg ttcgcagccc cgtccaccct agtgtcatac
     1321 gcatcatttc ctcctgaaac ggatgggata gtcgcgatcc gagccgcgac tccgcaaagt
     1381 cttagtctgg actcgaagtg aggtgtcctc cccaggacca atatgctctg gagcttccca
     1441 ccggccagac cggcacggga cgactcttgg cgcgaggcgg acgaacgaca cggatggcgg
     1501 cactcacgcc agccaaggca ccggaccgca atccggttga acccttttct acgcgacctt
     1561 ttgatcagga aatgaagttc acggaggggt cgatcaaaat ctcagcctcc atgcccaacc
     1621 tgaccggaac tcaaccatga attccgttgt cgcatagtcc ggtgtgaacg aacggctgga
     1681 aacttaaccg gttggtcgaa acttgtaaac ggaactttag agatttttga ccaaattccg
     1741 attttttttc tgcggccctc gccagcaccc actcaaagca tcgtgatggg gtcgacatcg
     1801 atcgccagct cgatgccatg ctgggcggga tgattcctgg gtaccatgtt caccaagttc
     1861 tgaagtggcc tggagttcgc gcagcgcaac tggagatgga atcgaaacag gtttcggatc
     1921 ttgatgatcg gtgccggagc gggcccgact acgcgcacgg agggatcggc cgccaggcga
     1981 aatgcggccg ccaggtcttt catgtaggcg aatactgcct tatcttctgg cccccgcgcg
     2041 atcacacgta ccaagcggcc gaatggcggc acgccgaatt tttcacgctc gggcagctca
     2101 ttctgggcga attcaagaaa ggcgtgtttg gcggccgagc ggatcgcggg atggtccggc
     2161 gcatacgttt gcacaagcac ccgccccggc cgatctcccc ggcccgtgcg gcctgccacc
     2221 tgcgccacaa gctgaaaggt ccgctcggcg gcgcgaaaat caggcaggtg aagcgccgtg
     2281 tcggcgttga ccactcccac caaggtcaca ttgggaaaat ccagaccctt ggcgatcatc
     2341 tgagtgccca gcaatatccg tacaaggccg gctttgaacg cgcccaagac ctcctcgtgg
     2401 ctgcccggag agcgcatcgt gtccgagtcc atccgccgcg cgaccacatc gggaaacgcc
     2461 gcacggatct cgcgctcgag ccgctcggta ccgattcctc catagtgcaa ggctggcgcg
     2521 ccgcatcccg ggcatgccgg cgggcaggcg cgttccgcat cacatgtgtg gcaaatcagg
     2581 gtatgacggc ttttatgata cgtcgccgcc acgtcgcacg catggcactt gacgacatgc
     2641 ccgcaccgcg gacagagcac gaatgtgtga aagccgcgcc ggttgaggag aaggatgatt
     2701 tgccccccct ctaggagggc ttgaaccatg gcctggcgga gcgactcgga gagccctgcc
     2761 accgcggcct tttcattgcg catgtcgatg atctcgacac gcggcatagg ccggccgccg
     2821 acgcggctgg gcatcgccag ccgcgtataa cgcccgcgct cggcatttcg ccagctttcg
     2881 agcgaaggcg tagccgaccc taaaagcacc ggcacgcctt ccatctgcgc gcgcatgacc
     2941 gccacgtcgc gcgcgttgta ccgcggcgtc gtctcttgct tgaagctgct ctcgtgctct
     3001 tcgtcaacga ccaggagacc cagccgacgc gccggcgcaa acacggccga ccgagcgccc
     3061 acgacgacct gaacctcgcc cgaggcgatg ctctgccagt gccgatgccg ctcggcgtca
     3121 ctcatgtggc tgtgcaagac cgcgacgctg gcaaatcgtc gccggaaccg gcggatcgtt
     3181 tgcggtgtca ggctaatctc aggcaccatc acgatggcct cgcggccccg tgccaccact
     3241 tcctcgatcg ccgccaagta cacctcggtt ttgccgctgc cggtgacccc atgaatcagg
     3301 aaaggaccaa atccgccgga ctcgatcgcc ggcaccagat ggtccagagt caccgcctgc
     3361 tcagccgtca agacgggtcg cggcagatcc ttggcggttt ttcctttttg cgatggcgcg
     3421 tcagtgtcat caagggccgg cggtgcatcg tccggactga tcgttttcga cagtccaacg
     3481 ggcaaccgtc gtttgaccga gccgaccaac ccctcttttc gcagcgcctg gatcggcacg
     3541 ggcgtgcatt tggccatgcg gcagacgtcc acgactgtca acggctcgtc actcctacag
     3601 agcacttcca gcaccgccgt ctgctttgga ggcagtcgcc gcttgaggat ttcctcgcgc
     3661 aaatcggccc ggatctccgc aggcaccagc agaaaggtcc cgatccgcgt cccagcgtgt
     3721 tttttgaccc ccgccggcac cacggcatcg agtgcctggc cccaggaaca cgcataatag
     3781 tccgcgatcc agcgggtcaa ctggagcatc gtgccgtcga tcaaggggag cgggtcgaga
     3841 acctcaacga cctctttgat ccgcgccgga tcgagatcgg caggagccga tgcgtccacc
     3901 cgcacgacgt agccgaccgc cggtttggtg cctcgcccca gcggaacgcg aaccctctgg
     3961 cccgcgcgga tgacactctt gagccgactc gggacccgat aggtcaacac ttgctcgatc
     4021 ggccgattga aaaccacacc gacaaacgga ccctcggcga gcgtctggtc tgcgtccgac
     4081 atcccgaacc acgcgctctg tcgcggcgag acgactgcgc tccgctcgcc ttccgccccc
     4141 tggtccgagc cggcacgggc ggccgtgccg tcgtcgagct caccatcggc tttcgcttgt
     4201 aacacgttca tggacggggc ctgttccccg aggtcgtttt ccagcttcgc atcattatat
     4261 cggatcctgg ccaagatctg ggtcctcgcc agtaagctgc ttcggaggtc gccggcagta
     4321 acccgtttct gccaggaagg gccggacggc gcgcgcgcac tcaccgtcgg cgaaattcgg
     4381 ct
//
LOCUS       DATAQL010000968         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1015420, whole genome shotgun sequence.
ACCESSION   DATAQL010000968 DATAQL010000000
VERSION     DATAQL010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015420"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VHS97_16805"
     CDS             <1..1185
                     /locus_tag="VHS97_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449917.1"
                     /translation="LGLYLDAMFLVTLTALVPTGLYAWLRMGRSRAGLGLAGVFFVAM
                     LVGLIPREIGRRVDPSDAYPSQFQTTLKRQAILEHSRILGLYCLPRLIAGTELTHLEN
                     AGVTIYTLGSRVITMRAPRIRAMLPGQAEWLAVLLVGAFLVALFRLARDPARLADTAR
                     TAVSLATLASALLVVAAFLVNRNIFNSDNYRYLIFLLTPWALGFGLWMNDLANKSWAG
                     RLVALIVAGLLFELMTGAAFLWYRDERLYVDQRGYPVRAQGPDWSELTVLKEVPRGSL
                     SDSRDFVVPSDVTHVFGGYWDVYRMAFLSGGRVTAIPFPSYPNRFRGWSRGLGPGRGK
                     LLVLYPATASRKLGSRSAVDPGAGVVQSAQGTNWPAALATSWVEDGRDPAELRRLRAV
                     VP"
     gene            1209..3779
                     /locus_tag="VHS97_16810"
     CDS             1209..3779
                     /locus_tag="VHS97_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX3449918.1"
                     /translation="MSNGETEAAGCQNGLFERSGWCRWDLVALSVWTAAVAVFFGDLL
                     SLRQALFYFDITEINYPYRAFFAEELRAGRFSRWCPGLYCGLPLYSESQAGYLHPLKY
                     VLYPWMQTWQAFNLDTVLSIWLTGLATYGWLRRHFGPAAALTGAAVFGASGYVWGHLI
                     HTSMINALASVPFVVWGLEASWTSGRWRGVVLGGVALAIQVFAGHLQDSLLTIGLVLV
                     YGLYRAATENRLAARVRALGMAVVLAGLGVLVSGVQWVPSKELLDRSPRAGGMTRGDL
                     TLGSWSPELLPTLVVREAYGTRARDTDWMDGFYPYHEMNTYMGLIAIVLAVIGAGGTA
                     ARDRWVSFWVILIGIGCLLMLGRFTLLFDHAHRLPVIGSSREPVRFHLWVSLGVAALA
                     ACGVERLGRPGTVSLKSGLVLAGVLVAVSIPIMIYIYAVVWTQPKEWTEAYHLARYRW
                     LGRELMLGIARTVFLAVTAWFVAWRAARATDRARRAHRAAMLPLLVLADLFSAHWVDV
                     PTVDPSYWTVPPASALHLKDSPEFIRVFGIGDKASGEPGYASETVDFRAVRDPLDWSL
                     PLVWHLNAAKGNTPMISSRVADFTNQKLGKKLGKTRFDLEGDSHIVTGRRRAGDFPGL
                     RRTRAGAAFIHRNRDALPRVRLMGQPVYADNQPQAVAALERIGRGLRDQLVVEDPTRP
                     LAADAAVSGTARIALDLPERVVIEAQADTPAYLVLADTFDPGWSATVDGRTAPIRPAY
                     VAFRAVYLTPGTHRIVFTYRPAGLEMGLNLTGCGLLLGLLLWFWPSSSLMLAPEHTVL
                     FWPPRWRTWWFITLAAIVVVSAVAFGPGGRPRLHSRWKNSLHRHTWGAGLAANRANRM
                     "
BASE COUNT          592 a         1221 c         1264 g          837 t
ORIGIN      
        1 ctcgggctct atctcgacgc gatgttcttg gtgacgctca ccgcccttgt acccaccggc
       61 ttatacgcct ggttaagaat gggacgctcg agagctgggc tggggctggc cggcgtgttt
      121 tttgttgcca tgttggtcgg cttgatcccg cgtgaaattg gccggcgtgt cgatcccagc
      181 gatgcgtatc cctcacagtt tcagacgacg ctcaagcgcc aggcgatcct ggagcatagc
      241 cggatactcg ggctctattg cctgccgagg ctgatcgccg gtaccgagct gacccacctg
      301 gaaaacgccg gtgtcacaat ctacaccctc ggcagccggg taattaccat gcgcgcaccc
      361 cggatccgcg ccatgcttcc ggggcaggcg gaatggctgg cggtgctttt ggtaggggca
      421 ttcttggtgg cgcttttccg tctggcgcgg gatccggccc gcctcgccga caccgcccgg
      481 accgccgtca gcctcgctac gctggcctcg gcgctcctgg tcgttgcggc atttcttgta
      541 aaccgcaaca tttttaattc ggataactat cgttacctga tcttcttgct cacaccctgg
      601 gcactcgggt tcgggctctg gatgaacgac ctggcaaaca agagctgggc cggccggctg
      661 gttgcgttga tcgttgccgg acttcttttc gagttgatga ctggggcggc gtttctctgg
      721 tatcgtgacg agcgtcttta cgtcgaccag cggggctatc cggttcgtgc acagggtccg
      781 gattggtcgg agctgaccgt tttaaaagag gtgccccgtg ggtcgctgtc cgactcaagg
      841 gacttcgtgg ttccgtcaga cgtgacccac gtgttcggag gatactggga cgtttaccgc
      901 atggccttcc tctccggcgg gcgggtgacc gccatcccgt ttccctcgta tcccaatcgg
      961 ttccgcgggt ggtctcgcgg gctggggccg ggtcgaggaa aactgttggt cttgtatccg
     1021 gcaactgctt cgaggaagtt gggatcgagg tccgccgtgg atcctggggc aggcgtggtt
     1081 caatcggcgc agggaacaaa ctggccggcg gccctggcga cgtcctgggt agaggacggg
     1141 cgcgatccgg ccgagctacg tcgtctgcgg gccgtagttc cgtgagcaaa gccagcgagg
     1201 cgcaagtcgt gtccaacgga gaaaccgaag cggccggatg tcagaacggc ctgttcgagc
     1261 gatcgggctg gtgccggtgg gacctggtcg cgctctcggt ttggaccgcc gcggtcgccg
     1321 ttttttttgg ggacttgctc agcctgcgcc aggccctttt ttacttcgac atcaccgaga
     1381 tcaactaccc ttatcgcgcc tttttcgccg aagagctgcg tgccggacga ttctcgcgct
     1441 ggtgcccggg gctttattgt ggcttgccgc tatatagcga gagccaggcc ggctacctcc
     1501 atccactcaa gtatgtgctc tatccctgga tgcagacctg gcaagcattc aacctggaca
     1561 cagtgctgtc gatctggttg accggcttgg cgacctacgg ctggctgcgc cgccatttcg
     1621 gaccggcggc ggcgctcacc ggcgccgccg tcttcggcgc gagcggatac gtctggggcc
     1681 acctgatcca caccagcatg atcaacgccc tggccagcgt tcccttcgtc gtttggggcc
     1741 tcgaggcgtc gtggacgtcg ggtcgctggc gcggcgttgt tttgggcggc gtcgccctgg
     1801 caattcaggt attcgccggc cacctgcagg actcgctgtt gacgatcggc ctcgttctcg
     1861 tctacggact ctaccgcgcg gccaccgaaa accgtctggc tgcgcgcgtg cgcgcgctgg
     1921 gaatggcggt cgttcttgcg gggttgggcg tcttggtctc gggggtccag tgggtgccat
     1981 cgaaagagct gcttgatcgg tcgccgcgcg ccggcgggat gacgcgcggc gatttgactc
     2041 tgggatcgtg gagcccagag ctattaccaa ctctggtcgt gcgcgaagcc tatggcacgc
     2101 gcgcccgaga caccgactgg atggacggct tttatccata ccatgagatg aatacctaca
     2161 tggggttgat cgccatcgtt ctggcggtaa ttggcgctgg cgggacagcc gcgcgcgatc
     2221 ggtgggtaag cttctgggtc atcctgatcg gcatcggatg tctgctgatg ctgggaagat
     2281 ttactttact gttcgaccat gcgcatcggc tgcccgtgat cggcagttcg cgtgagccgg
     2341 tgcggttcca cctgtgggtt tcgttgggtg tggcggcgct ggcggcctgc ggcgtcgagc
     2401 ggctcgggcg gcccggcacc gtctcgctca aaagcggtct ggtgctggcc ggtgtccttg
     2461 ttgcagtctc gatcccgatc atgatctata tctacgccgt ggtctggacc cagcccaagg
     2521 agtggactga ggcctaccac cttgcgagat accgctggct gggccgcgag ctcatgttgg
     2581 gcatcgcgag gactgtgttc ctggcggtca cggcctggtt tgttgcatgg cgagccgccc
     2641 gtgcgactga ccgggcccgc agggcgcacc gagccgcgat gctcccactt ttggtcctgg
     2701 ccgatctttt cagcgcgcat tgggtcgacg ttccaaccgt cgatcccagt tactggacgg
     2761 tgccaccggc atcggccctg cacttgaaag acagccccga gtttatccgc gtcttcggca
     2821 tcggcgacaa ggcttccggc gaacccggtt acgcctccga gactgtcgac tttcgcgcgg
     2881 tccgcgaccc attggactgg agcctgccgc tcgtctggca tctgaatgcc gccaagggca
     2941 acacaccgat gatttcgagc cgagtcgccg atttcacgaa tcaaaagcta gggaaaaagc
     3001 taggaaagac gcgattcgat ctcgaaggcg actcacacat cgtcaccggc cgcaggcgtg
     3061 cgggagattt ccccgggcta cgccggacgc gcgctggcgc cgcgttcatc caccgtaatc
     3121 gcgatgcgct gcctcgcgtt cggctcatgg gtcagcctgt ctacgccgac aaccagccgc
     3181 aggccgtcgc ggccctggaa cggatcgggc gagggctccg cgaccagctt gtggtcgaag
     3241 atcccacgcg cccgctggct gccgatgccg ctgtcagcgg cactgcccgc atcgccctag
     3301 acctccctga gcgtgtcgtg atcgaagccc aggccgacac gcccgcctat ctggtgctgg
     3361 ccgacacgtt cgacccgggc tggtcggcaa ccgtcgacgg ccgtaccgcg ccgatccggc
     3421 ccgcctacgt cgcctttcga gccgtctatc tcacgcccgg tacgcacaga atcgtcttca
     3481 cctaccgtcc ggcgggcctc gaaatgggtt tgaacctgac cggttgcggg ctcttgcttg
     3541 ggctgttgct ctggttctgg ccttcatcgt cgctcatgct tgcgcccgag cacacggtct
     3601 tattctggcc gccgcggtgg cgcacgtggt ggttcattac gctggctgcg atcgtggttg
     3661 tttcggccgt ggccttcggc cctggcggcc ggcccagact gcacagccgt tggaaaaata
     3721 gccttcatcg ccacacctgg ggagccgggc tggcggcgaa cagagccaat cggatgtgaa
     3781 ggcagaatga cccacacccc gtttcaccaa ttcctgaccg agctcttgaa agaggggaag
     3841 atcgtgttcc ggtcggccaa ggcgccgcac gatcggatct cacagccggc tgtggcgttc
     3901 ctcaccgagg catt
//
LOCUS       DATAQL010000969         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_860563, whole genome shotgun sequence.
ACCESSION   DATAQL010000969 DATAQL010000000
VERSION     DATAQL010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860563"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="VHS97_16815"
     CDS             <1..1669
                     /locus_tag="VHS97_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3449919.1"
                     /translation="LSWVLRHRIITLAVMVGLFIYTLVLIPHLGGEFMPPLEEGYLWI
                     RATLPRTISLESAAKMAPQMREVVASIPEVSRVISQLGRPDDGTDVTGFFNLEMGAPL
                     KPMEEWRKKPFYMFGYRLWDQTISREDIEKELMEKFKQYPGINFNFSQYIRDNVEEAL
                     SGVKGANSVKIIGSDLHTLESLGQKVANILRGIRGIENVGVFHIVGQPNLEIEIDRQL
                     CARHGVNVADVEKVVQAAIGGEAFTQMVEGEKLFDITLRLPHVLRDDPEVINRILVDV
                     PGGSDDSPGYRIPLSQLTHMVAHRAGASYVYRENNRRFIPIKFSVRDRDLASAITEAI
                     EKVKDPKNAVTLPEGYDLEWAGEFEQMKDANERLMYIIPISLGLIMMLLYSMFNSVKD
                     CLLVMVNVLEAAMGGVFALYLTGTHFSISGAVGFVSVFGVAVQDGVLLVTYCNQMRAR
                     GYGVWETCLRGPELRVRPVVMTSLTAALGLFPAAIATSIGSQAQKPLAIVVVGAMLCT
                     LFLTRYMMPILYSYFPAPRGHVEGEDQHLVEGTNYAARFMHDFPEDH"
     gene            1679..>2944
                     /locus_tag="VHS97_16820"
     CDS             1679..>2944
                     /locus_tag="VHS97_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449920.1"
                     /translation="MPFKVLRRIGGVLVVGISLGAVGAPAQQAVDAPSHLLSVELPLR
                     PGIPLDMTVPAPSELRLRSFLISVADPGQIAQGSSIEVRVGPASKPATVDAVASKILH
                     VGDPDVLWSILQPKDVAFTVKITKLPAVDPDGSAVKPLRVAVRVAELDRATTAVQGLV
                     ADAAVVDHIAFEAEPNDQPEQANTLNLGETVYGLADDRPYLPLGTELTEKERTAGADW
                     FRFTFGGDVPQLAFFGLDFVDRDVPPDLRIYRKDNGQLVEYTRGIDPQSLQRERPPRQ
                     GANKFTTRVLTKGTYYLWVDACQPDYQLRTKLFDVPPYLKSEQAEKVDPAAITAAARR
                     AIRTAMDFQLLAGESWHANTPRKGHPMDRVANVHHETSTCIACHPTHFTTQSAMAAVK
                     AGYGIEQPFALEFLTERLANNPVPFHGHPD"
BASE COUNT          584 a          924 c          837 g          599 t
ORIGIN      
        1 cttgagctgg gttcttcgcc accggatcat cacactcgcg gtcatggtcg gactcttcat
       61 atacacactt gtcctgatcc cgcaccttgg gggcgagttc atgccgccgc tcgaggaagg
      121 ttatctctgg atcagggcga ctctgccacg gaccatttcg ctcgaaagcg cggccaagat
      181 ggctccccaa atgcgggagg tagtcgcctc gattcccgag gtaagccggg tgatctcgca
      241 gctcggccgt cctgacgacg gtaccgacgt caccggcttt ttcaatcttg agatgggtgc
      301 gccgttgaaa ccgatggaag agtggcgcaa aaagccgttc tatatgttcg gctatcgtct
      361 ttgggatcaa acgatcagcc gcgaggatat cgaaaaagag ctgatggaaa aattcaagca
      421 atatcccggg atcaatttca acttctcaca gtacattcgc gacaacgtcg aggaagctct
      481 ctcaggcgtc aagggggcca actcggtcaa gatcatcggc tcagatctgc acactcttga
      541 gagcttgggc cagaaggtcg ccaacatctt gaggggaatc agggggatcg aaaacgtcgg
      601 cgtctttcac atcgtcggcc agcccaacct cgagatcgag atcgacaggc aactctgtgc
      661 tcgccacggc gtcaacgtgg ctgatgtcga aaaggtcgtg caggctgcca ttggcggcga
      721 agcattcacg cagatggtgg aaggcgaaaa gctgttcgac atcacgctga gactcccgca
      781 cgtcctgcgt gacgatcccg aggtcatcaa ccgtatcttg gtcgacgtgc cgggcgggtc
      841 tgacgatagc cccggttacc gcattcctct ctcacagttg acccatatgg tcgcccacag
      901 ggccggggcc tcgtacgtct accgcgagaa caaccgccgg ttcatcccga tcaagttcag
      961 cgtccgcgac cgcgacctcg cctcggccat caccgaggcc atcgaaaagg ttaaggatcc
     1021 gaaaaacgct gtgactctgc ccgagggcta cgacctcgaa tgggcgggcg aattcgagca
     1081 gatgaaggat gccaacgaac gcctcatgta cattatcccg atctcgctgg ggttgatcat
     1141 gatgctcctt tactcgatgt ttaactcggt caaggactgt ctgctcgtca tggtcaacgt
     1201 gctcgaggcg gccatgggag gggtgttcgc gctctacctt acgggtactc acttcagcat
     1261 ctcgggggcc gtcggctttg tgtcggtctt cggtgtcgct gttcaagacg gcgttctctt
     1321 ggtcacttat tgcaatcaga tgcgagcgcg aggctatggg gtgtgggaga cctgcttgcg
     1381 cggacccgaa ttgcgcgtgc ggccggtcgt catgacctcg ttgaccgcgg cattagggct
     1441 cttccccgcg gcgatcgcca cctcgatcgg atctcaggcg cagaaacctc tggccatcgt
     1501 cgtcgtcggt gccatgctct gcacgctctt cctcactcga tacatgatgc cgatcctgta
     1561 cagctatttc ccagctccgc gcggtcacgt cgagggggaa gaccagcatc tggtcgaagg
     1621 cacgaattac gccgctcggt ttatgcatga ttttccggaa gatcactgag ggacaacgat
     1681 gcctttcaag gtgcttagac gaatcggtgg cgtgctggtc gtcgggatca gcctgggagc
     1741 cgttggagca cccgcccagc aagcggtcga tgctccgtct catcttctga gcgtcgaact
     1801 cccgctgcgg ccgggaattc cgctcgacat gacagtaccc gctccctcag aacttcggct
     1861 gcggtctttc ttgatttctg tcgcggaccc cggtcaaatc gcccagggtt cgtcgatcga
     1921 ggtgcgggtc ggaccagcca gtaagcccgc aaccgttgat gccgtcgcga gcaagatcct
     1981 ccacgtcggc gatcccgacg tgctctggtc gatcctacaa cccaaggacg ttgcgtttac
     2041 ggtcaagatt accaagctac ccgccgtgga tcccgatggc tccgctgtca aaccgcttcg
     2101 tgtcgcggtt cgcgttgccg agctcgaccg agcgactacc gccgtgcaag ggctcgtcgc
     2161 cgatgccgcg gtcgttgacc acatcgcctt tgaggccgag cccaacgacc aacccgagca
     2221 ggccaacacg ctcaacctcg gggagacggt ttacggcttg gccgatgatc ggccgtattt
     2281 gccgctcggt accgaactca ccgaaaaaga gcgaaccgct ggcgccgact ggttccggtt
     2341 tacgtttggc ggtgatgttc cccaactcgc cttcttcggg ctcgacttcg tcgaccgcga
     2401 cgtaccgccc gacctgcgaa tctaccgcaa agacaacggt cagcttgtcg agtacacccg
     2461 cggaatcgac cctcagagcc tccagcgcga gcggccgccg cgccaagggg ccaacaagtt
     2521 cacgacccgc gtgctcacca agggaaccta ttatctctgg gttgatgcct gccagccgga
     2581 ttatcagctt cgaaccaagc tcttcgacgt gccaccttat ctgaagtccg aacaagccga
     2641 aaaggtcgac cccgcggcta tcaccgcggc cgcccggcgc gccattcgca ctgcgatgga
     2701 tttccagctt ttggcgggcg agagctggca cgccaatacg ccgcgcaaag ggcacccgat
     2761 ggatcgagtc gccaacgttc accacgaaac atcgacgtgc atcgcctgcc atccgaccca
     2821 cttcaccacc cagtcggcca tggcagccgt caaggccggc tacgggatcg agcaaccttt
     2881 cgcactcgag tttcttaccg agcggctagc caacaacccg gtcccgttcc acggtcaccc
     2941 cgat
//
LOCUS       DATAQL010000970         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2695938, whole genome shotgun sequence.
ACCESSION   DATAQL010000970 DATAQL010000000
VERSION     DATAQL010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695938"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(694..>1980)
                     /locus_tag="VHS97_16825"
     CDS             complement(694..>1980)
                     /locus_tag="VHS97_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449921.1"
                     /translation="RLWVTVRLAWRFGHSFKRNKFGGSVLWSSERQDQFKASFRRLIL
                     DYWNECYPIEIRHKPTGTRQTVIPQFLLLDVPDGGVDQHMKIWVSALPIGQSYVGQRC
                     KLHAYDAGDDPSHFVLRRIHAKFIRRSVVKTMGQAIQDLAKGGVKFARDHVVLDPQGC
                     NQLDRFAESLHVDLPIFPALEIKVKSHRLPDENPALDGKRVDAVRKYLMGKGFPIKDL
                     VEKPGGTVSGPLLSRVRGVAAALPYVTLQYRQSNTQRFIKSSHIYPIAAHEFGHILGL
                     PDEYHQSARLQNADQANLNEYTSLCAKFQVTKPALNTYSMSLMAAGNQLLPAHYVTLA
                     NAVHQHLMIFYKQVNLNNRLRRGRPTNASPPTAQELQVAFNNMQAQKPWDDYAITIGQ
                     MRRTTTAGMVPLQRYIPLEAALSLPADIKGSGSFGL"
BASE COUNT          336 a          592 c          658 g          394 t
ORIGIN      
        1 aagcattttc cctcgttccc acgctcccgc gtgggaacgt ttcataggac gctccgcgtc
       61 gttccgtaga gccgacggcg aagcgcagcc aagcgacgcc gagcgtcgcg gaccgatgtt
      121 cccacgcggg agcgtgggaa caagccatcg gtcgccccga gcgaaatgct taacggcgat
      181 gggtgagggg tggatcactc ggcgcgtcgc cgggcccgtg gaacgagatg aagcgagcaa
      241 ccggcccact tccggtggga gagcggggcc cgtcgccgcc gtcgtgcggc gccggccgag
      301 gagaccgtcc ctgcgggccg tcgatcccgc gcggccttcg cgtcgtagcc tcccgaaatt
      361 tccagaaacc ttcgtttatg ttaccggccg ttcgggtgga tccgatgacc gactcctcga
      421 cgtcgagaga gagattgccc ccgctcccat tcgatcgacg tccgatatgg ggcggcgagt
      481 cgtcatcgcg gccgccgaca tcctcttctc cggataggcg acgtggccgg gccggcaact
      541 cggatcggag ggcgcggtcg atgatcggct tcttggcgtg atcgcgtggg cgttccgcga
      601 tcgaccgccg ccgcttgtcc ccatgcgctt gcaagcgccg gcggccggga cggcgagatg
      661 ccaagtccac cagaccacct ctcgtcctgt cccctacagg ccgaatgacc ccgaaccttt
      721 gatatcagcc ggtagcgaca atgcggcctc cagggggatg tagcgttgga ggggcaccat
      781 ccccgccgta gtggtgcgcc gcatctggcc gatggtgatc gcgtagtcat cccagggttt
      841 ctgtgcttgc atattgttga aagcgacctg gagctcttga gcggtcggcg gactggcatt
      901 agtggggcgg ccgcgtctga ggcgattatt gagatttact tgtttgtaga agatcatgag
      961 gtgttgatgc acggcattgg ccagcgtgac ataatgggcg gggagcagct ggttgccggc
     1021 cgccatcagg ctcatcgaat aagtgttgag cgccggttta gtgacttgga atttggcaca
     1081 gagactggtg tactcgttca aattcgcctg atcagcgttc tgtagcctcg ccgattgatg
     1141 gtactcgtcc ggcaggccga gtatgtgtcc gaactcgtgg gcggcgatcg ggtagatgtg
     1201 gctgctcttg atgaatcgct gcgtattcga ctgccggtac tgcagcgtga cgtaggggag
     1261 cgccgcggcg acgccccgga cccgcgacag caacggcccg gaaaccgtgc cgcccggctt
     1321 ctcgacgaga tccttgatcg ggaagccttt gcccatcagg tacttcctca ccgcgtcgac
     1381 gcgcttcccg tcgagggcgg ggttctcgtc cgggaggcgg tgggatttga ccttgatttc
     1441 gagcgcgggg aagatcggta ggtcgacatg cagagactct gcgaagcggt cgagttgatt
     1501 acacccctgg gggtcgagga cgacgtggtc cctggcgaac ttgaccccgc ccttggccag
     1561 gtcctgtatg gcttgaccca tggttttcac gacggaccgc ctgataaact tggcatgaat
     1621 gcggcgcaag acgaaatggc tggggtcgtc gccggcgtcg taggcatgca gcttgcatcg
     1681 ctggccaacg tagctttgcc cgatcggcag ggccgacacc cagatcttca tgtgttgatc
     1741 cacgccgccg tccggcacgt ccaggagtag gaattgcggg atgacggttt gcctcgtgcc
     1801 cgtcggcttg tgccggatct cgatcgggta gcactcgttc cagtaatcca ggatgagcct
     1861 gcggaagcta gccttgaatt ggtcctgccg ctccgaggac cagaggacgc tgccgccaaa
     1921 cttgttccgc ttgaaggagt gcccgaacct ccaggccagc ctcacggtga cccagaggcg
//
LOCUS       DATAQL010000971         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3763815, whole genome shotgun sequence.
ACCESSION   DATAQL010000971 DATAQL010000000
VERSION     DATAQL010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763815"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1337
                     /locus_tag="VHS97_16830"
     CDS             105..1337
                     /locus_tag="VHS97_16830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HEX3449922.1"
                     /translation="MKSRRIWHSLASLQSMLTVLLLAGSAAAGTTQIYSFTGGNDGEY
                     TDTELVMDRAGNLYGTSVQGGMFGGGTVFQLSPTGGTWIHTVLYDFTGGPDGGEPYKG
                     VTLDAHGNLYGTAVTGGGGSCEGGCGVVYKLTNSGGVWTQTVLHAFTGGEDGSGPGSP
                     VAFDQHGNLFGTTPTAGVNGYGTVYEMKPGAGGTWGLTVIHAFTGGIDGLGGSASRLL
                     VDAVGNLYGVNTVGGANGFGNVFKLSSSGGKWQLTTLYSFWDQPDGASPYGGVVFDKS
                     GNLYGTTYYAGANDVGTVYELTNHNGSWTETVLYSFKGGSDGDSPISTLVIDARGNLY
                     GTTSDGGAAACGCGTIFRLSPSTGGVWTESVVYRFPGSPKPGFAYNGMVSDGKGSFYG
                     ATVHGGSGNDGAIYKFTP"
     gene            complement(1352..3163)
                     /locus_tag="VHS97_16835"
     CDS             complement(1352..3163)
                     /locus_tag="VHS97_16835"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3449923.1"
                     /translation="MSKSRREFLTQTSLSLLAAATVYRSYAQQPDAASQSSLPPGAPP
                     AFGVGPAVGPEVSPITFAEAAKLVQVEMTEGEREMAAGSWRKTMAALYERRTGPRTVV
                     LPSTLAPATKWNPALPGLKVGPERDRFVRSNVDPGPLPARDEDIAFASVTQLSRWIEH
                     RKLSSDRLTRIYLKRIEQFDPKLRCVITLTRDLALAQAKKADEEIAVGKYRGPLHGIP
                     WGGKDLLDTAGIRTTYGAEPFQNRVPAEDAAVVKRVHEAGAVLVAKLSLGALALNDVW
                     FGGQTMNPWLLEEGSSGSSAGPGAATAAGLVAFAIGSETGGSIVSPSMRCGLTGLRPT
                     YGRVARTGAMTLCWSLDKLGPMTRSVEDALLVLHAITGPDVGDLASVPSKLDFDAAAK
                     VQGLRVGYFPRWMNENPATAVDRTALETVKKVGMVPVEVSIPNWPYDSLDVILFAEAA
                     AAFEELTLSGKANQLKAQVPDAWPNIFRESRFLSAVDFVQADRFRRMVAEEMARVFSE
                     VDLLLVPSLRDEMLTLTNFTGHPSLTLRAGFVEVAKARTDWAPDPNHPMPTFSPPRRV
                     PHGVTLIGRLFDEGTLGSAGLALERAFGVVNERPPGF"
BASE COUNT          659 a         1021 c          994 g          608 t
ORIGIN      
        1 acctcaacaa aattgcgtgc ctgacggtga ttttcaatgg tatagtgcct tgctgcacaa
       61 gcaatgtcgt cgcgtttcaa ggcattgggc cggaaagggg aacgatgaaa agcagacgaa
      121 tctggcattc actggccagc ctccaatcca tgcttacagt gttattactg gccggttccg
      181 cggccgcggg cacaacccag atctacagct ttaccggcgg taacgacgga gaatataccg
      241 acactgaact ggtgatggac cgcgccggaa atctttatgg cacgtcggtg caaggcggga
      301 tgttcggcgg tggcacggtc ttccagttgt cgcctaccgg cggcacgtgg attcataccg
      361 tcctatacga cttcaccgga ggccccgacg gaggcgagcc ctacaagggc gttaccctcg
      421 atgcccacgg caacctctac ggtacggctg tgacgggcgg cggcggttct tgcgagggcg
      481 gatgcggcgt ggtctacaag ctcaccaact ccggcggcgt gtggacgcaa accgtgctcc
      541 atgctttcac cggcggcgag gatggctctg gtccgggaag tccggtcgcg tttgaccagc
      601 acggcaatct ctttggaacg actccaaccg caggcgtcaa cggttacggt acggtctacg
      661 aaatgaaacc cggcgccggc ggcacgtggg gcctgactgt gattcacgcc ttcacaggag
      721 gcatcgacgg attaggcggg tcagccagcc gtttgctggt cgatgccgta ggcaatctct
      781 atggagtaaa cactgtcggc ggcgccaatg gcttcggcaa cgtgttcaag ctgtcgtcga
      841 gcggcggcaa gtggcaattg accacgcttt attctttctg ggatcagccg gacggagcct
      901 ctccctacgg aggagtcgtt ttcgacaaga gtggaaacct ctacggcacg acgtactatg
      961 ccggcgcaaa cgatgtgggc accgtctacg agctgacgaa ccacaacggg tcctggactg
     1021 agaccgtgct ctacagtttt aaaggcggaa gcgacggaga cagccctatc agcactctgg
     1081 tcatcgatgc gcgaggcaat ctgtacggga ccaccagcga cggcggcgct gcggcatgcg
     1141 gttgcggaac cattttccgg ctcagcccct cgactggagg cgtttggacg gaaagcgtcg
     1201 tctatcgttt ccccggctct ccgaagccgg gattcgcgta taacggaatg gtctccgacg
     1261 gaaaaggaag tttctacggc gcgaccgtcc acggcggaag cgggaacgat ggcgccatct
     1321 acaagttcac tccgtaagaa gagtccgcga cctagaagcc cggcggccgc tcattcacca
     1381 cgccaaacgc ccgctccagc gcgagccccg cgctgcccaa cgttccctcg tcgaaaagcc
     1441 ttccgatcaa tgtgactcca tggggaacac ggcgaggcgg cgaaaacgtc ggcatggggt
     1501 ggttcggatc gggcgcccag tcggtccgcg cttttgccac ttcgacgaaa cctgcgcgca
     1561 gcgtgagcga aggatgcccg gtaaaattgg taagcgtaag catctcgtcg cgcagcgaag
     1621 gcaccagcaa caaatctact tcggagaaga cgcgcgccat ctcctcggct accatgcgac
     1681 ggaatcgatc ggcctgcacg aagtccaccg cggaaagaaa cctcgactcg cgaaaaatgt
     1741 tcggccacgc gtccggcact tgcgccttaa gttggttcgc cttgccgctt agcgtgagtt
     1801 cctcgaacgc cgctgccgct tccgcgaaaa gaatcacgtc gagcgagtca tacggccagt
     1861 tgggaatcga aacttcgaca gggaccattc ccaccttctt cactgtttca agagcggtgc
     1921 ggtccactgc cgttgcggga ttttcgttca tccagcgcgg aaagtagccg acgcgcagac
     1981 cctgcacctt cgcagctgca tcgaaatcga gcttgctggg cacgctggcc agatcgccaa
     2041 cgtctggccc ggtgatggca tgcagcacca gcaacgcgtc ctcgacactg cgcgtcatgg
     2101 ggcccagttt gtcgagcgac cagcacagcg tcatcgcgcc ggtgcgcgcc acccggccat
     2161 aagtaggacg aagcccggtg agtccgcagc gcatcgaggg actaacaata ctgccgcccg
     2221 tctcactgcc aattgcgaat gccaccaacc ccgcggcagt agcggctcca gggccggcgc
     2281 tcgaacctga ggaaccctcc tccagcagcc acggattcat agtttgtccg ccgaaccaga
     2341 catcgttcaa ggcaagcgcg cccaggctta atttggccac gagcactgcg cccgcttcat
     2401 gcacacgctt caccaccgcg gcgtcttcgg cgggcacgcg gttctgaaaa ggttcggcgc
     2461 cgtaagtggt gcggattccg gcggtgtcga gcagatcttt gccgccccag ggaatgccgt
     2521 gcagcggccc gcgatatttt ccgacggcga tttcttcgtc ggcttttttc gcttgcgcca
     2581 gcgccaggtc ccgcgtgagc gtgatcacgc agcggagctt gggatcgaac tgctcgatac
     2641 gcttgagata aattcgcgtg agccggtctg aactcagctt gcgatgctcg atccaacgcg
     2701 aaagctgagt cacggaagcg aacgcaatat cctcgtcgcg cgctggcaac ggacccgggt
     2761 cgacgttgct gcggacgaac ctgtcgcgct ccggaccgac tttcaaccct ggcagcgccg
     2821 gattccactt cgtagccggg gccagcgtcg atggcagcac aactgtacgt ggcccagtcc
     2881 gccgctcgta aagcgccgcc atggtcttgc gccaactacc tgccgccatc tcacgctcgc
     2941 cctcggtcat ctctacctgc accagttttg cggcctcggc gaacgtgata ggcgaaacct
     3001 ccggccccac ggccggcccc acaccaaacg ccggaggtgc gcccggaggc agactgctct
     3061 gggaagcagc gtccggctgc tgagcatagc tccggtagac cgtggcagca gccagcaggc
     3121 tgagtgatgt ttgcgtcaag aactccctgc gcgatttcga catggttcct ccgacaggca
     3181 ttgtagcggc tgggcggtac ggtaagggtt caaaaccctt ggcaggggat ggtgcgcctc
     3241 tgagcgggag tgtactatgg ccagcatccc ggaggccaca gg
//
LOCUS       DATAQL010000972         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1623778, whole genome shotgun sequence.
ACCESSION   DATAQL010000972 DATAQL010000000
VERSION     DATAQL010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623778"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VHS97_16840"
     CDS             complement(<1..1233)
                     /locus_tag="VHS97_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX3449924.1"
                     /translation="MTERSRWCGSLSFSWNPPIFAMSHVGKLLIETITHTDPAIRDRS
                     MRELVAGSSLDEKIEACAQIEDFRRNCQNLYERVRASLFLSALYRFEIQDAVGIRQSG
                     LIPFSGFMDLMERRYESAIASFLETMKMSGPDGTIASALARAYEQSAFQTLADQVRRS
                     VRSCQGNRWMFRVGGADEHPLWIHPRLLLRESEESLFPILVEKTSVRLDLSHSGWSDI
                     FFLGMDFPEGARVLNISVDLGVHGRDHAPIPPIESRVRVITEPILRLTSIDLNACKDV
                     DSLEELFNFGNDYLGLIKAAVVASGLVPPALEGTHTRLADLLGRVVRPGYGLEIVSKV
                     NDIPKGSRLAVSTNLLASLITLLMRATGQTRNLTGPLDSQEAKVVVARAILGEWLGGS
                     GGGWQDSGGIFPGIKLIEG"
     gene            complement(1230..2186)
                     /locus_tag="VHS97_16845"
     CDS             complement(1230..2186)
                     /locus_tag="VHS97_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX3449925.1"
                     /translation="MQISKAVITAAGRGARQYPASDTVQKVMLPLVDRDGVTKPVLQI
                     IAEEAFESGIEEICVVSAPGDENVYRSHFRNYAANLRLTYKGLDWAEQQARRIVDLEQ
                     RLQFAVQPEPEGYGHAVWCARSFVGGAPFLLLLGDHLYLSAEPRRCARQLIDLASAEN
                     CAVSAVQATREHLIHQYGTLTGRRQPQHPDIYAIDEIIEKPNPTLAELRLHVPGLRAG
                     HYLCFFGMHLLTPVVFDLLDQLVRDNVRELGQIQLTTALNVLARKERYLALETRGSRF
                     NLGVKFGVVEAQIALAMGGVDREEMLALLLESMVRIERNSTR"
BASE COUNT          502 a          781 c          767 g          454 t
ORIGIN      
        1 gccctcgatc agcttgatgc ccggaaagat tccgcccgaa tcttgccagc cgccgcccga
       61 tcctcccagc cactcgccca ggatcgcacg ggccaccacg actttggcct cctgggagtc
      121 gagtgggccc gtcaggttgc gcgtctggcc agtcgcgcgc atcaagagag tgatgagtga
      181 tgccagcaag ttcgtcgaga ccgccagccg tgaacccttg gggatgtcgt tgaccttgct
      241 cacgatctcc agaccgtatc ctggccggac gacgcgcccc agcagatcgg ctagccgcgt
      301 atgagttccc tcgagcgccg gcgggaccaa gcccgaggcg acgacggccg ccttgatcag
      361 gcccaggtag tcgttgccga agttgaatag ctcttccaag ctatcgacgt ccttgcatgc
      421 gttcagatcg atactggtca gtcgcaggat cggctcggtg atcactcgca cccgcgactc
      481 gatgggaggg atgggagcgt ggtcgcggcc gtgaaccccg agatcgaccg atatgttgag
      541 cacccgcgcc ccctcgggga aatccatgcc gaggaagaag atgtcggacc agccgctgtg
      601 cgaaaggtcc agccggaccg atgtcttctc gaccaggatc gggaatagtg attcctccga
      661 ctcgcgcaag agtaatcgag gatggatcca gagtggatgt tcgtcggccc cgccgacgcg
      721 gaacatccag cggttgccct ggcagctccg gaccgagcgg cgcacctggt cggccagggt
      781 ctggaacgcg ctctgctcgt aggcacgcgc gagtgcgctg gcgatcgtgc catcaggccc
      841 actcattttc atcgtctcga gaaagctcgc gatcgccgat tcgtagcggc gctccatcag
      901 atccatgaac ccggaaaaag ggatcaagcc cgactggcga atccccacag cgtcctggat
      961 ctcgaagcgg tagagagcac tgaggaacaa ggaggcgcga acgcgctcgt acaggttctg
     1021 gcagttgcgc cggaaatctt cgatctgcgc gcacgcttcg atcttctcgt cgaggcttga
     1081 gccagccacc agctcacgca tcgaccgatc ccggatcgcc ggatcggtgt gagtaattgt
     1141 ttcgatcaag agcttgccga catgagacat ggcaaaaatc ggagggttcc aggaaaagga
     1201 gagcgacccg caccaccgtg atcgctcggt catctcgtcg aattccgctc gatccggacc
     1261 atggattcga gtaagagcgc cagcatctct tcgcggtcga cgccccccat ggccagggcg
     1321 atctgagcct cgaccacgcc gaacttgacc ccaagattaa accgggagcc ccgggtttcg
     1381 agcgcaagat agcgctcctt ccgcgcgaga acgtttagcg cggtcgtgag ctggatctgg
     1441 cccagctcgc ggacgttgtc gcggacgagc tgatcgagca agtcgaagac aaccggcgtc
     1501 agcaaatgca tgccgaaaaa gcaaaggtag tgccccgcgc ggaggcccgg gacgtgaagc
     1561 cgcagctcgg ccaaggtcgg gttgggtttt tcgatgatct cgtcgatggc gtagatatca
     1621 ggatgctggg gctggcgccg acccgtgagc gttccatact gatggatcag atgttcgcga
     1681 gtggcctgta cagccgagac ggcgcagttt tccgcggagg ccagatcgat caattgtcgt
     1741 gcgcaccgcc tgggctctgc ggagagataa agatggtcgc ccaagagcaa caggaacggc
     1801 gcacccccca caaaagaccg cgcgcaccag accgcgtgac cgtatccctc gggctcgggc
     1861 tggacggcaa attgcagccg ctgctcgaga tcgacgatgc ggcgcgcctg ttgctcggcc
     1921 caatcgagcc ccttgtacgt caagcgcagg ttggcggcgt aattgcgaaa gtggctgcga
     1981 tagacgtttt catcgcccgg cgcgctgacg acgcagattt cctcgatgcc gctctcaaag
     2041 gcttcctcgg cgatgatctg gagaacgggc ttggtgacac cgtcgcggtc gaccaagggc
     2101 agcataactt tttgcacggt gtccgacgcc ggatattgcc gggccccgcg gccggccgcc
     2161 gtgatcaccg ccttcgatat ttgcaccgag gcttatcctc tcgaggtcag ggctctattc
     2221 gaatcgccca ggggactcga acccgcggaa aaagcggttt tccctttcaa acgagaaggt
     2281 tagcctagcc attccactga gcgtccggca acccctgtct cttgacaggt cgcgctctag
     2341 gcgcacaatc aaaactgccc ctcacgggac actgttactt catcccccga tggatctcca
     2401 agtgcaatgg acccgctttc tggcccacaa gcggtcgaca tggcctggaa acgctaccag
     2461 gccggcgact tgccgcaggc ggagcagatt tgccggcaga tcgt
//
LOCUS       DATAQL010000973         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3917412, whole genome shotgun sequence.
ACCESSION   DATAQL010000973 DATAQL010000000
VERSION     DATAQL010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3917412"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2041
                     /locus_tag="VHS97_16850"
     CDS             <1..2041
                     /locus_tag="VHS97_16850"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3449926.1"
                     /translation="PDGKSLVYVSWAPDGGDIWKRSGDGRGEPEKLTRARAFYHDPVW
                     TPDGKRIVALRAPRQSRVENPYGMGAVLDLVWIPAEGGEATLISPARGASRPHFGKEA
                     DRIYLTTPLGLVSMRFDGTDRRTHLAVTGKTSYAPSEPEPAEEIILSPNGRWVLARVS
                     TQLYLLAMPQAGGEPPKVPVHEPSVALKKLTDIGADYTAWADGGKTITWAIGSSFFRL
                     PFDSIIFDPLKTEDEAKGEDKDQIEAEKKQRAPKPEELAVVIERPRRRPRGSVVLSGA
                     TVVTMRGDEVIAQGDIVVTDHRIVAVGPKGSVKAPEGTKVIDMAGTTIVPGFVDTHAH
                     WTNVRHGVLDLQNWCFFANLAYGVTTGRDPQTGTNDMFAYQDLVETGDLVGPRAFSTG
                     PGVFSDNDFQSAEEVEGVVARYKKFYRTNTLKSYMIGNRRQRQWMVEACRKNEVMPTT
                     EGGLDLKLNLTHAIDGFSGNEHSLPVVPLFSDVVDLFARTGISYTPTLLVAYGGPFGE
                     TDFFTTTSIHDDPKIRRFIPHEILDSKVRRSAWFHKSEHIYPLIAESAAKVARAGGHV
                     CIGSHGEMQGIGYHWEMWALASGGWQPLEVLRAATLHGAEAIGYAQDLGSLETGKLAD
                     LVVLSADPLKDIHNTSSVRYIMKNGELFDGDSLDQIWPEKKPLPRLWWWDEKPTK"
     gene            2177..3868
                     /locus_tag="VHS97_16855"
     CDS             2177..3868
                     /locus_tag="VHS97_16855"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3449927.1"
                     /translation="MKSLAFLGVGTSTFRRALAAQAAQAGTVTTEMIKQSEWIAGLEL
                     TQDERTSTARTIVRSLRSFADLRKVDVGYDIPPALTFLPDLPRPAGGIRRNQARPAEL
                     STPRRPDSAEELAFLPLGALSALIKSRQISSTELTKLYLERLRRFDPLLKCVVTYTDD
                     VALKQSAQADEEIAAGHYRGPLHGIPWGAKDLIAYPGYPTTWGATPFKNRVIDEKAAV
                     AARLEDAGAVLVAKLSLGALAMGDLWFGGRTRSPWDPRAGSSGSSAGSASAVAAGLVG
                     FAIGSETLGSIVSPCRACGASGLRPTYGRISRQGCMTLSWSMDKLGPIARSIEDCALV
                     FDAIHGADGRDWAAVDYPFDWPPKLALENIKVGYIEERDRPVDDREELRVLRKIGFEL
                     VPITLPDDLPIQAITLMLGTEAAAVFDEITRKHITEGLNSWPDSFRNGQFVPAVEYLR
                     AARVRTKLMRAMAERMAKVDVYVGSGQDLSITNLTGHPCAVFPMGFRDRDGRLMPGSV
                     TLTGRLYDETTLLAVAHAFQQATGDHYKRPPVEAYLADEQKRIEQEKSKAKEAAG"
     gene            3994..4191
                     /locus_tag="VHS97_16860"
     CDS             3994..4191
                     /locus_tag="VHS97_16860"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX3449928.1"
                     /translation="MKYKIVLEESEEGFAVSAPGLPGCHSQGATEEEALENIKDAIRE
                     YLMSINDRLRTANVREVEVEI"
BASE COUNT          874 a         1331 c         1356 g          781 t
ORIGIN      
        1 gcccgacggt aagtcgctgg tttacgtgag ctgggcgccc gatggagggg acatctggaa
       61 gcgctccggc gacggccggg gcgagcccga aaagttgacg cgggctcgag ccttctacca
      121 cgacccggtc tggacgccgg atggcaagcg catcgtggcc ctgcgcgcac cgcgccagag
      181 ccgcgttgag aatccctatg gcatgggagc cgttctcgac ctcgtctgga tacctgccga
      241 agggggcgaa gccacgctca tcagccccgc tcgaggcgca agccggccgc acttcggtaa
      301 ggaagccgac cgcatctact tgacgacccc tctgggcctt gtatcgatgc gtttcgacgg
      361 caccgaccgt cgtacgcatc tggccgtcac cggcaaaacg agctatgcgc cctccgagcc
      421 ggaaccggcc gaagagatca ttctctcgcc taatggacga tgggtcctgg cccgggtctc
      481 aacccagctt tacctgcttg ccatgcctca ggccggcggc gagccgccca aggttccggt
      541 ccatgagccc tccgttgctt tgaagaagct aaccgacatc ggcgcggact ataccgcctg
      601 ggccgacggt ggcaagacca ttacctgggc gatcgggtcg agctttttcc gcctgccttt
      661 cgattcgatc attttcgatc ccttgaagac cgaagacgag gccaagggcg aggacaaaga
      721 tcaaatcgag gccgaaaaga aacaacgggc tcccaagccg gaggaactgg ccgtggtgat
      781 cgagcgcccc aggcgccgcc cgcgcggctc ggttgtcttg agtggcgcca cggtggtcac
      841 gatgcgcgga gacgaggtca tcgctcaagg ggacatcgtg gtcaccgacc atcgcatcgt
      901 ggctgtcgga cccaagggct cagtcaaggc acccgaggga accaaggtca tcgacatggc
      961 agggacgacc atcgtccctg ggtttgtcga cacccacgca cactggacca atgttcgcca
     1021 cggagtgctc gatctgcaga actggtgctt ttttgcgaac cttgcctatg gcgtgacgac
     1081 gggccgtgat ccgcagaccg gcacgaacga catgttcgca tatcaagacc tggtcgagac
     1141 tggcgacctg gtgggtccgc gcgcgttctc gaccgggccg ggagtttttt cggacaacga
     1201 ttttcagtcg gcagaggaag tcgagggagt cgtcgcccgc tacaagaagt tctaccggac
     1261 caacaccctg aagtcgtaca tgatcggcaa ccgccgccag cgtcagtgga tggtggaggc
     1321 gtgccggaaa aatgaagtca tgccgaccac cgagggaggg cttgacctca agctgaatct
     1381 gacgcacgcg atcgacggct tcagcggtaa tgaacacagc ctaccggtcg ttccgctatt
     1441 cagcgacgtg gtcgatctgt tcgcgaggac gggaatctcg tatacgccaa cgcttctggt
     1501 cgcgtacggc ggcccgttcg gcgagaccga tttcttcacg accacgtcca tacatgacga
     1561 cccgaaaatc cggcggttca tcccgcacga aatcctcgat agcaaggtca ggcgctcggc
     1621 atggtttcac aagagtgagc atatctatcc gctgatcgcc gaatcggccg ccaaggtcgc
     1681 gcgcgcggga ggacacgtct gtatcggcag tcatggagaa atgcagggaa tagggtatca
     1741 ctgggagatg tgggcgctgg caagcggcgg atggcagccg ctcgaagtct tgcgcgcagc
     1801 cacgctgcat ggcgcggagg cgatcgggta tgcgcaggat ctgggcagtc ttgagactgg
     1861 caagctcgcc gacctggtcg ttctgtcggc cgaccctctc aaggatatcc acaatacgag
     1921 ctcagttcgc tatatcatga aaaacggcga acttttcgat ggcgattcgc ttgaccagat
     1981 ctggccggaa aagaagccgc tccctcgcct gtggtggtgg gacgaaaagc cgacgaagta
     2041 gctcatttcc caactcgatc ctgtacaact tttgagcgag atgaccacag tgaccctgaa
     2101 cggcccagac gacacaccga ccgagactgg aacacccgcg ccgaaagtgc gacgcgctgt
     2161 tcgcaggcga acgctcttga aaagcttggc cttcttgggc gtgggcacga gcacatttcg
     2221 gcgcgcactt gcggctcagg ccgcacaggc ggggaccgta accaccgaaa tgatcaagca
     2281 gtcagagtgg attgcgggtc tggagctcac ccaggacgaa cgaacaagca ccgctcggac
     2341 aattgttcgt agcctccgtt cgttcgccga cttgcgcaag gtcgatgtcg gttatgacat
     2401 tcccccggca ttgacattct tgccggacct gccgcgaccg gccggaggga tccgccgcaa
     2461 ccaggctcgg ccggccgaat tgtcaacgcc gcgccgtccc gattctgctg aggagctggc
     2521 gtttttgccg ttgggcgcgc tctccgccct gatcaagagc cggcagatca gctcgacgga
     2581 gctgacgaaa ctctatctcg agcgtctcag gcgatttgac ccgctgctca agtgcgttgt
     2641 gacctacacc gacgacgtgg ccttgaaaca atccgcccag gcggatgaag aaatcgccgc
     2701 gggccattac cgcgggccac tgcatggaat tccctggggt gccaaggacc tgatcgccta
     2761 tcccggctac ccgacgacct ggggggcgac tccattcaag aatcgggtca tcgacgaaaa
     2821 agcggcggtg gcggcgcgtc tcgaagacgc cggcgcggta ctcgtcgcca agctgtcctt
     2881 gggcgcgctg gcgatgggag acctctggtt cgggggccgg acgcgcagtc cctgggatcc
     2941 gcgcgcgggt tccagcggat catccgccgg atcggcctcg gccgtcgccg cgggtctggt
     3001 cggttttgcg atcggcagcg agacgctcgg gagcatcgta tctccttgcc gtgcctgcgg
     3061 cgcctcgggg ctgcgcccga cctacggccg aatcagccgg cagggctgca tgactctctc
     3121 gtggtcgatg gataagctgg ggccgatcgc ccgttcgatc gaggactgtg cgctggtttt
     3181 tgacgccatc catggcgccg acggtcgcga ctgggccgcc gtggattacc cttttgactg
     3241 gccccccaag ctcgccctcg aaaacatcaa ggtcggctac atcgaggagc gcgaccgccc
     3301 cgtggatgac cgtgaagagc taagggtgct gcgcaaaatc ggctttgagc tggtgcctat
     3361 cacgctgccg gatgacctgc ccatccaggc gatcaccctc atgttgggga ccgaggcggc
     3421 cgctgtattc gacgagatca cgcgcaaaca tatcacggag ggtttgaact cctggcctga
     3481 ttcgttccgc aacggccagt tcgtcccggc ggtcgagtat ctgcgagccg cccgggtgcg
     3541 taccaagctc atgagggcca tggccgagcg aatggccaag gtcgacgtct acgtcggatc
     3601 gggccaggac ctgtcgatca ccaacctgac cgggcacccg tgcgccgtct ttccaatggg
     3661 cttccgcgac cgcgacggac gcttgatgcc cggttcggtt acgctgaccg gccggctcta
     3721 tgacgagaca accctcctgg ctgtcgcgca cgccttccaa caggcgaccg gcgaccacta
     3781 caaacggccg ccagtcgagg cctacctcgc cgacgaacag aaacggattg aacaggaaaa
     3841 atcgaaggcg aaagaagcgg cgggctgagg tgcgcgacgg cacgttgtgt cgcctcaggt
     3901 tcaccgagag cttcgatcgc tttttacgcc aacgtgtagt cgatgtacaa ttgcaggacc
     3961 taggcgtcgt agtcgcaagt gcggagactc cccatgaagt acaaaatcgt cctcgaagaa
     4021 tccgaagaag gcttcgcggt ctccgcgccg ggtctgccag gttgtcactc tcagggtgcg
     4081 acggaagagg aagccctgga gaacatcaag gacgctattc gcgaatacct gatgtcgatc
     4141 aacgatcgtc tccgcactgc gaacgtgcgg gaggtcgagg tcgagatcta accatgccca
     4201 ggctgccggg cgtgaatcat cagagcgcgg ttcgcgcatt agaaaaagcg ggattctggg
     4261 tcgatcgtca gggtaaacac atcgtgatga ccgacggtac acggacgatc acaatcccca
     4321 ggcacaatcc aatccatgcg at
//
LOCUS       DATAQL010000974         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1623824, whole genome shotgun sequence.
ACCESSION   DATAQL010000974 DATAQL010000000
VERSION     DATAQL010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623824"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /gene="ftsH"
                     /locus_tag="VHS97_16865"
     CDS             <1..1363
                     /gene="ftsH"
                     /locus_tag="VHS97_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX3449929.1"
                     /translation="EIVEFLKNPEKFQRLGGRIPKGVLLNGPPGTGKTLLARAVAGEA
                     GVPFFSISGSEFIQMFVGVGASRVRDMFKTAKENSPCILFIDEIDAVGRVRGAGLGGG
                     HDEREQTLNQILTEMDGFSPSESVIVLAATNRPDVLDPALLRPGRFDRHVTVDRPTKK
                     GRLEILKVHTRNTPLAADVDLESIARGTVGMSGADLANLVNEAALLATREDKAALDNA
                     DFDAARDKIIMGAKREEVITVKEKRSTAYHEVGHTLVAWYIPLADPVHKVTIIPRGRA
                     LGVTQTLPTEDRISYYGRDIKAMLAVYLGGRAAERLAFDDLSAGVADDLKRATRLARV
                     MVTQCGMSERVGPVFFRDSEEHPFLGREMSETRDHSEHTAQLIDEEVGRILREADDRA
                     YRILEEHREDLERLTEALIEREVLSVSEIEEIIGKRPTDDPETVGARLRGDEVVASID
                     NPS"
     gene            1479..>1940
                     /locus_tag="VHS97_16870"
     CDS             1479..>1940
                     /locus_tag="VHS97_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX3449930.1"
                     /translation="MSSAPPLTPSRFPVVCAGLVVADHLCPPLDHLPRAGELVTIDEL
                     VLNIGGGAANTAVDLARLGITATIRARVGNDIFGRFATETLKAHNVDTSALKIDPELA
                     TSQTLIVNVRDQDRRFIHCVGANTGFVAADLDAVLEYSPRVLHIGYFLILPR"
BASE COUNT          379 a          612 c          602 g          347 t
ORIGIN      
        1 ggaaatcgtc gagtttctca agaaccccga aaagtttcag cggctggggg gccggattcc
       61 caagggtgtt ctcttgaacg gaccgccggg aaccggcaag acgctgctgg cccgcgccgt
      121 cgccggcgaa gcgggtgtgc cgtttttctc gatctcgggc tccgaattca tccagatgtt
      181 cgtcggagtc ggtgccagcc gcgtccgcga catgttcaag accgccaagg agaacagccc
      241 ctgcattctc ttcatcgacg agattgacgc tgtcggtcgc gtaaggggcg ccggtctggg
      301 gggcgggcac gacgagcgcg agcaaacgct caatcagata ttgaccgaaa tggatggctt
      361 ttccccgagc gagagcgtga tcgtgctggc ggcgaccaat cgacccgacg tcctcgatcc
      421 ggcattgctt cgaccaggcc ggttcgatcg ccatgtcacg gttgatcggc ctaccaaaaa
      481 ggggcggttg gagatcctca aagtccatac tcgcaacact ccgcttgccg cggatgtgga
      541 tctcgagtcg atcgcccgcg gcaccgtcgg catgagtggt gccgacctgg cgaacctggt
      601 gaacgaggcc gctctgctgg cgacgcgcga agacaaagcc gcgctcgata atgccgactt
      661 tgatgcggcc cgcgacaaga tcatcatggg ggccaagcgc gaagaggtca tcaccgtaaa
      721 ggaaaagcga tcgaccgcat accacgaggt cgggcatacc ttggtcgcgt ggtacatccc
      781 cttggccgat ccggtccaca aggtcacgat cattccccgc ggccgggcgc tgggtgtgac
      841 ccagactttg cccaccgaag accgcatcag ctattacggg cgcgacatca aggcaatgct
      901 cgcggtctac ttgggtggcc gggccgccga acgccttgcc tttgacgacc tatccgccgg
      961 cgtcgccgac gacctaaagc gggcgaccag gcttgcccgg gtcatggtta cgcagtgcgg
     1021 catgagcgag cgcgtcggcc cggtcttttt tcgcgattcg gaggagcatc cgttcttagg
     1081 acgcgagatg agcgagacgc gcgaccactc cgagcacacc gcacagctca ttgacgaaga
     1141 ggtcggccgc atcctccgcg aggctgacga tcgcgcctac cggattctcg aagaacatcg
     1201 cgaggacctc gaacgcttga ctgaggccct gatcgaacgc gaggtgctgt ccgtgtcgga
     1261 gatcgaggaa atcatcggca agcggccgac cgatgatcca gaaacggtgg gcgcacggct
     1321 tcggggcgac gaggtcgtcg catcgatcga caacccttca tagatcgacg acccttcata
     1381 attgacgagc gagtgaacag tcagaacgca agatccaagc ccttggcggg acatggggcc
     1441 cgacggggcg acccgttgac cgcattggaa tatcgaacgt gtcgagcgct cccccgctga
     1501 ctccgtccag gttccctgtc gtgtgcgctg gactcgtggt ggccgatcat ctttgtccgc
     1561 cactcgacca tctgccgcga gctggcgagc tcgtgactat cgacgagctg gtgttgaaca
     1621 tcggcggcgg agcggccaac acggccgtcg atctcgcccg gctggggatc accgccacaa
     1681 tccgtgcccg ggtcggcaac gacatcttcg gccggttcgc caccgaaaca ctcaaagctc
     1741 acaacgtcga cacgagtgcg ctcaagatcg acccagagct cgcgaccagc cagaccctga
     1801 tcgtcaacgt tcgggaccag gaccggcggt tcattcactg cgtcggcgcg aacacgggct
     1861 ttgtcgccgc cgacctggac gcagttctgg aatactctcc tcgcgtgctg cacatcggct
     1921 acttcttgat cctgccgcgt
//
LOCUS       DATAQL010000975         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3612136, whole genome shotgun sequence.
ACCESSION   DATAQL010000975 DATAQL010000000
VERSION     DATAQL010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3612136"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHS97_16875"
     CDS             <1..691
                     /locus_tag="VHS97_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3449931.1"
                     /translation="ELKTMVEDVGLQTIGLHWLLAKTEGLYLTTPDAAVRRQTGDYLV
                     ALADLARDLGGSLLVLGSPKQRDLLPGVTNDQALGYATEVFERVMPAIAGAGIDLCLE
                     PLAPNDTDFINTCAQAMDVIGKVAHPHFKLHMDVKAQSSEPGATVVELIGRYAGLAGH
                     FHAQDVNLRGPGMGDVDFGPIIRALVEARYDRWVSVEVFDFSPGAEETARRSIQCLSA
                     SLESALAWSSS"
     gene            1036..2412
                     /gene="clpX"
                     /locus_tag="VHS97_16880"
     CDS             1036..2412
                     /gene="clpX"
                     /locus_tag="VHS97_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX3449932.1"
                     /translation="MPSGKDITGGGSSGGGKRNGGGNVSSGTTGGPKKNAYCSFCRKS
                     YRDVGPLVEGPGDVYICGDCIELCQQILDQERKRRGVPKTLFTDIPTPREIKDQLDAY
                     VIGQERAKKVLSVAVHNHYKRLVHGEDPETEVELDKSNILLIGPTGCGKTLLARTLAR
                     VLNVPFAIGDATTLTEAGYVGEDVENILLKLLHAADFDLESAQRGIIYIDEIDKIGKT
                     THNVSITRDVSGEGVQQALLKMLEGTVANVPPQGGRKHPEQQYIQMDTTNILFICGGT
                     FVGLENIIARRVGRKTIGFGSQTQAEQHTDLGELLGKVTSDDLLEFGMIPEFIGRLPV
                     ISPLMPLDVNALVQIMTEPRNALVKQYRRFFDMEGADVEFTADALQEIAKMAKAKDTG
                     ARGLRSIVEDIMLDIMFELPDRAAKDKGKFMVTGEVVRKEKSLFDTAPVPLTTSKASE
                     RERKKESA"
BASE COUNT          567 a          770 c          838 g          521 t
ORIGIN      
        1 cgagctcaag acgatggttg aagatgtggg gctgcaaaca atcggcctgc actggctact
       61 ggccaaaacc gaagggcttt acttgaccac gcccgacgcc gccgtgcgcc gtcagacagg
      121 cgactacctg gttgcactgg ccgatctcgc tcgcgatctc ggaggctcgt tgctcgtgct
      181 cggttccccc aagcagcgag atctcttgcc cggtgtaacc aatgaccagg ctctcggata
      241 cgcgaccgaa gtctttgagc gggtcatgcc ggcgatcgcg ggggcgggca tcgacctttg
      301 cctcgagccc ctggcgccca acgataccga ctttatcaat acctgtgccc aggcgatgga
      361 cgtgatcggg aaggtcgctc acccgcattt caagttgcac atggatgtga aggcccaaag
      421 cagcgaacca ggcgcgactg tcgtcgagct gatcggtcga tatgctggtc tcgccggcca
      481 tttccatgcc caggatgtca acctgcgggg gcccggcatg ggggacgtcg acttcggccc
      541 gatcataaga gcgctggtcg aagcgcgtta cgatcggtgg gtgtctgtcg aggtgttcga
      601 cttttctcca ggagcggaag agacggcgcg gcggagcatc cagtgtttga gtgcgtcttt
      661 ggagtcggcc ctggcctggt cttccagctg agtcgccgag tcgaccggcc ttgcgatgag
      721 ggtgtaggtg ctctaagata catgaagatt tgaggagagg gcagacgagg agatttcatg
      781 cgcgacggta ctcggtggct gccgggtgct ccggatatgt tgccatgacg gcaaattgcg
      841 tttcttcggg aaatcggttg atcgcggccg gtcaggatct ttttcctccc gctcgtgaat
      901 gagctatgat gttgcagtcg atcggggatg agcgtcgggc cgctttgcgt gggttcgagc
      961 agcgatagcg cgaaatcacc gcctcgcgcc ggcggtgttt catatagtcc ctacaagggg
     1021 acgaaaggat gaggtatgcc atcgggtaag gacatcacgg gcggcggctc gtcgggaggg
     1081 ggaaaacgaa atggcggtgg caacgtgagc tccggaacca cgggtggccc caagaaaaac
     1141 gcctattgct ccttctgccg caagagctat cgcgacgttg gccccctggt ggaaggtcct
     1201 ggagacgtat acatttgcgg cgattgcatc gagctctgcc agcaaatcct cgatcaagag
     1261 cggaaacggc gtggcgtccc caagactctc tttaccgaca tcccgacgcc tcgagaaatc
     1321 aaagatcagc tcgacgccta cgtgattggc caggaacggg cgaaaaaagt tttgtcggtg
     1381 gctgtgcaca accactacaa gcgactggtc catggcgaag atcccgagac tgaagtcgag
     1441 ctggataagt ccaacatcct cttgatcggg ccgaccggct gcggcaaaac cctgctggca
     1501 cgtacgctgg ctcgggtctt gaacgttcct tttgccatcg gtgatgcgac cacgctcacc
     1561 gaggccgggt acgtggggga ggacgtcgaa aacattctgc tcaagctgct gcatgccgcc
     1621 gatttcgatc tcgaatccgc ccagcgtggc attatctaca tcgacgaaat cgacaagatc
     1681 ggtaagacca cacacaacgt gtcgatcacg cgggacgtgt ccggcgaggg ggttcagcag
     1741 gccctcttga agatgctcga agggaccgtg gccaacgtcc cgcctcaggg tggccgcaag
     1801 catcccgagc agcagtatat ccagatggac acgacgaaca tcctgttcat ctgcggcggc
     1861 acgttcgtcg gactcgaaaa catcatcgcg cggcgggtcg gccgcaagac gatcggtttc
     1921 ggcagccaaa cccaggccga gcaacatacg gatctgggcg aacttctcgg caaggtcacc
     1981 tccgacgact tgctcgagtt cggcatgatt cccgaattca tcggccgact tccggtcatc
     2041 agcccgctca tgcctctgga tgtcaatgcc ctcgtccaga tcatgactga gccacgcaac
     2101 gcactcgtca agcagtatcg tcgattcttc gacatggaag gtgccgacgt cgaatttact
     2161 gccgacgcgc tgcaagagat cgccaagatg gccaaggcca aggacaccgg tgcccgcggt
     2221 ttgcgatcga tcgtcgaaga catcatgctc gacatcatgt tcgagttgcc cgatcgggct
     2281 gccaaggaca agggaaagtt catggtcacc ggcgaagtgg ttcgcaagga aaagagcctg
     2341 ttcgacacgg ctccggtgcc cttgaccacg tccaaggcgt ccgagcgcga gcgcaagaaa
     2401 gagagcgctt aacgcacgtc gcggaacgcc tgcctctgac gagtgcgctt gtgatcgacg
     2461 ttcaactcgg gtgaacgcca tgagcacagc cgagcgcatc aagccggaga ttcttctgcc
     2521 gctcatcgcc ggtcagtgcc aatagcaggt cagcgcgggc gggaccgcgc caacctgcga
     2581 agtaaatcga gccccaggcg cagggtcgcg tcgtcgacgg cgtacgagat gcgaaagtgc
     2641 gtgtcgtgcg tagagaacac gtttccgggg atgatcaaga gattattctt gatcgc
//
LOCUS       DATAQL010000976         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2391845, whole genome shotgun sequence.
ACCESSION   DATAQL010000976 DATAQL010000000
VERSION     DATAQL010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2391845"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1922
                     /locus_tag="VHS97_16885"
     CDS             246..1922
                     /locus_tag="VHS97_16885"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3449933.1"
                     /translation="MHRNRKFFGVSRIALSTAWLALLGPSVAALAAPPNAGTATPIEH
                     LVVIFQENVSFDHYFATYPVASNPASEPQFLAKEGTPSVNGLGTLDDEGNPVGALLID
                     NPNARNPGNDGNAINPFRLDRSQASTCDQDHAYGDEQKAFDQGLMDMFPGSVGAGGSS
                     FCNAAFAYGKGKGLVMGYFDGNTVTALWNYAQNFAMSDNSYGTTFGPSTPGLLNLVAG
                     NTFPATPSAPTSKVVPLNSGPGTLVGDLDPTGDVCSTGTPTVRLGGPNIGDLLNQKGV
                     TWGAFMGGFDLTVQNSNHTTGCARSSPATPANGGPTTDYIPHHAFFQYYASTANPQHT
                     RPSSVGAIGTSHDGGANHQYDLHDFFDALAAGNMPAVSIIKAIAAGDGHAGYSDPLME
                     QSLTLVPTINAIMGSRFWKSTAIVILYDDSDGWYDHQMSPIVNPSAVLQAASASNSDQ
                     LNAPGVCGNGTPIGGIEGRCGYGPRQPLLVISPFAKKNFVDHTLTDQSSVIRFIEENW
                     GTGQIGGDSYDQIAGTRANMFDFNGGNAFGERRLLLDPMTGEPSRGHQDK"
BASE COUNT          419 a          661 c          655 g          413 t
ORIGIN      
        1 atttccgtat cgattgacgt ttagccgtgc cagcgatcat gaggcggcga cattcgtcga
       61 aggtattgac tggaaccgac aagcccgatc aatccggatc tacaactcgg ttgatctcgg
      121 cgccgggcgg acggtcggaa cgtaacggtt gagccaccca agcatcatcg aactgacatc
      181 tggctgtaat gcgcggtcga ttgaatgcgt ccctagctca actgacccgc gaagggggaa
      241 ttgggatgca ccgcaatcgg aagttctttg gggtatcgcg catcgccttg agcactgctt
      301 ggcttgccct gctcgggccc tccgtggcgg cgctggcggc cccgcccaat gcgggcaccg
      361 cgacgccgat cgagcacctg gtcgtcattt ttcaggagaa cgtgtcgttc gatcattatt
      421 tcgcgaccta tccggtcgcg tcgaacccgg cgagtgaacc ccagtttttg gcgaaagagg
      481 ggacacctag cgtcaatggc ttggggacgt tggacgacga aggaaacccg gttggtgctc
      541 tgctgatcga caatccgaac gcgcgaaacc cagggaacga tggtaacgcg atcaatccgt
      601 tccggctcga ccgctcgcag gcctcaacct gcgatcaaga ccacgcttac ggggacgagc
      661 agaaggcgtt cgaccagggt ctgatggaca tgttcccagg cagcgtcggg gccggtggta
      721 gttcgttctg taacgccgcc ttcgcctacg gaaagggcaa gggcctagtg atgggctatt
      781 tcgacgggaa caccgtcacc gccctgtgga attacgcgca gaatttcgcg atgagcgaca
      841 attcttacgg cactaccttt ggtccatcga cgccgggtct tctcaacctg gtcgcgggga
      901 acactttccc ggcgacgccg agtgccccga cgagcaaggt cgtgcccctg aactcgggac
      961 cgggcaccct ggttggcgac ctcgacccga cgggcgatgt ttgctcaacc ggcaccccca
     1021 cggtgcgcct tggcggaccg aacatcggtg atctgctgaa ccagaagggt gtgacttggg
     1081 gcgccttcat gggtgggttc gacctgactg tccaaaactc caaccacact accggttgcg
     1141 ctcgctcgag cccggcgact ccggccaatg gcggcccaac taccgattac atcccgcacc
     1201 acgcgttttt ccaatactac gcttcgacag caaatccgca gcatacccgg ccaagctcgg
     1261 tcggggccat cggcacctcg catgatggcg gggccaacca tcaatacgat ctgcacgatt
     1321 tttttgatgc gctcgcggca ggaaatatgc ccgctgtcag catcatcaag gcgatcgccg
     1381 cgggggacgg gcatgcgggc tattccgacc cgctgatgga gcagtcgctt acgctggttc
     1441 cgacgatcaa tgcgattatg gggtcgcgtt tctggaaatc gacggcgatc gtcatcctct
     1501 atgacgactc cgacggctgg tacgaccacc agatgagccc gatcgtcaat ccgtctgcag
     1561 tcctacaagc cgctagcgcc agcaacagcg accagctgaa tgctcctggt gtctgtggca
     1621 atggcacccc gatcggcggg atcgagggac gctgcggcta cggtccgcgg cagccgctgt
     1681 tggtgatctc gccctttgcg aagaagaact tcgtcgatca cacgctgacc gatcagtcct
     1741 cagtgatccg ctttatcgag gagaactggg gcaccgggca gatcggcggc gactcctacg
     1801 accaaatcgc cggcacgcgg gcaaacatgt tcgacttcaa tggcgggaac gctttcggcg
     1861 agcgccggct gctgctggat cccatgaccg gcgagccttc acgcggccat caagacaaat
     1921 aggcgcgatt ttgcgtagtt cgggccgtga ggccaaagct ggaggcggcg gcgttgagag
     1981 ccgtcgcctc cagctcggtc ggctcgcgcg tcccgtcggc gcataaagac agaccctcag
     2041 cgaggagacc tgagcgcccg tccttacggt attcccctcc ctatctcggc cggggccttt
     2101 acctcgttgg cgcgagttgt ggatgaagat cgcgccaacc gagggagg
//
LOCUS       DATAQL010000977         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2696682, whole genome shotgun sequence.
ACCESSION   DATAQL010000977 DATAQL010000000
VERSION     DATAQL010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696682"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3292
                     /locus_tag="VHS97_16890"
     CDS             <1..3292
                     /locus_tag="VHS97_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449934.1"
                     /translation="QMQMQMQMQMQMNMGMQFGMSSPGAPPPQQFMFEKNVFTNQDIF
                     GLAGAAPHGLDDAGLKGLGQILRQALDSGNVVEDFVARLRTVLKAPAQKAPLSERQAV
                     KILFAADCPVEVGEFLPNPEKAEADNDREALNLLARHYLAIYDRDKKKIDLERAWKVT
                     QAALATGKVERAQKEEAIRRAVELTPKIRAELGRTWLEESFTQRPERGMEIIAAIGSA
                     SSGGLQNHAFDGDFRLKSLELQKLAVEALLQKAPRLGHDWASSLALLAEAWLHEAEFS
                     YHYDYSTSLGPRMQFDRYGNVYYSSYDPFTPDMMARQRGMPLAIKVGDVVNNMPGDNW
                     LEYVDPGMKPKFATVFAQLYLKVSEDEKAYPYIEKLAQSNPRKAKDLAEEFVRVWTKN
                     HNPNSQQLRRSRFFYIYGFDNRSEGIPLTRSKQERNLVELAHLVKRLRKLPIAEVDEK
                     LLTEAFTTCHSTAEVYRLDALEKVFGSLDGIKPATLAELIQQMRGNLLGVWRQPAEQE
                     KNKTKRREKDIRAEVLRGYEVARAVVDRGLARHPDQWALVLAKATIMHDENNYLQEIE
                     RSTEFAPRRQAAFAEFQRAAQLYVKAVPSIEQSDETVQVFDYWYSASLGACDPQNITE
                     ETLADLRQPKRIRETLESIKGEPGDRHQNKFANSLFTRLNMVKPSIKFRYLKAGFEIV
                     GDNPQAFDARKVFDYYKDLVTEIKLETKVDGSDAIGHGQPFGLFVNLRHTREIERESG
                     GFSRYLQNQNNAGFSYYNFGRPLENYRDKFQDAAKQALQEHFEVMSVTFQDEKVNSRA
                     LGEYGWRVTPYAYILLKARGPKVDKVPPLRLDLDFMDTSGYVVLPVESPALPVDATPT
                     KGSVRPFEKLQITQTLDERQAADGKLILEVKGTARGLVPDLENIVELDHPGFQVDKVD
                     DQGISVSRFDPDSDATLIDSERTWLVAFRAAEGQEKPPATFRFAKATVDGAEVAYQRY
                     VDADLAKVGPEISLEARYDRPRYAWLWWAGASLLGLLIAAILVWKLSARPKRPVALRF
                     EMPNQVTPFSVLGLLRQIERTNGLAPPQMQELAGSIDRIERQFFAESDGESIDLRHVA
                     ETWIRRAT"
     gene            3301..4521
                     /locus_tag="VHS97_16895"
     CDS             3301..4521
                     /locus_tag="VHS97_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449935.1"
                     /translation="MNRTGVLSALAVLLASSPSFAQFGGMGGMGGGGGPGAQGMMMQG
                     GGFAGHGFAEVRRSVQIEMEGGQRLSGRMDLRPIIVDGDLGRYTIAPDKIKMIRFLKP
                     ANEVEGDADGEGGGDGKVVVPVGLQNQVVAMRPVRGGRGGFGGMADRDPQTGATLTRG
                     KVITTTANEIIGTIHIPMDFRLDLDFATLNLSPTKLRSITFTGETPHDKPAPAEAAAL
                     RTPEGVGPVAQSEAASPPRYFRQGSSLVVISPVGDRVALLDLKTKKSQTLELSGSKDA
                     PLEVTPIPTENIVALRLRGSKITRIAVADTTRSIWHSQELRTPIEGQALPIVAAGVVY
                     YILGRDVYAYGTEAQRWDVAALAEGVRVMPFVEPGAVTIEGQGHIYMFAGKSGRWEHV
                     DVRTVLDVGEPEKK"
BASE COUNT         1027 a         1425 c         1453 g          898 t
ORIGIN      
        1 gcaaatgcag atgcaaatgc aaatgcagat gcaaatgaac atgggaatgc aattcggcat
       61 gagctcgccg ggggcgcccc cgccccagca gtttatgttc gagaaaaacg tcttcacaaa
      121 tcaggatatt ttcggtctgg cgggcgcagc ccctcacggt ctggatgatg ccggactcaa
      181 ggggctggga cagatcctcc gccaggcgct cgattcaggc aatgtggtcg aagacttcgt
      241 tgcacggctc cggacggttt tgaaggcacc ggcccagaag gcgccactat cggagcgcca
      301 ggcggtcaag atcctgttcg cggccgattg ccccgtcgaa gtgggcgaat ttttgccgaa
      361 tcccgagaaa gccgaggccg acaacgatcg cgaagccttg aacctgctcg cccgccatta
      421 cctggcgatc tacgatcggg acaagaagaa gatcgatctc gagcgcgcct ggaaagtcac
      481 gcaggctgcg ctcgcaaccg gcaaggttga gcgagctcag aaagaggaag ccatccgccg
      541 cgccgtcgag ctcacgccca agatccgcgc agaactcggc cgcacgtggc tcgaggagag
      601 ttttacccag cggcccgagc gtggtatgga gattattgct gccatcggct cggcctcatc
      661 gggggggctg caaaatcatg cgtttgacgg agatttccgg ctcaagtcgc tcgagcttca
      721 aaagttggcc gtcgaagcct tgttgcagaa ggcgccccgg ctcggccacg attgggcgag
      781 cagcctggca ctactggccg aagcctggct gcacgaggcc gaattctcct atcactacga
      841 ctactcaacc agcctcgggc cccgcatgca gttcgaccgg tatggcaacg tctattattc
      901 cagctacgac ccattcacac ccgatatgat ggcgcgccag cgcggcatgc cgctggcgat
      961 caaggtcggt gacgtcgtca acaacatgcc gggagacaat tggctggaat atgtcgatcc
     1021 ggggatgaag cccaagtttg ccacggtttt cgctcagctc tatctgaaag tgagcgagga
     1081 cgagaaggcg tacccgtata ttgaaaagct agcccaatcc aatcctcgca aggccaagga
     1141 tctggctgaa gagttcgtcc gcgtctggac caaaaaccac aatcctaact cgcagcaatt
     1201 gcggcgcagc cggttcttct acatttacgg cttcgataac cgatcggagg ggatcccgct
     1261 gacgcgctcg aagcaagagc gcaacctggt ggagctggcc catctcgtca aacggttgcg
     1321 caagctcccc atagcggagg tcgatgaaaa gcttttgacc gaggcgttca cgacctgcca
     1381 tagcacggcc gaggtttatc ggcttgacgc tctcgaaaaa gtcttcggct cactcgacgg
     1441 aatcaagccg gcgactctcg ccgagctgat ccagcagatg cggggaaatc tgcttggcgt
     1501 ctggcgccag cccgccgagc aggaaaagaa caagacaaag cgccgcgaga aagacatccg
     1561 cgccgaggta ctccgcggtt acgaagtggc gcgtgcagtc gtcgatcgcg gccttgccag
     1621 gcatcccgac cagtgggcgc tcgtcctggc caaggcgacg atcatgcacg acgaaaacaa
     1681 ctacttgcaa gagatcgagc ggtcgaccga gtttgcaccc aggcgtcaag cggcgttcgc
     1741 cgagttccag cgggcggctc agctttatgt caaagccgtc ccctcgatcg agcaaagcga
     1801 tgagaccgtc caggtctttg attactggta ttccgccagt ctgggcgcct gcgaccccca
     1861 aaatatcacc gaggaaacac tcgccgacct gcgacagcca aaacggatcc gcgaaacgct
     1921 cgagagcatc aaaggcgagc ccggcgatcg gcaccagaac aagtttgcca actcgctttt
     1981 tacccgattg aacatggtca aaccgtcgat caagttccgc tacttgaaag cggggttcga
     2041 gatcgtcggc gataatcccc aggccttcga tgcacgcaag gtattcgact actacaaaga
     2101 cctggtgacc gagatcaagc tcgagaccaa ggtcgatggc agcgacgcga tcggccacgg
     2161 tcagccgttt ggcctctttg tcaatctgcg ccacacccgc gagatcgaac gcgagtcggg
     2221 tggcttttcg cgctatctcc agaatcagaa caatgcggga ttttcctatt acaattttgg
     2281 ccggccgctc gagaactatc gggacaagtt tcaggacgcc gccaagcagg cgcttcaaga
     2341 acattttgag gtcatgtcgg tcacgttcca ggatgagaag gtgaattcga gggcactggg
     2401 cgagtatggg tggcgagtca caccgtatgc ctacattctg ttgaaggccc gcggccccaa
     2461 agtcgacaag gttccgccgc tgcggctgga tctcgacttc atggacacgt caggctatgt
     2521 ggtcctgccc gtcgaatcgc ctgcgctccc cgtggacgcg actccaacca agggctcggt
     2581 gcggccgttt gaaaagctac agattactca gaccctcgac gagcgccagg ctgccgatgg
     2641 caagctgatt ctcgaagtca agggaaccgc ccgaggtctc gtgcccgacc tggaaaacat
     2701 cgtcgagctc gatcaccctg gatttcaggt cgacaaggtc gatgaccagg gcatttcggt
     2761 ctcgcggttt gatcccgata gcgacgcgac cctgattgat tcggagcgca cctggctggt
     2821 cgcttttcgc gcagcggaag gtcaggagaa accccccgca acgtttcgat tcgccaaagc
     2881 gactgtcgat ggagcggagg tcgcttatca gcgctacgtc gatgccgacc tggcgaaggt
     2941 cgggccggag atctcgctcg aggcgcgtta cgaccggccg cgatatgcat ggctctggtg
     3001 ggccggggcc agtctcctgg ggctcttaat tgcggccatt ctcgtctgga agctatccgc
     3061 tcgccccaaa cggcctgttg cgcttcggtt tgaaatgccc aaccaggtca ccccattttc
     3121 ggtgctcggc ctcttacgac agatcgagcg caccaacggc ctcgcaccgc cgcagatgca
     3181 agagctggcc ggctcgatcg accgcatcga gcgtcagttt tttgccgagt cagacggcga
     3241 atcgatcgac ctgcgccacg tcgccgagac ctggattcgg agggcgactt gaagtcatta
     3301 atgaatcgaa caggagtgct ctcggcactc gccgtgttgc tcgcatcgtc gccaagcttc
     3361 gcccagttcg ggggcatggg tggcatggga ggtgggggcg gcccaggcgc gcaggggatg
     3421 atgatgcagg gcggtggttt tgccggccat ggcttcgcag aagttcggag atccgtccag
     3481 atagaaatgg agggcggcca acgcttgagc ggccggatgg atctccggcc catcattgtt
     3541 gacggcgatc tcgggcggta taccatcgca cctgacaaga tcaagatgat ccggttcttg
     3601 aagccggcaa acgaggtcga gggggacgcc gatggggaag gcggcggcga cggcaaggtg
     3661 gtcgtccccg tggggctcca gaatcaagtt gtggcgatgc gcccggtacg cggggggaga
     3721 ggaggctttg ggggaatggc cgacagggat ccccagacgg gcgcgacgtt aacccgcggg
     3781 aaggtgatca caacgacggc taacgagatc atcgggacca tccacattcc gatggacttc
     3841 aggctcgatc tcgacttcgc cacgctgaat ttgtcaccga ccaagctgag atcgatcacg
     3901 ttcaccggcg agactccgca cgacaaacca gccccggccg aagcggcagc ccttcgcacg
     3961 cctgagggcg tcggcccggt cgcccagagt gaagccgcaa gccctccccg ctacttccgg
     4021 caaggtagtt ccctcgtcgt catctcgccc gtaggggacc gggttgcgct cctcgacctc
     4081 aagacgaaga agtcccaaac cctcgagtta tctggatcga aagacgctcc gctcgaagtt
     4141 acccccattc ccaccgagaa catcgtggca ctgaggttga gaggatcgaa gattacccgg
     4201 attgccgtcg ccgacactac cagaagcatc tggcactccc aagagctgcg cacaccgatc
     4261 gagggtcagg ctctgccgat cgtggcggcc ggagtggtct attacattct gggccgagac
     4321 gtttatgcgt acggcacgga ggcgcagcgc tgggacgtcg ccgcgttggc cgaaggggtc
     4381 cgggtcatgc ccttcgttga gcccggagcc gtcacgatcg agggccaggg ccacatctat
     4441 atgttcgcag gcaagtcagg caggtgggag catgtcgacg tgcgcaccgt ccttgatgtc
     4501 ggcgagccgg agaagaagta gttggtggga cggagagcct cgagattggc tttctcgtag
     4561 gcttccgaaa ggtcatagat ctcgacctca ctgcgccttg acatcggagt cgtcccttgc
     4621 cgcgtcggct tgccagcgga tcgaatctca ctgtaggttt ggcgaaacgg taaaatgaaa
     4681 ccaacctcaa gccccgggaa tcaaagacac ttgtccgccc aatactccag ttcggataac
     4741 tcatctctcg gcaccatcga cacgtccgtt tcgcgactga tctttctggc gatgctgggt
     4801 ctc
//
LOCUS       DATAQL010000978         5012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3764445, whole genome shotgun sequence.
ACCESSION   DATAQL010000978 DATAQL010000000
VERSION     DATAQL010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3764445"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VHS97_16900"
     CDS             <1..714
                     /locus_tag="VHS97_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX3449936.1"
                     /translation="EVKVGPGRIMCGSLFLALALFMAPALFGRPPASLVWDRLIVGIL
                     PPDSNELSADVQIAGKPDESAGSGSLKEVKATSTDPAKAEREEKKAHGVLWGMSFDQA
                     RELAATEKKPVLIDFTGVNCANCRLMERRVLPRPEVVALLKKFVTVQLYTDFVPINSI
                     TADQRQERAELNQERILKLVQEATNPYYVVLSPDGEVLGRIGGYNEPPVFVDFLTKAL
                     GKLPEAVKVTQSESANEAR"
     gene            1086..1463
                     /locus_tag="VHS97_16905"
     CDS             1086..1463
                     /locus_tag="VHS97_16905"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3449937.1"
                     /translation="MTTERRTIADLSPEVQIRVAAARANRRTPEGRAEIERIRAEIRE
                     DYPPLEPDPELAKVLDALRVERERQGLSLADLAERTQMDAPTLSDLESGKLANPTVQA
                     LRAYAHALGKQLAWKIEDLVRTT"
     gene            complement(1560..2579)
                     /locus_tag="VHS97_16910"
     CDS             complement(1560..2579)
                     /locus_tag="VHS97_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX3449938.1"
                     /translation="MNHRPIILSLTNMHANGLALLRAESELRMASALDPVTLQREIVG
                     ADGLIIRTGGVVDAALLKKGDKLKVVGRHGVGYDQIDVDAATALGIQVVYTPGANTQS
                     VAEHVFTLMIGLSKHFPRMMAGLAAGNYSLRTSMTGREIAGKSLGIIGFGRIGRRVAE
                     IAYLGFGMKVLYHDIVAAPDEIDQLSNARKVDLKVVLESCEYVTLHVPLDPSTRGMIN
                     RASLALMRNDAILINTSRGPVVDEIAVAEALDAHRLWGYGADVFTVEPPPAGHPLIGR
                     SDVMLTPHSAAQTEEGLRAMATTVAQEVLGVLRGHKPNYPVNNPLEVEGVRKSLGLEP
                     IYGAH"
     gene            2652..3911
                     /locus_tag="VHS97_16915"
     CDS             2652..3911
                     /locus_tag="VHS97_16915"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX3449939.1"
                     /translation="MTLAGHTQLMFHRSNSVALWAVVVLGVLLRGAGMLQGARAFDDP
                     DNYLPLARAVAAGDGFSLNGRPTAYRPPLYPLLLAPSISVLGERVAWGIALFHLALGA
                     GTVLLTAAAARFSGLSPARSILAAFVTACDPVLVWQSRSVMTETPAAFLLAATLAALG
                     LRGWRGPVLGGVGLGLAALCRPSMLAGAALTLVAALVVHPGDRRTRLIRGTLLGMTVV
                     LVLFPWLVRNLLVFGEPIWTTTHGGYTLALANNPVYYDDVLRGPPGRVWTGHDQWLWW
                     DSVNRQTAGMTEAQADRYLRQTVWQVIRERPADFARATVARLGHFWSLAPAAAVYSGG
                     VRWATMAWTVPLWLAVALGLPRRDLWRWPRIAAPLAAIGLALVHGLFWTDLRMRAPIV
                     PAISLIAAGAGLPLATRKPAGELDEQR"
     gene            complement(3917..>5012)
                     /locus_tag="VHS97_16920"
     CDS             complement(3917..>5012)
                     /locus_tag="VHS97_16920"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX3449940.1"
                     /translation="AGFESVEVIVSRGRRDALLSTQPQDESLDDLSAEFGRCIWSDNS
                     EWPTLVRAGLAAASGDHFVVLDVNRHYTPESLIRVLDPIRGNQCDMAVAVPGRGRFRL
                     ASHLRSWLGLGFISRLFLGTSDVFSGLFALRRNAWDSEFKHRPARGSILVLDTLLRRQ
                     ARSMDVPVSVGNRFRTGRIGLKDVRPLKHLLDRRFGNYSRLVQFCIVGASGMFVDLSL
                     YALLQHLLSYTWLADRKSVLFGDTWHLVIAAGMSIGCALVWNFMLNRRLTFNDAHKGA
                     LLRQFLTYVLSNALAILLSFSVRLYLPAHVAFFERHRLAAAVVGIVAATGISFSMSRW
                     LVFSRRTEPKLVAHFPLDHPPAVGQSTGHP"
BASE COUNT          972 a         1506 c         1574 g          960 t
ORIGIN      
        1 gaagtcaagg tagggcctgg gcgaatcatg tgtggatctc tctttctggc gctggcgctt
       61 ttcatggcgc ctgcgctgtt tggccggccg ccggcgagcc ttgtctggga tcgtttgatc
      121 gtcggcatcc taccacccga ctccaacgag ctgtccgccg acgttcagat tgcgggcaag
      181 cccgatgagt ccgccggctc cggctcgttg aaagaagtca aggcgacatc aactgatccc
      241 gccaaagccg aacgcgagga aaagaaagct cacggtgtgc tctggggcat gagctttgac
      301 caagcccgtg agcttgccgc cactgagaag aagccagtac ttattgactt cactggcgtc
      361 aactgcgcga attgccggct catggagcgg cgagtcttgc cccggccaga ggttgtggcc
      421 ctgctcaaga agtttgtcac ggtccagctc tataccgact ttgtcccgat caactcgatc
      481 accgcggacc aacgtcaaga gcgggctgag ctgaaccagg aacgtatcct caagctggta
      541 caggaagcga ccaaccccta ttacgtggtt ctctcccctg atggagaagt gctgggccgc
      601 atcggcggct ataacgaacc gcccgtattc gtcgatttcc tgacgaaagc tctgggaaaa
      661 ctgccagagg ccgtgaaagt cactcagtcc gaatccgcaa atgaggccag gtgaatccaa
      721 tcggcggcac ttggtatggc aattgcggct ctgccggttg cctcgaaagg tatcaggcgg
      781 tatgagaatc cgggaatgct acatgcgttg atcgactggg acgatgggga tgacccgagt
      841 ggaaatgtcc agcacatcgc cgacaatggt ttgacgccca ccgaggtcga ggaagtcctg
      901 agagatccaa acagcaacga cgcagtgagc cggtcgagtg ggcgtcctat taggttcgga
      961 tggacgagta ctggaaaaca tattctggtg gcgtacgaga ttgaatgtga cgaccctctg
     1021 gttatctacc cggtgacagc atacgaggtg gccgagccag ggggtcacgt ggggaaccaa
     1081 acgaaatgac aaccgagcga aggaccatcg ccgacctttc tcccgaggta caaatccggg
     1141 tcgccgccgc gcgcgcaaac cgtcgcactc ccgaaggtcg cgccgaaata gaacgcatta
     1201 gggccgagat acgggaggat taccctcctc ttgaaccaga cccagaactg gcgaaagtgc
     1261 tcgacgccct ccgagttgaa cgcgaacgtc aggggctgag tctggctgat ctggctgaac
     1321 gcacccaaat ggacgcgccc actctctcag atctggaatc cgggaaactt gcgaacccga
     1381 ccgtccaggc ccttcgtgcc tacgcgcatg ccttgggcaa gcagcttgcg tggaaaatcg
     1441 aggacctcgt acgaaccacg tgacgacgtg agcgccagcg cgagcaggta atccatgata
     1501 cgcgcggagc accattacgt cgccgttatg ggccttccgg ttctctcaga tgcccgcaat
     1561 cagtgggcgc cgtagattgg ctccagcccc agactcttcc gaacgccctc gacctcgagg
     1621 gggttgttca cagggtagtt gggcttatgc cctcgcagaa cgcccagcac ctcctgggcg
     1681 acggtggtcg ccatggcgcg caggccttct tccgtctgag ccgcgctatg gggcgtgagc
     1741 atcacgtcgc ttcggccgat gagcggatgg ccggccggtg gcggctcgac cgtgaaaaca
     1801 tccgcaccgt agccccagag gcggtgggca tcgagggcct cggcgaccgc tatttcgtcc
     1861 accacgggcc cgcggcttgt gttgatcaag atcgcgtcgt tacgcatgag cgccaagctc
     1921 gcgcggttga tcatgccccg cgtgctcggg tctagcggaa cgtgcaaagt gacatactcg
     1981 caagattcga gaacgacttt caaatccact ttccgagcgt tcgaaagctg gtcgatctca
     2041 tcgggcgcgg ccacgatgtc gtggtagaga accttcatcc caaagccaag ataggcgatc
     2101 tcggcgacgc gacggccgat cctgccgaat ccgatgatcc ccaaactctt gccggcgatc
     2161 tcgcggccgg tcatgctggt tctgagggaa taattaccgg cggccaggcc ggccatcatg
     2221 cgcggaaagt gtttcgacag gccgatcatg agcgtgaaaa catgctccgc tacgctctgt
     2281 gtgttggcgc ctggagtgta gacgacttga atgccgaggg cagtcgccgc gtcgacgtcg
     2341 atttgatcgt agccgactcc atgccgcccg acgaccttga gcttgtctcc ctttttgagc
     2401 agtgcggcat cgaccacacc gccggtgcgg ataatgagac cgtcggcgcc cacgatctcg
     2461 cgctggagcg tcacggggtc gagtgcgctg gccattcgca gctcagactc cgcgcggagc
     2521 agcgctaggc cgttcgcgtg catgttcgtc aacgacagga tgataggacg atgattcatt
     2581 gagccgctca cggtttattc cttgccgcca gacgaaagaa atggattata cagccaggca
     2641 cctcggtacg catgaccctc gccggacaca cgcaactgat gtttcaccgt agcaattcgg
     2701 ttgctttgtg ggctgttgtc gtgctgggcg tgcttctccg tggagcgggg atgctgcaag
     2761 gcgccagggc atttgacgat cccgacaatt atcttccctt ggcgcgcgcg gtcgcggccg
     2821 gggatggttt cagcttgaat ggtcgtccga ccgcatatcg cccgcccctc tatccgctct
     2881 tactcgcgcc ttcgatttcg gttctgggtg aacgagttgc ctggggcatc gcgctttttc
     2941 accttgcatt gggggccggc acggtcttgc ttacggcggc ggcggccagg ttttcaggtc
     3001 tctcacccgc gcggtcgatt ctcgccgcgt ttgtgactgc ctgcgacccg gtcttggtgt
     3061 ggcagagccg ctcggtgatg acggaaaccc cggccgcgtt cttgctcgcc gcgacactgg
     3121 cggcgcttgg actacgagga tggcgtgggc cggtcttggg gggcgtcggg ttgggtctgg
     3181 cggccctgtg ccggccgagc atgctcgcag gagctgcgct tacactggtg gccgcgctcg
     3241 tggtccatcc aggcgatcgc cggacgcgtt taattcgtgg aaccctgctg ggaatgaccg
     3301 tcgtgctggt ccttttcccc tggttggttc gcaacctgct ggttttcggt gagccgatct
     3361 ggacgaccac ccacgggggt tataccctgg cgctggcgaa taaccccgtc tactacgacg
     3421 acgtattgcg tggtccgccg gggcgagtct ggaccgggca cgaccagtgg ctctggtggg
     3481 actcggttaa ccggcagacc gccggcatga ccgaggccca agccgatcgc tacctgaggc
     3541 aaacggtctg gcaggtcatc cgcgagcgac cggccgattt cgcgcgagcg acggtggctc
     3601 gtctcgggca tttctggagc ctggcaccgg ccgccgcggt ttactccggc ggcgttcggt
     3661 gggcgacgat ggcctggacc gtcccactct ggcttgcagt cgcgctcggc ttgccacgac
     3721 gcgacctctg gcgttggccg cgaatcgccg cgcctttggc cgcgatcgga cttgccctgg
     3781 ttcacggtct tttctggact gatttgcgga tgagagcccc gatcgtgccg gcgatttctc
     3841 tgatcgccgc cggagcgggt ctgccgctcg ccacgagaaa accggccggc gaactcgacg
     3901 aacagcgttg atcctctcag ggatggccgg ttgactgacc gactgccggc ggatgatcga
     3961 gcgggaaatg cgcgacaagc tttggctcag tccggcgcga aaagaccagc cagcgggaca
     4021 ttgaaaagct gatccccgtc gcggccacga tcccgacgac ggccgccgcc agcctgtgtc
     4081 gctcaaagaa tgcgacgtgg gcgggaagat aaagccgtac ggaaaagctc aggaggatcg
     4141 ccagtgcatt gctgaggacg taggtcaaga actggcggag tagtgccccc ttgtgtgcat
     4201 cgttgaatgt caaccgccgg ttgagcatga agttccagac caaggcgcac ccgatcgaca
     4261 tgccagcagc gatcaccaga tgccaggtgt caccgaaaag cactgacttg cggtcagcca
     4321 gccaggtgta cgatagcaag tgctgcagca gggcatagag cgagaggtcg acgaacatgc
     4381 ccgacgcccc gacgatgcag aattgcacga gtcgcgagta gttgccaaat ctgcggtcca
     4441 gcaggtgctt gagtgggcgc acgtctttca agccgatccg gcccgtccga aagcggttgc
     4501 cgacggaaac gggcacgtcc atggaacgtg cttgccgtcg caacaatgtg tcgagaacca
     4561 ggattgatcc ccgcgcgggt cgatgcttga actcagaatc ccacgcgttg cgacgcagcg
     4621 cgaacaggcc cgagaatacg tcggacgtgc ccagaaacag ccggctgatg aatcccagcc
     4681 cgagccacga tcgtaggtga ctcgccaatc gaaagcgccc gcgtccagga accgcgacgg
     4741 ccatatcgca ttgattgccg cggatcggat caaggactcg aatcagtgat tccggcgtgt
     4801 aatggcgatt cacatcgaga acgacgaagt ggtcgccgct ggctgccgcc aatcccgccc
     4861 ggacaagcgt cggccactcc gagttgtcgg accagatgca cctgccgaat tccgcagaaa
     4921 gatcgtccaa ggactcgtct tggggctgag tgctaaggag cgcgtcgcgc cggccgcggg
     4981 agacgatgac ttcgaccgac tcgaaacccg ct
//
LOCUS       DATAQL010000979         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4222097, whole genome shotgun sequence.
ACCESSION   DATAQL010000979 DATAQL010000000
VERSION     DATAQL010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4222097"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..863)
                     /locus_tag="VHS97_16925"
     CDS             complement(18..863)
                     /locus_tag="VHS97_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3449941.1"
                     /translation="MKACISQATTLKNPFEADLSLYGRYGWTAVELWLTKLESFLETH
                     SPAEALDLIDSSGLKPVAAASQGGLLLSQGAERIAHWDHFRRRLLLLQELNVPTLIIT
                     PDFVRSPGIAEYARAAQALGEAAQLAASFGVRIAIEFQKSSPLCACLETAIALIAQSG
                     ADNAAVCLDVFHYYTGPSKFEDLAHLSPQNLAWVQVCDVSGTPRELAGDADRILPGEG
                     DFELGPIFEHLERIGYDGYVSLEVLNPHLWQVAPDRVAELGYQALNRVLGHSHSTGLA
                     ARGGP"
     gene            complement(983..2494)
                     /locus_tag="VHS97_16930"
     CDS             complement(983..2494)
                     /locus_tag="VHS97_16930"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX3449942.1"
                     /translation="MDKLVSLCKRRGFIFPSSEVYGGINGFWDYGPLGVELKRNIKDA
                     WWRDNVRMRDDMVGLDCSIIMNPRVWEASGHVGGFSDPMVDCRATKERYRADQLYVFA
                     YVFPAVNHKGEPTEAWLAGLGSSPEEAQEQPEKKAAKLAKRFGKPDTPPSVIPYMQLA
                     IADREKVIGPSTDDRGTLTEPRAFNLMFKTFVGALENESNVAYLRPETAQGIFANFKN
                     VVDTGRVKLPFGIAQIGKSFRNEINPRNFTFRSREFEQMEIEYFCRPDQAQEWYAYWR
                     QARFQWYVDLGLRSDRLRLRDHDSAELAFYSTATADIEYTFPFGQSELEGIAHRGSYD
                     LSQHMKYSGKDLSYFDEERKERFVPHVIEPSAGADRATLAFLCEAYTDDEVGGEPRTV
                     LKFHPRIAPVKAAVFPLVKRDGMPEKAEAIYRELKRHYNVAYDEKGAIGRRYRRQDEA
                     GTPFCITIDSQSLSDETVTFRDRDSCRQWRVPATGVSPALGELLEGQTIPDAT"
     gene            complement(2572..>3111)
                     /locus_tag="VHS97_16935"
     CDS             complement(2572..>3111)
                     /locus_tag="VHS97_16935"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX3449943.1"
                     /translation="LGNSPGEFVPEVCRGKTLVITTTNGTRAILASRKAKRVYIAGFT
                     NLRATSDEISVQFLKKDHGHSVHIICAGTEGHISLEDSLLAGALTSQIVQVTDLAERS
                     HFSDLFGNDEAFMVVTQWLEVERFLEKRPLWKLLSVGRGGQNVLAIGRKPDIDEAAHF
                     DRFNLVAELQLDSLKIVAV"
BASE COUNT          606 a          931 c          918 g          656 t
ORIGIN      
        1 caccgcggac gccatactca gggccctccc cgtgctgcta ggccggtgga gtgtgaatgg
       61 cccagaacgc gattgagcgc ctgatagccc agttcggcga cgcggtcggg ggcgacctgc
      121 cagagatggg gattgagcac ctcgagtgag acatagccgt cgtaaccaat tcgctccagg
      181 tgctcgaaga tcgggccgag ctcgaaatct ccctctcccg gcagaattcg gtcggcgtcg
      241 ccggccagct cgcgcggggt gccgctgaca tcacagactt gcacccaggc cagattttgc
      301 ggcgatagat gggccagatc ctcgaactta ctgggccctg tgtagtaatg gaatacgtcg
      361 agacacaccg ccgcgttgtc ggctcccgat tgagcgatca aggcgatcgc cgtttcgagg
      421 caggcgcaga ggggagacga cttttgaaat tcaatcgcga tccggacgcc gaacgacgcg
      481 gcgagttggg cggcttcacc gagcgcctgg gctgcgcggg catattccgc gatgcccggc
      541 gatcgcacga agtcgggggt gatgatcagc gtgggaacgt tcagttcttg caggagcaac
      601 agacggcgcc ggaagtgatc ccagtgagcg attcgctccg cgccttgaga caggagtaaa
      661 cctccttgag acgccgcggc gaccggcttg agcccgctgg aatcgataag gtcgagggcc
      721 tccgccggtg aatgagtctc gaggaaggat tcgagctttg tcagccagag ctcaacggcc
      781 gtccagccgt agcggccata taaggatagg tccgcttcga acggattttt cagggtcgtc
      841 gcttggctaa tgcaggcttt catgatacgg gtccaggacc cattaattcc tccggctggc
      901 cggctggtca ggtcgaatgt tcggcatttg agctttgaat ctacgcggtg gcggctcgtc
      961 gttcttgcgc cggtccgcag cttcaagtgg cgtcgggaat ggtctgccct tccagcaact
     1021 cgccaagcgc cggggagaca cctgtcgccg ggacacgcca ctggcggcaa ctatcgcgat
     1081 cgcggaacgt gacggtttca tcactcaagc tctggctgtc gatggtgatg caaaacggcg
     1141 tacccgcttc gtcttgccgc cgatagcgcc ggccgatcgc ccctttttca tcgtaagcga
     1201 cattgtaatg ccgtttgagc tcgcggtaga tcgcctcggc cttttcgggc atgccgtcgc
     1261 gcttgaccaa cgggaagact gccgccttga ccggtgcgat tcgtggatgg aatttcagca
     1321 ctgttcgcgg ctcacccccc acctcgtcgt cagtataggc ctcgcagaga aatgccagag
     1381 tcgcgcggtc cgcgccggcg gacggttcga tcacatgagg cacgaaccgc tccttacgtt
     1441 cttcgtcgaa atagctcagg tccttgccgc tatatttcat gtgctggctg aggtcatagc
     1501 tcccccgatg agcgatcccc tccagctcgc tctgtccgaa cgggaaagtg tattcgatgt
     1561 cggcagtggc cgtcgagtag aacgccaact ccgcgctatc atggtctctc agacggagtc
     1621 gatcggaccg aagccccaaa tcgacgtacc actgaaaccg cgcctgccgc caataggcgt
     1681 accattcctg ggcctggtcc ggccgacaga agtattcgat ctccatttgc tcgaactcgc
     1741 gcgatcgaaa ggtgaaattg cgaggattga tctcgttgcg aaagcttttg ccgatctggg
     1801 caatgccgaa gggaagcttc actcggcccg tgtcgacgac gttcttgaaa ttcgcgaaga
     1861 tgccctgtgc cgtctcaggg cgcaagtatg cgacgttcga ctcattttcc agggccccga
     1921 caaacgtctt gaacatgaga ttgaaagcgc ggggttccgt caaagtgcct cggtcatcag
     1981 tcgacgggcc gatgaccttt tcgcgatcgg cgatagcaag ttgcatgtag ggaataaccg
     2041 aggggggcgt gtcgggtttg ccgaaccgct tggcgagctt ggcggccttt ttctccggtt
     2101 gctcttgagc ctcctcgggc gaggaaccca ggcccgccag ccaggcctct gtcggctccc
     2161 ctttgtggtt gaccgccgga aaaacgtagg caaacacgta gagctggtcg gcgcggtagc
     2221 gctctttggt cgcgcggcaa tcgaccatcg ggtcggaaaa gcccccgaca tgccccgagg
     2281 cttcccaaac gcgtgggttc atgatgatcg aacagtcgag cccgaccatg tcatcgcgca
     2341 tccggacgtt gtcgcgccac cacgcgtctt tgatgttgcg cttgagttca acaccgagtg
     2401 ggccgtagtc ccagaagccg ttgattcctc cgtaaacctc gctcgagggg aatataaacc
     2461 cgcggcgctt gcacagtgag accagcttat ccatgtcttc aagcatatcc ctgcctttcg
     2521 ttcaggcgtg accttggcat ggcccgcccc gtacttccgc tctttgccgc atcacacggc
     2581 cacgattttc aacgaatcga gctgcaactc ggcaaccaga ttgaacctgt cgaagtgggc
     2641 cgcctcatcg atatcgggtt tccgcccaat tgctaacaca ttttgcccac cccgccccac
     2701 gcttaatagc ttccataaag gacgtttttc gagaaagcgc tcaacctcca gccactgggt
     2761 taccaccatg aacgcttcgt cgttgccaaa aaggtcggag aaatgagacc tctccgccag
     2821 atccgtaacc tggacgatct ggctcgtcaa ggctccggct agcaaactat cctctaaact
     2881 gatgtggcct tcggtacccg cgcaaatgat gtggaccgag tggccatggt ctttcttgag
     2941 gaactggacc gagatttcat ccgatgtcgc ccgcaggttt gtgaagccgg cgatgtaaac
     3001 acgcttcgct tttcggcttg cgaggatcgc gcgagtgccg ttagttgtgg tgatgaccag
     3061 cgtttttccc cggcaaacct ccgggacgaa ctcgccgggc gaattgccaa g
//
LOCUS       DATAQL010000980         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2237300, whole genome shotgun sequence.
ACCESSION   DATAQL010000980 DATAQL010000000
VERSION     DATAQL010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237300"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..>2807
                     /locus_tag="VHS97_16940"
     CDS             230..>2807
                     /locus_tag="VHS97_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449944.1"
                     /translation="MTIISRFGRPAVVAGLIFSIGISASAQQKTPVPSFTGERVIVAD
                     VPDRYGVLADQIKRLEKSSPQSYYVVVVKSTGTGPWATRLYADELLESWERQRSKRSQ
                     SFDPDRSVVIVVALENEQVAVKPGAFLRNELGLDRDRVERDLLKAKGGFLDLAKENRY
                     TDAISTLLTSTNNWIAGRDSRTSYAPVQITAAKTPGSARPAQTTTTPPAILPNTSSNQ
                     PVERDQPAERDRSSTQTPIAPLKQASSDWLPVVLVGIPVLLIALAIIAGVWLTFRRNQ
                     NRVAGRIKEIKSKAVDVMDRLDSLKERLKLMPTSTDFKQPMTGETQRLYNTVNEKLGT
                     LWDGWLHVMEVLEKAQKLAARSGSPLSQKTLAEAEELITKQGSFEAIEKQAQSITVDV
                     DKLDHAHQEARTVLAVVTLTRPKIDTRLDEIKKLRLATAPYQDELSGVDAGMKEAGTV
                     LVADPLGTMTVLEQLRTRSDALAGRLERVVAIFGDAQKAKSALDTLKRQVAGHRAQGL
                     NLREEGANPDQFLGQGEQSQAETVAALEAGDPQAAAGKLETATSLTNEAQATIEKVQK
                     ARALCERDLPARARETDRLRAALPQAESYQNDLERDFARSSWQAVSRNLDQARALVAT
                     FDDQARQAAAAATISRQDYVKGAAMVEELARQQQIVLRLMSGLGEQLNALINVRNECR
                     KLNEDLAARERQAELLIGQNDSIVSDVARNSLESARRTKGEIQARSGEPRPDWPALRQ
                     SLLEVIDDLALAQSQAEEDLKYHQSLTQEFAQVRNSASRVYAFLASHQEDRLAANQHY
                     QAAADALERVGMEITEPRGRSAVLLEQVRGAAADLKISEGLAREDIRLAAQAQTEITE
                     AGR"
BASE COUNT          585 a          866 c          902 g          454 t
ORIGIN      
        1 cgcggctgta cgctcggccg cggcaaaggc actgaccgcg ctgggtgccg ccgagtgacg
       61 atgccctcgt ggaattgtcc aatgactctc aactcagaag atgacggccc gaccataagc
      121 gtgatattat catcaagcgc ggggcgaccc gagatggaag ccgacggata acagttgtgc
      181 agggatcggc atgtcttgat tgcggaatcg gatacgggca gacctgatca tgaccatcat
      241 ttcgcggttc ggccggcccg ccgtcgtggc cggtttgatt ttctcgatcg ggatctcggc
      301 atcggcccag cagaagacgc cggttccttc atttaccggt gaacgagtga tcgtcgcgga
      361 cgtgcccgat cgctatggcg tgctcgccga tcagatcaaa cggttagaaa agtcttcgcc
      421 gcagtcgtat tacgtcgtgg tggtcaagtc gacgggaacg ggaccgtggg ccacgcggct
      481 atacgcggat gaactgctcg agagctggga gcggcagcga tcgaaacgga gtcagtcgtt
      541 cgacccggac cggtcggtgg tcatcgtggt ggctctcgaa aacgagcagg tggcggttaa
      601 gcccggcgcc tttctccgca acgagttggg tttggaccgc gatagagtcg agcgcgatct
      661 gctcaaggcc aagggagggt tcctcgacct cgcaaaagaa aaccgataca ccgatgccat
      721 ctcaacactc ttgacctcga ccaacaactg gatcgccgga cgcgactcgc ggacgagcta
      781 cgccccggtt cagatcacgg ccgccaagac gccggggtcc gctagaccgg cacagacgac
      841 cacgacgccc ccggcgattc ttcccaatac gtcgtcgaac cagccggtgg agcgcgatca
      901 gcccgcggag cgcgatcgga gttccacgca gacgccgatt gcaccgctga agcaggcatc
      961 gtccgactgg ctgccggtgg ttctcgtggg tattcccgtt ctgctgatcg cgctggctat
     1021 catcgcgggc gtctggctga cgttccgccg caaccagaat cgggtcgccg gccggatcaa
     1081 ggagatcaag tcgaaagcgg tcgacgtcat ggatcggctc gattcactca aggaacggct
     1141 caagctcatg ccgacgtcca ccgacttcaa gcagcccatg accggcgaga cacagcggct
     1201 ttacaacacc gtcaacgaaa aactcgggac gctctgggac ggctggctgc acgtgatgga
     1261 agtgctcgag aaggcgcaaa agctggcggc ccggtcgggc tcgccgctgt cgcagaagac
     1321 cttggccgaa gcggaggaat tgatcaccaa gcagggatcg ttcgaggcga tcgaaaagca
     1381 ggctcagtca atcacggtcg acgtggacaa gctcgaccac gcgcaccaag aggcccgcac
     1441 ggtgctggcc gtggttaccc tcacccggcc caagatcgac acgcggctcg atgagatcaa
     1501 aaagctcagg ctggcaactg ccccctatca ggacgagctc tcgggcgtgg acgccggcat
     1561 gaaagaggct gggacggtgc tcgttgccga tccgcttggc accatgaccg ttctggaaca
     1621 gctccggacg cgttctgacg ccctggcagg ccggctcgag cgggtagtcg caatctttgg
     1681 cgatgcacaa aaggccaagt cagcactgga caccctcaag cgccaagtcg ccgggcatcg
     1741 ggcgcaaggc ttgaatcttc gcgaggaagg tgccaacccc gatcagtttt taggccaggg
     1801 tgaacaatct caagccgaga ccgtggcagc gctcgaagcc ggtgatcccc aagccgccgc
     1861 cggcaagttg gaaacggcca cgtcgctgac aaatgaggcg caggcgacga tcgaaaaggt
     1921 ccagaaggca cgggcgttat gcgaacggga tctgccggcc cgggcgcgcg agaccgaccg
     1981 cctccgcgcg gcattgccgc aggcggagtc ttatcaaaac gacctcgagc gcgactttgc
     2041 ccggtcgtcg tggcaggccg tatcccgcaa cctcgaccag gcgcgggccc ttgtggcgac
     2101 gtttgacgac caggcgcggc aggctgccgc ggccgccact ataagtcgtc aagactatgt
     2161 caaaggagcc gcaatggtcg aagagctggc acggcagcag cagatcgtgc tgcgcctcat
     2221 gtccgggctg ggtgagcagc tcaatgcctt gatcaacgtt cgcaacgagt gccgcaagct
     2281 caacgaagac ctggccgcgc gcgagcggca ggccgagctc ttgatcgggc aaaacgattc
     2341 gatcgtgagt gatgtcgccc gcaatagcct tgaatccgcc cgtcggacta aaggggaaat
     2401 ccaggcgcgc tcgggcgagc cgcgccccga ctggcccgcg ctgcggcaga gcctgttgga
     2461 ggtgatcgac gacttggcat tggcccagtc gcaagccgaa gaggatctca agtaccacca
     2521 atcgctcacc caggaatttg cgcaagtccg caattcggcc agccgtgttt atgcgtttct
     2581 ggccagccac caggaagacc ggctggcggc gaaccagcat taccaggctg ccgccgatgc
     2641 cctcgaacgg gtcgggatgg agatcaccga accgcgtggc cgctcggccg tcctgctcga
     2701 gcaagtcagg ggggcggcgg ccgacctgaa aatctcggaa ggtctggccc gcgaagacat
     2761 ccgcctggcc gcccaggcgc agaccgagat cactgaagcc ggccggg
//
LOCUS       DATAQL010000981         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4984468, whole genome shotgun sequence.
ACCESSION   DATAQL010000981 DATAQL010000000
VERSION     DATAQL010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4984468"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..>2255)
                     /locus_tag="VHS97_16945"
     CDS             complement(122..>2255)
                     /locus_tag="VHS97_16945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3449945.1"
                     /translation="VAILIPTRNHEYRLKKLLDSLTATTYQNYQVYIIDNASDEAGTL
                     RYLASLPHKVMRVPNPAGTFNFAAINNTAAAAVTEELLLFLNDDVEVSSPRWLSQMVG
                     WSRLPGVGAVGARLLYPDQSVQHAGIVHGLHDAVVGHAFRPLPRVERGYWNLDRVTRN
                     CIAVTAACLLTPRTLFLDLGGFDEKRFPVAYNDVDYGYRLADAGFRSVYCAEAVLCHH
                     EGLSRGRTDDPRALATFRQIHGRRVDPYFSPHLDPEHEIFEPRPTVVPVSRNGRPVSV
                     LAVIHSLNWEGAPRFEFELLSRLTAAGSIQAEVVSPCDGPMRPAYEQKGVKLRVVPEL
                     ADMATVPRSYEEGTRYLSGLIRDGSYEVVHANTLESFWGIDAARMVGTPSVWSIHESD
                     TWQNYFDDLPREIAATALTCLSHPYRVVFSAKSSADRFSALNSNGNFGLIRFPLDNNR
                     FNSELARIDRRAAREELELKTDDFCVLLLGTVCQRKGQHDLLRAFASLSEDIAARTTC
                     LVVGSRDRLAYSRKLEEMARSLPQDRRHRFRVIPETGDTAAYWRAADVFCCTSRVESY
                     PHVILEAMAAGLPVVTTPVFGIAEQVRPSINSLIYEPGDVRALGRHLALLATDELKRR
                     QFALASPWVLRSLPSHVDVDERYERVFRAAAESTPVIQVVAATPSIPHSRPTAKRILL
                     FDTARFAIRSRKTRAQEAIRRPRVVQNE"
BASE COUNT          485 a          651 c          688 g          431 t
ORIGIN      
        1 aaaaagtaag caagcccagc atccttgccc cgctgcaaag ggcgggaagg gaaaacctgg
       61 tctaattgcc acctcaccca cgctagcatg gcctagcgcc cattccaagt ataggtcacg
      121 cttattcatt ttgaacgact cgcggccggc gtattgcctc ttgcgctcga gtctttcgag
      181 aacggatcgc gaatcgtgcg gtatcgaaca gcaagatacg cttcgcagtc ggccgtgaat
      241 gtgggatcga tggtgttgca gcgacaacct ggataaccgg cgtactctca gcggctgcgc
      301 ggaaaacacg ttcgtaacgc tcgtctacat caacatggct gggaagacta cgaaggaccc
      361 agggtgacgc cagagcgaat tgcctccgct tcaattcatc ggtcgcgagc agagccaggt
      421 gccggccaag ggcgcgcacg tcaccgggct cataaatcaa agagttgatc gagggtcgca
      481 cctgttcggc gattccgaat actggcgtcg tcacgacggg taacccggcg gccatggctt
      541 cgaggatcac atgaggataa ctttcgacgc gagacgtgca gcagaacacg tcggccgcgc
      601 gccagtatgc cgctgtgtcg cctgtctcgg gtatcacgcg aaatcggtgg cgacgatcct
      661 ggggaagcga ccgagccatt tcttcgagtt tgcggctgta ggcaagccgg tcgcgagagc
      721 caacgacgag acaagtcgtt cgggcagcga tatcttctga aagtgacgcg aaggcgcgca
      781 gcagatcatg ctggcctttg cgttggcaga ccgttcccaa aagcaagacg cagaagtcgt
      841 ccgttttcaa ttccagttct tcgcgggccg cgcgacgatc gatccgcgcc agctcggagt
      901 tgaatcgatt attgtcgagt ggaaagcgga tcaacccgaa attgccgttc gagttcaaag
      961 cactgaagcg atccgcggag ctcttggccg agaatacgac tcgatagggg tgcgacagac
     1021 atgtgagggc ggtcgcggcg atctcgcgcg gcagatcgtc gaaatagttt tgccacgtgt
     1081 cgctctcgtg aatgctccat accgacggcg tccctaccat gcgtgcagcg tcgatccccc
     1141 aaaatgattc cagcgtattg gcgtggacga cctcgtaact gccgtcccgg atcagcccgg
     1201 acaggtaccg ggttccctcc tcgtaagatc tcggaacggt cgccatatcg gccagctcag
     1261 gtacgacccg gagcttgact cccttttgct cgtatgccgg gcgcatgggc ccgtcgcagg
     1321 ggctgacgac ttcagcctgg attgatccgg cggctgtcaa tcgtgaaagg agctcgaact
     1381 cgaaccgtgg tgctccctcc cagttcaagc tgtggatcac cgccaggacc gagacgggcc
     1441 ggccgtttcg tgagaccggg acgaccgtcg gtcggggctc gaagatctca tgctcaggat
     1501 cgagatgtgg actgaagtaa ggatcaaccc ttcggccatg aatctgcctg aacgtggcca
     1561 gggcgcgagg atcgtcggtc cggccgcgag agagtccttc gtggtgacaa agcacagcct
     1621 cggcgcaata gacgctacga aaaccggcat cggcgagccg atacccgtaa tcgacgtcgt
     1681 tgtacgcgac gggaaaccgc ttttcgtcga accctccgag atcgagaaag agggtgcgcg
     1741 gagtgagtag acacgcggcg gttacggcga tgcagttgcg cgtcacgcga tccaggttcc
     1801 agtaaccccg ctccacccgg gggagaggac gaaacgcatg gccgaccacc gcatcgtgaa
     1861 gaccgtggac gatgccggca tgctgcacgc tctgatcggg atacagaagg cgcgcgccga
     1921 ccgcccccac gcccggcagc cgcgaccaac ccaccatctg gctcagccac cgtggactgg
     1981 acacctcgac atcgtcgttc aagaacagaa gcagctcttc ggtgaccgcc gccgcggccg
     2041 tgttgttgat cgcggcgaaa ttgaatgtgc ccgcgggatt ggggacgcgc atgaccttgt
     2101 gtggtaacga cgccagatag cggagcgtcc cagcttcatc gctggcgtta tcaataatat
     2161 agacttgata gttttgataa gttgttgcgg ttaacgagtc cagcaatttc ttcaggcgat
     2221 attcatggtt ccgtgtgggt atcagaatcg cgacg
//
LOCUS       DATAQL010000982         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4527038, whole genome shotgun sequence.
ACCESSION   DATAQL010000982 DATAQL010000000
VERSION     DATAQL010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4527038"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VHS97_16950"
     CDS             complement(<1..1026)
                     /locus_tag="VHS97_16950"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX3449946.1"
                     /translation="MIDAKPEPLDSLPVLPLKNSVLFPHLFLPLSIGRPVSLAAAEAV
                     LATEDKTFLALALKNPQEDPPAADDLYTIGTKAVIKKMARSGAGIELLVQGLERVTLV
                     RLEQTEPYLRAKVRPLPLPEDQGTEVEALRRAVLDLTARALELAHPEGGVNIDQLAAQ
                     ATDPLILVFLIGSMLSLDVPREQALLETTSRAEGLRMLHGYLTHELQILELRQKISSQ
                     AQNEMGREQKEYLLRQQLRAIQGELGETTPEKADVQELRNRLLDADLPEDVHKEFERE
                     LGRMERLPAAAPDYQITRTYLEFVLELPWRKSTEDDLDLNRAQQVLDEDHFDLEKIKE
                     RIIEHLAV"
     gene            complement(1044..1409)
                     /locus_tag="VHS97_16955"
     CDS             complement(1044..1409)
                     /locus_tag="VHS97_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3449947.1"
                     /translation="MAADKINFPHVFFLPTTRPADEVWEPRADVYHMRDGWLIKLELA
                     GVKLDDIHLVAHGPTLLVQGTRRDEHCHQGTGCHCMEIAYSQFQRVIELPGLSTRPEM
                     AMSYVDGMLLVRIKAGGSH"
     gene            complement(1698..2924)
                     /locus_tag="VHS97_16960"
     CDS             complement(1698..2924)
                     /locus_tag="VHS97_16960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3449948.1"
                     /translation="MREECAYLTEEIDRRSFLATGAGALAAAAALGDGQTADAQGTTR
                     QTAELPKRSLGRTGTKVTMLCLGTWMSPGGGRLLRTAWANGVRYVDTAKSYGSEPMIG
                     AWMNTVPVPRNELFLVTKDQPNTPAQLIRQLDQRLQSLQTDYVDLIFLHALGDRNFEV
                     ELQWPMSQEFKETADAIRKSGKAKFVGFSTHHPDRALLLQAAVQGGFVDAIMLQNNPW
                     IAQDDNMNRALDACYKRGIGLISMKQVAGNMNLEEIGMQLPDLTAQGLTPYQALLHAI
                     WTDERFSSVCVSMRNTDQIRVNAAAARVFKPMKKVEIERLRDACIAAGPTMCASCDGR
                     CSRAAGTTAELGNLTRFLTYHDHHGYRAHARRLYAELGDVARDWRGADLEAARQACPN
                     RLDFAKLLPRAQEVLG"
     gene            3202..>3462
                     /locus_tag="VHS97_16965"
     CDS             3202..>3462
                     /locus_tag="VHS97_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3449949.1"
                     /translation="MFPQASKQCGKKGFTLIELLVVIAIIAVLIALLLPAVQAAREAA
                     RRIQCVNNMKQIGLAIANYESTNGCIVSGYISSTVPLSLFGVP"
BASE COUNT          627 a         1029 c         1079 g          727 t
ORIGIN      
        1 gaccgcgagg tgttcgatga ttcgttcctt gatcttttcg agatcgaagt gatcttcatc
       61 gagcacctgc tgcgctctgt tgaggtcgag gtcgtcttcg gtcgatttgc gccaggggag
      121 ctcgagaacg aactcgagat aagtccgcgt gatctggtag tcaggagccg ccgcgggtag
      181 gcgctccatc cggccgagct cgcgttcgaa ttccttgtgg acgtcctcgg gaagatcggc
      241 gtcgagcagg cgattgcgca gctcctggac atcggctttc tcgggagtgg tctcgcccag
      301 ctctccctgg atggcccgga gctgttgccg cagcaagtac tccttttgct cgcgtcccat
      361 ttcgttttgg gcctggctgg aaatcttctg ccgcagctcc aagatctgca gctcatgagt
      421 caggtaacca tgaagcatcc gcagcccctc ggccctgctc gtagtttcga gtagggcctg
      481 ctctctgggc acatccagac tcagcatcga accgatcagg aataccaaga tcagcggatc
      541 agtcgcctga gcagcaagtt gatcgatatt gactccacct tcggggtgtg ccagttcgag
      601 agcccgggca gtcaggtcga gcacggcccg gcggagtgcc tcaacctcgg ttccttggtc
      661 ttcaggaagc ggcaacgggc ggactttggc cctgagataa ggctcggtct gttcgagacg
      721 gaccagggtt actcgctcca aaccctggac caagagctcg atcccggctc cagaacgcgc
      781 catctttttg atcacggcct tggtgccgat ggtgtagagg tcgtcggccg cgggcgggtc
      841 ttcctgaggg tttttgaggg cgagggccag aaacgtcttg tcctcggtcg cgagaactgc
      901 ctccgccgcg gccagcgaaa cgggacgtcc gatcgagagc ggcaagaaca ggtgaggaaa
      961 aagaacggag tttttgaggg ggagcaccgg cagagaatcc agaggctccg gtttcgcgtc
     1021 gatcattagc tcgttttcgc ttttcaatgg ctcccccccg ctttgatccg caccaagagc
     1081 atgccgtcga cgtaggacat cgccatttcg ggcctcgtcg agagccctgg taattcgatg
     1141 actcgctgaa actggctgta cgcaatttcc atgcagtgac accccgtgcc ttgatggcag
     1201 tgctcgtcgc gtcgcgttcc ctgtacgagc agcgtgggac cgtgcgcgac gaggtggatg
     1261 tcatcgagct tcacgccggc gagctcgagc ttgatcagcc agccgtcgcg catgtgatag
     1321 acatcggccc gcggttccca gacttcgtca gccggcctgg ttgtcgggag aaaaaacacg
     1381 tggggaaaat tgatcttgtc cgctgccatc ggacctcccg atttggtgtg cggatcaccg
     1441 cttcgccgta ccgtgtcact actctgtgat gatcctccgc gagccgttca gttgcaagaa
     1501 ccgaccttgc aaggccgggc ccgcgcgcca ctggcaagac ggccgcaaat ctggcagagg
     1561 ttcaaggtga taacgcgcct tacttgaacg gccacgcctg cgttcaccac ggtggaagaa
     1621 aacgaccggt tcgcgccgcg agttgtggcg gcgaaccggt cgtgccaggt acgaacttga
     1681 tggcaccgcc tgccgggtca gcccagtacc tcctgggcgc gcggcaagag cttggcgaag
     1741 tcgagccggt tcgggcaggc ctggcgggca gcctcgaggt cggcgccgcg ccagtcacgc
     1801 gcgacgtcgc cgagctcggc gtagagccgc cgggcatggg cgcggtagcc gtgatggtcg
     1861 tggtaagtga ggaaccgggt caggttgccc agttcggcgg tcgtacccgc ggcccgactg
     1921 catctcccgt cgcaggacgc gcacatcgtc gggccggcgg cgatgcaggc gtcgcgcagc
     1981 cgttcgatct caaccttttt cattggtttg aagacccgag ccgccgcggc gttcacgcgg
     2041 atctggtcgg tgtttcgcat cgagacacag actgacgaaa accgctcgtc ggtccagatc
     2101 gcgtgcagca aggcctggta gggtgtcagg ccttgcgcgg tcaggtcagg gagctgcatg
     2161 ccgatctctt cgaggttcat gttgccggcc acttgcttca tcgagatcag cccaatgccc
     2221 cgcttgtagc aagcgtccaa ggcgcggttc atgttgtcgt cttgcgcgat ccaggggtta
     2281 ttctggagca tgatggcatc gacgaacccg ccttgcacgg cggcctgcag gagcagggcc
     2341 cgatcgggat gatgggtcga gaacccgacg aacttcgcct ttcccgactt gcggatcgcg
     2401 tcggcagtct ctttgaactc ctggctcatg ggccattgca attcgacctc gaagttccgg
     2461 tcgcccaggg cgtggaggaa gatcaagtcg acgtagtcgg tctgaagcga ttgcagccgc
     2521 tggtccagtt gccggatgag ctgcgcgggc gtgttcggtt ggtccttggt taccaggaac
     2581 agttcgttgc gaggcacggg caccgtgttc atccaagcgc cgatcatggg ctcggagccg
     2641 tagctctttg ccgtgtcaac gtagcggaca ccgttggccc aggcagtgcg gagcaaccgc
     2701 ccgccccccg ggctcatcca cgtgccgagg cagagcatcg tcactttagt gcccgtacgg
     2761 cccagcgacc gcttgggcaa ctccgccgtt tgtctggtcg tgccctgggc gtccgcggtc
     2821 tgtccgtcac cgagagctgc agcggcggcc aacgcgccgg ctcccgtcgc caggaaggag
     2881 cggcgatcaa tttcttcggt cagatatgca cactcttccc tcataatgcg tctcctggtt
     2941 gtcttacgac agatggatgt gatgtcccca gcgggtattc gcgaaatgaa gaacactgac
     3001 ggcaccctaa tcgccgccgc ccgcgttgtc gaggaaaatc cgcgctccgt gtgaaactcc
     3061 gacaaaccaa gcgtgggcac gggggattgg cgcgactaat tcccaatttt gggccaccaa
     3121 aaagatctat tgtatttaac gaatatagtc gttagccttt ccgatgtctt tcatttcgaa
     3181 aactgactgg aaaggcagcc catgttccct caagccagta agcaatgtgg caagaagggg
     3241 ttcaccctga tcgagttgct ggtggtcatc gcgatcatcg cggtcttgat cgccctgctt
     3301 ttgcctgctg ttcaagcggc ccgcgaggcg gcacggcgga tccagtgcgt caacaatatg
     3361 aagcaaattg gcctggcgat cgccaactac gagtcgacca acggctgtat cgtctcggga
     3421 tacattagct cgacggtgcc cttatcttta ttcggcgtcc cg
//
LOCUS       DATAQL010000983        14812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4984507, whole genome shotgun sequence.
ACCESSION   DATAQL010000983 DATAQL010000000
VERSION     DATAQL010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 14812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14812
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4984507"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..1419
                     /gene="pheS"
                     /locus_tag="VHS97_16970"
     CDS             421..1419
                     /gene="pheS"
                     /locus_tag="VHS97_16970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX3449950.1"
                     /translation="MDARVLAVSQEVSALADAALKLINRASTIDELEKLRVAYLGKQS
                     KIMLLSKELGKVPPDLRPAFGEAVNAGKKRLQEALAERKALVEPAGRRSKSAIDVTLP
                     GIRKGVGRRHPLMQTMEAVKEVLQGMGFRYDDYPEVETEYYNFDALNTPRWHPSRDMH
                     DSFYTKGGGVLRTHTTAFQVRAMRQQQSPPIRAMTSGRCYRRDEIDATHFPIFNQLDV
                     IAIDEAISFADLKWTLERLIRTLLGQDVKLRFRPSFFPFTTPSAEVDVFYRGRWMELL
                     GSGMIRPEVLKNGGLDPDLCQGFAFGMGIDRLTMARYGIDDIRLLYANEESFLTQF"
     gene            1438..3894
                     /gene="pheT"
                     /locus_tag="VHS97_16975"
     CDS             1438..3894
                     /gene="pheT"
                     /locus_tag="VHS97_16975"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX3449951.1"
                     /translation="MPMIVNTAWLLEYLDPKCSHDELLDALPRLGLEVEQTHELKTEL
                     DAVRIGFVRRKQPVPEARGYYLCEIELERGRMIPVVCASEHEIREGWGVPVAVAGTVL
                     PTGRSIKSAPFHGVRSEGMICLDGELGMVARGTGMHHFTDESVLGAPLPKAVDVPEYL
                     LELNVLPNRPDFLGLIGIAREVAALLRLELRYPAVLAPRGGHANPPVTVEIREPDLCP
                     RYMCGLVTGVKVAPSPPWLKARLLLAGMRPINNVVDITNYVLYEWGQPLHAFDFRKIT
                     GSRIVVRRIAPGETLELLSGAVVGASGQPGRTKFSEPPLVIADQERPVALAGIMGGRS
                     TQTAEETTQVLVEAAHFDPIMIRRTVQQVDLGIEGRGTASSYRFERGTDPNLMLEGAL
                     DRALQLIAEIAQGTPVGQDVDQYLRPRERRIFRLSTARTSSYLGMPVEVGTVRDCLTR
                     LGMECTGNEHEVQVSVPTWRADVNDPVVLIEDVARMVGYDQIPVSPRPSLPSLGLRST
                     SDRIRQAVAAQLVSAGFLECRNPSLESLKMSAWLGETDDAITLSNWATREMSVLRRTL
                     LAGLAATVQTNIRRGADSVWFFEVDRLFVPLATKPDDSGSMAGRWHVAGIAGGRHHRS
                     DWRSDGTQADFFTLKGVVEDLLETLGARNVAFHPTDRKPFVAGTSAEVSLAGAGLVGS
                     IGELDPKSVEFERVPFRVYCFELDLESLEETFKTLPAYHQLLRQPAVARDLAVVARVE
                     VSYAELVDTIRSTAGPTLENLRLVDQYQGAQVPPGHRSLAFHMLFRDPERTLTAEEVA
                     ETVARIVAALKDRFGADLRA"
     gene            complement(3994..4242)
                     /locus_tag="VHS97_16980"
     CDS             complement(3994..4242)
                     /locus_tag="VHS97_16980"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX3449952.1"
                     /translation="MHIILFLLIGIAAGWLAGQLVKGRGFGLVENLIIGVIGALIGGF
                     VFGALNVNVGGLLGQLIAATVGAIILLYLLKLLRRHSW"
     gene            complement(4345..6297)
                     /locus_tag="VHS97_16985"
     CDS             complement(4345..6297)
                     /locus_tag="VHS97_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3449953.1"
                     /translation="MRRRKNGEFKGIRQELATIGRQASEVWRLIPGRHKMSLAGALAI
                     MSLASGANTAIALCVGRLIDAVNPYEHSSQSVADLTRIAAVYLAIIGGAYLVREVMNV
                     LRRFLVEDTCTRIDRDMYVRIVSHLLKVDLGVLAHDQVGGLYGRITRGVDGLVRFLRV
                     GFLDFVPALFMGTFALSTTLAKQPRIALAMAGVIPVSLGLTIWQLITQKGIRLDLLAS
                     REAMDGTVVEQLGGIDYIRAANTDRLEVERVERAAEYRRAKELRHHFEMSLFGSGKAI
                     NEGFFHVVVLAFAIYLFVHGGLKSGEIFTFSMLYLNVMAPLNEIHRFIDEAHESSLRV
                     GDLLGLLAEPLDRSFELSAADEPRLVVGEPVFVTDQLCVEYRDADGAAKPALNGLSSL
                     IRHGETIGVAGRSGCGKTTWLRTMMRLAHPTGGRATLGGVELSSISRQSIGELVGYVG
                     QNPFVFAGTIAENIAYGCESATEDQIRRAAERACIHREIMALPGAYSARVAERGQNLS
                     GGQKQRIALARVFLKNPPILILDEGTSALDNISERLVQRAITAARADRTVILVAHRLS
                     TLREADRILVFDGGKIVAIGTYNELVRQGGVFAELVRSAEEADAASEAQPPSPSGSTP
                     IKLEDLAENGQPGPLVTAPIVTSPDR"
     gene            complement(7097..14635)
                     /locus_tag="VHS97_16990"
     CDS             complement(7097..14635)
                     /locus_tag="VHS97_16990"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX3449954.1"
                     /translation="MARKAVELAVQGRGSGEPRDHVGFYLIDRGQQELMSAFRFRPTW
                     RERLLDLVLGRPQSVYFGSIVIGLAALLALVVIFALGGSLTNFWWILAIAAAALLPLS
                     ELVVGLVNQLFTLFVPPRVLPKLDVKHGIPTEHTTFVVIPSLLARPASGAVLLERLEL
                     HYLANPDPSLRFALLTDFTDAPRETMPQDEGIIVDALERVRALNDRYAPEGTNLFFLF
                     HRRRLWNEAQGCWMGWERKRGKLLEFNRLLRGARDTSYAVLSADPAALPRIGFVITLD
                     ADTQMPRDTAGRLVGTLAHPLNRPRLDPNQERVTTGYGVLQPRISFHLAAATHSRFAA
                     LLATAGGIDPYSTAASDAYMDLFGVGSFTGKGIYDVDAFEAATGSRFPENHILSHDLI
                     EGNFARCGLISDTELFDDFPALYHAYARREHRWVRGDWQLLPWLGRRVPTPKGWRPNP
                     LPLLERWKLADNLRRSLVPPALLVLLLLGWTVLPGSPWLWSGVALATLGLPLIQSIIG
                     SAVESVRGRSLAGLYTGLFGTPAVIGQVLLDVTFLAYRGVLLLDAVIRTLVRLLWTRR
                     KLLEWETAASTELRLKGGLVQFVTGMWVAPALAIAMGTVVLLVRPAALPAAGPFLFAW
                     LLSPAVAFRVSQPRPIAQISLADDDRRALRRIARKTWQFFEIFVGEDDHWLPPDNFQE
                     VPDGRIAHRTSPTNTGLLLLSTLAAHDLGYIGLSTLIDRLERTFDTFDRLEKHWGHFY
                     NWYDTRTMQPLSPRYISTVDSGNLLGCLIALKQGLLEKCEKPVVGPTVIEGLADTVFL
                     VAEETGEDCRRLKALLDVTPGDLAEWDSWLEEVEGAAVDLAARTDAKDSAAAATKWEA
                     GGWTGRLVEQVRSWRAEVAAAVAESRTKEWLSRLRHLADRAEDLAATMDFRPLYKPDR
                     HLFAIGFNLAQGKLDNACYDLLASEACLTSYLAVARGEAPRRHWFQLGRHFISAGGKI
                     GLLSWGGTMFEYLMPRLLLRSLPGTILAEACQTAVTRQIEYGRQLGTPWGVSESAFSA
                     QYPDGDYQYQAFGVPGLGLKQGLEKEQVVAPYATVLAALLVPHEALDNIRRLAQEGAD
                     GPFGLYEAIDYTRGRIPADERRVVMRSYMAHHQGMSLVALANTLLNDVMPKRFHAEPL
                     VRALELLLQERLPSDPEIIETTDAKLTASTPSAADKVGPLAPMSRRLTSPATPAPRTN
                     LLSNTQYHVMITNAGSGYSICRGMDVTRWREDPTCETWGQFCYIRDLQRDLVWSAGYQ
                     PVCKRADEYEVIFAADKSTIRRRDGEIETLIEVTVSPEQPVEVRRVTLTNHDSRPREL
                     DLTSYSEVVLAPHRDDLAHPAFGKLFLETEWVASSGALLCRRRPRSVDEPPICAVHVA
                     AVEVNAAGSATVGERQYETDRCRFIGRGRTVANPAALDSGSVLSGTVGPVLDPVFCLR
                     QRIRLKPGGSATIALATAVASTRAEGLALADQYRQASAASRVFELAWAHSQIEHRHSD
                     RFGESAHLFQRLAAHVLFAGSALRADRQILASNTLGQEGLWTFGISGDRPIVVARFGA
                     TDEIHLARELLAAQTYLRGKGLELDLVLLNDQPAGHEEELSRQLMSVVRTDGGSDRIG
                     QPGGIFLLKAAELPEDGKILIQAAARAVLVGDGGSLASQLDRTELRHPLPGPLTMSHE
                     HPRWQDEPVTLPSDLRFPNGLGGFTPDGREYCLLIRSDDRAHSRGNGQPVSHAVLYPR
                     LAPAPWINVVANPGFGFLISESGSGYTWSQNSQANRLTPWSNDPVADPPGEIVYLRDE
                     ENGDIWSPTPLPAPSSQPTLVRHGQGYTTFEQNAYGLFHELTLLVAADDPVKLIVLRI
                     QNLSDRPRRLSATYYAEWVVGSTRDATAMHVVTEVDPETGALLARNTFRTDFGSRVAF
                     ADVDRRPRSFTADRAEFVGRHGSMMAPAALGRVELSGATGEALDPCAAIQVAFSLEAG
                     AKTQLVFLLGEAEGPDAARALIKRYREEGRAASALSDVKARWDELLETVQVRTPDPAL
                     DLLTNRWLLYQVLSCRVWGRSAFYQSGGAYVFRDQLQDVMALFHAAPDLARTHILRAA
                     ARQFPEGDVQHWWHPPKGRGVRTRIADDPLWLVFVTQRYVTVTGDSAILDEPIPYLNG
                     PPLQPGQDDDYGLPKPGAESRPLYEHCLRALEYTQRLGAHGLPLMGHGDWNDGMNRVG
                     SRGKGESVWLTWFSIICRIRFAAIALERGDDERSAELRRQADELRLTVEESAWDGEWY
                     RRAYFDDGTPLGSSQNSACKIDSITQTWAVLSGGGSPERQHLAMQAVNDLLVREADRL
                     ILLFDPPFDGDALDPGYIKGYLPGVRENGGQYTHAATWVVEAAARQRQGQRAYQLLSI
                     INPVLHADDPNGVERYKVEPYVVAGDVYSRAPHVGRGGWTWYTGSAGWFYRVFVESIL
                     GIERVGDRLAFNPCVPPDWSHFEMTYRLRSTTYLIKVQNPAGVESGVAAVWLDNARQP
                     GNGLRLTSDGKNHEVRVVMGALNETDGLASLQ"
BASE COUNT         2949 a         4521 c         4510 g         2832 t
ORIGIN      
        1 gtcgcttaac cttcatgctc caaccagatg cgtcatcagg ccgtcttctc gtctcggctg
       61 tttttcgatc caggaatcgt tcctgaaaat acacaagccc cgagcgaaga cttgaatgct
      121 ttatgggtcg ccgtctcaat ctatactgcc ccgggaccgc ggatttcttc cattattttg
      181 ttgggagaca attcggtttc ttcagatttc gccaccaaga ccctgggatc aacccaactt
      241 actgcccgat cagattcaag gttactctgg cttgatgatc ccgtcagaag tacgatgagg
      301 atcccctgag ggaagggtgc gataccaagc ctggcacaag ctcatcgaac ccaagctggt
      361 taattcttgt atactaacgg gtctgatatc ggaatgacgt ccgaggaaaa aacggaaacg
      421 gtggacgcga gggtgctcgc ggtctcgcaa gaggtgtccg cgcttgccga cgccgccctg
      481 aaactgatca accgagcctc cacgatcgac gagctcgaga aactccgcgt ggcgtacctg
      541 ggaaagcaaa gcaagataat gctactttcc aaggagctgg gcaaagtccc tccggatctg
      601 cgtccggcgt ttggcgaagc ggtcaacgcc gggaagaagc gcttgcagga agcgcttgcc
      661 gagcgcaagg cacttgtgga acccgcgggc cgtcgctcca aaagcgcgat cgacgtgacg
      721 cttccgggaa ttcgcaaagg ggtcggcagg cgtcaccccc tgatgcagac gatggaagcg
      781 gtcaaagagg ttctccaagg gatgggattc cgctacgacg attatcctga ggttgagacc
      841 gagtactaca acttcgatgc gctcaacacg cctcggtggc acccctcgcg cgacatgcat
      901 gattcgttct ataccaaagg cggaggcgtg ctgcgaacgc acacgaccgc ctttcaggtg
      961 cgggcgatga gacagcaaca gagcccgccg atccgcgcga tgacttccgg ccgttgttac
     1021 cggcgtgacg agatcgatgc gacccatttc ccgatcttca atcaactgga cgtgatcgca
     1081 atcgacgagg cgattagctt tgccgatctc aagtggacgc tggaacgctt gatccgcacg
     1141 ctcttggggc aagatgtcaa gctccggttt cggcccagct tcttcccgtt caccacgccc
     1201 agcgctgagg tggatgtgtt ttaccgcggt cgatggatgg agctcttggg ctcgggcatg
     1261 atccgcccgg aggtcctcaa gaacggtggg ctcgaccccg atttgtgtca gggattcgcg
     1321 tttggaatgg ggatcgatcg actcaccatg gcgcggtacg gaatcgacga catccggctc
     1381 ctctatgcca acgaggaatc ctttctcacc cagttttgat cgcgttcgag tttcccgatg
     1441 ccgatgatcg tcaacacagc gtggcttctc gaatatctcg atcccaaatg ctcgcacgat
     1501 gagctgctcg atgccttacc gcgtctcggc ctcgaggtcg agcaaacaca cgagctcaaa
     1561 acagagctcg acgcggttcg gattggtttt gtgcggcgga aacagcccgt gcccgaagcg
     1621 cgaggatatt atctctgcga gatcgagctc gaacgaggcc ggatgatccc ggtggtttgc
     1681 gcctctgagc atgaaattcg cgaaggctgg ggtgttccgg tcgcggtcgc tggaacggtc
     1741 ttgccgactg ggcggtcgat caagtcggcg ccgtttcacg gggtcaggtc ggaaggaatg
     1801 atctgtctgg atggcgagct gggaatggtg gcccgcggga cgggcatgca ccatttcacc
     1861 gacgagtctg tgttgggcgc gccgttgcca aaggcggtcg acgtcccgga gtatctgctc
     1921 gaattgaacg tgctccccaa tcggcccgat ttcctcgggt tgatcggcat cgccagggaa
     1981 gtggccgcgc ttttgaggct cgagcttcgt tacccggccg tgctcgcacc ccgcggcgga
     2041 catgcgaacc cgccggttac tgtcgagatc cgtgaacccg atctttgccc gcggtacatg
     2101 tgcggtctgg tcaccggcgt gaaagtggcc ccttctccgc cgtggctgaa ggcccggttg
     2161 ctcttggccg gcatgcgacc gatcaacaac gtcgtcgaca tcacaaacta tgtcctttac
     2221 gagtggggcc agccgctgca cgcgttcgat ttccggaaga taacgggctc acggattgtc
     2281 gttcgtcgca tagcccctgg cgaaacactg gaactcttga gcggcgcggt cgtgggcgcg
     2341 agcggtcagc ccggcagaac gaagttctcc gagccacctt tggtgatcgc cgatcaagag
     2401 cgtccagtgg ccttggcggg catcatgggg ggccgctcga cgcaaacagc cgaagaaacc
     2461 acgcaggttt tggtcgaggc cgcccatttc gatccgatca tgatccggcg caccgtgcag
     2521 caggtcgacc tcgggatcga ggggcgcggc actgcgtcgt cctatcggtt cgagcgaggc
     2581 acggatccca atttgatgct tgaaggagcc ctcgaccgcg cgttgcagtt gatcgccgag
     2641 attgcccagg gcacgcccgt ggggcaagat gtcgatcaat atctccgacc gcgcgagcgc
     2701 cggattttcc gcctgagcac tgcacgcacc agttcttacc tgggcatgcc ggtcgaggtt
     2761 ggaactgttc gggattgtct cacccgcctc ggcatggaat gtaccggcaa cgagcatgag
     2821 gtgcaagtct ctgtgccgac ctggcgggcc gacgtgaacg atccggtcgt tttgatcgag
     2881 gacgttgcac gcatggtcgg ctatgaccag atcccggtct ctccgcgacc gtcgctgccc
     2941 tcgttgggct tgcgctccac cagcgaccgg attcggcaag ccgtcgccgc gcaactggtt
     3001 tcggcggggt ttctcgagtg ccgcaatccg tcgctcgagt ctctcaagat gagtgcttgg
     3061 ctgggagaga cggacgacgc gatcaccctc tccaactggg cgacgcgtga aatgtcggtg
     3121 ctgcggcgta ccctgctggc gggtctggcg gccacggtgc aaaccaacat ccgccggggg
     3181 gcagactcgg tctggttttt cgaggtcgac cgtctctttg ttccgctcgc gactaagccg
     3241 gatgactccg gttcgatggc gggccgatgg cacgttgccg gtatcgccgg gggcaggcac
     3301 catcgctccg actggcgctc cgatggaacc caggccgatt tcttcacgct caagggggtc
     3361 gtcgaagatc tgctcgagac tttaggcgcg cggaatgtcg ctttccaccc gaccgaccgg
     3421 aagccatttg tggctggaac gtcggcagag gtgtcgctcg caggcgccgg gctggtcggc
     3481 tcaatcggtg agctcgaccc caaaagtgtc gagtttgaaa gggtgccatt tcgcgtctat
     3541 tgcttcgagc tcgacctcga gtcgcttgaa gaaacgttca aaactctgcc ggcttaccat
     3601 cagctcctcc ggcagccggc cgtcgcccgc gacctcgccg tcgtcgcccg cgtagaggtt
     3661 tcgtatgccg aactcgtcga taccatacga tccacggccg gccccacgct cgaaaacctt
     3721 cgtctggtcg accagtatca gggggcccag gtaccgcccg ggcaccggag cttggccttc
     3781 cacatgcttt tccgcgaccc cgagcgcacg ctgactgccg aagaagttgc tgaaacggtc
     3841 gcacggatcg tcgctgccct caaggatcgt ttcggggcgg acttgcgcgc ctgaacgtcc
     3901 gttcgatctg agattggcga gcggctcggt caaacgaatt ggcgggggcg aatcgcgagc
     3961 taacggcttg cacgaaggtc agccgatcaa cgctcaccag ctatgccggc ggaggagctt
     4021 gaggagatag agcagaatga tcgctccgac ggtggccgcg atcaattgcc ccagcagtcc
     4081 tcccacattt acgttcagcg ctccaaacac aaagccgccg atcaatgccc cgatcacgcc
     4141 gataatcagg ttctccacca gtccgaagcc tctacctttg accagttgtc cggctaacca
     4201 accggccgcg attccgatca gcaagaataa tatgatatgc acttacccag tctccgatcc
     4261 gatcagcaag aataatatga tatccactta cccagtctcc gagttgagtt ccgcgccgct
     4321 gggagtgtga gtgtgtgtgg atccttatcg gtccggtgaa gtcacaattg gtgcggtgac
     4381 cagcgggccc ggttggccgt tttccgccag gtcctccagc tttattggag ttgagcctga
     4441 gggcgaaggc ggttgcgctt cggaagcggc gtcggcctcc tcggctgatc ggaccaactc
     4501 ggcaaagacg cccccctgcc gcacaagttc attgtaggta ccgatcgcga cgattttgcc
     4561 gccatcgaaa accagaatgc gatcggcttc gcgaagagtg ctgaggcgat gcgcgacgag
     4621 aatgaccgtg cggtcggctc gggctgcggt gatggcacgt tggaccagac gttcgctgat
     4681 gttgtcgagg gcggacgtgc cttcgtcgag aatcaagatc ggcgggtttt tgagaaagac
     4741 acgcgccagg gcgatccgct gtttttggcc tcccgagaga ttctggccgc gttccgcgac
     4801 tcgggcgctg taagcacccg gcaatgccat gatctcgcgg tgaatgcagg cccgctccgc
     4861 cgcgcgtcgg atctgatctt cggttgcact ttcgcagcca taggcgatgt tttccgcaat
     4921 cgtacccgcg aagacgaacg ggttttgacc gacatatccg accagttcgc cgattgactg
     4981 gcgtgagatg gacgacagct ccacgccacc taaagtcgca cggcccccag tgggatgggc
     5041 gaggcgcatc atcgtgcgta gccatgtcgt cttgccgcag cccgatcggc ccgccacgcc
     5101 aatcgtttct ccgtgccgga ttaagctcga gagaccattg agcgcgggtt tggccgctcc
     5161 gtcggcgtcg cgatattcga cgcaaagctg gtctgtgaca aacaccggct cacccacaac
     5221 cagccgcggt tcgtcggccg ctgacaactc gaacgatcga tcgaggggtt cagcaagcag
     5281 gccgagtagg tcgccgactc tcaggctgct ctcgtgcgct tcgtcaatga aacggtggat
     5341 ctcgttcagc ggggccatga cgttcaagta cagcatcgag aacgtgaaga tttccccaga
     5401 cttgagtccg ccgtggacga acagatagat ggcgaacgcc agcacaacca cgtgaaagaa
     5461 accctcgttg atcgccttac cggagccaaa gagcgacatc tcgaaatgat gccgcagctc
     5521 tttggctcgc cggtactcgg ctgcccgctc gacgcgctcg acctccaggc ggtcggtgtt
     5581 agccgcccgt atatagtcga tgccgccgag ctgctcgacg accgtgccgt ccatcgcctc
     5641 gcgactggcc agcaggtcca gtcgaatgcc cttctgagtg atgagctgcc agattgtcag
     5701 accgagcgac accggaatca ctccggccat cgcgagcgca atcctcggtt gcttcgccaa
     5761 agtcgtcgac aaggcaaagg tccccatgaa caaggcaggg acgaaatcga gaaacccgac
     5821 tcgcagaaac cgaaccagcc cgtccactcc ccgggtgata cggccgtaca gaccaccgac
     5881 ctggtcatga gccagaacgc ccagatcgac cttgagcaag tgcgacacaa tacgcacata
     5941 catgtctctg tcgatgcgtg tgcacgtgtc ttcgaccaga aatcgccgca agacattcat
     6001 gacttcacgc acgagatacg cgccgccaat aatcgcgaga taaacagcgg cgatccgcgt
     6061 caggtcggct accgactggc tggaatgctc gtaggggtta accgcgtcga tcaatctacc
     6121 cacgcacaat gcgatcgctg tgttggctcc gctggcgaga ctcatgattg caagagcgcc
     6181 tgccagcgac atcttgtgac gcccgggaat tagtcgccaa acttcgcttg cctggcgacc
     6241 aattgtcgcc agttcttgcc tgattccctt gaactcgcca ttcttccttc gcctcatcgt
     6301 cgccctcttt ccgtccacgc agtcaatgcc tattttgtgg aggcgctaat tcaatcggga
     6361 accgaaccac tcggagattt tcatttcgcg gtatctggac cagattccgt attaaatgct
     6421 aacctggtga gcaacttgcc cgagccagag tcctggtgga ggcatgggaa agctgtccat
     6481 tgcccagttt gccactttct ccacaacaaa gacgacaaaa tcatggacag atcgcctcgc
     6541 cagtcggtaa caactcgttt catgattctt ggcgcgagat ttgctgagtc cggcagctat
     6601 tcggagccgt caacgacact ttgtgatttt gacacgcttt gaattagatt cctttctcgc
     6661 tagcgcccta ttctgaacta cgcgggatca caccatagat gaaagaagat aaggattaat
     6721 tccagccaaa tccttgttca ataaactcga tgctcacttg gcattgattt tgctagcacg
     6781 tccagcacat ttcattgacg acgattcaat cccacgggga gggagcagct tcctaatgat
     6841 gaagatccat acttcgagta actcagtaac ggacgccaat gctgtctgga ggtcgattca
     6901 gcacgaatcc atcgatgcga atcgacgttc ccaatcgcga ttgtcgttac cggagctatt
     6961 cgcgtcatcc ggtcccgaag aaatcttggt tgtagaggaa cgggcccatc tccgaccggc
     7021 acaggtcgtg gagagtaagc tgacgcggct gctacaggag gcttcgtttc ccgtgagcca
     7081 cctccagacg agagggctat tgaagactcg ctaaaccgtc tgtctcgttt agcgccccca
     7141 tcacgacgcg cacttcatgg tttttgccat cgctggtcag tcgcaggccg ttacccggct
     7201 gacgcgcgtt atcgagccaa accgccgcga caccggactc aacgccggct gggttttgaa
     7261 ccttgatcaa gtaagttgtc gaccggagcc ggtaagtcat ctcgaagtgg gaccagtcgg
     7321 gcgggacaca aggattgaac gcgagacgat ctcctacgcg ctcaattccc aggatcgact
     7381 cgacaaacac tcgataaaac cagccggccg agccggtata ccaggtccag ccgccgcgcc
     7441 cgacatgggg cgcgcggctg tagacatctc cggcaaccac gtacggctca accttgtatc
     7501 gctccactcc attcggatcg tcagcatgga ggaccggatt gatgatactc agtaactggt
     7561 acgcgcgctg gccttgccgt tgacgggctg ccgcctccac gacccacgtg gcggcgtggg
     7621 tgtattgacc tccgttctct cggacgcccg gaagatatcc tttgatataa ccgggatcga
     7681 gtgcgtcgcc gtcaaacggc gggtcgaaca gcaatatcag gcgatcggcc tctcgtacaa
     7741 gaagatcatt gacggcttgc atggccaggt gctggcgctc tggactaccg ccgccggaaa
     7801 gcacggccca cgtttgtgtg attgagtcga tcttgcaggc actgttttga gaggaaccga
     7861 gcggcgttcc atcgtcaaaa taggcgcgac gataccattc accatcccag gcgctttcct
     7921 ctacggttag tcgtagctcg tcggcctgcc gtcgcaattc cgcggaacgt tcgtcatcac
     7981 cgcgctcgag ggcgattgcc gcaaatcgga tgcggcaaat gatcgaaaac catgtcagcc
     8041 aaacactttc gcccttgccc ctgctgccga ctcggttcat gccgtcgttc cagtcgccgt
     8101 ggcccatcaa ggggagacca tgcgcaccaa ggcgttgtgt gtactccagg gcgcggagac
     8161 aatgctcata cagcgggcgc gactccgccc cgggttttgg caggccatag tcgtcgtcct
     8221 ggccgggctg caaaggtgga ccattcaaat acgggatcgg ctcgtcgaga atcgcggaat
     8281 caccggtcac ggttacatag cgctgcgtaa cgaatacgag ccagagcggg tcgtcagcaa
     8341 ttctggtacg gacgccacgc cctttgggcg ggtgccacca gtgctggacg tcgccctcgg
     8401 ggaactggcg ggcagccgcg cgaaggatat gcgtccgcgc aagatccgga gcggcgtgga
     8461 aaagcgccat cacatcctgg agctggtcgc gaaaaacata ggcacctccc gattgataaa
     8521 acgcggaacg tccccaaaca cggcaggaaa gcacctgata aagcagccag cggttggtca
     8581 aaagatcgag cgccgggtcg ggggttcgca cctggacggt ctcgagcagc tcatcccacc
     8641 gcgccttgac gtcgctgagt gccgatgccg cccgcccctc ctcgcgatag cgtttgatca
     8701 aggcgcgtgc ggcgtccggc ccctcggcct cgccgagtag aaagacgagc tgggttttgg
     8761 ctccagcttc gaggctaaat gcgacctgga tcgcggcgca aggatcgagg gcttcgccgg
     8821 tcgctcccga caattccact cgtcccagcg cggcaggggc catcatcgat ccgtgccgcc
     8881 ccacgaattc ggcccggtcg gcggtgaagg atctgggccg gcggtcgacg tcggcaaatg
     8941 cgacacgtga gccgaagtcg gtacggaagg tattgcgcgc caagagcgct ccggtttccg
     9001 ggtcgacctc agtgacgaca tgcatcgcgg tggcatcgcg cgtcgagccc acgacccact
     9061 cggcgtagta agttgccgag agccgtcgcg gccgatcgct caaattctgg atccgcaaga
     9121 cgatcagctt gacgggatcg tcggccgcga ccagcagggt cagctcatgg aagaggccgt
     9181 aggcgttttg ttcaaaagtc gtatagcctt gtccatgacg aaccagggtc ggctggctcg
     9241 acggagcggg cagcggcgtc gggctccaga tatcaccgtt ttcctcatca cgtagataga
     9301 ctatctcgcc cggggggtcg gcgacggggt cattgctcca gggtgtcagc cgatttgcct
     9361 ggctgttttg agaccatgta tagcccgagc cgctctcgga aatcaggaaa ccgaatcccg
     9421 gattcgcgac gacgttgatc caaggtgcgg gagccagccg cgggtatagg acggcatggg
     9481 acaccggctg cccgttaccg cggctatgcg ctctgtcgtc cgatcttatc aacaggcagt
     9541 attcccgccc atcgggggtg aaaccgccga gcccattggg gaagcgcaga tctgacggca
     9601 gagtaaccgg ctcatcctgc cacctgggat gctcgtgtga catggtcaaa gggccgggga
     9661 gcggatgtcg tagctcggtt cggtcgagct gactggcaag cgagcccccg tcgccgacca
     9721 agactgcccg tgcggcggcc tgaatcagaa tctttccgtc ctcgggtaac tcggcggctt
     9781 tcagcaagaa gattcctccc ggttggccga ttcgatcgga gccgccgtct gtgcgaacaa
     9841 cactcatgag ctgtcggctt agttcttcct catggccagc gggctgatcg ttgagtaaga
     9901 ccaggtcgag ctcgagcccc ttgccgcgga gatacgtctg agcggcgagc agttcgcgag
     9961 cgaggtggat ctcatcggtt gcaccaaatc gggccaccac aatcggccga tcgcccgaga
    10021 ttccaaaggt ccagaggcct tcctggccca aagtattgct cgcaagtatc tggcggtcgg
    10081 ctcgcaacgc cgatcccgcg aagagcacat gcgcggcgag acgttgaaac agatgggcgg
    10141 actcgccgaa tcgatcgcta tgcctgtgct cgatctggct atgcgcccac gccaactcaa
    10201 acacacgcga cgcggcgctg gcctggcgat actgatcggc cagagccagt ccttcggcgc
    10261 gcgtactggc gacggcggtg gccagggcga tcgtggccga cccaccgggc ttgagccgaa
    10321 tccgttggcg taggcaaaag acagggtcaa gaaccggtcc gacggtgccg gaaaggacgg
    10381 aaccggagtc gagtgccgcg gggttggcca cggtgcgtcc gcggccgatg aagcggcacc
    10441 ggtcggtctc atattgcctc tctcccacgg tcgcactgcc cgctgcgttg acttctacgg
    10501 ccgccacatg gacggcgcag atcggcggct cgtctacgga tcgcggccgt cgccggcaga
    10561 gcagcgcgcc ggagctagcc acccattctg tctcgaggaa gagtttaccg aacgctggat
    10621 gggccaggtc gtcccgatgc ggcgccagga cgacctcgga gtaactggta aggtcgagct
    10681 cgcgagggcg ggaatcgtga ttggtcaacg tgactcggcg gacctcgacc ggttgttccg
    10741 gcgagacggt tacctcgatc aaagtttcga tctcgccgtc acggcggcgg atcgtgctct
    10801 tgtcggcagc gaaaatgact tcgtactcat cagcgcgctt gcagaccggt tgatatccgg
    10861 cggaccatac aagatcgcgc tgcaagtcgc gtatgtagca aaactggccc cacgtttcgc
    10921 acgtgggatc ttcgcgccag cgagtgacgt ccatgccccg acagatgctg tagcccgatc
    10981 cggcattggt gatcatcaca tggtattgcg tattggataa aaggttggtg cgcggcgcgg
    11041 gcgtggcagg cgatgtcagc cgccggctca tcggtgccag cgggcccact ttgtcggccg
    11101 cggagggtgt ggatgccgtc aatttggcat ccgtggtctc gatgatttcc ggatcgctcg
    11161 ggagccgttc ctgcaggagg agttcgagtg cacgcacgag cggctcagcg tgaaaccgct
    11221 tgggcatcac atcgttcagg agcgtattgg cgagcgccac caggctcatt ccctggtggt
    11281 gcgccatgta cgacctcatc accactcgcc gctcatcggc ggggattcgt ccgcgcgtgt
    11341 agtcgatcgc ttcgtacagg ccgaacggac catcggcccc ttcttgggcg agccggcgga
    11401 tgttgtccaa agcctcgtgc ggcacgagca gcgccgccaa gaccgtcgca tagggtgcga
    11461 caacctgttc cttttcaagg ccctgcttga gtcccaggcc gggtactccg aaagcctgat
    11521 actggtaatc gccatcgggg tactgagccg agaatgccga ctctgaaacg ccccaaggcg
    11581 tgcccagttg ccggccgtac tcgatctgcc gcgtcacggc cgtttgacac gcctcggcca
    11641 gaatggtgcc cggcaggctg cgcaacaaca gccgaggcat aagatactca aacattgtgc
    11701 cgccccacga aaggagcccg atcttgccgc ccgcgctgat gaaatgtcgg ccgagctgga
    11761 accagtggcg gcggggagcc tcgcctcgcg cgacggcgag atagctcgtc aaacacgctt
    11821 ccgacgccag taggtcgtag caagcgttgt cgagcttccc ttgcgccagg ttgaatccga
    11881 tcgcaaacag gtgtcgatcg ggcttgtaca gcggccggaa atccatcgtc gccgccaggt
    11941 cctctgcgcg gtcggccaga tggcgcagcc ggctcagcca ctcttttgtc cgcgattcgg
    12001 caacggccgc ggccacctca gcgcgccacg acctgacctg ctcgaccagg cgaccggtcc
    12061 agccccccgc ttcccacttc gttgcggccg cggcggagtc ctttgcgtct gtgcgggcgg
    12121 ccagatcgac cgccgcgcct tcgacctcct cgagccaact gtcccattca gcgagatcgc
    12181 ccggtgtcac gtctagtaag gctttcaagc gccgacaatc ctcgcccgtt tcttcagcga
    12241 ccaagaagac agtgtcggcg agcccctcta tcaccgtggg cccgaccacg ggcttttcgc
    12301 acttctccaa aagtccctgc ttgagcgcga tcagacagcc gagcaagttg ccgctgtcca
    12361 ctgtcgaaat gtagcgagga gagagaggct gcatcgtgcg ggtgtcatac cagttgtaga
    12421 agtgacccca atgcttctcg agccggtcga atgtatcgaa agttcgctcg agccgatcga
    12481 tcaaggtaga caggcctatg tatcccaggt cgtgggcggc cagggtcgag agcaacagca
    12541 gtccggtatt ggtcggcgac gtgcggtggg cgatgcggcc gtcggggact tcctggaagt
    12601 tgtcgggcgg gagccaatgg tcatcttcgc cgacgaagat ctcaaaaaat tgccaggtct
    12661 ttcgcgcgat gcgacgcagc gcgcggcggt cgtcgtcggc cagtgaaatc tgggcgatcg
    12721 gccggggctg gctgactcga aaagcgaccg ccggagataa gagccatgcg aaaaggaaag
    12781 gacccgcagc tggcagggcc gccggtcgca ccaggagcac gacagtgccc atagcgatcg
    12841 ccaatgccgg tgccacccac attcccgtga cgaattgtac gagccccccc ttgagccgca
    12901 gctctgtcga cgccgcggtt tcccactcga gaagcttgcg tcgcgtccag agcaggcgaa
    12961 cgagagttcg aatgaccgcg tcgagcagaa gcactccgcg ataggccaga aatgtcacgt
    13021 cgagcaggac ttggccgatc acggcgggcg ttccgaacag cccggtgtaa agtccggcga
    13081 gcgaccggcc gcggacagac tcgaccgcac tgccaattat cgattgaata agaggtagcc
    13141 cgagcgttgc cagggcaaca cctgaccaga gccagggaga gccaggcaag accgtccagc
    13201 ccagcaataa caacaccaac agtgcggggg gaacgaggct acggcgcaaa ttgtcagcga
    13261 gcttccagcg ctcgaggagc ggcaacgggt tgggccgcca tcccttcggc gtcggcaccc
    13321 gtcgacccag ccaggggagc agttgccagt cgccgcggac ccagcgatgc tcacggcggg
    13381 catacgcgtg gtacagcgcc gggaagtcgt cgaacaactc ggtgtcgctg atcaagccgc
    13441 agcgggcaaa attcccctcg atcaaatcgt ggctgaggat gtgattctca ggaaacctgg
    13501 aaccggtggc cgcctcgaag gcgtcgacgt catagatccc cttgccggtg aaactgccga
    13561 ccccgaacaa gtccatgtag gcgtcggagg cggccgtcga atacggatcg atcccccccg
    13621 cggtcgccag gagagcggca aaccgtgagt gcgtggcggc cgccaggtga aagctgatcc
    13681 tcggctgcaa tacgccgtaa cctgtggtca cacgctcctg gttcgggtct aaccgtggcc
    13741 gattgagggg atgggccagc gtccccacga gtcgcccggc agtgtcgcgc ggcatctgcg
    13801 tgtcggcgtc gagcgtgatg acgaagccga ttcggggcaa ggccgcagga tcggcactca
    13861 ggaccgcata gctcgtatcg cgggcgcccc tcagcagccg attgaactcc agcaatttac
    13921 cgcgcttgcg ttcccagccc atccagcaac cctgcgcctc gttccagagc cggcggcggt
    13981 gaaacaggaa gaataggttc gtgccttccg gggcatagcg atcgttcaag gcccgcaccc
    14041 gctcgagagc gtcgacgatg atcccctcgt cctgaggcat cgtctctcga ggagcgtcgg
    14101 taaagtccgt gagcaaagcg aatcgaaggc ttgggtccgg gttggccagg tagtggagct
    14161 ccagccgctc gaggagaacg gccccgcttg ccggccgtgc cagcagtgat ggaatgacga
    14221 cgaacgtagt gtgttcggtc gggattccat gcttcacgtc aagcttgggc aaaacgcgcg
    14281 gcggcacaaa cagggtaaat agctggttga ccaaacccac caccagctcg gagagcggca
    14341 acaaggccgc cgccgcgatc gcgaggatcc accaaaaatt ggtgaggctc cccccgagcg
    14401 cgaaaataac gaccagggcc aacagtgcgg caagcccgat gacgatcgaa ccgaagtaga
    14461 ccgattgcgg ccgccccagc acgaggtcga gcaagcgctc gcgccaggtg gggcgaaagc
    14521 ggaacgcgct catgagctct tgctggccgc ggtcgatcaa atagaagccg acgtggtcac
    14581 gcggctcgcc cgagccacgg ccctgcaccg ccagctcgac cgctttccgc gccacttcca
    14641 tctcgtccgc gtccgattgc cgggcaatga tctcgactac ccggcgatac cggtcgctgg
    14701 tggcaaactc ctggcgcggg taaatgcctg cgggatcatc tcgcaggatc gcttcgacgg
    14761 gactgctgtt ctcgaagaat gcattccagt cgatggccga gagtaggcgc ag
//
LOCUS       DATAQL010000984         5012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_711045, whole genome shotgun sequence.
ACCESSION   DATAQL010000984 DATAQL010000000
VERSION     DATAQL010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711045"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VHS97_16995"
     CDS             complement(<1..967)
                     /locus_tag="VHS97_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449955.1"
                     /translation="MQSGIVVLFALTSFVGAFLLFAVQPMIGKMALPLFGGTPAVWNT
                     SLVFFQGTLLCGYLFSYGLGRTGWSLRGRAGAVYLLGVVASLALGYFMQPIVLEPNSD
                     RRSSSAGDPALVLLGVLLRSAALPLVLVSATAPLVQRSFALTGHRRADDPYFLYAASN
                     AGSLLALLIYPFVIEPNLSLTAQSQLWKTGFLILVALLVACVLLARWLNRSRPVGNSP
                     RVFGLHGAEGRFALAIWRRRLALVFIPSSWLLGVTAYLTTDMASVPLAWIIPLALYLL
                     SFILAFARSAVAFVRLATRSLPFLIAPLVLVMSAGFVHFYWIPLHL"
     gene            1160..3088
                     /locus_tag="VHS97_17000"
     CDS             1160..3088
                     /locus_tag="VHS97_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3449956.1"
                     /translation="MSSLHRRIASRLRFRDYAKNYRDGSKPALSSSAEPPPSKHRSLA
                     RLYRELYGLLVGHRFAIALALLGLTVASLLKLIPPLATKVVIDNVIEAKPLPAWIVAW
                     SPIAIPASPKARLFVVVALVFVVSVVGKTVALTSRWRATKATKKVQVAIRRKVYEHAM
                     RLPLHRVYQLKSGGASSLLREDAGGVGELIFSMIYNPWHALIQFLGGLVALAFIEWRL
                     LCCALVLVPGVYYSDLIWNRRIRPLFRDVRKKRQEIDSQTTEVFGGMRVVRAFGRQRS
                     ESARFMGENHFMVRLELYVWWLSRLIELLWEFVLPMASVALLLYGGMQVIDGRLSLGE
                     LMMFLVYLAMLLEPMAVLASSVTQFQNNLAGFDRVLDLLAEPREMVDCPGTRSVSKSA
                     VAGQITLRNVGFVYPGTSRPVLRDINLEIEPGETIALVGRSGSGKTTLCNLIARFYDP
                     TTGMISLDGVNLREIEVENYRRLLGIVEQDVFLFDGTIAENIGYGDRWATRARTEHAA
                     RVANAAEFIDRLPDGYNTLIGERGVRLSGGQRQRLAIARAVLADPKIFILDEATSNLD
                     SESETLIQQSLGDLLHGRTSFVIAHRLSTIRHADRILVLEDGAIAEAGSHGELMAGSG
                     LYRDMVELQRIEQGDSVD"
     gene            complement(3175..4122)
                     /locus_tag="VHS97_17005"
                     /pseudo
     CDS             complement(3175..4122)
                     /locus_tag="VHS97_17005"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
BASE COUNT         1089 a         1499 c         1485 g          939 t
ORIGIN      
        1 tcaagtgcag gggaatccag taaaagtgaa caaaaccggc gctcatgacc aacaccagcg
       61 gtgcgatcaa aaaagggagc gacctggtgg caagccggac gaatgccaca gccgaccgag
      121 cgaaggccag aatgaagctc agcaagtaga gcgcaagtgg aattatccac gccaacggga
      181 ccgacgccat atcggtcgtc aggtaggccg tcacacccaa gagccagctc gatggaatga
      241 aaaccaatgc gagccgtcgg cgccagatgg ctaacgcgaa gcgtccctcc gcgccgtgca
      301 agccgaacac acgaggcgaa ttcccgacgg gtcgagatcg attcaaccac cgggccaaga
      361 gaacacaagc cacgagaaga gccaccagaa tgagaaagcc cgtcttccag agctggcttt
      421 gagcggtcaa actcaggttg ggctcgatca cgaacgggta gatcaaaagc gccagcagac
      481 tccccgcatt gctcgccgca tagagaaaat aaggatcgtc tgcgcgacga tgaccggtca
      541 gagcaaaaga gcgctgcacc aaaggcgcag tcgccgatac cagcaccagc ggtaacgcgg
      601 ccgagcgcaa caaaactccg agcaagacca aggccggatc gccggccgac gaggatcttc
      661 gatcgctgtt gggttcgagc acgatcggct gcatgaagta tccgagcgcc agtgaggcaa
      721 cgacccccaa gaggtacaca gcgcccgcac gtccgcgcaa gctccagccc gtgcggccca
      781 gcccgtacga gaagagatac ccgcacaaga gcgtcccctg aaaaaagacg aggctcgtat
      841 tccagacagc gggagtgccc ccaaagagcg gcaacgccat tttgccgatc atcggctgca
      901 ccgcgaacag gagaaacgca cccacgaacg aggtcagggc aaagaggact acgattccgg
      961 attgcatgaa ttcccgactc tcgttttgtt gcgcgagcgc aagcattcac ctcgcgtttg
     1021 gcataaacca acgagggcac gcgccaggcc gtttgtgtgg ctggggaggc gaggttagag
     1081 cgaacctact acaataacaa gtccgagcgc ggcgtaggat gcccgcaagt tccttcggcc
     1141 tatctcaaga ggatcatcct tgtcatcatt gcaccgacga attgccagcc ggctccgatt
     1201 ccgtgattac gccaagaatt accgggacgg ttccaagccg gctttgtcga gctcggccga
     1261 gccgccccct tccaagcacc gctccctggc acgactctat cgcgaacttt acggcctttt
     1321 ggtgggccat cgtttcgcaa tcgccctggc gttactgggg ctcacggtgg cgtcgctgct
     1381 caagctgatt ccccccttgg cgaccaaggt ggtgattgac aatgtcatcg aggcaaaacc
     1441 gttgccggca tggatcgtgg cgtggagccc gatcgcgatc ccggcatcac cgaaggcgcg
     1501 gttgttcgtc gtggtggctc ttgtattcgt cgtatccgtg gtcggcaaga ccgtggccct
     1561 caccagccgc tggcgcgcca ccaaggcgac caagaaagtg caggttgcca tccgccgcaa
     1621 ggtctacgaa cacgcgatgc gcctgccact gcaccgggta taccagctca aatcgggcgg
     1681 ggcctcgagc ctcttgcgcg aggacgccgg cggcgtgggc gaactcatct tcagcatgat
     1741 ttataacccg tggcacgcgc tcattcagtt tctggggggg ctcgttgccc tggcattcat
     1801 tgagtggcga ctcttgtgct gcgcgctcgt gctggtcccg ggcgtgtact actccgacct
     1861 gatctggaac cgccgcatcc gccccttatt ccgcgacgtt cgcaagaaac gccaggagat
     1921 cgacagccag acaaccgagg tgttcggcgg catgcgcgtg gtgcgcgcct tcggccggca
     1981 acgaagcgag tccgcgcggt tcatgggcga aaaccacttc atggtccggc tcgagctata
     2041 tgtctggtgg ctgtcgcgat tgatcgagct tttgtgggaa ttcgtcttgc ccatggcctc
     2101 cgtcgctcta ttgctttacg gcggcatgca ggtcatcgac ggccggctct cgctgggtga
     2161 actgatgatg tttctggtct acctggcgat gctcttagaa ccgatggccg tgctggcctc
     2221 gagcgttacc cagtttcaga acaacctcgc aggcttcgac cgggtgctcg acctcttggc
     2281 cgagccgcgc gaaatggtcg attgccccgg aacccggtcc gtgtccaaaa gtgccgtcgc
     2341 cgggcagatc acgctgcgca acgtcggctt tgtctatccg ggaacgagcc ggccagtgct
     2401 gcgcgacatc aacctcgaga tcgagcccgg tgaaaccatc gccttggtcg gccgcagcgg
     2461 atcgggaaag acaactctct gcaacctgat cgcccgcttt tacgacccga ctacgggcat
     2521 gatctcgctt gatggggtga acctgcgcga aatcgaggtc gagaattatc gccggctcct
     2581 gggaatcgtc gagcaagatg tcttcctctt cgacggcacg atcgccgaaa acatcggcta
     2641 tggcgaccgt tgggccacgc gcgcccgtac cgagcatgct gcccgggtgg ccaatgcggc
     2701 ggagtttatc gaccggctcc ctgacggcta caatactttg atcggcgagc gcggcgttcg
     2761 cttgagcggc ggccagcgtc agcggttggc cattgcgcgg gccgtgctcg ccgaccccaa
     2821 aatattcatc ctcgacgagg caaccagcaa cctcgacagc gaaagcgaga ccttgattca
     2881 gcagagtttg ggcgacctgc ttcacggtcg gacttcgttt gtgattgccc accgcctgag
     2941 caccattcgc catgccgacc ggatccttgt actcgaagac ggcgcgatcg ccgaggccgg
     3001 cagccacggc gagctcatgg ccggcagcgg gctttatcgc gacatggtcg agctgcaacg
     3061 gatcgaacag ggcgactcgg tcgactgacc ccccgacatg gcggtaaagg aaacagccga
     3121 gcgcagaccg cgcccggccg gtttccgaca aaacacaatc tggatgccag ggcttcaata
     3181 ttgatcagag ctgatcacct cgccgccggc acgcgtgccg agcccccacc acggctgcgg
     3241 ggcgatcgaa ttcttgatga aatgcaccga cccgtcggca aagcagagat tcacgccgcc
     3301 agggtgcaag ctattcgccg acgccgaacc gtacggaccc acatagcccg tggtgccgtc
     3361 cgtgaaggcc gagtccagcg agttcgtgca cggggccgtg ttgggtgccg tccagtgcat
     3421 gtagctcgtt agactgacgt acgctggatg gccggcaaac atctgcacgc cgacggccgc
     3481 cggatacgtg gccgccgcag tgctcgggat cgaattgcat ccctgcgcaa acaattgtgc
     3541 agggtttgcc gtgatcgcgt tggtggcgtt caccgagttc gggggcgtgg caatcgtgcc
     3601 cgtgaagatg gctcgcagac cggaaatgcc ctgagaaaaa accgtcgtgg tcgtgccgta
     3661 cacattagca gacaagagcc gctcactgaa gagccccgta ttcgatgtac catccgtgat
     3721 cgactgaaga ccgataacgg gcaacctggc tgcgttggca ttcattccac tttcaacatc
     3781 gaaccccggg acaatcgtgc cggtgtacgg ttggaatgcc gccggtccac cgtaattgcc
     3841 gacgtaattg gtaacagcgt agacggatgt gttcgtcccc gtattgacgc tgaagagctg
     3901 tagattgccg gattccgacg ggcagaggag ggagctcacc ttggcggagg ccgccgttac
     3961 ctgactgaaa tccatcggcc ccagagaaaa gttcacagcg ttgaaaatct gcgcctgctc
     4021 caactgtggg tagagagcgt agtaccaggt aaatccccac gaaaagccag cggtattggt
     4081 cggattggta ttggctgaca gcgtcggcca gcttgtctgt ggcgggaata cgttgttgga
     4141 ggaacagcgg cgagcattcc cgtattcatc tcgtcacgga ccgatgccca cacggcccga
     4201 tggcgattcg gaatgccata actcgaattg tttaattaca tgagtgtatt tagccaatgg
     4261 ctagccgacc gatgcgacga tgcgcttcat tgctcgtttg acgtgtcatt cgtcgaacac
     4321 acccggaaaa cggagtgcca aacatcggcg cggcgagcct tgccgatgcg agaccgatcg
     4381 cggcccgagg acttaccaca aaaacctcgg ctctcagcgg cagcgtttgg gctttgtggg
     4441 tgggatatac atcgatcgag gacacatcca gccgcgaaac ctttagaccc agcaactggc
     4501 gtccagaccg tgacgtatct cctgatcttt taatatatcg gtgggctaaa gcaatttcta
     4561 cctggaagtt tcgcgcaacg agcgaattaa gaggttcctc gtgatcggga aacgatccgc
     4621 cccgcctatt cgaatgctct aacatcaatc gctgcaagga ttggcagcta taaatgccag
     4681 acggtttcaa tcggtcagat ccggcgtcaa agaattcttt cttaagaaaa tctaagaaac
     4741 cgagcgcgtt gacacagttg tatcctcacg ccatacccat cttcgagcgg cgtcagggcg
     4801 cgagattgcc gaggatcgca caagttggat ccgatccttt gccatcggct tcagcgccgc
     4861 tccgtcaaaa tcgccttgac cccgtgcgcc ggccgtagag tgatcgtggg caaagggacg
     4921 attttggcgt cgggcgcgag cttgagccgg tacttgcggg caatcgtcgc caggatcagc
     4981 gtcgcctcca tcagagcgaa gttgttgcca at
//
LOCUS       DATAQL010000985         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_404729, whole genome shotgun sequence.
ACCESSION   DATAQL010000985 DATAQL010000000
VERSION     DATAQL010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404729"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1119
                     /locus_tag="VHS97_17010"
     CDS             109..1119
                     /locus_tag="VHS97_17010"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3449957.1"
                     /translation="MPLIVERPRQGSLTKHEFARDAKRRPKWIFQPQQVVCLEERALL
                     STFAMTSAAAASLSVSLHAGRNQPQLAAPLTEIHNVPFPTVDGQSELLDVYMPRTPAP
                     AAGRPVIIAIHGGGWRRLDKAGYGSRVAAAFVRDGYVVVAPNYALSAPGKPTWPVNFE
                     DVQAAVRWVRTSSGALGINPNEIVAMGESAGANLAALLGTYSTQSSGDGVSSAVDAVV
                     ASSTPTDLTSVYSESPLAGLATAQFLGGSPAEVPANFIAASPIDHVSPGDPPMLLIHG
                     RQDPLIPVSQSEQMRAALTAAGVRNELILVNGEHDLNFPPNYSNLIRDVLQFLDTTWK
                     AP"
     gene            1588..2418
                     /locus_tag="VHS97_17015"
     CDS             1588..2418
                     /locus_tag="VHS97_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3449958.1"
                     /translation="MDARFHAAQAALAAGDVERLEALLKSDPGLAIAQSTSDHPTILQ
                     CLVLTMPPVDTLEKLIGLLADHGAELTDPLIAACGPGNVRAIAKLLDLGANIEGNGRW
                     SPLDEAAYFGQEPCLSLLVERGAPVANIRTAAALGRLDLIKLWLDATGPLTTAACEIA
                     WPFSMPIPEELRQDRRQILGNAFIYAAMWGRLEVAEYLLNCGAQVNFIPVGFDYSGTP
                     LHYAAFEGRREMVDWLLRHGADPSIRDTKIGKLPEDWAEHSGHKDLAEQLQLVRRRAE
                     "
     gene            complement(2456..3133)
                     /locus_tag="VHS97_17020"
     CDS             complement(2456..3133)
                     /locus_tag="VHS97_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449959.1"
                     /translation="MLWIRLRFLTVVIVAVGIGSGGAGVYVLGSQDPAPKDGQLAPKR
                     LTTTTAETPQPKISNQETQEPASDSPRARLRGQRLATRKAKAFYEIAKATRELAEIGD
                     EEYEDVNFPNELAMVEGEIKLSDSDLSRSEDRVDWAKRMFDKGYVSRATKVSEELTLK
                     KARFALEQAQSKKKVLVDYTKGKTIKELKSEVEKARSNELAKQATWELERLKELELER
                     LLSTRPN"
     gene            3153..>3333
                     /locus_tag="VHS97_17025"
     CDS             3153..>3333
                     /locus_tag="VHS97_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449960.1"
                     /translation="MSERTRAETSNPVNTRADCCIAALTALRETFFGIAATAVRLKDV
                     TTVPATAGPNPRRTKW"
BASE COUNT          663 a         1040 c          938 g          692 t
ORIGIN      
        1 atgcgaaatt gcaatcggac gctttctcga cgtggcgata gctctgcctc accgttgcaa
       61 tcagtacaat tccgaaaagt gaaacgttca gaaatgagcg tccatcccat gccactcatc
      121 gtcgaacgac ccaggcaggg ctctctgacc aagcatgaat tcgcaaggga cgcgaaacgg
      181 cggcccaaat ggatttttca gccgcaacag gttgtctgcc tggaagaacg agcgttgctc
      241 tcaacattcg cgatgacgtc cgcagcagcg gcctctttat cggtaagctt gcatgccggg
      301 cgcaatcaac cgcaattggc tgcgccgctg accgagattc acaatgtgcc ctttccaaca
      361 gtggatggcc aatccgagct gctcgacgtc tacatgccgc gcacacccgc acccgccgcc
      421 ggacgcccgg tcatcattgc cattcatgga gggggctgga ggcgattaga caaggcgggg
      481 tacgggtcgc gagtcgccgc cgccttcgtg cgggacggtt acgtggtggt cgcgcccaac
      541 tacgccctat ccgcgcccgg caaaccgacc tggccggtga attttgagga tgtgcaggcc
      601 gcagtgcgct gggtgcgcac cagttcgggc gcgctgggga tcaacccgaa cgagatcgtt
      661 gccatgggag aatcggctgg ggccaatctg gccgctttgc tcggaacgta ttcgactcaa
      721 agttctggcg acggagtgtc ttcggccgtc gacgccgttg tcgcgtcgtc gacgccgacc
      781 gatctgacga gcgtctacag tgaaagtcct cttgcgggtc ttgcgaccgc ccagttcctg
      841 gggggctctc ccgcggaagt tcccgccaac ttcatcgcgg catcaccgat cgatcatgtt
      901 tcgcctggcg acccgccgat gcttctaatc catggccggc aagacccact gatccccgtc
      961 agccagtcag agcaaatgcg ggcggcgctc acggcggccg gcgtgcgcaa cgagctaata
     1021 ctggtcaacg gggaacacga cctcaacttc ccaccgaatt attccaacct gatacgcgat
     1081 gtgcttcaat tcctcgacac cacctggaaa gctccgtaag ccatatcatt ggtgtagaac
     1141 cgtcgtcgtt ggctgtcctc cccgcccgaa ttccttgccg ggcggaaatt cctggggcgc
     1201 tctttccagc aggttcccgc cgcctacatc gcggcatcgc cgatcgacca tgtttcgcca
     1261 agtgatccgc ccatgctctt gatacagggt cgactagagg cggcacgtag gtcgggcttt
     1321 ccagcccgac gtcggcctgg gaaggctagg atgcggacgt cgacaagaaa ttgaaagaat
     1381 tgatgaaatc ggaccccgac gttgaaaggg tcgtactgag cgctgttaag caaaaaaccc
     1441 tcgaaaagga aatgttcgag ccgttggcgg aagtcatcaa gaccgctcgt tcggaaatcg
     1501 cgcccgatcc aggattacga ttcaagtccc attgattaca atgccatcga tgattccatc
     1561 ggaccgaacg aatcgaagga gctggctatg gatgcgcggt ttcatgcggc acaggcagct
     1621 ctcgcggccg gcgacgtcga gcgtttggaa gcgctgctga agtcggatcc tggattggca
     1681 atcgcacaat ccacatccga ccatccgacg atcttgcagt gcctcgttct caccatgccg
     1741 ccggtcgata ctctcgaaaa gcttatcggt ctcttggccg accacggcgc cgaattgacc
     1801 gatccgctga tcgcggcgtg cggacccggc aatgtccgcg cgatcgcgaa attgctagac
     1861 ctcggagcga atatcgaagg gaacggccgc tggtcgccgt tagacgaggc tgcatatttt
     1921 ggacaggagc cctgcctgtc gttgctcgtc gagcgcggag cccccgtcgc caatattcgc
     1981 acagccgcag ctctgggacg cctcgacctg atcaaactct ggctcgacgc gaccggcccg
     2041 ctcacgaccg cagcctgcga gattgcctgg cccttcagca tgccgattcc ggaagaactt
     2101 cgccaagatc gccggcaaat ccttggaaat gccttcatct atgcggcgat gtggggccgt
     2161 ctcgaagtcg cggagtatct tctgaattgc ggcgcccaag tgaacttcat cccggtggga
     2221 tttgattatt caggcacgcc acttcactac gccgccttcg aaggtcggcg ggaaatggtc
     2281 gattggttgc tgcgccacgg tgctgatccc tcgatccgag ataccaagat cggcaagctc
     2341 cccgaggatt gggccgaaca cagcgggcat aaagatcttg cggaacaact ccagcttgtt
     2401 cgccgccgcg cggagtgact tgatcgcttc aaaatgccga acatgcttcg cacgttcagt
     2461 ttggcctcgt gctgaggaga cgctccagtt cgagttcttt gagcctctcc aactcccagg
     2521 tcgcctgctt ggccagttcg ttggaccggg ccttttcgac ctcgctcttc agctccttga
     2581 tcgtcttgcc ctttgtgtaa tccaccaaga ctttcttctt gctctgtgct tgctctaacg
     2641 cgaatcgagc cttcttgagc gtcagctctt ccgacacctt cgtcgcccgc gagacatagc
     2701 ccttatcgaa catccgcttg gcccaatcga cccgatcttc agaacgcgac aggtcagagt
     2761 cggagagctt gatttcaccc tcgaccatcg ccagctcgtt gggaaagttc acatcttcat
     2821 attcctcatc gccgatttcg gcgagctcgc gggtcgcctt ggcgatctca tagaaggctt
     2881 tggcttttcg tgtggccagt cgctgcccac gcagccttgc tcggggtgag tcggaggccg
     2941 gctcttgcgt ctcctgattc gagatctttg gctgcggggt ctccgcagtc gtggtcgtta
     3001 gccgctttgg ggccagctgt ccgtccttcg gcgccgggtc ctgagagcca agtacgtaga
     3061 cgccggctcc ccctgaccca atcccgaccg caacgataac aaccgtaaga aaacgaagtc
     3121 tgatccacaa catggcttga agaactccgt gagtgagcga aagaacgcgc gccgaaacct
     3181 caaaccccgt caacacacga gccgactgct gcattgccgc attgacggcg ctccgcgaaa
     3241 ccttctttgg catcgccgcg acggcggtcc gcctcaagga cgtgacaacg gtcccggcga
     3301 cggccggtcc gaatccgcga cgcaccaagt ggt
//
LOCUS       DATAQL010000986         3696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5291586, whole genome shotgun sequence.
ACCESSION   DATAQL010000986 DATAQL010000000
VERSION     DATAQL010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5291586"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VHS97_17030"
     CDS             complement(<1..814)
                     /locus_tag="VHS97_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449961.1"
                     /translation="MVSSRNRWLRSLLAGLGILAAPVGASTVWAQPPIDPFRPFVSQY
                     DAYRYPIGPATPDGGQSAPFALSGVRGANQYQNYLNELQGAGGAGVEHNGIGMPYYRS
                     AVDPRFDRDGSRQYQPNRKTDETFERTQALITEKYFAYFTEKDPKKRAELLRNFNRAR
                     SQVTRALSARGAKSERLLDAVSGNDVDSRPSVATGRRADTLRKPAESKPRAPSAGAPP
                     LRPNTDRARDDLSPSIPPAPPLFPGLGTRGSRTRRSPSDVLNRSLRLNMDDDA"
     gene            1285..2736
                     /locus_tag="VHS97_17035"
     CDS             1285..2736
                     /locus_tag="VHS97_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449962.1"
                     /translation="MPSVSWSRLGIVGGLMMLGGTLIMLAVLGQVQGGEPSQDLAALV
                     AQLGAARYADREAAAQAIERIGRPALAALRSARDARDLEVRTRASSLAQKIESLMLTQ
                     ATRVQLDFDRTPLPEVATALSRQAGFKIALYPSNLPRWRQQRVNWRAARPMDFWKAVD
                     QLCDLAGLQYNPSMQGYVGQGEPSFSLTEGTTRVLTPVSDHGPFRVSLLSVDYQRHVG
                     YAPSGHAANVPPPPRPAALEPDLRDQPLQQRLNPVTSVQFSAQLLVAAEPRLALSNVR
                     QPQLIEAVDDLGNSLVPVAAPDPFVSRHSGYFGMATGPVVQLQAPLQRPDNAGERIKK
                     LRGLIALNVSSRRPDPLVVPLNSAAGKTFENQDHRLTVHDIHPSPNSHHMLVELSIKA
                     VDLDQSSDHGEIDAFSDGLQRADPQHLQIEVIDAHGQLITWFQSGADAETGRFTLTLT
                     NLPQTSQPKELRYYTLTRASVTVPFEFTDIPMP"
     gene            complement(2899..3546)
                     /locus_tag="VHS97_17040"
     CDS             complement(2899..3546)
                     /locus_tag="VHS97_17040"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HEX3449963.1"
                     /translation="MFPIVVAICTLLATGLVHGYRTNRWGLSSELELARFRVEGLPMT
                     VGNWKGTPATLDGREILAAGLDGFVLRTYKNEATGKSLTLLLVCGRPGPVSVHTPEIC
                     YPGAGYEIVQAHPVKIQVGAESGGDFLMADFERRETIPADRLRIYWSWNASGTWSVPE
                     SPRIVFAPHPVVYKLYLISAVTKYREQSTEDGMADFLGQLLPELRTVLFPGSESR"
BASE COUNT          743 a         1091 c         1128 g          734 t
ORIGIN      
        1 ttgcatcgtc atccatgttc aatcgaagcg agcgattgag aacatcagaa ggactgcgcc
       61 gggttcgact tcctcgggtc cctagcccag ggaagagggg cggagccggc ggaatcgacg
      121 gcgagagatc atcccgggct cggtccgtat tgggcctcaa gggcggcgcg cccgcggaag
      181 gagcacgcgg tttggattcg gctggcttgc gtaatgtgtc ggctcggcgc ccggtcgcga
      241 ccgagggtcg cgagtcgacg tcattaccgg acaccgcgtc gagcaatcgc tcggatttag
      301 ccccgcgcgc cgacagagcg cgggtgacct gggaacgcgc gcgattgaag ttcctcagca
      361 gctcggcccg tttcttcgga tctttttcgg taaagtatgc aaagtacttt tccgtgatca
      421 gtgcctgtgt gcgctcgaac gtctcgtccg tttttctgtt tggctggtac tgtcggctgc
      481 cgtcccgatc aaaacgggga tcgacagccg atcgatagta cggcattccg atgccattat
      541 gctcgactcc cgcgccccca gctccctgca gctcgttcag ataattctga tactgattag
      601 cgcctcgtac gcccgaaagg gcaaacgggg cgctctggcc gccgtccgga gttgccggtc
      661 cgattggata cctgtaggca tcatattgac tgacgaaagg tcgaaatgga tcgatcggtg
      721 gttgggccca gaccgtcgac gcaccgaccg gcgccgccaa aatacccagc cctgcgagca
      781 aggatcggag ccagcggttg cgtgacgaga ccatgtcttg ttctcccaga atgacgattt
      841 gtccggttcg cctacagaat cctaggcgag gggggtgcac gatccgtgcc aacgatcgac
      901 gtgcaccgcg ctagtatgga ttgtatcaat acccatcgtc ttccgccatc tggtagatgt
      961 acaagtcaag tgggaaatcc accagtagtt ttatggtgag acagaagatg acggttgcgc
     1021 cggcgataca ccagggtgat cgcggataag cgacgcagcg attgattgcg gacgaagaat
     1081 agtgtttctt ggcgttggct gatcttggta agtaaggggg tggatgcgcg tcgccacagt
     1141 cggctgtgcg aagccgtttg ctggtcgaac gagaacaacg atgctctctt ttgtcaaagg
     1201 aaagagccca cgcgagtgga aacgcgcagt ggatcggcta tcatggacga tatgatcttg
     1261 tcggttggga atcgagtggc tttgatgccg tccgtttcgt ggtcgcgact gggtattgtg
     1321 ggagggctga tgatgctcgg tggcacgctc atcatgttgg cggtgctggg tcaggtacaa
     1381 gggggcgaac cgagccaaga cttagcggcg ctcgtcgcgc agctcggcgc ggctcgctac
     1441 gccgaccgag aggcagcggc acaagccatc gagcggatcg gccggcccgc actcgcggct
     1501 ttgcgaagcg cgcgcgacgc tcgcgatctg gaggtcagaa ccagggcctc gagccttgct
     1561 caaaagatcg aatccctgat gcttacgcag gcgacacgcg tccagctcga tttcgatagg
     1621 accccgctgc cggaagttgc cacggcgctc agccggcagg ctggattcaa aatcgcgctc
     1681 tacccgagca atctcccccg gtggaggcaa cagcgggtga actggcgcgc ggctagaccg
     1741 atggatttct ggaaagccgt cgaccagctt tgtgatctcg ccggactgca gtataatcca
     1801 agcatgcagg gttatgtcgg ccagggcgag cccagctttt cgttgacgga agggacgacg
     1861 cgagtcttga cgcccgtttc cgaccacggg ccattccgcg tgagcttgct cagcgtcgac
     1921 tatcaacgtc acgtcggcta cgcccccagc gggcacgctg caaatgttcc gcccccaccg
     1981 cggccggccg cattggagcc cgatcttcgc gatcagccgc tgcagcaacg gctcaatccc
     2041 gtgaccagcg ttcagttttc agcgcaactg cttgtcgccg ccgaaccgcg gctcgccttg
     2101 agtaatgtcc gccagccgca actcatcgag gccgtcgacg acctgggcaa ttcactcgtc
     2161 ccggtggcgg ccccagaccc tttcgtcagc cggcattcgg ggtatttcgg catggcgacc
     2221 gggccggtcg ttcagctcca ggctccgctg caacggcccg ataacgcggg tgagcgtatc
     2281 aaaaagttgc gcggactcat cgcgcttaat gtgtcgtcgc gtcggcccga tccgctggtc
     2341 gttccgctga atagcgctgc gggcaagaca ttcgagaatc aagaccaccg cctgacggtg
     2401 catgatattc acccaagccc caacagccac cacatgctgg tcgagctgtc gatcaaggca
     2461 gtcgacctcg accaatcgtc ggatcacggc gagatcgacg ccttcagcga cggcctgcaa
     2521 cgtgcggatc cccaacattt gcaaatcgag gtcatcgacg cccatggcca gttgatcacg
     2581 tggtttcaat cgggggcgga tgctgagacc ggccggttca cgctgaccct gaccaacctg
     2641 ccgcagacgt ctcaacccaa ggaactgcgc tactatacac tcactcgcgc gtccgtgacg
     2701 gttccgttcg agtttactga tatcccgatg ccttgaagta actctgcgaa gattcaagaa
     2761 cttgttcgcg gtcagtctcg gcgaccatgt gggctgcctg cgatgcatcg ttgacggctc
     2821 gtgccagaat ggccgcagcc cggcgatact ggcggtgcgg aactcaggac ctacctagaa
     2881 atggtttggc cacggcggtc atcgcgactc actgcccggg aacaaaacgg tgcgaagttc
     2941 cgggagcaat tgaccgagaa aatccgccat cccgtcttcc gtcgattgct cgcggtattt
     3001 ggtcaccgcg gagatcaggt acaacttgta aacgacagga tgaggcgcga acacgatgcg
     3061 ggggctctcg ggtacactcc aggtgccgct tgcgttccac gaccagtaga tcctcagtcg
     3121 atcagcagga atcgtttcac gccgctcgaa gtcggccatg aggaaatcgc cgccagactc
     3181 ggcgccaact tggatcttga ccggatgagc ctggacgatt tcgtagccgg cacccggata
     3241 acagatttca ggagtgtgga ccgagaccgg cccgggccta ccgcacacaa gcagcagagt
     3301 gaggctcttc cccgtcgctt cgtttttgta ggttcgcaac acaaaaccat cgaggcccgc
     3361 tgctaatatc tcgcggccat caagtgtcgc cggggtgcct ttccagttgc cgaccgtcat
     3421 cggtaagccc tcgacgcgaa atctcgccag ctcgagctca cttgacaatc cccagcgatt
     3481 tgtcctgtaa ccatggacaa gtcccgttgc aaggagggtg cagatcgcga cgacgatagg
     3541 gaacacgcgc gattaccgca cgtccgggac cgccggcttt gccaaggcgg gtcgattgtc
     3601 gatgggctcg accgtaacga aatttgggtc gggtagctcg ataatgagga ggctcaacaa
     3661 tcgggattcg atatagagtg ctgccaagga caacgg
//
LOCUS       DATAQL010000987         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4069385, whole genome shotgun sequence.
ACCESSION   DATAQL010000987 DATAQL010000000
VERSION     DATAQL010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4069385"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /gene="leuC"
                     /locus_tag="VHS97_17045"
     CDS             <1..1464
                     /gene="leuC"
                     /locus_tag="VHS97_17045"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX3449964.1"
                     /translation="DNMMCCTALHTASRFRGRLEMSETLFEKVWNRHVVKSTDLATLL
                     YIDRHLVHEVTSPQAFDGLRLAGRRVRRPDLTFATVDHNVPTDNQLDIRDPLSRKQVE
                     AMRANCREFGITLYDIQSGRQGIVHVIGPELGVTLPGTTIVCGDSHTSTHGAFGALAF
                     GIGTSEVEHVLATQTLWQGPRPDALGIEVTGRLGPGLEPKDIILAIIRKIGTGGATGH
                     VIEYYGPAISALSMEGRMTVCNMSIEAGARAGLIAPDDVTFEFITRVDRPFAPRGADL
                     DAAIADWRTLRTDDPADFDRHVTIDAGAIVPQVTWGTNPAMTVDVTGVVPRPEEVPFA
                     TPEAARSALQYMGLEPGTPISAIPVDVVFIGSCTNGRIEDLRAAARVLKGRKTAHSVQ
                     TLIVPGSEAVRHQAEAEGLDRIFKEAGAEWRQAGCSMCLAMNPDKLKPGQRSASTSNR
                     NFEGRQGPGGRTHLVSPAMAAAAAVTGHFTDVRQLLS"
     gene            1581..2273
                     /gene="leuD"
                     /locus_tag="VHS97_17050"
     CDS             1581..2273
                     /gene="leuD"
                     /locus_tag="VHS97_17050"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3449965.1"
                     /translation="MEPFVTHRGRLAVLDWNDVNTDLIIPARYLKKVERIGFGPLLFA
                     DKRYAPGGAPETDHPERHGPELPDFPLNNPAYNGATVLVVGRNFGCGSSREHAVWAVM
                     QAGYRAVIAPGKGVGFADIFESNAFQNGLLVLELEQNDWESIVAASRAQPSRAEVTID
                     LVLQTVTLHRPRIGSAPETETRFAFEVPESYRHRLLKGLDAIAETLVHESEITRHEQN
                     VPAWLRPQQVSV"
BASE COUNT          475 a          788 c          743 g          441 t
ORIGIN      
        1 gacaatatga tgtgttgcac tgcccttcat accgcgagtc gatttcgagg ccgattggaa
       61 atgtccgaga cgctctttga gaaagtgtgg aaccgccacg ttgtgaagtc gactgaccta
      121 gcaacgctcc tgtacatcga ccgacacctc gttcatgaag tgacgagtcc acaggctttt
      181 gacgggctgc ggctcgcggg acgccgcgta cggcggccgg accttacgtt tgcgaccgtt
      241 gatcacaacg ttccgaccga taaccagcta gacatccgcg acccgctctc acgtaagcag
      301 gtcgaagcga tgcgggccaa ctgccgtgag tttggcatca cgctctacga tattcagagt
      361 gggcggcagg gaattgtgca tgtgatcggt cccgaactcg gggtgactct gcctggcacg
      421 acgatcgttt gcggcgacag ccacaccagc actcacggcg cttttggagc gctcgccttc
      481 gggatcggca ccagcgaggt cgagcatgtc ctggcgactc agacgctctg gcaaggtccg
      541 cgcccggacg cgctggggat cgaggtgacc ggccggctgg gcccgggact cgagcccaaa
      601 gatatcatcc tggccatcat ccgcaagatc ggcaccggcg gcgcgaccgg tcatgtgatc
      661 gagtattacg gcccggcgat ctcggccctg tcgatggaag ggcggatgac ggtctgcaac
      721 atgtcgatcg aggcaggcgc acgggccggc ttgatcgctc ccgacgacgt gacattcgag
      781 ttcatcacgc gggtcgatcg cccgttcgcg ccccgaggag ccgacctcga cgcggcgatc
      841 gccgactggc ggacattgcg gaccgacgat ccagccgact tcgaccgcca tgtcacgatc
      901 gacgctggcg cgatcgtgcc ccaggttacc tggggcacca acccggccat gacggtcgac
      961 gtgaccggag tggttccccg acccgaggaa gtcccgtttg ccactccaga agcggcgcgc
     1021 agcgcgctcc agtacatggg cctagagccg ggcacgccca tcagtgcgat ccctgttgac
     1081 gtcgtcttca tcgggtcgtg taccaacggg cggatcgaag acctgcgcgc agctgcccga
     1141 gtcttaaagg gccgcaagac ggcccactcg gtgcagacat tgatcgtgcc tggcagcgag
     1201 gcagtccgcc atcaagccga agccgagggc ctcgaccgca tcttcaagga agccggcgca
     1261 gagtggcgcc aggctggatg cagcatgtgc ctggccatga atcccgacaa gctcaagcca
     1321 ggtcagcggt cggccagcac cagcaatcgc aactttgagg gccgtcaggg accggggggt
     1381 cgcacccacc tggtcagtcc ggccatggcc gccgcggcag ccgtcactgg ccacttcacg
     1441 gatgttcggc aactgctgtc ttaaactggc ttcgcctgca aacctcaaga tctgtttgag
     1501 accggaattc ggagatcggt tctccgaaat ccaatccggg aatccgcaat ctggaattcg
     1561 ccgatctgga acgttgaacc atggaaccct tcgtgaccca tcgcggccga ctggcagtgc
     1621 tcgattggaa cgacgtcaac accgacttga tcatccccgc ccgttatctc aaaaaggttg
     1681 agcggatcgg ttttggcccc ctgctcttcg ccgataagcg gtatgcgccc ggcggcgctc
     1741 cggaaacgga ccacccagag cggcacggcc ccgagcttcc cgatttcccg ctcaacaatc
     1801 cggcgtacaa cggggcgacc gtgctcgtcg tgggtcgaaa cttcggctgc ggatcgagtc
     1861 gtgagcatgc ggtgtgggcc gtgatgcaag ccggctatcg cgcggtgatc gccccgggga
     1921 aaggggtcgg attcgccgat attttcgagt ccaacgcctt tcaaaatggc ttgctcgtgc
     1981 tcgagctcga gcagaacgac tgggaatcca tcgtggcggc cagccgcgca cagccgagcc
     2041 gggccgaggt aacgatcgat ctcgtgctcc agacggtcac gcttcatcga ccgcgtattg
     2101 ggtccgcacc agaaaccgaa acccgcttcg ctttcgaggt ccccgagtct tatcggcatc
     2161 gactgctcaa ggggctcgac gcgatcgccg agacgctcgt tcacgaatcc gagatcacgc
     2221 gccacgagca gaacgtcccc gcttggctac gcccgcaaca agtgtcggtc tgacgtgtcc
     2281 tcatcgcggc cgcacgccgc gacgtaatcg ttcagcggaa ttccgcgacc gccaatacgc
     2341 gaacaatgag gaaaccagga aacgaggaag tgtccaaacc tgcctcctcc tgccttcctg
     2401 ggttcctcat tcggtccctt ccgcattcga ctgaacggtt actccgc
//
LOCUS       DATAQL010000988         3240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2237617, whole genome shotgun sequence.
ACCESSION   DATAQL010000988 DATAQL010000000
VERSION     DATAQL010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237617"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /gene="katG"
                     /locus_tag="VHS97_17055"
     CDS             complement(<1..1569)
                     /gene="katG"
                     /locus_tag="VHS97_17055"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX3449966.1"
                     /translation="MSTEAKCPFHHTAGGGTTNHDWWPNQLKVDLLHQHSAKSNPMGE
                     AFNYAEEFKTLDLAAVKKDLATLMTDSQDWWPADFGHYGPLFIRMAWHSAGTYRIGDG
                     RGGGGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGRKISWADLMILTGNVALETM
                     GFKTLGFAGGREDTWEPDRDVYWGSETTWLGGDNRYGKGKAGDDDGVIVADAELHGKE
                     HDRTDNGRNLENPLAAVQMGLIYVNPEGPEGKPDPVAAAHDIRETFGRMAMNDEETVA
                     LIAGGHTFGKTHGAAPASHVGPDPEAAGLESLGLGWSNSFGNGKAGDAITSGLEVTWS
                     STPTKWSNSFLSNLFGYDWELTKSPAGAHQWTPKNGAGAGTVPHAHDPSKRIAPSMLT
                     TDLALKFDPTYEKISRRFFENPDELADAFARAWFKLTHRDMGPRARYLGPEVPAEELI
                     WQDPIPAVNHPLIDAQDIASLKSKILASGLTVSQLVSTAWASASTYRGSDKRGGANGA
                     RVRLTPQKDWEVNTP"
     gene            1928..2371
                     /locus_tag="VHS97_17060"
     CDS             1928..2371
                     /locus_tag="VHS97_17060"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3449967.1"
                     /translation="MSDVLEPESLESSGPPLLDLFMTVVRVANWSTIVRWYTDTLGLV
                     PVLLDPQHEFAFLAAGQGRLGLQGIKGSSESGARSNVRLVFQVEDLDFQRQRLTEQGV
                     QVSAAIDNREEGYREIRLHDPVGNSLTLFAWLDSVPARRFSRDRD"
     gene            2468..>3240
                     /locus_tag="VHS97_17065"
     CDS             2468..>3240
                     /locus_tag="VHS97_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3449968.1"
                     /translation="MQVVRAAATGLLFITFSSIYSHFTRAQSPAPVENVSSLPPRTAT
                     DERKALHAPAGFEIQLVASEPDIHKPLNLAFDDRGRLWITDTVEYPYPAAAGTRPRDT
                     VKILSDFQADGRAGKIETFADGLNIPIGLLPLPSARAALVHNIPNIYLMTDLDGDGRA
                     DTRDVVYGIFGHKDTHGMTNAFTWGFDGWIYACHGFSNSSQVQGKDKRTISMNSGNTY
                     RMRPDGSHAEYFTHGQVNPFGLAFDALGNLYSCDCHSRPV"
BASE COUNT          558 a         1023 c          990 g          669 t
ORIGIN      
        1 aggcgtgttg acttcccaat ccttctgcgg tgtcagccga acgcgggcac cattcgcccc
       61 gccgcgcttg tcggagccac ggtacgtgga tgcggatgcc caagcggtcg acaccagttg
      121 cgagaccgtt aggccggaag ccaggatctt gcttttcagc gaggcgatgt cctgcgcatc
      181 gatcaagggg tggttgaccg cggggatcgg atcctgccag atgagttctt ccgccggaac
      241 ttccggcccg agataacgtg cacgcgggcc catgtctcgg tgcgtcaact tgaaccaggc
      301 gcgggcgaac gcgtcggcca actcgtcggg attctcgaag aaacgccgcg agattttctc
      361 gtaagtcggg tcgaatttca gggcgaggtc ggtcgtgagc atggagggcg cgatccgctt
      421 ggagggatca tgcgcgtgcg gcacggtgcc ggcgccggcg ccattcttcg gcgtccactg
      481 atgcgcaccc gccgggctct tcgtcagctc ccagtcgtag ccgaacaggt tcgacaagaa
      541 gctgttgctc cacttcgtgg gcgtgctgga ccaggtgact tccaggccgc tggtgatcgc
      601 gtcgcccgct ttgccgttgc cgaagctgtt gctccagccc aggcccagtg attcgagccc
      661 ggccgcttcc ggatcgggcc ccacatgaga tgcaggagct gcgccgtggg tcttgccgaa
      721 ggtatggccg ccggcgatga gcgccacagt ctcctcgtcg ttcatcgcca tacgaccgaa
      781 ggtctcgcga atatcgtggg ccgcggcgac cggatcgggc ttgccctccg ggccttccgg
      841 gttgacgtag atcaggccca tctgcacggc ggcgagcgga ttttcgaggt tgcgcccgtt
      901 gtcggtgcgg tcgtgctctt tgccgtgcag ttccgcgtcg gccacgatca cgccgtcatc
      961 gtccccggct ttgcccttgc cgtagcggtt gtcgccgccc agccatgtcg tctcgctgcc
     1021 ccaatacacg tcacgatccg gctcccacgt gtcttcacgc cctccggcga aacccaacgt
     1081 tttgaagccc atggtttcga gcgcgacgtt gcccgtgaga atcatcaggt cggcccagga
     1141 gatcttccga ccatacttct gcttgatcgg ccagagcagg cgccgcgcct tatccaggct
     1201 cacgttgtcc ggccaactgt tgagcggcgc gaaacgctgc tggccccggc cgccaccgcc
     1261 gcgcccatcg ccgattcggt aggtgccggc gctgtgccag gccatgcgga tgaacagcgg
     1321 accgtagtgg ccgaagtccg ccggccacca atcctgcgag tcggtcatca gcgtggcgag
     1381 atctttcttc accgccgcca ggtcgagagt cttgaactct tcggcgtaat tgaacgcctc
     1441 gcccatcgga ttggacttcg cggaatgctg gtgcagcaaa tcgaccttca actggttggg
     1501 ccaccagtcg tgatttgtcg tgccgccacc ggcggtgtga tgaaaggggc atttggcttc
     1561 ggtggacatg cgttgtctcc aattcttgaa tagtgaccgt ctacaagaac ctgtctgaaa
     1621 agttgctcca tgtcaattgg gagaagccgg caggaatgct gtgaatctgt tgcgaggcga
     1681 actgcttcgc cgctgctccg ggacatgcga tcctgtgcgt ggcttccatg ccttcggtat
     1741 ccttgcggcg cccttgcagc cctccctgta gccgcgctcc tggccagagc cgaggcgatg
     1801 cgatagccag cgcgtggcac gccgcttggt tgactttgat ctcacgaccc tctcgcttaa
     1861 cggtctgttg tctgacagaa taatcgtctc gatcctccct cctcggtcgt cgcgttaagg
     1921 cgtccagatg tctgatgttc ttgagcccga atcgcttgag tcgagcgggc caccgctcct
     1981 tgacctgttc atgactgtgg tccgggtggc gaactggtcg acgattgttc gctggtatac
     2041 ggacacgctc ggcctcgttc cggttctgct cgatccccag catgagtttg cattcctcgc
     2101 ggccggacaa ggacggctgg gcttgcaggg catcaaggga tcgagcgagt ctggcgcgcg
     2161 aagcaacgtg cgcctcgttt ttcaggttga ggatctggat ttccaacgcc agcggctgac
     2221 agagcagggg gtccaagtga gcgcggcgat cgacaaccgt gaggaaggtt atcgcgagat
     2281 ccgcctccat gatcccgtcg gcaacagcct gacgctgttt gcctggctcg actccgtacc
     2341 agcgcgtcga ttctcccgcg atcgagatta ggtcaaggaa aataacacgg gctgccggac
     2401 atgaacctcg ccctcggtcc aatccggcac acgcacgatc gagggaaaca atagccggag
     2461 atccgccatg caagtcgtgc gtgccgctgc gactgggctt ctgttcatca ccttttcgtc
     2521 gatttattcg cattttacgc gtgcccagag cccggcgccc gtcgaaaacg tctcgtcgtt
     2581 gccacctcgc acggcgaccg acgaacggaa agcgctgcac gcgcccgccg gcttcgaaat
     2641 acagctcgtg gcctccgagc ccgacatcca caagcccttg aatctggcct tcgacgaccg
     2701 cggccggttg tggatcacgg acacggtcga atatccctat ccagcggcgg caggcacccg
     2761 gccgcgcgat acggtcaaga tcctttccga tttccaagcc gacggccggg ccgggaagat
     2821 cgagactttc gcggatgggt tgaacatccc gatcggactc ttgccgctgc cgtcggcccg
     2881 ggccgccttg gtgcacaaca tccccaacat ctacctgatg accgacctgg acggcgacgg
     2941 ccgggcggac acgcgcgatg tcgtctatgg gatcttcggc cataaagata ctcacggcat
     3001 gaccaacgcc ttcacttggg gcttcgatgg ctggatctat gcgtgccacg gcttcagcaa
     3061 cagctcgcag gtccagggta aagacaagcg tacgatcagc atgaactcgg gcaacaccta
     3121 ccgcatgagg cccgatgggt cgcatgccga atatttcact cacgggcagg tcaatccgtt
     3181 tggattggcg ttcgatgcgc tgggcaatct ctattcgtgc gattgccaca gccggccggt
//
LOCUS       DATAQL010000989         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5138605, whole genome shotgun sequence.
ACCESSION   DATAQL010000989 DATAQL010000000
VERSION     DATAQL010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5138605"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1024)
                     /locus_tag="VHS97_17070"
     CDS             complement(347..1024)
                     /locus_tag="VHS97_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEX3449969.1"
                     /translation="MITRRVWIRRAFVIGIAGVGAEQLWRHGHDYVLPSQFFTVEPGK
                     IYRGAWQKPWPMRRIVNNYKIKTVLALAHPADHYLSVQERQLTTELGVKWIHIPIVDY
                     RGTNDQAAEDAISDLLDQAAAVLADPANYPIFFHCHHGVNRTSMVQIAYRTKYCGWNL
                     PQAAGEIERSIGLVKVNHGPDYRHMVSYYENRVLPLREQRSSRNSEKNPDLAARLDEP
                     DSATSKQ"
     gene            1499..>2770
                     /locus_tag="VHS97_17075"
     CDS             1499..>2770
                     /locus_tag="VHS97_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449970.1"
                     /translation="MRSDRPSGPISRIVGLYQMVLRRTLEHFFPDAQLEVSGDRSIID
                     WDGSADETYYRLHEDPDGLGVEIEWLGTLLGFQPGNPSPLLSTERRLVEVIVEAIDVR
                     FRGLFNQQLSHRFDRFQYQTEDWIVADFLNTVGAHRIPAALEALRVAALSTYENRRVS
                     TGALLLGTEHDPAAPAYKNLAGAPTFSARLTAIKGFHRLCDGVRTVFLVDRQGDLARM
                     VDITRWSDAAQGSEPLVHPCPRMYASHAKATRSGGHVCLVLTPSQEIKVFAEGTLMFS
                     FSDARWRLLDIPTKFAVWCEAVGRSRWPDLASSIFRAALNLCESRLGALFVVLRDPAQ
                     SIPQLIAPPDQISTEVVADDPQDPENLSPKHAKRALHHVVRGMNLADLEPSVLEAIAC
                     LDGAVVVDHDGRLLTFGAILRIAPESLELGRA"
BASE COUNT          547 a          834 c          797 g          592 t
ORIGIN      
        1 catcagcttt tggtccgcaa acacaaggat ctgttcgtag atccccggga catcaccaac
       61 acgcgcggga cgaatcgtca atcgaggaac ctccagtgcc ggcaaaaaaa accaggtgtc
      121 atggagagag atagagatgt aagttcaggt cggattgagc cttctgtccc tctcttaatc
      181 aatgtcgacc gactattgta atgctgagtg ctcgagaatc aaggaccggt cacagccaag
      241 agcgaatgaa ttctggcagg taacaaagtc cgaaccggca gccgcgcaat cccgtgcgtc
      301 ctcagacttc ggtctcttcg aaagttggct gaatcgcctg cggaatttac tgttttgatg
      361 tcgctgaatc cggctcgtcg aggcgagccg cgaggtccgg gtttttttcg ctgttccggc
      421 tggaacgctg ttctcgaagg ggaagcaccc ggttttcgta ataggaaacc atgtgccggt
      481 agtccggtcc atggttgacc ttgacgagcc cgatcgagcg ttcgatctcg ccggcagcct
      541 gggggagatt ccagccgcaa tacttggttc tataggctat ttgaaccatc gacgttcggt
      601 tcacaccatg gtggcagtga aaaaagatgg ggtaatttgc agggtcggca agcacggccg
      661 cggcttgatc gagcaggtca gagatcgcat cttccgcagc ctggtcgttg gtgccccgat
      721 aatcgacgat cggaatatga atccatttga ccccgagttc cgtggtcaat tgacgttcct
      781 gaaccgacag atagtgatcg gcggggtggg ccaaggccag gacggtcttg atcttgtaat
      841 tgttgacgat gcgtcgcatc ggccatggct tttgccaggc gccacgatag atcttgccag
      901 gctcgaccgt gaagaactgg ctgggtaaca catagtcgtg tccgtgacgc caaagttgtt
      961 ccgcgccgac acccgctatc ccgatcacga atgctcgacg aatccagaca cgtcgggtga
     1021 tcatcgcgcc caactccctc tcttacaaga ccttgtaacg tggcgcctcg atctgtctcc
     1081 ggcaatcgca cggttgtccg attggcgtcg ggggcggatc gtagcaggag aacttaccca
     1141 cgacaagtcc gatagcattg cgccccacgt gctgggttct ccgatctttt ttcatcgtcc
     1201 gacgatcacc ctgcccgtca atctttgtgt caaccaggca agctatagtc ccaaaaacgc
     1261 aatgataacg ttagaattcc tcattcaagc accgaggtcc ggcagtcttc gctctctggg
     1321 caagccgcgc gattcgtctc gggtaccgcg atcgaggact gacaagctgg atctctcgaa
     1381 gccttgacta gataggtcgc tcatggcaag aaggcgccct cgatggcccg gttcactctt
     1441 gatggatcgc ggtgcgtcgt cgtctcgtat tcgccaaact ccggagcctg cattttcaat
     1501 gagaagcgat cggccgagcg gcccgatctc ccggatcgtg ggcttgtacc agatggtgct
     1561 gcggcgcacg ctcgagcact ttttcccgga cgcacagctc gaggtctcgg gtgatcggag
     1621 catcatcgac tgggatggct ccgccgatga gacctattac cgcctacatg aggatcccga
     1681 cggcctggga gtcgagatcg agtggctcgg aacgcttctc ggatttcagc cgggcaaccc
     1741 ctcgccgctc ttgtccaccg agcgccgcct cgtcgaagtg atcgtcgagg cgatcgacgt
     1801 tcggtttcgg gggctgttca accagcagtt gtcccatcga tttgaccggt ttcaatatca
     1861 gacggaagac tggatcgtcg ccgattttct caacacggtg ggcgcccatc ggatccccgc
     1921 cgccttggag gcgctccggg tcgccgcgct atcgacttat gaaaaccgaa gagtgtcgac
     1981 cggtgcactc ctcctcggca ccgaacacga cccggccgcc cctgcgtaca agaacctggc
     2041 gggggcaccc accttcagcg cacgcttgac agcgatcaag ggattccacc gcctctgcga
     2101 cggtgtgcgg acagtctttc tggtggaccg ccaaggcgac ctggcgcgga tggtcgacat
     2161 tactcgctgg tccgacgccg cccaggggag cgaaccgttg gtccatccct gcccgcgcat
     2221 gtacgcgagc cacgccaagg ccacgcgatc gggtggacac gtctgccttg tgttgacccc
     2281 ctcccaagaa attaaggttt tcgccgaagg cacgctgatg ttcagcttca gcgatgcccg
     2341 ctggcggctg ctcgacatac ccaccaagtt tgcggtctgg tgcgaagctg tgggacgatc
     2401 ccgatggccc gatctggcct cgtcgatctt tcgcgccgcg ctcaatctat gcgaatcgcg
     2461 attgggggcg cttttcgtcg tcttgcgcga tcccgcccaa tcgatacctc agttgatcgc
     2521 tccgcccgac cagatctcga cggaagtcgt cgccgacgac ccccaagacc ccgagaacct
     2581 atctcccaaa cacgccaagc gggccctcca ccatgtcgtg cgaggaatga atctggccga
     2641 tctcgaacct tccgttctgg aggcgatcgc gtgcttagac ggcgccgtcg tagtcgacca
     2701 tgacggccgg ctcttgacat tcggggcgat tctgcggatt gcgccggaaa gtctcgagct
     2761 gggccgcgcg
//
LOCUS       DATAQL010000990         8167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5138636, whole genome shotgun sequence.
ACCESSION   DATAQL010000990 DATAQL010000000
VERSION     DATAQL010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5138636"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2523
                     /locus_tag="VHS97_17080"
     CDS             <1..2523
                     /locus_tag="VHS97_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3449971.1"
                     /translation="KTENSAPLVQLADLELARNQLDQAQEVIAKIRNRWRESATSDVL
                     EAQLALKRGQTAVAIDHFEAALKKDPENKIVQYWKAQLDGQNGAVADATKSLEAIVRN
                     KPVKEVDAGTSLMSAAQSALASLSLRTGALDDAIRRFEELRRSNQNGTLTKEDRWHLI
                     TAYAMRGQWPAAKREIGTLLNDPKNPPSDDERVRGANLYRQDGDEAAAVAQLDYVLQI
                     NPTHTAAVVTRSFIMLKAKHHDQATAILHKAIELVTQKKDMVPPVFFLMLATVANDQA
                     PSAEGLKQAMAILDQGLERLPDSLELVQAKYAALKATGQSKVAIELVEAKAKAYPAGQ
                     FRRELVNVYREQRLYDRAAKLLRELLEESPNDTNLAAALVQVVSLDAAEAGAANQPDR
                     QRDLNDRAATMIRQYRERFPNSIVFLQAECDLVARRGDFSRAIELTREIDKVSKTSPM
                     GALLRARLYAASGQTRDLAQAYKDALDRNPRQIDLRVLLGQTKLKLGQADEALRQANI
                     VLDAEKNRPDALLLQARALAESGTTMTEKDQQQQAAVARLVRATKANPTFEEAFHTLA
                     ELHLKRNNRAAALAVLKENLKANPTDATAAAKLIEILAQKRPDDKPVSASDLEEAKRI
                     AHELASRDPNGPMILAVAAGLHKARQLELALPFAVDAAAKLDSPSAHLNLGDLLLTLA
                     ETQTDPQRGASFERAVSEYDKVLKKQPTSIEAVNNKAWILHSYLGQTRQALELVLALQ
                     KRVNAAALPGEFYDTLGSIQESVGKTREAEQSYLSGLKKAPEHAVLNFHFGKMIAADS
                     SRGSKARTHLKKALDSRDRLSLMMTQEATRLLEQIDGNKRVQ"
     gene            complement(2561..2758)
                     /locus_tag="VHS97_17085"
     CDS             complement(2561..2758)
                     /locus_tag="VHS97_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449972.1"
                     /translation="MEPTKRELRRLKKEIKRAGSQRRRREWKRDLRENPEEAAYSEEN
                     FGRYSTAELNGIDHDSTRRRD"
     gene            complement(2784..3632)
                     /locus_tag="VHS97_17090"
     CDS             complement(2784..3632)
                     /locus_tag="VHS97_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3449973.1"
                     /translation="MTESSSLVRSFVASDGYPLHVRIWPAESQPRGQVVVLHGVQSHG
                     GWYHGLGKSLAAAGYTVSFPDRRGSGANALDRGHTRTARRLNLDLAEWLRVVRSEDPR
                     LPVALAGISWGGKLAMIAAARYPKLVDAVALICPGLHPRLGVTRKARLQIAWAFLTNR
                     RKIFPIPLSDPALFTANPQGQAFIAADPYSIREGTAGLMAASFIIDRLVALAPRRVHQ
                     PALLMLAGQDRIVDNKKTLAYFERLASTKRQVIEYPDGHHTLEFDPEPDRYAADLVTW
                     LNYHLR"
     gene            complement(3625..4896)
                     /locus_tag="VHS97_17095"
     CDS             complement(3625..4896)
                     /locus_tag="VHS97_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX3449974.1"
                     /translation="MLLTAAAATTKFSLNGSDKNVETDGIGNQGMSIASLKLFKVAIP
                     LKKVIKHASFERSESENLVVRVTLSDGIVGYGEGVPRSYVTGETPESAIAALEAHDWS
                     AIMGRPSSFPQVVERLETLTLPEIEGDPRGMAGNAARCALEMAILDAYGLRFGESIGR
                     AVELAKVEGLKRLAQPQRVRYSAAITAESARSERISALKIRIYGFHQVKIKVGTRGQD
                     DGRRLERMRKLLGRRMDIRLDANEAWAVAELVDRVQPLLRFGPTALEQPVPHADVEAL
                     AELRPRLGVPVMLDESLCGYPDALSALELRTADLFNVRLSKCGGILPSLRIIGLAHRS
                     GLGVQLGCHPGETALLSAAGRHVASRVGGLRYVEGSYDRHILAANLTTQDFTFGYGGR
                     ARPIKGPGLGVKVDPAALEAITLSSREVRYD"
     gene            complement(4898..5299)
                     /locus_tag="VHS97_17100"
     CDS             complement(4898..5299)
                     /locus_tag="VHS97_17100"
                     /inference="COORDINATES: protein motif:HMM:NF023276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HEX3449975.1"
                     /translation="MLRIGVDEVAVPESVIAEIHGHGSDDPTVKAIESLDWLQIRPAG
                     KVASVVALWDLGPGESAVLSLALAEEFSWAVIDDWQARRCARSLGIRCIGTLGLVLLA
                     KQVGRIPAARPVVEQLRMSGTISSCTNQRSR"
     gene            complement(5277..5609)
                     /locus_tag="VHS97_17105"
     CDS             complement(5277..5609)
                     /locus_tag="VHS97_17105"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HEX3449976.1"
                     /translation="MAAVTIEIPDDAFATLHRSPKELSYEMRLAAAMIWYTQGRISHE
                     QAARFSGLSRIAFIDALAEASLPAFHVDVDELMEEVECAGQAIREHVTPGVPSSGESP
                     GNAQDRSR"
     gene            complement(5842..7227)
                     /locus_tag="VHS97_17110"
     CDS             complement(5842..7227)
                     /locus_tag="VHS97_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3449977.1"
                     /translation="MVVHYRRPVWGVFLGFVWATLIGVAADAHDIPNQRVDRSIQVTL
                     EPGLLHIDYEVGLTELTLTQDLRSLMGGLPGGDRSAWLAQYGQVTAPLNAKGFLVAVD
                     GQPVSLAGEKYDLVIEEHPRYTFHYHAKLGIHGRLSVRDRNYSSSEGSSRLAVRGLGG
                     VKIDGDQLPAVVDQIAIRPVWQLSDEEERRTKQVEVSFETRPTADMTAAEDRRLSAHD
                     TDDTHGKQGRAKFPASDQITRLSDLLDIAGRRSWVFLGLIALLLGSAHAIQPGHGKSL
                     VTAIALGPHSRFYQTALLGLATTLAHMSSVLCLALILWITGATRVATFHQSLTRIAGF
                     VIAAAGVWRIGRYLGGHGEHASEDIHVAEMSYVEILGLGLAGGIVPCWDAVGLLVLAA
                     TLGRLAEGVGLVLAFSAGMGLVLLAVGWLAWKFKSKMVVANGGAKWQRRLGLVCGLIL
                     ASLGIYLFLQA"
     gene            complement(7242..7625)
                     /locus_tag="VHS97_17115"
     CDS             complement(7242..7625)
                     /locus_tag="VHS97_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3449978.1"
                     /translation="MASAQPFSLLITDDDPAARETLRDIFAPQGFNTIMAESGEEAID
                     LIRDHEVHLALMDMHLPRLSGLETIAIVRQIKGVIPAILITADQDDNLMRRALSEHAF
                     CVLAKPVSKHVVIYVVRKALEKYYN"
     gene            complement(7838..>8167)
                     /locus_tag="VHS97_17120"
     CDS             complement(7838..>8167)
                     /locus_tag="VHS97_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449979.1"
                     /translation="ELSYRGLEKAAGLDRPCHHFVRKSTSGDEWNVYLDPSSMLPRMV
                     FAEDSRHELIERYNYREVHENPDDLAAAGAFEPDQRWGESKGLLGRLARAASNSNLPA
                     TNASTTR"
BASE COUNT         1745 a         2546 c         2364 g         1512 t
ORIGIN      
        1 aaaaccgaga attcggctcc cctcgttcaa ttagccgatc tcgagctcgc gcggaatcag
       61 cttgaccagg cccaagaggt catcgccaag atccgcaatc gatggagaga atccgccaca
      121 agcgacgtgc tcgaagccca gctggctctg aagcgaggtc agacggccgt tgccatcgac
      181 cacttcgagg cggcgctcaa gaaagacccc gaaaacaaga ttgtccagta ctggaaagcg
      241 caactggatg gccagaatgg ggccgtggcc gatgccacca aatcgctgga ggcgatcgtc
      301 aggaacaagc cggtcaagga agtcgacgcg ggcacgtcgc tcatgtcggc ggcccagtcg
      361 gccctcgcca gtctatcgtt gcgcacgggc gcactcgacg acgcaattcg ccggttcgag
      421 gaactgaggc gtagcaacca gaacggcacg ctgaccaagg aagaccgctg gcacttgatc
      481 actgcgtacg cgatgcgggg acaatggccc gcggccaagc gtgaaatcgg cacgctcttg
      541 aacgatccca aaaatcctcc ttcggacgac gagcgcgtcc gcggcgctaa cctctaccgt
      601 caagacggcg acgaggccgc cgccgtcgcc cagctcgatt acgtgcttca gatcaatcca
      661 acccacaccg cggccgtcgt gacccgttcg ttcatcatgc tcaaggccaa acatcatgac
      721 caggcgaccg cgatcctgca caaagcgatc gagctcgtga cccagaagaa agacatggtg
      781 ccgccggtct ttttcttgat gctggcgacc gtcgcgaacg atcaggcacc gagcgccgaa
      841 gggctcaagc aggccatggc aattctcgat caaggcctcg agcgcttgcc cgactcactc
      901 gagctggtgc aagccaagta cgcggcgctc aaggcgaccg gccaatccaa agtcgcgatc
      961 gagctggtag aggccaaggc caaggcatac cccgcggggc agtttcgtcg cgagctcgtc
     1021 aacgtctacc gtgagcagag actctacgac cgcgccgcga agttgctccg cgagctgctc
     1081 gaggaatcgc ccaacgacac caacctggcg gcggccctgg tacaggtcgt ctcgctcgac
     1141 gcggccgagg ctggtgcggc gaaccagccc gaccgtcagc gtgacctcaa tgaccgggcc
     1201 gctacgatga tccggcaata cagggaacgc tttcccaata gcatcgtatt cctgcaggcc
     1261 gagtgtgacc tcgttgctcg tcgcggtgat ttctcccggg ccatcgagct gacgcgcgag
     1321 attgacaagg tgtcgaagac ctcgccaatg ggcgcactct tgcgggctcg actttacgca
     1381 gcttcaggac aaacgcggga cctggctcaa gcctacaagg atgctctcga ccgaaacccg
     1441 cgccagatcg acctgcgcgt gctgctgggc cagaccaaac tcaagctcgg gcaagccgac
     1501 gaggccctcc gtcaggcaaa catagtgctc gatgccgaga agaatcggcc cgacgcctta
     1561 ttgcttcagg cgcgtgctct tgccgaatcc ggtacgacga tgaccgagaa agaccagcag
     1621 cagcaggcgg ctgtcgctcg gctggtaagg gcgaccaaag cgaatcccac ttttgaagaa
     1681 gcgtttcata cccttgcaga gcttcactta aagcgaaata accgcgccgc cgccctcgcg
     1741 gtcctcaagg agaatctcaa ggccaatccc accgacgcca cggccgcggc aaaactgatc
     1801 gagatcttgg cgcaaaagcg ccctgatgac aaaccagtaa gcgcgtcgga cctggaagaa
     1861 gccaagcgaa tcgcgcacga gctcgccagc cgagacccga acggtccaat gatcctggct
     1921 gttgccgcgg gcttgcacaa agctcgtcag ctcgagctgg cgcttccttt tgccgtcgac
     1981 gccgcggcca agcttgacag tccgtcggcc cacttgaacc tgggcgacct gcttctcact
     2041 cttgccgaga cccagaccga cccgcagcgc ggggcctcct tcgaacgtgc cgtatctgaa
     2101 tatgacaagg tgctgaaaaa acagcccacc tcgatcgagg cagtcaacaa caaagcatgg
     2161 attctgcact cgtacctggg acagacgcgg caagcgctcg agctggttct ggccttgcaa
     2221 aaacgcgtca atgcggccgc gctgcctggc gaattttacg acactctagg ctcgatccag
     2281 gagtccgtcg gcaagacccg cgaagccgag cagtcgtact tgagtggctt gaaaaaggcg
     2341 cctgagcacg cggtcttgaa cttccatttt ggcaagatga tcgcagccga cagcagtcgc
     2401 gggagtaaag cacgcacgca cctgaagaag gcgctcgatt cccgcgaccg ccttagcctt
     2461 atgatgactc aggaggccac caggctattg gagcaaatcg acggcaataa gcgcgttcaa
     2521 taacaggctg cccgcccgtt tgggagaccc gcccggcaat tcaatcgcgc cggcgggtac
     2581 tatcgtgatc gattccgttg agctcggcgg tcgaataacg accgaagttc tcctcggaat
     2641 aggctgcctc ttcggggttc tcacgcaggt cgcgtttcca ctccctgcgc cggcgctggc
     2701 tccccgcgcg cttgatctct ttcttcagcc ggcgaagctc gcgtttggtc ggttccatgg
     2761 ttcaggcatc ctggtccctt ggtttaccgg agatgatagt tgagccatgt aacaagatcc
     2821 gcagcatagc ggtcaggttc ggggtcgaat tcgagagtgt ggtgtccatc gggatattcg
     2881 atcacttgcc gcttggttga cgccagcctc tcaaagtagg ccagtgtttt tttattgtca
     2941 acaatgcgat cctgcccggc cagcatgagc aacgcgggct ggtggacgcg acgcggcgcg
     3001 agcgcgacca agcggtcgat gatgaaactg gccgccatca atcccgcggt gccttcgcgg
     3061 atactgtagg gatccgccgc gataaacgcc tgcccctggg ggttggcggt gaagagcgcc
     3121 gggtcggaaa gggggatcgg gaaaatcttt ctgcgattgg tgagaaaggc ccacgcgatc
     3181 tggagtcgcg ctttgcgggt gacccccaac cggggatgaa gcccgggaca tatcaaggcc
     3241 acagcatcaa ccagtttggg gtaccgagcc gcggcgatca tcgccagctt tccgccccaa
     3301 ctgatgccgg ccagtgcgac cggcagacga ggatcctcgc tgcgcactac tcgcagccac
     3361 tcggcgaggt cgaggttgag ccgccgcgcg gttcgggtat gtccccgatc aagcgcattg
     3421 gcccccgagc cgcggcgatc aggaaatgag accgtgtaac ccgccgcggc cagcgacttt
     3481 cccaacccgt gataccagcc tccgtggctt tgaacgccgt gcaagactac gacctggccg
     3541 cgcggctggg actcggcggg ccatattcgc acgtgtagtg ggtagccgtc cgacgccacg
     3601 aacgatcgaa ccaaggaact cgattcagtc atagcgaacc tctcggctcg acagcgtgat
     3661 cgcctcgagt gcggccgggt cgactttcac tcccagtccg gggcccttga tagggcgagc
     3721 acggccaccg tagccgaagg tgaaatcctg agttgtcaga ttggcggcca ggatgtgccg
     3781 gtcgtacgag ccctcgacgt atcgcagacc accgacacgg ctggcgacgt gccgccccgc
     3841 cgcggaaagg agtgctgtct cgcccggatg acagccgagc tgtacgccca gacccgagcg
     3901 gtgcgccagg ccgatgatgc gcagtgaggg aaggattccg ccgcacttgg ataaccggac
     3961 gttaaaaaga tcggcggtcc gcagttccag agctgacagg gcatcaggat agccgcaaag
     4021 ggactcgtca agcatgaccg gcactcccag tcgtgggcgg agctcggcca gggcttcgac
     4081 atctgcgtgc ggaacgggct gttcgagggc agtaggaccg aaacgtaaga gcggttgcac
     4141 gcgatcaacc agctcggcaa ccgcccacgc ttcgttggcg tcgagccgga tatccatccg
     4201 ccgacccaag agtttgcgca ttcgctcaag ccgccggcca tcatcctgtc cccgagtgcc
     4261 gactttgatt ttcacctggt ggaagccata aatccggatc ttcaaggccg agattcgctc
     4321 ggaacgcgcg gactcggcgg tgatcgccgc gctgtagcgc acccgctggg gctgcgccag
     4381 acgctttagt ccctcgacct tggccaactc aacggctcgc ccgatcgact cgccaaatcg
     4441 taacccataa gcgtccagga tagccatctc gagagcgcac cgtgcggcat tgcctgccat
     4501 gccgcgggga tcgccctcga tttcgggcag cgtcagcgtt tccagccgct cgaccacctg
     4561 cggaaagctg gatggccgac ccatgattgc ggaccaatca tgggcttcga gtgcggcaat
     4621 ggccgattcc ggggtctcac cggtcacgta cgaccgtggc accccttcgc cgtagccgac
     4681 gattccgtcg gacagtgtaa cccgcacgac aagattttcg ctctccgatc gctcgaacga
     4741 agcgtgcttg atcactttct tcagtgggat cgcgaccttg aagagcttca agctggcgat
     4801 gctcattccc tgatttccga tgccgtcggt ttcgacattc ttatcggaac cgttcaagga
     4861 gaatttggtc gtggcggccg ctgctgtcag gagcactcta tctgctccgc tggttcgtgc
     4921 aactcgatat tgtcccagac atgcgtagct gctccacgac gggccgggct gccggtattc
     4981 gaccgacctg tttggccaag agaacaagtc caagagtacc gatgcacctg atccccagag
     5041 accgggcgca gcgccgggcc tgccaatcgt caatcacggc ccaagaaaac tcttcggcca
     5101 gagcgagcga cagaacagct gattctccgg gaccaaggtc ccagagagcg accaccgacg
     5161 cgactttacc cgcaggccgg atctgtagcc aatccaaaga ctcgattgcc ttaaccgtgg
     5221 gatcgtcgga accgtggcca tggatttccg cgatgacgct ctcgggaacc gctacttcat
     5281 cgactccgat cctgagcatt tccaggagat tctcccgagc taggaacacc aggggtgacg
     5341 tgttcacgaa tcgcttgccc ggcacattcg acctcctcca tcaattcgtc cacatcgacg
     5401 tggaacgcag ggagacttgc ctccgcaagc gcgtcgatga aagcgatccg gctcaggccc
     5461 gagaatcgag cggcttgctc gtgagaaatg cggccttggg tataccaaat catcgccgcg
     5521 gccagccgca tttcatagct cagttcctta ggagagcgat gcaaggtagc gaatgcgtcg
     5581 tctggaattt cgatcgtgac agcggccata gtcttgtcct cgcgtgccca cttttgggtc
     5641 acagaaactt catcgacgcc ccggttcctc tatctcagat cagtgccaat tcattctatc
     5701 cggttgcagg ctgtgaccac cacttcgaga cggcgattca gggtgcgaga gaggccggat
     5761 aaaacctttt cggcccaaag tgaaaaagca tcgcagatac ttatatacgg agacgaccgg
     5821 cgcaaaaatc ggcccggctt ctcaagcctg aaggaagaga taaatcccga gcgatgcaag
     5881 gatcagtccg caaaccagtc caagccgtcg ctgccacttc gcgccgccgt tcgcgacaac
     5941 catcttggac ttgaacttcc atgccagcca gccgacagcg agcagcacca agcccatgcc
     6001 cgcgctgaag gcaagtacca agcccacacc ctccgccagc cggccgagtg tcgccgctag
     6061 caccaagagc ccgacggcgt cccagcatgg aacgatcccc ccggccagcc ccagtcctag
     6121 aatctcgacg taactcattt ctgcgacgtg aatgtcttcg gaggcgtgct cgccatgacc
     6181 gcccaggtat cgaccaatcc gccacacacc ggccgccgcg atcacaaatc cggcaatccg
     6241 ggtcaaagac tgatgaaacg tcgcgacgcg cgtcgcgccc gttatccaca agatcaaagc
     6301 gaggcacaac acactgctca tatgggcaag agtcgtagcg agtcccagga gagccgtctg
     6361 ataaaatcgc gagtggggac caagcgcaat cgcggtcaca agcgacttac catgcccagg
     6421 ctggatggca tgtgccgacc cgagcaatag cgcgatcaaa ccgaggaata cccacgatcg
     6481 tctcccggcg atgtccagca aatccgagag ccgcgtgatc tgatcgctcg ctgggaattt
     6541 agcacgtcct tgttttccgt gagtgtcatc cgtgtcgtgc gcgctgagcc ggcgatcttc
     6601 cgcggccgtc atatccgcgg tcggcctggt ctcgaaactg acttccactt gtttggtgcg
     6661 gcgctcttcc tcatcactca attgccagac ggggcgaatg gcaatttgat caacgaccgc
     6721 cgggagttga tcgccgtcga ttttgacccc gcccaagccc cgcacggcca aacggctcga
     6781 tccttcgctc gaactgtagt tgcgatcgcg gaccgaaagt cggccatgaa tgccaagctt
     6841 cgcatggtag tgaaacgtgt agcgcggatg ttcctcaatg acgaggtcgt atttttcacc
     6901 agcaagtgac accggctgac cgtcgacggc aaccagaaat cccttggcgt tgagcggcgc
     6961 cgtcacctgg ccgtattgag cgagccatgc tgagcgatcc ccacccggca ggccgcccat
     7021 gagcgatcgc aggtcttggg tgagagtcag ctccgtcagg ccgacctcgt aatcaatgtg
     7081 caaaagtcca ggctcaagtg tgacctggat cgatcgatcc accctctgat tgggaatatc
     7141 gtgggcgtca gccgcgaccc caatcaaggt ggcccacaca aatccgagaa aaacgcccca
     7201 gacagggcgg cggtagtgaa cgaccacggc gagatcggcg atcagttgta gtatttctcg
     7261 agcgccttac gcacgacata gatcacgaca tgcttgctaa ccggcttggc caaaacgcaa
     7321 aacgcatgtt cggagagagc gcggcgcatg agattatcat cctggtctgc cgtgatcaga
     7381 atcgccggaa tcactccctt gatctggcga acgattgcga tcgtttccag tcccgagaga
     7441 cggggcaagt gcatgtccat gagcgcgagg tgcacttcat ggtctcgaat caggtcgatc
     7501 gcttcctcgc cgctctcggc catgatcgtg ttgaaaccct gcggagcgaa aatatcccgc
     7561 agcgtttccc gagcggccgg gtcgtcgtcg gtgatgagga gcgagaaagg ttgagccgag
     7621 gccatgggag acggactcgc tgtgaaccga acgcttgaac ggataaccag ttgatccttt
     7681 tgcctgcact cttcgcgccg ttatcaattg cttttccaac tctatttgag gcagcagtac
     7741 taacttattg caagattgat gtttacattt tgcgttccat cggcgttctg ccggtggttt
     7801 tgggcaacaa gagcctgttt gtgcgtcaca ttggcagtca gcgcgtcgtt gaagcgttgg
     7861 tcgctggcag gttcgagttc gacgcagccc gtgccaggcg gcccagaagt ccttttgatt
     7921 cgccccagcg ctggtcaggc tcaaaggctc ccgccgcagc caaatcatcg gggttttcgt
     7981 ggacttcacg atagttgtac cgctcgatca gctcatgtcg cgaatcctcg gcgaaaacca
     8041 tgcgcggcaa catcgagctc gggtcgagat aaacgttcca ctcgtcaccc gaggtggatt
     8101 tgcgcacaaa atgatggcag ggtcggtcga gtccggccgc cttctccagg ccccgatagc
     8161 tcaattc
//
LOCUS       DATAQL010000991         5002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4985007, whole genome shotgun sequence.
ACCESSION   DATAQL010000991 DATAQL010000000
VERSION     DATAQL010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4985007"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VHS97_17125"
     CDS             complement(<1..668)
                     /locus_tag="VHS97_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX3449980.1"
                     /translation="MDLRETLSALLPPRRDDEPPTLRQDILDELGDHLGCAYNRELLR
                     GADSQLARQRVLERFGDPAAVARRLWFDAMKGIIMAQRVVIVTCSLVTMASLSLVGLV
                     WMQSSRAAAQAMEERRKFSDALAQSQIASKDMLKTLSEMSDAIRNPRSPDWNPVTIKL
                     TEETTNGAPVVGCSISLSQRGGANATINRTTDDSGVADFGLLHPGTYSYSLCEGQNFR
                     SPIGS"
     gene            complement(853..1194)
                     /locus_tag="VHS97_17130"
     CDS             complement(853..1194)
                     /locus_tag="VHS97_17130"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3449981.1"
                     /translation="MDSKFLGSAVEMLILEVAGQGQTYGYELAQTVAERSNGYFELKE
                     GSLYPALHRLERQKLLRSFWREGDGRRRKYYELTEAGRLDLASRKKSWLSFAAGVNGV
                     LGTNRVIAAGT"
     gene            1353..1712
                     /locus_tag="VHS97_17135"
     CDS             1353..1712
                     /locus_tag="VHS97_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449982.1"
                     /translation="MPMYIYDPDDIDLTGFQRLNDQLHEISEINGWISPLLGIRFKVT
                     GNNLEIYGPDGRPFLTYQEIADERDRAEKERDQAALERDRAALERDQMALERDVERQR
                     ADRMAARLRTMGIEPIE"
     gene            1889..2557
                     /locus_tag="VHS97_17140"
     CDS             1889..2557
                     /locus_tag="VHS97_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449983.1"
                     /translation="MDIVIRSLYGWWFLLAAIAGSMLAMAGASVGGTIANPQSAAVHR
                     TSGGGSTAAADSLPVSKSGRDAAAAQRSGPDAEHFCFGPYASGCTCPWFPLTATLDAG
                     RLLQKCIQAQPGTAARIVPPARMRVTRLQNGSILCFRYRPHTRIVAVSLNGSDDTASD
                     GDENSDDDDSQDDQNGDDDSDSHIIAWIPATVLYLGAPARKSTISWIIPSFPPFLTLE
                     RLRC"
     gene            2927..4621
                     /locus_tag="VHS97_17145"
     CDS             2927..4621
                     /locus_tag="VHS97_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3449984.1"
                     /translation="MAAPTDDPTRPHEGPQIDHEIDQEMEHAARARLKMGWGQRAVVA
                     LLLTVVIVPPLAGFYSYFSGVPLHLLAARQDDEDKEAGGSTASTGISLVAGQAHTVEV
                     PDEVAATLGIRKGDKDSVTIAQPPSMMKPLVLPGSTALDPTHLARIRARFAPARVVEI
                     AQVQDPFRKAGRTEFRELRQGDSVSKGDLLGVFYSLDVGSKKNDLLDALVQLELDQHI
                     LDNAQAARQAVPEVFMLTAERAVQGDRNAVNRAINNLKLWEIPQDEIDALHAEAQKIC
                     SDKTAWFKTPEGRWVMREKQAHGGKVDPHKEAENPWGRVTLRAPFDGVVVERNLSVDE
                     MVVDNTINLFQIADVNRLLVLANCPEDSLPTLEALSQNQRRWTVQTVGANSATGLPGT
                     IDEIGYVIDPNQHTAIIKGYVDNPGRHIRAGQYVTATVQIPPPAGVVEIAVDALVDDG
                     RQSLVFVQPDADKHHFTMRRVHVTHRFDHSVFVRSTNYSKEEQLTALEAEEGLLPKEP
                     LRPGERVLVAGPVELKRVVLDLESRPKEKPTDRLAKAKPRSSSYFESPPKPKASKG"
     gene            4685..4879
                     /locus_tag="VHS97_17150"
     CDS             4685..4879
                     /locus_tag="VHS97_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449985.1"
                     /translation="MLAPVATDLLLIGIFVMIVGWVYGVFRRHVLVEHTQPPTSAHAA
                     ACSGRFVYRDKFFQQHDLRL"
BASE COUNT         1026 a         1547 c         1440 g          989 t
ORIGIN      
        1 gatccaatcg gggatcggaa attctggcct tcacaaaggc tataggagta ggtaccggga
       61 tgaagcaaac cgaaatcggc gacgcccgag tcgtcggttg tccgattgat cgtcgcgttg
      121 gccccgcctc gctgggacag actaatggag cagcccacca ccggagcccc gttggtcgtc
      181 tcctcggtca gcttgatcgt gacagggttc cagtcggggg agcgagggtt ccggatcgcg
      241 tcggacatct cactcaacgt cttcagcatg tctttactcg cgatctgcga ttgggccaat
      301 gcatcggaaa acttccgacg ctcttccatc gcttgagcgg ccgcgcggct ggactgcatc
      361 cacaccagac ccacgagcga caggctcgcc atcgtcacca acgagcaggt tacaatcacc
      421 acgcgctgcg ccatgattat ccctttcatc gcatcgaacc agagtcggcg agccacggcg
      481 gccggatcgc caaatcgttc cagaacacgc tgcctggcga gctgagaatc cgctccgcgg
      541 agcagttcgc ggttgtatgc gcacccgaga tgatcgccca gttcgtcgag gatgtcttgc
      601 cgcaaggtcg gcggctcgtc atctcgtcgc ggaggcaaca gtgccgaaag cgtctcgcgg
      661 aggtccatgg ttgattctta attcatcggt gaaaacaggc cgagtctacc atcggaagac
      721 ctgcggtcgg gcgttccggc ggggtcggag atccgcgcca aacgctggag tctaccaatc
      781 gggagacctg cggtcgggcg ttccggcggg gtcggagatc cgcgccgatc gcggcgtgat
      841 cgagcaaccc gatcacgtac ccgccgcgat gactcggttc gtcccgagaa caccgttgac
      901 gccggccgca aagctcaacc aggatttctt gcgcgaagcc agatcaaggc gacctgcttc
      961 ggtgagttcg taatacttcc gccgccggcc atccccttcc cgccaaaacg accgcagcag
     1021 tttttgccgc tcaagccgat gcagtgccgg gtaaagcgat ccttctttca actcaaagta
     1081 gccattcgac ctctccgcca ccgtctgcgc cagctcgtag ccgtaagtct gcccctggcc
     1141 ggccacctcc aggatcaaca tctcgacggc actaccgaga aatttcgagt ccatacctcg
     1201 acctcctatg cattgctatc taatacatag acagccgagg tatcgttgtc aagtcgtcca
     1261 ttttggaatt tcctggcgtc gttcgcgccg gcggcagggg cgggtaaaat agtaacctgg
     1321 tgcggggtgc tggtggcgat tccgagcgga tcatgccgat gtacatctac gatcctgacg
     1381 atatcgacct aaccggcttc cagaggctga acgatcagct tcatgaaatc agcgagatca
     1441 acggttggat cagcccgctt ttgggaatcc gcttcaaggt cacaggcaat aacttggaga
     1501 tctacggtcc cgatggccgg ccgttcttga cttatcaaga gattgccgac gagagagatc
     1561 gggccgaaaa ggaacgcgac caggccgcac tggaacgcga ccgggccgct ttggaacgcg
     1621 atcagatggc actcgaacgc gatgtggagc gccagcgtgc cgaccgcatg gccgccaggc
     1681 tacgcacgat gggcatcgag ccgatcgaat agctggtcca caagtcagtc gctggtctac
     1741 cgttcgaaaa tctcaccacc gtcaaccgct cgtttgtgtg cgaacgatca acaagccagg
     1801 tacgtagcaa cgttagcggc ttgcatgata tgcggattac tgctacgatg cgttttctcg
     1861 tcttgaaact cctcgatcaa ggttgggtat ggacatcgtt atccgctcgc tgtacggatg
     1921 gtggtttctc ctcgccgcaa tcgccgggtc gatgctggca atggccggcg cgtcggtcgg
     1981 cgggacgata gctaatcccc agtctgccgc ggtccatcgt actagcggag gtggatcgac
     2041 tgccgccgcg gacagcctgc cggtttccaa gagcggacgt gacgccgcgg ctgctcagcg
     2101 atccgggccg gacgcagaac atttttgctt tgggccatac gcctccggct gtacgtgccc
     2161 ctggtttccg ctgaccgcaa ccttggatgc gggacgcctt ctccaaaagt gcattcaagc
     2221 ccaacccgga actgccgctc gcatcgtgcc cccggcccgc atgcgcgtga ctcgattgca
     2281 gaacggttcg atcctttgtt ttcgctaccg tccccatacc cgcatcgtgg ccgtctcgtt
     2341 gaatggcagc gacgatacgg cgtcggacgg cgacgagaat tccgacgacg acgattcgca
     2401 ggacgaccaa aacggcgacg acgactcgga ctcccatatc atcgcgtgga tcccggcgac
     2461 cgttctctac ctgggcgccc ctgcaagaaa atccacgatc tcgtggatca tcccctcctt
     2521 tcctcctttc ttgacgttag agcggcttcg ttgttgagcc cgtcggggca atcaccgcag
     2581 cgataacaag gatacgcacg ccgccggtcc gcatcgcgcg gaaatcgcgt tgcgctggct
     2641 cggtcagcat gcagctctgt catagcttgt ttttctgcgg tgatgcgcgc tttcaccctg
     2701 cgaccagcaa tcctcggttg cgccgttttc tgcgatccac tccgtgatcc gcgccgaggc
     2761 aacgatgttg ccgtttgcgt gttagcgtcc acagccacac cgcttgcgcc acacaacagt
     2821 ggcgagtgcc gccgatctca ttccaaccgc aatctctctt gattctcatc gtgcggcggt
     2881 gatctacact ataaaacatc tagaaaggaa agcgatgaac acggcaatgg ccgctccaac
     2941 cgatgacccc acgcgcccgc atgagggccc acaaattgac cacgaaattg accaggaaat
     3001 ggaacatgcg gcacgtgctc gtctcaaaat gggctggggc cagagagcag tcgtcgcact
     3061 gctcttgact gtggtcatcg tcccgccgct cgcgggtttt tacagctact tctcgggcgt
     3121 accgctccat ttgcttgcgg ccaggcaaga cgacgaagac aaggaagccg ggggctcgac
     3181 cgcctccacc ggcatctcac tcgtagcggg ccaagcccat acggtcgagg tcccggacga
     3241 ggtcgccgcg accttgggaa tccgcaaagg cgataaggat tcggtcacca tcgcccaacc
     3301 gccgagcatg atgaagccgc tcgtgctccc cggttcaacg gcgctcgatc ccactcacct
     3361 tgcccgcatc cgcgctcgat tcgctccggc ccgagtcgtc gagatagccc aggtccagga
     3421 ccctttccgc aaagccggac gaacggaatt ccgcgagcta cgacaaggcg atagcgtgtc
     3481 gaagggcgat ttgctggggg ttttctacag tctggacgtg ggttccaaga agaacgatct
     3541 cctcgatgcc ctcgtgcaac tggaactgga ccagcacatt ctcgacaacg cccaagctgc
     3601 caggcaagcg gtgccggaag tctttatgct cactgccgag cgtgccgtgc agggagaccg
     3661 taacgccgtc aaccgggcca tcaacaacct caagctgtgg gagattcccc aggacgagat
     3721 cgatgcgctt catgccgagg cccagaagat ctgttccgac aagaccgcgt ggttcaagac
     3781 cccggagggc cggtgggtaa tgcgcgagaa acaagcccat ggcggcaagg ttgatcctca
     3841 caaggaagct gaaaacccct ggggccgggt gaccttgcgc gcgcctttcg acggcgttgt
     3901 cgtcgaacgc aacctcagcg tcgatgagat ggtcgtcgac aacacgatca acctgtttca
     3961 aatcgccgac gtgaaccggc ttctggttct cgccaactgc cccgaggatt cactgccgac
     4021 tttggaggct ttgagccaga accaaagacg gtggaccgtg caaacggtgg gcgcgaattc
     4081 ggccacggga cttcctggaa caatcgacga gatcggctac gtgatcgacc cgaatcagca
     4141 cacggcgatc atcaagggct atgtcgataa tccgggacgg catattcgtg cgggccaata
     4201 tgtcaccgcc acggtgcaga ttccaccgcc tgccggcgtg gtcgagattg ccgtcgatgc
     4261 cctggtcgac gatggcaggc agagtttggt gtttgtgcaa cccgatgcgg ataagcacca
     4321 ctttacgatg cggcgcgtcc acgtgacgca tcgcttcgac cactccgtgt tcgtgcgcag
     4381 cacgaattac tccaaggaag aacaactaac cgccctcgag gctgaagagg gcctcttgcc
     4441 caaagaaccc ctccggccgg gcgagcgcgt cctcgtggcc gggccggtcg agctcaaacg
     4501 agttgtgctc gacctcgagt cgcggcctaa agagaaaccg accgaccgcc tcgccaaggc
     4561 gaaaccgcga tcgtcgtcgt acttcgagtc gccaccgaag ccgaaagcca gtaagggcta
     4621 atcgactaga attcccaatg gcggattccc gatcccagac gagaggaaac gaggagttac
     4681 gtcgatgctt gcaccagttg ccacagattt actgctcata ggtatctttg tcatgatcgt
     4741 cggctgggtc tacggtgtgt tccggcgcca cgttttagtt gagcacactc aaccgccgac
     4801 atcggcacat gcggcggcct gctcgggccg gtttgtatac cgcgacaaat tctttcagca
     4861 acatgatttg cggctatgag catcctcttc gagtgcggcg gcgccgcctt gggaagaggc
     4921 tgatcctaga cctttggaag gttttcatgg ttcgcaagct gatcgcctgg gcgctcgata
     4981 atccgattgc cgtcatcctg gg
//
LOCUS       DATAQL010000992         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3457787, whole genome shotgun sequence.
ACCESSION   DATAQL010000992 DATAQL010000000
VERSION     DATAQL010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3457787"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VHS97_17155"
     CDS             <1..182
                     /locus_tag="VHS97_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449986.1"
                     /translation="GAYEDHDPYAALRSHHPDDVIGNSIFVFDLDRLGGGSPFRWPPP
                     KSQVGPGRVEEAKSK"
     gene            247..1833
                     /locus_tag="VHS97_17160"
     CDS             247..1833
                     /locus_tag="VHS97_17160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3449987.1"
                     /translation="MTTQRALGSVPDQTSRLAQHSASYTPWVCRAPLLVAAGLICLIS
                     AFNLARLVSAPAPRDPWEATEVIEAWRSLRGMPVYELKPDGHSTHVYGALVPWVQGEI
                     FRLVGPNNVSGRILSLVSGLAVVALLALTMRGEKTAWYLLVAGALILGSNNRSEQYFA
                     ENRPDMTALLFGAAGVLLIGLGQESRRWLLVAIGTACLVTGFFFKQTAFIFGAVPLAA
                     LVLRWRRPDRSEMILATLPLVVSVGVILALKIVNPTVYHYMIDVPKAFGLDWLRAVRC
                     FWDLLIDSPLFLVLLVECIAFDTKSIDRDPRLRWLFAVFAVAIPFSAITAGKVGGAPN
                     SLLPALLAMTAFCALRLPRFFRGLSDLQSPFPIRLLQGALLALLMLMSTFPHMTRSRG
                     WIALKNPWDHEYKKVVRLVEKLPGTVICPEDPTIPLYAKGYAGQNIFSEFDTHLVNGE
                     WPAVPPATFVASCRTADYVVDVTEHWEDLLEDDLLESLGFEPAPGIAPDMSCYRIWRR
                     KSLDPATISRTPRALPWASM"
     gene            2206..3117
                     /locus_tag="VHS97_17165"
     CDS             2206..3117
                     /locus_tag="VHS97_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449988.1"
                     /translation="MTTATHHDLVSDRIDARLRQGQLTEEQVDGFREFLNRVDRDLLH
                     HRIITDNAYSRWFSTGKATDAELVHFVRQFSVFSNQFLVAALLRVINAPTLEQARSAK
                     EILMNELGVIYRNTSGERAITRGGDDDVKDREGDPDLVSTEGTVDGGIFRFRAAHFEW
                     LLAVGETLGLGFDDLGKRRHGRPYVLRFCDELQRLFGAEDDQIAEGASFAVENWAAAG
                     FWQDLEDGLLQIKAARLPSLKVAFFTWHNRVEAQHAGHTMAELEQVFFAPNFDPARFF
                     QGAHEVLDAIAIFWDGLEDDRLRGVNG"
     gene            complement(3220..3411)
                     /locus_tag="VHS97_17170"
     CDS             complement(3220..3411)
                     /locus_tag="VHS97_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449989.1"
                     /translation="MKTVRWVLIACRILAVSIGMTLFNSGCGDQSGFSGGVSTEEEVK
                     RDAETRKAMEAASKVAKKK"
BASE COUNT          625 a         1085 c         1056 g          763 t
ORIGIN      
        1 ccggtgccta cgaggatcat gacccatacg cggcgctccg gtcccatcac cccgacgacg
       61 tcatcggcaa cagcatcttc gtcttcgacc ttgaccggct cgggggcggc tctccatttc
      121 gctggccccc tccgaaaagt caagtcggtc ctggccgtgt ggaggaagcc aaatcgaaat
      181 gacagacggc gttcagacag ccgggactga agtgccagac gccgcttttt tcaaggatga
      241 cttgccatga cgacgcagcg ggcccttggt tccgtacctg atcaaacctc acgattggcc
      301 cagcattccg cgtcttacac gccgtgggtc tgccgtgcgc cgttgctagt agcggccggg
      361 ctcatctgtt tgatcagtgc gttcaatctc gctcgtctgg tgagtgcgcc agcgcccaga
      421 gacccctggg aagcgacgga agtcatcgag gcctggcgca gtttgcgggg aatgccagtc
      481 tatgaactca agccggacgg acattcgacg catgtctatg gtgccctcgt cccctgggtg
      541 cagggagaga tctttcgctt ggtcgggccc aataatgttt caggccgaat cttatcgctc
      601 gtgtccggcc tcgcggtggt ggcgctcttg gctctgacga tgcgcggcga gaaaacggcc
      661 tggtatctcc tggtcgccgg ggccctgatc ctgggatcca ataatcgctc ggaacagtac
      721 tttgccgaga accggccgga catgaccgcg ttgctattcg gggctgcagg cgtacttctg
      781 atcgggcttg gccaggaatc gcgtcgatgg ctcttggtcg cgatcggaac agcctgcctg
      841 gtgaccggct ttttcttcaa acagaccgct tttattttcg gcgcggtccc cctggccgcc
      901 ttggtattgc gctggcggcg gccggaccga tcagaaatga tcctggccac ccttcccctg
      961 gtggtctcgg tcggcgtgat cctcgccctg aagatcgtga accccaccgt ctatcactac
     1021 atgatcgatg tccccaaggc ctttggactc gactggctgc gggccgtgcg gtgtttctgg
     1081 gacctcttga tcgattctcc cttgttcctc gtcttgttgg tcgagtgcat cgccttcgac
     1141 acaaagtcga ttgaccgcga cccgcggctg cgatggctct tcgccgtgtt cgcggttgcc
     1201 atccccttca gcgcgatcac cgcgggcaag gtcggaggcg cacccaacag cttgctgcca
     1261 gcactgctgg cgatgaccgc cttttgcgcc ctccggttgc cgcgtttctt ccgcggtctt
     1321 tcagatctcc agtcgccctt tcccattcga ctgctgcaag gagctctgtt ggcgcttctt
     1381 atgctaatgt ccacattccc acacatgacg agatccagag gttggatcgc actgaagaat
     1441 ccctgggatc acgaatataa aaaggtagtg aggctggtcg agaagctgcc gggaactgtc
     1501 atctgcccgg aagaccctac gataccgctc tatgccaagg gttatgcggg ccaaaacatc
     1561 ttctcggagt ttgacactca cttggtcaac ggcgaatggc cggctgttcc ccccgcgacg
     1621 ttcgtggcca gttgtcgcac cgcggattat gtcgtcgacg ttacagaaca ctgggaggat
     1681 ctactcgagg acgacctact cgaatcgctg ggattcgagc cggcgcctgg gatagccccg
     1741 gatatgtcat gctaccgcat ctggcggcgg aaatcgctcg atccggcaac gatctcgagg
     1801 acccccaggg cgttgccctg ggctagcatg tgaatgcccc ttcgggacgc gatcccggaa
     1861 cgacccccag ggcgttgccc tgggctaata tgtgaatacc ccttcggggc gcgatcccgg
     1921 aaggacccca gggcgttgcc ctgggctagc atgtgattgc cccttcgggg cgcgatcccg
     1981 gaatgacccc cagggcgttg ccctgggcta atatgtgatt gccccttcgg ggcgcgatcc
     2041 cggaagtagt aactttgcct ctttccccca gaggggcatt aacaaatcag cccagggcaa
     2101 cgccctgggt tttctcgcct cgaccgtgac cgcgcgcttc gattaaaata aaggtacatg
     2161 tcccccgcta ctcatcgtct actccaacaa cggagggggt gtttcatgac gacggcgacc
     2221 caccacgacc tggtttcgga ccgcatcgac gcacgtctgc ggcaaggtca actgaccgaa
     2281 gagcaggttg acggctttcg ggaattcctc aatcgagtcg accgcgactt gctccatcac
     2341 cgcattatca ccgacaacgc gtattcccgc tggtttagca cgggaaaggc gacggatgca
     2401 gagctggtgc attttgtccg ccagttttcg gtgttttcga atcagttcct ggtggccgcg
     2461 ttgctgcggg tgatcaatgc acctacgctc gagcaggcac ggtccgccaa ggaaatcctc
     2521 atgaacgagc tgggagtcat ctaccgcaac acgtccggcg aaagggcaat cacgcgcggt
     2581 ggcgacgacg atgtcaaaga ccgggagggg gaccctgatc ttgtcagcac cgaagggacc
     2641 gtcgacggcg gaatcttccg gttccgcgcc gcccatttcg agtggttgct cgccgtgggc
     2701 gagaccttgg ggctgggctt cgacgatctg ggcaaacgcc ggcatggacg gccgtatgtg
     2761 ctacgtttct gtgacgagct gcagcgcctt ttcggcgccg aagacgatca gattgctgaa
     2821 ggagccagct ttgccgtcga aaattgggca gccgccggtt tctggcaaga tctggaagac
     2881 ggtctgctgc aaatcaaggc cgcgcggctc ccctcgctga aagtcgcttt tttcacctgg
     2941 cacaaccgcg tcgaagctca gcatgccggc cacactatgg ccgagctaga gcaggtcttc
     3001 ttcgctccca acttcgaccc cgctcgattc ttccagggcg cccacgaagt tctcgacgcc
     3061 attgcgattt tctgggatgg gctggaagat gaccggctgc gtggtgtcaa cggttgaccc
     3121 tggctaatct attgcgccgc ggtggccacg gctcgggcca ccgcccgtag accctttcgc
     3181 cggtcggctc gagctggtcg tgtgggacgg ttggccgggt tactttttct tggcgacctt
     3241 ggacgcggcc tccatggcct tacgggtctc ggcgtctctt ttcacctcct cctcggtgct
     3301 cacgcctccg gagaatccgc tctgatcgcc gcaaccgctg ttaaagaggg tcatgcctat
     3361 agagacggcc aggatccggc atgcgatcaa aacccagcgg acggtcttca tgagagaatt
     3421 cccggttcga tgaggtctct gcgtacgagt ttgtacgggt cgatacggcg gcgactcgga
     3481 gacagcgccc aggccgccgg cggggcgggt agggcggcgc tcagtaagc
//
LOCUS       DATAQL010000993         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_405306, whole genome shotgun sequence.
ACCESSION   DATAQL010000993 DATAQL010000000
VERSION     DATAQL010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405306"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..625
                     /locus_tag="VHS97_17175"
     CDS             131..625
                     /locus_tag="VHS97_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449990.1"
                     /translation="MYGASKSTHRVDDVVSGRITPTWRLEAPLVAAAVSPLNWDIVDA
                     MSTVKELMLSPEGTVAEPLEAGLDEVPALAEVDAGAVVVDEDDEQPAATMAATAATAT
                     KPNRGTSLDRPRPSELEGRPPRALLSSSIPYPFRHNALGYATARDTAPHVCPSGMKGR
                     SWER"
     gene            1195..>1534
                     /locus_tag="VHS97_17180"
     CDS             1195..>1534
                     /locus_tag="VHS97_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3449991.1"
                     /translation="MVATKVLIIEDDPNIVDLIRSNLAVRGFDTVVSAGSKVIDLFDL
                     EQPDIVLLDLMLPEADGFELCREIRERSSVGIIVVSARGGEQDKVTALNMGADDYMTK
                     PFGIEELLARI"
BASE COUNT          305 a          470 c          479 g          280 t
ORIGIN      
        1 cacgatcagg accttggcac ccaggttgat gtcggcggtc gcgtcgctca gctgcgtcgc
       61 gtcgattccc tgcgcgttgt cgatgcggaa ctccgacgag gtgtacccgg cggcggtgaa
      121 ggcttccttc atgtacggcg cgtcgaagtc aacccaccgg gtcgacgacg tggtgtcggg
      181 caggatcacg cccacctgga ggctagaggc acccttggtc gcggccgccg tcagcccctt
      241 gaactgggac atcgtcgacg cgatgtcaac cgtgaaggag ctgatgctca gccccgaggg
      301 aaccgtggcc gagccgctcg aggccgggct ggacgaggtc cccgcgctcg ccgaggtcga
      361 cgcgggcgcc gtggtcgtcg acgaggacga cgaacagccc gcggccacca tggcagcgac
      421 cgctgccacc gccacaaagc ccaaccgagg caccagcctc gatcggccgc ggccttctga
      481 actggagggc cgccctcccc gtgctctttt atcaagtagc atcccatacc cctttcgcca
      541 caatgcgctt ggatacgcca ccgcgcggga caccgcacca cacgtgtgtc cttccgggat
      601 gaaaggtaga tcttgggaac gctagacttc aagggccgaa acccacccgt gatctaattg
      661 gactagaccc gtcgatcttg gctctcgcaa gagcgacggg gccacgaaaa atagcgccag
      721 attgcgcact ggtcaatatg tgcgccatcg ccgatgcgtt aatattttat caataatttt
      781 tggttgctag ttgagtacca aatcagtccg ctttgttgga gtcacgtaac gatcgagtct
      841 ggctcctagg tgacaagcgt acccccctcg cccgtcactg ggcagtggcg cagcgtatgc
      901 agtgcgatgc cgagtccgcc aactgcgagg cgggctccag tcgagacgca gttgcgctgg
      961 tagacccgct ggacgaggtc ggacttagcg aggcggaggc ccttctcccc cgttccggaa
     1021 gctcgcgaaa caaacacgaa aatcagcgga tcggcacggc gacgcattga gcgctacgca
     1081 acttcgatct accttttgag gatgctggct catggcgggc aaaccgtctt atagttgcgc
     1141 gatacgggga tgatgaatca ctggagaaag cctatgacta cggcgagtaa tccgatggtc
     1201 gcaacaaagg tgttgattat cgaagacgat ccgaacatcg tggacctgat tcgctccaac
     1261 ctggccgtgc ggggcttcga cacggttgtc tcggccggaa gcaaggtgat cgacctgttc
     1321 gacctcgagc agccggacat cgtgctgctc gacctcatgc tgcccgaggc cgacgggttc
     1381 gagctgtgcc gcgagatccg tgagcggtca tccgtcggca tcatcgtggt ctcggcgagg
     1441 ggcggggagc aagacaaagt cacggcgctg aacatgggcg ccgacgacta catgaccaag
     1501 ccgttcggca tcgaggagct gctggcgcgg atcc
//
LOCUS       DATAQL010000994         5118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_405339, whole genome shotgun sequence.
ACCESSION   DATAQL010000994 DATAQL010000000
VERSION     DATAQL010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405339"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2014
                     /locus_tag="VHS97_17185"
     CDS             <1..2014
                     /locus_tag="VHS97_17185"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HEX3449992.1"
                     /translation="WLVYLIILGAYLSLRGYHSFDSDQAYRLPLLLHRQDPTMFAADP
                     FVRAFDVFNPHRGTLWVLDLVTRPLGLSAGLLLVFVLTFFATCRGVDRLTQAVWPEAG
                     GQVGVFAIGLVLAAKAGNIGTNHLFEAMVLDRLMAFALGWLALAQVIVEPSRRRLGAS
                     AAVAAATVIHPSVGLQLALVLAASWTVWWLLGRWMKVDRFDAFLGIAGLGIAVLPGVA
                     LNLAGGSSPQGNMPADAFWLLSVELQSPQHMLPHLWRMPQWLAAGCYVVLAALALFGQ
                     YRWSQADPAEPSNEMIGRTPWTPARLRLIVMLAVILAGLGTAWYAVEVLHDVRITIFQ
                     PFRMATIARGIALVFVAGRLAALWRLGGWPARLRAILLAVAFTGDWLLVVVTLVELSV
                     AASGAVRALCPWWRDYRKIDLLAMSAMLALGLNFLVHHDTEYGHIPLLAALGLGLLTG
                     LWGRLRINARTHGENWVWTPARVRAATALAWGLPLASLLAAAVPTDHALARHPLVRGL
                     IARARFAEVPADDVERLGRWCRHHTPPTSRFVGPPGPKTFRLWSRRSLAFNRAASPYH
                     ADGLADWFARFQDHVDFHGIPADFAHAYVSDRHGFEARYQSQSDSERAALALRQGATY
                     VVAAAPSMRPQDSRFRPSAGPLELLHVEGRYAVYRVKPELVVQRQR"
     gene            complement(1972..2628)
                     /locus_tag="VHS97_17190"
     CDS             complement(1972..2628)
                     /locus_tag="VHS97_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3449993.1"
                     /translation="MTSLPDNPSSAFFWAEAPDRTDMCGLGMRVAFTRVGPLWAHSLF
                     VSRADGLALVQTVDNDPEHGDPARVVSPVYQEIRRHECAQDAGRCLLLTGTYFQHHFS
                     AAVTLGIDPERPVSPVLDVDVADRCRVRVAAFAATYTVQLDSGALKDASPQAIIWDVL
                     GPDIGRLELVAIAPGTLALAEAGRNATRVQVVAAIESGSFTHRLRYRWRWTTNSGLTR
                     "
     gene            complement(2625..3750)
                     /gene="prfB"
                     /locus_tag="VHS97_17195"
     CDS             complement(join(2625..3680,3682..3750))
                     /gene="prfB"
                     /locus_tag="VHS97_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244732.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX3449994.1"
                     /translation="MDAELKRNAKAVIDKILHLRDSLDFGEKQSRRDALEQEMNSATF
                     WNDQEKAKAVIQDLKSMNTVLKPFEALVRQAENLDATIELAEEAETDEFDDEIRAACK
                     QADTDFGEFEFRSMLSGPNDHCNAFVTIHAGAGGTEACDWAEMLLRMYLMWAESKRFP
                     TQITDREDGGAAGIQEATIHIKGEYAYGYLRGETGVHRLVRISPFDSNARRHTSFASI
                     DVLPEVDDTIDIVLRDEDLKRDVFRSGGPGGQHQNKTESGVRYTHLPTGVAAESRSER
                     SQHKNDTNALATLKAKLIRLEEEKREAEFAKKYDEKGEVSFGNQIRSYVLQPYQIVKD
                     LRTDHEVGNPRAVLDGDIDGFIEAYLRMKLAKGGTDLAKA"
     gene            3908..4903
                     /gene="gluQRS"
                     /locus_tag="VHS97_17200"
     CDS             3908..4903
                     /gene="gluQRS"
                     /locus_tag="VHS97_17200"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX3449995.1"
                     /translation="MTEGAYPGGTNRKPGGRLAPSPTGGLHLGHARTFLIAWLAARHS
                     GVRVILRIEDLDSSRVRAQSIATALADLHWLGLDWDEGPDVGGPSAPYTQSERLGLYE
                     EALDLLIARELVYPCTCTRADIERAALAPHAEDEGPTYPGTCAHRNVNDARALGDRTF
                     AWRFRVPAERVSWNDLYLGRVELDPSQVGGDFVVARQTVGYSYQLAVVTDDSAMGISQ
                     VIRGMDLVPSTPRQILLYQALGLPVPAFGHVALAVGPDGRRLAKRDGSLKLATLREAG
                     VDPCRLVGMLIHSCGWSDRAVAITPRAAIDRYAPTRLAALPWVVSEPWLESLQRR"
BASE COUNT          799 a         1557 c         1653 g         1109 t
ORIGIN      
        1 gtggctagtt tatttgatca tcctgggtgc gtacctcagt ctacgcggct accacagctt
       61 cgatagcgac caggcctacc gcctgcccct actgcttcat cgtcaggatc cgacgatgtt
      121 tgccgccgat ccgtttgtga gggcattcga cgtcttcaac ccgcatcgcg gtacgctctg
      181 ggtgctcgac ttggtcacga gaccgctcgg gctctcggcg gggctcttgc tcgttttcgt
      241 tctgaccttt ttcgcgactt gccgcggtgt cgatcggctg acgcaggcgg tttggcccga
      301 ggcgggcggt caggtcggtg tgtttgcgat cggattggtc ctggcggcca aggccggcaa
      361 catcggcacc aatcaccttt tcgaggccat ggtactggat cggctaatgg cctttgcgct
      421 cggctggctg gccctggcgc aggtcattgt cgagccgagc cggaggcgct tgggcgccag
      481 tgctgccgtc gcagcggcga ccgtgattca tccctcggtc ggactccagc tcgcacttgt
      541 actagcggca agctggacgg tctggtggct gctcggccgt tggatgaaag tcgaccggtt
      601 cgatgcgttt ctcggaatag caggcctggg gatcgccgta ctaccgggcg tggcgctcaa
      661 cctggcggga ggtagttcgc cacaagggaa tatgccggcg gacgcctttt ggctgctgag
      721 tgttgagcta caaagcccgc agcacatgct gccgcatctg tggcggatgc cccagtggct
      781 cgcggccggg tgctacgtcg tgctggccgc gctggcgctt tttggtcagt accggtggtc
      841 acaagcggat ccggccgaac cgtcgaatga aatgatcggg cggacgcctt ggacacccgc
      901 ccgccttcgt ctaatcgtaa tgctggcggt cattctcgcc ggactgggaa cggcctggta
      961 cgccgtcgag gttcttcatg acgtgcggat cacgatcttc cagccgttcc gtatggccac
     1021 catagcccga ggcattgccc tggttttcgt cgccggccgg ctcgcggccc tctggcgttt
     1081 gggcggatgg ccggcgcgac tgcgggccat cctgctcgcg gtggcattta ccggcgactg
     1141 gctgttggtc gtggtgacgt tggtggagct atccgtcgct gcgtctggag cggtgcgggc
     1201 tctttgccca tggtggcgag actatcgaaa gatcgatctc ctggcgatgt cggcaatgct
     1261 tgccctgggt ttaaacttcc tggttcatca cgacacggaa tatgggcaca ttccgctttt
     1321 ggccgccctt gggttgggtt tgctcacagg attatgggga aggctgcgga taaacgctcg
     1381 aacccatggc gagaactggg tctggacgcc tgcccgcgtg cgggcggcga cggcgctcgc
     1441 atggggtctg ccgctggctt cgctgctggc agcggcggtt ccaactgacc atgctctggc
     1501 gcgccatcct ctggtgcggg ggttgatcgc gcgcgctcgg tttgcggagg tgccggcgga
     1561 cgacgtcgag cggctgggcc ggtggtgccg tcatcacact ccgcccacgt cgcggttcgt
     1621 cggaccgccc ggtcccaaga cgttccggct gtggtcgcgg cggagtctgg ccttcaatcg
     1681 tgcggccagc ccctatcacg ccgacgggct ggccgactgg ttcgcgcggt ttcaagacca
     1741 cgtcgatttc cacggaatcc ccgctgattt tgcgcatgcg tacgtgagcg accgccacgg
     1801 attcgaggca cggtatcagt cccaaagcga ctccgagcgg gcggccctgg ccttacgtca
     1861 gggggcaacg tacgtcgtcg cggcggctcc gagcatgcgg ccgcaggact ctcggtttcg
     1921 accgtcagcc ggccccctgg agctacttca cgttgagggc cgttatgccg tctaccgggt
     1981 caagcccgag ttggtggtcc agcgccagcg gtagcggagc cgatgtgtaa agctccccga
     2041 ctcaatggcg gccacgactt ggacgcgcgt cgcatttcgg ccggcttcag caagggcgag
     2101 tgtaccgggt gcaatggcca cgagctcgag ccggcctatg tctggtccca acacgtccca
     2161 gatgatggcc tgcgggctgg cgtctttgag cgcgccgcta tcgagttgga ccgtgtaggt
     2221 cgcggcgaaa gcggcgacac gcacacgaca gcggtccgcg acatccacgt cgagcaccgg
     2281 agaaacgggc cgctcaggat cgattcctag ggtgacggcc gccgagaaat ggtgctgaaa
     2341 gtaggtgccg gttaacagta ggcagcggcc ggcatcctgg gcgcattcgt gccggcggat
     2401 ctcttgatag acggggctga cgactcgggc gggatcgccg tgttccggat cgttgtccac
     2461 agtttgcaca agcgcgaggc catcagcacg ggaaacgaag agggagtgag cccaaagtgg
     2521 accgacccgc gtgaacgcca cgcgcatgcc cagaccgcac atgtcggtgc ggtcgggcgc
     2581 ttccgcccaa aagaaagcag atgatggatt gtcgggcaag ctcgtcacgc cttggcgagg
     2641 tccgtgcccc ctttggcgag cttcatacgg aggtaggcct cgatgaagcc gtcgatgtcg
     2701 ccatcaagga cggcccgcgg gttacctact tcgtggtcgg tccgcagatc cttgacgatc
     2761 tggtagggct ggagcacgta cgagcggatc tggttgccga agctgacctc ccctttttcg
     2821 tcgtacttct tggcgaactc ggcctcgcgt ttctcttcct cgaggcgaat gagcttggcc
     2881 ttcaaggtgg ccagggcatt ggtgtcgttt ttgtgttgcg agcgttcgct gcgcgactca
     2941 gcggcgacac cggtcggaag gtgagtgtat cgtacacccg attcggtttt gttctgatgc
     3001 tggccaccgg gtccgccgga gcggaagaca tctcgcttca agtcttcgtc gcggagcacg
     3061 atgtcgatcg tgtcgtcgac ttccgggagc acatcgatcg aggcgaacga ggtgtgccgc
     3121 cgtgcgttcg agtcaaacgg gctgatccga accagccggt gaacgcccgt ttcgccgcgg
     3181 agatagccgt aggcgtactc tcccttgatg tggatagtgg cctcctggat gccggccgcc
     3241 ccgccgtctt cacggtcggt gatctgggtg gggaatcgct tcgactcggc ccacatgaga
     3301 tacatgcgca atagcatctc cgcccagtcg caggcctccg ttccccccgc gccggcgtgg
     3361 atcgtcacga aggcgttgca gtgatcgttg ggtccgctta gcatcgagcg gaactcgaat
     3421 tcgccgaaat cggtgtcggc ctgcttgcat gccgcgcgga tttcgtcgtc gaactcgtcg
     3481 gtctcggcct cctcggccag ctcgatcgtc gcgtcgagat tctcggcttg cctgacgagt
     3541 gcctcgaaag gcttcaagac cgtgttcatg ctcttgaggt cctggatgac cgctttggct
     3601 ttctcctggt cgttccagaa cgtcgcgctg ttcatttctt gctcgagcgc gtcgcggcga
     3661 gattgttttt cgccgaagtc aaagagagtc tcgcagatgg aggatcttgt cgatgacagc
     3721 tttggcgttt cgtttcagtt cggcatccac ggtgcggcac tccttcgtct cggcgaattc
     3781 ttgcagattc tggtcttcaa tcgtgacgac cagatcaatc gattacctct accattagat
     3841 ccgatcgagc gattttgggc cagtcgaacc tttgccgaag gcccctttcc tggtattcgg
     3901 tgtggagatg acagaaggcg cgtaccctgg cggaactaac agaaagcctg gcggccggct
     3961 cgctccctcg ccgaccgggg gcctccatct ggggcacgcg cggacatttc tcatcgcctg
     4021 gctggccgca cggcatagtg gtgtgcgagt catcctacga attgaggatc tggattcctc
     4081 gcgggttcgc gcccaatcca ttgccacagc tcttgccgac ctccactggc tggggctcga
     4141 ctgggacgaa gggcccgacg tcggcggacc gtctgcccct tacactcaat ctgagcgttt
     4201 ggggctttac gaggaagcgc tcgatcttct cattgcccgt gagctcgttt atccctgtac
     4261 gtgcacgcgg gccgacatcg agcgtgcggc cttggcgcct cacgccgagg acgaggggcc
     4321 gacctacccc gggacgtgtg cacacagaaa cgtgaatgat gctcgtgccc tgggcgaccg
     4381 tacgttcgcc tggcggttcc gagtccccgc cgaacgtgtt tcctggaacg atctctacct
     4441 tggccgcgtc gagctcgacc catcccaggt cggtggcgat ttcgtcgtag ctcgacagac
     4501 cgtcggttat tcatatcagc tcgcggtggt gaccgacgac tccgcgatgg gaataagcca
     4561 ggtgatccgt ggcatggatc tggtgcccag tacgccgcgg caaatcttgc tctaccaggc
     4621 gctcggcttg ccagtgccgg catttggcca tgtcgcactg gctgtcggcc cagacggacg
     4681 ccggctcgcc aaacgtgatg gctcgctcaa gctcgcgacg ctacgcgaag ccggagtcga
     4741 tccgtgccgt ctggtcggca tgttgatcca ctcgtgtggt tggtcggata gggcggtcgc
     4801 gatcacgccg cgcgcggcga ttgaccgtta tgcgccgact cgacttgccg cgttaccgtg
     4861 ggtcgtctca gagccatggc tcgaatcgct tcagcgacgt tgataaaaat tccaactccg
     4921 cagcgacctc ggcccgagtt cgcgtcaatc aaacttcacg acggtacgct ctcccgcata
     4981 ttgcgtcgtt cgagcggcat tggagggcac gagtctgact tcgaatcgcc tactcgcgtc
     5041 gggctttttc gtcgagcgtt tatccggctc aatggtcaag gccctggcct tgtcgtccca
     5101 tcggattcga gtccacgt
//
LOCUS       DATAQL010000995         5538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4373580, whole genome shotgun sequence.
ACCESSION   DATAQL010000995 DATAQL010000000
VERSION     DATAQL010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4373580"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VHS97_17205"
     CDS             <1..1131
                     /locus_tag="VHS97_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX3449996.1"
                     /translation="VRSGDRDRGQEGRPGARTGKVIGVRGKSVFVDLGGKSEGVIAVS
                     QFEEGKLPLPGATIEVIVDRFDPDEGILLLRLKGSAIEADWDNLKKGVIVEARATKTV
                     KGGLEVDVDGIRGFLPISQIDLSRIDDAASYVNQKFKAVVTEANQREKNLVISRRELL
                     EQERAEQRERTWATLEEGQVRDGVVRSIKDFGAFVDLGGVDGLLPIGEMSWSRLSKVD
                     EMVKVGDQVKVKVIKIDKTARKLTLGLKQLTPSPWEAAAEKYPRGMMVKGKVSKIMDF
                     GAFVELEPGIEGLIHISELSPSRVRRVVDIVKPAQEVEVRILKVEPEVKRIALSLLPA
                     KGTPVVEEEDDTDDDETPPPPEPEPKIPLKGGLGDRERRNNR"
     gene            1348..1740
                     /locus_tag="VHS97_17210"
     CDS             1348..1740
                     /locus_tag="VHS97_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3449997.1"
                     /translation="MSRFLKVMPRLPVADLQRVIAFYKNVLGFRAGSLWPEDEPCFVL
                     LDRDDVCVQFYVADRSKGEAVGYGTLSYEVDDAMATYAALEGLAPIEWGPEVYWYGRR
                     EFAIRDPSGYLIIFSEPTTDPPTCVNEG"
     gene            1747..2535
                     /locus_tag="VHS97_17215"
     CDS             1747..2535
                     /locus_tag="VHS97_17215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3449998.1"
                     /translation="MPELSSLNPTGRFTGLAETYARFRPSYPAHGIDFIMARCGLEKA
                     SLLVDVGCGTGIASRLFAARGINVIGIEPNAEMRCAAAAEANADAKKPSLTYREGRAE
                     STGLEAAVADAVLSAQAFHWFEPVATLREFHRILKPVGWTILMWNERDERDPVTAAYG
                     AVIRTADDAGAIEGSRQTRAGEVLRANPLFRQAEQVKFYNQQALDEEGLLGRAFSVSY
                     APREASERDAWADRLKAVFRQYQWNGKVELRYQTTIYVAQRNGG"
     gene            complement(2611..5268)
                     /locus_tag="VHS97_17220"
     CDS             complement(2611..5268)
                     /locus_tag="VHS97_17220"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3449999.1"
                     /translation="MRWGLGPVFLYECLANSRRWQTYAIRSVGVAVMLAAIATIAMSH
                     TAADSESGWRRYAALGASYFAAIIGVELTLVLLAAPAATAGAICVDRARGTLTHMLAT
                     DLSDPEIVLGKLAARLLPVFGLVACSWPVMAISSLLGGIDPIALTLAFAIILAVAVLG
                     CTMALALSVWARKTHEVILATYTVFILGVLFWPIWYFLSNAGPVSAPPPWALLPNPFY
                     VAFAPYADPGKLDFWDYFVFFGAALLASASLTTLAVWRMRPVASRGSVERNMGPRMGP
                     VGRMTRWLPGPSLDRNPVLWREWHRTRPSRWMTAILALLMGTTGVLCLVGAIAFWKNG
                     VDFRPAAIWNVAGECSYVLHVTFGLLMLAAIAPTSMAEERQRGSLDVLAATALSTRSI
                     VIGKWLGTFRLVLLMAVGPGLMALAMATAHAENVTIFRPVLSPDVYRVPTLGARCYGV
                     IVVIATIFAHGALITSVGLALAVWIKRQSRAIAMSVGSFILVTAAWPMVVTTAINGGS
                     HFGRDLASLSPVVACVIFDNYFTFRRNAQVGGIPSSGTFLAVEVFAPAMGLLWLTVRT
                     FDGCFDRIPDRPQRIAVRTIAVMILAAMIGAGSLVAAIDIWIEGFKPGSTSRPMPLGI
                     VGYSLLIAIGLMLVALESAKSVRPAWITFPAEAPGLAARGFVLGQWWKSFRLVLLLAI
                     GPALLALALATAHKAPSYVPQFTTNPAGVKVVTSYVLARAGTPYFGEVRLGQRLMIAA
                     LLIVTVLVHGGAAISVGLGFATTKVWSRRALAAAVGLIVLVVFVLPLCLIMVNNGRAL
                     DAVMLCFVMASDSLLTVLVTRVSFSVADMLWSVMFCDVVIALFAIGVSWWTMRVWKCR
                     LSDVPKAKPSLATDLHDGRPAAEAVLIGD"
BASE COUNT         1187 a         1683 c         1775 g          893 t
ORIGIN      
        1 gtgcgctcgg gcgatcgcga tcggggccag gagggccggc caggcgcacg cacgggcaag
       61 gtgatcggcg tgcgcggcaa gagcgtcttc gtcgatctcg ggggcaagag cgagggtgtc
      121 atcgccgttt cgcaatttga agaaggcaag ttgccgcttc ccggcgcgac catcgaagtg
      181 atcgtcgacc gcttcgatcc cgacgaaggg attctgctgc tccgtctcaa gggctcggcg
      241 atcgaagccg attgggacaa cctcaagaaa ggcgtgatcg tcgaggcgcg ggcaaccaag
      301 acagtcaagg ggggcctcga ggttgacgtc gacggcattc gcggcttcct ccccattagc
      361 cagatcgacc tgtcgcggat cgatgacgcc gcatcatatg tcaaccagaa gttcaaggct
      421 gtggtcaccg aggccaatca acgcgagaaa aacctggtga tctcgcgccg cgagctactg
      481 gaacaagaac gtgccgagca gcgtgagcgc acctgggcca cgctcgaaga gggacaagtg
      541 cgcgatggtg ttgtccggtc gatcaaagac ttcggtgcgt tcgtcgacct ggggggtgtc
      601 gacggtctgc tgccgatcgg cgagatgagc tggtcacgtc tctccaaggt cgacgaaatg
      661 gtcaaggtcg gcgaccaggt caaggtcaag gtgatcaaga tcgacaagac ggcgcgcaag
      721 ttgaccctcg gcttgaaaca gctcaccccc agtccctggg aagccgccgc ggaaaagtat
      781 ccccgcggca tgatggtgaa gggaaaggtc tccaagatca tggacttcgg cgcgttcgtc
      841 gagctagagc ccgggatcga gggcttgata cacatttcgg aactgtcgcc cagccgagtg
      901 cgccgggtcg tcgatatcgt caagcccgct caggaagttg aggtgcggat cctcaaagtc
      961 gagcccgaag tcaagaggat tgcactttcc ctgttgccgg ccaaggggac gcccgtggtc
     1021 gaggaggaag acgacacgga cgacgacgaa acgccgccgc ctcccgagcc cgagcccaag
     1081 atccccttga aaggcggtct cggagatcgc gagcgacgaa acaatcgcta acttggatcg
     1141 cgggcagatc gacgaatgac tctccaggta cttttagtaa ccgcgaaaga ataagttgac
     1201 caagcgggct aggcgggctt atgtggcacg ggcatcttgc ccgtggtatt tttagacgca
     1261 ggctagaagc ccgtgccacg acgggggccg aaattcgctc aacctattat ttcacggcgc
     1321 cgctccggcc ggagcagcga ggaatccgtg tcgcgcttct tgaaagtcat gccgcggctg
     1381 cccgttgccg atttgcagcg cgtgatcgcg ttttataaga acgtgctcgg tttcagggcc
     1441 ggttcgctat ggcccgagga tgagccgtgt ttcgttctgc tcgacagaga cgacgtgtgt
     1501 gttcagttct acgtggccga tcgatcgaag ggtgaggctg tcggatacgg cacgctttcg
     1561 tacgaagtcg acgatgcgat ggccacttac gccgcgctcg aaggcctcgc accaatcgag
     1621 tggggaccgg aagtttattg gtacggccgt cgtgagttcg cgatccgaga tccgagcgga
     1681 tatctcatca tcttttccga accaacgaca gatccgccaa cgtgtgtaaa tgagggttga
     1741 gtactcatgc ctgaactgtc gagcctcaat cccaccgggc gttttacagg gcttgccgag
     1801 acatatgcca gattccggcc gagctatcct gcccacggca tcgacttcat catggctcgt
     1861 tgcgggttgg aaaaggcgtc tctcctggtc gacgtgggct gcggcaccgg tattgcctct
     1921 cggctgttcg cggcgcgcgg tattaacgtg attggcatcg agcccaatgc ggagatgcgc
     1981 tgcgccgccg cggcggaagc gaacgccgac gcaaagaagc ctagtttgac ctatcgtgaa
     2041 ggtcgcgcgg aatccactgg tctagaggcg gcggttgccg acgcggttct gtcggcacag
     2101 gcctttcact ggttcgagcc ggtcgcgact ctccgcgagt tccaccgaat cttgaagccg
     2161 gttggctgga caattctgat gtggaacgaa cgcgatgaac gagatcctgt gaccgccgct
     2221 tacggagcgg tcattcgcac cgcggacgac gccggggcca ttgagggttc ccgtcagacg
     2281 cgtgccggcg aagtcttgcg ggccaacccg ctcttccgcc aggccgagca ggtcaagttt
     2341 tacaaccagc aagcactcga cgaggaaggg ctcttgggtc gggctttctc ggtctcgtac
     2401 gcacctcgcg aggccagcga gagggatgct tgggccgatc gcctcaaggc ggtatttcgt
     2461 cagtaccagt ggaatggaaa ggttgagctc cgctaccaga cgacgatcta tgtggctcaa
     2521 cgaaatggcg ggtgaatggg acaaagccgg gtggtcggga tgagtgacgt tcgatcagga
     2581 ctcgcatgtc agaccgcgca gcaggaccga tcaatcgccg atgaggacgg cctcggcggc
     2641 aggacgaccg tcgtgaaggt cggtcgcaag cgaaggcttg gctttgggta cgtcactcaa
     2701 tcggcatttc cacacccgca tcgtccacca cgaaaccccg atggcgaaaa gtgcaatgac
     2761 aacgtcgcag aacatcacgg accagagcat atctgcgacg ctgaacgaca cacgggtgac
     2821 gagcaccgtg aggagagaat ccgacgccat gacaaagcac aacatgacgg catccagagc
     2881 acgaccgtta ttgaccatga tcaggcagag cggcagaacg aaaaccacca gcacgatgag
     2941 tccgacagcg gccgcgaggg cacgtcttga ccacactttc gtggtggcga atccgaggcc
     3001 aacgctgatc gccgcgccgc catggacgag gactgtgacg atcaaaaggg cggcgatcat
     3061 caaccgctga ccaagtcgga cctcgccgaa atacggggtg cccgctctgg ccaggacgta
     3121 tgaggtaacg accttcacac ccgcagggtt cgttgtgaac tgcggtacgt aactcggggc
     3181 tttgtgcgcc gtggccaggg ccaacgcgag gagcgccggg ccgattgcca acagcaagac
     3241 caggcggaat gacttccacc actgacccag gacgaagccc cgggcagcga gccccggggc
     3301 ctccgcggga aaagtaatcc acgccgggcg taccgacttc gccgactcca gcgcgacgag
     3361 catcaacccg atcgcgatca ggagagaata gcccacgatt ccaagaggca tcggcctgct
     3421 ggtcgacccc ggcttgaacc cttcgatcca aatgtcgatt gccgcgacta acgaaccagc
     3481 gccaatcatt gcagccaaga tcatgaccgc gatcgtccgt actgcgattc gctgaggccg
     3541 gtcagggatt ctgtcaaagc agccgtcaaa cgtccggacg gtcagccaca agagccccat
     3601 cgcgggggca aagacttcga cggccaagaa cgtgccggac gagggaatgc ctccgacttg
     3661 tgcatttctg cgaaacgtga agtaattgtc gaaaatgacg caagccacaa ctgggctaag
     3721 ggaagcaagg tctcggccga aatgggaacc cccgttgatg gcggtggtca cgaccatcgg
     3781 ccaggccgcg gtgaccagaa tgaacgagcc gacgctcatg gcgatcgccc ggctctgacg
     3841 cttgatccag acggcgaggg ccaggccgac gctggtgatc aaagcgccgt gcgcaaagat
     3901 ggtcgcgatg accacgatga cgccatagca ccgggcgccg agcgtgggca ctcggtagac
     3961 atcgggcgac agaacaggcc ggaatatagt gacattctcc gcatgcgccg tggccatggc
     4021 gagcgccatc aatcccgggc cgaccgccat cagtaagacc agacgaaacg tccccagcca
     4081 tttgcctatc acgatcgacc gcgtggagag tgccgtggcg gccaagacgt ccaggctgcc
     4141 ccgctgccgc tcctcggcca tggaagtcgg ggcgatggcg gccagcatca gcaaaccgaa
     4201 ggtcacgtgg agaacgtagg aacactcgcc ggccacgttc cagatcgctg ccggtcgaaa
     4261 atccacaccg tttttccaga acgcgatggc accgaccaag caaagaaccc cggtcgttcc
     4321 catgaggagg gcgaggatgg ccgtcatcca gcgggaaggc cgcgtacgat gccactcgcg
     4381 ccacagcacc ggattgcggt caagcgacgg accgggtagc catcgcgtca tccggccaac
     4441 agggcccatt cgcggtccca tgttcctctc gaccgaaccg cggctggcga cgggccgcat
     4501 ccgccagacc gcgagtgtgg tcagcgatgc ggacgccaga agcgcggcgc cgaagaatac
     4561 gaaatagtcc caaaagtcga gcttgcccgg gtcggcgtag ggagcgaacg ccacatagaa
     4621 cgggttgggc aggagggccc acggcggagg tgcgctgacc ggccctgcgt tcgataagaa
     4681 gtaccagatc ggccagaaaa gtacgcccag aatgaaaacg gtataggtcg ccagaattac
     4741 ctcgtgcgtc ttcctggccc agaccgatag cgcgagggcc atcgtgcagc cgaggacggc
     4801 cacggccaag atgattgcga acgccagagt cagcgcgatc gggtcaatcc cgcccagcag
     4861 cgaactgatc gccatgacgg gccaactaca agcgaccagg ccgaataccg gtaagagccg
     4921 cgcggccagc ttgcccagca cgatctcggg atccgagagg tcggtggcca gcatgtgcgt
     4981 gagcgtcccg cgggcgcggt ccacgcagat ggccccggcc gtcgccgccg gcgcggccag
     5041 cagcacgagg gtcagctcca cgccgatgat cgcggcgaaa tacgacgccc ccagtgcggc
     5101 gtagcgccgc caccccgact ccgagtcggc cgccgtgtgc gacatcgcga tcgtcgcgat
     5161 cgccgcgagc atcaccgcga cgccgaccga acggatggca taggtttgcc atcgccgcga
     5221 gttggccagg cactcgtaga ggaagaccgg tcccagcccc cagcgcatca caccggccct
     5281 ccatcgcggg gatgacccgg caggacggga atcccggatt ccggatccgg agatccgacg
     5341 cggcctcatt ccgagcccgg gatccggccg cctggtcgcc cgacgccatc catcgcccat
     5401 cgtagcgccg cgaggcggcg ggggcaattg cctgctcgga cattcgtcgg ggcagggggg
     5461 tgaaactcac cggatgttcg aggtgacgcg gcctcagatc gacgcgccca ccgactcgcc
     5521 cacggccggc tgacgcaa
//
LOCUS       DATAQL010000996         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4985451, whole genome shotgun sequence.
ACCESSION   DATAQL010000996 DATAQL010000000
VERSION     DATAQL010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4985451"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VHS97_17225"
     CDS             <1..536
                     /locus_tag="VHS97_17225"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3450000.1"
                     /translation="DVYPIDTARLRRVVELVADKAEWGKKLPPRHGQGIAVHRSFVSY
                     VATVVEVAVDDKGRLSVPRVDTAIDCGFAANPERIYSQVEGAAVMGLSLAKYGEISFK
                     NGRVEQNNFDGFQVLRIDESPPITHVWIVPNGIDVPSSGVGEPGLPPFAPALCNAIFA
                     ATGKRIRRLPIADQLAI"
     gene            681..>1722
                     /locus_tag="VHS97_17230"
     CDS             681..>1722
                     /locus_tag="VHS97_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3450001.1"
                     /translation="MRTRQSMWTGLATASALWSAIAAAGEVGNYSPVTADRLTNPEPA
                     NWMLYRRTYDGQGYSPLDKINTGNVKNLVPAWTFSTGVNEGHEAPPVVNNGIMFVATP
                     QNQVIALDAKTGDQIWRYKRELPEDLSQLHPTSRGVGLWQDKLYLATTDDHLVALDAK
                     TGKVAWDQKVQDYKKGQYLTLMPLVVNGKVLVGGSGGEYGIRGYVVAYDANDGKELWR
                     TFTIPGPGEPGHETWQGDDWKSGGGSAWMTGNYDPALNLVYWGVGNAAPWPGDLHPGD
                     NLFTTSVVALDPSSGTIKAYHQYHPNDSWDWDEVEAPMLIDLQKDGQTIKSLVHPGRD
                     AIFWVLERSPDKI"
BASE COUNT          353 a          549 c          546 g          274 t
ORIGIN      
        1 tcgacgtcta cccgatcgac acggcgcgat tgcgccgcgt cgtcgagctc gtcgccgaca
       61 aagcggagtg gggcaagaaa ttgccgccgc gccacggcca agggatcgcg gtgcaccgca
      121 gcttcgtgag ctacgtcgcg accgtcgtcg aggtggccgt ggacgacaag gggcgtctct
      181 cggtgccacg cgtcgacacc gcgatcgatt gcggttttgc cgccaacccc gaacgcatct
      241 actcgcaggt cgagggcgcg gccgtgatgg ggctgagcct cgccaaatat ggcgagatca
      301 gcttcaagaa cgggcgcgtc gaacagaaca acttcgacgg cttccaggtc cttcgcatcg
      361 acgaatcgcc gccgatcacg catgtctgga tcgtgcccaa cgggatcgac gtgccttcga
      421 gcggcgtcgg cgagccgggg ctgccgccct tcgcgccggc tttgtgcaac gctatcttcg
      481 ccgcgaccgg caagcggatc cgccgcctgc cgatcgccga ccagctggcc atttgacgga
      541 aggcgccaaa atcgcgacgg cggcggttcg ggcggcaggt ttattggaaa cgcgtggtga
      601 cagagcaata gggccgatct ccgtgctgta acgaccgcag ctccgatcac gcagtcgatt
      661 ttcgacaagg gaggaaaccg atgcgaacgc gacaatcgat gtggacgggg ttggcgacgg
      721 cgtcggcctt atggtcggca atagcggctg cgggcgaggt cggcaattac agcccggtca
      781 ccgccgaccg gctcaccaac cccgaacccg ccaactggat gctctaccgc cgcacctatg
      841 acggccaggg ctacagcccg ctcgacaaaa tcaatacagg caacgtcaag aatttggtgc
      901 cggcttggac attttcgacc ggcgtgaacg aaggccacga ggccccgccg gtcgtcaaca
      961 acggcatcat gttcgtggca accccgcaga accaggtgat cgccctcgac gcgaagaccg
     1021 gggaccagat ctggcggtac aaacgggagc tgcccgaaga cctctcccag ttgcatccga
     1081 cgagccgcgg tgtcgggctc tggcaggaca agttgtacct cgccactacc gacgatcacc
     1141 tcgtggcgct cgacgccaag accggcaagg ttgcctggga ccagaaggtc caagattaca
     1201 agaagggtca gtacttgacc ttgatgccgc tggtggtcaa cggcaaggtt ctcgtcggtg
     1261 gctcgggcgg cgaatatggc atacgcggct atgtcgtcgc ctacgacgcc aatgacggca
     1321 aggaactgtg gcgaaccttc accatccccg ggccgggcga gcccggccac gaaacgtggc
     1381 agggcgacga ctggaagagc ggcggcggct cggcctggat gaccggcaat tatgatcctg
     1441 ctctgaacct ggtatactgg ggagtcggca acgccgcacc ctggcccggc gacctgcatc
     1501 caggcgacaa cctattcacc acctcggtcg tcgcgctcga tccgagctcc ggaacgatca
     1561 aggcctacca ccaataccac ccgaacgatt cttgggactg ggacgaagtc gaagccccga
     1621 tgctgatcga tctgcaaaag gacgggcaga cgatcaagag tctggtccac cccggccgcg
     1681 acgcaatttt ctgggtgctg gagcgcagcc cggacaagat ca
//
LOCUS       DATAQL010000997         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3919048, whole genome shotgun sequence.
ACCESSION   DATAQL010000997 DATAQL010000000
VERSION     DATAQL010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3919048"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1966)
                     /locus_tag="VHS97_17235"
     CDS             complement(200..1966)
                     /locus_tag="VHS97_17235"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX3450002.1"
                     /translation="MVVSKQECDRYVPGAGVFSVGRGRCDHVLRANIAAGLGILILIN
                     SGCYKEKDRLPPQDAASVSALVFRSQELPFLYERGETGAAWPVETTGGGMGLLDYDGD
                     GRLDLFFAQGGPLLASQDKATQPVADVLLRNLGGGRFEDVSARVGLAPKGYGQGVTVA
                     DYDGDGDPDVYVTRYGRNTLWRNDRQRGRFTDVTDLAGVGCGSWSLGAAFADYDNDGD
                     LDLFVANYFAFDPARAPFRRDPATGAADYGLPQEFPGLADVLYRNEGSGHFRDVTASA
                     GIAGSGRGMGVLAADFDGDGRIDWLVANDAQSNALWHNRGDGTFEDLAEQLGVAVNGQ
                     GVAEANMGIAYGDTDGNSLPDIMISHFFGEHHTLWRAFAGPSGSIFYQDQTSEAGLAI
                     DSRPLTGWGTVLADLDLDGDLDLVVTNGHIRREPTQVYPYENPPIIWQNKGHGRFANV
                     TTGAGPYFQALHMGRGLACGDLDGDGDLDLVVVHHHAASTILWNESPRKGGHFIVKLQ
                     GRGANRDAIGTRLVAHVGSRRFVRTIDGGGSYLSTNDPRVFFGLGETTQVDRLEVHWP
                     SGKVETRLNLKVDTTVECIEGD"
     gene            complement(2038..2658)
                     /locus_tag="VHS97_17240"
     CDS             complement(2038..2658)
                     /locus_tag="VHS97_17240"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3450003.1"
                     /translation="MATVPTLTTTLPTVQEWKDMLEEILPPQGRWSEDEYLVLTDHRD
                     RLVEFTDGFLEILPMSTDKHQSVLKFLFLAFYGFAEPRGGKVHFAPLRLQIRPGKFRE
                     PDLLLLLSAADPRRENRFWLGADIALEVVSDEMPERDLTDKRGDYAEGRVPEYWIANP
                     QTETITVLRLGADAYEEAGIYRRGESAASTLLAGFSVAVAAVFDAD"
     gene            3182..3340
                     /locus_tag="VHS97_17245"
     CDS             3182..3340
                     /locus_tag="VHS97_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450004.1"
                     /translation="MRPTIRIAASRSAARRLMACGAVITAALWAHLIYTSWRDDGLIQ
                     NLGGDFTL"
BASE COUNT          706 a         1049 c         1036 g          665 t
ORIGIN      
        1 gaggctgccg caccgtttcg aaggatcctc ctccaagtgc tgaccgataa tccgggctca
       61 atcgaggtgg aaaacgccta catgaacgag ttccgccagc gactcagagc agtcgtggaa
      121 cagagccctg caccgcaaac gcggatctca gctctggaaa agtgatcgaa ccggggtacg
      181 aatggatgtc gtcgccgaat taatcgcctt cgatacactc cacggtcgtg tcgactttca
      241 ggtttaatcg agtttcaacc tttcccgagg gccaatggac ctccaggcga tcgacttggg
      301 tggtctcgcc caaaccgaaa aagacccgcg gatcgttagt ggagagatag ctgccgccgc
      361 catcgatggt gcggacaaag cgacgcgagc cgacgtgggc gacaaggcgg gtgccgatgg
      421 cgtcgcgatt ggcgccacga ccttgaagct tgacgatgaa atggccccct ttgcggggtg
      481 attcattcca gaggattgta ctggcggcgt gatggtgaac gacgaccaag tcaaggtcgc
      541 catcgccgtc gaggtcgccg caggcgagcc cgcgtcccat gtgtagtgcc tggaaataag
      601 ggccggcgcc ggtggtgacg ttggcaaagc ggccgtgtcc cttattctgc cagatgatcg
      661 gcggattctc gtagggatag acctgagtgg gctcgcggcg gatatggcca ttggtcacca
      721 ccaggtcgag atcgccgtcg aggtccagat cggccaaaac cgtaccccaa ccggtcagcg
      781 gcctgctgtc gatcgccagg cccgcttcgc tggtttgatc ttggtaaaag atggatccag
      841 acggtccggc gaaggctctc cagagtgtat ggtgctcgcc aaagaagtga gagatcatga
      901 tatcgggcag gctgttgccg tcagtgtcgc cgtaagcgat tcccatgttg gcttcggcga
      961 ccccttggcc gttgactgcc acgccgagct gctcggccag gtcttcaaac gtgccgtcgc
     1021 cccgattgtg ccagagggcg ttactctgcg cgtcgttggc gacgagccag tcgatccggc
     1081 cgtcgccatc gaaatcagca gccaatacgc ccatgcctcg tcccgacccg gctatgccgg
     1141 cggaggcagt gacatcgcgg aagtgcccgc ttccctcatt gcggtaaagg acgtcggcaa
     1201 ggccggggaa ctcttgcggc aacccgtagt ccgctgctcc cgtcgccgga tcgcgccgaa
     1261 agggtgcacg ggcaggatca aacgcaaagt agtttgccac gaaaagatcg aggtcaccgt
     1321 cgttgtcgta gtcggcgaat gccgccccca ggctccagct tccgcagccg acgccggcta
     1381 ggtcggttac gtcggtgaaa cgaccgcgct ggcgatcgtt tcgccagaga gtgttgcgac
     1441 cgtagcgggt tacataaaca tcaggatcgc cgtcgccgtc gtagtcggcc acggttactc
     1501 cctgtccgta acctttgggg gcgaggccga cacgtgctga gacgtcttca aagcggcctc
     1561 cgcccagatt cctcaataac acatcggcaa caggctgagt tgctttgtcc tggctcgcca
     1621 gcagcggtcc cccttgcgcg aaaaagagat cgagccggcc gtcgccgtcg taatcgagga
     1681 ggcccatccc accccccgtc gtttcgaccg gccacgccgc gcctgtttca ccccgctcgt
     1741 agagaaacgg cagctcttgc gagcgaaaaa ccagcgcgct aacggacgca gcatcctggg
     1801 gcggcaaacg atctttttcc ttgtagcagc cggaattgat cagaatcaat atgcccaatc
     1861 ccgcggctat gttcgcgcgc aagacgtgat cgcatcgtcc tcgacccacg gaaaacaccc
     1921 ccgcaccagg gacgtatcga tcgcattcct gcttagaaac caccatcact ctgcgcctgc
     1981 tccttggcaa gctcatcgaa tcggctgtaa cgctcatggt ggttcacggc cgttgattca
     2041 atccgcgtcg aaaaccgcgg caacggcgac cgagaagcct gccaaaagcg tcgaagcagc
     2101 cgactcccca cgccggtaga tgcctgcttc ttcataagca tccgcgccca aacgaagaac
     2161 cgtgatggtt tcggtttgcg gattcgcgat ccagtattcc gggacgcgac cctcggcata
     2221 gtcgccccgc ttgtcagtca gatctcgttc tggcatctca tcgctcacaa cttccagcgc
     2281 gatgtcggcg cccagccaga aacgattctc tcggcgcgga tcggcggctg aaagcagcaa
     2341 tagcaaatcg ggctcgcgga acttgcccgg ccgaatttgc agccgcaacg gggcgaaatg
     2401 gactttgcca ccgcggggct ctgcgaaacc gtaaaaggca agaaacaaga acttcaacac
     2461 gctttggtgc ttgtccgtgg acatggggag gatttccagg aagccgtcgg tgaactcgac
     2521 cagccggtcg cggtggtcgg tcagaacgag atactcgtcc tcgctccatc gcccctgggg
     2581 cgggagtatt tcctcgagca tgtccttcca ttcttgtacc gtcggaaggg tcgtcgtaag
     2641 agtcggtaca gtcgccatgg cgtccccctt gagaaagggc ttaacttccc tagctcagta
     2701 tagtcgcaaa tggcacgctc cgcgatgtgt atgagggtcc ggcgagcgag tcgggcggct
     2761 ctgcgacaag gtaatgatca tccgactcgg cgaagatcgg accacacgcc tctcacctgg
     2821 tccgaaatga gcgctcagcg acccaagcgg tgctcgcctc gatctgcgga ttcgcactga
     2881 agaaggtctg aacccaaatt acgagtatga cggcggccct ggctctctgt cccgtagtca
     2941 ccgccccgag cgacagcgaa tcagggctta attcaagcgg aaatcgccgc gcgtcctgct
     3001 tgccgacatc aatgggggcg gcgtatgatt cggcgtcgca ggtataggac cgaaaatcta
     3061 acgctgataa gacccgcact cgctggccca tcgaacgagg cgggcaaacg agtgcggcag
     3121 tagccttggt gaaccgcagc cctgggcaga accgctaaat cagaaggttt tggagcctga
     3181 attgcggcca accatccgga tcgccgcatc gcggagtgca gcccggcgac taatggcatg
     3241 cggcgcggtg atcacggccg cgctttgggc ccaccttatc tacacatcct ggcgcgatga
     3301 cggtctcatc cagaacctgg gcggcgactt tacattgtga tccataccgc gctggcgttt
     3361 gtcgcaatgc tggtgctctt gcgatcgtgg gaaataagct ggtcgggatc ggggctgggc
     3421 gcgctgagct acacgttcgg tgtgccgatc ctgttt
//
LOCUS       DATAQL010000998         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3766080, whole genome shotgun sequence.
ACCESSION   DATAQL010000998 DATAQL010000000
VERSION     DATAQL010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3766080"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /gene="rpsM"
                     /locus_tag="VHS97_17250"
     CDS             <1..229
                     /gene="rpsM"
                     /locus_tag="VHS97_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX3450005.1"
                     /translation="LAKLASLLDNEYTVEGQLRRQVQQNIARLRDIGCYRGLRHRKGL
                     PVRGQRTRTNARTRKGPRKTVAGKKGVKELR"
     gene            477..857
                     /gene="rpsK"
                     /locus_tag="VHS97_17255"
     CDS             477..857
                     /gene="rpsK"
                     /locus_tag="VHS97_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX3450006.1"
                     /translation="MAKAKKRKTRRNVSRAVVHIKATFNNTTVTITDPNGDALCWASS
                     GTVGFKGSRKSTPFAAQRAAETAAASATKYGVKEVEVKVKGPGSGRESAITAIQASGL
                     LIKAIEDVTPLPHNGCRPPKKRRV"
     gene            1028..1657
                     /gene="rpsD"
                     /locus_tag="VHS97_17260"
     CDS             1028..1657
                     /gene="rpsD"
                     /locus_tag="VHS97_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX3450007.1"
                     /translation="MGRHVGPVCRLCRREGTKLFLKGTRCDTPKCAVDRREGVPGMHQ
                     FRRSKASEYARRLREKQKVKRYYGIFERQFRKYYEEASRRPGNTGDALMSLLERRLDN
                     VVTRLGFAASRPQARQMITHGHLSVNGRKLDIPSYLVRPGDKIELKNRDHSRKLATEN
                     LAMGSGPPVPEWLDRFSTDPPEGRVSRLPVVQDVGLPVTPQLIIELLSR"
     gene            1701..2702
                     /locus_tag="VHS97_17265"
     CDS             1701..2702
                     /locus_tag="VHS97_17265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX3450008.1"
                     /translation="MRIRWRGLELPSRVICNRETLTDTFGEFHVEPFERGFGHTVGNS
                     LRRILLSSLEGSAITKIKILGVQHEFSSIPGMVDDITDLVLNVKLLVVKNHSESSRTI
                     RIDRDRRGVVTAADILHDESVEIINPDHILCTLTDDVPFHLEMTVENGRGYRTAAEGH
                     TDDLEIGVIPIDASFSPVQRVEYHVENTRVGQRTNYDKLILRIWTTGVLGPEMGLVEA
                     AKILRKHLNPFVQYNEPGPGLPSEALSGDGSQHLPIDAELERKLDMSLAELELSVRAT
                     NCLESEGITTVRHLVSRSEDQLLNVRNFGETTLKEVRAKLHEIGLDLGMNVPMKPGA"
     gene            2756..3175
                     /gene="rplQ"
                     /locus_tag="VHS97_17270"
     CDS             2756..3175
                     /gene="rplQ"
                     /locus_tag="VHS97_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX3450009.1"
                     /translation="MRHQKAGRKFKRSPSHRRMLLRNLATALLEHGKIQTTLAKAKEL
                     QPFTEKLITLAKDGWNLDHFRRSLAVLTSKEVAFRLFHEIGPRYKDRSGGYTRIYKLA
                     KVRQGDAAPMAVISLLGESETIKAPAKPAVVGSSRGA"
     gene            3418..>3570
                     /locus_tag="VHS97_17275"
     CDS             3418..>3570
                     /locus_tag="VHS97_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX3450010.1"
                     /translation="MSHRPHDPHCLFCNIAHGTIPSAKILETAEAVAFLDINPVNRGH
                     TLLVPRA"
BASE COUNT          786 a         1109 c         1032 g          643 t
ORIGIN      
        1 cctggccaag ctggcttcac tcctggataa cgagtatacg gtggaggggc agctccgccg
       61 ccaagtgcag caaaacatcg cccgtttgcg cgacatcggc tgctatcgcg gtctgcggca
      121 ccgtaaggga ttgccggtgc gcggccagcg gacccggacc aatgctcgga cccggaaggg
      181 cccccgcaaa acggtcgctg gaaagaaggg cgtaaaggaa ttgcggtaac gaaaccggca
      241 tgctttccgg tctcttttca agcggatatt taccattcag gcggcctggc cgtctggtcc
      301 gacccgacca tgccgccgtg gattggatat tgtccgccgg cctcgggtga cctggcccgc
      361 cctctgacgg gttggacaga gccggtcgaa gagtgccggc attttgctat tttcctcgcg
      421 agtgtcaccc aagattaaaa tagttagata cagatatctc aaggagcgac ggacacgtgg
      481 ccaaggccaa gaagcggaag actagacgca acgtcagtcg ggcggtcgtg cacatcaagg
      541 cgacgttcaa caacacgacg gtgacgatca ccgacccgaa tggcgatgcg ctctgctggg
      601 cctcgtcggg aaccgtcggc ttcaaaggga gccgcaagag cactccattc gccgcgcagc
      661 gggcggcaga gaccgccgcg gcgtccgcaa ccaagtatgg cgtcaaggag gtcgaggtca
      721 aggttaaggg gccgggctcc gggcgcgaaa gcgcgatcac ggcgatccag gcctcggggc
      781 tcttgatcaa ggccatcgaa gacgtcacgc cgctgccgca caacggctgt cgtcctccca
      841 agaagcgccg cgtatgacaa ccgcgtgttc gatcgcgacc tggcacgaag tcactctacc
      901 gcaaccggag aattgatcga atccatccag aaccgatagt gcggccgaga ggccggtcgg
      961 ctggatctca ccgataacga gaagttgatt ccacgacttc aactcgaccg agggccgttc
     1021 ttccgccatg ggacgtcacg ttggaccagt ttgccggctc tgccggcgcg aaggcaccaa
     1081 gctctttctc aaaggaacca ggtgcgacac gccgaagtgc gccgtcgatc gtcgcgaagg
     1141 cgtaccggga atgcatcagt ttcgccgcag caaagccagt gaatacgcca gacgcctgcg
     1201 agagaaacag aaggtcaagc gctattacgg aattttcgag cgccaattcc gcaagtatta
     1261 cgaagaggcc agccggcgcc cgggtaacac cggtgatgcg ctgatgtcgc tcttggagag
     1321 acggctggac aacgtggtga cgcggctcgg attcgccgcc agccgccccc aggcgcggca
     1381 gatgatcacg cacggacacc tctccgtgaa cggtcgcaag ctcgacatac ccagttacct
     1441 tgtccgtccc ggtgacaaga tcgaattgaa gaatcgcgat cactcccgca agctggctac
     1501 ggaaaacctt gcaatgggta gtggaccgcc ggtccccgaa tggctggatc gtttcagcac
     1561 cgacccgccc gaaggtcgcg tgagccgctt gcccgtcgtc caggacgtcg ggctgccagt
     1621 gacaccgcag ttgatcatcg agttgttgag ccgctgacct cgcgcggtga agcgcttcaa
     1681 attgggatgg ggagatagtg atgcgtattc gttggcgagg cctcgaacta ccgagccggg
     1741 tcatctgcaa ccgcgagacg ttgaccgata cgttcggcga gtttcacgtc gaacccttcg
     1801 aacgcggctt cggccatacc gtcggcaaca gtcttcggcg cattctcctc tccagtctgg
     1861 aaggaagcgc catcaccaag ataaagatcc tcggtgttca gcacgagttt tcctcgattc
     1921 ccgggatggt cgacgacatc accgatctcg tgctcaatgt caagctgttg gtcgtcaaga
     1981 atcactccga gtcgtcgcgc acaatccgga tcgatcgcga tcgtcgcggc gtcgttacgg
     2041 ccgccgatat cttacacgac gagtcggtcg agattatcaa ccccgaccac attctctgca
     2101 ccctgaccga cgacgttcct ttccacctgg agatgaccgt cgaaaacggc cggggttacc
     2161 gtaccgcggc cgagggacac accgacgatc tcgagatcgg cgtcatcccg attgacgcca
     2221 gcttcagccc ggtgcagcgc gtcgagtatc acgtcgagaa tacccgcgtc ggccagagga
     2281 ccaactatga caagctgatc ctgcggatct ggacgaccgg agtcctgggc ccggaaatgg
     2341 gtttggtcga ggccgccaag atcctccgca aacatttgaa cccgtttgtg cagtacaacg
     2401 agccagggcc cggcctcccg tccgaggcac tctctggcga cggctctcag catttgccca
     2461 tcgatgccga gctcgaacgc aaactcgaca tgagcttggc cgagctcgag ctgtcggtcc
     2521 gtgcgaccaa ctgcctcgaa agtgagggaa tcacgaccgt ccggcacctg gtcagccgta
     2581 gcgaagatca actgttgaac gtccgcaact tcggtgaaac caccctcaag gaggttcgcg
     2641 ccaagttgca cgaaatcgga ctggatctgg gaatgaacgt gcccatgaag ccaggtgcat
     2701 aggttccatt ccactggcca agcgccatta accaatagcc caatcattca aaaccatgcg
     2761 tcaccaaaaa gccggccgaa aattcaaaag gtccccctcg catcgccgga tgctgctgcg
     2821 caacctcgcg accgcgctct tggaacacgg caagatccag acaactctgg ccaaagccaa
     2881 ggaactccag ccgttcaccg agaaactgat caccctggcc aaggacggat ggaaccttga
     2941 ccatttccga agatcgctgg cggttctcac cagcaaggaa gtggcgttca ggctcttcca
     3001 cgagattggg ccccgctaca aagaccggtc agggggctac acgcggattt acaaactggc
     3061 caaagttcgc cagggagatg ccgctcccat ggccgtgatt tcgcttttgg gcgagtccga
     3121 gacgatcaaa gcaccggcca agccggccgt cgtcggttca tctcgcggcg cctgatcaaa
     3181 ccgcttttcg acgggctggt gcacgagcct gtacagtcat ggaccgaacc ataagtcagc
     3241 tttacgcctc gcgcgacgcc agatgagtca tgattttagg gtgggcattg cccaccgacg
     3301 cgggcttggg cgcacagaat cggtgggcaa tgcccaccct acgataactc gctcaacacc
     3361 gcgtcgagca tagttcaccc gaatcagggt ttgagtgcga cacaggggca caatatcgtg
     3421 agccatcgac cgcacgatcc acattgccta ttctgcaaca tcgctcacgg caccatccct
     3481 tcggccaaaa ttctcgagac cgccgaggcc gtggccttcc tcgatatcaa tccggtcaat
     3541 cgcggccaca ccttgctcgt gccccgggct
//
LOCUS       DATAQL010000999         3747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2086235, whole genome shotgun sequence.
ACCESSION   DATAQL010000999 DATAQL010000000
VERSION     DATAQL010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086235"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..2674
                     /locus_tag="VHS97_17280"
     CDS             314..2674
                     /locus_tag="VHS97_17280"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450011.1"
                     /translation="MSRISTAAATAPALAKAPSRTRHSHVVLGQTPTPYTDLFFISFA
                     TLFLELACIRWFGSAVLYLTFFTNLVLFACFLGMSVGLLAASRRVDFATWIIPLAVAA
                     VGLAELTSWAYVHFGNVSIEVGGQSSPQQVFFGTETPRGDLSRFVVPVECVAAVFFTV
                     IALMFIGLGQTMGRAFAAASDRVSAYSVDIIGSLAGIACFSLASYWQTSPVVWFAIGI
                     GATFRALPRVSRIQFYGLLALLALLAAAATRDGGKFLTFWSPYYKVTYHPRSGMIETN
                     HIGHQVIMDVRKDGSAYSLPHLLNRDAGGRPFDDVLIIGAGSGNDVAAALWHGAGAVD
                     AVEIDPSIQRLGRIDHPNHPYADRRVTAHLDDGRSFLKTTERKYDLATYALVDSLVLH
                     SGYSSLRLESFLFTEQAFRDVKARLKPGGAFVMYNAYRQGWVIGRLAALAENVFGTKP
                     IILSMPHVDAINPGDNQGGRVTCLVVGLPDSPIVELVRKAFGTRGSFWLASVPAANIP
                     VNGFGDRPAPAGDRDGLWSEVRPVRVATEGVGPLPTDSWPFLYLREPVIPALNLRGMA
                     LVAGISLVILATFAPVRTARPNGRMFFLGAGFMLLESKGVVHMALLFGSTWVVNAVVF
                     AAILVMILLSNLFVSVVRPRALWPYYLLLGAALVVNALVPMSGFLRLTGLLRLTASCS
                     VVFVPVFFAGVIFAVGLRDSPRPDVALGSNVAGVILGGLAENLALVFGFDHLLLVALV
                     FYFLSALFTTRGSDDGTSGAIASASYVMRSWWQLIIGSSGGRARWG"
     gene            3189..3740
                     /locus_tag="VHS97_17285"
     CDS             3189..3740
                     /locus_tag="VHS97_17285"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3450012.1"
                     /translation="MSVYRRLFIPGGTYFFAVVTYDRRPILTDPLGRSCLRAAIRSVR
                     AKRPFDVIAFVLMPDHLHAVWTLPLGDSDYAVRWSQIKESFTRLFLASGGMEGSRNAA
                     RVRRRERSVWQRRYWEHTCRDEDDLKRCIDYIHWNPVKHGLVERVQDYPWSTFHRFAR
                     LGDYDIDEGIAHSPGDTKNFGWE"
BASE COUNT          633 a         1165 c         1100 g          849 t
ORIGIN      
        1 aagagctcca ctcccggcgc cgcgctctcg ccaccttgat acccttcttt gatcggattc
       61 tacgaccctt ttcctgacaa ccccaggcca agctaaacca attcaaacgg cacgtcttga
      121 cattatcggc aaagacaacc aaagccgcct aagcgtgatg attttcaagc ctcgcaggaa
      181 tgcatccatc ctatcgccat ccgtcaagcg ggtgggatag accacctttg tatttgtcgc
      241 taggatctcc ccactgaatg atctctcaac cgggcacgga tttgcgaggt ccggcaagca
      301 agggattgcc acgatgtcca ggatttcgac cgctgcggct acggcaccgg ctctcgccaa
      361 ggcaccttcg cggacccggc attcgcacgt cgtgctggga cagaccccaa cgccgtacac
      421 cgacctgttt ttcattagct tcgccaccct tttcctggaa ctggcttgca tccgctggtt
      481 tggcagcgcg gtgttgtacc tgactttctt cacgaatctc gtgctcttcg cttgttttct
      541 cggcatgtcc gtcggcctgc tcgccgccag tcgacgggtc gacttcgcta cctggataat
      601 ccctctggcg gtcgccgccg tcggcctcgc ggaactaact tcctgggcct acgtccattt
      661 tgggaacgtt agcatcgaag tcggcgggca gtcgtcgccg caacaggttt tcttcgggac
      721 cgagacgcca cgaggtgacc tcagcaggtt cgtcgtccct gtcgaatgcg tcgccgcggt
      781 gttcttcacg gtcatcgcct tgatgttcat tgggctcggc cagacgatgg gtcgcgcatt
      841 cgccgcggct tccgaccgcg tatccgccta ctcggtcgac atcatcggta gcctcgccgg
      901 gatcgcgtgt ttcagtctgg cttcgtactg gcagacctct cccgtcgtgt ggttcgccat
      961 tggcataggg gcgaccttcc gcgcactgcc gagagtctcc cgaatccagt tctacggcct
     1021 gttggccctg ttggccttgt tggccgccgc cgcgactcgc gacggtggca agtttctgac
     1081 attctggtcg ccgtactata aggtgacgta ccatccgcga agtggaatga tcgaaacgaa
     1141 tcacatcggc caccaagtga tcatggatgt gcgaaaagac ggctcggctt actcgctgcc
     1201 ccacctattg aatcgggacg caggcgggcg acccttcgat gacgtcctga ttatcggcgc
     1261 tggctctggt aacgacgtcg ccgccgcgct ctggcatggg gctggggcgg tcgatgccgt
     1321 cgagatcgac ccgagcatcc agcgactcgg gcgaatcgat catcctaacc acccctacgc
     1381 cgaccgccgg gtcactgctc acctcgacga tggccgcagt ttccttaaga cgaccgaacg
     1441 taagtatgac ctggcgacgt acgcgctggt cgactctctg gtactccact ccgggtattc
     1501 cagtctccgc ttggagagtt tcctattcac cgagcaggcg ttccgcgacg tcaaggcgag
     1561 gctcaaaccg ggcggggcat tcgtgatgta caacgcttac cgccagggct gggtcatcgg
     1621 tcgcctggcg gcgctggcgg agaatgtatt cgggaccaag ccgatcatcc tctcgatgcc
     1681 ccacgtcgac gcgatcaatc cgggagacaa ccaaggtggg cgcgtcacgt gcctagtcgt
     1741 cggcctgccc gattcgccga tcgtcgagct ggttcgcaag gcgttcggta ccaggggctc
     1801 gttctggcta gcgtctgtgc ctgcggccaa catcccggtc aacgggttcg gtgaccggcc
     1861 cgcgccggca ggcgatcgtg acgggctctg gtccgaggtt cgcccggtcc gagtggcaac
     1921 ggagggcgtg ggaccgctgc cgaccgactc ctggccgttc ctctacctca gggagccggt
     1981 catcccagcg ctgaacctcc gcgggatggc tctcgtcgcc ggcatttcgc ttgtcatcct
     2041 ggcgacgttc gccccggtgc ggacggctcg accgaacgga cgaatgttct tcctgggcgc
     2101 cgggttcatg ctccttgagt ccaaaggcgt cgtacacatg gcgttgctct ttggttcgac
     2161 ctgggtggtc aatgcggtgg tattcgccgc gatcctcgtc atgatcctct tgtcgaacct
     2221 gttcgtgtcg gtggtccgtc caagagcatt gtggccctac tatttgctcc taggagcggc
     2281 gttggtggtc aacgccttgg tgccgatgtc tgggttcctc cgtctgacgg gtctactgag
     2341 gctaaccgcc tcgtgctcgg tggtcttcgt acccgtattc ttcgcggggg tgatcttcgc
     2401 ggttgggctt cgggacagcc cgcgtccaga cgtcgccctc ggctcaaacg ttgccggggt
     2461 tatcctcggc gggctggccg agaacctggc gcttgtattt ggcttcgatc acctcttgtt
     2521 ggtcgccctc gtcttctatt tcctgtcagc attattcacc acacgcgggt cggacgacgg
     2581 gacatcgggg gccatagcga gtgcgtccta tgtgatgcgt agctggtggc agctcattat
     2641 tggctcatcc ggcggacgcg cgcgctgggg ttgataattg gtcccaggtc gacgcagagc
     2701 tggcgaaatg ccggggaagt agtcccaagt cggccagtta gtacgaatcg ggacgcgact
     2761 cattcccgcc tgtcggcgac ccatgcgtct cggttatttc ccactgaacc gtccgtgcga
     2821 catctcaact gaagccactg actttacgag tgcacatttg agacgcaccg tgtctcaacg
     2881 gtgggcctct cgccgcgatg cgcggcggtc cgcaccaccc taccgcagga gcgacgcgtg
     2941 cggcatgtag ggtgggccgc ccaggcccac cgggacgcac cgtgtctcaa cggtgggcct
     3001 ctcgccgcga tgcgcggcgg tccgcaccac gctatgttcc caaaaagcct cacttcctca
     3061 attgcgattg gtcgtaatcg agcaacgcca ccgtggaagc gcgtttcgcc ggttatgttg
     3121 tattgtcgtc gaatagaggc cgtgtagagc agcggcttag gttcaagaaa tacccagagt
     3181 tttcattgat gtctgtctat cggcggttat tcatcccggg agggacgtac ttcttcgcgg
     3241 tcgtgactta tgatcgccgg cccattctca cagatcctct cggccgatcc tgtcttcgtg
     3301 cagcgatacg aagtgttcgg gccaaacgcc ctttcgatgt aatcgcgttt gtcctcatgc
     3361 ccgatcatct ccatgccgtc tggaccttgc cgcttggcga ttcggactat gctgtgcggt
     3421 ggtcacaaat caaagagtcc ttcacccgac tttttctggc atccggtggg atggagggct
     3481 ctcgaaatgc cgcgcgcgtg cgtcgccgcg agcgatcggt ctggcaaaga aggtattggg
     3541 agcatacgtg ccgcgacgag gacgatctca agcgatgcat tgattacatc cactggaatc
     3601 ccgtcaagca cgggctggtc gaacgcgttc aggattaccc gtggtcaaca ttccaccgat
     3661 tcgcccgtct cggagattat gacatcgacg aaggaatcgc ccattctccc ggcgacacga
     3721 agaactttgg atgggaataa tgcggcc
//
LOCUS       DATAQL010001000         4244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4223614, whole genome shotgun sequence.
ACCESSION   DATAQL010001000 DATAQL010000000
VERSION     DATAQL010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4223614"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..876)
                     /locus_tag="VHS97_17290"
     CDS             complement(262..876)
                     /locus_tag="VHS97_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3450013.1"
                     /translation="MDGNVRTADPVKIERIIDAAAQLFAERHYHEVRMDDIAAKAGVA
                     KGTIYLHFKDKDDLYQALALDSLKKLSKRIRDGLVGVDDPATKLLSVNREVIQYFEHR
                     AFTLDLIIRERLQEQEHGANKAFFEVRDEFMRILHSILDEFPEAGHRSEADISLAVLA
                     LSGISREVLQRLPRPWPANLPERLTRLFLEGFLRPGTSETARSS"
     gene            1172..2800
                     /locus_tag="VHS97_17295"
     CDS             1172..2800
                     /locus_tag="VHS97_17295"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3450014.1"
                     /translation="MSREGKTAEESSGQITESTSVNAASPGHLNVTGPAVEAARDQTR
                     GRWSRVIFLGVGMVASALFFWKGLPFIREYLSHAETNDAYVTGEPSAVGSRITEVVKK
                     VFVKNNDFVEQGTLLVTLDRAMLEVQADQKRAELRQEELRLEQLAKSLETNRASLDLA
                     RDKVGIGIASLIESSKTIEEKQLQVRYRVASLRAAAASLRSAQVDLALAQKEFNRVDH
                     LVAKQSATQAELDQKRSVLDSARERVKAAEQQVQQVRAQLAISPNYQQPDQIPDDLER
                     TDTEVRKSVATGQQILAQLGISFGRNLDPMVFYKVVSVTLARPQGWIDQISSVREAQG
                     QLDQTLAALGGKSFDPSRLYQHPKIMKLRKELEEAELKLSYTEIRAPVTGVVNRKSVN
                     PGDHVQASQALMAIQPLETVYIVANFKETQLSDILIGQPVEIYVDAYPGRPLKGRVTG
                     FAAATGAASSLLPAENATGNFVKVVQRLPVRIDLAEPNPRKTPLFVGMSVIPEVDIKA
                     MPTGPDAGERLRSSEAVRAAASVNEASPTREVMR"
     gene            2797..>4244
                     /locus_tag="VHS97_17300"
     CDS             2797..>4244
                     /locus_tag="VHS97_17300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3450015.1"
                     /translation="MSANTDVPADAAAYTPRFNPWLIAITVTMATFMEVLDTSIANVA
                     LPHMAGSLAVSTEESTWVLTSYLVSNAIILPLSGWLSSVLGRKRYYMISVFLFTIASA
                     LCGFATSIEQLVLFRVLQGLGGGGLQPSEQAILMDTFAPSKRGMAMAVYGVSILCAPV
                     LGPTLGGFITDNYSWRWIFYINVPVGILSLILSGIVVEDPPYLKKMKAERASHKHRVD
                     FIGLGLLTIGLASLELALDKGQTYDWFGSRFIVRALILAVVALVAVVIYELWHPDPII
                     NLRLFKDRNFAMASVGVFCAFAVLYGSTVLLPQLLQTLMGYTPMNAGLVLSPAGLVTM
                     LVMPMIGIALSKGVDARWLIVLGLTIVGSTCLWYASLNLNVAPGQVMWPRIWQTVGAG
                     LLWVPINTAAYAYIAKEQTSNASGLFNLIRNEGSSIGVAMTNTLLQRRTQFHHSRLVE
                     GLDRYNPVDMQWLDGAAGTMRAVGSDPALAAR"
BASE COUNT          852 a         1208 c         1322 g          862 t
ORIGIN      
        1 gaacctgctc gatgccgatc ccgaccagga aatcggtcat ccgcttaaag ttcttatcca
       61 tcgttcaccc tcgtcgaaga tgttggcaaa tgacggtgcg gacgggccct cgtcaagcta
      121 gccggatgac tgcgagggcg atcataacgg gctcgcgacc agattgcgag tttgccgcga
      181 agcgcggcaa gcaaagtggg accgttaaag ctcgcgtccc cttatccagt ttgccgaagt
      241 tcgtcccgga tttgtatcgc tctatgaaga ccgggcggtc tcggacgttc cggggcgaag
      301 gaagccttcg agaaagagcc gggtgagccg ttcgggaagg ttggcgggcc aaggacgagg
      361 cagtcgttgc agcacctccc tacttattcc cgaaagggcg aggaccgcca gggaaatgtc
      421 cgcctcgctc ctgtgaccgg cttcgggaaa ctcgtcgaga atcgaatgaa gaatcctcat
      481 gaattcgtct cgaacttcaa agaacgcctt gttggcgccg tgctcttgtt cttgaagccg
      541 ttcgcgaatg atcagatcca acgtaaaggc gcggtgttcg aagtactgaa tcacctcccg
      601 gttgacgcta agaagcttgg tcgcggggtc gtcaacgccc accaagccgt ctcggattct
      661 tttgctgagc ttcttgaggc tatcgagcgc gagggcctga tagaggtcgt ctttatcctt
      721 gaaatgcaga tagatcgtgc ccttggcgac acccgccttc gccgcgatat cgtccatgcg
      781 gacttcgtgg taatgccgtt cggcgaataa ttgcgccgcg gcgtcgataa tgcgttcaat
      841 tttgaccgga tcggccgttc gcacatttcc gtccatcagt cacatcgagc agcatatcta
      901 gcgacgatag caatcgtcct ttcgtccaac aaacgaaaga gatcgatttt ccctcatttt
      961 aacaaagaga ttccgcgaac gctgaagcga aactcccggt tctctcggtt ttccaactcg
     1021 atacgccacc cgcatttcag tctcgaacgg tcaattcgat caaatctctc tggtcgtgga
     1081 tgacaaacct aatacaataa ctgacctgac cggtcagtct agttatgagc ttgtcggctc
     1141 aggggcgttc taaccgcggg agaagcacgt tatgtcgcga gaaggcaaaa cggcggaaga
     1201 gtcgtccgga cagatcacag aatcaacatc ggtcaacgcg gcgtcgccag gacatttgaa
     1261 cgtgacgggt ccggcggtcg aggcggcgcg ggaccaaacc cgtgggcgtt ggtcgcgggt
     1321 gatcttcctc ggtgttggaa tggttgcgtc ggcccttttc ttttggaagg gcctgccgtt
     1381 cattcgcgag tatctttcgc atgcggaaac caacgacgcc tatgtgacgg gagaaccctc
     1441 ggcggtggga tcgcggatca cggaagtggt gaagaaggtg ttcgtcaaga ataacgattt
     1501 cgtggagcag ggcacgcttc tggtcacgct cgaccgcgcc atgctggaag ttcaagccga
     1561 tcagaagcgc gcggagctga ggcaggaaga gcttcggttg gagcaattgg cgaagtcgtt
     1621 ggagacgaac cgggcgtcgc tcgacctcgc gagagacaag gtcgggatag ggatcgcctc
     1681 acttatcgag tcaagcaaga cgatcgagga gaaacaatta caggttcgct accgagtcgc
     1741 gagccttcgg gcggcggcgg cctcgctacg gtccgcccag gtggatctgg ccctggcgca
     1801 aaaggaattc aaccgggtgg atcacttggt cgccaagcag tcggccactc aagcggagct
     1861 cgaccaaaaa agatcggtac tcgacagcgc tcgcgaaaga gtcaaagccg ccgagcaaca
     1921 ggtgcagcaa gtacgggcgc aactggcgat ttcgcccaac tatcagcagc cggaccagat
     1981 tccggacgat ctggagcgaa cggataccga ggtccggaaa tcggtggcga ccggtcagca
     2041 aatcttggct cagcttggaa tttccttcgg caggaacctg gacccgatgg tgttctacaa
     2101 ggtggtttcc gtgacactcg cccgtccgca gggctggatc gatcagattt cctcggttcg
     2161 ggaagctcag ggccagttgg atcaaaccct ggccgctctc ggcgggaagt cgttcgaccc
     2221 gtcacggctg tatcagcatc ccaagatcat gaaactgcga aaagagctcg aggaagccga
     2281 gctcaagctg agctataccg agattcgcgc cccggtgact ggcgtcgtca accggaagtc
     2341 ggtcaatccg ggcgaccacg tgcaggcgtc ccaggcgctc atggcgattc agccattgga
     2401 aaccgtttac atcgtcgcga acttcaagga gacccagctc agcgacatct tgatcgggca
     2461 accggtcgag atttacgtcg acgcctaccc gggtcgtccg ctcaaggggc gcgtgaccgg
     2521 attcgcggcg gccaccggag ccgcgtcgtc gctcttgcct gcggaaaacg ccacgggcaa
     2581 cttcgtgaag gtcgtccagc ggttgccggt gcggatcgac ctggcggagc cgaacccgcg
     2641 caagacgccg ttgttcgtgg ggatgtcggt cattccggaa gtcgacatca aggcaatgcc
     2701 aacggggccg gacgcggggg aaaggctgcg cagctcggag gctgttcgag cggcggcgag
     2761 cgtcaacgaa gcttcgccca ccagggaggt catgcgatga gtgcgaacac ggacgttccc
     2821 gccgacgcgg ccgcgtacac gcctcgattc aatccctggc tgatcgcgat caccgtgacg
     2881 atggcgacct tcatggaggt actggacacc tcgatcgcca acgtcgcctt gccgcacatg
     2941 gcggggagcc tcgcggtgtc gaccgaggag agcacgtggg tcctcacgag ctacctggtg
     3001 tcgaacgcga tcatcttgcc gctctccgga tggctttcgt cggtcctggg acggaagcgg
     3061 tactacatga tctccgtctt tctcttcacc atcgcctccg ccctttgcgg gttcgccacc
     3121 agcatcgagc aactggtgct gttccgggta ctgcaaggct tgggcggcgg cggtctgcaa
     3181 ccgtccgagc aagccatcct gatggacacg ttcgcgcctt ccaagcgagg catggcgatg
     3241 gcggtctacg gggtgtccat cctgtgcgcc ccggtcctgg gcccgacgct cggcggattc
     3301 atcacggaca actattcctg gcggtggatc ttttacatca acgtgccggt gggcattctc
     3361 tcgctgattc tcagcggaat cgtggtggaa gacccgccgt acttgaagaa gatgaaggcg
     3421 gaacgggcaa gccataagca tcgagtggac ttcatcggcc tggggctgtt gacgatcggt
     3481 ctggcatcgt tggagctggc cctggataag ggccaaacct acgactggtt tggctctcga
     3541 ttcatcgtcc gagcattgat cttggcggtg gtcgccctgg tcgcggtggt gatttacgag
     3601 ctatggcatc ccgacccgat catcaacctc cgcctcttca aggaccgcaa cttcgccatg
     3661 gcctcggtcg gcgtcttttg cgcgttcgca gtgttgtacg gcagcacggt gttgctcccg
     3721 caattgctgc aaactctgat ggggtacacc cccatgaacg cggggctggt gctctcgccg
     3781 gccgggctgg tcacgatgct ggtgatgcca atgatcggga tcgcgctatc gaagggggtg
     3841 gacgcgcggt ggctgattgt gctggggttg acgattgtgg ggtcgacttg cctgtggtac
     3901 gcgtcgctca atctcaacgt cgctcccggg caggtgatgt ggccgcgcat ctggcaaacg
     3961 gtaggggccg gccttctctg ggtacccatc aatacggctg cgtatgcata tattgctaag
     4021 gagcagacca gtaacgcgtc gggcttgttc aacttgatcc ggaacgaggg gtcgagcatc
     4081 ggcgtggcga tgacgaacac gttgttgcaa cgacggaccc aatttcatca cagccgattg
     4141 gtcgaagggc tcgaccgcta taacccggtg gacatgcagt ggctcgatgg ggcggccggc
     4201 acgatgcggg ccgtcggctc cgatcccgcg cttgcggccc gcca
//
LOCUS       DATAQL010001001         7426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2086415, whole genome shotgun sequence.
ACCESSION   DATAQL010001001 DATAQL010000000
VERSION     DATAQL010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086415"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VHS97_17305"
     CDS             <1..620
                     /locus_tag="VHS97_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450016.1"
                     /translation="QGEESGPGQPGERGQQAQQGQEGEQGQQGQQGQQGQQGQQGQQG
                     QQGQGQSQAGNPGQRGQGQQQGRQANSLRGGGGADRQGGPDRQLGGGPGGPITGEGFR
                     QWSDRMRDVEELLDDPEMRAEAARIRDRVRGAREEYKRHSKVPDWNQLKTLVAEPINE
                     LRDRIAEEVRRRESPDALVPIDRDPVPPQFAEGVRRYYERLGSGR"
     gene            617..2983
                     /locus_tag="VHS97_17310"
     CDS             617..2983
                     /locus_tag="VHS97_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450017.1"
                     /translation="MTMPSLIWGSPQWMVAALVILGLAAAIIVWSYARATTKRSVKVA
                     AAILKVAGFSALALSLVDPLLTGTRPRRGANAFVILADNSQSLLIRDDQSSRTRGEWM
                     RDVLRQDTPWRTRLGQDFDVRGYVFDTHLRAVDGFDDLQFDGTGTSLTTSLGALSKRF
                     QGLPLAGVLLFTDGLKTDTTDVDWSGLPPIYPVVPPSPGVAKDLGLSNISLNQTNFES
                     APAVIRAEVAEAGFQGESIVAAVFDQTTGKELERKEMIANGDGKPLSFRFQFRPEKKG
                     VSFYQVRTFKASGGSSGESADGDNASHAEQTKENNSRLVVIDQGGGPYRVLYVSGRPD
                     WEFKFLRRAVDADDQIELVGLLRIAKRQPKFDFRNPRAQSTSPLFDGFDHPDAETAER
                     PDEPVFLRLGIKPDGVELRDGFPKTADDLYRYHAIVIDDLEAGFFTPDQQALVRKFVS
                     QRGGGLLMLGGPDSFASGKYDRTPIGELLPVYLNRPDTGPPEGDYRWALTQEGKLEPW
                     VRLRKTESEENQRLEAMTSFQTISHASGIKPGAVVLANVSDAAGATAPALVAHQFGKG
                     HVGAILVGGLWHWGMRRESSAESDLEKSWRQTVRWLVGDVPSRVEVSVRQKAESSSPA
                     IEVTVRVRDAEYLPLDNAKVSLKITQPGGEVLPLEAEPDGRDPGSYAATYVTKLPGPY
                     RILAAATAPDGSAVGEREAGWAAQPAADEFARLKPDREFLQSIASKTDGKLVDGEQLS
                     SFVGGLLSRSAPITEPWTSPLWHHPLYFLVAIACLAAEWGLRRVNGLA"
     gene            3059..4042
                     /locus_tag="VHS97_17315"
     CDS             3059..4042
                     /locus_tag="VHS97_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450018.1"
                     /translation="MKALLAFCLCLSAARAEDRPCVVIVVGAPGSPEYTGQFREAADH
                     WESAAVKAAAEAIRIGQSTQGAVTDHERLRSVLDEKAKVSSEPLWIVLIGHGTYDGRE
                     GKFNLRGPDVTDVELSDWLLPFKRPVVIINCASASGPFINRLSGNNRLVVTATKSGYE
                     MNFARFGQYISEVIADERADIDKDGQVSLLEAFLTGSSRVDEYYRTHSQLASEHALLD
                     DNGDRLGTPANWFHGIRATKRAKDGATLDGTRAHQLHLIPSNRERGIPTEIRQRRDQL
                     ELSIAALRDLKDKLGEEAYYGQLEKLMVELARLYRSVPAASPSPGEPGHVH"
     gene            complement(4086..5435)
                     /locus_tag="VHS97_17320"
     CDS             complement(4086..5435)
                     /locus_tag="VHS97_17320"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX3450019.1"
                     /translation="MAVELDQDPDHVQPPGEHAALSSASGRIVSVDTLRGLTILLMIF
                     VNDLGPAAPSWMHHIQPPNADGMTLADVVFPAFLFIVGVSIPLALEPWSLAGTQRWRQ
                     IGHILSRTAGLLSMGLIQLNGERDRTLGGPVWELLAFTSLICAWCVVPQDSGMRRTLL
                     VILKTLGFIGLVVLVAVFRGKPQPVDLPFWGHVERWVWFRTEWWGILGLIGWAYLTVA
                     LLWLILGRRREWLMGALGILILLHLAMQHAGLFSRLESKPWLGWSMPMFKTLASGIDL
                     INSYVGLGEATGSLAAISMAGCLLGTILRRDSDVAMPRDRLAWGMTFTIGLLIAGFVT
                     DTFEGINKISATATWCLWSAALTCAVWMLLYLVLDISGIRGWAILVRPAGANPLLAYF
                     LHPIVVELIGVLGLDNQLLNYKGSSDPSIAVAGSLGMAVCVCVATGLLARLGLRVRL"
     gene            complement(5590..>7426)
                     /locus_tag="VHS97_17325"
     CDS             complement(5590..>7426)
                     /locus_tag="VHS97_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HEX3450020.1"
                     /translation="SALCVPGNHDLKLVRKLRGKNVQITHGLAETLAEIDAVPVDIRT
                     SFCTSLADFLDGLVSHYVLDDGKLVVAHAGMKEEMQGRGSGKVRDFALYGETTGETDE
                     FGLPVRFDWASEYRGQAMVVYGHTPVPQTDWLNRTVNIDTGCVFGGKLTALRYPEKEF
                     VSVSAARTYCEPARPFLPAEESAPALSAQQVHDEVLDADDVLGKRIIPTRLWGNVTIR
                     AENSAAALEVMSRFAADPRWLIYLPPTMSPCATSQVTGLLEHPAEAFAYFRNEGIPQV
                     VCQEKHMGSRAVVVVCRDEEAAQERFGVTTGESGIVTTRTGRRFFSDRDLERRFLDRV
                     RVALTATALWTKLDTAWVCLDCELMPWSANAQELLRSQYAAVGAAGSASLPRAVASLE
                     EARLRLAGEQRAKLAEVESRFQRGEQDIDRFVAAYRQYCWPVETLSDLKLAPFHLLAT
                     EGHVHTNRDHHWHMEILAEICRADPELLLATRYRSVDVTDSASQEAGTDWWMELTGQG
                     GEGMVVKPLAFIHKGRRGLSQPAVKCRGREYLRIIYGPGYTSDENLSRLRSRGLGRKR
                     ALALGEFALGIEGLERFVRREPLRRVHECVFGVLALESEPVDPRL"
BASE COUNT         1524 a         2297 c         2276 g         1329 t
ORIGIN      
        1 aacaaggcga agaatccggc ccaggacaac caggcgaacg cggacagcaa gcgcaacaag
       61 gtcaggaggg agagcaggga caacaaggcc agcagggaca acaaggtcag caaggacaac
      121 aaggtcagca aggacaacag ggccaaggcc aatcacaggc tggcaacccc ggccaaaggg
      181 gccaggggca acagcaaggc cggcaggcga attcgttgcg ggggggaggc ggcgcggatc
      241 gtcaaggcgg ccccgatcgc cagcttggtg gaggccccgg cggaccgatc accggcgaag
      301 gattccggca gtggtctgat cggatgcgcg acgtcgagga actgctcgac gatccggaaa
      361 tgcgggcaga ggcagcgcgg attcgcgatc gcgttcgtgg agcccgcgag gaatacaaac
      421 gacattcgaa ggtgcccgac tggaatcagt tgaaaaccct tgtggccgag ccaatcaatg
      481 agctgcgcga tcggatcgcc gaagaggttc gccgccgtga gtcgcccgac gcgctggtcc
      541 cgatcgacag agatcccgtg ccgccgcagt tcgccgaagg tgtgcgccga tattatgaac
      601 gattggggag cggtcgatga ccatgccttc ccttatctgg ggatctccgc aatggatggt
      661 cgcggcactc gttattctgg gcctcgcggc cgcgatcatt gtgtggagct acgcacgggc
      721 gacaacaaag cggtcggtca aggtcgccgc ggccatcctc aaagtggctg gtttctcggc
      781 gctggcacta agcttggtcg atccactctt gacggggacg cggcctcgac gcggggcaaa
      841 cgcctttgtg atcctcgccg ataatagcca gagtttgctc atccgagacg accagtccag
      901 ccgcacccgg ggcgaatgga tgcgcgacgt tctccggcag gataccccct ggaggacgcg
      961 gcttggccaa gacttcgacg tgcgcggcta cgtcttcgat actcatttgc gcgccgtcga
     1021 tggattcgac gacctacaat tcgacgggac aggtacttcg ctgacgacgt ctctgggcgc
     1081 actctcgaag cgattccaag gcttgccttt agccggcgtg ctgctgttca cggatgggct
     1141 gaagaccgac acgactgatg tagattggtc gggcttgccc ccaatttatc ctgtcgtgcc
     1201 cccgtcgccc ggggtggcga aggatctggg tttgagcaat atctcgctca atcagacaaa
     1261 tttcgagtcg gcccccgcgg tgatccgggc cgaggttgcc gaggccggtt ttcagggcga
     1321 atcgatcgtc gccgccgttt tcgatcagac aaccggcaaa gaactggaac ggaaggaaat
     1381 gatcgccaac ggagacggca agcccctgag ctttcgattt cagtttcgcc ccgagaaaaa
     1441 gggtgtgagt ttctatcaag tccgaacgtt caaagcctcg ggcgggagca gcggagaatc
     1501 ggccgacggc gacaacgctt cgcacgctga gcagacgaag gaaaacaaca gccggctggt
     1561 cgtcatcgat caaggaggcg gcccttatcg tgtgctctac gtaagtggac gcccggactg
     1621 ggaattcaag ttcctccgtc gcgcggtcga tgccgatgat cagatcgagc ttgtcggcct
     1681 gttgcgaatc gccaagcgtc agcccaagtt cgactttcgt aacccgcgtg cccaatcgac
     1741 cagcccgctc ttcgatggtt tcgatcatcc cgacgcggaa acagccgagc ggcccgacga
     1801 gccggtcttt ttgcgcctgg ggattaaacc cgacggcgtc gaactgcgtg atggctttcc
     1861 caagacggcc gacgacttgt accgttatca cgcgatcgta attgacgatc tcgaggccgg
     1921 attcttcacg ccggaccagc aggctctcgt acgcaagttt gtcagccagc ggggtggcgg
     1981 gttacttatg ctcggaggtc ctgattcgtt tgcgagcggc aagtacgatc gcactccgat
     2041 cggtgaactg ttgcccgtct acctcaaccg gcccgatact gggccgccgg agggggacta
     2101 tcgttgggct ctcacgcaag aaggcaagct ggaaccttgg gtcagactgc ggaagacaga
     2161 gagtgaagag aaccagcggc tggaagccat gacttcattt caaacaatca gccatgccag
     2221 tggaataaaa cccggtgccg tcgttctggc caacgtgagc gacgccgccg gtgcgaccgc
     2281 tccggctctt gtggcccatc aatttggcaa agggcatgtc ggcgccatac tggttggcgg
     2341 tctctggcat tggggcatga ggcgcgaaag ttccgccgaa agcgacctcg aaaaatcgtg
     2401 gcgccaaacc gtccgatggt tggtcggcga cgtccccagc cgggtcgagg tgtccgtgcg
     2461 ccagaaggcc gaatcgtcga gccccgccat cgaggttact gtgcgcgtgc gcgacgctga
     2521 gtatctccca ctcgacaacg cgaaagtgtc gctcaaaatc acacagcccg gcggagaagt
     2581 cctccctctt gaagccgagc ccgacggccg cgatccgggc agttacgcgg caacgtacgt
     2641 caccaagctg ccagggcctt atcgcatcct cgccgccgcg acggctcccg acggcagtgc
     2701 tgtcggcgaa cgagaagcgg gctgggcggc tcagccggcc gccgatgaat tcgctcggct
     2761 gaaacccgac cgcgagttcc tccaatcgat cgcgtccaag acagatggca agcttgtcga
     2821 tggcgagcaa ctctcctcat tcgttggcgg cctcctttct cggagcgcgc ctatcaccga
     2881 accatggaca tcccccttat ggcatcaccc cctttatttc ctggtcgcga tcgcttgcct
     2941 ggctgctgaa tggggcctgc gtcgagtcaa tgggttggcc tgacgccaag tttgactgag
     3001 cggcgggcgg tcctgacgac ctgaccttct gtatgctacg attaattgga ttgaaaacat
     3061 gaaggcattg ctcgcttttt gtctttgcct gtcggcggct cgggccgagg atcggccgtg
     3121 tgtcgtcatc gtcgtcggcg cgccgggctc acctgaatac acgggccaat tccgcgaggc
     3181 ggctgatcac tgggaaagcg ccgccgttaa agcggccgct gaagcgattc ggatcggcca
     3241 gtcaacccaa ggggccgtga ctgaccatga acgactgcgc tccgttctgg acgaaaaggc
     3301 gaaggtcagt tctgagcctc tctggatcgt gctgatcggg cacgggactt acgatgggcg
     3361 cgagggcaag tttaacctgc gcgggccgga cgtgaccgac gtcgaacttt cggattggct
     3421 gttgcccttc aagcgacccg tggtgatcat caactgtgcg tcggcaagtg gcccttttat
     3481 caatcgcctc tcgggcaaca accgcctggt ggttacagca accaaaagcg gctacgagat
     3541 gaatttcgct cgattcggcc agtatatttc cgaggtgatc gccgacgagc gggccgacat
     3601 cgacaaggac ggccaagtat cgctcttgga ggcattcctg accggcagca gccgtgtgga
     3661 tgaatattat cgcactcatt cacaactggc cagcgagcat gcactcctgg atgacaatgg
     3721 cgaccggttg ggaacgccgg caaactggtt tcatggtatt cgcgcgacca aacgagcaaa
     3781 ggacggcgcg acgctggatg gcacacgagc gcatcaattg catctcattc ccagtaatcg
     3841 cgagcgggga attcctaccg agatacgtca gcggcgcgat cagctcgagt tatccatcgc
     3901 cgcactgcgg gatctaaagg acaagctagg cgaggaagcg tactacggcc agctcgagaa
     3961 gctgatggtc gagctggccc gtctgtatcg cagtgtgcca gccgcttccc cgagcccggg
     4021 ggaaccgggg catgttcatt gattgccgcc aagcggagat tcgggctcag tggacgctgc
     4081 cccgatcaca gtcgcactcg cagccccagt cgggccagga gtccggtcgc gacgcatacg
     4141 caaacagcca tgccgagcga gccggccacg gcgattgaag ggtcgctcga gcccttatag
     4201 ttgagtaact gattgtcgag accgagcact ccgatcaatt cgacaacgat tgggtgaagg
     4261 aaataggcga ggagcgggtt cgcgccggcc gggcgcacga gaatggccca cccgcggatt
     4321 ccacttatat ccaacacaag ataaaggagc atccagactg cgcacgtcaa agcggccgac
     4381 cagaggcacc atgtggccgt ggccgaaatc ttgttaatcc cctcgaaggt gtctgttacg
     4441 aagccggcga tcaaaagtcc gatcgtgaac gtcatgcccc acgccagccg atcccgcggc
     4501 atcgcgacgt cgctgtcacg tcgcaggatc gtgcccagca ggcagccggc catcgagatc
     4561 gcggccagcg aaccggtggc ttctcccaga ccgacataac tgttgatcag atcgattccg
     4621 cttgccagag tcttgaacat cggcatcgac caaccaagcc agggcttgct ctccagccgg
     4681 ctgaaaagac cggcatgttg cattgcgagg tggagcagaa tcaagatccc aagcgcgccc
     4741 atcagccatt cccgcctgcg acccaagatc agccataaga gcgccacagt cagataagcc
     4801 cagccgatca agccgagaat cccccaccat tccgtacgaa accaaaccca tcgttcgacg
     4861 tggccccaga atggtagatc gaccggctgt ggcttaccgc gaaagaccgc taccagcaca
     4921 accagaccga taaagcctaa agttttgagg atgacaagga gagttcgtct catgcccgaa
     4981 tcttgcggaa cgacgcacca ggcacagatc aaactcgtaa acgcaagcag ctcccagacc
     5041 ggtccgccca gcgtgcggtc acgttcgcca ttgagctgga tcaagcccat cgagaggagg
     5101 cccgcggtgc gcgacaggat gtgaccgatc tgcctccaac gctgcgtgcc cgccaaactc
     5161 cacggctcga gcgccagcgg tatcgacaca ccgacaataa acaggaatgc agggaaaacg
     5221 acgtcggcga gagtcatgcc gtcggcgttc ggtggctgga tgtggtgcat ccaggagggc
     5281 gcggcgggac cgagatcgtt gacgaagatc atcaacaaga tcgtcagccc gcgcagtgtg
     5341 tcgacggaga cgatgcggcc actcgcggaa gacagtgccg cgtgctcccc gggtggttgc
     5401 acatgatcgg ggtcctggtc gagctccact gccatgttgc cgttcctcaa gattacgata
     5461 cctgcgtgtc catggatcca ggacgatgga gttcgtccat tggccccact ctaacaccgt
     5521 tttcgccccg gcgccactgg ccagccatcg gatccaaggg agggcgaaca tgcgttagtc
     5581 ctcaatgtct cacaacctcg ggtccaccgg ttcgctttca agggccagca caccgaagac
     5641 acactcgtgt acccgccgta gcggctctct ccgaacgaac cgctccagtc cctcgatgcc
     5701 gagcgcgaat tcaccgaggg cgagtgctcg cttacggccc agcccacgcg accgcagacg
     5761 actgaggttt tcgtcggacg tgtagccggg accatagatg atccgcaggt actcacgccc
     5821 gcggcacttc acagcaggct gactgaggcc ccgccgaccc ttgtggatga aggccaatgg
     5881 cttgacaacc attccctcgc ccccctgccc cgtcagttcc atccaccagt cggtccccgc
     5941 ctcctggctc gcggaatcgg tcacatcgac ggatctgtac cgggttgcta gcaatagctc
     6001 gggatcggcg cggcagatct cggccaaaat ctccatgtgc caatggtggt ccctgttcgt
     6061 gtggacgtgc ccctcggtgg cgagcaagtg gaacggcgcc agcttgaggt cggagagcgt
     6121 ctccacaggc cagcagtact gccggtacgc ggcgacgaac cgatcgatgt cctgctcccc
     6181 gcgctggaat cgggactcga cttcggcaag cttcgcccgc tgttcgcccg ccaggcgaag
     6241 acgggcctct tctagcgacg cgaccgcacg gggcagcgac gccgacccgg ctgctcccac
     6301 tgcggcatac tgcgaccgca gcagttcctg ggcattggcc gaccagggca tcaattcaca
     6361 gtcgaggcat acccatgccg tgtcgagctt ggtccagagg gcagtcgcgg tgagcgctac
     6421 acgcacccgg tcgaggaacc gccgctccag gtcccggtcg ctaaagaacc ggcgccccgt
     6481 ccgggttgtg acgatcccgg actccccggt ggtcacgccg aaccgttcct gtgcggcctc
     6541 ctcgtcgcgg caaacaacaa ccactgcccg ggatcccatg tgcttctcct ggcagacgac
     6601 ctgtgggatg ccctcgttgc ggaaatacgc gaacgcctcg gccggatgct cgagcagccc
     6661 agtgacctgg ctcgtcgcgc agggcgacat ggtgggcggc aggtagatca gccacctggg
     6721 gtcagcggca aaccggctca tcacctcgag ggccgccgcg gaattctctg cacggatggt
     6781 gacgttgccc cagagtcggg tcgggatgat ccgcttgccc agcacgtcgt cggcatcgag
     6841 cacctcatcg tggacctgct gggctgaaag ggcgggtgca ctttcttcgg ccggcaggaa
     6901 cggtcgagcc ggctcgcaat acgttcgggc ggcggagacc gagacaaact ctttttccgg
     6961 gtagcgcaag gcggtgagct tgccgccgaa gacgcaacca gtgtcgatgt tgaccgtccg
     7021 gttcagccaa tcggtctgcg gtaccggtgt atgaccgtag acgaccatcg cttgcccccg
     7081 atattccgac gcccagtcaa agcggacggg gagcccgaac tcgtcagtct cgccggtggt
     7141 ctcgccgtag agtgcgaaat cccgcacctt gcccgagccc ctcccctgca tctcctcctt
     7201 catcccggcg tgcgcgacca cgagcttacc atcgtcaagg acgtagtggc tgactaggcc
     7261 gtcgaggaag tccgccagac ttgtgcaaaa actcgtccgt atgtcgaccg gcactgcgtc
     7321 gatctcggcc agggtttcgg cgaggccgtg cgtaatctgg acgtttttgc cccggagctt
     7381 gcgcacaagc ttcaagtcat ggttgcctgg cacacacaag gccgag
//
LOCUS       DATAQL010001002         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2698488, whole genome shotgun sequence.
ACCESSION   DATAQL010001002 DATAQL010000000
VERSION     DATAQL010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698488"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..794)
                     /locus_tag="VHS97_17330"
     CDS             complement(414..794)
                     /locus_tag="VHS97_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450021.1"
                     /translation="MISAERGRILIIMLIAVVALAVGETLLSKGMKQAGRGGDGWAQL
                     AAGVIRSGWIWSGLILLIIHVGLYMLALKGADLSLALPLTAASYPLAALLARFYLREE
                     VGTSRWIGTLVITAGVAIVALGDG"
     gene            complement(804..2108)
                     /locus_tag="VHS97_17335"
     CDS             complement(804..2108)
                     /locus_tag="VHS97_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX3450022.1"
                     /translation="MTAPSPMDKLGDALRRNTLVRIIEQFGHEHSDSHLKDLVIDEAG
                     PGRIIKVLGRRVVNFGSDSFLGLDRDPRVQEAVRRGLRRWGTHNGSSRAFASVRSNID
                     AEQKLADWLGVESVLIFPSVMLANLGAIPGLVGRQDAVVLDEHAHSSMQEAAKIARAN
                     GAKVTTFAHNDPLALEKTLESLQPYRCALVCIDGVYSMSGMVPPMAELNQVARACDAV
                     LYIDDAHGTGVLGEQGRGTVLEALGSYDNTFVVGSLSKAFSCAGGFIGCTEPFHRLLK
                     IRSSPYVFGGPVVPCYLEAIGAVIDILRSGEYEYLRARLDSHIERLTRGLSDLGLTIM
                     GGATPIVSVLIGDEADTLRAGKFLFDRGYFVQSVLFPAVPYHCGVLRVQCNANHDPAS
                     IDGLIAAFADFALTLPLPAHSEHLAAVAVHDAHSNGAPFLER"
BASE COUNT          528 a          772 c          767 g          458 t
ORIGIN      
        1 gctcgcgccg cggcacgcgg gccacgatcc tctcccttga acttgacatt tgcgagggat
       61 tcctcataaa taggttttgc gaggctcgcc tcatgttacc acaggcggtc gcgatatcca
      121 tctcccggga cgagcgatcc atcacgcaac gctcgggaac acggctgcga gatcgtcatc
      181 gccgaggacg tgacgtcgag cttgtccgcc gagctgcacg atttctcgat gcagcacatc
      241 ctgccgcata tcgcggtcat ctcatcctcg gccgccattt tgttcaagga agattaatgg
      301 tgccattctg tgccattttg tcccgcgccc gagacctctt gataatgcca caagtcccgg
      361 cccggcgatg cgttgcgatc gcggacggca tgtccatcgc ctggggcatc cagctatccg
      421 tcgcccaagg ccacgattgc gacgcctgcc gtgatcacga gtgtgccgat ccagcgagac
      481 gttccgacct cttcgcgcag atagaaccgc gccaggagag ccgctagcgg atacgacgcc
      541 gcggtgagtg ggagtgccaa actgaggtcg gcccctttga gcgccagcat ataaagcccg
      601 acgtggatga tcaaaaggat caggcccgac cagatccagc cgctacggat cacgccagcg
      661 gccagttgcg cccagccgtc gccgccacga ccggcctgtt tcatgccttt ggagagcagc
      721 gtttccccga cagcaagcgc gaccaccgcg atcagcatga tgatcaggat ccgaccgcgc
      781 tcggcggata tcacagtcgt acctcacctc tctaaaaaag gcgcaccgtt tgagtgggcg
      841 tcgtgcacgg caaccgcagc cagatgttcg gaatgggccg gcagggggag ggtaagggcg
      901 aaatcggcaa aggcagcgat caagccgtcg atcgacgcag gatcgtggtt ggcgttgcac
      961 tggactcgga ggacgccgca gtgataggga acggccggga agagtacgga ctgaacgaaa
     1021 tacccacgat caaagaggaa cttgccggcg cgcagggtgt cggcctcgtc accgatcaag
     1081 accgacacga tcggcgttgc cccccccatg atcgtcaagc caaggtcgga aagtccacgt
     1141 gtgagccgct cgatgtgcga gtccagacgc gcccggaggt attcgtattc gccggaccgg
     1201 aggatgtcga tgaccgctcc gatcgcttcc aaatagcagg ggacaaccgg gccaccgaag
     1261 acataagggc ttgaacggat cttgagcagc cgatggaatg gttcggtaca gccgataaag
     1321 ccgcctgcac acgaaaacgc cttggaaagc gatccgacca cgaacgtgtt gtcgtagctg
     1381 ccgagggctt cgaggaccgt acccctcccc tgctctccca gaactcccgt gccgtgggca
     1441 tcgtcgatat agagcacggc atcacacgcc cgggccactt ggttgagttc ggccatcggc
     1501 gggaccatac cgctcatgct gtaaacgccg tcgatacaaa ccagggcaca acgatacggt
     1561 tgcaagctct ccagggtttt ttcgagtgcc agcggatcgt tatgggcaaa ggtcgtcacc
     1621 ttcgcgccat tggcgcgggc gatcttggcg gcttcctgca tcgagctatg agcatgttcg
     1681 tcaagtacga ccgcgtcctg gcggccgacc agaccaggga tcgctcccag attggcaagc
     1741 atcaccgacg ggaagatgag caccgactcc acgcccagcc agtcggcgag cttctgctcg
     1801 gcatcgatgt tgctgcggac gctcgcaaaa gcccgcgacg agccgttgtg cgttccccaa
     1861 cgcctgaggc cacggcggac ggcctcctgg acgcgggggt ctcgatccag gccgaggaaa
     1921 ctgtccgagc caaaattgac aacccggcgg ccgagcacct tgatgatccg gcccgggcct
     1981 gcttcgtcga tcacgaggtc tttaaggtgt gaatcggaat gctcgtggcc gaactgctcg
     2041 atgatcctga cgagtgtatt tcggcgtagg gcgtccccca gtttgtccat tggggaaggc
     2101 gcggtcatga cgtctcctta cgtagcggtc tggtccgtta agccactgat cgatcccgtt
     2161 ggccgcatgc gcaaagccaa ggaaatcgcg gacgagagcc gcctccatcc gatagagtgc
     2221 gattgaaaac accatgatcc taaagataga ctcatgcgcc aaggtcggtc gcgcggatcg
     2281 atgaggaacg catcacccga aatatccgcg gcgtgcggtg gtcgtcagcg atcacgctaa
     2341 agcgcattca ctgccttttt ttactggata caggattcgg aagccaggcg ctgcccaaag
     2401 gaaggccctc cacggtgacg cagcaaagga ttgagcgatc aagggcgagt gccaggccgc
     2461 gggccgaatc ggtacttgcc gcgagtgtcg tgggatatcg cctgttccgg atgtcgcctg
     2521 gcgac
//
LOCUS       DATAQL010001003         7568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_863180, whole genome shotgun sequence.
ACCESSION   DATAQL010001003 DATAQL010000000
VERSION     DATAQL010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863180"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VHS97_17340"
     CDS             <1..892
                     /locus_tag="VHS97_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX3450023.1"
                     /translation="SMDEASARRASRAAYDRIAALNTILSDYDPESELSKLARTAGGP
                     PVAVSTDLFDVLQRSKAVYDLSGGAFDVTIAPVGRLWRRARRDRKLPDPGRLAEARLL
                     VGSNQMVLDTKARTIQLKKPGMKLDVGGIAKGYAAQAALDVLKGAGVSRALVGGAGDI
                     VVGDPPVGERGWKIAVAPLEPGKPDPHRVLLLANAAVSTAGDAERYVVIDGHRYSHII
                     DPATGVGVEDRASVTVVAPDGATADALETTVYILGPERGIKLVDETPGAAAIFVRSTP
                     DGIKTFESSRFKQVPTVPAD"
     gene            complement(861..2201)
                     /gene="aroA"
                     /locus_tag="VHS97_17345"
     CDS             complement(861..2201)
                     /gene="aroA"
                     /locus_tag="VHS97_17345"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3450024.1"
                     /translation="MTTTGYPSEILIELWTSPPPCASAHVPGSKSLTNRALVVAALAQ
                     GPSILRGALDCEDTQVMVAALRNIGIDVTHDPASALIKVEGCSGVIPSHQASLAVANS
                     GTSLRFLTAILSTGQGTFHLDGSPRMRERPISDLLVALNGLGAKAKSDHGSGCPPVTI
                     EANGLDWGSVFVRGDVSSQFLSGLLMALPAARQPTTVEVEGVLVSQPYVAMTLAVMES
                     FGVTAANRDFRRFDVPAAAYFGREYSIEPDASAASYFFALAAVTGGAVTVEGLGTSSI
                     QGDMNFVDVLEQMGCTVKREPAKTTVFAGPLHAVDVDMNAFSDTVMTLAVVALFARGV
                     TRIRNVAHIRHKETDRIAALATELRKLGARVDEFADGLVIHPPQPDRLRGARIHTYND
                     HRMAMSFALAGLLIPGVTIVDPGCVKKTYPDFWTDLETLRTSSRLNRPEPSAPA"
     gene            complement(2238..2558)
                     /locus_tag="VHS97_17350"
     CDS             complement(2238..2558)
                     /locus_tag="VHS97_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX3450025.1"
                     /translation="MIATDPRDGGVVVHVLAHPGSKRDAILGERAGLLRVAVTAPPEK
                     GKANTAIQTVLAEAIACSRSQITLVSGETARRKKFLVEGIPPDELNRRLAILVNPEPR
                     RQES"
     gene            complement(2555..3256)
                     /locus_tag="VHS97_17355"
     CDS             complement(2555..3256)
                     /locus_tag="VHS97_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX3450026.1"
                     /translation="MNVERIRYNLEAVERRITDAARRVGRPRDSVRLIAVTKKSPPEC
                     IKALLNCGVRDCGENYPQELWRKHDELGDLTGSIHWHLIGHLQTNKVKKTLPMVRVIH
                     SVDSLKLLRALDDAVPALNNPPSVCLQVNTSNEPSKHGWTAEVILRDATAIATCRAIP
                     IVGLMTIAALGTTAETARGSFVRLRELRDLLRRETDLPLVELSMGMSNDFETAIEEGA
                     TLVRVGSALFEGVDT"
     gene            3396..3602
                     /locus_tag="VHS97_17360"
     CDS             3396..3602
                     /locus_tag="VHS97_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450027.1"
                     /translation="MKPFLMIVGFLAAILIVSQLVMGLLILQGQATMRTAHQHSGYLT
                     VAVSLIYIAWSLTVIAAAPKRNGP"
     gene            complement(3655..4749)
                     /locus_tag="VHS97_17365"
     CDS             complement(3655..4749)
                     /locus_tag="VHS97_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450028.1"
                     /translation="MKTLLEITTFNNNWPSSWDDPITKLIQTTFRFSRADGTPVTHFD
                     EPTRSKAFVGTSFDVLGGSTIARIAGDFVSRYAKKPIGSRNESSDAGSRSIRALAVLR
                     PGWPDGSDFLAIDHRRADASCRLELFAAGRSWLGPTWMVVGESGPTSHSKRPSLMPDS
                     SESLAEWSYSAGQARITHSAVLLRESKLALLSALIERRSPGDSNLVLSLSLPSPVTAT
                     PIVDSRSIVLTTPKKPGSAQVLPIGLPSLSYPSDRGSFQVHDGSLALSHSPTGRRTWL
                     PLLVSWDSRRHRTDPRWRVLSVSEKSRNVPPDRAFAARVSWGRNETFVIYRSLASPAP
                     RAFLGHQTSARFLVARFTLDGVVEPILKVD"
     gene            complement(5561..7294)
                     /locus_tag="VHS97_17370"
     CDS             complement(5561..7294)
                     /locus_tag="VHS97_17370"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX3450029.1"
                     /translation="MFRTRLRWMLLVSAAVGSIASGQEARPDSYDIVVRGGKIVDGTG
                     NPWFQGDVAIRGDRIAAVGSLGPQVKARHTIDATNLIVAPGFIDMHSHSDMTLLEDGS
                     AQSKIRQGVTTEVLGEDTSAGPAKGKRPPRSVTFGGIPLTWTTLGGYFDALERQGVAV
                     NVASYVGLGTLLECAQGDSLVRPDRSRLEEMKVLLDEAMSDGAIGLATMLASPRELAV
                     TTQDLVELCQVVKRHGGLFSSHIRNEGSDVFDAVVEAITVARSAHVPVDIIHLKIADR
                     SLWGRMKEIVALIDQARRDGVNVQANVYPYTRGNNDLVSIIPPWAHEGGKPQLIARLR
                     DRSLRDRLKRDIRTGLPGWYNHYTAVGGDWSRMLVSGHLSLANQSFEGQTMDRIIEAK
                     SAGVAPHPDALDVLFDFLIDENASISTIYAHHSEDDMNFALVQPWCSVGSDGSALAIV
                     GPLRRGHPHPRNFGTFPRVLGEYVRNRQLLRLEDAVRKMTSLNAAKVGLSDRGLLKPG
                     QYADIAIFDPATVIDHSTYLEPFQYNSGINSVIVNGKLVLENGQSGPARPGRALRHGR
                     SVPPPDHRTSH"
     gene            complement(7363..>7568)
                     /locus_tag="VHS97_17375"
     CDS             complement(7363..>7568)
                     /locus_tag="VHS97_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450030.1"
                     /translation="PAGASGDLGGADHIDVEWQMRAVLLDGATRENADFAKFDGVVDL
                     GPGEFFIPKLFERTAGHCNYLNG"
BASE COUNT         1512 a         2194 c         2328 g         1534 t
ORIGIN      
        1 ctcgatggac gaggcctccg ccagacgcgc ctcccgggcg gcctacgatc gtatcgctgc
       61 tctcaacacc atcttaagcg actacgatcc cgaaagcgag ctatcaaaac tggcccggac
      121 cgcgggcggg ccgccagttg ctgtgagcac cgaccttttc gatgtattgc agcgctccaa
      181 ggccgtatac gacctttcag gcggtgcgtt cgatgtaacg atcgccccgg ttggccggct
      241 ttggcgccgg gcccgtcgcg atcgcaaatt gccagaccca ggccgacttg ccgaggcacg
      301 gcttttggtc ggctccaacc agatggtgct cgacaccaag gcaaggacca ttcagctcaa
      361 aaagccgggg atgaaactcg acgtgggggg catcgccaag ggttatgcgg ctcaggcagc
      421 cttggacgtg ctcaaaggcg cgggagtttc gcgggccttg gtcggcggtg caggggacat
      481 cgtcgttggc gatccgcccg tcggtgaacg cggctggaag atcgccgtcg ccccgttgga
      541 accgggcaag ccggatcctc atcgggtctt gctcctggcc aacgcggccg tctccaccgc
      601 gggcgatgcc gaacgctatg tcgtgatcga cggccatcgc tattcccaca tcatcgatcc
      661 ggcgaccggg gttggagttg aggatcgcgc gagcgtcacg gtggtcgcac cggacggcgc
      721 gactgccgac gccctcgaga cgacagtcta tatcctgggc cccgagcgcg ggatcaagct
      781 cgtcgatgag acgccgggcg cggctgcgat ttttgtgcgg tcgacgccag acgggatcaa
      841 gacgtttgaa tcgtcacggt tcaagcaggt gccgacggtt ccggccgatt gaggcgagaa
      901 ctcgttcgca gcgtttccaa gtcagtccag aagtcagggt atgttttctt gacacaaccc
      961 gggtcgacga tggtgacccc gggtatcaac aggccggcca aagcgaagga catggccatg
     1021 cggtggtcgt tgtaggtgtg gattcttgct ccgcggaggc gatcgggttg aggtgggtgg
     1081 atcaccaggc catcggcgaa ttcatcgacc cgcgcgccca gcttccgcag ctcggtggcg
     1141 agcgcggcga tgcggtcggt ttccttgtgc cgaatgtggg cgacattgcg gatccgcgtc
     1201 acgcctcggg cgaagagcgc caccactgcc agggtcatca ccgtgtcgga aaatgcgttc
     1261 atatcgacat cgacggcgtg taggggcccg gcgaacacgg tggtcttggc cggctcgcgc
     1321 tttacagtgc agcccatttg ctcgagcacg tcgacgaagt tcatatctcc ctgaatactc
     1381 gaagtgccca gtccctccac ggtgacggca ccccccgtca ccgcggccaa ggcaaagaag
     1441 tagctggccg ccgacgcgtc gggctcgatc gaatactcgc ggccaaagta tgcggccgcc
     1501 ggcacgtcga agcgtcggaa gtcgcggttt gcggcagtca ccccgaacga ctccatcacg
     1561 gccagagtca tggccacata cggctgggat acgagaaccc cctcgacctc aacggtcgta
     1621 ggctgtcttg ccgcgggcaa agccatcaaa aggccgctca gaaactggct cgagacgtct
     1681 cccctcacga atacagagcc ccaatcgagg ccgttggcct cgatggtgac gggggggcaa
     1741 ccgctgccgt ggtcggactt cgccttggct ccgagtccgt tgagcgcaac cagcaaatcg
     1801 gagatcgggc gctcgcgcat gcgtgggctg ccatccagat ggaacgttcc ctggccggtg
     1861 gacaggattg cagtcagaaa gcggaggctt gtccccgaat tggctacggc cagcgatgcc
     1921 tggtgcgagg ggatgacacc cgagcagcct tcgactttga tcagggcgga cgccggatca
     1981 tgcgtgacat cgatgccgat attccgcaag gcggcgacca tcacctgagt atcctcgcaa
     2041 tcgagcgcgc cgcggagaat gctgggtccc tgggccagtg ccgcaaccac gagtgcgcgg
     2101 ttagtaagac ttttggagcc aggaacatgc gccgacgcgc atggaggagg acttgtccaa
     2161 agctcaatga ggatctcgga gggatagccg gtcgtggtca tggcgagacg attcgctctg
     2221 gtgaggattc gtggcgatca gctttcctgc cgccgcggct ctgggttgac gagaatggcg
     2281 agacgacgat tgagctcgtc gggtggtatc ccttcgacca gaaacttctt gcgccgggcg
     2341 gtttcaccgg agaccagggt aatctgactg cgactgcagg caatcgcttc ggccagcacg
     2401 gtttggatgg ccgtattggc tttccctttc tcggggggcg cggtgacggc gacccgcaga
     2461 aggccggcgc gctcgcccag gatcgcgtct cgcttcgagc cggggtgggc gagcacatgc
     2521 acaacgacgc ctccatcccg gggatcggtg gcgatcacgt gtcgaccccc tcgaaaagcg
     2581 ccgagccgac tcgcaccaag gtggctcctt cctcgatggc cgtctcgaaa tcgttggaca
     2641 tccccatcga aagttcgacg agtggcaggt ctgtctcccg ccgcaataag tcgcgcagct
     2701 ctcgcagccg cacgaacgag cctcgggcgg tctcagccgt tgttcccaga gcggcaatgg
     2761 tcatcaagcc gacgatcgga atggctcggc aggtcgcgat tgccgtggca tcgcggagga
     2821 tcacctctgc ggtccagcca tgtttgcttg gttcattgga agtattgacc tggaggcaga
     2881 cgctaggtgg attattgagc gcgggcaccg cgtcgtcgag ggctcgcaac aacttgagag
     2941 agtcgacaga atgaatcacc cttaccatgg gaagcgtttt cttgactttg ttggtctgga
     3001 ggtgcccgat caggtgccag tgaatcgagc ccgtgagatc gccgagttca tcatgtttcc
     3061 gccagagttc ctgcggatag ttctcaccgc aatcgcggac gccgcagttg aggagcgcct
     3121 tgatacattc tggagggctc tttttggtca cggcgatcaa ccgcaccgaa tccctggggc
     3181 gacctactcg tcgcgccgcg tcggtgattc tgcgctcgac tgcttcaaga ttgtaccgga
     3241 ttcgttccac gttcatgaag cggccttcgt gagcttgttt tccgccgaag caattccccg
     3301 agtctgaact gaagcaagga actttccaag ataaaggcga ggaccgactc ttccttcttt
     3361 ccaggctggt ctattttgaa gaacagggca agttgatgaa accgtttctc atgatcgtgg
     3421 gctttttggc ggccattttg attgtgtcgc aactggtgat ggggctcttg atcctgcagg
     3481 gtcaagcaac gatgcggact gcgcaccagc attcaggtta tttaacggtt gccgtgagct
     3541 tgatatacat tgcgtggtcg ttaacggtga tcgccgcggc ccccaagcgg aatgggccgt
     3601 gatgttacgg caaaaaccgg cgcccagttg tcggcctacg tctcgtcaac tgtgtcagtc
     3661 caccttcaag atcggctcga cgactccgtc cagagtgaat cgcgccacca ggaaccgagc
     3721 gcttgtttga tggccaagaa acgcgcgggg agccggtgat gccaggctgc ggtaaatgac
     3781 gaatgtctcg tttcgccccc agcttacgcg cgcagcgaag gcccggtcgg gaggaacgtt
     3841 gcgggatttt tcagaaacgg aaaggactcg ccagcgcgga tctgtgcgat ggcgccgcga
     3901 gtcccatgac accaagaggg gcagccaagt gcggcggcct gttggggagt ggctcaaggc
     3961 cagcgaccca tcgtggacct ggaaggatcc ccgatcggac ggataagaga gcgaagggag
     4021 cccgattggc aggacctgag ccgagccagg tttcttggga gtggttagga cgattgatcg
     4081 gctatcgacg atcggtgtgg ccgtgacagg ggatgggagt gacagactga ggacgaggtt
     4141 cgaatcgccg ggggaacggc gctcgatcag tgccgacagt aacgcaagct tactctcgcg
     4201 gagaagtacc gcggaatgag taattcgagc ttggcctgca ctatacgacc attcggccag
     4261 actttccgac gagtcgggca tcaatgatgg acgttttgaa tgcgaggtcg gcccggattc
     4321 gccgacaacc atccaagtag gtccaagcca cgatcggccc gccgcgaaga gttcgaggcg
     4381 gcaagacgcg tcagcgcgac ggtgatcgat ggccaggaaa tcgctcccgt cgggccatcc
     4441 gggccggagc acggcaagcg cgcgaatcga acggctccct gcgtccgacg attcgttccg
     4501 gctgccgatg ggtttcttgg cgtaccggct tacgaagtcg ccagcgatcc ttgcaattgt
     4561 cgatccgcct aacacatcga acgaggtccc gacgaatgcc ttgctcctcg tgggttcgtc
     4621 gaaatgagtg actggcgttc catccgcccg ggagaatcga aaggtagttt gaatcagctt
     4681 tgtgatcggg tcatcccagg aggatggcca attgttgttg aaggtcgtga tttccaacag
     4741 tgttttcaca caaaggtgcc aatccggtat cggcagacgg atcggagctc ctcggggatc
     4801 caattctcga ctcagccacc tcgtcaatgg ctgcgtgttt ccatggcgtt gccagaattc
     4861 gatcgcttga tggagcgcgg tgagtacacc ttcccgggtg gagcggtttt cattcatagc
     4921 ggctattccg tctaccctcg cgcggatgca gcccttctgg gcgggcgtgc acataaaatc
     4981 gacttcccat ggtaggaacc ggacagcaga cgtggactaa agacatcgaa atccctgctg
     5041 caacgagccg acgtaatccc atgccccaaa tcgcacttat gatgaacaat gattaccgtc
     5101 tcaaccgcaa gaattcgctt ctttaccatg gcgaactgat tctggcactc gatttgctcc
     5161 tgctcatttc atgtgaagcg atgaaacaag atcaacgata agtagaaagc aatcaagctc
     5221 acaggaatca ctttttcaag aagccacccc acttggcgat agtcgcttcg acacacttgg
     5281 agcggctccc cgccaaaggg cgagcgggtt gcctcgcacg accccccgct cgccctttct
     5341 caagacacgt tagaaggcgc atgaaatgcg tcttctgcat cggggccaac cggccagtcg
     5401 aatgggtttt agacgactag gggagaggta gtcgtttgga gcttgttctc tggccggttt
     5461 ttttcatttc gaatcaagag cgtaattcgg ttaccgggat gcattgcaac attgaagatg
     5521 aacagcgata ttttcatagt tgcaatcgtg cgatcatttt tcagtgactc gttctatgat
     5581 ccggcggagg cactgaccgg ccgtgccgca aagcgcggcc cggtcgcgcc ggaccagact
     5641 ggccgttttc caacaccaac tttccgttaa cgatcacaga attgatcccc gagttgtact
     5701 ggaacggttc taggtaagtg gagtgatcga tcaccgtcgc cgggtcaaat atcgcaatgt
     5761 cggcatactg gcccggcttg agcaggccgc ggtcgctgag accgactttt gccgcattga
     5821 gcgacgtcat cttgcgcacg gcgtcttcta gtcgcagaag ctggcggtta cgaacgtact
     5881 cacccagaac tcgggggaat gtaccgaagt tgcgcggatg gggatgaccg cgacggagcg
     5941 gtcccacgat agccagtgcc gaaccgtcgg agccgaccga acaccaaggc tgaacgagcg
     6001 cgaagttcat atcgtcttcc gaatgatggg cgtagatcgt gctgattgag gcgttctcgt
     6061 cgatcaggaa gtcaaagagc acatcgagcg cgtcaggatg aggggcgaca ccggccgact
     6121 ttgcctcgat gattcggtcc atggtctggc cctcaaatga ctggttggcg aggctaaggt
     6181 ggccactgac taacatgcgc gaccagtctc cgccgacggc cgtgtagtgg ttgtaccagc
     6241 cgggcagacc ggtccgaata tcgcgcttca gtcggtcgcg caacgaccgg tctcgcagcc
     6301 gcgcgatcag ctgcggcttt ccgccttcat gtgcccatgg cgggatgatg ctaaccaaat
     6361 cattattccc tctcgtgtat ggatagacgt tggcctggac gttgacgcca tcacgcctcg
     6421 cctggtcgat caaggcgaca atttctttca ttcgtcccca cagtgacctg tcggcgatct
     6481 tgaggtggat gatatcgacc gggacatgtg ccgaccgcgc gacggtaatc gcctcaacaa
     6541 ccgcgtcgaa cacgtccgaa ccctcattgc gaatatggga tgagaataag ccaccatggc
     6601 gtttaactac ctggcagagt tcgaccaggt cttgggttgt gaccgcgagc tcgcgcgggc
     6661 tcgcgagcat cgtggcgagt ccaatcgctc catcgctcat tgcttcgtcg agcagcactt
     6721 tcatctcttc cagacgggat cggtccgggc gaacgagcga gtcgccctgc gcacactcaa
     6781 ggagcgtgcc cagcccaaca tagctggcga cattgaccgc cacgccttgt cgctcaagtg
     6841 cgtcgaagta gccgcctaga gttgtccagg taagcgggat tccgccgaaa gttacggagc
     6901 gcggaggccg cttacccttg gcgggtcccg ccgatgtatc ctcacccaag acttcagtcg
     6961 taactccctg ccggattttg ctttgcgcgg agccgtcttc aagtagcgtc atgtccgaat
     7021 gtgagtgcat gtcgatgaat ccgggggcga cgatcagatt tgttgcgtcg atcgtgtggc
     7081 gggccttgac ctggggccca agcgatccga cagccgcgat ccggtcaccg cggattgcca
     7141 catcgccttg aaaccagggg tttcccgtcc cgtcgacgat cttgcctccg cggacaacga
     7201 tgtcgtaaga atctggccga gcttcctgac cggacgcgat tgagcctacc gccgccgaga
     7261 cgaggagcat ccagcgtagc ctggtgcgaa acatggttaa tgatcctgat tgcaaggtcg
     7321 tgctcgcgct tccagaatag gacgaattcc aattaggttt gtctacccgt tgaggtaatt
     7381 gcaatggcca gccgtgcgct cgaagagttt cggtatgaaa aactcacctg gcccgagatc
     7441 gacgacgccg tcgaacttgg caaagtctgc attctccctt gtggcgccgt cgagcagcac
     7501 ggcccgcatc tgccactcga cgtcgatgtg atctgcccca ccgagatcgc ccgaggcgcc
     7561 ggccggat
//
LOCUS       DATAQL010001004         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4070931, whole genome shotgun sequence.
ACCESSION   DATAQL010001004 DATAQL010000000
VERSION     DATAQL010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4070931"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(436..1457)
                     /locus_tag="VHS97_17380"
                     /pseudo
     CDS             complement(436..1457)
                     /locus_tag="VHS97_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1561..1885
                     /locus_tag="VHS97_17385"
                     /pseudo
     CDS             1561..1885
                     /locus_tag="VHS97_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455796.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          352 a          536 c          592 g          405 t
ORIGIN      
        1 cgccaatttc accggacgga actaaacggg gtcttaaccc actgaaaatg ctggggcacg
       61 gaactaaaat tgctgttagg tacgttcctc tccattgcgc ttcagtgcgt aaggtcgatg
      121 ccaccaaatc gcggagcgct ctagagtcca aacccaatca aggcattgag atgagtatcg
      181 gactgtgatt catatgtgga cgcccccttt ggcaaggcgt ttttgtcgag atgcgtgatc
      241 ggttgctttc atatgtccgg gctgtttgtg cggcatagat tttcgccgct ggccctgatg
      301 gaaatccgcc ggtgaggttc ctgatcaagc tggcgcgctg ttgaagcgca ttgaatcaga
      361 cgggttggcc tcaccattgg ctcgatcgtt acacatcatc gactgccatt gccaattcgg
      421 tttgcccagc cttcctcagg ccgctagttg atagggccgg tacgcttcct ttcgcgtcag
      481 gacgacccac gcgatacgcg ccagtttgtt ggcgagcgcg accgagacga ccctgaacgg
      541 cttcttcacc aagagcttcc tgatccagtt ggccatgggg gttggcttgt ctttcgtgtg
      601 gcgaatgacg gctgtagcgc cgacgacgag caacctgcgc aagtatcggt ctccttgctt
      661 ggagatgccg ccgagctgtg tcttgccccc ggttgaatgc tgttgaggcg tcaagccgag
      721 ccaggcagca aattgtctgc ccgaccggaa ctggtcggga tcggtgacgg tcgcggcgat
      781 ggctgttgct gtgacaatgc caaggccggg gatcgcagcg agcctgcgac tggcctcgct
      841 gtcgacatgc cacgcgtgga gcttgcgatc caacgccgtg atttcttcgg aaagcgtcat
      901 gatctgccgg accaggactt gcagcgtcgt acgggcatag gctggcaggg cgtcctcgtc
      961 ggacagcgac tgctgcgcca gcttcgccag gttggcgatt ccggggttgg tgacgagacc
     1021 gagttccgcc agatgcgctc gcaaagcatt ggcgagcatg gtgcgctggc gaacgagcag
     1081 cgacctggcg cgatgagcca tgagaatgct ctgctgctcg acagtcttta tggatacgaa
     1141 ccgcatcgtc gggcgctgca ccgcttcgca aattgcttcg gcatctaaag catcgctctt
     1201 gccgcgcttg acgaatgcct tcacgtagga gggcggaata aggcggacct cgtgccccat
     1261 ggcggcgagg gtgcgagcca gtgatgggcc gttccacacg cctccatccc gatcaagcac
     1321 ggcggcagct ttgagaggaa ggggagcatc tgtttgcgat gcagccgctt tgtcagcaaa
     1381 gtggcgccca tctcgtcgac gccgtgggct tgaaacacgc ttttcgccaa atccagtcca
     1441 atcgttctga tcgtcattgt aaacgctccc tatcccgctt cgggccaatc ggcatcatag
     1501 ccgccggccg gtgcgggggc gtccacatca tcaagccgaa tcgggagcgg ggcttgaatc
     1561 atggcgaagg aattttggct gagcgaggtg cagtggacgg cgattgagcc gcttcttccg
     1621 aagaaccaac ccggcgcccg gcgcgtcgat gatcgaggcg tcatcagcgg ccttgtcacg
     1681 ttctcaaagt tggctgccga tggcaggatt gtccggccgt ctacggccct ccgaccactg
     1741 tctacaatcg gtttcatcgt tgggcgatgc gcggattatg gcgtcggttg ttcgaggcgc
     1801 tggtaaggac tgagcctggt gacgttcagg ccatcgacag cacgacagcc agggcgcacc
     1861 gctcggccgc cggcggaaaa ggggg
//
LOCUS       DATAQL010001005         6725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_101461, whole genome shotgun sequence.
ACCESSION   DATAQL010001005 DATAQL010000000
VERSION     DATAQL010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101461"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1569)
                     /locus_tag="VHS97_17390"
     CDS             complement(85..1569)
                     /locus_tag="VHS97_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450031.1"
                     /translation="MDPIRERIEGLTRRHFFGRAGLSLGTAALSTLLAEAARCDTTPT
                     GGSTSAASLTGGLPGLPHFPPKAKRAIYLHMNGGPSQMDLFDYKPKMGAMYDKDLPES
                     IRNGQRLTTMTSGQSRFPVAPSKYKFAQHGQSGIWMSELLPWMARLADEIALVKTVWT
                     EAINHDPAVTYICTGHQLPGRPSLGSWLSYGLGTMNQNLPAFVVMTASWSSKAAAQAL
                     YNRLWGAGYLPTKHQGVALRSIGDPVLFLSNPQGIDAATRRRALDAINRLNHSEYEQF
                     ADPETQARIAQYEMAFRMQTSVPELTDISNEPKSVLDMYGPDVHKPGSYARCCLLARR
                     LAERDVRFVQIFHRGWDQHANLAGDLPLQCQDVDQATFALVADLKQRGLLDDTLVIWG
                     GEFGRTVYCQGKLSRENYGRDHHPKCFPLWMAGAGIKPGIVHGETDDFSYNVVADPVH
                     IHDLNATILHCLGIDHRRLSLKFQGLDVRLTGVEEHHPVKGILA"
     gene            complement(1592..4738)
                     /locus_tag="VHS97_17395"
     CDS             complement(1592..4738)
                     /locus_tag="VHS97_17395"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3450032.1"
                     /translation="MASCLRNALLLVTLGTLGSAHGEGDPVPSTRDVKPRPVNFARDV
                     RPILSDNCFSCHGPDDKARKAKLRLDTKEGAFAKLESDALAIVPGKPDDSELILRIES
                     DVSDLRMPPKKSGKQLSADQAAVLRRWIEQGAPWSTHWAFDPPKKPALPAVKNTGWPS
                     NEIDRFILARLEAEGLSASPMAAKTNLIRRVTLDLTGLPPTIQEVDAFLADPSREAYE
                     KLVDRLLDSPRYGEQMARVWLDAARYGDTHGLHLDNYREAWPYRDWVIRAFNANKPFD
                     RFIVEQLAGDLLPNPTADQIIATGYNRCHVSTNEGGSIEEEVYVRNIVDQVDTNGTVF
                     LGLTTGCARCHDHKYDPIRAKDYYQLFAFFNNIDGPAMDGNSSKWEPVAQVPNPKQSE
                     ALKSTDSQIAEVQKSIAAEAAIAATAYDAKAESAESEAPGRADYVWIDDALPNGASPQ
                     GDGPWEFVANTDAPDFGGRRALRITAKGLKQRFFDNAARKLKIGKGDTLFADVYIDSS
                     SPPQELMLQWHTKGGWSHRAYWGENLIDWGADGTPARLRIGDLPASKKWVRVEVPVAK
                     LKLAAGTLIDGWAFTQHGGTVYWDKAGIETETPQEGQTFDSLAAWVRVQRASGSTGLP
                     ANVKAIVALDRSKRTETQTNELRTYFVGHAYSKTSGIFEPFRSNLEELERKRKELNDQ
                     IPTTLVFREKAGDPKPAFLLKRGEYDQRGEKVGRAVPAFLPPLPPGAPVNRLGFAQWL
                     IAPEHPLTARVAVNRLWLQVFGTGIVKTAEDFGAQGEPPSHPELLDWLAVQFREDGWD
                     VKRFMKRLVMSATYRQSSRVTPETLAKDPSNRLLARGPRFRLDAEMVRDQALFAGGLL
                     VETVGGPSVKPPQPSGLWEAVAYTDSNTAHFAADTGVEKIHRRSFYTFWKRTAPPPQM
                     TTFDAPSRESCQVRRERTNTPLQALLLMNEPQFIEAARALAERTLHEGGSTADDRLTY
                     MFRLVTARTPEPKDFAELSSALKDLTAHYAKEPEAAKQLISAGATKPDAHFSASELAA
                     WTMVGNVILNLDEAITKG"
     gene            5249..>6725
                     /locus_tag="VHS97_17400"
     CDS             5249..>6725
                     /locus_tag="VHS97_17400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450033.1"
                     /translation="MGFSDAQLLERFVSDHDDAAFEPLMARHGPMVLSVCRGILKDPN
                     DAEDAFQATFLILVKKSAAFRGHVSLGPWLYRVAHRVAIRANVAATKRRAWEREAGQM
                     HGTSSIMGLAQPPDQLQALHEEIARLPEKFRRAVVLCDLELVPQTRAAAELRLSERTL
                     QRRLRQGRERLKARLIRRGLASDDAMLGAVFLREAHSAVPASWSEATIRAVLAGVNQG
                     ISTGVVSAAAEKLTDEVLRIMLVQKLTLGSATLLAAGLIAWGASAALVSLAEEPSKKT
                     IANSHSPVQPKSETPVRQPEPQLLDSTDKISIGGRVLGTDGQPVADAKLYITVMRGYY
                     REPFPAKENAITGPDGRFSFTVSKSTIGDEKIVAAAIAANHGLGWLEVPAAGNRHDVT
                     LRLVNDDMPITGQVVDLEGKPVADATLRVLEISAAPGEDLGRRLEAARGKTEEKSRLE
                     TEDFKWNAINVSRLAIKVTTDADGRFVLTGIGRNRLVRAQLD"
BASE COUNT         1252 a         2015 c         2121 g         1337 t
ORIGIN      
        1 aactccatca gttttcattc tccgcgtcat gctagcctga tagaccggtc tgttcaagcc
       61 gaggactgtg ctctgtcctc gagatcatgc caagatcccc ttgaccggat gatgctcttc
      121 aacaccggtc aagcgcacat ccaacccctg gaatttgagg ctcaagcggc ggtggtcgat
      181 gcccaagcaa tggagaatcg tggcgttcag gtcgtgaatg tgtactgggt cggcgaccac
      241 gttgtaactg aagtcgtcgg tctcaccgtg gacgatcccc ggcttgatgc cggcgccggc
      301 catccagaga ggaaaacact tgggatggtg gtcgcgcccg taattctcac ggctgagctt
      361 gccctgacag taaactgtcc ggccaaattc acctccccag atcacgagcg tgtcgtcgag
      421 cagcccgcgc tgcttgaggt cagcgaccag cgcaaacgtt gcctggtcga cgtcctgaca
      481 ttggagcggc aggtcgcccg cgagattggc atgctggtcc caaccacggt gaaagatctg
      541 cacgaatcgc acgtcgcgct ccgccagccg ccgagcaagc aggcagcacc gcgcgtagga
      601 tcccggcttg tgcacgtcgg gtccgtacat gtcgagcacg ctcttgggct cattcgagat
      661 gtccgtgagc tcgggaaccg aggtctgcat ccggaatgcc atctcgtact gggcgatccg
      721 ggcctgggtc tcgggatcgg caaactgttc gtattcgctg tggttcaagc ggttgatggc
      781 atcgagcgca cggcggcgag tggctgcatc gattccctgg gggttggaga ggaagaggac
      841 cgggtcgccg atggaccgca gggcgactcc ctggtgcttg gtgggcaggt aaccggctcc
      901 ccagaggcgg ttgtagagcg cctgtgcggc tgccttgctc gaccacgacg cggtcatcac
      961 gacgaatgcc ggcaagttct ggttcatggt tcccagacca taggagagcc atgagcccag
     1021 gctggggcgt cctggcaact ggtggcctgt gcagatataa gtcaccgccg gatcgtggtt
     1081 gatggcctcg gtccaaactg tcttcaccag cgcgatctcg tcggcgagcc gggccatcca
     1141 gggcaaaagc tcactcatcc atatgcccga ttggccatgt tgcgcgaact tatactttga
     1201 tggggcgacc gggaaccgcg actgcccgct ggtcatcgtg gtcagccgct gaccattgcg
     1261 gatcgattcc ggcaggtcct tgtcgtacat cgcgcccatc ttgggcttgt aatcaaacag
     1321 gtccatctgc gacggaccac cattcatgtg caggtagatg gctcgcttgg ccttgggcgg
     1381 gaaatggggc aaccccggca agccgccggt tagtgacgcg gcagaggtcg agccgccggt
     1441 gggcgtcgtg tcgcaccgcg cggcttccgc caaaagcgtc gacagcgccg ccgtgcccaa
     1501 gctcaatccc gcacggccga agaagtgccg gcgcgtcagc ccttcgatgc gttcgcgaat
     1561 cgggtccatc gttggcactc cagaatccag atcatccctt ggtgatcgct tcgtcgaggt
     1621 tcaggatcac gttgccgacc atcgtccacg cggccagctc cgacgcgcta aaatgtgcgt
     1681 caggcttggt tgcgccggcc gagatcaact gtttggcggc ttcaggctct ttggcgtaat
     1741 gggctgtcaa gtccttgaga gccgagctga gctcggcaaa gtctttgggt tccggagtgc
     1801 gggcggtcac gagccggaac atgtatgtca ggcggtcgtc ggccgtggat cccccttcgt
     1861 gcaacgtgcg ctcggccagg gcccgcgcag cctcgataaa ttgcggctcg ttcatcaaga
     1921 ggagtgcctg cagaggcgtg ttagtccgct ccctgcgcac ctgacaagac tcgcgtgacg
     1981 gagcgtcaaa agtcgtcatc tggggtgggg gggcagtgcg tttccagaag gtataaaagc
     2041 tgcggcgatg gatcttctcc acgccggtgt cggccgcgaa atgcgcggtg ttgctgtcgg
     2101 tataggcgac tgcctcccag agacctgacg gctgaggggg cttgacgctt ggccctccca
     2161 ccgtctcgac caagagcccg ccggcaaaaa gcgcctggtc acgcaccatc tcggcgtcga
     2221 gccggaaccg cgggccccgt gccagcaacc gattggacgg gtctttcgcc agagtttccg
     2281 gcgtcacgcg cgatgattgg cgatatgtgg ccgacatcac cagtcgcttc atgaatcgct
     2341 tgacgtccca gccatcttcc cgaaactgga cggccaacca atccagcagc tcgggatggc
     2401 tcggcggctc cccctgggca ccgaaatcct cggccgtttt gacaatgccc gtgccgaaga
     2461 cctggagcca gagacggttg acggccacgc gtgccgtaag cgggtgttcg ggggcgatca
     2521 gccactgggc aaaacccagc cggttcacgg gcgcacccgg cggcagcggc ggcaagaatg
     2581 cgggcacggc cctgcccact ttctcacctc tttggtcgta ctcgcccctc ttgagcaaga
     2641 acgcgggctt gggatcgccg gccttctcgc gaaagaccag ggtcgtcggg atctgatcgt
     2701 tgagctcttt tcgcttgcgc tcgagctcct ccaaattcga tcggaatggc tcgaatattc
     2761 cactggtctt ggagtaagca tgcccgacga aataagttcg cagctcgttg gtctgggtct
     2821 cggtccgctt ggatcgatcg agcgcgacga tcgccttcac attcgcgggc aaaccggttg
     2881 aacccgaggc gcgctggact cggacccagg cggcgaggga atcaaaagtc tggccctcct
     2941 gcggcgtctc ggtctcgatg ccggccttgt cccaatagac cgtaccaccg tgctgcgtga
     3001 acgcccagcc gtcgatgagc gtgccggccg cgagtttgag cttggcaaca ggcacttcca
     3061 cccgcaccca ctttttcgac gcgggtagat cgccgatcct gagccgagcg ggtgtgccgt
     3121 cggcgcccca gtcaatcagg ttctcgcccc agtacgcgcg gtgcgaccaa cccccctttg
     3181 tatgccactg gagcatcagc tcctgaggcg ggcttgatga gtcgatgtaa acatcagcaa
     3241 acaaggtgtc gcctttgccg atcttgagct tccgcgcggc gttatcgaaa aagcgctgct
     3301 tgagcccctt ggccgtgatg cgcagcgccc gtcgaccgcc aaagtcgggg gcatcggtgt
     3361 tggcgacaaa ttcccaggga ccgtctccct ggggactggc cccattgggg agggcatcgt
     3421 cgatccagac gtagtcggcc cgccctgggg cctcgctctc ggcagattct gccttggcgt
     3481 catacgcggt cgccgcgatg gccgcctcgg ccgcaatcga cttctggacc tcggcaatct
     3541 gggaatcggt cgacttgagc gcctcgctct gcttcgggtt cggcacttga gcaaccggct
     3601 cccatttgga ggaattgcca tccatcgccg gaccgtcgat gttgttgaag aacgcgaaaa
     3661 gctggtagta gtctttcgcc cgtatcgggt cgtacttatg gtcgtgacag cgcgcacagc
     3721 cagtcgtcag tcccagaaac acggtcccgt tggtatcgac ctgatcgacg atgttacgaa
     3781 cgtagacctc ctcttcgatc gagcctcctt catttgtcga cacgtgacag cggttgtaac
     3841 cggtggcgat gatctgatcg gcggtggggt taggcagcag gtcgccggcg agttgctcga
     3901 cgatgaaccg gtcgaaaggc ttgttggcat tgaaggcgcg aatgacccag tcgcgatagg
     3961 gccaggcctc acggtagtta tccaggtgca gaccgtgggt gtcgccgtaa cgcgctgcat
     4021 ccagccagac ccgcgccatt tgctcgccat agcgtggcga gtcgagcagg cggtcgacaa
     4081 gcttttcgta agcctcgcgg gatgggtcgg ccaggaaggc gtcgacctcc tgaatcgtcg
     4141 ggggcaggcc ggtcaggtcg agcgtcaccc ggcggatcaa attcgttttg gcggccattg
     4201 gcgatgccga gagcccttcg gcctcgagcc tggccaggat gaagcgatca atctcattcg
     4261 agggccagcc cgtgttcttg acggcgggaa gtgcgggctt tttcggtgga tcgaaggccc
     4321 agtgcgtcga ccagggtgct ccctgctcga tccagcggcg caacaccgcg gcctggtcgg
     4381 ccgacaattg cttgccgctc ttcttggggg gcatgcgcag gtcggacacg tcgctctcga
     4441 ttcgcagaat cagttcactg tcgtcgggct taccgggaac gatcgccagt gcgtcactct
     4501 ccagcttggc aaacgcccct tccttggtgt cgagccgtag tttggctttc cgagctttat
     4561 cgtctggacc gtggcaagaa aagcagttgt ccgagaggat tggccggacg tcgcgggcga
     4621 aattgacggg gcgtggcttg acgtcacggg tcgagggaac cggatcgcct tcgccgtgag
     4681 ccgatcccag cgtaccaagg gtcactaaca acaatgcgtt tcgcagacaa gacgccatgc
     4741 gttttccccc ttgcgtcggc cagattccac ggtggaccag aggaatagcc taaatgtcag
     4801 gccgtcgacc ccaaattcgg gtcaataacc ggccttcgaa cgtcttgaga gtcaaactca
     4861 gggaatgccg ggacgagcca tagtccagtt cgcacggtcc gactcgcctg gattcatgca
     4921 ttttacgttg ttgagagcgt tcgtgggatc aggctttagg accccgatcc gcggtgagtg
     4981 gacgccagtc tgacctgtac gccttggatg catgccagat accggtggcc atctgtaata
     5041 tacgatgctc gcgagctgtt tgaaagggct gagttgtaat tccctaacca ccgggcattt
     5101 tgcgactcgc aaaaaaatgg cgtgtcggtc ttcgatcaag tcttttagat aatggggcaa
     5161 ctgttcgagg cgatcgggag aatcggccat cacgacgttg ggcgctacac tgcggcagat
     5221 caaacgcctg tgggcggatg ggactgccat gggattctct gatgcgcaat tgctcgagcg
     5281 cttcgtgtcc gatcatgacg acgcggcgtt cgagcctttg atggcgcgtc atgggccaat
     5341 ggtcctgagc gtctgccggg gcatcttgaa agatcccaac gacgccgagg acgcgtttca
     5401 ggcaacgttc ctcatcctcg tcaaaaaatc ggctgctttc cgcgggcacg tctctctggg
     5461 gccctggctt tatcgggtgg cgcaccgagt ggcgatcagg gccaacgtcg cagccaccaa
     5521 gcggcgggcg tgggagaggg aggcaggaca gatgcatggg acatcctcaa taatgggact
     5581 cgcacagccg cccgaccaat tgcaggcgct tcacgaagag atcgcacggc tgccagagaa
     5641 gttccgtcgg gcagtcgttc tctgcgacct ggagctcgtc cctcaaactc gggccgccgc
     5701 ggagttgcgt ttgagcgagc gcactttgca gcgccgactt cgccagggac gcgagcggct
     5761 caaggctcgg ctcattcgcc ggggcctggc atcggacgac gcgatgctgg gtgccgtgtt
     5821 cctccgagag gcccattccg ccgtacccgc atcttggagc gaggcgacta ttcgggcagt
     5881 gctggccggc gtcaatcagg gcatatccac cggggtggtc tcggcggcgg cggagaaatt
     5941 gactgatgag gtgctccgaa tcatgttggt ccaaaagctc acattgggat cggccactct
     6001 cctggctgcc ggattgatcg cgtggggagc gtcggccgcc ctggtctcgc tcgccgagga
     6061 accttcgaag aaaacaatcg cgaactcgca ttcgcccgtt caacccaaat ccgaaactcc
     6121 cgttcgccaa cccgaaccgc aattgcttga ttcgaccgac aaaatcagta ttggcggtcg
     6181 ggtgctcgga accgacggac agccggttgc cgatgcgaag ctctatataa cagtgatgcg
     6241 ggggtactac cgggagccat ttcccgccaa agaaaatgcg ataaccgggc ctgacggccg
     6301 tttctcgttc acggtatcca aatcgacaat tggcgatgag aaaattgtcg cggccgctat
     6361 agccgcgaat cacggtcttg gttggctgga agttccggca gccggcaaca gacacgacgt
     6421 gacccttcgg ttggtgaacg acgacatgcc gatcaccggc caagtcgtcg atcttgaggg
     6481 gaagccggtt gcagatgcaa ccctgcgcgt gctggaaatc agcgcggcac ccggcgaaga
     6541 tctcggccgg cggctcgaag ccgcccgggg gaaaacggag gagaaatccc ggctggagac
     6601 ggaggatttc aagtggaacg ccatcaatgt gtcgcgactg gccatcaagg tcacgacgga
     6661 cgccgacgga cgtttcgtgc tcaccggcat cggtcgcaac cgcctcgtca gggcgcagct
     6721 cgacg
//
LOCUS       DATAQL010001006         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1018447, whole genome shotgun sequence.
ACCESSION   DATAQL010001006 DATAQL010000000
VERSION     DATAQL010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018447"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..>2266)
                     /locus_tag="VHS97_17405"
     CDS             complement(97..>2266)
                     /locus_tag="VHS97_17405"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX3450034.1"
                     /translation="LCHLQANLFADAERLTLRAWLASRVVNMDAERRALEKLVPIVHD
                     SWAVDRLALLAWNAGQFDLAREYRLRKAKLDTAKDHYRLMMDGPLTPDRFVELAGLAE
                     TLGHRFEARGWWSLRARYAPSDLSATEALSRLAQLRESARLGHKVTLADALCDTDPDM
                     KAGAERPRPTRPEVEALIPRFTDDAQKAGLSFTFDNGATRIRQLPETIAGGVALLDYD
                     GDGMLDVYVVQGGAFPPDPSRPNTGDRLFRNQGDGTFADATERSGIARMKRGYGHGIA
                     VGDIDNDGHPDLVVTRWRSYALYHNRGDGSFEEITNQAGLGGDRDWPTSAALADLDND
                     GDLDLYVCHYLVWDAEHPKLCRRTIVTAENERVERGQMYDYCSPRLFPALPDHLFRND
                     GGRFVDVTAEAGILDQNGRGLGVVAADVDADGLIDLFVANDTTANYLWHNLGGMRFEE
                     AGVCAGVACSADGAFQAGMGTACGDLDGDLRPDLFVTNFYGESTSFFRNMGSGIFGDQ
                     TNAIGLAAPSRFFLGFGVSLLDADNDGRLDLATANGHVNDERPDYPYKMPAQLMMGGN
                     DGRLHDVTQSAGEPWTIPRVGRGLCVGDLDNDGRMDVVLVSQGTPLVFCHNQTNSKGR
                     AVSFLLEGTRSDRDGVGAVVTVKAGGRARRAWRLGGGSFLSASDPRLHFGLGEDGIDE
                     VLVHWPSGQVDRFEHVKADHAYRLREGAAAPIPLREFHRL"
BASE COUNT          430 a          702 c          713 g          421 t
ORIGIN      
        1 gctagccctg ttttcgagga cgtctacatt cgagataggg ccaacccggg tcactccaaa
       61 tcagagtcac gatcacacaa tggtaatcga atcggatcaa agtcggtgga attctcgcaa
      121 ggggatcggt gcggccgcgc cctcgcgcaa gcgataggcg tggtcggctt tcacatgttc
      181 aaagcgatcg acctgtcccg aaggccagtg aaccaggact tcgtcgatgc cgtcctcgcc
      241 caggccgaaa tggagtcgcg ggtcggaggc cgacagaaag ctgcctcctc ccaggcgcca
      301 ggcgcgacgg gcccgcccgc cggccttgac ggtgacgacc gcaccaacgc cgtcgcggtc
      361 ggacctggtt ccctcgagca agaagctcac agcgcggcct tttgagtttg tctggttgtg
      421 gcagaagacc aatggcgttc cctgcgacac cagcacgacg tccatacgac cgtcgttgtc
      481 gaggtcgcct acgcacagcc cgcgaccgac gcgcggaatg gtccagggtt cgccagcgga
      541 ttgggtaaca tcgtgcaggc ggccgtcgtt acctcccatc atgagctgag ccggcatctt
      601 ataaggatag tcggggcgtt cgtcgttgac gtggccgttg gcggtggcca ggtcgaggcg
      661 gccgtcgttg tcggcgtcga ggaggctaac gccaaagcca agaaagaacc ggctcggggc
      721 ggccagcccg atcgcgttag tctggtcgcc gaatatccca gagcccatgt tgcggaagaa
      781 cgaagtggac tcgccataaa aattggtgac gaacaggtcc ggccgcaggt cgccgtcgag
      841 gtctccacag gccgtcccca ttcccgcctg gaaggcgccg tcggcgctac aagcgacacc
      901 tgcgcagaca cccgcctctt cgaacctcat gccgcccagg ttgtgccata ggtaattggc
      961 cgttgtgtcg ttggcgacga aaagatcgat taacccatcc gcatcgacgt cggccgcgac
     1021 gacaccaagg cctcgaccat tttgatcgag gatgcctgct tcggcggtca catcgacgaa
     1081 ccgtcccccg tcattgcgga agaggtgatc cggcaaggca gggaaaaggc gcggcgagca
     1141 gtagtcgtac atctggccgc gttccactct ctcattttcg gcagttacta tcgtgcgtcg
     1201 gcagagtttc gggtgctcgg catcccagac gaggtaatga cagacgtaga ggtcgaggtc
     1261 accgtcgttg tcgaggtcgg ccaaggcggc cgaggtcggc cagtcgcgat cgcctccgag
     1321 gccggcttga ttggtgatct cttcaaagga tccgtctcct cggttgtgat aaagggcgta
     1381 tgatcgccag cgagtgacga cgaggtccgg gtggccatcg ttgtcgatgt ccccgacggc
     1441 gataccgtgt ccgtagccac gcttcattcg ggcgattccg gatcgttccg tcgcatcggc
     1501 gaacgtgccg tcaccctggt ttcggaacaa tcggtcgccc gtattcggcc gggaggggtc
     1561 gggcggaaag gcgcctcctt gcaccacgta gacatccagc atgccatcgc cgtcgtaatc
     1621 gaggagggcg actcccccag cgatcgtctc gggaagctgg cgaatccgcg tcgctccatt
     1681 gtcgaacgtg aagctcagcc cggctttttg tgcatcatcg gtgaaccgtg gtatcaaggc
     1741 ctcgacctcc ggccgagtcg gtcgcggacg ctccgcgcca gccttcatgt ccggatccgt
     1801 atcgcagagc gcatccgcga gcgtgacctt gtgcccaagt cgcgcggact ctcgcaactg
     1861 tgcgagtcgt gacaatgctt ccgtcgcgga gagatcggag ggtgcgtacc gcgcgcgaag
     1921 cgaccaccaa ccccgcgctt caaaccggtg gcccagagtc tcggccaagc ccgccagttc
     1981 aacgaaccgg tccggcgtta gtggtccatc catcatgagc cgatagtgat ccttcgccgt
     2041 gtcgagtttt gccttgcgta gacgatattc ccgtgctaga tcaaactgcc ccgcgttcca
     2101 ggccaggaga gcgagccgat ccaccgccca ggagtcatgg acgatcggca cgagcttttc
     2161 cagcgcgcgg cgctcggcgt ccatgttgac tacgcgcgac gccagccagg cgcggagggt
     2221 gagacgctca gcatcggcga agaggttggc ttgtaaatga cagaga
//
LOCUS       DATAQL010001007         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3309314, whole genome shotgun sequence.
ACCESSION   DATAQL010001007 DATAQL010000000
VERSION     DATAQL010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3309314"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VHS97_17410"
     CDS             <1..877
                     /locus_tag="VHS97_17410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX3450035.1"
                     /translation="QFQLSLRATAKQSRAAERSMGRDCFVAQGAPRNDTIEAWLILQV
                     HPYHWRPSGSFLAHLLKATTQQHHRALAPAIARLVPSTAVVFDVGAHAGQYTKLFARA
                     ANQGRVYAFEPGSYARSILRTVVWLHGLSNVAVVPIALGSEARLDTLTIPLKNSGSFG
                     FGLSHLGKPSERWRAVAQEIVALTTVDGVVKALELDRLDFIKADIEGWELRLLHGAES
                     TLGRFRPRLLLELSSTALARAGDRLVDAFAFLAARGYRAFRLEPGASLAPVAAPVDGD
                     FWFLPADDPVGTAGF"
     gene            complement(920..>1914)
                     /locus_tag="VHS97_17415"
     CDS             complement(920..>1914)
                     /locus_tag="VHS97_17415"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX3450036.1"
                     /translation="FKVGKVDPYFLVQRGFLRQTIDLGGETEKLDPDLNQLGGTQTAD
                     RLVFTIGKYSIIDIFDTNKYAHDPRNDFFNWSIIDQGAFDYAANSWGYTYGAALEWYQ
                     DWWTARAGVFDLSQEPNSVALSHGFAQGQFVAELEERHELWEQPGKLKFLYWLTRGRL
                     GTYLDAISLGAATGQTPSTSDVRRFRTKDGFGLNLEQRLAEDFGVFARASISQGTVEE
                     VDFTEINQSVSGGLSLTGLRWGRPDDTVGLAGAINRISHQGKLYLAAGGLGGIIGDGQ
                     LPNAGPEQILEAYYNIAIVKFAQFTADYQFINHPAYNRDRGPVSIFGVRLHLQF"
BASE COUNT          343 a          571 c          609 g          391 t
ORIGIN      
        1 ccaattccaa ctgtcattgc gagcgacagc gaagcaatct cgggccgctg agcgctcgat
       61 gggtcgagat tgcttcgtcg cgcaaggcgc gcctcgcaat gacactatcg aggcttggct
      121 tatcttgcag gttcatccct atcattggcg tccgtcgggg agttttttgg ctcacttgct
      181 gaaggcgacg acccagcagc atcaccgcgc gctcgctccc gcgatcgcga ggctggtgcc
      241 gtcgaccgcc gtcgtctttg atgtcggggc gcacgccggg caatacacca agctcttcgc
      301 acgcgccgcg aatcaggggc gggtctatgc attcgagccg gggagctatg cccgctcgat
      361 cctgcggacg gtcgtctggc tgcacggatt gtcgaatgtg gccgtggtgc cgatcgcgct
      421 cggctccgag gctcggctcg acacgctgac catcccgctc aagaacagcg gcagctttgg
      481 gtttggactt tcccatttgg gaaagccgtc agagcgctgg cgcgcggttg ctcaggagat
      541 tgtggcgttg acgacggtcg atggtgtggt gaaagcgctc gagctcgatc gcctcgattt
      601 tatcaaggca gacatcgagg gatgggagct gcgactgctg cacggcgccg agagcacgct
      661 cggacgtttc cgaccacgcc tgttgctgga gctcagtagt acggccctcg cgcgtgccgg
      721 ggaccggctc gtcgatgcct tcgcgtttct ggcggcgcgc ggttaccggg cctttcgctt
      781 ggagccgggg gcaagtctcg cgccagtcgc cgcgcccgtc gacggcgatt tctggttctt
      841 accggcggac gatccggtgg gcactgccgg cttttagcca cagtgcccaa gagggcctgt
      901 cggggtaacc gcacttcaat cagaactgca ggtgcagtcg tacgccgaaa atcgacacgg
      961 gaccgcggtc ccgattgtag gccgggtggt tgataaattg gtaatcggcc gtgaactggg
     1021 cgaacttgac gatggcgatg ttgtaataag cctccagtat ctgctctggg ccggcattcg
     1081 gcaattggcc gtcgccgata atcccgccca gaccgccggc ggcgagataa agcttgccct
     1141 gatgtgaaat ccgattgatt gcgccggcca atccgacagt gtcgtccggc cgcccccagc
     1201 gcaagccggt caatgagagg ccccccgata ccgactggtt tatctcggtg aaatcgacct
     1261 cctcgacggt gccttgcgag atgctggcgc gggcaaagac gccaaagtcc tcggcgagcc
     1321 gttgttcgag attgagcccg aacccatctt tggtgcgaaa acggcggacg tcggaagtcg
     1381 acggcgtctg accagtggcc gcccccagcg agatcgcgtc aagataagtc ccgagccggc
     1441 cgcgggtcag ccaatagagg aatttgagct tgccgggctg ttcccaaagc tcatgtctct
     1501 cttccagttc ggccacgaac tgcccttgtg cgaagccgtg ggacagcgcg accgagttcg
     1561 gctcttgcga caggtcgaac accccggccc tcgcggtcca ccaatcctga taccattcga
     1621 gcgcggcgcc gtaggtgtag ccccatgaat tcgccgcgta gtcgaatgca ccctgatcga
     1681 tgatcgacca attgaagaag tcgttgcgcg ggtcgtgcgc gtatttgttg gtgtcgaaaa
     1741 tgtcgatgat ggagtatttg ccgattgtga acaccaggcg gtcggcggtc tgagtgccgc
     1801 cgagttggtt caggtcagga tcgagctttt cagtctcgcc gcccaaatcg atcgtctggc
     1861 ggaggaaccc tcgctggacc agaaaataag gatccacctt gcctaccttg aagg
//
LOCUS       DATAQL010001008         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1322978, whole genome shotgun sequence.
ACCESSION   DATAQL010001008 DATAQL010000000
VERSION     DATAQL010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322978"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2485
                     /locus_tag="VHS97_17420"
     CDS             <1..2485
                     /locus_tag="VHS97_17420"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3450037.1"
                     /translation="NARKSLAAVTTVSAIQGDLAKIRNHDLGSTIDYAPNAAEETERA
                     PDATMPETADATRIGEARFRLLNFHARGGLGEVWVAQDVELNREVALKRIQEKHADSS
                     ESRTRFLHEAEVTGRLEHRGIVPVYGLGHFENGRPYYAMRFISGTSLKDAIAKFHGAK
                     NTDPAAVDRSLDLRRLLGTFVDVCNTVAYAHSRGVLHRDLKPDNIILDDYGETLVVDW
                     GLAKTGAIDVDSALNVGTSPPVSTSSTPYTLAGTTLGTPGFMSPEQASGRLSDLRPAS
                     DVYSLGATLYCLLTGKTPFNEPDPILILPKVCAGDFPRPREVKSKVPVALEAICLKAM
                     AVSPDGRYSSPRAFGDEIERWLADQPVLAYPEPFSARALRWVRRRKQWVAAAAVLLIL
                     TVLGLAIYNWLITAEKGRTTEQLAMTRAALRELLKVSGENLTVIPNTEKLREHLAQLV
                     LDRYHQLGDRFPSDPSVRFETAQVLHVIGAIGGLTGQFAKSQASYEKAIRELTNLCEN
                     DPGRVDYRRWLVEVFIDRGELNHMNGRTIDAENDFQAAISHADRLRSEPLSPNFRRTM
                     GSALINLSEILVLKCQFAEAHTAADRAVNLLRPLAGPGAGPDATTRDRWLLAMSLTDR
                     GVASRESGNRDSAAHDFDEAAHVAGSVAPDDEVYDDAQFQLATIANQRGDLLRMDPAK
                     VHESENNYVQAAQILTGLIGNHKLIPHYREEMAVTLCGRAAVRLAMNRIPDAQQDCEA
                     ALVHLDWLTAEQTRKGGPENPQYLSLRGQVLSVQSRIHFLQVRLPDGRSARAQAVEKL
                     SRAIELDPARAADKVLLEQINAGPAQLEQ"
     gene            complement(2490..>2776)
                     /locus_tag="VHS97_17425"
     CDS             complement(2490..>2776)
                     /locus_tag="VHS97_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450038.1"
                     /translation="VRARNHPYAVRRTEMVRAPFGQTPRSISMNPRNGINRKYRPCFE
                     ALERKQLLSAGLLTHGAQALVQSTAPAPSNVQHQGVVPDGTGKGIVIITS"
BASE COUNT          559 a          855 c          855 g          507 t
ORIGIN      
        1 caacgcgcgt aagagtctgg ccgcagtgac gaccgtcagt gcgatccagg gcgatctcgc
       61 gaagatccgc aatcacgatc tcggttccac aattgactac gcgccgaacg ccgcggaaga
      121 aaccgaaagg gctccagacg ccacaatgcc cgagaccgcc gatgctacac gcatcgggga
      181 agcgcgattc cgactcctta acttccacgc ccgaggcggc ctcggggagg tttgggtcgc
      241 ccaggatgtc gaactcaatc gggaagtggc gctcaagcgg atccaggaaa agcacgccga
      301 cagttccgag agccggaccc gcttcctcca cgaagccgaa gtgaccgggc ggctggagca
      361 ccggggcatc gttcctgttt atggtctcgg tcactttgag aatggacgtc cctattacgc
      421 catgcgtttt atcagcggga ccagcctcaa ggacgcgatc gccaagtttc atggcgccaa
      481 gaatacagac cctgcggcag tcgaccgctc gctggatctc cgccggctcc tcggcacgtt
      541 tgtcgatgtg tgtaacacgg tggcctacgc gcatagtcgc ggagtcctcc accgcgacct
      601 gaaaccggac aacatcattc tagacgacta tggcgagacg ctggtggtgg actggggatt
      661 ggccaagacg ggagccatcg atgtggattc cgcattgaat gtgggcacga gcccgccggt
      721 ctcgaccagt agcacgcctt atacactggc aggtacgacg ttgggcacgc ccggcttcat
      781 gagtccggag caggcatcgg gccggctttc tgacctacgc cctgcaagcg atgtgtacag
      841 cctcggggcg actctttact gcctgctgac cgggaagacc ccgttcaatg aacctgaccc
      901 gatactgatc ttgcccaagg tctgcgcggg ggactttccc cggccccgcg aggtcaagtc
      961 gaaagtgccc gtcgcgctcg aggcgatctg cctgaaagcc atggcagtct caccggacgg
     1021 tcgatattcc agtccccggg cttttggcga tgagatcgag cgctggctgg ccgatcaacc
     1081 tgttctggca taccccgagc cgttctcggc cagggccctg cggtgggttc gccgccgcaa
     1141 gcaatgggtt gcggcggcgg cggtcctcct catcctgacc gtgctcggac tggcgatcta
     1201 caattggcta atcacggcgg agaaagggag gacgaccgaa cagctcgcca tgacccgcgc
     1261 cgcactccga gagctcctca aggtctcggg ggaaaacctg actgtgattc ccaatacgga
     1321 gaaactgcgt gagcacctcg cgcagctcgt tctcgatcgg tatcaccaac tgggcgacag
     1381 attcccgagc gacccgagcg tccgcttcga aaccgcccag gtgctgcacg tgatcggggc
     1441 aatcgggggc ctcaccggac aatttgcaaa atcacaggcg tcttacgaga aggcaatccg
     1501 ggaattgacg aatctgtgcg agaacgaccc cggccgggtt gactaccggc ggtggctggt
     1561 ggaggtcttc atcgatcggg gagagctcaa ccatatgaac ggccggacga tcgacgcgga
     1621 gaatgatttt caggcggcaa ttagccacgc cgacaggctt cggtccgagc cgctctcccc
     1681 aaatttccga cgcacgatgg ggtcagcgct catcaatctt tccgagatcc tggtcctcaa
     1741 atgtcagttc gcggaggctc acacggccgc tgatcgcgcc gtaaacctgc taaggcctct
     1801 tgccggtccg ggcgccgggc ccgatgcgac gaccagagat cgatggttgc tcgccatgtc
     1861 gctcacggac cggggcgtcg cgtccaggga atcggggaat cgcgacagcg ccgcccacga
     1921 tttcgacgag gcggcgcacg tcgcggggtc ggtcgccccg gacgacgaag tctacgacga
     1981 cgctcaattc cagctggcca cgatcgcgaa tcagcgagga gacctcttga gaatggaccc
     2041 tgcaaaggtc cacgagtccg agaacaacta tgtccaagcg gctcagatcc tcacagggct
     2101 gatcggcaat cataaactaa ttccacatta tcgcgaggag atggcggtga cgctgtgcgg
     2161 ccgggcagcc gtccgactcg ccatgaaccg catcccggac gctcaacaag attgcgaggc
     2221 cgcgctggtt catcttgact ggttgaccgc agaacagaca cgtaagggtg gcccggaaaa
     2281 cccccaatac ttaagccttc gtggtcaggt cttgtctgtg cagagccgga ttcatttcct
     2341 gcaggtccgg ttgccagatg gtcgcagcgc ccgcgctcag gcggtggaaa aactgagccg
     2401 ggccatcgag cttgaccctg cccgtgcggc ggacaaggtc ttgctcgaac agatcaatgc
     2461 cggcccggct cagcttgaac agtaagttat cacgatgtga tgataacaat tcccttcccg
     2521 gtgccatccg ggacgacgcc ctggtgctgt acgttcgatg gcgcgggcgc tgtggactgc
     2581 acgagtgcct gtgccccgtg ggtcagcagg ccggccgaca agagctgctt gcgctcgagg
     2641 gcctcgaagc atggacgata ctttcgattg atgccattgc gcgggttcat ggaaatgctt
     2701 ctgggcgttt gcccgaatgg ggcgcgcacc atctccgtcc tccgaacggc gtacggatgg
     2761 ttacgagccc ggactt
//
LOCUS       DATAQL010001009         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1782646, whole genome shotgun sequence.
ACCESSION   DATAQL010001009 DATAQL010000000
VERSION     DATAQL010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782646"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..427)
                     /locus_tag="VHS97_17430"
     CDS             complement(26..427)
                     /locus_tag="VHS97_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450039.1"
                     /translation="MTEVAVIAKPDLQGKRLSFALAEDRVAHYPEFRDFFVRTFDLDR
                     KGLAEPGFVRAPSGITYALVFIGRSGEPFPSGVEIHAIVDALEPLEGTILDRDLWSIL
                     RWMVGGVGAPWTVEDFDRTGRLYRVPAAPPA"
     gene            complement(431..1264)
                     /locus_tag="VHS97_17435"
     CDS             complement(431..1264)
                     /locus_tag="VHS97_17435"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3450040.1"
                     /translation="MHYIDEGEGDPVVLLHGNPTWGFLYRNMIGPLVNSGRRVIVPDM
                     IGFGLSEKPTREAAHSLDGHSANLTALMRQLDLTRITLVCHDWGGPTGLSFAMSNPAR
                     VRALTIMSTWAWPLPPAEFHTRIFPWRMMHAPLVGPYLLGNHRALAGRGVYLSVVNRE
                     QFAHAAQGAYEAVLPDPATRLLTWVWPRWIPLDENARAYERFRWLEQELSRSKFPTLI
                     IWGREDEVFDATTFSNRFKQLLPHAEGPHLVTGRHFLQEDSGPEIAGLIRSFLKRLDG
                     G"
BASE COUNT          354 a          465 c          491 g          274 t
ORIGIN      
        1 tagccgccgg ttgacagaac ggagatcagg ccggcggggc cgccggcaca cgatacaaac
       61 gtcccgtccg gtcaaaatcc tcgacagtcc agggagcgcc gacgccgccg accatccatc
      121 gcagaatcga ccagagatcc cggtccaaga tcgtcccctc gagaggctcg agcgcatcca
      181 cgatggcatg gatctcaacg cccgatggga agggttcacc gcttcggccg atgaaaacaa
      241 gcgcgtaggt aatgccggac ggagcgcgga caaaacccgg ttctgcgagc ccctttcggt
      301 ccagatcaaa agttcggacg aagaagtcgc ggaactcggg ataatgcgcc acgcgatctt
      361 cggccagagc gaacgagagc cgtttgccct ggagatccgg cttcgcaatg actgccacct
      421 cggtcatcgc tcatcctccg tcgaggcgtt tcagaaagga gcggatcagc cccgcgatct
      481 cgggaccgga atcctcctgc aggaaatggc ggccggtgac aagatgcggt ccttcggcat
      541 ggggaagcaa ttgcttgaag cggttcgaaa aggtcgtggc gtcgaagacc tcgtcctcgc
      601 gcccccagat gatcaaagtg ggaaacttcg accgagatag ttcctgctca agccatctga
      661 aacgctcata ggcccgggcg ttctcatcaa gcgggatcca gcgtggccaa acccaggtga
      721 ggagacgcgt ggcggggtct ggcagcacgg cctcgtaggc accctgagct gcatgggcaa
      781 actgctcacg gttgactacc gagaggtaga cgccgcgacc ggcaagcgca cgatgatttc
      841 ccagaagata gggtccgacc agcggggcat gcatcatacg ccacggaaaa atgcgcgtgt
      901 ggaattcggc cggaggcaag ggccatgccc aggtgctcat gatcgtgagt gcacgaacgc
      961 gagcaggatt gctcatagca aacgatagcc cggtcggccc gccccaatcg tggcatacga
     1021 gcgtgatccg ggtcaggtcg agctgacgca tgagcgccgt aagattggcc gagtgtccat
     1081 cgagagaatg agcagcttcg cgggtcggct tctcggaaag gccaaacccg atcatgtccg
     1141 gaacgatcac gcggcgcccc gaattgacga ggggaccgat catgttgcgg tacaggaatc
     1201 cccaagtcgg gttaccgtgg agcaggacga cggggtcacc ttccccctcg tcaatatagt
     1261 gcatgcgtca gccattgacg tttgcgtaac gcggcacata cggccactgg gcccgtaacg
     1321 tcgcgggcag cgtgctctcc gactggacgg tcacaaatcg ctccgctcac tttcggccag
     1381 tgttctctta catgtgtcga ggccggactg gttgattata gatcgtccga tcaaaaatga
     1441 gagctcgaat cgccgtctcc atccggactt cggccctgtg ggatggagcc tgcgcgagcc
     1501 tggcaattgt caggcgacca tatcgagtgc tatgcaatta tgctacggcc gtcgaggccg
     1561 atcgaaagga gatatgcgac aatg
//
LOCUS       DATAQL010001010        13097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1171075, whole genome shotgun sequence.
ACCESSION   DATAQL010001010 DATAQL010000000
VERSION     DATAQL010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171075"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1102)
                     /locus_tag="VHS97_17440"
     CDS             complement(89..1102)
                     /locus_tag="VHS97_17440"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3450041.1"
                     /translation="MNQPMACDVKTARLLLAYGANHDLTVAAALGDFDRVTAILNEDP
                     SQIREARPNGRRPLTTAVEFGHESIARFLLDRGANPTWPELNADKGGALHAAARAGNR
                     AMVELLLSRGADPNGHTDSGGNAVCAAKTSEIRALLEAHGGSLDPYDLVWKDEDDEVM
                     RRVTADPKSAELGCGGVFTAVVTRGKRDLLKRLLDAGIRVPPLVTGCQSYLMEQPDMF
                     RTLLESGMNPDTCNWQMQTMLHMCCRGDSSGRPDKVSLECAAMLLDAGANILARDDDY
                     CSTPLGWAARHNRPDMVEFLLARGAKTNLPDDKPWATPLAWAKRRGHTQVAEMLVRAG
                     AKG"
     gene            1265..1696
                     /locus_tag="VHS97_17445"
     CDS             1265..1696
                     /locus_tag="VHS97_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450042.1"
                     /translation="MNVCATFKEQLDNAARPSDHSTMERRASRMIPTIQEVRVRIQKF
                     TNPSKVACLRSQMNRLILTCLSRRGPTLAIAGPFERCRDSIMAAFPCHSDQAVAIVPV
                     PFRVRSCIEQQLNDFGMCFAGGEVNRRRIELAATAERRIPV"
     gene            complement(1790..2488)
                     /locus_tag="VHS97_17450"
     CDS             complement(1790..2488)
                     /locus_tag="VHS97_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEX3450043.1"
                     /translation="MGMPTASYRGRVRFTGALNDYSGSLPPLLIAVALVGGYCEAGHA
                     KAQDEFPVPDSPAAPPTKIVIPPEGEPGEPLVISGTIYQPDGRTPAAGVTLYVFHTDA
                     TGVYSPDQAEGKLPKPRLQGWVRTKADGRYEFRTIRPTAYPGGDTPAHIHAGVYGRGV
                     RYWINDYWFADDPLITSKQRKRVDGPEGSGKVIVTLKRDDKGIWRGTRDITLKKVETT
                     PWSNIRAVAKSTTS"
     gene            complement(3054..3470)
                     /locus_tag="VHS97_17455"
     CDS             complement(3054..3470)
                     /locus_tag="VHS97_17455"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX3450044.1"
                     /translation="MAENKTKATNASVDAYLAAIADPGRRSDCDALARLMAKVAGQPP
                     KMWGTSIVGFGSYHYKYASGREGDTCLLGFASRKGDITIYGLGTFPGREELLSNLGKH
                     KMGKGCVYISKMSDIDVKVFEQLLKKSVAESKKQHG"
     gene            4009..4365
                     /locus_tag="VHS97_17460"
     CDS             4009..4365
                     /locus_tag="VHS97_17460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3450045.1"
                     /translation="MAVAADGISCTAWKRPGERPPWADTPGVTGSSTSVAMAREAAGV
                     VICDINPKTLPRARAEVEAAGVRSLAMECDVPSGGSVAELFRETVSTFGTLDVLVNDA
                     APVQSGACDEEMRPEF"
     gene            4431..4757
                     /locus_tag="VHS97_17465"
     CDS             4431..4757
                     /locus_tag="VHS97_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450046.1"
                     /translation="MAPVLRGEWPRRVLLQARGPGDHGIEAVGADHHRLDRRHRRVRR
                     LPPGVPRHQGAVPAFTRSVAVEVAGASVFVNAIPGGGILTPPRAVYLASGEHHLVISP
                     NGGMII"
     gene            4875..5375
                     /locus_tag="VHS97_17470"
     CDS             4875..5375
                     /locus_tag="VHS97_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450047.1"
                     /translation="MSNDQDTQRPESDAELEREIREGRKFTLAEAIGRLAGPGAMKGE
                     SPVARLQQAECEIEYWLRSHLADARGTLRVVLHRHVKGSELLLNGFDQTLLVLAGYCQ
                     RVLDSDYLLEELVRDADIEWGRLMGERPYFEKEGSPRHPDDPYTVESVRNALSGLLEQ
                     LAVGEG"
     gene            6041..8683
                     /locus_tag="VHS97_17475"
     CDS             6041..8683
                     /locus_tag="VHS97_17475"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX3450048.1"
                     /translation="MNSRMNRIGLALSGGGFRASLYHLGLVRLLRDGGILPRVTHITS
                     VSGGSIFAGHLVLNWDRYNGSPSEFDAAASEFLSFVRLDVRNRITRRFPLTLPLRWPR
                     RLLGRSNRKLTRTGLLEYYYDKYLYGDTSLFELPEKPALHLLATNLSEGCLCSFHRDG
                     LLMVRRQPGNSFRIDRIHIGLATVPMAVTASSAFPGFFPPLVLTGADVGANPGEFGRQ
                     AYTDGGVFDNLGVRMFGLLERPLLAESPLSREDFIDFPAFVEALREASKSSQDTPLGR
                     FAQILVAASRRPDPLMLTVAGTSSGAGFQPVAGPGPVGSPPHSTPPPGASKGDGEAVL
                     LSILWDLMRHYPFHREPLFAGLKPLDPEAEALLHDSRPGGRDPADSDQLWLNRHLIEA
                     AFRQTTGRACFRRLHSGLDGVLVSDVGMPISVQGNRRAGGLIRTALRATDILMHRVWQ
                     LEIETFQDTPGFVFAPITEVVEHAEDPTALHPEIQRQAAKIRTDLDRFSLLEISSLVR
                     HGYCVGRKACRARPDLFGSELPNDAPWDPIPGPRDAVPAAPVATRPSRRLRSPLLGRG
                     VGGKVEPAAAAVEARTLQASALRRIWSTLLDPLDWMTYVYVPLIVPILILLPYFCVKY
                     YQRSHRMNQLVESLSHGSPDSQQMSLLLEGPMKPWIGEAAEEVRNLDEPDYKGFEILQ
                     DSRMLDLRKWTPTASGEGASSSSVYGYRRLKLLKQPENTGNNLFRFRLLPTSPLAQVR
                     FPLQQLRPRLRKSYVEGAVPGEKSCLWEASVDFQRVPAGDYVDLTYEHLSPGEFLQHS
                     EGSDTVSSEIRAKTAELTRWILLPEGKEYRGFRTIRYKTGKPETAEAVKIVTEYLAED
                     YTILAFKLLLLEPGYTYDVTWYYE"
     gene            complement(8674..9189)
                     /locus_tag="VHS97_17480"
     CDS             complement(8674..9189)
                     /locus_tag="VHS97_17480"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEX3450049.1"
                     /translation="MCATSRCACGSCRGSRARRPIRSTCRFISRAGYSPPSRAGNATA
                     TSIDQAVLWVLDAHLRGLLDVCGGCEKIRTTPPSPTYVGLLRAGLVLNVLAAPWLTVP
                     DIGAWSLPVILMVCFFLFGVEVIDSVVEEPFGRERDDLDLDRYCRSIRESVDASLPFT
                     PGQSGRASPYS"
     gene            complement(9823..10791)
                     /locus_tag="VHS97_17485"
     CDS             complement(9823..10791)
                     /locus_tag="VHS97_17485"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3450050.1"
                     /translation="MELRRYRHVLALADHASFRKASEALGISQPALTKSLFQIERDLG
                     QRLFDRHGQTISPTVFGAIVVDTARKMLDSQEAMTRAIGHTAALDAGELAVGVGPYTA
                     DTWMGQVSGRLLERYPRLRISIHVEPWESLPDLLHTGRVDLFVASIEQVRGRKEFRVV
                     EFPEQRGIWICRAGHPLTGQASPSRSALCQYVMISPQLTPRIQRWLEAAETPSRGFRR
                     RVNTTSVTMIKAMVRQSDAVTLIHPDEVRVELARGDFVPLDIGAPPLTFHTGLVWLAD
                     RSLSPAGMAFAREMFAEVGVDAESSLRSSAVGGKAESGSWATSGSV"
     gene            11038..11283
                     /locus_tag="VHS97_17490"
     CDS             11038..11283
                     /locus_tag="VHS97_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450051.1"
                     /translation="MTSVLSRACLAGMMMVLLALAYAGCGPSSPSDEPKTENQARDEK
                     KIQELSRTGYNFSEIRSIMKGEQPKPRPKKKTGLARR"
     gene            11521..12663
                     /locus_tag="VHS97_17495"
     CDS             11521..12663
                     /locus_tag="VHS97_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3450052.1"
                     /translation="MKTRIRRRAFTLIELLVVIAIIAVLIALLLPAVQAAREAARRIQ
                     CTNNLKQVGLAIHNYVSANNSLPSGVVFNTSVAPCSGSGFGKSCQNTTWFVLTLPFIE
                     GGNLFNTFNFAIGSEGPMALTGYPYGYAVNSTVLTTHIGFFQCPSDTEKTFDVASSTV
                     GAVLSSAPKLSVTKGNYGVNWGNTDYGQGQRGDSWFASNPSTHRPAPFGYNQGATGPL
                     RATIASFTDGTSNTLLLSEMLQGANDDIRGLLWTDFPGSHSFVTRLAPNGNVDFWAGS
                     AYLDPAGGDTMDNLPTYGTSEPGTSPANIANGDLCDNQPGFNLPCFSQGKEGGSFAAS
                     RSRHPGGVNSLLADGSVRFFKNSINLPTWVALGSMAGGEVISADSY"
     gene            12790..>13097
                     /locus_tag="VHS97_17500"
     CDS             12790..>13097
                     /locus_tag="VHS97_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450053.1"
                     /translation="MSTRSRGIGVLTVAMAIMAAGRPGIALGASEGDEFRVGAADKEI
                     TPPPGIPMWGYGPRHDKPSEGTLDPLMAKAIVIAAGDDKVAVVGTDLGRGPTEAMMAI
                     "
BASE COUNT         2385 a         4267 c         4101 g         2344 t
ORIGIN      
        1 gaggtccgat ttagccgcgt cattggcggc gctgagattc acagtcagtt tgccgacgag
       61 ggagcccccg aacgtgaccc gacgcacctc accccttcgc ccccgccctt accaacatct
      121 cggccacctg cgtatgtccg cgtcttttcg cccacgccag cggcgtggcc cacggtttgt
      181 catccggaag attcgtcttc gctccgcgag cgagaagaaa ctccaccata tccggacgat
      241 tgtgtcgtgc ggcccatccc agcggcgtag agcagtagtc gtcatctctg gccaagatgt
      301 tcgctcccgc gtcgagcagc atcgccgcac attcgagtga gaccttgtcc gggcggccgc
      361 tactgtcgcc cctgcagcac atatgcagca tggtttgcat ctgccagttg caggtgtccg
      421 gattcatccc actttccagc agcgtgcgaa acatgtcggg ctgctccatc agatacgatt
      481 ggcatccggt gacgagcggc ggcacgcgaa tacctgcgtc taacaaacgc ttcagcaaat
      541 ctcgcttgcc gcgcgtgacg actgccgtga atacaccacc gcatccgagt tctgcggact
      601 tcggatccgc tgtgacgcgg cgcatgacct catcatcttc atccttccac accaggtcat
      661 agggatcgag gctgccgccg tgggcctcga gcaacgcgcg aatctccgac gtcttcgccg
      721 cgcagaccgc attgccgccc gaatctgtat ggccgttggg gtctgccccg cgcgacaaca
      781 acagttcaac catcgcgcgg ttcccggcgc gcgcggccgc gtgaagggcc cctcccttgt
      841 cggcgttgag ttcgggccag gtcgggtttg ctcctcggtc gaggaggaat cgagcaatcg
      901 attcgtgacc gaattccaca gccgtcgtga gcggccgccg tccattcggc cgcgcttccc
      961 gaatctgcga cgggtcctca ttcaggatcg ccgtgacacg atcgaagtca ccaagcgccg
     1021 ccgcaactgt tagatcatgg ttcgcgccgt atgccagcag cagtcgggct gtcttcacat
     1081 cgcaggccat cggttgattc atgcgcccgc gcgacttgat ccagaaacgc aaacacgcga
     1141 gaagcatccg ccatctcggc gggcgcacac tcctgccgcg tccaccccag accgcgagat
     1201 cgattgcctg aatattgtgc gggctgaagc cgccgggaga ccccaagccg gcaccgtgta
     1261 tcgcgtgaat gtctgcgcca cgttcaagga gcagctcgac aacgctgcgc gcccttccga
     1321 tcacagcacg atggagcggc gtgcctcccg catgatcccc acaattcaag aggtgagggt
     1381 ccgcatccag aagttcacga acccgtcgaa ggtcgcctgt ctccgcagcc aaatgaatcg
     1441 gctgatcctg acgtgtctca gccggcgcgg tcctaccctg gcgatcgcgg gcccgttcga
     1501 gcgttgccgc gacagcatca tggccgcgtt cccttgccat tccgatcagg ctgtcgccat
     1561 agtacccgtt ccattccgag tccgctcctg catcgagcaa caactgaacg atttcggcat
     1621 gtgcttcgcg ggcggcgaag tgaatcggcg gcgtatagaa ctagccgcga cggctgagcg
     1681 taggatcccg gtctaaaagc cgacgcagca tgggagcatc gccggcagcc gcggcaacga
     1741 tgatttccca gttgtcgtct tcgatgtcag gtggcttgat catgctgccc taagatgttg
     1801 tagacttcgc aacggctcgg atgttgctcc agggtgtggt ctcaaccttt ttgagagtga
     1861 tgtcacgggt cccgcgccag atccccttgt catcgcgttt cagggtgacg atcaccttgc
     1921 cgctgccttc gggcccatcg accctcttcc gctgctttga ggtgatgagc gggtcgtcgg
     1981 cgaaccaata gtcgttgatc caatagcgga cgccccggcc atagacgccg gcgtggatgt
     2041 gagccggcgt gtcaccgccc gggtaggccg ttggcctgat cgtgcggaac tcgtaccgcc
     2101 cgtcggcctt ggtccgcacc caaccctgga ggcggggctt cggcagcttg ccttccgctt
     2161 ggtcggggga gtagaccccc gtggcgtcgg tgtggaagac gtagagggtc acgcccgcgg
     2221 ccggtgtcct gccgtccggc tgatagatcg tcccggagat gaccagcggc tccccgggct
     2281 ccccttcggg gggaatcacg atcttagtag gcggagccgc cggactatcc gggaccggga
     2341 attcgtcctg agccttggcg tgaccggctt cacagtatcc cccaacgaga gcgacggcga
     2401 tcagcagtgg cgggagcgag ccgctgtagt cattgagcgc cccggtgaat cgcacgcggc
     2461 cccggtaaga agcagtcggc atacccatga tatgagtccc ctggattgga gagacccggg
     2521 ccgattcacg tggagatgtc cgtcgctgaa ctcgtgtagc ctggttacga aaatagtcga
     2581 tcgaaatccg gcccacaacg aaaaacctgg tggcgtccct gcccttgatt cagcgaactc
     2641 cttggaggcc aggttgccca gggcgctgtg cgggcgccgc tcattgtact cgacccgtgt
     2701 gagtgcatac gtcagcgctt cggtccgaca caccgaagcc gattgatcct aagttgtttc
     2761 ggtcgatccg accgactcgc gcaaggcgac cgtccgacgc tttggtcgat ccgaccgacg
     2821 cgcgcaaggc gagcgtccga cgcttcggtc gatccgaccg atggcgacaa aggtcagagc
     2881 gcatccagcc ccgaacgtac tctccggatg cgtcggtaag atcagactgc gatcagaccg
     2941 aagcgcgcca ccttccggtc cgtttggctg gcgtatcagg cgagccagtt ggttgacaag
     3001 tccgacagcg acccgtatcc gaggtccgct ccacggcgac cgtggcaaag gcgctatccg
     3061 tgctgcttct tggattccgc gaccgacttc ttgaggagct gctcaaagac tttgacgtca
     3121 atatcgctca tcttgctgat atacacgcat cccttgccca tcttgtgctt gcccaggttc
     3181 gagaggagtt cctcgcgccc ggggaacgta ccgaggccgt agatagtgat gtcgcctttg
     3241 cgagatgcaa atccgagcaa acaagtatcc ccttctctcc cactcgcgta cttgtagtgg
     3301 tagctgccga agccaacgat gcttgttccc cacatcttcg ggggttgacc tgcgaccttg
     3361 gccatcaacc tggccagtgc atcacaatcc gagcggcgac ctggatccgc gatggctgcc
     3421 agataagcat cgacgcttgc gttagtcgct ttggtcttgt tctcagccat cgggcctccg
     3481 gcgtgctagg cgtgtgcggc aactgtcgcg cgccccgcac attgcttcga caagtacgca
     3541 cctttgacac tcacaaacga acgttgccgc cggaaatcgg gccgttccac gaatgagttc
     3601 agcggaccgc cctccgatcg gaggttatca cgtcacagat tgcggttcaa tatgtggggt
     3661 ccgttcctgg gtgggctcag gcaccggctt gctcattttc atgcgcaccg caactgcatc
     3721 ctgcgcgtgt cggtccgacg aaagaggagc gacttcggta agatcagacc gaagcgggca
     3781 atcacatcct tggctgcgtc ggtcgatccg actgaagcgc gcaaggcgac tgttcgacgc
     3841 ttcggtcgat ccgaccgaag cgcgcaagcc ctcgactggc gatggtccga aagccctgtc
     3901 ttcgccaaag tcgtcccacc cgtttgtggc cagagaacag cggggcgacg ggaactaccc
     3961 ggccgtccta cgctagccga actcagcctt ggtcacgatg tgggatttat ggccgtcgcg
     4021 gcagacggaa tctcttgcac ggcctggaaa cgacccggcg aacgaccgcc gtgggcggac
     4081 accccggggg taaccggctc gagcaccagt gtcgcgatgg ccagggaggc ggccggcgtc
     4141 gtcatctgcg acatcaaccc caagaccctg cccagagccc gcgccgaggt cgaggcggcc
     4201 ggtgtgcgct ccctggcgat ggagtgcgac gtcccgtcgg gcggcagcgt cgccgagctg
     4261 ttccgggaga cggtatcgac cttcggtacg ctcgatgtcc tggtcaacga cgccgcgccg
     4321 gtccagtccg gggcctgcga cgaagagatg cggcccgagt tctagcggat gctgacgacc
     4381 cccgtcccca aggagtcgct cggggtgagc cgcgacttcc ccgacgagga gtggctccgg
     4441 tactacgggg tgaatggcca cggcgtgtcc tactgcaggc gcgcggccct ggggatcatg
     4501 gaatcgaggc agtcggggcc gatcatcatc gcctcgatcg ccggcatcgg cgggtacggc
     4561 gccttccgcc cggcgtgccg cgccaccaag gggcggtccc ggccttcaca cgctcggtcg
     4621 ccgtcgaggt ggccggggcc agcgtcttcg tcaacgccat ccccggcggc ggcatcctga
     4681 ccccgccgag ggcggtttac ctcgcctccg gtgagcatca cctggtgatc agcccgaacg
     4741 gcggtatgat catctgacgt ggcgggtgaa agcgggccgc cgggccgggt gacagcggag
     4801 gcggtcttcc gacggccgaa ggcgttcggt acgggagcga ctcaggcact ggcaatctcg
     4861 gaggcggaag gcacatgagc aacgaccagg acacgcagcg tcccgagtcg gacgccgaac
     4921 tcgaacggga aatccgtgag gggcgtaagt tcaccctggc ggaagccatc gggcggttgg
     4981 ccggtcccgg cgccatgaaa ggcgagtcgc cggtcgcgcg cctacaacag gccgagtgtg
     5041 agatcgagta ttggctgagg agccacctgg cggacgcaag gggcacctta cgagtcgtgc
     5101 tgcaccgtca cgtcaagggg agcgagctct tgctcaacgg ctttgatcaa acgctgctcg
     5161 tcttggccgg ctactgccaa cgcgttctag actcggacta cctcttggaa gaactcgttc
     5221 gcgatgccga catcgaatgg ggacgcctga tgggtgagcg gccctacttc gagaaagaag
     5281 gctctccgcg ccaccccgac gacccctaca cggtcgagtc cgtccggaac gctttatccg
     5341 gactcctcga gcagctggcc gtgggcgaag gataggacct atggtgttca ggagcggacc
     5401 ccgaggagca aatggaagtc cccacccatc taccccacgg aggttagaac accgcatcgt
     5461 tacggcctcc tcgctagtag tactccgtta acaaattggg gtggatagga agttcataga
     5521 agatgagatc gatcgggaga cctgtttccc tgggatatcg tgacgggcga ggccatcgga
     5581 gaaactcatc tttttgggtc tatggataca agtcctgtct atcaagtgct ttacggattt
     5641 aacggagtag tactagcggg tgattctcac tgattccctt ctacgcgagc gaagccttct
     5701 tatgcaaatc actattggca aagggcttgc ggaaaactca cgtgcccgtc ggtcgatcag
     5761 cccggagcgg gtaagagtat cttggattcc gcatcgggct tgatcctcgt cagggatcac
     5821 ggcgcccgtg ccgccgtcct ccgcgacctc cggcagttca ttcccggaag atcctcgcca
     5881 cgaccggtcg atcgaggggg ttcaactccg cttccgtgtg gaaggaccta cggccgcgtg
     5941 tcgtccgtgc cgggctttct acgttattat tttcgcggcc cgatgagcgg gaaagagggt
     6001 cgataccgac gtgatggaca acaccccgag gagctgcggc atgaactccc gcatgaatcg
     6061 gatcgggctg gccctctcgg gcggcggttt ccgggcgagc ctgtatcacc tcggcctggt
     6121 ccggttgctc cgcgacggcg gcatcctgcc ccgggtcacc cacatcacct cggtgtccgg
     6181 cggcagcatc ttcgccggcc acctggtgct gaactgggac cgatacaacg gctcgcccag
     6241 cgaattcgat gccgcggcct ccgagttcct ctccttcgtg cggctcgatg tccgcaatcg
     6301 gatcacccgt cgcttccccc tgacgctccc gctgcgctgg ccgcgccggc tgctggggcg
     6361 ctcgaatcgc aagctgacgc gcaccgggct gctggaatac tactatgaca agtatctcta
     6421 cggagacacc agcctgttcg agctgcccga gaagcccgcg ctacacctcc tcgccaccaa
     6481 cctgagcgag ggctgcctgt gctcgttcca ccgggacggc ctcctcatgg tgcgccggca
     6541 accgggcaac tcgttccgca ttgaccggat ccacatcggc ctggcgacgg tgcccatggc
     6601 ggtcacggct tcgtccgcct tccccgggtt cttcccgccc ctcgtgctga cgggcgcgga
     6661 tgtcggcgcc aaccccggcg agttcggccg gcaggcctac accgatggcg gcgtcttcga
     6721 caacctcggc gtccgcatgt tcggcctgct ggaacggcca ctcctggcgg aaagtcccct
     6781 gtcgcgcgag gacttcatcg atttcccggc gttcgtcgag gcgctgagag aggccagcaa
     6841 gtccagccag gacacaccgc tgggccggtt cgcgcagatc ctggtggcgg cgtccaggcg
     6901 accggacccg ttaatgctca cagtcgccgg cacgtcgagt ggggcaggat tccagcctgt
     6961 cgccgggcct ggaccggttg gaagcccgcc ccactcgaca cccccaccgg gtgccagcaa
     7021 gggcgacggt gaggcagtct tgttgtccat cctctgggac ctcatgcgcc attatccctt
     7081 tcaccgcgag ccgctctttg ccggactgaa gcccctggac cccgaagcgg aagccctgct
     7141 tcatgacagc cgtcccggcg ggcgggatcc ggctgacagt gatcagctct ggttgaatcg
     7201 acacctgatc gaagccgcat tccggcaaac cacgggccgt gcctgctttc gcagactcca
     7261 cagcggactg gacggcgtcc tggtcagcga cgtgggcatg ccgatctcgg tgcagggcaa
     7321 ccggcgcgcc ggtggactca tccggaccgc cctgcgcgcc accgacatcc tcatgcaccg
     7381 ggtctggcaa ctggagatcg aaacgttcca ggacacgccc ggcttcgtct tcgccccgat
     7441 cacggaggtg gtcgaacacg ccgaagaccc gacggcctta caccccgaga tccagcgaca
     7501 ggccgccaag atccgcacgg acctcgatcg cttctccctg ctggaaatca gcagcttggt
     7561 gcgacacggc tactgtgtgg gacgcaaggc gtgccgggcg cgccccgacc tgttcggctc
     7621 ggagttgcca aacgacgccc cctgggatcc gatccccgga ccgcgcgacg cagttcctgc
     7681 ggccccggtg gccacgcggc cctcacgccg actccgctcc ccactgctgg gtcgaggggt
     7741 ggggggaaag gtggagccgg cggcggccgc agtcgaggcg cggacgttgc aagcctcggc
     7801 cctccggcgc atctggagta ccctgctgga ccccctggac tggatgacct acgtctacgt
     7861 gccactgata gtccccattc tcatcttgct gccctacttc tgcgtcaagt actaccagcg
     7921 gtcccaccgg atgaatcaac tcgtcgaatc gctttcccac ggcagtccgg attcgcagca
     7981 gatgagcctc ttgctcgagg gtccgatgaa gccctggatc ggcgaagccg ccgaggaagt
     8041 tcgcaacctc gacgagccgg actacaaggg tttcgagatc ctccaggact cgcgcatgct
     8101 tgacctacga aagtggaccc caaccgcctc cggggagggc gcctccagct cctcggtata
     8161 tggttatcgg cggttgaagc tcctcaaaca gcccgagaac accgggaaca acctcttccg
     8221 cttccgcctg ctcccaacca gtcccctggc acaagtgcgg ttcccgcttc agcaactgcg
     8281 gccaagactg cggaagagtt acgtggaagg cgcggtcccc ggcgagaaat cgtgtctctg
     8341 ggaggcgagc gtggatttcc agcgagtgcc ggccggcgat tacgtggacc tcacctatga
     8401 acatctctct ccgggagagt tcctgcagca cagtgagggt tcggacacgg tctcctcgga
     8461 gatccgggcg aagaccgccg aactgacccg gtggatcctc ttgcccgaag ggaaagagta
     8521 ccggggtttt cgcacgatcc ggtacaagac gggaaagccc gagacggcgg aagccgtgaa
     8581 gatcgtcacc gaatacctgg cggaggatta cacgatcctc gcctttaaat tgctgctgct
     8641 ggagcccggc tacacctacg acgtcacctg gtattacgag tagggactcg cccggccgga
     8701 ctgcccggga gtgaatggca gacttgcgtc cacgctctcg cggatcgacc gacagtagcg
     8761 atccagatcg aggtcgtctc tctcgcgacc gaacggctct tcgacgacgg aatcgatcac
     8821 ctccacgccg aagaggaaga agcacaccat caggatcact ggtagactcc aggcgccgat
     8881 gtccggcacg gtgagccagg gcgcggccag cacgttgagg accaggccgg cccgcagcaa
     8941 cccgacgtag gtgggggatg ggggcgtggt ccggatcttc tcgcacccgc cgcagacgtc
     9001 gagcagcccg cgcaggtggg cgtcgaggac ccagaggacc gcctggtcga tcgacgtggc
     9061 cgtcgcgttt ccagcccgcg acggcggcga atagccggcc cgcgagataa agcggcacgt
     9121 gctgcggatc ggcctcctcg ccctcgaacc ccggcagctc ccacacgcgc agcggctcgt
     9181 cgcgcagatg acgcttcagg gcctcggcga aggccacgag caagtcggcg actcgcgagc
     9241 gggccaggac gtcggccagc aggaagggag cacacttggc ggccaggttg cgccgcctcg
     9301 gcgaagggcg gcctttatgg gcggttcacc tcggccactg ccgaagacgt cgccttatca
     9361 accgaaccgc ctgttgcgct gcgcgcgtcg gtccgacgac accgacgcat cgagcacgag
     9421 atttcggcgc gtcggtcgat ccgaccgaag cgcgcaaggc gaccgtccga cgcttcggtc
     9481 gatccgaccg aagcgcgcaa cgctccggtc ctttcacagc ctctatggag tcgatggagg
     9541 gaccgcgcga gcagtcacca aattgccgcg accccccgtg cgaccgcggg accggccgac
     9601 gacatatctg ccgaaccgcc cgagggccgc ccaagcggct gatgcgaccc aagggggctg
     9661 tcgtcttggc tcggaggacg gtcctgcggt cctctttccc ttgggtccgc cccaccacga
     9721 ttctgccggt gtgtgtggcg tcgacgctca aggggaacga agaatcccgc ctgtcggcgg
     9781 ctcacctggg gtctggatcc gggagtgcag tcctcggaac ggtcacacgg atcccgaagt
     9841 ggcccagctc ccagattccg cctttcctcc caccgccgat gagcggagcg acgactcggc
     9901 gtcgaccccg acctccgcga acatctcgcg ggcaaaggcc atacccgcgg gagagagcga
     9961 ccggtcggcg agccagacga gccccgtgtg gaaagtcagc ggcggcgcgc cgatatcgag
    10021 cggcacgaag tcgcctcgcg cgagttcgac ccgcacctca tcagggtgga tcaacgtgac
    10081 ggcatcactc tggcggacca tcgctttgat catggtgacc gaggtggtgt tgacacgccg
    10141 tcgaaagccg cgcgagggag tttccgcggc ctccagccag cgctggatcc ggggcgtcag
    10201 ctgggggctg atcatcacgt actggcacag ggcagaacgg gacggcgacg cttgccccgt
    10261 gaggggatgc ccggcacgac agatccatat cccgcgctgc tccgggaact cgacgacgcg
    10321 gaactccttg cggccgcgga cttgctcgat gctcgcgacg aacaggtcga cacgccctgt
    10381 gtgaagcagg tccgggagac tctcccacgg ctcgacgtgg atcgagatgc gtaaccgggg
    10441 gtatcgctcg agcaaccggc cggaaacctg ccccatccag gtgtcggcgg tgtagggccc
    10501 cacgccgacg gccagctcac cggcatccag ggcggcggtg tggccgatgg cccgggtcat
    10561 cgcctcctgg gagtcgagca tcttgcgggc cgtgtcgacg acgatcgcgc cgaagacggt
    10621 gggcgagatc gtctggccat gcctgtcaaa aagccgctgg cccaaatctc gctcgatctg
    10681 gaagagactc ttggtcagcg cgggctgtga gatgccgagt gcctcggacg ccttgcggaa
    10741 gctggcgtgg tcggccaggg ccagcacgtg ccggtatcgt cgcaattcca cgcggtccct
    10801 ccccggttat cggggcgata acctctggaa aataaggatg cgaacagtct tgccatacca
    10861 ttttttgcgc ataatgtacc aagtatccat ggatccggcg agaaccggcc ggtgaggtca
    10921 gccgccaagt acgggcatca cccgggcgtt cggtttgcgc ggccgaggag atgacccctc
    10981 cattgtaccg cagcacacga cagcgccgac tgccggcgca gggcaaaggc atctgccatg
    11041 acttcagtac tttcgcgcgc ttgccttgcg gggatgatga tggttctctt ggccctggca
    11101 tacgccggtt gcggtccgtc gtctccctcg gacgaaccaa agaccgagaa tcaggcccgt
    11161 gatgaaaaga agattcagga gctgtcaaga acgggatata acttcagcga gatccgatcg
    11221 atcatgaaag gggagcagcc gaagcctcga cccaagaaaa agacggggct cgcaaggcgt
    11281 tagcgcgccc tgttcagact gactcgctcc tgtagcacaa accccttcca acgccggcct
    11341 tcgaccagtg ggtaacgctc gtgagggcgc ttgcaatggg tctgggcccg gacgagcctc
    11401 gaatgccgag ggacatgaga ccgagcggca cattgtcgat tcgtcctgta gagacccata
    11461 caagcctgtg ttactttccc gccccaccct accagcctat gacagaaggt gctcgaacac
    11521 atgaaaactc gtattcgacg gcgtgcgttc acgctgattg agctcttggt ggtaatcgcc
    11581 atcatcgcgg tcctgatcgc cctgttgctt cctgcggtgc aggccgcccg cgaagctgcc
    11641 cggcgcatcc agtgcacgaa caacctcaag caggtcggcc tggcgattca caattatgtg
    11701 agtgcgaaca acagcctgcc ttccggcgtt gtgttcaaca ccagtgtcgc accatgctcg
    11761 gggagtggct tcggcaaaag ttgccagaac acgacctggt ttgtccttac cttgccgttc
    11821 atcgaggggg ggaacctgtt caacacgttc aatttcgcga tcggttccga gggtccgatg
    11881 gccctgaccg ggtatcccta cggctacgcg gtcaacagca ccgttctgac cactcatatt
    11941 ggcttcttcc aatgtccgag cgacacagag aaaacgtttg acgtcgcctc gtcgactgtg
    12001 ggagcggttc tttccagtgc tcccaagctt tcagtcacga agggtaacta tggcgtgaac
    12061 tggggaaata ccgattacgg ccagggccag cggggcgata gctggttcgc ttcgaacccc
    12121 tcgacccacc gaccggcgcc gttcgggtac aaccaggggg caacggggcc gctgagggcg
    12181 accatcgcct ccttcaccga cggcacgagc aacacgctct tgctcagcga gatgctccag
    12241 ggagcgaatg acgacatccg cggcttgctc tggaccgact ttcccggctc gcactcgttc
    12301 gtgacccggc tcgcgcccaa cggcaacgtc gacttctggg cggggtccgc ttacctcgac
    12361 ccggccggcg gggacaccat ggacaacctg ccaacctatg gcacctcgga gcccgggacg
    12421 agcccggcga acattgccaa cggcgacctg tgcgacaacc agcccggttt caacctgccc
    12481 tgcttctcgc aaggcaagga agggggaagt ttcgccgcct ctcgaagccg tcatcccggc
    12541 ggcgtcaact cgctcctggc cgacggctcc gtacgattct tcaagaattc gattaacctg
    12601 cccacctggg tcgccctggg ctcgatggcc ggaggggaag tcatcagcgc cgactcatac
    12661 tgatcgccga atctgaaggc ggtatacgaa cggcgacgaa cgctcgggag gattcgatgg
    12721 gacggtgcgg ccggcgatgg ccggaacgtg gcatcatccg agggctcggc gaaaggggag
    12781 agaagcatgg tgtcgactcg gtcgaggggt atcggcgtgc tgacggtggc gatggcgatc
    12841 atggccgcgg ggcgaccggg catcgccctg ggcgcttccg aaggcgacga gtttcgggtc
    12901 ggggcggccg acaaggagat cacgccgccc ccggggattc ccatgtgggg ctatggcccg
    12961 cggcacgaca agcccagcga gggcacgctc gaccccctca tggccaaggc gatcgtgatc
    13021 gcggcgggcg acgacaaggt cgcggtcgtc ggtaccgacc tcggccgcgg gccgaccgag
    13081 gccatgatgg cgatcat
//
LOCUS       DATAQL010001011         6292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4224258, whole genome shotgun sequence.
ACCESSION   DATAQL010001011 DATAQL010000000
VERSION     DATAQL010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4224258"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..982
                     /gene="ssb"
                     /locus_tag="VHS97_17505"
     CDS             317..982
                     /gene="ssb"
                     /locus_tag="VHS97_17505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX3450054.1"
                     /translation="MCYRSLWGTTVYYHSRSAGTNVGGKVTTMADLNKVFLMGRLTFD
                     PELRRTTGGTAVTELRLATTRSWSGRDGERREETLFIGVTVWDRQAENCCQFLRKGSS
                     VHVEGSLKMDTWDDKNTGEKRSKILVHADRVQFLDGRRGDSTSGMGAGPDDDYTAGPQ
                     TARDPSARRGGASAPARGNAGAGESRGSSNGSGRPAYQAPSSSESPVGSAADDAEEDD
                     IPF"
     gene            1240..1884
                     /gene="rplI"
                     /locus_tag="VHS97_17510"
     CDS             1240..1884
                     /gene="rplI"
                     /locus_tag="VHS97_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX3450055.1"
                     /translation="MAKTTDSKAPKAKAGTAAIKAKGQSKASETSKMKTATAGPPRQP
                     GVERRKNHPSRAKHGHIQLILTQAISKLGQPGDLVTVRPGFARNYLVPQGLATFATQN
                     NLRMVEKHRQRLKELEQARRADLQNLAAQLAQRSLTIEANANAEGHLYGSVNADQIAE
                     SLRNEGFPIQGENVRIEGPLKELGLYTIRIQLAQEIHGEVKLWVVPTHTEEASG"
     gene            2032..3390
                     /gene="dnaB"
                     /locus_tag="VHS97_17515"
     CDS             2032..3390
                     /gene="dnaB"
                     /locus_tag="VHS97_17515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX3450056.1"
                     /translation="MVSDRLPPQHLEAERGVLGSILLDNEVMHDIVVFLRAEDFYRDA
                     HQVIYAAIRELYDKAKGIDAVTLAEELKLRGLFDQVGGDDTLTEIVTSVPHAANGKYY
                     AEIVKEKATSRQLIESATEIIRDGYSNQFTAQELLEGAERKIFGIAEDQIRGETLELK
                     DVVKQAMDLIGIRADSGGHAVTGLATGLIDLDDITGGFHQGQLIIVAARPSMGKTALA
                     LNICDHCGVNLKVPVLFVSLEMGHIEIGERMLCARSRVDGHKLRTGMGMALQEMRKLS
                     TAFNDMSKNAQIYIDDTPARNMMQIAASARRIKRRSQIGLLVVDYIQLVDSDDSRDSR
                     QEQIAKISRRLKTLARELKVPVVALSQLNRAVESREDKRPRMADLRESGAIEQDADMV
                     LLLHRPEYYDANDQPGMAELIVAKNRNGPTGNIKLTFLKNLTRFENFGAVAEPFDEGS
                     PF"
     gene            complement(3408..5234)
                     /locus_tag="VHS97_17520"
     CDS             complement(3408..5234)
                     /locus_tag="VHS97_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450057.1"
                     /translation="MSLWVATSATALSMTVEFVRESGVSARTILKGQTERLAERPEPG
                     AQAFAWTIWALLSVAAVTFVAQYGTDVPVWDDYAIVSALVGDRQVSPEWLWEQCNEHR
                     IALPKLILFHAERLAGNDVRAGMFLTVGTLIALAAGLLVASSRLEGGIRPSDALFPLL
                     LLNVGQASNFLWSIQFIHVLPTALATTWLILIVSRPTWPTFWMILSTGILMGLLPLCG
                     GTGLMFVPGLALWMLGVAVEEGRSTRPGHRSRALTAALAAVPGLVATAFYFRGFRKEP
                     HPEAIGGILDGGRTAIQFLTGGIGIPAPWLWPWSGAVVLGLTALTLAFLGRAWAFRPG
                     ERSRIFGLAAFLAAILALGAAVGWGRGWAGSQAGFQDRYITMATPLFCWLAFGCRLYA
                     PPALGRLVPNALFTALCVVAWPNTQTGIEHGRKGSEQARALTSDLSAGVPAYQIVRRY
                     TPYLHPNQDEIARLMPKLRNARVGSFKALRDSPTFRETLLPIAPSHVSLARWDGNMAH
                     VTGVDPQITFTLARPRPVAGIRISYSHSNGQGAPARFNLTWKRPGQSSYAPAQRYANW
                     NLPTGEGRETTVWIDDVIEVFRIQPDNQPCEFRIDKITILEL"
     gene            complement(5326..6111)
                     /locus_tag="VHS97_17525"
     CDS             complement(5326..6111)
                     /locus_tag="VHS97_17525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3450058.1"
                     /translation="MITKWSEGDADVNMDEDILVELSRTIHRHPWWRARANLTIALLK
                     RLGVNRSARVLDAGCGWGVTLGLLERCGYRADGLDISRRALDQLDRPGRLLIEADLAQ
                     PLADRFEPYDAVLALDVIEHLDDDRVPVANLGQLARPGGVVVISVPALPELFTEFDAI
                     QGHRRRYLPESLRSAFVGSGLEVERLFWWGAWLVPLLRRQRRRTRSQPGATASEIYSR
                     YLHLPPWPLPWLLRVAFAVEQPPALAGALTTGTSLFAVGRKLE"
     gene            6110..>6292
                     /locus_tag="VHS97_17530"
     CDS             6110..>6292
                     /locus_tag="VHS97_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450059.1"
                     /translation="MAQGSPSSPADEPFDRQRSVQSTGCQITLSHDLAALRLAHTVIV
                     AGIENIAAPVPPDVTSA"
BASE COUNT         1378 a         1911 c         1895 g         1108 t
ORIGIN      
        1 tgcggcttga gcgacatcat cctgagagag ttggtgctca aggtgcatcc caagctggct
       61 gaccacctgg tcaatcaggc gatgacatca acgccgaatg tggacgccga gaacggcatc
      121 ggtgaggatg agctcgacga ccggccgcgc cgtcgtcgtc gcgacgactg agccggcacg
      181 ctggcgaagg ctttcgtttt cccacgaatt gcctcgcctc cgctcgccgg cgccggcttg
      241 ttgactcgct atcgtctcca cagtttcgcg gcatctattt tcactggcaa actggactct
      301 tgtcgatcga gcggaaatgt gctaccgttc cctttgggga acaaccgtct actatcattc
      361 gcggtcggca ggcacaaacg ttggcgggaa ggtcacgacg atggctgact tgaacaaggt
      421 atttttgatg ggtcgtctga cattcgaccc tgagctacgg cggacgacgg gcgggacggc
      481 ggtgaccgag ttgcgacttg cgacgactcg atcgtggtcg ggccgcgacg gcgagcgccg
      541 cgaggagacg ctcttcatcg gcgtgacggt gtgggatcgc caggccgaga attgctgcca
      601 gttcctgcgc aaggggagct cggttcatgt cgagggctct ttgaagatgg acacctggga
      661 tgacaaaaat accggggaga aaaggtcaaa gatcctcgtg catgccgacc gcgtgcagtt
      721 tttggatggc cggcgcggcg attcaacgag cggcatgggc gccggtcccg atgatgatta
      781 cacggcgggc ccgcagaccg ctcgagaccc atccgcgcgg cgcggcgggg cgtccgcccc
      841 tgcacgtggt aatgccggcg ccggcgaatc ccgaggttcc tccaatggtt cgggtcgacc
      901 tgcgtaccag gctccgagct cgtccgaaag ccccgtcgga agcgctgccg acgacgccga
      961 ggaagacgac atcccgttct gaatagcgat cggaaacagg gcttctagca tgcccgatgg
     1021 acggagaatc tttttcccgg atagcatgac gtaatggccc gggcgcacag atgaaccgct
     1081 tggtaaaagc cagccgccag attagaaacg taggaaaagt cgagcgcaga gaaacggaag
     1141 ttggttttgt gtgggcgcgg ctccgcccga gccaaaccag gtgcggggaa gcgacggagc
     1201 cttgtcgtcc ccattttgct gtagaagatt gggaaactca tggccaagac gaccgattcc
     1261 aaagccccca aagccaaagc cggtacggcc gctataaagg caaaaggcca gtccaaggcg
     1321 tccgaaacca gtaagatgaa aaccgccacg gccgggccac ccaggcagcc gggtgtcgaa
     1381 cggcgcaaga atcatccgag tcgggccaag cacggtcaca tccagctcat tctgactcag
     1441 gccatctcga agctcgggca gcccggagac ctcgtcacag tccgcccagg attcgctcga
     1501 aactatctgg tcccacaggg cttggcgacc tttgccactc agaataatct gaggatggtc
     1561 gaaaagcatc gtcagcgact gaaagagctt gaacaggccc gacgtgctga tcttcaaaac
     1621 ctggccgctc agctcgccca gcgctcgctc acgatcgaag ccaatgccaa cgccgagggt
     1681 catctctacg gctcggtcaa tgccgatcag atcgcagaat cgctacggaa cgagggcttt
     1741 ccgatccagg gcgagaatgt ccgaatcgag ggacccctca aggagcttgg tctctacacc
     1801 atcagaatcc agttggctca ggaaatccat ggcgaggtca agctctgggt ggttccgacc
     1861 cataccgagg aagcgtcggg ctgaaacggc cgccgaatgc ggtattgaca gtgcctgacg
     1921 cgacgcaagc ggtgccggcg gactgtggtg ggcgagaggc cgaaaatcgc ctcgacgctc
     1981 gctacctcgg ctagaacggc taaaggcgcg atctcaaggt tggaatccgg catggtgagc
     2041 gaccgccttc ccccccaaca ccttgaggcc gaacgcggag tgctgggaag cattctcctc
     2101 gacaacgaag tcatgcacga catcgttgtc ttccttcgcg ccgaggattt ctatcgcgac
     2161 gcccatcagg tgatctacgc ggcgatccgt gaactttacg acaaggctaa agggatcgat
     2221 gcggtaactc ttgccgaaga gttgaaactg cgaggcctgt ttgaccaggt gggtggcgac
     2281 gacacgctca ccgagatcgt gacgagcgtc cctcacgccg ccaacggtaa atattatgcc
     2341 gaaatcgtca aagaaaaggc cacaagtcgc cagttgatcg agagcgcgac cgagatcatt
     2401 cgtgacggat actccaacca atttacggcc caagagcttc tcgaaggcgc cgagcgcaag
     2461 atattcggga tcgccgagga tcaaatcagg ggagaaacgc tcgagctcaa agacgtagtc
     2521 aagcaggcga tggatttgat aggcattcgg gccgattcgg gcggccacgc cgtcacgggt
     2581 ctggcgactg ggctgatcga tctcgacgac atcaccggcg gttttcatca gggccagttg
     2641 atcatcgtgg cggctcggcc gagcatggga aaaaccgcgc tggcgctcaa catctgcgat
     2701 cattgtggag tgaacttaaa ggtccctgtg ctgttcgtca gcctggaaat gggtcatatc
     2761 gaaattggcg agcgtatgct ctgcgcgcgc tctcgggtcg acggccataa acttaggaca
     2821 ggtatgggca tggcgctaca agagatgaga aagctcagca cagcctttaa cgatatgagc
     2881 aagaatgccc aaatctacat cgacgacact ccagcgcgca atatgatgca gatcgcggcc
     2941 agcgcccggc gaatcaaacg gcgaagtcag atcggcttgc tggtggtcga ctatatccaa
     3001 ttggtggact ccgacgactc gcgggacagt cgccaggaac agattgccaa gatcagtcgc
     3061 cggctcaaaa cgcttgcccg tgaactgaag gtgccggtcg tcgccctctc ccagctgaac
     3121 cgagccgtcg agagccgcga ggacaaacgc ccccggatgg ccgatttacg cgaatcgggc
     3181 gcgatcgaac aggacgccga catggtgctt ctccttcatc gtcccgagta ttacgacgcc
     3241 aacgaccaac cgggaatggc tgagctcatc gtcgccaaga atcgaaatgg gccgactggc
     3301 aacatcaagc tcaccttcct caaaaacttg acacggtttg aaaactttgg cgctgtggct
     3361 gaaccgttcg acgaggggag cccgttctga gtccaagcga gctcgcctca aagttccagg
     3421 atggtaatct tatcgatgcg gaattcgcac ggctgattgt ccggctgaat acgaaacact
     3481 tctattacgt catcgatcca tacggtggtt tctctccctt ctccagtggg caagttccag
     3541 ttcgcgtaac gttgcgccgg cgcgtagctg ctttggccgg gtcgcttcca ggtcagattg
     3601 aatcgcgcgg gcgcaccctg gccattactg tgagagtagc taatgcgaat gccagcgact
     3661 ggacgcggcc gcgccagcgt gaacgtgatc tgggggtcga cgccggttac gtgcgccata
     3721 ttgccgtccc aacgggctag cgagacatgg gatggcgcga tcggcaacaa cgtctcgcgg
     3781 aaggtcgggg aatcgcgcag cgctttgaat gagccgactc tcgcgttgcg caatttgggc
     3841 atcaatcggg cgatttcgtc ctggttcgga tggagatacg gagtgtatct ccgcacgatc
     3901 tggtaggccg gcactccagc gctaagatca cttgtaagcg ctcgcgcttg ctccgatcct
     3961 ttccggccgt gctcgatgcc ggtttgggta ttcggccagg cgacgacaca aagcgccgtg
     4021 aacagggcat tcggcaccag ccgaccgagg gccggcggtg cataaagccg acagccaaaa
     4081 gcgagccagc aaaaaagggg ggtcgccatg gtaatgtagc ggtcttgaaa cccggcctgg
     4141 ctaccggccc agcctcgtcc ccagccgacc gccgcaccca gcgccaggat ggccgccaga
     4201 aaggcggcca ggccaaagat acgagacctc tcacccggcc ggaacgccca tgctcggccg
     4261 agaaaggcga gggtcaacgc cgtcagcccg agcaccaccg ctccggacca tggccagagc
     4321 catggcgccg ggatcccgat cccaccggtc aggaactgga tcgcggtcct gccgccgtcc
     4381 aatattcccc cgatggcctc agggtggggc tccttccgga agccgcggaa atagaaggcc
     4441 gtcgccacga gtccaggcac cgccgccaaa gcggccgtga gggcgcgact ccggtgtccc
     4501 ggacgcgttg atcgtccctc ttccacggcc actccgagca tccaaagcgc cagtcccggg
     4561 acaaacatca gcccggttcc gccgcagagc ggcagtagtc ccatcaagat tcccgtagac
     4621 aagatcatcc agaaagtcgg ccaagtcggt cgggacacga tcaggatcag ccacgtcgtt
     4681 gccagcgcgg tcggcagcac gtggatgaac tgaatgctcc acagaaagtt gctcgcctgc
     4741 ccgacgttca ggagcaagag cggaaagagg gcgtcagaag gtcgaattcc gccttcgagc
     4801 ctcgaggacg ccaccagcaa gccggcggcc agagcaatga gcgtgccaac agttagaaac
     4861 attcccgcgc gaacatcatt tcccgcgaga cgctcggcat gaaaaaggat cagcttcggt
     4921 agcgcaatcc gatgttcatt acattgctcc cacagccatt cgggactgac ctgccgatcg
     4981 ccgacaagcg ccgagacgat ggcgtaatcg tcccataccg gcacgtcggt gccgtattgg
     5041 gcgacaaatg tgactgcagc caccgacaaa agggcccaaa tagtccaagc aaatgcctgg
     5101 gcgccaggtt cgggtcgctc agccagtcgt tccgtctgtc ccttaagaat cgtgcgcgca
     5161 cttacgccac tttcgcgcac aaactcaact gtcattgaga gtgcggtggc gctcgtggct
     5221 acccagagac tcatcactcg ccccgtcccg tgaccttgcc caaagacctc acgatgcgtc
     5281 tcgacgctcg gacggccgca tctactttgg aatgagattg atcattcact caagctttcg
     5341 accgaccgcg aatagcgaag tccccgtggt cagtgccccg gcgagagccg gaggctgctc
     5401 gacggcgaag gctacccgga gtagccatgg taaaggccat ggcggaagat gaagataacg
     5461 actgtagatc tccgatgcgg ttgctcccgg ctgcgaacgg gtccgacggc gttgccggcg
     5521 caagagcggt accagccatg ccccccacca gaacagccgc tcgacttcca ggccgctacc
     5581 cacgaaagcc gacctcaagc tctcggggag gtagcggcgc ctgtgcccct gaatcgcgtc
     5641 gaattcggtg aagagctctg gcaacgcggg gacgctgatc acgaccacgc cgccagggcg
     5701 agccagctgt ccgaggtttg caacgggtac acggtcgtcg tccaggtgct cgatcacatc
     5761 aagcgccagc acggcgtcat agggctcgaa acgatcggca agcggttgtg cgagatcggc
     5821 ctcgatcaac agccggccgg gccgatcgag ttgatcgagg gcgcgtcgtg agatatccag
     5881 accatcggcc cgatagccgc atcgctccaa aaggcccaag gttacgcccc agccacatcc
     5941 ggcgtcgagc acccgcgcgg accgattgac ccccagtcgc ttcagcaagg caattgttaa
     6001 attggcacgg gcccgccacc aggggtgacg gtggatggtc cgggacaact caaccaagat
     6061 atcttcatcc atattcacat ccgcatcacc ctcggaccat tttgtgatca tggctcaggg
     6121 atccccctcg tctccggcgg atgagccttt cgatcggcaa cgctcggtgc agagcaccgg
     6181 ctgccagatc accctgagcc acgatctggc ggcgctgcgc cttgcgcata cggtcatcgt
     6241 ggccgggatt gaaaacatcg cggcgccggt ccccccggat gtgacctccg cc
//
LOCUS       DATAQL010001012         5667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4834543, whole genome shotgun sequence.
ACCESSION   DATAQL010001012 DATAQL010000000
VERSION     DATAQL010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4834543"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VHS97_17535"
     CDS             <1..381
                     /locus_tag="VHS97_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX3450060.1"
                     /translation="LPPVMGEIASHDQGIVILAGVTGSGKSTTIAAMLNYVNTRERCH
                     IVTIEDPIEFTFKDDKCIINQREVGIDVIDWNTALKHSVTRPGHYPGRRNARPRDVQR
                     RHARRRNRPPRVWNDPRLERSVDD"
     gene            311..769
                     /locus_tag="VHS97_17540"
     CDS             311..769
                     /locus_tag="VHS97_17540"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX3450061.1"
                     /translation="MHAAETGHLVFGTIHASSAPSTISRILDLFPKDMHSAMRQNLAF
                     NLKGIVAQKLLPTTKDWAASTGQTRVPLVEVMRMNPTVRKLILQEEDIKLAQAIRLGK
                     EEGMVDFTESLRQLVVAEKIERATAFEVAPNPELLRMALKGITLPEQGIL"
     gene            985..2730
                     /locus_tag="VHS97_17545"
     CDS             985..2730
                     /locus_tag="VHS97_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX3450062.1"
                     /translation="MFRLSLAFFAFFVVVFGAALAHAAEPLPAILAQIPNMTRGTGMY
                     LNLFKFVPVVVIYILWAWTTDWVEHDTKDLNNLRFGVWNSVVFFSGALGLVLALAIPI
                     YFLGLGLLLLAYFIPLFTYVYARNQTVPDDQKVLTPYHFGELANDILHKLGMKAIFNR
                     DVGTVDRAGPPITFIGKTQGSTKEDPSRVRQAEESRSFMGAKELVYDAVLRRATDIHL
                     EPTTEQLSVRYRIDGILHAAEPFDRPTGDAVVNVFKVLSAMDISEKRKPQDGSFGAKL
                     QGRDLDFRVATSGSKAGEKLVMRILDNSAGITKMEDLGMRPKLVEQVRGLVTQPHGMF
                     LCCGPTGAGKSTTLYASLREIDRYQKNIITVEDPIEYHLDNITQMEVNTKAGQTFAAA
                     LRSILRQDPDVIMIGEIRDQETASIACQAANTGHMVFSTVHSNDAVTALFRLLDLGVE
                     PFMIASALSGVMGQRLVRILCDMCKEPYKPKPEFLKKANLPVEKVDVFYRRPENPEQV
                     CQQCGGTGYFGRTGIYELLVLTETMKDMIRENPSINKIKAEARKNGMIYLQEDGLRQV
                     IQGRTSIEELLRVVK"
     gene            2820..3542
                     /locus_tag="VHS97_17550"
     CDS             2820..3542
                     /locus_tag="VHS97_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX3450063.1"
                     /translation="MTTIIDLIIVGFVLGLTYVLTSEGLWGAALMFFNVLFGAMIAFN
                     FYEPLAKLIDSTGIGWGFSDTLCMLGLFCVSTALLKMTTETIAPAMVRFPAPVYHVGR
                     LIFGAAGALVTVAFVLLAFHTAPVHKKIFTAVTYNSKPPFGLGLDHQFLGFFQYQTGA
                     VFTMLGSGQRDPHHQYGHGGAVKMFDPRARWLLDHQDARPYGDEMVLTPETAAPEAGG
                     AQQPGGAPGPGGQRGPGGRPPG"
     gene            complement(3598..4146)
                     /locus_tag="VHS97_17555"
     CDS             complement(3598..4146)
                     /locus_tag="VHS97_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450064.1"
                     /translation="MLVSRSCIPTALALVVGVCIGLALASLRPTPLHAGAGDRSGESI
                     VATGPVLVRYDEAAKGPIALDAVYLLDYKRGRLLASVPAFQQTNTATRLIESFSERDL
                     VADFKLDLETGPRPRFLMTTGSLGPYNDGWAPLYVFETNTSQVAIYRMQVQLTIGKTS
                     RPRFDLTEVRSFAKTGATNPTP"
     gene            4347..>5667
                     /locus_tag="VHS97_17560"
     CDS             4347..>5667
                     /locus_tag="VHS97_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450065.1"
                     /translation="MSCTVVLVGLSLLVQVPPPALGATTARELEAARRSIVVNETAQV
                     SRLAEQLAGGGDPNAAQLVRERIPRPVERDGPTRIMPLAEVVQARPEEKGGEPWRARL
                     KEIEARSSTELFNLAQRAAKSDPPSFALASQCLRAVLERQPDHREARRLLGYVPHDGG
                     WARPFAVNKLRNGFVSHPIFGWVLSDWVPHLDRGELPAPPGKGQNKTRWLSANDANGL
                     RANWSPPWYIYTEHFEIQTNVTLAEAISFGRRLEAFHDVFMALLVDILGENLPLVRRF
                     KSPSMNGEPEYKLHSVFYFGSKDEYVDFLTPRYGPKVAEESLGYYDPPKSGANKRAPA
                     YFFRDPNGQLPMTATLYHEVSHQLLFETAGSNGYTKNVGNYWVFEGLGTYFETVEPQP
                     DGSIEVGGRVGRRLEEAIRSLALLGRTIPLEQFVNYDEAAFSHDDSAR"
BASE COUNT         1155 a         1746 c         1684 g         1082 t
ORIGIN      
        1 ctgccccccg tcatgggcga gatcgccagc cacgaccagg ggatcgtgat cctggcgggt
       61 gtcaccgggt caggcaagtc gacgacaatc gctgcaatgc tcaattatgt caacacccgc
      121 gaacgctgcc acattgtcac catcgaagac cccatcgaat ttaccttcaa agacgacaag
      181 tgcatcatca atcagcgcga agtaggcatc gatgtcatcg attggaatac cgcgctcaag
      241 cactccgtga caagacccgg acattatcct ggtcggcgaa atgcgcgacc gcgagacgtt
      301 caacgccgcc atgcacgccg ccgaaaccgg ccacctcgtg tttggaacga tccacgcctc
      361 gagcgctccg tcgacgatta gccgaatcct cgacctgttc ccgaaggaca tgcactcggc
      421 gatgcggcag aatctggcgt tcaatctgaa ggggatcgtc gcccagaagc tgttgccgac
      481 caccaaggac tgggcagcct cgacggggca aacccgcgtt ccgttagtcg aggtcatgcg
      541 gatgaacccg actgtccgca agttgatcct gcaagaggaa gacatcaagc tggcacaggc
      601 gatccggctg ggaaaagaag agggaatggt tgacttcacc gagagcttgc ggcagcttgt
      661 ggtggccgaa aaaattgaac gggccacggc gtttgaggtc gcgcccaatc ccgaattgct
      721 gcgcatggct ttaaagggga tcacgttgcc cgagcaaggc attctgtgac gtctgcgttg
      781 cgagcggatg gccaggagcc tccagacacc gcgagcggcc ggcgtccctg ggagatggcg
      841 ctcgaggccg cttcgagcgg cctgtttttg ctaatcgtga cgagaagaat taccatcgat
      901 tgagtcaacg ccggcgtcag tcgagatcgc gaaggggcgc cgttcgtttt tgtccagagg
      961 catcgctccc aagaaaggtc agcaatgttt cgcctctccc tggcgttttt tgccttcttc
     1021 gttgtcgtct tcggcgccgc cctagctcat gccgccgagc cgctgcctgc cattctggct
     1081 cagattccca acatgactcg cggcacggga atgtacctga acctgttcaa gtttgtgccg
     1141 gtggtcgtca tctacattct ctgggcctgg acgaccgact gggtcgaaca cgacaccaag
     1201 gatctcaata accttaggtt cggcgtgtgg aacagcgtcg tctttttctc gggcgcgtta
     1261 ggattggtcc tggcactcgc gataccgatc tactttctcg ggctgggact cttgctgctg
     1321 gcatacttca ttcctctctt tacgtatgtc tacgcacgca accagaccgt acccgacgat
     1381 cagaaggtct tgacgcctta tcatttcggc gagctggcca acgatatctt gcacaaactc
     1441 ggtatgaagg cgattttcaa tcgcgacgtg ggtacggtcg atcgcgctgg cccgccgatc
     1501 acgtttatcg gcaagaccca ggggtcgaca aaggaagacc cgtcgcgcgt gcggcaggcg
     1561 gaggagtcgc gatcgttcat gggcgccaag gaactcgttt acgacgctgt gctgcggcgg
     1621 gcgaccgaca tccacttgga gccgaccacc gagcagctct cggtgcggta tcggatcgac
     1681 ggcatcttgc acgcggccga gccgttcgac cgtccgaccg gcgacgcagt ggtcaatgtc
     1741 ttcaaggtct tatcggcgat ggacatctcc gaaaagcgca agccacagga cggttcattc
     1801 ggcgccaagc tgcagggccg cgacctcgat ttccgtgtgg caacttcagg gtcgaaagcc
     1861 ggcgaaaagc tggtcatgcg aattctcgac aacagtgccg gtatcaccaa gatggaagac
     1921 ctggggatgc ggcccaagct tgtcgaacag gtccgcggac tggtcactca gccacatgga
     1981 atgtttctct gctgcggtcc gaccggggcc ggtaagtcga ccacgctcta cgcctcgctg
     2041 cgggaaatcg accgctatca gaaaaacatc atcaccgtgg aagacccgat cgagtaccac
     2101 ctcgacaaca tcactcagat ggaggtcaac accaaggcgg gccaaacttt cgccgcggcg
     2161 ttgcggtcga tcttgcggca agaccccgac gtcatcatga tcggcgagat ccgcgaccaa
     2221 gagaccgcat cgattgcctg ccaggcggcc aacaccgggc acatggtctt ctcgacggtg
     2281 cactccaacg acgccgtaac cgccctgttt cggctcttgg acctgggcgt cgagcccttc
     2341 atgatcgcct cggccctctc aggcgtcatg ggtcagcggc tggtgcgcat cctctgcgac
     2401 atgtgcaaag aaccgtacaa gcccaagccc gagttcctca aaaaggccaa cctgccggtg
     2461 gaaaaagtcg acgtcttcta ccggcgtccc gaaaatcccg aacaagtctg ccagcagtgc
     2521 ggcggcaccg gctacttcgg ccgcacggga atctacgagc ttctagtgct caccgaaacc
     2581 atgaaagaca tgatccgtga aaacccatcg atcaacaaaa tcaaggccga agcccgcaag
     2641 aacggcatga tctatctgca agaagacggc ctccgccagg tcatccaggg ccgaacctcg
     2701 atcgaggaac tcttgcgcgt cgtcaagtaa ttcagcaacc gggggaggcg tctccctcca
     2761 tcccggagat aaaggctgca cgccatattc atccgcagga gggaatcgag aaattcatca
     2821 tgacgaccat catcgatctg atcatcgtcg ggttcgtcct gggtctcacc tatgtgttga
     2881 ccagcgaggg cctgtggggc gcggccctga tgttcttcaa cgtcctgttc ggcgcgatga
     2941 tcgcgttcaa cttttacgag ccactggcca agctgatcga ttcgacaggc atcggctggg
     3001 gcttttccga cactctctgc atgctgggtc tgttttgtgt gtcgacggct ctgctgaaaa
     3061 tgacgaccga gaccatcgca ccggcgatgg tgcggttccc ggccccggtt tatcatgtcg
     3121 gccggctcat tttcggagcc gcgggggcac ttgtcaccgt ggcattcgtt ctcctggcgt
     3181 ttcacacggc tccggtccac aagaagatct tcacggcggt cacgtacaac agcaagcccc
     3241 cattcggcct ggggctcgac catcagtttt tggggttctt ccagtatcaa acgggcgcgg
     3301 tcttcacgat gctgggatct ggccaacgtg atccacacca tcaatacggt cacggcgggg
     3361 ccgtaaagat gttcgacccg cgcgcccggt ggttgcttga tcaccaggac gctcggcctt
     3421 acggcgatga aatggtcctc acgccggaga cggcagctcc cgaggcgggc ggcgcgcaac
     3481 agccgggtgg agcgcccggg ccggggggcc aacgaggccc cggcggcaga ccacctggat
     3541 aaagccccaa agggctatcg ccaatcgtct cccagcctgc tgcctggcta acaggcgtca
     3601 gggagtagga ttcgttgcgc cggtcttggc gaacgatcgc acttccgtca gatcgaagcg
     3661 cgggcgtgaa gtcttaccga ttgtcaactg cacttgcatc cgatagatcg ccacctggct
     3721 ggtgttggtc tcgaaaacgt agagcggggc ccagccgtcg ttatagggtc cgagcgaacc
     3781 cgtcgtcatc agaaaacgag gacgcgggcc ggtttccaga tcgagcttga aatcggccac
     3841 caggtcgcgc tcactgaacg actcgattaa acgcgtcgcc gtgttggttt gctgaaaagc
     3901 aggaacgctc gcaagcagcc gacctcgctt atagtcgagc aagtagaccg catcgagcgc
     3961 gatcggcccc ttggcagctt catcgtagcg aacgagcacg ggtccggtcg cgacaatcga
     4021 ctcgcccgac cggtcgcccg caccggcgtg gagaggcgtc ggacgcaaac tcgccaacgc
     4081 caatcctatg cacacaccca ccacgagggc taatgcggtg ggaatacagg aacgcgaaac
     4141 caacatgatc agcgactcct ttcgcggccc tgaaaccctt cggtaagatt tgactttcga
     4201 gcgtcccaac agggcttctt ccgttcaatt cggccggcgc cttcatgctg gcctgggcgc
     4261 tcgggcgtgc attgtgcgac gatcaatgag ttgcgtccag cccaagaacg gaaaaggcca
     4321 aacgccgtat tgacagaggg aaagcgatgt cgtgcacggt cgtgctggtt gggctatcgc
     4381 tgctggttca ggtaccgcct cccgctctgg gtgcgaccac agctcgcgag ctcgaggctg
     4441 cgcggcggtc tattgtggtg aacgagacgg cccaggtgag caggctcgcc gagcaattgg
     4501 caggcggagg ggatccaaat gccgcccagc ttgtgcgcga gcgaatcccc cggccggtcg
     4561 agcgggacgg acccacgcgg atcatgccgc tcgccgaagt ggtgcaggct cgtcccgagg
     4621 aaaagggggg tgagccgtgg cgtgcccgac tgaaagagat tgaagcgcga tcgagcaccg
     4681 agcttttcaa cctggcgcaa cgggcggcca agtccgaccc gccgtcgttc gccctggcca
     4741 gccagtgcct gcgagcggtg ctcgagcgac agcccgatca tcgcgaggcg cggcggttgc
     4801 tcgggtacgt gccgcatgat ggcggctggg cgcggccatt cgccgtcaat aagcttcgga
     4861 acggcttcgt gagccatccg attttcggct gggttttgtc agactgggtc ccccacctag
     4921 atcgcggcga gctgcccgcc cccccgggaa agggacagaa taagactcga tggctctccg
     4981 ccaacgacgc caacggcctg cgtgccaact ggtcgcctcc ctggtacatc tacaccgaac
     5041 attttgaaat tcagaccaac gtcacactcg cagaggcgat cagttttggc cgtcggctcg
     5101 aggcgtttca cgacgtcttc atggcgctcc tggtcgacat tctcggcgaa aatctcccgc
     5161 tggtccggcg cttcaaaagt ccatcgatga acggcgagcc ggagtacaag cttcactccg
     5221 tcttctattt tggatccaag gacgaatatg tcgactttct gacgccgcga tacgggccca
     5281 aagtcgctga ggaaagtctg gggtattacg acccgcccaa gtcgggggct aacaagcgcg
     5341 cgcctgccta ttttttccgt gaccccaacg gccagttgcc gatgacggcc accctctatc
     5401 acgaggtgtc gcaccaactg ctcttcgaga cggccggctc caacggctac accaagaacg
     5461 tgggcaacta ctgggtcttc gaggggctgg gcacctactt cgagacggtg gaaccccagc
     5521 cggacggctc gatcgaggtc ggcggtcgcg tcggccgccg gttggaggag gccatccgct
     5581 cgctggccct cctcggccgg accatcccgc tggagcagtt cgtcaactac gacgaggcgg
     5641 ccttctcgca cgacgattcc gccagat
//
LOCUS       DATAQL010001013         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1018890, whole genome shotgun sequence.
ACCESSION   DATAQL010001013 DATAQL010000000
VERSION     DATAQL010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018890"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..804
                     /locus_tag="VHS97_17565"
     CDS             166..804
                     /locus_tag="VHS97_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3450066.1"
                     /translation="MNDNQPINSGGQPRRPPWNKGKLVGPKPPLRPSHVWCIRTKLQI
                     AGRMRDLALFNLAIDSKLRGCDVVAVRVDDVAPNGYALDRATVRQKKTGRPVRFELTE
                     QTREALDDYLRLTGRKGGDFLFAGRAKTGRGLTTRQYARLVGEWISDIGLDPLKFGTH
                     SLRRTKVTLIYRRTGNLRAVQLLLGHTKIESTVRYLGIEIDDAIEIAEKIDV"
     gene            927..1514
                     /locus_tag="VHS97_17570"
     CDS             927..1514
                     /locus_tag="VHS97_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450067.1"
                     /translation="MMTTSGITKTVDEKEAVVRTLMSGPAYVHYGQIYVESGLEFPDM
                     TECFGGQRNGLCGAAVPGLLFLITGLHTGDVGFTVELYDKRPPVDEGWQEIVETSFRP
                     MGEAALVGWGCQWRQPLDLAEASYRVRYCAAGMDDGSKLDTRMAGEPEADRYLLLFWP
                     APLELDAVIKQTSGTAAYWHEFASQQQAPPAAATS"
     gene            complement(1683..2669)
                     /locus_tag="VHS97_17575"
     CDS             complement(1683..2669)
                     /locus_tag="VHS97_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450068.1"
                     /translation="MARHSLSFFADEQDAPVLLGRLNADPEIAFIVPDGPRMPPSPSP
                     PVEDRPAKGFIVQLASCDGDGYWQRWRTVRPLESRNDGEYILWHISAGPLVRDDGPGR
                     EFQPIPDPWAGWTSERPLCGPNILGTATIRLKLVTRHAAYTPEEQATIRPLVSYWINR
                     DLLVASDFQWTGASLQPDGSQQTARWAVGLKDWFSRNALGLRARDSIEVFWAFPSALQ
                     RLKAGMGYDARGFDLDESIGPPPATVKIIIQYYPAIEQQLRARRVGARFKQIPGVDVV
                     YKKSDGDVFEITVGDALKFSKKKLGRFPADEEVKALAEEVEALAASAPAVRR"
BASE COUNT          579 a          918 c          913 g          585 t
ORIGIN      
        1 tattctttgc cagctttgcc tggcgcactc agccatgggc cagtcgcgtc gctttaaacc
       61 gacatcaata gccaagtgct tgtcgatttc tcccgaaagc tcacgacaat cagcgtcgca
      121 gcaccagtgc aaggtggtca gtccctctaa tcaaggagac tgaccatgaa cgataatcag
      181 ccaattaact cgggagggca gccgcgtcgg cctccctgga acaagggtaa gttggtaggg
      241 ccgaagccgc cgctgcggcc cagtcacgtt tggtgcattc ggacaaagct tcaaatcgca
      301 gggcgcatgc gtgacctcgc actgttcaat cttgcaatcg acagcaagct gcgcggctgc
      361 gatgtagtgg cggttcgagt cgacgatgtt gcgccgaatg gctacgccct cgaccgagcc
      421 acggtccgcc aaaagaaaac aggtcggccg gtccgttttg agcttacgga gcaaacgcgt
      481 gaggcactcg acgactatct gcgcctgacc ggtaggaagg gcggagactt cctttttgcc
      541 gggcgagcga agaccggccg cggtctgaca acccgccagt atgctcgttt ggtaggcgag
      601 tggatctccg acatcggtct tgaccctttg aagttcggta cccactcgct caggcgaacc
      661 aaggtgaccc tgatctaccg ccgaaccggc aatctgcgtg ctgtccaact tttgctcgga
      721 cacacgaaga ttgagagcac cgtccggtac ctgggcattg agatcgacga tgcaattgaa
      781 attgccgaga aaattgatgt ctgaagtacc cgggcagggc tgccgtgctc tgcccgccgg
      841 ccaaccgtag gagagggcca ggaagagaca tcgagagatc cggcagcctc tattgccggg
      901 ttccctcgtc gcccgcgttg gcgacgatga tgacgaccag cgggatcacc aaaaccgtgg
      961 acgagaagga ggcggtagtg cggaccttga tgagcgggcc ggcatatgtg cattacgggc
     1021 agatctacgt tgagagcgga ttggagtttc ccgacatgac cgaatgcttc ggcggccagc
     1081 gcaacgggct gtgcggagcc gcggtgcccg gcttgctatt cttgatcacc ggcctgcaca
     1141 ccggcgacgt cggcttcacc gtggagcttt acgacaagcg gccgccggta gacgagggtt
     1201 ggcaggagat tgtcgagaca tcgttccgcc ctatgggcga agccgcgctg gtcggctggg
     1261 gctgccagtg gcgccagccg ctcgacctcg ccgaggccag ctaccgggtc cggtactgcg
     1321 ccgccggaat ggacgacggc agcaaactgg atactaggat ggcgggagag ccggaggccg
     1381 atcgctacct gctgctgttc tggccggccc cgctggagct ggacgcggtg atcaagcaga
     1441 ccagtgggac ggccgcctac tggcatgaat tcgcgagcca gcagcaggcg ccacccgcgg
     1501 cagccacctc atgacagcgg ttcctcgcgt tctcgtcctc gccattttgc ttcttccggt
     1561 cggctacgca tgggtttcat cactctatca gattactgcc cgctcccatc ccgggtttcg
     1621 ggtagcgata gttactcatt attggcaggc aactgtgtgc ttcgcggacg cggtttctct
     1681 cgtcacctcc tcactgccgg tgcggacgcg gctagcgctt cgacttcttc cgccagcgct
     1741 ttgacttcct cgtcggccgg aaagcggccg agcttcttct tggagaactt caacgcgtcg
     1801 cccacggtga tctcgaacac gtcgccgtcc gactttttat agacgacgtc gacgccggga
     1861 atctgcttga acctcgctcc cacgcggcgg gccctcaact gctgctcaat cgccgggtag
     1921 tattgaatga tgatcttcac ggtagcagga ggggggccaa tggattcgtc cagatcaaaa
     1981 ccgcgcgcat cataccccat gcccgcctta agccgctgaa gcgctgacgg gaaggcccag
     2041 aatacctcga tgctgtcgcg agcgcgaagc cccagagcgt ttcggctgaa ccagtccttg
     2101 aggccaactg cccatcttgc ggtctgctgg gagccgtcgg gttgcaggga ggccccggtc
     2161 cactggaaat ccgatgccac cagaaggtcc cgattgatcc aataggagac gagcgggcgg
     2221 atggtggctt gctcctccgg cgtatatgcc gcgtgtcgcg tcaccagctt cagtcggatc
     2281 gtcgcggttc ccaggatgtt cggtccgcaa agaggccgct ccgaggtcca cccggcccac
     2341 ggatccggaa ttggctggaa ctcgcgtcca ggtccgtcgt cgcggaccag cgggcctgcg
     2401 gaaatgtgcc agagaatgta ctctccatca ttccgactct ccaggggccg gaccgtccgc
     2461 catcgctgcc agtagccatc cccatcgcag ctcgccaact gtactataaa tcccttggct
     2521 ggtcgatctt ccacgggcgg cgatggcgac ggcggcatgc gcggaccgtc gggcacaatg
     2581 aaggcaatct ccggatcggc attcagccgg ccgagcagga cgggcgcatc ctgctcatca
     2641 gcaaaaaatg acagcgagtg acgagccatt tcctgagcct tcccctcctt cgcatgaagc
     2701 aaaggagccg agttgactgt tgcaaccgag attgcgacaa caattaccgt cgccgtagct
     2761 ctgcgctgct gcggtcgttg tctcaaggga tccccacgtg agcaaagtat ccagtcgtat
     2821 ttgtcattat agctggccgc gatacacgat aacgatggtc gcggtaggga cgacgatcgc
     2881 tctccgcccc ccgcacagat cccggcgtgc gctgctaacg caccgggctc ctccctcggg
     2941 ttctggcgtc gaagcggtga catggcaggg cgtgtagcgt cttgattggc ggaaa
//
LOCUS       DATAQL010001014         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2849843, whole genome shotgun sequence.
ACCESSION   DATAQL010001014 DATAQL010000000
VERSION     DATAQL010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2849843"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VHS97_17580"
     CDS             complement(<1..1820)
                     /locus_tag="VHS97_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEX3450069.1"
                     /translation="MNHNDRLRKAGFVASICLRLALIAIMTLTTLARARGDEPAPSPI
                     LHFTDGSFAAGELAESTRPGVFRWQASGFVSPFQFAVSRVNAIHWPPPKSVAKPGGDF
                     CFELAGGDILFGAVNALDGKQAELEIPRVGRLHIDRSSIHRIYRWQGSADLVYLGPNG
                     LTGWSETVPRRLGGNSDVNVWGPDGRPIRREPASPKMGWSEESGQLVTDREGASIRGN
                     FHIPAKSSIEFEISWKSKPDFVFALGVDDQDQGTVKRAFRFEAWGGDLVIQREVETEA
                     DLAVVQEISPGAGRAHLQAYLDQEAGRILVCSQSGKQLASLKVGGAGSKAFPGLYLSN
                     LRGDLRLEWLRISHWSGELPREVQVDQSRIHRADGSIVYGQVTGFDSSSREFLINNDS
                     RETRIAEKLISGVFLSLPTDEKPRTVRAVYQDGTRLSGELERLENGSLVLKVAGIFET
                     PRLPLTGLRSIVVMKQEVETPLAKSDSTGRLELEGLRLPGELVDGRMEAGSSCLVWQP
                     LGSEIASALKPGISARIIYKEPPPPPPVQPRTPVQNQRMIMVPAQPPQPPRAGGMVAR
                     FAQALAEKPPPTESPADERRALYLRDGDVIPSIITKIDEEG"
     gene            complement(1833..2945)
                     /locus_tag="VHS97_17585"
     CDS             complement(1833..2945)
                     /locus_tag="VHS97_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX3450070.1"
                     /translation="MHVLTLDMWESVGRRIDESRIFRRKCFTQAFFATALTSIVATPA
                     HAQLPAPRFGEVVPRDVREMYDRGLQYLATTQGEKGDWGNSGGQDGPGTTGMALMVFL
                     ACGEDPNFGPYSNHVRRSLRNIIEAQDSTTGIMGMSMYHHGFATLALAEAYGTVDERN
                     LWPDGKGPRSIGQALELAVRGAVTSQKKNPAGAWRYSPDARDADTSVSGAVLVGLLAA
                     RNAGIEVPDEAINKAISFYAQMTSASGEVAYAVGMGSFGESLARSSIATLVFSVARRK
                     DLKQFKATLDYLKQRIDQVPQQMWQEYASYYQAQALFQGDMAAWEKWNKLLVRQLKQA
                     QAPDGSFRGQLGGAVSTSLSLLALAVNFRFLPIYER"
     gene            complement(2959..>3870)
                     /locus_tag="VHS97_17590"
     CDS             complement(2959..>3870)
                     /locus_tag="VHS97_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450071.1"
                     /translation="MNARAITMTFNHIRIEWLIVAIMCAVGATGRLARAQDEDEPKPQ
                     VNAEPPRPVMVRDEDFDRWVFSLPGGKDAMRLRLETILTRRVARIAHQCALTEVQKKK
                     LLLAGRGDIKRFFDRVEEMRAKFDRDADDRAELVQFRDEIRSLRVVSSEDLFGKGSIF
                     SKVFKSTLTAHQSALYENVARDGAHSRHGTTIRWVVATLDTVLELSPVQHGQLEALLV
                     EQTRPPRNFGEYDYYGVLFQVSKLPENRFKSILSDDQWAKLSKHIDASRRLLPKLKAG
                     GFIPEDDVAAAPGSADHAASPQEKKRG"
BASE COUNT          714 a         1236 c         1132 g          788 t
ORIGIN      
        1 ccttcctcat cgatcttggt gatgatcgac gggatgacgt cgccatcgcg taggtagagc
       61 gcgcgacgtt catcggcggg cgattccgtc ggtggcggtt tctcggccag ggcctgggca
      121 aagcgggcca ccataccgcc ggcccgggga ggctgtggcg gttgggcagg taccattatc
      181 atcctctgat tttgcaccgg tgtgcgcggc tgaactggcg gcggcggtgg tggctcttta
      241 tagatgatcc gcgccgagat gccgggtttg agcgcgctcg cgatttcgct gccgagcggc
      301 tgccacacga gacagctcga cccggcttcc atgcggccat caaccaactc gccaggcagg
      361 cgcagtccct caagctccag tcgtcccgtc gagtcgctct tggcaagggg tgtctcgact
      421 tcttgcttca tcaccacgat cgagcgcaag ccggtcagcg gcagcctggg cgtctcaaag
      481 attccggcga ctttgagcac gagcgacccg ttctccaacc tctcgagctc gccggagagc
      541 ctcgtgccat cctggtaaac cgcgcggact gtccggggct tttcgtccgt cggcagcgaa
      601 agaaaaacac cggaaatcag tttctcggcg atccgagttt cgcgcgaatc gttgttgatc
      661 agaaactccc gcgacgacga gtcgaacccg gtcacctgcc cgtagacgat cgatccgtcg
      721 gcgcgatgaa tgcgcgactg gtcgacctgt acctcgcgcg ggagctcgcc gctccaatgg
      781 ctgattctca gccattccag ccgaaggtcg ccccgtaggt tcgacaggta gaggcccgga
      841 aaggccttgg agcccgcgcc accgaccttc aggctcgcca attgcttgcc gctctgcgag
      901 cataccaaga ttcgcccagc ttcctgatcc agatacgcct ggagatgagc gcggccggca
      961 ccgggactga tttcctggac gacggccaag tccgcctcgg tttcgacctc gcgctgaatg
     1021 accaggtcgc ctccccaggc ctcgaagcga aacgcgcgct tgaccgttcc ctggtcctgg
     1081 tcgtcgaccc ccagcgcaaa cacaaagtcg ggcttactct tccaggaaat ctcaaactcg
     1141 atgctcgatt tggccgggat gtggaaattc ccccggattg atgctccctc ccgatccgtc
     1201 acaagctggc ccgactcttc agaccaaccc attttcggcg aggccggctc gcggcggatt
     1261 ggccgaccgt cgggccccca cacgttcaca tcggagttgc caccgagtcg ccttggcacc
     1321 gtctcgctcc agccggtcag tccgtttggg cccaggtaca ccaggtcggc acttccctgc
     1381 cagcgataaa ttctatggat gctggagcga tcgatatgca gccggccgac cctggggatt
     1441 tctagctccg cttgcttgcc atcgagagcg ttcacggcgc caaacaaaat atcgccaccg
     1501 gcaagctcga agcagaagtc tccgccgggt ttggccaccg acttgggcgg aggccagtgg
     1561 atggcgttga cacggctgac cgcgaattga aacggtgaca caaagcccga tgcctgccag
     1621 cggaagactc cgggccgggt cgattccgca agctctcccg cggcgaagct gccgtcggtg
     1681 aagtgcaaga tcggtgacgg cgcaggttcg tcgccgcgcg cccgagccag ggtggtcaga
     1741 gtcatgatcg ctatcagcgc gagccgcaag caaattgagg cgacgaaccc tgcttttctc
     1801 agtcgatcgt tgtgattcat cgggttatcg acctatcgtt cgtagatcgg caagaaacga
     1861 aaattgaccg ccagagccag gagtgacagg gacgtgctga ccgctccacc cagctggccg
     1921 cggaagctgc cgtcgggtgc ttgtgcctgt ttcaattgcc ggaccaggag cttgttccat
     1981 ttttcccagg ccgccatgtc gccttgaaag agtgcctgag cctggtaata gctggcatat
     2041 tcctgccaca tttgctgcgg cacctggtcg atgcgctgtt tgagataatc gagggtcgcc
     2101 ttgaactgct tcaaatcctt gcggcgcgct acggagaaga cgagcgtcgc tatcgagctg
     2161 cgagccagtg actcgccaaa gctccccatg ccgaccgcat aggcgacttc gcccgaggcc
     2221 gaggtcatct gagcgtaaaa cgagatggcc ttgttgatgg cctcgtcggg cacctcgatc
     2281 ccggcgttcc gagctgccaa gagtcctacc aagaccgccc cgctgaccga ggtatccgca
     2341 tcgcgggcgt cgggagagta tcgccaggcg cctgctggat ttttcttctg ggatgtgacc
     2401 gcgccgcgca cggccagctc gagggcctgc cctatcgacc gcggaccctt gccgtccggc
     2461 cagagattgc gctcgtccac cgtgccgtac gcttcagcca gggcgagagt ggcaaagccg
     2521 tgatggtaca tgctcatccc catgatgccc gtcgtggaat cctgggcctc gatgatattg
     2581 cgaagcgagc ggcggacgtg attgctgtat ggcccgaagt tcgggtcttc accgcacgcc
     2641 aaaaagacca tgagcgccat gcccgtcgtg ccgggcccgt cttggccgcc gctattgccc
     2701 cagtcccctt tttcaccctg cgtggtcgcc aagtattgca ggccccggtc gtacatctcg
     2761 cgcacgtcgc gcgggaccac ctcgccaaac cgaggcgccg gcaattgggc atgcgcgggc
     2821 gtcgcgacaa tcgatgtgag ggccgtcgcg aagaacgcct gcgtaaagca ctttcgacga
     2881 aagatgcgag attcatcaat ccggcgaccg acgctttccc acatatccag tgttaagaca
     2941 tgcatcttga gattccttct aacctcgttt tttctcttgt ggcgaagcgg cgtggtcggc
     3001 cgaccccggc gcggccgcga cgtcatcttc agggatgaac ccacccgcct tcagcttcgg
     3061 caagagccgt ctcgacgcgt caatgtgctt gctcaacttg gcccattggt catcgctcaa
     3121 gatcgactta aacctgttct cggggagctt ggacacctgg aacaacacgc cgtaatagtc
     3181 gtattcaccg aaattccggg gtgggcgggt ttgctcgacc aacagggctt ctaattgacc
     3241 gtgctgtacg ggactcagct cgaggacggt gtcgagagtc gctacgaccc agcgaatcgt
     3301 cgtcccgtgg cgcgagtgcg ccccgtcgcg agccacgttc tcatacagag cggattgatg
     3361 ggcggtcagc gtcgacttga acactttaga aaagatcgat cccttgccaa acaaatcttc
     3421 gctcgagacg acccgcagcg accggatctc gtcgcggaat tgcaccaact ccgctcgatc
     3481 atccgcgtcg cggtcgaact ttgccctcat ttcctccacg cggtcgaaaa acctcttgat
     3541 atcgccccgg ccagccagca gcagtttttt cttttggacc tctgtgagtg cgcactggtg
     3601 ggcgattctg gcgacgcgtc tcgtcaagat cgtctcgagc cgcagacgca tcgcgtcctt
     3661 ccccccaggt agactgaaaa cccatcgatc aaagtcctca tcgcgcacca tgactgggcg
     3721 cggtggttca gcattcactt gcggtttcgg ctcgtcttcg tcttgcgctc gcgccaatct
     3781 tcccgttgcg ccgacggcgc acattatcgc cacgatcagc cactcgattc ggatatggtt
     3841 gaaagtcatg gtaattgctc gcgcgttcat
//
LOCUS       DATAQL010001015         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_254372, whole genome shotgun sequence.
ACCESSION   DATAQL010001015 DATAQL010000000
VERSION     DATAQL010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254372"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..2051)
                     /gene="ggt"
                     /locus_tag="VHS97_17595"
     CDS             complement(414..2051)
                     /gene="ggt"
                     /locus_tag="VHS97_17595"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3450072.1"
                     /translation="MSQEGHASDVGREVLRRGGNAIDAAIATAFALAVTLPEAGNLGG
                     GGFIVAYLADRREIVTVDFREMAPQSSSPGMYLDKDGKLKARHRAGAWAAGVPGTVRG
                     LGLAHARFGKTPWSELVLPAVRLARGGFPISADLASSLNRQLATANAGERNAPRGGDH
                     GRLGDFSESVAAFGKPDRTPWRAGDRLIQRDLAETLNRIAETGPDEFYTGRTAELIAN
                     YMERNNGFVSKSDLSSYQAKLRPPVHTSFRGFEVYTIGPSSAGGIVLCQMLNILERFD
                     LKKDGRDSPQSLHRITEAMRRAFYTRATMLADPEFLAIPIDELTSKAYAAKLAGSIGD
                     KATPSIALANFPIEPAESDHTTHLSTIDQAGNAVALTYTLEESYGAKCVVAGAGFLLN
                     NEMGDFNLIPGRTDTAGRIGTPANQIAPHKRMISSQTPTLLLKDGRARVITGSPGGRT
                     IPNTTLWIVLNLVEFGLGPKEAVLAPRTHHQWFPDVLTLEGRSWADPTRIALTAMGHK
                     LRTIEHQGNANTIVVDPDGRLHGIADPRRSTTKASGD"
     gene            2318..>3465
                     /locus_tag="VHS97_17600"
     CDS             2318..>3465
                     /locus_tag="VHS97_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3450073.1"
                     /translation="MIAKDRGRGLWLFSFVLAVVVIVAVYPSRAQQSTPSKFQVPPAA
                     PTTKADPRYKVNVKDIDVPGLKQTQIPVNPGDPIATVNGQIISRQQLADECVARKGKE
                     ILETLIHRTLIDQALRSRKLEVTAADIDQEIDTVAQRFGIGREGWLRTLDKERGISPV
                     QYARDIIYPALALRKLCSGRVTVTSDDMKNAFEAQYGEKLRCRMILVDKLPNARAIWE
                     QLRQNPGGFEKIAQEQSMDSGSRSLGGLLGEPITRHAYPQNLSDAAFQQLVDGDPADK
                     DPSHKPKDGDFTGPIQVSEMAWVILRRESVVPAVKDANLSDKRVREQTYEMIYEVKLK
                     EAMQGIFQELLKSAAIENQLAGTIKLANEEKDPNFGVDRDVKLMSNPAA"
BASE COUNT          737 a         1016 c         1012 g          700 t
ORIGIN      
        1 tcgatgcatg tgaaaagttc catcgctaat gcgcaacacg atcgttggac tatccgaatg
       61 ggaaccactc gccatcatgg tcaactccac tggaaaatgc gggattcgcc cgttctaaat
      121 gcccttgcac gatcctggga gggcgagcct cccggcgagc caccgcgcaa tccggctcgg
      181 acggagcctc gccctcccgg aatcacgcaa ggcggtttag agcaagccta gctcggattc
      241 gataggtatg gcgttcgcgg aaatcgtgcc tgacacatat ctctttaggg gtcaggtcac
      301 gagcgcaacg acgtcactcc ttattactac gccgagaaac gaagcgtgtg ccgcgccgcg
      361 attagtgcgg agtttcgaaa atcaacacgc gtggaacgag cgtcgtttgt gcctcaatcg
      421 cccgaggcct tcgtcgtcga ccgcctcgga tcggcgattc cgtgcaggcg gccatcggga
      481 tcgacgacga tcgtgttggc attcccttgg tgctcgatcg ttctcagctt gtgccccatg
      541 gcggtgagtg cgatgcgggt gggatcggcc cacgagcgac cttccagcgt cagcacgtca
      601 gggaaccact gatgatgagt ccggggtgcc aggacggcct cttttggtcc caacccaaac
      661 tcgaccagat tgagcacgat ccacaacgtc gtattcggga tagtgcgtcc gcccggcgag
      721 ccagtgatca cgcgagctcg accgtccttg agcaagagcg tcggcgtttg cgagctgatc
      781 atcctcttat ggggcgcaat ctggttagct ggggttccga tccggccggc ggtgtcggtt
      841 ctgcctggaa tcaggttgaa atctcccatt tcattgttga gcaagaatcc agcaccagcc
      901 acaacgcatt tggcgccata cgactcttcg agggtatacg tgagcgctac ggcgttgcct
      961 gcttggtcga tcgtggaaag gtgggtcgta tgatcgcttt cggccggctc aattggaaaa
     1021 ttcgccagtg caatactcgg agtcgctttg tcgccgatcg aaccagccag cttggcggcg
     1081 tatgctttcg acgtcagctc gtcgatcggg attgcgagga attccgggtc ggcgagcatc
     1141 gttgccctcg tgtaaaaagc ccgccgcatg gcctcggtga tgcggtgcaa ggactgcggc
     1201 gaatcacggc catccttctt caagtcgaac cgttccagaa tattgagcat ctggcagaga
     1261 actattcctc ccgctgacga cgggccgatg gtgtagacct cgaagccacg gaacgaagtg
     1321 tggactggcg gacgaagctt ggcctggtag gaactcaggt cgctcttgct gacgaatccg
     1381 ttgttccgct ccatgtaatt tgcgatcaat tcggccgtcc gaccggtgta aaattcgtcg
     1441 gggccggttt cggcaattcg gttgagagtt tcggccagat cgcgctgaat cagccgatca
     1501 ccggctcgcc acggcgtacg atcgggcttg ccgaatgcag cgaccgactc ggagaagtct
     1561 cccagccggc cgtggtcacc accccttggt gcattccgct ctccggcgtt ggcggttgcc
     1621 agttgccgat tcagtgaact ggcgaggtcg gcggagatcg gaaaacctcc ccgcgccagc
     1681 ctcaccgccg ggagtacaag ctcagaccag ggtgtctttc caaatcgggc gtgagcgagt
     1741 ccaagtccgc ggacagtccc cggtacgccg gccgcccacg ctccggcccg atgacgagct
     1801 ttgagcttgc cgtccttatc aaggtacata ccgggactgg aagactgtgg cgccatctct
     1861 cgaaaatcga cagtgacaat ttctcgccga tccgcgagat aggcgacaat gaatccgccg
     1921 ccgcccaggt ttccggcctc cggaagcgtg acagcaagag caaatgccgt cgcgatcgcc
     1981 gcgtcaatcg cgttgccccc ccgccgtaga acttcccggc cgacatccga cgcatgccct
     2041 tcttgtgaca ctgcaacatg tttcgagaag gcgtcatccc cgcgcgccac tcgaccaagc
     2101 ggaaaaacgg ccgcgagaat taataacgat gacgcccagc cgtttcgttg ttttcccatc
     2161 gttttgccca ttgctgtcgt gttcaaagtg ctgtggctcg actgcgcagg aattgagatt
     2221 gaccgtttcc tggcaaaaat ctagagtctc ggcccaactg cctcttgtct tgaggataat
     2281 tcgacaccaa ccaaaggaat tggggagacg ccacgtgatg atcgcaaagg atcgcggtcg
     2341 cggactgtgg ttgttcagct tcgtcctggc cgtcgtcgtg atcgtggcgg tttatccgtc
     2401 tcgagcacaa cagagcaccc cgtcgaagtt tcaggtgccg cctgcggcac ctaccaccaa
     2461 agccgatccg cggtacaagg tcaatgtcaa agacattgat gttcccgggc tgaagcaaac
     2521 acagatcccg gtcaatcccg gtgatccgat cgcgaccgtt aacggtcaga tcatctcgcg
     2581 ccagcagctc gccgacgaat gtgtggctcg taagggcaag gaaatcctcg aaacgctcat
     2641 ccaccgaacg ctgatcgacc aggctttgcg ctccaggaag ctggaggtca ctgcggcaga
     2701 tatcgatcag gaaatcgaca cagtcgccca gcgattcggg atcggccgcg aggggtggct
     2761 gcggaccctc gacaaggagc gcggcatcag ccccgtgcag tacgcccgag acatcatcta
     2821 cccggcgctc gccctgcgaa aattgtgctc cggcagagtg acggtcactt ccgacgatat
     2881 gaagaatgcg ttcgaggctc agtatggcga aaaactgcgg tgccgcatga ttttggtcga
     2941 caagctgccc aacgccagag cgatttggga acaactgcgg caaaacccag gaggctttga
     3001 aaagatcgcc caggaacagt cgatggactc gggaagtcga tcgctgggag gacttttggg
     3061 cgagccgatc acgcgtcacg cctatcctca gaacctctct gacgcagcat ttcaacagct
     3121 cgtcgacggc gatcccgcgg ataaagatcc ttcgcacaag cccaaggatg gcgacttcac
     3181 gggtccgata caggtcagtg aaatggcctg ggtgatcctg cgcagggagt cggtcgttcc
     3241 cgcggtgaaa gacgccaacc tgtccgataa gcgagttcgg gagcagacct atgaaatgat
     3301 ctacgaggtc aagctcaagg aggccatgca aggtattttc caggaattgc tcaagtcggc
     3361 tgccatcgaa aaccagttag cgggtaccat caagctggct aacgaggaaa aagatcccaa
     3421 tttcggtgtc gaccgcgatg tcaagttgat gtcgaaccca gccgc
//
LOCUS       DATAQL010001016         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4224531, whole genome shotgun sequence.
ACCESSION   DATAQL010001016 DATAQL010000000
VERSION     DATAQL010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4224531"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1683
                     /locus_tag="VHS97_17605"
     CDS             <1..>1683
                     /locus_tag="VHS97_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX3450074.1"
                     /translation="STLLHALITNGALRYSPDQLELYLIDFKKGVEFKVYAAMNLPHA
                     RVIAVESEREFGLSVLQRLDVELKERGEIYRQYGCQDLAGYRECGRDVPMPRVLLVID
                     EFQEFFVEDDKIAQEVTLLLDRLVRQGRAFGMHVILGSQTLGGQYSLPKATLGQMAVR
                     IALQCSEADAHLILSEDNTAARLLTRPGEAIYNDANGMMEGNNLFQVVWLPDANREKY
                     LERIQEMVTEHRIQKPPPIVFEGNLPADVSKNPLLNNMLQATEWPEPPRADYAWLGDA
                     IAIKDPTSVVFRPQSGSNVLVVGQNDEAALSMMIMSVLSIAAQHPPAGPRTSRFYLLD
                     GSQVDSALAGQLGRLKDIVPHPVTNVSWRDLAATYGELAAEVKRRQESTSDDQVPMYI
                     FIFDIARFRDLRKADDDFGYSRYDEDKPAPPSKLFTDVLRDGPPVGIHTVVWCDSVNN
                     MNRTFDRQGIKEFENRILFQMSANDSSTLIDTPAASKLGENRALYFSEEENKIEKFRP
                     YGLPEIEWLDGVKRRFAARPRPEVHVQPDGEANRAAPLEVNGQSDGEADRAT"
BASE COUNT          358 a          493 c          505 g          327 t
ORIGIN      
        1 aatcgaccct gttgcacgcg ctcatcacga atggcgcgct gcgatacagc cccgaccagc
       61 tcgagcttta tttgatcgac ttcaagaaag gtgttgagtt caaggtctac gccgcgatga
      121 acttgccgca tgcccgggtc atcgccgtcg agagcgaacg cgagttcggc ttgagcgtgc
      181 tccagcgatt ggacgtcgag ctcaaagagc gaggcgagat ttatcgccag tacggctgcc
      241 aagacctggc cggctatcgc gaatgcggcc gcgacgtacc gatgccgcgc gtcctcttgg
      301 tcattgacga gttccaggag ttcttcgtcg aagacgacaa gatcgcgcaa gaggtgacgt
      361 tgcttctcga tcgcctggtg cgtcagggtc gtgcgtttgg catgcacgtc attctgggct
      421 cgcaaacgtt gggcggccag tattcgctac ccaaggccac cctgggtcag atggctgtgc
      481 gaatcgcgtt gcagtgcagt gaagccgatg cgcacctgat cttgagtgag gacaacacgg
      541 ccgcgcgatt gttgacccgc cccggcgagg cgatctacaa cgacgccaac ggcatgatgg
      601 agggcaacaa tctcttccag gtggtttggc tgcccgatgc gaatcgggaa aagtacctgg
      661 aacggatcca ggaaatggtc actgaacacc gtatccaaaa gcctcctccc atcgtgttcg
      721 agggaaacct gccggcggac gtgagcaaga atccgctgct gaataatatg ctccaagcga
      781 cggaatggcc cgaacccccg cgggccgact acgcgtggct gggcgacgcg attgccatca
      841 aagaccctac gtcggtggtg tttcgtcccc agagcggcag caatgtgttg gtcgtcggcc
      901 agaatgacga ggcggcattg tcgatgatga tcatgtccgt actgagcatc gccgcgcagc
      961 atccgccggc cggtccccgg acgtcccgct tttacctttt ggatggcagt caggtcgatt
     1021 ccgcactggc cggacaactg ggccggctca aagacatcgt gccgcatccg gttaccaatg
     1081 tctcttggcg cgacctggcc gcgacttatg gcgaactggc cgcggaagtc aagcgtcggc
     1141 aggaatctac gtccgacgat caagtgccaa tgtatatctt tatcttcgac atcgcgcggt
     1201 tccgagacct gcgcaaggcc gacgatgatt tcggctactc gcggtacgat gaagacaaac
     1261 ccgcgcctcc ttccaagctt ttcaccgacg tgctgcgcga cgggccaccc gtcgggatcc
     1321 ataccgtggt ctggtgtgat agcgtcaaca acatgaaccg gactttcgat cgtcaaggaa
     1381 taaaagagtt cgaaaaccgg atcctgtttc agatgagcgc caacgattcg agcacactca
     1441 tcgacacgcc ggcggccagc aagttgggtg aaaaccgggc cctgtatttc agcgaggaag
     1501 aaaacaagat cgagaagttt cggccctatg gacttccgga aatcgaatgg cttgacggcg
     1561 tcaagcggcg gtttgccgct cgtccccgcc ccgaagtcca tgttcagcca gatggggaag
     1621 ccaaccgcgc ggccccgctg gaggttaacg gtcagtcgga tggcgaagcc gatcgcgcga
     1681 ccc
//
LOCUS       DATAQL010001017         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1934483, whole genome shotgun sequence.
ACCESSION   DATAQL010001017 DATAQL010000000
VERSION     DATAQL010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934483"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VHS97_17610"
     CDS             complement(<1..1466)
                     /locus_tag="VHS97_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HEX3450075.1"
                     /translation="MNSSYVEPSRVSTLDLAPSPVLLIDPRGEDPIRARILGLERLES
                     HARLMARACTLAPRRRANSPLLERFVDNKRVLSWVHDQLSTRGDLHGIDAEWLVDNFH
                     IIEDSLREVARDLPPGYDAQLPKISAPPLVGYPRVYALALALVAHTDSELDETRIVRF
                     VSAFQESAPLTIGELWALPTMLRLVLVDNLRRLSEKMIWRWEERRRAEQWAKSALADT
                     QGPYDEAKNKGPSQAPPPLAELSDPFVVRLLQLLRDQGPVNAAVEHLEAELSAHGSEP
                     NEVLKREQSRQAADQVTVGNCVLSLRLLSAIDWNAFFEKSSPVEAILRDDPAGIYPRQ
                     EFATSDRYRRVVEIIARQSDADEMEVSRKAVELAVQGRGAGEPRDHVGFYLIDRGQKE
                     LKSAFRFRPTWRERLLDLVLGRPQSVYFGSIVIGLAALLALVVIFALGGGLTNFWWIL
                     AIAAAALLPISELVVGLVNQLFTLFVPPRVLPKLDVKHG"
     gene            1716..2138
                     /locus_tag="VHS97_17615"
     CDS             1716..2138
                     /locus_tag="VHS97_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450076.1"
                     /translation="MKIYRVSRLYRWSWIGAKLVLTIVAGGLYLAAVTHPTPLAVRLM
                     LLAGLALFGWLFYVRLPKIPTEITVSDDGWVSFRSSRGTTRVQAADIRSIGRSFGRRT
                     LRVVHAGGQVQVPNRFRKLVDLLLTVKGLNPSLEIRGF"
     gene            3140..3796
                     /locus_tag="VHS97_17620"
     CDS             3140..3796
                     /locus_tag="VHS97_17620"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX3450077.1"
                     /translation="MGTNPLRFLKAQPRFFTAVLVGTLVWLFLAQGSRPSTRLLLAWD
                     CATGLYLMMALVMMARSNIDRIRYRAALQDEGQLVILGLTIITALISLGGIVAEMATA
                     KTLKGRDEWQHIALSGLTVLLSWTFTHTMFAIHYAHEYYAGSENELAEGLDFPGKEPP
                     DYWDFVYYSFIIGTACATADVNVTSKTMRRLTTLHCVVAFFFNTTVLALTVNISAGFF
                     "
     gene            complement(3951..>4236)
                     /locus_tag="VHS97_17625"
     CDS             complement(3951..>4236)
                     /locus_tag="VHS97_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450078.1"
                     /translation="WISRWGLVVEQAGFTTISPGKSVLQTTQLRAKTLERALAMRMLM
                     EGGLDPGSALVMIGAERQPAAKSSLRYHLRTRRALTHGSRVRARAGRRNS"
BASE COUNT          829 a         1279 c         1287 g          841 t
ORIGIN      
        1 ccatgcttca cgtcaagctt gggcaaaacg cgcggcggca cgaacagggt aaatagctgg
       61 ttgaccaaac ccaccaccag ctcggagatg ggcaacaacg ccgccgccgc gatcgcgagg
      121 atccaccaaa aattggtgag gccccccccg agcgcgaaaa tgacgaccag ggccaacagt
      181 gcggcaagcc cgatgacgat cgaaccgaag tagaccgatt gcggccgccc cagcacgagg
      241 tcgagcaagc gctcgcgcca ggtggggcga aagcggaatg cgctcttgag ctctttctgg
      301 ccgcggtcga tcaaatagaa gccgacgtgg tcacgcggct cgcccgcgcc acggccctgc
      361 accgccagct cgaccgcttt ccgtgacact tccatctcgt ccgcgtccga ttgtcgggcg
      421 atgatctcga ctacccggcg ataccggtcg ctggtggcaa actcctggcg cgggtaaatg
      481 cctgcgggat catctcgcag gatcgcttcg acgggactgc ttttctcgaa gaatgcattc
      541 cagtcgatgg ccgagagtag gcgcaggctg agcacgcaat tgcctaccgt cacctggtcg
      601 gcggcctggc ggctttgctc gcgcttcagc acttcgttcg gttcagagcc gtgggcggac
      661 aactcggcct cgagatgctc gacggccgcg ttcacggggc cttggtcgcg caagagttgc
      721 aacagtcgca cgacaaacgg atcgctcaat tcggccaggg ggggaggcgc ctggcttggt
      781 cccttgttct tggcctcatc ataaggtcct tgcgtatccg ccagcgcgct cttggcccac
      841 tgttccgcgc ggcgccgttc ttcccacctc cagatcatct tctccgacaa tcgccgcaag
      901 ttatcgacaa gtaccagacg aagcatggtg ggcaacgccc agagctcgcc gatcgttaat
      961 ggggcggatt cttgaaacgc gctgacaaat cgcacgatgc gtgtctcgtc cagctcgctg
     1021 tcggtgtggg cgaccagagc cagcgccagg gcgtaaacgc gtggatagcc cacgagcggc
     1081 ggagccgaga tcttgggaag ctgagcatcg tagcctggcg gcaggtcgcg agcgacttcg
     1141 cggagcgaat cctcaatgat gtgaaagtta tcgaccagcc actccgcgtc aattccgtgc
     1201 aggtcgccgc gcgtggagag ttgatcgtga acccatgaga ggacacgttt gttgtcgaca
     1261 aatcgttcca gtaagggact gttggcgcgc cgtcgtggcg ccagcgtgca tgctcgcgcc
     1321 atcagtcgtg catggctctc cagccgctcc aggcccaaga tccgcgcgcg gatcgggtct
     1381 tccccgcgcg gatcgatcaa gaggacgggc gaaggcgcca ggtcgagagt cgacacacgg
     1441 gacggttcga cgtaggacga attcatgctg tggacactct agtacgaact ctgatgccaa
     1501 cacggcaata agcttaagag atcgccgctg cgattaaaac gtgagttgcg gcctggggct
     1561 tgcggattcg cccatgcctc ggatctgacg gaagcaacaa cagtcgtttt tcctggctcg
     1621 attggtcgcg gactcgccct caataaatgg gccggctgac cgaataagaa ctgagggtgt
     1681 tagaagagct atacgccaac tacggggacg atcctatgaa gatataccgc gtatcacgcc
     1741 tctatcgttg gtcgtggatc ggcgccaagc tggttttgac gatcgtggcg ggaggtctct
     1801 accttgcggc ggtgacccac ccgacgcccc tggctgtccg gttgatgctc ttggcggggc
     1861 tggcgctctt tggctggctc ttttacgttc ggctccccaa aattccgacg gaaattactg
     1921 tgtccgacga cggctgggtt agttttcgaa gtagccgggg aacgacgcga gtccaggccg
     1981 ccgacatccg ctcaattggc cgcagcttcg gcagacgaac actgcgcgtg gtgcacgccg
     2041 gcggtcaggt ccaggtaccc aaccgcttca gaaaactggt cgaccttctc ctgactgtga
     2101 agggcctgaa cccgtccctg gagattcgtg gcttctgatc cgattgcaag gcccacacac
     2161 gatgcgtcaa tggaatctgg cataactgct agcagtttgc catgacacga tggtgagtgt
     2221 gttccacctg ggttcgtcat gctttccgca tccacggcgg gttcgctcgt ctcatttggc
     2281 gttgcgctcg attcgagcgt gtcgcgggca gcggacaacg aagcaggcaa atcggacgac
     2341 gcacattcca ccagttgcgg ggcgcggcgg agcttgagca gtgagtcgag cgagcgggca
     2401 aggccccggc cgcaagccag gtcgaagcga cgcaggcgct ctgcttccgg gctgtcgtca
     2461 aatgccagac ggtcggcggc caaggagtcg ttgatctcgg cccgaacgca atgcgcgtcg
     2521 gctttgcgtg cgacctctgc cgtcgcgccg ccacgagtat tcgacggcgt catcaacgat
     2581 ccactgttcg gcggctccgc ggggtaacag tctggccgtc caggccgttt tccggtcctc
     2641 gagcgccggc gggtcttcgt ccagcaacac cagcgtcgcg gccctcatgc cgtgcttgac
     2701 ggcgttgagc ttggagcgct gctaaatcgc agcaagtcgc gcgggtggat gctcacccag
     2761 gaacgttcga tgctggcgta ttcacggcgg tacagccagg cccggcaacg gacggccacc
     2821 acggcaacga gcaccatgag ccgcgccacg ctgacatgga agggcccgag accacaactt
     2881 cacgatttgg ccaccatgga ccccccttca cggaaatcaa gggccgttcc tgctcttgtt
     2941 gcagcgacga gagccgttcc actacgattg tcactgatta tcctcagtcg aacgggctgt
     3001 caagttagca accccaccga gccatttggc aaggaactga gcggcgcgcg agcgagcatc
     3061 ccctgcccaa atcgaatgac cgcgctgtcg ctgttaaccg gcaaacgtcg gcgagcacaa
     3121 ttcaaaggcg aaagctcgaa tgggaacgaa tcctctgcgg tttctcaagg cacaaccgag
     3181 gtttttcact gccgttctgg tcggtactct ggtttggttg ttcttggcgc agggctcgag
     3241 gccatcgacg cggttgctgc tcgcgtggga ttgcgctaca gggctatacc tgatgatggc
     3301 gctggtgatg atggcgcgat cgaacatcga caggatccgt taccgcgccg ccttgcagga
     3361 cgaggggcag ctagtgatcc tcggactgac gatcatcacg gcactcatca gcctgggtgg
     3421 catcgtcgct gaaatggcca cagccaaaac gctgaaaggt cgcgacgaat ggcagcatat
     3481 tgcgctttcg ggccttaccg tgttactgtc gtggaccttc acgcacacaa tgttcgcgat
     3541 ccattatgca catgagtatt acgcaggatc ggagaacgag ctggcggaag gactggattt
     3601 tccggggaag gagccgcccg attactggga ctttgtctat tattccttta tcattggcac
     3661 cgcctgcgcg accgccgatg tcaacgtcac ctccaaaaca atgcgcaggc taacgacgct
     3721 gcattgcgtg gtcgctttct tctttaacac aaccgttctc gcgttgacgg tcaatatcag
     3781 tgctggcttc ttctgactgg ggaacgcagg tccgccagtt tcttgacatt gcgaagtgat
     3841 ccgtgatgat cgcgatgcga tccgatctgc ctgggcgctg ggaatcggaa tcatcttcat
     3901 cgccaatgcg ttgcgtactt tttccggtac ttgaggaaat cgggacgcga ctagctgttg
     3961 cgccggcctg cacgcgcacg gaccctggat ccatgcgtca aagctcgtcg tgtccgaagg
     4021 tgatagcgta gactcgactt cgccgccggc tgccgttccg caccgatcat caccagggcg
     4081 gacccagggt caagaccccc ttccatcagc atgcgcattg ctagggcgcg ctccaacgtc
     4141 ttggcgcgaa gctgggtcgt ctgcagaaca ctcttgccgg gactgatggt ggtgaaaccc
     4201 gcctgctcga caaccagccc ccagcgtgaa atccag
//
LOCUS       DATAQL010001018         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5445628, whole genome shotgun sequence.
ACCESSION   DATAQL010001018 DATAQL010000000
VERSION     DATAQL010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5445628"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VHS97_17630"
     CDS             <1..439
                     /locus_tag="VHS97_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450079.1"
                     /translation="WDIVGGTSLGTPAWAAIVALADQGRSLAGKGSLDGGTQTLPALY
                     ALPSSAFNTIATSGTRGRVSAAATANTYTGRGTPVGYAVVAGLVADNTAIPLTTSTSV
                     QRAVKRNVSARGRKLTALPIRETTGFFHSSGSRRAVAKVETRH"
     gene            667..>1555
                     /locus_tag="VHS97_17635"
     CDS             667..>1555
                     /locus_tag="VHS97_17635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX3450080.1"
                     /translation="MRNRRRGLRPHLDILDDRCLLSGYASSAQTGYTPAQIASAYGLN
                     AITLSSSTGSTVKGDGSGETIALIEMYHDPNLTSDLKTFDAKYGLPNPSLTAVDQAGK
                     QTDSGWALEESMDVEWAHAIAPGANILVVEAAPSDSETQGLTSLLNAVNTARNTAGVA
                     VVSMSWGFDELPKEASYDSYFTTPAGHSGITFVASSGDNGSIEYPSASPNVLSVGGTT
                     LSIGISGSYGSETVWTSGGGGYSQYESEPSYQNSVQSTGLRSTPDVAFDGDPSTGAQV
                     YSTPPGSTKGSWQVVGGTSL"
BASE COUNT          315 a          489 c          471 g          280 t
ORIGIN      
        1 gtgggacatc gtgggcggga cgagcctggg cacgccggcg tgggcggcga tcgtcgcgtt
       61 ggccgatcag ggccgcagcc tcgcgggcaa gggaagcctg gacgggggaa cccagacact
      121 gccggctctc tacgcccttc cctcgagcgc attcaacacc atcgctacat ccggcacccg
      181 agggcgcgtg tcggcggccg caacggccaa cacgtacacc gggcgtggca cacccgtcgg
      241 ctacgccgtg gtcgcaggct tggtcgccga caataccgcg atcccgctca ccacaagcac
      301 ttccgtgcaa cgcgcggtca aacgaaacgt cagcgcacgg ggtcgcaagc tgaccgccct
      361 gccgattcgc gaaaccaccg gctttttcca ttcctctggt tccaggcggg cggtcgcaaa
      421 ggtcgagacg cgacactgac aatctctgta gcccccgcgt gtgaacagca ttgtataatt
      481 cgaggcgtta gccatcgtcg ctctgacaac cgatgcccaa tcggtgcgtg ggcagggccg
      541 tctccaattc tgtgtggtcg atcgtgcgac ttcgttccaa tcgtcatagt cgcttcaaat
      601 gtaccgtcgg cctgatttca tcattgacct tgcaatggat tgcgcatcaa gaggacatgg
      661 tcactcatgc ggaaccgccg acgcggcctc cgaccgcatc tggatatcct tgacgatcgc
      721 tgcctgttgt caggctatgc ctcatctgca caaacgggct acacgccggc ccagatcgct
      781 agtgcttatg gcttgaatgc gatcaccctc tcgtcctcga ccggctccac ggtgaaagga
      841 gacggcagcg gcgagacaat cgccctgatc gagatgtacc acgacccaaa cctcacctcg
      901 gacttgaaga cgttcgacgc gaagtatggc ctgcccaatc cgagcctcac cgcggtggac
      961 caagccggaa aacagactga cagcggctgg gctcttgaag agtcgatgga cgtcgaatgg
     1021 gcacatgcaa tcgctcccgg cgccaacatc ctggtggtgg aggctgctcc atcagattca
     1081 gagacccagg ggctgacaag cctattgaac gccgtaaaca ccgcgcggaa tacggcggga
     1141 gtggctgtgg tctcgatgag ctggggattc gacgagttgc ccaaagaggc ttcatacgat
     1201 tcctacttca cgacaccggc ggggcactcg ggaatcacgt tcgttgcgtc gagcggagac
     1261 aacggatcca tcgagtatcc ttccgcctca ccgaatgtcc tgtccgtcgg tgggaccact
     1321 ctgagcatcg gcatctcggg gagctacggc tcggagacgg tctggacctc agggggcggg
     1381 ggctatagtc agtacgagtc ggagccgagc tatcaaaatt cggtccaatc tacgggacta
     1441 agaagcactc ccgacgtggc cttcgatggc gatccgagca ccggcgccca agtctactcg
     1501 acaccccctg gctcgaccaa gggaagctgg caggtggtcg gcgggacgag cctgg
//
LOCUS       DATAQL010001019         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1019424, whole genome shotgun sequence.
ACCESSION   DATAQL010001019 DATAQL010000000
VERSION     DATAQL010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019424"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1875
                     /locus_tag="VHS97_17640"
     CDS             382..1875
                     /locus_tag="VHS97_17640"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3450081.1"
                     /translation="MPIDVLPPEVAALLAALPPDQELPLKVLAPFVLQRVPLANACLT
                     AWSYMLNSDFLDDLYDRHRGRSYEDILTFPNFVELARDALVLYKGSGRASLERADEQE
                     QLPTCHGAFYGKLARIPIGLSLGFMEECTERLRALRPTGVWAEPLPASLAGMTVTIVD
                     GKQIKNVAKRLLPLRTKPGSIIGGKILGIYVPAEGLVVAMSADPDGEANDVRLMPEAI
                     RRANARIKGVKLWVADRQFCDLNLPTVLTDQGDHFLFRRTMKMGFAADPSRPQKQAVD
                     SQGRTVIEQWGWIGAEKEKRRRYVRQIHLVRPGQDDVYLITDLLDGKAYPADDLLLVY
                     LKRWEIERVFQKITEVFSLQQLIGCSPEATIFQASFCMVLYNLLQLMCAFMAASQKDL
                     VAEAVSIEKLFEDVQRQLTTLVVLFPSATIAGWYTAVWTPEEVSRRLQVLMGASWTPR
                     YRKAVNKKPRPKVTKAKSSGAHTSVHKVLHAERKTQQPARGDPKAKS"
BASE COUNT          437 a          706 c          687 g          418 t
ORIGIN      
        1 ccgtgatatc cgacgaccgt tcgatggtaa tccgagaatc gcggcatcgc tcaggtcgaa
       61 ctcctgccag gtatcctaag attctaacgg aaaatggcag ggagctgcaa tttgggcgtg
      121 cgacgacacc agggccgttt cattcttcgt gggagggcga ggccactact gttcacgact
      181 ttgattggtg taagatggcc gtgcggcccg acatgacgtc ccactcattc ccgacacgga
      241 tgtcgcacca tgatcgttcc tcccaaggct ggcgcacgta aagagccacg gacgagccga
      301 cgcattggag ccaatcccct ttcgaagcct cggcctgggg tcaagccgcc aatccctgag
      361 ccaccaccgc gagataccca gttgcccatc gacgtcctgc ctcctgaggt cgccgccctc
      421 ctggcggctt tgcctccaga ccaggagctg ccgctgaaag ttcttgcccc gtttgttctt
      481 cagcgggttc cgttggccaa cgcctgtctg acggcgtggt cgtacatgct gaactccgac
      541 tttctcgacg acctctatga tcgtcaccgc ggtcgctctt acgaggacat cctcacgttc
      601 ccgaacttcg tcgaactggc tcgcgatgcc ctcgtcctct acaagggcag cggccgggcg
      661 agcttggagc gcgcggacga gcaagagcaa ctgccaacct gtcatggcgc cttctacggc
      721 aagctggccc gcatcccgat cgggctgagc ttgggcttca tggaagaatg caccgagcgg
      781 cttcgcgcgc tccgcccgac gggcgtctgg gccgagccct tgcccgcatc gctggccggc
      841 atgaccgtca cgattgtcga cggcaaacag atcaagaacg tcgcgaagcg cttgctccca
      901 ctgcgcacga agccgggcag catcatcggc gggaagattc tgggaatcta cgtcccggcc
      961 gaaggacttg tcgtggcgat gtccgcagat cccgatgggg aagccaacga cgtccggctg
     1021 atgccggagg cgatccgacg agccaatgca cggatcaaag gcgtgaagct gtgggtggcc
     1081 gatcgccagt tctgcgacct caacctgccg accgttctga ccgaccaggg agaccatttc
     1141 ctgtttcgcc gcacgatgaa gatgggattt gcggcggacc caagccgtcc ccagaagcag
     1201 gcggtggact cgcagggccg taccgtgatc gagcagtggg gctggatcgg cgcggagaag
     1261 gagaaacgac gacggtacgt gcggcagatt catttggtcc gtcccggcca ggacgatgtt
     1321 tacctgatta cggatctgct cgatgggaag gcatatccgg ccgatgacct gctgttggtc
     1381 tacctgaagc gatgggagat cgagcgggtg tttcagaaga tcaccgaggt cttcagcctg
     1441 cagcagctta tcggctgttc gccggaggcg acgatcttcc aagcctcctt ctgcatggtg
     1501 ctgtacaatc tgcttcagtt gatgtgcgcc ttcatggcag ccagccagaa ggatctggtt
     1561 gcggaagcgg tctcgatcga gaagctcttc gaggacgtgc aacggcagtt gacgacattg
     1621 gtcgtcttgt ttccgtccgc gacgatcgcc ggctggtaca ccgctgtgtg gactcctgag
     1681 gaggtcagtc gccggcttca agtcctcatg ggcgcgtcgt ggacgccccg gtaccgcaag
     1741 gcggtcaaca agaagcctcg gccgaaagtc acaaaggcta aatcctctgg cgctcatacc
     1801 tcagtccaca aggtgttgca cgccgaacgg aaaacgcaac agccggcgcg cggtgacccg
     1861 aaggcgaagt cgtgaatagc agtggcctcg ccctcccgaa gaccgctccg tgggagggca
     1921 aggctcctgc cgagccggcg gtggccctgg agaccgcaaa acacttcgcg atagcatcat
     1981 cctcgaatca cgcaaggtca tttagccgcc cctacggggc taacggaccc cactccggcc
     2041 gtctcagcgg gcccattgcc gaccccgttc cctctcaacg gagcccctcg acaacccgta
     2101 cgcggggcgg gtctgcgtag tcgttccaga gtcgctcgat gagcctctca tccggagggc
     2161 cgtcgtgcac gatcagccgg tagcgcgagg tgtagggacg gcccggcgca atctcgaagt
     2221 caccgagcac catcggcgca aagcaaaa
//
LOCUS       DATAQL010001020         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2395018, whole genome shotgun sequence.
ACCESSION   DATAQL010001020 DATAQL010000000
VERSION     DATAQL010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395018"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VHS97_17645"
     CDS             complement(<1..1028)
                     /locus_tag="VHS97_17645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX3450082.1"
                     /translation="MPKIGPAQILLVLLTFFLLAVLASMFMAWGWLIWRLLTRQPILP
                     EKPMVARQEPTWGLWSVLLIVLLYLVVGFTLSQGYALATGRSPARQTPPPAAKNDRAA
                     DGAEKHVDDKAIEPTFAMTEMMFVNAVAQVIMLVLAPLVLRCTSGAHLHDLGLSLSGW
                     WRQAAVGIVAMLITTPVVFLVQFGAISVWRPNSHPLEKMLREHFSVDVGYLALVTGVI
                     LAPMLEEMTFRAIFQGWLIRWMRRREYSTEPREILHELQSHEIEGDSVGQPAAYWEAA
                     ERSAVLLADPVPEKVPESRRAVWKAIVVTSLFFAAVHAAQWPAPIPIFVLSLVLGTIY
                     YRTGSLIAS"
     gene            1233..2456
                     /gene="tyrS"
                     /locus_tag="VHS97_17650"
     CDS             1233..2456
                     /gene="tyrS"
                     /locus_tag="VHS97_17650"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX3450083.1"
                     /translation="MRDVAHQLSILKRGVDQIVPEVEFLKKLDRSVRENRPLRVKYGI
                     DPTGIDVHLGHTVPLRKLRQFQELGHTAVIIIGNYTALVGDPSGRDETRAKLTQEQVE
                     ANAIDYLTQVGRIIDLSKAEVHKNGDWFSRWTFLEVLDLMRKMTIGQMTAREDFAKRI
                     AALKPVYLHECLYPLMQGQDSVEIKADVELGGTEQLFSLLVARDLQQSQGQEPQVAMT
                     MPILVGTDGTRRMGKSLGNYIGVAEHAENQFGKIMSIPDEPMRQYFTLLTELPITDVD
                     RILGPENNPRDAKEILGKEIVEQYHGPEAAERAAAEFRRRAAGEDPDQIPDVFLAADK
                     LDSEGRISTPVLLKELGLESSTSNARRLIEQGGVSVGGGDRRETITDVRALIGVSDGL
                     IVRIGKRKIARVRLV"
     gene            2541..3608
                     /locus_tag="VHS97_17655"
     CDS             2541..3608
                     /locus_tag="VHS97_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450084.1"
                     /translation="MAEHGLGLIGCGMIAEFHTRAINEIANARVVGAWSRNAANAAKI
                     AGLAKGGCATFDDLDALLAQPGLDIVCICTPSGAHMESAVRAARAGKHVVVEKPLEIT
                     LPRCDSIIEACDAAGVRLCTIFPSRFTAANIRLKEAIDLGRFGRLTLGDTYVKWWRTQ
                     EYYDSGGWRGTWQLDGGGALMNQAIHNVDLLTWLMGDVATITALTATLAHQRIEVEDT
                     AVAALRFKNGALGVIEAATSAYPGLLKRTEIHGDRGSARVEQDDLTLWEFQEKVPSDN
                     EVYAAMAGQTGFKAGASDPRGITHIGHRDQLIDFLEAIDTGRAPQVDGREGRKSVEII
                     RAIYRSAQTHTSVELPLDDEG"
     gene            3613..>4433
                     /locus_tag="VHS97_17660"
     CDS             3613..>4433
                     /locus_tag="VHS97_17660"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3450085.1"
                     /translation="MTELELAPTRSLIIIALIACALSAPARGSDKNPPAPLGADKDRV
                     GDEVRRIPWRGSRIKGSPDPPAPYTAELAFPHLKFNNPVVLVPAAGTNQLFLGEVKGR
                     IYSFPEDPGCTKADLVLDLAPLYPDFTAFYGLVFHPEFDKNRYVYLCYVLKNDLVDGS
                     VVSRFTVSRSDPPVIDPKSEQILLKFWSGGHNGACLVFGNDGCLYISTGDGASPTPPD
                     PMMTGQDCSDFLSSVLRIDVDHAEPGKAYRIPADNPFLNTPGVRPEIWAYGFRNP"
BASE COUNT          947 a         1371 c         1307 g          808 t
ORIGIN      
        1 gatgctatta agctgccggt gcggtaataa atagtaccga gcaccaacga aagcacaaaa
       61 atcgggatag gtgcaggcca ttgggcagcg tggacggccg cgaaaaaaag cgaggtcacg
      121 acaatcgcct tccagacggc acggcgagat tccggcacct tctccgggac tgggtccgcc
      181 aggagaacgg ccgagcgctc tgcggcctcc caatacgccg cgggttgacc gacagagtcg
      241 ccttcaattt catgactttg cagctcgtga aggatttcgc gcggttcagt cgagtattcg
      301 cggcgtctca tccaccggat cagccagccc tggaaaatgg ctcgaaacgt catctcctcg
      361 agcatcggcg ccaggatgac gcccgtaacg agcgccaagt agcccacatc gacagagaaa
      421 tgttcgcgca gcatcttttc gagaggatga ctgtttggtc gccagaccga aatcgccccg
      481 aactggacga gaaatacaac aggggtcgtg atcagcatcg cgactatgcc gacggccgct
      541 tgtctccacc accctgacaa ggatagtccg aggtcgtgca aatgagcccc cgaagtgcac
      601 ctaaggacaa gcggtgccaa gaccaacatg atgacctgcg ccaccgcgtt gacgaacatc
      661 atctcggtca tcgcaaacgt cggttcaatg gccttgtcgt cgacgtgttt ctcggctccg
      721 tcggctgcac ggtcgttctt ggcagccggc ggcggcgtct gacgcgccgg gctgcgtcca
      781 gtcgccaagg cgtagccttg cgagagcgtg aaccccacaa ctaaataaag caggacgatc
      841 agcaagaccg accacaatcc ccaggtcggc tcttggcgtg cgaccatggg cttttcgggc
      901 aaaatcggct gacgagtcaa caaccgccag atcaaccaac cccacgccat gaacatcgac
      961 gcgagtactg ccagcaggaa gaaagtcagc agcacgagca aaatctgggc tggtccgatt
     1021 ttcggcatgg ctcgagatcg gttgtggcgc gtgttcgcga tgggtcagga ttcgactcca
     1081 ctagatcgta ctcgcgtcgc gcgtaatcgg ccaccttgtg gagtcagcca ttcaactcta
     1141 gaatcggcaa atcgccgagc cctccgtccc ggtgagcggg cgagccgcct ggaaacctga
     1201 gacggcaatc gccgtggagt tttgatcgag ccatgcggga cgttgcccat cagctttcca
     1261 ttctgaaacg cggagtcgac caaatcgtcc cggaagtcga gttcctcaaa aagctcgacc
     1321 ggtcggtccg ggaaaaccgc ccgctccgcg tcaaatacgg catcgacccc accggcatcg
     1381 acgtccatct cgggcacacc gttccgctga ggaagctgcg gcaatttcag gaactgggcc
     1441 ataccgcggt catcatcatc ggcaactaca cggccttagt cggcgatccc tccggccgcg
     1501 acgagacccg cgccaagctt acgcaagagc aggttgaagc caacgccatc gactatctca
     1561 cccaggtggg gcgaatcatc gacctgagca aggccgaggt ccacaaaaac ggcgactggt
     1621 tttcgcgctg gacgtttctc gaggtcctcg acttgatgcg gaagatgacg atcggtcaga
     1681 tgacagcgcg cgaagacttc gccaagcgca tagccgccct caagccggtg taccttcacg
     1741 aatgtctata cccgctcatg caaggccagg actcggtcga gatcaaggcg gacgtcgagc
     1801 tgggcggtac cgagcagctc tttagcctgc tggtggcgcg cgacctccag caatcgcagg
     1861 ggcaagagcc gcaggtggcc atgaccatgc caatcttggt cggtaccgac ggcacgcgcc
     1921 gaatgggcaa gagcctgggc aattacatcg gggtggccga gcacgccgaa aaccaatttg
     1981 gcaagatcat gagcatcccc gacgagccga tgaggcaata ctttaccctc ttgaccgagc
     2041 tgccgatcac tgacgtcgac cgcatcttgg gaccggaaaa taacccacgc gatgccaagg
     2101 aaatcctggg caaggagatc gtcgaacagt atcacgggcc cgaagcggcc gaacgagccg
     2161 ctgccgaatt ccgccgccgc gccgccggcg aagatcccga ccagattccc gacgtcttcc
     2221 tcgccgccga caagctcgac tccgagggcc gaatctcaac cccagtgctg cttaaagaac
     2281 tcggtctgga atccagcacg tcgaacgctc gtcggctgat tgagcagggg ggggtgagcg
     2341 tcggtggcgg cgataggcgc gagacgatca ccgatgtcag ggccttgatc ggcgtcagcg
     2401 acggcttgat cgtgcgtatt ggtaagcgca agatcgcgcg cgtgaggtta gtctagacta
     2461 ctggcatggg catctaaatc gcggattcca atccaatttg agcgcaggcg atatggccct
     2521 gccacggaga cgatgcgacg atggctgagc acggtctggg cttgatcggc tgcggcatga
     2581 tcgccgaatt tcacacccgc gcgatcaacg agatcgccaa tgcgcgcgtc gtcggcgcct
     2641 ggagtcgcaa cgcggccaac gccgccaaga tcgccgggct ggccaaaggg ggttgtgcca
     2701 cgttcgacga cctggacgcc ctgttggcgc agcctggtct cgacatcgtc tgcatctgca
     2761 cgcccagtgg cgcccacatg gagtccgcgg ttcgggctgc gcgagcgggc aagcacgtgg
     2821 tggttgaaaa accgctggag atcaccctgc cgcggtgcga ctccatcatc gaggcttgtg
     2881 atgccgccgg tgtccggctt tgcaccatct tcccgtcgcg gtttacggcg gccaatattc
     2941 gcctcaagga ggcgatcgac ctcgggcgat ttggccgctt gaccctggga gacacctacg
     3001 tgaaatggtg gcggacgcag gagtattacg actctggagg ttggcggggc acctggcagc
     3061 tcgatggcgg cggcgcactc atgaaccagg cgattcacaa cgtcgatctg ctcacttggt
     3121 tgatgggtga cgtcgccaca atcaccgcct tgaccgccac gcttgcccac cagcgtatcg
     3181 aagtagaaga cacggcggtg gccgctctgc gattcaagaa tggagcgctc ggcgtcatcg
     3241 aagcagccac aagcgcgtac cccggcctct tgaaacgcac cgagatccac ggcgaccgcg
     3301 gctcggcccg ggtcgagcaa gacgacctca ccctctggga atttcaagaa aaggtcccga
     3361 gcgacaacga agtctatgcc gccatggcgg gccagaccgg cttcaaagct ggagcgagcg
     3421 atcctcgcgg catcactcat atcggccacc gcgaccagct gatcgacttc ctggaggcca
     3481 tcgacaccgg ccgcgctcct caggtcgacg gccgcgaagg acgcaagtcg gtcgagatca
     3541 tccgcgcaat ctaccgttcg gcgcagacac acacgtccgt tgaattgccc ctcgacgacg
     3601 aaggataaaa cgatgaccga gctcgagctg gctccaacaa gaagtcttat cattatagcg
     3661 ttgattgcgt gcgccttgtc ggcgccggct cgagggtcag acaagaatcc gccagcgccc
     3721 cttggcgccg acaaagatcg cgtgggcgac gaagtgcgcc gcatcccgtg gcgcggatca
     3781 agaataaagg ggagccccga tccacccgcg ccttataccg ccgagctggc cttcccgcac
     3841 ctcaagttca ataacccagt cgtgctggtt ccggccgcgg gaacaaacca gctctttctg
     3901 ggcgaggtca aggggcgtat ctattcgttt cccgaggatc ctggctgcac caaggccgac
     3961 cttgtgctcg atttagctcc gctgtatccc gactttaccg ccttctatgg actcgttttc
     4021 catccagagt ttgacaagaa tcgctacgtt tatctttgct acgtcctcaa gaacgatctg
     4081 gtcgatggct cggtggtttc gcggttcacg gtcagccgct cggacccacc ggtgatcgac
     4141 cctaagagtg agcaaattct tctgaagttt tggtccgggg gccataacgg tgcgtgtctc
     4201 gtcttcggca atgacggatg tctctatatc tccactggag acggtgcgtc accgacgcct
     4261 ccggacccga tgatgaccgg ccaagactgc tccgactttc tctcgagcgt cttgcgcatt
     4321 gacgtggacc atgccgaacc gggcaaagcg tatcgcattc cggccgacaa tccgtttctc
     4381 aatacgccgg gagttcggcc cgaaatttgg gcctatggct ttcgaaaccc ctg
//
LOCUS       DATAQL010001021         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_562237, whole genome shotgun sequence.
ACCESSION   DATAQL010001021 DATAQL010000000
VERSION     DATAQL010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562237"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1434
                     /locus_tag="VHS97_17665"
     CDS             265..1434
                     /locus_tag="VHS97_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3450086.1"
                     /translation="MRWSIRNQILIPLIGIQAVAVTAAALVMATLAARRSEREIIGRL
                     NDVVGTLGHGNFPYTSSVLARMHGLSGAHFIVRGDAGRVTGTSLPEIEDLPASLQTVP
                     AAGHVDSLAASPSVAVGDTRYFAVSVPSPGGLRGSSLIVLYPETAWRQARREAAMPAL
                     LLGVASLAAMVVVTSLIAHRMSRRIRRVERHVAAIAAGEFQQLEPGDRRDEVADLVAS
                     INQMCAQLEGMRQTIEQSERSRLMAQLAAGLAHQLRNSLTGARMSIQLHIKRQPAQPG
                     DETLAVALRQLALTEEQVKGLLSAGRLERSSPELFNVRQLLADVAGLVDPACQHARVA
                     FSQMCEDEEGPLDIKADRSSLRAAVLNLTMNAIEAAGQGGTVSLRLSHENGKCRS"
     gene            1422..1652
                     /locus_tag="VHS97_17670"
     CDS             1422..1652
                     /locus_tag="VHS97_17670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450087.1"
                     /translation="MSIVVVDSGPGPPSELADEVFEPFVTSKKEGVGLGLALAHQVAV
                     DHGGQLSWTRAAGATRFLLTVPRANGTHKGAE"
     gene            1649..>2267
                     /locus_tag="VHS97_17675"
     CDS             1649..>2267
                     /locus_tag="VHS97_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450088.1"
                     /translation="MSQILIVDDEASICWAFRESLADLGHQVEVAASAEEGLRIAGTS
                     SLDAVVLDVRLPGMDGLTAMRAFRDHVGPIPIIVITAFGNLETAVRALEGGAFDYLVK
                     PFDLDQATTVVKRALEKRQTRALTGAPSPVDTQTLIGSSPPMQELFKSIALVAPTDVP
                     VLITGESGTGKELVARAIHRHGPRRDGPFLPICLAALSPSLVESEL"
BASE COUNT          423 a          707 c          719 g          418 t
ORIGIN      
        1 gaaggcaaag acaagaccct tacggtcggt cgtttcgcca aggagacggc agtgcaccta
       61 tcccgccaaa gctttctcgt acctgcacat tgattatgga cgtgcaaggc gatcaaacta
      121 ttcgctacga gatcgactag aattttgaat ggtaaacgaa cgttttcgtt ttccctaact
      181 ccctcagccg tttgcaaatt cggggcctcg ggcctccggc agcctaatgc gggaccgttt
      241 cgtcgcaggc caaaaaattc gccgatgcgc tggtcaatac gcaaccaaat tttgatcccc
      301 ctgatcggca tccaggccgt agccgtgaca gccgcggcac tcgttatggc gactttggcg
      361 gccaggcgga gtgaacgcga gatcatcggc aggctcaacg acgtcgtggg tacgctcgga
      421 catgggaatt tcccctacac cagtagtgtg ctggccagga tgcacgggct ttctggggct
      481 cactttatcg tccggggaga cgcaggccgg gtcacgggaa ccagcctacc ggagatcgaa
      541 gacttgccag cctcgctcca aacggttccg gctgcgggcc atgtcgactc actcgcggca
      601 tcaccgtcag tcgcggtcgg agacactcgc tacttcgcgg tgtcggtgcc gtcaccgggt
      661 ggacttcgag gcagctcact gatagtgctt taccccgaga cggcctggcg ccaggcacgg
      721 cgggaagctg ccatgcccgc cttgttgctc ggcgtcgcgt cgctcgcggc gatggtcgtg
      781 gtaacaagct tgattgccca ccgcatgagc cggcgtatcc ggcgtgtcga gcggcacgtg
      841 gccgcgatcg ccgccgggga gttccagcaa ctcgaacccg gcgaccggcg tgatgaagtc
      901 gccgatctgg tggcgtcgat caaccagatg tgcgctcagc tcgaaggcat gcggcagacg
      961 atcgagcagt ccgaacggtc ccggttgatg gcccagcttg cggcggggct ggctcatcaa
     1021 cttcgtaact cgctcaccgg cgctcggatg agtattcaac tgcacattaa acgccagccg
     1081 gcgcagccag gcgacgagac ccttgccgtc gccctacgac agctcgcact cacggaagag
     1141 caggtcaagg gactgctctc ggctggacgg ctcgagcgga gttcacccga attgttcaac
     1201 gtgcgccagc tcttggcgga tgtggccggt ttggtggatc cggcatgcca gcacgcgcgc
     1261 gtcgcttttt ctcagatgtg cgaagatgag gaaggaccgc tcgatatcaa ggccgaccgg
     1321 tcgagcttgc gcgcggctgt attgaacctg accatgaacg cgatcgaggc ggccgggcag
     1381 gggggtaccg tgagcctgag gctttcgcac gagaacggga agtgtcgatc gtagtcgttg
     1441 acagcggccc aggaccgccc tcggagctgg cggatgaggt attcgaacca ttcgtcacga
     1501 gcaaaaagga aggtgtgggg cttggcctgg cgctcgcgca ccaggtcgcg gtcgaccacg
     1561 gcgggcagct atcgtggacc cgagccgccg gcgccacccg ctttctcctt accgttcccc
     1621 gggccaacgg aacacacaaa ggggccgaat gagccaaatt ctgatcgttg atgatgaagc
     1681 gagcatctgc tgggcgtttc gcgagtcgtt ggccgatctc ggacatcagg tcgaagtggc
     1741 cgcttccgcc gaggaaggcc tgcgaatcgc ggggacgagc tcgctcgacg cggtcgtact
     1801 cgacgtccgg ctgccgggca tggatggact gaccgcgatg cgcgcgttcc gcgaccacgt
     1861 cggcccgatc cccatcatcg tgatcaccgc cttcggcaac ctcgagactg cggttcgggc
     1921 gctcgagggc ggggctttcg attatctggt caagcccttc gacctcgacc aggcgacgac
     1981 cgtcgtgaaa cgcgcgctcg agaaacgaca aacccgtgcg ctcacggggg cgccgtcgcc
     2041 tgtggatacg cagaccctga tcggttcgtc ccccccgatg caagagctct tcaagagcat
     2101 cgcactggtc gctccgaccg atgtgcccgt gctgatcacg ggcgaaagcg gaaccggtaa
     2161 ggagctggtc gcccgggcaa tccaccgtca cggaccccga cgcgacggcc cttttttgcc
     2221 gatctgcctg gccgctctca gtcccagcct ggtcgagagc gaactct
//
LOCUS       DATAQL010001022         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1019719, whole genome shotgun sequence.
ACCESSION   DATAQL010001022 DATAQL010000000
VERSION     DATAQL010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019719"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..>2193
                     /locus_tag="VHS97_17680"
     CDS             388..>2193
                     /locus_tag="VHS97_17680"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEX3450089.1"
                     /translation="MANLAHPLQGLTQFGLLTFSVFVLTLPLTAAAQAPPSDPATQTR
                     PAAAARGGDLASKTSATSHPLDPLQPAEIELAVAAIRKERSLSESVRFVTISLKEPSK
                     EVGRRTSATAGSTREAFLILLDSSTGRGYEAVVDLGNGAVKRYKALPEGVQPPIMIDE
                     FMECEDAVKRAPEFREAMKKRGIDDVGLIMVDAWSAGHYGNEPPEDRGKRLVRALCWI
                     RTEPRDNGYARPIENIVAVVDLNRKQLVRVEDYGVIPLPRQAGNWAREYTPDRRAGLK
                     PLEVTQSKGASFKVEGHEVQWDKWSFRIGFNPREGLVLYTVGYDGRPVLYRASIAEMM
                     VPYADPKQSSYHKNVFDLGEYGVGMMANSLALGCDCLGTIQYFDGHMADSKGRVVTIK
                     NAICLHEEDFGMLWKHTDWRTSQSDVRRSRRLAVSMVATVGNYDYGFYWYLYQDGTIQ
                     MEVKLTGIMNTTALKPGEPSRYGTEVAPGLNAPYHQHYFNARLDFAVDGEANTVQEVN
                     THSVPAGPDNPFNSAFVAETTPFLKELEAQRTTNTPSSRFWRVVNPGRKNGMGQPVAY
                     RICPGENTLPYALAGSSLLKRATFLTRNLWVTPFDP"
BASE COUNT          455 a          701 c          646 g          391 t
ORIGIN      
        1 ctctgacctc ccagaccgac attttcacgc agttcgcgcg gtcgctcggt ggcggagccc
       61 cgcccccaat ccgcctgcac gaggcttgcc gcattaccga gatcgccatc aaggcgcaac
      121 atgccgccga cggtaaggcc atggtctcgt tgggcgattc gctctaccgc gcggtctgag
      181 tcctgtaacg acaggcaaca aaggtcgcca tcaagacgct gcaggcggca gtcaccaagg
      241 ccgtcgtagt tgccagcggc tctccataac acatcgcctg aggtcccggg aactgactag
      301 caactttgca aacgccatca agtcggttaa ccttcagact caaactactg caccaaaccg
      361 atcaccttgc tgctgtggaa cgcgttcatg gcgaatttgg cacacccgct ccagggcctt
      421 acgcagttcg ggcttctgac cttttcggtt ttcgttctga cccttccgct gacggcggcg
      481 gcccaggctc cgcccagcga tccagcgact cagacccggc cggcagcggc agctcgcggc
      541 ggtgacttgg catccaagac ctccgccacg agtcacccgc tcgatccgct ccagcccgct
      601 gagatcgaac tcgccgtggc cgcaatccgc aaggagcgtt cgctcagtga gagcgtgcga
      661 ttcgtgacca tcagtctgaa agaaccgtca aaagaagtcg gccgtcgaac aagcgccacg
      721 gccggctcga cccgcgaggc attcctcatt ctgctcgaca gttctacggg gcggggctac
      781 gaggcggtcg tcgatctggg caacggcgcc gtcaagcggt acaaagcgct cccagagggt
      841 gtacagccgc caatcatgat cgacgaattc atggaatgcg aagacgcggt caagcgcgcg
      901 cccgaatttc gcgaggcgat gaagaagcgc ggcatcgacg acgtcggtct catcatggtc
      961 gatgcctggt cggccggcca ttatggcaac gagccgcccg aggatcgggg caaacggctc
     1021 gtgcgtgcgc tctgctggat ccgaacggag ccgcgggaca acggttacgc ccggccgatc
     1081 gagaatatcg tcgcggtcgt cgatctcaac cgcaaacaac tcgtacgagt cgaagactac
     1141 ggcgtgatac ccctgccgcg ccaggccggc aactgggctc gcgaatatac ccccgatcgc
     1201 cgcgcagggc tcaagccgct cgaggtgacg cagtcaaaag gagcgagctt caaagtcgag
     1261 gggcacgaag tccagtggga caagtggtca ttccgcatcg gcttcaaccc gcgcgaaggc
     1321 ctggtgctct ataccgttgg gtacgacggg cgcccggtgc tgtaccgcgc gtcgatcgcc
     1381 gagatgatgg tcccgtatgc cgatcccaag cagtcgtcat accacaaaaa cgtcttcgat
     1441 cttggcgagt atggcgttgg catgatggcc aactcgctgg cactgggctg cgattgcctg
     1501 ggaaccatcc agtatttcga tggccacatg gccgatagca aaggccgggt cgtcacgatc
     1561 aagaacgcga tttgtctgca cgaagaagac ttcggcatgc tctggaagca caccgactgg
     1621 cgcaccagcc agtccgatgt gcgccgatcg cgccgcctgg ctgtgtcgat ggtcgccacg
     1681 gttggcaact atgactacgg gttctactgg tatttgtacc aggacggcac gatccagatg
     1741 gaagtgaaac tcacggggat catgaatacg accgcattaa aacccggcga gccgtctcgt
     1801 tacggcacgg aggtggcgcc gggattgaac gccccgtacc accagcatta cttcaatgcc
     1861 cgactcgatt tcgccgtcga cggcgaggca aacaccgttc aagaggtcaa tacacacagc
     1921 gtgccggccg gtccggacaa tccgttcaac agcgcctttg tcgccgagac gacaccgttt
     1981 ctcaaggagc ttgaggccca gcgcaccacc aacacgccct cttcgcggtt ctggcgggtc
     2041 gtcaaccccg ggcgcaagaa cggcatgggg caaccggtgg cataccgaat ctgccccggc
     2101 gaaaatacct tgccgtatgc gttggccggt tcgtcgttgt taaagcgggc gacgttcttg
     2161 acgcgaaacc tatgggtcac acccttcgac ccc
//
LOCUS       DATAQL010001023         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3155940, whole genome shotgun sequence.
ACCESSION   DATAQL010001023 DATAQL010000000
VERSION     DATAQL010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3155940"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VHS97_17685"
     CDS             <1..298
                     /locus_tag="VHS97_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450090.1"
                     /translation="REFTREGVPLHLVTREQVAKPPREILEIEGITTVGLADLIEMKL
                     ASGMRNMLRAQDLADVIGLIRHNGLTSEYAKHLDKSLRPAFRKLVKAIQQEGSC"
     gene            413..1294
                     /gene="sucD"
                     /locus_tag="VHS97_17690"
     CDS             413..1294
                     /gene="sucD"
                     /locus_tag="VHS97_17690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX3450091.1"
                     /translation="MSILVDKNTRLICQGITGKAGAFHAEPCKAYGTNLVGGITPGRG
                     GTTTLGVPVFDTCSDAVAATGANATMIFVPPPFAADAIMEAADAGITLIVAITEGIPV
                     LDMARAVAFLKAHYPNVRLIGPNCPGIITPGACKIGIMPGYIHKPGRVGVVSRSGTLT
                     YEAVWQLTNLEIGQSTCVGIGGDPVNGTSFVDSLLLFQNDPDTDGVLMMGEIGGNAEE
                     QAASLKASGRFTKPLAAFIAGQTAPTGKRMGHAGAIISGGSGKASDKIAALEAAGIKV
                     AKSPADMGVTLQAALKG"
     gene            1303..1893
                     /locus_tag="VHS97_17695"
     CDS             1303..1893
                     /locus_tag="VHS97_17695"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3450092.1"
                     /translation="MSPPEDVEPLPDRSNPWQTVARRFVYENPWIRVREDQVIRPDGL
                     PGIYGVVEFKNRAVGVLPVDHDGSIWLVGQYRYPLQAYSWEIPEGGSPDSESAEATAI
                     RELREETGLLAGRMELVLTSHLSNSVSDELGYLFRATELTHGANDPEGTERIAVRRFE
                     WDLAWRMLKSGEITDSLSVMALMHEAIRRIEGAKTK"
     gene            complement(1968..>2705)
                     /locus_tag="VHS97_17700"
     CDS             complement(1968..>2705)
                     /locus_tag="VHS97_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX3450093.1"
                     /translation="PPAPPKPVSPGAGSGSGPALLGPNSAASPSAWETDSQDGVQTTA
                     TPSSRVRRASLNERLRPFLDDSTANDLMYPNKVDRPWRYIVMHHSASASGNYDQIDAE
                     HRKILGINGCGYHFVIGNGTGSSDGQIEVSQRWINQKQGAHTRNARTHDADEYGIGIC
                     LVGDFDKQPPSPKQIAATKALIEYLNKRYNIAPDGLRTHSHLAATTTVCPGKFFPSEA
                     MLMVTKDAMNERPVRATWKVVRDTTQK"
BASE COUNT          517 a          775 c          891 g          522 t
ORIGIN      
        1 ccgcgaattc acgcgcgaag gcgtgcctct ccatctcgtc acgcgcgagc aggttgccaa
       61 accgccgagg gaaatcttag agattgaagg aatcacgacc gtcggtctcg ccgatctgat
      121 cgagatgaaa ctggcgagcg gcatgcggaa tatgctgcgg gcccaggatc tggcggacgt
      181 catcggtctg atccggcata acggtctcac aagcgaatac gcgaagcatc tcgacaagtc
      241 gctacggccc gcttttcgaa agctcgtcaa ggcgatccag caagaaggga gttgctgaga
      301 gccggtaatt cggcttcatt agccatttac ctgaggcaat caatcgaagt tcattgcttt
      361 ttcgtgcaag ggtaaacatt cacgagaagt tagcgagaag acgggacgac tcatgagcat
      421 tctggtcgac aagaacacgc gcttgatctg tcagggcatt accggcaaag ccggcgcgtt
      481 tcacgcagag ccgtgcaaag cgtacggcac aaacctggtc ggagggatca caccgggtcg
      541 gggaggcacg acgacgctgg gcgttcccgt cttcgatact tgctcagacg cggttgccgc
      601 caccggagcc aacgccacaa tgatcttcgt gccccctccg ttcgctgccg acgcgatcat
      661 ggaggcggct gatgccggga tcactttgat cgtggcgatc accgagggga ttccggtgct
      721 cgacatggcg cgtgcggtcg ctttcttgaa ggcgcattat cccaatgtcc ggctgatcgg
      781 gcctaactgt ccggggatca tcacccccgg cgcctgcaag atcggcatta tgccgggcta
      841 tatccacaag ccgggccggg tgggcgtggt cagtcgctcg gggaccctga catatgaggc
      901 cgtctggcag ctcaccaact tggagatcgg gcagagcacg tgcgtcggca tcggtggcga
      961 cccggtcaac ggcaccagct ttgtcgactc actccttctc tttcaaaacg acccggacac
     1021 cgacggtgtg ctgatgatgg gggagattgg cggcaacgcc gaggaacagg ctgctagcct
     1081 gaaggcgtcg ggccggttca cgaaaccgct cgccgccttc atcgccggcc agacggcccc
     1141 gaccggaaag cggatggggc acgccggcgc gatcatttcg ggagggtccg gcaaggcttc
     1201 cgacaagatc gcggctctcg aggcggccgg catcaaggtc gccaagagcc cggccgacat
     1261 gggcgtcact cttcaggccg cgctcaaggg gtaatcgacc ggatgagccc gcctgaagac
     1321 gtcgaaccgc ttcccgaccg gtcgaacccg tggcagacag tcgcgcgccg gtttgtatat
     1381 gaaaatccgt ggatccgcgt ccgcgaagac caggtgatcc gcccggacgg cttgccgggc
     1441 atctatggag tggtcgagtt caaaaaccga gcggtcggag tcttgccggt cgatcacgat
     1501 gggtcgatct ggctggtcgg ccagtatcgt tatcccctcc aggcctattc gtgggagatc
     1561 cccgagggtg gaagccccga ctctgaatcg gcggaggcga ccgcgattcg agagctgcgc
     1621 gaggagaccg gtctcttggc gggccggatg gagctggtgt tgacgtccca cctttccaac
     1681 tcggtcagtg acgagctggg ctatctcttc cgggcgaccg agctcacaca cggtgctaat
     1741 gatcccgagg gaaccgagcg gatcgcggtt cggcgatttg agtgggacct agcctggcga
     1801 atgctcaaaa gcggtgagat taccgactcg ctcagcgtca tggccctgat gcatgaggcc
     1861 attcgccgta tcgaaggggc caaaacaaag tgagcgagtc cgcttccctg cgggctcgaa
     1921 actgcacgag attcaagtgg agtgggatcg cacgcaggcg tcgtcggcta cttctgggtg
     1981 gtgtcgcgga cgaccttcca ggtcgcgcgg accggccgct cgttcatcgc gtctttggtg
     2041 accatgagca tcgcttcgct ggggaaaaac ttgcccgggc acaccgtcgt agtcgccgcc
     2101 aaatgggagt gagttcgcaa gccgtcagga gcgatgttgt agcgcttgtt gagatattcg
     2161 atcaaggcct tggtcgcggc gatctgcttg ggcgagggag gctgcttgtc gaaatcaccc
     2221 acgaggcaga tgccgatgcc gtattcgtcg gcgtcgtgag tgcgtgcgtt gcgagtgtgg
     2281 gcgccctgct tctgattgat ccagcgctgg gatacttcga tctggccgtc gctgctgccg
     2341 gtgccgttgc cgattacgaa gtgatagccg cagccgttga tgccgaggat cttccggtgc
     2401 tcggcatcga tctgatcgta gttgccgctg gccgaggcac tgtggtgcat gacgatatag
     2461 cgccaggggc ggtcgacctt gttcgggtac atgagatcat tagccgtgga gtcatctagg
     2521 aagggccgga gccgctcatt caagctggca cgtcgcactc gggaagaggg cgtcgcggtg
     2581 gtttgcactc catcctgtga gtcggtttcc cacgcagatg gagaagcagc gctgttgggc
     2641 cctaagaggg ctggtccaga acccgaacct gcgcccgggc tgaccggctt gggcggagca
     2701 ggggg
//
LOCUS       DATAQL010001024         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_562576, whole genome shotgun sequence.
ACCESSION   DATAQL010001024 DATAQL010000000
VERSION     DATAQL010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562576"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VHS97_17705"
     CDS             complement(<1..249)
                     /locus_tag="VHS97_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450094.1"
                     /translation="MNLRDLIEALSAPSAYFHPVETVDVRQTHISVVFLAGSFAYKVK
                     KPVGLAFIDYGTLERRHHFCEEEVRLNRRLAPEVYLGVV"
     gene            complement(326..925)
                     /locus_tag="VHS97_17710"
     CDS             complement(326..925)
                     /locus_tag="VHS97_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450095.1"
                     /translation="MNGSLKIAIADDELDMRDYFQQILPLLGHQVIAVARDGRELVDL
                     CATTRPDLVITDIKMPDMDGIEAATRIYQNAPIPVILVSAYHDPEFIRRAEADHILAY
                     LVKPIKQADLEPAIGIAMRRFEQFQALRKETSDLKQALEDRKVIEKAKGILMKKAGLD
                     EHDAFRRLQKLASDKNRKLIDIAQMILTAEEALEPPRRT"
     gene            complement(922..>2167)
                     /locus_tag="VHS97_17715"
     CDS             complement(922..>2167)
                     /locus_tag="VHS97_17715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450096.1"
                     /translation="KLKAADPNAAVIVVTGYSDLDGAIAALRQGATDYILKPFNPDFL
                     RTSLGRIAERKQLAQAKERSDAAFRHLVEAADCMIVILRPDHSIAYYSPFAEKLTGYS
                     AEDVKGSDYLTLLLPESDHHAVADEFKRVIAGRHNRGLENAVICRDGSRRWIVWNARY
                     LPDYEDGPAILSVGHDITFVKHAQERALQSERLAAIGQVVTGLAHESRNALQRCQACL
                     EMLALTVRDRPESLELISRLQKAQDHLHHLYEDVRSYAAPIKLEKVPCDLRTVWRESW
                     AHLELARKDKQGRLVETLDGVDLSCAVDPFRLGQVFRNILENALAAGSPPVAIDVRAA
                     AAELDGQPALQIAVRDNGPGIALDQRTKVFDPFYTTKSKGTGLGLAIAKRIIEAHGGQ
                     LAIGEAHQTGAVFLITLPRGTP"
BASE COUNT          393 a          615 c          719 g          440 t
ORIGIN      
        1 gactaccccg agatagacct caggcgccag ccggcggttc aagcggacct cttcctcgca
       61 gaagtgatgc cgccgctcta gcgtcccgta gtcgatgaag gccagcccga cgggcttttt
      121 gaccttgtag gcgaacgagc ccgccagaaa gacgacggag atatgcgtct ggcggacgtc
      181 cactgtttcg accgggtgga aataggcgga cggggccgaa agagcctcga tcaaatcccg
      241 gagattcatc gaagtctaac ctcaagttca agttcgatct tgccgcgtcg gctcaaccgg
      301 gcgggcgccg ccgtgccatg gggaatcaag tccggcgggg cggctcgagg gcctcttcgg
      361 cggtcaggat catctgggcg atgtcgatga gcttgcggtt cttgtcactg gccagcttct
      421 gaaggcgacg gaaggcgtcg tgttcgtcga ggccggcctt cttcatgaga attcccttgg
      481 ccttctcgat caccttgcgg tcctcgagtg cctgcttcag atccgaggtt tccttgcgga
      541 gcgcctgaaa ctgctcgaat cggcgcatgg cgatgccgat cgccggctcg aggtcggcct
      601 gcttgatagg cttgaccaga tacgccagga tgtggtcagc ctcggcgcgg cggatgaact
      661 cgggatcgtg ataggccgac accaagatga ccggaatcgg agcgttttga tagatgcgcg
      721 tggccgcctc aataccgtcc atgtcgggca ttttgatgtc agtgatcacc aggtcgggac
      781 gggtggtggc gcataggtcc actagctcgc ggccgtcccg ggccacggcg atcacctgat
      841 ggcccaagag cgggagaatc tgctgaaaat aatcgcgcat gtcgagctcg tcgtcggcta
      901 tggcgatctt gagcgaaccg ttcatggtgt ccctcgcggt aaagtgatga gaaagacggc
      961 tcccgtctga tgtgcttcgc ctatcgcgag ctggccccca tgtgcttcga tgattcgttt
     1021 ggcaatagcc aggccgagac cggtgccctt ggacttggtg gtgtaaaacg ggtcgaagac
     1081 tttcgttcgc tgatcgagag caattcccgg cccgttgtcg cgtacggcga tctgcaaggc
     1141 gggctggccg tcgagttcgg cagcggcagc gcggacgtcg atagcaacgg gtggcgagcc
     1201 ggcggccagg gcgttctcca gaatattccg aaagacctgt ccgagtcgaa aagggtcgac
     1261 tgcgcagctc aggtcgacac cgtccagcgt ctcaactaag cggccctgct tgtccttgcg
     1321 ggccagttcg aggtgggccc atgattcgcg ccagaccgtg cgcaggtcgc acgggacctt
     1381 ctcgagcttg atcggagccg cgtagctgcg aacatcctcg tagaggtgat ggagatggtc
     1441 ttgagccttc tgcagccggc tgattagttc gagcgactcg ggacggtcgc ggacggtcaa
     1501 ggcgagcatc tcgagacacg cctggcagcg ctgcaaggca ttacggctct cgtgggccaa
     1561 cccggtgacg acctggccga tggcggccaa tcgttccgac tggagtgccc gctcctgagc
     1621 gtgcttgacg aacgtgatgt cgtgaccgac gctcaaaatt gccggaccgt cttcgtagtc
     1681 aggcaggtac cgtgcgttcc agacaatcca gcggcgtgag ccgtcgcggc agatgaccgc
     1741 attctcgagg ccgcgattgt ggcgacccgc gatgaccctt ttgaactcgt cggcgaccgc
     1801 gtggtggtcg gattcaggca acagcagcgt caggtaatcg cttcccttga cgtcttcggc
     1861 cgagtagccg gtgagctttt cggcaaacgg actgtagtag gcgatcgagt ggtcggggcg
     1921 gaggatcacg atcatacagt cagcagcctc gaccaggtgg cggaatgccg catcgcttcg
     1981 ttccttggcc tgggcaagtt gtttccgctc ggcgattcgg cccagactgg tgcgcaggaa
     2041 gtcagggttg aacggtttca ggatgtagtc ggtcgcgcct tggcgcaaag cggcaatggc
     2101 tccatcaagg tcggagtagc cggtcacgac gatgactgcg gcgttgggat cggcagcctt
     2161 gagtttg
//
LOCUS       DATAQL010001025         5164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3767949, whole genome shotgun sequence.
ACCESSION   DATAQL010001025 DATAQL010000000
VERSION     DATAQL010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767949"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VHS97_17720"
     CDS             <1..346
                     /locus_tag="VHS97_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450097.1"
                     /translation="VPVVLDQGKFDVGRFANELADGMLNRLVRVELSKGVKDKGKMHY
                     QLRIDNASPLVLNGLAALGTTSKPDEMPKILSGICLSPRRSMTVPASEDVVKALALKK
                     GIRLVGLDLSGL"
     gene            693..878
                     /locus_tag="VHS97_17725"
     CDS             693..878
                     /locus_tag="VHS97_17725"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEX3450098.1"
                     /translation="MSKKALSTLDDGRAVPAAPSRDLDLDHVRTAIQGIRYGEVRVII
                     QDGVIVQIERVEKQRLR"
     gene            997..2199
                     /locus_tag="VHS97_17730"
     CDS             997..2199
                     /locus_tag="VHS97_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3450099.1"
                     /translation="MPSNDRKGGPEPAPTASRPDSSVNLVRRVPHCGPGFTLIELLVV
                     IAIIAVLIALLLPAVQAAREAARRVSCINNLKQIGLALHNYHQINDVFPPGGFPAYIP
                     NKNNGNNACPSAQTRLLPFLEQQPLYDALNWSLTVINDVAPGNGYGPYANSTVTITRL
                     TAFLCPSDTPPNWNLTSASQPLPSYRAPGNNYFASVGSSLEFASRQTSGPPNGPFSYV
                     GDIGRICGIRNVQDGTSNTIGFGEWRIGDGNSSLVSIPSDVIFIGTLPSGTARNNGTL
                     TMPNPILVASFPAWIQSCVGASAGDRTNHTSALGEAWIFDITGLSFGNVLLPPNPKTP
                     NCDSSTVASNTLQNPGMWGLSSYHPGGANVVFLDGSVKFLKDSTSQQTLWALGSIAQG
                     EVLSSDSY"
     gene            2302..4941
                     /locus_tag="VHS97_17735"
     CDS             2302..4941
                     /locus_tag="VHS97_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3450100.1"
                     /translation="MSLKPVLLCSLAVVSIAAGPTPGRPERHFDAESVEYFEKKVRPL
                     LVNNCNNCHSATTNSKGGLRIDDRNGLLQGGNRGPAVVPGDPENSILIQAVLQTEDDL
                     KMPPKKRLSAEEVAVLTKWVKDGAAWTEVDAAVVTGNVNPKYDKLRKEHWAWQPLKEA
                     KAPVVRDAFWPRSSIDRFVLAKLESAGLNPVRDADKLALIRRVTFDLAGLPPTPEEID
                     AFLADNSSEAFKHVVDRLLASPAYGERWGRHWLDVARYGESTGSSRNLPMPHAWRYRD
                     YVIEAFTRDKPYNAFIQEQIAGDLLPATSQSARDEQRIATGFLAIGVKDVNQRFTVRF
                     IMDNVDEQIDAVSRAFLATTVSCARCHDHKFDPIPTSDYYALAGIFRSTELCAGLRNK
                     MGGGGLDYYDSAMLVRLGPEAKPDPQSAEKVAAATKAYEAAKKEFEAIRGTPKGLALA
                     ANGQPTQRPFRLKMIKLQNELLALTDPANGKVAFGVRDAKTVADTEIRVRGEAEKLGP
                     VVPRGFLSIVPIADVPKINSSQSGRLELAQWITSAKNPLTPRVMANRVWQHLFGKGLV
                     QSVDNFGVTGDVPSHPELLDYLAASFVREDWSIKKLVRKVVLSRTYALASDVSESNFA
                     VDPANRLIWRHSPRRLSAEEIRDATLAAAGKLDLGRPDGSPAKDLKVTEMANNGPLAR
                     RIGEQASRSTLRSIYLPLLRGVTPTSLDVFDFAEQGMVTGSRDTTTVATQALYLLNDP
                     FVRRQSLALAERLLDQTEADCANRIDRAYRLTICRPASAAEIERASRYLASYESSVRD
                     LVAFTPPAPALRPNTVVAAVDADTSAKAAAKRPAPPVNPDEVEQVEAPVAEEVIAAAN
                     PETAAWASFCQALLGSAEFRYIK"
BASE COUNT         1034 a         1556 c         1591 g          983 t
ORIGIN      
        1 agtcccggtc gtcctcgacc aagggaaatt tgatgttggc cgctttgcca acgagctggc
       61 tgatggcatg ctcaatcgct tggtccgggt tgagttgagc aagggtgtca aagacaaggg
      121 aaagatgcat taccagctgc gcattgacaa tgcgtcgcca ctggttttaa acggcctggc
      181 ggcgctgggg acgaccagca aacccgacga gatgcccaag atcctgtcgg gcatctgcct
      241 ctcgccgcgg aggagtatga ccgtcccggc cagtgaggac gtggtcaagg cgttagcgct
      301 caagaagggg atcaggctgg tgggccttga cttgagcggt ctctgatcgt gcccagctga
      361 tggccgcctc tcgcttgacc ggtgcgattt gcgttaaaat aacaaacgac ttccgctccc
      421 gtgatcctca catccggggg cggttttttt taccattact tgcggacgtt accgagttgg
      481 gatcctcggc cggcgctgaa tacttttggc acgcacaccc caagaaccct attcctgatc
      541 aaattgatct aatttgtcga tattagtctt gacgcgggat tttggcgttg aaagaatgag
      601 taactcgtgg cgaaacgttg gtgatgtggg cagcgattcg agtaggtcgt tgccaatccg
      661 ttatggctgc acggaactgt ggcgaggtga atatgtcgaa gaaggctctt tccactcttg
      721 atgatggacg agccgtccca gccgcaccct cccgcgatct ggatttggat cacgttcgga
      781 cagccattca aggcattcgc tacggtgaag tccgtgtgat catccaggat ggagttatcg
      841 tccagattga gcgagtcgag aagcagcgac tccgttaaga gagttgattc gctcggtcca
      901 gtatcgccca ctcgtcatcg agaggcgtgt tgatctccca acgccgcatg tcgccgactg
      961 gtcacaccag aggcgggcgg attttcggag ttgcacatgc cctccaacga tcggaaaggc
     1021 gggcccgaac cggcccccac agcctcgcgc ccggactcct cagttaacct ggtgcggcgc
     1081 gtgcctcact gcggccccgg gttcacgctg atcgagctac tggtggtgat cgcgatcatc
     1141 gccgtgctga tcgcgctttt gctccccgct gtgcaggccg cgcgcgaggc ggcgcggcgg
     1201 gtgtcttgca tcaataacct caagcagatc ggactggcgc tccacaatta tcaccagatt
     1261 aacgacgtgt ttccgccggg gggcttcccc gcttacattc ccaacaagaa taacggcaac
     1321 aacgcgtgcc cgagcgctca gacgcggctc ttgccgtttc tcgagcagca gccgctctac
     1381 gatgcactca actggtcgct cacggtgatc aatgacgtcg cgccgggcaa cggctatggg
     1441 ccgtacgcca attcgacagt cacgatcacg cgattgaccg cgttcctttg cccgtccgat
     1501 acacctccga actggaactt gaccagtgcg tcccaacctt tgccgagcta tcgggccccc
     1561 ggaaacaact acttcgccag cgtcggttcc agcctcgagt tcgcgagccg ccagacctcg
     1621 ggtccgccca acgggccctt ttcgtacgtc ggcgacatcg gcagaatctg cgggatccgc
     1681 aatgtccaag atgggacgag caacacgatc ggctttggcg agtggcggat cggcgacggt
     1741 aactcgtcgc tggtgtcgat cccttccgat gtgatcttca tcggcaccct gcccagcggc
     1801 acggcccgaa acaatggaac gctgacgatg ccaaatccca tcttggtcgc gagcttcccg
     1861 gcatggatcc agagctgcgt gggcgcgtcg gcgggcgatc ggacgaacca cacaagcgcg
     1921 cttggtgaag catggatctt cgacatcacc ggcttgagtt tcggcaacgt cttgctgccg
     1981 cccaacccga aaacgcccaa ttgcgactcg tcgaccgtgg ccagcaacac tctgcagaat
     2041 cccggcatgt ggggactctc cagctatcac cccggcggtg caaacgtggt ctttctcgat
     2101 gggtcggtga agttcctcaa ggacagcacc agtcagcaaa ctctctgggc gctcggctcg
     2161 atcgcgcagg gcgaggtcct ctcctccgat tcgtactgaa tcggccggcg gggcggcgcg
     2221 cgccatcggg gatgagatcc taatgcattt tccgctgaca ttgtcgcaat tacatataag
     2281 aaatgaaagg atgattctta gatgtcgttg aagccggttc ttctctgttc actcgcggtt
     2341 gtgtcgatcg cggccgggcc gacgccgggc cggcccgaac gtcatttcga cgcggaatcg
     2401 gtcgagtatt ttgaaaagaa agtccggccc ctgctggtga ataactgcaa caactgccat
     2461 tccgcgacca cgaattccaa gggagggctg cgcatcgacg accgcaacgg cttgctccaa
     2521 ggggggaacc gagggcccgc cgtcgttccc ggtgatcccg agaacagcat cttgatacag
     2581 gcggtcctcc agacggagga cgacctgaag atgccgccga aaaagcggct atccgcggaa
     2641 gaagttgccg tgctcacgaa gtgggtcaag gacggcgcgg cctggacgga ggtggacgcg
     2701 gcggtcgtca ccggaaacgt caatccgaag tacgataagc tccggaaaga gcactgggcc
     2761 tggcagccgc tcaaagaagc caaggctccc gtggtacgcg atgccttttg gccgcgctcg
     2821 agcatcgacc gcttcgtact ggcgaagctc gaaagcgcgg ggctcaatcc cgtccgcgac
     2881 gcggacaagc ttgccctgat acgccgcgtc accttcgact tggccggctt gcccccgaca
     2941 cccgaagaga tcgacgcctt cctggcggac aactcttccg aggcattcaa gcacgttgtt
     3001 gaccgactgc ttgcctcgcc cgcgtacggc gagcgttggg gcaggcactg gctggacgtt
     3061 gcccgctatg gagaatcaac cgggtcgtcg cgcaacttgc cgatgcccca cgcctggcgt
     3121 tatcgcgact acgtcatcga ggcattcact cgcgacaaac cgtacaacgc gttcatccag
     3181 gagcaaattg ccggtgactt gctgccggcg acctctcaga gcgcgcgcga tgagcaaaga
     3241 atcgcgacgg gattcttggc gattggcgtc aaggacgtga atcagcgctt tacggtgcgg
     3301 ttcatcatgg acaacgtcga tgaacaaatc gatgcagtga gccgcgcatt cctggcgacg
     3361 acggtgagct gtgcccgttg ccacgaccat aagttcgacc cgattcccac cagcgactac
     3421 tatgccctcg cgggcatctt ccggagcacc gagctgtgcg ccggcttgcg caacaagatg
     3481 gggggcggcg gcctggacta ttatgattcg gccatgctcg ttcgcctggg acctgaggca
     3541 aagccggatc cgcaaagtgc cgagaaggtc gcggcggcaa ccaaggccta cgaggcggca
     3601 aagaaggagt tcgaggcgat ccgcggcacg ccaaaagggc tggccttggc ggccaacggc
     3661 cagccgacgc agaggccgtt tcgattgaag atgatcaaac ttcaaaacga gctgctcgcg
     3721 ctcaccgacc cggccaacgg caaggtggcc tttggggtgc gcgacgccaa gacggtcgct
     3781 gacaccgaga tccgcgtgcg cggggaggcc gagaagctgg gaccagtcgt tccgcgcgga
     3841 tttctctcga ttgtgccgat tgccgatgtg cccaagatca actcgagcca gagtggacgt
     3901 ctcgagctgg cgcagtggat cacgagcgcg aagaatccgc tcacgccgcg cgtgatggcc
     3961 aatcgggttt ggcagcatct cttcggtaag gggttggtcc agagcgtcga caacttcggc
     4021 gtgaccggcg acgtgccgtc gcatcccgaa ctactcgatt acctggcggc aagcttcgtt
     4081 cgcgaggatt ggtcgatcaa gaagctggtg cggaaggtcg tgttgagccg gacctatgcg
     4141 ctcgcgtcgg acgtgtcgga gtccaatttc gctgtcgacc ccgcgaacag gctgatctgg
     4201 cggcatagcc cgcggcggct cagcgcggag gagattcgcg atgccacgct cgcagcggcc
     4261 ggcaagctcg acctgggccg gcccgacggt tcgcccgcca aagatctgaa ggttacggag
     4321 atggcgaaca acggtcccct ggcgcggcga atcggcgaac aagcaagtag gagcacgctc
     4381 cggagcattt atctgccact cttaaggggt gtaacgccga cgtcgctcga tgtcttcgac
     4441 ttcgccgagc aaggcatggt gaccggcagc cgagatacga ccacggtcgc tacccaggcc
     4501 ctttatctgc tgaacgatcc ctttgtacgg cgtcaatctc tggccctggc cgagcgactg
     4561 ctcgatcaaa ccgaggcgga ctgtgccaac cgcatcgacc gggcctaccg gctgacaatt
     4621 tgcaggccgg cgtccgcggc cgagatcgag cgagcgagca ggtacctcgc gagctacgag
     4681 agctccgtgc gtgatttggt cgcgtttact cccccggcgc ccgctctgcg gccgaacact
     4741 gttgttgcgg cggttgacgc cgataccagc gccaaggctg ccgccaagcg gcccgcgccg
     4801 cccgtgaatc ccgacgaggt cgagcaggtg gaagcaccgg tcgcggaaga agtcatcgcg
     4861 gcggccaatc cagagaccgc tgcctgggcc agcttttgcc aggcgctcct ggggagtgcg
     4921 gaatttcgtt atatcaagta agacaagtta ccagaaatct tcataaaatt atctgatagt
     4981 cagcgagttg agagggtgac acatgccgtt cacgcaatcc aagacgacga ggacagggac
     5041 cgaggtttcc cgccggcagg ttctcaagag cgccggcacg ggtttcggtt acctggcctt
     5101 ggctggaatg ctcgggcaga acgtggctaa ggcgcgtgac gggacagcca gccacgcgct
     5161 cgag
//
LOCUS       DATAQL010001026         6942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_255348, whole genome shotgun sequence.
ACCESSION   DATAQL010001026 DATAQL010000000
VERSION     DATAQL010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255348"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VHS97_17740"
     CDS             complement(<1..731)
                     /locus_tag="VHS97_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450101.1"
                     /translation="MDVRRHLIALFMGAATVVVLGTARGQAPATAPKAFADLPSQVFA
                     GAAPVKGQPGAPDPAEAKKQEHLQKIKQLTFDRRPSSILKAWSTPREEALTRPESDDS
                     APGGGPAVVPNMRRMARGVVVATPNGVQATAPSVDAKLDPFDRELKSFQYDVTLGDWS
                     AVKGFLSKLNHDEGKAAFDQLIQSLSNPMGMQGNQQMQMQMQMQMQMQMNMGMQFGMS
                     SPGAPPPQQFMFEKNVFTNQDIFGLA"
     gene            complement(768..1487)
                     /locus_tag="VHS97_17745"
     CDS             complement(768..1487)
                     /locus_tag="VHS97_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450102.1"
                     /translation="MRTLVLLGWLLVPVVVGAYHYGPGQEKLRLDDVARVLADADRLA
                     TQQDWQAASTKYEQALALLPAGRVADARRIRLQRAKVQMFGHQLPEANAALRDLVDQI
                     EDDKQADPEVRDQARDALANAQYYMTWLMRLEGLGAQEWEPEIEGARQTYRMLAEQDE
                     AKGDQESARKHREDLESVIRLARMDLGDLQGLDLPKQCQNCKSGQCKKPGKKPGNKPK
                     GEEKKDARKAGAGPPPDNSGS"
     gene            complement(1603..2625)
                     /locus_tag="VHS97_17750"
     CDS             complement(1603..2625)
                     /locus_tag="VHS97_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX3450103.1"
                     /translation="MVSVSHAELFAAAVAILAAAAESLHSRRCHRLARLAFGPNGRPA
                     LWARSAPALRVGAVAALSWGLVTLVELPPKVHVAESIADKDRRHVLIVLDVSPSMRLK
                     DAGPNAQESRMKRAAVVMDSFLQRVPVEIYLLSVVACYNGAKPVVVDTKDLEVIRNIF
                     SDLPLHHAFAAGKTDLFSGLTAAAKIAHPWQPRSSLLLMLSDGYTVPGTGMPKMPASI
                     ADVLIVGVGDPRQGSFIDGRMSHQDTSTLRQIAARLGGIYHDGNQKHISTDLLNRLTV
                     IPRKSTFERLTRREYALIACGLGSLALAFLPVLLHRFGTRWRPGVRRTVARSSNEALV
                     ASARSY"
     gene            complement(2619..3533)
                     /locus_tag="VHS97_17755"
     CDS             complement(2619..3533)
                     /locus_tag="VHS97_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX3450104.1"
                     /translation="MSFAYPAVLVLLVVPVLLLAWIWKRSAGRIALPFDHGGHDSRWA
                     WAFLLGLAESLPALILATVIVLLAGPQQLSAPKTKRVLTNIEFCVDVSSSMITPLGDG
                     TRYDASMKAIDHFLDIRKGDAFGLTFFGNNVLHWVPLTGDPSAIRCAPPFMKPENVPQ
                     WMGGTMIGKALLACKEILAEREEGDRMIILVSDGESYDLSGGNDESVAQTLKRNNIAV
                     YAIHISDQNPPAEIVNITTLTGGEVFPVDDPGALRAVFERIDAMRETKLEKTASELLD
                     DFVPACMVGLSLIGLATCAFFGLRYTPW"
     gene            complement(3530..4402)
                     /locus_tag="VHS97_17760"
     CDS             complement(3530..4402)
                     /locus_tag="VHS97_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450105.1"
                     /translation="MRFTTATLLIFAHVSVETSLPGAETPRSTSTVGMPARLEQLVLP
                     GTELEAKPIEDRRTPVILRIVNSYPHGSAFRYDIVYYGLEPGEFDLKDSVRRKDGSPI
                     TNLPSISIKIEPVLPPGQIEPHRLSLAPSPFLGGYRLLLIAGGLIWAAGLAAILLAGR
                     RRRGQAVINAIKPLTLADRLRPLVAKALAGTLNQGQHAELERLLIGYWRKRLKLERCA
                     PAEFIPLLRNHEEAGPLLRSLEDWLHRPAGTAEPVDLSALLEPYQAFAADSLEEVQSE
                     ILVSGSSALREGRA"
     gene            complement(4402..5262)
                     /locus_tag="VHS97_17765"
     CDS             complement(4402..5262)
                     /locus_tag="VHS97_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3450106.1"
                     /translation="MEGRIEHHDVLSARQFFLAVKKLADSLSYGMDRSPYLGSGVEYV
                     QSRPYQYGDPIKLIDWRVTARTRKVHVKEYEAPKRMPVYLLVDTSASMTISSHHPSKY
                     ETAVFLAGGLALACLDRISPVGVLGVGGQGLHVRPSMSRDQVLEWLHRLRRFRYDETT
                     SLGARLIELSPSLSHRVLVIVLSDMHDPRAIPALKRLAQEHDCAVLQLRDPAEDRLRG
                     VGFVRATEAETGRALLVRGRVRGSDSAHVGEQLKKAGIDYLLIQTDQPYVSRVRQFFK
                     LRNLLGRGAR"
     gene            complement(5296..6351)
                     /locus_tag="VHS97_17770"
     CDS             complement(5296..6351)
                     /locus_tag="VHS97_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3450107.1"
                     /translation="MSTLETQAHKPGTQPIPRETLEFIRAVRERVGTVVVGQDVVVER
                     LLIALVTGGHLLLQGVPGLAKTLLVAAVSKTIDLKFARIQFTVDLLPSDIIGSEILDQ
                     KSNDFRVHKGPIFTNLLLADEINRAAPKVQSALLEAMQERRVTIGNETFKLPAPFLVI
                     ATQNPIEQSGTFELPEAQLDRFMLCHRLAYPRTDEEKEILRRNAALGVRREDRGAVAK
                     TEFDVLEGGPVAATEDLVRAMEAVHDVYVSETFTDHVVEIVTRTRNHPRIELGASPRA
                     GIALIKGARARALIHGRNYVIPEDLFALAEDVILHRIRLNYEALADGVTGADVLGEII
                     GPYGAPAPSTNGYRKRS"
     gene            complement(6757..>6942)
                     /locus_tag="VHS97_17775"
     CDS             complement(6757..>6942)
                     /locus_tag="VHS97_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450108.1"
                     /translation="VISLAMAVSGVKNLLGPEARTLKATLAFFRRAYSTCGGDRLFSC
                     HLHQRDGAAHDERSHRG"
BASE COUNT         1360 a         2146 c         2116 g         1320 t
ORIGIN      
        1 gccagaccga aaatatcctg atttgtgaag acgtttttct cgaacataaa ctgctggggc
       61 gggggcgccc ccggcgagct catgccgaat tgcattccca tgttcatttg catctgcatt
      121 tgcatttgca tctgcatttg catctgctgg ttgccttgca tccccatcgg attgctcagg
      181 gactggatca attggtcaaa cgcggctttt ccctcgtcat gattgagctt ggacaaaaag
      241 cccttgacgg cagaccaatc gccgagcgtg acgtcgtact gaaatgactt cagctctcga
      301 tcgaagggat caagcttggc atcgacgctc ggcgccgtcg cctgcacgcc attcggggtg
      361 gcgaccacca cgccccgcgc catccgccgc atgttcggca cgactgcagg cccgccaccg
      421 ggcgcggaat cgtcgctttc cggtcgcgtc aaggcctctt cgcgcggagt cgaccacgcc
      481 ttcaatatag acgagggacg gcgatcgaag gtcagttgct tgatcttttg cagatgctct
      541 tgcttcttgg cctcggctgg atcaggcgca cccggctgac ccttgacggg cgcagcgccc
      601 gcaaagacct gagatggaag atctgcaaac gctttgggcg ccgtggcggg cgcctgacct
      661 cgagccgtcc ccaaaacgac cacagtggcg gcgcccatga atagcgcgat caagtgacgt
      721 cgaacgtcca tggtggtgca gtcctctgaa aggcgaaaaa gaatcgctca cgagcccgag
      781 ttatccggtg gaggccctgc acccgccttg cgggcgtctt tcttttcctc tcccttgggc
      841 ttattgccgg gctttttccc gggcttcttg cactggccgc tcttgcaatt ctggcactgc
      901 ttgggaagat cgagaccttg gaggtcgccg agatccatcc tagccaggcg aatcacgctc
      961 tcgagatcct cgcgatgctt gcgcgccgac tcctggtccc ccttggcttc atcctgttcg
     1021 gcgagcatcc ggtaggtctg gcgggcacct tcaatctccg gttcccattc ctgtgccccc
     1081 agcccctcca gccgcattag ccaggtcatg tagtactggg catttgccag cgcgtctcgc
     1141 gcctggtcgc ggacctctgg atcggcttgc ttgtcgtcct cgatctggtc gaccagatct
     1201 cgcagcgcgg cgttggcctc agggagttga tggccgaaca tctggacctt cgctcgctgt
     1261 agccgaatac gacgcgcgtc ggcaacgcgt cccgcgggca gaagcgccaa ggcttgctcg
     1321 tacttagtcg aggcggcttg ccaatcctgc tgggtcgcca ggcggtcggc atcggccaga
     1381 acgcgcgcca cgtcgtccag gcgtagcttt tcctggccgg ggccgtagtg atacgcgccc
     1441 acaacaaccg ggaccaataa ccaaccgagg agcacgagag tacgcatcga tccacctttc
     1501 acgggtcaga aacgtaaacg agacttcggt tccccgtacg atcgcgaaag aaggcccatt
     1561 caatctactt gatcccacga gatcacgata ccttgaagtc catcaatacg accgagccga
     1621 cgccacgagt gcctcgttgg acgaacgtgc cacagttcgg cggacgccgg gcctccaccg
     1681 agttccaaat cggtgcaaga gcacgggcag gaatgccagc gccaacgagc ccagaccgca
     1741 cgcgatcaag gcgtattctc tgcgcgtaag ccgctcgaat gtgctcttgc gcggaatcac
     1801 cgtcaaccga ttgagaaggt cggtgctgat atgcttctga ttgccgtcgt gatagatgcc
     1861 accgagccgc gcggcgatct ggcggagggt cgaggtatct tgatgcgaca tacggccgtc
     1921 gatgaatgag ccctgccggg gatcgccgac tcccacgatc aagacgtcgg cgatcgacgc
     1981 cggcatcttg ggcataccgg tgcccggaac ggtatagccg tctgagagca tgagcaagag
     2041 cgaactcctg ggctgccagg ggtgcgcgat cttggcggct gccgtcagac ctgaaaacag
     2101 gtcggtctta cccgcagcga aggcatggtg gagcggcagg tcgctaaaga tgttacggat
     2161 gacttcaaga tccttggtat cgacgacgac gggcttggcg ccgttgtagc aggccacgac
     2221 agagaggagg tagatctcga ccgggacacg ctgcaggaac gaatccatga cgaccgcggc
     2281 gcgcttcatc cggctctctt gcgcgttcgg tccggcatcc tttaaacgca tgctcggcga
     2341 gacgtcgagc acgatcagga catggcgcct gtctttatcg gcgatggatt cggcgacgtg
     2401 gactttggga ggtaactcga cgagcgtcac gagcccccaa gagagcgcgg ccacggcccc
     2461 aacccgtagc gccggggcag agcgcgccca gagagctggc cgtccgttgg gcccaaacgc
     2521 cagccgggcc aggcggtggc agcgccggga gtgcaggctc tcggcggcgg ccgccaggat
     2581 ggctacagcc gcggcgaaca gctcggcgtg cgagacgctc accatggcgt gtacctcaac
     2641 ccaaagaatg cgcaggtcgc gagaccgatg agcgatagac cgaccatgca cgccggaacg
     2701 aagtcgtcca gtaactcgga ggcagtcttc tccagcttgg tttcgcgcat ggcgtcgatc
     2761 cgttcgaaaa ccgcccgaag agcgcccgga tcgtcaaccg ggaatacctc gccgccggtc
     2821 agcgtcgtga tgttgacgat ctccgccgga ggattctggt ccgagatgtg aatggcatag
     2881 accgcgatgt tgtttcgttt cagagtctgg gcgaccgact cgtcattccc gccgctcaaa
     2941 tcgtagcttt cgccgtcgga aaccaggatg atcatccggt ctccctcctc acgctcagcc
     3001 aggatctcct tgcaggccag gagcgccttg ccgatcatcg tcccccccat ccactggggt
     3061 acattttccg gcttcatgaa tggcggcgcg caacggatcg ccgagggatc gccggtcaag
     3121 gggacccagt gcagcacgtt attgccgaag aaggtcaggc cgaacgcatc ccccttgcga
     3181 atatcgagga agtggtcgat cgctttcatc gaagcgtcat agcgcgtgcc gtctcccagc
     3241 ggagtgatca tgctgctcga aacgtcgacg cagaactcga tattggtcag gactcgcttg
     3301 gtcttgggcg cgcttaattg ctgaggcccc gccagcagca cgataaccgt cgccaggatc
     3361 aaggccggca aagactcggc cagaccgagc aagaacgccc acgcccacct cgaatcgtga
     3421 ccgccgtggt cgaaaggcaa ggcgatccgt cccgccgatc gtttccagat ccaggccaga
     3481 agcagcaccg gaacgaccaa caacacgagc acggcgggat acgcgaagct catgcgcgac
     3541 cctccctaag agcagacgag ccggagacga ggatctcgct ctggacttct tccagggaat
     3601 cggccgcgaa agcttgatac ggttcgagca gcgcgctcaa gtcgacgggc tctgccgtac
     3661 cggcaggccg gtgcagccaa tcttccaggc tccggagtag cgggccggcc tcttcatgat
     3721 tgcggagcaa ggggatgaac tcggccgggg cgcaccgttc cagcttgagc cgcttgcgcc
     3781 agtagccgat cagcaggcgc tcgagctcgg catgttgccc ttgattgagc gtgcctgcaa
     3841 gcgctttggc gaccagcggc cgaagtcggt cggccaaagt caggggcttg atcgcgttta
     3901 ttaccgcctg gcctcgtcta cggcggccgg caagcaagat cgcggccaat ccggcagccc
     3961 agataagtcc gcccgcaatg agcaacaggc gataaccacc cagaaacggc gacggagcca
     4021 gcgacagccg atggggctcg atctgccccg gaggcaatac gggctcgatc ttgatggata
     4081 tcgatgggag attggtgatc ggcgagccat ccttgcggcg tacgctatct ttcaagtcga
     4141 attcgcccgg ctccagccca taatagacaa tgtcgtatcg gaaagccgac ccgtgaggat
     4201 aggagttcac gatccgcagg attaccggcg tgcggcgatc ctcgatcggt tttgcctcca
     4261 gctccgtgcc cggcaacacc agttgctcga gcctggccgg catgccaacg gtgctcgtgg
     4321 accgtggcgt ttcggcaccc gggagtgacg tctcgaccga cacatgtgca aagatgagca
     4381 gagtcgccgt tgtgaaccgc actaacgcgc tcctcggcca agcaagttgc gaagtttgaa
     4441 gaactgccgg actctggaaa cgtacggctg atcggtctga atcaagagat agtcgatgcc
     4501 cgcctttttg agctgttccc cgacgtgcgc gctatcggag ccgcggaccc ggccgcggac
     4561 caagagggcg cggccggtct ctgcctcggt cgctcgaacg aatccaacgc cacgaagccg
     4621 gtcctcggcg gggtcgcgca actggagcac ggcgcaatcg tgctcctggg ccagtcgctt
     4681 caatgccggg atggcccggg gatcgtgcat gtcactcaag acgatcacga ggacgcggtg
     4741 cgagagactg ggcgaaagct cgatcaatcg cgccccgaga gaagttgtct cgtcgtaacg
     4801 aaacctgcgc aatcggtgca gccactcgag aacctgatct cgagacatgc tggggcggac
     4861 gtgcaggccc tggcccccca cgcccaacac gccgaccggg ctgatccggt cgagacaggc
     4921 cagtgccagc ccacccgcca ggaatacggc ggtctcgtac ttgctcgggt gatgcgagct
     4981 gatcgtcatt gatgccgagg tgtcgaccag caagtagacc ggcatccgct tgggcgcctc
     5041 gtattccttt acatgcacct tgcgagtgcg ggcagtgacc cgccagtcga tgagcttgat
     5101 cgggtcgcca tactggtatg gacgcgactg aacatattcg acccccgagc ccaaatacgg
     5161 cgagcgatcc atgccgtagc tcagactgtc ggcgagtttt ttgacggcca ggaaaaactg
     5221 ccgcgcgctc aacacatcgt gatgttcgat ccgaccttcc acaaatcctc cagcgaacgc
     5281 aaagggcggg gggtcttagc tccgcttcct atatccattg gtactgggcg cgggggcacc
     5341 gtagggaccg atgatttcac cgagcacatc cgcgccggtc acaccgtcgg caagagcctc
     5401 gtagttcaag cggatccggt gcaagatcac atcctcggcc agtgcgaaca agtcttcggg
     5461 aatcacataa ttgcgaccgt ggatcaaggc cctggcccga gcgcccttga tgagtgcgat
     5521 gccagcccgc gggctggccc ccagctcgat ccggggatga ttccgtgtgc gggtgacgat
     5581 ctcgaccaca tgatccgtga atgtttcgct cacgtaaacg tcatggactg cctccatggc
     5641 gcggaccaga tcttccgtcg cggcgaccgg ccccccttcg agcacgtcaa actcggtttt
     5701 ggcaaccgca ccgcggtcct cgcgacggac gccgagtgcg gcgttgcgcc gcagaatctc
     5761 tttctcttcg tccgtgcgcg gatacgccag gcgatggcaa agcatgaacc ggtcgagctg
     5821 agcttccggc agctcgaagg tgccgctttg ctcgatcggg ttttgcgtgg cgatcaccag
     5881 aaacggcgcg ggcagcttga aggtctcgtt gccgatcgtc acacgacgtt cctgcatggc
     5941 ttcgagcaag gcactctgca ccttgggcgc agcccggttg atttcgtcag ccagaagaag
     6001 attggtgaag atcggccctt tgtgcacgcg aaagtcgtta gacttctggt caaggatctc
     6061 cgagccgatg atgtcggagg ggagcaaatc gacggtgaac tgaatccggg cgaacttgag
     6121 gtcaatcgtc ttggacaccg ccgcgaccaa aagtgtcttg gccagtcccg gtacaccttg
     6181 cagcagcaga tgtccgccgg tcacaagcgc gatcaacagg cgctcgacaa cgacatcctg
     6241 accgaccaca accgttccta ctcgctcgcg cacggctcgg ataaactcga gcgtctcacg
     6301 ggggatcggc tgagtaccgg gtttgtgggc ctgtgtttcg agggtgctca tgattgccgt
     6361 ggctcactgg ggactgacgg agatgacacc gaacacgtca cgcaagccgt ggcgtgcttg
     6421 accagccacg atggccggct aactcgccgc gcgaagggaa ttctgcgcat cgctcgtctg
     6481 caatgataaa tgacatgaga gcggtccagg caagtctttt ctccgaataa tgtcagagga
     6541 cgactgtctt ggtgtttccg ctcggattgc gcgggtgcga caacggagtc tcgcaggacg
     6601 acctttctct cataggcgag cctgggcgag ccgctcgtcg acccgtctcg ccctcccatc
     6661 gtaaaacgtc gctcttggcc ccgcgcgggg tacatctccc aaacgtcgct accaccagaa
     6721 ggcgtggcaa gcgtgggttc gttcgtcacg ttttcgtcat ccgcgatggc ttcgttcgtc
     6781 gtgtgctgct ccgtcgcgtt gatgtagatg gcatgagaag aggcggtctc ccccgcaggt
     6841 cgagtaagct cgcctgaaga aggccagtgt ggctttcaag gttctggcct cggggccgag
     6901 aaggtttttt acaccggaga ccgccatggc caaggatatc ac
//
LOCUS       DATAQL010001027         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5446495, whole genome shotgun sequence.
ACCESSION   DATAQL010001027 DATAQL010000000
VERSION     DATAQL010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5446495"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(933..>2330)
                     /locus_tag="VHS97_17780"
     CDS             complement(933..>2330)
                     /locus_tag="VHS97_17780"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3450109.1"
                     /translation="ELTLPEAAELGCRACASPGGGCQFLGTAATSQVVAEALGITLPH
                     AALAPSGQAIWRDMARRSARALVAMAARNFTLADVLTQSAIKNAMVVHAAFGGSTNLL
                     LHIPAVAFAAGLRRPTVEDWHEVNVKVPRLVSVLPNGPYYHPTVRAFLAGGVPEVMLH
                     LRGLSLLDLSVVTVTGEPLERVLEWWETSERRKQLRERLLEQDGVDPDDVIMSPQRAS
                     KHGLTSTVTFPRGNLAPIGSVIKSTAIDPTVVDPDGVYRKTGPARVFTRESDAIAAIK
                     GQGEKPIRPGDILVLIGRGPAGAGMEEIYQITAALRYLSFGKHVAVLTDARFSGVSTG
                     ACIGHVSPEALAGGPIGKLRDGDVIRITVDRVNLEGSLDLVGADGVAFDTDEGTRVLA
                     ERSAHPQLSPDPDLPDDTRLWAALQSVGGGTWGGCVYDTDAIVAALNERLRFPATLRE
                     PNEVSLSGQPSGSKT"
BASE COUNT          429 a          699 c          736 g          466 t
ORIGIN      
        1 cctgcaatct cgggcccttt tgtcgccaag gatgatactc agcctatcta cacaatgcct
       61 gagaattgga tcgcacctgg gccagaactg gcacggctgc ctcagtctcc cggatagtct
      121 tcggaactcc gcgctgctcg aggagatagc ggtgcattag gccctttggc acggtatgct
      181 caatgagaac tctggaatcg agaattgaca atgtccatgg cggtgtgacc accgaatcgg
      241 gcggtcagga ggtcgtcgga tgctctttgg ttttcgtggg cattgcctcg attgcggcca
      301 ccagtgggat tggctccggt ccaggatcgc gtgtggacac atcgatttcc acaagcccga
      361 gacatatcga agttactgtt gcccgcgttg cttcgtagag ctggatgtgc cacgtcgact
      421 caatcgcagt tgctggctgc gctgggtctc tgaaaatgcc tccgagctta atcattcgcc
      481 gctacatttc aggacgtgtg aactgggcgt ccgggtcgat cgtcaggcgc ttgaagtgat
      541 tgcgcggtcg cctctgttat tcaaggcctg cgagtgcgtc gcctccatct tggccgggcc
      601 gcgatctcca tacgaattcg tctcgattga catcgggccg atggaatgcc cgcaatgcgg
      661 cgacggcatg gcaattgggg aattcgacac caacccgctg gtgtgcccag aatgtgagag
      721 cccgtctaca agatcgatca cgggacatgc ccatccgacg tccctggtgg attattggcc
      781 cctcgacggc gaagatattc gccgggttat ccgccatctc aaggaaatgg ccgagaactc
      841 cataggctct catttcaagg gaaggtttgc tgtcccggtt tcggaaggac gggctcctct
      901 ctgggaccga gacttggacg gtttggagta ggtcaggttt tcgaacctga cggctgcccg
      961 gaaaggctga cctcatttgg ttctctgaga gtggccggaa accgcaagcg ttcattcaga
     1021 gcggccacga tagcgtcggt atcatagacg cacccacccc atgtgccgcc accgacagac
     1081 tgtaatgccg cccaaaggcg ggtgtcgtcg gggaggtcgg gatcgggtga aagctgtgga
     1141 tgcgcagatc gctcggccag cacgcgcgtg ccttcgtcgg tatcgaacgc gactccatcg
     1201 gcaccgacca ggtcgagcga gccttccaga ttgactcgat cgacggtgat ccggatcacg
     1261 tcgccgtcgc gcaacttgcc gatcggcccg ccagccaggg cctccgggct gacgtgtccg
     1321 atgcaggcgc ccgtggaaac gccggaaaac cgcgcgtcgg tcaagaccgc cacgtgcttg
     1381 ccaaacgaca aataccgcag tgccgctgtg atctgataga tctcttccat gccggcgccc
     1441 gccggacctc ggccgatcaa taccaagatg tctcccggcc tgatcggttt ctcgccctgg
     1501 cctttgatcg cggcaatggc gtcggactcg cgcgtgaaga ctcgcgccgg cccggttttg
     1561 cgataaactc cgtcggggtc aacgacagtc ggatcgatcg ccgtgctctt gatcactgaa
     1621 ccaataggcg ccaggttgcc gcgcgggaat gtaacggtgc ttgtaaggcc atgcttgctg
     1681 gcccgctggg gactcatgat cacatcgtcc ggatcgacac cgtcttgttc caacaatcgc
     1741 tctcgcagct gctttcgccg ctcgctggtt tcccaccatt cgagcactcg ttcgagcggc
     1801 tctccggtga cggtaacgac cgagaggtcg agcaggctga gtccgcgcaa gtgcagcatg
     1861 acttccggca ctccgccggc gagaaaggcg cgcacggtcg ggtggtagta ggggccgttg
     1921 gggagtacgc tgacaagccg cggaaccttg acattgactt cgtgccagtc ctcgaccgtg
     1981 ggccggcgca ggcccgccgc gaaggcgacg gccggtatgt gcagcagtaa gttggtcgag
     2041 ccgccgaacg cggcgtgcac gaccatggcg ttcttgattg cggactgagt gagtacgtcg
     2101 gcgagggtga aatttcgcgc ggccatcgcg accagcgcgc gtgccgatcg gcgggccatg
     2161 tcgcgccaga tcgcctgacc ggatggggct aatgcggcgt gtgggagcgt gatcccgagc
     2221 gcctcggcga cgacctgtga agtggccgcg gttcccagaa actggcagcc gccaccggga
     2281 ctggcacagg cccggcagcc cagctcggca gcctcgggca aggtgagctc
//
LOCUS       DATAQL010001028         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_562919, whole genome shotgun sequence.
ACCESSION   DATAQL010001028 DATAQL010000000
VERSION     DATAQL010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562919"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VHS97_17785"
     CDS             <1..1605
                     /locus_tag="VHS97_17785"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF002727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX3450110.1"
                     /translation="GVIFESFTASAERAMLRADLLAKRRRGSVVEPLDLLAALASEPE
                     TRASELLVELGVEIDRLWDGLGPELSLPLAALKDDPAFIDETSSAHSLLPVSPALRLV
                     LNEATISARGSDRKRELGTEHLLAGLLSTSGPAADLLQVAGLEIQTLRQRLTEAVEVD
                     VAPLPPAEGIPPLELNEAGGVELGRILDASANRAREGLRVIEDYVRFVLDDPGLTRRL
                     KQVRHRLAEAERGLDDKLLIGSRDTREDVGTHIMTHTERVRETPRAVLAANFKRVAEA
                     LRSLEEYCKLIDVWLAGRYEVLRYDVYTLEKITMTAVAAQRSLGQAKLMVLVGGLPTL
                     GDLTWVVGEALAGGADVIQYREKGLPDRELLRRAREVRILTAQARVPLILNDRVDLAR
                     LASCDGVHVGQEDLSPRDARRIMGATAHIGMSTHDHRQLDSAILSGASYLGVGPVFPS
                     PTKEFSEPELAGLGFIRFASETTTLPWFAIGGINESNVDRVFEAGATRIAVSAAVVRA
                     DSPRRATRRLKARLTGSDDDSQDPHA"
     gene            complement(1615..>2748)
                     /gene="hemL"
                     /locus_tag="VHS97_17790"
     CDS             complement(1615..>2748)
                     /gene="hemL"
                     /locus_tag="VHS97_17790"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX3450111.1"
                     /translation="YIDYIGSWGPMILGHAHPKVRSAVIGALELGSSFGAPTVREIEI
                     AETVCRAVPSIEKVRFVSSGTEAAMSAVRVARGVTGRSKIIKMAGHYHGHIDALLVQA
                     GSAATTLGTPNSPGVTEGAVQDTILCPFNDVKAVSDVLAQFPGQVAAVLLEPIAGNMG
                     LVPPQPGYLEALRELTAKDGSLLIFDEVMTGFRVAYGGAQALYGITPDLTALGKVIGG
                     GLPAAAYGARAPIMNQVSPVGPIYQAGTLSGNPLAMAAGLATLQILRDESPYDRMEAL
                     SARLAEGLDRAATDAGVPHVVQRVGSMLTLFFHDGPVFDYDDARRSDTKLFARFFWEM
                     LARGVYLPCSQFEAAFVSAAHTEGDIDQTIAAAREALEVCRKL"
BASE COUNT          506 a          852 c          895 g          495 t
ORIGIN      
        1 ggagtgatat tcgagtcgtt tacggcgagt gccgagcgag ccatgttgcg agccgatctg
       61 ctggcaaaac gtcgccgtgg gtcagtcgtt gagcctctcg acttgctcgc tgcactcgcg
      121 tccgagccgg agacccgtgc ctcggagttg ctggtggagc tgggcgtcga gattgacaga
      181 ctctgggacg gtctcggtcc ggagttatcg ctgcccttgg cagcgctcaa agacgatcct
      241 gccttcattg acgagacatc gagcgcccac agcctattgc cggtgtcgcc ggccttacgc
      301 ttggtgttga acgaggccac gatatcggcg cgggggtcgg accgaaaacg cgaattgggc
      361 accgagcatc tgctggcggg gttattatcg acgtcggggc cagccgccga tttgctccaa
      421 gtcgccggac tagagattca aactctgcga cagcgattga cagaggcggt cgaagtcgac
      481 gtcgctccac tcccgcctgc tgaggggatt ccgcctctcg agctgaatga ggcgggaggc
      541 gtcgagctgg gacgaattct tgacgcttca gccaaccggg cgcgcgaagg actccgcgtg
      601 atcgaggact atgtccgttt cgtgctcgac gacccggggc tgacccgacg gctcaagcag
      661 gtccgccaca ggctggcgga ggcggaacgt gggctcgacg acaaactctt gatcgggtcg
      721 cgcgataccc gcgaagacgt ggggacgcac atcatgaccc acaccgagcg ggtccgtgaa
      781 accccgcgcg ccgtcctggc ggcgaacttc aagcgagtcg ccgaggccct gcggtctttg
      841 gaggagtact gcaagctgat cgacgtttgg ctcgccggac gctacgaggt cttgcgctac
      901 gacgtttata cgctcgagaa gatcacgatg accgccgtcg ctgctcagcg gagcttgggg
      961 caggcgaagc tcatggtctt ggtcggcgga cttccgacgc tcggcgacct cacgtgggtg
     1021 gtgggcgagg cattggccgg cggcgccgac gtgattcaat atcgtgaaaa aggtcttccg
     1081 gatcgcgaac tgctgcggcg ggcgcgcgag gttcggatcc tgaccgccca ggcccgcgtt
     1141 cccttgatct tgaacgatcg agtcgatctg gctcgactcg cctcttgcga tggggtccac
     1201 gtcggccaag aagacctatc tccgcgcgac gcgcggcgga tcatgggcgc aaccgcgcac
     1261 atcggaatgt cgacccatga ccaccggcag ctcgactccg cgatactctc aggcgcaagt
     1321 tatctcggag tcggccccgt gtttcccagc cccaccaagg aattctcgga gcccgagctc
     1381 gcagggctag gcttcattcg attcgccagc gaaaccacga cactgccgtg gttcgccatt
     1441 ggcgggatca atgaaagcaa cgtcgataga gtcttcgaag ccggcgcgac tcggatcgcc
     1501 gtcagcgcgg cagtggtgcg agccgacagt ccccggcgcg cgacccgacg actcaaggcc
     1561 cgcctgacgg gatctgatga cgattcccag gatccgcacg cgtagctgca gtagtcatag
     1621 cttccggcag acctctaatg cttcgcgggc cgccgcgatc gtctgatcga tgtcaccctc
     1681 ggtatgggcg gccgacacaa aagcggcctc gaactggctg cacggcagat aaacgccgcg
     1741 cgcgagcatc tcccagaaga agcgtgcgaa caacttggtg tcgctccgtc gcgcgtcgtc
     1801 gtaatcaaac accggcccat cgtggaaaaa gagagtcaac atactgccga cgcgctggac
     1861 cacgtggggc acaccggcgt cagtggcggc gcgatcgagg ccttcggcca gtcgggcgga
     1921 aagcgcctcc atccgatcgt agggggactc gtcgcgcaat atttgcaatg tcgccaggcc
     1981 ggcggccatg gccagggggt tcccggaaag tgtacctgcc tggtaaatcg ggccgaccgg
     2041 cgagacttgg ttcatgattg gggcccgtgc cccgtaggcc gctgctggta agccgccacc
     2101 gataaccttg ccaagtgccg tcaggtcggg cgtgattcca tacagggctt gcgcgccgcc
     2161 gtaggcgaca cggaagcctg tcatcacttc gtcgaaaatg agcaaactac catccttggc
     2221 agtcagttcg cggagcgcct cgaggtagcc cggctggggt gggaccaggc ccatgttgcc
     2281 ggcgattggt tcgagcagta ccgcggctac ctggccgggg aattgcgcca gcacatccga
     2341 cacggccttc acgtcgttga atggacaaag gatggtgtct tgcacggccc cttcggtcac
     2401 gcccgggctg ttgggcgtgc cgagcgtggt ggcggccgag cccgcctgga ccaagagggc
     2461 gtcaatgtgc ccatgatagt ggccggccat cttgatgatc ttggatcggc cggtgacgcc
     2521 acgtgccacc cgcactgccg acatggccgc ctctgtcccg gatgaaacga acctcacttt
     2581 ttcgatcgag gggaccgctc ggcagaccgt ttcggcgatc tcgatctccc gcactgtggg
     2641 cgcaccaaag ctcgagccca gctcgagagc cccgatgacc gcggatcgaa ccttgggatg
     2701 ggcatgcccc agaatcattg ggccccaaga gccgatgtag tcgatgta
//
LOCUS       DATAQL010001029         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2088563, whole genome shotgun sequence.
ACCESSION   DATAQL010001029 DATAQL010000000
VERSION     DATAQL010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088563"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1610
                     /gene="glgA"
                     /locus_tag="VHS97_17795"
     CDS             120..1610
                     /gene="glgA"
                     /locus_tag="VHS97_17795"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX3450112.1"
                     /translation="MNVVFVASEAVPFAKTGGLADVAGALPRALEKQGHSTVLFMPCY
                     RSAWSAGPELIDTGKNIRIPIGARWVEGQVYESRLPRSNVLVYLIGQSHYFDRDGVYG
                     GVDGNDYTDNCERFVFFNRAVLETITALELRPDVIHCNDWQTGLIPVYLKTIYQRVPE
                     LASIGTLHTIHNLAYLGLFWHWDMALTGLGWELFNWQQLEFHGKLCFMKAGLVFADML
                     STVSPTYAQEIQTPGLGSGLEALLRFRQADLRGIVNGIDAEAWNPRNEPMIAARYDAD
                     TVAARKPECKAWLQRKAGLVEHPSIPLFAQIGRLDPQKGWDLLAEVAEPLLERGAQLV
                     ILGEGHLKYRILLEDLARRHAGKFWAYLGFSADLAHQIEAGADIFLMPSLFEPCGLNQ
                     LYSLAHGTVPVVRATGGLADTVIDANSRTLADGTATGFVFDDATPQALWSTIERVLAL
                     WPDRDNWLKLMKTGMNTDWSWERSAREYIRLYEQIKIRVESRPART"
     gene            1638..>3714
                     /locus_tag="VHS97_17800"
     CDS             1638..>3714
                     /locus_tag="VHS97_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEX3450113.1"
                     /translation="MSQVRLILVLHNHQPVGNFDGVFEEAFRKSYRPFLDVLENYPEI
                     TFVLHTSGPLLEWLVEKHPEYIARLKGLVASGRVEILGGAFYEPILTMIPQRDRIGQI
                     QAFSTYIKEILDARVRGMWVAERVWEQHSVSAIADAGIEYTVLDDFHFELAGCKGNDV
                     FGYYLTEDDGRLLKVFPAAEKLRYSMPFQEPHATYEFLKQLAASRPGSTVVFADDGEK
                     FGSWPKTFDHVYTNGWLKHFCDMLRANRDWIETTTLARVADNTLPLGKLYLPDGAYRE
                     MTEWALEPESLRAFRDGAERLGRQTDTGDIKRFFRAGGYWRNFKTRYPESDEMYVRMM
                     VVSRRLAVVQANPSADPDHLEVARQELYRAQCNCPYWHGSFGGLYLPHLRNAIYRGLI
                     GSHNALDEAEGRSGPRISLEVGDFNLDARQEVCLESDRLIVWVRPALGGHIYELDVRE
                     NAINLLATLDRRPEVYHAAIVAAAAKRAHGDNNSGQGRGGETTPGRITIKQEGLENRL
                     VYDRYPRKALVDHFYPADVTLDDLIACREAELGDFVLGTYLAKAQRDVDRVAVVMERP
                     GLAAGHSIRVRKSIELVAGKSGLSVHYELDELPAGVVLHFAVEINVAAMAGHAPDRYY
                     SDETGTKLGLLDDRLDVPHIRDLALTDQWLDLRVTLAWSKSAGLWCFPIETVSQSDGG
                     VEGVYQSSAI"
BASE COUNT          717 a         1080 c         1144 g          773 t
ORIGIN      
        1 ccggcgagga tggtcgattg ggattcggag caggtcgcgc acgcttacgc cgaggcttgc
       61 cgcagggtgc gggcgatcca gactacgcgt cacggcgccc aagccgtgtt gacacacgga
      121 tgaacgtggt ttttgtcgcc tcggaagccg tgcctttcgc caagacgggg ggcctggctg
      181 acgttgccgg cgccttgccg cgcgcccttg agaaacaagg ccattccacg gtcttgttca
      241 tgccttgtta ccggagcgcg tggtcggcgg gaccggagct gatcgacacg ggtaagaaca
      301 ttcgaatccc gattggcgcc cgctgggtcg agggtcaggt ctacgagagt cggcttccgc
      361 gctccaacgt gttggtttat ctgatcggcc aatctcacta cttcgaccgc gacggcgtct
      421 atgggggagt cgatggcaac gattacactg acaattgcga acgctttgtt tttttcaatc
      481 gcgccgtgct ggaaacaatc acagccctgg aactgcggcc cgatgtcatc cactgcaacg
      541 actggcaaac cggattgatt ccggtttatc tgaaaacgat ctatcagcgc gtccccgagc
      601 tggcatcgat aggcacgctg cacacgattc ataatctggc ctacctcggg ttgttttggc
      661 actgggacat ggcgttgacg gggctcggct gggagctctt caattggcag caacttgagt
      721 ttcacggcaa gctctgcttc atgaaggcag gcctggtctt cgccgacatg ctctccacgg
      781 tcagcccaac ctatgcccaa gaaatccaga cgcccgggtt gggatcggga ttggaggcgc
      841 tcttgcgttt ccggcaggcc gacttgcggg ggatcgtcaa cgggattgac gccgaggcct
      901 ggaatccccg caacgagccg atgattgccg ctcggtatga cgccgatacg gtcgccgccc
      961 gaaaacctga atgcaaggcc tggcttcagc gcaaggcggg cttggtcgag catccgagca
     1021 ttcccctctt tgcacagatc ggtcgtctcg acccccaaaa aggctgggat cttctggcag
     1081 aggtggccga gccgctcttg gaacgaggag ctcagctggt gatcttgggg gaaggacatc
     1141 tcaagtaccg cattctgctt gaggacctgg cgcgtcgtca cgccggcaag ttctgggcgt
     1201 atctgggctt ttcggccgac ctggcgcatc agatcgaggc cggcgccgat attttcttga
     1261 tgcctagcct gttcgaaccg tgcggtttga accagctcta cagcctggcg cacggaaccg
     1321 ttccagtcgt gcgggcgaca ggcggcttgg ctgataccgt tatcgacgcc aactcgcgaa
     1381 cccttgcgga tgggaccgct acgggttttg tgttcgacga cgcaacgcca caggctctct
     1441 ggtcgaccat cgaacgggtt ctcgcccttt ggcccgaccg cgacaactgg ctaaaactga
     1501 tgaagaccgg aatgaatacc gactggtcgt gggaacgaag cgctcgagaa tatatccggc
     1561 tctatgagca gattaagatt agagtcgagt cacgtcccgc tcgcacttga gaccgccgtc
     1621 aatttcaagg atcgaaaatg tcgcaggttc gcctcattct agtgctccat aatcaccagc
     1681 ccgtgggtaa tttcgacggg gttttcgagg aggcatttcg taagagttac cgcccgttct
     1741 tggacgtgct cgaaaactat cctgagatca cctttgtcct gcacacctcc ggtccgctcc
     1801 tggaatggct cgtcgaaaag catcctgaat atatcgctcg cctgaaaggc ctggtggcct
     1861 cgggccgagt cgagatcctg gggggagcgt tttacgaacc gatcctgacc atgatcccgc
     1921 agcgggatcg aattggtcag attcaggcat tctccactta cataaaggag atactagacg
     1981 cccgggtacg cgggatgtgg gtggcggagc gggtgtggga acagcactcg gtatcggcaa
     2041 ttgccgatgc tggtatcgag tacacggttc tggacgactt ccacttcgag ctcgccggct
     2101 gcaaaggtaa cgacgtcttc ggctattacc tcacggagga cgacggccgt ctactcaagg
     2161 tttttccggc ggccgagaag cttcgttaca gtatgccttt tcaagagccg catgcgactt
     2221 acgagttctt gaagcagctg gccgcgagcc ggcccggctc aactgtcgtt ttcgccgacg
     2281 acggcgaaaa gttcgggagc tggccgaaaa cgttcgatca cgtttataca aacgggtggc
     2341 tgaaacactt ttgcgacatg cttcgggcca atcgcgactg gatcgagaca acgaccttgg
     2401 cgcgcgtcgc cgataatacg ctgcctctgg gcaaattata cctgccggac ggcgcgtatc
     2461 gtgagatgac cgagtgggcg ctcgagcccg agtcgctacg ggcttttcga gatggtgccg
     2521 agcggctggg ccggcagacc gacaccggcg acatcaagcg gtttttccgc gcgggtggat
     2581 actggcgcaa tttcaagacg cgctatcccg aaagcgacga aatgtacgtg cggatgatgg
     2641 ttgtctcgcg tcgacttgcc gtcgtccagg cgaatccgag tgcggatcca gatcacctgg
     2701 aggtggcgcg ccaagagctt taccgcgcgc agtgcaattg tccctactgg catggttcgt
     2761 tcggcggcct ctatctgccg catttgagga atgcaatcta ccgtggcttg atcggctctc
     2821 acaacgcgct tgatgaagcc gaagggcgct cgggtccgcg tatttctctg gaggtcggcg
     2881 actttaacct cgatgcccgt caagaggtct gcctggagag cgaccggctc atcgtctggg
     2941 tccgcccggc gctcggggga cacatttacg agctggacgt gcgggaaaat gccataaacc
     3001 tgctcgcgac actcgatcgc cgcccggagg tctaccatgc agccatcgtg gctgccgcgg
     3061 ccaagcgcgc ccacggtgac aacaactccg gtcaagggcg cggcggcgag acgacgccgg
     3121 gccggatcac gatcaagcag gaagggctcg aaaatcgact ggtctacgac cgttatcctc
     3181 gcaaggcgct cgtggatcat ttctatccgg ccgatgtcac gctggacgac ttgatcgcgt
     3241 gccgcgaggc cgagcttggt gatttcgtgc tcgggaccta tctcgcgaaa gctcagcgcg
     3301 acgtggaccg tgtcgccgtg gtgatggagc gacccgggct ggcggccggt cattcgatac
     3361 gggtcaggaa gtccatcgag cttgtcgcgg gcaaatcggg cctgtcagtg cattacgagc
     3421 tggacgagct accggccggc gtcgttctcc atttcgccgt cgaaatcaac gtggcggcca
     3481 tggccgggca cgcgcccgac cgatattatt ccgacgaaac tggcaccaag ctcggtctgc
     3541 tcgacgaccg gctggatgtg ccccacatcc gcgacctcgc cctcaccgat cagtggcttg
     3601 acctccgcgt cacactcgcc tggtcgaaat cggccggctt atggtgcttc cccattgaga
     3661 cggtcagcca gagcgacggg ggcgtggagg gagtgtatca atcttcagca atca
//
LOCUS       DATAQL010001030         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2395850, whole genome shotgun sequence.
ACCESSION   DATAQL010001030 DATAQL010000000
VERSION     DATAQL010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395850"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1141)
                     /locus_tag="VHS97_17805"
     CDS             complement(131..1141)
                     /locus_tag="VHS97_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX3450114.1"
                     /translation="MARRVFRTVVGSWLLIGLSAGSVRAAAWTDALFPENRYDFGMVP
                     RGVKVKHDFLLVNRLAEPITILNLRPSCGCTSGRTNTSTVAPGQTAVIEAEMDTRNFV
                     GQKSTVLYVSLITASGRQGEVGLGVISHILSDIVLNPGAITFGTVTRGQSPTQVLTID
                     RINAPGWKFVRMVSTCRAIDANLAETSRTATSASYKLTVSMKPDAPAGILHDEIRLIS
                     NDSEAPSIPLMVTAVVRGDLSAAPSVLPLGQIHSAAGAQGRFVVRSSRPFKIQSIEGV
                     GDGFSTSLPDGTRQPMHVVTVAYKPEEGTTRGDIRHIFRVHTDLPGEPPLDLTATLHV
                     DP"
     gene            1522..>2512
                     /locus_tag="VHS97_17810"
     CDS             1522..>2512
                     /locus_tag="VHS97_17810"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX3450115.1"
                     /translation="MHDVETARPPLPDEAMSLPARYYIDPEYYRFELESFFFKMWVHA
                     GRADEIPNPGDFLVREITGESIILVRNHEGGLSAYYNICRHRGTRLTEEKSGRFAPTI
                     RCPYHAWTYDLKGCLLAAPQMDRVANFRLEEYPLHQVAIDLWDGHIFLHLGEDPVPLT
                     QQLGGMDERFGPWGMGHLKSGARIVYDVAANWKLIIHNYSECLHCPGVHPALQKLSHY
                     LTGENEPATEGVVGGRMRLRDGIATLSLDGQSRRACLHGLPADECRYVYYYAFLPNLL
                     LSPHPDYVMMHTLWPRAVGRTEIVCEWLFHPETMAEPGFDTDDAVAFWDVTNRQ"
BASE COUNT          490 a          782 c          731 g          509 t
ORIGIN      
        1 caagagccaa aaccatccgc tgcatgatcg atctccggga cggtgcgttg ttgtccagca
       61 agttgtattg tatcagcaaa atggctccct gtcccggccc ggcgccagat cgcagcacac
      121 acagtccgga tcacggatcc acatgcaagg tggcagtcaa atcgagggga ggctcaccgg
      181 gcaggtccgt gtgcacgcga aagatgtggc gaatgtcacc gcgagtcgtt ccctcttcag
      241 gcttgtaggc gactgtcacg acgtgcattg gctggcgcgt cccgtcggga agcgacgtcg
      301 aaaacccgtc gccgaccccc tcgatcgact ggatcttgaa agggcgcgat gaacggacga
      361 caaaccggcc ttgcgcaccg gccgccgaat gaatctggcc cagaggcaag accgagggcg
      421 cggctgatag atcaccccgc acgacggccg tcaccatcaa tgggatgctc ggcgcttccg
      481 aatcgttgct gatcagccgg atctcgtcgt gcaagatccc cgccggcgcg tcgggcttca
      541 tgctgaccgt gagcttgtaa ctcgccgacg tcgctgtccg ggaggtctcc gcaaggttgg
      601 cgtcgatcgc ccggcacgtc gacaccatgc gtacaaactt ccaccccggt gcattgatgc
      661 ggtcgatcgt caagacctgg gtcggcgact ggccacgcgt gacggttcca aaggtgatcg
      721 caccggggtt caagacgatg tcgctcagga tgtgggaaat cacgccgagt cccacctctc
      781 cctggcggcc gctcgccgtg atcaggctca cgtaaaggac tgttgacttc tgcccgacga
      841 agttgcgggt gtccatctcg gcttcgatca ccgccgtctg gcccggggcg accgtcgacg
      901 tatttgtccg cccgctcgtg catccacacg agggccgcag gttgaggatt gtgatcggtt
      961 cggccagccg gttgaccagg agaaagtcgt gcttgacctt cacgccgcgg ggaaccatgc
     1021 cgaagtcata tcggttctca gggaaaagtg cgtccgtcca tgcggcggcc cgcaccgatc
     1081 ccgcgctcaa cccgatcaat agccagcttc ccacgacggt ccgaaacacc cgtcgagcca
     1141 tcgccgcctc ctgcggtatc ccgcatcctc tcgtcagcgg ctcgcgcgcc gcccaaatgg
     1201 ccttgttctc ttcctatcgt caagacaaac cccagcgctc agttttggac gatgacgcag
     1261 ctatgccaac acccgttcaa gacttaaact ttcgcgagac cccctatttg cccgtgttgc
     1321 gattgaactt atgacactga acgcaactca tcgtgagcgt gacgaatgcg agcgtggcgg
     1381 cgtcgacagt gttgattcgc ttcgcaaatc cttcgcttgg ctccccatga tcgacggttt
     1441 acgatgtacg gtagacgagt gactcggccg gtatcataag gacgctttgt cctcgatcag
     1501 aattaacccg gatacgaacc gatgcacgat gttgagacgg ctcgaccccc gttgccggac
     1561 gaggcgatga gcctaccggc ccgttattac atcgatccgg agtattatcg cttcgagctc
     1621 gagtcgtttt tcttcaagat gtgggtccac gcgggacgtg ccgacgagat tcccaacccg
     1681 ggcgattttc tcgttcgcga aatcaccggc gagagcatca ttctggtgcg gaaccacgaa
     1741 ggcggcttga gcgcgtatta caatatctgc cgccaccggg gcacccgact gaccgaggaa
     1801 aagtcgggac ggttcgcacc gacaattcgg tgtccctacc acgcctggac ttatgacctg
     1861 aaaggctgtt tgctcgccgc gccgcagatg gaccgcgtcg cgaatttccg gctcgaagag
     1921 tatcctcttc accaagtcgc cattgacctg tgggacggac acatctttct tcatctgggt
     1981 gaagatccgg tcccgctcac gcaacaactc ggcggcatgg acgagcgctt cgggccctgg
     2041 ggaatgggcc acctaaaatc aggcgcccgc atcgtttacg atgttgcggc caattggaag
     2101 ctgatcattc acaattactc ggaatgcctt cactgtccgg gcgtccatcc ggcgctgcaa
     2161 aagctttcgc actacttgac cggtgagaat gagccggcga cagagggtgt tgtcggcggt
     2221 cgcatgcgtt tgcgcgatgg gattgccacg ctgtcgcttg acggacagag ccgccgcgcc
     2281 tgcctgcacg gccttcccgc cgacgagtgc cgctacgttt actactacgc ctttctgccg
     2341 aacttgcttt tgagcccgca ccctgattac gtgatgatgc atacgctatg gccgcgtgcg
     2401 gtaggccgga cggagatcgt ctgcgaatgg ctgttccacc cggaaacgat ggctgaaccg
     2461 ggtttcgaca ctgacgacgc agtcgcgttc tgggacgtga ccaatcgcca ag
//
LOCUS       DATAQL010001031         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_563010, whole genome shotgun sequence.
ACCESSION   DATAQL010001031 DATAQL010000000
VERSION     DATAQL010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563010"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1329)
                     /gene="proB"
                     /locus_tag="VHS97_17815"
     CDS             complement(172..1329)
                     /gene="proB"
                     /locus_tag="VHS97_17815"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX3450116.1"
                     /translation="MQPPTVLARDLVRDEVVLSAQTWVVKVGTSVLTGPDGTLDPARI
                     DHLAEQISAVMDRGRKVALVSSGAVGAGMGQLGWKRRPDNVRQLQAAAAVGQAYLIRA
                     YDEGLRRHRRHAAQLLLTHEDFDSRPRYLNMRNTLHALFECDAVPVINENDTISVDEI
                     KFGDNDRLAAMVTNLLQAPLLVILSVVDGLYRGEPGGSTVPELVSMVPQIDDEVIGLA
                     GLGKSSLGTGGMQSKLAAARLVTRAGGSVIIASGTKPDPLTRILAGEPAGTLFLAHGA
                     THRARKRWIGLTARPRGHYVVDAGARLALESGSKSLLAIGILEVVGEFDKGDVVGIRD
                     ASGQEFARGLTNYSTTDAHRIRGLRTHEARQTLGATLYDEVIHRDNLVLIV"
     gene            1565..>2011
                     /locus_tag="VHS97_17820"
     CDS             1565..>2011
                     /locus_tag="VHS97_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450117.1"
                     /translation="MQPPTIGGAYSTGWTDQMGELHHECGVAAVYHAAGRPVSSLAPI
                     QGDVNSVARLVPRMLLDMQNRGQLAAGMASFRPDRNALINTHKDVGTVAESFRLNHRA
                     EFEAIIREVDGPVAIGHVRYATCGGNDRSDAQPFERDHGRKAKWFAF"
BASE COUNT          351 a          623 c          667 g          370 t
ORIGIN      
        1 ggagcaattg agccgcgtgg cggcgatgtc ggcgcaggcc ttcgtcgtaa gcccggatca
       61 ggtacgcttg gccgacggcg gcggccgcct ggagctgacg caagttgtcg ggccggcgtt
      121 tccagccgag ctggcccacg cccgccccga ccggggcaac accaatgttc gtcacacaat
      181 taggacgagg ttgtcgcggt ggatgacttc gtcataaaga gtcgcgccca aggtctggcg
      241 ggcctcgtgg gtacgcagtc cgcggatgcg atgggcatcg gtggtgctgt aattagtgag
      301 accgcgtgcg aattcttgtc cgctggcatc gcggataccg acgacgtcgc ctttgtcgaa
      361 ctcaccgacc acctcgagga tgccgatggc gagtaagctc ttcgagcccg attcaagagc
      421 cagccgcgcg ccggcgtcga cgacatagtg tccgcgcggc cgcgcggtca agccgatcca
      481 ccgcttgcgt gcgcggtgcg tggcgccgtg agcgaggaag agcgtaccag cgggctcgcc
      541 agcgaggatc cgcgtcaacg ggtcgggctt ggtccccgag gcgatgatca ccgaaccgcc
      601 cgcccgtgtt accagtcggg ccgccgccag cttgctttgc atgccgccgg ttccgagcga
      661 gcttttgccc aggccggcca ggccgatcac ttcgtcgtcg atctgcggca ccatcgagac
      721 cagctcgggc acggtcgaac cccccggctc accgcgataa aggccatcga cgacactcaa
      781 aataaccaga aggggagcct ggagtaggtt ggtaaccatg gcggcgagac gatcgttgtc
      841 gccaaacttg atctcgtcga cactgatcgt gtcgttttca ttgatgacgg ggacggcatc
      901 gcattcgaaa agtgcgtgga gggtgtttcg catattaagg tagcggggcc ggctgtcgaa
      961 gtcttcatga gtgaggagca attgagccgc gtggcggcga tgtcggcgca ggccttcgtc
     1021 gtaagcccgg atcaggtacg cttggccgac ggcggcggcc gcctggagct ggcgcacgtt
     1081 gtcgggccgg cgtttccagc cgagctggcc catgcccgcg ccgaccgctc cagagctcac
     1141 gagcgccacc ttgcgaccgc gatccatgac ggcgctgatt tgttcggcga gatgatcgat
     1201 ccgggctggg tcgagcgtcc cgtccggacc ggtgagaacg ctggtcccca ccttgacgac
     1261 ccaggtttga gcggacagaa cgacctcgtc gcgcacaaga tcgcgcgcaa gcaccgttgg
     1321 cggctgcatt tggacccttg gatccgtcaa gctgggtttg tctggcgtgt ggttcttgtt
     1381 cagtgtcaaa tgcggtgacc ccgacctaca attaccgaag ggcgagagta gcctgatgcg
     1441 gcgggcactc aggtcgcgcg atcgtctttc gatcgtgaag gttgtcggta gatgtcttcg
     1501 acattataat gcccgctcac ggctggccta gagaatgagt ctggggccgc cttgggatcg
     1561 gaccttgcaa ccccccacca tcggtggggc ctactccacg gggtggaccg accagatggg
     1621 tgaactgcac catgagtgcg gcgtggcagc cgtttaccac gccgcggggc gcccagtatc
     1681 ttcactcgcc ccgatccagg gcgacgtcaa cagcgtggca cggctagtgc cgcggatgtt
     1741 gctcgacatg caaaatcggg gtcagctcgc cgccggcatg gccagcttcc gccccgaccg
     1801 caatgccctc atcaataccc acaaagatgt tggcacggtc gccgagtcat tccgcctcaa
     1861 tcaccgcgct gagttcgagg cgatcatccg cgaggttgac gggccggttg cgatcgggca
     1921 cgtgcgttac gccacctgcg ggggcaacga ccgatccgac gctcagccgt ttgagcgcga
     1981 ccatggccgg aaggcgaagt ggtttgcctt c
//
LOCUS       DATAQL010001032         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3005279, whole genome shotgun sequence.
ACCESSION   DATAQL010001032 DATAQL010000000
VERSION     DATAQL010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005279"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1742
                     /locus_tag="VHS97_17825"
     CDS             111..1742
                     /locus_tag="VHS97_17825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3450118.1"
                     /translation="MAQLQTLLSRTLMVATFRSATGRLGCPGLAQATLIVVVAGLLWR
                     TVRYALAFPLWGDEAFVAVNFLARNLVGLTRPLEYFQIAPPGFLLAEWLAIHVFGTGE
                     RALRLVPYLAGVASLLLFWRFCRGVASRRTVLLAVAMLAASFFPVRHSTEVKPYAIDL
                     LVSLMLISTGWAVGRDLRSHRAWLALFGVTIVGVWCSYAAVFPAAGVALFLGARVVRE
                     RSSRLVALWLTHGLLMTLSWGIMFVTFAHPQARAADFLPLLETWRDAFPPLAEPLRLP
                     FWLLEIHTGYMLAYPYGGNDFGSALTTLLVVAGGIRMARRRARRPLLFLLLAPLPVAL
                     VAAALHRYPYGTSTRVMLYMAPAFCLLTGEGIMSLLQLRQWSSRGPLIGAGLLALIPL
                     VCTAFNVASPYKAYDDVLHRNLARWVATRTAPGDQWVVFNGASPPPPVKDLMVMPWLQ
                     RVGEAHFYLLKYAPVPLHWEPAPETVVPNIGGKIWLIIQNYGDADYFPEGRLLAYQGA
                     FEERFGRPRKTTRFTLPRGESWSICEYSPACRAAP"
     gene            1936..>2189
                     /locus_tag="VHS97_17830"
     CDS             1936..>2189
                     /locus_tag="VHS97_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX3450119.1"
                     /translation="MAVLLVPVCLRTFASNPADDRPISPELSRKTAISPLERRFGDTV
                     RPFVDSYCVGCHGKDKPKGDLDLTVYTSADAVTKDLGQWEV"
BASE COUNT          336 a          679 c          690 g          484 t
ORIGIN      
        1 agctgaattc ggacgcataa cccgctgttg tcgcgcgcaa tgcatatttc tcggcgccgg
       61 tgaaccggcg tagttgaatt gaatcagacg ggaaaatgga ggttccgagt ttggcgcaac
      121 tccagaccct gctttcccgg acgttaatgg tcgccacttt ccggtcggcg acagggcgat
      181 tgggctgccc cggcttggct caggccacgc tgatcgtggt cgtcgctggg ctcctctggc
      241 ggacggtacg ctatgcgtta gcctttccgc tctgggggga tgaggcgttc gtcgccgtaa
      301 acttcctcgc gcgaaacctc gtgggactga caagaccact ggagtacttc cagatcgcgc
      361 cgcccggatt cctgctggcc gaatggctcg cgatccacgt ctttggcacg ggtgaacggg
      421 cgttgcgatt ggtcccttat ctggcaggcg tggcgtcgct gttgttgttc tggcgattct
      481 gccgcggggt ggccagccgg cggaccgtat tgctggcggt tgccatgctg gccgcttcat
      541 tctttccggt tcgtcattcg accgaggtca agccatacgc gatcgatctc ttggtctcgc
      601 tcatgctgat ctcgacgggg tgggccgtcg ggcgtgacct acggtcgcac cgagcctggc
      661 tggcgctctt cggggtgacg atcgtcggtg tttggtgctc gtatgcagca gtgtttccgg
      721 cggccggcgt ggccctgttt ctgggggcga gagttgttcg cgagcgatcg agtcgcctgg
      781 tggcactctg gctgacccac ggtctcttga tgacgctgag ctgggggatc atgttcgtca
      841 ccttcgccca tccgcaggcc cgcgcggccg actttcttcc actcctggaa acctggagag
      901 acgcatttcc gcctttggcg gagccgttgc ggctcccctt ctggctgctc gagattcata
      961 ccggctacat gctggcttac ccgtatggcg gtaacgattt cggcagcgca ctgacgacgc
     1021 tcctggtcgt cgccggaggc atcaggatgg ctcgccgccg cgcgcggcgg cccttgctat
     1081 tcttgctcct cgccccgctg ccagtcgccc tggtcgcggc cgcactgcac cgctacccgt
     1141 atggcaccag cacccgcgtg atgctctaca tggcccccgc attctgcctc ctgaccggcg
     1201 aggggattat gtccctgctc cagcttcgtc agtggtcgag ccgcgggccc ttgatcggcg
     1261 ccggattgct agcgctgatc ccgttagtat gtacggcctt caacgtggcc agcccctata
     1321 aagcgtacga tgacgtgctg cacaggaatc ttgcgcgctg ggtcgccacg cgcaccgcgc
     1381 ccggcgacca atgggtcgtc ttcaatggcg cctctcctcc tccgccagtc aaggacttga
     1441 tggtgatgcc ctggcttcag cgggtgggcg aggcacattt ttacctgttg aagtacgcgc
     1501 ccgtgccgtt gcactgggaa cccgctccgg agacggtcgt gccgaacatc ggcggcaaga
     1561 tctggttgat catccagaat tacggcgacg cggattactt ccccgaaggt cgccttctcg
     1621 cgtaccaagg cgcctttgaa gagcgttttg gccggccgag gaagacgacc cggtttacgt
     1681 tgccgcgcgg tgaaagctgg tctatctgtg agtattcgcc ggcatgtcgc gcggccccgt
     1741 aggcggggtg acgggcgtcc gaagcgattg cgtttggcaa gcccgcgcgg ggtcggcctc
     1801 tctgcgccct ctccgaccga tgatgttgct tgatcgagcg gttcattccg ggtattctta
     1861 acagatgttt aacagttgca cccgcgcggt aacgggaaac cacagataaa catgcgaatc
     1921 atgacatcgc ttgcgatggc agtcctgctt gtcccggtct gccttcggac attcgcctcg
     1981 aatcctgccg acgaccggcc aattagccct gagctcagcc gaaagaccgc catatcaccg
     2041 ctcgaacgcc gcttcggcga caccgtgcgc ccgtttgtgg atagctactg cgtcgggtgc
     2101 cacggcaagg acaagccgaa gggcgatctg gacctgactg tctacacgtc cgccgacgcg
     2161 gtgacgaagg atctggggca atgggaagt
//
LOCUS       DATAQL010001033         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_103638, whole genome shotgun sequence.
ACCESSION   DATAQL010001033 DATAQL010000000
VERSION     DATAQL010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103638"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VHS97_17835"
     CDS             complement(<1..658)
                     /locus_tag="VHS97_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450120.1"
                     /translation="MSSAKTKPLGLRTKLTLWSSLVLAASLAAGFAWVHYGLRRVLDA
                     RNDAFLERTASELLAAVADHTQGGTSDLDSTIRREVMAHEPEGLVVVIRQAGRVSVAP
                     RTAASLRLADQVLPPGIPRTVGLPDAGGRYRVLAARSGSGVLSLEIGISLEETEATLA
                     AFDRLVAGGAVVFLILAVAGGIFLSRQALRPVADSIRAARRLDPANLSERLPRTGADD
                     E"
     gene            complement(639..1310)
                     /locus_tag="VHS97_17840"
     CDS             complement(639..1310)
                     /locus_tag="VHS97_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3450121.1"
                     /translation="MRVLVVEDNPDMGPYLQQGLCEHGFAVDLVTDGNRALDYAATAV
                     YDLVILDRMLPGRDGLDILRRLRAADVRTPAIFLTAKSAVIDRVEGLDGGADDYIIKP
                     FSFAELLARIRVVLRRGPEMQPSVLRVSDLSLDPATHAVERGGQRLELSAKQFALLHY
                     LMRHAGQVVSRTMIQEHVWNYEFDGLTNVVDVHINRLRNKVDRSFDRPLIHTLRGVGY
                     VLRED"
     gene            1496..>2292
                     /locus_tag="VHS97_17845"
     CDS             1496..>2292
                     /locus_tag="VHS97_17845"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3450122.1"
                     /translation="MKQRLAFTLIELLVVISIIGVLIALLLPAVQAARESARRTQCVN
                     NLKQLGVAMHTYESASRSFPPGYVSNFDTSGTDTGPGWGWAAMLLPQFEQKPLFDAIN
                     FNLPIEDVSNSTSRLTRMSVFLCPSDSAPSAWWAVTRDPSGVTLQNICQVAMANYVGM
                     YGTSDPGIDGDGIFFRDSKIGIPNITDGTTQTIAAGERAYALGMATWVGSVTGTSLFP
                     LTDDGVGAPRLEFAPGMILGHAGGNVGPGNPRGEVNQFYSQHPGGVN"
BASE COUNT          458 a          742 c          690 g          402 t
ORIGIN      
        1 gctcgtcgtc ggcgccagtt cggggcagcc gctccgagag gttcgcggga tcgagccgcc
       61 gtgccgcccg aatgctgtcg gccaccggac ggagcgcctg ccgcgacagg aaaataccgc
      121 ccgcgacggc caggatcagg aagacgacgg caccaccagc aacgagccgg tcaaacgcgg
      181 caagcgttgc ttccgtctcc tcgagggaga ttcctatttc gagcgacaga acgccacttc
      241 cggagcgagc ggctagcacg cgatatcgcc cccccgcgtc ggggagccca accgtgcgcg
      301 ggatgccggg tggcaacacc tgatcggcaa gccggaggga ggcggcagtg cggggagcga
      361 cggatacccg gcccgcctgg cggatgacga ccaccagccc ttccggctca tgggccatga
      421 cctcgcggcg gatggtggag tcaagatccg acgtgcctcc ctgcgtatga tcggcgactg
      481 ccgctaaaag ttccgacgcc gtccgttcga gaaacgcatc attcctcgca tcgaggacac
      541 ggcggagccc gtaatgaacc cacgcgaatc ccgccgcgag cgacgcggca aggacaaggc
      601 tcgaccagag tgtgagcttg gtccgcaatc ccagcggctt agtcttcgcg gaggacatag
      661 ccaaccccac gcaacgtatg gatgagcggc cggtcaaaac tccggtccac cttgttcctc
      721 aggcggttga tgtggacatc cacgacgttg gtcaacccgt cgaattcgta gttccatacg
      781 tgttcctgaa tcatagtccg gctcacgacc tgcccagcat gccgcatcag atagtggagc
      841 agggcaaact gcttggccga caattcaagg cgctgcccgc cccgctcgac cgcatgtgtg
      901 gctggatcga gcgagaggtc ggaaacacgc aggaccgaag gctgcatctc gggaccacgc
      961 cgcaggacca cccggattct ggctaataac tcggcgaaag agaaaggttt gatgatgtag
     1021 tcatccgcac cgccgtcgag tccttccacc cggtcgatca ccgcactctt cgccgtcagg
     1081 aagatggccg gtgtccggac gtctgccgcc cggagccgcc ggaggatatc gagcccatcg
     1141 cggcctggaa gcatccgatc gaggatcacc aggtcataga cggcggtcgc ggcgtaatcg
     1201 agggcacggt tgccgtcggt cacaagatcc accgcgaagc cgtgctcgca cagtccctgt
     1261 tgcagataag gacccatgtc cgggttgtct tcgaccacca gcacacgcat gggcgagccg
     1321 cctccggttt caattcgcat agcacagaag cagattataa gccgtcgcga atccgcgatg
     1381 tcggaagatt acagggctgt catcttcgcg caacgccctt gcaatgtctg agcgttaccg
     1441 ttcatcggcg agatcccgat ctcggaagca atctatccgt tccccaaagg gcacaatgaa
     1501 acaaagactt gcgttcacac tgatcgagct gctcgtcgtc attagcatca tcggcgtcct
     1561 gatcgcgctc ttactccccg ccgttcaggc cgcccgcgaa tccgcccggc gtacgcagtg
     1621 cgtgaataac ctcaagcagc ttggcgtggc catgcatacc tatgaaagtg cgtctcgctc
     1681 gtttccgccg ggttatgtct cgaacttcga tacgtccggc accgacacgg gccccgggtg
     1741 gggctgggcc gccatgctcc tgccccagtt cgagcagaaa cccctcttcg acgccatcaa
     1801 ttttaacctg ccgattgaag atgtttccaa ctcgacgagc cggctgacac ggatgagcgt
     1861 gtttctctgt ccctccgaca gcgcaccgtc cgcttggtgg gcggtgaccc gcgatcccag
     1921 cggcgtaacc cttcaaaaca tctgccaggt ggctatggcg aattacgtcg gcatgtacgg
     1981 cacaagcgac cctgggatcg acggcgacgg catctttttc cgtgatagca aaatcggcat
     2041 ccccaatatc accgacggca caacccagac gatcgcggcc ggggaaaggg cctatgcctt
     2101 gggcatggcc acctgggtcg gttcggtcac gggcacgtct ctgtttccgt tgaccgacga
     2161 cggcgtgggc gctccgcggc tcgagttcgc gcccggaatg atcctcgggc acgcgggcgg
     2221 caacgtgggg ccgggcaatc cccgaggcga agtcaatcag ttctatagcc agcaccccgg
     2281 tggagtgaac tt
//
LOCUS       DATAQL010001034         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5142086, whole genome shotgun sequence.
ACCESSION   DATAQL010001034 DATAQL010000000
VERSION     DATAQL010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5142086"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VHS97_17850"
     CDS             <1..1072
                     /locus_tag="VHS97_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450123.1"
                     /translation="SNYLITNDWAPRLRHGYITYDNDNWGFHLLAGQQWSMIIPQEFG
                     IVPRKELIPLTINANYLVGYQFTDNWQIRLVKDFDKKVWLGLSIENPATNLAPGIPAT
                     VNGLAINVTNTGTGGFLTGVPVTPNQAPDIIEKVAWDPNWGHLELLGIQRFFTDNTAC
                     VTAAPTGCAVGTASQKTSFGAGVGGNFFLRVIPHYLELMGGATYGTGIGRYGAGALPD
                     VTIGPDGSLVPLTAFHVWSGIQFYPWESLALYAYAGIEQNNASFFSTFGYGNPVFDNS
                     GCMTPTAATFATGTSATCVADNKRLVDAKIGFWQDLYKGPYGRFVVGAELEYIKRTSF
                     PGIGGVVSTDNIIGFTSLRYYY"
     gene            complement(1123..2928)
                     /locus_tag="VHS97_17855"
     CDS             complement(1123..2928)
                     /locus_tag="VHS97_17855"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3450124.1"
                     /translation="MMLDKAPNGKVSDLLNTLDKALSAGEVEHAVDLFQTDCYWRDLV
                     TFTWNIKTMEGKEQVRDMLKARLSDTKPSNWKIADGEDASETDGITESWIQFETGVAR
                     GFGHMRLKDGRIWTLLTTMSELKGHEEPVGFDRPMGAKHGAERNRKTWKEEREAEAAE
                     LGHSHQPYCVIVGGGQGGIALGARLRQLDVPTIIVEKNERPGDSWRNRYKSLCLHDPV
                     WYDHLPYLPFPRNWPVFSPKDKIGDWLEMYTRVMELNYWGSTECKKASYDEKSREWTI
                     VVKRDGKEIVLRPKQLVLATGMSAKPNMPKFEGKDIFKGDQHHSSQHPGPDKYKNSKT
                     VVIGSNNSAHDICAALWEAGADVTMVQRSTTHIVRSDSLMELGLAPLYSEQAVQSGMT
                     TAKADLIFASLPYKILHEFQIPVYNAIRERDADFYKRLEKAGFLLDYGDDDSGLFMKY
                     LRRGSGYYIDVGASELIANGSIKLKSGVDVKKLTEHSVILSDGTELPADLVVYATGYG
                     SMNGWAADLISREVADKVGKVWGLGSNTTKDPGPWEGEQRNMWKPTQQEGLWFHGGNL
                     HQSRHYSQFLSLQLKARMEGIPTPVYGLQKVHHLS"
BASE COUNT          655 a         1030 c          965 g          780 t
ORIGIN      
        1 atcaaactac ttgattacca acgactgggc cccgcgtctg cggcacggct atattaccta
       61 cgacaatgac aactggggtt tccacctgct cgccgggcag caatggagca tgattatacc
      121 ccaggagttc gggatcgtgc cccgaaagga gcttattccg cttacgatca acgcgaacta
      181 tctcgtcggc tatcaattta ccgacaactg gcagatccgg ctcgtgaagg atttcgacaa
      241 aaaggtttgg ttgggccttt caatcgaaaa ccccgcgaca aacctcgctc ccggcattcc
      301 cgccaccgtc aacggcttgg ccatcaacgt caccaatacc ggcaccggag gtttcctcac
      361 cggcgtgccc gtcaccccga accaggcgcc cgacattatc gaaaaggtcg catgggatcc
      421 caactggggt catctcgagt tgctcgggat ccaacggttc tttaccgaca acaccgcgtg
      481 cgtgaccgcg gcgccgaccg gctgcgcggt cggaacggcc agccagaaga cctccttcgg
      541 cgccggtgtg ggcggaaact ttttcctgcg ggttattccg cactatctcg aattgatggg
      601 gggcgcgacg tatggcaccg gaattggtcg ctatggggca ggggctttgc ctgacgtgac
      661 cattggcccc gacggctcgc tcgtcccgct caccgcgttc catgtctggt cggggataca
      721 attttatccc tgggagagct tggcactcta tgcctatgcc ggcatcgagc agaataatgc
      781 gagcttcttc agcacgttcg gctatggcaa tcccgttttt gacaacagcg gctgcatgac
      841 tccgacggct gcgaccttcg ccaccggcac gtcggcgaca tgcgtcgccg acaacaagcg
      901 cctcgtggat gccaagatcg gcttctggca ggatctctac aagggacctt acggtcgatt
      961 cgtggtcggc gccgagttgg aatacatcaa acgtacgtct ttccccggca ttggtggcgt
     1021 cgtatcgacc gacaatataa tcggctttac ctcgctgagg tactactact agtctggtta
     1081 tacggcttgc ggagcgcggc cgaggctgcc gcgtcacatc gatcagctga gatggtggac
     1141 cttctgaaga ccgtaaaccg gtgtgggaat gccttccatt cgcgccttca actggaggga
     1201 cagaaactgc gagtagtggc gtgactggtg caggttaccg ccatggaacc aaagcccctc
     1261 ctgctgcgtc ggcttccaca tgtttcgctg ctcgccttcc catgggcccg ggtctttggt
     1321 cgtattcgag cctagtcccc agaccttacc taccttgtcg gctacttccc tggagatcag
     1381 gtcagcggcc cagccgttca tcgaaccgta gccggtggcg taaacgacga gatcggccgg
     1441 cagctcggtc ccgtcgctca agatgacgga atgctcggtg agttttttga cgtcgacgcc
     1501 gctcttcagt ttgatgctgc cattggcgat cagctccgaa gcaccaacgt cgatgtagta
     1561 tccggacccg cgccgcaggt atttcatgaa cagaccagag tcgtcgtcac cataatcgag
     1621 caggaagcct gccttctcga ggcgtttgta gaaatcggcg tcgcgttccc tgatcgcgtt
     1681 ataaaccggg atctggaact cgtgcagaat cttgtagggc agtgaagcaa agatcaggtc
     1741 ggctttggcc gtggtcattc cagactggac cgcctgttcg gagtaaagcg gcgccaaccc
     1801 gagctccatc aacgagtccg acctcacaat gtgggtggtc gagcgttgaa ccatagtgac
     1861 gtctgctcca gcttcccaca gcgcggcaca aatatcgtgt gcggaattgt tcgagccgat
     1921 cacaacggtt ttgctgtttt tgtatttgtc cggccccgga tgctgcgacg aatggtgctg
     1981 atcgcccttg aaaatatcct tgccttcgaa tttcggcatg ttgggcttgg cggacattcc
     2041 ggtcgcaagc acgagttgtt ttggcctgag gacgatttcc ttgccgtcgc gctttacgac
     2101 aatggtccat tcgcggcttt tctcgtcgta agatgccttc ttgcattcgg ttgagcccca
     2161 gtaattgagc tccatcaccc ttgtatacat ttcgagccag tccccgatct tgtccttcgg
     2221 tgagaacacc ggccagttcc ggggaaacgg cagatagggc agatggtcat accagaccgg
     2281 atcatgcagg cagagcgact tgtaacggtt gcgccagctg tcgccaggcc tttcgttctt
     2341 ttcgacgatg atcgtcggca catcgagctg gcgcaaccgg gctccgagcg cgatgccgcc
     2401 ctgaccgcca ccgacgatga cgcaataggg ctgatgcgag tggcccagtt cagccgcttc
     2461 cgcctcgcgt tcttccttcc acgtttttcg attgcgttcg gcgccgtgtt tcgctcccat
     2521 cggacggtcg aaaccaactg gctcttcgtg ccccttgagt tcgctcatgg tcgtcagcaa
     2581 cgtccatatc cggccgtcct tcagacgcat gtggccaaag ccgcgggcga ccccggtttc
     2641 gaattgtatc cagctctctg taattccatc cgtttcagaa gcgtcctcac cgtccgcaat
     2701 tttccagttc gagggcttgg tatcggagag ccgcgccttg agcatgtcgc gaacctgttc
     2761 cttgccctcc atcgtcttga tgttccaggt gaaggtcacg aggtcgcgcc agtagcagtc
     2821 cgtctggaag agatcgacgg cgtgttccac ctctccggcg gagagcgcct tgtcgagcgt
     2881 gttgagcaga tcagacactt ttccgttcgg agccttgtcg agcatcatgt cctcccttgt
     2941 gccgatggca gagccctgcg ctcttttttc tttgttccgg cgaactgaag ccgaggcgcc
     3001 aacgaaagca atcagctcgc gctgtgcatc gatccttcag gacagactaa atcctagcac
     3061 tttgcggcca agaggcgagc gagaaacagc ccgatcgggc cgcgcgaata aagctctcaa
     3121 tccggagaac ggcagcagca cctccatcac ctgctggccg tagcgtggct gctgcacgtc
     3181 gctgatctgg ctgcggccgc catagctgag gcgggcctgc gcgatcttgc tgcatcggta
     3241 ttgtcgcgct ggatgtcctt cgggggatgc cggcggcgat attcgtcgcg atccggaacg
     3301 atccgccgtc tgaccgatta gctccgattg atccggagat taaatcatga gcgatcaatt
     3361 ccagccaccg ccctacgtct acgacgggtt ggatccagtc cgggagagca agtcgatctt
     3421 cgcggaggcc
//
LOCUS       DATAQL010001035         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3461081, whole genome shotgun sequence.
ACCESSION   DATAQL010001035 DATAQL010000000
VERSION     DATAQL010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461081"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VHS97_17860"
     CDS             complement(<1..392)
                     /locus_tag="VHS97_17860"
                     /inference="COORDINATES: protein motif:HMM:NF022698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HEX3450125.1"
                     /translation="MAGYSGTPLAKKLGIQAGAFVRAVDAPDDYAQLLEPLPANVTFV
                     ERGADELDMVHLFTRSRSKLVKYLDEYKKKIKQNGSIWVSWPKRSSGIPSEVTEDTIR
                     EVALPLGLVDVKVCAVDDTWSGLKLVIR"
     gene            579..1397
                     /locus_tag="VHS97_17865"
     CDS             579..1397
                     /locus_tag="VHS97_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX3450126.1"
                     /translation="MFGFFKERRRKRLRSATFPHTFLEIIERNVSFYKCLPDADRREL
                     QGLVQVFLGEKVFEGCGGLELTDEIKVTIAAQACLLLLHRQTDIYPRLITILVYRSAY
                     LAKAALPIGGGAVLEGDAIHLGESWSSGVVVLSWDDVRAGASDIHDGQNLVLHEFAHQ
                     LDREDGAINGTPLLDQRSQYVAWARVLNVEFERLRRDRWLGRATVLDEYGATNPAEFF
                     AVATECFFEKPRVLQKRHPELYDELKRFYRQDPAQLLPARPGPATGELNEASVP"
     gene            complement(1375..2184)
                     /locus_tag="VHS97_17870"
     CDS             complement(1375..2184)
                     /locus_tag="VHS97_17870"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX3450127.1"
                     /translation="MSELPTNPFNPPSSEIGQLGDVGVSKLAQTGAGPRPWGPWATLG
                     LTLLCAVLLFAIQVAVLLVFMLIRVARGAEPKFDDVASNGNVVAVGTMASTAAIVGLV
                     ALLVHLRRYPIRDYLALVWPPARSALIAFAGLVVVLFSSDLLSYMLGRPLVPEVMVDL
                     YRMAWLPGLLPALVVFAPIGEETLFRGFLFKGIAASRAGPIAAIIISAIAWAPLHVQY
                     DWYGVGTIAVMGLFLGVVRHATGSLYLTMLLHAVANAVATLEIVIKEHWLR"
     gene            2257..>2529
                     /locus_tag="VHS97_17875"
     CDS             2257..>2529
                     /locus_tag="VHS97_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450128.1"
                     /translation="MSIRSYQTGDEHAQARIYNTVAGPLPSFKPSTPEEIARRYQPAD
                     ADSTTRYYAIENGAVVGYVAFGSNGRVSYPWCLPGAESYQEPLLKTM"
BASE COUNT          559 a          803 c          705 g          462 t
ORIGIN      
        1 ttacgaatga ccagtttgag ccccgaccac gtgtcgtcga cggcgcacac cttcacatca
       61 accaaaccca gcggcagtgc gacctcgcgg atcgtgtctt cagtgacttc cgacggaatt
      121 cccgacgacc tcttcggcca cgaaacccag atcgagccat tctgcttgat cttttttttg
      181 tactcatcca aatatttaac gagcttcgat cgcgatcttg tgaagagatg aaccatatcc
      241 agctcatccg cccctcgttc aacaaacgtc acgtttgccg gcaaaggctc aagaagctgg
      301 gcgtaatcgt ccggagcatc caccgcccgt acgaaggcgc cggcctgaat ccccagtttt
      361 ttcgccaggg gcgttcccga gtatcccgcc acgagtcagt acccctcaat gctcaccggc
      421 aaggagctgt ttacccgcct gaccaatccg catgttattc tatctcttga gtcgaaagca
      481 tgctcgacca atgacggcga tcgtccgtcg cgaacgagac gcgagggaat agatcgcgaa
      541 acgaagcgtc cagcggaact ccacacaagg atcaggtcgt gttcggcttc ttcaaagagc
      601 gtcggcggaa gcggttgcga tcggcaacgt ttccacatac cttcctggag atcatcgaaa
      661 ggaacgtttc tttttacaag tgcctcccgg atgccgatcg ccgagagctc caaggattgg
      721 tgcaggtttt tctgggagaa aaggtgttcg agggctgtgg cggactcgag ctcaccgacg
      781 agatcaaagt gaccatcgcc gcccaggcct gcctgctctt gctccatcgt cagaccgaca
      841 tttatccccg gctgatcacc atcctggtct accgcagcgc ctacctcgcc aaggccgccc
      901 taccgatcgg cggcggtgcg gtcctcgagg gagatgccat ccacctgggc gaatcctgga
      961 gtagcggcgt ggtcgtccta tcctgggatg acgtgcgcgc cggagcgtca gacatccacg
     1021 acggacaaaa cctcgtgctg cacgagtttg cccaccagtt agaccgagaa gatggcgcga
     1081 tcaacggcac accgctcctg gatcagcgca gccagtacgt cgcatgggct cgtgtgctca
     1141 acgtcgagtt tgagcgcctg cggcgcgatc gctggctggg ccgcgcgacc gtgctcgacg
     1201 aatacggtgc aaccaatccc gccgagtttt tcgccgtcgc cacggagtgc ttctttgaga
     1261 agccgagagt gcttcagaaa cggcatcctg aattatatga cgagctgaag cggttttacc
     1321 gtcaagaccc tgcgcagctc ttgccggcta ggcccgggcc ggcaaccgga gagcttaacg
     1381 aagccagtgt tccttgatga cgatttcgag cgtcgccacg gcgttggcaa cagcatgcag
     1441 cagcatcgtc aagtacagtg accccgtcgc atgccgaact acgcccagga acagccccat
     1501 gaccgcgatt gtgccgaccc cataccagtc gtactgaacg tgcagtggtg cccaggcaat
     1561 tgcactgatg atgatcgccg cgatcgggcc cgcgcgcgag gcggcaatcc ccttgaagag
     1621 aaacccgcga aaaagcgtct cctcaccgat aggtgcgaac accaccaggg caggcagcaa
     1681 ccccggcagc catgccattc ggtaaaggtc caccatgact tcaggaacga gcgggcggcc
     1741 cagcatgtac gagagcaaat cgctgctgaa caacacgacc accagacccg cgaaggcgat
     1801 cagtgccgat cgcgccggcg gccagacaag cgccaggtag tcgcggatcg ggtagcgtcg
     1861 taagtggaca agtagcgcga ccaatcccac gattgccgcc gtacttgcca tggttccaac
     1921 ggcgacgaca ttgccgttgc tcgcgacgtc atcgaatttg ggctcagcac cccgcgcgac
     1981 ccggatcagc atgaagacga gcaatacggc aacctgaatg gcaaacagca gcacggcgca
     2041 gagcaacgtc aagccgagcg tcgcccacgg cccccacggc cgaggacctg cgccggtttg
     2101 cgcgagcttc gacaccccca cgtcgcccaa ctggccgatt tcagaggagg gagggttgaa
     2161 tggattcgtc gggagttcgc tcatgggcgt tgacatcacc gagccggcga tctcaagatt
     2221 gggcgcaacc gccatttttc acacaggacc tccccaatgt cgattcgatc gtatcagact
     2281 ggcgatgaac acgcacaggc ccggatctac aatacggtcg ccgggcctct gccgtcattc
     2341 aaacctagca ctccggaaga aatagcccgg cgctaccagc ccgccgatgc agactcgaca
     2401 acaaggtatt acgccatcga aaacggcgcc gtcgtcggct acgtcgcttt cggctccaat
     2461 gggcgcgtca gctacccgtg gtgtctgccc ggggccgagt cataccaaga gcctctcttg
     2521 aaaacaatg
//
LOCUS       DATAQL010001036         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2545294, whole genome shotgun sequence.
ACCESSION   DATAQL010001036 DATAQL010000000
VERSION     DATAQL010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545294"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VHS97_17880"
     CDS             complement(<1..281)
                     /locus_tag="VHS97_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3450129.1"
                     /translation="MAVTPRIQLNDADGVKVVRFQDNQLFDERTVREAADQIAAALPN
                     DGSPIRLVVDFSDVSLISSTFLSKLILLQRRVDATRGKLRLCEMTPIVQ"
     gene            complement(353..838)
                     /locus_tag="VHS97_17885"
     CDS             complement(353..838)
                     /locus_tag="VHS97_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3450130.1"
                     /translation="MLKPSIETHNVNGILVAEFWESLRLDPAPVQDLRGHYEAHLKNH
                     GTADVVIDLSGVGFAGSAALGNFVALQRLVRAKGGRIVFCNVDPTVTEVFRASKLDSL
                     FEFAADRADAVRAISERLPTASSDRKAAAGDPAQPVKSPGAGRSGSGPLRRRNHGQES
                     T"
     gene            1274..2278
                     /locus_tag="VHS97_17890"
     CDS             1274..2278
                     /locus_tag="VHS97_17890"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX3450131.1"
                     /translation="MRLFLLLPSLFLGFTAAGQTLGGDWIPAVFPERAFDFGNVARGS
                     QVRHSFPVVNRTNSDVHIVDWRTKCGCTNVKVGARLIPPGTQTTIEATIDTTRFQGPK
                     ASGLTLVIDRPNSIEVDLNLTCFIRADVDLAPGQIDFGTVRRSDKLPSAALTLTYRGG
                     KPGWEIAQMKTQSVKVKAIAERLDRAGGGLNQWTVTATLQPDLNNGYFKDEITLFTND
                     SPPQSIPISVVANIQSAVRVTPSIINFGPVRACQSVTKTVHVRSSSSFIITKLDADRA
                     DLTAVEQQTGSLPEHTVNITLQGPAEPGPFHGVVKIDSDLKDEPTAQIKAFATIMPAP
                     "
     gene            2484..>3303
                     /locus_tag="VHS97_17895"
     CDS             2484..>3303
                     /locus_tag="VHS97_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450132.1"
                     /translation="MRLSRLISRRWVTLLWLVLLAVAFMMWSVQPWILSALLGFDEFQ
                     DDGRMAVLKLWGAGRLAPMTTVGLLSLFPVLDVSLAVVLLAAIFVLVIKLFERAPDSQ
                     RVSWLSANLPQSPISRSRFRFCTVIGLIALLGLEYGWEVAEWKKWQQRERYLGLADGF
                     ASSAVQSRKRFRRIHAELTRLDEDGAAAPDDASATDALSRQRYRFETHLVYTFALAKA
                     FDERAKKYAEAAKQPLQPIRPDPPLPQKPSDSTPTAEGYSRDHGRILAVYNELSR"
BASE COUNT          681 a         1007 c          932 g          683 t
ORIGIN      
        1 tgttgaacga tcggtgtcat ctcgcagagt ctcagcttgc cgcgggtcgc gtcgacccgg
       61 cgttgcagca ggataagctt gctgaggaat gtactcgaga tcaggctgac gtcggaaaaa
      121 tcgacaacga gtcgaatggg gcttccatca ttaggcaaag ctgcggcgat ctgatcggcg
      181 gcctcgcgga cggtgcgttc gtcgaacaat tggttgtcct gaaaacggac gactttgacg
      241 ccgtcagcgt cgttgagctg aatcctgggt gtgacagcca ttgctatgga ccccgcaaaa
      301 gaaggattcg atttcgggtc gcgcggaccg cttgatttct cgccgaccct acttaagttg
      361 attcctggcc atggtttcta cgtcgtaatg ggcccgagcc gcttcgtccg gctcccggcg
      421 actttacagg ttgagctggg tcgccagcgg ccgcttttcg atccgacgaa gccgtgggga
      481 gtcgctcgct gatggctcgt acggcgtcgg ctcgatcggc agcgaattcg aagagtgaat
      541 cgagcttgct cgctcgaaaa acctcggtaa ctgtgggatc gacgttgcaa aagacgatcc
      601 gccccccctt ggcacgcacc agtcgttgca aggccacgaa atttccgagc gccgccgagc
      661 cggcaaatcc cacaccgctg agatcaatga ccacatcggc agtcccgtga ttcttcaggt
      721 gggcttcgta atgcccgcgc aagtcttgca ccggcgccgg gtcgagccgc aggctttccc
      781 agaattccgc gaccaggata ccattcacat tgtgagtttc tattgagggc ttcaacatca
      841 atcacctgcg cggcgaggtt ttttggttga attcaacgaa tccgggacga accccaagaa
      901 agaaaggaaa ggattcgacg ggcgacctaa ccggtcgacc atcgagcgcg gccggagttt
      961 tccttctcgg tctgaaaaat ccaggctggg cacgacctcc ggaacgactg gccgcgcttt
     1021 gcggggagcc agcacagacg gaatcatcgt agcagagtcg ggtgatggca agccagtcgg
     1081 agaaccggaa tcgggctcga cggcaaatcg aaccgccgcc acaatccatt caaccgactt
     1141 gacaacgccc cggaaaatgg tgcactcgat caccgcaaaa atgacgacgg ccgattcttc
     1201 ttgaatctcc gtcacgaggc gcgcgacttt cgcggagtca gtcgactttc gaacaaggca
     1261 gggagtggcc ccgatgcgtt tgttcttgct acttccgagt ttgtttctgg gtttcacggc
     1321 cgccgggcaa acccttggcg gtgactggat cccggcagtc tttcctgaac gcgctttcga
     1381 cttcggcaat gtcgcccgcg gatcccaggt tcgccactct tttcccgtgg tcaatcgcac
     1441 gaattctgac gtccacatcg tcgactggcg aaccaaatgt ggctgcacga atgtcaaggt
     1501 cggggccaga ttaattccgc ctggcaccca aaccaccatc gaagccacga tcgataccac
     1561 gaggtttcag ggccccaaag catcagggct gaccctcgtt atcgaccggc ccaattcaat
     1621 cgaggtcgac cttaacctca cctgctttat tcgagccgac gtcgacctag cccccggaca
     1681 gatcgatttc ggcacagtcc ggcgctccga caagcttccc tcggccgcat tgacattgac
     1741 ctatcgcggc ggcaagccgg gctgggagat tgcccaaatg aagactcaaa gcgtcaaagt
     1801 caaggccatc gccgagaggc ttgatcgtgc cggcgggggc ctcaaccagt ggaccgtgac
     1861 agcgaccctc cagcccgatc tcaacaacgg ctacttcaag gacgagatca cgctcttcac
     1921 caatgactcg cccccccagt cgatcccaat atcggtcgtt gccaacatcc agagtgctgt
     1981 ccgcgtgacg ccgtcgatta tcaatttcgg accggttcga gcgtgccaat ccgtcaccaa
     2041 gacggtgcac gtacgatcct catcgtcctt tatcatcacg aaactcgatg cggatcgcgc
     2101 cgacctcacg gccgtcgagc aacagactgg ttccttgccg gagcacacgg tcaacatcac
     2161 gctccaaggg ccggccgagc ccggtccttt tcacggcgtc gtcaagatcg acagcgattt
     2221 gaaggatgaa cccacggctc agatcaaggc gttcgccacg atcatgcccg ccccataggt
     2281 cgcattggcg cgcgacgccc tcaccacttc cacacgcttg taagggcgcc cctacttgtc
     2341 aacgtagcct cgtggcctgt ctggcaaccc cgatgggatc tggcgttcct ggacgtggtg
     2401 gcatacgatg tgcgcccaga ccgacgaacc caggagcgat acgcgacaaa catcaacgag
     2461 tcctcgtcac ggagacgtta accatgaggt tgtctcggct gatatcgagg cggtgggtta
     2521 ccctactgtg gctcgtattg ctcgcagtgg cgtttatgat gtggtcggtc cagccctgga
     2581 ttttgtcggc attgctgggg ttcgacgaat ttcaggacga tggccgcatg gcagttctca
     2641 agctgtgggg cgcgggccgt ctggccccca tgacaaccgt aggtctgctt tccctgttcc
     2701 cggtccttga tgtatcgctg gccgtcgtat tgctcgcagc aatattcgtc ctcgtgatca
     2761 aactttttga acgagcaccc gatagccagc gggtctcttg gttgagcgca aatttaccgc
     2821 agtcgcccat aagccgctct cggttccgct tctgcacggt gattggcttg attgcgttac
     2881 tcgggctcga atacggatgg gaagtggccg aatggaagaa atggcagcag cgcgaacgat
     2941 atctgggcct ggcggatggc tttgccagca gtgcggtcca gtccagaaag cggtttcggc
     3001 gcatccatgc cgagctgact cgtcttgacg aggatggtgc cgccgcgccc gatgacgcct
     3061 cggcgaccga cgccctgagc cgccagcggt atcgcttcga gacacatctc gtctatacgt
     3121 tcgcgctcgc aaaggcattc gacgaacgcg cgaaaaaata tgcagaggct gccaagcaac
     3181 cattacagcc aattcgcccc gacccgccgc tgcctcagaa gccgtctgac tcaacgccca
     3241 ctgccgaggg ttattcccgc gaccacggcc ggattttggc cgtctataac gaattgagcc
     3301 gtt
//
LOCUS       DATAQL010001037         5895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2089304, whole genome shotgun sequence.
ACCESSION   DATAQL010001037 DATAQL010000000
VERSION     DATAQL010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089304"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2446
                     /locus_tag="VHS97_17900"
     CDS             <1..2446
                     /locus_tag="VHS97_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450133.1"
                     /translation="RAQSEAERVRGRAWSSFPEAGQLADRLVQIAMELPPEPPETVYN
                     SFKQSVRGGEGLSLGGTASGASSAGESAAAPPAGRTPVLQAGDELSGLGTVIQGLRAI
                     LTRAGQSIAGLKEKAQWDWIDHSALLRKLVREFREGDPERALRRAIPITAPGDRTQSA
                     TANQLPWSRAIYSLGELLKRQGRGEATPVLPAEPGLVQLLTREYHRAAKRAIDQGDFR
                     RAAYIYGVLLREDRLAAGALQRGGLHHDAAIIYQRKLNDKAAAAQAFEAAGAIDRAVA
                     LYRQMGRHEAAGDLLRRVGEDDLALVEYAAAAALLAGLTPPDHLAAGRLWLEKARCPD
                     QAIGAFQTGWSRRPEGNAALCGLELVRLHSQRGAIEPIRALLDQVDAHFETFGKSFDG
                     FFYNEVVRLAGVPSMAPFTDELRDRALQSLARRLRQGVEGYQSAPSLVAALLGRTKLW
                     PTAVVSDAEFAATSEQKRSRAVAATPIHDPRITRVQIERGTVTAVCQSKVTGELYLGF
                     AGGQVIAYRADRNQAVKVGEGAGVVAGLAVDPTGQTLVTLRQFDREAVLSSFRKHPDG
                     SYRHRPDVHFSVSPQSWLTPVLRLGVERLVGLSDGRDLHIIDAASGILWQRRRISVSA
                     PLAPWSALLLPADSPRAWPHSRLMILTHDRERWIIVDEDGELLHMTFYDWQPGIPESS
                     SLRSAPVSWQIVPPFIELLGLDITGAVYGARFHGEGRKIEVVSMQVARTPEGYLAAAR
                     AGADTVVAVSRSRIDWLDRSADHFHVKAKMNVDFPTAVACFPSPGAQETLVVCSDGMI
                     AHVAPPRRARVGQMDG"
     gene            2645..2914
                     /gene="rpsO"
                     /locus_tag="VHS97_17905"
     CDS             2645..2914
                     /gene="rpsO"
                     /locus_tag="VHS97_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX3450134.1"
                     /translation="MAITKEKKQEIIGTFGRAEVDTGSPEVQVALLTARINDLTGHFK
                     THAKDHASRRGLLMMVSKRSSLLKYLRLHDRKKYLEVISRLGIRK"
     gene            3319..5697
                     /locus_tag="VHS97_17910"
     CDS             3319..5697
                     /locus_tag="VHS97_17910"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX3450135.1"
                     /translation="MSSNLISRKLVERTVGGKLLSIETGHLAKQASGSVVVRIGDTMT
                     LVALVAAPGREGLDFFPLSVDYREKTYAAGKFPGGFIKREGRPTTKEILTSRLIDRPI
                     RPLFPDWYRDEVQIQAGPISADRQNDADVPSMIGASAALLLARVPFLGPVGSIRLGRV
                     DGKLVAFPTAEELEKSDLDLIVSSTEKAVVMIEGFGEELTDGEMGDAIMEAHRLNQEL
                     IELQKELIAAVGLPPHEHPAVTIDPLRQTIYDRYGEQFREVKQIVMKAERNAAGKALL
                     NTILAELIPADPKAAAPWPATPDVEKSPHDDPAPSPLPADAEVTPARIKAAYSAVEER
                     VVRELILDGKRPDGRGPKDLRSIKCEVGLLPRAHGSALFQRGETQALVTTVLGTGADE
                     QRVDGIMDEYSKKFMLDYNMPSFAVGEVRPIRGPGRREIGHGALAERSVASILPSPAR
                     FPYTIRCVSDILESNGSSSMASVCGATLSLMDAGVPISDPVGGISIGLVQDESTGRHI
                     LLTDIIGDEDHFGDMDFKVAGTQHGVTGIQLDLKNRGITEEIIRETLEQAHEARVEIL
                     RAMLRSIKRPREEISANAPRLIQIQINPEKIGMVIGPGGKTIRRLQEETGAKIDIDDT
                     GVVTLSSLEAAGAEAARDKIVAMTEGVQVGRIYEGRVTSIKEFGAFVEILPGKDGLVH
                     ISELSDGYVSNVTEVCRVGDPMLVKAIAVDDQDRVKLSRKAALAERGQVDELAGRTRP
                     APTGGGGDRGGPGDRGGPGSGPPPRRPPNGGDRDRDRGYGGDRGGPRGPGRP"
BASE COUNT         1060 a         1781 c         1963 g         1091 t
ORIGIN      
        1 gcgcgctcaa tctgaggcag agcgagtgcg cggccgcgcg tggagctcgt ttcccgaggc
       61 gggccagctt gccgaccgac tcgtacagat tgccatggag ctgccgcctg agccgccgga
      121 aacggtttat aactcgttca aacagtcagt gcgcggcggc gagggattgt cccttggggg
      181 cacggcctct ggggcgtcgt ccgcgggcga gtcggccgcg gccccgccgg ccggtcgaac
      241 gccggtactg caggctgggg acgagctctc cggcttggga accgtgatcc aagggctgcg
      301 ggccatcttg acccgggcgg ggcaatcgat cgcggggcta aaggaaaagg cacagtggga
      361 ttggatcgac cattccgccc tgctgcgcaa actggtgcgc gagttccgcg aaggggaccc
      421 cgagcgagcc ctacgtcggg ccatcccgat cacggcgccc ggagatcgca cccaatccgc
      481 cacggccaat caattgccgt ggagcagggc aatctacagc ctgggcgagc tcttgaagcg
      541 acagggacgt ggtgaagcga cgcccgtatt gccggccgaa ccgggcctgg tgcaattgtt
      601 gactcgagaa tatcacagag ccgcgaagcg ggcgatcgac caaggtgact tccgtcgtgc
      661 ggcatacatc tacggcgtgc tgctacgtga ggatcgcctt gccgccggcg ccttgcagcg
      721 gggagggctg catcatgacg cggcgattat ctatcagagg aaattgaatg acaaagccgc
      781 cgccgcccag gcgttcgagg cggccggcgc gatcgatcga gcggtcgccc tctatcgcca
      841 gatgggccga catgaggcgg ccggcgacct gctgcgccgc gttggtgagg acgatcttgc
      901 cctggtcgaa tacgcggcag ccgcggctct ccttgccggc cttacacctc ctgatcatct
      961 cgcggccggc cggttgtggc tagaaaaagc ccgttgtccc gaccaggcga tcggggcatt
     1021 ccagacgggc tggtcgaggc gtcccgaagg caacgcggcc ctgtgcggat tggagctcgt
     1081 gcggcttcat tcccagcgcg gcgcgatcga gccgatccgg gcactgctcg atcaagtcga
     1141 tgcgcacttt gaaacgttcg gcaaatcgtt cgatggattc ttttacaatg aggtggttcg
     1201 gctggctggg gtcccctcga tggcgccatt caccgacgag ctgcgcgacc gagcgctgca
     1261 atcgcttgcc agacgactgc gccagggtgt cgaagggtac cagtccgccc cgtccctggt
     1321 ggcggctctc ttgggccgga cgaagctctg gcccacggcc gtggtgagcg atgccgagtt
     1381 tgccgccaca tccgagcaga agcgctctcg cgccgtcgca gccacgccaa tccacgaccc
     1441 gcggatcacg cgggttcaga tcgagcgtgg gactgtcacc gcagtttgcc agtcgaaggt
     1501 gactggtgag ctctatctgg gcttcgccgg cggccaggtg atcgcttacc gggcggaccg
     1561 caatcaggcg gtcaaggtcg gcgagggggc tggtgtggtc gccggtctgg ccgtggaccc
     1621 gacgggccag accttagtca cgctgcggca gttcgaccgt gaggcggttt tgtcttcttt
     1681 tcggaaacat cctgatggga gctatcgcca tcgtccagac gtgcattttt cggtgtctcc
     1741 gcaaagctgg ctcaccccag ttttgcggct gggtgtcgag cggctggtcg gactgtccga
     1801 cggccgggac ttgcacatca tcgacgccgc atccggcatc ctctggcagc gccgccggat
     1861 ctcggtgagt gcgccgttgg ctccatggtc cgccctcctg cttcccgcag attcgccgcg
     1921 cgcttggccg cacagtcggc tgatgatctt gacccacgac agagagcgct ggatcatcgt
     1981 cgacgaggat ggcgaattgc tccacatgac gttttatgat tggcagcctg ggatccctga
     2041 atcgagctcg ttgcgatcgg cgccagtctc gtggcagatt gtccctccgt tcatcgaatt
     2101 gctcggcctc gacataaccg gggctgttta tggggcgagg tttcatggcg aaggaagaaa
     2161 aatcgaggtc gtgtcgatgc aggtcgccag gacgcccgag ggctatctgg cggctgcgcg
     2221 cgcaggcgct gatacggtcg ttgctgtgtc ccgtagccgg atcgattggc tcgaccggtc
     2281 cgccgaccat tttcacgtga aggcaaagat gaacgtcgat tttccgaccg ccgtcgcctg
     2341 tttcccgtct ccgggagcgc aagaaacgct ggtagtctgt tcggacggca tgattgctca
     2401 cgtggctccg ccgcgtcgag cgagggtggg gcaaatggac ggatgaacga gagagtggga
     2461 gtgattcgat gtcaggcaag ctgatctccg gaggcgattc gactttccgc cggaacacct
     2521 gttgggctct tgtgttgcga aggggattgc aaacggtcgt gtctttccct atgcttgaga
     2581 gcttaaagtg taggtcaaac gttcagaaac ggctttgaga aggccggtgg tattggggcg
     2641 cgagatggcg atcacgaagg agaagaagca ggagatcatc gggacatttg gccgggccga
     2701 agtcgatacg gggtcgcctg aagtgcaagt tgctttgctg acggcccgga tcaacgactt
     2761 gaccggtcac ttcaagacgc acgccaagga ccatgccagt cgccgcgggc tactgatgat
     2821 ggtgtcgaaa cggtcgagtc tgctgaagta tctgcggctc cacgaccgta agaagtacct
     2881 tgaagtgatc agccggctgg gaattcgtaa gtgaccggct ggccttttag ttgcggcgtg
     2941 ggctcgcggt cttttcatgt tcatgccacg cggtgagtag taccagggcg tgccggcgcg
     3001 agattgcgga ggccggccca tagcgaccga ttcccggcct acgcctcgat ggtccctcct
     3061 gttatttttg gctctttcgt gacgtcttcg tttccgtcag gtatccactc tcgctgactt
     3121 ctagtgcgcg atcgagcaac gatcgattcg ggcaaacgga tttttcgtgg agccgaatcg
     3181 agttccgtgc cgcgatcgga ccgagagtcg aggcagagac gccgacaaac gaaggagtcg
     3241 gtagagagcg ccgcccaccg ggccattttc attgccatca ccacgccgcg catgggcgcg
     3301 gtttcgcagg tagagtccat gtccagcaat ttgatttcgc gcaagcttgt cgagcggacc
     3361 gtcggcggca agctcttgag catcgagacg ggtcatctcg ccaagcaagc cagcggttcc
     3421 gtggtcgtgc gaatcggcga cacgatgacg ctggttgccc tggttgccgc gcccggtcgg
     3481 gaagggctcg atttcttccc cttgtcggtc gattaccgcg agaagacgta tgccgccggt
     3541 aagttccccg gcggctttat caagcgtgag ggccggccca ccaccaagga aatcctcacc
     3601 tcccgactga tcgaccggcc gatccgaccg ctctttcccg attggtatcg cgatgaggta
     3661 cagatccagg cgggcccgat ttcggcggat cggcagaatg atgccgacgt gccgtcgatg
     3721 atcggcgcct ccgcggcgct tctgttggcg cgggtccctt tcctgggacc ggtgggttcg
     3781 atccggctcg gccgcgtgga cggcaagctc gttgcattcc ccaccgcaga agaactcgag
     3841 aagagcgatc tggacctgat cgtctcgagc accgaaaaag cggtcgtcat gatcgaaggg
     3901 ttcggcgaag aattgaccga tggcgagatg ggcgacgcca tcatggaagc gcaccggttg
     3961 aaccaagaat tgatcgagct gcaaaaagag ttgatcgccg cggtcggctt gcccccgcac
     4021 gagcaccccg ccgtgaccat cgacccgttg cggcagacga tctacgaccg ctatggcgag
     4081 cagttccgcg aagtcaagca aatcgtgatg aaggccgagc gcaacgcggc cggcaaggcg
     4141 ctcttgaaca cgatcctcgc cgaactcatc ccggccgacc ccaaggccgc ggcgccttgg
     4201 ccggccacgc cggacgtcga aaagtccccg cacgacgacc ccgcccccag cccgctgccg
     4261 gccgacgcgg aagtgacgcc ggctcggatc aaggctgcgt atagcgcggt cgaagagcgg
     4321 gtcgtccgcg agctgatctt ggatggcaag cggccggatg ggcgcggccc gaaagactta
     4381 cggtcgatca agtgcgaggt cggtctgttg ccccgagcac acggctcggc gctatttcag
     4441 cgtggagaga cccaggccct ggtgaccacg gtgctgggga ccggcgccga cgagcagcgg
     4501 gtcgacggta tcatggacga atacagtaaa aagttcatgc tcgactacaa catgccgtcc
     4561 ttcgccgtcg gcgaggtccg gccgattcgt ggccccggcc gccgcgagat cgggcacggt
     4621 gccttggccg agcgttcggt ggcctcgatt ctgcccagcc ccgctcgctt tccctatacg
     4681 atccgctgcg tctccgacat cctcgagtcc aacggctcga gctcgatggc ttcggtctgc
     4741 ggcgccacgc tcagcctgat ggacgcgggc gtgccgatca gcgacccggt cggcggcatc
     4801 tcgattggct tggtgcagga cgagtcgacg ggccgccaca tcttgttgac cgatatcatc
     4861 ggtgatgaag accacttcgg cgacatggac ttcaaggtcg ccggcacgca gcacggcgtc
     4921 accggcatcc agctcgatct caaaaaccgg ggtatcaccg aagaaattat ccgcgagacg
     4981 ctcgagcagg ctcatgaggc tcgggttgaa atcctgcgcg ccatgctgcg atcgatcaag
     5041 cggccccgcg aagagatctc cgccaacgcg ccgcggctga tccagatcca gatcaatccg
     5101 gaaaagatcg gcatggtcat cggccccgga ggcaaaacga tccgcaggct gcaggaggag
     5161 acgggtgcca agatcgacat cgatgatacc ggtgtggtga ccctctccag cctggaggcc
     5221 gccggcgccg aggccgctcg cgacaagatc gtggccatga ccgagggcgt gcaggtcggt
     5281 cgtatctacg agggccgggt gacgtctatc aaggagttcg gcgccttcgt cgagattctc
     5341 cccggcaagg acggtctggt tcatatcagc gagctgtccg atggatatgt ctcgaatgtg
     5401 accgaggtct gccgcgtggg agacccgatg ctggtcaagg cgatcgcagt cgacgatcaa
     5461 gatcgagtca agctgtcgcg taaagcggct cttgccgaac ggggtcaggt cgacgagctc
     5521 gcggggagaa cccgaccggc accgaccggg ggtgggggtg accgaggcgg acccggcgat
     5581 cgaggtggcc ccgggtctgg accgccgccg cggcgtcctc ccaacggcgg cgaccgcgat
     5641 cgtgatcgag gctacggcgg cgatcggggc ggcccgcgcg gtcccggacg tccgtgacgc
     5701 cgatggattt tgggccagac gtttcttcgt ggtttagcaa tcctggtagg gccctcgcgt
     5761 agacggaagc ggctatttta gtagtcgctc cgcgaccaga cttgcggtaa ccggcggagt
     5821 tctcgcatgg caaacttgtg gcctggcacc ggttccgagg cacagttgaa aacacggggc
     5881 gaaccgcgcg ccgcc
//
LOCUS       DATAQL010001038         6776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1784890, whole genome shotgun sequence.
ACCESSION   DATAQL010001038 DATAQL010000000
VERSION     DATAQL010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784890"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VHS97_17915"
     CDS             complement(<1..322)
                     /locus_tag="VHS97_17915"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3450136.1"
                     /translation="MGPINVLIGANGSGKSNFVSFFKMISEMANERLQVYIGQSGGAN
                     SVAHLGSKMTPRIEVELVFKTQTGFCHYKFYLVHAASDSLIFDEERLIPVVSGPKAIW
                     ISTGG"
     gene            complement(520..4071)
                     /locus_tag="VHS97_17920"
     CDS             complement(520..4071)
                     /locus_tag="VHS97_17920"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3450137.1"
                     /translation="MKLTNLHLKAVGPFTDVSLELSRGDVGLHLIYGANEAGKTSTLR
                     ALSHLLFGFPHLSADNFVHPNDQLRVGGKLRHSSGEELQIVRRRGKNSTLRGADDLAV
                     VADDCLARFLGGVNQDMFETLFGIDHQRLTQAGEEIRTGQGRLGEMLFAAGAGLAGLN
                     RAREKLRLELEALYRPKGQNPRINKTLADFRGVQDELKRQQLSSEEWQKHDHALRDSM
                     SAADEIRDHVRQARAEQSRLKRIKAAIPLAASHRRLNAELEELGNVIRLRDNFGDEFR
                     NALDQFRLAEHIITKLRTTLEELDARLAQLHPPQVLLDAASEIESLQKRLGAVEKASE
                     DRQRLENYQRDSEHQARRILRDLALTIDLDEAETLRLRVDEPVIIRGLGQRFAQLRGQ
                     AEAARNTIARHQDQIKRQEKESADLKQPRDVEPLRRAVSQARKAGDLDARLAEVRGKL
                     ARAQKKAATALAQLPDWSRSAAELEHLTIPLSATLDQFEAQLQETLRQRGSLAERIAV
                     VNDSIRQLESRLQSLELEQDVPNEEVLMTARSRREVGWQLVKSAWLDGAPAGEEHASF
                     IAEFAPKGTLATAYEQSVQRGDVLADRLRREADRVAQKAESLAQLNRHRTERVALEDE
                     ARSLNEHQARLDRDWNTLVAPLAIEAQSKTPAELRAWLHQRTEVVQLFEGVDELRQTL
                     EPLEQTFNSKHASLSRAMDELGEQLSTSDCDLAELLEKAEAVIKRHDELSQKRANLET
                     KLATARAERATSELSLGTTQAELAAWRIEWSTMMARIGLEAEATPEQAEVFLNKINEL
                     FENLTDRRSHLSRIRGIDRDAEQFAQDVMKLAARITHDPTDHPAGKRARELALRLRDA
                     QADAQRSTTLSQQRQREEASLSAAEIQRQEARICLERLCKEAGCIDFEELAEAERRSQ
                     DRARIEAARATCLDQLMEVAAGTDLATFCQQVEDADQSGLDVALEELEATIKVQEDEL
                     RRLDQTIGAERAELARMDGSDRAALTAEEAQTLLARLQGDVARYATLKLAAIVMHRGI
                     ERYREKNQGPILAHAGALFANLTCGSFARLQIDDDGDGRAVLKGVRPDGRLVNVEAMS
                     DGSHDQLYLALRLASLESWLLSHEPVPFVVDDILLNFDDQRATAALKALAELSQRTQV
                     LFFTHHHHIVDLACAHLPSEVVFVHELPDRRDSFANS"
     gene            complement(4096..5388)
                     /locus_tag="VHS97_17925"
     CDS             complement(4096..5388)
                     /locus_tag="VHS97_17925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX3450138.1"
                     /translation="MARLFTMAITFLHAADIHLDSPLKGLERYENAPVERIRGATRRA
                     FTRLVDLAIEKKVDFVLIAGDLYDGDWRDYNTGLYLVRQLGRLREQQIPVFVIAGNHD
                     AANKMTRALRLPENVRLLAHEHPETVRLEEHGVAIHGQSFGKIAVLENLAAAYPAPLK
                     GYANIGLLHTGMGGVDGHERYAPCTLEDLRARGYDYWALGHIHNRQILCKEPLIVFAG
                     NVQGRHIRETGPKGCLLVTVHSNHRIESTFQRLDHVRWERACVDVRELETDSDILGRA
                     SEVLDELLATETEPDAMLVVRVILSGPTPLHSRLQTDPERSIAEIRSLATERGRDRLW
                     IERVALDTQPVRTTTMPDGPFDELQEVIEQLRSDRSSMTTVVDELAELKRKLPAELTQ
                     DPEGPQLNDAEWLQTLLVQVQPMLLDLLLKADNGDINS"
     gene            complement(5485..6456)
                     /locus_tag="VHS97_17930"
     CDS             complement(5485..6456)
                     /locus_tag="VHS97_17930"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX3450139.1"
                     /translation="MNPRLPRVVADGPLDATIEGLFHGLVKILPWPVTMAGSDELVDG
                     LYTYGHPLVDGAMLDRLPGLKVISNYGVGVDHINLADAAARGIPVGNTPGVLEGATAD
                     LGFALLMASARRIVAGDRHAHSLDFVRYDPGYMLGREVHGTTLGIIGMGRIGRQVARR
                     AIGFDMIVLYHNRRRREDVEEAFAPSVHFAEKFDLLGRSDYVMLSLPLTNESRGCIGT
                     SELSLMKPDATLINIARGAVVDTEALTEALAQNRIAGAALDVTEPEPLPRNHPLLTMA
                     NVIITPHLGSATVETRRRMAELSVENLLAGLSGKPLPYPVSHVSGGR"
     gene            complement(6453..>6776)
                     /locus_tag="VHS97_17935"
     CDS             complement(6453..>6776)
                     /locus_tag="VHS97_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450140.1"
                     /translation="TQFYQELLTVRRENNEVFLLPAPEELIGREFAELGEMFMKYRND
                     RRSCLLIGIQRGDEMLINPIGEEAGPLHSGDQLILLSRTFPGNSQPLPISPGAASPVE
                     EKKTK"
BASE COUNT         1252 a         2040 c         2051 g         1433 t
ORIGIN      
        1 gaccccctgt gctgatccag atcgctttcg gccccgaaac aaccgggatg agccgttcct
       61 cgtcgaatat gagcgaatcc gatgcggcat gaactagata gaacttgtaa tggcaaaacc
      121 cggtttgcgt tttgaacacc agttccactt cgatgcgcgg agtcatcttc gaccctaagt
      181 gagcaaccga attagccccg ccagattgtc ctatgtagac ttgtagccgt tcgttcgcca
      241 tctcgcttat cattttgaag aacgaaacaa agttcgattt tcccgagcca ttggccccaa
      301 tcaggacatt gattgggccc aattcgagcc cggggtcgat gttccggatc gatttccatc
      361 ccgcgattcg aatgctatgt agatgtgcca taggagtgca ccgaagaccg taaggtcgag
      421 agtcctcaag cagacggaat ccatgaaacg cgggctttga ccgtaggagc cgagtctcgc
      481 atcatcttct catttcgcga cctcgtcgcc cagccgaacc taactgttgg caaaggagtc
      541 gcgccgatcc ggcagttcat ggacaaaaac gacctcagac ggcagatgtg cgcacgccag
      601 gtcgacgatg tggtgatggt gcgtgaagaa caagacctgg gttcgttgcg acagctcggc
      661 cagagccttg agagcggcgg tggcgcgctg gtcgtcgaag ttgagcaaga tgtcatcgac
      721 cacaaacggc actggctcat gcgaaagaag ccacgactcg aggcttgcaa gtcgtaaggc
      781 gaggtaaagc tgatcgtgtg agccgtcact cattgcttcg acgttcacga ggcggccgtc
      841 gggccggacc cccttcaaga ccgcgcggcc gtcgccatca tcgtcgattt gaaggcgcgc
      901 gaacgagcca caggtcaaat tggcgaaaag tgcgccggca tgagccagga tcggaccttg
      961 atttttctcg cgataccgtt cgatgccacg gtgcatcaca atcgccgcca gcttgagggt
     1021 cgcgtaccgc gcgacgtcgc cttgaagtcg ggcgagaagc gtttgtgctt cctctgcagt
     1081 tagggcagca cggtcgctac cgtccatgcg ggccaattcg gcccgctcgg cgccgatggt
     1141 ttggtccagg cgacgcaact cgtcctcctg aactttgatc gttgcttcga gctcttcgag
     1201 tgccacgtcc aggccacttt ggtcggcgtc ctcgacttgc tggcaaaacg tggcgagatc
     1261 ggtccccgcg gcgacctcca tgagctggtc gagacaggtg gcgcgtgccg cctcgatccg
     1321 ggcacggtcc tgcgagcggc gctcggcttc ggccagttct tcgaaatcaa tgcatcccgc
     1381 ttccttgcag agccgttcca ggcaaatccg agcctcctgc cgctgaattt cggcggcgct
     1441 cagactggcc tcttcgcgct gacgctgttg ggagagcgtg gtgcttcgct gtgcgtcggc
     1501 ctgcgcgtct cggaggcgta gggccagttc gcgtgcccgc tttcccgctg ggtggtcggt
     1561 aggatcatgt gtaatgcggg cggccagttt catgacgtct tgcgcgaact gttcagcgtc
     1621 gcggtcgatc ccacgaatcc ggctcagatg gctacgcctg tcggtcagat tctcaaacaa
     1681 ctcgttaatc ttgttcagaa aaacctcggc ctgctcggga gtggcctccg cttcgaggcc
     1741 aatgcgggcc atcatcgtgg accactcgat tcgccaggcc gcgagctcgg cttgggtggt
     1801 cccgagcgac agctcggagg tagcccgttc ggcacgggcc gtggcgagct tggtttcgag
     1861 gtttgcgcgt ttctgggaca gttcgtcgtg ccgtttaatc actgcctcgg ccttttccag
     1921 tagttcggcc aagtcgcagt ccgaagttga aagctgttca ccaagctcgt ccatagctcg
     1981 gctcaacgac gcgtgtttcg aattgaaggt ctgttcgagc ggttcgagcg tctgacgaag
     2041 ttcgtccacc ccttcgaaga gctgcacaac ctctgtacgc tggtgaagcc acgcgcgaag
     2101 ctcggccggc gtttttgatt gggcctcgat cgccagcgga gcgaccagcg tgttccaatc
     2161 tctgtcgagg cgagcctgat gttcattgag gctcctggct tcgtcttcga gcgccactcg
     2221 ctcagtccga tgtcgattga gctgggccag cgactcggcc ttttgcgcga cgcggtcggc
     2281 ctcacgacgc aggcggtcgg ccagtacatc accgcgctga acgctttgtt cgtaggctgt
     2341 ggcgagcgtt cccttcggag cgaactcagc aataaacgac gcgtgttctt ctccagcggg
     2401 cgcgccgtcg agccaggcgg atttgaccag ttgccagcct acttcgcgcc ggcttcgtgc
     2461 cgtcataaga acttcctcgt tgggcacgtc ttgctcgagt tccagcgatt gcaaccggga
     2521 ctcgagctga cggatcgagt catttacaac ggcgatccgc tcggcgagtg aaccgcgctg
     2581 tctcaatgtt tcctgaagct gcgcctcgaa ttgatccagg gttgcactca agggaatggt
     2641 caaatgttcg agctctgctg ccgagcggct ccagtctgga agctgggcca aggcggttgc
     2701 ggcctttttc tgggcacgcg caagtttacc tcggacttca gcgagccgcg catccaagtc
     2761 accggccttg cgagcctggc tgactgcccg acggagtggt tccacatcgc gcggttgttt
     2821 gaggtcagcc gactcttttt cctgccgctt gatctgatcc tgatggcggg cgatcgtatt
     2881 ccgcgccgcc tcggcctggc cgcgcaattg ggcgaaccgc tgtcccagcc cgcgaataat
     2941 caccggttcg tcgacccgga gccgtaatgt ttcggcttca tccagatcaa tcgtgagagc
     3001 caagtcgcga aggatccgcc gcgcctggtg ctccgagtcg cgctgataat tctccaggcg
     3061 ttggcggtcc tcactcgctt tttccactgc acccaagcgc ttctggagcg attcgatctc
     3121 gctcgcggca tccaacagca cttggggtgg gtggagctgg gccagccgcg cgtcgagttc
     3181 ttcgagcgtg gtgcgcaatt tagtgatgat atgctcggcc aaacggaatt gatcaagcgc
     3241 attgcgaaac tcgtccccga agttgtcgcg cagacggatc acatttccca gctcctcgag
     3301 ttccgcattc agtcgccggt ggctggccgc tagcggaatc gccgctttga tgcgtttgag
     3361 cctggactgt tctgcgcggg cctggcgaac gtggtcgcgg atctcatccg ccgcgctcat
     3421 cgaatcgcgg agggcatggt catgcttctg ccactcttcg ctcgacaact gttgccgctt
     3481 gagctcgtct tgaacgccgc gaaaatcagc cagagtcttg ttgatacgcg gattttggcc
     3541 tttcggtcta tagagggcct ccaactcgag ccgcagcttc tccctcgcgc gattcaagcc
     3601 cgctagacca gccccggccg caaagagcat ctcaccgaga cgcccctgcc ccgtgcggat
     3661 ttcttctccc gcctgcgtca ggcgctggtg atcgatgccg aacaacgtct caaacatgtc
     3721 ctggtttacc cctcccagga atcgagccaa gcagtcgtcg gcgacgaccg caagatcatc
     3781 ggcaccgcgg agagtgctgt tcttgccgcg acgtcggacg atctggagct cctcgccact
     3841 actatgccgc aatttccccc cgacgcgaag ctggtcattg ggatggacga agttgtccgc
     3901 cgacagatgc ggaaagccaa aaagcaggtg cgacaatgcg cgcagcgtcg aagtcttgcc
     3961 tgcctcattg gcgccataga taaggtgtag acccacgtcg cccctggaaa gttccagcga
     4021 gacgtcagtg aacggtccaa ccgccttcaa atgcagattc gtcaatttca tgttatctgc
     4081 agatcatttc gagtcctagc tatttatgtc gccattatct gctttaagca acaagtccag
     4141 aagcatcggc tggacctgga ccagcagtgt ttgcaaccat tcggcgtcgt tgagttgcgg
     4201 gccttcgggg tcttgggtta gttcggccgg caacttgcgt ttcaattcag cgagctcgtc
     4261 gacgacggta gtcatggacg aacgatcgga gcggagttgt tcgatcacct cctgcagctc
     4321 gtcaaacggg ccgtctggca ttgtggtggt ccgcaccggt tgggtatcga gcgcgaccct
     4381 ttcgatccag agccgatcgc gaccccgttc ggttgccagg ctgcggatct cggctatgga
     4441 tcgctcgggg tcagtctgca gccggctatg cagtggggtc ggtccgctga gaatcacgcg
     4501 gactaccagc atcgcatccg gctcggtctc ggttgcgagc aattcatcga gcacctcgga
     4561 cgctcggccc agaatatccg aatcggtctc gagctctcga acgtcgacgc aagctcgctc
     4621 ccagcgaacg tggtccagtc gctggaacgt ggattcgatt cggtggttgg aatgaaccgt
     4681 cactagcagg catcccttcg gcccggtctc gcggatatgc cggccttgca cattgcccgc
     4741 gaatacgatc agcggttcct tgcacaggat ttggcgattg tggatgtgac ccagagccca
     4801 gtagtcgtaa ccgcgagctc gaagatcctc gagcgtgcac ggggcatagc gctcatggcc
     4861 atccactcct cccatgcccg tatggagcag cccgatgttg gcatagccct tcaggggcgc
     4921 cgggtaggcc gcggccagat tctcaaggac ggcgattttc ccaaaacttt gcccatggat
     4981 ggcgacaccg tgctcctcaa gccgaaccgt ttccgggtgt tcgtgagcca gtaatcgtac
     5041 attttcgggc agccgcagag ctcgagtcat tttgttggcg gcgtcgtggt tacctgcgat
     5101 cacgaagacc gggatctgct gctcgcgcag ccggccaagt tgacggacga gatacaagcc
     5161 ggtgttgtaa tcgcgccaat caccgtcata aaggtcgccg gcgatcaaga cgaaatcgac
     5221 ctttttctcg atggccaagt cgaccaacct ggtgaaggcg cgtcgcgttg ctccgcgtat
     5281 ccgctcgacg ggtgcgttct cgtatcgctc aagacccttc agagggctat cgagatgaat
     5341 atccgcagcg tgcagaaatg tgatcgccat ggtgaagagc cgcgccactc ggccacacat
     5401 ccaggagaca gcacgctgct gtaacccgcc cggggtggag tatacgattc tacagtatcc
     5461 ggccatcggg gtgcgagagg cgattcaacg tccgccggag acatgggaga ccgggtacgg
     5521 tagcggcttg ccgctgaggc cagccaagag gttctcgacg gagagttcgg ccatgcggcg
     5581 gcgggtctca accgtcgcac tcccaaggtg gggcgtgatg atgacgtttg ccatggttag
     5641 aagggggtgg tttcggggca gtggctcggg ctcggtcacg tcgagggccg cgccggcgat
     5701 ccggttttgt gccaacgcct ccgtcagcgc ctcggtgtcg accactgcgc cgcgagcgat
     5761 gttgatcagc gtggcgtcgg gcttcatcag cgagagctcg cttgtgccga tgcagccgcg
     5821 ggactcgttg gtcagcggta aagagagcat cacataatcg gatcgaccca acaggtcaaa
     5881 cttctcggcg aagtgtaccg acggggcgaa agcctcttca acgtcttcgc gccgtctgcg
     5941 gttgtggtag agcacgatca tgtcgaagcc gatcgcccgc cgggcgacct ggcggccgat
     6001 ccggcccatc ccgatgattc ccaacgtcgt gccgtggacc tcgcggccca gcatataccc
     6061 agggtcatag cgaacgaaat cgagcgagtg ggcgtggcgg tcccctgcga cgatccgccg
     6121 cgccgaggcc atcaagagcg caaagcccag gtcggccgtc gccccctcga gcacgccggg
     6181 cgtgttacca accgggatgc cccgagccgc cgcatccgcc aggttgatgt ggtcgacgcc
     6241 tacgccgtag ttactgatca cctttagacc ggggagcctg tcgagcatgg caccgtcgac
     6301 caggggatgg ccgtaagtgt acagcccatc cacgagctcg tcgctccccg ccatggtcac
     6361 tggccagggg aggattttga cgaggccatg gaacaggccc tcgattgtcg cgtccaacgg
     6421 cccgtccgct acgacgcggg gaagtcgcgg gttcacttcg tttttttctc ctcgacaggc
     6481 gatgcggcgc cagggctgat cgggagcggc tgagaattgc caggaaaagt tcggctcaag
     6541 aggataagct ggtcgccgga atgcagcggg cccgcttctt cgccaatagg attgatcagc
     6601 atctcgtcgc cgcgctgaat gccgatcaac aggcaggacc ggcgatcgtt gcgatacttc
     6661 atgaacatct cgccgagctc ggcgaattcg cggccaatca gttcctccgg cgccggcagc
     6721 aagaacacct cgttgttttc acggcggacg gtgagaagct cttggtagaa ctgagt
//
LOCUS       DATAQL010001039         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4531384, whole genome shotgun sequence.
ACCESSION   DATAQL010001039 DATAQL010000000
VERSION     DATAQL010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4531384"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..778
                     /locus_tag="VHS97_17940"
     CDS             104..778
                     /locus_tag="VHS97_17940"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3450141.1"
                     /translation="MVEGLEKLRREKKIDAKNPDSPLALTLHPQQALWRLMLGGVFDR
                     FPTLQLVLTEVRADWVPATLKYLDQRFAEHPGKCELKPSEYYERNCALTPSAPHRAEV
                     AMLDQIGVSHVMFGADIPNPEATWPNSRQWIADAFRGVPEDKLRMVLGENAIRVYRLD
                     RAALAKTAARLAVRPSDLFTGDALDDRLLDHFDARAGYRRPADEIDEDLIGGELESDF
                     ARLVNN"
     gene            complement(960..1544)
                     /locus_tag="VHS97_17945"
     CDS             complement(960..1544)
                     /locus_tag="VHS97_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450142.1"
                     /translation="MALWYFFGARYTENYTAEIDGFLHRLDDLGLLGMDECANLNEAR
                     AHGNPYLDTSSMIRQASTAVMGGQMKTYDSEDQVRAAGEEAPSGHGYQEMFEVLPEIW
                     DQNHGFHIIDADDPELLEHGEAYIHERLTSYVAEPFREGSEVALPPYIRPYVERNTVN
                     ALAIGWYAGLPAVFLEPLRQRLGFPYEWWGAPID"
BASE COUNT          328 a          527 c          586 g          347 t
ORIGIN      
        1 gccccagggc accgcgatgc aattcatgcg ccagcttgcc cccggcaagg gcatgaacat
       61 gatgcaaccc gagtcggggt cgtcggcgga ggggagtctc gaggtggtcg aggggctgga
      121 gaagctccgt cgcgagaaga agatcgatgc aaagaacccc gattcgccgt tggcgttgac
      181 cctgcacccg caacaggcgt tgtggcggct gatgcttggc ggcgtttttg atcggttccc
      241 gaccttgcag ctggtgttga cggaggtccg cgcggattgg gtgccggcga cgttgaagta
      301 tttggatcag cggttcgccg agcatccggg caagtgtgaa ctgaaaccca gtgagtacta
      361 cgagcgaaac tgcgcgctca caccgtcggc gccgcatcgc gccgaagtgg caatgctgga
      421 tcagatcggg gtttcccatg tcatgttcgg cgccgacatc cctaacccgg aggccacctg
      481 gcccaactct aggcagtgga tcgctgatgc gtttcgcggc gtccccgaag acaaactacg
      541 aatggtgctg ggcgagaacg cgattcgggt ctaccggctg gatcgcgcgg cgctggccaa
      601 gaccgcggcg cgactagcgg tgaggcccag cgatttgttc accggggatg cccttgacga
      661 tcgtcttctg gaccatttcg acgctagggc gggctaccgg cgtccagccg acgagatcga
      721 cgaggatcta atcggcggtg agctggaatc ggacttcgct cgactcgtga acaactgacc
      781 tttctccaaa gcctgaaggc actcgcagtc cgtgccggaa cctttgacaa agccacgtgt
      841 cacggattgt tcgcggaatc ctgaacaggc agcggtcggg caggatttcg cagtcccgtg
      901 tgcaccgtgc ccgggaacca cggtcggcga cgattgacgc tcggagtgtt ccggtctggt
      961 caatcgatcg gcgcgcccca ccactcatag gggaacccca ggcgctgacg cagtggctcg
     1021 aggaagacgg cgggtagccc cgcgtaccac ccgatcgcga gggcgttgac cgtgttgcgc
     1081 tcgacatagg gccggatata ggggggcagc gccacctccg agccctcacg gaacggttcg
     1141 gctacatagc ttgtgagtcg ctcgtggatg taggcctcgc cgtgctcgag tagctcggga
     1201 tcgtcggcat cgatgatgtg gaagccatgg ttctggtccc agatttccgg caggacctcg
     1261 aacatctcct gatagccgtg ccccgagggc gcctcctcgc ctgcggcacg gacctgatct
     1321 tcggaatcgt acgtcttcat ctgccccccc atgacggcgg tggacgcctg acggatcatc
     1381 gatgaggtat cgaggtaggg gttgccatgc gctcgggcct cattgaggtt ggcgcactcg
     1441 tccatcccca agagtcctaa gtcgtctagc cgatgcagga agccgtcgat ttcggcggtg
     1501 tagttttcgg tgtacctagc gccaaagaaa taccacagag ccacgggttt ccgacctcct
     1561 tatgtcgtgt cggaaggtta gcatgcgtga acctttccga tgagcgtgac cggaactgcg
     1621 cttcgtaaag ccagacggcg cgacgagggt aactggatgt tcgacttcta tggtgtccat
     1681 ggaagtcggc cgacccgtgg cagcgtggcg atcaccgccg ctgcgcgccc gtcgacgttc
     1741 gacggcgagt tgcgctcagc aggtcttttg catgcagggc gccgcaat
//
LOCUS       DATAQL010001040         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1021436, whole genome shotgun sequence.
ACCESSION   DATAQL010001040 DATAQL010000000
VERSION     DATAQL010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021436"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VHS97_17950"
     CDS             complement(<1..364)
                     /locus_tag="VHS97_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450143.1"
                     /translation="MTARRFSRRRMLEAGTAGIAGLSLPSLLKAEQKPGLQARAKHII
                     FLHQFGGPSHLDTFDMKPAAPSGIRGEFQPIATREPGVFVSEYMPRFATIVDQFAQIR
                     SVNHRMKNHNSATYYSLTG"
     gene            complement(407..>1697)
                     /locus_tag="VHS97_17955"
     CDS             complement(407..>1697)
                     /locus_tag="VHS97_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3450144.1"
                     /translation="ADFWALKWADLLRNEEKTMGEKGAWVFQRWLRDELARDVPLDSL
                     VRRIVAGLGSTWQNPPSSFHRTNRDPMTAAESVGQVFLGVRLQCARCHNHPFDVWTQD
                     DYYGLAAFFGNIARKQPSNVRSDRLDSHEINGDEYIYLSGPPQMVQPRSGVLLKPKAL
                     TGMAARGFDGDRENALLHLADWLTRQNHQFSRNLANLVWFHLLSRGIVDPVDDFRDSN
                     PPSNPALLDLVTAQFEAGGMRLKPLVAWIMKSGIYQLSATPNSTNAEDQVNFSHAAVR
                     LLPAEVLLDAVSQVLDVPEQFRHAPASLRAAQLPGASGGVAFLKTFGKPDRLLTCECE
                     RSESTTLAQAFQMINGETVRKKLEMDSNRIGHCLNAGRPDSQILDEVYLAAVARWPTA
                     TERVAVTTHLARSSEKRKAWEDVVWALLNSKEFLLRH"
BASE COUNT          334 a          491 c          533 g          339 t
ORIGIN      
        1 gccctgtcag gctgtagtag gtcgccgagt tgtggttttt catccggtga ttgaccgacc
       61 ggatctgggc aaattgatca acgattgtgg cgaatcgagg catgtattcg ctgacaaaca
      121 cgcccggctc gcgagtcgcg atcggctgaa actccccgcg aatcccgctg ggagccgccg
      181 gtttcatgtc aaacgtgtcc agatgcgacg ggccgccaaa ctggtgcaag aagatgatgt
      241 gtttcgcacg tgcctgcaag cccggctttt gctcggcctt gagcaatgaa ggaaggctca
      301 agccggcgat gcccgcggtg cccgcttcga gcatccggcg ccgcgagaag cgcctcgcgg
      361 tcatgaaatc ggggcaaaac ggtaacatgc tttaaagccc cccgagtcag tgtcgcagca
      421 aaaactcttt actgttcaat agtgcccaga cgacgtcttc ccatgccttg cgcttttcgc
      481 tggagcgcgc tagatgcgtg gttacggcca ctcgctcggt cgcggtcggc cagcgcgcca
      541 ccgcggccag atagacttcg tccaggatct gggaatccgg ccggcccgca ttcaggcagt
      601 ggccgatcct gttggagtcc atttccaact tcttgcgcac ggtttcgccg ttgatcatct
      661 ggaatgcctg agccagggtg gtcgactcgg accgttcgca ctcgcatgtc aaaagccggt
      721 cgggcttgcc gaatgtcttt aaaaacgcga caccgcccga tgcgccgggg agttgggcgg
      781 cgcggagcga cgctggtgcg tgccgaaatt gttcgggtac gtccaggacc tgactgacgg
      841 catcgagcag cacctcggcg ggaagcaatc gaaccgccgc gtgcgagaaa ttcacttgat
      901 cttcggcatt ggtcgagttc ggagtcgcgc tcaactggta gatgcccgac ttcatgatcc
      961 acgcaaccaa gggcttgagc cgcataccgc cggcctcgaa ctgggctgtc accaggtcga
     1021 ggagggccgg attggaggga ggattcgagt cgcggaagtc gtcgacagga tcgacgatgc
     1081 ctctgcttag caagtgaaac cagaccagat tggcaaggtt acggctgaat tgatggtttt
     1141 gccgcgtcag ccagtccgcc agatgtagaa gcgcgttttc gcggtcgccg tcgaatcctc
     1201 gggcggccat cccggtgaga gcctttggct tcaaaagcac accggaacgg ggctggacca
     1261 tctgaggagg tcccgacaaa taaatatatt cgtcgccgtt gatctcgtgt gaatcgagtc
     1321 ggtcactgcg tacattgctg ggctgcttgc gggcgatgtt gccgaaaaag gcggccaggc
     1381 cgtagtagtc gtcttgagtc cagacgtcga aagggtggtt atgacaacgt gcacactgca
     1441 ggcgaacacc caggaagacc tggcccacac tctccgcggc ggtcatggga tcgcggttgg
     1501 tccgatggaa gctcgacggc ggattctgcc aggtcgagcc caggccggcg acgatccgcc
     1561 gcaccaagct gtcgagtgga acgtcgcggg cgagttcgtc gcgcagccag cgctgaaaga
     1621 cccaggcccc tttttcgccc atggtcttct cttcgttgcg aagcaaatcg gcccatttga
     1681 gcgcccagaa atcggcg
//
LOCUS       DATAQL010001041         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4073751, whole genome shotgun sequence.
ACCESSION   DATAQL010001041 DATAQL010000000
VERSION     DATAQL010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4073751"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VHS97_17960"
     CDS             <1..722
                     /locus_tag="VHS97_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3450145.1"
                     /translation="CAVNCIELAVERGTFYGFLGPNGAGKSTTIKMLTGLLAPTSGQM
                     LVLGRNMLDPHEALEAKRRIGVIPEDLALFDNLTAREYLTFVGRIHLMPRETIRNRCD
                     ELLAVLDLHGEEKKLTVEYSHGMKKKLAMAAALLPNPDLLFLDEPFEGVDAVTSRVIR
                     DLLAAFVARGSTVFLTSHVLEIVERLCTDVGIIVKGELVEQSSLDTIRLGGSLEDRFL
                     QKAGADHEATRKLNWLEEAAS"
     gene            719..2569
                     /locus_tag="VHS97_17965"
     CDS             719..2569
                     /locus_tag="VHS97_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450146.1"
                     /translation="MNWQQLGAVVWLRWRLLVNQWRKAGAFTVALMVIVTVGALVTAI
                     PLFIGSFMLGQYAIPKAAPAHLMYVWDGLIVAFLFFWSVGVVAELQRTEPLSLVKFMH
                     LPVSITGAFLINYLSSLLRLSLIFFGPIMLGFSLALVFVKGIVFAPVFLLLAAFFLMV
                     TALTYQLQGWLASLMSNPRRRRTVVVATTAIFVLIVQLPNLINFFAPWGPRQQLVRSA
                     KFQDETAKIKHAYEAGEFDAHEFLRRTQEAMEAQKHAMQQEDRASLKEWEQKADLANV
                     ILPVGWLPLGVKAAAEGRIMPSMLGLLGMGLIGIASLYRAYGTTIDMYQGQSSNPRGR
                     PTPVPAVAQPAAARKARTLLLESRLPGLSEPTSAIALSGLRSLVRSPEAKMMLLTPVI
                     MIPIFGSMLWRGRDNIPESVRPLVAFGGMLFVLLGMVQMMTNQFGFDRDGFRVFVLSP
                     ASRRDILLGKNLAFAPMALGMAAIVLVVVQMLCPMRLDHFLTMLPQYLSMFPVFCILS
                     NLVSIYAPAHVAAGSLRPANPKLTTILLQTVMIGMIFPLTQAVILMPLGIEFLLKYFG
                     WGGGVPIFLVLSIVECALVVLIYYLSLIWQGQLLQGREQRILECVTNRAA"
     gene            complement(2575..3600)
                     /locus_tag="VHS97_17970"
     CDS             complement(2575..3600)
                     /locus_tag="VHS97_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3450147.1"
                     /translation="MNRERLDELANRYRQLRIGVVGDFFLDRYLHIDAAKAEKSIETG
                     LPVHNVVAVRSQPGAAGTILNNLVALEVGAIHAVGYCGDDGEGYELRRALARLRGVSL
                     ENLVTSSSRLTPVYCKPLLFEPGRLPRELNRLDTKNWSPTPGELERDLADRVGKLAPS
                     IDVLLLLDQVEIAETGVATRLVCEAVYAAMREHKGLRVLADSRQGLHHFPPVGFKMNA
                     AELGRMTQSSSNELEAVKARAAVLANSTGQPVFVTLAERGIVGAFPGHNPDYVPAYPV
                     RGPIDVVGAGDAVTANLATALAAGASPREAMELAMAAASLVIHQLDTTGTASIRQIGS
                     LLAQDTS"
     gene            complement(3597..4697)
                     /locus_tag="VHS97_17975"
     CDS             complement(3597..4697)
                     /locus_tag="VHS97_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450148.1"
                     /translation="MQPLDLSQLKVLPLSKRESLTRVDELLIEPDAPLQPCTEKVASS
                     ISDCAQKIMAARAKGATVILIYGAHLLRNGAAKILERMMSAGWLSHLATNGAGTIHDW
                     EYAWLGASTESVEKNVASGTFGSWHETATNIHLALLSGALDGLGYGRSLGRFIHEDGA
                     TIPDAEELAAAIAAAPQHPLASARADLLRTMSEQHWPAGRIRVAHRWKHASIVAQAYR
                     HGVPFTVHPGVGYDIISNHPVFCGAAIGRGAELDFKLFGGSLQDLDDGVVLSVGSAIM
                     GPQVFEKALSCVNNLRLQAGRDVVRGHTFYVVDLQDGGGWDWTRGEPPKTNPAYYLRF
                     CKSFSRMGGALNYVQCDNATFIQHLHRRLSET"
     gene            complement(4749..>5322)
                     /locus_tag="VHS97_17980"
     CDS             complement(4749..>5322)
                     /locus_tag="VHS97_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3450149.1"
                     /translation="ERIEERGGQPQEPLWYKHEYLRRLEAHIGARTAGLKSGALAADS
                     LLVHGARPLLEHLHDCGLHLYLASGTDESAVKQEATLLGITGYFGPNIYGALDDYKQF
                     SKKMIIDRILNEHDIMGRQLLSFGDGYVEIENTKQVGGLAVAVASDEAHNGSGRVDQW
                     KRARLLSVGADAVIPDFRDAIPFVDFVLGR"
BASE COUNT          960 a         1515 c         1692 g         1155 t
ORIGIN      
        1 catgcgcggt caactgcata gaactggcgg tcgagcgcgg aaccttttat ggatttctgg
       61 ggcccaatgg ggccggcaag tcgacgacga tcaagatgct cactggtctt ctcgcgccga
      121 caagcgggca aatgctcgta ctgggacgga atatgctcga tccgcacgag gcgctcgagg
      181 caaagcggcg gattggggtc attcccgaag acctggcgct gtttgacaac ctgacggcgc
      241 gcgaatatct cacattcgtc ggccgcatcc atctgatgcc gcgggagacg atccgcaacc
      301 gttgcgatga gctccttgcc gtgctcgatt tgcatggcga agaaaagaag ctcacggtgg
      361 aatactcgca cggaatgaag aaaaaactgg ccatggccgc ggctctgttg ccgaatccgg
      421 atctgctgtt tctcgacgag ccgttcgagg gtgtcgatgc ggtcacgtcg cgcgtgatcc
      481 gcgatttgct cgcggcgttt gtcgcccgcg gatccacggt ctttctcact tcgcacgtgc
      541 tcgagatcgt ggaacgcctc tgtacggatg tcggcatcat cgtgaaaggc gagctggtcg
      601 agcagtcttc gctcgacaca attcgccttg gaggctcact ggaggatcgt tttcttcaaa
      661 aagcgggtgc cgatcacgaa gcgacgcgca agctcaattg gctcgaggag gcggcgtcgt
      721 gaactggcag cagttggggg ctgtcgtatg gctgcggtgg cggctcctgg tcaaccagtg
      781 gcgtaaggcc ggcgcgttca ccgtggccct catggtgatc gtgaccgtcg gcgcgctggt
      841 gacggctata ccacttttca ttggctcgtt catgctcggt caatatgcga ttcccaaagc
      901 cgcgccggcg catctgatgt atgtctggga cgggctgatc gtcgcgtttc tgtttttttg
      961 gagtgtgggc gtggtcgccg agctccagag gaccgagccc ttgtcgctgg tgaagttcat
     1021 gcacctgccc gtgtcgatca ccggcgcgtt tctgatcaat tatctcagct cgctcttgcg
     1081 tctctccctc atcttcttcg ggccgatcat gctgggattt tcgctggcgc tcgtgttcgt
     1141 caagggaatc gtgttcgcgc ccgtgttcct gttgttggcg gctttcttct tgatggtgac
     1201 ggcgctcacc tatcagctcc aaggctggct ggcctcattg atgagcaatc ctcgccggcg
     1261 ccggactgtg gtggtggcca cgacagcgat ctttgtattg atcgttcagc taccgaatct
     1321 gatcaacttc ttcgcgccct ggggcccgcg ccagcagctc gtccggtcgg cgaagttcca
     1381 ggacgaaaca gccaagatca agcatgcgta tgaagcagga gaattcgatg ctcatgagtt
     1441 tttgcgtcgg acgcaagaag ccatggaagc gcaaaagcat gccatgcagc aggaggatcg
     1501 cgcgagcttg aaagagtggg aacagaaggc cgatctggcg aacgtgattc tgcccgtcgg
     1561 ctggctccct ttgggcgtga aggctgcggc ggagggccgg atcatgccgt cgatgctggg
     1621 attattggga atgggactga ttggaattgc gagtctctat cgggcgtacg gcacgacgat
     1681 cgatatgtat cagggccaat cgagtaaccc taggggccga ccaactcccg tgcctgccgt
     1741 ggcgcagcct gcggccgcgc ggaaggcacg aacgctcttg ctcgagtcgc gattgccagg
     1801 cttgtcggaa ccgacttccg ccatcgcgct ctcgggcttg cgttcgctcg tgcgatcgcc
     1861 cgaggcaaag atgatgttgt tgaccccggt catcatgatt cccatttttg gctcgatgct
     1921 ctggaggggt cgcgacaata ttccggagtc ggttcggccg ctggtcgctt ttggtgggat
     1981 gctctttgtc ctcctgggca tggtgcagat gatgaccaat cagttcggat tcgaccgcga
     2041 cggctttcgc gtgttcgtgc tgagcccggc atcgcggcgc gatatcttgc tgggcaagaa
     2101 tctggcgttc gctcccatgg ctttgggaat ggcggcgatc gtgctggtcg tcgtgcagat
     2161 gctttgcccc atgcgattgg accattttct gacgatgttg cctcagtatc tttcgatgtt
     2221 tccggtgttc tgcatcctgt caaacctggt gtcgatctat gcgccggcac acgtcgcggc
     2281 cggctcgctt cggccggcga atcctaaact aaccacgatc cttttgcaaa cggtaatgat
     2341 tggcatgatt tttccgttga cccaggctgt gatattgatg ccgctgggca tcgagttcct
     2401 gctcaagtat ttcggttggg gcggtggcgt gccgatcttt ttggtgctat cgatagtgga
     2461 atgcgcactg gttgtgctca tatattatct gtccctcatc tggcaagggc agctgttgca
     2521 agggcgcgag cagcggatcc tggaatgcgt gaccaatcga gcggcttgaa ttgatcagct
     2581 cgtgtcctgc gccagcaagg agccgatctg gcgaatggat gccgtgccgg tagtgtcgag
     2641 ctgatggatc acgagtgacg cggcggccat ggcgagctcc attgcctcgc gcgggcttgc
     2701 tccggccgcc agggcggtag ccaggttggc agtgaccgcg tcgccggcac cgacgacgtc
     2761 gattgggccc cgcaccgggt aggccggcac ataatcgggg ttgtggccag gaaatgctcc
     2821 cacgattccg cgctcggcca aggtgacaaa gacaggctga cctgtgctgt tggcaagaac
     2881 ggcggcccgg gctttgacgg cttcgagctc gttcgagctc gattgcgtca tccgaccgag
     2941 ctcggcggcg ttcatcttga aaccgactgg cgggaaatga tggaggccct ggcggctatc
     3001 ggcgaggacc ctgaggccct tgtgttcccg catggccgcg tagaccgctt cgcagaccaa
     3061 gcgcgtggcc acaccggttt cggcgatctc gacctggtct agcaagagca agacgtcgat
     3121 cgagggggcc agcttgccga cccggtccgc cagatcgcgc tccagctcgc ccggtgtcgg
     3181 cgaccagttc ttggtgtcga gacggttcaa ttcgcgtggc aggcgccccg gctcgaacaa
     3241 gagtggtttg caatagaccg gcgtcagcct cgaggacgat gtgaccaggt tttcgaggct
     3301 cactccccgc aacctggcga gcgcacggcg cagttcatag ccttcgccgt cgtcgccgca
     3361 atagccgacc gcatgaatcg cgccaacttc gagtgcgacc agattgttaa ggatggtgcc
     3421 ggcagccccc ggctggctcc gaacggcgac gacgttgtga acgggcaggc cggtctcgat
     3481 cgacttctcc gccttggcgg catcgatgtg gagatagcgg tcgagaaaga aatcgcccac
     3541 cacgccgatg cgcaactggc gataccggtt ggcgagctcg tctagtcgct ctctgttcat
     3601 gtctcgctta accggcggtg aaggtgctgg atgaaggtcg cgttgtcgca ctgcacgtag
     3661 ttaagcgcgc cgcccatccg ggagaagctt ttgcagaaac ggaggtagta ggcggggttg
     3721 gtttttggag gctcgcctct ggtccaatcc caaccaccgc catcctggag gtcgaccaca
     3781 taaaaggtgt ggccgcgaac gacgtctcgc ccggcttgca gccgaagatt attgacgcaa
     3841 ctgagcgctt tctcgaagac ttgtgggccc atgatggccg agccgaccga gagaacaacg
     3901 ccgtcgtcaa gatcttgcaa tgaaccgccg aagagcttga aatccagctc agcgccccgg
     3961 ccgatggcgg cgccgcaaaa gacaggatgg ttggagatga tgtcgtatcc cacgccagga
     4021 tggacggtaa atgggacacc gtgacgatac gcctgggcca cgatcgacgc gtgcttccag
     4081 cggtgcgcta cgcgtatccg gccggccggc cagtgctgct cgctcatggt ccgcagcagg
     4141 tcggcacgcg cggaggcgag cggatgttgg ggtgctgcgg cgatcgccgc ggcaagttct
     4201 tcggcgtcgg gaatcgttgc accgtcttca tggatgaagc ggcccagcga tcgaccgtag
     4261 cctagaccgt cgagcgctcc ggataagagc gccagatgga tattggtcgc cgtctcatgc
     4321 caggacccga acgttcccga agccacgttt ttttccacgc tttcggtcga ggcgccgagc
     4381 caggcgtact cccagtcatg gatcgtgccg gcgccattgg ttgccaggtg agagagccag
     4441 ccggcggaca tcattcgctc gaggatcttg gcggcgccat tgcgcagcag gtgggcacca
     4501 tatatcaaga ttaccgtcgc acctttggcc cgggcggcca tgatcttttg ggcgcagtcg
     4561 gagatcgacg acgcgacttt ctcggtgcac ggctggagtg gagcgtcagg ttcgatcaag
     4621 agctcgtcga cgcgcgtaag gctctcgcgc ttcgacagcg gaaggacctt gagctgggaa
     4681 aggtcaagtg gttgcacgct caacttgagt aacctttgga tccggggcga tggtctgcgc
     4741 cgtcggcttt agcggcccag cacgaaatcg acgaacggga tggcgtcgcg gaagtcgggg
     4801 ataacggcat cggcacccac acttagcaac cgcgcgcgtt tccactggtc aactcgaccc
     4861 gagccgttgt gggcttcgtc agacgccacg gccaccgcca ggccgccgac ctgcttggtg
     4921 ttttcgatct cgacgtagcc atcgccaaac gaaagtaatt ggcggcccat gatgtcgtgt
     4981 tcgttcagga ttctgtcgat aatcatcttc ttcgaaaact gcttgtagtc gtcgagagct
     5041 ccataaatgt tgggaccgaa atagccggta atgcccagga gggtggcctc ttgtttaacg
     5101 gccgattcgt cggttccgct ggctagataa agatgaagtc cgcaatcatg caagtgttcc
     5161 aaaagcgggc gcgccccatg tactaaaagg ctgtcggccg caagcgcgcc actcttgaga
     5221 cctgccgtac gtgcgccgat atgcgcttcg agccggcgta gatattcgtg tttgtaccag
     5281 agcggctctt gaggctggcc gcctctctct tcgatccgct cg
//
LOCUS       DATAQL010001042         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3922827, whole genome shotgun sequence.
ACCESSION   DATAQL010001042 DATAQL010000000
VERSION     DATAQL010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3922827"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1454
                     /locus_tag="VHS97_17985"
     CDS             183..1454
                     /locus_tag="VHS97_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX3450150.1"
                     /translation="MSRSEARGRVYLSMDWMMHASVNFQDERLDFELPDERVVANWNA
                     PAGLDPRQAVEALRDALETPWDFPPLRQMIVPGDRVVIALDSSITHAGPILKVLGQTL
                     RDAGVESDGVTVLTPAVASARKDSATMPGNLLVVHDSRDRSQLAYLAATKEGRRIYLN
                     RLLTDADVVIPVGRLGYDPNLGHRGPWSVIFPDLSERETIEAYRGRVPNEAKGHPGAH
                     ARANLDESFEVSWLLGSQFHLGVVPGFVGPAEFVAGRENAVRERGIAALDRYWKFEAD
                     SRAELVVVGIGGPGNRATIDDLAEGVARACALVQHGGKIVALSRAEGSMGPSLQSLIA
                     TEDPKQRAAALRGHEGDDDFLTAHRLARALAWADLFVLSGLDPDVLDDLSIVALDNPE
                     QARRLVAKSGSCSFVSQAERTLAFVSSENES"
     gene            1459..>1750
                     /locus_tag="VHS97_17990"
     CDS             1459..>1750
                     /locus_tag="VHS97_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX3450151.1"
                     /translation="MITKNEFEVMATHVGLVDRSDRVRLDINGPDRAKFLHNLTTNEI
                     KRMAVGSGCEAFITSPQGKTIGYMIILAADDSIMARADPEGAELALAHFRKYG"
BASE COUNT          340 a          520 c          564 g          326 t
ORIGIN      
        1 tttgggcgat tgaaactatt cacgtttgtt cgcgctcggt cgaccgctat tcgctcggaa
       61 aatcgattcg gccggaacaa atgggcgaac cggagccggc ctcaccctgc ttgtcccgat
      121 cccgcggtca acctatcata aactggttcc aagccagatc ggccgaggtg ttctgcgcgc
      181 gaatgagtcg atccgaagcg cgtgggcggg tctacctctc gatggactgg atgatgcatg
      241 catcggtcaa ttttcaggac gaacggcttg acttcgagct accagacgag cgcgtcgtcg
      301 cgaattggaa cgcgccggcc ggactggacc cacggcaggc agtcgaagcg ctacgggatg
      361 ccctggaaac gccctgggat ttccctccac tccgccagat gatcgtgccg ggagatcgag
      421 tcgtcatcgc gttggactcg tcgatcacgc acgctgggcc gattctgaaa gtactagggc
      481 aaactctccg ggatgcgggc gtcgagtccg acggagtgac cgtgcttacg cctgccgtcg
      541 cgagcgcccg caaagacagt gcgacaatgc cgggcaactt gctggtcgtt catgattcgc
      601 gcgatcgcag ccagcttgcg tatctcgccg cgaccaagga gggccggcgc atttatctga
      661 accggctttt gaccgacgcc gacgtggtga ttcccgtggg acggctcggc tacgacccga
      721 acttgggcca tcgagggccg tggagtgtta tcttccccga tttgagcgaa cgcgaaacga
      781 tcgaggctta tcgcggacgg gtgccgaacg aggcgaaagg gcatcctgga gcgcatgccc
      841 gcgccaatct ggacgaatca ttcgaggtga gctggctact cgggagccaa ttccacttgg
      901 gggtcgtgcc gggctttgtc gggcctgcgg agtttgtagc cggccgggaa aacgcggtcc
      961 gtgaacgagg aatcgcagcg ctcgatcggt actggaagtt cgaagcggat tcgcgcgccg
     1021 agcttgttgt agtcggaatc ggcggcccag ggaacagagc gaccatcgac gacctcgccg
     1081 agggcgttgc tcgggcgtgc gccttggtgc agcatggcgg caagatcgtg gcgttatcgc
     1141 gagccgaggg gagcatgggc ccgtcgctcc agagcctgat tgccaccgaa gacccgaagc
     1201 agagagcggc cgccctccgc ggtcacgaag gggacgatga tttcctgact gcccaccgtc
     1261 tggcacgggc actcgcatgg gccgaccttt tcgttctgag cggactcgac ccggacgtgc
     1321 tcgatgacct gtcgattgtc gcgctcgaca atcccgagca agcccgccgc ctggtcgcca
     1381 agagcggctc gtgctcgttc gtcagccagg ccgagcgcac cttggccttc gtgagctcag
     1441 agaacgagag ctaacgcgat gataaccaaa aatgaatttg aagtgatggc aactcatgtc
     1501 ggtcttgtcg accggtcgga tcgcgtccgg ctcgatatca acgggccgga tcgggccaaa
     1561 ttcctccaca atctgacgac caacgagatc aagcgcatgg cggttggcag cggctgcgaa
     1621 gcgttcatca cgagccccca gggtaagacc atcggctaca tgattattct cgcagccgac
     1681 gacagcatca tggcgcgggc ggacccggag ggtgccgagc tcgctttagc acatttccgc
     1741 aagtacggcg
//
LOCUS       DATAQL010001043         5893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2089885, whole genome shotgun sequence.
ACCESSION   DATAQL010001043 DATAQL010000000
VERSION     DATAQL010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089885"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="VHS97_17995"
     CDS             complement(<1..1987)
                     /locus_tag="VHS97_17995"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX3450152.1"
                     /translation="MKRLLLFAISHLVAGLVAMNPGLVRADDKSTMIRRDEFGVPHIF
                     APSLEHAAYAAGYAQAEDRLEELLKNYRRAAGTMAEVFGADHFDADLRQRVMRHAAVS
                     QAKYNEISPKMRGVIEQYQEGIKQYMKEHPEQVPSWVGPIQPWQAVALGRYIIWNWPI
                     GEAAADLKRAGVEFGPLSYRGSNEMLIAPKRTAINAPIAIIDPHVPWYDAMRFYEVRI
                     YSPEFNVAGVSILGVPLPTLGHSRYCSIAMTTGGPDTSDIFEEVLNPANPDQYRYEGE
                     WRDFTIRKETINVKNGDRVEPRDVTLTFSHHGPIVARKSGKAYAMAIPYADEVGLTDQ
                     CYEMMTAKNLTEMKQALSHCQLMAQNIMVGTVQGDIYYLRNGRVPIRPKGVDPSRPIP
                     GDTSTTEWQGIHPISDLVQIENPPCGWMQNCNCSPAAMMNSDQPLHEHYAQNPHIYNA
                     SPSGDAHQRAEMVTDLLDAACKVTVEQAIDIAFSTQVWHAERWQARLKEAWQKAADAD
                     KTADIKEAYSLIHEWDRQSAPDSKGALAFYAFKKELSGDLAHKTEPPGDVSDAQLLHA
                     LRQGAAWAKANFGSVAVPFGEYFRVGRRGSDRSWPVGGGSLQDVGMATPRAISFAPTA
                     DRKRMIGHTGQSSTQVVILTDLPESYAIIPLGESDHLD"
     gene            complement(2100..2960)
                     /locus_tag="VHS97_18000"
     CDS             complement(2100..2960)
                     /locus_tag="VHS97_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX3450153.1"
                     /translation="MNWQTTFAGVIIAIAVGAGHEQSAGAEKSDDSKRLVLVELYTSQ
                     GCDMCPEAEKLLGALAEREGKIVPIAFHVDYFNNPWRDVFSDHLYSQRQMNYNELYTK
                     PKNPEYGLYYTPMMMIDGQQSVNGRDAASAEPAIRKALLRKPAVSLDTKLDLKSNGLA
                     GTAEIKVTSRSAQAEKTPLLVCAVLREDNVVTEIGSGENAGKALVARFPARLTKYDFI
                     ELDGSSPATQRFTFSIEPGWKRQNLRLAIFVQNKRTGEVYQAADVPWQSERAAGGAAK
                     STTATPVGPR"
     gene            complement(3007..3768)
                     /locus_tag="VHS97_18005"
     CDS             complement(3007..3768)
                     /locus_tag="VHS97_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450154.1"
                     /translation="MFSQQVITGCFAAALCLCIASGNNARAQIGLPGLPDNPTLEYFG
                     LGEQSTPLSGSGDFTNASGYFGNPFSTAGYGSVGGYGISPYGYGVETGQVASGYQPAG
                     FLYSNVYQGARPRTTVAYQPLYDAITAVPGWNRSAHRTRRRLHSVSNAERTSNIIPFD
                     GTGKIAWPSTISSDPTTTKLRQAAEESVRTVVSESKSTVHASVRPVVEAKNKLSAFEH
                     QVLPEIKNRNRTDGAAVESFFLDLDKALDALTYRY"
     gene            complement(3774..4862)
                     /locus_tag="VHS97_18010"
     CDS             complement(3774..4862)
                     /locus_tag="VHS97_18010"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3450155.1"
                     /translation="MRSIRMTVVLVPLLLGLTAAGPAVAQEAAARPPNGRFAALEAIN
                     ASYQQQRHDLECRRITDLAALAEKSSGPEADAALRQMFSLAIAHSLCPQAQDAAQRCL
                     RAAKSSPEVRALAALVQVLARTDKGEHDRAIADLKVIFQQAAAGGREGRPQDTDLALG
                     VGEALLERLIQDGRYDVARKLCDVACDEEAPAAVKDHFEDRMARIDLVGKPAPPIAGT
                     DVDGKPVSLAEFRGKVVLIDFWASWCPPCVAAIPTLKALSQKYQDRGFVILGINVDAM
                     HEDVKETDAALQQVRRFLVKHRVGWINVLSGKGASDFAAAYRVEQIPASFLVGRDGSV
                     VAVDQHGEMLEQAVVRALEDSTKRREKD"
     gene            complement(4916..>5893)
                     /locus_tag="VHS97_18015"
     CDS             complement(4916..>5893)
                     /locus_tag="VHS97_18015"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEX3450156.1"
                     /translation="SAPANDDREALPPFTTPKTDVVRTTQPAAGPEQLSPRLDPSAVS
                     EASPLTGLDVLQLEAATKPALPRAQELSRDADPPAEQVAPSVRKFGSDIPTASATDHG
                     RGPEVQARSAGEKEAWGDAVDPDGDCKFELDPPEDKVRIIVPGKTHILSAEIGRVNAP
                     RILRDIKGDFDVSVRVAGTDHAGGKATTTLYSPYHGAGLLIWQDPKNYVRLEIATDLQ
                     HGKPRPYVNFEYRKEGVLAATSGMKNGDNSNHLRLSRRGDEINASFGPDGVRWTSFPT
                     LTAKLNDRLKVGVAAINSSTKPLTAELEGLEVSRRPEEGTDPKTGGLNP"
BASE COUNT         1038 a         1798 c         1804 g         1253 t
ORIGIN      
        1 tatccaagtg gtcgctctca cccaggggga tgatcgcata ggactcgggg agatccgtca
       61 agattaccac ctgggtcgac gactgcccgg tgtgcccgat catccgcttg cggtcggcgg
      121 tgggagcgaa actgatggcc ctcggagtgg ccatgccgac gtcttgcagc gagccgccgc
      181 cgacgggcca cgagcggtcg gagccgcgcc gtccgacccg gaaatactcg ccgaacggga
      241 cggccactga gccgaaattg gcttttgccc acgccgctcc ctggcgcagg gcgtgtagca
      301 gttgcgcatc ggaaacgtcg cccggaggct cggtcttgtg cgccagatcg ccgctcaact
      361 ctttcttgaa tgcataaaag gcgagcgccc cttttgagtc gggtgcgctc tggcgatccc
      421 actcgtggat cagcgaatac gcttctttta tgtctgccgt cttgtcagcg tcggcagcct
      481 tctgccaggc ctccttgagc ctcgcttgcc aacgttcagc gtgccagact tgcgtgctga
      541 acgcaatgtc gatcgcttgc tcgactgtca ctttgcaggc agcgtcgagc aggtcggtca
      601 ccatctcggc gcgctgatga gcatcgcccg agggagacgc gttgtagatg tgaggatttt
      661 gggcatagtg ctcatggagc ggctggtcac tattcatcat cgccgccggg gagcagttgc
      721 agttctgcat ccagccgcac ggcggattct cgatctgtac taagtcggaa atcgggtgga
      781 ttccctgcca ctcggtcgtc gaggtatcgc cggggatcgg tcggctggga tcgactccct
      841 tgggacgtat cggcactcgg ccgtttcgta ggtaatagat gtccccctgc acggtgccga
      901 ccatgatgtt ttgcgccatc aactggcagt gcgacaacgc ttgcttcatc tcagtgagat
      961 tcttcgccgt catcatttca taacactggt cggtcaggcc gacttcgtcg gcataaggaa
     1021 tggccatggc gtaagccttg ccgcttttgc gagcgacgat cggcccatgg tgggagaacg
     1081 tcagcgtcac gtcgcgcggc tcaactcgat cgccgttctt gacgttgatc gtttccttgc
     1141 gaatcgtgaa atcccgccat tcgccttcgt agcgatactg gtcggggttc gctgggttca
     1201 gcacttcctc aaagatgtcc gacgtatcag ggccaccggt ggtcatcgcg atcgagcagt
     1261 agcggctgtg ccccaacgtc ggcagcggga cgcccaggat cgaaactccc gccacgttaa
     1321 attctggcga gtaaatcctc acctcgtaaa accgcatggc gtcgtaccac gggacgtgcg
     1381 ggtcgatgat tgcgatgggg gcgtttatgg ccgtgcgctt gggcgcgatc aacatttcgt
     1441 tcgagccgcg gtaactgagt ggcccgaact caacgccggc gcgtttcaag tcggcggccg
     1501 cttcccctat cggccagttc cagatgatat accgccccag cgccaccgcc tgccacggct
     1561 gaatcggtcc gacccacgac ggaacctgct ccggatgctc tttcatgtac tgcttgatcc
     1621 cctcctggta ttgctcgatc acgccccgca tcttgggact tatctcgttg tatttggcct
     1681 ggctaacggc ggcgtgccgc atcacacgct ggcgaaggtc ggcgtcgaaa tggtcagcgc
     1741 caaatacctc ggccatggtt ccggcggcgc gtctgtagtt tttcagcagc tcttcgaggc
     1801 ggtcttccgc ctgggcgtag ccagccgcat aggcagcatg ttcgaggctg ggggcgaaga
     1861 tgtggggaac gccaaactca tcgcgtcgga tcatcgtgga cttgtcgtcc gccctgacaa
     1921 ggcctggatt catcgcgacc aggcccgcaa caagatggga aatggcgaaa agcaaaagtc
     1981 gtttcatcgg ttgccgccca tgaaagccgg gccagccggc gataagtcaa gtcgccgctg
     2041 gcccggaatg atcggtttgt cacggacggc attgtaccac gagattggta tccgatctgc
     2101 tagcgcggac cgacaggcgt cgcggtcgtt gatttggccg ccccgccggc ggctcgctcc
     2161 gattgccagg ggacatcggc cgcctgatag acctcaccgg tccgcttgtt ctgcacaaag
     2221 atggccagcc gcagattctg ccgcttccag ccgggctcga tcgaaaaagt aaaccgttgg
     2281 gttgccggcg agctgccgtc gagctcgata aagtcgtatt tcgtcagccg tgctggaaag
     2341 cgcgcgacca gtgctttgcc ggcattttcg cccgagccaa tttcggtgac gacgttgtct
     2401 tcgcgtagaa cggcgcatac cagcagaggc gttttttcgg cctgggcaga ccggctcgtc
     2461 accttgatct cggcagtacc tgcaagcccg ttgctcttca gatccagctt ggtatccagg
     2521 ctcaccgcgg gctttcgtaa aagcgctttg cggatcgccg gttcggccga ggcggcatcc
     2581 cggccgttaa ccgactgctg gccatcgatc atcatcatcg gagtgtagta gagaccgtac
     2641 tccgggttct tgggcttggt gtaaagctcg ttgtagttca tctgacgctg gctatacaaa
     2701 tggtcggaga atacatccct ccacggatta ttgaagtagt cgacatggaa cgcgatcggc
     2761 acgatcttgc cttcgcgttc cgcaagtgcg cccagcagtt tctcggcctc agggcacatg
     2821 tcgcagcctt gcgaggtgta aagctcgacg agcaccaagc gctttgaatc gtcagatttc
     2881 tcggccccgg ccgactgctc gtggccggca ccgaccgcga tcgctataat tacacccgca
     2941 aaagtcgttt gccaattcat gagcatcctc cggtttgaac tcggccgacc acacgggccg
     3001 acagatttag tagcgatatg tcaacgcatc gagggccttg tcgaggtcga ggaaaaagct
     3061 ctcgaccgcg gcgccatcgg ttcggtttct gttctttatt tcgggcagca cttggtgctc
     3121 gaaggccgac agcttgttct tggcctcaac gaccggccgc actgacgcgt gcacggtcga
     3181 cttcgattca ctaacgactg tgcggacaga ctcttcggcg gcctgacgca gcttcgtcgt
     3241 ggtgggatcg ctcgagatcg tgcttggcca tgcgatctta ccggtcccgt cgaaaggaat
     3301 gatgttcgat gtgcgttcag cgttcgagac cgaatggagc cggcgtctcg tacgatgtgc
     3361 gctgcgattc cagccgggaa cggccgtgat ggcgtcgtaa aggggctggt atgcgacggt
     3421 cgtccgcggt cgtgccccct ggtagacatt gctgtaaagg aagcccgccg gctggtagcc
     3481 cgaggcgacc tggccagtct cgactccata accgtagggc gatatgccgt agcctccaac
     3541 cgaaccatag cctgccgtac tgaatggatt gccgaaataa cccgacgcgt tggtgaaatc
     3601 tccactgccg ctgagcggcg tcgactgttc gcctaagccg aaatactcga gcgtcgggtt
     3661 atcgggcaaa cccggtaagc cgatctgcgc gcgagcattg ttaccgctcg caatgcacag
     3721 gcagagagcc gcagcgaaac aaccggtgat aacttgttgt gaaaacatgg tgattagtcc
     3781 ttctcgcgac gtttcgtgct gtcctcgagc gcgcgtacca cggcttgctc gagcatttcg
     3841 ccgtgttgat cgacggcgac aacagagccg tcgcgaccga cgagaaagct ggccgggatc
     3901 tgctcgacgc ggtaggccgc ggcgaaatcg ctcgcccctt tgccgctcag cacattgatc
     3961 catccgaccc ggtgtttgac gagaaagcgg cggacctgct gcagggccgc atccgtctcc
     4021 ttgacgtctt cgtgcatcgc gtcgacattg atacccagaa tgacaaaccc gcggtcctga
     4081 tatttctggg aaagtgcttt gagtgtggga attgcggcga cgcacggcgg gcaccagctc
     4141 gcccagaagt cgataagcac gacctttccc ctgaattcag ccaatgacac gggcttgcca
     4201 tcgacatcgg tgccggcgat cggcggcgcg ggcttgccga ccaagtcgat gcgggccatt
     4261 cgatcctcga agtggtcttt cacggccgcg ggcgcctctt cgtcgcaagc cacatcgcat
     4321 agctttcgcg cgacatcata ccggccgtcc tggatcagac gctccagcag cgcctctccc
     4381 acgccgagcg cgagatcggt gtcttgcggc cgtccctcgc gtccgcctgc ggcggcctgc
     4441 tggaagatga ctttgagatc ggcgatggcc cggtcatgct cccccttgtc ggtccgggcc
     4501 agcacctgaa ccagggccgc cagggcgcgg acctccgggc tgctcttggc cgccctgaga
     4561 cagcgctggg ccgcatcctg ggcttgggga cagagactgt gcgcgatcgc caggctgaac
     4621 atctggcgga gtgcggcatc cgcctcgggg ccggatgact tctcagccag ggccgccaga
     4681 tcggtaatcc gccggcattc cagatcgtgc cgttgttgtt gatacgaggc gttgatcgcc
     4741 tcgagcgccg cgaaacggcc gttcgggggc ctggccgccg cctcctgggc gacggccggg
     4801 cccgccgccg tcaggccgag tagcaaggga actaggacca cggtcatccg gatcgagcgc
     4861 atcacgtttt gtgctccttt cgagctcaag tagtgcgatt atcgttcaaa gtcggtcatg
     4921 ggttaagccc accagtcttt ggatcagttc cttcttccgg tcttcgcgag acctccaagc
     4981 cctccaattc ggcagttagc ggcttggtcg agctgttaat cgccgcgacg ccgaccttca
     5041 gtcggtcgtt gagtttagcg gtcagagtcg ggaacgaggt ccagcggact ccgtcgggcc
     5101 cgaaggaggc attaatctcg tcgcctcgcc gactaagccg gagatggttc gagttgtctc
     5161 cgttcttcat gccgctggta gcggcaagga caccttcctt gcggtattca aagttgacgt
     5221 atgggcgcgg cttgccgtgt tggagatccg tggcgatctc caggcgaacg tagttcttcg
     5281 ggtcctgcca gatgagaagc ccggccccgt gataagggga ataaagggtc gtcgtcgcct
     5341 tccctcctgc gtggtcggtg ccggcgaccc gcacgctcac atcgaaatca cccttgatgt
     5401 cgcgcaatat gcgcggcgcg ttcacgcgac cgatttcggc gctgagaata tgggtcttgc
     5461 ctggcacgat gatccggacc ttgtcttcag gcgggtctag ctcaaactta caatcgccat
     5521 cggggtccac agcatccccc caagcctcct tctctcccgc cgatcgcgcc tgtacctccg
     5581 gccctcgccc atggtcggtg gcgctcgcgg tcggtatatc gctgccgaac tttctcacac
     5641 tcggggcgac ttgctcggcc ggcgggtctg cgtctcggga aagctcctgc gcgcgcggta
     5701 gagcaggttt agtcgcggcc tctaattgca gtacgtcgag accggtgaga ggactcgcct
     5761 ccgatacggc acttggatca agccgcggac tgagctgttc ggggccggca gccggttgag
     5821 tcgtccgcac gacgtcggtc ttcggagtcg tgaatggcgg aagggcctca cggtcgtcat
     5881 tggccggggc tga
//
LOCUS       DATAQL010001044         5150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3922944, whole genome shotgun sequence.
ACCESSION   DATAQL010001044 DATAQL010000000
VERSION     DATAQL010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3922944"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VHS97_18020"
     CDS             <1..623
                     /locus_tag="VHS97_18020"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HEX3450157.1"
                     /translation="AESLRGLSGLDMVVDAGPTHYQNFATVVRVDEDRWSIERQGVVD
                     AATLAETSSVIILFVCTGNTCRSPMAEAICKLLLAKRLGCPLDELDRRGFVVRSAGVA
                     ATNGVPAAAHAIDVIRALGGSLENHRSRRIATSLVRQADCIFAMTADHLDDLLRAMPE
                     VEPRAFLLDPGGGDVADPVGCDHETYRRTAHMIESMLTQRLDELGV"
     gene            854..1711
                     /locus_tag="VHS97_18025"
     CDS             854..1711
                     /locus_tag="VHS97_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3450158.1"
                     /translation="MAVATVTDGVLAPDSGTTPVHRLGEHVFDAIAEIGDVAHFSMLT
                     LLWIVRRRSRWSVLVPIFYAIGVKSVSVVAITGTFIGMVLAVQAYHQFSMMGLATRLG
                     SVINISIVRELGPVLAATMLAGRVGSAMAAELGTMRVTEQIDALSALGTNPIYYLVVP
                     RFLACVLLIPLLTLMGDFMGVIGGAVVSTQILNIDSFAYWMHTRDFVDSLDIFAGVFK
                     SYFFGAAIALVSCHRGFNSKAGAEGVGLAATEAFVLSFIAILVLDFVLGLAWNNIYHS
                     LWPQAQGLL"
     gene            1708..2574
                     /locus_tag="VHS97_18030"
     CDS             1708..2574
                     /locus_tag="VHS97_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3450159.1"
                     /translation="MSQGQAQSGARRDDLIELRDVNMRFRYQTVLRDISLTIHRGETL
                     CVIGESGCGKTVMLKLIIGLLKPTQGSVWFDGRNLAQLDGKSLVKMRLRFGFLFQMAA
                     LFDSLSVFDNVAFGLREHHMCDETEVRQIVADRLQEVGLPPEVETKKPAELSGGQRKR
                     VGLARALAIGPEVMLYDEPTTGLDPIMSDVINELILQTQQGKKTTGVVVTHDMKTVFK
                     VADRVVMLYPLSRLAPGESQILYDGPPAELEQSPDPRVGQFVRGEAGERLREMAQQRT
                     LASSGRPALNSR"
     gene            2604..3704
                     /locus_tag="VHS97_18035"
     CDS             2604..3704
                     /locus_tag="VHS97_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX3450160.1"
                     /translation="MNERLMQFRIGMFVIVAGLVLTMMIVWFGESPAILRDQVYLKVH
                     YAEAPGVIEGVPVRKSGIRIGEVFSNEFDERPGQPDGVIVTLALERRYKLHKGTVPRL
                     NRSLIGDVTVDMMPGTAPGNLETGKMPALAPVIEGEVAPDPSKALAAATKAFESAGDT
                     LKAINSAATGLARISDSAQNLDGFLTSVADAGRNVSKAAQGLDRVIKNNEADIQPMVA
                     DLRQVARKLDESIGQGALKSGIDKFASAAARLDSGIAQIDPLLKDLGSPVDRRPTSDL
                     GQAIRRINLLAADLELLTSKLRDGRGGLNNDGTLQKLITQAELHDNFNTMALTATRAL
                     VELKIVLAAIRNFVEKVSRDPSALTRGALQSR"
     gene            3837..>5150
                     /locus_tag="VHS97_18040"
     CDS             3837..>5150
                     /locus_tag="VHS97_18040"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450161.1"
                     /translation="MRTIPRREFLGDTAALAASMAVIPGFASADTPKTGEADTKPASA
                     NETLRVAVCGVKGRGMEHIAGWNKLKDVRITALCDVDLNVTGPATKAVAKRNGTEPPV
                     VQDIRRLLDDKSIDAISVATPNHWHALATIWACQAGKDVYVEKPVSHNVSEGRRMVEA
                     ARKYERIVQTGTQCRSHQGIQDAMAFLRSGKLGQIYMAKGLCYKPRGSIGHKADSAVP
                     TGVDYDIWTGPAPMRPFNANRFHYNWHWFWDTGNGDLGNQGIHQMDLARWGLGKNELP
                     KAVMASGGRFGYTDDGQTPNTLQVSFEFDDCELQFEVRGLITNNESQVKIGDIFYGTE
                     GILAITSYESWQVFFGPKLEPGPSGRGSGDHYANFVKAVKARDPKLLNADIEQGHLSS
                     AYCHLGNIAYRLGRKLHVNPSAESFINDSEADLMLTRDYRAPFVVP"
BASE COUNT         1011 a         1617 c         1546 g          976 t
ORIGIN      
        1 cggccgagtc actccggggt ctgtccgggc tggacatggt cgtcgacgcc ggacccacgc
       61 attatcaaaa cttcgcgacc gtagttcgcg tcgacgaaga tcgctggagt atcgagcggc
      121 agggagttgt cgacgcggcc actctcgcgg aaacttcgag cgtgatcatc ctattcgtgt
      181 gtacggggaa cacctgccgc agccccatgg cggaagccat ctgcaaactg ctgctggcca
      241 aacgccttgg ttgcccgctc gacgagctcg atcgccgtgg attcgtggtg cggtccgccg
      301 gcgtggcagc caccaatggc gtacctgccg ccgcccatgc catcgacgtg atccgcgctc
      361 tcggaggttc gctcgaaaac catcgcagcc gccggatcgc gacgagcctc gtccgccagg
      421 ccgattgtat cttcgccatg acggccgacc atctcgacga cctgctccgc gccatgcccg
      481 aagtcgagcc ccgcgcattc ctgctggacc ccggcggcgg cgatgtggct gaccctgtcg
      541 gctgcgacca cgagacctat cgtcgcactg cacacatgat tgagagcatg ctgacccaac
      601 gcctcgacga gttgggcgtt tgacgacacc aagaaatagt cgtgtgcaca ctcacgacgt
      661 ccccacctcg tcgactgaat gcatgtctgt ctgctcgaaa tgtcgtcggc cttgtcaatt
      721 tccgcgtaat gtcgagaata actttgattg cacgtggcga ggaacgacat cgtcggcata
      781 tgccaggcgc aggagttgcc tggagaacgg ccgcaaggcc gtttcatgac accaaggtag
      841 gagcgagctg gcgatggcgg ttgccacagt caccgatgga gtgctcgcgc cggattcggg
      901 gacgacgccc gttcaccgcc tgggagagca cgttttcgac gcgatcgccg aaatcggcga
      961 cgtcgcccat ttctcgatgc tcaccttgct ctggatcgtg cgccggcggt cgcggtggtc
     1021 ggtcttggtc ccgatcttct atgcgatcgg cgtcaagagc gtgtcggtag ttgcaattac
     1081 gggaaccttc atcggcatgg tcctggcggt gcaggcgtat catcaattct cgatgatggg
     1141 tttggcgacc cgtctgggat cggtgatcaa tatttcgatc gtccgcgagc tagggccggt
     1201 gctggcggcg acgatgctcg cgggccgggt cggctcggcg atggccgcgg agctgggaac
     1261 gatgcgcgtc accgagcaaa tcgatgccct gtcggcgctc gggaccaacc ccatttacta
     1321 cctcgtggta ccgcggtttc tggcctgcgt gctcttgatc ccgctcttaa ctctgatggg
     1381 ggatttcatg ggcgtgatcg gcggcgccgt cgtcagcacc cagatcctca acatcgactc
     1441 gttcgcctac tggatgcaca cccgcgactt cgtggacagc ctcgatatct ttgccggagt
     1501 gttcaagagc tatttcttcg gcgccgcgat cgcgcttgtc agttgccacc gcggcttcaa
     1561 ttccaaggcg ggcgccgagg gggtgggatt ggccgctacc gaggccttcg tcttgtcgtt
     1621 catcgccatc ctggtgctcg attttgtgct gggactcgcc tggaacaaca tttaccactc
     1681 gctttggccc caggcccagg gactcttatg agccaaggtc aggcacagag cggtgccaga
     1741 agagacgacc tgatcgagct ccgcgacgtc aacatgcgct tccgctacca gacagttctc
     1801 cgcgacatta gcctgacgat ccatcgcggt gagaccttgt gtgtgatcgg cgagagtggt
     1861 tgcggtaaga cggtgatgct caagttgatc atcggtctct taaagcccac gcagggaagt
     1921 gtttggttcg acggccgcaa tctggcccag ctcgacggca agtcgctcgt caagatgcgg
     1981 ctgcggtttg gatttctgtt tcagatggcc gcgttgtttg acagcctctc ggtctttgac
     2041 aacgtggcct tcggtctccg tgagcatcac atgtgcgacg agacggaggt gcgccagatc
     2101 gtcgccgatc ggcttcagga agtcggcttg cccccggaag tcgagacgaa aaagcccgca
     2161 gagctctcgg gaggtcagcg aaaaagggtc ggactggcac gtgcgctcgc gatcggcccg
     2221 gaggtgatgc tttacgacga gcccaccacc gggctggacc caatcatgag cgatgtgatc
     2281 aacgagctta tcctccagac ccaacaaggc aaaaagacga ccggagtggt cgtcacccac
     2341 gacatgaaga ccgttttcaa agttgccgac cgggtggtga tgctctatcc cctgtctcga
     2401 ctcgcgccgg gcgagtcgca gatcctttat gacgggccgc cggccgaact cgaacaatct
     2461 cccgatccgc gggtcggcca gttcgtcaga ggagaggcgg gcgaacggct gcgcgagatg
     2521 gcccagcagc gcaccctcgc gtcatcaggg cggccggcac tcaattcccg ttaatggcga
     2581 tgccgttcga ttaggtggtg aagatgaacg agcgcctcat gcagttccgg atcggcatgt
     2641 tcgtcatcgt ggcgggcctg gtcctgacca tgatgatcgt ctggttcggc gagtcgccag
     2701 cgattttgcg cgaccaggtc tacttgaaag tccattacgc cgaggcacca ggggtcattg
     2761 agggtgtccc cgtacgcaag agcggaatcc gcatcggcga ggtcttttcc aatgaattcg
     2821 acgaacgacc cggacagccg gacggcgtca tcgtaacttt ggccctggaa cgccggtaca
     2881 aactgcacaa ggggaccgta ccgcgactca accgatcgct gatcggcgac gtcaccgttg
     2941 acatgatgcc gggcactgcc cccgggaatc tcgaaaccgg caagatgccc gccctggcgc
     3001 cggtgatcga aggggaagtc gctcccgatc cctcgaaggc ccttgccgcg gccaccaagg
     3061 cctttgaaag tgccggcgac actttgaagg caatcaacag cgcagccacc ggcctcgctc
     3121 gcatctccga cagtgcccag aatctcgacg gtttcctcac gtccgtggcc gacgccggcc
     3181 gcaacgtctc caaagccgct cagggactcg atcgcgtgat caaaaacaat gaagccgaca
     3241 tccagccgat ggtcgccgat ctgcgccagg tcgcgcgcaa gctcgacgaa tcgatcggtc
     3301 agggtgcgct caagtcgggg atcgataagt ttgcgtccgc cgcagcccgg ctcgactcgg
     3361 gtatcgcgca gatcgacccc ctcctcaagg atctggggtc gccggtcgat cgcaggccga
     3421 ccagcgacct gggccaggcc atccgccgca tcaaccttct ggccgccgat ctcgagctct
     3481 tgaccagcaa gctgcgcgac ggccgcggcg gactgaataa cgacggaacc ttgcaaaagc
     3541 tgattaccca ggccgaactg cacgacaact tcaatacaat ggcgctcacg gccacccggg
     3601 ccctggtgga gctcaagata gtcctcgccg cgattcgcaa ctttgtcgaa aaggtctcgc
     3661 gcgatccctc ggcgctcacg cggggcgccc tgcaatccag gtagcgcacg ttggcccata
     3721 gacattctca caatcgctat gggcttgact tgagccaaat tctgccgttt agttgagtga
     3781 tcggcccact cttttctgaa acgtccctcc atccatcctg ttcgagggtt tcaacgatgc
     3841 gcacgattcc acgtcgtgag ttcctaggag acacagccgc actggccgca agcatggcgg
     3901 tgattccggg atttgcgagc gccgacacac ccaagacagg cgaggccgac accaagccgg
     3961 ccagcgccaa cgaaacgctc cgcgtcgcag tgtgcggcgt caaggggcgc ggcatggagc
     4021 acatcgccgg ctggaacaaa ctcaaagacg ttcgcatcac tgcactctgc gacgtcgact
     4081 tgaatgtcac tggaccagcc acgaaagccg tcgcgaagcg caatgggacc gagccccccg
     4141 tcgtgcaaga catccgacgg ctcttggacg acaagtcgat cgatgccatc tcggttgcca
     4201 cgcccaatca ctggcatgcg ttggcgacga tctgggcgtg ccaggccggc aaggacgttt
     4261 acgttgagaa acccgtcagc cacaacgtct ccgaggggcg gcggatggtc gaggccgcac
     4321 gcaaatacga acgaatcgtc cagaccggaa cccagtgtcg cagccaccag ggaatccagg
     4381 acgccatggc atttctccgt tccggcaagc tgggccagat ctatatggcc aaggggcttt
     4441 gctacaagcc acgcggttcg atcggccaca aggccgacag tgcagtccca actggcgtcg
     4501 attacgatat ctggaccgga ccagcgccca tgcgcccgtt caacgccaac cgcttccact
     4561 acaactggca ctggttctgg gacaccggca acggcgacct cggcaatcag ggcatccatc
     4621 agatggacct ggcccgctgg ggcctgggca agaacgagct gcccaaggct gtaatggcct
     4681 ccggcggccg atttggctat accgacgacg ggcagactcc caacactttg caggttagct
     4741 tcgagttcga cgattgcgag ctgcagttcg aggtgcgtgg gctcatcacc aacaacgagt
     4801 cccaggtcaa aatcggcgac atcttctacg gcaccgaggg gatcttggcg atcaccagct
     4861 acgagtcatg gcaggtcttt tttgggccga agctcgagcc agggcccagc ggcagaggat
     4921 cgggagacca ctatgccaat ttcgtcaagg ccgtcaaggc gcgcgatccc aaactcttga
     4981 acgccgatat cgagcaaggc cacctctcaa gcgcatattg ccacctgggc aatatcgcct
     5041 accggctggg gcgcaagctg cacgtgaacc cctccgccga gtcgttcatc aacgactccg
     5101 aggccgatct gatgctcacc cgcgattatc gagcgccgtt tgtcgtgccg
//
LOCUS       DATAQL010001045         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3922981, whole genome shotgun sequence.
ACCESSION   DATAQL010001045 DATAQL010000000
VERSION     DATAQL010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3922981"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="VHS97_18045"
     CDS             complement(<1..2017)
                     /locus_tag="VHS97_18045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3450162.1"
                     /translation="MVDREPDDDGAPHVELDQVLADYMERLDRGEFVDRERFVAEHPD
                     FAGDLYIYFEGSDDLMRLTLPAYVAEQEHTMSLTREEAPVAAAGSKTRDHVPYFGDYE
                     LLEEIARWGMGVVYKARQVSLNRPVALKMILDGRLASSADVARFRIEAEAAANLDHPH
                     IVPIYEIGEHLGQHYFSMKLIRGGSLDDPSVRPGSDLRAVARLVATIARTVHHAHMRG
                     ILHRDLKPANILIDAEGGPHLTDFGLARRVGGEASHSSGGGIAGTPSYMAPEQAIGRC
                     RELTTAVDVYGIGVILYELLVGHPPFRGDSPVEIIWRVVEQPPPSLRAQNRSIPRDLE
                     TICLKCLEKEPGRRFRSAEALAEDLERWLDGRTILAQRTTLPERSWRWCRRNPVLATL
                     IATVASLLVAIALSATSSAARLRESLTKVEIAEREGREKLWESYLAQAQANRYSGHLG
                     RQFGSLEVLAKAAAIRPDPRLRDEALACLPLLDLRLIRRWEAFPAGSQVWPAFDGECQ
                     RYVRFSAEGTASVRRVDDDQEIVRIPCRKGWNHWPQFERQGRFLHWIDQLPSHEWQFE
                     LWDVSRGKRIALDPPSPVGPRSDFRRDGRELAKTSADSVEFYDLPLGKRIGGWSVGGR
                     AIHVRYHPDGRRLAVQVENEAAIRICDRDTGKVIQRFELPGSAGDVDW"
     gene            complement(2090..2701)
                     /locus_tag="VHS97_18050"
     CDS             complement(2090..2701)
                     /locus_tag="VHS97_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450163.1"
                     /translation="MQESIAEFVDLIGRARRGDDAALGRLMEENRAALLAEAERQLRG
                     RLAARVDSSDIIQQTFLEAHRDFERFEGQTEIQFSSWLRTILDHNIAGELRDHTRSAK
                     RDVRRERSLDEGRGGGEPLWRKIVAGHSTPSQRAMRAEDFARLAMALRTLTDDQREAV
                     RLRHLERWPLARIAERLGRSPAATAGLIKRGVQALRERIIEDE"
BASE COUNT          487 a         1000 c          987 g          538 t
ORIGIN      
        1 gccagtcgac gtcaccagcg ctgcctggga gctcaaagcg ttggattacc ttgcccgtat
       61 cccggtcaca gattcgaatc gctgcttcgt tttcgacctg gacagcgagt cttcttccat
      121 ctgggtgata acggacgtgt atcgcgcggc ctcccacgct ccaaccgccg atacgcttgc
      181 caagcggaag gtcgtagaac tcgacggagt cggcgctcgt tttcgccagt tcccgaccgt
      241 cgcgacggaa atcagacctg ggtccgacgg gactcggtgg gtcgagggca atccgttttc
      301 cccgtgagac gtcccagagt tcaaactgcc actcgtggga aggcaactgg tcgatccagt
      361 ggaggaaacg gccttgacgc tcgaactgcg gccagtgatt ccagcctttt ctgcatggaa
      421 ttcgaacgat ttcctggtcg tcgtcaactc tgcggacgct cgctgttccc tcggcgctga
      481 agcgcacgta gcgctggcac tcgccgtcga aggccggcca gacttggctc cccgccggaa
      541 aggcttccca gcgtctgatc agacgtaggt cgagtagcgg gaggcaggcc aaggcctcgt
      601 cacgcagcct gggatcgggg cggatggcgg cggccttcgc cagcacttcc aggctgccga
      661 actgccgtcc gaggtggccg ctgtaccggt tggcctgggc ctgggccaag taggattccc
      721 agagcttctc gcgcccctcg cgctcggcga tctcgacctt ggtcaggctt tcccggaggc
      781 gggcggcgga actggtcgca gagagtgcaa tcgctacgag cagtgacgcc acggtcgcga
      841 tgagggtcgc caggaccggg ttgcggcggc accaccgcca cgaccgctct ggcagggtgg
      901 tccgctgcgc caggatggtc cggccgtcca gccatcgctc caggtcttcg gccagcgcct
      961 cggccgatcg aaagcgccgc cccggctcct tctccaggca tttcaggcag atcgtctcga
     1021 gatcgcgggg gatcgagcgg ttttgcgccc ggaggctcgg cggaggctgt tcgacgacgc
     1081 gccagataat ctccacgggg gagtcgcccc gaaatggggg atggcccacc agcaactcgt
     1141 acaggatcac accgatgccg tagacgtcga ccgccgtggt cagctcgcgg caccggccga
     1201 tcgcctgctc gggcgccatg taggagggcg tgcccgcgat ccccccgccc gaggaatggc
     1261 tcgcttctcc gccgacccgc ctggccaagc cgaagtcggt caggtgaggc cccccctccg
     1321 cgtcgatcag gatatttgcc ggtttgagat cgcggtgcag aatgccccgc atgtgggcgt
     1381 ggtggaccgt ccgcgcgatc gtcgccacca accgggcgac ggcacggagg tcggacccgg
     1441 gccggaccga aggatcgtcc aagctcccgc cccgaatcag cttcatgctg aaataatgct
     1501 gacccaggtg ctcaccgatt tcgtagatcg ggacgatgtg ggggtggtcg agattcgcag
     1561 ccgcctccgc ctcgatccgg aatcgtgcga cgtcggccga cgaggccagt cgtccgtcca
     1621 ggatcatctt gagggctacg gggcggttga ggctgacctg gcgagccttg tacacgacgc
     1681 ccatacccca ccgcgcgatc tcttccagta actcgtaatc gccgaagtag gggacgtggt
     1741 ctcgcgtctt ggagccggcg gccgcgaccg gcgcctcctc ccgcgtcagg ctcatggtgt
     1801 gctcttgctc cgcgacgtag gccggtaggg tcaggcgcat gaggtcgtcg ctgccttcga
     1861 agtaaatgta caagtccccg gcgaaatcgg ggtgttcggc gacgaaccgc tcgcggtcca
     1921 cgaactcgcc ccggtcgagg cgctccatgt aatccgccaa gacttggtcg agctcgacat
     1981 gcggtgcgcc gtcgtcatcg ggctcgcggt cgaccatgga taaacctctc gcacgcttgc
     2041 ttcgcggccg ggcccgcggc ggccgccgcc gtgcgaccac cggactctct tactcgtcct
     2101 cgatgattcg ctccctcaac gcctggacgc cccgcttgat cagcccggcg gtggccgccg
     2161 ggctccggcc caaccgctcc gcgatccggg ccaacggcca cctctcgagg tgacgcaggc
     2221 ggacggcctc tcgctggtcg tcggtcagtg tcctgagggc catggccagg cgtgcgaagt
     2281 cctccgcccg catggcgcgc tggctgggcg tcgagtgccc ggcaacgatc tttctccaga
     2341 gcggttcccc accgccgcgg ccttcgtcca gcgaccgctc tcgacgcacg tcacgcttgg
     2401 cggaccgggt gtgatctcgg agttccccgg cgatgttgtg atccaaaatc gtccggagcc
     2461 aggaggagaa ctggatctct gtctggccct caaaccgctc gaagtcgcgg tgcgcctcca
     2521 ggaacgtctg ctggatgatg tccgaggagt cgacccgggc ggcgaggcgt ccgcgaagct
     2581 gccgctccgc ctcggcgagc agagccgccc gattctcctc catgagtcgt cccaaggccg
     2641 cgtcatctcc ccggcgtgct ctcccgatca agtcgacgaa ctccgcgatg gattcttgca
     2701 tggcacggtc ctcgtataga ggcacggaaa tcctcgaggc tgggcgcgcc gcgtctcgaa
     2761 aaatggaatt agcagactga tcgtcgctcc ggagcattat gacgggggcg gctcagtgtg
     2821 actgtcggtc gattgtgggg gcgggacgtg cagagcaagc cggtcctcag ccgggacgaa
     2881 cagtatcatg catgatggat cgctagcccc gaaaggccgc gatgggcgaa gttgaagtca
     2941 ccaggtggcg ctccgttgac gcgcatggag acttgagaat ggttcaccga cctcccgggt
     3001 ccgggccccg cg
//
LOCUS       DATAQL010001046         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3006588, whole genome shotgun sequence.
ACCESSION   DATAQL010001046 DATAQL010000000
VERSION     DATAQL010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3006588"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1779)
                     /locus_tag="VHS97_18055"
     CDS             complement(40..1779)
                     /locus_tag="VHS97_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450164.1"
                     /translation="MSQRKPKIQMRSLRPMAESLETRQLLSSVNVSLTTPTAVAKGTD
                     PDGAQWTLQLFGPGALSVVGVNGTVFNKSTQTSTDSIETITVGGAITTQTRLVGTVHG
                     TNGQSNVFFENLNVTPTGELGKIDLGQVNNFRSVQNGIFAIDMPNFYLAHTDTATPST
                     PSAIHPGAMSAGQIFIPQGVITLRFGGVNVDYTPPGGTPLTQTNQNNEFQIDLGLPIV
                     QGTSIIVNSVNSDAHANTSSATQPFQDYATFLVGGRLNLFQANQITGNTTAGLVPSQF
                     PNQTPATTSDPGGTYVFADGTPGGVTGQIGNLRIGGAATNFTTLISENPENVVAAEGQ
                     LDAKISNFSIGGQTKNVLLVAPSGSRNVVFGLGMDNVTINSDTISSLTANRDVSNSQV
                     TVSRSIGNLLVGGDVTNTNINVGESQSLFTFADAPPSNGVFFGGLPPTVAKPQTNPIT
                     GQMEPFAQNGGTIRARIAGSVNGSVISASVDPNPSTLLTQVFGPGQGNLQLPRGVINA
                     KVEGTISNANNPLVSDAGKAFFARVVHLTKGPVIPPSVPYAPYPAPTVYHKGQNALRG
                     LFKIDHHPRIIKK"
     gene            2244..2666
                     /locus_tag="VHS97_18060"
     CDS             2244..2666
                     /locus_tag="VHS97_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450165.1"
                     /translation="MTNPANPRRRPSVHITLQRAARLHRLVRFLATSPRTRASILSDL
                     RIGLRTFYRELELLKRCGVKIRHKNKLYDLVPTAAEALGRLPFPDPQLSFAEMAELAQ
                     CPCPAGQRLAELLATVINQPEPPKKLSRKKPGRKKAGL"
     gene            complement(2806..>3140)
                     /locus_tag="VHS97_18065"
     CDS             complement(2806..>3140)
                     /locus_tag="VHS97_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450166.1"
                     /translation="DRQSEETGHQHDIGEKGQKNDRAPKPADARQLGEESQKTDKKKV
                     DVVAGVITGADRPPRIGGTANLCLSVHAVWLFIPGSVYIVSSSIKNVGHFSTGHHPPR
                     LAWIQPLR"
BASE COUNT          596 a          885 c          956 g          703 t
ORIGIN      
        1 tgaccacgat tctctgcctg gcggcccgtc cggacgccgc tatttcttga tgatcctggg
       61 gtggtggtcg atcttgaaca gccccctcaa ggcgttttgc cccttgtgat agaccgtcgg
      121 agccgggtac ggagcgtagg gcacgctggg cggaatcacc gggcccttcg tgaggtggac
      181 gacgcgggcg aagaaggcct tgccggcatc gctcacgagc gggttattgg cgttgctgat
      241 cgtcccttcg accttggcgt taatgacgcc tcgcgggagt tggagattgc cctggcccgg
      301 tccgaagacc tgcgttagca aggtcgacgg attgggatcg accgatgccg agatcaccga
      361 tccattgacg ctgcccgcga tccgcgccct gatggttcca ccgttctgag caaacggttc
      421 catctgaccg gtgatggggt tagtctgcgg tttcgcgacg gtcggcggca gtccgccaaa
      481 gaagacacca ttgctcggcg gggcgtcggc aaatgtgaag aggctctgcg actcgccgac
      541 gtttatgttc gtgttcgtga cgtcgccgcc gacgagcaag ttgccgatcg agcggctcac
      601 ggttacctgt gaattggaca catcgcggtt ggccgttagt gacgagatgg tgtcgctgtt
      661 gatcgtgacg ttgtccatgc ccaagccgaa taccacgttg cgcgagcccg acggagctac
      721 caggagcacg tttttggtct ggccgccaat ggaaaagttg ctgatcttgg catcaagctg
      781 gccttccgcg gcaaccacgt tttccgggtt ttcgctgatg agcgttgtga agttggtcgc
      841 ggcgccgcca atgcgcaaat tgccgatctg tcctgtgaca ccgcctggcg ttccgtccgc
      901 aaaaacgtat gtgccaccag gatcagatgt tgtagcaggc gtttggttgg ggaactgcga
      961 gggaaccaat cccgcagtcg tattcccggt gatctggttg gcctggaaca ggttcaagcg
     1021 gccgccgacc aggaaagtcg cgtaatcctg gaaaggttgg gtggcgctgg aagtattggc
     1081 atgggcgtca ctgttgaccg aattgacgat gatgctggtt ccttgaacga taggcaggcc
     1141 caagtcgatc tggaactcgt tgttttgatt ggtctgagtg agtggagtac cgcccggcgg
     1201 cgtgtagtcg acattgacgc cgccaaagcg aagggtgatc actccttggg gaatgaagat
     1261 ctgccccgcg gacatcgccc cagggtgaat cgccgagggc gtagacgggg tcgccgtgtc
     1321 ggtgtgagcc aggtagaagt tgggcatgtc gatggcgaag atgccgtttt gaacggaccg
     1381 gaagttattc acctggccca agtcgatctt gcccagctcg cccgttggcg tcacattcag
     1441 gttttcgaag aagacgttcg actggccatt ggtgccgtga accgtgccga ccagccgggt
     1501 ctgagtcgtt atcgctccgc cgaccgtgat cgtctcgatc gagtctgtcg aagtctgagt
     1561 cgacttgttg aaaacggtgc cgttcacccc cacgacgctg agcgcgcctg gaccgaaaag
     1621 ctgcaaagtc cattgcgcgc catccgggtc ggtccctttg gccaccgcag tcggtgtcgt
     1681 aaggctaaca ttaaccgacg agagaagttg acgcgtctca aggctctcgg ccataggccg
     1741 aagggaccgc atctgaatct ttggcttccg ctgcgacatg gatcagatac ccctttccca
     1801 ggaggctagg ccaccgtcat tgtcggtgca cagctcgaat gtcaatcctg acttgagctt
     1861 gcgtcgcctc tccgtccatg gatcacccga gacgggatca tttccgtggc aatacaactt
     1921 gcatgcatcc attacattat gcgatcattc agcaatgcga aactgtggcg gagactccgc
     1981 ctgctttggt caatgatcga caggctgtac ggaattcttg aaagaatcgt tagaattgtt
     2041 tcaatcctct taatccgaag ggtcggcaca tcgtctcttc tttgctggga gaggtactgg
     2101 agtaccttcg gccgaggcgc gcgtttcgca cggctcttaa cgatacttac atgggatgga
     2161 tcctcgatta ctggtcttcg cccgtccgac tcccgcctga tgcgagtcat gtgagggaaa
     2221 ggatagtgag ggatcgtccc gccatgacta accccgcgaa cccgcgacgt cgtccttcgg
     2281 ttcacatcac cttgcaacgt gcggcccggc tgcaccgtct ggttcgcttc ctggcgacga
     2341 gcccacgaac ccgcgcgtcg atcttgtccg atctgcgaat cggcttgcgg acgttctacc
     2401 gtgagctcga gcttctcaag cggtgtggcg ttaaaattcg ccacaagaac aagttgtatg
     2461 acctggtgcc gacggccgcc gaagcgctcg gccgcctgcc tttccccgat ccgcagttga
     2521 gcttcgccga gatggccgaa ctggcccaat gcccttgccc ggccggacaa cggttagcag
     2581 agctgctggc cacggtgatc aatcagcccg agcccccgaa gaaactatct agaaagaagc
     2641 ccgggcggaa gaaagccgga ctgtaaatcg gctcgcagcc gcagccgcag acgcgtggcg
     2701 tctcgttggt gagacgaaac gatgggccct cgccgatcaa gcactcctgg ttacgttccc
     2761 gtcgatcggt ctgggacgtg gagtctgtgg cgcagtgcgg catgtttatc tcaacggctg
     2821 aatccatgcg agtcggggcg gatgatggcc ggttgaaaaa tgtcctacgt tctttatgga
     2881 cgacgacacg atgtagaccg aaccgggaat gaagagccac acagcatgca cactcagaca
     2941 gagattcgcc gttccgccga tccgcggcgg acgatcagcg ccggtgatca caccggcaac
     3001 cacgtcgacc tttttcttat cagttttttg gctctcttcg ccgagctggc gtgcatccgc
     3061 tggtttggga gcacggtcgt ttttttgacc tttttcacca atatcgtgct gatggcctgt
     3121 ttcctcggat tgtcggtcgg
//
LOCUS       DATAQL010001047         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_257218, whole genome shotgun sequence.
ACCESSION   DATAQL010001047 DATAQL010000000
VERSION     DATAQL010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257218"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VHS97_18070"
     CDS             <1..213
                     /locus_tag="VHS97_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450167.1"
                     /translation="RDPTLGGTALLSEAVNRHVASGDLLRCHARAGREVGSRWPIIDA
                     RNALRPDDLRQVAERPEPTPAEGRHY"
     gene            896..>1794
                     /locus_tag="VHS97_18075"
     CDS             896..>1794
                     /locus_tag="VHS97_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450168.1"
                     /translation="MSGDTEKTRAAENLLRIAAGARPIRRPDGRYSASIAIDGHQECH
                     DLESPDVARWLTRRYYESTGRLPSSASLSSTIRALAAHADIARTAEADLVRVGCNASG
                     SSIFLDLGDSAWQGVEIRATGWQVIERPDAHFRRSAGQRALPVPSRDGSIALLRKYIN
                     VDSADLPLLVGCLTAALRPAGPHPIIFLPLRSSVPSRRRCEEDLWAEFMPDYPRIFGG
                     LLDAVAAGMRLRPKVKLAELERMADFTRWGEAAAQGAGWAPGTFVTAYRSNRQAASSV
                     TLEDSPMAQAMLSPAKFHGPWKGT"
BASE COUNT          314 a          592 c          564 g          324 t
ORIGIN      
        1 cgcgatccca ctctcggcgg gaccgctctg ctgtcggagg ccgtcaatcg acatgtggcc
       61 tcaggtgatc tcctgcgttg ccacgctcgc gccggacgtg aagtcggttc gcgatggcca
      121 atcatcgacg cgcgcaacgc cctaaggccc gatgatctca gacaagtcgc cgaaagacca
      181 gagccgactc ccgcggaagg ccgtcattac tgacttcaaa gccccgatgg cccattttgg
      241 cacggattct tgccataccc cgagcaagcg ccacggcgcg ggcggttctg cgtggcaacc
      301 aagtcgtgcg cacgtcggtc cgacaccgaa gcagtttctg cgaagcatca ggcgtgccct
      361 catttggcag atcatgcaca tcccgacagc accgtggaga ttggggcgac tgaaatgggt
      421 gccgcgattc gctgtccgtt agtcggagac tctacccgag gaacggagac ggctgaaccg
      481 ctcacctcca ccccggcgtc gtgggctgca cgtctcggtg cattgagtga gcgctcggtt
      541 ccctgacact ctatgaggaa cggggaacga cttggcgagg ccgctctgtt tccaggccgc
      601 cggtcactgc gcagggtcga acgcctcggt ctgagccgtg gaggtgagcg actctggacc
      661 gtcatcgtgg cacgggcttc cagcccgtgt ccgggaagac cacggacaag gtacccgtgc
      721 cactctaacc cgtgtctggg aagaccacgg gcaagatgcc cgtgccactt gttcaactga
      781 ttcttccgtg gatcctcgcg cctccgatca aggatcgaaa tcgccggtag gcgccgtggc
      841 cgaagcaaca gaagacccat tgagcgggga agaatcgggc ggatccccgc ccaccatgag
      901 tggcgacacc gaaaaaacac gcgcagctga gaacttgctg cggatcgcgg cgggtgcccg
      961 gccaatccgc cggccggatg ggcggtactc ggcgtcgatc gccatcgatg ggcatcaaga
     1021 gtgccacgat cttgaatcgc ccgatgtcgc gcgctggctg acgcgccgct attacgagtc
     1081 gaccgggagg ttgccgtcgt ccgcgtcgct gtcgtcgacc atccgcgcac tggcggcgca
     1141 cgccgacatt gcgcgtacgg ccgaagccga tcttgtccgc gtgggttgta acgcgtcagg
     1201 ctcgtccatc tttctcgacc tgggcgattc cgcctggcag ggagtcgaga tccgggccac
     1261 cgggtggcaa gtcatcgaac ggcctgatgc ccatttcagg cggtcggccg gccagcgggc
     1321 tcttccggtt ccgagccgcg acggctcgat cgccctcttg cgaaaataca tcaatgtcga
     1381 ttccgccgat ttgccacttt tggtcggctg cctcacggcc gcactccggc ccgcgggccc
     1441 gcatccgatc atcttcctcc cgcttcgttc gagcgtccca tcgcgccgcc ggtgcgaaga
     1501 ggatctctgg gccgagttca tgcccgacta cccgcggatt ttcggtggtc tcttggacgc
     1561 cgttgcggcc ggcatgcggc tccggcccaa ggtcaaactt gctgagcttg agcggatggc
     1621 cgactttacc cgctggggcg aagcggccgc tcaaggggcc ggctgggccc caggcacgtt
     1681 cgtgacggcc tatcgatcca atcgtcaggc agcaagctcc gtaacgctgg aagactcgcc
     1741 catggcgcag gccatgctga gccccgccaa gtttcacgga ccctggaaag gaac
//
LOCUS       DATAQL010001048         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4684336, whole genome shotgun sequence.
ACCESSION   DATAQL010001048 DATAQL010000000
VERSION     DATAQL010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4684336"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHS97_18080"
     CDS             <1..198
                     /locus_tag="VHS97_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450169.1"
                     /translation="SGTTKSGTTKSGTTKSGTIPAKQGDGVSLRDQAQPQFGLIPAAR
                     MMHNDVANTRYNNLGDDDCAA"
     gene            418..1527
                     /locus_tag="VHS97_18085"
     CDS             418..1527
                     /locus_tag="VHS97_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3450170.1"
                     /translation="MAIVLYPHMVVTVPSGYVGVLWKRFGGGTVLDPRKLKNEGFNLI
                     LPWNEVFLYDLRLQSFTESYNAISSDGVSLTATVIVRFRLQRDAVPVLHQAIGPNYVR
                     ILAQPGIGSLTRQVIAQYTAEQVYSTARQEIQDKIRSLVEERLSEKMMEHAGEEGEES
                     YRVSMSDTFILYDILVTGIELPAAIVDAINRKTEQYYIAEEYKFRVEREKRESERKKI
                     EAEGIRDFQQTVSQGISESYLRWRGIEATLQLSQSTNAKVVVIGSGKDGLPIILGNVD
                     SPAPRAGSTPASGNDAMNTERTTAAGPAVPLEKTPAAGLATPAEKIPPAVSGASSAAT
                     TEASRSWWPFSLSGIKAYLFQIVRPTEPKTEARPR"
BASE COUNT          349 a          473 c          501 g          291 t
ORIGIN      
        1 tctggcacga ccaagtctgg cacgaccaag tctggcacga ccaagtctgg cacgatcccg
       61 gccaagcagg gcgatggcgt ttccttgcgc gaccaagcgc aacctcaatt cggcttgata
      121 cccgccgcgc ggatgatgca caatgatgtg gcaaacacgc gttacaacaa tctcggtgac
      181 gatgactgtg ccgcttaaag ggcgacgacg ctataatgac gagtgggacc tgcccgccca
      241 cgaaccgcgc acggaagacg gctttttcgt tgcgcagcgc acggtcgaga acaggcgatg
      301 aaagcgtatc tgtggccagt agacgggatg ccgcccccga ccacgccatc cagatggcgg
      361 cggtttatcg agaactatct gccgatggtc gtgatctact tgatggtcgc gacactcgtg
      421 gcgatcgtgc tctaccctca catggtggta acggttccca gcggctatgt cggcgtgctg
      481 tggaaacgct ttggcggcgg taccgtgctc gacccgcgta agctgaagaa tgagggtttc
      541 aatctcattc tgccgtggaa tgaggtgttt ctctacgacc tgaggctgca atcgtttacc
      601 gagtcctaca acgccatctc cagcgacggc gtgagcctga ctgcaacagt cattgtccgg
      661 ttccggttgc agcgcgacgc ggtccccgtg ctccaccaag cgatcgggcc aaattacgtg
      721 cgaattctgg cgcagcccgg gatcggcagt ctgacgcgtc aagtcattgc tcaatacact
      781 gccgaacagg tgtattcgac ggcgcgccag gaaattcagg acaaaatccg cagccttgtg
      841 gaggagagat tatccgagaa gatgatggag cacgcaggcg aagaaggcga agagtcgtac
      901 cgcgtgtcga tgagtgacac cttcatcctc tacgacatac ttgtgaccgg cattgagctg
      961 cccgcggcca tcgtcgacgc catcaatcgc aaaaccgagc aatactacat cgcggaggaa
     1021 tataagtttc gcgtcgagcg cgaaaaacga gagtcggaac gcaagaagat cgaggcagaa
     1081 ggcatacgcg attttcaaca gaccgtcagc cagggcatct cggagtccta cctgcgttgg
     1141 cgcggcatcg aggcgacgct gcagctttcg caatccacga atgcgaaagt cgtcgtcatc
     1201 ggcagcggca aagacggctt gccgatcatc ctcggcaatg tggatagccc ggcgccgcgc
     1261 gcggggagca cgccggcgag cgggaacgac gcgatgaaca cggaaaggac cacggctgcc
     1321 ggtccggcgg tgccgttgga gaaaacgccc gctgccggat tggcgacccc ggccgaaaag
     1381 ataccacctg ccgtttcggg cgcttcgtct gccgctacca ccgaggcatc gcgctcctgg
     1441 tggccgttca gtctatccgg tatcaaggcc tatcttttcc agatcgtacg tccgacggag
     1501 ccgaagaccg aggctcgccc aaggtagccg tccgagggga cccgtcgcag agcagccgcg
     1561 acggcgcagt tggtggccgt cgtgcagtag cggtcggcgg ttgggagatg gggc
//
LOCUS       DATAQL010001049         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4378141, whole genome shotgun sequence.
ACCESSION   DATAQL010001049 DATAQL010000000
VERSION     DATAQL010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378141"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VHS97_18090"
     CDS             complement(<1..1523)
                     /locus_tag="VHS97_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450171.1"
                     /translation="MKRTLAVAVLIIALGVLVKLTFKQAGPDGGASPGPTHSAPDSGA
                     SEASGRRADPKAELTAKYATEPAQVNELVARVADRFGQNAVAIERTDGLRGLVLLDKL
                     DLEAVFLYEKHPTEFRRLRDFLGGDAAADLLLHWREYFGLKHADDRDRGILIAEISSL
                     TPVQKQIAARYPSALPLILADPAGVTALIDRMPRDDKNLADVLAVLSFVSLEQGSSDL
                     RCALRTFDQHEALALDAFRRHGLDGFAVVSLYGSILEALGTALPLDQGLILLRVNGDY
                     IDELLQTHLPETVAGHLRHVAAVGLTEWVGASPNALRLVVEYGAPGERALRHAGPDAA
                     DVVFGDFSDPTLRRQAVAALAMHGSMALAMLDKYATDPDFRAILRAHGEAVIPPIAQA
                     DAGPRTLAYLGAKNRRTFTESLALAALFASGDNGQATIRTIKNDGLERVAQLNQSEIQ
                     FYQFLPLYDVIHLGNVVRRGYSPTSSEMTWALVDGCFVVADALSLAAVAPEGAVAAES
                     "
     gene            1916..>2379
                     /locus_tag="VHS97_18095"
     CDS             1916..>2379
                     /locus_tag="VHS97_18095"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX3450172.1"
                     /translation="MPHLMSQPPLAPVLLLLWGLFIVYATLLPFDFTATGELIRSRVY
                     RLWERPLRGAGGSLHDAYSNVLLFMPWGFLLAIWRASRGSNWLLAVVWALLSGACLSG
                     FVEIVQLFAPGRYTSFIDLATNTLGAVMGALIGWPLARWIWPIASVRIRQLL"
BASE COUNT          449 a          719 c          711 g          500 t
ORIGIN      
        1 gactcggcgg ccacagctcc ctcgggtgca acggccgcca ggctcaaggc gtcggcgacc
       61 acgaaacagc cgtcgacaag cgcccaggtc atctcgctcg aggtcggtga atagccccgc
      121 cgcaccacgt tgcccaggtg aatcacgtcg tacaacggaa gaaattggta aaactggatc
      181 tcactctgat tcaactgggc gactctctcc agtccatcgt tcttgatcgt ccggatcgtg
      241 gcctgaccgt tatcccccga ggcaaataat gccgccagag ccaacgactc ggtaaacgtc
      301 cgccggtttt tggctccaag ataggccagc gttcggggtc ccgcgtcggc ctgggcgatc
      361 ggcggtatga ccgcttctcc atgggctcta agaatcgcgc ggaaatcggg gtccgtcgca
      421 tacttgtcga gcatcgccag ggccatcgag ccgtgcatcg cgagcgctgc gacagcctgg
      481 cggcgtaacg tgggatcgct gaaatcgccg aagacgacat ccgcggcgtc gggacctgcg
      541 tgtctcaagg cccgctcacc aggtgcaccg tactcgacga cgagccgcag tgcgttggga
      601 ctggcgccga cccattcagt caatccaacc gcggccacat gccgcaggtg gccggcgacg
      661 gtctccggca ggtgcgtttg cagcagctca tcgatgtaat cgccattaac ccgcagcagg
      721 atcaggcctt gatcgagcgg cagggctgtg cccagcgctt ccagaatcga cccatacaaa
      781 ctgaccaccg cgaatccatc gagcccgtgc cgccggaacg cgtcgagggc cagtgcttca
      841 tgttgatcaa acgtccgcaa ggcgcatcgg aggtccgagg acccttgctc aaggctgacg
      901 aagctcagca ccgccagcac gtcggccaga tttttgtcgt cgcgcggcat cctatcgatg
      961 agggcggtca ctcccgcggg atcggccaga atcaagggga gggcactggg ataccgcgct
     1021 gcgatctgct tttggaccgg cgttagactg gagatctcgg cgatcaagat tccccgatcc
     1081 ctgtcgtcgg cgtgtttcaa tccaaaatat tcgcgccaat ggagcaggag atcggcggcc
     1141 gcatcgcccc ccagaaagtc gcgcagccga cgaaattcgg tgggatgctt ttcgtacagg
     1201 aagaccgcct ccaggtcgag cttgtccagc aataccaacc cgcgcagacc atcggtccgt
     1261 tcaatcgcca cggcattttg cccgaatcgg tcggcgaccc gggcgacgag ctcgttaact
     1321 tgtgccggtt cggtcgcgta cttcgcagta agctcggcct tcgggtccgc gcggcgcccc
     1381 gaagcctcgg aggcgcccga gtcaggggcg gaatgcgtcg ggccaggcga cgcaccccca
     1441 tccgggccgg cctgcttgaa ggtcagcttg accagtacac ccagggcgat aatcagcacg
     1501 gctacggcta aggttcgctt catctgatga ttttgtcccg tctacgtgcc gtgcacgaag
     1561 agacggcctg attggtacga gctgaaagcg cttcgctgtt agaaagtaat tcttaccggg
     1621 tcgatcaagc taattgtgaa tccaatgttt tgggcaccgg gattctccag aaccgtgacc
     1681 atatcgtcaa ttcacgttac atcacaccat tcaatcattc accctggtga gcgaagttgt
     1741 aaacgccgag tgcggacggg gtccgttcaa caatcctctg acgtcatgta cattctattg
     1801 cggaattgca cgcactacgg tgattgtgat cggaatgtgc ggtcgcgttg gtccgataaa
     1861 cgtcattttc cccaaacgcg ggattatcgc ggtgctcaag tggatccgat tacgcgtgcc
     1921 acacttgatg tcgcagccac ctcttgcgcc agtgttactg ctgctgtggg gtttgttcat
     1981 cgtctatgcc acgttgctgc cattcgattt caccgccacc ggggaattga tcaggtcgag
     2041 ggtgtatcgg ttgtgggagc ggccgctgcg gggtgcgggt ggatccttgc acgatgctta
     2101 tagcaacgtc cttctcttca tgccgtgggg ctttctgctt gccatctggc gggctagccg
     2161 gggttcgaac tggctgctcg ccgtggtctg ggctctgctg agtggcgcat gtttgagcgg
     2221 attcgtggaa attgtccagt tatttgctcc agggcgttac acgtcattca tcgacctggc
     2281 aaccaacacg cttggcgccg tcatgggtgc cctgatcggt tggcccctgg cgcggtggat
     2341 ctggccgatc gcgtcggtcc gaattcgcca attactctt
//
LOCUS       DATAQL010001050         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1022321, whole genome shotgun sequence.
ACCESSION   DATAQL010001050 DATAQL010000000
VERSION     DATAQL010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022321"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VHS97_18100"
     CDS             complement(<1..368)
                     /locus_tag="VHS97_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450173.1"
                     /translation="MAHLLLIDDDPALIPKQVRQAYPAPSHRVEVARTGALGVDRVRH
                     DPPDVILLDLRLPDQSGLEVYRAIRQIDARIPVIFVTIATSADAAIEAMQQGAFDYLF
                     KPIDLAQLAMAVGAALEVARR"
     gene            879..>2993
                     /locus_tag="VHS97_18105"
     CDS             879..>2993
                     /locus_tag="VHS97_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450174.1"
                     /translation="MRLFDSSGFVPRRLCGEWTAQLILLHNLSDALIWLSYIAIPVVL
                     VYFIRPRRDMPFPSIFWLFGAFIVLCGTTHLMEVVLFYWPHYRLAGLIKLATAIVSVS
                     TVVALVPIVPAALAMRTPGELEREITERRRAEEALRESEERFRGTFENAAVGIAHADD
                     EGRFLRVNAKYCEITGYSREELLAKTFQEITHAEDLPIELEQFGPLMRGELPDYATEK
                     RYVRKDGSHIWINVSLSLQRDAFARPDYAIAILEDISARKRLEAESRLAREAAEAANR
                     AKDEFLANVSHEIRTPMNAILGMTELALDTPLSDDQRQCLETVWSAAESLLCIINDLL
                     DYSKIAAGKLDLDPADFSLRSGLADTLRALAARAHGKGLELVCNVHQDVPDALVGDAG
                     RLRQVVLNLVGNAIKFTEAGEVVMDVDQTGDSYPDKEVELRFVVRDTGIGIPRDKQER
                     IFGAFEQEDTSTTRRYGGTGLGLTIAARLIALMGGKISVQSEPGRGSTFTFTARFGRQ
                     SQHAALAAEQPLDRLQTLTVLVVDDNATNRRILEEWLRAWKMEPTAVGDAAAAMDALW
                     HGLARDRPFGLVLLDSCMPGTSGLSLAAMIRERSELRDTRIILLTSGDPAGDRARSRE
                     LQIDAHLLKPVPQFELLETICRVMSPTTETLKDDGEMLDERKRCEPDLKSSSMPKAPG
                     ARLHILVAEDNEFNKRHLARLLR"
BASE COUNT          584 a          933 c          924 g          552 t
ORIGIN      
        1 cgccgggcca cttccaacgc cgcgccgacg gccatcgcca attgggccag atcgatgggt
       61 ttaaataggt agtcgaaggc gccttgctgc atcgcctcga tggccgcgtc cgccgacgtc
      121 gcaatggtga cgaagatgac aggaatgcgt gcgtcgatct gccgaattgc ccggtagacc
      181 tccagtcccg actggtcggg caggcgcaag tcgagaagta tcacgtcggg tgggtcgtga
      241 cgaacgcgat cgaccccgag tgcacccgtg cgggcgacct cgacacgatg cgagggggca
      301 gggtacgctt gccgcacttg cttcgggatc agggctgggt cgtcatcgat tagcaagagg
      361 tgagccatgt tgttcgctcc agggccacca aaaacgacgg tgatcgaaca accaagccga
      421 cgaatggcca gagcttgcga cagaccgtgg actcttcgcc tgccggtttg gcatttttat
      481 tgcaaacgca ccagcttggc acaagcggcg atcgtcgtga ttccggccgc gactgtgatg
      541 gcggcaacga attgcgagac acccaacatc agccggagac gaccattcgc tatcgttgcg
      601 gcttcctgcg gcaacacggc agacttccgg gccgctgggg tgagaaatca tcggggtggc
      661 cgactcgtgc ctttgcaagg ggccaagggc cttggtgagc gaccgagtac atcacggttt
      721 gcggtagaaa cgagttggtg cgtcgctccg gactgttcct ctcggttggt cgttcatgac
      781 gggtccggga gcagtccttg tcccaaaaaa attgtgcagc cgcgctatct taccgccaag
      841 aaccacggtt accaaggggg tgtccggtgg attttgtcat gagactattc gactcatccg
      901 gttttgtgcc gcgacggctt tgcggcgagt ggaccgccca gctcattctg ctgcacaacc
      961 tctcggacgc gctcatctgg ctgtcgtata ttgcgatccc cgtggtgctc gtttatttca
     1021 tccgccccag gcgcgacatg ccgtttccgt cgattttctg gctttttgga gcttttatcg
     1081 tcttgtgtgg aaccacccac ctgatggagg tggtactctt ttactggccc cactaccgac
     1141 tggccggcct gatcaagctt gccacggcaa tcgtttcggt cagcacggtc gtcgctttgg
     1201 tgccgatagt ccccgcagcc ctggccatgc gcacgcctgg cgagctggag cgcgaaatca
     1261 ccgagcgcag acgcgccgag gaggcattgc gcgagagcga agagcggttc cgcggcacgt
     1321 tcgaaaacgc agccgtcggc atcgcccacg cggatgacga aggccgtttc ttgcgcgtca
     1381 acgccaagta ctgcgaaatc accggctaca gccgcgaaga gctgctcgcc aagacctttc
     1441 aggagatcac tcatgccgag gacctgccaa tcgagctcga gcaatttggt ccgctcatgc
     1501 gcggcgaact gccggactac gcgaccgaga aaaggtatgt ccgcaaagat ggttcgcata
     1561 tctggatcaa tgtgtccctc tcgctccagc gcgacgcttt cgccaggccg gattacgcca
     1621 tcgctatcct ggaagacatc tccgcacgca aacgcctgga agccgagtct cgactcgcca
     1681 gagaggcggc cgaggcggcc aatcgagcca aggacgagtt tctggccaat gtcagccacg
     1741 agatccgaac gccgatgaac gccatcctgg gaatgaccga gctggcgctc gatacgccgc
     1801 tcagcgacga ccagcgacaa tgtctggaga cggtatggtc ggctgcggag agcctccttt
     1861 gcatcatcaa cgacttgctc gattattcca agatcgcggc tggaaagctc gacctcgacc
     1921 cagccgattt ttcgttgcgg tcgggcctgg cagacacact gcgcgccctc gcagcgcggg
     1981 cccacggcaa ggggctggag ctggtctgca atgtgcacca ggacgtaccc gacgccctgg
     2041 tcggcgacgc cggccggctg cgacaggtgg tgctcaactt agtcggcaac gccatcaagt
     2101 tcaccgaagc gggcgaggtg gtgatggacg tcgaccagac cggcgattcc tacccagaca
     2161 aggaagtcga gctgcgcttc gtggtgcgcg acaccggcat cggcatcccg cgcgacaagc
     2221 aagagcggat cttcggtgcc ttcgagcagg aagacacttc gaccacgcgg cgctatgggg
     2281 gcaccgggct aggcctgaca atcgccgctc ggctaatcgc cctgatgggc ggcaagatca
     2341 gcgttcagag cgagccgggc cgaggcagca ccttcacatt cacggcgcgg ttcggacggc
     2401 agtcgcaaca cgcggcgctc gccgccgagc aaccgctgga ccggttgcag accttgacgg
     2461 tcctcgtcgt cgatgataac gccaccaatc gccgcatcct cgaagagtgg ctgcgcgctt
     2521 ggaagatgga gcccaccgcg gtgggcgacg cggcggcggc catggacgcg ctctggcatg
     2581 gcctcgcccg ggatcgaccc ttcggcctgg tgcttctgga cagttgcatg cctggcacgt
     2641 cgggcctgtc gttggccgct atgattcgcg agcgctctga actgcgcgac acccgaatca
     2701 tcctcttgac ctccggagac cccgccggcg atcgcgctcg atcacgcgag ctgcagatcg
     2761 acgcccactt gctcaagccc gtcccacaat tcgagctcct ggagacgatt tgccgcgtga
     2821 tgagcccaac aacagagacc ctgaaggatg acggcgaaat gctcgacgaa cgaaaacgat
     2881 gcgagccgga tctgaaatca tcatccatgc ccaaggcgcc tggcgcgcgc ttgcacattc
     2941 ttgtggcgga ggacaacgaa ttcaacaagc gccacctggc tcgactgctg cgc
//
LOCUS       DATAQL010001051         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3923594, whole genome shotgun sequence.
ACCESSION   DATAQL010001051 DATAQL010000000
VERSION     DATAQL010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3923594"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VHS97_18110"
     CDS             complement(<1..657)
                     /locus_tag="VHS97_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3450175.1"
                     /translation="MRRFLRHLVVGSAVVFLDPLAHAWGQTDSKLEPAPSGFASRRDG
                     LERGTLETVEYDSKSVGDTRKMTVYLPPGYSKDTKYPVFYLLHGAGDNEGGWTRTGAA
                     NVILDNLFADKKIVPMIVVMPNGSPQGPRPGTVLAGTIMKRADADKNGIVSREEFLAA
                     ALALYKDLDTGQLDEKQLAAGLNRLMPASGAPGGRQRGFGDFVSGFENDLMKDLIPYV
                     E"
     gene            1024..1542
                     /locus_tag="VHS97_18115"
     CDS             1024..1542
                     /locus_tag="VHS97_18115"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3450176.1"
                     /translation="MAELGVHQSALLTDLALCVNRYRLFSPGWYVCVLAKVQLSPEES
                     EVPGLVAMVNYGRKKQCEVRDEVFHGAPNFVLDVFYSEDDQDFLRRRDRFCNFGVHEY
                     LVAFDAEPVGLLWHRLDAGCYRLVEPDEDGIIRSNALPNLWLPLRAVQDRDWWAVLGC
                     IERGVSRRANFA"
BASE COUNT          374 a          546 c          599 g          392 t
ORIGIN      
        1 ttcgacatac gggatgagat ccttcatcaa gtcgttttca aagccgctga cgaagtcgcc
       61 gaacccgcgc tgacggccac ccggagcgcc ggaggccggc atgagacgat tgaggccggc
      121 ggcgagctgt ttctcgtcca gttggccggt atcgaggtcc ttgtagaggg ccagggcggc
      181 ggcgaggaac tcttcccgag agacgatgcc gttcttgtcg gcatctgctc gcttcatgat
      241 cgtgcccgca aggaccgtgc ccggccttgg cccctgggga gagccgttgg gcatgaccac
      301 gatcatgggg acgatcttct tgtcggcgaa gagattgtcg aggatgacgt tggctgcacc
      361 ggtcctggtc cagccccctt cgttatcacc ggcgccgtgc agcagataga agaccgggta
      421 tttggtgtct ttcgagtagc cgggcggcag gtagaccgtc atcttgcgtg tgtcgccgac
      481 cgatttcgag tcgtactcca cggtctcaag cgtgccgcgc tcgagaccgt cgcgccggct
      541 ggcgaagccg ctgggtgccg gttcgagttt cgagtccgtt tgcccccacg cgtgtgccag
      601 cggatccagg aaaacaacag cgctcccgac cacgaggtgg cgcaggaatc gtctcatcgg
      661 gatctcctcc acaacaaagg caacgatgga caccagatac ccaggcgcga cgcgcagctc
      721 acccaggacg gcctagcccc gtgccgctgc gcccgccacg cgccgtccgc gatcgaaccg
      781 aagactccgt gcttcaagct acgctccgtg gctgtcgtcg acggaaaggg ccgctctcac
      841 gccgaggatc gcgcagcgga ggatccctca ttatgaagaa gcgacgtttc acgtcgcgtc
      901 gtcccggtcc catgccactc tttgcgaaga tgaaaaccta taccgcacaa ttgcggtcta
      961 caggtaatat gtaaaaacta tctaaaagca agttcggcgg ctcttcgacg ttggagcggc
     1021 gaaatggctg aactcggagt ccatcaatcc gcgctgctga cggacctggc tttgtgcgtc
     1081 aaccggtatc gcttgttctc gccgggttgg tacgtgtgcg ttctggcaaa ggtccaatta
     1141 tccccggagg aatcggaggt tcccggattg gtggcaatgg tgaactatgg acggaagaaa
     1201 cagtgtgagg tgcgcgacga ggtatttcac ggagcaccga atttcgtgct cgacgtgttt
     1261 tactcggaag acgatcagga ctttctgcgt cgccgcgatc ggttctgcaa tttcggcgta
     1321 cacgagtacc tcgtcgcttt cgacgccgaa ccggtaggcc tgctttggca tcgattggat
     1381 gcgggttgtt accggttagt cgagcccgat gaggacggta tcatcagaag caatgcgttg
     1441 ccgaatctgt ggctcccact gagagcggtt caggatcgcg attggtgggc ggtgctcggt
     1501 tgcattgaaa ggggcgtgtc acgtcgtgca aatttcgcct gacacgttct ggcgcagatg
     1561 ccgacagtgg actgccgccg aaccaaggcg acaggaaagg cgacatgcat tgcacgtacg
     1621 tcgccgcggc tcgatgaata ggcacgataa gcaaagcacg aaagaatcgg cggcgccgcg
     1681 atccgcaacg gattaagacc ggtcgacaga ccgtttgtaa cgctgctggg caccgcgcct
     1741 aaagggtcaa tggggcggtt ttgataggac cctagcccgg gcgtgttcgc acacgcgttg
     1801 ccggtgccaa gtagacgtag gatatgggtg aagagcggct acacttccgt gctcatcaac
     1861 agttgccttt ccgtctttca tcggtagccg gatccgtcgc catcggcgtt t
//
LOCUS       DATAQL010001052         7683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2702785, whole genome shotgun sequence.
ACCESSION   DATAQL010001052 DATAQL010000000
VERSION     DATAQL010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2702785"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /locus_tag="VHS97_18120"
     CDS             <1..1744
                     /locus_tag="VHS97_18120"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX3450177.1"
                     /translation="AVEHEVTELRGWLQTELPAYMVPSAFVVLQALPLSSNGKIDRKA
                     LPAPGPGQLDPVVEYVAPRDPLEETLVRVWAEVLELERVGVHDNFFDLGGHSLQSVQL
                     VARLTAALNRPVSVKTVFQAPTVAAMADLLKREAAACGSEDRAHRSDDEPLASVPWIL
                     ESEPAALPEHVTIERRPFASLFAAGELAPVESVALGYLPSALLPFTGLSRATVIHDWC
                     GNRPLITDVRETPLGRIGSVVIPRFDDELYLNRQDLLAVLGDAVRLAHEIGAATVSLT
                     GLLPSASGYGRDLAECLAGHDVPRITTGHATTTSAVVLAVRRGLEEAGRALADEHVGF
                     IGLGSVGLATLRLLLSCLPHPARLSLCDVYSKWESLESLRRELIDELGYRGEVRLLAS
                     RHEVPAEFYEARVIVGATNVANILDIDRVAAGSIVVDDSAPHAFDSEKALGRFHKRGD
                     ILVTEGGVLLAPEPLPLRVFVPDELEPWLKAGLVSLIARSNPWNITGCVLSGLLSARF
                     ANLAPTIGLIDRQTALDHYKTLDALGFSAAGLQLDDSPLDANIIQEFRSRYSKCHRAG
                     TPNGNGPDSSVSQR"
     gene            1741..3387
                     /locus_tag="VHS97_18125"
     CDS             1741..3387
                     /locus_tag="VHS97_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HEX3450178.1"
                     /translation="MSLIRLLVRSSRSIVILSALTGAAGGVAGIALIALIQQELATES
                     VSGTLAGAFFALCVVSAVARAIAQIAMVKIGQGAIAELSLRLVRDILVLPLRAFETID
                     NSALLSALTDDIALIANAMVGIPHLCVNIPIVIACLAYTGWLAPRTMACGVVFAALAI
                     GAYVFVSARGMKSLRRARERQALLVGHFRTLIGGFRELKVHRGRREAYLAESLEPTMA
                     SARAEMVSGMAHFAVAEGWGQIAYFGFIGLLLYAAPLIEPISRPALVSAVLVVLYLMT
                     PLDIILTWVPVLGRAQVSLQRVQALIPTLERDGDDNDSRPVPIKPLAIRDSICLEGVT
                     FTYREGDDDAGFLLGPVDLTLRPGELVVLSGGNGSGKTTLVKLVAGLYQPESGEVRID
                     GHRLGDEDREAYHQLFSIVFADGHVFRDFFGLGADRIAKLARDGLERLGLASVVSVRG
                     STFSTLDLSMGQRRRLALLGALLEDRPVCIFDEWAANQDLSFKQIFYHKLLPELRAAG
                     KALLVISHDESHFDIADRVIRIQDGRVFEESPLGIGRAWA"
     gene            3411..4679
                     /locus_tag="VHS97_18130"
     CDS             3411..4679
                     /locus_tag="VHS97_18130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450179.1"
                     /translation="MKKLLLLIILFWGAGAYGIWYWTDPRTQRVSYRAVSIKRGDLSS
                     TINTTGTIEPEEVVDVGAQVAGRVESFGSDPGDPSKTVGYGSRVERGTILARLDSALF
                     QSRLDQERGRVAKAEADIEQARAKLRQADRELERCRKLKARDTVVIAAQEYDAATANF
                     ESAKATLAVAQAALAVARADLEEATVNLEYTTIRSPVKGIVLDRRINIGQIVVASLST
                     PSLFLIAKDLSRLEIWSSVNETDIGSIHVGQKVHFTVSSLPRDTFLGEVSQIRLNASM
                     TQNVVTYTVVIAVDNSSGRLLPYLTARLQFEVETRKGVLLVPNAALRWQPSAQNIIAE
                     ARDSHELALHRRTAASSSNEPIGLLWIRQGDLVRPIKVRIGLSDGLVTEVSGDGLTEG
                     TEIVVGANRIDSDPDALSILPHTWSEPSKK"
     gene            4726..6930
                     /locus_tag="VHS97_18135"
     CDS             4726..6930
                     /locus_tag="VHS97_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3450180.1"
                     /translation="MEFIRLEDIHKVFTRGTIELPVLRGVSLSIARREMVALMGASGS
                     GKTTLINLMGFLDRPTSGRLWLDRHDVTRLGETERAWMRSREIGFVFQNFNLLPRLTA
                     LENVMMPSVYRSHDLSEHECRARARALLERVGLRDRFDHEPSRLSGGEQQRVAIARAL
                     INRCSLLIADEPTGNLDSKTGEEILGLFRQLNREDGLTVVLVTHDPTVAAHADRIIRI
                     RDGLVFDDDTFVDQPPNQRTGAESGEIEPADRFFRPMNPPRFDADDPTLNRRTMRDDM
                     RFFARTTSTALRSLRRNALRSALTTLGIIIGVGSLLAIAEIGKGAWSAIRILLTKTGV
                     DNIVIQAGAASRNGISLGSGSIKTLTPEDAETILHECPSVDSLAPLVFTRRQVVHGNK
                     NWVPSAFIGTTPGYLRVREWEDLDQGGPFTDQDVHDVAMVCLLGQTVAHELFGEQSPV
                     GQEVYANDVPLRVVGVLSRKGADIIGEDQDDLLVAPWTTVKFRVSAGAASSSEGKDAL
                     APDPIALLSPLARRYPRRQVAPFPTVSTMQSIDTPRLERLSNIDSILVRALSTEEIPA
                     AMDQITRVLRERHRIAPGETADFDVRDFTEVVHAVKGTVGLVARLLLCVALISLLVGG
                     IGIMNIMLLTVTERYKEIGLRMAVGARSRDILRQFLVEAVVLCVLGGAVGIAAGRGAS
                     VLVRLLARWPTEASSIAVLASVSVSITVGVIFGYYPAWKASRLDPIEALRFE"
     gene            7125..7628
                     /locus_tag="VHS97_18140"
     CDS             7125..7628
                     /locus_tag="VHS97_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450181.1"
                     /translation="MMRIRSKRQRSVGFEALEGRLVLSAGAGTAAASHHAVPAVAKLT
                     QKSIPAAFKGHVQIVSASELMVTGLKGTIGTDHFTGSGTGTAVGKQFGGGVVNLSNSQ
                     GSVQLQLSPAFTVKVGRSSKQEVSMVAVNGSGKYVSYDGITGTITTWNVPAKPSASAS
                     FSGSFKP"
BASE COUNT         1345 a         2463 c         2392 g         1483 t
ORIGIN      
        1 agcggtcgag cacgaagtga ccgagctgcg cggctggtta cagacggagc tgcccgctta
       61 catggtcccc tcggcgtttg tcgtcctcca ggccctgccc ctctcctcca acggcaagat
      121 cgaccgcaag gcgttgccgg cccccggtcc cggacaactc gacccggttg tggagtacgt
      181 ggcgccgcgc gatccgctcg aagaaacgct cgtccgcgtc tgggccgaag tactggagct
      241 cgagcgggtc ggggtgcacg acaatttctt tgacctcggc ggccactcgc tccagtccgt
      301 gcaactcgta gcacgtctca ccgcggcctt gaatcggccg gtttcggtca agacagtttt
      361 tcaggcgccc acggtcgccg cgatggcaga tctcttgaag cgcgaggcgg ccgcgtgcgg
      421 ttccgaagat cgagcgcatc gcagcgacga cgagcctctt gcgtcggtcc cgtggatact
      481 cgaatcggag ccggccgcgc tccccgagca cgtgaccatc gagcggcggc ccttcgcctc
      541 gcttttcgcc gccggggagc tcgcgccggt tgagtcggtc gccttgggat atctaccgtc
      601 cgcgctcctg ccctttacgg gtctcagccg cgcgacggtg atccacgact ggtgcggcaa
      661 caggccgttg atcaccgatg tccgggagac tccgctgggc cggatcggct cggtggtgat
      721 cccccgcttc gacgatgagc tttacctgaa ccggcaagac ctcctggccg tcctcgggga
      781 tgccgtccgg ctcgcgcatg agatcggcgc ggcgacggtg tcactcacgg gattgctccc
      841 ctcggccagc ggctacggcc gggacctggc cgagtgcttg gccggccacg acgttccgcg
      901 gatcaccacc ggccatgcca ccacgacatc cgccgtagtg ctggccgtcc gtaggggcct
      961 ggaggaggct ggtcgcgctc tggccgacga gcacgtcggc ttcatcggcc tgggatcggt
     1021 tggcctcgcg acgctccggc tcttgctttc ttgcctgccg catcccgcac ggctcagcct
     1081 gtgcgacgtc tattcaaagt gggagagcct cgagtcgctt cggcgcgagc tgatcgacga
     1141 attgggctat cgcggtgagg tgcggctgct cgcgtcgcgg cacgaggtgc ctgcggagtt
     1201 ctatgaggcg cgcgtgatcg tcggggcgac gaacgtcgca aatatcctcg acattgaccg
     1261 cgtggccgcc gggtcgatcg tggttgacga ctccgctccc cacgcctttg attcggagaa
     1321 ggcgcttggg cgtttccaca agaggggcga catccttgtg accgaggggg gtgtgctcct
     1381 ggctccagag ccgctcccac tccgggtctt cgtccccgat gaactcgagc cttggctcaa
     1441 ggccggcctg gtctcgctca tagcgcggag caatccgtgg aacatcaccg gctgcgtgct
     1501 ttccggcctg ctttcagcca ggttcgccaa tttggcgccc acgatcggac tcatcgatcg
     1561 gcagacggca ctggaccact acaagacgct cgacgcgctt ggctttagcg cagcgggcct
     1621 ccagctcgat gattcgccgc tcgacgcgaa catcatccag gaattccgct cgcggtatag
     1681 caagtgtcat cgcgccggta ccccgaatgg gaacggcccc gacagttcag tgagtcaacg
     1741 atgagcctta taaggttgct ggtgcggtcc tcgcggagta tcgtgattct ctcggcgctc
     1801 accggggcgg ccggcggggt ggccggcatc gcgctcatcg cgctgatcca gcaagagctg
     1861 gcgaccgagt cggtctcggg caccctggcc ggggcgttct tcgcgctctg cgtggtgtcg
     1921 gccgtggcgc gcgccatcgc ccagatcgcg atggtcaaga tcgggcaagg ggcgatcgcg
     1981 gaactcagtc tgcgcctcgt ccgcgatatc ctggtgctgc ctctgcgggc gttcgagacg
     2041 atcgacaatt cggccctcct gtcggctctg accgatgaca ttgccctgat cgccaatgcc
     2101 atggttggga tcccgcacct ctgcgtcaac atcccgatcg tgatcgcctg cctggcttat
     2161 acgggctggc tcgcgccgcg gaccatggcg tgcggtgtag tgttcgccgc cctggcgatc
     2221 ggcgcctacg tgtttgtgtc agcgagaggc atgaagtcgc tgcggcgcgc ccgcgagcgg
     2281 caggccctgc tggtcggcca tttccgcacc ctgatcggcg gatttcggga gctcaaggtc
     2341 caccgagggc gccgcgaggc gtacctcgcc gagtcgctcg agccgaccat ggcctctgcc
     2401 cgcgcggaga tggtcagtgg catggcccac ttcgccgtcg ccgaaggctg gggccagatt
     2461 gcctacttcg gctttatcgg cttgctcctc tacgccgctc ctttgatcga gccgatatcg
     2521 cggcccgctc tcgtctcggc cgtactggtc gtcctctatc tgatgacccc tctagacatc
     2581 atcttgacgt gggtgcccgt cctgggccgg gcgcaggtct cgctccagag agttcaggcc
     2641 ttgatcccca ctctcgagcg ggacggggac gataatgaca gccggccggt accgatcaag
     2701 ccgctggcga tccgcgactc gatttgcctc gaaggcgtga cgttcactta tcgcgagggc
     2761 gacgatgacg cgggctttct gctcggccct gtcgacctga ccctccggcc gggcgagctg
     2821 gtcgtcctgt ccgggggcaa cggcagcggc aagacaaccc tggtcaaact ggtcgccggt
     2881 ctctaccagc ccgagtccgg cgaagtgcgc atcgatggcc atcgactcgg cgacgaggat
     2941 cgcgaggcct atcaccagct cttctcgatc gttttcgctg acggccatgt cttccgcgac
     3001 ttcttcggct tgggcgcgga caggatcgcg aaactggctc gcgacggtct cgaacgactg
     3061 ggtttggcgt cggtagtttc ggtccgaggg agcacattct cgaccctcga cctgtcgatg
     3121 gggcaacgac ggcgactggc cctgctgggc gcgttgttgg aggatcggcc cgtctgcatc
     3181 ttcgacgaat gggccgcgaa tcaggacctt tcgttcaagc agatttttta tcacaagctg
     3241 cttcccgagc tgagggccgc gggaaaggca cttctggtta tcagccacga cgagagccac
     3301 ttcgacattg ccgaccgcgt cattcgcatc caggacggcc gcgtcttcga agagtcgccg
     3361 ctgggtatcg gtcgcgcgtg ggcctgaggg acttagaaaa ggatcgcatc atgaagaaac
     3421 ttcttttatt gattattctc ttctggggcg cgggcgccta cggaatctgg tactggactg
     3481 atccgcgcac ccagcgcgtg tcgtatcgtg ccgtctccat caagcgaggc gacctcagtt
     3541 ccacaatcaa cacaaccggg acgatcgagc ctgaagaggt cgttgacgtg ggcgctcaag
     3601 tggccggccg ggtcgagagc ttcggctccg accctggcga cccgagcaag acggtcggct
     3661 acggctcgcg ggtcgagcgg gggacaatcc tggcccgcct cgacagcgcg ctgttccagt
     3721 cgcgcctcga ccaggagcgt ggccgagtcg caaaggccga ggctgacatc gagcaggctc
     3781 gggccaagct ccggcaggca gaccgcgagc tcgagcggtg ccgcaaactg aaggcacgag
     3841 ataccgtcgt catcgccgcc caggaatacg acgcggctac ggcaaacttc gaatcggcga
     3901 aggccacgct ggccgtcgct caagccgccc tggctgtcgc cagggctgat ctcgaagagg
     3961 ccaccgttaa cctcgaatac accacgatcc gctccccagt caaagggatc gttctcgacc
     4021 ggcggatcaa tatcggtcag atcgtcgttg ctagcctgag tactccgagc ctcttcctca
     4081 tcgccaaaga cctgagccgc cttgagatct ggtcgtcagt caacgagacc gacatcggct
     4141 cgatccacgt cggtcagaaa gtccacttca cggtcagctc gctgccacgc gacacgttcc
     4201 ttggcgaggt ctcgcagatc cgcttgaacg cgagcatgac acaaaacgtg gttacttaca
     4261 ccgtcgtcat cgcggtcgac aactcaagtg gccgtctttt gccgtacctg accgcgcgcc
     4321 tccagttcga ggtcgagacg cggaaggggg tcctgctagt gcccaacgcc gctctccgtt
     4381 ggcagccaag cgcgcagaat atcatcgctg aggcacgcga cagccacgag ctggcgcttc
     4441 accgacggac ggctgcctcc tcttcaaacg agccaatagg actcctctgg atccggcagg
     4501 gcgatctcgt ccggccgatc aaggtgcgca tcggcctctc cgacggtctc gtcaccgagg
     4561 tctcgggaga cgggttgacc gagggcacgg agatcgtcgt cggtgcgaac cggatcgatt
     4621 ccgatcccga tgccctgtct atcttgccgc atacctggag tgagccttcg aagaaatagg
     4681 cgtcacacca atcacgacct gtgccgcgaa actggagact cgatcgtgga gttcatcagg
     4741 ctggaagata tccacaaagt ttttacgcgg gggacgatcg agttgcccgt gctccggggt
     4801 gtctcgctct cgatcgcgcg gcgcgagatg gtcgccctca tgggtgcctc gggttcggga
     4861 aagacgacac tcatcaacct catgggattc ctcgaccgcc cgacgagcgg tcggctctgg
     4921 ctcgatcggc atgacgtcac gagactcggc gaaacggaac gagcctggat gcggagccgg
     4981 gagatcggat ttgtctttca aaactttaac ctcctccctc gattgacagc cctcgagaac
     5041 gtgatgatgc cgtcggtgta ccggtcgcat gatctttccg agcatgagtg ccgtgctcgg
     5101 gcgcgggcgc tcctcgagcg ggtcggcctg cgcgatcggt tcgaccacga gccatcacgg
     5161 ctctcaggtg gcgagcagca acgcgttgca atcgcccgcg cgctcatcaa ccgctgctcc
     5221 ctattgatcg ccgatgagcc aaccggcaac ctcgattcga agacgggaga ggagattctc
     5281 ggcctcttcc gccagctcaa ccgcgaggac ggtctcaccg tcgtcctcgt gacacacgac
     5341 cccaccgtcg ccgcacacgc cgatcgtatt attcgcatac gcgatgggct cgtcttcgat
     5401 gacgacacgt tcgtcgacca accacccaac caacggactg gagccgagag cggcgagatc
     5461 gaaccggcag atagattttt ccgaccgatg aatccgccgc gtttcgacgc cgatgacccg
     5521 accctaaatc gaagaaccat gcgcgatgat atgcggttct tcgcccgcac gacctccacg
     5581 gccttgcgtt cactccggcg caatgccctg cgttccgcac tgacaacgct cggcatcatc
     5641 atcggggtcg gatcgctcct tgccatcgcc gagatcggca agggggcatg gtcagcgatt
     5701 cgcatactct tgaccaagac gggtgtcgat aacatcgtga tccaggccgg cgccgcgtca
     5761 cgcaatggca tcagtctcgg cagcggcagc atcaagaccc tgacgcccga ggacgcggag
     5821 acaatcctcc acgaatgccc gagcgtcgac agcctggcac ccctggtctt tactcgccgg
     5881 caagtggtcc atgggaacaa gaactgggtt ccgagcgcct tcattggcac caccccggga
     5941 taccttcgcg tcagggagtg ggaagacctg gatcagggtg ggccgttcac cgaccaagac
     6001 gttcatgacg tggcgatggt ctgcctgctc ggccagacag tcgcgcacga gctcttcggc
     6061 gagcagtcgc cggtcggcca ggaggtttac gccaacgacg tgcccctccg ggtcgtcggc
     6121 gtcctgagcc ggaaaggggc cgatatcatt ggcgaagatc aagacgacct gctcgtcgcg
     6181 ccctggacga cggtcaagtt tcgggtgagc gcaggggcgg cttcctccag cgaaggcaaa
     6241 gacgcgctcg cgccggatcc cattgcgcta ctcagtccgt tagcgcggcg ctacccccgc
     6301 cgacaggttg ctccgtttcc gaccgtctct acgatgcagt cgatcgacac accccggctg
     6361 gagcggctat ccaacattga ctcgatcctg gtccgggcgt tatcgaccga ggagatcccg
     6421 gctgcgatgg accagatcac tcgtgtgctc cgcgaacgtc accgaatcgc accgggagag
     6481 accgccgatt tcgacgttcg cgacttcacc gaggtcgtcc atgccgtcaa gggaaccgtg
     6541 ggccttgtcg cgcggttgct actctgcgtc gccctgatct cgctcttggt cggcgggatc
     6601 gggatcatga acattatgct cctaaccgtt acggagcgct acaaggagat aggcctgcgc
     6661 atggccgtcg gcgctcggtc ccgggacata ctccgacagt tcctcgtcga ggctgttgtg
     6721 ctctgtgttc ttgggggagc agtcggtatc gcggccggcc ggggagcctc ggttctcgtt
     6781 cgtctgctgg ctcgctggcc aaccgaagcc tcttctatcg ccgttctggc gtctgtctcg
     6841 gtttccatca ccgtcggcgt gattttcggc tattacccgg cttggaaggc ctcacgcctc
     6901 gacccgatcg aggccctccg gttcgagtga catcattctg cgtccggacg ggtggcctgg
     6961 ccatgtgagc gttctcattc gatgattcca acgtcaatct cgttccgaaa acctacttac
     7021 tggagttaac cccgaagccg gaagcgaagt ggcgcggtcg tcccggcgag aatactgtca
     7081 gcgctacttg tgaacataac ttctttgatt tggagcaacc gatcatgatg cgaattcgtt
     7141 caaaacgcca gcggtccgtg gggttcgagg cgctcgaggg aagattggta ttgagcgcgg
     7201 gcgcggggac ggccgcggcc tcacatcatg ccgtgccggc agtggcaaaa ctgacccaga
     7261 aatcgattcc ggctgcgttc aaaggccacg tgcagatcgt tagcgcttcc gagctgatgg
     7321 tcacgggtct taagggaaca atcggcacgg accatttcac tggatctggc actggaacgg
     7381 cagtgggcaa gcaattcgga ggaggagtcg tcaacctgag caacagtcag ggtagcgttc
     7441 agctccagct cagccctgcc tttacagtaa aggttggaag gtcctcaaag caggaagtct
     7501 caatggttgc cgtaaacggc agtggaaaat acgtctcgta cgacggcatc acgggaacga
     7561 tcacgacatg gaacgtccca gccaagccaa gcgcatcggc aagcttttca ggatctttca
     7621 aaccctgatg cctagccgcc ggtgaagggc tcggcgactg acggactcga ggcggcgcaa
     7681 gga
//
LOCUS       DATAQL010001053         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3923999, whole genome shotgun sequence.
ACCESSION   DATAQL010001053 DATAQL010000000
VERSION     DATAQL010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3923999"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            549..>1587
                     /locus_tag="VHS97_18145"
     CDS             549..>1587
                     /locus_tag="VHS97_18145"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEX3450182.1"
                     /translation="MGTRITLANWEQYKAFMPEGMIALFSGRYGLKMPTDVEIDVGPP
                     RHIVAPSTFRRATEQHEGQVRVVHLADGHNDIANYVAGEPFPSPARPDKGYEILADLW
                     YTYSPHLAVGMPGQGPWNVTMLDRFGNATSASWAFVYRQLAFNTDPGVEAIDPRAANA
                     FYSQWLMTEEPEQAKYTTDLDILPLDNQREESNYLFIPALRHSMRLSLGARCAPLLGT
                     DWTHDDQKPGFNGGLASFDASYLRDMKMLALVDLSSAVADFPSQYDMPLGFARPSWGK
                     WSQRDAWVIDIHRIPTESLGYCYAKRIEYVDKETTRALWEDLYDSAKQLWKITMNAYC
                     PSRVPRTDGDTL"
BASE COUNT          335 a          513 c          460 g          279 t
ORIGIN      
        1 cctcgctagg gagtttaagc gctcagacgt ccaccacgcc ggcttcgacg ccgaaaatca
       61 ttaccgaacc agagccttct ctcgccgctc tggtttcgca acaggtcgct tccgaaaaaa
      121 gcccggccgg cgggccgtca cccgaatcgc tggtctcatc tccaaatcgc catcgagagg
      181 tcgcgcctga cgcgatcgaa gctgccgtgg cgggtattcg caaattgacg tcggcagcgc
      241 cctcgatgct ggcgcagatg ccgccacgac caatcaggac gcggacaccg ctcccgctca
      301 cgcgtcattc gaacgttacg ccgtccgctt tggaaaacgc ggagcaagat acggaaccca
      361 ccagcaccat gcggccgacg ccaaaagtcg tcgccagcgc agagcgagaa acaacgccga
      421 tcagtgacga tgccgcgagg cccaatgtag catcgccggt tcggatctcc aggatttatc
      481 ctcctcatcc ggaacgcgcg gtcgcgcccg ccttgccccg tccacaggat gacagcggag
      541 ccatttcagt gggcaccagg atcacccttg ctaactggga gcagtacaag gccttcatgc
      601 ccgagggcat gatcgcgctc ttctctggcc gctacgggtt gaaaatgccg accgacgttg
      661 agatcgacgt cggaccgccg cgccacatcg tcgcgccgag cacattccgg cgagcgaccg
      721 agcagcacga gggacaggtg cgggtggtgc atctcgccga cggccacaac gacatcgcca
      781 actatgttgc aggcgaaccc tttccgagtc ccgcgcgacc cgacaaggga tacgagattc
      841 tcgccgacct gtggtacacg tatagcccgc atctggcggt cgggatgccg ggacaggggc
      901 cgtggaacgt tacgatgctc gaccgcttcg gaaatgccac ctctgcaagc tgggccttcg
      961 tttatcggca gctcgccttc aataccgatc ccggcgtcga ggcgatcgat ccccgagcgg
     1021 ccaacgcctt ctatagccag tggttgatga ccgaggagcc ggagcaggcc aagtacacaa
     1081 ccgatctcga tatcttgccg ctggataatc agcgggagga gtcgaactat ctattcattc
     1141 cagcactcag gcactcgatg aggctgtcgc tcggcgcccg ctgcgcgccc ttgctcggga
     1201 ccgactggac gcatgacgat cagaagcccg gcttcaatgg cggcctcgcg agtttcgatg
     1261 ccagctacct gcgtgacatg aagatgcttg ctctggttga cctcagctcc gccgtggcgg
     1321 atttcccgtc ccagtatgac atgccgctgg ggttcgcacg gcccagttgg ggcaaatgga
     1381 gccaaagaga cgcctgggtg atcgatattc accgtatccc aacggaaagc ctcgggtatt
     1441 gctacgccaa acggatcgaa tacgtcgaca aggagactac gcgcgcactt tgggaagacc
     1501 tctacgattc tgcaaaacag ctttggaaaa tcacgatgaa cgcgtactgc ccgagcaggg
     1561 tgcccaggac cgacggcgac acgctct
//
LOCUS       DATAQL010001054         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3313598, whole genome shotgun sequence.
ACCESSION   DATAQL010001054 DATAQL010000000
VERSION     DATAQL010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313598"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VHS97_18150"
     CDS             <1..243
                     /locus_tag="VHS97_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX3450183.1"
                     /translation="NRIGHEGPAGGGLEFWGGSFVADPFGRVIAKAGPGTEEVVIARC
                     DPKLQEETRRNWPFLRDRRIDAYAPITQRYLDQETR"
     gene            276..1370
                     /locus_tag="VHS97_18155"
     CDS             276..1370
                     /locus_tag="VHS97_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX3450184.1"
                     /translation="MDQLPALLDYRMPAEWEPHAATWIAWPHNRDDWPGKFEPVPWVY
                     TEIVRHLSRVEQVHIIVRSERMKRRVSDQLDCVGVDLEAVHFFEAPTDRVWLRDSGPT
                     FVAKNQSASDSGDPIGLVGWKFNGWAKYDNHHRDSRLPRMFERWLELTRWVPRVEQGG
                     KRVRVVMEGGAIDVNGRGSLLTTEECLLGEVQARNPGLDRERVEKIFADYLGVRHVIW
                     LGQGIYGDDTHGHVDDLARFVAPATVVTVVEPRAEDPNHEPLSENLKRLQAAYDQDGQ
                     PLRVVELPMPRPVVFDGQRLPASYANFYIANGLVLVPTFNDPADRIALDTLAVLFPDR
                     EVVGIHSVDLVLGLGTLHCLSQQQPAGSIL"
     gene            complement(1359..>2666)
                     /locus_tag="VHS97_18160"
     CDS             complement(1359..>2666)
                     /locus_tag="VHS97_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450185.1"
                     /translation="ITASSALVSTAAAQAPKAGGSSATGISKPSVGQGRVSQNQLSDE
                     ECRAYAEAVVNAIDSGNRAAFTAQIDWDSLLDTVMINMDLTDKQRQELKTGLKSGIDS
                     DTGLTGQLVKNSQQGGKVDFLRTRPNHGRQVILFRMIRPMHAGGVGYFEFVPERSAGG
                     KIRAVDLYVFSSGEFISTTLRHALLPVVANQSRSILSKLVTGERAYVKDFPKLSNVTA
                     LINEGKMQEALAVIKGMQPETQKLKIILLNRLRAAQSSDEKEYSAVLDDFRNLYPKDP
                     CLDLLLIDYYTLKKDFPKALETIDRLDKSIEGDPYLNIIRAGVSEMRGDLTEAARSAG
                     RAMEQEPTLAPAYFSAVGISMKAGKYDETLSLLKALDQKFHTQFNDLKTVPQYAGFVK
                     SPQYPQWLEYLAQKENPQQVKKSQSKPAVGKNRMRAPKAKSKN"
BASE COUNT          525 a          793 c          763 g          585 t
ORIGIN      
        1 aatcggatcg gccatgaagg tcccgctggg ggcggcctgg aattctgggg cggttcgttc
       61 gtggccgacc cattcggacg ggtaatcgcc aaagctggtc ccggtaccga ggaagtggtg
      121 atcgcccgat gcgaccccaa actccaggaa gaaactcgtc gcaactggcc attcttgcgc
      181 gaccgccgca ttgacgccta cgccccgatc acccagcgct acctcgacca agaaacacgc
      241 taacaactcg atgcccttca tgactaacga cttccatgga ccagctcccc gctttgcttg
      301 attatcggat gcctgccgaa tgggaacccc atgcggcgac ctggatcgcg tggccccata
      361 accgcgacga ttggcccggc aagtttgagc cggttccgtg ggtttacact gagatcgtcc
      421 gtcatctcag ccgcgtggaa caggtccata tcatcgttcg aagcgaacga atgaaacgcc
      481 gcgtgtctga tcagctcgat tgcgtgggcg tagacctcga agcggttcac tttttcgagg
      541 ctcccaccga tcgcgtctgg ctcagagatt ccgggcccac gttcgtcgcc aaaaaccagt
      601 ccgcatcgga ttccggcgat cccattggtc tggtgggatg gaagtttaac ggctgggcca
      661 agtacgacaa ccaccatcgc gacagccgcc ttcctcgcat gtttgaacga tggctcgagc
      721 taacgcgctg ggtgccgcgg gtcgagcagg gggggaagcg agtgcgggtc gtcatggagg
      781 ggggtgcgat cgacgtcaat gggcgcggtt cgctgttgac gaccgaggaa tgcctgctcg
      841 gcgaggtcca ggcgcgcaac cccggcctcg atcgcgaacg cgttgaaaag attttcgccg
      901 actacctggg agtccgccac gtcatctggc tgggccaggg tatctatggc gacgatacgc
      961 acgggcatgt cgacgatctg gcgcgctttg tcgcgccagc aacagtagtg acggtcgtcg
     1021 agccgcgcgc cgaagaccct aatcacgaac cgctaagtga gaacctcaag cgcctgcaag
     1081 cggcttacga ccaagatggt cagccgctgc gcgtcgtcga actgccgatg cctcgcccgg
     1141 tggtctttga tggccagcgg ctacccgcca gctatgccaa cttctatatc gccaacggtc
     1201 tggtactcgt accaacgttc aacgacccgg ctgaccgcat cgcgctcgat acgttggccg
     1261 ttttatttcc cgatcgcgag gtcgtcggca tccatagcgt cgacttggta ctgggactcg
     1321 gaacacttca ctgtctatcg caacagcagc ccgccggctc aattctttga cttggccttc
     1381 ggtgcgcgca tgcgattctt gcccactgct ggcttgctct gagatttctt cacctgctgg
     1441 ggattctcct tctgtgcaag gtattccagc cactggggat actgaggtga tttcacaaaa
     1501 cccgcgtact ggggtacggt tttcaggtcg ttgaattgcg tgtggaactt ctggtcgagt
     1561 gccttgagca gcgacagagt ctcgtcgtac tttcccgctt tcatcgaaat gcccaccgcc
     1621 gagaaatatg caggtgcaag cgtgggctct tgttccatgg cacgtcccgc cgaccgagca
     1681 gcttccgtca ggtcgcccct catttcgctg acgccggcgc ggattatgtt gagataggga
     1741 tctccttcaa tcgatttgtc cagccggtca atagtctcga gcgccttggg gaagtccttc
     1801 ttgagcgtgt agtaatcgat caacaggagg tcgagacaag gatctttggg atacagattc
     1861 cgaaagtcat caaggacggc ggaatactct ttctcgtcgc tggattgggc cgcccgaaga
     1921 cgattcagca ggataatttt cagcttttgg gtctccggct gcatcccctt gattacggcc
     1981 aaagcctcct gcatcttccc ttcgttgatc agggcggtga cattcgacaa cttgggaaaa
     2041 tctttgacgt aggctcgttc gccagtcact aatttgctga gaatgctccg cgactgattg
     2101 gcgacgaccg gtaagagcgc atgtcgcagg gtcgtcgaga tgaactcacc gcttgaaaaa
     2161 acgtacagat cgaccgcgcg aatctttccg ccggccgatc gctcggggac aaactcaaaa
     2221 taaccgactc cacccgcatg catcggccgg atcatgcgaa acaggatcac ttgccggccg
     2281 tggttcgggc gggtccgcag aaaatcgacc ttcccccctt gttgcgagtt cttgacaagt
     2341 tgccccgtga gacctgtatc gctatcgatc ccgctcttga gccccgtctt gagttcctgg
     2401 cgctgtttgt cggtgagatc catgttgatc atgactgtat ctagaagtga atcccaatcg
     2461 atctgcgctg tgaacgccgc tcggttaccc gaatcgattg cattgacgac cgcttcggca
     2521 taagcgcggc actcttcatc ggaaagctga ttctgcgaaa cccgaccttg gcccaccgac
     2581 ggctttgaga tccccgtcgc cgaggagccg ccggctttgg gagcttgagc cgcggccgtg
     2641 gaaaccagcg ccgacgaggc agttat
//
LOCUS       DATAQL010001055         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3771037, whole genome shotgun sequence.
ACCESSION   DATAQL010001055 DATAQL010000000
VERSION     DATAQL010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771037"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VHS97_18165"
     CDS             <1..193
                     /locus_tag="VHS97_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450186.1"
                     /translation="LVFENNVIRDTRSGSQQTQSVGILVEDRVGPVQIGSNQIEAGKP
                     IDDRRRSETQFGSQAQSTP"
     gene            268..582
                     /locus_tag="VHS97_18170"
     CDS             268..582
                     /locus_tag="VHS97_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3450187.1"
                     /translation="MTTPDIFAVLANPVRRRILELLMGRPCTVNHLVREFQLHRPAVS
                     EHLQVLRNARLVRDERRGRERYYHLDPARLIEVDEWIKPFARYWHERMQALDAILEEE
                     ER"
     gene            579..995
                     /locus_tag="VHS97_18175"
     CDS             579..995
                     /locus_tag="VHS97_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3450188.1"
                     /translation="MTERGVIRLSQFINHPPAKVWQALTDPTIHAKWWAAGDVKPVVG
                     HRFTLDMGSWGQQPCEVLAVETERLLSYSFAPGTLDSTITWRLEPENDGTRLHLEHSG
                     FDLDSPLGKKAFEGMGSGWPAVLKRIAPAIDSLVVA"
     gene            1207..2433
                     /locus_tag="VHS97_18180"
     CDS             1207..2433
                     /locus_tag="VHS97_18180"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX3450189.1"
                     /translation="MNAINRPARSCILTVNGGSSSLKFAVFDHQQAQAGSPERLIAGK
                     IERIGLPDARVTVSGPDGKVQETSQVSAPDLGASAEALITVLDKHVGCEALVGAGHRV
                     VHGGPRYHRPEPITAEVIAELRRLSPLDIDHLPGEIALIERFQRLRPGLFQVACFDTS
                     FHHDMPRVARIVPIPRRYEAAGVRRYGFHGLSYAYLIEELARVAGPEAALGRVILAHL
                     GSGASLAAVRQGKSVDTTMGLTPASGIVMGTRTGDLDPGLPFFLGECSGTTTDQFHAL
                     VNHESGLLGVSETSPDLRDLLAQEVRDVRAAEAVALFFYQARKAIGALAAALGGLDIL
                     VFSGGIGENSPEARARICEALGFLGITLDQDRNHANEPVISAGDARTLVRVIRTDEES
                     MIARGVASLLAAANSG"
     gene            2488..2736
                     /locus_tag="VHS97_18185"
     CDS             2488..2736
                     /locus_tag="VHS97_18185"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450190.1"
                     /translation="MDTPLDRDLQSKMDAYWRAANYLSVGQIYLYDNPLLAEPLKLEH
                     VKPRLLGHWGTTPVQNFIYVHLNRIIKQYDLNRIGFTW"
     gene            2781..2897
                     /locus_tag="VHS97_18190"
                     /pseudo
     CDS             2781..2897
                     /locus_tag="VHS97_18190"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2953..>3844)
                     /locus_tag="VHS97_18195"
     CDS             complement(2953..>3844)
                     /locus_tag="VHS97_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX3450191.1"
                     /translation="PQWADGATIDRFAALPGLERTEQKPQLNAGGSWTLPNGSVLVQT
                     LSLDRFADTDKTARRRIETRLLVRQQGEWTGYSYRWNAVQTDAELVPAAGSSAELEVA
                     DPSEPGGRREQTWRFPSRTECLVCHSRAAGFILAFTPLQLDRDHDYGGVIDNQLRTLE
                     HIGVFQGKLPERRADRPRLVNPYDARAPQEARVRSYLHVNCSICHVSEGGGNSNMELG
                     LATPLQKTRLVDEVPFHAHLGIADARLVASGSPERSVLYRQISVRGTDQMPPLGSTEV
                     DRDAVKLIADWIRGLPSLKR"
BASE COUNT          744 a         1160 c         1207 g          733 t
ORIGIN      
        1 tttggtcttc gagaacaacg tgatccgcga cacgcgatcg ggttcgcaac aaacccagtc
       61 ggttgggatc cttgtcgaag atcgggtcgg tccggtccag atcggatcga accagatcga
      121 ggcgggcaag cccatcgacg accgccggcg gagcgagacg caattcggat ctcaggcgca
      181 atccacgccg tgaggccatg aatttcgacg accatttcgc actcccgtac ttgacacgtc
      241 ggaaacttcc gacataatgt gctcagcatg acgacgccag atattttcgc cgttttggca
      301 aacccggttc gccgccggat tctggaactc ctgatgggtc ggccatgcac ggtcaaccat
      361 ctggttcgtg agttccagtt gcaccgccct gcggtttcgg agcatttgca ggtgctgcga
      421 aatgcaaggc tggttcgtga tgagcggcgc ggtcgcgagc gctactacca tctcgatccg
      481 gcgcggctga tcgaggtcga cgaatggata aagccatttg cgcgctactg gcacgagcgt
      541 atgcaggccc ttgacgccat tttggaagag gaggaacgat gacagaacga ggcgtgattc
      601 gattgtcgca gtttatcaat catccccctg cgaaggtttg gcaggcgcta accgatccga
      661 caatccacgc gaaatggtgg gcggcgggcg acgtgaaacc ggtggtcggg catcgtttca
      721 ctctggatat gggctcctgg gggcaacaac cgtgtgaggt gctcgcggtc gagactgagc
      781 gtctattgag ctatagcttc gcacccggca cgctcgattc gacgatcacg tggcggcttg
      841 agccggaaaa cgatggcacg cgtctgcacc tcgagcactc gggttttgac cttgactctc
      901 ccctgggaaa gaaagcattc gaaggcatgg gtagcggctg gccggcagtc ctgaaacgta
      961 tcgcgccggc catcgactcc ctcgtcgtcg cctaaggatg atcgaaccag cattcgcccg
     1021 gcgcggtcat gaggtcctat ttgatcggaa catcagcatt cgaatgcgag ccgctctggc
     1081 gggaggccag cgatgtccgg tcggttgtgc gctccaaccg ggtctactat actgccaggc
     1141 gtgaggtatc cttggccctc ggacactcgg ctgattggtc cttcaatcac caccggtgaa
     1201 gacgacatga acgcgatcaa tcggccggca cgatcgtgta ttctcacggt caacgggggc
     1261 tcgtccagcc tgaaattcgc cgtctttgat caccagcaag cgcaagccgg ctcgcctgag
     1321 cgtctgatcg ccggcaagat cgagcggatc ggtctcccgg atgcccgcgt gactgtctct
     1381 ggaccggacg gaaaggtaca ggagacgtcg caggtaagtg cccccgacct gggggcgtcc
     1441 gcggaggcgc tcatcaccgt tctggacaaa catgtcggtt gcgaggcgct cgtcggcgcg
     1501 gggcaccgtg tcgtacacgg aggaccgcgc taccaccgtc ctgagccgat cacggccgag
     1561 gtcatcgcgg aattgcgccg gctcagtcct ctcgatatcg atcatctgcc aggcgagatt
     1621 gcgctgatcg aacggttcca gcggctccgg cccgggttgt ttcaagtcgc ctgcttcgac
     1681 accagctttc atcatgacat gccccgcgtc gcccggatcg tgccgattcc gcgccgatat
     1741 gaggccgccg gggtccgccg ctacgggttt catggcctct cgtacgcgta tctcatcgag
     1801 gagctggcac gggtggccgg accggaggcc gctctaggac gcgtcatcct ggcccacctc
     1861 ggctcggggg ccagcctggc cgcggtccgt caggggaagt cggtcgatac caccatgggc
     1921 ctgacgccgg cctcgggaat cgtgatggga acgcgcaccg gagacctcga tcccgggctc
     1981 ccctttttcc tgggcgaatg ctccggcacg acgaccgacc agtttcacgc gctcgtgaac
     2041 cacgaatcgg gcctcctggg cgtctccgag acaagccccg atttgcgtga cctgctagcg
     2101 caagaagtgc gtgacgtccg agcggccgaa gcggtcgcac tattctttta ccaggcccgg
     2161 aaagcgatcg gggccttggc ggcggcactc ggtggactcg acatcctcgt attctccggc
     2221 gggatcggcg aaaattcgcc ggaggcccga gctcggatct gcgaggcact cggattcctc
     2281 ggcatcactc tggaccaaga tcgcaaccac gcgaacgagc ccgtgatcag cgcgggcgac
     2341 gcccgcaccc ttgtgcgagt tattcgcacc gatgaagaat cgatgatcgc gcggggcgtg
     2401 gccagcttgc tggcggcggc caactcaggt tgaacgagcg cccgagccag cagccggcat
     2461 ctcaaacttg cagcaaggaa gacgactatg gatacgccgc tcgaccgaga tttacagagc
     2521 aagatggacg catactggcg tgccgccaac tatctgtcgg tcggccagat ttatctctat
     2581 gacaacccgc tcttagccga gccgctcaag ctcgagcacg tcaagcccag gctgctgggg
     2641 cactggggta cgacgccagt ccaaaacttc atctacgttc acttgaatcg tatcatcaag
     2701 cagtacgacc tgaatcggat cggtttcacc tggtaggcga cgtggttgac cgcgtgtcgg
     2761 agtagacaaa gacggccgcc tatttgaagc aggcggttcg cgacaagctg atcgatcacc
     2821 ggcaatacat caccacttac ggtgaagata tgcccgagat ccgcaactgg acgtggaacg
     2881 gaacgacagc ccggtgagca actgagttaa ggtttgggtg aatggcttca cgttacgccc
     2941 atgttgactt tcttatcgtt tgagcgaggg cagtccgcga atccagtccg caatgagctt
     3001 gaccgcgtcg cggtcgacct cggtggatcc caaaggtggc atctggtcag tcccacgtac
     3061 agagatctgc cgatagagaa cagatcgttc gggtgagccg gaagcgacga gacgagcgtc
     3121 ggcgatgccc aggtgggcgt ggaagggaac ctcgtcgacg aggcgcgtct tttgaagcgg
     3181 cgtggccagc cccaactcca tgttggagtt accgcctccc tccgagacgt gacagattga
     3241 gcaattgaca tgcaaatacg atcgcacccg agcctcttgc ggagctcgcg catcgtacgg
     3301 attcaccagg cggggccgat ccgcgcgccg ttcgggcaac ttgccctgga acacaccgat
     3361 gtgctcgagc gtgcgaagct ggttgtcgat gacgccgccg taatcgtggt cgcggtcgag
     3421 ttgcaggggt gtaaaggcca gaatgaaacc agccgcgcgc gagtggcaga ccaggcactc
     3481 ggtgcgggag gggaaccgcc aggtctgctc acgccgaccg cctggctcgc tcggatcggc
     3541 cacctccaac tcggcggagc tgccagcggc cgggaccaat tcggcgtcgg tctgtactgc
     3601 gttccagcgg taggagtagc cagtccattc cccctgttga cggaccaaga ggcgcgtctc
     3661 aattcgccgg cgcgccgtct tgtccgtgtc ggcgaatcgg tcgagactga gcgtttgtac
     3721 caaaaccgag ccgttgggca gtgtccagga tccgcccgcg ttgagctgag gtttctgttc
     3781 ggtgcgctcc agtccgggca gagcggcgaa tcgatcgatc gtggctccat cggcccactg
     3841 cgga
//
LOCUS       DATAQL010001056         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3313819, whole genome shotgun sequence.
ACCESSION   DATAQL010001056 DATAQL010000000
VERSION     DATAQL010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313819"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VHS97_18200"
     CDS             <1..1035
                     /locus_tag="VHS97_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450192.1"
                     /translation="AIEPKRALAQTRTKLADLLVRNGRNQGEAEPVYREALQAQEVLA
                     ASRSATVLDRFHLGQTLKSQGDSLRLCGQFAQAKPVYARALSELQLGLKRPQAETTLK
                     DAIGALEKLAARESPSRYDRRILASLQGNLGELMIQLARYSEAGPYLASSKAGLDSLV
                     ALEPLSSVSQSCLGIVMEAEARLLRQTGKPEQAKIALDGAIAHQRRALGLSHNDPSYR
                     DQLGSHLLELARVDLELGLYDKARAIAADLPTAVPVSKRPQGCVDAARIMARLIDKVG
                     ADATRQQSERERVTRSYCGRLALFLREALDADPKLAEPIKSDADIKGIYSHPEFREII
                     DSLVHLSSIH"
     gene            1035..2756
                     /locus_tag="VHS97_18205"
     CDS             1035..2756
                     /locus_tag="VHS97_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450193.1"
                     /translation="MTQRRTCAGDTLSIWLTAIFSLAAAIAMGFGLYLAFGREDTEVL
                     ESPLMYSVARQLLDGPWVLYGPFGARNPLVLIHAPLYYHLAALLAWPIFRAGVDPIWA
                     ALAAGRAISFVGLGLTGAAAYRLARIDGARPRAGLWAALLIAASPAIDVMPYAVRPDM
                     LGIALQTTGVLLVLATRQVPRSAGALLPAAFAAFGLAACIKQHFVTAPAISALLLLSA
                     SATGRLPFKFVARGLATGLAIVLLVYGIEEVATGGRMSQAAIQAAATTSRVHPGSWTR
                     FLIVAFTILGRNSGLLALLVAAGVAGIRSRQTIGRRFLEVTGLVVIVTIVLRSAPISL
                     EGDVDLWAEFVNTFTITVAVLFVLPACYLLSRRTLATGSVDNALLYYLAAECLGVMIL
                     SRASTGAWVNYGIQAVIFASILTARALDRACDFVPSTRALLPFVLAALVFLCNVISGA
                     RTTASARFYDRLALARIFEYYRHPTTEFFFVSRPGDNRVYGQRALVYDDWLYPVFESI
                     HFAEPQSVWLTGALTADGINYVVTTSDSTAIEGLGRSFHDLGYIRGIKVGKFFVWERT
                     GLLRSSR"
     gene            2954..>3562
                     /locus_tag="VHS97_18210"
     CDS             2954..>3562
                     /locus_tag="VHS97_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX3450194.1"
                     /translation="MIRLPQRGWRLTCGSQFAWTLALGITASIIASVPGPAAWAEGAD
                     PTVSKSVEFNRDIRPILADKCFKCHGPDSRERKGKLRLDNDKDARAPAASGSAAVVPG
                     KPEESELYQRIIAQDADERMPPPKSGKSLSAADVATLKTWIEQGAEYQGHWAFLPPRR
                     PPLPPVKNSGWCSNPIDFFILAKLEAEGLKPAAEADKVTLIRR"
BASE COUNT          630 a         1119 c         1109 g          704 t
ORIGIN      
        1 gcgatcgaac cgaaacgtgc gctggcacaa acgcgcacga agctggccga tctcctggtt
       61 cgaaacggtc gcaaccaggg ggaagccgag ccggtttatc gcgaggcact ccaagctcag
      121 gaagtccttg ccgcctcacg gtccgcgacc gttcttgatc gctttcacct cggacaaact
      181 ctcaagagcc agggagactc actcaggttg tgcgggcaat ttgctcaagc aaagcccgtc
      241 tacgcgcgcg ccctgagcga gctccagctc ggtctgaaac ggccacaagc cgagaccacc
      301 ctcaaggacg cgatcggtgc actcgagaaa ctggcggcgc gtgagtcacc gagccgctac
      361 gatcggcgaa tcctggccag ccttcagggc aacctgggcg agttgatgat ccagctcgcg
      421 cggtatagcg aggcgggtcc atacctcgcc agttccaagg ccggcttaga ttcgctcgtc
      481 gccctggagc ccttgtcaag cgtttcccag agctgtctgg gcatcgtcat ggaagcggaa
      541 gcgaggctgt tgcggcagac gggcaagcca gagcaggcga agattgctct ggacggcgcg
      601 atcgcacacc agcgccgtgc gctcgggctc agtcacaacg atccctccta tcgcgaccag
      661 ctcggcagcc acttgctgga actggctcgc gtcgacctcg agctcggcct ctatgacaag
      721 gccagagcga ttgcagcgga tttgcccacc gcggttccgg tctccaagcg gcctcaggga
      781 tgcgtcgacg ccgcgcggat catggcgaga ctgatcgaca aggtcggcgc cgatgccacg
      841 cggcaacaaa gcgagcgaga gcgagtcact cgtagctatt gcggacggct cgccttattc
      901 ctgcgcgagg cgctcgacgc cgaccccaag ctggccgagc cgatcaagtc tgacgccgac
      961 atcaaaggca tttattcgca ccccgagttt cgtgagatca tcgactcgct tgtgcacttg
     1021 agcagcatac actgatgacg caacgacgca cgtgcgccgg cgatacgtta tcgatctggc
     1081 taacggcgat cttctcgctg gcagcggcca tcgccatggg cttcgggttg tacctggctt
     1141 tcggccggga agataccgag gttctcgagt cgccgctcat gtactcggtt gcccggcaac
     1201 tcctggacgg accgtgggtg ctttacggcc cgttcggcgc ccgcaacccc ttggtgctga
     1261 ttcatgcgcc cttgtactac catctcgccg ccctcttggc atggccgatc ttccgagcag
     1321 gggtcgaccc gatatgggcg gctcttgcgg ccggacgggc gatctcgttt gtggggttgg
     1381 gcttgaccgg ggctgcggcg taccggctgg ctcggatcga tggagcgcgc ccccgggccg
     1441 gattgtgggc cgctctcctg attgcggcgt cgccggccat cgacgtgatg ccctatgccg
     1501 tccgccccga tatgctgggc atcgcgctcc agacgacggg cgttcttctc gttctcgcga
     1561 cacggcaggt cccgcgatcg gcaggtgccc tcttgccggc ggcgtttgcg gcattcgggc
     1621 tggccgcttg catcaagcag cacttcgtaa ccgcgcccgc gatcagtgcg ttgctcttac
     1681 tgtcggcctc cgcgacgggc cggctcccgt tcaaattcgt cgcgcgcggc ctggctaccg
     1741 gcttggcgat tgtgctcttg gtctacggga tcgaagaagt ggccacagga ggcaggatgt
     1801 cgcaagcggc cattcaagcc gccgccacca cgagccgggt ccaccccggg agctggactc
     1861 gttttctcat tgtagctttc acgatcttgg ggagaaacag tgggctgctg gcgctcttgg
     1921 tggctgccgg tgtggccggc ataagatcac gccagacgat cggccggcgc tttctcgagg
     1981 taacaggact cgttgtgatc gtcacgatcg tcttgcgaag cgcaccgata agtcttgaag
     2041 gcgacgtgga cctctgggcc gagtttgtta acaccttcac tatcacggtc gcggtcttat
     2101 tcgtcctccc ggcgtgctat ttgttatcgc gaaggactct ggcgacaggc agcgtcgata
     2161 atgctctcct ctactacctg gcggcggagt gtctgggggt gatgattctg agtcgcgcca
     2221 gcaccggcgc atgggtaaac tatggcatcc aggccgttat tttcgccagc attctcaccg
     2281 cgcgggcgct tgatcgggcg tgcgacttcg tcccttcgac gcgcgctctc ttgccgttcg
     2341 tgctggccgc gctggtcttt ctgtgcaatg tgatcagtgg tgccaggacg acggcaagcg
     2401 cgcgtttcta cgatcggctc gctctggcgc gaattttcga atattaccga cacccaacga
     2461 ccgagttctt cttcgtgagc agacccggcg acaaccgcgt gtacgggcag cgtgccttgg
     2521 tttatgacga ctggctctat cccgttttcg agtcaatcca tttcgccgaa ccgcagtccg
     2581 tctggctaac cggggctctc accgccgacg gcatcaacta cgtcgtcacc acgtctgata
     2641 gcactgcaat cgaaggcctg ggccgttcat ttcacgatct gggctacatc cggggcatca
     2701 aggtggggaa attcttcgtc tgggagcgca ccggattgct gcgctcgtcc cgttaatggc
     2761 cggccgttcc agcgcggcgg tccaggttcg ggtgccgatc cgcgcgatac gaattggtgg
     2821 agaatgtcca ggccaccgag taaagtaaag ggtgttcggc cagacctggc tggatcgatc
     2881 ccaccctttt tcgccaaact gcttcaccgg ccagatagat ggcggctgac acgaaccaaa
     2941 agggcaacga agcatgatcc ggcttccgca acgcggctgg cgtctgacct gcgggtcgca
     3001 attcgcttgg acgctggccc tcggcatcac cgcctcgata atcgcctccg tgccgggacc
     3061 tgccgcttgg gcggaaggcg cggacccgac cgtatcgaag tcggttgagt tcaatcgcga
     3121 catccggccg atcctggccg ataagtgctt caagtgccac ggtcccgact ccagggagcg
     3181 gaaaggaaag ctgaggctgg acaatgataa agatgctcga gccccggccg cgtcggggag
     3241 cgcggccgtt gtgcccggca agccagagga gagcgagctc taccagagaa tcattgcaca
     3301 ggacgctgac gagcggatgc cgccgccaaa gagcggtaaa tccctttcgg ccgccgatgt
     3361 cgccaccctc aagacctgga tcgagcaggg tgcggagtat caagggcact gggcgttcct
     3421 tcccccccgt cgtccgccat tacctccggt caagaactcg ggctggtgct ccaatccgat
     3481 cgacttcttt attctggcca agctggaggc cgagggattg aagcccgcgg cggaggccga
     3541 caaggtcaca ctgatcaggc gg
//
LOCUS       DATAQL010001057         4773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5449477, whole genome shotgun sequence.
ACCESSION   DATAQL010001057 DATAQL010000000
VERSION     DATAQL010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5449477"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VHS97_18215"
     CDS             <1..1084
                     /locus_tag="VHS97_18215"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3450195.1"
                     /translation="FGISFTRRALNQASALVNVYLDGTIQVSTGGTEMGQGLNTKIRQ
                     IVAAEFALPLESVRVMPASTEKNNNTSPTAASASTDLNGTAAARACEIIKGRLAEAAA
                     RHFASAAEGIEASATHIRFERAAVHDIRRPGAWLKFRELVKLAYEERVDLGARGFYAT
                     PGVDFNRETGRGNPFLYFTNGAAVSEVVIDRLTGELAVTRVDILMDLGRSLNPDIDRG
                     QVIGGFVQGMGWVTTEELLYSETGDLLSHSPNNYKIPTADCLPRVLLVDFLNNSGNPI
                     NLLGSKAVGEPPFVLGISVAVAAKSALSSLSPGRSPPLSFPVTSEELLKHISALGQDE
                     DPMKLANAMLSRVHEREDQIRAGNQV"
     gene            1065..1547
                     /locus_tag="VHS97_18220"
     CDS             1065..1547
                     /locus_tag="VHS97_18220"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX3450196.1"
                     /translation="MPATKSESELMSIAIAKARDGIAAGQSPFGAIIVRDGVVVASTH
                     NTVWRDTDPTAHAEVNCVRAAASALKLIDLSGCEMYSTCEPCPMCLSAIHWSKIDRVV
                     FGATIADAALAGFNELQVDAKLLAEMGKSTLKVESGLLREECALLFDLWKKTGLCGTY
                     "
     gene            1600..3375
                     /gene="ade"
                     /locus_tag="VHS97_18225"
     CDS             1600..3375
                     /gene="ade"
                     /locus_tag="VHS97_18225"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEX3450197.1"
                     /translation="MSNSQDDTQGRTVPMLHVARAAAQRRIRVALDHEPGDLLLTGGQ
                     VVNVFNRRVEPADVVIADGRIAGVGCYSWRAAQTISVSGQVILPGLIDAHMHLESTLL
                     TPAELARLIVPMGTTTVISDSHEVANVMGVRGIDMLAAACEGLPLDLFFMASSCVPAT
                     AWEDAGAALGPAEVSTLLSRRHVLGLAEVMDIPAVLQGGQEVFEKLETALAAGRVIDG
                     HAAGLSGRELIAYAGAGIRSDHESTTAEEARAKCALGMLVQVREGSSAQNLDALLPLL
                     AAGELEDSWCLVTDDIFPNDLKCHGHIDGLLRRVVAGGVSPAAAVRHASFVPARHYGL
                     SDRGAVAPGYRADLVMVEDVAQFRVRMVLKGGKVVARDGKCVPDAPAPRLQPENTVHL
                     RPLDEQAFRLPLAAEMCPVIEIVPDQIITRHTRRTVRRLGGQWAFDPERDVLLIASIE
                     RHRASGRIGLGLVSGFGLTRDGALGSSVAHDSHNLVIAGTNPRDIMVCARALADHGGG
                     FVAAAQGQVLAILPLPVAGLLSLDDADQVCRQLDWVNRAAHSLGCPLDAPFGTLSFLA
                     LPVIPELRITTRGVFDVREQRFLTL"
     gene            3400..>4773
                     /locus_tag="VHS97_18230"
     CDS             3400..>4773
                     /locus_tag="VHS97_18230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX3450198.1"
                     /translation="MEASSHRAVLEPRTNLPEPAVLADSFHVNPDLAPTSPAQRRWGT
                     KDIAALWISMSACIPTYMIASSLIAEGMNWWQAVLTIFLGNTIVLVPMILNAHAGTKY
                     GIPFPIYCRASFGILGANVPALLRALVACGWFGIQTWIGGGAIYQIVAIYIPAWKELA
                     LIPGLGINGAQLVCFLAFWAVNMFVIWMGIDSIRILLNIKAPLLIVLGLALLAWAYRE
                     AHGFGPMLAQPSQFDPGQPKAGQFWTFFFPALTAMIGFWATLSLNIPDFTRYARSQRD
                     QILGQALGLPTTMALYSFIGVAVTSATVTIYGNAIWDPVVVLSKFRDPLVLSIGMVSL
                     CLATLATNIAANVVGPANDFSHVWPRRISFRTGGLITGVVGILIQPWKLIADPTGYIF
                     RWLVAYSSLLGAVGGILIADYFVLRRTRLDLGGLYNSRGPYWFVHGFNPLAMIALVIG
                     IAPCVPGF"
BASE COUNT          788 a         1474 c         1522 g          989 t
ORIGIN      
        1 gttcgggatc tcgttcacaa ggcgcgcgct caatcaggcg agtgcacttg tgaacgtata
       61 cctggatggc acgatccaag tctcgaccgg cgggaccgag atggggcagg ggctaaacac
      121 caaaattcgc cagatcgtgg ccgcggagtt tgccctgcca ctcgagtcgg tccgggtcat
      181 gccggcctcg accgaaaaga acaacaacac ctcgcctacg gctgcgtcgg cgagcacgga
      241 tctgaacggg acggccgcgg cgcgcgcttg tgagatcata aagggccggc tcgccgaggc
      301 ggccgcgcgc catttcgcat cggcggcgga ggggatcgag gcttctgcga cccacatccg
      361 cttcgagcgc gcggcggtgc acgacatccg ccggcccggc gcctggctca agtttcgcga
      421 gctcgtcaag ctggcctatg aagagcgcgt cgacctgggc gctcggggct tttacgccac
      481 gccgggggtc gacttcaatc gcgagacggg ccggggcaat ccgttccttt acttcaccaa
      541 cggtgccgcg gtctccgagg tcgtgatcga ccgactgacc ggcgagctcg ctgtgacacg
      601 ggtcgacatc ctgatggatc tgggtcgatc gctcaacccc gacattgatc gcggccaggt
      661 gatcggcggg ttcgtccagg gaatgggctg ggttaccacc gaggagctgc tctattccga
      721 aaccggtgac ttgctatcgc actcacccaa caattacaag attccgaccg ccgactgcct
      781 gccgcgcgtc ctcttggtcg acttcctgaa caattccggc aatccgatca acctgctggg
      841 cagcaaggcc gttggtgagc cgccgttcgt tctcggaatc agtgtggcgg tggcagccaa
      901 gtctgccctg tccagtctgt cgccgggccg ttcaccgccc ctgtcgttcc cggtgaccag
      961 tgaagagctg ttgaagcata tctctgcgct cggccaagat gaagatccga tgaagttggc
     1021 caacgcaatg ctatcgagag tccacgagcg agaggaccaa atccgtgccg gcaaccaagt
     1081 ctgaatcaga actgatgtcg atcgccatcg ccaaggcccg cgacgggatc gccgccggcc
     1141 aatctccatt tggagcgatc atcgttcgag atggcgtcgt ggtggcgtcg acgcacaaca
     1201 cggtatggcg cgacaccgac ccaaccgcac acgccgaggt gaattgcgtc cgggccgcgg
     1261 cgtcggcgct caagctgatc gacctctccg gttgcgaaat gtattccact tgcgaacctt
     1321 gtccgatgtg tctctccgcg atccactggt ccaagatcga tcgcgttgtt ttcggagcga
     1381 cgatcgccga cgccgccctg gccggcttca acgagttgca agtcgacgcc aagctcctcg
     1441 ccgagatggg caagagcacg ctcaaggttg aaagcggcct gctacgcgaa gagtgcgccc
     1501 ttttgtttga tctttggaaa aagaccggct tgtgcgggac ctattgaaca cgatgtggcc
     1561 ggctcagtgc catgacgttg aggaggacag tgggtcgcca tgagtaatag tcaggacgac
     1621 acccaaggtc gaacggtccc gatgctccac gtcgctcgag cggccgccca gcgcaggatc
     1681 cgcgtcgcac tcgaccacga gcccggcgac ctcctgctaa cgggcgggca ggtcgtcaac
     1741 gtcttcaatc gccgtgtcga gccggctgat gtggtgatcg ccgacggccg catcgccggt
     1801 gtgggctgtt actcatggcg ggcggcccag accatctccg tttcgggcca ggtgatcttg
     1861 ccgggcctga tcgatgcgca catgcatctg gaaagcacgc ttttgacgcc ggccgagctg
     1921 gcgcggttga tcgtgccgat gggaaccacg acggtgattt ccgactcgca cgaggtggcc
     1981 aatgtgatgg gtgtgcgagg gatcgacatg ctggcggccg cgtgtgaggg gctaccgctg
     2041 gaccttttct tcatggcgtc gtcgtgcgtg cccgccactg cgtgggaaga cgccggggcc
     2101 gctcttggcc ccgccgaggt cagcacgctc ttgtcgcgcc gacacgtgct gggtctggct
     2161 gaagtgatgg atatcccggc ggtattgcag ggcgggcaag aagtcttcga gaaattggag
     2221 acggccctgg ctgccggccg cgtaatcgat gggcatgccg ccggcctgtc cggccgggag
     2281 ctgatcgcct acgcgggtgc tggcatccgc tccgaccacg aatcgacaac cgcggaagag
     2341 gcacgggcta aatgcgcgct gggcatgcta gtccaggtcc gcgagggctc gagcgctcag
     2401 aacctggacg cactgcttcc gctcctggcg gcaggagagc tcgaagactc gtggtgcctt
     2461 gtgacggacg atatcttccc taacgacttg aagtgtcacg gccacatcga cggcctcttg
     2521 cgtcgagtcg tggccggcgg tgtgtcgccg gccgcggcgg tccggcacgc gtcatttgtg
     2581 ccggcccggc attacgggct ttccgatcgc ggcgctgtcg cgccgggcta tcgcgccgat
     2641 ctggtcatgg tcgaagacgt cgcccagttc cgcgttcgta tggtgctcaa aggggggaag
     2701 gtcgtagcgc gcgatggcaa gtgcgtcccc gacgcacccg cgccgcggct ccagcctgag
     2761 aatacggtcc acttgcgacc gctcgacgaa caagctttcc gtctgcctct cgcagcggag
     2821 atgtgtcccg tgatcgagat cgtgcccgac caaatcatca cgcggcacac ccgccgcacc
     2881 gtccgccggt tgggcggcca gtgggcgttt gatccggaac gcgacgttct cttgatcgcc
     2941 agcatcgagc ggcaccgggc ctcggggcgg ataggcctgg gcctggtcag cggtttcggg
     3001 ctcacgcgcg atggagcgct gggctcgtcg gtcgcgcacg actcgcacaa cctggtcatc
     3061 gccgggacca acccacgtga catcatggtg tgcgctcggg ccctggccga tcacgggggt
     3121 ggttttgttg ccgccgccca gggccaggtt ctggcgatct tgcccttgcc tgtcgccggt
     3181 ttgctctcac tcgacgacgc cgaccaagtc tgccgccagc tcgactgggt caatcgcgcg
     3241 gcccactcgc tgggctgtcc tctggatgcc ccattcggca cgctctcgtt cctggccctg
     3301 ccggtgattc cggaattacg aatcaccact cggggggtct ttgacgtccg cgaacaacgg
     3361 tttctcacgc tgtgatccat ggtctggagg ttaatgccga tggaggcgag ctctcaccga
     3421 gcggtcctgg agccgagaac aaacttgccc gagccggctg tgctcgccga ctcatttcat
     3481 gtgaatcctg acctcgcacc gacgtcacca gcccagcggc ggtggggtac gaaagacatc
     3541 gcggctttgt ggatctcgat gtcggcctgt attccgactt acatgattgc gtcatccctg
     3601 atcgccgaag ggatgaactg gtggcaggcg gtgttgacca tctttctggg caacacgatc
     3661 gtgctggtgc cgatgatctt gaatgcgcac gcagggacca agtacggcat cccgtttccg
     3721 atctattgtc gcgcgtcgtt cgggatcctg ggcgcgaatg tgcccgcgct tttgcgagcc
     3781 ctggtcgcat gcggctggtt tggcatccag acgtggattg gtggcggagc gatttatcaa
     3841 attgtcgcta tttacatccc ggcgtggaag gagctcgcac tgattccagg cctgggcatc
     3901 aatggagccc aactggtgtg ctttctggcg ttctgggctg tcaacatgtt tgtgatctgg
     3961 atgggtatcg actcgatccg gatcctcttg aacatcaagg ccccgctcct gatcgtgctg
     4021 gggctcgcgc ttttggcgtg ggcgtaccgc gaggcgcatg gcttcgggcc gatgttggcg
     4081 cagccctcgc agtttgatcc ggggcaaccc aaagccggtc agttctggac gttctttttt
     4141 ccggcgctca ccgcgatgat cggcttctgg gccacgctct cgctcaatat tcccgacttt
     4201 acgcgttacg cccggtcgca gcgcgaccag attttgggcc aggcactcgg tctccccacg
     4261 acgatggccc tttattcgtt catcggtgtg gccgtcacat cggcgacggt gacgatctac
     4321 ggcaacgcga tctgggaccc ggttgtggtg ctttccaaat tccgcgatcc gctcgtgctt
     4381 tcgatcggga tggtatcgct ctgtctggca actctggcaa cgaacatcgc cgccaacgtg
     4441 gttggcccgg ccaatgactt ttcgcacgtg tggccgcgcc gcatttcgtt tcggaccggt
     4501 ggattgatca ccggtgtggt ggggatcctg attcagccgt ggaaactgat cgccgatccc
     4561 accggttaca tctttcgatg gctcgtcgct tattcttctc tattgggggc ggttggcggt
     4621 attttgatcg ccgactactt cgtcttgcgg cgcacgcggc tcgatctggg cggactgtac
     4681 aatagtcgcg ggccatactg gtttgttcat gggttcaatc ctctcgcgat gatcgcgctc
     4741 gtgatcggca tcgccccgtg cgtccccgga ttt
//
LOCUS       DATAQL010001058         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4378989, whole genome shotgun sequence.
ACCESSION   DATAQL010001058 DATAQL010000000
VERSION     DATAQL010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378989"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2144)
                     /locus_tag="VHS97_18235"
     CDS             complement(<1..2144)
                     /locus_tag="VHS97_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX3450199.1"
                     /translation="MKVRGLLLAVLLVPSLALADESNVVPLWPHGAPGQDGNKTPEKV
                     ADSSQGKARHRRVSSIHEPSLTVFLPPKDKATGAAVVILPGGGHRYLAIDHEGYDVAK
                     WLAGQGVAGLVLKYRLANEEGSNYKVDVHALADARRAVRLARSQAREWAIDPARVGVM
                     GFSAGGELAILAGTKFEEGKSDASDAIDRLNSRPDFLMLIYPGMRQAVLNVTKDTPPS
                     FLAVADDDRRCAEVCVAFYQALKKAGVPGELHIYASGGHGFGMTERPLPITTWTARLH
                     DWMADRGYLAKAGKPAAAAGPPARGRPSPKTEASPAVRIKAPKGFQVDLIYSVPQQTQ
                     GSWVNMTVDPKGRLIVSDQYGKLYRVSLPPIGGAVSAINVEAIDAPIGEAQGLLWAFD
                     SLYVVVNRGRKYDSGLYRVQDTDGDDRLDKVELLRKLNGGGEHGPHAVIMAPDGKSLY
                     IVAGNATALTAIDDSLVPRVWGEDNLLPRMVDGAGFMTSEKAPGGHIYRVSPDGKRWE
                     LVAIGFRNPFDIAFNRDGELFTYDSDMEWDVNMPWYRPTRVLNVASGGDFGYRNGSGK
                     WPPYYIDSLPPVVNVGPGSPTGIVFGYGSKFPVKYQEALYICDWSYGKLYALHMTPKG
                     SSYTGEVEEFLAGTPLALTDVVVNAKDGAMYFAVGGRNTQSGLYRVTYTSADAASVAG
                     VNSPATALMARELRHKLESLHGHKDPRAVETA"
     gene            2477..>3099
                     /locus_tag="VHS97_18240"
     CDS             2477..>3099
                     /locus_tag="VHS97_18240"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX3450200.1"
                     /translation="MAPSPMRSSWLLIWLFAVLGATERAALADDDFAFFESKIRPVLV
                     ERCHKCHSSQAPKPKGGLRVDSRTALRAGGSSGPAVVPGDLDASLLYQAITAAEGAVP
                     MPPTGRLSPAEIADFRRWITIGAPDPREKPATATSSPARVAAAQSHNWWSLRPVEQPS
                     VPSSAKATADWPRNPIDAFILAKLTEKGFTPAPEADRRTLIRRLSFD"
BASE COUNT          553 a         1007 c          962 g          577 t
ORIGIN      
        1 cacgcagtct cgaccgcgcg cggatctttg tggccgtgca gcgactcgag cttatggcgc
       61 agttcgcgcg ccatcagggc agtggcaggt gaattgacgc ccgcgaccga cgccgcgtcg
      121 gcgctcgtat aggttactcg atagagcccg gactgagtgt tccgtcctcc caccgcgaaa
      181 tacatcgcac cgtccttggc attgacgacg acgtccgtca aggccagcgg cgtgcccgcc
      241 agaaactcct ccacttcacc cgtgtaggac gagcccttcg gggtcatatg gagggcatag
      301 agcttgccgt agctccaatc gcagatgtag agggcttcct gatacttcac aggaaacttg
      361 gacccgtaac cgaagacgat gccggtggga gacccaggcc caacgttgac taccggcggc
      421 aggctgtcga tgtagtaagg cggccatttg cccgagccgt tgcggtagcc gaaatctccg
      481 ccactggcga cattgagcac gcgcgtgggg cggtaccacg gcatgttgac atcccattcc
      541 atgtcggaat catacgtgaa caattctccg tcccgattga aggcaatatc gaacgggttg
      601 cggaagccga tcgccaccag ctcccagcgc ttgccgtcgg ggctaacgcg gtagatgtgg
      661 ccgcccggtg ctttttcact ggtcatgaag ccggcgccgt cgaccatgcg cgggagcaga
      721 ttgtcctcgc cccagacccg cgggaccagc gagtcatcga tcgccgtcag cgccgtggcg
      781 ttaccggcga cgatatagag cgacttaccg tcgggcgcca tgatcaccgc gtgcggcccg
      841 tgctcgccgc cgccgttgag cttacgtagg agctcgacct tgtcgagccg gtcgtcgcca
      901 tcggtatcct ggacgcggta aagcccgctg tcatatttgc ggccgcgatt gacgaccacg
      961 tacagactgt cgaatgccca cagcaacccc tgcgcctcgc cgatcggggc gtcgatggct
     1021 tcgacgttga tcgccgagac tgccccgccg atcggcggaa gcgagactct gtagagcttg
     1081 ccgtactggt ccgagacgat cagccggccc ttggggtcga cggtcatgtt gacccacgag
     1141 ccctgcgttt gttgcggcac cgagtagatc aagtcgacct gaaagccctt gggggccttg
     1201 attcggacgg ccggtgacgc ctcggtcttg ggtgaaggcc gccctcgtgc cggcggaccg
     1261 gccgccgcag ccggcttgcc tgctttcgcg agataaccgc gatcggccat ccagtcgtgt
     1321 agcctggccg tccaggtcgt gatcggcagg ggacgctcgg tcatgccaaa gccgtggccg
     1381 ccgcttgcat agatgtggag ctcgcctggt acgcccgctt tcttgagtgc ctggtaaaac
     1441 gcaacacaga cctccgcgca acggcgatcg tcatcggcta ccgccagaaa ggagggagga
     1501 gtgtccttgg tcacgttgag cacggcctgg cgcataccag gatagatgag catcagaaaa
     1561 tcggggcgcg agttcaggcg gtcgatcgcg tcgctggcgt ccgactttcc ttcctcaaac
     1621 ttcgtcccag ccagaattgc cagctcgccg ccggcggaaa agcccatcac gcccacgcgc
     1681 gccgggtcga tcgcccactc ccgtgcctgg ctgcgtgcca ggcggacggc ccggcgggcg
     1741 tcggcaagcg catgaacgtc gaccttgtag ttcgagcctt cctcgttcgc aagccggtat
     1801 ttgaggacca gccccgccac cccctgcccc gcgagccatt tggcgacatc atagccctcg
     1861 tggtcgatcg cgagataacg gtggcctccc ccggggagga tcacaaccgc cgcaccggtc
     1921 gccttgtcct tgggagggag aaacaccgtc aaacttggct cgtggatgct cgaaacgcgg
     1981 cgatggcgcg ccttgccctg gctcgaatcg gcgacctttt ccggcgtctt attaccgtct
     2041 tggcccgggg ctccgtgcgg ccagagcggc accacgttgg actcatcggc caatgccaag
     2101 ctcggcacca gcaacacggc caggagcaac cctcgaacct tcatggcgtc tctctccaac
     2161 gactaagggg gggacaaagc gggctggatc ggcaagtcac gcacaagatt cgatgccggt
     2221 gccatcgcgg atggaagagt ggctttgcgt tcgcataact cgattaaccg tcggggccac
     2281 gtctctcatt ttgggaggat aaccgcacgg atgcaacaga ttcgcagtac gcagtcgccc
     2341 cgactcatcg acccagagac cgccacgtct tggtttgctg atgaccggcg ggcgccggcc
     2401 tgttacattc ttgttgaaca cctgactcgt ggaagtggat caacggcaat tgattattgc
     2461 gagggcgatc cagcgaatgg ccccttctcc catgcgttcg agttggctct tgatctggtt
     2521 gttcgccgtt ctcggggcga ctgagcgggc ggcgctcgcc gacgacgact tcgccttttt
     2581 cgagtccaag atccggccag tactggtgga gcggtgccat aaatgccact cgagtcaggc
     2641 cccaaagccg aagggtggcc tccgtgtcga cagtcggacc gcgttgcgcg cgggcggatc
     2701 gtcgggaccg gcggtcgtgc ccggtgactt ggacgctagc ctgctctacc aggccatcac
     2761 cgcggccgag ggcgctgtgc cgatgccccc cacggggagg ctctcgccgg cggaaatcgc
     2821 cgatttccgt cgctggatca cgataggcgc gcctgatcct cgcgagaaac cggcaaccgc
     2881 gacgtcctcg cccgctcggg tcgcggccgc acaaagccac aattggtggt cgctcaggcc
     2941 cgtcgagcaa ccttcggttc catcgtctgc caaggcaacg gccgattggc ccaggaaccc
     3001 gatcgacgct ttcatcctgg cgaagctcac agaaaaaggt ttcacgcccg cccccgaggc
     3061 cgaccgcagg accttgattc gccggctctc gtttgattt
//
LOCUS       DATAQL010001059        10107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4379059, whole genome shotgun sequence.
ACCESSION   DATAQL010001059 DATAQL010000000
VERSION     DATAQL010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4379059"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..662)
                     /locus_tag="VHS97_18245"
     CDS             complement(402..662)
                     /locus_tag="VHS97_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450201.1"
                     /translation="MADYTPYQRKIIDRYYKNLDAIQFQRLSELATDLFLAEGKKRDR
                     LWTRVEESLRKLEFPEKRIELLLQRRDPAQLVGILKELESGG"
     gene            complement(935..2029)
                     /locus_tag="VHS97_18250"
     CDS             complement(935..2029)
                     /locus_tag="VHS97_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450202.1"
                     /translation="MFAFLDLSNAGQPEVVAGFSPNQPPLPSNMSSNGPNPLAPKPYE
                     VAQAIPNASEPQSGIPTFGTLLTNNTGNVYLFNSAAHPNLEFSITNFSQLFQQETGHA
                     LTPTSVIGIGAFAGSNSDGGINDAHFPENTFTLAQATVPVTPGNPTPSPPILINPHEH
                     RIIDTSHRDLVRVSVFGTSGFPVSAINPATVKLDGVAPIAHFTRKVRRDEFPFETFVF
                     VANELKLPSGLTPATLTGQTFGGASFVTSKDVLNIPHSAKAFGQLKKFMGNASYYKSL
                     AKFEAKHPSAAISDSSTPVTAVSANPTPVGNAAIQVNYTPKVSAAGNAAKAETVKVRP
                     VVSIKRADARAAKSSVPTRVRYSMEHYLSK"
     gene            2797..3684
                     /locus_tag="VHS97_18255"
     CDS             2797..3684
                     /locus_tag="VHS97_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450203.1"
                     /translation="MVGRNALRCCLAVVALLGARSATASPINFTGNVANDFNPATNPG
                     VEIITDSFHQNNPSHLAQPSWMTAAGLVSGWNIKNVALDYNAATDTMYVGVQTYGVAG
                     NVDGNGTPGTPDPRLTAAGGTDPANFGGDKAMAVAFAPLTGTFNPSKPPTPVIVAGVS
                     GDKATQTGPGLDGFNVTKYAGGTTPGTVNLLTGFGKTLTAGMGNLAFDPSSAHPGFEF
                     TITNFSKILGANPANGLVVMTQDGSVNSVVTGKDQVVGTYPTNEAQTIPEPTTFLVWA
                     GLAASAMAWRYRRSRRTYA"
     gene            4054..4674
                     /locus_tag="VHS97_18260"
     CDS             4054..4674
                     /locus_tag="VHS97_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450204.1"
                     /translation="MPSESSRESLTMRDHVMFLVVMSLVSSFPLIPAADGQESKPAQR
                     HRGDVATIADGETSLRMTPGIVCRSIAGYEDYEPLPEAAQTAEEKLLVYFRPLGHRIE
                     QVDDFYQAHLVPDFQIRKRGEKAVLRQKLKFYEYKPKSKEPPRSIYMKNVIALKGLPP
                     GDYDLTIILHDELAKGPPANQVVKFRIIPPIDIEKPEKAKAGQSED"
     gene            complement(4746..5891)
                     /locus_tag="VHS97_18265"
     CDS             complement(4746..5891)
                     /locus_tag="VHS97_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450205.1"
                     /translation="MIIKYVLPILAVAGLVFAINRVVEARRPGPTAEPIVEPPTRPTS
                     VKMIAGSGLVEARRENIPIGVNIPGVVTEVFVKKGEKVKVGAPLFRTDDREFNSMLAV
                     REAELASSQAQLHKLIASPRPEDIPPAKAAAQEAEARMADAEAALARTERLFQRQMIA
                     ASDYDKDRYAYYAAKATYAKAKADLEKILAGSWKEDIEIARAAVQLAQSQVQSIKTSL
                     ERLIVRAPMDGEVLQLNVRLGQFAAMTWKEPMIVLGDSKRLHVRIDIDEPELLYFSKG
                     AEAIATLKGRPNIRFPLKFVYVEPYVIPKQSLTGNNSERVDQRVLQVIYELPDDRPLD
                     VYIGQQVDVYMKAATISKSIDSDMGLGNGRLPFEEETPSSQRGKPDA"
     gene            complement(5943..6713)
                     /locus_tag="VHS97_18270"
     CDS             complement(5943..6713)
                     /locus_tag="VHS97_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3450206.1"
                     /translation="MVTHLAPDVMMLDDVGLAPKGPLPTAVRIRGLSKHFGSGEQRVQ
                     ALKDIDWDVYTGQMTLIVGPSGCGKTTLLSVIAGILNCDSGEVEIFGQEVTMMSDRTL
                     TRFRAQNIGFVFQQYNLLPALTAVENAAIPLVIAGWTKYRAVKKAGEVLSSLGMGKKL
                     ESLPRQLSGGQMQRVAIARALVHEPRLLVCDEPTAALDHESGITVMELMRAAAVRPDR
                     AVVVVTHDNRVFGFGDRIAHMDDGAMVRVEERTRDTIS"
     gene            complement(6772..7971)
                     /locus_tag="VHS97_18275"
     CDS             complement(6772..7971)
                     /locus_tag="VHS97_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3450207.1"
                     /translation="MSWIAIKMLVGDKAKFLGIVMGLTFAALLINQQGSIFCGLMLRT
                     CGQITDITGADLWVMDPAVRFIDDVKPMLENNLYRVRGVEGVHWAVPLYKGTARAKVN
                     PDVARGNQKTVIEQVILLGLDDTSLVGAPHTSKILAGNLRDLRQPDAVIVDNVRLEKL
                     YPEENWNELAKEGQGFYQRFLRRQFEMNDHRAIIVGVCVATPTFQSNPVVYTTYARAK
                     SFAPQERKILSYVLAKTMPGVPPATVARRIAASTGLGAMSSDEFMTKTIDYYLRYTGI
                     PFNFGITALLGFLVGTAIAGQTFYNFTIENLKQFGALKAMGMTNLRIVGMILLQATVV
                     GLLGYGIGVGLAAFFGWRVTGTELAFFTPWQLLPLTAAAIVLICVLASLLSVQKVIRL
                     EPAIVFR"
     gene            complement(7988..8653)
                     /locus_tag="VHS97_18280"
     CDS             complement(7988..8653)
                     /locus_tag="VHS97_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HEX3450208.1"
                     /translation="MEEQDPTKVRLIDAAGEEFADKGFDATRIRTICDRAGANVAAVN
                     YHFGDKEQLYVETVLDAHRCSMELGEYDAAVAAGPAEQLRSFIHHFLTRVLAINHPQD
                     WRHRLMLREMLQPTSASDVLVRESIRPHFEQLQQVMRQFCPEADERKLNALTFSVIGQ
                     CLHYKMARPVTERLIGPEGFGALDLEFLTDHITSFCLAALGSIPPLNQAGLAATAGAV
                     ANV"
     gene            8904..9917
                     /gene="holA"
                     /locus_tag="VHS97_18285"
     CDS             8904..9917
                     /gene="holA"
                     /locus_tag="VHS97_18285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX3450209.1"
                     /translation="MNALEWLSAAEKPLIRPVYAVYGDDSYLIRESIRAIALALFADD
                     EERESGISRFPGPTSSLANVLDEVCTLPFFSRRRLVIVDDADPFVTKYRRDLEAYVAH
                     PSDSGTLVLQVKQWTATTKLAALVEKAGASINCASPRERELASWLTQLARTRHDAQLS
                     ADAARLLIELAGAEAGILAAEVEKLAVYVGESGKIERADIVKMVGAGRVETIWNTLDA
                     ATTGEARIALEHLDNLLAAGELPTPMLAAMTASLAKVHHAGRLRAAARLPLDEACRIA
                     GIPSFAVEKTRKQHAHLGPGRVDRLPGRLLKADLDVKGGSTLDPRTILEILLVELSLP
                     RTD"
BASE COUNT         2071 a         3014 c         2994 g         2028 t
ORIGIN      
        1 aaaagcgcga tcggataact caaaaattta caaaaccctc gtttccacgt caagcttttc
       61 atggcaagaa ttaaaaggcg atccggccag atcgcgcgtg tttaacatta agcgcttgac
      121 cttcaatggt taaatactat caatctcttt cgcgccgccc taacgaaggc ctcattaggc
      181 acacgctaat tctgcgaatg tgtaggaacc ggcgagtaag gtcggacaga gacatgatcg
      241 tctggctttc gatcgcgaac tgcctaatgc gatcggtagc gatttgatag gtggacctgg
      301 gaagaacttg acggaccaag atcacgctga acgcttacga acggtgctga aatgcgcgcc
      361 agcgcgtacg agccatgtct ggacgtgtgc gagcgatcag gctagccccc gctctcgagt
      421 tctttgagaa taccgaccaa ctgggcggga tcgcgtcgct gcagcaagag ctctattcgt
      481 ttctcgggaa actcgagttt cctcaagctc tcctcgactc gggtccagag ccggtcgcgt
      541 ttctttcctt cggcaaggaa gaggtctgtt gccagctcag ataggcgctg aaattggatg
      601 gcgtcgaggt ttttgtagta tcgatcgatg atttttcgct ggtaaggcgt gtagtcggcc
      661 actctacggc tctcctcgct ttcggcctcg ggcaaccggc acccgcggcg ctgccattac
      721 tttccggacc gctcgaattg ttggcccacg tcctaaaatc agaaccactc gcgcgggctc
      781 ggtcaagtct acggccgccc gcttcgctgt gactggcgtg ttaacgcccg cggccgaacg
      841 aggaactcac cagtgacgac gtggttaagt cgagctggaa cgatcgcgat gcacctgtgg
      901 ccagcgacgt cgccgccgct agccagagca ggatttattt gctcaggtaa tgctccatgc
      961 tgtaacgaac tcgggtggga acgctgcttt ttgcggcgcg agcatcggcc cgtttgatcg
     1021 agacgacggg ccgaaccttg actgtctcgg ccttggcggc gttgcccgcg gccgagacct
     1081 tgggtgtgta gttgacctgg atcgcggcgt tgcccacggg cgtcggattc gccgagacgg
     1141 cggtcacggg cgtgctcgag tcggaaatcg cggccgatgg gtgcttggcc tcgaatttcg
     1201 ccaggctctt gtagtacgag gcgttcccca taaacttctt cagctggccg aacgctttgg
     1261 cggaatgggg aatgttgagc acatctttag aggttacaaa cgacgcgccg ccgaatgttt
     1321 ggcccgtgag ggtggctggg gtcaagccgg agggaagctt gagctcgttg gcgacgaaga
     1381 caaaagtttc gaatgggaat tcgtcgcgtc ggaccttgcg ggtaaagtgg gcaatgggtg
     1441 caacgccgtc gagcttgacg gtggcgggat tgatcgccga aacggggaag ccggatgtgc
     1501 cgaagaccga cacccgcacc aaatcgcgat ggctggtatc gatgatccgg tgttcatggg
     1561 gattgatcag gatcggcggc gagggcgtcg ggttgcccgg cgtcacaggc accgttgcct
     1621 gggcgagcgt gaaggtgttc tcggggaaat gcgcatcgtt gatgccgccg tcagagttgg
     1681 acccggcgaa cgcaccgatt ccgatcacgc tcgtgggcgt gagcgcgtgg ccggtctcct
     1741 gttgaaagag ttgcgagaag ttcgtgatcg agaactcgag gttcgggtgt gccgcagaat
     1801 tgaaaagata cacattgcct gtgttgttgg ttagtagtgt accgaatgtc ggtatgcccg
     1861 actgaggttc gctggcattg gggatcgcct gcgcgacctc ataaggcttt ggcgccaacg
     1921 gatttggtcc gttcgacgac atgttggacg ggagtggcgg ctggtttgga gagaatccag
     1981 cgacaacctc cggctgaccg gcgttggaaa ggtcgaggaa ggcgaacatc tgaatgtcct
     2041 gacccccgag cgctggaaac tcagtgaaga atgggtctat tgctaataca gccgggttga
     2101 cattcccggc gttgccatta tcatccgcat ctcccgcgat cacaggtccg gtcggattcg
     2161 acgcggtgtt ggcgggaggc tgttcgaacc caatactcaa ggtgtcagtg gtggaatcgt
     2221 aagaaaccgc aatccccgaa atgtcgtaac ctgaagtcgg tataagcggt gttgcggtga
     2281 atggcggatt aaagaacatt gtatcggggt gtacgatccc cggtgtgttt gaagagttga
     2341 agatgacaac gcctggtgat tgtgtagcgg gaaaatcggt ggcgacgttt cccgtgaagt
     2401 taacgctaag taatcgccga tgatcaagtt gttccacggc cactgcggaa cgcttcgcgg
     2461 tcttaagacg atcgcggtgg cgccaagctc gagcagcctg gatcaacttg gagatcaagt
     2521 acatcgcccc tcatcctttc ttgactggtc cgccccgacc ggaacggcgc ctgactggaa
     2581 agggttcccc gagcactcag tgaacggatc accaaggatc aagacaaccc ggcggtatga
     2641 atcaaatggc cgacccggag atgcccgcca gggccggctt atgctgtttg gtttttctcg
     2701 gccactaaag tatcgtggtc gaccccagtg aaaccgccgg cgctcggcgg cgggtaaaat
     2761 tgggacgaaa gagctttcag aggaaggagc ctcgaaatgg taggccgaaa tgcgttgcga
     2821 tgttgcttgg ctgtcgtcgc actgcttgga gcccgctccg ctaccgcgag cccgattaac
     2881 ttcaccggca acgtcgccaa cgacttcaat cccgcgacga accccggtgt agaaatcatc
     2941 acggacagct ttcaccaaaa caatccaagt cacctcgccc aaccgagctg gatgaccgct
     3001 gccggactcg tgtccggttg gaacatcaaa aacgtcgcgc tggactacaa tgccgccacc
     3061 gacaccatgt acgttggtgt ccaaacctac ggtgtcgccg gcaacgtcga cggtaacggg
     3121 acgcccggaa cgcccgaccc caggttgacc gccgcgggcg gcaccgaccc ggcgaatttc
     3181 ggtggtgaca aggcgatggc agttgccttc gcgccactta cgggaacctt taacccgtcc
     3241 aaacctccca ctcctgtcat cgttgcgggt gtatcgggcg acaaagcaac gcagaccgga
     3301 cccggtctcg acgggttcaa cgtcaccaag tacgcgggtg gcacaactcc cggcaccgtt
     3361 aacttgctga ccggtttcgg caaaacgctc acggcgggga tgggcaatct tgcattcgat
     3421 cccagttcgg cgcaccccgg attcgagttc acgatcacca atttcagtaa aatcttgggc
     3481 gccaaccccg cgaacggatt ggtggtcatg acccaggacg gctcggtcaa cagcgttgtt
     3541 accggcaagg accaagtggt cggcacttat cccaccaacg aggcccagac gattcccgaa
     3601 ccgacgacct tcctggtctg ggcgggtctg gcggccagtg caatggcgtg gagataccgc
     3661 cgctcgcggc ggacctacgc atgacgttcg ttgcctcgct ctgatcaaca ccataaacgc
     3721 atccctgcgc aagatggcgg tcgtaccctg ataccgtcac actgtagatc ggcctggaat
     3781 ggcggcgaag tgcggccgtt ttcttccagg ctttcgcagt ttcccctccc gtcccgaact
     3841 cacagactag tcatcgccaa gcattcgacc ctcattgctc atcctcgccg tcaggctcgc
     3901 ggcgggtacg gagccgacgg tgccgcagcc gaaggatcta cccacggtcg cggttcggcc
     3961 atggagtgca atcatccggc ttctcctcga accgccgctt gcgaatttgg tgacggtcag
     4021 ttaatttggc gctctgtcca ccgggataag gcgatgccga gcgagagcag ccgcgagagt
     4081 ctcacgatgc gcgaccatgt gatgttcctg gttgtcatga gtctcgtgtc gagttttcct
     4141 ttgattccgg ccgcggatgg ccaggaatca aagccggccc agagacatcg gggagacgtc
     4201 gcaacaatcg ccgacgggga gacgagtctg cgcatgacac cgggcatcgt ttgtcgatcg
     4261 atcgccggct acgaagatta cgagccgtta cccgaggcgg cccagaccgc cgaggaaaag
     4321 ctactcgtct actttcggcc gttggggcac cgcatcgagc aggtcgacga tttttatcag
     4381 gcccatctgg tgccggactt tcagatccgc aagcgaggcg aaaaggccgt tctccgccag
     4441 aagctgaaat tctatgaata caagccgaag tccaaggaac cgcctcgttc catctacatg
     4501 aaaaacgtga tcgcgcttaa gggcctaccg ccgggagact acgacctgac gatcatcctc
     4561 cacgatgagc tcgccaaggg ccctcccgcc aaccaggtcg tcaagtttcg aatcatcccg
     4621 cccatcgaca tcgaaaagcc ggaaaaagca aaggccggcc agtctgagga ctaaacggct
     4681 ttgcatgaat ccgtacagca ttacaaccgc gtcgggctgg aaagcccgac ctacttgggc
     4741 gcggatcagg cgtcgggctt gccgcgctgg ctggaggggg tttcctcctc gaagggaagc
     4801 ctgccattcc cgaggcccat gtcggagtcg atcgacttcg aaatcgtggc ggccttcatg
     4861 tagacgtcca cttgttggcc gatgtaaacg tcgagcggcc tgtcatccgg cagctcgtag
     4921 atcacctgca ggacgcgctg atcgacccgc tcggaattgt tgccggtcag gctctgcttg
     4981 ggaatgacgt acggctcaac gtacacgaac ttcaagggaa agcgaatgtt gggacgaccc
     5041 ttcagggtgg cgatcgcttc ggcacccttg gaaaaataga gaagttcggg ctcatcgata
     5101 tcgatccgga catgcaggcg cttgctgtcg cccagcacga tcattggctc tttccaggtc
     5161 atggcggcga actggcccag ccggacgttg agctgaagga cttcgccatc catgggggcg
     5221 cgaacaatga gtcgctcgag gctggtcttg atgctctgga cctggctctg ggcgagctgg
     5281 acggcggccc gagcgatttc gatgtcttcc ttccagctac ccgccaggat tttctcgagg
     5341 tcggccttgg ccttggcgta ggtcgccttg gcggcgtaat aagcgtagcg gtccttatcg
     5401 taatcgctgg cggcgatcat ctgcctctga aacagccgct ccgtccgcgc gagcgccgcc
     5461 tcggcgtcgg ccatcctggc ctcggcctcc tgcgcggctg ctttggccgg cggaatgtct
     5521 tcgggccgcg gcgacgcgat cagcttgtgg agctgcgcct gggacgatgc gagctcggcc
     5581 tcgcgcaccg cgagcatcga attgaactcg cgatcgtcgg tcctgaagag cggagcgccg
     5641 accttgacct tctcgccctt cttgacgaaa acctcggtga ccacgcctgg gatattgacg
     5701 ccgatcggga tattttcccg gcgcgcttcg accaggcctg aacccgcgat catcttgacc
     5761 gaagtgggcc gggtcggcgg ttcgacaatc ggctctgccg tcggccccgg ccgtcgcgct
     5821 tcgaccacac ggtttatcgc gaaaaccaac ccggccacgg ccagaatcgg tagcacatac
     5881 ttgatgatca tgaccgcacg gctcctcaaa atcgttcaaa acatcctatt aacacacata
     5941 tatcacgata tagtatcacg ggttcgttcc tcaacgcgga ccatggctcc gtcgtccatg
     6001 tgcgcgatcc ggtcgccgaa gccgaacacc cggttgtcat gcgtgacgac gacgactgcc
     6061 cgatcggggc ggaccgccgc cgcgcgcatt aactccatga ccgtaattcc gctctcgtgg
     6121 tcgagcgccg cggttggctc atcgcatacg agcagacgag gctcatgcac aagcgccctg
     6181 gcgatcgcca cgcgctgcat ctgacctccc gaaagctggc ggggtagact ttcgagtttc
     6241 ttacccatgc cgagcgacga gaggacctct ccggcctttt tgaccgctcg atacttggtc
     6301 cagccggcga tcaccagggg aatcgccgcg ttctcgacgg ctgtcaaggc cggcaagagg
     6361 ttgtactgct gaaagacgaa gccgatgttc tgggctcgaa acctcgtcaa ggtgcggtcg
     6421 ctcatcatgg tgacctcttg accgaagatc tcgacttcac cgctatcgca attgaggatg
     6481 ccggcgatca ccgacaaaag agtggtcttg ccgcagcccg acggaccgac gatcaaggtc
     6541 atctgaccgg tgtagacgtc ccagtcgatg tccttgagcg cctgcacgcg ctgttcgccg
     6601 ctaccgaaat gcttactcaa accacgaata cgcaccgcgg tcggcagagg gcccttgggc
     6661 gcgagcccca cgtcatcgag catcatcacg tcaggcgcca gatgcgtgac catcaattca
     6721 tccctcgatt cgattggaaa tcagcaacca gctcgcatta agcccttttg ctcaacggaa
     6781 tacgatcgcc ggttcgagac gaattacctt ttgcacggag agcaggctcg ccaagacgca
     6841 aatgaggaca atcgccgccg ccgtcaaggg cagcaattgc cagggtgtga agaaggccag
     6901 ctcggtcccc gtcacgcgcc atccgaaaaa ggccgccaag ccgacgccga tcccgtatcc
     6961 cagcaaacca acgaccgtgg cctgcaacag gatcatcccg acgattcgca ggttggtcat
     7021 tcccatcgcc ttcaaggcgc caaactgctt gagattttcg atggtgaagt tgtaaaaggt
     7081 ctgaccggcg atcgccgtcc ccaccagaaa accgagcaag gccgtgatcc cgaagttaaa
     7141 gggaataccg gtatatctca agtagtaatc gatggtcttt gtcatgaact cgtcactact
     7201 catcgctcct aagccggtgc tcgccgcgat ccgccgcgcg accgtcgcgg gaggcactcc
     7261 cggcatcgtc ttggccagta cataagagag gatcttgcgc tcctggggag cgaacgactt
     7321 ggcccgggcg tatgtcgtat acacaacggg gttcgactgg aacgtcgggg tcgccacgca
     7381 cacacccaca atgatcgccc gatggtcgtt catctcgaac tgccggcgga gaaaccgctg
     7441 ataaaacccc tggccctctt tggcaagctc attccagttt tcttcggggt agagtttctc
     7501 cagccggaca ttgtcgacga tgacggcatc gggctgccgc agatcacgca ggttgcccgc
     7561 gagtattttc gaggtgtgtg gcgcgcccac caggctcgtg tcgtcgaggc ccagcaggat
     7621 cacttgctcg atcacggttt tttgattgcc acgcgccacg tcggggttga ccttggcacg
     7681 cgcggtccct ttgtagagtg gaacagccca gtgaacacct tcgacgccgc gcacgcggta
     7741 caggttgttt tccagcattg gcttgacgtc gtcgataaaa cgaacggcag gatccatcac
     7801 ccaaaggtcg gcgccggtga tgtcggtgat ttgaccacac gtcctgagca tcaagccgca
     7861 gaaaatcgag ccctgctgat tgatcaataa ggcggcaaag gtcagcccca tgacgatgcc
     7921 caggaacttg gccttatcgc ccaccagcat cttgatcgca atccacgaca aagtgccacc
     7981 tccggcatca gacgttggcg accgccccgg cggtagcggc caagccagcc tggttcaacg
     8041 ggggaattga accaagagcc gccagacaaa aggacgtgat atgatccgtg aggaattcca
     8101 ggtcgagcgc tccaaacccc tccggcccga tcaagcgctc ggtgaccggc cgcgccatct
     8161 tgtagtgcag acattggccg atcaccgaga acgtcagagc gttgagcttt cgctcatcgg
     8221 cctcgggaca gaattgcctc atgacctgct ggagttgctc gaaatgcgga cgaatcgact
     8281 cgcggacgag cacgtccgac gctgaggtcg gctgcagcat ctcccgcagc atcagccggt
     8341 gccgccaatc ctggggatgg ttgatggcca gcacgcgagt caaaaagtga tgaatgaaag
     8401 aacggagctg ctccgccggt ccggccgcga ctgctgcatc atattcgccg agctccatgc
     8461 tgcaacgatg ggcatccagg accgtctcaa cgtacagctg ttccttgtcg ccgaagtgat
     8521 agttcaccgc cgccacattt gcgccggcgc gatcgcagat cgtgcggatc cgcgtggcat
     8581 caaatccctt gtcggcgaac tcctcgccgg ccgcatcgat caagcgaacc ttcgtgggat
     8641 cttgttcttc cataacaccg ccttacctct tacccgcttg attgcgcacg ccggttgcgc
     8701 cgctccggac cactgtcgcc catcaccaat tcatcgcaaa cagttgtttc aaacggacgt
     8761 tttaagcaac tgtatacatt ttcggacacg ggtcaaggtg ctcttgaacg caattctgta
     8821 gcgacaatga cgattgcgcc cgggtatgtt gggaccgggg ctgttgctcc gagctctttc
     8881 gtccgccagc agggcaatca ggcatgaacg cactggagtg gttgagcgcg gctgaaaagc
     8941 cgctgatccg gccggtatat gccgtttacg gcgacgactc ttacctgatc cgagagtcga
     9001 tcagggcgat cgcgctggcc ctgtttgctg acgacgagga acgagagtca gggatcagcc
     9061 ggtttcctgg ccctaccagc tcgcttgcca atgtactcga cgaggtttgc accctcccct
     9121 ttttttcccg ccggcggctg gtgatcgtcg acgacgccga cccgttcgtg acgaagtatc
     9181 gccgcgatct ggaggcgtat gtggcccatc cctcggattc tggaaccttg gtcctgcagg
     9241 tcaagcagtg gactgcgacc accaagctgg ccgcgctcgt cgaaaaggcc ggtgcatcaa
     9301 tcaactgcgc aagcccgcgc gagcgcgagc tggcgtcgtg gctaacgcag cttgcgcgga
     9361 cgcggcatga tgcccaactg agcgccgatg ccgcgcgtct cttgatcgag ctggcgggag
     9421 cggaggcggg cattttggcg gccgaggtcg aaaagctagc tgtctacgtc ggcgagtcgg
     9481 ggaaaatcga gcgggcagac attgtcaaga tggtcggagc cggccgcgtg gagacaatct
     9541 ggaatactct ggacgccgcc acgacgggcg aggctcggat cgcgctcgag cacctcgaca
     9601 acctgctcgc cgccggtgaa ctaccgacgc ccatgctggc ggcgatgacc gcgagcctgg
     9661 cgaaagtgca tcacgccggc cggctccgag cggcggcccg tctccctctc gacgaggcgt
     9721 gccgcatcgc cgggattccc tcgtttgccg tcgaaaagac ccgaaaacaa cacgcgcatc
     9781 tcggccctgg ccgcgttgac cggcttcccg gcaggctcct gaaggccgac ctcgacgtga
     9841 aggggggcag cacgctcgat ccgcggacga tcctcgagat cctgctcgtg gaactcagcc
     9901 ttccgcgcac cgactgaatc gtgactaatg actaagacag ccactttctt tcgcacattt
     9961 gagcagcgat cgagcaaccg cgggcacttg tccgctgttt tcctccgccg accggatcgg
    10021 cccgcatttg tgccggtcaa gcgagttttc gaggaattca taatttcagc gcaaaaacaa
    10081 aaatcggcgc gcccattgcg ttattcg
//
LOCUS       DATAQL010001060         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1327567, whole genome shotgun sequence.
ACCESSION   DATAQL010001060 DATAQL010000000
VERSION     DATAQL010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327567"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..1506
                     /locus_tag="VHS97_18290"
     CDS             358..1506
                     /locus_tag="VHS97_18290"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3450210.1"
                     /translation="MTTKIKTESILDRYDPLREILCCPVTKEPLRLVEIDELLPCLSE
                     AERGRVPEGTIGAFVSNAAGRAYPLTERVADFLAEDSLAIRPPPPATAPTSPADDVKR
                     SVKDWYDRFGWRKNKRGMYLDSALFSQNRPVGHGLYEMTSHLSIMDRLAGGDFVLDAA
                     SGAIAHPEYLAYSWFFRSRVCVDMSTTALMEADAKLRPSDFCCLADICQLPFRDESFD
                     GAVSGYTIQHIPESQQGRAVEELYRVIRPGAHLCILTDVTYTRGHRLLVLALRAMRKL
                     SRVLRMASPRPDPSASPTGSREEAPHALYSHVRGLAWWKGVARELTHRCRIESLRLLN
                     KWEFESLYGGSNRAAKALRWIEGALPRSMSGPSAYCVIDICKPASGHE"
BASE COUNT          259 a          479 c          547 g          290 t
ORIGIN      
        1 cctctccgtc cgccgtcggc tccaggtcca tgccgcgccg gccacctgct accgattgtc
       61 ggggccacag ccattcgtgt caatcggcaa tttacaacct ttgcttgaac cgaatctcgg
      121 gattgaccga agtagactag acggatatcg tgccgccgat atttctcatc cgtggggcag
      181 atgtgcttcg tgagtctcga ggagcgcgat cggggaacgg aacaacgggg acgttcccaa
      241 atcttggcgc aagcctggcg gtttgggtaa ttagtcggtt gtcgggtcgt tgaattaata
      301 tgctcccggc ccggcatggg gccggggaca acagcgggcg cggggggccg atgccaattg
      361 acgaccaaga ttaagactga atcgatcctc gaccggtatg accccctccg ggaaatcctc
      421 tgctgcccgg tcaccaagga gccgctccgc ctagtcgaga tcgacgaact gttaccttgc
      481 ttgtccgagg ccgagcgggg gcgggtcccg gagggcacga tcggcgcctt cgtctcgaat
      541 gccgccggtc gggcgtatcc cctgacggag cgggtggcgg atttcctagc cgaggattcg
      601 ctggcgatcc gacctccccc tccggcgacc gcgccgacga gcccggccga cgacgtcaag
      661 cggagcgtga aggattggta cgaccgcttc ggctggagga agaacaagcg gggcatgtac
      721 ctggactcgg cgctcttctc ccagaacagg ccggtcgggc acggcctcta cgagatgacg
      781 tcgcatctgt cgatcatgga tcgcctagcc ggaggagatt tcgtcctgga cgccgccagc
      841 ggcgccatag cgcacccgga gtatctggcg tactcctggt tctttcggtc gcgggtctgt
      901 gtcgacatgt caacgaccgc gctgatggaa gccgatgcga agctgcggcc gtcggatttc
      961 tgctgcctgg ccgatatctg ccagctcccg ttccgcgacg agagcttcga cggagccgtc
     1021 tccggctaca cgatccagca tatcccggag tcgcagcagg gccgggccgt cgaggagctg
     1081 taccgggtca tccgtccggg ggcgcacctc tgcatcctca cggacgtgac ctacacccgg
     1141 ggacatcggt tgctggtcct ggcgttgcgg gcgatgcgga aattgtcgcg ggtgctgcgg
     1201 atggccagcc cgcggcctga tccgtccgcc tcgccgacgg ggtcgcggga agaagcgccc
     1261 cacgcgctgt actctcatgt gcggggactg gcttggtgga agggggtggc gagggagctg
     1321 acgcatcggt gccggatcga gtcgctgcgg ctgctgaaca agtgggagtt cgagtcgctg
     1381 tacgggggct cgaaccgggc ggcgaaggcc ctgcgttgga tcgagggggc cctcccgagg
     1441 tcgatgtccg gcccgagcgc ctactgcgtg atcgacatct gcaagccggc gtctgggcat
     1501 gagtaggccg gctggcatcg ggagccaaga gggccgtcgt atcggtcgcc gccgggcgat
     1561 tacgtggtgg agatg
//
LOCUS       DATAQL010001061         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4379599, whole genome shotgun sequence.
ACCESSION   DATAQL010001061 DATAQL010000000
VERSION     DATAQL010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4379599"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHS97_18295"
     CDS             <1..223
                     /locus_tag="VHS97_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX3450211.1"
                     /translation="DLEKGAEFHLTFDDGFRELVVYTPSNQPDVIAVEPYTQTTDAIN
                     LQSRGLGAGLRVLAHGQQDNFLITMETVG"
     gene            533..1060
                     /locus_tag="VHS97_18300"
     CDS             533..1060
                     /locus_tag="VHS97_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450212.1"
                     /translation="MAATQELLMEPILAAGHDDLHGLRLDLLRSATEIYLGLAYPGVV
                     VPEVVRRRLVWRDGCAADELMSAAPFERAGKAPGRPAPIYALRLGNHRYPHMKLQIQP
                     WPNDAGFMLSVNSHDQVSGIDLGAVDAMAFRELQAENQRLKESIEEAWDDAGLPTFLR
                     YLRDYIQSRGTDPGH"
     gene            1358..2791
                     /locus_tag="VHS97_18305"
     CDS             1358..2791
                     /locus_tag="VHS97_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450213.1"
                     /translation="MDLTTLPLVWAQAQTPPQPAAPNPNSIYFLALRRWCSSLTLSSP
                     QAIPSLHPLWAFVAGLAGLLILAVLAQGPIRVLQQLFDLVGHARLVQKSFRRLWRAGR
                     MVAIAIGFTVLAWTASQAWIFNQESRKADLISLTKSRRLSELALEQGLFASLTPLRDV
                     AGLADNLPLLVIAAIVVFRSSFEIPGWGVPSPGTGTISTQARTRAGWSTLIWGCCSLY
                     ALYRIVARMAGSNDLPLGSCLVVEGLIVPLLMVVCDGFLLAWVVVELRNAGFDDTGED
                     RLDTRQAIALMPAAALVCAFALPARYVATLVLLASSYLPTSASATSLGNYVRWQLGWG
                     LTDVQVVGLIGFGLAGAVAWSRGSIGGAMAGYGRLLAFEGGHVVALAAIAGFASGLLA
                     ASAYAVVLLLPAQSWVLAAADSYAHFATLPVGLCALAALIELAERSLPTATLYRSSTR
                     RTAAVTDAQSDRSTHDDALSPVASPII"
     gene            complement(2804..2965)
                     /locus_tag="VHS97_18310"
     CDS             complement(2804..2965)
                     /locus_tag="VHS97_18310"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450214.1"
                     /translation="MWQLDDARDLGDSEAQLLIDYPRLKAVNLVDAWDYAQDHPDEIA
                     AQIRQNEVA"
BASE COUNT          573 a          915 c          990 g          690 t
ORIGIN      
        1 cgatttggag aagggggccg agttccatct cacatttgac gatggctttc gcgaactcgt
       61 tgtttacacg ccctccaacc agccggatgt tatcgccgtc gaaccgtaca cgcagacaac
      121 cgacgcgatc aatctgcaat cgcgcggctt gggcgcgggc ctccgcgtgc ttgcgcacgg
      181 gcagcaggac aactttctta tcacgatgga aactgtgggt taagttgagt ttgcgtgcgt
      241 ccccctgtgg aatggacaaa gggaaatttg aacaaattcc tgatgttgag cgtcgaataa
      301 cttaacggtg ccaaaggcga gtgatctcga ttacgccagt gcagcgtggt cacggatgcg
      361 aaggaatcac gtcaatcact acgcggttcg tcgcatttga gggaacacgt gaaccgcaag
      421 gtggccggcg gataggttcg atgctctacg gcatttgacg cactggcgcc ttgaccacta
      481 tccgatcttg gtggactgta aatcgaagtg gataaccggc ggggaccttc cagtggcagc
      541 aacccaggaa ttgctcatgg agccgatctt ggcggcgggg catgacgacc tgcatggttt
      601 gcgtctcgat ctgttgcgca gcgcaacgga gatttacctg ggactagcgt atcccggggt
      661 tgttgtgccc gaagtcgtca ggcgccgctt ggtctggcgc gacggttgtg ccgccgacga
      721 actcatgagc gcggccccgt ttgagcgagc cggcaaggcg cctgggcgcc cagcacctat
      781 ctatgcgttg cggctcggga atcatcgcta tccgcacatg aaactccaga ttcaaccgtg
      841 gcccaacgac gcggggttca tgctttcggt caacagccat gaccaggtct ccggcatcga
      901 cttgggcgcg gtcgatgcca tggcattccg tgagctccag gccgagaacc agcgactgaa
      961 ggaatcgatc gaagaggcct gggacgatgc cggattgccc accttcctgc gttacctccg
     1021 cgactacatc caaagccggg gcaccgatcc tggtcattag tgattagtca ttggtcaatg
     1081 gtcgttcgtc attggtggct ctcaccggga ctccgagcgg gaccgaaatt gcaacggctg
     1141 acatgaccac gcgaagctca gtgtccgatc accaataact attgaccaat gactattcac
     1201 cgagagtcga gtacaatcgt cgtacgtcgg gcgattttgc cgatactcgg atagcgaaag
     1261 agcccggaga aattgatcgg gatggctggc ccgcgggttg tcgcattaaa catcaggccg
     1321 cgggcgagcg acgaacgaca gcgggagagc ggccttcatg gatctgacga cgctgccctt
     1381 ggtctgggca caggctcaaa cgccaccgca accggccgct ccgaacccca actcaattta
     1441 ctttctggcg ctacggcggt ggtgctcgag cctgacgtta tcctctcctc aggcgattcc
     1501 tagccttcac cccctgtggg cattcgtggc aggcctggca ggcctgttga ttctggccgt
     1561 tctcgcgcaa gggccaatcc gggttttgca gcagctcttt gatctcgtcg ggcacgcgag
     1621 actggtccag aaatcatttc gacggctgtg gcgcgccggg cgcatggtgg cgattgcgat
     1681 cggtttcacg gtgttggcgt ggacggcgag ccaggcctgg attttcaacc aggagagtcg
     1741 aaaggccgat ttgatctcgc tgaccaagtc gcgccggctg agtgagctgg cgcttgagca
     1801 gggacttttc gccagtctaa caccgctccg cgacgtggcc gggctggcag acaacctgcc
     1861 acttctcgtc atcgcggcga tcgtagtctt caggtcgtca ttcgagattc cgggctgggg
     1921 tgttccgtcg ccggggactg gaacgatcag cacgcaggct cggacgcgcg ccggctggtc
     1981 gaccttgatc tggggatgct gttcgctgta tgcgctttac cgaatagtag cccggatggc
     2041 cggcagcaac gatcttccgc tgggcagttg cttggtcgtc gagggcctga ttgtcccgct
     2101 tttgatggtg gtgtgcgacg ggttcttgct cgcctgggtg gtggttgaac tgcgcaacgc
     2161 cggtttcgac gataccggcg aagaccggct ggacaccagg caagcaatcg cactcatgcc
     2221 tgcggcagcg ctcgtgtgcg ctttcgcctt gccggcccga tacgtagcga ccttggtatt
     2281 gctggcttcg tcatatctgc ccacatcggc cagtgccaca tctctgggca actatgtccg
     2341 ctggcaactc ggctggggat taaccgatgt tcaggttgtg ggactcatcg ggtttggcct
     2401 ggccggagcg gtcgcctgga gccggggatc gattggcggc gcgatggccg gatacgggcg
     2461 gctcttggcg ttcgagggtg gccatgtcgt cgcgctggcg gcgatcgccg ggttcgcctc
     2521 gggattgctg gccgcgtccg cgtacgctgt cgtattattg ttgccggcgc aaagctgggt
     2581 cttggccgcg gccgacagct acgcccactt cgccacgctg ccggtagggc tttgcgccct
     2641 ggccgcgttg atcgagctag ccgaacggtc gttgccgacg gcgacgctat atcggtcctc
     2701 cactcgcagg actgcggcgg taaccgatgc ccaaagcgac cggtccacac acgacgatgc
     2761 cctctcaccg gtcgcgagtc cgataatttg aggatgcctc aggtcatgcg acttcattct
     2821 gacggatctg ggcggcgatc tcgtcgggat ggtcctgagc atagtcccag gcgtcgacca
     2881 aattcaccgc tttcaggcga gggtagtcga tcaggagctg cgcttctgaa tctcccaagt
     2941 cgcgggcgtc atcgagttgc cacactggga tccgctcccg ctacttccgt gagtgcctgt
     3001 ccggtccgat acgtcgatgg tacctcaatt ggacgcatct cggaactctt gatgcgtgcg
     3061 atcgcgcatg ccgctgtgga tccaccggaa tttttgccga aaatgacatt tccgagtcga
     3121 attgagccct tctccttcga ctaccggata gtggaggtga attgcctt
//
LOCUS       DATAQL010001062         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5298752, whole genome shotgun sequence.
ACCESSION   DATAQL010001062 DATAQL010000000
VERSION     DATAQL010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5298752"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /gene="rpsF"
                     /locus_tag="VHS97_18315"
     CDS             complement(<1..375)
                     /gene="rpsF"
                     /locus_tag="VHS97_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX3450215.1"
                     /translation="MNTYEGMFLLDSTKAATAWEDTVKHVHDILTKHHSEIVASRQWD
                     ERRLAYPIDGHKKGSYLLTYFRTEGENLHEIVADCGLSDIILRELVLKVHPKLADHLV
                     NQAMTSTPNVDAENGIGEDELDD"
     gene            complement(437..1021)
                     /gene="pth"
                     /locus_tag="VHS97_18320"
     CDS             complement(437..1021)
                     /gene="pth"
                     /locus_tag="VHS97_18320"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX3450216.1"
                     /translation="MATIKLVVGLGNPGSKYQGTRHNIGFELVDRLARGGSTASFSSK
                     FEGQLADIEIDFRRVLLLKPETFMNLSGRSVGQAVRFYKLSLADVLVVCDDLSLPVGK
                     LRLRPGGSDGGQKGLRDITAHLGTDQFPRLRIGIGANDDIDAVDYVLSRFRSAERTAI
                     DDSLILASQAVAVWVTQGIDAAMNRFNGTPTSKQ"
     gene            complement(1117..1773)
                     /locus_tag="VHS97_18325"
     CDS             complement(1117..1773)
                     /locus_tag="VHS97_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX3450217.1"
                     /translation="MADAAKIRVETRDPAKNKGTGSRVSRRLRANGRIPAVIYGHKQA
                     VVPITVGRDDVWRMIKTPGHLADLELGESTETVLIREVQWDHLGKEILHLDFSRVSAE
                     EVIETEVTLELRGQAAGIANGGILEHLVHRLSINCRAGAIPDSIKADVSHLQIDQGIH
                     VRDLSLPPDVTVNADPDLLLVHVVVRGTQEEPAAEAAAEGTTQPEVIKPERKDKEKED
                     "
     gene            2417..>2605
                     /locus_tag="VHS97_18330"
     CDS             2417..>2605
                     /locus_tag="VHS97_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3450218.1"
                     /translation="MAGLNGQVALVTGASRGVGRGVAVGLARAGATVFATGRSIKQTE
                     FGAGIVSIVCDHTDDQAVD"
BASE COUNT          504 a          819 c          746 g          536 t
ORIGIN      
        1 gtcgtcgagc tcatcctcac cgatgccgtt ctcggcgtcc acattcggcg ttgatgtcat
       61 cgcctgattg accaggtggt cagccagctt gggatgcacc ttgagcacca actctctcag
      121 gatgatgtcg ctcaagccgc agtcggcgac aatctcgtga aggttttcgc cctcggtccg
      181 aaagtaagtc agcaaatagc tgcccttctt gtggccgtcg atcgggtacg ccagccggcg
      241 ctcatcccac tggcggctgg cgacgatctc ggaatgatgc ttggtcaata tgtcatggac
      301 atgcttgacc gtgtcttccc aggccgtggc cgccttggtg ctgtccagca ggaacattcc
      361 ttcataggta ttcacgggca agactaggtt cctcccaacg agaacgtaac gggcgaaaaa
      421 cgtcgaggcg ggccgctcat tgcttactgg tcggcgtgcc attaaagcgg ttcatggcgg
      481 cgtcaatccc ctgggttacc cagacggcga cggcctgcga ggccaggatc agagagtcat
      541 cgatggcggt tcgttcggcg ctgcggaatc ggctcagcac gtagtcaacg gcgtcgatgt
      601 cgtcattggc gccgatacca atcctcagcc ggggaaactg atcggtgccg aggtgggccg
      661 tgatatcgcg cagtcctttt tgaccaccat cggaaccgcc gggacggagc cgcaattttc
      721 ccacaggtaa gctcagatcg tcgcacacca cgaggacgtc cgccaatgac aacttgtaga
      781 aacgcaccgc ttggccaacc gagcggccac tcaggttcat gaatgtttcg ggcttcaaca
      841 gcaacacacg ccggaaatcg atctcgatgt cggcgagttg accttcgaac ttgctcgaaa
      901 acgaagcggt cgaaccgccg cgcgccaggc gatcgaccaa ctcgaagccg atattgtgtc
      961 gtgttccctg gtacttgctg ccgggattgc ccagaccgac caccaatttg atcgtcgcca
     1021 acgccacggc tcctggcagt cgtcccagag cgaatcttcg cgatcatgcc ccggcgagcg
     1081 cagctagcct ggagctgaac acgagaacga cctcgatcaa tcttcctttt ctttatcctt
     1141 gcgctcgggt ttgatgacct ccggttgtgt ggttccctcc gccgcggcct cggccgccgg
     1201 ctcttcctga gttccgcgca cgaccacgtg aaccaacagc aggtccggat cggcattcac
     1261 cgttacgtcc ggcggcaggc tcaagtcgcg gacgtggata ccttgatcga tctgaagatg
     1321 actgacgtct gccttgatcg aatcgggaat cgcgcccgcg cggcagttga tgctgaggcg
     1381 atgcacgagg tgctcgagga tgcccccgtt ggcgattccg gcggcctgac cacgcaactc
     1441 aagcgtaact tcggtctcga tgacctcctc ggcgctcacg cgactgaagt caaggtggag
     1501 tatctcttta ccaaggtgat cccactgtac ctctcggatg agcacggttt cggtcgactc
     1561 gcccagttcg aggtcggcga gatgcccggg cgtcttgatc atccgccaga catcgtcgcg
     1621 accgaccgtg atgggcacga ccgcctgctt gtgaccgtag atcaccgcgg ggatccgccc
     1681 gttcgcacgg agtcgccgtg agaccctgct gccagttccc ttgtttttcg cggggtcgcg
     1741 cgtctcgacc cggatcttcg ctgcatccgc cattgcccac tcactttagc cggccctcgc
     1801 gttccgccga ggtccgtaga aacacgaccc gaaaccgatc tcagaacacg gcttctggaa
     1861 ccgcgggaat ccaccattat gagaatcatg ccgattttgt tcaagtcccc gcgacagaat
     1921 caaatcagtc gtccactggc ccctgctctt ttcgactgcc cgccgcacgg tgatggcttc
     1981 gttcgtcaag attcttggcg gcaccggaca gcttcgccga ttccgtgtgg atgaaaacca
     2041 ggaaggcacg cacaccctga ccggcaattt cgcggtcgag ttcacaaagt taggtcaaaa
     2101 aacgagtggc acttttcggc actaattggc actcctgggg acgtgcccag tgccaattcg
     2161 ccttggccca agcggatgcc gctcaggcac aggccgctaa tgcacaatgc tttacgtcga
     2221 tcggatccgc ccctgtccag tgcccgtcgg ttgacttttc agagtcctga ccgcgaatgt
     2281 ggacgtcggc accagcagcg gcacacggct ctgatcaggg aattgcacgc atagaaaccg
     2341 ccctcgtccc ggtcgagtcg cactcgtcta ctcaacgctt gttagacacc caacgccaga
     2401 cagaggtaca gcaaacatgg cgggcttaaa cggtcaggtt gcactcgtga ccggcgccag
     2461 ccgtggcgtg gggcgcggcg tggcggttgg cttagcccgc gccggagcga cggtgttcgc
     2521 gaccggccgc tcgatcaaac agaccgagtt tggcgctgga atcgtctcga tcgtctgcga
     2581 ccatacggac gatcaagccg tcgac
//
LOCUS       DATAQL010001063         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3925085, whole genome shotgun sequence.
ACCESSION   DATAQL010001063 DATAQL010000000
VERSION     DATAQL010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3925085"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VHS97_18335"
     CDS             complement(<1..959)
                     /locus_tag="VHS97_18335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3450219.1"
                     /translation="MSITGTATASAATSLVGPLEERHLRSRMYRNAADSTRLTSTHWH
                     IAWANGLGWGFDGMDGAILALVAPMLMKEFAIDLGTYRSGLQISGLVGMVGLYLWPWL
                     ADKFGRRNILALNIALFSLAMPLVALSPGWGSFVAIYSIVRIALSGEWAVGSMLVVET
                     WPARLRGVVVSVDRSAWGVGAALAGSLVTFVVTEWGWRAAYVLPVAVALLAVYVRLLC
                     PESPYWVRTQDRKHRIRERQSAGFALDEGDRQWIVKTEQPSWRQLFLPDLRRNTVMAT
                     LVASLALVSYSTVGLWMPLFLSQQHGWSTAEYGSFYIWWALFAT"
     gene            1180..1509
                     /locus_tag="VHS97_18340"
     CDS             1180..1509
                     /locus_tag="VHS97_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450220.1"
                     /translation="MSTTRRNCRLNLMGTLVVSALSLCALALPLSSAKAQCLGVDLGI
                     GCAGLGVGSWYSAHPYYYGYPNYYAAPYYAPAYYAPPRYYAPPPNYVDPGYSYTPAGR
                     PDYGNEQ"
     gene            complement(1651..2046)
                     /locus_tag="VHS97_18345"
     CDS             complement(1651..2046)
                     /locus_tag="VHS97_18345"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3450221.1"
                     /translation="MLVGGGTSQGAAAIPLSTAENSVYAPENCRVGIKNLVEISLLNA
                     SGRAGRQRKSGARCAGRQRTGSGIQRHGLSLVEELRTLFGRAGAAGEIELCPGVHHGF
                     AFPQRWCYDKPAAERHWERLIALYRWRLG"
     gene            complement(2135..>2536)
                     /locus_tag="VHS97_18350"
     CDS             complement(2135..>2536)
                     /locus_tag="VHS97_18350"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3450222.1"
                     /translation="TSFQMIRNARARGIPVLSGSDTGNASAFAHGRWHGKEAELFVRE
                     IGMSPMEAILANTSGNAAMVNLAGEVGVVARGMLADIVIWDSDPIADMTVLQRPEEIS
                     AIIKDGKVIDRGTVGGFSRLHEELGRSRTLS"
BASE COUNT          512 a          840 c          736 g          448 t
ORIGIN      
        1 gtcgcgaaca gcgcccacca gatgtagaag ctgccgtatt cggcagtcga ccagccatgc
       61 tgctgcgaca ggaacagcgg catccacagg cccaccgtgc tgtaggagac gagcgccaag
      121 ctcgcaacca aggtcgccat caccgtgtta cggcgcaggt cgggcaggaa tagttgccgc
      181 cagctgggct gctcggtctt gacgatccac tggcggtcgc cttcatcgag cgcgaaaccg
      241 gcgctctggc gctcgcggat ccggtgtttg cgatcctggg tgcgcaccca atagggcgat
      301 tccgggcaca acagccggac atagaccgcc aacagggcca ccgccaccgg aagcacatag
      361 gccgcccgcc acccccactc ggttacgaca aatgttacca gggagccggc aagcgctgcg
      421 ccgacccccc aggctgagcg gtcgacgctg accacaacgc cgcgtagccg agccggccag
      481 gtctccacga ccagcatcga gccgaccgcc cattccccgc tcaacgcgat ccgaacgatg
      541 ctataaatcg cgacgaaact accccatcca ggagagagcg cgaccagcgg catcgccagg
      601 gagaacagcg caatgttgag cgccagaata tttctccgcc caaatttgtc cgcgagccag
      661 ggccagagat ataggccgac catgccgacc aatcccgaga tctgcagccc actgcggtag
      721 gtgccgagat cgatcgcgaa ctctttcatc aacatcggag cgaccagggc caaaatcgcc
      781 ccgtccatcc catcgaatcc ccaaccgaga ccattggccc acgcgatgtg ccaatgcgtc
      841 gaggtcagcc gcgtcgaatc cgccgcgttg cggtacatcc gcgaccgcag atgacgctcc
      901 tccagcggac cgaccaagga ggtcgccgcc gatgcggttg cggttccggt gatgctcatg
      961 gttctccctc tgcggccctc gttcccgaag agcagcctga gccgcagccg ccgaggcgtc
     1021 aagttttggg gacaccttgt tgtgtcatag ggtacaggcc gacccggaac gccggatcgc
     1081 agactgacga cggataaaag tcatcctcgc ggaaccccac agacaaccca actgttatcg
     1141 aagaggatgc tagttttcga ctagaatttg gaggtgatta tgtcgaccac tcgaagaaac
     1201 tgccggttaa atctgatggg aacgctggtc gtctctgcct tgagtctgtg tgcactggcg
     1261 cttccattaa gctcggcgaa agcgcagtgt ctaggcgtcg acctgggcat cggctgcgcc
     1321 ggccttggag tgggatcgtg gtattccgcc catccgtatt actatggcta tccgaactac
     1381 tacgccgcgc cctactacgc cccggcctac tatgccccgc cgcgctatta cgccccgccg
     1441 cccaattacg tagatccggg gtatagctac accccagctg gtcgtcccga ttacggtaac
     1501 gagcaataag ggaaatcggc acgacgtcgg tgggtccggc accggcggct ttggtcaacc
     1561 agacgacggc ttgtgatctc ggataatccg agagccttct cacccatata gggggaatca
     1621 tttgtcgccg cctggttgcc agcgacgccg tcagcccaag cgccaccgat agagcgcgat
     1681 cagccgctcc cagtgccgct ccgcggccgg cttgtcgtag caccagcgct gtgggaaggc
     1741 gaagccgtga tggacgccgg ggcagagctc gatctcgccg gcggcgcccg cacgaccaaa
     1801 cagagtgcgc aactcctcga ccaagctcaa accatgccgc tgaattccac tgccggtccg
     1861 ctgccgaccg gcgcaacggg cacccgattt ccgctgacgg ccggctcggc cagatgcatt
     1921 caagaggctg atttcgacca gatttttgat gccgacccgg caattctcgg gtgcgtaaac
     1981 agagttttcc gctgtcgaca gcggaattgc ggcggcgccc tgcgacgttc cgcctccgac
     2041 cagcaaatcc tggttaagtg gctgcgaact cgcgaaggcg tgtccgagcc aaacgacccc
     2101 ggggagattg tccttaggtg atcggcttta agtgctaaga gagtgtcctg gaccgaccaa
     2161 gttcttcgtg caacctgcta aagccgccaa ctgtcccgcg gtcgattacc ttgccgtcct
     2221 tgatgatggc cgagatctcc tcgggacgct ggaggacggt catgtccgcg atcgggtcgc
     2281 tatcccaaat gacgatgtcg gcgagcatgc cgcgcgcgac caccccgacc tcgccggcca
     2341 ggttgaccat ggccgcgtta ccgctggtat tcgccaaaat cgcctccatc ggcgacatgc
     2401 cgatttccct gacgaacaat tccgcttcct tgccgtgcca ccgtccgtga gcaaaggccg
     2461 aggcgttgcc cgtatcactg ccgctcagta ccgggatacc gcgggcccgt gcgttgcgga
     2521 tcatctgaaa gctggt
//
LOCUS       DATAQL010001064         3940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4991768, whole genome shotgun sequence.
ACCESSION   DATAQL010001064 DATAQL010000000
VERSION     DATAQL010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4991768"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2521
                     /locus_tag="VHS97_18355"
     CDS             <1..2521
                     /locus_tag="VHS97_18355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3450223.1"
                     /translation="DGWIYACHGFSNSSQVQGKDKRSISMNSGNTYRMRPDGSHAEYF
                     THGQVNPFGLAFDALGNLYSCDCHSRPVYQLIRGAFYPSFGKPDDGVGFGPDMVTHDH
                     GSTGIGGISFYAADQFPEAYRNTVFVGNVVTNRVNHDRINWHGSSPQGIEQPDFIWSE
                     DNWFRPVDLELGPDGALYIADFYNRIIGHYEVPLTHPGRDRERGRVWRVVYRGIDGKP
                     APLPVMTDRTRSSSDELIADLAHPNLVVRIAAANQLVDRGGDSAIAAVRKIMSPGMPS
                     HQRVHGLWILERQGKLDDATLAACAADSDRELRVHSMKLLVDRPELGRPLTELTLTHL
                     SDADPFVRRAAAEVLGTHPSAANIKPLLSLRQSTSAADTHLIHVARIALRDQLKLPET
                     WSRLDDLGLTERDRRDIADVATGVPDSHAAAFLLAQLHRHHEAAGTLLRFAHHIARYG
                     SPPAVVEAVGLATDEKRTPVERLNLLKSMQEGAQERGAGLDDTARKLASQLARQLLGS
                     SQPGEITLGIDVVRDFRFRDLDPDIRNVIGRHELPAAPRMHALAALTAIDPKANLAAV
                     GNVLADAAAPMAMRERAANLLGNLDLAEAKTVLLANLPSAPERLQSAIAQALVRRRPG
                     ADSLLQAIAAGKASARLLQQRPVTISLESSGLPQVTERITALLKGLPPADEKLAALYA
                     SRRHGFQTAKADPALGAQVFEKSCGICHQIAGKGAKVGPQLDGIGSRGLDRLMEDVLD
                     PNRNVDQSFRVTTLALNNGQVVSGLLLREEGEVSIVADSQGKEVRVPKSAVEERSIAP
                     MSPMPANLTDQISADDFYTLMAYLLARREPTNTPPGNAGSK"
     gene            2616..2975
                     /locus_tag="VHS97_18360"
     CDS             2616..2975
                     /locus_tag="VHS97_18360"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX3450224.1"
                     /translation="MSEAKIIKTGSTPRIEGSRITVFDVLDYHKHGWQPSDIADLFNL
                     STRQIEVAIEYIETHKTEVMATYQRILDRHAKGNPPELQARLDEGHERFLAMVKARRE
                     AKIGPMLKRPPKDCWTI"
     gene            complement(3046..3900)
                     /gene="larA"
                     /locus_tag="VHS97_18365"
                     /pseudo
     CDS             complement(3046..3900)
                     /gene="larA"
                     /locus_tag="VHS97_18365"
                     /inference="COORDINATES: protein motif:HMM:NF044708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
BASE COUNT          796 a         1301 c         1133 g          710 t
ORIGIN      
        1 cgatggctgg atctatgcgt gccacggctt cagcaacagc tcgcaggtcc agggtaaaga
       61 caagcgttcg atcagcatga actcgggcaa cacctaccgc atgaggcccg atgggtcgca
      121 tgccgaatac ttcacccacg ggcaggtcaa tccgtttgga ttggcgttcg atgcactggg
      181 caatctctat tcgtgcgatt gccacagccg gccggtttat cagctcatcc ggggggcgtt
      241 ttaccccagt ttcggcaagc ccgacgatgg agtcggtttc ggtcccgaca tggtcaccca
      301 tgaccatgga tcgacgggta tcggcggcat tagcttttac gcggctgacc agtttcccga
      361 agcttatcgc aacacggtct ttgtcggcaa cgtcgtgacc aacagggtca accacgaccg
      421 gatcaactgg cacgggtcgt cgccccaagg aatcgaacag cccgatttca tctggagtga
      481 ggacaactgg tttcgacccg tcgacctcga gctcggcccc gacggcgcac tctacatcgc
      541 ggacttctac aaccgcatca tcggccacta cgaagtaccg ctcacccatc ccggtcgcga
      601 tcgcgaacgg ggacgcgttt ggcgcgtcgt ctacagagga atcgacggaa agcccgcgcc
      661 gcttcccgtc atgaccgatc gaacaaggtc gtcgagcgac gagctgattg cggacttggc
      721 ccatccgaac ttggtcgtgc ggatcgcggc ggccaaccag ctcgtcgacc gcggcggcga
      781 cagcgcgatt gccgctgtcc gcaaaatcat gtcgcccggt atgccctcgc accagcgggt
      841 ccatggcctt tggattctcg agcgacaagg caaactggac gatgccaccc tggccgcctg
      901 cgccgccgac tccgaccgcg agctgcgtgt ccacagcatg aagctgctcg tcgaccggcc
      961 cgagctcggt cgcccactga cggagctgac cttgacccac ctgagcgatg ccgacccatt
     1021 cgttcgccgc gccgccgcgg aggtcttggg cacccatccc tccgccgcca acatcaaacc
     1081 cttgctctcg cttcggcaat cgacgtcggc ggcagacact catctcatcc atgtcgcccg
     1141 gatagcgctg cgagaccagt tgaaacttcc cgagacttgg tctcggctcg atgacctcgg
     1201 cctgaccgaa cgcgatcggc gcgacatcgc cgacgtggcg accggcgtgc ctgacagtca
     1261 cgcagcggcg ttcttgctcg ctcaactcca tcgccatcac gaagccgcgg gcacactgct
     1321 gcggtttgcc catcacatcg ctcgttacgg atcgccgcct gcggtggttg aggcggtcgg
     1381 tctggcaaca gatgagaaac ggaccccggt ggaacggctc aatctcttga agagcatgca
     1441 ggaaggggct caggaacgtg gcgcagggct cgacgatacg gcgcgcaagt tggccagcca
     1501 actggcccga cagcttctcg gatcgtcgca accgggcgag atcacgcttg gcatcgacgt
     1561 cgtccgtgac ttccggttcc gcgacctcga ccctgatatc aggaacgtga tcgggcgcca
     1621 cgaacttccc gcagcgcccc ggatgcacgc cttggcagcc ctgacggcca tcgatcccaa
     1681 ggccaatctc gccgccgtgg gcaacgtgct cgccgatgcg gcggcgccga tggcgatgcg
     1741 tgagcgggca gccaacctgc tgggcaacct cgacctcgcg gaggccaaga ccgtcctcct
     1801 ggccaacctc ccgagcgcac ccgagcggct ccagtcggca atcgcccaag ccctggtgcg
     1861 ccgccgcccg ggagccgatt cccttttgca agccatcgcg gccggcaagg cgtcggctcg
     1921 cttgctccag cagcggccgg tcaccatcag cttggaaagc tcgggcctgc cgcaagtcac
     1981 cgagcgtatc accgcattgc tcaaaggact ccccccagcg gacgagaagc tcgcggccct
     2041 gtacgccagc cgccgccacg gctttcaaac tgccaaggcc gacccggcgc tgggcgccca
     2101 agtgtttgaa aagagctgcg ggatctgcca ccagatcgcg ggcaaaggcg ccaaggtcgg
     2161 cccgcagctc gacggcatcg gcagtcgcgg cctcgatcgc ttgatggaag acgtcctcga
     2221 ccccaatcgc aacgtcgatc agtcgtttcg ggtcacgacc ctcgcactca acaatggtca
     2281 ggtcgtcagc ggcctgctct tgcgcgaaga gggcgaagta tcgatcgtcg cggactcgca
     2341 aggcaaggaa gtccgtgttc ctaaatcggc cgtcgaagaa cgatcgattg ctccgatgtc
     2401 gccaatgccc gcaaacctga ccgatcagat ttctgccgac gacttctaca cgttgatggc
     2461 ctatttgctc gcgcggcgcg aaccgaccaa tactccgccg ggcaatgcgg gatcgaagtg
     2521 atagacagaa tgccgggtct aaatcgtggc tttgacgggg cgctggggga agtccataat
     2581 caatggtgat cgtctcacca ttgaggtaac gagttatgtc agaagcaaag atcatcaaaa
     2641 ctggaagtac gccccgaatt gagggctcgc ggatcacagt tttcgacgta ttggactatc
     2701 acaagcatgg ctggcagccg agtgatatcg cggacctttt taacctttcg acacgtcaga
     2761 tcgaggttgc gatcgaatac atcgaaacac ataagacaga ggtcatggca acctaccagc
     2821 gaattctcga tcgccacgca aagggcaatc caccggaact tcaagctaga cttgatgagg
     2881 gtcatgagcg tttcctagcc atggtcaagg cgcgacggga agccaagatc ggtcctatgc
     2941 tgaaaagacc gccgaaagac tgctggacta tttgacacga atcgacgact tccgcggcgc
     3001 cgggcggatc tacgtaccct gatatgctcg ctacttaggc actcctcatc ccatcagcac
     3061 aggtaacaca taaggcccct tgggaatcac cgcaacctga gcctcgggcc cgtattccgc
     3121 cagcgaatct gccagcgctt tttcgacggt cggagccgac tcgacaaagc acgcggacag
     3181 gacatcgggc ggcaagccgt ccgagacgat cttgacgcgc gccttgcggc gcacctttgc
     3241 tagctcttcg agctgccact ggtccatcac gaaatagtcc cgccccagaa tccgctccat
     3301 gaagacctcg agcgacgggt tttcccggaa caacgactga aattctgcgc tgcctaatcc
     3361 ctcctacagg cttgccgcca gaatgatcgt ccctcccact ttgacaatcg gcaggcagcc
     3421 ggtgagccct ttgaccgcct ggtaaaacgt agtatctagc ggataacccg cggagctcgt
     3481 gaccacgatt tcaaccggct tcggcacaag tgcgcggcag acgcccgcca tgaagcgaac
     3541 accttcaagg aaggcctgtt ccatgtcacc ggccaccacc gacgtgaccc gccgctggct
     3601 gtcgagcgtc acgttgacga tgaagtcgca gccggccatg cgggcgatcg ccgtgttctc
     3661 ctcatgcacc gggttaccct cgaggatgcc gcaatcggcc ttggggtgtt ccagcagctc
     3721 gggcccgtgc caccgtttga cggtttctag tgcggccacg ccgggacaaa tcagcttgcg
     3781 accccccgaa taaccggcca tcagatgcgg ctcgatcagg cccgtcgcga tcttcaagtc
     3841 cgcccggacg taacggctgt cgatccatcc gggaatcccc cgcgcggtcg tgccgaccag
     3901 cgtatgctcg tcaaggcgcg tcccatagtg gtcctcgact
//
LOCUS       DATAQL010001065         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2548797, whole genome shotgun sequence.
ACCESSION   DATAQL010001065 DATAQL010000000
VERSION     DATAQL010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2548797"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VHS97_18370"
     CDS             <1..592
                     /locus_tag="VHS97_18370"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX3450225.1"
                     /translation="ASLPIAAVTALQALRDSGALREGGTTLINGAAGGVGHFAVQIAR
                     ALGAVPTGVCGPANVDFVKSLGAETVIDYSRDDFTRRSERYDVVFDAVAKSSFGACRH
                     LLKLGGCYVTTVPSPGILPWIPVQSIAGLFGRVKKAKFLMVRPEGSDLAYLGTLADEG
                     RLRPTIGRTFPLERAREGHEASEVGHTRGKIVLEVG"
     gene            complement(598..1617)
                     /locus_tag="VHS97_18375"
     CDS             complement(598..1617)
                     /locus_tag="VHS97_18375"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3450226.1"
                     /translation="MSTTRIGSTGIGMTILLTVVSSVVAEDDPFTNHKEPPVVGRWDI
                     TVHGPDGDYPSWLEVTLSGYRTLVGSYVGRTGSARPIAHVIADGNAIKFTIPPQWERR
                     TDDLHFTATLDHGILRGETTDETGRRLTWEGNRAPSLARTHVSSWGEPVDLFNGHDLT
                     GWRPRNGTSKNGWVVRDGILTNATPGNDLVTERKFDDFKLHAEFRYPKGSNSGIYLRG
                     RYEMQVEDNYGDEPESHKIGGIYGFLTPSLNASKPAGEWQTVEATLVGRTLTVVLNGE
                     RIIDRQAIPGPTGGALDSHEAEPGSLMLQGDHGSVEFRKLTLIPAATVEGPRGPESAG
                     TNRRE"
     gene            1886..3439
                     /locus_tag="VHS97_18380"
     CDS             1886..3439
                     /locus_tag="VHS97_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX3450227.1"
                     /translation="MILQKGAVMKIRTDRRRFLRASATGGALLGLGDLSFLSRLRPVS
                     ADEATLDPKVVRLQPGIEPLVRLLEETPRARLLEEVAARVRKGTAYRDVLAALMLAGV
                     RNVQPRPSVGHKFHAVLVVNSAHLASLSSPDSDRWLPIFWALDYFKSSQAQDVREGNW
                     TMGPLDESAVPPARKARKAFVAAMDGWDESAADAAVASLARSAGSNEVYELFCRYGAR
                     DFRSIGHKAIYVANSWRTLQCIGWQHAEPVLRSLAYALLMHEDGNPATRDDPADLPWK
                     HNQERADRVNEGWTGGTPDTAAAADLLAMLRTGASDDACEQVVELLNREVSPQSVWDA
                     LFAGAGELLLRQPGIVSLHAVTSTNALHFAYQASGDDRTRRLLMLQNAAFLPMFRAAM
                     DGRGKVADARIDQLEPVATTQVGPGALEEIFAEAGRDRMIAAGKTLAYLQAGCDARPL
                     IDAARRLVFLNGDDPHDYKFSSAVLEDYSQASPAWRDRYLASSLLLLPRPADRDNSLV
                     QRTRAALEA"
BASE COUNT          572 a         1098 c         1152 g          631 t
ORIGIN      
        1 ggcctcactg ccgattgccg cagttacagc acttcaggcc ttgcgcgaca gtggcgcgct
       61 acgcgaggga ggtacgaccc tgatcaacgg cgccgcgggg ggtgttggcc acttcgccgt
      121 ccagattgcc agggccttgg gggcagtgcc gacgggtgtt tgcggcccgg ccaacgtcga
      181 tttcgtgaag tcgctcgggg ccgagacggt aatcgactac tcccgagatg acttcactcg
      241 ccgctccgag aggtacgacg ttgtcttcga cgccgttgcc aagagcagtt tcggggcctg
      301 ccgtcacctg ctcaagctgg gaggctgcta tgtcacgacc gtcccatcgc caggtatttt
      361 gccctggatt cctgtgcagt cgatcgcggg attgttcggc cgagtgaaga aggcgaagtt
      421 tctcatggtc cgtcccgagg ggagcgacct tgcctacctg ggcactctcg ccgatgaagg
      481 ccgcctccgg ccaacaatcg gccgcacgtt tccgctggaa cgtgcccgcg aggggcacga
      541 agcgagcgaa gtcggacata cgcgcggcaa gatcgtgctc gaggttggat agggctctta
      601 ttcccgacga ttcgtccctg ccgactccgg gccgcgcgga ccttcgaccg ttgcggccgg
      661 gataagcgtc agctttcgga actcgacaga accgtggtct ccctgaagca tgagcgagcc
      721 gggctcggct tcatgagagt cgagcgcgcc accggtggga ccgggaatgg cctgtcggtc
      781 gatgatgcgc tcaccgttga gtacgaccgt cagggtgcga ccaacgagcg tggcttccac
      841 ggtttgccat tcgccggcgg gcttggacgc gttgagcgac ggtgtcagaa atccataaat
      901 gccgccgatc ttgtggctct cgggttcatc accgtagttg tcttcgacct gcatttcata
      961 acgtcctcgc aagtaaattc cgctgttgct gcctttggga tagcggaact cggcgtgaag
     1021 cttgaaatcg tcgaacttgc gctcggtcac gaggtcgttg ccgggagtcg cattcgtgag
     1081 gattccgtcg cggacgaccc agccgttctt gctcgtgcca ttgcgcggac gccaaccggt
     1141 aaggtcgtgg ccgttgaaca agtcgacggg ctctccccaa ctggagacgt gggttcgggc
     1201 gagcgatggt gcgcgattgc cttcccacgt caggcgtctg cccgtttcat cggtggtctc
     1261 gccacgaagg atgccgtgat cgagcgtggc ggtgaaatgc aggtcgtcgg tccgacgttc
     1321 ccactggggt gggatcgtaa acttgatcgc gttgccgtcg gcgatgacgt gtgcgatcgg
     1381 gcgcgcgctg ccagttcggc cgacatatga gccgacgagg gtgcgatatc ctgatagtgt
     1441 gacttcgagc cacgatggat aatcgccgtc gggaccgtgg acagtgatgt cccagcggcc
     1501 gacgaccggc ggctctttgt ggttcgtgaa tggatcgtct tctgccacca ctgatgaaac
     1561 gacggtcagg agaatcgtca tcccgatgcc cgtcgaaccg atccttgtcg ttgacatttc
     1621 gttctccccc tcgagtgcag tattcaagag gcatttggtg tttcaggatc atccagcagg
     1681 cgcgattttg caatggcgaa aaggacatgc catgctttgg atggtcggct ggtaactgct
     1741 cattctggcc agcgcgccac gggggttatg tcgtgagccg ggcgcctcgg ttataatacg
     1801 tgtggaagtg acgaacacgg gcggcgcggt cggcccgcgc ccagaatacc gaactcggcg
     1861 gcaaacgagt cttcgaacgg caggattgat cctgcaaaaa ggagcggtca tgaaaatccg
     1921 aacggatcga cggcgcttct tgcgggcgtc cgcgacgggc ggcgcgctgc tcggccttgg
     1981 ggacctaagc tttctatccc gacttcggcc ggtctcggcc gacgaggcga ccctcgaccc
     2041 gaaggtggtg cgtctccagc ccgggatcga gcccctcgtt cgcctgctgg aggagacccc
     2101 ccgcgcgcgg ctgctggagg aggtggccgc acgggtgcgg aagggcacgg cttaccgcga
     2161 cgtgctcgcg gcactcatgc tggccggggt gcgcaacgta cagccgcggc ccagcgtggg
     2221 ccacaaattc cacgcggtcc tcgtagtgaa ctcggcccac cttgccagcc tcagctcgcc
     2281 cgacagcgac cgctggctgc cgatcttctg ggcgctcgac tacttcaaga gttcgcaggc
     2341 gcaggacgtc cgcgagggca actggacgat ggggccgttg gacgagtcgg ccgttccccc
     2401 cgcccgcaag gcccgcaaag cattcgtcgc ggcgatggac gggtgggacg agtcggcggc
     2461 tgacgcggcc gtggcttccc tggcccgctc ggccgggtcc aacgaggtct acgagctgtt
     2521 ctgccgctat ggcgcccgcg acttccgctc gatcgggcac aaggcgatct acgtggccaa
     2581 tagctggcgg acgctccagt gcatcggctg gcagcacgcc gagccggtcc tgcgctcgct
     2641 ggcctacgcc ctgctgatgc acgaggatgg caaccccgcc acgcgcgacg acccggccga
     2701 cctcccctgg aagcacaacc aggagcgcgc cgatcgggtc aacgagggct ggacgggcgg
     2761 cacgcccgac actgcggccg cggccgacct gctcgccatg ctccgcacgg gcgcgagcga
     2821 cgacgcttgc gagcaggtcg tcgagttgct caatcgcgag gtctccccgc agtcggtctg
     2881 ggacgccctg ttcgcaggcg cgggggaact gctgctccgt cagccaggga tcgtctccct
     2941 gcacgcggtg acttcgacca acgccctgca tttcgcctac caggccagcg gtgacgaccg
     3001 gactcgccgg ttgctgatgc tccagaacgc cgcgttcctc cccatgtttc gcgcggcgat
     3061 ggacggccgc ggcaaggtcg ccgacgctcg gatcgatcag ctcgaaccgg tcgcgacgac
     3121 gcaggtcggt ccgggggcgc tcgaggagat cttcgcggag gcaggccgcg accggatgat
     3181 cgccgccggc aagacgctgg cttacttgca ggccgggtgc gacgcgcgac cactgatcga
     3241 cgcggcgcgg cgtctggtgt tcctcaacgg cgacgacccc cacgattaca agttcagttc
     3301 ggccgtcctg gaagactaca gccaagcctc acccgcctgg cgcgaccgct acctggcctc
     3361 gagcctgctc ctcctgccgc gcccggcgga ccgagacaac tcgctggtgc agcggacccg
     3421 agctgcccta gaagcgtaga cagggcgagg gaa
//
LOCUS       DATAQL010001066         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1632158, whole genome shotgun sequence.
ACCESSION   DATAQL010001066 DATAQL010000000
VERSION     DATAQL010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632158"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHS97_18385"
     CDS             <1..235
                     /locus_tag="VHS97_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450228.1"
                     /translation="RTANETSNRLDSEARAARARLAELQHASDQRNAFAGDLEAVRLR
                     IEQLTVDFRQARIANEQLRALLNVFGLVDHLDS"
     gene            complement(286..2220)
                     /locus_tag="VHS97_18390"
     CDS             complement(286..2220)
                     /locus_tag="VHS97_18390"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3450229.1"
                     /translation="MTADQPGFLELPKDGCKGPQTSTEESGLSFDRSGSTAGMPRPAA
                     ENTVSPPSLIEMVKRDLTHQWEQGRQVSLETYLEAMPVLGTRETVSPDLILAEYRARA
                     RFGAMPDLSQYEERFPHQSGTLRELIDQQSQDPSTGSLLGQIDQMMGAPQNAPLSKVR
                     APKMQTRLPKRFGRYRRVKLLGRGAMGTVYLVHDTQLGRQVALKVPHFRWEEGGTLPE
                     HRELDRFYREVRVAATLDHPNLCPVYDAGEIDGIPYLVMAYIKGRPLSRYIKRSRPMS
                     QRRAAAIARKLALALEEAHSQGVVHRDLKPSNIMVNARRDLVIMDFGLVWRIGAQDER
                     LTRVGLVLGTPAYMSPEQICGRAEGLGPCCDIYSLGVILYELLTGCVPFEGPEAFVLG
                     QIVFAEPAPPSKHRPDVDPLIEAICLRAMAKKVEERYASMRELAAALERFLRARSNTF
                     RPAPKTARAEEIVAVMGTATETPDAESLAELARPPIEAPLVEASSAERTVPPTDLMPL
                     WLALHQWIEIIEHFVLQRSRRSVNHRNYDRLYRMLVETCRMRAGTTEGSTRQFYQRLE
                     DLVVPWMTPRAMECADREILGSLLCYCREAEQALRDRTKPLPDLGKKEEWVISWRVAA
                     VGITICVVIGLLIVGGLLWI"
     gene            2614..>3788
                     /locus_tag="VHS97_18395"
     CDS             2614..>3788
                     /locus_tag="VHS97_18395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3450230.1"
                     /translation="MEISGESNATNGAPPDLLPLIRRSGVLNARQLEEVSSKVQRGEY
                     PRDSHALAGRLVAEQILTEFQAKRLLRLKVHGLVVGRYVILDRLGAGAKGRVFRAQHR
                     LMGRVVALKIIAPHIASRASSIARFHREMRMIGRLDHPNVIRAFDADQIGELLYIVME
                     YVPGRSLDRVLEERGPIPPPLAVNYTAQAARGLAHAHERGIVHRDIKPSNLFLSDEGH
                     VKVLDLGLSALMEADTEASFATAAGFVVGTLHYMSPEQSAGIDVDARSDLFSLGCTMY
                     YLLSGRVPFPGDTVAECLARRLRGGLVPITELNSELSPRLVELLEKLLARRPEDRFQT
                     AAEAALALEALAHGEADVAPSHELAQQPAADAFVAEPALSGGVAPTTQPPSSSPSESP
                     "
BASE COUNT          667 a         1212 c         1167 g          742 t
ORIGIN      
        1 ccgaacggcg aacgagacct ccaatcgcct ggactccgag gcgcgagccg cgcgcgcccg
       61 cctcgccgag ctccagcacg cgagtgacca gagaaacgcc ttcgccggcg acctggaagc
      121 cgttcggctt cgcattgagc agcttaccgt tgattttcgc caggcccgca tcgcgaacga
      181 acagctacgt gcactcctga atgtcttcgg gctcgtggac cacctggatt cttgacccgg
      241 ctcggccgcg ctcggcgcgg cgccaaactc cggtctttga aaatatcaaa tccaaagcaa
      301 accccccacg atcaacaggc caatcaccac gcatatcgtg attcccacgg ccgctacccg
      361 ccagcttatc acccactcct ccttttttcc gaggtcgggc aatggcttcg ttcggtcccg
      421 cagggcctgt tcggcttcgc ggcagtaaca caatagactc ccgaggatct cccgatccgc
      481 acactccatg gccctcgggg tcatccaggg gacaaccagg tcctcgagtc tttgatagaa
      541 ctgcctggtc gatccttccg tggtgccggc gcgcatccgg caggtctcga ccagcatcct
      601 gtaaagcctg tcgtaattcc tatgattcac ggagcggcgc gaacgctgca ggacgaaatg
      661 ctcgatgatc tctatccact ggtgcaaggc gagccacaat ggcatcaggt cggtcggcgg
      721 gacagtccgc tcggcactcg aggcttcaac cagtggtgcc tcgatgggtg ggcgggctag
      781 ttcggcaagg ctctccgcgt cgggcgtttc tgttgcggtg cccataaccg ccacgatctc
      841 ctcggcgcga gcggtctttg gtgccggccg gaaggtgtta gagcgggcgc gcaggaagcg
      901 ttccagtgcc gcggccagct ctcgcatgga ggcgtagcgc tcttccactt tcttggccat
      961 cgctctcaag cagatggctt cgatcagggg atcgacgtcg ggccgatgtt tcgagggtgg
     1021 tgcgggctcg gcgaagacga tctgccccag cacgaacgcc tccggcccct cgaaagggac
     1081 acagccagtg agcagctcat agaggattac gcccaggctg taaatatcac aacaggggcc
     1141 gaggccttcc gccctgccgc agatctgctc gggcgacatg taggccggcg tgccgaggac
     1201 gagaccgaca cgggtaagcc gctcgtcctg ggcgccgatg cgccacacca ggccgaagtc
     1261 catgatgacg aggtcgcggc gagcgttgac catgatattg gagggcttca ggtcgcgatg
     1321 aacgacccct tggctgtgag cctcctccag cgccagggcc agcttgcgcg cgatggcggc
     1381 tgcccgccgc tgcgacatgg gccggctccg cttgatgtaa cgcgacagag gccggccctt
     1441 gatgtaggcc ataaccaggt acgggatccc gtcaatctca cccgcgtcgt ataccgggca
     1501 gagattggga tgatccaagg tcgccgcgac gcggacctcg cggtagaagc ggtccaactc
     1561 ccgatgctcc ggtagcgtgc cgccctcctc ccatcggaag tgggggacct tgagcgccac
     1621 ctggcggccc agctgcgtgt catgaaccag gtagactgtc cccatcgctc cccgtccgag
     1681 cagtttaacg cgccggtagc ggccaaatcg cttgggcagc ctggtctgca tctttggtgc
     1741 gcggactttc gaaaggggag cgttctgcgg cgcgcccatc atctggtcga tctggcccag
     1801 cagcgaaccg gtcgacgggt cctgcgattg ctggtcgatc aactcccgca aggttccgga
     1861 ctggtgagga aatcgttcct cgtactggga caggtcgggc atcgcaccga atcgagcccg
     1921 cgcgcggtac tcggccagga tcaggtcggg cgagaccgtc tccctggtcc cgaggacggg
     1981 cattgcctcc aggtacgtct ccaggctcac ctgacggccc tgttcccact ggtgagtcaa
     2041 gtcacgcttg accatctcga tcaggctggg cgggctgaca gtattttcag cggctggcct
     2101 gggcatgccg gcagtcgacc cgctccggtc aaacgacaga ccagattctt ccgtcgaggt
     2161 ctgcggcccc ttgcagccgt ctttgggtag ctcaagaaat ccaggctgat cggccgtcat
     2221 gggcggcttc ccaggagaag gccatgtacg tgattccggg tccttcgtgg actgcgctcc
     2281 attctagatc atacactaga atctcatgcg gacggggctt taacaagcct tgttgatact
     2341 cacggccgcc tggaaggcgg tcggcaggcg catggccttg gtcttcgaga gtgaccaatc
     2401 gcgacgttgg gagcacttgt tccgttcgcg cctgtcttcg gtaatccttc ccagtttgtg
     2461 ataaacaggc tgtcgagttg atcctgttcg cagtcgagtt tggcagatcc gtggcgaaag
     2521 acggctcgat tgtcccattg atcgattgtt agcgtgccga aatacgaccc gcgccctcca
     2581 attccagtgc cgagactttg ggatctacca gttatggaga tcagcggcga gtcgaacgca
     2641 accaatggcg cgcctccgga tctgctgccc ctcattcggc gctccggtgt actgaatgcg
     2701 cgtcagctag aggaggtgag cagcaaggtc cagcgcggcg agtatccgcg cgactctcat
     2761 gctctggccg ggcgtctggt tgcagaacag atcctaactg agtttcaggc caagcggtta
     2821 ctgcggctca aggtccacgg cttggtggtc ggccgctacg tgatcctgga tcgcttgggg
     2881 gcgggagcca agggccgtgt attcagggcc cagcatcgtc tcatgggccg agtagtcgcc
     2941 ctcaagatca tcgctcccca catcgcgtcg cgggcgagtt cgattgcccg attccatcgg
     3001 gagatgcgca tgatcggccg gctcgatcac cccaacgtga tccgcgcctt cgacgccgac
     3061 cagatcggcg agctgctcta tattgtcatg gagtatgtac cgggtcggag cctcgatcgc
     3121 gtgctcgagg agcgaggacc gatccctccg cccctagcgg tcaattatac ggctcaggcc
     3181 gctcggggtc ttgcccatgc ccacgaacgt gggatcgtcc atcgcgacat caaaccttcg
     3241 aatctgttct tgagcgacga ggggcacgtc aaggtcctcg acctgggctt gagcgccctc
     3301 atggaggcag atactgaggc ctcgtttgca accgctgccg gcttcgtggt gggcacactt
     3361 cattacatgt cgcccgagca aagcgccggg atcgacgtcg acgcccgcag tgacctgttt
     3421 agcctgggtt gcaccatgta ctacctgctg agcggccggg ttccgttccc gggtgatacc
     3481 gttgcggaat gcctcgctcg gaggcttcga ggcgggttgg tcccgatcac cgaactgaac
     3541 tccgagctgt ccccgcgact cgtggagttg cttgaaaagc tcttggcgcg ccgccccgag
     3601 gatcgtttcc agacggccgc tgaggccgcc cttgcgctcg aggccctggc tcatggtgag
     3661 gccgacgtcg caccgagtca tgaacttgcc cagcagccgg ccgcagacgc atttgtcgcc
     3721 gagcccgccc tatctggagg ggtggcgccc acgactcagc caccgtcttc ctccccaagc
     3781 gagtcgcc
//
LOCUS       DATAQL010001067         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4840574, whole genome shotgun sequence.
ACCESSION   DATAQL010001067 DATAQL010000000
VERSION     DATAQL010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4840574"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VHS97_18400"
     CDS             complement(<1..902)
                     /locus_tag="VHS97_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3450231.1"
                     /translation="MAHEEIPPSDVPKPVGHRDPSWRWGWLEPVGIVLLTLLLNLAGN
                     GRTGLWDRDEPRYAVCVREMRARGDWFFPTFNGEPRYHKPILIYWLMGLGTALGGDNP
                     FGVRLVSVAAGTASVLGVWLLGRKMLGKRGGRLAALILATAPIAAAESKLATTDATLA
                     LWLLGCQGCLWILGQRSSRTAAALFWIFLSLATLTKGPVGPALIAASSLVAWWWGWPS
                     LAWKRLHWRWGLAGFTIVTAPWFVVMSLATGGEFLRFAVGRQIIHRVASDMEAHGGFP
                     GYYPVVSLLAFYPWSALVPAALVGA"
     gene            1051..>1620
                     /locus_tag="VHS97_18405"
     CDS             1051..>1620
                     /locus_tag="VHS97_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450232.1"
                     /translation="MADNHPTEAACLLEQGASYHRRIGFKPVASPGNPIFAGFKTGAF
                     SLYFGDAPIYHFDLEGRWQRAFVDGTHYLKGLDATIQAIDRVREGPNLVLKRRNLSYA
                     EASDFDAMVRSTAIDLCTRIDAGRLDRIEPKSPKAGTLDADELRDFLDRISMWDSAAW
                     FAHRERYLAAYGPLPLLPPECQNAVVLQAT"
BASE COUNT          336 a          545 c          469 g          270 t
ORIGIN      
        1 gcaccgacaa gcgcggccgg taccaatgcc gaccacgggt agaaggccag gagtgaaacg
       61 accggatagt aacccggaaa gccgccgtgc gcctccatgt cgctggcgac gcgatggata
      121 atctgccgcc cgaccgcgaa acgcaaaaac tcgccgccgg tggcaagact catgacgacg
      181 aaccaggggg ccgtcacgat cgtgaagccg gctaggcccc accgccagtg cagccgcttc
      241 catgccaagc tcggccagcc ccaccaccag gccacgagcg acgaggcggc gatcaaggcc
      301 ggaccgacag ggcctttggt cagggttgcc aggcttaaga atatccagaa aagcgccgcc
      361 gccgtgcgcg acgaccgctg accgagaatc caaagacacc cttggcagcc cagcaaccag
      421 agtgcgagtg tcgcgtcggt cgtcgccagc ttcgactcgg cggccgcgat cggcgccgtc
      481 gccaagatca gcgcggccag gcggccgccg cgcttgccaa gcatttttcg gccgagcagc
      541 caaactccca gcacgctggc cgtgccggca gcgaccgaga ccagcctgac tccaaatgga
      601 ttatcaccgc ccaaagccgt tccaagcccc atcagccaat atatcaatat tggtttatga
      661 taacgaggtt cgccgttgaa cgtcggaaag aaccagtcgc cccgcgcacg catctcgcga
      721 acgcagacgg cataacgcgg ctcgtcacga tcccacagcc cggtccggcc gttgcccgcg
      781 aggttcagta gcaaggtcaa aagcacgatc ccgaccggct cgagccaacc ccaacgccac
      841 gacgggtcac gatgtccaac cggcttcggt acgtcgctcg gcggaatctc ctcgtgggcc
      901 acggctccat cctccttgcc cgcgcagagc gccctagcgg gtcaaccttc ctggctcgtc
      961 cgtcttggtt cgggcggcaa tcctgaggtt cgcatactat agtgaaagtt caacgtttcg
     1021 aggaggtcga tttcgaggac gacgagtctc atggccgaca atcatcccac cgaggccgcg
     1081 tgcctgctcg agcagggtgc atcgtaccat cgccgaattg gtttcaagcc cgttgcttcg
     1141 ccaggtaatc ccatctttgc cggcttcaaa accggcgcgt tttccctcta ttttggcgac
     1201 gccccgatct accactttga tctcgaaggg cgctggcagc gggcgttcgt cgatggcaca
     1261 cattacttga aggggctcga cgcgacgatt caggccatcg atcgagtgcg cgaaggcccc
     1321 aacctcgttt taaagcgacg caacctgtca tatgccgagg cgagtgactt cgacgccatg
     1381 gtgcgttcga ccgcaatcga cctttgcaca cgcatagacg cgggaaggct cgatcgaatc
     1441 gagcccaaat cgcccaaggc ggggacgctc gatgcggacg aactgcggga tttcctcgat
     1501 cgaatcagca tgtgggattc cgcggcctgg ttcgctcatc gcgagcggta tctagccgcg
     1561 tacggcccgc tgccactctt accccccgag tgccagaacg cggtcgtctt gcaagcgacc
//
LOCUS       DATAQL010001068         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_412839, whole genome shotgun sequence.
ACCESSION   DATAQL010001068 DATAQL010000000
VERSION     DATAQL010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412839"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VHS97_18410"
     CDS             <1..512
                     /locus_tag="VHS97_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3450233.1"
                     /translation="IRGKTLGVLGLGRVGSEVARIEGAFGMNLIAWSQNMTPEAAKAA
                     GAILVSKDQLFERADILTIHLVLSSRTRGLVGAAELERMKPTARLINASRGPIVDEQA
                     LISVLKNKQIADEAIDVFDIEPLPSSHPFRTLDNVLATPHIGYVSHGLYKTFYEDTVS
                     NIRKWLDTH"
     gene            629..796
                     /locus_tag="VHS97_18415"
     CDS             629..796
                     /locus_tag="VHS97_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3450234.1"
                     /translation="MTKKLERKTAVITGGTEGIGLATAKLFVQEGAYVFITGRRQKEL
                     DEAVKPLLLVH"
     gene            complement(829..>1665)
                     /locus_tag="VHS97_18420"
     CDS             complement(829..>1665)
                     /locus_tag="VHS97_18420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3450235.1"
                     /translation="FERYRLKGHSMESKTRRIIFAILPMMAVSYGTVKELMAQTNAPT
                     VVKNIVLVHGGFVDGSVWEGVYQALKKDGYTVAIVQNPTISLADDVAFTRRAIAAQNG
                     PVILVGHSYGGVVITEAGNDPKVAGLVYITAFAPDQGESVSSLIKDPPAGAPVPPILP
                     PQDGYLFLDKAKFPDAFAADLTPDVASFMADSQVPWGLDALNGAITRPAWRTKPSWYM
                     VSTEDRMIPPVAQRAMSKRAGSTVVEVKGSHAVYVSQPNAVARFIEEAATGSLGGTKG
                     KP"
BASE COUNT          360 a          475 c          480 g          350 t
ORIGIN      
        1 atatccgtgg aaagactctt ggcgttctgg gacttgggcg ggttggatca gaggtcgcgc
       61 ggatcgaggg cgcttttgga atgaatctga ttgcctggag ccagaatatg acgcccgagg
      121 ccgccaaagc agcgggtgca atcctggttt ccaaggacca gttgttcgag cgggccgaca
      181 tcctgacgat ccatttggtg ttgagcagcc gtacccgcgg tctggtcggg gcggcggagc
      241 tggagagaat gaaaccaacg gcccgattga tcaatgcgtc gcgcgggccg atcgtcgacg
      301 agcaagcttt gatcagcgtg ttgaagaaca agcaaatcgc cgacgaggcg attgacgtat
      361 tcgacattga gccgctgcct tcgtctcacc cgttccggac gctggacaac gtactggcaa
      421 caccgcatat cggctacgta tcgcatggtc tttacaagac gttctacgag gataccgtct
      481 cgaatattcg gaagtggctc gacacccatt aggcgccagc agaaccatct gtccgatcac
      541 atcggccagg cggtgctcgg acttaagtga tcgagagtga aggatcggaa gtcgcgagtt
      601 cagagctcag cagcaacgga gaaagacaat gaccaaaaag cttgaacgca agaccgcggt
      661 catcaccggc ggcacagaag ggattggcct agccacggca aaacttttcg tccaagaggg
      721 tgcctatgtc ttcatcacag gccgacggca gaaggaactc gacgaagccg ttaagcccct
      781 tttgctcgtt cactgattca ctcggaaatt atccgagcca gcctcctgtt atggttttcc
      841 tttagtgccg ccaaggctgc ccgtcgcagc ctcttcaatg aagcgagcga cagcgttcgg
      901 ctgcgacaca tatacggcgt ggctgccttt gacctcgacg actgttgagc cagcgcgttt
      961 ggacatggcc cgctgggcga cgggcggaat cattctgtct tcggtagaga ccatgtacca
     1021 gcttggcttt gtacgccacg ctggccgggt aatcgcgccg ttgagcgcat cgagacccca
     1081 cgggacctga gagtcggcca tgaatgaagc cacgtccggg gtcagatccg cggcgaatgc
     1141 gtccgggaac ttcgctttat cgaggaagag gtatccatcc tgaggcggca ggatcggcgg
     1201 aactggcgcg cccgccggtg gatccttgat gagcgacgac accgattcac cctggtccgg
     1261 cgcaaacgcc gttatgtaga ccaatccggc gaccttcgga tcgttgcccg cctccgtgat
     1321 cacgactcca ccatacgagt gaccgaccag gatcacgggg ccattctgcg cggcgatcgc
     1381 acgcctggtg aacgcaacgt cgtccgccag cgagatggtc ggattttgga cgatcgctac
     1441 cgtgtagccg tccttcttca gggcttggta aacgccctcc cagacagatc catccacgaa
     1501 cccgccatgc acaagtacga tgttctttac aactgtcggc gcgtttgttt gtgccataag
     1561 ctcctttacc gtgccataac tcactgccat catcggcaag attgcaaaaa tgattcgtcg
     1621 tgttttcgat tccattgagt gtcctttcaa cctgtacctc tcgaa
//
LOCUS       DATAQL010001069         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1939795, whole genome shotgun sequence.
ACCESSION   DATAQL010001069 DATAQL010000000
VERSION     DATAQL010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939795"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /gene="lnt"
                     /locus_tag="VHS97_18425"
     CDS             complement(<1..1601)
                     /gene="lnt"
                     /locus_tag="VHS97_18425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX3450236.1"
                     /translation="MPSLAPPDPLTHSLETASLSKRLAHHRVIAGIVSGVTLWMSFPP
                     VAWSWLAWIALVPFFWLVTLKGASWKTYLAAWCGGLVFWLLALEWVRMSDPSAWLGWL
                     LMALLFSLWWPGFIGLARWAVFRLQIPLLFAAPVVWVGLEYVRAYFLSGFPWYYLAHS
                     QFRQIYVIQIADLTGSLGISLLIAIVNAMVVDLVTLPLLHTTKTGTRLRPRQHVKLCL
                     VTILLGTTLCYGAFRVSTAAFRDGPKLALLQSNIEQRHKMKGDPAAIKTKLMGLVERA
                     LAHSERPDLIVWPETAYPYPYIAIDASVDPETLANQVGLISTKITPADWQKTQRLVAE
                     DLHDLTAKANVPMLIGSIYYHHRKSSREKYNSALLLRPGFTEVDFYHKMHLVPFGEYI
                     PFVETLPWLSILTPYHGQRVPSLSFGNDPRCLRLGDYVLAVTVCFEDTVPQVVARFFP
                     NDGKLGEPDVLINLSNDGWFHGSAELDMHLATGVFRAVEHRVPLARAVNTGLTALVDG
                     NGEIRAALPKESDGVLSVTVPLDDRG"
     gene            complement(1768..2997)
                     /gene="glp"
                     /locus_tag="VHS97_18430"
     CDS             complement(1768..2997)
                     /gene="glp"
                     /locus_tag="VHS97_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX3450237.1"
                     /translation="MLTLFEALDLVLSQVRALPPASCPVGEAVGSVLAEDVVADLDSP
                     PFDKALVDGFAIRTSDLAGPSRQLDVGESIMAGQTPSRALYQSEAAVVMTGAPGPPGC
                     DAVVMHERTERNGDRVLVLEPDVRAGQNLMARGREMRAGEVVVSSGSILRPAYLGVLA
                     SVGRTQVRVVPRPRVAIVSTGDELVEPGQTPGRGQIRNSNAVMLEALLKQDAAGVQVL
                     PIAPDEPGPLGQILGRGLDGDILVITGGVSAGERDLVPGSLQALGVSRIFHKVAVKPG
                     KPLWFGIGPPRGNDRPPALVFGLPGNPVSVLVGALLFVRSACRALAGNPSPRPAMSRV
                     RLNNRFAHRGDRMTFFPARLLEPDGSGAGRLSIETLAWSGSADLRAAAAADGFAVFAA
                     GDRDYAPGEIVDFLPMG"
     gene            complement(3019..3600)
                     /locus_tag="VHS97_18435"
     CDS             complement(3019..3600)
                     /locus_tag="VHS97_18435"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX3450238.1"
                     /translation="MILLIDNYDSFTYNLVQRLGEIDPGVDLRVVRNDQVTLEEIEAW
                     RPTHVIISPGPCTPVQAGVSNDIVRRFGPLVPLLGVCLGHQCIGHALGARIVRAGRIM
                     HGKTSLIHHDGRGVYRGLSNPFEATRYHSLLIEPGTLPAELEIVAWTDQNEIMGVRHK
                     RYALEGVQFHPESFLTDEGSKLLKNFMDQASHV"
     gene            3864..4352
                     /locus_tag="VHS97_18440"
     CDS             3864..4352
                     /locus_tag="VHS97_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450239.1"
                     /translation="MTATIVTVGLTLALNSFGLGHEPAHHHHHNSGSYILPPGPGDGW
                     GFPGDNPDKYGWTDYGVYLPLGADRTAEYYFPRYFAVPPEQMFITTYYNPFMTQGQQY
                     IPYCGAGGDHPMGGPPLASSELPVSPYAASPDTAPLVRVPRLNGRSEAAPIPSGGSGL
                     TP"
BASE COUNT          937 a         1357 c         1314 g          785 t
ORIGIN      
        1 cctcggtcgt cgagtgggac tgttaccgac aagacgccat cggattcctt tggcaaggcg
       61 gccctgatct cgccattgcc atccacgagg gcggttaatc cggtattgac ggctcgcgcc
      121 aggggaaccc ggtgttccac cgcccggaag actcctgtcg ctaggtgcat gtcgagctcc
      181 gccgaaccgt gaaaccagcc atcgttcgac aaattgatga gcacatccgg ctcgcctaac
      241 ttgccgtcgt tcgggaaaaa gcgggcgacc acctgcggaa ccgtatcctc gaagcaaaca
      301 gtgaccgcca ggacatagtc gcccagccgg aggcagcgtg gatcgttccc aaagctgaga
      361 ctgggcaccc gttggccgtg ataaggtgtc aggatcgaca gccacggcaa cgtttcgacg
      421 aaagggatat attctccgaa cggcaccaga tgcatcttgt ggtaaaagtc cacctctgtg
      481 aatccggggc gcaaaaggag cgccgaattg tatttctcgc gggatgattt gcgatgatga
      541 tagtagatgc ttccaatcag catcgggaca tttgccttgg ccgtcaggtc atggagatcc
      601 tcggcgacca gcctctgcgt tttctgccaa tccgccgggg tgatcttggt tgagatcaat
      661 ccaacctggt ttgcgagcgt ctctgggtcg accgatgcgt cgatggcgat gtatggataa
      721 gggtaggcgg tctccggcca gacaatcaag tcgggccgct cgctgtgagc gagcgctcgc
      781 tcaaccaacc ccatgagctt ggttttgatc gcggcgggat cacctttcat tttgtgcctt
      841 tgctcgatat tcgattgcaa aagcgcgagc ttgggcccgt cgcgaaatgc ggccgtggac
      901 acccgaaacg ctccgtagca cagcgttgtg cccaagagaa tggtgaccag gcagagcttg
      961 acatgctgcc gcggacgcag ccgcgtaccc gttttcgtcg tgtgcaagag cggcaaagtc
     1021 acgagatcga caaccatcgc gttgacgatc gcgatcagca gactgattcc cagtgagccc
     1081 gtgagatcag cgatctgaat cacgtagatc tggcgaaact ggctatgggc caggtagtac
     1141 cagggaaagc cactcaagaa gtaggcgcgg acgtattcca gaccgaccca gactactgga
     1201 gcagcgaaga gaagggggat ctgcaggcgg aataccgccc accgcgccag gccgataaac
     1261 ccgggccacc agagcgaaaa caagagcgcc atcaatagcc aaccgagcca agcgctgggg
     1321 tctgacatgc gcacccattc cagggcgagc agccagaaaa ccagacctcc gcaccaggcc
     1381 gccaggtacg tcttccatga tgcgcccttc agagttacga gccagaaaaa gggcacgagg
     1441 gcgatccagg ccagccaact ccatgcgacg ggcggaaacg acatccagag agtcacgccg
     1501 gagacgatcc ccgcgatgac acgatgatgc gcaaggcgtt tggacaggct ggccgtttca
     1561 agcgagtggg taagggggtc gggcggtgca agcgaaggca tcgaggttac cttgtaagaa
     1621 agcgatcctc cgcaagcaga ctgtccgccc ggcgagtaga tcttgccgca ccgcgaccca
     1681 cggcgtggca gggggagaga cgggcttcac accccaggcc ggaagaaatc agcgacgcgt
     1741 atcgaggctc gtccttcgcg gctcatgtta tcccatggga aggaagtcga caatttcacc
     1801 tggcgcgtaa tcccgatcgc cggcggcgaa aacggcgaag ccgtcggcag ctgccgcggc
     1861 ccgcaaatcg gccgagcccg accatgcgag agtttcgatg gacagccggc cggccccgct
     1921 gccatctggt tcgagcaacc tggcggggaa aaaggtcatt cggtcgccgc gatgcgcgaa
     1981 tcggttgtta agtcggaccc gggacatcgc tggtcgcgga ctcgggttgc cggcaagagc
     2041 ccggcaggcc gatctgacga acaacagagc gccgacaagc acgctcacgg gattgcccgg
     2101 caacccgaac acgagtgcgg gcgggcggtc attcccccgc ggggggccga tcccaaacca
     2161 gaggggtttg cccggcttca cggcgacctt atgaaaaatg cggctgaccc ccaaggcctg
     2221 cagagacccc ggaacgagat cgcgctcgcc ggccgagacg ccgccggtaa tgaccagaat
     2281 atccccatcg agtcctcgac cgaggatctg gccaagcggc cctggttcgt cgggcgctat
     2341 cggcagcacc tggacgccgg ctgcgtcctg tttcaagagc gcctcgagca tcacggcgtt
     2401 cgagttgcgg atctgcccgc gtcccggcgt ctgcccgggc tcgaccaact cgtctccggt
     2461 cgacacgatt gcgactcgtg gtcgaggtac gacccgtacc tgcgtgcgac cgaccgaggc
     2521 caggactccc aggtacgccg gtcgaaggat cgatccactc gagaccacca cttcgccggc
     2581 gcgcatctcc cgaccgcgcg ccatcaggtt ttgtcccgca cgaacgtcag gttctaaaac
     2641 aagcacgcga tcaccgttgc gttcggtccg ctcgtgcatc acgaccgcat cgcaacccgg
     2701 cggaccggga gcgcccgtca ttacaacggc agcctcgctc tggtagagcg cccgagacgg
     2761 ggtctgaccg gccatgatcg attcacccac gtcaagttgg cggcttggac cagccaggtc
     2821 cgacgtcctt atggcgaatc catcgacgag cgccttgtcg aacggcggag aatcgaggtc
     2881 agcgacgacg tcttcggcca gcacagaacc cacggcttcg cctaccgggc aggacgccgg
     2941 cggcaacgcc cgcacctgac tcagaaccag atcgagagcc tcgaacaatg tcagcatggc
     3001 atcactctgc acactgttct agacatgact cgcttgatcc atgaagtttt tgaggagctt
     3061 tgatccttca tctgtcagaa aactctccgg atgaaactga actccttcca aggcgtagcg
     3121 tttgtgcctc acgcccatga tctcgttttg atcagtccag gccacgatct cgagctccgc
     3181 cggcagcgtc cccggctcga tcaacaggct gtgatatcgg gtcgcctcaa acggattgga
     3241 aagaccacga tacactccgc ggccgtcatg atgtatcaac gacgtcttgc cgtgcatgat
     3301 ccggcccgcg cggacgatcc gcgcgcccaa ggcatgaccg atgcactgat ggcccaggca
     3361 gactcccaaa agcggcacca ggggcccaaa tcgcctgacg atgtcgttcg acactccagc
     3421 ctgaacaggt gtacaaggac cgggcgagat gatcacgtgc gtgggtcgcc aagcttcgat
     3481 ttcttcgagc gtcacctggt cgttacgaac cacccgaagg tccacgcccg gatcgatctc
     3541 gccgagccgt tgaacgaggt tgtatgtaaa cgaatcgtag ttatcgatca ggaggatcat
     3601 gcgttatgcg ccgatcacat ggtggccaag tcgcgaaata gagcgcaggg attttccgcg
     3661 ttcttggatc gtaacgccca gcggatcggg ccggaagaga aacctgggcc aatcttccac
     3721 gtgggtaact ggtgagtgat atagttcgcg atgacggtca tggtgctggc ggccagcgaa
     3781 tcaagcgcgt ggcgatcccg ccgcgaacta gattggcaaa ctacttaccg cgcggcctgt
     3841 ccgaccgaac acggaggatt gcaatgacgg ctacgattgt cacggtcgga ttgaccctcg
     3901 ccttgaactc gttcgggctg ggtcatgagc cggcccatca tcatcatcac aattccggaa
     3961 gctacatctt acccccaggt ccgggcgacg gctggggatt tcccggcgat aatccggata
     4021 agtacggctg gaccgattac ggcgtctacc tgccattagg cgcagaccgc accgcggaat
     4081 actatttccc gcgctacttc gccgttcctc ccgaacaaat gttcataacg acgtactata
     4141 atcctttcat gacgcagggc cagcagtata ttccctattg cggcgccggc ggcgaccatc
     4201 cgatgggcgg gccgcccctg gcttcttcgg agttgccggt ctcaccctat gccgcctcgc
     4261 ccgacaccgc cccgctggtt cgagttccgc gcttgaacgg cagatccgag gccgcgccta
     4321 ttccctcggg cggttccgga ttgactccat gacggccggt acgacggggg agcgaataag
     4381 gtggcacaag aaa
//
LOCUS       DATAQL010001070         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5300043, whole genome shotgun sequence.
ACCESSION   DATAQL010001070 DATAQL010000000
VERSION     DATAQL010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5300043"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VHS97_18445"
     CDS             <1..290
                     /locus_tag="VHS97_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450240.1"
                     /translation="IPKKVKVPDYAHLLPEPIRRFTQPAAIQDAEHLSFLQGGGHGGS
                     HPHLVHAFLSAVKGDRPPLPDAATSANWTMVGLCAHESALKGGEKVVRPTF"
     gene            complement(274..>3294)
                     /locus_tag="VHS97_18450"
     CDS             complement(274..>3294)
                     /locus_tag="VHS97_18450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3450241.1"
                     /translation="GISPEVKDRGGDPDLSQGQCEAETVQGPGALDTKLWEQESLAPE
                     ELVARLCDDQVRRWNLGQRVPAEAYLAQCPRLDPDGEAAFELIYGEFLLREHLGDAPT
                     TEEFSWRFPWFAARFARQLELHGILNSEVSGPQTIVRSGARGIAGEKKTGGPNRSVAP
                     GYEILGELGRGGMGIVYKAWQVSLKRVVALKVIRAGAHTDADAAARFHAEAEASARFQ
                     HPNIVQVFEVGENEESAYLVLEYVSGGGLDRKLSGSLQDPGVSAKLIESLARAIHYAH
                     RRGIVHRDLKPANVLLTEDGIPKITDFGLAKLLERDDGLTNVGELLGTPSYMAPEQVK
                     GTSRQITPATDVYALGAILYEMLTGRPPFKGTTPLSTLEQVSSQEPLTPSKIQRHTPR
                     DLETICLKCLQKDPRRRYATADLLADDLRRFLDRQPILARPTPAWERAWKWALRRPAA
                     AAVLVSVLAAVILFLVMGWHYNTRLRAALRTAQAAEQEADSSAGRAVEQRNRALQAFK
                     KLVYDVQEKLGQTPATRSIRQSLLNTAITGLEEIASSTAGSPPDLSQAVAHQKLGDIF
                     RIIGRSAEARRHYDQSRSLAEALLASDPDSLPITEDLYQTHMGLGSLHVTAGQLDAAK
                     VELARAVAMAEAIAAHPKNDAKPQGLIEAYLQLGRAYSFAREYDSAEKWFRKMQDLAA
                     RWVADEPADVQARDFLAASYRKLGDLKKFAKDFAAAREGYVRAIAIGRQVLLDEPGSL
                     PFKAHLGLGLDDLAGVARSQGKIDEARKLFQEAEQLYSELVESDPELRESRFTLLHTR
                     FNQAKMEEGELRFSRAAELWRRIRDQLRQLQRDGLLEGRTDHSTNPSVVAGELAYCEA
                     APTAIKDLDFARSRPALVSARLLLLHARMFDQKTNDQNRLAAVGEALTGLTSTAPQEL
                     LELARYLAIFIKDLDSGRWPGFREAERQQLRARCTSRGTELLRLAAERGFDDRALLES
                     AELAPLRRQPGPRPLAADTKSTASAPAGRVKTSA"
BASE COUNT          586 a         1033 c         1031 g          644 t
ORIGIN      
        1 agatccccaa gaaagtgaaa gtgcccgatt atgcgcatct tctccccgaa ccgattcgcc
       61 ggttcaccca acccgcggcg atccaggacg ccgagcacct ttccttcttg caaggaggag
      121 gccatggcgg gtcgcatccg cacctggtcc atgccttttt gagcgcagtc aagggggatc
      181 ggccgccttt gcccgacgcg gcgacctcgg ccaactggac catggtggga ttatgtgccc
      241 acgaatcggc gctgaagggg ggcgaaaagg ttgtcaggcc gacgttttga cgcggccggc
      301 cggggcgctc gccgttgatt tggtgtccgc tgcgagcggc ctgggcccgg gctgcctgcg
      361 cagcggcgcc agctcggcac tctcgaggag tgcgcggtcg tcgaaaccgc gttccgcggc
      421 tagacgcaac aattcggtac cgcggctcgt acagcgtgcg cgcagctgct gccgttctgc
      481 ctcacggaag ccaggccaac gccccgaatc gagatcctta ataaagatgg ccaggtaccg
      541 cgccagttca agcagttctt gcggagcggt cgatgtcagg ccggtcagag cctcacccac
      601 tgccgcgagc cggttctggt cgtttgtttt ctgatcgaac attctggcgt gcaagagcag
      661 caaccgggcc gatacgaggg ccggccggga acgggcgaaa tcaagatctt tgattgccgt
      721 gggggcagcc tcgcaatagg cgagttcgcc cgccacgacg ctgggattgg tggagtggtc
      781 ggttcgccct tccagaagac cgtcacgctg tagctgacgc aactgatcgc gaatgcggcg
      841 ccagagctcg gccgccctgc taaaccggag ctcgccttcc tccatcttgg cctgattgaa
      901 ccgggtgtgc aggagagtga accgagattc gcgaagctct gggtctgact cgacgagctc
      961 ggaatagagc tgctcggctt cttggaagag ttttcgcgct tcgtcgatct tgccctggga
     1021 tcgggcgact ccggcgagat cgtcgaggcc gagcccgagg tgcgccttga agggcaaact
     1081 gcccggctcg tccaggagaa cctggcggcc gatagcaatc gccctgacgt agccttctcg
     1141 agcggccgca aagtcctttg caaacttttt gagatcgccc agcttgcggt agctcgctgc
     1201 cagaaagtct ctggcctgaa cgtcagccgg ctcatccgcc acccagcgcg cggccaggtc
     1261 ttgcatcttg cgaaaccatt tttccgctga gtcatattcg cgcgcgaagc tgtaggctct
     1321 gccgagctgg agataggctt cgatcaagcc ctgcggcttt gcatcatttt tgggatgggc
     1381 cgcgatcgct tccgccatgg cgaccgcgcg ggcgagttcg accttggccg cgtcgagctg
     1441 cccggccgtc acatgcaatg atcccagccc catatgcgtt tggtaaaggt cttcagttat
     1501 aggaaggctg tcgggatccg acgcaaggag agcctccgca aggctgcgag actggtcgta
     1561 atgccggcgt gcctcggccg accggccgat gatccgaaag atgtcaccca gtttctggtg
     1621 ggcgaccgcc tggctcaggt cggggggtga acccgccgtg ctgctggcga tctcttcgag
     1681 acccgtgatt gccgtgttca agagcgactg gcgtatcgac cgggtcgccg gcgtctggcc
     1741 cagtttctcc tggacgtcgt acaccagttt tttgaaggcc tgcagtgccc ggtttcgttg
     1801 ctcaactgcc ctgccggcgc tggaatcggc ttcctgctcg gctgcttgtg ccgtccgaag
     1861 ggccgcgcgc aggcgggtgt tgtagtgcca tcccataacc aggaagagaa tcacggcagc
     1921 aagaacggac accaagaccg ctgcagccgc cggtcggcgc agagcccatt tccaagcgcg
     1981 ctcccaggcg ggtgtcggcc gggccaggat tggctgacgg tccagaaatc gacgcagatc
     2041 gtccgccagc aggtcggccg tggcataccg ccgtcgcggg tctttttgga ggcacttgag
     2101 acatattgtc tccaggtcgc ggggcgtatg ccgctggatc ttgctgggag ttagcggctc
     2161 ctggctcgag acctgctcca gggtcgatag aggcgtcgtt cccttgaacg gcggccggcc
     2221 cgtcagcatc tcataaagaa tcgcccctaa agcgtaaaca tcggtcgccg gcgtgatctg
     2281 gcgcgacgtt cccttgacct gctcgggagc catgtagctg ggcgtgccca agagctcgcc
     2341 gacattggtc agaccgtcgt cccgttccag gagcttggcg agtccaaagt cggtgatctt
     2401 gggaattccg tcttcggtca agagcacgtt ggccggtttc aagtcgcggt ggacgattcc
     2461 ccgccggtga gcatagtgaa tggcccgagc aaggctttcg atcaacttcg ccgacacgcc
     2521 tggatcctgg agcgagcccg aaagtttgcg gtcgaggccg ccgcctgaaa catattcgag
     2581 gacgaggtag gccgattctt cattctcgcc gacctcgaaa acctgaacga tgttaggatg
     2641 ttgaaagcgg gcggacgcct cggcctcggc gtgaaaccga gcggccgcgt cggcgtctgt
     2701 atgggcaccg gcacgaatca ccttcaaagc cacgactctc ttcaagctga cttgccacgc
     2761 cttgtatacg attcccatgc cgccacgccc gagctcgcct aagatctcat aaccgggagc
     2821 gaccgatcga ttcgggccac ccgtcttctt ttcgccggct atgccgcgcg cgccggaccg
     2881 tacgatcgtt tggggacccg aaacctctga attgagtatg ccgtggagct caagttgcct
     2941 cgcaaagcga gccgcaaacc aagggaatcg ccaggaaaat tcctccgttg tcggtgcgtc
     3001 gcccagatgc tcacgcagga gaaactcgcc gtagatcagc tcgaaggcgg cctcgccgtc
     3061 ggggtctagc cgaggacact gcgccagata ggcctcggcc ggcacccgct gaccaagatt
     3121 ccagcgccgc acctggtcgt cgcacagccg cgcgaccagt tcctcggggg ccagcgattc
     3181 ctgctcccac aacttcgtat ccagtgcacc gggaccctgc acggtttcgg cctcgcactg
     3241 cccttgcgat aaatcggggt cgcccccgcg atccttgact tcagggctta tgcc
//
LOCUS       DATAQL010001071         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4535409, whole genome shotgun sequence.
ACCESSION   DATAQL010001071 DATAQL010000000
VERSION     DATAQL010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4535409"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..717
                     /locus_tag="VHS97_18455"
     CDS             31..717
                     /locus_tag="VHS97_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3450242.1"
                     /translation="MRILIVEDEADLADFVVRGLREDGFTVEHALDSAQGTEALESGV
                     WDLIVLDWGLPGEDGISFLRRFRHAGGTTPVLFLTARDSVADRVRGLDIGADDYLCKP
                     FAFDELLARVRALVRRRDRVPDIWINYGDVSIDIAKQRALRAGHTLDLTAKELALLTF
                     FLRHPAQVLSRTRIYDHVWDENFDLLSNTLEVHIMELRRKLEAHGPRLIQTIRGRGYL
                     FDVAPGSEAK"
     gene            714..2276
                     /locus_tag="VHS97_18460"
     CDS             714..2276
                     /locus_tag="VHS97_18460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450243.1"
                     /translation="MSLTGRFSLFFLSALGLVLLGFSLTLYVSARVYLGRQLSERLAA
                     SLAVLAAAAEVHPEGVEWEPQERVLPLGQESGPERLRWMVFDPAGRRIDHSHNLVDAE
                     LTAAWNPGPGKTGLAGRLADGKGRLWKLSQRAISSGLARPFRSTAETDSTRTSDLPSA
                     EAFYPFLVLTVCAPLDPMQSTLAALAWFLVTLSVSIWLLSALLCRWLSRQALIPLTKL
                     VESARNLDATDPGWCLEQVGTGDELDLLGRTFNDLLSRLHLAYERQRRFSSDASHQLR
                     TPLTVLIGQIEVALRQERPLEEYRRVLKSALGRAGQLARIVESLLFLARAETHAALPE
                     CVCLELNHWAALHLANRTATDPAIEIAHVGAQSEGLCVRAHASLLDQLLDNLLDNAGK
                     YSPPGARVVVETVRDGENAVLAVEDHGPGIAPADQARVFEPFFRAAGGGVPGVGLGLA
                     VVHRIAIAFGGSVTVRSELGKGARFEIRLPIADARFSLLDDSKPEDLSKAVLAHRHGA
                     AAAIFDTGNLTG"
BASE COUNT          426 a          749 c          770 g          541 t
ORIGIN      
        1 agggacgatc gagcaactgg accaaaggca atgcgcatcc tcatcgtgga agacgaagcc
       61 gatcttgcgg attttgtcgt tcggggcctg cgcgaggatg gattcacagt cgagcacgca
      121 cttgatagcg cgcagggtac cgaagcgctc gaatccggtg tttgggactt gatcgtgctg
      181 gattgggggc tccccggtga ggatggcatc tcctttttgc ggcgatttcg gcacgcaggc
      241 gggacgacac ccgtgctgtt cttgacggca cgcgactcgg tcgccgaccg agttcggggt
      301 ctcgacatcg gagcggacga ctatctctgc aaacccttcg cttttgacga actgctagcg
      361 cgcgtccggg ccctggtacg gcgacgagat cgagtgccgg atatctggat caactacggc
      421 gacgtgagca tcgacattgc caaacaacgg gcactgcgcg cgggtcatac actcgacctg
      481 accgcgaagg aacttgcgct cttgacgttc tttttgcggc acccggctca ggtgctatcg
      541 cggactcgca tttacgacca tgtctgggac gaaaactttg acctgctctc caatacactg
      601 gaggtgcaca tcatggagct gcgtcgcaag ctggaagccc acggcccgcg gttgatccag
      661 acaatccgag gtcgcgggta tctcttcgac gttgctcccg gatcggaggc gaaatgagcc
      721 tgaccggccg tttcagtttg ttttttctgt cggccctcgg actggtcttg ctcggttttt
      781 cgctcaccct ttacgtgtcg gcgcgggtct atctgggtcg tcagctcagc gagcgtctag
      841 ccgcttcgct ggccgtgctg gcggcggcgg ccgaggttca ccccgagggg gtggagtggg
      901 aaccgcagga acgtgttctc ccactcggcc aggaatccgg acccgagcgg ctgcggtgga
      961 tggtcttcga cccagcgggc cgtcgtatcg atcattcgca taaccttgtc gacgccgagc
     1021 ttaccgccgc ctggaacccg ggcccgggca agacggggct cgcaggccga ttggccgatg
     1081 gcaagggccg gctctggaaa ctatcacagc gcgcgatctc atcggggttg gcccggccgt
     1141 ttagatcaac ggccgaaaca gattcaaccc gaacgagcga tttgccttcc gccgaagcgt
     1201 tctatccatt tctggtgctc accgtttgcg cgcctctcga cccgatgcag tcaacgctcg
     1261 ccgcgctcgc ctggttcctc gtgacgctga gcgtgtcgat ctggcttcta tctgcattgc
     1321 tttgccggtg gctatcacgc caggcgttga tcccgctcac aaagctggtc gagtcggctc
     1381 gaaatctcga tgcgaccgat cccggatggt gtttggagca ggtcggaact ggtgacgagc
     1441 tcgatcttct ggggcgtacc ttcaatgatc tgctgtcgcg gctgcacctc gcctacgagc
     1501 gccagcggcg tttcagcagt gatgcctcgc atcaattgcg caccccgctc accgtgctga
     1561 tcggccaaat cgaagtcgcc ctccggcagg aacggccgct cgaagagtat cgacgcgttc
     1621 tcaaatccgc gcttggtcgg gctggtcagc tcgcccggat cgtcgagtcg ctcctctttc
     1681 tggccagggc cgagacccat gctgcgcttc ctgagtgtgt gtgtttggaa ttgaatcatt
     1741 gggccgcgct tcaccttgcc aatcgaaccg cgaccgatcc ggcaatagag atcgctcatg
     1801 tcggcgcaca aagcgagggc ctgtgcgtaa gggctcatgc cagccttctc gaccagttgc
     1861 ttgacaactt gctcgacaat gccggcaagt acagcccgcc gggggcgcgg gtggtcgtcg
     1921 aaacggtccg tgacggcgag aatgctgtcc tcgcggtcga ggatcacgga ccaggcattg
     1981 caccggccga ccaggcccgt gtttttgagc ccttttttcg cgcggccggc ggaggtgtgc
     2041 cgggcgtggg actgggactg gcagtcgtcc atcgaatcgc gatcgctttt ggcggttctg
     2101 tcaccgtgcg cagcgagctc ggcaaaggcg ctcggttcga gattcgtcta ccgatcgctg
     2161 acgcgagatt ctctctgctc gacgattcga aaccagagga cctgtcgaaa gcggtattgg
     2221 cccatcgcca cggggctgcc gccgcgatat tcgacaccgg caatctgaca ggttgatgtt
     2281 gaccggaacg tcgtcgtcgg gaagatgtgg cacttcgccg tcccattcgt taggtccgta
     2341 agagtctttc cattcggaga actgcggtcg gtttatcgcg ttttcccaaa ctaacgaccc
     2401 tgggtcgccg gctgtcccca ctcacactca atcagggcac gaactgccct ggggtagggt
     2461 tctttgtcgc attttgctct aggact
//
LOCUS       DATAQL010001072         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5451925, whole genome shotgun sequence.
ACCESSION   DATAQL010001072 DATAQL010000000
VERSION     DATAQL010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5451925"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..946
                     /locus_tag="VHS97_18465"
     CDS             275..946
                     /locus_tag="VHS97_18465"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX3450244.1"
                     /translation="MKLLRDPLGRPIPRLYFKVDELDRECEQIVTNFMDRHSAGFSLP
                     VPTDDIIRMIEMEADDFDMYADLPEELDGYTDFFFDRKPRVRIAKRLSDPRYENRLRM
                     TLGHEFGHVRFHAPLWRDGHVERDRRPAEPCWTCNRDTIVAAPENDWMEWQAAYIGGA
                     LLMPREPVRLLVRGIGVPQAPETAVGADSDLGQAAILRVTKGCQVSQQAARVRLAKLG
                     LLSQT"
     gene            984..1412
                     /locus_tag="VHS97_18470"
     CDS             984..1412
                     /locus_tag="VHS97_18470"
                     /inference="COORDINATES: protein motif:HMM:NF044909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450245.1"
                     /translation="MNEIERSLESFYRDYIDRFNTADVERFLEYFARPYVSISGERGV
                     RVIPNDAGHVSGFRRVMDGLKERGWVRSEIVAIKAWALDDNLGMILSDVVRVRADNSV
                     LEEVRACYLVRRENQAWKIATISEVRPPHLGPGNVPRPSP"
     gene            1591..1953
                     /locus_tag="VHS97_18475"
     CDS             1591..1953
                     /locus_tag="VHS97_18475"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3450246.1"
                     /translation="MTYLCLVYFEPERMEGLSRSEKIALDRNSVAYDGELSRSGHYIV
                     SHALQPVSTAKTVRVRRGKASQVDGPFAETKELLGGFILINAANMNEAVEIAAKIPMA
                     EMGSIEVRPVMKIDPEAH"
     gene            complement(2317..>2499)
                     /locus_tag="VHS97_18480"
     CDS             complement(2317..>2499)
                     /locus_tag="VHS97_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450247.1"
                     /translation="LGIGLYRGPQSTCFWHWGDNLDFQSYLIGCAHEKIGAVVMTNSS
                     RGLGPARKIAARAIEG"
BASE COUNT          530 a          712 c          761 g          496 t
ORIGIN      
        1 tggtaatccc ataataaacg ctgcgcttaa tatgggccag acttagagcc ggtattaaac
       61 gcgacgcgac gtaaagaaga ctggcgttct gttaatcagc aattgccgcc cgaggatttt
      121 taccgagcat ctcgcgagga ttgtcggctg ctggcccgat gtgctgtatt ttcagcccaa
      181 ctgccattcc cccgacgcta aggccgaggc tggccaagat cgaggccgtc taccgcgtcc
      241 gccgaagctc tgggggttag tgtgggaggc ggaaatgaag ttgcttcgcg atccgctcgg
      301 acgcccgatt ccccgccttt acttcaaagt cgacgagctc gaccgggagt gcgagcagat
      361 cgttacaaac ttcatggatc ggcactcggc cggcttcagc cttcccgttc ccaccgacga
      421 catcattcgc atgatcgaga tggaagcaga cgacttcgac atgtatgcgg acctgcctga
      481 ggagttggac gggtacacgg atttcttttt cgatcgaaaa ccccgcgtcc gaatcgcgaa
      541 acggctttcg gatcctagat acgaaaatcg cctccgtatg accctcggcc atgagttcgg
      601 acacgtccgc tttcacgctc cactatggag agacggccat gtcgagcgcg accgccggcc
      661 ggctgagccg tgctggacct gcaaccggga caccatcgtc gcggcacccg agaacgactg
      721 gatggagtgg caagcggcat acattggtgg cgcgctcttg atgcctcgcg agcccgtcag
      781 gctcctggtg agagggatcg gggtccccca ggcaccagag acggcggttg gtgccgattc
      841 agatttgggc caagccgcga tcctgcgggt gacgaaaggc tgccaggtct cccaacaggc
      901 cgcgagggtg cggcttgcga agctggggtt gctgagccaa acctgagaat tgcctcacct
      961 cgagaccgta gaggaacgag atcgtgaacg agattgaacg cagtctggag agcttctaca
     1021 gagactatat cgatcggttt aacaccgcgg acgtcgagcg tttcctggag tactttgcac
     1081 gcccgtacgt atcgatcagc ggtgagcgcg gagtccgggt gattccgaat gacgccggcc
     1141 atgtgagcgg ttttcgccgt gtcatggacg ggctcaagga gcggggatgg gtgcgctcag
     1201 agatcgtcgc gatcaaggct tgggcgctcg acgacaatct aggcatgatc ttgtccgacg
     1261 tagtgcgggt gagggccgat aattcggtcc tcgaggaagt ccgcgcctgc tacctggtcc
     1321 gccgcgaaaa ccaggcatgg aaaatcgcca ccattagcga ggttagacct ccacatctcg
     1381 gccccgggaa tgttccaagg cccagcccgt aaagagttac gtcccgtctt gattcggccc
     1441 ttttgcgtgt agcggcggat cgtcctcgcc atcacttgaa aattagcttg cgggagtcgt
     1501 caggaacctc cttttccaaa tgtcgattcg gcgcttaatc aggcgactaa cagatgccga
     1561 actcggcaat cactaaatca ggagtgaacg atgacgtatc tatgcctggt ctatttcgag
     1621 cccgagagga tggaagggct ctcgcgcagc gaaaagatcg ccttggaccg caactcggtg
     1681 gcctacgatg gcgaacttag ccgaagcgga cactacatcg tgtcccacgc cctccagccg
     1741 gtgagcaccg ccaaaacggt ccgcgtccgg cggggcaagg cttcccaagt cgatggccca
     1801 ttcgccgaga ccaaagaact tctaggcggc ttcatcctga taaatgcggc aaacatgaac
     1861 gaagcggttg agatagcggc gaagatcccg atggcggaga tgggcagcat cgaggtgcgg
     1921 cccgtgatga aaatcgatcc ggaggcgcac tgattctgct ccccatattc ctcggggcga
     1981 cggacacctc aaagaggaac cggcaggtgg aagccagcgg tgggcacctg ccggggttcc
     2041 tcaagggagc acttgagcag cggtccctgt gtcgatcgtc gcaaagacgc gatcgtagtc
     2101 ggcgctcccg ttccggaggg caagcctgat aggcccggtc gagaaaaaga ccgttgtggc
     2161 cgatcgtcga ggtcgcagtc cgcggcaact ggaaacgcgg cttgaattgc acatctctcg
     2221 gtccgatcga gcgcaagtct tccccggtgc gcgactgctt gatgttttgg aaggtccgtt
     2281 catcgtgtca tcgagcgata acgaacgatg atcgcgtcaa ccttcgatcg ctcgggcggc
     2341 gatttttctt gctgggccaa gtccgcgaga gctgttggtc ataaccacgg cgccgatttt
     2401 ctcgtgcgca catcctatca ggtagctctg aaagtccagg ttgtcgcccc agtgccagaa
     2461 gcaagtcgac tgtggaccgc ggtaaaggcc gatccccaa
//
LOCUS       DATAQL010001073         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1178336, whole genome shotgun sequence.
ACCESSION   DATAQL010001073 DATAQL010000000
VERSION     DATAQL010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178336"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2462
                     /locus_tag="VHS97_18485"
     CDS             <1..>2462
                     /locus_tag="VHS97_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3450248.1"
                     /translation="PGPGRDRVKVIEDSDGDGKADRFTVFADGLNIPSGIAVGHGGVW
                     VANSPDILFLEDRDGDGKADFRQVVATGFGRSDTHELPNSLTWGPDGWLYGWNGVFNP
                     CKVASKNGKTYEFTCAIFRIHPTTRVFEVWCEGTSNPWGIAIDPEGSFFASACVIDHL
                     WHLVETGYYIRQGGPYPPFTWPIESIVGHLHQKAAYCGAHYFDSPAYPPEYRGRLYMG
                     NIHGNGVNVDVLDRNGSTYIGKPAPDFLRANDSWFMPVAQKTGPDGCLYVLDWYDRYH
                     CYQDANRDPAGIDRLKGRLYRIRYNDTPRRAGFDLAKASDDQLVELLGSSNVYDRDIA
                     QRLLTERGTESVRGKVEAVVLNQNVARRARMHGLWALVGMGMLEPGFHSKLLAHGDPT
                     FRAWGVRAAGNMRRVDSRIRDDVIKLSRDPSPDVRLQVAIASKKIEGVDPLTVSLDVL
                     RFGHGDPLIPHIVWQNLQSIVEDRQDELARWLEPFRGKEIGFSPLVPHVIERLLASPN
                     ADARIAARLLYSCTEEDAAREALDIMAEAFRERRMPAELNDGLRKELSIVLHELTGQR
                     GDPLATDYAVLAAYCGDRESLDQARRIAQSNSFDGDARLRALAAIMFLERPSSIHPIV
                     EKILTERDSPDSTGFRGKVLDSLGRLDDPALAPIVLAALASLPPELKPRVIELLTERS
                     SWSKALLGAIEAKQIPSSALNINQLRRLQKNKDPEITDRVTKIWGTIRQGRNPDRERL
                     VAGMKNLIRTTAGDPVAGQAVFNKLCSQCHKIYGEGQDVGPDITANGRNDFDQLLSNV
                     FDPSLVIGPGYQATTVATTEGR"
BASE COUNT          490 a          749 c          754 g          469 t
ORIGIN      
        1 accgggaccg ggccgcgatc gggtcaaggt gatcgaggat tccgacggtg acggcaaggc
       61 cgaccggttt acagtctttg ccgacggtct caatatccca tcgggaatcg cggtcggcca
      121 cggcggcgtt tgggtggcca attcgcccga catcctgttc ttggaagatc gcgacggcga
      181 cggcaaggcc gacttccgcc aagtggtcgc gaccggcttc ggccgcagcg atacccacga
      241 gctacccaat tcgctcacct ggggtccgga cggctggctc tatggctgga acggcgtctt
      301 caacccctgc aaagtcgcct ccaagaacgg caagacctat gagttcacct gcgcgatctt
      361 tcggattcat cccacgacgc gggtgttcga agtctggtgc gaaggaacaa gcaatccctg
      421 gggcattgcg atcgaccccg aaggctcgtt tttcgccagc gcttgcgtga ttgaccacct
      481 ctggcatctc gtcgagacgg gctattacat tcgccaggga ggaccgtatc ccccatttac
      541 ctggccgatc gagtcgatcg tcgggcacct gcaccagaag gccgcttact gcggcgctca
      601 ttacttcgac agcccggcct atcctcccga gtatcgcggc cgattgtaca tgggcaatat
      661 tcatggcaac ggcgtgaacg tcgatgtctt ggatcggaac ggctcgacgt atatcggcaa
      721 gcccgcgccc gactttctcc gcgccaacga ctcctggttc atgccggtgg cgcaaaagac
      781 cggacccgat ggctgcctgt atgtcctcga ctggtacgac cgctatcact gctatcaaga
      841 cgccaaccgc gatccggcgg ggattgatcg gctcaaggga cgtctctaca ggatcagata
      901 caacgacacg ccgcggcgag ccggtttcga cctcgccaag gcgagcgacg atcagctggt
      961 cgagctcttg ggcagctcca acgtctacga tcgagatatc gcccagcggc tgttgacgga
     1021 acgaggaacg gagtccgttc gtggcaaggt cgaagccgtt gttttgaacc aaaatgtcgc
     1081 gcgcagggcg cggatgcatg gactctgggc attggtcggg atgggaatgc tcgagcctgg
     1141 ttttcattcc aagcttttgg cccacggcga tccgaccttc cgagcatggg gcgtacgcgc
     1201 ggcggggaac atgcgaaggg tcgatagccg gatccgtgat gacgtgatca aactcagccg
     1261 cgaccccagc ccggacgtgc ggctgcaggt ggcgatcgcg tccaaaaaga tcgagggggt
     1321 cgatccgctg actgtatcac tcgacgtcct tcggtttggt catggtgatc cgctcattcc
     1381 tcacatcgtc tggcaaaacc tgcagtcgat cgtggaagac cggcaggacg agctcgctcg
     1441 ctggctggag ccattccggg gcaaggaaat cgggtttagc ccgttggttc cccacgtgat
     1501 cgagcggcta ctggccagtc ccaacgcaga cgcgaggatc gcggcgcggt tgttgtactc
     1561 ttgcaccgag gaggatgccg cgcgcgaagc cctcgacata atggccgaag cgtttcgtga
     1621 gcggaggatg ccggcggaac ttaacgacgg tttgcgtaag gaactgtcga tcgtactgca
     1681 cgagctgacc ggacaacgcg gcgacccgct tgccaccgat tatgcggttc tcgcggcgta
     1741 ttgcggcgac cgcgagagtc tcgatcaagc acgtcggatt gctcaatcga attcgttcga
     1801 cggagatgcg aggctgcgtg cgcttgcggc catcatgttt ctggaacggc cgtcttcgat
     1861 ccatccgatc gttgaaaaga tcttgaccga aagagatagc ccggactcga ctggatttcg
     1921 gggcaaggtg ctcgattcgc taggacgtct ggatgatccg gcgctcgcgc cgatcgtact
     1981 cgcggcgctc gccagcctgc cgcccgagct gaaaccccgc gtcatcgagc ttttgaccga
     2041 gcgttcctca tggagcaagg ccttgctggg ggccatcgag gccaagcaaa tcccgagctc
     2101 ggcgttgaac atcaatcagc tccgccggct gcagaagaac aaggatccag agatcaccga
     2161 ccgtgtgacg aagatttggg gcacgatccg ccaaggccgc aacccggacc gcgagcggct
     2221 ggtcgcgggc atgaagaatc tcatccgtac taccgccggc gatcccgtcg ccggccaagc
     2281 cgtcttcaac aagctctgct ctcagtgcca caaaatttac ggcgaagggc aagatgtcgg
     2341 ccctgacatc accgccaacg gccggaatga cttcgatcag ctcctgtcga acgtcttcga
     2401 tccgagcctc gtgatcggac cgggttacca agcaacgacg gtcgcaacca ccgaaggccg
     2461 tg
//
LOCUS       DATAQL010001074         6580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1483508, whole genome shotgun sequence.
ACCESSION   DATAQL010001074 DATAQL010000000
VERSION     DATAQL010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483508"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VHS97_18490"
     CDS             <1..729
                     /locus_tag="VHS97_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX3450249.1"
                     /translation="AFKKDVFTFVRIEYDSFGGRGGRRGGYGGGGWARWLTDFPDSDL
                     NFSLRLSQLTSLKVNPDPINMRLTDDRLFDYPFIYIIEPGALSFSEEEGVALRRYLFN
                     GGFLMVDDFWGDNELNNYLDQIKRVFPEDRYEPKELPLDHPIFRCVYPLKEKPQVPSI
                     GAWGGPGGPTYERGWGHGGDTQTVHYLGMEDDSGRLMSIICHNTDLGDGWEREGENAE
                     YFEEFSKKKSYPMGINIVTYAMTH"
     gene            790..1800
                     /locus_tag="VHS97_18495"
     CDS             790..1800
                     /locus_tag="VHS97_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3450250.1"
                     /translation="MAEDNDAYENERLAVEKVHESRGRIERELSKVIVGQKDATHQLL
                     ISLFAGGHCLITGAPGLAKTLLVRTIAQVFHLKFQRIQFTPDLMPADITGTEILEETT
                     DGRRRMQFVKGPIFANVILADEINRTPPKTQAALLEAMQEHQVTAAGVRYPLEEPFFV
                     LATQNPIEMEGTYPLPEAQLDRFMFNVIVDYLPEDEEVAVVQQTTSRQTEPIQPLFDG
                     RDVLRFHEVVRKVPIAEDLVRYAVRLSSSSRPGPEAPDFVTQWVSWGAGLRAAQSLVL
                     GAKARALLRGRFHVEPEDIRALVHPTLRHRILLGYRAEADGVGVDSVIDRLCEHVKGP
                     KH"
     gene            1800..2798
                     /locus_tag="VHS97_18500"
     CDS             1800..2798
                     /locus_tag="VHS97_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3450251.1"
                     /translation="MPSLERQGASGSASRRRDDQARGGSELIDPQALMRIKSLQVRAR
                     VAVEGFIRGIHRSPYHGFSVEFSEYREYTPGDDPRYLDWRLFARSDRYYVKRFEDETN
                     LRCYLVVDTSRSMGYRSVSFDKSVYARTAAATIAHFLAMQRDAVGLMLFEDRITEYLP
                     ARYRPGHLRRLMAALDRDPAGRATDLVGPLEEIAATVRKRGLIILISDLLAPTEALQT
                     RLGYLRSRGHDVVVIRVLDPAELDFPFTTPAMFRDVESGKDIYVDPSAARHDYLRRFK
                     AHAVAIERACVELGIEYVSITTDRPLELVLFDLLRARMRRGRQPGRRIAPGRGGAR"
     gene            2795..4900
                     /locus_tag="VHS97_18505"
     CDS             2795..4900
                     /locus_tag="VHS97_18505"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX3450252.1"
                     /translation="MSLLTPLYMLGLAALAAPIVFHLIRRAPKGDVPFSSLMFLSATP
                     PRLTRRSRLDNILLLLLRAGALALLVFAFARPFLRQAEHLDFGDVQERRIALLLDTSA
                     SMRRGDLWRQAQALARQVIDDCRPTDQLALYCFDGNWRPLLTFGESATLDLARRQTVA
                     RAHLERLTPTWGGTNLGGALVNTVAAIEDVADTSEKIAHMPRRVVLVSDLSQGSRLDA
                     LGDLEWPKDVELDIKTVSDSGSNAGLQLVADSAVADAAEADLSRRVRVVNNSGSAREK
                     FELAWLDDKGVTSGRPIDVYVPPGESRVVRVPRPAGNSPRQLLRLQGDSHGFDNNLYF
                     ADQLREEVTVEYIGADRGDDPDGLLYYLQRVFADSPRRQIRILSHSPSQAFDSDAKNP
                     PRLVILAAETPNNVSRLRAYLVGGGTILCVATAPGRAETLAGLAGVSPWEIAESATGG
                     DVMLGEIAFDHPLFAPFAGAQFNDFTKIRFKKYRRIDPDRLAGSRVIARFENNDAAVI
                     EKTIDRGRLCIMASGWQPADSQLARSSKFVPLMTALLEDRNPRPEAASKLVGEPVPLP
                     APIDESKKSVIVHKPDGTTVTLAPQNAIFDATDQPGLYAVDTPAGATWFAVNLDPLES
                     KTAPLPAETIEQYGIRVANHSQKTIDREQRRQMYNAELENRQKLWRWLILTAIGILIL
                     ETWLAGRAIDRPRLIRAEA"
     gene            4906..>6580
                     /locus_tag="VHS97_18510"
     CDS             4906..>6580
                     /locus_tag="VHS97_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450253.1"
                     /translation="MTMELRRALESVARRFRHVRLWAALALCWLAWAIAGTAMWLIES
                     GSGEKLVSDGWLLVLLGIVTATGVTCAIAALRSVKDFRWVARHIERKHPELNTGLLAA
                     IEEAAVTPSGPLGFLQFAVIRQALDHRRTHDWEETVPSWKLRGTQMVHAAALGFLVAV
                     GLALVGQARSLASPRTTIVSGASGGDVQVEPGNTEIERGSSLLVVARFKEGVPADASL
                     VVDDKSHPAASRGMTRSLEDPTFAGHVESVDTDLVYRVEFEGKSTPSFQVHVFEYPEL
                     QSTDAKLVFPRYTSMEPKTALDIRHVTAVEGTELTLLCHLNKVVATARLVDAKGQAIE
                     LKQVEADKPVYQATLTLADPGRFRVELVDREGRSNKLATEIVANVTRNRPPVVKLSQP
                     AHDVRVSPLEELKLKAEMEDDFGLVRHGLSYSMAGQEPREIVLRGPAANTRKLRAEHM
                     LDFESFHAAPDQLVTYFFWAEDIGPDGQPRRESSDMFFAEVRPFEEIFRQGEQPPSGS
                     AANEDQEGQDNARASDQLAELQKDIINGTWKLIRRETGAAPSPKLRDDGK"
BASE COUNT         1260 a         2007 c         2029 g         1284 t
ORIGIN      
        1 gcgtttaaga aagacgtctt tacctttgtg cgcatcgaat atgattcctt tggaggccgg
       61 ggcgggcgcc gcgggggata tggcggtggg ggttgggccc gatggctcac cgattttccc
      121 gatagcgacc tgaacttttc gctccggctt tcgcaactga cctcactgaa agtaaacccc
      181 gacccgatta acatgcggct gactgacgat cggcttttcg actatccgtt catctacatc
      241 atcgaaccag gtgccctttc cttttccgaa gaggagggag tggcgctccg ccgctatttg
      301 ttcaatggcg gtttcttgat ggttgacgat ttctggggcg acaacgagct gaacaactat
      361 ttggaccaaa ttaaacgggt ctttcccgaa gaccggtatg aacccaaaga gctaccactc
      421 gatcatccga tctttcggtg cgtttatccc ctcaaagaga agccgcaggt gcccagcatc
      481 ggcgcctggg gtggcccggg gggcccgacg tatgagcggg ggtggggcca tggcggcgac
      541 acgcagaccg tgcactatct agggatggag gacgacagcg ggcggctcat gtcgatcatc
      601 tgtcacaaca ccgacctggg cgacggctgg gaacgagagg gtgaaaacgc agagtatttc
      661 gaagagtttt cgaaaaagaa gtcttatccg atgggaatca atatcgtcac gtacgccatg
      721 acccattgaa aagactggac gctgccgccc cgagaagacg cctttagatc caagtcgaaa
      781 ggaacgagcg tggcggaaga caacgacgcg tatgaaaacg agcggctggc cgtcgaaaag
      841 gtccacgaga gccgcggccg gatcgagcgc gagctttcca aggtcatcgt cggccagaag
      901 gacgcgaccc atcagctctt gatcagtctg ttcgccggcg gccattgcct gatcaccggc
      961 gcgcccgggc tggcgaaaac gctgctcgtc cgtacgatcg ctcaggtttt ccatctcaag
     1021 ttccagcgga tccagttcac gcccgacttg atgccggcgg acatcacggg aaccgaaatc
     1081 ctcgaagaaa ccaccgacgg ccgccggcgg atgcaatttg tcaaagggcc aatcttcgcc
     1141 aacgtcattc tggccgacga aatcaaccga actccgccca agacgcaggc cgcattgctc
     1201 gaggccatgc aagagcacca ggtgaccgcc gccggggtac gctatcccct ggaagagccg
     1261 tttttcgttc tagcgaccca aaacccaatc gagatggagg ggacataccc actacccgaa
     1321 gcccagctcg accgcttcat gttcaacgtc atcgtcgatt acctgccgga agacgaagaa
     1381 gtagcggtcg tccagcaaac cacgtcgcgt cagaccgagc caattcaacc gttattcgac
     1441 ggccgggacg tactgcggtt tcatgaagtc gtgcgcaaag tgccgatcgc cgaggatctg
     1501 gtccgctatg ccgtgcggct ttcgtcgtcg agccgcccgg gccccgaggc gcctgacttt
     1561 gtgactcagt gggtgagctg gggcgcgggc ctccgggccg ctcagtcgtt ggtcttgggt
     1621 gccaaggctc gggcactctt gaggggacgg ttccacgtcg agcccgaaga catccgcgcg
     1681 ctcgttcatc ccacgttgcg ccaccgcatt ctgcttggct atcgggccga ggcagatggc
     1741 gtcggcgtcg attccgtgat cgatcgactg tgcgagcacg tcaaggggcc caagcactga
     1801 tgccgtcgct cgaacgtcag ggagcctctg gttcggcctc acggcggcgt gacgatcagg
     1861 cgcgtggcgg ttccgagtta atcgatccgc aggcgctcat gcggatcaaa agcctgcaag
     1921 ttcgggcgcg ggtcgccgtt gaaggcttca tcagaggaat tcaccgcagc ccgtatcacg
     1981 ggttctcggt cgagtttagc gagtatcgcg agtacactcc cggtgatgat ccccgttacc
     2041 tcgattggcg gctgtttgca cgctccgatc gctattatgt gaaacggttc gaagacgaaa
     2101 ctaacctccg ttgctacctc gtcgtggaca cgagccgatc gatgggctat cgatcggtgt
     2161 catttgacaa gagtgtttac gcgcggacgg cggccgcgac gattgcccac ttcctggcaa
     2221 tgcagcgcga cgcggtcggc ctgatgcttt ttgaggatcg gatcaccgag tatctccccg
     2281 cgcgctaccg gcccggccat ctcaggcggc tgatggcggc tcttgaccgc gacccggctg
     2341 gccgcgcgac cgatcttgtc gggccactcg aagagatcgc cgcaaccgtc cgcaagcgcg
     2401 ggttgatcat actgatctcc gacttgctgg ctcccaccga ggcgctgcaa actcggctcg
     2461 ggtatctccg gtcccgcggg cacgatgtcg tcgtcattcg cgtgctggat ccggcggaat
     2521 tggacttccc gttcaccaca ccggcgatgt ttcgcgatgt cgaatcgggt aaagatatct
     2581 atgtcgatcc cagcgcggca cggcacgatt atctgcgccg attcaaggcg cacgcagtcg
     2641 cgatcgagcg ggcgtgcgtc gagctgggca tcgagtatgt gtcgatcacc accgatcgcc
     2701 cgctggagct cgtgctgttc gacctcttaa gagcgaggat gcgccgcggt cggcagccgg
     2761 gccgcaggat tgcaccaggt cgtggaggtg cacgttgagc ctcctcaccc ccctttacat
     2821 gcttggtctg gccgcactgg cggcgccgat cgtgttccac ttgatccgcc gcgcgcccaa
     2881 gggggacgtt ccgttcagtt cgctgatgtt tctctcggca actcctcccc ggctgacgcg
     2941 gcgcagccgt ttggacaata tcctcttact cctgttgcgg gcaggagcac tggccctcct
     3001 ggtgtttgca tttgccaggc cgttcctccg ccaggctgaa cacctcgatt tcggtgacgt
     3061 tcaagagcgg cgaatcgctc tgctcttgga cacgagcgcg agcatgcgtc gaggcgacct
     3121 gtggcggcag gctcaggccc tggcccggca agtgatcgac gactgccgtc ccaccgatca
     3181 actggccctt tattgcttcg atggcaattg gcggccgctc ttgaccttcg gcgagtcggc
     3241 cacccttgat ctcgcacggc ggcagaccgt cgcacgggcg cacctcgagc ggctgacgcc
     3301 gacttggggc ggaacgaacc tgggtggagc tttggtgaac acagtcgccg cgattgaaga
     3361 cgtcgcggat accagcgaaa aaattgccca catgccgcgc cgcgtggtcc tggttagcga
     3421 tctgtcgcaa ggaagccggc tcgatgcact gggggatctc gagtggccaa aggacgtgga
     3481 gctcgacatc aagactgttt ccgactcagg ctccaacgcg gggctccagc tggtggcgga
     3541 ttccgccgta gccgatgccg ccgaggccga cctgtcgcgg cgggtccggg tcgtgaacaa
     3601 ttccggctcc gcgcgagaga aattcgaact cgcgtggctc gacgataaag gtgtcacgtc
     3661 gggaaggccg atcgacgtat acgtacctcc gggagaaagc cgcgtcgtgc gcgtgcctcg
     3721 tccggctggg aactcgccgc gccagttgct ccgcttgcag ggagactcgc acggcttcga
     3781 taacaatctg tacttcgccg atcagcttcg cgaagaagtc accgtcgagt acatcggcgc
     3841 ggaccgcggc gacgacccgg acggtcttct ctattatctc cagcgagtct ttgccgatag
     3901 tccccggcgc cagattcgaa ttctctccca ttcaccctct caagcgttcg attctgacgc
     3961 gaaaaatccg ccgcgactcg taattttggc ggctgagacg cccaacaatg tgagcaggct
     4021 ccgtgcatat ctggtcggcg gcggcacgat tctttgcgtg gcgaccgctc ctggccgggc
     4081 cgagacactt gccggtctcg ccggtgtgtc tccctgggag attgccgaat cggcgacagg
     4141 cggtgacgtg atgctggggg aaatcgcgtt cgatcaccca ctgttcgcgc ccttcgcggg
     4201 ggctcaattc aatgatttca ccaagattcg cttcaagaaa tatcgacgta tcgatcccga
     4261 tcgcctggcg ggttcgcggg tgattgcgcg tttcgagaat aacgatgcgg cggtcatcga
     4321 gaaaaccatc gaccgcggcc gactctgcat catggcgagc ggctggcaac ctgcagatag
     4381 ccagctcgct cggtcgtcga aattcgtacc gctcatgacg gcattactgg aggatcgcaa
     4441 tcccaggccg gaggcggcga gcaagctcgt cggcgagccg gtgccacttc ctgccccgat
     4501 cgacgagtcg aagaagagcg tgatcgtgca caagccggat ggaacgaccg taacgcttgc
     4561 tcctcagaat gcgattttcg acgcgaccga tcaacccgga ctctacgccg tagacacacc
     4621 ggccggcgcg acctggttcg cggtcaatct tgatcccctg gaaagcaaga cagcgcctct
     4681 gcccgccgaa acgattgagc aatacggaat ccgagttgcg aaccattccc agaagactat
     4741 cgatcgcgag cagcgtcggc aaatgtacaa cgccgaactc gaaaaccgcc agaagctctg
     4801 gcgttggctg atattgacgg cgatcgggat ccttatcctg gaaacctggc tggcgggtcg
     4861 agccatcgat cgaccccgct tgatccgcgc ggaggcatga ccgacatgac catggaattg
     4921 cgacgggcat tagaaagtgt cgcgcggcgt tttcgacacg ttcggctttg ggccgcgctg
     4981 gcgctctgct ggctggcctg ggcaatcgcc ggcacggcca tgtggttgat cgaatctggc
     5041 tcaggcgaaa agctggtctc ggacggttgg ctcctggttc tactgggaat cgtgacggcg
     5101 accggggtca cttgtgcgat cgccgcgctc cgttctgtca aggatttccg ctgggtggcg
     5161 cgtcatatcg agcggaagca tccggaactg aacaccggcc tcttggccgc gatcgaggaa
     5221 gccgccgtga cgccgtcggg cccactgggc tttttgcagt tcgccgtcat tcgccaggcg
     5281 ctcgatcacc gccggactca tgactgggag gaaaccgtgc cgtcctggaa gcttcgcggc
     5341 acgcagatgg tccatgcggc ggcgctcggc tttctggtcg cagtgggctt ggcgcttgtc
     5401 ggccaggctc ggtcgcttgc gagcccgcgc acaacgatcg tgtctggcgc cagcggtggc
     5461 gacgtccagg tcgagcccgg taataccgaa atcgaacggg gcagttcgct cctggtcgtc
     5521 gctcgcttca aggagggcgt gccggccgat gcgagcttgg tcgtcgacga caagtcccat
     5581 ccggccgctt cccgcggcat gacacgaagc ctggaagatc ccacgttcgc cggccatgtc
     5641 gaatccgtcg acaccgacct cgtgtaccgt gtcgaatttg agggtaagag cacaccgagt
     5701 tttcaggtcc atgtcttcga gtaccccgag ctccagagca ccgatgccaa gctggtattt
     5761 ccgcgttaca cgtccatgga gcccaagacc gccctggata ttcgccacgt cacggcggtg
     5821 gaaggaaccg agctgacgtt gctctgccat ctcaacaaag tcgttgcgac ggcgcggctc
     5881 gtggacgcca aaggccaggc gatcgagctc aaacaggttg aagctgataa gccggtgtac
     5941 caggcgaccc tgacgcttgc cgaccccggg cggtttcgcg tcgagctggt cgaccgcgaa
     6001 gggcgttcca acaagttggc gactgagatt gtcgcgaatg tgactcgaaa tcggccgccg
     6061 gtcgtcaagc tatctcagcc cgcccacgac gtccgggtat cgccgcttga ggagctcaag
     6121 ctcaaggccg agatggaaga cgatttcggc ctggttcgcc acgggctcag ctatagcatg
     6181 gccggccagg aaccccgcga gatcgtcttg cgaggccctg cggccaatac tcgtaagttg
     6241 cgtgccgagc atatgctcga ttttgagtct ttccatgctg cccccgatca acttgtgacc
     6301 tattttttct gggccgaaga cattggccct gacggccagc cgaggcgcga atctagcgat
     6361 atgttctttg ccgaggtacg gccgttcgag gaaatcttcc gccaaggtga acagcccccc
     6421 agcggctcgg cggcgaatga agatcaagag ggccaagaca atgcccgggc gagcgatcaa
     6481 ctggccgagc tgcagaaaga tatcatcaac ggcacctgga agctgatccg gcgcgaaacc
     6541 ggcgcggccc cgtcgcccaa gctgcgcgac gacggcaagg
//
LOCUS       DATAQL010001075         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_568528, whole genome shotgun sequence.
ACCESSION   DATAQL010001075 DATAQL010000000
VERSION     DATAQL010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568528"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1050)
                     /locus_tag="VHS97_18515"
     CDS             complement(43..1050)
                     /locus_tag="VHS97_18515"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3450254.1"
                     /translation="MAGFGPQIAPCPAAGRGDALEVLYRRVPEALRYRLIVEVLDEAN
                     RGEIDLSGLWAARERTGRILGALLTQPLAGKAAAVWAPEVLPSWRRPSLAAGLIQAAL
                     ADLKARGFLLAQAVLDESAGFRAARDLKRGGMPPITELLYLEKDTSLPLVIDRTEFVG
                     RDCAVGPRDNRTSSSFEWWPFEPALESEFRTVLQATYAGSLDMPELEGTRSLDDILES
                     HRAAGRFVAERWQLGRIPGKPEEASVLLMAEVPGRDIWEVVYLGLTPAARGRGLGRDV
                     LQHAIELARGHVSRLELAVDCRNIPAARLYQSAGFIARDRRTVHLAILGEPEEIFRRP
                     I"
     gene            1220..1960
                     /locus_tag="VHS97_18520"
     CDS             1220..1960
                     /locus_tag="VHS97_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450255.1"
                     /translation="MQKPAKPGLTHDAKRDFVKGARTPDAVEGVRPSPADESLGGPGL
                     VGSSDELNLNDAWLVEHARHGDHQAFAVLVRRYERKLIRVLTRLVREPEQAHDLAQET
                     FWRVYARLDRFDTARRFGPWLFRVGVNLGLDWLRSSKNELPPAASIDRTTGDGRATFE
                     LPDPDPRVHAELAQEVQFVLALMPVSYRTILVLRDLEGFSSSEVAAIVGRREATVRWR
                     LSKARDKFREIWERRQDGGRRETKDGRS"
     gene            1947..>2423
                     /locus_tag="VHS97_18525"
     CDS             1947..>2423
                     /locus_tag="VHS97_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450256.1"
                     /translation="MAEANMSKSMTSRECSWVRARLPLWVDQGVDEEQSEAIGDGRDL
                     SAKDSRVITQHLAECAACREHRLDLEQALGALAVAAAQLPVIPEGPSLWPELERRIAN
                     PKASDGARVPQGIDKAVRPLERWSELDGDRPIRRAWTRDTIRELFTSNRLHGPKSKR"
BASE COUNT          486 a          762 c          753 g          422 t
ORIGIN      
        1 tgccgctggc agccagagtt aacagccact aaccccgctg aatcaaatcg gcctacgaaa
       61 tatctcttcc ggttcaccga gtatcgccaa gtggacggtg cgacggtcgc gggcgatgaa
      121 tccggcagac tgatagagcc gggccgcggg tatgttgcga caatcgactg ccagttccag
      181 ccgagagacg tggccccgcg ccagctcgat cgcatgctgc agcacatcac ggccgaggcc
      241 ccggccccgt gccgctgggg tcaatcccag gtagacgacc tcccaaatgt cacggccggg
      301 aacctcggcc atcaagagca cggatgcctc ttcgggtttg ccggggatgc gtcccagctg
      361 ccaacgctca gcaacaaatc gcccggcggc gcgatgactt tcgaggatgt catccagcga
      421 gcgagtgccc tctagctctg gcatgtccag gctcccggcg taggtcgcct gcaagaccgt
      481 gcggaattca gactccaggg cgggttcaaa aggccaccac tcgaaggagc tgctggtgcg
      541 attgtcccgc gggccaaccg cacaatctcg cccgacaaac tcagtgcgat cgatcactag
      601 tggcagagaa gtatcttttt cgaggtaaag cagttcggta atcggcggca tgccgcctcg
      661 tttgaggtcg cgcgccgccc tgaagccggc tgattcgtcg aggactgcct gagcgagcag
      721 gaatccgcgt gccttcagat ccgccagtgc tgcctggata agccccgcgg cgagcgaggg
      781 ccggcgccac gatggcagaa cctcgggtgc ccataccgct gctgccttgc cggccagggg
      841 ttgcgttaac aaggccccca aaatcctgcc agtccgttca cgcgctgccc atagccctga
      901 cagatcaatt tcgccgcgat tggcctcgtc cagaacctcg acgatcagcc gatagcgcaa
      961 ggcctcaggg acccggcgat aaaggacttc aagggcgtcg ccgcggcccg ctgctggaca
     1021 tggcgcgatc tggggaccaa aacccgccat atcccttgac cccgcttcag gcccggccgg
     1081 accaccgcac ccgcaggaaa ttgagcgctg gctcccgtta atgccgcatc aagcccgttt
     1141 gcggaaaaca agctgggaaa aacatccagc gcgccgcggt caatttatcg tattataaag
     1201 tgcgtgagga ggtcacgcga tgcagaagcc ggccaaaccc gggcttaccc acgacgccaa
     1261 gcgagacttc gtcaagggtg cacgtacccc agatgcggtt gagggtgttc gcccgtcccc
     1321 tgccgacgag agcctcgggg gaccgggcct ggttggctcc agcgacgagt tgaacctcaa
     1381 cgacgcctgg ctggtcgagc acgcgcgaca cggcgaccat caggcgttcg ccgtgctcgt
     1441 ccgccgctat gaacgcaagc tgattcgcgt gctgacgcgg ttggttcgcg agccggaaca
     1501 agcacacgac ctggcacaag agaccttctg gcgtgtgtac gcccgtctcg atcgtttcga
     1561 taccgcccgg cgattcggcc cttggttgtt ccgagtgggg gttaatctgg gactggactg
     1621 gctccgcagc agcaaaaacg agctgccacc cgccgcctcg atcgaccgaa ccacgggcga
     1681 cgggcgggcg actttcgagc tacccgaccc tgacccccgg gttcacgccg agctggccca
     1741 ggaagttcaa tttgttcttg cgctcatgcc cgtctcctac cggacaattc tggtgctgcg
     1801 agacctcgaa ggcttttcgt cttcggaagt tgcagccatc gtgggacggc gtgaagcgac
     1861 ggtgagatgg cgattgtcca aggcacgtga caaatttcgc gaaatctggg aacgccgcca
     1921 ggatggtggg cgcagggaga ccaaggatgg cagaagctaa tatgagcaag tcgatgacaa
     1981 gtcgcgaatg cagttgggtg cgtgcacgcc tgcccctatg ggtggatcag ggggtggacg
     2041 aagaacaatc cgaagcgatc ggcgacggaa gagacttgtc tgccaaggat agccgcgtga
     2101 tcacgcagca cttggcggag tgtgccgcct gtcgcgaaca tcgtttggat ttggagcagg
     2161 ccttaggtgc gctcgcggtg gctgcggctc aattgccagt cattcccgaa ggtccatcac
     2221 tttggccgga gctcgagcgc cggatcgcca atcccaaggc atccgatgga gcgcgcgtgc
     2281 cgcagggtat cgacaaggcc gttcgaccgc ttgaacgctg gtccgagctc gatggggacc
     2341 gcccaattcg ccgggcctgg acccgtgata ccatccgtga gttgttcacg agcaaccgcc
     2401 tccacggacc caaatccaaa cgg
//
LOCUS       DATAQL010001076         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3774120, whole genome shotgun sequence.
ACCESSION   DATAQL010001076 DATAQL010000000
VERSION     DATAQL010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3774120"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..1547
                     /locus_tag="VHS97_18530"
     CDS             363..1547
                     /locus_tag="VHS97_18530"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3450257.1"
                     /translation="MSATHATQSSVPQEPVLYMAIELSLATWKLAFTVGSGQKPRIRS
                     VSAGALPCLNNEIELAKKRFGLPQDATVISCYEAGRDGFWVHRFLVSQEIKNIVVDSA
                     SIEVNRRKRRAKSDRLDATKLVSMLIRWHNGEKKVWAVVRVPTVADEDRRQLHRELID
                     LKGQRTEHANRIKGLLAGLGLRIVVDDQVPERLAALRQWDNQEVPPALRERVLREFER
                     WQLVGRQIRDLEAKRTEAIRNDRAPEVEKVRQLLRLRGIGENGAWLLVREFFGWRGIR
                     NRRELASLAGLSPTPYDSGGTRSEQGISKAGNRRVRWMMVELAWIWLRHQPTSDLSKW
                     FMRRFGQGNGRVRKVGIVALARKLLIALWKYLETGEVPAGAKLNGKLNGKKRILRLKK
                     VS"
BASE COUNT          457 a          561 c          653 g          376 t
ORIGIN      
        1 gctccgtcaa ccagagatag ttcgacggcc ccgttagcgc ggagaccggc ctccgcagcg
       61 aaggatttcg ggatgcggag ggccaggctg ttgccccatt tctgaacacg agtcttcatc
      121 gcgatctcca agtatctacg atgaagatgc acgcgcgacc agggggagtc aacgaggttt
      181 cggccaacga cctgcgtcag ccgccggatc aacggaaagc gacagaacat aacggacagc
      241 catcgcaaga tgccagattg ggcatccagg ggtcagatac catgccatag agcggtgtca
      301 acgtgagtcg ctgaggataa gctccagatg gtaattacaa cgtttcttcg aggagcttac
      361 ccatgtcagc gacccacgca actcagtcta gcgtcccgca agagcctgtg ctctacatgg
      421 cgatcgaatt gagcctggca acgtggaagc tcgccttcac cgtcggctcg gggcagaagc
      481 ctcggatccg ctcggtttcg gccggcgcgc ttccctgcct caacaatgag atcgagttgg
      541 ccaagaagcg attcggacta ccccaagacg ctaccgtgat ctcttgttac gaggctggcc
      601 gcgatggctt ttgggttcac cgattcttgg tgtcacagga gatcaagaac atcgtcgttg
      661 actctgcatc gatcgaggtg aatcgacgca aacgtcgcgc gaaatcggat cgtcttgacg
      721 ccaccaagtt ggtgtcgatg ctcatccgct ggcacaacgg cgagaaaaag gtctgggccg
      781 tagttcgcgt tcccaccgtt gccgacgagg atcgccggca gctccaccgt gagctgatcg
      841 acctgaaggg tcagcgcacc gaacacgcca atcggattaa gggactgttg gccggactgg
      901 gattgcggat cgtcgtagac gaccaggtcc cagaacggct ggcagctctc agacagtggg
      961 ataaccagga ggtgccgcct gcacttcgtg agcgagtcct gcgcgagttc gagcgctggc
     1021 aactggtcgg gcggcagatt cgtgatttgg aggccaagcg caccgaggca atccgaaatg
     1081 accgggctcc ggaagtcgag aaggtgcgtc aattgctgcg gctcagaggc atcggcgaga
     1141 acggggcgtg gttgctggta cgtgagttct tcggatggcg cgggatccga aaccgccgag
     1201 agcttgccag cctggcgggc ctgtcgccga cgccgtacga cagcggcgga acccgctccg
     1261 agcaagggat cagcaaggcc ggcaatcggc gcgtgcgctg gatgatggtc gagctggcct
     1321 ggatctggct gcgtcaccag cccacgagtg acctcagcaa atggttcatg cggcgattcg
     1381 gccagggaaa cggccgagtg aggaaggtcg ggatcgtcgc cctggctagg aaattgctga
     1441 ttgcactgtg gaaatacctg gagaccggcg aggtgccggc aggagcgaaa ctgaacggga
     1501 aactgaacgg gaagaagaga atcttgcgac tgaagaaggt gtcgtgacct acgctactcg
     1561 ggaattggaa agggctttga gaagaactcg tattagccgg tctcgggaat gaatcatggg
     1621 cagacagggc tggaggaggc gaccgaaact aggtcgggtt tcccgcgagg aagccgtggg
     1681 tcagatgggc tcgcctccgc cagttctggc ttcgagtcgt cggtagacgg atcagtaact
     1741 ggtgattgat cacttacgat caacacgaat agaagaccgt gccggcctca gggccggact
     1801 ctaagtgaag ccaccagcca ctgtcgttcc tgatcgaatt ccaggagacc agaaggagat
     1861 ttcgcccggt gcaatccgaa gctaacgaaa gacgtaacgt ggtgcgaaac ggtcggtctc
     1921 gggaacagtc cgcgtgcgcg ttgggggcgg gaccagaggc cccagttccc gcccccaaac
     1981 ccccaccccc ttccctctga aatgtggcaa gttagagctt tttattctaa ctctgaatgc
     2041 catgcag
//
LOCUS       DATAQL010001077         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4689077, whole genome shotgun sequence.
ACCESSION   DATAQL010001077 DATAQL010000000
VERSION     DATAQL010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4689077"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..2926
                     /locus_tag="VHS97_18535"
     CDS             383..2926
                     /locus_tag="VHS97_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX3450258.1"
                     /translation="MTGAFPRRLLVLVILSAFVAPARAADLVALSPQTWERYIPHGKE
                     VDGIYGDFALANDQLMAVVANPVLGRNANMTVRDVGGCLIDLTRRDRQSDQLSAFYPG
                     AQMRDLKFAGIEVEAPTMYEAAELDRVFVQARRISIRLVATPREQDPDVEVSYTLEDG
                     WPYVLVTTTFSNRGDRAVDADLVDAIRADRTFEFSPEAPAGLFWAYDKHFGQAYGVIA
                     DGHEIMSANARWLLLRYRDRDGKVAVRLGPGETYHLSRRIVPGSNLFDVRRVADSLAG
                     KEGRPVGLQVKDAAGRPVAGADVVMTMNGNRYSWGRANEEGRVLFNVGDAPSTLTVSA
                     LGHGSKEIALARDVPETLSVELDQAASVVARITDLEGRPIPCKVQFIGRDGTKSPDFG
                     PDSGEHAIKNVYYSHDGRFRRDLEPGPYDVIISYGPEYDAVFTRVDVERGKESVVEAK
                     LVRSVKTDHWISADFHSHSSPSGDNTSSQLGRVLNLLCEQVEFAPCTEHNRLSSYDPH
                     LRRLGAERRMATCVGMELTGRPLPLNHQNAFPLVMRPHTQDNGAPRADDDPAIQIERL
                     ALWDGASEKLVQVNHPDIGWMFRDRNGDGKADAGFVGMHGKMDVIEVHPPHTIFDKPT
                     TQAEGQTQNNTIVNWLQLLNTGRRIPGVVNTDAHYNFHGSGWLRNYLKSPTDDPALVR
                     TLDVVHAAERGNLIMTSGPFMEVNLRSSDASDTEAFPGDNLSARGGAATLKVRVQCPN
                     WFDVDRVQVFLNGRPSDELNFTRQSSPGRFSDATLKFDQDIPLHLERDAHVIVAAIGE
                     RSKLGPVMGPEHADDRPVAVSNPIFVDVDGGGFKANDDSLGELPVKRGQ"
     gene            3065..>3342
                     /locus_tag="VHS97_18540"
     CDS             3065..>3342
                     /locus_tag="VHS97_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450259.1"
                     /translation="MTRRSFPPAELMLCLVSLERALIDSRQLVAAFQTWRRAEGRTMG
                     EILVKKGFIAESRLAEVQEIVASRLRHRDGSLAEEPLSPGSSNSQSDE"
BASE COUNT          666 a         1021 c         1027 g          628 t
ORIGIN      
        1 cctcaatcca ccgtttcatt gtggcgcgcc taacggaaat agcaagtgaa ttgtttggag
       61 attatcaata tggacatgaa attaatcgca tatatggaat caacgcgaag cgcaccgcca
      121 acacgacgga ggcggtacgc ctgtgaacat ctcgtctggg ggcttcggca aacaggcaat
      181 gattcacgca gatttcactg taatcttgct gatatcggcg gttataagaa gacggtcgga
      241 agaaggtctg ctgcgccgct cgtggcgggc agaccaacga gcgccaagca aacgccggcc
      301 gacgagttgg tgatgtcaat ttccgtcaaa ggtcgctgga tcggtgaccg gtgcgacgat
      361 atcgatccgt gaggaactga gaatgactgg cgctttcccc cgaagattac tcgttctcgt
      421 aatactatcc gctttcgtcg ctcctgcgcg cgccgcggac ttggtcgccc tctcgccgca
      481 gacctgggaa cggtacattc ctcatggcaa ggaagtcgac gggatctatg gtgatttcgc
      541 gctggccaac gaccaactca tggcggtcgt cgctaacccc gtactgggcc gcaacgccaa
      601 tatgactgtc cgcgacgtcg gcggctgctt gatcgacctg actcgccgtg accgccaaag
      661 cgaccagctc tcggcgtttt atcccggcgc gcagatgcgc gatctgaagt tcgccgggat
      721 cgaggtcgaa gcgccgacaa tgtatgaagc ggccgaactc gatcgagtct tcgtccaggc
      781 gcggcggata agcatacgtt tggtcgcgac cccgcgggaa caggatcccg atgtcgaggt
      841 gtcttacacc ttggaggacg gttggcccta tgtgctggtt acaaccactt tttcgaatcg
      901 gggcgacagg gcggtcgacg ccgacttggt cgacgcgatc cgtgccgatc gcactttcga
      961 gttcagtccc gaggcgcccg ccggcctatt ctgggcctac gataagcatt tcggccaggc
     1021 gtacggcgtg atcgccgacg ggcacgagat catgagtgcc aacgcacggt ggctcttgct
     1081 gcggtaccgc gatcgcgacg gcaaggtggc cgtcaggctt ggccccggcg agacttacca
     1141 cctgtcccgt cgcatcgttc ctggctcaaa tctcttcgat gttcgacggg tcgccgacag
     1201 tctcgcgggt aaggaaggcc ggccggtcgg actgcaagtg aaggatgcgg cgggtcgtcc
     1261 cgtcgccggc gctgacgtag tcatgaccat gaatggcaat cgctattctt ggggccgcgc
     1321 caacgaggaa gggcgagtgc tcttcaacgt tggcgatgcg cctagcactt tgaccgtctc
     1381 ggctctcggg cacggatcga aggagatcgc gcttgcgcgc gacgtccctg agacgctctc
     1441 ggtcgagctc gatcaggccg cctcggttgt ggctcgcatc accgacctgg agggacggcc
     1501 gatcccttgc aaggtgcaat tcatcggccg cgatggcacc aagagtccag acttcgggcc
     1561 cgatagtggc gagcatgcga tcaagaatgt ctactacagc cacgatggcc gctttcgccg
     1621 cgacctcgaa cccggaccat acgatgtgat catcagttat ggaccggagt atgacgccgt
     1681 cttcacgcgc gtcgatgtag agcgcggcaa agagtcggtt gtggaggcga aactggttcg
     1741 ttcggtgaag accgaccatt ggatcagcgc cgatttccat agccattcca gcccctcggg
     1801 cgacaacacg tccagccagc tgggccgggt gctcaacctg ctctgcgagc aggttgaatt
     1861 cgctccgtgc accgaacaca atcgcctctc gagctacgat cctcacctcc gccgcctggg
     1921 ggccgaaaga cggatggcca cgtgcgtcgg gatggagctg accggccgcc cgttgccatt
     1981 gaaccaccag aacgcattcc cgctcgtgat gcggccgcac acccaggaca atggggcgcc
     2041 ccgggccgat gacgaccccg cgatccagat cgagcggttg gccctttggg acggcgccag
     2101 cgaaaagctg gtccaggtta accatcccga cattggctgg atgttccgcg atcgtaacgg
     2161 cgacgggaag gccgacgccg gtttcgtggg aatgcacggg aagatggacg tgatcgaggt
     2221 ccatccccct catacgatct tcgacaagcc gacgacacaa gccgaaggtc aaacgcagaa
     2281 caacacgatc gtcaactggc tccagctctt gaacacaggg aggcgcatcc ctggcgtcgt
     2341 caacaccgac gcgcactaca acttccacgg gtctgggtgg ttgaggaact acctgaaaag
     2401 cccgaccgac gatccagccc ttgtcaggac gctcgatgtc gtccacgccg ccgaacgcgg
     2461 aaacctgatc atgactagcg gacctttcat ggaggtgaat ctccgctcgt cggatgcatc
     2521 ggacaccgaa gcgttccccg gtgataacct ttccgcccgc ggcggggcag cgacgctgaa
     2581 ggtccgagtc cagtgtccga actggttcga tgtggatcgc gtccaggtct tcttgaacgg
     2641 ccggccatcc gacgagctca atttcacgcg tcagtcgtcc cccggccggt tttcggacgc
     2701 gacgctaaag ttcgaccagg acattccact ccatctggag cgcgatgccc acgtcattgt
     2761 ggcggcgatc ggcgagcggt cgaagctcgg accggtgatg ggaccggagc atgcggacga
     2821 tcggcccgtc gccgtctcga acccgatctt cgtggatgtc gatggcgggg gcttcaaggc
     2881 taatgacgac tcgttgggtg agctgcctgt gaagcgcgga caatgataga gcgccacgga
     2941 ccgcacgcac gccgaccgtc ctacaatcgc cggtcttgcc gaagcctttc tctcagtcga
     3001 caaaacggct aacctcaagg gtgctgagga acacccttgg agcccgcgac ggggaaacgc
     3061 accgatgacc cgacgctcct tcccgcccgc tgaacttatg ttgtgcttgg tctcgctcga
     3121 gcgcgcgctg atcgattcgc gccagctcgt tgccgccttt cagacctgga ggcgagcgga
     3181 agggcggacc atgggggaaa tcctcgtcaa gaaagggttc atcgccgaat caaggcttgc
     3241 cgaggtgcag gagatcgtcg ccagccggct ccggcatcgt gacggctctc tggccgagga
     3301 gccgctctcc ccagggtcga gcaactccca aagcgacgag ga
//
LOCUS       DATAQL010001078         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3467266, whole genome shotgun sequence.
ACCESSION   DATAQL010001078 DATAQL010000000
VERSION     DATAQL010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3467266"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VHS97_18545"
     CDS             complement(<1..451)
                     /locus_tag="VHS97_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX3450260.1"
                     /translation="MSQTTWPAFERTPAVAFVDVDGTLLAQTTTFLFARILYRKGWIR
                     RSFFLRALYHGLQHRFGRLDYGRLIAIGMNSIARIPVVELERIAYENFVEFVRPRLYE
                     GVVEHLYGLRRQGTAIVLVSSSPGLVIQPLSIYLSCTDTLTTPVVVER"
     gene            complement(448..>1758)
                     /locus_tag="VHS97_18550"
     CDS             complement(448..>1758)
                     /locus_tag="VHS97_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX3450261.1"
                     /translation="AAAYASECEEGAIVVGHDGRVSAPVFAAAVEAGITATGHDVITV
                     GATATPTIGVLVRDQNAAGGIQISASHNPPNYNGLKFFQRAGMVLSQRQGAQVLDRWQ
                     RRDFRWSSWDSLGKAQRLEDPDRPHLERVLSIVDSATIRRHSFKVVLDACHGAGGRLG
                     TATLRALGCKSTILGGQPDGLYDHTPEPTETNLKEFAAMVPAIGAAVGFAQDPDADRL
                     AIVDEMGRYIGEELTLALAATRRLEQVKGPVVLNLSTSRVTETLASRHGCPVLRTPVG
                     EINVVERMLAEDALIGGEGNGGVIDPRVGYVRDSFAAMALVLDLMAATGEPLSHLVAG
                     LPHYAMIKDQYPLGQNKALGSSSPTGASDIAALWDRIEAACPDARADRRDGLRLDWDD
                     RWVHVRASNTEPIVRVIAEAALAPLARELADRIGRWVRDTEGLP"
BASE COUNT          319 a          580 c          547 g          312 t
ORIGIN      
        1 cacgctcgac caccacgggc gtcgtcagcg tatccgtaca gctcaaataa atgctcaggg
       61 gctgaatcac cagtcccggc gacgaagata cgagcacgat cgccgtcccc tgacggcgca
      121 agccgtacag gtgttcgacg acgccttcat acaaacgggg tcgaacgaat tccacgaaat
      181 tctcgtaggc gatccgttcg agctcgacca ccgggatgcg tgcgatcgag ttcatgccga
      241 tggcgatcag ccggccatag tcgagccggc cgaatcgatg ctggagcccg tggtagagtg
      301 ccctcaaaaa gaatgagcgg cggatccagc ccttgcgata gaggatccgc gcgaagagga
      361 acgtcgtggt ctgggccagg agcgtgccgt cgacgtcaac gaaggcaacc gctggagtcc
      421 gttcaaaagc gggccaggtc gtctggctca tggcagtccc tccgtatcgc gaacccagcg
      481 gccgatcctg tctgccaact cacgggccaa cggcgccagg gccgcctcgg cgatcacccg
      541 gacgatcggc tcggtattgc tggcgcgaac gtgcacccag cggtcgtccc agtcgagccg
      601 caatccgtcg cgtcgatcgg cccgcgcgtc ggggcaagcc gcctcaatcc ggtcccatag
      661 cgccgcgatg tccgatgcgc cggttggcga actcgatcca agagccttgt tttggccgag
      721 gggatattgg tccttgatca tcgcataatg cggcaagccg gcgaccaggt gcgatagcgg
      781 ctcgcccgtc gcggccatca ggtcaaggac cagggccatc gccgcgaaac tgtcgcgcac
      841 atagccgacc ctagggtcga tcacgccccc gtttccctca ccccctatca gcgcgtcttc
      901 ggcgagcatc cgctcgacga cgttgatctc accgacaggt gtacgcaaga ctgggcaccc
      961 atgccggctg gccaaagtct cagtcacgcg ggaggtcgag aggttgagca cgaccggacc
     1021 tttcacctgt tctagccgcc gggttgccgc cagcgcgagt gtcagctcct caccgatgta
     1081 gcggcccatt tcgtcgacaa ttgccaagcg gtcggcatcg gggtcctggg cgaagcccac
     1141 agccgcgccg atggccggca ccatcgcggc aaactctttg agattggttt ccgtgggctc
     1201 gggcgtgtgg tcatagaggc catccggctg ccctcccaga atcgtgctct tacatccgag
     1261 ggcccgcagc gtggccgtcc cgagacgtcc gccagcaccg tggcacgcgt cgagcacgac
     1321 cttgaaactg tgtctcctga ttgtcgcact gtcaacaatt gagagcacgc gttccagatg
     1381 tggtcgatcc ggatcttcga gccgctgggc tttgccgagg gagtcccaac tcgaccagcg
     1441 aaagtcgcgt cgctgccagc ggtcgaggac ctgggcgcct tgtcgctggc tcaaaaccat
     1501 gcctgctcgc tggaagaact tcaggccgtt gtagttgggc ggattgtgcg aagcggatat
     1561 ctgaattccg ccggccgcgt tttggtcgcg caccaacacg ccgatggtcg gcgtggccgt
     1621 cgctcctacc gtgattacgt cgtgcccggt cgcggtgatg ccagcctcga ccgcagctgc
     1681 gaacaccggg gctgatacgc gtccgtcgtg acccacgacg atggccccct cctcgcactc
     1741 gctggcgtag gccgcggc
//
LOCUS       DATAQL010001079         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3162544, whole genome shotgun sequence.
ACCESSION   DATAQL010001079 DATAQL010000000
VERSION     DATAQL010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162544"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            619..1302
                     /locus_tag="VHS97_18555"
     CDS             619..1302
                     /locus_tag="VHS97_18555"
                     /inference="COORDINATES: protein motif:HMM:NF042726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6655 family protein"
                     /protein_id="HEX3450262.1"
                     /translation="MQRNCTIRRRARGLALLGVVLVSGCTSVTVTGTPRTGSEQLLLT
                     GTFDDAIYHVDFSPLTGSKVFLDTQYVTIVDKDWVISSIRRTMAEQGIMLENNKDKAE
                     VIVEAAFGAYGTDQRDRKFGLPGISLTPSLTTGAAVTAAGTSNALNLSETNQQDAVVK
                     ARLYAYDAKTGRLVWETAPLLNAQGLRDHFVIGSGPYRLSSRPEVQQYPAESQSQTRK
                     HLFHQLFGH"
     gene            complement(1347..2225)
                     /locus_tag="VHS97_18560"
     CDS             complement(1347..2225)
                     /locus_tag="VHS97_18560"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX3450263.1"
                     /translation="MPSKAESEYRYEKLTWPEIDDAVELGKVCILPCGAVEQHGPHLP
                     LDVDVICPTEIARGAGGIIPEKMLLLPTHCYGYTGHVMDFPGTINTHFQHFIDGILDI
                     TKSLAYHGFKKIILLNGHGSNWPNLDLVARRTNLETDAECVPLCWWGLLTVDKEFLPR
                     WRQSKFPGGCSHACELETSLYLYLAGDDVRKDQIKSGTVSFNDESSPFQWVDLFAAGP
                     ATVVSWTSSYSETGVLGDAELATAEKGRLAYDESVTQLVRFVSWFKDRPQDRRRDRHR
                     KPPTMPIPWGQKPFHT"
     gene            complement(2338..2718)
                     /locus_tag="VHS97_18565"
     CDS             complement(2338..2718)
                     /locus_tag="VHS97_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450264.1"
                     /translation="MDQATLVDHQIDDVPKLIDQLRLDNFDVEAAFWLYTSEADQWFL
                     YLVSDAVDRVGTTQAYKSVYRSMRRLTGLWINPFEVKLVGPADPVAKAVVDHRAFAHA
                     PLPTRVRGSYLGNVYIENAYIYYI"
     gene            complement(2802..3113)
                     /locus_tag="VHS97_18570"
     CDS             complement(2802..3113)
                     /locus_tag="VHS97_18570"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX3450265.1"
                     /translation="MNRIKYQELADLRIDEAVVLLSQGKYDGAYYLAEYAVECGLKAC
                     IAKLTNQYDFAPKPKFIHDYYSHEIERLVKTAGLTAQRDTDAATDPDLQANWGIVKDW
                     T"
     gene            3307..>3653
                     /locus_tag="VHS97_18575"
     CDS             3307..>3653
                     /locus_tag="VHS97_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3450266.1"
                     /translation="MKFCGRFGWMLFATTVMNSLTFGQEPRPEPFDILIRGGKIVDGT
                     GNPWFEGDVAIRGDRIEAVGSPDPRVAARHTIDATNLIVAPGFIDMHSHSDMTLLEDG
                     SAQSKIRQGVTTEV"
BASE COUNT          780 a         1025 c         1026 g          822 t
ORIGIN      
        1 ccgatatcct cggcccccat taccatgaga ccgacctgct aagtcagcca ctagacctcg
       61 acccgcacgg agccaaggta cccgacggcc ccggcctcgg cgtctccctc gatgaagacc
      121 agctcaagcg cttccgggtt gactgaaccg ccaagaagag aacacgatta gaagaattta
      181 aaccgccaag accgccaaga ccgccaagaa agacagaata gtgcagattt tacatattat
      241 taaaattcca attgcattct gtgtttttgc atctccttcc ttttttcttg gcggtctcgg
      301 cgttcttggc ggttgattag attttggatc tctttgttgt cactacattt gtggagcgct
      361 tgactggctc gccttttgtt ctcgcagatc gatacaacgt ggcagttaga aagtagattc
      421 aagttttcct ggcggtaatg ccgacttggc gttgcccgac tgggctcgca gggctatact
      481 tcgcgcggtg agatcgaccc accggtgaac agatcgatag aaaaccgcac ggaagccatg
      541 ggacgactca gaatcaactc atcgactcct ttctggatcc gccgttgccg gtgcaccggt
      601 tgatgatggc ggttgatcat gcaacggaac tgtacgatca ggcgacgtgc gcgtgggcta
      661 gccctcctcg gtgtggtgtt ggtgagcggc tgtacgagcg tcacggtcac tggaacaccg
      721 cgcaccggta gcgagcaact ccttctcacg gggactttcg atgatgcaat ctatcacgtc
      781 gacttcagtc cgctcaccgg gtcgaaggta tttctcgaca ctcagtatgt cacaatcgtc
      841 gacaaagact gggtgatctc cagtattcgt cgcacgatgg ccgagcaggg cattatgctc
      901 gagaacaaca aggacaaggc cgaggtgatc gtcgaagccg cgtttggcgc ctatggcacc
      961 gaccagcgcg atcgcaagtt tggtcttccg ggcatcagcc tgaccccgtc gttaacaacg
     1021 ggcgccgccg tgacggccgc aggtacatcc aacgcgctga acctgtcaga gaccaaccag
     1081 caagacgccg tcgtgaaggc gcggctgtac gcgtacgacg ccaaaaccgg gcgacttgtt
     1141 tgggagacag cgccactctt gaatgcgcaa ggtctccgcg accacttcgt gatcggttct
     1201 ggcccttatc gtttgagctc acgtccggaa gtccagcaat atccggctga atcgcagagc
     1261 cagacacgta agcacttgtt ccaccaatta tttgggcatt gagcccggat ggtcggcggg
     1321 tctttacccg gcgatgatgt cgggccttat gtatggaagg gcttctgccc ccaagggatc
     1381 ggcatcgtcg gcggtttgcg gtgccggtcg cggcgccggt cttggggacg atctttgaac
     1441 cagctcacga agcggaccag ttgggtgacc gattcgtcgt aggccaggcg gcctttctcg
     1501 gccgtcgcca gctcagcatc gcccaaaacg cctgtctctg aataactgct ggtccacgag
     1561 acgaccgtcg cgggaccggc ggcgaagagg tcgacccatt gaaatgggct cgattcgtcg
     1621 ttgaacgaga ccgtgccgct cttgatttga tccttgcgga cgtcgtcgcc agctaagtaa
     1681 aggtagagcg acgtttcgag ctcgcacgca tgggaacagc cgccgggaaa cttgctctgc
     1741 cgccagcgcg gcaggaattc tttgtcaacg gtcaagaggc cccaccagca taaaggcaca
     1801 cactcggcat cggtctcgag attggtccga cgtgcgacca ggtcgaggtt gggccagttc
     1861 gagccgtggc cgttcaagag gatgatcttc ttgaacccgt gataagccaa gcttttcgtg
     1921 atgtcgagaa ttccatctat gaagtgctga aaatgcgtat tgatggttcc cgggaagtcc
     1981 atcacatgcc ccgtgtaacc ataacagtga gtgggaagca aaagcatctt ttcgggtatg
     2041 ataccgccgg cgcctcgggc gatctcggtg gggcagatca catcgacgtc gagtggcaga
     2101 tgcgggccgt gctgctcgac ggcgccacaa ggcaggatgc agaccttgcc cagttcgacg
     2161 gcgtcgtcga tctcgggcca cgtgagtttc tcgtagcgat attcggattc tgccttgctg
     2221 ggcatctttg gagttccgta gttcttgtat ggcgggatga cgccgagcct tttacacaat
     2281 cgagccgcgc cccctggggc gggtcccgga tcgatgtccc actagatatt tgtgatccta
     2341 tatataataa atatacgcgt tttcgatgta gacatttcca agataggagc ctctaacacg
     2401 cgttggcaga ggagcatggg cgaaagcccg atggtccacc acggctttcg cgaccggatc
     2461 tgccggtcct accagcttca cctcgaaagg attgatccac agacccgtca atcgccgcat
     2521 cgaccggtat acgctcttgt aggcttgggt cgtccccact cgatcgaccg cgtctgacac
     2581 gaggtaaaga aaccactggt ctgcctcgct cgtgtaaagc cagaaggcgg cctccacgtc
     2641 gaaattgtcc agcctgagct gatcgattag cttgggcacg tcgtcgatct gatgatctac
     2701 cagcgttgct tgatccatgg gaacaccccg tgtgcggcgt ccgtgattgc cgtgataagt
     2761 tcctgggcct gaatctgagt cttgcgctca taccggcttt cttaagtcca gtctttgacg
     2821 attccccagt tggcttgcaa gtcgggatcc gtggcggcgt ctgtgtctcg ctgtgccgtc
     2881 agaccggcag tcttgaccag cctctcgatt tcatgcgaat agtaatcgtg tatgaacttc
     2941 ggtttgggag caaaatcgta ttgattcgtc agcttggcga tgcaagcttt gagaccgcac
     3001 tcgacagcat attcggccag atagtacgcg ccatcatact tcccctggga caatagtacg
     3061 accgcctcat cgattctcag gtcggcgagc tcctggtact tgatacgatt catcacggca
     3121 attccgttgg cgtcgatcag tcgtagtttt atggtaagcg aagcgattcg tattgtggaa
     3181 gccgagacgt tcgatcgcga gattctgtcc ctaagtctgg cttgcgtgct cactcgaaga
     3241 taaaacaaat gccaagatag tcccgattct tcaatcgccc ggggaaccac aaaaccagga
     3301 gcaccgatga aattttgcgg acgtttcggc tggatgctat tcgccacgac ggtaatgaac
     3361 tctctcacgt tcggtcagga accgcggccc gaaccgttcg atatcctcat ccgaggaggc
     3421 aagatcgttg acggtaccgg caacccctgg tttgaaggcg atgtggcgat tcgaggagat
     3481 cggatcgagg ccgtcggatc gcccgatccg cgagtcgctg cccgccacac gatcgacgca
     3541 acaaatctga tcgtcgcccc cggattcatc gacatgcact cacattcgga catgacgcta
     3601 cttgaagacg gctccgcgca aagcaaaatc cggcagggag ttacgactga agt
//
LOCUS       DATAQL010001080        14843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_262197, whole genome shotgun sequence.
ACCESSION   DATAQL010001080 DATAQL010000000
VERSION     DATAQL010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 14843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14843
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262197"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..121
                     /locus_tag="VHS97_18580"
     CDS             8..121
                     /locus_tag="VHS97_18580"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX3450267.1"
                     /translation="MPLELLRRIVGCSSNPGDLVCDPFTGSGTTPVVCQEL"
     gene            complement(187..1203)
                     /locus_tag="VHS97_18585"
     CDS             complement(187..1203)
                     /locus_tag="VHS97_18585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3450268.1"
                     /translation="MPRQAKLWKRAGRAGWWATISGRKVMLGLEFADAEREFHRRKAA
                     GKAVPCSRLTVEALVDLFLDAMRGEIKESTWTNYRWHLQRWCNHSGSKIASQLKPFDA
                     AAWLKSETSWNSSTKRAGTEIIRQWSRWCKNQGYLDADRLAGTRYPKSIPRAAAPMDD
                     IERFIAGIREERFRDLVLVLLDTGARPGEIRTLTAARIDWKASSAVVEGKSGARVIGL
                     TARSLEILRRAADQFPDGPVLRNGKGDPWQAGAMTQRFRRVRNRVNVHVVPYHFRHAL
                     FGRASKAGVDSVVIARQLGHANLNMLLKVYSHVDAEQMRAAVDKTASKTQDADETTPE
                     QGAA"
     gene            1405..2067
                     /locus_tag="VHS97_18590"
     CDS             1405..2067
                     /locus_tag="VHS97_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3450269.1"
                     /translation="MRITWYGHAAFLIETQGLRIILDPYRSPDCGGYEPIAEGADLVV
                     VSHENDRYHSHLGQIVPPFEVVRALELPSEGKVVRGVRFEAVPVFETPERLVEDKVTI
                     IHFRCEDLHLVFLGDLGHRLSDEELAPLRGAEIVLAAAGGPPTIDFPEIPPLLEAIDP
                     RIVVPMHYKTPKINLDIQPLPRFLEALPGWRVERPGNNSVEITRAKLPAQQTIVALEY
                     AR"
     gene            2287..2475
                     /locus_tag="VHS97_18595"
     CDS             2287..2475
                     /locus_tag="VHS97_18595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX3450270.1"
                     /translation="MDVQTRLELIGLIWDSIDENEESIATPEWHLRELELRREAADAN
                     PDAAVPWELVKSRFEREQ"
     gene            complement(2676..3839)
                     /locus_tag="VHS97_18600"
     CDS             complement(2676..3839)
                     /locus_tag="VHS97_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3450271.1"
                     /translation="MIPLKYNVRNLRVRWKTTLMTVMGTGLVVWSSCILFGLVEGLEH
                     SLSISGDPLDLIVMRKGSSTETTGGFETSKADEILILPGILRDREGQPIAAKELLNIP
                     IAERENGTKTNIIIRGVQPASRRLRPSFTIVAGRDFVAGKGECIVSKRMAARFKGAQV
                     GGRLVFGSKEVYDVVGIFTAGGSAAESEIWADLKDVEKNTGRDGSVSCVQLRAASSAD
                     CNKIKQTIDDENRFRLAAIPEPLYFETQSRSGIFLKGAGTLIAVLLTFGAMFSSANTM
                     FAAVSARTREIGTMRALGFSQLDILISFLGESLLLCALGGAIGLLATFPLNALTYETS
                     DFNSFASVTVAFRFGPLVMTVALVMTLAMGVFGGMFPALRAVRLDVISALREL"
     gene            complement(3847..5040)
                     /locus_tag="VHS97_18605"
     CDS             complement(3847..5040)
                     /locus_tag="VHS97_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3450272.1"
                     /translation="MKYLTYILRNARRNPVRSILTIASMSICLSLMMILLSFFAISDE
                     ANSSTRIFNRIASLNANGFAGVLPITRVKEIAQLDGVVAASPFSWIGAKYQDEIMPFA
                     QFGVDPDTVFKVMDELTIPPDQLKSFQASRDGCVIGRKLAEDKKLTVGSKLPLKGDAY
                     PVDLDLTVKGIYDGPSNRDLRMCMVRFEYLDELLKRRLSNSTSATTGSGQRSGNAGMI
                     FIKCKTADDMPLLCKTIDESYRNSDFPMRTQTEEAFGKMFEEMLGDLKGMIRIIGLAV
                     IFSLLCVASNSMAMSMRERTSEVAVLKAIGFTRRLILFMVLTEAVLIAGFGGAIGSLG
                     VKAFCDWVDLSRFTGGFLPFYYVPWNIALQGLAVSVFIGLASGLYPAVRAANLSVIDG
                     LRRVI"
     gene            complement(5037..5753)
                     /locus_tag="VHS97_18610"
     CDS             complement(5037..5753)
                     /locus_tag="VHS97_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3450273.1"
                     /translation="MAEMSSIVLRGVYKEYRRDEFRIPVLVNLDVSVGEGEFLALMGP
                     SGSGKTTLLNLIAGLDRATSGEVMVHGQDLGDLSEAEITRWRANNVGFIFQTYNLIPV
                     LTAFENIELPLLLTHLSRRKRRENVMTALRVVGLTGREHHYPRQLSGGQEQRVAIARA
                     IITDPYLLVADEPTGDLDRTTGGEILDLLEQLNRDFQKTIVMVTHDPLAAQRATRTLY
                     LDKGRLVNETVPGSPISAAS"
     gene            complement(5792..7075)
                     /locus_tag="VHS97_18615"
     CDS             complement(5792..7075)
                     /locus_tag="VHS97_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450274.1"
                     /translation="MAGTLRDELASLKIDRPNPIKSGRNGYRESSRRRGGSGLRLLSW
                     MLWLIPLGLLAAGGVYGYIQYDTMRSRPLVKKGLVVSKTAAEAATLLDANGYLKSRYQ
                     AMIGTKIAGRVEQMAVEEGMKVKKGDTLAVIEHNDLKAMLASREAQAQRTQAELEEAR
                     ADLWEKEREDRRASRLFAQRSITPEESEKAQAGHKKAAARVLALEAAVKLMKANVEEI
                     KATIVTMHLYAPFDGTVVEKQGEVGEIISPTAMSSSLGRTAVVTIANLEKMDVETKIE
                     EGLLSRITLGQPAVVSVSAIPSKRYRGRLRQVLPMSDRANAVVKVKVEILDPDDRLFP
                     ELAAKVSFLRSESVINPDADRPFLFVPKSAVFQEDGRDFVWVIRDDNTLRMYRVEVVS
                     TTDDWARVESGLKLNDAVVLGPMKTLREFDVVRID"
     gene            complement(7202..8215)
                     /locus_tag="VHS97_18620"
     CDS             complement(7202..8215)
                     /locus_tag="VHS97_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450275.1"
                     /translation="MRKLGATMSASPSRLRSASTKGSSQVPIARPPVPAPGIVPDGEP
                     DSPLEAEWWTPEALKGWAGSMTLHAVLLVVLACWYFAPRTKGSITFDSRLAGSLKGAP
                     EGEMLTGGLSTPLTLSAAPLVEKVLGLDSPALGQLELAPPEPEVRLRLAKNPSGGGGK
                     ANDNPGAGDGDGFGLARFGEGGELIRGVSVKVGDPQFTLIWNSDGVDLDLHVLEPGGK
                     EIYWEEPKGRQGGELDVDNTKGFGPENIYWMVESQGPGSEKVKGPGPPGTYKWFVVYW
                     GGFGGVPRPTHWQVRVKHAGKVDVFHGKFSRLNERSKIYTLTVDPPGGKTAEPAHSVG
                     GSP"
     gene            8477..9265
                     /gene="larB"
                     /locus_tag="VHS97_18625"
     CDS             8477..9265
                     /gene="larB"
                     /locus_tag="VHS97_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX3450276.1"
                     /translation="MDSHELKELLDAVRDGRVPPGVAAERIQVQPYQDAGGFAKVDLH
                     RRLRCGFPEVVFGQGKTAAQIEAILKTLLEHEQGGLVTRLDPQAAPHLLAAFPHGEHN
                     SLGRTFRVRSSEDEGPKLGRVVIVTAGTSDLPVAEEARVTAEAWNCDVTLVADVGVAG
                     LHRLLHQLPQLKTADALVVVAGMEGALPSVVGGLVECPVIAVPTSIGYGAHFQGVAAL
                     LGMLNSCASNVVVVNIDAGFSGGHVASLIARRMGQARQAAARPL"
     gene            9358..10257
                     /locus_tag="VHS97_18630"
     CDS             9358..10257
                     /locus_tag="VHS97_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX3450277.1"
                     /translation="MTLEKVDTVPNLPARPRDSHKGRFGSVLIVAGSRGMAGAAALCG
                     GAALRSGAGLVRVATPSEIQPTVSSFEPSYMTYPLPCDENGLIRFGPARPVLERLIGH
                     ADVVAVGPGLGQSDDIQQLVGFLIASAQKPLVIDADGLNALVGQTDLLSQRKCPVVLT
                     PHPGEFARLTGSTITEVQSDRVQRAAALATLSSELVVVLKGAGTVVTDGNKYYINTTG
                     NPGMATGGTGDVLTGVIAALLAQKLPPFDATQLGVFIHGLSGDIARDQSGEIGLIAGD
                     LVDALPDAFVHAMPDPDPISISN"
     gene            10470..10982
                     /locus_tag="VHS97_18635"
     CDS             10470..10982
                     /locus_tag="VHS97_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450278.1"
                     /translation="MSTRENPSSPAPAWTYRMEVSPTGQSPTAAIANPNDPILLLHLL
                     ANLQSQTLEVQRQMLEMQRQQLEISRELIQVNREQRARQGAELERWQAGHEPILETCR
                     ETLGKLEQVHASLMSELANYVDDNHENLLEGDFSLSDFVDRFGPRLAHLNTMLAVLRP
                     LAAAKIKTES"
     gene            complement(11257..11643)
                     /locus_tag="VHS97_18640"
     CDS             complement(11257..11643)
                     /locus_tag="VHS97_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX3450279.1"
                     /translation="MVGKKLYVGNLTYRVSSSDLEQLFSQFGSVESAEVISDRDTGRS
                     KGFGFVEMSSDNEARAAIEALNDQEHDGRRLTVNEARPREPRTGGGGGGGGGWRGGGG
                     GGAGGGGGAGGGGGRGRSGGYDTRRY"
     gene            complement(11897..12436)
                     /locus_tag="VHS97_18645"
     CDS             complement(11897..12436)
                     /locus_tag="VHS97_18645"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3450280.1"
                     /translation="MLTLGSNRLILRMFRESDLDAYAAMCGDPDVMRYLGDGYPLTRA
                     EAWRNMALILGHWQLRGFGFWAVEERATGILAGRVGCWRPEGWPGMEIGWALRREYWG
                     RGYATEAAQTVMADAFSRMGQNRLISLIRPENGPSIAVALRLGMRKEWSTEIMGHPAL
                     VYGIERTINPTPRTVVMPL"
     gene            complement(12504..13442)
                     /locus_tag="VHS97_18650"
     CDS             complement(12504..13442)
                     /locus_tag="VHS97_18650"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HEX3450281.1"
                     /translation="MNKTVKTLAALASATVVLSFVVVVINQTAGVVQLAKEVHPALGS
                     VTLWTLLIAYGGLIGVPVVMVMRMPRALVPPATESGPEFDEHLKQLGQRLAVNPRVQR
                     ASIAPFDRAGVEDALQVLDDDANAIVKQMATTVFLTTAVSQSGRLDAVLVLAAQSRMV
                     YRVARLYYQRPSLRELAHLYANVAATSFVAGELDDLELHQMIQPVVAGTVGALGGAIP
                     GFQIMTSIMVNSLLSGSANAFLTLRVGIIAKDYCGSLVAEPRTQVRRAATAEAARLLS
                     GIVRDSGTRVREAIWKDLKTKFPRPWPLRKSQPETA"
     gene            complement(13654..>14843)
                     /gene="argJ"
                     /locus_tag="VHS97_18655"
     CDS             complement(13654..>14843)
                     /gene="argJ"
                     /locus_tag="VHS97_18655"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX3450282.1"
                     /translation="QLAIPRGFRASGVKAGLKASGSLDLAVLAADQPCAAAGTFTTNR
                     VCAAPVKWCRELLPADSIRAIAINAGNANAATGEQGLANARRTAERVATLLGCQPEEV
                     LVASTGIIGHQLAMDKIEAGIEKAVAGLSDRPEAFQTVAESIITTDTRPKIVSLRETI
                     DGREVALLGIAKGAAMIGPRMATMLGFLLTDAPVWANELQDILADAVEHSFNCLSVEG
                     HASTNDTVLLLSSTATTEQVLRGESLAAFAAMVRSTCETLARAIADDGEGATHFITID
                     VEGCETFEGARAIARAVADSPLVKTAIHGADPNWGRIVSAAGYAGIAFQEEELSLWIN
                     SVAVYKDGSPIPFDDAGLSRSLRTERNVDLRLRFKSGKECVRFWTCDLTAEYIRLNAD
                     YTT"
BASE COUNT         2957 a         4597 c         4362 g         2927 t
ORIGIN      
        1 gactcaattg ccgcttgagc tgttgcgccg catcgtaggg tgcagctcga atcccggcga
       61 ccttgtttgc gatcccttca cggggtccgg aacgactccg gtcgtttgcc aggaattgtg
      121 acggcgattc attggggccg agctgtcgac tgcgtacgtc gagatttcgc gtgccaggct
      181 tgcagctcac gccgccccct gctctggcgt cgtctcgtcc gcgtcttgag tcttgctggc
      241 cgtcttgtcg accgccgccc gcatctgctc cgcgtcaacg tgcgaataca ccttgagcaa
      301 catgttcagg tttgcgtgtc cgagctgacg cgcgataacg acgctgtcga cgcccgcctt
      361 tgatgccctg ccgaatagtg cgtgcctaaa atggtatggt acaacgtgaa cgttgacgcg
      421 atttcgcacg cgcctgaatc gctgcgtcat cgctccagct tgccacggat cacccttgcc
      481 gtttcgcagt acggggccat ccgggaattg gtcggcggct cgacgtagaa tctcgagcga
      541 cctcgctgtg agcccgatca cccttgcccc gctcttccct tcgacgactg ccgacgacgc
      601 tttccagtcg atccgtgcgg cggttaacgt gcggatctcg cctggtctgg cgcccgtgtc
      661 gagcaggacg agtacgaggt cgcggaaacg ctcctcgcga atgccggcga tgaagcgctc
      721 gatgtcatcc atcggcgcgg ctgcccgagg gattgacttc gggtatcgcg tgccggcgag
      781 gcgatccgcg tcgagataac cctgattttt gcaccatcgc gaccactgcc ggattatctc
      841 cgttccggcc cgcttagtgg acgagttcca cgatgtttca gacttgagcc acgcggcggc
      901 gtcgaacggc ttgagctgcg acgcgatctt agaacccgag tgattacacc accgctggag
      961 atgccatcgg tagttcgtcc aggtcgactc cttgatctcg ccgcgcatgg cgtcgaggaa
     1021 taggtcgacg agggcctcga cggtgagcct tgagcacggg acggccttcc cggccgcctt
     1081 gcgtcgatgg aactctctct cggcgtcggc gaattcgagt ccgagcatca ccttgcgacc
     1141 gctgattgtc gcccaccagc cggcgcggcc ggcccgcttc cagagtttcg cttgcctggg
     1201 catccctgcc tcctttcggg ggctgataag tgcgctaaat ggtgccaagc gtcaatacga
     1261 cattggattc actctggaat ataagccata gaacgaaaat ggcaactctt tgtaacacaa
     1321 tgacgcacgc cgagtttatg atcgaactgg acatcggagg aattcgacca gactggattg
     1381 attggccggc accgaggaag gctcatgcgc atcacgtggt acgggcacgc ggcgtttttg
     1441 attgagactc agggactgcg gatcatactt gacccgtacc gttctccgga ttgcggaggc
     1501 tatgagccaa tcgccgaggg agccgacctg gtcgttgtca gtcatgaaaa cgaccgttac
     1561 cacagccact taggacagat cgtcccgccg tttgaagttg tccgcgcgct cgagctacca
     1621 tccgagggca aggtggtccg cggggttcgt ttcgaggcag ttccggtttt cgagacaccg
     1681 gagcgcctgg tggaagacaa agtgacgatc atccatttcc gctgtgaaga ccttcacctg
     1741 gttttcctgg gcgacctggg ccatcgcctt tccgacgaag agttggcacc gttaaggggc
     1801 gccgagatcg tcttggcagc cgccggcggc ccgccgacga tcgactttcc cgagatccct
     1861 cctctactcg aggcgatcga cccgcgcatc gtcgtgccga tgcattacaa gacgcccaaa
     1921 atcaacctcg acatccagcc gctcccgcga tttctggagg cgttgcccgg ctggcgcgtc
     1981 gagcggccgg ggaataactc ggtcgagatc acaagggcga aactacctgc gcaacagacc
     2041 attgtcgctc tggaatatgc gcggtagcga gacgctgacc gagaactcgt tgtacaatgc
     2101 tgaccgagaa ctcgttgtac aatgttgaag taacgaaccg ccggcttggc acgtttccgt
     2161 ggtccaggaa tggttaatgt cgaccgttcc gcggatcact cagacaggcg tagattcaca
     2221 gtatccgtca ttaagggagg cagaacgaat ctgaatcaaa ccttgcaaga ccttgggatc
     2281 gaccgtttgg acgtccagac aagacttgag ctaatcggtc tcatctggga cagcatcgac
     2341 gaaaacgagg aatccattgc aacgccggaa tggcaccttc gcgagttgga gctgcggcgg
     2401 gaagcggcgg atgccaaccc ggatgcagcg gttccttggg aattagttaa atctcgtttc
     2461 gagagagaac aataagcgtg cctgttgtcc tccgcctgtc ccccgacgcc acttctcggg
     2521 tcgtcgaatt cgacatcgaa cttcttgtgg gcagccccat cggccaagca ccacggacag
     2581 atctccccac ggaggttgtc gtcgctacca caggccggcc ctgtgtggat gaatccacga
     2641 gactgcctgc acactctcct caactcgcca tttcactaca gctcgcgcag cgccgaaatc
     2701 acatcgagcc gcacggcacg cagcgccggg aacatgccgc cgaaaacgcc catggccagg
     2761 gtcatgacca gggcgacagt catgaccagc ggtccgaagc ggaaggcgac ggtgaccgag
     2821 gcaaagctat tgaagtcgct cgtctcgtag gtcaaggcgt tgagtggaaa cgtcgccagc
     2881 aggccgatcg cgcccccgag cgcgcagagg agaagcgact cacccaggaa gctgatcaga
     2941 atgtcgagct gagagaaccc cagcgcccgc atggtgccga tctcgcgggt acgtgcgctg
     3001 acggcggcga acatggtatt ggccgaagaa aacatggctc cgaaggtcag gagcacggcg
     3061 atcaacgtgc cggcaccctt caagaatatg cctgaacggc tctgggtctc gaaataaagc
     3121 ggttccggga tcgccgctaa ccggaagcga ttctcgtcgt cgattgtttg cttgatcttg
     3181 ttacaatctg ccgagctggc cgcccggagc tgaacacacg agaccgaccc atcgcgaccg
     3241 gtgttctttt cgacgtcttt gagatcggcc cagatctcgc tctctgccgc gctgccgccg
     3301 gccgtgaaga tgccaacgac gtcatagact tccttggagc cgaacacgag tcggccgccg
     3361 acctgcgctc ctttgaaacg cgccgccatt cgtttactga cgatgcactc tcctttcccg
     3421 gcgacaaagt ccctgccggc gacgatcgtg aagctgggcc gaagcctcct ggaagccggt
     3481 tgaactccgc ggatgatgat gttggtcttc gtcccgttct cacgctccgc gatcgggata
     3541 ttcaaaagct ccttggcggc gatcggttgg ccttctcgat cgcgcaatat tcccggaaga
     3601 atcagaatct cgtcggcctt gctcgtttcg aatccgcctg ttgtttcggt cgatgaaccc
     3661 ttgcgcatca cgatcaagtc gagggggtcg cccgagatac tcaggctgtg ctccaagccc
     3721 tcgacgagac cgaacagaat acacgacgac cacacaacca aaccggtgcc catgacggtc
     3781 ataagcgtcg tcttccatcg cacacgcaaa ttgcggacgt tgtacttgag cggaatcatg
     3841 ggcgcctcag atgaccctcc tcaacccgtc aattaccgag agattggccg ctcgaacggc
     3901 cggataaaga ccactggcga gaccgatgaa aacagaaaca gccaggccct ggagcgcgat
     3961 gttccaggga acgtagtaga acggaagaaa accgcctgtg aatcgcgaga gatcgaccca
     4021 atcgcagaaa gccttcactc ccagagaacc gatcgcgcca ccgaaaccag cgatcaggac
     4081 cgcctcggtc aagaccatga acaggatcaa tcgcctggta aagccaatcg ccttcaagac
     4141 agccacttcg ctggttcgct cgcgcatcga catggccatg gaattgcttg cgacacagag
     4201 gagcgagaag atcacagcca ggccgatgat gcggatcatt ccctttaagt ctccgagcat
     4261 ctcctcgaac atcttgccga aggcttcctc cgtctgagtt cgcatcggaa aatcgctgtt
     4321 ccggtagctc tcatcaatgg tcttgcataa caggggcata tcatcggcgg tcttgcattt
     4381 gataaagatc atgcccgcgt tgcccgagcg ctgcccactg ccggttgtgg ccgaggtcga
     4441 gttggacagt ctgcgcttca gcagctcgtc gagatactcg aagcgcacca tacacatccg
     4501 gaggtcgcgg ttggacggcc cgtcgtagat gccctttacc gtcaggtcga ggtcgacggg
     4561 gtatgcatcg ccttttaggg gcagcttcga gcccacagtg agctttttgt cctcggcgag
     4621 cttgcggccg atcacgcagc cgtccctgct ggcctgaaat gacttgagct ggtcaggggg
     4681 tatcgtcagc tcgtccatga ccttgaatac cgtgtcggga tcgacgccga actgcgcaaa
     4741 cggcataatt tcatcttgat atttggcgcc gatccagcta aacgggctcg ccgcgacgac
     4801 accatcgagt tgagcaatct ccttgacgcg cgtgatcggc agcacgccgg cgaagccatt
     4861 ggcgttgagc gaggcgatcc ggttgaagat ccttgtcgac gaatttgcct cgtcgctgat
     4921 cgcgaaaaac gacagcagga tcatcatcaa agaaaggcag atactcatcg aggcgatcgt
     4981 caggatcgac cgtacggggt tgcggcgcgc gtttcgcaag atataggtga ggtatttcat
     5041 gaggcggccg aaatcgggct tccgggaacg gtttcattga cgagccggcc cttgtccagg
     5101 tacagcgtcc gcgtggcgcg ctgagcggcc aacgggtcgt gcgtgaccat cacgatcgtt
     5161 ttctgaaagt cccgattgag ttgttccaag aggtcgagaa tctcgccgcc cgtggtacgg
     5221 tccaggtcac cggtcggctc gtcagcaact aacaggtaag ggtcagtgat gattgcccgc
     5281 gcgatggcca cgcgctgctc ttggccgccg gagagttgac gtgggtaatg atgctcgcgg
     5341 ccggtgaggc caacgactcg taacgccgtc atgacatttt cgcgccgctt gcgtcgcgac
     5401 aagtgagtga gcaggagtgg cagttcgatg ttctcaaacg cggtcaaaac cgggatcagg
     5461 ttgtaggtct ggaagatgaa cccgacgttg ttggcgcgcc agcgtgtgat ctcggcctcg
     5521 gagagatcgc cgaggtcctg gccgtgcacc atcacctcgc cgctcgtggc ccggtcaagc
     5581 cctgcgatca gattcaggag cgtggtcttt cccgagcccg agggccccat cagcgccaga
     5641 aactcacctt ctccgacgga aacgtcgagg ttgaccagaa ctggaatccg gaactcgtca
     5701 cgccggtatt ccttgtaaac accgcgcaag acgatcgagg acatttcggc cattgaccat
     5761 gaacctttcc cggtaggggc gtacgccttg actagtcgat tcgaacgacg tcgaattcgc
     5821 ggagagtttt catcgggcca agaacgaccg cgtcgttcaa tttaagtccc gactcgaccc
     5881 gcgcccaatc gtcggtcgtt gatactacct cgacccgata catgcgaaga gtattgtcat
     5941 cgcggatgac ccaaacgaag tcgcgtccgt cctcctggaa cacggcggat ttgggcacga
     6001 aaagaaaggg gcggtccgcg tccgggttta tgaccgactc gctacgcagg aaggatacct
     6061 tggcggccag ctcggggaaa agccgatcgt cggggtcgag gatctcaacc ttgaccttga
     6121 cgaccgcatt ggcccgatcg ctcatcggca gcacctgcct gagccggcct cggtagcgct
     6181 tcgatggaat cgcgctgacg gacactacgg ccggctggcc cagtgtgatc cgcgatagca
     6241 agccctcttc gattttggtc tcgacatcca tcttttcgag gttggcgatc gtcacgaccg
     6301 ccgtccggcc cagcgacgag ctcatcgccg tcggcgagat gatttcaccg acctcgccct
     6361 gcttttcgac gaccgtaccg tcgaacgggg cataaagatg catcgtgaca atagtggcct
     6421 tgatctcctc gacgttggcc ttcatcagtt tgacagcggc ttcgagggcc aagacccgcg
     6481 cggccgcctt cttgtggcct gcctgagctt tctcagattc ctcgggagtg atgctccgct
     6541 gcgcgaagag ccggctcgct cgccggtcct ctcgctcttt ctcccacagg tcggcccgcg
     6601 cttcctccag ctcggcttgg gtgcgctggg cttgcgcttc tcttgaggcc agcatcgcct
     6661 tcaaatcgtt gtgctcgatg acggccagcg tgtcgccttt cttgaccttc atcccttctt
     6721 cgacggccat ttgctcgacc cggccagcaa ttttcgtccc aatcatcgct tgatagcgcg
     6781 atttcagata tccgttggcg tcaagcaatg ttgctgcttc cgccgctgtt ttcgatacca
     6841 ccagtccctt cttcaccaag ggacgagacc gcatcgtatc gtactggata taaccataaa
     6901 cgccgccagc cgccaaaagt cccagcggga tcagccaaag catccaggac agcagccgca
     6961 gcccactacc gccgcggcgg cgtgatgatt cgcgataccc gttgcgcccg gattttatcg
     7021 ggttgggccg atcaatcttg agcgatgcga gttcgtcgcg gagcgtaccc gccatgagca
     7081 acctcatacg gaatgaacgt gatcaaaacc cgtcagcagg aatgcaatga ccgcgcatcc
     7141 cgcgggtgtc attaatatta atgacttcaa tcagccgtgt aaatcgcgtc gggcccgttg
     7201 ctcagggaga gccgccgacg gaatgcgccg gctcggccgt cttgccgccc ggagggtcaa
     7261 ccgtgagcgt gtagatcttg ctgcgctcat tcaagcggct gaatttgccg tgaaagacat
     7321 cgacttttcc ggcatgcttc actcggacct gccagtgggt cggccgggga actccgccga
     7381 agccgcccca gtaaaccaca aaccatttgt aggttccggg gggacctggc cccttgacct
     7441 tttcagatcc cggtccctgc gactcgacca tccagtagat gttttcggga ccgaagccct
     7501 tggtattgtc gacgtcgagc tcgccccctt gccgaccctt tggttcttcc cagtagattt
     7561 ccttgccccc tggctcgagg acatggaggt caaggtcgac cccgtcggag ttccagatca
     7621 aggtgaactg agggtcgccg accttgaccg agacgccccg gatcagctcg cccccttcgc
     7681 cgaaccgagc aaggccaaac ccatcgccat ccccggctcc cgggttatcg ttggccttac
     7741 caccgccgcc actgggattc ttggcgagcc gtaaccggac ctctggttcc ggaggtgcaa
     7801 gctcaagctg gccgagcgcg ggagaatcta acccgagcac tttctcgaca agcggagctg
     7861 ccgacaaggt cagcggcgta ctcagtccgc cggtgagcat ctcgccttcg ggcgcgcctt
     7921 taagggagcc ggccagccgg gaatcgaacg tgattgaccc cttcgtccga ggggcaaaat
     7981 accagcacgc gaggactaca agcaaaaccg cgtgcagcgt catcgagcct gcccaaccct
     8041 tgagcgcctc gggagtccac cattcagcct ccagtggaga atcgggctcg ccgtcaggaa
     8101 cgatgcccgg ggcgggcaca ggggggcggg cgatgggaac ttgcgagctc cctttggtac
     8161 tcgccgatcg aagtcgtgac gggctggccg acatcgtcgc acccagtttc cgcatcgaca
     8221 ggacacgctt cagaacgccg acgccacgtt cggcctggtg atccgaatcg cctgctgatt
     8281 gagacgtacc aattcgctcg gactactgag tttaccatgg gccgtcctcg acccgaaagc
     8341 tcaaacgggt gcgccctttg acgacctcga aaacaaacac ccggatctcc cggaaagtca
     8401 cgcacgagac gattgaacaa ccggttcgct cgccataaac ttaggaagct tcaggcccag
     8461 atgacgggag acgatgatgg attcgcacga gctcaaagaa ctgctcgacg ccgttcgaga
     8521 cggtcgcgta ccgcccggag tcgcagccga gcggatccag gtacagccgt atcaggacgc
     8581 gggagggttc gccaaggtcg atttgcaccg ccggctgcgg tgtggttttc ccgaggtcgt
     8641 ctttggacag gggaaaacag ccgcacagat cgaggcaatt ctcaagactt tattggaaca
     8701 cgagcaaggg ggcttggtga cgcgcctgga tccacaggcc gcccctcatc tgctggcggc
     8761 cttcccgcac ggcgagcata attctctcgg gagaacgttt cgcgtccggt cttccgaaga
     8821 tgagggcccg aagctgggcc gggttgtgat cgtcacggcc ggcaccagcg acctaccagt
     8881 tgccgaggaa gcacgagtga ccgccgaggc ctggaactgc gatgtgacgc tcgtcgccga
     8941 cgtcggcgtg gcggggttac atcgcctgct gcatcagctt cctcaattga agacggccga
     9001 cgcactcgtc gtcgttgccg gcatggaagg cgcattgccg agcgtggtgg gaggcctggt
     9061 cgaatgcccc gtcatcgccg tgccaaccag catcggttac ggagcacatt ttcagggagt
     9121 ggcggccttg ctggggatgc tcaatagttg cgcctctaac gtcgtcgtcg tgaatatcga
     9181 cgccgggttt agcggcggcc acgttgcgag cctgattgcc cgccgtatgg gccaggcccg
     9241 gcaagccgca gccaggccgc tctaacagcc gcggcggggc cgcgccggag tcgtccggag
     9301 actcagacca ggctattctc aatcttcgat acacgccacc gcaggagcga cccgttcatg
     9361 acattggaaa aggttgacac ggtccccaac ctgcctgccc gaccgcgcga tagccacaag
     9421 gggcgctttg gttccgtact gatagtggca gggtcgcgag ggatggcagg cgctgccgcc
     9481 ctttgcggcg gcgccgctct ccgatccggc gcggggttgg tcagggtcgc cacgcccagc
     9541 gaaatccagc cgacagtgtc gagcttcgag ccttcctata tgacctaccc cctaccctgc
     9601 gacgaaaacg gtctgattcg gtttggaccc gcgcggccgg tcctcgaacg gctgatcggg
     9661 cacgccgacg tcgtcgccgt cggcccaggg cttggtcagt cagacgatat ccaacagctc
     9721 gtgggtttct tgatcgcatc cgcccaaaaa cccctcgtca tcgacgccga cggtttgaat
     9781 gccctggtcg gccaaaccga tctcctgtcc cagcgcaagt gtccggtcgt gttgacaccg
     9841 catcccggcg aatttgctcg gctgactggc tcgacaatca ccgaggtcca gtcagaccgt
     9901 gtccagcggg ccgccgcact ggcgacgctc tcaagcgaac tcgtcgtggt cttgaaaggg
     9961 gccggcactg tcgtgaccga cggcaataaa tattacatca acacgactgg caaccccggc
    10021 atggccaccg gcggaacagg cgacgtactg accggtgtga tcgccgctct cctcgcccag
    10081 aaactacccc cgttcgacgc gacccagctc ggtgttttca tccatgggct ctcaggcgac
    10141 atcgcccgcg atcagagcgg tgaaatcggt ttgatcgccg gcgacttggt tgacgcgctg
    10201 cccgatgcat ttgtacacgc gatgcctgat ccagacccga tctcgatttc caactaaacc
    10261 tcgacccatc agtattatgg ccggtggttg gctcatatcg tgatggcaaa tcatcgatcg
    10321 ctttccagaa agtggcggcc actggactat gccatcggcg agctccggcg tagaatattt
    10381 cttgatcacg gaccgatagc caggtccgtc agcctgcgat gagcggagaa acgtcgcggt
    10441 tcccctcgga acgagcgaag gaggttggag tgtcaacacg cgagaacccg tcgtcccccg
    10501 cccctgcctg gacctatcgc atggaggttt caccgacggg tcaaagcccg acagccgcga
    10561 tcgccaaccc caatgatccc atcctcttgc tccatttgct ggccaacctc cagagccaga
    10621 cgttggaggt ccaacgccag atgcttgaga tgcagcgcca gcagctcgag atttctcgcg
    10681 agctgatcca ggtcaatcgc gagcagcgag cgcgtcaggg ggccgagctg gagcggtggc
    10741 aggccggtca cgagccgatc ctcgagacct gccgtgagac gctaggcaag ctggaacagg
    10801 ttcatgcctc gttgatgagc gagctcgcca actacgtgga tgataaccac gaaaacctgc
    10861 tcgagggaga cttttcgctc agtgatttcg tcgaccgctt tgggcctcgt ctggcccatt
    10921 taaacacgat gttggccgtg ctccgcccgc tggccgccgc caagatcaag accgaaagct
    10981 gaaagaatct ctccgcccgg atcagttcgt tcgcgatgaa aactggtata tgcggatggt
    11041 tgcgacggcg acggtgagaa tcgacgcgag ataggttcgc aggttctaga gttacgtcag
    11101 tccatgtgcg caattccccg aagcggcgag gcagggatcg ggcaacgtgg ggcaagaagc
    11161 gaaatgacct caggagcatg cgaagagaag aatgctcctg ggtcggctgg attattgtgc
    11221 ttaggtgtgg cgcggactgg aaacctcgat gcctattcaa tagcgacggg tgtcgtaacc
    11281 gccgctccgg ccacggccac caccgccgcc ggcaccacca ccgccaccag caccaccgcc
    11341 tcctccaccg cgccatccac caccgcctcc accaccaccg ccggttcgcg gctcccgcgg
    11401 tcgggcttcg ttgactgtca ggcgacgacc gtcgtgttcc tggtcattca gggcctcgat
    11461 tgccgcccgc gcttcgttgt cgctgctcat ttcgacgaac ccgaagccct tgctcctacc
    11521 tgtgtcgcgg tcggagataa cctccgcgct ctcaacactg ccgaattgtg aaaacagttg
    11581 ttccaggtcg gaactgctca cccggtaagt gaggttgccg acatacagtt tcttacccac
    11641 catcaatctc ctgagaccag ggtgtcacga cccattggtc actttccggg tcgtaccgaa
    11701 tggaccgacc gggcggccca atggatggaa aggcgagaga ggcaaacgaa cagattccgc
    11761 agggatctaa agcgcgcact cactcatcta ccaaccgctc cacagtataa taaagatcag
    11821 attatcacgg gtagaccgaa ttctgtgaaa tcattgtatt tttcgcccaa tgcgacgaac
    11881 tgcctcaagc ggggatttac agaggcatca cgacagttcg cggagtggga ttgatcgtgc
    11941 gttcgatgcc atagacgagc gccggatgtc ccatgatttc tgtcgaccat tctttgcgca
    12001 ttccgagccg cagggcgacg gcgatcgaag gaccgttttc cggccggatc aagctgatca
    12061 agcggttttg gcccatacgg gagaaggcgt cggccatgac ggtttgcgcc gcctcggtgg
    12121 catagccgcg cccccaatac tcgcgcctta gcgcccagcc gatctccatc ccgggccagc
    12181 cttccgggcg ccagcatccc actcggcccg cgaggattcc ggtcgctcgt tcctcgacag
    12241 cccaaaagcc gaacccgcgc aattgccagt gccctaaaat cagagccata ttgcgccagg
    12301 cctcggcacg cgtcagtgga tagccgtctc ccaggtagcg catgacgtcg ggatcaccgc
    12361 acattgcggc ataggcatcg aggtctgact cgcggaacat tctcaaaatc aagcgattgg
    12421 agccaagcgt gagcatgggc gttgggacgg attagagtct tgcctcgcct cgacatctcg
    12481 ctgttgccgt cggcggggct cgcttacgcc gtctcgggct gactttttcg tagcggccac
    12541 gggcggggga acttggtctt caagtctttc catatcgctt ctcgcacccg agttccactg
    12601 tccctgacaa ttccggagag caggcgagcc gcttcggccg tggcggctcg gcggacctga
    12661 gtacgcggct ccgccaccag cgatccgcaa tagtccttgg cgatgatccc cactcgcagt
    12721 gtgaggaagg cgttcgcgct gccgcttagc agcgagttta ccatgatcga ggtcatgatc
    12781 tgaaagcctg gaatcgcgcc cccgagcgca cccacagtgc cagccacgac gggctggatc
    12841 atctggtgca actcgagatc atcgagctct cctgccacga acgatgtggc cgcgacgttg
    12901 gcatagagat gggcaagttc tctcagggag ggccgctgat aatagaggcg cgcgacgcga
    12961 tagaccatgc gtgattgggc ggctagcacc agcactgcgt ccagccgccc gctttgcgaa
    13021 accgccgtgg tcaagaagac cgtggtcgcc atttgcttga cgatggcgtt ggcatcatcg
    13081 tcaaggactt gaagcgcatc ttcgacgcca gcgcggtcga agggcgcgat cgaggcacgc
    13141 tggacgcgcg ggttgaccgc caagcgctga cccagttgtt tcaagtgttc gtcgaattcc
    13201 ggtccagact cagtcgcggg cggcacaagc gcgcggggca tacgcatgac catcacgacc
    13261 ggaacgccga tcaatccgcc ataggcaatc aggagtgtcc agagcgtgac actccccagg
    13321 gccggatgga cctccttggc gagctgcaca acccctgcag tctggttgat gaccaccacg
    13381 acgaagctga gcaccaccgt ggcgctcgcc agcgctgcca aggtcttgac cgtcttattc
    13441 atggacgtcg atcccgatgc cgccatcacg gactcccaaa acgccacaac cgtccatttc
    13501 gtattcgtgc cgcgggtgcg aaatattcga acaacaccct cgggtcattg gcgcgacgga
    13561 tccgttcaag gagcggttgg gccatgatct tacccgaaga agggcggcaa agcgcgaccg
    13621 gatcgcaaaa aaaatcaact cgaccatcat gcttcaagtc gtgtaatcgg cattgagccg
    13681 aatgtattcg gcggtaaggt cgcatgtcca gaagcggacg cattctttac ccgacttgaa
    13741 acgcagccga agatcgacat tgcgctcggt tcttagcgat cgtgacaatc ccgcgtcatc
    13801 gaacggaatc gggctgccgt ctttataaac ggcgaccgag ttgatccaca gcgagagttc
    13861 ctcctcctga aacgcaatgc ccgcgtagcc ggccgcggag acaatgcggc cccagttggg
    13921 gtcggcccca tgaatggccg tcttgacgag cggactgtcg gcgacggccc gtgcgatcgc
    13981 gcgagcgcct tcaaaagtct cgcatccttc cacgtcgatc gtgatgaagt gggtcgctcc
    14041 ttcgccgtca tcggcgatcg cgcgggccag cgtttcgcaa gtcgaccgca ccatcgcggc
    14101 gaaggccgcc agcgattcgc ctcgcaggac ctgttcggtc gtggccgtgc tcgagagcaa
    14161 gaggaccgtg tcattggtac tggcatgccc ctcgaccgac aggcaattga atgagtgctc
    14221 gacggcatcg gcgagtatgt cctggagctc gttggcccaa actggggcgt cggtgagcaa
    14281 gaagccgagc atcgtggcca tgcgcggccc gatcatggcg gcacccttgg cgatccccaa
    14341 aagcgcgacc tctcgcccat caattgtctc gcgcagcgag acgatctttg gtcgcgtgtc
    14401 ggtcgtgatg atcgactcgg cgacagtttg aaatgcctcc gggcgatcgg aaagtccggc
    14461 gacggccttt tcgatccctg cctcgatctt atccatcgcg agttggtggc caatgatgcc
    14521 agtcgatgcg accaggactt cctcaggctg acaacccagg agcgttgcga ctcgctcggc
    14581 tgtgcgccga gcgttggcta atccctgctc gccggtggcg gcattggcat tgcctgcgtt
    14641 gatcgcgatg gcgcgaatgc tgtcggcggg gagcagctcc cggcaccact tcaccggggc
    14701 tgcacagact cggttggtcg tgaacgtacc ggccgcagca cacggctgat ccgccgccag
    14761 caccgccaga tcgagcgagc ccgatgcctt caatcccgct ttgactccac tcgcgcggaa
    14821 gccgcgtggg atggccaatt gcg
//
LOCUS       DATAQL010001081         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3317905, whole genome shotgun sequence.
ACCESSION   DATAQL010001081 DATAQL010000000
VERSION     DATAQL010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3317905"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(452..1888)
                     /locus_tag="VHS97_18660"
     CDS             complement(452..1888)
                     /locus_tag="VHS97_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3450283.1"
                     /translation="MRRALFFLFAFLPLASGGQTAQAADRPNILWLVAEDFGPELGCF
                     GTNQVWSPNLDRLAAQGVRYTHAYTTAPVCSASRSAFMTGMYQTTIGAHNHRSHRDDG
                     YHLPTGVRVISDRMRAAGYFTANVRWLPESFGFSGTGKTDWNFTYDGEPFDSDRWTDL
                     KTHRPFFAQINFQETHRKFNAPKKADPAKVVIPPYYPDHPVTRQDWAQYLDAASELDR
                     KVGVILRQLEVEGLAESTVVVFMGDHGQAHVRGKQFCYEEGLHIPLLIRWPKAATPPR
                     SFQPGAVDDRLIEAIDLAPSFLSIAGATKPEGMQGENFLGDHAASPRQYVFGARDRCD
                     ETVFRLRTLRDARYRYIRNFTPERPFLQPNEYKERSYPVWNLLKQLDAQGKLTPVQKV
                     LTAPTMPAEELYDLDNDPHEIHNLATSDKPDHQVVLKRLREALERWIEESDDQGRVFE
                     TASVVAAKGATRPGTDPNSGMIPTARKK"
     gene            complement(2014..>2547)
                     /locus_tag="VHS97_18665"
     CDS             complement(2014..>2547)
                     /locus_tag="VHS97_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3450284.1"
                     /translation="VRTTLLGDLPAISIAFSPDGRSLASGARDKKTVKLWDLETGRSR
                     ILFRDASGPISSVAFSSDGSLLAAACSIDQLVRLWDVNSGRLWRQFEGHRGGTNSVKF
                     SPDGETLTTSGSDGFVKLWRVATGEQLASLDGQSLWLPNLAFSADGRTLFATGSDNNI
                     RLWDMDDVFPDKCSLPE"
BASE COUNT          473 a          739 c          814 g          521 t
ORIGIN      
        1 aggctgcgcc tgctcaccaa gccattcgcc cgatatggaa agccaggatt agacgtgcag
       61 gacggcgcgc tgttctgttt cgcccacgga actgaccccg aggtcctatt aatgctcgag
      121 tcgcggcctg gcaaagatgg ccccgaatgg caatatgcct tcgcgccaat gacagccttc
      181 gccgtcaaca gtcgtggaaa ggcaaggagg tctggagctt gcctccgcag gccaacggct
      241 cggctcggga cccgagcaat acatttcaca tacgtcgcgt ctcagtcaga tctgatcgta
      301 attgaatgtc attcaccatc gataagtgtt ccgaagcaaa atccggcctc gtcgagggcg
      361 cgatcttcat cttcgccacc attaccaact caagatggtg ctgctgatcg aggggcggat
      421 ttgggtgagg ctgtcatcac cggagtctcg gtcatttctt cctggccgtg ggaatcattc
      481 ccgagttcgg atctgtgccc ggccgggtgg cccccttggc ggcgacgaca gaggcggtct
      541 cgaagacccg gccctgatca tccgactctt cgatccagcg ctcaagcgcc tcgcggaggc
      601 gcttcagcac aacctggtga tctggcttgt cggaggtggc gagattgtgg atctcgtggg
      661 ggtcgttgtc cagatcgtac aactcctccg cgggcatcgt cggcgcggtc agaaccttct
      721 gcacgggcgt tagcttacct tgggcatcga gctgtttcag caggttccag accgggtagc
      781 tgcgttcctt gtattcgttg ggctggagga acggccgttc gggcgtgaag tttctgatgt
      841 agcggtatcg cgcgtcgcgg agcgtgcgga gtcgaaagac tgtctcgtcg catcggtcgc
      901 gtgcgccgaa gacatattgc cgtggcgatg ccgcatgatc gccgaggaag ttttctcctt
      961 gcatcccctc cggtttggtc gcgccggcga tgctcaggaa gctcggcgcg aggtcgatgg
     1021 cctcgatcag gcggtcgtct actgcgcctg gctggaagga tctgggcggt gtggcggcct
     1081 tcggccagcg aatcaagaga ggaatatgca gtccctcctc gtaacagaac tgcttcccgc
     1141 gcacgtgggc ctgaccgtgg tctcccatga acacgacgac cgtgctttcg gccagcccct
     1201 ccacttcgag ctgccgcaag atcacgccga ccttgcggtc gagttcgctg gccgcatcca
     1261 ggtactgagc ccagtcctgc cgcgtaacgg gatggtccgg atagtaaggc gggatcacaa
     1321 ccttggcagg gtctgccttt ttgggggcgt tgaacttccg gtgcgtttcc tggaagttga
     1381 tctgggcgaa gaagggacgg tgtgtcttca agtccgtcca ccggtccgag tcgaacggtt
     1441 ctccgtcgta ggtgaagttc cagtcggtct tgccggtacc ggagaacccg aacgactcgg
     1501 gaagccagcg aacattggcc gtgaagtagc cggcggcgcg catccggtcg gagatcaccc
     1561 gcaccccggt cggcaaatgg tagccgtcat cgcggtgcga tcggtggttg tgggcgccga
     1621 tcgtggtctg gtacatgcca gtcatgaacg ccgagcgact ggcggaacag accggcgcgg
     1681 tcgtgtaggc gtgggtgtag cgcacccctt gagcggcaag acggtcgagg tttgggctcc
     1741 agacctggtt cgtgccgaaa caaccgagct cgggtccgaa atcctctgcg acgagccaaa
     1801 ggatgttcgg tcggtccgcc gcctgcgcgg tctggccacc gctggcgagc ggaaggaacg
     1861 cgaagagaaa gaacaacgcg cgtctcatgc cagtgctcct cggcgtaggt cgtgtcactc
     1921 gcccttggga cttggggacg cgggtcccaa gacggtggtc cggctttccg cgagtgtagc
     1981 atcctgaccg gatatgtgag atgagcgact tggctattct ggcagactgc atttgtctgg
     2041 gaagacgtcg tccatgtccc agagccggat attgttgtcg ctgccggttg caaagagcgt
     2101 tcggccatcg gcagagaatg ccaggttggg cagccacaag gattgaccat ccagactggc
     2161 gagctgctcg ccggtggcga ctctccaaag cttgacgaac ccgtcactgc ccgatgtggt
     2221 caaagtctct ccgtcgggtg aaaacttgac cgagttggtg ccgcctctgt gaccctcaaa
     2281 ctgacgccac aatcgacccg aattaacgtc ccagagccgg acgagctgat cgatcgaaca
     2341 agccgcggcc agaaggctcc cgtcagacga aaaagcgacc gagctgatcg ggccactagc
     2401 gtccctgaag agtatccggc tgcggccggt ttcgagatcc cagagcttaa cggttttctt
     2461 gtctcttgca cctgacgcga gagaccgacc gtcgggcgag aatgcgatgc ttatggctgg
     2521 aaggtcaccc aagagggtcg tgcgtac
//
LOCUS       DATAQL010001082         5319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3163220, whole genome shotgun sequence.
ACCESSION   DATAQL010001082 DATAQL010000000
VERSION     DATAQL010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3163220"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /gene="rpoB"
                     /locus_tag="VHS97_18670"
     CDS             <1..445
                     /gene="rpoB"
                     /locus_tag="VHS97_18670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244121.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX3450285.1"
                     /translation="CLKDAGLPENGKAVLYDGRTGDKFDQRVTVGYIYMLKLHHLVDD
                     KIHARATGPYSLITQQPLGGKARFGGQRFGEMEVWALEAYGAAYILQELLTVKSDDVE
                     GRTKIYESMVKGENTLEAGTPASFDVLTNEIRGLGLNMQLEKKRV"
     gene            538..903
                     /locus_tag="VHS97_18675"
     CDS             538..903
                     /locus_tag="VHS97_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450286.1"
                     /translation="MPKTSTISPAELETLDRFYREGGLRVMASPHVHYDEPTCPHPGC
                     SHHLEWIDFKLELHGDPEGIYKPLVRAWWEGSGFVGRCPACKEWIRFTTIKMEAVDDD
                     IAAHSLQLPDNWHAVAQFA"
     gene            1539..>5319
                     /gene="rpoC"
                     /locus_tag="VHS97_18680"
     CDS             1539..>5319
                     /gene="rpoC"
                     /locus_tag="VHS97_18680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX3450287.1"
                     /translation="MGESAYDRINDYGAVKIGLASPYDIRSWSFGEVKKPETINYRTY
                     RPEKDGLFCERIFGPEKDWECACGKYRGMKYKGMICDRCGVKVTHSRVRRKRMGHIEL
                     AAPVVHIWFFKAMPSRLGTLLDMRTTSLERIIYFQDYVVVDPGDTPLKERQLMTEEEF
                     RKAKELYKPGGQYNEEFQADMGAEAVRKLLMRLDLVALSKQLRQELVETSSKQKIKDL
                     TKRLKVVEALRDSDNRPEWMVLECIPVIPPDLRPLVLLDSGNFATSDLNDLYRRIINR
                     NNRLKKLVDLNAPEVIIRNEKRMLQQAVDALFDNNRCKRPVLGSSNRPLKSLTDMIKG
                     KQGRFRENLLGKRVDYSARSVIVVGPDLKLHQCGLPKKIALELFQPFIIRRLKELGHA
                     DTIKSAKKMLERRTDEVWDILEEVIRNHPVLLNRAPTLHRMGIQAFEPVLVEGNAIRI
                     HPLVCKGFNADFDGDQMAVHLPLSIEAQVEAMTLMMATNNIFSPANGNPIISPTQDIV
                     MGAYYLTVSRPSEKGEGMTFSTPNEVFLAHSQGKVGVHAMINLRLPPNRRLRGDGEKE
                     FKPGMIVKTTVGRVVFNDILHSRMPFYNLTLGQKQLQGIIADCYQILGRRETISLLDR
                     MKDVGFRESTRSGLSFATDDLKTPQSKDAIIAEAEKEVTKQNRFYQRGIITDQERYNK
                     VLDAWTHARERITHEMMEALKNDSREGEVYLNPIYLMAESGARGGVEQIRQLAGMRGL
                     MAKPSGKIIETPIKANFREGLSVLEYFSSTHGARKGLADTALKTADSGYLTRKLADVA
                     QNVVITMEDCGTSQGITKGVIYKGEKVEVSLVQSIRGRVSRVNIVDPLTEEVVVKDSE
                     MITLAAARKLEDMQIEKIQVRSPMTCEASLGVCRCCYGMDLATGQLVEVGMAVGIIGA
                     QSIGEPGTQLTMRTFHIGGVATRGVEEKDVKSKREGKVKFVGLNIVINDEGKKIALSR
                     NGEIQILDPKGRELEKYDVPDGAIMMVEDGQTINRGSMLCEWDPHNIPILAEVGGKVR
                     FEDIVEGETMKIEIDPSGHVRRTIIEHKGDLHPQVIIVDAEGKTLDYKYIPERASIEV
                     NDGQMISAGTLLAKTPREVGGTQDITGGLPRVTELFEARRPKEPAVIAEIDGRVELLD
                     EKRRGKRTIIVRNESGIEREHLVPHGKYLRVHGGDRVRAGDPLVEGPLVPHDILRISG
                     EEAVQRYLLREIQNVYRSQRVEIDDKHLEIIIAQMLRKVRVDSVGDTPLLPGSVID"
BASE COUNT         1190 a         1527 c         1615 g          987 t
ORIGIN      
        1 gtgcttgaaa gacgccggct tgcccgaaaa cggcaaggcg gtcctctacg acggccgaac
       61 cggcgacaag ttcgaccagc gagtcacggt tggatatatt tatatgctga aacttcacca
      121 cttggttgac gacaagattc acgcccgcgc gaccggtccc tactcgctca tcacccagca
      181 gcccttgggt ggtaaggcgc ggttcggcgg ccagcggttt ggcgagatgg aagtctgggc
      241 tctcgaggcc tacggggcag cctatatcct ccaagagctg ctcacggtga aatccgacga
      301 tgtggagggt cgcaccaaga tctacgagag catggtcaag ggcgaaaaca cgctggaagc
      361 cggaacgccg gccagcttcg acgtcttgac caatgagatt cgtggtctgg gcctcaacat
      421 gcagctcgag aagaagcggg tctgactatc ggtatgcgaa agccggtctt ctctcttgta
      481 tgatgaaaga gatcaagacc ggagtgcgca ccgacctcag gcccgacgga gttgaacatg
      541 cccaaaacat cgacgatttc gcccgctgag ttggaaacac tggatcgctt ctaccgcgaa
      601 ggtggactcc gcgtcatggc gtcgccccac gtccattacg atgaacccac gtgtccccat
      661 ccgggctgct ctcatcattt ggagtggatc gacttcaaac tcgagctcca cggcgacccg
      721 gaaggaatct ataagccgct ggtccgcgcg tggtgggagg gaagcggttt cgtcggccgt
      781 tgcccagcct gcaaggaatg gattcgtttt acaactatca agatggaggc cgttgacgac
      841 gacattgcgg cacactctct ccagcttccc gacaactggc acgcggtggc tcagtttgcg
      901 tgaacggcgt ggctcttcgg ctgcgacatt ggaaagcggc accacgcttc tcactgccgc
      961 gatctatggt cttccgaggc tcaatatttc ctgtgccttt ggcctatttg gcgcgagcaa
     1021 atcttgactg cagtaacaaa agttcagcac gcggaaccga cgcccgcaaa aaaatcaaag
     1081 cgatcaagaa gatttttctt ggaaacggtt caaggattgc gtaggatcat aggatgcgac
     1141 tccaccgaac gccgattgcg cctcgacggg ggctacttgc tggccgccag tgtcgaagtt
     1201 tcgtgtgcaa atgttggagt ctccaatgac ccggtcaagg agcgagccgg ttgggtgcct
     1261 gtggtctggg acgaccgtat tggaccgcgg cagtcttcac ggcatggccg gaagacgggc
     1321 tcgcgggttt ttttgtttgg ttttgaatga ggcgatctga ggaccgatac cgatcgacct
     1381 caattcacga cgtgacctga cttgagctca cttgttttga gcaaacaacc cgggcggtcg
     1441 tccaccagcc ggcgtaatgc cattggccgc cgctttcacc caccaaccac cccgcttcgg
     1501 cacagccaag ggcgtgcaga ggagagtgtc tcgtgagcat gggcgaaagt gcctacgatc
     1561 ggattaatga ctacggtgcc gtgaagatcg ggcttgccag cccatacgac atccggagtt
     1621 ggtcgtttgg cgaagtcaag aagcccgaga cgattaatta ccggacctac cggccggaaa
     1681 aagacggtct gttttgcgag cggatcttcg ggccggagaa agactgggag tgcgcctgcg
     1741 gcaaataccg cgggatgaag tacaagggga tgatctgcga ccggtgcggc gtcaaggtga
     1801 cgcattcacg ggtccggcgc aagcggatgg gccacatcga gctcgccgcc ccggtcgtcc
     1861 acatctggtt cttcaaggcg atgccgagcc gattgggcac gcttctggac atgcgcacga
     1921 ccagcctcga gcggattatc tacttccagg actacgttgt cgtcgacccc ggcgacacgc
     1981 cgctcaaaga gcggcagctc atgaccgagg aagagttccg caaggccaaa gaactctaca
     2041 agccgggcgg ccagtataac gaggaattcc aggccgacat gggggccgag gcggtccgta
     2101 aactcttgat gcggctggac ctggtagccc tctccaagca gcttcgccag gagttggtcg
     2161 agacatccag caagcagaaa atcaaagact tgaccaagcg tctcaaggtc gttgaggcgc
     2221 tgagagacag cgacaatcgc cccgagtgga tggtacttga gtgcattccg gtgattccgc
     2281 cggatctccg gcccctggtt ttgctcgatt cgggcaattt cgccaccagc gacttgaatg
     2341 acctgtaccg gcgaatcatc aatcgcaaca accggctcaa gaagctggtc gacttgaacg
     2401 ctcctgaggt cattatccgc aacgaaaagc ggatgcttca gcaggccgtc gatgccttgt
     2461 tcgacaataa ccgctgcaag cgtcccgttc tgggaagctc taaccgtccc ttgaagtcgc
     2521 tgaccgacat gatcaaggga aagcagggcc gattccgcga aaatctgctg ggtaagcggg
     2581 tcgactattc ggcccggtcg gtgatcgtcg tcggacctga tttgaagttg catcagtgcg
     2641 ggctgcccaa gaagattgcc ctcgagttgt tccagccgtt tatcatccgc cgcttgaagg
     2701 agttaggcca cgccgacacg atcaagtccg cgaagaagat gctcgagcgc cgcaccgacg
     2761 aagtttggga cattcttgaa gaggtcatcc gcaatcaccc cgttctcttg aatcgtgcgc
     2821 ctactttgca ccgaatgggc attcaggcgt ttgagccggt gctggtcgag gggaacgcca
     2881 tccgaattca ccccctggta tgtaaggggt ttaatgccga cttcgacggc gatcagatgg
     2941 ccgtccacct gcctctgtcg attgaggccc aggtcgaggc catgacgctg atgatggcga
     3001 ccaacaacat cttcagcccg gccaacggca acccgatcat cagcccgacc caagacattg
     3061 tcatgggcgc gtattacctg acggtcagcc gcccgagcga aaagggagaa gggatgacct
     3121 tctcaacacc caacgaggtc ttcctggccc acagtcaggg caaggtcggc gtgcacgcga
     3181 tgatcaacct tcgcctgccg ccgaatcggc ggttgcgcgg tgacggcgag aaggagttca
     3241 agccggggat gatcgtgaag acgaccgtgg gacgggttgt cttcaacgat atccttcact
     3301 ccagaatgcc tttttataac ctcacgttgg gccagaagca gcttcagggc atcatcgccg
     3361 actgctacca aatcctgggg cgccgcgaga cgatctcgct tttggaccga atgaaagacg
     3421 tgggtttccg cgagtcgacg cgctccgggc tgtcgtttgc cacggacgac ctgaagacgc
     3481 cgcagtcgaa ggatgccatc atcgccgagg cggagaaaga agtaaccaag cagaaccggt
     3541 tctaccagcg cggaatcatc accgaccagg aacgatataa caaggtgctg gacgcgtgga
     3601 cgcacgcccg cgagcgcatc acgcatgaaa tgatggaagc cttgaaaaac gacagccgcg
     3661 agggcgaggt ctacttgaac ccgatctact tgatggccga gtcgggcgcc cgcggcggtg
     3721 tcgaacagat ccgccagctc gccggcatgc ggggcctgat ggccaagccg tcggggaaga
     3781 tcatcgagac cccgatcaag gccaatttcc gcgaagggct ctcggtgctc gagtatttca
     3841 gctcgaccca cggggcccgc aaggggttgg ccgacacggc cttgaagacg gccgatagcg
     3901 gctatttgac cagaaagctg gccgatgtcg cccaaaacgt cgtcatcacc atggaagatt
     3961 gcggcacctc gcagggcatc acgaaggggg tcatctacaa gggtgaaaag gtcgaggtca
     4021 gcctcgtgca atcgatccgc ggccgcgtca gccgggtcaa tatcgtcgac ccgctgacgg
     4081 aagaggtcgt ggtcaaggac agcgagatga tcaccctggc cgccgccagg aagctcgagg
     4141 acatgcagat cgagaagatc caggttcgca gcccgatgac ctgtgaagct tctctgggtg
     4201 tctgccggtg ctgctatgga atggacctgg ccacgggtca attggtcgaa gtgggcatgg
     4261 ccgtgggcat tatcggcgcg caatcgatcg gcgagccggg aacccagctc acgatgcgta
     4321 ctttccacat cggcggcgtg gccacccgcg gcgtcgagga aaaagacgtc aagagcaagc
     4381 gcgaaggtaa ggtcaagttc gtcggcctca acatcgtgat caacgacgaa gggaaaaaga
     4441 tcgctctgtc gcgtaacggc gaaattcaga ttctcgatcc caagggtcgc gagcttgaga
     4501 aatacgacgt tcccgacggt gcgatcatga tggtcgagga cggccagacg atcaaccgcg
     4561 gcagcatgct ctgcgagtgg gatccgcaca atatcccgat tctcgccgag gtcggcggta
     4621 aggtccgatt cgaggacatc gtcgaaggtg agacgatgaa gatcgagatc gatccgtcgg
     4681 gacacgtccg cagaacgatc atcgagcaca agggagatct ccacccgcag gtcatcatcg
     4741 tcgacgctga ggggaagaca ctcgattaca agtacattcc cgaacgggcc agtatcgagg
     4801 tcaacgacgg ccaaatgatc tcggccggca cgctcctggc caagaccccg cgtgaggtag
     4861 gaggaactca ggacatcacc ggcggcttgc cgcgggttac cgagcttttc gaggcccggc
     4921 gccccaaaga gcccgccgtg atcgccgaga tcgatggtcg cgtcgagcta ttggacgaaa
     4981 agcggcgcgg caagcgaacg atcatcgtcc gcaacgaaag cggtatcgag cgcgaacact
     5041 tggtgcccca cggcaaatac ctccgggtcc atggcggcga ccgcgttcga gccggcgacc
     5101 ccttggtcga gggcccgctg gtgccgcacg atatcttgcg aatctccggc gaagaggccg
     5161 tccagcgcta ccttctccgc gagatccaga acgtgtaccg gtcccagcgc gtcgaaatcg
     5221 acgacaagca cctggagatc atcatcgccc agatgctccg caaagtacgg gtcgacagcg
     5281 tcggcgatac gccgctgttg ccgggctcgg tgatcgata
//
LOCUS       DATAQL010001083         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2247690, whole genome shotgun sequence.
ACCESSION   DATAQL010001083 DATAQL010000000
VERSION     DATAQL010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247690"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..2170
                     /locus_tag="VHS97_18685"
     CDS             233..2170
                     /locus_tag="VHS97_18685"
                     /inference="COORDINATES: protein motif:HMM:NF006144.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding monooxygenase"
                     /protein_id="HEX3450288.1"
                     /translation="MQFYLNGYKPGDPFIEDPHPSVAERPGGLPEDADVLIIGCGPAG
                     LVLAAQMANFPDIRTVVIDRKDGPLEVGQADGVACRTVELFEAFGLADRLVNEGYWVN
                     EVAFWRPDPQDPTKITRTGRIQDVEESLSEMPHVIVNQARMLAYLLDHMERSASKLQP
                     FYGLHASDIQINTGGSSEYPVTVTLQHLKDFKETGEISTIRAKYVVGCDGSRSSTRAA
                     IGRELRGDPMNQSWGVLDALAVTDFPDIRLKCAIHSANQGNILIIPREGGYLVRFYIE
                     LDNVRDKEMLENRSVTPEKLATVANRILAPYTVEVKDVGWWAVYEIGQRLCDKFDDVP
                     VAEMATRLPRVFIAGDACHTHSAKAGQGMNVSMNDTWNLGWKLGAVLRGTAKPELLHT
                     YSEERQKIAKQLIDFDREFAKMFSAHPTECGSAGGEGVDPEEFRQYFITQGRFTAGVA
                     TKYGSSMITADAMFQHLAEGFPVGMRFHSAPVIRLADAKPIHLGHVARADGAWRVYIF
                     ADRKDPTSRHSYFRELCEFLESDASPIKRFTPAGAYPDSVIDVRAIFQQGHRDLAVDK
                     MPSVLLPKKGKFGLIDYKKMFCVDPKAGDIFELRGVNRETGCIVIVRPDQYVSHVLPL
                     HGHEALADFFAGILIDARQGR"
BASE COUNT          552 a          899 c          910 g          541 t
ORIGIN      
        1 cgcggccgcc aacataggcg tttcacgtca tcctgacctc ttgagtgaaa gccagagggg
       61 atgctgcgat gcacagtccg atagcgatca ccgttcgagt agcggatcat cggtcgtgct
      121 ggactattaa aactatcgcg gctccgcgct cgtcgagcac gtcatccgcc agcgcccgac
      181 gtatccgatc ttggtcgcgc ggccaccaca agcgttcaga ggggagtgaa cgatgcagtt
      241 ttatctgaac ggctacaagc ccggtgaccc gttcatcgag gacccgcatc cgtccgttgc
      301 agagcggccg ggaggtctcc ctgaggacgc cgacgtcctg atcatcggct gcggtccggc
      361 cggtctcgtg ctggctgcgc agatggcgaa cttccccgat atcaggacgg tcgtcatcga
      421 ccgcaaggat ggtccgctcg aggtcggcca ggccgacggc gtcgcctgcc gcaccgtaga
      481 gctgttcgag gcgttcggtc tggcggatcg tctggtgaac gagggctact gggtcaacga
      541 ggtcgccttc tggcggccgg acccgcaaga cccgaccaag atcacgcgta ccgggcgtat
      601 tcaggacgtc gaagagagct tgtcggagat gccccacgtg atcgtcaacc aggcgcgcat
      661 gctcgcctac cttctggatc acatggagcg gtcggccagc aagctgcagc cgttctacgg
      721 cctccacgcc agcgacatcc agatcaacac cggcggctcg tcggagtacc cggtgacggt
      781 caccttgcag caccttaagg acttcaagga gactggcgaa atctcgacga tccgggcgaa
      841 gtatgtcgtc ggctgcgacg gctcacgcag cagcacccgg gcggcgatcg gccgcgaact
      901 gcgcggggac ccgatgaacc agtcctgggg cgtcctagac gccttggccg tcaccgactt
      961 cccggacatc cggctcaagt gcgccatcca ctccgcgaac caaggcaaca tcctcatcat
     1021 tccgcgcgag ggcggatact tggtccggtt ctacatcgag ctggacaacg tgcgcgataa
     1081 ggagatgctc gaaaaccgga gtgtcacccc cgagaagttg gccacggtcg cgaatcggat
     1141 cctggccccg tacaccgttg aagtgaaaga cgtcgggtgg tgggcggtgt acgaaattgg
     1201 ccagcggctg tgcgacaagt tcgacgacgt gccggtagca gagatggcca cccgtcttcc
     1261 gcgggtgttc atcgcaggcg atgcctgcca cacgcacagc gccaaggccg gtcaaggcat
     1321 gaacgtgtcc atgaacgaca cctggaatct gggctggaag ctgggcgccg tcctgcgggg
     1381 gacggccaag ccggaactcc tgcacaccta ctcggaggaa cgtcagaaga tcgccaagca
     1441 actcatcgat ttcgatcgtg agttcgccaa gatgttcagc gcccatccga cggagtgcgg
     1501 cagcgcgggc ggagagggtg tcgatcccga ggagttccgg cagtacttca tcacgcaagg
     1561 ccgcttcacc gccggagttg cgaccaaata cggctcgtca atgatcaccg ccgacgcgat
     1621 gttccagcac ttggcggagg gcttcccggt cgggatgcgc ttccactctg ccccggtcat
     1681 ccggctggcc gacgccaaac cgatccacct cggccacgtc gcccgggcgg acggcgcctg
     1741 gcgcgtctac atcttcgctg accgaaagga cccgacgagc agacactcct acttccggga
     1801 gctgtgcgag ttcttggaat cggacgcgtc tccgatcaag cgcttcacgc cggcgggtgc
     1861 gtaccccgac tccgtgatcg atgtccgtgc catcttccag caggggcatc gtgacttggc
     1921 tgtggacaag atgccgtcgg tacttctccc gaaaaagggc aagttcggcc tgatcgacta
     1981 caagaagatg ttctgcgtcg acccgaaggc cggggacatc ttcgagctgc gaggggtgaa
     2041 tcgcgagacg ggctgcatag tcatcgtccg cccggaccag tacgtctcgc acgtgctccc
     2101 actccacggc cacgaggcat tggcggactt cttcgccggg atcctgatcg acgctagaca
     2161 ggggcgctag atcggcgctt tggtgcatga atagggaaaa gggcgaggac cttccttcga
     2221 atttcacgca ttaggcgatg acgcgcggga gtgttcagag actggggggg agggacggaa
     2281 gcgatatgac cggcttgcga aaggtcttca gacgctccca atttcgcgtg atgaagtcgg
     2341 ccttgtatgc ggccatgtaa tccatggacc tgatcgccga atgtcggcgc cggccgcgaa
     2401 tgccttgtcc gatccgggca gcacgacgca ggagatgttc tcgtccgcct cggactcgtc
     2461 cagagccttg ctgagcgaat ccatgacttc gtcgttccgc gcgttgtaac ggcgggatgg
     2521 ttgatgcgga tcaagccgac cttttctcgc ctttcggcaa ggattaccgg tgacatgtgg
     2581 gcctccaagc gatcaagggt gactgggcgt tccgccgcgc ggcgcgcatc gcgtcgattt
     2641 acgaggcgtc gccgaacgcc tttgccattt cccgctggcg gcgtcaggtc aaccgctgcc
     2701 actataggcc gcatggcccc gaatggacgc ttaggcgtgc gggttcccta taactgtcgt
     2761 tccctttgca gccatacgta cctggaactg aatgtctgat ttccccgtcg cgtccccgag
     2821 agcggaccgt cggcttttca tctgtggacg ccccctcccg gtcaaggaaa aacgtcagga
     2881 ttatctgagc acgtagtcgg at
//
LOCUS       DATAQL010001084         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2247879, whole genome shotgun sequence.
ACCESSION   DATAQL010001084 DATAQL010000000
VERSION     DATAQL010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247879"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VHS97_18690"
     CDS             <1..449
                     /locus_tag="VHS97_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450289.1"
                     /translation="LDLLLPLLLETRELERLADVAGRSEDSALERVSHLTTEPAARKL
                     EKTHPGIAARLWRAQGMRVLHAKKSKYYPEALENFRRARRCYKKAGLSSQWLRVVDQL
                     RTGHHRKVGFMAGFEELVAGKPPREKPSFLDEAKARWGLPQAANRK"
     gene            484..1329
                     /locus_tag="VHS97_18695"
     CDS             484..1329
                     /locus_tag="VHS97_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450290.1"
                     /translation="MDLETLKDTPPWDWPEGTAGELLCIVRRERAAPERVRVLAIQLA
                     GDFTVINEELVDALLAVLHDSDESQKSRARAAISLGPVLDYMHTEDIENEYFDDEGEL
                     PISAETFERIQTSLRTLYEDAKIPKEVRRRILEASVRAPEDWHVDAIRAAYARGDEAW
                     KLTAVFCMRFVRGFDEQIVKELDSKNADIHFEAVVAAGNWGVEGAWADVTALIHSAKT
                     PKPLLLAAIEAVPHIRPKEAMDALSELRESDDEEIVDAIDEALAMAGEPSEEDEEDED
                     DLFLA"
     gene            1362..1670
                     /locus_tag="VHS97_18700"
     CDS             1362..1670
                     /locus_tag="VHS97_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450291.1"
                     /translation="MKKRNRKREEWTGARERHHLSDIQVQMARELGMNPAKLGKLDNS
                     KQEPWKMPPGPYIEHLYRKRFEKAYPDNVTSIKERSGLSEEKREARHRSRQQLLRGTP
                     "
     gene            1667..>3019
                     /locus_tag="VHS97_18705"
     CDS             1667..>3019
                     /locus_tag="VHS97_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450292.1"
                     /translation="MKSYDAQDRIPTLAVALNDFMNSDDLKKLGAVTGQKKLPTRKAD
                     LAEVILRHLEGDRLRIVWQSLDEIQRAAVAEVAHSSGTKFPADQFRAKYGKDPNWGST
                     EEHEYKRVPSALRFFFYGSRIMPDDLKKRLKEFVPPAAKAKVESLDELPAVYDRPFER
                     WNEKKKKSEHHTEAVTLVVYESGRGAQRELFSVLRLVDAGKISVSEATHRASAVSMDA
                     ITAVFDAGDYYAFVPPKSKWHDDNAGPIRSFAWPLLVQAGGLAKRSGSKLQLTPAGRK
                     ALTEPAAETLKVLWESWTYNTLVDEFSRIDAVKGQTGRAKRGLTDVSCRREAISIELA
                     ECPVGRWISTAEFIRFLRASGCDFAVTENPWGLYLSDPQHGSFGYEGSARFLDERYVL
                     CLLFEYAATVGLIDVAFIPPAGARRDFHALWGVDDLSFLSRYDGLMYFRVTPLGAYSL
                     G"
BASE COUNT          669 a          865 c          921 g          564 t
ORIGIN      
        1 acttggattt gctactgccg ctacttcttg agacaagaga actcgaacgg ctcgccgacg
       61 tcgccggccg gagcgaggat agcgccctcg agcgcgtgag ccaccttacg accgagcccg
      121 cagcgaggaa actcgagaag acccatcccg gcattgcagc acggctttgg cgtgcccagg
      181 gcatgcgtgt ccttcatgcg aaaaagagca agtactatcc cgaggccctc gagaacttca
      241 ggcgcgcgcg acgttgttat aagaaggcag ggctttcgag tcaatggctg cgcgtcgtcg
      301 accaactgcg caccggtcac catcgcaagg tcggttttat ggcgggtttc gaagaacttg
      361 ttgccggcaa gcctccccgc gagaagccgt cgtttctcga cgaggcgaag gcacgctggg
      421 gcttgccaca ggctgcgaac aggaaataaa gccactctat ggtggcggaa gggagggacc
      481 gagatggatc tcgagacttt gaaggacact ccaccctggg attggccgga aggcacggcg
      541 ggagaactgc tttgcattgt tcgaagggag cgtgccgcgc cagagcgggt acgcgtttta
      601 gccatccagt tggcgggtga tttcacggtg attaatgagg agttggttga tgcgctgctc
      661 gcggtgctac acgacagcga cgaatcccag aaatcgcgtg ctcgcgcggc gatttcgctc
      721 gggccggtac tggactatat gcacaccgaa gacattgaaa atgagtattt tgacgacgaa
      781 ggcgaactcc cgatctccgc cgagactttc gagcggattc agacgtcact tcgcacgctc
      841 tacgaggatg ccaagattcc caaggaggtc aggcggcgaa tccttgaggc atccgtgcgc
      901 gcacccgagg actggcatgt ggatgccatc cgtgccgcct acgcacgcgg cgatgaagcg
      961 tggaagctta ccgcggtctt ttgcatgcgc tttgtccgcg ggttcgacga gcagatcgtc
     1021 aaagagctcg acagcaagaa cgcggacatt cacttcgagg ctgtcgttgc cgcgggaaac
     1081 tggggcgtcg aaggtgcctg ggcggacgtt acagccctca tccattcggc caaaacccca
     1141 aagcccctgc tgctggccgc gattgaagca gtaccccaca ttcgtccaaa ggaagccatg
     1201 gacgccttga gtgaactgag ggagtccgac gatgaggaga tcgtcgatgc gattgatgaa
     1261 gcgttggcaa tggcgggtga accctccgaa gaggacgagg aggacgaaga cgacctcttc
     1321 ctcgcataaa atgtaacgca ctcgagaaga atgccgtggc catgaagaaa cgaaaccgaa
     1381 agagggagga gtggaccggt gctcgagagc gccatcacct ctctgacatc caggtgcaga
     1441 tggctcgcga gttgggtatg aaccccgcta agctcggcaa gctcgacaat tccaagcagg
     1501 aaccctggaa gatgccgccc ggaccgtaca tcgagcacct ctaccgcaaa cggttcgaaa
     1561 aggcctatcc cgataacgtt acgtccatta aggagcgctc gggcctctcc gaggagaaga
     1621 gggaagcgcg gcaccggtcg aggcaacaac tcttgagggg gactccatga aatcctacga
     1681 cgcgcaagac agaatcccga cgctagccgt ggcgcttaac gacttcatga attcggatga
     1741 cctcaagaag ctcggcgcgg tcaccgggca gaagaagctg ccaacccgaa aggccgatct
     1801 cgccgaagtc atcctccgtc acctcgaagg tgatcgtctc cgcattgtct ggcaaagcct
     1861 tgatgagatc cagcgagccg ccgtggccga agtcgctcac tcgagcggga caaaatttcc
     1921 ggccgatcag tttcgcgcca agtacggaaa agatcccaac tggggctcaa cagaagaaca
     1981 tgagtacaaa cgagttcctt cggcactgcg atttttcttc tatggcagcc gaatcatgcc
     2041 ggatgacctg aagaagcgtc tcaaggagtt tgtgccgcct gccgccaaag ccaaggtgga
     2101 atccctcgac gagttgccag ccgtatacga ccggcctttc gagcggtgga acgaaaagaa
     2161 gaaaaagtcg gagcaccaca ccgaggcagt aacgctcgtg gtgtacgaat ccgggcgggg
     2221 ggcccagcgg gagctctttt ccgtgcttcg tctggtggat gccggcaaaa ttagcgtgag
     2281 cgaggcgacg cacagagcct ccgccgtgag catggacgcg atcaccgccg ttttcgatgc
     2341 cggcgactac tacgcattcg tcccgcccaa gagcaagtgg catgacgaca acgccggacc
     2401 aatccggtcg ttcgcatggc cgctgcttgt tcaggcaggc ggccttgcca agcggtcggg
     2461 gtcgaagctg caactcacgc cagccgggcg gaaggccctt acagagccag cggcagaaac
     2521 cctgaaagtc ctgtgggaga gctggaccta caacacgctc gtcgacgaat tttctcgaat
     2581 cgatgccgtc aagggtcaga ccggcagggc caaacgcggg ctgacggacg tgtcgtgtcg
     2641 ccgtgaggca atttcgatcg aacttgcgga gtgcccggtc gggcgatgga tctccaccgc
     2701 ggaattcatc aggttcctgc gggcttcagg ttgcgacttt gcggtgacgg agaacccctg
     2761 gggtctctac ctctcggatc cgcaacacgg ttcttttggc tacgaaggta gtgccaggtt
     2821 tcttgacgag cgctacgttc tctgtcttct tttcgaatat gccgccaccg taggactcat
     2881 cgacgtcgct ttcattcccc cagcgggagc gcggcgcgac ttccacgcac tttggggagt
     2941 cgacgatctt tccttcctca gccggtatga cggcctcatg tattttcgcg tcacgccgct
     3001 gggggcctac tcccttggt
//
LOCUS       DATAQL010001085         5632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3623482, whole genome shotgun sequence.
ACCESSION   DATAQL010001085 DATAQL010000000
VERSION     DATAQL010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623482"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="VHS97_18710"
     CDS             complement(<1..1635)
                     /locus_tag="VHS97_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450293.1"
                     /translation="MTRFVHHAVSVVSVLLALAGTGGSRWQICSAQEELVERTGPPAA
                     DNLEVDQNNDGLPDGWYNARDAKWITEGGAPDAGPHFVRFECKDPGRPARLSRAFGVD
                     GRKTEAVILGLWIRQSNVQFGEREGDEPSLMIDFLGDELRHLSRGSLGPWTRLARDRW
                     TRVAKRIPVPLGTKDAILSVGLMGATGILDIDGLTVDLVARGGEETTNLIVNGGFELG
                     DPSPASWVIDNDAHRAFPGNRSPAALELTRARSHAMTGVAIPVAQFEWLDVTVAVQCS
                     RLRGAGGAAAAFFFVNDLGKPLPGPDGTNYFLNWAGTSPWHIEQARVPVPVGAARAVL
                     QFEKLDPIGWIRIDDVEVAASPNPAAGAWTPYHTADDTVEWLPAPASKAIEANSALDV
                     SFLLPRPAGALGFVTVKDGRLTWEKGGGRARFFGVALLPPAAFLPSEQADLLADRLAR
                     SGVNLVRFGDLDSAYGPNRSLFDDTRDDTKAFDSEALARLDHLIAALKSRGIYVALEL
                     QGKRRFRTGDGVTTPGMLPPGGGPAALFDPTLSKLAI"
     gene            complement(1690..2529)
                     /locus_tag="VHS97_18715"
     CDS             complement(1690..2529)
                     /locus_tag="VHS97_18715"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX3450294.1"
                     /translation="MPARYLVQVGVSLAVASTLVLAPRIGAQQRNADGVPAKDLSKKD
                     DPTEPARKRMVERHLVERGIKNPRVLDAFRTVPRHRFLPPGTQRQAYDDESIPIGEGQ
                     TITPPYDVAFMTEVLNPKPTDRVYEVGTGSGYQSGILSRLVKDVYSVEIHEPLSKRAT
                     KVHQDLGYTNIHTRVGDGYVGWPEAAPFDAIIVTCAPTKVPQPLFDQLKEGGRLVIPI
                     GDRFNQYVHLIIKKDGKRVDSVLKPTLFVPMTGRDQEEAAAARRASERSQKSTTKDAP
                     QNK"
     gene            2828..3289
                     /locus_tag="VHS97_18720"
     CDS             2828..3289
                     /locus_tag="VHS97_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450295.1"
                     /translation="MQAPARSLFLLLALASAAWTYPTSPSESVTLRGKVVTLVQALES
                     RGLGLKPDSEPIAKQIVLLAEDGSIVPLLSDDGSRALFLDERLRNCRSEIKGRRFVGV
                     PYIQVLSFKVEMGGKLQTPEYYCDICAISVPYPRICDCCQGPTTLRARPER"
     gene            3424..4650
                     /locus_tag="VHS97_18725"
     CDS             3424..4650
                     /locus_tag="VHS97_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ceramide glucosyltransferase"
                     /protein_id="HEX3450296.1"
                     /translation="MSAAESANLLLGLVAVVAVVSTLGGIGCVFWVTRSRGRRSDYSP
                     PVTIFKPLKGLDEELEQNLRSFFQLDYPCYQLLFCVADADDPAIHVVRQLQREFPDHD
                     SQLIVGCPAFGLNPKVESLAAMEPQRKHDVILISDSNVRARPSYLRETVCYLAEPGVG
                     LVSNLFAGTGEVHTSAVLENLQLNGFIAGGVAAASVCGATCVVGKSMLMPVTALEAIG
                     GLAAVRNVLAEDQVIGVRVRQAGYSIRLSHHVVQNINRDRSFKWFLNRHSRWYKIRRQ
                     MALPAFVAEPAASLATVGLVWALSGDSGIAWGGLLLLVGLGMERDAVQTRRLRGSYPK
                     LRHLMYSPVKDILLLPVWLDAIVNRRVHWRGHRFLIGRMTRLRVARVPRQVRKRVQRV
                     RRFRSQNPQERDRQAQ"
     gene            4955..>5632
                     /locus_tag="VHS97_18730"
     CDS             4955..>5632
                     /locus_tag="VHS97_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3450297.1"
                     /translation="MFTSCDAAQGDALDRIHSAGRLRYGSDMEGGGPYAYPDPSSPRD
                     VTGFEVELMASLGKGLGANPQFSQGQWDKLLQLLDSGRIDLVCNGYEWTETRAHDYLS
                     TRPYYVYQLQLMARRGGTIRSWADLKQRPKPGGGRWTVGVLVGSAGDTFAAEQGGSLV
                     EVIRFDGATDAMLAVQNGQYDATLQDVPAARFYQERFPGLELVGPAESHGYYVLYVRK
                     EERGLRDG"
BASE COUNT         1027 a         1673 c         1775 g         1157 t
ORIGIN      
        1 gatcgcgagt ttgctcaacg tcgggtcgaa caacgccgcg ggaccgccgc cggggggtag
       61 catgccgggt gtagtaactc catctccggt ccggaatctt cttttgccct gaagctcgag
      121 cgccacgtag atgccgcggg acttgagcgc ggcgatgaga tggtcaagac gggcgagtgc
      181 ttcggaatcg aacgccttgg tatcgtcacg cgtgtcgtca aaaagactgc ggtttggtcc
      241 gtaagccgag tcgaggtcgc caaaacgtac caggttgacc ccggaccgag ccaaccggtc
      301 ggcaaggagg tcggcttgct cggacggcag aaatgccgcg ggcggaagca acgcaactcc
      361 gaaaaatcgc gctcgtccgc cgcctttttc ccaggtgagc cggccgtcct tgacggttac
      421 aaacccgagc gcgccggctg gtcgaggcaa tagaaacgag acgtcgagtg cgctattggc
      481 ttcgatcgct ttcgaagcag gggccggcag ccactcgacg gtatcatcgg cggtgtgata
      541 aggggtccag gcgcccgccg cggggttggg cgaggccgcg acctcgacgt cgtcgattcg
      601 gatccagccg attgggtcta gtttttcaaa ctggaggacc gcccgcgccg cgcccaccgg
      661 cacggggacg cgggcctgtt cgatgtgcca ggggctggtg cccgcccagt tgaggaagta
      721 gttggtgccg tcaggaccgg gcaatggttt acccagatcg ttgacgaaga aaaacgcggc
      781 ggctgcgcct ccagctccgc ggagacgcga acattgaacc gcgacggtca cgtcgagcca
      841 ctcgaattgt gcaaccggaa tggccacgcc ggtcatcgcg tgtgacctcg cccgtgtcag
      901 ttccagggcc gccggcgagc gattaccggg aaatgctcgg tgggcgtcgt tgtcgatgac
      961 ccacgatgcg gggctcgggt cgcccagctc gaagccacca ttcacgatca agttggtggt
     1021 ctcctcgccc cccctcgcaa cgagatccac ggtaagcccg tcgatgtcga gtattccggt
     1081 cgcgcccata agtccgaccg aaagtatggc atccttggtt cccagcggca ccgggatgcg
     1141 tttggcgacc cgcgtccacc ggtcgcgcgc caaacgggtc caggggccca agctgccccg
     1201 actcaaatgg cgcagctcgt cccccagaaa gtcgatcatc aggctgggct cgtctccttc
     1261 gcgctcgccg aactgcacat tgctttgcct gatccacagt ccgaggatca cggcttcggt
     1321 cttgcgccca tcgactccga aagcgcggct gagccgcgcc ggcctgcctg gatccttgca
     1381 ctcaaaccgg acaaaatgcg ggcctgcgtc gggcgcaccc ccttcggtta tccatttggc
     1441 gtcgcgcgcg ttataccagc cgtcgggcag accgtcgttg ttttggtcca cctccagatt
     1501 atcggccgcg ggtggcccgg tacgctcgac gagctcttcc tgagccgagc aaatttgcca
     1561 acgacttccg cccgtgccgg caagcgcgag caaaaccgat acgaccgaca cagcgtggtg
     1621 aacaaacctc gtcacgtcga cacctcggca ttcgcccctc gcgagccggg acgcctctcg
     1681 tcccgatcgt catttgttct ggggagcatc ttttgtggta gatttttggg aacgctcgga
     1741 agctcgcctc gcggctgccg cctcctcctg atcgcggccc gtcattggta cgaacagggt
     1801 cggcttgagc acactatcga cccgtttgcc gtcttttttg atgatgaggt ggacgtactg
     1861 gttgaaacga tcgccgatcg gaatcaccaa gcgtcctcct tccttgagct gatcgaacag
     1921 gggttgaggg acttttgtgg gagcgcacgt gacgatgatc gcgtcgaacg gcgcggcttc
     1981 cggccagccg acgtagccgt cgcccactcg agtatgaatg ttggtgtagc caagatcttg
     2041 atgcaccttg gtggcacgtt tggagagagg ctcgtggatc tcgacggaat aaacgtcctt
     2101 gactagtcgc gagagaatcc ctgattgata gcccgagccc gtgcctacct cgtaaactcg
     2161 atcggtcggc ttggggttaa gcacttcggt catgaatgcg acgtcgtagg ggggggtgat
     2221 tgtctggcct tcgccgatcg gtatcgattc gtcgtcatac gcctgacgct gggttccggg
     2281 cggtaggaag cggtgtcggg gcacggtgcg aaaggcgtcc aggacgcgcg ggtttttgat
     2341 gccacgctcg accaagtgcc gctcgaccat tcgttttcgc gcaggctcgg taggatcgtc
     2401 tttcttactc aagtctttgg ccggcacacc gtcggcgttc cgctgctggg ctccgatgcg
     2461 cggcgccagg acaagggtcg aagcgacggc caagctcact ccaacctgga ccaggtatcg
     2521 tgcgggcatg gatcggtcct tcgtgaattt caggaaaatg gccttgcgct cacgtaccga
     2581 gagaggattg cttgcctctt atcatactcc gaaatgagcc tacaaggggg catgtcgatc
     2641 aaagtgaagg gctgaatgag acatcgggac gaacgagcag gactgagtgc cgccaatttg
     2701 gagccttttc gcggtccgcc tgaaatacgc ttggtccccg aaggtgacgc cgttataatt
     2761 catcaatccg tagccagggg tgcaagctca agtcgttgtt tctcaacggc ctggagaaaa
     2821 atcgcagatg caggctccgg cgcggtcatt gttcttactt ctggctcttg cttccgcggc
     2881 ttggacttac ccgacctcgc cgtctgagtc ggtcacgctg cgggggaagg tagtaacttt
     2941 ggtccaagcc ttggagtcgc gggggctcgg tctcaagcca gactcggagc cgatcgccaa
     3001 gcaaatcgtg ctcctggcag aggatggatc cattgttccg cttctttccg atgacgggag
     3061 ccgagcacta ttcctcgacg aacggctgcg taattgtcga agcgagataa agggccgccg
     3121 atttgtgggt gtcccctata tccaggtgtt atcgttcaaa gtggagatgg ggggcaagct
     3181 ccagactcca gaatattatt gcgacatctg cgcgatcagc gtaccctatc cgcggatttg
     3241 tgattgctgt caaggtccca cgacgctgcg tgcgcgaccc gaacgatgaa ttgaatcgga
     3301 gccgccgcga tggcagcgct tgaacaaagc acgcgggcca gaccggtggc ggatcgccca
     3361 agaacctgat tcgatctgct gcgagtgacg gcggcgaacc gcgccgcaag gggatctttg
     3421 tacatgtcag ccgccgaaag cgccaatctg ttactcggtt tggtcgcggt cgttgccgtg
     3481 gtctcgacgc tgggcgggat cggttgcgtc ttctgggtca ctcggtcccg cggtcgccgg
     3541 tccgactata gcccgcccgt gacaatcttc aagccgctca aggggctcga cgaagagctc
     3601 gagcaaaacc tgcggagttt cttccagctc gattacccgt gctatcagct tctcttttgc
     3661 gtcgccgacg cggacgatcc cgccatccac gtcgtccgtc agctccagcg cgagtttccc
     3721 gaccacgatt cccagctcat cgtcggctgt ccggcgtttg ggctcaatcc caaggtcgaa
     3781 agcttggcgg ccatggagcc ccagcgcaag cacgacgtga ttttgatcag tgattccaac
     3841 gtacgtgccc gtccgtccta tttgcgagaa acagtttgct atctcgccga gccaggggtg
     3901 gggctcgtga gcaatctgtt cgccggcacg ggcgaggtac acacaagtgc ggtcctggaa
     3961 aacctgcaat tgaacggctt catcgctggc ggcgttgcgg cggcgtcggt ctgcggcgcg
     4021 acgtgcgtgg tcggcaagtc gatgctgatg cccgtcaccg ccctcgaagc catcggagga
     4081 ttggccgcgg ttcgcaacgt cttggccgag gatcaggtga tcggcgttcg tgtccgccag
     4141 gcgggctatt cgatacgctt gagccaccac gtcgtccaga acatcaatcg cgaccgcagt
     4201 ttcaagtggt tcctcaatcg tcattcgcgc tggtacaaga ttcgccggca gatggcacta
     4261 cccgccttcg ttgccgagcc cgcggctagc ctggcgactg tgggcttggt ctgggcgtta
     4321 tcgggcgatt caggaatcgc gtggggaggg ctgctattac tggtgggcct gggaatggaa
     4381 cgggacgccg tgcagacccg ccggttgcgc gggtcgtatc ccaagctgcg gcatctcatg
     4441 tacagtccgg tgaaagatat cctgttgtta ccggtctggc tggacgcgat cgtgaaccga
     4501 cgggtgcact ggcgtggcca ccgattcttg atcggacgga tgacccggct gcgcgtcgca
     4561 cgcgtgccac gtcaggttcg aaaacgggtg cagcgggttc gccggtttcg ttcgcagaat
     4621 ccccaagagc gagatcgcca ggcgcagtga tcgatcagcc ccgactgtgt gtcgatttcg
     4681 cgcgccagcg ccttgaacat tgtctcccga aatcgaatga taaagtcagt gtgcacttct
     4741 ggcccatggg cttccggacg gacggaggtt cttgatggaa gaaaactccg gcgcatcgga
     4801 atccgatgct gtttgttctg tcccttcttt caagactcgc caagcgacgt ccgcgcgagc
     4861 cggcgaagcc atggggccat tcttcgcctg ccgtcgtaac atccactttc cccgtacgtg
     4921 cgcggctgta tcagccatcg cggttttttt tctcgtgttt acatcctgcg atgccgcgca
     4981 gggtgacgca ctcgatcgga ttcacagcgc gggacgcctg cgatacggta gcgatatgga
     5041 aggaggggga ccttacgcct accccgaccc cagttcaccg cgcgacgtca ccggatttga
     5101 agtggaattg atggcctcgc tggggaaggg cctgggtgcg aatccgcagt tttcgcaggg
     5161 tcagtgggac aagctgctgc aactgctcga ctctggtcgc atcgaccttg tgtgcaatgg
     5221 gtacgaatgg accgagacgc gcgcacacga ctacctgagc acacgtccct actacgttta
     5281 ccaactgcag ctgatggcac ggcgcggcgg aacgatccgt tcgtgggccg acctcaaaca
     5341 gcggcccaaa ccggggggcg ggcgctggac agtgggcgtt ctggtcgggt cggcgggtga
     5401 cacattcgcc gccgagcaag gaggttcgct ggtcgaggtg atccggttcg acggggccac
     5461 ggacgccatg ttggcagttc aaaacggcca gtacgacgcc acgttacagg acgtgccggc
     5521 ggctcggttt taccaggagc gcttccccgg cctcgaactt gtcggtccgg ccgagtccca
     5581 cggctattac gttttgtatg tacggaaaga agagcgaggc ctccgtgatg gc
//
LOCUS       DATAQL010001086         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3928731, whole genome shotgun sequence.
ACCESSION   DATAQL010001086 DATAQL010000000
VERSION     DATAQL010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928731"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2174)
                     /gene="polA"
                     /locus_tag="VHS97_18735"
     CDS             complement(<1..2174)
                     /gene="polA"
                     /locus_tag="VHS97_18735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX3450298.1"
                     /translation="MATRPSFYVLDAYSLIFQVFHAIPEMTGPDGQPTQAVFGIFRDL
                     LNIVRDRKPDYLAAAFDGPGRVFRSDIYEDYKANRKEMPVDLVPQIPVIRRVFEGFRV
                     PVLTVPEMEADDIIATLARRAEERGLDVFIVTADKDARQLISEQVRLLNLRKNKIIDA
                     AELLKEWGVRPDQVVDFLALTGDAVDNVPGVPGIGEGFATTFLKQFGTLEKLLANINQ
                     VKGPKKQQSLREHADTARRARQLVTLRDDLALELDWDALKLQSPDMESLKLLCTECGF
                     HRFRDELGAQQRELPLMKSEPAWEAVYRAVDTPELFATFLDELKRQSRFSVDTETTAI
                     DPLRADLVGLSFSWKAGEAYYLPVRGPAGSNLLDENETLAALRPILADPDIEKIGQNI
                     KYDMLALGRAGAPLEGPVTDTMILSYLLESGERNHNLDQLSQRLLDHTMVPITDLIGK
                     GKSQGRMDQVPVDRVAYYAGEDADATWRIEAILSPKVRDQGLWQLYDEVERPLIAVLA
                     RMEAAGITVDVERLRQLAREFGDRITTIETEAFSLAGRTFNINSGPQLRQVLFDELKL
                     PSLQKTPGGEQSTAQDVLEQLALKHPFPKLLLQHRQLSKLKSTYLDALPGLVHPEDNR
                     IHASFNQSVAATGRLSSSDPNLQNIPVRTEDGRQIRQAFIAGRPGWLLLTADYSQIEL
                     RVLAHYSADPALFRAFANDLDIHKAVAARIFGVAETAVDDSMR"
     gene            2552..>3821
                     /locus_tag="VHS97_18740"
     CDS             2552..>3821
                     /locus_tag="VHS97_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450299.1"
                     /translation="MAAANESQGLKIAVATFIALTVILTVTSYFLYTAYSQADARLTS
                     ANDQLAQKSKESSLALNQYDEVRGKVGTKNQEYEQVKEEINAHFKKMDTRLENVTNAV
                     NAAVQKAQADGAQGTELEEAKQNIQKIIASLRSEPNKSYISSIDRLQELFENLALLST
                     ELSLNYVNLRKSLESATSVAKQQVDVEAKAAEKSQADVLGEQKKHEDERGILVTKVTE
                     LQTANDKQATQIANLENKNKQQEEDFLRKQETYTTIIREQRDNIERKELILDRPDGYV
                     TYVDYERGEVLVSLTKRQGARPQMKMTIFDAKSPGIPTEKPKGNIELTQVGEQFSAAR
                     IVKTISSIDPIRVGDIVYSAAWSPNMPMRFALVGKMDVNRDGKDDRDELKRMIQEAGG
                     TIDFDLPPADLGKETGTLSPRIDWYVIDNRM"
BASE COUNT          837 a         1131 c         1126 g          727 t
ORIGIN      
        1 cgcattgaat cgtcaacagc cgtctcggca acgccgaaga tccgcgctgc cacagccttg
       61 tgaatgtcga gatcgttggc aaacgcccgg aagagtgccg gatcggccga ataatgggcg
      121 agcacgcgca gctcgatctg cgagtagtct gctgtcaaaa ggagccagcc cggccggcca
      181 gcaatgaaag cctggcggat ctggcgaccg tcctcggtgc gcacggggat attctgcaag
      241 ttggggtcac tcgagctcag ccgccccgtt gcggcgacac tctggttgaa cgacgcgtga
      301 atgcggttat cctcgggatg cacaagtcca gggagtgcgt ccagataggt gctcttgagt
      361 ttggagagct ggcgatgttg caagagtagc ttggggaatg ggtgcttgag cgccagttgt
      421 tccaggacgt cctgggcggt gctctgctca ccacccggcg tcttctgcaa cgaaggcagc
      481 ttgagctcgt caaaaagcac ctggcggagc tgcggccctg agttgatgtt gaaagtacgg
      541 cccgcgagac tgaacgcctc ggtctcaatc gtcgtgattc ggtccccgaa ttcgcgcgca
      601 agctgccgca accgctcgac gtccaccgtt attccggccg cctccatccg ggccagcacg
      661 gcgatcagcg gccgctctac ctcgtcatag agctgccaaa gcccttgatc gcgaaccttg
      721 ggcgaaagga tcgcctcgat tcgccaggtc gcgtcggcat cctcaccggc gtaataggcg
      781 acccggtcga ccggcacctg gtccatccga ccctggctct tccctttgcc aatcaggtcg
      841 gtaatcggga ccatcgtgtg atcgagcagc cgctgggaca actggtcgag gttgtggttg
      901 cgctcgccgc tttcgagcag gtaactgagg atcatcgtgt cagtgactgg cccttccaga
      961 ggcgcgccgg cccgccccag cgccagcata tcgtatttga tattctggcc gatcttttca
     1021 atatcggggt ccgcgaggat tggtcgcagg gcggccagag tctcgttttc gtcgagcaaa
     1081 ttggatccgg ccggcccgcg gaccggcaaa taataagctt ctccggcctt ccagctaaac
     1141 gacagcccga ccagatccgc tcggagcggg tcgatcgcgg ttgtctcggt atcgacgcta
     1201 aacctggact ggcgcttgag ctcatcgagg aaagtggcga aaagctcggg cgtatcgacg
     1261 gcgcggtaga cggcctccca ggccggctca gacttcatta agggcaattc ccgctgttgg
     1321 gcgcccagct cgtctcgaaa ccggtgaaac ccacattcgg tgcagagcag cttcaaagac
     1381 tccatatcgg gcgactgtaa tttgagcgcg tcccaatcga gctcgagcgc caggtcgtcg
     1441 cgcagagtta cgagctgccg agcccgccgg gcggtatcgg cgtgctcgcg caggctttgc
     1501 tgctttttgg gccccttcac ctggttgatg ttggcgagga gcttttcgag agtgccaaac
     1561 tgtttcaaaa acgtggtcgc aaacccctcg ccgatgcccg gaacgccggg cacgttgtcg
     1621 accgcgtcgc cggtcaaggc caggaaatcg acgacctggt caggacggac gccccattct
     1681 tttaggagct cggcggcgtc gatgatcttg tttttgcgca ggttcaagag acgaacctgc
     1741 tcgctgatca actggcgagc gtccttgtct gcagtgacaa taaagacgtc taaaccccgt
     1801 tcctccgctc ggcgcgcgag cgtcgcgatg atgtcgtcgg cttccatctc gggcaccgtg
     1861 agcacgggca cccgaaaacc ctcgaacacc cggcggatca ccgggatctg cggcaccagg
     1921 tcgacgggca tttccttgcg gttggccttg tagtcttcat agatgtccga gcgaaaaacg
     1981 cgaccaggcc cgtcgaaggc cgccgccagg taatcgggct tgcggtccct gacgatgttg
     2041 agcaaatcgc ggaagatccc gaagacggcc tgggtcggct gcccgtcggg gcctgtcatt
     2101 tccgggatcg cgtggaatac ttgaaagatc agggagtagg cgtccaggac atagaaactg
     2161 gggcgtgtcg ccatcgctct gtcgtctccc ttgccgtgcc gtagaattct gcggaactat
     2221 tacggcccgc gcgcatcaaa agtgatgggg cgggcatgcg ctctcaatta ctgcgttgct
     2281 gtccgcactt tcgataccgc gactgactga accaaagcca atcaccgccg atcaattgcc
     2341 gccggtgtcg cgggatttga ccggtactct gacggttctt acaatgggaa ggatcacagg
     2401 gactttcccg atcaaacgct cctcgacgaa tccgtcgggg gagaaatttg tcagcttgcc
     2461 cgtcgtttcc tctcgcatgt gacatagtat cagcaaaccg gcggattgca tgagcaggtc
     2521 gctaccgctc cgctcggggg agtaaccatt catggccgca gccaacgagt cgcaaggtct
     2581 caagatcgcc gtcgccacgt tcatcgcgct gacggtcatt ttgaccgtca cgtcgtactt
     2641 cctgtatacg gcctactcgc aggccgacgc caggctgaca tcggccaacg atcaactggc
     2701 gcaaaagagc aaggaatcga gcctggccct caatcagtac gatgaggtcc gaggcaaggt
     2761 cggcaccaaa aaccaggagt acgagcaggt caaggaagag atcaacgctc acttcaagaa
     2821 gatggacaca cgactcgaga acgtcaccaa cgcggtcaac gccgccgttc agaaagccca
     2881 ggccgacgga gcccagggaa ccgagctcga agaggccaaa caaaacattc agaaaatcat
     2941 tgcatccctt cggagtgagc ctaataagag ctacatctcg tctatcgatc gcctgcaaga
     3001 gcttttcgag aacctggcgc ttttgagcac ggagctctcg cttaactacg tcaatctccg
     3061 caagagcctg gaatcggcca ccagcgtcgc caagcagcaa gtagacgtcg aagccaaggc
     3121 ggctgaaaag agccaggccg acgtcctggg cgaacaaaag aagcacgaag acgagcgggg
     3181 tatcctggtc accaaggtca ccgaacttca aacagccaac gacaagcaag ccacccagat
     3241 cgccaatctg gaaaacaaaa ataagcaaca ggaagaagat tttctgcgca aacaggaaac
     3301 atataccacc atcatccgcg aacagcgcga caatattgag cggaaagagc tcattctcga
     3361 tcgaccagac ggctatgtga cgtatgtcga ttacgagcgg ggggaggtat tggttagcct
     3421 cacgaagcgc cagggcgctc gcccgcaaat gaagatgacg atctttgatg caaagtcgcc
     3481 tggcattccc accgagaaac ccaagggaaa catcgagttg acgcaggtcg gcgagcagtt
     3541 tagcgccgcc cggatcgtca agaccatcag ctcgatcgac cccattcgtg tcggcgatat
     3601 cgtttactcg gcggcctggt cgcccaacat gccgatgcgg tttgccttgg tgggcaagat
     3661 ggacgtgaat cgtgacggca aggacgaccg cgatgagctc aagcggatga ttcaagaggc
     3721 cggcggcacg atcgacttcg accttccccc cgcggatctc ggtaaggaaa ccggcacgct
     3781 ttcgccgcgc atcgattggt atgtcatcga taaccgcatg c
//
LOCUS       DATAQL010001087         6584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3928774, whole genome shotgun sequence.
ACCESSION   DATAQL010001087 DATAQL010000000
VERSION     DATAQL010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928774"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1887)
                     /locus_tag="VHS97_18745"
     CDS             complement(<1..1887)
                     /locus_tag="VHS97_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3450300.1"
                     /translation="MSSLWQWLLGIRRSSGEVALGDSTRLELTAMPAGAAALALVVAA
                     VALAYVLWRLYRRERRELSRPRRALLVGLRLLVLGALAVMLVEPVLVTSRSETIKSHL
                     PMIVDDSESMRFSDPYTDESRAALVAAALRIGSDGKRSPVERLRESPRLDLARASLGP
                     NLEKLARGRELFVYDLESASRTVGSSRTRQLDELKAVRGVSPLGDALRDVLALHRARP
                     VAGIVLVSDGRSNAGEDPLRAAEAATRQGIPIYLIAAGADEGPRNVRLAEIEASPVVF
                     ARDPMTLGVVVEARGLKDAEGTISLEQRVNGSDWVPVGDQRVALGEDGILKRITFRIV
                     PRVVGDYEFRARVGDAGPELTLDDNIATAAVKVVRQQIRVLLIAGAPSPEVQFLHNAL
                     LRDQHIESAIWMQHVDPGYKQGGDRPIIRLPGDAEELGRYDTLLLVDPDVRALGAQWP
                     ELITNFVGKDGGGLIFVPGELYSQQLFEVDDSESEGAGKWTRLLPVVREAGLFRTEAQ
                     VRLSTQSTYLLELTAEGRGDPIFEFHPDPIKNRAILTSLPGMYWSFPVTRARPGATVL
                     ARHGDPRMQNQHGRHVLLASQLYGPGRTVFLGFDSTYRWRYLSEDYFDGFWARLIDRV
                     GRNKA"
     gene            complement(1924..4086)
                     /locus_tag="VHS97_18750"
     CDS             complement(1924..4086)
                     /locus_tag="VHS97_18750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX3450301.1"
                     /translation="MFTLLSPLLIWGTLLGVIPIIIHLLNRRRFRRVEWAPMRYLKLT
                     IQRNRRRIQIEQLLLLLVRIALPVLLFVLLARPLLNATGLENWLGGSGRVSHVFLVDD
                     SLSMGYAADSQVAFHRAREVVGALLAATRAGDRCTLVTTSASTAPVFQEVEGARREEL
                     AGDALSLPQTETHAAWPAVLAGVDGVLQSCTYPTRELTIITDLRKAGWDAGVSALASR
                     WADQDIPVRVVDVGADEAANIAIESLTPLDRTILAGAESRWEAVVRNASPRLFTGSKG
                     ILRVDDRPTELVLPEIAPGQSVKVPLSVRFPGSGMHELSLQIADDPLPGDNQRWAAVP
                     VKDSLLIRLVDGEPSTEPFGSEVDYMAAPLSIGVGDAEAWRVEVVQEDDFASPRLEPA
                     DVLVLANVAAPTADQANRVAALARAGMGLMIFTGPKLDTGLYNELLYRTGERLLPCSL
                     KAQIDGEIRGLFVEQVRPSPFEKLLELKPSALERVTVRQIMGVDEGPEEAKVRVLARW
                     NDAPRSPAVIERVVGNGRVLLWTTTADRAGSDWPIEPSFVLAVREAVRGTARPTALTN
                     TVSAGEHPRRLIRSNQQVSNVFLSLPGSGEPVPLRAVPIEVSPKDELGPAVAVDLPDT
                     RRAGLYRLALEEGPLGTQKDLFAANPDRRETDLDRIGEKDLKAMLEPLKVEVTRARGE
                     GLDAFSATGQEVWHQLAWGLLALLILEPILATWVGRSR"
     gene            complement(4090..4998)
                     /locus_tag="VHS97_18755"
     CDS             complement(4090..4998)
                     /locus_tag="VHS97_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3450302.1"
                     /translation="MPPSRTYSDPDVIARIADLGLRSRRLVEGAINGQHRSPFHGFNI
                     EFATYREYAPGDDLRRLDWRVFARSDRHYIKQYEEESNARVTFVIDGSASMNYCGAKA
                     DLSKFDYGATLVVSLAMLLARQQDPVGLVLFDEQASKILPPAATQSQVTVMTGLLEKC
                     TPARKTELGGLLQSLSDRIRRRGLLIIVSDLFTDLDAVYDGLNRLRFAGHEVLILQVL
                     DRDETELPFDGPTVFRDIEGDEELFAEPIAFRQAYRRAMQEFLDGVSQECGARGYDHV
                     RFFTDESLGSSLSYFLHSRQLSTGQV"
     gene            complement(4967..6013)
                     /locus_tag="VHS97_18760"
     CDS             complement(4967..6013)
                     /locus_tag="VHS97_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3450303.1"
                     /translation="MSTATEELDLAELDELRTAHHEIREQIARQIVGQDEVIDQLLIA
                     VFARGHCILEGVPGLAKTLMVHSLAQSLSLEFNRIQFTPDLMPSDITGTEVLYEDRTS
                     GARELRFVRGPVFANLILADEINRTPPKTQAALLEAMQERQVTIGGHRHPLPDPFFVL
                     ATQNPIEQEGTYPLPEAQLDRFLFKIFISYPTPDEERRIYRLTTGTDQQEISPLISGD
                     RIGALQHLVRRVPVSDQCIDYAMALVRATRGPAHGGPKYIGDWVSWGAGPRAGQALIL
                     ASKARAALHGRPSVSIDDIRTVARPVLRHRVVINYNAQAVGESSDSIVQKLLADVPVG
                     KGAADAAVADVFRS"
     gene            complement(6030..>6584)
                     /locus_tag="VHS97_18765"
     CDS             complement(6030..>6584)
                     /locus_tag="VHS97_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450304.1"
                     /translation="AAGGWYYIPGSGDEGSVTVTQVQALRAAQNAGFLVPKSVIEEAG
                     KYLEKCRTPEGGIQYSLSFGGGPRLPISAAAVATLYNAGQFDNPIATDCLKYVWGRFQ
                     AHEGWNKGTGHAFYTHLYASQGFYMAGNEYWDKYFPAARDQLIAMQAGDGSWNGDGIG
                     QVYGTAIAVIILQLPFKYLPVFQR"
BASE COUNT         1214 a         2149 c         2076 g         1145 t
ORIGIN      
        1 ggccttgttc cgccccacgc gatcaatcag ccgggcccag aaaccatcga aataatcttc
       61 cgagagatac cgccagcggt acgtgctgtc gaagcccaaa aagaccgtgc ggcccggtcc
      121 ataaagctga gaggccaaga gaacatggcg gccgtgctgg ttttgcatcc ggggatcgcc
      181 gtggcgggcg aggaccgtcg cgccgggacg agcgcgcgtg acggggaagc tccagtacat
      241 tcctggaagg ctcgtcagga tcgcgcggtt tttgatcggg tcgggatgga actcgaatat
      301 cggatcgccg cgcccctccg ccgtcaattc gagaaggtac gtcgattgag tgctcagccg
      361 cacctgcgcc tcggtccgga acagacctgc ctcgcggacg accggcaaca gacgcgtcca
      421 cttcccggcc ccctccgact cggaatcgtc gacctcgaag agctgctgtg agtagagctc
      481 tccgggcacg aaaatgagac cgccaccgtc tttgcccaca aagttggtga tgagctcggg
      541 ccactgggcg ccaagggctc gcacgtccgg gtcaaccagc aagagggtgt cgtagcggcc
      601 gagttcttcg gcgtcgccgg gcagccgaat gatgggtcgg tcgccccctt gcttatagcc
      661 tgggtcgaca tgctgcatcc agatggccga ttcgatgtgc tggtcgcgca acaaggcatt
      721 gtggagaaac tggacttcag gcgacggtgc cccggcgatc aacaggacgc ggatctgctg
      781 ccggaccacc ttgaccgccg cggtcgctat gttgtcgtcc agggtcaact ccggcccggc
      841 gtcgccgacc cgggcgcgaa attcgtagtc gcccaccact ctggggacga tccggaacgt
      901 gatgcgcttc aagatcccgt cctcgccgag cgccactctc tggtcaccga cgggcaccca
      961 atcgctgccg ttgacgcgtt gttccaggga gatggtcccc tcagcgtctt tcaatccgcg
     1021 cgcctcgacg acgacaccca gcgtcatcgg gtcgcgggcg aagaccactg gactcgcctc
     1081 aatctcggcg agacgaacat tccgcggtcc ctcatcggcc ccggcggcga tcaaatagat
     1141 cgggatgccc tggcgtgttg cggcttcggc ggcgcggagg gggtcttccc ctgcgttcga
     1201 ccggccgtcg ctcaccagca caatgccggc caccggccgc gctcgatgaa gcgccagcac
     1261 gtcccgcagc gcatcgccca gcggcgagac gcctcgaaca gccttgagct catcgagctg
     1321 gcgggttcgc gacgatccga cggtccgcga tgccgattcc aggtcgtaaa caaacagctc
     1381 ccgaccgcgc gccagctttt cgagattggg accgagcgag gccctggcca ggtccagccg
     1441 cggcgactcc cgcagccgtt ctacagggga ccgcttgccg tcggaaccga tccgaagcgc
     1501 ggcagccacc agagccgcac gcgattcatc ggtgtatgga tcggaaaatc gcatgctctc
     1561 ggagtcgtca acgatcatcg gcagatgcga ctttatggtc tcgctccggc tggtgacgag
     1621 aactggctcg accagcatga ccgcgagcgc ccccaagacc aagagccgca agcccaccaa
     1681 gagcgcccgc cgcgggcgcg agagctcgcg acgctcacgg cggtaaagcc gccagagaac
     1741 gtacgcgagc gctactgccg ccacgaccag cgcgagggcc gcagcccccg cgggcatcgc
     1801 ggtcagctcc agccgcgtgc tgtcgccgag cgccacctca ccgctcgacc gtctgattcc
     1861 caggagccat tgccagagac tgctcataaa cctttgcctg attccaagtt tttgcgctga
     1921 ccgttaacgc gagcggccta cccaggtcgc gaggatcggc tccaggatca agagggcgag
     1981 cagcccccac gcaagctgat gccagacctc ctgccccgtc gccgaaaacg catccaggcc
     2041 ttcgcctcgc gccctggtca cctccacttt cagcggttcg agcatcgctt ttaagtcctt
     2101 ctctccgatc cgatccaagt cggtctcgcg ccggtcggga ttggccgcga acaggtcttt
     2161 ctgggttcca agcgggccct cttccaaggc gagccggtag agcccggccc ggcgcgtatc
     2221 gggcaggtcg accgccacgg ccgggccgag ttcatctttt gggctgactt caatcgggac
     2281 ggcccgaagc gggaccggtt cgccggaacc cggcagactc aggaagacgt tcgagacctg
     2341 ctgattcgag cggatcaatc tacggggatg ctcaccggcc gataccgtgt ttgtgagcgc
     2401 agtcggccgg gcggtccccc gcacggcctc ccgcacagcc agcacaaagc tgggctcgat
     2461 cggccagtca ctccccgcgc ggtcggcggt ggtcgtccag agcaagaccc ggccgttgcc
     2521 gacgaccctc tcgatcaccg ccggagagcg gggtgcgtcg ttccaccgcg ccagcacgcg
     2581 cacttttgcc tcttcaggac cctcgtccac gcccatgatc tgccggaccg tcacgcgctc
     2641 gagcgccgac ggtttcagct caagcagttt ctcgaacggc gacggccgga cctgttcgac
     2701 gaacaggccc cgtatctcgc cgtcgatctg agccttcaag gaacacggca gcagccgctc
     2761 gccggttcgg tacagcagct cgttatagag tccggtatcg agcttgggcc cggtgaagat
     2821 catcaggccc ataccggccc tggccaaggc ggcgactcgg ttcgcctggt cggctgtcgg
     2881 ggccgcgaca ttcgccagca ccaacacatc agcgggctcg agccgcggcg acgcaaagtc
     2941 atcctcctgg accacctcga cccgccaggc ctccgcatcc cctacgccga tcgacagcgg
     3001 tgcggccatg taatcaacct cggatccaaa cggttcggtc gacggctcac cgtccaccag
     3061 acggatcaag agcgaatctt tgaccggcac ggccgcccag cgctggttat cgccgggcag
     3121 cgggtcgtcg gcgatctgga gcgaaagctc gtgcatacca ctgcccggga accgcaccga
     3181 gagcggaacc ttcaccgatt gtcccggcgc gatttcgggc aagaccaact cggtcggtcg
     3241 atcatccact cgcaggatgc ctttggaccc ggtgaacaat cgcggcgacg catttcggac
     3301 gaccgcctcc cagcggctct ccgcgcccgc caggatggtc cgatcgagcg gcgtcagtga
     3361 ctcgatcgca atgttggcgg cctcgtcggc acccacatcc accacgcgca caggaatgtc
     3421 ctggtcggcc cagcgtgacg ccaaggcgct cacacccgca tcccagccgg ccttccgcaa
     3481 atccgtgatg atcgtcaact ctcgggtcgg gtaagtgcac gattgcagga cgccgtcgac
     3541 acccgccagc acggccggcc acgcggcatg cgtctcggtc tgcgggagcg acagggcgtc
     3601 accggcgagt tcctcgcgac gggccccctc gacttcctgg aagaccggcg cagtcgaggc
     3661 cgaagtcgtg acgagggtac agcgatcgcc ggcgcgtgtc gccgcgagca gagcgccaac
     3721 gacttcgcgc gcgcgatgga aggccacctg actgtcggcc gcgtaaccca tactcagaga
     3781 gtcgtcgacc aagaaaacat gactcacgcg tccggagccc cccagccaat tctccaggcc
     3841 cgtcgcgttc aaaagcggtc gggcgaggag cacgaagagc aagacgggca gagcgatccg
     3901 caccagcaac agaagtagtt gttcgatctg gatcctgcgc cggttgcgct ggatcgtcag
     3961 cttcagatag cgcatcgggg cccattccac cctgcggaac cgtcggcggt tcagcagatg
     4021 gatgatgatg ggaatgacgc cgagcagcgt gccccagatc aataatggac taagaagggt
     4081 aaacatcgtt caaacctgcc cggtcgacaa ctgacgcgag tgcaagaaat aactcagtga
     4141 cgaaccgagt gattcgtcgg tgaagaatcg cacgtggtcg tagccgcggg cgccgcattc
     4201 ttggctcaca ccgtcaagaa actcctgcat tgctcgccgg taggcttgcc gaaacgcgat
     4261 cggttcggcg aaaagttctt cgtccccttc gatgtcacgg aagacggttg gtccgtcgaa
     4321 cggcagttcc gtttcatcgc ggtcgagcac ttgcaagatg agtacctcat ggccggcgaa
     4381 cctaaggcga ttgaggccgt cgtacacagc gtccaggtcg gtgaacaggt cggagacgat
     4441 gatcaaaagg ccgcgccggc ggatgcgatc ggaaagcgac tggagcagtc cgcccagttc
     4501 ggtttttcgg gcaggagtgc acttttccag gagacccgtc atcacggtga cttgcgactg
     4561 ggtcgcggcg gggggcagga tcttgctggc ctgctcgtcg aagaggacca ggccgaccgg
     4621 gtcttgttga cgcgccaaaa gcatcgccag cgagacgacg agcgtggcgc cataatcgaa
     4681 cttcgacagg tcggccttgg cgccgcagta gttcatcgac gcgctgccgt caatgacgaa
     4741 cgtgacccgg gcgttactct cttcctcgta ctgcttgatg taatggcggt cggaccgggc
     4801 gaagacccgc cagtcgagac ggcggagatc atcgccgggt gcatattcgc ggtaggtggc
     4861 gaactcgatg ttgaaaccgt gaaacgggct gcggtgttgc ccgttgatcg cgccctcgac
     4921 aagtcggcgc gaccgcaatc ccagatccgc gatcctggcg atgacgtcag gatcggaata
     4981 cgtccgcgac ggcggcatcg gcggctcctt tcccgaccgg gacatctgcc aggagtttct
     5041 gcacgatcga gtcgctcgac tcgccgacag cctgagcgtt gtagttgatg accacccggt
     5101 ggcggagcac gggccgggca acggtgcgga tgtcgtcgat cgagacgctg ggccggccgt
     5161 ggagcgcggc gcgggccttc gatgccagta tcaaggcctg tccggcacgt ggtccggccc
     5221 cccacgagac ccaatcgccg atgtatttcg gcccgccgtg cgctggcccg cgcgtggccc
     5281 ggaccagagc catcgcgtaa tcaatgcact ggtccgagac tggcacgcgg cgcaccaggt
     5341 gttgcaaggc gccgatccgg tcgcccgata tcaaagggct gatttcttgc tggtccgtcc
     5401 cggtcgtgag tcggtagatc cgccgctctt cgtcgggagt cggataactg ataaagattt
     5461 tgaagaggaa ccggtcgagc tgcgcctctg ggagcggata ggtcccctcc tgctcgatcg
     5521 ggttttgcgt ggccaggaca aagaacgggt cggggagcgg atgccggtgg ccgccgatcg
     5581 tgacctgccg ttcctgcatc gcctcgagca gcgcggcctg ggtcttgggc ggcgtccggt
     5641 tgatctcgtc ggcgaggatc aggttggcaa acactggccc tcggacgaac ctgagttcgc
     5701 gggccccgct ggtccgatct tcgtacagga cttcggtgcc ggtgatatcg ctgggcatga
     5761 ggtcgggcgt gaactggatc cggttgaatt ccagggagag cgactgtgcc agcgagtgga
     5821 ccatcaatgt cttggcgagt ccaggcacgc cctccaagat gcaatgcccg cgggcgaaga
     5881 cagcgatcaa gagctgatcg atgacttcgt cctgtcccac gatctgccgg gcgatctgct
     5941 cgcggatctc gtggtgggcg gttcgcagct cgtccagttc ggcgaggtcg agttcttcgg
     6001 tcgcggtgct caaaatcacg tcctcaattc tagcgctgga agacaggtag atacttaaag
     6061 ggaagctgca agatgatcac ggcgatcgcg gtcccgtaga cctgcccgat cccgtcgcca
     6121 ttccacgaac cgtcgcccgc ctgcatggcg atcaactgat cgcgggcggc ggggaaatac
     6181 ttgtcccagt attcattgcc cgccatgtaa aagccctgcg acgcgtacag gtgggtgtag
     6241 aaggcatgac cggtcccctt gttccacccc tcgtgagcct ggaacctgcc ccagacgtat
     6301 ttgaggcagt cggtggcgat cgggttatcg aactggccgg cgttgtaaag cgttgccaca
     6361 gcggccgccg agatcggtag tcttggtcct cccccgaatg agagtgagta ctggattcct
     6421 ccttcgggag ttcgacattt ttccaggtat ttgccagcct cttcaatcac cgacttgggg
     6481 accaggaacc cggcgttctg ggcggcccgc agcgcctgca cctgggtcac agtgacggag
     6541 ccctcgtcgc ccgatcccgg tatgtagtac cagccgcccg cggc
//
LOCUS       DATAQL010001088         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3013084, whole genome shotgun sequence.
ACCESSION   DATAQL010001088 DATAQL010000000
VERSION     DATAQL010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013084"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VHS97_18770"
     CDS             <1..531
                     /locus_tag="VHS97_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3450305.1"
                     /translation="AAMLMASPRVYYAMARDRLFFPAFAKLDPSRGTPIRAIAIQAIL
                     AVILAASGTFEQILAFLMVPTLAFLVLTVAAVFVLRHDGHAQEPLATPGFPVSPLLFL
                     IPVVTIIILLVLRDPLRASIGLLIVLLGVPISGWVVARSRSRGESVQTRSAGDGNGPP
                     STAQDPSSVSTIGMNS"
     gene            552..833
                     /locus_tag="VHS97_18775"
     CDS             552..833
                     /locus_tag="VHS97_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450306.1"
                     /translation="MAWIRTVPMDDADDSLKKAMADQRALYPAEYSQPVQELDQGLPG
                     IVASHTLIPQALYHAFATFGSLMSPDLPLERRQHEMIATMVSLTNKCHY"
     gene            942..1118
                     /locus_tag="VHS97_18780"
     CDS             942..1118
                     /locus_tag="VHS97_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450307.1"
                     /translation="MVDYVVKLTKDATKISPLDHDRLRGAGFDDRAILQITLIASWFN
                     YINRVADALGVGRD"
     gene            complement(1239..1397)
                     /locus_tag="VHS97_18785"
     CDS             complement(1239..1397)
                     /locus_tag="VHS97_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450308.1"
                     /translation="MALARQGDFLPALEGRLRDGERSGLGVIYLELAMRDDPPRRRTK
                     DAFLPRFR"
     gene            1607..>3718
                     /locus_tag="VHS97_18790"
     CDS             1607..>3718
                     /locus_tag="VHS97_18790"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3450309.1"
                     /translation="MATLAPSAPSTADESHPALERDVLPLLKARCLKCHSPLKSKGKL
                     NLSGPRSLARGGSSGPVIAPGNLDESTLWDLVSNDEMPPRPEEPLSADEKRLLRRWIE
                     QGARDLPTVAEVQGAPPANDHWAFAPATTPPPPSLKDHRRVRTPIDRFIENALEDQKL
                     ALGPEANRTTLIRRLSFDLTGLPPVPEQIAAFVADPASNAYEKLVEGLLASPRYGERW
                     GKFWLDASGYADSNGYFSADTDRPLAYRYRDYVIRAFNADRPLDQIVREQLAGDELAG
                     DRRGPEADPVTIDLLVATHFLRNGQDGTGESDGNPDEVRADKYAVLEGTIQIIGSSLL
                     GLTLQCAKCHDHKFEPVTQQEYYQLQAILYPAFNVEHWVKPNDRIVIAGPRDEMTRWQ
                     VHEKVIDGQLEALERHLASGTNATKSKETLQRVIEAANARRWPNPGRIAWVGDVSNTP
                     SEIPLLVRGNLATPGPKVGPGVLGILTDPENRYVPAAPFAGSSATGRRLALARWLTRP
                     GSRPSALLARVLANRIWQQHFGTGLAATSDNLGYTGSPPTHPELLEWLASELVRSGWS
                     AKALHRLIVTSSVYRQSSAPRPDSASIDPDNRLLSRYSLRRLDAEALRDAILAITGEL
                     NDRQGGPYVPTHRDGSGEVVTESAEGATRRSVYLQQRRTEITSLLDLFDAPSIVTTCT
                     RRVPSTIPLQSLSLMNSDFMVN"
BASE COUNT          702 a         1176 c         1099 g          741 t
ORIGIN      
        1 gctgcgatgc tcatggcttc gccacgcgtt tactatgcga tggcgcggga tcgcctgttc
       61 ttcccggcgt tcgccaaatt ggatcccagc cgaggcactc cgatccgggc tatcgcgatc
      121 caggctatac ttgccgtaat cttggctgcg agcggtacgt tcgagcagat tctagccttc
      181 ctcatggtcc cgaccctggc attcctcgtc ttgaccgtgg cagcggtttt tgtgctccgg
      241 cacgatgggc acgctcaaga accgctcgcg acaccgggct ttccggtctc tccgctcctg
      301 ttcctgattc cggtggtgac gattatcatt ctgctagtac tgagagaccc cctgcgcgcc
      361 tcgatcggct tgctcatcgt attactgggc gtgccgatct cgggctgggt tgtcgcacgt
      421 tctcggtcac ggggcgagag tgttcaaact cgatcggccg gtgacggcaa tggtcctcca
      481 tcgactgccc aagatccgtc ttctgtttcg acaatcggta tgaattccta aaaacgaacc
      541 gaggaaccac aatggcctgg attcgaaccg tgcccatgga tgatgcggac gatagcttga
      601 agaaggcgat ggcggaccag cgcgctcttt accccgcgga atattcgcag ccagtgcaag
      661 agcttgatca agggcttccc ggaatcgtcg cttcgcacac actgattccg caagcgctct
      721 accatgcctt cgccactttc gggtccttga tgtcgcccga cctgccgctt gagcgccggc
      781 agcacgagat gatcgccacc atggtctcct tgaccaataa gtgccattac tgaattgtct
      841 cacacgcaga gtttctgcgt cgggtaacac tggacgatgc tctcatccag gcgcttcgcg
      901 aagattatac gaaggcaccg atcaccgcgg cggagcgggt gatggtcgac tacgtggtga
      961 agctcaccaa ggatgccacc aagatttcgc cgcttgacca cgatcggctg cgcggcgccg
     1021 gatttgacga ccgcgcgatt ctgcagatca cgttgatcgc ttcctggttc aattacatca
     1081 accgcgtcgc cgatgcactc ggggtcggcc gtgactaaac acgccgcctg cgccgatcag
     1141 ccgaccggcg tgggcccatt atcattattc catcgtcacc gcttaggcca ctggagcggc
     1201 acattctgag aaacgtcgat gtgaaagtcg ccactgggtc aacggaaccg gggcaggaac
     1261 gcatccttag ttcgacggcg cggggggtcg tcgcgcatcg ccagttccag gtagatcacg
     1321 ccgagtcccg acctctcgcc gtccctgagg cgaccctcca gcgcgggcaa aaagtcgccc
     1381 tgtcgcgcta gcgccatggc attcaccacg gccgcggccc gttctgcctt tagccagccc
     1441 gtgcgaatac tcgctcctcc ccgccacgct tgggttgtga attattcgga tcattcacga
     1501 taatgagggg cacgggcaag cgcaaagatc ggcgaaccag tgaaacgagg caaccatcga
     1561 acgccgagcc agttcgcgac tgtgttcagt tctggctatt gcactggtgg ccactctcgc
     1621 cccaagcgcg cccagcacgg ccgatgagag tcatcccgcc cttgagcgcg acgtgctgcc
     1681 gctcttgaag gcccgctgcc tgaaatgcca cagtccgctc aaatccaaag gaaagctaaa
     1741 cctgagcggc ccgcgttcac tggctcgtgg ggggtcgagc gggcctgtga tcgcgccggg
     1801 caaccttgat gagagcacac tctgggacct ggtatcgaac gatgagatgc cgccccgacc
     1861 cgaagagcca ctgtcggccg acgaaaaaag actgctgcgg cgctggatcg agcagggcgc
     1921 tagagatttg cccactgtcg cggaggttca aggcgctccg ccggccaatg atcactgggc
     1981 gttcgcgccg gcaacaaccc caccgccccc ctccttgaaa gaccatcgac gcgtccgcac
     2041 tccgatcgac cgctttattg agaacgcact cgaggaccag aagctcgctc tcggtcccga
     2101 ggcgaatcgc actaccttga tccgccggtt gagcttcgac ctgacgggac tgccccccgt
     2161 acccgagcaa atcgctgcat tcgtcgctga tcccgcttcc aacgcctacg aaaagctagt
     2221 cgagggcctg ctggcctcac cgcgctatgg agaacgttgg ggcaagtttt ggctggacgc
     2281 ttctggctat gctgattcca acggatactt cagcgcggac acagaccgcc cgctggcata
     2341 tcggtaccgc gactacgtca ttcgcgcgtt caacgcggat cgaccgctcg atcagatcgt
     2401 ccgtgagcaa ctggcgggtg acgagctggc tggtgatcgg agaggccctg aggccgaccc
     2461 tgtgacgatc gatctgctgg tcgcgactca ttttttgcgg aacgggcagg atggcacggg
     2521 agagagcgac ggtaacccgg atgaggtcag ggcggataag tacgctgtgc ttgaaggaac
     2581 gattcagatc atcggttcgt cgctcttggg actgaccctc cagtgcgcca agtgccacga
     2641 tcacaagttt gaaccggtca cccagcagga gtactatcag ctccaggcga tcctctatcc
     2701 ggcctttaac gttgagcact gggttaagcc caacgatcgg atcgtaatcg cggggcctcg
     2761 ggacgagatg acgcggtggc aggtacacga aaaggtgatc gatgggcagc tcgaggcgct
     2821 cgagcgtcat ttagcatccg ggacgaatgc cacgaagtct aaggagacgc tccaacgtgt
     2881 catcgaggcg gcgaatgccc ggcggtggcc aaatccgggc cgaatcgcat gggtcggtga
     2941 cgtgtcgaac acgccctcgg agattccgct cttggtgcgg ggaaatctcg ccacgcccgg
     3001 tcccaaggtc gggccaggtg ttcttggcat cttgaccgac cccgagaatc ggtatgtgcc
     3061 tgcggcgccg tttgcgggtt cgagcgcgac tggccggcgg cttgccctag cacgctggct
     3121 gacccgtccg ggctcgcggc cgtctgcgct tttggcgcga gtgctggcca atcggatctg
     3181 gcagcaacat ttcggcaccg gactggccgc gacgtccgat aacctcggtt acaccggctc
     3241 gcctcccacg catcccgagt tgctcgagtg gctggccagt gagcttgtgc gctcgggttg
     3301 gagcgccaag gcgttgcacc gcctgatcgt gacttcaagt gtctatcgcc agtcgagtgc
     3361 gccccggcct gatagcgcga gcatcgatcc ggacaaccgc ttgctgagtc gctactctct
     3421 acgacggcta gatgccgagg cactgcggga tgccattctc gccatcaccg gtgagctcaa
     3481 tgaccgtcaa ggcggcccat acgtacctac ccatcgtgac gggtccggtg aggtcgtcac
     3541 ggaatcggcc gaaggcgcaa cccgccgctc ggtctatctc cagcagcggc gcacggagat
     3601 taccagcctg ctcgacctgt tcgacgctcc ctcgatcgtc acgacctgca cccgccgcgt
     3661 gccgtcgacg atccctttgc aatcgctcag cttgatgaat tctgacttca tggtaaac
//
LOCUS       DATAQL010001089         5433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5454384, whole genome shotgun sequence.
ACCESSION   DATAQL010001089 DATAQL010000000
VERSION     DATAQL010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5454384"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VHS97_18795"
     CDS             <1..429
                     /locus_tag="VHS97_18795"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX3450310.1"
                     /translation="DTLIVHQEGAIHLNRTVQEIHRLGLRAGVALNPATPAVLVEEIL
                     PDLELVLVMTVNPGFGGQQFLSGMLPKIRRVRRMIDQVRPECELEVDGGIEPHTAPLV
                     VEAGARVIVAGSAIYGAAGGATAGIARLKEGLSRRAGPIV"
     gene            581..2044
                     /locus_tag="VHS97_18800"
     CDS             581..2044
                     /locus_tag="VHS97_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450311.1"
                     /translation="MNLYYPIQDYGPLMKGMVIGGLGIFHVFLAQMAIGGGMLLCYFE
                     WLRRTGRSRNAARFINGYFQALVLVSFVLGALTGVAMWFTTIQVSPRTIGVLVDEFHW
                     LWATEWTFFCVEVVSGYAFLRYKDRLSARGRMTLLVFYSVAAWFSLFWINGILSFQLT
                     PGRWVQTHDIWAGFFNPSFWPSLLYRTVAAMAIAALAASVVINLANHLEREARRELIG
                     HASVFLAPMVLMPFLGGWFLASMPADSRSWVLGGSVTMTMFMGIGVGASLLIGAYALG
                     AFWYLKLYINGFTAALLCVIALAATAGGEFVREGVRKPFSIRQVLYSNSIRPDEIERL
                     RKVGSVTDDPYPLRDGLTYPNDQLKAGARVFRFQCSVCHTYDGANGLLELTGTWTLDQ
                     KRMNIAELQRTKPFMPPFAGPAPEVEALVQLLSWRRAGMPQSWPVSDDSATLKKIQAW
                     LDEVGTRPGIELMGRRRLATADRPSGDAADATAKERP"
     gene            2044..3165
                     /locus_tag="VHS97_18805"
     CDS             2044..3165
                     /locus_tag="VHS97_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450312.1"
                     /translation="MDAIFPFGFPWPTAMYLTLFIVTAAIYMVFMNYVLAGGIVLLVG
                     YLAPGARRRVAGGPGSTTRSGLGLILKVVGDWLPAVLGVAITTGVAPLLFLQILYKRQ
                     FYTANLLLFNRFMLLLPALIVAYYMLYIVKSHALAARGPMLRGLVAFVAFVCFLYTAW
                     AWTENHVLSLHEEAWKHHYESSNYIYRNAEIWPRLGYYITASFASLAVALAWQLHWGR
                     RFHDPVNLDLASRRLRALALLGLATSAAEAWLWELWLDLPARAVLASSLALPYGLAAL
                     AGMGIQVAGWLTVKTGADMSTRRLALISAGAIMTIVGSLVIREARRLAAIDIAALYDA
                     HRQAGQVGGVSAFLMFFAINAAVITASVLIVRRALRRQN"
     gene            3333..4787
                     /locus_tag="VHS97_18810"
     CDS             3333..4787
                     /locus_tag="VHS97_18810"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX3450313.1"
                     /translation="MSLDVHARQPKLEETVLDSEPSDGPESAEPGTSLTEGGVGVKNR
                     AEPVTAAERFFAVDVLRGCALLGILAMNIVGFGWPEAAYDDPFRGGGFEGLNRGVWFG
                     NHLFFEAKMMTIFSMLFGAGLVLMDQRAEKRGAKIRGIYYRRVLWLLVIGLIHSYLIW
                     WGDILVLYAECGLLLYFFRNLRPRTLIVLGISAMLILVSLILGFAKTIDYMRAASDRY
                     EAQTQAGEKHRGVDHMLHDFWTEDLRDDILPSAEKKAKDWDKEIAVHRGSYLGIVKHR
                     APKLLKTQIFGFLFGGGFFAMSRMLVGMGLMKLGVFSAQRSRKFYLWTVGLGYGIGLP
                     LMVFDAIELIRYKFSFDYMIHGGVLYNLFGSLVVALGHVGLLMLIVQRGAIGWLTTRL
                     AAVGRMALSNYLFDSIVCTALFYGYGFGLFGQINRTGLAAIVLTIWTIQLLVSPIWLK
                     YFRFGPAEWLWRSLTYWRLQPMRQSPEGTAAIAG"
     gene            complement(4874..>5433)
                     /locus_tag="VHS97_18815"
     CDS             complement(4874..>5433)
                     /locus_tag="VHS97_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450314.1"
                     /translation="TLDPGWTFAGTVIGPDDAPLNGARSFGLTDRGWSHEPMNTAEFK
                     VLAFNPRRPRDVFFQHVEMGLVGVMQPPQENGGSVKVQMAPGATIVGRLVEADGTPQA
                     GVKLDLRYRHKMSSMYFEWSEYFPGDIRTSKDGQFHIEALLPGYDFRLSSGKAVVSIR
                     GDTLRSGQTNDLGAVKIDVNGDQHQ"
BASE COUNT          937 a         1586 c         1689 g         1221 t
ORIGIN      
        1 gatactctga tcgtccacca ggaaggggcg attcacttga atcgcaccgt gcaggagatt
       61 catcgattag gcttacgggc gggagtggcc ttgaacccgg cgacgcctgc tgtgctcgtc
      121 gaggagatat tgcccgacct cgagctggtg ctggtgatga ccgtcaaccc tggcttcggc
      181 ggtcagcagt tcctctccgg catgttgccc aaaattcgcc gcgtccggcg gatgatcgat
      241 caggttcgcc ctgaatgcga gctggaggtc gacggcggta tcgagccgca tacggccccc
      301 ctggttgtcg aggctggcgc gcgggtcatc gtggccggct cagccatcta cggcgccgcc
      361 ggaggtgcca ctgcgggcat cgcccggctc aaagagggac ttagccggcg agcaggaccg
      421 atcgtttagc cggcggaaac aggttgcgac gcggcacttc aagttttcct ttctcctttt
      481 tcaagtccac aagcatcgtg cccaggcgac cgctttcctg ggttcggtct tcttttagaa
      541 tcaactctgc tcaatcgtcg ttccgcacat tggaattctc atgaatttat attacccgat
      601 ccaggattac ggccccctaa tgaaagggat ggtgatcgga gggttgggaa tatttcatgt
      661 ttttctggca cagatggcga tcggcggcgg tatgctgctt tgttatttcg agtggctgcg
      721 gcgcaccggc cgaagccgta acgcggcgcg gttcatcaac ggctacttcc aggccttggt
      781 gctggtcagc tttgtattgg gggccttgac tggtgtggct atgtggttta cgaccatcca
      841 ggtcagcccg cgcacgatcg gggtgttggt cgacgaattc cactggctct gggccaccga
      901 atggacgttt ttctgcgtgg aggtggtctc gggctatgcc tttctgagat acaaggatcg
      961 actgagtgca cgaggccgga tgacgctgct cgtcttttac tcggtggcgg cgtggtttag
     1021 cctgttctgg atcaacggca tcctgtcgtt ccagctcacg cctggccgct gggtgcaaac
     1081 gcacgatatc tgggctggtt ttttcaaccc gagcttctgg ccgtcgctcc tctaccgcac
     1141 ggtggcggcc atggcgatcg cggcgctggc ggcatccgtg gtgattaacc tcgcaaacca
     1201 tctggagcgc gaggctcggc gcgagctgat cggacatgct tcggtctttt tggcacccat
     1261 ggtcttgatg cctttcctcg ggggttggtt tctggccagc atgcccgccg atagccgctc
     1321 gtgggtactg ggggggagcg tgacgatgac catgttcatg gggatcggcg tgggcgcatc
     1381 actcttgatt ggcgcgtatg ccctgggtgc attctggtat ctgaaactct acatcaacgg
     1441 cttcacggcg gccctcttat gcgtgatcgc attggcggcc acggcgggtg gcgagtttgt
     1501 ccgcgaagga gttcgcaagc ccttttcgat ccgtcaggtc ctgtactcca actcgatacg
     1561 cccggacgag atcgagcggc tgcgcaaggt cggctcggtc accgacgacc catatccact
     1621 gcgcgatggg ctgacttatc ccaacgatca gctcaaggcg ggtgcacggg tcttccgctt
     1681 ccagtgcagc gtctgccata cgtacgacgg ggccaacggc ttgctcgagt tgaccgggac
     1741 ctggactctc gaccagaaac gaatgaatat cgcggaattg cagcggacca agccattcat
     1801 gccccccttt gcgggcccag cccctgaggt agaagccttg gtgcagcttt tgagctggcg
     1861 acgcgccggg atgccgcaga gctggccggt ctcggatgat tccgccacgc tcaagaagat
     1921 ccaggcctgg ctcgacgaag tgggcaccag gcccggcatc gagctgatgg gtcggcgccg
     1981 tctcgcaaca gccgacaggc cctctggcga cgcagccgac gcgaccgcaa aggagcggcc
     2041 ttgatggatg cgattttccc attcggattt ccgtggccaa cggccatgta tctaaccctg
     2101 tttatcgtca ccgcggcgat ctacatggtc ttcatgaatt acgtgctggc cggagggatc
     2161 gtgcttttgg tcggctatct ggcgcccgga gcccggcggc gagtggcggg gggaccgggc
     2221 agcacgactc ggtcgggcct gggtctgatc ctcaaggtgg ttggcgactg gctgcctgcc
     2281 gtcctcggcg tggcgatcac cactggcgtc gccccactcc tgtttctgca aatcttgtat
     2341 aaacgccagt tctacacggc caatttgctg ttattcaatc gattcatgct gctcttgccc
     2401 gcgctcatcg tagcatacta catgctctac atcgtcaaga gccacgcact ggccgcgcgg
     2461 ggcccgatgc tgcgcgggct ggtcgcgttc gtggcgttcg tctgttttct ttacaccgca
     2521 tgggcatgga ccgagaatca cgtcttgagc ctgcatgaag aggcctggaa gcatcactac
     2581 gaatccagta attatatcta tcgcaatgcc gagatctggc cgcggctggg ctactatatc
     2641 accgcgtcat tcgccagcct ggcggtcgcg ctcgcctggc agcttcattg gggtcggcgg
     2701 tttcacgatc cggtcaatct ggatctcgct agccggcggc tgcgcgcgct ggcgctactg
     2761 ggtttggcga catcggcggc cgaggcctgg ctctgggagc tttggctgga cttaccggca
     2821 cgtgcggtcc tcgcgagcag cctggcgttg ccgtacggtc tggccgcttt ggcgggcatg
     2881 gggatccagg tcgccggctg gttgaccgta aaaaccggcg ccgacatgag cacgaggcgg
     2941 ctcgcgctga tctcggccgg agcaatcatg acaatcgtgg ggagccttgt gatacgcgag
     3001 gcgaggcgtc tagcggcaat cgacatcgcg gcgctgtacg acgcgcaccg gcaagcaggg
     3061 caggtgggcg gcgtcagcgc tttcctgatg tttttcgcga tcaatgccgc ggtgatcacg
     3121 gcctcggtct tgatcgtccg gcgtgcgcta cgtcgacaaa actgaggtcc ctcttacggc
     3181 catcgccgtt cgccgctaat attcccggac ggttgccgaa agacatgtta aattcggatc
     3241 ccttttctcg ctttttcgtt agcggtagaa ggcggttggc tctttgatct tagttcttgg
     3301 ctcttcgatc ctacttcaag gacgttcgga caatgtcgct cgacgtgcat gcccggcaac
     3361 cgaaactgga agagaccgta ctcgacagtg aaccgagcga tgggccggag tcggcagagc
     3421 ccgggacatc gttgacggag ggaggagtcg gggtcaaaaa ccgtgccgag ccggtcacgg
     3481 cggccgagcg attctttgcc gtggacgtgc tccggggctg tgcgcttttg ggcattttgg
     3541 cgatgaatat cgtcggtttt gggtggccgg aggccgcata tgacgatccg ttccgcggcg
     3601 gcggcttcga ggggcttaac cgcggtgtct ggttcggcaa tcacttgttc ttcgaagcca
     3661 agatgatgac gatcttctcc atgcttttcg gcgcggggct ggtgctgatg gaccagcggg
     3721 ccgagaagcg cggggcaaag atccgcggaa tttattaccg tcgcgtcctc tggctgctgg
     3781 tgatcggcct catccactcc tatctgatct ggtggggcga tatcctggtg ctctacgccg
     3841 agtgcgggct gttgctctac ttcttccgca atctgaggcc ccggacgctg atcgtcctgg
     3901 gaatctcggc catgctgatc ctcgtatcct tgatcttggg cttcgccaag accatcgatt
     3961 acatgagggc cgcgtcggat cggtacgagg cccagaccca ggctggcgag aagcaccgcg
     4021 gagtcgacca catgctgcac gacttctgga cggaggatct tcgggacgac atcctgccca
     4081 gcgccgagaa gaaggccaag gattgggata aagagattgc cgtccatcgc ggcagttatt
     4141 tggggatcgt caagcatcgg gccccgaagc ttctgaaaac ccaaatcttt gggttcctct
     4201 tcgggggtgg attcttcgcg atgagccgga tgctcgtggg tatgggcttg atgaaactcg
     4261 gggtcttttc cgctcagcgg tcgcgaaaat tctacttgtg gacggtcgga ctgggctacg
     4321 ggatcggcct gccgctgatg gtcttcgacg ccatcgagct tatccggtac aagttctcgt
     4381 tcgattacat gatacacggc ggcgtcttgt acaacctatt cggcagcttg gtcgtggcct
     4441 tgggccacgt gggactgctc atgctgatcg tccaaagagg cgcgatcggt tggctcacca
     4501 cccggctggc cgcggtcggc cgcatggcac tctccaacta ccttttcgat tcgatcgttt
     4561 gcacggcgct tttctacgga tatggctttg gcctgttcgg ccagatcaac cgcacggggc
     4621 tagccgcgat cgttctgaca atctggacca tccagctact tgtcagcccg atctggctga
     4681 aatacttccg gttcggccca gcggaatggc tctggaggtc gttgacttac tggcggctgc
     4741 aaccgatgcg gcagagtccg gaggggacgg cggcgatcgc aggttgatcc cactcgcgag
     4801 ttagccttga tcatcgcggc aaggcttgtt tcgacgagcg caaacgaact atctcttcga
     4861 aaccaacctc atcctattgg tgctgatcgc catttacgtc aatcttcacg gcacccaggt
     4921 cgttcgtttg gcccgagcgg agagtatcgc cccgaatgga caccacggcc ttgccggagg
     4981 acagacggaa gtcgtagcca ggcagcaaag cctcgatgtg gaactgcccg tccttgctcg
     5041 tccggatgtc tccgggaaaa tactctgacc actcaaagta catcgagctc atcttatgac
     5101 ggtagcgaag atccaatttg acgccagcct gaggagtgcc atccgcctct accagccggc
     5161 cgacgatcgt cgcgccgggc gccatttgca ccttgaccga accgccattt tcctgcgggg
     5221 gttgcatcac cccgaccagc cccatctcca cgtgctgaaa gaacacatcg cgcggtcgtc
     5281 gcgggttgaa cgcaagcact ttgaactctg ccgtgttcat cggctcatga gaccaaccgc
     5341 ggtccgtcag tccaaagctc cgtgccccat tgaggggtgc gtcgtccggc ccgattaccg
     5401 tgccggcgaa cgtccagccg gggtcgagcg tca
//
LOCUS       DATAQL010001090         5129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3013511, whole genome shotgun sequence.
ACCESSION   DATAQL010001090 DATAQL010000000
VERSION     DATAQL010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013511"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            832..1236
                     /locus_tag="VHS97_18820"
     CDS             832..1236
                     /locus_tag="VHS97_18820"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3450315.1"
                     /translation="MSPSERKDLSKAEWKVMKIVWELQKAMAREVYAIAGEQHAWTPA
                     TVKTLLKRLVDKGYVSTTRVGNGFVYRPAQSALATLQSAADTLMTNAIGGVTGPLLVH
                     MVEQTPLSEADLDSLQKLIDAKKQSLKKRRDR"
     gene            1270..4797
                     /locus_tag="VHS97_18825"
     CDS             1270..4797
                     /locus_tag="VHS97_18825"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX3450316.1"
                     /translation="MMNVMIAHANAAGARWGAWVVAASLDAAFLLALLSLVWLAIRRR
                     FVPQLGYCLFLLVPLKLLVPVVVTVPSAIAEWTPSALASSWFDRARVPVTIAFQPKVA
                     RQIASVQPEMAAPSEPRHESLSLSTPITANAQERAIPHQPRSWVRAESTASALTDRVS
                     DMARLSLPAIAMLAWICVITMLFARLALMQVRFRAAIRRVSLQHELTLPIDMRELCRR
                     AGVDRMVRAMEADSVAAPSVWGITRPTIILPRGIAAVLSVRQLQWVVLHELSHIRRGD
                     LIVVILQRFVAILYFFNPAIWMANRIIDQLREYACDDLAVTFGDCSAIESCEAFIHVL
                     RHARHQCGSLGVSLGIFGLSSKSSYYHRVRRLLEIDKPIHTAPGVWSVCSLMVLAVVS
                     VPHLRSANVPVIESALATEGQEFALDVVGPTGKPIPAAQVELQTDLALTVEQVINGQF
                     VKQESSGILAATNTDGRLVVRLPRTPSHFNVKIISPGYGPFWAGWSSENHIESIPSRL
                     VAQLEPGYSVGGIIIDAAGEPVEGATIVPRVESKKPPGDLRQFIVGTRVTTNAAGRWR
                     FDSVPLSMTELPVIVKHPLFIPARWRLTRGEFGIEGPQQPESKLVLERGLTVIGTVTD
                     EGGQPIAGAMIHTNYVNGISGARSTKTASDGTYILAGFAPSATRIVVTAKGRALDMRD
                     LDITSGMGPVNFQLKAGGTVRIRVLDEQGKQVPNARIFFQRWRGRIRHFEFDHLNHYT
                     DDNGVWVWNEAPLDEFHADICPPDGMRLSAQRLIAREQEYVYRVSAPLVVSGKVVDAE
                     TKQPIKTFRVVPGFRSSQSPVYWDGSGSFTASDGQYRIRHRLGYSAHLVRIEADGYRS
                     AFSRDIASNEGTVAIDFELKTGKNIVAKVVTPRNAPAAEANVVLAGAGSHIRMSDGAI
                     DGGLAYCTRAIADETGLFHFPPQDTNFKLVISHPSGYAEFNSSPEWDLTRIIHLEPWA
                     KVEGTFRVGQVPVTNATISINLDSRPHVLGDDGPDVLAQYFTTTGPGGRFVFDRVIPG
                     RGRIGRLLMPSVADGAMSMDSSCRLATNFVGGQTVEIGLNGSGRPVVGKLLPPEGFAE
                     SVRWGLVQVRVQPLAAEIRGINPQWTATVDREGNFRIDDMPAGDYALSVSFQRDDAGH
                     LRNYRFRVPPQEGVRAAEPLDLGPLKLTR"
     gene            complement(4922..>5129)
                     /locus_tag="VHS97_18830"
     CDS             complement(4922..>5129)
                     /locus_tag="VHS97_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450317.1"
                     /translation="IRGIFSLIGRKLDLVAEYEDRVPAYVEQMFDRLEASGIILGWDR
                     NDWEIWTTQIGLDIRIEPKPPAGS"
BASE COUNT         1048 a         1508 c         1491 g         1082 t
ORIGIN      
        1 cgaggctctg tccgagccac ttcgggatgc cggctcgggc ggagcctcgc cctcccgggg
       61 aaaacgtcgc ttttcacccc gcgaaaggta taactctcag cgtcttttgc tgataaaaga
      121 gcttcgacca taacgaccca acaacttcgc aacgtttacg attcccggcc tttccagaca
      181 ttcgctatgc atctggccga cggccggaca attccggtcc gccaccgcgc ggacagcgca
      241 tcgcgggggc acagaacgtt tttgggcagc cacttcgatc gccagaatcc gttggcgcac
      301 cggctttcgg ttggactctc atacttcgtt tccttagttt ccttctgttc gaggcttgtt
      361 agcaccgggc tggacaagcc ttgaaattcc gtcaacaagc ttgacctcat gaaagttgaa
      421 caagggcccc agaggcacgt tcacgagttt gatactctcc aacagtccag ggcccatgat
      481 ccggcgcact aggagagcct gtcagctctg acgaatagcg agtgctttgt gtttttctca
      541 tcttggaaca gaaggaaacg aagaaaacaa agaaatccaa gatcggaaat cggagcgcat
      601 gtgattttcc gcgatacgtg aagtctccgg catcgcagtg gctcagagcg ttttggggca
      661 agcaactcga tagccggaat ccgctggcgc acccgccaag ctgattcgta gcgcgcagtc
      721 gttgacaatt gtactcgatc cgattacagt gctcgatgtc tgacgccaca agttcgagct
      781 gtgccatttt gcattcacgg attcttgagc aacggatcgt gaggcggatc gatgagtccc
      841 tcggagcgga aggatctttc caaggcggaa tggaaagtca tgaagatcgt ttgggagttg
      901 caaaaggcga tggcgcgcga agtgtacgca atcgccggtg agcagcacgc ctggacgccg
      961 gcgaccgtca aaaccctgct caagcgattg gtcgacaagg gctatgtgtc caccacgcgg
     1021 gttggaaatg ggttcgtgta ccggcccgcc cagtcggctc tggcgacact tcaatcggcc
     1081 gccgacactc tcatgaccaa cgccatcgga ggcgttacgg gccctctcct ggtgcacatg
     1141 gtcgagcaaa ctccactgag cgaagccgac ctcgactcgc tccagaagct catcgacgcc
     1201 aaaaaacagt cgttgaaaaa gcgccgcgac cgataggttg acagtgtgtt ctgcaaggag
     1261 gacacggtga tgatgaatgt catgatcgca catgccaacg ccgcaggtgc tcgttggggg
     1321 gcatgggtcg tggccgcgtc gcttgatgcg gcttttttgc ttgcgctcct gagcctggtg
     1381 tggttggcga tacgccggcg attcgtgccg cagttaggct actgtctctt tcttcttgtc
     1441 ccactcaagc tgttggtgcc ggtggtggtc acagtcccgt ccgccatcgc ggagtggacc
     1501 ccttcggctc tggcatcgtc gtggttcgat cgagctcgtg tccccgtcac gatcgcattc
     1561 caacccaagg tcgcgagaca aattgcctcg gtccagccgg aaatggcagc accatccgag
     1621 ccgagacatg aatccctctc gctttcgacg cccatcacgg caaacgccca agagcgggcg
     1681 attccacacc aaccgcgttc ctgggtgcga gcggagtcga cggcgagtgc tttgactgac
     1741 cgcgtctccg atatggcgcg cctgtcgtta ccagcaatcg cgatgttggc ctggatttgc
     1801 gtgatcacga tgctgtttgc gcggcttgcc ctaatgcaag ttcgatttcg agcggcgatc
     1861 agacgcgtct ctcttcaaca cgagcttacc ctccccatcg acatgcgcga actgtgtcgc
     1921 cgcgccggag tcgatcggat ggtccgtgct atggaagcgg attccgtcgc ggcgccgtcg
     1981 gtctggggaa tcacgcgacc aacgatcatc ctacctcgag gaatcgccgc tgtgctgtcc
     2041 gtacggcaat tgcaatgggt cgtgctgcac gagctctctc atatccggcg cggcgacctg
     2101 atcgtcgtca ttctacagcg atttgttgcc atcctctact tcttcaatcc ggcgatatgg
     2161 atggctaatc gcatcattga tcaacttcgg gaatacgcct gtgacgacct tgcagtcaca
     2221 tttggcgatt gttccgcgat cgagtcctgc gaggcgttca ttcatgtttt gcgccacgcg
     2281 agacatcaat gcggcagtct cggcgtatct ctcggcattt tcggactaag ctcgaaatcg
     2341 tcatattatc accgtgttcg ccgcttgcta gaaatcgaca agccgataca caccgcgccg
     2401 ggtgtgtggt cggtttgcag cctgatggtc ctcgccgtcg tgtcggtgcc gcatttgcgc
     2461 tccgcaaatg tgccggtaat tgagagcgcc ttggccacgg aaggccagga attcgccctg
     2521 gatgtggtgg gtccaacagg caagcccata cctgcggccc aagtagagct gcaaacagac
     2581 ctggcactga cggtcgagca agttatcaat ggccagtttg tcaagcagga atcttccggc
     2641 atattggcgg cgacaaatac ggacggccga ctggtggtca ggctgccgcg aacgccgagc
     2701 catttcaacg tgaagatcat cagtcccggc tatggaccat tctgggctgg ctggtcatcc
     2761 gaaaaccaca tcgagtcgat tccgtcccgt cttgttgctc aactggagcc gggctattcg
     2821 gtcgggggaa tcattatcga cgccgcggga gagcctgtcg aaggcgccac gatcgtcccg
     2881 cgcgtcgaat ccaaaaaacc tcccggcgac cttcgacagt ttatcgttgg cacgcgagtg
     2941 acgaccaacg ccgctgggcg atggcgcttt gacagcgtgc cgttatccat gaccgaactc
     3001 cccgtgatcg tcaaacaccc cctattcata ccggctcgct ggaggttgac ccgcggcgaa
     3061 ttcggcatcg agggcccaca gcaaccggaa agcaagctcg ttctggagcg cgggctgacc
     3121 gtaatcggca ctgtgaccga tgaaggagga cagccgatcg ccggagcgat gattcatacg
     3181 aactatgtca acggcataag cggtgcccgt tctacgaaga cggccagtga cggcacttac
     3241 atactggccg gcttcgcgcc gtcagccacc cggatcgtgg tcactgccaa ggggcgtgcg
     3301 ctcgacatga gagaccttga tatcacttcg ggaatgggac cggtcaactt ccagctgaaa
     3361 gccggtggaa cagttcgaat ccgcgttctc gatgagcaag gcaagcaggt tcccaatgcg
     3421 cgcatcttct tccagaggtg gcgcggccgc attcggcact ttgagttcga tcacttaaac
     3481 cactacaccg acgacaacgg cgtgtgggtc tggaacgaag cgcctctcga cgaattccac
     3541 gcggacattt gcccgccgga cggcatgcgg ttgtccgcac aaaggctgat cgcccgcgag
     3601 caggaatatg tctatcgagt atccgctccg cttgtcgtgt cgggaaaggt cgtcgacgcg
     3661 gagacaaagc agcccatcaa aacgtttcgg gtggtcccgg gattccgttc cagccaatca
     3721 cccgtctact gggacgggtc tggaagcttt acggcctccg acgggcagta tcgaatccgc
     3781 catcggctcg ggtattccgc gcatctggtt cgcatcgaag ccgacgggta tcgatctgcc
     3841 ttttcgcgag atatcgcgag caacgaaggg actgtcgcga tcgatttcga gctgaagaca
     3901 ggaaaaaaca tcgtcgcgaa agtcgtcacg ccgcgcaacg ccccggcggc tgaagcgaat
     3961 gtcgtgcttg cgggcgccgg ctcgcacatt cggatgtctg acggcgcgat cgacggcggg
     4021 ctggcatact gcacgcgagc aatcgccgac gaaaccggcc tcttccattt cccgccgcag
     4081 gacaccaact tcaagctcgt catcagccac ccttccggct atgcggaatt caattcgtcc
     4141 cccgaatggg atttgacgcg gatcattcac cttgaacctt gggccaaagt ggaaggaacc
     4201 tttcgcgtcg gacaggtgcc ggtgacgaat gcaacaatat cgatcaatct ggatagccgc
     4261 ccgcacgtac tcggagacga cgggccggac gtgctcgctc aatacttcac gactaccgga
     4321 ccgggaggac gattcgtgtt cgatcgcgtg attccgggcc gtgggcggat tggtcgactc
     4381 ctcatgccga gtgtggccga cggcgccatg tccatggatt cgtcttgcag gctcgcgaca
     4441 aacttcgtcg gcgggcagac cgtcgagata ggacttaacg gcagtggtcg cccggttgtc
     4501 ggcaagctgc tgccgcctga aggatttgcg gaaagtgttc gctggggtct cgttcaggta
     4561 agggtccaac ccctagcggc tgaaatacgc ggaatcaatc cacaatggac ggccacagtc
     4621 gatcgcgaag gtaacttccg tatcgacgac atgcctgcgg gcgactatgc gctgagcgtt
     4681 tcgttccagc gggatgacgc cggccatcta cggaattatc gtttcagagt cccgccgcag
     4741 gagggggttc gcgcggcaga gccgctggat ctgggaccac taaaactaac ccgttgatga
     4801 cttggtctca atttggatgt ccagcccgat tgggtccaag ctgaagttgc cgcgactcgt
     4861 cgacggccgc ttgtcgatta tccattgctc tccttcagtc gagtttgttg gacagccgcg
     4921 atcatgaacc ggcgggcggc ttgggctcga ttcggatatc tagcccgatc tgagtcgtcc
     4981 agatttccca gtcgttgcgg tcccagccga ggatgatgcc cgaagcctcg agccggtcga
     5041 acatctgttc cacataggcc ggcacgcgat cctcgtattc ggcaaccagg tcgagcttgc
     5101 gaccgatcaa gctgaaaatg ccacgaatc
//
LOCUS       DATAQL010001091         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4081036, whole genome shotgun sequence.
ACCESSION   DATAQL010001091 DATAQL010000000
VERSION     DATAQL010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4081036"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VHS97_18835"
     CDS             complement(<1..522)
                     /locus_tag="VHS97_18835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3450318.1"
                     /translation="MSADSQKYIPGFDGLRALAVAAVMACHLDFHRLSQGWIGVPLFF
                     VLSGFLITGILLDAKGSPEYFRRFYSRRTVRILPIYYLLITTIFAIGIVRHWKVFDVS
                     LFATFVDNWKLGVYGPTSGRVGFPPFMYHLWSLAVEEQFYLIWPVAIIALSRRAFVVV
                     VGALIVSGILFRSL"
     gene            complement(575..>1669)
                     /locus_tag="VHS97_18840"
     CDS             complement(575..>1669)
                     /locus_tag="VHS97_18840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3450319.1"
                     /translation="RLDLHVFFGQTLTPAQQASAGFNTPFDWDVDLLSGYESTFLTNI
                     ARQPGPGHFFGSDTPEIWARLNEGGFDAVLVLGWYLKAFVQATWAAKRLGLPVLVRGD
                     SQLATPRGPATRMAKAFAYPPLLRVFDAALYVGQRSRAYYEHYRYPARQLFFSPHCVD
                     TTWFAARATAEAGQTLRTRLGVTREDKVVLFAGKLLSFKRPLDVVEACAGMAGVHVVI
                     AGSGELESALRERACALDVPLHALGFLNQTQMPAAYAAANVLALPSTGRESWGLVANE
                     ALACGTPIVVSDAVGCAPDLAADGAAGRVFPLADVAALRRMIREVLDDPPGRDATAAK
                     SDAYGLGAAVAGVEQALVGSRARRRASPWL"
BASE COUNT          364 a          515 c          529 g          261 t
ORIGIN      
        1 caacgagcga aagaggattc ctgacacgat cagggcgccc acaacaacaa cgaatgctcg
       61 ccgagacaaa gcgatgatcg ccaccggcca tattaggtag aactgctcct ccaccgccag
      121 cgaccaaagg tggtacatga aggggggaaa gccaacgcga ccgctggttg ggccatagac
      181 gcccaacttc cagttatcca caaaggtggc aaagagggaa acgtcaaata ctttccaatg
      241 tcgaacaatc ccaattgcga agatggtcgt aatgagtaaa tagtatatcg gtaggattcg
      301 tacggtccga cgagaataaa accgcctaaa atactctgga gagcctttag cgtcgagcaa
      361 gataccggtg atgagaaaac cacttagtac gaagaatagc ggcactccaa tccaaccttg
      421 acttaggcgg tgaaaatcta aatgacacgc cataaccgcg gcgaccgcaa gtgcgcgcaa
      481 gccgtcaaat cctgggatat atttctgaga atctgcagac atcctcacaa ccttatcggt
      541 ttattgagct tcagagaaat tgcctcgccg gtcgctagag ccatggcgac gcccgccgcc
      601 gagctcggga gcccaccaag gcctgttcga ctcccgccac ggcggcccca aggccgtagg
      661 cgtcggactt cgccgctgtc gcgtcgcgtc ctggcggatc gtcgagcacc tccctgatca
      721 tccttctcaa cgcggcgacg tccgcgagtg gaaacacccg tccggccgcg ccatcggcgg
      781 ccaggtccgg cgcgcaaccg acggcgtccg agacgacgat cggtgtcccg caggccaggg
      841 cttcgttggc caccaggccc cagctttcgc gtccggtgga gggcaatgca aggacgttgg
      901 cggcggcata ggcggctggc atctgggtct gattgagaaa gcccagggcg tgcagcggca
      961 catcgagcgc gcatgcccgc tcgcgcagag cactctccag ttcgcccgac ccggcgatca
     1021 cgacatggac cccggccatg ccggcgcagg cctcgaccac gtcgagtggt cgcttgaagg
     1081 acaggagctt gccggcgaac agcacgacct tgtcctcccg ggtgaccccc agccgcgtcc
     1141 gcagagtctg acccgcctcg gcggtggcgc gcgcggcgaa ccatgtcgta tcgacgcaat
     1201 gcggcgagaa gaacagctgt cgcgccgggt agcggtagtg ttcgtagtag gcgcgggagc
     1261 gctggccgac atagagggcg gcgtcaaaaa cgcgcagaag tggcgggtag gcgaaggcct
     1321 tggccatacg ggtcgcgggc ccgcgcggcg tcgccagctg gctgtcgcct cgcaccagga
     1381 ccgggagccc gagacgcttg gccgcccagg tggcctggac gaaggccttg agataccagc
     1441 ccagcaccag cacggcgtcg aacccgcctt cgttcagccg ggcccagatc tcgggcgtat
     1501 cgctgccgaa gaaatgtccg ggacctggct ggcgggcgat gttggtcagg aaggtcgatt
     1561 catagcccga gagaaggtcg acgtcccagt cgaacggcgt gttgaagcca gcgctggcct
     1621 gctgcgcggg cgtaagggtc tggccgaaga acacgtgcag atcgaggcg
//
LOCUS       DATAQL010001092         5065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_111910, whole genome shotgun sequence.
ACCESSION   DATAQL010001092 DATAQL010000000
VERSION     DATAQL010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111910"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1718
                     /locus_tag="VHS97_18845"
     CDS             90..1718
                     /locus_tag="VHS97_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450320.1"
                     /translation="MGQGANSDRFLMKAMREAGLGRPTRPVVALLAMMILAAPIWLMS
                     DQLRGFALQPTDFVYIQQSRDARALRQNLMAPLNTHVVPFFRLWTYALLSTAGSLEAL
                     PPVFSAASYFSLVVAMVLIGLLVQQETGRAAVALSAMACLGSSTVMLPAVCHYAAGQA
                     LWAGAGAVATLLAATGWRTKGGAWRFGLAWLGVIASPFIWSGGLTAGPAVAAALWADG
                     RPRCRRAAALMAVTTCVVGAAIAWASRDYIRHTPMVWERHSELWPRPVQAVLHSSQAI
                     AEVLIFRNLGVDAETSPFQATVIVACLAGLWYASRGRFSRATPLEAAGAAVVTVSVLM
                     TYFFRGNLPYRSVRPVLWYYLIPQVGAVLLAAGWWVGIGRVARCTGEELTWGGLLAVL
                     TLAFVLLKVQAVRAERILIEDAAPLAPSEFTKFPLTKLKRLRAIYLNHESAKLQRRML
                     ARLDRVEQIARRKGIGKAAIRQTFGRVLVPGLPADLQPYDAAGMLSLPEGDPNLSPML
                     VSSALADYYWEEAAPRPPWLLITDPWPVDAGDRR"
     gene            complement(1776..2252)
                     /locus_tag="VHS97_18850"
     CDS             complement(1776..2252)
                     /locus_tag="VHS97_18850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX3450321.1"
                     /translation="MKSLTLVIAAVVSVGLFIAAGDPRPGAPDATKEGDEKTIRALLH
                     DLGEEWNKHEMKEFTARMGENADAVNRFGQWMKGRAAIREHLITLHGSPIRPMLESRS
                     SKIEQVRFLTPDVALAHELTDEKTGKSVRTYVLQKRDGQWWIESADIVQQGQALHP"
     gene            complement(2340..3035)
                     /locus_tag="VHS97_18855"
     CDS             complement(2340..3035)
                     /locus_tag="VHS97_18855"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3450322.1"
                     /translation="MAKTRRLSSEERRDSILKAARRVFAENGFRGTTTKALAEAAGVS
                     EALLFQHFPTKEALYTAMLSALFGEHEPDVNSQLVAMEPSTATLVLIVHQFYKSLIDP
                     RGRADKADVANIARLMFRSVVEDGEFARLFIQRIPSRMVAKLEECIKAAVVAHDLNVS
                     HSHFSIQGWFTHHLAIILMLHQLADPPIVDYGVSKATLIEEAVLFALRGIGLKEKAIR
                     RYLVPKKSSATQY"
     gene            complement(3229..4587)
                     /locus_tag="VHS97_18860"
     CDS             complement(3229..4587)
                     /locus_tag="VHS97_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450323.1"
                     /translation="MLSILGQKSAAYCDGHSRRHFLKIGGLAMGGLALPQILKAQTLT
                     DSLGPRTRSHKAVIMIYLSGGPSHQDMYDLKMNAPAEIRGSFRPIPTNVPGIEICEHM
                     PRLAKMMDKFTIIRSLYGSPDQHNSDMCLSGYPKGPRGNQDGHPSLGSVISKLKGPID
                     PAIPPSIGLTIKTKHLPYSNPGGPGFLGMAHSPFLPDGPEMTEMRLHGVTLDRLRDRR
                     ILLASYDSFRRRTEQQAISQGLDDFTQAAFEVLTSNKLAEALDLEREDPKLRDRYGRG
                     SADPAFGEDAGPHWMDQFLLARRLVEAGARCVTLSFGSWDRHGGNFAKLPPQLDKFDQ
                     GITALVEDLHSRGLDQDVSVIAWGEFGRTPRINKSAGRDHWPKASCALLAGGGMRMGQ
                     VIGSTNRLGEEPQDRPVHYQDVFATLYHQLGIDVQNTMIYDATNRPHYLLDRRDPIRE
                     LS"
BASE COUNT          920 a         1461 c         1624 g         1060 t
ORIGIN      
        1 cccgcaaaaa gtcgcgcgac caccgggaag ttgttccggc atactccttg ggccaaggcg
       61 gcgatccgtg agtcgaaacg agtgaagtga tgggccaggg tgcgaactcg gaccgatttc
      121 tgatgaaggc gatgagggag gccggacttg ggcgcccaac ccgcccggtg gttgccctgc
      181 tcgccatgat gatccttgct gcacccatct ggttaatgtc cgaccagctg cgaggctttg
      241 ccctgcagcc gactgacttc gtttacatcc agcaatcacg cgacgctcga gcgttgcggc
      301 agaacctgat ggctcctctc aatacacatg tagtgccttt cttccgactc tggacgtatg
      361 ccctactctc tacggcaggc agcctcgaag cgctaccccc ggttttctcg gcggcgtcat
      421 acttctcgct ggtcgtggca atggttttga tcggcctgct tgtccaacaa gaaacggggc
      481 gtgcggcagt ggccctgtcg gccatggctt gccttgggag ttcgacggtg atgctgccgg
      541 cggtgtgcca ttatgctgca ggccaggcgt tgtgggcggg ggcgggcgcc gtggcgactc
      601 tcctcgccgc gacggggtgg cggacgaagg ggggggcctg gagattcggt ctcgcctggt
      661 tgggggtgat cgcctctcct ttcatctggt cgggcgggct gacggccggg ccggccgttg
      721 ccgcggcact gtgggccgac ggccggccga gatgccgtcg cgcggccgcc cttatggcgg
      781 tcacgacgtg cgtggtcggc gcggcgattg cctgggccag tagagattac atccggcaca
      841 cgccaatggt ctgggaacgc cacagcgagc tctggccgag gcccgtccag gcagtcttgc
      901 actcctccca ggcaattgcc gaggtcctga tcttccgcaa cctgggcgtc gacgcggaaa
      961 catcgccgtt ccaggccacg gtgatcgtcg cctgcctggc ggggctctgg tacgccagcc
     1021 gcggaaggtt ttctcgagcg acgccgctgg aggcggccgg agcggccgtc gttaccgtca
     1081 gcgtgttgat gacttacttc ttcaggggga acctgccgta ccgcagtgtg cggcccgtcc
     1141 tctggtatta cctcataccg caggtcgggg cggtgctcct ggcggccggc tggtgggtcg
     1201 gaatcgggcg cgtggcacgc tgcaccggtg aagaactcac ctggggaggg ctgttggccg
     1261 tcctgacgct cgccttcgtg ctcctcaagg tccaggccgt gcgcgccgag cggatcctga
     1321 tcgaggatgc ggccccactc gccccgtcgg aattcaccaa gttccctctc accaagttga
     1381 agcggttgcg ggcgatctat ttgaatcacg aatcagccaa actccagcgg cggatgctcg
     1441 ccagactgga ccgggtcgag caaattgccc gccggaaggg catcggcaag gcggccatcc
     1501 ggcagacctt tggccgtgtc ttggtgccgg ggctgcccgc ggacctgcaa ccctacgacg
     1561 ccgcagggat gctcagcctt cccgagggcg acccgaacct tagcccgatg ttggtcagct
     1621 cggcgttggc cgactattac tgggaagaag ccgcaccgcg tccgccgtgg ttgctcatta
     1681 cagacccctg gccggtggat gccggggaca ggcgatagac ctgatcggct ggctgatccg
     1741 cccattgccg agccatcatg tcctcaccgt ctcgatcagg gatgaagtgc ctggccctgc
     1801 tggacaatat cggcggattc aatccaccac tgaccatcgc gcttttgcag cacgtaggtc
     1861 cgcaccgatt tccccgtctt ttcgtcggtg agctcatgtg ccagtgcgac gtcgggcgtg
     1921 aggaaccgga cttgttcgat cttggacgac cgagactcga gcataggtcg gatcggcgag
     1981 ccatggagcg tgatcaagtg ctcgcgaatc gcggcacggc ctttcatcca ttgaccaaac
     2041 cgattgaccg catcggcatt ctcgcccatc cgcgccgtga attctttcat ttcgtgcttg
     2101 ttccattcct ccccaagatc gtgcaacaag gcacgaatcg tcttctcgtc accctccttc
     2161 gtcgcatccg gggcccctgg ccggggatcg ccggccgcga tgaacaatcc gaccgagaca
     2221 accgccgcga taaccaatgt gagtgacttc atgctttgct ccgttctgat tgaatcacat
     2281 gcgaaatcaa acctcgacag ttctgagtaa gtatttacta agttattctc gaaaaaaaat
     2341 caatactgag ttgccgaaga ttttttgggc acgaggtaac ggcggatggc cttttctttc
     2401 agtccgatcc cccgcaaggc gaaaaggacg gcctcctcga taagcgtcgc cttggatacg
     2461 ccgtagtcga cgattggcgg gtcggcaagc tgatggagca tcaggattat tgcaaggtga
     2521 tgagtgaacc atccttggat actgaagtgt gaatggctga cattgagatc gtgcgcgacg
     2581 accgcggcct tgatacactc ttcgagcttt gcaaccattc gagaggggat tctctgaata
     2641 aacaggcgag cgaactcgcc gtcttccacg acgctccgaa acatgagtcg cgcgatgttg
     2701 gcaacatccg ctttatcggc ccgtccgcgc ggatcgatca ggctcttata gaattggtgg
     2761 acgatcaaga ccagcgtggc ggtggagggt tccatcgcga ccaattggct gttgacgtcg
     2821 ggttcgtgct ccccgaacaa agccgacagc atggcggtgt aaagagcttc cttggtgggg
     2881 aagtgctgaa acagcaatgc ctcggagacg cccgcggcct cggccagtgc cttggtcgtc
     2941 gtgccgcgga atccgttttc ggcgaagacg cgccgggcgg ccttgagtat cgaatcgcgc
     3001 cgctcttcac tggagagccg ccgtgttttt gccattctca gttccttctg aaacgaatgc
     3061 atagcattcg attactgagg atataccaat caccctcccc agccaagggt caatgacagg
     3121 cctcgatcca gcgtcaatca atctgctgcc gccacctcgc cactatatag aatcggcgat
     3181 tggtccgact ttcggaatgg cgccgccgta gttcgtcgca gcgaggcgtt agctgagctc
     3241 gcggattggg tcccggcggt cgaggaggta gtgggggcgg ttggtggcgt cgtagatcat
     3301 ggtgttctgg acgtcgatgc cgagctggtg atagagggtg gcgaagacgt cctgatagtg
     3361 cacgggccga tcttgcggtt cctcgcccag gcggttggtc gaaccgatga cctggcccat
     3421 gcgcatgccg ccgccggcca ggagggcgca cgatgccttg ggccaatggt ctcgtccggc
     3481 agatttgttg atgcgtgggg tgcgcccgaa ttcgccccag gcgatgacgc tgacgtcctg
     3541 gtccaagcca cgagagtgta aatcttccac gagagcggtg attccctgat cgaacttgtc
     3601 gagttgagga gggagcttgg cgaagttgcc gccgtgtcgg tcccaactgc cgaaggagag
     3661 ggtgacgcac cgggcccctg cttcgaccag gcgtcgcgcc aggaggaact ggtccatcca
     3721 atggggtccg gcgtcttcgc cgaaggcggg gtcggcactg ccgcggccgt agcgatctcg
     3781 gagcttggga tcctcgcgtt cgagatcgag cgcttcggcc agtttattag aggtgaggac
     3841 ctcgaaggcg gcttgcgtga agtcatcgag cccctgggag atggcctgtt gctcggtccg
     3901 gcgacggaag gagtcgtagc tggcgaggag gattcggcgg tcacgcaggc ggtccagggt
     3961 tacgccgtgg agtcgcattt cggtcatttc gggaccgtcg gggagaaacg gggaatgggc
     4021 cattcccagg aagccggggc cgcccgggtt ggaatagggg aggtgcttgg ttttgatcgt
     4081 gagaccgatc gacgggggta ttgccgggtc gatgggtccc ttgagcttgg agatgaccga
     4141 gccgagcgag ggatggccgt cttgattgcc tcgtggaccc ttgggatagc cgcttaagca
     4201 catgtcggag ttgtgctgat cgggactgcc gtagagcgag cgaatgatgg tgaacttgtc
     4261 catcattttg gcgagacgcg gcatgtgctc gcagatttcg atgccaggca cgttggtggg
     4321 aattggccgg aaggagccac ggatctcggc cggggcgttc atcttgagat cgtacatgtc
     4381 ctgatgtgag gggccgccgg acagatagat catgatgacc gctttgtgcg atcgcgtgcg
     4441 cgggcccaat gaatccgtca gtgtctgcgc tttgaggatc tggggcaatg ccaggccccc
     4501 catcgccagg ccgccgatct tcagaaagtg gcgccgggag tggccatcgc aatacgccgc
     4561 ggatttctgg ccaagaatgg aaagcatggc gcggttctcc cgtggacggc tggcccctgt
     4621 cggctacaaa ccggcaggtt ttaccaggac tgcatggagg tcgttcatgt ctaattgcca
     4681 acgggcatct gcggcgacac acgatatcct gtcatggatc tccaattccg tttgtcaggc
     4741 taaaagggaa ttacccccag tgccgggcat gcgaggtcga ccaggctgct acattgtgca
     4801 aggccccacg aacgccgaat ccatcacgtg acgttcgata ttcattgtag gtcgctgttg
     4861 aacgcttgtc aaacaagcgg tcggtgcggg aaaaatgcgc cttgtttgct gatcacggtt
     4921 gaacaataat ttgacaaact ccgtggaaca atcgccgaca ttccccgcag ccgagagcgc
     4981 aatcgatcat gcagctcact gcctcgatac ccgatcgttg tatctgctgc ctggcaatcg
     5041 cactcaccgg gttcatcagc attga
//
LOCUS       DATAQL010001093         8558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2097394, whole genome shotgun sequence.
ACCESSION   DATAQL010001093 DATAQL010000000
VERSION     DATAQL010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097394"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4171)
                     /locus_tag="VHS97_18865"
     CDS             complement(<1..4171)
                     /locus_tag="VHS97_18865"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3450324.1"
                     /translation="MTQPSAERSLIDPLDVVAEEFVRRFREGERPSLSEYAVRYPELA
                     ERIGELFPALVRMEEAGSIVGPLAAMARLAPRVDDQIGEFRILRKIGAGGMGLVYEAV
                     QESLGRHVALKVLTVQRDRIYLERFRREARTAARLHHTNIVPVFAVGETAGIHYYVMQ
                     FIHGQGLDTVLRAVRVLRTPETDAASDSSTAATSVAKSLHDGNFVRAMTEGGEPSVVV
                     TSDASGGDLPIRSELLYYREVARLGAQAAEALAYAHSQGVLHRDVKPSNLLLDTHGTL
                     WITDFGLAKADDSDDLTKTGDLVGTLRYMPPERIRGRADARSDVYSLGATLYEMLTLR
                     PAFPQSDRLELIERIGREAPPPLRRHEPRIPRDLETIVLKAIARDPADRYRTAADLAE
                     DLRRFLNDLPVRARRITWAEELWRWSRRNRTIAGLAASVVLLLAVLAAGASLLAYKTG
                     QSRDQARRAELERTQQLAQSLLSQARAARSSRRRGQRFQGLAAIRSAQLLARESGEPE
                     SFQDKLRTEAIACLALPDISAPVDDSALRPLPANLMAVSPTVYVVLDTAGFVQVTRTQ
                     DQQVLARWQFYPGHPIGRLSHDGSIVAIRSDSGAFRAWNLTTNSAVPLLDRAAGVRAH
                     DIRLDGQELALGLENGTVVLWDIADQATSLLFVPGGPAADLAYSPDGQRLAVVGADTI
                     RVVDCRMSRVVASLKQPSRIASVSWATDNRRMAVGCADLEIILWDVNSSRELHRLPSR
                     SDNGSVAYTPGGELLLSSGWDGMLRVWDPDGRQQRLGFYAAPHFRLSRDGRIWIGAPG
                     RPVAQVELATGQEYQTLERLHLPDLKIATIAAHPGGRILAAAFHRGIALYDMATGQLA
                     ASLPGSCYRLTFDGNGDLIAHGQSGLFHWPIRSTSEANLVVGPPARIEAPEPDGIMAI
                     GCSRDGRVIAGTFQGGPAVLHRDHPDHVTWLGRQMAVRTVAVSPDGVWVAAGSWDTGE
                     GLWIYSAATGRPETRLEARSEVSAVFSPDGRYLVVGSKATSQKIVRVETWNVVAEVAG
                     LAVAFTGDGRLMAVAGEDGPIRLLELETMRELARLEPPDPDRAYWMAFAPDGSRLAVA
                     YSLDKSITVWDLGRIRRQLAEMNLDWQAPPIAEEPVPARPLVIRVVGAARTEPAIEYV
                     LDLAMWGLAALKNPLAAEPHERLGWAFARHGAANKALSEFDRAVKLRPEHAPTYFRRG
                     LTRLEVRTDFDRAAADFGKALELEPGWIGARLNRAKCLARACHWSSALADADAILAAQ
                     PWNADASLIRARSLRNFKRHREAMAELDAAISTYPFRALLYLWRSASRRAVGYDPGSA
                     ADLTRYFQLVPPEYANYDAWRLLTGPAAVRDYEQAELIARCVIERAPRQAHFHNTLGL
                     AFYRLGRFEEAE"
     gene            complement(4168..4821)
                     /locus_tag="VHS97_18870"
     CDS             complement(4168..4821)
                     /locus_tag="VHS97_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450325.1"
                     /translation="MESATNPNSETTLQLLSQAAAGDAVGWGALLRRYHDRLRRMIAV
                     RLDPRLQGRFDPSDVLQETYLDAFGRLQDYLREPRLPFFLWIRFLTGHHLGRIHRDHL
                     GRQMRDPGREVSLYQWAMPEASSAALAAQLLGRESRPSELAVRAERILRLQEALGRMD
                     PIDREVLSMRHFEHLTRAETAHVLKLSEAAAAKRYLRALERLREMLAMLPGGLEEFR"
     gene            5153..5226
                     /locus_tag="VHS97_18875"
     tRNA            5153..5226
                     /locus_tag="VHS97_18875"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5187..5189,aa:Asp,seq:gtc)
     gene            complement(5480..5989)
                     /locus_tag="VHS97_18880"
     CDS             complement(5480..5989)
                     /locus_tag="VHS97_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450326.1"
                     /translation="MSLGHLNYGSWFFRVISWDAILPFCLLIVPMLIELGFPNQRGVI
                     EVVAVTLPIAAFLLRIRVGWRHITTNRCSDFVQRFQFFVFCLGILPLVLIDCFMILSH
                     LMPQGALFATRDDVIVLAIVFAIYLTLMTVAMYPGRALSSPADWDVLIESDELHPRRI
                     QQENVFPEC"
     gene            complement(5993..6256)
                     /locus_tag="VHS97_18885"
     CDS             complement(5993..6256)
                     /locus_tag="VHS97_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450327.1"
                     /translation="MSSPTTDPTLSVIIQLPSDLARTIDVLAKERGQDRDEFMVGFLQ
                     EKLSRSARSFEEVLAPIADNFRVSGTTEEDLDALIEQELLRSR"
     gene            6470..7600
                     /gene="speY"
                     /locus_tag="VHS97_18890"
     CDS             6470..7600
                     /gene="speY"
                     /locus_tag="VHS97_18890"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX3450328.1"
                     /translation="MDFLESISQHRIAPPAVSGNEKVADLIDQAFSSYNAGRLREVCK
                     VFTHKFLEPDCTVGLTLSGALTPAGLGMSCLIPLIKAGFVDWIVSTGANLYHDTHYAL
                     DLPLHQSRPNLDDLALRRNDVIRIYDIVFDYKTLLDTDAFYRELIRDESFARPMGTAE
                     FHHQVGRYLHGRAQKLNRPSPSLLAAAFEADVPIYTSSPGDSSIGMNLAALSLQGGKL
                     QIDSLRDVNETAAIVYAAKRTGGKSAVLILGGGSPKNFILQTEPQIQEVLGLSESGHD
                     YFLQITDARPDTGGLSGATASEAMTWGKVDPATLPDSVTCYTDSTIALPLLTAYALAR
                     HAPRPLRRLYQQRSVQYETLRAEYQAKQLDAGSEPTVRGGVS"
     gene            7623..>8558
                     /locus_tag="VHS97_18895"
     CDS             7623..>8558
                     /locus_tag="VHS97_18895"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX3450329.1"
                     /translation="MARSSSTPVVALDPDQPDPAVLDLAAGIVKQGGLVAFATETVYG
                     LGAVATNSAAVARIFEAKGRPTRNPVIVHVTGVPQARQCVDDWPKAAETLAARFWPGP
                     LTLVLKRSAIIPNVVTAGLDTVAVRAPLGKVALGLIERTGEPIAAPSANRSNRLSPTH
                     ALHVLADLDGRIDLVLDSGPTAVGLESTVIDLTMPVPRVLRPGPIAASKLASALGGAR
                     LIEHVPSDSIDRPSSPGQMPVHYAPSTSSFRADSLAELETIATRHDDIAVVIVGEQST
                     PSLPQFVRQVVLETPEIASRLLYETLHRYDLLGLSA"
BASE COUNT         1686 a         2679 c         2596 g         1597 t
ORIGIN      
        1 cctcggcttc ttcaaaacga ccgaggcgat aaaatgcgag cccgagcgtg ttgtggaagt
       61 gggcctggcg tggcgctcgt tcgataacgc agcgagcgat caactcggct tgctcgtaat
      121 ctcggacagc cgcgggacca gtcaagagcc gccaggcatc gtagttggcg tactcgggcg
      181 gaacgagctg gaaataacgc gtaaggtctg ccgcggatcc cggatcgtag ccgacggccc
      241 gacggctggc ggagcgccat agataaagca gagcgcgaaa agggtaggtg gaaatcgccg
      301 cgtcgagttc cgccatggct tcgcgatgtc gcttgaaatt gcggagtgat cgagcgcgaa
      361 tcaaggaggc gtcggcgttc caaggttgag cggcgagtat tgcgtcagca tcggcgagag
      421 ccgaggacca atggcatgcc cgagcaagac atttcgctcg attgagccgg gctccaatcc
      481 agcctggctc aagttcaaga gctttgccga aatccgccgc ggcacggtca aagtccgtcc
      541 gcacttccag gcgtgttagt ccgcgtcgaa agtaggtcgg cgcgtgttcc gggcgcaatt
      601 ttacggcgcg atcgaattcg gacagcgctt tattggcggc gccgtgccgc gcaaacgccc
      661 agcccagtcg ttcgtgtggc tcggctgcga gcgggttttt gagcgcagcc aggccccaca
      721 tggccaagtc tagaacatac tcgatcgcgg gctccgttcg cgcggcgcca accacacgaa
      781 tgacgagagg ccgcgcggga actggctctt ccgcgatggg cggcgcctgc cagtcgaggt
      841 tcatttccgc caattgtcgg cgaatccggc cgaggtccca cacggtgatg ctcttgtcca
      901 gcgaataggc gactgcaagc cgagaaccat caggtgcgaa tgccatccag taggctcggt
      961 ccggatccgg cggctcgagc cttgccagct ctcgcatggt ttccagttcc aagagccgga
     1021 tcggaccgtc ctcaccggcg accgccatca agcggccatc cccggtgaat gcgaccgcca
     1081 agccggccac ctcggccacg acgttccagg tttcgacacg aacgatcttt tgtgaggtcg
     1141 ctttcgaccc gacgacaaga tagcggccgt ccgggctgaa aactgctgag acctcgctgc
     1201 gggcttccag gcgagtttcg ggccggcccg tggccgccga gtaaatccac aagccctcgc
     1261 ccgtgtccca gctaccggct gccacccaga ccccgtcggg actgactgcc accgtccgaa
     1321 cggccatctg gcggcccagc cacgtcacgt gatcgggatg atcgcggtgc agcaccgccg
     1381 gcccgccttg aaaggttccc gcaatcaccc gcccgtcgcg actgcagcca atcgccataa
     1441 tgccgtcggg ctccggggcc tcgattcgcg ctggtgggcc gacgacgaga ttcgcttcag
     1501 aggtcgagcg gatcggccaa tggaagaggc ccgattgtcc gtgtgcgatc aggtcgccat
     1561 taccatcgaa tgtgaggcga tagcacgagc cgggaaggct cgcggcgagt tgacccgtag
     1621 ccatgtcgta tagagcaatc ccgcgatgaa atgccgccgc caatatgcgg ccgccggggt
     1681 gggccgcgat tgtcgcgatt tttaggtccg gtaaatgcaa ccgctcgagt gtctgatact
     1741 cttgtcccgt agcgagctcg acctgggcaa ctggtcgccc gggtgcgccg atccagatcc
     1801 gtccgtcgcg gctgagccgg aaatggggag cagcataaaa tccgagtcgt tgttggcggc
     1861 cgtcgggatc ccaaactctc aacatgccgt cccagccgct gctgagaagt agttctccac
     1921 ccggagtgta ggcgacggag ccgttgtcac tgcgactcgg cagcctgtgg agctcacggc
     1981 tcgaattcac atcccagagg atgatttcaa ggtcggcgca tcccacggcc attcgccggt
     2041 tgtccgttgc ccaactcacc gatgcgatcc gactgggttg tttcaagctc gctacgactc
     2101 gggacatccg gcaatcgacg actcgaatag tgtcggcgcc aaccacggcg agccgttgtc
     2161 catcgggaga ataagcaaga tccgcggccg ggccgcccgg gacgaagagc aaactggtag
     2221 cttggtcggc tatatcccaa aggacgaccg tgccgttctc aagtcccagc gccagctctt
     2281 ggccgtcgag gcgaatgtcg tgagcgcgga cgccggccgc gcggtcgagt agggggaccg
     2341 cactattcgt ggtaaggttc cacgcgcgaa atgctccaga gtcggagcgt atcgcgacaa
     2401 tcgaaccatc gtggctgagc ctcccgatgg ggtggcccgg gtaaaattgc cagcgcgcca
     2461 gcacttgttg gtcttgtgtt cgggtaactt gaacgaatcc cgccgtatca agcacgacgt
     2521 agacggttgg cgagactgcc atcaaattgg ccggcaacgg ccgcagagcc gagtcatcga
     2581 caggagcgct tatatctggc aacgccaagc aagcgatggc ctcggttcgc agcttgtctt
     2641 ggaacgattc gggctcgccg gactctcgtg ccaacaattg agcggaccgg atggcggcta
     2701 ggccctgaaa tctctggccg cgccgacggc tggatctggc ggccctggcc tggctgagga
     2761 gggactgcgc gagctgctgg gtacgctcga gctcggcccg ccgcgcctga tcacgcgact
     2821 gtcccgtctt gtaggcgagc agcgacgccc ccgcggcaag gacagccagg agcaacacaa
     2881 ccgatgcagc aagtccggcg atcgttcggt tacgtcgcga ccagcgccat agctcctcgg
     2941 cccacgtgat ccgccgagcc cgcactggca aatcgttcaa gaaccggcgg aggtcctccg
     3001 ccaggtcggc cgcggtgcgg tagcgatcgg cggggtcgcg cgcgatcgcc tttaaaacga
     3061 tggtttcgag atcgcgggga attcgcggtt cgtggcggcg caaggggggc ggcgcttcgc
     3121 ggccgattcg ttcgatcagt tcgagccggt cgctctgagg gaacgccggt cgaagtgtga
     3181 gcatctcgta aagagtcgcc cccagcgaat agacgtcgga ccgtgcgtcg gcgcggcctc
     3241 ggatccgctc cggcggcatg taacgcaagg tcccaaccag gtcgccggtt ttggtgagat
     3301 cgtcggagtc gtcggccttg gcgagaccga aatccgtgat ccagagggtt ccgtgtgtat
     3361 ccagcagcag gtttgagggc ttcacgtcgc gatggagcac gccctggcta tgagcatagg
     3421 caagtgcctc ggctgcctgt gctcccaatc gcgccacttc gcgatagtaa agcagctccg
     3481 agcgaatggg aagatcccct ccgctggcgt cgctagtgac cacgaccgac ggttcgccgc
     3541 cctcggtcat ggcacgaacg aaattgccat cgtgcaggct cttggcaact gatgttgctg
     3601 ccgtactcga gtccgaggca gcgtcggttt ccggggttcg cagtacgcgc accgcccgaa
     3661 gcaccgtgtc gagtccctgt ccgtgaataa actgcataac atagtaatga atgcccgccg
     3721 tttcaccgac cgcaaacacc ggcacgatgt tggtgtggtg caagcgcgca gctgtcctgg
     3781 cttctcgacg aaaccgctcg aggtagattc gatcgcgttg aacggttagc accttgagtg
     3841 cgacgtgccg gcctaacgat tcttgtactg cctcgtaaac cagccccatg cctcctgcgc
     3901 cgatcttacg caagatgcga aactcgccga tctggtcgtc cacgcgcggc gcgagtcgcg
     3961 ccatagccgc cagcggtccg acgatggacc cagcctcttc catgcggacc aaggcgggaa
     4021 acaactcgcc gatgcgctca gccagctctg gatagcgaac ggcatattcc gagagcgacg
     4081 gtcgctcgcc ttcacggaag cggcgcacaa attcctcggc aacgacatca agggggtcga
     4141 tcaacgagcg ttcggcggaa ggctgcgtca tcgaaactcc tccaagccgc ccggcagcat
     4201 ggcgagcatc tcacgcaggc gctcgagcgc ccggaggtac cgcttggcgg cggctgcctc
     4261 cgagagcttc aagacgtggg ccgtctcggc gcgcgtcaag tgctcgaaat gtcgcatgga
     4321 caggacctcg cggtcgatcg ggtccatccg tcccagcgct tcctggagtc tgaggatccg
     4381 ttcggcccgc accgccagct cgctgggccg tgattccctg cccagaagct gcgcggccag
     4441 ggccgccgac gaggcctcgg gcatcgccca ttgatagagc gacacctcgc gcccagggtc
     4501 gcgcatctgg cgccccagat ggtctcgatg gattcgcccc agatgatggc cggtcaggaa
     4561 tcgaatccag aggaagaagg ggagtcgcgg ctcgcgcaaa taatcctgca agcggccgaa
     4621 ggcgtccaag taggtttcct ggagcacgtc ggagggatcg aaccgccctt gaagtcttgg
     4681 gtcgagccgc acggcgatca tgcgccgcag ccggtcatga tatcgtcgca aaagtgcacc
     4741 ccagccgaca gcgtcgcccg ctgccgcctg gctcagcagt tgtaaggtcg tttctgagtt
     4801 aggattggtc gcgctctcca tataataatg aatgatccaa gatcaacgcg gacgggacaa
     4861 ggatgatccg cgggttgcga caagggcgat agttgacgtg gcgcgagtaa gtcgagtcgc
     4921 tcgaaagata agcgcctaaa gaccttgctt gtacgaccgt aaactcgttt tgggcgatcc
     4981 gactccgcat ctggtccgac gcagcccttg aagcagccga cagtgcccgc tacaatcttc
     5041 tttcatgggt tgtccgacac ggtgcgaagc cgattctctc ggccgtcctt tcagacaaat
     5101 cttgtgagcc aagatcgagc gggtttaggc gagtggtacc ccgttccgat ttggggatgt
     5161 agctcaattg gttagagcac cggcctgtca cgccggaggt tgcgggttcg agccccgtca
     5221 tcctcgcttt cacatcgacc gcgcaagtcg aacggaggaa ttaggagctt ggcgacgact
     5281 aatccctcgc caattcccgc cgctctaact ccgatcggtt ccacgattgc aacaccaacg
     5341 ctcgacgaat tgtaccggat gaccgcggag ccagatcagc gtgccgtcat caaagacgtt
     5401 gactggtcgt tttgatcgct tcaatgccgc taacgaacgg tcaaaggcct gccggccacg
     5461 gcgcgcttcc tcgcctcact cagcattcgg gaaatacgtt ctcttgctga atccttcgcg
     5521 gatgcaattc gtcactctca atgagaacat cccagtcggc aggggacgat aacgctcgcc
     5581 cgggatacat ggcgacggtc atcaacgtca gatagatggc gaacacaatg gccagcacaa
     5641 tgacgtcgtc acgtgtagcg aacagcgccc cctggggcat cagatgggaa aggatcatga
     5701 agcaatctat gaggaccaat gggaggatgc cgaggcaaaa gacgaaaaac tggaatcgtt
     5761 gaacgaagtc tgaacatcga ttcgtcgtga tgtgacgcca tccaacgcgg atgcggagta
     5821 agaacgccgc gatcggcagc gtcacagcga cgacctcgat gaccccgcgc tgatttggga
     5881 agccgagctc gatcaacatc ggcacaatta acaaacagaa aggcagtatg gcgtcccagg
     5941 agatgaccct gaaaaaccag gagccataat tcagatgccc aagcgacatc ggctatcgac
     6001 tccgcaatag ctcctgctct atcaaggcat ccaggtcctc ttcggtcgtg ccgctcactc
     6061 taaagttgtc ggcaattggc gccagcactt cctcgaacga ccgtgccgag cgactaagct
     6121 tttcctgaag aaagccgacc ataaactcgt cacggtcttg accgcgctct ttggccaaaa
     6181 cgtcgatcgt tcgagccagg tccgatggga gctggataat cacggagagc gtcggatcgg
     6241 ttgtcgggct gctcatggta aggtcgctct tcgccatgtg gccttgatgc tatgacgaca
     6301 atgccacaat aattctacct ggtaatcggg gtcgactatc aaacgtgctg cacgattccg
     6361 tcacaatggg ccgcttacca agctggctcg cccggagcct taccctttca gatttgctca
     6421 agaccatcaa gcggaaggat gccgccggtc gagccgagat ttatcgatca tggattttct
     6481 cgaaagcatc agccagcatc gcatcgcacc gccggcggtc tccggcaacg agaaggtcgc
     6541 cgacttgatc gaccaagcct tctcgagcta caacgccggg cggctgcgtg aggtttgcaa
     6601 ggtcttcacg cacaagtttc tcgagcctga ctgcaccgtc ggcctcacgc tctcaggagc
     6661 attgacgccg gccggattgg gaatgagctg tttgataccg ctaatcaagg ccggctttgt
     6721 cgactggatc gtgtcgacag gcgccaatct gtatcacgat actcactacg ccctcgatct
     6781 cccgctgcac cagagccggc ccaatctcga cgatctggcc ctccgccgca atgacgttat
     6841 ccggatttac gacatcgtct ttgattacaa gacgctgctc gataccgatg cgttttatcg
     6901 agagctgatc cgcgacgagt cgtttgcccg gccaatgggg accgccgagt tccaccatca
     6961 ggtcggacga tatcttcacg gccgtgccca aaaactcaat cgtccgtctc ccagcctgct
     7021 ggccgccgcg tttgaagccg acgtgccgat ttacacctcg agccccggtg acagctcgat
     7081 cggtatgaac ctggctgccc tcagcttgca aggaggcaag ctccagatcg attcgctccg
     7141 cgacgtcaac gaaaccgccg cgatcgttta tgccgccaaa cgcacgggag gcaagtcggc
     7201 cgtcctgatc ctgggagggg gcagccccaa aaacttcatc ctccaaaccg agccgcagat
     7261 ccaggaggtc ctcggtctct ccgagagcgg gcacgattat ttcctccaga ttaccgacgc
     7321 ccgtcccgac accggcgggc tctcgggcgc aaccgccagc gaagcgatga cctggggcaa
     7381 ggtcgacccc gcgacgcttc ccgactcagt gacctgctac acggattcga caatcgcact
     7441 gccgcttttg accgcgtacg cactggcgcg ccacgccccg cgtcccttgc ggcggctcta
     7501 ccagcagcga tccgtccagt atgaaacgct ccgcgcggag taccaggcca agcagctcga
     7561 cgccggttcc gagccgaccg ttcgcggagg cgtttcctga aacggcttag agttgctcgc
     7621 ccgtggcacg ctcctcctca actcctgtcg tcgcattgga ccccgatcag cctgatcccg
     7681 cggtactgga tctggccgcg ggcatcgtaa agcagggggg gctggtcgca tttgcgaccg
     7741 agactgtcta tggtttgggc gcggtcgcca ccaattccgc agccgtcgca cggatcttcg
     7801 aggccaaggg aaggcccaca cgcaatccgg tgatcgtcca cgttaccggt gtgccgcagg
     7861 cgcgccagtg cgttgacgac tggcctaagg cggcggaaac cctggccgcc cgattctggc
     7921 cagggcctct gactctcgtg ctcaagcgat ccgcgatcat acccaatgtc gtgacggccg
     7981 gactcgacac cgtcgcggtg cgcgctccct tgggaaaggt tgctctcggg ttgatcgaac
     8041 gtaccggaga gccaatcgcc gcaccgagcg cgaaccgctc gaaccggctc tcgccgaccc
     8101 acgcgcttca tgtgctggcc gatcttgatg gtcgcatcga cctggtcctt gacagcggcc
     8161 cgactgccgt cggcctggag tcgacagtga tcgacttgac catgcccgtt ccgcgcgttc
     8221 tccggcccgg cccgatcgca gcgagcaagc tggccagtgc gttggggggc gctcggttga
     8281 tcgaacacgt gccgagcgac tcgatcgatc ggccgtccag tccgggacag atgccagtcc
     8341 actatgcgcc ttcgacgtcg tcgtttcgtg ccgattcact ggccgagctg gagacgatcg
     8401 cgactcgcca tgacgacatt gccgttgtca tcgttggaga gcaatccacg ccgtccttgc
     8461 cccagttcgt ccgtcaagtc gttctggaaa cgccggagat cgcctcgcga cttctctatg
     8521 agacgttgca tcggtacgac ttgctcggat tgagcgcg
//
LOCUS       DATAQL010001094         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1636778, whole genome shotgun sequence.
ACCESSION   DATAQL010001094 DATAQL010000000
VERSION     DATAQL010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636778"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VHS97_18900"
     CDS             complement(<1..231)
                     /locus_tag="VHS97_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450330.1"
                     /translation="MRVVFRSSAAICGAVLVIAGCAERPATVSDSAPVATAAGPTKVP
                     ASNVNVVKIAAGTDAPKRAQAALINAKAGDVIE"
     gene            complement(432..1502)
                     /locus_tag="VHS97_18905"
     CDS             complement(432..1502)
                     /locus_tag="VHS97_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450331.1"
                     /translation="MRPILFAVVLVASLVLAASPSFAQNRPRLHDIDGFMTKGVFTEL
                     DSALMTHRLSLEEIEKAHFNGQPVDLQDILAWMAKCEKLKFRVEEPLEVRGTISVVLP
                     PNTLGDEAYTVCYYAFNFNGLGLAGSGDELLLVRPEKHPRLPRTGRPWNPDRILAIRL
                     FRLGYLKPDPILRQYKEKIATAAGHAILEKKSNVLIVTDSAPVLEALGRFIDSETLEA
                     MGMPSTGGLAPGEEPRPPSIGAIASRQNIHFYLMALARQRQISLAPVRKPGVASKYYP
                     EGDLWTDEQGYQALQDEYTRISEYVRLVRETGADELGAIDFNRTLSPREQKSLAIRFG
                     LAGSAPVESKTQKTKKKATKKR"
     gene            complement(1657..2325)
                     /locus_tag="VHS97_18910"
     CDS             complement(1657..2325)
                     /locus_tag="VHS97_18910"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3450332.1"
                     /translation="MFSPLRRGARLPLTVLALALAAWSLTGQAAFAHGGGGHGGGGGH
                     GAGGHGGGHSGGHHSGGYHHGGYGGHHGYYPNYYFGGGYGGYGYGYGYNYPYYNYGYG
                     YTYPNYTYSYPSGYYSAYASTAAPLVSAAPTQGRFLGIDEQPVVDAAGNGMQVVRVYS
                     GSAAEQAGLQAGDVIYSANGYLTQQHGNLTWIIGTVPSNGELRMNVRTARDGAVHVVT
                     ARLP"
     gene            2634..3686
                     /locus_tag="VHS97_18915"
     CDS             2634..3686
                     /locus_tag="VHS97_18915"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase"
                     /protein_id="HEX3450333.1"
                     /translation="MNRPFERELAVAIQCVREAARLCRSVAREISPEVLAKKDKSPVT
                     VADFGSQALICRALAALFPHDPVIAEEDAVELKRPENANILQQVLRHVGAVRCETVAR
                     AGDNIDGELVCRWIDHGGGSDNRQRFWTLDPIDGTKGFLRGEQYAVALALVVDGQVMV
                     AALACPNLSGETSDGHVERRTNSGGSIFHAASGQGAYFVADKSAGDDVPGPMAITVSR
                     RDQPADVRFCESVEAGHSAQGDAASVAARIGIIAPPLRMDSQAKYAVVARGEADLYLR
                     LPTRADYREKIWDHAAGALIVTEAGGVVTDITGRPLEFHHGRELAANRGVIVSNGPLH
                     SRIIEAVQSLGIGAES"
     gene            3756..>4032
                     /locus_tag="VHS97_18920"
     CDS             3756..>4032
                     /locus_tag="VHS97_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450334.1"
                     /translation="MAIRAFWRARRDAARRPRYQLTRSCFLRGMGLVYLSAFASLAVQ
                     LDGLFGSSGILPAAEFLERSGKLLGPGPLRYWRLPTVFWFDASDRALH"
BASE COUNT          771 a         1195 c         1236 g          830 t
ORIGIN      
        1 ctcgatcacg tcgcctgcct tggcattgat cagggccgcc tgagctcgct tgggcgcgtc
       61 ggttcctgcc gcgattttca ccacgttcac attggacgcc ggcactttgg tggggccagc
      121 ggctgtagcg accggggctg aatcagacac ggttgccggc cgttcagcac aaccggcaat
      181 gaccagaacc gcgccgcaaa tggccgcgga cgaacggaaa acgacgcgca cgacaggcct
      241 cctcgcccca gcagtgaatt gattggtccc gcggacaact cccggctgac gtgtccgggc
      301 cggaggcggc caccgattgc tgattctaaa ccgaggaact tcgtcatgac aattcatctt
      361 tgagaatacg atcacggcaa gtttgcgact atgccgacaa cctgccgtgc cgagcggctg
      421 gaacgcggct ttcaccgctt tttcgtggct ttcttcttgg ttttttgggt cttgctttcc
      481 accggcgccg atcccgccag accgaaacga atcgccagcg atttctgctc tctcggagat
      541 aacgtgcgat tgaagtcgat tgcacccaac tcgtctgctc ccgtttccct cacgagccgg
      601 acgtattcac ttattctcgt atattcgtct tgaagagcct gatatccctg ctcgtctgtc
      661 caaagatccc cctcgggata gtatttcgac gcgactcccg gcttgcggac aggagccagc
      721 gaaatctggc gctggcgcgc gagcgccatc aagtagaagt gaatattttg gcgggacgct
      781 atcgccccga tgctgggagg tcgcggctct tcgcctggcg ctagccctcc cgtggacggc
      841 atacccatag cttccaaggt ttcggagtcg atgaaacggc ccagggcctc gagcaccggc
      901 gctgagtccg tgacaatcag cacgttcgat ttcttctcga ggatcgcgtg cccagcggcg
      961 gttgcgattt tctccttgta ttgcctcaag atggggtccg gcttgagata accgagtcgg
     1021 aaaagccgga tcgcaagtat ccgatcggga ttccacggtc ggccggtacg cgggagccga
     1081 gggtgttttt cgggccgcac gagcaagagc tcgtcccctg atccggcaag acccagaccg
     1141 ttgaagttga atgcgtaata gcaaactgtg taggcctcgt cgcccagagt gtttggcggc
     1201 aagacaaccg aaatcgtgcc tctcacctcg aggggctctt cgactcgaaa cttcagcttt
     1261 tcgcacttcg ccatccaggc cagtatgtcc tggaggtcca caggctggcc attgaaatgg
     1321 gctttttcaa tctcttcgag cgagagccgg tgcgtcatca gcgccgaatc gagttcggtg
     1381 aagacgccct tggtcatgaa cccgtcgatg tcgtggagtc ttggccggtt ctgcgcgaaa
     1441 gaggggcttg ccgccaagac cagtgacgca acgagcacga cggcaaacag gatgggccgc
     1501 atcgatcgtc tcgccagggt tgagcgtgaa gtttttcgtc aaccctctca gaataaccga
     1561 gactcgtgcg catatcccgc tattctcgaa aaacagggct agccccgtcg catcatgttt
     1621 cggagccagc tccattgcac caggatcgcg ttcgtatcaa gggagtcgtg cggtcacaac
     1681 atgcacagcg ccgtcacgcg ccgtgcggac gttcatccgg agctcgccgt tggacgggac
     1741 tgtgccgatg atccacgtca ggttgccgtg ctgttgggtc aggtagccgt tggccgaata
     1801 gatcacgtcg ccggcttgaa ggccagcctg ctcggcagcc gaacctgaat agacgcgtac
     1861 gacttgcatg ccgttgccgg cggcgtcaac gacaggttgt tcatcgatcc ccagaaagcg
     1921 accttgagtt ggcgctgcgg aaaccaatgg ggccgcggtc gatgcataag ctgaatagta
     1981 accggacggg tagctgtagg tgtaattggg gtaggtgtag ccgtaaccgt agttatagta
     2041 cggatagttg tatccatatc cgtatccata acctccatag cctccgccga aataatagtt
     2101 ggggtagtac ccgtggtgtc cgccgtagcc gccgtggtgg taacctccgg aatggtgtcc
     2161 gccgctgtgc ccaccgccgt gtccaccggc cccgtgtcca ccgccgcccc catgcccgcc
     2221 accgccatgt gcaaaagccg cttgtccggt caaactccag gctgcgagcg ccaaggcaag
     2281 gaccgtcaat ggcaatctcg cgccgcgtcg caatggagaa aacatcgttt ggctcctatc
     2341 cacgttgcct attggcctta atcgaggaat ggcaggcccc ggccggagac ctcgtcgggc
     2401 gtttccgcct cccgatacca tcgattacga tgtcggcagc gcgaccgggc caaaccggac
     2461 gacgaatttc ttcggttttt cctgggtgca agagtaaagc gggtcattgt cgtaaatagg
     2521 caacgcgttc aaactgcgat cgcgaccaaa cgagtcctct ggacaacctg atcgcgtcgg
     2581 atgtcataat gcgatccgac gggccgtcca ctcgaacttt tccggtaact aacatgaatc
     2641 gacccttcga acgtgaactg gctgtggcca tccagtgtgt ccgggaagca gcgcgactct
     2701 gccgatccgt cgcccgcgag atctcgcccg aggtcctggc caaaaaagac aaaagcccgg
     2761 tcacagtggc cgattttggt agccaggcgt tgatctgtcg cgcgcttgcc gccttatttc
     2821 cgcatgatcc ggtgattgcc gaggaagacg cggtcgagct gaaacgcccg gagaacgcta
     2881 atatccttca acaggtactc cggcacgtcg gcgccgtccg ttgcgaaaca gtcgctcgag
     2941 caggcgataa catcgacggc gagttggtct gtcgctggat cgaccacggc ggcggtagcg
     3001 acaaccgaca acgcttctgg acgctcgatc cgatcgacgg cacgaagggg tttctccggg
     3061 gtgagcagta cgccgtcgcg ctggcgttgg tcgtcgacgg tcaggttatg gtcgccgcac
     3121 tggcgtgtcc aaatctgtct ggagaaacgt ctgacggcca cgtggaacga cgcacaaatt
     3181 cggggggttc gatctttcat gcggcgagcg ggcaaggggc ttatttcgtc gcggacaaaa
     3241 gtgccggcga tgacgtgccc ggtccgatgg cgatcacagt cagtcgccgc gatcagccgg
     3301 cggacgtacg attttgcgag tcggtcgagg ccgggcatag cgcacaaggc gacgcagcct
     3361 ccgttgctgc acggattggg atcattgcac ctcctctgcg gatggacagc caggcaaagt
     3421 atgccgtggt ggcgcgcggc gaggccgacc tttacttgcg gctgccgaca cgagccgact
     3481 atcgcgagaa gatctgggac cacgcggcag gggcgttgat cgtcacggag gccgggggag
     3541 tcgttaccga catcaccggc cgcccgctcg agtttcatca cggccgcgaa ctcgcagcca
     3601 accgcggagt catcgtgagc aatgggccac ttcattcgcg gatcatcgaa gcggtacagt
     3661 cgctgggaat cggggcggag tcgtagtggc acgggcatct tgcccgtggg cgttctgaac
     3721 atgggctaga agcccatgcc accaatgagg cgaacgtggc gatacgggcg ttttggcgtg
     3781 cgcggcgcga tgcggcaagg cggccgcgat atcaactgac gcggtcgtgc tttctgcgcg
     3841 ggatggggct tgtgtatctc tcggcgtttg cctcgctggc ggtgcagctc gatgggctct
     3901 tcggctcgag cgggatcttg ccggcggcgg agtttttgga acggtccggt aagctactcg
     3961 gtcctggtcc attgcgttac tggcggctgc cgaccgtttt ctggttcgac gcctcggaca
     4021 gagccctcca ca
//
LOCUS       DATAQL010001095         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4691444, whole genome shotgun sequence.
ACCESSION   DATAQL010001095 DATAQL010000000
VERSION     DATAQL010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4691444"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VHS97_18925"
     CDS             complement(<1..1146)
                     /locus_tag="VHS97_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450335.1"
                     /translation="MSKSHLSRRQFMNQSIAAAGIGAGFAIGGTKSSGRVVGANDTIR
                     LAVAGLNGRGGSHISEFVGMPGVEVTYLVDPDTRTYKRRLDQIKAKGGRAPATVQDIR
                     RVLEDNSVDAITIATPNHWHALMTIWGCQAGKDVYVEKPCSHNVHEGRIAVEAARRHN
                     RIVQHGTQNRSSRDWALAAAAIQSGKLGKLLVSRALCYKSRDSIGVKPTTTPPEQVDF
                     DIWLGPAPKQPYHANLVHYNWHWFWDFGNGDIGNQGVHEMDKARWLIPGASSNGHAAS
                     ATFPKSVLSVGGRFGYKDQGETANTQISVMDYGDTQLIFEVRGLKTNGFHGSKVGNIA
                     HLEEGMLVDGKSFYPKGQTKAVPLGSLVKLEVELGPGKGHFANFIAAM"
     gene            complement(1426..>1990)
                     /locus_tag="VHS97_18930"
     CDS             complement(1426..>1990)
                     /locus_tag="VHS97_18930"
                     /inference="COORDINATES: protein motif:HMM:NF025862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HEX3450336.1"
                     /translation="EHELTVAFTRMLAGPLDFHQGSFRGVPLEKFQQRNVAPVVIGTP
                     CRMLASYVVLQNHLPMVADYPSAYRGHPALPVLSAIPTTWDDTRCVAGKVGEFIIMAR
                     QHGAEWWLGAMGGREPREVEIPLGFLGPGRFRAEVYRDDLNAATRLARRTEDVAAANS
                     LRAPLAPAGGLLIRLSPMNDALKAKGQ"
BASE COUNT          379 a          625 c          589 g          397 t
ORIGIN      
        1 catggccgcg atgaaattcg cgaaatggcc ctttcccgga cctagttcga cttcgagctt
       61 taccagactg cccagtggaa ccgccttggt ttgtcccttg gggtagaacg acttgccatc
      121 gacaagcatg ccctcttcga ggtgagcgat gttgccgacc tttgacccat ggaaaccatt
      181 ggtcttcagg ccgcggactt cgaagatgag ctgagtatcg ccgtaatcca tcacgctgat
      241 ctgggtattg gccgtctcgc cctggtcttt atagccgaac cggcccccca cgctgagaac
      301 cgacttcggg aaagtcgcgc tggcggcatg tccattcgag ctcgcgccgg ggatcaacca
      361 gcgggcttta tccatctcgt ggacaccctg gttaccgatg tcgccgttac cgaagtccca
      421 aaaccaatgc cagttgtaat ggaccaaatt cgcgtggtac ggctgcttgg gcgccgggcc
      481 cagccagata tcgaaatcga cctgctcggg aggtgtcgtg gtcggtttga ccccaatcga
      541 gtcccgcgac ttgtaacaaa gggcgcgaga caccaagagc ttgccaagct tgcccgattg
      601 aatcgccgcg gcggccagtg cccagtcacg gctggaacgg ttctgggtac cgtgttgaac
      661 gatccggttg tgtcgccgcg cagcctcgac ggcgatccgg ccttcatgca cgttgtggct
      721 gcacggcttt tcaacgtaca cgtccttgcc agcctggcag ccccagatcg tcatcaaggc
      781 gtgccagtga ttgggggtgg caatcgtgat cgcgtcgacc gagttatcct cgagaactcg
      841 gcggatatcc tggacggttg ccggcgcacg gcctcccttg gccttgatct gatcaagccg
      901 tctcttgtac gtccgtgtgt cgggatcgac gagatacgtg acttcgacgc cgggcattcc
      961 cacaaactcg ctgatgtgcg acccacctcg tccattgagc ccggccacgg cgagacggat
     1021 cgtgtcgttg gccccgacga ctcgccccga cgattttgta cctccgattg caaaccccgc
     1081 cccgatcccg gcggcggcaa tcgattgatt catgaactgg cgacgactca ggtgtgattt
     1141 tgacatcgct tttgccctct caatgaaccg cctgttgtcc cgatctcagg ccaaatcccc
     1201 aacgaagatc aaacacggcg ctattatgcg cgagtcgacg ctcgattccc agcgtttggc
     1261 gtacaaaaga agctcgcctg tcgaaatagg cgcaagactc tcttcatgtc atcagcaacg
     1321 tccttcaagc atggctcgca gatgatggtc aggtgcgttc ttcgcaccgg cacaaccttc
     1381 gggttcgggg caatcatcga ccacaccgac tctatgacga aagcgtcact gtcccttcgc
     1441 cttcagggcg tcgttcatcg gcgaaagtcg gatcagcagg ccacctgccg gagcgagcgg
     1501 cgcgcggagg gaattcgcgg cggcgacgtc ctccgtgcgt cgggcgaggc gggtcgcggc
     1561 attcaggtcg tcccgataaa cctccgctcg gaaccggcca ggaccgagga agcccagcgg
     1621 gatctcaacc tcgcgaggtt ctcggccgcc catcgcgccc aaccaccact cggccccgtg
     1681 ctggcgggcc atgatgatga actctccgac cttgccggcc acgcagcggg tgtcgtccca
     1741 ggtcgtgggg atcgccgaca ggaccggtag cgccgggtgg ccgcgatagg cggacgggta
     1801 gtcggccacc atcggcaggt gattctggag aacgacataa gacgcgagca tccggcaggg
     1861 tgtccctatg accaccggag ccacgttgcg ctgctggaat ttctctaggg gtacgccacg
     1921 gaacgaacct tggtggaaat cgagcgggcc ggccaacatc cgagtgaatg ccacagtcag
     1981 ctcgtgctcg
//
LOCUS       DATAQL010001096         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5303839, whole genome shotgun sequence.
ACCESSION   DATAQL010001096 DATAQL010000000
VERSION     DATAQL010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5303839"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VHS97_18935"
     CDS             complement(<1..563)
                     /locus_tag="VHS97_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450337.1"
                     /translation="MLYGSLASLLAAFVSLLAAEPPGAEVSWIGRDATRSRITLVDVD
                     GSSPKVVLDSPHRYAAPEWTPDGAELIVNGGGKLWRLSASGGAPKAIPTGMANWIDIN
                     HAGSPDGKSLAFTAGPIWKVPVQGGEPARVTTASGNYLHAWSPDSKRLAFSSNRGNGL
                     DLFSIGTDGLAERRLTTDFHADDAPQYS"
     gene            518..838
                     /locus_tag="VHS97_18940"
     CDS             518..838
                     /locus_tag="VHS97_18940"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450338.1"
                     /translation="MRQRQRAATRGFHITWAERSSGSQGATLRLKADAGRDHFPEAYT
                     AVLAGGGIRAGQVIGRTTVGGVAVEDRPVTVPELLATVCRCLGIDAAKQNASNTGRPI
                     SIVD"
     gene            976..>1555
                     /locus_tag="VHS97_18945"
     CDS             976..>1555
                     /locus_tag="VHS97_18945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3450339.1"
                     /translation="MIVRRRDGAIIRQLQTLYNVGTIGDLTDGQLLERFATDVDEVAE
                     LAFSALVERHEAMVWRVCLAIVRDEHEAEDAFQATVLVLVRKARSLWVRDSLGPWLHQ
                     VACRTASYLRTMGIPRRKLEQRCAQREAARYVEVGTPRDLDLDSAIHEEVNRLPEKNR
                     APVVLCDLEGRTHQEAARFLGWPIGTVKSRQSQ"
BASE COUNT          286 a          472 c          537 g          260 t
ORIGIN      
        1 gaatattggg gggcatcgtc ggcatgaaaa tcggtcgtga gccggcgctc ggcaaggccg
       61 tcggtgccga tcgaaaagag gtcaagcccg ttcccgcggt tggacgagaa cgcgagcctc
      121 ttgctgtcgg gcgaccaggc gtggaggtag tttcccgagg cggtcgtcac gcgcgccggc
      181 tcgccgcctt ggacagggac cttccagatc ggcccggcag tgaaggccag cgacttgccg
      241 tcgggggaac cggcgtgatt gatgtcgatc cagttcgcca tgccagtcgg gatcgccttg
      301 ggcgcgcccc ccgaggcgga aaggcgccag agcttgccgc cgccgttgac gattagttcg
      361 gcgccgtcgg gggtccactc aggcgccgcg tagcgatgag gcgagtcgag gacaactttc
      421 ggcgaggagc cgtcgacgtc gacgagcgtg attcgacttc gggtcgcatc cctgccgatc
      481 catgatacct ccgcgccagg aggctcggcg gcaaggagtg agacaaaggc agcgagcagc
      541 gacgcgaggc ttccatataa catgggcgga acgctccagc ggttcgcaag gggcaacgct
      601 tcgtctcaag gccgacgccg gccgcgacca tttccccgag gcttacacgg cggtcctggc
      661 cggcggcggc atccgggccg gccaggtgat cggccggacg actgtcggcg gcgttgcggt
      721 cgaggatcga ccggtaacgg tcccggagct cctcgcgacc gtctgccgct gtctgggcat
      781 cgacgcggcg aagcagaacg catcgaacac aggccggccg atctcgatcg tggattaaga
      841 ggcaaggccg atcgttgaac tgctcggtaa tcccgtggat tctcagaacc attcaagctc
      901 ctcgtgacat tatagtgaaa tgaacaatac cgggtcagcc ggatcacgac gctatggtgt
      961 gcgagggcag acaccgtgat agtgagacga cgggacgggg ccattattcg tcagctccaa
     1021 accctctaca atgtcgggac catcggggat ctgaccgacg gccagctcct ggaacgattc
     1081 gccaccgacg tcgacgaagt ggccgagctg gccttttcgg ccttggtcga gcggcacgag
     1141 gcaatggtct ggcgcgtctg cctggcgatc gtacgcgacg agcatgaggc ggaggatgcc
     1201 tttcaggcca ccgtcctggt cctggtccgc aaggcaagat cactttgggt acgggactcg
     1261 ctgggtccct ggctccatca ggtcgcctgc cgcacggcgt cttatctcag gacgatgggc
     1321 atcccacgac gtaagctcga gcagcgttgc gcacagcgag aagcggcccg ctacgtcgag
     1381 gtcgggacac cgcgcgatct tgatcttgat tccgcgatcc acgaggaggt gaaccggctg
     1441 cccgagaaaa atcgcgctcc ggtcgtgctc tgcgacctgg agggccgtac gcaccaggag
     1501 gcggcgcggt tcctcggctg gccgataggg acggtcaaga gccggcagtc gcagg
//
LOCUS       DATAQL010001097         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4691543, whole genome shotgun sequence.
ACCESSION   DATAQL010001097 DATAQL010000000
VERSION     DATAQL010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4691543"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /gene="sixA"
                     /locus_tag="VHS97_18950"
     CDS             complement(<1..503)
                     /gene="sixA"
                     /locus_tag="VHS97_18950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX3450340.1"
                     /translation="MTHLYIVRHGIAALPGTPGIPDDERLLTSKGEKRVRQIARGLAR
                     LDLKLDRIVTSPLPRARATAEIIADELDSRKLLEISNVLQTGSSAATVERWLRERTED
                     RLMIVGHNPTMSDLVSLLVLGSIQPTICDLKKGGIVALTRAAAYAELYELHCIAPPRL
                     IRGLMDG"
     gene            complement(500..1399)
                     /locus_tag="VHS97_18955"
     CDS             complement(500..1399)
                     /locus_tag="VHS97_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX3450341.1"
                     /translation="MTEGCIIIMESATAPRILMCPPDHFKIEYEINPWMNIRVGSEAV
                     LARRQWTALYQSLVDLGATVDLIEPVAGLPDLVFTANAGLVFHNIFVSSRFRYGVRQG
                     EAPYYESWALSRDFQIVELPQGFNFEGAGDALFCGETLIAGYRFRSDVRSHHWIGERL
                     GVEVLPLELIDPRFYHLDTCFCPLAPGIALYYPGAFDEYGRSVLRDRIPHLIEVAVDE
                     AISFSCNAVVVGKTVILNEGAPKLAQDLAVRGFGVCPLEFSEFIKSGGSAKCLTIRLD
                     GEEAATWKQSPRTLSVGSPETSR"
     gene            complement(1481..2749)
                     /locus_tag="VHS97_18960"
     CDS             complement(1481..2749)
                     /locus_tag="VHS97_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX3450342.1"
                     /translation="MTEKAIRQTPGAVLTQPPGIVEEIEVRGHIVDSLLLPKILDRIL
                     LMGGTFEIRECTIGVHRTDPSYARIAVQADTHEKLAEILGDLVEHGASPTHSEDANIV
                     AADIAGAFPEGFYSTTNQTTQVRVNKRWITVDNQEMDCGIVVEKGTGHARCIPMYRVE
                     IGMPIVVGHTGVRVVPVERSREVSPFGFMGSTVSSEKPKSISVRSVVDAIRRTRADRK
                     KVLLVGGPAIVHTGSAGHVARLIKEGWVQVLFAGNALATHDIEQALYGTSLGVSLTHG
                     EPLEHGHEHHLRAINTIRRLGGIARAVEVGVLNAGIMYECVKAGVDVVLAGSIRDDGP
                     LPEVISDTLVAQDRMRQAIDGVGFALLIATALHSIATGNLLPALVKVVCVDINPATVT
                     KLSDRGTFQTIGLVTDVEPFLRSLATELSS"
     gene            complement(2785..>2977)
                     /locus_tag="VHS97_18965"
     CDS             complement(2785..>2977)
                     /locus_tag="VHS97_18965"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX3450343.1"
                     /translation="ADAPRGDNVVLDFNKATNLPCAYIPYATCPLAPSQNRFSLAIEA
                     GELMYEPQRVDGSPGEASR"
BASE COUNT          571 a          956 c          872 g          578 t
ORIGIN      
        1 tccccatcca tcagcccccg aatcagccgc ggcggcgcaa tacagtgcag ttcgtacagc
       61 tcggcatagg ccgcggcccg cgtcagggcg acaatgccac cttttttgag gtcgcatatg
      121 gtcggctgga tcgatcccag gacaagaagc gacaccaggt cgctcatggt cgggttatga
      181 ccgacgatca tcagccggtc ttccgtgcgc tcacgcaacc agcgctcgac cgttgcggcc
      241 gacgagccgg tctggagtac atttgagatc tccagaagct ttcgagaatc aagctcgtcg
      301 gcaataatct cggccgtcgc gcgggcccgg ggcagggggc tggtgacgat cctatcgagc
      361 ttcaggtcaa gcctggccag tccgcgcgcg atctgccgca ctcgcttttc gcccttggat
      421 gtcagaagcc gctcgtcgtc tgggatgccc ggcgttcccg gtagggcggc gatcccatga
      481 cgcacaatgt agagatgagt catcggctag tctctgggct cccgaccgat agagtacgcg
      541 gactttgttt ccaggtcgcc gcttcttcgc catcgagccg tatcgtcagg catttggcgc
      601 tcccccccga tttgatgaat tccgagaatt cgagcgggca aacaccaaag cccctcacgg
      661 ccaggtcctg ggcgagcttg ggtgcccctt cgttcaagat cacggtcttg ccgacgacga
      721 ccgcattgca actaaaggag attgcctcgt caaccgcgac ctcgatcaga tgaggaatcc
      781 ggtcgcgcag caccgaccgg ccatactcat caaacgctcc tggataatag agcgcgatgc
      841 cgggtgccag cgggcagaag catgtatcga ggtggtaaaa gcgcggatcg atcagttcga
      901 gcggcagcac ttccacgccc agccgctcgc cgatccagtg gtggctccgc acgtcgctac
      961 gaaatcggta gcccgcgatc agcgtttcac cacaaaagag cgcatcgcct gccccttcaa
     1021 agttgaaacc ctgcggcagc tcgacgattt gaaaatcccg gctaagtgcc cagctttcgt
     1081 aatagggcgc ttctccctgc cggactccgt accggaatcg ggagctgacg aaaatgttgt
     1141 ggaagacgag tcctgcattg gcggtaaaga ccaggtcggg aagccccgca acaggctcaa
     1201 tcagatccac ggtcgccccc agatccacga gcgattggta cagtgcggtc cattggcggc
     1261 gggctagcac cgcttcgctt ccgacccgga tattcatcca aggattgatc tcatactcaa
     1321 tcttgaagtg atccggcgga cacatcaaga tccggggcgc cgtcgcggac tccatgatga
     1381 tgatacagcc ctccgtcata cacacgagcc ggaccggccg gaaacagggt cacactattc
     1441 aagcagacgg caccgatcac gctggcagca acgtccccag ctacgatgaa agttcggtcg
     1501 ccagcgatcg caaaaacggc tcgacgtcgg ttacaagacc gatggtctgg aatgtcccgc
     1561 ggtcggagag ctttgtcaca gtcgccggat tgatatcgac gcagaccacc ttgaccaagg
     1621 ccggcaagag gttgcccgtt gcgatcgagt ggagcgccgt cgcaatcaag agcgcaaacc
     1681 cgacgccgtc tatcgcctgg cgcatccggt cctgagcgac gagcgtatcg ctgatcacct
     1741 cgggcagcgg accgtcgtcg cggatcgatc cagccagtac cacgtcgacc ccggccttga
     1801 cgcactcgta catgatccca gcgttgagca cccccacctc caccgcccgg gcaatgccac
     1861 ccaaccggcg gatggtgttg attgcgcgca agtgatgttc atgcccgtgt tcgagcggtt
     1921 ccccgtgcgt cagcgaaacg cccaggctgg tgccgtagag ggcctgttcg atgtcgtggg
     1981 tcgccagggc gttgccggcg aagagtacct ggacccagcc ctccttgatc agccgcgcga
     2041 cgtggccggc cgaccctgtg tgcacgatcg ccggcccccc cacgagcaat acctttttcc
     2101 ggtcggctcg ggtgcgccgg atcgcgtcga ccacgcttct cacactaatc gacttgggct
     2161 tctcgctcga gacagtcgaa cccatgaatc cgaacgggct gacttcacgc gaccgctcga
     2221 ccggcactac gcgtacccct gtgtggccca cgacgatcgg catgccgatt tccaccctgt
     2281 acatcggaat gcaccgcgca tgcccagtcc ccttttcgac aacgatcccg cagtccattt
     2341 cctggttgtc gacggtgatc cagcgcttgt tcaccctgac ctgggtggtc tgattggtcg
     2401 tgctgtaaaa cccttcggga aacgcgcctg cgatatcagc cgcgacgatg ttggcgtctt
     2461 cggaatgcgt cggcgatgcg ccgtgctcga ccagatctcc caggatttcc gcgagtttct
     2521 cgtgagtatc ggcctggact gcgatccgcg catagctcgg gtcggtgcga tgcacgccga
     2581 tcgtgcactc gcggatctca aacgtcccgc ccatcaacaa gatgcgatcc aggatcttgg
     2641 ggagcaagag cgagtctacg atgtggccgc gcacctcgat ttcttcgacg atgcccggtg
     2701 gttgggtcaa gacggccccg ggagtctggc ggattgcttt ttcggtcatc ggcgcggctc
     2761 cttgccatcg actcttttcg tggactaccg gctcgcctct cctggcgatc cgtcaacgcg
     2821 ctgcggctca tacatcagct cgccggcttc gatcgccagg ctgaaccggt tctgggacgg
     2881 cgcgagcgga catgttgcgt aaggaatgta cgcgcaaggg agattggtcg ccttgttgaa
     2941 gtcgagcacg acattatctc cccggggcgc gtccgcg
//
LOCUS       DATAQL010001098         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_571795, whole genome shotgun sequence.
ACCESSION   DATAQL010001098 DATAQL010000000
VERSION     DATAQL010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571795"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..551)
                     /locus_tag="VHS97_18970"
     CDS             complement(411..551)
                     /locus_tag="VHS97_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450344.1"
                     /translation="MVAAQTAKMITIIDPKTPNLDRTRCGTDLVLDAYSIGVLVRTVN
                     PA"
     gene            565..1839
                     /locus_tag="VHS97_18975"
     CDS             565..1839
                     /locus_tag="VHS97_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450345.1"
                     /translation="MFYLSWRWWRALAEVRRGLEALGSRERARPVLIAAQGPVGRLVR
                     AFNMAANEIQAQTAGLNQDRQQLLVVLGAMTEAVIAVDPRRRLLFANASANRLFGLDW
                     ASVGRLVPELIRSPHVQDAVEETLRLASPAAFEGEVTLPGRDSGQRANNRLLSVRGNP
                     LPGRPSSGAVLVFHDVTELRRLERMRQDFVANASHELKTPLSSIKAYTETLLDWALYD
                     ESVNARFLKRIEEQVERLNQLILDLLSLARIESGQEIFEHGPLYLVPVLESCVESHRD
                     RAQTKNLNLTFDPGRLDEETMVLADEEAIRQIVDNLVDNAIKYTTEGGSVSITCHSDQ
                     NSASIEVADTGIGIPRADLPRIFERFYRVDKARSRELGGTGLGLSIVKHLTQSIGGQI
                     DVTSRLGSGSTFIVHLPRCLVADMRKQGAERT"
     gene            complement(2250..2660)
                     /locus_tag="VHS97_18980"
     CDS             complement(2250..2660)
                     /locus_tag="VHS97_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450346.1"
                     /translation="MNEVLEVPVEEAKQESTDVAENAEFIAVPQETEVAEQVATVQDE
                     VVPTALAEQVTPVQDEVIPTEVPEQVTLAQDEVVSTEAAEQVTPVQDEVVPTAFAEQV
                     APVQDEVIPTEVEEQVTPVYEEVAQVEESIAVNE"
     gene            complement(2975..>3200)
                     /locus_tag="VHS97_18985"
     CDS             complement(2975..>3200)
                     /locus_tag="VHS97_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450347.1"
                     /translation="SLADIGTFGHASRAGPLRRRIARNDADDTGLIAISPSGPTDWCA
                     PVFAQEVVLGVGLNLSSRYLCTPRTGMVQL"
BASE COUNT          602 a         1014 c          960 g          624 t
ORIGIN      
        1 ctatcatcgt gccgattgcg cttacgtgcc ggcgatgtcg gagcggaaca ggatcccgct
       61 tcaaagcccc gaggacgcaa gagcccacgg gctcgagcca tgcgagaagt gccgcccacc
      121 cgtcgccgaa tatgaccggg cagccacgtc aggccagccg ggttgatctg gcgggtctgg
      181 ctgacaatct tgaccgccgc ggggtcgaac cggtagggtg gtaccgaacg atccgcagga
      241 tcgagaggcc caccgcgatc cacggcagaa tggcccaccg cgactcacgg cagaatggcc
      301 caccgcgacc cacggcagaa tggcccaccg cgacccacgg cagaatggcc caccgcgacc
      361 cacggcagat cggtgggcct cgccgcgatt tctcgcgact cggcaccacc ctacgccggg
      421 ttgaccgtgc gcacgagcac accgatactg taggcgtcca gaaccagatc ggtgccgcag
      481 cgcgtacgat cgaggttcgg cgtcttgggg tcaatgatgg tgatcatctt tgctgtctgc
      541 gcggcgacca tagcggcggc agccatgttt tacctgagct ggcgctggtg gcgtgccctg
      601 gccgaagtga ggaggggcct ggaggccctg gggtcgcggg aaagagcacg cccggtattg
      661 atcgcggcgc aggggcctgt gggacggctg gttcgcgcat tcaacatggc cgccaacgaa
      721 atccaggcac agacagccgg gttgaatcag gataggcagc agcttcttgt ggttttgggc
      781 gccatgaccg aagccgtcat cgccgttgat ccccgccgcc gtcttctctt tgccaatgcc
      841 agcgccaatc ggctttttgg tctcgactgg gcctccgtcg gccgtctcgt ccccgagctg
      901 atacgcagtc cgcacgtcca ggacgctgtc gaggagaccc tgcgactggc gagccccgcc
      961 gcattcgaag gagaggttac cctgccgggc cgcgactcgg gccagcgcgc caacaaccgg
     1021 ttgctctcgg tgcgcggcaa tcctctgccc gggcgtcctt cctccggcgc ggttctggtt
     1081 ttccatgacg tgaccgagtt acgccggctc gagcgcatgc ggcaagactt cgtggccaac
     1141 gcgtcgcacg agctcaagac gcccttgtct tcaatcaaag cctacaccga gactttgctc
     1201 gactgggccc tctacgacga gtcggtcaat gcccgcttct tgaagcgtat cgaagaacag
     1261 gtcgaacgac tcaaccagtt gattctcgac ctcctctccc tcgcccggat cgagtcgggc
     1321 caggagattt tcgagcacgg gccgctttac ctggtaccgg tcttggaatc gtgcgtggag
     1381 tcgcaccgcg accgcgcgca gaccaagaac ctcaatctca cgttcgatcc aggaaggctg
     1441 gacgaagaga ccatggttct cgccgacgaa gaggccatcc gccagatcgt cgacaacctg
     1501 gtggacaacg cgatcaagta cacaaccgag ggaggctccg tatcgatcac ttgccatagt
     1561 gaccagaatt cagccagcat cgaggttgca gacaccggca ttggaatccc ccgggccgat
     1621 ctgccacgga tttttgagcg tttctaccgc gtggacaagg cgcgtagccg cgagctgggt
     1681 ggaaccggcc tggggctgtc gatcgtcaag cacttgacgc aatcgatcgg gggccagatc
     1741 gacgtgacca gccggctcgg ttctggctcg acatttattg ttcacttacc ccgttgcctc
     1801 gtcgcggata tgcgaaaaca aggcgcggaa agaacctaat catgataaca actagcggcc
     1861 ggacgcgatt gttgattggt gggaccgagc ggctcgcgca ccagcagtca tgatcgaacc
     1921 agcagtcggc aggcgctggc atgaggtcct gctcttaaat ttgatcctcg tcgtgatcga
     1981 cttcccaatc gtaatctatt ctttagtctt acttttcttg ggtgttgctg tcggtaagct
     2041 atccccgtta agacccggcc ccgcctcggg gggccttgat taactacgtc ccaatcacaa
     2101 ctcaagagcg acggcttgat aggcggcggt gaggcgaggg tcgttgcgcc cccgcctcaa
     2161 gtcgtccacc cgttgcatac cgcgacgatg cctcgtctcc gaaccgccgc tcgttgcgcc
     2221 gtcgacacgc ttcgatcggg tgccacgcgc tattcgttga ccgcgatact ctcttcgacc
     2281 tgggccacct cctcgtagac cggtgttacc tgctcttcga cctcggtcgg aatgacctca
     2341 tcctgaaccg gtgcgacctg ttccgcgaac gcagtcggaa cgacctcgtc ctggaccggc
     2401 gtgacctgtt ccgccgcctc agtcgaaacg acctcatcct gagccagcgt gacctgctct
     2461 gggacctcag tgggaatgac ctcgtcctgg accggcgtga cctgttccgc caacgcagtc
     2521 ggaacgacct cgtcctggac cgtcgcgacc tgctcggcga cctccgtttc ttgcggcaca
     2581 gcgataaact cggcgttttc ggcaacgtcc gtgctctcct gcttggcctc ctcgaccggt
     2641 acttctaaca cttcattcat ggtgacgtgc tccgggatgc gatttgaaag tgatgtgagg
     2701 gaaacaccaa ctttaatatt accaatggaa cgtgtctttg catctttttt ttaaagtttc
     2761 tagagaattc gatacaaccg atgcggaggg ctaccgcttt gatgcctccc ggcaacatcc
     2821 ggggccaagg ttcaaaagca ggttacacct cagggggtgg ctggggtcga cgcgcgacgc
     2881 gggacgcccc cagatccgag gccgtcgcgc cgagggctcg ctggctgtcg ccatctcgac
     2941 cccagccacc cagctcttgt cacctgcaaa gttgctaaag ttgaaccatg cccgttcggg
     3001 gagtgcacag gtatcgcgag cttagattga gtccgacgcc caagaccact tcttgagcaa
     3061 agacgggagc gcaccagtct gtgggaccgg acggcgatat ggcaatgagc ccggtatcgt
     3121 cggcgtcgtt ccgcgcgatg cggcggcgta acgggccggc ccggctggcg tggccgaagg
     3181 tgccgatgtc agcaagcgat
//
LOCUS       DATAQL010001099         7884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4234652, whole genome shotgun sequence.
ACCESSION   DATAQL010001099 DATAQL010000000
VERSION     DATAQL010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4234652"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1449
                     /locus_tag="VHS97_18990"
     CDS             301..1449
                     /locus_tag="VHS97_18990"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3450348.1"
                     /translation="MRTHRDLRGFTLIELLVVIAIIAVLIALLLPAVQSAREAARRAQ
                     CTNKLKQIALAVNNYENTSSAIPPSAVLSTTQNYQDQGVLCRILPYLEQNADYNSINF
                     MYGVRGVWQTGGGWNAPPMDENVWAGDWGRSNATANITYVDTFLCPSDPNNGGNNQFF
                     INGQQRLICTTDYYWNVGKSRFFSGGVVNGPSYSPGAIDPNSPGGPDASKTITMASFT
                     DGTSNTAIMSESVQSNAGLEQDGLGMVYAGPSWNQFVGQGTATSPPDFLAAQFCQNTP
                     KLAHDYWWKGEFALSGGHNIYSHTQTPNRRSCYWTNVTGNFPDVSSDQDYAGATQTMV
                     AASSYHPGGVNVAFMDGSVHFVKNSVNFASWYALATPAGGEVISSDSY"
     gene            1579..2748
                     /locus_tag="VHS97_18995"
     CDS             1579..2748
                     /locus_tag="VHS97_18995"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450349.1"
                     /translation="MNASARAQPTANREARPTARRRFGLGSSRSLRIVFGLAIAGILL
                     GPSMWFLTQFDPLGHAGAAYGQHQYLSALRAAQNHLKWFPTDRRASLMVARCLMRLGR
                     APEAEAFYQKAAPLDLRDAQVRAYNLVNLNEPKQAALAYDELLANWPGDVLALKRLAA
                     IRMGLKQWRDVLKIADRLIANSDERVAGNTMAAIAHHELKHYDQAITSALRVLELDHD
                     LKRMPLPRTLFWNNLAIDLIAVGRTDEARRYLTDALAHSQDAGLMELVGLTYSQQGAL
                     DEAERCWRQAESWDAENADVCLDLGRLALSRNRWGEAVSYLKRAADRSADAVEPLYNL
                     SQAYRMLGNATEAERYRQLADLRRRARPAATGMGADADSESYADVRRAGARELPR"
     gene            2745..4013
                     /locus_tag="VHS97_19000"
     CDS             2745..4013
                     /locus_tag="VHS97_19000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450350.1"
                     /translation="MTSRDFECRSMCLGVLLLALLIAGAVLVVVSRRKPSASLDGLQP
                     LLATRRFDQAESLIREYLKIHPASVQGNMLMAQVALERDDQKPRLALDHLGRINARDH
                     ATRAIVMLNLGKAYSALGWNDRAEMAWKAALKLEPRVPEAGWNLLSLYYVEGRRAEAH
                     RFGMALRVTEPDPRDRAQLLLELVRHDAQPLGPDSLMLTLEPIVRKYPRDLHMAIALG
                     LALVRNSRVDEGLSNLRDLAERFALDPDARDALLLGLDEAGKFDDLASEMDRLPSAMV
                     SDPQFDRHRGAIAQQRQDWPVAAEQYESAWRRDPSDFRVLYRLSRALRMVGRLADADA
                     FDQKVHAAQDAKDRILPLYEDANAVKTLGTAPHADIYHRLANLRERMGRDDEAIAWHS
                     MVLEHQPDDKESKAAIARLNAAKAEITASH"
     gene            complement(4050..5237)
                     /gene="pheA"
                     /locus_tag="VHS97_19005"
     CDS             complement(4050..5237)
                     /gene="pheA"
                     /locus_tag="VHS97_19005"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX3450351.1"
                     /translation="MTRKPSPRKPPARGSKDRGSSPKSSDSKRHPSLVGLRGEIDRLD
                     QNLVTLLNRRAEIASQIGRIKHDQGLEVWSAARENEVISQALAASPGPLPAETLRIIF
                     RELMSGSRSLQRTLHVAFLGPKYSYSHLASVAKFGEAVEHVPVGSIAAVFEDVNRRHV
                     EFGLVPLENSTDGRVADTLDMFVKLPNIKIRAEVRLRIHHCLLGRSDWSQIRRVYSKS
                     QALSQCRNWLGKNLPQATKVEVVSTAAAAELAQREEFAAAVASRAAASAYQLNVLAEN
                     IEDQPHNVTRFAVIGDRVEERTGRDRTAIMVRLANQAGSLCRTISPFEKLGVNMTWIE
                     SFPIPGPIVKGDDTNQSYLFFIDVEGHQTDPVIQQALEAVRKRSERIDIIGSYPRSVC
                     VET"
     gene            5842..7773
                     /gene="dnaK"
                     /locus_tag="VHS97_19010"
     CDS             5842..7773
                     /gene="dnaK"
                     /locus_tag="VHS97_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX3450352.1"
                     /translation="MSEGEKIIGIDLGTTNSVVAVMEGGDVTVIPNQEGSRLTPSVVA
                     FTPKGDVLVGDPAKRQAITNPTETIYSIKRFMGRRHDEVKTEEKMVPYHIVGGPNEFV
                     KVHTNKKDYTPPEISALILRKLKEAAESYLGHKIRKAVITVPAYFNDSQRQATKDAGQ
                     IAGLEVARIINEPTAAALAYGLDKKKNEKIAVFDLGGGTFDISILDVADGVFEVLSTN
                     GDTHLGGDDWDEALMNHIAGEFKKEQGIDLRKDQMALQRLKEAAEKAKKDLSFQAQAD
                     INLPFITADASGPKHLTMTISRAQFEKLTDSLFERCRGPVLKALDDAKLKPSNIDEVV
                     LVGGSTRMPRVQQIVKEIFGKEPHKGVNPDEVVAVGAAIQGAVLTGEVKEVLLLDVTP
                     LSLGLETKGNVMTVLVPRNTTIPTEKKEVFTTADDNQTAVTIKVYQGERPMAPDNRLL
                     GEFNLEGIPPARMGVPKIEVAFNIDANGILNVTARDQGTGKEQTIKIQSSGGLDKDEI
                     ERMKRDAESHAAEDKKRRELAEARNSAEQRVYQLEKLMEESKDKLSESDQAALRAAID
                     KVNETKKQDDPAAINRALDELQRASQAMAEHLYAGAKPGPGPGAGPEAGPPPGGASPN
                     GGPGGKPEDVIDVEFEEKK"
BASE COUNT         1665 a         2360 c         2401 g         1458 t
ORIGIN      
        1 gggtgtgcca gggtgcggcg gaaatgccaa cgagcgcgaa ttcttggact ccgcgccgcc
       61 gggggagccc tcaccgtttc cgaacgaaag cgtcgcccaa cggaaggcga ggacacggag
      121 tgagccgtat tcggttcgga agaaaggcca atcgccaggt cagaaggccg cggtcaaaga
      181 cgctgctaac tcgagcaaac cttgaaacgc tatcgcgccc tcttcgacat cacaagctca
      241 taaaggcgtg cggatagtta taattccaat ttgatttcgt tttcacagga gctgcagaac
      301 gtgagaactc atcgtgatct gcgaggattc accctgatcg agctgctggt cgtgatcgcc
      361 atcatcgcag tcctgattgc actgttgctc ccggccgtcc agtcggcccg cgaagccgcg
      421 cgccgcgccc agtgcaccaa taagctgaaa caaatcgcat tggccgtcaa caattacgag
      481 aacaccagca gcgcgattcc accatcggcc gtgctctcaa cgacccagaa ctaccaggat
      541 cagggcgtgc tgtgccgcat cctgccttat ctcgaacaaa atgccgacta caactcgatc
      601 aacttcatgt acggcgtgcg cggtgtctgg caaacgggtg gcggctggaa cgcgcctccc
      661 atggacgaaa acgtctgggc cggcgactgg ggacgcagca atgcaaccgc caacatcacc
      721 tacgtcgaca cgtttctctg cccctccgat cccaataacg gtggcaacaa ccaattcttt
      781 atcaacggtc agcaacggtt gatctgtacg accgattatt actggaacgt gggaaaatcg
      841 cggttcttca gcggcggagt ggtcaatggt ccctcttaca gtcccggcgc catcgaccct
      901 aactctcccg gcggccccga cgccagcaaa acgatcacca tggcgtcgtt cactgacgga
      961 accagcaata ctgcgatcat gagtgagtct gttcagagca acgccggcct ggagcaagac
     1021 ggactcggaa tggtgtacgc gggcccctcg tggaaccaat ttgtgggcca gggcacggct
     1081 acatcgcctc ccgactttct ggcggcccaa ttttgtcaga acaccccgaa gctggcgcat
     1141 gactattggt ggaagggcga gttcgccctt tcgggtggac acaatattta ctctcatacc
     1201 cagacgccga acaggcgatc ctgttactgg accaatgtga ccggcaactt tccagacgtg
     1261 tcaagcgatc aggattatgc cggcgccacc cagaccatgg ttgccgccag ctcataccac
     1321 cccggcggcg tgaacgtcgc gttcatggac ggctccgtcc atttcgtcaa gaattcggtc
     1381 aacttcgcga gctggtacgc actggcgacc cccgcggggg gagaagtcat tagcagcgac
     1441 tcctattgaa aaccactctc ctgggtgttc acgacgcgcc aacccactga tccacgatcg
     1501 gcgacgcctt catgtccgtg aagatggtgc gggcgtcttt gattgtctca tcgccggacc
     1561 aggcgttacg atgtattcat gaacgcttcc gcacgagccc agcccacggc caatcgagaa
     1621 gcacgcccga cggcgcgaag aaggtttgga cttggctcaa gccggtcgct ccgcattgtt
     1681 ttcggtctgg cgatcgctgg tattctgctc ggcccgtcga tgtggttttt gacgcaattt
     1741 gaccctctcg ggcacgcggg ggcagcgtat gggcagcatc aatatctgtc ggcgctccgg
     1801 gcggcccaga atcacctcaa gtggttcccg accgatcgac gtgcttcgct gatggtcgcg
     1861 cgctgcctaa tgcggttggg gcgggccccg gaggccgaag ctttttacca gaaagccgcg
     1921 ccccttgacc tcagggacgc ccaggtgcgt gcttataacc tggtcaacct caatgagccg
     1981 aaacaagccg ccctcgctta tgatgagcta ctagcgaact ggcccggcga cgtccttgcg
     2041 ctcaagcggc tcgcggcaat tagaatgggg ttgaaacagt ggcgagatgt cctcaagatc
     2101 gccgatcggc tcatcgcaaa ttctgacgag cgtgtcgccg gtaacacgat ggccgcgatc
     2161 gcccatcacg aattgaaaca ttacgaccag gccatcacgt cggcgttgcg agtgctcgag
     2221 ttggaccacg acctcaagcg gatgccgctg ccgcgaacgt tgttctggaa caatctggcc
     2281 atcgacttga tcgccgtcgg ccggactgat gaagcacgga gatacttgac cgatgcgctg
     2341 gcccactcac aggacgctgg cttgatggaa cttgtggggc tgacctattc gcaacagggg
     2401 gcgctcgacg aggcggagcg ctgctggcgg caggccgaga gctgggacgc ggaaaatgcg
     2461 gacgtctgtc tcgacttggg ccgcctggcc ttgagccgca accgatgggg cgaagccgtg
     2521 agctacttga aacgcgccgc cgatcgatca gccgatgcgg ttgaacctct ctacaacttg
     2581 agtcaggcgt atcggatgtt aggtaacgcc acagaggcgg aacgctaccg gcaactcgcc
     2641 gacctgcgtc ggcgggcccg tccggcggcg acgggcatgg gggctgatgc tgattccgaa
     2701 tcttacgcgg acgtccggcg cgccggcgcg cgagagctgc cgcgatgaca tcgcgtgact
     2761 tcgagtgtcg cagcatgtgc ttgggggtgc ttttgctcgc gctcttgatc gccggagcgg
     2821 tcttggtcgt tgtatcgcgc cgtaagccct ccgcgagcct cgacggcttg cagccgctac
     2881 tggccacccg gcgatttgac caggcagaaa gccttatcag agaatacctc aaaattcacc
     2941 ctgccagcgt tcaaggcaat atgctgatgg cacaggtcgc cctggaacgc gacgaccaga
     3001 aaccccggct tgccctcgat catctcgggc ggataaacgc gcgcgaccat gccactcggg
     3061 cgatcgtcat gctcaacctg ggcaaggcct actcggccct cggctggaac gatcgcgcgg
     3121 agatggcctg gaaggccgcg ctaaagctgg agcctcgcgt ccctgaggct ggctggaatc
     3181 tcctcagcct ttattacgtc gaaggccggc gcgctgaggc gcatcggttt ggcatggcgc
     3241 tgcgggtcac ggagcccgac ccgcgtgatc gcgcgcagct actcctcgaa ctggtccgcc
     3301 acgatgccca gccgctcggc cctgattcgc ttatgcttac cctcgagcct atcgtgcgta
     3361 agtatccgcg cgatctccac atggcaatcg ccttgggcct cgcgctcgtg cgcaacagcc
     3421 gtgtcgacga aggcctatcg aacctgcgcg acctggccga acgcttcgct ctagacccag
     3481 atgcccgaga cgcgttgcta ctgggcctcg atgaggctgg caagttcgat gacctggcca
     3541 gcgagatgga ccgtctcccg tcggcaatgg tgagcgaccc gcaatttgat cgccatcgcg
     3601 gcgcgatcgc gcaacaacga caagactggc ccgtcgccgc cgagcaatat gagagcgcat
     3661 ggcgacggga tccctcagat ttccgggtac tttatcggct tagtcgtgca ctccggatgg
     3721 tcggccggct ggcggacgcc gatgcattcg accaaaaggt acatgccgcg caggacgcga
     3781 aagatcggat cttgccgctt tacgaagatg ccaacgcggt caaaaccctg gggacggcgc
     3841 cccatgccga tatctaccac cggctagcca acctccgcga acgcatgggc cgggacgatg
     3901 aggcgatagc ctggcatagt atggttctcg aacatcagcc cgacgacaag gaaagcaagg
     3961 ctgcaatcgc gaggttgaac gccgcgaagg ccgagatcac cgcctcgcat tgagcgtctt
     4021 ctaatcaatt cacgaatacg cggttcgcct caggtctcga cacacacact gcgggggtat
     4081 gacccgatga tgtcgatgcg ctcggatctc ttgcgaacgg cctcgagcgc ctgctgaatg
     4141 accgggtcgg tttgatgtcc ctcgacgtcg atgaaaaaga gataggattg gttggtatcg
     4201 tctcccttga cgatcggtcc ggggatggga aatgactcga tccaggtcat gttcacaccg
     4261 agtttttcga atggcgagat cgtccggcag agggagcctg cctggttggc cagacggacc
     4321 atgatcgcgg tcctgtcccg gccggtccgc tcctcgacac ggtcgccgat gacggcgaaa
     4381 cgggtcacgt tatggggctg gtcctcgatg ttttcggcga gtacgttcaa ctgataagcc
     4441 gaggcggcgg cacggctggc cacggcggcg gcgaattctt cgcgctgggc gagctcggcg
     4501 gcggcggctg tagatacgac ctcgaccttg gtcgcttgcg gcagattctt gccgagccag
     4561 ttacggcatt gcgacagggc ttgcgacttg gaatacaccc gccggatctg gctccagtcg
     4621 gaccggccca aaaggcaatg atggatccgt agccgcacct ccgcccggat cttgatgttg
     4681 ggcagcttga cgaacatgtc gagcgtgtcg gcgacccgtc cgtccgtcga attctcgagg
     4741 gggacgagcc caaactcgac gtggcggcga ttgacgtctt caaagacggc cgcaatcgag
     4801 ccgaccggga catgttcgac ggcttcgcca aacttggcta ccgatgccag gtgactgtag
     4861 ctgtatttgg ggccgaggaa cgcgacgtgg agggtgcgct ggagcgagcg cgacccgctc
     4921 atgagctcgc gaaagatgat gcgtaaggtt tcggcgggaa gcggacctgg gctcgcagcc
     4981 agggcctgcg agatcacttc gttttcacgc gcggcggacc agacctccag gccctggtca
     5041 tgcttgatcc ggccgatttg cgaggcgatc tccgcgcgac gattgagaag cgtgacgaga
     5101 ttctgatcca gccgatcaat ctccccccgc aagccaacca agctcggatg gcgcttggaa
     5161 tcagacgatt tgggcgatga accccgatcc ttcgagccgc gggccggcgg tttgcgtggg
     5221 gacggcttac gggtcatgtg tcgactcggt gagaaacagt cggagcagtc gcaacgccag
     5281 tctctgcagg cagccggatg ccggacgcgc acttctaccc cgagagggcc gcccaagaca
     5341 ttcagacgca agcttttctc ggataagtaa ttaaaagaaa agaatggcac atgtcaagcg
     5401 cattcctctt tgttccttcg tcgatgtagt tcgcaaccgg ccgggacgag tgtattatca
     5461 gttgtcttga ccgcgccaag ggcgcaggca cgaacacgca ggctggcgtt ttgggtgcca
     5521 tttttgcagt gatggttgag catttggcca ggcactgtca ttttggcagc actcgaaata
     5581 gcccatcgat ggcttggaag acaatcgatc gacctaaagg attttgcgca aactatttgc
     5641 tgcgggttgc caagctttcg tattcggact ggcatggccg ttgctaaaga attatcgagg
     5701 ttggtctgtg cgagcattcc acgcacaagg tgagttacct gggttgcgga ctgcgagcac
     5761 gctgcggttg gatcgagtac attcgatcgt gggctctggc taggttgtcg tcactgctca
     5821 ccgtcggaaa ggagacgcgt cgtgtctgag ggcgaaaaga tcattggcat tgacttggga
     5881 acgaccaaca gcgtcgtcgc ggtgatggag gggggcgatg taacggtcat cccgaatcag
     5941 gagggaagcc gtctcacgcc gtcggtggtg gctttcactc ccaaggggga cgtgctcgtg
     6001 ggtgacccgg ccaagcgcca ggcgatcacc aacccgaccg agacgatcta ctcgatcaag
     6061 cggttcatgg gccggcgtca cgacgaggtc aagaccgagg agaagatggt cccctaccac
     6121 atcgtcgggg gcccgaacga gttcgtcaaa gtccacacca acaaaaagga ctacacgccg
     6181 ccggagatct cggcgctgat tctaagaaag ctcaaggaag cggcggagag ctacctgggg
     6241 cacaagattc gcaaagccgt gatcacagtg ccagcctact ttaacgacag ccagcggcaa
     6301 gcgaccaaag acgccggcca gattgccggg ctggaggtgg cgcggatcat caatgagccc
     6361 acggcggccg cgctggcgta tgggctcgac aagaagaaga acgagaagat cgccgtcttc
     6421 gacttaggcg gcggtacctt tgacatctcg atcctcgacg tcgccgacgg cgtctttgag
     6481 gtcctctcca ccaacggcga cacccattta ggtggcgacg attgggacga ggccctgatg
     6541 aaccacatcg ccggcgagtt caagaaagag caggggatcg acctccgcaa ggatcagatg
     6601 gccctccagc gcttgaaaga agccgccgag aaggccaaga aagacctgtc gtttcaggcc
     6661 caggccgata tcaacctgcc gttcatcacg gccgacgcct ccgggcccaa gcacctcacg
     6721 atgactatca gcagggctca gttcgaaaag ctcaccgaca gtctcttcga gcgatgccgc
     6781 ggcccggtat tgaaagccct cgatgatgcc aagctcaagc cgtccaatat cgacgaggtt
     6841 gtcctggtcg gtggctcgac gcgcatgccg cgcgtgcagc agatcgtcaa ggaaatcttc
     6901 ggcaaagagc ctcacaaggg cgtcaacccc gacgaggtgg tcgccgttgg cgccgccatc
     6961 cagggggccg tcctcactgg cgaggtcaaa gaggtgctcc ttctcgacgt gactccccta
     7021 tcgctgggcc tggaaaccaa ggggaatgtc atgacggtct tggtcccccg gaacaccacg
     7081 attcccacgg agaaaaaaga agtcttcacc acggccgacg ataaccagac cgccgtcacg
     7141 atcaaggtct accagggcga acggccgatg gctccggaca atcggctctt gggcgaattc
     7201 aacttggagg ggattccccc tgctcgcatg ggcgtaccca agatcgaagt cgcgttcaac
     7261 atcgacgcca atggaatctt gaacgtcacc gcccgcgacc aggggacagg caaggaacag
     7321 accatcaaga tccaatcgtc ggggggcctc gacaaggatg agatcgagcg gatgaagcgc
     7381 gacgccgagt cgcatgccgc tgaagacaag aagcggcgcg agctcgccga ggcgcgaaat
     7441 agtgccgagc aacgggtcta ccagctcgag aagctcatgg aggaaagcaa ggacaagctc
     7501 agtgagtccg atcaggcggc cctgcgcgcg gccattgaca aggtcaatga gaccaagaag
     7561 caagacgacc cagcggccat caatcgtgcg ctcgacgaat tgcagcgtgc cagccaggcg
     7621 atggccgagc atctctatgc cggcgccaag cctggacctg gacctggagc cggacccgaa
     7681 gccggacctc cgcccggcgg cgcctctccc aacggcggcc ctggcggcaa acccgaagac
     7741 gtcatcgacg tcgaattcga agagaagaag tgataaggca cgcaaaaacc gttaccgccg
     7801 cttgacgtgt taattctcag tccatcttca gttttttctc cttcccccct gtgggggagg
     7861 gatagggtgc gaggcgggca agtg
//
LOCUS       DATAQL010001100         4402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3320175, whole genome shotgun sequence.
ACCESSION   DATAQL010001100 DATAQL010000000
VERSION     DATAQL010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3320175"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VHS97_19015"
     CDS             complement(<1..743)
                     /locus_tag="VHS97_19015"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX3450353.1"
                     /translation="MAEVNDRKMTAHECSLCSPVSRRDFVKAVSMGALAGAVPFIGRT
                     AAAAGPTQVGPTTSAAAETAVARFYKTMTPEQRKMICFEFDHPLRKRVANNWHIVKPT
                     IDNLSSEQKALCQEIMKNLCSEDGYERFMKQMEDDDGGFENYSVAVFGEPGTDKPFEW
                     VLSGRHDTLRADGNSVEGVAFGGPIFYGHAADGHNNEDSKHTNNVWWYQGDHANKIFK
                     TLDDKQQTQALVAKAEPDEPKSTSLKGNKL"
     gene            1019..2218
                     /locus_tag="VHS97_19020"
     CDS             1019..2218
                     /locus_tag="VHS97_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEX3450354.1"
                     /translation="MATFVLTDVSSDVWQENFSVDATAMGLAEGYAWSVTKHRLRGGR
                     RDGVDLIVVDNGALRFSIVPTRGMGLWNGWYDGNRLGWDSPVTDGPIHPSLVNLAAAG
                     GLGWLDGFDELLARCGLENNGAPYEVKSVNPDGSESNTTFGLHGKIANIPASYVAVHV
                     GSDDPHEIVVEGHVQESRLFGPQVRMVTRIGTVPGSNTLVVRDEFVNLKEQPVEIQIL
                     YHWNFGPPFLGPGSQFFAPIKSVTPRDARAQEGNGQLQIYGAPAPGSTEQVYFYELRA
                     GSAGQVGTLAMLRNHGGDKAVVLRFRNDQLPAFTLWKNTGGLRDGYVTGLEPGTNYPN
                     ARPFEKAHNRVVTLPVDGRYVAETTLEVLTTAQAVAAVETEIIALQGQHPPQVNPKPT
                     EPFAPAN"
     gene            complement(2245..>4402)
                     /locus_tag="VHS97_19025"
     CDS             complement(2245..>4402)
                     /locus_tag="VHS97_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX3450355.1"
                     /translation="TLQVRGLDCPKEVDSLTAALKDQSGVTRMGFDLIHGTMTIDYDD
                     GLIDPQTFIRLIDERAGLQSSLQGLVEPTALSWWSRYGRWVLTAGSGAALAGGTVISW
                     MGHRLGLEQAGADRLAVVGYALAVLIGGIGIFPRAARNLTRLRFDIDVLMGLAILGAM
                     ALGQWDEAATVAFLYGLSEALEGLSLDYARRSIRALLELAPPTAERIGADGKTQVVPA
                     SQIRRGDRVLVRAGDVLPVDGEVARGCSSVDQKTITGESVPVLRDEGDPVYAGTINGD
                     GTLEVTASGPVSDAVISRVIAQVRANQAARAPIERRISRFAAVYTPIVIALSLLAMLG
                     PPLFAWATGTASTGDGDVWRLWFGRGLVVLVIACPCALVIATPVAIVCGLAAAARGGV
                     LVRGGEYLEEIGRLRALAFDKTGTLTLGQPDVVEVVCAAGQENEDQVLRIAAALGDRG
                     GHVLGKAIARHARGMRLDVPVADNYTAIPGLGALGQVNAIEYHLGSHRYIDEAGLCGP
                     EFHAQLGQAESTAGTAVALTANSGPLGWIRLADRPRPEAARVLAQLHELGIQTIMLTG
                     DNARTAAAVARELGVGEERSELLPADKVSAIAELDARHGPTGMVGDGVNDAPALAAAK
                     VSFALGGISSGAALESADVVLLADDLSRLPWLIRLSRATLARIRQNITLALLTKAFVL
                     VLAVMGLANLWMAIAADVGTSLLVIANALLLLRPTE"
BASE COUNT          823 a         1415 c         1355 g          809 t
ORIGIN      
        1 agtttatttc ctttcagact ggtcgatttc ggctcgtcgg gctcggcctt ggccaccagg
       61 gcctgcgtct gctgtttgtc gtccagggtc ttgaaaatct tgttggcgtg atctccctga
      121 taccaccaga cgttgttggt gtgcttcgag tcttcgttgt tgtggccgtc ggcggcgtgg
      181 ccgtaaaaga tcggtcctcc gaaggccact ccttcgacgc tattgccgtc ggcgcgaagc
      241 gtgtcgtgcc gcccgctcaa gacccactca aacggcttgt cggtacccgg ctcgccgaag
      301 acggcgacgc tgtagttctc gaagccgcca tcgtcgtcct ccatttgctt catgaatcgt
      361 tcgtagccat cctcgctgca aagattcttc atgatttcct gacagagcgc tttctgctcg
      421 gagctcagat tgtcgatcgt gggtttgacg atgtgccagt tgttggcgac ccgcttccgc
      481 aacgggtggt cgaactcaaa acagatcatc tttcgctgtt cgggcgtcat cgtcttgtag
      541 aatcgtgcga cggcggtctc ggccgctgca gaggtggttg ggcccacctg ggttggtccc
      601 gccgcggccg cggttctgcc gataaagggc acggcgccgg cgagcgcgcc catgctcacc
      661 gccttgacaa aatcgcgtcg cgaaacaggc gagcacagcg agcactcgtg cgctgtcatt
      721 ttccggtcgt tgacctctgc catggaagcc gatctccttt tgtaacggga tgatatcggt
      781 gcggggaagg cgtgagcacg accgactaaa gggggatgat gcgaactcta gctggcacgg
      841 aaactccatc ccgccttccg tcttcggttt aggctaaacc gccaaccggg tatcgtcaac
      901 tgggcacggc gacattctgg acaccgcgtt ggcaatgtaa tggccgcgac gatattctga
      961 cgtggtcgcc gcgcagcacc gcgagcttgt tcgagccagt cattgaaagt cctttaccat
     1021 ggctacattc gtactcaccg atgtctcgag tgacgtgtgg caggaaaact tttcggtcga
     1081 cgcaacggcc atgggactgg ccgaaggtta cgcgtggtct gtgacaaaac accgcctgcg
     1141 aggcggccgc cgcgacggtg ttgacctgat cgtggtcgac aatggcgcac tacggttttc
     1201 gatcgtgccg acccgaggaa tggggctgtg gaatggctgg tacgacggca accgcttggg
     1261 ctgggactcg ccggtaacgg acgggccgat ccatccgtct cttgtcaacc tggcggcggc
     1321 cggcgggctg ggatggctcg acgggtttga cgaactgctg gcccgctgtg gactggaaaa
     1381 caacggcgca ccgtacgagg tcaagtccgt caaccccgat ggctccgaga gcaacacgac
     1441 cttcggcctt cacggcaaaa tcgccaacat ccccgcctct tatgtggccg tgcatgtggg
     1501 gagtgatgac ccacacgaaa tcgtggtcga aggacatgtc caggagtctc gcctgtttgg
     1561 accgcaggtg cggatggtta cgcggatcgg gactgttccg ggctccaata cactggtggt
     1621 tcgcgacgaa tttgtgaacc tcaaggaaca gccggtggag attcagatcc tctaccactg
     1681 gaatttcggt ccgccatttc tgggacccgg gtcacaattt ttcgcgccca tcaagtccgt
     1741 gaccccgcgg gacgcgcggg cccaggaggg gaacggccag ctccagatct acggtgctcc
     1801 cgcgcctggc tcgaccgagc aagtttattt ctacgagctg cgtgcgggtt cggcggggca
     1861 ggttggcacc ctcgccatgc tccgcaatca tggtggcgac aaggcggttg tcctgcgatt
     1921 ccgtaacgac cagcttccgg cgtttacact ctggaaaaac accggcggac tgcgcgacgg
     1981 ctacgtgacg gggctcgagc cagggacgaa ctatcccaac gcccgcccgt tcgagaaggc
     2041 ccacaacagg gtcgtgaccc tgccggtcga cggtcgttac gtagccgaaa cgaccctgga
     2101 agtgctcacg acggcccagg cggttgcggc cgtcgagacc gagatcatcg cactccaagg
     2161 gcaacatccg ccgcaagtaa atccgaaacc aacggagccc ttcgctcctg cgaactgagc
     2221 gccgatccgt gtgccattgt tcttttattc agtcggccgc aacagcaaga gcgcgttggc
     2281 gatcaccaat agactcgtcc cgacgtcggc ggcgatcgcc atccagaggt tcgccagccc
     2341 catgacggcc agtaccaaga cgaaagcctt ggtcaagagt gcgagagtga tgttctggcg
     2401 gatgcgggcg agtgtggccc ggctgagtcg aatgagccag gggagccgag agagatcgtc
     2461 ggccaagagc acgacgtcgg cggattcgag tgcggctccg ctggagattc cgcccagcgc
     2521 aaagctgacc ttggccgcgg caagagccgg ggcgtcgttg acaccatcgc cgaccatgcc
     2581 ggtcggtccg tggcgggcgt cgagctcggc gattgccgaa accttgtcgg ccggcaagag
     2641 ttccgatcgc tcttcgccga ctcccagctc gcgcgccacg gcggcagcag tgcgggcgtt
     2701 gtcaccggtg agcatgatgg tctgaatgcc tagctcgtgc agttgcgcga gcacgcgcgc
     2761 cgcctcgggc cgaggtcgat ccgccaaccg gatccagccc agcgggcccg agttcgctgt
     2821 gagagccacg gctgttcccg ccgtgctctc ggcctgtccg agctgggcgt gaaactcagg
     2881 gccgcagaga ccggcttcgt cgatgtaacg atggctgccc aggtgatact cgatcgcgtt
     2941 gacctggccc agggctccga ggcccggaat cgcggtgtaa ttgtcggcga ccggcacgtc
     3001 caggcgcatg ccgcgggcat gccgggcgat cgccttgccg agcacatgcc cgccgcgatc
     3061 gcccagagcg gcggcgatcc tcagcacttg atcttcattt tcctgtccgg ccgcgcagac
     3121 cacctcgacg acgtccggtt gaccgagggt cagagttccg gtcttgtcga aggccagcgc
     3181 gcgcaggcgg ccgatttcct cgagatattc acctccgcga accaacaccc ccccccgcgc
     3241 cgccgccgcg agaccgcaga cgatggccac cggagtcgcg atcaccagag cacaaggaca
     3301 ggcgatcaca agtacgacaa gtccacgccc aaaccaaagt cgccacacgt cgccgtcgcc
     3361 ggtactcgcg gttcccgtgg cccaggcgaa aagcgggggg ccaagcatcg ccagcaggga
     3421 tagggcgatg acgatcggcg tataaaccgc ggcaaaccgt gaaattcgcc gctcgatcgg
     3481 cgcgcgcgcg gcctgatttg cgcggacctg ggcgatgacc cgagaaatca ccgcgtcgct
     3541 gacggggcca gaggccgtta cttcgagcgt gccgtctcca ttgatcgtcc ccgcgtagac
     3601 cgggtcgccc tcatcgcgga gcacgggcac cgattcgccg gtgatcgtct tttgatcgac
     3661 gctcgagcac cccctagcca cctcaccgtc gaccggaagt acatcgccgg cccgaaccag
     3721 aacccgatct ccccggcgaa tctggctcgc cggcaccacc tgcgtctttc catcggcgcc
     3781 gatccgctcg gctgtcggag gagccagctc caaaagggcc cgaatcgacc gccgcgcgta
     3841 gtcgagactc agcccctcca aggcctccga cagaccatac aggaaggcca cggtcgcggc
     3901 ctcgtcccat tgtcccaggg ccatcgctcc caggatcgcc aatcccatca aaacgtcgat
     3961 atcaaaccgc aaccgcgtga gattgcgggc ggctctagga aagatcccga ttccgccgat
     4021 caacaccgcg agcgcgtagc cgaccactgc caggcgatcc gcaccagctt gctcaaggcc
     4081 gagccgatgg cccatccagg aaatcacggt accgcccgcc agggccgcgc cagaaccggc
     4141 cgtcaagacc cagcgcccat accgcgacca ccaggatagg gcggtgggtt ctaccagccc
     4201 ttgcagactc gactgcagcc cggctctctc atcgatcaaa cgaatgaatg tttgcggatc
     4261 gatcagtccg tcgtcgtaat caatcgtcat cgtcccgtga attaggtcga agcccatgcg
     4321 ggtgacgccc gactgatcct tcagcgcggc ggtgagcgaa tcgacctcct tgggacagtc
     4381 aaggccgcga acttgaagcg tg
//
LOCUS       DATAQL010001101         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4385460, whole genome shotgun sequence.
ACCESSION   DATAQL010001101 DATAQL010000000
VERSION     DATAQL010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4385460"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2362)
                     /locus_tag="VHS97_19030"
     CDS             complement(<1..2362)
                     /locus_tag="VHS97_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3450356.1"
                     /translation="MKCHLACALVVSTLLSAVRGADLPPRGAELPTRALPVVAEGWTI
                     TMALEAPEILFPTTIVATANGTVYLGSDPMDMPGPPTEPIDRVLAVKNGKVTVFADKL
                     WSVMGLEWIDQTLYVVHAPFLSALRDTDGDGRADARVDLVTGLGPKLPGSSGINDHIA
                     SGVRLGMDGFLYISVGDKGIPRAVGRDGRAIQLYGGGVIRVRPDGTDLEIVSTGERNP
                     LSVALSATDEIFTYGNDDDSKKWPNSLTHHIFGGHYGYPYQFLTAPHRALPIMAGDFG
                     GSGAQGICYNEDGLPAEYRGNLFFCDWGRQTVFRFEVKKSGGTFALIKRTALVSKGDV
                     TDFRPFSLAVANDGGGLWLVDWAYNGWLASGPRTGRLYRLQYTGSGVAARAPRPTGAD
                     SIERLKALDHPALAVRLESQRILQKKGAAAVPLLVERLKAGGAETGRMHAVWALDAIG
                     GDDARRAIGEVFADPSPRVRLQAARSAGIRGDRTVLRDLIGLLADRNPAVRREAAIAL
                     GRLEDRSAAIPLYAALGDSDPFAAWSVRQAIRRIGAWDKSALIEALLDERRLESALRL
                     TDESWALPVVDALIEALSKTGAAPVRGRIVANLAGLYRRYPEWSGQWFGTNPLAGEFP
                     RRTADWSPDGMNQVLRGLSLALADPASSVRFQAIVGMSEVGPAALPQLRSALIKEPDA
                     TNQAVLVEALGTLSDPMASPILAAIVRDLGRAEAVRVAAVKALAGARDAQSLRARLAL
                     LYDANAPAPLVALALPDLARMGFLPPNDLASFLEHPAPPVRAAAILS"
     gene            2361..>2575
                     /locus_tag="VHS97_19035"
     CDS             2361..>2575
                     /locus_tag="VHS97_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450357.1"
                     /translation="MCQSFCLVEPLQGLRHEPRARAENSREWNASFTPGRSSIAAGLV
                     IDRWTGQNCSVEPARPQREDGLIDLPRV"
BASE COUNT          514 a          840 c          797 g          424 t
ORIGIN      
        1 agctgagtat cgctgcggcc cgaaccgggg gcgcggggtg ttccagaaac gatgccaggt
       61 cattgggagg taaaaagccc attcgcgcca ggtccggcaa cgcgagcgcc accaagggcg
      121 ccggcgcatt cgcgtcataa agcaaggcca ggcgggctcg caatgattgg gcatctcgcg
      181 ctcccgccag ggccttgacg gccgccacac ggacggcttc ggcccgacca agatcgcgca
      241 cgatcgccgc caggatcggc gacgccatcg ggtctgacag agttcccaga gcttcgacaa
      301 gcaccgcctg gttggtcgcg tcgggttcct tgatcaaggc cgatcgtaat tgcggcaaag
      361 ccgccgggcc cacttcgctc atgccgacga tcgcctgaaa ccgaaccgag cttgcgggat
      421 cggcaagagc gagcgacaga cctcttagca cctgattcat tccgtcgggg gaccaatcgg
      481 ccgtgcgcct cgggaattcc ccggcgagcg gattcgtgcc gaaccattgg ccggaccact
      541 cgggatagcg gcgataaagc ccggccagat tggcgacgat ccgaccacgc accggggccg
      601 cgcccgtttt tgagagagct tcaatcaagg cgtccacaac cggcaaggcc cacgattcgt
      661 cggtgagccg cagagcggat tcgagccgcc gctcgtcgag caaggcctcg atcagggcac
      721 ttttatccca ggcaccgatc cggcgaatcg cctggcggac cgaccaggcc gcaaagggat
      781 cggagtcacc aagtgccgcg tagagaggaa tagcggccga gcggtcttcc agccgcccaa
      841 gggcgattgc ggcttcgcgc cgcacggccg gattacgatc agccagaaga ccgatcaaat
      901 ctctaagcac ggtccggtcg ccacggattc cggccgatcg tgccgcctgg aggcgaacgc
      961 ggggcgaagg atcggcaaaa acctcgccga tcgcccgccg agcgtcgtcg ccgccgatgg
     1021 cgtccagagc ccaaacggcg tgcatccttc ctgtctcggc gccccctgct ttcagccgct
     1081 cgaccaaaag tggaaccgcc gccgcccctt ttttctgaag aatgcgttgc gattcgaggc
     1141 ggaccgcaag cgcagggtga tcgagcgcct ttaagcgctc tatcgagtcg gcgcccgtcg
     1201 gacggggcgc tcgcgccgcc acgcccgagc ctgtgtactg cagccgataa agccggccgg
     1261 tgcgcggacc gctcgcgagc caaccgttgt acgcccagtc aaccagccag agaccgccac
     1321 catcgttcgc caccgccagc gaaaagggcc gaaaatcggt cacgtcccct ttggatacca
     1381 gcgccgtgcg cttgatcaga gcaaacgttc cacccgattt cttgacctcg aagcgaaaga
     1441 ccgtttgacg accccaatcg caaaagaaaa gattgccccg atactcggcc ggcagaccgt
     1501 cttcgttgta acagatcccc tgcgcacctg acccgccgaa gtcacccgcc atgattggca
     1561 atgcccggtg cggggcggta agaaattgat agggatagcc atagtgcccg ccgaagatgt
     1621 ggtgagtgag gctattgggc catttcttgc tgtcgtcgtc gttgccgtac gtgaagatct
     1681 catccgtcgc cgagagtgcg accgaaaggg gattgcgctc gcccgtcgat acgatttcca
     1741 ggtcagtgcc atcgggcctg acacgaatca cgccgccgcc ataaagctgg atcgcccggc
     1801 catcgcggcc gaccgctcgc ggaattccct tgtcgcctac cgagatatac aggaatccgt
     1861 ccatccccag gcgaactccc gaggcaatat gatcgttgat cccgctagag ccaggcagct
     1921 tgggcccgag tcccgtcacc agatcgacgc gcgcatcggc cctgccatcg ccatcggtgt
     1981 cgcgcaaggc cgagagaaac ggggcgtgaa cgacatacaa agtttggtcg atccactcga
     2041 gccccatcac gctccacagc ttgtcggcaa agaccgtgac cttgccgttt ttgaccgcca
     2101 gaacccgatc gatcggctct gtgggagggc ccggcatgtc catcgggtcg gacccgagat
     2161 aaaccgtacc gttggccgtg gcgacgatcg tggtgggaaa gaggatctcg ggcgcctcga
     2221 gagccattgt gatcgtccaa ccctcggcaa ccaccgggag tgcacgggtt ggaagctcgg
     2281 cgccgcgagg gggcagatca gcccctcgga ccgccgacag caaggtcgaa acgaccaatg
     2341 cacaggccaa atggcatttc atgtgtcaat ccttttgtct cgttgagccg ctccaagggc
     2401 tgcggcacga gcctcgtgcc cgtgcggaga attctcgcga gtggaatgcc agtttcacgc
     2461 ccggccggtc gtcgatcgcg gctggtctag taatcgaccg gtggacaggg cagaattgta
     2521 gcgtggagcc ggcccggcct caacgcgagg acgggctcat cgatctgccg cgtgt
//
LOCUS       DATAQL010001102         7364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5151381, whole genome shotgun sequence.
ACCESSION   DATAQL010001102 DATAQL010000000
VERSION     DATAQL010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5151381"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2482)
                     /locus_tag="VHS97_19040"
     CDS             complement(<1..2482)
                     /locus_tag="VHS97_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3450358.1"
                     /translation="MFSRFFIERPIFANVIAIVTMLVGGVTLFALPIEQYPAITPPTV
                     QVTTSYPGASAKVLADTVASPIEQEINGVEGMLYMSSTSANDGSYTLTVTFEVGTDLD
                     KAQVLVQNRTAIALPRLPVEVQRQGLNTKKQSTNIIMAVALTSPDDSHDSLFLSNFAT
                     LRVKDVLSRIHGVGDIQVFGSSNYGMRIWIDPEKLKARSLTTIDVLAAIAEQNVQVAA
                     GQVGQYPSPAGQSFQYNVTTLGRLSDPEQFGEIIVKTVEDSPARMRLTRVKDVARVEL
                     GAQVYDQWCEIGGRPAAGVAVFQLPGANALEVARKVKEAMERLKVSFPPGVAYSIPFN
                     PTDYVDESIHEVYKTLVEAGALVLIVILVFLQDWRAVLIPATTVPVTIIGAFAAMAAL
                     GFTINMLTLFGLVLAIGIVVDDAIVIVENAVHHIDRGQMGPKEATIKAMSEVIGPIIG
                     ITLVLLAVFLPSAFLGGITGQLYRQFALTIAATALISAINAVTLKPAQCAVYLRASPA
                     RRNLFCRVFNRAYDRCEQAYAAIIVRLVRHTVAMMLLFVALASGTAWWFIQLPTGFMP
                     QEDQGYAILGVQLPDAASQVRTRAVVRKLSEILRETPGVENWFMIGGMSILDQAAASN
                     GAAFYVTFAPWSQRTGREELGQTGILKALAAKAKSIHEAMIFAFPPPAIQGLGVAGGF
                     QFQLEDRAGVGLKPLQQIADELIRAGNAQTGLKGLQTSFRAEVPIIYADVDRVKAKSL
                     GVPLSAIFGTLQGTLGSTYVNDFNKFDRTYQVRVQADQRFRLEPEDIRRLEVRDWQGQ
                     MVPLGTLVKIEKRSGPHIIPRYNLYPSAA"
     gene            complement(2502..3797)
                     /locus_tag="VHS97_19045"
     CDS             complement(2502..3797)
                     /locus_tag="VHS97_19045"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450359.1"
                     /translation="MATLLLNGAGGCGQSNQYVEPDRPEVTVTTPVRRSVTSYLDYTG
                     TTQAVARIDLRARVRGFLKQKLFKEGSEVKAGQLLLVIDEETFQIRLEQAMAKQEEAE
                     SALRKAEESKAIEVAQAQLELDLSQLTLAHLDEARNRQLLNRRAGAKEDLDKSEATRK
                     KIEAQIKADRANLEQIEANYAINILTAKSGLRGAKAEVKNAQIDLGYCRLTAPIDGRI
                     SRSEVDVGNYVGDGFATVLATIVKTDPVRAFINVSENDLLRIDRMVRQGKRADHRNAT
                     LPMEMALADETGYPHLGVADYTDPVVDAGTGTVRIRGVFPNPDGAIVPGLFVRVRLPL
                     ESRENALLVPSRALGSDQSGEYLLVVGKDDVVVRRGVKVGAEVDGMRVVDGEIGPSDK
                     VVVDGLLQARPGTSVVPKPQEEPPAVAAALTTPGSGSQP"
     gene            4126..4968
                     /locus_tag="VHS97_19050"
     CDS             4126..4968
                     /locus_tag="VHS97_19050"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3450360.1"
                     /translation="MYTIGDFSKITGLTVKTLRFYHEQGLLMPSAIDDKTGYRYYDRS
                     RVETARVITHLKSLDLTIDEIGAILRDAKDDADLRDVMERQKALLETKIKRYRTILNS
                     VNQFLAAEEETRKLMTQSTFQVEEKTVEPLLIAGIRMKGRYADSGTGFSRIGKSLGRY
                     ICGKPMLLHYDAEFRQDDADFEACMPVRTEKQVDGICIRRIAGGRCVSCLHQGPYDQM
                     GRSYAKILDYIRSKGYEVQMPTREIYLKGPGMIFAGNPRNYLTEIQIMIEDAAREKRA
                     ETGG"
     gene            5202..5507
                     /locus_tag="VHS97_19055"
     CDS             5202..5507
                     /locus_tag="VHS97_19055"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX3450361.1"
                     /translation="MLLTDTDTGLVIPGCGGLRKMRVADPKRGKGKWGGARVICLHIQ
                     ELDHVRLVTVYGKDKKDDVSADDKKPFRRFARILKDQARRRRLRGVRWPVAVPSHPG"
     gene            complement(5652..5894)
                     /locus_tag="VHS97_19060"
     CDS             complement(5652..5894)
                     /locus_tag="VHS97_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450362.1"
                     /translation="MAIVLPHALAERPTLHIARFVLHQYSFFSSTLTRNWIAIRWFQQ
                     LAADIGTPEDNLADLSYTGDADHPPCTVELQAAGFF"
     gene            5893..>7364
                     /locus_tag="VHS97_19065"
     CDS             5893..>7364
                     /locus_tag="VHS97_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450363.1"
                     /translation="MSVRTEDITRHIRQLFEAGSGVGLTDGQLLARFACRVEESGSNP
                     DSASAEAAFETLVARHGTMVLSVCRQVLGDLHAAEDAFQATFLVLIRRSGSFEVRACD
                     SLGPWLYGVAYRTAIKARQLAARRLARERAVALRATAASAAPAEYDDFRSLLHEEVSR
                     LPAKYRAPVVLCYFEGRTHDEAAGALHWPVGTVRGRLARARDLLRSRLTRRGCAPAAV
                     IGASALDQSARVVVAASLHDATVAMATKGAPVAVSVAALTNIMLKSLVMARMKFSAVA
                     ALVIALLGTGAGIVMQGALAVQPLQGTGPAAHQSGTEQAQPTIAGRFIVDALPKYALA
                     RLGNARFHNGDSVGRVLYTPDGKSLIAAGSRSPVRVLDSATGRIIREIGESKLKLRDI
                     ALSPDGKTLATIHDPAWLRLWDVATGRERRTWHQGKDEHYQHLAFSPDGKTVAAAVTT
                     FDEATRKNRVFVNLWDLASTTEHRRRLEGNWLSFNDLAFSP"
BASE COUNT         1406 a         2194 c         2297 g         1467 t
ORIGIN      
        1 gagcggctga gggatacagg ttgtagcgcg gtatgatgtg cggaccggac cttttctcga
       61 tcttcaccag cgtgcccagt ggtaccatct gtccttgcca gtcgcgcacc tcgaggcggc
      121 ggatgtcttc cggctccagc cgaaatcgct ggtcagcctg gacccgcact tggtaggttc
      181 ggtcgaactt gttgaaatca ttgacgtacg ttgatcccaa ggtcccctgc aacgtgccga
      241 agatcgcgct tagcggaacg cccaggctct tggcctttac gcggtcgacg tcggcataga
      301 tgatgggcac ctcggcacga aagctggtct gtaggccttt tagtcctgtc tgtgcgttgc
      361 cggcgcggat tagctcgtcg gcgatttgct gcagtggctt gaggccgacg ccggcgcgat
      421 cctcgagctg gaactgaaag ccccccgcca cgcccagacc ctgaatggcc ggcggaggaa
      481 aggcgaagat catcgcctca tggatcgact tggccttcgc ggcgagcgcc tttaagatac
      541 cagtctggcc gagctcctcg cggcccgtcc gctgtgacca gggcgcaaat gtcacgtaga
      601 aagccgcccc gtttgacgcg gccgcctggt cgaggatcga catgccgccg atcatgaacc
      661 agttctcaac gcccggcgtt tcacggagaa tctcactcaa cttgcgcacc acggcccggg
      721 tgcgcacctg ggaggcggca tcaggtagct gcacgcccag gattgcgtat ccttgatctt
      781 cttgcggcat gaacccggtc ggaagctgga tgaaccacca cgcggtgcca ctggcaagtg
      841 cgacaaagag cagcatcatc gccacggtat gcctgaccag acgcacgatg atggcggcgt
      901 aggcctgctc gcagcggtcg taggcccgat tgaacacccg gcaaaagagg ttgcgcctgg
      961 ccggcgaagc tcgcaggtag accgcgcact gcgctggctt taacgtcacc gcgttgatcg
     1021 cgctgatcaa tgcagtcgcg gcgatcgtca gcgcaaactg gcggtagagc tggccggtga
     1081 ttccacccaa aaacgcgctg ggcagaaaaa cggcgagtag caccagggtg atgccgatga
     1141 tcgggccgat cacttccgac atggccttga tggtcgcctc ttttgggccc atctggcccc
     1201 tatcgatatg gtggacggcg ttctcgacga tcacgatggc gtcgtcgacg acgatgccga
     1261 tcgctagcac gagcccgaaa agagtgagca tattgattgt gaagccgagg gccgccatgg
     1321 ccgcgaaggc cccgatgatg gttaccggta ccgttgtggc gggtatcagt acggctcgcc
     1381 agtcctgaag aaacaccagg atcacgatca agacgagggc gccggcttcg accagcgttt
     1441 tgtacacttc gtggatcgat tcatccacgt aatcggtggg attaaaggga atcgagtagg
     1501 caactcctgg ggggaatgag accttgagcc gttccatggc ctccttgacc ttgcgggcga
     1561 cctcaagcgc gttggcacca gggagctgaa agacggcgac gccggcggcc gggcggccgc
     1621 cgatctcgca ccactggtcg taaacctggg cgcccagctc cacccgcgcg acgtccttga
     1681 ctcgggtcag ccgcatccga gccggagagt cctcgaccgt cttgacgatg atctcaccaa
     1741 actgctccgg atcgctcagc cttcccaatg tcgtgacgtt gtactgaaag ctctggccgg
     1801 ccggcgacgg gtattggccg acctggccgg cggccacctg cacgttttgc tcggcgatcg
     1861 cggccagcac gtcgatcgtc gtcaagctgc gtgccttgag cttttcggga tcgatccaga
     1921 tccgcatgcc gtaattactg ctgccgaaca cctggatgtc gccgactccg tggatccggc
     1981 tcaagacgtc cttgactcga agggttgcga agttgctcag gaacaggctg tcatgactgt
     2041 cgtcgggtga ggtcagggcc acagccatga tgatgttagt ggattgcttc ttggtattga
     2101 gcccttgccg ttgcacctcg acgggaagtc tcggcagcgc gatcgcggtc cggttttgaa
     2161 ccaggacttg agccttgtcg agatcggtgc cgacctcgaa ggtgacggtg agggtatacg
     2221 acccatcgtt ggccgacgtg gaggacatgt agagcatccc ctcgacgccg ttgatttctt
     2281 gctcgatcgg cgaggcgacg gtgtcggcca aaaccttggc gcttgcgcct gggtaactcg
     2341 ttgtcacctg aacggttggc ggcgtgatcg cggggtactg ttcgatcggc agcgcaaaga
     2401 gcgtgacgcc accgaccagc atcgtcacga tcgcgatcac attggcgaag atcggccgct
     2461 cgatgaaaaa acgagaaaac atgagcgcat tccgatcggg ctcagggttg cgacccgctg
     2521 ccaggggtgg tcagggcggc cgcgacggcc ggcggctcct cttgcggttt gggaacgacc
     2581 gaagtgcccg ggcgtgcctg caagagcccg tcgacgacga ctttgtcgct agggccgatc
     2641 tcgccgtcca ccaccctcat gccgtcgact tccgcaccga ccttgactcc tcggcggacg
     2701 acgacatcgt ctttacccac gacgagcaga tactcgcccg actgatccga gcccagcgcg
     2761 cggctcggca cgagcagggc gttttctcgg ctttccagcg gcagacgaac ccgcacgaac
     2821 aatccgggaa cgattgcgcc gtcggggttg ggaaagaccc cgcgaatgcg caccgtgccg
     2881 gttccggcgt cgacgacggg gtcggtgtag tcggcaacgc caagatgagg atagccagtc
     2941 tcgtcggcca gcgccatttc catcggcagt gtggcgttgc ggtggtctgc tcgtttgcct
     3001 tgccggacca tccggtcgat tcgcagcaag tcgttctcac tgacgttgat gaaagcacgg
     3061 acgggatccg tcttgacgat cgtggccagg acggtcgcaa agccgtcgcc gacgtagttg
     3121 cccacatcga cctcgctgcg ggagatccgc ccgtcgatcg gggcggttaa ccggcagtag
     3181 cccaggtcga tctgggcgtt cttaacctcg gctttggcgc cgcgcaggcc ggatttggcg
     3241 gtgaggatgt tgatcgcgta attggcctca atttgctcga gattggcgcg atctgccttg
     3301 atctgcgctt cgatcttttt gcgggtggct tccgacttgt ccagatcttc tttcgccccc
     3361 gccctgcggt tcaagagctg gcgattgcgc gcttcatcca ggtgcgccag tgtgagctgt
     3421 gaaaggtcga gctcgagttg tgcctgggca acctcgatgg ccttggactc ctcagccttc
     3481 ctgagcgcgg actcagcttc ttcctgcttg gccatggctt gctcgagccg gatctgaaaa
     3541 gtttcttcgt cgatcaccag caagagctgg ccggccttga cctcgcttcc ctccttgaac
     3601 agcttctgct tcaagaatcc ccggaccctg gctctcagat cgatgcgtgc gaccgcctga
     3661 gtcgtgccgg tgtaatccag atagctggtc accgaccggc gcacgggagt cgtcacagtc
     3721 acctcgggac gatccggctc gacgtactga ttactctgtc cgcaacctcc ggcgccattg
     3781 aggaggagag ttgccacgac cagcagggtc gccggcgggc aaccgacgcg accagcgcaa
     3841 tttaccgaca cgcagctgtc ccccaatgga ctggttttcg gcacggcggc accggtcgcc
     3901 tcgctcacac gatccggtct acgaatcaaa gggatcgacg gccaatgctg acgagccggc
     3961 gacgatcaat tctccgttca cgaatcacct agtctaccct agatgctgcg gaccctccac
     4021 ttgaatatcc agttgactct ccccttgcgg gaggccttat cctgattcga cgtggtgatg
     4081 atggcgggcg gattgggtgg gatatcgtgg cggcacgagg acctcatgta cacgatcggc
     4141 gacttttcca agatcacggg cctgaccgtg aagactttac ggttctatca cgagcaaggg
     4201 ctcttgatgc cgtcggcgat cgacgacaag accggctatc gctactacga ccgctcgagg
     4261 gtcgagactg cccgagtgat aacccatctg aagagtctgg atctcactat cgatgagatc
     4321 ggcgcgatcc tccgcgatgc caaggatgac gccgacctgc gcgacgtgat ggagcggcag
     4381 aaggcgctcc tggagaccaa aatcaagcga tatcgcacga tcctcaattc cgtgaatcag
     4441 ttcctggccg cggaagaaga aacgaggaaa ctcatgacac aatcgacttt tcaggttgag
     4501 gaaaagaccg tcgagccttt gcttatcgcc ggcattcgaa tgaagggccg atacgccgac
     4561 tcgggcacag gcttttcccg aatcggcaag agcctgggtc ggtacatttg cggcaagccc
     4621 atgctgctcc attacgacgc cgagttccgg caggacgacg ccgacttcga ggcatgcatg
     4681 ccggttcgca ccgaaaagca ggtcgacggg atctgcataa gacgcatcgc tggcggacgc
     4741 tgcgtgtcgt gtttgcacca agggccgtat gaccagatgg gccgctcata cgccaaaatc
     4801 ctcgactaca tccgtagcaa gggttacgag gtgcagatgc cgacgcgcga gatctacttg
     4861 aagggaccag gaatgatctt cgcgggcaat ccgagaaact atctgaccga gattcaaatc
     4921 atgattgagg acgcggcgag ggaaaagcgt gccgaaactg gtgggtagat tgggcgaaca
     4981 aatcggtgtc agttcgactt tccacagggc gaacaaatcg gtgtcagttc gactttccac
     5041 agcgaacaaa tcggtgtcag ttcgactttc cacagagtcc gactgcttaa agctatccca
     5101 cactgtagcg tagaacgcga aagacgttta tcgagactgt cgggttcaca gactgggtcc
     5161 gcgagtatct gtccaatgag gctctttccg aattgcagaa agtgttgctg accgacactg
     5221 ataccgggtt agtgattccg ggttgtggcg gcctgcggaa aatgcgggtg gccgacccca
     5281 agcgtggcaa ggggaagtgg ggcggggcaa gggtgatctg tttgcacatt caagagctgg
     5341 accatgtacg cctcgtcacc gtctatggca aggacaagaa agacgacgtc tcagcagacg
     5401 acaagaagcc cttccgccgg ttcgcccgca ttctgaagga ccaggcccga cgccgtcggc
     5461 ttcggggagt gcgatggcct gtagccgtac catcacatcc agggtgatcg gatggcagtc
     5521 attggccaac gcaggtcgtg gcgacgcgcg acggcgtcca ccgttggtct cggacggggg
     5581 cttgatcgtc ttggacatga tgcaattctc tcacgtcagg tcgattcctc gggatcatag
     5641 cccatccgta cttaaaagaa cccggccgcc tggagttcca ccgtgcaggg cggatgatcc
     5701 gcatcgccag tgtaactcag gtccgctagg ttgtcttccg gcgttccgat gtcggccgca
     5761 agctgctgaa accacctgat ggcaatccag tttcgggtca aagtcgatga gaagaaactg
     5821 tattgatgca gcacgaagcg ggcaatatgg agagtaggac gttcagctaa cgcgtgcggg
     5881 aggacgatcg ccatgagtgt gcgcacggaa gatatcacgc ggcatatccg ccaacttttc
     5941 gaggcgggaa gtggagtcgg gttgaccgat ggtcaacttc tggcgcgatt cgcttgcagg
     6001 gtcgaggagt cggggtccaa tcctgactcg gcctcggcgg aggctgcatt tgagacgctc
     6061 gtggcccggc atgggacgat ggtcctctcc gtgtgccgtc aggtacttgg cgatctacac
     6121 gcggccgagg atgcattcca ggcgactttc ctcgtattga ttcgccggtc cggttcgttt
     6181 gaggtgcgcg cgtgcgactc gcttggtcct tggctctacg gtgtagctta tcggaccgcc
     6241 atcaaggccc ggcagctcgc ggcccgcaga ctcgcgcggg agcgcgctgt cgcactgcga
     6301 gcgacggcgg cgagtgcggc gccggccgag tacgatgact tccggtcgtt gcttcatgag
     6361 gaggtcagcc ggctaccggc gaaataccgc gctcccgtcg tgctttgcta ctttgagggc
     6421 cgcacgcacg acgaagccgc cggggcgctc cactggccgg tgggtaccgt ccgcgggcgt
     6481 ttggcccgag ctcgcgatct gctccggtcc cggctcaccc gccgcggctg cgcgcccgcc
     6541 gcggtgatcg gcgcctccgc cctagatcaa tcagcccgcg tggtagtcgc cgcgtccctt
     6601 cacgacgcga cggtcgccat ggccactaag ggcgcgccag tcgcggtcag cgtggccgct
     6661 ttgactaaca tcatgttgaa gagtctggtc atggctcgaa tgaagttcag tgccgtggcc
     6721 gctctggtta tcgcactgct cggaacgggc gcgggaattg tgatgcaggg ggcactcgcg
     6781 gtccagccgt tgcaagggac cggtcccgca gcccaccagt ccgggaccga acaggcgcag
     6841 ccgacgatcg ccggtcgttt tatcgttgat gcactgccca agtatgccct cgcgcgattg
     6901 gggaatgcgc gatttcacaa cggcgactct gtcggaagag tcctctacac tcccgatggc
     6961 aaatctttga tcgcagctgg gtcccgaagc ccggttcgcg tcctggattc ggccacgggg
     7021 cgaatcatcc gcgaaatcgg cgagtccaaa cttaagctcc gggatatcgc actctctccc
     7081 gacggaaaga cgctggcgac gatccatgac ccggcctggc tccgactttg ggacgtcgct
     7141 acgggccgcg agcggcggac gtggcaccag ggcaaggatg agcactatca gcatcttgcc
     7201 ttttctcctg acgggaagac tgtcgccgcc gccgtgacaa cgttcgatga agcgactcgg
     7261 aagaacaggg tcttcgtcaa tctctgggac ctggcctcga cgaccgaaca tcgtcggagg
     7321 ctggagggga attggctctc tttcaacgac cttgcgtttt ctcc
//
LOCUS       DATAQL010001103         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1487641, whole genome shotgun sequence.
ACCESSION   DATAQL010001103 DATAQL010000000
VERSION     DATAQL010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487641"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1983
                     /locus_tag="VHS97_19070"
     CDS             163..1983
                     /locus_tag="VHS97_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3450364.1"
                     /translation="MAISTSARDRATSGKAEEALRAKQIEQAEELLFSGPEATGFAKA
                     LFRGEFRGDALFPYPELPEKERGTVEQAVAAVTEFADTHIDAAAIDREADIPRSVVQG
                     LARLGVLGMAAPTQWGGQGFSQMGYCRIMEVIGGHCSSTAVFVNAHHSIGLRALVLFG
                     TPDQQARWLPPLASGEKLAAFALTEEQAGSDASNVQTSARPSDDGETYILNGSKRYIT
                     NGAIADVLTVMARTPDPRGGDSKVTAFLVTPDLPGFEVVEARMPKCGIRGTATARLAF
                     HDMPVPATNILGPLGKGLKVALTVLDFGRTTFGASCTGAAKVCLAAATRHAASRRQFG
                     RPLADLELIKKKLAYLAATAYAMEATTYQTAALIDRGAEDYMLETAILKVFTTEALWE
                     GVNETLQVFGGQGYFSNEPYERMMRDARINTIGEGANEVLKAFIAMVGMRDIAVGLKD
                     TLDGLKKPSSFLSTLWGFSRDHVGRLVRTPVVPVVNPMLRPMADSLGRRVARFGRSVE
                     RVLVTHREAILDREYVQERIADAAIALVTGACTLSRWDLSITQSRSTPAERTAAELYL
                     RMAQRRFDRSLQALSQNDDRLTTAAADAALAAWTDYQPSR"
     gene            complement(1991..2554)
                     /locus_tag="VHS97_19075"
     CDS             complement(1991..2554)
                     /locus_tag="VHS97_19075"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3450365.1"
                     /translation="MSTASVTTPALLTAEEFAERPDPGYPEELVRGRIVPMPMPKPRH
                     GEICNRAGRIFGNCSEDRDLGRVLSNDTGVITERGPDTVRGADVSFYSFARVPKGPLP
                     DNYLDVAPDLVVEVLAPSDRWPKVLAKVAEYLDAGTSVVLVLVDKRRIAHLFRADGTI
                     RMLGPDEELTIPDIFGDFQVRVGRFFE"
     gene            complement(2599..>2930)
                     /locus_tag="VHS97_19080"
     CDS             complement(2599..>2930)
                     /locus_tag="VHS97_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450366.1"
                     /translation="AFETDDFDVITQQHGTNERTLCGRVEISPRGVPEWDWGPYYPGG
                     TVQSKVIDGTLAGKMAFWGEIGHHGSDFIADQFLKEHSEYDWARGLIKDLKCQPWSRF
                     EVDIKDR"
BASE COUNT          554 a          903 c          900 g          573 t
ORIGIN      
        1 ctttgccagt gtcttctgaa ccaaggtcgc gcgaagaggg gtaagcttcg cgttcgtcag
       61 tcgccttctc ttcgcgcggc tagaggctac aattccagag taacgagccg aatctcgatc
      121 tcttcccgcg atttcactcg acgaatggag gagcctttcg ccatggcaat ttcaacctcg
      181 gcacgcgacc gcgcgacgtc gggcaaagcc gaagaagcgt tgcgggccaa gcagatagag
      241 caagcggaag agcttctgtt ttcaggccca gaagcgaccg ggtttgccaa ggcgctcttt
      301 cggggcgaat ttcgcggcga cgcgctgttc ccgtatccgg agctccctga aaaagagcgc
      361 gggaccgtcg aacaggcggt cgccgcggtg actgagtttg ccgatacgca catcgatgcc
      421 gcggccatcg atcgcgaggc cgatatcccg cgctcggtcg ttcaagggct ggcgcggctc
      481 ggcgtattgg gcatggcggc accgacgcaa tggggaggcc aagggttctc gcagatgggc
      541 tactgccgga tcatggaggt gatcggcggg cattgctcgt caacggccgt cttcgtcaac
      601 gcacatcact caatcggctt gcgagcgctc gtcctgttcg gcacccctga tcaacaggca
      661 cgctggctgc cccccttggc cagcggtgag aagctggctg cgtttgccct gaccgaagaa
      721 caggccggat ccgacgcgag caacgtccag acgagcgcgc gcccgagcga tgatggcgag
      781 acttatattt tgaatggcag caagcgctac attaccaacg gagcgatcgc cgatgttttg
      841 accgtcatgg cgcggacgcc cgacccgcgc gggggcgatt cgaaggtcac tgcatttctg
      901 gtcactcccg atttgcctgg gtttgaggtt gtagaggcgc ggatgcccaa gtgcgggatc
      961 cgcggcaccg caacagcccg gctggcattc cacgacatgc cggtcccggc gaccaatatt
     1021 ctgggaccgc tcggtaaggg gctgaaggtt gctttgaccg tgctggattt cggccggacg
     1081 acctttggcg cgagctgtac tggggcggcg aaggtctgtc tggcggctgc cacccgccac
     1141 gcggccagcc ggcgccagtt tggccgtcca ctcgccgacc tcgagttgat caagaaaaag
     1201 ctcgcctatc tcgccgcaac cgcgtacgcg atggaagcca cgacctacca gacggccgcc
     1261 ctcatcgacc ggggcgccga agattacatg ctcgagaccg cgattctcaa ggttttcaca
     1321 accgaagcct tgtgggaggg ggttaacgaa acgctacagg tttttggcgg ccagggctat
     1381 ttcagcaacg agccgtacga acgaatgatg cgcgatgcgc ggatcaacac gatcggggaa
     1441 ggagccaacg aggttctcaa ggctttcatc gccatggtcg gtatgcgcga catcgccgtc
     1501 ggactcaagg acacgttgga cggtctgaaa aaaccgagct cattcttgtc cacgctctgg
     1561 ggtttttcgc gcgaccacgt gggccgtctc gtgcggaccc cggttgtgcc ggtggtaaac
     1621 ccgatgctca ggccgatggc cgactcgttg ggacgccgcg tggctcggtt cggccgtagt
     1681 gtcgagcgag tcttggtgac ccaccgtgag gccattctgg atcgcgagta tgttcaggag
     1741 cgcatcgccg acgctgcgat tgcgcttgtg accggggcct gtacgctgtc gcggtgggat
     1801 ctctcgatca ctcagagccg gtcgactccg gcagagcgaa ccgccgccga actttacctg
     1861 cggatggctc aacgccgctt cgaccgatcg ctccaggcgc tcagtcaaaa cgacgatcgg
     1921 ctgacgaccg cggcggccga cgccgcgctc gccgcatgga cggactatca accgagccgc
     1981 tgacccgttt tcactcgaag aatcgtccga cccggacttg gaagtcaccg aatatatcgg
     2041 gaatggtcaa ctcctcgtca gggccgagca tgcggatagt tccatccgct cggaagagat
     2101 gtgcgatgcg ccgtttatcg actagcacca ggacgacgga cgtccccgca tcgaggtatt
     2161 ccgccacctt cgcaagcacc tttggccagc gatcgctcgg cgcgagaacc tcaacaacca
     2221 agtcgggcgc cacgtcaagg taattgtcgg gcaaaggccc cttgggcacg cgggcgaaac
     2281 tgtagaagga gacatccgca cctcgcaccg tgtcgggtcc gcgttcggta ataacgccag
     2341 tgtcattact gagcacgcga cccaggtcgc ggtcctcgga gcaatttccg aagatccggc
     2401 cggctcgatt gcagatttca ccgtggcgcg gcttgggcat tggcatcggc acgatccttc
     2461 cgcgcacgag ttcctccgga taccccgggt caggcctttc ggcaaattct tccgcagtca
     2521 gcaaggcggg cgtggtcacc gatgccgtgg acatggccca tttctccttc gttaattgga
     2581 ctttactgat tatcgcattt atcgatcctt aatatcgacc tcaaatcgac tccacggctg
     2641 gcacttcagg tctttgatca gtccgcgcgc ccagtcgtac tcgctgtgtt ccttcaggaa
     2701 ctggtcggca atgaagtccg agccatggtg gccgatctcc ccccagaacg ccatcttgcc
     2761 ggcgagtgtg ccatcgatca ccttggactg cacggtccca ccggggtaat aaggtcccca
     2821 gtcccactcg ggcacgccgc gcggggagat ctcgacgcgt ccgcaaagag tccgctcatt
     2881 ggttccgtgt tgctgcgtga tcacgtcgaa gtcgtcggtc tcaaaggcct
//
LOCUS       DATAQL010001104         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3166073, whole genome shotgun sequence.
ACCESSION   DATAQL010001104 DATAQL010000000
VERSION     DATAQL010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166073"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VHS97_19085"
     CDS             complement(<1..494)
                     /locus_tag="VHS97_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450367.1"
                     /translation="MRRALMPLGTFEPSSVPPLTPGSTGSTVERRVGVRVFLAQTIPR
                     RQKPVGRLSIGSVQERSLMTMDGRPGRREPGMVLTGRINAEAVESTAAVVGAPVPARA
                     VGVPESAFIPPTRSRSTSLRCTLEVIGRACAIAVIVCLVVSARARAQDKFPDGPISKI
                     EFEG"
     gene            complement(760..1569)
                     /locus_tag="VHS97_19090"
     CDS             complement(760..1569)
                     /locus_tag="VHS97_19090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3450368.1"
                     /translation="MQISQTAAVLTKFDSRPWEISTQDRPMTDSYRDDLAHIHDAGFG
                     GFAQAAAPVLVEALKAGGIDQGLVIDLGCGSGILSRAIYQAGYDVLGVDISAAMIALA
                     RQRVPRGEFRVQSLLKSELPPAVAVAAVGEAINYLFDPENTRSSLAKLLERIHRALQP
                     GGVFLFDAAEPGRVPGNGLARTYFEGNGWAVLVSSEEDRKRKFLTRRITSFRQVGEVY
                     RRDQEVHCQRLLPRSELAAQLRSIGFRVRVLRGYGRLRFGSGHVGLLARKP"
BASE COUNT          430 a          703 c          629 g          374 t
ORIGIN      
        1 ttcccctcaa actcgatctt ggagatcggg ccatcgggaa acttatcctg agcccgtgcg
       61 cgagccgata cgaccaagca gacgataacc gcgatggcgc acgcccgacc aatcacctcg
      121 agcgtgcagc ggagactggt agagcgcgat cgcgtcggtg gtatgaaggc ggattcgggt
      181 acgcccaccg cacgcgcagg cactggcgca ccgacgaccg ccgcggtcga ttcaaccgcc
      241 tccgcattga tccggcccgt caagaccatc cctggctccc gacggcccgg tcgtccatcc
      301 attgtcatga gcgatcgttc ctgaaccgaa ccgatcgaca accgcccgac cggtttttgc
      361 cgccgaggga tcgtctgagc caagaacacc cggacgccca cccttcgttc gaccgttgac
      421 cccgtgctgc cgggcgtcaa ggggggcact gaggacggct cgaacgtccc cagcggcatc
      481 agggcgcgcc gcacgcgcct ctcgaatact cttttcccgc gaccaggttc catggacccg
      541 tcgaagtctg atcgccgcca tcgccatccc tggctcgggc gccgaaagag cccatgcgtc
      601 gcggttctat gacgtgacta ggccgagtct tgtacccgag atctaaggat ctcgcaagcc
      661 cggaaggtcc acggatctcc ccggcttgga agctggattt tccgaggtac gagcggccgg
      721 tcgttaccgc caggtccgaa ggccaatgcg caaaagccgc taaggcttgc gcgccagtaa
      781 cccgacgtgc ccggaaccga agcgcaacct gccatagccg cggagaacgc gcactcgaaa
      841 gccgatactc cgcaactgcg ctgccaattc cgacctcggc aatagccgct gacaatgtac
      901 ttcctgatcg cggcggtaaa cctcgccgac ctggcgaaaa ctggtgattc tgcgcgtcaa
      961 gaatttccgc tttcggtcct cttcactgct gactaacact gcccaaccgt ttccttcgaa
     1021 gtacgttcgt gccagtccgt taccgggcac acgccctggc tcggcggcgt caaaaagaaa
     1081 cacgcccccg ggttgcaggg cgcggtggat ccgctctagt aactttgcca gactcgacct
     1141 ggtgttttca gggtcaaaca aatagttgat ggcctcgcca accgccgcga cggccactgc
     1201 gggcgggagc tcggacttga gcagcgattg cacgcgaaac tcgccgcgcg gcacgcgctg
     1261 acgcgccaga gcaatcattg cagcggagat gtcgacgccc aagacgtcgt agccggcctg
     1321 gtagatcgcg cgcgacagga tgccgctgcc gcatccgaga tcgattacga gcccctggtc
     1381 gattccaccg gccttgagcg cctcaaccaa taccggagcc gccgcctgag cgaagccgcc
     1441 gaaacccgca tcgtggatgt gggccaggtc gtctcgatag gaatccgtca ttggtcggtc
     1501 ctgggtggaa atctcccacg gacgggaatc gaacttcgtc agcacggccg cagtttggga
     1561 aatctgcatc gagaccattc gccaagctgc ccgctctcgg cgacggtgcg ccatgatggt
     1621 atcagaatcg acgccgcagg ctcaatttcg tccgcttgtg ccgcttgaat cggttacgtg
     1681 ggcgattggc accttctggc cagtcgaaat aacgtgaata gtctaaatcg cccaggaatg
     1741 agacatgttg gtcagacgcc gaatgggcgt tgtcgaacga tgccagaatc ttttggcgcg
     1801 cagtccgaca cgtcgcgtac cgtaggcagt tccgccctcg aaagccggaa agagagagcg
     1861 gcggccgcaa ctctcgccgg aagagcaggc caggattgcg gcgctatccg ccgacctccc
     1921 agcgctttgg cacgcccccg gcacgaccta ccgggatcgc aaggagatcg tccggcatct
     1981 ggtggacaag gtcatcgttc acgtcgagcg cgacagcgaa tatgtgagcg tcacgatcta
     2041 ctggcaaggt tgcttcacca gccagcacga ggtcgtccgt cccgtcaggt cctacgagca
     2101 gctccgcgac ctcgacaagc tgatggaccg gatcgc
//
LOCUS       DATAQL010001105         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4692561, whole genome shotgun sequence.
ACCESSION   DATAQL010001105 DATAQL010000000
VERSION     DATAQL010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4692561"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VHS97_19095"
     CDS             complement(<1..999)
                     /locus_tag="VHS97_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3450369.1"
                     /translation="MFSRFFIERPIFANVIAIMTMLVGVVTLVGLPIERYPDITPPTV
                     LVSTTYPGADAKVLSDTVASLIEQEVNGVEGMLYMSSTCSSDGSYKLTVTFEVGTDLD
                     KAQVLVQNRLAVAQPRLPVEVQRQGITAKKQSTNIIMAVSLISPNNRYDDLFLSNFAS
                     LRAKDELSRIYGVGEVQVIGSSNYGMRIWLDPEKLKARNLTTEDVLLSIREQNVQVAA
                     GQVGQYPSPKGQGFQFNVTTLGRLSNPDEFGEIILKADDSIFARSEGGVGAARLTRVK
                     DVARVELGSQVYDQWCEIGGKPAATLAVFQLPGANALDVAKKVRATMEKLKPSFPDG"
     gene            complement(1018..2367)
                     /locus_tag="VHS97_19100"
     CDS             complement(1018..2367)
                     /locus_tag="VHS97_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450370.1"
                     /translation="MTVKMSEAAYKALATTMIGLLGLAAGCGETNKYVEPPPPEVTVA
                     RPVTREVTNYVQVTGTAQPLLSVDIRARVKGFLKERLFREGTLVKAGQLLLVIDEEPF
                     RVQHEQAKARLAEAEASLRKTKQSRSREVARAQLALDDSQLQLARLAEARQRNLTARG
                     AGTREEMDQAEASRKKNEAQVEATRASLDQAEADYLTNILGSEANVASARTAVRNAEI
                     ELGYCRMYAPIDGRISRVNLSIGNVVGDGQSSLLATIVKIDSIHAYVNVSEYDLLRYR
                     SYADSPGQPVAGPESMPMELGLANERGYPHRGQGDYQDPGVDPGTGTIRVRGIFPNPD
                     SAILPGMFVRIRVPFERRKDSLLVPERALGTDQSGQYLLVVGQDDRIEYRPVKAGGQL
                     DGLRVVEGKIGPEDRVVVDGLLRARPKLKVNPKPEIVADNAIPVADIGPSLERPSRP"
     gene            2578..3237
                     /locus_tag="VHS97_19105"
     CDS             2578..3237
                     /locus_tag="VHS97_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3450371.1"
                     /translation="MSDKGQRVRDPEGTRTAIIDAAERLIAENGFAATSMRDISNASG
                     VSHPLIHHHFGSKEELYSAVKRRLVEGYAKQFPRAARAVNRPLGVRAEMRRLLAFLGG
                     NPMLLRLCLRTRLEGDNQVWPGEPDQLDTMRRRIEVSQKRGLIRADLDATYLSIMILG
                     LVCFCLENREHFAHRFGEGFDVRSYLRQAIALVERGLAPGPDDPATLDRVESFEKEEL
                     Q"
     gene            3234..3551
                     /locus_tag="VHS97_19110"
     CDS             3234..3551
                     /locus_tag="VHS97_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX3450372.1"
                     /translation="MSVTIHEKESSTLLAQADVGPSLTRYEGNWYFDPSTVQADVLRV
                     TERTYTCPQKGTCNWVDYIGPDGRTVKDVAWVYPQVKPGHEVIQGRYGFYAGSMGATK
                     EEN"
     gene            3592..4476
                     /locus_tag="VHS97_19115"
     CDS             3592..4476
                     /locus_tag="VHS97_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEX3450373.1"
                     /translation="MSETPTDPANAVTPAAISPKTRAMRLRRWTIRGMAALAIVVGIW
                     LILAYVILPMLWRHYEHNPALADAPKTTLTSQRIPGDPLNVGLIGGEEEVVKAMHAAG
                     WSPADPITLKSTVRIATSVVFKRPDPDAPVSGLYLFGRRQDLAFEKAVGGNARHRHHV
                     RFWQWTAADRAGQPLWIGSATFDISVGFSHLTGQVTHHIAADIDAERHSLIEDLIAAG
                     WLSRTYQVTGVGATIAGRNGGGDRYFTDGELTIGVLASNALAGRRPDRLPNPPAVQIK
                     EQLWSAIGPLLQATTPSP"
BASE COUNT          862 a         1538 c         1523 g          893 t
ORIGIN      
        1 cccatcgggg aatgaaggtt tgagcttctc catggtcgcc cggacctttt tggcgacgtc
       61 cagcgcgttg gcgccgggga gctggaagac ggcaagagtc gcggccggct tgccgccgat
      121 ctcgcaccat tggtcgtaga cctgcgaccc cagctcgacg cgggccacgt ccttgacgcg
      181 cgtcaagcgg gccgccccga cgccgccctc tgaccgggcg aagatgctgt catcggcttt
      241 gaggatgatc tcgccgaact cgtcggggtt gctcagccgc cccagcgtcg tgacgttgaa
      301 ctggaagccc tgcccctttg gcgacgggta ctgcccgacc tggccggcgg cgacctgtac
      361 attttgctcg cggatcgaga gcagcacgtc ctcggtcgtc aggttgcggg ccttgagctt
      421 ctctggatcg agccagatcc tcatcccgta gttgctgctg ccgataacct ggacctcgcc
      481 gacgccgtag atccggctca gctcgtcctt ggcccgtagt gacgcaaaat tgctcaagaa
      541 gaggtcgtcg taacggttat tgggcgagat cagcgagacg gccatgatga tgttggtcga
      601 ctgcttcttg gcagtgatcc cctgcctctg gacctccact gggagcctcg gctgcgcaac
      661 ggccaggcgg ttctgcacaa ggacctgggc cttgtcgaga tcggtgccaa cctcgaacgt
      721 cacagtcaac ttgtatgagc cgtcgctcga gcaggtggag gacatgtaga gcatcccctc
      781 cacgccgttg acctcctgct cgatcaagga cgccacggtg tcggagagca ctttggcgtc
      841 cgcgccggga taggtcgttg agaccaggac ggtgggcggc gtgatgtcgg gataccgctc
      901 gatgggcagc ccgactaagg tcacgactcc gacgagcatc gtcatgatcg cgataacgtt
      961 ggcgaagatc gggcgttcga tgaagaagcg ggagaacata aaggtcctcg gatggcctca
     1021 gggacgcgat ggacgctcta ggctcgggcc gatgtcggcg acaggaatgg cgttgtccgc
     1081 gacgatctcg ggcttaggat tgaccttgag cttcgggcgg gcccgcagaa ggccgtcgac
     1141 gaccacgcgg tcctcgggcc cgatcttccc ctcgacaacc cgcagcccgt cgagctgccc
     1201 gccagccttg accgggcgat actcgatgcg gtcgtcttgg cccaccacca ggaggtactg
     1261 tccggactgg tcggtcccca gggcccgctc ggggactaac agcgagtcct tccgccgctc
     1321 gaaggggacg cggatgcgga cgaacatccc cggtaagatc gcgctgtccg ggttcgggaa
     1381 gatgccgcgg acccgaatcg tcccggtccc cggatcgacg cccgggtcct ggtaatcgcc
     1441 ttggccccgg tgcgggtagc cccgctcgtt cgccaggcca agctccatcg gcatcgactc
     1501 cgggccggcg accggctgtc ccggcgagtc tgcgtagctc cggtagcgga gcaggtcata
     1561 ttcgctcacg ttcacgtatg catggatcga gtcgattttg acgattgtcg ccagcagcga
     1621 cgactggccg tcgccgacca cgttgccgat gctcaagttg acgcggctga tccggccgtc
     1681 gattggggcg tacatgcggc aatagcccag ctcgatctcg gcgttgcgca cggcggtccg
     1741 cgccgacgcg acgttggctt cggagccgag gatgttggtc aggtagtcgg cctccgcctg
     1801 atcgagactg gcgcgcgtgg cctcgacctg cgcctcgttt ttcttgcggc tggcttcggc
     1861 ctggtccatc tcctcgcgag tgccggcgcc ccgggccgtc aggttgcgct gccgtgcctc
     1921 ggcgagccgg gccagttgga gctgcgagtc gtcgagcgcg agctgcgccc gggccacctc
     1981 tcgcgaccgt gattgcttgg tcttgcgcag cgacgcctcg gcctcagcca acctcgcctt
     2041 ggcctgctcg tgctgcacgc ggaacggctc ctcgtcgatg accagcagta gttggcccgc
     2101 cttgacgagc gtcccctcgc ggaacagccg ctccttgagg aagcccttga cccgtgcccg
     2161 gatgtccacc gagaggagcg gctgggccgt cccggtgacc tgaacgtaat tggtcacctc
     2221 ccgcgtgacg ggcctggcca cggttacctc cggcggaggc ggctcgacgt atttgttcgt
     2281 ctcaccgcag ccggccgcca ggccgaggag cccgatcatg gtggtggcca gcgctttgta
     2341 agccgcctcg gacatcttca ccgtcatcac cactcccctc caacgccgtc ccctgaccga
     2401 tcggtcagca atacttaccg tacggtcaat atatttcggg cgcggttacc gcaaagtcaa
     2461 gctcaaagtc ctccagaatc tccgcgaccg cgattttccc ggcggcgcag cgattgacgg
     2521 aggagtcgat ggttcctatc atatttacca gtcggtcagc acgggcagga gggggcgatg
     2581 tcggacaaag gccagcgcgt cagggaccct gaaggcacgc ggacggccat catcgacgca
     2641 gccgagcgct tgattgcaga gaacggcttc gccgcgacgt cgatgcgcga catttcaaac
     2701 gcctcaggcg tctcgcatcc gcttatccac caccatttcg gaagcaagga agagctctac
     2761 agcgccgtca agcgcaggct ggttgagggg tacgcgaaac aattccccag ggccgcccgg
     2821 gcggtcaacc ggccactcgg cgtcagggcc gagatgcgcc ggttactcgc gttcctgggc
     2881 ggcaatccaa tgctcctgcg tctttgcctg cggactcggc tcgaggggga caaccaggtc
     2941 tggcccggtg agcctgatca gctcgacaca atgcgccgac ggatcgaggt ctcgcagaaa
     3001 cgggggctta tacgcgccga tctggacgcg acctacctga gcatcatgat cctcggcctc
     3061 gtttgttttt gtctagagaa ccgcgagcac ttcgcccatc gctttggcga ggggttcgac
     3121 gtacggtctt acctccgcca ggctatcgcc ctggtcgagc ggggtctggc cccaggcccc
     3181 gacgatccgg caaccctaga tcgagtcgaa tcgttcgaga aggaggaact ccaatgagtg
     3241 tgaccatcca cgagaaagag tcgagcacgc tactggcgca agccgacgtg gggcccagcc
     3301 tgacccggta tgagggcaac tggtacttcg atccgtcgac agtccaggcc gacgtcttgc
     3361 gggtcaccga gcgcacctat acgtgcccgc aaaagggaac ctgcaactgg gtcgactata
     3421 tcgggcccga cggccggacg gtgaaggacg tggcgtgggt ctatccccag gtcaagcccg
     3481 gtcacgaggt gatccagggt cggtatgggt tctacgccgg cagcatgggt gcgaccaagg
     3541 aagagaattg agcgacatcg tcaggtgttg atcaattgcg agcggccgtg catgagcgag
     3601 actccgaccg atcccgccaa tgcggtcacg ccagcggcga tttccccgaa aacacgcgcc
     3661 atgaggctgc ggcgctggac gatccggggc atggcggcct tagccatcgt cgttggtatt
     3721 tggctcatcc tggcctacgt catactaccg atgctctggc gccattatga gcataatccg
     3781 gcgctcgccg acgcgcccaa aaccacgttg acgtcgcaga ggatccccgg cgatccgctc
     3841 aatgtagggt tgatcggcgg cgaggaagag gttgtcaagg cgatgcacgc tgcgggttgg
     3901 agccctgccg acccgatcac cttgaagagt actgttcgca tcgcgacgag cgtcgtattc
     3961 aagcggccgg accctgatgc acccgtgagc ggcctttatc tctttggccg caggcaggat
     4021 ctggcgttcg agaaggccgt cggcggcaac gcgcggcaca ggcaccacgt gcggttctgg
     4081 caatggaccg ctgccgatcg ggcggggcaa ccgctctgga tcggatcggc cacttttgac
     4141 atcagcgtgg ggtttagcca cttgaccggc caggtgaccc atcacatcgc ggcggacatc
     4201 gacgccgaac gccacagcct gatcgaagac ttgatcgccg ccggctggct ctcgcgaacc
     4261 taccaggtga caggcgtcgg agcgacgatc gccggacgca acggaggagg ggaccggtat
     4321 tttaccgacg gcgagctgac tatcggcgtc ctggccagca acgctctcgc cggccgccgc
     4381 cccgaccggc tgcccaatcc cccggccgtc caaatcaaag agcaattgtg gtcggcgatc
     4441 ggcccactgc tgcaggcgac aacaccgagc ccctgagctt gtgttgtgtc gagcgagtct
     4501 atcatggcat gctctcatta atgcgcatgc ccatgcacac tccatgcttt agcccaccga
     4561 tgagttgcgt ccacccgcaa gcggtaaaaa cggcgcattc tttgcacgct cattacccga
     4621 tcgccccgtg aatttccagc gtactcaaag gaggaggtgt gaacttatgt cagcaacgac
     4681 aaccaatcaa gctcacattg cttacgaact gatcaacgac acggatcctg gggtcgtggt
     4741 aatcgagttt cgcagccacg aggtcgccag tttcttgcac tcacgggagc tgggagaaca
     4801 gcttcaatcg ctaatc
//
LOCUS       DATAQL010001106         2897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4385727, whole genome shotgun sequence.
ACCESSION   DATAQL010001106 DATAQL010000000
VERSION     DATAQL010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4385727"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..2046)
                     /locus_tag="VHS97_19120"
     CDS             complement(367..2046)
                     /locus_tag="VHS97_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450374.1"
                     /translation="MGKVYRHPAMKQLKDQQTRYAPRERRLEQVERAEQLLGEIEGAK
                     HYPYEYLCFRITGFRPDGWPALLLEGGDVQHDLRMFVEDLSATARQAVEQVEEPVLTV
                     DEVSRRYNVSSRTVARWRRQGLVARRFVIDGRTKVGFLESSLQRFVTAHRTQVERGTR
                     FRQLTEAERDEIIRRARRMALVRPGQAGLVEIARRIGRKMSRSTETIRLTLKAHDRDH
                     PDRAIFPNTLPPLDDEAKAQIYRRFRLGVSAEVLAKQYGRTRSSVYRVINEMRARRIL
                     GTKLELIEHSSFALREMAAEILGPIPEPGEGKASRRPKAPKGLPPYLASLYEVPLLDR
                     EQEMHLFRKMNYLKYQSNLIRSNVDPTRAKTADLDQIDRLQEEALAVKNQIIRANLRL
                     VVSIAKRHVGPSNNFFELVSDGNMSLIRAVEKFDFSRGNKFSTYASWAIMKNFARTIP
                     EENYRRDRFVTGHEEMFEAAADNRTDEHEYESALKRMQEAVKGMLGRLDDRERKIIIS
                     RFGLGGASEQTLEQLGRELGITKERVRQIESRAQDKLRKIAGEEKLDLPML"
BASE COUNT          581 a          879 c          779 g          658 t
ORIGIN      
        1 cggtgggttc gtcggcccgg gcttctcaga tcgcgacgcg attcgcccgc tgttgtcgcg
       61 gcccacggac ctcaaacagc caaggtcgaa acgacggccg ccatggtcga tagcgcattg
      121 cgctcgatgc ggttcgccac aaacatgaac gcgggctctc cgagccgccg cgctcgatcc
      181 gcaattcgtc ccagagcgta ccgggaccaa attcctgggt cgctcgaaag ggctcgaacg
      241 gctcgaccga ttctttatag gtgcgaccct gtcgtactag ggcccgcacc acgctgaagt
      301 tggctgtaaa ggcgccgggg atgcacctgg gtctccagat cgggcatcct ttccgaggcg
      361 tttcgatcag agcatcggca agtcgagctt ttcctcaccg gcgatcttgc ggagcttgtc
      421 ctgagcccgc gattcgatct gacggacgcg ctccttggta atccccagtt cgcgaccgag
      481 ctgctccaag gtctgctcgc tggcgccccc aaggccgaag cggctgatga tgatcttgcg
      541 ctcgcggtcg tccaaacggc cgagcattcc cttgactgcc tcctgcatgc gcttcaaggc
      601 gctttcgtac tcgtgctcgt cggtccgatt gtcggcggcg gcctcgaaca tttcttcgtg
      661 gcccgtgacg aaccggtccc gtcggtaatt ttcctcgggg atcgtccgag cgaaattctt
      721 catgatggcc cacgaggcgt acgtgctgaa cttgttgccg cgggaaaagt caaacttttc
      781 gacggcccgt atcagcgaca tgttgccatc ggagaccagc tcgaaaaagt tgttcgaggg
      841 tccaacgtgc cgtttagcaa tggacaccac caaccggaga ttggcccgga tgatctgatt
      901 cttgaccgcc agagcctctt cttgcagccg atcgatctga tcgaggtccg cggtcttggc
      961 tcgggtcgga tcgacatttg aacgaatgag attcgactga tatttgagat aattcatctt
     1021 gcggaacagg tgcatctcct gctcgcgatc cagcaagggg acctcgtaca ggctcgcgag
     1081 gtacggaggc agacccttgg gcgccttggg ccgtcgcgat gccttgccct cgcccggctc
     1141 gggtataggt ccgaggattt cggcagccat ttcgcgaagt gcaaagctgg agtgctcgat
     1201 cagctcgagc ttggtgccca ggatgcgcct ggctcgcatc tcattgatga cgcggtagac
     1261 gctcgatcgc gtccgcccgt actgtttggc aaggacttcg gccgaaacgc ccaggcgaaa
     1321 gcggcggtag atctgcgcct tggcttcatc gtccagcggg ggcaacgtat tgggaaagat
     1381 cgcccggtcg gggtgatcgc ggtcatgtgc cttcaaagtc agtcggattg tttctgtaga
     1441 gcgtgacatc tttcgtccta tcctccgcgc gatctcgacc aggccggcct gccctgggcg
     1501 aactagcgcc atgcggcggg cgcgacggat aatctcgtct cgctccgcct cggtcagttg
     1561 ccgaaaccgt gttcctcgtt ccacctgcgt gcgatgggcc gttacaaatc gctgcaggct
     1621 cgactcgagg aacccgacct tggtgcgccc atcaatgacg aagcggcgcg ccaccaaccc
     1681 ttgccgccgc cagcgtgcca ccgtgcgtga cgacacgttg taacgccgac ttacttcatc
     1741 cacggtcaag accggctctt caacctgctc gacagcctga cgcgctgtcg cagacaagtc
     1801 ttcgacgaac atccgcagat catgctgaac gtctccacct tcgaggagga gggcaggcca
     1861 accatcgggc cgaaatccag tgattcggaa acaaagatat tcgtaagggt agtgcttcgc
     1921 tccttcgatc tcgcccagaa gttgctcggc ccgttccacc tgctcaagac gccgttctcg
     1981 cggcgcgtac cgagtctgtt gatctttgag ctgtttcatt gcgggatggc ggtacacctt
     2041 acccatcatc gtttttcccc ccgctccacg aggtccgcca agctcccggg cgacgtcact
     2101 ctgtgtcgct cgtaccaacg acgtccgcgc ttgatgactc aacgatcgaa cttcctctct
     2161 taatggcgac gaagaaaatc ggacttatcg ccgtggtcat ctgcacgcgg cattccgctt
     2221 ttcaaggaat tcgttattgc aactcgattt attataattc gttcagtcaa aaaccacgtg
     2281 agccaaaaca tgtcttttgg tcggcccttt tccgtttcgc tttgatctca gtgtctctaa
     2341 tacgtattat ttgcaccgct ggatcgtttc attttgtgac gagcgcggac aggcctcctc
     2401 acacaatgac gcgcgtccca tgattgaggt tctgtttact cagcacgaat cacattccca
     2461 cgcatccgtc cccacgccat cagcacagtt tcgaagcagc ccgatgcaaa actcgtgcca
     2521 gaacctcgat gctgcgatga ctcgaaacta ttcatatcaa cgtcaaagtc gcagcaaagt
     2581 gaaccgcgca acgcaacaaa aaatccaacg ctcgtttcaa acagacgttt gaatcaagat
     2641 ttttgttcgc tcaattcgct gcaggcgccg catatcgctg gcgcttaacg cgtaagccgc
     2701 tattagacaa caggatcgag acaaccattc aactcggccg cgttggcgga gttatcaaag
     2761 atttctccgc tttttcggat gcgcattgcc tttcggagca tgcattgatt tggagagtcg
     2821 gcgaacatcc atccatgata tcacaaaaac ttggtcgccg caatgccaaa ttgacagctt
     2881 cctgcaagta tcaataa
//
LOCUS       DATAQL010001107         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4846179, whole genome shotgun sequence.
ACCESSION   DATAQL010001107 DATAQL010000000
VERSION     DATAQL010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4846179"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VHS97_19125"
     CDS             complement(<1..1257)
                     /locus_tag="VHS97_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450375.1"
                     /translation="MDRWTTTRRTFLETLTAGGVATSALASLELPRGYGANDTLTVGC
                     LGTGGRCQALMKSLAQVPGVKIAAVCDVYDAHLEKGKRLADAQAVATKHYREVLDRKD
                     IDAVLIASPDHWHVPMAVDACEAGKDVYVEKPLTHDLAEGAAIVEAQNRHHKIVQVGM
                     QQRSMPHIQKGRELVKSGRIGELYKIRLSWNRGGSKRMERKPQGVDPRQVTWKDFVGR
                     AADQPFDEYRFRNWRWFWDFGGGLLTDLMVHWIDVAHWFLDVDHPEKATAIGDHFASN
                     GLWQTPDTIQCLLEYPNNVQAHFEGTFSNANLGAMITFMGTEGTLYIDRGRYELTPEY
                     GKGQPESWILGSNPQKGADFYDKPDGELLHLTNWVDCIRTRQRPNAPAEAGVSAASAA
                     HLGNRAYRSGQVATWTSAGEGDSVRSA"
     gene            complement(1358..2698)
                     /locus_tag="VHS97_19130"
     CDS             complement(1358..2698)
                     /locus_tag="VHS97_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX3450376.1"
                     /translation="MRDPLVRGVDLMQSIYWDLALILGLILASGLVAMSEFAISLARK
                     SRLRDLASRGYRGAAVALRLSEDPKGLLWTVHGSMTLLGTLTGVYAGANLTPKLVHAL
                     AQAVPLARYCQMIAVGLIVATISLLSIVLAELVPRRIALIRPERIARLISGPVRALAT
                     VAVPLFRLLSKATDRGLRVLGIRPRPDPPVTQEEISILLREGTKAGVFEEGEHEMIKR
                     VFRFSDRRARALMTPRNEVVWIDITDPTDEIRRKVKNSPHTRFPVCDQSLDNLLGIVQ
                     VKDLLVQDQNVDAFRLKGRLTLPLFLYEGTRGLKILEMFKKSGSRVAVVLDEYGTVEG
                     MLTLTDILEAIVGDMPDGDEDEDPPVVQRADGSWRLDGMMPLDEFRELFELPSLPEGD
                     FHTLAGLVVTELGHIPRVAESFDSWGLHFEIAEMDGNRVNRILVWRLADVGKHS"
BASE COUNT          561 a          927 c          873 g          582 t
ORIGIN      
        1 tgcggagcgg accgaatcgc cctctcctgc cgacgtccag gtcgcgacct ggccagagcg
       61 atacgcgcga ttgccgagat gggccgccga ggcggcgctc acacctgctt cggccggagc
      121 attggggcgc tgccgagtcc ggatacaatc gacccagttg gtcaagtgca agagctcgcc
      181 atcgggtttg tcgtagaaat ctgccccctt ctgggggttg ctccccagga tccacgactc
      241 cggttgtccc ttgccgtact cgggcgtgag ctcataccgg ccccgatcaa tgtacaaggt
      301 cccctcggtc cccatgaacg tgatcatcgc acccaggttt gcgttcgaaa aggttccctc
      361 gaaatgagcc tgcacgttgt tggggtattc gagcaagcac tggatcgtgt cgggggtctg
      421 ccacagtccg ttgctggcaa aatggtcgcc gatagcagtg gccttctcgg gatggtccac
      481 gtcgagaaac cagtgtgcga catcgatcca atggaccatc aggtccgtca ggagaccgcc
      541 accaaaatcc caaaaccagc gccagtttcg aaagcgatat tcgtcaaagg gctggtcggc
      601 ggcacgtccg acgaagtctt tccaggttac ctggcgagga tcgacaccct gcggtttgcg
      661 ctccattcgt ttcgagccgc ctcgattcca tgagaggcgg atcttatata gctcgccgat
      721 ccgcccgctc ttgacgagct cacgtccctt ctgaatgtgg ggcatgctgc gctgctgcat
      781 tccgacttgc acgatcttat ggtggcgatt ttgtgcctcg acgatcgccg ccccctcggc
      841 aagatcgtgc gtgagcggtt tttcaacgta aacgtccttg ccggcttcgc acgcatcgac
      901 cgccatcggt acgtgccaat ggtcggggct ggcgatcaag accgcatcga tgtctttgcg
      961 gtcgaggact tcgcgatagt gcttggtggc caccgcctgt gcatccgcca gccgcttccc
     1021 tttttcgaga tgggcgtcgt agacatcgca caccgccgcg atcttgaccc cgggcacttg
     1081 ggccagagac ttcatcaggg cctgacagcg cccgcctgtt cccaggcagc cgaccgtgag
     1141 tgtgtcgttg gccccataac ctcgcggcaa ttcgagcgac gccaatgcgc tggttgcgac
     1201 tccaccagca gtgagggtct cgaggaacgt acgccgcgtc gtcgtccagc gatccatggc
     1261 tttactcctc atcgcaggca gaacgtgtcg atctgctgga agtctagccg tcgccacgat
     1321 cacgggcaac caaccagcgg tgattccagc ctacgggtca ggaatgtttg ccgacgtcgg
     1381 ccagccgcca gaccaggatg cgattgacgc gattgccgtc catctcggcg atctcgaagt
     1441 gcaagcccca gctatcaaag ctctcggcta ctcgaggaat atggcccagc tccgtgacga
     1501 ccagtccggc cagagtgtgg aaatcgcctt cgggaagcga tggcagctcg aaaagctcgc
     1561 gaaactcatc cagaggcatc atcccgtcta agcgccaaga cccatccgcg cgctggacga
     1621 ctggcggatc ctcgtcttca tcgccgtcgg gcatgtcccc cacgatggct tccagtatgt
     1681 ccgtgagcgt cagcataccc tcgacggtac cgtattcatc gagcacgacc gcgacgcgcg
     1741 atcccgattt tttgaacatc tccagaatct tgagcccgcg cgtaccctcg tagagaaaaa
     1801 gcgggagggt caggcgcccc ttgagccgga atgcgtcgac attctggtcc tggaccaaaa
     1861 ggtctttgac ctggacgata cccagcaggt tgtcgagact ctggtcgcag accggaaagc
     1921 gggtgtgcgg gctgttcttg accttgcggc ggatctcgtc ggtcggatcg gtaatgtcga
     1981 tccatacgac ctcattgcga ggcgtcatca gcgcacgagc acggcggtcg ctgaagcgga
     2041 agacgcgctt gatcatctcg tgctcgccct cctcgaaaac gcccgccttg gtcccctcgc
     2101 ggagcaaaat tgagatttct tcctgggtga cgggtggatc cggccggggg cggattccta
     2161 ggacgcgcag tccgcgatcg gtcgccttgc tcaacaggcg aaagagtggc acagccacgg
     2221 tcgcaagcgc cctgaccgga cccgatatca gccgggcgat ccgttccggt cgaatcaaag
     2281 caattcgtct gggcacgagc tcggcgagca cgatcgacag caggctgatc gttgcaacga
     2341 ttaaaccgac cgcgatcatc tggcaatatc gcgccagggg gacggcttgc gccagtgcgt
     2401 gcaccaattt gggcgtcagg ttggctccgg cgtaaacgcc cgtcaaggtt cccaagagcg
     2461 tcatcgaccc gtggaccgtc cagagcaggc ccttggggtc ctcgctcaag cggagcgcga
     2521 ccgccgcgcc gcggtatccc cggctggcca gatcacgcaa ccgcgatttc ctagccaagc
     2581 tgatggcgaa ttccgacatc gcgaccaaac cgctggcaag gatcaatccc aggatcagcg
     2641 ccaagtccca gtaaatcgat tgcatcagat cgaccccgcg gaccaacgga tcgcgcaaga
     2701 gacacgctct cgcgattgat ccgacaaacc atcatgttag ctgcgcgccg ggccgtttgc
     2761 tgcctggtac catcgcgcgt tcgccgcttt tgacacggac ggcaaattca ggtgccatgg
     2821 gcgttgggtg cggtgtcagc tgcgtttatc cgcctgtcgg gtaatcgcgg cgccctttca
     2881 cttgggcgtg cgtacaggca gagcaaatag cgtactccgg tgggatcccc tgggtcgatc
     2941 tcg
//
LOCUS       DATAQL010001108         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2405863, whole genome shotgun sequence.
ACCESSION   DATAQL010001108 DATAQL010000000
VERSION     DATAQL010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405863"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VHS97_19135"
     CDS             <1..207
                     /locus_tag="VHS97_19135"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3450377.1"
                     /translation="QFVDAQTAPLFHRLITRFRELTGVPMVLNTSFNDREPLVATPDD
                     ALKTFSQTHIDALFLGDRLVRQTG"
     gene            188..1219
                     /locus_tag="VHS97_19140"
     CDS             188..1219
                     /locus_tag="VHS97_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450378.1"
                     /translation="MYDKRDKVKEPELNIEGTFAAAMPSVAPREASLFEPMRDRYGWR
                     LALVFGNHAQGGRCPYFPRSCFHCDIGAGEGTAFDHTASRQRLDWFRDHYRDTLDLIN
                     HLVLYNSGSILNPREMPPELLDEILSFARSLAALRIVSVDSREAYIRPATLMRMVAIL
                     GPGIMLCPILGMETADDRLRDDVLQKAMPRAAIDRVYRDLKALDLGLEPGRIGLAVNV
                     VIASPGTSPDTAAEDAVRTARYALDLGSHYGIKVDLNLHPYYRGDRGGARFPDHPRCP
                     LATTVRAVSEIAALVRSMAADTSIFIGWQDEGHDQDRPQRDGDLARSRAAFERFNRTN
                     DPDTLYGLW"
     gene            complement(1229..2470)
                     /locus_tag="VHS97_19145"
     CDS             complement(1229..2470)
                     /locus_tag="VHS97_19145"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3450379.1"
                     /translation="MRLATRLSSWVVLLSATSLGAAAQDDGAKSAAKPDATPGAQAQA
                     SAARRRQATPNDTLKSAEVAPDHKVTFRIYAPKADEVSVSGDFGAGAKLTKDEQGVWS
                     ITVGPLTPDFYSYTFNVDGVRTVDPKNPMIKPGIASLDNMLLVPGDEAEFESTKDVPH
                     GEIRTAWYRSSTLDSLRSMHIYTPPGYDSGTEKYPVFYLLHGGGDDDSGWPTIGRAGF
                     IVDNLLAEKKAVPMIVVMPNGSLPRPANMPRFTPGSTPSPEARAAMETLQSRFTNELL
                     KEIIPFTEAHYRVRTGPENRALAGLSMGGGQTLRVITTNPDQFSQIAIWSAGLFGGNP
                     DEFEKRNESFFKAADHVNKTVKLLSISVGDKDFTLDGAKGLAGLFEKNGIKHELHISG
                     GGHTWINWRHYLNELAPRLFR"
     gene            complement(2687..>2940)
                     /locus_tag="VHS97_19150"
     CDS             complement(2687..>2940)
                     /locus_tag="VHS97_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450380.1"
                     /translation="ETTLLVSRNAVATDFTRFIGFIESGRLDTTPWNTHRCPATPVPE
                     EFSRRLDSVQCDVNVMKRSHWQPMRVLRASELSKNPDET"
BASE COUNT          526 a          892 c          895 g          627 t
ORIGIN      
        1 cagtttgtcg atgcccagac tgccccgtta ttccacaggt tgattacgag gttccgcgag
       61 ctcacgggcg tgccaatggt gctcaacacg tcgttcaatg accgggaacc gctggtcgcc
      121 acgcctgacg atgccctcaa gacgttctcg caaactcata tcgatgccct gttcctcgga
      181 gatcgccttg tacgacaaac gggataaggt aaaagagccg gaacttaata ttgaagggac
      241 gttcgccgcc gcgatgccca gcgtggcgcc gcgcgaagcc agcctcttcg agccaatgcg
      301 cgaccgctat ggttggcggc tggcactcgt tttcggcaac catgcccagg gcgggcgctg
      361 cccttatttt cctcgctcgt gttttcattg cgacatcggt gccggcgagg ggacggcatt
      421 tgatcacacc gccagccgcc agcgactcga ttggtttcgc gaccactatc gcgacacact
      481 tgatttgata aaccacctcg tactttacaa ttccggctcg attctcaatc ctcgcgagat
      541 gccgccagag ctactggacg agatcctcag ctttgcccgc tcgctcgccg cacttcggat
      601 tgtctccgtc gattcgcgtg aggcttatat caggccggca actctcatgc gaatggtcgc
      661 aattcttggt cctgggatca tgttatgccc gattctgggc atggaaacgg ccgatgaccg
      721 gctgcgcgac gacgtccttc agaaggccat gccgcgcgcc gcaatcgacc gggtttatcg
      781 cgacttgaaa gcactcgacc tcggccttga gcccggaagg atcgggctcg cagtgaacgt
      841 cgtgattgcc agcccgggca cgtcccccga cacggcggcc gaagatgctg tgaggacggc
      901 caggtatgcg ttggatttgg gcagtcatta tggaatcaag gttgatctga atctccaccc
      961 gtactatcga ggcgaccgcg gcggagcccg gtttccggat catccgcgtt gtccgctggc
     1021 gacaaccgta cgagcggtct ccgaaatcgc cgccctggtt cgatcgatgg cggcagacac
     1081 gtcgatcttc atcggctggc aagacgaggg ccacgaccaa gatcggccgc agcgcgacgg
     1141 cgatctcgcg cgcagtcgcg ctgcgtttga gcgattcaat cggacgaatg atcccgacac
     1201 gctctatggc ctgtggtaat cgctcttgtc atcgaaacaa tcgcggcgcc aattcattca
     1261 aatagtgtct ccagttgatc caggtgtggc cgccgccgct gatgtgcagc tcgtgtttga
     1321 tgccgttctt ttcgaacagg cccgcgaggc ctttcgcgcc gtccagcgtg aagtctttgt
     1381 cgccgacact gatcgacaat agcttgacgg tcttgttgac atgatcggcg gctttgaaga
     1441 aggattcgtt ccgtttctca aattcgtcgg gatttccgcc gaacaggccg gcgctccaga
     1501 tcgcgatctg actgaactgg tcagggttcg tcgtgattac gcgcaaagtc tggccgcctc
     1561 ccatcgatag accggcgagt gcccgattct cgggcccggt ccgcacgcga taatgcgcct
     1621 cggtaaaggg gatgatctct ttgagcagct cgttggtgaa ccggctctgc aaggtctcca
     1681 tggcggccct cgcctcaggc gaaggggtgg atcccggcgt gaaccgaggc atgttggcgg
     1741 ggcgaggcag gctgccgttg ggcatcacca cgatcatggg cacggccttt ttctcggcca
     1801 gcaggttgtc gacgatgaag ccggcgcggc cgatcgttgg ccatcccgaa tcgtcatcac
     1861 ctccaccgtg taacagatag aagaccgggt atttctcggt gccgctgtcg tagccaggcg
     1921 gggtatagat gtgcatactc cgcaaggaat caagggtgct ggaacgatac caagcggtgc
     1981 gaatctcgcc atgcgggacg tctttggtcg actcgaattc ggcctcgtcg ccgggtacca
     2041 gcagcatgtt gtccaggctg gcgattcctg gtttgatcat tgggtttttg gggtcgaccg
     2101 tgcgaacgcc gtcaacattg aacgtgtagc tgtaaaagtc aggtgtgagc ggaccgaccg
     2161 taatcgacca gacgccctgc tcgtccttgg tcagtttggc ccctgccccg aagtcgcctg
     2221 agaccgagac ctcgtccgcc ttgggagcgt agatgcgaaa ggtgaccttg tggtcgggcg
     2281 cgacctcggc cgatttgagc gtatcgttgg gcgtggcctg tcggcggcga gccgccgagg
     2341 cctgagcctg cgctcccggc gtggcatccg gcttcgcggc cgacttggcc ccatcgtctt
     2401 gagcggccgc cccgagggaa gttgccgaca agagaacaac ccaactgctc aagcgcgttg
     2461 ccaatctcat gcctcgctcc tccgcgtgtg ctgtgttttt cgcctcaatc gtcgccgcaa
     2521 ccgccagaaa gctactctcc gccgctcgcc cgggaaatcg agctcgcctc gcacgcgtcg
     2581 gcggctccga cttctttcgg accgcgttca gtatcggacc gacggagggc tcggtcaacg
     2641 ggtccgtgtc cccgcttccg ccggaccgac gatcggtcgc atatggctac gtttcgtcgg
     2701 ggtttttgga aagctcggac gctcgcagaa cccgcatggg ttgccagtga cttcgtttca
     2761 tcacgttcac atcgcactgg accgagtcga gccgtcgcga gaactcctca ggcaccggtg
     2821 tggccgggca gcggtgtgta ttccaggggg tggtgtcgag tcggccggat tcgataaacc
     2881 cgatgaaccg cgtgaagtcg gtagcgaccg cgttgcggga cacgagcagc gtggtctcac
//
LOCUS       DATAQL010001109         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1487816, whole genome shotgun sequence.
ACCESSION   DATAQL010001109 DATAQL010000000
VERSION     DATAQL010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487816"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            653..2794
                     /locus_tag="VHS97_19155"
     CDS             653..2794
                     /locus_tag="VHS97_19155"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3450381.1"
                     /translation="MSARENIRPLPAAMPQPAGMPSAIPFVRRARPGLITDWHLGLLA
                     IIYIRQSTPQQILDHRESRERQYALVNYAVAMGWPRDRVLIIDDDQGLSGTTAENRGG
                     FHRILAQVTMEHVGLILGIEMSRLARCNEDWHHLLQICSLFGTVLADEDGLYDPRDPN
                     DRLLLGLKGTMSEYELITMHNRLEGGKLHKAQRCELILTVPCGYLKLPTGEVVLDPDE
                     QARSTVRLVFDKFHELGSFGRLYRYLVRNKIRLGMRVQRGAERGQLEWRPPTLGTVAR
                     ILHHPIYAGAYSYGRRRVDHKRAASNGSKARIREVPMSQWTVLQTDRLPAYITWDHYL
                     ANQERLRQNRYQPGSVGAPRAGKALLTGILVCSACGRRMSASYRSKSTAYYMCTQMRS
                     DGSNCVGLASSAIDDLITKQVLRALEPAALELSLKAIENVQQERQRLNRHWEQRLERA
                     SYDVQRAERQYQAVEPENRLVARSLERQWEAALRAQRDLKEEYDRFVNEQPPRLTEEE
                     RSHIRAVSADIPTLWHASETTARDRKEIIRLLTERIVVDVRSNTERAEVTITWRGGQT
                     TRHEIVRSVSRYESLGEYDRMMDRIVQLRRDGLTIKALAAQLTREGYRTPRTQKGYTP
                     TSVRQLLSRRGLTGGAIGREQLESGEWWLPDLAQKLGTSYDRLRDWALHGKVRARRVQ
                     PGGPWIVWANSQERRRLRKLVANAEQNNASA"
BASE COUNT          650 a          867 c          910 g          520 t
ORIGIN      
        1 gcaaccggta cagcaaactc caggatgatc cgtcggccat gcgtctgcgg ccgaacgggg
       61 acgaaaagca ggcgttccaa ggtatcgcca agcgaaggaa acgaagtgcg gcgaaaagac
      121 tgtcaggaag tcggagtgcc ccatagtagc gtcgaagcgg gggaacgggc cctaccggac
      181 cccggggagc gaagggggtg ccgcgttgtg gaccagacgc cggaaccacg ctgaggacac
      241 cgtgcctcac cagcgtgtca ccgcaagccg atggatcgtg tgagggacag cgccacaacg
      301 tgacgagcca gatgcgttta acgcgcacgt ctggatgtgg ggagcaaatt gctcgtctag
      361 tcacacctgg tcagataccg ccaacccagc ctatcatcgg gttgccggcc ctgctccgac
      421 tggtggagtg gtaccaggag cgccggcgga acgcccagca aggagggttg ccatgcgtaa
      481 atctcgaacc gccgttgtcc ccccccgcgc cgcagcaagg taactcgtca gccgattcct
      541 cagcggctgt gggtgacctt tcccgtcgag caacaacgac gggttctcaa cgcgctgacc
      601 cgcgtcgtcg cccaacaact ggcgaagcct ccggacgtga aggaggtgac gcatgagcgc
      661 tcgtgagaac attcgcccac ttcctgcggc aatgccgcaa ccagcgggca tgcccagtgc
      721 aatccccttc gtccgtcgcg cacggcctgg cttgatcacg gattggcacc tcggtttgct
      781 ggcgattatc tatatccgcc agtccactcc ccagcaaatc ctcgatcacc gagaatcgcg
      841 tgaacgacag tacgcgctgg tcaactacgc ggtcgcgatg ggatggcccc gtgaccgtgt
      901 cctaatcatc gacgacgacc aagggctcag cggcaccacg gccgaaaacc gtggcggatt
      961 tcaccgcatc ctcgcccaag ttaccatgga gcacgtcggc ctgatcctcg ggatcgagat
     1021 gagtcgattg gcccgctgca acgaagactg gcatcatcta ctgcaaatat gctcgctctt
     1081 cggcactgtc ctggcagacg aggacggcct ctacgaccca cgagatccca acgaccgtct
     1141 tctcttgggg ttgaagggga ccatgagtga atatgagcta atcacaatgc acaaccgcct
     1201 ggaagggggc aagcttcaca aggcccaacg gtgcgagttg atcctaaccg tgccatgcgg
     1261 gtacctcaag ctccccactg gcgaagtcgt tctcgatccc gatgaacagg caagatcgac
     1321 cgtccgtttg gttttcgaca agttccatga acttggttcg ttcgggcggc tctaccgcta
     1381 cctggtacga aacaagatcc ggttggggat gcgggtgcag cgaggcgcgg agcggggcca
     1441 actggaatgg cgcccgccca cgctgggaac ggtggctcga atacttcatc acccgatcta
     1501 tgccggcgct tattcttatg ggcgtcgccg cgtcgatcac aaacgagcgg cttcaaatgg
     1561 aagcaaggcc aggattcgcg aggtgccgat gtcgcaatgg acggtgctcc aaacagatcg
     1621 actgccagct tatatcacct gggaccatta cctggccaac caagaacggc tgcgtcagaa
     1681 tcgctaccag cccggctctg tcggtgcgcc tcgtgccggc aaggcgctgt tgaccggcat
     1741 cttggtttgc agtgcctgtg gtcgacggat gagcgcgtcc taccgaagca agtcaaccgc
     1801 ctactatatg tgcacgcaaa tgcggagcga tggctccaat tgcgtcgggc tggcgtcgtc
     1861 ggccattgac gacctgataa caaaacaggt ccttcgagcc ctggagccgg cagccctgga
     1921 gttgagcttg aaggcgatcg agaacgtgca gcaggaacgc cagcgcctca accggcactg
     1981 ggagcaacgg ctcgagcgcg cttcgtatga tgtccagcgt gccgaacgcc agtaccaggc
     2041 cgtcgagccc gaaaaccgct tggtggcgcg gagcttggag cgacagtggg aggcggcgct
     2101 gcgcgcacag cgcgatttga aagaagaata tgatcgattt gtaaatgaac aacctcctcg
     2161 gctcacggag gaagaacgca gccacatccg agcagtctcg gccgacattc cgacactctg
     2221 gcacgcgtct gagacaaccg cgagggatcg caaggagatc atacggcttc tcaccgagcg
     2281 cattgtggtc gatgttcgct ccaacaccga acgtgccgaa gtaacgatca cgtggcgagg
     2341 tggccagacc acgcgtcacg agatcgtcag gtctgtctcc cggtacgagt ctctgggtga
     2401 atacgatcgg atgatggaca ggatcgtgca actgcgtcgc gacggcttga cgatcaaggc
     2461 gttggcggcg caactcactc gagaggggta tcgtacgccg agaactcaaa agggctacac
     2521 gccgacttcg gtgcgccagc tcttgtctcg ccgcgggcta accggaggcg cgatcggccg
     2581 agaacagctc gagagcggcg aatggtggtt gcctgacctc gctcaaaagc tcgggacttc
     2641 atacgacagg cttcgcgact gggcgctaca cgggaaagtt cgtgcaagga gagttcagcc
     2701 gggagggccg tggattgttt gggcgaacag ccaggaacga cgaaggttgc gaaagctcgt
     2761 ggcaaacgcg gagcaaaata acgcatcggc gtaggccgtt aaagtaataa agctattatg
     2821 ttgacaacta ttcatcaaac tggcccgccc cgaccgttct aagcggtcgg ggccttgtga
     2881 caatagaggt attatgccgc gcaaatctgg atctgtgggg ggcctgggtg ggagaccacc
     2941 caggtct
//
LOCUS       DATAQL010001110         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3930941, whole genome shotgun sequence.
ACCESSION   DATAQL010001110 DATAQL010000000
VERSION     DATAQL010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3930941"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1066
                     /locus_tag="VHS97_19160"
     CDS             35..1066
                     /locus_tag="VHS97_19160"
                     /inference="COORDINATES: protein motif:HMM:NF036733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450382.1"
                     /translation="MKEPVADTGEIANPRGLLISKNFAVDPADNVTGYKNFHAGSFDF
                     KRDEYFACITTPKGFHTIPVDAFLRALMRDVAWGFFYGTVNFDQVFGTTNYYGEVDMF
                     LGATNESYTSAGRDLIERFKSNELMDIFKQMVSDWTIEGYDPFAAPMETGTPWGVKNG
                     DNDPAVGRKRVTARRMVGLKGDTPIRTDQNGYPVNRMFDDVKQSAPEVYPEPGFENEV
                     SAFNLFAYLSRSDVTWNPSVCSVVKDSLFCPTSEEYILPVEHGNDRCEWFVTLSDEIV
                     WNIKDGATGRPKAVVRQRAGDCACMPADIRHQGFSAKRAMLLVWENGSPKVPDLIREG
                     KAPTVAVSF"
     gene            1150..>2588
                     /locus_tag="VHS97_19165"
     CDS             1150..>2588
                     /locus_tag="VHS97_19165"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3450383.1"
                     /translation="MQTRLFINGDFVDAHARTTISVVNPYDNSLICEIAEARAEDVDA
                     AVDAAKAAQKGWARTDPSQRGRLLLKLADALEEEAEEFIRIESMDTGHPVRDARRLDV
                     PRTSACFRYFGVIADKFEGFLPPVYPGYLNYVTREPVGVVGQIVPWNFPLMFTSWKMA
                     PALAGGNTIVIKPSELTPLSTLKIAELMKRVGFPDGVVNVVPGYGHTAGARIAEHPDI
                     KKIAFTGSTATGRRIVQASASNLKKVQLELGGKGPNIIFSDADIRASVNGSAWAIFHN
                     QGQACIAGSRLILHEAVADEFLDRFLTLARSIRLGNPLDAETEMGPLTSRQHQERVLH
                     YVKVARQEGGEVLAGGRIPDREDLAKGCFVEPTVVRARPTDTVSQEEVFGPFVTVTTF
                     SDDDEALAIANGVKYGLGSGLWTSNLSRAHRIAREINAGMVWINSYKVVHPGSPFGGV
                     GESGYGREMGFEAMREYTQPKSVWVNVDAP"
BASE COUNT          509 a          766 c          815 g          498 t
ORIGIN      
        1 ctgaaatgaa acgggaggac ccttgctcat ggccatgaaa gaaccagtcg cggataccgg
       61 cgaaatcgct aatccccgag gattgttgat ctcgaagaat tttgctgtcg atccggccga
      121 taacgtaacc ggttacaaga atttccacgc cggttccttc gatttcaagc gcgacgaata
      181 tttcgcctgc attacgaccc ccaaaggctt tcatacgatc cccgtcgatg ccttcctgcg
      241 ggcgctaatg cgggacgtcg cgtgggggtt cttttacggc acagtgaatt tcgatcaggt
      301 attcggaacg accaactact acggcgaagt ggatatgttc ctcggcgcga ccaatgagtc
      361 ctacacatcg gcgggtcgcg atctcatcga gcgattcaaa tccaacgagt tgatggatat
      421 tttcaagcag atggtgtccg actggacgat cgagggatac gacccgttcg cggcccctat
      481 ggagaccgga acgccgtggg gcgtgaagaa cggcgacaat gacccggcgg tcggccgaaa
      541 gcgggtgaca gcgcgtcgca tggtcggcct gaaaggcgac accccgatcc gcaccgacca
      601 gaacggatat ccggtcaacc gcatgttcga cgacgtgaag cagagcgcgc ctgaagtcta
      661 tccggagccc ggcttcgaaa acgaggtgtc ggcgttcaac ctgttcgcct acctttcgcg
      721 gtcagacgtg acgtggaatc cgtcggtttg ctcggtcgtc aaagacagcc tgttctgccc
      781 gacgtctgag gaatacatcc ttccggtcga acacggcaat gaccgctgcg agtggttcgt
      841 cacgctgtcg gacgagatcg tatggaacat caaggacggc gcgaccggca gaccgaaagc
      901 ggtcgtcagg cagcgggctg gcgattgcgc ttgcatgccg gccgacattc gccatcaggg
      961 cttttcggcc aagcgggcga tgctgctcgt ctgggagaac ggctcgccga aggttcccga
     1021 tctcattcgc gagggtaagg cgccaaccgt ggccgtgagc ttctaggaat tacgccagac
     1081 gcgaaaagtc gccggctgcg cgcgtccccg cccgctggag aaattgtcgc ggggctgagg
     1141 acctcggata tgcagacgag acttttcatc aacggagact tcgtcgacgc gcacgcccgc
     1201 acgacgatct cggtggtgaa cccctacgac aattctctca tatgcgaaat tgcggaggct
     1261 cgggcggaag acgtcgacgc ggcggtcgat gcggccaagg cggcgcaaaa gggctgggcg
     1321 cggacggacc cttctcagcg cggccggctt ctcctcaagc ttgctgatgc gctcgaggaa
     1381 gaggcggagg aattcattcg catcgagtcg atggacacgg gccaccccgt gcgtgacgcg
     1441 cgccggctcg acgttccccg cacctccgcc tgctttcgct attttggcgt gattgcggac
     1501 aagttcgagg gttttctccc accggtctac cccggttacc tcaattatgt gacccgcgag
     1561 ccagtcggcg tggtcggcca gatcgtgccg tggaacttcc cgttgatgtt cacgagctgg
     1621 aagatggcgc cggcgctggc gggaggcaac acgatcgtca tcaaaccttc ggagctcacg
     1681 cctctgtcga cgctgaagat cgccgagttg atgaagcgag tgggttttcc ggacggggtc
     1741 gtgaacgtgg tccccgggta tggccacacg gcgggggcgc gcatcgccga acacccggac
     1801 atcaagaaga tcgcctttac cggatcgacg gcgacggggc gtcgcattgt ccaggcgtcg
     1861 gcctcgaacc tcaagaaggt tcaactggag ctcggcggca agggacccaa catcattttt
     1921 tccgacgccg acattcgcgc ctcggtcaat ggctccgcct gggcgatctt tcacaaccag
     1981 ggccaggctt gcatcgcggg gtcccggctt atcctgcatg aggccgtcgc cgacgaattc
     2041 ctcgatcgct tccttacgct cgctcgatca atccggcttg gaaacccgct cgacgccgag
     2101 acggagatgg gacccctcac ctccaggcag caccaggagc gcgtcctgca ttacgtcaag
     2161 gtcgcgagac aggaaggcgg agaggtcctt gctggcggcc gcatcccgga tcgcgaggac
     2221 cttgcgaagg gctgtttcgt cgagccgacg gtcgttcggg caaggccgac cgatacggtc
     2281 agccaggagg aggttttcgg gcccttcgtc acggtgacga ccttctcgga cgacgatgag
     2341 gcgctggcga tcgccaacgg cgttaaatat ggacttggct ccggcctgtg gacttcgaat
     2401 ctgtctcgcg cccataggat cgcgcgcgag atcaatgccg ggatggtctg gatcaactcc
     2461 tataaggtgg tccatcccgg ttcacctttc ggcggcgttg gagaatccgg gtatggccgc
     2521 gagatggggt tcgaggcgat gcgtgaatac acccagccga agtcggtctg ggtcaatgtc
     2581 gacgcgcc
//
LOCUS       DATAQL010001111         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2710665, whole genome shotgun sequence.
ACCESSION   DATAQL010001111 DATAQL010000000
VERSION     DATAQL010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710665"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            600..2375
                     /locus_tag="VHS97_19170"
     CDS             600..2375
                     /locus_tag="VHS97_19170"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX3450384.1"
                     /translation="MRWSHALIPTLKETPADAVAPSHVLLLRAGMIRQLGAGAYTYMP
                     LGLRVIQKVIEIVRQEMDAAGGIELLMPALQPVELWKESGRYQAYGDLLMKLTTSSGH
                     QMALGPTHEEVITDLVRDLLRSYKQLPITLYQIQTKFRDEPRPRFGIVRTREFIMKDA
                     YSIDADIDQLNQSYDAMYEAYCRIFDRCGVPYVIVEAESGPIGGDSSHEFMVPCATGE
                     DKVIQCVACGYAANQERAEIGGQKEAAIERPADAPPYQAVTTPNKRTIRDVCEFLKVD
                     ETTSAKLLVYLADGKPVAALVRGDHELNEPKLRRVIGATALAPADPATIEKATGTPMG
                     FLGPVAIKIPLVIDRAIAAMPTVVVGGNAVDVHLKGVVPGRDFPLAQVHDLRNADAGD
                     PCPRCGQPLKITAGLEIGHVFKLGTKYSKSMGASYLDDKGNEVLHIMGCYGIGINRIV
                     AGAVEAAHDPNGILLPLALAPYHVVVVPLQVQNAAVLERAEQLEKALAAAGLDVLIDD
                     RDQRPGVKFKDADLIGIPLRVVVGERGLKDGTIEIKWRHQPEARHIPAETAAESILAE
                     VTAARRRHDAYCEERRQARAAGRGE"
     gene            2372..>2576
                     /locus_tag="VHS97_19175"
     CDS             2372..>2576
                     /locus_tag="VHS97_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450385.1"
                     /translation="MTKHPLPRLPYFLLGGLTCASFGGPFVILMVVRGGTSARWPPDR
                     PVEWAAIALVFGLVVALFLACVSI"
BASE COUNT          480 a          771 c          854 g          471 t
ORIGIN      
        1 ccaaatcgtg gtaacgacaa cgccgccggg ctggcgggga gtagcgcgag tggcggaaaa
       61 caggagcgtg cccaggacga cgtgggcagt ggggtgaatg acctgaacga gccggcggat
      121 gcggacgagg gaaacccgca aggcgtcgac ccgtcatttc cacgcgccac ggtgccgccg
      181 tgggctgtca gccgacttgc cgaactgctt gtgcctatgg actcagccgc actcgctctt
      241 cttagcgatg cggagcgccg agcgcggagt tgggcgcagt ttcacgcgaa acgaaagttc
      301 cagagcggtt cgtagtacgt gctaaaccca cggccgctgg ccgtgatcga ttattcattg
      361 gtcattggtc gttagttagt tggcagccaa attgttcgaa ggtctcgagc gcggatcacg
      421 tttggtagga gcgattgtat ttcgaagcga tttgtcggct ccgccctggc gactcgactg
      481 cgacacgtgc attgaatgtg ttaagttaga ggtttccccg tcgagcggcc aatccgatcg
      541 aagtgcggtt cagcccggtt cgatgaattc ttgttgtccg tagcaggagg tttgatctgg
      601 tgcgctggtc gcatgccctg atcccaacgt tgaaggaaac accggccgac gccgtggcgc
      661 ccagtcatgt gctgctcttg cgcgcgggca tgatccgcca actgggcgcg ggggcttaca
      721 cttacatgcc cctggggctt cgcgtcatcc agaaggtgat cgagatcgtc cgccaggaga
      781 tggatgccgc gggagggatc gagctgttga tgcccgctct ccagccggtc gagctctgga
      841 aagagtcggg acgataccag gcgtacggtg atttgctgat gaagctaacc accagcagcg
      901 gccaccagat ggcgcttggt cccacgcacg aggaagtcat caccgacctc gtacgcgacc
      961 tgctccggtc gtataaacag ctgccgatca cgctctacca gatccagacc aagttccgtg
     1021 acgagccgcg cccgcggttc ggaatcgtcc ggactcgcga attcatcatg aaagatgctt
     1081 acagcatcga cgccgacatt gaccagctca atcagagcta tgacgcgatg tacgaggcgt
     1141 actgccggat ctttgaccgg tgtggggtgc cctacgtgat cgtcgaggcg gagagcgggc
     1201 cgatcggcgg tgactcgtcc cacgagttca tggtcccgtg cgcgaccggc gaagacaagg
     1261 ttattcagtg cgtcgcgtgc gggtatgcgg ccaaccagga acgggccgag atcggtggac
     1321 aaaaagaggc cgccatcgag cgacccgcgg acgccccccc ctaccaggcc gtcacaacgc
     1381 ccaacaaacg gaccatccgc gacgtctgcg agtttctcaa agtggacgag acgacatcgg
     1441 caaagcttct cgtctacctg gccgacggca agcccgtggc ggcgctcgtg cgcggggacc
     1501 acgagctcaa cgagcccaag ttgcgccggg tgatcggggc gacggcactg gcacctgccg
     1561 acccggcgac gatcgagaag gcgacgggta ctccgatggg ttttctgggc ccggtggcga
     1621 tcaagatccc actggtgata gaccgggcga tcgccgcgat gccgacggtc gtcgtcggtg
     1681 gcaacgccgt ggatgtgcac ttgaaggggg tcgtgccggg gcgcgacttt cctctggcgc
     1741 aggtgcatga cctgcggaat gccgatgccg gtgatccgtg cccccggtgc ggacagccac
     1801 tcaagattac cgcgggattg gagatcggcc acgttttcaa gctcggcacc aagtactcca
     1861 agtcgatggg ggcaagctac ctcgacgaca agggaaacga ggttctccac atcatgggtt
     1921 gctacggtat cggcatcaac cggatcgtag ccggcgccgt cgaggcggcc cacgacccca
     1981 acggtatcct cttgccgctg gctctcgcgc cttatcatgt ggtcgtcgtg ccgctccaag
     2041 tccagaacgc cgcggtgctc gagcgggcag aacagctcga gaaggcgctg gcggcggccg
     2101 ggctggacgt cttgattgac gaccgcgatc agaggccggg cgtgaaattt aaggacgccg
     2161 acctgatcgg gatcccgctc cgggtcgtgg tcggcgagcg cggtctgaag gacggcacga
     2221 tcgagatcaa gtggcgtcat cagccagagg ccaggcatat cccagccgaa acggcagccg
     2281 agtcgatcct cgcggaggtc accgcggcac gccggcggca cgacgcgtat tgcgaagagc
     2341 gccggcaggc gcgcgccgcc gggagggggg aatgacgaaa cacccactcc cccgtctgcc
     2401 ctattttctg ctgggcggac tgacgtgcgc atcattcggc ggcccgttcg tcattcttat
     2461 ggtcgtccgc ggcggtacca gcgcccggtg gccgccggac cgtccggtcg agtgggcggc
     2521 catcgcgctt gtgttcggat tagtcgttgc gctttttctc gcctgtgttt ccattg
//
LOCUS       DATAQL010001112         4743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2861511, whole genome shotgun sequence.
ACCESSION   DATAQL010001112 DATAQL010000000
VERSION     DATAQL010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2861511"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..370
                     /locus_tag="VHS97_19180"
     CDS             224..370
                     /locus_tag="VHS97_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450386.1"
                     /translation="MSSAADQIKAIRDKVKAGQRLSFDDGMALEASNDLFALGSMANL
                     MRER"
     gene            360..1379
                     /gene="mqnE"
                     /locus_tag="VHS97_19185"
     CDS             360..1379
                     /gene="mqnE"
                     /locus_tag="VHS97_19185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEX3450387.1"
                     /translation="MSGNGNFTYFNVNTHINPTNVCVYTCDFCAFRADLGEDRAYVMD
                     RDQIVERARQASARGATELHIVGGLHHKLPLGYYLDVVRWIRETAPEIHIKAYTAVEI
                     EWFCKISRKSVQQVLKELIDAGLGSLPGGGAEIFHPEVREQICGAKASTESWLNVHRT
                     AHRLGLHSNATMLYGHIDGPNHRIDHLIRLRELQDETGGFQTFIPLAFHPDNSEMAHI
                     PKPSGVMDLKVMAISRLMLDNFPHIKAYWVMLGLKTAQVALSFGADDLDGTVVHEKIY
                     HEAGAETPQEATVAEIRRLITEAGRIPVERDTLYHRIERESGSWASREHIDVPALAVL
                     NAVRN"
     gene            1415..2263
                     /locus_tag="VHS97_19190"
     CDS             1415..2263
                     /locus_tag="VHS97_19190"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3450388.1"
                     /translation="MSVAPSKPVPAVPNSPDHLPGHLELPEENGEFVQNSRELPQSLL
                     LSSAIWPILENIHPDRHFAVGQDSGIYWRLTNPPERGAVAPDWFYVPGVPPDLDGHYR
                     RSYVLWKEHISPAVIIEYASGDGTEERDRSPLTGKFWIYEQAIHGGYYAIIVVETGEL
                     EVYRLEGTRYRRLEPNKHGNYLIEPLGVELGIWHGFFWNETAPWLRWYDKHGSLLPIA
                     DERANEEYRRAEEAIRRAEDERSRAEEERFRADSQQRRADQLAAKLRELGVDPGEIES
                     GFMPGA"
     gene            2433..3155
                     /locus_tag="VHS97_19195"
     CDS             2433..3155
                     /locus_tag="VHS97_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3450389.1"
                     /translation="MREHNKAFCSLVAETFECPGPVFEFGSYQVEGQEGYANLRGLFA
                     AKSYVGCDMRPGPGVDRVEDVTAISLPDGSAGTVLCIETFEHVFAVSKAFDEVFRLLR
                     PGGMFVITSPLNFRIHGYPDDYWRMTPNCLRRHLEPYAARLTGFQGYHAFPHTVMGVG
                     IKAPAPLDCAGRLERLAAAYRTWLGRTEASLPFGEKFRRRMSMFYRSKGERHQLAAYY
                     EADFNVDLIRVVEPATRGRVSA"
     gene            3235..>4743
                     /locus_tag="VHS97_19200"
     CDS             3235..>4743
                     /locus_tag="VHS97_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3450390.1"
                     /translation="MPKTVDRLVVLGLDGATWSVLDPMRQRGLMPNLQAFLARAASGT
                     LRSIVPPVTTAAWTSMMTGCGPARHGVFDHRYYDAAEARMKVNHSGRIRVPTLWHLLS
                     STGRSIVSLNVPGLYPPLKVPGIVVSGMDAPHLEAALSAAPEFAARREAEAPGYGLGY
                     FWKRTPQSLDELTNNARLTAASFLGRAQGGWLADKIVPDWSVMMVQFQNLDPFQHRVW
                     PYLNVDDTGIDRPEWNDAAAQVIVGLDDAIGHLCELAEKRRAGVMMVSDHGFGPCFGR
                     IHVNRILIKAGVARLPGWPGLIERRTLQVGDRLRLWGQKRDDPAARSASFDQSVTAQY
                     PFDWKRTLAFAPHQDTAAMVYVNCAARHGGVKTSAPRMTPRQIDDARSAAAMALAEAK
                     HPEYGSPLFPQIIETAAAYQIDPTREGYPDLIALPDEPYWVRTKLTSSVSWVEPDPNL
                     PGTHRPEGIVALGAAGLAPGRYLNAHLIDATPTILALLGQAIPAHIEGKPIVG"
BASE COUNT          965 a         1465 c         1400 g          913 t
ORIGIN      
        1 tctcgccgcc ggtcatcatt gaatacgcgt ccgggtccag ttcgggatag gcggcacgtc
       61 gcgttcccgg caattcggcg atcgactccg gttgatgatc cgttcaatcg cgactaaaat
      121 tactgaaaga ggtcatcgag cctgagtccc gacctggcgg ttgtaaaagg cgatgatcca
      181 ttggggttgc atggcgttcc gagcgtgaga ggacggacga acgatgtcga gcgcagcaga
      241 ccaaatcaag gcgattcgcg ataaagtcaa ggccggccaa aggttgagct ttgatgacgg
      301 catggcactc gaagcgtcga acgacctctt cgccttgggc tcgatggcga acttgatgcg
      361 tgagcggtaa cggcaacttc acgtacttca acgtcaacac ccacatcaac ccaaccaacg
      421 tctgcgttta cacttgcgac ttttgcgcat ttcgagccga cctgggcgaa gaccgggcct
      481 atgtgatgga tcgcgaccag atcgtcgagc gggcgcggca ggcgagcgct cgcggcgcta
      541 ccgagttgca cattgtcgga ggcctgcacc acaagttgcc gttgggctat tacctcgatg
      601 tcgtgcgctg gatcagggag acggctcccg agatccacat caaagcgtat acggctgtcg
      661 aaatcgaatg gttctgcaag atctcgcgca aatccgtcca acaggtgctc aaagagctca
      721 tcgacgccgg cctcggcagt ctgcccggtg gaggtgcgga gatcttccac cccgaagtcc
      781 gtgagcagat ctgcggcgcc aaggcctcaa cggagagctg gctcaacgta caccggacgg
      841 cccaccggct gggcctgcac tccaatgcaa ccatgctcta cggccacatc gacggaccca
      901 accatcggat cgaccatctc atccgcctgc gcgaactcca ggacgagacc ggcggctttc
      961 agacgtttat cccgctggcg ttccatcccg acaactccga gatggcccat attcccaagc
     1021 cctcgggtgt gatggatctg aaggtcatgg cgattagtcg actgatgctc gacaactttc
     1081 cccacatcaa agcctactgg gtgatgctcg ggctcaaaac cgctcaggtt gctctttcct
     1141 tcggagccga tgatctcgat gggacggtcg tccacgaaaa aatctatcac gaggcaggcg
     1201 cggagacacc ccaagaagcg accgtcgccg aaatcaggcg cctgattacc gaggctggac
     1261 ggattccggt ggaacgcgat actctctatc accgtatcga gcgcgaaagc ggatcttggg
     1321 cctcgcgcga acacattgac gtgccggcct tggccgtgct caacgctgtc agaaactaga
     1381 atctccggct ctcctccatc acggaggcgg ttgcatgtcg gtcgcacctt ccaagcctgt
     1441 cccagcagtc ccaaactcgc ccgatcacct tccaggccat ctggaattgc ccgaagaaaa
     1501 tggcgaattc gtgcagaatt cccgcgagct gccccagagc ctcttgctgt ctagcgccat
     1561 ctggcccatc ctcgagaaca ttcatccgga taggcatttc gcggtggggc aggattcggg
     1621 aatctactgg cgtctcacca acccgccgga aagaggcgct gttgcccctg attggtttta
     1681 cgtgcccggc gttcctcctg atctcgatgg acactatcgg cggtcgtacg tgctgtggaa
     1741 ggaacacatt tcaccggcag tcattattga gtacgcatcc ggcgacggca cggaagagcg
     1801 cgatcgctcg ccacttacag ggaagttctg gatctacgaa caagcgatcc acggaggcta
     1861 ctacgccatc attgtcgtgg aaaccggtga actcgaggtc tatcgactcg aagggaccag
     1921 ataccgacgg ctcgaaccca acaaacacgg gaactacctg attgagccgt tgggagtcga
     1981 actagggata tggcacggct ttttctggaa cgaaacagct ccgtggcttc gttggtacga
     2041 caaacacggc agtttgcttc ctatcgccga tgaaagagca aacgaagaat atcggcgagc
     2101 cgaggaagca attcgcagag ccgaagatga gcgttccagg gccgaggaag agcgtttcag
     2161 agccgacagc cagcaacgcc gcgccgatca gctcgcggcc aagctgcgcg aactgggcgt
     2221 cgatccgggg gaaatcgaat ccggcttcat gcctggtgcc tgatcggccg cacatcgggc
     2281 gaacccgaat cgcgttcgac ttgaccccat cgtcggctgg gttatgatcg ccccgatcaa
     2341 gtgcgtggga aaggcgatcc ggcgcgaaac cgaactgtac cgcgacgtct tcaccctccc
     2401 acacccgtcc ggctcaagga ggagcagccc cgatgcgcga gcacaacaag gcgttctgca
     2461 gcctggtcgc cgagacgttc gagtgtcccg gccctgtttt tgaattcggc tcataccagg
     2521 tcgaaggcca agagggctac gccaatcttc gtgggttgtt tgccgccaag agctacgttg
     2581 gctgcgatat gcggcccggt cctggtgtag accgtgtgga agacgtcacg gcaatcagcc
     2641 tgccggacgg atctgccgga acggtgcttt gcatcgaaac attcgagcat gtattcgccg
     2701 tcagtaaggc attcgacgag gtctttcgcc tgctcaggcc cggaggcatg tttgtcatca
     2761 catctccgct caacttccgg attcacggct acccggacga ttactggcgg atgacaccca
     2821 actgcctacg acgccatttg gagccatatg cggcgcggtt aaccggcttt cagggctatc
     2881 atgcctttcc tcatacagta atgggggtcg ggataaaggc cccggcaccg ctcgactgcg
     2941 cgggcaggct cgagcggctc gcagcggcgt atcgcacctg gctcgggcga accgaagcca
     3001 gtctgccgtt cggcgaaaaa tttcgccggc gaatgtcgat gttttatcgc tccaagggcg
     3061 agcggcatca gctcgccgcc tattacgagg cggacttcaa cgttgacctc atccgcgtgg
     3121 tcgagccggc gactcgcggg cgcgtatccg cgtagcgccc tattggggcg gttcgctgag
     3181 cggtcatgac gcgagtctgg tgacgctcag ccagataaga ttaggaacga ttccatgccg
     3241 aagaccgtcg accgactggt tgtgctgggc ctggatggcg cgacctggag cgtgttggac
     3301 ccgatgcgac agcgggggtt gatgcccaac ctccaggcgt tcttggcccg cgccgcctcg
     3361 ggcactctgc gttcgatcgt accaccggtg acaacggcgg cgtggacgtc gatgatgact
     3421 ggctgcgggc cagcacggca cggcgttttc gatcatcgct actacgacgc agccgaagcg
     3481 agaatgaaag tcaatcactc ggggcggatc cgcgttccga cgctctggca cttgctctcg
     3541 tccaccggcc ggtcgatagt ttcgctcaac gtgcccgggc tgtatccccc gctgaaggtg
     3601 ccgggaattg tggtctcggg catggatgca ccccatctcg aggccgccct gtcggcggcc
     3661 ccagagttcg ccgccagacg cgaggccgag gcgccaggct acggactcgg ctacttctgg
     3721 aaaaggacgc cccagtcgct cgacgagctt accaataacg cccgtttaac cgccgcaagt
     3781 ttccttggcc gcgctcaggg aggctggctc gctgacaaaa tcgttccgga ctggtccgtg
     3841 atgatggtcc agtttcaaaa cctcgacccg ttccagcatc gggtctggcc ctatctgaac
     3901 gtcgatgaca ccggtatcga ccgccccgaa tggaacgacg ccgccgccca agtgatcgtt
     3961 ggtctggatg acgcgatcgg gcacctctgc gaactcgcgg aaaaacgacg tgccggggta
     4021 atgatggtga gcgaccatgg ctttggaccc tgctttggcc gcattcacgt caatcgcatc
     4081 ttgatcaaag ccggggtcgc tcggctgcca ggttggcccg gcttgatcga acgccgaacc
     4141 ttgcaagtcg gtgaccggct ccggctctgg ggacagaaac gcgacgatcc ggccgctcgt
     4201 tccgcgtcgt tcgatcaatc ggtgaccgcc cagtatccgt ttgactggaa acgtacgctc
     4261 gccttcgcgc cgcaccaaga caccgccgcg atggtttatg ttaattgcgc cgcgcgtcat
     4321 ggcggcgtca aaacgagcgc gccgcgcatg actcccaggc agatcgacga cgctcgctcc
     4381 gccgccgcca tggcactcgc cgaggccaag catcccgaat acggctcgcc cctgtttcca
     4441 caaatcatcg agaccgccgc tgcttaccaa atcgatccga cccgggaagg atatccagac
     4501 ttgatcgcgc tgccggatga accgtactgg gtccgcacga aactgacatc gagtgtgtcc
     4561 tgggtcgagc cggaccccaa tttgcccgga actcaccgtc ccgaaggaat cgtcgcgctg
     4621 ggcgcggctg gactggctcc aggccgttat ttgaacgccc atttgattga cgccacgccg
     4681 acaatcctcg ctcttctggg gcaagctatc ccggcgcata tcgaaggcaa gccgatcgtc
     4741 ggc
//
LOCUS       DATAQL010001113         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3471107, whole genome shotgun sequence.
ACCESSION   DATAQL010001113 DATAQL010000000
VERSION     DATAQL010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471107"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VHS97_19205"
     CDS             complement(<1..808)
                     /locus_tag="VHS97_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HEX3450391.1"
                     /translation="MSMVTPRQPKEGLKIVEDATCTFCGCLCDDIALEVEGNRIKEAR
                     NACTLGQTWFYEDRREPDAICLIDGAPAALEDGIDRAARILAEAKYPLVHGLCHTTTQ
                     AQRVAVSIADLIGGCVDTSSNAAHGASTLALQEIGEVTCTLGEVKNRGDLIIFWGCDP
                     AESHPRHLSRYSLEPRGSFVPGGRTDRFCVVVDIRDTATAKLADQFVAITPRTEFEAL
                     WTLRALLKGVELDPAQIEVETGVPLFAWQGLLDRMKRARYGVIFFEPSSQN"
     gene            complement(889..2139)
                     /locus_tag="VHS97_19210"
     CDS             complement(889..2139)
                     /locus_tag="VHS97_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3450392.1"
                     /translation="MGIALRRLKRLAAFFQTGWSILGITLLMLVSVEGGFRLTFGIRD
                     RLTAQAVPDRRVLAEGYGGAAWPVEHYRELVLLQDRWEPYVYFRQKPFHGKTIEIGDD
                     GLRATWQSPASANERGQRGPVKILMLGGSSLWGFGARDNQTIPSLLARSLDERGWRTE
                     IKNLSEIGYVSTQELIALVRELQAGYRPDVVIFYDGVNDTTSAFLEVEAGVTTNEVNR
                     RAEFNLRQSPARLAAALAGKLIQDSGSYRFAQMVRRRLGGGIEPARPRPEGAGIGTLA
                     EQVVRRYEANVGLAASLANSFGFCPLFFWQPTIFSKPKLVSVERDEAHRFKWAEEMFR
                     QVHARIGSSSQLKADATFRDLSEIFAESEGLVFIDYCHITELANARIASLMADGVIQA
                     FERATPSGRNRVPQGGGPAPQGVN"
     gene            2194..>2454
                     /locus_tag="VHS97_19215"
     CDS             2194..>2454
                     /locus_tag="VHS97_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3450393.1"
                     /translation="MNLRDHPGVGPARRNQVFAAMSNNRSGMELQGTRAAVLGSTSGI
                     GRAVALALAHAGADVIVHGRTSRDQAEAVAALVRARGGRSAVL"
BASE COUNT          472 a          751 c          767 g          464 t
ORIGIN      
        1 tgttttgcga gctcggctcg aaaaagatta cgccgtaacg ggcccgtttc atccgatcga
       61 gcaaaccctg ccaggcgaac aggggaactc cagtctccac ttcgatctgc gcgggatcga
      121 gctcgacgcc cttgagcagg gcacgcaacg tccagagcgc ctcgaattcg gtcctgggtg
      181 tgatcgccac gaactggtcg gccagcttgg cggttgccgt atcacgaatg tcgaccacca
      241 cacaaaatcg gtccgtgcgg cccccgggga caaagctgcc gcgcggttcc agactgtatc
      301 tcgaaagatg gcgcggatgg ctttcggccg ggtcgcaccc ccagaagatg atcagatcac
      361 cgcgattctt gacttctccg agcgtgcagg taacctcccc gatttcctgg agcgcaagcg
      421 tggaagcgcc atgagcagca ttactcgacg tatcgacgca accgccgatc aaatcggcga
      481 tcgatacagc gacacgctgc gcttgagtcg ttgtgtggca gaggccatga acgagcggat
      541 acttggcctc cgccagaatc cgtgctgcgc ggtcgatgcc atcctcgagc gctgctggtg
      601 cgccgtcgat caaacagatg gcgtccggtt cgcgtcggtc ctcgtagaac cacgtttggc
      661 ccaaggtgca cgcgttccgg gcttctttaa tgcgattgcc ttcgacctcg agtgcgatgt
      721 cgtcgcacag gcaaccgcag aaggtgcatg tcgcatcctc gacgatcttc agcccttctt
      781 tgggttgtcg cggggtcacc atgctcatgg gtcatgcaac tcaagggaaa agccacggca
      841 taggatcgct catttacaag acagttgggg aggatcgaca gcgcccagtc aatttactcc
      901 ctggggcgcc ggaccgccac cttgagggac ccgatttcgc ccgctgggag tcgctcgctc
      961 gaatgcctgg atcacgccat cggccatcag agacgcgatc cgcgcgttgg ccagctcggt
     1021 gatgtggcag tagtcgatga agaccaatcc ttcggactcg gcgaagatct cgctcagatc
     1081 acgaaatgtg gcgtcggctt tgagctgcga cgaagatccg atcctggcgt gaacctgtcg
     1141 gaacatctcc tcagcccact taaatctgtg ggcttcgtcg cgctcgacgg acaccagttt
     1201 cggtttgcta aagatcgtcg gttgccagaa gaagaggggg caaaaaccaa aagaattggc
     1261 gagactcgcg gcgagcccga cattcgcctc gtagcggcga acgacttgtt cggcaagtgt
     1321 gccaattccc gctccctccg gccgaggacg cgctggttcg attcctcctc caagccggcg
     1381 ccggaccatt tgtgcaaacc gataggagcc ggagtcctgg atcagcttgc cggccaacgc
     1441 ggcggccagt cgtgctggtg attgccgcag gttgaactcg gcgcggcggt tgacctcgtt
     1501 ggtcgtcacg ccggcctcca cctccaggaa agccgaggtc gtgtcgttga ccccgtcgta
     1561 gaagatgaca acgtcaggcc ggtaaccggc ctggagctcg cgaacgaggg caatcagctc
     1621 ctgcgtgctg acgtatccga tttccgacag gtttttgatc tcggtgcgcc atccacgttc
     1681 gtcgagcgag cgagcgagaa gcgatgggat cgtctggttg tcgcgggcgc caaatcccca
     1741 aagcgacgag ccgccgagca tgagaatctt caccggcccg cgctgacccc gctcgttcgc
     1801 tgatgcggga gattgccagg tggcgcgcag accgtcgtcg ccaatctcga tcgtcttgcc
     1861 gtggaacggc ttttggcgga agtagacata gggctcccag cggtcttgga ggagcaccag
     1921 ctcgcgataa tgctcgaccg gccaggccgc tccgccatag ccttcggcga gaacgcggcg
     1981 atcgggtacg gcctgagccg tcagtcgatc tcggataccg aaagtgaggc ggaatccgcc
     2041 ttccaccgac acgagcatga gcaaggtgat acccaggatc gaccagcctg tctggaagaa
     2101 ggccgccagc cgcttcagtc gtcgtagtgc gatgcccatg ggcggactcg acctgggacc
     2161 gtagaatatt gctgggattt cgcggacgtc aggatgaatc tacgcgacca tccgggcgtt
     2221 ggaccagccc gaaggaacca ggttttcgcc gccatgtcca acaatcggtc aggaatggag
     2281 ctgcagggaa ctcgggcggc ggtcctgggt tcaacgtccg ggataggccg cgccgtcgcc
     2341 ttggccttgg cccacgccgg ggccgacgtg atcgtgcatg gacgcacttc gcgagatcag
     2401 gccgaggcag tcgcggccct tgtgcgcgca cgcggcggcc gctcggcggt gctc
//
LOCUS       DATAQL010001114         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4235631, whole genome shotgun sequence.
ACCESSION   DATAQL010001114 DATAQL010000000
VERSION     DATAQL010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4235631"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VHS97_19220"
     CDS             complement(<1..1084)
                     /locus_tag="VHS97_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450394.1"
                     /translation="MTTKQFYKVKRRHSPGAWIPRAVAIAIVLFANSVFPLARSVPAL
                     ETEALAASAEVVFQVSFGPELSKTPLDGRLLVILSTDDKEEPRFQINQSPKTQQIFGI
                     DVDGLEPEQDAVINATAVGYPIESPRHLPPGKYRIQALLHRYETFHRAIGPVVKLPMD
                     RGEGQQWNKAPGNLYSTPREITIEAGPSSGGAIRVRLDKVIPPITDPQTTRYIKHERI
                     ESDKLTKFWGRPMHLGAHVLLPEGFDSHPDARYPLVIFHGHFPQTFGGFREEPPDPNL
                     KPDYSERFHVSGYNRTVQEQAHQFYKEWTGPNYPRMIIIEIQHANPFYDDSYAVNSAN
                     LGPYGDAITYELIPYLEKKYRCIGAGW"
     gene            1592..2269
                     /locus_tag="VHS97_19225"
     CDS             1592..2269
                     /locus_tag="VHS97_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450395.1"
                     /translation="MKRYGLVTVALGWLCLSASGCATPMVARYIYQDADFGVVGIPVN
                     NYQKKTDFRGQAEGLMSRHFPEGYEIVRAEEVNEGERILDLGKKTQIETDPKVAALNQ
                     MISLGSLNRTTSYEQKDKLQLRECRIIYRKKPLHTPGRSGQFASTNSAAPPLYIDPNE
                     ILRHQIKTTGDMLGKANTPSKKPHDGPTPGDAEVKKATVEPTSLNSNLSIPDQLDSIF
                     DQMIKCP"
     gene            complement(2376..3302)
                     /locus_tag="VHS97_19230"
     CDS             complement(2376..3302)
                     /locus_tag="VHS97_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450396.1"
                     /translation="MNCFEPRLHRRRYYRILVILVALSTSTMARGAEPVRLTHDGTLK
                     LCPVFIGGADQVAFASHEGPNLVSIVCLNLSDGSRHRLHPSVVNHQFDPAFSRDGRFH
                     AYGRSSTSPQMVLVIEDTLAKKDSVFKPRESRATARNPSFAPDGGRVVFSLSDLGGHQ
                     IASVNLQGQDLKYLTNASGMNAWPVYSPDGSKIAFGSSRSGDFEIYVMNADGRDVVRL
                     TQCPGFDARPAWSLDGKRIAFTSNRDGNYDIYIVNADGSNPVNATSHPSRDDHAAWHP
                     DGRRLLFVSDRDGGSDLYLGAAPDEPPKNGPS"
     gene            complement(3289..4620)
                     /locus_tag="VHS97_19235"
     CDS             complement(3289..4620)
                     /locus_tag="VHS97_19235"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450397.1"
                     /translation="MFEIGSFPCKTCQGVSRRTFLRAGLAAPFALASAGKGTPAATEA
                     PKAKSILLIWLGGGPSHLDLFDPKPKAPAEYRGPFATIATRTPGVRFAELLPKLAARS
                     DKYSLIRSNVNFNAGHREAGSIALTGASEKSNIYPPNFGSIVSRQKRHDALPPFISLA
                     RGPIGDGDGPIQGSGGGTWGRGHDPFLIGCDEKGQLEIPGLKLASGMSPARLEDRRFL
                     LRELDRLKKDADSSQYDVWDSLHQQAIALFTSPQGVAALDLSRERAATRTAYGQSSFG
                     QSCLLGRRLVEAGVPYVQVNWSRYVEVYYTFSDYGWDTHADNFGLLAEWHGPLFDRVF
                     STLLDDLYERGLMETTLVVCMGEFGRTPRINEIGSRDHWPGCYFSIWAGGGVQPGRTI
                     GESDARGEHPVSDPITPAMVGTTMLELTGISSATRAELRVLTGERVIHELL"
BASE COUNT         1031 a         1478 c         1484 g         1020 t
ORIGIN      
        1 cccagccggc gccgatgcat cggtattttt tttcgaggta aggaatcagc tcgtaggtga
       61 tggcgtcgcc gtaagggccc aggttggccg agttgaccgc atacgaatcg tcgtaaaacg
      121 ggttcgcatg ctggatttcg atgatgatca tgcgcggata gttgggcccg gtccactcct
      181 tgtaaaactg atgggcctgt tcttgaacgg tgcggttgta gccagacacg tgaaaccgct
      241 cgctgtagtc gggcttgaga ttgggatcgg gcggctcttc gcgaaacccg ccgaatgtct
      301 ggggaaaatg cccgtggaaa atcaccagag gatagcgcgc gtcgggatga gaatcgaaac
      361 cttcgggcaa cagcacatgg gcacccagat gcatcggccg gccccagaac ttggtcagct
      421 tgtcgctctc gattcgctcg tgcttgatgt accgggtggt ctgtggatcg gtgatcggcg
      481 ggatgacctt gtcgaggcgg acgcgaatgg cacctcctga actcgggccg gcctcgatgg
      541 tgatctcgcg cggtgtgctg tagaggttac cgggcgcctt gttccattgt tgtccttcgc
      601 cgcggtccat gggcagcttg acgaccgggc caatcgcacg atgaaacgtc tcgtacctgt
      661 gcaagagcgc ctgaatccgg tacttgccgg gcggcaggtg ccgcgggctc tcgatcgggt
      721 agccgaccgc cgtcgcatta atgacggcgt cttgttcggg ttcgaggccg tcgacgtcga
      781 tgccgaagat ctgctgggtc ttggggcttt gattgatctg gaaccgcggt tcttccttat
      841 catccgtcga caggatgacc agcagcctgc cgtcgagcgg agtcttcgag agttccgggc
      901 caaagcttac ctggaacacg acctcggcgc tggcggctaa cgcctccgtt tccaatgccg
      961 gcacggagcg tgcgagtgga aaaacggaat tggcgaacaa cacaatcgct atagctaccg
     1021 cccgtgggat ccaagcgccc ggtgagtgcc ggcgcttcac tttgtaaaat tgtttcgtcg
     1081 tcatcatcat aggtacttcc gttacacctg ccagtccacg tcggcaagcg cgtcgccctg
     1141 ccacgtttgt gcagacggct cccaactttt tgacggtttt gtgtcgtagt aacgagctca
     1201 ggcccgtttg ttgctataga gagatcgggc gcccattgtg cccatcgcga gcggccagtt
     1261 ccagggggtg acgaaacgtc actcgccgat tttccgccaa ccccttgatc gttaaggtaa
     1321 gtcaccgaat tcttgtgcac ttagagtaca taaagccacg tgttcttgac gtttcccaga
     1381 ttggccctat tattatttat caccttcctg atgcatctca cgatttcttg taccccgatg
     1441 tcccaaagtc gtcgccaccc gcgaaaaggc ggcaatctcg ccgtctcggt acgggagcga
     1501 ccggggcaag ggtgtgggtc gggctttcca gcccgacgcg aatgggttct cgccgcctcg
     1561 gtacgggagc gaacggggca aggtggatgg tatgaaacgc tacggactgg tgacggttgc
     1621 tcttggttgg ctctgtttgt cggcatccgg ctgcgcgacg ccgatggttg cccgctacat
     1681 ctaccaggat gcggactttg gcgtcgtcgg cattcccgtc aacaactatc agaagaagac
     1741 cgatttccgc ggccaagccg aagggctcat gtcgcgacac ttccccgaag gctacgagat
     1801 cgtgcgcgcc gaggaggtca atgaaggcga gagaattctc gaccttggca agaagaccca
     1861 gatcgagacg gatccgaaag tggccgcgtt gaatcaaatg atcagcctgg ggagcctgaa
     1921 ccggaccaca tcgtacgaac agaaggataa gcttcagctc agagagtgcc gcatcattta
     1981 caggaaaaaa ccgctgcaca cgccgggccg ctctggccaa tttgcctcca cgaactcggc
     2041 tgctccgcca ctttacatcg accccaacga gatcttgcgg caccagatca agacgaccgg
     2101 cgatatgctc ggcaaggcga atactccctc gaaaaaaccc cacgacggcc caacaccagg
     2161 cgacgccgaa gtcaaaaagg cgacggtgga acccacctct ctcaactcga atttgtcaat
     2221 tcctgatcag ctggattcca tttttgatca gatgatcaaa tgcccgtgac aatttcgacc
     2281 ttcgaatcgt caatccagct cgtggacgtc gtcgagaact cccggcggga tgcaaatcgt
     2341 gagaaacttc gatgtggcgt tcatgctgtt cttccttatg agggcccgtt tttcggcggc
     2401 tcatcaggcg ccgccccaag atagagatca gaaccgccgt cacgatccga cacaaagagc
     2461 aaccgacggc catcgggatg ccacgcggca tggtcatcgc ggctgggatg cgacgtggcg
     2521 ttgacagggt tggaaccgtc ggcattgacg atgtagatat cgtaattgcc gtcgcgattg
     2581 ctcgtaaaag caatcctctt gccatcgagt gaccacgccg gacgagcatc gaacccggga
     2641 cactgtgtga gacgcacgac atctctgccg tccgcattca tgacatagat ctcgaaatcg
     2701 ccggatcggc tcgagccaaa cgcgatcttg ctgccgtcgg gcgaatacac gggccacgcg
     2761 ttcatgcccg acgcgttcgt caggtatttc aggtcctgac cctgcaggtt gaccgaggcg
     2821 atctggtggc cgcccaggtc ggaaaggctg aagacgaccc gtccgccgtc gggcgcgaac
     2881 gacggatttc gcgccgtcgc ccgtgattcg cgcggtttga agacagagtc tttcttcgcc
     2941 agcgtatcct cgatgaccag caccatctgc ggggaggtcg atgatcgccc gtaggcgtga
     3001 aatcggccgt cgcgggaaaa agccgggtcg aactgatggt tgacaacact cggatggagt
     3061 ctatgtcgcg agccgtcgct cagattgagg caaacgatcg ataccaggtt ggggccttcg
     3121 tggctggcga atgcgacttg gtccgcgccg ccaatgaaga cagggcacag tttgagagtg
     3181 ccgtcgtgtg tcagccggac gggctcggct cctctcgcca tggtgctagt ggaaagtgcc
     3241 acaagaatca ccagaattcg atagtaacga cgacggtgaa ggcgcggctc aaagcagttc
     3301 atggatgact cgttctcccg tgagcacccg aagctcggcg cgggtcgccg agctgatgcc
     3361 ggtcagttcg agcatggtcg taccgaccat ggccggcgtg atcggatcgc tgacaggatg
     3421 ctcgccccgg gcgtcactct cgccgatcgt ccggccgggt tgcacgccac cgccggccca
     3481 gattgaaaaa taacacccag gccagtggtc gcgcgaaccg atttcgttga tccgcggggt
     3541 ccgtccaaat tcccccatgc agaccaccag cgtcgtttcc atcaatccgc gttcgtaaag
     3601 atcgtcgagc aaggtcgaaa acacccggtc gaaaagcggg ccgtgccatt cggcgagcag
     3661 gccgaagtta tccgcgtgcg tgtcccaacc atagtcggag aatgtgtaat acacctcgac
     3721 gtagcggctc cagttgacct gtacataggg aacgcccgcc tcgaccaggc gtcggcccag
     3781 caagcagctc tggccgaaag agctttgacc atacgcggtg cgagtcgctg cgcgttcacg
     3841 tgagaggtcg agcgccgcta cgccctgggg cgatgtgaac agggcaatcg cttgctggtg
     3901 caagctatcc cagacatcat attggctgga gtcagcatcc ttcttcaggc ggtcaagctc
     3961 acgaagcaag aagcgtcgat cctccagccg cgcagggctc atcccgctgg ccagcttcag
     4021 accgggaatc tcgagctgac ccttctcatc gcagccgatc aagaatggat cgtgaccacg
     4081 gccccaggtc ccgccgccgg atccctgaat cggtccgtcg ccgtcgccaa tcgggccacg
     4141 cgccagcgag ataaacggtg gcaaggcgtc atgccgtttc tgccgcgaca cgatcgagcc
     4201 gaaattgggc gggtagatgt tgctcttctc gctcgcgccc gtcaaggcga tcgagcccgc
     4261 ctcacgatgg ccggcgttga agttgacgtt gcttcggatc aacgaatact tgtccgatcg
     4321 ggccgccagc ttgggcaaga gctcggcaaa acggacaccg ggcgtgcggg tcgcaattgt
     4381 ggcgaacggc ccccggtatt ctgccggtgc cttgggtttc gggtcaaaga gatccagatg
     4441 gctcggaccg ccccccagcc agatgagcaa gatcgacttt gcttttgggg cttcggtcgc
     4501 ggcaggcgtt cccttgcctg ccgacgccag tgcgaatggg gcggccaagc cggcccgcag
     4561 aaatgtccgg cgacttaccc cctggcaggt tttacacgga aagctaccta tctcgaacat
     4621 cgccatggac cccttagcgg ccgatttgtc gttgttctca ccgcgtaccg gctcatctta
     4681 acctgtcccc agcctcaact cattggaatt ctgatccgtc agagccagcc tcaaacgaag
     4741 cgctcggtct tgattgacag tgtcatcgtc ccagttgccg atccttcgac atagcggacc
     4801 gtccaagctt gctgtcgcga gaccaaattg ttgtagcttc gggcgatgtc ggcgcggtgg
     4861 tcggtgcgcc gtcccggcct caatcgccct gcaaacccgc aaacggtgtg caaacaacct
     4921 ccgaatcgac taataatggt tctggaacaa aaaatggcga agacttccaa cgaagtccaa
     4981 cactgagcca gaggaaacct cgatgattca tgg
//
LOCUS       DATAQL010001115         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2861676, whole genome shotgun sequence.
ACCESSION   DATAQL010001115 DATAQL010000000
VERSION     DATAQL010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2861676"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(923..2740)
                     /locus_tag="VHS97_19240"
     CDS             complement(923..2740)
                     /locus_tag="VHS97_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3450398.1"
                     /translation="MPSSLSAADFQAVLDRAEQTAGRPGGPSPSPEDWRDLPIYFLMV
                     DRFNNPTAGPRHAPFDDPGFADYQGGKFSGVQAQLPYIKALGAGAVWLSPVLKNLPFD
                     PSYHGYGIHDFISAEPRFANNPAKADDELRALVDAAHAQGLYVIFDIVLNHTGDVFAY
                     QCDPQDGLCNSTGGGKASFHDGAMGVQWRDASGVAQAAFTDVANVPSPSRDAFVWPME
                     LQQNRFFRRQGVSGGSDDTVGDFDILKQILTVDADAQRFLIRAYQYVIARFDIDAYRI
                     DTLRYLKGNLAQTFGNAVREFALSIGKKNFFTFGEVLDGRAEEDIARFIGRNTSDRGD
                     QVGVDAALDYPLFNTLRPVVKGQAAPEALVGMYQRRKEIEWDILSSHGDATRFFVTFL
                     DNHDMKERIRYVDPADPHKFDDQVTLGLGCLYSLPGIPCLYYGTEQLLHGRGSDEAVR
                     EALWGGPGFDQTSPFYQQIAALAATREARPALRYGRFYFRPVSADGATFGITTFTQGV
                     LVFSRILNDQEVVVAANTNTASDQGQEVDVIVDGALNADGAAYMVLYSNKPAPTPPGP
                     VNRVTSSVTVNEVDGSTGHGPLSVIRVTLGPMEVQILGR"
     gene            complement(3252..>3458)
                     /locus_tag="VHS97_19245"
     CDS             complement(3252..>3458)
                     /locus_tag="VHS97_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450399.1"
                     /translation="PKREVLHFVALVCLAQTADVPRRLGEQVKSTRSPRSRVGLGTGG
                     VRQKAAVGAMGRMRRKKTFACGER"
BASE COUNT          652 a         1024 c         1102 g          680 t
ORIGIN      
        1 agcaaagcga agctaataag cctaaacacc agtaaattcg ctagcgttta ctagcgttta
       61 ttttttcgaa tcgagccttt tcaatgggtt gcggacggtg taagtaggga aaatcgcttc
      121 ctcgctccag ctcgcgcggc aggttgtggg ccagcgtttc aaacagccac ggtttctcgt
      181 tttccgcccg tcaagtgacg agcggaaagc ggattccgat aagtgaaata cataccgcgt
      241 gtttctgatt ttgtcaagtc aattcgcgtc tcctccagtg atagctgttg accaagagag
      301 caccgccgag ttgtccgact tggtggccca tccgcatgcc taagatccta atgcaagtga
      361 atttcgcttc gaatacgacg gaattttgca cagtcgtacg gccgttcatt cgaaaaactc
      421 gggtgtctcc ggtcccgggc caggaactgt agccaagacc ctggatgtcc tcgcgccgag
      481 cgccccgcct ggatgcggca cgttctgggg tcaaaaccca atcgcctcgc atagcgttga
      541 ccgcttcggg gcggagagcg gacgaggccc gaaacctctc aacgtccggt ctcgacgtta
      601 accggacatc gcatgttgcg actatgaacg tcgcaagtgg ggcggatagc gcccctacac
      661 gagccgcctc gggaacgacc gcagtccgcg ccatagcagt cattccattg cgagcgcgaa
      721 atagccctcg gcgccgttgg cggtcctcca attgtctcag tgcgaacttt gcatctcggt
      781 gctaagcgaa aatggcatgg gcgatgcggt tggttgcagg gaaaacttgg atttttgacg
      841 gagttgggcc gtgtgcgcca cctgtatatc aaatggtgtt tccgaaattg gggggcctga
      901 aagaaggagg gcgcaggcga tcttaccgtc cgaggatttg cacctccatc ggcccgagcg
      961 tcactctgat gacactaagc gggccgtggc cggtcgagcc atcgacctcg ttgacggtca
     1021 cgctcgacgt gacccggttt acggggccgg gcggagtggg agcgggtttg ttgctgtaca
     1081 ggaccatgta cgccgctccg tccgcgttca acgctccgtc cacgatcacg tccacctcct
     1141 gcccttgatc gctcgcggta ttggtgttgg ctgcgacgac gacttcctgg tcgttcagga
     1201 tccgcgagaa aaccagcacg ccctgggtga aagtggtgat gccgaaggtc gcgccatcgg
     1261 ccgagacggg acggaagtag aaccggccat accgcaaagc agggcgcgct tcgcgcgtcg
     1321 cggccagcgc tgcgatctgc tggtagaacg ggctggtctg atcgaaaccc ggcccgcccc
     1381 agagggcctc gcgcacggct tcatcgctgc cgcggccgtg cagaagctgt tcggtgccgt
     1441 aatacaggca ggggatgccc ggcaatgaat acaggcagcc caggccgagc gtgacctggt
     1501 cgtcgaactt gtgcgggtcc gcggggtcca cgtagcggat gcgctccttc atgtcgtggt
     1561 tgtcgaggaa cgtgacaaag aaccgggtcg cgtcgccatg cgagctgagg atgtcccatt
     1621 cgatctcctt gcggcgctgg tacatgccca cgagcgcctc gggcgcggcc tgtcccttga
     1681 cgaccggccg gagcgtgttg aacagggggt aatcgagcgc ggcgtcgacg ccgacttggt
     1741 ccccccggtc gcttgtgttg cggccgatga accgcgcaat gtcctcctcg gcgcggccgt
     1801 cgagcacctc gccgaaagtg aagaagttct tcttgccgat gctcagcgcg aactcccgga
     1861 cggcgttgcc gaaggtctgg gcgaggttcc ccttcaggta gcggagtgtg tcgatgcggt
     1921 aggcatcgat gtcaaaccgg gcgatgacgt attgataggc ccggattagg aagcgctggg
     1981 cgtcggcgtc gacggtcagg atttgcttga ggatgtcgaa gtcgcccacc gtgtcgtcgc
     2041 tgccgccact gacgccctgg cggcggaaga agcggttctg ttgcagctcc atcggccaga
     2101 cgaaggcgtc acgcgacggg ctggggacgt tcgcgacgtc agtgaacgcc gcttgcgcca
     2161 cgccgctggc gtcgcgccat tggaccccca tggcaccgtc gtggaagctc gccttgccgc
     2221 cgccggtgga gttgcagagg ccgtcttgcg ggtcgcactg gtaggcgaac acgtcgcccg
     2281 tgtggttcag gacgatatcg aagatgacat agagcccctg cgcgtgcgcg gcatcgacca
     2341 gggcgcgcag ctcgtcgtcg gccttcgccg ggttgttggc gaaccggggc tcggccgaga
     2401 tgaaatcgtg gatgccgtaa ccgtgatacg acgggtcgaa gggcaggttc ttaagcacgg
     2461 ggcttaacca gacggccccc gcgcccaggg ccttgatgta gggcaactgc gcctggacgc
     2521 cggagaactt gccgccctgg tagtcggcga agccgggatc gtcgaaaggc gcgtgcctcg
     2581 gcccggccgt cgggttgttg aaccgatcga ccatcaagaa atagatcggc aggtcgcgcc
     2641 agtcttcggg cgacggggag ggcccccctg gacgtccggc cgtctgctca gcccggtcga
     2701 gcacggcctg gaaatcggcc gcggaaagcg aagatggcat ggtaggcctt ctccaaagat
     2761 cgtgtatcga atgatcgcgg tttcgaggac gtccggggct cgaaaaccgc aaagtcaaag
     2821 gcgacttcta gacagattag cgcctagatg cgctttggtc atggtacatg ttcggggcac
     2881 cggtctttac accgagtcga cgtcgaggcg gcccattcac aagcgccgac ggaagcgacc
     2941 ctctccaatg cgagcgtacc gggcaggata cgctcttttc gtcctcccaa atgggatcga
     3001 actcacactc cgagtcgctg ctcacccaag cggttccagc aattacgcga cgttggaaca
     3061 ttgggatcgc gcatgactcg aatcgccgga ttcgaacggg accagcttca gttgttgaac
     3121 gagcgggtgg acaatttcgt ctgggtcgac aatccggtgg ggttcatcct tcgtagatgg
     3181 gcttgatctt tcgaactcca ccagcagccg aggccgagac ccacctaagc ctcacagtgt
     3241 agtattgagg ctcatcgctc gccgcacgcg aatgtctttt tacgacgcat cctgcccata
     3301 gcgcctacgg ccgctttctg gcgcactccg cccgtaccga gtccaactcg ggaacgcggg
     3361 cttcgggtcg atttgacctg ttcgccgagc cgccgcggaa cgtccgctgt ttgcgccaag
     3421 cagaccaagg cgacgaaatg gagaacttcc cgctttgg
//
LOCUS       DATAQL010001116         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_265943, whole genome shotgun sequence.
ACCESSION   DATAQL010001116 DATAQL010000000
VERSION     DATAQL010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265943"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VHS97_19250"
     CDS             complement(<1..786)
                     /locus_tag="VHS97_19250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450400.1"
                     /translation="MVGLGLADVALVAWYIGVLVLLEGLLSADNALVLAVMVRHLPKN
                     QQRRVLFYGIWGAVLFRVLALSLSSILLSLWYCKVIGGGYLLYLALAHFLWHRDSGPS
                     DGQAAVPTSRPNRWLRGFWGTVTSITLADIAFSIDSILAAVSLADGFPDHFGNRGKLF
                     IVFTGGVLGIITMRFVVRYFLILLERFPGLEEGAYYLVAWIGLKLMISGFYSGKFIKF
                     HIPEWLFWLVMVLIAAASLLIRPGAKKTDGDSGLSERLDLLDSV"
     gene            1261..2958
                     /locus_tag="VHS97_19255"
     CDS             1261..2958
                     /locus_tag="VHS97_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose transferase"
                     /protein_id="HEX3450401.1"
                     /translation="MRSSPGPGSKRERAKNLSIGRLALVMGIVAGLAVVLTLDGPGLT
                     IDEPLDVRPGRTYVDVLRQEGWHFFDRSAVDRVFGDNAEHPPLGRWLLGIASELGEPI
                     EVLWKGPDPTGHYVLAGRLAPAFAFAALVSLVTLVAGRRWGPVAGAAAGFALLAMPRV
                     FAHAHLAALDTFLSLFWTLALIAGDRALRAKRPVVAMAAAGLVWALALLTKIHAWFLW
                     PILTAWACVLLPPRRALVAMSVWTTVGICLYALGWPWLWYDSWHRIAAYWGTGVARSS
                     IMVQYFGQVVADRDVPWHYPWFYFAVTVPVGLLALGAVGVARGWRERRADLFPLLLLA
                     TIVVFLGLFSTRVPVYDGERLFLHVFPAWALLVGHGFGWLWDRQITSRTGRAVLGGFL
                     IIQIYGTLALHPFGLSYYSALIGGLRGAERAGLELTYWNDPVDQVLLDRLAREAHAGA
                     TAALAPTLYPGQGILTTNRAMARRNVILRDEDEGLRSEWLVLSRRTAYWHAQLIERLR
                     NGDGRLVAVRSRQGVWLAALWHFPSSGHQKREEKSDGGATRRHSDVSAKSPVAKTAQ"
     gene            3055..3597
                     /gene="moaC"
                     /locus_tag="VHS97_19260"
     CDS             3055..3597
                     /gene="moaC"
                     /locus_tag="VHS97_19260"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX3450402.1"
                     /translation="MGDLTHFDESGASRMVDVAGKEVTLRTARASGRIKLEPSTLSLV
                     MDRRLAKGDVFEVARLAGIMAAKRTGELIPLCHPLGLDSVEIALLPVEPDEIQIEAVV
                     KVHGRTGVEMEALTAVSVAALTVYDMCKSVDRGMVIACIQLEEKTGGRRGAFRRLELS
                     ALDPAHESDSSVHGRETAHP"
     gene            3618..4625
                     /locus_tag="VHS97_19265"
     CDS             3618..4625
                     /locus_tag="VHS97_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX3450403.1"
                     /translation="MSLHSVVEVGENRRNLSSMYAPIAAELAEAERIFQIELGSHFPF
                     VQRLVDHCADFQGKRLRPALLLLSAKASGQTSPAHPVLAAVVEMIHTATLVHDDVLDE
                     AVIRRHAATVNAEWGNETAVLLGDYLFTHAFHLAASLDSTRACRWIGHATNKVCEGEM
                     QQVHHRGNLDLDENDYFAIIEGKTAELTAVSCRLGASYAGAPESTVLALDGYGRNLGI
                     AFQIADDVLDIWGEERVTGKSLGTDLEKQKLTLPLIHMLKRVEPTAAATVRRWLSEAD
                     PELRRRIRPHLEKTGALDYSWQCAKQHAKQAADALDCLADSDAKTVLRSLAHYVVRRV
                     S"
     gene            4954..5421
                     /locus_tag="VHS97_19270"
     CDS             4954..5421
                     /locus_tag="VHS97_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3450404.1"
                     /translation="MELKDKLQMLMARAALNGQKLARLSRVSDSEISRILQGKSRPGL
                     DNAFRLARSVGVTLDYLADDSLDVEPPKPDDHLSSDERRILTLAQKVGCSQVVTILEN
                     IRFLGYEVAMSRLVGGKPIIEVDKEIGELRSNASPPVVTAGGVVSPRASSATA"
     gene            complement(5462..>6765)
                     /gene="rpoN"
                     /locus_tag="VHS97_19275"
     CDS             complement(5462..>6765)
                     /gene="rpoN"
                     /locus_tag="VHS97_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX3450405.1"
                     /translation="LEDAAKSATSEVDAEEMTPATKETEPGTEPNEFDTLIGLDENWS
                     EIYDEGPRRSRASLSEESDRKQEAMQNMASRPRSSHDDLTDQLGFFDCEPIVRDLAEY
                     IVHNLDEVGLFKIDLLDVVRDFNGRATVEEAEEALRLVQRLDPPGVGARDLRECMLLQ
                     LTPEIPCHDVLHTLISQHFDDLGHNRLPAIEKKTGIPIEKIKEALEHLRRLNLRPGAG
                     FESDSSRYVIPDLIVENNEQGGYDVRLSDENSPQLGISGYYQKLLRNKQTSPETREYI
                     QKKIQSARWLIDSIEQRRNTLLKVARAIIEHQRDFLDKGPEFIEPLKMQQIADKVGVH
                     VTTVSRAVDDKWVQTPRGIFALKRFFGGGTITADGDEIAWDTIKQKLLEIIAKENKQN
                     PMSDEEIVDEMERQGLKVARRTVTKYRQNLQIPSSRQRRQF"
BASE COUNT         1300 a         1985 c         2062 g         1418 t
ORIGIN      
        1 taccgagtcg agcaaatcca gcctttcgga caggccgctg tcgccatccg tttttttcgc
       61 gcccggcctt ataagcagac tcgccgccgc aatcagaacc atgaccagcc agaagagcca
      121 ctcgggtata tgaaatttga tgaatttccc actgtaaaat ccgctgatca tcaacttaag
      181 tccgatccac gcgaccaggt aataagcacc ctcttcgaga ccgggaaaac gttccagcaa
      241 tatcaggaag tagcgcacca caaatcgcat ggtgatgatg cccaataccc caccggtgaa
      301 aacgataaaa agcttgcccc gattcccaaa atggtctgga aagccgtcgg ccaagctgac
      361 cgctgcgaga atcgaatcga ttgaaaaggc aatgtctgcc agtgtgatcg aggtgaccgt
      421 accccagaat ccacggagcc acctatttgg tctggatgtg ggaactgcgg cttggccatc
      481 ggatgggcct gaatcgcggt gccaaagaaa atgggccagc gccagataga gaaggtaacc
      541 accaccgatc accttgcagt accagagtga cagcaatatc gatgacagcg acaaggccag
      601 gacccggaac aagacggcgc cccagatccc gtagaaaagc acacggcgct gttgattctt
      661 ggggagatgg cggaccatga ccgcgagcac cagcgcgttg tcggcgctca aaagtccctc
      721 aagcaacacg agcacgccga tgtaccaggc gaccagtgcg acgtccgcga gtcctagacc
      781 caccatgctg cttgacctcg tcgggttcgc gccccgcacc gagccggcgc tggccgtttg
      841 cggcggacga cccaaggaac aacctattgg tcggatgaaa acgaagggtc aatagtaacc
      901 ataacgcgaa ggcctgggcg acagtgcggt cgggcgcacc gacacaatcc attcgggatc
      961 gcgcgtttga atatttctgt gatctggatg gatccacgca gttctcatcg gcacgactga
     1021 tcgaacctgg atggtcaggc tcatgcaacc actagtagtg tcaaccgctg cccgttcgcg
     1081 gatgaatact tggacttccc gcactcggct ccggatcgtt tgtcggccga gcgaaatacg
     1141 tcacgtgaac cttcgggcat ggaatgcgct gtattcttgg cgaattcaaa gaagaataca
     1201 ctgagccccg atgtccgcga gtcgtcagcg atgaatccag tcagtcaaga tgtttccgcg
     1261 atgagatcct cgccagggcc cgggtcaaag cgggagcgag ccaaaaacct ttcgattggt
     1321 cgccttgctt tggtgatggg aattgttgca ggtctagcgg ttgtcctcac cctcgatggc
     1381 cctggtctca caattgacga gccgctggac gttcggccgg ggcggacgta tgtcgacgtc
     1441 ctgcgccaag agggatggca cttcttcgat cgctcggccg tcgatcgagt ctttggcgac
     1501 aacgccgagc atccgcccct gggtaggtgg ctgctcggca ttgcgtcgga gctcggcgaa
     1561 ccgatcgagg tgctgtggaa ggggccggac ccgacgggac actacgtcct cgccggcagg
     1621 ctggcccctg cattcgcgtt tgccgcgctt gtgagcctgg tcacgctggt ggccggccgg
     1681 cgctggggtc cggtggcggg ggcggcagca gggtttgcgc tcctggcgat gccgcgggtg
     1741 tttgcccacg ctcatttagc ggctctcgat acgtttctga gcctgttttg gactctcgct
     1801 ctgatcgccg gagatcgcgc gctccgcgcg aagcggccag tggtcgccat ggctgcggcg
     1861 ggcctggtgt gggcactggc gctgctgacc aagatccacg cctggttctt atggccgatt
     1921 ctcacggcat gggcatgcgt gctcttgccg ccgcgacgcg cgctggtcgc gatgtcggtc
     1981 tggacgaccg ttggaatctg cctgtacgcc ctgggctggc cctggctctg gtacgactcc
     2041 tggcaccgaa tagcggctta ttggggcacg ggcgtcgcgc ggtcgtcgat catggtccag
     2101 tacttcggcc aagtcgtcgc cgatcgtgac gtgccctggc actacccctg gttttatttc
     2161 gcagtgaccg ttcctgttgg cctcctcgcc ctgggagcag tcggggtcgc acgaggatgg
     2221 cgagaacgcc gcgccgatct gtttccactg cttctgttag ccacgattgt ggtttttctg
     2281 ggcctcttca gcacccgcgt ccccgtctat gacggcgagc gtctgtttct tcatgtcttt
     2341 ccggcctggg cgcttctggt cggacacggt tttggctggc tctgggaccg ccagatcaca
     2401 agtcggactg gacgcgctgt actcggggga ttcttgatca ttcagatcta tggcaccctg
     2461 gcgctacacc cgtttgggct cagttattat agtgcgctca tcggcgggtt gcggggggcc
     2521 gagcgggccg ggctggagct gacgtactgg aacgacccgg tcgatcaggt tttactcgac
     2581 cggctggcgc gcgaagcaca cgccggcgct acggccgcgc tggcgccgac actgtatccc
     2641 ggacagggaa ttctcacgac caaccgcgcc atggcccgac gcaatgtgat tctgagagac
     2701 gaagacgaag ggctccggtc cgaatggctg gttttatcgc gtcgcacggc ttactggcac
     2761 gcccagctga ttgagcgact gcgcaacgga gatggccgat tggtggccgt ccgatccagg
     2821 caaggcgtct ggttagccgc cttgtggcac tttccttcga gcggacacca aaagcgcgag
     2881 gagaagagcg acggcggcgc gactcgacga cacagcgatg tatcggcaaa gagcccggtc
     2941 gccaagaccg ctcagtgaag ggacttgtga aacttggcct cttccctcta gccgtcgcgc
     3001 cgacgattcg tagaatactt tcagaatgaa ttgaacgaaa tccgaggttt gtccatgggc
     3061 gacttaacgc acttcgatga gagcggagcg agccgcatgg tcgacgtggc gggcaaggag
     3121 gtcacgctcc ggacggcccg cgccagcggc cgaatcaagc tcgaaccgtc aacactgtcg
     3181 ctagttatgg atcggcggct cgccaaagga gatgttttcg aagttgctcg cctcgccggg
     3241 atcatggcgg ccaagcggac cggtgaattg atccccctgt gccacccgct ggggctggac
     3301 agcgtcgaaa ttgcgctttt gccagtcgag ccggacgaga ttcagattga agccgtcgtc
     3361 aaggttcatg gccgcaccgg cgtcgaaatg gaagccctga cggcggtgag tgttgctgcg
     3421 ttgaccgtgt acgacatgtg caagtcggtc gaccgcggaa tggtgatcgc ctgcattcag
     3481 ctcgaggaaa aaactggcgg gcggcgcggg gcgtttcggc gactcgaatt gtccgcgctc
     3541 gacccggctc acgagtccga ttcgagcgtg cacgggcggg aaacggctca tccgtaagat
     3601 gccgtgggag gggtcgaatg tcgttgcatt cggtggtcga ggttggggaa aaccggcgta
     3661 acttgtcgtc gatgtatgct cccatcgcgg cggaactcgc cgaggccgag cgaatcttcc
     3721 agatcgaact ggggagccac ttcccgttcg tacaacgcct ggttgaccat tgcgcggatt
     3781 tccaagggaa gcggctgcgg ccggccctgt tgctcctctc ggccaaggca tccggccaga
     3841 cttcccctgc tcatccggtg ctggcggcgg tggtcgagat gattcacacc gcgacgctcg
     3901 tgcatgacga cgtactcgat gaggcggtga tcagacggca tgcggcaacc gtgaatgccg
     3961 agtggggcaa cgaaaccgcg gtcttgctgg gggactatct cttcacgcac gcctttcacc
     4021 tggccgcctc tctggattcg acgcgtgcgt gccgatggat cggccacgcg accaacaagg
     4081 tctgcgaagg tgaaatgcag caggtccacc atcgtggcaa tctcgacctc gatgagaacg
     4141 attatttcgc catcatcgag gggaagacgg ccgagttgac cgctgtctcg tgccgcctgg
     4201 gggcctcgta cgccggcgca cccgagtcga ccgttctcgc gctcgacggc tacggacgta
     4261 acctggggat cgcgtttcaa atcgccgatg acgtcctcga catctggggt gaagagcggg
     4321 taacaggcaa gagcctgggt accgacctcg aaaaacaaaa gctgaccctt cctttgatcc
     4381 acatgctcaa aagggtcgaa ccgacggccg cggcgaccgt ccggcgctgg ctgagcgaag
     4441 ccgatcccga gttgcgccgg cgcatccgcc cgcatctcga gaagaccggt gcactcgact
     4501 actcctggca atgcgccaag cagcatgcga agcaggctgc cgatgcgctc gattgcctgg
     4561 cggactctga cgccaagaca gtgcttcgat cgctcgcaca ctacgtggtc cggcgcgtgt
     4621 cttagcgcag ccggctcccg cggaagttgt cgcggcacat tcgcccggtt tcgccctgga
     4681 gagcgatgcc ccaagccgcg tgccggccgt ccctccagtg taaatcgaca ctccttcgcg
     4741 cgagtgtttt ttgaatccga attgcatctc gcggatcatg ggttatcatt ctccgtcgtc
     4801 gaccaacgat cgaccccgat tatgtcggcc cagatcgaca ctcgccaccg acgcaaccat
     4861 ggcggcgtgt ggcgctgctt catgagcggg aaatttatgc aaatctgaaa ttcttcagtt
     4921 ccccacatcg gggacgaatc gtatggtcga gacatggaac tcaaagacaa attgcaaatg
     4981 ctgatggccc gtgcggcctt aaacggtcaa aagctggccc gattatcccg ggtcagcgac
     5041 tcggaaatct cgcgcattct tcaaggcaaa tcgcggccag gcctggacaa cgcatttcgg
     5101 ctggcgagat cggtcggtgt aacgctcgac tatctcgccg acgacagcct ggatgtcgaa
     5161 ccaccgaagc ccgacgatca tctctcgtcg gacgaacgga gaatcttgac gctcgcccag
     5221 aaagtcggtt gttcgcaagt agttacgatc ctcgaaaaca tccgattcct tggctacgag
     5281 gtggcgatga gccgcctggt cggcggtaag ccgatcatcg aggtggacaa ggaaattggc
     5341 gaactccgtt cgaacgcatc gccgccggtc gtgacggcag ggggcgtagt atctcctcgc
     5401 gccagttccg ccacggcgta acagaatgtc gtctggcccg cctgcagccg gagattgatc
     5461 gctaaaattg cctgcgctga cggctcgacg ggatttgcag attttggcgg tatttcgtga
     5521 ccgtccgccg cgcgactttt agaccctgac gttccatctc gtcgacgatc tcttcgtctg
     5581 acatcggatt ttgcttgttt tccttggcga tgatctcgag cagcttttgc ttgatggtgt
     5641 cccaggcgat ctcgtcgcca tccgccgtga tggttcctcc gccgaagaat cgcttgagcg
     5701 cgaaaatccc gcgcggggtt tgaacccatt tgtcgtccac cgcccggctc acggtcgtga
     5761 cgtggacgcc aactttgtcg gcaatctgtt gcatcttgag cggctcgata aactcagggc
     5821 ccttatcgag aaagtctcgt tgatgctcga tgatagcccg cgccaccttg agtaacgtgt
     5881 ttcgccgttg ttcgatcgag tcgatcagcc agcgggccga ttggattttc ttctggatgt
     5941 attcacgggt ctccggagaa gtttgcttgt tacgcagtaa tttctggtag tagccggaga
     6001 tacctagctg gggtgaattc tcgtcggata gccggacgtc gtatcccccc tgttcgttgt
     6061 tttcgacaat caggtcgggg atgacgtagc gagaactatc cgattcgaaa ccggcacccg
     6121 ggcgcaggtt aagtcggcga aggtgctcaa gcgcctcctt gatcttttcg atcggaatcc
     6181 ctgtcttttt ctcgatggcc ggcaagcgat tgtgacccag gtcgtcgaag tgctgcgaga
     6241 tcagggtgtg gaggacgtca tgacagggga tttccggcgt caattgcagc aacatgcatt
     6301 cccggaggtc gcgagcgccc acgccgggcg ggtcgagtcg ctggaccaat cgtagcgctt
     6361 cttcggcctc ttccactgtc gcccggccgt tgaaatcgcg aaccacatcc aagagatcga
     6421 tcttgaatag gcctacttcg tcgaggttgt gcacgatgta ctcggccaga tcgcggacga
     6481 tcggctcgca atcgaaaaaa ccaagctgat cggtcaggtc gtcgtgggat gaccgcggtc
     6541 gggatgccat attttgcatg gcttcctgtt tgcggtcgga ttcttcgcta agagatgcac
     6601 ggctgcgcct cggcccttcg tcgtaaatct cgctccagtt ttcgtcgagc ccgatcaagg
     6661 tgtcaaactc gttgggctcg gtgcctggct ctgtctcttt cgtcgcaggt gtcatctcct
     6721 cggcatcgac ctcggatgtg gcggacttgg ccgcatcctc gagaa
//
LOCUS       DATAQL010001117         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2711157, whole genome shotgun sequence.
ACCESSION   DATAQL010001117 DATAQL010000000
VERSION     DATAQL010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711157"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VHS97_19280"
     CDS             <1..992
                     /locus_tag="VHS97_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450406.1"
                     /translation="FNNTPAGKAPNRFAPLLVQKLQSRPYTPLIKQIFGPDVFTKFTP
                     RQIYEIWAEAIAAFEASGEICAFSSKYDASPFGVPPQNLYTLSASEERGRQLYFGNAQ
                     CFACHSSLTLPDVQIETTGKNTFTMYCFANIGVPKNPGNPYYEETDPVSNPNGYNPLG
                     RNFIDWGLGGNAVGSLDGTKFFNTTPGDIAQFRGLFQTPTVRNSDQRPYPTFVKSYMH
                     NGVLKSLLEVVHFYNTRNIAVDANGNQVAFDLTVGPPTGYTAIWPPPEVLDNVQNVAG
                     YTPAQAAAAGTTGVTAQNGQVGNLGLTASQEADLVNFLKILSDGFTAPNPVGN"
     gene            complement(1236..>1623)
                     /locus_tag="VHS97_19285"
     CDS             complement(1236..>1623)
                     /locus_tag="VHS97_19285"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX3450407.1"
                     /translation="WTRVILPRMRVFRRMLDRIAWDGMFRGRFEDRAFAIDAFNRHND
                     LVRRDVPAERLLVYQVSEGWEPLCAFLGVAVPEGKPFPHLNDAADFRARVERGVRVIR
                     AIEYVALGVIALAMVSLVMLAIRLGS"
BASE COUNT          315 a          564 c          480 g          264 t
ORIGIN      
        1 tcttcaacaa cacccccgcg ggaaaggccc ccaaccggtt tgccccgctc ctcgtccaga
       61 agctccagtc ccggccctac accccgctga tcaagcagat cttcggcccc gacgtgttca
      121 ccaagtttac gccccggcag atctacgaga tctgggccga ggccatcgcg gcgttcgagg
      181 cgtcgggcga gatctgcgcg ttcagctcga agtacgacgc ctccccgttc ggagtgccgc
      241 cgcagaacct gtacactctc tccgcatccg aggagcgcgg gaggcagttg tacttcggca
      301 acgcccaatg cttcgcatgc cactcgtcgt taaccctgcc ggatgtccag atcgaaacga
      361 cggggaagaa cactttcacg atgtactgct tcgccaacat cggcgtcccc aagaatccgg
      421 gcaatcccta ctacgaggag accgatcccg tcagcaatcc caacgggtat aaccccctcg
      481 ggaggaattt catcgactgg ggcctcggtg gcaatgcggt cgggtcgctc gacgggacca
      541 agtttttcaa cacgaccccg ggcgacatcg cccagttccg gggtctgttc cagaccccga
      601 ccgtgcgcaa ctcggaccag cggccctacc caacgttcgt gaagtcatac atgcacaacg
      661 gcgtcttgaa gagcctgctg gaggtggttc acttctacaa caccaggaac atcgcggtgg
      721 acgccaacgg caaccaggtg gccttcgacc tgacggtcgg cccaccgacc ggctacacgg
      781 cgatctggcc accgcccgag gtgctcgaca acgtgcagaa cgtggccggc tacacccccg
      841 cccaggcggc cgccgccggg acgaccgggg tgacggcaca gaacggtcag gtaggcaatc
      901 tcggtttgac ggcctcccag gaggccgacc tcgtcaactt cctcaagatc ctcagcgacg
      961 ggttcaccgc gcccaacccc gtcggcaact gagattccgt cgccccgtct tgacacagag
     1021 ccgggacgcg gtcggacccg cgtcccggcc gactgattca ggctgtcccg gaacaaacga
     1081 catcgacgct tgcgatgaga gattgaccgt gttcgggatc ataaaatcgc cgtcgcgcat
     1141 gcttggcgat taagtcggcg ggaccggctg tcgctgcagc gcgtgtcctt tttccctttc
     1201 ctttttcccg gatcgccgcg gacggtcggc gcggctcacg atccgagccg gatcgccagc
     1261 atcacgagcg agaccatcgc cagcgcgatc accccgaggg cgacatattc gatggcccga
     1321 atgacgcgga cgccgcgctc gacgcgggcg cggaaatcgg cggcgtcgtt gagatgcggg
     1381 aacggcttcc cttcggggac ggcgactccc aggaacgcgc agaggggctc ccagccctcg
     1441 ctgacctgat aaaccaaaag ccgctccgcg ggcacatcgc ggcgcaccag gtcgttatgc
     1501 cggttgaagg cgtcgatggc gaaggcccgg tcttcgaacc ggccccggaa catcccatcc
     1561 caggcgattc ggtcgagcat ccgtcgaaag acccgcatcc gcggaaggat cacgcgggtc
     1621 cac
//
LOCUS       DATAQL010001118         5894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3471382, whole genome shotgun sequence.
ACCESSION   DATAQL010001118 DATAQL010000000
VERSION     DATAQL010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471382"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..820)
                     /locus_tag="VHS97_19290"
     CDS             complement(209..820)
                     /locus_tag="VHS97_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450408.1"
                     /translation="MSPNHSRRMSLLEAILMVGSAAVGLGLFELSHRTLFKGLIWIAD
                     RGFPNIQSWSTLEALVTCSDITVFLLPVIIPWTFLLILLRMRSPRPSWRRIWRQPGMA
                     ACLVAVFAWLWTAVALLLAMNVEHVARARRVITPAAWAQKYLSDEVFMYVGLAVAAVW
                     VVQYSSGRWRRSADWIDMMGRVVGALWIAIGLVWTLREYIEFV"
     gene            complement(907..3198)
                     /locus_tag="VHS97_19295"
     CDS             complement(907..3198)
                     /locus_tag="VHS97_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX3450409.1"
                     /translation="MISPFRYFALLVVAFLTSSLLAGEDPDPSRLTVERIFGDHEFEP
                     EHVRVRWLGDGQGYVTMDPSTDAAGGRDLVRHDPRTGEPTVLVPASALIPPGADAPLP
                     IDDYAFSSDQARLLIFTNSQRVWRTNTRGDYWVLDRSSHELHKLGGDAPPASLMHAKF
                     APGGLRVAYVRSNNIYVEDVRDHKITQLTSSSSPDVINGTFDWVYEEEFSLRDGFRWS
                     PDGLAIAYWQLDTTGVREFPLVNNTDSLYPRINPIKYPKVGETNAACRVGVVAAAGGE
                     TRWLNVPDDPRNHYIAFMEWAGNSNDIVLQQLNRHQDNVRVMLAHQNSGSVATVLTER
                     DNAWVDLQDKLHWIEDGKEFLWMSERDGWRHVYRVGRQGGSKPVLITPGEFDVIDLLA
                     VDQKSSWVYFTASPDNPTQHYLYRVHLDGSGRERVTPTNQPGSHDYQISPDANWAIQR
                     SSRFDKVPTTTMVALPTHESTRILSDNKSLKDKIDGLKKGRTEFFRVDIGAGIALDGW
                     CILPPDFDSNRKYPLLVHVYGEPAGLTVLDRWGNSNHLWHVMLAQSGYIIMSFDNRGT
                     PAPRGRAWRKSVYHQVGVLAPKDQAAALKEVLRTRPYIDQSKIGIWGWSGGGSMSLNA
                     IFKYPDLYKTAIAVAPVANQRYYDTIYQERYMGLPGENVEGFTQGSPINFAHQLKGNL
                     LLIHGTGDDNCHYQATEALINELVRHNKPFSMMAYPNRSHAINEGRNTTLHLRELMTR
                     YLKDNLPPGAIAKPKVQPAVTEK"
     gene            complement(3238..4341)
                     /locus_tag="VHS97_19300"
     CDS             complement(3238..4341)
                     /locus_tag="VHS97_19300"
                     /EC_number="4.2.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF043002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901605 - alpha-amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cis-3-hydroxy-L-proline dehydratase"
                     /protein_id="HEX3450410.1"
                     /translation="MKITRILAYRVELPLHEGSYKWSGGKSVSVFDSTVVAVETDQGI
                     TGYGEVCPLGPFYLPAYAQGARTGIAELGPHLLGENPTEIDKLNRRMDAALQGHPYVK
                     SALDMACWDILGKVTGQSVCVLLGGRYGEDFVLYRAISQESPATMAMRVAGYRAQGYR
                     RFQLKVGGDPMVDIERIRAVASELEVGDRLIADANTGWLMHDALRVVRAVRDVDVYIE
                     QPCRTYDECLTIRRHCDHPFVLDEVIDSIDPLLRGHADRAMDVVNIKISKFGGLTKAR
                     QARDLCVSLGIAMTIEDSWGGDITTAAIAHLSHSTPTEYLFTSTDFNSYVTVSTAHGA
                     PKRKDGRMSASLAPGLGIEPRKEVLGQAAVEVK"
     gene            complement(4604..5305)
                     /locus_tag="VHS97_19305"
     CDS             complement(4604..5305)
                     /locus_tag="VHS97_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450411.1"
                     /translation="MTSFVGLFQLADNLLRGEVSAAGGSREPRLPVLGSLILAIVVYG
                     MFYGAVMGSYGGMSGLRFWQAVYSAVKVPFLMISTFLLSLPSFFVLNTLLGLRDDFPR
                     VVRALISTQAGLTVILSALAPFTAFWYISGSDYEPAILFNGMMFAVASFSAQWMLRRE
                     YAPLIRSNPRQRWMLRTWIIIYIFVGIQMGWVLRPFIGNPRAPVQFFREGSWSNAYEV
                     VLQMIWDVLNGRSRS"
     gene            complement(5328..>5894)
                     /locus_tag="VHS97_19310"
     CDS             complement(5328..>5894)
                     /locus_tag="VHS97_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450412.1"
                     /translation="VKVPLLFYLTLLVTLPSLYVFNALVGSRLTLATVVRLLIASLGV
                     MVAVLASLGPIVAFFSVSTTSYPFMLLFNVVVCSISGGLGLLFLMQTLHRLSVLDSRP
                     PAAQNSEGGDVTEPVGPLDPLESRVLGKHVKTVFQLWVIVFALVGAQMGWVLRPFIGN
                     PDLPFVWFRGRESNFFQAVVRTFQNLFS"
BASE COUNT         1287 a         1746 c         1723 g         1138 t
ORIGIN      
        1 gcactttggc cagatccgcg ccggcctcgc gtcgatcgag gcccagttga agctcttgtc
       61 ccaaacaacc aaggcccctc cggccggtcc tccgagctaa ccgggtacgt tcgccagggg
      121 ttgagcaggt tccggcaata caccggtttc gcggtgaatt gagttcgtct catcggccaa
      181 gttgcaggaa cctggtccgc ggcttcactc acacaaactc gatatattcg cgcagtgtcc
      241 acaccagccc gatcgcgatc cagagcgcac ccacgacgcg ccccatcatg tcgatccaat
      301 cggccgaacg ccgccagcga ccactcgagt actggactac ccatacggcc gccaccgcca
      361 ggccgacgta catgaagacc tcgtcggaca aatacttttg tgcccaagcc gcaggtgtga
      421 tcacccgtct agcccgagcc acatgttcga cattcatggc caagagcagc gccacagcgg
      481 tccataacca ggcaaaaacc gcgaccaagc aagccgccat ccccggctgc cgccagattc
      541 gccgccaact tggccgcggc gatcgcatcc gcagcagtat cagcaaaaac gtccacggta
      601 tgattaccgg cagcaagaac acagtaatgt cggaacacgt gaccagagcc tctaacgtgg
      661 accaactctg aatattggga aaccctctgt cggcaatcca gatcaaccct ttgaacaacg
      721 tccggtgcga tagctcgaat agtccgaggc cgaccgccgc cgagccaacc atcaagatcg
      781 cttcgagcaa gctcatcctt cgactgtggt tcggcgacat cgacgtgccc tccatctggc
      841 caaccagtaa tcgacccgac ttgtccggca taatttgcgg cctgcgattg ggaagacacg
      901 gaatccttat ttctcagtta cggcgggctg taccttcggt ttcgcgatcg ctcccggagg
      961 aaggttgtct ttcagatagc gagtcatcag ctcgcgaagg tgaagcgtgg tattgcgccc
     1021 ttcgttgatg gcgtgggatc ggttgggata ggccatcatc gaaaagggtt tgttgtggcg
     1081 gacgagctcg ttgatcaagg cctcggtcgc ctggtaatgg caattgtcgt cgcccgtgcc
     1141 atgaatcaag agcaggttgc ccttcaactg gtgggcgaag ttgatcggcg agccctgggt
     1201 gaagccttcg acgttttcgc ccggcaaacc catataacgc tcctgataaa tcgtgtcgta
     1261 atagcgctgg ttagccacgg gggcgacggc gatagccgtc ttgtagagat cgggatactt
     1321 gaagatggca ttcaggctca tcgagccgcc gccactccag ccccagatgc cgatcttgct
     1381 ctggtcgatg taggggcgcg tccgcaagac ttccttcagg gcggctgcct ggtccttcgg
     1441 ggccaggacg ccgacctggt ggtagaccga cttgcgccag gccctgccgc gaggggcagg
     1501 cgtgccgcga ttgtcaaagc tcatgatgat ataacccgat tgagccagca tcacgtgcca
     1561 aaggtggttg ctattccccc atcgatcaag tacggtcagg ccggccggct cgccatacac
     1621 atgcaccaag agcggatact tccgattcga gtcgaagtca ggcggcaata tgcaccaacc
     1681 gtcaagcgcg atgccggcac cgatgtcgac ccggaagaat tcggttctcc cctttttgag
     1741 cccatcgatc ttgtccttca atgatttatt atcagaaaga attcgcgtac tttcatgcgt
     1801 tggcagagcg accatggtgg tcgttggcac cttgtcgaat cgcgaactcc gctgaatcgc
     1861 ccagttggcg tctggtgaga tctggtagtc atgcgaaccc ggctgatttg tcggtgtcac
     1921 ccgttcacgg cctgatccat ctaaatggac gcgatagaga tagtgttgag tcgggttatc
     1981 aggcgaggcg gtgaagtaaa cccagctcga cttctggtcg accgcgagca gatcgatcac
     2041 atcgaattcg ccgggtgtga tcagcacggg cttgctgcct ccctgtcgtc caacccgata
     2101 gacgtggcgc cagccgtcac gctcgctcat ccagagaaac tctttaccgt cttcgatcca
     2161 gtgtagcttg tcctgaagat cgacccaggc gttgtctcgc tctgtcagta cagtcgctac
     2221 actgccggag ttctggtgcg cgagcatgac acgaacgttg tcctggtgcc tgttcaattg
     2281 ttgcaggacg atgtcgttcg agttacccgc ccattccatg aaggcaatgt agtggttacg
     2341 cggatcgtcg gggacattga gccagcgcgt ctctccaccg gcggcggcga cgacaccgac
     2401 cctacaggcc gcgttggtct ctcccacctt gggatacttg atgggattga tccggggata
     2461 aagtgaatca gtattgttca cgagcggaaa ttcacgaacg cctgtggtat cgagttgcca
     2521 gtaggcgatt gcaagtccgt ccggactcca acgaaatccg tcgcgtagtg agaactcttc
     2581 ctcgtagacc cagtcgaatg tcccgttgat aacgtcgggc gagctcgagc tggttagctg
     2641 tgtaatcttg tgatcgcgga cgtcttcaac ataaatgttg ttggaacgta cataggcgac
     2701 tcgcaaaccg cctggcgcaa acttcgcgtg catcagcgac gcaggcgggg catcgccgcc
     2761 tagcttgtgt agctcgtggc tgctgcggtc gagcacccag tagtcaccgc gcgtgttggt
     2821 gcgccagacg cgctgggagt tggtgaagat caagagccga gcctggtcgc ttgagaaggc
     2881 ataatcgtcg atcggcaagg gcgcatcggc accggggggt atcaaggccg atgccggaac
     2941 gagaactgtg ggttcgccgg tcctggggtc atggcgaacg aggtcgcggc cgccggcggc
     3001 atcggtcgac ggatccatgg taacgtagcc ctggccgtcg ccgagccaac gcacccttac
     3061 gtgctccggc tcaaattcgt gatcgccaaa gatccgctcg acggtcaaac gtgatggatc
     3121 agggtcttca ccggccagaa gcgaagaagt cagaaaggcg actacaagaa gcgcgaagta
     3181 gcggaatgga gatatcatcc gacaaagctc cagacgagga tcttgactgt gtcattttca
     3241 tttgacctcc acggcggctt gacccagtac ttccttccgg ggctcgattc ccagcccagg
     3301 ggcgagcgag gccgacatcc ggccatcctt ccgcttgggg gcgccgtggg ccgtgctcac
     3361 cgtcacgtaa ctgttaaagt cggtggatgt gaacaaatac tctgtcggcg tgctatggga
     3421 gaggtgcgca attgccgccg tcgtgatatc gccgccccag ctatcttcga tggtcatcgc
     3481 gatgccgagc gagacgcaaa ggtcgcgcgc ttggcgcgcc ttggtcaaac caccgaattt
     3541 gctgatcttg atgttgacca cgtccatggc gcggtctgca tggccgcgga gtagggggtc
     3601 gatcgagtcg atcacctcat cgagaacaaa cggatggtcg cagtgtcggc ggattgtcaa
     3661 gcattcgtcg taagtccggc agggctgctc gatgtagaca tcgacgtcac gcaccgcgcg
     3721 gacgacgcga agggcgtcgt gcatcagcca accggtattg gcgtcggcga tcaagcggtc
     3781 gccgacttct agctcggacg cgaccgcgcg aatgcgttcg atatcgacca tgggatcgcc
     3841 gccgaccttg agctggaaac ggcggtaacc ctgcgcgcga taacctgcca ctcgcattgc
     3901 catggtcgcg ggcgattcct gagagatcgc gcgatagaga acgaagtcct ctccatatcg
     3961 gccccccagg agtacgcata cggattggcc ggtcaccttg ccgaggatat cccaacatgc
     4021 catatcgagc gccgatttga cgtaggggtg accctggaga gcggcgtcca tgcggcggtt
     4081 cagcttgtca atctctgtcg gattctctcc caataagtgg ggcccgagct cggcgattcc
     4141 cgtgcgtgcg ccctgggcgt acgccggcag ataaaacggg cccagtggac aaacctcgcc
     4201 gtagcccgtg atgccctggt cggtctctac cgcgacgacc gtgctatcga acaccgaaac
     4261 cgacttgccc ccggaccatt tgtagcttcc ttcatgcaag ggaagctcga cacggtacgc
     4321 gaggattcgg gtgattttca tcgcgctttg gttccaggga ttcgtgaaaa cgtcctggcc
     4381 gatactagcc gaccaatcgt cagcctgcca gaggcatgga ttcggccgga ccgactgcgg
     4441 tctgccagga gaagataagc aggacgtcgt cctgggcttc aacgcagttt acgatgctgg
     4501 gatcgacggt tccctcactc aagcccctcg aatccgggtg aacgcatcga gaacgaggaa
     4561 gagaataccg cctcgcagaa ttcgccgcgg agatgcgggc atcttacgac cggctgcggc
     4621 cgttcaacac atcccagatc atctggagaa cgacttcata ggcgttgctc cagcttcctt
     4681 ctcgaaaaaa ctgaactggc gctctggggt tgccgataaa gggccggagc acccatccca
     4741 tctggatgcc cacgaatatg tagatgatga tccaagtcct gagcatccag cgttgcctgg
     4801 gattgctgcg gatcaagggc gcgtactccc ggcgcagcat ccattgggcg ctgaagctgg
     4861 cgacggcaaa catcatcccg ttgaacagga tcgccggctc gtagtcgctg cccgagatgt
     4921 accagaaggc cgtgaacggc gccagtgccg acaagatgac cgtaagacca gcctgggtgg
     4981 agatgagtgc ccgaacgacg cgcgggaagt cgtcgcgcaa gcccaagagc gtgttgagca
     5041 caaagaagct gggaagactc aaaaggaacg tggagatcat caggaacggc actttgaccg
     5101 ccgagtagac cgcttgccag aacctcaagc cgctcatccc tccatagctt cccatgaccg
     5161 cgccataaaa cattccgtag acaacaatgg ccaggatgag tgagccgagc acggggagac
     5221 gcggctcgcg tgatccgcct gccgcggata cctctccgcg caacagattg tccgcgagtt
     5281 gaaagagacc gacgaatgaa gtcatgacgg ccgacccgcg ggtttcatca ggagaaaagg
     5341 ttctgaaagg tccgcacgac agcctggaag aagttggact cgcggccgcg gaaccagaca
     5401 aaagggaggt ccgggttgcc gataaaaggc cggagcaccc accccatctg cgctccgacc
     5461 aaggcgaaca cgattaccca gagctgaaac accgttttga catgcttgcc cagaacgcgg
     5521 ctctcgagcg gatcgagcgg cccgaccggc tccgtcacat ccccgccctc gctgttttgc
     5581 gccgccggcg gtcgcgaatc gagtacgctt aaccggtgta gtgtttgcat cagaaacaac
     5641 agcccgagac cgcccgatat gctgcagaca accacgttga agagcagcat aaacggatag
     5701 ctggtggtgc tgaccgagaa aaatgccacg atcggcccca gcgacgccag cacggctacc
     5761 atcactccca acgaagcgat cagcaaacgc acgaccgtcg ccaatgtcag ccgcgacccg
     5821 accagggcat tgaatacata aagagacggc aacgtgacca gaagtgtgag ataaaacaag
     5881 agcggaacct tgac
//
LOCUS       DATAQL010001119         4501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3931474, whole genome shotgun sequence.
ACCESSION   DATAQL010001119 DATAQL010000000
VERSION     DATAQL010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3931474"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VHS97_19315"
     CDS             complement(<1..206)
                     /locus_tag="VHS97_19315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3450413.1"
                     /translation="MSQMGIVAIGRNEGERLRRCLSSVVGRDLPVVYVDSNSTDGSAE
                     LARSLGAEVVELNLSRSFTAARAR"
     gene            complement(226..1233)
                     /locus_tag="VHS97_19320"
     CDS             complement(226..1233)
                     /locus_tag="VHS97_19320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3450414.1"
                     /translation="MSSLNVLIVIVNYRTPGLTIDCLRSLEAEVRAVADWRVRVVVTD
                     NSSGDDSIPRLQAAVRDQGFSDWVSIKPLERNGGFAYGNNAAIRPALESTDPPDYVWL
                     LNPDTLVRPGALAALVDFLEHHPAVGIAGSRLVNREGTAEWSAFRFPTVLGELEGGVR
                     LGLVTRILSRWVISSPAPLVASRCDWSSGASLMIRRAVFEAIGMLDENYFMYFEEVDF
                     CRRAHRAGWAAWYVPQSEVVHLAGQSSGVTDRQRERKRLPGYWFRARRYYNKKHLGPI
                     RTLLADLAWSLGFSLYRVRQWLFRKPNTDPESLLRDFLYYNFFSNGSLSIKRPDDREL
                     R"
     gene            complement(1348..2604)
                     /locus_tag="VHS97_19325"
     CDS             complement(1348..2604)
                     /locus_tag="VHS97_19325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3450415.1"
                     /translation="MTIAYFIPTYPMPSQTFIRREIVALEAQGFTIHRFSLRRFAGVL
                     ADQADQSEEKKTCALLELGAFAIAMALVHQLVVQPRRWTIALTMAVRMGRRSDRGLIL
                     HLVYFAEACLLRRLLVDCGARHLHVHFGTNAAVTALLCRLLGGPAYSITIHGPEEFDS
                     PLALGLREKVRHAAFVVAISQFTRSQLYRWCDLADWAKVHVVRCGLDEVFLNATAVPA
                     PDQPKLVNVGRLSEQKGQQLLVEAAAGLRDRGHEFELVIVGDGPMRAEIEQQITDRGL
                     SGHVRITGFLSNQEVRRQLESARALVLPSFAEGLPVVIMETMALGRPVISTYIAGIPE
                     LVEPGVTGWLVPAGAVLPLIDAMADLLSLGSAELDQMGRSGRARIAERHVAHTEAAKL
                     AALFPNPATTGGPNHRRIAVNSVVAR"
     gene            complement(2620..3624)
                     /gene="galE"
                     /locus_tag="VHS97_19330"
     CDS             complement(2620..3624)
                     /gene="galE"
                     /locus_tag="VHS97_19330"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX3450416.1"
                     /translation="MKILLTGGAGYVGSACLRWLLARGHDPIAYDNMSEGNAAAVPDG
                     HTRLVVGDIADTDRVARVLRDQGAEAVMHFAALASVPDSISDPDAYYRVNVAGTKSVL
                     DAMRTAGVRKILFSSTAATYGFHAEMPLVEQSPQTPETPYGTTKLAAEWLIKDYARAY
                     GLGYTLLRYFNASGADPDGDFGEDRRHEAHLIPLIFHVVVGRRPKVLVYGNDYPTRDG
                     SCVRDYVHTSDLAQAHERAVLSLEPGMGRAYNLGSGTGATVLEVLRACEQIIGQPIAH
                     EIVGRRPGDPAVLIASPDMIVKELGWSPRWSDIREIVRTAWKWHQSHPHGFARVDAPQ
                     "
     gene            complement(3641..>4501)
                     /locus_tag="VHS97_19335"
     CDS             complement(3641..>4501)
                     /locus_tag="VHS97_19335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX3450417.1"
                     /translation="PRACGIAELDEVGRITAFVEKPEQPKSDLANAGLYVVNAAAYRE
                     IAAMSAFDLGFDVLPHFVGRMRGWVWGGYHLAIGTHEALERGRRDVRSNFPDLPTVSP
                     ETLQPAIFLDRDGTLIEHVHYLADPKRVRLIAGAADALRRFRRGGFARVLVTNQSAIA
                     RGMLTEEGLHEIHAEMNRQLAAAGASLDAIYYCADAPVSDDRTTVEKLDRKPGPGMLR
                     RAAADLKLDLKASWMIGDLVSDVLAGENAGCRSILLESGQTTPEETMSLAGRFATAKD
                     LPRAAELILD"
BASE COUNT          878 a         1413 c         1409 g          801 t
ORIGIN      
        1 tttcgcgcgc gggcggcggt gaacgaccgc gacaggttca gctccacgac ctccgccccc
       61 aacgaccgcg ccaattcggc gctgccatcc gtcgaattcg aatcgacgta gacgaccggt
      121 aggtcgcgcc ctaccaccga gctcagacac cggcgcagcc gctcgccctc gttgcggccg
      181 atcgcgacaa ttcccatttg actcatgata tctgtttccc ggcgatcagc gcaattcacg
      241 atcgtcaggt cgcttgatcg agagggaacc gttcgagaaa aagttgtaat agaggaagtc
      301 tcgcaacagc gactccgggt cggtgttggg cttgcggaag agccactgcc ggactcggta
      361 taaagagaaa ccgagcgacc aggctaggtc ggcgagcaag gtgcgaatcg gccccagatg
      421 ctttttgttg taataacgcc gggctcggaa ccagtagccg gggagacgtt tgcgttctcg
      481 ttgtctatcc gttacacccg aactttgacc cgcaaggtgg acgacttcgg actgaggcac
      541 gtaccaggcg gcccagcccg cgcggtgagc gcgccggcag aaatccacct cctcgaaata
      601 cataaagtaa ttctcgtcca gcatgccgat ggcctcgaaa acggcgcggc gaatcatgag
      661 gctggcacca gagctccagt cgcagcggct cgcaacgagc ggtgcagggg acgagatgac
      721 ccaccgcgac aagattcggg tcaccaggcc tagccgaacc ccgccttcca actcgcccag
      781 gacggtagga aagcgaaacg ccgaccactc cgcggttccc tctcggttca cgagccggct
      841 gccggcgatt ccgacagcgg gatgatgctc gagaaaatca accagcgccg cgagtgctcc
      901 cggccgtacg agcgtatcgg ggttcagaag ccagacgtag tcaggaggat cggttgactc
      961 gagcgccggg cggatcgccg cgttgttccc gtatgcgaac ccgccgtttc gctcgagggg
     1021 cttgatagag acccagtcac tgaagccctg gtcgcggacg gcggcctgga ggcggggaat
     1081 cgagtcgtcc cccgaagagt tgtcggtaac gacgacccgg acgcgccaat ccgcgaccgc
     1141 ccttacctct gcctccagcg aacgcaggca gtcgatcgtc aagccgggcg tccggtagtt
     1201 gacgatgacg atcaatacat tcagggatga cattcgattc atggcggctg ggacaaatct
     1261 tgttcaattt cggcagtcgt ctccgggtgt cggcaaggac gaacgagctg acgacgcgaa
     1321 accgcgtcgt tctcctcctt cagggggtta tcgagcaacc acggaattga cagcgatcct
     1381 gcgatgattt ggcccgccgg tcgtggcggg attggggaaa agcgcggcga gcttggcggc
     1441 ctcggtgtgc gcgacgtgcc gctcggcaat gcgcgctcgc cctgagcggc ccatctggtc
     1501 gagctcggcg gagcccaatg acaggagatc ggccattgcg tcgatcaacg gtaaaaccgc
     1561 accggcggga accagccatc cagtgacgcc cggttcgacc agctcgggaa tacccgcgat
     1621 gtaagtgctg atcaccggtc tgccgagggc catcgtctcc atgatgacga ccggcagccc
     1681 ctcggcaaag ctaggaagga cgagggcccg cgccgactcg agttggcggc gtacctcctg
     1741 attgctcaag aacccggtga tccgcacgtg cccgcttagc ccacgatcgg tgatttgctg
     1801 ctcgatctcc gctcgcatgg gaccgtcgcc aacaatcacc agctcgaatt catgtccccg
     1861 gtcgcgcagc cctgccgctg cctcaaccag caattgctgg cccttttgct ctgataaccg
     1921 ccccacgttg accagcttcg gctggtctgg cgcgggcaca gcggtggcat tcaagaaaac
     1981 ctcgtccagg ccgcaacgga ccacatggac cttggcccaa tccgccaagt cgcaccagcg
     2041 ataaagctgg ctgcgcgtga actggctgat ggcgacgaca aaggcggcgt gtcgcacctt
     2101 ttcgcgcaag ccgagggcca gaggtgagtc aaattcctca gggccgtgga tcgtgatgct
     2161 ataggcaggg ccgcccaaga ggcgacataa gagcgcggtc accgcggcgt tggttccgaa
     2221 atggacatgc agatgacgag ctccgcaatc aaccagaagc cggcgcagga ggcaggcctc
     2281 ggcaaaatag actagatgca agattagccc tcgatccgac cgacggccca tcctgaccgc
     2341 catcgtcagg gcgatcgtcc atcgccgtgg ctgaaccacg agctggtgca cgagtgccat
     2401 ggcgatcgcg aaggccccga gctccaggag cgcgcaggtc tttttctcct ctgactgatc
     2461 cgcttgatcg gccagcacgc ctgcgaaccg ccgtaacgaa aaccggtgga tcgtgaatcc
     2521 ttgcgcctcg agcgcgacga tctcccggcg gataaacgtc tggctgggca tcggataagt
     2581 gggaatgaaa taagcaatcg tcatcgtatt gcgtcacggt cactggggtg catcaactcg
     2641 ggcgaagccg tgcggatgac tctggtgcca cttccaggca gtccggacaa tctcccgtat
     2701 gtcactccag cgaggcgacc agcccaattc cttgacgatc atgtccggac tggcgatcaa
     2761 caccgcggga tcgccgggcc gtcggccgac aatctcgtgg gcaatcggct gaccgatgat
     2821 ttgctcgcat gctcgcagca cttcgagcac ggtcgcgccg gttccggatc cgaggttgta
     2881 agccctaccc attcccggct cgagcgaaag gacggctcgc tcgtgtgctt gggccaggtc
     2941 cgatgtgtgg acgtaatcgc gcacacagct cccgtcgcgg gtcgggtagt cgttaccgta
     3001 taccaatacc ttgggccggc ggccgacaac cacgtgaaag atgagtggaa tcaggtgggc
     3061 ctcgtgacgg cggtcttcgc cgaaatcgcc gtccggatcg gcgcccgagg cgttaaagta
     3121 ccgcagcagc gtgtaaccta gcccgtaggc gcgggcgtaa tctttgatga gccactcggc
     3181 ggccaacttg gttgtaccat acggcgtctc gggggtttgg ggtgactgct ccacgagcgg
     3241 catctcggca tgaaacccat aggtagccgc ggtgctgctg aagaggatct tccgtacccc
     3301 cgcggtgcgc atagcgtcga gcacactctt ggtgccggcg acgttgaccc ggtagtaggc
     3361 gtcgggatcg ctgatcgagt ctggaacgga tgccaaagcc gcgaaatgca tcaccgcttc
     3421 ggcgccttgg tcgcgtaata cgcgcgccac gcgatcggta tcggcgatat caccgaccac
     3481 cagccgggtg tggccatcgg gtaccgcggc ggcgtttccc tcggacatat tgtcgtatgc
     3541 gatcgggtcg tgcccacggg cgagcagcca gcgtaggcag gcactaccga catagccggc
     3601 gccaccggtc agaaggatct tcatcgcggc gttttcccca tcagtcaaga atcaactcgg
     3661 cggcgcgcgg gaggtctttg gcggtcgcaa atcggccggc aagcgacatt gtctcctccg
     3721 gcgtcgtctg gcccgattcg agcaggatgc tccggcaccc ggcgttctcg ccggcgagaa
     3781 cgtcgctgac gagatcaccg atcatccatg acgctttcag gtctagcttc aaatcggcgg
     3841 ccgcgcgacg caacatcccg ggcccgggct tacggtccaa cttttccacc gtcgttcgat
     3901 cgtcgcttac tggtgcgtca gcacaataat aaatcgcgtc caatgacgcc ccggcggcgg
     3961 cgagctgccg gttcatctcc gcgtggattt cgtggagccc ttcttccgtc aacattcctc
     4021 gagcgattgc cgactggttg gtcacaagca cgcgggcaaa gcccccgcga cggaaacgcc
     4081 gcagggcgtc ggcggctccg gcaatgagcc ggacccgctt ggggtcggcg agatagtgta
     4141 cgtgttcaat cagggtgccg tcgcggtcga gaaaaatggc cggttggagc gtctcggggc
     4201 tgacggtggg cagatcgggg aagttggaac ggacgtcgcg gcgaccgcgc tcgagtgcct
     4261 cgtgagtgcc gatggcgagg tggtatcccc cccacaccca gcccctcatc cgtcccacga
     4321 agtggggaag cacatcgaag cccaagtcga acgcgctcat cgcggcaatc tcgcggtacg
     4381 ccgcggcgtt gacgacgtag aggcccgcgt tggccaggtc gcttttgggc tgctcgggct
     4441 tttccacaaa tgcggtgatc cgacccactt cgtccagctc ggcaatcccg caagcgcgag
     4501 g
//
LOCUS       DATAQL010001120         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_418798, whole genome shotgun sequence.
ACCESSION   DATAQL010001120 DATAQL010000000
VERSION     DATAQL010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418798"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2372)
                     /locus_tag="VHS97_19340"
     CDS             complement(<1..2372)
                     /locus_tag="VHS97_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3450418.1"
                     /translation="MTRLLSFSLVVALLFLLEPVRASADGPSDKFGSTGTFPSDAAGR
                     SLNLGFETGTLADWTVEGTAFASGPVEGDTVSRRRGDMHSGHAGRFWVGSYEKTGDQA
                     MGTLTSVPFRVSKRFASFLVGGGSRAGTRVEIVRKDSSQVIFRESGDDREDLERVVVD
                     LSGHMGREIMIRLVDGEKTGWGHINFDDFRLHDTKPDVPPRRRPTTLDKFVHAGLAPE
                     EAARAMTVPPGFKVTLFAGEPDVVQPIAMAIDDRGRLWIAEAYSYPRRVPDKEAKDRI
                     LILEDIDGDGRFDTRKVFADHLNLVSGLEIGFGGVWVGAAPHFLFIPDRDGDDRPDGP
                     PQILLDGWGQHDTHETPNSFAWGPDGWLYGCHGVFTHSRVGKPGASNAERTPINAGIW
                     RYHPTRHVFEVFAHGTSNPWGIDFDSSGQMIITACVIPHLYHMIQGGRYERQAGQHFN
                     IYTYDDIKTIADHRHYLGANPHGGNGRSDSAGGGHAHSGAMIYQGGSWPAPYAGSLFM
                     NNIHGARLNRDVLTQRGSGFVGSHAPDFLMANDLWSQIISLKYGPDGGVFMIDWYDKN
                     QCHHNDVNAHDRTNGRVFKVSYGDTKASPQDLAKLSSHALVQMQTFPNEWYARHARRI
                     LQERGPNPEVAAQLKALAASTVGQPAIQLRILLAKYAVGGLRYRDDIERGLADKDAVL
                     RAWTIQLVTDQGSPNRSVRAHFAELARTDPSPMVRLYLASAAQRLPLDERWNILEGLV
                     AHTEDIGDHNLPLMYWYAAEPLAAVDKLRAIRLASHSRIPLIREFMARRIG"
BASE COUNT          501 a          767 c          795 g          474 t
ORIGIN      
        1 ccgatccgcc gggccatgaa ctcccggatc aggggaatgc gcgagtgcga cgccaatcgg
       61 atcgcacgca atttgtcgac ggcagccaag ggctccgccg cataccagta catgagtggc
      121 agattgtgat cgccaatatc ctctgtatga gcgaccaggc cctccaagat attccaccgc
      181 tcatcgagcg gcagtcgctg tgccgccgag gccaagtaca gtcggaccat cggcgaaggg
      241 tcggtgcggg caagctcggc gaaatgagcg cgtacagacc ggtttggaga tccttggtcg
      301 gtgaccaact ggatcgtcca ggcgcggagc acggcatcct tgtccgccag cccgcgctcg
      361 atatcatcac gatatctcag cccaccaacg gcatactttg ccaagagaat gcgcagttgt
      421 atcgccggct gccccacagt cgacgccgcc agcgccttga gctgcgccgc gacttctggg
      481 tttgggcctc gctcctggag gatgcggcgc gcgtggcggg cgtaccattc gttgggaaaa
      541 gtctgcatct gaaccagggc atggctactc agcttggcga gatcttgcgg gctggccttc
      601 gtgtcaccat agctcacctt gaagactcgc ccgttggtgc ggtcgtgcgc attcacatca
      661 ttgtggtggc actggttctt gtcgtaccaa tcgatcatga acacaccgcc gtcgggcccg
      721 tatttgagac ttataatttg cgaccagagg tcattcgcca tgagaaaatc cggggcgtgc
      781 gagccgacaa atcccgagcc acgctgagtc aacacgtcgc ggttcagtcg agcaccgtga
      841 atattgttca taaacagcga gcccgcgtac ggggcgggcc aagagcctcc ctggtaaatc
      901 atcgcacccg aatgcgcgtg gccgccgcct gcggagtcgg aacggccgtt gcctccgtgc
      961 gggttggctc ccaggtagtg gcggtgatcg gcgattgtct tgatgtcgtc gtacgtgtat
     1021 atattgaaat gttggcccgc ttgccgttcg taacggcccc cctgaatcat gtggtaaagg
     1081 tgggggatga cgcacgcggt gatgatcatt tggccgctcg agtcgaagtc gattccccag
     1141 ggattgctcg tgccatgggc gaagacctcg aaaacgtggc gcgtcggatg gtagcgccag
     1201 ataccagcgt ttataggagt gcgttcggca ttggacgcac ccggcttgcc gacgcgcgag
     1261 tgggtgaaga caccgtggca accgtacagc caaccgtccg gcccccaggc aaagctgttg
     1321 ggtgtctcgt gggtatcgtg ctggccccag ccatcgagca aaatctgggg cgggccatcg
     1381 gggcgatcgt cgccatcgcg gtctggtatg aacaggaaat gaggggctgc gccgacccaa
     1441 acgcccccga aaccgatttc gaggccgctg accagattga gatggtcggc aaagacctta
     1501 cgcgtgtcga accggccgtc gccgtcgata tcttcgagga taagaatgcg gtccttggcc
     1561 tctttgtcgg gcacgcgtcg tgggtaagag taagcttccg cgatccagag tcggccccgg
     1621 tcatcaatcg ccatggctat cggttgaacg acgtcgggct cgccggcaaa aagagtcact
     1681 ttgaaccccg gcggcaccgt catggcccgc gcggcctctt cgggcgctag gcccgcgtgg
     1741 acaaacttgt cgagcgtggt cggacgcctc cgcggcggaa cgtcgggctt cgtgtcgtga
     1801 agccgaaagt cgtcgaagtt gatgtgcccc cagcctgttt tctctccatc gaccagccgg
     1861 atcatgatct ctcggcccat gtggcccgaa aggtcgacga cgacgcgctc gagatcttcg
     1921 cgatcatcgc cggattcacg gaagatgacc tgactgctgt cttttctgac gatctcgacg
     1981 cgtgtccccg cgcgcgagcc ccctccgacc agaaagctgg caaaccgctt ggacacccgg
     2041 aacggcacgg aagttagggt ccccatggct tggtcgccgg tcttctcgta gcttcccacc
     2101 cagaaccggc cggcatgacc gctatgcata tcaccgcggc gccggctaac cgtgtctccc
     2161 tcgacgggcc cagacgcgaa cgcggttcct tcgaccgtcc agtcggcgag agtgccggtc
     2221 tcgaagccca ggttgagcga acgacccgcc gcgtccgacg ggaatgtgcc cgtcgatcca
     2281 aacttgtcgc tggggccatc ggccgaggcg cgaaccggct cgaggagaaa caaaagtgca
     2341 acaaccaagg agaatgacag caaccgcgtc atggcctttt ccagcctcag aaacagggag
     2401 aggaagacga acccagctcg cactctcgaa accgcgattt cctggtggga cggtcggaaa
     2461 ttccgggtaa aaccaggata cgtattcaaa gcggcgaaag ggagagggcc ggggtgagcg
     2521 ggcgcgcacg gatgcgg
//
LOCUS       DATAQL010001121         3787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2406788, whole genome shotgun sequence.
ACCESSION   DATAQL010001121 DATAQL010000000
VERSION     DATAQL010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406788"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VHS97_19345"
     CDS             <1..341
                     /locus_tag="VHS97_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450419.1"
                     /translation="SRHASTFYRATELPPIPIPDHPLADPGNDKITHAFQKTANTIEA
                     ETDLNGAIYRTIIDYAFGSGDRGLTLVGHDKEGRTLEYRLSHYPDDVGWDITSGQPAQ
                     PDGNDLQYQG"
     gene            372..995
                     /locus_tag="VHS97_19350"
     CDS             372..995
                     /locus_tag="VHS97_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450420.1"
                     /translation="MECHNTNPHAIVAGSNGFAADRAIGCERCHGPGGNHVQVVLSKD
                     FVKKSDADLAIARPSLASGAAIVGLCADCHSARKKGLRLEPGSPQAVRFQGTTLTWSR
                     CYTESDGKLDCLTCHNPHKNAEADPHGYELKCLHCHSPAADTTVLSQSLKVKTEPGRQ
                     SSCPVQPEGGCIDCHMPKREALMAHTRFTDHFIRIPAEPELQSKRQP"
     gene            1349..2611
                     /locus_tag="VHS97_19355"
     CDS             1349..2611
                     /locus_tag="VHS97_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450421.1"
                     /translation="MPRSPLPEPAAPSRKPAPKSWGRTSPWVDDGPVELTDPGQVALD
                     AAGDASGPTDAEIIWEDSPGQFDQTKASERPSRAGVRASPEGEQFWEEDVNRAAAAAR
                     SADTADRRRDNSGADDSPARAPLGQGTAAQSGRTARQSNQRVRRRTADVAVDRGRPVD
                     APAPPGVELRARRKRPSRLSVVLVVVPLLVIATVAWRYRRQRHQEYPVVAERGKIEGI
                     PALEEGNFDKAHQLLSAAKSAVDALGGAVEGADDIRNAADEAAIFVDQSPRSLEEMLG
                     EAGRTDPQLWASKFETLYKGRAILIESHVAAEPEPGRSSSYQIDYVIFPPGGASNFND
                     QREARPARYGLIDLTGFRLFELARPSKGVQVTFGARLASFQYDPQTDVWLIGLEPGSG
                     VIIRHHKALEALGWQHGIEDEAPPETRP"
     gene            2608..>3787
                     /locus_tag="VHS97_19360"
     CDS             2608..>3787
                     /locus_tag="VHS97_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450422.1"
                     /translation="MRYVPWAVAIVCACGITRPATGQRPADAVAVEPGELERRTDLIG
                     RLVVVDDHVKYYVPRTGSNPDELQLKRTPITFQVPRGLRPRGSTPMSAVVVRGVLARE
                     EGRLVCQVSELKPVSGDLERLERGLASLTPNDFETRKAWALWAERRAGDFKDQALLNR
                     ARALEGEALSIESKVKRLGVDAPAEWLAMAQDARRRQVPESDSGALGHRAFRAKLAAA
                     TSEAELKTLIEQIEAFFPGAAADKASGRVNLAQWETPYTDDPAGTYRNRATPPHVRKG
                     FDRRLWADATARLLEIEAAQDLPAAIAASERASSLVPERPNLAANILEKATRLARPDV
                     GSLRLSEVKAVAAAIRERLRQPEEARTVLADWLKIQRDRLSATDAEGPVDLANLYEEL
                     L"
BASE COUNT          738 a         1218 c         1210 g          621 t
ORIGIN      
        1 agagccgcca cgccagcacc ttctatcgcg caaccgagct cccgcccatt cccatccccg
       61 accacccact cgccgatccg ggcaatgata agatcacgca cgcgtttcaa aagacagcga
      121 acacaataga ggcggaaacc gacctgaacg gcgcgattta ccggaccatc atcgattacg
      181 cgtttggctc aggagaccgg gggttgactc ttgtcgggca cgacaaagaa ggccggacgc
      241 tcgaataccg gttatctcac tatcccgatg acgtaggctg ggatatcact tccggccagc
      301 cggcgcagcc cgacggtaac gacttgcaat accagggata gttcctgtcc cgcgatgagt
      361 tgcgtcattg tatggagtgc cataacacca atccgcacgc aatcgtggcc ggttcgaacg
      421 gctttgcagc ggatcgcgcg atcggctgcg aacggtgcca cggtcccggc ggtaatcacg
      481 ttcaagtcgt gttatcaaag gatttcgtca agaaaagtga tgcagacctc gctatcgccc
      541 gtccctcatt ggcatccgga gcggccatcg tcgggttgtg cgccgactgc catagcgcac
      601 gcaaaaaggg gctgcggctc gaaccgggct cgccccaggc ggttcgcttt cagggaacta
      661 ccctgacctg gagccgttgc tacaccgaaa gtgatggcaa gctcgactgc ttgacctgcc
      721 acaacccgca caagaacgcc gaggccgacc cgcatgggta cgagttaaag tgcttgcatt
      781 gccattcgcc cgcggcggac acgaccgtgc tttcccagag tctgaaggtg aaaaccgagc
      841 caggcaggca aagctcttgc cccgtgcaac cggagggtgg ctgcatcgat tgtcacatgc
      901 ccaagcgcga ggccttgatg gcccatactc gtttcactga tcatttcatc cgcatccctg
      961 ccgagcctga gctccaatcc aaaaggcagc cctgagaaag ggcgcgcggg atcgtcagat
     1021 ccgcgaggcg ccatactcga taagttgacc ccgggcgcat gttcttcgat aatttcaccg
     1081 taagtcattt ccggctcctc ggggcgcggc cgcttttccg accatgcgcg ggttgctgcg
     1141 tgggtcgcga ggccaggtgg tcaggatgag gtgatccttg aagtttctcg ggcgggcaag
     1201 aaggttcttt caggagctcg cttcggagcc cgagggaagg gttcaatact tcaatgtgac
     1261 gtgtgggtcc ggccaccgaa ttcgcggtga acgaaccgaa ggctaccagg ccctacgttg
     1321 cccggcctgc gggaatggtg tctttgtgtt gccgcgaagc ccgcttcccg agccggccgc
     1381 gccgtcgcga aagccggcgc ccaaaagttg ggggcggaca agtccttggg ttgacgatgg
     1441 accggtcgag ctgaccgacc cgggccaggt agcgctcgat gccgccggcg acgcgtcggg
     1501 cccgaccgat gccgagatta tctgggaaga ttccccgggg caattcgacc agaccaaagc
     1561 tagcgagcgg ccgtcgcggg cgggagtgag agccagcccc gagggcgagc agttctggga
     1621 ggaagacgtc aatcgcgccg cagcagccgc ccgatcggcc gacaccgccg atcgccggcg
     1681 ggataattcc ggcgccgacg acagccccgc tcgggcgccc ttaggccaag gcaccgctgc
     1741 ccaaagcggc cggaccgcca ggcaaagcaa tcaacgagtc cggcgacgca ccgcggacgt
     1801 cgcagtcgat cgcggccgcc cggtcgacgc gccggcgccg cccggcgtgg aactccgggc
     1861 ccgacggaaa aggccaagcc ggctcagcgt agttcttgtc gtcgtgccct tacttgtgat
     1921 tgccacggtt gcctggcggt accggcgaca gcgccaccag gagtaccccg tcgtcgccga
     1981 gcggggcaag atcgagggaa tccccgcgct tgaggaagga aacttcgaca aggcccatca
     2041 gctcctgtcg gccgccaaat ccgcggtcga cgcgctgggg ggggctgtgg agggcgccga
     2101 tgatatccgc aacgccgccg acgaggccgc gatcttcgtc gatcagagcc cgcgtagcct
     2161 cgaggaaatg cttggtgagg ccggccgaac cgatccccag ctctgggcct ccaaatttga
     2221 gacactctac aaaggtcgcg cgatcttgat cgagtcgcac gtcgcggccg agcccgagcc
     2281 cggacgctcc tcgagctacc agatcgacta cgtgatcttc ccccccggcg gagccagcaa
     2341 tttcaacgac cagagagagg ctcggccggc tcgatacggc ttgattgact tgacgggctt
     2401 tcggttattc gagctcgctc gccccagcaa gggagttcag gtgaccttcg gagcgcgact
     2461 ggcctctttt cagtacgatc cgcaaaccga cgtgtggttg attgggctcg aaccgggcag
     2521 cggcgtcatc atcagacatc acaaggcctt ggaggcactc ggctggcaac atgggatcga
     2581 agacgaggcg cccccggaga cccgaccttg agatatgttc catgggccgt ggcgatcgtc
     2641 tgtgcctgcg gaataacgcg gccggccacg gggcaaaggc cggcggacgc cgtggccgtc
     2701 gaacccggcg agctcgaacg tcggacggac ctgatcggcc gactggtggt tgttgacgac
     2761 cacgtcaaat actacgtccc aaggacgggt tccaatccgg acgagctgca attgaagcgc
     2821 acgccgatca catttcaggt gccgcgcggc cttcgtcccc ggggctcaac cccaatgagt
     2881 gccgtggtgg tgcgcggcgt gctggcgcgc gaggaaggcc ggcttgtctg ccaggtgagc
     2941 gagctcaagc ccgtcagcgg cgaccttgaa cggctcgaac gagggctcgc gagcttgacc
     3001 cccaatgatt tcgagacgcg caaggcctgg gcgctctggg ctgagcggag ggcaggggac
     3061 ttcaaggatc aagcgctctt gaaccgcgcc agggcactcg agggtgaagc cctgagtatc
     3121 gagtcgaaag tcaagcgtct gggagtggac gccccggccg aatggctggc catggctcag
     3181 gacgcgcgcc ggcgtcaggt tcccgagtcc gactccggcg cactgggcca ccgcgcgttt
     3241 cgagccaagc tcgccgccgc tacgagcgag gccgagctca agacgctcat cgagcagatc
     3301 gaggcctttt ttccgggtgc ggccgccgat aaagcgtcgg gccgtgtcaa cctggcgcag
     3361 tgggaaacgc cgtacaccga cgatccggcg ggcacctatc gcaatcgcgc aaccccgccc
     3421 cacgtcagaa agggcttcga tcggcggctc tgggccgacg ccaccgctcg cttgctcgag
     3481 atcgaggccg cgcaggacct gccggcagcg atcgctgcct cggaacgtgc ttccagcttg
     3541 gtccccgaga ggcccaacct cgcggccaac atactcgaaa aagcaacacg cttggcccgc
     3601 ccggacgtag ggtctcttcg attgagcgaa gtcaaggctg tcgccgcggc cattcgcgaa
     3661 cggctacgcc aacccgagga agcgagaacc gtgctggccg actggctcaa gatccagcgc
     3721 gatcgcttga gcgcgaccga cgccgaaggc cccgtcgacc tggccaatct ctacgaagag
     3781 ctgctgg
//
LOCUS       DATAQL010001122         5602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3627101, whole genome shotgun sequence.
ACCESSION   DATAQL010001122 DATAQL010000000
VERSION     DATAQL010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3627101"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VHS97_19365"
     CDS             complement(<1..1073)
                     /locus_tag="VHS97_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX3450423.1"
                     /translation="MPLPPLWALGFANPALLYALAAAGVPIVIHLLNRRKYREVPWAA
                     MRFLLAALRKNRRRLRVEQWLLLAIRTLVVLLVVAAVARPFLESFGAVMPGSRTHRVI
                     VIDDSLSMGYTAGESSRFAQAKAVAAQLVKDSHRGDAISLILMGSPPRVVIADPTQNL
                     SVVKKEIDELTLSDGGTDLAMTLEKINNVLEISPNAQKEVVFLTDLQTASWRPPVESA
                     DKIKRALVKLETYRPRSVVIDLGKSGGDNRAVTDLKLEIPVVTVGAPVLIRATLRNFG
                     ANRADGVRARLTADGRLSPEQPFDLPTAEDIQAVFLQQFDTPGDHLVEVSIDDDPLAI
                     DNHRWLVVPVRESLNVLLVDGHFR"
     gene            complement(1112..2011)
                     /locus_tag="VHS97_19370"
     CDS             complement(1112..2011)
                     /locus_tag="VHS97_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3450424.1"
                     /translation="MENYQKYLDPHTLASLEGLDLQARMVVEGYVAGMHPSPYHGFSV
                     EFAEHREYVPGDDIRHVDWKVWSKTDKLYLKQYEEETNLLTYLLLDTSESMAYASGDN
                     VTKLQYAQIIAASLSYLILQQQDSVGLVVYDDAVRRYLKPAGQTSQLKEILRLLDVTP
                     AREKSDLGLVFNDLAERFKKRGVVVVLSDLFDDVSRIITGLKHFRHRRHEVIVFHVLD
                     PAELDFPFRDTTLFKGLEGMPEILTDPHALKRAYQVELGLFLEELEKGCRMVDIDYVP
                     LRTDQSLDVALSSYLASRSARIR"
     gene            complement(1968..3050)
                     /locus_tag="VHS97_19375"
     CDS             complement(1968..3050)
                     /locus_tag="VHS97_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3450425.1"
                     /translation="MSDANPTETLQSEDDLAAVERLKDAFTKLKAEMAKVIVGQHAVL
                     EELLIAIFARGHCLLIGVPGLAKTLMIHTMADALNLSYNRIQFTPDLMPSDITGTEVI
                     QEDKATGVRQFKFLRGPVFANIVLADEINRTPPKTQAALLEAMQERQVTVGGERHRLP
                     DPFFVLATQNPIEQEGTYPLPEAQLDRFMLNILVGYPSEEEELDIVRMTTSVQRPAVS
                     KVLSGTDILALQELVRKVPIADHVSRYAVRITRHTRRDKDSSLNFIRDYVSWGAGPRA
                     SQYLVLAAKARAVLHGRYYVSTDDIRSVAGPVLRHRIITNFNAEAEGLKPDDLVERLL
                     KAVPVDENEAEQSGKLPKIFRSAHVG"
     gene            complement(3157..>5602)
                     /locus_tag="VHS97_19380"
     CDS             complement(3157..>5602)
                     /locus_tag="VHS97_19380"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3450426.1"
                     /translation="DLIYWPTSGEIEVLDQRTALRAEPPIKLEATYHTKGGNLVAGDG
                     YLIVAQADGMVVFCQNSRLIERYQNDIALAPERASNYFRLARAAEAIGRDQLALEMYE
                     QAARRAKSGETIDGITLVGAARDHRFRLLFRLAGLARKARRWDDASAYLDGAATVARS
                     DAERLQAQLLRADVLLDAARPREAVDICERLLNDDRLRPLAITAADGHRTIRADLFIA
                     DRLNSIVRDHGRQVYATYDQEAVRLFERGKAEKDVRVLDELCRSFPVARVVPDALVVL
                     GSLQESSRRLPEAAHTYKRLLSLAPDDDRRALAIWRLAHVYEERKLLVAARDSYLDLQ
                     ARFPDIHLNGARDQATYAELVAAELARPAYAQLIADRPVPPTPVPLVRRWQWQAPSSQ
                     AIRVIYADGVAPSLDAGRLFVVDKTALRLVDPATGAVRWTSELAQTAVWVGYLADKLI
                     VATPHQIIALELGQGTVQWRYGQSPKGSDQDRPDPFAEVKEGDDPQERGRRGEAFSDF
                     QLVRGHVFCLRGTRELISLDGDTGALDWSFSSPPGQINPNFWIGTDRTVLQVDKPNQL
                     LVLRSDDGQPISRTPLAESERLERPPMPADEDSVILVSDRRTVKKFDFNHCQTVWVYQ
                     ESPDLPINGPPRLIGDSERLLVLHDGRLLIRLDPATGSKRWSCLLGIEDLSERPTAMA
                     YDDKRFYCVNLENILGGLRQVVRAVALEDGSRVWSAHLSGPGEASWSLALTQRYVIAY
                     PRNKTNASMEIENMPVIVRLRDTGTLVQRFVFPTTIADVTVRADSRGALVATGRGLWG
                     LGSKEASSAPPSERLR"
BASE COUNT         1083 a         1700 c         1780 g         1039 t
ORIGIN      
        1 cggaaatggc cgtcgaccaa gaggacattc agcgactccc gcaccggcac gaccagccac
       61 cggtgattgt cgatcgccag ggggtcgtcg tcgatcgaga cctcgaccag atggtcccca
      121 ggtgtgtcga attgctgaag gaagacggcc tggatgtcct cggcggtggg aaggtcgaag
      181 ggctgttcgg ggctcaggcg tccgtcggct gtaagccgcg cgcggactcc gtcggcgcgg
      241 ttggcaccaa agttacgcag ggtggcacgg atcaggaccg gcgcacccac agtcaccacg
      301 gggatctcca gtttgaggtc ggtcaccgcg cggttgtctc cccccgattt tccgaggtcg
      361 atcacgaccg aacgtggccg gtacgtttcg agcttgacca gtgcgcgctt gattttgtcg
      421 gcggattcaa ccggcggccg ccagctcgca gtctggaggt cggtcagaaa gacgacctct
      481 ttttgggcat tcggcgaaat ctcgaggacg ttgttgatct tctccaacgt catagccaaa
      541 tcggttccgc cgtccgagag cgtcagctca tcgatttctt tcttgacgac gctcaggttt
      601 tgggtcgggt cggcgatcac gacgcgcggg ggcgatccca tcaagatcag gctgatggcg
      661 tcgccgcggt gcgaatcctt cacgagctgg gcggccacag ctttggcctg agcaaagcgg
      721 ctggattcgc cagcggtata gcccatagag agcgaatcgt cgatcacgat gactcgatga
      781 gtccggctgc ccggcatgac agcgccaaac gactccaaga atggtctggc cacggccgcg
      841 acgaccaaga gcaccaccag cgtccggatc gccagcaaga gccactgctc gacccgaagg
      901 cggcggcgat tcttgcgaag tgcggccaag agaaaccgca tcgccgccca cggtacctcg
      961 cggtacttgc gccgattgag cagatggatc acgatcggca cacctgccgc ggccagcgcg
     1021 tagaggagcg cggggttggc gaatcccagg gcccagagtg gcggcagcgg caaggggggc
     1081 aactccttcg aaaacgacag gctggggcgc gttagcgaat tcgggccgat cgcgacgcca
     1141 agtagctcga cagcgcgaca tcgaggctct gatcggtgcg caggggaacg tagtcgatgt
     1201 cgaccatccg acaccctttc tccagctctt ctaagaacaa gcccagctcg acttggtagg
     1261 ctcgcttgag cgcgtgcggg tcggtcaaga tttccggcat cccctcaagg cccttgaaaa
     1321 gcgttgtgtc ccggaacgga aaatcgagct cggccgggtc gagaacgtga aacacgatca
     1381 cttcgtggcg tcgatgccga aaatgcttga gcccggtgat aatccgcgac acgtcatcga
     1441 acagatcaga caagacgacc acgaccccac gcttcttgaa ccgctccgcc aggtcgttaa
     1501 acaccagccc caggtccgac ttttcgcggg cgggagtgac gtcgagcaaa cgcaagatct
     1561 ctttgagctg cgacgtttgc ccggccggct tcaagtaacg gcggaccgcg tcatcgtaga
     1621 cgaccaggcc caccgagtct tgctgctgga gaatcagata cgacagcgaa gccgcgataa
     1681 tttgggcgta ctggagcttg gtaacgttgt cgcccgaggc ataggccatc gactcgctcg
     1741 tgtccaggag caagtacgtc agaaggttgg tctcttcttc atactgcttc aagtaaagct
     1801 tgtcggtctt ggaccagacc ttccagtcga cgtggcggat gtcgtcgccg ggcacatact
     1861 cacgatgctc ggcgaactcg acggagaagc cgtggtacgg gctgggatgc atcccggcca
     1921 cgtagccctc gaccaccatg cgcgcctgca ggtccagccc ctcgaggcta gccaacgtgt
     1981 gcggatctaa atatttttgg tagttttcca ctttgctccg cctcgttttc gtcgacgggg
     2041 accgccttca acagccgctc gaccagatcg tcgggcttaa ggccctcggc ttcggcattg
     2101 aaattggtga tgatccgatg ccgcaagacg gggccggcga cggagcggat atcgtcggtc
     2161 gagacgtaat accggccgtg caagaccgcg cgggccttgg ccgccaggac aaggtactgg
     2221 ctggcgcggg ggccggcgcc ccagctcacg tagtcgcgga tgaagttcag gctgctgtcc
     2281 ttgtcgcggc gggtgtgacg ggtgatccgc accgcgtacc gcgacacgtg gtcggcgatc
     2341 ggcaccttgc gcacgagctc ttgaagcgcc aggatgtctg tccccgacaa aaccttggag
     2401 acggccggcc gctggaccga agtcgtcatc cgaacgatat cgagctcttc ttcctccgat
     2461 gggtaaccga ccaagatatt gagcatgaac cggtcaagct gcgcttcggg gagtggatac
     2521 gtcccttcct gctcgatagg gttttgggtg gccagaacga agaaggggtc cggcagccgg
     2581 tgccgctcgc cccccactgt gacttgccgc tcctgcattg cctccaagag cgcggcctgt
     2641 gtcttgggag gagtccggtt gatttcgtcg gcaagaacga tattggcaaa gaccggccct
     2701 cttaagaact tgaactgccg gacgccggtc gccttgtctt cctggatgac ttcggtgccg
     2761 gtgatgtccg acggcatcag gtcgggagtg aactggatcc ggttgtagct caggttgagg
     2821 gcatcggcca tggtatggat catcagggtc ttggccagcc ccggaacgcc gatcaggagg
     2881 cagtgaccgc gggcgaagat cgcgatcaag agctcttcca aaacggcgtg ctgaccgacg
     2941 atgaccttgg ccatctcggc cttgagcttg gtgaatgcat ccttcaagcg ctcgacggcg
     3001 gcgagatcat cctcgctttg aagggtttcg gtcgggtttg cgtcgctcac gcgtgcctcc
     3061 cggttgctgg taagagtctc gactggtgaa gaatcaagtc gactggtgaa gaatcaggaa
     3121 tgttcacgaa cgttgtaggc ggatctccgt gccccgctag cgtaatcgct ccgagggcgg
     3181 agcggagctc gcctccttcg agcctaatcc ccagaggccg cggcccgtcg ccaccaaggc
     3241 gccgcgcgaa tcggccctta ccgtgacgtc ggcaatcgtt gtgggaaaca cgaaccgctg
     3301 cacgagcgtg ccggtatcgc gcaggcggac gatcacgggc atgttctcga tttccataga
     3361 tgcattggtc ttgttacgtg gataggcaat gacgtagcgc tgcgtcagtg ccaaagacca
     3421 tgacgcctcg ccggggccgc tcaggtgagc ggaccaaacg cgcgagccgt cttccagagc
     3481 aacggcccgg accacttgcc tcaagccacc gagaatgttc tccaggttga cacagtaaaa
     3541 gcgtttgtca tcgtaggcca tcgcggtggg gcgttcgctc aggtcttcga tccccaaaag
     3601 acacgaccat cgtttcgacc cggtcgccgg gtcgagccga atcaataatc gcccgtcgtg
     3661 caataccagc aaccgctcgg aatcgccgat caggcggggg ggaccgttga tgggaagatc
     3721 ggggctttcc tggtaaaccc acacggtctg gcagtgattg aaatcgaatt ttttgaccgt
     3781 ccggcgatcg gagaccagga tgaccgagtc ttcatcggcg ggcattggcg ggcgttccag
     3841 ccgctcgctc tcggccaacg gggtgcggga aatcggttga ccgtcgtcgc tgcgcaggac
     3901 cagcaattgg ttgggcttgt caacctgcag aacggtacga tcagtgccga tccagaaatt
     3961 tgggttgatc tggccgggcg gggaagaaaa cgaccagtcg agggcgccgg tatcgccgtc
     4021 taacgaaatt agctcgcggg tgcctcgcag gcagaagacg tgcccccgca cgagctgaaa
     4081 gtcggaaaat gcctcaccgc gccggccgcg ttcctggggg tcgtctcctt cctttacttc
     4141 tgcgaacggg tcggggcggt cctgatcgct gcccttggga gactgtccat agcgccactg
     4201 cacggttcct tggcccaact cgagcgcgat aatctggtga ggcgtcgcca cgatcaattt
     4261 gtccgccagg taaccgaccc agacagccgt ctgtgccagc tcggacgtcc agcgcaccgc
     4321 accggtcgct gggtcgacca accgcagcgc ggttttgtcg accacgaaaa gccgcccggc
     4381 atccagcgac ggagccactc cgtcggcgta gattacgcgg atcgcctgac tcgatggtgc
     4441 ctgccactgc cagcggcgaa ccaagggaac cggtgtcggc ggcaccggtc ggtcggcaat
     4501 caattgggcg taagcgggcc gagcgagctc ggctgcgacc agctcggcat aggtcgcctg
     4561 gtcgcgtgcc ccgttcaaat ggatgtcggg aaatcgtgcc tgaagatcga ggtaactatc
     4621 ccgcgctgcc acgagcagct ttcgttcttc gtagacatgc gcgagtcgcc agatcgccag
     4681 agcccggcga tcgtcgtcgg gcgcgagact gagcagccgc ttgtaagtat gcgcggcctc
     4741 cggcaatcgc cgtgatgatt cctgcagcga ccctagcacc accaaggcgt cgggaacgac
     4801 gcgggccacg gggaaactgc ggcagagctc gtcgagtacc cggacgtctt tctccgcctt
     4861 gccgcgctcg aagagccgga cggcttcctg gtcataagtc gcgtatactt ggcggccatg
     4921 gtcgcgcaca atcgaattca atcgatcggc gatgaaaaga tcggcgcgga tggtgcgatg
     4981 tccatcggcg gcggtgatgg caagaggccg aagccggtca tcgttcaaga gacgctcgca
     5041 gatgtcgacg gcctcgcggg gacgggccgc gtcgagcagg acatccgcac gcaaaagctg
     5101 tgcttgcagc cgctcggcat cggaccgggc aaccgtggct gcgccgtcta agtaggccga
     5161 cgcgtcgtcc cagcggcgag ccttgcgagc caacccggcc agccgaaaga gcaggcggaa
     5221 ccgatggtcg cgtgccgccc cgaccagggt tatcccgtcg atagtttccc cggacttagc
     5281 cctccgagcg gcctgttcat acatttcgag agcaagctga tcacggccga tggcctcggc
     5341 cgcgcgggca agccgaaagt aattggacgc gcgctcgggg gccagcgcaa tatcgttctg
     5401 atagcgctcg atcagccggc tgttctgaca gaagacgacc atcccatcgg cctgcgcgac
     5461 aatcaggtag ccgtcccccg cgaccaggtt gccccccttg gtatgatagg tcgcctccaa
     5521 cttgatcggt ggctcggcac gcaacgccgt ccgttgatcg aggacctcga tctcgcccga
     5581 ggtcggccag taaatcaagt ca
//
LOCUS       DATAQL010001123         5268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4387082, whole genome shotgun sequence.
ACCESSION   DATAQL010001123 DATAQL010000000
VERSION     DATAQL010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4387082"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1354)
                     /locus_tag="VHS97_19385"
     CDS             complement(257..1354)
                     /locus_tag="VHS97_19385"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3450427.1"
                     /translation="MRDDAAHTEAVFGLFDVASRGWSNVCNFFARWNRRCIGLLLALA
                     ILIPWTPFSRSMPTMKPSYFARGTSPVSIVSFAFGADGQTIATTDESGRAILWRAAEG
                     WAPSPAFKFPGRAQVVAISSDCHFLAIGGDRPHLAVWDPRRASRECSLRIPARSPSNL
                     KLSPDGQTLAISSYDSPEIVLWDVVADHERQTLKGHSAPVLHLAFAPDGRSLASATGT
                     IADSRILIWNLATGRPERHIALPSAAQAMAYSPDSRLVASASPHEKSVRIWAARTGGP
                     AQMIAGHSQSTRSVAFSPDGKLLATGAGDGTAGLWSVVTGREIRRLDGQADVLRNVAF
                     SPDGKTLAATANDGDIRFWDIDEVIANVLDD"
     gene            1613..2206
                     /locus_tag="VHS97_19390"
     CDS             1613..2206
                     /locus_tag="VHS97_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3450428.1"
                     /translation="MLSPSHSITRCIDMLKRGDHAAAEALWDSYVHRLVALARARLGG
                     APRRAADEEDVALSAFDSFCRRAEYGQFARLTDRDDLWQLLVLITERKAINLMRREGR
                     KSRGAGRVVAFSDVEGQRPGEIVDPGPTPEFAAQVVDEFRELLRRLGDDSLRCVALAK
                     MEGYTNQQIADRLGCIEQTVERKLRSIRRIWSAGSET"
     gene            2203..4737
                     /locus_tag="VHS97_19395"
     CDS             2203..4737
                     /locus_tag="VHS97_19395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3450429.1"
                     /translation="MSTSTAAARDNSSVLTVRALERTCDRFEASWKSKARPSIEDYVS
                     DTPEPNRPRLLLELVALDVVYRRRIGERPTPEEYRDRFPSESAAIEGAFDASGAAHGG
                     PVGDAVTVGVPDGSVPTIPGYTVLEELGRGGMGVVFKATAEQLNRFVALKMILSGDLA
                     STEAGARFLKEAEAVARLQHPQVVQIFRIGDYQGRPYLEMEYVEGGSLADRLDGRPWN
                     PTEAARLIESLALAVDHAHIREIVHRDLKPANILLTADGIPKVTDFGLAKSLGADIGL
                     TRTDSIIGSPSYMAPEQARGTASQVGPATDVYSLGAILYELLTGRPPFRAATVLDTLE
                     QVRSADAAPPSRIQPGLPIDLETIVLKCLEKEPTRRYLSAEALATEIGRFRAGEPIKA
                     RPVGFVGRGVKLARRRPLTAALTILGAASIVLLIIMLAKSNLAISRQQRATFEALTRE
                     RWLREELGRANERLAEQQRQTQAALENKTVALEATSEDLQRERQASYFQRIALADSER
                     LAGRYDRVDRVLAGCPDDVRGWEWRYLKQSSRREPLAFIGHTGEVWDAAISPDGLRLA
                     SAGFDHMINLWDVATGKLERTLRGHKERAYSVGFDKDGTHLVSASADKTAIIWDVASG
                     KALHVLRGHTDNVRCAAFSFDGHTVVTGSWDGDFRVWNTGAGEMYRKYETGAGWITRV
                     AFSPDSRYVAVGGSSGRAEIREVYSGRVAQAFESKLGPILSVAFSPDGWRIATTNNAA
                     GIGVVKIWDVLRGHEILSFKVSSGLIERVAFSPDGRRLATSGWDGVVRILDVATGHEV
                     LALRGHSDRVWGVNFSPRGDALVSASADRKILLWNASHQDRGPTRE"
     gene            4900..>5268
                     /locus_tag="VHS97_19400"
     CDS             4900..>5268
                     /locus_tag="VHS97_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX3450430.1"
                     /translation="MQKNFVLTVTGPDRIGVVERVTGLLVNEGGNVETSRMARLGGEF
                     AILMLVSMPEDRIAALDADLLALGAEGYKVTTTATDRARAGARADWRPYRIEVEGADD
                     EGIIHEVARYLSEREINIESV"
BASE COUNT          992 a         1666 c         1712 g          898 t
ORIGIN      
        1 tgacgggcgc tggcatacta ccttgccgcg cccgaacctc gcgcgaaggt ccgctgtgcg
       61 cgccggggtt gctccaacgg ccgccgtcat cacactcgac ggcacttgac tttcaatggt
      121 acatctgtct ccactcgtgg cccgatccca gcccaatcga tcccagaata agacgcagcc
      181 cgcgacgacc gtggctgcca cgatcacggc cgccacccga tcccgataca tcgtgctcac
      241 cgtcacactc tatgcgtcaa tcatcaagca cgttcgcgat cacctcgtcg atgtcccaaa
      301 agcggatgtc gccatcattc gcggtcgcgg ccagtgtctt cccgtcaggc gagaacgcaa
      361 cgttgcggag cacatcggcc tgaccgtcga ggcgccgaat ctcgcgcccg gtcacgacac
      421 tccagaggcc tgcggtccca tcgccggccc ccgtcgccag cagctttcca tccggtgaga
      481 acgccaccga acgtgtggat tgggaatgac cagcgatcat ctgcgccggg ccgccggtcc
      541 gggcggccca gatccgaacc gacttttcat ggggggacgc gctcgcaacc aggcggctat
      601 ccggtgagta cgccatcgcc tgagccgccg agggcagcgc gatgtgccgc tccggccgcc
      661 cggtggcgag gttccagatg aggatccgcg aatcggcgat cgttcccgtg gccgaggcga
      721 gcgaccggcc atcgggtgcg aaagccaggt gcagcaccgg ggccgaatgc cctttcaggg
      781 tctgccgttc gtgatcggcg acgacgtccc agaggacgat ttcgggggaa tcgtagcttg
      841 agattgccag agtctgcccg tcgggcgaca gtttcaagtt actgggcgag cgggcgggta
      901 tccgcagaga acactcacga ctcgctcgtc tcggatccca tacggcaaga tgcggccgat
      961 ccccaccgat ggcaagaaaa tgacaatcgg aggagatggc cacgacctgc gcgcgaccgg
     1021 gaaatttgaa cgcgggtgag ggagcccaac cctcagcggc ccgccaaaga atcgcgcgcc
     1081 ccgactcatc cgtcgtcgcg atcgtctggc cgtcggcgcc gaacgcgaag ctcacgatcg
     1141 agaccggcga agtgccgcga gcgaaatatg acggcttcat tgtcggcatc gatcgggaaa
     1201 acggagtcca ggggatgagg atcgccagcg ccaacaggag gccgatgcac cttcggttcc
     1261 aacgtgcgaa aaagttacac acgttgctcc accctcgcga cgcgacgtcg aatagaccga
     1321 aaaccgcctc agtatgggcc gcgtcgtctc gcatcgatca actcctcatc gcccagcagg
     1381 acttgcaccc actgaacggc cagaccttcg tggctcgcga tcaacgtcga acaggcgtct
     1441 ccccggagat taactcgcca acggctccaa taacccagcg cgtgtctcga aaaacttcgt
     1501 gccgctcatc gtgagggttg gaccctcggc gcggggttta cctcccttcg agccggccct
     1561 aagataaaaa cgcgaacact tcggattgcc tttaaacgga gcagcgtccc gcatgttgtc
     1621 gccgtctcat tcgattaccc gctgcatcga catgctcaag cgtggcgacc atgcggcggc
     1681 tgaggcgttg tgggattcgt atgtccaccg acttgtagcg ctagctcgcg cgcggctcgg
     1741 cggcgcgccg cgacgcgcgg ccgacgagga agacgtggca ctctccgcat tcgacagctt
     1801 ttgccgccgg gccgagtacg ggcagttcgc gaggcttacc gaccgtgacg acctctggca
     1861 actcctcgtc ctgatcaccg agcgaaaagc gatcaacctg atgcgccgcg aggggagaaa
     1921 atcgcgtggc gcgggccggg ttgtcgcctt ctccgacgtc gaaggccagc gccctggtga
     1981 gatcgtcgac cccggtccga cccccgagtt cgcggcgcag gtcgttgacg agttccgcga
     2041 gctcttgcgg cgactcgggg acgactcgct ccggtgtgtc gctctcgcga agatggaagg
     2101 ctacaccaat cagcagatag cggaccggct cggttgcatc gagcagacgg tcgagcggaa
     2161 actgcgctcg atccgccgga tctggtccgc ggggagtgag acgtgagtac ctcaaccgcc
     2221 gccgcgcgcg acaattcatc agtcctgacc gttcgcgccc tcgagcggac ctgcgaccga
     2281 ttcgaggcgt cgtggaagtc gaaggcgcgg ccgtcgatcg aggattacgt gagcgacacc
     2341 ccggagccta atcgcccgag gcttttgctg gagttagtcg cgctcgatgt cgtctacagg
     2401 cgccgcatcg gcgagcgacc gactcccgag gagtatcgcg accggttccc gagcgaatcg
     2461 gccgcaatcg agggcgcgtt cgacgccagc ggggccgcgc atggcgggcc ggtgggcgac
     2521 gcggtgacag tcggcgtgcc ggacgggtct gtcccgacca tcccagggta cacggtgctc
     2581 gaggaactag ggcgcggggg catgggggtc gtcttcaagg ccacggccga gcagctcaac
     2641 cggttcgtcg cgctcaagat gatcctttcg ggcgatctgg cgagcaccga ggcaggagcg
     2701 cggttcctca aggaagccga ggctgtcgca cggctccagc acccacaggt agtccagatc
     2761 ttccggatcg gcgactacca aggccggcct tatctcgaaa tggagtatgt agagggaggg
     2821 agcctggcgg atcggctcga cggccggccg tggaatccca ccgaagcagc gcggctgatc
     2881 gagtcgctcg ccctggccgt cgaccacgcc catatccggg agatcgtcca ccgcgatctg
     2941 aagccggcca acatcttgct gacggctgac ggcattccga aggtcaccga cttcggcctg
     3001 gccaagagcc tgggggccga catcgggttg acgcgaaccg actcaatcat cggctcaccg
     3061 agctatatgg ccccggaaca agccagggga acggccagtc aggtcgggcc ggcgaccgat
     3121 gtctactcgc tcggtgcgat attgtatgag ctgttgaccg gacgacctcc gttccgagcg
     3181 gccacggtcc tcgacacgct cgaacaggtc cgctcggccg acgccgcgcc cccatcgcgg
     3241 atccaaccgg gattgccgat cgatctcgag acaatcgttt tgaagtgcct cgaaaaggaa
     3301 cccacgagaa gatatctcag cgcggaggca ctggccaccg aaattggccg ttttcgcgcc
     3361 ggcgaaccca tcaaggcgcg cccggtgggt ttcgtcggtc gaggggtaaa gctagcgcgc
     3421 cgccggccgc tgactgcggc tctgacgatt cttggtgcgg catctatcgt tctcttgatt
     3481 attatgctcg cgaagagcaa ccttgccatc agccgacagc agcgtgcgac gtttgaagct
     3541 ctcacacgcg agcgctggct cagggaggag ctcgggcgcg caaacgagcg cttggccgag
     3601 caacagcgac agactcaggc agcgcttgag aacaagaccg tggcgctcga ggcgacgagc
     3661 gaagaccttc agcgcgagcg ccaggcatcc tacttccagc ggatcgccct ggccgattcc
     3721 gagaggttgg ccgggcggta tgaccgcgtc gaccgggttc tggcgggctg tcctgatgac
     3781 gttcgcggct gggagtggcg ttacttgaaa cagtcgtcac ggcgcgagcc gctggccttt
     3841 atcggccaca ctggagaagt ctgggacgcc gccatcagcc cagacggcct ccggctcgcg
     3901 tcggcgggct tcgaccatat gatcaacctt tgggatgttg ccaccggtaa gctcgagcga
     3961 acccttcgcg gccacaaaga gcgggcatac agtgtagggt tcgacaagga cggaacacac
     4021 ctggtctcgg ccagcgccga taagactgcg atcatttggg atgtggcgag cggcaaagcg
     4081 cttcatgtct tgcggggcca cacggataac gtccgctgcg cggcgttcag cttcgacggg
     4141 cacaccgtcg tcacggggag ttgggatggg gatttccgcg tgtggaatac gggtgccggc
     4201 gaaatgtatc ggaagtacga aaccggcgcg ggatggatta ctcgcgtcgc gttcagcccc
     4261 gactcccgtt acgtcgccgt cggcggttcg tcggggcggg ccgagatccg cgaagtctat
     4321 tccggccgcg ttgctcaggc gttcgagagt aaattggggc ccattttgag tgtcgccttc
     4381 agccccgacg gctggcgaat cgcaacgacg aacaacgccg ccgggatcgg cgtcgtcaag
     4441 atctgggacg tgttgagagg gcacgaaatc ctctcgttca aggtcagctc ggggctaatc
     4501 gaacgcgtcg cattcagccc tgacggccgc cggctggcga cctcgggctg ggacggcgtc
     4561 gtcaggatct tggacgtcgc caccggccat gaggtcctcg ctttgcgcgg tcatagcgac
     4621 cgagtctggg gggtcaactt cagcccccgc ggcgatgccc tcgtttcggc cagcgccgat
     4681 agaaagatct tgctctggaa cgcaagccac caggatcgcg gcccaacccg agaataatag
     4741 gatcggcgtc atggccagaa tggaaccggg gtccgcgcca gcggtaagat atcggttcgg
     4801 acggcaatcg ggcgcgacgt cgggtcatcg ccaggaccgt gcgatggact gatcggttgc
     4861 atcgtttcgt agactactcc ggacgggggg aatcgctcca tgcagaagaa ttttgtgctg
     4921 accgtgactg gaccagaccg tatcggagtc gtggagcgcg tgactgggct gttagttaat
     4981 gaaggtggaa acgtcgagac gagccggatg gcccgcctcg ggggcgagtt cgccatcttg
     5041 atgctcgtgt cgatgcctga ggaccgtatt gccgcgctcg atgccgacct gctcgccctc
     5101 ggcgccgaag gctataaggt gaccacgacg gcaacggacc gcgcccgcgc cggtgcgcgc
     5161 gccgattggc ggccctaccg gatcgaggtc gagggagccg acgacgaagg gatcatccac
     5221 gaggtcgccc gctacctgtc ggaacgagag atcaatatcg agtctgtc
//
LOCUS       DATAQL010001124         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1031925, whole genome shotgun sequence.
ACCESSION   DATAQL010001124 DATAQL010000000
VERSION     DATAQL010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031925"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VHS97_19405"
     CDS             <1..349
                     /locus_tag="VHS97_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450431.1"
                     /translation="RESTTPRPVAFFAPDRQGIAPLPVFEQYDAKNGQTLSVAVNGQA
                     PDGSDAHPLFFLLPADLKDYTAATVPFYEYREEGSGRRVYSVEAPSAVARSHWTPVLL
                     GRVWRNPARMRLW"
     gene            411..1664
                     /locus_tag="VHS97_19410"
     CDS             411..1664
                     /locus_tag="VHS97_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450432.1"
                     /translation="MKSSPPQPDDTPTPDCDMPDKRITPGDRLTLSRERSWLEEIALQ
                     LENNPDECWQAIESLAQVQHELRMSIIDELSVLGPRPGAAKLLRLLSSTRDPATRSAA
                     QSALERIVWHADSIPKLMASSTLAVVTARTGVEKALGYGRPCLSLAGRNGSGPRLARC
                     LVTPVDGRGRATIVVSVNQMHQRRTAAFLCDVERGIRDVVGEVEPDSDHAGGLIDAFD
                     QQPAGDCARDVPELALLLLAGSLMLTDVNATHAVGEWLLGTLGPDFRPAELPTVISDL
                     NASSIDHEEMPQRALSILEACPSWLDVSPLTFELAREIWLREGRDAADPTRDAGAYRY
                     LFEHRLSRRLELYGRMLLWMAWLWKFSGLADLSLSAATMAAQLSDEQYAVPSHPFTVE
                     LTTRSLLAAQARMRTADDPRKEHRA"
     gene            complement(1792..1920)
                     /locus_tag="VHS97_19415"
     CDS             complement(1792..1920)
                     /locus_tag="VHS97_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450433.1"
                     /translation="MGDARDTAKKKKADEKKKASKPDAKSAAPAAKASAAAKPTKK"
     gene            complement(2199..2456)
                     /locus_tag="VHS97_19420"
     CDS             complement(2199..2456)
                     /locus_tag="VHS97_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450434.1"
                     /translation="MNSLKSMDSTKPSDLPVLGTQRSPCCHLRSAGMYVFTDGPREET
                     ADNYDSSSYWCSETMKSFGPDDEMAGGRECRDPARSCYVPL"
     gene            complement(2453..>2638)
                     /locus_tag="VHS97_19425"
     CDS             complement(2453..>2638)
                     /locus_tag="VHS97_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dehydrogenase"
                     /protein_id="HEX3450435.1"
                     /translation="TAAEKVITGKRVTIETAAAAGEAAAEGAVPLSMNGYKVPLTKTV
                     VKRALLAAVGNRYWEEA"
BASE COUNT          475 a          804 c          809 g          550 t
ORIGIN      
        1 ccgtgagtcc accacgccgc ggccagtcgc cttcttcgcg ccggatcgtc agggcatcgc
       61 gccgttgccg gttttcgagc aatacgacgc caagaatggg cagactttga gcgttgcggt
      121 aaatggccag gcccccgacg gatctgatgc tcatcctctg tttttcctac tccctgcgga
      181 cctcaaagac tacactgcgg cgacggtccc tttttatgag taccgcgagg aaggaagcgg
      241 gaggcgcgtc tattcggttg aagcacccag cgctgtcgcc cgctcgcact ggacgcccgt
      301 gctactcggt cgcgtctggc gaaacccagc gcgaatgcgg ctttggtgat gcgtggcgaa
      361 agtgggtcgc agccccgcgc gttacttttg gggattcagg aagaaatacg atgaaatcgt
      421 ccccgcccca acccgatgac acgcccacgc ctgactgcga tatgccggac aaacggatca
      481 cgcccggcga tcgtttgact ctgtcgcgcg agcggagctg gctcgaggaa atcgcccttc
      541 agctggagaa caatccggac gagtgctggc aagcgatcga gagcctggcg caagtccagc
      601 atgagttgcg aatgtcgatc atcgatgaac tgtcggttct cgggcccagg cccggcgccg
      661 ccaagttgct gcggctattg tcttcgacac gcgatcccgc gacgcgctcg gcggcgcaat
      721 ccgcgcttga gcgaatcgtc tggcacgccg atagtattcc caaactcatg gcgtcatcaa
      781 cattggcagt agtgacggcg agaactggcg tcgaaaaggc cctcggctac ggtcggccgt
      841 gtctctcgtt ggcgggtcgc aacggatccg ggccgcgttt ggcgcgctgc ctggtcactc
      901 cggtcgatgg acggggtcga gccacgatcg tggtttcggt caaccagatg catcagcgcc
      961 gcaccgcggc gttcctgtgc gacgtggagc gcggcatccg cgacgtcgtt ggagaagtcg
     1021 agcccgactc ggatcacgcc ggcggtttga tcgatgcatt cgaccaacag ccggcgggcg
     1081 attgtgcccg cgatgttccc gaactggcac ttcttctgct cgcggggagc ctgatgctta
     1141 ccgacgtgaa tgccactcac gcggtggggg agtggcttct tggcacgcta gggcccgatt
     1201 ttcggccggc ggaactcccc acggtgattt cggacttgaa cgcctcgtcg atcgaccacg
     1261 aggaaatgcc gcaacgtgcc ctgtccattc tcgaggcatg cccatcgtgg ctagatgtct
     1321 cgcccttgac cttcgagctc gctcgggaga tctggctccg cgaggggaga gacgccgccg
     1381 atcccacgcg cgatgcggga gcttaccgct accttttcga gcaccggctg agccgccgac
     1441 tggaattgta cgggcggatg ctcttgtgga tggcttggct ctggaaattc tcggggctgg
     1501 ccgacctatc cctttccgcg gcgacgatgg ccgctcaact ctcggacgaa caatacgctg
     1561 tccccagtca ccccttcacc gtcgagctga cgacacgaag tctcctggca gctcaggcca
     1621 gaatgcgcac ggcggatgat ccgcggaaag agcatcgagc atgagtctga aaaagtctcg
     1681 cgctttaccg acgtcgatcg gctcgtgaat cagcccggat ccaggatcgc ggcgctgtat
     1741 tagagcggct tgccccccag atccgggcgg cgatccgttg ctggacgacc attacttttt
     1801 ggtcggtttc gccgcagcag aggcctttgc ggcgggagca gccgatttcg catcgggctt
     1861 gctcgccttc ttcttctcat ccgccttttt cttcttggcg gtgtcacgtg cgtcgcccat
     1921 tgctcagtct ccttgtgaac gtgtcagaga tcccaaagtc gaaatgagct cgttatccct
     1981 tcaaacgaaa ggaacaacga gtcttaggat gacattatga ttcgcgttca tcaagagaga
     2041 ttgtgaagga atttgatgtt gggctgcttt ctcatgaact tgatcagagg ttggctcaca
     2101 tggcatgaaa acacaaacgc ctcgagggcg atcgttcgtc ctcgaggcgt tgcgtttaga
     2161 ggccggcatc tggtccagcc ggcttcaaga gttctcgatc agagcggtac gtagcaagac
     2221 cgcgctgggt cgcggcattc gcggcctccg gccatttcat cgtcgggccc gaagctcttc
     2281 atggtttccg aacaccagta ggacgagctg tcgtagttgt ccgctgtctc ctcgcgtgga
     2341 ccatccgtaa agacatacat acccgcgctg cggagatggc aacagggcga gcgctgcgtg
     2401 cccaagaccg gaaggtcaga cggtttggtg gagtccatgg acttgaggct attcaagcct
     2461 cctcccagta acggttgccg acagcggcga gcagggcccg cttgacgaca gtcttggtca
     2521 gcgggacctt gtagccgttc atggacagtg ggaccgcgcc ttccgccgcg gcctcgccgg
     2581 cagcggcggc tgtctcgatc gtcacacgct tgccggtgat cactttctcc gccgccgt
//
LOCUS       DATAQL010001125         4998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2711928, whole genome shotgun sequence.
ACCESSION   DATAQL010001125 DATAQL010000000
VERSION     DATAQL010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711928"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="VHS97_19430"
     CDS             complement(<1..2022)
                     /locus_tag="VHS97_19430"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX3450436.1"
                     /translation="MLATSSLTDARTRVLKHGETFAVFDRYGDATPGGLGEQGLFHES
                     TRFLSCSILELEGQRPFFLSSTVHEDNDRFVAHLTNPDLLAEGQLRAPLGTLHLAVEK
                     FLWQAACYQTVHISHFGHEAVDITLCLKFGADYADIFEVRGQHRPARGRDLAPELSGD
                     QVILAYHGLDDVVRRTVLRFAPPPLTLTHGEASWKLTLSPGAEHSLTVEIGCQRQPDP
                     QPLRSLTMARDEASVEHGRSQAWQCRLTSVNGQLNAWFNRAVSDLHMLVSHLSTGPYP
                     YAGLPWFNTPFGRDGLITALECLWVRPTLAKGVLQYLASTQATVVDREKDAEPGKILH
                     ETRNGEMADLKEMPYGQYYGSVDATPLYVLLAGAYFERTGDLESIQALWPNIVSALNW
                     IDVFGDLDGDALVEYQRKTPQGLLHQGWKDSDDAICHADGSPAQGPIALCEVQGYAYA
                     AWQAGARLAEALGHFENARTFQAKADAVRLRFEELFWCEEIGTYALALDGSKQPCRVK
                     SSNAPHVLFTGIADPSRAQRIAGTVLHPELFSGWGVRTLAVGEARYNPLSYHNGSVWP
                     HDNALIARGLARYGDTKSAARLLAGLFEAGQYFDLNRMPELFCGFTRHGGIGPIPYPV
                     ACSPQAWSAASVFLLLQASLGLEIDAIAGRVVFSRPALPLALPELRIFDL"
     gene            complement(2097..3140)
                     /locus_tag="VHS97_19435"
     CDS             complement(2097..3140)
                     /locus_tag="VHS97_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3450437.1"
                     /translation="MRIAQVAPLNESVPPLLYGGTERVVSYLTEELVRQGHEVTLYAS
                     GDSQTRARLVPGCPQALWRDPQCRETLPHHVRLMELVFHDRDRFDILHFHCDFLHFPI
                     LRRYPCPNVTTLHGLVHPPDLEPLLREYREMPLVSISDSQRRPLPWANWQATVYHGLP
                     ADLFSFHDKPNDYWAFLGRISPDKGVDKAIAIAREARKKLKIAAKIYPEDQGYFQQVI
                     APLLSEAGPQVEFVGEVGGAAKESFLGRAQGVLFPIGWPEPFGLVMIEAMACGTPVVA
                     FRRGSVPEVMTQGVTGYVVDDVAGAVDAVAKAAALDRRACRAAFEARYSAATMVRNYL
                     NVYDRLIAAHEPF"
     gene            3375..4805
                     /locus_tag="VHS97_19440"
     CDS             3375..4805
                     /locus_tag="VHS97_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450438.1"
                     /translation="MLDVEPIFVIPTNRLRDVGQTVEAYDEHFRRNGHAVTMIVCDDS
                     SVVNQQKYYGSLEKTRTHNPLYYVGPQEKEQFLFYLFDRLRDRKLNLLVRNLFRPSYG
                     GNRNYTLMYTLGHLMISSDDDMRPYALVEDSPESLGPDEISRGKLLKAAAKGYTRKSF
                     DILAAFLDVLGKPVGETPANYDRGELIVDSSMELETNASRGLARDNVLMLRQGAVADT
                     AMIKMAQTFRSGTNDIDALDFVEIFLNDESNISLDDLNELYVLVNFRPVITDMNWRMD
                     CGVAGYDNTYGLPPFFPTRLRFEDYIYRIWIQQDELASAHVDAAQNHVKNNYMRNPMA
                     SEVFNEEVANLLKRKIRNTVTRMDQLSIMFDYEGEVSLEDSEEILTRISDLHKRVVEG
                     QKSASSPERREVLRGFAANLDRAFYGFEPDFFQQNLIRIVDDVISQFKGSLEIWPTLV
                     EICYLHKNRAGLPMRAVENQRYSLAR"
BASE COUNT         1080 a         1463 c         1498 g          957 t
ORIGIN      
        1 gaggtcgaag attctgagct cgggtagcgc caacggcaga gcagggcggc tgaaaaccac
       61 tcgtccggcg attgcatcga tctcgagccc caaactcgcc tgcaggagga gaaagacgga
      121 agcggccgac caggcctgtg gcgaacaggc cacggggtag gggatcgggc caatcccgcc
      181 gtggcgcgtg aacccgcaga atagctcggg cattcggttg agatcgaagt attgtccggc
      241 ttcgaagagc ccggcgagca gtcgcgcagc gcttttggta tcgccgtacc gagccaagcc
      301 ccgggcgatc aaggcgttgt cgtgaggcca gaccgatccg ttgtgatagg acaacgggtt
      361 gtatctggct tccccgaccg ccaaggttcg cacgccccag ccggaaaaaa gctccgggtg
      421 caagaccgtt cccgcgatcc gctgcgcacg gctgggatca gcgatccccg tgaagaggac
      481 gtgcggggcg tttgaagact ttacccgaca cggctgtttt gaaccgtcaa gcgccagtgc
      541 atatgtgcca atttcctcac accagaagag ttcttcgaat ctcagacgca ctgcatctgc
      601 cttagcctgg aatgttcgcg cgttttcaaa atgacccaga gcctccgcca ggcgtgcgcc
      661 tgcctgccaa gcggcgtacg catagccctg gacctcgcag agcgcgatcg ggccctgagc
      721 gggtgaaccg tcggcgtggc agatggcatc gtcggagtct ttccagcctt gatgaagcag
      781 gccttgcggc gtctttcgct ggtattcgac aagggcatca ccatcgagat cgccaaatac
      841 gtcgatccag ttgagggcag acacgatgtt gggccagaga gcttgaatcg attccaggtc
      901 gccggtgcgc tcgaaatacg cacccgccag caagacgtac aacggcgtgg cgtcgacgct
      961 gccatagtac tggccatagg gcatttcctt gagatcggcc atctcaccgt tacgggtttc
     1021 gtggaggatc ttgccgggct cggcatcttt ttcccgatcg acgacggtcg cctgggtcga
     1081 cgccaagtat tggaggacac ccttggcgag ggtcggccgg acccagaggc actcgagcgc
     1141 cgtgatgaga ccgtcgcggc cgaacggcgt gttgaaccag ggcaggcccg cgtacgggta
     1201 tggccccgtt gacaggtggc tcaccagcat atgcaggtcg gagaccgccc ggttaaacca
     1261 ggcattgagc tgtccattga ccgacgtgag ccggcactgc caagcctggg accggccgtg
     1321 ctcgacggag gcttcatcgc gcgccatagt cagggatcgc aacggttgcg gatcgggttg
     1381 ccgctggcag ccaatctcga ccgtgagcga atgctcggcg ccgggtgaga gagtgagttt
     1441 ccatgaggcc tcgccgtgcg taagggtcaa gggaggcggg gcgaagcgca agacagtgcg
     1501 ccggacaacg tcgtcgagtc catggtaagc aaggataact tgatcgccgg ataattcggg
     1561 agccaggtcg cgcccgcggg ctggccggtg ctggccgcgg acctcgaaaa tgtccgcata
     1621 gtcggcccca aacttgagac aaagtgtgat gtcgaccgcc tcatgtccga agtggctgat
     1681 gtgtaccgtc tggtagcagg ctgcctgcca gaggaacttt tcgaccgcca gatgcagcgt
     1741 tccgagcggg gcacgcaact gaccctcggc caacagatcc gggtttgtca gatgcgccac
     1801 aaatcggtcg ttatcttcat gaacggtcga gctcaaaaag aacggccgct gcccttcgag
     1861 ctcaaggatc gagcacgaca agaaccgcgt gctctcatga aagagccctt gttcgcccag
     1921 gccgccgggc gtcgcgtcgc cgtagcgatc gaacaccgcg aaggtctcgc cgtgcttgag
     1981 cacgcgggtc cgcgcatcgg tcaagctgga tgttgccagc aagtgatatg gtccgatatc
     2041 ggaggaattc acttgtgttt gggttttccc ttaagtggaa tgaagccatc cagctttcag
     2101 aatggctcgt gagcagcgat taatcgatcg taaacattca ggtagttccg aaccatcgtg
     2161 gccgcgctat aacgcgcttc aaatgcggcc cggcaagccc ggcgatcgag agcggcagcc
     2221 ttagccacgg catcgaccgc gccggcgaca tcgtcgacca catagccagt caccccttgg
     2281 gtcatgacct ctgggaccga accgcgccgg aatgcgacga cgggcgttcc acacgccatt
     2341 gcctcgatca tgaccaggcc gaatggctca ggccagccga ttgggaacag cactccctgc
     2401 gctcggccga ggaacgactc tttggccgcg ccgccgacct caccgacgaa ctcgacctgc
     2461 ggcccagcct ccgacaagag cggggcgatg acctgctgga aataaccctg gtcctcggga
     2521 tagatcttgg cggcgatttt gagctttttc cttgcttctc gtgcgatggc gatcgctttg
     2581 tcgacacctt tatcgggtga aattctcccg agaaaggccc agtaatcatt tggtttgtcg
     2641 tgaaacgaga aaagatccgc cggtagaccg tggtagacgg tcgcctgcca gttggcccag
     2701 ggcagcggcc gccgctggga gtccgagatc gagacaagtg gcatttcgcg atattcgcgc
     2761 aagagcggct cgaggtccgg cggatgtacc aggccgtgca gggtcgtcac gttggggcaa
     2821 gggtagcgcc gcaagattgg aaagtgaaga aaatcacagt ggaaatggag aatatcgaag
     2881 cggtctcggt cgtggaaaac cagttccatg agccggacgt gatgcgggag cgtctcgcga
     2941 cattgtggat cgcgccaaag cgcttgtggg caacctggca cgagccgagc cctggtttgc
     3001 gaatcgccgc tcgcatagag cgtcacctcg tgcccctggc ggaccagctc ttcggtcaag
     3061 taagaaacaa cccgctccgt accaccgtaa aggagcggcg gaacgctttc attgagcgga
     3121 gcaacttgcg cgatgcgcat gaaaacaact cgggaggccg acaatcgatc gcaacacgcg
     3181 cggacgtcct tgcattctac gtggttaagt gcgatctcgc tattcgcctt ccacgcccgc
     3241 ggaacgagtg gttattctgt atcgagtcaa ccggtgagtg ccgaagtgcc cccagttgcc
     3301 gcgatcgtcc cctggccggg ttgccgagca aggtttgact ttcgcaatca cattggaata
     3361 tgaaacaagc accgatgttg gatgtcgagc cgatctttgt gataccgacc aatcgcctgc
     3421 gcgacgtcgg ccaaaccgtg gaagcctacg atgaacactt ccgccgcaac ggacatgccg
     3481 tcaccatgat cgtctgcgac gactcgagtg tcgtcaatca gcaaaaatac tacggatcgc
     3541 tggaaaaaac ccgcacgcac aatcctctct actacgtcgg tcctcaagag aaagagcagt
     3601 ttctcttcta tcttttcgac cgtctgcgcg accgcaagct caacctgctg gtgcgaaacc
     3661 tgttcaggcc cagttacggc ggcaatcgca attacacctt gatgtacacg cttggtcatt
     3721 tgatgatcag ctccgacgac gacatgcgcc cgtacgcctt ggtggaagat agcccggagt
     3781 cgctcggccc agacgagatc agccggggca agctcttaaa ggcagccgcc aagggataca
     3841 cgcgcaagtc gttcgatatt ctggccgcct tcctggacgt cctgggcaaa ccggtcggcg
     3901 aaacgccggc caactacgat cgaggcgagc tgatcgtcga ttcgtcgatg gaactggaaa
     3961 ccaacgcctc gcgcggactg gcgcgcgaca acgttttgat gctacgccag ggtgcggtgg
     4021 ccgacaccgc catgatcaag atggcccaga cctttcgttc cggtaccaac gacatcgacg
     4081 cactcgattt cgtcgagatc tttttgaatg acgagtccaa catcagcctc gatgatttga
     4141 acgaactgta cgtgctcgtc aatttccgac ccgtcattac cgacatgaac tggcgcatgg
     4201 actgcggcgt cgcgggctat gataacacct acggtctccc cccatttttt cccacccggc
     4261 tccgtttcga ggattacatt taccgcatct ggattcagca ggacgagctg gcctcggcgc
     4321 acgtcgacgc ggcgcaaaac cacgtcaaga acaactacat gcgcaacccg atggcatcag
     4381 aagttttcaa cgaggaagtg gccaacctgc tcaaacgcaa gatcagaaac accgtcacgc
     4441 gcatggatca attaagcatc atgttcgatt atgaagggga agtaagcctt gaggacagcg
     4501 aagagatctt gacgcgcatc agcgacctgc acaaacgagt ggtggaaggc cagaagtcgg
     4561 cctccagccc ggagcgtcgg gaggttctcc gtgggttcgc cgcgaatctg gaccgagcgt
     4621 tctacggctt cgagcccgac tttttccagc aaaacctgat ccggatcgtg gacgatgtga
     4681 tcagccagtt caagggttcg ctcgagatct ggccgacctt ggtcgagatc tgctatttgc
     4741 acaagaaccg cgccggtctg cccatgcggg ccgtcgagaa tcagcgttat tctttggcac
     4801 gctaggtggg tccgagcgaa gccgaaatat gaaaacaggg tcggtccttg caggatgaac
     4861 tgccaagccg tagttttcat cggattttct tgccaagacc aatgccgcca aaacctcgtc
     4921 gtgagacgtg attcgagtga ccatagccac cgaacaagct gtcgtccccg cacgaccgac
     4981 gcgcgggcgg tttgaaat
//
LOCUS       DATAQL010001126         8955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3627472, whole genome shotgun sequence.
ACCESSION   DATAQL010001126 DATAQL010000000
VERSION     DATAQL010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3627472"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..817
                     /locus_tag="VHS97_19445"
     CDS             212..817
                     /locus_tag="VHS97_19445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3450439.1"
                     /translation="MTLSPPRYGGASARKVWLTFDDGPHPQYTDRVLDVLQSYGISAT
                     FFVLGASVDRWGTTVLERMGTEGHSIGNHAYSHTDLTSLTENEVRDEIKRTEAMIGRL
                     PNAEKVFRPPYGASSPTVDRVARELDYRKVLWNVDTRDWDLAFQPNHWVESALCPVLR
                     LKRAIVIAHDVHKTTADHLPDLIERIGHACFERCTARKRAS"
     gene            1236..3047
                     /locus_tag="VHS97_19450"
     CDS             1236..3047
                     /locus_tag="VHS97_19450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3450440.1"
                     /translation="MNRHPQNCEYRCRVREQSGLEVADCLLLETITGASGAGLCTVQK
                     TACESCCATFPPTIDDLNPVLASFLYKVASTVIELGGVSGCDVSNAANLQMRAELKLS
                     ARLAPQAGTVACDVVLCCDKSSTEADRAIQSVLSQKGAHAFLHLVDDGGGGAEVVRRY
                     SGFNHVFTYTNPVRRGALRTLHELVPRFRSSYVAVQDPGTVSTPERLRTAVSMLEAYG
                     AEILAAPVNTPSGLIFPRRPRTEYHRYLPLQSLVCRRASLVDMGGIADRPTDADAELI
                     YRAFREERRIVFAEEPAVDSFLHLDDGELGAPPRYEVRQGALRHHAIGFEQESVACDV
                     VLPFHGHLEFLRESLPSVLEQEGAETIVHLIDDATPGGADEPLRYWGSHPRVRTYRNS
                     RNLGQFVSFNNVFPYLETNLLAIQDADDISLPQRVRLAGNHLRIADADFFGGRFETFE
                     HHEDQQTSESTRRPELAPVEKPPYWASEYPRRYERIHFLQNTTAVMRRGAFESLRGFS
                     DYGEVERNKCGLDTEFFVRAHHAGCRFALTQDVVVRYRWHRKSATRNSQTGWGTEPRF
                     WSAAENERRFKLFHRGPFDVRALGSLHQSSGLTRRIT"
     gene            3071..5158
                     /locus_tag="VHS97_19455"
     CDS             3071..5158
                     /locus_tag="VHS97_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450441.1"
                     /translation="MARGRHRSTTPEPEMVVFPVIVAFQTGDWLPIAVESYLAQFPAD
                     RILVVDNNPGPGETGWSAACRRERRWLYSHPRIDVIHQHANCAGIPARRTHGDGMDLA
                     LAWCRSRGADVMLHIEPDCLVTGRRWRENLVGAIEQGAWMAGAHRKAWGPIHPTPSAW
                     LVREVRTSFAEQSLPRSVAHPRFNDLVDLEVLRAAAELDGCWEWAQRRWDTAHMAWFQ
                     AAVHDRTSLVDAPGFQHFWVGTSSNCLPLEQLVIKFPELASWFARPRAAGGPRRVEDC
                     SFRHDPRRDTDIELACCKLLKQLSGVMEPRLCDVRRDACEACCKSADPSLLTINSTVA
                     SLLFRLADRIVGRGGVVGCDAQSAMHLRAFAEDYLELRFPGEGSAPAPRRTASPCLHL
                     GGQVGSRVLVTASGFDRLSVYECKHPDHFETTTPECGGCRDWTDKPLIESVPIEQLVP
                     RPVHRFGRRIQTWAVGVTTAPRPQPTVHACLDSLSRVGWKEPRLFVDSGVTIADRFSE
                     LPLTFRETKLGPWPNYYLALVELLMREPEADAFMLVQDDVIFDDRHDVRTYLEEILWP
                     ADPIAAVSLYCSKAYTRPVAGWHELNSQWIWGALAFVFPRESAKRFVTDPLVFEHRSN
                     PTEGLVNIDILIGRWAHERQLPIYFPSPSLVQHIGNESSIWSDPDSRAIGDRRADRFA
                     GDIDRAPLEYPAT"
     gene            complement(5231..7357)
                     /locus_tag="VHS97_19460"
     CDS             complement(5231..7357)
                     /locus_tag="VHS97_19460"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEX3450442.1"
                     /translation="MPCYGIDLTCHQDEVRGMSPYRSIICWATAAFAVAVALVCLIAG
                     RGPIPSRLPLSSDGDSGASPFMKLAAFNQGGTPSLRGGVDWINSGPITLSELHGKIVV
                     LDFWTFCCINCHHVLPDLARLEEKYKNELVVIGVHTAKFPAERETENIRRKVREYRIK
                     HPVINDANQTIWNRFGVNSWPTLVLIDAKGNPVEAFSGEGNYDVLDRTIGQLVEGHRA
                     RGELNLTPFKFNPEMERPSDGPLLFPGKVLADSSGKRIFIADTGHNRIIQTNFDGAEP
                     VKIGSGVEGFDDGESDKATFNRPQGMCLEGETLYVADTENHAIRAIDLKDHSVTTLAG
                     TGSQARGIPRPGAFGPAKSTPLCSPWDVIQLAGDKAIYIAMAGPHQIWKLDRGTETVS
                     VFAGSGVENIRDGTLASANFAQPSGLATDGENLFVADSEVSGVRVITGIRGREPGVRT
                     IVGQGLFEFGDKDGRGAAVRLQHCLGLAYTGGHLYIADSYNNKIKICEPRNRSVRSLV
                     GSHKSGDSDNPPHFYEPGGLSATEDRLFVADTNNHKIRVVDLKTHAVKTLALEGLTAP
                     KLAPRPPSFPNAKIIDIASTKVSAGSSIAVAVSIALPKGYKLNPEVPLTYLVETPEKS
                     GVLGSENLPEGHKIKPPVAKFMIDVPLAKPATVGEKVDLRLSLQTFVCSETSNLCAIK
                     SYIWKVPIVFSDDVSTAPLSLNTDTK"
     gene            7612..8604
                     /locus_tag="VHS97_19465"
     CDS             7612..8604
                     /locus_tag="VHS97_19465"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX3450443.1"
                     /translation="MDKSLICYDDASKKNDPYGELKVFAGSSGKGLGRAICDHLDMEL
                     ARSETHVFSEGNVFVRVLENVRGRDVFVVQGTDYPVNDNFVELLFWIDAFKRASATQV
                     TAVIPYFSYAKGDKKDEPRVSIRARVCADCIEAAGADRVLTMDLHSPQIQGFFKIPVD
                     HLYAMPVFVEYFRAREIPDLVVAAPDVGFGKQAYKFGEMMHAPVVFGNKTRRGHDEKA
                     EMLDIVGQVSGKNVLIVDDFTISGGTLIEMAITCKKRGAKDIYACVSHGVFSRGSAAK
                     IEQSPIKELILTDTIEYRFEPLPPCCKIISVSKLFADAVISIHRRESVSRLFNY"
     gene            8753..>8955
                     /locus_tag="VHS97_19470"
     CDS             8753..>8955
                     /locus_tag="VHS97_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450444.1"
                     /translation="MNRVVAPIGLQLPRVVFGKVQKQVTPEGVAGVDTRRVTPPDLRL
                     PRRGLAGLSPARPQPPSPSHQLLS"
BASE COUNT         1733 a         2642 c         2726 g         1854 t
ORIGIN      
        1 agacaccgaa ccggtgagca acacgatcga ccgcgtgctg cacggcggct catatgataa
       61 ggtcatcgat cacgtacaat cggatcgctc cgaacatgcg attccgcaag tgaagttcaa
      121 ctcgatcggt tttcgcgtga cgaggactca cccatcgggg cgatgacttt cccgagattt
      181 tccatcgcgt tcgaggacga cggagatcgc cgtgacgctt tcgccacctc gttacggcgg
      241 cgcctctgcc aggaaggtgt ggctgacttt tgatgatggg ccacatcccc agtacaccga
      301 cagggttctc gatgtgctcc agtcgtacgg gatcagtgcc accttttttg ttttgggcgc
      361 gagtgtggat cggtggggaa cgaccgtcct ggaacggatg gggaccgagg gccattccat
      421 aggtaaccac gcatattcgc acacggatct cacatcgctc accgaaaacg aggtccgcga
      481 cgagatcaag agaacagaag cgatgattgg gcggttgcca aacgccgaga aggtgtttcg
      541 tcctccctac ggagcgtcga gccccacggt cgaccgcgtt gcgagggagc tcgactatcg
      601 caaggtcctc tggaatgtcg acacccgtga ctgggacctg gcatttcaac cgaaccactg
      661 ggtcgagagc gctctttgcc cagtcctccg gctgaagaga gcgatcgtca ttgcccatga
      721 cgttcataag acgaccgccg accatctccc cgacctgatt gaacggatcg gacacgcatg
      781 cttcgaacgg tgcacggcgc ggaaacgtgc gagttgacat agaagcgaac agccctcaga
      841 ctgtaatgcc gtttgaacac ggcctttcgc ggtcgaatat gaagtactcg atcgggacct
      901 ctacacatcc agagcaccac acacaggggt tcacgcatgc cagccaaaca cgagattccc
      961 aacgcggcgg tttccacggc acgcccagcg aatgcaatcc tagactctcg agatctcgga
     1021 gtcggcgctg tcctctggga atcggtggcg ggcgaatgga agctcatcaa atgcggatgc
     1081 tcagagggat atgaggtcac tgatgcgccg acggagcctg gaatgtacga agggcaaatc
     1141 gtgaagacgc tgtgcgagcc agttgcgcgc taaggtgccg gcgtgcttgt cgacgcgact
     1201 tcgaggcgcg acgggacgtc catcggcggg ccacgatgaa tcggcatccc caaaattgtg
     1261 aatatcgctg ccgggtccgg gagcaatccg gactggaggt cgctgactgt ctcctgttgg
     1321 agacgatcac cggagcgagc ggcgctggac tttgcaccgt gcaaaagacc gcctgcgaat
     1381 cgtgttgcgc aaccttcccg ccgacgatcg acgatctcaa tcccgtgctg gcgtcgtttc
     1441 tctacaaggt cgccagcacc gtgatcgagc tgggcggggt ttctggctgc gatgtcagta
     1501 acgcagcaaa cttgcagatg cgcgcggagc taaagctgtc tgcacgcctc gcgcctcagg
     1561 caggcacggt cgcgtgcgat gtggtcctat gctgtgacaa gagttccacg gaggccgatc
     1621 gggcaataca gagcgtgctc agtcagaaag gtgcccacgc ctttcttcac ctggtcgatg
     1681 atggtggcgg cggcgcggag gtcgtgcgac gttactccgg attcaaccat gtcttcacct
     1741 atacaaaccc cgtccgtcga ggcgcactgc gcacgctgca cgaactcgta ccgcgttttc
     1801 ggtcttcgta tgtcgccgtt caggacccgg ggactgtcag cactcccgag cggctccgaa
     1861 ccgccgtctc gatgctcgag gcctacggtg ctgaaattct cgcggccccg gtgaacactc
     1921 cttcgggact tatctttccc cggcgcccac ggacggaata tcatcgctac ttgccgttgc
     1981 aatcgctcgt ttgccgcagg gccagcttgg tggacatggg aggaatcgcg gatcgaccta
     2041 cagacgccga cgcggaattg atttaccggg cgttccggga agagcgcaga attgtcttcg
     2101 ccgaggaacc ggcggtcgat tcgttcttac acttggatga cggcgagctc ggagcaccgc
     2161 ctcgctacga ggttcgacaa ggggctctcc gtcatcatgc catcgggttc gagcaggaat
     2221 cggtcgcgtg cgacgtcgtg ctgccgttcc acggccacct cgagttcttg cgggaatcgc
     2281 tcccgagtgt gctcgagcaa gagggagcag aaacgatcgt acacttgatc gatgatgcca
     2341 cgcctggcgg agcggacgag ccgcttcgtt actggggcag ccatccgcgc gtacggacct
     2401 accgcaacag ccgcaatctc ggtcaattcg tttcgttcaa taatgtcttc ccgtatctgg
     2461 aaacgaatct cctcgcaatc caggacgccg atgacatcag tctgccccag cgcgtgcgcc
     2521 tggcgggcaa ccaccttcga atagcggacg ccgacttttt cggaggacgt ttcgagacct
     2581 tcgagcacca tgaagatcag cagacgagtg aatcaactcg caggcccgag ctagctccgg
     2641 tggaaaagcc accctactgg gccagcgaat acccgcgcag gtatgagcga atccactttt
     2701 tacagaatac gaccgctgtg atgcggcgag gggccttcga gtcgctgagg ggctttagcg
     2761 attacggcga ggtcgaacgc aacaagtgtg gactggacac cgagttcttc gtgagggcgc
     2821 accacgccgg ctgccggttc gccctcacgc aggatgtcgt cgtccgctac cgatggcaca
     2881 ggaagtccgc cacgcggaac tcgcagacag ggtgggggac tgagccacgc ttttggtctg
     2941 cggccgaaaa cgagcgccgg ttcaagctct ttcaccgtgg accattcgac gtgcgcgctt
     3001 taggaagcct gcatcagtcg tccggactga cccgcagaat cacatgatga tgccgctcgc
     3061 tgtccgtagg atggcacgcg ggcgtcatcg ctccaccacc ccagagcccg agatggtcgt
     3121 tttccccgtg attgtggcgt ttcagacggg cgactggctg ccgatcgccg tcgagtcgta
     3181 tctggcgcag tttcccgcgg accgcattct ggttgttgac aacaatcctg ggcccgggga
     3241 gaccggatgg tcggcggctt gccggcgcga gaggcgctgg ctgtactcgc atccgagaat
     3301 tgacgtcatt caccagcacg ccaactgtgc cgggatcccg gcacggcgaa cgcacggcga
     3361 tggcatggac ctggcgttgg cctggtgccg cagccgcggc gccgacgtca tgctccacat
     3421 cgaaccagac tgcctcgtta ccggacgtcg atggcgtgaa aacctcgtgg gcgccatcga
     3481 gcagggtgcc tggatggcgg gggcgcatcg aaaggcttgg gggccaatcc atccgacgcc
     3541 gtcggcctgg ctggtgaggg aagtcaggac gagtttcgcg gagcagtccc tgccccgaag
     3601 cgtcgctcat ccgcgcttca acgatcttgt cgatctcgaa gtgctccggg cagccgccga
     3661 gttggacggt tgctgggaat gggctcaacg acggtgggac acagcgcaca tggcctggtt
     3721 tcaggccgcc gtccacgacc ggacctcact ggttgacgcg cccgggtttc agcatttctg
     3781 ggtcgggacg tcgtccaact gtctcccgct ggagcaattg gtcatcaagt ttcccgagct
     3841 ggccagttgg ttcgcccgcc cgagggcggc aggcggcccg cgccgcgtgg aagattgttc
     3901 cttccgacac gacccgcgac gagatacaga tatcgaactc gcgtgctgca agctgctaaa
     3961 gcaattaagc ggcgtcatgg agccccgatt gtgtgacgtg cgtcgcgacg catgtgaggc
     4021 ttgctgcaag tcggccgatc cgtcgctctt gaccatcaac tccaccgtcg catcgctcct
     4081 ctttcgtctc gccgaccgga tcgtcggtcg aggtggcgtc gtcggctgtg atgcccaaag
     4141 cgccatgcac ctgcgggcgt ttgccgaaga ttatctcgaa ctccgttttc cgggcgaagg
     4201 atccgcgccg gcgccgcgac ggaccgcgag tccgtgtctt cacctgggcg gtcaggtcgg
     4261 ttcacgtgtg ctggtgactg catcggggtt tgaccggctc tcggtttacg agtgtaagca
     4321 ccccgatcat tttgagacga cgacccccga atgcgggggg tgtcgcgatt ggacggacaa
     4381 gcctcttatt gaatcagttc cgatcgaaca gctggtgccg cgccccgtgc accgtttcgg
     4441 ccgacggatc cagacatggg ctgtcggtgt gacgaccgct cctcggccgc aaccgaccgt
     4501 ccacgcgtgc ctcgatagcc tgtcccgggt cggctggaaa gagccccgtt tgtttgtaga
     4561 ctcaggcgtg acgatcgccg accgctttag cgagctgcct ctcacatttc gtgagacgaa
     4621 actaggtccc tggccgaatt attatcttgc cctggtcgag ctcttgatgc gggagcccga
     4681 agccgacgcc ttcatgctcg tgcaagacga tgtcatcttt gacgaccgcc acgatgtacg
     4741 gacttatctt gaagaaatcc tttggccggc cgatccgatc gccgctgtgt cactctattg
     4801 ctcgaaagct tacacacgtc cggtggccgg ttggcacgag ctcaacagcc aatggatctg
     4861 gggagcgctc gcgtttgtct ttccacgtga gtctgccaag cggttcgtga ccgacccgct
     4921 cgtgttcgaa cacaggagca acccgaccga agggctggtc aatatcgaca ttctgatcgg
     4981 acgctgggct cacgagcgtc aactgccgat ttactttcct tcgccgagcc tggtgcagca
     5041 catcggcaat gaaagctcaa tctggtccga ccccgattcc cgggctatag gtgaccggcg
     5101 cgccgatcga ttcgccggag acatcgatcg ggccccgctc gagtatccgg cgacgtgaat
     5161 atcagatcag ccgaaggttt acgtgtacga catgtgatcg tcgcaatttc gaacaacctg
     5221 tggtctcgag ttacttcgtg tcggtattga gcgagagcgg agcggtgctg acgtcgtcgc
     5281 tgaatacgat tggcactttc cagatgtaac tcttgatggc gcagaggttc gatgtctcgc
     5341 tgcagacaaa tgtctggagc gacagacgca gatcgacctt ctcgccgaca gtggcgggct
     5401 tggccagcgg cacgtcaatc atgaacttcg cgaccggtgg cttaattttg tgaccttccg
     5461 gcaagttttc agaaccgagc acgccgcttt tctcgggtgt ttctaccaga tatgtcagcg
     5521 ggacctctgg gttgagcttg taccccttgg gtaaagcgat cgagacagcc accgcgatcg
     5581 acgaaccggc ggacaccttg gtggaggcaa tgtcgatgat cttggcgttc gggaagctgg
     5641 ggggccgagg cgccaacttc ggggccgtga gaccctctag cgccagcgtc ttgaccgcat
     5701 gggtcttgag atcgacgacg cggatcttgt ggttgttggt gtcagcgacg aacaggcggt
     5761 cttcggtcgc actcaagccg cccggttcgt agaaatgtgg aggattatcg ctgtcgcccg
     5821 acttgtgcga gccaaccaac gacctgaccg atcggttcct cggctcgcag atcttgatct
     5881 tgttattgta actatcggcg atgtagaggt gcccgccggt gtacgcgagt cccaggcagt
     5941 gctggagccg gacggccgct ccccggccgt ctttatcgcc aaattcgaaa aggccctggc
     6001 cgacgatggt tcgcacgcct ggttcccggc cccggatgcc cgtgatcacg cgcactccgg
     6061 agacctccga gtcggcgacg aagaggtttt cgccatcagt ggccaggccg ctcggttgcg
     6121 cgaagttagc cgaggccaac gtgccatcgc ggatattctc cactccggat ccggcgaaga
     6181 cactgaccgt ttcagtgccg cggtcgagct tccagatctg atgtggaccg gccatcgcga
     6241 tgtagattgc cttgtcaccg gcaagctgga tcacgtccca ggggctgcaa agcggagtcg
     6301 acttggccgg accgaaagca cccgggcggg gaatgccgcg ggcctggctg ccagtgcccg
     6361 cgagcgtggt caccgaatgg tctttgagat cgatcgcgcg gatcgcgtgg ttttcggtgt
     6421 cggccacata gagagtctcg ccttccaagc acatcccctg gggccggttg aaagtcgcct
     6481 tgtcagattc gccgtcgtcg aatccctcta caccgctgcc aattttgaca ggctcggctc
     6541 cgtcgaagtt ggtctgaatg attcggttat ggccggtgtc ggcgatgaag attcgcttgc
     6601 cgctcgaatc agccagcact ttgcccggaa acaagagcgg gccgtcggag ggacgctcca
     6661 tttcgggatt gaacttgaac ggagtcaaat tgagctcgcc tcgggcccta tggccctcaa
     6721 cgagctggcc gatcgtccgg tcgaggacat cgtaattgcc ctcgccgctg aaagcctcga
     6781 cgggatttcc cttggcgtcg atcaaaacca aggtcggcca gctattcacg ccaaaacgat
     6841 tccagatcgt ctggttcgcg tcattgatca ccgggtgctt gattcgatac tcgcgtacct
     6901 tgcgcctgat attctccgtt tcgcgctcgg cgggaaactt ggccgtgtgc acgccgatga
     6961 cgaccaactc gttcttatat ttttcttcga gtctcgccag gtccggaaga acgtggtggc
     7021 agttgatgca gcaaaaagtc cagaaatcga gcacgacgat tttgccgtgc agctcggaca
     7081 gcgtaattgg gccggagttg atccaatcga caccacctct caggctggga gtgccccctt
     7141 ggttgaacgc ggccagcttc atgaacggac ttgctcccga gtcgccatca gagctcaatg
     7201 gtaaccggct cggaatcgga ccgcggccgg caataaggca gacaagggca acggccacgg
     7261 caaacgctgc tgttgcccag cagataatcg agcgataagg actcattcca cggacctcat
     7321 cttggtgaca ggtcagatcg attccatagc acggcatcgc cgtgcgtttc cgcgtaaata
     7381 ttgtaatcga ccgctgggga tttggcttgt ctttttgcaa cgcggcggtt ttgtcgtgtt
     7441 ctattaggtc gagcgctgct ggcggtttcc ggctgaaaac gttaagatgc catgtagaag
     7501 ttcaggatgg gagatcgcgt gcttggagca acgcgtgggg attcactagt gtgatccgct
     7561 gaggttggcg gggttttcag cagccggtca cctggagcga ccaccgtggg catggacaaa
     7621 tctctgattt gctatgacga tgcatccaag aaaaatgatc cctacggtga gctcaaggtc
     7681 tttgcgggca gttcgggcaa gggattgggt cgagcaattt gcgatcacct cgacatggag
     7741 ctcgcgcgat cggagaccca cgtcttcagc gagggcaatg tctttgtccg tgtcctcgaa
     7801 aatgtgcgcg gccgcgacgt cttcgtcgtg caggggaccg actatcccgt caatgacaat
     7861 ttcgtcgagc ttttgttttg gatcgacgct ttcaagcgtg ccagcgcgac gcaagtcacg
     7921 gccgtgatcc cttatttttc gtacgccaag ggggacaaga aagacgagcc gcgagtttcg
     7981 atccgcgctc gcgtctgcgc cgattgcatc gaggcggcgg gggccgatcg cgtcttgacc
     8041 atggatcttc acagcccaca gatccagggg tttttcaaaa tcccggtcga ccatctctac
     8101 gcgatgcccg tttttgtcga atactttcgg gcgcgggaga tccccgacct ggttgtcgcc
     8161 gcccccgacg tgggcttcgg caagcaagcc tacaagtttg gcgaaatgat gcacgccccg
     8221 gttgtgttcg gcaacaagac gcggcgcggg catgacgaga aggcggagat gctcgatatc
     8281 gtcggtcagg tcagcggcaa gaatgtcttg attgttgacg atttcaccat ttcgggtgga
     8341 acactgatcg aaatggccat cacctgcaag aagcgcggcg ccaaggacat ttacgcctgt
     8401 gtatcgcacg gcgttttttc ccgcggttcg gcggccaaga tcgagcagag cccaatcaaa
     8461 gagctaatcc tcaccgatac gatcgaatac cgcttcgagc cgcttccgcc ttgctgcaag
     8521 atcatcagcg tctcaaagct tttcgccgac gcagtcattt cgatccatcg ccgcgagagc
     8581 gtgagccggt tattcaacta ctgaattggc cgggtcgtca accagacgga ctggcagcct
     8641 ttcatctcat ggcaacgcag cctcgaaggt ctctggcatc aggctgtagc tcgcgaccgc
     8701 gccgcgaatc gacaaggcgg cggcctagat cagtccgggt ccggggcgga cgatgaatcg
     8761 agtggtggct ccaatcggtt tgcaactacc acgcgttgtg ttcggcaagg ttcaaaagca
     8821 ggttacacct gagggggtgg ctggggtcga cacccgaagg gtgacgcccc cggatctgag
     8881 gcttccgcgc cgggggctcg ctggcttatc gccagctcga ccccagccac ccagccccag
     8941 ccaccagctc ttgtc
//
LOCUS       DATAQL010001127         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3322335, whole genome shotgun sequence.
ACCESSION   DATAQL010001127 DATAQL010000000
VERSION     DATAQL010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3322335"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..863)
                     /gene="soxR"
                     /locus_tag="VHS97_19475"
     CDS             complement(414..863)
                     /gene="soxR"
                     /locus_tag="VHS97_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEX3450445.1"
                     /translation="MADLLTITEISRRSGVASSALRFYEERGLIRSQRAGSGHRRYSR
                     AVLRRIAFVVFAQRIGMTLEEIGAELAKLPLDRVPEGSDWAKLSSAWVGRIDERMDEL
                     RRLRAGLTACIGCGCLSLDRCAFANPRDRARGQGPGPRYWVTDRKSP"
     gene            981..1721
                     /locus_tag="VHS97_19480"
     CDS             981..1721
                     /locus_tag="VHS97_19480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3450446.1"
                     /translation="MELCSNSGSNDRFHGLRVAVSGGTSGLGLALVRELFERGARVAF
                     VARTRRRVEQVASELAGVHGIVGDVSLKGDIHPIVLQILGALGGLDILINNASALGPA
                     PLALLGDTDCESLERALATNLVGPFRLTKALLGALFASAREGSGALVVNVSSNAAVSA
                     YPYWGAYGASKAALRHLSAIWDAELAPEGIQFVSLDPGDMDTPLHAIAVPDADRAALK
                     QPQVAAREMLDAIAAALPKYHSIPGAIL"
     gene            1718..2776
                     /locus_tag="VHS97_19485"
     CDS             1718..2776
                     /locus_tag="VHS97_19485"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX3450447.1"
                     /translation="MIAADSPVQRGPWAKLLVVRASGEMIHARRAGFVDFLRPGDVVI
                     ANDAATLPASLKGRHAPSGLPIEIRLAGRRSLGTDDVLQFSAVAFGSGDYQTRTEDRA
                     PPPRMVPGDRLDFGALSATVERLLGHPRLLSLRFDGAPDAVWSGLARHGRPIQYAHVR
                     MPLALWDVWTPIAGLPAAFESPSAGFALDWRSVAAMRARGIEFSTVTHAAGISSTGDD
                     ELDRRLPFDEPYRIPPSAAAAIARARSRGGRVVAIGTTVVRALEHAATVDHIVTAGAA
                     LATQRIGGPTILQVVDAVLSGIHEPGTSHYEMLRAFSSDETLARANDELVARNYFTHE
                     FGDSVLIERAQNDEFVSD"
BASE COUNT          548 a          964 c          943 g          574 t
ORIGIN      
        1 cgaaatacat gcacgcagtt ttcagggaat gacaagcgct ccgcgtcgga atgccgtctt
       61 cgacgctccg ggtcgtctcc aaccgctgga tcaccagtcg gctgtgacgg caaccaacaa
      121 ctcgatcggc gcataattcc cctctcgttc ccacgcagag agcgtgggaa cgagtgcgag
      181 cagaccaccc gtcaatcgaa ggttgacggg acactagggg ccttttgtag ggccagccat
      241 tgccgcccga aacctggccg gcgacggccg gccccgcggt gggacttcgc aacaggctct
      301 acggccaagc ggaagccgct ctagctgagg ttgtcccgat cttagttgaa aagtggcgag
      361 gacggttctt cccgtccggt cgctagactg gacgtggcgc cgcacatcgc agcttatggc
      421 gacttgcgat cggtcaccca ataccgcggc cctggtccct gaccacgggc acggtcgcgg
      481 gggttcgcaa acgcgcaccg atccaaagag aggcagccgc agccgatgca cgccgtcaga
      541 cctgcccgca gccggcggag ctcgtccatt cgttcatcga tccggcccac ccatgcgctt
      601 gaaagcttgg cccagtctga accctcgggc actcggtcca gcgggagctt ggccagttcg
      661 gcaccgattt cttccagtgt catcccgatc cgctgggcaa agacaacaaa cgcgattcgc
      721 cgaagtacgg cccgcgagta acggcggtgg cccgagcctg ccctctggga tcggatcagg
      781 ccccgctcct cgtagaatcg cagggcagac gacgccacac cgctccgccg cgatatctcg
      841 gtgatggtca gtagatcggc catgaccaac gaaataccag gttcgcctct tgacttcaag
      901 ttcactttaa cttgtaccat cgatttgcct gcagcgtagt cacgagattg gggattcgaa
      961 ccaacttggg gagccattcg atggaactgt gttcaaactc gggctcgaac gatcgattcc
     1021 acggcttgcg ggttgctgtc tccggaggca cgtccgggct tggccttgcg ctcgtacgcg
     1081 aattgtttga gcgaggcgcg cgggtggcat tcgtcgcccg cacgcgaaga cgcgttgagc
     1141 aggttgcgag cgaattggcg ggcgtccacg gaattgtggg cgatgtttct ttgaaaggtg
     1201 acatacaccc gatcgtcctg cagatcctgg gtgcgctcgg tggtctcgac atcttgatca
     1261 acaacgcatc ggccctcggc cctgcacctc ttgcgctgct gggcgacact gactgcgaaa
     1321 gcctggaacg ggcgttggcc accaacctgg tcggaccatt tcggttgacg aaagcgcttc
     1381 tcggcgcact gttcgcatcg gctcgtgagg gctctggcgc gctggttgtg aatgtctcga
     1441 gcaatgcggc ggtaagcgcc tacccctact ggggggcgta cggtgcgagc aaggccgcgc
     1501 tacggcactt gagcgcgatc tgggacgccg agcttgcacc cgaaggaatc caattcgtct
     1561 cgctcgatcc gggtgacatg gacactccgc tgcacgcgat cgcggtgcct gacgccgaca
     1621 gagcggccct caaacagcca caggttgcag cccgagaaat gctcgatgcg attgccgccg
     1681 cgctgcccaa ataccattcc attccaggag ccatcttatg atcgccgctg acagccccgt
     1741 ccagcgcggg ccatgggcga agctcttggt ggtcagggcg agtggggaaa tgattcacgc
     1801 ccggcgcgct gggttcgtcg acttcttgcg gccgggtgac gtggtgatcg ccaatgatgc
     1861 ggcaaccctg ccggccagcc tgaaaggccg acacgcaccg agcggcttgc ccatcgagat
     1921 acggctcgcc ggccggcgct cgctgggcac ggatgacgtc cttcagttct cagcggtggc
     1981 cttcggatcc ggcgactatc agacacgcac ggaagatcgg gctccgccgc cgcgcatggt
     2041 gcccggcgat cgactcgatt ttggggcgtt atcggcgacc gtcgagcgac tgctcggaca
     2101 cccccgcctg ttgtcgctac gattcgacgg tgcgcccgac gcggtctggt ctggccttgc
     2161 gcggcatggc cggccgatcc aatatgcgca tgtacgcatg ccactggctc tgtgggacgt
     2221 ttggacgccg atcgccgggc ttccggctgc gtttgagtcg ccgtcggccg ggttcgcact
     2281 cgactggcgg tctgtcgcgg ccatgcgggc tcgcggaatt gaattcagca ccgtcactca
     2341 cgcggccggc atctcatcga ctggcgacga cgagctcgac cgacgattac cgtttgacga
     2401 accctaccgc attccgccgt ccgccgcggc tgcgattgct cgagcgcgct cgcgcggcgg
     2461 ccgcgttgtc gcaattggta cgaccgtggt gcgagcactc gagcacgcag cgaccgtcga
     2521 ccatattgtc acggccggag cggcgctggc cacgcagcgc atcggcggcc cgacgatctt
     2581 gcaggtcgtc gatgcggtct tgtctggcat ccacgagccc ggcaccagcc attacgagat
     2641 gcttcgagcc ttttcgagcg acgagacgct tgctcgcgcg aacgacgagc ttgtcgcgcg
     2701 caattacttt acgcatgaat ttggtgattc cgttctcatc gagcgcgccc aaaacgacga
     2761 attcgtctct gattgacatg gcaatggccg taagccttgc agacgagccg agtgtcggcc
     2821 caacacgcaa ccagaattga ccattcggga gaatttccat gttggagcga tcgtcatgca
     2881 cgctcgatcc agcggactcg cgatgtgatg accgcgccga gccgagcgcc aaacatcggt
     2941 gtcagttgac gacgaaccag acctatcggt cgctcgtcta cagaccgggc gccatccgga
     3001 agcggcagat tgagctgggc gaactgtct
//
LOCUS       DATAQL010001128         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1032664, whole genome shotgun sequence.
ACCESSION   DATAQL010001128 DATAQL010000000
VERSION     DATAQL010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032664"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VHS97_19490"
     CDS             <1..242
                     /locus_tag="VHS97_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450448.1"
                     /translation="VAGIKAYRMWEELHDVAEPDLPADLLETFKEAHAAGELDDQEFE
                     RVRQRLAGPAAGGAPAMPEIRPKPDATTESDAANS"
     gene            complement(305..1351)
                     /locus_tag="VHS97_19495"
     CDS             complement(305..1351)
                     /locus_tag="VHS97_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450449.1"
                     /translation="MVGVGIVGIGFMGMIHYLAAQRAQGLRVAALCSRDSKKLAGDWT
                     SIQGNFGPRGTQMDLSGVALHRDYEALLADPQVDLVDLCVPNDAHGPMAIQALKAGKH
                     VLVEKPIALDTAQADQMVAAAQSAGKYLMVGHVLPFFPEFAYALEAVQSGRYGPLRAA
                     HLMRVISKPDWSNQIADPERSGGPAIDLHIHDTHYIGLICGVPRAVHSRGVVENGAVV
                     HLTTQYLYDKPDVAITCVSGALSQAGRPFAHGFELYFDRATLAFEFANLGGQGHTAMP
                     LTVILPDGAVEQPKLGSGDPIDSFADELATAAKAVSSGREAERLSGNLARQALRLCLA
                     EIESVKTRKVVELS"
     gene            1895..>3188
                     /locus_tag="VHS97_19500"
     CDS             1895..>3188
                     /locus_tag="VHS97_19500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX3450450.1"
                     /translation="MNRYNVVRTGIFLVAILLTGSAVSAQEDGRFSTSEAMIPMRDGV
                     KLHTRIFRPKDQRQDLPIIFLRTPYGIASSERNFVTYLKALAEEGYLFVFQDIRGKFG
                     SEGTFVMARPPRPENDDKALDEGTDTYDTIEWLLKNVSRNNGRVGMLGISYGGWTTIM
                     GALEPHPALKAISPQASPVDQWLGDDFHHNGAFRLSYGFEYASSLETGKDLADFAFDR
                     HDTFDWYLALGPLSNANAKHLHGKIPTWNDFVAHPDYDEFWKCQTPISRLRDVKVPTL
                     NVAGWWDQEDFYGPMRIYEALEKRDDKGLNFLVVGPWNHGGWSRGAGDRLGPISFDVA
                     TGKYFREEIQAPWFAYFLKDKGKLDLPEALTFEPGSNRWRRWNAWPPLKDTSERRLYF
                     TADRRLSFEAPTGGDDAADAYVSDPAHPVPYRHRPIQPT"
BASE COUNT          629 a          966 c          959 g          634 t
ORIGIN      
        1 tggttgctgg gatcaaagct taccgcatgt gggaagagct tcacgatgtc gccgagcccg
       61 atttgcccgc cgacctactc gagacgttca aagaggctca tgccgcgggt gaattggacg
      121 accaagaatt cgagcgggta cggcagcggc tcgctggtcc agcggccggc ggtgctcccg
      181 ccatgccgga aattcgcccc aaacccgatg ccacgaccga gagtgacgcc gccaattcat
      241 gaaatgtaaa gacagttttc aatggtgtgt gacgtggccc gtcagtgcca gccgtgacgt
      301 gcgttcacga gagctcgaca actttccgag ttttcacgct ctcgatttcc gccaggcaaa
      361 gtctcagcgc ctggcgtgcc aggttgcccg agagtcgttc ggcctctcgt cctgacgaaa
      421 ccgccttagc cgcggtagcg agctcgtccg cgaacgagtc gataggatcg cccgagccta
      481 gcttgggctg ctcgaccgcg ccatcgggca agatgaccgt cagcggcatg gcggtgtggc
      541 cctggccccc aagattagcg aactcgaagg ccagcgtcgc ccgatcgaag taaagctcga
      601 agccgtgggc aaacggcctg cctgcctggc tcaacgctcc tgaaacgcaa gtgatagcca
      661 cgtcgggctt gtcgtagaga tattgtgtgg tcagatggac tacggcgccg ttttcaacga
      721 cgccgcgcga gtgaaccgcc cgcggaacac cgcagatgag cccgatatag tgcgtatcgt
      781 ggatgtgcag atcgatagcc ggtccgccgc ttcgctcggg atcggcgatt tgattggacc
      841 agtcgggctt ggaaatcact ctcatcaaat gggctgctcg caacggtcca tagcgcccgg
      901 attgaaccgc ctcgagggca tacgcgaatt caggaaaaaa gggtagaacg tggccgacca
      961 tgaggtactt gccggcggat tgagcggcgg caaccatctg gtcggcttgg gccgtatcga
     1021 gcgcgatcgg tttttcgacc agcacgtgtt tgccggcctt gagcgcctgg atggccatcg
     1081 gcccatgcgc gtcgttgggg acgcacagat cgaccagatc gacctgcgga tcggcgagga
     1141 gagcctcata gtcgcggtgg agcgccacgc cggaaaggtc catctgggtg ccgcgcggcc
     1201 cgaaatttcc ttgaatgctc gtccaatcac cggccagttt cttggagtca cggctgcaga
     1261 gtgcggctac gcggagtcct tgcgcccttt gcgccgctaa gtagtggatc atgcccatga
     1321 agccgatgcc gacgatgcca acgcctacca ttgtcgttaa tcctttgatc gatgctgttc
     1381 ggagaacgcc acgatcctga agggtttcag aggtcgccgt cgtttgctgc aatcgtggat
     1441 gaagttcttc tttggcattc ttgatgcggt caacagcggg gaattgggct ttcgagggct
     1501 cagggcagcg tgattttgac cgccgttcca tacttcggca agatctgagt ggcgggccgg
     1561 caacgttgaa gcatcgcttt ctggacttgc cgggagcact cctctattgc tcgagacgct
     1621 tccgtacatc aatcgcccgt tcccggtcga ccggtttgcg cgctcgaacg acgacgcctc
     1681 cgtccttgca agccatcaag agtgccttcg tccgcccaag ttatgtcttt tccggtcgat
     1741 tttgatgacg tcggcatccg gctagaatcg gtcgccgccc accggaaagc cagtataaaa
     1801 taaagcggca acaacccgat gaccgcgggg agccttgagt ctgccgagat cgattgtcgc
     1861 cagtccgaac acgaatgggg atcttgggaa aatgatgaat cgctacaatg tcgtccggac
     1921 tggcattttc ctcgtcgcta ttctgttgac cggctcggcg gtgtccgctc aggaagacgg
     1981 gcgattcagc acgtcagagg cgatgatccc gatgcgcgac ggcgtcaagc tgcacactcg
     2041 catcttccgc ccgaaggacc agcggcaaga cttgccgatc atcttcttgc gaacgccgta
     2101 cggcatcgcc tcgagcgagc gcaacttcgt gacgtactta aaggcgctgg cggaggaagg
     2161 ctaccttttc gtcttccagg acattcgcgg caagtttggc tcggagggga cgttcgtgat
     2221 ggcacgtcca cctcgccccg agaacgatga caaagccctg gatgaaggga ccgacactta
     2281 cgacaccatc gaatggcttc tgaaaaatgt ttcgcgcaac aacggccggg tcggcatgct
     2341 tgggatttcg tacggcggct ggaccaccat tatgggcgcg cttgagcccc acccggcgtt
     2401 gaaggcgatc tcaccgcagg cgtcccccgt cgatcagtgg ctcggcgacg acttccacca
     2461 caacggcgca ttccgactga gttatggctt cgaatacgcc tcgtcgctcg agaccggcaa
     2521 agacttagcg gatttcgcct tcgaccgcca cgacacgttc gattggtacc tggcattggg
     2581 ccccctgtcc aacgccaacg ccaagcacct gcacgggaag atcccgacct ggaacgactt
     2641 tgtcgcgcac cccgattacg acgaattctg gaagtgccag acgcccatct ctcggcttcg
     2701 tgatgtgaag gtaccgacct tgaacgtcgc gggatggtgg gatcaagaag atttctacgg
     2761 ccccatgcgc atctacgagg ctctcgaaaa gcgcgacgac aaaggactca acttcctcgt
     2821 cgttggaccg tggaaccacg gcgggtggtc ccgcggggcg ggcgaccgcc tgggccccat
     2881 cagtttcgac gtcgccaccg ggaagtattt ccgcgaggaa atacaggccc cttggtttgc
     2941 ctatttcttg aaggacaagg ggaagcttga tctccccgag gccctcacgt tcgagccggg
     3001 gtcgaaccgc tggcggcgct ggaacgcctg gcccccgctg aaagacacat ccgagcgccg
     3061 gctctacttc accgcggaca ggcgactgtc gtttgaagcg ccgaccggcg gcgacgacgc
     3121 ggcggacgcc tacgtgtccg accccgccca tccggttcct tatcgccatc ggccaatcca
     3181 gccgactt
//
LOCUS       DATAQL010001129         6134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_876470, whole genome shotgun sequence.
ACCESSION   DATAQL010001129 DATAQL010000000
VERSION     DATAQL010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876470"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VHS97_19505"
     CDS             <1..780
                     /locus_tag="VHS97_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX3450451.1"
                     /translation="IEAVIVLSPATDLPVLGKVAAYAQLSPANVKLELVAGTIIDCGK
                     RVREHYVPLGYFNVATFQEPGWRTEGKKTLGLEMAEPAGDREADRRWQLPDVIVYPTG
                     GGTGVIGMAKAFDELEALGLVGSQRPRMVCVQSDASTPIVRAFESGAMDIEPLPPGST
                     IATGLNVAQNVGHINVLKIIRETGGCALAVSEEAIGQTIRDEWRERRFAWSPEGAATV
                     AALPELIDRGIVGPKDRVVLVNTASAEKYLPSIRSLLDGGL"
     gene            complement(881..1645)
                     /locus_tag="VHS97_19510"
     CDS             complement(881..1645)
                     /locus_tag="VHS97_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEX3450452.1"
                     /translation="MRILVVSDIHANWAALTAINQPFDVCLSLGDLVDYGPDPAPCVR
                     WAMEHATYAIRGNHDHGVAQGIPVTGSNGFRYLTQVTRPSMWGALGPEERRYLLQLPV
                     TQRFTLEGKRFLLVHATPRDPLDEYLLKDPEMWAKRLQNVDADVVCVGHTHLQFNIQV
                     NGVLVLNPGSVGLPRDGDPRAGFAIIDDNKIELKRVNYPVADTIARIEEMPWPQRGKD
                     LMSYVLRYGYLPQHLDHDGTAAALSPESDPDNGDDD"
     gene            complement(1655..3532)
                     /gene="asnB"
                     /locus_tag="VHS97_19515"
     CDS             complement(1655..3532)
                     /gene="asnB"
                     /locus_tag="VHS97_19515"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX3450453.1"
                     /translation="MCGIAGFVNRLGQAADRGIVERMTATLAHRGPDGDGIYCEGPVA
                     LGHRRLSIIDVAGGAQPMTNEDGTIWVSYNGELYNELELRKELEAKHHRYRTTSDTES
                     LVHLYEEEGPDFVRRLNGMFALAIWDSRRDRLVLARDQMGQKPLYYGSLPGGGLAFGS
                     EPKALLRHPDIGRDLDRESLARYLFFEYVPAPYSIWRSLRKLASGHVLTWERGEFRVE
                     KYRTLPVMGSSSAPKLGASAERLWSGLRDSVARHRRSDVPIGVFLSGGIDSSSVAAAL
                     CEIESPRNVRTFSIGFEDPSFDESGHARTVARHLGTDHHERTFSVKSAYELLPEVAAW
                     LDEPFGDASILPTHLLSRFAREQVKVILGGDGADELLAGYPTFKAEHAAGLFRRLPRP
                     AQALAGAAVGRMPVNHDNFSLDFKLKQFLRGAAEPLPLAHQRWLGSFSGAEINHLLVD
                     GDPIGVEREHLRRSETLEGCNDPLSCSLALYQDTYLPEDILTKVDRASMACGLEVRAP
                     FLDTELVDFIQAMPPSFKFGRNQTKRVLKRAAASRLPASILARPKKGFGIPVANWLRG
                     PLAPLLGRLLDRDRLTRQGLFRPEVVARRIAEHQSGHRDHRKPLWTLVMFQLWCDNWW
                     G"
     gene            3822..5324
                     /locus_tag="VHS97_19520"
     CDS             3822..5324
                     /locus_tag="VHS97_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3450454.1"
                     /translation="MASFNPQDHGFDEPPPRPTTPPVRRGFLLVLFVLSLAALVVYGV
                     PYVAERTGYAWEAGRARADSEALARLDEAGIVNRASALFRMATAAVSPAVVNVQSYRT
                     RRGGEGFQGLPVGGGMTPGFQSSELGSGVIIDKAKGYVVTNYHVVKEADRIMVRLGPG
                     NDVRARLVGADPKSDLAVLRIKADLKVQAQWGDSEQLDTGEWVLAIGSPLGFDHSVTA
                     GIVSATERNGISEYESFIQTDAAINLGNSGGPLVNLAGKVVGINTAMFTQSGGFEGIG
                     LAIPSSQARRVVERLIKDGKVVRGYLGVRLDLRPLDAKLAKNLNLPNNRGALVLGVQP
                     GSPAEQAGLKADDVIVKLADREVADPAGLRNLTASLDAGAQVPLTFYRGGEPQTATVT
                     IAELPPAPEILARLGFNVHELRLDNEGHGTCVEIDSVIPGSPAFHKGLLPRMRILAVG
                     TNPVPVTTLAEFETAVRKTDLSRGLPMVVQYLDGRRGGVVFEFGKPKTQP"
     gene            5923..>6134
                     /locus_tag="VHS97_19525"
     CDS             5923..>6134
                     /locus_tag="VHS97_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX3450455.1"
                     /translation="MPRHSETTLAAIKNAVDIVALVGEYTPVRRMGSKYKALCPFHDD
                     HNPSLDINPDRQSYKCWSCGAGGDVF"
BASE COUNT         1218 a         1869 c         1898 g         1149 t
ORIGIN      
        1 atcgaggccg tcattgtcct gtcacccgcc accgacctgc cagtactcgg aaaagtcgct
       61 gcatacgctc agctatcccc cgctaacgtc aagctcgaac tggtcgcggg tacgatcatc
      121 gactgcggca agcgtgtgcg agagcactat gttcctctgg gctatttcaa cgttgccaca
      181 ttccaggagc caggctggcg cacggaaggg aaaaagacgc tggggctcga gatggccgaa
      241 cccgccggcg accgcgaagc ggatcgtcgc tggcaactcc ccgacgtgat tgtctatccc
      301 accggaggcg ggaccggcgt gatcggcatg gccaaagcct tcgacgagct cgaagccctc
      361 ggattggtcg gctcccaacg cccgcggatg gtgtgcgtcc agagcgacgc cagcacaccc
      421 attgtccgcg catttgagtc cggggcgatg gacatcgaac ccttaccccc cggaagcaca
      481 attgccacgg gactgaacgt ggcgcagaat gtcgggcaca tcaacgtgtt aaagatcatt
      541 cgcgagaccg gcgggtgtgc ccttgccgtc tccgaggagg cgattggcca aaccattcgc
      601 gatgagtggc gcgagcgacg gttcgcctgg tcgccggaag gcgccgccac agtcgccgcg
      661 ctccctgagc tcatagaccg aggaatcgtc ggccccaagg atcgagtcgt cctggtcaac
      721 acggcctcgg ccgagaagta tctgccgtca atacgctctc tgttggatgg cggactttga
      781 agaattcacg attgatatat ctaaggtcga gcagaccacc gcgcggcgac cgccgcgagc
      841 cccctcgcgg atgggtagct cggccttcgc gaactcaaac ctaatcgtcg tcgccattgt
      901 ccggatcgga ttccggggac aatgccgctg ctgtcccatc gtgatccaga tgttggggca
      961 ggtaaccata ccgcagcacg tatgacatca agtccttgcc gcgctggggc cagggcattt
     1021 cctcgatgcg ggcgatcgtg tccgcgacgg gatagttcac tcgcttcaac tcgatcttgt
     1081 tgtcgtcgat gatggcaaac ccggcgcgcg ggtcgccgtc gcgcggcaaa cccacgctgc
     1141 cggggttcaa gaccaacacg ccgttcacct ggatgttgaa ttgaagatgg gtatggccga
     1201 cgcagacgac gtcggcgtcg acgttctgga gccgtttcgc ccacatttcc gggtctttca
     1261 gcaagtattc gtcgagcgga tcgcgagggg tcgcatggac caggaggaac cgtttccctt
     1321 ccagcgtgaa gcgctgcgtc acaggcagtt gcaacagata gcgccgttcc tcgggcccaa
     1381 gtgcccccca catcgacggc cgagtcacct gggtcaggta gcgaaacccg ttcgagccgg
     1441 tcaccggaat cccctgggca acgccatggt cgtggttgcc gcgtatcgca taggtggcgt
     1501 gctccatcgc ccagcgtacg caaggcgcgg ggtcaggtcc gtagtcgacc aggtcgccca
     1561 gactcagaca gacgtcaaag ggctggttga tcgccgtcag ggcggcccag ttcgcgtgaa
     1621 tatcggaaac gaccagaatc ctcatgtcgc ttgatcatcc ccaccagttg tcgcaccaaa
     1681 gctgaaacat caccaaagtc caaagcggtt tgcgatggtc gcggtggccc gattggtgtt
     1741 cggcaattcg gcgggcgact acctctgggc gaaagagtcc ctgccgcgtc agacggtcgc
     1801 ggtccagaag ccggcccaaa agcggagcca acggcccgcg tagccagttg gcgaccggaa
     1861 tgccgaagcc cttcttggga cgcgccagta tcgatgcggg cagccggctc gcggcggcgc
     1921 gtttgagaac gcgcttggtc tggttgcgcc cgaacttgaa cgacggcggc atggcctgga
     1981 tgaagtcgac cagctcggta tcgaggaacg gtgctctgac ctccagtcca caagccatgc
     2041 tggctcgatc aaccttggtg aggatgtctt cgggcaagta ggtgtcttgg tacaacgcca
     2101 acgagcacga cagcggatcg ttacagcctt cgagggtctc tgagcggcgg aggtgctcgc
     2161 gctcgacgcc gatcggatcg ccgtcgacca gcaagtgatt gatctccgcg ccagaaaacg
     2221 agcccagcca gcgctggtga gccaagggaa gtggctcggc cgcgcctcgt agaaactgtt
     2281 tgagtttgaa gtcgaggctg aagttgtcat gattcactgg catccggccc acggccgcgc
     2341 cggccagcgc ctgggccggt cgagggaggc ggcggaaaag gcccgcggca tgctcggcct
     2401 tgaacgtcgg atagcccgcc aacaactcgt cggcgccgtc gcctcccagg atcaccttga
     2461 cctgttcacg cgcgaaccgg cttagcaggt gagtcggcag aatcgacgcg tcgccgaacg
     2521 gctcgtccag ccacgccgcc acttcgggca agagttcata ggccgacttg accgagaaag
     2581 ttcgctcatg atggtccgtg cccaggtggc gagcgaccgt gcgggcatgg ccgctctcgt
     2641 cgaagctcgg atcctcaaag ccgatcgaga acgtccggac gttgcgcggc gattcgatct
     2701 cgcacagggc ggcagcgacg ctcgaggagt cgatcccacc cgatagaaag accccaatgg
     2761 gaacatccga ccggcggtgg cgcgcgaccg agtcgcgcaa cccgctccat aaccgttccg
     2821 cgctggcacc gagctttggc gctgagctcg aacccatcac cggaagcgtc cggtacttct
     2881 ccacgcgaaa ttcgcccctc tcccacgtca agacatgccc gctcgcgagc ttccgtagtg
     2941 agcgccagat cgagtaaggg gccgggacgt attcaaaaaa aaggtatcgg gcgagactct
     3001 ctcgatcgag gtcgcggccg atatccgggt gacgaaggag ggccttgggt tccgatccaa
     3061 atgcgaggcc cccaccggga agggaaccgt aataaagagg cttctggccc atttggtccc
     3121 gcgccagaac cagccggtcc cgtcgactgt cccagatcgc cagcgcgaac attccgttca
     3181 ggcgccgaac gaaatccggt ccttcctctt catagaggtg aacaaggctc tcggtgtcgc
     3241 tggtggttcg ataacgatga tgctttgcct cgagctcttt cctcaactca agctcgttat
     3301 aaagctcgcc gttgtagctg acccagatcg tgccgtcctc gttggtcatc ggctgtgcgc
     3361 cgcccgcaac gtcgatgatc gacaaccggc gatgcccgag cgccacgggc ccttcacagt
     3421 aaataccgtc gccgtccggc cctcggtgcg ccagcgtggc cgtcatgcgc tcgacaatgc
     3481 cgcggtcggc cgcttggccg agacggttca cgaatccggc gatcccgcac attcggatgg
     3541 gtgctttttg gagattggcg tgctgtcaaa acctgccgcc agagcgctgt cgggcttcga
     3601 cggcataaag gaattccatg ggttggacct ggattccatg atagtaaatt gtagccgcca
     3661 caacactagc aactgccgag gccgctttgt gtcacggttg atttcgatct cgtaacgtat
     3721 aacaataatc gagcgcccgc acggggccgt tcgacgctct acgtggagag gtgccgctcg
     3781 taagtgcctc gccctcaggc tggctgggag tttcgcgact gatggcctca ttcaaccctc
     3841 aggaccacgg ctttgacgaa ccgccgccgc gtcccacgac acccccggtc cggcgggggt
     3901 tcttgctggt gctgttcgta ctcagcttgg ccgcgctggt tgtctatggc gtcccttatg
     3961 ttgcggagcg taccggttat gcgtgggagg ctgggcgggc acgggccgat tcggaagcat
     4021 tggcgcggct cgacgaggcc gggatcgtca atcgcgcgtc cgcgctgttt cgcatggcga
     4081 cagccgccgt atcgcccgcg gtggtcaacg tgcaatcgta tcgaacgcgg cgaggcggtg
     4141 aagggtttca aggccttcct gttggcggcg gcatgacacc gggtttccag agttctgagc
     4201 tcggttccgg cgtgatcatc gacaaggcaa aaggctacgt cgtgaccaac taccacgtgg
     4261 tcaaagaagc cgatcgaatc atggtccgcc tcggcccggg gaacgatgtc cgcgcacgct
     4321 tagtcggagc cgaccccaag agcgacctgg cggtcctgcg gatcaaggcc gatctcaagg
     4381 tccaggccca gtggggcgat tcggaacagc tcgacaccgg cgagtgggtc ctggccatcg
     4441 gtagcccgct cggcttcgat cactcggtca ccgcaggtat cgtctctgcg accgagcgca
     4501 acgggatctc ggaatacgag tcgttcattc aaaccgatgc ggcgatcaat ctggggaact
     4561 cgggagggcc gctcgtgaac ctcgccggca aggtcgtggg aatcaatacg gccatgttca
     4621 cccagtcggg cggcttcgaa gggatcgggc ttgccatacc ctcgtcgcag gcgcgccgag
     4681 tagtcgaacg cctgatcaag gatggcaagg tcgttcgagg ttatctgggt gtcaggctcg
     4741 atctcaggcc tctcgacgcc aagcttgcca agaatctcaa tcttcccaac aaccgcggtg
     4801 ctctcgtttt gggagttcag ccaggcagcc cggccgagca ggccggactg aaggccgacg
     4861 atgtgattgt caagctcgcc gatcgcgagg ttgccgaccc ggccggactc agaaatctga
     4921 ccgccagcct ggatgccggc gcccaggtcc ctctcacatt ctatcgtggc ggcgagcccc
     4981 agacggcgac cgtcaccatc gccgaacttc cccccgcccc cgagatcctc gcgaggttgg
     5041 gtttcaacgt ccacgagctg cggctcgaca acgaggggca cggaacctgc gtcgagatcg
     5101 atagtgtgat tcctggcagc ccggcgttcc acaaaggcct gctacccaga atgcgaatcc
     5161 tggctgtggg aaccaacccg gtgcccgtga cgacattagc cgaatttgag acggccgtca
     5221 ggaagaccga tttgagccgc ggcctgccga tggtcgtgca gtatctagat ggcaggcgcg
     5281 gcggtgtcgt cttcgagttt ggcaagccga agacgcaacc ctgaacacac cgatcttccg
     5341 ccacgagttc tttcgacgcc tacttcagtt ggggtgcatt gaacgaccgt gccctgaccg
     5401 aaggaatcga gcacccccac ggtatccacc atccgtggcg aatcgagacg tcgatccgcg
     5461 ggaattcacg cctctgcaat cgcgtgcaga tcggtcttgt gaaaaataac aagaaaacga
     5521 aaaatttggt acttgacttg gacatttggt ttacaggaca tgaattagcc atcgatttcg
     5581 agattatgag gatatggata aggtccatgc ccggttgagc gcgggtgttc ggcgattggg
     5641 cctggagggt gagcgcggat ggaggagaac ggcctgggta cggcccgacc ccggcaaaca
     5701 atacctaact gctaaaatcg tgccgtgtta gaaagagggc caagttgtcg gccgatcgcc
     5761 tggaaaactt gaccggaaat agtcgagggt tataattcac gtgaagccgc gatgatcaaa
     5821 tagggcacgg caagcatcct cggtgggtgg gcagatagct gtccgccctt ggggtgtgcg
     5881 aaccatggac ccgggcctga gcccttaagt caggagtccc cggtgccccg tcattccgaa
     5941 acgacactag ccgccataaa aaacgcggtc gacatagtcg ccttggtcgg cgaatatact
     6001 cccgtgcgcc gcatggggag caaatataag gctctctgtc cgttccacga cgatcataat
     6061 ccgtcgctcg atattaatcc ggatcgccag tcatataaat gctggagttg cggagcgggc
     6121 ggcgatgtat tcga
//
LOCUS       DATAQL010001130         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2557295, whole genome shotgun sequence.
ACCESSION   DATAQL010001130 DATAQL010000000
VERSION     DATAQL010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557295"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..489
                     /locus_tag="VHS97_19530"
     CDS             262..489
                     /locus_tag="VHS97_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450456.1"
                     /translation="MCNDYRLNVEASAIPDDFAKLKIKVWSSEGAPTIQPRDDIKIMD
                     TIVGWWTATIAPVTSFSGAGVGRRPRGTLLQ"
     gene            1153..2508
                     /locus_tag="VHS97_19535"
     CDS             1153..2508
                     /locus_tag="VHS97_19535"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3450457.1"
                     /translation="MNDASPRFAEDQAGAASLSSSEERNLPLAELAQHAVGLNAWVTE
                     EGLRGATAGELFDGYCQRLSAMDVVIMRAYVSTQTLHPQWTGYGYTWRRELRSVREQQ
                     FARGPISQEWLSSPFNALIQRSLAGEKNPWLRRRLELGPDQRDFPALADFYAAGPDCV
                     CLAFRFGEAGDPSHGTGVLYSFTTDRPGGFRTAEIDLLRSTLPGLSLAMKAHTGHDIA
                     SGLLRTYLGHDAGSRVHSGAVERGTVDGLRSVLWYADLRGFTRISDVSSGAAIVEMLN
                     DTFETLTAALRPRGGHVLKFIGDAVLATISFDEPDEKATCRRALDAAVEASANLNIRN
                     LEREAARIPFADVDIALHVGEVLYGNVGAVDRLDFTVIGPAVNGVVRMEKLCEPLGQH
                     ILFSSRFAEAAGRCDGRLESLGHFQLRGVDEAKEIFGLRLSPLCNAWKPDEIIVRPER
                     G"
     gene            2773..2919
                     /locus_tag="VHS97_19540"
     CDS             2773..2919
                     /locus_tag="VHS97_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450458.1"
                     /translation="MQWQKARHPNLTIELRIANWTRAVADLLEGAVELASRRVRRQRL
                     GTLL"
     gene            3024..3341
                     /gene="groES"
                     /locus_tag="VHS97_19545"
     CDS             3024..3341
                     /gene="groES"
                     /locus_tag="VHS97_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX3450459.1"
                     /translation="MKFRPLHDRVVIRRIEGEDKTKGGIVIPDTVKEKPQEGEVIAVG
                     PGARDESGKLVPLDLKAGDRVLFGKWSGTEVKIDGEDLLIMKESDVMGVIEGATATKK
                     KAA"
BASE COUNT          744 a         1000 c         1099 g          692 t
ORIGIN      
        1 aatgcgatga cgacatgata ggtcactggc aatttcagac cggcttcaaa ttggggacgg
       61 gaggcgggtg gcggtcgcgc gatggctgaa gccgctaagg gtgagaaacg attacggtcc
      121 acgcgcctag ccgggtttga agttgcgcgc aacaaccccg gctcacgtca tcgtctcgat
      181 aatgtgacag cgcccaggca actctccaat gtcgcggccc tgatctagct ttcccggcac
      241 tagtcctcgc cggagagcct catgtgcaac gattaccgat tgaatgtgga ggcgtcggcg
      301 atccccgacg atttcgcgaa acttaagatc aaggtctggt cctcggaagg tgcacccacc
      361 atccagccgc gtgacgacat caagatcatg gacacgatcg tcggatggtg gacggcgact
      421 atcgcgccgg tgacctcgtt cagcggtgct ggagttggcc ggcgacctcg gggaacgctg
      481 ctgcaatagc cccctctggc ggatgtgatg gcatgaggaa ggcaccggcc gggcttgctc
      541 gcaaacgcgt ggtccaagga ttatatctgt gtggggagag aaaggcagca atcgtcgact
      601 gatcaggcgc gagagcgctg acagcgcgaa tgaaaaggtt gcgaaaccaa aatggccaaa
      661 atagccggcc ctatcaaacg accgacatcc aacggcgggt tccgcggtga tcaggaggcc
      721 cctcaaccca tgatcaatcg ggagcgtcgc aataaatcgg agccgctcgc cgcgtcagtt
      781 caggttaaat ttatggagct tcgaccgagc aagtgtcgct ggccgatcgg cgacccgaaa
      841 catatcgaaa cattccggtt ttgcggttcc gcgtgctcgt ctggagctat ctattgtaaa
      901 gcgcacaccg caaaagccca cgctccaaac cgcccaagga cgccacgtac gacaaatttt
      961 cagttgcgag ttagagtccc gtgacagcaa cagttctgtc gcgggcagag aagccacgaa
     1021 gccagcccac atcgtggatc gcgctacagg ctagccgtac ggacgtcgct tattacgtaa
     1081 gggtccgtaa gttgcggcat cctcactcgc tgcagtgtgt ggagggcggc gaccaagcgg
     1141 aggttcaaaa aaatgaatga cgcatcgcct cgctttgccg aggaccaagc gggcgcagca
     1201 tcattatcat cgagcgaaga gcgcaacctc cccttggccg aactcgctca gcacgcggtc
     1261 gggctgaacg catgggttac ggaggaaggg ttgcgaggcg cgacggcagg cgagctcttc
     1321 gatggatatt gccaacgatt gtcggcgatg gacgtcgtca tcatgcgtgc atacgtgtcg
     1381 acccaaactt tgcaccccca atggactggc tacggctaca catggcgacg agaactaaga
     1441 tcggtgcgtg agcagcagtt tgctcggggc cccatttcgc aggagtggtt gagtagcccc
     1501 ttcaatgcgc ttatccagcg atcactcgct ggcgaaaaga atccttggtt gcggcgccgg
     1561 ttggaactcg gcccagatca acgagacttt cccgcgctcg cagacttcta tgccgccggg
     1621 ccagactgcg tgtgtctagc ctttcggttt ggagaggcgg gtgatccgtc gcacgggact
     1681 ggcgtccttt attctttcac gactgatcgc ccagggggct tccggacagc agaaattgat
     1741 ttgttgcgtt ctacgcttcc ggggttgtcg ctcgcgatga aggctcatac ggggcacgat
     1801 atcgcgtcag gactcctacg gacttacctt ggacatgacg cgggttctcg ggtgcactca
     1861 ggcgcggtcg agcgtggaac ggtcgacggc cttcgctcgg tgctttggta cgctgatttg
     1921 cgcggtttca cacggatcag cgatgtgtcg tccggggcgg caattgttga gatgttgaac
     1981 gatacttttg agacattgac cgccgcgttg cggccgcgcg ggggtcacgt gctcaaattt
     2041 atcggcgatg cggtgctagc gacgatctcg ttcgatgagc cggacgagaa ggctacctgc
     2101 cgccgcgctc tcgatgctgc agttgaggcg tcggccaact tgaacattcg caacttggaa
     2161 cgcgaagcgg cacgtatacc gttcgctgat gttgatatcg ctttgcacgt cggcgaagtg
     2221 ctgtacggca acgtcggcgc ggtggaccgg cttgacttca ccgtgatcgg acctgcggtc
     2281 aatggggtgg ttcgaatgga aaagctctgt gaacctcttg gccaacacat cctgttctca
     2341 tctcgattcg ctgaagctgc gggacgctgt gacggacgcc tggagtcgct cggacatttt
     2401 cagttgcgcg gcgtcgatga ggccaaggag atttttggcc tacggctctc gccgctttgc
     2461 aacgcgtgga agcctgacga aataatcgtc cggccagaga ggggctgaca agactcgctg
     2521 agctcgtccg tccacacctt gaaactgact agcgtattgg gaccgagctg tgcgtgatag
     2581 gccgtcggcc gcgtcgcgtg cgcgatcagt acgcggccta tttctcgacg ggttattgcg
     2641 cgggtcgaag gtgtatcact gcccgcgcag gaaggcttcg aaccagccgg cgaaatccat
     2701 tgggcctagg gcagcggcac accaatatca atatctccac gagagccggt ggaataccgg
     2761 ccggagtgtt tcatgcagtg gcagaaagca cggcacccca acctcaccat cgagcttcgg
     2821 atcgccaact ggacccgtgc ggtcgcggac ttgctcgagg gcgcggtcga attggcgtca
     2881 cgtcgagtgc gtcggcagag gcttggcact ctcttgtaac gagtgccaac ggcagctatc
     2941 tacgtcagac ttgagactgc gagccgaacg ccagggcgtc tcgctcgagg ccggcatcgc
     3001 aggtcaaacc gggagcgaaa ctcatgaaat tccgtccgtt gcacgaccgc gtcgtcatcc
     3061 gccgcatcga gggagaggac aagacgaaag gcggcatcgt cattcccgac accgtgaagg
     3121 aaaagcccca agaaggcgag gtcattgccg tgggcccggg cgctcgggac gagagcggca
     3181 agttggttcc gcttgatctc aaagcgggcg accgggtgct gttcggcaaa tggtccggca
     3241 ccgaggtcaa gatcgatggt gaggaccttt tgatcatgaa ggagtccgat gtcatgggtg
     3301 tgatcgaggg cgcaaccgct acgaagaaaa aggcggcctg accccgcccg cccctcatct
     3361 caaaccaggg agtgaaaccc atgtccgcca aagacgtacg cttctcaacc gacgcccgcg
     3421 accgattgct gcgcggcgtc gatattctca ccaacgccgt caaggtgacg ctcggtccga
     3481 agggccgcaa tgtcattctc gataagtcat acggcgcgcc gcgcatcacc aagga
//
LOCUS       DATAQL010001131         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_876746, whole genome shotgun sequence.
ACCESSION   DATAQL010001131 DATAQL010000000
VERSION     DATAQL010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876746"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VHS97_19550"
     CDS             <1..295
                     /locus_tag="VHS97_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450460.1"
                     /translation="GDGSGAIELTTGRLERGDGRRLSRDEGVAKPSDPDSTRKEVTTG
                     VKPDHAIGQSGARARRFSPPRSSRAPDQEPNLASDADVQEAVRLLQKLVQAII"
     gene            309..2129
                     /locus_tag="VHS97_19555"
     CDS             309..2129
                     /locus_tag="VHS97_19555"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX3450461.1"
                     /translation="MRTRLMTKRPPFRLFVLFWLTALQFHSAPREGIAGDTPVDTDPL
                     ANRMRQPVALAIAEDGKSLFVANRRSGSLSVIDTTRRKVVAEHDFGRGLADVAILAGG
                     RHLLAVDQEANELLLIDGRDRSIRVVDRIKVSPDPIRLVVLADGPSCVLASLWSRRLT
                     FVSLERRDPVDIHPVLAITARLDLPFCPREMAVVADGSRLIVADAFGGRLAVVDFKQR
                     TIESVRTLPAHNIRGLAVAPDGKTLVVAHQVQSRLAQSSFDDVHWGLLIRNHLRILQI
                     DTLLRPGPDAALLDSGRLFDLGDVGYAAGDPGDLAFDARGNLIVALAGVDEVAITASP
                     DQGPRRIVVGRQPTAVIPSPDGSLVYVADSLDDTISVVDIASGLRPATIALGPRPDLT
                     SRDRGERLFSSAKLSHDGWMSCHSCHSDGHTNNLLSDTLGDGSYGAPKRVPSLLGVAA
                     TGPWTWTGSVARLDDQIKKSIVTTMHGPKPSDEQVSDLAAYLTSLAPPSPRQVDIDPL
                     DASAVSRGREVFATRKCAICHVAPEYTSPERFDVGLTDEVGNREFNPPSLRSVSRRDA
                     LLHDGRTHSLEEVFRKERHPRGLALSPQEIADLVAFLKTL"
     gene            2309..>4074
                     /locus_tag="VHS97_19560"
     CDS             2309..>4074
                     /locus_tag="VHS97_19560"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX3450462.1"
                     /translation="MSRTRRVHPDRFATRSSAALCLSIVSLICNFESRAAEPSSPKKR
                     TVDFNREVRPILAKNCFACHGQDEAKRAKGLRLDQRESAVRPLKSGETAIVPGDADSS
                     ALFLRVTEEDDTLRMPPRKAGNRLTSSEAEVLRSWIEQGATYASHWALVAPTSDSYPA
                     VRNKAWPRNGIDMWTLARLEDAGLTPAPEADRATLLRRVSFDLRGLPPTADEFEQCIH
                     DKDPNAYEKAVDRFLVDPAFGERWARMWLDQARYADSAGYGSDPLRPNIWPYRDWVIR
                     AFNHNVPFDEFTIDQIAGDLLPDPPLEAKIATAFHRNTMTNTEGGTDDEEFRVAAIKD
                     RVDTTMQVWMGLTMGCAKCHSHKYDPITHAEYYQFYAFFNQTADADQPAETPVIPVPS
                     PQSLAQIKRIDAEITELRQTLEACTAELSTAQAKWELSLGPAGSAARKGLPKEIQTIL
                     DTAADQRTTPQTETLQRHFRSNAPELKPVRDKIAALEKSKPIIPSVPVMVELPADHRR
                     VTRILKKGNFLDPGDEVKPGVPQSLHRLAADAPKNRLGLARWLVDSANPLTARVAVNR
                     FWAQIFAAGLVETEEDFGTQGEP"
BASE COUNT          785 a         1299 c         1211 g          779 t
ORIGIN      
        1 cggcgacggt tcgggggcga tcgagctgac gacgggacgc ctggaacgcg gcgatggccg
       61 acggctcagc cgcgatgagg gcgtcgctaa gccatctgat cccgattcca cacgcaaaga
      121 ggtcaccaca ggagttaagc ccgatcatgc tatcggccag tccggagcac gggcccggcg
      181 gttctcaccg cctcggtctt cgcgagcgcc agatcaagag ccgaatcttg ctagtgacgc
      241 agacgtccag gaagcggttc gattattgca gaaattggtt caggctatca tatgacgcga
      301 gttcttcgat gcggactcga ctgatgacca agcggccgcc gtttcgactt ttcgtcctct
      361 tctggctgac ggcgttacag tttcactcgg cgccgagaga gggtatcgcg ggggacacac
      421 ccgtcgacac cgatcccctc gcgaaccgca tgcgccaacc agtcgcgctc gccatcgccg
      481 aggacggcaa gagcctgttc gtcgccaatc gccgcagcgg gagcctgtcg gtcatcgata
      541 cgacgaggcg aaaggtcgtt gccgagcacg attttggccg cggactggcg gacgtggcga
      601 tcctcgccgg cggccggcac cttctcgcgg tggaccaaga ggcaaatgaa ctccttttga
      661 tcgatggtcg agatcgatcg attcgagtcg tcgaccgcat caaggtcagc cccgatccga
      721 ttcggctggt cgtcttggcc gacggacctt cgtgtgtatt ggcctcgctc tggtcccgtc
      781 ggctcacgtt cgtcagtcta gagcggcgtg accccgttga cattcatccg gtgcttgcga
      841 tcaccgccag actcgatctg cccttctgcc cacgtgagat ggccgtcgtc gccgacgggt
      901 cgaggttgat cgtcgccgac gcgttcggcg gtcggctggc tgtcgttgat ttcaaacaac
      961 gaacgatcga atcggttcgg acgctgccgg cgcacaatat ccgaggactg gctgtcgcgc
     1021 cggacggcaa gacgctggtc gtcgcgcatc aggtacagag ccgcctggct cagtccagct
     1081 ttgatgacgt tcattggggg ctcttgattc ggaaccatct acgaatcttg cagatcgaca
     1141 ccctgttgag acccggtccc gacgcggccc tgcttgacag cggtcggctg ttcgaccttg
     1201 gcgatgtcgg gtatgccgcg ggcgacccgg gcgatcttgc gtttgatgcc cgcggcaatc
     1261 tgattgtggc actggccgga gtggacgagg tcgcaatcac agcgagcccc gaccaaggcc
     1321 cgcgccgcat tgtcgtcgga cgtcaaccga ctgccgtgat accgagcccg gatgggtcgt
     1381 tggtttacgt ggccgacagt ctcgacgaca cgatctccgt ggtcgatatc gcctctggct
     1441 tgcgccccgc cacaatcgct ctgggaccgc gccccgattt gacctccagg gaccgtggcg
     1501 agcggctgtt ttcgagcgcc aagctgtctc acgatggctg gatgagctgt catagctgcc
     1561 acagcgacgg ccacaccaac aatctcttga gcgacacgct cggcgacgga tcgtatggcg
     1621 cacccaagcg tgtcccgtcg ctgctgggag tcgccgcaac cgggccctgg acctggacgg
     1681 gctcggtcgc tcgcttggac gaccagatca agaagtcgat tgtcacgacc atgcacgggc
     1741 ccaaaccgag tgacgaacaa gtgtctgact tggccgccta tctcacttcg ctcgcgcccc
     1801 catctcctcg gcaagttgac attgaccctc ttgacgcgtc tgccgtttcc cgcggtcgcg
     1861 aggtgttcgc tactcggaaa tgcgcgattt gccacgtcgc tcccgagtac acttcgcccg
     1921 aacgttttga cgtgggattg accgacgagg tgggaaatcg tgagttcaac cctccttcac
     1981 ttcggagtgt gagcaggcgc gatgctctcc tgcacgatgg acgcacccat tcgctcgaag
     2041 aggtttttcg gaaagagcgg catccccggg gcctcgcgct cagtccccaa gaaatcgccg
     2101 acctggtagc gtttctcaag accctctagg ctgcgttttc gtctccagcg tattgagccc
     2161 gggcttgaga tcaacgatga tatagagtga ggtgatgcga gaagacgttt catacccgcc
     2221 atgaagattg cacggaccac acctgcctga atcccagccg attcccgttc gaccgaccga
     2281 cgctattgtt gctgggggag cccaatcgat gtccaggacc cgccgcgttc acccagaccg
     2341 atttgcaacc cgcagctctg cggctttgtg cctctctata gtcagcttga tctgcaattt
     2401 tgagtcacgg gcggccgagc cgagctctcc aaaaaagcgc acggtcgatt tcaatcgtga
     2461 ggttcggccg atcctcgcca aaaactgttt cgcgtgccac ggtcaagacg aagcgaagcg
     2521 tgccaagggt ctgcgccttg accagcgtga atcggccgtc aggccgctca agagcgggga
     2581 gacggcgatt gtgcccggcg acgccgattc cagcgcgcta ttcctcaggg tcacagagga
     2641 agacgacact ctgcgaatgc cccccaggaa ggcgggcaac cgactgacgt cttccgaagc
     2701 cgaggtcttg cggagctgga tcgagcaagg ggcaacatac gcctcccact gggcgttggt
     2761 cgctcccacg tccgattcct accctgctgt ccgcaacaag gcatggccgc ggaacggcat
     2821 cgacatgtgg accctcgccc ggcttgagga cgccgggctc acccccgcac ctgaggcgga
     2881 ccgcgcgact ctgttgcgcc gcgtcagttt cgacctccgc gggttgcccc ccacggccga
     2941 tgaattcgag cagtgtatcc acgataaaga tcccaacgcc tacgagaagg ccgtcgaccg
     3001 ctttcttgtt gaccccgcct tcggtgagcg ttgggcacgg atgtggctcg atcaggcacg
     3061 ttacgccgac tcggccggat atggctcgga cccgctgcgg cccaatatct ggccgtatcg
     3121 cgactgggtc atccgggcct ttaatcacaa cgtccccttt gacgaattca ccatcgacca
     3181 gatcgccggc gacttgctcc ctgatccgcc gctcgaagcg aagatcgcga cagctttcca
     3241 ccggaacacg atgaccaaca ccgagggggg aaccgacgac gaagaattcc gcgtcgccgc
     3301 catcaaggac cgagtggaca ccacgatgca ggtctggatg ggcctgacga tgggatgcgc
     3361 caaatgtcat agtcataagt atgatcccat tacacatgcg gaatattatc aattttacgc
     3421 attcttcaat cagacggccg acgcggatca gcccgccgag accccggtca ttcccgtacc
     3481 aagcccgcaa agtctggcac agatcaagcg gatcgacgcc gaaatcactg aattgcgcca
     3541 gactctcgag gcgtgcacag ccgagttgtc cacggcccag gccaaatggg aactgagcct
     3601 aggcccagcc ggctcggccg ctcgcaaggg gctgcccaaa gagatccaga cgattctcga
     3661 caccgccgcc gaccaacgga cgacgcccca gaccgagacc ctgcaacggc acttccggtc
     3721 gaacgcaccc gagctgaaac cggtccgcga caagatcgcc gcgctcgaga aatcgaaacc
     3781 gattatcccg tcggtgccgg tgatggtcga attgccggcc gatcatcgtc gtgtgactcg
     3841 cattctcaaa aagggtaact ttctcgaccc gggtgacgag gtcaaaccag gggttcctca
     3901 gtcattgcat cgtcttgccg ccgacgctcc caaaaatcgg ctcgggctgg cgcggtggct
     3961 cgtcgattcg gcgaaccctc tgacagcgcg tgtggccgtg aatcgctttt gggctcagat
     4021 cttcgccgcg ggactcgtcg aaaccgaaga agatttcggc acgcagggtg agcc
//
LOCUS       DATAQL010001132         3950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2252628, whole genome shotgun sequence.
ACCESSION   DATAQL010001132 DATAQL010000000
VERSION     DATAQL010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252628"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VHS97_19565"
     CDS             <1..732
                     /locus_tag="VHS97_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450463.1"
                     /translation="SFRPNLVDCVLEERVAPAIANLATVILTTSGYTLIEGFPGANTS
                     GAGSLGASSTGSSSASSVSGAAIPTTLFITGQNGISSLRPGNITGVPSLGAGATGSGG
                     VSISIQVGSGADTADGSTNSVSNNVVGRATVADPTANPTITVIGGSASSDSSSPILPP
                     GQSYRETSAQPTGASTTTPQPTMNGTANTGSPGNTGSMGGNPYAPNPQMGAPTLGPSS
                     MNRGGMVNPIPGSLVPVTPMLPGNN"
     gene            complement(782..1750)
                     /locus_tag="VHS97_19570"
     CDS             complement(782..1750)
                     /locus_tag="VHS97_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX3450464.1"
                     /translation="MNSHRFRRVFAFIFSAIALATVYPSTRAQLFRHTLAQPATIEIL
                     DVGQGDSILIRSPEGKTALIDAGPTKDAALKLLHRKGIKSLDLVALSHHHSDHYGGME
                     EVVRALKPHYFLASKSGHSTSLYLKLLKTVEADGLAVIQPTAHPRKIELGSLELTMFP
                     QPPEDRKEENNNSIGLRLKYGSFAVLFTGDSEESERQWWLRNNPELVRDCTILKLPHH
                     GSRNGTDSRWLQVVRPELAVASLGKNNDYGHPHSETISLLRRSGIPLMRTDQLGTITI
                     TSDGRGWQVIEPRLAHRDHPTQSDIDRVATALDDIPSTRTSQTRKR"
     gene            complement(1747..1992)
                     /locus_tag="VHS97_19575"
     CDS             complement(1747..1992)
                     /locus_tag="VHS97_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX3450465.1"
                     /translation="MSIRLSLDRFEGDNKEIAVLVNDEGEIVNLPKSFLPPGTKPGDV
                     LTLTLERDAVASRRVAKETRRVQDKLSERDPGGDIKL"
     gene            complement(2021..2752)
                     /locus_tag="VHS97_19580"
     CDS             complement(2021..2752)
                     /locus_tag="VHS97_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450466.1"
                     /translation="MSRARKKRGPSLFRRLTYLLVIISGGGAGLGSWVFQDDPKLLAL
                     WTLVTGKPADVDKTQLDGSLVTEVVDALKPRDAFHQPGVYQVTIAQVQLDQKLFKPGH
                     TVNIQARVKRLDQGGRDMTLWDAKTYGERLAVVGKDDLTAGWPHRPFQVEWNPGDQLV
                     LEVYDAKTGLFAPPTRFTLAQTNSSANEFPLKSGDFPLEPVTKPDSPIDPRANHVVLK
                     SEHIGNLGSGDPNPTQVAERPIVIK"
     gene            3175..>3950
                     /locus_tag="VHS97_19585"
     CDS             3175..>3950
                     /locus_tag="VHS97_19585"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3450467.1"
                     /translation="MSATFPLDHITPSPESDRARVVLRRIGALDVLLLSAWCGLAAGL
                     LEAGVRVVCGTIAPFQRLYMVSRHFLWLGPLSSLLLFLGMGLVLSVASKLSPRAVGWL
                     GPRLICACATLPVLMAAFPQVHAEAWAIFGLGIAVQLVPTLERRITALRRRLLWSFPL
                     MLGLVILLASYVFWGDRLKQAREVGRPLPPANSPNVLLIVLDTVRADHLSLYGYERST
                     TPVLERLAKGGIRFDQARATAPWTLPSHASMFTGRWPHEVG"
BASE COUNT          705 a         1165 c         1215 g          865 t
ORIGIN      
        1 agtttccggc ccaatttggt tgattgcgtc ctcgaagaga gagtggcgcc ggccatcgcg
       61 aatctagcca ctgtgatttt gacgaccagt ggctacacac ttatcgaagg gtttcccggt
      121 gcgaacacct ctggcgcagg ctccctgggt gccagcagca cgggaagcag tagtgccagc
      181 tccgtcagtg gtgcggcaat accgaccacc ctctttatca cgggccagaa tggcatctcc
      241 agcctcagac ctggcaatat tacgggcgtt cccagcctgg gagccggtgc aactggctcg
      301 ggaggtgtca gcatatccat ccaggtgggc tccggcgcgg acactgcgga cggatcgact
      361 aacagtgttt ccaacaatgt tgtcggacgc gccacggttg ctgatcccac cgccaatccg
      421 acgataacgg tgatcggagg tagcgcctca agtgactcga gctcacctat cctgccgcct
      481 ggacagagct accgcgagac ctcagctcaa cccactggcg catcgacaac cactccccaa
      541 ccgacaatga acggcacagc caacacaggc agccccggta acaccggcag catgggtggc
      601 aatccatatg ctcccaaccc acagatggga gcgccgactc ttggaccctc gagtatgaac
      661 cgcgggggaa tggttaaccc cataccaggt tcgctcgtgc cggtcacgcc gatgcttccc
      721 ggcaacaact aaagcggttt cggcgctcct ggccaccgcg catcgttcgt ccggacgaat
      781 ctcaccgctt gcgggtctga gaggtccgtg tcgagggtat gtcgtcgagt gcagttgcga
      841 cccggtcgat atccgactga gttggatgat cacggtgcgc caatcttggc tcaatgacct
      901 gccagccgcg cccgtcactc gtgatcgtga tcgttccgag ttgatcggtg cgcatgagcg
      961 gaatacccga gcgccgcagc aggctgatcg tttcggaatg tgggtgacca tagtcgttgt
     1021 ttttgcccag gctggccacg gccaactcgg gccggaccac ttgaagccaa cgcgagtcag
     1081 ttccgttgcg gctgccgtga tgcggaagct tgaggatcgt gcaatcccgc accaactcgg
     1141 gattgttcct gagccaccac tgccgctcgc tttcttcgct gtcgcccgtg aacagcaccg
     1201 caaagctgcc atacttcagc cgcaggccga tggagttatt gttctcctct tttcggtctt
     1261 cgggaggctg aggaaacatg gttaattcga gtgagcctag ctcgattttg cgcggatgcg
     1321 ccgtaggctg gatgacggcc aaaccgtcgg cctccactgt cttcaatagc ttgaggtaga
     1381 gcgaggtaga atggcccgac ttgctcgcca ggaagtaatg gggcttgagc gcgcgcacga
     1441 cctcctccat gccgccgtag tgatcgctgt ggtgatgact gagcgcgacc agatctaacg
     1501 acttgatccc tttgcgatgc agcaacttga gcgccgcatc cttggtcggt cctgcgtcaa
     1561 tcagggccgt cttgccctca ggcgagcgga tcaggatcga gtccccctga ccgacgtcga
     1621 gaatctcgat cgtggccggc tgggccagag tgtgcctgaa aagctgggct cgggtcgatg
     1681 gatagaccgt cgcgagggcg atcgctgaga aaatgaacgc gaagacgcgt cggaaacgat
     1741 gactgttcac agcttgatat ctccaccagg gtcgcgttct gacagcttgt cctggacgcg
     1801 acgtgtttcc ttggccaccc ggcgactggc gacggcgtcg cgctcgaggg tgagggtcag
     1861 gacgtctccg ggcttggtcc ccggcggtaa gaacgacttg ggtagattga cgatctcacc
     1921 ttcgtcgttc acgaggacgg cgatctcctt gttgtcgcct tcgaaacggt cgagagagag
     1981 gcgaatagac acttcgcttg ctcctttggc ccgattgggc tcacttgatg acgatcgggc
     2041 gctcggcgac ctgggtcggg tttggatctc ccgatccaag attgccgatg tgctcgctct
     2101 tgagcacgac gtggttcgcg cgcgggtcga tcggcgaatc gggcttagtg acgggttcga
     2161 ggggaaaatc acccgacttg aggggaaact cgtttgccga ggaattggtc tgggcgagtg
     2221 tgaatcttgt gggtggggcg aagaggcccg tcttggcgtc atagacttcg agaacgagtt
     2281 gatctcctgg attccattcg acttgaaacg gccggtgcgg ccatcccgcg gtcaaatcgt
     2341 ccttgcccac gacggccagt cgctctccgt acgttttggc atcccagagc gtcatatctc
     2401 ggcctccttg gtcgagtctc ttcaccctgg cctggatatt gacggtatgc cccggtttga
     2461 agagtttctg gtcgagctgg acttgggcga tagtgacctg gtagacgccc ggttggtgaa
     2521 acgcatcgcg cggcttcaag gcgtcgacga cctctgtgac gagcgagccg tcgagctggg
     2581 tcttgtcgac gtcggcgggc tttccggtga ccaaggtcca cagggccagc agcttgggat
     2641 cgtcctggaa tacccagcta cccaacccgg ccccgcctcc gctgatgatc accaacaagt
     2701 aagtcagccg cctgaagagc gacggccctc gtttcttcct tgccctgctc atgtcccttt
     2761 ttccatcgtt ttgagcgcgg ttgaccaccg gcgcggctca tcgtggccgc gcttgagggg
     2821 tttagccggc gtttgttctc gatcgatcga tgagtgtcgc gcgcgatccg acacgatcgt
     2881 cgctgcggaa ccgccctttg tttccctgaa cgggtgggcg tgaattgagt cgacgtgggg
     2941 aagcgaggcc tttttagcaa agaacgagaa gcggtcaagg cgagttggtt gattcgaatc
     3001 ggcggacgcc ccatcgggag ccggaatccc aattattttt gatgccagcc cgcgaacgtt
     3061 cggccgggac tatgacggtg ctgtcagcgg actcaagaaa tggctgccgc ttgaggcgaa
     3121 ttggcgcttt tcataataga ggctcactga tcgattgcac gaggccataa tcttatgagc
     3181 gcgacctttc ccctggacca catcactcct tcgccggaat cggaccgcgc ccgcgttgtc
     3241 ctgcgtcgaa tcggggcact tgacgtatta ctgctatcgg cttggtgcgg tctggccgcc
     3301 gggctgctgg aggctggagt cagagtcgtt tgcgggacta ttgctccctt tcagcgactc
     3361 tatatggtga gccgacactt cctctggttg ggcccgcttt caagtctcct cttgttcttg
     3421 gggatgggtc tggtcttgtc ggtggccagc aagctgtcgc cgcgtgcagt cggatggctc
     3481 ggccctcggc tgatctgcgc ctgcgccaca ctgccggtgc tcatggcggc gttccctcag
     3541 gttcacgctg aagcttgggc aatcttcggc ctcggaatcg ccgtccagtt ggtcccgact
     3601 ttggagcggc gcataacggc cttgcggcgg aggctcttgt ggagctttcc gctcatgttg
     3661 ggattggtga tcttgttggc cagttacgta ttctggggag atcggcttaa gcaggctcgc
     3721 gaagtcggcc gtcccttgcc gccggccaat tcgcccaacg tgctcttgat agtgctggat
     3781 acggtgcggg cagaccacct tagcctgtac ggctacgaac gctcgacaac gccggttctg
     3841 gagcgactcg ccaagggagg aattcgcttc gaccaagcgc gcgcaaccgc accctggact
     3901 cttccttcgc acgccagcat gttcaccggc cgctggccgc acgaggttgg
//
LOCUS       DATAQL010001133         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2863653, whole genome shotgun sequence.
ACCESSION   DATAQL010001133 DATAQL010000000
VERSION     DATAQL010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2863653"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..2741
                     /locus_tag="VHS97_19590"
     CDS             222..2741
                     /locus_tag="VHS97_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450468.1"
                     /translation="MVARRLAPVSSSLIRAILIAAAVMPGVACVGTGAGQGLVAWKPE
                     PDQKVQLPAAEQMIDELDRIMTAYGTISVKIPDVWGQDRLARFRCEYESQMAAWLKAG
                     FTGDINASIRRSEAEAARVQVGANLIQPQFNGANTAAQTSVVTLDTATKAQAGMDATL
                     PAVSSPPAKAPASLESTVVLDEHSNYLNHLNQLRRVNLGDDLADRPGYGLYLIRIPVT
                     LSPGPKSRRGKGAIITVSAKPLMTKTTLRSALRNALVNETVNNVTQAICDEWTRDGDR
                     TRGPGTAPYSLVAYADTELFYGHENVQLLRDEAQYQLANDLGDEPHHRVARVSEWIRG
                     ELEATYHLLERAATPVRSPQLASVVDPLEEFGDTVATRDFARIAQMEQAQGADPLVEK
                     AGGQVMVPENDQARRRKRVVSFLSLALKIQAAGINRRLKDDMAAQDPTLVAADLKRIS
                     LFEPEVSDQAFHAFEHYATAKWPLRVYAIEPVIAQQNVADVLGRRTQSALDLVGSGLI
                     GPARGISGFASEHSSADEETAIRLNPTMVGFGAGQSTFGWIFYPRIQTSGARSGRFMT
                     DLVLLLNGRFPDPLGNAQSIEPGQRECTVLIEMPNFVPKIEFTTVADWFRTGEVGDGQ
                     KSDLERASVLGRKLVAAENALNRAQVEGQYRPDEYQIAADRLKQLRDLMPTQRLVVRV
                     PNSDDTNDSRIFCSQGLQLRPSLLSWHGKPPTRGEESTLFLEGRNFSLHDTHVITGGK
                     LATSVLMSRNLIQVTIPKDALSTANAAGNPLIDINVATPNGVSNHLLIPMIAPEPSAK
                     RDPVDKVDQLASHANRDASKGSNCEKPAQAILENHPPIAKQ"
BASE COUNT          601 a          935 c          835 g          518 t
ORIGIN      
        1 cctggccgtc ctaggccgat tgcgggcgaa tcgatccaac ctgataattc acgacgcttt
       61 acgaacgatc gtctcaaacg gattgtccgg tggagcgaaa tcctgtcatt cgaggccgtt
      121 gcctcgcttc cccggcttgg cgagaggccc ggcctttctt acctatgttg ccgccaattc
      181 tggcgacgct cggggccggc tggcccggac ataaggattt catggtcgct cgacgcctgg
      241 ctcccgtttc ctcctccttg attcgcgcga ttctgatcgc ggcggccgtg atgccaggtg
      301 tcgcctgcgt cggaacgggt gccggccaag gcctcgtcgc ctggaaacca gaacccgacc
      361 agaaagttca gcttcccgca gccgagcaaa tgatcgacga actcgaccgc atcatgacgg
      421 cttacggaac catcagcgtc aagataccgg acgtctgggg ccaagatcgg ctcgccaggt
      481 tccgctgcga gtacgagtcg caaatggccg cgtggctcaa ggccggcttc acgggagaca
      541 tcaatgcttc tatccgccgc agcgaagccg aggcagcccg cgtgcaagtc ggtgcgaacc
      601 tgattcagcc ccaattcaat ggcgctaaca cggccgcaca aacgagcgtt gtcactctcg
      661 atacggcaac gaaggcgcaa gcgggaatgg acgcaacgct tccggccgtc agctcccccc
      721 cggctaaggc gcccgcatcg ctcgaatcca ccgtggtact cgatgagcac tctaattacc
      781 tgaatcactt gaaccagttg cgccgggtca atctgggaga cgacctagca gacaggccgg
      841 gctatgggct ctacctgatc cggatcccgg tgaccctatc gcccggaccc aagagccgca
      901 ggggcaaagg agcgatcatc acggtgtcgg caaagcccct gatgaccaaa accacgctgc
      961 gcagcgctct gagaaatgcc ctcgtcaacg agaccgtcaa caatgtgaca caggccattt
     1021 gcgacgagtg gacgcgagac ggcgaccgga ctcggggacc gggcacggcc ccgtattccc
     1081 tcgtcgcgta cgccgacacc gagctgttct acgggcacga aaacgttcag ctcctgcgcg
     1141 acgaggctca gtatcagctc gccaacgatc tgggagacga gcctcatcac cgggtcgcgc
     1201 gggtatcgga atggatccgc ggtgagctcg aggccaccta tcacctcctc gagcgcgccg
     1261 caacgcccgt ccgatccccg cagctcgctt ccgtcgtcga tccgctcgaa gagtttggtg
     1321 ataccgtggc cacgcgtgac tttgctcgga tcgctcagat ggagcaagca caaggggccg
     1381 acccgctggt cgaaaaagcc gggggacaag tcatggtccc ggaaaacgac caagctcgtc
     1441 ggcgcaagcg ggtggtgagc tttctgtccc tcgccctgaa gattcaggcc gcgggtatca
     1501 accgccgtct caaagacgac atggccgcgc aagacccgac cctggtcgcc gccgacttga
     1561 agcggatcag cctgtttgag ccggaagtct ccgaccaagc attccacgcc ttcgagcact
     1621 acgcgaccgc caagtggcct ttgcgagtgt acgcaatcga gccagtcatc gcgcagcaaa
     1681 acgtcgcgga tgtgctggga aggcgcacgc aatcggcgct cgatctggtc ggatccgggt
     1741 tgatcggtcc ggcccgtgga atctccggct tcgcctccga acatagctcc gccgatgaag
     1801 agactgcgat tcgcttgaac ccgacgatgg tcggttttgg cgccgggcag agcacgttcg
     1861 gctggatctt ttacccccgg atacaaacca gcggcgccag aagcggtcga ttcatgaccg
     1921 atctcgtctt gctcttgaac ggacggtttc ccgacccctt gggaaatgcc cagagcatcg
     1981 aacccggcca gcgggaatgc accgtattga tcgaaatgcc gaattttgtt ccgaagatcg
     2041 aattcactac cgtggccgat tggtttcgca ccggcgaggt tggcgacgga caaaagtctg
     2101 acctggagcg ggcttcggtg cttggtcgta agctcgttgc tgcggaaaac gccctcaacc
     2161 gggcccaggt cgaggggcaa taccgacccg acgagtatca gatcgccgcg gaccggctca
     2221 agcagctcag ggatctcatg cccacccagc gcctggtcgt ccgcgtcccc aacagcgacg
     2281 acaccaatga ttcgcggatc ttctgttcgc aaggtttgca gcttcggccg tcacttctaa
     2341 gctggcacgg caagcccccc acgaggggcg aagaatcaac actcttcctc gaggggagaa
     2401 acttcagctt gcacgacacc catgtgatta ccggcggcaa gctggccact tccgtcttga
     2461 tgagccgcaa cctgatccag gtcacgatcc ccaaggacgc cctgtcgacg gcaaacgccg
     2521 cgggcaatcc cttgatcgac atcaacgtcg cgacgcccaa cggcgtctcg aaccacctct
     2581 tgatcccgat gatcgccccc gaaccctctg ccaagcgcga ccccgttgac aaggtcgacc
     2641 agctggcgtc acatgcgaat cgcgatgcgt ccaagggctc aaactgtgaa aaaccagccc
     2701 aggcgattct cgagaatcat ccgccaattg cgaagcagta aatcgagcga cattcgatgg
     2761 cttggccagc gaccatcgcc ggctcaccgc gcatgatcgg ggcatccaat cgcggcgtgt
     2821 actaattttg gcgattttcc gggaattcgg gatgcgtccc ggatcgtcgt cccagtacat
     2881 cacggaaga
//
LOCUS       DATAQL010001134         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5154223, whole genome shotgun sequence.
ACCESSION   DATAQL010001134 DATAQL010000000
VERSION     DATAQL010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5154223"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VHS97_19595"
     CDS             complement(<1..751)
                     /locus_tag="VHS97_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450469.1"
                     /translation="MEGGNLGGRHAEASKWLERATWAFVALGLLVRVVRYLVVYPIWH
                     DEAFLAVNFLDRGYRDLLRPLDYAQVSPILFLWIELTVVRLLGFSEWSLRLFPALCGL
                     ASVILFRHLAVRLLRGIPLVLAVGIFATAFYPVRHSAEIKPYASDLLASLILLTLAVG
                     WWRVPTRSRAWWALAAVVPILLAVSYPAVFVAAGVSVALAPTVFRLGVRRVRLAFLVY
                     NVILIASFLSIYFAATVFQSSALREGYRWGYW"
     gene            1050..3059
                     /locus_tag="VHS97_19600"
     CDS             1050..3059
                     /locus_tag="VHS97_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3450470.1"
                     /translation="MIFSPASIRMMTASLLVGLAFAMAPGAAVAAAPGDSQPDLPIDR
                     GLMQRLSNFTLEDVKAKRPIMLYGYQGRAAIVLVFLGNDCPVGNLYVPRLIELEREFR
                     KKGVVFIGINSNAHESAQDVAKFVADRGIEFPVVKDPENKVADGALIERTCEVIVLDG
                     FARIRYRGAIDDQYQQGKGKDTPDHTYLRDALTAVVTGQVLRVPATKVAGCLLDRVPL
                     KPVQEVKAPRIRPAAPEVVTALAARDKEHPVEVGKVAYSDGVAEILENKCQNCHRPGQ
                     VGPFSLLSYDDARKHSAMIREVIDDRRMPPWHADPRYGHFVNDRSLTTKERATLLAWV
                     DQGAPLGDPTKLPAARQFPEGWTIGKPDVVFELPETYYVPAQGVVSYVYFRVPTNFKE
                     DMWVQAAEAVPGDRSVVHHIIVYVNDSKAARGPGGRPRPMHFTGYAPGDAPSVYPEGT
                     AKKIPAGADLLFQVHYTPIGRVRTDRSKLGLVFSKTKPTREAFTIGIANPDLLLPAKT
                     DNIAVASSMVLSGNARLLSLFPHMHLRGKDFKFTITKPQESPEVVLSVPAYDFGWQTY
                     YVLSEPMDLPKGTRVDCLAHFDNSEGNPYNPDPNKMVRWGEQTFEEMMIGYLDMDVPI
                     GEPVLRGPDFQPTAEKATMATFQALRRIVGGDQVKATGSLRGAPR"
     gene            3075..>3356
                     /locus_tag="VHS97_19605"
     CDS             3075..>3356
                     /locus_tag="VHS97_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450471.1"
                     /translation="MYRSPRYGLTAIVLVTVAGLGFVRISRGGEAESREIPAAFAPLE
                     YLVGRWNGHGVPKDNPAKQFRGWDEKHTWAWMFAQGKPVGLSLTIAGGKV"
BASE COUNT          695 a         1027 c         1014 g          620 t
ORIGIN      
        1 gccagtagcc ccagcgatat ccttcgcgga gcgcgctgct ttgaaagacc gtcgccgcga
       61 aatatatgct caggaatgac gcaatcagaa ttacgttata aacgaggaac gcaagccgaa
      121 cgcgtcgtac gccgagccga aacaccgttg gcgccagagc gacagagacg ccggcggcga
      181 caaaaaccgc cggatacgaa acggccaaga ggatgggaac cactgcggcg agagcccacc
      241 acgcgcggct gcgtgtgggt acgcgccacc aacccacggc cagcgtcaaa agaatcaacg
      301 acgccagcag gtctgacgcg tacggcttga tctccgcgga gtggcggacc ggataaaacg
      361 ccgtggcaaa gattccgacg gccagaacga gcgggattcc ccggagcagc cgcacggcca
      421 ggtgccggaa cagaatcacg ctggccagcc cgcataacgc gggaaacaat cgcagagacc
      481 attccgaaaa tccaagcaga cggacgaccg tgagctcgat ccagaggaag aggatggggc
      541 tgacctgcgc gtagtcgagc ggtcgaagca ggtcgcgata gccgcgatcg aggaagttga
      601 ccgccaggaa tgcctcgtcg tgccagatcg gataaacgac cagatagcgc accacacgaa
      661 ccagtaggcc caacgccaca aaggcccacg tcgcccgttc gagccattta ctggcttcgg
      721 cgtgccgccc gccaaggtta cctccttcca tccccgcacc cgccccttgc caccaatcga
      781 ttccctggcg ccgatgccct ttgcatgtcg tctataatcg acaatttcgc gtccaaagtt
      841 tcacgcagtg atcatccatg actggtcgtg tattttcttg ttttgaaact cggcgtcgat
      901 taggataatg gtgttaacct cgaactcccg gcgttattga ataacgtgaa ctcgtgcgtc
      961 tcgggctcaa tcggcacggc ctcggcggaa acggtccgac cgacgttttg gtcaaccgcc
     1021 gtgaagccga ctctacgtta ggagctggta tgatcttttc tccggcctcc atcagaatga
     1081 tgacggcgtc gctacttgtc gggctcgctt tcgcgatggc accaggcgcg gcggttgcgg
     1141 ccgcgccggg cgactcgcag ccagatctgc cgatcgatcg tggactcatg cagcggctgt
     1201 caaacttcac gctcgaagac gtgaaagcca agcggccgat catgctctac ggttatcaag
     1261 gcagggcggc gatcgtactc gtctttctgg ggaacgactg cccggtgggc aacctgtacg
     1321 tgccccgctt gatcgagctc gagcgcgagt ttcgcaagaa gggcgttgtc tttatcggca
     1381 tcaactccaa tgcccacgag agcgcccaag atgtcgccaa gttcgtagcc gatcgtggaa
     1441 tcgagtttcc cgtcgtgaaa gaccctgaaa acaaggtcgc cgacggcgcc ttgatcgagc
     1501 gcacctgtga ggtgatcgtg ctcgacggct tcgcgcggat ccgctaccgc ggtgccatcg
     1561 acgatcagta tcagcaaggc aaggggaaag acacccctga tcacacctat cttcgtgatg
     1621 cgttgacggc ggtcgtcacc ggccaagtct tgagggtacc ggcgaccaag gtcgccggct
     1681 gcttgctcga tcgcgtgcct ttgaagcccg tgcaagaagt gaaggcaccc cgtattcgac
     1741 cggctgcgcc cgaagtggtc actgcgcttg cggcccgcga caaagagcat cccgtagagg
     1801 tgggcaaggt cgcttactcg gatggcgtcg ccgaaatcct cgagaacaag tgccagaact
     1861 gccatcgacc cggccaggtg ggacctttct cgctcttgag ctacgacgac gcgcgcaagc
     1921 actcggcgat gatccgtgaa gtgatcgacg accgccggat gcctccttgg catgccgatc
     1981 cgcgctacgg tcatttcgtc aacgatcgca gcctgacgac caaagaacgc gccactctcc
     2041 tggcgtgggt cgatcagggc gcacccttgg gtgacccgac gaagctccca gccgcccgcc
     2101 agtttccgga aggttggacg atcggtaagc ccgacgtcgt attcgagctg cccgagacct
     2161 actatgttcc cgcccaggga gtggtctctt acgtctattt ccgggtgccg accaacttca
     2221 aggaagacat gtgggtgcag gctgccgagg ccgtgccggg cgaccggtcg gtcgtccacc
     2281 acattatcgt ttatgtgaat gattcaaagg ccgccagagg tcccggcggg cggccgcgtc
     2341 cgatgcattt caccggttac gccccgggcg acgccccttc cgtttaccct gaaggaaccg
     2401 ccaagaagat cccggcaggt gccgacctct tgttccaggt gcactacacg ccgattggac
     2461 gggtccggac cgaccgctcg aagctgggct tggtcttctc caagaccaag ccgacccgcg
     2521 aggcctttac cattggtatc gccaaccccg acctgttgct gccggcgaaa acagacaaca
     2581 tcgcggtggc gtcgtcgatg gtcctttcgg gtaacgcccg gcttttgagc ttgtttccgc
     2641 acatgcacct gcgcggcaag gacttcaagt tcacgatcac caagccgcaa gaatcgcccg
     2701 aggtcgtgct ttcggtcccg gcctacgatt tcggctggca gacgtactat gttctctccg
     2761 agccgatgga cttgcccaag gggacgcggg tcgattgcct cgctcatttc gacaattctg
     2821 aaggcaaccc ctacaatcca gacccgaaca agatggtccg ctggggtgag cagacctttg
     2881 aggagatgat gatcggctac ctcgatatgg acgtgccgat cggcgagccc gtgttgcgcg
     2941 ggcctgattt tcaaccaacg gccgagaagg cgaccatggc gacgtttcag gccctgcgcc
     3001 ggatcgtggg tggcgaccag gtcaaggcga cgggttcgct tcgaggtgcc ccacgctaaa
     3061 agaggcttgc gaccatgtat cgatctccca ggtatgggct gacggccatc gtcttggtga
     3121 cggtggccgg tttgggcttc gtcaggatca gcaggggggg tgaagcggaa tcacgcgaga
     3181 tccccgctgc gtttgcgccg ctggaatatc ttgtcggccg ctggaacggc catggagtgc
     3241 ccaaggacaa tcctgccaag caattccgag gctgggacga gaagcacacc tgggcctgga
     3301 tgttcgccca gggaaagcct gtgggcttga gcctgacgat cgcgggaggc aaggtt
//
LOCUS       DATAQL010001135         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1796151, whole genome shotgun sequence.
ACCESSION   DATAQL010001135 DATAQL010000000
VERSION     DATAQL010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796151"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..971)
                     /locus_tag="VHS97_19610"
     CDS             complement(51..971)
                     /locus_tag="VHS97_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450472.1"
                     /translation="MVIAAGPGGGYDIYARILATHLTRHISGNPSIIDQNMPTAAGMQ
                     ATNWAFTAAPRDGTVILATFNSLLMEPLYGNAAALYDPLKFEYIGSISKQQNVCTTWH
                     TSPIKTIANAKDREVVVPATGTASDSAILPKVLNAVLGTKFKVILGYSTTDQRLAVER
                     GEAEGVCGLSWSTLKVSAPDWVENHLINVLVQSGSRPQADLPNVPLLVDLVSSPDDKK
                     VIELLSFAQQLGRPFLMPPETPKTMVAVVRRAFDATLKDPLFLADAEKALLEIDPIGG
                     DMMEQMMKGAYATPRPLVKRAAAFAEGSIR"
     gene            1279..>1523
                     /locus_tag="VHS97_19615"
     CDS             1279..>1523
                     /locus_tag="VHS97_19615"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3450473.1"
                     /translation="MTISRANGLDIYYETAGAGPPLVLVHALPFDHNLWLYQIERFSA
                     RFRTVAMDLRGWGRSAKPTTPFSLGDMGNDILGVLAD"
BASE COUNT          299 a          441 c          456 g          327 t
ORIGIN      
        1 taaagaatag ttgctccatc attcacaatg gtgcccatcg gatggtgctt tcaccggatc
       61 gaaccttcag cgaacgctgc ggcccttttg actaggggcc tgggggttgc gtaggccccc
      121 ttcatcatct gctccatcat atctccgccg attgggtcga tctcaagcag agccttctcg
      181 gcatcagcga ggaagagagg atctttgagg gtcgcgtcga atgcgcggcg gacgacggct
      241 accatggtct tcggggtttc aggcggcatc aggaacgggc gaccgagctg ctgcgcgaac
      301 gacaacaact cgatgacctt cttgtcgtcg gggctcgaca cgagatcgac gagcagcggc
      361 acgttaggca agtcggcttg gggcctcgag cctgactgaa ccaagacgtt aatgagatgg
      421 ttctcgaccc agtcaggcgc cgacaccttg agcgtcgacc aagataatcc gcaaacacct
      481 tccgcttcac cgcgttcaac cgcaaggcgc tggtcggtcg tcgaatagcc cagaatgacc
      541 ttgaatttag tgccgagcac cgcattgagc accttcggca gaatcgcaga atcgctggcg
      601 gtaccggtgg ccggtacgac gacctcgcga tctttggcgt tcgcgatggt cttgattggg
      661 ctggtatgcc aagtggtaca gacgttctgc tgcttggaaa tacttccgat gtactcaaac
      721 ttgagcggat cgtagagcgc ggccgcattg ccatacagcg gctccattag aagtgaattg
      781 aaggtggcga gaatgacggt gccgtctctg ggtgccgccg taaacgccca attggtcgcc
      841 tgcatgccgg ctgcggtcgg catattttgg tcgatgatgc ttggattgcc cgaaatgtgt
      901 cgggtcaagt gagttgccaa gatgcgcgca taaatgtcat agccaccgcc tgggccagcg
      961 gcgatgacca tgcgaagctg tttgccgtcg taggcccgat cggtggattg gcccgcagcc
     1021 aggccgagtc caccagccaa cgcaaccgtc aatgttgccg cgaaggccat cgctcgggga
     1081 tagtccgtcc ggcctttggc gtctggcatc gtcgtgctca acattgcgtt ttgctttccg
     1141 atctgccggg tcaaggttcg tattcagtga ctgcggactc tcaacacttc tcagcgtctg
     1201 catgcctccc cgggttgaat ttcctgttcc cggctctgag acgggagcct acagcatacc
     1261 gcgcaaaagg gaatccaaat gaccataagc cgcgccaatg ggctcgatat ctattatgag
     1321 accgctggag cagggccacc gctcgtgctc gtccacgcgc tgccgttcga ccacaacctc
     1381 tggctctatc aaatcgagcg gttctccgca cgcttccgca cggtggcgat ggacctccgc
     1441 gggtgggggc gttcggcgaa gccgacgacg cctttctcgt tgggcgacat gggaaacgac
     1501 attctcggcg tgctggccga cga
//
LOCUS       DATAQL010001136         5794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4084904, whole genome shotgun sequence.
ACCESSION   DATAQL010001136 DATAQL010000000
VERSION     DATAQL010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4084904"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..1211)
                     /locus_tag="VHS97_19620"
     CDS             complement(372..1211)
                     /locus_tag="VHS97_19620"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3450474.1"
                     /translation="MAARDLTIKYTGFDRISFTEPIRWNNLRVWVMLLPAIRQTPWIV
                     ILCSVGWIVELPRSEADEPALLELVGDRGLQAWRKPTGDWIVAAAALPDPARTNRLTA
                     EPGQGTLINGVTGKTRNLLTSEDFGDVDTHFEFLIPKGSNSGVKFEGLYEIQIIDSFG
                     VAKPDGHHCGGIYPRAELLPKYHYLDEGTPPLLNAARPPGEWQTLDVVFRAPRFDSSG
                     KKIKNARFDNVVLNGQVIHRNVELKTPTGHYWRNKEVARGPILLQGDHGPVAFRNIRV
                     RPI"
     gene            1372..2292
                     /locus_tag="VHS97_19625"
     CDS             1372..2292
                     /locus_tag="VHS97_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3450475.1"
                     /translation="MPGVLTGLIPACHTPFDRDGGLNLAAVQAQASLFRATGLRGVFI
                     AGTTGEFSSLTVDERKALCDRWVDVAGDTLRVAVHVGSNCRADAIELAVHARQAGVAA
                     IAVAPPSFFKPATVNDLVDFCAPIAAEVDPLPFYYYHIPSMTGVRLPMSEFLYDARFR
                     IPNLRGLKFSHDDLVDLQGCVALEKGAYDILFGFDEALLAGLCLGVRGAVGCTYNFAG
                     RHYLRLFRAFEAGDITAARAAQFEATQMVKTLASFGFLAASKAVMSLIGVDCGPVRAP
                     LRNLTPAQLDELVGKLAAFDVFERPMKLSS"
     gene            complement(2383..4224)
                     /locus_tag="VHS97_19630"
     CDS             complement(2383..4224)
                     /locus_tag="VHS97_19630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450476.1"
                     /translation="MRTMTLLLAIGIMVAAPPWSRGQQASTDTWLGKRVVQRFNNFPL
                     REGGQAVLRSGTEIHIYRVQRVQGEKLWLEGEEDGPSGWATADQFVHVDKAFAYFSDR
                     IRTHPDDPFFYKARAALSSDRKEFGGALGDWNKVVELEPEDPSSYFGRGNIRLTRREW
                     DKAAGDFTQTIKLDPEHADAHRARAFAWIAQHSFDQSIADCDEAIRLDPEDARAFVIR
                     GRAWLGKQEFDKAIVDCAAAVRLDRSDPRAYTCRALAWGRKKEYDKAIADFNTAINLD
                     PMDAESYFNRAWAWQQKGDNARAMADYNLGVRLDPDAARPRVEPPTKPAAAVDARKTV
                     EEFLRTLPLEQAGAVAVSNDEAHQQKDDGLIPASFDPMPAPASAGQHLTAQAHGVTPP
                     IRGDADAALSRDTFGIVEPQTALEFVTRGGDWLRIKLYDKAIADCTEAINLGCHDPRA
                     RIFRALAWSEKKEYEKAIADYTEAIKVEPQNAFAYAARATAWSAKQDYGRADADLNKA
                     LLLDPENPLQCNGRAWIWATCPDAKYRDGQQGVKAATKACELTNWQEAGIIDTLAAAF
                     AEMGDFGSAQKWQTKAIELESDGENKEEFMTRLKLYQQKQPYRDIKP"
     gene            complement(4402..>5794)
                     /locus_tag="VHS97_19635"
     CDS             complement(4402..>5794)
                     /locus_tag="VHS97_19635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450477.1"
                     /translation="VDCTPEGPVVWNAIRTRLVRTCFFRGALALGVLWAATETKSAQP
                     TTSSGWVGKSVVPKVRKIRLKDLKNGAEHSGPAAIYHVTETNGQSLLLSTPGVAGWAQ
                     IDQVIPVEQAASYFTARIKANPGESFNYTMRAIVSLYQNADLARSLADLNEAIRLAPR
                     DADTRLTRALVWRAKGDPAKAIDEYSEVIRHDPQVVPAYFGRAAIEGEMGELDKSIAD
                     LGSAIRLEPRLADAYIVRAAAWNHQGSIDKAIADLSTAVALDPRNAAAYHDRGLLWSR
                     KKEFDKAISDYGTAIRLEPGTSTGYCSRGFAHKAQKQYDSAIADYSQAVSLDSRDGDA
                     FCGRGWCWLEKKEYARALEDFGQAIRVDPRDACALDGRAWIWATCPNPTYRDGTKTVE
                     AAIEACELTRWKEAYCLETLAAGYAETGDFASAVKWQTKAIELENDPKEKEEYQARLK
                     LFQENKPYREMKP"
BASE COUNT          997 a         1719 c         1791 g         1287 t
ORIGIN      
        1 atcttttgac gtaaatcgag tccggcgcga cttacgttaa gggccacgac cgcaagtctc
       61 gctcagaaag agtgtcgaag gggccgcgcc attgacgtgt cataggacac gcgcgaaaaa
      121 tgccggcagc tcggatgccg cgcacctggt gtgatggtcg ctgggcccgg ggtcagcgtc
      181 gagtgagttg ggtgccggct tccgaggaag gcgctatcga ctcaggacgt ctggccagag
      241 gggcaaggaa aaacccgaga taatgcggcc gagttgtgcg gaaagtacgg tcttatcgct
      301 tcttcgtatc gctcttttgt cgcttctctc tgtcttcgcc cgtgcgaact ctgcgtgctc
      361 tgcggtgaca ttcatattgg gcggacacgg atgttgcgga acgcaaccgg cccatggtcg
      421 ccttggagca ggatcggccc acgcgcgact tctttattgc gccagtaatg gccggttggc
      481 gttttgagct cgacgttccg gtggatgacc tggccgttca agactacgtt gtcgaagcgg
      541 gcgttcttga tctttttgcc gctggagtca aaacgagggg ctcggaaaac cacgtcgagc
      601 gtctgccact cgccgggggg acgtgccgcg ttcaaaagcg gcggcgtccc ttcgtcaaga
      661 taatgatatt tgggcaagag ctcggctcgc gggtaaatgc ctccgcagtg gtgaccgtcc
      721 ggcttggcga cgccgaaact gtcgatgatc tggatttcgt acaggccctc gaactttacg
      781 ccggagttgg agcccttggg aattagaaac tcgaagtggg tatctacatc gccaaagtct
      841 tcgctggtga gtaagtttcg agtcttccca gtgacgccat tgatgagggt gccctgcccg
      901 ggctcggcgg tcagtctgtt cgtgcgcgca ggatctggta gggcggcggc agcgacgatc
      961 cagtcgcctg tgggcttgcg ccaggcttgg agaccgcggt caccgaccag ctcgagcaga
     1021 gccggctcat ctgcttctga gcgcggcagc tcgacaatcc aaccgaccga gcaaagtatg
     1081 acgatccacg gggtttgacg aatggccggt aataacatga cccataccct caagttgttc
     1141 cagcggatag gctcggtaaa tgagatccga tcgaagcccg tgtatttgat ggtaaggtcc
     1201 cgtgccgcca tgggcaacgg gccctcgtcg atttgctcag gcacggtcgg acaagtaaac
     1261 taggtttctt gcgcctgctg cgcttcgcct cggcttacca cttcgaaccc aaccaccgcg
     1321 actggctcgc gcggaggttg ggccctgccc gctcgagccg gagccccgtc catgcccgga
     1381 gttttgactg gactgatccc ggcctgtcac acaccgtttg accgcgacgg aggtctcaat
     1441 ctggcggccg tccaagcaca ggcgtcactt tttcgggcaa cgggattgcg cggtgtcttc
     1501 atcgccggca cgacaggcga gttttcgtcg ctgacggtgg atgaacgcaa agcgctttgc
     1561 gaccggtggg tggatgtcgc cggcgatacg ctgcgcgtgg ccgttcacgt cggatcaaac
     1621 tgccgggccg acgcgatcga gctcgcggtc cacgcgcgtc aggccggagt cgcggcgatc
     1681 gcggtggcgc cacccagttt tttcaaaccg gcgaccgtga acgacctggt ggatttttgc
     1741 gccccgatcg ccgcagaggt cgatccgctg ccgttttact attatcacat tcccagtatg
     1801 acaggcgtgc gccttccgat gtctgaattt ctttacgacg cccgcttccg cattcctaat
     1861 ctgcgcggac tgaagttctc gcacgacgac ctcgtggacc ttcaaggctg tgtcgccctg
     1921 gagaaagggg cttacgacat tctcttcggc ttcgacgaag ctcttctggc aggcctctgt
     1981 ctaggcgtcc ggggggcggt cggctgcacc tacaacttcg ctggacggca ctatctcagg
     2041 ctgttccgcg cgttcgaagc gggggacatc actgcggcac gcgcagcgca gtttgaggcc
     2101 acgcagatgg tcaagacgct ggcgtcattc ggcttcttgg ccgcctcaaa ggcggtcatg
     2161 tcattgatcg gcgtcgactg tgggccggtc cgggctcccc ttcgaaatct cacgccggcc
     2221 cagctcgacg aactggtggg caagctggcg gcctttgatg tgtttgagcg gccaatgaag
     2281 ctaagcagct aaatcgtagg tggggctttc cagcccgacg tcagcctgga acgctgacct
     2341 acgcgcctga cgtcagccgg gaatggctga cccacttcga gctcacggct tgatgtcgcg
     2401 gtacggctgc ttctgctggt agagcttgag tcgcgtcatg aattcttctt tattctcgcc
     2461 atcactctcg agttcgatgg ccttggtctg ccacttctgg gccgaaccga agtcacccat
     2521 ctcggcgaag gcggcggcga gagtgtcgat gataccggct tcttgccaat tagtgagttc
     2581 acaggctttc gtcgcggctt tgactccttg ttgcccgtcg cggtatttcg catccggaca
     2641 cgtcgcccag atccacgcgc ggccgttaca ctgaagaggg ttttccgggt caagcaagag
     2701 cgccttgttg agatcggcgt cggctcggcc gtaatcctgc ttggcgctcc aggccgttgc
     2761 gcgggcagcg taggcaaaag cgttctgagg ttcgaccttg atcgcttcgg tgtaatctgc
     2821 gatggctttt tcgtattcct ttttttcact ccatgccagg gcccggaaaa tgcgcgccct
     2881 ggggtcatgg cagccgagat tgatggcttc ggtgcaatcc gcaatggcct tgtcgtagag
     2941 cttgatgcgc aaccagtcgc cgccgcgggt gacaaattcg agagccgttt gaggctcgac
     3001 gatgccgaac gtgtcgcgag acaaggccgc atcggcatct ccccgtatcg ggggagtgac
     3061 accatgtgcc tgggctgtca ggtgttgacc agcgctcgct ggtgccggca tcgggtcgaa
     3121 tgaggccggg ataagtccat catccttctg ttggtgagcc tcgtcgttgg aaacggcgac
     3181 agcgccggcc tgttccagtg gaagagtacg caggaattct tcgacggttt ttcttgcgtc
     3241 gacggccgcg gccggcttcg tcggtggctc gacccgcggt cttgctgcgt ccggatcgag
     3301 ccggacaccc agattgtaat cagccatcgc ccgcgcgttg tcccctttct gttgccaggc
     3361 ccaggcgcgg ttgaaataag actcggcgtc catgggatcg aggtttattg ccgtgttgaa
     3421 atcggcgatg gccttgtcat attccttctt acgaccccag gccaaggccc ggcatgtgta
     3481 tgcgcgcggg tccgaacgat cgagccgaac ggccgccgcg caatcgacga tggccttgtc
     3541 aaattcctgt ttgcccagcc acgctcgacc gcggattaca aatgcgcgtg cgtcttcggg
     3601 atcgagcctg atcgcctcgt cgcaatcggc gatactctga tcgaacgaat gctgagcgat
     3661 ccaggcgaag gctcgggcgc ggtgggcgtc ggcatgttca ggatcgagtt taatggtctg
     3721 ggtgaagtcg ccggcggctt tgtcccattc cctgcgggtg agccggatgt tgccgcggcc
     3781 gaagtagctt gatgggtcct ccggctcgag ctcgacgacc ttgttccaat cgcccaaggc
     3841 accgccgaac tccttacggt cgctgctgag cgccgctctt gccttgtaga agaatggatc
     3901 atcgggatgg gtccgaatgc ggtccgaaaa gtaggcgaag gccttgtcaa cgtgaacaaa
     3961 ctggtcggcg gtggcccagc cgctcggtcc gtcttcctca ccctcgagcc agagcttttc
     4021 cccttggacg cgctggacgc ggtagatatg aatctcggta ccgctccgca gcactgcctg
     4081 accgccctcg cggagcggaa agttattaaa gcgctgcacg acccgctttc ccagccaggt
     4141 gtccgtggac gcctgttggc ccctggacca tggcggcgcg gccaccatga tgccgatcgc
     4201 caggagcaga gtcatggttc gcattgcatc cgaaatccga aaactcttcg ttttggcggc
     4261 aatcactggc ccctccctgg gctatgcgac tctttccgac gggcgaacca ctcgcggtcg
     4321 cgctttattc cgaacgactg cggcctgttc aaacgggacc gcaccggccc acgggcctta
     4381 ccccgctcgc ctaccgtcgc gtcagggctt catttcgcgg tagggcttat tttcctggaa
     4441 gagcttgagt cgtgcttgat attcttcctt ttctttcggg tcgttttcga gctcgatggc
     4501 tttggtctgc catttcacgg ccgaagcgaa atcgccggtc tcggcgtagc cagcggcaag
     4561 cgtctcaagg cagtacgctt ctttccatct cgtcagctca caggcttcga tggcggcctc
     4621 gacggttttc gtgccgtcgc gataggtcgg gtttggacat gtcgcccaga tccaggcgcg
     4681 accgtccaag gcacacgcgt ctctgggatc gacgcgaatg gcttgtccga agtcctcaag
     4741 ggctctggcg tattctttct tttcgagcca gcaccaacca cggccgcaaa acgcatcccc
     4801 gtcgcgtgaa tcgagactga ctgcctggga atagtcggcg atggcgctat cgtattgttt
     4861 ctgggctttg tgggcaaagc cgcggctaca ataacccgtt gacgtgcctg gctcgaggcg
     4921 gatggccgtg ccatagtcgc ttatggcttt gtcgaattcc ttttttcggc tccagagcag
     4981 accgcggtcg tgataggcgg cggcgttgcg aggatcgagc gcgaccgcgg tactcaggtc
     5041 ggcgatcgcc ttgtcgatcg agccctgatg attccacgcg gccgcgcgca caatataagc
     5101 gtcggcgagc cggggctcga ggcggatggc cgagccaagg tcggcgatcg acttgtcgag
     5161 ttctcccatt tcgccttcaa tggccgcacg gccgaagtac gccggcacga cttgcgggtc
     5221 atggcgtata acctcgctgt attcgtcgat tgccttggca gggtcccctt tggcccgcca
     5281 aaccaaggcg cgcgtcagcc gggtgtcggc gtcgcgtggt gcgagccgga ttgcctcgtt
     5341 caagtcggct agtgatcgag ccaggtccgc attttgataa agcgacacga tagcccgcat
     5401 cgtgtagttg aacgattcgc cgggattggc cttgatccgc gcggtgaagt acgaagctgc
     5461 ctgttccaca ggaatgacct ggtcgatctg agcccagccg gcgactcccg gcgtcgacaa
     5521 aagcaatgat tggccgttcg tctctgtaac gtggtaaatc gccgccggcc cagagtgctc
     5581 ggcgccattc tttaggtctt tgagacgaat ttttcggacc ttcggcacca cggatttgcc
     5641 gacccagccg cttgaggttg ttggctgggc gctcttggtc tcggttgccg cccagagcac
     5701 tccgagcgcc agtgcgcctc ggaagaaaca ggttctgacc agcctcgttc tgattgcgtt
     5761 ccacacgacc ggtccctccg gagtgcaatc aacc
//
LOCUS       DATAQL010001137         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4237940, whole genome shotgun sequence.
ACCESSION   DATAQL010001137 DATAQL010000000
VERSION     DATAQL010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4237940"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VHS97_19640"
     CDS             <1..1576
                     /locus_tag="VHS97_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX3450478.1"
                     /translation="DFRDVAQESGVVFHFDSGSRGKHDLPEIMGGGMALFDADGDGRL
                     DIYLCNGGPIEPVPGKPDPPWRLFRNLGVWHFDDITDRAAAPGPSYAMGTAVGDYDGD
                     GRVDLFVSGWRDQRLYRNLGRGRFQDVTQSAGLASNLWSTSAAFADLDGDGDLDLYVA
                     NYLDFDPKSPPYCAAPDGRRDYCGPEDFAAQPDRLYRNDGDGTFTDVARLAGIEKPDG
                     RGLGVLIAELTGDNRPDIFVANDGTSCWLFANRGHMQFEEIGETAGVARDGQGRALAG
                     MGVAAGDVDGDGRCDLLVTNFLGRSTIAFGAQADPSRVYRDDTSRFGLTAATRHVLGF
                     GIVLADFDGDGWPDLVQNNGHVLDRARLGTPLMMRPTLLANQNGRFDDLAIQAGGWFD
                     RPTLGRGLAMADLDGDARPDLVACSLDAPAALLRNTTAGRNFVCLNIVDRENRPAVGA
                     RVAVTAGGRRQTGVVAAGGSYLSTTDARLWFGLGPASSIERIEVEWPWGQRELWAPPL
                     RAIHEPLLIKQGKGTP"
     gene            1616..2473
                     /locus_tag="VHS97_19645"
     CDS             1616..2473
                     /locus_tag="VHS97_19645"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX3450479.1"
                     /translation="MPERPDPQGHTLQCMEIWGGIEPVERAVSTPGLDLWVYSQPFEG
                     DAQGGDVYYVTLCGGGIITRLVVADVSGHGSSVAEFSLSLRSLLRKNINQKSQKRLVE
                     RLNRQFTEMAGLRRFATALVVTYLASTNRLSVCNAGHPRPLFYRAATGTWSLLKPHAG
                     GADVGGNLPLGLDLETRYQMFDLTLAPGDRAVFYTDALTEAADESGKLLGETGLLDAA
                     SRLDAAVSSPGEIGTCLLRDIERHRGLRQAGDDVTLIVLHHRASGPRRLSVGQKVDVY
                     AKVFGLKQV"
     gene            2498..>2843
                     /locus_tag="VHS97_19650"
     CDS             2498..>2843
                     /locus_tag="VHS97_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450480.1"
                     /translation="MKRSILFAVVFSWLVTWGIAVADEAVIPRPEHPRPDARRPHWAS
                     LNGRWEFRFDPLDAGVAAGWHKPGAPGYDRTILVPFPWESELSGIHQVKDAPKIGWYR
                     RRFTVPETFPADQ"
BASE COUNT          512 a          870 c          910 g          551 t
ORIGIN      
        1 tgatttccgc gacgtcgccc aagagtccgg tgtcgtgttc cacttcgact ccggctcgcg
       61 tggcaagcac gacctgcctg aaattatggg aggcggcatg gctctcttcg acgccgacgg
      121 agacggccgt ctcgatatct acctctgcaa cggcggaccg atcgaaccgg taccgggcaa
      181 acctgacccg ccctggcgtc tcttccggaa tcttggcgtt tggcactttg acgatatcac
      241 ggatcgagcc gccgctcccg gaccgagcta cgcgatgggg acggctgtcg gcgattacga
      301 tggcgacggc cgagtcgact tgttcgtttc cggctggcgc gatcagagac tctatcgcaa
      361 tctcggaagg ggacgcttcc aggacgtaac ccagtcggcg ggcctggcat caaatctctg
      421 gagcacctcg gcggcattcg ccgatctcga cggcgacggc gacctagacc tctacgtggc
      481 caactatctc gattttgatc cgaagtcacc accttattgt gcagctcccg atggccggcg
      541 tgactattgc gggccggaag actttgcggc ccagccagat cgactgtatc ggaacgacgg
      601 cgatgggacg tttacagacg tggcgagatt agccgggatc gaaaaaccag atgggagagg
      661 cctcggcgtc ctgatcgccg agctgacggg tgacaatcgc cccgatatct tcgtcgccaa
      721 cgacggcaca tcctgctggc tgttcgcgaa ccgcggacac atgcaattcg aggaaatcgg
      781 cgagacggcc ggagtcgcgc gcgacggcca ggggcgtgcc ctcgccggca tgggagtcgc
      841 cgccggcgac gtggatggcg acggccggtg cgaccttctc gtgacaaact ttctcggtcg
      901 ttcgaccatc gcgtttgggg cgcaggcgga cccgtcgaga gtttaccgcg acgacacgag
      961 tcgttttggt cttacggccg ccacgcgcca cgtgcttggt ttcgggatcg ttttggcgga
     1021 tttcgacggc gacggttggc ccgacctcgt ccaaaacaac ggtcatgtgc tggatcgagc
     1081 tcggctgggt actccgctaa tgatgcggcc tacgttgctc gccaaccaga acggcaggtt
     1141 cgacgatctc gcgattcagg ccggcggctg gtttgaccgg ccgacactgg gccgaggact
     1201 ggcgatggct gacctggatg gcgacgcacg gcccgacctg gtggcctgct cgctcgacgc
     1261 gcccgctgcg ctcctgcgta acacgaccgc cggtcgaaac ttcgtgtgcc tgaacatcgt
     1321 cgaccgggaa aaccggcccg cagtgggtgc tcgtgttgcc gtcacggctg gtggtcggcg
     1381 ccagaccggc gtggtggcgg caggggggag ctacttgtct acgacggatg cccgcctttg
     1441 gtttggcctg ggcccggcgt cgtccatcga gcggatcgag gtagagtggc cttggggaca
     1501 gcgcgagctc tgggcgccac cccttcgagc aattcatgaa cccttgctca tcaaacaggg
     1561 gaaaggtaca ccataacgcc aataaaacac gagcgttgaa cggagaccgt cggacatgcc
     1621 ggaacgccct gatcctcaag gacacacatt gcaatgcatg gagatctggg gagggatcga
     1681 gcccgtggag cgcgcggtct caacacctgg tcttgatctc tgggtctaca gtcagccgtt
     1741 tgaaggggac gcgcaagggg gcgatgtcta ttatgtcacg ctctgtgggg gcgggatcat
     1801 cacgcggctc gtcgtcgccg acgtttcggg acacgggtca tcggtcgccg aattctcctt
     1861 gtcgctccga tcgctcttgc gaaagaacat caatcaaaag agtcagaagc ggctggttga
     1921 gcggcttaac cgccagttca ccgagatggc gggccttcgg cgatttgcga ccgcgcttgt
     1981 cgtcacttac ctcgcatcca cgaatcggct gtcggtatgc aacgcgggcc atccccgccc
     2041 gctcttttac cgggcggcca ccggcacctg gtcgctgctc aagccacacg ccggcggcgc
     2101 cgatgttggc ggcaacctac cgctcgggct cgatttagaa acccgctacc agatgtttga
     2161 cctgacgttg gcaccgggcg accgtgcggt cttttacacc gacgctctca ccgaggccgc
     2221 cgatgagtct ggaaagttac tgggggagac aggattactc gacgccgcaa gccggctgga
     2281 cgcggcggtc agtagccctg gagagatcgg gacctgtctg ttacgagaca tcgagcgcca
     2341 tcgcggcctc cgccaagcgg gggatgatgt gacgttgatc gtgcttcacc atcgcgcttc
     2401 gggcccgcgc cgcctgtcgg tcggacagaa ggtcgacgtg tacgccaagg tcttcggttt
     2461 gaaacaagtt tgaacttaaa tcgctggaac caacatcatg aagcgttcga tcttattcgc
     2521 cgttgttttc agttggctcg tgacatgggg aatcgctgtc gccgacgaag ccgtcatccc
     2581 tcggcccgag catccgcgac ccgatgctcg acgtccacac tgggccagct tgaatggccg
     2641 ctgggagttc cggtttgatc ccctggatgc aggggtagca gcgggttggc acaagcccgg
     2701 ggcaccggga tacgaccgca cgatcctggt gcccttccct tgggagagcg agctgtcggg
     2761 cattcaccag gtcaaagatg cgccgaagat cggctggtat cgccgccggt tcactgtccc
     2821 cgagacgttt cccgccgacc agc
//
LOCUS       DATAQL010001138         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3933484, whole genome shotgun sequence.
ACCESSION   DATAQL010001138 DATAQL010000000
VERSION     DATAQL010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933484"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1462
                     /locus_tag="VHS97_19655"
     CDS             287..1462
                     /locus_tag="VHS97_19655"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEX3450481.1"
                     /translation="MDSDRLFGRYRELQSYVGWTAADAERIVAAAALLDPCLHDLVDD
                     FYAEIERHPAARKVITGGQAQIDRLKGTLVQWIRELLAGRYDADYVARRWRVGWRHVE
                     IGLEQVYTNVALSRLRTGLIRALHVSWLHDAQALKETVRALNKLLDLDLAIIEDAYQA
                     EYMARLQRTERLATLGQVAGGVAHELRNPLNVVKTSVYYLLNARNPTPEKKADHLKRI
                     ERNVELADNVITSLSNFAKVPVPALLPIAIEPLLCEALEINPPGEGIVVTVDCPAGLP
                     RALGDSDQLRIALGNLIRNARDAMIQGGKLSISAVANDGFVDIAVADSGVGIEATELV
                     RIMEPLYSTKARGLGLGLAITRSIVEKNQGSLSVSSEPGRGSTFTIRLTALPPDGER"
     gene            1462..2226
                     /locus_tag="VHS97_19660"
     CDS             1462..2226
                     /locus_tag="VHS97_19660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450482.1"
                     /translation="MMRRLEPRILVVDDDPDICDNLRDILCDLGYQVDTAHDGPSALE
                     LVDRQPYDIALLDLKMPGMDGLTLYREIKKRHAGTVSLLVTAFAGASTAEEALSAGAW
                     SVLAKPVDFSKLLHLVEKALDQPLVLVVDDDRDLCENLWDLLRDRGFRVCLAHDLHNA
                     AEKLRDSSFDVVLIDMRIPDGDGGSVFRMVRTSNPESRTVLITGYRADMDQLVAQLLA
                     EGADAVQYKPFHIPDLIETLGKLAAAREDDAGGARR"
     gene            2223..>2581
                     /locus_tag="VHS97_19665"
     CDS             2223..>2581
                     /locus_tag="VHS97_19665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450483.1"
                     /translation="MTVMASSPPVDILVIEDDPDARANLRDILELDFHRVTAVGSATE
                     ALQQANLERFSAIILDRRLPDATAEQLMPKLKAADPNAAVIVVTGYSDLDGAIAALRQ
                     GATDYILKPFNPDFLRT"
BASE COUNT          523 a          785 c          768 g          505 t
ORIGIN      
        1 cgcaagacgg ggccgcctta gccaacattc cctagccctt catgagcaag gataagcgga
       61 tcgtaactca agctatctga agagtcaatc tcatttttcc gagaagtcgg caaattgtgt
      121 cgagaccact tcccatcatt gtgccttgtc ctgtaatata tatgaagaca attgaccgag
      181 ttgctcacga aagggcgatt cggaggggta atgtaggccc acgcaaccgc ccaaaggcga
      241 gcgtgggctt tttttgttgt acgctcgtac tcgagtgaga cacgtcatgg actcagatcg
      301 gttgtttggc cggtatcgcg agctccaatc gtacgtgggc tggaccgccg ccgacgccga
      361 acggatcgtc gcggctgccg cgcttttgga cccgtgtctc cacgatcttg ttgatgactt
      421 ctacgccgag atcgagcggc accccgcagc tcgcaaggtg atcaccggcg ggcaagccca
      481 gatcgaccgg ctcaagggga ctctggtgca gtggatccgc gagctcttgg ccggcaggta
      541 cgacgccgat tacgtagcac gacgatggcg ggtcggttgg cggcatgtcg aaattggcct
      601 cgagcaagtt tataccaacg tcgcactgtc gcggttgcgt acggggctca ttcgcgcgct
      661 ccatgtgagc tggctacatg atgcccaggc tttgaaagag acggtccgcg cgctcaacaa
      721 gcttttggac ctcgacctgg cgatcatcga agatgcctat caggccgaat acatggcccg
      781 gctgcagcgc accgagcgat tggcgacgtt ggggcaggta gccggcggcg tcgcacacga
      841 gctccgcaac ccgcttaatg tggtcaaaac gtcggtttac tacttgctca atgctcgtaa
      901 cccgaccccc gagaaaaagg ccgaccactt gaagcggatc gagcgtaatg tcgagctggc
      961 cgacaacgtg atcacgtcgc tgtccaattt cgccaaagta cccgtgcctg cgcttctgcc
     1021 gattgcaatc gagcccttac tctgcgaagc cctcgagatc aacccgccag gcgaaggcat
     1081 cgtagtcacg gtcgattgtc ccgctggtct gccgcgtgcg ctgggcgaca gtgatcagtt
     1141 gcggattgca ctgggcaact tgatccgcaa cgcgcgcgac gcgatgatcc agggaggaaa
     1201 gctctcgatc tcggccgttg caaacgacgg cttcgtggat atcgcagtgg ccgatagcgg
     1261 cgtcggcatc gaggcaacgg aactggtcag gatcatggag cctctctact cgacgaaagc
     1321 gcggggactc ggtctcggcc ttgcaattac tcggtcgatc gtggaaaaaa accagggtag
     1381 cctgtctgtg agcagcgagc cgggtcgcgg cagtaccttc acgatccgac tgaccgcgtt
     1441 acctcccgac ggagaaagat gatgatgcgc cggttggaac cccggattct tgtcgtggac
     1501 gacgacccgg atatctgtga caacctgcga gacatcctgt gcgacctggg ctatcaggtc
     1561 gacaccgcgc acgacgggcc ctcggcgctc gagctggtgg accgccagcc ctacgacatc
     1621 gcgcttttgg atttgaagat gcccggaatg gatggactga cgctctaccg cgagatcaag
     1681 aaacggcatg ccggcacggt ttcgcttctg gtcacggcct ttgccggcgc ttccacggcc
     1741 gaggaagctc tttcggcagg cgcatggagc gtgctcgcca agccagtcga cttctccaaa
     1801 ctgttacact tggtggagaa agcgcttgat caaccactcg tcctggtcgt cgacgacgat
     1861 cgcgacctgt gcgagaacct ctgggacctg ctacgagacc ggggctttcg cgtctgcctg
     1921 gcccatgacc tccataatgc ggccgaaaaa ctccgagatt cctcattcga cgtggtcctt
     1981 attgacatga gaatcccgga cggcgacgga ggcagcgtct tccgaatggt ccgcacatcc
     2041 aaccctgaat ctcggacagt gctgatcacc ggctatcgcg cggacatgga tcagctcgtc
     2101 gcgcagcttc tcgccgaagg ggctgacgcg gtccagtaca aaccgttcca catcccggat
     2161 ctgatcgaga cgctgggaaa actcgccgcg gcgcgcgaag acgatgcggg aggggcgcgg
     2221 cgatgaccgt catggccagt tcgcctccgg tcgatatcct ggtgatcgag gacgatcccg
     2281 acgcgcgggc caacctgcgt gacatactcg agctcgattt tcaccgggtg acggccgtgg
     2341 ggagtgcgac cgaggccctg cagcaggcca atctggaacg attctccgcc attattctcg
     2401 accgccggct gcctgacgcg acggccgagc agctcatgcc caaactcaag gctgccgatc
     2461 ccaacgccgc agtcatcgtc gtgaccggct actccgacct tgatggagcc attgccgctt
     2521 tgcgccaagg cgcgaccgac tacatcctga aaccgttcaa ccctgacttc ctgcgcacca
     2581 g
//
LOCUS       DATAQL010001139         3944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1640749, whole genome shotgun sequence.
ACCESSION   DATAQL010001139 DATAQL010000000
VERSION     DATAQL010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640749"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VHS97_19670"
     CDS             <1..1153
                     /locus_tag="VHS97_19670"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450484.1"
                     /translation="LERDRDGRFRYALLANRGLVRFESRKLDLAIADLEEAIELDPRQ
                     LSAHVTLAQIHRQQHQLDLALSELSRAIALEPGLAPLYRTRACWNLESPRVTQEVRAQ
                     VIADLERAIRLDAPNSRDLAKDHAEMGRLLLLDKQFQRALEACDRALKIDPDDPEVHR
                     WRVGALLELSRPSEAIEACDAYLRAGRSSADLLGLRGLAKAKRNDFVGAIDDYTLALA
                     KEPRSPVVRGRRGWAYLVSGAPQLALRDFEEVIRNDSSNADAYSGRGSARAALGHCRL
                     AVADAEESLRHGEPEARLLYSAARILALAAESAKAEPSRRVLSDAAAAEGQCERALDL
                     LRQAVERTPPQERAEFWSNVVQSDHAFTAIRRHSAYARIGVAAALPRPR"
     gene            1209..2843
                     /locus_tag="VHS97_19675"
     CDS             1209..2843
                     /locus_tag="VHS97_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450485.1"
                     /translation="MNWRNKPRARPRRSAAESRFQLETLEDRTALSVAWGAPAPAPLL
                     RPPVDVRLDAIRAVPLLNLDSTALAIDQAALPVAAPSAPFDPSNVSSLPITEFAVKGS
                     GVIESPLAGALSMASGGTTIASLVVSGGDVVPKSSALRPALDSMGTFAGSSATEPTDV
                     IGRAIRSVDLMGSFDPRAGDPWFQASPAFGPDDAFGLALWRGFAAANPDENQTEMSLS
                     GPLLWIENDTALLTWMSMTQASGRSAGRGSSQIFADLALQMNPTGEVGGSSGLLYLAT
                     GGWTSLLDEISTMREDEVLLVDRGPTESETTASVLDAGIPLNMLLWGTDAPIPNEQGG
                     VEQVAELIPLSESSLALAATLWTVRSDSQASAPGSELAAGAGADPVISSLSPEYWAVF
                     MTGVDRAFEQTFRDVQVDTFASDGRHRKSEEAQRGLDDRLQWQRPILPGAAEELRGGK
                     QRSTRPGHGSGDNDARTPGRPAVSHRTGDEHGEETSQAPRVAQPQDSEVQPVVLASTP
                     VLWVVSISSIVAGWFWGKKRERRQRSGLGASHSSCR"
     gene            complement(2885..3520)
                     /locus_tag="VHS97_19680"
     CDS             complement(2885..3520)
                     /locus_tag="VHS97_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3450486.1"
                     /translation="MPFATAERHEGDTMSPFSDSQTSLTLMEMLREQPQNALAWDKFV
                     RRYHPKIYGWCKAWGLQEADAEDVAQDVLAKLTQKMASFRYDQSRCFRAWLKTITQRV
                     LSDLIANRKRSLGGQAIPLLETRDARADLERRIEEIFNRELLELAMLRVRARVAQPTW
                     EAFRLMALEECTGAQASQVLGIPIASVFVAKHRVQKMLKEEIRKLDGSVDE"
BASE COUNT          740 a         1258 c         1194 g          752 t
ORIGIN      
        1 gctcgagcgt gatcgagacg gacgattccg ctatgctctc ctggccaatc gcggactcgt
       61 acgattcgag agccgaaagc tcgatctagc tatcgccgat ctggaagagg cgatcgaact
      121 tgatcccagg cagttgagcg ctcatgtcac cctcgctcag atccatcgac aacagcatca
      181 gctcgacttg gccttgagcg agctcagtcg cgcgattgcg ctggaaccag gcctggcccc
      241 tctctatcga acccgcgcgt gctggaacct cgagagtccc cgggtaactc aggaggttcg
      301 tgcccaagtc atcgccgacc ttgagcgggc gatccgcctg gacgcgccca acagccgcga
      361 cctggcgaag gaccacgccg aaatgggccg cttacttctc ctggacaaac agtttcagcg
      421 ggcactcgag gcctgcgatc gagcgctcaa gatcgatccc gacgaccccg aggtccaccg
      481 ctggcgcgtc ggcgctctcc tcgagctctc gcgcccgagc gaggcgatcg aggcgtgcga
      541 cgcctacctg cgcgcgggcc gttcctcggc cgaccttctc gggttgcgcg ggctcgccaa
      601 ggccaagcgc aatgacttcg tcggcgcgat cgatgactat acgttggcac tggctaaaga
      661 gccaagatcg cccgtggtgc gaggccggcg cgggtgggcc tatctcgtgt ccggagcccc
      721 ccaactggca ctacgcgatt tcgaggaagt cattcgcaac gactcctcaa acgcagacgc
      781 ctacagtggg cgtgggtcgg cgcgtgctgc actgggtcac tgccgtctgg cagtcgctga
      841 cgccgaagag tcgctgcgtc acggcgaacc cgaggcccgg ctgctttata gcgccgcccg
      901 catcctggct ctggccgccg aatccgccaa ggccgagcca agccgccgtg ttttgtcgga
      961 cgcagcagca gcagagggcc aatgcgagcg agcgctcgat ttgctccgcc aggcggtcga
     1021 gcggactcct ccccaagagc gcgccgaatt ctggagcaat gtcgttcaat cggatcacgc
     1081 cttcacggcg atccgccgac attcggcata tgcccgaatc ggcgtggcgg cggcattgcc
     1141 gcgaccccgg tgaaattaca atacgccgca ccaagggatt aagcgtgctg atcaatcgga
     1201 ttctcaccat gaactggcgc aacaaaccca gggctcgacc tcgacgttcc gccgccgagt
     1261 cgcgatttca gttggagact ctcgaggacc gcacggcact ctccgtcgcg tggggtgccc
     1321 ccgcgcctgc gccgctgttg cgcccccccg tggacgtacg attggatgcg atccgcgcgg
     1381 tgcccctgct gaatctcgac tcaaccgcgc tcgccatcga ccaggctgcg ttgccggttg
     1441 ccgcgccgag cgctccgttt gatccgtcga atgtgtcttc tttgccaatc accgaatttg
     1501 cggtgaaagg ttcgggcgtc atcgagagtc cgctggccgg cgcgctctct atggcaagtg
     1561 gtggcaccac gatagcctcg ctcgtggtat ctggtggcga tgtcgtccct aagtcgtccg
     1621 cgctacgccc cgcgctagac tcgatgggta cctttgcagg ttcttcggca accgagccga
     1681 ccgacgtgat aggaagggcc ataagatccg tggacctgat gggatctttt gatccgcgag
     1741 ctggagatcc ctggtttcag gcgagtcctg cgtttgggcc ggacgacgcg ttcggacttg
     1801 ccctgtggcg cggctttgca gcggcgaacc cagatgaaaa tcagaccgaa atgagcttat
     1861 cggggccact gctgtggatc gaaaacgaca cggctctctt gacatggatg tccatgacac
     1921 aggcctctgg tcgttccgcc ggtcgggggt cgagccagat cttcgcggac ctggctcttc
     1981 agatgaatcc taccggcgaa gtcggcggct cgagcggttt gctttatctc gcgactggag
     2041 ggtggacgag tttgctcgac gagatatcaa caatgcgcga agacgaggta cttctggtcg
     2101 atcgcgggcc gaccgagtcg gaaaccacag cttcggttct agacgccggt atacccttga
     2161 acatgctcct ctggggcacg gacgcgccga tcccgaacga gcaagggggc gtggagcaag
     2221 tcgccgagct aataccgctt tcggagtctt cgctggcgct cgccgccacg ctgtggaccg
     2281 tacgctcgga ttctcaggcg tctgctcccg ggtcggaact cgcggccggc gccggcgcgg
     2341 atccggtcat ctcgtccctc tcgcccgaat attgggcggt ttttatgacc ggcgttgatc
     2401 gagccttcga gcagactttc cgcgatgtcc aggtcgacac ctttgccagc gacggccggc
     2461 acaggaagag tgaggaagct caaagaggtc tggacgaccg actccagtgg caacggccga
     2521 ttctcccagg tgccgcggag gaactgcgag gcggcaagca acgatcgacc cgacccggtc
     2581 acgggtcggg cgacaacgac gccaggacac cgggacggcc ggccgtctcc caccgtaccg
     2641 gagacgaaca cggagaagaa acttcacaag cccccagggt tgcccaaccg caggacagtg
     2701 aggtgcaacc cgtcgttctg gcgtcaacgc cggtgctttg ggtggtgtcc atttcgtcga
     2761 tagtcgccgg ttggttctgg ggcaaaaaac gcgagcgacg gcaacgctcg gggctgggag
     2821 caagtcattc cagctgtcgt tgaagctgcc ccatcgcgtc agacacccag gcgtccgatc
     2881 ggtctcactc gtcgaccgat ccgtccagct tgcggatctc ttccttgagc atcttttgga
     2941 cgcgatgctt ggccacgaaa accgacgcga tcggtatgcc cagcacctgc gatgcttgag
     3001 cgccggtgca ctcttcaaga gccatcaatc ggaaggcttc ccaggttggt tgtgctacac
     3061 gcgcgcggac tcgcaacatt gcgagctcga gtagctcgcg attgaagatc tcctcgattc
     3121 gcctctccag atcggcccga gcgtcgcgag tctccagcaa cggaatcgcc tgcccgccga
     3181 gcgaccgctt gcgattggcg atcagatcgc tcaggacgcg ttgggttatc gtcttcaacc
     3241 aggcacggaa acagcgcgac tggtcgtagc ggaagctcgc cattttctgg gtcagctttg
     3301 caagcacatc ctgggcgacg tcttccgcgt ctgcctcttg aagaccccaa gccttgcacc
     3361 acccgtaaat cttggggtgg tatcgccgca cgaatttgtc ccacgcgagc gcgttctgcg
     3421 gttgttccct caacatctcc atcagtgtga gcgaagtctg ggagtcgctg aacggcgaca
     3481 tcgtgtcccc ttcgtggcgt tccgcggtgg cgaacggcaa aaacccattc ttgtgtcgaa
     3541 aaatggacca caaagccgac gtgcggtcac gcaattctac tggttgcgcc gaatcctgca
     3601 cccttttttt ccgccactgt gtaagtttgc ggcggctcgg aaagtatttt ctgcatcttg
     3661 gagcggcggc aaagcccaac acgcatcgag tgaccgtgca cgcacccaag aatcaacgga
     3721 aaaccggaca cggctcgcca atccggcacc gcgcggctag ttgccgctcg gagttccgcc
     3781 acccacgccg aagggttgcg acaacacaag ccggattagg cgagtcgtgt cctctccaaa
     3841 aaaacgcacg ctggttccgc gaccagagtt cacttgtcaa atcgagtcaa gatgccctcg
     3901 atggcatcgg catgaatttg ccacgacagg gtggaatacc cccc
//
LOCUS       DATAQL010001140         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2253117, whole genome shotgun sequence.
ACCESSION   DATAQL010001140 DATAQL010000000
VERSION     DATAQL010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253117"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VHS97_19685"
     CDS             complement(<1..252)
                     /locus_tag="VHS97_19685"
                     /inference="COORDINATES: protein motif:HMM:NF023970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450487.1"
                     /translation="MAQSPEIDDDALLALKPNRLVSIRELFGVDSDMLVPAFTERDSH
                     VPDLDTAYRFDPQTTLAICAGLAHDRRVMIQGYHGTGKST"
     gene            complement(306..935)
                     /locus_tag="VHS97_19690"
     CDS             complement(306..935)
                     /locus_tag="VHS97_19690"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX3450488.1"
                     /translation="MAVRFQYKPRFVDIRVRPPEENGDAREDVTALKPGERPCEHPGC
                     HAAGSTRAPKSRDLLGEHYWFCQAHAGEYNRSWNFFAGMSEDEVQARISDELVSGGRP
                     TWPLKASPKSRESAAARGFFRDPFNLFAGSAQARERAAKERTIGRLEMQALADLNLEP
                     GAERPAIRARYTELVKRCHPDANGGDRSAEHKLQRVIRAYKTLRKAKLA"
     gene            1002..1265
                     /locus_tag="VHS97_19695"
     CDS             1002..1265
                     /locus_tag="VHS97_19695"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX3450489.1"
                     /translation="MGAVARSMEAKLEASLAPSRLELTDESARHQGHAHAGVESHFNL
                     TVESAAFTGLGRLARQRLVMRVLADELAGPVHALSIRALAPGE"
BASE COUNT          243 a          483 c          544 g          278 t
ORIGIN      
        1 ggtcgatttg ccggtgccgt gatagccctg gatcatcacc cggcggtcgt gcgccaggcc
       61 ggcgcagatg gcgagtgtgg tctgcggatc gaagcgatag gcggtgtcca agtccgggac
      121 gtggctgtcg cgctcggtaa aggccggaac cagcatgtcg gaatccacgc cgaacagctc
      181 gcggatggag accagccggt tgggcttaag cgccagcagg gcgtcgtcgt cgatttccgg
      241 ggattgggcc attaccgctg ttcactagaa taagcttttg gcggacgcaa acacgccctc
      301 cttcgtcagg cgagcttggc cttacgcaag gtcttgtagg cccggatcac ccgttgcagc
      361 ttgtgctcgg ccgatcggtc gccaccgttg gcgtccgggt ggcagcgctt gaccagttcg
      421 gtgtaacgcg cccggatggc cgggcgttcg gccccgggct ccagattgag atcggcgagg
      481 gcctgcatct ccagacggcc gatggtccgt tctttggccg cgcgctcgcg ggcctgggcg
      541 gagccggcga acagattgaa cggatcgcga aagaagcccc gggcggcggc cgactcgcgg
      601 ctcttggggc tggccttgag cggccaggtc gggcgcccgc cggagaccag ttcgtcgctg
      661 atccgggcct ggacttcgtc ctcgctcatg ccggcgaaga agttccacga gcggttgtat
      721 tcgccggcgt gggcctggca gaaccagtag tgctcgccca ggagatcgcg ggacttcggc
      781 gcccgggtcg agccggcggc atgacacccg ggatgctcgc acggccgttc gcccggcttc
      841 aaggcggtga cgtcctcgcg cgcgtcgccg ttctcctccg gcgggcgcac gcggatatcg
      901 acgaagcgcg gcttgtattg aaagcggacg gccatgggct gaacttaagg gcgcgataag
      961 ctacagttca agaattgact ttgcatgatt aggggtccga aatgggcgcc gtggcccggt
     1021 ccatggaggc gaaactcgaa gcttccctgg cgccgtcccg cctggagctg accgatgagt
     1081 cggcccgcca ccaggggcat gcccacgccg gcgtcgagag ccacttcaat ctgaccgtgg
     1141 agagcgcggc gttcaccggt ctcggccgcc tggcgcgtca gcggctggtc atgcgcgttc
     1201 tggccgacga actggccgga ccggtgcatg cgctgtcgat tcgcgccctc gcgccagggg
     1261 aatagctccg ttcgtttggg ctgtccccta acccgctctt ccccgcgaaa gcggggatcc
     1321 agtggtgtcc agcttgacgc acttccaatg ggattggcct gcctcaggca aacgaaaaaa
     1381 gcctgggtcc ccgctctcgc ggggaagagc gggacttgag tgctctgcgt cgtgccgact
     1441 ctcccaatca aggggagaga ggcggagcct aggactccag ggccgttcgc agggcttggg
     1501 ccaattcccc ggtccggaat ggcttgccga gcacgggcga gccttggt
//
LOCUS       DATAQL010001141         8534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1336875, whole genome shotgun sequence.
ACCESSION   DATAQL010001141 DATAQL010000000
VERSION     DATAQL010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336875"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VHS97_19700"
     CDS             <1..568
                     /locus_tag="VHS97_19700"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX3450490.1"
                     /translation="AAMEGLFKSGPYAELAIIGQPDIVARELENPIVVPGALSFLAYG
                     TFGSTVKGLNDFPMDQWPGNIELLYYSYHIMVGLGTLFILVMGVATALLWRQRLTRTR
                     PMLWVLMLAFPFPYIATTAGWWTAELGRQPWVVYGLMRTADASSQQVNQGDVVFTTLG
                     FAGLYLLLGVLFVVHVLKDIDRGPVPLH"
     gene            583..1614
                     /gene="cydB"
                     /locus_tag="VHS97_19705"
     CDS             583..1614
                     /gene="cydB"
                     /locus_tag="VHS97_19705"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX3450491.1"
                     /translation="METAWFSIVSAMLAIYVVLDGFDFGVGIVHHFVARTDQERRTVL
                     SAIGPVWDGNEVWLIAAGGVLFMAFPRTYSTAFSGFYLALMIVLWLLILRGVAIEFRS
                     HQENPLWREFWDTVFSLASMLLAVVFGTSLGNLVRGVPLGMYGLSGMPLFTKFVPGKE
                     AGIFDWYTTLVGLFTLCALAGHGALYLVWRTTGLVQARSLTCALRMWRAVLPLWIAVT
                     IATAWVQQEVFTNLFARPWSIAFVLPSLGGFCAVYYFLRHGRDLAAFLSSSVFLLGLI
                     GATMIGNYPYWLRSTIDSSFSLSAANTISAGYGLRVALVWWIVGITLACGYFVYLYRS
                     IRGKVEVEI"
     gene            1674..2042
                     /locus_tag="VHS97_19710"
     CDS             1674..2042
                     /locus_tag="VHS97_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450492.1"
                     /translation="MITKGVVEESTTFDVARRQRLEYVLCSAPDSPAVTATFDDGRVV
                     VRVPTEQVRAWGVTDQVAINAVQTASDGGVLKILIEKDFECIDAPVGESQDDAFRRPD
                     MGAACSSTAIPENVNTNEHA"
     gene            2098..5055
                     /gene="fdhF"
                     /locus_tag="VHS97_19715"
     CDS             2098..5055
                     /gene="fdhF"
                     /locus_tag="VHS97_19715"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX3450493.1"
                     /translation="MSSVVIDGSVQKAKAGERLIDVIARVGIELQAVCYHAQLGPLQT
                     CDTCMVEVDGKLIRACAALVSEGMAVSTRSAAAAAAQREAFDRVLANHLLYCTVCDNN
                     NGNCTVHNTTKLLAVEHQQIPFQPKPYEVDDTNPFYRYDPSQCLLCGRCVEACQNVEV
                     NETLSINWEDEHPRVLWDGGTTIGESSCVSCGHCVTVCPCNALMEKSMLGHAGFLTAL
                     PKAALEGMIDVVKGIEPEAGYGTILKVSEAESAMRQSRVRRTKTVCTYCGVGCSFEVW
                     TKDRHILKVEPEHGPTNGISTCVKGKFGWDFVNSTDRLTKPLIREGDQFRAASWEEAI
                     QLIAGRLSQIKAKDGPDSIAFISSSKCTNEESYLMQKLARAVVGTNNIDNCSRYCQAP
                     ATTGLFRTVGYGGDSGSISDIAQAGLVMIIGSNTAESHPVLATRVKSAHKLRGQKLIV
                     ADLREHEMARRADVFLRPQPGTDLVWLSAISRYLLDEGLAKTAFLDRWVNGLEDYRKS
                     LEPFTLDAAEQRCGLSVETLKKVAHMITEADGVCILWAMGVTQHTMASDTSTAISNLL
                     LITGNYMRAGAGAYPLRGHNNVQGASDHGAMPDKLPGYQSVDDPEVRAKFEAAWGVTL
                     PTAKGLDNHEMVDAIQQGKLKAMYLFGEEMSLVDSNSNYVQDAFAKLEFFIVQDIFFS
                     HTCRFADVVLPAAPSLEKEGTFTSTERRIQRLYQVLEPIEGSRPDWRIVRDVANHLGA
                     NWGYEHPSEIMDEVASLTPLFAGVSYDRLEGYKSLQWPVAADGTDEPLLYTERFAFPD
                     GKATLFPLSWSEPTDQPDAEFDLHLNNGNLLEHFHEGNMTYRVEGIRQMTPDVFVEIS
                     PELAQERGIQSGTRVELISRYGRVSVRALVTDRVHGKQLFMPMNSTESPVNRLIGSHT
                     DAVTHTPAYKEAAVRMLVLPEVDESPLPRVNPRFGQPTPQWGVQVERKWNRGGYSVPE
                     VRLVQIQNGQSKE"
     gene            5061..5537
                     /locus_tag="VHS97_19720"
     CDS             5061..5537
                     /locus_tag="VHS97_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450494.1"
                     /translation="MARPITLELPTCDPRAELRSRLQTAPAEHAEALLAAFEVLQGLH
                     DRGVLDLMRGALGSSDKVLEIAVDVAKSPESIRSIRNMLLAVNLLSAIDPEELITLTQ
                     AVPQALKLMVRQPEPPGLWQLMRNFLWNQNIRRGLSALNVLLETFGGTLARKSGHE"
     gene            5627..6457
                     /gene="fdhD"
                     /locus_tag="VHS97_19725"
     CDS             5627..6457
                     /gene="fdhD"
                     /locus_tag="VHS97_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX3450495.1"
                     /translation="MLILRWDGEIAREQPDELVDEEPLEIRIQGRAISVTMRTRGHDV
                     DLAAGFLLTEGIIHRHQDVISIKHCAHNQGHNVLNVVLAPGVSVDFRRLTRHLFASSS
                     CGLCGKATIESVHGQFPSVRSDIVIDAELLATLPEKMRAAQATFERTGGLHAAALFDP
                     AGRLIVLREDVGRHNAVDKVVGRCLLNGQLPLDEHVLVVSGRSSFEIMQKALAARVPV
                     VAAVSAPSSLAVEFAAESGQTVIGFLRDHRMNVYSHPHRISFGRIAKSHLAQGEAGRT
                     "
     gene            complement(6459..7535)
                     /locus_tag="VHS97_19730"
     CDS             complement(6459..7535)
                     /locus_tag="VHS97_19730"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX3450496.1"
                     /translation="MPILIAGIGTSVPPHRIAQGDAAEIARPYSCETTAHERVLQAIY
                     NGTGVETRHSVILQASDGCLAGRQSFYGDAIPTTRDRMRVYEQEASTLALAAAKQALI
                     DSQVAPDRITHLVTVSCSGFYAPGFDIALIKRLPLAANVARTQVGFMGCHGALNGLRV
                     AQAFVAADPGACVLLCAVELCSLHHQYGWNAEQIVANSLFADGAAAIVALSGTGPADT
                     LPQIVAAGSTLIDESEDAMSWRIGDHGFQMTLSARLPELIARNLRGWLDGWLYAHGLT
                     VAAVGSWAVHPGGPRILAAVAESLGIERRALDLSHSVLAEYGNMSSPTVLFILERLRQ
                     TKAKRPWVAIGFGPGLAVEVVLLA"
     gene            complement(7523..>8534)
                     /locus_tag="VHS97_19735"
     CDS             complement(7523..>8534)
                     /locus_tag="VHS97_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450497.1"
                     /translation="NAHALACLRAAGLGSLVERQGAIKLDQLQVGFRNRRVRLDLPEG
                     AALSRSRLDAALVDAAVDAGTQFVPETQAIVGGIRDGLRLLRLSHRGKSIDIRGRVVL
                     VAAGLGHHCQTDSATARTQVRPGSRIGAGCLIDDAPGFYHERTIFMAVGRAGYVGAVR
                     VEDGGLNVAAAFDPAVVRRHGAPGAAAAAIILDAGFPAIAGLERALWQGTAGLTRHTQ
                     PLAQERLFLLGDAAGYVEPFTGEGIGWALASARAVAPLAQRAMGNWDPRFARDWSELY
                     RQLIGRRQLVCRAVAMGLRRPWLAAIGFEVLTRVPASAGLVVRRVNAPSPFTNASSAC
                     QS"
BASE COUNT         1663 a         2609 c         2659 g         1603 t
ORIGIN      
        1 cgccgcgatg gagggattgt tcaaaagtgg gccgtatgcg gaactcgcca tcatcggtca
       61 gcctgacatc gtcgcccgcg agctggaaaa cccgatcgtc gtgcctggtg cgctcagctt
      121 tctcgcgtac gggaccttcg gcagcaccgt caagggactg aacgactttc cgatggacca
      181 atggccgggc aacatcgaac tgctctatta cagctaccac atcatggtcg ggttggggac
      241 gctcttcata ctggtgatgg gagtcgccac tgcgctcttg tggcgtcaaa ggctcacacg
      301 gacgcgtccg atgctgtggg tcttgatgct ggcgtttcct ttcccgtaca tcgccacgac
      361 cgcggggtgg tggaccgcgg aactaggacg gcagccctgg gtcgtatacg gcctgatgcg
      421 tacggctgac gccagctcgc agcaggtcaa tcagggcgac gtggtcttta ccaccctggg
      481 ctttgccggg ttgtacttgc tcctcggagt tctctttgta gttcatgtcc tcaaggacat
      541 cgatcgaggc cccgttccgc tccactagac gaggattctt cgatggaaac ggcttggttc
      601 tcgatcgtat cggcgatgct cgccatctat gtcgtgctgg atggtttcga tttcggagtc
      661 ggcattgtgc atcacttcgt cgccagaacc gaccaggagc ggcggacagt gctgtcggca
      721 atcggcccgg tctgggacgg caacgaggtc tggctgattg ccgcgggagg ggtgttattc
      781 atggcctttc cgaggacgta ttccacagca ttcagcggtt tttacctagc tctcatgatc
      841 gtgctctggc tactgatcct gcgcggggtg gcgatcgaat tccgatcgca tcaagaaaat
      901 cccctctggc gcgaattctg ggatacggtg ttttcgctcg cgtcgatgct tttggccgtc
      961 gtgtttggca cttccctcgg taatctcgtg cgcggtgtgc cgttggggat gtacgggcta
     1021 tcgggcatgc ccctgttcac gaaattcgtg ccggggaaag aggccggcat tttcgactgg
     1081 tatacgacgc ttgtggggct ctttacgctt tgtgcactgg cggggcacgg cgcgttgtac
     1141 cttgtctgga ggacgaccgg cctcgttcaa gctcgaagcc tgacttgcgc tctgagaatg
     1201 tggcgggccg tccttccgtt gtggatcgcc gtgactatcg caacggcgtg ggttcagcag
     1261 gaagtcttca ccaacctgtt cgctcgaccc tggtccatcg cttttgtgct gccgtcgctc
     1321 ggcggatttt gtgcggtcta ttactttctc cggcacggac gagacctggc agcgttcctg
     1381 tcatcgtccg tattccttct cggactcatc ggggcaacga tgatcgggaa ttacccgtat
     1441 tggctgcgtt cgaccatcga tagctcgttc agtctttccg ccgcgaacac catctcggcg
     1501 ggctacggat tgcgagtggc tcttgtctgg tggatcgtcg gcatcacact ggcatgcggc
     1561 tattttgtct acttgtaccg ttccatccga ggaaaggttg aagtcgagat atgaagctaa
     1621 gaatcagagg caactcgatc cggttgcggc tcggacagtc ggaagtcgcg cgaatgatca
     1681 cgaagggagt tgttgaggag tccacgacgt tcgacgtcgc tcgacggcag cgcctggagt
     1741 acgtcttgtg ctcagcccct gactctccgg ccgttaccgc gaccttcgac gatggtcggg
     1801 tcgtcgttcg cgtgccgacc gagcaggtcc gcgcatgggg cgttacggac caggtggcga
     1861 tcaacgccgt ccaaacggct tcggacggag gggtcttaaa gatcttgatc gagaaagact
     1921 tcgagtgcat cgacgctcca gttggcgagt cgcaagacga cgcctttcgg cgccctgaca
     1981 tgggcgccgc atgctcgtcc acggcgatcc cagagaatgt taacacgaat gaacatgcat
     2041 aatgactcta gcgtgaaccc gcgcccgggc ccgcaaccag aacaggagtg gtcgacaatg
     2101 agcagcgtcg tgattgacgg ctccgtccag aaggcaaagg ccggcgaacg cttgatcgac
     2161 gtcatcgccc gcgtcggcat cgaactgcag gcggtgtgct accacgcgca gctgggcccg
     2221 cttcaaacct gcgacacgtg catggtcgaa gtggacggaa agctcatcag agcctgcgcc
     2281 gcccttgtga gcgaaggcat ggcggtgtcg acaaggtccg ccgccgcggc ggccgcacag
     2341 cgggaggcgt tcgaccgggt cctcgctaac cacttgctct attgcaccgt ctgcgacaac
     2401 aacaacggca actgcaccgt ccacaacacg acgaaactgc tcgcggttga acatcagcag
     2461 atcccctttc aaccgaagcc gtacgaggtg gacgacacta accccttcta tcggtatgat
     2521 cccagccagt gcctcctctg cgggcgctgc gtcgaggcgt gccagaatgt tgaggtcaat
     2581 gagacactct cgatcaactg ggaagacgaa catccccgcg tattgtggga tggtggcaca
     2641 acgatcggcg aatcgagttg cgtctcctgc ggccactgcg tcacggtctg cccctgcaac
     2701 gccttgatgg aaaaatcgat gctaggacac gccggcttct tgacggccct accgaaagcg
     2761 gcgctcgagg gcatgatcga cgtcgtgaag ggcatcgagc cggaggcggg ctatggcacg
     2821 atcctcaagg tgtcggaggc cgagtcggcc atgcggcagt cacgcgtgcg ccggacgaaa
     2881 acggtttgca cgtactgcgg tgtcggttgc agtttcgagg tctggacgaa ggaccgccat
     2941 attctcaagg tcgaacccga gcatggcccg accaacggaa tctcaacctg cgtcaaggga
     3001 aagttcgggt gggacttcgt caatagcacc gaccgcctta ccaaaccctt gatccgcgag
     3061 ggagaccagt tccgcgccgc cagctgggaa gaagccattc aactgatcgc cggccgactg
     3121 tcgcagatca aggcgaagga tggtcccgac tcgatcgctt tcatctcctc atccaagtgc
     3181 acgaacgagg agagctatct catgcagaag ttggcgcgtg ccgtcgtcgg cacgaacaac
     3241 atcgacaact gctcccgata ttgccaggcg ccggcgacga cggggctgtt tcgtaccgtc
     3301 ggctatggcg gagattcggg ttcgatctcc gatatcgccc aggccggcct tgtgatgatc
     3361 atcgggagca acacggcgga gagtcacccg gtgctcgcga cccgggttaa gagtgctcac
     3421 aagctccgcg gccagaagct gatcgtcgcg gacctgcgcg agcacgaaat ggcgcggcgg
     3481 gccgacgtct ttctgcgacc tcagcccggt accgaccttg tgtggttgtc ggcgatcagc
     3541 cgctacctcc tcgacgaggg cttggcaaag acggcgtttc tcgaccgctg ggtgaacggc
     3601 ctggaagact accggaagag cctcgaaccc ttcacgctgg atgccgccga gcagcgatgc
     3661 ggactgtccg tcgagacact gaagaaagtc gcacacatga tcacggaggc ggacggcgtc
     3721 tgcatcctgt gggccatggg agtcacgcaa cacacgatgg cttcggacac atcgacggcg
     3781 atctcgaacc tgctgctgat caccgggaac tacatgcgag cgggagccgg cgcgtatccg
     3841 ttgcgcggtc acaataacgt acaaggggcg agtgatcacg gagccatgcc agacaaactt
     3901 cccgggtatc aatccgtgga cgatccggaa gtgcgggcaa agttcgaggc ggcttggggc
     3961 gtcacgctgc cgaccgcaaa ggggctcgac aaccacgaga tggtggacgc gatccaacag
     4021 ggaaagctca aggcaatgta tttatttggt gaagagatga gcctggttga ctcgaactcg
     4081 aattacgtcc aggacgcctt cgccaagctc gaattcttca tcgttcagga catcttcttc
     4141 agccacacct gtcgcttcgc ggatgtcgtg cttcccgccg cccccagcct ggaaaaggag
     4201 ggaacattca ccagcaccga gaggcgaatt cagcggctat accaggtgct cgaaccgatc
     4261 gaagggagcc ggcccgactg gcggatcgtc cgcgacgtgg cgaaccacct cggtgcgaac
     4321 tggggatacg aacatccgtc cgagatcatg gacgaagttg cgtcgttgac cccactcttt
     4381 gccggcgtct cttatgatcg gctcgaggga tataagtcac ttcaatggcc ggttgccgcc
     4441 gatggcaccg atgagccgct tctttatacc gagcggtttg cctttcccga cggcaaggct
     4501 acgctcttcc ccctgtcctg gtccgagccg acggaccagc ctgacgcgga attcgacctg
     4561 cacctgaaca acggcaacct gctcgagcac ttccacgagg gaaatatgac gtaccgagtc
     4621 gagggaattc ggcagatgac ccccgacgtg ttcgtcgaga tatctcccga gctcgcacag
     4681 gagcggggga tccagagcgg aactcgcgtg gaattgatct cgcgctacgg ccgcgtgtcc
     4741 gtgcgagccc tggtgaccga tcgggtccac ggaaagcagc tcttcatgcc gatgaactcg
     4801 acggaatcac cggtgaatcg gttgattggc agtcacacgg acgccgtgac gcacacgccg
     4861 gcgtacaagg aagcggccgt gcgaatgttg gtgttgccgg aagtggacga aagcccgctg
     4921 ccccgcgtga acccccgctt cgggcaaccc acgccccagt ggggcgtcca ggtcgaacgg
     4981 aagtggaacc gtggtggcta ttccgtcccg gaggtccggt tggtgcaaat tcagaacgga
     5041 cagagcaagg agtgaagacc atggctcgac ccatcacgtt ggagttgccc acctgtgatc
     5101 ctcgcgcaga gctgcggtct cgactacaga ctgctccggc ggagcacgcc gaggcgttgt
     5161 tggccgcctt tgaagtgctt caaggtctgc acgaccgcgg cgtactcgac ctcatgcgcg
     5221 gtgcgctggg atcgagcgac aaggtcctcg agatcgcagt ggacgtcgcg aaatctccgg
     5281 aatcgatccg gagcatccgc aacatgctac ttgcggtcaa cttgctcagc gcaattgatc
     5341 cagaggagct cataaccttg acacaggcag tgccgcaagc cctgaagctg atggttcggc
     5401 agccagaacc gccggggttg tggcaattga tgcggaattt cctctggaac cagaacatta
     5461 ggcgtggcct gtccgctctc aacgttctgc ttgagacgtt cggcggcacc ttggcccgca
     5521 aaagcggaca cgaatgacga aaatttcgtt attcgaaacg atgaaacggg caagaaactg
     5581 gcttggatag tgtggccaag acaggccgcc gcacggggag cttgccatgc tgattctgag
     5641 gtgggacggt gaaatcgctc gcgagcagcc ggacgaactc gtggatgagg aaccgctgga
     5701 gattcgcatt caaggcaggg cgatcagcgt caccatgcgc acccgcggcc atgacgtcga
     5761 tctggccgcc ggattcctcc tcacggaggg catcatccat cgtcaccagg atgtgatctc
     5821 cattaaacac tgcgctcata accaagggca caatgttctc aacgttgtgc ttgctcccgg
     5881 tgtctccgtc gacttccgtc gactgacccg ccacctcttc gccagttcga gttgcgggct
     5941 gtgcggaaag gccactatcg agtccgtcca cggacagttc ccgtcggtcc ggtcagacat
     6001 tgtgatcgac gcggaacttc tcgcgactct gcccgaaaaa atgcgggcgg cccaggcaac
     6061 cttcgagcgg accggcggcc tgcatgcggc tgcgctgttc gatccggcag gacgactgat
     6121 agtcctccgc gaggacgtcg gccggcataa cgcggtcgac aaggtggtcg gccgctgcct
     6181 gctaaacggc cagctccctc tcgacgaaca tgtcctggtt gtctccggtc gcagctcgtt
     6241 tgagattatg cagaaggcac tggcggcacg cgtgccggtg gtcgccgccg tgtccgcgcc
     6301 ctccagcctt gcggtggaat tcgccgcgga aagcggacag acggtgatcg gcttcctccg
     6361 agaccatcgg atgaacgtat actctcatcc gcatcgaatc tcattcgggc gaatcgcgaa
     6421 aagtcattta gcgcaggggg aggcgggtcg cacttagcct acgcgagcag caccacctcg
     6481 accgcgaggc ccggtccgaa gccgatggca acccacgggc gcttggcctt ggtctgacgc
     6541 agccgctcga ggatgaaaag aacggtcggc gacgacatgt tgccatactc ggccaagact
     6601 gagtgcgaaa gatcgagcgc ccggcgctcg ataccgagtg attccgccac ggccgccagg
     6661 atgcgcgggc cccctgggtg cacggcccat gagcccaccg cggccacggt cagcccgtgc
     6721 gcgtaaagcc agccatcgag ccacccgcgc aggttgcgcg cgatcagctc ggggagacgc
     6781 gcggagagcg tcatctgaaa gccatggtcg ccgatgcgcc aggacatcgc atcttcggac
     6841 tcgtcaatca aggtcgagcc cgccgcgacg atctggggta gagtatccgc gggacccgtt
     6901 cccgagagag cgacgatggc ggccgcaccg tcggcaaaca gcgagttggc cacaatctgt
     6961 tcggcattcc agccgtactg atgatgaagg ctgcagagtt cgacggcgca caagagcacg
     7021 caggcccctg ggtcggcggc gacaaatgcc tgagccacgc gcaggccgtt caaggcgcca
     7081 tggcagccca tgaatccgac ctgcgtgcgt gcgacgttcg ccgccagggg caatcgcttg
     7141 atcaatgcga tgtcaaagcc gggcgcatag aaaccgctgc acgagacggt gaccaggtgc
     7201 gtgatccgat cgggagcgac ctgtgagtcg ataagggcct gcttggccgc ggcgagtgcc
     7261 aaggtgctcg cctcttgttc gtatacccgc atacggtcgc gcgtggtggg gatcgcgtcg
     7321 ccataaaacg actgtcgccc ggccaagcaa ccatccgatg cctgtaagat cacgctgtgg
     7381 cgcgtttcca cgccggttcc attgtaaatg gcctgcagca cacgctcgtg agcggtcgtc
     7441 tcacacgagt acggccgggc gatctccgcg gcgtcaccct gggcgatgcg gtggggcgga
     7501 acggatgtgc cgatccctgc gatcaagatt ggcatgcaga gctcgcattc gtgaagggcg
     7561 acggggcgtt gactcggcgc acgaccaatc cggccgacgc cgggacgcga gtcaatactt
     7621 caaaaccgat cgcggccaac cacggccggc gcaatcccat cgccaccgcg cgacagacta
     7681 gctgacggcg accgattaat tgccgataaa gctccgacca gtcccgcgcg aatcgcgggt
     7741 cccagtttcc catcgcgcgc tgggcaagcg gcgcgacggc ccgggccgac gccagggccc
     7801 agccgattcc ttcgccagtg aatggttcga catatccggc ggcatccccc aacagaaata
     7861 accgttcctg ggcgagcggc tgggtatgcc gcgtgaggcc ggccgtcccc tgccagagcg
     7921 cacgctcgag cccggcgatc gccgggaagc cggcgtcgag aatgatcgcc gccgcggcag
     7981 cccccggagc gccgtggcgc cggaccactg ccgggtcgaa agcggccgcc acgtttagac
     8041 cgccgtcctc gacccttacc gcgccgacat accccgcgcg tcccaccgcc atgaagatcg
     8101 tccgttcgtg gtagaaaccg ggagcatcgt cgatcaagca acccgcgccg atccgcgagc
     8161 ccggccggac ctgggttctg gccgtagcgc tgtcggtctg gcaatggtgt cctagtccgg
     8221 cggccactag cacgactcgc cctcttatgt cgatcgattt tcctcgatgg gagaggcgca
     8281 gcagccgtag cccgtcgcgg ataccgccga caatcgcctg cgtctcgggc acaaactggg
     8341 ttccggcatc tacagccgca tcgacaaggg cggcgtcgag ccgcgatcgg gaaagcgccg
     8401 ctccttcagg caagtcaagg cgaacccgac ggttgcggaa accaacctgc aactggtcga
     8461 gcttgatcgc tccctgccgc tccaccagtg atcccaatcc ggcggcgcgc aggcatgcga
     8521 gcgcatgggc gttc
//
LOCUS       DATAQL010001142         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4695353, whole genome shotgun sequence.
ACCESSION   DATAQL010001142 DATAQL010000000
VERSION     DATAQL010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4695353"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..816)
                     /locus_tag="VHS97_19740"
     CDS             complement(13..816)
                     /locus_tag="VHS97_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450498.1"
                     /translation="MCKSGKHSLRFLLVSIISLFVADMPTRAQDPELAFDQVVGHPRG
                     LPQLPPQGAWGEVINVTTRWIVIQNHSGQQYPIAAEDVGEFLVRWPSSVENLGPQSMV
                     EAVGREAGSNVVEVEHVDVFEGNDRTLVAPTYNSLLPNNAVVTTVDPGFNRFMNAWDY
                     GGQNQLYGWAYPTGLVGSDIPIRLHVVGSVIQRVPLRLSILGNNVATVAPGDNLQFTV
                     SQVTRGSLDYARKGDYAFLMPRQLNPKGLVLSQLVLYKSIPFSQFSLTR"
     gene            1197..>2520
                     /locus_tag="VHS97_19745"
     CDS             1197..>2520
                     /locus_tag="VHS97_19745"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3450499.1"
                     /translation="MSTVDRRSSENTAWASDGDPNSHDLTGVTLGDFQVEKLLGQGGM
                     GDVYLATQLSLNRPVALKVLRSNFSSNPTYLARLRSEGTAVAKLNHPNIVHVYTLGCI
                     DQINFIAMEYVQGTNLKEYFIKKGALDLPLAFSIMRQTGQAVGAAGEVGLVHRDIKPE
                     NILVTKKGRVKVADFGLCRDQESGSPHLTQHGSTMGTPLYMSPEQAQGHVTDHRSDLY
                     SMGVTFYHMLAGVPPFHADTALALALKQVREMPRSMLIHRPDLPPELDQLVLKLMAKR
                     AEDRYQSAALMLADLAKLRDTLHVGATAAVSGTSTSASTANENARTVSPSALSDPNKE
                     AAPRTSSIISGTVSAMRIPVSKFLGRLNPAVIAATSAVCLLAGAIAGWSARNPDLQAI
                     PAQSAAPLPALWLEPRWSAIPRQSTPEEQLRYALLQAPHEDWAPAFLAV"
BASE COUNT          533 a          743 c          745 g          499 t
ORIGIN      
        1 aagactgacc tactaacggg ttaggctaaa ctggctgaag ggaatcgact tgtaaagtac
       61 gagctgcgat agcacgagcc ccttcggatt aagctgcctc ggcatcagga aggcgtagtc
      121 tcccttgcgg gcatagtcga gtgagccgcg ggtgacctga ctgacggtaa attgcaggtt
      181 gtcgccgggg gcgacggtcg cgacgttgtt ccccaaaatg cttaacctga gtggcacacg
      241 ctgaatgacc gaaccgacca cgtgcaagcg aatgggaatg tcactgccga cgagaccggt
      301 cggatacgcc cagccgtaga gttggttttg cccgccataa tcccaagcgt tcatgaagcg
      361 attgaaaccc ggatcgaccg tggtcaccac ggcgttattg ggaagcaggc tattgtaggt
      421 tggggcgaca agagttcgat cgttgccctc gaagacgtcg acatgctcga cctcgaccac
      481 gttggagccg gcctcgcggc cgaccgcctc gaccatcgac tgaggtccga gattttcgac
      541 actgctcggc cagcggacca ggaactcccc cacatcctcg gcggcaatcg ggtactgctg
      601 tcccgagtga ttttggatta caatccatcg agtcgtcacg ttgatcactt cgccccacgc
      661 accctgagga ggaagctggg gaaggccgcg gggatggcca acaacctggt cgaaggccag
      721 ctccgggtct tgggcccggg tgggcatgtc agccacaaat aggctgataa tactgaccag
      781 caggaatcgg agcgagtgct tgcccgattt gcacatcgac tcacctccgg cgagtgcagg
      841 gaattctcaa gcgattgctt cagaatactc cgtcggcttg gtgtgacaca atgtcttctt
      901 cgtcggtttc gcgcgatctt tcgcgatact aaatcgagcg acatcgcaaa gtggataggc
      961 ggatttggcg gcgattattg acaggcagtt ctcaaaatcc actgccggga tacgagggtc
     1021 gaccggctga cggccgatca ctgtcggtgg aaacgtcgta gcaattccgc agcgagcgac
     1081 attgcccttt gccgagaatt cgtgatttcc gtaaactgta gtcggcacac agcgcatcca
     1141 acccgatgcc ggaacgcatc gtgcttctaa ctcttgagga aggtcgaccg ggggctatga
     1201 gcacagtgga tcggcgatct tccgagaaca ccgcctgggc cagtgacggc gatcccaatt
     1261 cccacgacct gacgggcgtg acgctgggtg attttcaagt cgaaaagctg ctgggccagg
     1321 gaggaatggg tgacgtttat ctggccactc aactaagctt gaatcgcccg gtcgccctca
     1381 aagtcctcag atctaacttt tcgtcaaatc caacctatct tgcccgattg cgctctgaag
     1441 gaacggcggt cgccaagctt aatcatccca atatcgttca cgtatacact ttaggttgca
     1501 tcgaccagat caactttatt gcgatggagt acgtccaggg caccaacctc aaggagtact
     1561 tcatcaagaa gggcgcgctc gatctaccgc tggcgttttc gatcatgcgg caaaccggtc
     1621 aagccgtcgg tgcggctggg gaagtcggtc tggtgcaccg cgatatcaag cccgagaaca
     1681 tcttggtcac caagaaggga cgggtaaagg tcgcggattt tgggctttgc cgcgaccagg
     1741 agagcggtag cccccatctc acgcaacacg gctcgacgat gggtacgccg ttgtacatga
     1801 gtcccgagca ggcccagggg cacgtcactg atcatcgtag cgacctgtat tccatgggtg
     1861 taacgtttta ccacatgctg gcgggtgttc ctccttttca cgcagacaca gcgctcgcgc
     1921 tcgcgctcaa acaggtgcgg gagatgcccc gcagcatgct tatccatcgg cccgacctgc
     1981 cgccggagct cgatcaactt gtgctcaagc tgatggctaa gcgtgcggaa gaccgctacc
     2041 agtcggcggc ccttatgctc gccgacctgg cgaaactgcg cgatacgctc catgtgggcg
     2101 cgaccgcggc cgtttccggc acgagcacat cggcctcgac ggcgaacgag aatgcgcgga
     2161 cggtaagccc gagcgcatta tccgacccga ataaagaggc ggcaccgcgg accagttcga
     2221 ttatatcagg caccgtgagt gccatgcgta ttccggtcag caagttcctg gggcggctca
     2281 accccgcagt gatcgccgcg acaagcgcgg tttgcctgtt ggcgggggca atcgccggtt
     2341 ggagcgcgcg aaacccggac ctgcaagcca tcccggccca atccgccgcg cccttgccgg
     2401 ccttatggct cgagccgcgc tggtccgcga tccccagaca aagtaccccc gaagagcagt
     2461 tgcgatatgc gcttctgcaa gcgccccacg aggattgggc tcccgcgttt ctcgccgtcg
//
LOCUS       DATAQL010001143         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3933669, whole genome shotgun sequence.
ACCESSION   DATAQL010001143 DATAQL010000000
VERSION     DATAQL010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933669"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..2663)
                     /locus_tag="VHS97_19750"
     CDS             complement(378..2663)
                     /locus_tag="VHS97_19750"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HEX3450500.1"
                     /translation="MTTCNPIPVRGWTIALLCGITALGARPDDSKTKSDAPTAEAVLR
                     RTADYYKTLKSFAVDIEREQTIGPRSMKSSLVAATERPNRLAIHGKGAGLGIDVVCDG
                     KTLSVSIPAVKRYTQSKAPASLSEMSGDAMLQSVLMASLQGTLLVELTAADPYKALME
                     GVKTSAYLGDEVLDGARTHHVKCTQDQFDWEVWVAVEGDPLLRRVVLDMTKSMANTPA
                     AAQFKGQRLELVQTFKGWKVDTALDTKTFAFEPPQGSQKADNLMEALTGGRRQASSPL
                     VGKPAPEISLKLLDNGEFRLNDHRDKDIVMLDFWATWCGPCVQELPLLAEVAESYKGK
                     GVAFFAVNLRETPEQIKKFQDDKKLKFTVAIDPDGAVGTAYGAQAIPMLVLVDKKGVV
                     QVVHVGYNPAIKTTLANELDALLAGKDLAKEAAERAKAAVAKSEGLELVWTVSGPYAS
                     VATDPRGRSIYAIGARGRCDVLDISGKTTRTLPMAVATQGVTVRVGRQVGGSEGFLIF
                     RAWGPSVAALKGDGTKLWEEAGGQGIDDVWAADLNGDGADEAIVGYNGATGLHVFSPD
                     GKRLWTRTDMGNVWNVTAGDLDGDGKLEVVSTSAQGKVHVFAARDGTPLMTLDPGIYA
                     TKVRVATGRAKTAPKGDLLLVAGSDSAQHGEALVALGGDGKVLWTTKLPAAVATCQAM
                     TVSPDGALAALAFQGGRICVVDVGSGRIVGQMADQGFLPSVAWATRAGGADNLLLVAA
                     QDAVNAFQVKPPAASARNDRP"
     gene            complement(2834..3679)
                     /locus_tag="VHS97_19755"
     CDS             complement(2834..3679)
                     /locus_tag="VHS97_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450501.1"
                     /translation="MITRPWPREWADPHDSNEISVLPLIATESRRNQPGWCTYVRAML
                     DAPEIEVFCGGINAKTSTAAALWRQGNLLHFGFDLSPDEMNEWGRSMLVNAIAYISRF
                     TDDRPIMQTPSPFAGQEFIARRHIENVIGRGDGFWDYLKSYFDQDALRTAGVTNLAAF
                     AKWYPTVRDCVSPNAEGLLRVDGDAQAMQLYPGSREFFDRAIGDLARPGNEAERARRM
                     LERYAPEGPGRAAKAQAWADWWRANADYLFFGEAGGYRWYLDPLAKARGVPTSRLRGQ
                     ARASR"
BASE COUNT          675 a         1262 c         1197 g          740 t
ORIGIN      
        1 aagaggaaat tgcgcgcgta ggtcgggtat gctcgcgggc taaagggcgg tcgcgtcagc
       61 cgggcagata aagctgggaa cgaccgggcc ttgactgagc caagcgcgac gacggcgtcg
      121 aacatcgcgg cgtagatctg attgatgacg atggacacgc tgatcgttca gtactggcca
      181 accgggtggg aacggaatga aatccggcta ttccccagac ggggtgaatt tcgcgccctc
      241 tcaatcctcc gaatttgatt aggcggttgt gttgtagctt ggtaaagccc ttcgcaaggc
      301 ggtttcgaac agccgcatcc atcaacaagc aggtgccact tcactcgctc ccagctggct
      361 ctcgggctgc cagcaattca cggccggtca ttcctggctg aggcagcggg cggcttcacc
      421 tggaatgcgt tgaccgcgtc ctgggcggcc acgagcagca ggttgtccgc accacctgcg
      481 cgggtcgccc acgcgaccga aggaaggaag ccctggtcgg ccatttggcc cacgatccga
      541 ccgctaccga cgtcgaccac gcagatccgg ccaccctgga acgccaatgc cgccagcgcc
      601 ccgtcgggtg agaccgtcat tgcttggcat gtcgcaaccg cggccggtaa cttcgtcgtc
      661 cagagtacct tgccgtcgcc gccgagcgcc acgagtgcct cgccatgttg tgcggagtcg
      721 ctcccagcca cgagcaagag gtcgcccttg ggtgcggtct tcgcccgacc tgtggcgacg
      781 cggaccttgg tggcgtaaat ccccgggtcg agcgtcatca agggcgtgcc gtccctcgca
      841 gcgaaaacgt ggaccttgcc ctgcgcggat gtgctcacaa cctcgagctt gccatcgccg
      901 tcgaggtcgc cggcggtgac gttccacaca ttacccatgt cggtccgcgt ccaaagtcgc
      961 ttgccatcgg gcgagaaaac atgcaggcca gtcgcgccgt tgtacccgac gatcgcctcg
     1021 tccgccccgt cgccgttcaa gtcggcggcc cagacgtcgt cgataccttg gccgccggct
     1081 tcctcccaga gttttgtccc atcacccttg agcgcggcga ccgacgggcc ccaggcccga
     1141 aagatgagaa aaccctctga gcccccgacc tggcggccaa cacggacggt gacgccttgc
     1201 gttgccaccg ccatgggaag ggtccgggtc gtcttgccac tgatgtcgag cacatcgcac
     1261 cgtccccgcg ctccgatcgc atagatcgac cgcccccgcg ggtcggtcgc gacgctcgca
     1321 tacggcccgc tgacggtcca gacgagctcc agtccttcgc ttttcgccac agcggctttc
     1381 gctcgttccg ctgcctcctt cgcgaggtcc ttgccagcga gcaaggcgtc gagttcgttc
     1441 gcgagggtcg tcttgatcgc cgggttatag ccgacatgga ccacctgcac cacgcccttc
     1501 ttgtcaacca gcaccagcat cggaatcgct tgggccccgt acgcggtgcc aacagcgccg
     1561 tcggggtcga ttgcgacagt gaacttcagc ttcttatcgt cctggaactt tttgatctgc
     1621 tcgggcgtct cgcgcaggtt gaccgcgaag aaggcgaccc ccttgccctt gtaggattcg
     1681 gcgacttcgg caagtagtgg cagttcctgg acgcacgggc cgcaccaggt ggcccagaag
     1741 tcgagcatca caatgtcttt gtcgcggtgg tcgttgagcc ggaattcgcc gttgtccagt
     1801 aacttcaggc tgatctccgg agcgggcttg ccgaccaggg gcgagctcgc ctgccggcgg
     1861 ccgcctgtaa gtgcttccat gaggttgtcc gccttctgcg agccctgtgg gggctcgaat
     1921 gcgaacgtct tcgtatcgag cgcggtgtct accttccagc ccttgaaggt ctggacaagc
     1981 tcgagcctct gacccttgaa ttgcgccgcg gcgggagtgt tggccatgga tttcgtcatg
     2041 tccaggacaa ctcttcgcaa gagtggatcg ccttcgaccg cgacccagac ctcccagtca
     2101 aactggtcct gggtgcactt cacgtggtgc gtcctggcgc cgtcgaggac ctcgtcgccc
     2161 aggtaggcgg acgtcttgac cccctccata agcgccttat acgggtcggc ggcggtgagc
     2221 tcgacaagca atgtgccctg gagggacgcc atgaggacgc tttggagcat cgcgtcgccg
     2281 ctcatttcgg agagcgaggc cggcgccttg ctctgggtat acctcttgac tgcggggata
     2341 gacacagaca gtgtcttgcc gtcgcagacc acgtcaatgc cgaggccggc ccctttgccg
     2401 tggatcgcca gcctgttggg ccgctcggtg gccgcgacaa gtgaactctt catcgacctc
     2461 ggaccgatag tctgctcgcg ctcgatgtcg acagcgaagg attttaacgt cttgtaataa
     2521 tcggccgtcc gccggaggac tgcctcagcc gtgggggcat ctgacttcgt cttcgagtcg
     2581 tccggacggg ccccgagggc cgtgataccg cagagaagcg cgatggtcca gccacggacc
     2641 ggaatggggt tgcaggtagt cacagcatcg tctcctcaac gcttttggga acaggttgga
     2701 ccgccatcgg tagaacccgt cgattcggga gtggcatcga actattatca tgcgcgtacg
     2761 ccgatcggcc cgcaaggagt cacagcccca agtgttcgcg attcgacaac atcgcgtgag
     2821 ccatctgccc gaatcaacgg ctcgcccgcg cctgcccgcg caatcggctg gtcggcacgc
     2881 cgcgcgcctt ggccagcggg tcgagatacc agcgatagcc gcccgcctct ccgaaaaaga
     2941 gatagtccgc gttggccctc caccaatcgg cccatgcttg tgcctttgca gcccgaccag
     3001 gcccttcggg agcataccgc tccagcatcc ggcgggcacg ctcggcttcg tttcccggcc
     3061 gggccaggtc gccgatggcg cggtcgaaga actcgcggct gcctgggtaa agctgcatcg
     3121 cctgagcatc gccgtcaacc cgcaagaggc cctcggcgtt gggcgagaca caatctcgga
     3181 ccgtcggata ccatttcgcg aaggccgcca ggttcgttac gccagctgtg cgcagggcgt
     3241 cttggtcgaa gtacgacttg aggtagtccc aaaagccatc gccgcgcccg atgacgtttt
     3301 cgatgtggcg cctcgcaatg aattcctgtc ccgcgaacgg cgatggggtc tgcatgatcg
     3361 gccgatcatc cgtgaaccgg gaaatatacg cgattgcgtt aacgagcatg gaccgtcccc
     3421 actcgttcat ctcatccggg gagagatcga agccgaagtg gagcaggttc ccttggcgcc
     3481 agagcgccgc ggcggtcgac gtcttggcgt tgatcccgcc gcagaagacc tcgatctcgg
     3541 gggcgtcgag catggcgcga acatacgtgc accatcccgg ctgattcctt cgtgactcgg
     3601 ttgcgattag cggcaagacc gagatctcat tcgaatcgtg tgggtcggcc cattcacgag
     3661 gccagggccg cgtgatcatc ttgcctcgat caatgactgt gggcgaacga aagatcgggt
     3721 ggtcgcgtaa cacgtacgcg aaaggttcca agcacgttca cccgctcccc ccgaccacct
     3781 tccagggtgc ggcgagcagg tgcccggcgc tgccgagcaa taccgtcggc ttggtccacc
     3841 ggctccgctc accgagcggc gatttcaggt cgct
//
LOCUS       DATAQL010001144         4403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2253181, whole genome shotgun sequence.
ACCESSION   DATAQL010001144 DATAQL010000000
VERSION     DATAQL010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253181"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1655
                     /gene="glnA"
                     /locus_tag="VHS97_19760"
     CDS             243..1655
                     /gene="glnA"
                     /locus_tag="VHS97_19760"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX3450502.1"
                     /translation="MTPKEVLAQIRQREVTTVDLRFMDFPGVWQHFSIPADALTEETF
                     EEGIGFDGSSVVGWRAINESDLLVVPQPETALIDPFPAQPTLTMICNIHDPITHQDYT
                     RDPRNIARKATSYMRNTGVADQCLLAPELEFFVFDSVQFEQRGHEAFYHVDSVEGAWN
                     RGRAETPNLGYKPGSGLGYFPCPPTDSLVDIRSEMAQRMAECGIATAAHFHEVATGGQ
                     CEIDLVPRPLVESADQVVTAKYIIRNVARRSQKTATFMPKPLYGDNGSGMHTHLTLWK
                     DNQPLLAGQGYAGLSDLGIHAIGGLIKHAAALCAFANPTTNSYKRLVPGFEAPTKISY
                     SRRNRAAIIRIPVNSPSPRSRRIEYRGPDSAANPYLLFSAMLMAVLDGIQNKLRPGEP
                     LDKDIYDLQPEELDTVPTTPRSLEASLEALKADHEFLLRGDVFTPDVIDTWIWYKQSH
                     EVETIRVRPHPYEFSLYYDV"
     gene            complement(1770..2351)
                     /gene="lspA"
                     /locus_tag="VHS97_19765"
     CDS             complement(1770..2351)
                     /gene="lspA"
                     /locus_tag="VHS97_19765"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX3450503.1"
                     /translation="MKPDGSAGIGRLMLFWAIALGGTAFDLTTKSMVFAKVGPPPARP
                     HTIVPHILELHTSHNTGALWGFGSGLPGSSYIFAGLSVVAGVVIIYYLFWLGAASSRV
                     LTVALGLIMAGAMGNCFDRLKFGYVRDFVHFHVDAIGFDCAIFNFADNMLVAGAVTLV
                     LYALRPEKSPGEEPPAGFPLEASSTSSQSVSSA"
     gene            complement(2348..2734)
                     /locus_tag="VHS97_19770"
     CDS             complement(2348..2734)
                     /locus_tag="VHS97_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX3450504.1"
                     /translation="MASALKQDELGSYRQTLQNLRARLRGDLSQMTDEALRRDSAGGT
                     GNLSNVPLHMADLGTDNYDQEFTLGLIENEQGTLELVVEALARMEKGTFGECVECSGA
                     ISKPRLEAIPYARHCIGCARKLEGGE"
     gene            3058..4092
                     /locus_tag="VHS97_19775"
     CDS             3058..4092
                     /locus_tag="VHS97_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3450505.1"
                     /translation="MNTTSSKTIFITGATGLVGSHATEEAIQRGHRVRALVRPTSDTR
                     WLGQWGVETIVGDLEDPQSLRRGVDGADWVFNCAAKVGDWGTLEEFRRLNVAALRLLL
                     DAAVPARLERFVHVSSLGVYEGRDHYGTDETVPPAANSLDAYTRSKTEAEALVIEYRQ
                     KHGLPAAIVRPGFIYGERDRTVLPKLLLNLRRGSFMYFGSGEQVLNCIYVKNLVSGIF
                     LAAENPAAIGELFNLTDGQPVTKKQFVGRVAELAGLKAPTRRLPLGLARILANVVEGG
                     ARLRGAKNPPIINKARFKFLGLHLDYSIEKARRVLGYQPPFTFEQGIGQAMAEHRPAA
                     SQEAAVAAAT"
     gene            4146..>4403
                     /locus_tag="VHS97_19780"
     CDS             4146..>4403
                     /locus_tag="VHS97_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX3450506.1"
                     /translation="MSNIKSKRVAVLVEKFYEDLELWYPVLRLREAGCDVKIVGPKDG
                     ETYLSKHGYPAKAEVAASEVKAYDFDAIVIPGGYSPDHMRRH"
BASE COUNT          923 a         1385 c         1263 g          832 t
ORIGIN      
        1 aatctcgcac cgatccagat tccagatttc caatcacggt ttccagatca aatagtcgac
       61 catcggccgt ggcgcacgat agagaacgag agtcgatatc aagggttttg gttacatcag
      121 acctatcggg ctcggcaata cgccgtgata atggccctac cggtgcggcg tggtgcgatc
      181 ggaagcgcga cgatcctatg ttcaggctgt atccggatga gagataaaag gagagcggct
      241 gcgtgactcc caaagaagtc ttggcacaga tccggcagcg cgaggtgacg acggtcgatc
      301 tacgattcat ggattttccc ggagtatggc agcatttctc gattccggcc gatgcactga
      361 ccgaggagac gttcgaggaa gggatcggct ttgacggctc gagcgtggtc ggttggcgcg
      421 cgatcaacga gtcagacctg ctggtggtcc ctcagcccga gacggccctg atcgatccgt
      481 ttccagcaca accgactttg acgatgatct gtaacatcca cgatccgatc acccatcaag
      541 actatacacg cgacccgcgc aacattgccc gcaaagccac gagctatatg cgaaacacgg
      601 gcgtggccga tcagtgtctg ctggcgcccg agctcgagtt tttcgttttc gacagcgtcc
      661 agttcgaaca gcgcggtcac gaagcctttt atcacgttga ttcggtggaa ggcgcctgga
      721 accggggccg ggccgagacg cccaacctcg gctacaagcc cggatcgggg ctgggttatt
      781 tcccttgtcc gccgaccgac agccttgtcg acatccgctc ggaaatggcc cagcgcatgg
      841 cggaatgcgg tatcgccacg gctgcccact ttcatgaagt cgccaccggc ggccagtgtg
      901 agatcgacct cgttccgcga cccttggtcg aaagtgccga ccaggtagtc accgccaagt
      961 acatcatccg caacgtcgct cggcgcagcc agaagaccgc gacgttcatg cccaagccgc
     1021 tctatggcga caacggctcg ggtatgcaca cccatctgac gctctggaag gacaatcagc
     1081 cgctcctcgc tggccagggg tatgccggtc tgagtgacct gggcatccac gccatcggcg
     1141 ggttgatcaa gcacgcggcg gcgctctgcg cgtttgccaa tcccaccacg aatagctaca
     1201 agcgtttggt gccgggcttc gaggccccga ccaagatctc gtacagccgg cgtaatcgcg
     1261 ccgccatcat tcgcatcccg gtcaacagtc ccagccccag aagccgccgg atcgaatatc
     1321 gcggccccga cagcgccgcc aatccttact tgctcttttc ggcgatgctg atggcggttc
     1381 tggacgggat tcagaataag ctgcggcccg gcgaaccgct cgataaagac atctacgatc
     1441 ttcaacccga agagctcgat accgtaccca ccaccccgcg ctcactggaa gcgtcgctcg
     1501 aggctctgaa ggccgaccac gaatttctcc tccgcggcga cgtctttacc cccgacgtca
     1561 ttgacacgtg gatctggtac aaacagtcgc acgaggtcga gaccatccgg gtccgtcccc
     1621 acccctacga attctccctg tactacgacg tttaaatcgt tgtagcacag gcgacttgcc
     1681 ggtgatgaaa ccacggcctg gaaggtcgtg ccaggatggg cggcgtcacc ttgtcgagac
     1741 gtcggtcttc actaccgact cgcccggcat caagccgagg agacggattg ggatgaagtg
     1801 gaggaggctt cgagaggaaa tccagccggc ggctcctcac ccggagactt ctcgggccgc
     1861 aacgcgtaga gcaccagcgt gaccgctccg gcgaccagca tgttgtcagc gaagttgaag
     1921 atcgcgcaat cgaagccgat ggcatcgacg tggaagtgaa cgaagtcgcg tacgtagcca
     1981 aatttcaagc gatcgaagca attccccatg gctccggcca tgatcaagcc taatgcgacg
     2041 gtcaaaaccc ggctcgacgc tgcccccagc cagaacaggt agtaaatgat cacgacgcca
     2101 gccacgaccg agagacccgc gaaaatatag ctgctccccg gcaagcccga tccaaaaccc
     2161 cagagcgcgc ccgtgttatg actggtgtgc agctcgagaa tgtggggcac aatcgtatgc
     2221 ggtcgagcag ggggggggcc gactttcgcg aataccattg acttggtcgt cagatcgaac
     2281 gcagtcccac ccagggcgat cgcccagaag agcatgagcc gtccgatgcc cgcactcccg
     2341 tccggcttca ttcgcccccc tccagcttcc gtgcacagcc aatgcaatgc cgcgcgtaag
     2401 gtattgcctc gagacgcggc ttggagatcg ccccggaaca ctcaacgcat tcgccgaatg
     2461 tccctttctc catgcgtgcc aaggcctcga cgactagctc gagcgttccc tgctcattct
     2521 cgatcaggcc cagggtaaac tcctggtcgt agttatccgt acccaggtcg gccatgtgga
     2581 gcggaacgtt ggaaagattg cccgttccac ctgcactgtc ccggcgaaga gcttcgtcgg
     2641 tcatctggct cagatcacca cgcagccgcg ctcgcaaatt ctggagcgtc tggcgataag
     2701 accccaactc atcttgtttt agagcactcg ccatgaaacc taccccttcc tctccgcacc
     2761 gatctccacg atatccagaa agggacagga tttggcgaat cctagaccct tgtcgtcttc
     2821 ctgtcaaacc gaacactgcc tcgacttgcc gagagatcgg ccaaggactt tttccccctg
     2881 cctcactcgc gcgcttccga gaacctcaag cctacactgt tcgacaattc gaataccacc
     2941 ccccatctcg gttttgtctg gttccgagct cattcgattt catgtcgggc attcgtgatg
     3001 taggatacct ggaatgtcat cagcgcaaat ccgtctcctc cgaccgcgcg gagcgtcatg
     3061 aacacgacgt catcgaagac cattttcatt accggcgcga ccggcttggt cggcagtcac
     3121 gcgactgaag aggcaatcca gcgcggtcat cgcgtccgcg cgcttgtccg gccgaccagc
     3181 gacacgcgct ggctgggcca gtggggggtg gagaccattg tgggcgacct ggaagacccc
     3241 caatcgttgc gccgaggagt cgacggggcg gactgggtct tcaactgcgc cgccaaagtg
     3301 ggtgactggg gtaccctgga agagttccgc aggctcaatg tcgcggcgtt gcgcttgcta
     3361 ctcgatgccg ccgtgccggc gcggctcgag cgtttcgtgc acgttagttc gctgggagta
     3421 tacgagggcc gcgaccatta cgggaccgac gagaccgtgc cccccgccgc caattctctt
     3481 gacgcgtaca cccgctcgaa aacagaggcc gaggcactcg ttatcgagta ccgtcaaaag
     3541 catggattgc cggcggcgat cgtgcgcccg ggattcatct atggcgagcg cgaccgcacc
     3601 gtcctgccca agctgttact caacttgagg aggggcagct tcatgtactt cgggtcgggc
     3661 gaacaggtgc tcaactgcat ttacgtgaaa aacctagtga gcgggatctt tctcgccgcg
     3721 gagaaccctg ccgccattgg cgaactcttc aacctgaccg acggccagcc cgtgaccaag
     3781 aaacagttcg tgggccgggt cgccgaactg gcgggactca aggctcccac ccggcggctg
     3841 ccgctgggcc tggcgcgcat ccttgccaat gtcgtcgaag gtggcgccag gctccgaggc
     3901 gccaagaatc cccccatcat caacaaggcg cgtttcaagt ttctcggatt gcacctggac
     3961 tactcgatcg aaaaggcacg gcgcgtgctc ggttatcagc ctccgtttac cttcgagcaa
     4021 ggtattgggc aggcgatggc cgagcatcgg cccgccgctt cgcaggaagc cgcagtcgct
     4081 gcggcgacat gagttcccca cggaaaaaga gccccgtaac tggggggaga tacggagaga
     4141 tcatcatgag caacatcaaa agcaaacgcg tggcggtttt ggtcgaaaag ttctacgaag
     4201 acctggagct ctggtatccg gttcttcggc tccgcgaagc ggggtgcgac gtcaagattg
     4261 tcggccccaa ggacggcgag acttatctgt ccaagcacgg ataccctgcc aaagcggaag
     4321 ttgccgccag cgaggtcaag gcgtatgact tcgacgcaat cgtgatcccc ggcggctatt
     4381 cgcccgacca catgcgccgg cac
//
LOCUS       DATAQL010001145         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3018288, whole genome shotgun sequence.
ACCESSION   DATAQL010001145 DATAQL010000000
VERSION     DATAQL010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018288"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..986
                     /locus_tag="VHS97_19785"
     CDS             396..986
                     /locus_tag="VHS97_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450507.1"
                     /translation="MAPLKNSRRLTLLDLMIAVAGLAIGLWIMPYGIIPVAETAFPII
                     TGPYAFSARARWLSILIVEYASPVAVIWSFSVLVLNITPPCPPVRRLFRQPGFVACCA
                     VGMTALITGPLSFAISQKIFIAGLSASMRLQIYLGEALTFRRGEGGFAVASAWLVLWA
                     NGRWRPQPSWADRLGRLLGLFWVLTIPFAWPVARSF"
     gene            1122..1586
                     /locus_tag="VHS97_19790"
     CDS             1122..1586
                     /locus_tag="VHS97_19790"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3450508.1"
                     /translation="MIACYCRVSSVRQKTDSQKPEIQRWLQGNGIDPVTADWFEDKET
                     GGTVNRPAFDRLQKAIFAGRVKMVVVWKLDRISRRQRDGVNLLAEWCECGVRVVAVTQ
                     QIDLGGAVGRMVASVLLGLAEIESEYRQERQIERALIRSRGGRLKFELGEAK"
     gene            1665..1811
                     /locus_tag="VHS97_19795"
     CDS             1665..1811
                     /locus_tag="VHS97_19795"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3450509.1"
                     /translation="MERANIERALARCNGQVYGNDGAAALLGMKPTTLWSRLRSLGIS
                     TERS"
     gene            complement(2168..2695)
                     /locus_tag="VHS97_19800"
     CDS             complement(2168..2695)
                     /locus_tag="VHS97_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450510.1"
                     /translation="MAPIKGTPMQRLLAISIILFFPVAVVADDAKNLLNPVNKAESWR
                     LEQHEGAQATLKIADDAVVFNATKLTGTNWHIQAFQVNLDLKEGKEYTLKLKLTASQP
                     RKVLLVATIDAEDWHEIGLHEDLDVNMEVRSSEFTFRATGIVAKKNRIGFVMGDETGD
                     LIVKEMTLTEKVDNK"
     gene            3051..3383
                     /locus_tag="VHS97_19805"
     CDS             3051..3383
                     /locus_tag="VHS97_19805"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX3450511.1"
                     /translation="MSFDGAEEGSHLGAPDFRVGGRIFATLAMQHLGYGNLMLSPTLQ
                     QTLIAEAPEVFLAIPGGWGRKGCTHIRLADASAQQMLKGLQLAWNLRVEKNGRSRSSS
                     PKRRRGKP"
BASE COUNT          669 a         1049 c         1092 g          690 t
ORIGIN      
        1 tcgctccagc accagtcgat gagaccacga tccttgtcat ccagatgaaa gtagcgcccc
       61 agttgggcgg atgtaggctc gcctgcgtag cggccttagc ggcgctgctg ttcctcggtc
      121 aggaattcga cgggcacccc aggccctttc cgaaaaccgc ctcttcgcct tgatgcccag
      181 tgccaaatag ggtcccgcgt ccaaggcatt cgcggacacg gtaaaatcat cggcttcccg
      241 gagccgatac atggtgacaa aatggctaga ccctagctgg cagagccgtc aacgatggat
      301 cgccggattc cgggttgttg tccgggggcc tgtgcctacg gcggatggga agggccgatc
      361 agcaacggga ctttacaggc atgcgaaacc agtcgatggc accgttaaag aatagccgtc
      421 ggttgaccct gctcgacctc atgatcgcgg tcgcagggct cgcgatcggc ctctggatca
      481 tgccctacgg gattatcccg gttgcggaga cggcgttccc gatcatcacc gggccatacg
      541 ccttctcagc gagggcccgg tggctatcca tcctaatagt cgagtatgca tcccccgttg
      601 ctgtcatttg gtcgttctcc gtcctggtgc tcaacatcac cccgccctgc ccgcccgtcc
      661 gaaggctctt ccgccaaccg ggcttcgtcg cctgctgcgc agtgggtatg acggcgctga
      721 tcaccgggcc gctgtccttc gcgatctccc agaagatctt catcgccggc ttgtcggcct
      781 cgatgcggct ccagatctat ctcggggaag ccctcacctt tcgtaggggg gaaggcgggt
      841 tcgctgtcgc gtcggcttgg ttggtcctgt gggcgaatgg tcgatggcgg ccccagccaa
      901 gttgggctga tcggctcggc cgactcttgg gtctattctg ggtcctgacg atccccttcg
      961 cctggccggt ggcccgttcg ttctaacaac gggcctttcc agggcgtcgg ccatgtcttc
     1021 ggcccgggcc atgccggata gcgtatgacc caactgaccc gataacgggg acgagagccg
     1081 cggtggtcgc gccggggtaa tcgaccctgg gagggatcaa catgatcgcc tgctattgcc
     1141 gcgtctcatc ggtgcgtcag aagaccgaca gccagaagcc cgagatccaa cgctggcttc
     1201 aaggcaatgg aatcgacccg gtcacagccg actggttcga ggacaaggag accgggggga
     1261 cagtgaatcg tccggcgttc gatcgactgc aaaaggccat cttcgccggg agggtcaaga
     1321 tggtcgtggt ctggaagctg gaccgcatct cccgccgaca gcgtgatggc gtgaatctgc
     1381 tcgccgagtg gtgtgagtgc ggggttcgcg tagtggccgt gacccagcag atcgacctgg
     1441 gcggggcagt cggccggatg gtcgcgagcg tgctgctcgg gctggcggag atcgagtcgg
     1501 agtaccggca agagcggcag atcgagcgcg ccttgatccg ctcccgaggt ggaagactga
     1561 agttcgagct tggggaagcc aagtaacggc tgctacagaa gcccgcggcg agccgcctgg
     1621 gggatgaccc gtcgcgtgtg ttgacgctca gggagatcaa gcacatggag cgtgccaaca
     1681 tcgagcgtgc gctggcgcgg tgcaacggcc aggtgtatgg caacgatggc gcggctgccc
     1741 tgctcggaat gaagccgaca acgctttggt ctcgactccg tagcctgggt atctcgacgg
     1801 agcggtcctg aaggccgcgc gtgggaaagc gtccggcccg ggaggcgcgg ctcagcgact
     1861 aggaatgaag cccacgacct tggcgtcgcg gatcaaggcg ctgggaatcc tcccgagtcg
     1921 atgagaccac gatcgccccg aggtgcttcg gtccgacgac accccggtcg attcgattga
     1981 ggtcggacga gatccagtcc agagaacttc caatcgttgg cgcatcattg atgccgaatt
     2041 gcagggatct ctgtttgggc cagtccgtga atcctcagtt ccgacctgcg gacggtcgtc
     2101 tccgccacag ccgaccccgc gccgcaagct gtcgacaagt gatcggagac gattgcgaac
     2161 tcgcttgcta tttgttgtcc actttctccg tgagcgtcat ttccttgacg atcaggtcgc
     2221 cggtctcgtc tcccatcaca aaaccgatcc gatttttctt ggcgacgatt ccggtggctc
     2281 gaaatgtgaa ttcggacgag cggacttcca tgttgacatc gagatcttcg tgcaagccaa
     2341 tctcgtgcca gtcttcggcg tcgatcgtcg cgaccagcag caccttgcga ggctgcgagg
     2401 ccgtcaactt gagcttcagc gtgtactcct tgccctcttt caaatcgaga ttgacctgaa
     2461 atgcctgaat atgccagttg gttcctgtca gcttggtcgc attgaatacg accgcatcat
     2521 cggcgatctt gagcgtcgct tgtgcgcctt cgtgctgttc gagccgccaa gactcagcct
     2581 tgttaaccgg attgagcagg tttttggcgt catccgcgac gaccgccacg ggaaagaaca
     2641 aaatgattga aattgcgagc aggcgctgca taggagtgcc cttgatcggt gccattagag
     2701 tccaggagaa caacccccga ttgtcggcac gacggtttca caagtcaacg gacgccgacg
     2761 gttcccgata actcatcgca tcaatcaaga caccgaatcg ccatcggagc tgaggttttt
     2821 gacaaattac caaatcgcgc tgtctccacg agacacccct atcctgacgg acgatcgccg
     2881 gccacgcgac cgcttgcgcg cgtcggtccg acgacaccga cgcgtcccac agccagagcg
     2941 cccaggaaac cgacagggag caccccaagc tggctcgtgt aagacaacca aggacgaccg
     3001 ttgaagtcgg gggtgatttg gtggatgcgg cagactttcg ggaaatggcg ttgagcttcg
     3061 atggagcgga ggaaggttcc catttgggtg cgcctgactt ccgtgtcggc ggccgtatct
     3121 tcgcgactct cgccatgcag catctgggat acggcaactt gatgctgtca cccacgctgc
     3181 aacaaacgct gatcgccgag gcgccggaag tcttccttgc aatacccggt ggttggggga
     3241 ggaagggatg cacccacatc cggttggccg acgcgagcgc gcagcaaatg ctgaagggcc
     3301 tccaactcgc ctggaacttg cgggtcgaga agaacggtag gtcgaggtcc tcgagcccga
     3361 aacgacgacg cggaaagcca tagcatcgga gaagcctggt tggtcgagcg caaacgcccc
     3421 acagtgtggc ctgcaccaac tggccaacct tggcggctgc ccagcggatt gagcgccgct
     3481 gaatctggct ccgtttccag
//
LOCUS       DATAQL010001146         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1185655, whole genome shotgun sequence.
ACCESSION   DATAQL010001146 DATAQL010000000
VERSION     DATAQL010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185655"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..503)
                     /locus_tag="VHS97_19810"
     CDS             complement(234..503)
                     /locus_tag="VHS97_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450512.1"
                     /translation="MRDRLSVLISQGIDETGWSLRSTDAVTGDESTGYHIVGEGLGPD
                     RHLEAFLVTGLYFALVPEPSSPALGGVAAVAWLGRRAWAAARASG"
     gene            945..1907
                     /gene="fmt"
                     /locus_tag="VHS97_19815"
     CDS             945..1907
                     /gene="fmt"
                     /locus_tag="VHS97_19815"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX3450513.1"
                     /translation="MAPSNRIIMLGTGDFALPTFEHLIETGHNVVALVTQPDRPQGRK
                     QELIPSKIKRSAEGWRVLVEQPEDVNASASLVRISELKPDLLVTAAYGQILSGTLLSI
                     PGLGGINLHGSILPAYRGAAPVARAIQNGETETGVTVIRMTPQIDAGGIVSIARAPID
                     PEETAGELEARLAQLGAPLIVDAIAALVSGAVTILPQDRSKVTKAPKLRKEDGLIDWS
                     RPALSVHNLVRAMQPWPVAYTIWRPRSMPSTEPVRWIVHKTQVVDGQGEPGEVIEAAG
                     DRLVVAAGVGAVALLTIQLAGKKPFTAAEFLRGHRIQPGDRLGL"
     gene            2080..>4049
                     /locus_tag="VHS97_19820"
     CDS             2080..>4049
                     /locus_tag="VHS97_19820"
                     /inference="COORDINATES: protein motif:HMM:NF024764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450514.1"
                     /translation="MRLQRVVLGIGLTIVLGVTIDGMAQARGTNDSRLTGLKLYASKK
                     YQEAIRYFDQVLASHKRDREILIKRVACYLVTNQPEKALADFDRVNEYSGWASRVFGA
                     GVDGFGGFAPGSTWIGMPTPDVTFAENWGNRGLALLMLERNEEALQSFRTSTDLWSLP
                     QNQSMIRGRAGAYQGLGQAYHRLGQDELAFQTYSAAISVDATDPNGFAGRGDVLASLK
                     LLGAAEADFNEAIRLDPTHSRAYCGRGIVRSDLGRVEPALADLDRAIALDPKYAKAYS
                     HRGGLHARRGQNELALADYDALIQVLPDNAGAYKDRGGVLVRMNRFDRAVDDLSEAIR
                     LAPKSAAAYQNRGAAYNGLGQYERAIEDLSKAIELKHDNAGAYTNRGLAYFAVGQFDQ
                     AVADLSEAITIAPRNAIPYFNRAEVFARLGLRDRAIQDYDATVQLDPRLTAAYAASAR
                     LREDKGQRDHAIHDFDMALRLDPNEVNLLYDRANTRRETGDWRGALADYDRAVVLAPQ
                     KAETYVARGWSRFCAGVEGADLDARVYLKLKGWRDSFSPYMAILAALAARQAQQPEEA
                     KRVLDEAVANLSPRLWPVPVLRYLQGELTEPALLDLAVSTRQQAEAHTFIGLDRLRSG
                     DRAGAILHLHWARDHGSEGSIALDVAQGTLARL"
BASE COUNT          800 a         1298 c         1232 g          719 t
ORIGIN      
        1 cagtcgatga cgaattaaat catgttgata ttgttgttaa atgcgtacct tcttcggtcg
       61 caaggtccgt gcccccgtca agtaccacca agctttctgc ttcatccttc gcacggtcgg
      121 ccagggcatc ggcagcgtgc gagcgggaga ggctactagg tagaccatta cgatgcgagg
      181 cctcggagag tttgccggtg cagcgtcggt ttcatcgacc ggtccggtgg acgtcagccc
      241 gaggcccgag cggccgccca cgcccgccgc cccagccacg cgaccgccgc gacgccgccc
      301 agggccggcg acgacggctc ggggaccaat gcgaaataca acccggtcac gaggaaagcc
      361 tcgaggtggc gatcaggccc caacccttcg ccgacgatgt gatacccggt cgactcatcg
      421 ccggtgacgg catcggtcga ccggagcgac cagcccgtct catcgatccc ctggctgatc
      481 aacaccgaga gccggtcgcg cacttgcttt ccacggacac ggcgaaatac accgcgacgg
      541 tcccggcggc gttgcgacga gccccgtgat ctcggcctgt tgcggcccgc atcgaagatt
      601 gcacgaaggc agcgaaggtt gcacacggaa ttggactgca atcttcgcta ccttcgaacg
      661 atccgtgcga tcttcggtcc cggcgcggcc gcaacgttcc gctcaccgcc ggctgcgcac
      721 ccagcccggc gcttgaatcc tcacccgcgg caccaagcaa accggcgccg accacgtgtt
      781 ggcccttgca gcagcggtgg gcacgctaag aaaatagccg taacgcttcc ggccagagtc
      841 gcgttgccat ttcctggcat ttggaggctt tggtgcaaac gagcaacccg cagtccgaat
      901 tatgcgagac ttcctttaac cgagccgcca agtagctctt taccatggcc ccttcgaatc
      961 gaatcatcat gctaggcaca ggcgactttg ccctgcctac cttcgagcac ctgattgaaa
     1021 ccgggcacaa cgtcgtcgcg ctcgttaccc agcccgaccg accccagggt cggaagcaag
     1081 agctgatccc cagcaagatt aaacgctcgg cagaagggtg gcgggtcttg gtcgaacagc
     1141 ccgaggacgt gaatgcgtca gccagcctgg ttcgcatcag cgaactgaag cccgacctac
     1201 ttgtcacggc ggcgtatggc cagatcctct cgggaacgct tttgtcgatt cccgggctcg
     1261 gtgggattaa cctccacgga tcgatcctgc cggcatatcg aggcgcggcc ccggtggcgc
     1321 gcgccattca aaacggcgaa acggagaccg gcgttaccgt catccgcatg acaccccaga
     1381 tcgacgcggg cgggatcgtg tcgattgcac gcgccccgat cgatccggag gaaacggccg
     1441 gcgagctcga ggcacgtttg gcccagctcg gtgccccatt gattgtggat gccatcgccg
     1501 cccttgtgtc tggtgcggtc acaatcctgc ctcaggatcg atcgaaggtg accaaagcac
     1561 caaagcttcg caaggaagac ggtctgatcg attggtcacg gccagcattg tccgttcata
     1621 acctggtgcg cgcaatgcaa ccgtggcccg tggcctacac catctggcgg cctcgatcga
     1681 tgccgtcgac cgagccggtc cgatggattg tgcacaagac ccaggtggtt gacggccagg
     1741 gagagcccgg ggaagtgatc gaggccgcgg gcgatcgcct ggttgtcgcc gcaggcgtag
     1801 gcgccgtcgc actcttgacg atccagctcg ccggcaaaaa gccgttcacc gccgccgagt
     1861 tccttcgcgg ccatcgcatc caacccggtg atcggctggg cctttaatca agcgagaacg
     1921 cccaagcgag aaccgccggc caccccttca aagctcagtc gccatacaac cctcaactcc
     1981 gtcgcgcgca aatcgctgac cggtcgatcg ccgcctcctg ctggagacga caatgagaga
     2041 gcaggagctc tccccggagt gcgaaggcga ggatggacca tgcgacttca gagggttgtg
     2101 ctgggaatcg gtttgacgat cgttctgggc gtgacgattg atgggatggc ccaggcacgc
     2161 ggcacaaatg atagcaggct cacggggctg aaactgtatg catcaaagaa gtaccaggag
     2221 gcgatccggt acttcgacca ggtattggca agtcacaagc gcgatcgaga gattttgatc
     2281 aagcgcgtcg cttgttatct ggtcacgaac cagcccgaga aggcactcgc cgattttgac
     2341 cgagtgaatg agtatagcgg ctgggcatcg cgggtttttg gcgccggcgt cgatgggttc
     2401 gggggctttg cgcccgggag cacctggatt ggcatgccta cacccgacgt gacgttcgcg
     2461 gagaactggg gaaaccgagg cctggccctc ttgatgctgg agcggaacga ggaagcgctc
     2521 caaagctttc ggacgtcgac tgatctctgg agtctgcctc aaaaccagtc gatgatccgc
     2581 ggccgcgccg gagcctatca agggcttggc caggcttatc acagactcgg tcaagacgag
     2641 ctggcctttc aaacgtacag cgcagcgata tccgtcgacg caaccgaccc caatggcttt
     2701 gccggacggg gcgatgtact ggccagtctc aagctcttgg gcgccgccga agccgacttt
     2761 aacgaagcaa tccgactcga cccgactcat tcacgggcct attgcgggcg cggcatcgtt
     2821 cgatccgatc tcggccgtgt cgaacccgcg ctcgccgacc tcgaccgcgc catcgctctc
     2881 gatccgaaat acgccaaggc ctattcccat cgaggcggtc tgcacgcacg acggggccaa
     2941 aacgagctgg cgctggccga ctacgatgcg ctcatccagg tcctcccgga caacgcgggc
     3001 gcctataaag atcggggtgg agttctcgtg cggatgaacc ggtttgatcg cgccgtcgac
     3061 gacctgagcg aagcgatccg gctcgcgccc aagagtgctg cagcctacca gaaccgcggt
     3121 gccgcctaca acggattggg ccagtatgaa cgcgcgatcg aagaccttag caaggcaatc
     3181 gagctcaagc acgacaacgc cggcgcgtat accaaccgcg gactggcgta tttcgccgtc
     3241 gggcaattcg accaggcggt cgccgatctg agcgaagcga tcacgatcgc gcccagaaac
     3301 gccattccgt atttcaaccg agccgaggta ttcgcacggc ttgggctgcg cgaccgtgca
     3361 attcaagatt atgacgcgac ggtccagctc gatccgcgcc ttaccgcagc ctacgccgcc
     3421 agcgcgcgtc tccgtgaaga caagggtcag cgcgatcacg cgattcacga tttcgacatg
     3481 gcccttcggc tcgaccccaa tgaggtcaat ctgttgtatg accgagccaa tacgcggcgc
     3541 gagaccggtg actggcgtgg tgcattggcc gactacgatc gcgcggtggt tctcgcgccg
     3601 caaaaagccg aaacgtacgt cgcgcggggt tggtctcggt tctgtgcggg ggtcgaaggc
     3661 gccgatctcg acgcgcgtgt ttacttgaag ctcaagggct ggcgggacag cttttcgccc
     3721 tacatggcga ttctcgccgc gctggcggcg cgccaggcac agcagcctga ggaggccaag
     3781 cgagtccttg acgaagccgt cgccaacctc tctcctcgcc tctggcccgt ccctgtcctg
     3841 cgatatttgc agggtgagct cactgaacca gccctgctgg atcttgccgt cagtacacgg
     3901 caacaagccg aagcacatac gtttataggg ctcgaccgcc tgcgctcagg cgaccgcgcc
     3961 ggcgcgattc ttcacctgca ttgggcgaga gaccacggca gcgagggctc gatcgccctc
     4021 gacgtcgctc agggaacgct tgcccggct
//
LOCUS       DATAQL010001147         3899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3018473, whole genome shotgun sequence.
ACCESSION   DATAQL010001147 DATAQL010000000
VERSION     DATAQL010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018473"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1183)
                     /locus_tag="VHS97_19825"
     CDS             complement(86..1183)
                     /locus_tag="VHS97_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450515.1"
                     /translation="MPRIELRKLAIALVALVATASIARGAEPTASLKKGTPDLKSAGP
                     LAFGPDGILLVGDTKGAALFAIDTGERKAAAGKRAIEVKDLAGKVAGMLGTDAKQIAI
                     NDMAVNPQSGTVYLSVSRGRGPDAIPLLVRINADGKAEEFSLENVHFAKADLPNAPAN
                     ERQRVESITDVAFADGRVFVAGLSNEEFSSRLIAIPFPFSSEGFDGASIEIYHGAHGK
                     LETKSPIRTFVTYRIQNEPYMLAAYTCTPLVKLPVSELKAGAHIKGTTIAELGNRNRP
                     LDMIVYHKDGKDYLLMNNSSRGVMKIPTENAGSAEGVIAPVNGKKGIGYDTIDSLKGV
                     AQMDQLDDHHAVLLVQEDPNGSMDLRTIELP"
     gene            1475..3445
                     /locus_tag="VHS97_19830"
     CDS             1475..3445
                     /locus_tag="VHS97_19830"
                     /inference="COORDINATES: protein motif:HMM:NF019227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HEX3450516.1"
                     /translation="MVNTLVVCCALLGAGPGESATKTLPGPADLAAYAAAKSKLGRDA
                     DAHVRLAVWCESHGLEAERMKHLAMVILYDPSHALARGLLGMVAYQGKWSRAEIAGKQ
                     IENDPAYQDAIREYLDRRARTPDKADSQLKLAAWCEQKGLKAQALTHYEEVIRLDPAR
                     DAAWRHLGYKKQGTRWIKPDLAAADKQEAERQKQADKYWKPILTKLRDDLQSKDQARR
                     SRAEQELSQVSDPRAVPTIWALFIAGSERSQLAAIEMLGQIDGPAASSTLAALAIFCP
                     RTEVRNRAFDRLLSRDLREIMTRLIGLIKKPFKYQVRPVNGPGSTGVLFVEGEKFNVE
                     RVYRSMPVDPSLFPQVAANSRPNTPLMVENQLGMPANAGGDPFDYLAAQVASAASSSR
                     ELRDGINRLQQVRQANQAVAQTLAQDVQTIEAINDQINTLNARLLPIVESASGQKLGA
                     EPEKWKSWWTDKLGYAFQASQPTTKPTFTDLVDASSWSASLECFAEGTLVHATGGPKA
                     IETIRVGDRVLSQDPTTGALTYQPVMAVHRNQNAATVRIGVDGETIVATGIHRFWKAG
                     KGWTMARDLKAGDRLRMLGSIALVKSVDKDASQTVYNLDVALNRDFFVGSQGLLVHDS
                     NFVKPEAEPFDQEPDLATLSTNAAPVSNARTP"
     gene            complement(3524..>3899)
                     /locus_tag="VHS97_19835"
     CDS             complement(3524..>3899)
                     /locus_tag="VHS97_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX3450517.1"
                     /translation="GLESHPTLLVLNKSDKVPDRSYLDVLKAHHSGSVAISAACGDGL
                     DRLEAAVRSALSQRDLEAEIETGVSNGRVLAYLAQHAQIHNRTYDADRVRLHCHLPKR
                     CLEFLFEHGVDVRTESQRLWAS"
BASE COUNT          773 a         1172 c         1186 g          768 t
ORIGIN      
        1 gcggtctgac cggccggagc gttcccttcc gcgagggaag acgccccggc tagcacaatc
       61 aaagtggtga gacaacaacg gaagatcacg gcaattcgat cgtccggaga tccatcgagc
      121 cattgggatc ttcttgcacg agcaagacgg cgtggtggtc gtcgagctgg tccatctgcg
      181 ccactccctt gagactgtcg atggtgtcgt aaccgattcc cttcttgcca ttcaccggcg
      241 cgatgactcc ttcggccgat ccggcgttct cggtggggat cttcatgacg ccccggctgc
      301 tattgttcat caagagataa tccttgccat ccttgtggta gacgatcatg tcgagcggcc
      361 ggttgcggtt gcccagctcc gcaattgtgg tccctttgat atgggcgcca gccttgagct
      421 cggagacagg cagttttacg agcggcgtac aggtgtatgc cgccagcata taaggctcgt
      481 tttgaatccg gtatgtaacg aaggtgcgga tcggcgattt ggtctcgagc tttccgtgtg
      541 cgccgtggta gatctcaatg gaggctccgt cgaagccctc gctgctgaat gggaacggga
      601 tggcgatcaa ccgcgacgaa aactcttcgt ttgacagccc ggcgacgaag acgcggccgt
      661 ccgcgaatgc cacgtcggtg atcgactcga cccgttgccg ctcattcgca ggagcgttgg
      721 ggaggtcggc cttggcgaag tggacattct cgagcgaaaa ctcttcggcc ttgccgtccg
      781 cgttgatccg aaccaagagc gggattgcgt cgggcccgcg tccgcgcgac accgacaaat
      841 agacggttcc cgactgggga ttgacggcca tgtcgttgat cgcgatctgc ttggcatcgg
      901 ttccgagcat gccggcgacc ttgccggcca gatccttgac ttcgatggcg cgcttcccgg
      961 cagccgcttt ccgttcgccg gtatcaatcg caaacagcgc tgcgcccttg gtatcgccca
     1021 ccaacaggat tccatccggc ccaaaagcca gcggcccggc cgacttcagg tcgggcgtcc
     1081 cttttttcag gctcgccgtg ggctctgcgc cccgcgcgat cgaagcggtc gcgaccaaag
     1141 ccacgagcgc gattgccagc ttgcgtaact caattcgcgg catctttcgg ttctccttca
     1201 gtgttgaacc cgtcggtttg cacctatgcc taacgatgtc tctcgaactc gcattatcgc
     1261 cgcactcgcg gagtaatcaa cgagtcaggc aggattctgc ccctgaattt tttcggacgg
     1321 tgtaattccg gactcggttc ttgcgtccaa ggcgcctact tcattaagat ccagacctta
     1381 cgcagacatc gatgtgtgtc ggtcgctcca accacttctt cgcaacttct cgcttggcct
     1441 gagttcggct gctctactta ctggaggcgg gatcatggtc aacactctgg tcgtgtgttg
     1501 tgcgttgctc ggcgcgggtc cgggtgagtc agcaacgaag accttgccgg gcccggccga
     1561 tctcgcggcc tacgcggcgg ccaagtccaa actagggcgc gacgccgacg cgcacgttcg
     1621 actcgctgtc tggtgcgagt cgcacggcct ggaggccgag cgaatgaaac atctggcgat
     1681 ggtgatcttg tatgacccat cccacgccct ggcgcgagga ctgttgggga tggtcgccta
     1741 tcaagggaag tggagccgcg ccgagatcgc ggggaagcag atcgagaacg atccggccta
     1801 ccaggacgct atccgcgaat acctcgaccg tcgcgcccgc acacccgata aggcagattc
     1861 gcagctcaag ctggcggcgt ggtgtgaaca aaaaggcctg aaggcgcagg ctctgacgca
     1921 ttacgaagag gtcatccgac tcgatccggc acgtgatgcc gcgtggcggc acttgggcta
     1981 caagaaacag ggaacgcgct ggatcaagcc cgatctagcg gcggccgaca agcaagaagc
     2041 cgaacggcaa aagcaagccg acaaatactg gaagccgatt ctgacgaagc tacgcgacga
     2101 cctgcaaagc aaggatcaag cgcggcggtc gcgggccgag caagagcttt cccaagtatc
     2161 cgacccacgc gccgtcccaa cgatctgggc gctctttatc gccggttcgg agcgatcgca
     2221 gcttgcggcg atcgagatgt tgggccagat tgatgggccc gcggcctcga gtacgctcgc
     2281 tgcattagcg atcttctgtc cgcggaccga agtgcggaac cgcgcttttg accgcctcct
     2341 aagccgtgat ctgcgcgaga tcatgacccg gctaattggg ctgatcaaaa agccgttcaa
     2401 ataccaagta cgcccggtta atgggcccgg ttcgacgggt gttctgtttg tcgaaggtga
     2461 gaagttcaac gtcgagcgag tctatcgatc aatgccggtt gacccgtctt tgtttccgca
     2521 ggtcgccgcc aattcgcggc cgaacacccc gctcatggtc gaaaaccagc tgggcatgcc
     2581 ggcaaacgca ggtggcgatc cttttgacta tctggccgcg caggtggcgt ccgccgcttc
     2641 ctcatcgcgc gagttacgag atgggatcaa tcggttacag caagtccgcc aggcaaacca
     2701 ggcggtcgct caaactctgg cgcaagacgt ccagacaatc gaagcgatca atgatcagat
     2761 caacacactc aacgcccgcc tgctgcctat cgtcgagtcg gcgagcgggc agaaattggg
     2821 tgccgagccc gagaagtgga agagctggtg gacggacaag cttggctacg cctttcaggc
     2881 cagccagccc acaaccaagc cgactttcac cgacttggtc gatgcgtcga gctggtcggc
     2941 ctcgctggag tgcttcgccg aagggacttt ggtccacgcc acaggtggcc ccaaggcaat
     3001 cgagacgatc cgcgtgggtg atcgcgtgct gagtcaagat ccgacgacgg gcgccctcac
     3061 ctaccaaccg gtcatggccg tccatcgcaa ccagaacgcg gcgaccgtcc ggatcggcgt
     3121 cgacggcgag acaatcgtgg cgacaggcat tcatcggttc tggaaagccg gcaaggggtg
     3181 gacgatggcg cgtgatctga aagccggtga tcggcttcgg atgctcggca gcatcgccct
     3241 ggtcaaatcg gtcgacaaag acgcgagtca gaccgtttac aacctcgacg tcgccctcaa
     3301 ccgcgatttc ttcgtgggga gccaggggct cttggtacac gactccaact tcgtgaaacc
     3361 cgaagccgaa ccgtttgacc aagagccaga cctcgccaca ctctcgacaa atgcggcgcc
     3421 cgtctcaaat gcccgcacgc cgtgagacct gtgcatgagc cggcctcctc atgttgttag
     3481 cgatctgggc ttcagttccg attgattccg aatgcggctt caatcacgaa gcccagagcc
     3541 gctggctctc cgtacgaaca tcgacgccgt gctcaaagag aaactccagg cagcgcttgg
     3601 gaagatggca gtggagccgc acacggtcag cgtcatatgt gcggttgtga atttgggcgt
     3661 gctgtgcaag gtacgccagc acgcggccgt tggaaacacc tgtttcgatt tccgcctcaa
     3721 gatcgcgctg gctgagcgct gagcgcacgg cggcctcgag gcggtcgagt ccatcaccgc
     3781 acgcggcgct gattgcgacg gatccagagt ggtgagcctt gagcacgtcg agataagatc
     3841 ggtcgggaac tttgtccgac ttgttcagga ccaagagcgt ggggtggctt tcgaggccc
//
LOCUS       DATAQL010001148         5346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_116354, whole genome shotgun sequence.
ACCESSION   DATAQL010001148 DATAQL010000000
VERSION     DATAQL010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116354"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /gene="thiS"
                     /locus_tag="VHS97_19840"
     CDS             complement(<1..284)
                     /gene="thiS"
                     /locus_tag="VHS97_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX3450518.1"
                     /translation="MEPVVQITLNGECREIPGLLSVAELLRQLGLKPEHVAVEVNRDL
                     VTRSRQSETVIANGDVLEVVTLVGGGGAATIPIEPLFIGRHVVRSRLFVGT"
     gene            569..1027
                     /locus_tag="VHS97_19845"
     CDS             569..1027
                     /locus_tag="VHS97_19845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX3450519.1"
                     /translation="MFVLQITTAVGLSTLLAAAVFSQGTSSKPEKSCTPESLVGRYDI
                     VDGEKEGTKEPAERIKGTTVTVTKDSIVVADKEKKEIYASFYKLDSTTNPCSITMTSR
                     VESSAGEIARGLIQKDGDTVRLIYALPTGEIPAGFKTKEKQLMFVMKKTG"
     gene            complement(1092..1643)
                     /locus_tag="VHS97_19850"
     CDS             complement(1092..1643)
                     /locus_tag="VHS97_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX3450520.1"
                     /translation="MAMIATEANGSRPVPLPGRVLGIDPGLNVTGYAVVEPSHRGPFV
                     AEAGVIRPRSAQKVMGARLYYIHCAIIEVLEAFPPSSVALERIHSHVRYPRTAILMAH
                     ARGAIMLAAAQRRTPVFGYAATRIKKTLTGSGRAPKTQIQHAIMTELGLDELPEPHDV
                     ADACAVALCHFHVSRNQRGLNEG"
     gene            complement(1677..3170)
                     /gene="cysS"
                     /locus_tag="VHS97_19855"
     CDS             complement(1677..3170)
                     /gene="cysS"
                     /locus_tag="VHS97_19855"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX3450521.1"
                     /translation="MALRVYNTLTQTKEPFETVHPGKVGMYVCGPTVYSESHIGHMVG
                     PVIFDTIKRYLVYLGYEATWVVNITDVDDKLIVQAQKDGTTVGELAERVTRDYLECLA
                     ALGVDGIDHMPRATEHIGDIIAINQSLIDKGFAYESGGDVYFDVGKAAEYGKLSHRDP
                     EELLAGARIEPSVLKHHPGDFALWKRSKPGEPSWQSPWGPGRPGWHIECSAMSMKYLG
                     RHFDIHGGGLDLVFPHHENELVQSESYSGETFATYWMHNGLLTKQGKKISKSDPGTIV
                     LMSDLLKVHSADTLRALLLSSHYRRPIDFGPERLDEIDRGLQTFYRAFERFTELTGER
                     FDQLVAPTRRAPLPFDAGSSPLLKEIAEHRDQFLDAMDDDFNTGGAFGTLFEIVHALN
                     RFSNQIKPDPSSSANGTAADYLRGMVVLKELSQILGLFHRPVAAGQPVADALTAPLLS
                     LLIDLRARLRKEKNFGLADEIRNRLTELGVTLEDRPDSTRWRIESRR"
     gene            complement(3262..3744)
                     /gene="ispF"
                     /locus_tag="VHS97_19860"
     CDS             complement(3262..3744)
                     /gene="ispF"
                     /locus_tag="VHS97_19860"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX3450522.1"
                     /translation="MRVGIGHDTHRLVEGRRLVLGGVSIDHPLGLFGHSDADVVCHAV
                     ADALMGAAGLGDIGELFPDTDPQWRGLDSTRLLAEVVDRIEQTGWRAVNCDVIIHAQE
                     PKLGVHKQSIRANLSRLLRVELSCVNVKAKTGEHVGPIGRGEAISCHAVVLLESSTKG
                     "
     gene            4032..4370
                     /locus_tag="VHS97_19865"
     CDS             4032..4370
                     /locus_tag="VHS97_19865"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEX3450523.1"
                     /translation="MQYGNTEQRGPDKDVPDRLDSGIFLECKGARQFEVRSRIDIPDR
                     KKEDAMLVLSRKLNEKIVIDGGIVVTVVKIDRNQIRLGIEAPGNIRVFREEIVGNARR
                     GADQPVGASA"
     gene            4561..>5346
                     /locus_tag="VHS97_19870"
     CDS             4561..>5346
                     /locus_tag="VHS97_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3450524.1"
                     /translation="MPDESDLTGTINRRDFLGTGAGAIAVASTTVGIAPEAGAQTGTQ
                     ASKSTVLPKRTLGRTGVEVSMLNLGTWKSVGLDRVLRFAWANGIRYVDTAKSYESEPA
                     IGRWLQAMPEVRKQLFLVTKDHPRTPRELIGQLDERLDALKTDYVDLIFIHAVGDHDF
                     ATEWKWPKSREFKETAEAIRKSGKARFVGFSTHHKNRAAILQAAADGGFVDVIMLQNN
                     PWIAEELGQKDQINRALDNCHKAGIGLISMKQVAGNTKLDEIGR"
BASE COUNT         1168 a         1554 c         1582 g         1042 t
ORIGIN      
        1 gtcccgacga acagccggct ccggacgacg tgcctgccga taaaaagtgg ttcgataggg
       61 atcgtcgcgg cgcctccacc gccgaccagc gtcacgacct ccaagacgtc tccattggcg
      121 atcaccgtct cggattgcct ggaccgggtg accaggtctc tgttgacctc gaccgccacg
      181 tgctcgggct tgagacccag ctgtctgagc agctccgcca ccgagagcag ccctgggatc
      241 tcacgacatt caccgtttag agtgatttga acaaccggtt ccacggatat gctccggcca
      301 actccagcac agactgtaag ccccgttcca tggtcggaaa cccggtgctc gacaatcggc
      361 cgccatgtcg tcctaagttc aaagggcagt ttcagatccg ccctggagat tattccctta
      421 gcccaggtca tcgtatcgcc gccggcgcag gcgggtcaag ccgccgagat ttggacgtgc
      481 ggattctaga gatcggcacc gcagttgcgt tctcgttctc catcgtaaca aaaagacaac
      541 ttgtcactcc gctggatgga gaacacccat gttcgtactc caaatcacga ccgcggtggg
      601 attgtcgacg ttgctcgcgg cggcagtgtt ttctcaaggc acgtcatcca agcctgaaaa
      661 gtcgtgcact ccagaatctc tggtaggccg atatgacatc gtggatggcg aaaaggaagg
      721 gacgaaggaa cctgcggagc gaatcaaggg aacgacagtc acggtcacaa aggacagtat
      781 cgtcgtcgca gacaaggaaa agaaggaaat atacgcctcc ttttacaagc tcgactcgac
      841 cacgaaccca tgcagcatca ccatgaccag tcgcgtcgaa tcgtcggccg gcgagatcgc
      901 acgaggactt atccagaagg atggggatac ggtccggctc atctacgcac taccgaccgg
      961 agaaattcct gccggattca agaccaagga aaaacaattg atgtttgtca tgaagaaaac
     1021 ggggtgacgg caaggcgccc gatcgtgtta cggaacggtt tctgatcatg atgtccaccg
     1081 gccgcgtgaa tctaaccttc attcagccct cgctgattgc ggctgacgtg aaagtggcac
     1141 agcgcgacgg cgcaggcatc ggcaacgtcg tggggctcgg gaagctcgtc caagccgagc
     1201 tcggtcatga tcgcgtgttg gatttgggtc ttgggagccc ggccgctgcc ggtcagggtt
     1261 ttcttgattc gggtagcggc gtagccgaat actggcgtcc gccgttgtgc cgcggcgagc
     1321 atgatcgccc cccgtgcgtg ggccatcaag atcgcggtgc gcgggtagcg cacgtggctg
     1381 tggatccttt ccagggcaac tgagctgggt ggaaaggcct ccagcacttc gatgatcgcg
     1441 cagtggatgt aataaagtcg cgcgcccatc actttttgcg ccgaacgcgg acggatcacg
     1501 cccgcctcgg ccacgaaagg cccgcggtgc gacggctcga ccacggcata tcccgtcacg
     1561 ttcaaaccgg ggtctatccc cagcacgcgg ccaggaagag gaaccggccg cgatccgttc
     1621 gcctccgttg cgatcatggc catcctgggc cctcctactt gtcattgctc ctcgagctaa
     1681 cgacgtgact cgatccgcca gcgcgtgctg tcggggcggt cctccaaggt gacgccgagc
     1741 tcggtcaacc ggttgcggat ttcgtcggcc aagccgaagt ttttctcctt gcggagtcgg
     1801 gcgcggagat cgatcaagag actcagcaag ggagccgtca gcgcgtctgc cacaggttgg
     1861 ccggcggcga cgggccgatg gaaaagccca aggatctggc tcaactcttt gaggaccacc
     1921 atgccgcgaa ggtaatcggc tgccgtgccg ttcgcggacg acgacggatc gggctttatc
     1981 tgattgctaa atcgattcag ggcgtgcaca atctcgaaca gcgtgccgaa ggcaccgccg
     2041 gtattgaagt catcgtccat tgcgtccagg aattggtcgc gatgttcggc gatctccttg
     2101 agcaacggcg agctgccggc gtcgaaaggt aagggtgcgc gccgggtcgg cgcgacgagc
     2161 tggtcgaacc gctcgccggt cagctcggta aaccgctcga acgcccggta aaatgtctgc
     2221 aggccacgat cgatctcatc gagccgctcg ggcccgaaat caatcggccg gcgatagtgg
     2281 ctggacaaga gcaaggcacg caacgtgtcg gcggaatgga ccttgagcag gtcgctcatg
     2341 agcacgatcg tgccggggtc ggatttggag atcttcttcc cctgcttggt caaaaggcca
     2401 ttgtgcatcc agtacgtggc gaacgtttct cccgagtaag actctgattg aaccaactcg
     2461 ttttcatggt gcggaaatac caagtcgagg ccgccgccat gaatatcgaa atggcggccc
     2521 agatacttca tgctcatggc cgagcattcg atgtgccagc cggggcgccc gggtccccac
     2581 gggctctgcc acgacggctc acctggcttt gaacgtttcc agagagcaaa gtcgccggga
     2641 tgatgcttga ggaccgacgg ctcgattcgc gctccagcca gtaattcttc agggtcgcgg
     2701 tggctcagct tgccgtactc ggctgccttg cccacatcga agtagacatc gccccccgat
     2761 tcgtatgcga atcccttatc gatcaaggac tggttgatcg cgatgatgtc gccgatgtgc
     2821 tcggtcgcgc gcggcatgtg gtcgatcccg tcgactccga gcgccgccag gcactcgagg
     2881 taatcgcgtg tgacccgttc cgccagttcg ccgacagtcg ttccgtcttt ctgagcctgc
     2941 acaatcaact tgtcgtcaac gtcggtgata ttgaccaccc aagtcgcctc gtagccgaga
     3001 tagaccagat agcgcttgat cgtgtcgaag atcaccggcc cgaccatgtg gccgatgtgg
     3061 ctctcggaat aaaccgtcgg accgcaaacg tacatgccga ccttgccggg atggactgtc
     3121 tcaaacggtt ccttggtctg agtcagagtg ttgtaaacgc gcagcgccat gaatggatac
     3181 caatttgctc ggtccggctc gtgatccttt tcccgatccc ggacacatga atggagatct
     3241 acgttcctgg cgaccgtggg atcaaccctt ggtcgacgac tcgagaagca cgaccgcgtg
     3301 acacgagatg gcctcgcccc ggccgatcgg cccaacgtgt tcgccggtct tggccttgac
     3361 gtttacacac gatagctcaa cgcgaagcag gcgggacaaa ttggcccgga tcgactgttt
     3421 gtgaacaccg agtttgggct cctgggcatg aatgatgaca tcacagttga ctgcccgcca
     3481 tcccgtctgc tcgatccgat cgacaacttc cgccagcaac cgcgtgctat ccagtccgcg
     3541 ccattgggga tccgtatcag ggaagagctc gccgatgtcg ccgagtccgg ccgctcccat
     3601 gagcgcatcg gccacggcgt ggcaaaccac gtccgcatcg gagtgaccga agagtccgag
     3661 cggatgatcg atcgagactc cacccagaac taaccgccgc ccctcgacca gccgatgcgt
     3721 gtcgtgcccg atcccaaccc gcatgtccag gctccgtctt ccgcgaggga ccgattcacg
     3781 ctcaccaccc ttacaaacgt ccatcagatc gtagcgtttt agcggctgcc tggctatccc
     3841 gacacgcgat ctagggcttt gttcccattg gcaggcgcaa ttctgtcatt ttgagaagaa
     3901 aagtttcaag aacggccaca agatcacacg cataccctgg tgaaactaaa tccagttcca
     3961 aaaattaaat cgggtttcag taacaagtta gaacaattca gctcggactt tagttacgac
     4021 tggcactggg aatgcagtat ggtaacaccg aacaacgcgg cccagacaag gatgtgccgg
     4081 atcggcttga ttcgggaata ttcttggagt gcaagggagc gcggcagttt gaggttagat
     4141 cgcgaatcga cattccagat cgcaagaagg aggatgccat gctggtattg agccgcaagc
     4201 tgaacgagaa gatcgtgatc gacggtggaa tcgtggtcac ggtggtcaag atcgaccgca
     4261 accaaattcg gttggggatc gaggcaccgg gcaacattcg ggtattccgc gaagagatag
     4321 tcggcaacgc gcggcgtggt gcggatcagc cggtcggggc aagcgcttag acgtgcgaga
     4381 acacttgccg aacaacggaa gcggcaaaag gagtttcaag aaaggttcaa gaaaagattg
     4441 acataagctg gctatccgct cgacaacata ggcgaggtcg attagcttga gttgcgtcga
     4501 ttttaatgat ttagtgagcc tggagacgtc gcctcaatct gttgcgagtg gagaaaaccc
     4561 atgcccgacg agtcggacct gaccggaacg atcaatcgcc gcgatttcct gggcacagga
     4621 gcaggtgcta tcgccgtcgc atcgacgacg gtcggcatag cgcctgaagc gggtgcacag
     4681 accggaaccc aggcctccaa gtcaacggtg ttgcccaagc gtactctcgg ccgcactggt
     4741 gtcgaggtga gcatgctcaa tctcggcacg tggaagagcg tagggctcga tcgagttctg
     4801 cgctttgcgt gggccaatgg catccgatat gtcgatacgg ccaaaagtta cgaatcggag
     4861 ccggccattg ggcgctggct gcaggcgatg cccgaggttc gcaagcaact gttcctcgtc
     4921 accaaggacc atccccggac gccaagggaa cttatcgggc aactggacga acgcctcgat
     4981 gcactaaaga ctgattatgt cgatctcatc ttcatccacg ctgtaggcga ccacgacttc
     5041 gcgaccgaat ggaaatggcc caagagccga gagttcaagg agaccgccga ggcgatccgc
     5101 aagtcgggca aggcccggtt cgtggggttc tccacccacc acaaaaaccg agccgccatc
     5161 ctccaggcag cggccgacgg tggtttcgtc gacgtgatca tgctgcagaa caacccgtgg
     5221 attgctgagg agcttggcca gaaagatcaa atcaatcgcg cgctcgataa ctgccataag
     5281 gcggggatcg gcctgatatc gatgaagcag gtcgctggca ataccaagct cgatgagatt
     5341 ggtcgg
//
LOCUS       DATAQL010001149         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2864546, whole genome shotgun sequence.
ACCESSION   DATAQL010001149 DATAQL010000000
VERSION     DATAQL010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864546"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VHS97_19875"
     CDS             complement(<1..500)
                     /locus_tag="VHS97_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450525.1"
                     /translation="MISKSQQTTLRLMIIHFLLAVCFWASPAFGQDFHFDSSMSRPVL
                     ENYLDRSISFTELLHDDLEQPRNSRGVDPRDNMRLILGSKAKFVGRALMVWGRERNLA
                     GFLRTAKPYAEALHRADPEIILQAAAFEIVTRAVESVPVPDDVFIEFGQPVEKRNFRY
                     QDMLYA"
     gene            847..3333
                     /locus_tag="VHS97_19880"
     CDS             847..3333
                     /locus_tag="VHS97_19880"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450526.1"
                     /translation="MLGSVRGFQVPLSPLAILVGFALVSLCANAAATEPTSQAIAPAS
                     GSDEKAPAKAAAAALNKPGQASDPATSPEFFAQPGDEPAQPFVPLRPSTVDDRRRIEA
                     VRLYSTARALEDQRAWSDAVTLLQEALKLDPDSLAIARRLSRIYIGALGRPDKALQFG
                     KRVLAVEPGDTETLSQLVDYYKKNDPPGAETILSEVLANPKLDAQAPGRLLANFELGK
                     LYSGRLHQTEKAADAFAKVLAALDDKSANRLSPADLARVLGNDPATAYLNFGLVFLEA
                     KKYDLAEKALERGLIYDEDNSQISLQLADILVKLNKGDQALALIDRHIERQTPFVEAY
                     ELLAKVLKSLNREKELTPRLEAAARRDSKNVPLQYFLADRYRETGQNDKAELLYKELL
                     TSQPTPQTYRALVASLLKRKKAADLLRVMSEAWLRPESQEAIKPQLAVAAADDAMAEA
                     ILDEGVKQTTAAAHSLTRPAQIILSVIANNPGPNSSNKNLRLEKLLHLERLMLEQNPT
                     ELIYLEIVDTLRRLGNYAEAATAIEQLIAKYPNAKSVRILTFLADYHRRAGHIEAAKS
                     TLQAAMKLDVGDVESQLLFAEGLQQIGQIDDAIQILKKAIAKEPNNPLYELTLVDFLS
                     KLGRDDEAIKVLEGMLKRHADNDQVMSFIRQKLSVIYVNKGDYAKGEAQLELLLERNP
                     DDPGPNNDLGYLYAEQGKNLDKAEMMIRKALQEEPESFSYLDSLGWVLFKRGKLKEAL
                     EPMRKAAEQMKVYMEQKGSGPDATIYEHLGDIYFQLQQLEKASDAWREAAKAAATAVP
                     VDKRLTEIRKKLETLEKLGPMPKPSSDRTP"
     gene            3504..>3916
                     /locus_tag="VHS97_19885"
     CDS             3504..>3916
                     /locus_tag="VHS97_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3450527.1"
                     /translation="MAGHSHSANIAHRKGLVDAKRGKLFTKLCRAVYVAARNGGGDPA
                     ANMRLRYSIDKARSFSVPKENIERSIKKATGELGGENFEEVVYEGYGPGGVAVLCEAL
                     TDNRNRTASELRRVFELAGGNLGASGCVGYLFNFKG"
BASE COUNT          884 a         1142 c         1138 g          752 t
ORIGIN      
        1 ttggcgtaga gcatgtcttg atagcggaag ttgcgttttt caaccggttg accgaattcg
       61 atgaaaacgt cgtcgggcac agggactgac tccacggccc gggttacgat ctcgaacgcg
      121 gccgcctgca ggatgatttc cggatccgcc cggtggagcg cctcggcgta aggcttcgcc
      181 gtgcggagga agccggcgag atttctctcc cttccccata ccatgagagc ccggcccacg
      241 aatttggcct tggagccaag gatcaggcgc atgttgtcgc gcggatcgac cccccggctg
      301 ttacgcggtt gttccagatc gtcgtggagc agctcggtaa aggagatcga gcggtcaaga
      361 tagttttcga gcaccggccg cgacatcgac gaatcgaaat gaaagtcctg gccgaacgcc
      421 ggactggccc agaaacagac ggccaagagg aagtggataa tcatcagacg gagcgtcgtt
      481 tgttggctct tggagatcat ggcacttccg gttgctggtg gaatgaaagc gtttctcctc
      541 cacggtgctt ggcggtccca cggctcgttg gtggggaagt cgttgattgc gcccgtacat
      601 gcattggagg ccgatagcag aataggcgag ttttcgacaa aggacaaagg gttgcaggtc
      661 ttggcagtcc cggcggcgac ttgttaccat agcttaactt cgtgggagat cggggctaag
      721 atccagacga tggaacgtcg tcccgccggt tacgaccccg agttccgacc ctcggtatcc
      781 ttgcgaagat ttctgtagag tgtcgcccga cgcggcgaca attcttctgg acgaggagac
      841 acgaccatgt tggggtcggt tcgagggttt caagtgcccc tgtcgccgtt ggcgattctg
      901 gtcgggtttg ctttggtttc gctctgcgca aatgcagctg ctaccgagcc gacttcgcaa
      961 gcgatcgcgc ctgcctcggg gtccgacgag aaggcaccgg cgaaagcggc tgcggccgca
     1021 ttgaacaaac cggggcaggc gagcgaccct gcgacttctc ccgaattctt cgcgcagccc
     1081 ggtgatgaac ccgcacagcc attcgtaccg ttgcgtccct cgaccgtgga tgatcggcgg
     1141 cggatcgagg ctgtgcgcct ctacagcact gcgcgggcac ttgaagatca gcgtgcctgg
     1201 tccgacgcgg tgaccttgct ccaagaagcg ctcaaacttg atcccgactc gctcgccatc
     1261 gcgcgacgct tgagccggat ctacattggt gccctggggc gcccggacaa agcactgcaa
     1321 ttcggcaagc gcgtcttggc cgtcgagccg ggcgacaccg aaaccttgtc gcagctggtc
     1381 gattactaca aaaagaacga tccgcctggc gccgaaacga ttttgagcga agtgctggcc
     1441 aaccccaagc tcgacgcaca agccccgggt cgtcttctgg ccaatttcga attggggaag
     1501 ctttactccg gacgcctcca tcaaacggaa aaggcggccg atgcgttcgc caaggttttg
     1561 gctgcactcg atgacaagtc cgccaaccgg ctctctccag cagatctggc tcgcgttctg
     1621 ggcaacgatc cggcaaccgc gtacctcaat tttggcttgg tcttcctcga ggccaagaaa
     1681 tacgatctcg cggaaaaggc tctcgaacgc ggcttgatct acgacgagga taattcccag
     1741 atatcgctgc aactggccga tatcctggtc aagctcaaca aaggcgacca ggctctggcg
     1801 ctcatcgacc gtcacatcga gcgtcagacc ccttttgtgg aggcctacga gctcctggcc
     1861 aaggtattga agtcgttgaa tcgcgagaag gagctcactc ctcggctgga agctgcagcc
     1921 cggcgcgatt ccaagaacgt gccacttcag tacttcctgg cggatcgcta ccgcgagacc
     1981 ggccagaacg acaaggcaga gttgctttac aaagagcttt tgacctcgca gccaacccct
     2041 cagacctatc gcgcacttgt ggcgtcgctc ttgaaacgga agaaagccgc cgatttgctc
     2101 agggtgatga gcgaggcgtg gctgcggccc gaaagccagg aggcaatcaa accgcagctc
     2161 gcggtcgcgg ccgccgatga tgccatggct gaggccatcc tcgacgaagg tgtgaaacag
     2221 acgacggccg cagcccatag cctgacgagg cccgcccaaa tcatactctc ggtcatcgcg
     2281 aataaccctg gcccaaactc atcaaacaag aacctccggc tcgaaaagct cttgcatctt
     2341 gagcgcctga tgctcgagca aaatccgacc gaactaatct acctcgagat tgtcgacacc
     2401 ttgcgacggt tgggaaatta cgccgaagca gcaactgcca tcgagcaatt gatcgccaag
     2461 tacccgaacg ccaagtcggt ccggattctg actttcctcg ccgactacca tcgccgcgca
     2521 ggacacatcg aagccgccaa gtcaacactt caagctgcaa tgaagctcga tgtcggcgac
     2581 gtggaatcac agcttttatt cgccgaaggg ctgcagcaaa tcggccagat cgacgacgcg
     2641 atccagatct tgaaaaaagc gatcgccaaa gagccgaaca acccgcttta cgagctgacc
     2701 ttggtcgatt ttttgtccaa actcggacgt gatgacgagg cgatcaaggt gctcgagggg
     2761 atgctcaagc gccatgccga caacgaccag gtcatgtcgt tcattcgcca gaagctctcg
     2821 gtcatttatg tgaataaagg cgactatgcc aagggagaag cgcagctcga gctccttctc
     2881 gagcgcaatc cggacgatcc cggccccaac aatgaccttg gttaccttta tgccgagcag
     2941 ggcaaaaacc ttgataaggc cgaaatgatg atccgtaagg ccctgcaaga ggaacctgag
     3001 agcttctcgt atctcgatag cctggggtgg gtcctgttca agcgcggcaa gctcaaggag
     3061 gctctggagc cgatgcggaa agccgccgaa cagatgaaag tctacatgga acaaaaaggg
     3121 agcggccccg acgccacgat ctacgagcac ttaggcgaca tttacttcca gctccagcag
     3181 cttgagaagg cgagtgacgc atggcgagaa gccgcgaaag ccgccgcaac ggccgtcccg
     3241 gtcgataagc ggctcacgga gatccgcaag aaactcgaaa cactggaaaa actcggcccc
     3301 atgcccaaac cgtcatcgga taggacaccg tgaagtgtgg tgaatgatcc gtccgcattc
     3361 gtcaccaaaa aacggatcgg tttcgcccac gacatgatcg ggtcatcggg tagttgggcc
     3421 atgagtatgg taaaggtcgc cgcgggaagg taatactttt ccctcctgac cacccgctac
     3481 ggactaatgg agagataata acgatggcag gacattccca ttcagccaac atcgctcatc
     3541 gcaaagggct ggttgacgcc aagcgaggta aactttttac caagctctgc cgggcggtct
     3601 acgtggctgc gcgcaacggt ggcggcgacc ctgctgccaa tatgcgtctg cgctactcga
     3661 tcgacaaggc gcgctcgttc agtgtcccca aagagaatat cgagcgctcg atcaagaagg
     3721 cgacgggcga gcttggcggc gaaaactttg aggaagtcgt ctatgaaggc tacggccccg
     3781 gcggtgtagc cgtgctctgc gaagccctga ccgacaaccg aaaccgcacc gcaagtgagc
     3841 tccgccgcgt tttcgagctt gccggcggta atctcggcgc cagcggctgc gtcggttatc
     3901 ttttcaactt caaggg
//
LOCUS       DATAQL010001150         6236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1491345, whole genome shotgun sequence.
ACCESSION   DATAQL010001150 DATAQL010000000
VERSION     DATAQL010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491345"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VHS97_19890"
     CDS             <1..1298
                     /locus_tag="VHS97_19890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX3450528.1"
                     /translation="SENIEGRRREGIRRRFGRLALSSINDLSLQLVGSTRAGDYAMIR
                     FRVAINTLGPALAMACVVASVRGDQVQPEDVLKSHGLSIKRGGSTYVLAAESDIQLKL
                     SEAQRIFKQLSIALRQRHDFEQAVDQRRRLVPGLLEERIYLNQQLRSVNRQDVVTHNQ
                     IVARLNEINDQLSLVQITTADPRRKQDIDNEVAQSRGRYIQAVLDLRALVDSTLRQYA
                     ELAQDETIKAALEALKSKTKSPLKLGPSRGFDESVKQLAIREKSVLSRAVEMRHRGGV
                     FEVDVTLNGRETIPMIFDTGASLVTLSSEFAAKIGLTPEASNQTVQLHDASGGITAAK
                     MMTIPTVRVGPFTVNNVDCAVLPPNKRDVPLLLGQSFINQFTHKVDNGRLLLSKVETP
                     EPQANSATTPKKTTKAKRSSKAATGASAPATKGDSDGSF"
     gene            1363..2592
                     /locus_tag="VHS97_19895"
     CDS             1363..2592
                     /locus_tag="VHS97_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX3450529.1"
                     /translation="MTRITVALLISLLSFHTREARAVDTTDQIATLVKGNSEFALDLY
                     ARISQGDGNRFLSPFSISTALTMTYAGAQEETAVQIAKTLRFMLPPGQLHPAFHRLIA
                     ELHGRTSTQSAPDHPPDVQLFTANALWTQTGERILPDFQKRIEVNYQGGLYTVDFHHA
                     PDQARRTVNTWVEERTKGKIRDLLKSSHIDPTTILVLTNAIYFNARWATPFSQDKTAQ
                     EFFHASVTDVAPVAMMKHSGRFRYFDEGTFQALELPYKGDTLSLVIFLPKANDGLGRL
                     EASLSAARIGNWLTKLSSHRVDISLPKFKMTSECELSDALSEMGMSVAFKPGAADFSG
                     ITGTRELAISAVVHKAFVEVDEKGTEAAAATGVVAVRAAAIAQPPVIFRADHPFFFLI
                     RDIRTASILFVGRLVKP"
     gene            complement(2638..3981)
                     /locus_tag="VHS97_19900"
     CDS             complement(2638..3981)
                     /locus_tag="VHS97_19900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450530.1"
                     /translation="MKFRIRSIFAKIVLWFVVTVGLSLVGFLGTSLLVSATLTGRATN
                     FARINALFLDDARRAYEDGGPVRLAAYLNRLDTYSDSQHFLTDGRGIDLVSGVDRSDL
                     RNVPRRVARRLIPVWLLNRTGPIVRVRMSDDRRYRLISVFPPPPRLGAWDTFEYFLWL
                     PVLIGALCYVLAVHLASPLRNLRRVVDRFGRGELGIRFHLARRDEIGELARAFNRMAD
                     HITTLLSAERRLLQDVSHELRSPLARLGFAVELAKTSNDREAALNRVRKEADRLGQLV
                     DELLELTRSEGDPAARNMDEFELGELIEDLVEDSSVEAEAQGCRLVARIDDRPVLFGE
                     RELVRRACENVLRNAIRHAPAGSSIEVSLSARDRQAWIKIRDHGPGVPPDALGEIFHP
                     FYRVEKDRGRSSGGIGLGLAITRRAIELHHGRVTAQNANPGLAVEIELPMDRSNQ"
     gene            complement(3978..4667)
                     /locus_tag="VHS97_19905"
     CDS             complement(3978..4667)
                     /locus_tag="VHS97_19905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3450531.1"
                     /translation="MLIDDDTELCELMQEFLGSRGIRIDAVHDGRRGLAQAFDRVYDL
                     VLLDVMLPGLDGFELLRQVRRRSEVPIIMLTARTAKDDRVMGLEAGADDYLPKPFDPD
                     ELVARIRAVLRRSGRFPAPGVEILEVNGVGLSPATRQVWSDGRTVDLTTIEFDILDLI
                     IRSAGRVVSRHELAAAIHQRPASPLDRSLDVHVSHLRKKLGRRGAVIRTVRGVGYLFR
                     AELHERNETAR"
     gene            complement(4864..5703)
                     /locus_tag="VHS97_19910"
     CDS             complement(4864..5703)
                     /locus_tag="VHS97_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450532.1"
                     /translation="MHGTLDRFAVAARQRRRRHASSKATVRCEVLEERSLLTAFSAPG
                     AIGLRESRAAETSLFGAGRQNGMFGRGSLGLGGGMTNPTLFLTAPLLDSAGGTTTPPS
                     PSVMSSTAAQTAFQTLQTDLKNDTPSGAQATHASIGAVQDDLDAIRKGTLTGTAAVTQ
                     VQTDAAAVLSSMGLTAAQVTQIQTDQQAVQTALEANSSSSTPSTSSNSPASTSSTSST
                     ATIAATESTLQSVSAYLIGIPGVSTAVGIRGMGAGGFGGGRGRGFGGGFGGGIGGGLR
                     GGF"
BASE COUNT         1316 a         1938 c         1804 g         1178 t
ORIGIN      
        1 aaagcgaaaa catcgaaggc cggcgccgcg aagggattcg gcggagattc gggcgtctcg
       61 ccctgagctc gatcaacgac ttatcgcttc agctcgtcgg ttcgactcgt gccggagact
      121 atgccatgat tcgctttcgg gtcgcgataa atacgttggg accggcgctg gcgatggcct
      181 gcgtggtcgc gtctgttcgc ggcgatcaag tccagccgga agacgtgctc aaaagccacg
      241 gcctcagcat aaagcgcggg ggctcgacct acgtcctggc cgctgagtca gatatccaac
      301 tcaagttgag cgaggctcaa cgcattttca agcaattgag catcgcgctg cggcaacgac
      361 atgacttcga acaggcggtc gatcagcgga gaagattagt acccggactt ctcgaggaac
      421 ggatttactt gaaccagcag ctccgatcgg tcaacaggca ggatgtggtg acgcacaacc
      481 agatcgtggc gcgattaaac gaaatcaacg atcagctcag tttggtccaa atcacgaccg
      541 ccgacccacg gcgcaagcaa gacatcgaca acgaagtagc ccaaagtcga ggacgctata
      601 tccaggccgt cctcgacctg cgcgccctcg tcgactcgac ccttcgccaa tacgccgagc
      661 tggcccagga cgagacgatc aaagccgcac tcgaggcact caaatccaag acgaaatctc
      721 cgctgaagct gggaccgtcc cgcggattcg acgagagcgt aaaacagctc gccatccgcg
      781 agaagtccgt gctcagccgg gccgtcgaga tgagacacag aggaggcgtc ttcgaggtcg
      841 atgtgacttt gaatggcagg gaaacgattc cgatgatttt cgacaccggt gccagcctag
      901 tcaccctatc gagcgaattc gccgccaaga tcggcctgac tcccgaggca agcaaccaga
      961 ccgtccagtt gcacgacgcc agcggcggga tcaccgcggc aaagatgatg acgattccca
     1021 cggtgcgcgt cggtccgttc accgtcaata atgtcgattg cgctgtcttg ccccccaata
     1081 agcgcgacgt acctctcttg cttggtcaga gctttatcaa ccagttcacg cacaaggtcg
     1141 acaatggccg gcttctgttg tcgaaagtcg agacgccgga gccgcaagcc aactcggcga
     1201 ccactcccaa gaaaacgaca aaggcaaagc gttcaagtaa ggctgcgacc ggtgcaagcg
     1261 caccagccac caagggggac tcggacgggt cgttctaaaa agcgttttgc cgctcgagat
     1321 cgactcccgc cgccctacca gctcgcccgt gggtacgctt cgatgactcg aatcacagtc
     1381 gcgctcttga tttcgctact tagctttcac actcgcgagg caagggccgt ggacacaacc
     1441 gaccaaatcg ccactctggt taaaggcaac agcgaatttg cgctggacct ctatgcccgg
     1501 atcagccagg gagacggcaa ccgcttcctc tcgccgttct caatctccac tgcactgaca
     1561 atgacctatg ccggagctca agaggaaacc gccgtacaga tcgccaagac tttgcgcttc
     1621 atgctgcctc cggggcagct tcacccggcg ttccaccgct tgatcgcgga actacacggt
     1681 cgcacttcca cccagagcgc gccggatcac ccgcccgatg tccagctctt cacggccaac
     1741 gcgctctgga cccagaccgg tgagcgaatc cttcctgatt ttcagaagcg aatcgaggtc
     1801 aactatcagg gggggctata cacggtcgat ttccatcatg ctcccgatca agcccggcgg
     1861 actgtcaaca cctgggtcga agaacggacc aagggcaaaa ttcgagatct gctcaaatcg
     1921 agccatatcg accccaccac catcctcgtc ctgaccaacg cgatctattt caacgcgcgg
     1981 tgggccacgc cattttccca agataaaacc gcacaagaat tcttccacgc gtcggtcacg
     2041 gatgtggccc cagtcgcgat gatgaagcat tcgggccggt tccggtattt cgatgaggga
     2101 acgtttcagg cccttgagtt gccctacaag ggcgacacgc tctcgcttgt gatcttcttg
     2161 cccaaggcga atgatgggct tggccgcctc gaagcgtcgc tgagtgccgc caggatcggt
     2221 aactggttga ccaagctctc gtcgcaccgc gtcgacatca gccttcccaa gttcaagatg
     2281 acctcggagt gtgagctctc ggatgcactc tcggagatgg gcatgtccgt ggcgttcaag
     2341 ccgggcgcgg cggatttctc gggaatcacc ggcacgcgcg aactcgcgat ctcggccgtc
     2401 gtgcacaagg catttgtcga agttgacgaa aaagggaccg aggcggccgc cgcgacgggc
     2461 gtcgtggcag tcagggctgc cgcaatagct cagcctccgg tcatctttcg tgcagaccac
     2521 cccttcttct tcctgattcg agatatccgc accgccagca ttctttttgt cgggcgcctg
     2581 gtgaagcctt gacgaccgaa gcacccggtg gcgcgttgcg ctcgctcgcc gcggccgtca
     2641 ttgattggat cggtccatcg gcagctcgat ctcgacggcc agtccggggt tggcgttttg
     2701 cgcggtcaca cggccgtgat gcaattcgat ggcgcgccgc gtgatcgcca gacccagacc
     2761 gattccgccg ctggagcggc ctcggtcttt ttcgactcgg tagaacgggt gaaaaatctc
     2821 ccccaatgcg tccggcggaa ctcccggacc gtgatctcgg atcttaatcc aagcttggcg
     2881 gtcgcgcgcc gacaatgaaa cctcaatgga actgcccgct ggagcgtgcc gaatggcgtt
     2941 tcgcagcaca ttctcgcaag ctcgccgcac gagctcacgc tcgccgaaaa gaacgggcct
     3001 gtcgtcgatc cgggcgacca gccgacatcc ctgtgcttcg gcctcgacgg aactgtcttc
     3061 caccagatct tcgatcagtt cgcccagctc gaactcgtcc atatttctgg ccgccggatc
     3121 gccctcagat cgggtgagct ctagcaactc gtctacgagt tgtccgaggc ggtcggcttc
     3181 cttgcggaca cggttgagtg cggcctcccg atcgttgctg gttttcgcca gctcgaccgc
     3241 gaagccaagc cgcgccaggg gcgagcgcag ttcgtgggaa acgtcctgaa gaagccgccg
     3301 ctcggccgag agcaaagtag tgatgtggtc ggccattcgg ttaaaggctc gtgccagctc
     3361 gccaatttcg tctcgtcgag ccagatggaa ccggatcccc aactcgccgc gtccgaaccg
     3421 gtcgaccacc cggcgcaggt tgcgtagcgg cgaagccagg tgaacggcca gcacgtagca
     3481 aagtgcgccg atcaagacgg ggagccagag gaagtactca aaggtgtccc acgctccgag
     3541 ccgtggggga ggaggaaaca cactgatcaa acgatagcgg cggtcgtcgg acatccttac
     3601 ccggacgatc gggccggttc tgttaagtag ccacactggg atcaagcggc gcgcgacgcg
     3661 gcgtggtacg ttccgcagat ccgagcgatc aacgccgctg actaagtcga tgccgcgtcc
     3721 gtcggtaagg aaatgttgcg agtcggagta ggtgtccagg cgattgaggt acgcggcgag
     3781 ccggacagga cccccgtctt cataggcacg acgcgcgtcg tcgagaaaga gagcattaat
     3841 ccgtgcgaag ttcgtcgcac gaccggtgag cgtagcggag acgagcagag aggttcccag
     3901 aaaaccgaca agcgagagac caacggtgac gacaaaccat aagacgatct tggcgaagat
     3961 cgaccttatc ctgaacttca tcgggccgtc tcgtttcgct cgtgcaactc ggcgcggaag
     4021 agatatccta cgcctcgcac cgtgcgtatc acagcccccc gccgtcccag tttcttgcgc
     4081 agatggctga catggacgtc gagggatcgg tccagcggcg acgcgggccg ctgatgaatt
     4141 gcggcggcaa gttcgtggcg cgacactacc cgtccagccg agcgaatgat tagatcgagg
     4201 atgtcgaact caatggtggt cagatccacg gttctcccat ctgaccatac ctggcgggtg
     4261 gccggcgaca gcccgactcc gttgacttcc agaatttcca cacccggcgc gggaaaccgg
     4321 ccggaacggc gcagcaccgc ccgaatccgc gcgaccagct cgtcgggatc gaatggcttg
     4381 ggaaggtagt cgtcagctcc cgcctccagt cccatgaccc ggtcgtcctt ggcggtccga
     4441 gccgtaagca tgatgatggg cacctcgctg cggcgtcgaa cttgcctcaa tagctcgaat
     4501 ccgtcgagtc ccggcagcat tacatcgagc aagacaaggt cgtacacgcg gtcgaacgct
     4561 tgggccaagc ccctgcgacc gtcatgaacc gcatcgatgc ggatgccccg cgagcccaga
     4621 aattcctgca tgagctcgca gagttcagta tcgtcgtcga tcaacaatac ggaaagatcc
     4681 gactccgcgg acgctggtct caaatcgttg ttcatcgtcc cgctgacttc gctcgaggaa
     4741 gagactatct gggtttgcat acaacggact gaagagatcg ggctgactcc ggcgagtgca
     4801 accaaaactc ggtggacctc cgcgcagatc ccccgagtcg ttcccagcta acattgtgcc
     4861 gggttagaag ccaccacgca gtcctccgcc gataccgcca ccgaatccgc ctccgaagcc
     4921 gcgtccgcga ccgcccccga agccacccgc gcccatgccc cgtatcccca cagcagtgga
     4981 tacgccgggt attccaataa ggtaggcact gactgattga agcgttgact cggtggctgc
     5041 gatggtcgcc gttgacgagg tcgatgacgt tgatgctggc gaattcgatg acgtcgacgg
     5101 agtcgaacta ctggaattcg cctccaaggc agtttgaacc gcctgttgat cagtctggat
     5161 ctgggtgacc tgggctgcgg tcaatcccat gctcgaaagc acggcagccg cgtcggtctg
     5221 aacctgcgtg acggcagcgg tgccggtcaa tgtccccttg cggattgcgt ccaggtcgtc
     5281 ctggaccgcg ccgatcgagg catgcgtggc ttgagcgccg ctgggtgtgt cgttcttgag
     5341 atcagtctgc agggtctgga aggccgtttg ggccgccgtc gacgacatta cgcttggaga
     5401 aggcggggtg gtagtccctc ccgcactgtc caggagcgga gccgtcaaga acagggtcgg
     5461 gttcgtcatg cccccgccca agcccaacga ccctcgtccg aacatcccat tctgcctacc
     5521 agccccaaac aaactcgtct ccgccgccct tgattcccgc aacccgatcg caccgggggc
     5581 gctgaacgcg gtcaaaagag acctctcttc caaaacctca cacctcaccg tggccttcga
     5641 cgacgcatga cgacggcggc gttgccgagc ggcgacggcg aatcgatcca atgttccgtg
     5701 catggactca tcctccttga cgaactgtct ctcttaacta tctatgtaaa aatggtttgc
     5761 gaccagagaa cgtaccgcat ggaaaaacag ctacgttttt tagcgagaaa ctcgtgcatc
     5821 tcatcttgcc gccgtctcgc tgcctccata tgggaatcga aattcgcatt tttccctcgt
     5881 caccggggcc aggcaattta caaaacttaa cgccatgatc gacgaccgga ttccgctggc
     5941 ggttgcgcta cctcagttcg tggttcacgc ctgaaacagt aggcggaatt gttgagctcc
     6001 gtcacacggc ggcatcgctc aattaaaccc cgccggcagg tgtgagtcgc agcatttcga
     6061 gacgattttg gcgaagggaa gggagcggca atgcgatcta gcaaacgaat tcgacgaggt
     6121 gcattctgtg tcggtctggc gatgctcgct ggagccggtg gggcgctttt gagtgccggc
     6181 tcgcccccgt catcaaacac ccaaggcaaa gtggatcgga gcgacttgat cgttca
//
LOCUS       DATAQL010001151         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1641628, whole genome shotgun sequence.
ACCESSION   DATAQL010001151 DATAQL010000000
VERSION     DATAQL010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641628"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..887
                     /locus_tag="VHS97_19915"
     CDS             69..887
                     /locus_tag="VHS97_19915"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3450533.1"
                     /translation="MIELYHHGSSVCAAKVRFVLMEKGIPWESRYLDILKGEQFHPDY
                     LKLNPMAVVPTLVHDGEVLVESTVICEYLDDAFPEPPLKPASALERARMRLWTKVVDE
                     HIHPACKYITYAACHRHIIRRLPPEKFAQYMKGPEGEVQTRVAGDPNWVESKRAIVAT
                     GLEAPGVASKFRMYDDYLQKMENTLERQAWLAGATFTLADIAMTPYLNRLDMLGMSDM
                     WVGTRPRLTDWFERIKARPTFKPCFLDYCPPDLTADLKTYGSQSWPEVRAILSA"
     gene            complement(948..1868)
                     /locus_tag="VHS97_19920"
     CDS             complement(948..1868)
                     /locus_tag="VHS97_19920"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3450534.1"
                     /translation="MVADVDSPSYFVATAAAKLGFFAQEGIDVEFEPVYGAKYGPERL
                     RDGTLHFLGGPAYVATRAFPAWKGAKLLCALSQYSYWFMAVRADLKIKRGDLSAVKGL
                     RIACSPTFPEMGLRYMLAEAGIDPARDNVGIVASPTAHGKEHLKGRDGVVAIEQGVAD
                     GYWGNGMRVAIGESFGVAKLHLDLRRGDGPPGARWYNFAALTTTEQFVREQPQVAAAA
                     IRAIVKTQKTLQLDPGLASKVGQELFPAEEAALIEDLIARDTPFYDATISAEAVNGLN
                     KFARANGLIVEPIPYDTLVASEFRHLWSGQ"
     gene            complement(1899..>2188)
                     /locus_tag="VHS97_19925"
     CDS             complement(1899..>2188)
                     /locus_tag="VHS97_19925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3450535.1"
                     /translation="APEVPTVDEAGLPGFYVSNWHGVWVPKGTPTNVVASLNAAIVEA
                     LAEATVQRRLTELAQELPPRAAQTPEGLATLQRAEIEKWWPIVKAANIKAD"
BASE COUNT          426 a          670 c          648 g          444 t
ORIGIN      
        1 aagccgcttg agccggcccg attgatcccc cgcgccggat gacgcgcggg ataaaggaaa
       61 acaaatcgat gatcgaactg tatcaccatg gctcttctgt ctgtgccgcc aaagtccgtt
      121 tcgtcttgat ggaaaagggc atcccgtggg aaagccggta cctcgacatt ctcaagggtg
      181 agcaattcca tcctgattat ctcaagctca acccgatggc ggtcgtgccg acgctggttc
      241 atgacggcga ggtgctggtc gagtcgaccg tgatttgcga atacctggat gatgcctttc
      301 cggaaccgcc gctcaagcct gcatcagcac tggagcgcgc acggatgcgc ttatggacga
      361 aggtggtcga tgaacatatc cacccggcct gcaaatacat cacctatgcg gcgtgccatc
      421 gtcacatcat acgacgactg ccgccggaga agtttgccca gtacatgaag gggccggaag
      481 gtgaggtgca gacgcgcgtc gccggcgatc ccaactgggt cgagagcaag cgagccattg
      541 tcgccaccgg actggaggca cccggcgtcg cttcaaagtt ccgaatgtat gacgattatc
      601 tgcagaagat ggaaaacacg cttgagcgac aggcttggct cgccggcgcg acgttcaccc
      661 tcgccgacat cgcgatgacg ccttatctga accggctcga tatgctggga atgtcggaca
      721 tgtgggtcgg cacgcgtccg cgcctgacgg actggttcga acgcatcaag gcgcggccaa
      781 cgttcaagcc ttgttttctc gactattgtc cgccggacct tacggcagac ttgaagacct
      841 atggctcgca gagctggccg gaggtgagag ccatactctc cgcttagtcg gccgatgttt
      901 ccgtgctttg gggcgccact gatatcgaat cgcagcccac tactggttca ttgcccgctc
      961 cagaggtggc gaaattctga ggccaccagc gtgtcatagg ggatcggctc cacgatcagc
     1021 ccatttgccc tggcaaattt gttcaatccg ttgactgctt ccgccgaaat agtcgcgtcg
     1081 tagaacgggg tgtcacgcgc gatcaaatcc tcaatcaatg cggcttcttc cgcggggaag
     1141 agttcctgtc cgactttgct ggcgagccca ggatccagct ggagcgtttt ttgggttttc
     1201 acgatcgcgc ggatcgcggc tgcggcgacc tgaggctgct cccgcacgaa ctgttccgtc
     1261 gtcgtcaggg ctgcgaaatt gtaccagcgc gcgccgggcg ggccgtcccc acgtcgtaga
     1321 tcgaggtgca gtttcgcaac cccgaaactc tcgccgatgg cgacgcgcat gccgttgccc
     1381 cagtagccat cagcaacgcc ttgctcgatg gcgacgaccc cgtcacgtcc cttcaaatgc
     1441 tccttcccgt gggcggtcgg gctcgcaaca atgcccacgt tgtcgcgcgc aggatcaatc
     1501 ccggcttcgg ccagcatgta tcgcagtccc atttcgggaa atgttggtga gcaggcgatc
     1561 ctcaatcctt tgacggcact aaggtcaccg cgtttgatct tgaggtcggc gcgcaccgcc
     1621 atgaaccaat acgaatactg ggacaacgcg cagagaagtt ttgctccttt ccaggctggg
     1681 aaagcccgcg ttgccacgta ggcggggccg ccgaggaaat gcagggtccc gtcgcgcagg
     1741 cgttccggtc cgtatttggc gccgtagacc ggctcaaact cgacgtcgat tccttcctgc
     1801 gcgaagaaac caagcttggc cgcagccgtt gccacgaaat acgaaggcga atcgacgtcg
     1861 gcaaccatga gccgatacat ccggaccact ccgcgggttc aatctgcttt gatgtttgct
     1921 gccttgacga tcggccacca tttctcgatc tcggcacgct gaagcgtggc gagcccttcc
     1981 ggggtctgcg ctgcgcgcgg cggaagttcc tgggcaagct ccgtcagccg ccgctgcacg
     2041 gtcgcctccg ccaacgcctc gacaatcgct gcgttgaggc tggcaacgac attcgtgggc
     2101 gtgcctttgg gcacccagac gccatgccaa ttcgagacgt agaagcctgg taatccggct
     2161 tcgtccacgg tgggaacctc gggcgcaa
//
LOCUS       DATAQL010001152         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5308855, whole genome shotgun sequence.
ACCESSION   DATAQL010001152 DATAQL010000000
VERSION     DATAQL010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5308855"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="VHS97_19930"
     CDS             complement(<1..1403)
                     /locus_tag="VHS97_19930"
                     /inference="COORDINATES: protein motif:HMM:NF023835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase-associated domain-containing
                     protein"
                     /protein_id="HEX3450536.1"
                     /translation="MMLAPVLTPRGLLILRETEDALGLESEEHPRLAEAFLRGPGHGL
                     LRLGANEVETVLPPVLSYWREFGARYVTALCAVSDIGERSTKPPVAVPVDGELDHMAA
                     AVPPMTGGEYLTTDVLAHLWRDMDAAFDAELADAGLSVQEFLKGRHPAWNLIGRVHFN
                     LAENRKDEVAPFAFLVTYTTRLSAAAKAQHVHLGKALQEYAGARNRERLLSLLLPIQR
                     AAEHCTWLKAMVDAGEIFHPLRWSPQQALQFLKDVPTLESAGVAVRMPASWRLNRPAR
                     AQVKATIGGRTPSQMGMDALLDFRMEVTLDGEKLSAAEIKRLLAQADGLVLVRGKWVE
                     VDHERLRRTLDEFEAVERRAASDGLSFGEAMRMLAGANIAEKDSGGQADIDWSQTVAG
                     TWLAETLAALRRPGGPARINPGKSLQGSLRSYQLAGVQWLYLLTQLRLGACLADDMGL
                     GKTIQVLSLLLVLKSET"
     gene            1533..>1805
                     /locus_tag="VHS97_19935"
     CDS             1533..>1805
                     /locus_tag="VHS97_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450537.1"
                     /translation="MPVSIGDIAEAFEFANAGGNMGEYLAVVCKRTGKIYYQTDFPDA
                     AEMNDELPDDINDEEKYVALPDKRELGLGKPLVLAFARQFLPDDFDD"
BASE COUNT          364 a          567 c          542 g          332 t
ORIGIN      
        1 ttggtctcgc tcttcagcac gagcagaagc gataggacct ggatggtctt gccgagcccc
       61 atgtcgtccg caaggcaggc cccgagcctg agctgagtga gcaaatagag ccattgcacg
      121 cctgccagtt gataagaccg caatgaccct tgaagggact tgccgggatt aatccgagca
      181 ggcccaccgg ggcggcgtaa ggcggccaat gtctccgcca gccaggtacc cgccacggtc
      241 tggctccagt cgatatctgc ttgtccaccg gaatccttct cggcgatgtt ggcgccagcc
      301 aacatgcgca tcgcctcgcc gaacgacagg ccgtcactag cggcacgccg ttcgaccgct
      361 tcgaattcgt caagggtccg gcgcagacgt tcatggtcaa cctcgaccca tttgccacgc
      421 accaaaacca gcccgtcggc ctgtgcgagc agccgcttga tttcggccgc cgaaagcttt
      481 tcaccgtcga gcgtcacttc catccggaag tcgagcaacg catccatccc catctgcgac
      541 ggcgttcgcc ccccgatcgt cgccttgact tgcgcacgcg cggggcgatt cagacgccag
      601 ctcgcgggca tccgcaccgc gaccccggcg ctttcgagcg ttggcacgtc tttgaggaat
      661 tgcagcgcct gctgcggact ccagcgtagc ggatgaaaga tctcgcccgc gtcgaccatg
      721 gccttcaacc aggtgcaatg ctcggcggcg cgctggatgg gcagcagcag cgacagcagg
      781 cgctcacgat ttctcgcacc ggcatattcc tgcaaggcct tgccgagatg cacatgctgg
      841 gctttggctg ccgccgaaag ccgtgtcgtg taggttacca ggaacgcgaa cggggcaact
      901 tcgtcctttc tgttctcggc gagattgaaa tggacgcggc cgatcaggtt ccaggcagga
      961 tgtcggcctt tgaggaactc ttgcacggag agcccggcgt cagccagttc ggcatcaaag
     1021 gctgcatcca tgtctcgcca aagatgggcc aggacatccg tcgtcaggta ttcgccgccc
     1081 gtcatgggcg gaacggccgc ggccatgtgg tccagttcgc catccaccgg aactgccact
     1141 ggcggcttgg tggaccgctc tccgatatcg gaaacggcgc ataatgcggt tacgtagcga
     1201 gctccgaact ctcgccaata cgacaacaca ggaggcagaa ccgtctcgac ttcattggcg
     1261 cccaggcgca acaacccatg tccgggcccc cgcaggaagg cttctgccag ccgaggatgc
     1321 tcttcggact ccagcccgag cgcgtcctcc gtctctctga gaatcagcaa accacgcggg
     1381 gtgagaacgg gcgctagcat catgatcgga gtactctttc gattcgcatt ggatgggagc
     1441 ggggattgcc agttgacggg gctgaccggg atgacggatc atatcgtggg tctggatccg
     1501 aatgacacct gatcgggcgg cagccgaaaa ccatgccagt gagtatcggg gacattgccg
     1561 aggccttcga gttcgccaac gccggcggca acatgggaga atatctcgcc gtcgtctgta
     1621 agcggaccgg gaaaatctac tatcaaaccg attttccaga cgcagcagag atgaacgacg
     1681 aactgccgga cgacatcaac gacgaggaga aatatgtcgc actcccggac aaacgtgagc
     1741 tggggctggg caagccgctg gtgctagctt ttgctcgcca gttcctgccg gacgatttcg
     1801 acgac
//
LOCUS       DATAQL010001153         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3781600, whole genome shotgun sequence.
ACCESSION   DATAQL010001153 DATAQL010000000
VERSION     DATAQL010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781600"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1577
                     /locus_tag="VHS97_19940"
     CDS             282..1577
                     /locus_tag="VHS97_19940"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3450538.1"
                     /translation="MIPINLVPVLFLLHAQAEPPPRPQPVVLKAARLFDGTSDQLVPN
                     GMVLIEGKIIKAVGPGLSVPEGSSIIDLGDVTLCPGFIDAHTHLTHERSADFNQGFVD
                     SMRREVAEQAILSTVYARRTVEAGFTTVRDVGSGDFLDVGLRNSIAKGIVPGPRMLVC
                     VHALGATGGHSDRNGLRHDLLPQRDATEGIANSPDRVREAVRYQVKYGADVIKFCASG
                     GVLSLADEVDTPQLTLGEMTALCDEAHRLRKKVACHSHGDQAAHDAVLAGVNSIEHGS
                     FLSTDTLKLMKERGTYLIPTLMAPDSIMPNLSKFPPEIAAKAVLANEAISKTFATAVK
                     LGVKVGFGTDSGVSKHGDNAREFELMVQYGMTPVDALKAATSVDAELLGLAKSIGTLV
                     PGKLADLVAVPGDATADIGATRHVVFVMKEGAVVVAKRH"
     gene            complement(2111..2443)
                     /locus_tag="VHS97_19945"
     CDS             complement(2111..2443)
                     /locus_tag="VHS97_19945"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX3450539.1"
                     /translation="MKRGEIWLVGLDPTQGHEQKGRRPVLIVSPEAFNRVTKVPVVLP
                     ITSGGNFARTAGFAVMLEGAGTKTTGIIRCDQPRALDLSARGGKKLESVPEAIVDEVL
                     AKLTPIFE"
     gene            complement(2443..>2735)
                     /locus_tag="VHS97_19950"
     CDS             complement(2443..>2735)
                     /locus_tag="VHS97_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450540.1"
                     /translation="VILWYNIQEHVMHTTNLRKVGGSIMLAVPPALLDVLHLRAGAKV
                     GLAVEGGRLVVEPQPRPRYTLDELLAKCDPKARRSNEDREWLDDRPVGGELI"
BASE COUNT          494 a          890 c          804 g          547 t
ORIGIN      
        1 ttgaacggcg acactttgcc ggcgacgacg ttcttgggcg atctcgaaat cttcgaacat
       61 atgctgcaca atcggctgtt cggcctcccg gttgtcgagc caagtgtatt tcaccgcgcc
      121 ggttcgtgat cgccatgcgg ccgtctgcgg ctcatgcgcg gggcgacttt gggctcaaaa
      181 aaaatcgctg cgcacgctca gcgactcgcg ctgcgtttgt cttttaattc tcgtcgccgc
      241 gaactctcat tcgacccaca cgtagcggag ccacccaggt gatgatcccg atcaacctcg
      301 tccccgtttt atttcttctc catgcgcagg ccgaaccacc gccgcggccg cagccggtcg
      361 tgctcaaggc ggcccgactt tttgacggca cttccgatca actggtcccg aacgggatgg
      421 tgctgatcga gggcaagatc atcaaggccg tcggcccggg gctctccgta ccggaaggat
      481 cgtcgataat cgatctcggt gacgtgacgc tctgccccgg cttcattgac gcccacaccc
      541 atcttaccca cgagcggtcg gccgacttca atcaagggtt tgtcgactcg atgcgccggg
      601 aagtcgccga acaggccatc ctctcgaccg tctatgcacg gcgaacggtc gaggccggct
      661 ttaccacggt gcgcgatgtc ggctcgggcg acttcctcga cgtcggactg cgaaactcga
      721 ttgccaaggg gattgttcct ggtccgcgga tgctagtgtg tgtccatgcg ctgggtgcca
      781 cggggggcca ttccgaccgc aacggcctcc ggcacgacct acttccccag cgcgacgcga
      841 ccgagggaat cgccaatagc cctgatcgcg tccgcgaggc ggttcgctac caggtcaagt
      901 acggcgccga cgtgatcaag ttttgtgcgt caggcggagt gttatcactg gccgatgagg
      961 tcgacacgcc ccaactcacg ctaggcgaga tgaccgccct ctgcgacgag gcccaccgac
     1021 tccgcaagaa ggtcgcctgt catagccacg gcgatcaggc cgcccacgac gcggtgctcg
     1081 ccggggtcaa ttcgatcgag cacgggtcgt tcctgtctac cgacacactg aagctgatga
     1141 aggaacgagg aacatacctg atccccacct tgatggcacc cgactcgatc atgccgaatc
     1201 tgtccaagtt cccgcctgag attgccgcca aggccgtttt agccaatgaa gcaatttcca
     1261 agacgttcgc cacagccgtg aagctgggtg tcaaggtggg cttcggcacg gactcgggcg
     1321 tgtcaaagca cggcgacaat gcccgcgagt tcgagctgat ggtccaatat ggcatgactc
     1381 ccgtcgacgc cctcaaggcc gcgacgtccg tcgacgccga gttgctcggg ctcgcaaaat
     1441 cgatcggcac cctggtgccg ggcaagctcg ccgaccttgt cgccgtgcct ggtgacgcga
     1501 ccgcagacat aggggcgacc aggcacgtcg tcttcgtgat gaaggaaggg gcggtggtcg
     1561 tggcgaaacg gcactgaacg caataccgta gaattgccat aggctcacat gcgaatagaa
     1621 attcatgatc ccctcccccc ttgtggggga gggccagggt gtagggcgtt cctcccgggt
     1681 acccggccgc gcgtattctc cggcacgtac ctgcacccac cccgacactc cgttagcgtg
     1741 aggggttttc gtcacaactt gagaaggctc gcggtggact tcgcccctca ccccagcccc
     1801 ctccccaggg gagagggagc cagtaacatt catcgggccg cgagtttggg cgacaatcgg
     1861 ccgtatttcc agctagtgac atgcatcggc cgcgagtgcg ctgtaggggc gtcgggcttt
     1921 ggccggcaat cggccgtatt tctggccagt aacatgcatc gtctgcacga gtagtgcagc
     1981 gagccctacg gttccttcct gctccgtgtt ccgaaatggt tcgccaccag tgcattacgg
     2041 acgcgccggc gaggcgtatt cttctgctcc gcggcaacgg aggggaaggg tggttgaccg
     2101 gcctttttct ctattcaaaa atcggcgtca acttcgcgag tacctcatcc acgatcgctt
     2161 cgggaacgct ttccagcttc ttgccgccgc gcgcgctgag atcgagcgcg cggggctgat
     2221 cgcagcggat tatgcctgtc gtcttggtcc cggcaccttc cagcatcacc gcaaaacccg
     2281 ccgtccgtgc gaaattcccg ccgcttgtga tcgggagaac aacgggcact ttcgtcacgc
     2341 ggttgaacgc ctccggtgac acgatcagga ccggccgccg tcccttctgc tcgtgtccct
     2401 gcgtgggatc aagtccgact agccagattt cgccgcgctt catcagatca gctcgccgcc
     2461 gacgggcctg tcatcgagcc attcgcggtc ttcattgctg cgccgagcct tcggatcgca
     2521 tttggccagc agctcgtcga gcgtatagcg cggccgcggc tgcggctcta cgaccagccg
     2581 gccgccttcc accgccagac ctactttcgc tcccgcccgc aggtgcagga cgtccagcag
     2641 cgccggaggt accgccagca tgatcgagcc gccgaccttc cgcaaattcg ttgtgtgcat
     2701 cacgtgttcc tgaatgttat accataatat aacat
//
LOCUS       DATAQL010001154         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1491745, whole genome shotgun sequence.
ACCESSION   DATAQL010001154 DATAQL010000000
VERSION     DATAQL010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491745"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1027..2439
                     /locus_tag="VHS97_19955"
     CDS             1027..2439
                     /locus_tag="VHS97_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX3450541.1"
                     /translation="MSTWFQYGFNWLFSEFLTRLGFLLALVLMAFLLRERRSPSSTIA
                     WLLVILLLPYVGVPLYIMFGGRKIRRMALKKKPIYHPAIGSHGGGPHDNVERLLDSYG
                     VPPASAGNMVELITSGVDAYERVMRLIEEARSTIHVTTYILGRDEGSRALVDLMTRRA
                     AEGVAVRLLLDDVGSWRVRRRHLAPLINAGAQVAYFMPMLHIPFRGRANLRNHRKVVV
                     VDSHIAVAGGMNLAWPYIGPSSSVGLWRDLCMVVEGPAVSELETLFASDWRFTTGQDP
                     PMAGLPGPDPGLSRMESSVVQVVASGPDVAGDPLYESLLALIFGARDRIWIVTPYYVP
                     DEMLARALNLAARRGVDVRVMVPARSNHVTADLARAGYLRDLYEAGGRILLYTPAMLH
                     AKAVVFDDEIAIIGSANMDMRSLFLNYEVALYVSSPAQVGEVAAWARSLMVDSRLELP
                     ALGWARELSENIVRLLSPLL"
     gene            2463..>3893
                     /gene="treS"
                     /locus_tag="VHS97_19960"
     CDS             2463..>3893
                     /gene="treS"
                     /locus_tag="VHS97_19960"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX3450542.1"
                     /translation="MAATESLWYKDAIFYEVYTRGFFDANGDGDGDLIGLTSKLDYLQ
                     DLGVDCLWLMPIYASPHKDDGYDIADFRQIHPTIGTVADFEGLTKAAHARGMRIIADL
                     VVNHTSDQHPWFQEARRDPSSPYRNYYVWSDTDQVYKGARVIFRDTETSNWTWDPVAR
                     QYFWHRFYSHQPDLNFDNPQVKKEILNVMAFWLDRGIDGFRVDAVPYLFEREGTNCEN
                     LPETHDFLKEMRQFVDSRYPGSLLLAEANQWPSDLLPYFGQGDEFHMAFNFPLMPRLF
                     MAIRREDQRPLIDIMHQMPDVPTSCQWALFLRNHDELTLEMVTDEERDYMYTEYARDP
                     RMRLNLGIRRRLAPLMENGRRQIELLHSLLFSLPGSPVLYYGDEIGMGDNVHLGDRGG
                     VRTPMQWTGDRNAGFSRANPSALYQAIILDPIYHYQAVNVEAQLASPTSLLNWVRRLI
                     KVRRAHKVFGRGNLEFIPCHNRHAVAY"
BASE COUNT          750 a         1196 c         1082 g          865 t
ORIGIN      
        1 gggctcgttc ctgtgcagcg accagtattg cacccgctat cttcccggcg cgcgaggcaa
       61 aggcgctccc gacagcgccg cctcgcatgt cggctttcgg tgtgtaatct ctccgaagcg
      121 ctccgaataa ttgcgatggg gcggttccga gataattcag gccttcgctc agcgcgtggc
      181 cgaggcttaa gcaagaaggc ccgtgcctgc gatcgggttt gactgaccct ggcttcactt
      241 gtgcgatgcg cctttcggac accactgttc acgtattatc cgcgccggct tgcgatgcag
      301 ttgaaaagga acgctcgccg ccaagaaaga tgatagagac gatcaagcca gcttggacgc
      361 tccgctcacc caaggaagat ctcatcggag gttatcggta caagttatgg ttcttttttt
      421 cttgatcgtt tcggtgcatt tccgcgagat ccgtggttcg aaaccaggtt tttcggggcc
      481 gtatgctttc actccaacac catagcgtac ttccggtcgt tcgatcgcag tttcggtgac
      541 ggtcctcgtc gatgcaggcc ctggctcgga agctcgccct gagagccaaa gctccccccc
      601 cgggttcaag agcttttcgg cggcaacccg cacggtccca acgaggcgcg ccaccaaaac
      661 caaaaacacc cacgtctttt caaccgcttc tttatgccga acaaaccaat tgttcccctc
      721 tcgatcccat cgaacctaca acgagggaat actgtacgta tccgcacgct agcgagaaat
      781 ctgattgctg tcaaccaatc gtgtaaataa tcgtctaaga cgatcttaat aacgcttaat
      841 tagtcaagta caagcattgt aaagctcgac tttaatcagg gcaggccaag ctcattttgc
      901 ctcggactcc agaaagaggc agcggacaac cttggccgtc tggcttgatc tgataagatc
      961 gaccagcctt caggcgacgg cccattttgt ggagcaagtt gtctgggccg aggagtctct
     1021 ggcgcggtgt ccacctggtt tcagtacggc tttaactggc tcttttccga gtttttgacg
     1081 cgcctgggct ttttgctggc cctggtgctc atggccttct tgctccgcga gcggcgctca
     1141 ccctccagca cgattgcctg gctcttggtg atcctgctgc tgccttatgt aggcgttcca
     1201 ctttacatca tgttcggcgg ccgcaaaatc cgccggatgg ccctcaaaaa aaagcccatc
     1261 taccaccctg caattgggtc gcacgggggc ggtccacacg acaacgtcga acggttactg
     1321 gattcgtatg gcgttccgcc cgcaagcgct ggcaacatgg tggagttgat aacctccgga
     1381 gtcgacgcgt acgagcgcgt gatgcggctc atcgaggagg cacgttcgac catccacgtc
     1441 acgacctaca tcctcggccg tgacgaaggg agccgagcac tggtcgacct gatgactcgc
     1501 cgggcggccg aaggcgtggc cgtccgtttg ttactcgatg atgtcggttc gtggcgcgtt
     1561 cgacggcgac acctggctcc cttgatcaac gccggcgccc aggttgctta cttcatgccg
     1621 atgctgcata tcccatttcg cggtcgggca aacctgagaa atcatcgtaa ggttgtggtt
     1681 gttgacagcc atattgctgt tgccggcggc atgaaccttg cgtggcccta catcgggcca
     1741 tcgagttctg tgggcctgtg gcgggactta tgcatggtcg tggaagggcc ggcggtctcg
     1801 gaactcgaaa cgcttttcgc gtcggactgg cggttcacga ctggccagga tccccccatg
     1861 gccggcctgc ccgggcctga tcccgggctg tcgcggatgg agtcgagcgt cgtccaggtc
     1921 gtcgcgagcg gtccggacgt tgcgggcgac cctttgtacg agtcgctctt ggccctgata
     1981 ttcggcgcgc gcgaccgcat ctggatcgtc acgccatact atgtccccga tgagatgcta
     2041 gcgagggctc tcaatcttgc ggcccgccgg ggggtcgatg tccgggtgat ggtacccgcg
     2101 cgatccaacc acgtgacggc cgacctggcg cgcgccggct acctgcgcga cctctacgaa
     2161 gccggcggcc gcatcttgct ctataccccg gccatgctcc atgccaaggc cgtcgtcttc
     2221 gacgatgaaa tcgccattat cggttcggcc aatatggaca tgcgcagcct gttcctcaac
     2281 tacgaagtag ccttgtacgt ctcgtcgccg gctcaggtcg gcgaggtggc ggcctgggct
     2341 cgctcgctca tggtggattc acggctcgag ctccccgcgc taggatgggc acgcgagctc
     2401 tccgaaaaca tcgttcggct cctctcaccc cttttataaa atcataaagg tagaatatcg
     2461 ccatggctgc aacggaaagc ctctggtaca aggatgcgat cttctatgag gtttacacgc
     2521 gtggcttttt tgatgcaaat ggcgatggcg acggcgactt gatcgggctg acttccaagc
     2581 tcgactacct tcaagatctg ggcgtcgact gtctgtggct catgccgatt tatgcctcgc
     2641 cgcacaaaga cgacggttac gatattgccg atttccgcca gatccatccc acgatcggca
     2701 ccgtcgccga ttttgaaggc ctgacgaaag ccgcacacgc gcggggcatg cggatcatcg
     2761 ccgacctcgt ggttaaccac acctccgacc agcacccgtg gtttcaggag gccaggcgcg
     2821 acccctcttc cccctatcgc aactactacg tctggagtga caccgaccag gtctacaagg
     2881 gagcgcgcgt catcttcagg gacaccgaaa cctccaactg gacctgggac cctgtcgccc
     2941 gccagtactt ctggcaccgt ttctatagcc atcaacccga cctcaatttc gacaatcccc
     3001 aggtcaagaa agaaatcttg aacgtcatgg cgttctggct cgaccgcggg atcgacggct
     3061 tccgcgttga tgccgttccc tatctgttcg agcgcgaagg caccaactgc gaaaacctgc
     3121 ccgagaccca tgacttcttg aaggagatgc gccagttcgt cgattcgcgc taccccggct
     3181 cattgctctt ggccgaggcc aaccagtggc ccagcgatct attgccttat ttcggccagg
     3241 gcgacgagtt tcacatggcc ttcaactttc cgttaatgcc tcgccttttc atggccatcc
     3301 gccgcgaaga ccagcgcccg ctgatcgaca tcatgcatca aatgcccgat gtccctactt
     3361 cgtgccaatg ggcccttttt ctgcgtaacc acgatgagct gaccctggaa atggtgacgg
     3421 atgaagagcg cgactacatg tacacggaat atgcgcgtga tccacgcatg cgactcaacc
     3481 tggggatccg ccgccggctc gcccctttga tggaaaacgg ccgcaggcag atcgagctct
     3541 tgcacagctt gctgtttagc cttcccggca gcccggtgct ttactatggc gatgagatcg
     3601 gcatgggaga caatgttcat ctcggcgacc gcggaggtgt gcgaacgccc atgcagtgga
     3661 caggggatcg gaacgcgggg ttttcgaggg ccaatccatc ggccctctat caggcgatca
     3721 ttcttgatcc gatttatcac taccaggcgg tgaatgtgga agcgcagctt gcctcgccga
     3781 catcgctttt gaactgggtg cgtcggttga tcaaggtccg ccgcgcacac aaggtctttg
     3841 gccgtggcaa cctcgaattc ataccttgcc acaaccgcca cgccgtcgcc tac
//
LOCUS       DATAQL010001155         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2714621, whole genome shotgun sequence.
ACCESSION   DATAQL010001155 DATAQL010000000
VERSION     DATAQL010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2714621"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..302
                     /locus_tag="VHS97_19965"
     CDS             24..302
                     /locus_tag="VHS97_19965"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450543.1"
                     /translation="MSRIFAIPYDMAFSPDGRHLASGNWDRTARLWDAATGRELVVFR
                     GHDGRVTDVALRPDGRYLASTTAGGTVKIWDVTPWTERPAGEPSAPGR"
     gene            389..442
                     /locus_tag="VHS97_19970"
                     /pseudo
     CDS             389..442
                     /locus_tag="VHS97_19970"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            518..670
                     /locus_tag="VHS97_19975"
                     /pseudo
     CDS             518..670
                     /locus_tag="VHS97_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
     gene            704..940
                     /locus_tag="VHS97_19980"
     CDS             704..940
                     /locus_tag="VHS97_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450544.1"
                     /translation="MRSFLPARVALLARLWAKAVTLAGHGPPVAGSVRLSEGLNLATG
                     PCLGPGRATASPQPLGPGKFRVEPKTKSGRRRGG"
     gene            1669..2904
                     /locus_tag="VHS97_19985"
     CDS             1669..2904
                     /locus_tag="VHS97_19985"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX3450545.1"
                     /translation="MPKPKLFGGNCSPALKLLKLLVAASAISFLLGLLVWQAIRFDGA
                     PDPTTEGLSPRAVVLNTGILVFREGLEAILVLAALTASLSRTNEGYWKPIALGSLLSF
                     GASVATWFVVVGLIDSINAPALHVQAATGLLAIIVLLVIMNWFFHRIYWSGWITHHNR
                     RKRELLSSEGSNRPALFRGLVFLGFTAVYREGFEIVLFLQTMRLRAGNLVVARGVTLG
                     LTLTLIVAVLTFVLHQRLPYKKMLVLTGIMLGSVLLVMVGEQVQEMQQAGWCSTTSVA
                     FSIPDWMGTWFALFPNVEGLASQAAAAIFVIGSYFAARYVSGRPRAFEAKNSQVTLAA
                     GGGHSARAVPVQPVGAELDLDAPFTADRGGVSMTDSCRAHPLAASADGARIELTASTR
                     PGPTFAASARFDHLMDSPA"
     gene            complement(3007..3354)
                     /locus_tag="VHS97_19990"
     CDS             complement(3007..3354)
                     /locus_tag="VHS97_19990"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3450546.1"
                     /translation="MRYLCLYKSAKPEGTPPTQEEMAEMGKLIEESFKSGRLLAAEGC
                     LPSAMGARVRLSQGKYHVTDGPFTESKEVIGGFALIQAKSKEDAIEFIKTFLKIAGDG
                     ESEIRQVFEAPSS"
BASE COUNT          707 a         1113 c         1060 g          777 t
ORIGIN      
        1 aatcgtgaag acaatggccc actttgtcgc gaatctttgc cataccctac gacatggcgt
       61 tcagccccga cggccgccac ctcgcctcgg ggaactggga ccggactgcg cggctctggg
      121 acgcggcgac cgggcgcgag ctggtcgtct tccgcgggca cgatggccgc gtgacggacg
      181 tggccttgcg ccccgacggc cgctacctcg cctcgacgac tgcgggcgga accgtgaaga
      241 tctgggacgt gacgccctgg acggagcggc cggccggcga accgtcggcc ccgggccgct
      301 aacgcatacc cgccgtggaa aggaccaacc gtgtgatcaa ctcccgggcg gccagccgcc
      361 ccgggcccaa tagggaaggg ggaccataat gcatcgtcgc ggcttcacgc tgatcgagct
      421 gctggtggtc atcacgatca tgccgccgaa cggcacgacc tgcgtctatt catgtacacc
      481 gacggccatc cgcccgtcac cggtgcactg gtgcggggcc ttgacggccg gcagccgaca
      541 ccccggcctg gtcaacgtcc tgatggccga cggctcggtc catgtgatca aggcgacggt
      601 gagcaacgga gtctggcggg ccctcgggac ccgcgccggc ggcgaggtca tcgccgcgga
      661 tgcctactga acagaggtga ggctacctta ggcgaccgga gccgtgaggt ccttcctgcc
      721 ggcacgcgtc gccctcctcg cgaggctctg ggccaaggca gtaacgctcg ccggccacgg
      781 cccgccggtg gccgggtcgg tcaggctgtc cgaggggctg aacctagcga ccgggccatg
      841 cctcggcccg ggccgagcga cggccagccc acagccactc ggaccgggaa agttccgggt
      901 cgagccgaag accaagtcgg gacgccgccg cggcggatag gaacgtcttc cccggagtca
      961 tcgctgccat ggtcgccggg acgggatctg gtatttcccc gatccgagga ttacgctcag
     1021 cgaagtgtcc aatcaacccg gggcagtcct cccgaatgaa gtttcagttc ttcacccgcc
     1081 aagtcagaat gcgtcatccc tcaatcccat gagctactgg actcgtttaa ggtttatttc
     1141 ctacttacca aaagcaccag gtccagcgtg gaataaacca ataagcaata gaccgactgc
     1201 agcagcacac cacgccgctt caatgtgctc tccaatgtgg gcccaataga tccaaaatgg
     1261 agtagtgaca atagcaagcc aaccggcgat caggttaggg attggcccat tgcccaatgc
     1321 gcatgggtaa ctctccttgg taattccacg aacgaaatga ggtatcccgt tgccaaacca
     1381 aatacccgcc aacagactga gaataactgt cgtaaacatg tttaatccca ccattcattt
     1441 ctgcatcatc gataagcgat gagaaattgg tctttggagc aagcaatgta gagccctacc
     1501 ggtttcccga atgcgtgccg aagccgctgc ctgcttgggg tttgataacc acagggggag
     1561 tggtttgaga ttgagtctca ttatcattgc cattcttctg gcagcgcgat ataacgttat
     1621 tgacaaaccg tctgtcccga acgatccgag tcgaatcttg ggtaagacat gcccaaacca
     1681 aagctcttcg gtgggaattg ttcccccgca ctcaaactgc tcaagctttt ggtcgcggcg
     1741 agcgccataa gctttctcct ggggcttctc gtctggcagg ccatccgatt cgacggggcc
     1801 cctgatccga ccaccgaggg tctcagtccc agagccgtgg tattgaacac cggcatcctg
     1861 gtcttccgtg aaggtcttga agcaatcctc gtactggcag ctctgacggc tagcctatct
     1921 aggacgaatg aaggttactg gaagccgatt gccttgggct cgctcctgtc gttcggcgcg
     1981 tctgtggcaa catggttcgt tgtcgtgggc ctgatcgatt cgatcaatgc cccggccttg
     2041 cacgtccagg cagcgaccgg ccttctcgct attattgtgt tacttgtgat catgaactgg
     2101 ttcttccata gaatctattg gtcaggctgg atcacgcatc acaatcgccg caaacgagag
     2161 ttgttgtcgt ccgaaggctc aaaccgtcct gcgctatttc gcgggctggt attcctggga
     2221 tttacggctg tttatcgcga aggctttgag atcgtcttgt ttcttcagac gatgcggctt
     2281 cgtgcgggta atcttgttgt cgctagaggg gtgaccctcg gcctcacttt gacgctcatc
     2341 gtcgccgtgt tgacgttcgt acttcaccag cgcctgccat acaagaagat gctcgtactc
     2401 acaggcatta tgctcggaag cgtcttgctc gtgatggttg gcgaacaggt gcaggaaatg
     2461 cagcaagcgg gttggtgctc aacgacgagt gtcgcgtttt ctatcccgga ttggatgggc
     2521 acctggtttg ctctatttcc aaacgttgaa ggacttgcat cgcaggcagc ggcggcgatc
     2581 tttgtcatcg ggtcgtattt tgccgcgcga tacgtaagcg gacgtccaag agccttcgaa
     2641 gccaaaaact cccaggtgac gcttgccgcc gggggcggcc actccgcccg cgcagtgccg
     2701 gttcagccgg tcggggcgga acttgacctg gatgccccat tcacagccga tcggggaggt
     2761 gtcagtatga ctgactcatg tcgggctcac ccactggcgg cttcggcgga tggggctcga
     2821 atcgaactca cggcatccac gcggcccggg cccacgttcg ccgcatccgc tcgattcgac
     2881 cacctcatgg attctccggc gtgatgcgag gcccaccgct cggcctcctt atggcggacc
     2941 cacaccttgg atccccaaag gccggcgccg agagataggc cgacgatcgc gacgtcgcct
     3001 gtttcctcag gagctcgggg cctcgaagac ctggcgaatt tccgattcgc cgtcgcccgc
     3061 gattttgagg aaggtcttga tgaattcgat cgcatcctcc ttcgacttcg cctggatgag
     3121 cgcgaagccg ccgatcactt cctttgactc tgtgaaaggc ccgtcggtca catgatactt
     3181 tccctgggag agcctgacgc gtgcgcccat cgcactcggc agacaaccct cggcagcgag
     3241 cagcctgccg gacttgaagg actcctcgat caatttcccc atctcggcca tttcctcctg
     3301 ggtgggagga gtgccctccg gcttggcgga tttgtacaaa cacaagtatc gcatagtcat
     3361 gcctcctcaa agttggttgg gacttggctc tccgcacggc ttcggccgga catgagacct
     3421 ggctctatcg gtacgtcgac tgaagagaaa ccagatcgac agtggctgga aatttttttt
     3481 acgacacgac cagactgaac tcatgttccc aagcacactc gtgcgagtcg ttgctgttgt
     3541 caaatgcggc ggagctatcg tgacttcgca ttcgggcagt cccccacttc aattcacgcg
     3601 attgtccgat tggtgcaagt tattcccttc aggtcgacgt gattttcttc cgtagac
//
LOCUS       DATAQL010001156         6827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5309020, whole genome shotgun sequence.
ACCESSION   DATAQL010001156 DATAQL010000000
VERSION     DATAQL010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5309020"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1960
                     /locus_tag="VHS97_19995"
     CDS             86..1960
                     /locus_tag="VHS97_19995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3450547.1"
                     /translation="MSQTQGLIGERLGSFRIESILGSGAMGVVYRGTHEKTGREAAVK
                     VVSGDYAPGGKIRDRFEREAEILQQFRHPNIVRFLAVGRFRGTSYIAMEYVQGDTLEK
                     VIQERGHLPWREVVNLAIQMCAALHYAHEHGIVHRDLKPSNLMVTTDGDIKLTDFGIA
                     KDLDATALTATGRTLGTAAYMSPEQIRGTPAVSHKTDLYALGILLYQMLVGKAPFEGS
                     TPVVLMHCHLNEPPPRASAKITEIPKKLDELVVNLMAKLPSDRPWDAAAVGVTLTELR
                     DRAERGDPIAMVWPSTGRDRGVGSAASGAAATPDRPRKKSRKAGATGGTARSTSGSRP
                     ESDSDVRYSWLNRSTIETGLLVLALVVIGGFIAYWVWPPSKEYLYKQADTLMASSRRG
                     DWLTALDDYIKPLDERFPDNPYRDQTRKWRDKILLDEAESRGRNITSGVETSFTRPGN
                     DAERKYVIAHQLAVEARGRGDDLTAINHWRDFAGQVKADDPDERKWHLLALQKIEQVE
                     NAIKDRREYVLKQLQLADAALQSGRPNEGLSIKSKLIEQFSRYTDLADIFGSVPPSNG
                     APAPNTVPGHPTESSPGEKGVAPKQPAPDQSAAAGQANDSPAHSSGDTDQPKAPLHQN
                     "
     gene            2170..2457
                     /locus_tag="VHS97_20000"
     CDS             2170..2457
                     /locus_tag="VHS97_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX3450548.1"
                     /translation="MPQSESRARFIGSRELHRDLPKVLDTLENPNARFVLTIHSKPKA
                     VLIGAEAFLEILRSPRPEDRLLALQLGALVQGLESSSSSAETADLEELVAV"
     gene            2830..6507
                     /gene="dnaE"
                     /locus_tag="VHS97_20005"
     CDS             2830..6507
                     /gene="dnaE"
                     /locus_tag="VHS97_20005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX3450549.1"
                     /translation="MSTPPFVHLHCHSHYSLLDGASKIPDLFERTKSLGMPALALTDH
                     GNLYGAVEFLREAKEAEILPIVGLEAYVAPGKRSERSAGGVSGQEHSFHLTLLAKNAA
                     GFRNLMRLSSLSFLEGFYYKPRIDKEILERHADGIICLSGCASAEFSDYILHNRTAEA
                     ERLCAWYQKVFGEENFYVEIQDNGVGIQKECAVGALDIARRMGLPVVATSDAHYLTRD
                     DAPAHDVLLCIHTGKTVDDSTRMKFETDEFYLRSPDDMYAAMPDHAEALATSARIAEM
                     VEPHYESLGLGRRCFPSFEPPDQKTPEEYLRELCQTGLHERYGEIPPPEASDRLEHEL
                     SIINRMGFASYFLIVWDFVRYAHEKGIPALARGSACGALVSYLLRLSNVCPIKYDLLF
                     ERFLDPSRSEAPDIDIDLCQARRYEVIEYVRKKYGDANVAQIGTFGTLKAKAALKDVG
                     RALNIPLARIEQINKLVPGRLNITLDEAIKEESALRRMADEDAEIAKLLDFARRLEGM
                     NRSVGTHAAGVVIADRPLEELVPLQKLPNKDKAKEVVTTQWEMGDIEKAGLLKMDFLG
                     LRNLTTTEAAVRLIKERHPDNPIDLDNLPLGDPKTFELLQRGETKGVFQLESAGIRDL
                     LIKMKPDRFADIIATNALYRPGPLNGGMVDEYVDVKNGRRHASYLHPVLKEVLEETYG
                     VMVYQEQVMRILNRLGGIELSQAYATIKAISKKKAEFINQSRDQFIQGANSQGLDRDK
                     SAKIFDLIVHFGGYGFNKSHSTAYALVAYQTAYLKAHFPTEFMAAVLSSEMDGAERDK
                     FFVEHIEDCRRMGIEVLLPNINEGHVDFRVASEGKITFGLGAIKGVGFKAVEAIVKAR
                     EQKGPFQSLDDFFERVSAKDVGAGCAETLIRAGAFDCLDARKPNLLRNQLLCVLPRAI
                     QAGQARQDDRRRGQRGLFDQLETPAHNGHSNGAATTLSNLPDVAELSDAELLADEKKV
                     LGFYMSSHPLARHAGLLQALASHRVAELTGLPDKTEVILGGIIANVQAKTVQKSRSGL
                     TRMAKLSFEDLSGTTPAMLWPEEFAKMGDLVKNDLIGWVKGTLDRRRDPPELIVSRII
                     PLENGPAELTRGVVVRLHKGLHQTGHLERLLRTVRIRPGSLDLFLEVVGLELVRRAVY
                     KAGASLRIRYDDRLIPELEAVVGAGQVRLLGQRGATTRVEASNGSADSSLAQSKTTTS
                     VRPPSTATDLDESADSDSDDF"
BASE COUNT         1404 a         2098 c         2052 g         1273 t
ORIGIN      
        1 catggactac gtgcagcaag gttatctgcc ggcggccggc ggcttatcct gttcgatgcc
       61 ccgatcttga tgtgtgatcg cgatcatgag ccagactcaa ggtttgatcg gcgaaaggct
      121 cggctcattt cgcatcgagt cgatactcgg ctccggtgcc atgggggttg tctaccgcgg
      181 gacgcacgaa aagaccggac gcgaggccgc agtcaaggtc gtgagcggcg actatgctcc
      241 cggcgggaag attcgcgacc ggttcgagcg tgaggctgaa atcctccagc agttccgtca
      301 tcccaacatc gtccggttcc tggcggtggg ccgcttccgc ggcacttcct acatcgcgat
      361 ggagtacgtg cagggggata cactcgaaaa agtcattcag gagcgtggcc acttgccatg
      421 gcgcgaggtg gtcaacctgg caatccagat gtgtgcggca cttcattacg cgcacgaaca
      481 cggaattgtc caccgcgatt tgaaaccgtc caacttgatg gtgacgaccg atggcgatat
      541 caagctgacc gattttggga tcgccaagga tctggacgcg acggcgctga cggccaccgg
      601 acgaacgctg ggaacggccg cttacatgtc gcccgaacag atcaggggca ctccggcggt
      661 aagccacaag accgacctgt acgcgcttgg aatccttttg taccagatgc tggtgggcaa
      721 ggctcctttc gagggaagca cgcccgtcgt cttgatgcac tgccacttga acgagccgcc
      781 tccgcgcgcc agcgccaaga tcacggagat tcccaaaaag ctggacgagc tggtcgtgaa
      841 tctgatggcc aagttgccca gcgaccggcc ctgggacgcc gccgccgtcg gcgtcacgct
      901 cacagaattg cgcgacaggg ctgagcgtgg cgacccgatc gccatggtct ggccctcgac
      961 cggccgcgac cgtggcgtgg ggagcgccgc cagcggggcc gccgccacgc ccgatcggcc
     1021 gcgcaagaaa tcacgcaaag ccggcgcgac cgggggcacc gcacgctcga ccagcggcag
     1081 ccgcccagaa tcagactccg atgtacggta ttcctggctc aaccgttcca cgatcgaaac
     1141 cgggctcctc gtattggcgt tggtcgtcat cggcggcttc atcgcctact gggtttggcc
     1201 ccccagtaaa gagtacctgt ataagcaggc cgacacgctc atggcctcga gccgacgcgg
     1261 cgactggctg acggccttgg acgattacat caaacctctc gacgaacgct ttccggacaa
     1321 cccctatcgc gaccaaaccc gaaaatggcg cgacaagatc ttgctcgacg aggccgagag
     1381 ccgaggacga aacatcacaa gcggcgtcga gaccagcttc acccgaccgg gtaatgatgc
     1441 cgaacggaaa tatgtgattg cccaccagct tgcagtcgag gcccgcggcc ggggcgatga
     1501 cttgaccgcc atcaaccact ggcgcgactt cgccggccag gtcaaagccg atgacccgga
     1561 cgagcggaaa tggcacctgc tggctttgca aaagatcgag caggtcgaga atgcgatcaa
     1621 agaccgtcgc gaatacgtat tgaagcagtt gcagcttgcg gacgctgctc tccagtcagg
     1681 ccgtcccaac gaggggctat cgatcaaaag caagctgatc gaacagttca gccgctacac
     1741 cgatctcgcc gatatttttg gctccgtgcc gccgtcaaac ggcgcgcccg ccccgaacac
     1801 ggtccctggt caccccacgg agtcgagccc gggcgaaaag ggcgttgcac cgaaacagcc
     1861 ggcgccggac caatcggcgg ccgccggcca agccaacgat tccccagcgc actcgtcggg
     1921 cgacactgac cagcccaaag cccctcttca ccaaaactaa atcactcggc gcaactcccg
     1981 gtgaccccgg tcgtttcgaa atcgcccaac gcttttgctc cgaattccgt agttagatgt
     2041 taaacactga ttgacgacgc gttggaagtt agctaacttg attgtgccta tcgtacatag
     2101 aggcggctcc cgtctgttcg cctcgatttt tcccatgttc tcggccgatc ttggcccccg
     2161 aggaatctga tgcctcagtc tgagtctcgc gctcgcttca tcggcagtcg tgaactacac
     2221 cgtgatctgc ccaaggtgct cgacaccctt gaaaacccca atgcccgatt tgtcctcacg
     2281 attcatagta agcccaaagc cgtgctcatc ggtgccgagg cattcctgga aattctgaga
     2341 agtccgcgac ccgaagatcg cctgctggcc ctgcagttgg gcgccttggt ccagggcctc
     2401 gaatcgtcga gcagctcggc cgaaaccgcc gacctcgagg agctggtcgc ggtctagcac
     2461 gggtccgccg tgccgagcca gttcgctcag ctcaaaccga aagatagatg ccccccgatc
     2521 cagttggttg cggggggttc gtatttgtgc aaaccatcat gtcatgattg cattgcgtca
     2581 tgatttcggt taaggtgaac aggcgatctc aatcgtggcg ctggggagat tgagtgcccg
     2641 gttgggctgc cggtcccatc ggaatctggc cggctgagat cctagggcgg caagagtccg
     2701 atatggtaga ctcggctggt cgcgtgcagc ctgaggtcgc tcgcgaccgg gacgatcgga
     2761 tcgccccgca tccacggaat gggtccgggc gccggaggcg cgcgggcagc cgcacccgag
     2821 tgaataaaga tgtccacccc gcccttcgtc catctccatt gccatagtca ctacagcctt
     2881 cttgacgggg ccagcaagat cccggatcta ttcgagcgca ccaagtcgct cggcatgccc
     2941 gcactcgcgc tgacggatca tggcaacctt tacggtgctg tcgagtttct ccgcgaggcc
     3001 aaggaggcgg aaatcttgcc gatcgtgggg ttggaggcct atgtcgcccc cggaaagcga
     3061 tccgagcgga gcgcgggcgg ggtgtcgggc caggagcact cgttccacct gactctgctg
     3121 gccaaaaatg ccgccggctt tcgcaacttg atgcggctgt cgtcgctctc gtttctggaa
     3181 ggattttatt acaagccgcg tatcgacaag gaaatccttg agcggcatgc cgatgggatc
     3241 atctgtttat caggctgcgc ttcggccgaa ttttccgact acatccttca taacagaacg
     3301 gccgaggccg agcgcctctg cgcgtggtat cagaaagtct tcggcgaaga gaacttttac
     3361 gtcgagatcc aggacaatgg tgtcgggatt cagaaggaat gcgccgtcgg agccctcgac
     3421 atcgcgcgcc gcatgggtct gcccgtggtg gcaaccagcg acgcccatta cctgactcgt
     3481 gacgacgcgc ccgcgcacga cgttttgctg tgtatccaca ctggtaagac ggtggacgac
     3541 agcacgcgga tgaaattcga gacggacgag ttttatctac gcagtccgga tgacatgtac
     3601 gccgccatgc ccgaccacgc agaagccctg gccacctcgg cccggatcgc ggaaatggtc
     3661 gagccgcact acgaaagctt gggtctgggc cggcgctgct ttccttcatt tgaacccccc
     3721 gaccagaaga cacccgaaga gtatctgcgt gagctttgcc agaccggcct tcacgagcgc
     3781 tacggcgaaa tcccaccgcc cgaggcgtcc gaccggctcg agcatgagct gtcgatcatc
     3841 aatcggatgg gctttgcatc ctatttcctg atcgtatggg acttcgtgcg ctacgcgcat
     3901 gagaagggca ttcccgcgtt ggcccgcgga tctgcgtgcg gcgccttggt cagctatctg
     3961 ctccgtctga gcaacgtctg cccgatcaag tacgaccttt tgttcgagcg atttctcgac
     4021 cccagtcgct cggaggcacc cgacatcgac atcgacctct gccaggcgcg gcggtacgag
     4081 gtcatcgaat acgttcgcaa gaagtatggg gacgccaatg tggcccagat cggcaccttc
     4141 ggcaccttga aagccaaggc cgccctcaag gacgtggggc gggctttgaa cattccgcta
     4201 gcgcgcatcg agcagatcaa taagctggtg ccgggtcgtc tcaacatcac tctcgacgag
     4261 gccatcaagg aagaatcggc cttgcggcgg atggccgacg aagacgctga aattgccaag
     4321 cttctggatt tcgcccgtcg cctggaagga atgaaccgga gcgtcggcac tcacgccgcc
     4381 ggcgtggtga tcgccgaccg gccgctggaa gagctcgtcc cgctccagaa gcttcccaac
     4441 aaagacaaag ccaaggaggt tgtcaccacc cagtgggaga tgggggacat cgagaaagcc
     4501 gggctcctga agatggactt tctgggcctg cgcaacctga caacgaccga ggcggccgtc
     4561 cggctcatca aagagcgcca tcccgacaac ccgattgacc tcgacaattt gcctttgggc
     4621 gatcccaaaa cctttgagct cttgcagcgc ggcgagacca agggagtctt ccagctcgaa
     4681 tcggcgggaa tccgcgacct cctgatcaag atgaaacccg atcgattcgc cgacatcatc
     4741 gccaccaatg ccctctatcg ccccggccct ttgaacggcg gcatggtcga tgaatacgtc
     4801 gatgtcaaaa acggccgccg ccatgcatcc tacctgcatc ccgttcttaa agaggtcctg
     4861 gaagaaactt acggggtgat ggtctatcaa gagcaggtca tgcggatctt gaatcggctg
     4921 ggaggcatcg agctctcgca ggcgtacgcg accatcaagg cgatctcgaa aaagaaggcc
     4981 gagtttatca accagagccg cgaccagttt atccagggag cgaattcgca ggggctggac
     5041 cgtgacaagt cggccaagat cttcgacctg atcgtgcatt tcggcggcta cggctttaac
     5101 aagtcgcata gcacggcgta cgcgctcgtc gcctaccaga ccgcttatct gaaagcccac
     5161 ttcccgaccg aattcatggc ggccgtgctt tcttcagaaa tggacggggc cgagcgagac
     5221 aagttttttg ttgaacatat cgaagactgc cgacgaatgg ggatcgaggt cctcttaccc
     5281 aatatcaacg aggggcatgt cgatttccgc gtcgccagcg agggaaagat cactttcggc
     5341 ttgggcgcga tcaagggagt tggtttcaag gcggtcgagg cgatcgtcaa ggcgcgcgag
     5401 cagaaaggcc cgttccagag cctggacgac ttttttgaga gggtgtcggc caaggatgtg
     5461 ggcgccggct gcgcggagac gctgatccgg gcgggggctt ttgattgcct cgatgcccgc
     5521 aaaccgaatt tgctgcgcaa tcagctcctt tgcgtgttgc ctcgtgccat tcaggcgggc
     5581 caggcccggc aagacgatcg ccggcgcggc cagcgcgggc tgttcgacca gctcgaaacg
     5641 cccgctcaca atggccattc caacggtgcg gccacaaccc tgtcaaacct gcccgatgtc
     5701 gccgaattgt ccgacgccga gctcttggcc gacgagaaaa aggtgctggg cttttatatg
     5761 tccagccatc cgctcgcgcg gcacgcgggg ttactccagg cgctggcgag ccaccgcgtt
     5821 gccgagctga ccgggctgcc cgacaagacg gaggtgatcc tgggagggat catcgccaac
     5881 gttcaggcca aaaccgtgca gaagagccgg tcggggctca cgcggatggc caagcttagc
     5941 tttgaggact tgagcggcac aacgcccgcg atgctctggc ccgaagagtt cgccaaaatg
     6001 ggcgacctgg tcaagaacga cctgatcggc tgggtaaagg ggactctcga ccgccgccgc
     6061 gacccccccg agctgattgt tagccggatt atcccgctcg agaacgggcc ggccgagctg
     6121 acgcgcggag tggtcgtacg gctgcacaag gggctgcacc agaccgggca cctggagcgc
     6181 ctgttgcgca ccgtgcggat tcgccccgga agcctcgacc tcttcctcga ggtcgtggga
     6241 ttggagttgg tgcggcgcgc ggtctacaaa gcgggggcat cactgcgcat ccgctacgac
     6301 gaccggctga tccccgaact ggaggccgtt gtcggagcag ggcaggtgcg cctgctaggc
     6361 cagcgcggcg cgaccacgcg ggtcgaggcc tcaaacggtt cggcggacag ctctctcgct
     6421 caatcgaaaa cgactaccag cgtgcggccg ccgagcaccg caaccgatct cgatgagtcg
     6481 gccgacagtg actccgacga cttctgaacc attcggccgt ggcggccgaa gaactcacgt
     6541 gctgaggccc cgtcagcaag gtgtggtggg ggccgcggcg ggcgaccgcg cccaacaacc
     6601 taattccttg aaagtggacg gcttatgcag tcgtcgctgt catctgagcg ctagtttcac
     6661 gcaatataca cgaattcttc actaaactat aacacttttg ggagggcgac tagctactat
     6721 tctaacctat gtcatgccaa agcatgaggc tgggtcgtgc tgccaaaact gatcttagtt
     6781 tttagctgga caccgcgtca gtcatttcca actctacgtg acctggg
//
LOCUS       DATAQL010001157         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_576533, whole genome shotgun sequence.
ACCESSION   DATAQL010001157 DATAQL010000000
VERSION     DATAQL010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576533"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VHS97_20010"
     CDS             <1..630
                     /locus_tag="VHS97_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450550.1"
                     /translation="HWLKDASLRAEGQSAERIRDLLEERAATRVVERMERIGELYLGG
                     QVSAARRQANELSKVLELLARQLEILHRTIVAPELTAMVDLDRRVAELTAKLKTITTD
                     AEIAGWRHLAANLVRDLEKAGLTGAATALVNAFEAGGWHWGVGNEHFRTAPAGFTIAL
                     QSVSSLLQAKIQNLILKDMASARDEATPPQFKELVERYYEVLSKEIGKE"
     gene            692..>2297
                     /locus_tag="VHS97_20015"
     CDS             692..>2297
                     /locus_tag="VHS97_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450551.1"
                     /translation="MNVKSTIDWATPYSLSLLLAAAVAAVAIFALLRWASGRPIAPAR
                     RPGLLVLRLAILSIVALIIVNPVRVDLSPGIVELPKLFYLLDTSQSMAIGKDMTRWEQ
                     VVRMIRDADGTRGSDNGAQVSMFRFGSRLAALDAACLRPAGAEPALRSTAGAVLAAEP
                     PRAADPSPAPTDSDTLLGASLESLTDRFGQAPPQAVVVFSDGRARDPDRADAIARAYG
                     VMKVPVHVLPVGDEHVGGDVAIVSVVAPNQVRKFSKVAAQVYVRSYGYKGKRSELKIV
                     TGESQGKPQTVLARIPIVLQDGVAGYSLVFESGDQDRHIEARIDPQPDEVSALNNAFG
                     ADLAIDHTKIRVLYIEGATDSYAAPRRQAGFQGMEVRGAYSSLQEALMEDPDVECTAI
                     IPGGAGGDFSTVIRPNENGRGLPETPSEWFAYDALILSNVPRDALSDQQIAWVEEWVG
                     RRGGGLCMAGGPNSFGSGRWNDTSIGTMLPVQLIPAAHDWDLAQTTIRPVTDGAVHPI
                     LHLTSDEAQNTSLLTTLPNFQGNNHVG"
BASE COUNT          418 a          717 c          747 g          415 t
ORIGIN      
        1 cattggctga aagatgcgag cttacgcgcc gagggacagt cggccgagcg gatccgcgat
       61 ttgctcgagg aacgggccgc tacccgggtt gtcgagcgca tggagcgaat tggcgagctc
      121 tacttgggtg gccaggtgtc ggcggctcga cgccaggcaa acgagctatc gaaggtgctc
      181 gagttgctcg cgcgtcagct tgaaatcctg caccgcacca tcgtcgctcc ggagctcacc
      241 gcgatggtcg atcttgacag acgcgtcgcc gagctgacag cgaagctcaa gaccatcacg
      301 accgacgcgg agatcgccgg gtggcgtcac ctggctgcca atttggtgcg cgaccttgag
      361 aaggccgggc tgacgggcgc cgcgacggcg ctggtcaacg cattcgaagc gggcggctgg
      421 cattggggcg tcggcaatga acacttccgg accgctcctg caggtttcac gatcgcgctt
      481 cagtccgtaa gcagcctgct gcaagccaag atccagaacc tgatcttaaa agacatggcg
      541 tcggcccgcg atgaggcgac gcctccccag ttcaaggagt tggtcgagcg ttattacgag
      601 gtgctttcca aagaaattgg taaggaataa tgcggactga actgcaatct ggttgcggct
      661 ccgccgcatc aagtctcatg agcggggatg catgaacgtc aagtcgacaa tagattgggc
      721 gacaccctat tccctcagcc tgttgctggc agcggcggtg gcggcggtgg ccatcttcgc
      781 gctcttgcgc tgggcctcgg gccggccgat cgcgcccgcc cggcgaccag gactcttggt
      841 cttgcggctg gcaatcctgt cgatcgtcgc tctaattatc gtcaaccccg tgcgggtcga
      901 tctatcgcca gggattgtag agctgccgaa actgttttac cttctcgaca cttcgcagag
      961 catggcgatc ggcaaggaca tgacccgctg ggagcaggtc gtgcgaatga ttcgtgacgc
     1021 cgatggtacc cggggctctg acaacggggc ccaagtgagt atgtttcgat tcggcagtcg
     1081 gctggcggcg ctggacgcgg cctgcctgcg gccggcggga gccgagcccg cgctccggag
     1141 cacggccggt gcagtcctcg ccgcagagcc ccctcgcgcc gccgacccgt cgccggcgcc
     1201 aacggattcc gacacgctat tgggtgcctc gctcgagagt ctcaccgacc gcttcggcca
     1261 ggcaccgcct caggcggtgg tcgtcttctc cgatggccgc gcccgcgacc ccgatcgggc
     1321 cgacgcgatc gcacgagcct acggcgtcat gaaggtgccc gtccatgtct tgccggttgg
     1381 tgacgagcac gtcggcggcg acgtggccat cgtgagcgtg gtcgcaccga atcaagtccg
     1441 gaaattctcg aaagtggccg cgcaggtcta cgtgcggagc tacggctata agggcaaacg
     1501 ctccgagctc aagatcgtga cgggcgagtc ccaaggcaag ccgcaaaccg tcctcgcccg
     1561 cataccgatc gtccttcagg atggcgtggc cggctactca ctcgtttttg agtcgggcga
     1621 ccaggaccgg catatcgagg cgcggatcga tccgcagccg gacgaggtgt cggcgctcaa
     1681 caacgctttc ggagccgacc tggccatcga ccataccaag atccgtgtgc tctacatcga
     1741 aggggcgaca gacagctacg ccgcaccgcg acgacaggcg gggtttcagg gaatggaggt
     1801 tcgcggggcc tactcatcgc tgcaggaagc gcttatggaa gatccggatg tcgagtgcac
     1861 ggcgattatc ccgggcgggg ctggtggcga cttctcgacc gtgattcggc ccaacgagaa
     1921 cgggcgcggg ctccccgaga ctccatcaga atggttcgcc tacgacgcac tgattttgag
     1981 caacgtccct cgggatgcct tgagtgatca gcaaatcgcc tgggtcgagg agtgggtcgg
     2041 ccggcgcggg ggcgggctgt gcatggccgg cggtcccaac agtttcggtt ccggccgttg
     2101 gaatgacacc tcgatcggca cgatgctccc cgtgcagctc atcccggccg cccacgactg
     2161 ggatctggca cagacgacga tccgcccggt taccgatggc gccgtccacc ccatcttgca
     2221 tctcacgtcc gatgaggcgc aaaacacctc gttgctgact acgttgccca acttccaggg
     2281 aaacaatcat gtcggtc
//
LOCUS       DATAQL010001158         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1492187, whole genome shotgun sequence.
ACCESSION   DATAQL010001158 DATAQL010000000
VERSION     DATAQL010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492187"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VHS97_20020"
     CDS             complement(<1..1471)
                     /locus_tag="VHS97_20020"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX3450552.1"
                     /translation="MITDFIVLVSLALPTQNPPPENPPSATVGPRQGALVIVGGGQVS
                     PDIVKQFVTLAGGKDSEVVVIPTAAENDPVDLKRVGEGFTRSFGFKNVTVLHTRDRAE
                     ADSEGFVAPLRKARAVWFNGGRQWRIVDSFLGTRTQREIEAVLERGGVIGGSSAGATI
                     QGSYLVRGARDGNDVLMAKGYEQGFGYLKGVAIDQHILPRGRADDLVQVIGAHPELLG
                     LGIDESTAVVVEGDRFEVIGRGVVGIYDGKNHDGKRYYFLAPGEHFDLKRRQRLSSEA
                     IRPSRAGTSPASPGQPTPTGARPLGDQTSSPRTGSASQWAGAQAPPATAVRSVTTPKQ
                     FFGFHIGDDYSLANYQQLKGYWEKLERESDRIKVVNIGATEEGRPQLVAIVTSAANHR
                     RLDRYQQIARRLALSDGLDRTEAHKLAVEGKAVVWIDAGIHATETLWPQAMIETVFQL
                     VAGTDDETLRLLDDVIILFVHANPDGHDLVADWYMREKDP"
     gene            complement(1504..2691)
                     /locus_tag="VHS97_20025"
     CDS             complement(1504..2691)
                     /locus_tag="VHS97_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3450553.1"
                     /translation="MTFSIMRNRPEPALAGFPRQTVLVSSTSADRSNPLPRHRAGFTL
                     IELLVVIAIIAVLIALLLPAVQAAREAARRIQCTNNLKQIGLALHNYHSTHDVFPPLA
                     IHVNGVAGPTDYNDQNGPSVLLFALGNLEGQNVYNAFNFQIGAVQDNVVGNTPQNTTV
                     TNTTINTFICPSNSYTSVYPHSTNYAASYGPQFRWDATSGGIGVGLFAANLARGLRDI
                     TDGSSNTIAFAEVNTGDGAVGSRNHTEIFRYVPWPGSTESGNGLEQVATNPLGYANWL
                     TYMQKCDAYRGSAGAAANELDQASQYWALSRCHRGAVTSLLHTPNNPHEDCMQISQYD
                     PEQYPTVGPNAAAASRSWHPGGVNVLLGDGSVRFIKDAISQQTWWALGTKSGGEIISA
                     DSY"
     gene            complement(2926..4011)
                     /locus_tag="VHS97_20030"
     CDS             complement(2926..4011)
                     /locus_tag="VHS97_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3450554.1"
                     /translation="MNRGLSAKNRRRGFTLIELLVVFAIIAVLIALLLPAVQSAREAA
                     RRAQCVNNLKQLSLAAANYESSNTCLPAGEFVQHSSLDPTQWKYGGGSAYVRLAQFLE
                     QGAAYNSWNASVTTFSPPNWTLATVGLSTLWCPSDPKATDRYSLATIYAGNGTTMDGN
                     FAPDGLLQAYTSYVVNNGTWYMPSYVTPDTAYSAQYNAYRQATNGVIYSGSSVRFAEI
                     TDGLSNTVAFSERSRGIFGPDDLTYDQWWNSGDYADHGFATRYPINAYRTFATQIANG
                     GGRILYTSVASFHPGGANFGFLDGSVRFLKETINTWQLDNSTPYPLPIGIAYGSFGEP
                     LMGTSRPGIYQALSTRNGGEVTSADGY"
BASE COUNT          914 a         1350 c         1526 g         1026 t
ORIGIN      
        1 cggggtcttt ctcccgcata taccagtccg cgacgaggtc gtggccgtcg gggttggcgt
       61 ggacgaatag gataatcacg tcgtcaagga ggcggagcgt ctcgtcgtcg gtcccggcga
      121 ccaactggaa gacagtctcg atcatcgcct gcggccaaag tgtctcagtg gcgtggatgc
      181 cggcgtcgat ccacaccacc gccttgccct cgaccgccag cttatgggcc tcagttcggt
      241 ccagtccgtc ggagagggcc aggcggcgcg cgatctgctg atatcggtcc agccgccggt
      301 ggttggcggc ggaggtaaca atggccacga gttgtggccg gccttcctcc gtcgcaccga
      361 tattgaccac cttgatccgg tctgactcgc gctcgagttt ttcccagtaa cctttcaact
      421 gttgataatt cgccaggcta tagtcgtcac cgatatggaa gccgaagaat tgttttggcg
      481 tcgtgacaga gcgaacggcc gtcgcgggtg gtgcttgcgc gccggcccat tgtgacgcgc
      541 tgccggtacg cggagaagac gtttgatcgc ccaggggtcg ggcgcctgta ggagttggtt
      601 gcccgggcga ggctgggctt gtgccggcgc gcgagggacg aattgcttcc gacgagagtc
      661 tctgacgtcg cttgaggtcg aaatgctcgc ccggggcgag gaaataataa cgcttgccgt
      721 catggttctt gccatcgtag atcccgacca cgccgcggcc gatcacctcg aatcggtctc
      781 cctcgacgac gacagccgtc gactcgtcga tcccgaggcc gagcagctct gggtgggcgc
      841 cgatgacctg gacaaggtca tcagcccggc ccctggggag aatatgctga tcgatggcca
      901 cgcccttaag gtagccgaag ccttgctcgt agcccttggc catcaaaacg tcgttaccat
      961 cgcgggcgcc gcgcaccagg taagagccct ggatcgtggc gccggcggag ctgccgccga
     1021 tcacaccgcc gcgctcgagg acggcctcga tctcgcgctg ggtgcgcgtc cccaaaaagg
     1081 agtcgacgat ccgccattgc cggccgccat tgaaccagac cgcccgggct tttcgtagcg
     1141 gagccacaaa tccttccgag tcggcctcgg cacggtcacg ggtgtgcagg accgtcacgt
     1201 ttttgaaacc gaacgacctg gtaaagcctt cgccgacccg cttgagatcg accgggtcgt
     1261 tctccgccgc cgtgggaatc accacgacct cggagtcttt gccgccggca agcgttacga
     1321 actgtttcac aatgtcggga ctgacttgac cgccgccgac gatcacgagc gcaccctgcc
     1381 gcggcccgac agttgccgag ggtgggttct ccggcggagg gttctgggtg ggcaaggcca
     1441 gcgacacgag cacgatgaag tcagtaatca tgcaagcacc ctctaagcta aatgtgcgat
     1501 ttatcagtac gagtcggccg atattatctc accgccggac ttggttccaa gcgcccacca
     1561 agtttgttgt gagatagcgt ccttgatgaa acggacactc ccgtcgccca agaggacgtt
     1621 gacgccgcct gggtgccagc tccggctcgc cgctgctgcg ttgggcccga ccgtgggata
     1681 ttgttctggg tcatattggg atatttgcat gcagtcttcg tgcggattgt tcggggtatg
     1741 taatagcgaa gtcacagccc cgcggtggca acgggagaga gcccaatact ggcttgcctg
     1801 atcgagctcg ttggccgcag cgccggccga accacggtag gcgtcacact tctgcatata
     1861 ggtcaaccag ttggcgtaac ccaaagggtt ggtcgccacc tgctcgagcc cgttgccgct
     1921 ctcagtcgag cctggccagg gaacgtaccg gaagatctct gtatggttgc ggcttcccac
     1981 cgcgccatca ccggtgttca cttcggcgaa ggcgatcgtg ttgctggacc cgtcggtaat
     2041 gtccctgagc ccccgcgcca ggttggcggc gaatagcccc acaccgatcc ctcccgacgt
     2101 tgcgtcccag cgaaactggg ggccgtagct ggccgcatag ttggtcgaat gcgggtacac
     2161 gcttgtgtag gagtttgatg ggcagatgaa ggtgtttatg gtggtattcg tgacggtggt
     2221 gttttgtggt gtgttgccca ccacgttatc ctgaaccgcg ccgatctgaa agttgaacgc
     2281 attgtagacg ttttggcctt cgaggtttcc aagggcgaac aggagtacgc tcgggccatt
     2341 ctggtcgttg taatcggtag gcccggcgac gccgtttacg tggatggcaa ggggaggaaa
     2401 gacgtcgtgc gtcgagtggt agttatggag tgcaaggccg atttgcttga ggttattcgt
     2461 gcactgaata cgccgggccg cctcgcgcgc tgcctgaacg gccgggagta acaaggcgat
     2521 caggaccgcg atgattgcga tcaccaccag caattcgatg agagtaaagc cggcgcgatg
     2581 gcgaggaagc ggattggatc gatccgcaga ggttgaagaa acgagcactg tttgtcgagg
     2641 gaatccagcc aaggccggct cgggccgatt gcgcataatt gaaaaggtca tgtgagagtc
     2701 catcgatttc gcccgcttcc ggcctgtccg gtcagcgatg tgcggtcatt ccgatcgaag
     2761 aacctctcga tgacgagtcg gcgacgtgag aaacagatgg ttttgccggt tcaattgact
     2821 gcgccaaccg ggagccggtc ggcctgagtg agtgggcgag cgggctcgaa cttcagtgcc
     2881 ttgctcggct gattaggcac ttgtcgtcgg ccggtgtctg tttggtcaat agccgtcggc
     2941 gctcgtcact tcgccgccgt ttcgggtcga gagcgcctgg tagattcctg gtcggctcgt
     3001 gcccattaag ggctcgccaa agctgccgta ggcaattccg atcggcaacg ggtatggcgt
     3061 gctgttgtca agctgccagg tattgatagt ttccttgagg aatcgaaccg agccatcaag
     3121 aaatccgaag ttcgccccgc ccgggtggaa gctggcaaca gaggtgtaga gaatacgtcc
     3181 gcccccgttg gcaatttgtg tcgcgaacgt tctataggcg ttaattgggt accgggttgc
     3241 gaatccgtgg tcggcgtaat cacccgaatt ccaccactga tcgtaggtga ggtcgtcagg
     3301 tccaaatatg ccccgcgaac gttcactgaa ggcgaccgtg ttcgagagcc catcagtgat
     3361 ttccgcgaag cggacgctgc ttccggaata gatcactccg ttggtggctt ggcgatacgc
     3421 gttgtactgc gcactgtaag cggtgtccgg agtgacgtac gagggcatgt accacgttcc
     3481 gttgttaacg acgtaactgg tgtacgcttg gagcaggcca tcgggagcga aattgccatc
     3541 catagtcgtt ccgttgccgg cataaatagt ggcgagcgag tagcgatcgg tcgcctttgg
     3601 gtcgctgggg caccagagcg tgcttagccc cacagtggcc aaagtccagt tggggggtga
     3661 gaaagtcgtc accgacgcat tccaggagtt gtaggcggct ccctgttcca ggaattgggc
     3721 gagccgcacg tacgcactgc cgccgccgta tttccattgc gtcggatcga gtgatgaatg
     3781 ctgcacaaac tcgcccgcgg gcaggcaggt gttcgatgac tcatagtttg ctgccgccag
     3841 tgaaagctgc ttgagattat tgacgcattg ggcgcgccgg gccgcctcac gcgccgattg
     3901 aacggcggga agcaagagcg cgatcagaac cgcaatgatt gcaaaaacga caagtaattc
     3961 gataagcgta aagccgcgcc ggcggttttt ggcggatagt ccgcgattca tgatttagca
     4021 acctctgtct gtctttttgg gataatgaaa cgagggtcgt tcaggacctg ggctcgcgtt
     4081 gtacgccgcg aggtcgtccg tcgggcgctg ccgccctttt ggctgccttg cctgtcaggc
     4141 cagttggtat cggcgggatg ttcgattcct tggtcggcga gacgaccgca gtgccttggc
     4201 cgtcgggagc gcaaccgttt accgcgagcg aaacgcctat cagggcgggg acgagtaagc
     4261 gacggccacc gattcgccgg acgttgatgg tcaccttgtt gcctgacctc acacgactcg
     4321 ggaagcgcgg ccgccggcca ggcagacgac acacttgaca acccgagcgg ggaaactccc
     4381 cgggtgactt gaactgcggc gcactcccca atgaagcgtg ccgtatgtcg gacagctcac
     4441 ccaagatgcc tctgggcgag gtcgggtatt ttacccgttc gcctaatcat acgacaaacc
     4501 gtctgcttgt aaagatcaag tcgatcgaat tagtagactt tgtgattgtt gttgaaagct
     4561 attgtatgca aagtgatttc tagccattga cttgcggcaa cgaattttcg gcgagtcgga
     4621 cgaatctcaa gggactgtcc ggttccgcaa ggtctgtgac attcgccccg ttctggtcgc
     4681 tcggctcgct caacagaggc gagatcatct cttctgactc gtactgagtg ccgtccgccg
     4741 atcgatacct cgcgcaggcg gaatcgattc attttgggag tgcgaggctg gcgtggtgaa
     4801 aagttgatct tgactg
//
LOCUS       DATAQL010001159         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2410310, whole genome shotgun sequence.
ACCESSION   DATAQL010001159 DATAQL010000000
VERSION     DATAQL010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410310"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..859)
                     /locus_tag="VHS97_20035"
     CDS             complement(65..859)
                     /locus_tag="VHS97_20035"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX3450555.1"
                     /translation="MRQYLDLIERILADGTEKHDRTGTGTLSVFGHQMRFDLAQGFPL
                     TTTKKLHLRSIVHELLWFLAGDTNVKYLNEHGVTIWDDWADESGELGPVYGRQWRSWP
                     TPDGGVIDQIADVVAAIRRNPDSRRLIVTAWNPADVDKMALPPCHCLFQFYVATGRLS
                     CQLYQRSADVFLGVPFNIASYALLTLMVAQVTGLKPGEFVHTLGDAHLYRNHLEQAQL
                     QLSRVPRSLPRMTLNPAVRDLFAFHYEDFSLHGYDPYPHIKAKVAV"
     gene            complement(960..>1818)
                     /locus_tag="VHS97_20040"
     CDS             complement(960..>1818)
                     /locus_tag="VHS97_20040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3450556.1"
                     /translation="GPTDVISRIISDHMARTLGQSIVIENTLGAGGTTASTRAARATP
                     DGYTIEMGHMGTHAASVALYPNLAYKPDVDFAPIGMVAGTPVLILAKKDFPPNDLREF
                     VTYVKANVASLNMAHAGVGSVSFTTCLLLNSILGVKPTSVPFNGTGPALTALIAGQVD
                     YMCDQIVNIVQQVQAGQIKAYAVGTPERSPALPNVPTSQQAGLPEFQAQAWNALFAPK
                     DTPQPVLDKLTDALDKALDDEQTRKRLLELGSVLPGKGERGQQALAVLVKREIERWTP
                     IIKAAGPTD"
BASE COUNT          314 a          545 c          628 g          331 t
ORIGIN      
        1 acgccattgt cggcgaccgc ggcgacgagg acgatgtcga tgcgcgatcc cgcgggcggc
       61 tcgctcacac cgcgaccttg gccttgatgt gcgggtacgg gtcgtagccg tggagcgaga
      121 aatcttcgta gtggaaggca aagagatcgc gcactgcggg attgagcgtc atccgcggca
      181 gcgatcgcgg aacgcgcgag agctgcagct gcgcctgttc caggtgattg cgataaagat
      241 gcgcgtctcc cagggtgtgc acgaactcgc cgggtttgag tccggtcacc tgtgccacca
      301 tcagcgtcaa caacgcgtaa gaggcgatgt tgaacggcac gccgaggaag acgtcggccg
      361 agcgctgata gagctggcac gacagccgac cggtggcgac gtaaaactgg aataggcagt
      421 ggcatggcgg cagcgccatc ttgtcgacat cggccggatt ccacgccgtc acgatcaggc
      481 ggcgcgagtc gggattgcgg cggatggcgg caaccacgtc ggcgatctgg tcgatcacgc
      541 cgccgtcggg agtaggccac gagcgccatt gccgcccata gaccggaccc agttcgccgc
      601 tctcgtccgc ccagtcgtcc cagatcgtca cgccatgttc gttgagatat ttgacgttcg
      661 tgtcgccggc gaggaaccag agtaactcgt gcacgatcga tctgaggtgc agttttttgg
      721 tggtggtgag gggaaagccc tgcgcgagat cgaaccgcat ctgatgaccg aacaccgaca
      781 gcgtgccggt cccggtgcgg tcgtgcttct ccgtgccgtc ggcgaggatg cgttcgatca
      841 agtcgagata ttggcgcatg gctgcggtcc tcaggcggcg aaagacccga gtcggacggc
      901 cgagtgtacc gccctcccgc ttgccggcaa acgtgcagaa cgacttgtga atataagcgt
      961 cagtcggtcg ggccggccgc cttgatgatc ggcgtccagc gctcgatctc gcgcttgacc
     1021 aacaccgcga gcgcctgctg gccgcgctcg cccttgcccg gcagcacgct cccgagctcc
     1081 agcaggcgtt tgcgggtctg ctcgtcgtcg agcgccttgt ccagcgcgtc ggtgagcttg
     1141 tcgaggacgg gctggggcgt gtctttcggg gcgaataggg cgttccaggc ttgcgcctga
     1201 aactccggca gccctgcctg ctggctggtc ggcacgttgg gcagcgccgg actgcgctcg
     1261 ggcgtgccga ccgcataggc cttgatctga ccggcctgca cctgctggac gatgttgacg
     1321 atctggtcgc acatgtagtc gacctgtccg gcgatcaagg ccgtcagcgc cgggccggtt
     1381 ccgttgaagg ggacggacgt cggcttcacg cccaggatcg agttgagcaa caagcacgtc
     1441 gtgaacgaca ccgatccgac cccggcgtgg gccatgttga ggctggcgac attggccttt
     1501 acgtaggtaa cgaactccct gagatcgttg ggaggaaagt ccttcttcgc cagaatcagg
     1561 accggcgtgc ccgcgaccat gccgatgggc gcaaaatcca catcaggttt gtaggcaaga
     1621 ttcgggtaga gcgcgaccga ggcggcgtgg gttcccatat gacccatctc gatcgtgtag
     1681 ccgtcggggg tggcgcgtgc cgcccgcgtc gaggcggtcg tgccgccggc accgagcgtg
     1741 ttctcgatga cgatggactg gccgagggtg cgagccatgt gatcgctgat gatgcgggaa
     1801 atcacgtcgg tcggtccg
//
LOCUS       DATAQL010001160         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1338578, whole genome shotgun sequence.
ACCESSION   DATAQL010001160 DATAQL010000000
VERSION     DATAQL010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338578"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VHS97_20045"
     CDS             <1..1446
                     /locus_tag="VHS97_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3450557.1"
                     /translation="RADGASAIRVLDVNGQSTVIPRSQIEELRPSTTSIMPAGLAGAL
                     GEARMRDLLAFLKASSAIRPGSGSPPSADALAVSANGAPGLEPKASTSPALPRLATQV
                     AFPNLQFERPVAMAYPDEGGNLLFVVEQRGRIWSFPNVPDTTTKAEFLDIRPKVYSPA
                     SGGHNEEGLLGLAFHPGYRNNGEFFVYYSAHEGPTGRRSVVARYKVSRDDHHRADPAS
                     EQRIWVGPPDPYGNHNGGTIAFGPDGFLYITLGDSGAADDPLTTGQDPSDWFGSILRI
                     DVDHPGEGKPYGIPSDNPRLRDPKRFGRWAPEVYCIGLRNVWKFSFDRMTGALWAGEV
                     GQNLWEMVHVIENGGNYGWSIKEGFHSFRPRQPKDPASPLSPPLAEYPHAPNQGGSGR
                     ADDGKSITGGHVYRGRRLPDLAGTYVYADYDTGRIWGLRAQDGKVLANGELIDRTRQS
                     HLNVASFGEDSQGELYILAFDGRIYWLVARE"
     gene            1539..2567
                     /locus_tag="VHS97_20050"
     CDS             1539..2567
                     /locus_tag="VHS97_20050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3450558.1"
                     /translation="MTPQDPNRREFLQAGVAASVALGLTEPASAQATPKNDGGIPLRK
                     FGRTDRMVTIMALGGHACTNPKKLSESESLRLIRRAVDEGITFMDNCWDYHDGEAEAR
                     MGKALAEGGRREKVFLMTKVCGRTANDARSHLEDSLRRLKTDHLDLWMFHEIIYDNDP
                     DWIFERQGAIETGLRALEEGKVRYLGFTGHKDPSIHLKMLSKPYDWTAVLMPLNVMDV
                     HYRSFQKNVLPELNRRNIAPLGMKSLGGNGSIVTAAGHSPQDAIRYVLSLPICSLVSG
                     IDSEKVLDQNLEILRHFMPVTEKERQAIEQRSARLAGDGRFELFKSSKLFDGPVHRKQ
                     HGFDTELS"
BASE COUNT          526 a          843 c          806 g          449 t
ORIGIN      
        1 cgcgccgacg gggccagtgc gatccgtgtg cttgatgtca acggccagtc gacggtgata
       61 ccgcggtctc aaattgaaga gcttcgcccc agcacgacat cgatcatgcc ggccggactg
      121 gccggtgcgc tgggcgaggc ccgaatgcgc gacctcttgg cctttctgaa ggcgtcgtcg
      181 gccatccggc ccggctcggg atcacccccc tcagccgatg ctcttgcggt atcggcgaat
      241 ggcgcgcccg gattggaacc gaaagcgtcg acaagtccgg cgctgccgcg ccttgccacc
      301 caggtcgctt ttccaaacct ccagttcgag cgaccggtcg ccatggcgta tcccgacgag
      361 ggcggcaact tgcttttcgt cgtcgagcag cgcggccgta tctggtcgtt cccgaacgtg
      421 ccggacacta ccacaaaggc agagttcctg gacatccgcc cgaaggtcta cagccccgct
      481 agcggcggtc acaacgaaga gggactgctc ggcctggcgt ttcatcccgg ctaccggaac
      541 aacggcgagt tcttcgtgta ttactcggcg catgaaggac cgacgggacg ccggtctgtc
      601 gtcgcgcgtt acaaggtctc ccgcgacgat caccaccgtg ccgacccggc cagcgagcag
      661 cgaatctggg tcgggcctcc cgatccgtac ggcaaccaca acggcggcac gatcgccttc
      721 ggccccgacg gctttctcta catcacgctg ggcgacagcg gcgcggcaga cgaccccttg
      781 acgaccggcc aggaccccag cgactggttc ggttcgatct tacggatcga cgttgatcac
      841 ccaggcgaag gcaaaccgta cggaattccg agcgacaatc cacggcttcg tgatcccaag
      901 cggtttggcc gctgggcgcc cgaggtctat tgcatcggtc tgcggaacgt atggaagttt
      961 agcttcgacc ggatgaccgg cgcgctttgg gcgggtgaag ttggccaaaa cctgtgggag
     1021 atggtgcacg ttatcgagaa cggcggcaac tacggctgga gcataaagga gggatttcat
     1081 agtttccgcc cgcgccagcc gaaggacccc gcctcgccgc tctcgccccc tttggccgag
     1141 tatccacacg ctccgaatca aggcggctcc ggccgcgccg acgacggcaa gagcatcacc
     1201 ggcggccatg tctaccgggg ccgaagattg cccgatctgg ccggcacgta cgtctatgcc
     1261 gactacgaca ccggacggat ctgggggctc cgcgcgcagg acggcaaggt actcgcaaat
     1321 ggcgagttga tcgatcgaac ccgacagtcg cacctgaatg tcgcctcctt cggagaggat
     1381 tcccaggggg agctatacat cctggccttc gacggccgga tctactggct ggtcgcccga
     1441 gaatgaactg ggtaagaaga gaagtagaac cagcggaacg gtcccctccg ggcgcgccgc
     1501 ccggatcgag ggaccgatcc catggaggct atgaaaccat gacgccacaa gacccgaatc
     1561 gccgcgagtt tctccaagca ggcgttgccg cgagcgtcgc gctcggcctc accgagccgg
     1621 cctcagccca agcaacgccg aagaacgacg ggggcatccc cctgcgcaag ttcggccgca
     1681 ccgaccggat ggtcaccatc atggccctgg gcggtcatgc gtgcaccaac cccaaaaagc
     1741 tttcggagtc ggagagcctt cgcctcatcc gacgcgctgt cgacgaaggc atcaccttca
     1801 tggacaactg ctgggactac cacgacggcg aggcggaggc gcggatgggc aaggccctcg
     1861 ccgaaggcgg ccgccgggag aaggtcttcc tcatgaccaa ggtctgcggc cggaccgcca
     1921 atgacgcgcg ctctcacttg gaggacagtc tccggcgact caagacggat catcttgacc
     1981 tgtggatgtt tcacgagatc atctacgaca atgacccgga ctggatcttc gaacgccagg
     2041 gagcaatcga gaccggcctg cgggcactgg aagagggaaa ggttcgctac ctcggtttca
     2101 ccggccacaa agacccatcg atccacctga agatgctgtc caagccctac gattggacgg
     2161 ccgtgctcat gcccttgaac gtgatggacg ttcactaccg cagttttcaa aagaatgtct
     2221 tgcccgagct gaaccggcga aacatcgcgc cgttgggtat gaagagtcta ggcggcaacg
     2281 gctcgatcgt caccgcggcg ggacattcgc ctcaggacgc gatccgctac gtcctatcgc
     2341 tgccaatctg tagtctggtc tcgggcatcg acagcgagaa ggtactcgac cagaacctcg
     2401 agatcctccg ccactttatg ccagtaaccg agaaggagcg ccaggccatc gaacagaggt
     2461 ccgcccgtct ggcgggagac ggccggtttg agctattcaa gtcatcgaaa ttgttcgacg
     2521 gcccggtgca ccgcaaacag cacggattcg acaccgagct gtcttgacga gtcagacgtg
     2581 gctcccttag gaggatgatg tccggaatcg tgcctttgtc ggat
//
LOCUS       DATAQL010001161         4573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1187223, whole genome shotgun sequence.
ACCESSION   DATAQL010001161 DATAQL010000000
VERSION     DATAQL010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187223"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /locus_tag="VHS97_20055"
     CDS             <1..2632
                     /locus_tag="VHS97_20055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3450559.1"
                     /translation="LFPDPTRPKVTVNLTVLVGSRHEGYGETGMAHLLEHMVFKGTPT
                     HRDIPSAMKERGAQFNGSTDVDRTNYFETLPATDQNLEFAIRLEADRMVNSPIKAEDL
                     ATEFSVVRNEFESGENSPYRVLSQRMAAVAYEWHNYGKSTIGNRSDIERVPVDNLRAF
                     YTKYYQPDNAMLVVAGKFDEKKALEYITKYFGALPRPDRKIPVTYTEEPAQDGERSVT
                     LRRVGDVGLVGLLYHVPAASHGEYPAVEVLGNILDSEPSGRLYKALVESKKAARVSTR
                     PSATHDPGMIEIVAEVNTKDPATLENVRDAMLAVLDQVASSGVSQEEVDRARQKILKD
                     RELAAADPNRIAVELSEWGAQGDWRLYFLNRDRIEQVTPAQVKDIASKYFATSNRTVG
                     FFVPTAKAERTMIPAVPDIAKLLNGYEGRATKSESSETNDVAPLAIEARVVRPEPIGG
                     VKLAFLPRKSRGESVQLRLALHYGSAENLKGLSDASSLLPELMIRETKSLNRQQIQDA
                     LDKNFARLGTGGPMGRGRGMRGGGGGGPGTLTFTVQTRRANFAAVLEILRQILREATL
                     PATEFDLIKNERIAALEQGRSDPMALGMIQIQRLLSPYPSDDVRYVPTVVERVDRLKK
                     VSLDKVKSLYQSYLGADHGELVIVGDFEPSEIMPILARTFDGWKAEKPYARIERPYQA
                     DLKSERITIQTPDKDNALYVAGLCLPIMDDNSEYPALAIGNFILGGGGLSSRIANRLR
                     QKDGLSYGAGSFFGASPVDPRADLLLNAIYNPINVAKVVSDVDEELERLLKDGVTAAE
                     LDTAKTGYLQQEQNMRTNDMAITGTLSENLFVGRTMQFQAELEQKIKDLTPEAVNAAL
                     RKHIDPHRLSVVTAGDFNKK"
     gene            2643..3722
                     /locus_tag="VHS97_20060"
     CDS             2643..3722
                     /locus_tag="VHS97_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX3450560.1"
                     /translation="MLRRDFLKKTGAAALASAAANGGRGPFVHASDKTGSSNPVIGKG
                     EYRYECFHDWAHVPDSVRWYETHGVAIDKAGFVYIKHRVASQKPKSREDARDTIVVFD
                     PQGNFVRSFGKEYHGGGHGIDIREENGQEFLYLSCMMPVNLVVKTDLKGEVVWTKVAP
                     SESHAYDKPGTPFAPTNVAFAPDGGFYVGDGYGSNYIHQYDRDARWVRTFGGTGTDSG
                     KFRTPHGLWLDNRPGRETQLVVADRANARLQYLTLDGKPISIDHDVSFPAHFDIRGEV
                     LLVPDLHARVSLFDKNNDVIVHLGYDPEWTKQVLDGFKIRTQPDRWPAGKFIHPHDAC
                     FDKDGNIFVAEWVQTGRVSFLRHVS"
     gene            3767..4324
                     /locus_tag="VHS97_20065"
     CDS             3767..4324
                     /locus_tag="VHS97_20065"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX3450561.1"
                     /translation="MDMLQGHLLISSPELADPNFAKSVVLIAVHGEEGALGLILNREM
                     NTPMSQVWEKVSQSECLRTDNVRHGGPVSGSLMALHDQRPLANLVVTDLIYVATELNA
                     MEALASSDDGQAFFFIGHSGWGPGQLETEMADGSWLVLPATAGHVFGSRDLSVLWKDC
                     VIEVGRRQVQAVVPTKHVPDNPRLN"
BASE COUNT          959 a         1414 c         1353 g          847 t
ORIGIN      
        1 cttgttcccc gatccgacgc gccccaaagt cacggtgaac ctcacggtat tggtcggttc
       61 gcggcacgag ggctacggcg aaacgggcat ggctcacctt ctggagcaca tggtcttcaa
      121 gggaacaccg acccaccgcg atatccccag cgcgatgaaa gaacgcgggg ctcagttcaa
      181 cggctctacc gatgtcgacc ggaccaatta cttcgagacg ctccccgcca cggaccagaa
      241 cctcgagttc gccattcgcc tcgaggccga ccgcatggtc aacagcccca tcaaggccga
      301 ggatcttgca accgagtttt cggtggtgcg caacgagttt gaatcgggcg aaaactcccc
      361 ttatcgtgtg ctgtcgcagc gaatggcggc agtcgcctac gagtggcaca actacggcaa
      421 gtcaacgatc ggcaaccgct ctgacatcga gcgggtaccg gtcgacaatt tgcgggcctt
      481 ctacaccaaa tactaccagc ccgacaatgc catgctggtc gtcgccggca agttcgatga
      541 aaagaaagca cttgaatata tcacaaaata ttttggcgct ttgccgcgac ccgatcgcaa
      601 gatccccgtc acctacacgg aagagccggc gcaagacggc gagcggtcgg tcaccttacg
      661 ccgcgtggga gacgtggggt tagttggact gctctatcat gttcccgccg cctcgcatgg
      721 cgagtacccc gcggtggaag tcttgggcaa tatcctcgat tccgagccgt caggccgact
      781 ctacaaggcc ctggtcgagt cgaagaaggc agcgcgggtc tccaccaggc cgtcggcgac
      841 tcacgacccg ggcatgatcg agatcgtcgc cgaggtcaac accaaggatc cggcgaccct
      901 cgagaatgtc cgggatgcca tgctcgcggt cttggatcag gtggcctctt cgggtgtgag
      961 ccaggaggaa gtcgatcgcg cccgccagaa gatcctcaag gatcgtgagc tggcggccgc
     1021 cgatcctaac cgcatcgccg tcgaactctc ggaatggggc gcacaaggag actggcggct
     1081 ctatttcctc aatcgcgacc ggatcgagca ggtcacgccg gctcaggtga aggacatcgc
     1141 ctctaaatac ttcgccacca gtaaccggac cgtcggcttt ttcgttccga cggccaaggc
     1201 cgagcggacg atgattccgg ccgtgcccga tatcgccaag cttttgaacg gttacgaggg
     1261 acgcgccacc aagtcggaat cgagtgagac caacgacgtc gccccactgg ccatcgaagc
     1321 acgggtcgtg cgccccgagc caatcggagg agtcaagctg gctttccttc cccgaaaaag
     1381 ccgaggcgag tcggtacagc tccgcctggc gctccactac ggcagcgcgg aaaacctcaa
     1441 gggtttgagt gacgcctcca gccttctgcc cgaattgatg atccgggaaa ccaagagctt
     1501 gaatcgccag cagattcagg atgctctcga caaaaacttc gcgcggctcg gtaccggcgg
     1561 ccccatgggt aggggccggg gtatgagagg aggcggcggc ggcggcccgg gcaccctgac
     1621 cttcacggtc cagaccaggc gcgctaactt cgccgccgtc ctcgagatcc tgcgccagat
     1681 cctccgtgag gccaccctgc ccgccactga attcgacctg atcaaaaacg agcggatcgc
     1741 cgccttggag cagggccgat ctgatcccat ggccctgggg atgatccaga tccaacggtt
     1801 actgtcaccg tatcccagcg acgacgtccg ctatgtgccg acggtcgtcg aacgcgtgga
     1861 tcgcctcaaa aaagtctcgc tcgacaaggt taagtcgctc tatcaatcct atcttggggc
     1921 tgatcacggc gagcttgtga tcgtcggcga cttcgagcct tccgagatca tgccgattct
     1981 ggccaggacc ttcgacggct ggaaggctga aaaaccctac gctcggatcg aacgcccgta
     2041 ccaggctgac ctgaagtccg agcgcatcac catccagact cccgacaagg ataacgccct
     2101 gtacgtggcg ggcctctgcc tgccgatcat ggacgacaat tccgagtatc cggcgcttgc
     2161 tatcggcaac ttcatcctcg gaggaggcgg cctttcgtcg cgcatcgcca accggctccg
     2221 ccagaaggac ggactatctt acggggcggg atcgttcttt ggtgccagtc cggttgatcc
     2281 cagagccgac ctgctcttga acgcaatcta taatccgatc aacgttgcca aggttgtctc
     2341 ggacgttgac gaagagctag aacgcctgct caaagacggt gtcacggccg ccgagctcga
     2401 cacggccaag accggatacc tgcagcagga gcaaaacatg cgtacgaacg acatggcgat
     2461 cacagggaca ctgtcggaaa acctgttcgt ggggcggacc atgcagttcc aggccgagct
     2521 cgagcaaaag atcaaggact tgacaccgga agccgtcaac gccgccttgc gcaaacacat
     2581 agaccctcat cggctttctg ttgttactgc gggcgatttt aacaagaagt aggacaaata
     2641 cgatgctgag acgcgacttc ctgaaaaaga ccggcgcggc ggctctggcc tcggctgccg
     2701 ccaatggcgg ccgtgggccg tttgtgcatg ccagcgacaa gacagggagc agtaaccctg
     2761 tcatcggcaa aggtgaatat cgatacgaat gttttcatga ttgggcacac gttcccgatt
     2821 ccgtgcgctg gtatgagacg cacggggtgg cgattgacaa ggcgggcttc gtttacatca
     2881 aacaccgagt cgccagccag aagccgaaat cacgtgaaga cgcgcgtgac acgatcgtcg
     2941 tattcgaccc ccagggcaac ttcgtgcgat ccttcggtaa ggaataccat ggcggcggac
     3001 acggtatcga catccgtgag gaaaacgggc aggagtttct ctatctttcg tgcatgatgc
     3061 cggtaaatct ggtggtcaag acggacttga agggggaagt cgtctggacc aaggttgcgc
     3121 cgagcgagtc gcacgcctac gacaagccag ggacgccatt cgcccccacc aatgtggcgt
     3181 ttgctcccga cgggggattt tacgtcggcg acggttacgg ctcgaactac attcaccagt
     3241 acgacagaga cgccaggtgg gtacggacgt ttggcggtac aggcacagat tccggcaagt
     3301 tccggacgcc gcacggactc tggctcgaca acaggcccgg ccgcgagacc cagctcgtcg
     3361 tggctgaccg tgccaacgca cgactccagt acctgacgct cgacggcaaa ccgatcagca
     3421 tcgaccacga tgtcagcttc ccggcccact tcgacattcg cggtgaggtg ttactggtac
     3481 cagacctgca cgcccgcgtc agtttgttcg acaagaacaa tgatgtcatc gtccacttgg
     3541 gctatgaccc tgaatggacc aagcaggttc ttgacggctt caagatccgc actcagcccg
     3601 accgctggcc ggccgggaag ttcattcatc cccatgacgc ctgctttgac aaagatggta
     3661 atatcttcgt cgccgaatgg gtgcagaccg gccgggtttc gtttttgcgt cacgtgagct
     3721 gactcccggt tgctcatagc ctcgtgccgg ctgccgctct atcatcatgg atatgcttca
     3781 gggacacctg ctgatttcat ctccggaatt ggccgacccg aacttcgcca agtcggtcgt
     3841 cttgatcgcc gtgcatggcg aggaaggagc gctcgggttg atcttgaacc gtgagatgaa
     3901 tacgccgatg tcgcaggtct gggagaaggt cagccagtcg gagtgcctgc gtaccgacaa
     3961 cgtgcggcat ggcgggcccg tcagcggcag cttgatggcc ctgcacgacc agcggcccct
     4021 cgccaacctc gtcgtgaccg accttatcta cgtcgccacc gagctcaatg ccatggaggc
     4081 attggcgtcg tcggacgatg gacaggcttt cttcttcatc ggccattccg gctggggacc
     4141 agggcagctc gaaaccgaga tggccgatgg atcgtggctg gttctcccag ccacagccgg
     4201 ccatgtcttt gggagccgcg acttgagcgt gctctggaaa gattgcgtca tcgaggtcgg
     4261 ccgccgccaa gtccaggccg tggtcccgac caaacatgtg cccgataacc cgaggctcaa
     4321 ttgagactgg gtttgccccg attccgatga attagcagcg aattgccggt gcacagcaat
     4381 gtacgatccc ctcccccctt gtgggggagg gccaggttgg ggggcgtccc cgcagggacc
     4441 tcagccgacc aaaaattgcg gcgcctgccg ccccccaccc caaccctccc ccacaggggg
     4501 ggagggagtc agcagggagg cggctttgga cttgacttcc gccgaccgtg acgggcaccg
     4561 tcccgcccgc ttg
//
LOCUS       DATAQL010001162         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1949761, whole genome shotgun sequence.
ACCESSION   DATAQL010001162 DATAQL010000000
VERSION     DATAQL010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949761"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VHS97_20070"
     CDS             complement(<1..527)
                     /locus_tag="VHS97_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450562.1"
                     /translation="MRFFPRLIFIAATGCIAAAGGVIYWRARAHHVVKSNPAPHVPQS
                     TPRVSLSAPYPGPGLTTLPDPAAPPGSSSQVFIDRQSFDGLVAAVTAQFTDKVRDQSS
                     LEEYRQAIAHRAERAKAQLRERLSLSHLDTTPTLEQASHAISIYSQLAFVGLYEGDHD
                     ETAVWLKKAMALSRTP"
     gene            complement(665..2056)
                     /locus_tag="VHS97_20075"
     CDS             complement(665..2056)
                     /locus_tag="VHS97_20075"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX3450563.1"
                     /translation="MRRTICFFVVASVLNTIPSMCAAEQVAVGAAVVDVTPDYPIRMV
                     GYESRKTESEGIASRLKARALAIGADGEANGGPAVLVAVDNCAVGAKVPEEVAARLNR
                     KAKLPRDRFVVCSTHTHCAPALSSGLDFIFSGPLPADQRSRIERYTRELTDAIEKAAL
                     EALTARTPASLAWGQGRVGFAANRRVLKDGRWVGFGVNPNGPVDHNVPVLRATDAAGN
                     VKAVLFGYACHCTTLGGEFNKICGEWTGYACDEIEKEAPGAIALAIIGCGADANPEPR
                     RNLDDAKKHGAAAGREVNRLVTSTLTPLPGRIQSRLRRIQLPLEPLPTPAKLEDQTKV
                     SGAAGFLARTLIERLNRGESLPTTVPYVVQTWCFGDEMAIVFLAGEVVVDYALRLKWE
                     IDADRLWVAAYSNDVPCYIPSRRVLSEGGYEADFSMIYYGHASRFAPATEDLIIQTVH
                     ALLPAPFDGPRKP"
BASE COUNT          431 a          751 c          707 g          431 t
ORIGIN      
        1 ggcgtcctcg aaagcgccat cgccttcttg agccagaccg ccgtttcgtc gtggtcacct
       61 tcgtacaggc cgacaaacgc caattgtgag taaatcgaga ttgcatgtga cgcctgctcc
      121 aaagtgggcg tggtgtcgag atgcgaaagc gatagcctct ctcgaagctg tgccttcgcg
      181 cgttcagcgc ggtgggcgat cgcctggcgg tactcctcga gcgagctttg gtcgcggacc
      241 ttgtcggtga actgcgctgt caccgccgcc actaaaccgt cgaacgattg tcgatcgata
      301 aagacctgcg acgaagaacc gggcggcgct gccgggtctg gcaacgtcgt caggccgggg
      361 cctggatacg gcgctgacag ggacacgcgg ggggtggact gtgggacatg aggggcggga
      421 ttcgatttga cgacatgatg cgctcgcgcg cgccagtaaa ttacaccgcc tgcagcggcg
      481 atgcagcccg tcgccgcgat gaagatcaat cgcgggaaga atcgcacctt gggcctccct
      541 tcaggcccgg cccgtgagcc ctcgaagcac gaaatccgca cgttatcttt attatcacac
      601 ctggcggcat gtctcgctat tgatatctcc ccggtccgta accctttgag tggaagctca
      661 gtctctacgg cttccgcggc ccgtcgaacg gagcaggcaa cagagcgtga acggtctgga
      721 tgatgaggtc ttctgtcgcc ggcgcgaacc gcgaggcgtg gccgtagtag atcatcgaga
      781 agtccgcctc gtaacctcct tcgctcaaga cccgacgcga cgggatgtag caagggacgt
      841 cgttgctgta ggcggcgacc cagagccggt cggcgtcgat ctcccacttg agccgcaagg
      901 catagtcgac gacgacctct cccgcgagga agactatcgc catctcgtcg ccgaagcacc
      961 aggtctggac gacgtacggc actgtcgtgg ggagagattc tcctcggttg agccgctcga
     1021 tcaaggtccg cgcgaggaac ccggccgctc ccgacacctt ggtctgatcc tccagcttgg
     1081 ccggcgtggg aagcggttcc aacggcagtt ggatcctccg caaacggctc tggattcggc
     1141 cgggcagggg agtgagagtc gacgttacca atcggttgac ctcgcgaccg gcggccgcac
     1201 cgtgtttctt cgcgtcgtcg aggttgcgcc tcggctcggg attggcgtcc gccccacaac
     1261 cgatgatagc cagcgcgatc gctccgggtg cttccttttc aatctcgtcg cacgcgtatc
     1321 cggtccattc gccgcagatc ttgttaaact cgccccccag ggtcgtgcag tggcaggcgt
     1381 agccgaagag gacagccttg acgttgccgg ccgcgtcggt ggctcgtagc accggcacgt
     1441 tatgatcgac aggtccgtta ggattcaccc caaagccgac ccagcggccg tccttgagca
     1501 ccctgcggtt ggccgcgaag ccgacacgac cctgacccca cgccaggctc gccggagttc
     1561 gggccgtcag ggcctcgagc gcggcctttt cgatcgcgtc ggtcagctcg cgcgtgtagc
     1621 gctcgatccg cgatctctga tcggccggca acggaccgga aaagatgaag tcaagcccgc
     1681 tgctcaaggc cggagcgcag tgcgtgtgtg tcgagcagac gacgaaccgg tcacgcggca
     1741 gcttcgcctt tcggttcagc cgcgccgcca cttcctcggg taccttcgcc ccgaccgcgc
     1801 aattgtcgac ggccaccagt acagccggcc cgccgttcgc ctcgccatcg gccccaatcg
     1861 ccagagcacg cgccttgagc cggctggcga ttccctccga ctcggtcttg cgcgactcgt
     1921 agccgaccat ccggatcgga taatccggcg tgacgtccac gacggccgcg ccaaccgcca
     1981 cttgttcggc cgcacacatt gagggaatcg tattcaagac agacgccacg acaaagaagc
     2041 agattgtacg tcgcattacc gttgctctcc catacggcca gacgcccact actgaccaat
     2101 gaccaatgac caatgaccaa tgaccaatga ccaatgacca atgaccaatg actaatatcg
     2161 ccagctcgtc acatcaccgc cagacggagc agtcttcaag agtcgctcga cgattttggg
     2221 tgcgtacatc tggtggtagc gcagccggct gatggcgttg ggtcgtgtgg ggtcgccgtt
     2281 ccagcagtgc tcggcacggt cgccatataa cacctcgccg
//
LOCUS       DATAQL010001163         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2865944, whole genome shotgun sequence.
ACCESSION   DATAQL010001163 DATAQL010000000
VERSION     DATAQL010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865944"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VHS97_20080"
     CDS             <1..690
                     /locus_tag="VHS97_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX3450564.1"
                     /translation="LYRLPDSGFDYAAVGVRPDWSHHQTGFAAHWDKNSNLAWAFDTW
                     FLNLFPRARPFTHNGGGYATLSFIPTLATMVLGLMGGEVLRRPGRGWAKVSWLFAAGA
                     VGLATGWGLGELGICPVVKRIWTPSWVLYSGGWCFLFLAGFYAILDLIGVRFWAFPLR
                     VIGMNSIAAYCMAGLWESFIARNLTIHLGKNAFTVIGQPYEPLIHGAAVLFVLWLILF
                     WMYRRRLFLRI"
     gene            770..1204
                     /locus_tag="VHS97_20085"
     CDS             770..1204
                     /locus_tag="VHS97_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450565.1"
                     /translation="METPTMGKVLVTAKIENLEDLYSAKRGLLPDDQVRRIEVHDAVV
                     DTCATTLLLPKRMIAALGLEPLRTRHSRGLGGDFLLPVFGTVRLTIQGRDCALDVGEI
                     GDEYPVLVGQIPLESLDWVVDAKGQRLIGNPDHGGEWGMDAF"
     gene            1407..>3841
                     /locus_tag="VHS97_20090"
     CDS             1407..>3841
                     /locus_tag="VHS97_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX3450566.1"
                     /translation="MVVGIGCSKTQGADQNSSPLRLEVTRDTWVSEVGPEADGNNGSA
                     PRLKLKSIQEMSLVDIDTSLLAGRTVRSAVLHLKKTGDETLKRVTVSSVGAEWVEGTG
                     SGYTIQPGSATFRRRRHPDLPWSIAGGDICHVVLGNGGTLWRMADASPPDSDGWQHIP
                     VDPRVVAARVAGLSYGFLLFDDTGSEWTRTGDTFTQRIFPNRFVYSREQNRSSAPYFT
                     IELGPEDRRPPPAPSDLRVDPGTSLLPPGEALVSWVTPPDDDAQRTLGFFVILNDRPV
                     PRELIPLAVEAGGRVVMHLRDLKLEPERTHKLSVQAVDGAGNRGTAASAEITVSRFMP
                     TPLPQPKPPPAAVPSPTVTAWPQIAGMQVAIIDELDKVHPATGELIPPEFRDYLATNH
                     LWNAETRTIALHAARNEFVAFQVLIRGNNTAALITPALIFDGQVGKTIPVELGRYFHV
                     PTTLGALPDPVVPLNFPTRPTPAAKSQGLHVEVYVPHSTLAGLHRGFLTLSSPTTNEA
                     LRLPVELTVWDFTLPDHLSFVPEMNSYDLPENERDYYRLAHRHRTVLNRLPYHQNGNV
                     SPGCAPLWDSRRLVFGWSQWDRRFGPLLDGSAFADLPRKNVPVECFYLPLHESWPSPM
                     EGNYNGDYWADRAFPESYRRAFVAAARQMAAHFQTKQWNQTLFHGFLNNKNNFKTRGW
                     STASSPWLLDEPANFQDYWAIQYFARAFHEGINQATGGTTPATASLPRMVFRADISRP
                     QWQRDTLDGLLDYNVVSSAVRTYPLLVMDRKRTWGQIVVEYGSTNPVEGSNLQPVAWC
                     LDAWSMGLDGVLP"
BASE COUNT          707 a         1238 c         1119 g          777 t
ORIGIN      
        1 ctttatcggc tccccgactc gggttttgat tatgccgccg tcggcgtgcg gccggattgg
       61 tctcatcatc agaccggatt cgcggcgcac tgggataaaa acagcaatct agcctgggcg
      121 tttgacacgt ggttcttgaa cctgttcccg cgcgcgcggc cgttcacaca taacggcggc
      181 ggctacgcga ccctgagctt catccccacg ctggcgacga tggtcctcgg tctaatgggc
      241 ggcgaagtat tgcggcggcc tgggcgcggc tgggccaagg tgtcgtggct cttcgcggct
      301 ggtgccgtcg gcctggcgac cggttggggt ctgggtgagc taggcatctg ccccgtcgtc
      361 aagcggatct ggaccccgag ctgggtgctc tatagcggcg gctggtgctt cctgtttctg
      421 gcgggatttt acgccattct cgatcttatc ggggtgcgtt tctgggcctt tccgctccgt
      481 gtgatcggta tgaactcgat cgcggcctat tgcatggccg gcctctggga aagctttatc
      541 gcgcgaaatc tgaccatcca cctggggaag aacgccttca ccgtgatcgg ccagccgtac
      601 gagccgctca tccatggggc ggccgtgctc ttcgtgctct ggctcatcct gttctggatg
      661 tatcggcgca ggcttttctt gagaatatga taagagtctg gcgaacgcga ccttacccta
      721 ccgggcattg gctcaattcc acacatttct gtaaagggag tagagcatca tggagacacc
      781 gacgatgggc aaggtcctcg tgaccgccaa gatcgaaaac ctggaagacc tgtacagtgc
      841 aaagagagga cttttacccg acgaccaagt ccgtcggatc gaagttcatg atgccgttgt
      901 cgacacctgc gcgaccacgc tcttgcttcc gaaacgcatg attgccgcgc tgggactcga
      961 accactccgt acgcggcact cgcgcggatt gggcggcgac tttctactgc ccgtctttgg
     1021 caccgtgcgg ctcaccatcc agggccgcga ctgtgctctt gatgtcggtg agatcggcga
     1081 tgaataccct gtcttggtgg gccagattcc tctggaatcg ctcgactggg tcgtcgacgc
     1141 caagggacag cggctgatcg gcaatcccga ccacggcggc gagtggggaa tggacgcgtt
     1201 ttgagcgatc gcagttaaat ctactgcacg caggccgctt gaggcacccc gggagggcga
     1261 gccgactccc aacgccagct cggccggagc ctcgccctcc caacatgcaa cgattccgcc
     1321 cgcacggtgt ataagtttcc tatacaacaa gccatggctc cgtctctatg cttcatagca
     1381 gagtttctaa atttctgacg attttcatgg tcgtcgggat aggttgctcc aaaacgcaag
     1441 gtgcagacca aaatagctcc cctttgcgcc tcgaggtgac gcgcgacaca tgggtctctg
     1501 aagtcggccc agaagccgat ggcaacaatg gcagcgcacc ccggctcaag ctcaagagca
     1561 tccaggaaat gagcttggtc gatatcgata cgagcctcct tgccggacgt acggtccgat
     1621 cggccgtatt gcacttgaaa aagaccggcg atgaaacgct caagcgggtg acggtcagca
     1681 gtgtcggagc ggagtgggtc gaggggaccg gcagcggcta cacgatccag cccggcagtg
     1741 cgaccttccg tcgccgccgg caccctgatc tgccgtggtc gattgccgga ggcgatatct
     1801 gccatgtcgt ccttggcaat gggggcacac tctggcgaat ggccgacgca tcgccgccgg
     1861 attccgatgg ctggcagcac atcccggtcg acccgcgcgt cgtggcggcc cgcgtggctg
     1921 gcctctccta tggcttcctg ctctttgatg acaccggctc ggaatggacg agaaccggcg
     1981 acacgtttac tcagcggatt ttccccaatc gttttgttta tagtcgcgag caaaaccggt
     2041 cgagcgctcc ttatttcact atcgagctcg ggccggaaga ccgccggccg ccgcctgccc
     2101 cgagcgatct acgggtcgat ccggggacgt ctttattacc gcccggtgaa gctcttgtct
     2161 catgggttac tccgcctgac gacgacgccc aacgaacgct cggctttttc gtgatcttga
     2221 atgatcggcc cgtaccgcgc gagctgatcc cgctcgcagt cgaagccggc gggcgggtcg
     2281 tgatgcacct ccgcgacctg aagctcgaac ccgaacgaac gcacaagctc tcggtgcagg
     2341 ccgtggatgg cgccggcaat cgagggacgg ccgcgtcggc cgagatcacg gtctcgaggt
     2401 tcatgcccac ccccctgccc cagcccaagc cgccaccggc cgccgtgccc agtcccactg
     2461 tcaccgcatg gccgcagatc gccggcatgc aggtcgcaat cattgacgag ctcgataagg
     2521 tccacccggc gaccggtgaa ctgattcccc ccgaattccg cgactacctg gccaccaacc
     2581 atctttggaa cgccgagacc cgcaccatcg cgctccacgc tgcacgcaac gaatttgtcg
     2641 cgttccaggt cttgatccga ggaaacaata ccgccgcgct catcacgccc gcactcatct
     2701 tcgacggaca ggtcggcaag acgatcccgg tcgagctggg ccggtatttc cacgtcccga
     2761 caacgctcgg tgcgctcccg gaccctgtcg ttccgctaaa cttccccacg cgccccacgc
     2821 ccgcagccaa gagccagggt ctgcacgtcg aggtttacgt tcctcatagc acactggccg
     2881 gcctccatcg cgggttcttg accttgagca gcccgacaac caatgaagcg ttacgtctgc
     2941 ccgtggagct cacggtctgg gacttcacac tccccgatca cctcagcttc gtgccggaga
     3001 tgaatagtta cgacctgccc gaaaatgaac gcgactatta ccggttggcc catcggcaca
     3061 ggaccgtttt gaatcggctt ccctaccatc agaacggtaa cgtatcaccg ggctgcgccc
     3121 cgttgtggga cagtcgacgc ctggtcttcg gctggtctca atgggaccgc aggttcggcc
     3181 cgctcttgga tggctctgca ttcgcggatc tgcctcgcaa gaacgtgccc gttgagtgct
     3241 tttacctgcc cctgcacgaa agctggccta gcccgatgga aggcaactac aatggcgatt
     3301 actgggccga ccgtgctttt cccgaatcct atcgccgggc ttttgtcgcg gccgcgcggc
     3361 agatggccgc acacttccag acgaaacaat ggaatcagac cctcttccac ggcttcctca
     3421 acaacaagaa taatttcaag acgcggggct ggtcgaccgc ttcctccccg tggctcctgg
     3481 acgagccggc taatttccag gactactggg ctatccaata cttcgccagg gccttccatg
     3541 aaggaatcaa ccaggccacg ggcggaacca ccccggctac cgcgtcattg cccaggatgg
     3601 tctttcgcgc cgacatctct cggccgcagt ggcagcgcga tactctcgac ggcctgctgg
     3661 actacaacgt cgtcagcagc gcggtccgca cctatccgct gctggtcatg gatcgtaaac
     3721 gaacctgggg ccagatcgtg gtggaatacg gctcgaccaa cccggtcgag ggctcgaacc
     3781 ttcagcccgt cgcctggtgc ctggatgcct ggtcgatggg actcgacggc gttctcccct
     3841 g
//
LOCUS       DATAQL010001164         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3935838, whole genome shotgun sequence.
ACCESSION   DATAQL010001164 DATAQL010000000
VERSION     DATAQL010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3935838"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..2532
                     /gene="shc"
                     /locus_tag="VHS97_20095"
     CDS             502..2532
                     /gene="shc"
                     /locus_tag="VHS97_20095"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX3450567.1"
                     /translation="MIANTDGGVLPNHSERLISRRSAHAALLAHDDLGSALERTKDWL
                     LLRQSEEGFWVGELEGDTILESEYILLLTFLGRETDPVCVKCARYIQDHQLSDGGWAI
                     YPGGPTDISASVKAYFALKLVGISPDDAAMVRARSTILDAGGAHQCNSFTRFYLALLG
                     QISYDECPCVPPELVLIPSRLGFSLSAMSAWTRTIVVPLSIMAYYKPVRTLESERGIA
                     ELFRSDRGQPSRRTARWLSWTNFFLIVDRVLKGLDRSIPALCRRPGIAAAHRWMLEHC
                     ENTDGLGAIFPPMVYSIIALRCLGYEMGSPAVLWAMKQLDDLHIAQGDRVRVQPCLSP
                     VWDTAIAMIALADAQVPADHPSWAPAVEWLLSKEVRNPGDWCIRGPRVEPTGWHFQFH
                     NGFYPDLDDSAMVILALNRSPLANEPGVKAASRRGVNWLLSMQNRDGGWAAYDVDIDN
                     QVLTQLPFADHNAMLDPSCADITARVLELLGTLGYRADHPSVARALDYLWRTQEPEGC
                     WYGRWGVNYIYGTWQVLQGLKALDFPMEHPSLQRAAEWLESTQQPSGGWGETCQSYDD
                     PSLKGTGEATPSQTAWATLGLIAAGRAFSPAVRHGLDYLMETQSPDGSWDEPQFTGTG
                     FPRVFYLRYHLYRHYFPLMAMARYQSAIERRLGQSSPALACRIPAQPLSLEL"
BASE COUNT          491 a          838 c          796 g          540 t
ORIGIN      
        1 gatcgatttg cgcggaacac ttcagaatgc cggcgacctc gatcggctcc tggcaatttt
       61 gcgttcgatc ccgttactac aaggctttcg gctcgaaact gacttcaagg ccgactaacg
      121 acaccgtccg acggagttga tctcgctgcc gccgccgact gacccggctg cctcgcgcat
      181 cgagggacga tgctccacgt ctccgggcaa tagcgcttaa cactccttcg gcaactggcg
      241 ccgagccgcg tgcccgccat tcgtccgcgg tgtttcattc aatgctcgca aaataggcaa
      301 aatctagggg cggccttgtc tagaaatcat tcaaaatgct ctgggaacgg ggggtagttt
      361 gatcttgatc gaatacgtgg aacgcacgta ggatgagcgc cgcccgcagg taaggcaaac
      421 gtggtcaagc agttgttatt tgtcccttac ggttctgtca acctacagac gcctcgcaat
      481 gagggagagg gccgggaccg catgatcgcc aacacggacg ggggcgtctt gccaaatcat
      541 tcggaacggt tgatctctcg tcggtcggcg cacgccgcgc ttctcgctca cgacgacctc
      601 ggaagtgcgc tcgaacggac caaggactgg ctcctcttgc gtcagagcga ggagggtttc
      661 tgggtcgggg agctcgaggg cgacacgatc ctcgagtccg agtacatctt gctcttgacg
      721 tttctgggcc gcgagaccga tccggtctgc gtcaagtgtg cgcgttatat ccaggaccat
      781 cagttgagcg atggcggctg ggcgatttat cccggcggcc cgacggatat cagcgcttcg
      841 gtcaaagcct atttcgcgct caagctggtc ggcatatcac ccgatgacgc agcaatggtc
      901 agagcccgta gtacgatcct cgatgcgggc ggtgcccacc agtgcaacag cttcacccgt
      961 ttctatctgg ccctcttggg ccagatcagc tacgacgagt gcccatgtgt tccgccggaa
     1021 ctggtgttga tcccttcgcg tttgggcttc agcttgagtg cgatgtcggc gtggacgcga
     1081 acgatcgtcg tgccgctgtc gatcatggcg tattacaagc cggtgcggac actcgaaagt
     1141 gagcgcggca tcgccgagtt gttccgtagc gatcgtgggc aaccgtcgcg ccgcacggct
     1201 cgctggcttt cgtggaccaa cttttttttg attgtcgacc gggtactgaa gggcctcgac
     1261 cgctctattc ccgctctctg ccgtcggcct ggcatcgcgg cggcgcaccg ctggatgctc
     1321 gagcactgcg aaaacaccga cggactgggc gcgattttcc ccccgatggt ctattcaatc
     1381 attgcgctac gatgcctggg ctacgagatg ggttcgccgg ccgtcctatg ggcgatgaag
     1441 cagctcgacg acttgcacat cgcccaagga gatcgggttc gcgtgcagcc ttgcctttcg
     1501 cccgtgtggg acacagccat cgccatgatc gcgctggccg acgctcaggt acccgccgac
     1561 catccctcct gggcacccgc ggtcgagtgg ctgctctcga aggaagtgag aaatcccgga
     1621 gattggtgca tcagaggtcc ccgcgtggaa ccaaccggct ggcacttcca gttccataat
     1681 ggattttatc cggatctcga cgacagcgcg atggtgatcc ttgcgctcaa tcgctccccg
     1741 ctagcaaacg agccgggtgt caaagccgca agccgacgcg gggtcaactg gctgctttcg
     1801 atgcaaaacc gcgacggcgg ctgggcggcc tacgacgtcg atatcgacaa tcaggtcctc
     1861 acccagctcc cgttcgccga tcacaatgcc atgctcgacc cgagctgcgc ggatatcacg
     1921 gccagggtgc tcgagttact cggcacgctc ggctatcgag ccgatcaccc gtcggtcgcc
     1981 cgcgctctcg actacctgtg gcgtacgcag gaaccggaag gctgttggta cggccgctgg
     2041 ggggtcaact acatctacgg aacctggcaa gtcttgcagg gcttgaaagc ccttgatttc
     2101 cccatggaac acccctcgct ccagcgggcc gccgaatggc tcgagtcgac ccagcagccc
     2161 tcgggcggct ggggcgagac ctgccagagc tacgacgacc catccttgaa ggggactggc
     2221 gaggcgactc cctcacagac ggcttgggcc accctcggtc tgatcgccgc cggccgcgcc
     2281 ttctcgcccg ccgttcgtca cggtctcgat taccttatgg agacccagtc gcctgacggc
     2341 tcatgggatg aaccgcaatt taccggtacg ggatttccca gagttttcta tcttcgctat
     2401 cacctctatc gccactattt tccgctcatg gcaatggccc gataccaatc ggccatcgaa
     2461 cgccggctgg gccagtcgag cccggcgctg gcatgtcgga tcccggcaca acccttgtcg
     2521 ctcgaattgt gagaaggagt cccggcgatc aacccgaccg tggtcaccgg ccgcggtcga
     2581 gtaggtccga gagaagaagg atgaagcccg gcaagacgtc accgccgtcg agcgcctgat
     2641 ccgcgcggat gagcactggc ttttc
//
LOCUS       DATAQL010001165         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1035868, whole genome shotgun sequence.
ACCESSION   DATAQL010001165 DATAQL010000000
VERSION     DATAQL010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035868"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VHS97_20100"
     CDS             complement(<1..309)
                     /locus_tag="VHS97_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX3450568.1"
                     /translation="MTFARPELLWLLMFLPGMLIWAIRGRIQRRRDWQALAQRGRVPR
                     DGTLFIVGCVACAIIAMAQPRWGRLAASPLPPGHDVVLLVDVSRSMAVEDAVPNRLAA
                     A"
     gene            641..>1703
                     /locus_tag="VHS97_20105"
     CDS             641..>1703
                     /locus_tag="VHS97_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450569.1"
                     /translation="METSSDSRDRAGDPRRTQSTGQKDGLHDATRALLAQPVRGFARF
                     LYSSNPFYILSADLVFVGLRISFGSGGPASYSWALAIGLATYTLLLATTACFLIRVGK
                     LWDDLRSLLLLIVILFLAMAMSFDDTMAADPRRGSLGYLGGFAFAVVVSEAVLRTIQL
                     RLAGWYRAAYHAILAVVFLYPIALAPVLGEPESPRLQWALFGFSPLAGLVLLLLVPAA
                     RGGVALVAKNGSPWRWPLYPWSLFFVMAGGVGVRCYSLCVSFHYVGGSRTIFGPYFLV
                     PMGLALSLIWLEIGIVARRRGVMVVASLMPLALAFMATTGFRYEPVYLHFLDLFRQTL
                     GGSPVFWALVGAAAFHAYAA"
BASE COUNT          260 a          522 c          517 g          404 t
ORIGIN      
        1 cgccgcggcc agccgattgg gaaccgcgtc ttcgactgcc atgctccgac tgacgtcgac
       61 caggaggaca acatcgtgcc caggcggtag tggcgacgca gccaaccgtc cccaccgcgg
      121 ctgagccatg gcgatgatcg cgcacgccac gcaccccacg atgaataagg tgccatcgcg
      181 cggcacccgg ccgcgctggg cgagcgcctg ccagtcccgc cttcgctgga ttcgcccccg
      241 aatcgcccag atgagcatcc cgggcaggaa catgagcagc cacaacaatt caggtcgtgc
      301 aaaagtcaag gttgagcctc gagagacccg tcgtcatcaa tataagcata agctcgaccg
      361 tcgaaatctc gatcgtcgat cgatccatta tgaccgatcg cacatccgtt tcgcagccgg
      421 aattcctcgt atcgactgcg aaccggcgag atcgcctgcg attatcgtgt cacgtcttgc
      481 gacaattgtc gtagagattg atagtcagtt tttgcatcgc gctccattcg ggtcccatct
      541 tgtgcgagag gacaatcgtc ctcgggccgg tgccgggtcg cgacacgtcg cggaattgaa
      601 atcatctctc tatcgatttt ccaggtcgag gaggtgcgcg ttggagactt caagcgattc
      661 gcgggatcgc gctggcgacc cacgccgaac gcagtccacg gggcaaaaag atggattgca
      721 cgacgcaact cgcgctctct tggcacagcc ggtgcgaggc tttgcccggt ttttgtactc
      781 aagcaacccc ttctacattc tgagcgccga cttggtgttt gtgggtctga ggatctcgtt
      841 cggctcgggc gggccggctt cttactcgtg ggcgcttgca atcgggttgg cgacttatac
      901 cttgctcctg gctacgacgg cttgtttcct gattcgcgtc ggtaagctct gggacgacct
      961 gcgcagcttg ctgctcttga tcgtgatctt gtttctggcg atggcgatga gctttgacga
     1021 cacgatggca gccgacccgc gccgtggttc actgggttac ctcggcgggt ttgcgttcgc
     1081 ggtcgtcgtt agcgaggcgg ttttgcgtac gatccagctt cggctggccg gctggtatcg
     1141 tgccgcgtat cacgcgatcc tggccgtagt gttcctttat ccgatcgcgc tggccccggt
     1201 tttgggcgag cccgaaagtc cgcgactcca gtgggcactc ttcggttttt cgccgctggc
     1261 gggtctggtg ttattactcc tggtgccagc ggctcgcggc ggagtcgcac ttgtcgccaa
     1321 gaacggcagc ccctggcgct ggccgctcta tccctggtcg ctcttcttcg tgatggccgg
     1381 cggcgtcggc gttcggtgtt actcactttg cgtctcattt cactatgtgg gaggcagccg
     1441 cacgatcttt ggcccgtact tcctggtccc gatgggtttg gccctttccc tcatctggct
     1501 cgagatcggc atcgtcgcgc ggcgccgtgg cgtgatggtc gttgcctcgt taatgccgct
     1561 tgcgctcgct ttcatggcga cgaccggctt tcgttacgag ccggtgtatt tgcactttct
     1621 tgacttgttc cgccagaccc tgggtgggtc gcccgttttc tgggcgctcg tcggtgcggc
     1681 ggcattccac gcatatgcgg cca
//
LOCUS       DATAQL010001166         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5003381, whole genome shotgun sequence.
ACCESSION   DATAQL010001166 DATAQL010000000
VERSION     DATAQL010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5003381"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VHS97_20110"
     CDS             <1..645
                     /locus_tag="VHS97_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450570.1"
                     /translation="KTITVKSTRTSFANLRRKAMGVPSPWDDTSDNYLDDGLSKPFSR
                     AHQGPNRRQLLVEGINDKVKFWSALVDNASKVEGMRPQPPMTGYFREFMALAREHGVH
                     VYVGLLPVHPDYEQRVFTPRLREIREELSALLRDTCREFECEYRDFSSLKSFDGDPED
                     FIDGTHPTPRNARRMIDELLRHRPDRLTASRSAMRHDRPQRANSLRTSLASACD"
     gene            1009..1755
                     /locus_tag="VHS97_20115"
     CDS             1009..1755
                     /locus_tag="VHS97_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX3450571.1"
                     /translation="MARIISVANQKGGVGKTTTAINIAAGLAKSGRKTLVVDVDPQCN
                     ATSGLGVTPAARHPLVAGQPLAEAVVETKQPGLFVLPGSQSLADADALSASNRQRAAA
                     LRQQISGELGRFDYVFFDCPPSLGQLTRAALGTSGEIFIPIQCEYFAMEGLSQIIELA
                     RQTKARDNNRLEIGGIVLTMFDPALELANEVADEVRQYFAETVFESLIPRDVAISEAP
                     SHGLSVLDYSPRARGARAYTELVMEVIDRE"
     gene            1748..2653
                     /locus_tag="VHS97_20120"
     CDS             1748..2653
                     /locus_tag="VHS97_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3450572.1"
                     /translation="MNKRRLGRGLEALLGREEGGFEGGALDTSDMMQIAVDQIDANPF
                     QPRRQFNPDEIASLADSLRQHGMLQPVLVRAVGDRYQLIAGERRLRASIEARLHELPA
                     RVLVLDDQHVFEIAMVENLQREDLNAIDKATAFREYLTRYGVTQEELAGRLGLDRSTI
                     SNLIRLLELPDQVLDAVRAQEISQGHARALLGLEDASSRVEACQRIVAEHLSVRQAEA
                     LVAMGAPSPARPRLRKDAPHAHDARPPHLLDLEQHLNQRLGTTVLIRAKNRDRGQIII
                     DYHSRDEFDRLASLFQEASETVSSS"
     gene            2946..3695
                     /locus_tag="VHS97_20125"
     CDS             2946..3695
                     /locus_tag="VHS97_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450573.1"
                     /translation="MSPQPHECVAEDELSCSILRLVDDPERVESLRRMLSSFNHQCRN
                     SLNGIKMSIYLFKREAGGPMPQRWLELERTYQELERLFDRLQVIYRPLTLTLVRSPFG
                     QLVSERLPTWRSWFNLRGRALEVAPPGDDGPSDFDPMYLGLALDAFVAWRAEAGYANF
                     QTSLSWKIEGDTLEVRCDEARPAALCQAAERQRAGAHDSRPLGQIDSLALPLLARIVS
                     AHGGYLETSSGPDFTMRLRFPRFQSGEQSSR"
BASE COUNT          749 a         1146 c         1118 g          754 t
ORIGIN      
        1 aagacaatta cggttaaatc gacgaggacg agcttcgcca atctcaggcg caaggcgatg
       61 ggagtaccct cgccatggga tgacacctca gacaattacc ttgacgacgg tctgagcaag
      121 ccgttctcca gagcccacca aggaccgaac cgtcgacaat tgctggtgga aggcatcaac
      181 gataaagtga agttctggtc ggctctcgtc gacaacgctt cgaaggtaga gggcatgagg
      241 ccacagccgc caatgaccgg ttattttcgc gagtttatgg ctttggcccg cgaacacggc
      301 gtccacgtct atgtcggcct gttgcccgtt caccccgatt atgaacaacg ggtcttcact
      361 ccgcggttgc gcgagatccg ggaagagttg agcgctctgc ttcgtgacac gtgtcgagaa
      421 ttcgagtgcg aataccgcga cttttcgtcg ttgaaatcgt ttgatgggga tccggaagac
      481 ttcatcgatg ggacgcaccc cacgccgcgg aatgcgcggc ggatgatcga cgagttgcta
      541 aggcatcggc ccgatagact caccgcctct cgatccgcca tgcgacacga ccgtcctcag
      601 cgagcgaact cgttgagaac cagcctcgct tcagcgtgcg attaacaacg tggttgctgt
      661 tgcccatact gatcgcctcg gcctcggatg acgccgtcga cccaaggtgt tagactccgc
      721 gctcgggctc tactgtccgc aacattcgcc agcgaaacct atcgtgcggt tgagtgtgtc
      781 cgaacgcgcg aacactacgc tcgatcaatt ctactttaca tagtctcgcc gactcgctac
      841 gattccggcc ttgcggcagt cggtggaatc tggccatctc gcgcgacgcg accaaggtcg
      901 ataagctggt ggccggggga tggtcgagcc gtaacgcact gatgcgccat ccgcgtcaac
      961 ctggacgctg ggcccaaccg ggcgcgacac gcggaggcga agaatacgat ggccagaatc
     1021 atcagcgtgg ccaatcaaaa ggggggcgtc ggcaagacca ccacagcgat caacatcgcc
     1081 gcggggctcg ccaagtccgg acgcaagacc ctggtggtcg acgtcgaccc ccaatgtaac
     1141 gcgaccagcg gtctaggggt tacgccggcc gcgcgtcatc cgctggttgc gggacagccg
     1201 ttggctgaag cggtcgtgga aaccaagcaa cctgggctgt tcgtattacc cggctcgcaa
     1261 agtttagccg acgccgatgc gctgtcggcc tccaatcgcc aacgcgccgc cgcgttgcgc
     1321 caacaaatta gcggcgagct cggccggttc gactatgtgt tttttgattg ccctccctcg
     1381 cttggtcagc taacccgcgc ggccctcggc acttcgggcg agatcttcat cccgatccaa
     1441 tgcgaatact ttgccatgga ggggctctct caaatcatcg agctggcgcg tcaaaccaag
     1501 gcaagggata acaatcggct cgagattggc ggcatcgtgc tcaccatgtt cgatccggca
     1561 cttgagcttg ccaacgaagt ggccgatgaa gttcgtcagt acttcgccga aaccgtcttc
     1621 gagtcgctga tacctcgaga cgtggcgatc agcgaagctc ccagccatgg cctgagtgtt
     1681 cttgactact cgccgcgggc tcgaggcgca cgagcgtata ccgaacttgt catggaggtc
     1741 atcgaccgtg aataagcgac gcttggggcg cggactcgaa gcactgctcg gccgtgagga
     1801 aggtggattt gaaggggggg cgcttgacac gagcgacatg atgcagatcg ccgtcgatca
     1861 aattgacgct aacccctttc agccgcgccg gcagttcaat cccgacgaaa tcgcctcgct
     1921 ggccgattcg ttgcgacagc atggcatgtt gcagccggtg ctcgttcgcg cggtcggcga
     1981 tcgctatcag ctcatcgccg gtgaacggcg gctgcgggca agtatcgaag cacggctcca
     2041 cgaattgccg gctcgggtcc tcgtgctcga cgatcagcac gtcttcgaaa tcgcaatggt
     2101 cgaaaacctg cagcgcgaag acttgaatgc catcgacaag gccaccgcgt ttcgcgagta
     2161 cctgactcgc tacggtgtca cccaggaaga gctggcaggc cgactgggtc tcgatcggtc
     2221 gacgatctcc aacctgattc ggcttctcga gcttcccgat caggtcctgg atgcggttcg
     2281 cgcccaagag atctcccagg gacacgccag agccttgctc ggcctggaag atgccagcag
     2341 ccgtgtcgaa gcttgccagc gcatcgttgc cgaacacctc tcggttcgcc aagccgaggc
     2401 actggtcgcc atgggcgcgc cgtcacccgc gcggcccagg ctccgcaaag acgctccgca
     2461 cgcgcacgac gccaggcccc cccatcttct cgatctcgag cagcatctca atcaacgcct
     2521 gggcacgacg gttttgatcc gagccaagaa ccgggatcgg ggccagatca tcatcgacta
     2581 tcactcgcgc gacgagttcg atcgtctggc gtcgctattt caagaagcca gcgagacggt
     2641 gtccagttcg tgattctcgt catcaagcca atggtgcgcg ccaacaagcg agttcgcttc
     2701 ccgtttcgcg ctcaatgttc ggagtaggag aatgttggtt cacgatttat tgatcgggtc
     2761 ggccgcagcc aacgtctttg acttattttt acttctttgc tcagagaacg gttgcgtcgt
     2821 tcgcgctcta gaagggctcg ccgatcggca ctaggtttgc ctctgaaatt ctacgtaagc
     2881 cccccagccg accatgcacg cgcgtggaaa gttcgccgaa aatccgccaa attaggtatc
     2941 gccccatgag tccgcaaccg cacgagtgcg ttgcggagga tgagctttca tgctcgatcc
     3001 tccgcttggt tgatgatccc gagcgggtcg aatcgctccg aaggatgctg agcagcttca
     3061 atcaccaatg ccgtaactcg ttaaacggca tcaagatgag tatctatctc ttcaagcgcg
     3121 aggccggcgg gcccatgccc cagcgctggc tcgagcttga gcgcacgtat caagagctcg
     3181 agcggctgtt cgatcggctc caggtgattt accggcctct gacgttgact ttggtacgtt
     3241 cgcccttcgg ccagcttgtg agtgagcgcc tccccacctg gcggtcttgg ttcaacctcc
     3301 ggggacgtgc actcgaggtc gccccgcctg gcgacgacgg tccgagcgac tttgatccca
     3361 tgtacttggg gctcgcgctt gacgcgttcg tcgcctggag ggcggaggca ggttatgcca
     3421 actttcaaac cagcttgagc tggaaaatcg aaggtgacac gctcgaagtc aggtgcgacg
     3481 aagcccggcc ggcggcattg tgccaggccg ccgaacggca gcgtgcgggc gctcacgact
     3541 cgagaccgct gggacagatc gattcactgg cattgcctct cttggcgcgg attgtctcgg
     3601 ctcatggagg atacctggaa accagcagcg ggcccgattt cacgatgagg ctccgcttcc
     3661 cgcggtttca aagcggcgag cagtctagcc gatgatctcc cgtgggtggc aatggcaacg
     3721 cagctcgggt acccgtggta gcgtgaagta ggtcgggctt tccagcc
//
LOCUS       DATAQL010001167         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2411283, whole genome shotgun sequence.
ACCESSION   DATAQL010001167 DATAQL010000000
VERSION     DATAQL010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411283"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VHS97_20130"
     CDS             complement(<1..657)
                     /locus_tag="VHS97_20130"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450574.1"
                     /translation="MNNGRRRPKLLRANLRPADSGLINEGRVAKQRTRATLLGLVMVS
                     LVLCAVIVHGSGRPFTYRIGQRTDRELRVNVKEFRIRNQTKTSNERQAAADQVPPLLI
                     NDPAPIKELSARLDDLTTAVAKSTRFEEMNENVRAVWKLNPESYLDLKAATDTPERRD
                     NLHVQIAAAFAALLRDGVLGPGTLPPNEESSRLLSVRTADQPRSLARVVPRDRVVPER
                     I"
     gene            complement(820..1839)
                     /locus_tag="VHS97_20135"
     CDS             complement(820..1839)
                     /locus_tag="VHS97_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX3450575.1"
                     /translation="MPEVSISLDGHAEELGVFGSRDQYLRQIRDALGVKVLARHGEVR
                     IEGDADRIEQARRIFEGLRSIFRARKSISSNDVSELIDVTLNCSEPDQPNLHEIREGN
                     RVVRPRSPGQARYMKALFENELVFCIGPAGTGKTYLAVAMAVSLLRRGRIKKIVLVRP
                     AVEAGEHLGFLPGDLEAKINPYLRPLLDALHDLMDYDQIRRYMDNDLIEIAPLAYMRG
                     RTLNDAAIILDEGQNATVPQMKMFLTRMGQNARIVVTGDITQVDLPPGTKCGLADAVD
                     RLKQIPGLATTLLDRSDIVRHPLVQAIVDAYEISEPTAEREGRPEAVPVEPMVPLPAS
                     KTAPE"
     gene            complement(2160..>2619)
                     /locus_tag="VHS97_20140"
     CDS             complement(2160..>2619)
                     /locus_tag="VHS97_20140"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX3450576.1"
                     /translation="FTLQLRWFEGRLQGIVALVFLVATAKGADTGAYAMGRLAGRHQL
                     WPSLSPKKTVEGAVGGLLFGVAAALIVAAIARYILKIPTLEWMVAALYGLVIGAVAQL
                     GDLMESMIKRDCERKDASAVVPGFGGVLDVIDSLIFAGPVAYLFWLWWGP"
BASE COUNT          535 a          805 c          789 g          490 t
ORIGIN      
        1 aatccgttcg ggaaccacgc ggtcgcgtgg taccacccga gcaagggacc gtggctggtc
       61 cgccgtgcgc accgacagca gccgactcga ttcttcgtta ggaggcaagg ttcccggccc
      121 gagcacaccg tcgcgaagca aagctgcaaa agcggcggca atctgaacgt gcaagttgtc
      181 gcggcgctcg ggggtgtcgg tggctgcctt gaggtcgaga taactctcgg gattgagttt
      241 ccagacggct cgcacgtttt cgttcatttc ttcgaaacgt gtcgacttcg ccacggcagt
      301 ggtcaggtcg tcgagccgtg cggagagttc cttgatcgga gccggatcgt tgatcaatag
      361 cggcgggacc tgatcggcgg ccgcctgccg ctcgttgctg gtcttggttt ggttgcggat
      421 cctgaattcc ttgacgttga cccgcagctc gcggtcggtc cgctggccaa tccggtaggt
      481 gaatgggcga ccggagccat gaacgatgac cgcgcacagg accagcgata ccatcacgag
      541 cccaagcagg gtcgcgcggg tacgctgctt ggcgactcga ccctcattga tcaaacctga
      601 atcggcgggg cgcagatttg cccgcaagag tttcggacgc cgtcggccgt tgttcattga
      661 ttgttctcgg cacggcgtgg tcgaggagga ccctcgtccc acgcggaagt gcgcctggac
      721 cacgcggccc cggcttgacg gtccgtccta atcgctcggt tctttctcat ggcgatcatg
      781 aatcgaacag cacgaaaaaa ggacggcaac gcgacgccct cactccgggg cggttttgct
      841 ggcgggaaga ggaaccatcg gctcgaccgg gacggcttca ggtcgccctt cgcgttcggc
      901 ggtcggctcg ctaatctcat aggcatccac gatagcctgc accaagggat gccggacaat
      961 atcggagcga tcgagcaggg ttgtcgccaa ccccgggatc tgtttgagtc gatcgaccgc
     1021 gtcggccagg ccgcatttgg ttcccggcgg caggtcaacc tgcgtgatgt cgccggtcac
     1081 cacgatccga gcgttttggc ccatacgcgt caagaacatc ttcatttgcg ggaccgtcgc
     1141 attttggccc tcatcgagga tgatcgcagc atcgttgagc gtacgcccgc gcatatacgc
     1201 gagcggggcg atctcgatga ggtcgttgtc catgtagcgc cggatctggt cgtaatccat
     1261 caggtcgtgg agcgcgtcca agagcgggcg caagtacggg ttgatcttgg cctcgagatc
     1321 gcccggcaag aaaccgaggt gctcacccgc ctcgaccgcc ggccgcacca gtacgatctt
     1381 tttgatgcgg ccccggcgca aaagcgacac cgccatcgcg accgcgagat atgtcttccc
     1441 cgtgccagcc ggtccgatgc aaaagaccag ctcattctcg aacagtgcct tcatataccg
     1501 agcctgcccc ggggaacgag ggcggactac gcgattgcct tcgcgaattt cgtgcaggtt
     1561 cggctgatcg ggctcggaac agttgagcgt gacatcgatc aactcgctga cgtcgttgga
     1621 cgagatcgac ttgcgagcac ggaaaatcga ccttaatccc tcgaagatcc gccgcgcttg
     1681 ctcgatgcga tcggcgtcac cttcgatccg gacctcgcca tgccgggcga gaaccttgac
     1741 tccgagcgcg tcgcgaatct gcctgagata ctgatcacgg ctgccgaaga cacccagttc
     1801 ctcggcgtga ccatccagac tgattgagac ttccggcatg cctccccttt cgccgaccca
     1861 agtggagagc gacgcgccct cgactcggtc ggggtcatcg ctcaccaact cacacacggt
     1921 ttggggcggc ttgaacggat actattatgg aatcgaaaaa ccaaaaaatg caacatcgag
     1981 cccaccgaag tcaacgtaca gggacgttga cagcgtttac agccgaactc tcagcagaat
     2041 tgaaacttag gcttaaagat ccaacatttg tcctcgagcc gaaacgccgt atcttcagac
     2101 ggataggaca ttttggaaaa cctggccaag tcggccggaa acgaatcagc gacaggagat
     2161 caaggccccc accacagcca gaacagatag gcgaccggac ccgcgaatat gagcgaatcg
     2221 atcacgtcga gaacgccgcc aaagcccggg acgaccgcgg aggcgtcctt gcgttcgcag
     2281 tcgcgtttga tcatcgactc catcaggtcg ccaagctggg cgaccgctcc gatcaccagg
     2341 ccgtaaaggg cggcgaccat ccactcaagg gttgggattt tgaggatgta gcgggcgatc
     2401 gcggcgacaa tgagtgccgc cgcgacgccg aaaagcaggc ctcccaccgc tccttcgacg
     2461 gtcttttttg ggctcaacga cggccagagt tggtgccggc cagcgagacg gcccatggca
     2521 taagcgcctg tgtccgcgcc cttcgctgtc gccaccagaa acaccagggc gacgattccc
     2581 tgcagccgcc cctcaaacca gcgcagttga agcgtgaaa
//
LOCUS       DATAQL010001168         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4545942, whole genome shotgun sequence.
ACCESSION   DATAQL010001168 DATAQL010000000
VERSION     DATAQL010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4545942"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1497
                     /locus_tag="VHS97_20145"
     CDS             232..1497
                     /locus_tag="VHS97_20145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3450577.1"
                     /translation="MDLQSFRRAGHMPTLMCAFLYFTVSCMAWMMVGALATSIIADLG
                     VLSDSHKGLLVAVPVLGGAILRLVLGPMADYMGAKKTALLGLVLTLVPLVLGWLWSVS
                     YLQVLLVGLLLGVAGASFAVALPLASRWYPPQYQGLALGIAGAGNVGTALATFFGPWV
                     ASLLGWHAAFGIALVPVVATLALVALVAQDSPTRPAPKTLADYAAVLKIVDTGWFCFF
                     YAITFGGFVGLVSFLGIFFHDQYALTKVQAGFFATLCALTGSMIRPLGGYVSDRFGGV
                     RVLTWIYLGVAVMMAGVSSLPPLGLAILLHVTGMSLLGMGNGAVFQIVPQRFPREIGV
                     ITGLVGAAGGLGGFVLANVLGSLRQLTESHGAGFLAFGLASAMGAGAIFLVGRRWEST
                     FLGKGGVVVDRQAPAPVAAPAASVELTWV"
BASE COUNT          238 a          495 c          548 g          368 t
ORIGIN      
        1 aatccgggat tgtcgcgcgg cgtcgtcttc gtcccgtttc attggggcga tcaacaccga
       61 gagaacgtgg cagcgaatta cttgacgatc ccggcaatcg gctggatcgc caagcagccg
      121 gaattcaagc attgcgcggt ccggctggca ccagcgttcg aggtcccgta ggccggcgtg
      181 gcctccattc caccgcgatc gcatactaga acccgaaccg ggagtcagcc gatggatctc
      241 caatcgttcc gacgcgccgg ccacatgccg acgctcatgt gtgcattttt atatttcacg
      301 gtgagttgca tggcctggat gatggtcggc gctctggcta cctcgatcat cgccgacctc
      361 ggagtgcttt ccgactcgca taagggtctc ttggtcgcgg tcccggtgct cggcggggcg
      421 atcttgcgcc tggtcttggg ccccatggcc gactacatgg gcgccaagaa gacggcgctc
      481 ttggggctag tgctgacgct cgtcccgctc gtcttgggct ggctctggtc ggtcagttac
      541 ttgcaagtcc tgctggttgg cctgttgctg ggcgtggccg gggcgagctt tgcagtggcg
      601 ctgcccctgg cgagccgctg gtatccgccg caataccagg ggctggccct ggggatcgcc
      661 ggagcgggta atgtgggcac ggcgctagcc acattcttcg gcccctgggt tgcgtctctc
      721 ttgggctggc atgcggcgtt cggaatcgcg ctggtgccgg tggttgcgac cctggcctta
      781 gtcgcgctcg tcgcccagga cagtccgacg cgacccgctc ccaagacgct tgccgactac
      841 gccgccgtgc tcaagatcgt cgacaccgga tggttctgct tcttttatgc catcactttc
      901 ggtggtttcg tggggctggt aagctttttg ggcatttttt tccacgacca gtatgcactg
      961 acgaaggtcc aggccggttt tttcgcgacg ctctgcgcct tgaccggctc gatgatccgc
     1021 cccttgggcg gctacgtgtc cgatcgattc ggcggcgtcc gggtgctgac ctggatctat
     1081 ctcggcgtcg cggtgatgat ggcgggcgtt tcgtccctgc ccccgctggg cttagctatc
     1141 ctcctgcacg tgacaggcat gtcgctcttg ggtatgggca acggcgccgt cttccagata
     1201 gtccctcagc gattcccgcg agagatcggc gtgatcaccg ggctcgtcgg ggccgcgggg
     1261 gggctcgggg gattcgtact ggcgaacgtc ttgggcagct tgcgtcaatt gacagagagc
     1321 cacggcgccg gcttcctggc gttcgggctg gctagcgcga tgggcgcggg tgccattttc
     1381 ttggtaggca ggagatggga gagcacattc ctcggcaaag gcggtgtggt ggtcgaccgg
     1441 caagcaccgg cacccgtcgc ggcgcctgcg gcgtctgtcg agcttacctg ggtctaggtt
     1501 tccgggtcga aagtcgagat cggcaagaaa acccccctgg aaggtgaccc tcctatcgac
     1561 ggcagttcga attcggtaaa gagtacttcg ctaagctcgt gccgctcgag cgtctcggct
     1621 ctccggaaga aatcgccaat gtggtggcg
//
LOCUS       DATAQL010001169         5098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1036263, whole genome shotgun sequence.
ACCESSION   DATAQL010001169 DATAQL010000000
VERSION     DATAQL010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036263"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VHS97_20150"
     CDS             <1..222
                     /locus_tag="VHS97_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450578.1"
                     /translation="GYSGHTPHSWHGFFTIDTDPEVDVEDTLADWEQSQGLLPNHVQW
                     IGADPARTSQCETRRASPGDLSTPLLGSM"
     gene            complement(308..2239)
                     /locus_tag="VHS97_20155"
     CDS             complement(308..2239)
                     /locus_tag="VHS97_20155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3450579.1"
                     /translation="MATILLAISFGICAGYLDVGAVVIKKYYFNSEGHFRNARDFPWT
                     LPVGHAVLMMVPGVLVAAVSRFRPVPISLRAASWIFATLAISSALLRMPLYSTCSFLL
                     AAGLGHVISNAVAARGLGPRQVRSIFAMLIGVVGVTAALTSGRQAILEYRTVVGLSAP
                     PPTARNVMLIVWDTVRADNLGLYGYARDTTPNLAKWAQKGVTYKQALSPAPWTLPSHT
                     CFFTGQWPYRINSQLKLRLDASYPTLAERLASSGYQTAGFSANTDWCSYETGLDRGFA
                     HFEDFALTPRSLLSRTVAGQWILKSFVKLCSDFDTKWIHDMKWIDLQSRGASEINSDF
                     LQWLSRRRPDRPFFAFLNYFDAHQPYLPPPGYEGRFGIMPTKAADFQFLIDFRMSDKN
                     RSSALNLTMARDCYDDCIAYLDEQLGRLLDDLERQGILENTDVIITADHGEAFGAHHF
                     FGHDNTANLDEVGVPLVILSPAAPAGRVVNSPVSLRDLPATVVDLLGLSADSPFPGRS
                     LSAYWKLAPGAVPPDLTSPAFSEKFTEPVFEVASDEGRKTPGFQMSLVASGHHYVRDS
                     LGDEELYDLTTDPYERVNLAGPNSVYDTVAVFRRMLLDVLTNNPGSLEVEKAYLATYR
                     RWLEDLVRANSSRALAADD"
     gene            complement(2769..4217)
                     /locus_tag="VHS97_20160"
     CDS             complement(2769..4217)
                     /locus_tag="VHS97_20160"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3450580.1"
                     /translation="MKRTTAAILFCLTSAASGARAQEPSRSTEPVKAGPTTGSSSVSI
                     DDYIEGRMHKRHIPGASVAIVRDGKVVLAKGYGVANVELSVPATENTVYQLASVTKTF
                     TATAIMMLVEEGKLGLDDRIRARLPDLPAAWGEVTVRHLLNHTSGIKSYTAVRDFHKT
                     SRKDYTQGEILDLVAKEPPEFAPGEKWNYNNTGYFLLGMLIEKVTGKKYGEVLDERIF
                     KPLAMTQTRVNDLHAVIPNRAQGYTWGGKELRNGEYVSPTQPFSAGMLVSTVNDLVKW
                     DTALRSETLLKKSILEQMWTPTKLTKGGDAEYGFGWSIGKTNGHRSIAHGGGIPGFST
                     QLSRYIDDNLTVIVLTNADSGNAGALARGIAGRLVPALAEEAAKPIADTDPQTTERLK
                     GVILAAMKGEADPELFTQESKERHVAAIKQGKGTTASFGALREFRLEERKQSDQGVKL
                     RYRAIFENETMSAFFDLDKDGKIAGLGLQSAD"
     gene            complement(4236..4772)
                     /locus_tag="VHS97_20165"
     CDS             complement(4236..4772)
                     /locus_tag="VHS97_20165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEX3450581.1"
                     /translation="MLAGELYDPMDLELVAARNRARDLCQALNETREAQQEERRRIVR
                     ELFATGGDTVWMQPPFFCDYGSNIHLGERVFFNFNCIVLDVCRVTIGDYTLFGPAVQV
                     YTATHPMNAELRRKQEMCKPIEIGSDVWVGGGAIICPGVRIGSRSVIGAGSVVTRDIP
                     EGVFAAGNPCRIIREISE"
BASE COUNT          992 a         1507 c         1543 g         1056 t
ORIGIN      
        1 ggctattcgg gacacacacc ccattcgtgg catggatttt tcacaatcga caccgatcca
       61 gaagtggacg tggaggatac cctggccgat tgggagcaat cgcaaggcct cttgccgaat
      121 catgtccagt ggatcggagc cgatccggcc cgaacaagtc agtgcgaaac gcgacgggct
      181 tcgcctggtg atttgtcgac acctttactc ggctcaatgt aggtggtgga accggggacg
      241 caggattcaa attcgagtcc gcgtgcggcg tagtggccct gccggtaaga tcgcatgcgg
      301 acgcgattca atcatcggcg gcgagtgcgc gcgaggagtt cgctcgaacg aggtcttcga
      361 gccatctccg gtaggtcgcc aggtaggcct tttccacctc gagtgagcct gggttgttgg
      421 tcagcacgtc gaggagcatc cttcggaaga ccgccaccgt gtcgtaaacg gagttgggcc
      481 ccgccaggtt caccctttcg tacgggtcgg tcgtgagatc gtagagctcc tcgtccccca
      541 gactgtcgcg gacgtaatga tggccggagg ccaccaggga catctggaac ccaggtgttt
      601 tgcggccttc gtcactggct acttcgaaaa cgggctcggt gaatttttct gagaaagcag
      661 ggctggttag gtctgggggg accgcaccag gggccagttt ccagtaggct gacagcgagc
      721 ggcccgggaa cggcgagtcg gccgacagac ccagaaggtc gacgacggtt gcaggtaggt
      781 cgcgcaggct aacagggctg ttcaccactc gccccgccgg cgcggcgggc gaaaggatga
      841 ccagcgggac gccgacctcg tcaaggttcg ccgtgttatc gtggccgaaa aagtgatgag
      901 cgccgaacgc ttccccatga tccgcggtaa tgatcacgtc ggtattttca agaatccctt
      961 gccgctccag gtcgtcaagc aaacgaccga gctgctcatc caagtaggcg atgcaatcgt
     1021 catagcagtc gcgtgccatc gtgaggttaa gagccgatga tcggtttttg tcactcatcc
     1081 taaaatcgat gaggaactgg aaatccgcgg ccttcgtggg catgatcccg aaacgccctt
     1141 catatcccgg cggcggcaga tatggctggt gcgcatcgaa gtaattgaga aaggcgaaga
     1201 aaggacgatc tggccgtcgc cggctcagcc actggaggaa atcgctattg atctcgctcg
     1261 caccgcggga ttggaggtcg atccacttca tgtcgtgaat ccacttcgtg tcgaaatcgg
     1321 aacagagctt cacgaagctc ttcaagatcc actgcccggc gacggtacgg ctgaggaggg
     1381 aacgcggcgt cagcgcaaag tcctcgaaat gggcgaaacc ccgatcaagc ccggtctcgt
     1441 aactgcacca gtcggtgttc gccgagaacc cggcggtctg atagcccgat gacgccaggc
     1501 gctcggctag ggtcggatac gaggcgtcga gccgtaattt caactgtgag ttgatccgat
     1561 aaggccattg gccggtgaag aagcaggtat gcgagggcaa cgtccatggg gcgggcgaca
     1621 gcgcctgctt gtaggtaacg cctttttgtg cccacttcgc taggttcgga gtggtatcgc
     1681 gcgcatagcc atagaggccc aggttgtcgg cgcggaccgt gtcccagacg atcaacatga
     1741 cgttgcgcgc ggttggcggc ggggccgata atccgaccac cgttcggtat tcaagaatag
     1801 cctgccgacc cgacgtgagg gcggcagtaa caccaaccac tccgatgagc atcgcgaaga
     1861 tcgatcgcac ctgacgcggt cccaagccac gcgcagctac cgcgttgctg atcacatggc
     1921 ccaacccggc cgcgagcaaa aagctgcatg tactgtacag cggcattctg agcagggccg
     1981 acgagatcgc gagcgtcgcg aagatccacg acgccgcacg cagcgaaatg ggcacgggcc
     2041 ggaaccggct caccgcagcg accagcacgc ctggaaccat catgagaacc gcgtggccta
     2101 ccgggagagt ccagggaaaa tccctcgcgt tacgaaagtg tccttcgctg ttgaaatagt
     2161 atttcttgat gaccacggcc cccacgtcga gatagccggc gcaaatcccg aacgagatcg
     2221 ccagcaggat cgttgccatc gggctgagcg aagctcggcg tggggggcac cgaagcggga
     2281 tttcggccgg gttgctgttc atcggctagt agtccctgtc catcactgtt ctgggtctcc
     2341 actgaggaag acgtccgcgc aatcgctacg caactcgggt tcgcggcgca ccgcgatatt
     2401 ggcgggcgcg cgcggcctgc cgatcgcagc cccgtgtgtg ttctgaaccc ctacattatt
     2461 agggagcgtt ttttgagatc ggtttggcct gttcaactgg atgaggcgac cgaaaactcc
     2521 atgtgcgccc gctgtgttaa gaaatgttat gcgtcgccag ctggctcgga tggttgaagg
     2581 gtgacgcaac cccgacggga attctcgata tgtgcagaga aagattcaaa agtgcaggca
     2641 atgcaacttc gacgggcaac tgactttgtg cgccgtattc gagtgtttca aggggacgca
     2701 aacggcgaga gccggtcggg gcttcctcgc cctccgaccg gcatcgagag agaggctgct
     2761 tggggagact agtccgctga ttggagaccc aaacccgcga ttttgccatc cttgtcgaga
     2821 tcgaagaacg cgctcatcgt ctcattctca aagatggcgc ggtagcgtaa tttcacgcct
     2881 tggtcgcttt gcttccgctc ctcgagtcga aattcccgta gcgcaccgaa ggaagcggtt
     2941 gtgcctttgc cttgcttgat ggcggcaacg tggcgttcct ttgattcttg cgtgaagagc
     3001 tcgggatcgg cctctccctt cattgccgcc agaatcacgc ccttcaagcg ttcggtcgtc
     3061 tgcggatcgg tatcggcgat cggctttgcc gcttcctccg ccagtgccgg aaccagccgg
     3121 ccggcgattc cccgcgcgag cgcacctgcg ttgccgctgt ctgcgttggt caagacgatg
     3181 acggtcagat tgtcgtcaat gtagcgcgag agctgggtag agaagccggg gataccaccg
     3241 ccatgcgcga tcgatcgatg cccattcgtt tttccgatcg accagccgaa accgtattct
     3301 gcatctccgc ccttggtcag tttggtcggc gtccacatct gttccaagat gctctttttg
     3361 agtagcgttt ccgaacggag cgcggtgtcc cacttcacca ggtcgttgac agtcgagacc
     3421 agcatgcccg ccgagaacgg ctgggtcggg ctgacgtatt ccccattcct gagttctttg
     3481 ccgccccagg tatagccctg cgcgcggttc gggatcacgg cgtggaggtc gttcacgcgg
     3541 gtttgcgtca tggcgagcgg cttaaatatg cgctcgtcga ggacctcgcc gtatttcttg
     3601 ccggtcacct tctcgatgag catcccgagc aggaagtacc ccgtattgtt gtagttccac
     3661 ttttcgcccg gcgcaaattc cggcggctct ttcgcgacca ggtcgaggat ctcgccctgg
     3721 gtgtagtctt tcctcgacgt cttgtggaag tcgcggacgg ccgtatagct cttgattccg
     3781 gaggtgtggt tcagcagatg acgaacggtt acctctcccc aggccgccgg caggtccggc
     3841 agtcgcgccc taatcctgtc gtcgagtccc agtttgccct cctccacgag catcatgatc
     3901 gcggtcgcgg tgaacgtctt ggtcaccgag gcgagctgat agacggtatt ctcggttgcc
     3961 ggcaccgaaa gttcgacgtt cgccacgcca tatcctttcg caagaaccac cttgccgtcc
     4021 cgcacaatgg cgacggaggc gcccgggata tggcgcttgt gcattcgacc ctcgatatag
     4081 tcgtcgatgc tgacgctcga ggagccggtc gtcggcccgg ccttgaccgg ttcggtcgac
     4141 cgcgagggct cctgggcacg agcgcctgac gcggcggatg tcaggcagaa gaggatcgcg
     4201 gccgttgtgc gtttcatggc gaaatctgac tccttctatt cggaaatctc gcggattatt
     4261 cggcagggat tgccggccgc gaacacgccc tccgggatgt cacgcgtgac cacactgccc
     4321 gcgccgatca ccgaccggga accgatccga acaccagggc aaatgatcgc tcccccgccg
     4381 acccacacgt cggagccgat ctcgatcggc ttgcacattt cctgtttccg ccgcagctcg
     4441 gcattcatcg ggtgcgtggc cgtgtagacc tgaaccgccg gcccaaacag cgtgtagtcc
     4501 ccaatcgtca cccggcacac gtcgagcacg atgcagttga agttaaagaa cacgcgttcg
     4561 ccgaggtgaa tatttgaacc atagtcgcag aagaacgggg gctgcatcca gacggtgtcg
     4621 ccccctgttg cgaacagctc gcgcacgatc cgtcgccgct cttcctgctg tgcctcgcgg
     4681 gtctcgttga gcgcctgaca caggtctcgc gcccgattcc gagcggcgac gagctcgagg
     4741 tccatcgggt cataaagctc gccggccagc atcttgtcgc gttcactctg cattcttcct
     4801 ccagccaaga taactccgca gacttacagt cgttcgtgtc aaaagtacca ttttttggtg
     4861 acggccgcag ctattgcacc gctgcggtcg aagtccatgt cctggattcg atgttcagct
     4921 tcttcacaat gaggcgtcgc ctgctcgcta gcctcttggc cggtcgttca ctgaaaaagt
     4981 tagctcaacc tgttgaccgg tcaccttttc gcaattagga tgcacctaac ttattaagtt
     5041 aggcggccga cctctaggtc cggctgagga ggtgagtttg accgaaaacg aaccgagt
//
LOCUS       DATAQL010001170         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1339699, whole genome shotgun sequence.
ACCESSION   DATAQL010001170 DATAQL010000000
VERSION     DATAQL010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339699"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..>1685)
                     /locus_tag="VHS97_20170"
     CDS             complement(256..>1685)
                     /locus_tag="VHS97_20170"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX3450582.1"
                     /translation="KPSDSFPGLLAFATGGSPISHGVFYDDTYDATLYPPGSNCTGTP
                     GTEVSNFEALDWDLTKLDGGSPPPGAEFDPRNPHINPAHLALRKTASGCEKVWPHLYM
                     KNKTNTIFEIIHQAGLRTAWSDKHPAYEILNGPSGLGLDELYAPEINSTSLQANGFPS
                     APASADWTFDPIYTRAYDGFKVKVVLNWINGLDQTGTKTVGVPAIFGMNFQAVSVGQK
                     VTVAMEGSTTTRGGYVDAAANPTQPLTQSLDFVDASLGQMYEALESRHLLKSTLFIVG
                     AKHGQSPIDVTKLHMLKGSTNAYATADVSDPAVVLGNGGVQVAQETADDVSLLWLKKH
                     SDVTAAVSILDADQATGNSTHIQTLYHDGALKQMWGDPAGGRVPDLIIQPIHGTIYSK
                     SAAKLAEHGGLSADDTNTLLVVSNPRLEKKVVDTPVTNMQVAPTILKALGLDPDSLVD
                     VKREGTKVLPGLESIGADDRGAHSE"
BASE COUNT          301 a          512 c          521 g          351 t
ORIGIN      
        1 gctcatgatc ggacgttctc gcggctactg cgagtgacct tggcctcttc agtacagtac
       61 gcggccattc gacaagtgac gaacccgatg ggatgcgacc gaccgcaaag gcctggccta
      121 gatttcccgt attgcgagct caaggtggcg aattattggg ctcaatgagc atcctcgtcc
      181 tgacgccagc cctctcagta acgcaccacg ccagcggcct gaagctatag gtgaccgctg
      241 gcgctttcac tgccgtcact ccgaatgggc acccctatcg tcggcgccaa tgctctcgag
      301 gcccggaagg accttggtgc cttcacgctt aacgtccacc aggctgtccg ggtcgaggcc
      361 gagcgccttc agaatggtcg gcgccacttg catattggtc accggcgtgt cgacaacctt
      421 cttttccagc cgcggattgg agaccaccaa cagtgtgttc gtatcgtcag cgctaaggcc
      481 gccgtgctcg gccagctttg ccgcgctctt gctgtaaatc gtcccatgta tcggctggat
      541 gatgagatca ggaacacggc cccctgcggg atcgccccac atctgcttca aggctccgtc
      601 gtgatagagc gtctggatgt gggtggaatt gccggtcgcc tggtcagcgt cgaggatcga
      661 taccgccgcg gtcacatcgc tgtgcttttt gagccatagg agcgacacat cgtcggccgt
      721 ttcctgggcc acctgtacgc cgccgttgcc cagcacgact gcggggtcgc tcacgtcggc
      781 ggtagcataa gcgttcgtcg agcccttcag catgtgaagc ttcgtgacgt cgatcggtga
      841 ctggccatgc ttggcaccga cgatgaacag cgtcgacttc aacagatgcc tggattccag
      901 cgcctcgtac atctgcccca gggaggcatc gacgaaatcc agcgactgcg tcaacggctg
      961 cgtggggtta gcagctgcat cgacatagcc accacgggtc gtggtgctac cctccatcgc
     1021 caccgtgacc ttctgtccga cgctaaccgc ctggaaattc atgccgaaga tcgcgggcac
     1081 gccgaccgtc ttggtgccag tctggtccag gccattgatc cagttcagga cgactttgac
     1141 cttgaagccg tcgtaggccc gggtatagat cgggtcgaac gtccagtcgg cacttgcagg
     1201 ggcgctcgga aacccgttgg cttgcaatga ggtcgagttg atctcgggcg cgtagagttc
     1261 gtccagaccc aggcccgacg gcccattgag gatctcgtag gcggggtgct tgtcggacca
     1321 cgcggtgcgc aggccagcct gatggatgat ctcaaagatc gtgttcgtct tgttcttcat
     1381 gtagagatga ggccacacct tctcgcaacc gctcgcggtc ttgcgcagcg ccaggtgggc
     1441 gggattgatg tgcgggttgc gagggtcgaa ctccgcgccc gggggcggcg atccgccatc
     1501 cagcttggtc aggtcccagt cgagggcctc gaagttgctc acttcggttc cgggcgtacc
     1561 cgtgcagttg ctgcccggtg ggtacagggt ggcgtcgtaa gtatcgtcat agaagacacc
     1621 gtggctaatc gggctaccgc cggttgcaaa ggccaggagc ccaggaaacg agtccgaagg
     1681 cttgg
//
LOCUS       DATAQL010001171         7600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5003854, whole genome shotgun sequence.
ACCESSION   DATAQL010001171 DATAQL010000000
VERSION     DATAQL010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5003854"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..889
                     /gene="msrB"
                     /locus_tag="VHS97_20175"
     CDS             320..889
                     /gene="msrB"
                     /locus_tag="VHS97_20175"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX3450583.1"
                     /translation="MDSSKRLMAAGVVVVVISAVAGGVVVTSQATNSTNEPNRTTISM
                     PTKPNDSGSPTDEKEKEWKSRLTVQQYAVTRKKATEPPFSGKYWDNKAQGIYKCVCCA
                     TPLFESSTKFDSGTGWPSFYQPIDDQKIDTEVDFSLFTSRTEVLCHSCKAHLGHRFDD
                     GPEPTGLRYCINSAALDFQPAPSSPKNGG"
     gene            1111..1710
                     /locus_tag="VHS97_20180"
     CDS             1111..1710
                     /locus_tag="VHS97_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3450584.1"
                     /translation="MTLTPAAQKFVLHWGEMGQAWGINRTMAQVHALLFVAPSPLDAE
                     EISSLLDVSRSNVSTSLRELITWGVVRRVHIIGDRRDRFEALKDVMETFRVIMAERKR
                     REMDPTIALLEHCVEEAKVGGPSEEYTRDQLEKMLEFTRMVTTWYGHIDNLSTPSLLR
                     LFRGGSLLAKVFGRGKLSKSKITSDHEKAEAGAGSISRS"
     gene            complement(1821..3470)
                     /locus_tag="VHS97_20185"
     CDS             complement(1821..3470)
                     /locus_tag="VHS97_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450585.1"
                     /translation="MGRQRSLEHALDRRRYRALILGPCACILLVAALPGQAREDSPAV
                     PHASAAEPIERRPYRISLHMAIDPSARIDLLKRADLIREWQILVRRFIGAPWIVSIAD
                     PPYPLANLELDSLGPDAFASVGSFDKVWVVRVTRSDRDSVLVFTGREYDTSTRQLGPL
                     QVRRVSALSDAPRAMLQFARDLFNPTAAITGQEGGRAILTVQGASIAPASPIGAVVAK
                     GMVFQPLRLVSRNDGKVQVLKIIWTYLQAESVDGAVARCAIISGLRDPLSKRYSRPNS
                     LVALGLKSGNSPLKLRFVSGPDQTPAAGYTLTARLAPDGQSRELGTTDRAGRIVLKPG
                     FARSLVIMRLLAGSVEPIAEFPMMPGESGEERVISVDPKPHTVALESQLDSLRDEVVD
                     LVALRARLEARMKARLEGEDWTGLEEALKEFARLTPRDKFAQQLTQLKDDAAHRQAEL
                     KTAVLTKTAQAQITELQSMIDRYMEDDAFRAYSDAVSKFRSETAAKAKAVAKKAATAA
                     LVRPSEAIKKEQPAPASPSVPAPGAPKTKQAPPLKPSETPF"
     gene            complement(3437..5986)
                     /locus_tag="VHS97_20190"
     CDS             complement(3437..5986)
                     /locus_tag="VHS97_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3450586.1"
                     /translation="MNNHRETGESVNVGVRCRLAIAVSTAVVLIGMLGSMPFCCAQDA
                     GTLPVPGTSDLLRAEPFDRLTLIDNSVFLVEPVSPRPLPPIEGEGKRKGSNKKSAIPP
                     EGNIIVGVPTKLELPGAQTDADGVTSEEVRLHLFQAGPNEVRDFKVKRTNIKKIEYFE
                     DLLLQECARQVTAHDYARAFECCLRVQARNPGWPKLDEHVNEVLFSEGSRTLLDGDSE
                     RGLRLLRELLGRKRDYPGLLDQIAGAYSKRIERALKIGLYARGRRVLHELEEVVGEHS
                     LVKQMRALYVARAAGLVKDAEAMAMPERLDVLTEALRIWPELDGALALYEKAFAVAPT
                     VDVAVIDVATPVGPWIRSPADARVSRLLYRPILDRDDEEALGGRKPGQLASAIETSEL
                     GRRLLIRIRSGVVWSDLSRPVSAIDVARALVDRTDPHSPGFEARWAELLDRVEMTDEI
                     RVEVRLHRAPLRVGAWLLGPIGPAHASVDGRVATSSEDRPLVTDGDFWCPVANADYGE
                     LRLQVDQKTRAGDSASTPGPEPKNGPFRVKRIREKRLSLGQTAAGVLRRGDVTLVEHV
                     PSDQVAGLADSPEIKVGTYAQPIIHLIAIDGRNPALRNRALRRALSYAADRKVLLEDH
                     LLKHSPTGKDTVADGPFAKGSYADAPGVKPLESHPWLAKMLVAAARKELGGQPIRLKF
                     EYPAIAEVKVIVEKLAESFRVAGLEITTTEVAPSRLESELRAGRRFDLVYRILRCDEP
                     VLGAGVLLCPGYDAPAETNALASGASPRILQLLIQLERAGEWVSARALAIQIDRESRD
                     ELPVIPLWQLADHYAWRDRLKGPAESASELYQGIETWEIAPWVARDPWNTH"
     gene            complement(5983..>7600)
                     /locus_tag="VHS97_20195"
     CDS             complement(5983..>7600)
                     /locus_tag="VHS97_20195"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3450587.1"
                     /translation="CIAVEYLGHVDGSIPCAITRLGRYLVIPENNSLTDSRWHVLVVD
                     ESGAKDRPVQELEVSGWTWSTPASAGSIVWAAGDKGGYEAFAVGDYGTPTPFRSVARV
                     TADAVASGPAFALARSDRELWVASGHTGRFELDAERGKIELKASLAQPGAALAPIQSK
                     GRHVVMTFHDDDTGGVALWGIDSETGAIAWKTIVAAPWPTAPLGLTGSDDLAMICRDG
                     REIRIAAQQIGRGGFVIEAIPQPGEFTLPAGRRVRVEADGKAFAAIAPKDRPDFVWVQ
                     DPDKPGAWRKVGLPTPPAADPIGWGDGILIPGADARAYLIDPLTSRSRAEPFVPKFDR
                     DRQGTWLCPTRLDQETVVLAEDVGRILRVAKKTMPVVRLVGEAQAVLEERIIADPAST
                     GSAVIVVTADRRVRALAARDLSPVGSWALEAPLAGPPIGIDDMCFVTDRAGGVMAFGR
                     DGQRIWSIQLESAVVGAPLIRDQSVWLITQRGKLHVRALSDGQARGELALSVLPTGGL
                     IMAGKQLLVPVGQGTVRTLTAIPGVEKKQP"
BASE COUNT         1487 a         2513 c         2200 g         1400 t
ORIGIN      
        1 gaagtcatgt ccccaggttg attcgcattg aggatctgaa gtaagaggac gggtctcctt
       61 tcccgcacga tcggtcttct gcgccggctc gattagctct gatcacggca agcgtctcct
      121 cgatgcgttc gactgcattc aatcgtcgtt cctcgattga atctgtctgc tggttcacgt
      181 ccggtagagg tcacctggat ttcggcctct gtaaggaacg tctcgttcac gcggttgttc
      241 tgtccaaccc gtggcacaaa gagtcaaaat cgaacttttt gtttccagga tttggaaatc
      301 atccgcggga ggtacgatca tggattcaag caaacgtctt atggcggccg gagtcgttgt
      361 cgtggtcatt tcggccgtgg cgggcggcgt cgttgtaaca tcgcaagcaa ccaattcgac
      421 gaatgagccc aaccggacta cgatttctat gccgaccaaa ccgaatgaca gcggatcgcc
      481 gaccgatgaa aaagaaaagg aatggaagag ccggctaaca gtacagcagt atgccgtcac
      541 acgcaagaaa gcgaccgaac cgcccttcag tggcaagtac tgggacaaca aggcccaggg
      601 catttataag tgcgtttgtt gcgcgacacc gctttttgaa tcaagcacga agtttgactc
      661 gggcaccggt tggcccagct tttaccagcc gatcgacgat cagaagattg acaccgaggt
      721 ggactttagc ttattcacct ctcgtacaga ggttctctgc cacagctgca aagctcacct
      781 cggtcaccga ttcgacgacg gccccgagcc taccggtctc cgctattgca tcaactcggc
      841 agcgctcgac ttccagcctg ctccgtcctc gccgaaaaac ggtggctaga tgaggggtag
      901 tcaaaacctg atacatagcc tccaatcgct ggcaaatgtc tcccaagctt ttggccatac
      961 gcccgtctgt caatgcttgc ggcacaagat ttcgcttctg atctagagca tcgactcgcc
     1021 ccacaacgag tcggcggcac tcttgtcgtt gagtccgcgc gcaagtatta tttcagtaga
     1081 aacggtttga ccagacaggg ccgaagcaac gtgacactca cacccgccgc gcagaagttc
     1141 gtgcttcact ggggcgagat gggccaagcg tggggcatca accgcacgat ggcccaggta
     1201 catgcgctcc tgtttgtcgc cccgtcgcca ctcgacgccg aagaaatcag ctcgctgctc
     1261 gacgtgagcc gatccaacgt ttcgaccagc ctgcgcgagt tgatcacgtg gggcgtcgtt
     1321 cggcgcgtcc acatcatcgg cgaccgacgc gatcggttcg aggcgttgaa agacgtcatg
     1381 gagacctttc gtgtcatcat ggccgaacgc aagcgccgcg agatggaccc caccatcgcc
     1441 cttctggaac attgcgtcga agaggcaaaa gtgggcggac cgagcgagga atacacccgt
     1501 gaccaactgg aaaagatgct cgagttcacc aggatggtca caacctggta cggccatatc
     1561 gacaaccttt cgaccccatc ccttctacgc cttttccgcg gtggatcgct cctggcgaag
     1621 gtcttcggca gaggcaagct ttccaaatcg aaaatcacca gcgaccacga aaaagctgag
     1681 gccggggccg gctcgatctc tcgatcgtga ctgattgatg ccaagtcatt ttggcctcac
     1741 tatgaatctg agccggcgac cgtgggccga tgcgcacgta gcgctctatt gcggctgaga
     1801 tcgctttcgc gcacacctct tcagaatggc gtctccgaag gctttagcgg cggcgcctgc
     1861 ttggttttcg gggcaccggg cgcggggacc gagggcgagg caggcgccgg ctgttccttt
     1921 tttatcgcct cgctcggtcg aaccaaggcc gcggtcgctg ccttctttgc cacggccttc
     1981 gccttggcgg ccgtctcgga tcggaacttg gagacggcgt cagaatacgc tcgaaatgcg
     2041 tcatcctcca tatagcgatc gatcatggat tggagctcgg ttatttgtgc ctgggccgtc
     2101 ttggtcagca cggccgtctt gagttccgct tgccgatggg cggcgtcgtc cttgagttgc
     2161 gtgagttgct gggcaaactt gtcgcgtggg gtcagtcgcg caaattcctt gagggcttcc
     2221 tcgagcccgg tccaatcttc tccttccagt cgcgccttca tccgcgcctc cagccgcgcc
     2281 cgcagcgcga ccagatcgac gacttcatcc ctcagggaat cgagctgaga ttctagcgcg
     2341 acggtatgtg gcttgggatc gactgaaatc acccgttcct ccccgctttc gcccggcatc
     2401 atcgggaact cggcaatcgg ctccacgctg ccggcaagaa gtcgcatgat gacgaggctc
     2461 ctggcaaaac caggcttgag cacgatccgt cccgcacggt cagtcgtacc gagctcacga
     2521 gactggccgt ccggtgccaa ccgcgccgtc agcgtatagc cggcggccgg agtctggtcg
     2581 ggcccggaca caaatcgcag cttgagcgga ctgtttcccg acttcagccc caaggccacc
     2641 aagctgttgg gacgcgaata tcgcttggat aacggatcgc ggaggccact gatgatcgcg
     2701 cagcgagcga ccgctccgtc caccgactct gcctgtaaat atgtccaaat aatcttcaag
     2761 acctggacct tgccgtcatt gcgggatacc aaccgcaacg gctgaaaaac cattcccttg
     2821 gcgacgaccg ccccaatcgg gctcgcgggc gcgatcgagg ccccctggac ggtcaaaatc
     2881 gcgcggcccc cctcctgccc cgtgatcgcc gcggtcgggt tgaagagatc acgcgcgaac
     2941 tgaagcatcg cgcgaggcgc gtccgacaga gcagacactc ttcgtacctg cagcggaccg
     3001 agctgccgag tcgacgtatc gtactctcgg cccgtgaaaa ccagcaccga atctcggtcg
     3061 gaacgagtga cccgcaccac ccacactttg tcaaaggagc cgacgcttgc aaaagcgtcg
     3121 ggaccgagag aatcaagctc gagattggcc agcgggtagg gcggatccgc gatcgaaacg
     3181 atccacggtg cgccgatgaa gcgccgcacc agaatctgcc actcccgtat caagtccgcc
     3241 cgtttcagta ggtcgatccg cgcggacggg tcaatcgcca tgtgcagcga gatccgatat
     3301 ggccggcgct cgatcggctc ggccgccgac gcatggggca cggccggcga atcctcccgt
     3361 gcctgtcctg gcagcgcggc cacgagcaag atgcacgcgc atgggcccaa gatcaacgcc
     3421 cgataacggc ggcgatctag tgcgtgttcc agggatctct ggcgacccat ggcgcaatct
     3481 cccatgtttc gataccttga taaagctcag aggccgattc ggcaggtccc ttcaaacggt
     3541 cgcgccacgc gtagtggtcg gctagctgcc agagcgggat caccggcagc tcatcgcgcg
     3601 attcgcgatc aatctggatg gccaatgccc gcgcgctcac ccactcgccg gcccgctcga
     3661 gctggatcaa tagctgcaag atcctggggc tggctcccga cgccagagcg ttcgtttcgg
     3721 caggagcgtc gtagcccggg cagagcagca ctccggcgcc caagaccggt tcatcgcagc
     3781 gcaggatgcg atagacgaga tcgaaacggc gccccgcgcg cagctcggat tccagtcgcg
     3841 aaggcgcgac ttccgtcgtc gtgatctcca agcccgcaac ccgaaatgac tcggcgagct
     3901 tttcaacgat caccttcacc tcggcaatcg cagggtactc aaacttgagc ctgatcggct
     3961 gtccccccag ttccttgcgg gcggcggcta ccagcatttt cgccagccac gggtgcgact
     4021 cgagcggctt gacacccggg gcgtcggcat agctcccctt ggcgaaaggt ccatcggcaa
     4081 ccgtgtcctt cccggttggg gagtgcttca gcaggtgatc ctcgagcaag accttgcgat
     4141 ccgcggcata ggaaagagcg cggcgcaatg ctcgatttcg cagagccggg ttgcgcccgt
     4201 cgatcgcaat caagtggatg atcggctgcg cgtacgtgcc aaccttgatc tccggcgagt
     4261 cggcaagccc cgcgacctgg tcggacggaa cgtgctcgac caaagtcaca tcccctcgac
     4321 gcaacacccc ggccgccgtc tgtccgagcg agagccgctt ttcgcgtatg cgcttgaccc
     4381 gaaaaggccc attcttgggc tcagggcctg gagtgctggc cgaatcgcct gcgcgcgttt
     4441 tctggtcgac ttgcagccgt aattcgccat aatcggcatt ggcgacaggg caccagaagt
     4501 caccgtcagt gacgagcggc cgatcctcgc tcgatgtcgc cacgcggccg tcgacactgg
     4561 catgagccgg cccgattggc cccaagagcc acgcgcccac ccggagcgga gcccggtgaa
     4621 gccggacctc gacccgtatc tcgtcggtca tctcgacccg gtcgagcagc tcggcccatc
     4681 gcgcctcgaa accgggggaa tgaggatcgg tgcggtcgac caaggcgcgc gcaacgtcga
     4741 tcgccgacac cggccgggat agatcggacc atacaacgcc cgaccggatc cgaatcaaaa
     4801 gccgccgtcc cagttcggac gtctcgatgg ccgaggcaag ctgccccggc ttccgccccc
     4861 caagggcttc ctcgtcgtcg cgatccagga tcggacgata cagcaatcgg ctgacgcggg
     4921 catccgccgg cgagcgaatc caagggccca ccggagttgc aacatcgatc acagcgacgt
     4981 cgaccgtcgg cgctaccgcg aatgccttct cataaagggc caaggcaccg tccagctcgg
     5041 gccagatccg taatgcttcg gtcagtacgt cgagccgctc gggcatcgcc atagcttcgg
     5101 catccttcac cagccccgcg gccctggcca catacagggc tcgcatttgc ttgacgagcg
     5161 agtgctcacc cacgacctct tccagctcat gcaaaacccg gcggccccgc gcataaaggc
     5221 cgatcttgag ggcacgctcg atccgcttgc tataagcccc ggcgatctga tcgagcaggc
     5281 cagggtaatc ccgtttgcgt cccaggagct cacgcaataa cctcaatccg cgctcggaat
     5341 cgccgtcgag caaggtccgg ctgccctcgg aaaacagcac ttcattcacg tgctcgtcaa
     5401 gcttgggcca tcccgggttc cgcgcctgca ctcgcaagca gcactcgaaa gccctggcgt
     5461 aatcgtgggc tgtcacctgc cgagcgcatt cctgcaagag aagatcctcg aaatattcga
     5521 tcttctttat gttcgtgcgc ttcaccttga aatcgcgcac ttcgttgggc ccagcctgaa
     5581 atagatgcaa cctcacttct tcactcgtaa cgccgtcggc gtcagtttgc gcgccgggca
     5641 gctcgagctt ggtcggcacg cccacaatga tgttgccttc gggagggatg gccgacttct
     5701 tgttactccc cttccgctta ccttcgccct cgatcggcgg gagcgggcgg ggacttaccg
     5761 gctcgaccag aaaaaccgaa ttatcgatca gcgtgagccg atcgaacggc tctgcccgca
     5821 gcagatcgct ggtgccaggc acgggaagcg tgccggcgtc ctgagcgcaa cagaacggca
     5881 tcgagcccaa catgccgatc agcaccaccg cggtcgacac ggcaattgcg agccggcatc
     5941 gtacccccac gttcaccgat tcgcccgttt cacgatggtt attcacggtt gcttcttctc
     6001 cacccctggg attgcggtca gcgtgcgaac ggtcccctgc ccgaccggaa ccagcaactg
     6061 tttgcccgcc atgatcagac cgccagtcgg caagaccgag agtgcgagct cgccgcgtgc
     6121 ctggccatct gacagtgccc ggacatgcag cttgccccgc tgcgtgatca accagaccga
     6181 ctgatcccgt atgagcggag ctccgacgac cgccgattcg agttgaatcg accagattcg
     6241 ctgcccatcc ctgccaaagg ccatcacgcc ccccgctcga tctgtgacga agcacatgtc
     6301 gtcaataccg atcggcggcc ccgccagagg tgcctcgagc gcccaggatc cgaccgggct
     6361 caagtcgcgc gcggcaagtg cccgcacgcg ccggtcagcc gtcaccacga tgacggcact
     6421 tcccgtcgat gccggatcgg cgatgatccg ctcttccagt acggcttggg cttcaccgac
     6481 caagcgaacg accggcatcg tcttcttcgc gacccgcaga atccgtccga catcctcggc
     6541 gagcaccacg gtttcttgat ccaacctagt gggacagagc caggtcccct gccgatcgcg
     6601 gtcgaacttg ggtacgaacg gttccgcgcg tgagcgggac gtcaacggat cgatcaaata
     6661 cgcccgtgca tccgcaccgg gaattaaaat cccgtctccc cacccgatcg ggtcggccgc
     6721 gggcggggtc ggcagaccga ccttgcgcca ggcaccgggc ttgtcgggat cttggaccca
     6781 gacaaaatct ggcctgtcct tgggcgcgat cgccgcgaaa gccttgccat cggcctctac
     6841 ccggacgcgc cggccggccg gaagggtaaa ttcgcccggc tgcggtattg cctcgatcac
     6901 aaaaccaccg cggccaatct gctgcgcggc gatccggatc tcgcggccgt cgcgacagat
     6961 catcgccagg tcgtccgagc cggtcaaacc aagcggtgcc gtcggccaag gggccgcgac
     7021 gatcgtcttc caagcgatcg cgccggtctc tgaatcgatt ccccagagcg cgacgccccc
     7081 cgtatcatcg tcatgaaacg tcatgacaac gtgccggccc ttgctttgaa tgggcgcgag
     7141 tgctgctccg ggctgcgcca gcgatgcctt cagctcgatc ttgccgcgct cggcatcgag
     7201 ctcgaaccgg cccgtatgac ccgacgcaac ccagagttcg cgatccgagc gcgccagcgc
     7261 gaaggccggc cccgacgcca cagcatccgc cgtcacccgg gccaccgacc gaaacggagt
     7321 cggggtcccg taatcaccga cggcaaacgc ctcgtagcct cccttgtcac cggcagccca
     7381 gacaatcgag cccgcgctcg cgggtgtgga ccacgtccag ccggaaacct cgagctcctg
     7441 caccggccgg tccttcgctc cggattcgtc gacgaccaag acgtgccacc ggctatccgt
     7501 cagtgagttg ttttcgggga tcaccaggta ccggcccagt cgagtgatgg cacacgggat
     7561 cgagccgtcc acgtggccga gatactcgac ggcgatacac
//
LOCUS       DATAQL010001172         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_118803, whole genome shotgun sequence.
ACCESSION   DATAQL010001172 DATAQL010000000
VERSION     DATAQL010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118803"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VHS97_20200"
     CDS             complement(<1..252)
                     /locus_tag="VHS97_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450588.1"
                     /translation="MDGRLDETSKHPLLRFLSYLKPNAQLVVGAALMGIGKFTLPLAF
                     PIAFRYVVDVLLAAHPHPDGISLKIDHWCIGIANLLGLGT"
     gene            552..1898
                     /locus_tag="VHS97_20205"
     CDS             552..1898
                     /locus_tag="VHS97_20205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3450589.1"
                     /translation="MATPPAGTDVSATGSVAVGSGESVRETFAIISAIVILLVYQGYT
                     LSIAGIASPWISKSFALNQSKLAELFAWMSLSAFGSLALARWADRVGRRRIILLGLLL
                     SPLLSLASAAAGGPVSFAVFQILIAALLGGSVTSAIALLAEELPVNQRARGQAAAATA
                     SAVGGVLGYFVIPVLLKLGYSWRWLFAPSVAGIALLLPVARMLPETQWAEQRSKGTTT
                     KSRIYDVFNLIYRRRALALISNAALATVAGTAVNGWLYYEAVSIIGLSPTKASTLVVT
                     GMLVGMLGFPIGAWTSERFGRVPTVTYFGGAAWLGAIAFYWWPNVPFAWPMVWLTSIY
                     CWFKVGSGIMTVGANAAATELFPAALRSTIMGWQMIVGALFSMLAQAMIAALIGPLGG
                     LTNVIGYLALLGLPSALIFGLFIDETRGLPLKVAAKEDEWEKVPGRAPLAASLHKP"
     gene            2093..>2369
                     /locus_tag="VHS97_20210"
     CDS             2093..>2369
                     /locus_tag="VHS97_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450590.1"
                     /translation="MDVPTGQSPAVLDSVEIYDVENGQFLPVEKMDSRRDHHDACRLT
                     DGSVLIVGGVDAVLVPTIMFPGPAMPWILKSAEVFSPEDGFGKAVYMG"
BASE COUNT          404 a          715 c          733 g          517 t
ORIGIN      
        1 cgtccccaac ccgagcaggt tggcaatgcc gatacaccag tgatcgattt tgaggctgat
       61 gccgtccgga tggggatgag ccgcaagcag gacatcgacc acgtaccgga aagcaatcgg
      121 gaaggcgagc ggaagggtaa atttgccgat gcccatcagg gcggcaccca ccactagctg
      181 cgcgttcggt ttcagatagg acaggaacct gagcaacgga tgcttcgagg tctcgtcaag
      241 tctgccgtcc atattcgtaa aacgcctttc gtttgtcggg agtctagccc cccgaagtct
      301 cagccggtaa actgcaatcg gcgtgccccc taggtgcggc cggaggatca ttcaatttct
      361 tctgactttg ggggtatttt gcgcaggaac tgcacttcga acgcagttgg gctaacgcat
      421 ttcgtgaggc tcgttagctc aactggtgta cacggtttcc ggtagcgagt gagggggggc
      481 gtggccgtgg cgcgacaacc agaacaaaac aagaatttgg ccgggccagg cgacgtttcg
      541 gccgtctagg gatggcgacc cctccagccg gaaccgatgt ctctgctacg ggcagcgtcg
      601 cggttggctc gggtgaatcg gttcgcgaaa ccttcgcgat catctcggcg atcgttatcc
      661 tgctcgtcta tcagggctat accctctcca tcgctgggat agcttcaccc tggatttcca
      721 agagcttcgc gctcaatcag tccaagctcg ccgagctctt tgcctggatg tcgttgtcgg
      781 cctttggttc gctggcactt gcgcgctggg ccgaccgggt cggtcgacgc cggattatct
      841 tgctcggctt gctgctctct cctctgctct cgctggccag cgcggcggcc ggcggtccgg
      901 tctccttcgc ggtttttcaa atactcatcg cggcgctttt gggcggctcg gtaacgtccg
      961 cgatagctct gctggcggag gagcttcccg tgaaccagcg cgcccgcggg caggccgcgg
     1021 ccgcgaccgc cagcgcagtc ggcggtgtgc tcggatactt cgtaattccg gtgctgctca
     1081 aactcggtta ttcatggcgc tggctgttcg ctccatccgt cgcgggcatc gcgttgcttt
     1141 tgccggtggc tcggatgctt ccggagacgc aatgggcgga gcagcgatcc aaaggcacca
     1201 ccacgaaaag ccgcatctac gacgtcttca acctgattta tcgtcgccgg gcactggcac
     1261 tgatctcgaa cgccgcgctc gccacggtgg cgggtaccgc agtaaatggg tggctctact
     1321 acgaggcagt ttccatcatc ggactatcgc cgaccaaggc aagtacactg gtagtcaccg
     1381 gaatgctggt cggtatgctt ggctttccga ttggcgcctg gacctcggaa cgcttcgggc
     1441 gggtgcccac cgtcacctac ttcggcggtg ccgcctggct gggtgcaatt gctttctact
     1501 ggtggccaaa cgtgccgttc gcctggccga tggtgtggct tacctcgatt tactgctggt
     1561 tcaaagtcgg ctccggcatc atgacggtgg gggcaaacgc ggcggcgaca gagcttttcc
     1621 ccgccgcgct acgctcgacc atcatgggct ggcagatgat cgtcggggca cttttttcga
     1681 tgctcgcgca ggcaatgatc gctgctttga ttggtccgct cggcggcctt accaacgtga
     1741 ttggctactt agcgctgctt ggcctgccca gtgcgctcat cttcggactg ttcatcgatg
     1801 aaactcgtgg cttgccactc aaggtcgccg ccaaggaaga cgagtgggag aaagttcccg
     1861 gtcgggcgcc cctggcggct tcgctgcaca aaccatgacg acgcgcttga gcagaaacgc
     1921 acgggtgctt gccgcgctca gcgtggcatt gctgatggtc agtttgtgcg tcacaggtgc
     1981 gcccgcccgc acccgtcatc gacgccgcaa accggcgccc accttgacgc ctaccgcaac
     2041 cccgactgtc gtactacgcc cggtggtgct tatcaccgga ggtaccggta ccgtggacgt
     2101 gccgaccggc cagagccccg cggttctcga tagcgtcgaa atttatgatg tcgagaacgg
     2161 tcaatttctg ccggtcgaga aaatggactc gcgccgcgac caccacgatg cgtgcaggct
     2221 tacggacggc agcgtgctca ttgtcggggg agtcgatgcc gttctggtcc ccacgatcat
     2281 gtttcctggg ccggcaatgc cctggattct gaagtcggcc gaagttttct cgcccgagga
     2341 tggctttggc aaggccgtat acatgggcg
//
LOCUS       DATAQL010001173         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1339834, whole genome shotgun sequence.
ACCESSION   DATAQL010001173 DATAQL010000000
VERSION     DATAQL010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339834"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VHS97_20215"
     CDS             <1..658
                     /locus_tag="VHS97_20215"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX3450591.1"
                     /translation="RARRRREYVGPWVPEPVDTRGRESALSESLSQGFLLMLERLSPD
                     ERAAFLLRTVFDYEYAEIGEVVGKAEATVRQLVSRARRRLGLNGERRFRASPARADVL
                     VERFVAACRAGDVKAVEQMFTEDAEVHSDGGGRVSAARVVVRGRERAARFLAGVFSKK
                     RRDCEMHATTVNGEPGVVFISYGTVIQVVSLSLGDGVQAVYMTVNPDKLARWSVAEVD
                     "
     gene            700..1455
                     /locus_tag="VHS97_20220"
     CDS             700..1455
                     /locus_tag="VHS97_20220"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3450592.1"
                     /translation="MKIVVIGGSGLIGTKLVNDLRKRGHEVVAASPSTGVNTVTGEGL
                     TEALIGTQVVVDVANAPSWEDKAVLAFFETSGRNLLAAEAVAGVGHHIALSVVGTDRL
                     LASGYFRAKMAQENLIKASKIPYTIVRATQFFEFVGAIAQLASDGQTVRLPPALMQPI
                     VSDDVAAALADVAVADPLKRTVDLAGPEKIRMDDLVRQFLVASGDTREVITDPQARYY
                     GIAVNDQSLTPGDNPLIGSTRFEDWLSRSRTQG"
BASE COUNT          307 a          483 c          617 g          332 t
ORIGIN      
        1 gcgggctcgc cgccggcgag agtacgtggg gccgtgggtg ccggagccgg tggatacgcg
       61 gggccgggag tctgccctca gcgagtcgct ctctcagggg ttcctgctta tgctggagcg
      121 gttgagcccg gacgagcggg cggcattcct gctgcgcacc gtgttcgact acgagtacgc
      181 agagataggg gaggtcgtcg ggaaggccga ggccaccgtc cggcaactcg tcagccgcgc
      241 ccgtcgtcgg ctcgggctga acggggagcg gcggttccgg gcgtcgcctg cgcgggcgga
      301 cgttctggta gagcggttcg ttgccgcctg ccgggcgggg gacgtaaagg ccgtcgagca
      361 gatgttcacg gaggacgcgg aggttcactc cgatggcggg ggtcgggtgt cggcggcgcg
      421 ggtcgtggtc cgcgggaggg agcgggccgc ccggttcctg gccggggtct tcagcaagaa
      481 gcgtcgggac tgcgaaatgc acgcgacgac ggtcaatggc gagccggggg tggtgttcat
      541 ctcgtacggg acggtgatcc aggtcgtgtc gttgagcctc ggcgatggcg tgcaggccgt
      601 atacatgacg gtaaacccgg acaagctggc gcggtggtcg gttgcggaag tcgattagga
      661 cggccacccg gtgtagtttc acaagcgagg agatttgcca tgaagatcgt ggttatcggt
      721 ggtagtgggt tgatcgggac gaagctcgtg aatgaccttc gcaagcgtgg ccatgaggtc
      781 gtggcggcgt cgccatctac aggcgtcaac accgtgaccg gtgagggact gactgaggca
      841 ctgataggta ctcaggtcgt cgtcgacgtg gcgaacgcgc cttcgtggga ggacaaggca
      901 gttttggcgt tcttcgaaac gtcagggcgc aacctccttg ccgcggaagc ggtcgctggc
      961 gttggacatc acatagcgct ttcggttgtc gggaccgacc gcctccttgc aagcggttat
     1021 ttccgcgcga agatggccca ggaaaacttg atcaaggcct ccaagatccc ctatacgatc
     1081 gtccgggcaa cgcagttctt tgagttcgtc ggagccattg cccaattggc cagcgacggc
     1141 caaacggttc ggttgccgcc tgctctgatg caacccatcg tgtccgatga tgtcgctgcg
     1201 gcgctagccg atgtcgcagt cgcggatcca ctgaaacgca cggtcgatct cgcgggccca
     1261 gaaaaaatcc gtatggacga tctcgtgcga caattcttgg tcgctagcgg agacacgcgc
     1321 gaagtgatca cagatccaca agcccggtat tacggcatag cggtgaacga tcaaagcctc
     1381 accccgggcg ataacccgct catcggctca acgcggttcg aggactggct cagccgctcc
     1441 agaacccagg gatgagccag cggtctcgac gaagatccgc gcgtgatata atcacgcact
     1501 tggtccacgc ggcttcgagc gaggttgggt cgggcgactt gtcggcagtc gagatggccg
     1561 aaaggtaacg cgatgacaca gagtgacctg gtgcctgccg acgagtcttt ccagagactt
     1621 gcagctcgtc ggcgtgttcg cagagctgcc atgctgattg gggcgatcgg tctcgccgtc
     1681 accggctgtg tactcgccta tcattcgcta tggagtttca gggacgagtg ggcttcatt
//
LOCUS       DATAQL010001174         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2104426, whole genome shotgun sequence.
ACCESSION   DATAQL010001174 DATAQL010000000
VERSION     DATAQL010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104426"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VHS97_20225"
     CDS             <1..374
                     /locus_tag="VHS97_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450593.1"
                     /translation="DECQRLTQTLRSENARLKDRVLALQGQNRDYAERAVDDARRLAT
                     ADDAIEHLEHSVQAYQDERARLEAAYNKLASNLGAPELSKDELQRRADQTTSQMTRSA
                     APSPSRETKASASTHTDEAPR"
     gene            493..1197
                     /locus_tag="VHS97_20230"
     CDS             493..1197
                     /locus_tag="VHS97_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX3450594.1"
                     /translation="MRIICSALCVGFVTLSASVASAAGIQGDYVEARTADVFTGPCFS
                     NAEVFIYGNHAVLAWKVNEGAWKGVDLRGLCVAAAVDGTTTFSEDQPDKATAVVIVDS
                     KASGPQREALIDMAKALGGARLNRITAVKNARMSLKLEDHAMSASESSHEAHGMPQSP
                     RASFWAAGLARIVTRPLDERDHACGNEVVAYPPLSSSVSAQPAFTLGHVFQGEGLGSR
                     WDDPNCRSSFVGRFAL"
     gene            1325..2011
                     /locus_tag="VHS97_20235"
     CDS             1325..2011
                     /locus_tag="VHS97_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450595.1"
                     /translation="MRVFLVSAFLVSTATAGFAQESITIAALKEPPPQSISAEIRNAL
                     IADGYRIQDKDGHTIADVWLRKGVPGSGKPEGPKGAIQFPFLADGELVGALQFATEGH
                     DYRDQPIAKGVYTMRYGLQPVNGDHLGVSTYRDYSLLLPASKDQSLALIGRKQLDERS
                     AESAGTSHPAIFLLLEAPPESSKSAGSVIHDAEKNTWSVVVPLSLQVKGQAEPLAFPV
                     RLVVIGAAAA"
     gene            2134..3606
                     /locus_tag="VHS97_20240"
     CDS             2134..3606
                     /locus_tag="VHS97_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX3450596.1"
                     /translation="MMPVSLLIALLLAFGIEPAQPGVPQSDVFARVLETFGGVTLVAA
                     LAFGLGLWVSFQVNHSGYATSRLRRRYALGVRVLTLLSLIVFGWIIHSVGWAKLVRTN
                     WGLGGSILVDDIVVFLPYLCIQLLVWWGLFYAERALQIRHDSNSESRLGLHLILKARQ
                     ALLILPVVLLYVIRRDVLARIFPGWEDNALAEPLQIAVLGTLVLAISPFFIRLAWPTH
                     SLPQGPLRRRLERIADRVGFRYTDVLVWDTGNMMVNACVTGILPGYRYVLLTDALIDS
                     LTPHESAAVFGHEIGHIAHRHLLYFALFFMGSLGVLSIFGEMVSRCGPWFAQLAWLTP
                     WNPSIFSEVVQSVLLLAFLGSYFWFVFGQISRRFERQADVFGSKVVSCDLANCPPHTD
                     LDHDLSAPPISGKQPTLCPVGIRIFADALANVARCNGLDPDGRSWRHGSIARRIAFLE
                     ELEFHPERERPFQRGVMQLRIGLGIVLIAAIVISVFTQLG"
BASE COUNT          668 a         1093 c         1105 g          843 t
ORIGIN      
        1 tggacgagtg ccagcggctg acgcagacac tgcgctcgga aaacgcccga ctgaaagacc
       61 gagttttggc gttgcaaggc cagaatcgag actatgccga gcgcgccgtc gacgatgcgc
      121 gtcggctagc gaccgcggac gacgcgatcg agcacctcga acatagcgtc caagcctacc
      181 aggacgaacg cgctcgtctc gaggccgcat acaataagct cgcgtcaaat ctcggtgctc
      241 ctgaactgtc gaaggacgaa ctacaacgtc gcgccgacca gacgacctcc caaatgaccc
      301 gatcggccgc accgtcacca tcgcgcgaaa ccaaagcctc agcatccacg cacactgacg
      361 aggcaccgcg atgacacgcg tgtccctctg ttcgccccgt ggcctcttga ctgtgggtgg
      421 aattgtctcc cataatcctg agtgattaac tactccggac gcttgggcca attcgattaa
      481 ggacaaagct ccatgcgtat aatctgttca gcattgtgtg tcgggtttgt gacgctaagc
      541 gcgagcgtcg ccagtgcggc gggaattcaa ggcgactatg tcgaggcacg taccgccgat
      601 gtctttacgg gtccttgctt ttccaacgcc gaagtgttca tttacggcaa tcacgcggtg
      661 ttggcctgga aggtgaacga aggggcatgg aagggagtcg acctccgcgg gctctgtgtc
      721 gcagccgccg tggatggaac aacgaccttc tcggaggatc aaccggacaa agccaccgcc
      781 gtagtgatcg tggatagcaa ggcgagcgga ccccagcgtg aggccttgat cgacatggcc
      841 aaggccttgg gcggggctag actcaatcgt atcactgccg tgaaaaacgc caggatgagc
      901 ctcaagctcg aagaccacgc gatgtcggca agcgagagct cacatgaagc tcacggtatg
      961 ccgcagtctc cgcgtgcttc attctgggcc gcggggctcg cccgaatcgt gacccgtccg
     1021 ctcgatgaac gggaccacgc ctgcggcaac gaggttgttg cgtatccccc gctctcctct
     1081 tcagtgagtg cgcagccggc ctttacgctg ggtcacgtat ttcagggtga aggtctcggc
     1141 agccggtggg acgaccccaa ctgccgcagc agcttcgtgg gccgtttcgc cctctgagtt
     1201 cgctttttcc tcgttttgga ccacccccca gtctgcccag ggaccgcgaa cgcggggccg
     1261 atttcttcga ggcattcctt ccaaacctgg gcagacttcg attcacaatc aaggccatat
     1321 ctcgatgcga gtgtttctgg tctccgcttt cttggtcagt acggccacag cgggctttgc
     1381 gcaagaatcc atcactattg cagcgctcaa ggagccccct ccccaatcga tttccgcgga
     1441 aattcgcaac gctttgatcg ccgatggtta tcgaattcag gacaaagacg gccatacgat
     1501 cgcggacgtc tggttgcgaa agggtgttcc gggctcaggg aagcctgaag ggcccaaagg
     1561 tgccatccag ttcccattcc tggcagacgg tgagctcgtg ggcgcgctcc agttcgcgac
     1621 cgaggggcac gactaccgcg atcagccgat tgccaagggc gtgtacacta tgcgatacgg
     1681 tctccagccg gtgaatggcg atcatctcgg tgtgagtacg tatcgcgact actcgctgct
     1741 cctgccggca tcgaaggacc aatcgctcgc tcttattggt cgtaagcagc tcgacgagcg
     1801 aagcgctgaa tcggccggca ccagccatcc ggccatcttc ttgttgctcg aggcaccccc
     1861 tgaatcttcc aagtcggccg gctcggtaat tcacgatgcc gaaaaaaaca cctggagcgt
     1921 ggtggtgccc ctgtctcttc aggtcaaggg ccaggcggag ccgcttgcgt ttcctgttcg
     1981 cctcgtggtt attggagcgg cagcagcatg acgcgcgcag ctcgcgactc cggagctcga
     2041 aattgggcca ggatggcgcg attcgattac cgtttgggtc cacgttgaga tccctcgaca
     2101 ccctggagag atgtctgtcg gcataaacca acgatgatgc ccgtttcctt gctgattgcg
     2161 ctcttgctcg ccttcggaat cgaaccggca cagcccggtg ttccccagtc ggacgttttc
     2221 gcgcgcgtgc tggagacctt cggcggagtc acgttggtgg cagcgctcgc gttcggactg
     2281 gggttgtggg tttctttcca agttaatcat tccggttacg cgacttcgcg gctccgacgg
     2341 cgatacgcct tgggcgtccg ggtcctcaca ttactttcgc tgattgtttt cggttggatt
     2401 attcactcgg tcgggtgggc caagttggtg cgcaccaact gggggctggg tggatcgatt
     2461 ctggtcgacg atatcgtcgt tttcctccct tatctttgca ttcagttgct cgtctggtgg
     2521 gggttgttct atgcagaacg ggcactccag atccgccacg actcgaattc ggagagccgg
     2581 ctcggattac acttgatctt gaaggcgcgg caggcgttgc tgatcctgcc ggtcgtcttg
     2641 ctgtatgtga tccgccggga tgtgctcgct cgaatttttc ccggctggga agacaacgct
     2701 cttgccgaac cgctgcagat cgccgtactg ggcactttgg tgctggcgat ctcgccgttc
     2761 ttcatccgat tggcctggcc gactcactcg ttgccccagg gaccgctgcg ccgccggctc
     2821 gagcggatcg cggaccgcgt cgggttccga tataccgatg tgttggtatg ggacacgggc
     2881 aacatgatgg tgaacgcctg cgtgacgggg atcttgcccg gatatcgcta cgttttgttg
     2941 accgacgctt tgatcgactc attgacaccg cacgaatcgg cggcggtgtt tggacatgag
     3001 attggccata ttgcgcaccg ccatctcctc tatttcgccc ttttcttcat gggaagcttg
     3061 ggcgtgctct cgattttcgg tgagatggtg tcacgatgcg gtccctggtt cgctcaactg
     3121 gcctggctga ctccgtggaa tccgtcgatc tttagcgaag ttgttcagag cgtgttgctc
     3181 ctggcattct tgggttccta tttttggttt gtttttggtc agatttctcg gcgattcgag
     3241 cgccaggccg atgttttcgg cagcaaggtg gtttcttgtg atctggcgaa ttgcccacca
     3301 cataccgacc tggaccatga cctttccgca ccgcccataa gcggcaagca gccgacgctc
     3361 tgtcctgtgg gaatccgcat ttttgcggac gccctcgcga atgtcgcccg ttgtaacggt
     3421 ctagaccccg atggtcggtc gtggcgacat ggcagcatcg cccgccgcat cgcattcctg
     3481 gaggaactcg agttccatcc ggagcgcgaa cgcccgtttc agcgtggggt aatgcaactc
     3541 cggatcgggc tgggcatcgt gctgatcgcc gcaattgtga tttccgtatt cacgcagctc
     3601 gggtagcgga cgtacacggt ctgtccgtta agggtttgcc gggctcgatc aactcgggtg
     3661 ttatctccta agtgcacccg ccgaagtcgt cgcgcagcgt aggtcgggg
//
LOCUS       DATAQL010001175         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_118929, whole genome shotgun sequence.
ACCESSION   DATAQL010001175 DATAQL010000000
VERSION     DATAQL010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118929"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..808)
                     /locus_tag="VHS97_20245"
     CDS             complement(203..808)
                     /locus_tag="VHS97_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX3450597.1"
                     /translation="MRKFALSLAVLALVAAPAFAGKYNKAVSVGDKAPDISGIPAVEN
                     GVDKSLTLSDIKEDVVVVVFLANHCPVVVGYEDRLIDFTKDYKEKGVKVVGVAVSTME
                     GDKIPGIKAYMSEKKSNYIYGYDESQAIGKAYGASNTPQFFVLDKERKIRYTGAMDDS
                     PPREDKVTKTYLRDALDAVLKGETVAVDETRPQGCGVSYKR"
     gene            complement(1270..1980)
                     /locus_tag="VHS97_20250"
     CDS             complement(1270..1980)
                     /locus_tag="VHS97_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450598.1"
                     /translation="MTPRSIFMLRFAAVIFLATASLIAVTGCDPRALAYFLQPFDPTI
                     EPKGPLLTEKKVVVLCNVTSGALAEFPSLERDVPRELTAVLRKKVKKITVVEPDKVAT
                     WVEAHPHWTDPADAARDFNADIAIFLEVEQFQIQSPGDLNMVHGEAKVHIQVFEMQYP
                     KNSKDKPIKDQPKEAHNIYDEYAETTFPNRGPLPIDSGNSRSAFKNTFLKIVTKEISW
                     HFVEHAPDDSIQDSRIEK"
     gene            complement(2009..2509)
                     /locus_tag="VHS97_20255"
     CDS             complement(2009..2509)
                     /locus_tag="VHS97_20255"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX3450599.1"
                     /translation="MIPQQFDRQPLGWPIRFWLVVAAAGLAGLLILARTLEPDPRGFG
                     THTQLGLRPCAFLTATGRLCPSCGMTTAYAWLARGQIDRSWKANPAGCIYALFSVPLM
                     MWMMSSAIANQPVGFQNLAKPLAGLVIAAVFLGLASWLIRLIVSPAVLVGPGGRLWTL
                     AGLTGL"
     gene            complement(2711..3376)
                     /locus_tag="VHS97_20260"
     CDS             complement(2711..3376)
                     /locus_tag="VHS97_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3450600.1"
                     /translation="MKIQHPILVRAIGTTGAALVWHLGRTLKYHFRYKDPTVDPEVAR
                     KTGQRYIYAFFHEVTLFPAYYWNWPEMHILISEHRDGELITQVVKRLGFSVVRGSTTR
                     GGVRALREMSLRIDQGHLCVTPDGPRGPRRSVHQGVAYIASRTGLPIVGAGMAFKRPW
                     RAKSWDQFCVPRPFTPAACVVPAAVSVPHDADRDAIESCRMEVERRMQAAMLEAEAWV
                     NEL"
     gene            complement(3554..>4756)
                     /locus_tag="VHS97_20265"
     CDS             complement(3554..>4756)
                     /locus_tag="VHS97_20265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX3450601.1"
                     /translation="VFMLDEVDKLGADFRGDPSAALLEVLDPEQNSTFRDHYLDVDFD
                     LSKVMFIATANMLETIPSPLLDRLEVLQLPGYSEEEKTHIAQKYIIPKQLDLHGLAPA
                     NLTISDASIRKIIADYTREAGLRNLEREIASICRKMARRRAEGKRAPSVVQPKDIAEL
                     LGPSRYLLELADRTGIPGVSTGLAWTPTGGEILFIEASASPGKGGLTLTGLLGESMRE
                     SAQAALSYLRSHAKALSLDASRFGKTDLHIHVPAGAVPKDGPSAGVAIAAALISLFRD
                     KPIKANLAMTGEVTLTGRVLPVGGVREKILAARRAGIETVLLPRHNEKDLVDIPPEVK
                     ADLTLRLVDTLDDVVSRLFEARPRATRTHPRALDEPSPRLPAAKLANRKKDRPMLDPK
                     TPRRPGTL"
BASE COUNT          984 a         1397 c         1409 g          966 t
ORIGIN      
        1 tcgcattttt ggcctcgaaa agatcggcaa cgggtggtcg cggctggaat aattcgagac
       61 gagccaccaa cgcaagttgc caagagaaaa caagccgctc ccagccagga gcggcttgtt
      121 caaagctgac aaatctgctg ctcacccaca tcccatcgaa agaggtatcg cttacgagca
      181 ggtacctcta tgagttcgga aattacctct tgtagctgac gccgcagccc tgaggccggg
      241 tttcgtcgac cgccacggtc tctcccttga gcacggcatc aagcgcgtcg cggaggtagg
      301 tcttggttac cttatcttcg cgcggcggac tgtcgtccat ggcacccgtg taacgaatct
      361 tcctctcctt gtcgagcacg aagaattgag gggtgttaga ggcgccatag gccttgccaa
      421 tcgcctggct ctcgtcgtag ccatagatgt agttgctctt tttttcgctc atgtaggctt
      481 tgatgccagg aatcttgtcg ccttccatcg tcgaaaccgc aacaccgacg accttgacac
      541 ccttttcttt gtagtctttg gtgaagtcga tcaggcgatc ttcgtacccg accactactg
      601 ggcaatggtt cgccagaaag acgacgacaa ccacgtcttc cttgatgtcg gacagggtca
      661 ggctcttatc tacgccattt tcaacggctg ggatgcctga gatgtcaggg gccttgtcgc
      721 caacgctgac cgctttgttg tacttgcccg cgaaagcagg ggccgcgacc aaagcaagga
      781 ctgcgaggct cagagcaaac ttgcgcatgg atgtgttctc ctcaagggat aatcaatccg
      841 gatcgggagg tgcgacgtgc ctctcccatt caatccacct atcgatgctc ccgggaatca
      901 ggtccgtccc aagatgcaca tctgcccggt ctaggtcgaa actacgccga cgcctgattc
      961 tgaatttttt aaaaccgacc tgactcggtc acagctcgcg atgcccaatc gttgcacgca
     1021 tgaagccatt cgtgagtcga ttaagcgctc cgaatcaata ggtgatcgac tcggacttga
     1081 ttataagaag cgtcaaacgc gagtcaactg tccctcgcgg gatcggctat cgcgcacgat
     1141 tagacgagtg ccgcgcgcgg accgggccga gtttgacact cggcccggta caaaaagtcg
     1201 attggaagac tgcaaaagcg gttcggggca caccggcccg gcggctgacc catcagtccc
     1261 cgtcagggct catttctcga tgcgcgagtc ttgaatcgaa tcgtcgggag catgctcgac
     1321 gaagtgccag gaaatttcct tggtaacgat cttcaagaac gtattcttga aggccgaccg
     1381 gctgttgcca ctatcgatag gcaacggacc gcggttggga aaagtggttt ccgcatattc
     1441 gtcgtaaatg ttgtgggcct ctttgggctg gtccttgatc ggcttgtcct tgctgttctt
     1501 gggatactgc atctcgaaca cctggatatg gaccttcgct tcgccgtgga ccatattcag
     1561 atcgccggga gattggatct ggaactgttc gacctcgaga aagatcgcga tgtcggcatt
     1621 gaagtcgcgc gccgcgtcgg ccgggtcggt ccagtgagga tgggcttcca cccaggttgc
     1681 gaccttgtcc ggttcgacca cggtgatttt cttgactttc ttgcgcaaaa ccgcggtaag
     1741 ttcgcgggga acatcgcgtt ccagcgacgg aaattccgcg agcgcgcccg aagtcacatt
     1801 gcacagaacg acgactttct tctccgtaag gagcggcccc ttcggctcga tcgtgggatc
     1861 gaacggctgg aggaaatagg caagagctcg tgggtcgcaa cccgttaccg cgatcaggct
     1921 cgcggtcgcc aggaaaatga cggcggcaaa ccgcaacatg aaaattgatc gaggggtcat
     1981 ccttttcccc cctccttcct tgggtagttc acaggccggt cagcccggcc agggtccaga
     2041 ggcgcccccc tggcccgacc agaacggccg gcgaaacgat caaccgaatc aaccaggacg
     2101 cgagacccaa aaaaacggcc gcgatcacca aaccggccag aggttttgcc aggttctgaa
     2161 atccgacagg ctgattcgcg atcgcgcttg acatcatcca catcatcaag ggaacgctaa
     2221 agagagcgta gatacacccc gccggattgg ctttccagga acgatcgatc tgccctcgcg
     2281 ccaaccacgc atacgccgtg gtcatgccgc agcttgggca gagtcggccc gtcgcggtca
     2341 aaaacgcaca gggccgaagc cccagttgcg tatgagtgcc aaatccgcgt ggatccggct
     2401 ccaacgttct tgccagaatc aagagccctg caagcccagc cgcggcgacc acgagccaaa
     2461 agcgaattgg ccaacccagc ggttgacgat caaattgctg cggaatcaag gttggccctc
     2521 cgcttccaag acgagtcagc gcggaccgcg cccgaatgag agggctcaag ataggtcgtg
     2581 ggagattacc ccaaagacgg atcttcggca agctcaggtt gggaaggccg gttctcatcc
     2641 tcataatgag cgatcaattg attgatgtcc gactagccgt gcgccgtgat ctgttcttca
     2701 tcctatcgca ctacagctca ttgacccagg cttcggcttc gagcatggcg gcctgcatcc
     2761 gtcgctcgac ttccatacgg cacgactcga tcgcgtcgcg atcggcgtcg tgaggaacgg
     2821 aaacggcagc cgggacgaca caggcagccg gagtgaacgg tcgaggcacg cagaactggt
     2881 cccaactttt ggctctccac ggtcttttga acgccatgcc agcgcccacg atcggcaggc
     2941 cggtgcggct ggcgatatag gcgacgccct ggtggaccga tcgccggggt cctcggggac
     3001 cgtcgggcgt gacgcataga tgaccttgat cgatacgcag gctcatttca cggagagcgc
     3061 gcacaccgcc ccgtgtggtc gagccccgga ccacgctgaa ccccagccgt ttcacaacct
     3121 gggttatcag ctcgccgtcg cgatgttcgc tgatcaagat gtgcatctcg ggccagttcc
     3181 agtagtacgc tggaaagagc gtgacctcgt ggaaaaacgc gtagatgtag cgctggcctg
     3241 tcttacgggc gacttcgggg tcgacagtgg ggtctttata acgaaagtga tatttcaggg
     3301 tgcgccccag atgccagacc agggcagcgc ccgttgtccc gatcgcacgg accaagattg
     3361 gatgttggat cttcatggcc gcggcccgtc cccttccctg gcagacccag gactgagtat
     3421 cacggtaaac cgggggcagg ctatgcaaaa aggccatcga gggccagatc aagacgtggt
     3481 aaacctcatc tggcacccag cgcgcagcag gaaagcacgg tctatcgatt tccggccgcc
     3541 gccggcaggc gcgtcaaaga gtacctggtc tccgtggcgt tttgggatcg agcattggtc
     3601 gatccttctt tcgattagcc agcttcgccg ccggcaggcg aggggacggc tcatcgaggg
     3661 cccgcgggtg ggttctggta gcccggggac gcgcttcaaa aagccgggaa acaacatcgt
     3721 cgagcgtgtc taccaaccgg agcgtaaggt ccgccttgac ctcgggtggg atgtcgacca
     3781 gatctttctc attgtgccgg ggcaagagta cggtctcgat gccggcacga cgtgcggcca
     3841 ggattttctc gcgcactccc cccacaggca gcacacgacc cgtgagcgtc acctcgccgg
     3901 tcatcgcgag gttggccttg atcggtttat cgcgaaacaa gctgatgagg gccgcggcga
     3961 tggccacacc cgccgaggga ccatccttgg ggacggcgcc ggccgggacg tggatgtgca
     4021 agtcggtctt gccgaaccgc gaggcgtcga gcgacagcgc cttggcatgg ctgcgcaaat
     4081 agctcaaggc ggcctgagca ctctctcgca ttgactcgcc cagaagtccg gtcagggtca
     4141 accccccctt gcctgggctg gcgctcgcct cgataaagag gatctcgccc ccggtcgggg
     4201 tccaggctag tccggtggag actcccggaa tgccggtgcg gtcggccagc tcgagtaaat
     4261 aacgcgatgg acccaacaat tccgcgatgt ctttgggctg gacgacactc ggcgctcgtt
     4321 tgccttcggc ccgccgtcgg gccatcttcc ggcagatcga ggcaatctct cgttcgagat
     4381 tgcgcaggcc ggcctcacga gtgtaatcgg cgatgatctt gcggatcgac gcgtcgctga
     4441 tcgtcagatt agcgggcgcc agtccatgca aatcaagctg cttgggaatg atgtatttct
     4501 gggcaatatg tgttttctct tcctcgctgt atccgggcag ctgcaagacc tccagccggt
     4561 ccagtagagg cgacggaatc gtttccaaca tattggcggt ggcgatgaac attaccttgg
     4621 acaggtcgaa atcgacgtcc aggtagtggt cgcgaaaggt ggagttttgt tcggggtcga
     4681 gaacttccag gagggcggcc gagggatcgc cgcggaagtc ggcccctagc ttgtcgacct
     4741 cgtcgagcat gaaaac
//
LOCUS       DATAQL010001176         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2867146, whole genome shotgun sequence.
ACCESSION   DATAQL010001176 DATAQL010000000
VERSION     DATAQL010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2867146"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VHS97_20270"
     CDS             <1..653
                     /locus_tag="VHS97_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450602.1"
                     /translation="FRRYADKAAGDPALALDEYYDQGYDLMGSRQAQRAFDITREDPK
                     VRDRYGRSPFGQRCLLARRLVEAGVPFITLNEGGWDHHVSLFDKFDQRMPAVESSIAT
                     LIDDLDQRGLLDSTLVIALGEFGRTPKINKDAGRDHWSNAMSVLVAGCGTPGGQVVGA
                     TDARGYAASDRVLSPENFAATVYTKLGINPGKILYTPSGRPTYLVTDPTPIRELMG"
     gene            650..2296
                     /locus_tag="VHS97_20275"
     CDS             650..2296
                     /locus_tag="VHS97_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX3450603.1"
                     /translation="MNQRSQTIALFSAAVFVAGTTIAYAKPPTLSGFFPSGAARGQSL
                     TVTMSGTFDRWPVKCWVDGPGLSIEAGGDKGKLAICVAADAAPGVRWVRVYDEEGATS
                     LRPFIVGTLPEIVESEPNDDPQHPQSIGLASSTVNGRLSPSGDVDGYSVSLDRGQKLV
                     ADLEASRHLGSPMDAVLQVVSAAGFVLAQNDDAVGRDPRIIFEAPAQGTCIVRLFAFP
                     STPDSRIRFAGGDAFIYRLTLTTGGFLDHVFPLAVSQDSPTSLTAIGPNILEPDRALT
                     ICPNEERDVFTLFHPRLPGNTNVRRVTGNSVVEVEPNDLAQPQELSDRDSISGRIDPP
                     GERDVFRINLKKGDKHFFSLESRALGLPLDAVLQVLDADGKTLAETDDVGKSRDPELS
                     FTPPADGKYRVVVSDLNGRGGPHFAYLLRALVPEPDFNLSLSADRFELGSGKKTDIVV
                     KIDRSNGFTDTIDVVAEDLCKCVVATTARSRQSDATAKSVTLEIRSEGCSNAGPIKIV
                     GRSADGKSRLRIARAQIPGFEAKTDRPWLTILPVAEPKKP"
     gene            2324..>2767
                     /locus_tag="VHS97_20280"
     CDS             2324..>2767
                     /locus_tag="VHS97_20280"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3450604.1"
                     /translation="MRDDEPIPRTDVPAAGNTVVSPTCLALHGLGGGPYEIGPLIDAL
                     ETHGLTVEAPILPGHEPKGPVMPASCWCDWATASEAAFDALAAGGRPVVVIGFSTGGT
                     LALRLATRRPVARMILLAPFLAIRYSGLIPFRPASYLRYIARLTPN"
BASE COUNT          518 a          876 c          813 g          560 t
ORIGIN      
        1 acttccgtcg atacgccgac aaggctgcgg gcgaccccgc cctggctctc gacgaatatt
       61 atgatcaagg gtacgacctg atggggtctc gtcaggctca gcgcgctttc gacatcaccc
      121 gtgaagaccc gaaagtccgc gatcggtacg gccgaagccc atttggtcag cgttgcctgc
      181 ttgcccggcg tttggtcgag gccggtgtcc cgttcatcac tctcaatgaa gggggctggg
      241 accaccacgt ttcgctcttc gacaaattcg atcagcgaat gccggcggtc gagtcttcga
      301 tcgcgaccct gatcgacgac ctcgaccagc gcggattgct cgattcaact ttggtgattg
      361 cactcggaga gttcggccgg acaccgaaga tcaacaagga tgcgggccga gaccattggt
      421 cgaacgccat gtcggtactg gtcgctggtt gcggtactcc gggtggccag gtcgtcggtg
      481 cgacggacgc ccgaggatat gctgccagtg accgcgtcct ttcccccgag aacttcgcgg
      541 ccaccgtcta tacaaagctt gggatcaatc ctggtaaaat tctgtacact cccagcggtc
      601 gtcccaccta cctggtaacc gacccgaccc cgatccgcga gttgatggga tgaaccagcg
      661 atcgcagacc atcgcgctct tctccgccgc ggtcttcgtc gcgggaacca caatcgccta
      721 tgccaagcca cctacactga gcggattctt tccgtccggt gccgcacgcg gccaatcctt
      781 gacagtgacg atgtcaggga cgttcgatcg ttggccggtg aagtgctggg tcgacggtcc
      841 cggtctctca atcgaggccg gcggggacaa gggaaaactg gcaatttgtg tggccgccga
      901 tgctgcgcca ggcgtgcgtt gggtgcgcgt ttatgacgaa gagggagcga caagtctccg
      961 cccgttcatc gtcggtacgc ttccggaaat cgttgagtcg gaaccgaacg acgacccaca
     1021 acatccgcag tcaatcgggc tcgcatcgtc cacagtcaac ggccgactct ctccgtcggg
     1081 tgatgtcgat ggctactctg tcagcctgga tcgcggacag aaactcgtgg ccgacctcga
     1141 agccagccgc catctcggtt cgccgatgga cgccgtgttg caggtcgtct cagcagccgg
     1201 cttcgtgctt gcccagaacg atgacgccgt tggccgcgac ccacggatca ttttcgaggc
     1261 accggcgcaa ggaacttgta tcgtccgcct cttcgccttt ccttcgacgc ccgacagcag
     1321 gatccggttc gcggggggag acgccttcat ctatcgtctc acactcacga ccggtggctt
     1381 cctcgaccac gtctttccgc tcgccgtctc gcaagacagc ccgacctcgc tgaccgcgat
     1441 cggcccgaac atcctcgaac ccgaccgtgc actgaccatt tgtcccaacg aggagcgtga
     1501 cgtgttcact ctctttcacc cccggttgcc agggaatacc aacgttcggc gcgtcacggg
     1561 caattcggtc gtcgaggtcg aacccaacga tctggctcag ccgcaagagt tgtcggaccg
     1621 cgactcgatt agtgggagaa tcgaccctcc cggcgagcgg gacgtctttc ggatcaattt
     1681 gaaaaagggc gacaaacact tcttctctct ggaatctcgg gctcttggcc tgcctctgga
     1741 cgctgttctc caggtgctcg acgccgatgg caaaacgctg gccgagaccg acgacgtcgg
     1801 caaatcacgc gatcccgagc tttccttcac gccccctgct gacggcaagt atcgtgtcgt
     1861 cgtaagtgac ttgaatgggc gagggggacc acactttgcg tatctcttac gggcgctcgt
     1921 cccggagccc gactttaacc tgtcactgtc ggccgacaga ttcgagttgg gatcgggcaa
     1981 gaagacggat attgtcgtga aaattgatcg cagtaatggt ttcaccgata cgatcgatgt
     2041 ggtcgccgaa gatctctgca aatgcgtggt agcgacgact gccagatcgc gtcagtcaga
     2101 cgccacggca aagtcggtga cgctcgaaat ccgctccgaa gggtgttcca acgctggacc
     2161 gataaagatt gttggaagat cggccgacgg aaagtcacgc ttgcggattg cccgcgcgca
     2221 gatcccgggg ttcgaagcca aaacggatcg cccttggcta acgattcttc ctgtcgccga
     2281 gccaaagaaa ccgtaactcg tcgagacgga agcgctggga cacatgcgcg atgacgagcc
     2341 tattcctcga accgacgtcc ccgccgccgg caacaccgtt gtgtcgccca cttgcctggc
     2401 cctgcatggg ctggggggcg gtccctacga aatcgggcca ttgatcgacg ctctcgagac
     2461 gcatggtctc accgtcgagg cgcccatctt acccggccat gaacccaaag gtcctgtaat
     2521 gccggcctcg tgttggtgcg actgggcgac ggcatccgag gcggccttcg acgcgctggc
     2581 ggcgggcggc cggccggtgg tcgtgatcgg tttttcgaca ggcggtacac tcgccttgcg
     2641 tctggctacc cgccgcccgg tcgcgcgcat gattttgctc gcgccgttct tggcaatccg
     2701 ctatagcggg ctgatcccgt tccgcccggc gagttacctc cgatacattg ccaggttaac
     2761 ccccaat
//
LOCUS       DATAQL010001177         4732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3476917, whole genome shotgun sequence.
ACCESSION   DATAQL010001177 DATAQL010000000
VERSION     DATAQL010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3476917"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VHS97_20285"
     CDS             complement(<1..1156)
                     /locus_tag="VHS97_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3450605.1"
                     /translation="MTRRQLRTLWSAPAFPLALASLLLAAHCRADDGKTVVDRAAPTL
                     LSSPANPLVALRVVFRAGSQDDPPGKEGLAALTAAMVAEGGTKSLSYEQVLEAFYPMA
                     AMLGGECHKELTVFSGTIHRDNLAAYIPLVTDMITQPRFAAQDFERLRNEALDYITKS
                     LRGNDDEQLGKWTLQVEIYKDHPYGHPDRGTAEGLRSITLGDVKEFHRRHYTGGALKL
                     GLAGGFSETTPGLCDRAFAGLPATRLEIPALPSPGMPKGLEVTIVEKPADATAISIGF
                     PIDVTRRDDDFYALAVANSYLGEHRTFNGKLMQDLRAKRGLNYGDYSYMEDFIQDRMT
                     NFPVPNNPRRQQAFTIWVRPVPRDKAAFALRGALWELDRLVDKGMSPEDFE"
     gene            complement(1153..2511)
                     /locus_tag="VHS97_20290"
     CDS             complement(1153..2511)
                     /locus_tag="VHS97_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3450606.1"
                     /translation="MRRLTALAVLAVLTGPPVGLAAGPPSPGSKPVFTYPIQKAVLEN
                     GLTVYSVPFDSPRIIAYYTVVRTGSRNEVEPGLSGFAHFFEHMMFRGTESYPQEKYND
                     VLKSLGADSNAFTSDDLTCYHMTAPATALATAVEIEADRFQNLKYDEPSFQKEARAVL
                     GEYNKSASSPFLKLNETMQDTAYTTHTYKHTTLGFLTDIRNMPNQYAYSKVFFDRWYR
                     PENCTIVVVGDVVHDALVSLVKQYYGGWKQSKSQVEIPAEPPQSKPKSARLTWPLPTL
                     PILTLGYHIPAADPAHPDTAALGALVEAVFGEASALYQELVLKEQKVVMIMAEAESKR
                     DPGLFSIFVQVRKSEDLPIVRARIAAALAGAAKTPVDSARLDAVKSHLRYRFAGSLST
                     ADAVAGALSAAIAVTGRPESINELYDAYDRLTPADLKRVAAKYFQPNNETAITLETEL
                     KK"
     gene            complement(3384..4469)
                     /locus_tag="VHS97_20295"
     CDS             complement(3384..4469)
                     /locus_tag="VHS97_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3450607.1"
                     /translation="MITRYCKWCFLTAIVWGMGSIAVAQPAATGPFEIYFIDVAGGAA
                     TLLVTPERESVLIDSGWPGLNDRDPKRIVHVLRDLAGCDQLDHLVTTHWHLDHFGGVA
                     GLARLIKIGRFWDRGLPEDGDSRLDFPDGPKPDDPLGIAYRSASAGKRKALKAGDLLP
                     LKGVRARILASGGSVIDPATIGLDTADAAKRANPLCETAPPDRAVDPSDNARSLAILF
                     SLGKFQFFDAGDLTWNVEKKLVCPVDLIGPVDLYQVTHHGMDNSNHPTLVRTISPSVA
                     IMNNGPNKGGAPGTVKLLKSIPSIQAAYQLHRNAATRDEDNTAPSLIANKDAAGGEFI
                     RVRVAPDGAKFTVQIGEHGAERTFESN"
BASE COUNT          955 a         1439 c         1409 g          929 t
ORIGIN      
        1 cctcgaagtc ctcggggctc atccccttgt cgacgagccg gtcgagctcc cacagggccc
       61 cgcggagggc gaaggccgcc ttgtcgcgcg ggacggggcg gacccagatc gtgaacgcct
      121 gctgccgcct cgggttgttc ggaacgggaa agttggtcat gcgatcttga atgaagtctt
      181 ccatatacga gtaatcgcca taattgagcc cacgtttggc gcggaggtct tgcatcagct
      241 tgccgttgaa ggtgcggtgc tcgcccaagt aagaattagc aacggccagg gcgtagaagt
      301 cgtcgtcgcg gcgggtcaca tcgatcggaa agccgatcga gatcgccgtc gcgtctgccg
      361 gcttttcaac gatcgtgacc tccaggccct tgggcatgcc cggggacggg agcgccggaa
      421 tttcgagtcg ggtcgcgggg agaccggcaa acgcgcgatc gcaaagaccg ggcgtcgtct
      481 cactgaagcc accggcaaga cccagtttga gggctcctcc ggtgtagtgc cggcggtgaa
      541 attccttgac gtcgcccaag gtgatcgacc tcaagccttc ggcagtcccg cgatcgggat
      601 gaccataggg atgatccttg tagatctcga cctggagcgt ccatttgcca agctgctcgt
      661 cgtcgttgcc gcgcagcgac ttggtgatgt aatcgagcgc ttcgttgcgt agcctttcaa
      721 aatcttgcgc cgcaaaccgc ggctgggtga tcatgtcggt cacgagcggg atatacgctg
      781 ccagattgtc gcgatggatg gtacccgaaa atacggtcag ctccttgtgg cattcaccac
      841 cgagcatggc cgccatcgga taaaacgctt cgagaacctg ttcgtagctg agcgatttcg
      901 tcccgccttc ggcgaccatg gcggcggtca atgcggccag accctccttg cccggcgggt
      961 cgtcttgaga accggcgcga aacacgacgc gcagcgccac cagcgggttg gccggcgagc
     1021 tcaggagcgt cggcgccgcc cgatcgacca cggtcttgcc gtcgtcggcc cgacaatgag
     1081 cggccaacaa aaggctcgcc aatgctaagg ggaaagccgg tgccgaccaa agggtgcgaa
     1141 gttgtctcct ggtcatttct tgagctccgt ctccaaggtg atggctgtct cattattggg
     1201 ctgaaagtac ttcgccgcca cgcgcttcag gtcggcggga gtgagacggt cgtaggcgtc
     1261 gtacagctcg ttgatcgatt cgggacggcc ggtgacggct atggccgcac tgagcgcgcc
     1321 agcgacggcg tcggcagtgc taagcgaacc ggcgaaccgg tagcggagat gagacttgac
     1381 ggcatcgagt cgcgccgagt cgaccggagt cttggctgct ccggcaaggg cggcggcgat
     1441 ccgtgcacgc acgatcggca gatcctccga tttccgaacc tggacaaaga tggaaaagag
     1501 accggggtct cgcttcgatt cggcctcggc catgatcatc acgacttttt gttccttcaa
     1561 gaccagctcc tgatagagag cactggcctc gccgaagacc gcttccacga gcgcacccaa
     1621 ggcggccgtg tcgggatggg cggggtccgc cgccggaatg tgatagccca gggtaaggat
     1681 cggcaacgtg ggtagaggcc aagtcagccg cgcggacttc ggcttcgact ggggtggctc
     1741 ggcgggaatc tcgacctgcg acttgctctg cttccagccg ccgtaatatt gcttgaccag
     1801 cgacaccaag gcgtcgtgca ccacgtcgcc gaccacgaca atcgtgcaat tctccgggcg
     1861 gtaccagcgg tcgaaaaaga ccttgctgta tgcatactgg ttgggcatgt tgcggatgtc
     1921 ggtcaagaat ccgagcgtcg tgtgcttgta ggtgtgggtc gtgtaggccg tgtcttgcat
     1981 cgtttcgttg agcttcaaga acggcgacga tgcgctcttg ttgtattcgc cgagtacggc
     2041 ccgagcctct ttctgaaacg acggctcatc gtacttgagg ttctgaaatc ggtcggcctc
     2101 gatctctacc gccgtcgcca acgccgtcgc gggagctgtc atgtgataac aggtcaggtc
     2161 gtcgctcgtg aaggcgttgg aatccgcccc cagcgacttg aggacatcgt tgtacttctc
     2221 ttgcgggtac gactcggttc cccgaaacat catgtgctcg aagaaatgtg cgaaacccga
     2281 aagccctggc tcgacttcgt tgcgcgagcc cgtgcgaacg accgtgtagt acgcaatgat
     2341 tcgaggcgag tcgaacggta cggaatagac tgtcaggcca ttttcgagca ccgctttttg
     2401 gatcgggtac gtgaagaccg gcttgctgcc gggcgacggc ggtcctgccg ccaacccgac
     2461 cggcggcccg gttaacacgg ccagaacggc cagagctgta agtcgtctca ttgatggtgc
     2521 atcctccttg gagaatccgg aacgtcactc ccggttgact tcaaaacatt cattcaaaat
     2581 aaccttcatt gaccgcccag cggttaacgg caagccggtt cctcgccagc gtatcatgac
     2641 atacagcaaa atgcccgccg ccatccagcc caaaccgagg gctcgtccca gccactcgat
     2701 ccagccacgc tccgggcgat cagggacgtc agcatacctg gctgccagaa caaatgccgg
     2761 agcggcggcc tcggctggcg aacgcgcaaa atcatataag ttggagtcaa cgtgaaaatc
     2821 agagagttaa aacagaacac gaaaaacgaa accgcgaagc tcttattcaa tccattcgag
     2881 gcgacaggcc gctggacacg gacatgttga aacgcgaaat ctcctagcaa gtggcgtgtc
     2941 tgtacgaacc agaccgacat gcccacgagc acgatcattc cgtccagaat cgtgaatcgg
     3001 cggcagggcg tactctccac cgagtctcga tggagttgat cggattcttc catctcgtca
     3061 ctcgctcgct ctcgggacgc ctccaagcaa ccgtagtcga ccgcggcacg gggtgtcgca
     3121 gccgcaaggg tggctacgcg accaccactc gcaccgtgaa ggacttacgg gccactgaaa
     3181 aaaggccatc cacgaggttg aatcgccgga ccgccccagg gttcgccagc cgcttccccg
     3241 gaccggacct ggcatgccgg gcgcctctca cctcgcgtcg aacgtccgtt caccaccttg
     3301 ctcgccaatt tgcaccgtga aactggcgcc atccggcgcg gcccgctgcc gcaaccgaat
     3361 gacggccccg tagtgtacca acttcaattc gactcaaacg tcctctcggc accgtgttcg
     3421 ccgatttgca ccgtaaattt ggcaccatcc ggcgcgactc gaacgcgaat gaactcgccg
     3481 cctgctgcgt ctttattggc gatcaatgag ggagcggtgt tgtcttcgtc gcgggtggcg
     3541 gcgttccggt gaagctgata ggccgcctgg atcgagggaa tcgatttgag caatttgacc
     3601 gtgccgggtg ctcccccttt gttcggcccg ttgttcatga tcgccaccga cggcgaaatt
     3661 gtccgcacca gggtgggatg attcgagtta tccattccat gatgcgttac ctgatacaaa
     3721 tcgaccggcc cgatcaggtc gacagggcag acaagcttct tttccacatt ccatgtgagg
     3781 tcgccggcgt cgaaaaactg aaatttgccg agcgagaaca agatcgccag gctgcgtgcg
     3841 ttgtctgatg ggtcgacggc ccggtcgggc ggagccgtct cacacagcgg atttgcgcgc
     3901 ttggcggcgt cagccgtgtc gaggccgatg gtggccgggt cgatgacgct accccctgat
     3961 gccagtattc gagctcgcac gcccttcagg ggcaacaaat cgccggcctt gagtgccttg
     4021 cgcttgccgg cactcgccga gcgataggcg attcccagcg gatcgtcggg cttgggcccg
     4081 tcaggaaaat ccagccggga atcgccgtcc tccggcagac cgcggtccca aaaacggccg
     4141 atcttgatca aacgggccag gcccgccacg cccccaaaat ggtccaggtg ccagtgcgtg
     4201 gtcaccagat gatcgagttg gtcgcatccg gccaggtccc gaagaacatg aacgatgcgc
     4261 ttgggatcgc gatcgttcag tccgggccag ccggaatcga tcaaaaccga ctcgcgctcg
     4321 ggggtgacca aaagcgttgc cgctcctcct gcaacgtcaa tgaaataaat ctcgaacgga
     4381 ccggtcgccg ctggctgcgc aaccgcaatg cttcccattc cccacacgat tgctgtcaga
     4441 aaacaccatt tgcagtatct cgtgatcatc gttgtttgct ccccgcaaat cgaactagtc
     4501 agcggaaaat tgcattctcg cgtaagacca atagatcacc ccgccccaac cgcggccgtc
     4561 aagacctgcc gatcgcggtt gtcgactgct gcaacccgcc gggaccacgc ctgagcccag
     4621 aacagatctc attcaatcac acaggctttc gtcagcgaat cgctatcgca ccctgagcat
     4681 tgaccaatcc tcggtctctt agagtgagtg tcaatttttg tgacactctg tt
//
LOCUS       DATAQL010001178         3944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2412120, whole genome shotgun sequence.
ACCESSION   DATAQL010001178 DATAQL010000000
VERSION     DATAQL010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412120"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHS97_20300"
     CDS             <1..208
                     /locus_tag="VHS97_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450608.1"
                     /translation="SLIDDRMFADYLTDWDVTVIPGSCFGLAPFFRISYAASEQDLIV
                     ALDRIARACASLDLEQAQVTAVRR"
     gene            494..1843
                     /locus_tag="VHS97_20305"
     CDS             494..1843
                     /locus_tag="VHS97_20305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3450609.1"
                     /translation="MVSSVQYGEDGTTDQPTAFPWVVCAVLASFNALSYMDRLLLGLL
                     SLPLQRDLHIDDVQFGLLQGLAFSIIYVACSPAAGWATDRFSRRTMVFAGVLVWSLAT
                     MSCGHANTFYQLFLSRSLVGVGEAMLAPAAFAILAEQTPPGRLGIAMSIYAIGAGLGV
                     VGALTGGGAAISAIPKEGVTIPLFGHLQPWQFAFVLLGAPGMFLAFLAFVLPGRTARP
                     SAGRSRLSECDEQPSFWRFLVTRRGALFCQQIGFSMLGLAGYAIVGWAPAFFGRAFGW
                     SPAKAGPLLGVAVGVGGLIGMSVFGAAADRRFRQGDDSAYFRTHMITTLCGAPIIVLA
                     FLVHQPWLCMGLLMLGFPLLYSFGGSATAALQLITPSALRGRAGGLYLFTINVLGLGI
                     GPLAVGTLTDKVFASPRMVGASVAVTVAVSSAIAVVCLALGQGAFRQAMRLTRGPAV"
     gene            complement(1940..2374)
                     /locus_tag="VHS97_20310"
     CDS             complement(1940..2374)
                     /locus_tag="VHS97_20310"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX3450610.1"
                     /translation="MSRSGRISWADAVVGQHLDAMVTRPLTITDFVRYQGASGDMNPI
                     HHDVEFAQKAGYPGPFAVGMRQAGVMAGYCAELFGPENVRRFKVQFREQAWPGDIITY
                     KATVAEKRVEDGVRLVDLELVAERQTGGVHLKGWATFVLPEK"
     gene            complement(2371..2838)
                     /locus_tag="VHS97_20315"
     CDS             complement(2371..2838)
                     /locus_tag="VHS97_20315"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3450611.1"
                     /translation="MAESKAEATGEAPRSEGRGQPFDFWVETGKVREFARATGTKDPA
                     YAEAQPVSPPTYLMAASFWQTAESAPWGDSPPDFRRLLHGEQEFVFHGPPPKAGDHLV
                     GEVRIDNRYSKPGKRGGIMNFTEMVTEYRDADTKELVAEVRATLIETSKAATA"
     gene            complement(2930..3757)
                     /locus_tag="VHS97_20320"
     CDS             complement(2930..3757)
                     /locus_tag="VHS97_20320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3450612.1"
                     /translation="MGKLNGKSAIVTGGGRGIGRSVALLLAAEGARVVVNDLDQEPAN
                     QVVAEIRKNGGEAVACPGSVTSEGFAKRFVQTAVDNYGGIDIIINNAGYTWDSVIQKM
                     TDEQWYAIVDLHLKAPFDILRAAQPVISAAVKKEKSEGRVVHRKVVNISSVAGVFGNA
                     GQANYSSAKAGIVGLTKTLAKEWGRYNVNVNCVAFGFILTRLTEATADQESTIEVEGR
                     AIKVGVNPQVLERVKAMIPLGRPGTPEEAAGSVVMLTYPEADYVSGQLLVTGGGLEG"
     gene            complement(3757..>3944)
                     /locus_tag="VHS97_20325"
     CDS             complement(3757..>3944)
                     /locus_tag="VHS97_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450613.1"
                     /translation="VRSIHDVDGRRLATLDLAVTLADGTTTLVGEAVVEINDEEQVRR
                     ASSTASGVRMAQSMEGR"
BASE COUNT          625 a         1289 c         1191 g          839 t
ORIGIN      
        1 ctcgctgatt gatgatcgca tgttcgcgga ctatctgacc gactgggacg taacggtgat
       61 tcctggatcc tgctttggtc tcgccccgtt ctttcgcatc tcatacgcgg cgtcggagca
      121 ggatctgatc gttgcgctcg accgcatcgc aagagcttgc gccagtctcg atcttgagca
      181 agcccaagta acggctgtaa ggcgctgaag cggtatgacg cggggggagc cttgcaatat
      241 ccagtattgt taggaaaggc cgccagcgac agtcaggctg cgtaaccatt cgatgattgc
      301 cggttcatcg aagaaagatg tgagcagatg ttactcgccg atacagcacc tgttcgcagc
      361 atgcgaccag acctcaaaaa gtatttttgt cgataggcgc gtccgcgtgc gctccacggc
      421 ggtagatcgc aggtccggca agcgctcgcg gacggatccg acggcaacgc agctgcggtg
      481 atcagaggaa acaatggttt caagtgtgca gtatggcgag gacggaacta cggaccaacc
      541 gacggccttt ccctgggtag tgtgcgcggt cctcgccagc ttcaacgccc tgtcttacat
      601 ggaccggctg ctcctcggcc ttttgtcgct cccgctgcaa cgcgacctgc atatcgacga
      661 cgtgcagttc ggtctcctgc aagggctagc cttctcgatc atctacgtgg cctgcagccc
      721 ggcggccggt tgggcgacag accggttctc acggcgcacg atggtgttcg ctggcgttct
      781 ggtttggtcg ctggcgacca tgagctgcgg tcacgccaac accttctacc aattgtttct
      841 gtcgcgatcg cttgtgggcg tcggcgaggc catgcttgca ccggcggctt tcgcgatcct
      901 ggcggaacag acgccaccgg gccggctggg catcgccatg tcgatctatg caattggcgc
      961 aggcttggga gtagtgggcg ctctgacggg cgggggggcg gcgatctctg caattcccaa
     1021 ggagggcgtg accatccctc tgttcgggca tttacagccc tggcagttcg ctttcgttct
     1081 gctcggcgcg cccgggatgt tcctagcctt cctcgccttc gttcttccgg ggcggacggc
     1141 gaggccgtca gccggccgat ctcgtttgag cgaatgcgac gagcagccga gcttctggcg
     1201 cttcctcgtc acccgtaggg gcgccctgtt ttgccagcag attggctttt ccatgctggg
     1261 actcgccggc tacgctatcg tcggctgggc gcccgccttc ttcggccggg cctttggctg
     1321 gtcccctgcc aaggctggac cgctcctggg agtggcggtt ggggtcggcg gcctaatcgg
     1381 gatgtcggtg tttggcgccg cagctgaccg gcggttccgg cagggtgacg attcggccta
     1441 cttccgcacg cacatgatca ctacgctttg cggcgctccg atcatcgtgc tggccttcct
     1501 cgttcaccag ccgtggcttt gcatgggcct cctcatgctc ggcttccccc tgctgtacag
     1561 cttcggcgga tcggcgaccg cggcgctcca gctcatcaca ccttccgcgc tgcgcggacg
     1621 ggcgggaggg ctctacctgt ttacgatcaa cgtgctcggc ttgggcatcg ggccgttggc
     1681 ggtgggaacg ctcacggaca aggtgttcgc aagcccccgg atggtgggcg cttcagtggc
     1741 cgtgaccgtg gcagtttcat cagcgatcgc ggtcgtgtgc ctggcgctcg gccagggagc
     1801 ttttcggcag gcgatgcggc tgactcgtgg gccagcggta tgaatcggtg atatgaattg
     1861 ggctcatgga gccgcccgcg cttcgcgaac acctcggggg aaaactcttc cccgccactt
     1921 cgaaccgagg gcgtcaggcc tacttctccg gaaggacgaa ggtcgcccaa cccttgaggt
     1981 ggacgccacc ggtctgccgt tcggcaacca actcgaggtc gaccagccgg accccgtcct
     2041 cgacgcgctt ttcggccacg gtcgccttgt aggtgatgat atcgccaggc caggcctgtt
     2101 cgcggaactg gaccttgaac cgacgaacat tctcgggtcc gaacagctcg gcgcagtagc
     2161 cggccatcac cccggcttgg cgcatgccga cggcgaacgg cccgggatat ccggccttct
     2221 gtgcgaactc gacatcgtga tgaatggggt tcatgtcacc cgacgccccc tggtaacgga
     2281 cgaagtccgt gatcgtgagc ggccgggtga ccatcgcgtc aaggtgctgg ccgacgaccg
     2341 catcggccca cgaaatccgt ccggaccggc tcatgccgtg gccgccttgc tcgtctcgat
     2401 cagcgtggcc cggacctcgg ccacgagttc cttcgtgtcc gcgtctcggt actccgtcac
     2461 catctcggta aagttcatga tccctccacg ctttcccggc ttcgaatacc ggttgtcgat
     2521 acgcacctct ccgactagat ggtcgccagc tttcggcgga ggtccgtgga acacaaactc
     2581 ctgctcacca tgcagaagac gtcggaagtc cggcggcgag tcgccccaag gtgcgctctc
     2641 ggccgtctgc cagaagctgg ctgccatcag gtacgttggt ggcgaaaccg gctgtgcctc
     2701 cgcgtaggct ggatccttcg tccccgttgc ccgggcgaat tcccggacct tgcccgtctc
     2761 gacccaaaag tcgaagggct ggccccgtcc ctcggatctc ggggcctctc ctgtcgcctc
     2821 cgcttttgac tcggccatca cccctcctct tctgactcca gtcgcggtcc gaagaccgct
     2881 taccacgtct tgtttatagt cgttctgcgg acgacgtcgc atcggcaaat tagccctcga
     2941 ggccgcctcc ggtcacgagc aattgaccgg agacgtagtc cgcttcgggg taggtgagca
     3001 tgacgaccga gccggcggcc tcctccggag tacccgggcg accgagcgga atcatggcct
     3061 tgaccctctc gagaacctgc ggattgacac cgaccttgat tgcacgacct tcgacctcga
     3121 tcgtggactc ttgatctgcc gttgcttcgg tcaatcgcgt caggatgaag ccgaaggcca
     3181 cgcagttcac gttcacattg tatctccccc actccttggc gagagtcttc gtcagtccga
     3241 cgatgcccgc tttcgcggag gagtagttgg cttggccggc gttgccaaac acacccgcaa
     3301 cggacgagat gttcactacc ttgcggtgca caacccgtcc ctccgacttc tccttcttca
     3361 ccgcagcact gatgaccggc tgcgccgcgc gaaggatgtc gaacggtgcc ttcaagtgca
     3421 gatcgacgat cgcgtaccac tgttcatctg tcatcttctg gatgaccgaa tcccaggtgt
     3481 atccggcgtt gttgatgatg atgtcgatgc cgccgtaatt gtcgaccgcc gtctggacga
     3541 atcgcttggc aaacccttcg ctcgtcacac tccctggaca ggcgaccgct tcgccgccgt
     3601 tcttcctgat ctccgccacg acctggtttg cgggctcctg gtcaaggtcg ttcacaacca
     3661 cgcgcgcacc ctccgcggcc aacagcagcg cgaccgacct gccgatcccg cgcccgccgc
     3721 cagtgacgat cgcggacttc ccgttgagtt tccccatcat cttccctcca tgctctgtgc
     3781 catgcgcacg ccgctcgcgg tcgaacttgc tcgacggact tgctcctcgt cgttgatctc
     3841 gacgacggcc tccccgacga gagtcgttgt gccgtctgcc aacgtgacgg cgaggtcgag
     3901 cgttgccagc cgtcgcccgt cgacgtcatg tattgaccgg acgg
//
LOCUS       DATAQL010001179         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_272599, whole genome shotgun sequence.
ACCESSION   DATAQL010001179 DATAQL010000000
VERSION     DATAQL010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272599"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1984
                     /locus_tag="VHS97_20330"
     CDS             371..1984
                     /locus_tag="VHS97_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3450614.1"
                     /translation="MGDTRLAWALACAGVLAASALARPCRAQGPTIQETGIITRGGIT
                     TTPGSLNSLLGMLPGSSIATFGTQPGRDDLLLGRIGTGTPRVPTSITMPGGTYQGPQA
                     GRVTAPLQPLPVPQPLLYGSLELPKDEGDEGPPDGLTIDQAIDLLVRQNLDLRAKFLE
                     IPQARADVLTASLRANPIFFADSQLVPYGVDSVRRPDGPTQYDVNISHPIDYSHKRLA
                     RMAYASRAVEVMEAQYQNEVRLAIQNVYIAYVDVLTARETVRYLETSIKGLGEVLIAN
                     ERLYAEKTASRADVNQAKADRDIAVAGVMDAQEAVRQRTRVLGELLNLPAEEAERLEL
                     KGTIGDLAPPPPPQPELRQLALAARPDVMAFRLGITAAQANVGLQRASRFSDAYLLYQ
                     PYTWQNNAPFGKQSGASWAVGITVPLPVYNRNQGNIERAKINVYQTEVQLALQERRVE
                     TEVFQALKEYQVSGQIARHIKEQIMPALETAYRDRLRLFQEGEVTKFVFLDSQRKYND
                     MAKAYLDAAVRHRRSMLNLNTVVGQRILP"
     gene            complement(2166..2522)
                     /locus_tag="VHS97_20335"
     CDS             complement(2166..2522)
                     /locus_tag="VHS97_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3450615.1"
                     /translation="MATKTSVKSAPAPKTTTAKSKAFRGAEQRYHQARRASILLKHVS
                     DPTRLQVILILSEGERHVGALCAQLSQSQPAVSHHLALLRHGGIIAPRRQGKNNFYSL
                     TDTGTDLAHVVKNLIA"
     gene            complement(3080..3913)
                     /locus_tag="VHS97_20340"
     CDS             complement(3080..3913)
                     /locus_tag="VHS97_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3450616.1"
                     /translation="MTDYTLKGVVKRVESELEHVTIAHEAIPGFMDAMRMRFAYKNKN
                     TLATLRPGDLVEGTLRVERALGAVRNYELSDLQVLKPAPSVQAAPNISKNKALVGPQH
                     RLLAVGEPAPDFTMTNQDGQAVKLSDLRGNVVALTFIYTRCPLPDFCPLMDKKFAELA
                     QGIALYPERARQIRLISLSFDPEHDTPEVLRKHAQIRGATPPLWTYAVASHAELAKIG
                     VPLGLFYQPGDTEIAHNLATAVIDQEGNLARLEIGTERNKWDSTDMRKTLYSLLPPAP
                     K"
     gene            complement(4072..>4393)
                     /locus_tag="VHS97_20345"
     CDS             complement(4072..>4393)
                     /locus_tag="VHS97_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX3450617.1"
                     /translation="ETSMGLAHFPRLVALEQADSGAVKPSRFEAVNRGWVEITRPWHL
                     LTTNSGAGNPRAATAAKGEQFTTVVAERLGRFLTELAQSPLDDTFPFQNTLAPSTGPE
                     DKQI"
BASE COUNT          908 a         1210 c         1350 g          925 t
ORIGIN      
        1 gtcggttctc ctttcgtgtt gatagcgagc atattaacgc acaaaactag gcggcgacag
       61 tttctggcga aatttcgatg acgtcaaaga atgagaacga acactgcggc cgtttacgaa
      121 aaaccggcgc cgtaactcca atcagctcag aactgcggga tctaacgatt atgccgagtc
      181 tagcaaattt aaccaaattg agtagttttg gtaaaatcgc ttcaactacc aatctgggtg
      241 aagccgataa aactgcgagt acgggtgcga ctgccgaatg tgatctcgtc gccgccctta
      301 ggtgcgttct cttggtgacg gtcagagcag cggcgggcgt gagccgatat caaggggagg
      361 aacggaagcc atgggggaca cccgactcgc ctgggcactg gcgtgcgccg gggttctggc
      421 cgcgagcgcg ctggcgcgac cctgccgggc tcaggggccg acgatccagg agaccggaat
      481 tatcacccga ggtgggatca cgacgacacc tggttcgctc aactcgctct tgggaatgtt
      541 gccgggctcg agtattgcga cattcggcac gcagccaggg cgtgatgatc tgttgctcgg
      601 tcggatcggg acggggacac cgcgcgtgcc gacgtcaatc acgatgccgg ggggcacata
      661 tcaagggcct caggccgggc gggttaccgc gccgctacag cccttacccg tgccgcagcc
      721 gttactttac ggctcactcg aattgcccaa ggacgagggg gacgaaggac cccctgatgg
      781 cctgacgatc gaccaggcca tcgatctctt ggtgcggcaa aacctcgacc ttcgagccaa
      841 gtttctcgag atcccacagg cacgcgccga cgtcttgacc gccagtctgc gcgccaatcc
      901 gatcttcttt gctgacagcc agctcgtacc ttatggcgtc gactcggtgc ggcgaccgga
      961 cggtccaact cagtacgatg tgaatatatc acatcctatt gattattcgc acaagcggct
     1021 ggcccgaatg gcgtacgcat cgcgtgcggt cgaagtgatg gaagctcagt atcaaaacga
     1081 ggtgcgtctg gccattcaga acgtctatat cgcatatgtc gacgtgctga ccgcgcgtga
     1141 gacggtgcgc tacctagaga cgagcatcaa gggacttgga gaggttttga tagcgaacga
     1201 acgtctctac gcggagaaaa ccgcctcgag ggccgacgtc aatcaggcca aggccgaccg
     1261 cgatattgcc gttgcggggg tgatggacgc ccaagaagcc gtgcgccagc ggacgcgtgt
     1321 tctcggtgag ttactcaatc tcccggctga agaggccgag cggcttgagc ttaagggcac
     1381 gatcggcgac ctggcaccgc cgccgccgcc tcagccggaa ctacgacagc tcgcgctggc
     1441 ggcccgtcct gatgtgatgg cgtttcgact gggtattacg gccgcgcaag ccaatgtcgg
     1501 gctccagcgc gccagccgct tttcagacgc ttacctcttg tatcagccgt acacctggca
     1561 gaataacgcg ccttttggca agcaaagcgg tgcgtcgtgg gcggtaggca tcacggtgcc
     1621 gctgccagtc tataaccgca accaaggcaa catcgagcgc gccaagatca acgtttacca
     1681 gaccgaggtg caacttgcat tgcaagagcg aagggtcgaa accgaggttt tccaggcact
     1741 caaggaatat caagtgagcg gtcagatcgc ccggcacatc aaggagcaga tcatgccggc
     1801 gctcgaaacg gcgtatcgag accggcttcg gctcttccag gagggtgaag ttaccaagtt
     1861 cgtgtttctc gactctcagc gcaagtacaa cgacatggca aaggcgtatc tcgatgcggc
     1921 tgtgcgccat cgccgcagca tgctgaactt gaacacggtt gtcggccagc gaatcttgcc
     1981 atgaacgacg gcctcaccga gcggggcagg tgtgtacgcg ggagtggtga aggaggtgat
     2041 ccggagggcc ctcttgggcc gtccggacga ccacgaaaac ccggttgtgc ttgatagttt
     2101 attgaagagg tctaacgtgt attgactatg aacacacgtg aacagctagc cgcaatgata
     2161 gatgatcagg caatcagatt cttgacaacg tgggcgagat cggtgccggt gtcggtcagg
     2221 ctgtagaagt tattcttacc ctggcggcga ggggcgatga tgccgccgtg ccgcaagagt
     2281 gccaggtgat ggctgacggc gggctggctc tggctaagct gagcgcacaa ggcgccgacg
     2341 tggcgttcgc cttcggaaag aatcaagatg acttgaagcc gggtcggatc gctcacgtgt
     2401 ttgagcaaga tggatgctcg gcgagcttga tgatatctct gctcggcgcc gcggaaggcc
     2461 ttggacttcg cggtggtcgt cttgggggct ggggcactct tgacacttgt cttggtggcc
     2521 atggaaagac cctcctcgat gaattagttc tagttgcgcg cggtaaagca cccgcatccc
     2581 gagtccattc tcggaattcg cgggaataca aacggcccca cgaggatctt cttgtcctcg
     2641 ggtaacccag tcttggtcca ctctatggcg gctgcgactt cgaggaccag accggaaacc
     2701 aaacttacac cgcaggcttc ctagctgcct acgtggtcgc cggattggct ctaagtcaag
     2761 aaagcacaac caccaatggc cacgtcgatc gatcgacacg taaccgcccg caatctttca
     2821 acgacgccat cacgatcttg cgccaacgta tctcaacgtg ggtggttgtc gacatcttag
     2881 agcttttcta acaactgtca accgtaattt gcaatctttt agcgctttga ttcgtcacac
     2941 ttgttcgagc gagcgggttc catgcgcggc atgtctagaa tgctttaggg cggcgccatt
     3001 gatccgcaaa tatgtgcgga gatatatgtt gcatcacgcc cgtaatcgat cgatggatcg
     3061 ggatgtggtc atcccggcct tactttggcg cggggggaag caaggaataa agggttttgc
     3121 gcatatctgt gctgtcccat ttgttccgtt cggtgcctat ttccagccgg gccaagttcc
     3181 cttcttgatc gatcacggcc gtagcgaggt tgtgggcgat ctcggtgtct ccgggctggt
     3241 aaaagagccc gagtggaaca ccaatcttgg ccagctcggc gtgcgaggca accgcgtagg
     3301 tccaaagggg cggcgtagcg ccgcgaatct gggcatgctt gcgcaatact tccggtgtat
     3361 cgtgctctgg gtcaaacgag agcgaaatga ggcgaatctg ccgggcgcgc tcgggatata
     3421 aggcgatccc ttgggccagc tcggcaaact ttttgtccat gagtggacaa aagtcgggaa
     3481 gagggcatcg tgtataaata aacgtgagtg cgacgacgtt gccgcgcagg tcggagagct
     3541 tcaccgcctg tccgtcctga ttggtcatgg tgaagtcagg agccggttct cccaccgcca
     3601 gcagccggtg ttgtgggccc acgagggcct tgttcttgga tatatttggg gccgcttgca
     3661 cgctcggtgc gggctttaac acctgcaggt ccgaaagctc gtagttacga accgcaccaa
     3721 gcgcacgctc gacccggagc gtcccttcca caaggtcgcc gggtcgcagc gttgccagtg
     3781 tgttcttgtt tttataagcg aatcgcatcc tcattgcatc catgaagccg gggattgcct
     3841 cgtgggcaat ggtcacgtgc tcgagctcgg attcgacccg tttgactact cccttcaacg
     3901 tgtagtcggt catcaccgga tcttctcggg ccggattgga gggtgcggcg agcgaagctg
     3961 gcgcggcttg ttcgcctgat cgatcgtgcc cacaacttgt caataaagcg agtggggaca
     4021 atatcatcac gcgaagtcgt ttacggaaca tgatttgatc atttccatta attagatttg
     4081 cttgtcttct ggtccggtcg atggggccaa ggtgttttgg aagggaaagg tgtcgtccag
     4141 agggctctgg gccagctcgg tcaagaatcg gcccagccgc tcggcgacta ccgtggtgaa
     4201 ctgctctccc ttggccgccg tggcggcccg agggttgccg gcgcccgagt tggtggtcag
     4261 gaggtgccag gggcgggtga tttcgaccca gccgcgattg accgcctcga aacggctggg
     4321 tttcaccgca ccagagtccg cctgctcaag cgcgaccaat cggggaaagt gagcaaggcc
     4381 catgctcgtt tcc
//
LOCUS       DATAQL010001180         5437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1037580, whole genome shotgun sequence.
ACCESSION   DATAQL010001180 DATAQL010000000
VERSION     DATAQL010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037580"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..3268)
                     /locus_tag="VHS97_20350"
     CDS             complement(32..3268)
                     /locus_tag="VHS97_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450618.1"
                     /translation="MFVARRLIEVIVSAGMLVGIFVVSSSSEQATPVPPDTLFDGFET
                     AQPSWQREYTDAAIDLRVHDRSQRAAHGGRLSEHFEFETADGSQFFVSCATPRVPVND
                     ELSVSLFVRANRGGVQLFARVVLPSDIDPETKAPSFVMVPGTVFDQVDRWQKLEVVHM
                     APSIERLARVLRASSRRPVRLDGAYVEKVVVNLLGAPGQSEVFLDDLQISPVPQEELT
                     AWSKSLASGKSAAVNKAGGVAGKRAGTAQGRIRLDRNLLEKRGDDGRWSPWLPTAIDA
                     PGANILRLRQAGFDILVDGDQSDPERLRPAVDREVLLMKRLSGGTSSDSPSRIVDQMA
                     AYPLRQSVAFWQIGEHLGRQRELKARVEELSRFRDTLAAIRGKNDDASRLVTATVDVE
                     LALYARAPTGLDILGIEPRMWGAVQTLTDTYSYLTQRRLLTVRSNLGALFWAWIPAMT
                     PPEVIRNIWGDDTPPSWGTPPIQPEQLRLMTYLALTAGYRGIGYRADADLTRPEGPGR
                     ALWIEMSFLNLEIDLCEQIMAENDRSISMYSLFDPEPVAVPTNATQLGKRTTALPELK
                     PRGDLRGAAIPLRDRKGALLLVADYYENAQFQPSQLAADKVTITPVLPEGAQAFEISP
                     GDVKVLTPERVPGGTRLTLEEFDTTSLILCTGDMGLYERIRVLIDGVRHKAVPLAIEQ
                     AEYMLQAVTEINGRLAADGHQFQSKVVLKLRRQAGFEGPPPDVPDLLAESQRRIKNAR
                     DAWERQDYSVAWAEARRAKRPLRTVMIGHWSQAVAAFARAAESINPEGAKEEDEEPKK
                     VEKTAKKITNPPLLIPPTACPPLISFYTLPEHYIWVDWIKGKPGYRFGRNRVPSGTFD
                     DRDTITASGWVNVSYQMEGIIAEISTVARSEANGPALGPNRPGLDQIAADNRPTNRVV
                     KLKVTAERPEELDSLLPKFLDFPVAAIRSPPIRVEANNLIRISVLVRRPYASPVGTGG
                     IFVRDSIGGEQFQYRSNAPLGSFNRVVLFRKAPADGTFTVTLGLAGYAEAFFDDFRVE
                     VIEHEGTTGDSDVVQRRPRGRNASAPERPDPTLPAAAARSIDSRPRPR"
     gene            3731..4150
                     /locus_tag="VHS97_20355"
     CDS             3731..4150
                     /locus_tag="VHS97_20355"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX3450619.1"
                     /translation="MARPPTETLTSREVQVMDAVWRLGEATAELIREALPEPLHDSTV
                     RTLLRVLEFKGYLSHEVRGKAYVYRAAVERKKAQGHALRSVLMRFFGGSAEDLVLRLI
                     EDERITPEQLDALRRTEPMRRGRGASRRRTGGKATGE"
     gene            4153..>5437
                     /locus_tag="VHS97_20360"
     CDS             4153..>5437
                     /locus_tag="VHS97_20360"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3450620.1"
                     /translation="MIDLLGSTFLGSRSALIALDLALKATALMTFTFACHGALGRRRA
                     LARSALWNACLIGLLLLPAACLAFPRLHVIVAPTRSMRRYETAAPSRDVPPAAKSAAD
                     DVLSVSQSSMDRDTMGSIGSDAVRQPASGRPYLRSEDQPAPRLGGTGIAVTVYLAMAT
                     LLLLRLAASVTAVARLRRQCVVVEDEQWVGALDFWRTRLGITRRVPLLASERVSVPIV
                     AGWLCPAIIIPKAMAAKANSPLIGAVLVHELAHVRRADYGWNLVRKLVQIVYWPNPLV
                     WLVGWVVGSVREQACDDLCVYGLGGAAAYRNSLIEVASGLVRLPDPALGMAMARATNL
                     GRRLAWIDRTRGAPRCLSRWPARLALGLAMPAVAGVLGAVELARATATANPGNTPARS
                     AEMPQENPRPDQPSWIEVTVRAKDSGRPLEGATVRP"
BASE COUNT          982 a         1684 c         1721 g         1050 t
ORIGIN      
        1 gccacgagtc tctgccgaga gtttctcagg ttcaccgcgg gcgcgggcgc gagtcgatgg
       61 accgcgccgc cgcggccggc agggtcggat cgggacgttc gggggctgac gcattccgtc
      121 ctcgcgggcg ccgctgcacg acatccgaat caccggtcgt tccttcgtgc tcgatcactt
      181 cgacccggaa gtcatcgaaa aaagcttcgg cgtaacccgc gagtcccaag gtgacggtga
      241 atgtcccatc cgcgggtgcc ttgcgaaaaa gcaccactcg gttaaacgag cctagagggg
      301 cgttcgatct gtactgaaac tgctcaccgc ctatcgagtc gcgcacgaaa atgccacccg
      361 ttcccactgg gctcgcgtag ggacgcctca caagtaccga gatccggatc agattgttgg
      421 cctcgactcg tatcggtgga gagcgaatcg ctgccaccgg gaaatcgaga aacttgggga
      481 gcagagagtc cagttcctcg ggtcgttcgg cggtcacttt caatttgacg actcggttag
      541 ttggtcggtt gtctgccgca atctggtcca gaccggggcg gttgggcccc aaagccgggc
      601 cgttggcctc cgaacgggcc acggtggaaa tctcggcgat gattccttcc atctgatagc
      661 tgacgttgac ccagcctgac gccgtgatgg tgtcacgatc atcgaaggtt ccagacggta
      721 ctcggttgcg gccaaatcgg taacccggct tgcccttgat ccagtcgacc cagatgtagt
      781 gttccgggag cgtgtagaac gagatgagcg gcggacatgc ggtaggtggg atcaagagcg
      841 gcgggttggt gatcttcttc gcggtctttt cgactttttt gggttcctca tcctcttcct
      901 tggcgccctc gggattgatg ctctcagcag ccctggcaaa ggcggccacg gcctgcgacc
      961 aatgaccgat catcacggta cggagaggtc gcttggcccg acgggcttcg gcccaggcaa
     1021 ccgaatagtc ctgccgttcc cacgcatcgc gggcgttctt gatccgtctc tgcgactcgg
     1081 ccaagaggtc ggggacatca ggagggggac cctcaaagcc agcctgacgg cgcagcttca
     1141 agacaacctt gctctggaac tggtggccgt cggccgccag acgaccgttg atctcggtaa
     1201 ccgcctgaag catgtactcc gcctgctcga tcgccagagg gaccgctttg tggcggaccc
     1261 cgtcgatgag cactctgata cgttcatata aacccatgtc gccagtgcaa aggatcaggc
     1321 tggtcgtgtc gaactcttcc agcgtgagcc ttgtgccgcc aggcacgcgc tcgggtgtca
     1381 gaacctttac gtcgccgggg ctgatctcga aggcctgagc cccctctgga agaaccggtg
     1441 tgatggtgac cttatcggca gccaactgcg acggttgaaa ctgcgcgttc tcgtaataat
     1501 ccgccaccag caagagcgct cccttgcgat cgcgaagcgg tatggcggcg ccgcgcaaat
     1561 cgccccgagg tttgagctcg ggcagcgccg tcgtcctttt ccccagctgg gtcgcgttgg
     1621 ttgggaccgc cacaggttcg ggatcgaaaa ggctatacat ggaaatcgac ctgtcgtttt
     1681 cggccataat ctgctcgcag aggtcgatct caagattgag gaagctcatc tcgatccaga
     1741 gggcgcggcc gggaccctca ggtcgggtca agtcggcgtc ggcgcgatat ccgatcccgc
     1801 ggtagccggc cgtgagggcg agataagtca tgaggcgaag ttgctcgggc tgaattggcg
     1861 gcgttcccca cgatggagga gtgtcgtcac cccaaatatt gcgaatcacc tcgggaggag
     1921 tcatggccgg aatccaggcc cagaataagg cgcccaggtt cgatcggacg gtcagcaacc
     1981 tccgctgagt aagataggaa taggtatcgg tcaaggtttg aactgctccc cacatcctgg
     2041 gctcgatacc caaaatgtcc aggcccgttg gtgcgcgggc ataaagcgcc agctctacgt
     2101 ccacggtcgc cgtgaccagg cgcgaagcat cgtcgttctt gcctctgatg gccgccagag
     2161 tgtcgcgaaa ccgcgacagt tcttccacgc gtgccttgag ctcacgttgt ctacccagat
     2221 gctcgccgat ctgccagaac gcaactgact gccgcaacgg atacgccgcc atctgatcga
     2281 cgatgcggct cggactgtca ctcgatgtcc cgccgctcag gcgcttcatc agcaagacct
     2341 cgcggtcgac ggccggccgc agccgctcgg gatcggattg atcaccatcg accaaaatgt
     2401 cgaatcccgc ctggcgcagc ctgaggatgt ttgctcccgg agcgtctatt gccgtgggta
     2461 gccagggcga ccatcggccg tcgtctcccc gcttttcgag gaggttgcga tccagacgaa
     2521 tgcgaccttg ggccgtgccg gctcgtttcc ctgccacgcc gccggcctta ttgacggcag
     2581 cggactttcc cgaggcaaga gactttgacc aggctgtcaa ctcttcctgc ggcacaggac
     2641 tgatctggag gtcgtcaagg aacacttccg attgacccgg agcacccaac aggttgacta
     2701 ccaccttctc gacgtacgcg ccgtccagtc ggaccgggcg ccgcgacgag gccctgagca
     2761 cacgagccag acgctcgatt gagggcgcca tgtgcacgac ttcgagcttt tgccagcggt
     2821 cgacctggtc gaagaccgta cccgggacca tcacgaacga aggggccttg gtttccgggt
     2881 cgatgtccga cggcaggacc acccgcgcaa acagctggac ccctccgcgg ttggctcgaa
     2941 cgaacaggct cacactcaat tcgtcgttga ccgggacccg cggcgtggca caacttacga
     3001 aaaattggct gccgtcggcg gtctcgaatt caaagtgctc ggagagccgt ccgccatggg
     3061 ccgctcgctg cgagcggtca tgcacccgca ggtcgatcgc cgcgtccgtg tattcgcgct
     3121 gccagcttgg ctgggcagtc tcaaacccgt cgaatagcgt atccggcggt accggcgtcg
     3181 cttgctcgct actcgacgag acgacgaaga ttccaactag cattccagcg gaaacgataa
     3241 cttcgatgag tcggcgtgcc acgaacatcg gctcgagacc tccctgtccg cgaacctact
     3301 ggaattgatg cgcgatcgat cgagaagcgt gatcgtaacg tccttgccga tatcggggaa
     3361 ggtcagtcgc gccgcgccct tgtccttccg tccttcaggt gcaatatcgg aaactggcgt
     3421 gaccgccggc agattgccat ggcacgattc gaaccgactc gagcacgaac ggattctgcc
     3481 ttgagggagc gtcacgagac gagtggaact ggaggtgcga tcatgggccg ttcgtgcatc
     3541 ggcgcagtcg tggctgtttc gtctctgtgc gggctgttca ctgacgaggc cttcgtacac
     3601 ccggcttccg gcatcgtcgt gaatgcgcgg ggggaagtgt tatgaaactc acaggaagtg
     3661 cttgacgtag ctttgtcgcg atgggtatcc tgcctttacc tgttagataa ctaacggagg
     3721 ataactggta atggcgcgac caccaacgga gacgttgacc agccgggaag ttcaggtcat
     3781 ggacgccgtc tggaggctgg gcgaagcgac cgcggagctc attcgcgaag cgttgcccga
     3841 gccgctacac gactcgacgg ttcggacact cctacgcgtg ctcgagttca agggttacct
     3901 cagccacgag gtccgaggga aggcgtacgt ctaccgcgcc gcggtcgagc ggaaaaaggc
     3961 tcagggtcac gctctgcgaa gcgtgctcat gcgatttttt ggcgggtcgg ccgaggatct
     4021 ggtcctacgg ctgatcgagg atgaacggat cactcccgag cagcttgacg cgttgcgacg
     4081 cacggaaccc atgcgacggg ggcggggcgc atcccggcgc aggaccggcg gcaaagcaac
     4141 aggagaatga tcatgatcga cctcctcggt tcgacgttcc ttggatcgcg atcggccttg
     4201 atcgccctcg acctagccct caaggctacg gcgctcatga cctttacttt tgcatgtcac
     4261 ggcgcgctgg gccgccggcg cgcattggct cgttccgcgc tttggaacgc ctgcttgatc
     4321 ggcctgctgc tattgcccgc ggcctgcctg gctttcccgc ggctgcacgt catcgtcgca
     4381 ccaacgcggt ccatgcgacg gtatgaaaca gcggcaccaa gccgtgatgt gccaccagcc
     4441 gcgaaatccg ccgctgacga cgtactttcc gtctcacaga gctcgatgga tcgggacacg
     4501 atgggttcaa ttggatcaga cgcggtgcgg caaccggcat ctgggcgtcc ttacttacgg
     4561 tcggaagacc agccggcgcc gcggctcggt ggcaccggaa tcgccgtcac cgtgtatctg
     4621 gcgatggcga cgctgctctt gctgcggctg gccgcctcgg tgacggcggt cgcgcggctg
     4681 cggcgccaat gtgtggtcgt cgaagacgag cagtgggtgg gcgccttgga cttttggcgc
     4741 actcggttgg gaatcactcg gcgagtgccg ttactggctt cggagcgggt ctcggtccct
     4801 attgtcgccg gctggctttg tccggcgatc ataattccaa aggccatggc agcaaaggcg
     4861 aattccccgc ttatcggcgc cgtacttgtg cacgagctgg cacacgtccg ccgcgcagac
     4921 tacgggtgga acctggtgcg aaaactagtg cagatcgtgt actggccgaa cccgctcgtg
     4981 tggttggtcg gctgggtcgt cggatccgtc cgcgagcagg catgtgatga cttgtgtgtg
     5041 tacggcctgg gcggcgctgc cgcctatcgc aattcgttga tcgaggtcgc ctcgggacta
     5101 gttcgcctgc ctgacccggc cctgggaatg gccatggcgc gagccacaaa cctcggccgc
     5161 cgactggcgt ggatcgaccg aacccgcggc gctcctcgct gcctgtcgcg ctggccggcc
     5221 cgcctcgccc tcgggctggc aatgccggcc gtggccggtg tgctcggcgc ggtcgaattg
     5281 gcccgcgcga ccgcgaccgc gaacccaggc aacacgccgg cacgctccgc cgaaatgccg
     5341 caggagaacc cccggccgga tcagccgtcc tggatcgagg tcaccgtccg cgccaaggac
     5401 agcgggcggc cgctggaagg cgcgaccgtt cggccct
//
LOCUS       DATAQL010001181         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1495089, whole genome shotgun sequence.
ACCESSION   DATAQL010001181 DATAQL010000000
VERSION     DATAQL010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495089"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1378
                     /locus_tag="VHS97_20365"
     CDS             50..1378
                     /locus_tag="VHS97_20365"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3450621.1"
                     /translation="MLENAPTNVLLADRDLKIVYVNPASLATLRKLERHLPVKPENAL
                     GSSIDLFHKDPAYQRKILSNDKNLPVRANIKIGPETADLLVTAIYDKNKQYIGPMVTW
                     ELITEKLDNERKIQEASERERVQAVELRTKVDGILDVVNSASRGDLTREIQVKGADSI
                     GQMGEGLAKFLANLRQSVSSISEMAQTLASSSRQLTAVSQQMASNAEETAAQANVASA
                     AAEQVSKNVTTVATGADEMGASIREIAKNTNEAARVATAAVKVAEKTNDTVAKLGESS
                     AEIGNVIKVITSIAQQTNLLALNATIEAARAGEAGKGFAVVANEVKELAKQTAKATED
                     ISRKIEAIQSDTKGAVDAIGQIGKIINQINDIQNAIASAIEEQTATTGEISRNVAEAA
                     KGSNEIAQNITGVAQAARSTTEGASNTKDSADGLSKMALELQQLVGQFKY"
     gene            1413..2978
                     /locus_tag="VHS97_20370"
     CDS             1413..2978
                     /locus_tag="VHS97_20370"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX3450622.1"
                     /translation="MTRADYVMMEQSPVSTPIKRLWRVGLLATDLAGVRLGRTGKGAD
                     DARRRLVQRLGSLRGLPQKIGQLLALAGPDGPSAFAPLTETRSALPLSAALDVMETSL
                     GRPWTDCFHSIDAKGIAASLGQVHRAVLLDGRPAAVKIQYRDSAANVDADLGALDWLS
                     LPFGGLRGGFDLAAYRRELGGMLRQELDYRREAQSLRRFRALAAGCEAVVVPEVIDEL
                     STDRILTMTWLEGEPFEAVRTWPERDRRQVAYALLRLFLTSGFAWRFLHADPHPGNYR
                     FLRDGNGVRVGILDFGCIVPLDEDHAQALFRLVEGASSSSVDHVLADYRSLGFNPDLL
                     EPIAHLLPALNQVMFEPFRTEGLFSLSDWRLSERVENLLGSFRWNFRFAGPAGLIFVV
                     RAYLGLVRYMQALRVDVNWMEVWRTSVGQGRAPAGPLAGKPAAAGGREVLSRHLRVAV
                     WEAGQVRVDLTFKAVLAESLPDLIPDELGEKLRARGLDVQRIADHAAGSLFAPGELFQ
                     LAEESKLVRVWLE"
     gene            3021..3392
                     /locus_tag="VHS97_20375"
     CDS             3021..3392
                     /locus_tag="VHS97_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450623.1"
                     /translation="MRALLIDDSRAVRVIIRNILLEMGMEVVEAGNGREALEQLRQNP
                     DVELVLVDWNMPVMNGLDFIRVVRGQHSYDGVRIVMVTTETESEQVCRALNAGANEYV
                     MKPFNREVLVAKLNMLDVFGE"
     gene            3396..>3898
                     /locus_tag="VHS97_20380"
     CDS             3396..>3898
                     /locus_tag="VHS97_20380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450624.1"
                     /translation="MAKIRILVVDDSVVFRRALSDEFSRDPALEVVGTASNGRIALAK
                     LGQLSPDLVILDIEMPEMDGLQALKELRKTYPNLAVIMFSSLTERGAAATLDALALGA
                     TDYFTKPAEMWGLDESLRVIREQMIPMIKAICAPPRPLTVPAGASPTGLTGPKAPVAT
                     GTVRVLA"
BASE COUNT          737 a         1279 c         1257 g          625 t
ORIGIN      
        1 aattacggac aagctggaga tcgaaaacga gcaatcacgc ctggcgtcga tgctggagaa
       61 cgcgcccacg aacgtgctct tggccgaccg cgacctgaag atcgtctacg tcaatcccgc
      121 ctcgctggcc actctgcgca agctggagcg gcacctgccc gtcaagccag agaacgcgct
      181 gggctcgtcg atcgacctct tccacaagga cccggcctac cagcgcaaga tcctgtccaa
      241 cgacaagaac ctgccggtgc gggctaatat caagatcggt cccgagactg cggacctgct
      301 cgtaactgcc atatacgaca agaacaagca atacataggg ccgatggtca cctgggaact
      361 gatcacagag aaactggata acgagcgcaa gatccaggaa gcatcggagc gcgagcgcgt
      421 gcaggcggta gagctgcgga ccaaggtgga cggcatcctt gatgtggtca attcggccag
      481 tcgcggcgac ctcacgcgcg agatccaggt caagggcgcc gactcgatcg gccagatggg
      541 cgagggactg gcgaagttct tggcaaactt gcgccagagc gtctcgagca tttcggaaat
      601 ggctcagact ctcgccagct cgtcgcggca actgaccgcg gtgagccagc aaatggcgtc
      661 caacgccgag gagaccgcgg cgcaggccaa cgtcgcctcc gcggcggccg agcaggtgag
      721 caagaacgtc acgacggtag ccaccggcgc cgacgagatg ggagccagca tccgtgagat
      781 cgccaagaat accaacgagg ccgccagggt ggccaccgcc gccgtcaagg tcgccgagaa
      841 gactaacgac accgtggcca agctcggcga gtcgagcgcc gagatcggca acgtcatcaa
      901 ggtcatcacc agcattgccc agcagaccaa cttgctggcc ttaaacgcta ctatcgaggc
      961 tgcccgcgct ggcgaggcgg gcaagggctt cgccgtcgtc gccaatgagg tcaaggaact
     1021 ggcgaagcag accgccaagg ccacggagga catcagccgc aagatcgagg caatccagtc
     1081 cgacaccaag ggcgcggtcg atgccatcgg gcagatcggc aagatcatca accaaatcaa
     1141 cgacattcag aacgcgatcg ccagcgccat cgaggagcag acggccacca ccggcgagat
     1201 cagccggaac gtggccgagg cggccaaggg aagcaacgag atcgcccaga acatcaccgg
     1261 cgtggcccaa gccgcccgca gcactaccga gggtgcgagt aacaccaagg actcggccga
     1321 tgggctctcc aagatggctc tggagctcca gcaactggtc ggccagttca agtattgaat
     1381 gttggagcac cgcctccccg aggactgccg cggtgacccg cgcggactac gtcatgatgg
     1441 aacaatcgcc tgtgtccacg ccgatcaagc gcctctggcg cgtgggctta ctcgccacgg
     1501 acttagccgg cgtgcgccta gggcgcacgg gcaaaggcgc ggacgacgcc cggcgccggc
     1561 tggtccagcg ccttggctcg ctgcgcggcc tgcctcagaa aatcggccag ctccttgccc
     1621 tcgccgggcc ggacggcccc tcagccttcg cgcccctcac cgagacacgc tcagccctgc
     1681 cgctgagcgc ggcgctcgat gtgatggaaa cctcgctcgg ccgcccgtgg actgattgct
     1741 tccactcgat cgatgccaaa ggcatcgccg cctccctggg acaggtccac cgcgccgtcc
     1801 tcctggacgg gcgtccggcc gccgtcaaaa ttcagtaccg tgacagtgcg gccaacgtcg
     1861 acgccgacct cggtgccctc gactggctct cgctcccctt tggcggactg cgcggcgggt
     1921 tcgacctggc ggcctaccgg cgcgagctcg gcggtatgct gcgacaggag ctcgactacc
     1981 gccgggaggc ccagtccctc cggcgattcc gcgcactcgc cgcgggctgc gaggcggtag
     2041 tcgtccccga ggtcatcgac gagctctcca ccgaccgcat tctcaccatg acctggttgg
     2101 aaggggagcc gttcgaagca gtgcgaacct ggcccgagcg ggaccggcgg caggtggcct
     2161 acgctctgct gcggttgttc ctcacaagcg gtttcgcctg gcgcttcctg cacgccgacc
     2221 cgcaccctgg caactaccgc ttcctccgcg acggcaacgg cgtccgcgtg ggcatcctcg
     2281 atttcggctg tatcgtgccg ctggacgagg accacgcgca ggcactcttc cgattggtag
     2341 agggggcttc gtcgtcgtca gtcgatcacg tcctggcgga ttatcggtcg ctcggattta
     2401 accccgacct gctggagccg atcgcccatt tgctccccgc gctcaaccag gtcatgttcg
     2461 agccgttccg taccgaggga cttttctcgc tctcggactg gcgcctcagc gagcgggtcg
     2521 aaaatcttct cggttcgttc cgctggaact tccggtttgc cggaccggcc ggcctgatct
     2581 tcgtcgtccg ggcatacctg ggtctagtcc gctacatgca agccctgaga gtggacgtca
     2641 actggatgga ggtctggcgc acctcggtcg gccaggggcg agcgccggcc ggcccgcttg
     2701 cgggcaagcc ggcagctgcc ggcggccgtg aggtgttgtc gcgccacttg cgagtggccg
     2761 tctgggaggc gggccaggtc cgcgttgacc tgaccttcaa agccgttctc gctgagagcc
     2821 tgcccgactt gatacccgac gagctgggag agaagctgcg ggcgcgcggg ctggacgtcc
     2881 agcggatcgc cgatcatgcc gccgggtccc tgttcgcgcc gggggagttg tttcagctgg
     2941 ccgaagagag caaactggta cgagtctggc tggagtgaac gccgccggca ctcggatgtc
     3001 ccaccgccac ggagacgcat atgcgtgcgc tgctgatcga cgactcgcgg gccgtccggg
     3061 tcatcatccg caacatcctg ctcgagatgg ggatggaggt tgtcgaggcc ggcaacggcc
     3121 gcgaggcact agagcagttg cggcaaaacc ccgacgtcga gctcgtgctg gttgattgga
     3181 acatgccggt gatgaacggg ctcgacttca ttcgcgtggt ccgcggccag cactcgtacg
     3241 acggggtgcg catcgtcatg gtgacgacgg aaaccgagag cgagcaagtc tgccgcgcgc
     3301 tcaatgccgg cgcgaacgag tacgtcatga agccgttcaa cagggaagtc ctggtcgcga
     3361 agctcaacat gcttgacgtg ttcggggagt agcgcatggc gaagattcga atcctcgtcg
     3421 tcgatgattc cgtcgtcttc cgccgcgcgt tgtccgacga attctcgcgc gaccccgcgc
     3481 tggaagtggt cggcaccgcc tccaacggcc gaatcgccct ggccaagctg ggtcagttga
     3541 gcccggacct ggtcatcctc gacatcgaaa tgccggagat ggacggcctg caggcgctca
     3601 aggagctgcg caagacgtac ccaaacctag cggtaatcat gttcagctcc ctgacggagc
     3661 gcggggctgc ggcgactctc gacgccctgg cgcttggggc gactgactat ttcaccaagc
     3721 ccgctgaaat gtggggcctg gacgaatcgt tgcgggtcat ccgggagcag atgatcccca
     3781 tgatcaaggc catttgcgcc cccccgcgcc cgctgacggt accggcaggc gcaagtccga
     3841 cgggcctgac cgggccaaag gcgccagtcg cgaccggcac ggtccgagta ctggccat
//
LOCUS       DATAQL010001182         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3173497, whole genome shotgun sequence.
ACCESSION   DATAQL010001182 DATAQL010000000
VERSION     DATAQL010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173497"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHS97_20385"
     CDS             <1..318
                     /locus_tag="VHS97_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450625.1"
                     /translation="GETDLRGVFVAEGLRGLVTAVARKANSQYSFYRGKTYLGQAAAP
                     PAVNQAPAQSGNANGAIMNQSLDANLKEQNFSNSVRQVERLQQRYNPPAGNPKGAPAG
                     GFR"
     gene            429..869
                     /locus_tag="VHS97_20390"
     CDS             429..869
                     /locus_tag="VHS97_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX3450626.1"
                     /translation="MTRAFMMALLLLIWLAPVFAGEKPAAGGSQDASSAHRVFELRTY
                     YTNEGKLTDLHKRFRDHTCRLLKKHGAELIGFWTPLDEKDGKGSKLIYLVAFPSREAG
                     KKTWEEFGKDPEWRKVYEESHKNGVLNKKVDSVFMEPTDYSEMK"
     gene            complement(1095..2249)
                     /locus_tag="VHS97_20395"
     CDS             complement(1095..2249)
                     /locus_tag="VHS97_20395"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX3450627.1"
                     /translation="MLEQASSVEEREQAAIEPEIAIGRPEERGDRAVIARLIRRLRIS
                     DSPKDFQTVATTVLRTSLELSAVAWIPKHPHDSAVISGSVAGLNAAAFRAFPSPAGRE
                     SRLVYNFDADSSQQSPSAAVRRYASVAAGSLGWLLAINPLDDHPFTEADLERLQYVAS
                     LIAAQLSNARIYADLKELLFGVIRGLTAAVDAKDPYTFGHSERVARIAARLGEQLGMS
                     AAARSDLYLAGLLHDVGKIGIDDQVLKKTGPLTPEEYRNIQSHVEIGVTILKDLKKLS
                     HILPGVKHHHESVDGTGYPDHLCGSDIPLEARILAVADSFDAMSSSRPYRKRLSFAQI
                     DKILLDGRGIQWDADVIDALFACRPDIEAIRQKGLGESLIGAVDVTLGRT"
BASE COUNT          601 a          768 c          810 g          526 t
ORIGIN      
        1 ggcgagacag acctacgggg ggtgtttgtt gccgaaggcc tacgagggct ggtcaccgcg
       61 gtcgcccgca aggcgaattc gcagtattcg ttttatcgcg gcaagactta tttgggacaa
      121 gctgccgcgc ccccggcggt caatcaagca ccagcgcaaa gcggcaatgc gaatggcgcg
      181 atcatgaacc agtcattgga cgccaatctc aaggagcaga atttctcgaa tagcgtccgc
      241 caggtcgagc gactgcaaca gcggtacaat ccgcccgcgg gcaaccccaa gggagcgccg
      301 gccggtggtt tccgctagcc tcttggaggc agccgcagcg aaaggctata aacgtgagtg
      361 cgaccgacgg cgaaaggctg ccggcgtgag caatgaaaac gccccattga ccaaggagaa
      421 cagatccgat gactcgcgcg ttcatgatgg ccctcttgct tctcatctgg ctggcaccgg
      481 ttttcgcggg cgagaagccc gcggcaggtg ggagccagga tgcctcgtcg gctcatcgcg
      541 tattcgagct tcgaacgtat tacacgaacg agggcaaact gaccgacttg cacaaacgtt
      601 ttcgcgacca cacctgccgg ctcttgaaaa agcacggagc cgagctgatc ggattctgga
      661 ccccgctcga cgaaaaggac ggcaagggga gcaagcttat ctacctggtc gcattcccca
      721 gtcgcgaggc cggcaaaaag acttgggaag aattcggaaa ggacccggaa tggcgtaagg
      781 tttacgaaga gagccacaag aacggcgttc tcaacaaaaa ggtcgattcg gtctttatgg
      841 agccgaccga ctatagcgag atgaaatgac tgcggcggga cgccaagcag attgcagctg
      901 tgattaagag taagagtatg atgaggattt agagcaagag catgattaag agtaagacgc
      961 agaccacgac gttgcctttc tggtcctaat cctaatctta atcgtgctcc taatcatctt
     1021 cataatctta atcttcatcc taatcttact cctcttcata tgcgttagcg caccaaacga
     1081 aaccgcacgg acgatcaggt tctaccaagc gtcacgtcga ccgcgccaat caagctctca
     1141 cccagacctt tctggcggat tgcctcgatg tcgggtcggc aggcgaagag cgcgtcaata
     1201 acgtcagcgt cccactggat accgcgtcca tccaagagga tcttatcgat ctgggcgaag
     1261 ctgaggcgct tgcggtaggg gcggctgctc gacatggcgt cgaacgagtc ggcgaccgcc
     1321 aagattcgtg cctcgagcgg gatatcgctg ccgcagagat ggtcggggta tccggtccca
     1381 tcgacgctct cgtggtggtg cttgacgccg gggaggatgt ggctgagctt tttcaagtcc
     1441 ttgagaatcg tcacgccgat ttcgacatgc gactgaatgt ttcgatattc ttctggtgtc
     1501 aaagggccgg ttttcttgag aacctggtcg tcgatgccga tcttgccgac gtcatgcaag
     1561 aggcctgcga ggtaaaggtc gctgcgcgcc gcggcggaca tgcccagttg ctcgcccagt
     1621 cgcgccgcga tgcgggcgac ccgttcggaa tgcccgaacg tgtaaggatc tttggcgtcg
     1681 actgccgctg tgaggccgcg gatgactcca aaaagcaact ccttaagatc ggcatagatt
     1741 cgagcgttgg aaagctgggc ggcgatcagc gatgcgacgt attgcaatcg ctcgaggtcg
     1801 gcctcggtga acggatggtc gtcgagcggg ttgattgcca aaagccagcc gagcgagccc
     1861 gcggccaccg acgcatagcg ccgtacggcg gctgagggac tttgctggct gctgtcggcg
     1921 tcgaaattgt acacgagccg cgattcgcgg ccggcaggcg acggaaacgc acgaaaggcc
     1981 gctgcattta gacccgcgac agagccgctg atgaccgccg aatcatgcgg gtgctttgga
     2041 atccacgcca ctgccgacag ctcaagcgag gtcctcagta cggtggtggc aaccgtctga
     2101 aagtctttgg gggagtccga aatccgcaat cgccggatca ggcgcgcaat cacggcccga
     2161 tcgccgcgtt cctccggccg cccgatagct atttcgggtt cgatcgctgc ctgttctcgc
     2221 tcttccacac tgctggcttg ctcgagcatc gcgctttcct cctactttgt tcccacaagt
     2281 agtctaggac acaatcttgc gcggattgcc tacctgaggc ggaatgttcc ttccgcgcag
     2341 ccacacgtcc ggcatgttcg agtcatttac tggacagagg gaggtccccg gcatagagaa
     2401 cagaagaggc ccgtctgccg agttcgagcg atctgatggg aaaacaatct gagacaggca
     2461 ggggctcaac ggttagtctc cgaaccccgc acgttcggcg cgtattcaaa agccacaagc
     2521 caaaagccac aaacgtctta gcacccatga ccgaatctat ccgcaattct ggaataaacc
     2581 tcgactcgac gcgagaggtt ttggccgatt ggagcatcca cggcgatggc tcgagcgact
     2641 cgaccatcac gcccggtgtc ggcgccatcg gttcgggaga gcgaaacggc cctgactcgg
     2701 aaggg
//
LOCUS       DATAQL010001183         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2562328, whole genome shotgun sequence.
ACCESSION   DATAQL010001183 DATAQL010000000
VERSION     DATAQL010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2562328"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VHS97_20400"
     CDS             <1..936
                     /locus_tag="VHS97_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3450628.1"
                     /translation="TGYYADNVVIPIDQAAEAYSRDTGVELVLPATDELLDLLPPDGG
                     SYEAACELFAWIEQLDLISRDELTIPTSDVRLLVPIANPPKMLFLAGNYAKHVAERGG
                     SAAERAETFPYVFMKPPSTTLTHPGDPVLIPRVSPDQIDWECELAIVIGRRCRHVDED
                     EAMGCIAGYTIINDISDRSFKPNPKRKPRERDKFFDWLHGKWHDSFCPMGPCILSADV
                     VPDPQAIPLKLTLNGHIKQDASTAEMIFPVPAIVSFLSEFVTLQPGDLIATGTPSGVG
                     SASGTFLRPGDYMRATIGPIGTLETPVEAEDEDDL"
     gene            1026..>1689
                     /locus_tag="VHS97_20405"
     CDS             1026..>1689
                     /locus_tag="VHS97_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX3450629.1"
                     /translation="MTSFRKLMAANRGEIAIRIFRSAHELGIRTVAIYSHEDRFAIHR
                     LKADEAYEVGKPGEPIRTYLNIDAIVELAKAKDVDAIHPGYGFLSENAEFARACQRAG
                     IVFVGPRPELLDLLGDKVAARKLARDAGVPVLSGSDGPVMPGKEAHALAESLGYPVIV
                     KASMGGGGRGMRVVEAADGLDAALDQARREAGAAFGVPDVFLEKFIKRARHLEVQVLG
                     DRH"
BASE COUNT          304 a          536 c          531 g          318 t
ORIGIN      
        1 acaggctatt acgccgacaa cgtggtgatc ccaatcgacc aggcggccga ggcctacagc
       61 cgcgataccg gggtggagct ggtgctgccg gcgaccgacg agctgctcga cctcttgccc
      121 cccgacggcg gctcttatga agccgcctgc gagctcttcg cctggatcga acagcttgat
      181 ctgatctcac gcgacgagct gacgatcccc acctccgatg tccggctctt ggtgccaatc
      241 gccaatccgc ccaagatgct ctttttggcc ggcaattacg ccaagcacgt cgccgagcgc
      301 ggcggatcgg cggccgaacg agcagagacg tttccgtatg tctttatgaa gcctcccagc
      361 acgacgctga cgcaccccgg cgacccggtc ttgatccccc gcgtctcgcc cgaccagatc
      421 gactgggaat gtgagctggc catcgtgatc ggccggcggt gccggcacgt cgatgaagac
      481 gaggcgatgg gatgcattgc cggctacacc atcatcaacg acatcagcga ccgctcgttc
      541 aaacccaacc ccaagcgcaa gccgcgtgag cgcgacaagt ttttcgactg gctgcacggc
      601 aagtggcacg actcgttctg tcctatgggt ccgtgtatcc tttccgccga cgtcgtgccc
      661 gacccgcagg cgattccctt gaagctgacg ttgaacggtc atatcaagca agacgcatcg
      721 actgccgaga tgatcttccc ggtgccggcg atcgtgtcgt tcctgtcgga gtttgtcacg
      781 ctgcagccag gcgacttgat cgccaccggc acaccttcgg gcgtcggctc ggcctcggga
      841 acgtttctcc gacccggcga ctacatgagg gccaccatcg gtccaatcgg tacgctcgag
      901 accccggtcg aagccgagga tgaagacgac ctttgacact agcgcttttg aattgggttt
      961 aacttactgg cccaaggtca attctatctc cctcccccat tgtgagggag ggttggggtg
     1021 gggggatgac ctcgtttcgc aagttgatgg ccgccaaccg cggcgagatc gccattcgca
     1081 tttttcgctc ggcccacgag ctcggcatcc ggaccgtggc gatctactcc cacgaagacc
     1141 ggtttgccat tcatcgcctg aaggccgacg aagcctacga agtgggcaag ccaggcgaac
     1201 cgatacgcac ttatctgaac atcgacgcga tcgttgaatt agccaaggcc aaggacgtcg
     1261 acgcgattca tccgggctac ggattcttgt cggagaatgc cgagttcgcg cgggcgtgcc
     1321 agcgggcggg gatcgtgttt gtcggtccca ggcccgagct cctggatttg ctcggcgaca
     1381 aggttgccgc acgcaagctg gcgcgcgacg cgggcgttcc ggttctgtcg gggagcgatg
     1441 ggccggtcat gcctggcaaa gaggcacatg ctttggccga gtcgcttggc tacccggtga
     1501 tcgtcaaggc gtcgatgggg ggcggcggcc ggggaatgcg cgtcgtggaa gccgccgatg
     1561 gtctcgatgc cgcactcgac caggcgcggc gcgaggccgg cgcggcgttc ggggtgcccg
     1621 acgtcttcct cgagaagttc atcaaacggg ccaggcacct cgaggtccag gtcctcggag
     1681 accggcacg
//
LOCUS       DATAQL010001184         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3938192, whole genome shotgun sequence.
ACCESSION   DATAQL010001184 DATAQL010000000
VERSION     DATAQL010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3938192"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VHS97_20410"
     CDS             <1..1062
                     /locus_tag="VHS97_20410"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3450630.1"
                     /translation="IAAVLIALTTLSNSEPVQENVRPRVARGEHGAQIASFALSPTSA
                     LIATTNYAGRVTLRAPEREWQIERYMDFPGYATAVAFSPDGRYLAAVGNPTGICLWDL
                     VAARNEPTKITLVSIQAPQRIIFSSDGQYIAVTSSLDGTILLWDLATRRDRMVLHQPS
                     AVVSVAFSPDGRWLATAGRVNRSILIWDLQCGSPRSLQEGEPAGVTLAIAFSPDGALL
                     ASAGLPEHHVRLWDLETRRVCREFAGHERSVNSVAFSPDGSLLATAGNDGMLGLWTVT
                     TGERLLSLDGQAGYPRTVAFSPDGRTIALATGNDDDIRLWYLAELTRARPGSNSLPIE
                     LGPIPRQCDRRNLKRDPTP"
     gene            complement(1577..>1957)
                     /locus_tag="VHS97_20415"
     CDS             complement(1577..>1957)
                     /locus_tag="VHS97_20415"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450631.1"
                     /translation="GDLAALGRNLLMQSRWSEAEPILREGLAIREKAVPDDWSRYDAM
                     SQLGGALLGQGRCAEAEPLIVPGYEGVKRREAKIAVPETFRLREAAERAVRLYEDWGK
                     TEQATAWKARLGMPDQPADLFAGP"
BASE COUNT          364 a          645 c          586 g          362 t
ORIGIN      
        1 atcgccgcgg ttctgatcgc tctgaccacg ctttcaaact ccgagcccgt gcaagaaaac
       61 gttcggccgc gcgttgcccg gggcgagcac ggagcccaga tcgcgtcgtt cgcgttatca
      121 ccaacaagtg cgctgatcgc aacgaccaac tacgcgggcc gcgtaacgct tcgggctccg
      181 gagagagagt ggcagatcga gcgatacatg gactttccgg gatacgccac ggcggtggcg
      241 ttctctcccg acggcaggta tctcgccgct gtgggaaatc cgactggtat ttgcctgtgg
      301 gatttggtgg ccgccaggaa tgagccaacc aagatcacat tggtctccat ccaggctccc
      361 cagcgcatca tcttctcatc tgacggccaa tacatcgctg tcacatcgag tcttgatgga
      421 acgattcttc tctgggacct ggcgacgcgg cgagatcgca tggttttaca tcaaccctcc
      481 gccgtcgtga gcgtcgcctt ttcacccgac ggccggtggc tggcgacggc ggggagggtt
      541 aatcggtcga tcctgatatg ggacctccaa tgcggatctc cgcgatcatt gcaagaaggt
      601 gagcctgccg gcgttaccct agcgatcgcc ttctctcccg acggggctct gctggcctcg
      661 gccggcttgc ccgagcatca cgttcgcctc tgggaccttg agacacgtcg ggtgtgtcgg
      721 gaattcgcgg gacatgaacg ctcggtaaat tcggtcgcgt tttcaccaga cggctcactc
      781 ttggccaccg caggaaatga cggaatgctc gggctctgga cagtgacgac gggcgagcgc
      841 ctgctcagcc ttgacggcca ggccgggtat ccccgaaccg tcgcattctc gccggatgga
      901 cgaaccatcg ctctagcaac cgggaatgac gacgacatcc gactgtggta cctcgccgaa
      961 ctcaccagag cccggcctgg gtcgaactcg ttaccgatcg aacttggccc aataccaagg
     1021 caatgcgacc ggcggaactt gaaacgcgat ccgaccccat agacgtgacg cggcaatgcc
     1081 agccgattta agcgcgaggc cctgcaaccc gcgtaatgat cccacgattt ggcaagttcg
     1141 gagttcgttt attaggttca ccgagaaacc aagccttgca agttggggga tgctagacgc
     1201 ttgcgtctgg tgagaggaca cgcccgagcg cggtaccggc gacactttct caggcatggg
     1261 gtcgaagcgc aaggcagtgc gcgccgcctg ccgctgaacg aggacggatg agcgaaagcg
     1321 ccctaactca ataatcgcaa accggttcgg atttcaaaac gatgcccgcg ctcgccaatg
     1381 cccaaaccgc gagtgccaac gactcagctt caaccgtttc acgtcccaac accattatgt
     1441 gacggcgcct ttcgttcttt cgcatctcag ccgatccctc aaagggttcc ccagctcatg
     1501 cggccggggt ggaacgtgtg cagcgcgacc gggtcttcca cgccgactcg agtggagtcg
     1561 agagccggga agccgctcac ggcccggcga agaggtcggc gggctggtcg ggcatcccca
     1621 gtctggcctt ccacgcggtg gcctgctcgg tcttgcccca gtcctcgtac aatcgcaccg
     1681 cccgctcggc ggcctcgcgc aggcggaacg tctccggcac tgcgattttc gcctcacgcc
     1741 gcttcacccc ttcgtagccc ggcacaatca gcggctcggc ctcggcgcag cggccctggc
     1801 caagcagcgc cccgccgagc tggctcatcg catcgtaccg ggaccagtca tcgggcaccg
     1861 ctttctcccg gatcgccagg ccctcgcgca agatgggctc ggcctcggac caccgcgact
     1921 gcatcagcag gtttcggccg agcgcggcga ggtcgcc
//
LOCUS       DATAQL010001185         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5464017, whole genome shotgun sequence.
ACCESSION   DATAQL010001185 DATAQL010000000
VERSION     DATAQL010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5464017"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..2240
                     /locus_tag="VHS97_20420"
     CDS             504..2240
                     /locus_tag="VHS97_20420"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX3450632.1"
                     /translation="MSKSRSRNASTEPPSDVRDRPAAPGVFPDLLPHLWRFRTQFREV
                     CDADKVLRAALRLGLDFFRAQEGCVATLRPGGGEARILFPVPADGWWDRLMLAGFLRG
                     DDKVRVPPELMLARIRRHGRRWGALAVRSPNGYHWDQRQAFSTIGALANELIDQIDQE
                     RVREVRARVDRKVLEQSLPKHLFYEVLHGIRSLTAYDHSAALLTYDHEVDGLEVVAEQ
                     ITWQKAKGRNVGRKLPMTAEVRDQLCRPLVCGFDRNGASWKDWTGTDATPLAHLLDFD
                     VHAAVIGLAPREGAFLCAPLVTRAGLLGVLKVAAIEPGSFSQYEVELVSQFLPQAAVA
                     LQNARRAESLQQRVLIAERKHAMADLARGVSHDVNNALGAVLPLVQQLRAEIDQGTFD
                     PAMVAADLAQFERSIQVCRRIFGGMLNFARGSAKNISEVSLRHAADSVLAILRESLDR
                     RQVNLIVEIPDDLPLLNGVQADVEQLLLNLVSNARDATEAGDRLTIKAKGNGTAIELV
                     VEDTGCGIPKEHLNKIQEPFFTTKADGHGLGLAICRSIVAQMRGQFQIESNPGVGTRI
                     RATLPAQAEGAS"
     gene            2237..3385
                     /locus_tag="VHS97_20425"
     CDS             2237..3385
                     /locus_tag="VHS97_20425"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX3450633.1"
                     /translation="MIVEPHGARILVVDDDPGILHALSRILGRRHKVTCVASGPAALE
                     EAQHLRPDLAIVDIRMPEMTGFEVTRGLKAALRDVDVILMTGNAEEPDDNLIQAIDEG
                     AFYFIQKPFDRRVLLALVTRCLELRRLREEREKFLGRVERELEEARRFQQSLLPPPHF
                     DRAGLSIDARYLACTELAGDIYDYVTAPDGTVALLIADVVGHGTSAAMMTAVVKAAFR
                     ASHGDGFEPRAVVERVQEGIRDFDPARFVTLVCGRLDTKKHELTYVNAGHPEPIARTK
                     CTQPVLLSPTAPLLTSALSDLPCEQASVPFEPGDSLLFYTDGVTEAWGPTGMFGPERL
                     VAMIMRGDRRGPELLDGILKEVATFTGSSTHQDDVTLLSLELAGNVSA"
     gene            3565..>3774
                     /locus_tag="VHS97_20430"
     CDS             3565..>3774
                     /locus_tag="VHS97_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450634.1"
                     /translation="MWGEDPEPYLTGGVNLVPLAPLTNVSENDLPALVRRMDARISAQ
                     PRPEALKLWTATPLLNGFWTRACATG"
BASE COUNT          660 a         1213 c         1201 g          700 t
ORIGIN      
        1 atcctgctct tcgatcgcga aaatggtcgc gtgtttagcg ccacggcaaa gaccaggctc
       61 aagggggaca tgatgacttt ctcatacgaa ggtaacgaac tcccgggcgc actggacctg
      121 accatcgagt ccaacgtgga actgcaacct gcggcgaaat gatcggtgtg ggcccgcgcg
      181 tggcacgggc ttttagcccg cgatgcgccc cgacacgggc aagatgcccg tgccactctc
      241 tccccgccgg ggcgcgggcg atgacttatc tctcacgcca tgctctcgga agaggcgctg
      301 ccgttctttc ccgtcgcccc gcccggcggg gagagggtgc ccgaagggcg ggtgaggggt
      361 cgactgccgg cgtcaagccc ttcatctctg cccttccctg gcgtaccatg aaagccgcgt
      421 cgcgtgcact ggacgcggca gagcgcgttg aatgcgcacg tcgactcacc cgatcaattc
      481 gctggtccct tttggatttc ctcgtgagca aatcgcggtc acgcaatgcc tcgaccgagc
      541 cgccgagcga cgtccgggat cggccggcag cccccggagt cttccccgat ctcctccccc
      601 acctgtggcg atttcgcacc cagtttcgcg aggtctgcga cgccgacaag gtcctgcgag
      661 ccgccctccg gctggggctc gacttcttcc gtgctcaaga aggctgtgtt gcgaccctgc
      721 ggcccggcgg cggcgaggcc aggattctct tccccgtgcc ggcggacggc tggtgggatc
      781 gcttgatgct ggcgggcttt ctccgcggtg acgacaaggt gcgcgtcccc cccgaactca
      841 tgctggcccg tatcaggcgc catggacgaa ggtggggtgc gttggccgtg cgatccccga
      901 acggatatca ctgggaccaa cgccaggcgt tttcgacgat cggggccctg gccaacgagc
      961 tgatcgacca gatcgatcag gaacgggtcc gcgaggtccg cgcccgagtc gatcgcaagg
     1021 tcctcgagca gagccttccc aagcatctct tttacgaagt tctccacggc atccgttcgc
     1081 tgacagccta cgatcactcg gcggctcttt tgacctatga ccacgaggtc gatgggctcg
     1141 aagtggtcgc cgagcagatc acctggcaaa aggcaaaagg tcgaaacgtc ggccgcaagc
     1201 tgccgatgac ggccgaggtg cgcgaccagc tatgccgccc gttggtttgc gggttcgatc
     1261 gcaatggcgc gagctggaaa gattggacgg gcaccgacgc gacccccctg gcccatctgc
     1321 tcgatttcga cgtgcatgcc gctgtgatcg ggctcgcgcc tcgcgagggg gcctttctct
     1381 gcgcgcctct ggtcacccgc gccggcctgc tgggggtctt gaaggtcgcc gccattgaac
     1441 ctggttcgtt cagccagtac gaagtcgagc tggtttccca gttcctcccc caggcggctg
     1501 tggctctcca aaacgcgcgg cgggccgagt cgctgcaaca aagggtgctg atcgccgagc
     1561 gcaaacacgc gatggccgac ctggcgcgcg gcgtgtcgca cgacgtaaat aatgcgctcg
     1621 gggccgtgct ccccctcgtc cagcaattga gagcagagat cgaccagggc accttcgacc
     1681 cggccatggt cgcggcggac ctcgcccagt tcgagcggtc gatccaggtg tgccgccgca
     1741 tcttcggcgg aatgctcaat ttcgcccgtg gctcggctaa gaatatcagc gaggtgtcgt
     1801 tacgccatgc cgccgatagc gtgctggcaa tcctgcgcga gagcctcgat cgccgtcaag
     1861 tcaatctgat tgtcgagatc cctgacgacc tgcctctctt gaatggcgtc caggccgacg
     1921 tcgagcagct tctgttgaac ctggtcagca acgcccgcga cgcgaccgag gcgggcgacc
     1981 gattgacgat caaggccaag ggcaacggaa ccgcaatcga gctggtggtc gaagacacgg
     2041 gatgcggcat ccccaaggag cacctgaaca agatccagga gcccttcttc acaaccaagg
     2101 ccgacggcca cggactgggg ctggccatct gccggtcaat cgtggcgcag atgcgcggcc
     2161 agtttcagat cgagagtaac cctggagtgg gaactcgcat cagggcgact ttgcctgcgc
     2221 aagcggaggg tgcatcgtga ttgtggaacc gcatggtgct cgcatcctcg tcgtcgacga
     2281 tgatcccggc attctccacg cactatcgcg catcctgggc cgccggcata aggtgacctg
     2341 tgtcgcgtcg ggcccggcgg ccctcgaaga agcccagcac ctgcggccgg acctggcgat
     2401 cgtcgatatc cgcatgccgg agatgaccgg cttcgaggtc acccgcggcc tgaaggccgc
     2461 actccgggac gtcgatgtga ttctcatgac cggcaacgcc gaagaacccg acgacaacct
     2521 gatccaggcg atcgacgagg gagcctttta cttcattcaa aagccgttcg accgccgggt
     2581 gctcttggcg ctcgtgaccc gatgcctcga gctgcgccgg ctgcgggaag aacgggagaa
     2641 gtttctcggt cgggtcgagc gcgagctcga agaggcgcgc cggttccagc agagcttgct
     2701 ccctccgcct cacttcgacc gtgcgggcct ctcgatcgac gcgcgctacc tcgcctgcac
     2761 cgagctggcc ggtgatattt acgactatgt cacggcgcct gacggcaccg tcgccctctt
     2821 gatcgccgat gtggtgggcc acggcacctc ggcggcgatg atgaccgcgg tcgtcaaggc
     2881 ggcgtttcgg gcatctcacg gtgatggctt cgagccgcgc gccgtggttg aacgcgtaca
     2941 ggaaggcatt cgcgacttcg atccggctcg tttcgtcacc ctcgtttgtg ggcgactgga
     3001 cacaaagaag catgaactga cctatgtgaa cgcgggccac cccgagccga tcgcccgcac
     3061 caagtgcacc cagcccgttc tgctcagccc gaccgccccc cttctgacgt cggccttgag
     3121 cgatctaccg tgcgaacagg cgagcgtacc gttcgaaccg ggtgactccc tgctctttta
     3181 caccgacggc gtcaccgagg catggggacc gacgggtatg ttcgggccag agcggctcgt
     3241 cgccatgatc atgcgcggcg atcgtcgcgg accggagctt ttggatggca tcctcaagga
     3301 ggtcgcaacc ttcacgggct cttcgacgca tcaggatgac gtgacgctgc tgtcgctgga
     3361 attggcaggg aatgtctctg cgtgattcca ggaaatggca cagcttattc ggcacggtcc
     3421 tccgcggcga attcaccgag ctaaccgaac ggggacggat ttcgtggaat tcaaccgctt
     3481 cgacgtttcg acgaaggaac tggtctggga tgatccggcc gcctggttgg aacgttttcg
     3541 aagtacattt atcgcgcggt gcggatgtgg ggcgaagatc ctgagcctta cttgactggc
     3601 ggggtcaacc tggtgccctt ggctccgttg accaatgtca gcgagaatga tttgcccgcc
     3661 ctcgtgcggc gaatggatgc gcggatcagc gcccagccgc gaccggaggc gttgaaactc
     3721 tggacagcca cgcccttgct gaacggattc tggacccggg cgtgcgcgac tgga
//
LOCUS       DATAQL010001186         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3478111, whole genome shotgun sequence.
ACCESSION   DATAQL010001186 DATAQL010000000
VERSION     DATAQL010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3478111"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VHS97_20435"
     CDS             complement(<1..636)
                     /locus_tag="VHS97_20435"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX3450635.1"
                     /translation="MSGPDVDMSPERSTSGRQGPVRRRVLTINGGSSSLKFAVFGADG
                     ALAREVSGRVERIGQAGSRLILRDSSGVPREDTPIEVPDQAAAAHLVLDRLGSDPGLD
                     AISAVGHRVVHGGDRFVEPARISAEMVEGLRRIAPLDPEHLPGEITLIEAFGRAIRAV
                     PQVGCFDTAFHRNMPRSARIVPIPRRYWALGIRRYGFHGLSYAYLMEELARI"
     gene            complement(1167..1238)
                     /locus_tag="VHS97_20440"
     tRNA            complement(1167..1238)
                     /locus_tag="VHS97_20440"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1204..1206),aa:Gly,seq:ccc)
     gene            complement(1378..>1640)
                     /locus_tag="VHS97_20445"
     CDS             complement(1378..>1640)
                     /locus_tag="VHS97_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450636.1"
                     /translation="HSALVSRLADAASRVAAAAHIPPTVAIISGSGEFLARAVARRLA
                     GVSSILSLCELWGPHASITACARGLLGLACKRFPSRHLSAET"
BASE COUNT          285 a          528 c          558 g          269 t
ORIGIN      
        1 aattcgggcg agctcttcca tgaggtaggc gtaggagagg ccgtggaacc cgtagcggcg
       61 gataccgagc gcccagtatc gtcgcgggat cgggacgatc cgcgcggacc gcggcatgtt
      121 gcgatggaac gccgtgtcga agcagccgac ctgcgggacg gcgcgaatgg cccgtccgaa
      181 cgcctcgatc agggtgatct cgccgggcag gtgctccggg tccagcggag cgatccggcg
      241 cagcccctcg accatctcgg ccgagatccg cgcgggctcc acgaagcgat cgccgccgtg
      301 gacgacgcga tggccgacgg ccgaaatcgc gtcgagcccg gggtcgctcc cgaggcgatc
      361 gagcacaaga tgtgccgcgg cggcctggtc cgggacttcg atcggggtgt cctcgcgcgg
      421 gacccccgac gaatcgcgga gaatcagtcg cgacccggcc tgcccgatac gctcgacgcg
      481 accggagact tccctcgcga gcgcgccgtc ggccccgaac acggcgaact tgaggctgga
      541 ggagccgccg ttgatcgtca agacgcggcg ccgcacaggc ccctgacggc cgctcgtcga
      601 tcgctcggga ctcatgtcga cgtccgggcc gctcatgggt ctggacccct ccgcttcgcg
      661 aactcgcggc gcgtccgctg agtgatgaag tcgatccttc ggcgttccgc ctcggcgcgc
      721 ctggcacgcc cggacctggt cttgggcctc ccggacgatc gcgtccagtc ggctttcggg
      781 agtcatggtc gcaggcgtcg ccgttctcca gtcctccttc cccgtcgtac ctggccacct
      841 ggacgggcgg caggatcgtc gatcgggcgg cgggcctgac gtccgttggc ggctgaggac
      901 tcaccggagc aaggccaagt cggtctaccg gcccaagccg gaatgacatt aacagaatca
      961 gaataccaac gcatctgcca aacctggcag atgcattgtc gcgccggatt cgttcaggcg
     1021 gtcggacgta ggctggggta tggcacgtcg aaccgtgctt gcccaaaacg gcggttctag
     1081 cccccgttgg cttgtgccgt gcttgtcccg gttgcaccta tgtcgtgaag atggtgcaac
     1141 cagaataaag acttgttgtc agactaagcg ggcgagggga ttcgaacccc tgacgtccag
     1201 cttgggaagc tggcattcta ccactgaatt acgcccgcat tgtggccggc gattaccgga
     1261 cactcaagct gccaggagtt tatcagcggt cgggagcatc gacaaggcac ttagaagatt
     1321 tctcgcccga aggttgagtc tgtgaggcct ccgcaaggtg cccgtttcct tacatggtca
     1381 agtttccgcg ctcagatgtc tcgacggaaa acgtttacag gcgaggccga gtaggccccg
     1441 ggcgcacgca gtgattgagg cgtggggacc ccagagctca caaaggctca ggatcgacga
     1501 gaccccggcg agcctgcggg cgacggcgcg cgccaggaac tcgccggacc cggagatgat
     1561 cgccaccgtc gggggaatat gggccgcggc cgcgacgcgg gacgccgcat cggccagccg
     1621 gctcacgagt gccgaatgtg
//
LOCUS       DATAQL010001187         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_732206, whole genome shotgun sequence.
ACCESSION   DATAQL010001187 DATAQL010000000
VERSION     DATAQL010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732206"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VHS97_20450"
     CDS             <1..421
                     /locus_tag="VHS97_20450"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX3450637.1"
                     /translation="SVRRHRQVLAIACGVWVLAFALQVLPEGRVSFRGLPMIPLPQAC
                     FSRSWLGLKCPGCGLTRSIIQLAQGNWRASWQEHRLGALIAIVITLQIPYRIVALRRP
                     DRPLFSPGWQAALGYALIAVLVGNWLVDLASGRVTAI"
     gene            452..2005
                     /gene="zwf"
                     /locus_tag="VHS97_20455"
     CDS             452..2005
                     /gene="zwf"
                     /locus_tag="VHS97_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3450638.1"
                     /translation="MSESNALPEVNPLREALPRTRVSDPCAVVLFGATGDLAHRKLVP
                     ALFQLARGGNLPSECAIVGFARRDWTDADLRAEYEKTLSKSGGADFQQVWQQFANRIV
                     FSPGTFDDPASYQTLKETLDRVDETLGTHGNRVYYLAVAPEFFATIIKGLGESGLIYP
                     GHQDQPWSRVVIEKPFGHDLQSARALNRELSAVLDESQVYRIDHYLGKETVQNIHALR
                     FGNTIFEPVWNRRYVGSVQITVAEEVGMAGGRGAYYDTSGTIRDMVQNHMMQLLSLVA
                     MEPPVDLSADAVRNEKVKVLQALPRWRPDDVYRNVVRAQYTAGSIQGTEVPGYLQEKG
                     VAPDSKTDTYVAIRLELSNWRWAGVPFFLRTGKRLPKRATEIAIQFRRPPTELFEPEL
                     DGQSGANQLVLRIQPNEGASLTFEAKVPGSRRRLQEVRMDFRYGTAFAVPPPEAYERL
                     LLDVMLGDPTLYTRTDEVESAWRFITSILDAWQAPTAPPPVNYVAGSWGPAEADALIA
                     RDGARWRRL"
     gene            2173..>2764
                     /locus_tag="VHS97_20460"
     CDS             2173..>2764
                     /locus_tag="VHS97_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX3450639.1"
                     /translation="MIESTSTTDSFLDGQGIPVDLPHIETELAKLWGPAAEQIGGPEL
                     ENPNVTRIVLANLVVVCLDGDCESLGPVLETVIARFPCRAIVVRGSDDPERHINAEVS
                     ALCHLPAPGLPQVCSERILLRAGPQAIDLVPGAVRPLLEADLPMVLWWTSDPRKHEHL
                     FRDLAEECARLILDLPDPGADVGALRLGLDAGLSPSS"
BASE COUNT          489 a          854 c          866 g          555 t
ORIGIN      
        1 ctcggtaagg cgccatcgtc aggtgctagc gatcgcctgc ggggtatggg tcctcgcctt
       61 cgcgcttcaa gtacttccgg aaggccgtgt ttcgtttcgc ggtcttccca tgattcccct
      121 gccccaagcg tgcttttcgc gaagctggct cggcctcaag tgccccgggt gtggtctgac
      181 gcgttcgatt atccagttgg ctcaaggcaa ttggcgggct tcatggcaag aacatcggct
      241 gggcgcactc atagcgatcg taatcacgct ccagattccg tatcggatcg tggccctgcg
      301 ccggccggac cggccactct tctcgcccgg ttggcaggcg gcactgggtt atgctctaat
      361 cgctgttctt gtggggaatt ggctcgtgga cttggcgtct ggccgggtga ccgcgatcta
      421 gtggctcgtc agtatttgag agcgaatgcc gatgtccgaa tcgaatgcgc ttcccgaggt
      481 caaccctttg cgtgaggcct tgccgcgcac gcgcgtctcc gatccgtgtg cggtcgtgct
      541 tttcggagcg accggagacc tcgcgcatcg caagctggta ccggctttgt ttcagctcgc
      601 acgcggcggc aacctgccgt ccgaatgtgc catcgtcggc ttcgcccggc gcgactggac
      661 cgatgccgac ctccgcgccg agtacgaaaa gactctgagc aagagcggcg gggccgactt
      721 ccagcaggtt tggcagcagt tcgccaatcg gatcgtcttt tcgccgggaa ccttcgatga
      781 ccccgcctct taccagacgc tgaaagaaac tctcgatcgt gtcgatgaaa cactcggcac
      841 gcatggcaac cgggtctact acctggcggt cgctcccgag tttttcgcca caattatcaa
      901 gggcttgggc gaatcgggct tgatctatcc cgggcatcaa gaccagccct ggagccgggt
      961 cgttattgaa aaaccgtttg gccatgacct acagagcgct cgcgccttga accgcgagtt
     1021 gtcggctgta ctcgatgaga gccaggtcta tcgcatcgat cactacctcg gcaaagagac
     1081 cgtccaaaac atccatgcgc tgcggtttgg caacacgatt ttcgagcctg tgtggaaccg
     1141 gcgctacgtt ggctcggttc aaatcacagt cgccgaagag gtcggcatgg cgggcggccg
     1201 gggggcgtat tacgacacct ccggcacgat ccgcgacatg gttcaaaacc acatgatgca
     1261 gctactgagc ctggtggcga tggagcctcc tgtcgacctg tcggcagatg cagttcgcaa
     1321 tgagaaggtc aaggtgctcc aggccttgcc gcgttggcgg ccggatgacg tgtatcgaaa
     1381 tgtggtccgc gcccagtaca cggccgggtc gatccagggg accgaggtgc ccggatatct
     1441 ccaggagaaa ggggttgctc ccgactccaa gaccgacaca tatgtggcca tccggctcga
     1501 gctttccaac tggcgatggg cgggggtacc cttctttctg cggaccggta agcgcctgcc
     1561 caagcgggcg accgaaatcg ccatccagtt ccgccggccg ccgaccgagc tttttgagcc
     1621 agagctcgac ggtcagagcg gtgccaatca gcttgtcttg cgcattcagc caaacgaagg
     1681 agccagcctg accttcgagg ccaaggtccc agggtcgcgc aggcgccttc aagaagtacg
     1741 catggatttc cgctatggca cagcgtttgc ggtcccgccg cccgaagcct acgagcgcct
     1801 gcttttggac gtgatgctgg gagacccgac gctttacacg cgcactgacg aggtcgagag
     1861 tgcgtggcgg tttatcacat cgattctcga tgcctggcaa gcgccaacgg ccccgccgcc
     1921 ggtcaattat gtcgccggca gttggggacc cgcggaggcc gatgcactta tcgccaggga
     1981 tggggctcgg tggcggaggc tctgagcggc cctcgatcaa attggtgtgg gccggccggt
     2041 ctcgccggta aaataaagta gaatcactga tgatcgactt ccccgctgga acactccgct
     2101 tggaccgcga cggtcacccg tgaccttctt ccccagtcga gcacggaggc cgcaccgtca
     2161 aggagcagcg caatgatcga atcgacgtca acgaccgact cttttctcga cggccagggg
     2221 atcccggtcg atcttcccca tatcgagacc gagctggcca agctctgggg gccggcggcc
     2281 gagcagatcg gggggccgga gctcgagaac ccgaacgtca cccgaatcgt gctggccaat
     2341 cttgtcgtcg tctgcctgga cggcgactgt gagtcgttgg ggccggtact ggagacagtg
     2401 atcgcgcgat tcccttgccg ggccatcgtc gtgcgcgggt cagacgaccc cgagcgtcat
     2461 atcaatgccg aagtgtcggc gctgtgtcac ttgcctgcac cggggctgcc ccaggtctgc
     2521 tctgagcgaa tcttgctgcg agccggtccg caggcgatcg acctggtacc cggcgccgtg
     2581 cggccgttac tcgaggccga cttgcccatg gtactctggt ggaccagcga tccgcgaaag
     2641 cacgagcatc tctttcgcga tctggccgag gaatgcgctc gcctgattct cgatttgccg
     2701 gatcccggag ccgacgtcgg agcacttcga cttgggctcg acgcgggact gagcccgagt
     2761 agcc
//
LOCUS       DATAQL010001188         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1190175, whole genome shotgun sequence.
ACCESSION   DATAQL010001188 DATAQL010000000
VERSION     DATAQL010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190175"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3081)
                     /locus_tag="VHS97_20465"
     CDS             complement(<1..3081)
                     /locus_tag="VHS97_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450640.1"
                     /translation="MTSPVPVRGRHRWLRVALAFVMMGAGLTAALPWLLALPSVQRRL
                     AAEANKILAPSSVEFGAIRLSWFRPTEISKIVLHDAHGDQLLAAPRATFGWNLWQVLV
                     NRPKAGVLTIDKGDLDIERFADGTVNLYETLRPVISEHPPVRLLIRVENGRLRFRDPL
                     FTDPVVADSAQVDLNLGRNSEPIFWDIRLAQTRAKGEPSKLDIEGSYSRADVDSSGRH
                     DLTLSLKGAQWPWTLANPLIQARGDLTGNLDGKRRAGRVHLVGDATITNLVAIGDLLS
                     TDTAHLDTLRAQLDLEGGNDAWTIDRLTVSSPVLSVQGQGSIPPTPRQGAWVEASVDL
                     AAVAKQLPSTLHLRNDLRVEHGTARLRADILLAADGHTQNWNISGKIADLAARQGEKM
                     LTLPDPASLTAKLERTATAMKLARLDVQSSFLTATGEGNLDDGIVVAANLDLAAFRER
                     FRDWIDLGPVELAGQGKLDARYQRHGSSFQAGVDAIFQDLRLGGLPLIGKLERNRATF
                     SGKLGGEATAAGWPVSWRDLSLQAATGELAIEGEARNDLKTKSLTVSGRARAPLGQGS
                     PGQRVEAEVKAMASQGVWTADRLSLALLRDSKLGPGVGADDAIRWEGKGRYDPRRDEL
                     VIESFAGPPRRPTEHDTWIAGNQRLRATGLASLGDAQVELAANTDLASLGPWLARPGA
                     SWNGQLDALVHARREQDLWNLGLRLTASDPESIAADGSNIGLGGNLVVGMNGAYAART
                     DRLELAELSVTAPYLQADGSGVIRDLSSQQATVDLKGSLNLDWDRIRKLIAQKIEPNA
                     RISGRPRPWRLAGVIDGLPAIDRIGSLEGDIGVQIDSLDVFGMRLSAVPVVLRAANGR
                     LDIDPIDAELNGGILHLEPELIYDKAGSTWVYLGKSSRLDGAIVNDEVSHRVLSFAAP
                     VLDGATRVEGRVSLALADAYFPILAAPGAQVRIDGDVLFDDVRFMPGPLADQLLSVFQ
                     RERRPLAVLRDPVNVRIADRKVYQQGLTIPVAKIASIGLDGSVDFDQKLDLVARF"
BASE COUNT          712 a         1082 c         1031 g          639 t
ORIGIN      
        1 aaagcgggcg acaagatcaa gtttctggtc aaaatcgacc gaaccatcga gcccgatcga
       61 cgcgatcttg gcgaccggaa tggtcaatcc ttgctgatag accttgcggt cggcgatgcg
      121 gacgttgacg gggtcgcgca aaacggcaag gggccggcgt tcgcgttgaa aaacgctgag
      181 caactgatcg gccaacggac ccggcatgaa ccgcacatcg tcaaacagca catcgccgtc
      241 gatacgcacc tgtgcaccgg gagcggcaag aataggaaaa taggcatctg ccaaggccag
      301 agagactcgc ccctcaacgc gcgtcgcgcc gtcaagaacc ggtgcggcga acgacaatac
      361 ccggtgcgac acttcgtcat tgacaatcgc cccgtccagc ctcgacgact tgcccagata
      421 cacccaggtc gagccggcct tgtcgtaaat gagctcgggt tccagatgaa ggatgccccc
      481 gttgagctcg gcgtcgatcg gatcaatgtc gagccgaccg ttcgccgccc gcaggacgac
      541 gggcactgcg cttaatcgca tcccgaagac gtccagggaa tcgatctgaa ccccgatatc
      601 accttccagc gagccgatgc gatcgatggc aggcagtccg tcgatcacgc ccgccaggcg
      661 ccaaggccgg ggacgcccgg aaattcgtgc gttcggttcg atcttctgag caatcagctt
      721 gcgaatccta tcccaatcca aattcaacga ccctttgagg tcgacggtgg cttgttggct
      781 tgagaggtca cgaatcacgc cggaaccgtc ggcttgcaag tagggcgcgg tcacgctcaa
      841 ttcggccagc tcgagtcgat cggtgcgagc ggcgtaggcg ccattcatgc cgacgacgag
      901 gtttcccccc agaccaatgt tcgatccgtc cgcggcgatc gattcggggt cgctcgcggt
      961 gagccgaagt ccgagattcc agagatcctg ttcacgacgg gcgtgcacca aggcatcgag
     1021 ctgaccattc caagacgcac ccgggcgagc cagccacggg ccgagtgacg cgagatccgt
     1081 gttggcggcc agttcaacct gagcgtcgcc cagtgacgcg aggccggtgg cgcgcagtct
     1141 ttggttgccg gcgatccagg tgtcgtgttc agttggacga cgcggcgggc cggcgaagga
     1201 ctcaatgacc agctcgtccc gtcggggatc gtatcgccct tttccttccc agcggatggc
     1261 atcgtcggcc ccgacgcccg gccccaactt tgaatctcta agtagcgcca gggatagccg
     1321 gtcagccgtc caaacaccct gagacgccat tgccttcact tccgcttcga cgcgctggcc
     1381 cgggctacct tgaccaagcg gcgctcgggc gcggccgctc acggtcagac tcttggtctt
     1441 taggtcgttg cgtgcttccc cctcgatggc taactcgcca gtcgcagcct gaagcgatag
     1501 gtctcgccac gataccggcc aaccggcagc cgttgcctcg ccgcccaact tccctgagaa
     1561 agtcgcgcga tttcgctcga gcttgccgat cagcggcagc ccgccgagcc gcaaatcctg
     1621 gaatatcgca tcgacgcctg cctgaaagct cgatccatgc cgctgatagc gcgcatcgag
     1681 cttaccctga ccagccagct caaccggccc cagatcgatc caatcgcgga accgttcccg
     1741 aaaagccgct aggtcgagat tggccgctac aacaatgccg tcgtccaagt tcccctcacc
     1801 ggtcgcggtc aaaaagctcg actggacatc cagccgggcc agtttcatcg cggttgcggt
     1861 acgctcgagc ttggcggtca agctggcggg atcgggcaga gtcagcattt tttcgccttg
     1921 tcgcgcggcc aggtcggcaa ttttacccga aatattccag ttctgggtgt gtccgtcggc
     1981 agccagaaga atgtctgcgc gcagccgtgc tgtcccgtgc tcgaccctga gatcgttgcg
     2041 caggtgcaac gtcgagggca attgcttggc cactgctgcc aggtccacgg aggcttcgac
     2101 ccaagcacct tgtctgggcg tcggcggaat cgacccttga ccctgaacgg ataatacggg
     2161 gcttgatacc gtgaggcgat cgatcgtcca tgcgtcgttg cccccttcca gatccagttg
     2221 tgccctcaac gtatcgagat gagccgtatc cgtggaaagc aagtcgccga tagcaaccag
     2281 attggtaatg gtcgcatcgc cgacaagatg cacgcgcccc gcgcgccgct tcccatcgag
     2341 gttgcccgtc aagtctccac gagcctggat cagcggattt gccagcgtcc aaggccactg
     2401 agcgcctttc agcgatagag tcagatcgtg gcgcccggat gaatcaacgt ccgcgcggct
     2461 gtagctcccc tcaatgtcaa gcttgctggg ttcccccttc gcccgcgtct gagcgagccg
     2521 gatatcccag aagatgggct cggaattcct ccccaagttc aaatcaacct gggcagaatc
     2581 ggcgacgacg ggatcggtga acaagggatc gcgaaagcgt agccggccgt tctcgacgcg
     2641 gatcaagagc cggaccggag gatgctcgga gatgaccggc cggagagtct catacaggtt
     2701 gacagtgccg tcggcaaacc gctcgatgtc gaggtcgcct ttgtcgatcg tgaggacacc
     2761 ggccttggga cgattcacaa gcacctgcca gaggttccag ccgaatgtcg cccgtggagc
     2821 agccagcagt tggtctccat gcgcatcgtg gagcacgatt tttgaaatct cggtcggcct
     2881 gaaccacgag agtcggatcg cgccaaactc cactgagcta ggggcgagaa tcttgttggc
     2941 ctcggccgcc aatcgccgct gcactgacgg aagcgccagt agccaaggaa gggccgcagt
     3001 taagcccgca cccatcatga cgaaagcaag cgcaaccctg agccagcggt ggcgaccgcg
     3061 aactggtacc ggtgatgtca tttcgaatgc ctgacctttc gccctcgatg gcacgggccg
     3121 cccgccggtg agactccacg atccagcaat tcagtgcatt ctatctgaat ccggggacgt
     3181 tcaccaattc tacgacgtgt tcgagcgatg cggagacgag atcaagggag acgtcaggag
     3241 acttggaacg ttgcaacggc atgggacatc tgccattgta tgcgatcctc tacgactgat
     3301 atgcagtcgg aagtaccaag aaacagacta gtcgccacgc gtcgcggaag tgagccaaaa
     3361 cgtggatcaa ctaaatgaag gcgcgaattc tgtgggtgag ccaagaggca gcattccgcg
     3421 tgccaatcgc tgggcctggg gcctctgctg gctcatgttt gctt
//
LOCUS       DATAQL010001189         4072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_580049, whole genome shotgun sequence.
ACCESSION   DATAQL010001189 DATAQL010000000
VERSION     DATAQL010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580049"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="VHS97_20470"
     CDS             <1..1714
                     /locus_tag="VHS97_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3450641.1"
                     /translation="NVGPGSPTGIVFGYGSRFPVKYQEALYICDWSYGKLYALHMAPK
                     GSTYTGELEEFLAGTPLALTDVVVNPKDGAMYFAVGGRNTQSGLYRVTYTSADAASVA
                     GDSSPATALMARDRRHKLESLHGHKDPHAVETAWPYLGHPDRFIRSAARVAIAFQDPA
                     LWRERALAETSNPEATLNALLALTQVSVQDPAHRKQDDASPDFKLRDQILLALDKLTW
                     ETLSRDQQLDLLRVYEVVLNRYGRPDESRQKKLIDRFDPYYPSRDRELNAELSQLLIY
                     LEAPDAAAKTVALLEQAPTQEEQIHYAQALRSLKGGWTPATRKAYFSWFVKASQYKGG
                     NSLRGFMANMKRSAVANLSEADKAELKPILDAKPASVASTSALPDHPFVKEWTMDELV
                     PAVGGGLTDRDYDRGRALFASTKCFACHRCNDEGGGVGPDLTGIAGRFNTKDLLESIV
                     VPSKTISDQYESVTIATSDGRVITGRIVNLHGDNLAINPDMLDPNNMVNVPRSQIEEI
                     KRSPVSMMPLGLLNTLHKDEVLDLVAYLLSRGDRQSPMFRRGGGATGSVSPGGARGAA
                     GAAR"
     gene            2162..3187
                     /locus_tag="VHS97_20475"
     CDS             2162..3187
                     /locus_tag="VHS97_20475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3450642.1"
                     /translation="MAIGKDGAVLRQLRTLYDVGTTQELTDGQLLERFATDRDQAAEL
                     AFGVLVERHGPMVLRVCRGVLADPHDTQDAFQATFLVLVRKARALWVRDSLGPWLHQV
                     AYRTAACARSAIARRQRHERRAARPENVVGDEPAAELERLLHYEIDRLQERYRAPVVL
                     CDLEGKSHEQAARHLGWPVGTVKSRLSRGRERLRSRLLKSGVSPNAGLIATALSFDGS
                     MASIPAALVGSTDGAATRFLSSQTIVRGAAVLLAQGVIRSMFITQWLKTGSILLVVEA
                     AVAGVGLLAQNGKSGAQLRGDEQFEAAAAADASDFVVKAGKLKVAVIERGSLESSNQS
                     HLYSDLE"
     gene            3188..>4072
                     /locus_tag="VHS97_20480"
     CDS             3188..>4072
                     /locus_tag="VHS97_20480"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450643.1"
                     /translation="MTTIIRIVAEGTKVKKGDVVCELDSARLKDQLVNQRMTTASANA
                     ANENSKRMREIAELAVVEYADGILKQDLNTLKSEISAARSAIQKTESRLERTRRVREQ
                     LNGLQPRNGAAKSRDEIMVDLDLEDRLEEAEQRLLRDRMAFELATTKLELLQKYSQSK
                     TIKSLQVEVERNRSDELAKRAKWKLEKSKEAKLERQIAACTLVAPVDGLVVYGKAPGQ
                     FADQAQIEEGATVRLRWKVVTIHDLSRIQVLVKLPESHVDQIAPNMIARIRVDAFPDQ
                     LFTGIVRDVAPRADRSNFS"
BASE COUNT          825 a         1230 c         1263 g          754 t
ORIGIN      
        1 caacgttggg cctgggtctc ccaccggcat cgtcttcggt tacgggtcca ggtttcctgt
       61 gaagtatcaa gaagccctct acatctgcga ttggagctac ggcaagctgt atgccctcca
      121 tatggcccct aagggttcga catacacggg tgaattggag gagtttttgg cgggcacgcc
      181 tctggccttg actgatgtcg tcgtcaatcc caaggacggt gcgatgtatt tcgcggtggg
      241 aggacggaac actcagtccg ggctctatcg agtcacctat acgagcgccg atgcggcgtc
      301 ggtcgcggga gacagttcac ctgccactgc cctgatggcg cgcgatcggc gccataagct
      361 cgagtcgctg cacggccaca aagatccgca cgcggtcgag accgcgtggc cgtatctggg
      421 acaccccgac cggtttatcc gctcggccgc ccgggtggcg atcgcgtttc aagatcccgc
      481 gctttggcgc gaacgagctc tcgccgagac atcgaatccc gaggcgacgc ttaatgccct
      541 gctcgcgctg acccaggtca gtgttcagga tccggcacac cgcaagcaag acgacgcttc
      601 tcccgacttc aagctccgcg accagattct gttggctctc gacaagctca cctgggaaac
      661 gttgagccgt gatcagcaac tggacctctt gcgcgtgtac gaagttgttt tgaatcgtta
      721 cggccgtccc gacgagagtc ggcaaaagaa gctcattgat cgattcgacc cgtactatcc
      781 gtcgcgcgat cgcgagctca acgccgagct gagccagttg ctgatttacc tcgaggctcc
      841 ggacgcggcc gccaagacgg tcgcgcttct ggagcaggcg cctacgcagg aggaacagat
      901 ccattacgcc caggctttgc gatcgctcaa ggggggctgg acgccggcga cgaggaaggc
      961 gtatttctcg tggttcgtga aggcgagcca gtacaagggg ggcaacagtc tgcgcggctt
     1021 tatggcaaat atgaagcgca gcgccgtcgc gaatttgagt gaagccgaca aggcggagct
     1081 gaaaccaatc ctcgatgcca agcccgcgtc ggtggcatcc acttcggccc tgccagacca
     1141 tccattcgtc aaggagtgga caatggacga gctggtgccg gctgtcggtg gcggtctgac
     1201 cgatcgtgac tacgaccgcg gccgagccct cttcgcgtcc accaagtgct tcgcatgcca
     1261 tcgctgtaac gacgagggag gcggcgtcgg gcctgacctt acgggcatcg ccgggcggtt
     1321 taacaccaaa gacctgcttg aatcgattgt cgtcccctcg aagacgatca gcgatcaata
     1381 cgagtcggtg acgattgcaa ccagcgatgg ccgtgtgatc acgggccgga ttgtcaatct
     1441 gcacggcgat aatctggcga tcaatcccga tatgctcgat cccaacaaca tggtgaacgt
     1501 tccacgcagc cagatcgagg agatcaaacg gtcgcccgtc tcgatgatgc cgttgggtct
     1561 gctcaacact ttgcacaagg atgaggtcct cgacctggtt gcctacctgc tctcgcgcgg
     1621 ggaccgccag agcccgatgt tccggcgcgg cgggggagca actgggagcg tgtctcccgg
     1681 cggggcgaga ggagccgcag gcgcggcgag ataagagatt taggcccgat agcgttgaat
     1741 cagcaacgaa tttctgatgc atagcaattt atataatccc ctcccccctt gtgggggagg
     1801 gtagggtggg gggcgttcgt ctcgcgggcc gcaaccgagc ctatcttctc gcacctcggg
     1861 tccccccacc ccaactctcc cccacgaggg gggagggcga tggaaccgac ttcggcattg
     1921 taaacaatga ctggccacgt cctgaccgat cttctagcaa acgatgctcg ggggaacgcg
     1981 acccttcgcg ctggccgcat accctcgaag gagactcaca tgccagcctt gacatccgcc
     2041 gagccgcgtc caatgtccac gcaatcaacc tgaattttgc aaattgtgga acccacggcc
     2101 cgcgccgtta cattactcat atctgttgca aaaatggccg gacgacggag acttttttgt
     2161 cgtggcgatc ggcaaagatg gggctgtgct caggcagctc cgcacgctct atgacgtggg
     2221 gaccacccaa gagctgactg acggacagct tttggaacgg ttcgcgaccg atcgggatca
     2281 agccgccgag ctggcattcg gggtcctggt cgagcggcac gggccgatgg tcctgcgcgt
     2341 ctgccggggc gtgctggccg acccgcatga cacgcaggac gcctttcagg ccaccttcct
     2401 cgttctggtc cgtaaggccc gcgcactctg ggtacgggat tcgctgggcc cttggctgca
     2461 tcaggtcgct taccgcacgg ctgcgtgtgc ccggtcggcg attgcccgtc gacagcggca
     2521 cgaacgacgc gcggccaggc ccgagaacgt ggttggcgac gaacctgctg ccgagctcga
     2581 gcgactgctc cattacgaga tcgaccggct ccaggaacgc taccgcgcac cggtcgtgct
     2641 gtgcgacctt gagggtaaga gccacgagca ggcggcgcgg catctgggct ggccggtcgg
     2701 aacggtcaag agccggctgt cgcgcgggcg agagcggctg cgcagccggc tgctcaaaag
     2761 cggtgtcagc ccgaatgccg gactgatcgc gaccgccctg agttttgacg gctccatggc
     2821 gtcgatcccg gctgccctgg tcggttccac ggacggcgcc gccactcgat tcctttcgtc
     2881 ccaaaccatc gtccgaggtg cggctgtcct cctggcacag ggagtcattc gatccatgtt
     2941 cattacccaa tggctgaaaa ccggttcgat tctcctcgtt gttgaagccg cagttgccgg
     3001 cgtcggattg cttgcccaga acgggaagtc cggcgctcag cttcggggcg acgagcagtt
     3061 cgaagcggct gctgccgccg acgcctcgga cttcgtggtg aaggcgggca aactgaaggt
     3121 cgcagtgatt gaacgcggca gtcttgagag ctcgaaccag tcgcacctct acagcgacct
     3181 cgagtagatg accacgatca ttcggatcgt tgccgaagga acgaaagtaa agaagggaga
     3241 cgtcgtctgc gagctcgact cggcgcgtct caaggaccag ttggtcaacc agcgaatgac
     3301 aacagcgagc gccaacgccg ccaacgagaa ctccaagcgc atgcgtgaga tcgccgagct
     3361 cgccgtcgtc gagtatgccg acggtattct caagcaggac ctgaataccc tcaagagtga
     3421 gatctccgca gcccggtcgg ccatccagaa aaccgagagc cggctggaac ggactcgtcg
     3481 cgtccgcgag caattgaacg gactgcagcc tcggaatggc gccgcgaaga gccgcgatga
     3541 gatcatggtt gaccttgacc tggaggatcg cctcgaagag gccgaacaga gactcttgcg
     3601 cgataggatg gcgttcgagt tggcgacgac caagctcgaa ttgctgcaga agtacagcca
     3661 aagcaagacg atcaagtcgc tacaggtcga agtggaacga aatcgctcgg acgagttggc
     3721 caagcgggca aagtggaaac ttgagaaatc caaagaagca aagcttgagc ggcagatcgc
     3781 cgcctgcacg ctggtggccc cagtcgacgg cctggtcgtt tatggcaaag cgccgggtca
     3841 gttcgccgac caagcccaga tcgaggaagg cgcgacagtc cgccttcggt ggaaggttgt
     3901 taccatccac gacctaagcc ggattcaggt cctcgtcaag ctccccgaat cgcatgtcga
     3961 ccagattgca ccgaacatga tcgccaggat ccgagtcgat gcgtttcccg atcagctctt
     4021 tactggtatc gtgcgggacg tagcgccgcg agctgatcgt tccaacttca gc
//
LOCUS       DATAQL010001190         4771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1495627, whole genome shotgun sequence.
ACCESSION   DATAQL010001190 DATAQL010000000
VERSION     DATAQL010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495627"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1228)
                     /locus_tag="VHS97_20485"
     CDS             complement(176..1228)
                     /locus_tag="VHS97_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX3450644.1"
                     /translation="MPTEAMIQIQSNGHTFSLPTVAFQGLPSTLCKGCGHNSITSYLI
                     EACKSTGVNPYSTVKMSGIGCSSKTPAYFLQASHGFNALHGRMPSVATGAMVANSKLL
                     CIGVSGDGDTANIGMGQFKHVCRRNVPLVYIIENNGCYGLTKGQFSATADLNQHLRRP
                     TGETNDLPPFDLCAEAILGGATYVGRSFAGDKKQMVPMLKGALKHKGTSVLDIISPCV
                     TFNDFDGSTKSWDWAKEHEEPLQELGFVPRLPEIEVEQKAGTATRVQLHDGSWITLKA
                     LHHEEHDVTDKVSALRLLEESQRNGEFLTGLIYVDSKRRDFVSMQNTTPTPLALLADE
                     QLRPSKEVLEKIMETI"
     gene            complement(1402..3357)
                     /locus_tag="VHS97_20490"
     CDS             complement(1402..3357)
                     /locus_tag="VHS97_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX3450645.1"
                     /translation="MSSTLSVVEAAPARAGQATNAPIVNDLAIRVGTVNGSGSQSANL
                     AILRSLYAMGIPCSGKNVFPSNIEGLPTWFHIRASAKGYLAHILDPQILVCMNEQTAK
                     DDALALKPGSVCIYRDDFDVDLSQLRDDVRFFAVPFQKLVEKAYPPDKTDKGYRDKLR
                     KVINMVYVGVLAAVCRIEFEAVESGIRREFPGRKAKAGEINISAARTGFEWASANLPA
                     DLPFQVERMESARDKILIEGNKAAALGVLFGGVSVLTWYPITPSSSLAEYAEEFLKKH
                     RVAPDGTRTAAIIQAEDELAAVGMAIGAGWAGARALTATSGPGISLMSEFVGMAHFAE
                     IPVVIIDVQRMGPSTGLPTRTSQGDILKLHVLGHGDSRHIVLIPGTVEECYSLTIEAL
                     DLAQQFQTPVFMATDLDLGMNLWLTEPFPYPAKPITRGKVLNAEDLERLGHFERYKDV
                     DGDGVCYRTIPGTEHPLAAFFTRGTGHNEKSGYSERPEDWKKNLDRLVRKLETARAAL
                     PPPVIEGDPSAPVGLIAYGGTHFAIIEARDLLEGEGIPTEYCRIRALPAADDVTTFIK
                     RHLRVYVIEQNRDAQVTSILKSTLTGALADRLVPICHYNGTPIAAENIVRPILGWEKH
                     PSGPGWPTGDVERDNPPVAHDEQDSPE"
     gene            complement(3743..4300)
                     /locus_tag="VHS97_20495"
     CDS             complement(3743..4300)
                     /locus_tag="VHS97_20495"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3450646.1"
                     /translation="MATAEMLELMTAEQFGQRPDPGYPEELVRGRVVAMSVPDRRHGY
                     VCSKADRIIGNFVDTRGIGRVMCNDSGVITERGPDTVRAADVAYYSFSRLPKGTLPKG
                     YGPEVPELVVEVRSANDRWREILEKVSEYVNAGVLFVIVLDPESQIAHVFSADDAPKA
                     LIAEDELILPGVLDDFRVRVGRLFE"
     gene            4454..>4771
                     /locus_tag="VHS97_20500"
     CDS             4454..>4771
                     /locus_tag="VHS97_20500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3450647.1"
                     /translation="METDQVVRWRRRKDSRPAEILEAALDCFAERGFAATRLEDVAAR
                     AGVTKGTAYLYFKNKEDLFKAVVSGYLVPAIEQFEAAARAPGPISELLRSIAAMFFEK
                     VYKT"
BASE COUNT          916 a         1416 c         1467 g          972 t
ORIGIN      
        1 gggagaaatg cagcagtgtc gcgataccaa atcttcatca gcgcagggct gtggtcttta
       61 tcgggtcgcc cgagaagcct gcccggccaa agcacggccg ccgcaaagcc ggacgcccaa
      121 agaccgacgc caagcgattc acgtcacaat ctgatcgagc gaccttgtcg ctcgatcaga
      181 tcgtctccat aatcttctcg agcacctctt tcgacggccg caactgctca tcggccaaaa
      241 gcgccaacgg cgtcggcgtc gtgttttgca tgctcacgaa gtctcttcgt ttcgaatcca
      301 catagattaa tccggtgagg aactcgccgt tgcgctgact ttcctccaga agacggaggg
      361 ccgagacctt gtcggtgacg tcgtgttcct cgtggtgcaa ggccttcagc gtgatccacg
      421 agccgtcatg cagctgcacg cgcgtcgctg taccagcctt ttgctcgacc tcgatttccg
      481 gcaggcgggg aacgaaaccg agctcttgaa gcggctcttc gtgctctttc gcccagtccc
      541 agctcttggt cgagccatca aagtcgttaa aagtgacgca cgggctgatg atgtcgagca
      601 cggaggttcc cttgtgcttg agcgcgccct tgagcatcgg caccatctgc tttttgtcgc
      661 ccgcgaacga ccgccccaca taggttgcgc ctccgaggat cgcctcagcg cagagatcaa
      721 acgggggtaa atcgttggtt tcgccggtcg gccgcctcag gtgctggttc aggtcggccg
      781 tcgcggaaaa ctggcccttg gtcagaccgt aacagccgtt gttttcgata atgtagacaa
      841 gcggcacgtt gcggcggcac acgtgtttga actgacccat cccgatattg gccgtgtcgc
      901 catcgccact tacgccgatg cacaaaagct tggagttggc caccatggcg cctgttgcca
      961 ccgacggcat gcgtccgtgg agcgcattga agccatggct ggcctggagg aagtaggccg
     1021 gcgttttcga ggaacagccg attccgctca tcttcaccgt cgagtacgga ttgacgccgg
     1081 tcgacttgca agcctcgatc aggtagctcg tgatgctgtt gtggccgcac cctttgcaga
     1141 gggtcgaggg caacccctga aaggcgactg tcggcaacga gaacgtgtgg ccattactct
     1201 ggatctggat catcgcttcg gtgggcatcg tgtcgtcgcc tcgtgaaatc gatggtaggt
     1261 ggaagaaacc tcggttcgaa gccagttaag ccgggagcac gtcaaaggaa gagtgagata
     1321 ctcttcatct tactctttct ctcatgggcg agcggctagt gaacccgtcg actaaccatc
     1381 gacaggccgc aggccgctaa tttattcggg agagtcttgc tcgtcatggg caaccggagg
     1441 gttgtcgcgc tcgacgtcgc cggtgggcca gccggggccg gaggggtgct tttcccagcc
     1501 caggatcggc cgaacgatgt tttcggcggc gatcggagtt ccgttgtaat gacagatggg
     1561 gacaagccga tcggccagcg cgccggtcaa agtcgacttc aagatcgagg tcacctgggc
     1621 gtcgcggttt tgttcgatca cgtagactct caagtgcctc ttgatgaagg tcgtcacgtc
     1681 atcggcagcg ggcaaggcgc ggatgcggca gtattcggtg gggatccctt cgccctcgag
     1741 cagatcgcgg gcttcgatga tcgcaaaatg ggtaccgccg taggcaatca ggccaactgg
     1801 agcgctggga tctccctcga tgaccggcgg ggggagcgcg gcccgggccg tctcgagctt
     1861 gcgcaccagc cggtcgaggt ttttcttcca gtcctcgggg cgttcgctat agcccgattt
     1921 ttcgttgtgg ccggtacctc gcgtgaagaa cgcggcgagc gggtgctcgg taccgggtat
     1981 tgtccggtag caaacgccgt cgccgtcgac gtctttgtat ctctcgaaat ggccgagccg
     2041 ctcgaggtct tccgcgttca agaccttgcc gcgcgtgatc ggtttggcgg ggtaaggaaa
     2101 cggttcggtg agccagaggt tcatccccag gtcaaggtcg gtcgccatga acacgggggt
     2161 ctgaaactgc tgggccaggt cgagcgcctc gatcgtcagc gagtagcact cttcgaccgt
     2221 gccggggatc aagacgatat gccgcgagtc gccgtggccc agcacgtgca gcttgaggat
     2281 gtcgccctgg ctggtacggg tgggcaagcc ggtactgggt cccatgcgct ggacgtcgat
     2341 gatcacgacc gggatctcgg cgaagtgggc catgccgacg aattccgaca tgagcgagat
     2401 gcccgggccc gaagtcgccg tgagcgccct ggcaccggcc cagccggcac cgatggccat
     2461 gcccacggcg gccaactcgt cttcggcctg gatgatcgcg gctgtccgcg tgccgtccgg
     2521 agcgacccgg tgtttcttga gaaactcctc ggcgtattcc gccaagctgc tcgatggcgt
     2581 gatcggatac cacgtcagaa cgctcactcc gccaaagagc acccccaagg cagcggcctt
     2641 gtttccctcg atcaagatct tgtcgcgggc cgactccatg cgctccacct gaaacggcag
     2701 atcggcggga aggttggcgc tggcccactc aaagcccgtc cgggcggcgc tgatgttgat
     2761 ctcgccggcc ttggccttac ggccgggaaa ctcgcggcgg attcccgatt cgaccgcctc
     2821 aaattcgatc cggcagaccg ctgccaagac ccccacataa accatgttga tgactttacg
     2881 gagcttgtcg cgataaccct tgtcggtctt atcgggaggg taggcttttt ccaccaactt
     2941 ttgaaaagga accgcgaaaa accggacgtc gtcgcgcagc tgcgagaggt ccacgtcaaa
     3001 atcgtcgcga taaatgcaga ccgatcccgg ttttaacgcc agggcgtcgt ctttggcggt
     3061 ttgctcgttc atgcagacga gtatttgcgg gtcgagaata tgagcgagat atcccttggc
     3121 gctggcgcgg atgtggaacc acgtcggcaa gccctcgatg ttggagggaa agacgttctt
     3181 gccgctacag gggatcccca tcgcgtacaa acttcgcaag atggcaagat tggcagactg
     3241 actccccgag ccattgacgg tcccgacccg gatcgccaga tcgttaacaa tgggagcgtt
     3301 tgtcgcctgc ccggcgcgcg ccggggctgc ttcgaccacg gacaaagtgg atgacatgat
     3361 gttcctcgaa ctgaaggcgg tagaccccct ccaccccacg cggcagaccc aacatgcgtc
     3421 cgcgaggaat cgacattttc acaggaattc gaagcgggag ctcgacctgc cctcccatcg
     3481 atcagtcgaa cacattcggg gatcgtacgc aagggggaca tcggtcacct ggtaaaggtg
     3541 atgtgtgatg ccgggcacac tctgctgggt tcaaggcccg cggagcggcg tgggatgatt
     3601 gatcggggag ggcgaacgtc ctggcgagcc gagccaacgc cagctcggct ggagcctcgc
     3661 cctccccttg cggtttcggg atcatgccgc gcgggccgct ttgaaacgtc ccgggagggc
     3721 gaggtccggg cgaagcggtt cctcattcga agagccgccc cacgcggacg cggaaatcgt
     3781 cgagaacccc tggaagaatc aattcgtctt cggcgatgag tgccttgggc gcatcgtccg
     3841 cactgaagac gtgggcgatt tgggattcgg gatcaaggac gatcacgaac aagacgccag
     3901 cattcacgta ctcagagact ttctcgagga tctcacgcca acgatcgttc gccgagcgaa
     3961 cctcgacaac gagttcgggc acttcgggcc catatccctt cggcagcgtg cccttcggga
     4021 gtcgcgagaa gctgtaatag gcaacatcgg ctgctcgcac agtatccgga ccgcgctcgg
     4081 tgatcacacc cgagtcgttg cacatcacac ggccaatacc gcgcgtgtca acgaagtttc
     4141 cgatgattcg atcggctttg ctgcaaacat aaccgtgccg tcgatcgggc accgacattg
     4201 cgacgactct cccccgaacc aattcctcgg gataaccagg atctggccgc tggccaaatt
     4261 gttcggccgt catcaattcg agcatctcgg cggtcgccat cacgcacctc cctgaaggtt
     4321 caaccgttat tatcgccgat cgcctgctct cactccatac cagcgtgggg gggctctcct
     4381 cctcttgtag cgtcgttcac atgccggtaa aataaatgac tggtcagtta tcaagtgtcg
     4441 tcaggagcgt ttgatggaaa cggatcaggt ggttcgctgg cgccgtcgca aggattcccg
     4501 gcctgcggag atcctggagg ctgcgctcga ctgctttgcg gagcgcggct ttgccgccac
     4561 gcggctcgag gacgtggccg ccagggctgg cgtcaccaag ggtactgcct acctctattt
     4621 caagaacaaa gaggatctat tcaaggctgt agtgagtggc tacttggttc ccgccatcga
     4681 acagtttgag gcggcggctc gggctcccgg gccgatatcg gagctcttgc ggtcgatcgc
     4741 tgcgatgttc tttgagaaag tgtataagac g
//
LOCUS       DATAQL010001191         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4090027, whole genome shotgun sequence.
ACCESSION   DATAQL010001191 DATAQL010000000
VERSION     DATAQL010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4090027"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VHS97_20505"
     CDS             <1..1155
                     /locus_tag="VHS97_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450648.1"
                     /translation="QNVRRAFRSFPLHELPLADRTINPLTLYETYLAGGRVALLPFIL
                     ARAVAKTLVLAIRGIYRVVHEILHPHVDGQHIVPSDTYWGALRKIHRMRKPVFMGSLW
                     LRARFDVEYLGLPLPTAPPGTTALSLMETDLDYIGATRHDRIIAEQVRSQHQRRLEWV
                     LRWLDRFGWSFDELPAYLAREIPFLANRGGEALRALVAACVLDHNDIATLALSIGGLK
                     RMMAHAADAGEDLSKIPPGLPDPVVNLRRLWRPVNRCRRPVADLFELPCFPVYSVAER
                     RRVLRYLRRHRRVVRGWIKVVLGQGGRDPWATVKSRMRDVLLRTDLWSDQILVLRAVQ
                     SLTMLDLQHNCELVWNLGGYTRPEAGDSEPDAEPPRSPLFHEDHAVSARV"
     gene            complement(1197..2216)
                     /locus_tag="VHS97_20510"
     CDS             complement(1197..2216)
                     /locus_tag="VHS97_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450649.1"
                     /translation="MNDGMQPSSRPGELYDGTEGQVYRQWRLGVPVAVLSQQKGLSAS
                     VIERMINKTRAHAILGRPLEYMYHPSFEEPDAASAILAPMPGLPRTASKPKAPDGLPP
                     YLASLYNEAELLSRDQEAHLFRKMNYLKFRASNLREALDPARARASELDEIARLQEEA
                     LAVKNQLIRANLRLVVSIAKKRVGPSNDFFELVSDGNMSLIHAVEKFDFARGFKFSTY
                     ASWAIIKNYARANAEDRRRRDRFVTGHDELFEAAADHRSDEYEYESDRRRTQAAVQRV
                     LSRLNDREREILSSRFGLGGATELTLERIGRKLGVSKERVRQIESRAQNKLRAFALTT
                     KDTFE"
BASE COUNT          424 a          824 c          811 g          530 t
ORIGIN      
        1 caaaatgtgc gccgggcatt tcgcagcttc ccgctgcacg agctgccact ggccgaccgg
       61 acgatcaacc cgcttacgct ttacgaaacg tatctggcgg ggggccgtgt cgcgctgttg
      121 ccctttatcc tggcgcgagc agtggcaaag acgctggtgc tggccatccg cggcatctac
      181 cgagtcgttc acgagatcct tcatccccac gtggacggcc agcacattgt tccgtccgac
      241 acttactggg gggccttgcg caagattcat cgtatgcgaa agcctgtgtt catgggttcg
      301 ctctggctgc gcgctcgttt tgacgttgaa tacctggggc tgccgctgcc gacggccccg
      361 ccgggcacca ccgccctgtc gctgatggag accgacctcg attacatcgg ggcaacccgc
      421 cacgaccgga tcatcgccga acaagtccgc agccagcatc agcgacggct cgaatgggtc
      481 ttgcgctggc tggatcgatt cggctggagc tttgacgagc tgccggcgta cctggcgcgc
      541 gagatcccgt ttttggccaa tcggggcggc gaggctctgc gggctttggt ggccgcatgc
      601 gtcctcgacc acaacgacat cgccaccctg gccctttcga tcggcggcct caaacggatg
      661 atggcgcacg ctgcggacgc gggcgaagat ttgagcaaga tcccgcccgg gctgcccgat
      721 ccggttgtga atctacggag gttatggcgc ccggtgaacc gttgcaggcg cccggtggcc
      781 gacttgtttg agctcccctg ctttccggtc tatagcgtgg ccgaacgccg ccgagtgctc
      841 cggtacctgc ggcgacatcg ccgcgtggtc cgaggttgga tcaaggtcgt gctgggccaa
      901 ggggggcgcg acccctgggc tactgtcaag tcccgaatgc gcgatgtgct gttgcgcacc
      961 gacctgtgga gcgaccagat cctcgtcctg cgggccgtcc agagcctgac gatgctcgac
     1021 ctccagcaca actgcgagct cgtctggaac ctggggggtt atacccggcc cgaggccggc
     1081 gattccgagc cggacgcaga gccccctcgt tcgccgctct ttcatgagga tcacgcggtt
     1141 tcggcgaggg tgtaagacga gctggaaccg gtcgtccgtg agccagatcc ccgatgttat
     1201 tcaaaagtat ccttggtcgt cagggcgaac gcacggagtt tattctgtgc gcgcgattcg
     1261 atctggcgga cccgctcctt gctgactccc agtttgcggc cgatccgttc cagggtcagc
     1321 tcagtggcgc cgccgagccc gaagcggctg gacaggatct cccgctcacg gtcgttgagc
     1381 cggctgagca cgcgttgtac cgccgcctgg gtgcgccgcc ggtcgctctc gtactcgtat
     1441 tcgtctgaac ggtggtcggc ggcggcctcg aaaagctcgt cgtggcccgt gacgaaccgg
     1501 tcccgccgcc ttctatcttc ggcattggcg cgggcgtagt tcttgatgat tgcccacgag
     1561 gcgtatgtgc tgaacttgaa cccgcgcgcg aaatcgaact tctcgacagc gtggatgagg
     1621 ctcatgttgc cgtcgctgac cagctcgaag aaatcgttcg acgggcccac gcgcttcttg
     1681 gcgatcgaga cgaccagccg caggttggcg cggataagct ggttttttac cgccagcgcc
     1741 tcttcctgta gccgcgcaat ctcgtcaagt tccgatgccc gggcacgcgc cgggtcgagc
     1801 gcttcgcgga gattgctggc gcggaatttt agatagttca tcttgcggaa caggtgggcc
     1861 tcctgatcgc ggctgagcag ctcggcctcg ttgtagaggc tggccaggta cgggggcagt
     1921 ccgtccggcg ccttgggctt ggaggccgtg cgcgggagcc ccggcattgg ggcgaggatc
     1981 gccgaggcgg catcgggctc ctcaaagctc gggtggtaca tgtactcaag cggccgcccc
     2041 aggatcgcgt gcgcccgggt cttgttgatc atccgctcga tgaccgacgc cgacaggccc
     2101 ttctgctgcg ataaaaccgc gacgggaacc cccaggcgcc actggcgata gacctgacct
     2161 tcggtcccgt cgtagagctc gcccggtctc gatgacggct gcatcccgtc gttcaagatc
     2221 ggctccgccg atgtctcata cgtgtcatga agcatctttc gagctgtcat tgcaattttc
     2281 ccttccgcct caagaggccc atgcgcgctc cggcctctcc tgccgataaa gatcgtcgaa
     2341 ccggtcgtgt cgggatgcgg ttcgacgggt atttcgtaat cgctcgactt tctcacgaaa
     2401 tcaacaccgc atgtgaaacc acggggcctg aatgaggcgg tgctgcggta agacctaacg
     2461 cccctctaag ttccgcgaat tatgccggca aaacccttca tcgttttttc aataggggca
     2521 catcggtacc gccctgagtc agtagagttg ccgtaccgac tcccacccca cagctgtccg
     2581 ttcgaacag
//
LOCUS       DATAQL010001192         4854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4700263, whole genome shotgun sequence.
ACCESSION   DATAQL010001192 DATAQL010000000
VERSION     DATAQL010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4700263"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..2198
                     /locus_tag="VHS97_20515"
     CDS             258..2198
                     /locus_tag="VHS97_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3450650.1"
                     /translation="MSDSYVWRPTQAVMERSNIGRFMRRNGIAGYHDLVARSTSDIEW
                     FWNAAIEDLGIEFYRPYERLLDAGGGMPWSRWFVGGQINLVHNCLDRHIRSSRRNLTA
                     VIWEGEDASVRTLTYAELDSQTCRLAAALRRLGLGRGDAVGLYLPMVPEAVIAFLACA
                     KIGAIVVPIFSGFGAQAVAARLSDAGAKAIITADVSFRRGKAIEMEQVAALALADAPT
                     VRHVIVARNGRGTLGTPRAGGHVDWDELVAAETGELRTEPLDPEAPLMIAYTSGTTGK
                     PKGAVHVHGGFLVKIAQEVAHQVDMSADDRLCWVTDLGWIMGPWELVGGLAAGGTIVL
                     IEGAPDYPAPDRLWSLVARHHVTILGVSPTLIRALMRHGTEPVVAHDLSGLRILASTG
                     EPWDPESWRWLFENAGGGRCPIINISGGTEVGACLLSALPITPLKPCSLAGPALGMAV
                     DVFDASGQPLRRGVGELVCTKPWPAMTRGIWGDPDRYLATYWSRWPDVWVHGDWASID
                     DDGDWFLHGRSDDTLNVAGKRLGPAEVESILAEHGAVAESAAVGVPDTLKGEVIHCFV
                     VLKPGREPADSLRSELVERVALALGKSFAPDEIRFVEELPKTRSGKILRRLIRKVALD
                     LDLGDLSSVENVAALEAIRRSV"
     gene            complement(2360..3742)
                     /locus_tag="VHS97_20520"
     CDS             complement(2360..3742)
                     /locus_tag="VHS97_20520"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450651.1"
                     /translation="MDGSRYCWALAATVLPMLAPMPVVGQLAGDNPPSDLGRNHSPTR
                     KRLDLPVKKMTRRTRSRPPEQKEVAAANDWNPSYFRGRDHFIEGRYDEALTELVQNVA
                     ECREIDFDVKKSQNSYFDHVWTSMTQMGQSPHDFIRASNLQWVAAALAAQGRYDQAET
                     RFTEMENYAAKCFPGRYNTFEGCSCQGLAFLLAARGQYDEGAERYRLALAHIEGNQAQ
                     LGLPPAPCVAMILVALADVELARGQPDSAQRYLKRAEQIQEAQQILGIGPAPLDKAAL
                     HTVLAQLRINEERYSEAFDLHVQALSMIKEVRGDHPLTAYCLEGLGEIDLARGRLGPS
                     ADHFQESLAVRKRTLGESHRELAYSYDGLARVALAQEKYEAAESYFNDATLILARELG
                     PKHPDSIEVGNHARRRHDSSHSSGTTTRVRARFLAMPTLLTLGWQVLYVGKDWRGVEK
                     SIRQRDAKHK"
     gene            complement(3884..>4854)
                     /locus_tag="VHS97_20525"
     CDS             complement(3884..>4854)
                     /locus_tag="VHS97_20525"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX3450652.1"
                     /translation="SEYRSQQSSRHSRTLLRTAQLRDLPWPEREVNDPNNPRAKHQFH
                     GTDTEIDVVTEVGNEAFRAVVEFAMGSNHHGQSFLAREENGQIRELRIAHYPGAPEWD
                     RTMEHPLEPPDVPGYLGRPISAEAFRKCINCHSTNFRAVLEPDGRPEARDHGIGCERC
                     HGPGGHHPLAIAASFAEPAIARPRLASASRIVALCGECHTAPAKTTPEDPSFVRYQAS
                     GLILSRCYVESRDRLSCVSCHNPHQDLETRATSYEATCRKCHTPSRSLRQVPTRQTVD
                     EHNADMARTVCLVNAQNGCLDCHMPRIKNAVPRTEFTDHQIRVRRH"
BASE COUNT          830 a         1442 c         1571 g         1011 t
ORIGIN      
        1 aggtcgagct ggtcgccggg aaaatctatg caatgaccga cctgccaccg catcacagat
       61 gaaggtgagt ggctgctccc tcttcaattt gtggccgagc ggaaacgtac gcggggtcac
      121 gatacaccgc cagcactcac acccgcctcg catggaaatg tcctgatact tatcgtccct
      181 ccggcctggt aaactgtcag cgtcgtccct gtcgttcgtg cgaatcaacg tctcaaggcc
      241 gtctgatcgg aggttccttg tctgacagtt atgtctggag gccgactcag gccgtcatgg
      301 aacgttccaa catcggccgc ttcatgcgcc ggaatgggat cgccggttac catgacctcg
      361 tcgcccgatc gacatcggac attgaatggt tctggaacgc ggcgatcgag gatctcggta
      421 tcgagtttta tcgcccgtac gaacggcttc tcgacgctgg cggcggcatg ccctggtcgc
      481 ggtggttcgt cggtggacag atcaacctcg tccataattg cctcgaccgt catatccgat
      541 cctcacggcg caacctgaca gcggtcatct gggagggtga agatgcctcg gttcgtaccc
      601 tcacctatgc cgagctcgat tctcaaacct gccgactcgc cgccgccctt cgtcgactcg
      661 ggctgggacg gggcgacgcg gtcggcttgt atctgccgat ggtcccggaa gcggtgattg
      721 cgtttttggc gtgcgccaag atcggggcga tcgtcgtacc aatcttttcc ggatttggcg
      781 cacaagcggt cgcggctcgc ttgagcgatg cgggcgcgaa ggcgatcatc accgcggacg
      841 tcagtttccg caggggcaag gcgatcgaaa tggagcaggt cgcagccttg gctctcgccg
      901 atgctccgac ggtccggcac gtgatcgtcg cccgtaatgg tcgcggaacc ctaggcacgc
      961 cgcgcgccgg gggccatgtc gattgggacg agctggtcgc ggctgagacc ggtgagcttc
     1021 gtaccgagcc tttggacccg gaagcccctc ttatgatcgc gtacacgtcg ggcacgactg
     1081 gtaagccgaa gggggccgtg catgtccatg gcggttttct cgtcaaaatc gcgcaagagg
     1141 tcgcgcatca ggtcgacatg tccgcggatg accggctctg ctgggtgacc gacctgggct
     1201 ggatcatggg cccctgggag ctggtggggg ggctggccgc aggcgggacc atcgttctaa
     1261 tcgaaggggc gcccgactat ccggcgccgg atcggctctg gtcgcttgtt gcccgacatc
     1321 atgtgacgat tttaggagtt tcgccgacct tgatccgtgc cttgatgcgg cacggcaccg
     1381 agccggtcgt tgcacacgat ctgagcggac tgcggattct ggcgtcgacg ggtgagccct
     1441 gggatcctga gtcgtggcgt tggttatttg agaacgccgg cgggggccgc tgtccgatca
     1501 tcaatatctc gggcggcacc gaagtgggcg catgcctctt gtcggcgctg ccgatcacac
     1561 cgctcaagcc gtgctcgttg gccggacccg cgctggggat ggccgtggac gtgtttgatg
     1621 catcgggcca gccgctgcga cggggcgtcg gcgagctggt ttgtaccaag ccgtggccgg
     1681 cgatgacccg aggcatctgg ggtgatcccg atcgctatct ggccacctac tggtcgcgct
     1741 ggcctgacgt gtgggtccac ggtgattggg cctccatcga cgacgacggc gactggttcc
     1801 tccacggccg cagcgacgac actttgaacg tggccggcaa gcggctgggt ccggcggaag
     1861 tggaatcgat tctcgccgag catggagccg tcgctgagtc ggccgcggtc ggtgttcccg
     1921 acactctcaa aggagaagtg atccactgct tcgttgtcct gaaacccgga cgcgagccgg
     1981 ccgactcgct ccgatccgag ttggtcgagc gggttgcgct tgcgctgggc aaatcgttcg
     2041 ctcccgacga aattcggttc gtggaagagc tgcccaagac tcgcagcggt aaaattctcc
     2101 gtcgtttgat ccgcaaggtc gcgctggatc ttgacctcgg ggatctttcg agtgtggaaa
     2161 acgtcgctgc gctggaagcg atacgccgat cggtctgatt aagcgacctt gcacagatcc
     2221 gggacatttg tcgccacggt ttccggcaga cgtcggttgt aggtcgggct ttccagcccg
     2281 acatcttgtg cttcgcgtca cactgtctta gtcttatcgg tcaggctttc cagcccgacg
     2341 ccacgctgga aaggctgacc tacttgtgct tggcgtcacg ctgtcggatc gatttttcga
     2401 ccccccgcca atctttgccg acatacagaa cctgccaacc gagtgtgagc aaggtcggca
     2461 tcgccagaaa ccgcgcgcga acgcgcgtgg tggtgccgga ggagtgcgac gagtcgtgcc
     2521 tgcgacgtgc gtgattgccg acctcgatcg agtcgggatg ctttggcccc agttctcgtg
     2581 ccagtatcaa cgtggcgtca ttgaagtacg actcggcagc ctcgtatttc tcctgggcga
     2641 gcgctacccg cgccaggccg tcgtagctgt aagcgagctc gcgatgactt tcgccgaggg
     2701 tgcgttttcg aacggcgaga gactcttgaa agtggtcggc actcgggccg agccggccgc
     2761 gcgccaggtc gatttcgccg agaccttcca agcaataggc ggtgagagga tggtccccac
     2821 gaacctcctt gatcatcgaa agagcctgca cgtggaggtc gaatgcctct gaataccgtt
     2881 cctcgttgat gcgaagctga gccaggaccg tatgcagcgc ggccttgtcg agcggcgccg
     2941 gcccgatgcc caggatctgc tgtgcctctt ggatctgctc ggcgcgcttc aggtaccgct
     3001 gggcggaatc gggctggccg cgcgccagct cgacgtcggc cagagccaca aggatcatgg
     3061 ccacgcaggg ggccggtggt aggcccaatt gcgcctgatt cccttcgata tgcgccaggg
     3121 cgaggcggta acgttccgcc ccttcatcat attggccgcg ggcggcaagc aggaaagcca
     3181 gcccctggca cgaacaaccc tcgaaggtgt tgtaccggcc ggggaagcat tttgcggcgt
     3241 aattctccat ctcggtgaaa cgcgtctcgg cctggtcata tcgaccctgc gcggcgagcg
     3301 ctgcggcgac ccattgcaga ttgctggcgc ggataaagtc gtggggactc tgccccattt
     3361 gagtcatgct ggtccaaaca tgatcgaaat agctgttctg actcttcttc acgtcgaagt
     3421 caatctcgcg gcattcggcc acattctgga cgagctcggt caaggcctcg tcatagcgac
     3481 cctcgataaa atggtccctg ccgcggaagt aactgggatt ccagtcgttc gccgccgcga
     3541 cttctttttg ctcgggcggc cgtgagcggg ttcgcctcgt catcttcttg accgggaggt
     3601 cgaggcgttt gcgagtcggg gagtgattgc ggcccaggtc ggagggcggg ttgtcgcccg
     3661 ccaactgtcc aacgacaggc atcggagcca gcatcggcag gacagtagcc gccaatgccc
     3721 agcaataacg tgaaccgtcc attgctttcc ccctctctga cgcgtccgtg ccatagtgtt
     3781 cggccgttgt ccggggcgca acgcttcaca gattttaact tttcctggcc tcgcgtgtca
     3841 tcatcttgac gatggggcgg cgcgggcttg accgtccgcg tgttcagtgg cgccgaaccc
     3901 ggatttggtg gtccgtgaat tcggtgcgtg gaacagcgtt tttgatcctg ggcatgtggc
     3961 aatcgagaca accattctgg gcgttgacca gacagaccgt tcgagccata tccgcgttgt
     4021 gctcatcgac cgtttgtctc gtcggaactt ggcgcaacga ccgagaggga gtgtgacatt
     4081 tccggcacgt cgcttcgtaa gaagtcgccc gagtctcgag gtcttgatgc gggttatgac
     4141 agctcacgca gctcaggcga tcgcggctct cgacataaca gcgggagaga atcagaccgg
     4201 aggcctgata gcgcacaaag ctgggatctt cgggggtcgt cttggcgggc gcggtgtgac
     4261 attcgccaca aagtgccacg attcgcgagg cggaggccag ccggggacga gcgattgccg
     4321 gctcggcaaa cgaggccgcg atcgccagcg gatgatggcc tcccgggcca tggcaccgct
     4381 cacagccgat accatgatcg cgggcctcgg gccgaccgtc aggctcgagc accgcgcgaa
     4441 agttcgtcga gtggcaattg atgcactttc ggaacgcttc ggctgagatt ggccggccca
     4501 ggtaaccggg tacatccggc ggctcgagag ggtgctccat cgtccggtcc cactccggcg
     4561 caccggggta atgtgcgata cgaagctcac gaatttgccc gttctcttcg cgggcgagga
     4621 acgattgccc atgatggttt gagcccatcg caaactcgac gaccgcgcgg aacgcttcgt
     4681 ttcctacctc ggtgacgacg tcgatctctg tgtcggtgcc gtgaaattgg tgcttggccc
     4741 gcggattgtt ggggtcgttg acttctcgct cgggccaggg caagtcgcga agttgggcgg
     4801 tacggagcaa tgtccgcgaa tgtcggctgc tttgttggga ccgatactcg gatc
//
LOCUS       DATAQL010001193         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3174253, whole genome shotgun sequence.
ACCESSION   DATAQL010001193 DATAQL010000000
VERSION     DATAQL010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3174253"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VHS97_20530"
     CDS             complement(<1..459)
                     /locus_tag="VHS97_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX3450653.1"
                     /translation="MLIVLARNWWALALRGLVAVLFGIAAFAMPGITLTALVLLYGAF
                     ALVDGVFAVAAALFGRRHGMPWWAMLVEGLFGIAIGILTFAWPGITALALLYLIAAWA
                     VITGVFEIAAAIRLRQEIQGEWLLALSGALSVLFGVVLVLYPGAGALAVVW"
     gene            complement(647..1675)
                     /locus_tag="VHS97_20535"
     CDS             complement(647..1675)
                     /locus_tag="VHS97_20535"
                     /inference="COORDINATES: protein motif:HMM:NF022391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HEX3450654.1"
                     /translation="MRVHYGCVKWLLVMAGAAALQVVDANVWGQQSASSTTGQTSAKR
                     AEPVVRTYGGQGSFRTEVSSRTMGSLSEEDRRQVSLLMAQVFEHIQDARDAIDADDTK
                     KALDEVNKGREAIKAIRAMLPRTIVHTRTNAPDGKVLYEDERDVQDTYIPLYEGLLHT
                     ETLTPIIAAKRDALNVAGVHVVDAETIVTQARADLNIIESELTKAAKALGENKTDVAA
                     KALAPALVRGVDLRFSKEDSPLVVARDALWLARRALEENNAAQAIVNLTSAQQRLRIY
                     REVASQDERQEVDQMLREVAELDAQLRQERNQSASRATRAHQGNIIAQWWDKVNGWFR
                     GRSDHQSK"
     gene            complement(1767..2420)
                     /locus_tag="VHS97_20540"
     CDS             complement(1767..2420)
                     /locus_tag="VHS97_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3450655.1"
                     /translation="MKTDTELQRDVLEELKFEPSANAAHIGVAVNDGIVTLSGHVPSY
                     WEKYAAERAAKRVYGIKAIANEIEVKLPGSKQQADEEIAAAAVNALKSNISLPRDKIK
                     VIVSKGWLKLEGEVEWEFQREAAARAVRYLSGVTGVANLITVKPRVAPTELKAKIEDA
                     FKRSAELDARRLSIEVDGAKVTLHGTVRSLAEKEEAARAAWSAPGVSNVENLISVVP"
     gene            complement(2827..>3293)
                     /locus_tag="VHS97_20545"
     CDS             complement(2827..>3293)
                     /locus_tag="VHS97_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450656.1"
                     /translation="PQFESRLHRYLRDRIVARWDIGVHTPTPQILERAREEEQQFLER
                     QVQDTWRALQDDRPQRGALGPDEVFAALWQRRVQTLLDEPGVVRSGFRCSACGRLQLS
                     GGPCVECGGQNVAVPEVHEEAVHEAIEQDAQVRYWQSPALRNFDSIAAFQRF"
BASE COUNT          585 a         1056 c         1042 g          610 t
ORIGIN      
        1 ccagacgacc gcaagtgctc cggcgccggg atagagcacc agaacgactc cgaaaaggac
       61 ggagagggcc ccgctcaggg ccagcagcca ttccccttgg atttcctgcc gaagccgaat
      121 ggccgcggca atctcgaaaa cgccggtaat gacggcccag gccgcgatca ggtacaggag
      181 cgcgagcgcc gtgatccccg gccaggcgaa cgtgaggatc ccaatcgcga taccgaagag
      241 cccctcaacc agcatggccc accagggcat gccgtgcctg cggccgaaga gggccgcggc
      301 gactgcgaaa acgccgtcga ccagcgcgaa ggcgccgtaa aggagcacaa gcgccgtcag
      361 ggtgatgccc ggcatggcga aggccgcgat cccaaataac acggcgacca agccccgcaa
      421 ggccagcgcc caccagttgc gcgccaagac aatcaacaag atccacctcc ttttgcctca
      481 acaagaattg cagtccggtt tgacagcctc cgcgacgccc tacgccggtc agaatggcga
      541 cgcgtcggcg cgacgtgggt gttttcgttg agattggggc aacaggccga cccgacccgc
      601 gataccacgc gggctcgcgt ggtcgcgatc ggccatgaat cggccgttac ttcgattggt
      661 gatcagaacg cccacggaac cagccattga ccttgtccca ccattgcgca atgatgttgc
      721 cctgatgggc tcgcgtggcg cgactggctg actgatttcg ctcctggcgg agctgcgcat
      781 ccagttccgc gacctcgcgg agcatctggt ccacctcctg gcgctcgtct tgggaagcca
      841 cctcgcggta gatccgcagc cgctgctgag cggatgtcag gttgacgatc gcctgggcgg
      901 cgttgttctc ttccagcgcc cgccgcgcca gccaaagcgc gtcgcgggcc acgacgagcg
      961 gggagtcttc cttgctgaaa cggaggtcga cgccccggac caaggccggc gcaagcgcct
     1021 tggcggcgac atcggttttg ttttcgccca aggccttagc cgccttcgtc aactcactct
     1081 cgatgatgtt caagtcggct cttgcctgtg tgacgatcgt ctccgcatcg acaacatgaa
     1141 ctcccgcgac attcaaggca tcgcgcttgg ccgcgatgat cggcgtcaaa gtctcggtgt
     1201 gtaacagccc ctcgtaaagg gggatgtatg tgtcttgaac gtcgcgctcg tcttcgtaga
     1261 ggaccttgcc gtcgggggca ttggtccggg tatgaactat cgtcctgggc agcatggcgc
     1321 ggatggcctt gatcgcctcg cggcccttat ttacctcgtc aagggccttc ttggtgtcgt
     1381 cggcgtcgat agcgtctcgt gcgtcttgga tgtgctcgaa gacctgggcc atcagcagcg
     1441 agacctgccg ccggtcttcc tcactgagcg agcccatcgt ccggctcgac acctcggtgc
     1501 ggaagctgcc ctgcccgccg taggtccgaa cgacgggctc ggcgcgcttg gccgaggttt
     1561 ggccggtggt ggaggaagca ctttgctgcc cccatacgtt agcatcgacg acctggaggg
     1621 ccgccgcgcc agccatcacc aacagccact tgacgcaacc ataatggaca cgcatgatca
     1681 tacctcctga agcttgggtg tggggtgcga atcctcttag gacgaatgga actagacacg
     1741 caggccgacc gccatggcgg catggctcac ggcacgacgg agatgaggtt ttcgacgttg
     1801 gagactcctg gagctgacca ggcggcacgc gcggcttctt ctttttcggc caacgaacgc
     1861 acggtaccgt ggagcgtcac cttggcgccg tcgacctcga tggagaggcg gcgggcatcg
     1921 agctcggcgc tgcgcttgaa ggcgtcttcg atcttggcct tgagctcagt cggggccacc
     1981 cgcggcttga ctgtgatcaa gttggccact cccgtgacgc cgctcaagta gcggacggcg
     2041 cgggcggcgg cctcgcgctg gaattcccat tccacctcgc cctcgagctt gagccaaccc
     2101 ttgctgacga tgaccttgat cttgtctcga ggcaaagaga tgttggactt gagggcattg
     2161 acggccgccg cggcgatttc ttcatcggcc tgttgtttgc ttccgggcag tttgacctcg
     2221 atctcgttgg cgatagcctt gatgccgtag acgcgcttgg cggcgcgctc ggcggcgtat
     2281 ttctcccagt aggacggcac gtgcccgctc aacgtgacga tgccgtcgtt gacggcgacg
     2341 ccgatgtggg ccgcgttcgc gctcggctca aacttgagct cttccaggac gtcgcgctgc
     2401 aattcggtgt cggtcttcat gggtggctgc tccggttcgt tctgaatcgt tccgcggata
     2461 gcggagccgg ttggctccgc ccaaccgacc gatgccggcc ggtagccagt tcttttgcaa
     2521 cggagatgcc cgatcgcctc ttgtcgccaa gaagcgactc gggaccggtg ccaaaagccg
     2581 agatttccaa ggatttggag gtccacaccg ggacgcccaa agccgtgaat ttgcacaccg
     2641 aggcctgaac tcggccgaga caaactgtca ccgtgatcgg gccgaagaga cgttttgtca
     2701 ttgggatgac cgcgacggcg cgacgaaagc cattcgagcc aggacaagat cgcggaccag
     2761 gccagcaccg gcgaactccg cgacggatcc gcccgcggct tttggtgttt ccttccaggc
     2821 aaacggtcag aaccgttgga acgccgcgat ggaatcgaag tttcggaggg ccgggctctg
     2881 ccagtagcgg acttgagcgt cctgctcgat ggcttcgtgg acggcctctt cgtggacctc
     2941 gggaaccgcg acgttctggc cgccgcactc gacgcagggc ccgccgctga gctgcagccg
     3001 gccgcaggcc gagcagcgaa agcccgatcg gaccacgccg ggttcgtcga gcagtgtctg
     3061 gacccgcctc tgccaaagcg cagcgaacac ttcatcgggc cccagggccc cccgttgtgg
     3121 ccgatcgtcc tgaagggccc tccaggtgtc ctggacctgg cgctcgagga actgttgttc
     3181 ctcctctcgg gcccgctcca agatctgcgg ggtaggcgtg tgaacgccaa tatcccaccg
     3241 cgccacgatc cggtcgcgca agtagcggtg caagcggctt tcgaattggg gca
//
LOCUS       DATAQL010001194         6657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4700491, whole genome shotgun sequence.
ACCESSION   DATAQL010001194 DATAQL010000000
VERSION     DATAQL010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4700491"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VHS97_20550"
     CDS             <1..963
                     /locus_tag="VHS97_20550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3450657.1"
                     /translation="QGGVQWGSLTANAARFGSPILRQLYLASKMTNPKYWLYRSIEAK
                     QYHPDRHPRVVAVSTMVKRHIEQLHHVPRQQINVIPNAIDPKRLAVSHPGAVRCVFRN
                     RFGLEPGDLTGLFVGHNFALKGLRPLLEALGVRRQRNRQARPIHLVVCGSGPPGPFRR
                     LASRLGLNDTIHFLGFYPDVEACYWSTDFFVQPTYYDPCSLVVLEALACGLPVITTAQ
                     NGASELLNDGEEGYVLTTPDAREELIAALDHMANDTTRDAMSARALRLGQVQTFDIHV
                     ARLIAVFEDVAASRSRRSPHSRPRVSKSPALPHHEHGRKTGANN"
     gene            1068..1823
                     /locus_tag="VHS97_20555"
     CDS             1068..1823
                     /locus_tag="VHS97_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX3450658.1"
                     /translation="MKAVLLAGGKGTRLRPYTHALPKPLMPLGEDDPMPIIEVVLRQL
                     GRFGFGEVTIITGYLTELIEAFCGDGRKFGTRISYRRETTPLGTAGGLTLLERPREPV
                     LVINGDILTTLDYAEMFAFHSRRNAPATIASYPREVKVDFGVLVFGDDPHILTGYQEK
                     PEYSFEVSMGVYILDPLAWDFLTPGRALPMPDLLESMRHRGLDIHCYRQECYWLDIGR
                     HDDYATANEIFEARRGAFLGTKAATKLRIGRDQ"
     gene            1829..3670
                     /gene="plsY"
                     /locus_tag="VHS97_20560"
     CDS             1829..3670
                     /gene="plsY"
                     /locus_tag="VHS97_20560"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX3450659.1"
                     /translation="MPLVNSLALSALATVAAYLVGSIPFGYLVTYWARGIDIRTVGSG
                     NLGATNVGRTLGFRYFVLVLLLDLLKGLLPTLGFPILAGRITGQAPPDLPVVVALAAI
                     LGHTFPIYLKFRGGKGVATSLGTVLALDPIACAVALVVFAGSLLVTRYVSLSSLVGGL
                     AFVAAHFVRDSSPFSREHIAMSLFSITVLVLLFVRHRGNLVRIWAGTERRVSLHRGKP
                     GAGGQAHPSGKVVWAVVAGLVVIALGAVAGVEVYRQAAQPIEVNAGPWTLRETDRAAT
                     GQQRVDHVAFAGGGSRLAATCPRYDRLLVYSVEADGRLALVRDVELQGRPVALGTLGD
                     RYVVLESPSGDQRHIEPGWWETFDLGGNRVGGRHLAGYYPDDLAVSPDCKHLFVLSSG
                     CAEGDAKKPPPALDIIAVDIKAEPDRSVGRITFEATDDPCRLSLSSKAQCAAVLLTKT
                     NQTLAIDLSVPEAPQLVERIKATDSDAPYLSFSPDSDWIMMPVASQSESVAIDAPARD
                     HNANAAGMSDLPRQVQYQVCTRHRDSVLELFQNEPRQSLGRLPLKGPLNLGRTRPTGL
                     AYSRDRGLLAVATRSGTIHMVEMLPRTLSAVSGQGPIAASRGDAHRH"
     gene            3827..4327
                     /locus_tag="VHS97_20565"
     CDS             3827..4327
                     /locus_tag="VHS97_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450660.1"
                     /translation="MFRKKISSFNQMPRFCPDCARAAREDNESNLCADCGYTLIPQGY
                     CPICESYWRLAVGVPCPKHEILLDAGPPEQFEPIETDQAVSWVTVRVFPDSLSAAIPS
                     SRLEAEGIRTFLEGERMGSLEMYRVATGGVKLQVPAHQTAEARIILSQSWSLPGDEKA
                     DFEDLL"
     gene            4324..5820
                     /locus_tag="VHS97_20570"
     CDS             4324..5820
                     /locus_tag="VHS97_20570"
                     /inference="COORDINATES: protein motif:HMM:NF006113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HEX3450661.1"
                     /translation="MIKFVKRALLAIAVILSGLATISVIRTMAASSVQVVVEPVAKIS
                     IDDSAIERFAGAIRLHTVSRAEVPNSDPAALEAFHRHLQTCFPRVHATISPEVVGGHS
                     LLFTWKGKDQAAKPVLLMAHLDVVPVEPGTESSWVHEPFSGDVADGFVWGRGTLDDKA
                     SAMAILEAVETLLTKGFQPGPTILLAFGHDEEIGGHDGAAQIAALLKTRGTRLRYVLD
                     EGSAVTDGIVPGLTRPAALIGIAEKGFVSLELRAVAPAGHSSMPPPQTAVGIVAAAVR
                     ALENHPMPAALDGPAALLFDRLYPEMPFLTRLPLANRWLFGPLIVGQLERAPTTNAIL
                     RTTTAATLFEGGVKDNVLPAHARAVINFRIKTGETVNDVLSHATQVVNDRRVEIRVLE
                     PNWAKPASPQSSTSSRAFLTIERTIRQVMPEAIVAPSLVVAATDTGHYQELSEDVYRF
                     IPVVFGPDDPARLHGTNERVAIDVYRNCVRFYVQLLMNESHIPGANVP"
     gene            5817..5924
                     /locus_tag="VHS97_20575"
     CDS             5817..5924
                     /locus_tag="VHS97_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3450662.1"
                     /translation="MNRVISGIHHITAIAGDPQRNIDFYVGALGCASSS"
     gene            5921..>6657
                     /locus_tag="VHS97_20580"
     CDS             5921..>6657
                     /locus_tag="VHS97_20580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3450663.1"
                     /translation="MTVNFDDPFTHHLYYGDGHGSPGTILTFFPWPAAGRGSAGTGQV
                     TATAFSVPEGTLDFWVERLKNLKIDVDGPFTRFGEPLISISDPDGMKVELVATARGDA
                     DKSWNGGPVPVDSAIRGLHHATLSEEGYERTATLLTVTLGFELLAQEGNTFRYALGDK
                     APGVIVDVECAPDRPPGRVAVGSVHHIAWRTPDDPQQAVWRREIASLGYNVSPVMDRN
                     YFHSIYFREPGGILFEIATDPPGFTVD"
BASE COUNT         1193 a         2107 c         2022 g         1335 t
ORIGIN      
        1 caaggtggcg ttcagtgggg aagcctgacg gccaacgccg cccgctttgg ctcgccgatc
       61 ttgcgacagc tctacctggc ttccaagatg acgaacccga agtactggct gtaccggtcg
      121 attgaggcca agcagtacca cccggatcgt catccgcggg tcgtcgccgt gagcaccatg
      181 gtcaaacggc acatagaaca gcttcatcat gtgccacgcc agcaaatcaa cgtgataccc
      241 aatgcgatcg atcccaagcg actggctgtc tcacatccgg gggcagtgcg gtgcgtcttc
      301 cgtaaccgct ttggactgga gccgggtgat ctgaccgggc tttttgtcgg ccacaatttc
      361 gcactgaagg gcctacggcc gcttttggag gcgctcgggg tcaggcgtca gcggaatcgc
      421 caggccaggc ccattcacct ggtggtctgc ggcagcggcc caccggggcc gtttcgccgg
      481 ctggcgagcc ggctgggatt gaacgatacc atccactttc tgggtttcta tcccgacgtc
      541 gaggcgtgct actggtccac cgatttcttc gtgcagccca cttattacga tccctgttcg
      601 ctggtcgtct tggaagcact cgcgtgcgga ctgccagtga tcacgacggc ccagaatggg
      661 gccagcgagc tcctgaatga cggcgaagaa gggtatgttc tcaccacccc tgacgcgcgc
      721 gaggaactga tcgccgcgct cgatcacatg gccaacgata ctacacgcga cgcgatgtcg
      781 gcacgcgcat tgaggttggg ccaggttcag actttcgaca tccacgtcgc acgtttgatc
      841 gcggttttcg aggacgttgc ggcctcccgg tcgcgccgtt ctcctcattc acgtcctcga
      901 gtatcaaagt cgccggccct gccgcatcat gaacacgggc gaaaaacagg ggcgaacaac
      961 tgagagactt ttcgcgagtc cgggacacac actcgggccg ccgcgacgcg atagtaggtc
     1021 agcccttcaa ggctgacgtc atgctcgaaa gccaggaggg cactttcatg aaggccgtac
     1081 tgttggcggg aggtaagggg acacggctgc ggccatacac ccacgcgttg cccaagccgc
     1141 tcatgccgct gggcgaggac gacccgatgc cgatcataga agtggtcctc cgccagcttg
     1201 gccggtttgg ttttggcgaa gtgacgatca tcaccggcta tctgaccgag ctcatcgagg
     1261 cgttttgcgg cgatggacgg aagttcggca cacggatctc ataccgccgc gaaaccactc
     1321 ccctgggaac cgccggaggt ctgacgctct tggaacgccc tcgcgagccg gtgctcgtca
     1381 tcaatgggga cattttgacg accctcgact acgcggagat gttcgccttt catagcaggc
     1441 gcaacgcgcc cgcgacgatc gcctcgtacc cgcgcgaggt caaggtcgat ttcggcgtgt
     1501 tggtctttgg cgacgatccg cacatcttga caggatatca ggaaaagcca gagtattcat
     1561 tcgaggtgag catgggggtg tacatccttg acccacttgc ctgggacttt ttgacgccgg
     1621 gcagggcctt gcccatgccc gaccttttgg agtcgatgcg tcaccgcggc ctcgatatcc
     1681 attgctatcg ccaggaatgt tactggctcg atatcggccg acatgacgac tacgccactg
     1741 ccaacgagat cttcgaggcc cggcggggcg catttctggg caccaaagcg gccacgaaac
     1801 tccggatcgg gcgcgaccag tgaacgatat gccgctcgta aattcactgg cactctcggc
     1861 cctggctacc gtcgccgcct atctggtcgg ttcgattccg tttggctacc tggtcactta
     1921 ctgggccagg ggcatcgaca ttcgtaccgt gggatcgggc aacctgggtg cgacgaacgt
     1981 cgggcggacg ctcgggtttc gctatttcgt actggtcttg ctgctcgacc tgctcaaggg
     2041 tcttttaccc acgctgggct ttccgatctt agcgggccgg atcaccggcc aagcgcctcc
     2101 ggacttgccg gttgtcgtgg cgctcgcagc gatcctcggt catacgtttc cgatctatct
     2161 caagtttcga ggcggcaagg gagtcgcgac cagtctgggt accgtcctgg cgctcgatcc
     2221 cattgcgtgt gccgtcgcgc tcgtggtttt tgcggggtct ctgctggtga cacgctatgt
     2281 ctcactatca tcgctcgtgg gcgggttagc attcgtggcg gcgcatttcg tgcgggattc
     2341 ctcacccttc agtcgtgagc acattgccat gagccttttc tcgatcaccg ttctggtgct
     2401 cctgtttgtc cgccatcgtg gcaacctcgt ccgaatatgg gccgggaccg agcgtcgggt
     2461 cagcctgcat cgtggcaaac ccggcgccgg cgggcaggcc catcccagtg gcaaggtcgt
     2521 ctgggcggtc gtggccgggt tggtcgtgat cgccttgggc gcggtcgcag gagtcgaggt
     2581 ctaccgtcag gcagctcagc ctatcgaggt aaatgccggc ccgtggaccc tacgggagac
     2641 cgaccgagcc gcgacgggcc agcagcgggt cgaccacgtc gcttttgccg ggggcggcag
     2701 ccggctcgcg gccacctgcc cgcgctacga ccgccttctc gtgtacagcg tggaagccga
     2761 tggaaggctg gccttggtcc gcgacgtcga gctgcagggg cggccggtcg cccttggtac
     2821 attgggtgac cgatatgtcg tcctcgagag ccccagcggc gaccagaggc acatcgagcc
     2881 cggttggtgg gagacgttcg atctcggcgg caatcgcgtc ggagggcgac atctggccgg
     2941 ctactaccca gacgaccttg cggtctcacc cgattgcaag catctattcg tgctcagctc
     3001 aggatgtgcc gaaggggatg cgaaaaagcc cccgcccgcg ctcgatatca tcgccgtcga
     3061 catcaaggcc gagcccgatc gctcggtggg ccggatcacc tttgaagcaa ccgatgatcc
     3121 ctgccggctg tcgctttcgt ccaaggcgca atgcgccgcc gtcttgttga ccaagactaa
     3181 ccagaccctg gccatcgact tatcggtccc cgaggctcct cagttggtcg agcggatcaa
     3241 ggcgacggat tctgacgctc cctacctgtc cttttctccg gattccgact ggatcatgat
     3301 gccggtcgcg tcccaatccg aaagcgttgc gatcgacgca cctgcacgcg atcacaatgc
     3361 aaacgccgcc ggtatgagcg atttgcctcg ccaggttcag taccaggtct gcaccagaca
     3421 ccgcgactct gtcctcgagc tatttcaaaa cgagccacgc cagtcgctgg gacgcctgcc
     3481 cttgaaaggt ccgctcaatc tgggccgaac ccgacctacc ggtctggctt actcgcgcga
     3541 ccgcggattg ctggctgtcg ccacccgctc cggcacgatc cacatggtcg aaatgctccc
     3601 gcggacgttg tcagccgtct ctggacaggg accgattgcc gccagtcgtg gcgatgcgca
     3661 tcgtcactga cggtgctttg cgtgaaccaa ccggtccggg gcgtcgtgct ggccgactct
     3721 gcccgccgcg gttatgctga aaccggtgtg cgaggtcgat caccggcggc cagcaacagt
     3781 accagatcgc tcgccgagta ctcgccgacc ttggggacgc tcagcaatgt tccgcaagaa
     3841 aatatcatcg ttcaatcaaa tgccgcggtt ttgccctgac tgcgcccgag ccgcccgtga
     3901 agacaatgag agcaacctct gcgcggactg cggatacacg ctcattccac aggggtattg
     3961 cccgatctgc gagtcttact ggcgactggc ggttggcgtt ccctgcccga agcatgaaat
     4021 ccttctcgac gccggtcccc ctgaacagtt cgagccaatc gaaacggatc aggcggtctc
     4081 ttgggtgact gtgagagtct ttcccgatag tctctcggcc gcgatcccca gcagccggct
     4141 cgaggccgag ggcatacgga cctttctcga aggcgaacgg atgggaagcc tcgagatgta
     4201 ccgggtcgcc acgggaggcg taaagctcca agtgcctgct caccagactg cggaggcgcg
     4261 gatcattctc tcgcagagct ggtcgctacc cggcgacgaa aaagccgact tcgaagacct
     4321 cctatgatca agttcgtcaa gcgggcttta ctcgccatcg ccgtgatcct cagtggcctt
     4381 gcgacgatct ccgtgatccg gaccatggcg gccagttcgg tccaggtcgt ggttgagccg
     4441 gtcgcaaaga tctcgatcga cgactcagcc atcgaaagat tcgccggggc gatccgtctg
     4501 cataccgttt cgcgtgccga ggttccgaat tccgacccgg ccgcgctcga ggcctttcat
     4561 aggcacttgc aaacatgctt tcccagggtc cacgccacca tttcgcctga ggtcgtcggt
     4621 ggtcacagcc tgctttttac gtggaaaggg aaagaccagg cggccaagcc ggttttgctg
     4681 atggctcatc tggatgtcgt gccggtcgag ccgggaaccg agtcgtcttg ggtccacgag
     4741 ccattttcgg gcgatgtcgc cgatgggttc gtctggggcc gcggtacact cgatgacaag
     4801 gccagcgcga tggcgattct ggaagcggtc gagaccctgc tcaccaaagg ttttcaaccg
     4861 gggccaacga tcctgctcgc ctttggtcat gatgaagaga tcggtgggca cgatggcgcg
     4921 gcccagatcg cggcgctcct gaagacgcgc ggcacacggc tgaggtatgt tctcgacgaa
     4981 ggctcggctg tgaccgacgg aatcgtcccc ggcttaacgc gtcccgcagc cctgattggg
     5041 atcgccgaga aggggtttgt cagtctcgag ctaagggcgg tggcaccggc gggccattcg
     5101 tcgatgccgc caccgcaaac ggccgtgggc atcgtggcgg cggccgtgcg cgccctggaa
     5161 aaccacccca tgccggcggc gctggacgga ccggccgcgc tcttgtttga ccgcctctac
     5221 cccgagatgc cgttcctgac ccggcttccg ctggcgaacc gctggctctt cggccctctg
     5281 attgtcggtc agctcgaaag agcaccgacg accaacgcga tcctgcgcac cacaacggca
     5341 gcgaccttat tcgaaggtgg cgtcaaggat aacgtcttgc cggcccatgc acgtgcggtc
     5401 atcaactttc ggatcaagac cggcgaaacc gtcaacgacg tattgtcgca cgccacccag
     5461 gtcgtcaacg atcgccgggt cgagatccgg gtgctcgagc cgaactgggc caagcccgct
     5521 tccccgcagt cgagcacgag ctcacgcgcc ttcctgacga ttgaacggac gatccgccag
     5581 gtgatgcccg aagcaatcgt cgcaccttcg ctggtcgtgg cggccaccga caccgggcat
     5641 tatcaggaat tgtcggaaga tgtgtatcgg tttatccctg tggttttcgg ccccgacgat
     5701 cccgctcgcc tccacggcac caacgagcgc gttgccatcg acgtctaccg caattgcgtg
     5761 cggttttacg tgcagttatt gatgaacgaa tcgcacatcc ccggagcgaa cgttccatga
     5821 atcgtgtgat ttccggcatc caccacatca cggcgatcgc gggcgacccg caacggaaca
     5881 ttgactttta tgtcggcgca ctgggctgcg cctcgtcaag ttgacggtaa acttcgacga
     5941 cccgtttacg catcatctct actacggaga cgggcacggc agccccggca ccatcctgac
     6001 gttttttccg tggccggctg ccggccgagg ctcggccggg accggacagg ttactgcgac
     6061 cgccttttcg gtgcccgagg ggacgctcga tttctgggtc gaacgtctca aaaatctgaa
     6121 gattgacgtc gatggtccgt tcacgcggtt cggcgaaccg ttgatctcga tttcggatcc
     6181 ggatggcatg aaagtcgaac tcgtcgccac ggctcgcggc gacgcagata agtcatggaa
     6241 tggagggccc gtgcccgttg actcagccat ccgcggcctc caccacgcca cgctctccga
     6301 agaaggatac gaacgcacgg ccacgctctt gaccgtcacg ctcggcttcg agctcttggc
     6361 ccaggaaggc aacacctttc gctatgccct tggtgacaag gccccggggg ttattgtcga
     6421 cgtggaatgc gcgcccgatc ggccccccgg ccgggttgcg gtcgggagcg tgcaccacat
     6481 cgcctggcgc actcccgatg atccacagca agcagtctgg cgtcgggaaa tcgcgagtct
     6541 gggctacaat gtctcgcccg tcatggatcg gaattacttc cattcaatct actttcgcga
     6601 gccgggcggc atcctcttcg agattgcgac cgatccgccg ggtttcacgg tcgatga
//
LOCUS       DATAQL010001195         5628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3939085, whole genome shotgun sequence.
ACCESSION   DATAQL010001195 DATAQL010000000
VERSION     DATAQL010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3939085"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..2021
                     /locus_tag="VHS97_20585"
     CDS             90..2021
                     /locus_tag="VHS97_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450664.1"
                     /translation="MRSSQRIRRGAFCVGLAMLAGAGGALLSAGSPPSSNTQGKVDRS
                     DLIVHEWGTFLGMSGSDGASLDGMYHEEHALPAFVHSRSRDQLRLPSVFLKGETPVIY
                     FYTEQRQRVRVGVGFPHGIWTQWFPQALLVNPPLAQHAETPDRLRDGRICWQAEVIPA
                     SAVKPATGQRLENEQRPQIELPATSSDALWNYARDVDAAYVTTRDATKEKTPAEYEKF
                     LFYRGLGEARLPLRFDASSNGTLTLEHEIMLDEGVRHAFVIRVENGRAAYRYFPAVRP
                     GEQIHNAIPSMTQARPLAEFTRAISDELAAGLIKSGLYAKEARAMVNTWTASYFQTEG
                     VRVLFVLPQSWTDAFVPMTVSPAPQQIVRVMVGRLELLTADREKLAEGAVRNLTGGDA
                     GKRQDAFRFLHEQGRYVEPIVRRVMKTTTDDAVRTVCRRLLLTDFLTELRAAVHNAAD
                     GSRLTTDPLILRAHLARLLREVGSDTEARDLGTAILADLKRYPLAPNQALADNPAALE
                     IRAAAIEAMGDDRKAAAVYARRIELQIRSYKGGLNHGMIPGLRDWWVGRAYGQCVRRS
                     SQTASTTAALEKHLSGHPSTDRSDDLMPRVFLAYVLDGQGRPERADEEWRALAAEPKP
                     DQAAAAPALQPKEPGQTGT"
     gene            2158..2754
                     /locus_tag="VHS97_20590"
     CDS             2158..2754
                     /locus_tag="VHS97_20590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450665.1"
                     /translation="MSAAKDLTDPVLGARETVDLERFRAYLILLARLEIAPRLRDKVD
                     LSGVVQQTLLEAHQGLINYPGRPKTEAEITAWLRSILSHNLADVLRKLTTRKRDVRRE
                     WSLEQALDQSTSRLEQWLTVDQSSPSQRVMRQEEILRMAATLATLPEGQRRAIELHHL
                     QGWPLAEIAAELGTTKAAVAGLLHRGLKNLRMQLDPRQ"
     gene            2794..>5628
                     /locus_tag="VHS97_20595"
     CDS             2794..>5628
                     /locus_tag="VHS97_20595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3450666.1"
                     /translation="MASIPANSSDWSQEHAGEQEQTLDQVIADYLEAHEAGQPTNRDV
                     LLKQYPALAAELAHFFENQDRMARLTAQIRAGNQPGCSRGLESIFIPFPGPDHENSQQ
                     PDPLSSNEARVRYFGDYELIEILAQGGMGVVYKARQVSLNRVLALKMVGAGRFATPGD
                     LQRFRLEAEAAAHLDHPHIVPIYETGEHDGHHYFSMKLVDGGNLAAQVERYGHNPRAA
                     ARLVATVARAVHYAHERGILHRDLKPSNILLGGPAGAPLEERVPLVTDFGLAKRVEAR
                     HNPELTQSGSIVGTPGYMAPEQAEGHREAITTSVDIHALGAILYELLTGRPPFRAETM
                     LETLRMVREQEPVRPRTLKPRIDRDLETIVLKCLEKAPNQRYHSAEALAEDLERWLGD
                     LPIRARPTTLPQRAIKWARRRPAAAGLVLMATIASGIAIGGVIALRAEKEKHRKSEAE
                     LVESRARKIQMEEDRYFQQVIAADHAFEAKDPATAQRLLADCPPLLRNWEWYHLSRRL
                     HSELLTIVGHSGFVCPDFRPDTPDAECRTDALSGSLWDATDGPKLRRMHGPDGTAYGL
                     AIDRAGLRLATAGSDGQVKIWDMTRVRLLHVFRAHEGWTAGIAFSADGTRLALAGQDQ
                     TIRIWDVSSHPDAAGDRDGALQVLRGHTGGVFGVAFSPDGTKLASASKDGTARVWDLT
                     QQEARIALVFRGHDQEVCCLAFHPDGTRIASGGADRKVRIWDAATGRQILEFHAATSR
                     VNAIAFSSDGTKIATGSLDQPVDIWDAGNGHLISVLAGHTNPVFAVAFSSDGTRLISA
                     SQDATIKLWDLTSRPGVSRFELEREEASHSGDTVSTDPTMPTVRWVGGVAFRPSGDEF
                     AAAGSNQTIGIWDASTGRLKRKLHDGNATFFAMAYSRDGARLAAARTDRDVRIWDLNA
                     SRPPLVLSDAREGLASVAWSPDGNVIATGGG"
BASE COUNT         1122 a         1761 c         1740 g         1005 t
ORIGIN      
        1 tcatgcggcg gcatcgctca attaaacccc gtctgcaggt gtgagtcgcg acatttcgag
       61 acgattttgg cgaagggaag ggagcggcaa tgcgatctag tcaacgaatt cgacgaggtg
      121 cattttgtgt cggtctggcg atgctcgctg gagccggtgg ggcgcttttg agtgccggct
      181 cgcccccgtc atcaaacacc caaggcaaag tggatcggag cgacttgatc gttcacgaat
      241 ggggaacgtt tctgggcatg agcggctccg acggagcgtc ccttgacggc atgtatcacg
      301 aggagcatgc actgccggcg tttgtgcata gccgcagccg cgaccagctc cggctgccgt
      361 ctgttttcct caagggcgaa acgccggtga tttacttcta caccgagcaa cgccagcgtg
      421 tccgggtcgg cgtgggcttt ccgcacggaa tctggacaca atggttcccg caggcgctcc
      481 tggtcaatcc acctctggcc cagcatgccg agacacccga tcgattgagg gacggccgaa
      541 tttgctggca ggccgaagtg atccccgcgt ccgcggtcaa gcccgccact ggccagagat
      601 tggaaaacga gcaacgtccc cagatcgagt tgccggcgac aagtagcgac gccttgtgga
      661 attacgctcg agacgtcgac gcagcatacg tcacaacaag ggacgcaacc aaggagaaaa
      721 cacccgccga gtatgagaag tttctgtttt accgtgggct gggtgaggct cggctgccac
      781 tccgcttcga cgcgagtagc aacggcacgc tgacgctcga acatgagatc atgctggatg
      841 aaggagtccg gcacgcgttt gtcatccgag tcgagaacgg ccgggccgct tatcgttact
      901 tcccagccgt tcgtcccggc gagcagattc acaacgcgat cccatcgatg actcaggcac
      961 ggccgttggc ggagttcacc agagcgatca gcgatgaact ggctgccggc ctgatcaaat
     1021 cgggccttta cgccaaggaa gcccgtgcca tggtcaatac ctggacagcg agctacttcc
     1081 agaccgaggg agttcgcgtg ctgttcgtgt tgccgcaatc ctggaccgac gccttcgttc
     1141 ccatgacagt ttcgcctgcg cctcagcaaa tcgttcgagt catggtcgga cggctggagc
     1201 ttctgacagc cgaccgagaa aagctggccg aaggggccgt gcgcaacctc accggaggcg
     1261 acgccggcaa gcgtcaagac gcttttcgct tcctgcatga acagggacga tacgtcgagc
     1321 cgatcgtccg tcgcgtgatg aagaccacaa ccgatgacgc ggtacggaca gtttgccgcc
     1381 ggctgctcct caccgatttc ctgaccgagc tgcgagcggc tgttcacaac gcggccgatg
     1441 ggagccggct cacgaccgat ccgctcattc tccgcgccca cctggccagg ctactgcggg
     1501 aggtcggttc cgatactgag gcacgcgacc tgggcaccgc catcctggca gatctaaagc
     1561 gatacccact ggctcccaat caggcacttg ccgacaaccc ggcggccctt gaaatccggg
     1621 ccgcggccat cgaggcaatg ggcgacgacc gcaaggcggc cgcagtctac gcccgccgga
     1681 tcgaattgca gattcggtca tacaaaggtg ggctcaatca cggcatgatc cccgggctcc
     1741 gcgactggtg ggtcggccgt gcctacggcc agtgcgtgcg tcgctcgtcg cagaccgcgt
     1801 cgacaaccgc cgcgctcgaa aagcacttga gtgggcaccc ctcaaccgac cgctccgatg
     1861 atttgatgcc tcgcgtattc ctggcatacg tccttgacgg ccagggaaga cccgaacgtg
     1921 cggacgaaga gtggcgagcg ctggcggccg agcccaagcc ggatcaggcc gcagccgcgc
     1981 cggctctcca acccaaagag cccggccaaa ccggaacatg atttacccgg agacgaacgc
     2041 cgcgcacgtg tatgatgaga tggtcggcca ccgggaatcc gcgtttccgc ggccaagagt
     2101 accggaaatc cgaattcccg agccgccctc gcactcgtct gagcgggatc gataagcatg
     2161 tctgcagcca aagacttgac ggatccggta ctcggggcgc gcgaaaccgt cgacctcgaa
     2221 cggtttcgcg cctacctgat cctgctggcg cggctcgaga tcgctccccg tttgcgtgac
     2281 aaggtcgacc tctcgggagt cgtccagcaa acgcttctcg aggcccatca aggcttgatc
     2341 aactatccag gtcgaccgaa aaccgaggcg gagatcactg cctggctgcg ttcgatcttg
     2401 agccataacc ttgcggatgt gttgcgcaag ctgacgactc gcaagcgcga tgtccgccgc
     2461 gaatggtcgc tcgaacaggc actggaccaa tcgacatcgc gtctggaaca gtggctcacc
     2521 gtcgatcaat cgtcgcccag ccagcgtgtc atgcgccagg aagagattct gcggatggcc
     2581 gcaactctgg ccactctccc cgaaggccag cgccgcgcga tcgagttgca tcatctgcag
     2641 ggctggccgc tggccgagat cgccgccgag ctaggtacga cgaaggcggc cgtcgcgggc
     2701 ttacttcatc gcgggctcaa gaacctgcgg atgcagctcg atccgcgaca ataaccttct
     2761 caacgatgaa cgtttcacat cggagatgcc ggcatggcga gcattcccgc aaattcatcc
     2821 gactggagcc aggagcacgc cggcgagcaa gagcagacgc tcgatcaggt catcgccgat
     2881 tatctcgagg cacatgaggc gggtcagccc acgaatcggg atgtcctctt gaagcagtac
     2941 cctgcactgg cagccgagct cgcgcacttc ttcgagaatc aggatcgcat ggcccggcta
     3001 accgcacaaa ttcgcgctgg caatcaaccc ggctgttcac gcggtctgga atccatattc
     3061 attccgtttc ccggaccgga ccacgaaaac tctcaacaac ccgacccatt atcctcaaat
     3121 gaggctcgtg ttcgttattt cggcgattac gaactgatcg agatcctggc gcaaggcggt
     3181 atgggggtcg tctacaaagc ccggcaagtg agtttgaatc gtgtcctggc gctcaagatg
     3241 gtcggtgcgg gccggttcgc gacgcccggc gatctccagc gattccgcct cgaagccgaa
     3301 gcggccgcgc atctggatca tcctcatatc gtgccgatct acgagaccgg tgagcacgac
     3361 ggccatcact atttcagtat gaagctcgtc gacggcggca atttggccgc acaggtcgag
     3421 cgttacggcc acaaccctcg cgccgccgcc aggctggtcg cgacggttgc gcgcgccgtg
     3481 cactacgcac acgaacgcgg catcctccat cgcgacttga agccctccaa tatcctgctg
     3541 ggcggccctg ccggtgcccc gctggaagag cgcgtgcccc tggtcacgga cttcggactg
     3601 gccaaacggg tcgaggcacg gcacaaccct gaattgactc aatcaggctc gatcgtgggc
     3661 acacccggct acatggcacc cgagcaggcc gagggacatc gcgaggcgat cacgacctca
     3721 gtcgatattc acgcattggg ggcaatcctc tatgagctct tgaccggccg gccgccgttt
     3781 cgcgccgaga cgatgctgga aaccctgcga atggtccgcg agcaagagcc cgtgcgtccc
     3841 cgaactctca agccgagaat cgaccgcgat cttgagacga tcgtgctgaa atgcctggag
     3901 aaagccccga accagcgcta tcactccgcg gaagccctcg ccgaagatct cgaacgctgg
     3961 cttggcgatt taccaattcg cgcccgtccc acgacgcttc cccagcgcgc gatcaagtgg
     4021 gcacgacgtc ggccggcggc ggccggactg gtgctgatgg caaccattgc cagcggcatc
     4081 gccattggcg gcgtcatcgc gctacgggcc gagaaagaga aacatcgcaa atctgaggcg
     4141 gagctagtcg agtcgcgcgc tcgcaagatt cagatggaag aggatcgata tttccagcag
     4201 gtgattgcgg ccgaccacgc ttttgaggca aaggacccgg ccacggccca gcggcttttg
     4261 gccgattgcc cgcccttgct tcggaactgg gaatggtatc acttgagccg gcgactccat
     4321 tccgagctac taacgattgt gggacattcg ggattcgttt gccccgactt ccggcccgac
     4381 acgccagacg cggaatgccg caccgacgca ttgagcggct cgctgtggga cgctacggac
     4441 ggtccgaaac ttcgccgaat gcatggccct gacggcactg cgtacgggct ggcgatcgat
     4501 cgggcgggac tacgcttggc cacggcggga tccgatggtc aggtaaagat ctgggacatg
     4561 acccgagtgc gcttgctcca tgtcttccgc gctcatgaag gttggaccgc cggtatcgct
     4621 ttcagcgctg acggcaccag gctcgccttg gccggacagg atcaaacgat ccggatctgg
     4681 gacgtcagct cgcaccctga cgcggccggg gatagagatg gagccttgca ggtccttcgc
     4741 ggccatactg gcggagtttt tggcgtcgcc ttcagtccag atggaaccaa gctggcctcg
     4801 gcgagcaaag acgggaccgc ccgtgtctgg gacttgaccc agcaagaggc gcgaatagcg
     4861 ctcgtttttc gaggtcacga tcaggaggtg tgctgtctcg catttcaccc cgacggaacg
     4921 cggatcgcct caggcggggc ggatcggaag gttcgcattt gggacgccgc caccggccgt
     4981 cagattctcg aatttcacgc ggcgacgagt cgcgtgaacg cgatcgcgtt cagttcggat
     5041 ggtaccaaga tcgcgacggg tagcctcgat cagcccgttg atatctggga cgccggcaac
     5101 ggtcacctga tctctgttct cgcaggccac acgaacccgg ttttcgcggt tgcgttcagc
     5161 tcggatggaa ccagactgat ctcggcgagc caggacgcga ccatcaaact ctgggacctg
     5221 acgtcgaggc cgggagtgag tcgtttcgag ctcgagcgag aagaggcaag ccattccggc
     5281 gataccgttt caaccgatcc gacgatgccg accgtgcgct gggtcggagg cgtggcgttt
     5341 cgaccgtcgg gcgatgagtt tgcggccgca ggatcgaacc agacgatcgg aatctgggac
     5401 gcctccaccg gccggctgaa gcgcaagctt catgacggca atgcaacatt tttcgcgatg
     5461 gcctacagcc gcgatggcgc gcggctggcc gcggccagga cagatcgcga cgttcggatc
     5521 tgggacctga acgcgagccg gcctccgctg gtgctctcgg atgcgcgcga ggggctggcc
     5581 agtgtcgcgt ggagccccga tggaaatgtc atcgccacag gaggagga
//
LOCUS       DATAQL010001196         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2719102, whole genome shotgun sequence.
ACCESSION   DATAQL010001196 DATAQL010000000
VERSION     DATAQL010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719102"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VHS97_20600"
     CDS             <1..212
                     /locus_tag="VHS97_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450667.1"
                     /translation="MGNTPIRAVSIVDSLLPRLEYVAGTSSGPEGTTFTTAVNRVGAT
                     ELNWVLPGVLAPGATGYVSFQAIVR"
     gene            424..2352
                     /locus_tag="VHS97_20605"
     CDS             424..2352
                     /locus_tag="VHS97_20605"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3450668.1"
                     /translation="MALKVAKTLPPGAHARIGQAPSYLRWAIPFLSLLALVAAVSIWL
                     LGPRDANLVRRPGQIAPDFQLVDVRTGQLNRLSDHRGEMVVVVFVGTSCPVGDLYLPR
                     LIELAGLYESRLVNFIAINSNDSESIDEIAEHARRSRVTFPVLKDTENRVADRLLAER
                     TCEALVIDAAGRLRYRGAIDDQYGLGSRRDSASHDYLKDAIDAVLAGRAVSPDLTHVV
                     GCPIERTIPKTASQPATRRVSQPKAASPPAERAPKSPAPVARSPVTYAADVASILHSK
                     CATCHRPGQVAPFSLLTFEQARRWSTSIAEVIADGRMPPWHADPKYGHFSNTRGLTDN
                     ERSLLTAWVEQGAPPGNLSQAPPLPKYPQGWSIGTPDLVFELPESFTIPAEGTLPIQH
                     FRMKTELKEDIWIQAAEARPSDRAVVHHIFVYMETYREGLTRTHKNKVFLTAYTPGDV
                     PSIFPPGVAKKIPAGADLAFEIHYTPLGKVRHDRTSVGVIISKQPPSHLAMTRGLPQR
                     NLSIPPGAADHVEYSDWKTRNDVQILSLMPHMHLRGKSFTYTAHYPDGRSETLLSVPN
                     YDFNWQNVYRLAEPKHIPKGTTILCEAHYDNSAANLANPDPTRTVEWGEQTWDEMLIG
                     YLDYIENQSIQPGSRATK"
     gene            2477..>3197
                     /locus_tag="VHS97_20610"
     CDS             2477..>3197
                     /locus_tag="VHS97_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450669.1"
                     /translation="MLRVLGSPKQFCDGLTRRELLQVGSLGFLGLELPQGTAVAREQK
                     AAASGRLPGFGQAKSCILLFMYGSPSQLETFDPKPDAPLEVRGELGCIPSSVVGLNIC
                     ERLPRLAQVMDKVSLIRSVSHPYPIHGVAFATTGIPRIDLSMEINPRDPKHWPFIGSV
                     VDHVDSRHSPANRQGSSSVPRNLVLPWAFSSQRVGEVARAGPYGGFLGQAYDPTCTEF
                     VGRGTTNARKTLAGKVWEDAEP"
BASE COUNT          597 a         1073 c          909 g          618 t
ORIGIN      
        1 acatgggcaa cacgccgatc cgagccgtct cgatcgtcga cagcctgttg ccgcgcctcg
       61 agtacgtcgc ggggacctcg agcgggcccg aaggaaccac cttcacaacc gccgtcaacc
      121 gcgtcggggc gaccgagctc aactgggtgc tccccggcgt tctcgctccg ggtgccacgg
      181 gttatgtgtc gttccaggcc attgtacggt aaaacgtttc ggcaggaggc caacctctcg
      241 ctatcacgca ggactccact gcccgatcgc gcacgtggcg gccggcaagc aaatttctcg
      301 attctaccgg gtatccttgc tgtgtgtggt tccggaggtg ttcacgtgca accgcagtcc
      361 tcgcgatggg ccgcgcgact tgttctatcg cgacaacgtc gacgaatgag gcgcctgttt
      421 ggaatggctc tgaaagtcgc gaagactctt ccgcccggag cgcacgcccg aatcggccag
      481 gcgccgagct acctccgctg ggctattccg ttcctgtcgc tgctcgcact ggtcgctgcc
      541 gtctcaatct ggctcttggg cccgcgcgac gcgaatctcg tgcggaggcc aggccagatc
      601 gcacccgact tccagctcgt cgatgtcaga accggtcaac tcaaccgcct ctcggatcac
      661 cggggcgaga tggtggtcgt tgtgttcgtc ggcacgagct gtcccgtcgg cgatctctac
      721 ttgcctcgac tgatcgagct ggcaggactt tatgagtctc ggttggtcaa cttcattgcg
      781 atcaactcta acgacagcga atcgatcgac gagatcgccg agcatgcgcg gcgatctcgg
      841 gtgacttttc ccgtcttgaa agacaccgag aatcgcgttg ccgatcggct actcgcggaa
      901 cggacctgcg aggccttggt gatcgacgcc gccggtcgac tccgctaccg aggcgcgatt
      961 gacgaccagt acggcctggg ttcgcggcgt gatagtgcgt cccatgatta tctcaaggat
     1021 gcgatcgacg cggtcctcgc cgggcgcgct gtctcgcccg acctcacaca cgtcgtcggc
     1081 tgcccgatcg agcgcacgat tccaaaaacc gccagccaac ccgcgacccg ccgcgtttcg
     1141 cagcctaagg cggccagccc gccggctgag cgcgctccta aatcgccggc gcccgtggct
     1201 cgctctccag taacctatgc cgccgacgtc gcctcgattc ttcatagcaa gtgcgcgacg
     1261 tgccaccgtc ccggccaggt cgctcctttt tcgcttctga cgtttgagca ggcgcggcgg
     1321 tggtcgacct cgatcgccga ggtgatcgcc gatggacgca tgcctccctg gcacgccgat
     1381 cccaaatacg gccacttttc caacacccgc ggcttgacgg ataacgagcg atcgttgctc
     1441 accgcctggg tcgagcaggg ggcgccgccg ggcaaccttt cccaggcgcc gcccctgcca
     1501 aaatatcccc agggctggtc gatcggcacg cccgacctcg tattcgagct ccccgagtcg
     1561 ttcacgatcc ccgccgaagg gacgctgccg atccagcatt tccgcatgaa aaccgagctc
     1621 aaagaagata tctggatcca agctgccgaa gcgcgcccca gtgacagggc cgttgttcat
     1681 cacatctttg tgtacatgga aacctatcga gagggcctga cgcggacgca caagaacaag
     1741 gtcttcctga cggcgtacac tcccggcgac gtgccgtcga tctttccgcc cggagtcgcc
     1801 aagaagatcc cagccggggc tgatctggcc ttcgagattc attacacgcc gctcggcaag
     1861 gttcgccacg accgaacgtc cgttggcgtc atcatctcga agcaacctcc ttcccacctg
     1921 gcgatgactc gcggactccc gcagcgaaat ctgtcgattc caccgggagc cgccgatcac
     1981 gtggaatact ccgactggaa aactcgcaac gacgtgcaaa ttctgagcct catgccgcac
     2041 atgcacttgc gaggcaagag cttcacctac accgcccatt accccgacgg ccggagcgag
     2101 accctcttgt cggtgcccaa ttacgatttc aactggcaaa acgtctatcg ccttgccgag
     2161 cccaagcaca tccccaaggg caccacaatc ctgtgcgagg cccactacga taattccgcg
     2221 gcgaacctcg ccaatcccga cccgacgcgc accgtcgaat ggggagagca aacatgggac
     2281 gaaatgctga tcggctatct cgactatatc gaaaaccagt ccatccagcc gggttcccgg
     2341 gcgacgaaat gacggccgct gacaagccag acgacaatcc ccgtaaactc tcctttgttg
     2401 aataattctc acatcgaggt tacgttcttc tagtgtctgt gcaggtgatc gacctcgggg
     2461 attcgctgga gcgccgatgc tacgcgtcct gggcagccca aaacagtttt gcgatggcct
     2521 tacgcggcgc gagcttctgc aggtcggctc gcttggcttt ttggggcttg agttgcccca
     2581 gggaacggcc gttgcgcgcg aacagaaggc ggctgccagc ggcaggctgc cgggattcgg
     2641 tcaggccaaa tcgtgcattc tcctgtttat gtacggctcg cccagccagc tagagacatt
     2701 cgaccccaag ccagacgccc cccttgaggt tcggggcgag ctcggctgta tcccgtcgag
     2761 tgtcgtcggt ttgaacatct gcgaacgcct gccccggctc gcgcaggtca tggacaaggt
     2821 ttcgctgatc cggtcggtct cgcatcccta cccgatacat ggtgtcgctt ttgcgaccac
     2881 agggattccc cgcatcgacc tttccatgga aatcaatccg cgcgacccca aacactggcc
     2941 gttcatcggc tcggtggtcg accacgttga cagccgccat tcgccagcga atcgccaagg
     3001 cagttcttcc gttccacgca acctcgtgct cccctgggcg ttcagcagcc agcgcgtcgg
     3061 tgaagttgcc cgtgctggtc cctatggcgg tttcctcggc caagcctacg acccgacctg
     3121 caccgagttt gtgggacgtg gaaccacaaa tgcccggaaa acgctggccg gaaaggtctg
     3181 ggaagacgcc gagccct
//
LOCUS       DATAQL010001197         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2107019, whole genome shotgun sequence.
ACCESSION   DATAQL010001197 DATAQL010000000
VERSION     DATAQL010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107019"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VHS97_20615"
     CDS             <1..510
                     /locus_tag="VHS97_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450670.1"
                     /translation="QPVSKDPTGTMPVLQKPAEPACFKAKFALHCPVGLWNAPNLIQD
                     LPDLNTWAEVATGLELDSQDQVQSLDTRAWHERSERLSRLSAPPPEPTQQLDPILFGS
                     DPTARGEALAAIGLTARSQIPFAEAIHVRPLNSSALVGRARFFAARSEPDKAAALAVL
                     AVSAPPGFV"
     gene            527..1021
                     /locus_tag="VHS97_20620"
     CDS             527..1021
                     /locus_tag="VHS97_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450671.1"
                     /translation="MLERFAATDDAVIAERTAKACLLMRAGPLELNQACQLAERAVEM
                     AYPELVKHAELARGLAEYRRGRLAESIRWMDRSLAWESRRWSIEVPAHLVQAMAHLRL
                     ANITAARAARAKAAAVAKEQMPKIGRDAVGPEWHEWAICTVLQREADALFLDLDFPVD
                     PFGR"
     gene            complement(1845..2492)
                     /locus_tag="VHS97_20625"
     CDS             complement(1845..2492)
                     /locus_tag="VHS97_20625"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX3450672.1"
                     /translation="MFHTNRRGYEHFESKKKSERTFERFSRLLDSHLLHKTAMNTQQR
                     STVVGVFEDRMHASQAITELHEAGFTQKQIGVVMKHSEDSIEIPDRATEHASQTASGA
                     LTGSLAGLGLGALTGLGVLAGVIPVVGPAIAAGTLGVILSNAAAGAGVVGLVGALVGA
                     GLPEHEAKYYQGELEAGRSIITVNAADRADEATSILRRCGGYDISSRSAASARSL"
     gene            2824..5016
                     /locus_tag="VHS97_20630"
     CDS             2824..5016
                     /locus_tag="VHS97_20630"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3450673.1"
                     /translation="MARDKHRPDSAAGPPPGVDAASTKGKAVVTPGRVLVVTSVMLTF
                     ISAWRVAAIVLCDLAGTAYYIGGIVESQIGKSAPWFILAVMLFSYAVRSVYIESCSMF
                     VRGGVYRVVKEAMGGMLAKISVSALLFDYVLTGPISGVSAGQYIVGLANEVLGHYRVR
                     FAIPTAWGSVAIAAAITLYFWKANVRGIRESSDKALKIMAATTVMGVLMIAWCLLTIA
                     VRPETRHLPPAVPDLSNKVDVEGRPIINEVTGKQQDPLGWLAGAAVAEKLRDPSKVNW
                     FSLIGMLGILIAFGHSVLAMSGEETLAQVYREVESPKLPNFKKAAFIVFLYSLLLTSL
                     ISFFAVMIIPDGPRIETYQDNLIGGLAMSVIGPKPARLALHALVVFVGFLILAGSVNT
                     AIVGSNGVLNRVAEDGVLPGWFLKPHPRYGTTSRLLSIIAGLQLFTIVVSRGDVIVLG
                     EAYAFGVVWSFVFKSLAMLVLRFRRPGHREFEVPLNVRIGRYDVPVGLALIFLVLAEA
                     AVVNLFTKTVATVSGGIFAAVLFGIFVATEWYYGRGEHVDHMEEFNEETADRFTPKAL
                     GLSKPERKIIAVQSRNSLQALQRALDETDPENTDVVVVATKVVPRRAAAPGPGLTNAD
                     RALLTNVVKLAEEAGKTVRPLVLPTDDPATTLARIARAIGARELWMGPGRWRRPEKLI
                     DRIARAWSAAAAGEGNSTPLTVRIVGEGQDVRRELTTMTDRPGRVSGG"
BASE COUNT         1066 a         1743 c         1710 g         1101 t
ORIGIN      
        1 cagcccgtgt ctaaagatcc cacgggcaca atgcccgtgc tacaaaagcc cgccgagccc
       61 gcttgcttca aagctaagtt cgctctccac tgcccggtgg ggctctggaa tgccccgaat
      121 ctcattcaag atctgcctga cctgaatacc tgggctgagg tcgcgactgg cctcgagctc
      181 gatagccagg atcaggtcca gtcgctcgac acgcgggcgt ggcacgagcg cagcgagcgt
      241 ttatcgcgac tgagcgcccc gccgcccgaa ccgacgcagc aactcgaccc aatcctcttc
      301 gggtccgacc caacggcgcg cggcgaagct ctcgcggcga tcggcctcac agctcgctcc
      361 cagatcccct tcgccgaagc catccacgtc cgtccgctca attcgtcggc attggtcggc
      421 cgcgcccgct tcttcgccgc acgatccgag ccggacaagg ctgccgctct cgccgttctg
      481 gccgtatctg cgccacctgg atttgtataa ccggcttcgc cgccggatgc tggagcgctt
      541 cgccgcgacg gatgacgctg taatcgccga gcggaccgca aaggcttgcc tgctgatgcg
      601 ggcgggccca ttggagctga accaggcatg ccaattggcc gagcgtgccg tcgagatggc
      661 gtatcctgag cttgtcaagc atgccgagct cgcccgcgga ttagccgagt accgtcgcgg
      721 ccgcctggca gaatcgatcc gatggatgga ccggtcgctc gcctgggaat cgcggaggtg
      781 gtcgatcgag gtgccggcac acctagtgca ggccatggcg catctccggc tggccaatat
      841 aacggctgcg cgcgcggccc gggccaaggc cgcggcagtc gcaaaagaac agatgcccaa
      901 gatcggccgc gacgccgttg gcccggaatg gcacgaatgg gccatctgca ccgttctcca
      961 acgcgaggct gacgctctgt tcctcgattt ggactttccg gtcgatccgt tcggccgctg
     1021 agtgcagctc tatatgactg cgaagacagg tgcgagacgt gcgtacgagg agaggacaag
     1081 tgcgaatcat cgcatcggcg actacgagca caggctgcag actgcgatga cagaaagccg
     1141 cgagcacagg catccagact gcagattgct gcagactgcg agaactagca tgggactgcg
     1201 aggaccgcga ggcatcgcat cggcaagcct atccactatg cccactgagc tcaataggtc
     1261 gggagggaaa ccgagggaaa ccgggccgca atttgttttt ccatcgttct gaccgcttcg
     1321 gagaatccgc gtaattagct tgtcgactcg gtctccgatt ggctcgcttt ccgtgggcgg
     1381 ccttccggac gaacggtccg ctccaggccg agatctctta ccgtttcccg cacccacttc
     1441 tcttcgccgt gtggtcgtgt cgtcgtctct cgatgctcac ccgcgctcgg tctaattccc
     1501 tcgtggtaag gagcgtcgac gcgagccgtc cagttcgccg gtcgttccac gggccaaggc
     1561 tagaacgcaa tacgacagga tacgaattgg ctgccgcagg cgcgcctcaa ccatgacgcg
     1621 ctcgaacgcc tcaaagtcgg actcgttgcg gaacacatgc attctgccga cggatcggtt
     1681 caggacgtga tagacaatcc cctggatccg actctcgctg ttcgtggcac ggcaacatct
     1741 gttcacagaa cgaaagactt ccgtcaatac taatcgcgtc ctggtttccg tcccaacaaa
     1801 aaaggggacg ctccatcatc ccctcagtgc tggtcgggat tgatttacag cgagcgggct
     1861 gaagcggcag atcggctact tatgtcataa ccaccgcacc gacgcaggat agacgttgcc
     1921 tcatcggcgc ggtctgctgc attgaccgtg atgattgaac gcccagcttc gagttcaccc
     1981 tggtagtact tagcttcatg ttccggcagg ccagctccca ccaaagcgcc gacaagccca
     2041 acgacgcccg cccctgccgc agcgttggaa aggatgactc ccaaagttcc cgccgcgatg
     2101 gcgggaccaa cgacagggat cactcccgcc aggacgccca gcccagtaag ggcacccaga
     2161 ccgagaccgg ccaatgaacc ggtcagagca cccgacgcgg tttgcgaagc gtgctcggta
     2221 gccctgtctg gtatctctat tgaatcttcg gaatgcttca taacaacgcc gatttgcttt
     2281 tgagtaaatc cggcttcgtg aagctcagtg atagcctggc tggcgtgcat tcgatcctcg
     2341 aagacgccga cgactgtgga acgttgttgc gtattcatgg ccgtcttatg cagcaaatgt
     2401 gagtccaaca aacggctaaa tcgttcgaaa gtcctttcag attttttttt gctctcaaag
     2461 tgctcatatc cgcgacggtt cgtgtggaac atgtcctccg ctcggcgtgg cgactgcggt
     2521 gatggcctag gttccgacgt aattcccgct cggtcgctct agcctggacg gtgaggtttt
     2581 cgtcggataa catcaacaaa tatcagtgtt tccggaccgc cctcccggac gggcgattcg
     2641 gggcgaaccc attggcgggc aagttgcgac gcgcgaccgt ctggtcccca actcattgat
     2701 ctggtcgtca tcgagggatc ccatcgggcg cccaccgtca tctatagagc aacgagcacg
     2761 aacggccggg cagacctggg tgttagggcc atacggccat tccgttcccg cgcaggacgt
     2821 cccatggctc gagataaaca tcgcccggat tcggccgccg gtcctccgcc aggtgtcgac
     2881 gccgcgtcga ctaaaggtaa ggcggtcgtg accccggggc gggtgctggt cgtcacgagt
     2941 gtgatgctta ctttcatctc ggcgtggcgt gtcgcagcga tcgtcctgtg tgatctggcg
     3001 ggcacggcct attacatcgg cggcatcgtc gagtcgcaga tcggcaagtc ggccccatgg
     3061 ttcatcctgg cggtcatgct gttcagctac gcggtgcgga gcgtctacat cgagagctgc
     3121 tcgatgttcg tccgcggtgg cgtctatcgg gtcgtgaaag aggcgatggg cgggatgctc
     3181 gccaagatat cggtctcggc cctcttattt gactacgttc tgaccggtcc gatcagcggc
     3241 gtctctgccg gacaatacat cgtcggcctg gccaacgagg tacttggcca ttaccgcgtg
     3301 cgcttcgcga ttcccaccgc gtggggctca gtggcgatcg ccgcggcgat caccctgtat
     3361 ttctggaagg cgaacgtccg gggtatccgc gagtccagcg acaaggccct gaagatcatg
     3421 gcggcgacca cggtgatggg cgttttgatg atcgcctggt gtctcctgac cattgccgtc
     3481 cgccccgaga cccgccacct gcccccggcc gttcccgacc tttccaacaa ggtggacgtg
     3541 gaggggagac cgatcattaa cgaggtcacc ggcaagcagc aggatcccct cggctggctc
     3601 gccggagccg cggtggccga gaagctgcgc gacccctcga aggtgaattg gttcagctta
     3661 atcgggatgc tcggcatcct gatcgcgttc ggccactcgg tgctcgcgat gagcggcgaa
     3721 gagacactgg cccaggtcta ccgcgaggtc gagagtccca agctgcctaa cttcaagaag
     3781 gcagcattca tcgttttctt gtacagcctg ttgctaacct cgctgatcag cttcttcgcc
     3841 gtgatgatca ttcccgacgg gcctcggatc gagacctacc aggacaacct gatcggcggc
     3901 ctcgcgatga gcgttatcgg gccgaaaccg gcgaggttgg ccctccacgc cttggtcgtc
     3961 ttcgtggggt tcttaattct ggccggctca gtcaacacgg ccatcgtggg gtcgaacggg
     4021 gtcctaaacc gggtcgcgga agatggcgtg ttgcccgggt ggttcctcaa accccacccc
     4081 cgctacggca caacgtcgcg cctcctgagc atcatagcgg ggctccagct cttcacgatc
     4141 gtcgtgagtc ggggggacgt catcgtttta ggcgaggcat acgctttcgg cgtggtctgg
     4201 agcttcgtgt tcaagtcatt ggccatgttg gtgctcaggt tccggaggcc cggacatcgc
     4261 gaattcgaag tgccgctcaa cgtccgaatc ggccgctacg acgtgccggt cggacttgcc
     4321 ctcatcttct tggtgctcgc ggaggcggcg gttgtgaacc tcttcacgaa gacggtcgcg
     4381 acggtcagcg gcggaatctt cgcggcggtc ttgttcggaa tctttgtggc gacggagtgg
     4441 tattacggtc gcggggaaca cgtcgatcac atggaagagt tcaacgaaga gacggccgac
     4501 cggttcacgc ccaaggcact gggcttgagc aagcccgaac ggaagattat cgcagtgcaa
     4561 tcgcggaaca gcctgcaggc actccaaagg gccctcgacg aaaccgaccc agagaacacc
     4621 gatgtcgtgg tcgtggccac caaagtcgtt cctcgacgtg ccgccgcgcc gggtcccggc
     4681 ttgaccaatg ccgaccgggc gctgctgacg aatgtggtga aattggcgga ggaggccggc
     4741 aagacggtcc ggccgctggt cctgccgacc gatgaccctg cgacgacctt ggcccggatc
     4801 gcccgcgcga tcggcgcacg ggaactttgg atgggacctg gacgctggcg aagaccagag
     4861 aagctgatcg accggatcgc ccgcgcctgg tctgctgccg cggccggcga gggaaattcg
     4921 acacccctga cggtccgcat cgtgggggaa ggtcaagacg tgcggcgaga gctgacgacg
     4981 atgacggatc gccccggtag agtgagtgga gggtgagcac tccacggtaa gcgttgggtc
     5041 atggccgcgc gtcccgctgg atcacaatta cccccgcaac atctcctcgc ccgatacggg
     5101 gcctccgttg cttcacgacg agggtaacag ggacgaacag cggggaaacg acaacgcgat
     5161 tcgtttctcc atcaatttga cggctggatc ggatccgatc cgcgcgatta gcttgtcgcc
     5221 tcagtcgccg attagctagc cttccggaac tgagctcgtg ccacaggtaa gcgaggaagg
     5281 ccgtgtcgac atgcaagcaa caccgtggtg ccattcaagt cgagcgactg ccccatcgtt
     5341 tcagcatctt cgctcaatcg gattgtagga actgcggaca accgttcgtc ccggaattct
     5401 cttgcatggc cacatccgtt cggttcgaat aaggcgacag atcatccggg agtgtcggac
     5461 tggcaggtcg acagctcacc tccgggaatt gtcgtctcag aatacgaccc gatgttgatc
     5521 ctgttcgccg cgagcgaggg agttggccgc tatggtgcca cccaacgagt caaccacgac
     5581 aacggacgcc atatcgggct tcctccaggc gctccgtaag
//
LOCUS       DATAQL010001198         6348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1802379, whole genome shotgun sequence.
ACCESSION   DATAQL010001198 DATAQL010000000
VERSION     DATAQL010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802379"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1663)
                     /locus_tag="VHS97_20635"
     CDS             complement(221..1663)
                     /locus_tag="VHS97_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450674.1"
                     /translation="MSRCELDRRRFLWNAGGGLGGIALAHLLGSEGLLAADGTPSETR
                     GRSRGGLHHPARASRVIELFMSGAASQCDLYDYKPLLIKKHGESFSPGGKVELFQSEP
                     GAVMKSPWSWKQYGECGKWMSNLVPYLAQCVDDMAFLPAMVSKSNVHGPATFLQNTGF
                     VLPGFPSFGAWVSYGLGSLNDNLPTFIVLPDSRGFAPNGPANWSAGFLRASCQGTMVK
                     AASKAPIFDLFPPDDPSLTSASQRDGLALLNEFNRRHKVGREADTRLEARIASYELAA
                     RLQLSAPEVLDLSGESRATKELYGLDDNATADFGRNCLIARRLLERGVRFVQVWSGAD
                     NGFPRRNWDSHEDIARDHGEMGRSMDQPAAALLKDLKARGLLEDTLVIWTTEFGRMPC
                     SQGGKGRDHNPFTFTSWLAGGGIKGGVVHGASDEWSYKAAESPTYGYDLHATVLHLLG
                     IDHTRLTFRHNGIDRRLTDVHGRVIRETLA"
     gene            complement(1677..3818)
                     /locus_tag="VHS97_20640"
     CDS             complement(1677..3818)
                     /locus_tag="VHS97_20640"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX3450675.1"
                     /translation="MRVVIVLGAVTAACLLAGAGTAKGDDLFRTVIAPLLERRCVHCH
                     GDDTPKGSLSITSLGALVRGGKSGPAVEPGEPDESLLIEKISGDKPEMPQKGQPLEKE
                     QVAALRQWVKEGARWPEGVVLRDRRIEDEKWWAIQNLDRPAVPEVKDRSWARTPIDAF
                     ILSRCEAVGLRPAPEADRRTLIRRLTYDLHGLPPTPAEIDAFESDTTPDAYDRLVDRL
                     LASPHYGERWGRHWLDVVHYGDTHGYDKDQRRDHAWPYRDYVIKSLNDDLPYDRFIRE
                     QVAGDVIRPGDSTGVIATGFIAAGPWDLVGHVELREGTVDKLKTRVLDRDDMVTNTMS
                     TFQSVTVHCARCHDHKFDPIPQRDYYRLQAVFADIDRGDRAFAPDKKLVYAVLPVTPR
                     PIHLLRRGEVEQPGDLVQPGSLSCLASLANEFESSQQDHQGSARAALADWIASPRNML
                     TWRSIVNRVWHHHFGRGIVDTPNDFGRNGSKPTHPELLDWLAVGFRDGGKSLKSLHRT
                     IVTSAVYRQSTRDEPAYARIDADNRFLWRMNRTRLDAEELRDSVLAVSGTLDPKMGGP
                     GFELFRYKKDHSPIYDHSAPEAIDNPLVRRRTVYRFTVRSVANPFLECLDSADPNSNT
                     PTRNTTITSLQSLALLNDLFMIRQSQSFARRLENLTADPRHRIEAAFLLALGRRLGSV
                     EVDALETYASKHGLANACRFLLNTNEFAFID"
     gene            complement(4062..5792)
                     /locus_tag="VHS97_20645"
     CDS             complement(4062..5792)
                     /locus_tag="VHS97_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HEX3450676.1"
                     /translation="MGLNQPFGGPLVDLFVSPDRAAEMKATAKDFASLTLDERGLCDL
                     ELLAVGGFSPLKSFLNKTDYERVVGEMRLANGTFWPLPVTLPVAPGDGVAEGKPLALR
                     DVYGNLLAFLHVEEIYAYSKDNEAQHAYGSLDATHPSVAYLKRTPGQYASGTLEVIRV
                     PPHYDFVDLRRTPAELRQHFESLGWSRIVAFQTRNPLHRAHEELTKRAAEQIGGGLLI
                     HPVVGVTKPGDVDHFTRVRCYRALVDNYYEPSAVVLSLLPLAMRMAGPREVLLHAIIR
                     RNYGCTDFIVGRDHAGPGKDSTGKPFYPPYAAQEAMGKFADEIGMGLVDFKQMVYLPD
                     ENRYLGVDEVPAGVKTADISGTDVRDKYLAKGLPLPEWFSRPAVAKILNEANPPRFQQ
                     GLDLWFTGLSGSGKSTIAYATIERLAEYGRNCSLLDGDEIRTHLSKGLGFSKEDRDTN
                     IRRVGYVAGLIAQHGGTTLCAVISPYRAIRDEARKMAKGNFVEVYCSTPLDVCEQRDV
                     KGMYAKARAAVADGKPMGFTGIDDPYEPPLNPEVTLDTSTLSVEECVDRIIDKLLELG
                     YILRHGHVVE"
     gene            complement(5907..6326)
                     /locus_tag="VHS97_20650"
     CDS             complement(5907..6326)
                     /locus_tag="VHS97_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX3450677.1"
                     /translation="MKVSAKAEYACLAVLALARPGPESSPLHIREISEAYGIPERYLV
                     QILLQLKGAGLVASTRGAAGGYRLARAATSISLSEVLVAVEGPDASRRDSSATKRPTT
                     SVLASVWESVRAAERVVLDHTTIAQLAEQSSPHEWII"
BASE COUNT         1202 a         1966 c         1946 g         1234 t
ORIGIN      
        1 acgccgaacc gagccccgac ggctacgcgt cgcgcgtcga ttacgcgccg ggagagcacg
       61 tgtccgtcgt cgtcaatggc gcgacgatcg ggtcagtcgc ggttgccgat atactttctt
      121 gagttaccct cagccgtccc acagccgggt cgccccaggg atttgcgccg cgccgcccgc
      181 ggtccacgga acaaagcccc gcgacgtacg agatcgccgt tcaggccagg gtttctcgaa
      241 ttactcgacc atgaacatcg gttaggcggc ggtcgatgcc gttgtgccgg aatgtcaatc
      301 gcgtgtggtc gatgccaagg agatgtagaa ccgtggcatg gaggtcgtag ccataggttg
      361 ggctctcggc cgctttgtat gaccactcgt cgctggcgcc gtgaacaacg ccccctttga
      421 ttccgccgcc ggctaaccag gaagtgaacg taaagggatt gtggtcgcgc cccttgcccc
      481 cctggctgca gggcatgcgg ccaaactcgg tggtccagat cacgagggtg tcttcgagca
      541 ggccgcgggc cttcaaatcc ttgagcaggg cggccgccgg ctgatccatg ctccggccca
      601 tctcgccgtg atcgcgcgcg atatcttcgt gcgaatccca gttgcggcgg ggaaagccat
      661 tgtcggcgcc gctccagact tgcacgaatc gcacaccccg ttcgagtagg cgtcgggcaa
      721 tcaggcagtt gcggccgaag tcggccgtcg cgttgtcatc aaggccgtaa agttctttgg
      781 tcgcgcgcga ctcgccggaa aggtcgagga cctcgggtgc actcaattgg agccgggccg
      841 ccagctcgta cgacgcgatc cgcgcctcca atcgagtgtc ggcctcgcgt ccgaccttgt
      901 gccgccggtt aaactcgttc aagagcgcca gcccgtcgcg ctggctcgcc gacgtgagcg
      961 acgggtcgtc aggcgggaag agatcgaaga tcggggcctt ggaggcggcc ttgaccatcg
     1021 tcccctggca cgaagctcgc aagaatcccg cgctccaatt tgctgggcca ttgggcgcaa
     1081 acccgcgcga gtcggggagc acgatgaacg tcggtaggtt atcgttcaag cttcccagcc
     1141 cgtaagaaac ccacgcacca aaactgggaa accccggcag tacgaatccc gtgttttgga
     1201 gaaaagtcgc cggaccgtgc acattggact tcgacaccat cgctggcaga aacgccatgt
     1261 cgtcgacgca ctgggcaagg taaggcacca ggttactcat ccactttcca cattcgccat
     1321 actgcttcca gctccatggg ctcttcatca cagcgcccgg ctcgctctgg aacaattcga
     1381 ccttgccccc cgggctgaac gattcaccat gctttttgat caacaatggc ttgtaatcgt
     1441 aaagatcgca ctggctggca gcacccgaca tgaatagctc gatcacccga ctcgcccgcg
     1501 ccgggtgatg cagccctccg cgagaccggc cacgcgtttc ggacggtgtt ccatccgctg
     1561 cgagcaggcc ttccgagcca agcaggtgcg cgagcgcgat acctcccagg ccgcctccgg
     1621 cgttccataa gaagcgacga cgatcaagct cgcagcggct catcccggtt cctcactcag
     1681 tcgataaatg cgaactcatt ggtgttgagc aggaaccggc aggcgttggc cagcccgtgt
     1741 ttggacgcgt aagtttctag cgcatccact tccacggaac cgagccgacg gccaagagcg
     1801 agcaggaatg cggcttcaat ccgatgccgc gggtcggcgg tcagattctc gagccgtcga
     1861 gcgaaagact gcgactggcg aatcatgaac aggtcgttga gcaaagccag cgactggagc
     1921 gaggtgatcg tcgtatttcg ggtcggagtg ttcgaattag ggtcggcact gtcgagacat
     1981 tcgaggaacg ggttcgcaac actccgcaca gtgaatcggt aaaccgtgcg ccgccgcacg
     2041 aggggattgt cgatcgcctc cggtgcggaa tgatcgtaga tcggggaatg atctttcttg
     2101 taacggaaga gctcaaaacc cggcccaccc atctttggat ccaacgtccc gctgaccgcc
     2161 aggacactgt cgcgcagctc ctcggcatca agccgcgtgc gattcattcg ccagaggaag
     2221 cggttatcgg catcgatcct ggcataggcc ggctcgtcac gcgttgattg ccgataaacc
     2281 gcacttgtta caatcgtgcg gtgcagcgac ttgagggatt tgccaccgtc gcggaacccg
     2341 actgccagcc agtccagcaa ttcgggatgg gtcggctttg atccgttgcg gccgaagtcg
     2401 ttgggcgtat cgacgattcc gcgtccaaag tgatggtgcc acacccggtt gacgatcgat
     2461 cgccaagtaa gcatattcct agggctggcg atccagtcgg ccagggccgc gcgagcactg
     2521 ccttgatgat cctgctgaga cgactcgaat tcgtttgcca gactggcgag gcacgagaga
     2581 cttccaggtt gcaccaggtc gccgggctgc tcgacctcgc cgcgccgtag taaatggata
     2641 ggccgcggag tgacgggcaa tacggcgtag accagtttct tgtccggcgc gaatgctcga
     2701 tcgcctcgat cgatgtcggc gaacactgcc tgtagccggt aatagtcgcg ctgggggatc
     2761 gggtcgaact tatgatcgtg gcagcgggcg cagtggactg tcacgctctg gaaggtcgac
     2821 atcgtgttgg tcaccatgtc atcgcggtcg agaacccgtg ttttgagctt gtcgaccgtc
     2881 ccttcgcgta gctcgacgtg gccaaccaag tcccatggac cggcggcgat aaagccggtc
     2941 gcgatcaccc cggtcgagtc gcccggccga atgacgtcgc cggccacttg ctcgcggatg
     3001 aatcggtcgt aggggaggtc gtcgttgagc gacttgatga cgtaatcgcg atatggccag
     3061 gcatggtcgc gccgttggtc cttgtcatag ccgtgagtgt caccgtagtg gaccacgtcg
     3121 agccagtgtc ggccccatcg ctcgccgtag tgcggcgacg ccaagagacg atcgacaagc
     3181 cggtcatatg catccggcgt cgtgtcactt tcgaatgcgt cgatctcggc gggtgtcggc
     3241 gggagtccgt ggaggtcata ggtcagccga cggatcaggg tgcggcggtc agcctcaggc
     3301 gcaggtcgca gaccgaccgc ctcgcaccgg ctcagaataa aggcatcgat cggcgtccgg
     3361 gcccaggacc gatccttcac ctccgggact gcgggccgat ccagattctg gatcgcccac
     3421 catttctcgt cctcgatccg gcgatctcgc aggaccactc cctccggcca gcgagcccct
     3481 tctttgaccc actgacgcag ggccgcgacc tgttccttct cgagcggttg tcctttctgc
     3541 ggcatctccg gcttgtcacc cgagatcttc tcgatcaaca aactttcatc tggctctccc
     3601 ggttccaccg caggtccgct cttacctccg cgcacaagcg caccgaggga cgttatcgaa
     3661 aggctcccct tcggcgtgtc gtcgccgtgg caatgcacgc accgtctttc gagaagcggc
     3721 gcgatcaccg tccggaacag atcgtcgcct ttagccgtgc ctgcaccggc caggaggcaa
     3781 gctgccgtga cagccccgag tacgatcacc acgcgcatgg tcataccatt cgttcagctc
     3841 gggaaggttg ttcaatgagg aatcgccaat cgaggattcc aagacgcgca actggcgccg
     3901 gccaggccgc tttgagcacc acatcctatc accgcatccg tggcagggcg cacagattta
     3961 aacaataatc tccaccgaca gcggcaagca accctccgcg gccgcccgac gttggttcgg
     4021 cgattcgccg ccggatcgtg tgtcggccaa tcagaggccg gtcactccac gacgtgcccg
     4081 tgcctgagga tgtagcccag ttccagcaac ttgtcgatga tgcgatcgac gcattcctcg
     4141 actgaaagtg tgctggtgtc gagcgtgact tcggggttga gcgggggctc gtacgggtcg
     4201 tcgatgccgg taaagcccat gggcttgccg tcggccacgg cggcgcgtgc cttggcatac
     4261 atccccttaa cgtcacgctg ctcgcaaaca tcgagcggag tgctgcagta gacttcgaca
     4321 aagttgccct tggccatctt gcgggcttcg tcacggatcg ctcgatacgg actgatgacg
     4381 gcgcaaagcg tcgttccacc gtgctgggcg atcaggccgg cgacgtaacc cacgcgcctg
     4441 atgttggtgt cgcggtcttc cttgctaaag cccaggcctt tggaaaggtg ggtacggatc
     4501 tcatcaccgt ccaagagcga gcaattgcgg ccgtattcgg cgagtcgttc gatcgtcgca
     4561 taagcgatcg tgctcttgcc cgagcccgat aatccggtga accacaaatc caacccttgt
     4621 tgaaacctgg gcgggttggc ttcgttcaag atcttggcga ccgccggccg gctgaaccac
     4681 tcgggcagcg gtaacccctt ggccaggtat ttgtcgcgga catcggtgcc ggagatgtcg
     4741 gccgtcttga cgccggccgg gacctcgtcg actcccaggt aacgattctc gtcgggcagg
     4801 taaaccatct gcttgaagtc gaccagtccc atcccgatct cgtccgcgaa tttacccatg
     4861 gcctcctggg cggcgtaagg aggataaaac ggcttgcctg tcgagtcttt gccgggccct
     4921 gcgtggtcac ggccgacgat gaaatcggtg cagccgtagt tgcggcgaat gatcgcgtgc
     4981 aacaggactt cccgaggacc cgccatgcgc atcgccagcg ggagcaggct gagaacgacc
     5041 gcggaaggct cgtagtagtt gtcaaccagg gctcgatagc agcgtactcg cgtaaaatgg
     5101 tcgacgtcgc ccggcttggt cacgccgacg accgggtgga tcaaaagacc gccgccaatt
     5161 tgctcggccg cccgcttggt cagctcttcg tgggcccggt gcagcggatt gcgggtctgg
     5221 aacgcgacga tgcggctcca gccgagcgat tcaaagtgct ggcgaagttc ggcgggagtg
     5281 cgccgcaggt cgacgaagtc gtagtgcggt gggacgcgaa tgacctcgag tgtgcccgat
     5341 gcatattggc cgggcgtccg tttcaaatag gccaccgacg gatgggtcgc gtcgagcgat
     5401 ccataggcat gctgcgcttc attgtctttg gaataagcgt aaatctcttc aacatgcaaa
     5461 aacgccaaca aattgccgta gacgtcacga agcgccagcg gttttccttc cgcgacgcca
     5521 tcgcccggcg cgaccggcag cgtcaccggc aatggccaga acgttccatt ggccagacgc
     5581 atttcgccca caactcgttc atagtccgtt ttgttcagaa agcttttcag cggcgagaaa
     5641 ccgccaacgg ccagcagctc gaggtcgcac aatccacgct cgtcgagcgt caggctggcg
     5701 aagtctttcg cggtagcctt catttcggcg gctcggtctg ggctgacgaa cagatcgacg
     5761 agtgggccgc cgaatggttg gttcagtccc acggacgccg gcgggatcgt ggacttcgtc
     5821 gctttggtcg atgtcgtgac agtaatgggt ggcctccttt tcaatctcaa gatcatcgtc
     5881 gttagattcc gaccccctct ctgaggtcag atgatccact cgtggggcga ggactgttcg
     5941 gcaagttgag cgatcgtggt gtggtcgaga acgacgcgtt ccgcggcgcg gacgctttcc
     6001 cagacagagg cgagcacgga ggttgtcggc cgcttggtag cggaagaatc ccggcgcgac
     6061 gcatcgggcc cttccacggc aaccaggacc tccgacagcg agatgcttgt ggcagcgcgc
     6121 gccaggcgat agccccccgc cgcacctcga gtgcttgcca ccaaaccggc acctttcaac
     6181 tggagcagga tctgaaccaa gtaccgttcg ggtattccat aagcttcaga aatttcgcgg
     6241 atgtgcagag gagacgactc gggccccggc cgtgccagcg ccagcaccgc cagacacgca
     6301 tattcagcct tcgcagagac cttcatcggg ggcgatcgct ccgtactt
//
LOCUS       DATAQL010001199         8156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3479376, whole genome shotgun sequence.
ACCESSION   DATAQL010001199 DATAQL010000000
VERSION     DATAQL010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3479376"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VHS97_20655"
     CDS             <1..778
                     /locus_tag="VHS97_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450678.1"
                     /translation="EHPDSPVRPEVQRRLDIYLEKLDDRDIEQARDYSRQNSTQFATR
                     IDRYQDYLRAHQAGGRYISEAIEAKDRILREWDTYAYRQAYDHAVSHPDDVAEVARRL
                     REYLRDQPDGRHSADAKHYLDWWDKVTVPGHYRVTLRRGEVEPTAGKYFSGGAPDLGV
                     VIEVAGTVYGPSSVIRNSHRPIWDYTFPQPITWKLGDPVTIRIIDYDWSASEIYALNS
                     QEGDPLAMKILSGTIKPAKGGRTSLVFSSDFSVPTLSTPE"
     gene            969..1694
                     /locus_tag="VHS97_20660"
     CDS             969..1694
                     /locus_tag="VHS97_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450679.1"
                     /translation="MSPLVMIKLPAKIRFEQAVYGSFPFWNRGYSLLCRSQGCRPEWL
                     GEMPAVCQRYGEPPTGVTEADSVFALQLKSGPWMIVGVHPQGLDDHDRPGATAFHALF
                     VGSWAYRWAGADPFVFTVAMRREWSAADQDRRMPTGVCTLGAMTRIASSNPAADIDPR
                     VPLSVSALTEGRRVVVQSLEPIDGLARSVWTRLSHRVRNSTSVATWAFDNANNFDLVA
                     LPKLAGVALNPRDLVIAVEHAGH"
     gene            1867..2385
                     /locus_tag="VHS97_20665"
     CDS             1867..2385
                     /locus_tag="VHS97_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HEX3450680.1"
                     /translation="MTRSLQVRWIDSIRILGLLTIFGTTIGCAGGQVVTPEAIAQAKQ
                     AWTTAGIRDYDLEWTVTGAQNNHYYVTVRDGDVRKVESVQSDGRRFELHPHQTRFYSV
                     DGLFLTIADELALLKTDQPFGQPPGTKVVMRFKPDKKLGYPLWYRRDVMGTALAIAID
                     VVKLVPLASNAN"
     gene            2618..3526
                     /locus_tag="VHS97_20670"
     CDS             2618..3526
                     /locus_tag="VHS97_20670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3450681.1"
                     /translation="MAKSELQNQDRHWSRHAARYDDLFVDPYDPAVESPLRNALEAIP
                     DASNKIVADLGCGTGPLLPYLAERFSRVIALDFAPGMLERARERLNALTRSGEGEPPG
                     EPRHNPARTDFGELSRAEPRPPGMTQAPIGSETVRRVTFLERPMHQLDDLAGQIDVAV
                     AINSLVMPDVRLIDRTLQSIRASLRPGGQFLGVVPSIDTIYYHLMLLMDQSIDQGLET
                     KEAKRLAGLHAERRYYDFAFGEFHFEGLRQKFWQPVELEYRLRKAGFTSTTLSKVLYP
                     WDESLTGNETPVEFSRSWDWFFLVRT"
     gene            3657..4004
                     /locus_tag="VHS97_20675"
     CDS             3657..4004
                     /locus_tag="VHS97_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450682.1"
                     /translation="MPSERQNMSEQDISIKAREHELYVPVLPDAPPVKPFPVYLRETP
                     GEPLSPGTKFVLAIAGIVVALLFVAALWRVTHPRATKPKAGAARPAAKTAVIVDPRAP
                     AFVPPSVSFTMQS"
     gene            4113..5228
                     /locus_tag="VHS97_20680"
     CDS             4113..5228
                     /locus_tag="VHS97_20680"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3450683.1"
                     /translation="MTSSRIALLGLVLSMNPAILPSSAVPCQAGSSDQDDDASSKPKT
                     LTPGRVGSSLLSINEDYARQLLQLERQRLERLAQLAARQTPKEAAATYDQLFRSAITN
                     NLFREAEPAAQHVLKSASEPITQFLARTIDIIASADRGAFDESLSELRAVIESVANPT
                     QPAAVALDTSALLALCEAYYQRLLQGDQFDAARKAFQLLQKESANPAIKGFSARRLAQ
                     LDLIGKPAPAIVGTDLDGKPVNLAELKGNVILVVFWASWCVPNSTEIAALDQVYSAFQ
                     NRGFRVLGINVDAMQTGGPKLETVLPNIRRFLTDHNVRWPNLINGTGTNNYAQAYGVA
                     EIPSNVLIGRDGNVIHLDLSPSKNLESVVSRSVAPAS"
     gene            complement(5488..6237)
                     /locus_tag="VHS97_20685"
     CDS             complement(5488..6237)
                     /locus_tag="VHS97_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450684.1"
                     /translation="MTSKRGGRKILRTLFAAALAFGFGTPRRAEAQWGGAFGGWGWGG
                     FSQVPKPESFLNSKALIDASRDTHIPSRDVYANNPNSYINHIRDNGFVERYDVARREP
                     SHYRYAPAPGQSSPGMTPTAMTASQPMPLLPLASFYDPKGQIVWPADSPVAGDLKEKR
                     TVFDQASQLALAETTKSGVASIATVTDARQKLLEYGRPALQYVRTHETPRIADGFHLF
                     LLSLYQSLAQAVNPTATAAAMPVTPTAPPSS"
     gene            complement(6362..7138)
                     /locus_tag="VHS97_20690"
     CDS             complement(6362..7138)
                     /locus_tag="VHS97_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX3450685.1"
                     /translation="MSTKVAVDVTELWREFKEHPTTDLRNHLVERYLPLVKYNAERIW
                     QRLPEGVDLDDLISAGVFGLMDAIDAFDLSRGVKFETYCVPRIRGAMLDELRTMDWVP
                     RLVRSKATKMEEARKELEARHGRPPTDVELAEQLGIPMEEYEKMASDANAVGLISLNK
                     KWYETDSYKDVREIDILEDKKGEDPTRRLQRKDLMRMVTKGLSRNERLIIILYYYEEL
                     TMKEIGATLDLSESRVSQMHSSIIQRLQSQLSTRRPEFGT"
     gene            complement(7425..>8156)
                     /locus_tag="VHS97_20695"
     CDS             complement(7425..>8156)
                     /locus_tag="VHS97_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX3450686.1"
                     /translation="VLDADIGLANLDLLCGLTPRHDLGDVLAGRCRLVDALVSGPGDI
                     QIVPGAHAIRTGEEDLAGGAARLVAELTELEAESDFVLVDAGSGLGPGVGLLASAAQQ
                     AVIVSTPEPTSIADAHAAICRFRKLAVQPRLRVLLNQTTSTSESVEVLDQFIASTRQF
                     LGAVISPLGTGSIRADPHVPLAVRGRKPFLTAYPGSAASRGVRRLARALLREHHPPGR
                     DMGFFAALVERWPLSRGARSPSQFA"
BASE COUNT         1657 a         2490 c         2382 g         1627 t
ORIGIN      
        1 cgagcatccg gatagtcctg ttcgcccgga ggtgcagcgc cggctggata tctaccttga
       61 aaaactcgat gaccgcgaca tcgagcaggc tcgcgactac tcccggcaaa actccactca
      121 atttgcgacc cgaatcgacc gctaccaaga ttacttgagg gcgcaccagg ccggtggcag
      181 gtacatcagt gaggcgatcg aggccaagga tcgcatcctc cgtgaatggg acacctacgc
      241 ctatcgccag gcctacgacc atgctgtctc gcatcccgac gatgtggccg aggtcgctcg
      301 gcggttgcgc gagtatctcc gcgaccagcc cgatggccgt cactccgccg acgccaagca
      361 ctatctcgac tggtgggaca aggtcaccgt gcccggccat tatcgcgtca ccttgcggcg
      421 aggtgaggtc gagccgaccg ccggcaagta tttttctggc ggagcccccg acctcggcgt
      481 cgtcatcgag gtcgccggaa cggtgtacgg tccatcgtcg gtgatccgca attcgcaccg
      541 gccgatatgg gactacacgt tccctcagcc gatcacctgg aagctcggtg acccggtcac
      601 gatccggatc atcgactatg actggtctgc cagcgagatt tacgcgctta atagtcagga
      661 aggagatcct ctggccatga agatcttgtc tgggaccatt aagccggcca aggggggacg
      721 tacctctctt gtcttttcct ccgacttttc tgtccccacg ttgagcacgc ccgaatgagt
      781 ggaaagttcg gcaaggttca aaagcaggtt actccacagg gggtggctgg ggtcgacgcc
      841 cgcagggcga cgcccccaga tccgaggctt ccgcgccggg ggctcgctgg ctgtcgccag
      901 ctcgacccca gccacccagc tcttgtcacc cgcgaagatg ctaaaattga accatggcgg
      961 aagtttgagt gtctccgctc gtcatgatca agctgccagc caagatccga tttgagcaag
     1021 ccgtatatgg cagcttcccc ttctggaatc gcggctactc gctcctgtgc cgttcacagg
     1081 gatgccgtcc cgaatggctt ggcgagatgc cggcggtgtg ccagaggtac ggcgagccgc
     1141 ccacgggagt gaccgaggcc gacagcgtct ttgccctcca gctcaagagc ggcccttgga
     1201 tgatcgtggg tgtgcatcct caagggcttg atgaccacga tcggccggga gcaaccgctt
     1261 ttcacgcgct tttcgtgggt tcatgggcct accggtgggc cggcgccgac ccgttcgtct
     1321 tcacagtggc gatgcgccga gagtggagcg cggccgacca ggatcgccga atgcccaccg
     1381 gcgtttgcac tctcggagcg atgacgagga tcgcaagctc caaccccgcc gcggatatcg
     1441 acccaagagt tcccttgagt gtgtcggcgc tcaccgaggg ccgtcgggtc gtcgtgcagt
     1501 cactcgagcc gatcgacggt ctggcgcgga gcgtatggac ccgactatcg catcgtgtcc
     1561 ggaacagcac ttcggtcgcg acctgggcct tcgacaacgc caacaacttc gacttggttg
     1621 cactgcccaa gctcgccggg gttgccctca atccaaggga cctggtcatc gccgtcgagc
     1681 acgccggcca ctgacaaccc tgcgggtgcg ccaacggatt ctggcactcc gctggccatg
     1741 acttgataga ccagatgagg gctgttcaac cgaccgggct cctggcattc tggagaaaga
     1801 gcaaattcag ctctcacgcg tactcggcac ccaggccaag ttgggaacag gtgagccttg
     1861 gcaaccatga cccgttcgct gcaagtacgc tggattgata gtatcagaat actcggcctc
     1921 ctgacgatct tcggcaccac gatcgggtgc gcgggcggac aggtggtgac tcccgaggcc
     1981 atcgcccaag ccaagcaagc ctggacgacg gctggcattc gcgattacga cctggaatgg
     2041 accgtcactg gagcgcagaa caatcactac tatgtaaccg tgcgcgatgg tgacgtgcgc
     2101 aaggtcgagt cggttcaaag cgacggccgt cgctttgaac ttcatccgca tcagacgcga
     2161 ttttacagtg tggatggcct gttcctgacc atcgcagacg agttggcgct gttgaagacc
     2221 gatcaaccgt ttggccagcc gccagggacc aaggtcgtga tgcggtttaa gccggataag
     2281 aagttgggtt atccactctg gtaccgccgc gatgtgatgg gcacggcgct ggccatcgcg
     2341 atcgacgtgg tcaagctcgt ccctcttgca tcgaacgcga attgaacgcg tgccagttgc
     2401 cggtcaaagg gatttatcca tgcgcggcag tcggcgcgat cgcgccgaat tatcgcgata
     2461 gatcgccagt cggttgcgag accccccctg aggacaactg gccggatccg cctggttatc
     2521 ctatgctcga gtgaaaatcg gataaaccgc gtcgaggatc atcttggggc gggataagag
     2581 tggccggcgc aggacacgcc gtgttagggc aaagcctttg gccaagagcg aacttcaaaa
     2641 ccaggatcgc cactggagcc ggcacgcggc gcggtacgac gacctgtttg tggatccgta
     2701 cgatcccgcc gtcgagagtc cgttacggaa tgcgctcgag gcaatccctg atgcctcgaa
     2761 caagatcgtg gccgacctcg gttgcggcac cggtccactc ttgccctacc tggctgagcg
     2821 gttcagccgg gtaattgcgc tggacttcgc gccggggatg ctcgagcgcg ctcgtgaacg
     2881 actaaatgcc cttacacgat ccggggaggg cgagcctccc ggcgagccac ggcacaatcc
     2941 ggctcggacg gacttcggcg agctcagtcg agccgagcct cgccctcccg gaatgacgca
     3001 agcccctata ggttctgaaa cggtaagacg agtgactttc cttgagcgtc cgatgcatca
     3061 actcgacgat ctcgccgggc aaatcgacgt tgccgttgcc atcaattcgc tggtcatgcc
     3121 cgacgttcgg ctgatcgacc ggactttgca atccatccgt gccagcctca ggcccggcgg
     3181 tcagtttttg ggtgtcgtcc cttctatcga cacgatttac taccacctga tgctgctcat
     3241 ggaccaatcg atagaccagg ggctcgaaac gaaggaagcc aagcggcttg cgggcctcca
     3301 tgccgagcgc aggtactatg attttgcgtt cggggaattc cacttcgagg gcctgcggca
     3361 aaagttctgg cagccggtcg agctcgagta ccgcctccgc aaggccggat tcacgtccac
     3421 gaccctcagc aaggtccttt atccctggga tgaaagcttg accggcaacg agaccccggt
     3481 cgaattctca cgaagttggg actggttttt cctcgtgcgc acttgatcgc gtacaataag
     3541 agtgaagcga aggcccaaag atcatacgta ctcgcttcat tgccgtaagt cgtggttgag
     3601 ccaaggctga aatttccatc acggtcgctc gcccgacaac ggtgagggaa tagaccatgc
     3661 cttccgaacg acaaaacatg tctgagcagg atatctcgat caaggcacga gagcacgaac
     3721 tctacgtacc agtgcttccc gacgcccccc ccgtcaagcc gttcccggtc tacttgcgcg
     3781 agacgcccgg agagcctctt tcgcccggca ccaaatttgt acttgcgatc gccggtatcg
     3841 tcgtggcctt gctcttcgtg gctgctctgt ggcgggtcac tcatccacgc gctaccaaac
     3901 cgaaggcagg agccgcccgt ccggccgcga agaccgccgt catcgtcgat ccgcgtgcac
     3961 ccgctttcgt gccgccgtcg gtttctttca ccatgcaatc gtagggtgtg ttacccatgg
     4021 catgattgtg gcctcgcgac gcttttccgc gtccggtatc ctatggtctg tatatcgaca
     4081 ccgaatcaca ccgctagagg gttgtatcga cgatgacttc ctcccggatc gctctcttgg
     4141 ggctggtctt gagtatgaat cctgcgatac tgccatcatc ggcggtcccg tgccaggccg
     4201 gctcgtccga tcaagatgac gacgctagct caaagccgaa gacgctgaca ccggggagag
     4261 tcggctcgag cttactgtcg atcaatgaag attatgcgcg ccagctcctc cagctcgagc
     4321 ggcagcggct tgagcggctt gcccaactgg ctgcccgcca gacgcccaaa gaggccgccg
     4381 ctacttatga ccagttgttc cgctcggcga tcaccaataa tctgtttcga gaggccgagc
     4441 cggccgccca gcacgtcttg aaatccgcca gcgagccgat tactcagttt ctggcaagaa
     4501 cgattgacat cattgcctct gccgacaggg gagcgttcga cgagtcactg tccgagttgc
     4561 gcgctgtgat cgaatcagtg gcgaatccga ctcaaccggc ggcggttgcg ctcgatacgt
     4621 ccgcattgct ggcgttgtgt gaagcgtatt atcagcgtct actccaggga gaccagtttg
     4681 acgcggcgcg gaaagccttt cagctattgc agaaggaatc ggcgaacccc gcgatcaagg
     4741 gattcagcgc tcgccgcctt gctcagctcg acctgatcgg caagccagca ccagcgatcg
     4801 tcgggaccga tctcgacggc aaaccggtga accttgccga gttgaaaggc aacgtaatcc
     4861 tggtcgtttt ctgggcaagc tggtgcgtgc ccaactcgac agagattgcc gcactcgatc
     4921 aggtgtatag cgcgtttcag aatcgcgggt ttcgcgtgct cggtatcaac gtcgatgcga
     4981 tgcaaactgg cggcccgaag ctggaaacgg tcttgccaaa tatccgcagg ttcctgaccg
     5041 accataatgt ccgctggcca aacctgatca atggcacggg caccaacaac tacgctcaag
     5101 cctacggcgt tgccgagatt ccgtccaacg tcttgatcgg tcgcgacggc aacgtcattc
     5161 atctcgacct ctcacccagc aaaaacctcg agtcagtcgt gagccgatcg gtcgcaccgg
     5221 cgtcctgagc gaaggtttga ctcgttccca cgcggagcgt gggaacgagt agggaatcat
     5281 ctgcggatcg agtcgttggt tgccgtcaca accgactggt gatccagcgg ttgaagacgc
     5341 ggagcgtcga agacggcatt cccacgcaag agcgtgggaa cgagtgcgaa cgagtactag
     5401 aagaccaccc gtcaatcgaa ggttgacgga acactagccc gtgtccggtg gactcacggg
     5461 caagataaga ttcccttacc agtcatctca ggacgaaggt ggggcagtcg gggtgacagg
     5521 catagcggcc gccgtggccg ttggattcac cgcttgggcc aatgactggt agagggagag
     5581 gagaaagagg tgaaacccat cggcgatccg aggggtttcg tgggtccgga catattgcaa
     5641 ggcgggccgc ccgtattcga gcaatttctg acgagcgtcg gtgactgtgg cgatggatgc
     5701 gacaccgctt ttcgtggtct ccgccaaagc caactggctt gcctgatcga agaccgtccg
     5761 cttttccttg agatcgccgg cgacaggtga atcggccggc catacgatct ggcccttcgg
     5821 gtcgtagaaa ctggccagag gcaacaacgg catcggctga ctagcggtca tcgccgtggg
     5881 agtcatgccc ggactcgact gaccgggggc cggagcatag cggtaatgcg atggctcccg
     5941 ccgcgcaacg tcgtaacgtt cgacgaaacc attgtcgcgt atgtgattga tgtaggaatt
     6001 gggattgttg gcgtaaacgt cccgcgacgg aatatgagta tcgcgggagg catcgatcaa
     6061 cgccttggag tttaaaaaac tctccggttt gggtacctgg ctaaagcctc cccatcccca
     6121 accaccgaag gctccgcccc attgagcttc ggcccgccta ggtgtcccga agccgaacgc
     6181 aagggcggcg gcgaaaagcg tccgtaagat cttccgccca ccacgtttcg atgtcatgac
     6241 cggtaccccg gttggagcta tgccaaggtg caaaccaaac ggacggtcag cgtctcatgc
     6301 ttccaccgcc atctcctact ctcattgtac ggcgcagtcc gccgagacac agcggttttt
     6361 ttcaggtccc gaattcgggt cgtctggtcg aaagctgaga ctgtaatcgc tgaatgatcg
     6421 atgagtgcat ctgcgagact cgcgattcgc taagatcaag cgtcgcgccg atctctttca
     6481 tcgtcagctc ttcatagtaa tacaaaataa taattaggcg ttcattgcga ctgagtccct
     6541 ttgtgaccat gcgcatcagg tctttacgct ggaggcggcg ggtcggatct tcaccttttt
     6601 tgtcttcgag aatatcgatc tcacgcacgt ctttgtagct gtcggtctca taccatttct
     6661 tgttaaggct gatcaggcca acggcgttgg catccgaggc catcttttca tactcttcca
     6721 tgggaatacc gagctgctcg gcgagctcga cgtcggtagg aggccggccg tgccgggctt
     6781 cgagttcctt ccgggcttct tccatcttgg tggccttgga ccgtaccaac cgtggaaccc
     6841 aatccatggt ccgcagctca tcaagcatgg cccctcggat tcgcggtacg caataggtct
     6901 cgaacttgac gccccggctc aaatcaaacg catcgattgc atccatcagg ccaaaaaccc
     6961 ctgcggagat cagatcgtcg agatccactc cttcgggtag gcgctgccag atccgttcgg
     7021 cgttatactt aacgaggggc aagtatcgct cgaccagatg attccgcaaa tccgtggtcg
     7081 ggtgttcctt gaactctcgc cagagttcgg tcacatccac agccaccttt gttgacatcc
     7141 tgacctccat gtcggctagg caagaggcgg cacatcctgt gcaacagggc catagcgtca
     7201 cgtcgctcga tcaacggaag agagcatgcc gtttattgca ttcccggcgt ctctcacccg
     7261 gcgcgatcct cagagcgccg aagatcgtcg cgtctagccc ttcccctctc gccgtccaag
     7321 gaactccaac ctaaacaagt atcggtcgat cggctcaatc ggcttgagtg atatcatcac
     7381 aacaacccga aaaatcgtcg caaaactacc acttttctgt cttattaagc gaactggcta
     7441 ggacttcggg ctccgcgcga caatggccat cgctcgacca aggccgcgaa aaagcccata
     7501 tcgcggcctg gcggatggtg ttctctgagc aaggcgcggg ccaggcgccg aacgccgcgc
     7561 gaggcggccg aaccgggata agccgtcaga aatggcttgc ggccgcgcac tgccagtggg
     7621 acgtgaggat cggcgcgtat tgaacccgtc ccgagtggtg aaatgacagc gcccaagaac
     7681 tgtcgggtcg aggcgatgaa ctggtccagc acttcgacgg attcggatgt ggaagtcgtt
     7741 tgattgagga gcacccgcag ccgaggctgg acggccagct tgcgaaacct gcagatggcg
     7801 gcgtgcgcgt ccgcaatcga cgtcggctcg ggtgtactga cgataacagc ctgctgagcc
     7861 gcggaggcca gcaagccaac acccgggccc agacccgagc ccgcgtccac caacacaaag
     7921 tccgactctg cctcgagctc ggtcagttcc gcgaccagcc gagccgcccc tcctgcgagg
     7981 tcttcctccc cggttcgtat ggcgtgcgcc cccggcacga tctgaatatc gcccgggccg
     8041 ctcacaagag cgtcgaccaa cctgcaacgg cccgctagca cgtcccccaa atcgtggcgc
     8101 ggcgtcaaac cgcacaagag atcgaggttt gccagtccga tgtcggcatc cagcac
//
LOCUS       DATAQL010001200         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2107392, whole genome shotgun sequence.
ACCESSION   DATAQL010001200 DATAQL010000000
VERSION     DATAQL010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107392"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2538)
                     /gene="glnE"
                     /locus_tag="VHS97_20700"
     CDS             complement(<1..>2538)
                     /gene="glnE"
                     /locus_tag="VHS97_20700"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX3450687.1"
                     /translation="LSATLRLLAENHRTTELLLQVFSTSQYLSEVLIRDSELLDWLQG
                     GPERRDRSALIDELWATVAGLPEEDDQKLALRRFRRRESLRIGYNDIVRGFPLEVITQ
                     DLSDLADACVEAAVRLARGRAEARFGAAMTARGSAARFVVLGLGKLGGQELNYSSDID
                     LVFLYEEDGQTQGPKSVSNSEFFARMGSETLRLLADHTALGIAYRVDMRLRPDGEQGV
                     LAQSLDATLGYYVTRGRTWERQALIKCRAIAGDLALGETFLEAITPFVYRRYLGAAEI
                     SEIKALKRRIEQRTVTAGTAEVEVKTGRGGIRDVEFVVQFLQLLHGGEYPEVRHATTL
                     LAIARLEQVGCLSAEERHIMNDTYRFLRQVEHRLQILFDRQTHEMPRDLEAVRILALR
                     MGYAPASAWEERNGPATRFMTDYRSKTELNRRILNHLLHDAFSGDAGQAVDPIVDLVL
                     DPQPSAEFIKVVLAPYPFRDHAVAYHNLMALAREDFAFLSHARCRHFLAAIAPRLLES
                     VSRTPDPDMTLTNLEKVSASLGAKAILWELFNFNPPSLRLYVELCATSQFLSEILINN
                     PGMIDDLVDSLVVDRPLPGAAIKAELAELCKGAEDLAPILWSFRNKEWVRIGTRDILG
                     REPIREVTRELADVAEAIVTQVARDQWKRKVERCGTPCCPATGRRDRWAILALGKFGG
                     RELNYHSDLDLVFVHEADGFTVGGRKSISNEQFVTEVAQKILKALGSGSATGPLYAVD
                     ARLRPHGASGPLVQTLGSFLAYFQRSTQVWERMTLTRARVIFATGGFGQALTDAVRSV
                     LAGPVDRELVASEVIAMRRKLEASRPRLDLKRGPGGLADLEFVVQYLL"
BASE COUNT          472 a          819 c          795 g          452 t
ORIGIN      
        1 caacaggtat tgcacgacaa actcgaggtc ggcaagcccc cccggccctc gcttcaagtc
       61 aaggcgcggg cgcgaggctt ccagcttcct gcgcatggcg atcacttccg acgccaccag
      121 ctcgcgatcc actgggccgg ccagcaccga tcgaacggcg tcggtaagcg cctggccaaa
      181 gccgccggtg gcgaagatca cccgagcgcg cgtcagcgtc attcgctccc agacctgggt
      241 tgaccgctgg aaataggcaa ggaacgagcc caaggtctgc acaagcggac cggaagcccc
      301 gtgcggacgc agccgggcgt cgaccgcgta cagcgggcct gttgccgagc cactcccgag
      361 cgctttcaat atcttctggg caacctcggt gacgaattgc tcgttcgaga tcgacttgcg
      421 gccgccgacg gtgaagccgt cggcctcgtg cacgaagacc aggtcgagat cgctgtggta
      481 gttcagctca cgaccgccaa atttgccgag tgccaggatt gcccatcggt cgcgacgacc
      541 ggtcgctgga cagcacggtg tgccgcaccg ttccaccttg cgcttccact gatcgcgcgc
      601 cacctgggtg acgatcgcct ccgccacatc agctagctcg cgcgtgacct cgcgtattgg
      661 ctcgcggccc aagatgtcgc gcgtgccgat gcgtacccat tccttgttgc gaaagctcca
      721 gaggatcggc gccaggtctt cggcaccctt gcacagctcg gccagctcgg ccttgattgc
      781 cgcacccggc aaagggcgat ccaccaccag cgaatccacc aggtcgtcga tcattcccgg
      841 gttgttgatc aagatctccg agaggaactg gctggtcgcg cacagctcga cgtacagtcg
      901 caggctgggt ggattgaaat tgaaaagttc ccagaggatc gccttggccc cgagcgaggc
      961 ggagactttc tcgaggttgg tcagtgtcat atccgggtcg ggcgtgcggc tcaccgactc
     1021 caagagccgc ggcgcgatcg ccgccaagaa gtgccggcag cgggcatgcg ataaaaaggc
     1081 gaaatcctcg cgagccagag ccatgagatt gtggtaggcc accgcgtgat cccggaacgg
     1141 gtaaggtgcc agcaccacct tgataaactc ggcgctcggc tgcggatcga gcacaaggtc
     1201 gacgatggga tcgacggctt gtccagcgtc gccgctgaac gcgtcgtgga gcaagtgatt
     1261 gagaatacgc cgattaagct cggtcttgct ccgatagtcg gtcatgaacc gcgtggcagg
     1321 cccgtttcgt tcttcccacg cactcgccgg tgcgtaaccc attcgcaggg ccaagatccg
     1381 caccgcttcc agatcgcggg gcatttcatg agtctggcga tcaaaaagga tctgcagccg
     1441 gtgttcgacc tggcggagga accggtaagt gtcgttcatg atgtgccgtt cctcggcgct
     1501 caagcagccg acctgctcga gccgggcgat ggccagaagc gtggtcgcgt ggcgaacctc
     1561 gggatactcg ccgccgtgga gcagctgcaa gaattgcacg acaaactcga cgtcacgaat
     1621 cccccccctg cccgtcttga cctcgacctc ggccgtgccg gccgtgaccg tgcgctgctc
     1681 gatcctccgc ttgagtgcct tgatctcgga aatctcggcc gcgcccaagt agcggcggta
     1741 gacaaacggc gtgatggcct cgaggaaagt ctcgcccagc gcgagatcgc ctgctatggc
     1801 gcggcacttg atcaatgcct ggcgttccca ggtgcggcca cgggtcacgt agtagccgag
     1861 cgtggcatcg agtgattgcg ccaaaacgcc ttgttcaccg tccggacgca gccgcatgtc
     1921 gacgcgatag gcgatgccga gcgcggtgtg gtcggccagc aggcgcaggg tttcgctccc
     1981 cattcgggca aaaaactcgc tgttggacac gctcttgggg ccctgggtct ggccgtcttc
     2041 ttcatagagg aacaccagat cgatgtcgga gctgtagttg agctcttggc cgccgagttt
     2101 ccccaagccc aggacgacga atcgtgcagc ggacccccgc gcggtcatgg ccgctccgaa
     2161 ccgagcctcg gcccggccgc gcgccagccg cacggctgcc tcaacgcatg cgtcggccaa
     2221 gtcggacagg tcctgggtga tcacttccag gggaaaaccg cggacgatgt cgttgtagcc
     2281 gatacggagg ctctcccgcc ggcggaaccg gcgcaaagcg agcttttgat cgtcttcctc
     2341 gggcagcccg gccacggtcg cccatagctc gtcgatcagg gccgaccgat ccctgcgctc
     2401 gggccctcct tgcagccaat cgagtagctc tgagtcgcgg atcaagactt cgctcagata
     2461 ctggctggtg ctgaaaacct ggagcaaaag ttcggtcgtg cgatggttct cggcgagtaa
     2521 acgtagcgtc gcgctcaa
//
LOCUS       DATAQL010001201         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4854791, whole genome shotgun sequence.
ACCESSION   DATAQL010001201 DATAQL010000000
VERSION     DATAQL010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4854791"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1676)
                     /locus_tag="VHS97_20705"
     CDS             complement(12..1676)
                     /locus_tag="VHS97_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450688.1"
                     /translation="MPRITASVVLLILGLSTASLAQAPAGRAEPRSYSVESPLAMPFP
                     ARSPTLVFMEEVQRELKLTETQKQRHTALRGQQQARVQKAREDYEDSQIFQAARNAIY
                     DDWLSAIDDVLEPGQRERFEQIQLQAQGPLAFEKRAIRHTLELSDDQIDKIEVIVDEG
                     IKELRNTSSVVVALEPGDDPQTLEGIRELVGGLEFQLAKERAQHMVLAGRAALMLRIA
                     KVLTVPQRAAYQKMLGAPFEVTRLRSPQLSSNELDVRQVARSLGLLGQRADKEFDVKI
                     AHPAYTIPRPRVLIDEAHHNFHTSSGRYKPFAALMSNDGYQVSPNRDKITGESLRKCE
                     VLVIANAMAAESMGSPGADKSAFTDDECKAIEYWVKAGGALLLITDHEPFGSSAESLG
                     KRFGVEMSKTVARDPASLATDSRGLLFSRDKKLVGDHPITRGRNESERINRVKTFTGQ
                     ALKGPEGSTPLLNFASTASIRGGKKGVSADGQSQGAALKFGKGRVVVLGEAAQLSAQL
                     TGMERTPNNMMGMNAPGSDNRQFALNIMHWLSGLLEPEELARNARP"
     gene            complement(1835..>3956)
                     /locus_tag="VHS97_20710"
     CDS             complement(1835..>3956)
                     /locus_tag="VHS97_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3450689.1"
                     /translation="NITMLGLILAIGIVIDDAVVVHENIFRHMEEDGMDGMAAARRGT
                     REIALAVLATSLSLVVIFVPIAFMGGIVGRFFSSFGLTVAFAVIMSLFVSFTLTPMLC
                     SRFLKLEPTPAGAAHGPKSKSGIFYRLIDGAYGGVLRGAMRHKFLVVVLTAIVVYSTV
                     PIGRLMGISLIPRDDQSEYEISVTTPEGYSLERTSQITAELESRIWKLKGTEHVFTTI
                     GQTEGGRVVKGEGDVTRGTIYVRITDLEKRNAAVPVRGWWDVTGQVRRFFTGAEFDQF
                     EVQQEARKFLEDYPDLRVSVNDVSPFQGGRRPQTFQVSLAGPELSQLAAYGDQLIADL
                     RKEGGIVDLDTTLSLRKPEVQVVIDREAASDLGVPVGTIADTLRVLVGGLPVSKFREG
                     DEQYDVWLRAESGKRSTSQDLYQITFPSPAVGLVKLASLAKLNEDRGPTEIERLSRER
                     IVTVVGNPEGIALGEAITKAERILKKMNLPPQYSYVFTGQAKTLGETGYYFMVAFTLS
                     ITFMYLILAAQFESWMQPIAILMALPVTIPFGMLSLVLFHTPMDLYAMFGLFMLVGIV
                     KKNGILQVDATNQLRAKGESRHDAIMAANHTRLRPILMTTVMLVAAMVPIALGNGPGA
                     GARASMAKVIIGGQMLSLVLALLVTPVFYALLDMFVNFTRRLGIRFSVETPPRPGHEP
                     HLHPQHAANGVGVEIYEEPAKSYA"
BASE COUNT          759 a         1219 c         1165 g          813 t
ORIGIN      
        1 aaacagacgc ctcaaggtct ggcgttccgc gcaagctctt ctggctcgag caaacccgag
       61 agccagtgca tgatattgag tgcgaactgg cgattgtcgc taccaggcgc gttcatcccc
      121 atcatgttgt tcggggttcg ctccatcccg gtcaactgtg ccgatagctg tgcggcttcg
      181 ccgagaacca caacccgccc cttgccaaac ttgagagccg cgccctgcga ttgcccatcc
      241 gcggaaacgc cctttttgcc acctcgaatg ctcgccgtac tggcgaaatt gagtagagga
      301 gtactcccct cgggtccttt gagtgcctgg ccggtgaagg tcttgacgcg attgatccgc
      361 tcggactcat tccgccctcg agtgatcggg tgatcgccca ccaacttttt gtcgcggcta
      421 aagagcaggc cgcgagagtc agtagccaaa ctagctgggt cgcgtgccac cgtcttgctc
      481 atctccactc cgaaccgttt ccccaggctt tcggcgctcg agccgaacgg ttcgtgatcc
      541 gtgatcaaga ggagcgctcc tcccgccttg acccaatatt cgatggcctt gcattcgtcg
      601 tcggtaaagg ctgacttgtc cgcgccgggg ctccccattg attcagccgc catggcattg
      661 gcaatcacca gaacctcgca ctttcgcaat gattccccgg tgatcttgtc tcggttgggc
      721 gagacctgat agccgtcgtt gctcatgagc gcagcaaacg gtttgtaccg gccgctcgat
      781 gtgtggaagt tgtggtgagc ctcgtcgatc aagaccctgg gccgcgggat cgtgtaagcc
      841 ggatgggcga ttttgacgtc gaactcttta tcggcccgtt gtcccaaaag ccccaaggaa
      901 cgcgcaacct gtcttacatc gagttcgttc gagctgagct ggggtgatcg gagcctcgtc
      961 acttcgaatg gcgcgccgag catcttctgg taggccgcgc gctggggtac agttagcacc
     1021 ttggcgattc ggagcatgag agcggctcgt ccggccagca ccatgtgctg ggcccgctcc
     1081 ttggcaagtt gaaactcaag accgccgacc agttcccgga tcccctcaag ggtttgtgga
     1141 tcgtcgcctg gctccaaagc gacaacgacg gacgacgtat ttctcagctc ctttatgccc
     1201 tcgtcgacga tcacttcgat cttgtcaatc tgatcgtcgg agagttcgag cgtgtgacgg
     1261 atcgcccgct tctcaaatgc cagcggacct tgcgcttgaa gctgaatctg ttcgaatcgc
     1321 tctcgctgcc cgggttccag cacgtcgtcg atagcggaca gccagtcgtc gtagatcgca
     1381 ttgcgcgcgg cctggaagat ctgcgagtct tcataatcct cacgcgcctt ttgcaccctc
     1441 gcctgttgtt gccccctgag cgcggtatgc cgctgcttct gtgtctcggt tagcttcaat
     1501 tcgcgctgca cttcctccat gaacacgagc gtagggcttc gcgcggggaa cggcatggcc
     1561 aagggacttt caaccgagta ggaacgcggt tcggcacgcc ccgcgggcgc ctgggccaac
     1621 gacgcagtgc tgaggcccaa aattagtaat acaaccgatg cagtaattct cggcatggca
     1681 tgacctcccg ggtgagggca ggacaatccg cgtgagttgc tccccttctg taaacagcgt
     1741 caaaatggga aaataagacc tcatctcgcc ggtccgcgcg cacaggaggc caaagctgcc
     1801 ggcaggttgg cttggacgac aggtgcgtgc ttcgtcaagc gtacgacttg gctggctctt
     1861 cgtatatctc gaccccgacg ccgttggccg cgtgctgggg atgaagatga ggctcatgcc
     1921 caggccgggg cggcgtttcg accgagaagc gaatgcccag gcggcgagtg aagttcacga
     1981 acatgtcgag caaggcataa aacaccggcg tgaccagcag ggccaacacg agcgaaagca
     2041 tctggccgcc gataatcacc ttggccatgc tggcgcgggc tccggcccct ggaccgttgc
     2101 ccagcgcgat cggcaccatg gccgccacca gcatgaccgt tgtcatcaag attggccgta
     2161 gccgcgtgtg gttggccgcc atgatcgcgt cgtgccgcga ttcgcctttg gcccggagct
     2221 ggttggtggc gtcgacctgc aagatgccgt ttttcttgac gattcccacg agcatgaaca
     2281 ggccgaacat cgcataaaga tccatgggcg tatgaaacag caccagcgac agcatcccga
     2341 acggaattgt gaccggcagc gccattaaga tggcgatcgg ttgcatccag ctctcgaact
     2401 gggcggccag aatcaggtac ataaacgtga tcgacaacgt gaaggcgacc atgaagtaat
     2461 agccggtctc acccagcgtc ttggcctggc cggtgaacac gtaggaatat tgcggaggca
     2521 ggttcatctt tttcaggatc cgctcggctt ttgtgatcgc ctcgcccaaa gcaatgcctt
     2581 cggggttgcc cacgacggtg acgattcgct cgcggctcaa gcgctcgatc tcggtcggcc
     2641 cgcggtcttc gttgagtttg gcaaggctag ccaacttcac caggcccacg gccggcgagg
     2701 gaaaggtgat ctggtagaga tcttgactgg tcgagcgttt gcccgactcg gcgcggagcc
     2761 agacatcgta ttgctcgtca ccctcgcgaa actttgagac gggcaagccg ccgaccagga
     2821 cccgcagtgt atcggcgatc gtgccgaccg gaacacccag gtcgctcgcc gcctcgcgat
     2881 cgattacgac ctgtacctcg ggtttgcgca aggagagcgt cgtatccagg tcgacgattc
     2941 ctccttcttt ccggagatcg gcaatgagct ggtcgccgta tgcggcaagc tggctcaatt
     3001 cggggcccgc gaggctcacc tggaacgtct ggggccggcg cccaccctgg aacggcgaga
     3061 cgtcgttgac gcttacgcgc aggtcggggt agtcttcgag aaacttgcga gcctcttgct
     3121 ggacctcgaa ttgatcgaac tcggcgccgg tgaaaaaccg gcgcacctga ccggtgacgt
     3181 cccaccagcc gcggaccggg actgccgcgt tgcgcttctc cagatcggtg atccggacat
     3241 agatcgtgcc ccgcgtgacg tctccctctc ctttgaccac gcggccgcct tccgtctggc
     3301 cgatggtggt aaagacgtgc tcggtgccct tgagcttcca gattcgcgac tccagctcgg
     3361 ccgtgatctg actcgtccgc tccagactgt agccctcagg tgtcgtgacg ctgatttcat
     3421 actcgctttg atcgtcacgc gggatcaacg agatgcccat gagtcgcccg atcggcaccg
     3481 tgctgtagac cacgatggcc gtcaagacta cgaccaaaaa cttgtgacgc atcgcccctc
     3541 tgagcacgcc gccataggcg ccgtcgatca acctgtagaa gattcctgac ttcgatttgg
     3601 gcccgtgtgc cgcgcccgcc ggggtcggct cgagcttcaa aaagcggctg cagagcatcg
     3661 gtgtcagcgt gaaagagacg aaaaggctca tgatcaccgc gaaggcgacc gtcaatccaa
     3721 agctggagaa gaatcggccg acgatccctc ccatgaatgc gatagggaca aagatgacca
     3781 cgagcgagag gctggtggcc agcaccgcca gggcaatctc acgcgtcccc ctgcgtgccg
     3841 ccgccatgcc gtccatcccg tcttcttcca tgtgccggaa gatgttttcg tggacgacta
     3901 ccgcatcgtc aatcacgatg ccgatcgcca ggatgaggcc gagcatcgtg atattg
//
LOCUS       DATAQL010001202         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1496906, whole genome shotgun sequence.
ACCESSION   DATAQL010001202 DATAQL010000000
VERSION     DATAQL010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496906"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VHS97_20715"
     CDS             <1..1365
                     /locus_tag="VHS97_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450690.1"
                     /translation="WHFYIDDHEEARRHIEDVVAKTQPGSGFNYVPGLGQSSLPLINS
                     EYGAVSAGSGDRDISWGLRDLTTQLRRQRKIQGFVYTELADIEWEHNGLVNYDRTPKR
                     FGYETWVPDMRPNELLGADFIGYDAPPAIVGKPGETVTIPIFVSHFSDRRGPVKLRWW
                     VGGYDSRADSRTVNLPSDVPIQWRPYDVVDLAPIKVTLPDYPFVGALALTLRDDQNQR
                     FAANFVNLVVKPAHPLPRIQRRGPKDVAIRFAPTDFARLEWSDAAKPPAGKVYGHGKG
                     FFEYRIEVPSAVVKAHPESVYYLFQAASKARGERADWPARVNRQDYPQSDMMRTWRSS
                     LVVSLNGRAVDRIELPDDAADARGVLSHLAKVEHGSHGELVDGKAALTDRDRVLLAAG
                     EPLVLRLAVPQDSPQAGGGLCIFGASTGELPLDPTLEIHTRDPLPADLGVDPQAPSAV
                     PSRP"
     gene            complement(1367..1642)
                     /locus_tag="VHS97_20720"
     CDS             complement(1367..1642)
                     /locus_tag="VHS97_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450691.1"
                     /translation="MEEPLPDENWQDLKIDLAARVRAVRIALYGENGGPLLAQALGIP
                     FRTLHNYESGYTIPAHSILRFIKLTRVHPHWLLTGNGEQFLDPDQSS"
     gene            2160..2780
                     /locus_tag="VHS97_20725"
     CDS             2160..2780
                     /locus_tag="VHS97_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450692.1"
                     /translation="MVDLLAVRVEQAYGLRVRRWWRGCSTSRVWHAAALRLWEAHAHD
                     PECVPLDVELFVASQPISVSLPDPWTELTHHDAAKRFRISVKRIIRRLRTELRREVLR
                     GERSIEQGEKVETMLGRTTRRLSAMGSYILAIRAGRDDLAERFAAAAAAQHQSCPLYR
                     LASMSLLPVDLYPDESLAVNAARNRRAEPAASATFSLHRNYKECLN"
BASE COUNT          580 a          842 c          836 g          602 t
ORIGIN      
        1 tggcatttct acatcgatga ccatgaagaa gctcgccggc atatcgagga tgtcgtcgcc
       61 aagacccagc ctggcagcgg cttcaactat gtcccgggac tagggcaatc gagccttccc
      121 ttgatcaact cagaatatgg agcggtctcc gctggaagcg gcgaccgcga tatctcctgg
      181 ggtctgcgcg acttgacgac ccagttgcgt cgccagcgca agattcaagg tttcgtctac
      241 accgagctcg ccgacattga atgggagcat aacggcctgg tcaactacga ccgaactccc
      301 aagcgattcg gctacgagac ctgggtaccc gatatgcggc ccaatgaact cttgggcgcc
      361 gactttatcg gctacgacgc acctccggcg atcgtgggca aaccgggaga gactgtgaca
      421 attccaatct ttgtcagcca tttttccgac cgccgaggac ccgtcaagtt gcgctggtgg
      481 gttggcggtt acgactcgcg cgccgatagt cgaaccgtga acttgccgag cgatgtcccg
      541 atccagtggc gtccgtacga tgtggtcgac ctggcgccaa tcaaagtcac gctgcccgat
      601 tatccgtttg tcggagccct cgcgctcacg ctgcgcgacg accaaaacca gcggttcgcg
      661 gcgaatttcg tcaacttggt ggtcaagccc gcgcatccgt tgccgcggat ccagcgccgc
      721 ggtcctaagg atgtcgccat ccggtttgct cctacagatt ttgcgcgtct cgaatggtcc
      781 gatgcggcca agccacccgc cggcaaggtc tacggtcacg ggaaagggtt tttcgagtac
      841 cggatcgagg ttccgtccgc tgttgtaaag gcgcatcccg aatcagtcta ttacttgttt
      901 caggcggcat ccaaagcgcg cggggaaagg gccgattggc ccgcacgggt gaaccggcaa
      961 gactatccac agtcggacat gatgcgcacc tggcgctcga gcctcgtcgt ctctttgaac
     1021 ggtcgcgcgg tcgaccggat cgagctgccc gacgacgctg ccgacgcacg tggagttctc
     1081 tcgcacctgg ctaaggtcga gcacggcagc catggggagt tggttgatgg caaggcggcg
     1141 ctcaccgatc gcgatcgtgt ccttctggcc gctggcgagc cgcttgttct tcgtttggcc
     1201 gtcccgcagg attcgcccca ggccggcggc ggcctgtgca ttttcggggc ctcaaccggt
     1261 gagctgccgc tcgatccaac gctcgagatc cacacacgag acccgcttcc cgctgactta
     1321 ggcgtcgacc cgcaggcacc gtccgctgtt ccttcacgcc cctgaatcac gaggactgat
     1381 caggatcgag aaactgttca ccgttgccag tcaagagcca gtgggggtga acgcgggtca
     1441 gcttgatgaa gcgtagaatc gaatgagccg ggattgtgta tccggactcg tagttgtgca
     1501 acgtccggaa cggaattccg agcgcctgag ccaatagtgg cccgccgttt tcgccgtata
     1561 gtgcgatccg aactgcgcga accctcgctg ccaggtcgat cttgaggtct tgccaattct
     1621 cgtcaggaag tggttcttcc atcgaattgc cgccgttgca atcaaaaacg attttcggat
     1681 cggcaggacg tcgtactgaa gacccaatcc ttaaattatg cgcccggtca caaagaaatg
     1741 ccagtgccac gcatcgttgc acggttcgta caattcaacc gttggtgacg tcagcgaacg
     1801 ctgcgagcat tgcggcctga aaatgcgtga tcggatgcta ccaattacaa gcggcgatac
     1861 atggaaacgt gcggcacgca cttgttgagt caatgcacat cgacgaccag tcggtcgggt
     1921 atctaagtaa aatcgccgtc gacacctatg acgtttcttt tcgttcagga cgagcctgcc
     1981 gagctcggcg gcgttgaatg aggggaggac tttcaaaaaa aatttctaat ctgatgacgt
     2041 catcgcgcgg caatttcggt tcctacctca gcagtaaatg cgacggcctg tttgcggaga
     2101 gcggagccgg gtatagacgc ttacaacaga gggtgagcca cgtgttgccg actcatcaaa
     2161 tggtagatct cctagccgtt cgtgtcgagc aggcctatgg tttacgtgtg cggcgctggt
     2221 ggcgtggttg ttccacatcg cgcgtctggc acgctgcggc gctgcgactc tgggaggccc
     2281 atgctcacga tcccgagtgc gttcctcttg acgtcgaatt gttcgttgca tcgcagccaa
     2341 tctctgtctc gctacccgac ccatggacag aactgaccca tcacgatgcg gcaaagcgtt
     2401 tccggatctc cgttaagcga attatccgac ggctgcggac cgagttgcga cgagaagtgc
     2461 tgcgtggtga gcggtcaatt gagcagggag aaaaggtcga aacgatgctc ggtcgcacga
     2521 ctcgacgcct gtcggccatg ggctcgtaca tcttggcgat acgcgccggc cgggacgacc
     2581 ttgcggaacg ctttgccgcg gccgccgcgg cccaacatca atcgtgcccg ctttaccgtt
     2641 tagcgagtat gtcactgctc ccagtcgatc tctatcctga cgagagcctc gccgtcaatg
     2701 cggcgagaaa ccgcagagcg gagccggcgg catctgcgac gttttcgttg catcgaaact
     2761 acaaagaatg tctcaattag gttgaagtcg aacaccgacg gctgaaagac tgaacgggca
     2821 ttcgcaccta cctaactcga atccatgcct cgtagccggt
//
LOCUS       DATAQL010001203         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_274853, whole genome shotgun sequence.
ACCESSION   DATAQL010001203 DATAQL010000000
VERSION     DATAQL010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274853"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1651
                     /locus_tag="VHS97_20730"
     CDS             446..1651
                     /locus_tag="VHS97_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEX3450693.1"
                     /translation="MVENRSRRRFALPHVALIVETSTAYGRAILSGISQYVREYGPWT
                     VYIEQRSLQDPAPPWLERWAGDGIIARASTPQSARKLAKTGVPTVDLNDQVRGLGLPQ
                     IHSDHAAIARLAADHLIDRGFRHFAYFGFPIFEWSVRRQEAFTNHVLAAGHQFHEGLP
                     GRRVHWSHQQASWEDEIEGVAQWVKRLPKPLGIMAGNDIRGIQMLDACRRAGVAVPEE
                     VAVVGVDNEELVCLLAYPPLSSVVPDAYRVGYESAALLDQMMKGHPVTEMLRSIPPVS
                     VATRQSSDVTAIANPCLASAMQFIRESACQGIGVDDVLAHLSVSRSVLQRLFRKELGQ
                     TILDAITATRMQRVKQLLAETDLSLNDIADRAGFSYVEYLSTSFRHHTGQSPSAYRRK
                     FRRRPGQPP"
     gene            1830..3026
                     /locus_tag="VHS97_20735"
     CDS             1830..3026
                     /locus_tag="VHS97_20735"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX3450694.1"
                     /translation="MLRPLVVLILLAAQADAQEYPRWTQLSSKSGDLPAPGKSQQQTG
                     CLVLDIDKNGLNDIVITSRNAGSRMVWYQRSKAGWTIYPIDRGLDIEAGGASADIDGD
                     GDLDLAFGEDHSGSKMFWWENPYPLYAADRPWVRREIKSTGGTMHHDQIFGEFGDDGR
                     LQLAFWVQKAGTLFLANIPRDLKKTEPWVTLPIAKVGPAEGLAKADIDCDGKCDLIGG
                     GYWFKYLERSSQYQPMLIEQGLLSTRVRAGQIVEGGPPEVVFVAGDAIGPLKWFEWRR
                     GDWVGHDLLIENVVHGHSLELADIDQDGHLDIFCAEMAQWTESAKLPDNPSARTWIFY
                     GDGRGRFTKTLLATGLDNHESRVADLDGDGDLDIVVKPYTRDTPRLDVWLNQGNGPKK
                     SIATPD"
     gene            3157..>3422
                     /locus_tag="VHS97_20740"
     CDS             3157..>3422
                     /locus_tag="VHS97_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450695.1"
                     /translation="MEFNRFDVATKELVWEDPSAWLQRLGIACAGVVELIDSDITTLT
                     ASADKVIRVSGPEPYLVNIELHSYHDSGLARTLWYRQVALDYRH"
BASE COUNT          665 a         1055 c         1022 g          680 t
ORIGIN      
        1 gtgacttgcc ctcttcgcaa acagaatcaa actcggcggg cgcgccctct tggggaatca
       61 cggttgcacg accaacccgc gcctcgcctg gtcgcgaatt atgcacgacc tacgacaaag
      121 gcctaccccc cgacctgagc gaatttctca tcctcggcag cacgcggcgg acgttgcccc
      181 gaccgtcagg cttgagcata cgcgctgtaa tgttcgggtc catgaaattt tgggtttttt
      241 tcctataact ttcggtcacg gcgatcgccg gttagaatta cgctttgctc gcgtcatagc
      301 tactgtcgta acgaggctcg cggccgtgaa atcgacccgg ttgacgacgc cagcgggcgc
      361 ctgcctgcac aatccggtca gagtatctgc tccctgaccc gactcgcggg aggccattcg
      421 cgatactgat tgcggaccca gttccatggt cgaaaatcga agccgccgcc ggtttgctct
      481 cccgcatgtc gccctgatcg tggaaacttc caccgcctac ggccgggcca tcctgagcgg
      541 catttcgcag tatgtccgcg aatacgggcc atggaccgtc tacatcgagc agcgctcctt
      601 gcaagacccg gctcccccat ggctcgagcg ctgggctggt gacgggatca tcgcccgcgc
      661 atcaacccct cagagcgctc gcaagctggc gaagacgggc gtgccgacgg ttgacctgaa
      721 cgatcaggtt cgcgggctcg gcctgccgca aatccacagc gaccatgcgg ccatcgcccg
      781 actggccgcg gaccatctga tcgatcgtgg ctttcgtcat ttcgcctact tcggctttcc
      841 gatattcgaa tggtcggtgc ggcgtcagga ggccttcacc aaccatgttc tcgcggccgg
      901 tcatcagttc cacgagggtc tgcccggtcg ccgcgtccac tggagtcatc agcaagcttc
      961 gtgggaggac gaaatcgagg gggttgcaca atgggtgaag aggttgccca agcccctggg
     1021 gatcatggcc ggcaatgata tccgcgggat ccagatgctc gacgcgtgcc ggcgggccgg
     1081 agtggccgta cccgaagaag tcgcggtcgt cggcgttgat aacgaagagc tcgtctgcct
     1141 gctcgcctat ccgccccttt cgagcgtagt gcccgacgcc taccgagtcg gctacgaatc
     1201 ggcggctttg ctcgatcaga tgatgaaagg acaccccgtc accgaaatgt tgcgatcgat
     1261 cccccccgta agcgtcgcca ccaggcagtc atccgacgtg acggcgatcg ccaatccgtg
     1321 ccttgccagc gccatgcaat ttatccgcga atccgcctgc caggggatcg gggtcgacga
     1381 cgtgctcgcg cacctgagcg tctcacggag cgtgctgcag cgattgttcc gcaaggagct
     1441 gggacagacg atcttagacg cgattaccgc gacacgcatg cagcgcgtca agcagttgct
     1501 cgccgagacc gacctgtctc tgaatgacat cgccgaccgt gccggttttt cgtacgtgga
     1561 gtatttgagc accagcttcc gccaccacac cggccagtcc cccagcgctt atcgtcgcaa
     1621 gttcaggcgc cggccgggtc agccgccatg agtcgcccga gttgtgatcc gctcgaccaa
     1681 tctccggtcg catgcccatg gcagcggtgt cgccggctgc cgcgcaattg aaaaaggcag
     1741 gtcctaccgg gtagatttga cgagttgaat tatgctgcgc gcgcacccac gactgacggc
     1801 cgatggtcga gaggttgacg atgtatgtca tgctacgtcc cctggtcgtc ttgattttgc
     1861 tcgcggcgca ggccgacgcg caggaatacc cgcgctggac acaactgtcc agtaagtcgg
     1921 gagatctacc tgccccgggt aaatctcaac agcagaccgg ctgcttggtg ctcgacattg
     1981 ataagaacgg cttgaatgat atcgtcatta cgagccgtaa tgccgggtcc cgcatggtct
     2041 ggtatcaacg cagcaaggcg ggctggacga tttaccccat cgatcgcggc ctggacatcg
     2101 aggcaggtgg tgcgtccgcc gacatcgatg gcgatggcga cctggatctc gcattcgggg
     2161 aagaccattc cggctcgaaa atgttctggt gggaaaatcc ctacccgctt tacgctgccg
     2221 acaggccctg ggtgagacgg gagatcaaga gcactggcgg tacgatgcat cacgaccaga
     2281 tctttggcga attcggtgat gacggccgcc ttcaactggc tttctgggtg cagaaggccg
     2341 ggaccctctt tctcgccaat atcccgcgcg acctcaagaa aaccgagccc tgggtgacgt
     2401 tgccgatcgc caaggtcggg cctgccgagg ggctggctaa ggccgacatc gactgcgacg
     2461 gcaagtgcga cctgatcggt ggtggttact ggttcaaata tctcgaacgg agttcgcaat
     2521 accaaccgat gttgatcgag cagggcttgc tttcgacgcg ggtgcgggcc ggccagatcg
     2581 tcgagggagg accacccgaa gtggtcttcg tcgcggggga cgccattgga cctctcaaat
     2641 ggttcgaatg gcgccgcggc gactgggtcg gtcacgacct tctgatcgaa aacgtggtac
     2701 atggacatag cctcgagctt gccgatatcg accaggatgg gcatctcgat attttctgcg
     2761 ccgaaatggc tcaatggacc gagtccgcca agctaccgga caaccccagc gctcggactt
     2821 ggatcttcta tggcgacggc cggggacgat tcaccaagac tttgctcgcc accggcctcg
     2881 acaaccacga gtcccgcgtg gccgacctcg atggcgatgg cgacctcgat atcgtcgtca
     2941 agccttacac gcgcgatacg ccccggctcg acgtctggct caatcagggc aatgggccaa
     3001 agaagagcat cgccacacct gattgatcac caaacccaaa cccggcagga ttttcaaacg
     3061 gccgtggaga cggctcggcg gcagccactg ccttcatctg acgaactgac gagggatgtc
     3121 atagcgtgga ctggatgtcc ccgatggaga atgctggtgg aatttaaccg tttcgacgtg
     3181 gcgaccaagg agttggtctg ggaagatcct tcggcttggc tccagcgatt gggaattgcc
     3241 tgcgcgggag tggtcgagct gatcgactcc gacatcacga cattgaccgc ttcggccgac
     3301 aaggtcatcc gcgtgtcggg acccgagccc tatctggtca acattgagct tcattcctac
     3361 cacgattcag gcctggctcg gacgctttgg tatcgccagg ttgctcttga ctatcgccac
     3421 ga
//
LOCUS       DATAQL010001204         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1497051, whole genome shotgun sequence.
ACCESSION   DATAQL010001204 DATAQL010000000
VERSION     DATAQL010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497051"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /locus_tag="VHS97_20745"
     CDS             <1..1724
                     /locus_tag="VHS97_20745"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3450696.1"
                     /translation="IAVSPIRDRHGRLIGVSKIARDITERKRAEDALRSSERRYSLLF
                     ETMAQGVVYQDAEGGIVDMNPAAEAILKTTSVELKGQTSVDREHFTIRADGSPFRGME
                     HPAMVALKTGQIVRNVLMGVRDPKGGEYRWIDITAVPLFRDGENRPHNVCALFDDITE
                     RKQAEDAQRRYATRLGSLHDIDRAILAIQPPEKIAGVALRHLAKLVPCWRASVARIVQ
                     GALESRVLTSIGALSRRTAHGAKGTLTVLEPEDLETLKCGQSKIVDDLHNLTSMSKPL
                     KLMKDAGARSVARLPLLVRGRLIGSINFYSDRPGAYTAENVEVIREAANQVAIALHNS
                     ELYEENRVARERLEVLSRRLIRAQEDERRRIARELHDEIGQALTAVTVNLQSVDKRVA
                     VQDDRVAECIAIVDDTLGRVRGMALDLRPSLLDDLGLVAALQWYVERHARRTGMAGRF
                     IADPEKLRAAPEIETACFRVVQEALTNTARHARATRFSVELLQHNGGLELIVRDDGCG
                     FDPSESLERAAGGASLGLVGMIERIELIGGKIAFISAPGKGTEVQASIPRITPGSAAV
                     HQSGSSS"
     gene            1721..2374
                     /locus_tag="VHS97_20750"
     CDS             1721..2374
                     /locus_tag="VHS97_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3450697.1"
                     /translation="MKPIRVLLADDHTILRAGLRALLGNLPDLEIVGEAASGEEALIL
                     TEALRPDVLLCDISMPGQSGLEVAEQVARKFPATRTIILSMHSEKQYAVRALQAGAVG
                     YLLKDAGTTELELALRATASGGLYLSPAISKHIVADYTQMPSVRTEDADTLTPRQREV
                     LKLIAEGLTTKAIALRLDISAKTADTHRTQLMDRLGIHDIAGLVRYAIKVGIVSADE"
     gene            2798..3313
                     /locus_tag="VHS97_20755"
     CDS             2798..3313
                     /locus_tag="VHS97_20755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450698.1"
                     /translation="MVDEDTCPRQIDEPKGSRYGLFSAHSERELAPVDPEADTLIPIR
                     LMIVDNSLVFLGTLRRLLETTPGIEVVGSATSGRDALELVDRVHPDAVLTDLAMPELD
                     GLQMARLLAVRSGKPIVVIMSIHNLTSYRQAARAAGADAFITKSDLVNRIQPLLRHLQ
                     GQRESKGPEPS"
BASE COUNT          658 a         1021 c         1089 g          585 t
ORIGIN      
        1 cgatagcggt ctcaccgatc cgcgaccggc acggccggct gatcggggtc tcgaagatcg
       61 ctcgcgacat caccgaacgg aagcgggccg aggacgcgct gcggtcgagc gagcggcgat
      121 attccctctt gtttgagaca atggcacaag gcgtggttta ccaagacgcc gaggggggca
      181 ttgtcgacat gaaccctgca gcggaagcca tactaaaaac cacgtctgtc gagttgaaag
      241 gtcagacgtc ggtggaccgc gagcacttca cgatccgcgc cgacggttcg ccgttccggg
      301 gtatggagca ccccgcgatg gtggcgctga aaacgggaca gatcgtgcgc aacgtcttga
      361 tgggcgtgcg cgacccgaag ggaggtgaat atcgttggat cgacatcacg gcggtcccgc
      421 tgtttcgcga tggagaaaat cggcctcaca atgtatgtgc actattcgat gacatcactg
      481 agcggaagca ggccgaggac gcgcagcgcc gctatgcgac gcggcttggg agcctgcacg
      541 acatcgatcg ggcgatcctg gcgatccagc cgcctgagaa aatcgcgggg gtcgccttac
      601 gccacctcgc caagctcgtc ccttgctggc gcgccagcgt cgcgcggatt gttcaggggg
      661 cattggagag ccgggtcctc acgtcaatcg gcgccttgag tcgacggacg gctcacgggg
      721 caaagggtac actgactgtc ctcgaaccgg aagacctcga gacgctcaag tgtggccagt
      781 cgaaaatcgt ggacgacctt cataacctga cctcgatgtc caagccgctc aagttgatga
      841 aggacgcggg tgcgaggagc gtggcgcgcc tgccgctctt ggtccggggc cggctcatcg
      901 gctcgatcaa cttctactcg gatcgcccgg gggcctatac ggcggaaaac gtcgaggtaa
      961 ttcgcgaggc cgcgaatcag gtggcgattg cgctgcataa ttcagagctt tacgaggaga
     1021 atcgggtggc acgggagcga ttagaggtgc tgtcgcggcg gctcatccgc gcgcaggaag
     1081 acgagcggcg acggatcgct cgcgagcttc acgacgagat cgggcaggcc ctgaccgcgg
     1141 tgacggtaaa cctgcagagc gtcgataaga gggtagccgt tcaggatgac cgggtagccg
     1201 agtgtattgc gatcgtcgac gacactttag ggcgtgtacg cggcatggcg ctcgacttac
     1261 gcccctcact tctggacgac ctcgggctcg tggccgcact gcagtggtac gtcgagcgtc
     1321 acgctcggcg gacgggtatg gcgggccgct tcatcgccga ccccgaaaag ctgcgcgcgg
     1381 cccccgaaat cgagacggcc tgcttccgag tggtccagga ggcactgacg aataccgccc
     1441 ggcatgcgcg ggcgacccgc ttcagcgtcg agcttctaca gcacaacggc ggcctggaac
     1501 tgatcgtacg cgacgatggg tgcgggtttg acccttccga gtcgctcgag cgggcggcag
     1561 ggggcgccag cctgggactg gtggggatga tcgagaggat cgagctgatc ggtggcaaga
     1621 tcgcgttcat ctcggcgcct gggaagggga cagaagtcca ggcctcgatt cccaggatca
     1681 cgccgggctc cgccgcggtg catcaatcgg ggagcagttc gtgaaaccga tccgcgtcct
     1741 gctcgccgat gaccacacca tcctacgggc cggtctgcga gcactcttgg gcaacctacc
     1801 ggacctggag atcgtcgggg aggcagcctc cggcgaggaa gccctcatcc tcaccgaggc
     1861 tctccgtccc gatgttctcc tgtgtgacat cagcatgccc ggccaaagcg gcctggaggt
     1921 ggccgaacaa gtcgcacgga agttccctgc gacgcgtacg atcatcctgt cgatgcacag
     1981 cgagaagcaa tacgcagtaa gggcgctcca ggccggcgcg gtaggttacc tgctcaagga
     2041 cgcgggcacc accgaactgg agctcgccct gcgagcgact gccagcggcg ggttgtactt
     2101 gagcccggcg atctccaagc acatcgttgc tgattacact cagatgccct cggtccgcac
     2161 cgaggacgcc gatacgctta ccccgcgcca gcgcgaggtg ctgaagttga tcgccgaggg
     2221 gctgacgacc aaggcgatcg cgctccgact ggacatcagc gccaagaccg ccgacacgca
     2281 ccgcactcag ttgatggatc ggctcgggat ccacgacatc gccggcctgg tccgctatgc
     2341 catcaaggtc ggcatcgtct cagccgacga gtgaggactg cggccagcca atttcgattc
     2401 tgccaaaagg ctttctggcg cgatcgaacg ccgccagcct gtcaagagag ttccggagcg
     2461 gcgtctcagg aggattcctg agagacagac gcacggattc gatccatact ttgttgtttg
     2521 acgcatcggc gtccgagcgc cgcagttgat gccgactggg gccttgcagg tgatacatta
     2581 ccgtggcgag taggggctcg ggatctgttt gagatcccgc cctccgcgtc cctcctggta
     2641 cgacgtagcc aagtgggtgg tgacggtgcg agctcgcttc agaaagtcgc gtcttgaatc
     2701 cagtccagag gccgggtcat ctgtcgatgg cgaaccggtc actttcctgg ctggatcgga
     2761 tggctccgaa ccgggtcaac acagcgagcg gacacgaatg gttgacgagg acacttgccc
     2821 acgccagatc gacgagccaa aaggctcgcg gtacggcctt ttctcagccc attccgaacg
     2881 cgagctcgcg ccggtggacc ccgaagcgga cactctgatt cccattcgcc tgatgatcgt
     2941 cgacaacagc ctagtctttc tcgggactct gcggcgcctc ttggaaacaa cgccaggcat
     3001 cgaagttgtt ggatcagcaa cctcgggacg agatgccctt gagcttgtcg atcgagtgca
     3061 tcccgacgca gtgctgacgg acctcgccat gccagagttg gacggcttac agatggcacg
     3121 tctgctcgcc gtccggtcgg gcaagccgat agtcgtcatc atgtccatcc ataaccttac
     3181 gagctatcgg caggcggccc gggctgcggg cgctgacgcc ttcatcacca agtcggacct
     3241 ggtcaatcgg atccagccgc tgctgcgcca cctgcagggc caacgcgaat ctaaagggcc
     3301 agagccgtca tgagtccgac gacagagtca cgaccaaacg aatgcggact gga
//
LOCUS       DATAQL010001205         5594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4244611, whole genome shotgun sequence.
ACCESSION   DATAQL010001205 DATAQL010000000
VERSION     DATAQL010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4244611"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VHS97_20760"
     CDS             <1..575
                     /locus_tag="VHS97_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450699.1"
                     /translation="ASGRFVWRISSIGGTLLMVLAIGAGSIGLIFCASFVARQDQLAA
                     SGKDPLDAIVIRSEVIHLYLPRTLIELANQQTQQPPLQTHEELRRSLEVQHHPDLWYA
                     VTLRGFQANGEFADTTFIRRPYSEPDRFAEFLEIMAAATASPPKLWRNLTSATVHLIP
                     DELSERDPREVITELIGQSPPPTMSDSPPR"
     gene            828..1667
                     /locus_tag="VHS97_20765"
     CDS             828..1667
                     /locus_tag="VHS97_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450700.1"
                     /translation="MLRAWLKRLDVNVLSARPHMNSPSYRDCQRRRALLAVELLENRL
                     TPSHLTIFSATMPHGSAGSGVHANHGAATSAAKHITVAKVTHAADHSVTAKRASPAAS
                     VSKTTFTVSAPAAATAGKALNVIITAKNASGKTATSYSGNVALAASDGQVVHLVTARR
                     FVKGVAIETIVLYTADTLTLTAAAGAIKGTSAGISVKPAAASVFAISAPTRAMAGTGF
                     NVMLTAKDAYGNTVTGFSGNVNFASSDGQPLPVLGQTVWSNGAAIYAVVLNSPDTITL
                     TAA"
     gene            1704..2414
                     /locus_tag="VHS97_20770"
     CDS             1704..2414
                     /locus_tag="VHS97_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450701.1"
                     /translation="MPARTLSSFVVSASGAATAGTGFNVVLTALDNFGNTLTSFGGTV
                     TLTSSDGQPVHLLGAPVFSNGTALVTVTLDTADTLSLTAAAGVINGTSAGIRVQPAAA
                     SAFVVGAPSSAAAGTGFGVALTAKDAYGNTVSGFSGNVSFTSSDSQTAPVVARTAFSN
                     GTAIFAVVLNTPDTVQLTAASGTISGTSGSIIVGSASAAGDWFSQNLSDPGLRALART
                     DFNRDGSLTYSDMLGLFA"
     gene            2430..3578
                     /locus_tag="VHS97_20775"
     CDS             2430..3578
                     /locus_tag="VHS97_20775"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HEX3450702.1"
                     /translation="MITTAELQSLQALVTPSGAAAVNMASPVQSLTYKVVDGDPANAQ
                     YQGTALGNLQAGNPASHLQELVDKWFLGQDTPTIDMEYLSGSSVSYSLASGTLFGSGG
                     PSYKDVYQGEEGDCWLLSSLAVTAAHDPSLIQSMFTSDGTQMENGVPVQVWTVRFYDN
                     GVASYLTVNNLLPASSGNLVYAGGFQSISNPNNVLWVELAEKAYAQLCASGWNSRPES
                     NAFASLNGGSASTALPVIAGAQESTSNAFANSTSFINAIDAGMLLTLGTYPSGNNALG
                     IVGNHDYSVVGYNASNQTFTLLNPWGWNNNNAPGILNLTWTLLAENFFLDGNGNRPSS
                     ASLPQTGSDSSPSAGSMDTTGPMEAIIFSSSDDMGHGSAKAARSYWSD"
     gene            complement(3913..>5594)
                     /locus_tag="VHS97_20780"
     CDS             complement(3913..>5594)
                     /locus_tag="VHS97_20780"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX3450703.1"
                     /translation="MKRAKASLDDALPFAMEPVVYDFRINDHGTIATLDADSSAMMPP
                     PYYTIQVPRMVAEGTATLPYHRRADVDHFANRCPQTSELLRVFRTTINHLFPVTRSAD
                     DSSRAEWDAEAARIRRDNGFDPVQHEQLRDDLQHGRIGLARNRLPVDLDIRDVDDSEL
                     ILARDLGAGTAFGRGKSALERGEVAVVTLAAGVGSRWTTGAGVVKAVNPFVELDGRHR
                     SFLEIHLAKTKKAQREFKTQIPHIVTTSYLTHEAIERHLRSTSNYGHDGSVYLSRGRS
                     IGQRLIPMARELSYLWEEASHEKLDDNKEKVRDASRRAILDWAKGQGEGADYTDNVPI
                     QRFNPPGHFYEVPNLLRNGLLARLLDEHPNLQWLLVHNIDTLGASVAPAVLGMVVESG
                     ASLSFEVIPRRIDDRGGGLAKVAGRLRLLEGLAQPREDTEFKLRYYNTLTTWVRIDGL
                     LAAFGLSRGALKSDSQRIDAAVRAMAARIPTYVTIKEVKRRWGHGQEDVYPVTQFEKL
                     WGDMSSLPDLNCAFLAVDRQRGQQLKDTAQLDGWANDGSLAHVRSLCEFGR"
BASE COUNT         1079 a         1745 c         1661 g         1109 t
ORIGIN      
        1 gggcatccgg tcgattcgtg tggaggattt cgtcgatcgg cggcacgctc ctcatggtat
       61 tggctattgg cgctggatcg atcgggttaa ttttctgtgc cagctttgtg gctcggcagg
      121 atcagctggc tgcgtccggc aaagatcccc ttgacgccat cgtcattcgc tcggaggtga
      181 tccacctcta tctgccgcgg acgttgatcg aactggcgaa tcaacagacg cagcaaccgc
      241 ctttacaaac tcacgaagaa ttgcgacgaa gccttgaagt ccagcaccat ccggatttgt
      301 ggtacgcggt gactttgcga ggatttcaag ccaatggtga gtttgccgat accacgttca
      361 ttcggagacc ttactccgaa cccgaccgct ttgcagagtt cctggagatc atggcggccg
      421 ccaccgcgag cccgcccaaa ctatggagga acctcacgtc tgccacggtc catctcatcc
      481 ccgatgagtt gtcagagcga gacccacgcg aggtcatcac cgagctaatc ggccagtccc
      541 caccgcctac catgtcagat tccccccctc gatgagcttc atgccgaaaa aaccgaattt
      601 cccgcaaatt tgacacgcac gaaagtgtga cgtctatttc cgttaggttc ctgtccccgt
      661 aaggcatcgt tgcctgacgg aaccaccgga aagccaaagc tcgaattcgg cactcgtctg
      721 gcccttactc cagaacgcga gaacaacggc acatttttgc agtgccggct ctggctcaat
      781 catcgaatcg attgcttgtc ctctgagata accatcggag tacgctcatg ttgcgcgcct
      841 ggctcaagcg gttggatgtc aacgttttga gcgcacggcc ccacatgaat tcaccctctt
      901 atcgagattg ccagcgccgt cgcgcgttgc tcgccgtaga gcttctggaa aaccgtctca
      961 cgccgtctca tctgacgatt ttctcggcaa ccatgcccca cggctccgcc ggcagcggcg
     1021 ttcacgccaa ccacggcgcg gcgacgtcgg cggcgaagca tatcaccgtc gccaaggtca
     1081 ctcacgccgc cgaccacagc gtgaccgcca aacgggcttc ccccgccgct tccgtctcga
     1141 aaacgacgtt caccgtgagc gcgccggccg cggcgaccgc cgggaaggcc ttaaacgtca
     1201 ttatcacggc caaaaatgcc agcggtaaga cggccaccag ctacagcggc aatgtagcgc
     1261 tggccgcgag cgatggtcag gtagttcacc tcgtgacggc acgccggttc gtcaagggcg
     1321 tcgccatcga gacgatcgta ctctacacgg ccgatacgtt gacgctcacg gcggccgcgg
     1381 gtgcgatcaa aggcacgagc gccggcatta gcgtcaagcc cgcggcggcg tcagtttttg
     1441 ccatcagcgc ccccacccgc gcgatggccg ggactggctt caacgtcatg ctcacggcca
     1501 aagatgccta tggcaacacc gtcaccggct tcagcggcaa cgtgaatttc gccagcagcg
     1561 acgggcagcc tctgcccgtg ttaggccaga ccgtgtggag caatggtgcg gcgatatatg
     1621 cggtcgttct caactcgccg gatacgataa cgctcacggc ggcctaggga ccagtcgggg
     1681 tcacgagcgg cttcatcgac atcatgccgg cacgaacgct atcgtcgttt gtcgtcagtg
     1741 cttctggcgc ggctacggcc ggcaccggct ttaacgtcgt cctcaccgct ttagataact
     1801 tcggcaacac tttgaccagt tttggcggta ctgtgaccct taccagtagc gacggtcagc
     1861 ccgtgcatct cctcggggcg ccggtgttca gcaatggcac ggccctggtg acggttactc
     1921 tcgacacggc ggacacgtta tcgcttacgg ccgccgcggg tgtaattaat gggacgagcg
     1981 ccggcatccg cgtgcagccg gcggcggcgt ctgccttcgt cgtcggtgcc ccatccagtg
     2041 cggcggccgg gacgggcttc ggcgtggcac tcacggccaa ggatgcttat ggcaacacag
     2101 tcagcggctt tagcggcaac gtgagcttta cgagcagcga cagtcagacc gcgcccgtgg
     2161 tagcgcggac ggcgttcagc aacggcacgg cgatttttgc cgtagttctc aacacgccgg
     2221 atacggtgca gctcacggcg gcctcgggaa cgatcagtgg caccagcgga agcatcatcg
     2281 tcggctccgc ttccgctgcg ggtgattggt tcagccagaa cctgagcgat cccggactgc
     2341 gagcactggc acgaacggac ttcaaccgcg atggatctct cacctacagc gatatgttgg
     2401 gcttgtttgc ctaggcagag tcggccggcg tgatcacgac tgccgaactg caatcgttgc
     2461 aggccttggt cactcccagc ggggcagccg ccgtcaacat ggccagtcca gtccagagcc
     2521 tgacctacaa ggtcgtcgat ggcgatccgg ccaatgctca ataccagggg acggcgctcg
     2581 gcaatctcca ggcgggtaac cccgcgagcc atttgcagga gctggtcgat aagtggttcc
     2641 tggggcaaga cactcccaca atcgacatgg aatacctgtc aggctcttcg gtcagctact
     2701 cgctcgccag cggcacactc ttcggcagcg gcggccccag ctacaaagac gtgtatcaag
     2761 gcgaggaagg agactgctgg ctgctctctt cgcttgcggt cacggccgcc catgatccct
     2821 ccctcattca gagcatgttc acgagcgatg gcacccagat ggaaaacggc gttccggttc
     2881 aggtctggac ggtccggttc tacgacaacg gagtcgcaag ctatctcaca gtgaacaatc
     2941 tcctgccggc cagctccggc aacttggtat acgcaggcgg cttccagtcg atcagcaatc
     3001 cgaacaacgt cctctgggtc gaattggccg agaaggcgta tgcccagcta tgcgcgtccg
     3061 gctggaactc gcgccctgag tccaacgcct tcgcgtcatt gaacggcggc agcgcatcga
     3121 ccgccctgcc ggtgatcgca ggcgctcagg aaagcacgtc caacgccttt gcaaattcga
     3181 ccagctttat caatgcgatc gacgccggaa tgctgctcac tctcggcact tacccgagcg
     3241 gtaacaacgc ccttgggatc gtgggcaacc acgattattc ggtggtgggt tacaatgcgt
     3301 ccaaccagac attcacgctt ttgaatccgt ggggctggaa taataacaat gctccgggga
     3361 ttctgaacct cacctggacg ctgcttgcag agaacttctt cctggacggt aacggcaacc
     3421 gaccgagttc cgcttccttg ccgcagaccg gctccgattc gtcgccgagc gccggatcga
     3481 tggacacgac tgggccgatg gaggccatta tcttcagttc cagcgacgac atgggtcacg
     3541 gttcagcgaa ggcggcgaga agctattgga gcgattgagg ctccgattgc cgcacgccca
     3601 agggtcgctg gtagtcaacc ggcatgacgg accaatcaag ttttactcgt tgagtgcgtc
     3661 ggacgtttcg aggggaggat gctcgccggc tgcaacgagg agccgccgca ggtttaccaa
     3721 tcccgaacga gagcattcgc aacacccacc tgcgcggcgc caaacttctc gcttcaatcg
     3781 ctttcagcag cgactagcga gagacctctg ccaggagatg gatggtggcc atttcgtgcg
     3841 ctcgtcgtct tgacccgggg acgatgaccg gcgtcaacct gtcgcgtcat tatacaggga
     3901 gcggacgttt tgtcaccgcc cgaattcaca cagcgatcgc acgtgtgcca ggctgccatc
     3961 attggcccag ccgtcgagtt gtgcggtgtc cttgagctgc tgaccgcgct ggcgatcgac
     4021 ggcgaggaac gcacagttca gatcgggaag tgagctcatg tctccccaga gtttttcgaa
     4081 ctgggttacc gggtacacgt cttcctgtcc gtggccccag cggcgcttca cttccttgat
     4141 cgtcacataa gttggaattc gggccgccat cgcccggacg gcggcatcaa ttcgttgcga
     4201 atcggacttg agcgctccgc gcgaaagtcc aaatgcggcg agtaggccgt cgatccttac
     4261 ccaggtcgtg agcgtgttgt aatacctgag cttgaactcg gtgtcctcgc gtggctgcgc
     4321 caatccttcg agcagtcgca gccggccggc gaccttggcc agtcctccgc ctcggtcgtc
     4381 gatccgccgt ggtatgacct caaagctcag gctggcgccc gactcgacca ccatgccgag
     4441 cactgccggc gcgaccgatg cgcccagcgt gtcgatattg tgcaccaaga gccattgaag
     4501 attgggatgc tcgtcaagca gacgtgccag caaaccatta cgaagcaggt tcggcacttc
     4561 gtaaaagtgc cccggggggt taaaccgctg gatgggcacg ttgtcggtgt agtcggctcc
     4621 ttccccctgg cccttggccc agtcgaggat ggcgcggcgg ctggcgtccc gcaccttttc
     4681 cttgttgtcg tcaagcttct catggctggc ctcttcccaa agatacgaga gctcacgcgc
     4741 cattggaatc aaccgctgcc cgatcgaccg gccgcgcgaa agatacaccg agccatcgtg
     4801 tccatagttg ctcgtggacc ggaggtgacg ttcgatggcc tcgtgcgtca agtaactggt
     4861 tgtcacgatg tgcgggatct gggtcttgaa ctcgcgctgg gccttcttgg tcttggcgag
     4921 atggatttcc aggaagctgc ggtgccggcc gtcgagctcg acgaacggat tgacggcctt
     4981 gacgacgcca gcaccggttg tccatcgact gccaacgccg gcggcgagcg tcaccacggc
     5041 gacttcgccg cgctcgagcg ccgacttgcc gcgaccgaac gccgtccccg cgccaaggtc
     5101 ccgcgccagg atcagctccg aatcgtcgac gtcgcgaata tccaggtcga ccggtaatcg
     5161 gttgcgtgcc aatccgatcc ggccatgctg aaggtcgtcg cgcaattgtt cgtgctggac
     5221 cggatcgaag ccgttatcac ggcggatccg ggcggcttcg gcatcccatt cggcacgcga
     5281 cgagtcgtcg gccgagcgcg tcacgggaaa gagatgattg atggtcgtgc gaaaaacgcg
     5341 caagagctcg ctggtctgcg ggcatcggtt agcgaagtgg tcgacgtccg cccgacgatg
     5401 gtaaggaaga gtggcggtcc cctctgcgac catgcgcgga acctgaatcg tgtaataagg
     5461 cggcggcatc atcgcgcttg agtcggcgtc gagtgttgca attgtaccat ggtcgttgat
     5521 cctgaagtcg tagaccaccg gctccatcgc gaacgggagg gcgtcatcta gcgacgcttt
     5581 ggcgcgcttc atga
//
LOCUS       DATAQL010001206         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5465854, whole genome shotgun sequence.
ACCESSION   DATAQL010001206 DATAQL010000000
VERSION     DATAQL010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5465854"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1377)
                     /locus_tag="VHS97_20785"
     CDS             complement(70..1377)
                     /locus_tag="VHS97_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450704.1"
                     /translation="MTEPNPPRILSPSRRDFLKTSTAATVGLGMLGNAHAAGSDAIKV
                     GLVGCGGRGTGAAENICEAAGTSYNIKLYAMADVFEDHLKNCRDRVRDHPLCKEKFDV
                     ADERSFVGFDAYQKVIDCCDLVMLATPPGFRPQHIEATIKAGKHLFSEKPVAVDGTGI
                     RKVLAAYDEANKKGLSVITGTQRRHQAGYLESMKRIHEGAIGDLLSARVYWNQGDIWA
                     HKRQPGWSDTEYQIRNWYHFLWLCGDHIVEQHVHNLDVACWALKAHPLKAVGMGGRQV
                     ITEPERGQSYDHFAIDYEFPNGVHVLSMARQIPGSAGNVSETLVGTKGRWHSKGSPES
                     QRAYLFTGPNAESLRIHEVNPYVQEHIDLLESISSRKPVNELKQVAESTLTAIMGRMS
                     AYTGKAVSWDQALNSKLDTFPKTLAWCPMEEPPIPKPGVTELI"
     gene            complement(1477..>1887)
                     /locus_tag="VHS97_20790"
     CDS             complement(1477..>1887)
                     /locus_tag="VHS97_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX3450705.1"
                     /translation="NNAEKPMPIGKKKPNPWGLYDIHGNVAEWCLDHYVADAYKPYPT
                     DKPTLGPVILPDAKEYPYVARGGSWDDDAPLLRSAARRASNLEWSVQDPQRPQSIWWH
                     TDATFVGFRVVSPLNEQANLQGLKSPIVKGKTTR"
BASE COUNT          322 a          543 c          600 g          422 t
ORIGIN      
        1 aaatcatcgt cccgcggtcg gattgtccaa tcgatcgagt cgttgccagc ggctcgcatt
       61 cccatcggct cagatcagct cggtcacgcc cggcttggga atcggcggct cttccatcgg
      121 gcaccaggcc aaggttttgg ggaacgtgtc gagcttggag ttgagagcct ggtcccatga
      181 cacggccttg cccgtatacg ccgacatccg gcccatgatg gccgtcaacg tgctctcggc
      241 gacttgcttg agctcgttga caggcttgcg ggaggagatg ctttcgagca ggtcgatatg
      301 ctcctggacg tagggattga cttcgtggat gcgcaagctc tccgcgtttg gacccgtgaa
      361 aagataagct cgttgacttt cgggcgagcc tttgctgtgc caccggccct tggtgccgac
      421 gagcgtttcg gagacattac cggcgcttcc cgggatctgc cgcgccatgc tcagcacgtg
      481 cactccgttg gggaactcgt aatcgatggc gaagtgatcg tagctctggc cgcgctcggg
      541 ttccgtgatc acctgacggc cacccattcc cacggccttg aggggatgcg ccttcagtgc
      601 ccagcacgca acgtcgaggt tatgaacatg ttgctcgacg atgtggtcgc cgcagagcca
      661 gaggaagtgg taccaattgc ggatctggta ctcggtgtcg ctccagccag gctggcgttt
      721 gtgggcccag atgtcgccct ggttccagta gaccctcgca gacaagaggt cgccgatggc
      781 cccctcatga atccttttca tgctttcgag gtagcctgcc tggtggcggc gctgggtgcc
      841 ggtaatgacc gacagcccct ttttgttggc ttcgtcatag gccgccagca ccttgcggat
      901 gccggttccg tcgacggcga cgggtttctc ggaaaagaga tgcttgccgg ccttgatcgt
      961 ggcttcgata tgctggggac gaaagccggg cggggtcgcc agcatgacca gatcgcaaca
     1021 gtcgattacc ttctggtagg cgtcaaagcc gacaaacgat cgctcgtcgg ccacatcgaa
     1081 cttctccttg cagagcggat gatctcggac gcggtcgcgg cagtttttga gatggtcttc
     1141 gaagacgtcg gccatggcat agagcttgat gttatagcta gtgcccgcgg cttcgcagat
     1201 gttttcggcg gcgccagtac cgcgcccgcc gcagccgacc agaccgacct tgatcgcgtc
     1261 gctgccggcg gcgtgggcat ttcccagcat tcccaggccg accgtggcgg cggtcgatgt
     1321 cttcaagaaa tcacggcgcg acggagacaa gattctcgga gggttcggtt cggtcatgat
     1381 tcattctcct ggagttggga agagcctcgt tcacgcggtc actcacttac ttgctcgggc
     1441 cgtcggtcgg acccgagggt ttcgattcct ggcgaatcaa cgtgtggtct tgcccttgac
     1501 gattggcgac ttgagtcctt ggaggttcgc ctgctcgttg agcgggctga cgacccggaa
     1561 accgacgaat gtcgcgtcgg tgtgccacca gatgctttga ggtcgttgcg gatcttgaac
     1621 gctccattcg agattggaag cgcggcgggc agccgatcga agcaggggcg catcgtcgtc
     1681 ccaggaaccg ccgcgggcca cgtaggggta ctctttggcg tccggcaaga tcacggggcc
     1741 gagagtaggt ttgtccgtcg gatacggctt gtaagcatcg gcgacgtagt ggtccaggca
     1801 ccactcggca acattgccgt ggatgtcata aagaccccac ggattcggtt tcttcttgcc
     1861 gatcggcatc ggcttttcgg cgttatt
//
LOCUS       DATAQL010001207         5057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3330071, whole genome shotgun sequence.
ACCESSION   DATAQL010001207 DATAQL010000000
VERSION     DATAQL010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330071"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VHS97_20795"
     CDS             complement(<1..521)
                     /locus_tag="VHS97_20795"
                     /inference="COORDINATES: protein motif:HMM:NF039752.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450706.1"
                     /translation="MTIGIPCRPRRHGSVAAATALYIPSRDLNVLFAQCGRLGLDPTG
                     RLFDVAGGFLLELLAPSIDPAPGAVRLRALHRWLYLPVDSELVPGLLDDEASGLVRDW
                     GLIFLPGGRTLLYDRHAPIELTELLRAQSEAERVRGRAWSSFPEAGQLADRLVQIAME
                     LPPEPPETVYNSF"
     gene            complement(518..3379)
                     /locus_tag="VHS97_20800"
     CDS             complement(518..3379)
                     /locus_tag="VHS97_20800"
                     /inference="COORDINATES: protein motif:HMM:NF039750.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450707.1"
                     /translation="MSEDRAALYLQIPATYCRSFGGLRWAQYGEAVEFLEGPAAGRTF
                     AFAAEIAVFLEGVQDPGGSILGFGFVLHLLYLIGLGDRAARHGEGCALCIERIAAPFR
                     ELGCPLRNAGALCSWLSRAAPHTADPPDLTELHEILTSGSWVPQMVLSHRLRGVMDQA
                     EEPGLVSDEFEEMVRRAAELLSDGEIRHWLRHGRGTVGTADLRLLPDRPRNLVEVLIN
                     LERSPRLAGIGRLVTSLEGAISLPPRRLAWAVLQDGGYADVTTKGAPEQILPIQFALD
                     DEEFLRRFAERELLYFHREEPRQPTTEEIVLLLDQGVRTWGDVRLVLAGAVIALARQA
                     QRRRIAIKLATTSNGGEAIDPVQLEPRALRALVETSDLSPHPGTALLHLLDSPAAAAP
                     RDIVLLTHPRNLGEPAVRDAARSLPEEAGSRLFAISVDASGQLELAELRRGLPVALGR
                     SRINLELATAAALTPSSSPYLAQRTAWQGDVESIPFPFKCGTLDRIDAPRNDDHTHLD
                     FDEAGERILVIGRHGLLFTWRIDGTDLEILPRPVVHGEVSRNPTTAIGVAGGFVVVSR
                     RQGRLLLAHYDFPTRTCRVHTHFDSEAAESWVYYRDLHCVVARRETPEVSSPALDLGA
                     PVGRAAATTRSRLATKRAQKGVAPEPLIVREEVSRFLIDSWRKPFSTAVNLDPKNGTL
                     NVHQSTGENWSLTPLRDGRPALERGNILQARQAADVLAVLVERGTAPGLYFLSLSRAV
                     VLGTDYHPSDEFGTSVFALSRDGQRFARLIDDRRLDVRDVPGSHPPVLVTPVESLWMH
                     FATLGKSCLLVREFDVTGTRRARSCCLIRWDRGLLEVNDRGAEALLEHLGGTVAVSRA
                     VVPAMRTPGQDPNRFVQFIAHEGARILIDRYNHIAVLTKSGALTCMFYVNGNDVAAWL
                     PDGTCWGSRRLIGGEPSAAAAERIAAVLQSTFDDEGSSP"
     gene            complement(3376..4197)
                     /locus_tag="VHS97_20805"
     CDS             complement(3376..4197)
                     /locus_tag="VHS97_20805"
                     /inference="COORDINATES: protein motif:HMM:NF039751.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450708.1"
                     /translation="MSTADITARWKDICGASLRVEDLASLADLRGRAQIRVTLAGGRA
                     WVWWQADSNVVPELVARRIMPLEGVELFTRTAGQWYRLGERLPAFGVPFAVGGAGASL
                     DRIVVPAKISAERPAGNLPERLAVRVVPCDSRQNRPATALRASIDELAAWADWATSFR
                     LSCLRAARRAAAREEEGPSEALVLGDPGTLPPLPGSERYWGADLLVPLGFRSDPELPA
                     SAIRHIVGAGQDELVLMDEKGYELIARKAFQPLTRSGIRLAREGARSGPEKGSPK"
     gene            complement(4448..4819)
                     /locus_tag="VHS97_20810"
     CDS             complement(4448..4819)
                     /locus_tag="VHS97_20810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450709.1"
                     /translation="MLVADDNLAIVRTTALLLQYAGFEVRTASNGHDALKVAREFHPQ
                     IALLDVGLPGIDGYEVVRRLRADLTLQMMTLIAITAYGTEEDRHRAKAAGFDHHLVKP
                     VHFYNLLSVMGPRSRPRGHLQ"
BASE COUNT          997 a         1680 c         1583 g          797 t
ORIGIN      
        1 ttgaacgagt tataaaccgt ttccggcggc tcaggcggca gctccatggc aatctgtacg
       61 agtcggtcgg caagctggcc cgcctcggga aacgagctcc acgcgcggcc gcgcactcgc
      121 tctgcctcag attgagcgcg caagagctcg gtgagctcga tcggagcgtg acgatcatag
      181 agcagtgtac ggccgcccgg cagaaatatc aggccccaat cgcgcaccaa gccactggcc
      241 tcgtcgtcca gaagaccggg aaccagctcg gagtcgaccg ggaggtacag ccagcgatga
      301 agggcacgaa ggcgcaccgc accgggggcc gggtcgattg acggcgctag gagctcgagc
      361 aaaaacccac cggccacgtc aaagagacga ccggtggggt cgaggcccag ccggccgcat
      421 tgcgcgaaca acacattcag gtcacgcgac ggaatataga gggcggtcgc ggctgcgacc
      481 gatccgtggc gccgcggccg gcacgggatc ccgatcgtca tggcgaactc ccttcgtcgt
      541 cgaaggtcga ctgaagcacc gccgcgatcc gttcggcggc ggcggccgaa ggctcgccgc
      601 cgatcaggcg gcgcgagccc cagcatgtcc catcgggcaa ccaggcggca acatcattgc
      661 cattcacgta gaacatgcag gtcaaagccc cgcttttcgt tagcacggca atatgattat
      721 accgatcgat caggatccgc gcgccctcgt gcgcgatgaa ctggacgaat cgattggggt
      781 cttggccagg cgtccgcatc gcaggcacaa ccgctctcga caccgcgacg gtccctccca
      841 ggtgttctaa gagcgcctcg gcaccgcgat cattcacttc gagcaagccc cggtcccaac
      901 gaatcaggca gcatgagcgg gcacgccgcg tcccggtcac atcgaactcg cgcacgagca
      961 ggcacgactt ccccagagtt gcaaaatgca tccataagct ctcgaccggc gtgaccaaga
     1021 ctggcggatg tgagccgggc acgtcgcgca cgtctaaccg gcgatcgtcg atcaagcgcg
     1081 caaatcgttg gccatctcgg gacagggcga atacgctggt gccgaattcg tccgacgggt
     1141 ggtaatcggt gccgagaacg acagcgcgcg acagcgagag gaaatagaga ccaggcgcgg
     1201 tccctctttc aaccagcacc gccaaaacgt cggccgcctg gcgcgcttgc aggatgttac
     1261 ctcgctcgag cgccggacga ccatcgcgca ggggcgtcaa cgaccagttc tcgcccgtcg
     1321 attgatgaac gttcaaagta ccgttcttgg gatcgaggtt cacagccgta gaaaagggct
     1381 tcctccatga atcgatcagg aaccgcgaaa cctcctcccg cacgatgagc ggctcgggcg
     1441 cgactccctt ctgggcgcgc ttcgtcgcga gtcgcgaacg tgtggtggca gccgctcggc
     1501 cgaccggcgc acccaggtcg agggccgggc tgcttacctc tggggtctcc ctccgcgcca
     1561 ccacacagtg caagtcgcga tagtaaaccc acgactccgc ggcctcgctg tcaaagtgcg
     1621 tatgaaccct gcaggtccgg gtcgggaaat cgtaatgggc cagcagcagt cgtccctgac
     1681 ggcggctgac cacaacaaag ccgccggcaa cgccgatcgc cgtcgtcgga ttgcgagaga
     1741 cctcaccgtg gacgaccggg cgcggcagaa tctcgagatc ggttccatcg atccgccacg
     1801 tgaagagcaa gccatgacgg ccaatcacca ggattcgttc gccggcttcg tcgaaatcga
     1861 ggtgagtgtg atcgtcgttc cgcggtgcgt cgatgcgatc gagcgttccg cacttgaacg
     1921 gaaaagggat cgactcgacg tctccctgcc aggccgttcg ctgggcgaga tatggcgacg
     1981 acgacggcgt aagggcggcc gcggtcgcca gttcaaggtt gatgcggctg cgtcccagtg
     2041 cgaccggcaa accgcgccgc agctcggcca gctcgagctg ccccgacgcg tcgaccgata
     2101 tcgcgaacaa ccgagagccc gcttcttccg gcagcgaacg cgccgcgtcc cgaactgccg
     2161 gctctcccaa gttgcgcgga tgggtcaaga gcacaatgtc tcgcggcgca gcagcggcgg
     2221 gtgaatccag caggtgcaag agagcagtac cgggatgcgg cgacaggtcg ctcgtttcca
     2281 ccagcgctcg caaggcgcgg ggctcgagct ggaccggatc gatggcctcg ccaccgttcg
     2341 atgtggtcgc cagcttgatc gcgattcgcc gccgctgggc ctgccgggcc agcgcgatca
     2401 ctgccccggc gagcaccagc cgcacgtctc cccaggtgcg caccccttgg tcgagcaaga
     2461 gcacgatctc ctcggtcgtc ggctgacgcg gttcctcgcg atggaagtat aagagctcac
     2521 gctcggcaaa ccgtctgaga aactcctcat catcgagagc aaactggatc ggcaagattt
     2581 gctccggggc ccccttggtg gtcacgtccg catagccgcc gtcctgcagc acggcccagg
     2641 cgagccggcg cggcggcagc gaaatcgctc cttcgaggct ggtcaccagc cgcccgattc
     2701 ccgcgagccg cggacttcgc tccagattga tcaacacctc cacaagattt cggggacgat
     2761 cgggtaagag ccgaaggtcg gccgttccca ccgtgccgcg accgtggcgt aaccagtggc
     2821 ggatctcgcc gtcggaaagc aattccgccg cgcgtcggac catctcctcg aattcgtccg
     2881 acacgagccc aggctcctcc gcctggtcca tcactccccg cagccggtgg ctgagaacca
     2941 tctgaggaac ccagctcccg ctcgtcagga tctcgtgcag ctcggtcagg tcgggagggt
     3001 cggcggtgtg aggagccgcg cggctgagcc acgagcacaa agccccggca ttgcgcaacg
     3061 ggcagcccag ctcacgaaat ggcgccgcga tccgttcgat gcacagggca cagccttccc
     3121 catgccgggc cgcgcgatca cccagaccga tcaggtagag aagatgcagc acaaacccaa
     3181 agccgagaat cgacccgccg gggtcctgca ccccctcgag gaagaccgcg atttcggcgg
     3241 cgaaggcaaa ggtacgcccc gccgccggtc cctcgagaaa ctcgaccgcc tcgccatact
     3301 gcgcccatct cagcccgcca aatgaccggc agtatgtggc gggaatctgc agatagaggg
     3361 cggcgcggtc ctcgctcatt tcggactccc tttctcgggc ccggatcgcg cgccctcgcg
     3421 tgcgagccga ataccgctgc gagtgagcgg ctggaacgcc ttccgcgcga tcagctcata
     3481 gcccttttca tccatcaaaa ccagttcatc ctggccggcc ccgacgatat ggcgaatggc
     3541 ggatgcgggc agttcgggat ccgaccgaaa accgagcggg accagcagat cggcccccca
     3601 gtaccgctcg ctgccgggca aaggcggcaa cgtgcccgga tcacccagca ccagagcctc
     3661 cgacgggcct tcctcttctc gggcagcagc gcgccgggct gctcgtaaac acgaaagccg
     3721 aaatgaagtc gcccagtccg cccaggcggc gagctcatcg atcgaagcgc gcagtgcggt
     3781 ggcggggcga ttctgtcttg agtcgcaagg gaccacgcgg accgccaatc gttcgggcag
     3841 gttacccgca ggccgctcgg ccgaaatctt ggcgggaacg acaatgcggt ccagggacgc
     3901 gccggcgcct ccaacggcaa acggcactcc gaacgccggc agccgctcac ccaggcgata
     3961 ccactggcca gccgtccgcg taaacagctc gaccccctca agcggcatga tccgtcgcgc
     4021 caccagttca ggcaccacat tcgaatctgc ctgccaccac acccaggcac ggcccccggc
     4081 cagcgtcacg cgtatttgag cgcggcctct cagatcggcc agcgatgcca agtcctcgac
     4141 gcggaggctg gcgccgcaaa tgtctttcca gcgtgcggtg atgtcggcgg tgctcactgg
     4201 agatggcctt tcaggatggg gcgcgatcgt ttcatcatcc caggtcccac tcaagcgtaa
     4261 atagctcagc ttcgacgtgg aaggtgctgg attgagagat caagcgccgc gatgcgaaaa
     4321 ccaagcgctc ccacggaacg tgatcagctc tcgagaacct caaacagccg taaccgccca
     4381 cagcctactt gtccaaccca cgcatcgccc tgtaccgggc gacccgccaa ttgtggagct
     4441 cttcaactta ctgaagatgt ccgcggggtc gggaccgggg gcccataacg gagagaaggt
     4501 tgtagaagtg cacgggcttg acgaggtgat ggtcgaatcc ggccgctttg gcacggtgcc
     4561 gatcctcctc ggttccgtag gcggtgatcg cgatcaaggt catcatctgg agcgtgagat
     4621 cggcacgaag ccgacggacg acctcgtagc cgtcaatgcc ggggagcccg acatcgagca
     4681 gggcgatctg cggatgaaac tccctagcga ctttaagggc gtcgtgtccg ttggacgcgg
     4741 tccgcacctc aaaaccagca tactgcagca acagagcagt tgtgcgcacg atagcgaggt
     4801 tatcatctgc gacgagcacg cggagtctct cagttggcgg ctcgtggttg gggagacttg
     4861 ggtctgtgtc gtgttcacca accatggatg ccccctgttt gtccgtcatc gcccacaggt
     4921 ccgaatggca agacttcgcg ctggacagac aaccggaggg acaggcgttt gctccaaatc
     4981 gggagggcca tgctggccta ctcgccggcg gccggcgttc cggggtcggt cgccggctcg
     5041 tcagggcata gtgtcag
//
LOCUS       DATAQL010001208         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5007685, whole genome shotgun sequence.
ACCESSION   DATAQL010001208 DATAQL010000000
VERSION     DATAQL010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5007685"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VHS97_20815"
     CDS             <1..502
                     /locus_tag="VHS97_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450710.1"
                     /translation="DAGRLDEAFPDLEAILNHPGLVEHLRKEPMLLARMHGPTESLIL
                     NLHGASRRYCLGGKFDKGRTIARRALEVAILLNRHRAASHYNLAKVHALAAPIEPNYI
                     VNAAKQLYLAFLANPVYRRDYYEQDPLFDVVRAQIDAQLDQKPDPSEHYHRRMAANSV
                     GKDPSH"
     gene            complement(544..1296)
                     /locus_tag="VHS97_20820"
     CDS             complement(544..1296)
                     /locus_tag="VHS97_20820"
                     /inference="COORDINATES: protein motif:HMM:NF016361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 C-terminal domain-containing protein"
                     /protein_id="HEX3450711.1"
                     /translation="MGEESYLIRYGVMSHVGRFPALPTCDHSLERGQLVVIQTDRGLE
                     LGEVLIATNGTVALSGNRPYGATAHSDGDDSMPHVSPSSPHVLRAAGPDDLSRARSAL
                     AARSGRFSLCQRILEEGNWPWELVDVEPLLDGQATVLHYLGPHKIDAAPLRARFRVEC
                     AIDVVLEPVGEDVGPEFSEEDADPEDPGGCGSCGCGEGGGCGKTRGAAAEPVSHGHGA
                     ETAVTACATSSHSGCSSCGIGRLLADRRHATA"
     gene            1710..>2081
                     /locus_tag="VHS97_20825"
     CDS             1710..>2081
                     /locus_tag="VHS97_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX3450712.1"
                     /translation="MSEARCTLQVRGLDCPKEVDSLTAALKDQSGVTRMGFDLIHGTM
                     TIDYDDGLIDPQTFIRLIDERAGLQSSLQGLVEPAAISWWSRYGRWVLTAGSGAALAG
                     GTVISWMGHRLGLEQAGADRLA"
BASE COUNT          419 a          668 c          607 g          387 t
ORIGIN      
        1 cgatgccggc cggcttgacg aggcgttccc agacctcgaa gccattttga accaccctgg
       61 actcgtagag catctccgca aagaacccat gctgctcgcc cgcatgcacg gcccgaccga
      121 gtcgctcatc cttaacctgc acggcgcgtc gcgccgctac tgtctcggcg gcaagttcga
      181 caaaggacga acgatcgctc gccgggcact cgaagtcgcg attcttctca accgacaccg
      241 cgctgcatcc cattacaacc tggcaaaggt ccacgccctc gcggccccga tcgagccgaa
      301 ttatatcgtg aacgccgcca agcagctcta cctggccttc cttgcgaatc cagtgtatcg
      361 gcgcgattat tacgagcagg acccattgtt cgacgtggtc agggcgcaga tcgatgccca
      421 actcgatcag aagcccgacc cgagcgagca ttatcatcgt cgtatggccg cgaattcggt
      481 tggtaaagac ccgtcgcact gagcggtcgc tctcgcggtc tcgaattcgc gcttgtgctt
      541 atctcaggcg gtcgcatgac gccgatcggc cagcaatcgc ccgattccgc acgaggaaca
      601 gcctgagtgc gatgaagtgg cgcacgccgt gacggccgtt tccgcgccgt gtccatggct
      661 gacaggctca gcagccgccc ccctggtctt gccgcagccg cccccctcgc cgcatccaca
      721 cgatccgcag cccccggggt cttccggatc agcgtcttcc tccgagaatt cggggccgac
      781 gtcctcaccg actggttcca gtaccacgtc gatggcgcat tcgactcgga accgcgcacg
      841 tagaggcgcc gcgtcaatct tatgcgggcc gaggtaatgg agaaccgtcg cctggccatc
      901 gagaaggggc tcgacatcga cgagctccca tggccaattc ccctcctcga gaattcgttg
      961 acagagggaa aagcggccgg aacgagcggc tagggcgctc ctcgcacgag ataggtcgtc
     1021 ggggccggcg gcgcgcagca cgtggggcga gctcggtgaa acatgcggca tcgagtcgtc
     1081 gccatccgag tgagccgtag cgccgtacgg tctattaccc gacagcgcca ccgtgccgtt
     1141 cgtggcgatc aacacttcgc ccagttccag ccctcgatcc gtctggatca ccaccagctg
     1201 gccgcgttcg agggaatgat cgcacgttgg gagagcagga aaccggccca cgtggctcat
     1261 gacgccatag cggatcaaat acgattcttc gcccaccgcc cctccccacg tccatgggtt
     1321 gtcgcaggtg tgtgtcgact tgcgtcgact tttcctccca gtttatcgac atgcctatca
     1381 gaaacaaacc ggagggtggc cacaagaccg ttccgcgtaa acgaattgcg agacgaggtc
     1441 tgaaaaaccc gtatcaaaaa gaatcgcccg cccgatacca tacgaacgcg agccagcgga
     1501 tccccgaggg aacgtgcaac aacgcagacc ccttcaagac ggactggccg gatcgaaaac
     1561 cacgcttgaa cgtgttcacg caacgtggcc tctcgcatat aattacaagg agattgaggg
     1621 ctctggccga tcggcgaaat cgacggttgt tagctaaccg tcttgcggtc accctcgccg
     1681 ttgaatggac ttggctctag gatgttagga tgagcgaagc tcgctgcacg cttcaagttc
     1741 gcggccttga ctgtcccaag gaagtcgatt cgctcaccgc cgcgctgaag gatcagtccg
     1801 gcgtcacccg catgggcttc gacctgattc acgggacgat gacgattgat tacgacgacg
     1861 gactgatcga tccgcaaaca ttcattcgct tgatcgatga gagagccggg ctgcagtcaa
     1921 gtctgcaagg gctggtagaa cccgccgcaa tatcatggtg gtcgcgatat gggcgctggg
     1981 tcttgacggc cggatctggc gcggccctgg cgggcggtac cgtgatttcc tggatgggcc
     2041 atcggctcgg ccttgagcaa gctggtgcgg atcgcctggc a
//
LOCUS       DATAQL010001209         5669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3330134, whole genome shotgun sequence.
ACCESSION   DATAQL010001209 DATAQL010000000
VERSION     DATAQL010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330134"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VHS97_20830"
     CDS             complement(<1..1122)
                     /locus_tag="VHS97_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450713.1"
                     /translation="MTTHALSAESASSPERPLTRRDLLAKAGGGCGLLGLAALIAGEQ
                     PLSSAARADESRTNLALNPMAPRPTHFAPKAKRVIWIFVNGGPSQVDTWDYKPALTRW
                     NGKSMNQFDPSFQNTTGFFKDQVGALMQSPFRFSPQGQCGKMVSELFPNLGPHVDRMA
                     FIHSAYSESNNHSPALFMMNTGMSRMGFPCVGSWVTYGLGSESQNLPGFVVMSDPRGR
                     GLPKGHAANWGAAFLPGAFQGTHLHSSGSPIDNLDRPGEMPPPRQRAQLYLVKRLNEL
                     HREQFAAEAELTARIESFELAYRMQSAAPEAISIESESEHIKTLYGFDDPRCEHVARQ
                     CLTARRLVERGVRFVQIYSGGMENQLSWDGHADIKGNHQQ"
     gene            complement(1144..3402)
                     /locus_tag="VHS97_20835"
     CDS             complement(1144..3402)
                     /locus_tag="VHS97_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX3450714.1"
                     /translation="MQASFTSVICWGIEALFFTVFLAPAPASAQEEKKAVPADHPERM
                     RQGLVLFKEHVRPALVQHCLMCHGGKMTKGKFDLSDRGPLQKSGAIDGGGKESRLYAL
                     ITHAEEPHMPQKGPKVSTATADQIARWIDLGAPYDAPLVERPAAAAARSPALDRDFWS
                     FRPLQDPKPPSVQNAEWIRTPVDRFILAELEAHGLAPAAPADRRTLIRRVYFDLIGLP
                     PTPDEVDAFVADPDPSAYLRLVDRLLASPHYGERWARHWMDVARFAESHGYEQDYDRT
                     HAYHYRDFLIRALNRDMPYTQFAAWQVAGDELAPADPMALAATGFLGAGAFPTQLTEA
                     EFESARYNELDDVVSTIGTAFLGLSVGCARCHDHKYDPIPSDDYYRLAATFTTTIRSE
                     IDVPLGPGSKPVKMQVTSEGFPHTKHHADERGFPHFYKETYILRRGDVNQKAGEATPD
                     VLRVLNRGGRNMSFWKVETPEGWSRTSFRRAALTRWLVDTEAGAGQLIARVAANRVWQ
                     HHFGRGIVATPNDFGAQGAAPTHPALLEWLASELVARGWHLKALHRVIVTSAVYQQSD
                     QFQTTHEAIDPDNAYYWRYTPWRLEAEPIRDTMLAIAGLLDQRMYGPGSLDPGMSRRG
                     VYFFIKRSQLVPMMMLYDWPEHLVSIGQRSQTTTAPQALVFMNSPLGRRCATGLADRL
                     AFLPSEAAIRHGYRFVLGRQPTQDEARLSADFLAAQANGYAQAGRADPNHLALVDFAQ
                     ALLSTNEVIYVP"
     gene            3831..4154
                     /locus_tag="VHS97_20840"
     CDS             3831..4154
                     /locus_tag="VHS97_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450715.1"
                     /translation="MLNAKMPHLVLDKQVPAQVFDLKQQKNTGSKVVGPSVTVLGHVQ
                     AGHYHFTNLDGPNAGTNAGSGTNENGIANNGTAVGFTIDNNGNFLNFTANPVKSKKAT
                     LVNIK"
     gene            4167..4841
                     /locus_tag="VHS97_20845"
     CDS             4167..4841
                     /locus_tag="VHS97_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450716.1"
                     /translation="MAFGVNSRGTVVGTDGNGNAFFAQGNKVTTFIPAGGMSATAFGI
                     NDKSMIVGQYVTSTATPGFIRLNSKSYVTINAPSGTNVVNAQGINNKGQVVGFYLGTD
                     GQVHGFTANANGSKAGSITGVAIADPTIPVVPGEPGATFVFSQILGINDKGIAVGYYG
                     DSTTSQHGFLYNTHTGVYTFLDDPSEAFNNGVEVTQITGITNSGEISGFYSDANGVFH
                     GFVATA"
     gene            5068..>5669
                     /locus_tag="VHS97_20850"
     CDS             5068..>5669
                     /locus_tag="VHS97_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450717.1"
                     /translation="MASGLETRGQVARTMAGTEGVEIKATIPESQVELALQIYGLNLD
                     DNERYIYFFDTPELELFAEGIIARARRIVGAAHDSTIKFRPVDPESVPALWRKYSGFK
                     IEADASHKGVVKSASLTMPVAKGQIKRVAAGSAPVASLFAEEQLLFLLSMANKKIDYT
                     KVVVMGPLRAWRWKYIDPGLPWPITGELWQRSDGARLFEVS"
BASE COUNT         1109 a         1671 c         1823 g         1066 t
ORIGIN      
        1 ctgctggtgg ttgcccttga tatcggcatg gccatcccag gagagctgat tttccatgcc
       61 gcctgaatag atctggacga agcggacgcc ccgttcgacc agacggcggg cggtcaggca
      121 ttggcgggcg acgtgttcac agcgcggatc gtcaaagccg tacagcgttt tgatatgttc
      181 agactcggat tcgatcgaga ttgcctcggg agcggctgat tgcattcgat aggccagctc
      241 gaagctttcg atccgagccg tcagctcggc ctccgcggcg aactgctcgc ggtggagttc
      301 attcagacgc ttgaccaggt agagctgcgc tcgctgccgc ggcgggggca tctcgccggg
      361 gcgatccagg ttgtcgatgg gtgagccgct cgagtgcagg tgggttcctt gaaatgcacc
      421 cggcaggaac gcggcgcccc agttagcagc gtgacctttg ggcagcccgc gcccgcgcgg
      481 gtcgctcatc acgacgaatc cagggaggtt ttggctttcg ctgccgaggc cgtaggtcac
      541 ccatgagccg acgcagggaa accccatgcg cgacattccc gtattcatca tgaacagggc
      601 aggggagtgg ttgttcgact cgctgtaggc ggagtgaata aacgccatcc ggtcgacgtg
      661 aggacccagg ttgggaaaca gctccgagac catcttgccg cactggccct ggggactgaa
      721 acgaaaggga gactgcatca aggcgccgac ctggtctttg aagaacccgg tggtgttttg
      781 aaacgacggg tcgaactggt tcatcgactt gccgttccag cgggtgaggg ccggcttgta
      841 atcccaagtg tcgacctggc tcggaccgcc gttgacgaag atccagatga cgcgtttggc
      901 cttgggcgcg aagtgcgtcg gccgcggggc cattgggttc aaggcaagat tggtgcggct
      961 ctcatcggcc cgcgccgctg atgaaagcgg ctgctcgccg gcgatcaaag cagccagacc
     1021 caggaggccg cagccgccgc cggccttggc aagtaagtcg cggcgcgtga gggggcgttc
     1081 tggcgaggac gccgattcag ccgaaagagc gtgcgtcgtc atcggtggtc ctcactcgtc
     1141 ggatcaaggg acatagatta cttcgttcgt gctcaaaagg gcctgggcga aatcgacaag
     1201 tgccagatgg ttggggtcag cgcggccggc ctgggcgtaa ccattggcct gagcagcgag
     1261 gaaatccgcc gagagccgag cttcgtcctg ggtcggctgg cggcctaaaa cgaatcggta
     1321 gccgtgccgg atcgcggcct cggacggaag aaaggcgagg cggtcggcca agcccgtggc
     1381 gcagcggcgg cccagcggac tgttcatgaa caccagggcc tgcggcgcgg tcgtcgtctg
     1441 gctccgctgg ccgatgctca ccaaatgctc cggccaatcg tagagcatca tcatcggcac
     1501 gagctggctg cgcttgatga agaaatagac gccccgtcgt gacatgccag gatcgagcga
     1561 gcctggtccg tacatccttt ggtcgagcag gcccgcgatc gccagcattg tgtcgcggat
     1621 cggctcggcc tcgaggcgcc aaggggtata gcgccaataa tacgcgttat caggatcgat
     1681 ggcttcatgc gttgtctgga actggtcgga ttgctggtag acagcgctcg tcacgatcac
     1741 tcggtgcaaa gctttcaagt gccagccgcg ggcgacgagc tcggaggcga gccactccaa
     1801 gagggccggg tgcgtgggcg cggctccctg ggcaccgaag tcgttggggg tggcgacgat
     1861 tccgcggccg aaatggtgct gccagactcg gtttgccgcc acgcgtgcga tcagttggcc
     1921 cgcgcctgct tcggtatcga ccagccaccg cgtcagagct gcgcggcgaa agctggtgcg
     1981 cgaccagcct tcgggcgtct cgaccttcca gaacgacatg ttccggccgc cgcggttcag
     2041 aacgcgcaaa acgtcgggag tggcttcgcc ggccttttga ttgacatcgc cccgccgcag
     2101 gatataggtc tccttataaa agtgggggaa gccgcgctcg tcggcatgat gcttggtgtg
     2161 cgggaagccc tcgctggtga cctgcatctt gactggcttt gagccgggcc cgagcgggac
     2221 gtcgatctca ctgcgaatgg tcgtggtgaa cgtcgccgcc agccggtagt agtcgtccga
     2281 ggggatcggg tcgtacttgt gatcgtggca gcgggcgcag ccgaccgaca agccgagaaa
     2341 agcggtgccg atcgtggaaa cgacatcgtc gagctcgtta taacgggccg attcaaattc
     2401 ggcctcggtc agttgggtag ggaacgcgcc tgcgcccaga aagccggtgg cggccagcgc
     2461 catcgggtcg gccggcgcca gctcgtcgcc agccacctgc caggcggcaa actgggtata
     2521 gggcatgtct cgattgagcg ctctgatcaa gaaatcgcga tagtgatatg cgtgggtgcg
     2581 gtcgtagtct tgttcatagc catgtgactc ggcaaaccgc gccacgtcca tccagtgacg
     2641 tgcccaccgc tcgccgtaat ggggtgaggc gagcaggcgg tcgaccagcc gtagataggc
     2701 gctgggatcg gggtcggcga cgaaggcgtc gacctcatcg ggagtgggag gcaagccaat
     2761 caggtcgaaa taaacacggc ggatgagcgt tcggcgatcc gccggcgcgg cgggtgcaag
     2821 cccgtgagcc tccagctccg cgaggatgaa gcgatcgacg ggtgtgcgga tccactcggc
     2881 gttctgaacg gatggcggtt tcgggtcttg cagtgggcga aacgaccaga agtcgcggtc
     2941 cagggccgga gatcgagcgg ccgcggcggc cggccgctcg accagcggtg catcgtacgg
     3001 ggcgcccagg tcgatccacc gcgcgatctg atcggccgtc gcggtagaga cctttggccc
     3061 cttctggggc atgtgcggct cttcggcgtg agtgattaga gcatagagcc ggctctcctt
     3121 gccgcctcca tcaatcgcgc cgctcttttg caatggacca cgatcggaaa gatcgaattt
     3181 acccttagtc atcttgccgc cgtggcacat cagacagtgt tgtaccaagg ccggtcgcac
     3241 gtgttccttg aataatacca ggccttgacg catccgttcc ggatggtcgg caggcacggc
     3301 ctttttctcc tcctgggccg aggcgggcgc cggcgccaag aataccgtga aaaaaagtgc
     3361 ctcgattccc caacaaatga ccgaggtgaa tgatgcttgc ataacggata ccgcggctcg
     3421 ctctactggg tttgccccga tttggattcg ctcgctaatt tgcgctgacg attcgccctg
     3481 ctaggcggcg cgcccctgcc gggcttcacg ctagtgattc taagcaatcc gtccgccacg
     3541 ggctagaaac gaccgtgtca gatagagtcc acccaggggc ggccgatcga tgggccgacg
     3601 tgagaccgtt gccgtccgtt gtggcccccg cctcgggaaa atattctttc ggaagtgtgt
     3661 ccgaaaaccc tgtcgcttgg caatgtcctg gtacaaccgg agcgaaatgt ggtcgcccgc
     3721 cacactccag gtcgcaccgc acactgggag acaattgcat gtttgcttcg acacgatcca
     3781 accgccgcag gaccgctcgc cccgttctgg actcgctgga agggcgtacc atgctcaacg
     3841 ccaagatgcc gcatctcgta ctcgataagc aagttcctgc gcaggtgttc gacctgaaac
     3901 agcaaaagaa taccgggtca aaggtcgtcg gcccgagcgt tacggttctc ggtcacgtgc
     3961 aggccggcca ctatcatttc accaatctcg acggtccgaa tgcgggcacc aatgcggggt
     4021 ccggcaccaa tgaaaacggc atcgcgaaca acggtaccgc ggttggattt accatcgaca
     4081 acaacgggaa cttccttaac ttcacggcta atcccgtcaa atccaaaaag gccacgttgg
     4141 tcaacatcaa gtgatcgacg aaggccatgg ccttcggggt caactcgcgc ggcacggtgg
     4201 tgggtaccga tggtaacggc aacgctttct tcgcccaggg gaacaaggta accaccttca
     4261 tccccgcggg tggtatgtcc gcgaccgcct tcgggatcaa cgacaagagc atgatcgtcg
     4321 gccaatacgt cacctcgacg gcgaccccgg ggttcattcg gttgaactcc aagtcatacg
     4381 tcacgatcaa tgcgccgtcg ggaacgaacg tcgtgaacgc gcaaggcatc aataacaagg
     4441 gacaagtcgt cgggttctac ctcggcaccg acggccaggt tcatggcttc acggcaaacg
     4501 ccaacggctc gaaagctggc agcatcaccg gagtcgcaat cgcggatccg acgatccccg
     4561 tcgttcccgg agagcctggc gccactttcg tcttctccca gattttgggg atcaacgaca
     4621 agggcatcgc cgtgggctat tacggcgact ccaccaccag ccagcacggc ttcctctaca
     4681 acactcacac cggcgtgtac accttcctgg acgatcccag cgaggcgttc aataacggcg
     4741 tggaggtcac ccagatcacg ggcatcacca attcgggcga gatctcaggc ttctactccg
     4801 atgccaacgg ggtctttcat ggtttcgtcg ccaccgcgtg acgggggtgt tgtccaatgg
     4861 cgaccgggtc agagcgcgtg gcgcgctctg acccggtcgt ttcgttagcg gatcggtcga
     4921 ttttttgctg agcacacttc taacgtcgcc ccgccgccag taagttatag ttgagaacca
     4981 aggcgtcatc ggccgcaacc cacgtcccgc ggccatccag cgacgccgat ggacgcaacc
     5041 ggcgttgttt gtggaggggg cggcaatatg gcgagcggac tggaaacacg cggacaagtc
     5101 gctcggacga tggccggcac ggagggggtc gagatcaagg ccacgattcc agaaagtcag
     5161 gtggagctgg cactccagat ctatggcctg aatctggatg ataatgagcg gtacatctac
     5221 tttttcgaca cacccgagct ggaacttttt gccgagggaa tcattgcacg ggcgcggcga
     5281 attgtcgggg ccgcgcatga cagcacgatc aaatttcgcc ctgtcgaccc ggaatcggtc
     5341 ccagcgctgt ggcgcaaata cagtggtttc aagatcgagg ctgatgccag ccacaagggc
     5401 gtggtgaaat ctgcgtcgct gaccatgcca gttgccaagg gccagatcaa gcgcgtggcc
     5461 gccggcagcg cgccggtcgc cagtctgttc gccgaagagc aactgctatt tctcttgagc
     5521 atggccaaca agaaaatcga ttatacaaaa gttgttgtga tggggccctt gcgcgcctgg
     5581 cgctggaagt acatcgaccc cggtttaccc tggccgatca ccggagaact gtggcagcgc
     5641 agtgacggag cgcggttatt cgaggtgtc
//
LOCUS       DATAQL010001210         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5466221, whole genome shotgun sequence.
ACCESSION   DATAQL010001210 DATAQL010000000
VERSION     DATAQL010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5466221"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VHS97_20855"
     CDS             complement(<1..1044)
                     /locus_tag="VHS97_20855"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3450718.1"
                     /translation="MTSIGEESGIASRSPHARLNRRGLLRGLGVCVALPAFESLSLPA
                     RLAAAATQAKNLATTATGSPLRTAFIYFPNGAIPKAWWPTGEGANYELKRTLAPLEPH
                     KHTIQVLGGLNHRTAQGGPDGPGDHARGGGTFLTGVRLKKSATNIRAGVSVDQLIARQ
                     VGHLTRFPSLELATDAVHKTGACDSGYACAYQYNMSWSSPTTPMTPETNPRLVFERLF
                     GSGAPGERQANMERRRGEQRSVLDFVVTEARSMHRRLDSGDRDKLDQYLTGVRELETR
                     IEKIERMGTAVDPAVDTPPGIPPDYAEYIQMMYDLLVLAFQTDSTRVATLLLAHDGSN
                     RSFDHIGIFEGHHD"
     gene            complement(1041..>2383)
                     /locus_tag="VHS97_20860"
     CDS             complement(1041..>2383)
                     /locus_tag="VHS97_20860"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3450719.1"
                     /translation="QAITATLASPGFLFREESVATGSGTEPFPMIDEYALASRLSYFL
                     WSSMPDEELLRLAATGALRQNFSAQFARMLADPRFEEFIRHFVGQWLQARDIDSVTIN
                     AAAVLSRDEVADPEADARRARLRELSRKSGDNLTDLEKKEFDQLRLTVFGSFRRFREF
                     ELNGELRRAMRRETEMLFGHIVHNGRNVLELIDSDYTFLNERLAKYDGMTDIKGDEMR
                     RVALPPGGPRGGVLTQGTVLAVTSNPDRTSPVKRGLFILDNILGTPPAPPPPDIPPLE
                     DAAKRSSNRKPTLRETLALHRENPKCSSCHNRMDPLGLALENFNALGRWRDKERGQPI
                     DSSGKLITGEEFSTIQELKRALVSKRQRDFYRCLSEKLLTYAIGRGLEYHDVEAVDRL
                     VERLESKGGNATELLKGVVDSVPFQRGRIDEAVAANQGGGEGRREPGAELESKR"
BASE COUNT          446 a          728 c          732 g          477 t
ORIGIN      
        1 gtcgtggtgc ccttcgaaga ttccgatgtg gtcgaatgac cggttgctgc cgtcgtgagc
       61 cagcaacaag gtcgcgaccc gcgtcgagtc ggtctggaaa gccaggacca agaggtcgta
      121 catcatctgg atgtattccg catagtccgg cggtataccg ggtggcgtgt cgaccgcggg
      181 gtcaacggca gttcccattc tctcgatctt ttcaatgcgg gtttccagct cgcggacgcc
      241 ggtgagatac tggtcgagtt tgtccctatc gcccgagtca agacgccgat gcattgaccg
      301 cgcctcggtc acgacaaaat cgagaacgga ccgctgttcg ccgcggcggc gttccatgtt
      361 ggcctgccgt tcgccgggtg ccccgctgcc gaagagccgc tcaaagacca accgggggtt
      421 ggtttcgggc gtcattggcg tcgtcggcga gctccaggac atgttgtact gatacgcaca
      481 agcatagcct gaatcgcagg cgccggtttt gtggaccgca tcggtggcga gttccagcga
      541 ggggaaccgc gtcaggtgcc cgacctggcg cgcaatcaac tgatcgacgg acactccggc
      601 acggatgttc gttgcgctct ttttcagtcg aacgccggtc aggaatgtgc cgcctccccg
      661 cgcgtgatcg cccggtccgt ccggtccgcc ctgtgccgtc ctgtggttca atccccccaa
      721 aacctggatc gtatgcttgt gcggctcgag cggggccagg gtccgcttca gctcataatt
      781 ggcgccctca ccagtcggcc accaggcttt gggaatcgcg ccattgggga aatagatgaa
      841 ggccgtgcgt aaaggcgagc cggtcgcggt ggtggcgaga tttttcgcct gggtcgcggc
      901 ggcggccaat cgggccggga gggagagtga ttcgaaggcc ggcagggcca cgcatacgcc
      961 cagcccgcgc aacaagccgc gacgattcaa ccgcgcgtgc ggcgaacgtg aggcaatccc
     1021 actctcttcc ccgatcgacg tcatcgttta gactccaatt cggcccctgg ttcgcgccgg
     1081 ccttcgccgc caccttggtt tgccgctaca gcctcgtcga ttcggcctcg ctggaacgga
     1141 accgaatcga ccaccccctt taataactcg gtcgcgttac cgcctttact ttcaagacgt
     1201 tccaccaagc gatcgacagc ctcaacatca tgatactcga gaccgcgccc gattgcataa
     1261 gtgagcagtt tctcgctcag gcagcgataa aaatcccgct gccgcttgga tactagagcg
     1321 cgtttcaact cttgaatggt agagaattct tcgcccgtga tcagttttcc cgacgaatca
     1381 atcggctggc cgcgctcctt gtcgcgccag cgacccagcg cgttgaagtt ttccagggcc
     1441 aggcccagag gatccatgcg attgtggcaa gaactgcatt tcggattctc gcggtgtagc
     1501 gccagcgtct cgcgcaaggt cggcttgcga ttgctcgatc tcttggcggc atcttcgagc
     1561 ggcggaatgt cgggcggcgg gggggcaggg ggcgtcccca aaatgttatc gagaatgaac
     1621 agaccgcgct tcacaggcga ggtccggtct gggtttgagg tcaccgcgag caccgtcccc
     1681 tgggtgagga ccccgccgcg cggtccgccc gggggcaagg ccacccgccg catttcgtct
     1741 cccttgatgt ccgtcatccc gtcgtacttg gcgagccgtt cgttcaaaaa cgtgtaatcg
     1801 ctgtcgatca attcgaggac gttacgaccg ttgtgaacga tatgcccgaa aagcatctcg
     1861 gtttccctgc gcatggcgcg ccgcaactca ccattgagct cgaattcgcg gaaccggcga
     1921 aacgagccga agaccgtgag ccgcaattgg tcgaactctt tcttttcaag atcggtgagg
     1981 ttgtccccgg acttgcgact gagctcacgc aatcgcgccc ggcgggcatc ggcctcaggg
     2041 tcggctacct cgtcgcgcga gagcaccgcg gctgcattga ttgtcaccga atcgatatca
     2101 cgcgcctgga gccactggcc gacgaagtgg cggataaact cctcgaagcg cgggtcggcg
     2161 agcatccggg cgaattgagc ggagaagttt tgccggagtg cgccagtcgc agccagccgc
     2221 aataattctt cgtccggcat cgacgaccag aggaaatagg acagccgaga cgcgagtgca
     2281 tattcatcaa tcattggaaa tggctcggta ccagagccag tggcgaccga ttcctcacga
     2341 aacaggaagc cgggggacgc cagagttgcc gttattgcct ggg
//
LOCUS       DATAQL010001211         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4395639, whole genome shotgun sequence.
ACCESSION   DATAQL010001211 DATAQL010000000
VERSION     DATAQL010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395639"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VHS97_20865"
     CDS             <1..261
                     /locus_tag="VHS97_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450720.1"
                     /translation="LLNGAFCNEQAGFFANRLTRDAPDDLAKQVRRAILLTTGREPDP
                     GELKGDVAFINRLIDEEHLSARDALNQYCLLALNANAFLYLD"
     gene            275..1765
                     /locus_tag="VHS97_20870"
     CDS             275..1765
                     /locus_tag="VHS97_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450721.1"
                     /translation="MDHHSFQQPRSATAGSFCGRTRREFLWEIGGGFGSVALAGLLGG
                     DGFLARQAVAADGATPFKSPLAPKAPPLPAKAKSVIFLFMYGGPSHVDTFDYKPDLYP
                     LDGKTIAVKTFGRGGKKNEGRIVGPKWSFQQYGQSGRWVSDLFPNLATCVDDIAFIHS
                     MYAESPIHGSAMLMMNSGRLLSGNPCLGTWVTYGLGSENENLPGFVVMLDRKGGPISG
                     AKNWSSGYMPAAYQATVLRSDGVPIHDLASPTGTERKARRVLLDRLREKNEAHLASRA
                     DNSELAARIASYELAFKMQQSAPEAVDFGRESPETLALYGMDQPHTADFGRKCLIARR
                     LVERGVRFVQIYSGGAHNDDNWDAHADLVANHTHHAGNTDKPIAGLLKDLKRRGLLDE
                     TLVVWGGEFGRQPTAEFAKGTGRDHNSFGFTMWMAGGGIKGGTSVGRTDELGSNAVED
                     RFHVKNLHATILQQLGLDPNALTYFYGGLHQKLVGVEGAEPIQQII"
     gene            1806..3146
                     /locus_tag="VHS97_20875"
     CDS             1806..3146
                     /locus_tag="VHS97_20875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX3450722.1"
                     /translation="MRIQHSRRSFVRALSAAGAAIGLKADRATASADEPLAILGGQPV
                     RNPKQPFFKWPVTTEADERAWMDVLKKGKWCRLDGDYANRFEESWAQTLGAKHCQAVA
                     NGTSALITSLAALGVGPGDEVIVPPYTFVATINAVLMHHALPVFVDTDPETFQIDARK
                     IEAAITERTACILPVHLGGAAADMDTIVAVARRHNLPVVEDACQAHLAEWRNKKVGTL
                     GELGCFSFQASKNLNSGEGGAIVTNSDDLFEKSKSFQNNGRAAANAGFGYARNGANLR
                     MTEFQAALLSAQLTRLEEQSRRREQNAAYLTRQLQEVPGIKPAKTYDGCTRNAYHLYM
                     FRYDPQPFDRVPRARFLQALHAEGIPCSGGYQPLYKEPFLANTLGSRPFQAVYAPKRI
                     SDYLEQIQCPANDRLCETAVWLTQTTLLGTRTDMDQIAEAVRKVQKQAAKLLPA"
     gene            complement(3154..4098)
                     /locus_tag="VHS97_20880"
     CDS             complement(3154..4098)
                     /locus_tag="VHS97_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3450723.1"
                     /translation="MTRPLTQSDPGPAGSNRRGMLRQTAGAAVLGVTALGGKLPAQDS
                     PSAERVVKNGRINQSIVYWCFEKYWDMDQAAKVARQLGCTSIELIASKFFPVLKQHGL
                     KCAIGTVDMSPDPPFVKGFNNPKYREQVQKATREAIDACADFGSKNVICFTGMREGIP
                     DDVGADNCVEGFKQIIGHAEKKGVTLCLEMLNSRVSSHPMKGHPGYQGDHTDYCIDII
                     KRVGSPNLKLLFDIYHVQIMDGDIISRLRQHRDYIAHVHTAGNPGRGELDQKQEINYP
                     PIMETLLEIGYHGHVGQEFIPTRDPLEGLRQAVALCDV"
     gene            4409..>4639
                     /locus_tag="VHS97_20885"
     CDS             4409..>4639
                     /locus_tag="VHS97_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450724.1"
                     /translation="MENSIHEVQVEDIEVGRPSTMSATAERRWVTATLLVAMMVTAIE
                     QLVVSPAMPTIIAQLKGFEIYPWVISAFLLAAT"
BASE COUNT          900 a         1357 c         1472 g          910 t
ORIGIN      
        1 ctcttgaacg gtgcattttg caacgaacaa gccggctttt ttgccaatcg tctgacgcgc
       61 gatgccccgg acgacctggc aaagcaggtt cgccgcgcga tccttctgac cactggccgt
      121 gagcccgacc cgggcgaatt aaagggtgac gtggctttca tcaatcgctt gatcgacgaa
      181 gaacacttga gcgcgcgcga cgcgctcaat cagtattgcc tgctcgcctt gaatgccaat
      241 gcgtttttgt atctcgattg aggctcgatt cgacatggat catcactcgt ttcaacaacc
      301 tcgtagtgcc accgccggct cgttttgcgg caggacgcgt cgcgagtttc tctgggagat
      361 cggcggtgga ttcgggtcgg tcgcgctggc tgggttgttg ggcggcgacg gttttttggc
      421 tcggcaggcc gtcgcggccg atggtgcgac gccattcaag agcccgctcg cgcccaaggc
      481 gcctccgttg ccggcgaagg ccaagagcgt gatctttctc ttcatgtacg gcggcccgag
      541 ccatgtcgat accttcgatt acaagcccga tctgtatcca ctcgatggca agacgatcgc
      601 cgtcaagaca ttcggccggg gcggcaagaa aaacgaaggg cgcatcgtcg ggccgaagtg
      661 gtcgtttcag cagtacggcc agagcggcag gtgggtttcg gacctgtttc cgaatctggc
      721 aacgtgcgtc gacgacatcg ccttcatcca ctcgatgtat gccgagtcgc cgatccacgg
      781 ctcggccatg ctgatgatga attctgggcg gctgctctcg ggaaatcctt gcctgggcac
      841 gtgggtcact tacggcctgg ggagcgaaaa cgaaaatctg ccgggctttg tggtgatgct
      901 ggatcgcaag ggggggccga tcagcggggc caagaactgg tcgagcggct atatgccggc
      961 cgcgtaccag gcgacggtgc ttcgttccga cggcgtcccg atccacgacc tggcctcgcc
     1021 gacgggtaca gaacgcaagg cgcgccgggt cttgctcgac cggctgcgtg aaaaaaacga
     1081 ggcccacctg gcgagccgcg ccgacaattc cgagctggcc gcgcgaatcg ccagctacga
     1141 gctcgctttc aagatgcagc agtcggctcc cgaggcggtc gattttggcc gcgagtcccc
     1201 cgagaccctt gctctctacg gcatggacca gccgcacacg gccgactttg gccgcaagtg
     1261 cctgatcgcg cggcgcctgg tggagcgagg agtccggttc gtgcagatct attccggcgg
     1321 cgctcataat gacgacaact gggacgccca cgctgacttg gtcgccaacc acacccacca
     1381 cgcgggcaac accgacaagc cgatcgccgg gctgctcaag gacctgaagc gcaggggttt
     1441 gctcgacgaa acgctggtgg tctggggggg cgagtttggc cgccagccca ccgccgagtt
     1501 cgccaagggg acggggcgcg accacaactc atttgggttt acgatgtgga tggccggcgg
     1561 cggaatcaag ggaggcacca gcgtcggcag gaccgatgag ctggggagca acgccgtcga
     1621 ggacaggttt cacgtcaaga acttgcatgc tacgatcctg cagcagctcg ggctcgaccc
     1681 caatgcgctc acctactttt acggagggct acaccaaaag ctggtggggg tagaaggggc
     1741 agagccgatc cagcagatca tctgatgtcc ggtcgagtcg catccgtttg aatcgagatt
     1801 gactcatgcg catccagcat tcacgtcgca gtttcgtgag agcgctctcg gcggcggggg
     1861 cagccattgg acttaaggcc gaccgcgcga cggccagcgc cgacgagccg ctggccatcc
     1921 tggggggtca gccggttcgc aacccgaagc aaccgttctt caaatggccg gtgaccacag
     1981 aggcggacga gcgcgcctgg atggacgtgc tgaaaaaggg gaagtggtgc cggctcgacg
     2041 gcgactacgc caatcgtttc gaggaatcgt gggcccaaac gctgggagcc aagcattgcc
     2101 aggctgttgc caatggcaca agcgcgctca tcacgtcgct ggcagcgctc ggggtcggtc
     2161 cgggcgatga ggtgatcgtc ccaccgtaca cgttcgtggc cacgatcaac gccgtcttga
     2221 tgcaccatgc actgccggtt tttgtcgata ccgacccgga gacgtttcag atcgacgcgc
     2281 gcaagattga ggcggcgatc acggaacgga ccgcctgcat cttaccggtg cacctgggtg
     2341 gtgcagcggc cgatatggac acgatcgtgg ccgttgcgcg caggcacaac ctgccggtcg
     2401 tcgaggacgc gtgtcaggcc catctggccg aatggcggaa caagaaggtt ggaaccttgg
     2461 gtgagctcgg ctgcttcagc tttcaggcca gcaaaaacct gaactctggt gaagggggcg
     2521 cgatcgtcac caatagcgac gacctctttg agaagtccaa gagttttcaa aataacggcc
     2581 gagccgcggc caacgctggc tttggctacg cccgcaacgg ggccaacctg cggatgaccg
     2641 agtttcaggc cgctctgtta tccgcacagc ttacacggct cgaggaacag tcgcgccgcc
     2701 gcgaacaaaa cgcagcctat ctcacacggc agttacagga ggtccccggg atcaagccag
     2761 cgaaaaccta cgatggctgc acgcgcaatg cctatcatct ctacatgttt cggtatgatc
     2821 cccagccgtt cgaccgggtt ccccgggccc ggttcttgca ggcgctgcac gcggagggga
     2881 tcccgtgctc aggcggctat caaccgcttt acaaggagcc gtttctggcc aacacgctcg
     2941 ggtcgcggcc gtttcaggcg gtctatgcac ccaagcggat ctccgattac cttgagcaaa
     3001 ttcaatgtcc ggccaacgat cgcctctgcg agacggccgt ttggctgacg cagacgacgc
     3061 tcctgggcac tcgcactgac atggaccaga ttgccgaggc ggtgcggaaa gtccagaaac
     3121 aggcggcgaa gctcttgccg gcgtgaggat ccgtcagacg tcgcaaagcg caacggcctg
     3181 ccgcaggccc tccaaggggt cgcgggtggg gatgaattcc tggccgacgt gtccgtgata
     3241 accgatttcg agcagcgtct ccatgattgg cggatagttg atttcttgct tctgatcaag
     3301 ctcaccgcgg cctgggttac cggctgtgtg cacatgcgcg atgtagtcac gatgctggcg
     3361 caggcgcgaa atgatatcgc cgtccatgat ctgaacgtga tagatgtcga agagcagctt
     3421 gagattgggt gagccgaccc gcttgatgat atcgatgcag taatcggtat ggtccccttg
     3481 gtaccccgga tggcccttca tcgggtgaga gctcacgcgt gaattgagca tctccaagca
     3541 gagagtcacg cctttttttt cagcgtggcc gatgatctgt ttgaagccct cgacacagtt
     3601 gtcggcgccg acgtcgtcgg ggatgccttc gcgcatgccc gtgaagcaga tcacgttttt
     3661 cgatccaaag tcggcacatg cgtcgatggc ctcgcgcgtg gccttctgca cttgttcgcg
     3721 atatttgggg ttattgaatc ccttgacaaa gggagggtcg ggactcatgt cgacggtgcc
     3781 gatggcacat ttgagtccgt gctgcttgag gaccgggaaa aacttggagg cgatcagctc
     3841 gatgctggtg cagcccagtt gcctggcgac cttggccgcc tggtccatgt cccagtattt
     3901 ctcgaagcac cagtagacga tggattgatt gatccgaccg tttttcacga cacgctccgc
     3961 ggatggagaa tcctgggccg gtagctttcc ccccagggct gtcaccccca atacggccgc
     4021 gcccgcggtc tgcctgagca tgcctcgacg gttgctacct gcaggccctg gatcagattg
     4081 ggtcaagggg cgagtcatcg cgcgagtctc cgatttgcgg gtgatggcga cctacgacag
     4141 tgatccgttg gtacacgata cgcgacttca ggccattttt cgagacgcga cccagcaccg
     4201 cgagcccgat cgatcccgtt cgtgtctccc ttggcaaacg cggtacaatg tcacgttcca
     4261 tgcgcgcgcg gcgcccccaa ggggcagaaa gcggccgacg ccatgaactc tttgggctgg
     4321 atgctcttag cacgacgaca gttacggggc aggaatgccg tattgggaga gtgcctcttc
     4381 aagtgaaatc gagaggaacc ggggggaaat ggagaattcg attcatgaag tccaagtgga
     4441 agacattgag gtgggccgac cgagtacgat gtcggcgacg gcggagaggc gatgggtaac
     4501 ggcgacgctc cttgtggcca tgatggtcac tgccatcgag caattggtcg tctcgccggc
     4561 catgccgacg atcatcgccc agctcaaggg gtttgagatt tatccctggg tgatctcggc
     4621 atttttgctg gcggcgaca
//
LOCUS       DATAQL010001212         4975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4702192, whole genome shotgun sequence.
ACCESSION   DATAQL010001212 DATAQL010000000
VERSION     DATAQL010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4702192"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..1671)
                     /locus_tag="VHS97_20890"
     CDS             complement(391..1671)
                     /locus_tag="VHS97_20890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX3450725.1"
                     /translation="MHRVVVTGIGMVAPNGIGRREFSEAIFEGRSGVDYIESFDTSGL
                     TIKIAGEVKNFDVLPYLGEHKKNLKLMSRAVRFAVGAAAMAVEDAGLETARLDPARFG
                     VCMGAGITPVDVGELVNPILRSVGSDGTLDMGRFTLARSESIFPLWLLQHLPNMAAAH
                     ISILQHAMGPNNTIVTACAAGTQAVGEAFRLIARGDADVMLAGGCDSRLDPQLFVAYS
                     AMKAVSASVRPPTEVSRPFDADRDGFVLGEGSAMLVLESYRRARRRGATIYAEITGYG
                     SSFDAFGITRPEPEGKGAALAMMAALREAHIDPSEVDYINAHGTSTKLNDLMETVAVK
                     CVFGHRAASIPMSSQKSMIGHLIGASGAVEAAATALSLHRGVVPPTINLATPDPDCDL
                     DYVPNTSREMPLETAVSNSFGFGGQNASLVMTRT"
     gene            2199..3392
                     /locus_tag="VHS97_20895"
     CDS             2199..3392
                     /locus_tag="VHS97_20895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3450726.1"
                     /translation="MPLSLAARAGLIAPSATLAMGAEAKRLKSKGIEVLDFALGEPDF
                     ATPSNIQEAAFKAIRTGQTHYTPPAGIPELRQALADHYTHDKNLMTEPGQVIISNGAK
                     QSIHNALMAVCGPDDEVIIPAPYWVSYADLVKLTGATPVVITTTEEAEFKLTPSQLLA
                     AITPRTKLLMINSPSNPTGVVYERSELAALAHALLQTDVGVLSDEIYEQLTYGGTLPT
                     CFAALLPGLADRTITISGVSKTYAMTGWRIGWAIAPLPVAKFMGDLQSQETSNPCSVS
                     QLAALEAITGPQDSVAAMKTQFQQRRDFVLKRIEELPGVTCLPPGGAFYAFMNISGHL
                     GRTLGGKKIVDSTAFCMTALSEARVALVMGSAFGAEGYARLSFATDLKTLGQGFDALA
                     QFLHG"
     gene            3601..4782
                     /locus_tag="VHS97_20900"
     CDS             3601..4782
                     /locus_tag="VHS97_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450727.1"
                     /translation="MKIAPCILLAIAAMLSASGCRAWNTGPLSLVPAPRPLGERTLDK
                     DAFIAEHNKNAELIQSLEAKPTIGVASRILKAGADGRLALERPRNFKLELDVVGATKK
                     ADIGSNDEEFWFWVANDKDKSVYWCNYDELNSSALAITYQPEWIIESLGLKPISAEEA
                     AKIKVRDGEVRGTSELVFPAIRNGNETYTRMMIVRNQTRRIEQHRIYAGNHQSSSSGS
                     RSLIAQAVVSRFAEYDVGSADAAGGQTCYLPEHVKLEWKRDQLTLDVVMKDVKVNQFD
                     SSRSTALFVEPVLPGHERRNLAELNRMDRKDSRTTVRQTLPPPEPGNGVKLGRPTPIQ
                     DDTSMVPRVPASAAARSRGATTSPLEALVTPPLPVSPDNAMAQPASGPWAQANLFQIE
                     R"
     gene            complement(4812..>4975)
                     /locus_tag="VHS97_20905"
     CDS             complement(4812..>4975)
                     /locus_tag="VHS97_20905"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX3450728.1"
                     /translation="DREAEVIRLYHLKFLNYRQIGKQLGLPENSIGPILAKARTKLRQ
                     AAEKQQSAS"
BASE COUNT         1054 a         1446 c         1460 g         1015 t
ORIGIN      
        1 cagactcgag ccaccacccc agagaggcgt cctggtagac gacaggatgg cggcaaaccc
       61 ggcggcggtc gccggctcca ggacgcggct tactcacgac tctcaccttg aaattcgagg
      121 gattcattct gtgaacggcg ccgcgcacgc atcattccca tacttggcag attgcaatcc
      181 gctcgctaac tggcccatcc atgcgcggaa gcgctgacga tcgccttcga taagaaatcc
      241 tagttcgtgg tcgggccgct gtcaaaaaga caaataagtc tgtagtcatt aacttattgg
      301 caactagtga ataggtcggc cggtaaactc ccgaagtgag acgcctgatc gctgtggggc
      361 ctttgaccgg tgttttggac agcttcaaaa tcacgttcgg gtcatgacga gcgaagcgtt
      421 ctggccgccg aagccaaagc tattggacac agcggtttca agcggcatct cccgtgaagt
      481 gttgggcaca tagtcgagat cacagtcagg gtcgggcgtt gcgaggttga tggtcggagg
      541 gacaacgccg cgatgcaatg aaagcgcagt cgcggccgct tcgacggcgc cggaggcgcc
      601 aatcaggtgg ccgatcatcg acttctgact gctcatggga atcgaagcag cgcggtggcc
      661 gaagacgcac ttgacggcga ctgtctccat gaggtcgttg agcttggtgc tggtgccgtg
      721 ggcattgatg tagtcgacct cggaagggtc gatgtgagct tcgcgcaggg ccgccatcat
      781 cgcgagagcc gctcccttgc cctcgggttc ggggcgagtg attccgaaag cgtcaaatga
      841 ggatccatag ccggtgatct cggcataaat cgtggcaccg cggcgtcgag cacggcggta
      901 gctttcgagc acgagcatcg ccgatccttc gcccaggacg aagccatcac gatcggcgtc
      961 aaacggccgg gaaacctcgg taggcgggcg gaccgacgca ctgactgctt tcatcgccga
     1021 ataggccacg aagagttgcg gatcgagccg gctgtcgcaa ccgccggcga gcatcacgtc
     1081 ggcatcgccc cgagcgatca aacgaaatgc ctcgccgact gcctgcgtcc cggcggcaca
     1141 cgctgtcacg atcgtgttgt ttgggcccat ggcgtgctgc aagatcgaaa tatgcgcggc
     1201 cgccatgttg ggtaagtgct gcagcaacca gaggggaaaa atcgactcgg accgtgccaa
     1261 tgtgaaccgg cccatatcca gcgtgccatc agaaccgacg cttcttaaga tcggattcac
     1321 caattccccg acatccaccg gcgttatgcc tgctcccatg cagaccccga accgcgcggg
     1381 gtcgaggcgg gcggtctcaa ggccggcgtc ctcaacggcc attgcggccg ctcccacggc
     1441 gaatcgcacc gcccggctca tcagtttgag gtttttcttg tgttctccca gatacggaag
     1501 aacatcgaag ttcttgacct cgccggcaat cttgatggtc agcccagacg tgtcaaaact
     1561 ttcaatgtaa tccacgcccg aacgcccttc gaaaatggcc tcgctaaatt cgcgtcgacc
     1621 aatcccgtta ggtgcgacca taccaattcc ggtcacgacc acgcggtgca ttttgtcccc
     1681 cacggtcatt ctgtcgcaat ggctcaagtg atgtggtgtg ggtgattcac tcttgggcgc
     1741 tgtccttcct tgctcggtcg agttaaactg aatcgcctgg cgcccgcagt tatcggaccg
     1801 tccccgggcg aaactggaac atccttctgc cttaaaccta gaatgttccg gggctgacga
     1861 gatctaagta atctctgtct attaacgaat ttggcgcctt ctcaggtgct cgacaaagcc
     1921 gccgatatat ggagaaccgc gataatcccg gcttccataa tcgacacatc ttggaccgtc
     1981 tatcgacgga ccagccggtc atccctaaag attgcccggc cagcataatt taccatgcgt
     2041 cgccggattc agcaaaccgt ttgagagata tgggcaaatt gcgtggatgt ggcaacttta
     2101 gttttgtatc gggttttcgt gaaccgccgc cagttcgctg gggtcaaaaa tctgagtgca
     2161 agtaggacgg gttcgtcttc gagcggagtg ataatgccat gccgctaagt ctggctgcgc
     2221 gtgcgggatt gattgcgcct tcggctacgc tggcgatggg tgccgaggcc aagcgattga
     2281 aatcgaaagg cattgaggta cttgactttg cgctcggcga gcccgatttt gcgacaccga
     2341 gcaatattca ggaagccgcg ttcaaggcga tccgtaccgg tcaaacgcat tatacccctc
     2401 ctgccggcat tcccgagctc cggcaggcgc tcgccgacca ctatacacat gataagaact
     2461 taatgaccga gcccggccag gtaatcattt caaacggggc gaagcagtcg attcataacg
     2521 cgctcatggc cgtatgcggg cccgatgacg aggtcatcat cccagcgcca tactgggtaa
     2581 gttatgccga cctcgtgaag ctcacaggcg ccacccccgt cgtcatcacg actacggagg
     2641 aggcagagtt caaactgacg ccctctcaac tcctggcggc gatcacgcct cgcaccaagc
     2701 tgctcatgat caacagtccg tctaacccga cgggtgtggt gtacgagcga tccgagctgg
     2761 cggcacttgc ccacgcgctg cttcaaaccg atgtcggggt tttgtccgac gaaatctacg
     2821 aacagctgac gtatggtggc acgctgccga cgtgcttcgc cgccctcttg ccggggcttg
     2881 cggaccgaac catcacgatt tcgggggtaa gtaagactta tgcgatgacg gggtggcgga
     2941 tcggctgggc catcgcacct ctgccagtgg caaagtttat gggggacctg cagagccagg
     3001 aaacgagcaa cccctgctct gtgagtcaat tggccgccct cgaagcgatt actgggccgc
     3061 aggattctgt tgcggcgatg aaaacccagt ttcagcaacg gcgtgatttc gttttgaagc
     3121 gtatcgagga gctgccgggt gtgacttgcc tgccgcccgg cggggcattt tatgcgttca
     3181 tgaatatctc ggggcacctg ggtcgaacgc tcggcggcaa aaaaatcgtt gattcgaccg
     3241 ccttttgcat gactgcactt agtgaggccc gcgtcgcgct cgtgatgggc tcggcgtttg
     3301 gcgccgaggg ttatgcacga ctttcgtttg cgactgacct gaagacgctt ggccagggct
     3361 tcgatgcgct cgctcagttc ctgcatggtt gagagcagac tgaaaactag cctgagaaac
     3421 ggtgtaatca gcccggattc atcgattcgg gaatgtgccg caaatcgccc ttgtcccaaa
     3481 tggcgtcttg agataagtta cgtaccctct cgaaatacca tctcttgggt tcggttcatc
     3541 gttcataatg tcattaggcg cggcgattcc gcgcgacgat ccgactcagg ggagaaagcc
     3601 atgaagatcg cgccctgcat cttgctcgcg atcgcggcga tgctgagcgc cagtggctgc
     3661 cgcgcctgga acacgggccc tctctcgctg gtccccgcgc cccggccgct cggagaacgg
     3721 acgctggata aggacgcctt tatcgccgag cacaacaaga atgccgaact gattcagagc
     3781 cttgaggcca agccgacgat cggggtcgct agcaggatct tgaaggctgg cgccgacggg
     3841 cggttagcac tcgaacgacc ccgcaacttc aagctagaac tcgacgtcgt gggggcaacg
     3901 aagaaggccg acatcggatc gaatgacgag gaattctggt tctgggtggc caacgacaaa
     3961 gacaagtcgg tctattggtg caattacgac gagctcaatt cgagtgcgct ggccatcacc
     4021 tatcagccgg aatggatcat cgaatcgctc ggcttgaaac cgatttctgc cgaagaggcg
     4081 gccaagatca aggtacgcga cggtgaagtg cgtggaacgt cggagctggt atttccggcg
     4141 atcaggaacg gcaatgaaac ttacacccgg atgatgatcg tccgaaacca aacccggcga
     4201 atcgaacagc atcggatcta tgccggcaat caccaatcgt cgtcgtcggg ctcgcgaagc
     4261 ctgattgctc aggccgtcgt cagccgcttc gccgagtacg acgtcggctc cgccgacgcg
     4321 gcgggcgggc agacgtgcta cctaccggag cacgtcaagc tcgaatggaa acgtgatcag
     4381 ctgacccttg atgtggtcat gaaagacgtc aaagtcaatc agtttgattc atctcgcagc
     4441 accgctctct ttgtcgaacc ggttctcccc ggccatgaac ggagaaactt ggcggaactc
     4501 aatcgaatgg atcggaagga ttcgcggact acggtgcgac agactttgcc tccgcctgaa
     4561 cccgggaacg gtgtgaaact cgggcggccg acaccgatcc aggatgacac atcgatggtg
     4621 ccccgcgttc ccgccagtgc ggccgcgcgg tcgcgtggcg cgacaacttc gcccttggag
     4681 gcacttgtga ccccgccgtt gcccgtctcg cccgacaacg ctatggcgca gcccgcaagc
     4741 ggcccatggg cccaagccaa cctgtttcaa atcgaacgct gataagcagt tgtgtcgttg
     4801 ggacacattg atcaacttgc gctttgctgc ttctcagccg cctgtcgaag tttggtgcgg
     4861 gccttcgcca ggatcggacc gatcgaattc tcaggcaacc cgagctgctt gccgatctgg
     4921 cgatagttca aaaacttgag gtgataaagc cgaatgacct cggcttcgcg gtcgc
//
LOCUS       DATAQL010001213         3236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4245346, whole genome shotgun sequence.
ACCESSION   DATAQL010001213 DATAQL010000000
VERSION     DATAQL010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4245346"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VHS97_20910"
     CDS             complement(<1..313)
                     /locus_tag="VHS97_20910"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3450729.1"
                     /translation="MLQNAGATAVADAVVKDTTTQTFVKDVIEESKRQPVLVDFWAEW
                     CGPCKQLTPVLEKVVRTAKGKVKLVKMDIDKHPAIPGQLGIQSIPAVFAFVNGQPVDG
                     FM"
     gene            complement(607..1074)
                     /locus_tag="VHS97_20915"
     CDS             complement(607..1074)
                     /locus_tag="VHS97_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450730.1"
                     /translation="MALAATSGAFAAGGYIQASSEEDQIAKDTKPTKITMNSTDAAKG
                     LKNDKGTIILNESGTYFDIVGVQVGSRGGMGLVRLFFKTNGKDVDNSNCEQMVPTADF
                     TTVMISQGVGEYKKGDKVEAWMSGSAPGIGLVYKKPTGEPAVPSVIFSMWKID"
     gene            complement(1558..2064)
                     /locus_tag="VHS97_20920"
     CDS             complement(1558..2064)
                     /locus_tag="VHS97_20920"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEX3450731.1"
                     /translation="MPATPDDLFAYLDRLGIAHKTVAHAPVFTVEEAQTLRGQVAGAH
                     TKNLFLKDKKDALFLVVALEEAAIELKSLHRRLGASGRFSFGSAELLRATLGVEPGSV
                     TPFAALNDTDARVRVVLDAAMMGHDLLNYHPLRNTMTTSIARDDLVRFLTATGHPPRI
                     VAVADALA"
     gene            2441..2515
                     /locus_tag="VHS97_20925"
     tRNA            2441..2515
                     /locus_tag="VHS97_20925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2473..2475,aa:Gly,seq:gcc)
     gene            2845..>3236
                     /locus_tag="VHS97_20930"
     CDS             2845..>3236
                     /locus_tag="VHS97_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450732.1"
                     /translation="MPMLQSLRKVSGTSSPRTLKKPGVKTSASSAPATLPSQSLDRPI
                     FWIVAGPNGSGKSTAYEDADIEAFARSVWIINPDALTRRIQRVEGQNLAEANLEAVKR
                     IEIWLDASIQAHQTVGVETVLSTDKYRRL"
BASE COUNT          616 a          985 c          998 g          637 t
ORIGIN      
        1 ccatgaagcc gtcgaccggc tggccgttca cgaaggcgaa caccgccgga attgactgga
       61 tgcccagctg ccccgggatc gccgggtgct tgtcgatgtc catcttgacc agcttgacct
      121 tgcccttggc ggtccgcacc accttttcca gcacgggagt cagctgcttg cagggcccgc
      181 accattcggc ccagaagtcc accagcaccg gctggcgctt ggactcctcg atcacgtcct
      241 tcacgaaagt ctgggtggtc gtgtccttga cgaccgcatc cgcgaccgct gtcgccccgg
      301 cattctgcag catctcgatg gtccttatga aattcacggg tcctttgtgg attcccaacg
      361 gctcggcgca actggcggat ttgcccggct ccggtggcct ccgaaacctc gagccagcca
      421 attaaatggt gtccatggaa ttgattgcaa tcggggtcaa gagccggcgg catgagccgt
      481 gccgctgcgt accctcccgc ctcgggggag ggagaatgat caccccaccc cacccgcacg
      541 gatcgtccta aaagcaaagg ggcgccatga gggcgccccg atggaaacac gtcgatcagc
      601 ctgaaatcag tcgatcttcc acatcgagaa aatcacgctt ggcaccgccg gctcgccggt
      661 cggcttcttg tagacgaggc cgatgcccgg cgcggagccc gacatccagg cctcgacctt
      721 gtcgcccttc ttgtattcgc cgacgccctg cgagatcatg acggtggtga agtcggcggt
      781 cgggaccatc tgctcgcaat tgctgttgtc gacgtccttg ccgttggtct tgaaaaagag
      841 ccggaccagg cccatgccgc cgcggctgcc gacctggacc ccgacgatgt cgaagtaggt
      901 cccgctttca ttcaggatga tggtgccctt gtcgttcttg aggcccttgg cggcgtcggt
      961 cgagttcatc gtgatcttgg tgggcttggt atccttcgca atctggtctt cctcgctcga
     1021 cgcctggata taaccgccgg ccgcgaaagc gccgctggtc gccgccagag ccatggccga
     1081 cacgaagatc gcactgagga tagttctcat cactgcctcc cattgggggt tggactacga
     1141 gataaagagt tccgaagagc gatgggtgag cctcttcgac ctgtggctta aggcaagtta
     1201 gtgggctgtc ccttcgaacg gcttgcatga actccaccct ggcatttgca atggacatgc
     1261 cacctccgcg agtcccgcgg gccggcggcg cccaaagccg tcaatgacgc tgaatcaagc
     1321 tcctaggggt agaatccctc ccgattcggc cgaaaatgac ttaaaaagct cgccctcgcc
     1381 acgcgctccc tttatctgcg gctgctcaat atgtaatcag gctcgcctgg cgtgcggtcg
     1441 tctgctcaaa aaatatgcat aaaagtgagt tttcacttgg ctctacgatt ggggcgaggc
     1501 tcccgctggc gggcccgaaa cccttgcagt tccctccacg accggagggc gattgggtca
     1561 ggccagcgcg tccgcgaccg ccacgatgcg gggcgggtgg ccggtcgcgg tcaggaagcg
     1621 gaccaagtcg tcgcgcgcga tcgaggtggt catggtgttg cgcaacggat ggtagttgag
     1681 caggtcgtgc cccatcatgg cggcatcgag caccacgcgc acccgagcgt ccgtgtcgtt
     1741 cagcgccgcg aacggggtga cggagccggg ctcgaccccc agcgtcgcgc gcagcagctc
     1801 ggccgagccg aacgagaacc ggccgctcgc ccccagccgc cggtgcagcg acttgagctc
     1861 gatcgccgcc tcctccagcg ccacgaccag gaacagggca tccttcttat ccttgaggaa
     1921 caggttcttg gtatgggcgc cggcgacctg gccgcgcagc gtttgcgcct cctcgaccgt
     1981 gaacaccggc gcatgggcca cggtcttgtg cgcaatgccg agccggtcga gataggcgaa
     2041 caggtcgtca ggcgtggcgg gcatgctctt ttggaggggc tggcgagatg ctttacttgg
     2101 ctggcgacac acccaaagcg tcgttcaggt ggtgcgtcag tgcgcatccc ttttgtcgcg
     2161 gaagagggtg aagggacgct aggcacgtag ggcgtttcac tccctcgccc catccgaaac
     2221 tcgggtttac ccgagtttcg gcaccttcaa gtgccaaagt cggatatatc cgattttgga
     2281 tggagagggt ggggtgaggg gggccacgat gtttccgtac cgcactttcc ctgcccctcg
     2341 aaccttcgct gcgcgctccc tccgccgcct tggttgcaaa tccatccgcc atttggcata
     2401 gcatatcgga ggcggtccag accgccttgc ccaaacggat gcgggtgtag ctcaggggta
     2461 gagcacgacc ttgccaaggt cggggtcgag ggttcgaatc ccttcgcccg ctccaaaaat
     2521 tcagtgcctg ttggcgtcgt gatgtgggat gggtgcgggg ccgagcgggc cgggggctcg
     2581 tgcgttcgcc gtcgccagct cgatacgctt ccaaatacga acgtgggcac cgccaaaaat
     2641 ggaaggccgg cgcggcacgc ttttgggcca attccagaat cggtaaacta acgctacata
     2701 ggtagcggca cgaggatgag agcatggcca gggtagtatt taaaaagctt aaggctggtc
     2761 gaagttccgg gcctgccgcg cagtccgtgg gcaggaagcg cgtcttgaag acggaaggtg
     2821 ggtggaagac cgttcgaact cttgatgcca atgctgcaat ctttgaggaa ggtctccggt
     2881 acgtcttcac caagaacgtt gaaaaagcca ggcgtgaaaa caagcgcatc gtcggcgcca
     2941 gcgaccttgc catcccaaag ccttgaccgt ccgatctttt ggatcgttgc cggaccgaat
     3001 gggtccggca agagcacggc gtacgaagac gcggatatcg aagccttcgc gcgttccgtt
     3061 tggatcatca acccagatgc actaactaga cgcattcaac gagtggaggg ccagaatctt
     3121 gcagaggcaa atctcgaagc agtgaagcgc atcgagattt ggctggacgc gtcgattcaa
     3181 gcccaccaga cagtcggagt ggagactgtt ctttcaaccg acaagtatcg ccgcct
//
LOCUS       DATAQL010001214        12227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4092899, whole genome shotgun sequence.
ACCESSION   DATAQL010001214 DATAQL010000000
VERSION     DATAQL010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12227
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4092899"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VHS97_20935"
     CDS             complement(<1..724)
                     /locus_tag="VHS97_20935"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX3450733.1"
                     /translation="MPLPLKVQKSKLVNSHDESVRLRGVNAASLEWSSDGEGHILDTV
                     KTAIKDWRVNVIRLPLAQDRWFGKAPEQKDEGKAYRELVNKIVDFCAQQGCYIMLDLH
                     WSDAGEWGKQIGQHVMPDQNSLEFWKSCASNYKNHPAVIFDLYNEPHDVSWDVWLNGG
                     QVEEKAYRGNPAKSFQAVGMKALLEAVRATGAKNLIVAGGLDWAYDMTGFLTGKQLAD
                     PTGNGVIFANHAYPFKGDTVERW"
     gene            1198..2610
                     /locus_tag="VHS97_20940"
     CDS             1198..2610
                     /locus_tag="VHS97_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450734.1"
                     /translation="MHDPRHNSRRSFLKFAAAASAASTLATTALAADGVQELRPTVEP
                     PKPAGPNDRIQIATIGMGIIGFIDTACALKVPGVELVAAADLYDGRRMHAKEEFGDKI
                     QTYVDYREILGRKDVDAVLICVPDHWHARMSIDAMKAGKAVYCEKPMIRGVEEGPGVI
                     AVQKETNAVFQVGSQFASSVLYDKVKDLIRTGAIGKLHAIEARYNRNSDTGAWQYSIP
                     TDASTETIDWDRFLGDAPKRPFDPIRFFRWRNYWDYGTAVAGDLFIHLLTGIHHATGA
                     IGPTRVDAMGGRRYWEDGREVYDVIMGLLDYPETDTHGGFTLSLCTDFEDGGGPGTAF
                     RFVGTDGVIDVSFKELTLTLMGIEHSTPDQILNGYNSVKTFSIAQQKAFAAKLLAEHP
                     SSSTKRAPKQTEKFVVPKGYDERFDHMVNFFTSIREHKPVYEDATFGLRAAAPALLCN
                     ESYRRKREIGWDPVEMKIVG"
     gene            2729..4423
                     /locus_tag="VHS97_20945"
     CDS             2729..4423
                     /locus_tag="VHS97_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3450735.1"
                     /translation="MGSPGNTPAFASNRGNGPYAPLRSLPPLPEDWRSLPRALVIRAR
                     ARWSGEAMADSTGASLTYGQTLIRALVLGRVLARTWGIAEHVGILVPPTVPAAVANLA
                     VSLWGKVPVNLNYSASQSLVDASVDQCGITHVLTSAKVLDRFKITPNAKLILLEDIPK
                     QVRLADKLWAAAAAKLVPLGALGALVPGLRGDHLDATATVIFTSGSTGDPKGVVLSHR
                     NILSNAHQIGEQVCMKPEEVLLGVLPFFHSFGFTITIWTAICLNKKVVYHFNPLDSRT
                     IGKLCEQHRVTLLTGTPSFTRMYLKSCPASQFKTITHWIVGAEKLKPELARDLTATLN
                     IEPLEGYGCTELSPVVAVNVPREVELPGGRKIYGNRLGTVGLPLPGTAIKTVNPDTGA
                     DLPVGAEGMIAVKGPQVMVGYLSRPEATAQVLKDGWYGTGDLGYLDPDGFLKITDRIS
                     RFSKIAGEMVPHIKIESAIIELTGVDENHVAVTGVPDPKHGERLCVLYTDMGISPTEV
                     QHRLNAGPMPKVWIPSPSDFVQVEAIPITSTGKVDLRRLKEIALTKCAGEEISRRA"
     gene            4603..5928
                     /locus_tag="VHS97_20950"
     CDS             4603..5928
                     /locus_tag="VHS97_20950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEX3450736.1"
                     /translation="MGIVLRVTSGPHTGQEFLVDHCATFTVGRSSRVQFAMVDDLALS
                     REHFQVDNDPPLCHLIDLGSTNGTKVNGLRVGRALLRDGDEITAGDSTFLLRFVGDSQ
                     DTLKFATCAACGARVPLGLIATDPLGEVLSDAKGPDVWLCSNCRASRLKFPRTHPDYL
                     IEEWIGGGGMGEVFRARQLSRRRPVAIKMISANNALQEKASSYFRREIEVLRDLLMRG
                     GQSHPSIVAFYEIYEIDSQFQLVMEYVDGMNALDWIKGLERPLPTSSGAQIGRHLLSA
                     LDYAHSKGYVHRDVKPTNLLVMGPIHRPRVKLTDFGLAKSFAENGGFTKLTRQGDIGG
                     SVGFISPDHIREFSDIREPADIYCAAATLFFLLTNKYPYLGFDPRERGSYEMILQHPP
                     VPLRAFRPDAPEGLEKVLLKALQKQPRDRWKSAQAMGEALRPFASPSSS"
     gene            6183..7073
                     /locus_tag="VHS97_20955"
     CDS             6183..7073
                     /locus_tag="VHS97_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEX3450737.1"
                     /translation="MGLDRVAIVGVGLIGGSIGLALKNRALASVVVGVGRDEATLEGA
                     VRLGAIDRGTTDLVDAVAEAQVVVVCTPVSRIADDVRRAAESAPAQVLVTDAGSSKRQ
                     IVESVERHARSATVFVGAHPLAGSERRGAQNARADLFDGRVCVLTPTPRTRLDRSRLA
                     RNFWGALGCRVLEMSPAEHDEIVAYTSHLPHAVAAALASSVLPAWLPLAAGAYRDGTR
                     VAAADTEIWTAIFRDNRGPLLKALGTLQERLDAFKYALMTDDEEAIRHWWNQARERRN
                     LFDAQPDSPDPTQDPADRFT"
     gene            7181..10024
                     /gene="purL"
                     /locus_tag="VHS97_20960"
     CDS             7181..10024
                     /gene="purL"
                     /locus_tag="VHS97_20960"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX3450738.1"
                     /translation="MDRLGERLALEASESGHDGPWAIRASRGFLIEGQLGEAELERAA
                     RTVLVDPVVEVHSIRPVSARWGEPGTVVHVMPKPGVTDPEAESALALLRDLAYPVAMV
                     RTIRTYRIEGPATGLPRIVARVLANDAVETAFLGSLPFEHLAQGQPYRFQRVQVPLRD
                     LNDQALMAVSRSGQLALSLAEMKTIQRHFALLGREPTDCELETLAQTWSEHCSHKTLR
                     GRISFEGRTIDNLLKQTIFRATHELGCDWLVSVFSDNAGVVRFDQENDVCFKVETHNH
                     PSAIDPYGGANTGLGGVIRDALGTGLGAKPICNTDVFCVASPDLEPEDVPAGVLHPKR
                     VLKGIVAGVRDYGNRMGIPTVNGALAVDPGYLANPLVFCGTVGVLPRGKAHKRVEPGD
                     RIVAIGGRTGRDGIHGATFSSAELTHESESVSGGAVQIGNAITEKMVLDVIIQARDLG
                     LFSAITDCGAGGFSSAVGEMGAELGASVDLEKAPLKYQGLSYTEIWISEAQERMVLAV
                     PPEKWPALLDLCIGEGVEATDLGEFVDSGRLTLRYHGEVVADLSMNFLHEGRPAVVRE
                     AVWTPPEDRPERLPEPRDFTADLLALLSHWDVCSKEWIIRQYDHEVQGRTAIKPLVGI
                     EDDGPGDAAVVMPVRGSTRGLAVGCGINPRYGRIDPYRMAACVIDEAIRNCVAVGADP
                     GRIALLDNFCWGNTERPETLGALVLAAEACRDVALAYGTPFISGKDSLYNEYTHEGRS
                     LAILPTLLISAIGQIDDLRRCVTMDLKEPDNLLLLVGMTRCELGGSLWSHIREHAGGR
                     VPRVEPALGRPLIAALHTAIGRGLVRSCHDLSEGGLAVAIAEMALAGGLGARVDLTEV
                     AHEDDANVDLVILFSESPTRFLIEVTPTCWDEVIEVFRGLPLGRLGRVTGVAGNGKGD
                     SPRLTVHGLDHSVVIDAAVADLKTVWQRPLAWS"
     gene            10126..10959
                     /locus_tag="VHS97_20965"
     CDS             10126..10959
                     /locus_tag="VHS97_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX3450739.1"
                     /translation="MLRFVTFVDGSNLDGVLKHLNLRVDDYGAFYRHVFEQSVQYWGR
                     TFADGAQWPTAQHSRIYWYVVGKMDEWDLNDPKAEARLRTRFDMNPRLRDAYIEDASR
                     RLSDVPLDRRIEEAWNLCFTETREWYESKRRALERKKRFYHGVQAATDFVEIRQEGHW
                     KVDLLHHTVNEKGLDTSLAVDMVALQETYDIALLISGDADGIPGINYVKSRSKHVGVA
                     EFRRGSPADFPTKGTSSRLKIAADFVVQVYEADLLRRNLAYRSETDYSSQLGGPGDLP
                     Y"
     gene            11047..11829
                     /gene="purQ"
                     /locus_tag="VHS97_20970"
     CDS             11047..11829
                     /gene="purQ"
                     /locus_tag="VHS97_20970"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HEX3450740.1"
                     /translation="MASPRVIVIRAPGTNCDEEAVLAWQRAGAVAETWHVNRLIESPA
                     VLDRFEILTIPGGFSYGDDLGAGRILATRLGVVLGDGLRRFHDRGGLVLGICNGFQVL
                     VRSGLLPGGPFPFAATLAHNDSGRFEARWVNVVPRPGLSPFVTFNEPIELPVAHGEGK
                     FLTALPAQLGRLEKNGQIVLQYACAAGRPTQDYPANPNGSAGAAAGVCDSTGRIFGLM
                     PHPERFIESWHHPRWTRRVEGPGGVGDGLLIFRSAVQSLQSS"
     gene            11985..>12227
                     /locus_tag="VHS97_20975"
     CDS             11985..>12227
                     /locus_tag="VHS97_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX3450741.1"
                     /translation="MMPPLTSPSRRAFIGTVVSSAAFFTTRGLFAEQLALTPSRTEGP
                     FYPDRLPLDTDNDLIIVNDGITPAVGEITHLTGRVLD"
BASE COUNT         2306 a         3725 c         3777 g         2419 t
ORIGIN      
        1 tccagcgctc gacggtgtcg cccttgaagg gatacgcatg attggcaaag atcacaccgt
       61 tgcccgtcgg atcggcgagt tgcttcccag tgaggaagcc cgtcatgtcg tacgcccaat
      121 ccagcccacc cgcgacaatg aggtttttcg cgccagtcgc acgtaccgcc tcgagcagcg
      181 ctttcattcc cacggcctgg aaggattttg cggggttgcc gcgatacgcc ttttcctcga
      241 cctggccgcc attcaaccag acgtcccagc tcacgtcgtg cggctcattg tacaggtcga
      301 agatcacggc gggatggttc ttgtagttac tcgcgcagct cttccagaac tccaggctgt
      361 tttggtcggg catcacgtgc tggccgattt gtttgcccca ttcgccagca tccgaccagt
      421 gcagatcgag catgatgtaa cagccctgct gggcgcagaa atccacgatc ttattcacca
      481 gttcgcgata agctttgcct tcgtccttct gttccggggc cttgccgaac cagcgatcct
      541 gcgccagcgg cagacgaatc acattgacgc gccagtcctt gatggcggtc ttgaccgtgt
      601 cgaggatgtg gccctcgccg tcgctcgacc attcgaggga ggcagcgttc acgccgcgga
      661 gacgaactga ttcgtcgtga ctattcacga gcttcgactt ctggaccttg agcggcagcg
      721 gcatcgagga tcgtccatct ggtgttcggg cgagtgcaac cgcggtctgc gccagtacga
      781 caatcccgac accgatccga atcatgcagc gaaccatcga acgtcctccc atttctgatc
      841 ccgaaaaccc gccggaatcg ccacgaccac gccggcgatt ctccggcgta agctcggcga
      901 gtgcaagtga cccgcatagg tcggacaccc atcttcgagt cccctcactg cggttccgga
      961 gtgctggctt cccagcggcg tcggcccagt cgtatagtac gccattgact cgaccggacg
     1021 gtcggttgcg agccattttt ttgcataccc ctcacattgt gcagaagtca cttgaccgcg
     1081 aactggcttg ttccttcttg ttccacagcc ccgtatgcta tcgtgcatta cgccgcgtgg
     1141 ttcgctcacg ctcttgcgcg gtaatgtacc aaatcgatcg accacctgga gggtttcatg
     1201 cacgatcctc gacacaattc gcgccgttcg ttcctcaaat tcgcggcggc ggcctctgct
     1261 gcgtcgactc ttgccaccac ggcacttgcc gccgacggcg tccaagagct caggccgacg
     1321 gtcgagcccc ccaagccggc cggacccaac gaccggatcc agatcgccac gatcggcatg
     1381 ggcatcatcg gcttcatcga tactgcgtgc gcactcaagg tgcccggcgt cgagctggtc
     1441 gctgccgccg acttatatga tggtcggcgg atgcacgcca aagaagagtt cggagacaag
     1501 atacaaacct acgtcgatta tcgcgagatc ctgggccgca aggacgtcga tgccgtgctc
     1561 atctgcgtac ccgatcattg gcatgccagg atgtcgatcg acgcgatgaa ggcgggaaaa
     1621 gcggtctact gcgaaaaacc gatgatccgc ggtgtcgagg aagggccggg cgtgatcgcc
     1681 gtgcaaaagg aaacgaacgc cgtctttcag gtcggcagcc agttcgcgag ctcggtcctc
     1741 tacgacaagg tcaaggacct gatccgcacc ggcgcgatcg gcaagctcca tgcgatcgaa
     1801 gcgcggtata accgcaattc cgacaccggc gcatggcaat attcaattcc taccgacgca
     1861 tccaccgaga caatcgactg ggaccgcttc ctcggcgacg cacccaagcg gccgttcgac
     1921 ccgattcgct tcttccggtg gcggaactac tgggattacg gcacggccgt ggccggggac
     1981 cttttcatcc acctcttgac cggcatccac catgcgaccg gcgcgatcgg cccgacgcgg
     2041 gtcgatgcga tgggaggccg gcgctactgg gaagatggac gcgaagtcta cgacgtcatc
     2101 atgggcctgc tcgactatcc ggagactgac acccacggcg gcttcacgct ctcgctctgc
     2161 accgattttg aagatggcgg aggccctgga accgcgttcc gcttcgtggg caccgatggc
     2221 gtcatcgacg tgagctttaa agagctcact ctcaccctaa tggggatcga gcattcgacg
     2281 cccgatcaga ttctcaatgg gtacaactcg gtcaaaacat tctcgattgc ccagcagaag
     2341 gcattcgccg cgaaactttt ggccgaacat cccagctcgt cgacaaaacg cgctcccaag
     2401 cagaccgaga aattcgtcgt gcccaaaggc tacgacgagc gatttgacca catggtcaac
     2461 ttcttcacgt caatacgcga gcacaagccc gtttatgaag acgcgacgtt cggcttgcgc
     2521 gcggccgcgc cggctctctt gtgcaatgag agctaccgcc gaaagcgcga gatcggctgg
     2581 gatccggtcg aaatgaagat cgtgggatga tgcgcttgcc ggcgcaccga atcggcaggc
     2641 ggctcgcggc gcggccgacg ccaaggctgt ggtacgatct gactgaagaa cccgtgtgca
     2701 ccggcatgtt ttcaggagct gacattcgat gggatcccca gggaataccc ctgcattcgc
     2761 ctcgaatcga gggaatggtc cgtacgcgcc actgcgaagc ttgccgcctc tgcccgaaga
     2821 ttggcggtcg cttcccagag ccctggtgat ccgggccagg gcgcgctggt ccggcgaggc
     2881 gatggccgac agcacggggg cgagcttgac ttatggtcag acgctcattc gcgcgctcgt
     2941 ccttggccga gtcctcgctc gcacctgggg gatcgcggaa cacgtcggga ttctggtccc
     3001 gccgactgtg ccggcggcag tggccaacct ggcggtctcg ctctggggca aggttccggt
     3061 caatctcaac tattcagcca gccaaagtct cgtcgacgcg tcggtggacc aatgtgggat
     3121 cacccacgtc ctgacctcgg ccaaggtgct cgatcggttc aagattactc ccaacgccaa
     3181 attgatcttg ctcgaagaca ttcccaagca ggtgcggctg gcagataagc tttgggccgc
     3241 cgccgccgcc aagctggtac cgttgggcgc cctgggggct ctggttccgg gcctgcgcgg
     3301 cgaccatctg gatgctacgg cgaccgtgat ctttacctct ggatcgaccg gcgaccccaa
     3361 aggggttgtt ctctcgcatc ggaacatcct ctctaacgcc catcagatcg gtgagcaggt
     3421 ttgcatgaag cccgaggagg ttctcctggg cgtattgcct tttttccact cgttcggctt
     3481 taccatcacg atctggactg ccatttgcct taataagaag gtcgtctatc acttcaatcc
     3541 gctcgattcg cgcacgatcg gcaagctctg cgaacaacat cgagtgacgc tcttgacggg
     3601 tacgccgtcg ttcactcgga tgtacctcaa aagttgcccg gcgagccagt tcaaaacgat
     3661 cacccactgg atcgttgggg cggagaagct gaagcccgag ctggcgcgcg acctcacggc
     3721 gacactcaac atcgaacctc tggaggggta cggctgcaca gagctctcgc cggtcgtggc
     3781 ggtgaacgtt ccgcgcgagg tcgagcttcc cggtggccgc aaaatctacg gcaaccgcct
     3841 gggtaccgta ggacttcccc tccccggcac ggcaatcaag accgtgaatc ccgacaccgg
     3901 ggccgacttg ccagtgggcg ccgaagggat gattgctgtc aaagggcccc aggtgatggt
     3961 cggctacctg agccgccccg aggcaactgc ccaggtcctc aaggatggct ggtatggaac
     4021 gggggacctg gggtacctcg atcccgacgg atttctcaag atcaccgatc gaatcagccg
     4081 tttctccaag atcgccggtg agatggtccc acacatcaag atcgagtcgg caatcatcga
     4141 gctgacgggc gtcgacgaga atcatgtcgc ggtcaccgga gtgcccgatc ccaagcacgg
     4201 cgagcgtctt tgtgttctct acaccgacat gggaatctct ccgacagagg tccagcaccg
     4261 cctcaatgcc ggaccgatgc ccaaggtgtg gatcccatca cccagtgact ttgtccaagt
     4321 cgaggcgatc ccgatcacgt ccaccggtaa ggtcgacttg cgcaggctga aggagatcgc
     4381 gctcaccaaa tgtgccggcg aggagatctc gcggcgagcg tgagccgctg tccatccgaa
     4441 aaagtaggcg gcgcccccgg gcgaaaccgc cgccggaacc ggcggcacaa gcgtgtcgac
     4501 gtttgtcgcg cacatcattc cgtgccagcg cgggtttatt ctttgcgagg atctatgtaa
     4561 aactatgagt agtacctggg tagaactctg agggaagcct cgatggggat cgtcctccgc
     4621 gtaacgtcag gccctcatac cggacaggaa ttccttgtcg accattgtgc gacattcacg
     4681 gtcgggcggt cgtcccgcgt ccaattcgcc atggtcgacg atctggcgct ctccagggag
     4741 catttccagg tagacaacga ccctccgttg tgccatctga tcgatctggg gagcaccaac
     4801 ggcaccaagg tcaatgggct gcgcgtcggc cgggcgctct tgcgcgacgg tgacgaaatc
     4861 accgcgggag acagcacgtt cttactccgt ttcgttgggg actcccaaga taccctcaag
     4921 ttcgcaacct gtgcggcatg tggcgcacga gtccctctcg gcttgatcgc aacggatccg
     4981 ctcggcgaag tcctgtcgga cgcgaagggc cccgacgtgt ggctttgcag taactgtcgg
     5041 gcaagccgcc tcaagtttcc caggacccat cccgactacc tgatcgaaga atggatcgga
     5101 ggcgggggca tgggcgaggt ctttcgtgcc cgtcaactgt cgcgcaggcg gcccgtcgcg
     5161 atcaagatga tcagtgcgaa caatgcgctg caagagaagg cgagcagcta ttttcgcagg
     5221 gagatcgagg ttttgagaga tctcttgatg cgcggcggcc agtctcatcc cagcatcgtc
     5281 gccttctacg agatttacga aatcgattcc cagtttcaac tggtgatgga gtacgttgat
     5341 ggcatgaacg cgcttgactg gatcaaagga ctggagaggc ctttgccgac gtccagcggc
     5401 gcgcaaatcg gccgccatct tctgtcggca ctcgactacg cccattcgaa gggatatgtc
     5461 catcgcgatg tgaagccaac caacctgctg gtgatggggc ccatacaccg gccgcgcgtc
     5521 aagctgaccg attttggact ggccaagagt tttgccgaga acggaggctt caccaaactg
     5581 actcgacagg gagacatcgg cggctcagtc ggcttcatct cgcccgacca catccgcgag
     5641 ttcagcgata ttcgggagcc cgccgacatc tattgcgccg ccgcgacgct gtttttcctt
     5701 ctcacaaaca agtatccgta cctgggcttc gacccgcgcg agcgcggctc atacgagatg
     5761 atcctccagc atcctccggt cccactgcgc gcgtttcgcc ctgacgctcc cgaggggttg
     5821 gaaaaagtct tgctcaaggc ccttcaaaag cagccgcgcg accgctggaa atcggcgcag
     5881 gcgatgggcg aggcgttgcg accgtttgcc tcgccgtcat cgtcgtgagg cttgcgaccc
     5941 aatcgcgcgg tcgaacggcg ggccgacggt cgctgcgagt actgccgtat gcaccaagcc
     6001 ctgcttcgat ctgtttccac actaacgctg gcgataacga ggaagcatag ccgcccggcc
     6061 agccgattct cagtcgagca cgcggtggca aagctggcga attcacgtta atatatcgtg
     6121 aagggcacag ggaaggcctg taccgctcgt ctttgtcgtg gggtcgaggg ggtcgtgcct
     6181 tggtgggtct ggacagggtg gcgattgtgg gagtcggtct gatcgggggt tcgatcggcc
     6241 tggcgctcaa gaaccgcgcg cttgcgtctg tagtcgtcgg cgtcgggcgc gacgaggcaa
     6301 cgctcgaggg ggccgttcgg ctgggggcga tcgatcgagg cacgaccgat ctggtcgacg
     6361 cggtggccga ggcccaggtc gtggtcgtct gcacgccggt cagccggatc gccgatgacg
     6421 tgcggcgcgc ggccgagtcg gctcccgcgc aggtattggt gaccgacgcc ggcagctcga
     6481 agcggcagat cgtcgagagc gtcgaacggc acgctcgctc ggcgacggtc ttcgtgggcg
     6541 ctcacccgct cgccggctcc gagcgacgag gcgcccagaa tgcccgggcc gacctgttcg
     6601 acggccgcgt ctgcgtgctc actcccaccc cgcggactcg ccttgatcgc agtcgactgg
     6661 cacgaaactt ttggggtgcg ctgggatgcc gcgtgctcga aatgtctccc gccgagcatg
     6721 acgagatcgt cgcctatacc agccacttgc ctcacgcggt cgcggccgcg ttagcctctt
     6781 cggttcttcc tgcatggctc ccgttggccg ccggcgccta ccgcgacggc actcgggtcg
     6841 cggccgccga tacggaaatc tggacggcaa tctttcgcga taaccgcgga cctttgctca
     6901 aagcgcttgg taccttgcaa gaacggctcg acgcattcaa gtacgctctg atgaccgacg
     6961 acgaagaggc catccgccat tggtggaatc aggccaggga acgacggaac ctcttcgacg
     7021 cccagcccga ctcgccagac ccgacgcagg atccggccga tcgctttacc tgacccgacc
     7081 gtattggact ttgatcgaaa atcgaacttc ctttaaacgt ataactagtt tcattacgtc
     7141 tatgctttgg cacatccaga tcgaaccggc ccctggccag atggatcggc tcggcgagcg
     7201 gcttgccctc gaggccagcg agtcggggca cgatggcccc tgggcgattc gcgccagccg
     7261 cgggtttctg atcgaaggtc agcttggcga ggctgaactt gagcgggctg cccgcactgt
     7321 gctcgtggat ccggtggtcg aagttcactc gattcgaccc gtgagcgcgc gctggggaga
     7381 gcctggcacg gtcgtccatg tcatgcccaa acccggagtg acggatcccg aggcggaaag
     7441 cgcgctggca ctcttgcgcg atctcgcgta tcccgtcgcc atggtccgta cgatccgcac
     7501 gtaccggatc gaaggccccg cgaccggatt gccccgaatc gtcgcgcgcg tgctcgccaa
     7561 tgatgccgtc gagacggctt tcctcggctc gctgccgttc gaacatctcg cccagggaca
     7621 gccttatcga ttccagcgcg tccaagttcc tctgcgagat ctgaacgacc aggcgctcat
     7681 ggcggtgagc cgatcagggc aactagccct cagcctggcc gagatgaaaa cgatacagcg
     7741 acatttcgcc ctactgggtc gcgagcccac cgactgcgag ctcgagaccc tggcccaaac
     7801 ctggagcgag cactgttcgc acaagaccct gcgcggccgg atttcgttcg aagggcgcac
     7861 gattgacaat ctgctcaagc aaacgatctt tcgcgcgacc catgaactcg gctgtgactg
     7921 gttggtgagc gtcttcagcg acaacgccgg ggtggtccgg ttcgatcaag aaaacgacgt
     7981 ttgcttcaag gtcgagacgc acaaccatcc gtcggcaatt gacccgtacg gtggcgccaa
     8041 cacgggatta ggaggcgtga tccgcgacgc gcttggaacg ggactggggg ccaaaccgat
     8101 ttgcaacacg gatgtcttct gcgtggcgtc gcccgatctg gaacccgagg acgttccggc
     8161 cggcgtgctt catcccaaac gtgtgctgaa ggggatcgtg gccggtgtgc gggattatgg
     8221 caatcgcatg gggattccca ccgtcaacgg tgccctggcc gtggacccag ggtacctggc
     8281 caatcccctt gtcttttgcg gcactgtcgg agtcttgccg cgagggaagg cccacaagcg
     8341 agtggagccg ggcgaccgga tcgtggcgat cggaggtcgg accggtcgcg acggaattca
     8401 cggtgccact ttcagctcgg ccgagcttac acatgagagc gagtcggtct cgggtggagc
     8461 ggtccagatc ggcaacgcaa tcaccgaaaa gatggtgctt gatgtgatca ttcaggcgcg
     8521 cgacttaggc ctctttagcg cgatcaccga ctgcggcgcc ggtggcttca gctccgcggt
     8581 gggggagatg ggtgcagagc tcggtgcctc ggtcgatctc gaaaaggcgc cgctcaaata
     8641 ccaagggctt tcctatacgg aaatctggat ttccgaggcg caggagcgga tggtgctggc
     8701 cgttccgccc gagaaatggc cggcactctt agatctgtgc ataggggaag gggtcgaggc
     8761 gacagacctg ggcgaattcg tcgactcggg gaggctcacg ctacggtatc acggcgaggt
     8821 cgtcgccgac ttgtcgatga actttctgca tgaagggagg ccggcggtcg tgcgtgaggc
     8881 cgtctggact ccgcccgagg atcggccgga gcgattgccg gaaccgcgtg atttcacggc
     8941 cgacctgctc gcgctcttat cccattggga cgtttgcagc aaggaatgga tcatccgcca
     9001 gtatgatcat gaagttcagg gacgcacggc gatcaaaccg ctcgtgggaa tcgaggacga
     9061 cggacccggc gatgcggcag tggtgatgcc ggtacgcggg tcgacccggg gcctggccgt
     9121 tggttgcggc atcaaccccc gctacggcag gatcgacccg tataggatgg cggcgtgcgt
     9181 cattgatgag gcgattcgca actgcgtggc cgtgggcgcc gatcccgggc gtatcgcact
     9241 tctcgacaac ttctgctggg gaaacaccga gcggcccgag acgctggggg cacttgtgct
     9301 ggcggccgag gcttgccgcg acgtggcctt ggcatacggc acaccgttca tttctgggaa
     9361 ggatagtctg tacaacgaat acacgcatga aggccgaagc ctggcaatac tccctaccct
     9421 cttgatcagt gccatcggcc agatcgacga tctccgccgt tgcgttacga tggatttgaa
     9481 ggaaccggac aatctacttc tgcttgtggg aatgacccga tgcgagctgg ggggaagttt
     9541 gtggtctcac atccgcgagc acgctggtgg gcgcgttccc cgggtcgaac cggccctggg
     9601 ccgccctttg atcgcggcgc tgcacaccgc gatcgggcgc gggctcgtgc ggagctgtca
     9661 cgatttgagt gagggtgggc tggcggtcgc gatcgcggaa atggcattgg ccggaggctt
     9721 aggcgcgcgg gtcgacctga ccgaggtcgc tcatgaggac gacgcgaacg tcgatctcgt
     9781 catcctcttt tcggaatcgc cgactcggtt cttgatcgaa gtgactccaa cttgttggga
     9841 cgaggttatc gaggtattcc ggggtctgcc actgggccgg ctaggccgag tgacgggggt
     9901 tgccggtaac ggcaaggggg actcgccgcg actcactgtg catggactcg atcacagtgt
     9961 agtgatcgac gcggcggtcg ccgacctgaa aaccgtctgg caacggccgc ttgcctggtc
    10021 gtagttctta tacgcgaaga gattatcaat gttaacgttt gctttgttga tcgtttttta
    10081 cgattggcgg gataattaat gaccaatgcg gccgagggtt caacgatgct tcgtttcgtg
    10141 acttttgttg atggatcgaa cctcgatggc gtgttaaagc atctcaactt gcgtgtcgac
    10201 gattatggtg ccttttatcg ccacgtgttc gagcagagtg tgcagtactg gggccggacc
    10261 tttgccgatg gcgcgcagtg gccgaccgcg caacattcaa gaatctactg gtatgtcgtg
    10321 ggtaagatgg atgagtggga cttgaatgat cccaaggccg aagcgcggtt gcgaacccgc
    10381 ttcgacatga atccgcgact gcgagacgcg tatatcgaag acgccagccg gcgcctgtcc
    10441 gacgtgcccc tggatcgccg catcgaggaa gcctggaacc tctgcttcac cgaaacgcgc
    10501 gagtggtacg agagcaagcg gcgggctctc gaacgaaaga agcggtttta tcacggagtc
    10561 caggctgcca ccgactttgt cgagattcgc caagagggtc actggaaggt cgatctgctg
    10621 catcatacgg tgaatgaaaa ggggctcgac accagcttgg ccgtcgacat ggtcgctctc
    10681 caggagacct acgacatcgc cttgttgata tcgggcgatg ccgatggcat tcccgggatc
    10741 aactacgtca agagccgatc caagcatgtc ggagtcgccg agtttcgacg cggctcgccg
    10801 gcagatttcc ctaccaaagg aacgtcgtcg cggctcaaga ttgccgctga tttcgtggtg
    10861 caggtttacg aggccgatct gctgcggcgc aacctcgcgt atcgctctga gacagactat
    10921 tcgtcgcagc tcggcggccc cggcgatctc ccgtattaaa ccactcggcg ctggctggtt
    10981 cggccgacgg aaccgatcgg aaagtaccgg caagctgacg acctatgcaa catccgggat
    11041 cgatacatgg cttcccctcg cgtgatcgtc atccgtgctc caggaaccaa ctgcgacgag
    11101 gaggccgtcc tggcctggca acgagccggg gccgtggcgg aaacctggca tgtcaatcgg
    11161 ctgatcgagt cgccggcagt cctcgatcgc tttgaaatcc tgacgattcc cggcggtttc
    11221 tcttacggcg atgacctggg ggccggacgg atcctggcca cacggctcgg ggtcgtgctc
    11281 ggcgatggat tacgtcggtt tcacgatcgg ggcggcctgg tcctgggtat ctgtaacggg
    11341 tttcaggtgc ttgtccgtag cggtcttttg cccggcgggc catttccctt cgcggccacc
    11401 ttggctcaca acgattcggg acgattcgag gctcgctggg tcaacgtcgt gccccggccg
    11461 ggcctctccc cctttgtgac atttaacgag ccaattgagc tgccggtcgc ccacggtgaa
    11521 ggtaagttcc tgaccgccct gccggcccaa ctgggtcgcc tcgaaaagaa tggccaaatc
    11581 gtcttgcaat acgcctgcgc agccggccgg ccgactcagg attatccggc caatcccaat
    11641 ggctcggccg gcgccgcggc cggcgtttgt gattcgacgg gccgcatctt tggcctgatg
    11701 ccccatcccg aacgatttat cgaatcatgg catcatcctc gctggacgcg gagggtggag
    11761 gggcctgggg gcgtggggga tggactcttg atctttcgaa gtgctgtcca gtcgttacaa
    11821 agctcttgat gcggatgtcg taccctggca cgagccggac ggacagtgtt gacaaacttt
    11881 tacggagccg ccggcacggc ggagatacaa tccggatcgt gagtcgacgt ccgcagcgaa
    11941 tgagtcgtgg ttcgacgaca tcccaatcgc aaggagtttt ggccatgatg ccgccactga
    12001 ccagccccag ccgccgcgcc ttcatcggca cggtcgtatc gagcgcggcc tttttcacca
    12061 cgcgcggcct gttcgccgag cagctcgccc ttacaccttc gaggacggag ggtcctttct
    12121 atccggacag gctgccgctg gataccgaca acgacctgat tatcgtcaat gacggcatca
    12181 cgccggcggt cggcgaaatc acgcacttga ccggccgggt gctggac
//
LOCUS       DATAQL010001215         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2565809, whole genome shotgun sequence.
ACCESSION   DATAQL010001215 DATAQL010000000
VERSION     DATAQL010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565809"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1523)
                     /locus_tag="VHS97_20980"
     CDS             complement(147..1523)
                     /locus_tag="VHS97_20980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX3450742.1"
                     /translation="MPRRDQAAPRQEAEPKPRLFKGSGAPEPEKHERLQKVLAHAGLG
                     SRRGCEELILQGRVSINGEVIRQLGTRVDRSTARITVDGETIQLESNVYYAVNKPKGY
                     VSTNVDPSGRPRVVDLLPEIPERVYTVGRLDEDSTGLMILTNDGDLANRLAHPRYGVE
                     KLYRALVAGVPTPEMVAKLTEGVWLSDGKVRAKRARIAGRQGQATSLELVLAEGKKRE
                     IRRMLSKLGHKVMALNRIAVGPISLKGLAVGECRPLTRHEIELLQKAAAGASVPPPRF
                     FDGGRSSRPHGESRRPRRPENARSARNHDNSAQHHAQKNENGPPRRQSPHPQNENGPN
                     GVSKRSGNSRPPRPGPSSPGRPSPGSPTARNEPPKLPRHRIDQSNVPAKLRRKGPAKA
                     LPPEPLKIKRRIIGLEIDLRARAGPGAGGGRNRKRPVVRKRRPPGPTLGKARNSSTPT
                     DTSDGDDA"
BASE COUNT          322 a          590 c          639 g          438 t
ORIGIN      
        1 atcggcacat tctccaggat cggcgtccag ctatagcccg cgctggcagg agcaatcgcg
       61 tccatgagag cccaggccct gcaatgcttg agaatgttac cgcaatggct agttcaacga
      121 accggactga ttgctcgacc acacgctcag gcgtcgtcgc catcactcgt atcggtcggg
      181 gttgacgaat tgcgagcttt ccccagagtc ggcccgggag gccgccgctt gcggaccacg
      241 ggccgcttgc ggtttcgacc gcctcccgcg cccggcccag cccgcgcgcg caaatcgatc
      301 tcaaggccga tgatccggcg tttgatcttg agaggttccg gtggaagcgc cttcgcgggg
      361 ccttttcgcc gcagtttggc tggtacattc gattggtcta ttcgatggcg aggaagcttt
      421 ggcggttcgt tgcgtgctgt cggggagccc ggcgaagggc ggcctgggga ggatgggccg
      481 ggtctgggcg gccgcgaatt ccccgagcgt ttggaaactc cgttcggacc gttttcattc
      541 tgtgggtgcg gcgattgccg ccgcggtgga ccattctcgt tcttctgcgc atggtgctgt
      601 gcggagttgt catggtttcg cgcggaacgg gcgttctccg gtctgcgagg acggcgcgat
      661 tccccgtgcg gccggctcga tctaccgccg tcgaaaaaac gaggtggagg cacggaggcg
      721 ccggcggcgg ctttttggag taattcgatc tcgtgtcgcg tgagcggacg gcattcacct
      781 acggcgagcc ctttcaggct gatcggccct accgcgattc ggttgagcgc catgaccttg
      841 tggccgagct tggacaacat gcggcggatt tcgcgttttt tgccttctgc cagaaccagc
      901 tcaagcgatg tggcctgacc ctggcggccc gcgatccgtg cacgcttggc gcgcacttta
      961 ccatcagaca accagacccc ttccgtcagc ttggccacca tctcaggggt gggcacaccc
     1021 gccacgaggg cgcgatagag tttctcaacg ccgtatcgtg ggtgcgccaa ccgattggca
     1081 aggtcgccat cgttggtcaa gatcatcaac ccggtgctat cctcgtcgag acgaccgacg
     1141 gtgtagaccc gctccggaat ctcggggagt aggtcgacca cgcgcggacg gccggagggg
     1201 tcgacgttgg tcgaaacgta gcccttgggt ttgttgacgg cgtaatagac gttggattcg
     1261 agctggatcg tttcgccgtc gaccgtgatt ctggcagtag agcggtcgac gcgcgtgccg
     1321 agctggcgaa tgacttcacc gttgatcgac acgcgccctt gaaggatgag ctcttcgcac
     1381 ccgcggcgcg agcccagtcc ggcatgagcc aagactttct gaagtcgttc gtgcttttcg
     1441 ggctcgggtg cgccgctccc tttgaacaat cgcggttttg gctcggcctc ttgccgcggg
     1501 gcagcctgat cgcggcgcgg cacgctgggc ggtccggggc gccgtttcga gcctggtcgg
     1561 gccccgcgag acttgggcgg tacgttcgat cgagatccct gcgctgggga cgagcggcgt
     1621 cgagccatga tccaaattcc attccagccc ggctgtggcg ggccatcggg cgccatacca
     1681 tcgagggcgc agtatcgtct gtaggtagat tttcatcgta ccgggttggc ggcgatcgtg
     1741 ccagtgcggg tgctcgtgac gcataaagtc cccattagat tgccgtgttg caatccgtca
     1801 acgcattcac aaatcgcgtt gccccgtatc tttccttcct ctggtgtcga aagtagtctt
     1861 ttgaatagac ctcgctgcgc aatgacgagg ttgcacaaca ttgtgtagtc cttgattgat
     1921 tgtcaccaga tatatgcccc ctaggtaatc tcatgcaacg cttccggaaa ctcatgcctc
     1981 tatttgggg
//
LOCUS       DATAQL010001216         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1193442, whole genome shotgun sequence.
ACCESSION   DATAQL010001216 DATAQL010000000
VERSION     DATAQL010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193442"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VHS97_20985"
     CDS             <1..1286
                     /locus_tag="VHS97_20985"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX3450743.1"
                     /translation="EAYQAILRSLGQAAIVFGAGFVLTLLARQRHFLAGSLAVILMTA
                     DLASANALFVLTIPQALFETKPEIVKGIEAAERDDPSPGPFRIHRMPTWHPLGWPDIA
                     SKNRAIELVLWERDTIQPKYGINFGVEYTHTMGVGELYDFDWYFSGFPRNIHDLEVAR
                     RLGVEVEKEVVYFPRRAYDMWNTRYFITPFFPNGWRDENRGHASFLFESDSVLPEPDK
                     FKGPGGAEEAWTWIETQDYKVLRNRQHFPRAWVVHDARMTVPVTGLSADSRSQAMQEI
                     LYGADEIWNDATQVAYDPRKIAWMGNDDALALRPYLSGEVTRPSETVNVTYPNPQQAV
                     LEVSLESPGIVVLADVYYPGWKLTIDGKPAPIYRVNGAMRGAAVPAKSHRVIFTYDPQ
                     SFRIGRLVSVAGLALLVCLGLACAVWPIDPVLGAR"
     gene            complement(1390..>1870)
                     /locus_tag="VHS97_20990"
     CDS             complement(1390..>1870)
                     /locus_tag="VHS97_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450744.1"
                     /translation="SPVVLFGFGTLAGLPVAICAALIASSACRRRWRRLVKLVGLTVL
                     ITVVIGAGWLWFDMRSMPAIEHYERSGWELEILVGAYAVGECAILAWFGREVFRLAWR
                     PQVSGSEQVILGGLSSLADGAPVEVVAAAAEPKVASANPPVNRGPTASAPVAARSTQ"
BASE COUNT          360 a          586 c          554 g          370 t
ORIGIN      
        1 gcgaagcgta ccaggcgatt ctgcgcagtc tgggccaggc cgcgatcgtt ttcggtgcgg
       61 gattcgtgct gaccttgctg gcccggcaac gccacttctt ggccgggtct ttggctgtga
      121 ttttgatgac ggctgacctg gcatctgcca acgcgctctt tgttttgacg atccctcagg
      181 ccctgtttga aaccaagccc gagattgtga aaggcatcga agccgccgag cgagacgatc
      241 cctcgccggg gccgtttcgc attcaccgca tgccgacatg gcacccgctg ggatggcccg
      301 atattgcatc gaagaatcgt gctatcgaac tggttctatg ggaacgcgac accatccaac
      361 ccaagtatgg aatcaacttt ggggtcgagt atacccacac gatgggagtc ggcgagcttt
      421 atgacttcga ttggtatttc agcggttttc cccggaatat tcatgacctc gaggtcgcgc
      481 gtcggctggg cgttgaagtg gaaaaagagg tggtgtattt ccctcgccgt gcctacgaca
      541 tgtggaacac tcggtacttc atcacgccct tctttcctaa cggctggcgt gatgagaatc
      601 gcggccacgc ctcattttta ttcgaaagcg actcggtcct gcccgaaccc gacaaattca
      661 aaggccctgg cggagcggag gaggcctgga cctggatcga aactcaagac tacaaggttt
      721 tgcgcaaccg gcaacatttc ccgcgcgcgt gggtcgtgca cgacgcccga atgacggttc
      781 ccgtcaccgg actttccgcc gacagccgca gccaggccat gcaagagatc ctgtatggcg
      841 ctgacgagat ctggaacgac gcgacgcagg tcgcctacga cccgcgcaag atcgcctgga
      901 tgggcaacga cgatgcgctc gcgttgcgcc cgtatttgtc gggcgaggtc acgcgacctt
      961 cggagactgt caacgtcact taccccaatc cgcaacaggc ggtgctcgag gtcagtctcg
     1021 aatccccggg aattgtcgtc ctcgcagacg tttattaccc gggttggaag ctcacaattg
     1081 acggcaagcc agcgcccatt taccgggtca acggcgcgat gcgcggagcc gcggtcccgg
     1141 cgaaatcgca tcgggtaatt tttacgtacg atccccaatc attccgcatc ggccgactcg
     1201 tctcggtcgc tggcctagca ttactcgtgt gcttggggct ggcgtgcgcc gtttggccga
     1261 tcgatcccgt gttgggcgcg cgctgacgaa aagttgtcct tccggcactg aaacatcccc
     1321 tccattttcg ttgtgcaatt ccggcgagta cagcgaccat gttgcggcgt cggggcggtc
     1381 ggctcagggt cactgcgtgc ttcgtgcggc aaccggggcg gatgccgtcg gaccacgatt
     1441 gaccggcggg ttcgcactcg cgacttttgg ctccgcggcc gccgcgacga cctctacagg
     1501 ggcgccgtcg gcgaggctcg aaaggccgcc aagaatcact tgctcggagc cgctgacttg
     1561 cggtcgccac gcgagtcgaa acacttctcg cccaaaccat gcgagaattg cgcactcacc
     1621 caccgcgtag gccccgacca gaatctccag ttcccagccc gatcgttcgt aatgctcaat
     1681 ggccggcatc gaccgcatgt cgaaccagag ccagccggcc ccgatcacca cggtgatgag
     1741 cacggtcagt ccgacaagct taaccagcct ccgccaacgg cggcggcatg cgctcgatgc
     1801 aattagggcc gcgcagatcg ccaccggcag accagccagc gtaccgaagc cgaataggac
     1861 cacgggggac
//
LOCUS       DATAQL010001217         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5467328, whole genome shotgun sequence.
ACCESSION   DATAQL010001217 DATAQL010000000
VERSION     DATAQL010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5467328"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VHS97_20995"
     CDS             complement(<1..708)
                     /locus_tag="VHS97_20995"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX3450745.1"
                     /translation="MKILSVHPSSLMYSKIFLRLEPLGIELIAAAARRAGHDVRLLDL
                     QVEPGRNYRRALAEWRPDAVALSCNYLANVPEVVELAKAAALLRPGAFVFVGGHSASF
                     IARELLEHGDGAIDCVLKGECESSIGPLLDAALSGDRRDLLKVPGVVAADGTGPPPVY
                     VKSLDDLVAARDLLRHRRKYFIGVLDPCASIELSRGGPWDCSFCSAWTFYGRSYRIKS
                     PDAAVAELKQIKEPGVFI"
     gene            1001..2437
                     /gene="miaB"
                     /locus_tag="VHS97_21000"
     CDS             1001..2437
                     /gene="miaB"
                     /locus_tag="VHS97_21000"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX3450746.1"
                     /translation="MADQPTSHPGKKLYIETVGCQMNLLDSELVVAKLRNEGYELTED
                     LNQADAIFYNTCSVRQHAEDKIYSALGRIKRLKETRPGLTIGVLGCMAQKDQEAIFRR
                     APHVDMVVGPGQLAKVPELLARARDHEPAQVAVSRARTDGSLEVITASFENYDADREP
                     AMRPSPFQAFIRVMMGCDKFCTYCIVPAVRGPEQSRPPTAIVAEARLLADQGVKEITL
                     LGQTVNSYHYKEGDGRLCRLSDLIARIHDVEGIERIKFLTNFPNDMTDDLVAAVSDLP
                     KVSRYLHVPAQSGCDTILKRMKRMYTVGFYENMLARVRESIPDVAVSSDFIVGFCGED
                     DVSFERTVALVKRSRFKNSFIFKYSPRQGTKADGLYRDDVPEAVKKRRNSELLALQNA
                     ISLEDNLRWVGRTVQVLVEGVSRKTARRDGWDGVDQLTGRTACDRIAVFSGTERLIGQ
                     FVRVVVEDASAVTLFGRVETSERTATQA"
     gene            2449..3195
                     /locus_tag="VHS97_21005"
     CDS             2449..3195
                     /locus_tag="VHS97_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX3450747.1"
                     /translation="MAAPLEELVSCWIDWGGIDVRFVPDPVTLERGLAGSKDCCRPGA
                     DPRVIADWEDRHGYRLPAGLRAWLMLSNGLFGVGPLIHPISAIGPMIPFARVPDMIVQ
                     PESWFELGNPNLQTVCIDLGYHLPGGGCPIFTSGDDASHSPPRIIARCFEEWFLELLR
                     HGGREYWFDPEFADFGDPWQTHRYHVANPPLSGRLRSFADRVEQLLRSGADEREIASG
                     LGLSRTEVELIFRHLQHVGPAAVQSRSGGI"
BASE COUNT          713 a         1044 c         1194 g          698 t
ORIGIN      
        1 gataaagacc cccggctcct tgatctgttt cagctcggcc acggccgcgt cggggctctt
       61 gatccggtag ctgcgaccgt agaacgtcca ggcgctgcag aacgagcaat cccaggggcc
      121 cccccggctc aactcgatgg atgcacacgg gtcgaggacg ccgataaagt atttgcggcg
      181 atgacgtaag agatcgcgtg cggccaccag gtcatcaagg ctcttgacgt atacaggcgg
      241 tggtccggtt ccgtctgccg cgacgactcc cggcactttc aagaggtcgc ggcggtcacc
      301 ggatagggcg gcgtcgagca aaggtccaat ggaagactcg cattcgccct tgagcacgca
      361 gtcgatcgcg ccgtcgccat gttccagcag ctcacgggcg atgaacgacg cactgtggcc
      421 gccgacgaac acaaatgccc ctggccgcaa cagggcggct gccttggcca gctcgaccac
      481 ctcgggtaca ttggccaggt aattgcacga taaagcgacg gcgtcgggac gccattcggc
      541 aagtgcgcgg cgataattcc gtcccggctc gacttgcagg tcgaggagcc gcacgtcgtg
      601 gcccgcccgc cgcgcggccg cggcgatcag ctcgatcccc agcggctcga gcctcaagaa
      661 gatcttggag tacatgaggg agctaggatg gacggagaga atcttcatgg gcggaaaaca
      721 acatcgagtc aggggtttgg ccaattcgtg gttctgcttg tagcacgaaa tatcaagatg
      781 gaccagcaga agtgacccgg tagagccagg tttggacagt ttcgcgtagc attggccgtg
      841 tcgttggccg aagggttttc ccatcctatc ttgtttgccg agcgatccgt gctgcgtggg
      901 cggcgtcggt gcatatacgg tcgatcagaa gtggtattca gatcggtggc gctttgttac
      961 aatccgcttt tgagtttgag atcgtcgcgg agccgacatg atggccgatc aaccgacctc
     1021 acaccccggc aagaagctgt atatcgagac ggtcggctgc cagatgaacc tgctcgacag
     1081 cgagctcgtc gtagccaagc tgcgaaatga aggctacgag ctcaccgagg atctcaatca
     1141 ggccgatgcc atcttctaca acacgtgctc ggtgcgccag cacgcggaag acaagattta
     1201 cagcgccttg gggcggatca agcgcctcaa ggagacgagg cccggcttga cgatcggagt
     1261 gctgggctgc atggcgcaaa aggatcaaga ggcgatcttc cggcgtgcgc cccacgtcga
     1321 catggtcgtc ggccccggcc agctcgcgaa ggtccccgag cttttggcca gggcgcggga
     1381 tcacgagccg gcccaggttg cggtcagccg tgcccgaacc gacggatcgc tcgaagtgat
     1441 cacggccagc ttcgagaatt acgacgccga ccgtgagccg gcgatgcgac ccagcccgtt
     1501 tcaagccttt attcgggtca tgatgggctg cgacaagttt tgcacatact gcatcgtccc
     1561 tgcggtgcgc ggtcccgagc aaagccggcc gcccaccgcg atcgtcgccg aggcacgatt
     1621 gctggccgac cagggggtca aggaaatcac cctgctgggc caaacggtca acagctacca
     1681 ctacaaggaa ggtgacgggc gattgtgccg cctctcggac ttgatagcgc gaattcacga
     1741 tgtagaaggc atcgagcgga tcaagtttct caccaacttt cccaatgata tgaccgacga
     1801 cttggtcgcg gccgtgagcg acttgccgaa agtcagccgc tatctccatg tgccggcgca
     1861 gagtggctgc gatacgatct tgaagcgaat gaagcgcatg tacacggtcg ggttttatga
     1921 gaacatgctg gcccgggttc gtgagtcgat cccggacgtg gcggtctcaa gtgatttcat
     1981 cgtcggattt tgtggagaag acgacgtttc gttcgaacgg acagtggctt tggtcaagcg
     2041 gtctcggttc aagaatagtt ttattttcaa gtacagtccc cggcagggga ccaaggccga
     2101 cgggctgtac cgcgacgatg tgcccgaggc ggtcaagaag cggcggaaca gcgagctatt
     2161 ggctttgcag aatgcgatta gcctggagga taacctccgc tgggtcgggc ggacggttca
     2221 agtcttggtc gagggcgtca gtcgcaagac ggcgcgtcgc gacgggtggg atggagtcga
     2281 ccagttgacc ggacggacgg catgcgatcg aatcgcggtg ttctccggca ctgagaggct
     2341 gatcggacaa ttcgtgcggg tcgtggtcga ggatgcgagc gcggtgacac tattcggtcg
     2401 ggtggaaaca tccgagcgca ccgcgactca ggcgtgaagg gatgtgcgat ggccgctccg
     2461 ctcgaggagc tggtaagttg ctggatcgat tgggggggga tcgacgtccg ctttgtcccc
     2521 gatccggtca cgctcgagcg cggattagca gggagcaaag actgctgccg gcccggcgcc
     2581 gatcctcggg tgatcgccga ctgggaagat cggcacggct atcgtttgcc ggccgggctg
     2641 cgcgcctggc tgatgctctc taacggcctc ttcggggtgg gtcccttgat ccatccgatc
     2701 tccgcaatcg gcccaatgat tccctttgcg cgcgttcccg acatgattgt tcagcccgag
     2761 agctggtttg agctgggcaa ccccaacctc cagacggtct gcatcgacct gggataccat
     2821 ctgccgggag gcggctgtcc gatcttcacg tccggcgacg atgcgtcgca cagtcctccg
     2881 cggatcatcg cccgctgttt cgaggaatgg tttctcgagc tattgcgcca tggggggcgg
     2941 gaatactggt tcgacccgga atttgccgac tttggcgatc cgtggcagac gcaccgttat
     3001 cacgtcgcca atcctcctct gtcgggccgg ttgcggtcgt ttgccgaccg tgtcgaacag
     3061 ctcttgcgct cgggggccga cgagcgcgaa atcgccagcg gccttggcct ctcgcgcacg
     3121 gaggtagagc tgattttccg ccacctgcag cacgtcggcc cagccgcggt gcagtctcga
     3181 tccggcggga tttgaggaaa tttcgttggc tttcgcacga actgcaaggc cgcgatgaac
     3241 gaatacgggt tgatcggggg gatgacgacc ggcaagctca ggtcggagac ctccgtggtg
     3301 cttcacactc ggagggcctg agtgctgcgc cacggaaatg gggattggaa ttcgggagga
     3361 cgaggctccg gccgagccac ttcttgatgc tggctcgcac ggaggcgagc ctggcgcggt
     3421 gaaaagttgg tattgacagg ccggagcgct tgttcccgtt tcattgatgg gcgggagccc
     3481 gaaaagcccc gaatggggcg acatattgca gcctggggtg caaacccaag gtatcgacga
     3541 aaccaacatt aaacgatgac agaatccagt acaccgtcac gacccaggac ggggtcccga
     3601 gtcgtgacgg tgtatctcga ggagacgcgg cgcgtggccg cctacgaac
//
LOCUS       DATAQL010001218         4135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1955902, whole genome shotgun sequence.
ACCESSION   DATAQL010001218 DATAQL010000000
VERSION     DATAQL010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955902"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1435)
                     /locus_tag="VHS97_21010"
     CDS             complement(80..1435)
                     /locus_tag="VHS97_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450748.1"
                     /translation="MNIQHLYKNEMQVVGGEKSPVSFLSRFRQGRVPADAPRRRRRAV
                     RPGFDPLEGRQLLSLGAGSWQVSVTDGGTKNGSANANAPNGAEAIAWVDNVNGYSEVV
                     VRAFNTAKQAIGGEIRVDYSKAEYSVDDSVGVAIGNNGDFAVVWRNYNFLSHVSSVYV
                     EQFGPSGNPIALNRVGDSAYGFSDFTPDVAIDQSGNVLVSFVESINSRQEVVDVEEIN
                     AAGHMGVTLYVNNNPGFTFIDTKLAMSSNGSFAVGYDFTGPGYDGDWDLTLVRYPAGA
                     SRPNVAPIIDIQQGSPVDFAISANSGGIFAVAMVDKNSNGTSKLDVTQVDNAGSSTAK
                     TVIDNTGHTLYHPAIALANNGAYVVAYDTTSVLGRPGTLGVDIVEVNSAGSIGLALLD
                     AYGREVIDPALSIDSYGNYIATFTGADGEIRDRGGLLPQIRYIPITPIPIVTLPGGLK
                     I"
     gene            complement(1702..2286)
                     /locus_tag="VHS97_21015"
     CDS             complement(1702..2286)
                     /locus_tag="VHS97_21015"
                     /inference="COORDINATES: protein motif:HMM:NF024406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEX3450749.1"
                     /translation="MMHNIGKCAVVSFIGNIRGDSEARSRRLVVNEQRSTPDQLLLYA
                     QDILVQAEPMAPAPQPPDRAAPNSRSWNHGRKPLPANLPRRGVVHDVAPEDRACPECT
                     GQRRPMGEEVREQLEYVPASLIILEHVRPKYACAGCAAHVVIAARLPEPIEQGLPGTG
                     LLAHVIRSKLRSRSPARASPSLESIPGEKHFRPA"
     gene            2700..3290
                     /locus_tag="VHS97_21020"
     CDS             2700..3290
                     /locus_tag="VHS97_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450750.1"
                     /translation="MQRRQSRVPPGPTVAATVRRVEEGRDRARSHPGRHHRIRKDSAD
                     VGIDKDYPHRRHTQTLVRSLSLTESQLMDEAVRRRSRFYRGREIELADDQPWILPSRG
                     VESEADFTRLGPDYRSLLRAVREAGDEAERRLAELALAIFLLGFNYVLSPIDYQRLLV
                     FPPGASELVEVQEEFHRLAQDHLGYLPDDHGGNSGT"
     gene            3904..>4135
                     /locus_tag="VHS97_21025"
     CDS             3904..>4135
                     /locus_tag="VHS97_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450751.1"
                     /translation="MDRRGGDAGRHRLSHSVDSCTKDHHEEDGDRHRLYTGPPRRDEM
                     DSMIHGSPASRFELDHLEFLRWSRQYQLHHEMR"
BASE COUNT          712 a         1313 c         1374 g          736 t
ORIGIN      
        1 gggtaacgtc tttgttaccc cggtcattgg gcgaccgggg tggggtcccc ggccaactgc
       61 cgcgcgccgg gttcggccct cagatcttca acccgccagg gagagtcacg atggggatgg
      121 gcgtgatcgg gatgtatcgg atctgcggga gcagcccgcc gcgatcccgg atctccccat
      181 ccgcgccggt gaaggtcgcg atgtagttcc cgtaggagtc gatgctcagg gccgggtcga
      241 tgacctcccg accgtaggcg tccagcagcg ccaggccgat cgagccggcc gaattgacct
      301 cgacgatgtc caccccgagg gtaccgggtc tgccgaggac ggaggtggta tcgtaggcca
      361 cgacgtaggc gccgttgttg gccagggcga tggcggggtg gtagagcgtg tggccagtgt
      421 tgtcgatcac cgtcttcgcg gtagaggatc cggcgttgtc gacttgcgtg acgtcgagct
      481 tcgaggtgcc gttgctgttc ttatcgacca tggccaccgc gaagatgccg ccggagttgg
      541 ccgagatggc gaagtcgacc gggctacctt gctggatatc gatgatcggg gcgacattcg
      601 gcctcgaggc gcccgccggg tatctcacca gggtaagatc ccaatcgcca tcatagcccg
      661 gaccggtaaa gtcgtagcct acggcgaagg agccgttcga gctcatcgcc agcttcgtgt
      721 cgatgaaagt aaagcccggg ttgttgttga catagagggt cacgcccatg tggccggcgg
      781 cattgatctc ctcgacatcg acgacctcct gcctcgaatt gatggactcg acgaaggaga
      841 cgaggacatt tccggactgg tcgatggcca cgtccggcgt gaagtccgag aagccgtacg
      901 ccgagtcgcc gacccggttc agggcgatgg ggtttccgct ggggccgaac tgctcgacat
      961 agacggagct gacgtggctc aggaagttgt aattccgcca gacgacggcg aagtcgccgt
     1021 tgttgccgat cgcgaccccg accgagtcgt cgacgctgta ctcggccttg gagtaatcga
     1081 cgcggatctc gccgccgatc gcctgcttgg ccgtgttgaa ggcccggacg acgacctcgc
     1141 tgtagccgtt gacgttgtcg acccaggcga tggcctcggc gccgttcggg gcgttggcgt
     1201 tggccgagcc gttcttggtg cccccgtcag tcacgctgac ctgccaggag ccggcgccca
     1261 gggaaaggag ctggcggccc tccagggggt cgaagccggg gcggacggcc ctgcggcgcc
     1321 tacggggggc gtcggcggga acgcggcctt gccggaaccg actcaggaag gagacgggcg
     1381 atttctcccc cccaacgacc tgcatctcat ttttatacaa atgctgaata ttcatctgtc
     1441 attcctctcg aagaaaaatg ggaacagtga cttcgtccgc gaccggacca ccccggcgcg
     1501 aggagcgaca ctcggctcct cgggcccgat cgagcgttcc cccggcaatc gcagcccggg
     1561 atgacgcgtg cgagccccgc ggattccaga ggtctccagg cctgcttgat cggccagcct
     1621 gtttatcgaa cgagcgacgc gagcgacccg cgtactcgtc gtgatctctc accattcatc
     1681 taacggaatt ccgctcggag attacgcagg ccggaaatgt ttttcgcccg ggatggattc
     1741 cagtgagggc gatgcccggg ccggtgacct cgacctgagc ttgctgcgga tgacgtgggc
     1801 cagcaggccg gtgcccggca gtccctgctc gatcggctcg gggagtcgcg cggcgatcac
     1861 gacgtgcgcc gcacagcccg cgcaggcata tttcggtcgg acgtgttcga gaatgatcag
     1921 gctggcgggc acgtattcga gttgctcacg gacctcctcg cccatcggcc ggcgctggcc
     1981 ggtgcattcg ggacaggcgc ggtcttcggg ggcgacgtca tggacgaccc ccctgcgggg
     2041 caggttcgcc ggcaacggct tgcggccgtg gttccaggat ctggagttcg gcgcggcccg
     2101 gtcggggggc tgaggcgcgg gagccatcgg ctcagcctgg acgaggatgt cttgcgcgta
     2161 gagcaggagc tgatcggggg ttgatcgttg ttcatttaca acgaggcgtc gcgaccgggc
     2221 ctctgaatcg cccctaatgt ttcctatgaa cgacactacc gcgcacttgc cgatattgtg
     2281 catcatttat ggatcacggc catcgaatgc cgtagtctca agtcgtggag aggcctcctg
     2341 agctgtttgc aagtaaagga acctgccggc tgccgagtcc aattcaggcc actggtccgc
     2401 gggacttatg tccggcttca tctagcacgc atcgaaacaa ggaaaacggc gtgagagaca
     2461 gagctcgaag cacacgactc gccacggccg ggctgccgat cgccgccctg ctcgtggcat
     2521 tcctatcgtc cccggcacga ccatgcgcag cggaatgccc ggcaagaaag cgatgacgat
     2581 gccaaagccc agcaagaggg gtgcgccgac gccgggttga ggcccttcga tctggatcat
     2641 cccgagcaag aacctatgca ggaacgcgca ccaagccatt aaacagggat gaactgctca
     2701 tgcaacgacg acaatcgcgc gtgccccccg gcccgaccgt cgccgccacc gtacggcggg
     2761 tggaggaggg gcgcgatcga gcgcgatcgc atccgggccg acatcaccgg attcgtaagg
     2821 attcggccga cgtcggcatc gacaaggact atccgcaccg ccggcacacg cagacgctcg
     2881 tgcgatcgct ttcgctcacg gagagccagt tgatggacga agccgtgagg cgccgtagcc
     2941 gcttctaccg aggccgggag atcgagctgg cggatgacca gccttggatc ttgccttccc
     3001 ggggggtcga gtcggaagcg gacttcaccc gcctcggtcc cgattatcgg tcgctcctcc
     3061 gagctgtccg ggaagcgggc gacgaggcgg agaggcgcct cgccgaactg gccctggcga
     3121 tcttcctcct cggattcaac tacgtgttgt cgcctatcga ttaccagcgg ttgctcgtct
     3181 tcccgccagg cgcgtccgag ctggtcgaag tgcaggagga attccatcgg ctcgcccaag
     3241 accacctcgg ctacctcccc gatgaccatg gcggcaacag tgggacgtag gtaccgatcg
     3301 acttgggggc tcggctgctc gaacgctggt tccggagacg tgcgagctaa ccggccgggt
     3361 gatgtttcct tttctcgagg ccctcgagcg gggccaccga gtgcttcggg tgtcgactcc
     3421 ctccgcccca tgacttgctg gggcaggggg acgccgcgtg cggcctagcc gccgtgtcga
     3481 ttgcgtgcaa catgacccgc gtttcgcggg cgtcccgggt caccctgcac gcggcagttc
     3541 gtctacactc ttgaccgcct cctttgggcg acgaggctcg gcaagccgcc aaacgtgccg
     3601 caccgacaag gtgccgtgac cggagtcccg atcccctggc cagatcacac tggatcatgc
     3661 cgcgcgccga tgccgcctgc ggcagggcgt tccaccccaa ccgctcgacg gccgagacct
     3721 atcgcatcgt cttggcgttc cggtaccggg ccaccggcga cggcgcgggc tgctggctgg
     3781 ccgtttaccg ctgcagcggt gcttcgggct ccacatcgcc cgcaagaggg taggccacgg
     3841 gcccgtgggg cccgatcgtt tcccgccgcc ccacgagatg tcgcaggact ggtcggagcc
     3901 cccatggatc gacgcggagg cgatgcggga cgtcaccgcc tgagccacag tgtcgatagt
     3961 tgcaccaagg atcaccacga ggaggacggc gatcggcacc gactgtacac ggggcccccg
     4021 cgccgcgacg agatggattc aatgatccac ggctctccgg catcgcggtt tgaactcgat
     4081 cacctcgaat tcctgaggtg gtcgcgacag taccagcttc atcacgagat gcgcc
//
LOCUS       DATAQL010001219         6145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2721737, whole genome shotgun sequence.
ACCESSION   DATAQL010001219 DATAQL010000000
VERSION     DATAQL010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721737"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5154)
                     /locus_tag="VHS97_21030"
     CDS             complement(<1..5154)
                     /locus_tag="VHS97_21030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HEX3450752.1"
                     /translation="MASAASRKYRPVRTPTILQMEAAECGAAALGIILEYHGRYVPLD
                     VLRDDCGVSRDGSNAFYIKEAARRYGLEVNAFRKPADGLSNRRPPFVVFWEWNHFLVV
                     EGFRRGKVYLNDPASGRRSIGFDEFVRGYSGIAFTFELGPQFQKKGKRPSAVLGLVRR
                     LSTSKTALVFVILAGLALVIPNLISAAFQRVYLDEILIEEHHEWLKPLLWAMALTAAF
                     RLAAAALEQVYLTRLEVRLTLVESIEFMWHVLRLPLAFFQHRWTGDVVSRAASTARVA
                     RLISGDLATTTVSLLTLAVYVAVMLPRDPLLAAVGVGISSLNLVAIQWFGRWRADRNR
                     TIEQIRGRLLAGVMWAIQIIESVKAAGSESDLLVRWSGEQARMINAEQTLGFCDTILL
                     VLPSFLASLTAVVVLGLGGREVILGSLSIGLLVAFQSLLANFNQPFSDLARLGSEVQE
                     LRADLDRIDDVRHRPVDPVFATVNSTPPYAGGTLAEVNASHGRRLSGHIEFRQVTFGY
                     NRTVEEPLIKEFSFVARPGQRIALVGSSGSGKSTIGRLAAGLYAPWSGEILYDGKPLA
                     ALPRAVFVSSVEMVDSEICLFEGSVRDNLTLWDEMVSTERLVQAGVDAAIHGDLLQRR
                     GGYSAPVAEQARNFSGGQRQRLQIARALARDPSILILDEATSALDPKTEMIVDDNLRR
                     RGCTCLIIAHRLTTIRDCDEIIVLSAGRVVQRGTHDELFAASQGEYARLVSHQALPAP
                     RSSRFSLTRRPAAEQSSVPFRGDRSAEISTWSVAPTIDGAVPFFDQDSEPARFIVEEL
                     LPYSRPERTAANLPLALDDAEAVWWVCSGSVDVFFTQFEPGANEGRRRHLCRVDEGGS
                     IFAISGVRGRLGGGLLAVGAGPAQLLKFGRGDLIRLSFEEGLSEQVAILIDDWVIRVG
                     RALARSAGSQSRKELEPGAVAEFKSETRYGARQGVAWIRHLLGSSMFLDRVALHAGEI
                     EARFPVTEHLWLTTATSCRVSVCDTTAMIRTGDPWAGLDDFHRAILDFTALLNDQETH
                     ARWVEFERSTAHEAALVESASSRLAATANKTSLSAVDPGGDALVTACVAVGQVLGIDV
                     RSRLSGAQSLIGPSNDPLGDLARTGGFHVRPVVLPEGWWRHNGGDPLLGRMVDNGQAP
                     VALVPVRSGRDWFGPAYEIHDAQGCRHPVDFDVAQRVAPTAWMFYKTLPDRALGRSDL
                     VRFSLSLPGLRHELVMMIAMAVLSALLGLSIPIASGIIVDQVLPQADFSQLAVVCMFL
                     VVMIAAAAIFQAIQGLMVLRIEGRVSAALVPAFWDRLLRLPSRFFAQFSSGDLALRAM
                     ELSEVFKKVSGAVVATVVTGFFSFFNLALLFVYSWKMAVCTSFLLAILFAVTALLLGG
                     LLRHESAIRAIDGSISGLLLELLGGLSALRTGGAEGRAFARWARRYSERLARAIRARR
                     FSSGIHQWFAVYPILSAMVVYIGAIHIDPNLLKAGSFLPFNIAFVNLMADVLAVGYTS
                     IGLLELIPRYERIKPILVEQPEFSAALIEPVRLMGALALNHVSFRYPGQEKGAKVLDN
                     VSLQVRPGEFVAIVGPSGSGKSTLMRLLLGFEIPDVGAVTYDGRELATLDPRDVRRQI
                     GVVLQHAQLMPSDLFNNIVGFTPLLTMDDAWAAARLAGLEHDIRRMPMGMYTLVGEAG
                     GNLSSGQRQRLLIAQAIVRRPKILLLDEATSALDNVTQAIVSDSITEQLK"
     gene            complement(5325..>6145)
                     /locus_tag="VHS97_21035"
     CDS             complement(5325..>6145)
                     /locus_tag="VHS97_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450753.1"
                     /translation="ADRLRAQKYLGDMELLESREKLYVIKDDLNKGQSRLAELDLEAT
                     TTDYARKRAQLERRLKIRQLKTKLALDCDKLSRTSRVVSKVHGHVAEVLSARGELVHE
                     GSPVVLLHTPRVDLGIAEAGPLYDSILFVPAAEGKKIGLGDDVEVSPTTVKREEHGFI
                     KGKVVAISELPATRLAMEATLEHPQLVDAFLRRYAPGVLLRVDVKLNERATASASVAS
                     PLQPVRGNRFEWSSSSGRAQSLKTGTMCKAAIVVEKRQLISLILPWTKRVVGAE"
BASE COUNT         1245 a         2004 c         1860 g         1036 t
ORIGIN      
        1 cttcaactgc tcggtgatgc tgtcgctgac aatcgcctga gttacgttat caagcgcgct
       61 tgtcgcctcg tcgagcaaga ggatcttggg acggcgcacg atcgcctggg cgatcaataa
      121 ccgctgccgc tggccgctgg agaggtttcc ccccgcttca cccacgagag tgtacatccc
      181 catgggcatg cgccggatgt cgtgctcgag gccggccaac cgcgcggccg cccaggcatc
      241 gtccatggtc aggaggggag tgaacccgac gatgttattg aacaggtcgc tcggcatgag
      301 ctgggcatgt tgcaggacca cgccgatctg ccgccgcaca tcgcgcggat cgagagtggc
      361 cagctcgcgg ccgtcgtagg tgaccgcgcc cacgtcggga atctcgaacc ccagtaagag
      421 acgcatcaaa gtcgacttcc ccgagcccga tgggccgaca attgcgacga attcgcctgg
      481 gcggacctga agactcacgt tgtcgagaac cttcgcccct ttctcctggc cgggataacg
      541 gaacgaaaca tgatttagcg ccaaagcgcc catcaaccgg accggctcga tcaaggccgc
      601 ggagaactcg ggctgctcga ccaggatggg cttgatccgc tcataccgag ggatcagctc
      661 caggaggccg atcgaggtgt agccgacggc cagcacgtcg gccatgaggt tgacaaaggc
      721 gatgttaaac gggagaaagc tgccggcctt gagcaaattg gggtctatat ggatggctcc
      781 gatataaacg accatggcac tgaggatcgg ataaacggcg aaccattgat gaataccgct
      841 cgaaaagcgc cgcgcacgta tcgcccgcgc gagccgctcg ctgtagcggc gtgcccagcg
      901 cgcgaaggcg cggccctcgg cgccgcctgt tcgcaacgcg cttaatccgc ccaatagctc
      961 caggagcagt ccggagatcg atccatcgat ggcgcggatt gcgctctcgt gacggagcag
     1021 cccccccagt aagagcgccg tcacagcgaa caagatggcg agaagaaatg acgtgcagac
     1081 ggccatcttc caactataaa cgaagagaag cgccaggttg aagaacgaga agaaacctgt
     1141 gactacggtg gccacgaccg cgccggagac cttcttgaat acctcgctca actccatcgc
     1201 cctcaatgcc aggtcgcccg aagaaaattg ggcgaaaaac cggctgggga gccggagcaa
     1261 acgatcccag aacgcgggaa ccaaagccgc agagacgcgc ccttcgatgc gcaagaccat
     1321 gaggccctgg atcgcctgga aaatcgccgc cgcggcaatc atcaccacga ggaacatgca
     1381 gacgacggcg agctggctaa aatcggcttg gggcagcacc tgatcgacga tgatgcctga
     1441 agcaatggga atcgacaaac ccagtagtgc gctcaaaacg gccatggcga tcatcatcac
     1501 gagctcgtgc cgtagccccg gtaggctcag gctgaaacga accaggtcgg accggcccag
     1561 cgcccgatcg gggagcgtct tgtagaacat ccaggctgtg ggagcgacgc gttgggcgac
     1621 gtcgaaatcg accgggtgcc ggcaaccttg tgcatcgtga atctcataag ccggcccaaa
     1681 ccagtccctg cccgacctga ccggcacaag tgccacgggg gcctgtccgt tgtcgaccat
     1741 gcgtcccaag agcgggtcgc ccccgttgtg tcgccaccac ccctcaggga gcacgaccgg
     1801 acgcacgtga aaaccgccgg tccgcgccag gtctccgagt ggatcattcg atgggccgat
     1861 gagactttgc gccccagaca gtcgtgagcg gacatcgatg cccaagactt gtcctacagc
     1921 cacgcacgcc gtcacaaggg cgtcgccgcc aggatcgacc gccgaaaggg acgtcttgtt
     1981 ggccgtggcg gccagccgcg acgaagccga ttcgaccagc gccgcctcgt gcgcggtcga
     2041 ccgctcgaat tccacccagc gcgcgtgcgt ttcctgatcg ttcaaaagtg cagtgaaatc
     2101 aagaatcgcg cggtgaaagt catcgaggcc cgcccaggga tcacccgtgc gaatcatggc
     2161 ggtggtgtcg cagacgctca cccggcatga ggtcgccgtc gtcagccaga ggtgctcggt
     2221 cacgggaaaa cgtgcttcga tctcccccgc gtgcaaagcg actcgatcca ggaacatcga
     2281 agaacccagc agatggcgta tccatgccac gccttgacgt gcgccgtaac gagtctcgct
     2341 cttgaactcg gcgacggcgc cgggctccag ctcttttcgg gattgcgaac ccgccgaacg
     2401 tgctaaggcc cgtccgacgc ggatcaccca gtcgtcgatc aagatcgcaa cctgttcgga
     2461 tagcccttcc tcgaagctca gccggatcaa gtctcccctg ccgaacttca agagctgcgc
     2521 cgggcccgca ccgacagcca gcaagccgcc gcccaatcgc ccgcgtacgc cactgatggc
     2581 aaagatcgaa ccgccttcat caactcggca gagatggcgc cgccggcctt cgttggcgcc
     2641 gggctcgaac tgggtgaaaa agacgtcgac actgcccgag cagacccacc agacggcttc
     2701 ggcatcgtcg agcgcgaggg gaaggttggc cgcggttcgc tcgggccggc tgtagggcaa
     2761 gagctcttcc acgatgaacc gcgccggctc tgagtcttga tcaaaaaaag ggactgcccc
     2821 gtcgatggtc ggagcgaccg accaggtcga aatctccgcg ctccggtcgc cccgaaacgg
     2881 cacgctcgat tgttccgcgg ccgggcgtct tgtcagggaa aaacggctcg aacgcggggc
     2941 cggcaacgcc tggtgcgaca cgagccgcgc gtattcacct tggctcgccg caaagagctc
     3001 gtcgtgggtc ccccgctgga ccacgcgccc ggcggagagg acgattatct cgtcgcagtc
     3061 tctgatggtc gtgagccggt gggcgatgat caggcaggtg cagccgcggc gcctcaggtt
     3121 gtcgtcgacg atcatctcgg tcttggggtc aagcgcgctt gtggcctcgt cgagaatcaa
     3181 gatgctggga tcgcgcgcca acgcccgggc aatttgcagc cgctggcgct ggccgccgga
     3241 aaagtttcgc gcctgctcgg cgacgggcgc gctatatccg ccccggcgct gcaagaggtc
     3301 gccgtggatc gccgcgtcga cgccggcctg caccagccgc tcggtcgaaa ccatctcgtc
     3361 ccaaagagtc aggttgtcgc ggaccgaccc ttcgaacaga cagatctccg agtcgaccat
     3421 ctcgaccgag ctgacaaaga ccgcgcgcgg cagcgcggca aggggcttgc cgtcgtagag
     3481 gatctcgccg ctccaaggcg catacagccc ggcggctagc cgtccgatcg tggattttcc
     3541 gcttcccgaa ctgcccacga gcgcgatccg ctggccaggc cgtgccacaa acgaaaactc
     3601 tttgatcagc ggttcctcga ccgtccggtt atagccaaag gtcacctgcc ggaattcgat
     3661 gtgcccagag agccgtcgcc cgtgagatgc gttgacctcg gccagcgtgc cgccggcata
     3721 gggcggagtc gagttcacgg tcgcgaagac cgggtcgacc ggccggtggc gaacatcgtc
     3781 aatgcggtcc aagtcggcgc ggagctcctg gacctcggac ccgagacgcg ccagatcgct
     3841 gaatggctgg ttgaaattgg ccagcaacga ctgaaatgcg accagcagcc cgatgctcag
     3901 actgcccagg atgacttccc ggccgcctaa gcccagaacc acgaccgcgg tcagacttgc
     3961 caggaacgag ggcagaacta ataagattgt gtcgcaaaat cccagcgttt gctcggcatt
     4021 gatcatgcgc gcttgctcgc cgctccagcg gaccagcaga tccgactcgg agccggccgc
     4081 cttgacactc tcaatgatct ggatcgccca catcacgccc gccaagagcc gtccgcggat
     4141 ctgttcgatg gtccgatttc ggtcggcccg ccagcggcca aaccactgga tggcgaccag
     4201 gttcagactg ctgatcccca ctccgacggc agccagcagc gggtctcgcg gaagcatgac
     4261 ggccacgtag accgccagcg tcagcaaact caccgtagta gtcgcgagat cgcccgaaat
     4321 cagtcgagcg actcgcgccg tactcgcagc cctgctgacg acgtcgccgg tccagcgatg
     4381 ctgaaaaaac gccagaggca gccgcaagac gtgccacatg aattcgatcg attcgaccag
     4441 cgtcagccgg acttccaggc gggtcaggta gacctgttcc agcgcggccg ccgcgagtcg
     4501 aaacgccgcc gtcaaggcca tggcccacag cagcggcttg agccattcgt ggtgctcttc
     4561 gatcaggatt tcatccaggt aaacgcgctg aaacgcggcc gagatcaggt tgggaatgac
     4621 gagtgccaag ccagccagga tcacaaagac gagcgctgtc ttggatgtcg acagccgccg
     4681 caccaggccg agaaccgcgc tgggccgctt cccctttttt tggaactgtg ggccgagctc
     4741 gaaggtgaac gcgatgccgc tgtagccgcg aacgaattcg tcaaatccaa tcgagcgccg
     4801 gccacttgcg ggatcgttca gatacacctt gccgcgccgg aacccctcga ctaccaggaa
     4861 gtgattccat tcccagaaga caacgaacgg tggccggcgg ttcgatagcc cgtcagccgg
     4921 cttgcgaaat gcattgactt cgagtccgta gcgccgcgct gcttccttga tgtagaacgc
     4981 gttgctccca tcgcgcgaga cgccgcagtc atcgcgcagc acgtcgaggg gaacatagcg
     5041 gccgtgatac tccagaatga tccccagcgc ggcggcgcca cattcagccg cctccatctg
     5101 gaggatggtc ggtgtgcgga ccgggcggta cttgcgagat gccgcgctag ccatgcctta
     5161 ccgcctcccg caatagcccg gcttccgggt taccgcctac cgcatttctg ggtcttgtcc
     5221 gtcgcggatg ctcgccggac tagatcccga tatcaattat gcacgagcgc ggcaggccac
     5281 gccgcgagcc gtcccttcaa accaacggga attccgcgac cgatctattc ggcccccacg
     5341 actctcttgg tccatgggag tatcagactg atcagttgcc gtttctcgac cacaatcgcg
     5401 gccttgcaca tcgtcccggt cttgagcgac tgggcgcgtc ccgacgacga cgaccattcg
     5461 aatcggttgc cacgcacggg ttgtagcgga ctcgccaccg acgccgaggc ggtagctcgc
     5521 tcgttgagct tgacatccac acggagcaac acgcccggcg cgtaccgcct gagaaacgcg
     5581 tctacaagct gcggatgctc gagcgtggct tccatagcca ggcgggtggc cggcagctcc
     5641 gagatagcaa ctaccttgcc tttgataaag ccgtgctctt cacgcttgac ggtcgtcggg
     5701 ctcacctcga catcatcgcc aagaccgatc ttcttccctt cggccgcggg tacgaatagg
     5761 attgaatcgt acagcggacc ggcctcggcg attccgagat ctaccctggg tgtatgaagc
     5821 aggacgaccg gcgacccctc gtggaccagc tcgccccggg cgctcaaaac ctccgcgacg
     5881 tgaccgtgca ccttgctgac gacgcgcgaa gtacgactca gtttgtcgca atcgagcgcc
     5941 agcttggtct ttaactgcct gatcttgagc cgtcgttcga gctgggcccg cttccgcgcg
     6001 tagtcggtgg tggtcgcttc caggtccagc tccgccagcc gcgattggcc cttgttcaag
     6061 tcatccttga tcacatagag tttttcccgc gattccagca gctccatatc gcccagatat
     6121 ttctgagctc ggagccggtc ggcgc
//
LOCUS       DATAQL010001220         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3636861, whole genome shotgun sequence.
ACCESSION   DATAQL010001220 DATAQL010000000
VERSION     DATAQL010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3636861"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VHS97_21040"
     CDS             <1..205
                     /locus_tag="VHS97_21040"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3450754.1"
                     /translation="AMTQEFLAFMLGVRRQTVTVVAGTLQNAGLIRFRRGVMVILDRP
                     RLEEAACECYATIRDYYEQYVVV"
     gene            474..1535
                     /locus_tag="VHS97_21045"
     CDS             474..1535
                     /locus_tag="VHS97_21045"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450755.1"
                     /translation="MTIPTEPIGSIPRPLRLIESIANVGGEDPSLAPLFDEAIRDTIK
                     RFEATGSPVITDGEQRKYRGFWTYSVHGLDNMAPDGFKIPFSAGHTRRMPRITSGPFR
                     YRTYADSYLDVALRYAHVPVKQAVISPSAMSLLYPAEQIAQYPRERFIDDLLNEHETE
                     VRNCLQKGAHKVQIDFTEGRLAIKIDPSGALLHSFIDLNNLALSRFSASERERIGVHT
                     CAGSDCDSTHSVDVDYAELLPSLFQLKAGSFYIALAGEKDRVRVLKIIREYLKPHQRA
                     FVGVVSPIDPRVETPEEVRDRIVEAAQFIPLTQLGTTDDCGFSPFFDDTSTSRDTAFA
                     KIRSRVLGTALASEFLGGD"
     gene            1535..>3116
                     /locus_tag="VHS97_21050"
     CDS             1535..>3116
                     /locus_tag="VHS97_21050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3450756.1"
                     /translation="MRAGDDEEELLRSVALQNSRSILLARQRAEGALAQQSEWLRITL
                     ASIGDAVICTDAEGRVTFLNGVAENLTGWSQADALGRPLPEVFQIINEVTRQPVENPA
                     LRALRENAIVGLANHTILVASDGTERPIDDSAAPMRNGDGVVVGTVLVFRDVTERKRA
                     EETRALLAAIVESSEDAIVSKTLDGTIRSWNSGAERLFGYTRDEAVGRSIKLIVPPEL
                     LDEESRILERLERGERIDTLETVRVTKQNRRVEVSLTISPVRDSSGRVIGASKIARDN
                     SERKRVEEALREADRRKDEFLALLAHELRNPLAPLRNGLEVMRLATGDPSAVAQAREM
                     MVRQVGHMVRLIDDLLDISRINRGKMELRCSRVLLVDVINSAVETVRPLIDAAGHRLE
                     ISLPPEHVILDADLTRLAQVFGNLLSNSCRYTERGGHIWLAALRRGDVVSVTVRDNGI
                     GIPAESLPRIFDMFSQVDRSIERSTGGLGIGLALVKGLVEMHGGTVTAESAGPKKGTT
                     FTVELPVLASYTTPATLEN"
BASE COUNT          620 a          944 c          993 g          559 t
ORIGIN      
        1 cgcaatgact caggagtttt tggctttcat gctgggcgtg cgccgccaga ctgtgacggt
       61 cgttgccggc actctacaaa acgctggcct gatccggttt cgcaggggtg taatggtcat
      121 tcttgaccgg cctcgtctcg aggaggccgc ctgcgagtgc tacgccacga tccgggatta
      181 ctatgagcag tacgtcgtcg tctgaaatcc cgatcgccgg gaacgcgacg tcgaaaggat
      241 gatcgtgatg aaaaaccaaa aacccaaaac cattacttag atatgtccgc cagcggacag
      301 acgggatctg ggaattcacg tagaataaag gacaatagct ggcggctggc ccgtgcgtat
      361 gcgcgcgcag cgagacgcgc ccacggcgcc gctggagtat cgtcgtgcga ctgagaggct
      421 aagcggaggc gcggtgtcgc gccagacccg cgcgggtcga ggcgaacttg catatgacga
      481 tcccaacgga accgatcggg agtattccaa ggcctctgcg gctgatcgag tcgatcgcca
      541 acgtcggtgg cgaggaccca agcttggccc cgctctttga cgaggcgatc cgggatacga
      601 tcaaacggtt cgaagcaacc ggctcgccgg taatcaccga cggcgagcag aggaagtacc
      661 gcggtttttg gacgtatagc gtgcatggcc tcgacaatat ggcaccggac ggcttcaaaa
      721 ttccgttttc agccggtcac actcggcgga tgccgcggat cacgtccggc cctttccgct
      781 accggacgta tgcggacagt tatctggacg tggctctgcg ctatgcccac gtgccggtga
      841 aacaggccgt catctcccct tcggcgatga gcctcctgta cccggccgag cagatcgccc
      901 aatacccgcg cgaacggttc atcgacgact tgctcaacga gcatgaaacc gaagtccgca
      961 attgtctgca gaaaggggcg cataaagtcc agatcgattt caccgagggg cggttggcga
     1021 tcaaaatcga cccttccggg gccctgctgc acagcttcat cgatcttaac aatctggcac
     1081 tatcgcgttt ttctgcgtcg gagcgcgagc gcatcggagt gcacacgtgt gcgggaagcg
     1141 actgcgattc cacgcacagc gtcgacgtcg attatgcgga actgctgccc agcctgtttc
     1201 agctcaaggc ggggagtttc tacatcgcct tggcgggtga aaaggaccgc gtccgagtcc
     1261 tgaagatcat ccgcgagtac ctcaagcccc accagcgggc tttcgtcggc gtcgtttcgc
     1321 cgatcgaccc gcgagtggag acgccggagg aagtccggga ccgcattgtt gaggcggccc
     1381 aatttatccc gctcacccag ctgggaacga cagacgactg cggcttttcc ccgttctttg
     1441 atgatacctc gacaagccgc gacacggcct tcgcaaagat tcgctcgcgt gtgcttggaa
     1501 ccgcgctggc atcggaattc ctcggagggg attgatgaga gcgggagacg acgaggaaga
     1561 actgctccgt tccgttgcac tccagaactc cagaagcatc ctgcttgcgc gccagcgggc
     1621 cgaaggggcg ctcgcgcagc agtcggagtg gctgcgaatc acgctcgcga gcatcggcga
     1681 cgcagtgatc tgcaccgacg cagagggccg cgtgacgttt ctgaacggcg tggccgagaa
     1741 cttgaccggc tggtcccagg ccgacgcgct gggacggcct ctgcccgaag tcttccagat
     1801 catcaacgaa gtgacccgcc agccggtcga gaaccccgca ctgcgggctt tgcgagaaaa
     1861 cgccattgtt gggctggcga accacacgat tctcgtcgcc tcggacggca cggaacggcc
     1921 aatcgacgac agcgctgcac ccatgcgaaa cggggacgga gtcgttgtgg ggacagttct
     1981 ggtcttccgg gatgtcacgg aacgcaagcg ggccgaggag acccgagctc tcttggcggc
     2041 gatcgtcgag tcgtccgaag acgcgatcgt cagcaagacg ctcgacggca ccatccgctc
     2101 gtggaattcc ggggccgagc ggctctttgg ctacacgcga gacgaggccg tcggccggtc
     2161 gatcaagctg atcgtcccgc cggaacttct ggacgaggag tccaggatcc tggagaggct
     2221 agagcgcggc gaacggatcg acactctcga gaccgtccgg gtcacgaagc aaaaccgccg
     2281 agtcgaagta tcattgacca tctctccggt ccgagacagt agcggccggg tcatcggcgc
     2341 gtccaagatc gctcgcgaca actccgagcg gaagcgggtg gaggaggcgc tgcgcgaagc
     2401 ggaccgccgc aaggacgagt tccttgcgct cctcgcgcac gagctgcgta atccgctggc
     2461 accgctgcgc aacggcctgg aagtaatgcg gctggcgacc ggcgacccga gcgccgtcgc
     2521 tcaggcccgc gaaatgatgg tgcgccaggt cggccacatg gtccgtctga tcgacgacct
     2581 gctcgacatc tcgcgcatca accgcggcaa gatggaactg cgctgctcgc gggtgttgct
     2641 ggtggatgtg ataaatagcg ccgtggaaac tgtccgcccg cttatagacg ccgcgggtca
     2701 caggctcgag atctctctgc cgccagagca cgtgatcctg gacgctgatc taacccggtt
     2761 ggcccaggtc ttcggcaact tgctttccaa cagctgccgg tacactgagc gtggtgggca
     2821 tatctggctg gccgcactgc gtcggggcga cgtcgtgtca gtcaccgttc gcgataacgg
     2881 catcggcatc ccggccgagt cactgccccg tatcttcgac atgttctcgc aggtggatcg
     2941 gagtatcgaa cgatccacgg gcgggcttgg catcggtttg gcactggtga aagggctggt
     3001 ggaaatgcac ggcgggacgg tcacggccga gagcgccggg ccgaaaaagg gaaccacctt
     3061 caccgtcgag cttcccgtct tggcgagcta caccacgccg gcgactctgg agaatt
//
LOCUS       DATAQL010001221         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_428633, whole genome shotgun sequence.
ACCESSION   DATAQL010001221 DATAQL010000000
VERSION     DATAQL010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428633"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1143
                     /locus_tag="VHS97_21055"
     CDS             223..1143
                     /locus_tag="VHS97_21055"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEX3450757.1"
                     /translation="MIAPSGEQITILAKDQQAVVVAVGGGLRSYSVGGRELLDGYSAD
                     EMSSSGRGQVLIPWPNRLQDGSYEFNGRRHQLPLNEPEHCNAIHGLVRWSTWTATERQ
                     PHRVVMEHILYPQPGYPFLLRISIEYALSDSGLLVQTTATNLGARTCPYGGGAHPYLT
                     LGTATIDGLILQVPGRSVLRSDERGLPIRKEAVEGTEYDFRQPKQIGSIKLDHAFADL
                     ERDADGLARVELRDSDRETKVSLWVDQSYPYLMLFSGDPLPNVHRRSLAVEPMTCPPN
                     AFRTGDALIRLEPGASFTGTWGIARGSAAS"
     gene            complement(1267..1485)
                     /locus_tag="VHS97_21060"
     CDS             complement(1267..1485)
                     /locus_tag="VHS97_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450758.1"
                     /translation="MFRLQGGEWVTREIVRDVQSGRAKFVVNSGDAVWWGNQGLTVSD
                     SPYWKRVNDTMLKKLPAPDDEMRAAGLA"
     gene            1567..1750
                     /locus_tag="VHS97_21065"
                     /pseudo
     CDS             1567..1750
                     /locus_tag="VHS97_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607949.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          369 a          546 c          492 g          343 t
ORIGIN      
        1 cgttgagttg atgacgaagc gtcaaaatct ctgcttgcag cgacgctcgc gaccggaaca
       61 atccgatcag cgccaaccaa atcaggctgc aaacatctct catcaaccaa gagcatcacc
      121 caattcgact ccacttgcta ggcggattga gtttccgaca atgacaggcc ttgctcgatc
      181 tccgcacccg gctatagttc agtcaccagt ggaggcgaag ggatgatcgc accatccggc
      241 gagcagatca cgatcctagc caaagatcag caggctgtag tcgtcgcggt tggcggtgga
      301 cttcggtcat actcggttgg cggccgtgag cttctcgacg gatatagcgc cgatgagatg
      361 agttcctcgg gccggggcca ggtgctgatc ccttggccga acaggttgca agatgggagc
      421 tatgagttca acgggcgtcg acaccagctc ccactgaacg agccggagca ctgcaacgcg
      481 atccacggtc tcgttcgttg gtccacctgg accgccaccg agcgacagcc acatcgtgtg
      541 gtgatggaac acattcttta tcctcagccg ggctacccgt ttttgctcag aattagcatc
      601 gaatatgcac tatccgacag tggactcctg gtgcaaacca cggccacgaa cctcggcgcg
      661 cgcacctgtc cttacggagg cggcgctcat ccatatctga cccttgggac cgcaactatc
      721 gacggtttga ttttgcaggt acctgggcga tcggtcctgc ggtctgacga gcgcggcctt
      781 ccgatccgga aggaagccgt ggagggaacc gaatacgatt ttcggcaacc gaaacagatc
      841 ggctcgataa agctcgacca cgcatttgca gaccttgagc gggatgcgga tggcctcgct
      901 cgtgtggagc tccgcgattc ggaccgcgaa acaaaagtct ccctttgggt cgaccaaagc
      961 tacccgtact tgatgctctt cagcggcgac ccacttccaa acgttcaccg tcgcagcctt
     1021 gccgtcgagc ccatgacttg cccgccgaac gcatttcgaa ccggcgacgc tctgatccgc
     1081 ctggagccag gcgcctcgtt cactggtaca tggggcatcg cacggggaag cgcagcctcg
     1141 taaccggcgc ggtctcaagg gccgagaccg tgtgtccaaa cgcgcccaag cttccctccg
     1201 gaatctcgat tacaaagcaa tagaggcctt tcaaataggc aggagcgatg tgttggccac
     1261 cccaatctaa gctagacccg ctgcgcgcat ctcgtcgtct ggcgcaggca gcttcttcaa
     1321 catcgtatcg ttcacccgct tccagtacgg gctgtcgctg acggttaagc cctgattgcc
     1381 ccaccaaacg gcgtcgccgc tattgaccac gaatttggcg cgaccggatt gaacgtctct
     1441 cacaatttca cgggtcaccc actcgccgcc ctgcagccga aacattgtcc gatgcacccc
     1501 cggaagcaat ttaagcagcg gtcgatcgcg ggtcaggact ggcacaagga ccgcgatccg
     1561 aagagcgcca gccgtgcggt cagggagtat ctggcgaccc tggatgatac ggcgtggggc
     1621 accgccagcg acgtcgtccc gaagtttgtc tcgccatccg atccggcagc ccagtggacc
     1681 ggggctcaca aggggccggc attcttcgct tactcggaca attatctgat cgacgtgaag
     1741 ttcggcgtca
//
LOCUS       DATAQL010001222         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3177161, whole genome shotgun sequence.
ACCESSION   DATAQL010001222 DATAQL010000000
VERSION     DATAQL010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3177161"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1425
                     /locus_tag="VHS97_21070"
     CDS             121..1425
                     /locus_tag="VHS97_21070"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX3450759.1"
                     /translation="MVREDTSGPDPTPRSVGGRSGRPAVIVGLSLAALGGAACWLVFS
                     ATRPQTVIQRTGAVQLLTVDRPFPAEGRFPGDRYAGTQVCAECHPAEAALHFRSGHSS
                     TLLPAGRRALARRLDGKTVTDPERPEVAWSYQFRDGHLRIARTADGQSQKWVVDYAFG
                     SGHHATTFVNVLDASIPSIFEHRLTYFTQEAALAITPGQDVLSRVAEFTPDGNKFTTK
                     QARKCIRCHSTQTSARGDEMLDEDTMIPNVSCERCHGPSRGHVLAARRGAGEPDLSLP
                     FGPDRFTAESLLKLCGACHRHPSSARPGQIRPDNPFLARFQPVGLMQSKCYARSEGAL
                     SCVTCHDPHARSSPDRVSYNAVCLECHGAHGPTTMPAADIHSRVVCPVSPRERCVECH
                     MPKVDSGQHILFSDHWIRVRRQGESSLSIPTSVPGLDLLAPD"
     gene            complement(1814..>2345)
                     /locus_tag="VHS97_21075"
     CDS             complement(1814..>2345)
                     /locus_tag="VHS97_21075"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX3450760.1"
                     /translation="IPNRMFKNVGGERFAEITASSGTGHLQKGHSVSCADWDRDGDID
                     VFIEMGGAVNGDKSHNILFQNPGQSNDWLTVKLIGQKSNRPAIGARIKVVTAGERPLT
                     IHRHVSSGSSWGANPLQQTIGLAKADRVALLEIHWPTSGTTQVFRDIAVNQAIEVTEF
                     ANDYQRLDWKPIPVTK"
BASE COUNT          411 a          731 c          737 g          466 t
ORIGIN      
        1 gcccgcggtt tgccgcgata gaattcgggc cttaacattg ccgccaggcg tgaaccgccg
       61 ccggggagcg tcggtacgat cgggcgcgcg ggttgctgag attcaagatt ggcttgatcg
      121 atggtacgcg aagatacctc cgggcccgat ccaacgcccc gttcagtcgg aggcaggtcg
      181 ggtcggccgg cggtgatcgt tggcttgagc ctggcagccc ttggcggggc cgcatgttgg
      241 ttggtctttt cggcgacccg cccccagaca gtgatccagc gcacaggcgc ggtccagctc
      301 cttaccgtgg accggccatt cccggcggaa ggacgatttc ccggcgaccg ctatgccggc
      361 acgcaggtct gcgccgagtg ccatccggca gaggccgcgc tccatttccg atcggggcac
      421 tcctcgaccc tgttaccggc gggcaggcgc gccttggctc gccggctgga cgggaagacg
      481 gtcaccgatc ccgaacgccc tgaggtcgcc tggagctatc agttccgcga cgggcacctg
      541 cggatcgcgc gcacggcgga cggccagtcc caaaagtggg tggtcgatta cgcgttcggc
      601 tcgggccatc acgccacaac gttcgtgaac gtgctcgatg cctcgatccc gtcgatattc
      661 gaacatcggc tcacctattt cacgcaggag gctgcgctgg cgatcacacc aggccaggac
      721 gttctatcgc gggtcgcgga attcacgcct gacggcaata aattcacgac gaaacaggcc
      781 cgcaagtgca tacgctgcca ctcgacacaa acttctgccc gtggcgacga aatgcttgat
      841 gaggacacga tgatccccaa cgtttcgtgc gagcgctgtc atggcccgag ccgaggccat
      901 gtcctggcgg cgcgtcgagg tgcgggggag ccagatctgt cgctgccgtt cggcccagac
      961 cgttttaccg ccgaatcact cctgaagttg tgcggcgcat gtcaccgtca cccatccagc
     1021 gcccgaccgg gccagatccg tcccgacaac ccgtttctgg cgaggtttca accggtcggg
     1081 ctgatgcagt cgaagtgcta cgctcggagc gagggggctt tgagttgcgt cacgtgccac
     1141 gacccccatg cgcgctcttc acctgatcgt gtgtcgtaca atgcggtctg tctcgagtgc
     1201 catggcgctc acggtcccac cacaatgccg gcggccgaca ttcattcccg tgtcgtttgc
     1261 ccggtgtcgc cgcgggagcg gtgtgtggaa tgtcacatgc cgaaggtcga ttcaggccaa
     1321 cacatcctct ttagcgatca ctggatccgg gttcggaggc agggcgaatc ctcattgtcg
     1381 attcccacct ccgtgcccgg cctcgactta ctcgctccgg attagccctg accgaaacgg
     1441 gcttgtccat cggcggctcg tgtcgctcat tatgcgtcga cgagcgtagg aaagcatcgt
     1501 gccacatacg gagggtcgag tcgccggagc tgaccggtcc caatccatcc gatccgaatc
     1561 aaggccggtc gccggccaaa cggggtccac tggtctccct ggccgagggc gattttgtgt
     1621 acatccgcgg tgaaggtgat ggggctgagg aattatttga cgaacgcaac gatcccgacg
     1681 agttacgcaa tcgggcgggg atcgaggcat tgcaacccgt cctgaagcgc tttcgcgaac
     1741 gtgtgaacca attgaaatcg agacctcgat tccgatggca cgagggtgac ggtaaagttg
     1801 cctgaacgct cgttcatttc gtcaccggga ttggtttcca gtcgagcctc tggtagtcgt
     1861 tcgcgaactc cgttacctcg atggcttggt tcacggcaat gtcccgaaac acctgggtcg
     1921 tgccgctcgt cggccaatgg atctccagga gcgcaacccg gtcggccttg gccaggccga
     1981 tggtttgctg gagtggattt gctccccaac tgctgcccga ggaaacgtgc cggtggatgg
     2041 tcaaggggcg ctcgccagcc gtgaccacct tgatccgggc gccgatggcc ggccggttgg
     2101 acttttggcc aatcagcttg acggtcagcc agtcgttgct ttgaccggga ttctggaaaa
     2161 gaatattgtg ggatttgtca ccgttgacgg ccccgcccat ctcgatgaaa acgtcgatgt
     2221 cgccgtcgcg gtcccagtcg gcgcacgaca cagaatgccc cttctgaagg tggccggtgc
     2281 ccgacgacgc ggtgatctca gcgaatcgct cgccgccgac attcttgaac atgcggttag
     2341 ggatc
//
LOCUS       DATAQL010001223         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1804832, whole genome shotgun sequence.
ACCESSION   DATAQL010001223 DATAQL010000000
VERSION     DATAQL010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804832"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VHS97_21080"
     CDS             complement(<1..293)
                     /locus_tag="VHS97_21080"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3450761.1"
                     /translation="MNESARNLVLGTAGHIDHGKTALVRALTGVDTDRLPAEKERGIT
                     IDIGFASLDLDGIHLALIDVPGHERFIRNMLAGASGLDLAMLVVAADDSVMPQT"
     gene            423..908
                     /locus_tag="VHS97_21085"
     CDS             423..908
                     /locus_tag="VHS97_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450762.1"
                     /translation="MLLTFLIQVSRAGFVTDRGNAPPFTQFSVVPNNYRCRPWPDRHF
                     SLFVTIIKVTMARKRPYSLMFDVEVKQHLRAIEAKYLSLIRATIEEQLSFEPENETRN
                     RKPLERPTELGATWELRFGPNNCFRVFYTVVAEHWEVQILAIGIKERNRLIIGGEELE
                     I"
     gene            905..1201
                     /locus_tag="VHS97_21090"
     CDS             905..1201
                     /locus_tag="VHS97_21090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX3450763.1"
                     /translation="MKFASVADVKAHFSAYLKASEQGPVVVTRNGKPAAVILAVSDDE
                     ELERLMMAHSPRLRAILEAARRRIQAGAGVRDEEFWQDLEGAEEPKKRGRKLAK"
     gene            1267..1668
                     /locus_tag="VHS97_21095"
     CDS             1267..1668
                     /locus_tag="VHS97_21095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3450764.1"
                     /translation="MGQAIAHVAIVVRDYDEAIAYYTNALGFDLVEDTPLGAGKRWVL
                     VRPPGSDGTCLLLARAASPEQESRIGNQTGGRVFLFLHTDDFWRDYNAMKTRGVQFIQ
                     EPREEAYGTVVVFVDLFGNKWDLVERKSRGS"
     gene            1658..3091
                     /gene="selA"
                     /locus_tag="VHS97_21100"
     CDS             1658..3091
                     /gene="selA"
                     /locus_tag="VHS97_21100"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX3450765.1"
                     /translation="MDRDALRQLPPVGTVLEHALLEPCKRARGRAAVLRAVRGAIAEA
                     RAAIQLGAAAAVDPESLARRSQQLLDSERASLRPVINATGILLHTGLGRAPLAEEAIA
                     AVSDVARGYCNLELDLEEGSRGRRTEGVARLLCEATGAQAATAVNNNAGATILALKAL
                     AAGREVIVSRGQLVEIGGSFRLPEILEVSGAILHEVGTTNKTRLSDFERAIGPRTAAI
                     LRVHPSNFRIVGFTEEPELAELVALAHDRGLALIDDIGSGALGPGRPPGVCDEPTAAA
                     GIAAGADLVLFSGDKLLGGPQCGIIAGTAQAVAQVERDPLMRALRLDKMTLAALEATL
                     LLACDADRAASRIPLWSMIGAPVAALSERATRLAVVFRDELGLNAAAVAAESYLGGGS
                     APVQPIPTAAVVVSPPFPIHLGSEAALALALRRGDPPVVTRVQKGLVVFDLRTITEDR
                     LPVLLDSVRKVCHDRNSQATSDGPPYG"
     gene            3171..>3629
                     /locus_tag="VHS97_21105"
     CDS             3171..>3629
                     /locus_tag="VHS97_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HEX3450766.1"
                     /translation="MRVCRFGFDEMVLTGFYADDQVIPIDQAAEAYSRDMGVELLLPS
                     TEDLLDLLPPDGSSYEAASELFAWIEQLDLISRDELTIPTSDVRLLVPIANPPKMLFL
                     AGNYAKHVAERGGSAAERQETFPYVFMKPPSTTLTHPGDPVLIPRVSPDQI"
BASE COUNT          685 a         1094 c         1155 g          695 t
ORIGIN      
        1 gtctggggca tgaccgagtc atcggccgcg acgacgagca tggccaggtc cagccccgac
       61 gcgccggcga gcatgttgcg gatgaaccgt tcgtggcccg gcacgtcgat gagagcgagg
      121 tggatcccgt cgagatcgag cgacgcgaag ccaatgtcga tcgtgatccc acgctctttt
      181 tcggcgggca gccggtcggt atccacgccg gtcaatgcgc gaaccagggc cgtcttgccg
      241 tggtcgatgt gtccggcggt tcccagcacc aggttgcggg cgctctcgtt catggtcttg
      301 gtatcggtgg gttgtcttgg cccgtcgctt cctttgtcga ttccactcgg ctagtttagc
      361 ggccaagacg cttgacgagc gagtggtagg gcaagcgctg acagtggtcg gcggaattcg
      421 aattgctatt gacttttttg atccaagtgt cgcgcgcggg tttcgtgacg gatcggggca
      481 acgcaccgcc gtttactcag ttttcggttg ttccgaacaa ctatcgctgt cgaccttggc
      541 cagatagaca tttttcgctc ttcgtgacca taattaaggt cacaatggca cgtaagcggc
      601 cttactctct gatgtttgat gtggaagtca aacagcatct tcgcgctatt gaggccaagt
      661 acctctcact tattcgagcg acgatcgagg aacaactatc cttcgaaccc gagaacgaaa
      721 cacgaaaccg caagccgttg gagcgtccca cagaattggg ggcaacgtgg gagctcaggt
      781 ttggaccgaa caactgtttt cgcgtgttct atacggtcgt cgcggagcat tgggaagtac
      841 agatcctggc catcggaatc aaggaacgga atcggctcat catcggtgga gaggagttgg
      901 agatatgaag ttcgcctccg tggctgacgt aaaagcccac ttcagcgctt acctgaaagc
      961 cagcgagcaa ggtccggtcg tcgtcacccg gaatggtaag cccgccgcgg ttatactagc
     1021 ggtcagtgat gacgaagagc ttgaacgtct gatgatggcc cactctccga ggcttcgagc
     1081 gattctcgag gccgcccgac gacggatcca agccggtgct ggtgttcgcg acgaagaatt
     1141 ctggcaggac ttggaaggcg ccgaggaacc gaaaaaacgt ggtcgaaaac tcgcgaaata
     1201 atgagagccg tgacggaaat gacgccgaac gaactgaatg cgaaaaatgc ggagacctga
     1261 cgaataatgg gccaagccat cgctcatgtc gccatcgtgg tccgagacta cgacgaggcc
     1321 atcgcctact acacgaatgc cctcggattt gacctggtcg aggacacgcc gttgggcgcg
     1381 ggcaagcgat gggtcctggt gcgcccgccg ggctcggatg gcacctgcct gctgctcgcc
     1441 agggccgcca gcccggaaca ggaaagccgc atcggcaacc agaccggcgg ccgtgtgttc
     1501 ttgttcctgc ataccgacga cttctggcgc gactataacg ccatgaagac gcgcggagtc
     1561 cagttcatcc aagagccccg tgaagaagcg tatgggactg tggtcgtctt tgtcgatctc
     1621 tttggcaata aatgggacct ggtggagcga aagtctcgtg gatcgtgatg cgctccgcca
     1681 gttgcctccg gtcggaacgg tgctggagca tgcgctgctc gagccgtgta agagagcgcg
     1741 cggccgcgca gccgtgctgc gggcggtccg tggcgcgatc gcggaagcac gcgctgccat
     1801 ccagttaggg gcggccgctg cggtcgatcc cgagagtctg gcgcgtcggt cgcaacaatt
     1861 gctcgacagc gagcgcgcta gtctgcggcc cgtgatcaat gcgacgggaa tcttgctcca
     1921 taccgggctg ggccgtgcgc cgctggcaga ggaagccatc gcggccgtgt ctgacgtcgc
     1981 gcgcggctat tgcaatctcg agctcgacct ggaagagggc tcgcgggggc gccgtaccga
     2041 gggcgtcgcg cgcttgctgt gcgaggcgac aggggcccaa gccgcaacgg ccgtcaacaa
     2101 caacgccggg gcaacgatac tggccttgaa ggccctggcg gcggggcgcg aggtgatcgt
     2161 ctcgcgtggg cagttggtcg agatcggcgg cagctttcgg ctccccgaga tcttggaagt
     2221 ctccggagcc atccttcacg aggtaggcac aaccaacaag acccggcttt cggactttga
     2281 gcgcgccatc ggcccgcgga cggcggcgat cttgcgcgta caccccagca attttcgaat
     2341 cgtgggcttc accgaggaac ccgagctcgc cgagttggtt gccctggccc atgaccgcgg
     2401 gctcgccttg atcgatgata tcggctcggg agcgcttggg ccgggtcggc cccccggcgt
     2461 ctgcgacgag cctacggccg cagcgggaat cgccgccggc gccgatctgg tgctcttctc
     2521 gggcgataag ttattgggag gtccccaatg cggcatcatc gccgggaccg cccaggcggt
     2581 cgcccaggtc gagcgcgacc ccttgatgcg tgccctacgc ctggacaaga tgacgctggc
     2641 agcgctcgaa gccacattgc tcttggcatg cgacgccgat cgagcagcca gccggatccc
     2701 tctctggtcg atgattgggg ccccagttgc ggctctgtca gagcgtgcca caagactggc
     2761 ggtcgttttc cgcgatgagc tggggctcaa tgccgcggcg gtggcggcgg agtcatacct
     2821 ggggggcggc agcgcaccgg ttcagcccat accgaccgcg gccgtcgtgg tttcaccgcc
     2881 gtttccgatt caccttggat cagaagcagc cctggcgctg gctctgcggc gaggcgaccc
     2941 gcccgtcgtg acacgcgttc aaaagggact tgtcgttttt gacttgagga caattaccga
     3001 ggatcgcctg ccggttctgc tcgactccgt ccgaaaagtc tgtcatgatc ggaactcaca
     3061 ggcgacgtcg gacggaccgc cctacggttg acctcggccg ccaaccgaga tgaggtttga
     3121 gccagtcacc gaacaggaaa tagaacaccg cgaatttaag gagagccggg atgcgtgtct
     3181 gccggttcgg ttttgatgag atggtgctga cgggatttta cgcggacgac caggtaatcc
     3241 ccatcgatca ggccgccgaa gcctacagcc gcgatatggg cgtcgagctc ctgctcccct
     3301 cgacggaaga cctcctcgac ctgctccctc ccgacggcag ctcttatgaa gccgccagcg
     3361 agctcttcgc ctggatcgaa cagcttgatc tgatctcacg cgacgagttg acgatcccca
     3421 cctccgacgt ccggctcttg gtgccaatcg ccaatccacc caagatgctc ttcttggccg
     3481 gcaattacgc caagcacgtc gccgagcgcg gcggatcggc ggccgaacga caagagacgt
     3541 ttccgtatgt ctttatgaag cctcccagca cgacgctgac gcaccccggc gacccggtct
     3601 tgatcccccg cgtctcgccc gaccagatc
//
LOCUS       DATAQL010001224         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2872153, whole genome shotgun sequence.
ACCESSION   DATAQL010001224 DATAQL010000000
VERSION     DATAQL010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872153"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="ltrA"
                     /locus_tag="VHS97_21110"
     CDS             complement(<1..896)
                     /gene="ltrA"
                     /locus_tag="VHS97_21110"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX3450767.1"
                     /translation="MKKIKVHSLTGRITSRLMRQAFQAVKRNRGAAGVDKVSIQLFAA
                     NLEENLAALMRELKTGTFRPLPLRRVLIPKGPGSTKLRPLGIPVVRDRVAQEVIRRLL
                     APIFEPQFHENSYGFIPGRNCHQAIERVLKLHEDGFRVVLDADIQGFFDNLSHRVIMA
                     AVAAEVADGNILTLVERFLTSGVMEDGVFKPTTIGTPQGGVVSPLLANIVLNRLDWQL
                     HEAGYRFARYADDFVVLCQDPQEAQEALNLVKHVLENDLGLQLSPEKTKTTTSGKGYD
                     FLGFHLMSRSRRMRDKSVQKFKA"
BASE COUNT          265 a          487 c          488 g          349 t
ORIGIN      
        1 gccttgaact tctgcaccga tttgtcgcgc atgcgtcggg aacgagacat gagatgaaac
       61 cccaggaagt catacccttt cccagaggta gtcgtcttcg tcttctcggg actcagttga
      121 agtccgagat cgttttccaa cacgtgcttg accaggttca gtgcctcttg cgcctcctga
      181 gggtcctggc agagcaccac gaagtcgtcg gcataccgcg cgaagcgata gccggcttcg
      241 tggagttgcc agtccagacg attcagcacg atattcgcca gcaacggcga aacgacgcct
      301 ccttgcggtg tgccgatcgt cgtgggcttg aagactccgt cttccatcac ccccgaggta
      361 agaaatcgtt cgaccagggt caggatgttc ccgtcggcca cctcggccgc cacggctgcc
      421 atgatcacgc gatgcgagag attgtcgaag aacccttgga tgtccgcgtc gagaacgact
      481 cggaacccgt cctcgtggag cttcagcacg cgctcgatcg cctggtggca gttgcggccg
      541 gggatgaatc cgtacgagtt ctcgtgaaac tgtggttcaa agatcggagc aagaagcctt
      601 cgaatcactt cctgcgcgac ccgatctcgc accacgggaa tgccgagcgg tcgaagcttc
      661 gtcgaaccag ggcctttggg gatgaggaca cggcggagcg gcaggggccg gaacgttccc
      721 gttttcagct cccgcatcag cgccgcgagg ttttcctcga ggttggcagc aaacagttga
      781 atgctgacct tgtccacgcc cgcggccccg cgattccgct tgacggcttg gaaggcttgt
      841 cgcatgagcc gggaggtaat cctgccggtc agcgagtgca ccttgatctt tttcatggcg
      901 ggcctttcac gcgttgcaat cacggagttc ggatcagcga gcttttgggg acgtttctta
      961 gcgtcgccgc ggttacggct ttccctgggt ttcccggcaa gagcgcgggc gggccatttt
     1021 gtggtttgag ccagggggct aactctcctc gtggtctcgc cggtcgttcg ccgtcttcag
     1081 tcggtttctc ctttcacttc gcgcctgggc gcgaagctac tccggatctg ctgctgccct
     1141 tgaagaggag gcggtttagg cgaccttctc ctcaccaccg cgtagccggc ccctttcgcc
     1201 aatggcttat aggaacggct taccgaccgt cggacggtgg agggcttcac cggtcatggc
     1261 ttgggaccat tttctcccgg cggcggcacg tagcacccaa ttggtccgca cgcggcaatc
     1321 cgcatcaccg tgtgtggggc gagaggttgg gtctctcgtc agcgcttcgt cgaagggcgg
     1381 tgagtggcgc accaactcgg ccgaaaatgc cgtctcccgg agtcaccttg cctggcccct
     1441 tcgtcgcgcc gccttctccg gacgacccgg tggcgacgcg ctggctgcgt acctccgcgg
     1501 aaattgcgga gttcgagcaa ggcgttctgt acgggtgcgg tcccgcacag gtaggctttg
     1561 gcggtcaatt atcctccttt catcgcctc
//
LOCUS       DATAQL010001225         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4396818, whole genome shotgun sequence.
ACCESSION   DATAQL010001225 DATAQL010000000
VERSION     DATAQL010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4396818"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VHS97_21115"
     CDS             <1..270
                     /locus_tag="VHS97_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3450768.1"
                     /translation="RGVLIDISSLDMVDSFIGRMIGNIAGMSRVLDAKTVVVGMRPAV
                     AITLVELGLSLPGVHTALDVERGMEFLQTEMGQRAREDEAGRDED"
     gene            257..655
                     /locus_tag="VHS97_21120"
     CDS             257..655
                     /locus_tag="VHS97_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX3450769.1"
                     /translation="MTKTDSLPIRSSTDIVLIRQEVRKWTGELGFSLVDQTKMITAAS
                     ELARNSLDYGGGGTVHFESLQDGIKRGLRLAFEDHGPGIPDIAQALSDGFTTGKGMGL
                     GLGGAKRLVNAFDITSRVGEGTRVTITRWK"
     gene            652..1692
                     /locus_tag="VHS97_21125"
     CDS             652..1692
                     /locus_tag="VHS97_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HEX3450770.1"
                     /translation="MINPHGESIGVIVDETTKVGEARRRTSWLAGQLGFDDTAQGKAA
                     LIVTEVANNLLKHAGGGELIIQGLDDDSHGEGLEIFALDSGRGINDVGQCLADGYSTA
                     GSPGTGLGAINRLVDSLQIYSNPGLGTVLWARVNRTPQPKSRSEPTLELGVVERAAPG
                     EQLCGDGWATNVRDGRTFVLMVDGLGHGSAAAQAAAEAVRVFRGHRAQEPNQIVESAH
                     LALRGTRGAALAIARLDPVRREVRYAGVGNISGVILDTTTGTSTSMVSQNGTVGYTIR
                     KIQTYEYPWNNDSLLLMHSDGVATQWSLDRYPGLRQRHPSLIAGALYRDYKRARDDIT
                     VLAARLWTRGKS"
     gene            1689..3524
                     /locus_tag="VHS97_21130"
     CDS             1689..3524
                     /locus_tag="VHS97_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450771.1"
                     /translation="MNMPLINLELRIEADIVLARQRARQIAALLGFAHLDQARIATAT
                     SEIARNAIQYAGGGRVEFVVETGAAPKMLIRILERGPGINDLNAILEGEYISPTGLGL
                     GIIGAKRLMDQFAVDTGAAAGATVSMSKTLPSRTSNITPQEIARLSAELARQAPSGLL
                     AELQQQNQELLNTLQELRDRQAEIAQIHSRELDETNRGVVALYAELEEGTKDLQRLSD
                     LKSRFLSEMSHEFRSPLNSIKGLTGFLLDRTDGDLSSEQEKQIQFIRQAADGLSTLVD
                     DLLDLARVEAGKAAIRVSSFEVRSLFESLHGMIRPMLDQGAVALVFEESTGIPNLNTD
                     EGKVAQILRNFLTNAVKFTERGEIRVSARRGQDDVVTFSVKDSGIGIAPENLNRVFEE
                     YGQIDNPLQNRVKGTGLGLPLTRKLAQLLGGSVSVQSDTGVGSTFSVAIPRVFPRDER
                     HEAGFTVLPAIDRLAALRAIEQRAAIEIALIVDDEERDRYLVKGALSALGAFDVVEAE
                     RGEEALLLARSHQPDVIFLDLILPDMSGFEILDVLKSEEKTRNIPVIIHTSEVLNDEK
                     RRRLNKDAVAIVTKGSKSREEAIAQIRDSLSKAGLRPPVHERAEF"
     gene            3526..>4542
                     /locus_tag="VHS97_21135"
     CDS             3526..>4542
                     /locus_tag="VHS97_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3450772.1"
                     /translation="MCKKSDTSILLVDDDDAKRYTIAKTLVRAGFEITEAGTGSEALS
                     LVALLPALVILDVKLPDISGFEVCRRIKSNPATCTIPVLHISTTFVDLEDKLHGLDGG
                     ADGYLTNVAEPLELLATVRALLRARRAEDAAQISTRQWQTTFDAISDGVMLLDASGKV
                     AQANRTIERILGRPWTDILGNELPALWDGPIQREESLFAKMLESGTRQADDRSLGDSW
                     IHVAVDPLRDVAGSIKGAICLVSDISDRKRMEMQLFAQAEDLQAAAQRKDEFLAMLAH
                     ELRNPLASLANSLQIIRMQVKGNVLVEDSVDVAGRQIHHMTRLLEDLFDVSRITRGTV
                     ELRKK"
BASE COUNT          985 a         1305 c         1362 g          890 t
ORIGIN      
        1 cgcggcgtac tgatcgacat ttcctcgctg gacatggttg actcgttcat cgggcgcatg
       61 atcggcaata tcgcaggcat gtcgcgcgtg cttgatgcca aaacggtcgt cgttggcatg
      121 cggccggccg tcgccatcac gctggtcgag ttaggtctgt cgcttcccgg cgtccacaca
      181 gcacttgatg ttgagagggg gatggagttc ctccaaaccg agatggggca gcgggccaga
      241 gaagacgagg ctggccgtga cgaagactga tagcctgccg atccgctcat caacggacat
      301 cgtcctgatt cgtcaagagg tgaggaagtg gaccggggag ctgggtttta gccttgtcga
      361 ccaaaccaaa atgatcacgg ccgccagtga acttgcgcgt aactcacttg attatggcgg
      421 tggcggaacc gttcacttcg aatcacttca ggatggcatc aagagaggac tccgacttgc
      481 tttcgaggat cacgggccgg gaattcccga tatcgctcag gctttgagtg acggctttac
      541 gaccggcaaa ggcatgggct tgggcttggg tggtgccaaa cggctcgtca acgcgttcga
      601 catcacatca cgagtcggcg aaggaactcg cgtcaccatc acgaggtgga aatgatcaat
      661 ccccatggag agtccattgg cgtcatcgtc gatgagacta ccaaggtggg agaggccagg
      721 cgacgtactt cgtggctggc agggcaatta ggatttgatg acacggcaca aggaaaagcc
      781 gcacttatcg tgaccgaggt cgcgaacaat ctgctcaagc atgccggtgg cggtgaattg
      841 atcattcagg gcctggatga cgattcccac ggggaaggcc tggagatctt cgccctcgac
      901 tcgggccggg ggataaatga tgtaggccag tgcttggccg atggttactc gaccgccgga
      961 tcgcctggga ccggcctggg tgccatcaac cgcctcgtgg actcgttgca aatctattcc
     1021 aatcctggcc tgggaacggt tctatgggct cgagtcaatc gaacgccaca gccaaaatcc
     1081 cggagtgaac ccacactcga gctcggcgtc gtcgaacgtg ccgcgccggg tgagcagctc
     1141 tgtggcgatg gctgggcgac gaacgtgcgc gacggccgca cctttgtcct gatggtcgac
     1201 gggctgggtc acgggtctgc cgcggcccag gctgccgcgg aggcggtgcg cgtcttccgt
     1261 ggccatcggg ctcaggagcc aaatcaaatc gtcgaatcgg cgcacctggc cctccgtggc
     1321 acgcgtggtg cggctctggc gatagccagg ttggaccctg ttcgccgcga ggtccgttac
     1381 gccggagtag gcaacatctc cggcgtgatc ctcgacacga cgactggcac gagcacgagt
     1441 atggtctcgc agaacggcac ggttgggtac acgattcgca agattcaaac ttacgaatat
     1501 ccctggaaca atgactcact tttactcatg cattccgacg gcgtggccac ccagtggagc
     1561 ctcgatcgct atcccggact gcgccagaga cacccaagcc ttatcgctgg cgccctttac
     1621 cgggattaca aacgagcgcg ggacgatatc acggttttgg ccgctcgtct atggacgagg
     1681 ggaaaatcat gaacatgccc ctcataaacc tagagctccg gatcgaggcc gacatcgtcc
     1741 tggccaggca gcgggcgcgg cagattgccg ccttattagg ctttgcccac ctcgaccaag
     1801 cgaggatcgc aaccgctaca tcagaaattg cccgcaacgc aatccagtat gcgggcgggg
     1861 ggcgagttga gtttgtcgtt gagaccggag cagcacctaa aatgctcatt cgcattcttg
     1921 agcgagggcc cggcatcaac gaccttaacg ccatcctcga gggtgagtac atctcgccga
     1981 cggggttggg actcgggatc atcggagcca aacggctcat ggatcaattt gcagtcgata
     2041 caggagccgc tgccggcgcc accgtctcga tgtcaaaaac tttgcccagc cgaacaagca
     2101 acatcacgcc acaggaaata gcgcggctct ccgcagaact ggcgagacag gcacccagcg
     2161 gactgctggc cgagctccag caacaaaacc aagaattact caatacattg caagaattgc
     2221 gggatcgcca ggcggagatc gcacaaatcc acagccgcga gctcgatgag actaaccggg
     2281 gcgttgtagc cctctacgca gagctcgaag aaggcaccaa ggacctacag cgtctttcgg
     2341 atctgaaatc ccgtttcctc tcggagatga gtcacgaatt ccggtccccg ctcaactcga
     2401 tcaaaggcct gactggcttc ctgctcgacc gaaccgacgg cgatctctcc tccgagcagg
     2461 agaagcagat tcaattcatt cgccaggccg ccgacgggct ttcgaccttg gtcgacgact
     2521 tgctcgacct ggcgcgagtc gaggctggta aggcggcgat ccgcgtgagc tcctttgagg
     2581 tacggtccct gtttgaaagc cttcatggaa tgattcgacc gatgctcgac cagggggcag
     2641 tcgctcttgt cttcgaggaa tcaacgggca ttccaaactt gaacacggac gagggaaagg
     2701 tcgctcagat cctgaggaac tttctcacca atgccgtgaa attcacggag cgcggggaga
     2761 tcagagtcag tgcccgccga ggtcaggacg acgtggtcac attctcggtc aaggatagcg
     2821 ggatcggaat cgcgcccgag aatttgaatc gagtcttcga ggaatacggg cagattgaca
     2881 acccgttgca gaaccgcgtc aaagggaccg gtctgggttt gccgctgacg cggaagcttg
     2941 ctcagcttct gggagggagt gtttcggtac aaagcgatac cggcgtgggc tcgaccttct
     3001 cggtcgccat tcccagggtg tttccccgtg acgaacgaca cgaagcgggt ttcaccgtac
     3061 tgcccgcaat cgaccgtttg gccgcacttc gtgccatcga acagcgcgca gcaatcgaaa
     3121 tagcattgat tgtggatgac gaggagcgag accggtatct tgtaaagggc gccctaagtg
     3181 ccctgggcgc gttcgacgtc gtcgaagcgg agcggggaga agaagccctt ctgcttgccc
     3241 gatcgcacca gcccgatgtc atatttctcg atctgatcct ccccgatatg agcggcttcg
     3301 agattctgga cgtgctcaag tcggaggaaa agacgagaaa catccccgtg atcattcaca
     3361 cgtcggaggt tctcaatgac gaaaagcgtc ggcgcctgaa taaggacgcc gtcgccatcg
     3421 tcacgaaagg atcgaaatcg cgcgaagagg ctatcgctca gattcgagac tcgctgtcaa
     3481 aggccgggct gcgtccgcca gttcacgaaa gggcggaatt ctgaaatgtg caagaagtcg
     3541 gatacctcga ttttgctcgt cgacgacgat gacgccaagc gatacacgat cgccaagacg
     3601 ctcgttcgcg cgggattcga gataacggag gccggaaccg ggagcgaggc gttaagcttg
     3661 gttgccttgc tacccgcact tgtcatcctc gatgtcaagc tgccagacat ttcggggttt
     3721 gaggtctgtc gtcgtatcaa gtccaacccg gcgacctgta ctatcccggt cctccacatc
     3781 tccacgactt tcgtcgatct cgaggataag ctgcacggtt tggacggcgg cgccgacggt
     3841 tatttgacca acgtcgcaga gcccctggag ctacttgcca ccgtgcgggc tctgctgcgc
     3901 gctcgcaggg ccgaggatgc cgctcaaatc tcgactcgcc agtggcaaac aaccttcgat
     3961 gccatcagcg acggcgtgat gcttctcgat gcatcgggca aagtggccca agccaatcgc
     4021 actatcgagc gtatcttggg gcgaccctgg accgatatct tgggtaatga acttcccgcc
     4081 ctgtgggatg ggccaatcca gcgcgaggag tcgctgttcg ccaaaatgct tgagtccggc
     4141 acgcgacagg ccgacgatcg ctccttgggc gacagctgga ttcacgtcgc ggtggatccg
     4201 cttcgagacg tggcaggctc gatcaaggga gcaatctgtc tggtgtcgga catttctgac
     4261 cgtaagcgta tggaaatgca gctcttcgcg caggccgagg accttcaggc ggccgcacag
     4321 cggaaggacg agtttctggc catgctcgcc cacgaactac gtaaccccct tgcatctctc
     4381 gcgaactctc tgcaaatcat ccgcatgcag gtcaagggaa atgtgctggt tgaggattca
     4441 gtcgatgtcg cgggacgcca gattcaccac atgacgcgcc tcctcgaaga tcttttcgac
     4501 gtctcacgaa tcacacgcgg caccgtagag cttcgcaaga aa
//
LOCUS       DATAQL010001226         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2416878, whole genome shotgun sequence.
ACCESSION   DATAQL010001226 DATAQL010000000
VERSION     DATAQL010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416878"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VHS97_21140"
     CDS             complement(<1..256)
                     /locus_tag="VHS97_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450773.1"
                     /translation="MGWFGNQSRTKNRRPHRLVIEQLDDRCLLSTAAPLNVTHHLTGH
                     ELVRRQALFSNSHHRPVQGERTGQLLKRIVVKSHDVERPAG"
     gene            922..1677
                     /gene="kdsB"
                     /locus_tag="VHS97_21145"
     CDS             922..1677
                     /gene="kdsB"
                     /locus_tag="VHS97_21145"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX3450774.1"
                     /translation="MEIVAVIPARFASTRLPGKALLSETGRPLIVHVIEAARRAKSLQ
                     RIIVATDDARIAQAVTSFGGEVMMTRADHATGTDRVAEVAARLDQARVIVNLQGDEPE
                     ISGHALDLVVELLENDSDAKVATLATPIRDESIYRDPACVKVVCDRRGRALYFSRSPI
                     PYHRDGLPDQRTTIAPLGHLHLGLYAFRRQFLLELGSLPSSALEAAEKLEQLRVLEAG
                     YSIAVGMVDEPSVGIDTPEDYKRFVARWRASQA"
     gene            1814..>2557
                     /locus_tag="VHS97_21150"
     CDS             1814..>2557
                     /locus_tag="VHS97_21150"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3450775.1"
                     /translation="MHKNVAWAGSIALAAAILGVGGAIAHGQDQGVLTRNDAVLVVDG
                     TVREIFRSPRRDRVDYLVQIEVKRSLAERMSPAPLRVAIPAPGDLVYVHASERSGNGQ
                     QERLAGNDPPAANARAVVPPERSQIRAYLTPGTRGGWVGAGRDWFEPTSSALAEANPS
                     DSAPAAPESGPVTPPSLPQSPPAGSKFALALLGLTGEIMNMKGQFVLRVSSVEQGGAA
                     QRSGLEPGDIIIGANNNALVGLDQLDQLAR"
BASE COUNT          476 a          811 c          759 g          511 t
ORIGIN      
        1 tgccagctgg gcgctcgaca tcatgcgatt tgacgacaat gcgcttcaac agttgacctg
       61 tcctctctcc ttgcacgggg cgatggtgag agttgctaaa gagagcttgc cggcgcacaa
      121 gctcgtgacc ggtcaagtgg tgggtcacat tgagtggagc ggccgtcgag agcaggcacc
      181 tgtcgtcgag ctgttcgatt acgagtcgat gaggccgacg gttcttcgtg cggctctggt
      241 ttccgaacca tcccatgaaa cgtgtctccc cagacggccg ccatgccatt ggctcaggcc
      301 gccccgcgaa tcggttgtga acgctctcct tccctggcgc actagacggc cgtccgagtc
      361 gactcggcac acggccactc tagcgcggcg accagcctcg aaaaaggatc gaaacttgtc
      421 gatcagagcg caagcaaatg ttgttagaga cggaccaccc taagcctcag ggccgaatgc
      481 tgatatcact atgattcagg cacgaatcgc cctggacctc gcggcggctt acccgagtgc
      541 agagaaccga catccttcgg aaacctcatg cggggaactg gggagccccc ccctatcatc
      601 gtctccggga acggcgattc ggatcagaca tcctgtggaa gactgggagg aatctttccg
      661 cccgatctat cgagctcgcg actacaatgg ccgagcccgt gccgacgctg tttgggtcgc
      721 gccggttttt gtgtgcacgc gtactcctct atacgctcag tgcgtgccca cgtttccagc
      781 cgggtttcct tctactccga cttgtgcctc atcttgagac cacctagagt agcagcattc
      841 cgcgactgca gctggtcgag cgcgagtctc gatcagtgcc tcttgtatcg gaccctctca
      901 cgggagatat tctttagaaa catggaaata gtcgccgtga tcccggctcg tttcgcttcg
      961 accagactcc ccggcaaagc ccttctatcc gaaaccgggc gacccttgat cgtgcacgta
     1021 atcgaggcgg cgcgacgggc aaaatcgcta caacgaatca tcgtcgcgac ggacgacgcc
     1081 aggatcgccc aggccgtgac cagcttcggc ggcgaagtca tgatgacgcg cgccgaccat
     1141 gcgaccggaa ccgaccgggt agccgaggtc gctgcccgtc tcgatcaagc tcgagtgatc
     1201 gtcaaccttc agggcgatga gcccgagatc tcaggccatg cgctcgatct tgtcgtcgag
     1261 ctcttggaaa atgactccga cgccaaggtc gccactctag cgacgccgat tcgcgacgaa
     1321 tcgatctatc gcgacccggc ctgcgtcaag gtcgtttgtg accgccgcgg ccgggcgctc
     1381 tacttttcac gcagcccgat tccctatcat cgtgacggcc tccccgatca acgcacgact
     1441 attgccccgc tcggtcactt gcatctgggc ctctacgcat ttcggcgtca attcctgctc
     1501 gagctcgggt cgctcccctc ctctgccctg gaggcggccg agaaactcga acagctgcgc
     1561 gtgctcgaag ccggttactc gatcgctgtc ggcatggtcg acgaaccaag cgtcggcatc
     1621 gacacccccg aggattacaa gcgatttgtc gcgcggtggc gggcgagcca ggcttaagcc
     1681 gtatcatccc gtcggagtcg gaagttgccg tacttgaacg gttcgttttc gatggcttat
     1741 catctgcgtg ggtcgttcta aaaacgtcgg gctgggtccc atcgcgggct tgcgcacaag
     1801 ccaggagctt acgatgcaca aaaacgtcgc ttgggccggg tcgatcgctt tggccgcggc
     1861 cattctgggt gttggagggg cgatcgctca cggccaggat caaggggtct tgacgcgtaa
     1921 cgacgcggtg ctggttgtcg acggcactgt ccgtgagatt ttccgcagcc cacgacgaga
     1981 ccgagtggac tacctcgtgc agatcgaagt aaagcgttct ctggcggagc gcatgtcgcc
     2041 cgcgccgtta cgcgttgcca ttccggctcc cggagacctg gtttacgttc acgcgtctga
     2101 gcgctcgggg aatggtcagc aagagcgcct ggccggcaat gatccgcccg ccgccaacgc
     2161 ccgagcggtc gtaccgcccg agcggtctca gattcgagcc tacctgacac ccggtacgag
     2221 aggcggctgg gtaggagcag gccgcgactg gtttgagccg acctcaagcg cgcttgccga
     2281 ggctaatcct tctgactctg ccccggcagc gcctgagtct ggcccagtca cgcctccctc
     2341 cctcccacag tcgccccccg ctggaagcaa gttcgcgctc gccctcctcg gattaacggg
     2401 cgagattatg aatatgaaag gtcagttcgt cttgcgtgtc tcgagcgtcg agcaaggggg
     2461 agcggcacag cggtcggggc tcgagcccgg agacatcatt atcggcgcca ataacaacgc
     2521 gctcgtggga ctcgatcaac ttgaccagct cgcgcgg
//
LOCUS       DATAQL010001227         6717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1805038, whole genome shotgun sequence.
ACCESSION   DATAQL010001227 DATAQL010000000
VERSION     DATAQL010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805038"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VHS97_21155"
     CDS             <1..1585
                     /locus_tag="VHS97_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3450776.1"
                     /translation="WSDWRDWQSELRATLTIDAHDIKRVVDAYLIEPGLTVGWSLPGA
                     GGEDAPATPGSVETFSDAPVAALASPAPLGLAAAARTVTAGRQDSATTPLPMAIPTGI
                     SRLVDYKPRRIILENGLRLIFERRPGTGIVALELFADAGLLREAKPGLACLTGRLLEE
                     GTKTRSAPELAEAIEDVGGTLESGATGSSVRVCSEDLPLAIELLADVTIRPVFPSDAV
                     SWVAERIAAEYRGDLEDPAFRAEMSFRSLVYGDHPLARDPRGGVREIARLTRQDTLEH
                     HQRHFAPENTVLVAVGDFDPRRLRSLVKAHLGTWEPRGLPRLPYPPVAKPGRGRVRRI
                     HHRGEQTHIVLGHLGVARTHPDFDSLVILDHIFGSGPGFCDRLGRIVRDELGLVYTIG
                     GGMTDSGDVLPGLFRVHAGTMPEQAERVISTVTEQIRAMHAGAFSDEEVDRARGYLTG
                     AWVFDYQSVEQRAERLLELERWGMSLDEPRHWPERIGAITPRQVRKAARTHLSPEALC
                     RVELGPPRRGSRRVEAECA"
     gene            1879..2952
                     /gene="plsX"
                     /locus_tag="VHS97_21160"
     CDS             1879..2952
                     /gene="plsX"
                     /locus_tag="VHS97_21160"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX3450777.1"
                     /translation="MRVALDAMGGDFAPGPIVTGACEAVGDHPELTVVLVGDRDRIEA
                     ELAKAPGVARDRLPIVHASQTIGMDEKPVDALRKKRDNSISRCWGLMAAADVKAVVSA
                     GNTGAMVASALFNAKMFLPGVRRPGIAAIFPSHQGPIVIIDVGANMNAKAEDLYQYGI
                     MGAIYAETILGVTCPRIGLLNVGTEDDKGNDLTKSTRALYQNSPWAARFVGNVEGRDI
                     YEGHVRVVICDGFVGNVLLKAGEGAVEFLFSTLREELARLMPHLPGDAGPKVAASLKR
                     LKTRFEYEEFGGAPLLGIRGACIICHGASGARAIKNALRVAGTMAEDRLGSLIVEQLA
                     GAPPADSGDVSGEVPASPNSSAG"
     gene            3073..3984
                     /gene="fabD"
                     /locus_tag="VHS97_21165"
     CDS             3073..3984
                     /gene="fabD"
                     /locus_tag="VHS97_21165"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX3450778.1"
                     /translation="MPKVAFLFPGQGAQFVGMGRELDQELPAVRALFDLAGEILGFDL
                     KQLCVEGPADALESTDVSQPAIYVASLAALESLKATKPELVEKCQGAAGLSLGEYTAL
                     AFADSMDFESGLKVVRQRGQAMQAAALATKSGMTSILGLDEPAVDELCRRAAPFGRIW
                     KANLLGPGNIVVSGDATALEHIDSIATELGAMKVVPLKVAGAFHSELMKPADDQLAKV
                     LGQTSVAAPRVPVYSNVDAIPHDNPEDIRRTLVAQVLQGVRWEDSIRRMLADGFDTFY
                     EIGPGRVLTGLLKRIDRKIPCTSIPAR"
     gene            4027..4692
                     /locus_tag="VHS97_21170"
     CDS             4027..4692
                     /locus_tag="VHS97_21170"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3450779.1"
                     /translation="MSIAQKKRTARIRLNRSSNGMLMTPEEFDAVTDHDDRFDYELVH
                     GVLVVVPPPGEAERDPNGELEYLLRLYRASHPRGSILDKTLSEQFIHLADSRRRADRV
                     IWVGLGRVPDIHKDVPAIVVEFVSNRKRDRVRDYEEKRREYLAIGVSEYWIIDRFKRT
                     LTVVRNAPGQPSEIVVQAEETYQSPLLPGFDLPLKQLLKLADDWAAANQKRKRDKNRE
                     QPN"
     gene            4931..5710
                     /gene="fabG"
                     /locus_tag="VHS97_21175"
     CDS             4931..5710
                     /gene="fabG"
                     /locus_tag="VHS97_21175"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX3450780.1"
                     /translation="MAEGSSATSAGCRVDLSGQIAIVTGASRGIGRAIAVKLAACGAT
                     VAGVARTLQGLEATLEAIRSAGGTAEGFAANVADSADVQRVVDEVEAKYQRIHVLVNN
                     AGVTRDGLMLRMDDSAWQEVIDTNLKGTFLFSRAVGACMMRGRYGRIINISSVTGLVG
                     NPGQANYSASKAGVIGFSKTIARELASRGITVNVVAPGFITTDMTNVLPEKIKTEVKE
                     RIPVRRLGTPEDIADLVTYLSSPSASYVTGQVIAVDGGMTA"
     gene            5959..6198
                     /gene="acpP"
                     /locus_tag="VHS97_21180"
     CDS             5959..6198
                     /gene="acpP"
                     /locus_tag="VHS97_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3450781.1"
                     /translation="MSVEERVIEIVSEQMGVSKDQVTRETSFVNDLGADSLDTVELVM
                     EFEEEFDITIPDEEAEKIQTVGQAIDYIDQHGHPK"
     gene            6289..>6717
                     /locus_tag="VHS97_21185"
     CDS             6289..>6717
                     /locus_tag="VHS97_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3450782.1"
                     /translation="MAHPRRVVITGMGLVTSLGDRLEPFWSALCAGRSGVGPLTLFDT
                     TEFKVKFGGQVRDWDPVARFGVKEARHLDRFAQFALAAAITAVDDAGIDFSTIPEEQC
                     GVMIGSGIGGLNEFESQHTVMMQKGPSRISPFVIPKLMVNA"
BASE COUNT         1325 a         2038 c         2087 g         1267 t
ORIGIN      
        1 ttggtccgat tggcgcgatt ggcagagcga attgcgagcc accctgacca tcgacgccca
       61 cgatatcaag cgcgtcgtgg atgcctacct gattgagccg ggcctgacag tcggatggtc
      121 gctcccgggc gcgggcggcg aagacgcacc ggcgacccct ggctcggtgg agacgttttc
      181 agatgcgcct gtggcggctc tggcatcgcc ggcgccgctg ggacttgccg cggccgcacg
      241 aacggtcaca gccggtcgcc aagacagcgc gacaaccccg ctcccgatgg caatacccac
      301 agggatctct cggcttgtcg actataaacc tcgacggatt atcctcgaga atggcctgcg
      361 actcatcttc gagcgacgcc ccggtacggg cattgttgcc cttgagctct tcgccgacgc
      421 gggcttgctg cgggaagcca aaccgggcct cgcctgcctg accggacgtt tgctcgagga
      481 agggaccaag acacgatccg cacccgagct ggccgaagcg atcgaagatg tggggggcac
      541 cctcgagagc ggcgcgaccg ggagctcggt ccgcgtttgc agtgaagatc tacccctggc
      601 aatcgaactg ctggccgacg tgacgatccg gccggttttc ccgtccgacg cagtgagctg
      661 ggtcgccgag cgaattgccg ctgaatatag aggcgacctg gaggatcctg ccttccgcgc
      721 cgagatgagc tttcgcagcc tcgtctacgg tgaccacccc ctggcgcgcg accctcgcgg
      781 cggagttcgc gaaatagcgc gcctcacccg acaagacaca ctggagcacc atcaacgtca
      841 ctttgctccg gaaaacaccg tcctggtggc cgtgggcgac tttgatcctc ggcggcttcg
      901 cagtctcgtg aaggcacacc ttggcacgtg ggaaccgcgc ggcctgccac gcctgccgta
      961 tcccccggtg gcgaaaccag gacggggtcg cgtccggcgc atccatcatc ggggcgaaca
     1021 gacacacatt gtattgggcc acctcggggt ggctcgcaca catcccgatt tcgacagtct
     1081 ggtgatactc gaccatatct tcggcagcgg tccggggttc tgcgatcgtc tggggcgaat
     1141 cgttcgcgac gaattgggcc ttgtctacac aattggcgga ggtatgaccg actcgggcga
     1201 cgttttgccc gggctgttcc gggtacacgc cggcacgatg cccgagcaag ccgagcgggt
     1261 catcagcacg gtcaccgaac aaatccgcgc gatgcacgcc ggggcgtttt ccgacgaaga
     1321 ggtcgatcgt gcacgcggct atctgaccgg tgcctgggtg tttgactatc agagcgtcga
     1381 acagcgcgcc gaacgcttgc tggaactcga acggtgggga atgagcctcg acgaaccccg
     1441 acactggcct gagcggatcg gggcaatcac gcctcgccag gtgcgcaagg ccgcgcgcac
     1501 tcatctgagc ccagaagccc tttgccgcgt cgagctcggc ccgccacggc gtggcagccg
     1561 ccgagtggag gccgaatgtg cttgaaagat tgcccgacga gcgcgccgct gattcgcgcg
     1621 cagcttgaca ggtctcttcc acgtcttcta cgatgacgcc tatgaatcgt gcgcggccct
     1681 ggcggcgggc tcgggcgcaa agcgggctcg acagccaggt tgggtgagaa atgccttttc
     1741 cgcgctgacc gccacgccgc gggaaaaaag cctcgcaatg cggaaaggga attgcctccc
     1801 ggtttccggc aatcgatttt tgttttcgat aatcataagt gcacggatgg ccaatcatca
     1861 tatattgcga gaggtgcgat gcgcgtagcc ttggacgcga tggggggcga tttcgctccc
     1921 ggtccgatcg tcactggagc atgtgaggca gttggtgacc atcccgagct gacggtcgtt
     1981 ctggtcggag accgcgaccg aatcgaggcc gaactggcca aagcgcccgg tgttgcccgc
     2041 gatcgtctgc cgatcgtcca cgccagccag accatcggca tggatgaaaa gccggtcgac
     2101 gccctgcgca agaagcggga taactcgatt tcgcgctgct ggggattgat ggccgcggcc
     2161 gatgtgaagg cggtcgtctc cgccggcaat accggggcca tggtcgcgtc ggccctgttc
     2221 aatgccaaga tgttcttgcc gggcgtccgt cggcccggca tcgcggcgat cttcccctcg
     2281 caccaggggc cgattgtgat catcgacgtc ggcgccaaca tgaacgccaa ggccgaagat
     2341 ctctaccagt acggcatcat gggggcgatc tatgccgaga cgatcctggg cgtaacttgc
     2401 ccgcgcatcg gcctcttgaa tgtcggcacc gaagatgaca aaggaaacga cctgaccaaa
     2461 tccacccgcg ccttgtacca gaacagcccg tgggcggctc gctttgtcgg caacgtcgag
     2521 ggacgcgaca tctacgaagg gcatgtccgg gtcgtgatct gcgacggatt cgtcggcaac
     2581 gtcctcttga aagccggcga gggggctgtc gagttccttt tctccactct cagggaagaa
     2641 ctcgcccggc tcatgcccca ccttcccggc gatgccggtc ccaaggtcgc cgccagcttg
     2701 aagcggctca aaacgcgatt cgagtacgaa gagttcggcg gtgccccgct tttggggatt
     2761 cgcggcgcgt gcatcatctg ccacggtgca tctggcgcgc gagcgatcaa gaacgcgctg
     2821 cgggtcgccg gcaccatggc cgaagatcgc ctgggcagcc tgatcgtcga gcaactggcg
     2881 ggtgctcccc ccgcggactc gggggatgtc tccggcgaag ttcccgcgtc gccgaattca
     2941 tccgcaggct gaaaccacag ggattttggg gcgttttttg cttgatgccg cccgcgtacc
     3001 tgtgccgccg tcttgggcgc ggggaatgcg acggcgttcc cgaccgactc attacttgag
     3061 gatctctttc ccatgcccaa ggtcgcattc ctgtttcccg gccagggtgc tcagttcgtc
     3121 ggaatggggc gcgagctcga ccaggaactc cccgcggtcc gggccctctt cgacctggcc
     3181 ggcgagatcc tgggtttcga tctaaagcag ctctgcgtgg aagggccggc cgacgcactc
     3241 gaatcgaccg acgtcagcca gccggcgatc tatgtcgcga gcctggcggc ccttgaaagc
     3301 ctgaaggcca ccaaacccga gctcgtcgaa aaatgccagg gagccgccgg attgagcctg
     3361 ggcgaataca cggccctggc ctttgccgac tccatggatt tcgagtcggg tctcaaggtt
     3421 gtccgtcagc ggggtcaggc catgcaagcc gccgccctgg ccacgaaaag cggcatgacc
     3481 agcatcctgg gcctggacga gcctgccgtt gatgagctct gccgccgcgc cgctcccttc
     3541 ggccgaatct ggaaggccaa cttgctgggg ccgggcaata ttgtcgtctc aggggacgca
     3601 acggctctgg aacacatcga ttcgatcgcc accgagctcg gcgctatgaa ggttgtaccg
     3661 ctcaaagtcg caggagcgtt ccactccgaa ctgatgaagc ccgctgacga ccagctcgct
     3721 aaggtactcg gccagacgag cgtcgccgcg ccgcgcgtcc ctgtctactc caacgtcgac
     3781 gccatacccc acgataaccc tgaagatatc cgccgcaccc tggtcgccca ggtgctccag
     3841 ggagttcgct gggaagactc gattcgccgg atgctcgccg atggcttcga cacgttctat
     3901 gagatcgggc ccggccgtgt cttgaccgga ctgctcaaac gcatcgaccg caaaatcccg
     3961 tgcacgagta ttccagcgcg gtaaaatggg tcgaggtttc ccctcgaatg gcacacggtg
     4021 cgaaccatga gcatcgccca gaagaaaaga accgcgcgga tcaggctcaa ccggtcttcc
     4081 aacgggatgt tgatgacacc ggaggaattc gacgcggtca ccgatcacga cgaccgattc
     4141 gactacgagc tcgtccatgg agtccttgtc gtggtcccac ctcccggtga agcggaacgc
     4201 gatcctaatg gcgagcttga atatcttctt cgtctctatc gagcgtcgca cccgcgagga
     4261 tcgatcctgg acaagacgct cagcgagcaa ttcatccact tggctgacag ccggcggcgg
     4321 gccgatcgcg tgatctgggt ggggctggga cgcgttcccg atatacacaa ggacgtcccg
     4381 gcgatcgtcg tcgagttcgt gtcgaatcgc aagcgcgacc gtgtacgtga ctacgaggaa
     4441 aaaagacgag agtatctggc catcggtgtt tccgagtatt ggatcatcga ccgattcaaa
     4501 cgaacattga cggtcgtcag aaacgccccg ggacaaccct cggaaatcgt ggtgcaggcc
     4561 gaggaaactt atcaaagccc gttgttaccc ggcttcgatc tgcctttgaa gcaattgctc
     4621 aagctagcgg acgattgggc cgcagcaaac cagaaacgga agcgcgacaa gaaccgggag
     4681 caacccaatt gatgtctacc aacccggcgc actcgagaca tcaggcacac tgggggacat
     4741 agcaaatttg tcaaacctcg tatggtctta ttttggggtt gctggctgcg aacagccctg
     4801 attataacgg cattcgattt gcttctacgc tgaagaatga taagaccttg aggcgtttct
     4861 gccgcgaccg tgccgatatc gtcccccttt tttatgttga ccataatgct cttccaggga
     4921 tgttacgatc atggctgagg gatcgagcgc cacgagcgcg ggctgtcgcg tcgacttatc
     4981 gggacagatt gcaatcgtga ctggcgcatc gcggggaatt ggccgggcga tcgcggtcaa
     5041 gcttgcggcc tgcggtgcga ccgtggcggg cgttgcgcgt accctgcagg gactggaagc
     5101 gacgctcgag gcgattcgca gtgctggcgg gaccgccgaa ggctttgccg ccaacgtggc
     5161 cgactcggcc gacgtccagc gcgtggtcga cgaagtcgag gcgaaatatc agcggattca
     5221 tgtcctggtc aacaatgcag gagtcacacg cgatggtctt atgctgcgga tggacgacag
     5281 cgcctggcaa gaggtcatcg acaccaacct gaagggaacg tttctcttct caagggcagt
     5341 cggcgcctgt atgatgcgcg ggcgatacgg gcggatcatc aatattagta gtgttactgg
     5401 attggtgggc aatcccggtc aggccaacta ctcggccagc aaggcgggcg tgatcggctt
     5461 cagcaagacg atcgcccgcg aactggcttc ccgcggaatc acggtgaatg ttgttgcacc
     5521 gggattcatc accaccgata tgacgaacgt cttacccgaa aagatcaaaa ccgaggtcaa
     5581 ggagcgcatc ccggttcgcc ggttgggaac gcccgaagac atcgccgacc tggtcactta
     5641 cctttccagc ccgagcgcga gctatgtaac tggccaggtg atcgcggtgg acggcggaat
     5701 gacggcgtga cgaattgccg cggtgtctca ctcgacatcg tcgacctatt gcgggatgcg
     5761 tttcatggcg gccggacgac ttgtcgccgc ctgaaccgaa aatcgacttc cgcgcttggc
     5821 cgcaagacgt gacgtagagc ccgttaccgc atgccacgtt caatggacaa tcagggcagg
     5881 gaatgctaag ttgagtccta tactcgcaga tcgttgaaaa gtgcctgatg gtatggcagt
     5941 agacgaggag gttcccccgt gtccgtggaa gagcgcgtga tcgaaatcgt cagcgaacag
     6001 atgggagtct ccaaagacca ggtcaccagg gagacctcct tcgtcaacga cctcggggcc
     6061 gactcgctcg acaccgtgga actggtgatg gagtttgaag aagagtttga tatcacgatc
     6121 cccgacgaag aggccgaaaa gattcagacc gtcgggcagg ccatcgacta cattgaccag
     6181 cacggccatc ccaagtagac gggcttggtc cctgatcaaa ccgggcatga aaagaggctc
     6241 agttactgtt caccattcca cgacggtgtt ctaacaatca gtaagatcat ggcacatcct
     6301 cggcgcgttg taatcaccgg catgggtttg gttacatcgc tgggtgatcg cctcgagccg
     6361 ttctggtccg ccctttgcgc cgggcggagt ggcgtcgggc cgctcacact tttcgatacc
     6421 actgagttca aggtcaagtt cggcggccag gtccgcgact gggacccggt tgcccggttt
     6481 ggcgttaaag aagctcgaca cctcgatcgt ttcgcccagt ttgccctggc cgcggccatc
     6541 acggccgtcg atgacgccgg gatcgatttc tcgaccatac ccgaagagca atgcggagtg
     6601 atgatcgggt cgggcatcgg gggacttaac gaattcgagt cgcagcacac cgtgatgatg
     6661 cagaagggtc cgtcgcggat cagcccgttc gtaatcccca agctgatggt caacgcc
//
LOCUS       DATAQL010001228         5678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4093393, whole genome shotgun sequence.
ACCESSION   DATAQL010001228 DATAQL010000000
VERSION     DATAQL010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4093393"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VHS97_21190"
     CDS             complement(<1..1015)
                     /locus_tag="VHS97_21190"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX3450783.1"
                     /translation="MKVLVANLGSTSFKYRLFDMSNPDEPVLARGAIERIGSPTAKVL
                     IKSARGERELVCPIADHGEAVQLCLDQMTDPEVGVVRDASEVAAIGFKAVHARNLTGV
                     HLVDETVLAAMEAFADVAPAHNPPYTKAMRMLRDRFPRLPLVAAFETGFHRTIPEAAQ
                     RYAIPDTWATELGVRRWGFHGASHRYIAGRMPELLGRSDIKLISCHLGGSSSLSAIKN
                     GQSIACSLGMSPQSGLPHNNRVGDFDVFALPVIMRETGKTLDEVLSILANQSGLEGLS
                     GAGCDLRDIEAAAAAGDPKAELAIEVFVTAIRHYLGAFLLELDGADAIVFTGGIGENS
                     ERIR"
     gene            complement(1012..1299)
                     /locus_tag="VHS97_21195"
     CDS             complement(1012..1299)
                     /locus_tag="VHS97_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX3450784.1"
                     /translation="MNGNALGLIETLGLVGLIYAVDAMLKAASVELASPVIKLDGGIV
                     SVMVQGDVSSVRAAVEAGAEAAAKVGELKAAHVIPRPDSAVVRAFAGGSAR"
     gene            complement(1472..1798)
                     /locus_tag="VHS97_21200"
     CDS             complement(1472..1798)
                     /locus_tag="VHS97_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX3450785.1"
                     /translation="MATAERSRQRISGNGSTSNGEALGLIETKGLVGMIEATDAMLKA
                     ANVHLAGRVQIGGGFVTTLVKGDVGSVRAAVESGAEAAGKVGELVSAHVIPRPDEVIL
                     RTFLGS"
     gene            complement(1803..2138)
                     /locus_tag="VHS97_21205"
     CDS             complement(1803..2138)
                     /locus_tag="VHS97_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX3450786.1"
                     /translation="MAGSTIEALGMIETKGFVALVEASDAMVKAANVELVGWDKIGSG
                     LVTAFVAGDVAAVKAAIDAGAAAASRIGEVVSVQVIPRPHEDLGGVLTFAKASQTAAS
                     SKNPKSDGA"
     gene            complement(2226..2915)
                     /locus_tag="VHS97_21210"
     CDS             complement(2226..2915)
                     /locus_tag="VHS97_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HEX3450787.1"
                     /translation="MSTLTAPSRDTVEAMVRSILARQTAAAGATNGRAAAGITPKVVV
                     NISARHCHLTQADVDVLFGKGYQLSPLKRLYQDTDFAANETVAVVGPRQRMIPGVRIL
                     GPCRKFSQVELAFTDAISLGIDVPVRLSGDIEGTPGCLLIGPVGTLVLSKGVIRAERH
                     VHMGPADAQFYGVKHLDRMNMRVDGPCPTTLEGLLVRTHADWKLEVHIDTDEANCCDL
                     PHASRVTLTKA"
     gene            complement(2912..3712)
                     /locus_tag="VHS97_21215"
     CDS             complement(2912..3712)
                     /locus_tag="VHS97_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX3450788.1"
                     /translation="MLDLITRQGYATLDELVKVLGVSESTIRRDLEALDLAGSIKRTH
                     GGAVYTGEVRSMPAFDERTETAAAEKRAIGLAAAALLKDGDTVLLDGGTTTLEVARAL
                     IGRRVQVVTNSLPIAQLLASSQQTDLILIGGYIYPRTGVALGPLAIATMQSIRVRTAI
                     LGAGGIVAEGIYNSNSLLVETERQMMLCGQEVMIVADHTKFGRLALARLCGLDEVDQV
                     VVDSGVIARDRQMLEGAGVAIHVAPVADHRTNGAIQEPVRGDSMRNDS"
     gene            3936..4769
                     /gene="mutM"
                     /locus_tag="VHS97_21220"
     CDS             3936..4769
                     /gene="mutM"
                     /locus_tag="VHS97_21220"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX3450789.1"
                     /translation="MPELPEVETMVRGLRPAIEGRKLRRIEVLDSFLVQGCPAAHLAR
                     YGRGATVSEVVRRGKWVVVTLAGQKGIIVIQPRMTGSFWLASPDRPEHIRLVFHVDKP
                     RAMIWYCDARRLGKIGWYSGAEEAGRAFARSHGPDALEIGRDELASRLSRTGRAIKPT
                     LMDQKVLAGIGNIHADEILHASRIHPERSAATISVAELDRLHPAIRQVLTTAIRLEGS
                     SFDAGYRTVLGREGGFLAQNSVYGRAKLPCRACSRPIVKTKIAGLIGRPTYLCPFCQI
                     R"
     gene            complement(5043..5225)
                     /locus_tag="VHS97_21225"
     CDS             complement(5043..5225)
                     /locus_tag="VHS97_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450790.1"
                     /translation="MKKATENRLTKLADAAFEQAARKVIKRANECGTVVIVWEDEELK
                     KVDPRKISKPKPKTAS"
     gene            complement(5307..5525)
                     /locus_tag="VHS97_21230"
     CDS             complement(5307..5525)
                     /locus_tag="VHS97_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450791.1"
                     /translation="MNRAVGLDQEKHELSKQQPKPPTVYVIAGPNGEGKTTFASEFLP
                     DFVKCREFLNADLIASGGCTIHPDFRRS"
BASE COUNT         1045 a         1772 c         1740 g         1121 t
ORIGIN      
        1 agcggatccg ttccgagttt tcgccgatcc cgcccgtgaa gacgatcgcg tcggcgccgt
       61 caagctcgag caaaaaggcg cccaggtaat ggcgaattgc ggtgacgaag acctcgatgg
      121 ccagctccgc cttggggtcg cccgccgcgg ccgcggcctc gatgtcccgg agatcacacc
      181 ctgctccgct cagcccctcg agcccggact ggttcgccag gatcgatagc acttcgtcga
      241 gcgttttgcc ggtctcgcgc atgatcacgg ggagggcgaa aacatcgaaa tctccgactc
      301 ggttattgtg cggcaaaccc gactgcgggc tcatccccag gctgcacgcg atcgattggc
      361 cgtttttgat tgccgagagt gatgagctac cacccagatg gcaagagatc agcttgatgt
      421 cggaccggcc caaaagctcg ggcatccggc cggcgatgta gcggtggctc gcaccatgaa
      481 acccccaccg gcggacgccg agctcggtcg cccaggtgtc gggaatggcg tagcgctggg
      541 cggcctcggg aatcgttcgg tgaaatccgg tttcgaaggc ggcgaccaag gggagccggg
      601 gaaaccgatc ccggagcatt cgcatcgcct tggtgtacgg cggattgtgc gccggcgcga
      661 cgtcggcaaa ggcttccatc gcggccagca cggtctcgtc aaccaggtga actccggtca
      721 gattgcgagc gtgaacggcc ttgaaaccga tcgccgcgac ttcagacgcg tcgcgcacca
      781 cgccgacctc ggggtcggtc atctggtcga ggcagagctg gacggcctca ccgtgatcgg
      841 cgatcgggca gacaagctct cgttcgcctc gtgccgactt gatcaagacc ttggccgtgg
      901 gcgacccgat acgctcgatg gcaccccgcg ccaggaccgg ctcgtcgggg ttgctcatgt
      961 cgaataagcg gtacttgaag ctcgtgctcc ccagattggc cacgaggacc ttcaccgtgc
     1021 cgaccctcca gcgaaggcgc ggacgacggc ggagtccggc cggggaatga cgtgggcggc
     1081 cttgagctcg ccgaccttgg cggcggcctc agctccggcc tcgaccgcgg cccgcacgct
     1141 gctgacgtcc ccttggacca tcacgctcac gatgccgccg tctagcttga ttaccggact
     1201 ggccagctcg acgctggccg ccttgagcat cgcatcaacc gcatagatca acccgaccag
     1261 accgagcgtt tcgatcaggc ccagggcgtt tccattcatc tgcttggctc ccgcaattcg
     1321 atagtggcat gagcactgat ttttgtgtca cggccttccg ggccgtgtcc ggccgcgggc
     1381 aggatgcccg ttccgctgca agaaaacaca cgggcaggat gcccgtgcca ctgcaagaaa
     1441 acacacgggc aggatgcccg tgccatacgg ttcacgatcc caggaacgtc cgcaagatga
     1501 cttcatcggg acgaggaatg acatgggcgc tgacgagttc gcctaccttg ccggccgcct
     1561 ccgcgcccga ctcgacggca gcgcgaacgc tccccacgtc tcccttgacc agcgtggtga
     1621 cgaaaccgcc gccgatttga acgcggcccg ccagatggac gttggccgcc ttgagcatgg
     1681 cgtcggtcgc ctcgatcatg ccgaccaagc ccttggtctc gatcaggccc agcgcctcgc
     1741 cattactggt gctgccgttg ccgctgatac gctgtctgct ccgctctgct gtggccatgg
     1801 ggtcaggctc cgtcactctt gggattcttg gaggaagcgg ccgtttgtga cgctttggcg
     1861 aatgtcagca caccgcccag atcttcgtga ggcctcggaa tcacctggac gctcacaacc
     1921 tcgccaatgc ggctggcagc ggcggctccg gcatcgatgg cggccttgac agcggccacg
     1981 tcgccggcga caaacgctgt taccagtccg ctgccgatct tgtcccaacc aaccagctcg
     2041 acgtttgctg ctttgaccat tgcgtcgctg gcttccacca gggcgacaaa acccttggtt
     2101 tcaatcattc ccagcgcttc gatcgttgat ccggccatac aggccctcct tctctttctt
     2161 gggtcagctc gggtcttctt gaacgtgtcg tctttgcacg gagatcagtg tgaaattgtg
     2221 tgtcatcaag ccttggtcaa tgtgactcgg ctggcatgcg gtaagtcaca gcaattcgcc
     2281 tcatcggtgt cgatgtgcac ctcgagcttc cagtcggcgt gggtgcgcac caagagccct
     2341 tcgagcgtgg tcgggcacgg tccatcgacc cgcatgttca tccggtcgag atgcttgacg
     2401 ccataaaact gggcatcggc cggtcccatg tgcacatgcc gctcggcgcg gatgacgccc
     2461 ttcgagagaa ccagggtgcc cactggtccg atcaagaggc agccgggagt tccctcgatg
     2521 tcgcccgaga gccgcacggg gacgtcgatc cccaggctga ttgcatcggt aaaggccagt
     2581 tcgacctggc tgaatttccg gcaaggcccc aagatgcgca ctcccggtat cattcgttgt
     2641 cgaggcccga caacggcgac ggtttcgttg gcggcgaagt cggtatcctg gtagagccgc
     2701 ttgagcggcg aaagctgata gcccttgcca aaaagtacgt cgacgtcggc ttgagtcagg
     2761 tggcagtgcc gcgccgagat gttcacaacg accttgggcg ttatgcccgc ggccgcgcgg
     2821 ccattggtcg cgcccgcagc ggcggtttgc ctcgctaata tcgaacgaac catcgcctcg
     2881 acagtgtcgc gcgagggtgc ggtcaaggtg ctcatgagtc gttcctcata ctgtcgccgc
     2941 gaaccggctc ctgaatcgcc ccgttggtac ggtggtcggc gacgggcgcc acgtggatcg
     3001 caaccccggc cccttccagc atctgtcggt ccctggcaat cacaccggaa tcgaccacga
     3061 cctgatcgac ctcatccagc ccgcataggc gtgccagggc cagccggcca aatttcgtat
     3121 ggtcggcgac gatcatgact tcctgcccac acaacatcat ctgccgctcg gtctcgacta
     3181 agagcgagtt ggagttgtaa attccttcgg ccacaatccc cccggcgcca aggatggcag
     3241 tgcgaacgcg aatcgattgc atcgtcgcga tggccagcgg cccgagtgcg acgcctgtcc
     3301 gagggtagat gtaacccccg atcaagatca ggtcggtctg ctggctcgac gccaagagct
     3361 gggcgatcgg caaactgttg gtaacaacct gaacccgcct gccgatcaaa gcccgggcga
     3421 cttcgagcgt cgtcgtgccg ccgtccaaca gaacggtgtc gccatccttg agcagagctg
     3481 cggccgccag accaatggcg cgcttttcgg cggctgccgt ctcggtgcgc tcgtcaaaag
     3541 cgggcatcga cctcacctcg ccggtgtaga ccgccccccc atgggtccgc ttgatcgatc
     3601 cggccagatc gagtgcttcc aggtcgcgcc tgattgtgct ctcggagacg cccagaacct
     3661 tcaccagctc gtcaagggtc gcataaccct gccgagtgat cagatcgagc aaatttcggc
     3721 gtcgagtctc ggcaagcatg cccacccttg gacgaaaaca ctcttggtat ccgtcagtag
     3781 tcttcactat cgaacagaat ttgtcaagag acttcacaga agatggtgga ttatgaagga
     3841 acatggtaga tttcgatcgg ttggtggtct cgatgcggcg gtgccggtag tatcgatggg
     3901 gtgagggatg cctcggcttt gaggggatga tgacgatgcc agagctgccg gaagtcgaaa
     3961 cgatggtccg ggggctgcga cccgcgattg aggggcggaa actacggcgg atcgaggttc
     4021 tcgactcgtt cttggtgcaa gggtgtcccg cggcgcacct ggcgcgttac ggccggggcg
     4081 cgacggtgag cgaggtggtt cggcgtggga agtgggtcgt cgtgaccttg gccggacaga
     4141 agggaatcat cgtcattcaa ccacggatga cgggaagctt ttggcttgcg agtcccgatc
     4201 gacccgaaca catccgcctg gtgttccacg tggacaaacc gcgtgcgatg atctggtatt
     4261 gcgacgcgcg gcggctaggc aaaatcggct ggtacagcgg ggccgaggag gcagggcgtg
     4321 cgtttgctcg ttcccatggg ccggatgcgc tcgaaatcgg ccgcgacgag ctggcatcac
     4381 ggctcagccg caccggacgc gccatcaaac ccacgttgat ggaccaaaaa gtcctggccg
     4441 gaattggcaa catccacgct gacgagatcc tgcacgcttc ccggatccac cctgaacgga
     4501 gcgcagccac catttccgtg gccgagctag ataggttgca cccggcgatc cgtcaggtat
     4561 tgaccacggc gatcaggctc gaggggtcga gcttcgacgc cggttaccga acagtgctgg
     4621 gccgggaggg cgggttcctg gcgcagaatt ccgtgtacgg ccgcgccaaa ctaccctgcc
     4681 gggcgtgctc tcgaccgatc gttaagacca agattgccgg attgatcggg cggccgacgt
     4741 atttgtgtcc gttttgccag atccgctaga gtgtcgtccg tcttttaagc cggatgcata
     4801 gcggtcgggg gaggttggat cgattaaggt cggctgtcgg acgggcggtt ctcccctctt
     4861 tctggcggct tcctcccaac gtagcagagc gtgtacaaac aaagcatgga ggcggccatg
     4921 accctcacac ccgaattgaa gcaagcggtc gagaaggccg gcgacgagcc tgtccgagtt
     4981 gaagagccgg aaacgcatac ggcgtatttg cagacggatt ctcaccaaac gcagtagccg
     5041 attcatgacg ctgttttcgg tttcggtttg cttatctttc gtggatcgac cttcttcaat
     5101 tcctcgtctt cccagacgat tacgaccgta ccgcactcgt tggcacgttt gatgaccttc
     5161 cgggcggcct gttcgaacgc ggcatccgcc agtttcgtca gacgattctc agttgctttc
     5221 ttcatgatat tccgccgatt gctgttcgat gcgccgatag agccgctttt gcttgacgac
     5281 caacggcccg tctccctcga cggcaatcag cttcggcgga agtccggatg aatcgtacaa
     5341 ccaccagagg ctatcaagtc cgcattgagg aactcccggc attttacgaa gtccggcagg
     5401 aattccgaag caaatgtcgt cttgccctcc ccattgggac cagcaatcac gtagaccgtg
     5461 ggaggcttgg gctgctgctt gctcaactca tgcttctctt gatcgagtcc cacggcacgg
     5521 ttcaacttta gcatcctcgt aggtcacaac agctgggtgg ctggggtcga gctggcgaca
     5581 gccagcgagc ccccggtgcg gaagccttgg atcttgcggc ttcgccgttc gggcgtcgac
     5641 cccagccacc ccctgagttg taacctactc ttgaacct
//
LOCUS       DATAQL010001229         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5467759, whole genome shotgun sequence.
ACCESSION   DATAQL010001229 DATAQL010000000
VERSION     DATAQL010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5467759"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VHS97_21235"
     CDS             <1..921
                     /locus_tag="VHS97_21235"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450792.1"
                     /translation="VGKAHYQQKRYDLAETCWKEALELDPTVPEAGWALIDLMDFEAR
                     AEEAHRLGMRLFEVEPDPRDRVRLLLEMSRLDIDKVAPGSTVQVFEPVWRQYPAYLPL
                     ALAVGLALVHNSQSVEGIDVLRDALERHPDSAEAWDGWLTGLDEGYQPDLLRREFARL
                     PKSLAGDPRFAKHEGKVSQGAHDWPRAVNAYRRAYAFEPFNGVVLYRLRMALRAAGET
                     FEFHRADELFTVYQSAFRQMRPVYDEAFAIKTLGLEPRTELYHRLAGLREQLGRFDEA
                     RAWHRLVLRDFPDDAHSLAALARLKQQNLE"
     gene            complement(968..1834)
                     /locus_tag="VHS97_21240"
     CDS             complement(968..1834)
                     /locus_tag="VHS97_21240"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3450793.1"
                     /translation="MSTKKARSYEKNFEVAAPVESVWKAITEGDELTRWFCQEARCEP
                     DIGGQQHIDWGGGAKATGVITVWKPNSHLRTEAVRPDLDNSMSAGTSEPYATDWYLEH
                     QGGVTRVRMVASGFGEGPDWDHEYDGTFHGWDMFHKTMKHYLEHHRGHASANVVLYAV
                     LSVPPSEAWSRLMSAEGLIKSGSLGDLIIGAPFRFETSQSDPFAGVVHNYVPGKTFSA
                     MVENLNKAILNIELSSVPGQGHVLYVSLNTWGLPKPDVDALAAHLKAIVYGLFPQKTE
                     TPYSACAVNENG"
     gene            2022..2094
                     /locus_tag="VHS97_21245"
     tRNA            2022..2094
                     /locus_tag="VHS97_21245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2056..2058,aa:Glu,seq:ctc)
     gene            2203..2463
                     /locus_tag="VHS97_21250"
     CDS             2203..2463
                     /locus_tag="VHS97_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX3450794.1"
                     /translation="MIAIELYGVARLRAGTGFVRLEASTIGQALRELGRVCPALEGSV
                     LNRGTVEPVYKLSLNSDRFVSDPDTPLDDGDVLLLVSADAGG"
     gene            2468..>3693
                     /locus_tag="VHS97_21255"
     CDS             2468..>3693
                     /locus_tag="VHS97_21255"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3450795.1"
                     /translation="MVPGYHGRYLRVDPRPGRTGCVALEERVLRHFLGGVGLGAWILA
                     HETSPGVDPLGPEAALVFALSPLVGSPLTTSAKFAVVAFSPLTGRVCDALSSSHFAIA
                     AKRTGVDSLVVTGSCDVPSIVLIDGTDRVEPTVQWLPASSLWGLSAQEAEERIRSQHG
                     REWQVAAIGPAGERLIPFATLSHDGRHAGRGGLGAVLGSKRIKAIAVRGNHLSQPADP
                     AQTIALAKKYARKSFGPATEKYRELGTVANLLVFNRFDALPTRNFQASSFEGADLLSA
                     AELAPARRIARASCASCTIGCEHIYAAGSGDSGGVRLEYESLYALGPLCGVDQPAAVL
                     AAAKACDDAGIDTISTGATIAFLMQCAERGLVGERLEPSQRPLRFGDGVAVREAIDAL
                     VARRGTLGELLALGSRR"
BASE COUNT          609 a         1129 c         1218 g          737 t
ORIGIN      
        1 gtgggcaaag cccactacca gcagaagcga tatgacctgg ccgagacctg ctggaaagag
       61 gcgctggagc ttgatccgac cgttcccgag gcgggctggg cgttgatcga tctcatggac
      121 ttcgaggcaa gagccgaaga agcccaccgg ctcggcatgc ggctctttga ggtcgagccg
      181 gatccccgcg accgcgtccg attactgctg gagatgtccc gcctcgacat cgacaaggtc
      241 gctcccggat cgaccgtgca ggtcttcgag ccggtctggc ggcagtatcc cgcgtacctt
      301 cccctggcac tcgccgtggg tttggccctg gtgcacaaca gccagtccgt cgaggggatc
      361 gacgtgctcc gcgacgccct ggagcgccat cccgattcgg ccgaggcctg ggatggctgg
      421 ctcacagggc tggatgaggg ctatcagccg gatctgctca ggcgagagtt cgcccgtctt
      481 cccaagagcc tggccggcga cccccggttc gccaagcatg aggggaaagt gtcgcaaggg
      541 gcccatgatt ggcccagagc cgtcaacgcc taccgccgcg cctatgcctt cgagccgttc
      601 aatggagtcg tgctctaccg gctccggatg gcgctgcgag ccgcaggcga gacgttcgag
      661 ttccaccgtg ccgacgagtt gttcacggtc taccagagtg ccttcaggca gatgcggccg
      721 gtctatgacg aggcgttcgc gatcaagacg ctcgggctgg agccgcgcac ggagctgtac
      781 catcggctgg ccggactcag agagcagttg gggcgtttcg acgaggcccg agcctggcat
      841 cggctggtgc ttcgcgactt ccccgatgat gcacacagcc ttgccgccct ggcgcggctc
      901 aagcaacaaa acctcgaatg atgtgatttg cgaccgcacg catcgagaca gctcggcctc
      961 ggagatgtca cccgttctcg ttcaccgcgc aggccgaata cggcgtctca gtcttctggg
     1021 ggaagagccc gtagacgatc gccttgagat gggcggccag cgcatccaca tctggcttgg
     1081 gcaagcccca cgtgttgaga gacacgtaga ggacgtggcc ttggccggga accgagctca
     1141 actcgatgtt gaggatcgct ttgttgaggt tttcgaccat ggccgagaac gtcttgccgg
     1201 gaacgtaatt gtgcacaacg ccggcgaagg ggtcgctttg gctggtctca aagcggaacg
     1261 gcgcgccgat gatgaggtcg cccaagctgc cgctcttgat caggccctcg gcgctcatca
     1321 agcgcgacca agcttcgctc ggggggacgg aaaggacggc atagagcacc acgttagcgg
     1381 acgcgtggcc gcggtgatgc tcgaggtagt gcttcattgt cttgtggaac atgtcccaac
     1441 cgtggaacgt tccgtcgtac tcatggtccc aatccggccc ctcgccgaag ccggaggcca
     1501 ccatgcggac gcgcgtaacg ccaccttggt gttcgagata ccagtcggtc gcgtatggct
     1561 ccgacgtccc cgcactcatc gaattgtcca gatcgggccg gacggcctcg gtgcgaaggt
     1621 gagagttggg cttccagacg gtgatcacgc ctgttgcctt ggcacccccg ccccagtcga
     1681 tgtgctgctg gccgccgatg tccggctcgc acctcgcctc ctggcaaaac cagcgcgtca
     1741 gttcatcacc ctcggtgatg gccttccaga cggactcgac cggggctgcg acctcgaagt
     1801 tcttttcgta cgaacgggcc ttttttgtac tcatggcggt aactccttgg ttggattcgg
     1861 aggcaagatt tcgtcctctt cgtcggtctt ggtgatcgcc gggtaagccc ccagcaggaa
     1921 tcggaacaaa cgccctttgg gggcatgatc gtcatggtac tcttgcgaac ccggttactt
     1981 gactgatttc gcgcgcggct tatgataaga tttatcttct ggcccccatc gtctagcggc
     2041 ctaggatact tggttctcag ccaagggacc ggggttcgag tccccgtggg ggtgcttcga
     2101 gggcgtttca ttcttacagg cgaattccgc ccgattttcc cgtctcgatg cttttcgacg
     2161 gaatcggacg agggttctcg gtggaggctc aactccccct ccatgattgc catcgagttg
     2221 tacggcgtcg ctcggctccg cgcgggcacc ggtttcgtcc ggctggaagc atcgacaatc
     2281 ggtcaagcac tgcgcgagct tggccgcgtt tgcccggcgc tggaaggctc ggtcttgaat
     2341 cggggaacgg tcgagcctgt ctacaagctc agcctcaaca gcgaccgctt cgtctcggat
     2401 ccggacacgc cgcttgacga tggcgatgtc ctcttgctgg tgtcggccga cgcgggaggc
     2461 tgactggatg gtgccgggtt accacggacg ttaccttcgc gttgatccgc ggcccggccg
     2521 caccgggtgc gtggcactcg aagagcgagt ccttcgacat tttcttggtg gggtcggact
     2581 gggtgcctgg atcctggccc atgagacctc tcccggtgtt gacccactgg gacccgaggc
     2641 ggccctggtg tttgcgctga gcccgctggt cggcagcccg ctcaccacgt cggccaaatt
     2701 cgcggtcgtg gcctttagcc cgctgaccgg ccgagtctgc gacgctctat catcgtctca
     2761 cttcgcgatc gccgccaaga ggacgggtgt cgattcgctc gtggttacgg gctcgtgcga
     2821 cgtgccgtca atcgtcttga tcgacggcac cgaccgggtc gagccgaccg tccagtggtt
     2881 gccggccagc tcgctctggg gcttatccgc gcaggaagcc gaggaacgca ttcgctcgca
     2941 acatggccgc gaatggcaag ttgcggcgat tggtccggcg ggcgaacggc tgataccgtt
     3001 tgctaccttg agccatgacg gccgtcacgc cggccggggc ggtttggggg ccgtactggg
     3061 ctccaagcgt atcaaggcga ttgccgtgcg tggcaaccac ctatcgcagc cggccgaccc
     3121 ggcgcaaacg atcgcacttg caaaaaaata tgcacggaag tcattcggac cggcgaccga
     3181 aaagtaccga gaattgggaa ctgtcgcgaa tctgctggtt ttcaaccggt tcgatgcgct
     3241 tcccacgcgc aattttcagg caagctcgtt cgagggtgcc gacctcttga gcgcggccga
     3301 gttggcaccg gcccggcgga ttgcgcgggc ttcgtgtgcg tcgtgcacga tcggctgcga
     3361 gcacatctac gcggcgggat cgggcgatag tggcggcgtt cggctcgagt atgagtcgct
     3421 ctatgcattg gggccgctgt gcggggtcga tcagccggct gcggtcctgg ctgccgccaa
     3481 ggcttgcgac gacgccggta ttgacacgat cagcaccggg gcgactatcg cgttcttgat
     3541 gcaatgtgcc gagaggggcc tggtcggcga gcggctcgag ccttcgcagc gaccgctccg
     3601 gtttggcgat ggtgtggctg tgcgcgaagc aattgatgcc ctggtggcgc ggcggggaac
     3661 gttgggcgag ctgttggcgc tggggagccg gcg
//
LOCUS       DATAQL010001230         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_735932, whole genome shotgun sequence.
ACCESSION   DATAQL010001230 DATAQL010000000
VERSION     DATAQL010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735932"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VHS97_21260"
     CDS             complement(<1..492)
                     /locus_tag="VHS97_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450796.1"
                     /translation="MSVISTARPARILINPVGWLGRSVLAVVSYSGGVTLLAFGALGS
                     LLWPRRDRAQAAAAPGFWKTLMHQLFWMLMMGVPLVGMVHVAMGSFLSLQAYYGSTFI
                     DGTGAVVGVGLLRNLGGLMTGLTFAGILSARMVPELHILSRRLLVAEMPAVPSGLGGR
                     RPPE"
     gene            complement(515..943)
                     /locus_tag="VHS97_21265"
     CDS             complement(515..943)
                     /locus_tag="VHS97_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450797.1"
                     /translation="MRIGRWIGKVAFIAATAYVCGCAHGTRDVRKVRTATPLARARAV
                     GRPNGRSDSGVMRTLVTRLGDVDPVVRLAAHEELRKRTGVDFGYVPWASEEERALPME
                     RWRAWVEQGNASQQPVARSIVAPSPPGKVLPAVNPQVTGP"
     gene            complement(1125..>3831)
                     /locus_tag="VHS97_21270"
     CDS             complement(1125..>3831)
                     /locus_tag="VHS97_21270"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX3450798.1"
                     /translation="LKGSFSGLGKLVLPWLFDRTVVVNPSEVDFVVVRPTGARLDLKT
                     VDATVEVKQSSRVVTRLRRSIAIEGGPAIPPDVASTPLIPSLLPQPSQSVEDRLDSGG
                     VLDAGDDAARGAGKAIRPPSVAMGEARLGDAPPSAAGSEVRRLLGHTNEVFDLVLSRD
                     GRFLVTCDNDDVIRVWDPASGTNLHTLRGHRGGVAVLAMSPNGRHVLSGGADHVLRLW
                     DIERGQLLREFRGQGDTIMAIAFTPDGRRCISAGGGRPHAQVGTDLDIWVRDVEDGRV
                     IARWPGHTGVIDALAVSPDGKLALSSSSDQTSRLWDLATGRELRRFPGQPELDLHAIF
                     SPDGRRAIVTSAGHLIRIFDVESGRELLQLRGHIAKVDSLAASPDGRLLVSGSWGERM
                     FRIWDLTDGRALGQIALEGNPQLGTFTPDGRRIFWSFSDKTVREFALADSVLSIRQSV
                     AAGPSEPLVRKLDGKISDVVIGGGGRYLILTLKDARRLAIFDVNAADFVKTIALPTEN
                     ALVSAGAKALFIAFPEQGLFQRWDLETMARQGENLPSPIQARLTGLAMGSDSEGPLLA
                     VWSHDSSNGIPQSARFSFLDSKTLKVLKAGPIVTGGFQGIGSVSLSGGSFLLHPMLQE
                     RVHVRASAGGELFGIWQTKGSPSGFQTLAVHRASLRAIYNHDAFDHLAPGPDGLTVYT
                     GRGGPLNAEGKPVRASDSRPPTSSELTVPSSDPAYYLSVRGLSDNRSPNQPAGAVTVS
                     VHEANDGTRLLTVRDLSEMIGTGNNEAWIPDDFTVEKRFHLVPAARLLITIPPTNDQL
                     VLRRLDIDKALQQLGVDALTVITPSNLRAKAGKLLDHQIEARSKSGGIRYTRAKGPEG
                     LTVSPTGKLTWTPPKPLSGDTIKAVVIVADSSGREHFHTITIRVD"
BASE COUNT          725 a         1228 c         1236 g          642 t
ORIGIN      
        1 ctccggcggg cgtcgccccc ccaagcctga tgggaccgcc ggcatctcgg cgacgaggag
       61 gcgccgtgag aggatatgca gctcgggaac catccgcgcg gacagaatgc ccgcgaaggt
      121 gagcccggtc atgagccccc ccaggtttcg cagtaatcct acaccgacga cagcgcccgt
      181 accgtcgata aacgtgctgc cgtagtatgc ctgcagcgac aaaaatgacc ccatggcgac
      241 gtgcaccatc cccacgagcg gcacacccat catgagcatc cagaaaagct ggtgcatcaa
      301 tgtcttccag aagccgggcg ccgcggcggc ctgcgcgcgg tctcgccggg gccatagcaa
      361 ggagcccaga gccccgaaag ccagcagagt cacgcccccc gagtacgaaa cgaccgcgag
      421 caccgaccgt cccagccaac ccacagggtt gatcagaatg cgcgcaggtc gagcggtcga
      481 aattacactc atcgcgagaa tcctccgcag tctctcatgg acctgtcacc tgagggttca
      541 ccgccggcaa aacctttccg ggtggactgg gagcaacgat tgaccgagcc actggctgtt
      601 gacttgcgtt gccctgttcc acccaggccc tccagcgctc catcggtagg gcccgttcct
      661 cctcacttgc ccacggaaca tagccgaagt cgacaccggt acgcttgcgt agctcttcgt
      721 gggccgccaa cctgacgacc ggatcaacgt cccccagacg agtcaccagc gtccgcatga
      781 ccccggaatc ggaccggcca ttcggccgcc caaccgctct tgctcgtgcc aagggagtag
      841 cagtccggac cttgcgaaca tcgcgcgtgc cgtgagcgca cccgcagacg taggccgtcg
      901 cagcgatgaa cgccactttc ccgatccatc ggccgatgcg cacaacccct tccttggatc
      961 gtcgcgccgt cgccagacta cctggcgcac caagcgcgcc gacgtcctgt cgtcaaaact
     1021 cgccgcgaac tctagtgaaa gtcggcaaat gagacaatcc caatcgtgaa ctcgaagaat
     1081 tgaggaaacc ggtgaagccc gccgttgcgg ctggccggac tgctttagtc gacgcggatc
     1141 gtgatcgtgt ggaaatgctc tcttcccgag gagtcggcga cgatgacgac cgccttaatg
     1201 gtatcaccgc tcagcggctt cggaggcgtc caggtgagct tgcccgtcgg ggagacggtc
     1261 aggccttccg gacccttggc gcgagtgtat cgaatgccgc ccgacttgga acgggcttcg
     1321 atttgatggt ccagcagttt gccagcctta gcgcgcaggt tcgagggcgt tatgacggtc
     1381 aaggcgtcga ctccgagctg ctggagggct ttgtcgatgt cgagcctgcg caacacgagt
     1441 tggtcgttgg tcggtggtat ggtgatcagc agcctcgcgg ccggtacaag atggaaccgt
     1501 ttctcgacag tgaaatcgtc tggaatccat gcctcattgt tccccgtgcc gatcatctcg
     1561 ctgagatcgc gcactgtcaa aagtcgcgtg ccatcattgg cttcatggac cgagacggtg
     1621 actgcgcccg ccggctgatt cggcgacctg ttgtcactca gcccgcggac actcaggtaa
     1681 tacgcggggt cagacgaggg aacggtcaac tcggacgatg ttggcgggcg ggagtcggac
     1741 gccctcacag gtttcccctc cgcgttgagc ggtccgccgc ggcctgtgta gaccgtgaga
     1801 ccgtcgggtc cgggggcgag atggtcgaat gcatcatggt tgtagatcgc cctgagcgac
     1861 gcgcgatgca cggccagggt ctgaaatccg gatggcgaac ccttcgtttg ccagattccg
     1921 aagagttcac cgccggctga ggcgcggacg tggacccgct cctggagcat cgggtggagc
     1981 aggaaactac cgcccgagag agagacgcta cctatcccct ggaagccacc ggtcacgatc
     2041 ggcccggcct tgagcacctt cagggtcttg gagtcgagga agctgaaccg ggcactttgc
     2101 gggatgccat tgctcgaatc gtgagaccag accgccagta gcgggccctc agagtcgctg
     2161 cccatggcca ggcccgtcag tcgtgcctgg atcggagatg ggagattctc gccctggcgg
     2221 gccatcgtct cgagatccca gcgctggaag agaccttgct cggggaaagc gatgaagagc
     2281 gccttcgcgc ctgcactgac gagggcattc tcggttggga gtgcgatcgt cttgacgaag
     2341 tcggccgcgt tgacatcgaa gatggccagc ctgcgcgcgt ccttgagggt cagaatgagg
     2401 taacgtcccc caccgccgat gaccacatca cttatcttgc cgtcgagctt acggaccaat
     2461 ggttcactcg ggccagcggc cacggattgc cgaatcgaaa ggacggaatc cgcgagtgca
     2521 aattcgcgca ccgtcttatc ggagaaagac cagaagattc gtcgcccatc gggcgtgaag
     2581 gtcccgagct gggggttacc ttccagggcg atctgtccga gcgcgcggcc gtcggtcaag
     2641 tcccagatgc gaaacattcg ttcgccccag cttccagaga cgaggaggcg tccgtcgggt
     2701 gaggccgcga gcgagtcgac cttggcgata tggcctcgca attgaagaag ctcccggccg
     2761 ctttcgacgt cgaagatgcg gatcagatga cccgccgagg tcacgatcgc gcggcggccg
     2821 tccggcgaga agatggcgtg gagatcgagc tctggctggc ccgggaatcg acggagttct
     2881 cggcctgtgg ccaggtccca cagccgagag gtctggtcgg aggaagacga aagtgcgagc
     2941 ttgccgtcgg gagagacggc gagcgcgtcg atgacaccgg tgtgtcccgg ccagcgggcg
     3001 atgacccggc cgtcctccac gtcgcggacc cagatgtcta gatccgtccc gacctgggcg
     3061 tgcggccgcc caccgccggc ggagatgcag cggcggccgt cgggagtaaa ggctattgcc
     3121 atgatggtat cgccttggcc tcggaactcg cggaggagct gccctcgctc gatgtcccac
     3181 agccggagca catggtccgc cccgccggag aggacgtggc gaccgttggg ggacatggcc
     3241 aggaccgcca cgccgccgcg gtgacccctc agcgtgtgca ggttcgtacc gctggccgga
     3301 tcccagacgc ggatgacgtc gtcgttatcg caggtgacga gaaaccgccc gtcacgggaa
     3361 aggaccagat cgaagacttc attcgtatgg ccgagcagtc tccggacctc gcttcccgcc
     3421 gcggacggcg gggcatcgcc gaggcgagcc tcgcccatgg cgaccgacgg cgggcggatg
     3481 gccttgccgg cgccgcgagc cgcgtcgtcc ccggcgtcga gcacgccgcc ggagtcgaga
     3541 cgatcctcga ccgactggga cggctgtggc agcaagctgg gaatcagcgg tgtcgacgcg
     3601 acgtcgggag ggatagccgg tcctccctca atggcgatcg agcgtcgcaa ccgagtgacg
     3661 accctgctgc tctgcttcac ctcgacggtc gcgtcgaccg tcttcaggtc gagccgtgcc
     3721 cccgtcgggc gtaccacgac gaaatccacc tcggacgggt ttacgacgac ggtgcggtcg
     3781 aacaaccacg gcaaaacgag cttgcccagg ccggagaatg accccttcaa t
//
LOCUS       DATAQL010001231         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_735990, whole genome shotgun sequence.
ACCESSION   DATAQL010001231 DATAQL010000000
VERSION     DATAQL010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735990"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VHS97_21275"
     CDS             complement(<1..320)
                     /locus_tag="VHS97_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450799.1"
                     /translation="MGLITASLVFPMYSNCTDVAAGEGLTAAGNVRERLAPRHIRADS
                     AAAATGAERHEFRQDFVSYEAHRPSEMCGADCRGWYTLACQSAANQRAIPGRRVLSSR
                     SPAFA"
     gene            514..1542
                     /locus_tag="VHS97_21280"
     CDS             514..1542
                     /locus_tag="VHS97_21280"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3450800.1"
                     /translation="MRYLFFSAAVSLVTLSGQTPKQTPLARLVDAELSRFPAKTGLYV
                     KHLTTGEELAVRGDDHFNSASVIKIPVMILAYRMADQKKLDLAERYTIKPSDFRGGSG
                     IFRYHDAGLNPTLRDIITEMIITSDNTATDIMIAKVGGKEKVNEFLKQNGIATSFLVQ
                     TVFELFRKPYEVLDPENKSLTPEDVFAMQSNVPAFTEPRKDLIKRLREGMREKNIQRE
                     MMKLYAEDEKYWLGVVTPTEIGRLLEGIEKGTFASKASSAEMVRIMRGQQSGARRIPH
                     YLNVPVGHKTGDFPPIVANDVGMIYAKSGTIIMSFFTLGNTGVYAETEDRMGQLARLI
                     VEYFDGPN"
BASE COUNT          385 a          522 c          475 g          339 t
ORIGIN      
        1 gcgaatgccg gagacctgga tgacaggacg cggcgtcccg gaatcgcccg ttgattggcc
       61 gccgactgac atgcaagagt gtaccatccc cggcagtcgg cgccgcacat ttccgaagga
      121 cgatgcgctt cataagaaac gaaatcctgc cgaaactcat ggcgctcggc tccggtcgcg
      181 gccgccgccg aatccgcgcg aatgtgccgc ggagctaaac gttcgcgtac gttccctgcc
      241 gcggtcagac cctccccagc ggcgacgtcg gtgcagttcg aatacatcgg gaagacaagg
      301 cttgccgtga tcagtcccat caccggtgcg cgttatcaat tcgattcgcc gggcggtcgc
      361 ctggccgtcg actcgcgaga tcaatcgatg atgatttacg ttccggatct tcggccggtg
      421 cgtttcattc gaactcccta accacgtcgc gctggctaac tgagtgtggg atcatccaaa
      481 tggaaatcct ttcaaagggg gcacgaaact cccatgcgct atcttttctt ctccgccgcc
      541 gtgagcctgg taaccctgtc agggcagact cctaaacaga caccgctcgc acgcctggtc
      601 gacgccgagc tctctcgctt tcccgccaaa acagggctct atgttaagca tctgacgaca
      661 ggcgaggaac tcgccgtgcg tggcgacgac cacttcaata gcgcgagtgt catcaagatc
      721 ccggtcatga tcctggctta ccggatggcc gaccagaaga agcttgatct ggccgagcgg
      781 tacaccatta agccctccga cttccgtggc ggctccggca tcttccgtta tcacgacgcc
      841 ggattgaatc cgacgttgcg ggacatcatc accgagatga taatcaccag cgacaacacg
      901 gccaccgaca ttatgatcgc caaggtcggc ggcaaggaga aagtgaacga atttctgaag
      961 cagaacggca tcgccacttc gttcctggtg caaaccgtct tcgaactttt ccgcaagcca
     1021 tacgaggtgc tggatcctga gaataagtcg cttactcccg aagacgtatt cgccatgcag
     1081 agtaatgtgc ccgcgttcac tgagccgcgc aaagacctca tcaagcggct ccgcgagggg
     1141 atgagagaga aaaatatcca gcgggagatg atgaagctct acgcggagga tgagaagtac
     1201 tggctcggtg tcgtcactcc cactgagatc ggccgcctgc tggaaggaat tgaaaaagga
     1261 acctttgcct cgaaggcatc gtcggcggaa atggtccgca tcatgcgagg gcagcaatcg
     1321 ggcgcccgcc gcatccctca ttatctgaac gtcccggttg gtcacaagac gggcgacttc
     1381 cctccgattg tggccaacga tgtgggcatg atctatgcga aatccggcac catcatcatg
     1441 tcgttcttca cgcttggcaa taccggtgtt tatgcggaaa cagaagatcg aatgggccag
     1501 ttggcccgcc tgatcgtgga gtacttcgac ggccccaact aaactcattt ccgctgattg
     1561 cccggactat acctgctcgt cgaattcggc ctcgcgctcc ggtaggacat cgtgcgtacc
     1621 cgccatccac gcgtacagcg tgggcagcag gaaaatactc atcaccagat cggagatcaa
     1681 tccgcccaca atcacgatcg cgaacgggcg ctgcgaatcc g
//
LOCUS       DATAQL010001232         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3026485, whole genome shotgun sequence.
ACCESSION   DATAQL010001232 DATAQL010000000
VERSION     DATAQL010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026485"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1044)
                     /locus_tag="VHS97_21285"
     CDS             complement(118..1044)
                     /locus_tag="VHS97_21285"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3450801.1"
                     /translation="MLIEPTLRSDLLADRVAIVTGGSRGIGGATATMLAANGAHIVVA
                     DVDASKAEETVDSLNHDFGDDVASAFVADLVAPNACDALVQHALGIYGHLDIVVNNAG
                     YAWDGGLHNMSDEQFQAMLDVHLVVPFRLARATAPIFRAAAQADDQHGDASRHRKTVM
                     VSSLAGQWGLVGAGNYAAAKAGMLGLMRTMAQEWGQFRVNVNAVAFGSVQTRFGLPQS
                     DREVIETGGRTIRVGMQAKHAERRGVHVDPDHVPTDAEIYAAKPLPVVPLGRSGTIRE
                     AADTIFWLCSPLSDYVTGQVIAVSGGARGGMS"
     gene            complement(1044..1826)
                     /locus_tag="VHS97_21290"
     CDS             complement(1044..1826)
                     /locus_tag="VHS97_21290"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX3450802.1"
                     /translation="MTEKEVVHVELVDHVLTITMDRPEARNALNYQMMSELAAAFERL
                     DNELDCWVGVLQGEGPAFCAGADLKEALQARANHKEPTRPPNAASIFRPHRKPLIGCV
                     EGYAFAGGLEILMTCDLVVATTEATFALSEVKRGLLAIGGGLFRLPRRLPTNIAKELI
                     ITGAAMPATVLHQYGLINRLTQPGDARSAARDLATAIAANSPVAVQAALAVVGSSVAE
                     GWTDDQGWAGQKGPSQRVLASEDLLEGLRAFSEKRAPNWSGR"
     gene            complement(1851..2498)
                     /locus_tag="VHS97_21295"
     CDS             complement(1851..2498)
                     /locus_tag="VHS97_21295"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3450803.1"
                     /translation="MSPSELALPLRRTQAERRQETEQALLAAATRLFARIGIDNASLA
                     DIGEEAGYSRGLVNHRFGSKAAFVERLAGDSQTAFIDALDQSHPSGEIATLFQVIDTY
                     LDRVSEESTASRSFFVMWGAALPSSSALRPIFMADDARFRNGIEAIIRTGQAKGRIES
                     TVDAKSFAVAFVGLLRGVGAQYMIDPEAVDLAPCKTTIVGFVESVLTTSPPNPST"
     gene            complement(2485..>2989)
                     /locus_tag="VHS97_21300"
     CDS             complement(2485..>2989)
                     /locus_tag="VHS97_21300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3450804.1"
                     /translation="NAGANPKGLTGKPFWEKNPSEWEPFLRVNLYGPLHTCRAVIPGM
                     IEAGNGGRLITVISEAGRTGEVGLEAYSAAKAGAAGLTRALARGLGRYEITANTVAIA
                     ATRTPATAARMDDEDRAKKVLAKYMIRRFGQPSDVAAMITFLASPASSWITGQTYPVN
                     GGFSVTL"
BASE COUNT          551 a          917 c          937 g          584 t
ORIGIN      
        1 ccgcgatgtg ccagcgaaaa tcgggatgga tcagccggtc gaatcggtca gtatcacggt
       61 tattccaggc ggccacaaac tcctgggcca acacctccat tccgagttcg ctactcatca
      121 gctcattcct ccccgggcac cgccgctgac ggcgatgacc tgtccggtga cgtagtcaga
      181 tagcggagag cagagccaga aaatcgtgtc ggcggcttcg cgaatggtcc cgcttcggcc
      241 gagcgggacc accggcaacg gcttcgccgc gtagatctcc gcgtcggttg ggacatgatc
      301 tgggtcgacg tgtacacctc ggcgctctgc gtgctttgcc tgcattccga cgcggattgt
      361 tcgccccccc gtttcgatga cttctcggtc cgattgaggt aatccgaaac gggtctggac
      421 actgccgaac gcgaccgcat tcacgttgac gcggaactgt ccccactctt gggccatggt
      481 ccgcatcagg ccgagcatgc cggccttggc ggccgcatag ttgccagccc caaccaggcc
      541 ccactgacca gcgagcgacg agaccatcac ggtcttgcgg tgacgggatg cgtcaccgtg
      601 ctgatcgtca gcctgggcag cggctctaaa gataggagcg gtggcacgag ccagacggaa
      661 cgggaccacc agatggacgt cgagcatggc ttggaactgc tcgtcgctca tgttgtgcaa
      721 tccgccgtcc caggcatagc cggcgttatt caccacgatg tcgagatggc cgtagatccc
      781 aagggcgtgc tgcacaaggg cgtcgcacgc gttgggagcg acaaggtcgg cgacgaatgc
      841 actggccaca tcgtcgccaa aatcatggtt cagtgagtcc acagtctctt cggccttcga
      901 ggcatcgacg tccgcgacga cgatgtgtgc cccgttcgca gcgagcatcg ttgcagtagc
      961 acctccgata ccgcgtgatc cgccagtaac aatggcaacg cgatccgcga gcagatcgga
     1021 gcggagtgtg ggctctatga gcatcagcgg ccgctccagt tgggcgcacg cttctccgag
     1081 aaggcacgga gaccttcaag cagatcctcg gaagccagca cccgctggga tggccccttt
     1141 tgccccgccc aaccttggtc atcggtccat ccttcggcaa cggagctacc gaccaccgca
     1201 agggcggctt gcacggccac tgggctgttg gccgcgatgg ctgtcgcgag atcgcgagcg
     1261 gcggaccggg cgtctccggg ctgagtgagg cggttgatga gcccgtattg atggaggacc
     1321 gtggcgggca tggctgctcc ggtgataatc agctccttgg cgatattggt gggcaggcgt
     1381 cgagggaggc ggaacagtcc gcctccgatg gcgagcaacc ctcgcttcac ttctgacagg
     1441 gcgaacgttg cttccgttgt cgccactaca aggtcgcacg tcatgaggat ctcgaggccg
     1501 ccggcaaagg catatccctc gacgcagcca atgagtggtt tgcggtgagg acggaagatc
     1561 gacgccgcat tgggcggtcg cgtgggttcc ttgtggttcg cacgggcctg caaagcctct
     1621 ttgaggtcgg caccggcgca gaaggcggga ccttcgcctt ggaggactcc cacccagcag
     1681 tcgagctcgt tgtcgagccg ttcgaatgca gccgcgagct ctgacatcat ctgataattg
     1741 agcgcgttgc gagcttcggg ccggtccatc gtaatagtaa gtacgtgatc gacgagttcg
     1801 acgtgcacga cctctttctc agtcatgggc ggtggtcctt tctgacgcca ctacgtcgag
     1861 gggttgggcg gactcgtcgt cagcacactt tctacgaagc caacaatcgt tgtcttacag
     1921 ggcgcgaggt caaccgcttc ggggtcgatc atgtactgag cgccgacacc ccggagaagg
     1981 cctacgaatg ccacggcaaa gctcttagcg tcgaccgttg attcgatgcg gcccttagct
     2041 tgaccggttc tgataatggc ttcgattcca ttcctgaatc gggcgtcatc agccatgaag
     2101 atcggccgca gagcgctact gcttggaagc gccgcacccc acatgacgaa aaaggatctt
     2161 gacgcggtcg attcttcgct cacacgatcg aggtacgtgt cgatcacctg gaaaagcgtt
     2221 gcaatctcac cgctgggatg tgactgatcc aaggcatcaa tgaaggccgt ctgcgagtcg
     2281 cccgccaggc gttcgacaaa cgccgccttc gagccgaagc gatgattcac taaaccccgg
     2341 ctgtagccgg cctcctcacc gatgtcggcc agcgacgcgt tgtcgattcc gatccgggca
     2401 aagagccgag tcgcggcggc cagtaacgcc tgttctgttt cttgccgacg ctccgcttgt
     2461 gtacgcctga gtggcagcgc gagctcagag ggtgacactg aatcctccgt tgacgggata
     2521 cgtctggccc gtaatccagc tcgaggcagg agaggcgaga aaggtgatca tggctgcgac
     2581 gtcgctgggc tgaccgaacc gacggatcat gtacttcgca aggaccttct tggcgcgatc
     2641 ttcgtcgtcc atgcgtgccg ccgtggcagg ggtgcgagtg gcggcgatgg ccacggtgtt
     2701 ggcggtgatt tcgtagcggc cgaggcctcg ggccagggca cgcgtcagcc ctgccgcgcc
     2761 ggccttggcc gccgagtacg cctcaagccc gacctcacca gtgcgtcccg cttcgctgat
     2821 gaccgtgatg aggcgtccgc cgttgccagc ctcgatcatg cctggaatca ccgcccgaca
     2881 ggtatgcagg ggcccataga ggttgaccct gagaaacggc tcccattcgg atgggttctt
     2941 ctcccagaat ggtttgccgg tgagcccctt cggatttgcc cctgcgttt
//
LOCUS       DATAQL010001233         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_124584, whole genome shotgun sequence.
ACCESSION   DATAQL010001233 DATAQL010000000
VERSION     DATAQL010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124584"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..330)
                     /locus_tag="VHS97_21305"
     CDS             complement(1..330)
                     /locus_tag="VHS97_21305"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3450805.1"
                     /translation="MSSGGLSKARLSRLHDVMAGYVERGELPGIVSPVSRRGEVHVDA
                     VGMQAIGGKIPMRRDTIFRIASMTKPITAAAAMILVEECKLRLDEPVDRLLPELAGRK
                     VLSHFDD"
     gene            complement(494..814)
                     /locus_tag="VHS97_21310"
     CDS             complement(494..814)
                     /locus_tag="VHS97_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450806.1"
                     /translation="MNACRCCEAAPTLARRCLGVTGWVIPSAILALLPKCPACLAAYV
                     AIGTGIGISMPTAAYLRSLLLVVCVASLLYLAARSVQLHRAHIQDEDNGILRRLVSDQ
                     HDEN"
     gene            complement(811..1647)
                     /locus_tag="VHS97_21315"
     CDS             complement(811..1647)
                     /locus_tag="VHS97_21315"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX3450807.1"
                     /translation="MITNTITHPKIVSRDEWLRERKELLAKEKELTRRRDALNAERRR
                     LPMVRIDKEYVFEGPDGKASLLDLFDGRRQLIVYHFMFDPGAPPPGKSGEPWDEGCSG
                     CSLTADNIGHLAHLHARDTSLVLVSRARLDKIEPFKASMGWTVPWFSSFGSDFNYDFH
                     VTLDEAVAPVEYNYKDKATLEKNGENWFLHGEVPGVSVFLRVGDSIFHAYSAYSRGIE
                     PLDGTVSYLDMTPYGRQEDWEDSPEGWPQTPTYGWTRHHDKYNALPKDSDSCCNSRET
                     HA"
     gene            complement(1714..2070)
                     /locus_tag="VHS97_21320"
     CDS             complement(1714..2070)
                     /locus_tag="VHS97_21320"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3450808.1"
                     /translation="MKYLCLVYIDEKALDALSKSDYGALDSESLAYDEELRKSGHFIV
                     AQALEPVQAAKTLRPQKGKISITDGPFAETKEQLGGFILIDAKDLNDAIQVASKIPVA
                     RLGCIEVRPIKELNPQ"
     gene            complement(2067..2537)
                     /locus_tag="VHS97_21325"
     CDS             complement(2067..2537)
                     /locus_tag="VHS97_21325"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3450809.1"
                     /translation="MCLVYQDEKKLDALSQDEMDAVVGACIGWVDQLEKSGHHIFSAG
                     LQCVRTATTVRHRDGKVSQTDGPFAETKEFLGGFTLINARDKKEAIHLASTFPAAHLG
                     SMEVRPVLEGDGELTDALDKKIGAAFRRNSAQGIDPTIASRFVSIPRPNPAREP"
BASE COUNT          548 a          869 c          851 g          627 t
ORIGIN      
        1 tcaatcatcg aaatgactca ggactttgcg gccggcgagc tcgggcaaca gccgatcgac
       61 cggctcgtcc agccgcagtt tgcattcctc gacgaggatc atcgccgccg cggccgtgat
      121 cggcttggtc atcgaagcga tgcggaaaat tgtgtcgcgc cgcatcggaa ttttgccacc
      181 gatcgcctgc atgccaaccg catcgacatg cacctcgccc ctacggctaa ccggcgagac
      241 gattccgggt aattcgccac gctcgacata gccggccatg acgtcgtgca agcggctgag
      301 ccgcgccttg gacaatccac cgctactcat cgcttgttcc ccctgtctcg tatcatccga
      361 gcttgatggc cgacttggtg cggcctcccg cgaatgcgcg gccccggttg ccgacgcgac
      421 cagcgccgcg gaagtgccag tcgtcttcaa aaacgtccga cggtcgagtg cagaaatgtc
      481 gctattgcta atattagttt tcgtcgtgtt ggtcactgac caatctcctc aagataccgt
      541 tgtcttcgtc ttgaatatga gcgcgatgaa gttgcacgct tcgtgcggcc aggtataaca
      601 gtgacgcgac gcacacgacc aacaaaagag accgcaagta cgccgcggtt ggcatcgaga
      661 ttccaatccc ggttccaatc gcaacgtagg cagccagaca tgcgggacac ttcggcagga
      721 gcgccaggat cgcgctcggg atcacccatc ccgtgacgcc cagacagcgt cgcgccaatg
      781 tcggcgcggc ttcgcagcag cgacaagcgt tcacgcgtga gtctcccgcg aattgcagca
      841 ggagtcagag tcttttggaa gggcattata cttgtcgtgg tgtcgggtcc acccgtacgt
      901 cggagtttgc ggccaaccct ccggtgaatc ttcccagtct tcttggcggc cgtagggcgt
      961 catgtcaaga tagctgacgg taccgtcgag cggctcgatc ccgcgcgagt aggccgagta
     1021 ggcgtgaaaa atgctgtcgc ccacgcgaag gaatacgctc acgccgggca cttcaccgtg
     1081 aaggaaccag ttctcaccat tcttctcgag cgtcgccttg tccttgtagt tgtactcgac
     1141 gggagcgacg gcttcgtcca gcgtgacatg gaagtcgtaa ttgaagtcgc tcccgaacga
     1201 ggaaaaccac ggaacggtcc agcccatgct cgccttgaag ggctcgattt tgtccagtcg
     1261 tgcgcgggag acgaggacga gggaagtgtc gcgagcgtga agatgcgcga gatggccgat
     1321 gttgtcggcc gtaagcgagc aacccgagca gccttcgtcc cagggttctc cggacttccc
     1381 cgggggtggt gcacccgggt cgaacatgaa gtggtacacg atgagctgac ggcggccatc
     1441 gaacagatcg agcaggctcg ccttgccgtc cgggccttca aagacatact ccttgtcgat
     1501 cctgaccatc ggcagcctgc gtcgttccgc gttcagcgcg tctcggcgac gggtgagttc
     1561 tttctccttt gcgagcaatt ccttgcgctc acgaagccat tcgtctcgcg agacgatttt
     1621 cggatgtgtg attgtgttcg ttatcatgac gtgttctcct ttgactctga ttgggcgttg
     1681 tcgtcggcag atctgttgtg ggtccggatg cggttactgc ggattcagct ccttgatcgg
     1741 tcgcacctcg atgcagccca ggcgcgccac cgggatcttc gacgccacct ggatggcatc
     1801 gttcagatcc ttggcgtcga tcaggataaa accgcctaac tgctctttgg tctcggcgaa
     1861 gggaccgtcg gttatggaga tcttgccctt ctgtggccgc aacgtctttg cggcctgaac
     1921 cggttcaagg gcctgcgcga caatgaagtg accgctcttg cggagctcct cgtcgtaggc
     1981 gagggactcg gaatcgagtg cgccgtaatc gctcttggac aaggcgtcga gcgccttctc
     2041 gtcgatgtag accaggcaca gatatttcat ggttccctcg cgggattggg tcgcgggatc
     2101 gatacgaatc gagaggcgat ggttgggtca attccctgcg cgctgttgcg gcggaatgca
     2161 gcaccgatct tcttatctag cgcgtcggtc agctcgccgt ccccttcaag gactggtcgc
     2221 acctccatgc tccccaggtg cgcagccgga aacgtcgagg ccagatggat ggcctctttc
     2281 ttgtccctgg cattaatcag ggtgaacccg cccaggaact cctttgtctc ggcgaaggga
     2341 ccgtcggtct gggacacctt gccgtcccgg tgcctcacgg tcgtggccgt ccgcacgcat
     2401 tgaaggcccg cggaaaaaat gtggtggccg ctcttctcga gctgatcaac ccagccgatg
     2461 caggcaccca cgaccgcgtc catctcgtcc tgagacaggg cgtcgagctt cttttcgtct
     2521 tggtacacca agcacatgta ctgcattggt tcctccgaat cgtggtgaca ttccagtgga
     2581 catattcaag tcgattggtg cagtccaaaa tcgacaactg cagactaccg acgaccgatt
     2641 ttcgtttctc gcacggccgg cggacgcgca acttcgaaca gtgcatggga tttagttttc
     2701 aaccgatgga tcccatcccg gcttctcagc acgcatgccg tcaatccgag gtgtatagag
     2761 cttctggcca gggtttgcga tcagcgcaca atgaagcccg cgttcgccac tgcatcgcga
     2821 accgcgggcc gttgcaagcg agtgctgtca agaattgtgg accaggcgtt cggtttccca
     2881 aaccggcggc tcagc
//
LOCUS       DATAQL010001234         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3332324, whole genome shotgun sequence.
ACCESSION   DATAQL010001234 DATAQL010000000
VERSION     DATAQL010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3332324"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(484..>1629)
                     /locus_tag="VHS97_21330"
     CDS             complement(484..>1629)
                     /locus_tag="VHS97_21330"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3450810.1"
                     /translation="EGFTATPFITGLEHPRRLLVLPNNDVIVTEQKTGYLTLLRDQDG
                     DGKADYIERYADDFKAPYGLAYRDGFVLVADQEGIWRVPHVSGAVRAGRPDQPRITDV
                     PPEQRKPVPAAYGQELITQRGVFGVVAGHQNRHLALDPKTGAMFVGVGSAGNIGVEPE
                     VKASIQRFEADGSSQTTFASGMRNPTTLAFHPTTGELYAGVQERDGLGDNLPPDYLTR
                     VEKGAFYGWPYAYTGPNPQPGFAYLAPEKVKATVVPDVLFQPHSSVLDLVFYDGEQFP
                     AEYRGDAFVALKGSWNRSEPTGYKVVRVRFKDGRPEGSYENFVTGFWVSGQHRAEVWG
                     RPAALAIMKDGSLLIADDTGGTIWRVSYTGPRDRASDRKTTTGAMPR"
BASE COUNT          304 a          516 c          475 g          334 t
ORIGIN      
        1 tcgttggttt ccgtcggtcc tcaaggccat gaagatcatc cggcccgaga ccttggtgcg
       61 ctggcagagc aggaacggcg ggtcggaact tcggcctcat ctgacctgag tctaacctgg
      121 cagctctgct gcctgtcata atagagagtg tcgcggactt tccaccgtcc tgcatcaccg
      181 tgcgatatta actcgcccgg gagaggatct gtgctcgccg tgtgcagtgc aaggcggggt
      241 gatcaccccg ggccggtttc ggcaccggct tgatcaggca gccgccgact tagcggcgcg
      301 ctgctcctcg agcgcgcctt cgggcgacgc tggagcccgg aggatgcgac ctaccggcgc
      361 gcttgaatcg cgcaatctcc gcgtggcaga gctgacgccg atggccttgg ccgccgcccc
      421 gcgtccgtga tccttgcggc ggtcacttcc atcggccgtc tgccagcgac tcgcgctccc
      481 tcgctagcgc ggcatggccc ctgtcgtggt tttccgatca gatgcccggt cccttggtcc
      541 tgtgtaggac acgcgccaaa tcgtgccacc agtgtcgtcg gcgatcagca gcgacccgtc
      601 cttcattatt gccaaggcag cggggcgtcc ccacacctcg gcccgatgtt gaccggagac
      661 ccaaaagccc gttacgaaat tttcgtagct tccctccggt ctaccgtcct tgaatcgcac
      721 ccgaacaacc ttgtaaccgg tcggctcaga acggttccag gagcccttaa gcgctacgaa
      781 tgcatcccca cgatattctg ccggaaattg ttcgccgtcg tagaacacca ggtccaaaac
      841 cgacgagtgc ggttggaaca gcacgtctgg aactacggtc gctttcactt tttccggcgc
      901 cagatatgca aagcccggct gcgggttggg cccggtatat gcatagggcc agccgtaaaa
      961 ggcacctttt tcgacacggg tgaggtaatc gggcggaaga ttgtcgccaa gtccgtcgcg
     1021 ctcctgaacc cccgcgtaga gttcgccagt ggttggatgg aatgcgaggg tagtcggatt
     1081 gcgcattcca gaggcaaacg tagtttggct ggagccgtct gcctcgaacc gctgaattga
     1141 ggctttgacc tctggctcca cgccgatgtt gccagcggac ccgacgccaa caaacatagc
     1201 tccagttttt ggatccaacg cgaggtgacg gttttggtga cccgcaacta cgccaaacac
     1261 acctctctgg gtgatcaatt cctggccata ggccgccggc acaggcttgc gctgttcggg
     1321 cggcacatcc gtgatccttg gctgatctgg ccgaccggca cggacagcgc cgcttacatg
     1381 cggtacgcgc cagatgccct cctgatctgc aacgagcaca aagccatccc ggtacgcgag
     1441 gccataaggt gccttgaagt cgtcggcata gcgttcgatg taatccgcct tcccatcacc
     1501 atcctggtcc cgcagcagcg tcagatatcc ggtcttctgc tccgtaacaa tgacatcgtt
     1561 attggggaga acaagcaatc gacgaggatg ctccaggccc gtgataaacg gcgtggctgt
     1621 gaagccctc
//
LOCUS       DATAQL010001235         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_886428, whole genome shotgun sequence.
ACCESSION   DATAQL010001235 DATAQL010000000
VERSION     DATAQL010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886428"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VHS97_21335"
     CDS             complement(<1..448)
                     /locus_tag="VHS97_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX3450811.1"
                     /translation="MTSANRGIVFQQIDRLYREGTLAGLGDGQLLERYLTRRDEAAFR
                     ALVDLHGPMVLGLCRRVLRDPRDIEDAFHATFLVLIRKAPTIRDRGLLANWLYGVAYR
                     VARRARTHTLRRREREIAVGRVEISATAPAAEIEGIGPMLDQELNRL"
     gene            645..1508
                     /locus_tag="VHS97_21340"
     CDS             645..1508
                     /locus_tag="VHS97_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450812.1"
                     /translation="MTSYRVDPSTGSEGKPRPHWLKALGGEPLPETVAIDGVPHRLRE
                     TFKHDSWAATGLYEGSPGVLRVVKLHRHAPVGGFPMGWLGRTMARHEMHLLRSLADLK
                     GVPKLAGQVTVDGHQLANAVARDYIEGHPLGDREEVNQSFFPTLEAMLRAMHSRKIAY
                     VDLHKRENILVDEAGDPYLIDFQISVAWPRRLVAGPLFTLLRRGDEYHLMKHWARSRP
                     EQCGVDDVALRNRIPWMIRAHRLVARPFREMRRRLLVKLGVRTGKGRVESEFFAEHAL
                     RPASPNLRRPG"
     gene            1614..>2581
                     /locus_tag="VHS97_21345"
     CDS             1614..>2581
                     /locus_tag="VHS97_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEX3450813.1"
                     /translation="MERPIRSSLARFWPWLVLAMATIPSVWYVLDYEDDIDPELPAVV
                     RPTFNRYPPAAYRFAEAGDTIDHVAVYVASAALVLAAWGWFRNARSRGWAAATVVSAA
                     GFWHAATPGPLADGWYGLGWRIILDPRAPVANRLIMAGLTLGVVAMLLWALVDRPLRS
                     VWLAAKDRGVLGLSIAAALLIAARQVAWLDVEPVGFWPRWLYVWGMFAWSFVLVRLAP
                     AAPLGWSRNAIVAAMVLVSLGPDFAGRGLFWYQRPIHRLREVDPGRIYVSAMPTYQGL
                     KLAQERYHFRTIINLFPESTPEQSPHWPDELRFAREHGLKYVGNPAR"
BASE COUNT          457 a          802 c          798 g          524 t
ORIGIN      
        1 ggagtctgtt taactcttga tcgagcatgg gtccgatgcc ttcgatctcc gcggcagggg
       61 ctgtcgcgga aatctcgact cggccgacgg cgatctcgcg ttcacggcga cggagcgtgt
      121 gggttctggc gcgcctggcg actcggtaag ccaccccata aagccagttg gccaggagtc
      181 cacgatctcg gatcgtcgga gctttgcgga tcaggaccaa aaacgtggca tgaaaggcgt
      241 cttcaatgtc cctcgggtcg cgcaataccc tgcggcaaag gcccagcacc atgggaccat
      301 gcaggtcgac cagcgctcga aaggccgcct catcccgacg agtgagatac cgctcaagaa
      361 gctggccgtc accaagaccg gcgagtgttc cttcccgata aagccggtca atctgctgga
      421 acacgattcc gcgattggcg ctggtcatca aacaactccg atttcatggc tcgatcttct
      481 ctacttactg cagttgccgc cacaaccaat ccgttatttg gaaccaactg agcgaaatcc
      541 gcggaattcg gcaatcgatt caattgtcag gccgcggaga accgacaagt tgagaacgag
      601 tccgatcaca cgcacgttcc cccagccgat ggtcaggtgg caggatgaca tcctatcgag
      661 tcgatccgtc cacgggttcg gagggcaaac ctcgtcccca ctggctcaaa gcgctcgggg
      721 gtgagcctct gcccgaaacc gtcgccattg acggcgtgcc acaccgtttg cgcgagacgt
      781 tcaagcacga ttcctgggcg gccaccggtc tttatgaagg gtcgcccgga gtgttacgag
      841 tagtgaagct acaccggcat gccccggtgg gcggcttccc aatgggctgg ctaggtcgga
      901 cgatggctcg acatgaaatg catctactcc ggtcgttggc cgacctgaag ggcgtgccca
      961 aactggccgg tcaggtcacc gtcgatggcc atcaactcgc taacgccgtg gcacgggatt
     1021 acatcgaggg acacccgctc ggcgaccgcg aggaggtgaa ccaatcgttt tttcccaccc
     1081 tggaggccat gctccgcgca atgcattcgc ggaaaattgc ttatgtcgat ctacacaagc
     1141 gcgaaaacat tttggtggat gaggcgggtg atccctatct cattgatttc cagatcagtg
     1201 tggcctggcc gcgtcgactt gtcgcggggc cgctcttcac actcttgcgc cgcggcgatg
     1261 aatatcacct catgaagcac tgggcccggt cgcgtcccga gcaatgcggc gtcgatgacg
     1321 tggcgctgcg gaatcggatc ccttggatga tccgtgccca ccggctggtg gcaaggccat
     1381 ttcgcgagat gcggcgacgg ctcctggtga aactgggcgt gcgcacgggc aagggacgtg
     1441 ttgagtcaga attctttgct gaacacgcat tgcgccctgc gagcccaaac ctccgccggc
     1501 ctggctgatg gtctcctcct ggacgtcgag atggtgtaga gtaccgacag actcttccca
     1561 gcctggatgt cctggggatc ccgtcgtcga gtcggaaagg atgccgccga gacatggaac
     1621 gacccatccg atcgagcctg gcgcggtttt ggccctggct ggttcttgcc atggccacga
     1681 ttccatccgt ttggtacgtg ctggactatg aggacgatat cgaccccgaa ctaccggcgg
     1741 tcgtgcgacc cacgttcaat cgctatccgc ctgctgcata tcgctttgcg gaagctggcg
     1801 acacgatcga ccacgtcgcc gtgtacgtgg cgtcagcagc attggtactc gcggcttggg
     1861 gctggtttcg caacgcccgc agccggggct gggccgcggc gacggtggtc tccgcagcgg
     1921 gcttttggca cgccgccacg ccgggtccgc tcgccgacgg ctggtacggc ctgggatggc
     1981 ggatcattct cgatccgcgc gctcctgtcg cgaacaggct catcatggcc gggctgacct
     2041 tgggggtcgt ggcgatgttg ctatgggcct tggtcgaccg accgctgcga tcggtctggt
     2101 tggccgctaa agaccgtgga gttctggggt tgtcgatcgc ggcggcgctc ttgatcgccg
     2161 cgcgccaagt cgcgtggctg gacgtggaac ccgtcggctt ctggccgcgc tggctctacg
     2221 tctggggcat gtttgcctgg tcgtttgtcc tcgtcaggct cgcaccggcg gctcctctcg
     2281 gttggtcgcg caacgcgatc gtagccgcca tggtcctggt ttcacttggg cccgactttg
     2341 ccggccgcgg cctcttctgg tatcagcgtc cgattcatcg cctgcgggaa gtggatcctg
     2401 gccgcatcta cgtcagcgcg atgccaacct atcagggcct gaaactcgcg caggaacgct
     2461 accacttccg cacgatcatc aacctcttcc ccgagagtac tcccgagcaa agccctcact
     2521 ggcccgacga attgcgcttc gcgcgcgaac acggcctgaa gtatgtcggc aacccggcgc
     2581 g
//
LOCUS       DATAQL010001236         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1650199, whole genome shotgun sequence.
ACCESSION   DATAQL010001236 DATAQL010000000
VERSION     DATAQL010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650199"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..807)
                     /locus_tag="VHS97_21350"
     CDS             complement(133..807)
                     /locus_tag="VHS97_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEX3450814.1"
                     /translation="MRERWAGRLVVVLILGGVIAILAFNLNLKGRKQLGSASSKIELA
                     PVDPTPEQAQPQTGIARVREYPIGDEVEVNQMVIKAVWLPPVQMEGMVDSASSALIHL
                     EADIHATQGNRNGFAEHEFIPYLVVRYTITPQGGKTNGEAPKSIEGKLTPMVARDGLH
                     YGATIEMPRAGSFKLTYAIDPPSAGGLGRHVDPATGVDPWWKPFQVSFDWDYPGPPAS
                     PPSAVN"
     gene            complement(829..3174)
                     /locus_tag="VHS97_21355"
     CDS             complement(829..3174)
                     /locus_tag="VHS97_21355"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX3450815.1"
                     /translation="MRRARVIGLFQTCSLLWLLSCGLAAEVGAGTSVSGVVQMPDVCS
                     PAISPAAVYLTPSGSDQNGLAQRATSQPGKLPSAEVVLVNQRELQFMPRVRAIALGQT
                     VRFGNQDGETHNVHIVSPGFAFNQAMAPGQFQDFTPTQPGVMRLACDIHMHMRGYVIV
                     SPTRWAQVCGREGRFRLDDVSEGRYVLTAWHEMGDPVQTEITVTSDANSLVVPAIVLT
                     SSLGPTARRMDQKTARVRRWPDVIDRISVALAASRDAAARPNELAKARRLAEDAYWVE
                     FESSDMETAVRKYLGYVRAGELERQFHAIRTAVRDVAVKRIPREALAQSCHKLLHDLL
                     EVSNALDAKGITDPTRIDALGGQSGAEARPGVFPTELAPATGFAGDPRALLQALKSGL
                     RRVEEYAVRGESDDAASELTTVYMTEFEPLERYLMGRNPQAVRPLEMQFNRLRGDLAG
                     GLSGDKLSSRVNGLTNEVENLVDRLEARPVGTFGAAFVASLITILREGVEVILVVAML
                     LALVARATTGSVAPTHDEVKASAQTKVRASRAIWRGVGLAAVASLVTAVVLNVMVINA
                     QGAAREILEGVVMLAAAGVLFYVSYWLVSQLEAKRWMDFLKTQARHGLELGGQGTLAL
                     TAFLAVFREGAETSLMYQALLGSEGRTQSGFLGLIIGLGLGLILLAVVAALIRQTSVR
                     LPMHVFFKFSGLFLFALAIVFAGNGVFELQNAGILLTTNLAWMGRGLPWAGIYPNLQV
                     VSVQGMLLAGAVLAWVVVPRLSWWRAGGPAASGIGLAPKQG"
     gene            3446..4213
                     /locus_tag="VHS97_21360"
     CDS             3446..4213
                     /locus_tag="VHS97_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX3450816.1"
                     /translation="MEAEFPPAPLRPVYRSDYGGPVIVTTLEFGGKSIRLARPADPDR
                     LLDDPRVIDWNRSDDYMPYWAYLWPSALLLAEWVAREPWAERLDGAIAPEALEIGCGL
                     GLPGLVAVARGLRVRFTDHDPAPLEFVARSAAENRFDSGQYSIRPLDWRSPPDERFSI
                     ILGADVIYELRLVPLVAELLSRLLAPDGMGVITSPYRAAAQGFPAALAGLGLTCQEKS
                     VTARLPEGQTIEGTIYHVSRPNSAFTTGIFPGASPSI"
     gene            complement(4199..>4484)
                     /locus_tag="VHS97_21365"
     CDS             complement(4199..>4484)
                     /locus_tag="VHS97_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX3450817.1"
                     /translation="GLSGTAIKPVALRIVWEVARALPTLPIIGIGGIATADDALEFLV
                     AGASAIQVGTATFYEPTACERLLDDLTVQLEEIGVADIRSIIGTLQSNRG"
BASE COUNT          904 a         1416 c         1341 g          823 t
ORIGIN      
        1 aagaggcgat cgccctggcg attcactcca gccccgacag cctctttccc gagcccatgc
       61 tgttggggga tgcactctgc gaacgatttc gccggtacgt ccccttgatc gacatgtgaa
      121 cgctcagatg gatcagttaa ccgcggacgg aggcgaagcg ggagggccgg gatagtccca
      181 atcaaacgat acttggaagg gcttccacca tggatccacg ccggttgcag ggtcgacgtg
      241 gcggcccagt ccacccgcag atggaggatc gatcgcgtag gtcagtttga acgagccggc
      301 tctgggcatt tcgatggttg ccccgtagtg caacccgtca cgcgcgacca tgggcgtcaa
      361 cttcccctcg atggatttgg gggcctcgcc gtttgttttg ccgccctgcg gcgtgatcgt
      421 gtatcgaacc accaggtacg gaataaactc gtgctcggcg aagccgttgc gattaccctg
      481 cgtcgcatgg atatcggcct cgagatggat caacgccgac gatgcggaat cgaccattcc
      541 ttccatctgc acggggggga gccagacggc tttgatcacc atctggttta cctccacttc
      601 gtcaccgatc gggtactcgc ggacgcgcgc tattccggtc tggggctgag cctgctctgg
      661 cgtcggatcg acgggtgcaa gttcaatctt gctggatgcg gaaccgagtt gcttccggcc
      721 cttcaagtta aggttaaacg ccaggattgc gatcacgccg cccagaatca ggacgactac
      781 gagccgaccg gcccagcgct cccgcacgtc caaccacctt ttgccgggtc agccctgctt
      841 gggggccaga ccaattcccg aagccgccgg cccaccggca cgccaccacg acaagcgcgg
      901 aaccacaacc cacgccaaaa ccgcaccagc gagcagcatg ccctgcaccg acacgacttg
      961 aaggttggga tagatgcccg cccagggcaa ccctcgcccc atccaggcga ggttggtcgt
     1021 cagcaagatg ccggcattct gcaactcgaa cacgccgttg ccggcaaaaa cgatcgcaag
     1081 agcaaagagg aaaagcccgg aaaacttgaa gaacacgtgc atgggcaatc gcacgctggt
     1141 ctggcgaatc aaggctgcga ccactgcaag aagaatcagg ccgaggccca acccgatgat
     1201 cagcccgagg aaccccgatt gagtccgccc ttcgcttccc aggagtgcct ggtacatgag
     1261 cgaggtctcc gccccctcgc gaaacacggc cagaaacgca gtcagagcaa gcgttccctg
     1321 gcctcccagc tccagtccgt gtcgtgcctg agtcttgaga aaatccatcc accgcttggc
     1381 ctcgagctgc gagaccagcc agtaactgac gtaaaagagc acgcccgccg ccgccagcat
     1441 cacgacgcct tcaaggatct cgcgggcagc cccttgggca ttgataacca tgacgttcaa
     1501 cacgacggcg gtcacgaggc tggcaacggc ggccaatccc acgccccgcc aaattgcccg
     1561 ggaggcccgg accttggttt gggccgacgc tttgacctca tcgtgggtgg gcgccaccga
     1621 gccagtggtc gccctggcga ccaaggcgag cagcatggcc acaaccaaga tcacctcaac
     1681 gccctcacgc aagatcgtga tcagcgaggc cacgaacgcc gcgccaaatg ttccaaccgg
     1741 acgagcttcg agccgatcga ccaggttctc tacctcgttt gtcaagccgt tgacccgcga
     1801 cgacagtttg tctccgctca ggccgccggc cagatccccg cgcagcctat tgaactgcat
     1861 ttccagcggc cggaccgcct ggggattgcg ccccatcagg tagcgctcga gcggctcgaa
     1921 ctcggtcatg taaacagtcg tcagctcaga ggcagcatcg tccgattcac cgcgaaccgc
     1981 atattcctcg acgcggcgga gaccactctt gagcgcctgc agcagggccc tgggatcgcc
     2041 agcaaacccg gttgccggag ccagttcggt agggaaaact cccggacgag cttccgctcc
     2101 ggactgtccg ccaagagcat cgattcgcgt cgggtcggtt attcccttgg cgtcaagcgc
     2161 attcgagact tcgagcaagt catggagcag cttgtggcac gactgagcga gtgcctcacg
     2221 cggtatgcgt ttcactgcga catcacgcac ggccgttcgg atcgcatgga actgccgctc
     2281 cagctccccg gcacggacgt agcccaggta tttgcggact gcagtttcca tgtccgacga
     2341 ttcaaactca acccagtacg catcctcggc gagccgtcgc gccttggcca gctcattggg
     2401 ccgcgccgcc gcatcgcggc tcgcggccag cgcgacgctg atgcggtcga tcacatcagg
     2461 ccaacgccgt actcgcgctg ttttctgatc catacgacgc gcggtgggcc cgagagaact
     2521 agtgagcacg atggctggca caaccaagct attggcatcg ctcgtgacgg tgatttcagt
     2581 ttggaccgga tctcccatct cgtgccaggc cgtcaggaca tatcgcccct cggacacatc
     2641 atccaggcgg aaccgtccct cgcgaccgca aacctgtgcc caacgcgtcg ggctgacgat
     2701 cacatagccg cgcatgtgca tatggatgtc gcatgccagt ctcatgacgc caggttgagt
     2761 cggagtaaaa tcctggaact gtcccggtgc catcgcttga ttgaaggcaa agccgggcga
     2821 cacgatgtgg acgttgtgcg tttcgccgtc ctggttgcca aagcgcacgg tttgtcccag
     2881 cgcgatcgcc cgtacccggg gcataaattg cagctcacgt tgattgacca gtacgacctc
     2941 ggcggagggc aatttgcccg gctgcgatgt ggcacgttgg gcgagaccgt tttggtccga
     3001 gccggacgga gtcagataga cggccgccgg actgatcgcc ggtgagcaaa catcgggcat
     3061 ctggactacg cccgacacac tggtgccggc accgacttcc gccgccaggc cacaagacaa
     3121 cagccagagc aacgaacacg tctggaacag ccctatcact cgcgctcgac gcatcgaagc
     3181 cctcgagtaa ttaagatgcc accatgcatc ggcccacgat tgaaggtatg aagatttaca
     3241 attgagattg agtctcagtt acaagagaaa ttatagtgtt cggggtgacc gatcgtcaat
     3301 gcaatcgtta tcgtctattg cgagtggctg gtggttgtat ggattgccga gccaaatcag
     3361 aagagcgtgg aaatgccgcg ctggattatc cgaagacggc cgttcggaat ggacaccgtc
     3421 aagaaccggc agcgagctcg atcttatgga agccgaattt ccgcctgccc cgctgcggcc
     3481 ggtgtatcga agcgattatg gtgggcctgt cattgtcacg acgctcgagt ttggcggcaa
     3541 atcgatccgg ctcgcacgac cggcggaccc cgaccggctc ctcgacgacc cgcgggttat
     3601 cgactggaat cggagcgacg attacatgcc gtactgggcg tatctctggc ccagtgcgct
     3661 cctcttggcc gagtgggttg cacgcgagcc ttgggcggag agactggacg gggccatcgc
     3721 accggaggcg ctcgagatcg gctgtggact cgggctgcca ggcttggtcg cggtggctcg
     3781 agggctgcga gtccggttta ccgaccatga cccggcgcca ctcgaattcg tcgcgcgaag
     3841 tgcggcggag aatcgatttg attccggtca gtattcgatc cgccccctcg attggcgcag
     3901 cccgcctgac gagcggtttt cgatcatctt gggcgccgac gtgatctacg aactgcgtct
     3961 ggttcctctc gttgctgagt tgctgagccg tttgctcgcg cccgatggca tgggcgtgat
     4021 caccagcccc tatcgcgcgg ccgcgcaagg atttccggcc gcgctggcgg gtcttggcct
     4081 gacctgccaa gagaaatccg tgacggcacg gttgccggaa gggcaaacga tcgaaggaac
     4141 gatttaccac gtgagccggc cgaattcggc attcacgacg ggaatcttcc cgggtgcgtc
     4201 accctcgatt tgactgaagg gtcccgatga tcgagcggat gtcggcaact ccgatctcct
     4261 cgagctgaac ggtcaaatca tcgagaagcc gttcgcacgc ggtcggttcg taaaacgttg
     4321 cggtgccgac ctgaatggcc gaggctccag ccaccaagaa ttcgagggca tcgtcggccg
     4381 tggcgattcc cccgataccg ataatcggca gcgtgggaag cgcccgagcg acttcccaga
     4441 cgattcgcaa ggcgacgggc ttgatcgcgg tgccggagag tcca
//
LOCUS       DATAQL010001237         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5163773, whole genome shotgun sequence.
ACCESSION   DATAQL010001237 DATAQL010000000
VERSION     DATAQL010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5163773"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VHS97_21370"
     CDS             <1..193
                     /locus_tag="VHS97_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450818.1"
                     /translation="DGKKLASAGYGGNIYVWDVISAKPVFHQQVARFTMTYGLAWSPD
                     GKELAVAASDNKAYLLKLP"
     gene            complement(258..752)
                     /locus_tag="VHS97_21375"
     CDS             complement(258..752)
                     /locus_tag="VHS97_21375"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX3450819.1"
                     /translation="MKVKPPDSSGKPFDSDSTVGLPPYVIDTSVAVKWYIPEAHSVEA
                     KVFLGRRLDRHAPDYLPAEAASVVLKRVRSKDEKLRLTFDEGRMVLAALKTSPIQFHE
                     SGPLIDPAFALAAEIGASLYDGLFLALALQLGGQLVTADHKLFKKIEASRHADLVRWI
                     VPAQ"
     gene            complement(749..982)
                     /locus_tag="VHS97_21380"
     CDS             complement(749..982)
                     /locus_tag="VHS97_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450820.1"
                     /translation="MANVLVREIDERDLELLKRRALASGRSLQAELKRILELAARASD
                     EETARALAERVSAALAGRQHPDSADLIRKDRDR"
     gene            complement(1181..2491)
                     /locus_tag="VHS97_21385"
     CDS             complement(1181..2491)
                     /locus_tag="VHS97_21385"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450821.1"
                     /translation="MDLPVTRRHFLGTSAGSAVALRPGPLLAQAGALPSQKVTIGIMG
                     LNRGMQVIDALEKQPGVVIKYVCDVDSKRTDKAKSQLERNGKQRPQAITDFRRILDDR
                     DVDALFCEAPNHWHGPATILACSAGKHVYVEKPCSHNPWEGEQMIAAAKKHNRAVQMG
                     VQRRSTPGVMEAMTKLHQGVIGNVYCARSWYNNHRPSLGTGKQTSPPATLDYELWQGP
                     APRSPYHDNRIHYNWHWFWHWGNGELGNNGVHSLDLCRWGLNVEFPTTVVSSGGRYRY
                     HDDQQTPDTHVANYEFPGGKQITWQGLSCNPHPDSTGYVTFYGDKGALSLGEFGDYKI
                     YNADDRLIEKRPDDAQGRNPWLAVDTFHTAHFIAAIRNGDFFGLSAQIEKGHRSTLLC
                     HLGNIAYRTQRTLKCNPENGHVLDDAPAMAFWQREYESGWKPIV"
     gene            complement(2710..>3794)
                     /locus_tag="VHS97_21390"
     CDS             complement(2710..>3794)
                     /locus_tag="VHS97_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450822.1"
                     /translation="AQHGQSGRWVSELFPHVARHVDDLCVVNSMYTEGLAHGEATLRL
                     HTGSANFVRPSLGSWITYGLGTENNNLPSFVTIAPPRAHGGVQNYSSAFLPAVYQGTA
                     IGTADVPVKDASIPFLSNDKLPPALQRRQLDLIQQLNRSHLQQSEDDAQIEGMVQAYE
                     LGFRMQSVAPTVLDLAQESEATLKLYGVGTKETDDFGRQCLLARRFAEEGVRFIQIST
                     GYLWDQHEKLVSGHEKIARAVDRPISGLLTDLKARGLLEDTLVFWGAEFGRTPYTEGI
                     DGRDHNPEGYSVWLAGGGVKSGIAYGQTDEFGFSAVENKVHYHDLHATILYLLGLDHE
                     RLTYRYSGRDFRLTDVYGRVVQDILA"
BASE COUNT          718 a         1103 c         1143 g          830 t
ORIGIN      
        1 cgacggtaag aaactggcct cggccggtta cgggggtaac atatatgtat gggacgttat
       61 atccgccaag ccggtgtttc atcagcaggt cgctcgattc accatgacgt acggacttgc
      121 ctggagccca gacggtaaag agcttgccgt ggcggcttca gacaacaagg cgtatttgct
      181 caagctgccg tgagcggcgt ggcggaaagg tcggtgatat cgggccgggg agcgcctccg
      241 atcccgaatg tcctcggtca ctgagcgggt acgatccagc gaaccaggtc agcgtgtcgg
      301 ctggcctcga tcttcttgaa tagcttgtga tcggcagtga cgagctgccc gcctagctga
      361 agagccaggg ctagaaatag gccgtcgtac aaggaagctc cgatctctgc ggccaaggcg
      421 aaggctggat cgatgagcgg tcccgattca tggaattgga tcggcgaggt tttcaaagcc
      481 gccagcacca tgcgtccctc atcgaaggtc agccgcaatt tctcatcctt cgagcggacc
      541 cgcttgagaa cgacgctggc ggcctcggcc gggaggtagt cgggggcgtg acggtccaat
      601 cgcctgccca ggaagacttt cgcctcgacc gagtgggcct caggaatgta ccacttgacc
      661 gcgaccgacg tgtcgatgac gtaaggtgga agacccacgg tggagtctga atcaaatggt
      721 tttccagacg aatcgggcgg cttcactttc atcgatcgcg atccttgcgg atcagatcgg
      781 cactgtctgg atgttgtcgg ccggccaacg cggcactcac gcgctcggcc agtgcgcgag
      841 ccgtttcttc atcggaagct cgagctgcta gttccagtat gcgcttcagc tcagcctgga
      901 gtgaccgtcc ggacgccaac gcacgtcgct tgagcaattc caggtcccgt tcgtcgatct
      961 cgcgaaccaa gacgttggcc atcgcaatct cccgccgcgc cgtattgggt ccacttcagc
     1021 cgcttgcata atgatagcac gacgtcgccc gaatcgcaag atcgttttcc gatggacttg
     1081 cgatcgatcc tggtagtcag acgttccgtt caatccgtct ccgacgctcc gccatccacg
     1141 cagggaattt gtcacaacat ctcctgacgt ctgagttcgt tcaaacgatt ggcttccagc
     1201 ccgattcgta ttcgcgctgc cagaacgcca tggctggcgc gtcgtcgagg acgtggccat
     1261 tttctggatt gcacttgagc gttcgttgcg tccggtaggc gatattgccc agatggcaga
     1321 gcagtgtact ccggtgccct ttctcgattt gggcgctcag gccgaaaaag tcgccgttcc
     1381 gaatcgccgc gatgaaatgg gcggtatgaa aggtatcgac cgccagccac ggatttcgtc
     1441 cctgtgcgtc gtcgggacgt ttctcgatca gccgatcgtc ggcgttataa atcttgtagt
     1501 cgccgaactc gccgagcgag agcgctccct tgtctccgta aaacgtaacg taccccgtag
     1561 aatccggatg agggttgcaa ctgagtcctt gccaggtgat ctgcttgccg cctgggaatt
     1621 cgtagttggc gacgtgggta tcgggcgttt gctgatcgtc gtggtagcgg tagcgaccgc
     1681 cggaggagac gacggtagtg gggaattcca cattcaatcc ccagcgacag aggtccagag
     1741 aatggacccc attattgccg agctctccgt tcccccagtg ccagaaccag tgccagttgt
     1801 aatgaattcg gttgtcgtga tagggcgagc gcggggctgg tccctgccag agctcatagt
     1861 cgagcgtcgc cggcggggaa gtttgcttgc cggtccccag cgatggccgg tggttgttgt
     1921 accagctacg cgcgcagtag acgttgccga tgaccccttg gtgcagcttg gtcatggctt
     1981 ccatcacgcc cggcgtgctc cgccgctgca cacccatttg caccgcccgg ttgtgcttct
     2041 tggcggccgc gatcatttgc tctccttccc agggattgtg gctgcatggc ttttcgacat
     2101 agacatgctt gcctgcgctg cacgccagaa tcgttgcggg tccgtgccag tgattgggag
     2161 cttcgcagaa cagcgcgtcc acgtctcggt cgtccaaaat tctcctaaaa tccgtaatcg
     2221 cttgcggacg ctgcttgccg tttcgttcca actgcgactt cgccttgtcg gttcgtttgg
     2281 aatcgacgtc gcatacatac ttgatgacga cgccgggctg cttttccagt gcgtctatga
     2341 cctgcatgcc ccggttcaac cccatgattc cgattgtcac cttctggctg ggaagagcac
     2401 cggcctgtgc gagcaacggt ccgggcctga gagcgacggc ggatccggcc gacgtaccca
     2461 aaaaatgtcg gcgcgtaaca ggtaggtcca tggtgtacct ccggtggtaa agctcgttgt
     2521 aaagaaagag ttcgcgctga tgcgccgcga aataataagt tgagcgtatt tcggccccgt
     2581 agtggcacgg gcttccagcc cgtgtccgaa aataccacgg gcaagatgcc cgtgccactt
     2641 aagccagccg cgcctacttg cttaacttat actttcgcgg ctgctaagtt caccacgcgg
     2701 accgagcgat caggcgagga tgtcttggac cactcggccg tagacatcgg tcaggcggaa
     2761 atcgcgacct gaatagcgat aagtcaaacg ctcgtggtcg aggcctagca agtacagaat
     2821 cgtggcgtgc aggtcgtgat agtgcacctt gttttcaact gcggagaagc cgaactcgtc
     2881 ggtctgaccg taggcgatgc cactcttgac gccgccgccg gctagccaga cggaataccc
     2941 ctcagggtta tggtcgcgac cgtcaatccc ctcggtgtac ggggtacgtc cgaattcggc
     3001 cccccagaag accagcgtgt cttctagcag gccgcgggcc ttgaggtcgg tcagcagacc
     3061 ggagatgggc cgatcgaccg ctctggcgat cttctcgtgt ccacttacta gtttctcgtg
     3121 ttggtcccaa aggtaacctg tggatatttg gatgaaacgt acgccttcct cggcgaagcg
     3181 acgggccaac aggcattgcc ggccgaagtc gtcggtctcc ttggttccga cgccgtacaa
     3241 cttcagcgtt gcttccgact cttgggccag gtcgagcacc gtcggcgcca ccgactgcat
     3301 gcggaagccg agttcgtacg cctgaaccat cccttcaatt tgtgcatcgt cttcggactg
     3361 ttggaggtgc gaacggttca attgttggat gaggtcgagt tgccgccgct gcagcgcggg
     3421 gggaagctta tcgttagaga gaaaggggat cgaggcatcc ttcaccggga cgtcggccgt
     3481 gccgatcgcc gttccttgat agacggccgg cagaaacgcg ctggaatagt tttgcacgcc
     3541 gccgtgagcc cgcggcgggg caatcgtaac gaagctgggc aagttgttgt tttctgtccc
     3601 caatccgtac gtgatccacg atcccagcga cggtcggacg aaattggcgc tccccgtgtg
     3661 cagccgcagc gtggcttctc cgtgggcgag gccttcggtg tacatactgt tgaccacgca
     3721 cagatcgtcc acgtgccttg cgacgtgcgg aaatagttca gaaacccagc gaccactctg
     3781 gccatgctgg gcga
//
LOCUS       DATAQL010001238         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_736983, whole genome shotgun sequence.
ACCESSION   DATAQL010001238 DATAQL010000000
VERSION     DATAQL010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736983"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VHS97_21395"
     CDS             <1..427
                     /locus_tag="VHS97_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEX3450823.1"
                     /translation="YGGTSTSGVAGAGGLAQQYDANQNPAAVIAGLQACTIAIQNRQG
                     LSNCRGIFWEVGSLGMTMFNTIAPPNSSQYNWGACRFTGGGYPNDATFSNANSLHPGG
                     VNVLMADGSVRFIKNSINQNTWWSLGTRANGEVISSDSY"
     gene            521..1765
                     /locus_tag="VHS97_21400"
     CDS             521..1765
                     /locus_tag="VHS97_21400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX3450824.1"
                     /translation="MSVIQRHRPRRSKLIANKHRILSMIFREGSCSRLYLARKLNMNA
                     STIGNYVDDLLERGLLIEDHVGPTRRGRSPVPMWLNPDYGYFLGVDFEALRVRTVLTD
                     FAGKIVAQKEVGLKAGLGREAVLKTVVDAAKQTAEKAQSGRLFALGIAAPGQVDCRLG
                     RIVLYDLLPDFENVPLLDHFRPHFDCPVFVEENIRALTLAELLRGAGKGHRHFLCVAA
                     RSGIGMGIVINGQIYTGSHELAGKVGRTVLSPNDHPQTMTNLVSAKGIIRQALQLLKT
                     NRKTSLRRSLVDKGDDLSLADLVAAADAGDRRIIDLLEQVGKNLGLVAANLANLFAPE
                     KIILAGEVPSCCPLVRQTLELSFQQYTLNHILEHLVLADGALSGFAGAMGAAYLGFLK
                     TFPEQESVSLEVGESLVLGAGR"
     gene            2104..3342
                     /locus_tag="VHS97_21405"
     CDS             2104..3342
                     /locus_tag="VHS97_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450825.1"
                     /translation="MADETRAKNDRPRLGLIGAGGQGSGDARGAVHHGDFLAVCDVDR
                     RHAEKAKKDQRIGKDKADVYEDYRKILDRKDIDAVIIGTPDHWHTKICIDAMRAGKDI
                     YCEKPLTLTIDEGKKLSQVAKETNCVVQVGTQQRSDHNRVFLLAVAMVRAGRIGKIRK
                     VTAAIGGGPTGGPFPATNPPAELNWDMWLGQAPKVPYREHRCHGDFRWWYEYSGGKLT
                     DWGAHHVDIGQWAIGMENSGPNTIEVVKGVLPVEFKGGYPAVDDRYNTTTEFLVRAKF
                     ANDVVMDIRHDQENGVKFEGAKGKIFVTRDRIDLEGGAVDALYKNPVPESLLKELRKG
                     KPVDGHMANFFECIRDRSVPVSDVLSHHRALTTCHLANIALRLGRHKLTWDPAKEEIV
                     GDSEANAFQTRPQRTSYEIG"
BASE COUNT          721 a         1112 c         1084 g          674 t
ORIGIN      
        1 ctacgggggg acctcgacca gcggggtcgc cggtgccggc ggcctagctc agcaatacga
       61 tgcgaatcag aacccggccg cagttattgc cggactgcaa gcctgcacga tcgcgattca
      121 aaatcgccag ggcctgagca actgtcgcgg gatcttctgg gaggtcggct cgctggggat
      181 gacgatgttc aacacgatcg ccccgccgaa ttcgtcgcag tacaattggg gcgcctgccg
      241 gttcacggga gggggttatc ccaacgacgc gacgttctct aacgccaaca gcctccatcc
      301 gggcggagtc aacgtgctga tggccgacgg cagcgtcagg ttcatcaaga attcgatcaa
      361 ccaaaacacc tggtggagcc tgggcacacg ggccaacggt gaggtgatct ccagcgattc
      421 ttattgatca ggatccgaga ctttagcgct ctccgagcag cgagagcgct cgattgtcag
      481 taggtaccag gtgtatctcc aactctcgag aagactcctc atgagtgtga ttcaacgtca
      541 ccggccacgc agatcgaagc tgatcgccaa caagcaccga attctcagca tgatctttcg
      601 cgaggggagc tgttcgcgtc tttatctcgc tcgcaagctc aatatgaatg cgtccaccat
      661 tggaaattat gtggacgacc ttcttgagag aggcctattg atcgaggatc acgtcgggcc
      721 gacccggcgg ggccgctcac cggtgccaat gtggctgaac cccgactatg gctactttct
      781 gggagtggat ttcgaggctc tgcgcgtgcg gacggtattg accgacttcg ctggaaaaat
      841 tgtggctcag aaggaggtcg gtctcaaggc tggccttggc agagaggccg tattgaagac
      901 ggtcgtggac gcggcgaagc agacggcgga aaaagcgcaa agtgggcggc tgttcgctct
      961 cggcattgcc gcgcctgggc aggtagattg ccggctgggc cgcatcgtgc tctacgattt
     1021 gttgcccgac ttcgagaatg ttccccttct cgatcatttt cgaccgcact tcgattgtcc
     1081 ggtctttgtc gaggagaaca ttcgcgcgct caccctggcc gaactgctcc gcggggccgg
     1141 caaagggcat cgtcatttct tgtgtgtggc cgcccgctcg ggaatcggca tgggcattgt
     1201 cattaacggc cagatctata ccggcagcca tgagctggcc ggcaaggttg gccggacggt
     1261 tctctctccg aacgatcacc cacaaacaat gaccaacctt gtctccgcaa agggcatcat
     1321 ccggcaagca ctccagttac taaagacaaa ccgcaaaacg tcgctgcgca ggtcgcttgt
     1381 cgacaagggc gatgacctct cgctggccga cctggttgca gccgcggacg cgggtgatcg
     1441 ccggattatc gacctgctgg aacaagtcgg gaaaaacctg ggactggtcg cggccaacct
     1501 cgccaacctg ttcgcgccgg agaagatcat cttggctggt gaagttccct cctgttgccc
     1561 gttggtccgc cagacgctcg aactgtcgtt tcaacaatac acattgaacc atatcctcga
     1621 gcacctggtc ctcgccgatg gggcgttgtc gggctttgcc ggagcaatgg gagccgccta
     1681 tttggggttc ttgaagactt ttcctgagca ggaatccgtc tcgctggagg tcggagagtc
     1741 gctggttttg ggggccggca gatagccggt ccgtaatgcg cgcagaccat ttcggtctac
     1801 cggaatgtca atgattcgac cgaatgacgc tcgagacgaa ggtgaccgcc agatcgcctt
     1861 tccctacggg gcggacccga tcgtccctct tctccaccac cctcgccagg ggttatattc
     1921 ggcttctata gtcaagcccg aggccccccg gacggcgtag atccgaggag tccggccggg
     1981 tcataattct tgctcgaggg tccaccactg atgattatcc gcacgcacaa ccgcagggaa
     2041 ttccttcgcg tctcggcgat gagccccgtt ctcctcccct attttgcgcc tttcggccgc
     2101 accttggccg acgagacgcg ggccaagaac gaccggccgc ggctcggcct gattggcgcc
     2161 ggcggccagg gctcaggcga tgctcgcggc gccgtgcacc acggcgattt cctggccgtc
     2221 tgtgacgtcg accgccgcca cgccgaaaaa gccaagaaag accagcgcat cggcaaggac
     2281 aaggccgacg tctacgaaga ctaccgcaag atcctcgatc gcaaggacat cgacgccgtg
     2341 atcatcggca cgcccgacca ctggcacacc aagatctgca tcgacgcgat gcgcgcgggc
     2401 aaggacatct actgcgagaa accgctgacc ctgacgattg acgaggggaa aaagctcagc
     2461 caagtcgcga aggagaccaa ctgcgtggtg caggtcggca cccagcagcg gagcgaccac
     2521 aatcgcgtgt tcctgctggc cgtcgccatg gtccgcgccg gccggatcgg caagatcaga
     2581 aaggtcacgg cggcgatcgg cggcgggccg accggcggcc cgttcccagc gaccaatccc
     2641 ccggccgagc tgaactggga tatgtggctg ggccaggccc ccaaggtccc ctaccgcgag
     2701 catcgttgcc acggcgattt ccgctggtgg tacgaatatt ccggcggcaa gctcaccgac
     2761 tggggcgccc accacgtgga catcggtcag tgggccattg gcatggagaa ttccggacca
     2821 aatacgatcg aggtcgtcaa gggcgtgctt ccggtcgagt tcaagggagg ctacccagcc
     2881 gtcgacgacc gctacaacac caccaccgaa ttcctcgtcc gtgccaaatt cgccaacgac
     2941 gttgtcatgg atatccgcca cgaccaggag aacggcgtga agttcgaggg cgcgaagggc
     3001 aagatcttcg tcacccgcga ccggattgac ttggagggcg gcgcggtcga cgcactctac
     3061 aagaatccgg tgcccgagtc gcttctgaaa gagctgcgta agggcaagcc ggtggatggc
     3121 cacatggcga acttcttcga atgtatccgc gaccgttccg tgcccgtctc ggacgtcttg
     3181 tcgcaccacc gcgcgctgac cacctgccac ctggcgaata tcgcgctgcg gctgggccgt
     3241 cacaagctca cctgggatcc cgccaaggag gaaatcgtcg gcgactccga ggccaatgcc
     3301 ttccagacgc gtccccagcg gacctcgtac gagattggct gattgtcgga tcgcctccta
     3361 aaaaagcgtg gcccggattt ctgcgcgtgc gatcgagagt cgcccccgcg aataatcgac
     3421 cacggcccag ggccgtggtc gattaatttt gagacgcgac cccaagcctg tccgccccaa
     3481 ttcccggccg ccgggcgaac tgttcgacgg catcaagtct cgatgccgaa cccagggcga
     3541 tagcgcttgt ggatcagctc gtcagcctcc gtggcattcg aagccctcat g
//
LOCUS       DATAQL010001239         6173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2567631, whole genome shotgun sequence.
ACCESSION   DATAQL010001239 DATAQL010000000
VERSION     DATAQL010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567631"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VHS97_21410"
     CDS             complement(<1..372)
                     /locus_tag="VHS97_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX3450826.1"
                     /translation="MSDQADRLRQIVGARRGATSTLALTEDEPGATAAVSECASLRKS
                     RSLLFTSGKGGVGTSNVVLNLAIALAEIGQRVVVLDADIGLANLDLLCGLTPRHDLGD
                     VLAGRCRLVDALVSGPGDIQIV"
     gene            complement(474..1601)
                     /gene="flhF"
                     /locus_tag="VHS97_21415"
     CDS             complement(474..1601)
                     /gene="flhF"
                     /locus_tag="VHS97_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX3450827.1"
                     /translation="MQSKTYRAKTMKDALTRVRRDLGGDAVILSAREVRRRRLFGLGP
                     RALVEVVATDTMPSERDFTSRQGSAAVIGSPASANVSAQVGEQLGRLHAMVETLSRHG
                     HVEHLLPDLPRALVPTYSQLIEADVPEVLTHRLMRHVADTLDPDQIDRPDAIRAALCE
                     AVELSIPVAPPIKAVAGTRRVVALVGPTGVGKTTTVAKLAANFKLAHGARVGLVTVDT
                     YRIAAVEQLKTYAEIIDLPLSVVNDPSEMPRALDEQGAVDLVFIDTAGRSPRDEVKIR
                     ELAEFLLQAKPDEVHLVLSAVAGERSLRSAVDRFAMVQVDRLILTKLDEAESLGGILA
                     VLGLYSRPLSYLTTGQAVPDDIEPADRKRLARLILRHDDLS"
     gene            complement(1695..3950)
                     /locus_tag="VHS97_21420"
     CDS             complement(1695..3950)
                     /locus_tag="VHS97_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX3450828.1"
                     /translation="MPPTTSSANEAGTAGVLVRGLVMPITIVGAILVFIVPIPPMVLD
                     VLLSANLTVAVVVLLTTLAIGRPQEFSAFPVILLTTTLTRLVLNVATARLVLTRGGVD
                     GLRAAGDVIRAFGSFVAGDQVIVGVILFSILVVIQFVVITQGATRISEVAARFTLDGL
                     PGRQMAIDADLHAGLIDQYEAHRRRDEVYRQADFFGAMDGASRFVRGDAIAGVVILLV
                     NIGGGLVLGMVNHGMSLTQAVDVFTKLTIGDGLVSQIPAFLTSLAAGLIVTRSSSSSD
                     LSSDVVSQLFSKPSVLGTAAVFLGLMALTPLPKVPLLALGGAMGAAAFILSSRRVDNG
                     TDVAVGDTRDTGGFPSQTHARKGARPASLSSSRDRVSSLSSHEESEPTTVSADSSSER
                     MDDLLHVDPLELEIGFRLIGLADPTRGGDLLSRLKTVRQRVARQMGLIVPQVRIHDQI
                     GLPPNEYRVKIRGTIVGQGTAYAGRLLAVPPAGLVTRPDGRDGVEPLTGQPAVWIHAD
                     GREVAELAGCRVHESSAVVANHFGEIVANHADELLTREQVERLLDRVRSTAPTLAGEV
                     VPGLLRAGELQRILQNLLRERVSIRDLETILETLAVHAGKAKDIDLLTEQVRGSLARQ
                     ITETYRGSDGRIRVVILARPLDDRIAAAASQAETRPAEALGEDTIKSIVRAVAVAVGT
                     LIENDFPPVILTTPAARAVLKDLTRADLPRLVVLSQREIPRDTPIEVLGSVVEEEPEA
                     VTTITTTEAFA"
     gene            4498..6111
                     /locus_tag="VHS97_21425"
     CDS             4498..6111
                     /locus_tag="VHS97_21425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3450829.1"
                     /translation="MAGPRGRATWTRQFGPANGRPPRSRATRILVAGLLSIASAGCAG
                     IKAHDARLRTTVADIGERIEANRGADLSPEATAVLARLQLLKLVHEDPVGAARLLEDR
                     LQTQSEPDGSLALAELSYHIGVIFQSGSLCEAMVRYRDAAVLAALAVADPGSRRTDLA
                     VDLHNRSLARLIRLAETKKARQTGSPNWRQILEAEGLAVLSSTAYLEPARIGDLRVAA
                     DYRIEGMGHIYRASGLGVPLVAHRWTERDDSKDVQEQFFPREMRIAATAIVSPGGGLL
                     GGEWRRNPATLQLLDPFMIRSTAIGQRQLALATDTSTPLATQVSRAHLATLEWTGLLE
                     SDFERLGVDTGLYMLRPYEPGKIPVVFVHGLVSSPRAWVQAINELQNTPELDAHYQFW
                     LFMYPTGRTIPTSAARLRQALLHARDMLDPDGSDAAFNHTVMVGHSMGGVLCKMMAQD
                     SELQLWNATITVAPEHLKAPPDFRKSLDEVMIFRPVPFVSRLVFIATPHRGSPVADSG
                     LGQAVSNRCVETSRRTRASPKSRHSMERM"
BASE COUNT         1193 a         1966 c         1909 g         1105 t
ORIGIN      
        1 cacgatctga atatcgcccg ggccgctcac aagagcgtcg accaacctgc aacggcccgc
       61 tagcacgtcc cccaaatcgt ggcgcggcgt caaaccgcac aagagatcga ggtttgccag
      121 tccgatgtcg gcatccagca ccacgacccg ctgtccgatt tccgccagcg cgatcgccag
      181 gttaaggacc acattggagg tcccgacgcc tcccttgccg cttgtgaaga gtagggatcg
      241 actcttgcgc agcgacgcgc actccgagac ggccgcggtc gcgccgggct cgtcttcggt
      301 cagggctagg gtgcttgtcg ccccccgcct cgctcccacg atctgccgta gtcggtcagc
      361 ctgatcggac attcgcagtg cctcccaccg aacccggaaa aagagaggtc cgtggtcaat
      421 tgaccttgga ccagagtggc aaaaaatggt cacggggccc gctgccgata cgcctaagac
      481 agatcatcat gtctgagaat taaccgcgcc aaccgcttac gatccgcggg ctcgatgtcg
      541 tcggggaccg cttggccggt cgtcagataa ctcaacgggc ggctgtaaag ccccagaaca
      601 gccaggatgc cgcccaagct ttcggcctca tcgagcttgg tcaagatcaa gcggtcgact
      661 tgaaccatgg caaagcgatc gacggcggat cggaggctgc gctcgcccgc gacggcgctc
      721 aagacgaggt ggacctcgtc gggtttcgcc tggagcagga attcggccag ctcgcgaatc
      781 ttgacttcgt cgcgcgggct gcgacccgcc gtgtcaatga aaacgaggtc gaccgcgccc
      841 tgttcgtcga gcgcccgcgg catctccgac gggtcgttga cgaccgagag aggcaggtcg
      901 atgatctcgg cataagtctt gagctgttca accgccgcga tccggtaagt gtcgacggtt
      961 acgagcccga cacgcgcgcc atgagccaac ttgaagttgg cggcgagctt ggcgacggtc
     1021 gtcgtcttgc cgacgccggt gggtcccacc aaggcgacga cccgacgcgt gccggcgacg
     1081 gccttgatcg gcggggcaac cgggattgac agctcgaccg cctcgcacag ggccgcgcga
     1141 atcgcgtcgg gacgatcaat ctggtcggga tcgagcgtgt cggcgacgtg cctcatcaaa
     1201 cggtgggtca aaacctccgg aacgtcggcc tcgatcaatt gtgaataggt cggaacgagc
     1261 gctctgggga ggtcgggaag caggtgttcg acgtgtccgt ggcggctcag ggtctcgacc
     1321 atggcgtgca gccggccgag ttgctcgccg acttgcgccg aaacgttggc cgaagcgggt
     1381 gagccgataa cggcggccga tccttggcgg ctggtgaagt cgcgctccga cggcatcgtg
     1441 tcggtcgcga caacctcgac cagcgctcgc ggacccagcc cgaaaagccg gcgccgacgg
     1501 acctcgcgcg ccgacaggat cacggcgtcg ccgcccaaat cccgccgcac tcgcgtgagt
     1561 gcgtccttca tcgtcttggc tcgataggtt ttcgattgca tcaacgccgc ctcttctcat
     1621 cagcccattg tccgattcaa gatttctgcg attcttctgc ccgagccgag cgtccagtgt
     1681 tccttatcac tggttcaagc gaatgcctcg gtagtcgtga tagtggtcac ggcctcgggc
     1741 tcttcctcga ccacactgcc caaaacctcg atcggagtat cgcgtggaat ttcgcgctga
     1801 ctcaagacga ccagccgggg taagtcggcg cgcgtcaggt ccttgaggac ggcgcgcgct
     1861 gcgggcgtcg tcaagataac gggggggaag tcgttctcga tcagagttcc gaccgccacg
     1921 gcgacggccc ggacaatgct tttgatcgta tcctcaccca gggcctctgc aggacgtgtc
     1981 tctgcctgac tggccgcggc ggcaatccga tcgtcgagtg gccgagccag gatcacgaca
     2041 cgtatgcggc cgtcagatcc gcggtaagtc tcagtgatct gtcgcgccag gctaccacgc
     2101 acctgctcgg tcaagaggtc gatgtctttg gccttaccgg cgtgcactgc cagtgtctct
     2161 agaatggttt cgagatcccg gatgctgacg cgctctcgga gcaagttttg caggattcgc
     2221 tgcaactcac cggcacggag cagcccggga acaacctccc cggccaaggt cggggccgtg
     2281 gaacggaccc ggtcaagcag ccgttcaacc tgttcgcggg tcaagagttc gtcagcgtga
     2341 ttggcaacga tctcgccgaa gtggttggcg acgacggccg acgattcgtg gacccggcaa
     2401 ccggcaagtt cggccacttc acggccgtcg gcatgaatcc agacggcagg ctgtccggtg
     2461 agaggctcaa ctccatcccg gccgtcggga cgggtgacca gccccgccgg cggcacggcc
     2521 aaaagccggc cggcataggc ggtcccctgc cctacgatgg ttccgcggat cttgacccgg
     2581 tattcattgg gcggcaggcc gatctgatcg tgaatccgca cttggggcac gatcaggccc
     2641 atctgacgcg ccacgcgctg gcgcacagtc ttgagcctgg agagtaggtc acccccacgc
     2701 gtcgggtctg ccaggccgat caaccgaaag ccgatctcca gctcgagtgg gtcgacatga
     2761 agaaggtcgt ccattcgttc cgagctggag tcggccgaca cagtcgtcgg ctccgactcc
     2821 tcatgcgacg ataaggaaga tacccggtct cgtgacgacg aaagagacgc gggtcgcgcg
     2881 ccctttctgg cgtgagtttg tgaagggaac cctccggtat ctcgagtatc gccgacggcc
     2941 acgtcggttc cgttgtcgac gcgccgggaa ctcaagatga atgcggccgc acccatggct
     3001 cctcccagag ccaagagcgg aaccttggga agcggtgtca aagccatcag gcccaggaag
     3061 actgcggccg ttcctaacac gctgggcttc gagaacagtt ggctgacaac atcgctgctt
     3121 agatcggacg atgatgacga gcgagtcacg atcagtcccg ccgccaagga tgtcaggaac
     3181 gccggaatct ggctgaccag cccatccccg attgtcagct tggtaaagac gtcgacagcc
     3241 tgggtcaggc tcatgccgtg attaaccatg cccaggacca gtccgccgcc aatattcact
     3301 agcaagataa cgacacccgc gatcgcatcg ccccggacaa accggctcgc tccgtccatg
     3361 gccccgaaga aatccgcctg gcggtagact tcatcgcggc ggcggtgcgc ctcgtactgg
     3421 tcgatcagcc cggcgtgcaa gtcggcatca atggccatct gtcgcccggg gagcccgtcg
     3481 agcgtgaacc gggcggccac ttcgctgata cgggttgcac cctgagtgat cacgacgaac
     3541 tgaatcacga ccaggatcga aaacaagatc acccccacga tcacctgatc gccggccacg
     3601 aatgagccga acgctcgaat cacgtcgccg gctgccctga gaccgtcgac tcccccgcgt
     3661 gtgagtacca accgcgccgt cgcgacgttc aagacgagcc gagtcagcgt ggtggtcaaa
     3721 aggatgaccg ggaatgcgct gaactcctga ggccgaccaa tagccaacgt cgtcaaaaga
     3781 acaacgacgg ccacggtcaa gttcgccgaa agcagaacgt cgagcaccat gggcggaatc
     3841 ggcacgatga acacgaggat cgcgccgacg atcgtgatcg gcatgaccag cccacggacc
     3901 aaaactccgg ctgtgccggc ttcgtttgcg ctcgaggttg tgggcggcat ccgttcccct
     3961 ccacaatccc tgttaatgcg gaacgggcgc tctctctgcc cgcgtgccca attttcacac
     4021 gggcgcgtcg cccgtgccac aagcgcaacc gcttgcgatc cctgtttgga tccggaatgg
     4081 aagaccgtga tttttcttat ttagaccgaa gtgtgaatcc ttgggacgtg tggggggacg
     4141 ataccgctga acgcggcagg tgtccagagc gaatcacgga tctcgactgt gcgggcaatg
     4201 tacaaaagtt tcgccctgtg ctggcaaggc caccggcagg aattaccgta cccggtctcg
     4261 tgggtgggca ccgcccaccg aactgggccc tggccggtgg gcagtgccca ccctacctgg
     4321 gtaagcttcg actcgccggt cgccgaaaga cgtattcgag ctcggctttt caagagtgga
     4381 tcggcctggt agtctgccgg cggctcgggc ccggcagaga atcgtgattg ctttgcgcat
     4441 ggatggtttc agcgaggctt gtactgggca ctaatgccga gctcacaagg atgaaacatg
     4501 gcgggccctc gtgggcgagc aacatggacg cggcaattcg gaccggcgaa cggacgaccg
     4561 ccgaggtcgc gggcgacgcg catcctggtc gcgggattac tcagcattgc gtccgctgga
     4621 tgtgccggca tcaaggctca cgatgcccgg ctccggacta ccgtcgcgga tatcggagag
     4681 cgcatcgagg ccaatcgcgg tgcggacttg agtccagagg cgactgctgt cctcgcgcgc
     4741 ctgcagttgc tgaagcttgt ccacgaagac cccgttggcg cggcgcggct gctcgaggat
     4801 cgtctccaaa ctcagtcaga gcctgatggg tctctggctc tggccgaact gtcttaccac
     4861 atcggcgtca ttttccagag cggatcccta tgcgaggcga tggtccgata tcgtgatgca
     4921 gccgttctcg ccgcactcgc cgtcgccgac ccggggagcc gtcggaccga tctggccgtc
     4981 gacctccaca atcgctctct tgcgcgattg atccggctcg ccgaaaccaa gaaggcccgg
     5041 cagaccggca gccccaactg gcggcagatt ctggaagcgg aaggactcgc cgttctcagc
     5101 tccaccgcat acctcgagcc cgcacggatc ggcgacctta gagtggcagc cgattatcgg
     5161 atcgagggca tgggccacat ttatcgcgcc agcggactgg gcgtgccgct ggtcgcgcac
     5221 cgatggaccg agcgcgacga ctcaaaggat gtccaggagc agttcttccc gcgggaaatg
     5281 cgaatcgcgg ccactgccat cgtatcgcct ggaggaggcc tcttgggcgg cgaatggcgg
     5341 cgaaaccccg cgaccttgca actgctcgac ccatttatga ttcggtcgac ggcaatcggc
     5401 cagagacaac tcgctctcgc aacagatacg agcacaccgc tggcgacgca ggtatcgcga
     5461 gcgcatctcg ccacactcga gtggactggc ctcttggaat cggacttcga gcgactgggg
     5521 gtagacacgg gcctttacat gcttcggcca tatgagccgg gcaagatccc ggtggtcttt
     5581 gttcatggcc tggtctcgag cccccgtgcc tgggtgcagg cgatcaacga attgcaaaac
     5641 acgcccgagc tcgacgccca ttatcagttc tggcttttca tgtacccgac cggccggacc
     5701 atccctactt ccgccgcgcg cttgcgccag gcgctcttgc acgcgagaga catgctcgat
     5761 cctgacggtt ccgacgcggc tttcaaccac acggtgatgg tcggccacag catgggcggt
     5821 gtcctttgca aaatgatggc gcaagactcg gagctccagc tctggaacgc cacgatcacg
     5881 gtcgcacccg aacatttgaa ggctcctcct gatttcagga aaagtctcga cgaagtcatg
     5941 atctttcgcc cggtcccctt tgtgagccgg ttagttttca tcgcaacccc tcatcgaggc
     6001 agcccggttg ccgatagcgg actagggcag gccgtgtcga accggtgcgt cgaaacgtcg
     6061 agacggacgc gcgcatcgcc gaagtcgagg cactcaatgg agcggatgtg atctccccgg
     6121 aactgcgcgg gcacgccatc aatgccatca gtaacctgcg caccgacagc ccg
//
LOCUS       DATAQL010001240         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5164227, whole genome shotgun sequence.
ACCESSION   DATAQL010001240 DATAQL010000000
VERSION     DATAQL010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5164227"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VHS97_21430"
     CDS             complement(<1..1264)
                     /locus_tag="VHS97_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450830.1"
                     /translation="MRYSAVMLLVLVGTARAAERPMVFNHPAISAKHVVFGYAGDFWV
                     VDKEGGEARRLTSGIGLETHPVFSPDGTQVAFGGEYDGNLDVYLVPIAGGEPKRLSYH
                     PDPDLPVAWTPDGKSVLFRSTRSSYGRFTQLFTISAEGGPETAVDLPMAEEGSYSPDG
                     GKLAYVPFTNTRPFPGRYMAWKRYRGGSAPFIWIADLKSLDIDKIPRTDSNDFNPMWV
                     NSTVYFLSDRDGLTTLFAYDLKTKKVERVLEPTDSDIKSASACADAIAFARIGGVFVY
                     DLKDNKARKLDIEVRGDLPGVRPRLKKVGERIRNIGLSPAGARVVFESRGEILTAPAE
                     KGDARNLTMSAGIADRDPAWSPDGKSIAYFSDESGEYELHLKPQDGLGDVKKYKLGDA
                     PSFYYSPRWSPDGKRIAYSDKRKQLWYID"
     gene            1579..2148
                     /locus_tag="VHS97_21435"
     CDS             1579..2148
                     /locus_tag="VHS97_21435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3450831.1"
                     /translation="MARPLSEDKRTAILAAATELVAMLGVSAPTAKIAKGAGVAEGTL
                     FTYFANKDELLNRLYQELKTDLRDAIMAGYPADKSLIDRSRHFWDRYIAWGSAHPLKQ
                     RAIRQLAVSDRITEECKKLVGDEFGQVNDIMRECAAGGAMRRQPPSFVSAILSAIADT
                     TMDFIAREPAHAKRYTKAGFDAFWKAVAD"
     gene            2223..3083
                     /locus_tag="VHS97_21440"
     CDS             2223..3083
                     /locus_tag="VHS97_21440"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3450832.1"
                     /translation="MSKVWLVTGSASGLGRNIAEAVLASGERLVATARDLDRLQDLVE
                     KYGDQVRTAPLDVANEEAAYAAVQVAMDAFGRLDVVVNNAGYGDIAPFEQLTSERFKA
                     LIDTNFYGVVNMTRAALPIMRKQKSGCILQISSVGGRLARPGNTGYHAAKWAVGGFTE
                     SLAQEVAPFGVRVCALEPGGMRTNWGVRANKDTPDLLADYEPSVGAGAKALKSYWGHE
                     TGDPVKVAQLVLRLATSDSLPAHLLLGSDAVQFAGQAEATRAADAARWREVSISTDVD
                     ARGPLPAIRF"
     gene            3105..>3438
                     /locus_tag="VHS97_21445"
     CDS             3105..>3438
                     /locus_tag="VHS97_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450833.1"
                     /translation="MATPLSTIAKSVLASQAMRNKGGGEDDLALVPHPKVQVRESGDG
                     EVPDRPAIRRRLPRLEDQRRLGVGPCRSDKGVERTVQNIGVGAHVPVEGPMEQSQLPA
                     PLFVILGGG"
BASE COUNT          717 a          988 c         1040 g          693 t
ORIGIN      
        1 ggtcgatata ccagagttgc tttcgcttgt cactgtaggc gatccttttg ccgtcgggcg
       61 accaacgagg actgtaatag aaagacgggg catcgccgag cttgtacttt tttacatcgc
      121 ccagaccgtc ttgcggcttc aggtgaagct cgtactcccc cgattcatcg gaaaagtagg
      181 caatcgattt gccatcgggc gaccacgcgg ggtcgcggtc ggcgatgccg gcgctcatgg
      241 tcaagtttcg cgcatcgcct ttctcggcgg gcgcggtcag gatctcgcca cgggactcga
      301 atacgacgcg ggcaccggct ggcgacagac cgatgtttcg gattcgctcg ccgacctttt
      361 tcaaacgagg ccggacaccg ggcaggtcgc cgcgaacctc gatatcgagc ttcctcgctt
      421 tgttgtcctt caagtcataa acaaacacgc cgccgattcg ggcaaacgcg atcgcgtcgg
      481 cacaggccga agccgatttg atgtctgagt cggtcggctc caacacccgt tccactttct
      541 tcgttttcag gtcgtacgca aacaacgtgg taagaccatc gcgatccgac aggaaataaa
      601 ccgtggaatt gacccacatc ggattgaaat cgttggagtc cgttcgcggt attttgtcga
      661 tgtcgaggct tttcagatcc gcgatccaga taaagggcgc gctgccaccg cggtaacgct
      721 tccacgccat gtaccggccg ggaaagggac gcgtattggt aaaagggacg taggcgagct
      781 ttccgccgtc gggggaatag ctgccttctt ctgccatggg caagtcgacc gcggtttctg
      841 gcccaccctc cgccgatatc gtaaacagct gggtgaatcg gccatagctc gaacgcgtcg
      901 agcgaaacag gaccgatttg ccatcaggcg tccaggccac aggcaggtcg gggtcgggat
      961 gatacgacaa tcgcttcggc tctccgccgg cgatcgggac gagataaacg tcgaggttgc
     1021 cgtcgtactc gccaccgaat gctacctgtg ttccgtccgg agagaagacg ggatgcgttt
     1081 ccaggccaat gccggaagtc aagcgccgtg cctctccgcc ctctttgtcg acgacccaaa
     1141 agtcccccgc atagccaaac acgacgtgct tggcgctgat ggcaggatga ttaaaaacca
     1201 tcggccgttc ggctgcccga gccgttccga ccaacacgag caacataacg gcagaatagc
     1261 gcataggccc tccttcaagc ggggttttag cggcgtgctc tttaagaaca gagtggcgat
     1321 attacgcaat tcgtcgaaca ttccctaata tttccaataa cctcgaagaa aaaaggcacg
     1381 cactgcaacg caaagtcgca ccgcaatgcc agtccgcctc gccgaggtga gctccatcga
     1441 tcgagatggt aactactttt gcgccttcgc ggctttgctt gagaattgaa taccccgcgc
     1501 ccaatcgccc cgcggattct gcttgaccac tcggcacgtt tttcctatga ttgagcaggc
     1561 actcattttt ggagagaaat ggcacgccca ctcagcgaag acaagcggac cgcaatcctc
     1621 gcggcggcta ccgaattggt ggccatgctc ggcgtgagcg caccgaccgc gaagattgcc
     1681 aagggtgcag gcgtggcgga aggcacgcta tttacgtact tcgccaacaa ggacgagctg
     1741 cttaatcggt tatatcagga actcaagacg gaccttcgcg acgccatcat ggccggctac
     1801 cctgccgaca agagcttgat cgaccgcagt cgccacttct gggatcgcta catcgcttgg
     1861 ggttcggcac atcccttgaa gcagagagcg atcaggcaac tggcggtgtc cgaccgcatt
     1921 accgaagagt gcaagaagct cgtcggggac gaattcggac aggtcaacga catcatgcgt
     1981 gagtgcgcgg ccggcggtgc aatgagacgc cagcctccgt cgttcgtgtc ggcgattttg
     2041 agtgcgatcg ccgatacgac aatggatttc atcgcccgcg agcctgcgca tgcaaagcgc
     2101 tacacaaagg ctgggtttga cgcgttctgg aaggcagtcg ccgactgact ttttttgttt
     2161 cgtgaatgag cgagcgctcg ctcatgtgtt actctgtgtt tgaaagtgaa tgggaggttg
     2221 tcatgtctaa agtctggctg gtgaccggca gcgcgagcgg tttgggccgc aacatcgccg
     2281 aagcagtgct cgcatcgggc gagcgcctcg ttgcgacggc ccgcgatcta gatcgccttc
     2341 aagatctggt cgagaagtac ggcgatcagg ttcgcaccgc tcccctcgat gtggcaaatg
     2401 aggaggcggc atacgcagca gtgcaggtgg cgatggacgc gttcgggcgt ctcgatgtgg
     2461 tcgtgaacaa cgcgggctac ggcgatattg cgccgtttga gcagttgact tcagagcgat
     2521 tcaaggccct gatcgatacc aacttctatg gcgttgtgaa tatgactcgc gctgcgctac
     2581 cgataatgcg aaagcaaaag agcggctgca tccttcagat atcgtcagta ggcggtcgtc
     2641 tggcccgtcc cggcaacacc ggttaccacg ctgccaagtg ggcggttggg ggattcaccg
     2701 agtctctcgc ccaggaagtg gccccttttg gcgtcagggt atgcgcgctc gaacccggtg
     2761 gaatgcggac gaactgggga gtacgcgcga acaaggatac acctgacctg ctcgcggatt
     2821 acgaaccatc cgtgggagcg ggagccaagg cgctgaaatc ttactggggc catgagaccg
     2881 gcgatccggt caaggtggct cagctcgtac ttcgcctggc taccagcgat agtctgcccg
     2941 cacatctcct gctgggcagc gacgccgtcc aattcgctgg ccaagccgag gcaacacgag
     3001 cagccgacgc agcacgctgg cgagaggtta gcatctcgac agacgtcgac gcacggggac
     3061 ctttgcctgc aatccgattc tgagcgggcg gaaggatttt tcgattggcg acgccgctga
     3121 gtacgatcgc gaagtccgtc ttggcgtccc aggccatgag gaataaaggg ggcggtgaag
     3181 atgatcttgc ccttgtgccg cacccaaagg ttcaggtccg tgaatcgggc gacggcgagg
     3241 tgccagatcg gcccgcgatc cggcgtcggc ttccgcgcct cgaggatcaa aggcgcctcg
     3301 gggtcggtcc ctgccgaagt gacaaaggag tagagcgcac cgtccagaat attggggtcg
     3361 gtgctcacgt accggtagag gggccgatgg agcaatcgca gctcccagct cctcttttcg
     3421 tcatcctcgg tggaggct
//
LOCUS       DATAQL010001241         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5317598, whole genome shotgun sequence.
ACCESSION   DATAQL010001241 DATAQL010000000
VERSION     DATAQL010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5317598"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VHS97_21450"
     CDS             <1..426
                     /locus_tag="VHS97_21450"
                     /inference="COORDINATES: protein motif:HMM:NF015180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HEX3450834.1"
                     /translation="SLLCAVHCAVTPMAMALLPLVGLEFLGAEATECTLVGLSIVVGS
                     VSLVLGYGRHRSVRTLGIFSCGLILLVAGRVTETWGVEGVGVVSAVAGGCVVATAHIV
                     NRRLCRNCALCRQREEAVARSTGRFQAEGLDSEAQAQWG"
     gene            506..1873
                     /locus_tag="VHS97_21455"
     CDS             506..1873
                     /locus_tag="VHS97_21455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450835.1"
                     /translation="MAKTARSALLLFTGVALGVAGIVITGPRRLQNLSLSWVADRARS
                     VPEPTREEAGVPANAEPPATEWVDDGWCAADKQANGSEPAAVSEQDQTCRRLLPVVRL
                     STALTARRIGLEFGTALRRRHAHRLAANAEIDYAAHDYAEVRPRVMGLIREVRADEGE
                     KVRKGDVLVVIDSAEVGTAKAQYLTATAAAELANVNRTSAKVLVDERIVSAKKGIESQ
                     SALNQAKAELLNAKQRLRNLGLTDQDLTEISRREDTSSMLNVVAPIDGTLVERLAVIG
                     VAVERTTLLFVDADISRMWAWIDVYETDIAQVAPGQAVTFTISGTDSPVFTGRVELIG
                     AAVNPATRTIRVRAELANTDGKLRAKQFGQAEIQVGAEHETVVVPKEAVQSDGQNDLV
                     FLSRPDGAFRPQRVKTRLMESPAEIEIEWGLVPGQRLVTTGAFLLKSEMFKAIFGDEI
                     LGGEG"
     gene            1960..>2205
                     /locus_tag="VHS97_21460"
     CDS             1960..>2205
                     /locus_tag="VHS97_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3450836.1"
                     /translation="MLNALIGFSLKNRFAVMLLAALLVYVGIESALHLPLDAFPDTTP
                     NQVQINSVAPALAPEEIERQVTLPVELSLGGMKGLQEV"
BASE COUNT          396 a          694 c          720 g          395 t
ORIGIN      
        1 tcgttgctct gcgccgtgca ttgtgccgtg acgcccatgg ccatggctct tttaccgttg
       61 gtcggtcttg agttcctcgg ggccgaggcc acggagtgta cgctggtcgg gctctccatc
      121 gtcgtaggaa gtgtgagcct agtcctgggc tacgggcggc accgctcggt tcgcacgttg
      181 ggcatcttct cgtgtgggct gattttgttg gtcgctggcc gagtcaccga aacatggggc
      241 gtcgaaggcg tcggcgtcgt ctcggcggtg gccggcggat gcgtcgtcgc caccgcccac
      301 atcgtcaacc gccgcctttg tcggaactgc gccttgtgcc ggcagcgaga ggaggcagtc
      361 gcccgctcga ctgggcgatt tcaggccgag gggctggaca gcgaagcaca ggcgcagtgg
      421 ggctaggtcg agatcgttca gcgacaatcc cgcccttggc tgccttcgaa agatagcgat
      481 caccttcgac tgatggacca tcgccatggc caagaccgcc cgctccgcct tactactctt
      541 caccggtgtc gccctggggg tcgctggaat tgttatcaca ggaccccgtc gcttgcaaaa
      601 tctgtccctg tcatgggtag ccgatcgggc gaggtccgtc ccagaaccga cccgcgagga
      661 ggccggcgtc ccagcgaacg cggaaccccc ggcgacggag tgggtcgacg acggctggtg
      721 tgcggcagac aagcaggcga atgggtccga gcccgccgcc gtgagtgagc aggatcaaac
      781 ttgccggcgc ctgcttccgg tcgtccggct atccacggcc cttacggccc ggcggatcgg
      841 cctggagttc ggaacggcct tgcgccgccg gcacgcgcac cggctcgcgg ccaatgccga
      901 aatcgactac gccgcgcacg attacgccga ggttcgcccc cgcgtgatgg gcctgatccg
      961 tgaagtccgc gccgacgagg gggagaaggt ccgcaaagga gacgtcctcg tcgtcatcga
     1021 ttcggctgag gtcggcacgg ccaaggccca gtacctcacg gcgacggccg ccgccgagct
     1081 ggcgaacgtc aacagaacga gcgccaaggt cctggtggat gagaggattg tttcggcgaa
     1141 aaaggggatc gaatcacagt ccgccttgaa ccaggcaaag gccgagctgc tcaatgccaa
     1201 gcaacggttg cgcaacctcg gcttgacgga ccaagacttg acggagattt ctcggagaga
     1261 agacacctcc agcatgctga acgtcgtcgc accgattgac ggcacgctgg tcgagcggtt
     1321 ggcagtcata ggtgtggcgg tcgagcggac cacgcttctt tttgttgacg ccgacatttc
     1381 ccggatgtgg gcatggatcg acgtctacga gaccgacatc gcgcaagtcg ctcccggtca
     1441 ggcggtgacg ttcacgatct ccgggaccga cagcccggtc ttcactggcc gagtcgagct
     1501 gataggggcc gcggtgaacc ccgcgacccg cactattcgc gtccgcgccg agttggccaa
     1561 cacagacggc aaactgcggg ccaaacagtt cggccaggcc gagatccagg tcggcgcaga
     1621 gcacgagacg gtggttgttc ccaaggaggc ggtccagagc gacggccaaa acgacctcgt
     1681 gttcctctct cggcccgacg gcgccttccg ccctcagcgc gtcaagaccc ggttgatgga
     1741 gtcgcccgcc gagatcgaaa tcgagtgggg cctcgtgccc ggacagcggt tggtcacgac
     1801 cggggcgttc ctgctcaagt cggagatgtt caaggcaatc tttggcgacg agatactcgg
     1861 aggcgaaggt tgagccgtcc gcgcccgcgt gacagtcgtc acggacctgc tgtgacgacg
     1921 ccgcctcaac cgtaaataac ccgaaaggcg atctccacga tgctcaacgc cctgatcggg
     1981 ttcagcctca agaaccgatt cgccgtgatg ctgcttgcgg ccttgctcgt ctacgtcggc
     2041 atcgagtcgg ctctgcactt gccgctcgat gcttttcccg acacgacccc caaccaggtc
     2101 cagatcaatt cggtcgcccc ggcccttgcg ccagaggaga tcgagcgtca ggtaaccctc
     2161 ccggtggaat tgtccttggg ggggatgaaa ggattacaag aggta
//
LOCUS       DATAQL010001242         5811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_430857, whole genome shotgun sequence.
ACCESSION   DATAQL010001242 DATAQL010000000
VERSION     DATAQL010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430857"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1736)
                     /locus_tag="VHS97_21465"
     CDS             complement(213..1736)
                     /locus_tag="VHS97_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450837.1"
                     /translation="MNRGLRRRRFLQMASALGLGAELAPWASLRTITPLKGEEAQLKP
                     EMVKFRPEIEPVVRLIEDTPRDRALEMAITQLKKGLSYNQLLSGLFLAGIRNIKPRPV
                     GFKFHAVMVINSAHVLGQSAAVTERLLPLMWALDNFKSSQAQDVKEGDWVLAEVDECR
                     LPKTHRAKEEFVRAMEVWDPEAADAAIAALCRSSGAAETMEPLWRVAVRDQRNIGHKA
                     IFAAQSWRTLQTIGWVHAEPVLRSLVFGMLDLQGDSRPRPVGPYEANLAIAPKIREEW
                     TIGRDDPAATQALLQVIRQATPEAASAEAVKLLNQGVSPGSLWDAAILAACELLMHSP
                     GILALHATTATNALHYIYGASGDDTTRRLALLQAVGWQPMFRGRAKLTDATGIDSLKA
                     SPDEPLSGGDEAVGEVFATISEDRKKAGAKAMAYLARGGSPDLIFDAARRMIFHKGRD
                     SHDYKYGAAVWEECLWSTEPKWRNQMVAASMSNFPGAKTADSPLMIRARDALATVMS"
     gene            complement(2042..3439)
                     /locus_tag="VHS97_21470"
     CDS             complement(2042..3439)
                     /locus_tag="VHS97_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3450838.1"
                     /translation="MEKSRQGGLRILFADDEAHLRDLMQMELPRLGHEVTVCADGAAA
                     IRALERSLYDAALLDMRMPGFTGIEVLGQIKQISPETQVIILTGHATVDTAVQALRLG
                     AFDYLTKPCKWAELEMILNRITERRDMANKTAALETRLKAAEGSPLLIGETPTMQQVR
                     RLIDTIAPTDASVMILGETGTGKELVARSLHEKSRRTDRAFIPVNCGALPENLVESEL
                     FGHRKGAFTGADTNRKGLFEVANGGTLFLDEVGELDKSVQVKLLRFLESGEIRRVGEN
                     EPFRVDVRVLCATNRDLRDMVANELFREDLFFRLNTFEIFLPPLRERRTDIAALAKHL
                     LTRHASRRGLTESTIVPESIELLMGHEWPGNIRELANMIERATILAGNSPILPEHLPT
                     QLPIRSKSHQSAPTSIGPPHFQVPEGSPTLRDVEMKYIQTILEKHQGNKPAASRELGI
                     SLKTLYNKINQLQHS"
     gene            complement(3515..5686)
                     /locus_tag="VHS97_21475"
     CDS             complement(3515..5686)
                     /locus_tag="VHS97_21475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450839.1"
                     /translation="MNIASFASAPSGATARGVDSAHQVLVVQVRQKGRQGSLGPGPRI
                     AVPKLLDKSSWLSQNVLTASDAPSARLETYGRAGGRGKRPAPEIRAKFGAALRPRRGA
                     RPEVYAPAVQAVPLPTLPATRAYNAESVGVHGERGTSICAFQPDREFCKIRHRSRIDA
                     ASRGTPSLQTRRHDKDVVMIRWPIRLKLIVGLSVVVGMMLILMGASIFGLHSFHMSYL
                     TLVDQLRELGASAELIESVFRLHAPREGTDAEMEELHEQVVVAQDALLVYFRQLKKNT
                     NQGSRVDNGTDELGLAFLIDDDLAAIRTELDPSGTTAAFLLPGTSFYVRDHPELLPPV
                     KPATGIKSRIERLHDTVMQLPKKLFSDFWQVLRLSQLKYQTSRVIVWVSALMVLAMLC
                     GLMFLFQRWVLFPLRFLQRGVRHVAHGSFDYKINLASGDEMQDLAEAFNDMTAKISMT
                     YADLERQVQERSRQLVRSERLAGVGFLAAGVAHEINNPLASIAFCAEALDHRLERLLR
                     TSADPDHRVVSNYLKMIQEESFRCKNITEKLLDFSRCNDIKRERIDLASLIQGVVDMI
                     RHIGKYTGKTITFHPREAVMAHVDAQEIKQVVLNLIVNALESMSSNGTIRIEARYNQG
                     MAEMVFTDSGCGMSPDVLENIFEPFFTKRRDGKGTGLGLSITHRIVNQHHGEIMAFSA
                     GENLGSTFTVRLPIHPTEIGEDGLARSSQLRRRSESAAAAL"
BASE COUNT         1097 a         1730 c         1786 g         1198 t
ORIGIN      
        1 cctacgttct catgtattag ctgatctgcc ggtgactcta acgcgagaaa acctttatca
       61 gatttggtcg taattccgtg gtggggcgaa cctgaaggta gccgaaggtg caggagcaga
      121 agtcgtgcgc gagcgttgcg cgccgcggac gacgttcacc tgcaccctct gtgattgaca
      181 gagacgatgg ctgaaaccgg ttcctgccag gctcagctca tcacggtggc aagcgcgtct
      241 cgcgctcgga tcataagggg gctgtcagcc gtcttggcac ccgggaaatt ggacatggag
      301 gcagcgacca tctggttgcg ccatttaggt tccgtcgacc agagacattc ctcccaaacg
      361 gcggcgccgt atttgtagtc atggctgtcg cggcctttgt ggaagatcat ccgccgagcg
      421 gcgtcgaaga tgagatccgg ggaaccaccg cgggccagat aagccatcgc cttggcgcca
      481 gctttcttgc gatcttcgct gatcgtcgca aacacttcac caacagcctc gtctccaccc
      541 gatagcggct cgtccgggct ggctttgagc gagtcgatgc cggttgcgtc ggttagcttg
      601 gcacgtccgc gaaacatagg ctgccaaccg actgcctgga gcagagcgag ccgacgggtc
      661 gtatcgtcac cgctcgctcc atagatgtaa tggagagcgt tcgtagccgt cgttgcgtgc
      721 agtgccagga ttccggggct gtgcatcaag agctcgcacg cggccagaat ggcggcatcc
      781 cagagagaac cgggcgaaac tccctgattc agaagtttga cggcctcggc cgaggcagct
      841 tcgggtgtgg cctgtcggat aacctggagc aacgcctgcg ttgctgcggg atcgtcgcgg
      901 ccgatcgtcc attcctcgcg aatcttgggg gcgatcgcca gattcgcctc gtacggaccg
      961 accggtcggg ggcggctgtc gccctggagg tcgagcatgc caaagacgag cgatcgcaat
     1021 acgggctcgg catgtaccca gccgatcgtc tgcaaggtgc gccagctctg cgcggcgaag
     1081 atggccttgt ggccgatatt gcgctgatcg cgcacggcaa cgcgccacag cggctccatg
     1141 gtctctgccg ctcccgagct gcggcaaagt gcggcaatgg ccgcgtcggc agcctccgga
     1201 tcccacactt ccatcgcccg gacaaattcc tccttcgctc gatgcgtctt ggggagccgg
     1261 cattcgtcga cctccgccaa gacccagtcc ccctccttga cgtcttgcgc ctgcgacgat
     1321 ttgaaattgt cgagtgccca catgaggggc aataaccgtt cggtcacggc cgccgattgg
     1381 ccgagaacgt gcgccgaatt aatgaccatg acggcgtgaa acttgaatcc gacgggccgc
     1441 ggcttgatgt tgcggattcc cgcgaggaac aatcctgaaa gcagctgatt gtaagagagc
     1501 cccttcttga gctgcgtgat cgccatttcg agggcgcggt cgcgcggagt atcctcgatc
     1561 agtcgcacaa caggttcgat ctcggggcgg aacttgacca tttcgggctt gagctgggct
     1621 tcttcaccct tgagcggggt gattgtcctc aagctggccc acggggctag ctcggcaccc
     1681 agaccgagcg ccgaagccat ttgcagaaac cgacgtcgac gaagtcctcg attcatcatc
     1741 gtgcgttctc cttcttcaca actggctgtt gctggcaggg aacggcgtgc gtcgcgccga
     1801 gcggatccaa gccggcaggc gataaacaac gtgctgccgt cattattgtg gcgccgcgca
     1861 acgtttggat agccggtcgc gcggtagagt gtagggaccg agccaatacg cactttttca
     1921 cgcgcgcatc atatcgttct cggagtgacc agacccgatg cccgagagtt tcgaacccct
     1981 gagagtgccg ttctcgacag ccgacgcaga cgctccggcc cttaagtaat gcgctgagcg
     2041 atcaactatg ctgcagctga ttgatcttgt tatagagggt cttcaagctg atgccgagct
     2101 cgcgcgaggc cgccggtttg ttaccttggt gtttctctag gatcgtctgg atgtatttca
     2161 tttcgacgtc acggagcgta gggcttccct cggggacctg gaaatgaggc ggtccgatcg
     2221 acgtaggcgc gctttgatgg gacttgctgc ggatgggaag ctgggttggg aggtgttccg
     2281 gcaggatcgg gctattgccg gcgaggatcg tggctcgctc gatcatgttc gcgagctcgc
     2341 gaatgtttcc cggccactca tgccccatga gaagctcgat ggactccggc acaatggttg
     2401 actcggttag accgcgacgc gatgcatgcc gggtcaggag atgcttggcc agcgcggcga
     2461 tgtcggttct tcgctcgcgc aggggaggca agaaaatctc gaaggtattg agccgaaaaa
     2521 agaggtcttc ccggaacagt tcgttcgcga ccatgtcgcg caagtcgcga ttcgtcgcgc
     2581 acagcactcg gacatcgacg cggaacggct cgttttcgcc gacgcgcctg atctcgccgg
     2641 attcgaggaa tcgcaacagt ttgacctgaa cgcttttgtc gagctccccg acctcgtcga
     2701 ggaagagcgt accgccattg gcgacctcga agagcccttt gcgattggta tcggcgccgg
     2761 tgaacgctcc cttgcgatga ccgaacagtt cgctctcgac caggttttcc ggcaatgcgc
     2821 cgcaattgac tggaatgaac gctcgatcag tacgacgact cttttcgtgg aggctgcgcg
     2881 cgacgagttc cttgccggtc ccggtctctc ccaggatcat gaccgacgca tcggtcgggg
     2941 cgatcgtatc gatcaaacgt cggacctgct gcatcgtcgg ggtctcgcca atcaagaggg
     3001 gcgacccttc agcggctttc aaacgggtct cgagtgcggc cgtcttgttg gccatgtcgc
     3061 gtcgctcggt gatccggttc aaaatcatct cgagctctgc ccacttgcag ggcttggtca
     3121 ggtagtcaaa tgcgccgagc cgcagcgcct gtacggccgt gtcgaccgtg gcatgcccgg
     3181 tcaaaatgat cacctgcgtt tcggggctta tctgcttgat ctggcccagg acctcgattc
     3241 ccgtgaatcc gggcatgcgc atatcgagga gcgccgcgtc gtacaaactc cgttcgagag
     3301 cccgaatcgc ggcggccccg tcggcgcaca ccgtcacttc atggcccagc cgtggcaact
     3361 ccatttgcat gaggtcgcga aggtgggctt catcgtccgc aaacaggatc cgcaatcctc
     3421 cctgacgcga cttttccatt tttcggtccc ttcctgagcc cgagggtggc tttgctcgac
     3481 tggatcatcc gactgatttg cgcaccacac acctctacag ggccgccgcc gcggactccg
     3541 agcgccgtcg aagctggctc gatcgcgcca gaccgtcttc acctatctcg gtcggatgga
     3601 tcggcagccg gactgtgaag gtcgatccca ggttctcgcc cgcgctgaaa gccataatct
     3661 cgccgtgatg ctgattgacg atcctgtgcg tgatcgacag gcccaacccg gtccccttgc
     3721 catcgcgccg cttggtgaaa aacggctcga agatgttctc gagcacatcg gggctcatcc
     3781 cgcagccgct gtcggtgaat accatctcgg ccataccttg attgtagcgg gcctcgatgc
     3841 gtattgtgcc gttcgagctc atcgattcga gcgcgttgac aatcagattc aggacgactt
     3901 gcttgatctc ctgggcatct acatgagcca tcacggcctc gcgcgggtga aacgtgatcg
     3961 tcttgccggt gtacttgccg atatggcgga tcatgtcgac gacgccttgg atcaaggacg
     4021 ccagatcgat ccgctcgcgt ttgatgtcgt tgcatctcga gaagtcgagc agcttttcgg
     4081 taatgttctt acagcgaaaa gattcttcct ggatcatctt caagtaattt gagacgaccc
     4141 gatgatcggg atcagcggaa gttctgagca atcgctcaag ccgatgatcg agagcctcgg
     4201 cgcagaaagc gatcgacgcc agcgggttgt tgatttcatg cgcgactccg gccgccaaaa
     4261 aaccgactcc cgccagccgc tctgaccgca ccagttgcct gcttcgctcc tggacctgcc
     4321 gctctaagtc ggcgtaagtc atgctgatct tggcggtcat atcgttgaac gcctcggcga
     4381 gatcttgcat ctcgtcgccg gatgcgaggt tgatcttata gtcgaacgag ccatgtgcca
     4441 cgtggcgaac cccgcgctgg aggaaacgaa gcggaaacag aacccatcgc tgaaacaaaa
     4501 acatcaggcc gcaaagcatg gcgagaacca tgagcgccga tacccagacg atcacccggc
     4561 tcgtttgata tttcaattgc gacaatctca agacctgcca gaaatcgctg aagagctttt
     4621 tggggagttg catgacggtg tcgtgcaagc gctcgatacg tgacttgatg ccggtggcgg
     4681 gctttacggg aggtaggagc tcgggatgat ctctcacgta gaaggatgtg ccgggcaaca
     4741 ggaaagcggc agtggtcccc gatggatcga gttcggtgcg aatggcggcc aggtcatcgt
     4801 cgataaggaa ggcaagaccg agctcgtctg tgccgttgtc gacccggctc ccctgattgg
     4861 tgtttttctt cagctggcga aaatagacca agagcgcgtc ctgagcgacg actacctgct
     4921 cgtgcaactc ttccatctcg gcgtcggtac cttcgcgcgg ggcgtgtagg cgaaataccg
     4981 actctatcaa ttcggcagac gcccccagct cacgtagctg gtcgacgagc gtcaaatagc
     5041 tcatgtggaa tgagtggagc ccgaagatcg aggcacccat caagatgagc atcatcccca
     5101 ccacgacgct caggccaaca atcagcttga gtcgaatggg ccatcggatc atgacgacat
     5161 ccttgtcgtg gcgacgagtc tgtagtgagg gagttccgcg agacgcggca tcgatgcgag
     5221 atcgatggcg tatcttacaa aactcccgat cgggctggaa ggcacaaatg gaggtgcccc
     5281 gctcgccgtg tacgccaacg gattctgcat tataggcgcg tgtagcgggc agtgtgggga
     5341 gcggcaccgc ctggactgcg ggcgcgtaga cctccggtcg ggcgccccgg cggggtcgga
     5401 gagccgcgcc gaacttcgcg cggatctcgg gcgcgggtct cttaccccgc cctccggcgc
     5461 gaccgtaggt ctccaaccgt gcggatggcg catcgctggc ggtcagaaca ttttgggaca
     5521 accaacttga tttgtcgagc agcttcggga ccgcgatgcg cgggcccggt ccgaggctgc
     5581 cctgccggcc cttctggcga acctggacga cgaggacctg atgggccgag tccacgccgc
     5641 gcgcggtcgc cccgctcggc gcggaggcga aggacgcgat gttcaagttg gtccgcctga
     5701 tctgggattt cggcccctat ttcggggtcg cccgatctgt gggagacatc gagcccactg
     5761 ccgccgtcgc cgtgccgccc ctggtcgccc actgggacgg cgcggatgcg c
//
LOCUS       DATAQL010001243         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3638905, whole genome shotgun sequence.
ACCESSION   DATAQL010001243 DATAQL010000000
VERSION     DATAQL010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638905"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..507)
                     /locus_tag="VHS97_21480"
     CDS             complement(136..507)
                     /locus_tag="VHS97_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450840.1"
                     /translation="MPSIGRGTLLQKDRVKILNSERELALGTKYSRVTVGFPMNIVTE
                     CKPFRRLAWATTVELDAEASAVSSDAALRTDLRQHLRRRWRRVGRFGCQHRSGDSRHG
                     VAVVIASRPPAAVATDVGFGS"
     gene            complement(626..1228)
                     /locus_tag="VHS97_21485"
     CDS             complement(626..1228)
                     /locus_tag="VHS97_21485"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX3450841.1"
                     /translation="MDYSIVGFGPVGQALARAFARKGIEVAVATTREPAAIASTTRSI
                     GPAIVPKSLKDALASDVILLAVPFWAHRDVAQGLPSWLGKIVIDVTNAYGVPLADLGH
                     LPSSKVIANALHGARLVKAFNHLAAGVLAQDPTVGEGRRVIFLSSDDEGAIATVAALV
                     EQLGYAPVSLGKIAEGGQLVQGCDKSWGPLIFQDLFKKLR"
     gene            complement(1388..>1553)
                     /locus_tag="VHS97_21490"
     CDS             complement(1388..>1553)
                     /locus_tag="VHS97_21490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3450842.1"
                     /translation="VLDEEAKRQFESLIPRGKMGRPEEIATVALFLASDDSSFVNGVE
                     LSVDGGTSAI"
BASE COUNT          295 a          513 c          450 g          295 t
ORIGIN      
        1 cgcaatgacc ccgtagggtc gactgcggcg gtctaggaaa ggcttcatga accgaagccg
       61 cggcgctcac ccatgcggta tctgtccgga gctggatgtt cagctgtcat ccactgcccg
      121 ctgctgcaca tgcgctcatg acccaaagcc gacatcggtg gccactgcag cgggcggccg
      181 actggctatc acgacggcga cgccatgccg actgtcaccc gatcggtgct ggcacccgaa
      241 tcggcccacc cgccgccaac ggcggcgtag atgttgacga aggtcggtcc tcagcgcggc
      301 gtccgaactg accgcgctgg cttcggcgtc aagctcgact gtcgtcgccc atgcgagcct
      361 gcggaagggc ttgcactccg tgacgatgtt catggggaat ccaaccgtca ctcggctgta
      421 cttggtcccg agtgccagtt cccgctcact gttcaggatc ttgacccgat ctttctggag
      481 gagagtacct cgaccaatgc tcggcatcga gcagcagttt ccagaccacc tcaggcggcg
      541 ccttcacgtc gatgtcgttg accgcgtata tggccgacgt tttcggatca taccgctcgg
      601 gccagatcac atggtcatac atcggtcacc gcaacttctt gaacaggtct tggaagatga
      661 ggggccccca actcttgtcg cacccctgca cgagctgacc gccttccgcg atcttgccga
      721 gactgaccgg cgcatagccg agctgctcga ctagggcggc gaccgttgct atcgcgcctt
      781 cgtcgtcgct cgagagaaag ataacccgac ggccctcgcc gacggtcggg tcctgcgcaa
      841 gcaccccagc cgctagatga ttgaaggctt tgacaagtcg tgcgccatgc aaggcgttgg
      901 cgatcacctt cgatgacggc aggtgtccca gatcggcaag cggaacgcca tacgcgttag
      961 tgacatcgat aacgatcttg cccagccagc ttggaagccc ctgcgccacg tcgcggtgcg
     1021 cccagaaggg gaccgccagc agaatgacat ccgacgcaag cgcgtccttg agcgacttgg
     1081 ggacgatggc cggtccgatt gagcgcgtgg tcgacgcaat tgctgcgggc tctcgcgtcg
     1141 ttgctaccgc cacctcaatt cctttgcgag cgaaggctcg cgccagggcc tgcccaacgg
     1201 ggccgaaacc aacaatcgaa tagtccatcg cgtttctcct gtacgagtgc ccggccttct
     1261 ttccggctgc cggcctgccg gccgtgaggt attcctcgcc aaagcatcac aagcttgggc
     1321 agccaatgcg ggggtactga tggatataac cagtcgacag tgcccccaca cgcttttcgt
     1381 cgcgatttca gatggccgag gtgccgccgt cgacggacaa ctccacccca ttcacgaagc
     1441 tcgaatcgtc tgaagcaaga aacagcgcga ccgtcgcaat ttcctcagga cgacccatct
     1501 ttccccgcgg gatcagggat tcgaactgcc gcttcgcctc ctcgtcgaga act
//
LOCUS       DATAQL010001244         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3028293, whole genome shotgun sequence.
ACCESSION   DATAQL010001244 DATAQL010000000
VERSION     DATAQL010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3028293"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VHS97_21495"
     CDS             <1..891
                     /locus_tag="VHS97_21495"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450843.1"
                     /translation="RFRLATQALEVLGVRQDQIDTIASDNRADVLLPAVAPIDGLVIK
                     KTAYQGQYVPEGTLLFEIADLRHVWVDAQVFEEQLALVRVGQPVTATVPAFPGEEFRG
                     HVEQLAPALDPATHTLAVRFGLDNPRDRLRPGMLATVALDVAGRVTTLARGATCPVTR
                     VRLGTMGPAIPVEVQGRRVSVCCEACVPKLKAAPQKYLEELGPRDTATPDRVMSVPES
                     AVVDTGTKTIVYVEASPGVFEGRAVVLGARSGDSFPVLDGLAPGDRIAAAGAFLIDAE
                     TRLNPPSRSVGLSAAADDPR"
     gene            complement(967..1485)
                     /locus_tag="VHS97_21500"
     CDS             complement(967..1485)
                     /locus_tag="VHS97_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450844.1"
                     /translation="MRAILWKLRPAALLTALGWLFATPEAHAQSISSIRSLGGYGATT
                     TSPMASMSSSSPMIPYAGSFAGFMPYRMASGASSPLSFSPRGSSAPELTRTPFRLSPM
                     SSGMGAGLRPDSGLFAPLGSQGAMRSGGGLLSQPMGGGTNTSVMPPNFGYPFYQPPSL
                     FSPVSSGSGMSM"
BASE COUNT          339 a          578 c          564 g          338 t
ORIGIN      
        1 cggttccggc tcgcgaccca ggcgctcgag gtcctgggag tccgccagga tcagatcgac
       61 acgatcgcct cggacaaccg cgccgacgta ctcctacccg cggttgcgcc gattgacggt
      121 ctcgtcatca agaaaaccgc ctatcagggc cagtatgtcc cagaagggac gctcctgttc
      181 gagatagccg atctgcgcca cgtctgggtc gatgcccagg tcttcgagga gcaactggca
      241 cttgtccgcg tcggccagcc ggtcacggcg acggttccgg cgtttcccgg cgaggagttc
      301 cgaggccatg tcgagcagct cgctcccgcg ctcgacccgg cgacgcacac cctcgcggtg
      361 cggttcggtc tcgacaatcc cagagatcgt ctccggcccg ggatgctcgc gaccgtggcc
      421 ctggacgtcg ctggtagggt gacgaccttg gcccgcggcg cgacgtgccc ggtgacccgc
      481 gtccgattgg gaacgatggg gccagcgatc ccggtcgagg tccagggccg tagggtctcg
      541 gtctgctgcg aggcctgcgt cccgaagctc aaggccgctc cccagaagta cctagaggag
      601 ctcggccccc gcgacaccgc gacaccagac cgcgtaatga gcgtgcccga gtcggccgtc
      661 gtagataccg gcaccaagac gatcgtgtat gtcgaggcca gtcccggagt attcgaggga
      721 cgagccgtgg ttctgggtgc acgctcgggc gactcatttc ccgtcctcga cggcctcgcc
      781 cccggtgacc ggatcgccgc cgccggcgcc ttcctgatcg atgcagagac gcggctcaat
      841 cctccttcac gctccgttgg tctgagcgcc gcggcggacg atcccaggtg acggagcaga
      901 aaagaggagc aggcgatccc ctgcggccaa cgacgtgaat cgtccttgtc aaacgcctct
      961 ccaatctcac atcgacatgc cagagccgga agagacgggc gaaaacagac taggcggctg
     1021 atagaaagga tagccgaaat tgggcggcat gacgctcgtg ttggtgccgc cccccatcgg
     1081 ttgactcaag agtccaccgc ctgatcgcat ggcgccctgg gagcccaagg gagcaaataa
     1141 tccgctgtct ggcctcaaac ctgcacccat tccgctcgac atcggcgaca gacgaaacgg
     1201 cgtccgcgtc aattccggcg ccgagctgcc gcgcggcgag aacgagaggg ggctgctcgc
     1261 gccgctcgcc atgcgatacg gcatgaaccc ggcaaaactg ccggcatagg ggatcatggg
     1321 gctgctcgag ctcatgctgg ccatcgggct ggtcgtagtc gctccgtagc cgcccaagga
     1381 acgtatgctg ctgatgcttt gtgcgtgcgc ctcgggagtc gcgaagagcc agcccagcgc
     1441 ggtaaggagg gctgccggcc tgagtttcca cagaattgcg cgcatgtctc aacccccccg
     1501 tggtacttgc cgatgactct ggtgattggt tgaccagcgg ctcccattcg agagaacgag
     1561 taagataaag actaagatta tgaagaggag taagagtaag agcacgacta cgattaggac
     1621 ttaattcgtc gcattgatcg tcaaatctta atcttcatcc tgctcttgat caggacataa
     1681 tagactgcgg agatgtcgct tctgagcttc cgtggcatcg gatcggatct ggagcttcca
     1741 gttgccctct ggctcttttt tcttgggctc gccttttttc gatgaaccct cgccggccgc
     1801 atctgagctt atctccggt
//
LOCUS       DATAQL010001245         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2111945, whole genome shotgun sequence.
ACCESSION   DATAQL010001245 DATAQL010000000
VERSION     DATAQL010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111945"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>2509
                     /locus_tag="VHS97_21505"
     CDS             66..>2509
                     /locus_tag="VHS97_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450845.1"
                     /translation="MSKRQPAIYYRSTTAAFWPLSSRPRPVSYLWTGTRAEPARLNLT
                     KDLVFQFEARTADGRFLPKHRYKIEVVEDRAPRVSFEEPDVALEVHPVAEVLNRIRVG
                     DDFGLTKAGIVFQFNNGDEQALVVKDFTAEPPKARIAASLQEMLLLEKLAVSPTDSLT
                     YYAYAEDNNPAGPRRTETDLRYLDIRPFKREYKLGESASGIEAEGELASLAELIARQR
                     FNLNRANRLAKHKPTDKTIAEDPLKIAGFEETLAGMTRELTEGVEGIVGQRIEPLHAA
                     EEAMLAAVAALDHGQNARSPGHMSDALRHLIKARDTLRIGIGQDQETRQAMRSFDRMQ
                     AQKIRKPKKDEQEAEEIAEELEALAREEDFVYETLGGILMEQQKGENGKGGETDKGKE
                     DADKTSERAEKKDEAQKSAKNSKEQKGAEGSAGHGQGRGQAGKKGEPDEGEKTGEPRN
                     SKRREAVEKQEKIVDRARELEEKLKKLEVASDLAKLRMNKAAETAEKTSSALSRGNTK
                     EATESAKAGAAMLHELARQVKGEIARDVAQELAMARDLADELAAREAEIGQMSNESPG
                     PGADQGAPDGQPGSGEKGQPGPPGKNDQGTSGLDGKGIAGRGGWGDLTDAERLERLEE
                     AAKTLEHWLKDASLRAEGQSAERIRDLLEERAATRVVERMERIGELYLGGQVPAARRQ
                     ANELSKVLELLARQLEILHRTIVAPELTAMVDLDRRVAELTAKLKTITTDAEIAEWRR
                     LAADLVRDLEKAGLTDAATALVNAFEAGGWHWGVGVEHFRTAPAGFTIALQSVSSLLQ
                     AKIQNLILKDMASARD"
BASE COUNT          572 a          731 c          838 g          368 t
ORIGIN      
        1 aggggccgcg actgccaacg tgacttttcc gctcaacgag ttgcccgaag gaatgtggcg
       61 tgggcatgtc gaagcgtcag ccggcgatct attaccgttc gacgaccgcc gctttctggc
      121 ctttgtcgtc tcgcccccgg cccgtctctt acttgtggac ggggacccgg gccgagcccg
      181 cccgtctcaa tttgaccaag gacctcgtgt ttcagttcga agcccggacc gccgatggcc
      241 ggttcttgcc caagcaccgt tacaagatcg aggtcgtcga agaccgcgcg ccgcgtgtgt
      301 cgttcgagga gcccgacgtc gcgctcgagg tgcatcccgt cgccgaggtc ttgaaccgca
      361 tccgcgtggg agatgatttc ggtcttacca aggccgggat cgtcttccag ttcaataatg
      421 gcgacgagca ggcgctcgtc gtcaaggact tcacggcgga gccacccaag gcccggatcg
      481 ccgcgtcact ccaggagatg ctgctgctgg aaaagctcgc cgtgtcgccg acggatagcc
      541 tgacctacta cgcctacgcc gaggacaaca atccggctgg gcccaggcgg accgaaactg
      601 acctgcgcta cctcgacatc cgcccgttca agcgagagta caagctgggg gaaagcgcca
      661 gtggcatcga ggcggaaggg gagctcgcga gcttggcgga attgatcgcc cgccagcggt
      721 ttaacttgaa ccgagccaac cggctggcca agcacaagcc gaccgacaag accattgccg
      781 aagacccgtt gaagatcgcc ggctttgaag agacgctcgc cgggatgacc cgcgagctta
      841 cggagggagt cgaagggatc gtcggtcagc ggatcgagcc cttgcacgcg gctgaagagg
      901 ccatgcttgc cgccgtcgca gccctcgacc acggccagaa cgcccgatcc ccgggccaca
      961 tgagtgacgc ccttcgtcac ctgatcaagg cccgcgatac gcttcggatc ggcattgggc
     1021 aagaccagga gactcgacaa gcgatgcgca gcttcgaccg catgcaggcc cagaaaattc
     1081 gcaagcccaa aaaggatgag caagaggccg aggaaatagc cgaggaactc gaggcactcg
     1141 cccgggagga agacttcgtc tacgagaccc tgggtggcat cctgatggaa cagcaaaagg
     1201 gcgagaacgg taaggggggc gaaaccgaca aaggcaaaga ggacgcagac aagacgtcgg
     1261 agcgcgccga aaagaaagat gaagcgcaga agagtgccaa gaactcgaaa gaacagaaag
     1321 gggcagaggg ctcagcggga cacggccagg gtagggggca ggccggcaag aaaggtgagc
     1381 cagatgaagg ggaaaaaacg ggcgagccca ggaatagcaa acgccgcgag gccgtcgaga
     1441 aacaagagaa gatcgtggat cgcgcacgcg agctggaaga gaaactgaaa aagctcgaag
     1501 tagcctcgga cctcgccaag cttcgcatga acaaggctgc cgagacggcg gaaaaaacct
     1561 caagcgcgct gtctcgtggc aacacgaaag aagcgaccga aagtgccaag gcaggcgccg
     1621 cgatgcttca tgaactggct cgacaggtca agggcgagat cgcccgtgat gtcgcgcaag
     1681 aactggcgat ggcccgcgac ctggccgatg agctggccgc gcgcgaggcc gagatcggcc
     1741 agatgtctaa cgagagtcca gggcctggcg cggaccaagg cgctcctgat ggccagcctg
     1801 gttcgggcga gaaagggcag ccaggtccgc ctggcaagaa cgatcaagga acatctggat
     1861 tggacgggaa aggcattgcc ggccgtggtg gttggggaga tttgacagac gccgagcgac
     1921 tcgagcgctt ggaggaagca gccaagaccc tggagcattg gctgaaagat gcgagcttac
     1981 gcgccgaggg acagtcggcc gagcggatcc gcgatttgct cgaggagcgg gccgctaccc
     2041 gggttgtcga gcgcatggag cgaattggcg agctctactt gggtggccag gtgccggcgg
     2101 ctcgacgcca ggcaaacgag ctatcgaagg tgctcgagtt gctcgcgcgt cagcttgaaa
     2161 tcctgcaccg caccatcgtc gctccggagc tcaccgcgat ggtcgatctg gacagacgcg
     2221 ttgccgagct gacggcgaag ctcaagacca tcacgaccga cgcggagatc gccgagtggc
     2281 gtcgcctggc tgccgatttg gtgcgcgacc ttgagaaggc cgggctgacg gacgccgcga
     2341 cggcgctcgt caacgcattc gaagcgggcg gctggcattg gggtgtcgga gttgaacact
     2401 tccggaccgc tcctgcaggt ttcacgatcg cgcttcagtc cgtaagcagc ctgctgcaag
     2461 ccaagatcca gaacctgatc ttaaaagaca tggcgtcggc ccgcgatga
//
LOCUS       DATAQL010001246         4572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_279288, whole genome shotgun sequence.
ACCESSION   DATAQL010001246 DATAQL010000000
VERSION     DATAQL010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279288"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VHS97_21510"
     CDS             complement(<1..273)
                     /locus_tag="VHS97_21510"
                     /inference="COORDINATES: protein motif:HMM:NF021988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEX3450846.1"
                     /translation="MPASWEVLLEQARSAFADRFGGEPAALGCAPGRVELLGNHTDYN
                     GGLVMAAAIDRFTVVVGRRSRDRNATVRSVNFGQTDTFLVDAIERTE"
     gene            complement(416..1609)
                     /gene="metK"
                     /locus_tag="VHS97_21515"
     CDS             complement(416..1609)
                     /gene="metK"
                     /locus_tag="VHS97_21515"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX3450847.1"
                     /translation="MPQQDRYLFTSESVSMGHPDKMADQISDGILDAILQQDPYARVA
                     CESLLTTGLVCLAGEITTKAMVDYPAIVRQIVRDIGYTSSDMGFDAGTCAVMVALGKQ
                     SPDIAQGVDEDKTSGKDIGAGDQGMMFGFACNDTEELMPLPIALAHRIINRITVLRQD
                     GTIKWLRPDAKSQVTIEYDGYTPIRVDTVVVSTQHAPDVSHAEIVKTITEQVIQPVLP
                     TDLINGSPTYHINPTGKFVIGGPHGDSGVTGRKIIVDTYGGMGRHGGGAFSGKDPTKV
                     DRSAAYMARYVAKNIVAARLAERCEIQLAYAIGVSEPVSVHVDTQGTGTLPDSMIAEL
                     VRKHFRLNPSGIISHLDLRRPIYRKTASGGHFGRSEPEFSWEKTDKAHVLREAANSFA
                     PSTMA"
     gene            complement(1845..2753)
                     /locus_tag="VHS97_21520"
     CDS             complement(1845..2753)
                     /locus_tag="VHS97_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450848.1"
                     /translation="MPKTPPAYRLVIFEPIAEPAELRDLVCRVTGMHPTDAVQWLARA
                     PGTWPQPLDETTVRGLLDGFYEAGVAAEAWRTDLFPDLSPPRTIHRAACMKEGFRIEG
                     LRGEPTHWVPWDRIELVCAGRISTEDQFRNVQPPRWPSTVVSGIRALALMKPNPTSRK
                     GRASRIPRDPVGEAVIVRRDPRIAFRVVENHMNYAYLGNRLSHSAADNFPVFVADLCA
                     RADAAYITDSTRSLLENREPGEHEFPSSLALLEYATHRLLWSWYRRDRDAQSGRRAAG
                     ETSDPEIDNSPGTDVEPDEDFGEPRG"
     gene            complement(2798..3529)
                     /locus_tag="VHS97_21525"
     CDS             complement(2798..3529)
                     /locus_tag="VHS97_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3450849.1"
                     /translation="MATISTAKRPPRKTWTVADLYHRFGPIPFERIRQNPSPGSGTVD
                     DVVRLNDHEDRLYELVDGILVEKTVGLVESLIAITIGTLINNFVRPRGLGLVAGEGGT
                     LQLDINLVRIPDVSFISWDRLPGGEVPQEPVPLLVPDLAVEVISRGNTRKEMDEKLQE
                     YFEKGVRLVWYVRPRSRVVDVYTGPDHFARLTASMQLDGGEVLPGFSVQVGELFELPK
                     RPTANGQRKKNEPRPGKKKGPRRGR"
     gene            3778..>4572
                     /locus_tag="VHS97_21530"
     CDS             3778..>4572
                     /locus_tag="VHS97_21530"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3450850.1"
                     /translation="MRQIGTVPKRLDVKVLADYLLTLGMKTRVDEAADDWTVWIYHED
                     HFPRAREELQAYVSRPDDPRYQSAVNAADEIRRKERELDQQFRKNYREVSDIWASPGL
                     RRRPLTMALVAICVVVFVFQRSREGRSLERRLWFSNTQVSATGGEKDSGLSDILHGEV
                     WRLVTPIFMHGNPIHIFFNMWWLVSLGTMIEARRGTLRLAGLVLIAAIVSNFGQYIWM
                     ERIDPGEPHKFLGMSGVVYALFGYIWMKSYYEPEQGLFMHPNNVNLM"
BASE COUNT          876 a         1336 c         1438 g          922 t
ORIGIN      
        1 ttcggttcgc tcgatcgcat cgaccagaaa agtatccgtt tggccgaaat tgaccgagcg
       61 gaccgttgca tttctgtctc tcgaacggcg gcccacaacg acggtgaacc gatcgattgc
      121 cgcggccatg accagaccgc cgttgtagtc ggtgtggttg cccaggagct cgacccggcc
      181 gggcgcgcag ccgagagccg caggttcgcc accaaaccgg tcggcaaatg ccgaccgagc
      241 ctgctccaag aggacttccc acgaggcggg catgaatcat acctcgcgcg ggggcaaaag
      301 ctacgtttga ggacgggagg gcgaggctcc gcccgagccg gcaggggatg cgaagaggct
      361 cggcaggatc ctcgccctcc caaaaaacag atcgaatccg cctgcgcgac gtatatcagg
      421 ccatcgtcga tggcgcgaat gaattcgctg cttcgcgcaa gacgtgggcc ttgtcggttt
      481 tttcccagga gaactcgggc tccgaacggc cgaagtggcc tccgctggcc gttttgcggt
      541 agatgggccg ccgcaggtcg aggtgcgaga taatacccga ggggttgagc cggaagtgct
      601 tgcgcaccag ctcggcgatc atcgagtccg gcaacgtccc ggtcccttgg gtatcgacat
      661 ggacgctgac cggttcagag acgccgatgg cataggcgag ttgaatctcg catcgctccg
      721 ccaatcgcgc cgccacaatg tttttagcta catagcgggc catgtaggcc gccgaccggt
      781 cgaccttggt gggatctttg ccgctgaacg ctccaccgcc atgccgaccc atgccgccgt
      841 aagtgtcgac gatgattttg cggccggtta ctcccgaatc gccatgggga ccaccgatca
      901 cgaatttccc cgtcgggttg atgtgataag tgggtgagcc gttgataaga tcagtcggca
      961 acacaggttg gataacctgt tcggtgatgg tcttgacgat ctcggcgtga ctgacgtcag
     1021 gggcatgttg agtggagaca acgacggtgt cgacgcggat gggtgtgtat ccatcgtatt
     1081 cgatggtcac ctggctcttg gcatcggggc ggagccactt gatcgtgcca tcctggcgca
     1141 aaaccgtaat ccggttaatg atccggtgtg ccagcgcgat gggcagcggc ataagctctt
     1201 cggtgtcgtt gcaggcgaag ccaaacatca tcccctggtc gcccgcgccg atgtctttgc
     1261 cactcgtctt atcttcatcc acaccctgag cgatgtctgg cgactgtttg cccagggcga
     1321 ccatgacggc gcacgtcccc gcgtcgaacc ccatatcgct cgaggtgtag ccgatgtcgc
     1381 ggacaatctg gcggacgatc gccgggtagt cgaccatcgc cttggtcgtg atttcgcccg
     1441 ccaggcagac gagtccagtc gtgagcaagc tttcgcaggc cacccttgcg tacggatcct
     1501 gctgcaaaat ggcatcaagg atgccatcgg aaatctggtc ggccatcttg tcgggatgac
     1561 ccattgagac ggattcgctg gtgaataaat agcggtcttg ttgcggcacg ggagcgtatc
     1621 tcctaccggt cgagtatcag cgttttcgcc gaccgcccga cccaagatga gagggtgacc
     1681 gaccggctgg tgtgagtcaa ttttcgcgaa acagttgccc tttaagaaat cagagtctaa
     1741 caaaagggta cgctcttgaa aagaatcgct caactctcgt tgccgacaac catcgccgcc
     1801 gttgggatca gaatattggc aggagccaaa cgtctgtcca agcgttatcc gcggggttca
     1861 ccgaaatctt cgtccggctc gacatctgtg cctgggctgt tgtcgatttc cgggtcggac
     1921 gtttctccgg cggcacgcct gccggattgg gcgtcgcggt cgcggcgata ccagctccac
     1981 aataatcggt gggtcgcgta ttccaaaaga gccagtgagc tggggaactc gtgctcgccg
     2041 ggttcacggt tttccagcag cgatcgggtg gaatcggtga tataggccgc gtcggcccga
     2101 gcgcagaggt cggcgacgaa cacggggaaa ttgtctgcgg cagaatggct gaggcggtta
     2161 cccaggtagg cgtagttcat gtggttttcg acgacgcgaa acgcgatgcg cgggtcgcgc
     2221 cgaacgatga ctgcttcgcc gaccggatcg cgggggatgc gtgatgcccg gcctttacga
     2281 ctggtcgggt tgggcttcat caaggccagg gcgcggatcc ccgagacgac ggtcgatggc
     2341 caacggggtg gctgaacatt gcgaaactgg tcttccgtgg agatcctccc cgcgcagacg
     2401 agctcgatcc tgtcccacgg aacccagtgc gtcggttccc cgcgcaggcc ctcgatgcgg
     2461 aaaccttcct tcatgcacgc ggcgcggtga atcgtccggg gtgggctcaa gtccggaaac
     2521 aagtcggtcc gccacgcctc agccgccacg ccggcctcat aaaaaccatc caagagaccg
     2581 cgcaccgtcg tctcatccag cggctgcggc caggttcccg gcgcgcgggc cagccactgg
     2641 acggcgtcgg ttgggtgcat tccagtcacg cggcaaacca ggtcacgcag ctccgccggc
     2701 tcggcaatcg gctcgaaaat caccaggcga taggcgggcg gcgttttggg catgatcggt
     2761 gcagactccc ggctgtgggc acaaagtgcg tgacacgtta acgaccgcgc cgcggcccct
     2821 tctttttccc tggtcgcggc tcgtttttct tccgctggcc attggcggtg ggtcgcttcg
     2881 gtaattcaaa cagctcgccg acctggacgg agaatccggg aaggacctcg ccgccgtcga
     2941 gctgcatcga ggccgtcagc cgcgcaaagt ggtccggtcc ggtgtagacg tccacgaccc
     3001 gactccgcgg ccgaacgtac cagaccaggc ggacgccttt ctcgaagtat tcctggagct
     3061 tttcgtccat ctctttacgg gtgttgcccc ggctaatgac ctccacggcc aagtcgggaa
     3121 cgaggagggg aactggctct tggggtacct ccccgccggg aagtcggtcc cacgaaatga
     3181 acgagacgtc gggaatccgc acgaggttga tgtcgagctg gagtgttccc ccttcaccgg
     3241 ccaccagtcc cagtccccgc ggcctgacaa agttgttgat gagagtaccg atagtaatgg
     3301 cgataaggga ttccacgagt cccacagttt tctccaccaa gatgccgtca accagctcat
     3361 agagccggtc ctcgtggtcg ttgagccgga caacgtcgtc gacggtcccg gagcccggag
     3421 acgggttctg gcgaatgcgc tcaaatggga tcggcccaaa gcggtggtac aggtcggcga
     3481 ccgtccatgt cttgcgtggg ggtcttttgg ccgtgctgat cgtcgccatg gcgaagtctc
     3541 tttgttggca gggaggtgag cccagatcat cgtatcatgc ccgtcacagc tagccaatcg
     3601 acggcaagag ccttgagacg aaaggcaata ctgggatgat tagggcgatt cgaccgtgtg
     3661 tgcttttggc gctcttgtgg tagtctggca gaggtctcgg ggaacgcggc gacttaccgg
     3721 ggccgggtcg attctgctag ggcgcgtcgc cttgatcgaa ttcgagggga tggaccgttg
     3781 cgccagatcg gaacagtgcc caagcggctc gacgtcaaag tactcgccga ctacctgctg
     3841 acattgggga tgaaaacgcg cgtcgacgaa gcggccgacg actggacagt ctggatttat
     3901 cacgaagacc actttccgcg cgcccgcgag gaactgcagg cctacgtgag ccgccccgat
     3961 gacccccgct atcaaagcgc cgtgaatgcc gcggatgaga tccgccgcaa agagcgagaa
     4021 ctcgaccagc agtttcgcaa gaactatcgc gaagtctccg atatctgggc gtctccgggc
     4081 ttgcgccggc gcccgttgac aatggcgctc gtggccatct gtgtggtcgt ttttgtgttt
     4141 cagcgatcac gcgagggccg ctcgctggaa cgtaggctgt ggttttccaa cactcaggtg
     4201 agcgcgaccg gaggggaaaa agacagcggc ctgagcgaca ttctccacgg cgaggtctgg
     4261 cgtcttgtaa ctccgatatt catgcatgga aaccccattc acattttctt taatatgtgg
     4321 tggctcgtct cgctggggac catgatcgag gcccgccggg gaaccctccg gctcgccgga
     4381 ttggtcttga tcgcagcgat cgtgtcgaac tttggccagt atatttggat ggaacgaatt
     4441 gaccccggtg agcctcacaa gttcctgggc atgtcggggg tggtctatgc cctgttcggg
     4501 tacatctgga tgaagagtta ttacgaaccc gagcaggggc tcttcatgca tcccaataac
     4561 gtgaatctca tg
//
LOCUS       DATAQL010001247         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_586059, whole genome shotgun sequence.
ACCESSION   DATAQL010001247 DATAQL010000000
VERSION     DATAQL010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586059"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VHS97_21535"
     CDS             <1..213
                     /locus_tag="VHS97_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450851.1"
                     /translation="GRKSSSRGGATVKTCSHCLQRTGLPMYTRRTLKVAWQLGQTTTI
                     LSARSDGGTDGADDCAPEPLGIGSFV"
     gene            complement(463..>2275)
                     /locus_tag="VHS97_21540"
     CDS             complement(463..>2275)
                     /locus_tag="VHS97_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX3450852.1"
                     /translation="VIFLWPVQEVRVWIRPRPRLVHTAAHSHAATTMARADVEEWERP
                     SVLPATATAKSVSIHPPIPVTTANDAPPHRLQQPAITQDRVTSTARDLAPLPQVEPGS
                     PFQLPPIEMLEPPTPAPIQEHEAKVHARAMLLERTLLDFGYQVRVVQIDTGPVITQFE
                     IELEAGLRVSRIMSLADDLAIALAVPAVRIVAPIPGKTTVGIEVPNDHRTVVRLGDVM
                     TGVGSRTQKCRIPLFLGKDVKGQPLVFDLAEMPHLLMAGRTGTGKSVCLNAMILSILM
                     TKGPDEVKMILIDPKKVELSQYKRIPHLMHPVVTDMKKAESILSWACDKMEERYEFLR
                     RAEVRNIDTYNKLGPDEIFARLQPEDDEERIRIPTYMPYIVIIIDELAEVMMTAEKEV
                     EQHIVRLAQLARAVGMHLLLATQKPVVDVVTGLIKGNMPARIAFQVTNRSDSRVVLDE
                     LGAERLLGNGDMLFLIPGTSHIIRAQGTYVNDTEINRVRQYLERYPVEFSREIMQLQV
                     AGGPGGKERGAALKERDDMYEPAIEIVIREGRGSCSLLQRALGIGYGRAARLIDFMAE
                     DGIVGEYKSGSAREVLYSWEEWEALKNGGEPKGEAAA"
BASE COUNT          400 a          668 c          749 g          458 t
ORIGIN      
        1 ggtcgcaaat caagttcgag gggtggcgcc accgtgaaga catgctcgca ctgcttgcaa
       61 cggaccgggt tgccgatgta tacccggcgg actctcaagg tcgcttggca gctcgggcag
      121 accacaacga ttttatcggc tcggtccgac ggcggaaccg acggagccga cgattgcgcg
      181 ccggaaccct tggggatcgg ttcgttcgtc tgatcgacgc cgaaacgctg gtcggatacg
      241 ttcaaagaac cggcccctga tgcatcaacg aggacggatt ggcatgtggc ccgtggtcgg
      301 ttggtgactt gcgcgagatc tcgaccactc ataaattgtt ccatgataat tcgcgggatt
      361 caagcgggcg ggcaaaaacg cggcgctcga acgtgcgctt attccggtgg tcaggccgcc
      421 ctagcacgcg ggcagctgtt ctgcacgcag ctttttcggc cttcacgcgg ccgcctcacc
      481 tttgggctcg ccaccattct tgagggcttc ccattcttcc caggagtaga ggacctcgcg
      541 ggccgacccg cttttgtatt cgccgacgat gccgtcttcg gccatgaagt caatgagccg
      601 ggcggcccgg ccgtagccaa ttccaagcgc gcgctggagg agcgagcatg agccgcgtcc
      661 ttcgcggatg acgatctcga tcgccggttc gtacatatcg tcgcgttcct tgagggctgc
      721 cccgcgctcc ttgccgcccg gtccgccggc gacctgaagc tgcatgatct cgcggctgaa
      781 ctcgacgggg tagcgctcga gatactggcg gactcgattg atttcggtgt cgttcacata
      841 agttccctgg gctcggatga tatgcgaggt gccggggatc aagaagagca tgtcaccgtt
      901 gcctaaaagc cgctcggcgc ccagctcgtc gaggaccacg cgcgagtcgc tgcggttggt
      961 gacctgaaag gcgatccggg ccggcatgtt gcccttgatg aggcccgtga cgacgtccac
     1021 gaccggcttt tgcgtggcca gaaggagatg catccccacc gcccgcgcaa gctgagcaag
     1081 cctcacgatg tgttgctcga cctctttctc ggccgtcatc atcacttcgg ccagctcgtc
     1141 gatgatgatc acgatatagg gcatgtaggt ggggatgcgt attcgctctt cgtcatcctc
     1201 gggctggagc cgcgcgaaga tctcatcggg gccaagcttg ttgtaggtgt cgatgtttcg
     1261 cacctcggcg cggcggagaa actcatagcg ctcctccatc ttgtcgcagg cccacgagag
     1321 aatggactcc gcctttttca tgtcggtgac gaccgggtgc atgaggtgcg ggatccgctt
     1381 gtactgcgag agctcgacct tcttggggtc gatcaggatc atcttcacct cgtcgggccc
     1441 tttggtcatc aagatcgaga ggatcattgc gttcaagcag accgatttac cagtaccggt
     1501 gcggccggcc atcaaaaggt gcggcatctc ggccaggtcg aagacgagcg gctggccctt
     1561 gacgtccttg cccaggaaga gcggaatgcg gcatttctgg gttcggctgc cgacgccggt
     1621 catcacgtct cccagtcgga cgacggtgcg gtggtcgttg gggacttcaa ttccgacggt
     1681 ggttttgccc ggaatcggcg cgacgatccg cacggcgggc accgccagtg cgatcgccag
     1741 gtcgtcggcc aggctcatga tgcgcgaaac acgcaagccg gcttcgagct cgatctcaaa
     1801 ctgggtgatc acggggccgg tatcgatctg gaccacgcgg acctggtaac cgaaatcgag
     1861 cagcgtccgc tcgagaagca tggcccgggc gtggaccttg gcttcgtgtt cctggatcgg
     1921 ggcaggagtc ggtggctcga gcatttcgat cggcggcaac tggaacgggg agccaggttc
     1981 gacctgaggc aggggggcca gatcgcgcgc agtcgaagtg acgcggtcct gagtgatcgc
     2041 tggttgttga agtcggtgag gcggagcgtc attcgcggtc gtcacgggaa tcggcggatg
     2101 gatagacacc gacttggcgg tggcggtcgc tggtagaacg ctgggtcttt cccactcctc
     2161 cacgtcggca cgggccatcg tggtggcggc atgagagtgg gccgcggtgt gcaccagccg
     2221 cggacgcggt cggatccaga ctctgacctc ctggaccggc cataagaaaa tgacg
//
LOCUS       DATAQL010001248         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2263402, whole genome shotgun sequence.
ACCESSION   DATAQL010001248 DATAQL010000000
VERSION     DATAQL010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263402"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VHS97_21545"
     CDS             complement(<1..746)
                     /locus_tag="VHS97_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450853.1"
                     /translation="MNRRVWLRSLCAGFVASPSNRGRPSLAQEVPYRSPYSLQFRHLL
                     PDLGVGFDLPPWNNPHLESEVPARDWYSPRHERMLGAWGPRARQYPPAPDLAQRPATW
                     MQDRVILAGSRWIGYPYQHHHIPDWDPPASWPWHRVAYGRNSKGIDCSNFSSFCYNYA
                     LGVKLDTGIRQQAKRREVRGPGGRGILTVETIERAPYNALVAMLLPADLLYIRNDKVR
                     IAHVILWLGVVGASPNRVPLILDVTGGNHE"
     gene            complement(1000..>2337)
                     /locus_tag="VHS97_21550"
     CDS             complement(1000..>2337)
                     /locus_tag="VHS97_21550"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX3450854.1"
                     /translation="GFRPDELEEISLLEGQDRRFRLIDADAKSAILQAPASVEPVKEN
                     GKLVIRIAIPYRDAKDFAALAGQVLDEQGQPLAGAHVGLMSAPGFFWMPDKLQCATTT
                     DPRGHYRLREIPSRAIDGKPLAVILIVTKEGYAGLQSPRLILTDGDTLKPRVIDPIRL
                     VRGLSLSGVVLDHRGRPVAGALVLSNQRNTPAGPSGVPTSAKTDENGRFTIRGLLRGV
                     ATLSAFEGNVRTNNGYLVDGSTEDVHIKLAERMEVAAARLAPPAPPLAVGQPAPELRV
                     STWSDGRARTLGDLRGKVVVLYFWRIEFPPSVSSLLPLGKLATEFQARGVEFLAIHHS
                     ALDEDVGKEQGRKVLAYKGAPLALAIDQTKDARYALGLTAQEYGLQPGLAPVIIVIDR
                     AGKIAFRSDTAAGAGNLNAVFRQSLQNPANMTEESANDRVEQTLAAEIERTLR"
BASE COUNT          439 a          708 c          717 g          473 t
ORIGIN      
        1 tcttcgtggt ttcctccggt cacgtccaag atcagcggta cacggttggg gctcgcgccc
       61 acgacgccca gccaaaggat cacgtgagcg attcggacct tgtcgtttcg gatgtagagc
      121 aggtcggcgg gcagcaacat ggcgaccagc gcattgtacg gagctcgttc gatggtttcg
      181 acggtcagaa tcccgcgtcc tccgggcccc cgcacctcgc gcctcttggc ctgctggcgg
      241 atgccggtat caagcttcac gcccagggca tagttgtagc aaaacgagct gaagttgctg
      301 caatcgatgc ccttggagtt ccgtccgtaa gcgaccctgt gccacggcca gctcgcggga
      361 gggtcccagt ctgggatgtg gtggtgttga tacggatagc cgatccagcg tgaccctgcg
      421 aggatgacgc gatcctgcat ccaggtcgcc ggccgctggg ccaggtcggg cgcgggagga
      481 tattggcgtg cgcgagggcc ccaggccccc agcatccgtt cgtgccgagg gctgtaccaa
      541 tcgcgcgcgg ggacttccga ttccaggtgg ggattgttcc acggaggcag gtcgaatcca
      601 actccgagat cgggcagcag gtggcgaaat tgcagactgt agggggaccg ataaggcacc
      661 tcctgcgcaa ggcttggacg cccccggttc gacggcgacg ccacgaaccc ggcacacaat
      721 gaacgcagcc agacacgacg attcatcaaa agttgatcct tcgcagaact ccgtccacaa
      781 cggaccgcca tgagtcgata atagctcacg ttttggagct gcgtttccgg ccgatcgcat
      841 gcactttttc caggtgggaa cgtttttctg aagaatctcc ggaggtccgt cgtcgatcgg
      901 gccggagggg taggcacata ttccgtgacg gggtttggat tacggtccga gccggcaggc
      961 gttctttctt tgatcgaccg agtgatgcgt ttgggctctt cagcgcaggg tccgctcgat
     1021 ctccgccgct aaagtttgct cgactcgatc attggccgac tcttcggtca tgttcgctgg
     1081 attctgaagc gactgcctga agactgcatt tagattcccg gcgccggccg cagtgtcgct
     1141 ccggaaggcg attttacccg cccggtcgat cacaataatt accggtgcca ggcctggctg
     1201 aagtccgtat tcctgagccg tcaatcccaa cgcgtatctg gcgtctttcg tttgatcgat
     1261 tgccagggcg agtggtgcgc ccttataggc aagaaccttt cggccctgtt ccttgccgac
     1321 atcctcgtcg agcgccgagt ggtggattgc cagaaactct acgccccgag cctgaaactc
     1381 ggtcgcgagc ttgcccaggg gcagcaatga gctgacgctt ggcgggaact caattctcca
     1441 gaagtaaaga acgacgactt tgcctcgcaa atcgccaagc gtgcgggcgc ggccgtcaga
     1501 ccaagtactt accctcagct ctggggcagg ttgaccgacg gccaagggtg gggcgggggg
     1561 cgcgagccgg gcggcggcca cttccatgcg ctcggcaagc ttgatgtgaa cgtcctcggt
     1621 cgaaccatcg acgagatagc cattgttcgt gcgaacattt ccctcaaacg cggaaagtgt
     1681 ggccactccc cttagcagtc cgcgaatcgt gaatcgcccg ttttcatcag tcttcgcgct
     1741 cgttggtacc ccggaagggc cggcgggagt gttgcgttgg ttcgacagaa ccaaggctcc
     1801 ggccacgggt cttccgcgat gatcgagcac tacgccactt agggaaaggc cgcgaacaag
     1861 ccggatcggg tcgatgaccc gaggcttcaa cgtgtcaccg tcggtcaaaa tcagccgggg
     1921 cgactgcaag cccgcatacc cttcttttgt cacgatcagg ataaccgcca atggcttgcc
     1981 atcgatggct cggctcggga tctcgcgcaa ccgatagtgc cctcgggggt cagtcgtggt
     2041 cgcgcattgc agcttgtccg gcatccaaaa aaacccgggc gccgacatga ggccaacatg
     2101 tgcgccggcg agcggctgtc cctgttcgtc gagcacctgg ccggccaacg ccgcgaagtc
     2161 cttggcatcg cgatagggaa tcgcgatccg gattaccaac ttgccattct ccttgactgg
     2221 ctcgaccgac gccggggcct ggaggatggc cgatttcgca tcggcatcga tcaacctaaa
     2281 gcgacggtct tgtccctcca atagcgagat ctcctccagt tcatcaggcc tgaagcc
//
LOCUS       DATAQL010001249         6048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4706254, whole genome shotgun sequence.
ACCESSION   DATAQL010001249 DATAQL010000000
VERSION     DATAQL010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4706254"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VHS97_21555"
     CDS             complement(<1..1230)
                     /locus_tag="VHS97_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3450855.1"
                     /translation="MARLSSLAQNSTAETAFTVLAIARALKANGKDVIELEIGDSPFP
                     STPSAKRAGIRAIEDNETGYCASLGLPDFRAAAARFVSAEFGYTVTAENVVVASGAKP
                     FEQYFAEAVLDQGDGVLVFSPQFPTYLPNIERRGARGVLAPLKVENGFRPSVEDVKRF
                     VAADPKPRAIILNSPHNPTGGVATREDLAAIAAIVRGTELMVFSDEPYCHMVWSGRHA
                     SILAEPGMLEHTVAAYTFSKSYSMSGWRIGFAVAPPDIVDAIGKLINTSASCSPPFAQ
                     RAAQAALEDDAQTRDDYMERFRRKVDRLCDGLERVEGFRVVRPAGTFYVFPDVRSVCN
                     RLQITSHGLALYLLEGADDQLGVACLGGECFGDAGRGFLRFSCAEPDERIDQAMAFIP
                     KALDRADRVRRYLESNPQ"
     gene            complement(1357..2100)
                     /locus_tag="VHS97_21560"
     CDS             complement(1357..2100)
                     /locus_tag="VHS97_21560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450856.1"
                     /translation="MNRYISDADDFYVNVHLNTEMELPNNRDTVLHFFEQMKKGFPEL
                     RNFYTRENGDLVLEGEKEQESHRWVAIEPRRLCSGQFNPEALDDSFRQHELVLDLAPH
                     LLTISLLDCEALDVMFGFDFNFEGNHDEVVSEALGVGHGLEGLFELRQPQVVNYEPSL
                     TLALNESGQLQCRLAVETRTNALKVRTREFGDDQISVYFTVRQYWGMGPDMSFVESMR
                     QQREIGEEILQQSVIPRIVRPLAQAIASR"
     gene            complement(2368..5601)
                     /locus_tag="VHS97_21565"
     CDS             complement(2368..5601)
                     /locus_tag="VHS97_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450857.1"
                     /translation="MTADATMVPDFYARLGVDPGAERSEIEAALKRLQPAWSMGTRNP
                     KTRHANQLYLDEIPSLRKALLSDPASRAAYDAELAVAQIAEHDRKLDELQKRVRLRAA
                     KGGLSTSDRKLLAVEAAKLGLSEDDVLRVTRPIPDLIESLLSNGDAELDQDPPADVLD
                     PSTQRQIRVALEHLGCRDLYDSLGVSRDAPPSYIALRADEERQRWMKKAQVTAEKTAW
                     LEVIAHAQSHLGSPKARGRYDRTLAQETEYSFQALATFALNGLRHLDASTESALLEEA
                     AALGIPSERADRLIGRFCRQSDVRRENGAVTPLAGSPYSAQSSVAANANGATKYSLLR
                     CCHCAGVTELSPVARKANTARCRHCGSSLKWDCPICKRVAWVDERRCGCGFRQALREP
                     VVRHFEAAQNAFRNFDLEKALEHLERVQALAPNLPGARNGMTKVRQRQVDIERVQLAY
                     ETARAGGRLVSARAAVEAWSRLVDPDSPELQAAKSEVARGLRRAESLAARARNMERTD
                     PAAARDLYRQSLAIAADLADALTGLKRTPPDPPTALDGQVLGDRIRLSWSPPPPDGLG
                     PLTFVIVRKRGGSLNHPGDGTWIAEVSTNEFDDMHAAPGETVGYAILSKRGGVESVTA
                     ISLGPFVFLGDVKDVHVELRDQEVELAWTLPRGVSEVRVIRKRGGPPTGPRDGDRIPS
                     AHDHTVDRNLDNNEIFHYGIYAIYAMADGKLFASPGVVVSARPHPPVSPLEAPRLLLE
                     PGGRVRMDWIEPARGTVKIVRTDRPLLLAAGSRLSQTEADTIAGSWIEPFGPDRAYDP
                     DPPVEGHCYYTPLTGWGNTLTVGHMAAFSRVADPSDLRATRAGGGLGAGSSGTRVTLR
                     WNWASQSSAAMVVARQGTAPVAPNDPSAITVTVHRADYDRQACWSISLPTSLSGPPAE
                     SAFPSIRTAAKSTAEATPIDAGPWHIRVYSISDGDGVRSISPGLEPTAATILPGPNPE
                     VTVSYVLKRPWFPGLPWWVTFRTVPSGKPVPPMVLVGHPRAVPLSVDDGQILTHFPAG
                     RDGAQFPIRATVSLSPHRARVFIDPNVQPDELTPIRLRHPETGTTRV"
BASE COUNT         1180 a         1866 c         1845 g         1157 t
ORIGIN      
        1 ctgcggattc gattccaggt agcgtcgcac ccggtcggcg cgatccagcg cctttggtat
       61 aaacgccatc gcctgatcga tgcgttcgtc aggctcggcg cagctaaacc gcaagaaccc
      121 gcggcctgcg tcaccgaagc actcaccgcc aaggcacgcc acgccgagtt ggtcgtcggc
      181 cccttcgagc aggtacaagg ccaggccatg agacgtgatt tgtaagcggt tacagaccga
      241 tcgcacatcc gggaaaacgt aaaatgtgcc ggccggacga acgacccgga atccttcaac
      301 ccgttcgagg ccatcacaaa gccggtcgac cttgcggcgg aaccgttcca tgtaatcgtc
      361 gcgggtctgg gcgtcgtcct ccaatgccgc ctgggctgct cgctgcgcaa agggagggct
      421 gcacgaagcg gatgtattga tcagcttgcc gatcgcatcc acgatgtcgg gcggcgccac
      481 ggcaaatcca atccgccagc cgctcatgct gtaagacttg ctgaaggtgt aggctgccac
      541 ggtgtgctcg agcatccccg gctccgccaa gatcgacgca tgccggccgc tccagaccat
      601 gtggcaatag ggctcatcgg aaaagaccat taattcggtg ccgcgcacga tcgcggcgat
      661 cgcggctaga tcttcgcgcg tcgccacgcc gccggtcggg ttgtgaggcg agttaagaat
      721 gatcgcgcgg ggtttgggat ccgcggcgac gaaacgtttg acgtcttcca cggagggacg
      781 aaacccattc tcgaccttga gcggcgccag cacgccccgc gcgccgcgac gctctatgtt
      841 gggtaagtac gtgggaaatt gagggctgaa gacaagaacc ccgtcgcctt gatcgagcac
      901 cgcctcggca aagtattgct caaaaggctt tgcgcccgag gcgacaacga cattttccgc
      961 agtgacggtg tacccaaatt cagcggaaac gaaccgggca gccgctgcgc ggaagtcggg
     1021 gagacccaga ctggcgcagt agcccgtctc gttgtcctcg atcgccctga tccccgcacg
     1081 cttggcactt ggagtgctcg gaaacgggct atcgccgatc tcgagctcga tcacgtcttt
     1141 gccgttcgcc ttcaaggccc tcgcgatcgc caacacagta aatgccgttt ccgctgtcga
     1201 gttctgcgca agtgagctta gtcgagccat gatcagtagt gattcgtcgc caggcgccag
     1261 tgaaaagggt tcgatagatt cgcgttaagg atcgatgacc gaacggagag actgttaacg
     1321 cagtggaaga gagcaaccat cattttgcaa ccggcatcag cgcgacgcga tcgcctgcgc
     1381 aaggggacgg acgatgcggg gaatgacgga ctgttggagg atctcttcac cgatttctcg
     1441 ctgctgccgc atcgactcga caaagctcat gtccgggccc attccccagt actgacgcac
     1501 ggtgaaatac acgctgatct ggtcgtcgcc aaactcccgt gtacgcactt tcaacgcatt
     1561 tgtccgtgtc tcgacggcca gccggcattg aagctgaccc gattcgttca gagccaatgt
     1621 cagcgacggc tcatagttga ccacctgggg ctgccgcaac tcgaagagtc cttccagccc
     1681 atgcccgacg ccgagtgctt cgctgacaac ctcgtcgtgg ttgccttcga aattgaagtc
     1741 gaagccgaac atcacatcaa gcgcttcgca atccagcaag ctgatcgtca acaagtgcgg
     1801 agccagatcc aagaccagct cgtgttggcg aaaggagtcg tcgagtgcct cgggattgaa
     1861 ttgtcccgaa caaagccggc gcggctcgat ggccacccag cgatgcgact cctgttcttt
     1921 ctcgccttcc agaacaaggt cgccattctc tctcgtataa aagttccgta actcggggaa
     1981 gcccttcttc atctgctcga aaaagtgcaa caccgtgtcg cggttattgg gaagttccat
     2041 ctcggtgttg aggtgcacgt taacgtaaaa atcatctgca tcagaaatat agcgattcat
     2101 cctgatgatc ctgaatattt cgtcgccaac acctagcccg gcgcgccgcc ccgcctgaat
     2161 gcggtccgtc gaacacggac agggtgcgta ctgatctgtt gtggacccct accatcttaa
     2221 cacaacgcga tttgctccga aatgaatgga cactgggctg cggcaatatc ttgattgtca
     2281 gccgtgccac gaccttgtgt ttaggcaggc gcgtacgacg ggcgaaaaaa gcagatcgga
     2341 agttcgggga cgcggtcggt tctttgctca aacccgcgtc gtgccagtct ctgggtgacg
     2401 gaggcggatt ggggtaagtt cgtccggttg cacgtttggg tcgataaaga ctctagcgcg
     2461 atgaggggaa aggcttactg tcgcacggat ggggaattga gcaccgtcgc gtcctgccgg
     2521 gaaatgggtg aggatctggc catcgtctac cgacagcggc accgcgcgag gatgacccac
     2581 caataccatg ggtggcactg gcttacccga aggcacggtc cgaaacgtca cccaccacgg
     2641 caaacccgga aaccaaggtc tcttaagaac atacgacact gtaacttcag gatttggacc
     2701 cggtagaatg gtggcggccg tcggctccag cccgggtgaa atcgaacgga ccccgtcccc
     2761 gtcgctgatg ctataaactc tgatgtgcca tggtccagcg tctatcggcg ttgcctcggc
     2821 cgtcgacttc gcagccgttc ttatggaagg aaaggcgctc tcggcgggag ggccggaaag
     2881 gctcgtgggt aagcttattg accaacaggc ctgtcggtcg taatcagccc ggtgcacggt
     2941 caccgtgata gccgacggat cgttcggcgc gacaggcgcc gttccctgtc tggcgacgac
     3001 catcgctgcg cttgattggc tggcccagtt ccagcgcaat gtgactcgcg tcccgctgga
     3061 gccggcgccc aaaccacctc cggcccgtgt cgcacggaga tccgacgggt ccgccacacg
     3121 gctgaaagcg gccatgtgcc cgacggtcag cgtatttccc cagccggtca agggagtgta
     3181 atagcagtgc ccctccaccg gcggatcggg atcgtaggcc cgatctggac cgaacggttc
     3241 gatccaactg cctgcgatcg tatccgcctc ggtctgactg agtcggctgc cagcagcaag
     3301 gagcagggga cggtctgtac gaacgatctt gaccgtgcct cgagccggct caatccagtc
     3361 catgcggact cgcccgcctg gttccagcaa caaacgggga gcctcaagcg gcgacaccgg
     3421 cggatgcggc cgtgccgaga cgacgacccc tggcgatgcg aacaatttgc cgtccgccat
     3481 ggcgtaaatg gcgtagattc cgtagtggaa gatctcgttg ttatcgagat ttcggtccac
     3541 cgtatggtcg tgcgcggagg ggatccggtc gccatctcgg gggcccgtgg gcggcccgcc
     3601 gcgcttgcgg atgacacgca cctccgacac gccgcgtggc aatgtccagg ccagctcgac
     3661 ctcctggtcg cggagctcga cgtgaacgtc tttcacatcg cccaaaaaaa cgaacgggcc
     3721 aagcgatatc gcagtgaccg attcgacgcc acctcgcttg ctcaagatgg catagcccac
     3781 agtttcaccg ggcgcggcgt gcatatcgtc aaattcgttg gtgcttacct ctgcgatcca
     3841 ggtaccgtcc ccgggatgat tgagcgaacc cccgcgctta cgaacgatga cgaaggtcag
     3901 cggacccaaa ccatccggag gcggcgggct ccaggatagt cggatgcgat cgccgagcac
     3961 ctggccatcc agggcggtcg gcgggtcagg gggggttcgc ttgagtccgg tcagggcgtc
     4021 ggcgaggtcg gcagcgatcg ccaggctctg gcgatagaga tcgcgcgctg ccgcagggtc
     4081 ggtccgttcc atattccggg cacgcgccgc cagcgactcg gctcgacgca gaccccgagc
     4141 aacctccgat tttgccgcct ggagctcggg tgagtcggga tcgaccagcc ggctccaggc
     4201 ttcgaccgct gcgcgggcgg ataccaggcg tccgccggct cgcgccgtct cgtaggcaag
     4261 ctgaacccgt tcaatgtcga cctgtcgctg gcggaccttg gtcataccgt ttctcgcgcc
     4321 gggcaggttg ggcgcgagcg cctgaactcg ttccaggtgt tccagtgcct tttccagatc
     4381 gaagttgcga aacgcgttct gggcagcctc gaagtgacgc accaccggtt cgcgcagagc
     4441 ctggcgaaag ccgcaaccgc accgccgctc gtcgacccag gccacgcgct tacagatcgg
     4501 gcaatcccat ttgagcgacg atccacaatg ccggcagcgt gcagtatttg ctttgcgagc
     4561 aaccgggctc aactcggtca cgcccgcgca gtgacaacag cgtagcaggc tatactttgt
     4621 ggcaccgttg gcattggccg caacggaaga ttgggccgaa tagggcgagc cggcgagcgg
     4681 tgtgacagcg ccattctcgc gccgcacatc cgactgcctg caaaaccggc caatcaggcg
     4741 atctgcccgc tcggatggga tgccgagcgc cgccgcctct tcaagcaaag ccgattcggt
     4801 gctggcgtcg aggtgccgga gcccgttgag cgcaaaggta gccagagcct ggaatgaata
     4861 ttccgtctcc tgtgccaggg ttcgatcgta ccttccgcgc gccttgggtg agcccagatg
     4921 cgactgtgcg tgcgcaatca cttcgagcca cgcggttttc tccgcggtca cctgggcttt
     4981 cttcatccag cgctggcgtt cctcgtcggc gcggagggcg atataagacg gtggggcatc
     5041 gcgtgatacg ccgagagagt catataagtc gcggcatccg agatgctcga gcgccacgcg
     5101 gatttgccgt tgggtcgagg ggtcgagtac atccgcgggt ggatcctggt cgagctcggc
     5161 gtcgccgttt gagaggagag attcgatcag gtcggggatg ggccgcgtta cccgcaggac
     5221 gtcatcttca cttaaaccga gcttggccgc ctcgacggcc aagagtttgc gatccgaggt
     5281 gctcagccct cccttggccg cgcggagccg aacccgcttt tgcagctcgt cgagcttgcg
     5341 gtcgtgctcg gcgatctggg caaccgccag ctcggcatcg taggcggcgc gagacgcggg
     5401 atcgctcagg agcgccttgc gcagggacgg gatctcgtcg agatagagct gattggcgtg
     5461 ccgggtcttg ggattcctcg tccccatcga ccaggctggc tgcaggcgct tgagcgccgc
     5521 ctcaatctcg gacctctcgg ccccaggatc aacccccagc ctcgcgtaga agtcaggcac
     5581 catcgttgcg tccgctgtca caagcagatt ctccgccctc cgggcctcct gacaaggtgt
     5641 tcgctgattc ttcgtcaggt gccattttcg ggcaacgatc ggccaacgtc cagtcgcgtg
     5701 atgcccatgc gcggcgtaac tcgcccgtgc aagcctggat gccggaaaac gtgtcgccga
     5761 gtgtgcgagg acaggtgtgc agatccacga aaggcctgct tggtcctcac gcttacatgt
     5821 acaggacttc tacatgacgc cggaacaatc gaatcccaaa taatcgcgcc acgtacgttc
     5881 aacgattaca gttataccat ggtgcgtcac tgatcggcac gatcgcctca atcggtcggg
     5941 ttttgcctca cggctcatcg ctcacgaaca gttcctccag ccggagcacg aacccgggca
     6001 gcacggcgcc tccgtcaagc gtctggtctt ccttgagcag aatggatt
//
LOCUS       DATAQL010001250         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2569058, whole genome shotgun sequence.
ACCESSION   DATAQL010001250 DATAQL010000000
VERSION     DATAQL010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569058"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VHS97_21570"
     CDS             <1..395
                     /locus_tag="VHS97_21570"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX3450858.1"
                     /translation="PSRKGILPMTESASTSSLPSGSSESMTRARPLGLIAKYRLDHRN
                     GVNHFLHVGVGWPMVAVAVLILPFRPIWSLTLVAAAYAIMFLGHFVFERNTPTILKQP
                     STPFVIALDVIRNLWSALVRLVNNQRVG"
     gene            483..923
                     /gene="metG"
                     /locus_tag="VHS97_21575"
     CDS             483..923
                     /gene="metG"
                     /locus_tag="VHS97_21575"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEX3450859.1"
                     /translation="MTDPQATRYDALFHKNRESDFACDSTKLTGDLGAKLMADPITYA
                     DFAKLELRVAKVLEARPHPNADRLMLLQIDLGDQQKQIVAGIRHHYDAEKLVGKLIVI
                     VNNLEPAMLRGEPSNGMLLAATSGERVVVLTVDDPECVVGAKIK"
     gene            973..>2219
                     /locus_tag="VHS97_21580"
     CDS             973..>2219
                     /locus_tag="VHS97_21580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3450860.1"
                     /translation="MRRDLGDFQTPPELVAKVLEALGPICPRWPRVLEPSCGRGHFIR
                     GLLDLPDPPGEIQAVEIQESHYEAARALPVGDVARGVRVRIIRADLFELDLKRDLTWH
                     EQGPLLVVGNPPWVTNSELGSLASTARPPRRNIKGMVGLEARTGASNFDVAEAFWLKL
                     VHELADQQPTIALLCKTSVARSVLQFAHRAALPVTAAWVQQIEAARWFGAAVDACLFC
                     VTLGDAQSRPVPSLRVPIFSGLCQRDPDSIMGFAQGHLIADLMAHEQWAFADGVCPMN
                     WRQGLKHDAAGVMELLRDNASGPWRNRAGETVDVERDFVYPLVKGTDLARSPGPAGAR
                     SVLVTQDRLGANTARLAEQAPRLWGYLQSHSAIFSKRKSSIYRGQPPFALFGIGPYSF
                     APYKVAISGLHKVPKFRVLGPAQG"
BASE COUNT          417 a          692 c          645 g          465 t
ORIGIN      
        1 atccctccag gaaaggaatc ctacccatga cggagtctgc ctcgacctcg agcctcccgt
       61 ccggatcttc tgaatcgatg actcgggcgc ggcccttggg cttgatcgcg aaatatcgcc
      121 tggatcaccg caatggcgtc aatcactttc tccacgtggg tgtcggctgg ccgatggtcg
      181 ccgtcgctgt cttgatcttg ccctttcgcc cgatctggtc gctgacgctc gttgcagcgg
      241 cttatgctat catgttcttg ggacacttcg ttttcgaacg caatactccc accatcttga
      301 aacaacccag cacccccttc gtgatcgctc tcgacgtgat ccgcaatctc tggagcgcct
      361 tggtacgatt ggtgaacaac cagcgcgttg ggtaatctga gtttgctccc cgcgctcgaa
      421 gtccagcccg cggagctgct cgggcgcgca gcggaatggc gttcatatat tcacgaactt
      481 ccttgacaga tccgcaggcg actcgctacg atgcgttgtt ccacaagaat cgtgaatctg
      541 atttcgcgtg tgactcgacc aaactgaccg gcgacctggg agcaaaactg atggccgatc
      601 cgatcactta tgccgacttc gccaagctgg agctccgtgt cgccaaggtt cttgaagcgc
      661 gtccgcatcc caacgcggac cgattgatgc tccttcaaat cgacttaggc gatcagcaaa
      721 agcaaatcgt agccggcatt cgacaccatt atgacgccga gaagcttgtc ggcaaattga
      781 ttgtgatcgt caataatctc gagccggcga tgttacgcgg cgaaccatcg aatggaatgt
      841 tgctcgcggc gacgtcgggc gaaagggtgg tcgtgctcac cgtggacgac ccggaatgcg
      901 tcgtgggcgc caagataaaa taggtagcga gcaccgcgcc agcgcaaatg ccggcgggca
      961 ggaaggatcc gcgtgcgacg cgaccttggt gatttccaga cgccgccgga actggtcgcg
     1021 aaagttctcg aagcgttggg cccgatctgc ccgcgttggc ctcgggtgct cgagccctca
     1081 tgcggtcgcg gtcatttcat tcgtggatta ctcgatctgc ccgacccgcc gggcgagatt
     1141 caagccgtcg agatccaaga atcgcattat gaagccgccc gcgccctgcc cgtcggcgat
     1201 gtcgcgcgcg gcgtgcgagt gcgaattatc cgggccgacc tgttcgagct cgacctcaag
     1261 cgcgacttga catggcacga gcagggtccg ctcctggttg tgggcaaccc tccctgggtc
     1321 accaactccg aactcggaag cctggcaagc acggcccgcc ccccccggcg aaatatcaaa
     1381 ggcatggtgg ggctcgaggc ccgcaccggt gcctccaact tcgacgtggc cgaggcattc
     1441 tggctgaaac tcgtacatga actggccgac caacaaccga caatcgctct cttatgcaag
     1501 acttcggtgg cgcggagcgt cctccagttc gctcaccgcg ctgcactgcc ggtcactgct
     1561 gcgtgggtcc agcagatcga ggcggctcgt tggtttggag cggccgtcga cgcatgtctg
     1621 ttttgtgtca cactcggtga tgcgcagtcc aggccggttc catctctacg tgtaccgatc
     1681 ttttcggggc tgtgccagcg cgaccccgac agcatcatgg gcttcgcgca aggacatcta
     1741 attgccgatc tgatggcgca tgaacaatgg gcatttgccg atggtgtttg ccctatgaac
     1801 tggcggcaag ggctcaaaca tgacgccgcg ggggtaatgg agctcttgcg cgacaacgct
     1861 tcgggcccgt ggcgcaaccg cgctggcgaa accgtcgacg tcgagcggga tttcgtctat
     1921 ccgcttgtca agggaactga tctcgcacga tcacccggcc ccgctggcgc gcgctccgtc
     1981 ttagtcactc aggatcgcct gggagccaac accgcacggc tcgccgagca agcgccccgg
     2041 ctgtggggct atcttcagtc gcatagcgcg attttctcga agcgcaaatc atcgatctat
     2101 cgcggccagc ccccgtttgc gctcttcggt attggtccct atagttttgc cccgtacaag
     2161 gtggcgattt ccgggctcca caaagtcccg aaattcagag tactcgggcc tgcccaggg
//
LOCUS       DATAQL010001251         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4096121, whole genome shotgun sequence.
ACCESSION   DATAQL010001251 DATAQL010000000
VERSION     DATAQL010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4096121"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VHS97_21585"
     CDS             complement(<1..912)
                     /locus_tag="VHS97_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450861.1"
                     /translation="MEIAPFRSVLHLRSVSRMVLAVGFGVLGVMMAHHPMILSGFRRI
                     QTDLGDTRLIHYLLEHGYLWAQRDPEHLDFWSPPFFYPVKNVAAYSDLLLSVGPVYWL
                     WRALGASPDQSFGLWMISMSALNYAAGLLLFRKGLGFGAPAAVAGASLVAFGAPRLNQ
                     LNHQQLLPFFYPLLALYALARLAGDLTLGWRARAGYWLLAMGALVAQLYAGVYFGLFT
                     ILGLALAAVVALTMRPCRVVLLEVLWRDAWVIVAAGVAGILLLQPFVSHYRPAALEVR
                     SQYFPLLRALHPGLGSWINVGPRNWFWG"
     gene            complement(917..2044)
                     /locus_tag="VHS97_21590"
     CDS             complement(917..2044)
                     /locus_tag="VHS97_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3450862.1"
                     /translation="MTDDTVARLRREVVEPIKRRFVGRDEIVDLIVLAVTAGEHLFLH
                     GPPGTAKSALIRQFAAAVRGNYFEYLLTRFSEPNEVFGPIDLARLREGTVATVTTRML
                     PEGEFVFLDELFNANSAILNNLLTVLNERVYRRGAEVHKLPLLSLFAASNHLPEDEAL
                     KALFDRFLLRCHVENLKREEMPRLLAAGWELEQAGPLESTVSAADLRALSRQVYRTEL
                     GTIAERYAEVVGKVRDLGVALSDRRAVKVLKLVASSALICGRSAARVSDFWVFRYVWD
                     REEQIAPLAALVAGVIEPHVDEADTHPLAAVPDRVDGDEIARQLDTVATEIDDRSRGL
                     LAVARTREHLAALADRVAWLPDEPARRHLNDRIGDLLKRIG"
     gene            complement(2034..2663)
                     /locus_tag="VHS97_21595"
     CDS             complement(2034..2663)
                     /locus_tag="VHS97_21595"
                     /inference="COORDINATES: protein motif:HMM:NF039424.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450863.1"
                     /translation="MTHTPFHQFLTELLKEGTIVFRSAKAPHDRISQPAVAFLTEAFK
                     IHSAALAGPPIAFDSETAAAAAEFLRQSSWALVNHDERMADLEKRLNMPGPPRTPSQH
                     LSADLTLRYLPQVLRRARGLNPDDPLVERVANLLRCWPLSGVLSDVFEAPIEPVELGS
                     HPGLLLLYAERLIKNDRPGWRPQRSTLTWEYYQLVSEERSMFTGGIHDR"
BASE COUNT          583 a          932 c          880 g          444 t
ORIGIN      
        1 gccccagaac caattccgtg ggccgacatt aatccaggac cctaggccgg ggtggagcgc
       61 acgcaacaag ggaaagtact gtgagcgcac ctcgagagcg gccgggcgat aatgagacac
      121 gaacggttga agcaacaaga tcccggccac gcccgcggca acgattaccc acgcatcgcg
      181 ccagagcacc tcgagcaaaa ctactcggca cggccgcatg gtcagcgcca cgaccgcagc
      241 caatgccaga ccaaggattg tgaacaatcc aaaatacacg cccgcgtaga gctgcgccac
      301 gagcgccccc atcgccagca gccaatagcc cgcccgcgcc cgccagccca gcgtcaaatc
      361 cccggccagc cgcgcgagcg cgtacaaggc cagaagcgga taaaaaaacg gcagcaattg
      421 ctgatgattc agctggttca gtcgcggcgc accgaatgcc accagcgacg cacccgccac
      481 ggccgccggc gccccgaatc cgagtccttt cctgaagagc agcagccccg ccgcatagtt
      541 cagggccgac atcgagatca tccacaaccc gaaagactgg tccggcgacg ctcccagcgc
      601 ccgccacagc cagtacaccg ggcccacgct caggagcagg tccgagtacg cggccacatt
      661 tttcaccggg tagaaaaaag gggggctcca gaaatcgagg tgttccggat cccgctgcgc
      721 ccaaagatac ccgtgctcca gcaagtaatg gataagccgg gtgtccccca ggtcggtctg
      781 gatccggcga aatcccgaga ggatcatcgg gtggtgcgcc atcatcacgc ccagaacgcc
      841 gaaccccacc gcgaggacca tacgcgaaac cgagcgaagg tgcaggaccg aacgaaatgg
      901 ggctatttcc atcctcttat ccgatcctct tgagcaggtc gccgatgcgg tcgttcagat
      961 gccggcgcgc gggctcgtcg ggcagccagg cgacgcggtc ggccaaggcg gcgaggtgct
     1021 cgcgggtccg tgccaccgcc aagaggccgc gcgatcggtc gtcgatttcc gtggcgaccg
     1081 tgtcgagctg tcgcgcgatc tcgtcaccat cgacgcggtc ggggacagcg gccagtgggt
     1141 gagtgtcggc ctcgtcgaca tggggctcga tcacgccggc caccagcgcg gctaaggggg
     1201 cgatctgctc ctcgcggtcc cagacgtagc ggaacaccca gaagtccgag acgcgcgccg
     1261 ccgaccggcc acaaatcagg gccgaactcg ccactaactt cagcaccttg actgccctcc
     1321 gatccgaaag agcgacgccc aagtcgcgca ccttgcccac gacctcggca taccgctcgg
     1381 cgatcgttcc cagctcggtc cggtagacct gcctggagag agcacggagg tcggccgccg
     1441 agaccgtcga ctcgagcggg ccggcttgct ctagctccca gccggccgcc agcagtcgag
     1501 gcatctcttc ccgcttcagg ttctcaacat gacagcgcag caggaaccga tcgaaaaggg
     1561 ccttcaaggc ctcgtcttcg ggcaagtgat ttgatgcggc gaacaacgat aacaagggca
     1621 gcttgtgcac ctcggccccg cggcggtaaa cacgctcgtt gagcacggtc agcaggttgt
     1681 tgaggatcgc gctattggca ttgaagagct cgtccaaaaa gacaaattcg ccctccggca
     1741 gcatccgggt cgttaccgtg gcgacggtcc cctcgcggag ccgagccagg tcgatcgggc
     1801 cgaagacctc gttcggctcc gagaaccggg tcaacagata ttcaaaatag ttgccgcgga
     1861 ccgctgcggc aaattgacgg atcaaggccg acttggcggt ccccggcggc ccgtgcaaga
     1921 acaagtgctc gccggcggtg accgccagca cgatcaggtc gacgatctcg tcgcgcccca
     1981 cgaaccgccg tttgatcggc tcgaccactt cgcggcggag gcgggcgacc gtgtcatcgg
     2041 tcatgaattc ccccggtaaa catagagcgc tcctcactca caagttgata gtactcccat
     2101 gtgagagtcg atcgctgcgg ccgccagcct ggccggtcgt tcttgataag acgctcggcg
     2161 tataaaagca acagccccgg gtggctcccg agttctacag gctctatcgg ggcttcaaaa
     2221 acgtccgata gtactcccga aaggggccag catcgcagca agttggcaac gcgttcgacc
     2281 agagggtcgt cggggttcag gccacgagcg cgcctgagga cctgcggtaa gtagcgtagc
     2341 gtaaggtcgg ccgagaggtg ttgtgacggc gtccgcggcg ggcccggcat gttcaagcgt
     2401 ttctccaggt cggccatgcg ctcgtcgtgg ttgaccaggg cccagcttga ttgccgcaga
     2461 aactcggcgg ccgcggccgc ggtctcggaa tcgaatgcga tgggcggacc cgccagggcg
     2521 gcgctgtgga tcttgaatgc ctcggtgagg aacgccacag ccggctgtga gatccgatcg
     2581 tgcggcgcct tggccgaccg gaacacgatc gtcccctctt tcaatagctc ggtcaggaat
     2641 tggtgaaacg gggtgtgggt cattctggct tcacatccgg ttggctttca tcgccgcgag
     2701 cccggctccc caggtgtggc gatggaagct atttttccag cggttgtgaa gtctaggccg
     2761 tccgcttggg ccaatggcca ccgttgaaac gagcacgatg gctgccagcg ccaaaaacca
     2821 ccacttccgc cagcgcggc
//
LOCUS       DATAQL010001252         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2875309, whole genome shotgun sequence.
ACCESSION   DATAQL010001252 DATAQL010000000
VERSION     DATAQL010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2875309"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VHS97_21600"
     CDS             complement(<1..679)
                     /locus_tag="VHS97_21600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450864.1"
                     /translation="MSCSRPMHDDVTNSLQVYLREIKDESRLTAQDERDLAEAIARGD
                     RDARTRMIQSNLKLVVKIAREFLGRGLALDDLISEGNLGLIRAATEFEPRFGTRFSTY
                     ASYWIKQSIRHALINTTSTIRLPAHMVGLLTKWRRVEQALHRQQGSVPGFDDIALALG
                     LSESQKMLVAQARQALRLTPDGIEAVEAGLLWPVESSKRCEGASTTLHDADERRILRE
                     RLQDLDDR"
     gene            1198..1941
                     /locus_tag="VHS97_21605"
     CDS             1198..1941
                     /locus_tag="VHS97_21605"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3450865.1"
                     /translation="MNTQMTRNVNQTGTITECIRVLEGGETPRRDDAVRELWEYFFAD
                     LMCHARRKLLASNASRGSADEEDAAERAFTKICRSIERGRLKLGNRVDLYKVLRCATT
                     REVINLLHRAKRDSAGSSDEFVLGQIADPAQTPELLWLAYDACVRLLDLLENDELRRI
                     AIWKLSGHTNEAIRIKLACSLATLERILAHIRETWRRRWDEALPNGPAKSGRRQNSLR
                     AGGALTEDRADPLDSGDFTHLLRSLAGLP"
     gene            complement(2035..3036)
                     /locus_tag="VHS97_21610"
     CDS             complement(2035..3036)
                     /locus_tag="VHS97_21610"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3450866.1"
                     /translation="MKQRRMGRTGLKVSEICLGTMTFAGQSDETTSFRILDVATDRGV
                     TFIDTADAYPIPPDPATAGRTEEVIGHWLAAPLARRHQLVIATKCRIRVGDGPNDQGL
                     SRRHILAACEASLRRLRTDYIDLYQAHLPDPETPIEETLRAFDDLVRSGKVRYAGCSN
                     YPAWQLALALGTSERHDWARYDCAQPRYNVLYREIESELLPLCRDQGVGVIVYNPLAG
                     GLLTGKHTGDEPPGPGTRFTLGASGELYRDRYWHRAQFDAVTNLKDYCRRRGWNLATA
                     SVAWVLQQPGITSAIVGASRPEQLDLTLAAPELAFDEDARAAFDAVWWSIPRRPAGR"
     gene            3193..3828
                     /locus_tag="VHS97_21615"
     CDS             3193..3828
                     /locus_tag="VHS97_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450867.1"
                     /translation="MSEQLIDQECVEQTVPETPVVPDWKAMVREEIDQAIVRGDGRWS
                     RALTAVACIHLLAFVICHSLHSPTRGADPRHILVWFIELVAVFVAMRIFAGKGWFWSP
                     QSIHFVGRLWLTLLILSFSLSTLNATIGWETLWFKAAWGTLSSFFFATLAWLITPRFL
                     FLAVQMYVTALLMARFMHWNNLIYGVSWWLALIEVAWVVRMRERECGARTR"
     gene            3984..>4170
                     /locus_tag="VHS97_21620"
     CDS             3984..>4170
                     /locus_tag="VHS97_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450868.1"
                     /translation="MRWLFFDATSPQEAAARAAVVRKIDDWWYHFTCKASDLDDLFAR
                     RSEWDLPKWMDRHLHAVS"
BASE COUNT          800 a         1265 c         1250 g          855 t
ORIGIN      
        1 cgcgatcgtc aaggtcctgc agtcgctcgc gcaggatccg ccgctcgtcg gcgtcatgca
       61 aagtcgtcga ggcgccctcg caccgcttgc tcgactcgac gggccacagc agcccggcct
      121 ccaccgcctc gataccgtcc ggggtgagcc gcagcgcctg gcgtgcttgt gccaccagca
      181 ttttctgact ctcgctcaag ccgagagcca gcgcgatgtc atcaaagccc ggcacgctgc
      241 cctgctggcg atgaagtgcc tgctcgactc gtcgccattt ggtcaaaagt cctaccatgt
      301 gtgccggcag gcggatcgtg ctcgttgtat tgatcaaagc atgacgaatc gactgtttga
      361 tccaatagct cgcgtaggtg ctgaatcgtg ttccaaagcg gggttcaaac tcggtggccg
      421 ccctgatgag ccccaggttt ccttcgctga tcagatcgtc gagcgccagg ccacgcccta
      481 aaaattcgcg cgcaatcttg acgaccagtt tcaggttgga ctgaatcatc cgagtgcggg
      541 catcgcggtc gccgcgagca atcgcctcag ctaggtcccg ctcatcctgc gcggtcaatc
      601 gcgattcgtc tttgatctcg cgcagataga cctgcagaga attcgtcaca tcgtcgtgca
      661 tcggtcgaga acagctcatt ccgggaaagc tccggcggta gcccgcctgc aaaacaagtt
      721 gagagttgag tgaaatcatc caggtcgacc gctctggtct cggccctgag gtcaagtctt
      781 ccacactcca tatcccttca agttgcctaa tcccctcatc gtgggcttaa aacaatcaac
      841 aattccatcg gacggatgaa aaaatgcgac acatcggtcc acttctcctg taataataag
      901 cagatagccg ctcaatggat ccttatggtg aatcccgagg tcaactgcca taaagctgtc
      961 cactttctgc acctgatgga aagccgccaa gcgcctgtgt tccaccgatt gacggacgga
     1021 tcgtccgccc ggagcctttc cacagccgat ttaggtttcg tggcagggtt gcaaagttat
     1081 atattcatag ttaatgaatt tagtagttga gaaatcggac catgagatcg cgtccgaggg
     1141 cggacgcgtc aacaagaaac tcgatcggga cgtcgaccag tcccaatgga ctcgatgatg
     1201 aacacacaaa tgacccgaaa cgtaaatcaa acgggcacca tcacggagtg catccgtgtt
     1261 ttggaagggg gtgagacccc gaggcgagac gatgcggtgc gcgagctctg ggagtacttt
     1321 ttcgcagacc tgatgtgcca cgctcgcagg aaactcctgg cctccaacgc gtcacgagga
     1381 tcggccgacg aggaagacgc cgccgagcgt gctttcacca aaatctgccg aagcatcgag
     1441 cgcgggcggc tcaagctggg taaccgcgtc gacttgtaca aggtgctgcg ttgcgccacc
     1501 acgcgcgagg taatcaactt gctgcatcgc gccaaacgcg attccgccgg gtcaagcgac
     1561 gaattcgtgc tggggcagat cgccgatccg gctcaaacgc ccgaactcct ctggctggct
     1621 tacgacgcct gtgttcggct tctcgacctc cttgaaaacg atgagcttcg tcggatcgcg
     1681 atctggaagc tttctggtca caccaatgaa gcaatccgga tcaagctcgc ctgctcgctg
     1741 gcgacgctcg agcgcatcct ggctcacata cgggaaacat ggagacgaag gtgggatgaa
     1801 gccctgccga acgggccggc caagtccggg cggcgccaga attcgctccg cgccggtggc
     1861 gcgctcacgg aagatcgagc ggatccgctc gattcgggcg atttcacgca tcttctcagg
     1921 agtctcgcgg gactgccgtg atcacgaacg agcagcccgg cggcgatctc cggcgagctt
     1981 tttctcccat tgttcgggcg gatggcagca agtagggcgc aatcgctccg attgctaccg
     2041 tcccgccggt cggcgcggga ttgaccacca caccgcgtcg aaagccgcgc gggcgtcttc
     2101 gtcaaaagcc agctcaggag ccgccagagt aaggtcgagc tgctctggcc ggctggcccc
     2161 gacgatcgcc gaggtgatcc ctggctgttg tagcacccag gccacactcg cggtcgccag
     2221 gttccagccg cggcgccggc agtagtcttt caagttagtg accgcgtcga attgggccct
     2281 gtgccagtag cgatcgcgat agagctcgcc cgaggcgccg agggtgaacc gagtccctgg
     2341 cccggggggc tcgtcgccag tgtgcttgcc ggtcaaaaga cctcccgcca gtggattgta
     2401 gacgatgacg cccacgccct ggtcgcgaca gaggggcaac aactcgcttt cgatctcacg
     2461 gtagagcacg ttgtagcgcg gttgtgcgca gtcgtaccgg gcccagtcgt gcctttcgct
     2521 cgtcccgagc gcgagtgcga gctgccaggc tggatagttc gagcagccgg cgtagcggac
     2581 cttccccgag cgcaccagat cgtcaaaggc gcgcagggtc tcctcgatcg gcgtttccgg
     2641 gtcggggaga tgagcctgat acaaatcgat ataatcggta cgcagccgac ggagactggc
     2701 ctcacaggcg gccagaatgt gtcgcctgga aagcccctgg tcgttgggcc cgtcgccaac
     2761 ccgaatccgg catttcgtgg ctatgaccag ctgatgccgc ctcgccaggg gcgctgccag
     2821 ccagtgaccg atgacctctt cggtccggcc ggccgtcgcc ggatcggggg ggatcggata
     2881 cgcgtcggcc gtgtcgatga aggtcactcc acgatcggtc gcgacgtcga ggatccggaa
     2941 cgacgtcgtc tcgtcgcttt gaccggcgaa ggtcatcgtc cccagacaaa tctccgatac
     3001 cttcaggcca gttcgaccca ttcgccgctg cttcattatc tcctccgcgc ccttgcgcat
     3061 gcttgtcttc cacaaccaag tctaacgcct cgcgtggtag acttcactgc catgcccagc
     3121 gcgagcggcg tcgtcttttt ggtgacgacg gggcgaatcg agattttacc gatacgaacg
     3181 aggttcgccg cgatgagcga gcaattgatc gaccaagaat gcgtggagca gaccgtgccg
     3241 gaaacaccgg ttgtgccgga ttggaaggcc atggtgcgtg aggagatcga tcaagccatc
     3301 gtgcggggcg acggccgctg gtcgcgggcg ttgacggccg ttgcctgcat ccatctcttg
     3361 gccttcgtga tttgccactc gctacacagc ccgacgcggg gcgccgatcc gcgccatatc
     3421 ttagtctggt tcatcgagct ggtcgccgtt tttgtggcga tgcgcatctt cgccggcaaa
     3481 ggctggtttt ggtcaccgca atcgatccat ttcgtgggcc ggctctggtt gacgctcttg
     3541 atcctctcat tcagcctttc gaccctgaat gcgaccatcg gctgggaaac cctctggttc
     3601 aaagcggcct ggggaacact gagcagtttc ttcttcgcca ccctggcctg gctcatcact
     3661 cctcggttcc tgttcctcgc cgttcaaatg tacgtgacgg ccctcctgat ggcccgtttt
     3721 atgcactgga ataacttgat ctacggcgtg tcttggtggc tggccttgat tgaggttgcc
     3781 tgggtggtgc ggatgcggga gcgggaatgc ggggcacgaa cgcgttagcc ggttccggct
     3841 gtgcagatta gccgagaagc aacggaccta gacgtccgat tgttggtagt atccgttgac
     3901 gggacttctg gcggcgaggc tggtcttgac gagaacattc aaggcggtga cgccgaaggc
     3961 gaattgcctt tggagagcac tcgatgcgct ggcttttctt tgacgcgacc agtccgcaag
     4021 aagccgcggc gcgtgccgcc gtcgtgcgca agatagacga ctggtggtac catttcacat
     4081 gcaaggcttc ggacctcgac gatttgttcg cgcgtcgttc cgaatgggac ttacccaaat
     4141 ggatggaccg acatctccac gccgtcagcg
//
LOCUS       DATAQL010001253         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2420204, whole genome shotgun sequence.
ACCESSION   DATAQL010001253 DATAQL010000000
VERSION     DATAQL010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420204"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VHS97_21625"
     CDS             complement(<1..252)
                     /locus_tag="VHS97_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450869.1"
                     /translation="MKSPRQHKFLSGVVLACVGIGLMGESRAQETIKAPTAGAGLSQR
                     PQDEAAIKQVAETFTRAFNAGDANTIASLYTDDAEVIDEY"
     gene            557..1792
                     /locus_tag="VHS97_21630"
     CDS             557..1792
                     /locus_tag="VHS97_21630"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3450870.1"
                     /translation="MTSQQAEAATVRDRDAGSDALLRYRPQFPILERTNYLISNSLGA
                     VPRAVGPSLQSYFDSWATRGVRAWEEGWWTLTADLGDLVAPLIGARAGEVVFQPNVTL
                     AHGVVFSAFDYSVSAPKIVTDAMHFPSILYLIDQERRRGARVAVIPSEDGISVDTQRL
                     IEAIDEQTAIVSLSHILFKSAYVHDIAAITSKAREVEAITVIDGYQAVGTIPIDVRRL
                     GIDVYVGGCLKWLCGGPGAAFVWVDPQVRRRLEPKLTGWMAHQAPFAFAPQLARRDDA
                     WRLLHGTPNISGLYAARPGLEIINEIGIEAIRAKSLLQTQRLLDLSDRAGYRCTTPRD
                     PARRGGTVAIDVDNGYEISKGLKSLDILCDYRPGAGIRLSPHFYNRDDELDAAVAAID
                     EIRSTGAWRAFATNASTVT"
     gene            2214..>2757
                     /locus_tag="VHS97_21635"
     CDS             2214..>2757
                     /locus_tag="VHS97_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450871.1"
                     /translation="MLWRHLCLVRAGLAAGLVGGLTLIGASNTTAQTKTPTAADPESS
                     AGPVAVATETVNLLTASKAGDLDVAARGQGQERVHLSIKNRSARRLNVIVPPGLVAAS
                     TVGQGGAAGGGGRGGMQSMGLGSAANRDGAFGEFQVAGSPEGLRSVGVADETRTRLIT
                     VPVGETIELNIPAVCLNFGWA"
BASE COUNT          512 a          843 c          858 g          544 t
ORIGIN      
        1 atattcgtca atgacctcgg cgtcgtcggt gtagagggat gcaatcgtat tggcgtcacc
       61 ggcgttgaag gcccgcgtga aagtctccgc tacctgcttg atcgctgctt catcctgggg
      121 tcgctgcgag agaccggctc cggctgttgg cgcctttata gtctcttggg ctcggctttc
      181 acccatgagc ccaatgccga cgcaggcgag cacgacgccg gaaagaaatt tgtgttgacg
      241 tggacttttc atggtgccgc acctgttagt ggagagggat cgggatagcg tccaaacgtg
      301 caatcgttgt gccagccatt cacgcggatc agttcaccgt accctcattc acgggtagga
      361 taggatgcat gcccagaagt cattatagga cgtttggtcc cagcatcgag gagcggccgc
      421 tggaattgta cctcggccca tgtgaggtca cggcgatcag cgtgccgcgg aggtgtcaga
      481 ttcggccatc gaacctcccg ggccagttcc gggccgagcg cgtgcttggg gcgaccggcc
      541 tcgtgaggac cataacatga cgagccagca ggccgaggcg gcaacggtgc gcgatcgcga
      601 cgcaggatcc gacgcactcc tgagataccg cccacagttc ccgatcctgg agcggaccaa
      661 ctacctgatc agcaactcgc tgggagcggt gcctagggcg gtgggtccga gtttgcagag
      721 ctatttcgat tcgtgggcga cccggggcgt gcgcgcctgg gaggaaggat ggtggaccct
      781 gaccgccgat ctcggtgacc tggtcgctcc tctgatcggt gcgcgcgcgg gcgaagtcgt
      841 ctttcaaccg aacgtcaccc tggcccatgg ggtcgttttc agtgcgtttg attactcggt
      901 gagtgcgccc aaaatcgtta ctgatgcaat gcactttccg tcgatccttt atttgatcga
      961 tcaggagcgg cggaggggcg ccagagtggc cgtcatcccg tcggaagatg gaatctcggt
     1021 cgacacacaa cgcctgatcg aggccatcga cgagcaaacc gccatcgtga gtctttccca
     1081 catactgttc aaatcggctt acgtgcacga catcgcggca attacctcca aggctcgcga
     1141 agtagaagcc atcaccgtca tcgacggcta tcaggccgtc ggcaccatcc ccatcgacgt
     1201 acggcggctg ggaatcgacg tttacgttgg cggctgcttg aaatggctct gcggtggacc
     1261 aggtgcggca tttgtctggg tcgatccgca ggtccgtcgt cgcctcgagc ctaaactcac
     1321 aggctggatg gcccatcaag cgccctttgc gtttgcgccc caactggcgc gccgcgacga
     1381 tgcctggaga ttgctccatg gcacgcctaa tatctcagga ctgtacgcag cgcggcccgg
     1441 tctggaaatc atcaacgaga tcggcatcga ggcaatccgc gccaagtcgc tcttgcagac
     1501 ccagcgactg ctcgacctgt ccgaccgtgc cggctatcgg tgcacaactc cccgcgaccc
     1561 agccaggcgc ggcggaaccg tcgccatcga cgtggacaac ggctacgaga tctccaaggg
     1621 cctcaagtcg ctcgacattc tctgcgatta ccgcccaggc gcgggcatcc ggctctcgcc
     1681 tcacttctac aaccgagacg acgagctcga cgccgccgtg gccgccatcg acgagatccg
     1741 ttccaccggg gcttggcgcg cgttcgcgac caatgcgtcg acggtgactt gagccggacg
     1801 ctcttttccg ttccgatatt gaccccaaat aatccgatcg tcacttgcaa atcgcctgct
     1861 ccggcaccac gccatgaaca cccggccgga gtcgttaact ctaaaacgag ggtcgacctg
     1921 ccggttcgtt tttgtctttc tggcaagagc ggtgtacact ctgggttgac ggcgctcgct
     1981 tcctttcggc aattcgagca atgccaacga gtctggctgc cgaacaaaaa gaggtggcga
     2041 agtcgaatag cgaagcgttc agccacgtct tccctcactg gggagattgt atcggtggcg
     2101 caactggtag gaatcgaatc atctccatcc gttttggcgg atattcaggg cctgagaggg
     2161 tgcggttagc ccgacccttc ggtgacggcc ccgtccagaa aggtattagc gcgatgctct
     2221 ggcgacacct ttgcctggtc cgggccgggc tggccgcggg ccttgttggt ggcttgacgt
     2281 tgatcggggc gtcgaacacg actgcccaga cgaaaacacc gaccgcggcc gatcccgaat
     2341 cctcagccgg tccggtcgcg gtggcgacgg agacggtcaa cctgctcacg gccagcaaag
     2401 ccggcgatct cgacgtggcc gcacgcggcc agggtcagga gcgggttcat ctctcgatca
     2461 aaaaccgctc cgcccgccgc ctcaatgtga tcgtgccccc gggcttggtc gccgccagca
     2521 cggtcggcca aggcggagca gccggcggag gcggccgcgg cggcatgcaa agcatggggc
     2581 tgggctcggc agccaatcgg gatggtgcct ttggcgaatt ccaggtagcc ggtagtccgg
     2641 aggggttacg gtcggtcggg gtcgcggatg aaactcgcac ccgtctaatc accgtgcccg
     2701 tgggtgaaac aatcgagttg aacatccccg ccgtgtgcct gaattttggt tgggcta
//
LOCUS       DATAQL010001254         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4554573, whole genome shotgun sequence.
ACCESSION   DATAQL010001254 DATAQL010000000
VERSION     DATAQL010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4554573"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VHS97_21640"
     CDS             complement(<1..328)
                     /locus_tag="VHS97_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450872.1"
                     /translation="MMATRLSSSTAARNNRGKNRFCHVVTLVFLAVPVVTPGAWGQVS
                     IPDQGKVVTELEKRGAQVFRDETQPDRPVISVDLDEREFDDAGLSSLKGLTRLQSLSL
                     DGTRITD"
     gene            490..1911
                     /gene="pabB"
                     /locus_tag="VHS97_21645"
     CDS             490..1911
                     /gene="pabB"
                     /locus_tag="VHS97_21645"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX3450873.1"
                     /translation="MSTRVLPSVVHQVLDLPAASLASVIGAWPRSALLESGPGFTEAG
                     RWSILAAYPRLVWEATGSRWSLHADSGVAEYGEGDVLSVLDGLLRRFGLALPGEQPDP
                     HLPPFQGGMIGFLGYDLAPRIERLPRAAARDSRLPDLRLALYDTAVTVDERAGRVELW
                     AWDLTGEGRTAALRRCRAWRKALDRAFRSPKQSSSPPSFLPSITSSFDRETYLATVRR
                     VLEYIAAGDVFQVNLSQRFTAVGRTDPLELYLRLKQVSPAPFAAYLHWQDLAVVSASP
                     ESFFQTRGDTVVTRPIKGTRPRGSSPDEDGRLAADLLASPKDRAELTMIVDLERNDLG
                     RVCEYGSVVVRDPLTIESFAQVHHLVATIEGRVRAGIGPVDVIRAIFPGGSITGAPKI
                     RAMEIIDELEPNRRGLYTGAIGYLSRGGSSGFNIAIRTILVEGDRASFQVGGGIVADS
                     DPEAEYEETLAKGRGMLAALDPG"
     gene            2004..2843
                     /locus_tag="VHS97_21650"
     CDS             2004..2843
                     /locus_tag="VHS97_21650"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX3450874.1"
                     /translation="MIWYRGEIVPDDAPRVSVLDRTFEHGLGLFETLRTWNGQPTLLS
                     LHLERMQRSARELGLTVDPAQLPDAEAVYHLIEASRASLPVGQDVRIRLTLSGGCVTT
                     SASGSTLWMTAGPLPPNREAGGIITQTCEVTADDCLARHKTLNYWRKRIAQTNAAANG
                     SDDVLCLTPDQLVCETCRANVFLIEKRRLYTPDLGGPLLPGIMRAVVLARARHIGLEA
                     VECPLPIDRIRTTDEAFLTSSLRGMLPIARLLHRELPSPGAVTRQLWSAVSCWLEARG
                     KSS"
     gene            2840..3988
                     /locus_tag="VHS97_21655"
     CDS             2840..3988
                     /locus_tag="VHS97_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX3450875.1"
                     /translation="MTTVADLTSWLEQFAPSRLAEPWDNVGLLWGDPGAVVERVMTCL
                     TVTPATAAEAIRERAALIVSHHPILFREAKRIRADLPETAHLWQLARAGIAVASPHTA
                     FDNTRDGINDILCRRLGLAEAVPLRPVASIASGLVATPQSFKVVVFTPLPEREAVSAA
                     AFSAGAGRIGAYQECSFAIPGEGTFFGTGDANPTVGERGRRETVRELRLEFVCPAPLL
                     GTVLASIRAHHSYEEPAIDVYPLHETVANDSPPSGSGRIGRLLKPLGLEEFAMAVGRV
                     LGGVPIAMVGEPGRRVERVALACGAGDEFLKDAARLKADVLLTGEARFHRALEAEAIG
                     IGLITAGHYATERLGVEELAQRIASIFPSVSVWCSQSERDPFRLVGIA"
BASE COUNT          700 a         1301 c         1233 g          810 t
ORIGIN      
        1 cgtcggtgat tctggttccg tcaagcgaca acgactggag acgcgtcagt cccttcaagc
       61 tcgacagccc tgcgtcgtcg aactcccgct cgtccagatc cacgctgatt actggccggt
      121 caggttgcgt ctcgtcccga aagacctggg cgccccgttt ttcaagctcg gtgaccactt
      181 ttccttgatc gggaatgctg acttgtcccc aggcgcctgg cgtgacgacg ggcaccgcca
      241 aaaataccag ggtgacaacg tggcaaaacc ggttcttgcc tcgattgttc cgtgctgccg
      301 tcgagctact caatcgtgtc gccatcatgg ttcctttccg caggagggaa gggcaaagtt
      361 ttaaaaagca atcgtagctt ggttctcgcc ttgggtcgac gtccctacgg ttgcaaggtt
      421 cgcgaggatc gatgaagatc aaaatctgca cacccaggcg gaccatgatt cctcggtttg
      481 aggctcgttg tgtcaacgcg tgttcttccc tcagttgtgc accaggttct cgacctgcca
      541 gccgcctcgt tggcctcggt catcggcgct tggcccaggt ccgcgctgct cgagagcgga
      601 ccaggattta ccgaggcagg ccgatggagc atcctggccg cctatcctcg cctggtttgg
      661 gaggcaaccg ggtcacgctg gtcgttgcac gccgactccg gggtggctga gtacggcgaa
      721 ggagacgtac tatcggttct cgatggcctc ttgcgccggt tcggtttggc acttccaggc
      781 gagcaacccg acccgcacct gccgccattc caggggggta tgatcggctt tcttggctat
      841 gacctcgccc ctcggatcga gaggctgccg cgtgccgccg cgcgcgactc gcgtctgccg
      901 gatctccgcc tggcgctcta cgacacggcc gtcacggtcg acgagcgggc gggtcgggtc
      961 gagctctggg catgggactt gactggcgag gggcgcacag ccgccttgcg tcgctgccga
     1021 gcctggcgca aggccctcga tcgtgccttt cgttcgccta aacaatcatc gagcccgccg
     1081 tcattcctgc cttcaatcac gagctcattc gatcgtgaaa cctacctcgc caccgtgcgt
     1141 cgcgtcttgg agtacatcgc ggccggcgac gtgtttcagg tcaatctgtc gcaacgattc
     1201 acggctgtgg gtcggaccga cccgcttgaa ctctatctgc ggctcaagca ggtcagtcca
     1261 gcgccatttg cggcgtatct ccattggcag gatcttgccg tcgtctcagc cagcccggaa
     1321 tcgtttttcc agacgcgggg cgacacggtg gtgacccggc cgatcaaggg aaccaggccg
     1381 cgcggttcct caccagatga agacgggcgg ctcgcagccg acctgctggc ttcgcccaag
     1441 gaccgggccg aactgaccat gatcgtcgac ctcgagcgca atgacctggg ccgcgtttgc
     1501 gagtatggct cggtcgtcgt ccgcgacccg ctcacgatcg aatcgttcgc ccaagttcac
     1561 cacctggtcg ctacgatcga aggacgggtg cgtgccggca tcggccccgt cgacgtaatc
     1621 cgagccatct tcccgggcgg ctcgatcacc ggcgccccca agatccgcgc gatggagatc
     1681 atcgatgagc tcgagcccaa ccgccgcggt ctctacaccg gcgcaatcgg ctacctgagc
     1741 cggggcggat cgagcggctt caacatcgcc atccgcacga ttctggtcga gggggaccga
     1801 gccagcttcc aggtcggcgg cggcatcgtc gccgattccg acccggaggc tgaatacgaa
     1861 gaaacgctgg ccaagggccg aggtatgctc gccgctctcg atccagggta ggaggtctag
     1921 gggcaatacc gttcaattag atgtaacttg aacctgtaag ttactgctaa ttcaacgata
     1981 ttaggtctag ggggattcat ccattgatct ggtaccgtgg tgaaatcgtc cccgatgatg
     2041 cccctcgcgt cagcgtgctc gaccgcacct tcgagcatgg gctggggctc ttcgagacgt
     2101 tgcgcacgtg gaacggccag cccacactgt tgtcccttca cctcgagcgg atgcagcgct
     2161 cggctcgcga gcttggattg actgtcgacc cagcccaatt gcccgatgcc gaagccgtct
     2221 accatctgat cgaggccagc cgtgcctcgt tacctgtcgg acaagatgtc cggatccggc
     2281 tcacactgtc cggcggttgc gtcacgacct ccgcctctgg ctcgaccctc tggatgactg
     2341 cgggtccgct gccacccaat cgtgaagccg gcgggatcat tacccagacc tgcgaggtga
     2401 ctgccgatga ctgcctcgcc cgccataaga cgctcaacta ctggcgcaag cgaattgctc
     2461 agaccaatgc ggccgcgaac ggatccgatg atgtgttgtg cctgacaccc gatcagctcg
     2521 tctgcgaaac ctgccgggcc aacgtcttcc tgatcgaaaa gcggcgcttg tacaccccgg
     2581 acttgggtgg tccgctgctt cccggaatca tgcgcgcagt cgtgctggca agggcgaggc
     2641 atatcggtct ggaagccgtc gaatgtccgt tgcccatcga tcgaatccgc acgaccgacg
     2701 aagcgtttct gaccagctcg ttacgaggta tgctacccat agcgcgactg ctccatcgcg
     2761 aattaccaag cccaggagcg gtgacgcgcc aactttggag cgccgtctct tgctggctcg
     2821 aagcccgggg taagtcttca tgacgaccgt tgccgatctt accagttggc tggagcaatt
     2881 cgccccgtcg cgcctggccg agccatggga caacgtggga ttactctggg gcgatccggg
     2941 ggcggtggtc gagcgtgtca tgacctgcct gacggtcact ccggccacgg ccgccgaggc
     3001 cattcgcgaa cgagccgcgc tcatcgtcag ccatcacccg atcctcttcc gcgaggccaa
     3061 aaggatccgc gccgacctgc cggaaaccgc ccatttatgg cagctcgccc gcgccgggat
     3121 cgcagtggcc agtcctcata ctgcgtttga caatacgcgc gacgggatca acgacatcct
     3181 ttgtcggcgg ctgggcttgg cggaggcggt gccgctccgg ccggtagcgt cgatcgcgag
     3241 cggcttggtg gccacgccgc agtcgttcaa ggtcgtcgtc ttcacccctc taccggagcg
     3301 cgaggccgta tcggcggcgg cattttcggc cggtgccgga cgcatcggcg cgtatcaaga
     3361 atgctcgttc gccatcccgg gcgagggtac gtttttcggc accggcgacg ccaatccgac
     3421 ggtgggagag cgcgggcgcc gagagacggt ccgcgagctc agactggaat ttgtgtgtcc
     3481 ggcgccactg cttggcacgg tactcgcatc aatccgtgcc catcattcgt acgaagagcc
     3541 cgcaatcgac gtttacccgc ttcacgaaac cgtcgccaac gattctccac cgagcgggtc
     3601 aggacggatc ggccgtttgc taaaaccctt gggcctggaa gagtttgcca tggccgtcgg
     3661 tcgggtgctt ggcggcgttc ccatagcgat ggtcggcgaa cccggccgcc gggtcgagcg
     3721 cgtagccctg gcatgcggcg cgggtgatga attcctcaag gacgccgccc ggctcaaggc
     3781 tgacgtatta ctgacgggcg aggctcgttt tcaccgcgcg ctcgaagccg aagccattgg
     3841 catcggcctg atcacggcgg gtcattacgc caccgaaaga ctgggcgtcg aagaactcgc
     3901 ccaacggatc gcctcgatct tccccagcgt tagcgtctgg tgcagccaaa gcgagcgcga
     3961 cccgttccga ttggtcggaa tcgcctgatt tgcaagtcgc gttatttttt cttgctcttc
     4021 gaggatgaag cttgagctcc attc
//
LOCUS       DATAQL010001255         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1808312, whole genome shotgun sequence.
ACCESSION   DATAQL010001255 DATAQL010000000
VERSION     DATAQL010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808312"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VHS97_21660"
     CDS             complement(<1..1318)
                     /locus_tag="VHS97_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3450876.1"
                     /translation="MRSLWRWHRSVRCWLIGLAISVSGLTAHAADGKRPPNVVLIVAD
                     DLGWSDVGFNGRTEWTTPNLDRLAKLGRVLDRCYTAAVVCAPSRAAFLTGKYTIHSGV
                     RRNDDDLPTEEVTIAEAIKPLGYRAALFGKWHHGKSRADSEQYVHPLDQGFDEFFGYT
                     DAVHAWEKFPTKLWNGREQIPASGYIDDLITDRAIQFVGEQRQKPFFLYLAYVATHFG
                     IAAPADEVARHEGRFSEENPDLPLKATYAAMVTRLDRNIGRLLKTLEQLDLTRDTLII
                     FTSDHGATFESGNQGTSASLDSNRPFRGQKRTLWEGGVRVPALAYWPGKIAGGVRTRD
                     VTHLTDLLPTIVAAAGGKVDPAWHIDGANMLPVWTGKQPAPERTLFWEWRSEGYDQLA
                     ALRGQYKLVVTHGGKAELFDMEDDPAERRDVSAHHPDLTKQLKAELL"
BASE COUNT          326 a          519 c          451 g          352 t
ORIGIN      
        1 cgaggagctc ggctttcaat tgcttcgtca gatccggatg atgcgcggaa acgtcgcggc
       61 gctccgcggg atcatcctcc atgtcgaaca attcggcttt tcccccgtgc gtgaccacga
      121 gcttgtattg gcccctgagt gcggccagtt ggtcgtaacc ctcactccgc cattcccaga
      181 acaacgtgcg ctcgggcgca ggctgtttcc cggtccagac cggaagcata ttggctccgt
      241 cgatatgcca ggcgggatcg accttgcctc ccgcggccgc cacgatcgtg ggcagcaggt
      301 cggtcagatg ggtcacgtcg cgcgtcctga cgccacctgc aatcttcccc ggccagtaag
      361 ccaaggccgg cactcgaacg cccccttccc agagcgtccg cttctggcct cggaatggcc
      421 gattactgtc tagcgatgca cttgttccct ggttgccgct ctcgaacgta gcgccatgat
      481 cgctggtaaa aatgatcagc gtgtcacgcg tcaaatccag ttgttcgagt gtctttaaga
      541 gccggccaat gttacggtcg agccgagtga ccatcgccgc gtatgtcgcc ttcaagggca
      601 ggtcgggatt ttcctcggaa aatcggcctt catgccgcgc cacctcatcc gcgggggcgg
      661 cgatcccgaa atgggtggcc acataagcga ggtacagaaa gaacggcttc tgcctctgtt
      721 cgcccacgaa ttggatggct cggtccgtga tcagatcatc aatgtagccg gatgccggaa
      781 tctgctctcg gccattccaa agcttggtcg gaaatttctc ccaggcatgt acagcgtcgg
      841 tgtaaccgaa gaactcgtcg aatccttggt ccaacgggtg aacgtactgc tcgctgtccg
      901 cgcgcgattt gccgtggtgc cacttgccga aaagagccgc cctataccca agcggcttga
      961 tcgcctcggc gatcgtcact tcctcggtcg gcagatcgtc gtcgttgcga cgtacgcccg
     1021 agtgaatcgt gtatttcccc gtcaaaaagg ccgcccgact gggggcgcac accacagcgg
     1081 ccgtgtagca gcgatcgagc acccggccca gtttcgccag cctatccagg ttgggcgtcg
     1141 tccattctgt gcggccgtta aagccgacat cgctccagcc aaggtcgtcc gcgacgatca
     1201 aaacgacgtt cggcggccgc ttgccgtcgg ctgcatgcgc ggttaatccg gaaactgata
     1261 tcgccagccc aataagccag cacctgaccg acctgtgcca tcgccagagc gatctcatca
     1321 ccatgcccca acggaaaacc actcgaggtc gcaagaaacg tgaaggtgac agacgtgcgt
     1381 caggcatcca gtcgccgtac ctcacacctt ctggccgccg acgacctccg tgtccgcttg
     1441 gattcactat cggtcttaaa acacagcgct gtccaactaa tctcatctaa atcgaccgaa
     1501 taagtcaaga actactatcc caaattggtc cagatgtccg gaagaatctt cacgtccccc
     1561 gatgtgccgt cgattattat cgttctccct atggtggagt gattgctggt ttgcgggccg
     1621 gtagattggt ttactgacaa tcttgcga
//
LOCUS       DATAQL010001256         4584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4554698, whole genome shotgun sequence.
ACCESSION   DATAQL010001256 DATAQL010000000
VERSION     DATAQL010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4554698"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="VHS97_21665"
     CDS             <1..1986
                     /locus_tag="VHS97_21665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450877.1"
                     /translation="LDARCDLYSVGVILFELLTGKQPFETPTGAVREMLLRMIADRRG
                     PVPKLRAWNAQISPAVESIVRHCLEPDPSHRYRSARELQEDLERQLDNLPLKHVREPS
                     VRERIGKWIRRNRRLASVTTVVGVATCLLAFVTAGFLVRQRHLGRLEASDSLHRLSHE
                     LQQVNLMLGSRDASRQEINEGIDLCRQVLGRYHVLDDPSWRDRPIVRHLQEDERGRLR
                     QEVGQLLLLYARAITWQVETTTDLERRSERHRFASLLNALAESCFGETAPTRALWRQR
                     AELARLSGNGDESQQLRVRAEAIPLRTPMDRFWDVLDRLDRTEPEDLETEVGKRRTLM
                     AEVQQISRVDPQNFVNWLLLGNCYVRLKQLHEAKNCYTTAIALEPELSWSYVNRGLVH
                     LDLNEFSDALLDFDRVIALRPDMIEAYINRALARMGLANFTGAVRDLSRVLDNPDAPV
                     RAFFLRARARERLGDREGAANDRALGLARQPSDELSWVVRGLAQLPANPRGALSDFDA
                     ALALNPRSRSALQNKAHVLSEQLGRPEDAIRALDTALQYYPDSGEALGGRGVLLARLG
                     RRKVALADARAALAVDNEVLTIYQVAGIYALTSKEEADDRREALRLLAIALRRDPAWL
                     KVIPVDHDLDPIRDHPEFRELLRAVAVVSGAGVAGKG"
     gene            2091..4046
                     /locus_tag="VHS97_21670"
     CDS             2091..4046
                     /locus_tag="VHS97_21670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX3450878.1"
                     /translation="MPATFGVPWTDASRLTLSFAPDGTSIAGQSSSLFQTLNAEQATS
                     AWQQEILEAFQTWAVHANINIGLVSDGGQPFGVSGLSQHDPRFGDIRVGAQPMASDAM
                     SISVPNDPALTSSWTGDVLLNSNDSFGGNGADLYSVLLHEAGHVFGIGDSTDPNSPMY
                     SQYLDNTQLTANDISAFQSLYGVRTLDPHEGSSGNDSMSKATQIQFPGAYTGTTPLVV
                     YGDIGSNSDSDFYSVKPPSNYNGPVTFQLQSAGISLLTPKLTVLDSNGNVLGQAQAAS
                     DFGGTVTVHLNQSSSNATYYLEVQGATQDVFGIGSYGLAVTYDATNTVTAAALQSVLV
                     GPYQNLGSNDINAIFLNPMGCLFNNQNGSGGGGGGSGGGGSTTQLASTPGYAVNTHYE
                     TMASLASPSEVDTYGIAAPSTSNGQHLVLTASVRAVAPNGVAPIVTILDGSQNVVAAQ
                     ILSNGNGVYTIQAANLMSGGNYTLHVSTEDSTTGPGNFDLDAVFGKTAADLSTFASGS
                     LAAAAAQQSYNYYVGESQLFQFLLSANSQGAPAGSSVQMTIADSSGNIVYTLTAQAGE
                     TVSNAALFLTPGAYTLSFSVLVPAGSPVPQLSYNLAGDSFSDPIGPVVLDPTQTPVYT
                     SPTTPGTFLYPNGTATKSAFLIVPIVS"
     gene            complement(4308..>4584)
                     /locus_tag="VHS97_21675"
     CDS             complement(4308..>4584)
                     /locus_tag="VHS97_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450879.1"
                     /translation="RAGNRQQHQEPDQLDTPEHGFISTGPAKSPNRVLQQNCRITFVP
                     LIWAIVTRVDPSWDDRGGTTANTARHHAIVAFAEFLAFFFGWILEKL"
BASE COUNT          902 a         1450 c         1347 g          885 t
ORIGIN      
        1 ctcgatgcac gctgcgacct ctattctgtc ggcgtgattc tcttcgagtt gctcacgggc
       61 aagcagcctt tcgagacgcc cacaggcgct gtccgcgaga tgctactcag aatgatcgct
      121 gatcgccgcg ggccggttcc caagttgcgg gcatggaacg cccaaatttc gcccgccgtc
      181 gaatcgattg tccggcactg cctcgaaccg gatccctccc accgatatcg atctgcgcgc
      241 gaactccagg aagaccttga gcggcaactc gacaatctgc ctttgaaaca tgtccgcgaa
      301 ccgtcggttc gcgagcgaat tggtaagtgg attcgccgga accgacggct cgcttcagtg
      361 acgaccgtcg tgggcgtcgc gacttgtctc cttgcgtttg tgaccgccgg attcctcgtc
      421 cgccagcgtc acctcggcag gctggaagca tctgattcgt tacaccggct gtcgcacgag
      481 ttgcagcagg tcaatctgat gcttggatcg cgcgatgcca gccggcaaga gatcaacgag
      541 ggaatcgacc tctgtcgaca agtgctgggc cgataccacg tcctcgacga cccatcttgg
      601 cgtgatcgcc cgatcgtgcg acatctgcaa gaggacgagc gcggccgact ccgccaggaa
      661 gtcgggcaac tgctgcttct ctacgcgcgg gccataacct ggcaagtcga gacaaccacc
      721 gacctcgagc ggcggtccga gcgtcatcgg ttcgcctcac tattgaacgc gctcgccgag
      781 tcgtgctttg gggaaacggc cccgacccgt gccctctggc ggcagcgcgc cgagctggcg
      841 cgtctcagcg ggaacggcga tgagtcccag cagcttcgcg tgagggccga agccatccct
      901 ctccgtacgc caatggatcg gttctgggat gtgctcgacc ggcttgaccg aaccgaaccc
      961 gaggatctcg agaccgaggt cgggaaacgc aggaccctca tggccgaagt gcagcagatc
     1021 tctcgagtcg atccgcagaa cttcgtgaac tggttgctcc tgggcaactg ttatgtccgg
     1081 ttgaagcagc ttcacgaggc caagaactgc tacacaacgg ccatcgcgct cgaacctgag
     1141 ctctcctggt cctacgtcaa ccggggattg gtccacctcg acctcaacga gttctcggac
     1201 gcgcttctcg atttcgaccg cgtgatcgct cttcggcctg acatgatcga ggcgtacatc
     1261 aaccgtgccc tcgcacgaat ggggctggcg aacttcacag gcgccgttag ggacctcagc
     1321 cgtgtgcttg acaatccaga tgccccggtc cgcgccttct ttctacgggc cagagcgcga
     1381 gagcgtttgg gcgaccggga aggcgccgca aacgaccggg ctctgggcct ggctcgccag
     1441 ccgagcgacg agctgagctg ggtcgtgcgg ggcctcgcac aacttcccgc taatcctcgc
     1501 ggcgccctgt ccgactttga cgccgcgctg gcactaaatc cgcgatcgcg tagcgcgctg
     1561 cagaacaagg cccatgttct ctccgagcag cttggacggc cggaggacgc catccgcgcg
     1621 ctcgacaccg cccttcaata ttaccccgac agtggggagg ccctcggcgg gcgcggggtg
     1681 ctcctcgcga gacttgggcg ccgcaaagtg gcgctcgcgg atgctcgggc ggccctcgcc
     1741 gtcgacaacg aagtcttaac gatctaccag gtcgccggga tttacgcgtt gacatctaaa
     1801 gaagaggccg acgaccgccg tgaagccctc cggcttctgg ccatcgccct gcgcagagat
     1861 ccggcgtggc taaaagtcat tcctgtcgat cacgacctcg acccgattcg cgaccaccct
     1921 gaattccggg aactacttcg ggccgtagcg gtggtctccg gcgccggggt cgccgggaag
     1981 ggctaaggag cgaaactgat attttcagct ttgggggaaa cgccatgttg tgcaagagaa
     2041 agaggaacag gcggcaaaat ctcgtggtcg agcgcttgga aggccgcgcc gtgcctgcga
     2101 cctttggcgt cccctggacc gatgccagcc gcctgacgct aagcttcgcc ccggacggta
     2161 cctcgattgc tggccagagc agctcgctgt tccagactct caacgcagag caggcgacgt
     2221 ctgcctggca gcaggagatc ctcgaggcgt tccagacgtg ggccgtgcac gcgaatatca
     2281 atatcggcct tgtgagcgac ggcggtcagc catttggcgt gtcgggcctg tcgcagcacg
     2341 accctcggtt cggcgacatc cgagtcggcg cccagccgat ggcgtccgac gcaatgtcga
     2401 tatcggtgcc gaatgatccc gcgctgacaa gctcttggac gggcgacgtc ttgcttaact
     2461 ccaacgacag tttcggcggg aatggggcgg acctctactc ggtattgctg cacgaggcag
     2521 ggcacgtttt cggcataggc gacagcaccg atcccaactc gccaatgtac tctcagtact
     2581 tggacaacac gcagcttaca gccaacgaca taagcgcctt ccagagccta tacggcgtgc
     2641 gtacgctgga tccgcacgaa ggatcctcgg gcaacgattc gatgtccaag gccacgcaga
     2701 tccagttccc cggcgcttac actggaacta cgcctttggt cgtttacggc gatatcggct
     2761 cgaattccga ctccgacttt tactccgtta agcccccgag caactacaat gggcccgtga
     2821 cgtttcaact tcaaagcgcg gggatcagcc tgctgacgcc aaagttgacc gtcctggact
     2881 ccaacggcaa tgtcctcgga caggcgcagg ctgcgagcga tttcggcggt actgtcaccg
     2941 tacacctcaa ccaatcgagc tccaacgcga cgtattatct cgaagtccag ggcgcgaccc
     3001 aagacgtctt cggaatcggc agctacggcc tggcggtcac ttatgacgcg accaacacgg
     3061 tcacggccgc ggctctgcaa tcggttttgg tcgggccgta tcagaacctc ggttccaacg
     3121 acatcaacgc gatctttctg aacccgatgg gttgcctctt caacaaccag aacggaagcg
     3181 gtggcggcgg cggcgggagc ggcggtggcg gatcgacaac ccagctcgcc tcgactccgg
     3241 gctacgcggt caacacgcac tacgagacga tggccagcct cgcaagcccg tctgaggtgg
     3301 acacctacgg catagccgcc ccgagcacat cgaacgggca acacctcgtg ctcactgcca
     3361 gcgtccgggc cgtcgccccc aacggcgtcg ccccgatcgt tacaatcctc gatggcagcc
     3421 agaatgtggt tgccgcgcaa atcctctcta acggcaacgg cgtctacacg atccaggccg
     3481 ccaatctgat gtcgggcggc aactacaccc ttcacgtctc caccgaagat tcgacgacgg
     3541 ggcccgggaa ttttgacctg gacgccgtgt tcggtaaaac ggccgccgac ctttcgacgt
     3601 tcgcgagtgg tagtctcgcg gcagcggcgg cccagcagtc ttataactac tacgtcggcg
     3661 aaagccagct ctttcagttt ttgctctcgg cgaacagcca aggcgcacct gccggatcgt
     3721 ctgtgcagat gacgatagcc gattcgagcg gcaacattgt gtataccctc acggcccaag
     3781 caggcgaaac cgtcagtaac gcggccctgt tccttacgcc cggagcgtac acgctcagct
     3841 tctcggtgct ggtgccggcc ggtagccccg ttccgcagct ctcgtacaac ttagcggggg
     3901 attcgttcag cgacccgatc ggccctgttg tattggaccc gacccagact cccgtttaca
     3961 cctcgccaac aacgccgggg acgttcctgt atcccaacgg caccgccacc aaaagcgcat
     4021 tcttgatcgt gccgatcgtg agctgaaaga ccagatcgac ttgagttcag aagactgatc
     4081 gcagcatgcc gaaatcatgg taaattctcc ctaagggaag cagataaccg ggcgtgctcg
     4141 aatccgtaga tttgggcatc gttcggtcgt gatttcgacg gttgacactg atgagatctt
     4201 gattcgacga gcattagcgg gttgtcgtct ggctcctcgg gagcccaaac accctctgcc
     4261 tctgtgcagt tcttgcgcca taccatagcc cgctggccaa tcgacggtta cagtttttcg
     4321 agaatccatc caaaaaaaaa tgcgaggaac tctgcaaagg cgacaatagc gtgatggcga
     4381 gcagtatttg cggtcgtacc gcctcggtcg tcccaggagg gatcaacacg cgtgacaatt
     4441 gcccagatta atgggacaaa ggtgattcga cagttttgtt gcagaacgcg attgggactt
     4501 ttcgcgggcc cagtggagat gaagccatgt tctggcgtgt cgagttggtc gggctcctga
     4561 tgttgttggc gattgccggc tcta
//
LOCUS       DATAQL010001257         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3029612, whole genome shotgun sequence.
ACCESSION   DATAQL010001257 DATAQL010000000
VERSION     DATAQL010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3029612"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VHS97_21680"
     CDS             complement(<1..242)
                     /locus_tag="VHS97_21680"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX3450880.1"
                     /translation="MGDLRADVLRGLRGALVGRPGMDDAFFEDNVQETLLKVLDRLET
                     FAGRGRFVSWALAIAIRVAQTTIRKQRWQGVSLDRF"
     gene            complement(577..1050)
                     /locus_tag="VHS97_21685"
     CDS             complement(577..1050)
                     /locus_tag="VHS97_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450881.1"
                     /translation="MSGLQISRTNTESARNGSFRRQASALAQLVIDEQRREAVHSVAS
                     IMGSLREHRLDLLRHRLSSHRLAAKPGRPRRDTLLDRDEAAREEGRAVAEYEQTLAEL
                     ESLGIRCLDSAAGLAIFTVYQDGSLREYVFDLFDSQPLRFPDEHVPGRGQQRRRE"
     gene            complement(1074..2120)
                     /locus_tag="VHS97_21690"
     CDS             complement(1074..2120)
                     /locus_tag="VHS97_21690"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX3450882.1"
                     /translation="MRDRALRALALFLILTVLCPSGAGAGEKPVDAPKKVLVELYTSQ
                     GCSSCPPASDLLGKLFELGDGPDRIVPLNFHVDYFNQPWPDPYSDASYTRRQRDYNTV
                     QRRDDLQFTPLLMVDGRQPLIGSDRAKAVAVIEQALKQPPEVALDLDLDGTGIRKTVS
                     VKIAARSREVAGRELLIGVALTEDPVTTKVLRGENAGLTLVEHHVVRRLDHQFLKLDR
                     TGSRTLTFPIELPSGRAGTRFRVTVFAQDRANGNVYQANEVPWNPQNATSFRADRARD
                     LDRVVARRKARRAALNRLISPPGFDFRAMANDDSDIVVLTICPACGSLFNSRTIASNY
                     RRPGQGFPPASATC"
BASE COUNT          382 a          694 c          768 g          425 t
ORIGIN      
        1 ttgaaccggt ccagcgacac gccctgccac cgctgtttcc gtatcgtggt ctgcgccacg
       61 cggatggcga tggccagggc ccaactcacg aaccgacccc gccctgcgaa cgtttccagg
      121 cggtccagca ctttcaggag cgtctcttgc acattgtcct cgaagaacgc atcgtccatg
      181 cccgggcgcc ccaccagcgc gccccgcaag ccgcggagca cgtcggcgcg caagtctccc
      241 aacgcggcct cccgacgggg gccggattgc cttaggtcct caatccaggc gtcattcgcg
      301 cgatccattt cgagagtccg ctggcaacgc cgggccgcct gtcaaccgca ggcgtcgggc
      361 agtgccgccg ggaagtcgag caatgtacgg gcttcgtgga agtcggtcaa cgggtccggg
      421 gcgacgcagg cgaccctctc cgcggttctg ctctaagtct agcctgactg ccgcacctgg
      481 gccacctccc ctcggctcat ctcaagaccg gtttcgagtt gcgacctgcg tcatttttga
      541 cgttggtctg ttgcttcctg ggtcgccatg ctccggctat tctcgtcgac gctgctggcc
      601 cctgccgggc acgtgctcgt ccgggaagcg taatggctgc gagtcgaaca ggtcgaagac
      661 atactccctg agggatccgt cctggtagac ggtgaagatg gccaggccgg cggcggagtc
      721 gaggcagcgg atgccgagcg actcaagctc ggcgagggtc tgctcgtatt ccgcaacggc
      781 tcggccttcc tcgcgggcag cctcatcccg gtcgagcagt gtgtcccgcc tgggcctgcc
      841 gggcttggcg gccaatcgat gggaggagag gcggtggcgc agtaagtcta acctgtgctc
      901 gcggagcgag cccatgattg aggcgactga atggacagcc tcgcgccttt gctcatcaat
      961 gacgagttgt gcgagggcgc tcgcctgtcg gcgaaaggag ccgtttctgg ctgattccgt
     1021 gttggtccta ctgatttgga gcccggacac tccgggcgga ctcagtcgcc cattcagcag
     1081 gtcgcgctgg ccggagggaa cccctgacca ggacggcgat agttcgaagc gattgtccgt
     1141 gaattgaaga gggagccgca ggcgggacaa attgtgagga cgacgatgtc ggagtcgtcg
     1201 ttcgccatag cccggaaatc gaatcctggc gggctgatga gccggtttaa cgcggcgcgg
     1261 cgtgccttgc gtcgtgcgac gactcggtcg aggtcgcgag cacgatcggc gcgaaaactt
     1321 gtggcatttt gtggattcca gggcacttcg ttggcctggt agacgttgcc gttggcgcga
     1381 tcctgggcga agactgtcac tcgaaagcgc gttccggccc ggccgctggg gagttcgatg
     1441 gggaaggtga gggtccgaga tcccgtccga tcgagcttga gaaactggtg gtcgagtcgg
     1501 cggacgacgt gatgttcgac gagcgtcagt cccgcgtttt cgccgcggag aaccttggtc
     1561 gtgaccgggt cttcggtcag ggccaccccg atcagcaact cgcgtccggc cacctcgcga
     1621 gatcgggcgg cgattttcac ggagacggtc tttcgaatcc ccgtgccgtc caagtcgagg
     1681 tctagggcga cctccggcgg ctgcttgagg gcctgttcga tgaccgccac ggctttcgcg
     1741 cggtccgacc cgatgagcgg ctggcggccg tcgaccatca agagcggggt aaactgcagg
     1801 tcgtcgcggc gctgcacggt gttgtaatcg cgttggcggc gggtgtacga agcgtccgaa
     1861 taggggtcgg gccacggttg gttgaaatag tcgacatgga agttgagtgg cacgatccgg
     1921 tcggggccat cgcccagctc gaatagcttg ccaaggaggt ccgaagcggg tgggcaggaa
     1981 ctgcacccct gcgaggtgta gagctccacg agcacttttt tcggcgcgtc caccggcttt
     2041 tctccggccc ccgccccgga aggacagagc acggtcaaga tgagaaatag ggcgagtgcc
     2101 ctcaacgcac ggtctctcat gagagtcgcc tcgatcgagt cgcgcgcccc ggatcggaag
     2161 gattgacacg ccgtcgggcc tggtccggcg aaatcgcctc tcaggatctg tcgtagggcg
     2221 gaaaaaccct gacaacccct ggttcaaact cggccgcgcg cggaaatgg
//
LOCUS       DATAQL010001258         7964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3029690, whole genome shotgun sequence.
ACCESSION   DATAQL010001258 DATAQL010000000
VERSION     DATAQL010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3029690"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2668
                     /locus_tag="VHS97_21695"
     CDS             <1..2668
                     /locus_tag="VHS97_21695"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450883.1"
                     /translation="SLAALGEPGRAEIVLHELVKQPKPEWIDRAWLQIGLIRKSAGLF
                     EQAVAAFSALERAVPGSPLVRESQLQRALALIRLDRAAEAERLLRALATASIPQGDRA
                     ALELATIELERQQPDAAMTTLDAALKRFPESPLVPALHYRIAEVLLKKNRLEDAQARF
                     ERVVAVDPNDPWADDALQHAAQTALDRGDLAAARRLASSFATRFPQSPLKPELRLIEA
                     RAAGREGKHDEAATILKALLDEPADPKNGPPAATASEITQSARYELALCYRALGQADL
                     ATPILAGLAQGSKGQVAIDAKFLVGQSHLDAGRYTDAIPMLKAYLSTSPQGEVADVAM
                     AHLAVAHLGLSQLNDAERIVAELAERYPRTKILGPTRLRLAEAELAAHRADRACEQFQ
                     IVASVDRPNGELPAGSDAGKSNDSAEQSLRIQALAGLGKSLWELGKPAEAATAFATLL
                     RLAPNDPIAAEVALAHGRALEANEQPDAAKKAYLLVIERFAKSDQAQQAALSQARLLA
                     RSGRHVEAAHVFEQLVANPPARDSLQKAGVTPDVLLAELGSVLLDADKQAEADRVFSQ
                     LLKEYPESPRSADARFNLAESANLARNYSEVVRLLLPLATTKPVMPSAKPVADKSQSP
                     TAGPASTTDPIQRLLPAVLYRLGRTQVELNDLPAAAATLDRLLAEFTDNPYRREAQYL
                     RAEIALRQGDAALAERQFAALLDGPPPTDLGKEFLASVRMKRIECWVALKRWKDVLDQ
                     VQTRKGELAAGDPGVAALDYARGQALLGLGKLDEARAAFQAVIDARKDGELAAQAQLM
                     RGETYFHQDKFHEALRDFLKVDILHNAPRWQAAALLEAGKVYERLDHWADAAETYERV
                     LSKFASEPSAVEARERLAAVKGRAASTANPKL"
     gene            2714..3796
                     /locus_tag="VHS97_21700"
     CDS             2714..3796
                     /locus_tag="VHS97_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3450884.1"
                     /translation="MRRCKWIDRAFLTHAFGGVLAALAMGLAIGADDPHRHESADHHF
                     GHEAHAQAAGAEETAFFAEQLQSAIALARRGQGWLTAWYRNTLPADRVTWGGMLASAG
                     LALGVLFERLVRLQKHKIVPSEFTARFLDRLHEGKLDCGQALDHCELNPSPAARVALA
                     AVRRWGRPPADLERAVSMAHRVESERLRRNVGTLRRVAALTPLLGLLGTLFAAGRALE
                     AMPPGAPIYSNNASFGSQPGNDTEPSIAWGPTLAAALSPLSAGIIIATLALVAYDGLL
                     TRIEKLAGALDRLGAETIDAIALTAPISSPAVTLAHVAAHPIGATTDLAPGHSSMPAR
                     TPHQLSARRETRSDLSTRSGQEEMGF"
     gene            4244..4807
                     /locus_tag="VHS97_21705"
     CDS             4244..4807
                     /locus_tag="VHS97_21705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450885.1"
                     /translation="MAHPDSMAVTMRGSRALEAEFAENLRRNQAQLFAFIYSLVRDFA
                     DADDVFQQTSLILWKKYDQFIPSRSFVAWACGVARLEAAKFLRARDRRRGHFSTELSL
                     LLIDAQERLEHERLEEQRDALAECMTKLHQHDQDLLLACYGGSASVPEVARDWNRLPQ
                     SVYNSLRRIRRAVFDCVQRKLAGEMTG"
     gene            4804..6558
                     /locus_tag="VHS97_21710"
     CDS             4804..6558
                     /locus_tag="VHS97_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX3450886.1"
                     /translation="MKYLNDLPEELRDLFDAFCDGSIDDEQLKRLEAHILSDEAARLK
                     FVAYFHLHTELRFAARAKRVADSVLGTTGEPHRRATVWWRQWTIARPGRGPWMISAAA
                     CVLMALAYAAAWKWPGFVATRPQPVAESAAEPGRAVPAGGNWRPTAAESALAVVLKLD
                     DARWGTADGPNPAEGGVLPARRLHLLRGRATLAFLNGVVLTLEGPADLELISPDRVFC
                     QRGRLRARVPEGAEGFVVKSPASVVVDLGTEFGLNVEDDGRSRVMVFEGEAMAALFDG
                     VGSPQRTQRVDRSKAFDLDPRAGSIGEAVARPDGFVSASEMASPPLVLDPSYPDAVLR
                     SRPRGYWRFEQRTGDAVPNEVPDGPPLRASGPVVISGAPEGNGYALFKAGSPEQFLTT
                     DGLWELARDPGHAVELWFLSDNIRYASLVGLYPPPELNVPDQRGHIAHNFFVELTARN
                     RLYTLYKPASVRFLHRWPLDPRVGNNIVSEEMFAPRRWHHVVAQKNGDRMELYFDGVP
                     DRSMPLIADHQTVSCHLVIGRRTRDPLDQSDIRPFVGRLDELAIYEHPLSSEEVRSHF
                     RLAARTLAPDDRAARTAP"
     gene            6607..7704
                     /locus_tag="VHS97_21715"
     CDS             6607..7704
                     /locus_tag="VHS97_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3450887.1"
                     /translation="MPRRGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRLQCVNN
                     LKQLGLAAANYHSTNNCFPPGGFLNGKASGGQNGAYGSWSCFAFMLSNLEQTTIFNTI
                     NWSFGSGQGDAVTAYIQSTSLSARLNAMICPSASLPPSGVNPDGITTAISVPSVGDSY
                     FGSVGSSLEYDGRRTLGPPNGVFAFQGASAIGVQDVRDGTSNTIAFGEFRIGDFNNAK
                     ITIPSDVSDASSSAPAGVARNLPTMIAPFGTADGSNLVAWLQACAASLPTLHRSFVGD
                     TWAFGLMGRGLGNFVTAPNPKTPNCLDYTGQGDLDSAPIVVGPSSYHPGGANVGLCDG
                     SVRFLKDSTSLQTIWALGSRDQGEILDASSY"
BASE COUNT         1392 a         2654 c         2500 g         1418 t
ORIGIN      
        1 gtcgctggcg gcgctcggag aaccgggccg tgcggagatc gtgctacacg agctcgtcaa
       61 acaaccgaag cctgaatgga tcgaccgggc atggctgcaa atcggtttga ttcgcaaatc
      121 ggccggacta ttcgaacaag ccgtcgccgc gttcagcgcg ctcgagcggg ccgtacccgg
      181 cagcccgctc gtgcgtgagt cccagcttca gcgcgcgctg gctctcattc gactcgatcg
      241 cgcggcggag gctgaacgcc tgttgcgagc tctggcgact gcttccatcc cccaaggaga
      301 ccgagccgcc ctggaactgg cgaccatcga gctcgagcgc caacaacccg atgccgcgat
      361 gaccacgctc gacgcggcgc tcaagcgctt tccagagtcg cctttagttc ctgcgttgca
      421 ctatcgaatc gccgaggttc ttctcaagaa gaatcgattg gaagatgccc aggcacgatt
      481 cgagcgggtg gttgcggtcg atcccaacga tccctgggca gacgacgcat tgcagcatgc
      541 cgcccagact gccctggatc gcggcgattt ggcggcagct cggcggctgg ccagtagctt
      601 cgcgacgcga tttccccaga gcccactcaa gcccgaactt cggctgatcg aggcccgcgc
      661 cgccgggcgc gagggcaagc atgatgaagc cgccacgatc ctcaaagcac ttctggacga
      721 gcccgccgat cccaagaatg ggccgccggc cgcgaccgct tctgaaataa cccagtctgc
      781 ccgctacgaa ttagccctct gttaccgagc cttgggccaa gccgatctgg cgactccgat
      841 tctcgccgga ctcgcccaag ggtcgaaggg gcaggtcgct atcgatgcca agttcttggt
      901 cggccagtct catctcgacg ctggacgata cacagacgct atcccgatgc tcaaagcata
      961 tctgtccacg agtccccagg gcgaagtcgc cgatgtcgct atggctcatc tcgcggtggc
     1021 gcacttgggg ctcagccagc tcaacgatgc cgagagaatc gtggccgaac tcgccgagcg
     1081 atatccgcgc accaagattt tgggtccgac acgactgcgg ctggctgagg cggagcttgc
     1141 cgcccatcga gcggaccggg cgtgcgaaca attccagatc gtcgccagcg tcgaccgacc
     1201 gaatggcgag cttcccgcgg gttcggatgc cggcaaatcg aacgattcgg ccgaacaatc
     1261 gctgcggatt caagcgctgg cgggtcttgg caaatcactc tgggagctgg gtaagccggc
     1321 tgaagcggcc acggcctttg ctacgttact ccgtctggca ccgaacgatc caatcgcggc
     1381 cgaggtcgca ctggcgcacg gtcgcgcact cgaagccaac gagcagccag atgctgccaa
     1441 gaaggcctat ttacttgtca tagaacgatt tgcgaaatcg gatcaggccc aacaggccgc
     1501 attgtcccag gcccgactgt tggcccgtag cggccgacat gttgaggccg cgcacgtgtt
     1561 cgaacagctg gtagcgaatc cgccagcgcg cgactcactg caaaaggccg gcgtgacgcc
     1621 cgacgtcttg cttgccgagt tgggatcggt gcttctcgac gccgacaagc aggcggaggc
     1681 cgatcgagtt ttctctcaat tgctcaagga atatccggaa agtccgcgtt ccgcggatgc
     1741 ccgattcaac ctggccgagt cggccaacct cgcacgcaac tattccgaag tggtgcggct
     1801 gcttttgccc ctcgcaacga cgaagccggt catgccatca gccaaaccgg tcgccgacaa
     1861 gtcccaaagc ccaacggcgg gtccggcgag tacgactgac ccgatccagc gcctgctgcc
     1921 cgccgtgctg tatcggctgg gacgcactca ggtcgagcta aatgacttac ctgctgccgc
     1981 cgcgacgctc gaccgtctgc tggccgagtt taccgacaac ccgtaccgcc gcgaggcaca
     2041 ataccttcgc gccgaaatcg cactccggca aggagatgcc gctctggcgg aaaggcagtt
     2101 cgcagctttg ctcgatggtc cgccgccaac ggaccttggc aaggagtttc tcgcgtccgt
     2161 tcgcatgaag cggattgagt gctgggttgc cttgaagcgt tggaaggatg ttctggacca
     2221 ggttcagaca agaaaaggcg aattggccgc cggtgacccg ggggtcgccg cgctagatta
     2281 cgccagaggt caggctttgc tcgggcttgg caagctcgac gaagcgcgcg cggcgtttca
     2341 ggcggtcatc gacgctcgca aggacggcga gctcgcggcc caagcgcagt tgatgcgcgg
     2401 cgaaacctat tttcaccagg acaagtttca cgaggcgctc cgcgattttc tcaaagtaga
     2461 cattctgcac aatgcaccgc gctggcaggc tgccgcactt ctcgaggccg gaaaagttta
     2521 tgaacgactc gatcattggg ccgacgccgc cgaaacttat gaaagggtct tgagcaagtt
     2581 tgcgtcggag cccagcgcag tcgaagccag agaacgactg gcggccgtga aaggccgtgc
     2641 cgcgtcaacc gcaaacccaa aattgtaggg tgggccgatc ggcccaccgt gcgcttcggg
     2701 aaggattcca agcatgcgcc ggtgcaaatg gattgaccgc gcgtttctaa ctcatgcctt
     2761 cggtggcgtt ttggcggcgc ttgccatggg cttggcgatc ggtgccgatg atccgcatcg
     2821 ccacgaatct gccgatcatc actttgggca tgaagcccac gcacaagcgg ccggcgccga
     2881 agaaacggcc tttttcgccg agcaacttca atctgcgatc gcgctggcgc ggcgcggcca
     2941 gggctggctg accgcatggt atcgcaatac gctaccagcc gatcgcgtca cgtggggtgg
     3001 gatgctggcg tcggcgggcc tggcactggg tgttttgttc gagcgattgg ttcgactcca
     3061 aaagcacaag atcgtccctt ctgaattcac ggcgcgattt ctcgacaggc tccacgaggg
     3121 caaactggat tgtggccaag cactcgatca ttgcgagctc aatcccagcc cggcggcgcg
     3181 ggttgcgctg gcggcggtac gccgatgggg caggccgcca gccgatctgg agcgagcggt
     3241 ctcgatggca caccgcgtcg agagcgagcg cttgcggcgc aacgtcggca cgttgcgccg
     3301 cgttgccgca ctcacgccgc tcttgggact actcggtact ctgttcgccg ccggccgtgc
     3361 actcgaagcg atgccgcctg gggcaccgat ttactccaat aatgcatcct ttggatcgca
     3421 gcctggcaat gacacggaac cctcaattgc ctggggcccg acgctggccg cggcgctgtc
     3481 gccgctctcg gctggaatca tcatcgccac cctggctctg gtcgcgtacg acggtttgct
     3541 cacgcgcatc gaaaagctcg ccggcgcact cgaccgtctc ggtgcggaga ccatcgacgc
     3601 gatcgctctg acggcgccca tctcgtcgcc ggcggtcacg ctcgcacacg tcgcggcgca
     3661 cccgatcggc gcgacgaccg atttggcacc tgggcattca tcgatgccgg cgcggacacc
     3721 tcatcaactg tccgcgcgac gggaaactcg aagcgatttg tcgacccggt ccggtcagga
     3781 ggaaatggga ttttgatcaa gcaaagacgc gctcgcggct ggatcgctgg gatagcctct
     3841 ggcgtcgagt gggtcaacgt attctaactc cttaattcct cgtctccggt atggggttgg
     3901 ggccgcacgg ggcggccggg atgtcggggt gattgggttc accgtatccc gcatccctga
     3961 cgccatagag acaggcgagg tagtcgacgt gacgcgagtc ccgccggatt cggcctgtcc
     4021 ggggcccgcg aacggggtgg cccgaggggt catctgaccc gatcatcgca gcgcggcgat
     4081 cgatcgcagg acaccccgcg cggtgaccgg acgtgcgatc gctcgcgagg aattccgggg
     4141 cggtgctcca ttttgtggat tacaaatcgc ccgcgtcggg ggattactct ctgataccct
     4201 caaaaacccg acacgtcgcc gcaaaagggg tggtgtatgc ctcatggcgc atcctgatag
     4261 catggccgtc accatgagag gttcccgtgc cctggaggcc gagttcgccg agaacttgcg
     4321 ccgcaatcag gctcagctct tcgccttcat ttactcgctg gtgcgcgact tcgccgacgc
     4381 cgacgacgtg ttccagcaga cgagcctgat cctctggaag aagtacgacc agttcatccc
     4441 ttcccggagc ttcgtcgcct gggcctgcgg cgtggcgcgg ctcgaggccg cgaagttcct
     4501 gcgggcccgg gaccgccgtc ggggacattt cagcaccgag ctcagcctcc tcctgatcga
     4561 cgcccaggaa aggctcgagc acgagcgcct cgaggagcag cgggacgccc ttgccgaatg
     4621 catgacgaag ctgcaccagc acgaccagga tctcctgctc gcgtgttacg gcgggtcggc
     4681 cagcgtcccg gaggttgccc gggactggaa ccgactgccc cagagcgtct acaactctct
     4741 ccgccgcatc cggcgcgcgg tgttcgattg cgtccaacgt aagctggccg gggagatgac
     4801 tggatgaagt acctgaacga cctgccggag gagctccgcg acctgttcga cgcgttttgc
     4861 gacgggtcga tcgacgacga gcagttgaag agactcgagg cacacatcct gtccgacgag
     4921 gcggcacgac tcaagttcgt cgcgtatttc cacctgcaca ccgagctccg cttcgcggcc
     4981 cgggccaagc gggtggccga ctccgtactc gggacgaccg gcgagcctca ccgccgcgcg
     5041 accgtttggt ggagacaatg gaccatcgcg cgaccgggcc ggggtccctg gatgatctcg
     5101 gccgccgcct gcgtgctcat ggcgctggcc tacgcagccg cctggaagtg gccgggtttc
     5161 gtcgcaaccc ggccccaacc tgtggcagag tccgcggccg agccgggacg ggcggtcccg
     5221 gcaggcggca attggaggcc caccgcggcc gaatccgccc tggccgtagt gctcaagctg
     5281 gacgacgcgc ggtgggggac ggccgacggc cctaaccccg cggagggggg cgtcctgccg
     5341 gcccgtcgcc tccacctgct ccggggccgc gccacgctgg ccttcctcaa cggggtggta
     5401 ctcacgctgg aggggccggc cgacctggag ttgatctctc ccgatcgcgt attctgccaa
     5461 cggggaaggc tgcgcgcccg ggtccccgag ggggccgagg gtttcgtcgt caagtcgccc
     5521 gcatccgtcg tggtcgacct ggggaccgag ttcgggctga atgtcgagga cgacgggcga
     5581 tcgcgggtca tggtcttcga gggcgaggca atggcggcgc tgttcgacgg ggtgggctcc
     5641 ccccagagga cccagcgcgt ggaccggagc aaggcgttcg acctcgatcc ccgcgctggg
     5701 tccatcgggg aagccgtcgc gcggcccgac gggttcgttt ccgcctcgga aatggctagc
     5761 ccgccactcg tcctggaccc ctcctatccc gacgcggtcc tgcggtcgcg cccgcggggc
     5821 tactggcgtt tcgagcaacg gaccggcgac gcggtcccga acgaggtccc cgacgggccg
     5881 ccgctccgcg cgtccggccc cgtcgtcatc tccggcgccc cggagggcaa cgggtacgct
     5941 ctgttcaagg ccggatcccc cgagcagttt ttgaccaccg atggcctctg ggaactcgcc
     6001 cgcgatccgg ggcatgccgt cgagctctgg ttcctctcgg acaacatccg ctacgcctcg
     6061 ctcgtcggcc tctaccctcc cccggagctc aacgtgcccg accagcgtgg ccacatcgcc
     6121 cacaacttct tcgtggagct caccgcccgc aaccgcctct acacgctgta caagccggcc
     6181 tcggtccggt tcctgcaccg ctggccgctc gaccccaggg tgggcaacaa catcgtctcc
     6241 gaggagatgt tcgcgccgcg tcgctggcac cacgtcgtgg cccaaaagaa cggcgaccgg
     6301 atggagctct acttcgacgg ggtgccggac cgctcgatgc ctctcatcgc cgaccaccag
     6361 accgtgtcgt gccacctggt gatcgggcgg cggacccggg acccgctcga tcagagcgac
     6421 atccgcccct tcgtcgggcg gctggacgag cttgccatct acgaacaccc tctgtcctcc
     6481 gaggaagtcc gaagccattt ccggctggcc gcccgaaccc tggcccccga tgatcgggcc
     6541 gcgcggacgg cgccgtgatt ttcagtccct tttctccaag ttgtcccaaa cccagggagg
     6601 tatctcatgc cacgtcgtgg cttcacgctg atcgaattat tggtggtgat cgcgatcatc
     6661 gcggtgctga tcgcgctgct gctgcccgcg gtgcaggcgg cgcgggaggc ggcccgccgc
     6721 ctccagtgcg tcaacaacct caagcagctc ggcctcgcgg cggccaatta tcactcgacg
     6781 aacaactgtt tcccgcccgg cggattcctg aatgggaagg ccagcggcgg ccagaacggc
     6841 gcctacggct cctggagctg cttcgcgttc atgctctcca acctggagca aaccaccatc
     6901 tttaacacga tcaactggtc gtttggctcg ggccaggggg atgcagtcac cgcctacatc
     6961 caatcgacct ccctgtcggc acgtctcaac gcgatgatct gcccgtccgc gtccctcccg
     7021 ccgtcggggg tcaaccctga tggcatcaca acggcgatat cggtcccgtc cgtgggcgat
     7081 agttacttcg ggtccgtcgg atcgagcctg gagtatgatg ggcgtcgcac gttagggccc
     7141 ccgaatgggg tctttgcgtt ccaaggggca tccgcgatcg gtgttcagga cgtgagggac
     7201 gggacgtcga acacgatcgc gttcggcgag ttcaggatcg gtgacttcaa caacgccaag
     7261 atcaccatcc cttccgacgt ctccgatgcg tcgagttcgg caccggccgg cgtcgcgcga
     7321 aacctcccaa cgatgatagc gccgttcggc acggccgatg gctcgaatct cgtcgcgtgg
     7381 ctccaagcct gcgccgcctc ccttcccacg ctgcatcgat ccttcgtcgg cgacacttgg
     7441 gccttcgggc tcatgggccg tggtctgggt aacttcgtca cggccccgaa cccgaagacc
     7501 cccaattgcc tggactacac cggccagggc gacctcgatt ccgccccgat cgtggttggg
     7561 cccagcagct accaccccgg cggggcgaac gtcggcctgt gcgacggctc ggtccggttc
     7621 ctcaaggatt ccaccagctt gcagaccatt tgggccctgg gctcgcgcga ccagggtgag
     7681 atcctcgacg cctcgagcta ttgatgtctc tcgcctcagc ggcgggtggc tggggtccgt
     7741 cttcggaccc cggtccccac cgtccgcaac cgtgagcacc acggccttga acgccttcat
     7801 gagccctctc tgagcacatc ttgaagacag cactactcat caatgatgcc caacacggaa
     7861 gagcgtaatc gccagcctta gtcggccacg gaatcgctga cgaatgagac atatcacttc
     7921 ttcttcagca aatcctcggc ccgagacttc cgaggaagtc gagc
//
LOCUS       DATAQL010001259         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_888968, whole genome shotgun sequence.
ACCESSION   DATAQL010001259 DATAQL010000000
VERSION     DATAQL010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888968"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VHS97_21720"
     CDS             <1..754
                     /locus_tag="VHS97_21720"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF005734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX3450888.1"
                     /translation="VITDYGTEFDRVREGYKGPLFAEISPRAFSILVRTGSRLVQLRI
                     RRGSPGFSDTALRRLHEEVGLVDGPAGPKTIRNGLAFTVDVAGDEATGIVGFKARRHT
                     DVIDIDRSCHYDPLDFWEPVYSHRGEGIVLDPNDFYILASREAVVVPPDHAAEMMAYD
                     TFVGEFRVHYAGFFDPGFGAPESGGAGSRAVLEVRSHEVPFLIEHGQIVGRLVYERLI
                     ARPDKLYGTPIGSSYQSQGLALSKHFKPFVPR"
     gene            989..1165
                     /locus_tag="VHS97_21725"
     CDS             989..1165
                     /locus_tag="VHS97_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450889.1"
                     /translation="MPTIQQTIADKFLAKLAESEEVDASKIKQLRDLLTDDKKPAPDD
                     FVKIFTIPAGGDLK"
     gene            1162..2142
                     /locus_tag="VHS97_21730"
     CDS             1162..2142
                     /locus_tag="VHS97_21730"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450890.1"
                     /translation="MIKLETAHIEEVRGIRKLDIDFRKETFTISGPNGSGKSGVIDAI
                     EFGLTGQIGRLTGRGTKGLSVSEHGPHVDKTKFPGAAFVELRVFLTELGKSVTINRKI
                     SSPKKPKITPADEDVKAVLAEVAEHPEIALSRREILRFILVEPTKRSEEIQTILKLDK
                     IGHIRATLNTAQNRLQSAQNAAGTQMQSNRDALLLHLQIATLRAADVLDAVNKRRVIL
                     GLPEIADLTADTRLDAGLSAGAAVPDFNKVSALRDLTALSEAAEGFPTLAAAEAAAIV
                     ANLSRLEIDPGLLAALQQRSLSKRASISWTGRNARFAIAPGKPNNICVTT"
     gene            2178..2336
                     /locus_tag="VHS97_21735"
     CDS             2178..2336
                     /locus_tag="VHS97_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450891.1"
                     /translation="MRQELLDNGTAVAQATIRVANLLSPVQKLATAEGDADLGLLLTR
                     WKADLEDL"
     gene            2352..>3095
                     /locus_tag="VHS97_21740"
     CDS             2352..>3095
                     /locus_tag="VHS97_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450892.1"
                     /translation="MDGLTGLKDRLSGEWVGVPKTFREGLKSLTTKIEAKPDQSATID
                     AQTFLTTAQLRLGDYREGMRRNKAAEIALATAKAAYIAYCTVMDDELDALYDDVQADF
                     STYYRAINEGDESRFTAKLTPSAGRVDFDVNFYERGLFPPGAYHSEGHQDGMGVCLYL
                     ALMKRLFGNRFKFALLDDVVMSVDVGHRRQFCKLLKTYFSDTQFIITTHDRLWAEQMK
                     SAGLVTGKTSLTFHGWSVDTGPVVEANEEV"
BASE COUNT          625 a          950 c          946 g          574 t
ORIGIN      
        1 ggtcatcacc gactacggca ccgaattcga ccgcgtccgc gagggctaca agggtccgct
       61 ctttgccgag atctcgccgc gcgccttcag catcctggtg cgcaccggct ctcgcctggt
      121 gcaattgcgc atccgccgcg gttcgccggg gttcagcgat acggcgctgc gccggctgca
      181 tgaggaggtc gggctggtgg atggtccggc cgggccgaaa acgatccgca acgggctcgc
      241 ctttaccgtc gatgtcgccg gcgacgaggc gaccgggatc gtcgggttca aggcgcggag
      301 gcataccgat gtgatcgata tcgaccgcag ctgtcattac gacccgctcg atttttggga
      361 gccggtctac tcgcaccgag gcgaggggat cgtgctcgac cccaatgatt tctacatcct
      421 ggcatcgcgc gaggcggttg tggtgccgcc cgaccacgcc gccgagatga tggcttacga
      481 cacttttgtc ggcgaattcc gggtgcatta cgccgggttt ttcgaccccg gtttcggcgc
      541 ccccgaaagc ggtggggccg gcagccgcgc cgtcctcgag gtgcgctcgc acgaagtgcc
      601 gttcctgatc gagcacggcc agatcgtcgg ccgcctcgtc tacgagcggc tgatcgcccg
      661 gcccgacaag ctctacggca ccccgatcgg ctcctcctac cagagccagg gcctggcgtt
      721 gtcgaagcat ttcaaaccat tcgtcccgag atgacggggc cgcccgtcac cctcccactg
      781 acgcgggtcc cttcctctcc ccgttcgcgg cctctcggtc agaagctatc cgccgtctca
      841 tggcgcgacg ctgaatgcgg cgagcaatgg gcctaagaag cttggtgggg cgccaccctt
      901 caggcggtga caaccgccgc ttgattgggc gtcccgcagg gccacatcca gtcattaccg
      961 ctgaccacat tttgcaacgt gagcgcactt gccgactata cagcagacca tcgcggataa
     1021 gttcttagca aaactcgctg agagtgaaga agtagacgcc agcaaaatca agcagcttcg
     1081 agatttattg accgacgata agaagccagc gccagacgat ttcgttaaga tctttacgat
     1141 acctgctggc ggggatctga agtgattaag ctggaaacgg cacacatcga ggaagtgcgt
     1201 ggcattcgga aactcgacat cgacttccgc aaggaaacct ttacgatctc cgggccgaac
     1261 gggtctggca aaagcggcgt gatcgatgcg atcgagttcg gcctgacggg ccaaattggt
     1321 cggttgaccg gaaggggcac caagggcctg tccgtttccg agcacgggcc gcacgtggat
     1381 aagacgaagt tccctggcgc ggccttcgtc gagctacgcg tgttcctgac cgaactcggc
     1441 aagtcagtca cgatcaatcg caaaatctct tcgccgaaaa agccgaagat tacgcctgct
     1501 gatgaggacg tgaaggccgt cctggccgaa gttgcagaac acccggagat tgcgctttcg
     1561 cgtcgtgaga tcctgcggtt catcctcgtc gaaccgacga agcgctccga ggaaatccag
     1621 acgatcctca agctcgacaa gatcggccac atccgggcca cgctgaacac ggcccagaat
     1681 cgccttcaat ccgcgcagaa cgccgcaggc acgcagatgc agtccaatcg ggatgcactc
     1741 ctcctgcacc ttcagatcgc caccctgcgt gcggcggatg tgttggacgc agtcaacaag
     1801 cggcgagtta tcttgggcct ccccgaaatc gccgatctga cggcggacac gcgactcgac
     1861 gcggggcttt ctgctggggc ggcggtgcct gacttcaata aggtctctgc gttgcgcgat
     1921 ctcacggccc tgtcggaagc cgcggagggt tttccgacgc tcgcggcggc ggaggcagcc
     1981 gcaatcgtcg ccaatctgtc gaggcttgaa atcgatcccg gcctgctggc cgcgttgcag
     2041 cagcggtcgt tatcgaaaag ggcctcgatc tcgtggacgg gccggaatgc ccgctttgcg
     2101 atagcccctg ggaaaccgaa caacatctgc gtgaccacct agaggccaag ctcgcgaaat
     2161 cggagcaagc ccgaaaagtg cggcaggagc ttctcgataa cggtaccgca gtcgcacagg
     2221 cgaccatccg ggtcgcgaac ctgctgtctc cagtccagaa gcttgccacg gcagagggcg
     2281 atgccgatct cggactgctg ctgacaagat ggaaagcgga tctcgaagac ctgtgatcta
     2341 agctcgcaaa cgtggacgga ctgaccgggc tgaaggaccg tctgtcgggc gagtgggttg
     2401 gtgtcccgaa gaccttccgg gaagggttga agagtctgac cacgaagatc gaggccaagc
     2461 ccgatcagag cgcgacgatc gacgcgcaaa ccttcctcac taccgcgcag cttcggctcg
     2521 gcgactaccg ggagggaatg cgcaggaaca aggccgcaga aattgccctg gcgaccgcga
     2581 aagccgcata tatcgcctac tgcacggtga tggatgacga gctagacgcg ctctacgatg
     2641 acgtgcaggc cgactttagc acctattacc gagcgatcaa cgagggcgac gaatccaggt
     2701 ttacggccaa gctcactccg agcgcaggtc gggtcgactt cgacgttaat ttctacgagc
     2761 gcggtctgtt tccgcccggc gcataccata gcgaaggcca ccaggacggg atgggcgtct
     2821 gcctttacct cgccttaatg aaacggctgt tcggcaaccg tttcaagttt gccttgctcg
     2881 atgacgtggt gatgtcggtc gatgtcggcc atcgtcggca gttctgcaag ctgctgaaga
     2941 cctacttctc agacacgcag ttcattatca ccacccacga tcgcctctgg gccgagcaga
     3001 tgaaatcggc gggtctggtg actggcaaga cctctctcac ttttcacggt tggagcgtcg
     3061 acacggggcc agtcgtcgag gccaacgaag aggtt
//
LOCUS       DATAQL010001260         4842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1652717, whole genome shotgun sequence.
ACCESSION   DATAQL010001260 DATAQL010000000
VERSION     DATAQL010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652717"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            517..1842
                     /locus_tag="VHS97_21745"
     CDS             517..1842
                     /locus_tag="VHS97_21745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEX3450893.1"
                     /translation="MRVILDVLEGPLKGRSFMFERHDTFIVGRSRFVHCPMPEDMALS
                     RDHFMVEINPPLCELRDLGSTNGTFVNNNRVDRVRLVSGDVVAAGQSVFRVQVESEQV
                     LSSSDGNASGPTLTETVSTGQIRCAGCGVIAPSNLTIIGPLAGTTGEKVEWWCVMCRS
                     EAAGLPQPVPYYTTLRELGRGAMGVVYQARHNLTGQMVALKLIMPETATTRAAIDRFQ
                     REISVISQLRHPNIVELYEQGMTRGQFWFAMEYVAGTNLETLAKADPGRYPIKQACRL
                     TCQMLKGLEQAHHLGFVHRDIKPENILIAQGPDGLAVKISDFGLAKSFRGVGLSGLTF
                     SGEMRGTVPFMPPEQMLDFKTVTPLADLYATAATLYYILSCQYMYDEPSGGGDLIRML
                     LEEAPIPIRDRRADVPPALGDVIAKCLARDPKDRYPDATSMRRALKPFC"
     gene            complement(1873..2445)
                     /locus_tag="VHS97_21750"
     CDS             complement(1873..2445)
                     /locus_tag="VHS97_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3450894.1"
                     /translation="MSSVWLVAMMSLSAITTLSRLADIGPAPPTIMVNSSGKLFDLTS
                     LKGKVVIVSFVYTTCNGTCPATTLTLSRVQKVLRQAKLWGTSVEFVSISLDPERDTPD
                     VLKRYAELFNADLANWNFLTGSRSDVQSVIAAWGMWARIGPSGALDHPSRIFLLDPQG
                     HQREIYNLEFLKTETVIQDVKELLAEVSKN"
     gene            complement(2457..3653)
                     /locus_tag="VHS97_21755"
     CDS             complement(2457..3653)
                     /locus_tag="VHS97_21755"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450895.1"
                     /translation="MISRRDLLIRSAGAGVGSGILSLGVPVSGLWRQAAAAALPCGDL
                     PILVVVELTGGNDGLNTVIPYADDIYHKNRPTLRIEPDKVLKLDDHVGLHPSLKELHS
                     LWESGDLAVLQGVGYPNPNRSHTRSMEIWQTGVVEAAPPAGWLGRVADVNSTFRICHV
                     GPESVPMAVRGRTSIPQALASLSDYQLVANAGLPTGPQGDQAGDPLVGDIRQRLISAR
                     ELAARLAALPAGPSGSQSASVPETLEGRLATIHRLIEADLPLRVYYTSQGGFDTHAAQ
                     RFVHQDLLRLVSEAVAGFLKALKASRLDERVVVLVFSEFGRRLKENTSSGTDHGAAAP
                     VFLAGRPVKGTLIGPPPSLAELDDGGDPRFTTDFRDVYATLLSRWLAVDPDAILGRRD
                     VSLSLF"
     gene            complement(3670..>4842)
                     /locus_tag="VHS97_21760"
     CDS             complement(3670..>4842)
                     /locus_tag="VHS97_21760"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEX3450896.1"
                     /translation="GQSTADFVATVAAMEESAQRRPSMERVQMLWLYRLIFTPFPLVE
                     VMTLAWHGHYATSQAKVNAPELMLAQQNAQRALWRAPISQLHRRMLNDGAMRRWLDGL
                     NSTKAQPNENLGREFLELFALGEGRYTERDVREAARALTGWREVDFQQHRDQLDTGEF
                     DDGAKTILGETGNWGLDDLVEIACRQPAAATHIARRLYHTFISNTDTPSADLLAPLAD
                     AMRTKDDVDVARGIDFVLRSRLFQSTECRRSCVKSPVDFAIGAIRSLEVFAPPPDLVD
                     LEIYLTKMGQRLFFPPSVAGWPRGLAWLGGQELVARVNFAAWINELSRQGNESHFDSL
                     VRRYGLKTPETQLEALATLLLGAPLTLAQSSLAGRQSGGLVRTVGGLLSLPEAQLG"
BASE COUNT         1028 a         1459 c         1413 g          942 t
ORIGIN      
        1 cgcgtttcgc attccaagat atcgtagagc caatggagca ccattacggt gcgcatttcg
       61 cagtccaagg tctcggagtg ccaatggagg gcgcttaggc ccgcccaggg accagaactt
      121 aagagctcga tttggcaagc atcaagaacg atgtcactct ttcaatgaca acagcgaaag
      181 gaaaacgacg gaaatgaatc ctcgccgtat tgtgctgcca cattgcaggg tcttgtcgcg
      241 tacttaatct aagggtggag gatacgtcct gacgcatgag ccgcgtatca cttgaacgtt
      301 gcttgggtgg aggaacttcc ttgagcgtgc gttcaggaag atcattgtta cgaagcccag
      361 cacattccgc ggtctccact cgtagaactc tggtcgatgc ccgatggcgg ttgccgagga
      421 gattgtcgct ataatggatc cacgagatac gacgaactat ccgacaataa ccccacaaat
      481 aacgtaatag cccggtgaaa ttgacgggac ggattgatgc gtgtgattct ggatgtgctc
      541 gaaggaccgc tcaaggggcg ctcgtttatg ttcgagcgcc acgacacgtt tatcgtaggt
      601 cgttcgcgat tcgttcactg tccgatgccc gaggacatgg cgctatcacg cgaccacttc
      661 atggtcgaga tcaatccgcc tctatgtgaa ttacgcgacc tcggtagcac caacggcacc
      721 ttcgtcaaca ataatcgcgt cgaccgggtg cgacttgtct ccggtgatgt ggttgcggcc
      781 ggccaaagcg tgtttcgagt ccaagtcgaa tcggagcagg tcctgtcgtc gtccgacggg
      841 aacgcctctg gaccaactct gaccgagacc gtctcgacgg gtcagattcg gtgcgcaggt
      901 tgcggagtga tcgctccttc gaatctgaca attatcggtc cgctggccgg aacgaccggc
      961 gagaaggtcg agtggtggtg cgtcatgtgc cggtcagagg ccgcagggct tcctcagccc
     1021 gttccgtact atacgacgct acgcgagctg ggccggggcg ccatgggcgt cgtctaccag
     1081 gcgcgccaca acctcaccgg ccagatggtc gccctcaagc tgatcatgcc cgagacggct
     1141 accacgcgtg cggcgatcga tcgcttccaa cgcgagattt cggtcatcag tcaactgcgc
     1201 catcccaata tcgtcgaatt gtacgaacaa ggcatgaccc gcggccagtt ctggttcgca
     1261 atggaatatg tcgcgggcac taacctggaa acgctcgcca aggctgaccc cggccgctat
     1321 ccgatcaaac aggcttgccg tctcacatgc cagatgctca aaggactgga gcaagcccac
     1381 cacctcggct ttgtccaccg cgacatcaaa cctgagaata tcttgatcgc ccagggtccc
     1441 gatgggctcg ccgtcaaaat ctccgacttc ggcctggcca agagcttccg cggcgtcggg
     1501 ctttcgggcc tcactttctc cggcgaaatg cgcgggactg tccctttcat gccccccgaa
     1561 cagatgctcg atttcaagac cgtcacgccg ctagccgacc tctatgcaac ggctgctact
     1621 ctttactata tattgtcatg tcagtacatg tacgacgaac ccagtggtgg cggggatcta
     1681 atacgcatgc tgctggaaga ggcaccgatc cccattcgcg atcgccgtgc cgatgtgccg
     1741 ccagccctgg gtgatgtaat cgccaagtgt ctggcccgcg atcctaaaga ccgttatccc
     1801 gatgcaacct cgatgcggcg ggccttgaag cctttctgtt gagtaggcag tagagttcgc
     1861 cgaagacacg aattagttct tcgaaacttc ggccagcaac tccttcacat cttgaattac
     1921 ggtttcggtc ttgaggaact cgagattata gatctcccgc tggtgccctt gcgggtcgag
     1981 caggaagatc cgcgacggat ggtccagcgc accggaagga ccgattctgg cccacatccc
     2041 ccaggcggcg atgacggatt gaacgtcaga acgagagccg gtcagaaaat tccaattggc
     2101 taggtcggca ttgaagagct cggcatagcg tttcaagacg tcgggtgtgt cgcgctcggg
     2161 atcgagcgaa atcgacacga actcgaccga tgttccccac agtttagcct gccgtagcac
     2221 cttctgaacg cggctaagcg tcagtgtggt cgcagggcac gtaccattac atgtcgtata
     2281 gacgaacgac acgatcacca ccttgccttt caacgaggtg aggtcaaaga gcttgccaga
     2341 cgagttgacc atgatcgtcg gcggcgcggg gccgatgtcc gccagccggc tcagagtagt
     2401 gatcgcactc aaagacatca tcgcaacgag ccaaacacta gacacgggta actccttcaa
     2461 aacaacgaga gagacacgtc gcgccggccc aggatcgcat cgggatcgac agccagccat
     2521 cgactgagca acgtggcata aacatcgcgg aagtcggttg taaagcgagg atcgcctccg
     2581 tcatcgagtt ccgccagact cggcggcggg ccgatcaagg tccccttgac tggcctgcct
     2641 gccaagaaca cgggggcggc tgcgccgtgg tcggtgccac tcgaggtgtt ttccttaagt
     2701 cggcggccga actcggaaaa caccaaaacc acgacccttt catcaagtcg gctcgctttc
     2761 agagccttga gaaacccagc cacggcttcc gacaccaggc gcagcaaatc ctgatgaacg
     2821 aagcgctggg cggcgtgcgt gtcgaaacca ccctgtgagg tgtaatagac gcgcaatggc
     2881 aagtcagcct cgatgagtcg gtgaatcgtc gccaatcggc cttccagtgt ttccggtacg
     2941 gaggcagatt gcgagccgga tgggccagcg ggtagcgccg ccaggcgcgc tgccaactcc
     3001 cgggcagata taagccgctg gcgaatatcg ccgaccaggg gatcaccagc ttggtcgcct
     3061 tggggtcccg tgggcaatcc ggcgtttgcc acgagctgat agtccgacag gctggccaac
     3121 gcctgcggaa tcgacgtgcg gcctcgcact gccattggca cggactctgg cccgacatga
     3181 caaattctga acgtcgaatt cacgtccgcc acacggccca accatcctgc cggcggtgcg
     3241 gcttcgacca cgcccgtctg ccagatctcc atcgagcgtg tgtgtgagcg attgggattg
     3301 ggatagccca ccccttggag caccgccagg tcgcccgact cccaaagcga gtggagctcc
     3361 ttgagcgacg ggtgtaaacc gacgtggtcg tccagtttca agaccttgtc aggctcgatt
     3421 cgcagggtcg gccgattctt gtgatagatg tcgtcggcgt aggggatgac cgtgttcaag
     3481 ccgtcattgc cgccggtcaa ttccaccacg accaggatcg gcaagtcccc gcacggaagg
     3541 gcggccgcgg ctgcctgacg ccaaagacct gataccggca cgcccagcga caagatcccc
     3601 gaccccacgc ccgcgccagc cgaccgaatg agaagatcgc gtcgagatat cataacaaat
     3661 tgctcctctt tatcctagct gtgcctcggg gagagaaagc aaaccgccaa cggtgcgaac
     3721 aagacctccg gactggcgcc ctgcgagcga actctgcgcc agggtaagcg gcgcgccaag
     3781 caatagtgtc gccagcgctt cgagttgggt ttccggtgtc ttcaggccat atcgcctcac
     3841 caaactgtca aagtgacttt cattgccttg ccgcgacagc tcgttgatcc aagccgcgaa
     3901 gttgacccgt gccacgagtt cttgcccgcc cagccacgcc agcccccgtg gccagcccgc
     3961 cacactcggc ggaaagaaga gccgctggcc catcttggtc agatatatct ccaggtcgac
     4021 caagtccggc ggcggagcaa acacctcaag gctacggatg gcgccgatcg cgaaatcgac
     4081 cgggctcttt acgcagctcc ttcggcattc cgtcgactga aaaagacgcg agcgaagtac
     4141 gaagtcgatc cctctggcaa cgtcgacatc gtctttcgtt cgcatcgcgt cggccagggg
     4201 cgcaagcaaa tcggccgaag gcgtatcggt attggagatg aacgtgtggt agagccgccg
     4261 ggcaatgtgt gtcgcggcgg ctggctggcg acaagcgatt tcgaccagat cgtcgagacc
     4321 ccagttgcct gtctcgccca gaattgtttt agcaccgtcg tcgaattcgc ccgtatcgag
     4381 ctggtctcgg tgctgctgaa aatcgacctc acgccagccc gtgagagccc gggcagcctc
     4441 gcgcacgtcg cgctcggtgt atcgaccctc gcccagtgcg aaaagctcga gaaactctcg
     4501 acccaggttt tcgttgggct gggccttcgt actattgagc ccgtcaagcc agcggcgcat
     4561 agcgccgtca tttagcatcc ggcggtgcaa ttggctgatc ggagctcgcc acagcgcgcg
     4621 ctgggcgttt tgctgggcaa gcatcagttc cggagcgttg accttggcct ggctggtggc
     4681 atagtggccg tgccaggcca aggtcatgac ctcgacgagg ggaaacggcg tgaagatcag
     4741 acggtaaagc cagagcattt gaaccctctc catagaaggc cggcgctggg ccgactcttc
     4801 catggcggca acggtggcga cgaaatctgc ggtggactgg cc
//
LOCUS       DATAQL010001261         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3485535, whole genome shotgun sequence.
ACCESSION   DATAQL010001261 DATAQL010000000
VERSION     DATAQL010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485535"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1645
                     /locus_tag="VHS97_21765"
     CDS             <1..>1645
                     /locus_tag="VHS97_21765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3450897.1"
                     /translation="KTNVKGPIHITTPAEEQATFSLPEGYAINLFASEVEFPDLEDPV
                     SMTFDAKGRLWVGTMPSYPMYLPGTKPNDKVLILEDANDDGKADRCTVFADGLHLPIG
                     IELGDGGVYLSQMPNFMFLKDTNGDDRADTRELIMHGFDTADSHHAMHAYTWDPGGAL
                     YWNEGTFHYSQVETPYGPRRCNEAGVFRWEPKTWKFDVFVSYGFANPWGHYFDRWGQN
                     FVADASGGANYYGTAFSGQVVYPQKHGGMKQFFPMQWRPTCGCELVSSRHFPDDTQGD
                     YLLNNDIGFQGILRYRVKDEGSGFVGAPVEPLLRSSDPNFRPVALQFGPDGALYVVDW
                     FNPLVGHMQHSLRDPNRDHTHGRIWRITYPKRPLVEKPRIAGATVTELLDLLKSYEDR
                     TRFRARRELRERPEQEVLSALDKWKNGLNTSDPEYWRQMLEALWLHQSLDDIDMPFLK
                     QMLSCHEPRARAAATRVLCYWRDRIGETIELLRKQVNDDHPRVRLEAIRALSFFDGDQ
                     VAKAQEIALESLLFPQDEYLEYTLNETNKTLDRRTKDQGK"
BASE COUNT          350 a          515 c          469 g          311 t
ORIGIN      
        1 aagaccaacg tcaagggacc gatccacatc acgaccccag ccgaggaaca agcaacgttt
       61 agcctccccg agggctacgc gatcaatctg ttcgcttccg aagtcgagtt ccccgacctg
      121 gaagatccgg tgtcgatgac cttcgacgcc aagggccggc tctgggtcgg aacgatgccg
      181 tcgtacccga tgtatctgcc cggaaccaag cccaatgaca aagtcttgat cttggaagac
      241 gccaacgacg acggcaaggc cgaccgctgc acggtctttg ccgacggctt gcacttgccg
      301 atcgggatcg agctcggaga tgggggcgtc tacttatcgc agatgcccaa tttcatgttc
      361 ctgaaagaca ccaacggcga cgatcgcgcc gacacgcgcg agctgatcat gcacggcttt
      421 gacacggctg attcgcacca cgcgatgcac gcctacacgt gggaccccgg cggcgccctg
      481 tactggaatg aagggacatt tcactattcg caggtagaga cgccatatgg accacgacgg
      541 tgcaacgaag cgggcgtctt ccgctgggaa cccaagacct ggaagttcga tgtcttcgtg
      601 tcgtacggat tcgccaatcc ctggggccac tacttcgacc gctggggtca aaacttcgtg
      661 gccgacgcct ctggcggcgc caactattac gggaccgctt tctcaggtca ggtcgtctac
      721 ccccaaaagc acggcggcat gaaacagttc ttcccgatgc agtggcgccc aacgtgcggc
      781 tgcgagctgg tcagcagccg acatttcccc gacgacacgc agggagacta cctcctcaat
      841 aacgacatcg gttttcaggg gattctccgg tatcgcgtca aggacgaagg ctcaggattt
      901 gtgggcgcgc cggtcgagcc gctgttgcgt tcgtcggatc ctaacttccg tcccgtcgcg
      961 ctccagttcg gcccggatgg tgcgctctat gtcgttgatt ggttcaaccc cttggttggc
     1021 cacatgcagc actcgctgcg cgatcctaac cgcgaccata cccatggcag gatctggcgt
     1081 atcacctatc ccaagcggcc gctggtcgaa aagccgcgga tcgccggtgc caccgtgact
     1141 gagctcctgg atctcctcaa gtcctacgaa gatcgcaccc gctttcgtgc tcgccgcgag
     1201 ctacgcgagc ggcccgagca ggaggtgttg tcggccctcg ataaatggaa aaacggcctc
     1261 aacacaagcg atccggagta ctggcgccaa atgctcgagg cactctggct gcaccagagt
     1321 ttggacgata tcgacatgcc gtttctcaag caaatgttga gctgtcatga gcctcgtgcg
     1381 cgcgccgcag ccacccgcgt gctttgctac tggcgcgatc ggatcggcga aacgatcgag
     1441 ctgttgcgca agcaggtaaa cgacgatcac ccgcgcgtac ggctcgaagc cattcgggct
     1501 ttgagcttct tcgacggcga ccaggtggcc aaggcgcaag aaattgcact cgagtcgctg
     1561 ctctttcccc aggacgagta tctggaatac accctcaacg agaccaacaa gaccctcgac
     1621 cggcggacta aagatcaagg taaga
//
LOCUS       DATAQL010001262         4703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3030021, whole genome shotgun sequence.
ACCESSION   DATAQL010001262 DATAQL010000000
VERSION     DATAQL010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3030021"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VHS97_21770"
     CDS             complement(<1..291)
                     /locus_tag="VHS97_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450898.1"
                     /translation="MSRCGCLILVAACLTSTCAARVLAQASNPPYDSGLDIVGDPAGG
                     DMPADGEAGVSSSSTASQPSPFGGPWNSRPKLTGDWGGLRDQLRDHGFTFDIS"
     gene            complement(550..1005)
                     /locus_tag="VHS97_21775"
     CDS             complement(550..1005)
                     /locus_tag="VHS97_21775"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HEX3450899.1"
                     /translation="MSTSEVSELLYKVTLNITGMKEYGVGLAALTAREVTPPVEGARF
                     DVAFEGTATGPKLNGTIEGIDYVRVRPNGRFELHIHETIRTEDGQNISAHGEGVANLR
                     PEGGIADVRVNMTLFASSREYTWVNQLPVRGIGTLDLDKQVIEVAAYCA"
     gene            complement(1002..2036)
                     /locus_tag="VHS97_21780"
     CDS             complement(1002..2036)
                     /locus_tag="VHS97_21780"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3450900.1"
                     /translation="MRNRAYMVLTPLVLLGTTITAQAWDRGKVERFITLPPGQAHPEG
                     IDVDRDGNVYVGTVDASKPETSEGTLVVFDPKGKYLRTVSVAGSSRLLLDLRFHPQTG
                     KLLVIDYLGAKVLTVDPTTGTSSVFMTVTGKNPGLNDMAFDTAGNVYVTDSHEGIIWK
                     VSKDGGEGTAWVESPLLTPTRPPPSIGANGLAFNNEQTALFVANTANDTIVKIPVAGS
                     PLEPGTPEVLVNRAGGGPDGLLIDEHDNLWIACNQSNEIMVLEPKQGRVIAKLGDFGG
                     IDKDGAPIGFLWSNTLVFHGDDVLVTNASLDLGAAISQKMRTVDGPWAAAVKLHTVSK
                     IKKNIPSAPQ"
     gene            complement(2049..4373)
                     /locus_tag="VHS97_21785"
     CDS             complement(2049..4373)
                     /locus_tag="VHS97_21785"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX3450901.1"
                     /translation="MRNKQATSTKQSGPSTSAGAWGLRGTWIFALIASLVFAATPFAQ
                     MPQSPWKKAAPFPEPDEELYGVAVNGKLYVIGGWGEGKARGANYEYDPATDKWTKKTS
                     MPRPAHHAALAAANGKIYVIGGFVVPEKIQIPVGGAWEPIDDVWEYDPAADSWKTLAP
                     LPGKRGAAVAVEAGGKIYVIGGATTVEGSKDPFFTFFGPCRVLATNDVYDPATNKWES
                     RTPMAVPRNHAFAGAVDGKIYVIGGRTGHAFILSATNTDAVEEYSPVADMWSAPKERM
                     PTPRSGGGFGTDGRRIYVAGGEVTTKELVGAFRAIEAYEPATNSWIALPSMPMPRHGV
                     AGAVIGNRFHLVSGMVQSAGAMAFLDPNLATHTAAHDVLELQFNPSPPAEKSGAAAPS
                     GGPKKLYARYNVNSPEGQKMLAKYARAIELMRELPEYDPHSWTWWWNTHWIKGFPAAL
                     WDYSRKHKTEVIAALPPGSQADAEAVWNGCQAHPYNPSNPEQYQQWYLLPWHRLMLYE
                     FEGVIREVLHDEDFSLPYWNPVTGNPDDLSLPAVFRDPGSSLYDGTRWPWVNGGERID
                     TPFWGWLSLDALNEKLYIDSPTGNLGFNPRLDMNPHFFTHIALGGDMADFSTVGADPL
                     FYLHHANLDRIWESWNRLGNSNPTDPKYLNRTFAFGDRSGKRVDLPVSAGDRTAQLGY
                     EYDSYEKPPHTGPQASRDAVSRDAAIKSLYDRAHGGPHGAQHAPSSRGSSEKTAQHGC
                     GSRCSYPDFGSIAKTALTSGGHDAVDITVPDGQP"
BASE COUNT          828 a         1469 c         1436 g          970 t
ORIGIN      
        1 gctgatgtcg aaggtaaagc cgtggtcgcg gagttggtcg cgcagcccac cccagtcccc
       61 agtcaacttt ggccgggaat tccagggacc gccgaacgga gaaggctgtg aggcagtgct
      121 gctcgaggac accccggcct cgccgtcggc gggcatgtcg ccgcctgccg ggtcgccgac
      181 aatgtccaag ccgctgtcgt agggcgggtt cgacgcctgg gccaagacgc gagcggcgca
      241 ggtcgatgtc aggcacgcgg caaccaggat caagcaaccg caacgcgaca ttgaaaagtt
      301 cccggaccat cgattcaagc acattccggt cactgtggaa ccctcggtat attcttcttg
      361 atcttcgaga ccgtgtgcag cttcaccgca gcaggccacg ggccatcgac ggtccgcata
      421 ttctgcgaac tagcggcgcc ggctctgctc cctcttatat cccacccttt cggtttcaca
      481 tggttcatgg tccatcactc ttgatccgtt cggtgtcgta ggagtggctc cgctatgccc
      541 tggcggagct caggcgcaat acgccgcaac ctcaataact tgcttgtcga gatccagtgt
      601 cccgatgccc ctcaccggaa gttggttcac ccacgtgtac tcccttgaag aagcaaacag
      661 tgtcatgttt acgcggacat cagcgatgcc gccttctggc cgaagattgg ccactccctc
      721 gccgtgagca gaaatatttt gcccatcctc cgtcctgatc gtttcatgaa tgtggagttc
      781 aaaccggcca ttcgggcgga ctcggacgta atcaattccc tcgatagtgc cattcagttt
      841 cgggccggtc gctgtgccct cgaaagcgac atcgaatctc gcgccttcaa ctggtggcgt
      901 cacttcccgg gccgtcaatg ccgcaagccc gacgccgtat tccttcatgc cggtgatgtt
      961 caaggtcacc ttgtatagca actcgctcac ttcactcgtg ctcactgcgg agcgctcggt
     1021 atattcttct tgatcttcga gaccgtgtgc agcttcaccg cagcagccca cgggccatcg
     1081 acggtccgca ttttctgcga aatagcggcg ccaagatcga gcgaggcgtt ggtaacgagg
     1141 acgtcgtcgc cgtgaaacac gagggtattc gaccagagaa aaccgatcgg cgcgccgtcc
     1201 ttgtcgatgc cgccgaagtc gccgagcttg gcgatcaccc ggccttgctt cggctccagc
     1261 accatgatct cgttggactg attgcacgcg atccagaggt tatcgtgctc gtcgatgagg
     1321 agtccgtccg gtccaccccc cgcgcggtta accaagacct ccggcgtgcc gggctcgagc
     1381 ggtgagccgg ccaccggtat tttcacgatc gtgtcgttag ccgtgtttgc gacgaacaac
     1441 gcggtctgct cgttattgaa cgctagccca ttggcgccga tggagggtgg gggccttgtt
     1501 ggcgtgagga gcgggctctc gacccaagcg gtcccctcgc cgccgtcctt gctgactttc
     1561 cagataatcc cctcgtgcga gtcggtcacg tacacgttgc cggcggtgtc gaaggccata
     1621 tcattgaggc cgggattctt ccccgtgacc gtcatgaaca cggacgaggt gcccgtcgtg
     1681 ggatcaacgg tcaggacctt cgcgcctagg taatcgatca cgagcagctt gcccgtctgg
     1741 ggatgaaagc ggaggtcaag caggagtcta cttgagccgg cgacgctgac ggtccgcagg
     1801 tacttgccct tcgggtcgaa cacaacgagt gttccctcac tcgtctccgg tttgctcgcg
     1861 tccacggtgc cgacgtagac gttgccgtca cgatccacgt cgattccctc gggatgcgct
     1921 tggcctggcg gcaacgtgat gaagcgctcg accttgccgc ggtcccaggc ctgcgcggtg
     1981 atggtcgtcc ccagcagcac caaaggtgtc agcaccatgt aggctctatt tctcatgacg
     2041 ccctctcctt aaggctggcc atcagggacc gtgatgtcga cggcgtcgtg gccaccactc
     2101 gttagtgcgg tcttagcgat cgatccaaag tcgggataag agcagcgcga gccgcacccg
     2161 tgctgagccg tcttctcgct cgagccccgg ctgctcggcg catgctgagc accatgcgga
     2221 ccgccgtggg cacggtcgta gagcgacttg atagcggcat ctctggatac ggcatctctg
     2281 gaggcttggg gccctgtgtg gggcggcttc tcatagctgt cgtattcgta gccgagctgc
     2341 gcggtgcggt cacccgcgct gaccggcaga tccacgcgct tgccgctcct gtccccgaag
     2401 gcgaacgtgc ggttgaggta tttgggatcg gttggattgc tgttgccgag ccggttccag
     2461 ctttcccaaa tgcggtccag attggcgtga tggagataaa agagcgggtc agcgccgacc
     2521 gtggagaagt cggccatatc gcccccgagt gcgatatgag tgaagaaatg agggttcatg
     2581 tcgagcctgg gattgaaccc caggttgcca gtcggcgagt cgatgtacag cttctcgttg
     2641 agggcgtcca gactgagcca gccccagaag ggtgtatcga tccgttcgcc cccattgacc
     2701 cagggccagc gcgtgccgtc atacagcgag ctgcccgggt cccggaacac ggcagggagg
     2761 gagagatcgt cggggtttcc ggtgaccggg ttccagtagg gaagcgaaaa atcctcatcg
     2821 tgcagcacct cgcgaatgac cccctcgaac tcataaagca tgagtcgatg ccagggcagg
     2881 aggtaccact gctgatattg ctcagggttg gaggggttgt aggggtgggc ctggcagccg
     2941 ttccacacgg cctcggcatc ggcttgagaa cccggcggga gtgccgcgat gacttccgtt
     3001 ttgtgtttcc tggagtaatc ccacagggcc gccgggaagc ccttgatcca atgggtattc
     3061 caccaccagg tccatgaatg ggggtcgtat tccggcaact cccgcatgag ctcgatcgcc
     3121 cgcgcatatt tcgcgagcat cttttggcct tcggggctgt tgacgttgta gcgcgcgtag
     3181 agcttctttg gacccccgga aggcgccgcc gccccgctct tctcagccgg aggactcgga
     3241 ttgaactgca gctccagcac gtcgtgcgcc gccgtgtggg ttgcgagatt cgggtcgagg
     3301 aacgccatcg cccccgcgga ctggaccatc ccgctcacca ggtgaaagcg attgccgatc
     3361 accgcgcccg cgacgccgtg acgcggcatc ggcatagagg gcagcgcgat ccatgaattg
     3421 gtcgccggct cgtacgcctc gatggctcga aacgcgccga cgagctcttt ggtcgtcact
     3481 tcgcccccgg ccacgtagat cctgcggccg tccgtgccaa agccgccgcc gcttcggggt
     3541 gtcggcatcc gctctttcgg agcactccac atgtcggcca cgggactgta ctcctccact
     3601 gcatccgtgt tggtggcgga caggataaag gcgtggcccg tgcggccgcc gatgacgtag
     3661 atcttgccgt cgacggcacc tgcgaacgcg tgattgcgcg gcacggccat cggcgtgcgg
     3721 ctctcccatt tgttggtggc gggatcgtac acatcattgg tggcgagaac ccgacacggt
     3781 ccgaagaacg tgaagaacgg gtccttggac ccttccactg tggtggcgcc gccgatcacg
     3841 tagatcttgc cgccggcttc gacggccacc gcggccccgc gcttgccggg cagcggcgcc
     3901 agcgtcttcc aggaatcggc cgccggatcg tactcccata cgtcgtcaat cggttcccat
     3961 gcgccgccga ctgggatctg gattttctcc gggacaacga agccaccgat cacgtaaatc
     4021 ttgccgttgg cggcggccag ggccgcatgg tgcgccggcc gcggcatcga agttttctta
     4081 gtccatttgt cggtggccgg atcgtactcg tagttggcgc cccgagcctt gccttcgccc
     4141 cacccgccaa tcacgtacag tttgccgttg acggcaaccc cgtagagttc ttcatccggc
     4201 tccgggaacg gtgccgcctt tttccaggga gattgcggca tctgagcgaa tggggtcgcc
     4261 gcgaaaacca ggctggcgat caaggcaaag atccacgttc cccgcaggcc ccacgcgcct
     4321 gcgctcgtgc ttggtcctga ctgctttgtt gaagtggctt gtttgttacg catttcgaaa
     4381 acctcctctg cagttgggct ttggtgcggc tactagcgta agctagcgag ccgaaccgcc
     4441 gggaagggcc taccgttcgc actatccttg caacccgtcg gggcaggtga accggtcgat
     4501 tcgtatttca actcttaact atctggcaaa ttggacctga gatgcaattc gcgcaattgc
     4561 ttcggggtca ccgttgaagg cgcgcccatg agcaaatcct cggcgcgctg gttcatcgga
     4621 aaaagcacga cctcgcgcaa gatgggcgat cagccgagga cgaccaccat tttcccgccg
     4681 gcctcatggg cctccatgac gcg
//
LOCUS       DATAQL010001263         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1960256, whole genome shotgun sequence.
ACCESSION   DATAQL010001263 DATAQL010000000
VERSION     DATAQL010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960256"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VHS97_21790"
     CDS             <1..234
                     /locus_tag="VHS97_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450902.1"
                     /translation="ATVGGRSPASQGPLVSLAERDFVYIRNEGDGSEQLFNQRSDPQE
                     IDDRSRFESMRPVLEGFRNSLDLIRRTSASSRR"
     gene            680..>1566
                     /locus_tag="VHS97_21795"
     CDS             680..>1566
                     /locus_tag="VHS97_21795"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3450903.1"
                     /translation="MLSESAARWPKEGEMPFLLGACEQALGRPDAAEAAWSRVPTDSP
                     FAGHAAMLRGRLLMKRHRFAQAEQLLPAALRASGQHAIEARETLVLLFKLEGRFDEVR
                     SLVQDGWDSYPDRFGLLRQLANLDSINPVPIEKIWPALEQAAASSPDDDRIWLGRANL
                     AIRTGEFAKAQNWLDDCLRRRPDDAAVWKSRLDLAVATGDVARAKEAMGHLRPNGVRP
                     EEVLTLRAWFAARSGDEERERQTLEKLLELVPGQLQAVERLAELELHAGRPESAARLR
                     ARKAELDRAKIHYEMLVTKP"
BASE COUNT          283 a          480 c          505 g          298 t
ORIGIN      
        1 gcaacggtcg gagggcgatc accggcttca caagggccgc tcgtctcact ggccgaacgt
       61 gacttcgttt acatccgcaa tgagggcgat ggcagtgagc aactcttcaa ccagcgcagt
      121 gatcctcaag aaattgacga ccgcagtcgg ttcgaatcga tgcggcctgt cctcgaggga
      181 ttccgcaaca gtctcgattt gatcaggaga acttcagcgt cgtcccgacg ctgaagtttg
      241 attcctggcg ggcgggctgt attaccgcga tgtcgctatc cgtgggtctg acaaatcttt
      301 gagctcccaa ggcacccacc gaagatgacc cactcgcttc cagtctgggg agccctaatg
      361 cgactgatct tgcgatcgcg ttgtctgatt caaactgttc aagaagctaa tggccgccat
      421 gcggcgaagc ttgcggacgc cgtcgggtcc atgctacttt tatcgtcgtc agacacgtcg
      481 cttgcacttc gatcaatgcc tgtctcgggt cgaatgggag ggttgaatgc gtcggcgtcg
      541 attgttcatg ctgctcggcg tcgtcgcgct ctttgccgca tggtgaacgg cgcgcggagt
      601 tgactcttgg cgctatcgga cgaacctgga gcaggccaag agatggatcg agtccggctc
      661 gcccgccgag gctaggcggt tgctttcaga gtcggcagcg cggtggccca aagaaggaga
      721 gatgccgttc ctcctaggtg cctgtgaaca agcccttggt cgtcccgacg ccgccgaagc
      781 ggcctggtca cgcgttccga ccgattcgcc cttcgccggg cacgcggcca tgttacgcgg
      841 ccggctgtta atgaagcgcc accggttcgc ccaggccgag cagttgctcc ctgccgccct
      901 acgggcaagt ggccagcacg ccatcgaggc gcgtgagacg ttggtcctct tgttcaagct
      961 cgaggggcga ttcgacgagg tgcggtccct tgtccaggac ggctgggata gctaccccga
     1021 ccggttcggc ctgctccggc aacttgcaaa tctcgactcg atcaacccgg tgccgatcga
     1081 gaagatctgg cccgcgctcg agcaagcagc cgcgagctct cccgacgacg atcggatctg
     1141 gcttggccgg gcgaacctcg ccatccgcac gggcgagttt gccaaagccc agaactggct
     1201 cgacgactgc ttacggcgac gcccggacga tgccgcggtg tggaagagcc ggctcgacct
     1261 ggccgttgcg accggcgacg ttgccagggc taaggaagcg atggggcatc tacgtcctaa
     1321 cggtgtcagg cctgaggagg tcctgacctt gcgcgcctgg ttcgccgcgc gatccggcga
     1381 cgaagagcga gagcggcaga cgctggagaa attgctcgag ctcgtgccgg gtcagcttca
     1441 ggccgttgag cggctggccg aactcgaact gcacgccggg cggcccgaaa gcgctgcccg
     1501 gctgcgcgcg cgcaaggctg agctggatcg ggccaagatc cattacgaga tgctcgtgac
     1561 gaaacc
//
LOCUS       DATAQL010001264         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2113824, whole genome shotgun sequence.
ACCESSION   DATAQL010001264 DATAQL010000000
VERSION     DATAQL010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113824"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VHS97_21800"
     CDS             complement(<1..553)
                     /locus_tag="VHS97_21800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450904.1"
                     /translation="MEFLRLVVVLVMGCALLAVGVVLAQPGPGSGERDRLDKLFRAGN
                     YKDAFDGYRALALDAKSKSDTIRLEFERAIACLAPLGRIDEIDDFREAAVAVHQGSRQ
                     LLEAAAASCLDETHFGTVVAGKFHRGGGQGGGRSVRSNERDRSRALQLLVQGLDLVRL
                     DADRTAAGGYYLTLARALMSGREQ"
     gene            complement(661..2181)
                     /locus_tag="VHS97_21805"
     CDS             complement(661..2181)
                     /locus_tag="VHS97_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450905.1"
                     /translation="MSIKYRRIPLPGSFLLTILLTAEVLVLAYVQSPAERFMRYIAFD
                     SGVDLTMQNLMARGFRPTIDFGCIYGLLPLLINRGWYGVAGLTPGAFRAEVILGTCLT
                     AWGMARFATALRVGPAGVALIVLAMPDVLFPSYSTIVHVLEPALLVHGLAEQAKGRRG
                     TALALATASCFVKPAMGYFYGLVLLISIGAASKEQGRSYWLRSLAPAAIVGTALALMV
                     AAVFGVAPMLEMLFPAKGAAVYRLGRYGFFHGVGSEFWALPGTGIWRYFRYEVGFWII
                     GSVWLVAGGMVALARLVRGAATNLRAQDDEIVACCAALHIVFVTCLFGQRMTWTYYFA
                     ILILGLAAMGKGSRTRVWITWFLVVLLLVNDRSKLLNTYREWSAGAPTAITLGLWATP
                     AERSEWRTVLELTEGQEPVLLSGVDASLPLTPRFAPPVAGYFCPGLTLPVEIRRKVDQ
                     LSAARMIVTTLPSQGEGFDYWPELAAALDSCELIFEGGTFRVYRRIRPPSGQSRRP"
     gene            complement(2377..3147)
                     /locus_tag="VHS97_21810"
     CDS             complement(2377..3147)
                     /locus_tag="VHS97_21810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3450906.1"
                     /translation="MSATLQPICDRQTQCKCCGATALPYGVVDFNKNCEIYRHNALGV
                     SGVPIYYHRCPACRFIFTTAFDHFTHEDFQSHIYNEEYPLIDPDYQVARPHGNAALVT
                     RLFPGKKPRRVLDYGGGNGVMAESLRAAGFPHVETFDQFVEQYSARPAAGFDCVICFE
                     VVEHSTDPARIFADMDEFLTDPGLILFSTLVQPADIDSQGLNWWYAGPRNAHVSLYTT
                     ESLASVVRPLGFNLGSFSESYHILFREMPDFARHFIRA"
BASE COUNT          742 a         1009 c          979 g          550 t
ORIGIN      
        1 cctgctctcg gcccgacatg agcgcacgag ccagggtgag atagtagccg cctgcggccg
       61 tgcggtccgc gtccagccgt acaagatcaa gtccttgaac caagagctgg agggcgcggg
      121 accgatcgcg ctcgttcgag cgcaccgaac ggccaccgcc ctgaccaccg cctcggtgaa
      181 acttgcccgc gacgacggtc ccgaagtgtg tttcatccaa gcaactcgct gccgcagcct
      241 cgagtagttg ccgactccct tggtggaccg cgacggcggc ctcgcggaag tcgtcgatct
      301 cgtcgatccg gccgagcggt gccaggcacg cgatcgcgcg ctcgaattca agacggatgg
      361 tatcggactt gctcttggca tcgagcgcca gcgcgcggta gccgtcgaag gcatccttgt
      421 aattccccgc cctgaacagc ttatccagcc gatcgcgttc gcccgaccca ggtcctggct
      481 gggcgagcac gacacccacg gccagtaacg cgcaacccat caccaggacg acaaccagac
      541 ggagaaattc catggtagcc tcgcttggtt tgaaactcgc ccgacgactc agcgggagat
      601 atggggcgac ttcaccccac tataacgtac cacgattgct tttcttagtg ctcgaaccgc
      661 tcatggccga cgcgattgcc ctgacggagg ccggatgcgg cggtaaacgc ggaacgtacc
      721 accctcaaag attagctcgc aactgtcgag ggcggcggca agctcgggcc aatagtcgaa
      781 tccttccccc tgagagggca gcgtcgtgac gatcattcgc gcggcggaga gctggtcgac
      841 cttacgacgg atttcgaccg gaagcgtcag ccctgggcag aaataaccgg cgactggcgg
      901 cgcaaaccga ggtgtgagtg gcagcgaagc atcgacgccg gacaaaagca ccggctcttg
      961 tccctctgtc aattcgagta ccgtgcgcca ttccgaccgc tcggccggcg tggcccagag
     1021 accgagcgtg atagccgtcg gcgctccggc ggaccactcg cgataggtat tgagcagctt
     1081 ggagcgatcg ttgaccagca ggagtaccac taaaaaccag gtgatccaca cgcgggtgcg
     1141 gctcccttta cccatcgcgg ccaggcccaa gatcaggatg gcgaaataat aggtccaggt
     1201 catccgttgg ccgaacaagc aggtgacaaa gacaatatgt agggccgcgc agcaggcgac
     1261 gatttcgtca tcctgtgctc gtaggttcgt cgccgctccg cggacaaggc gcgccagagc
     1321 gaccattccg cccgccacaa gccagacgga accgattatc cagaagccga cctcgtaccg
     1381 aaaatagcgc cagattcctg ttccggggag tgcccagaac tcactaccga ccccgtgaaa
     1441 aaaaccatat cggcccagcc ggtagaccgc ggcccccttc gcgggaaaaa gcatttcgag
     1501 catgggggcg acgccgaaca ccgccgccac catgagcgcc agcgcggtgc cgacgattgc
     1561 cgccggcgcg agcgaccgca gccagtacga ccgcccctgc tctttcgagg ccgctccgat
     1621 cgagatcagg agcactaacc cgtagaagta gcccatcgcc ggcttgacga agcaagacgc
     1681 cgtggcgagc gccagcgcgg tcccacgccg ccccttggcc tgctccgcca gaccgtgcac
     1741 cagcaaggcc ggttcgagga cgtgcacgat cgtgctatac gatggaaaca gcacatcggg
     1801 catagccagg acgatcaggg cgaccccggc tggcccaact cgaagcgcag tcgcaaatcg
     1861 ggccatgccc caggcagtca ggcacgtccc cagaatcact tccgcccgga acgcgccggg
     1921 cgtcaacccg gccacgccgt accaaccccg gttgatcaag agcggtaaga gaccgtaaat
     1981 gcagccaaaa tcgattgtcg gccggaaccc gcgggccatc aagttttgca tcgtcagatc
     2041 gacgcccgag tcaaacgcga tatagcgcat gaaccgctcg gcgggcgact ggacgtaggc
     2101 gagaacgagg acctcggccg tgagcaagat tgtgagcaag aaagaacccg gtagcggaat
     2161 gcggcgatac ttgatcgaca tggcgaaagg ggcaacacct tgaaggaccg accactgaac
     2221 atgcgagctg aatgagccta tcagatgagg gcaactaacg acaatgcggc ggagctttcc
     2281 cgcccttcac aagcggggga aagccacgcg aaattggcta ctttttcgga gacaatttgc
     2341 ctggtggcgt cgggaccggc tcttgctcag ctcgcttcaa gccctgatga aatgccgggc
     2401 gaagtcaggc atctctcgaa acaagatgtg atagctctcg ctgaaagaac cgaggttgaa
     2461 gcccaaaggc cgcacgaccg acgcgaggct ttctgtagta taaagagaaa catgagcatt
     2521 ccttggtcca gcgtaccacc agttcaaacc ttggctgtcg atatctgcgg gctgcaccag
     2581 ggtcgagaac aggatcaagc cggggtccgt caagaactcg tccatgtcgg cgaaaatgcg
     2641 cgcggggtca gtggaatgct cgacgacctc gaagcaaatg acgcaatcaa agccggcggc
     2701 aggacgggcg gagtattgct cgacaaattg gtcgaacgtc tcgacgtgag gaaatcccgc
     2761 agcacgcagt gattcggcca tcacgccgtt gcccccgccg tagtcaagca cgcgccgtgg
     2821 tttcttgcca gggaacagcc gagtcaccaa agcagcgttt ccgtggggac gcgccacctg
     2881 atagtccgga tcaatgagtg gatactcttc gttgtagatg tgcgactgga aatcttcatg
     2941 cgtgaaatga tcgaaagccg tcgtgaagat aaagcggcag gccgggcacc ggtggtagta
     3001 aatcggcacg cccgaaacac ccagcgcgtt atggcggtag atctcgcagt tcttgttgaa
     3061 gtcaacgact ccataaggca aggcagtggc gccgcagcac ttacactgcg tttgccggtc
     3121 gcaaatgggc tgcaaggtag cactcatcga ttatctccag aattgacgcc ggccagtctc
     3181 tggagctcga cttcgatgtg accgccgatt ttgacggctg atcttttaag actttgtact
     3241 tattgcggca agagcaggat ctgagtttac ctgctcgccg
//
LOCUS       DATAQL010001265         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3485960, whole genome shotgun sequence.
ACCESSION   DATAQL010001265 DATAQL010000000
VERSION     DATAQL010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485960"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3619)
                     /locus_tag="VHS97_21815"
     CDS             complement(<1..3619)
                     /locus_tag="VHS97_21815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3450907.1"
                     /translation="MSGRNGQIGDYMDAIVGDTDERRFLQSTPWIIDAHPQDPDPLVT
                     ALRQQTYRLSELPRQPRFTLLTVLRDCRLQHLKELILSVRCQSYQNWEMVLVDDGSTT
                     RKRLEFAQTWADCDERIHVRDIEIPFGPSWAKNLAMEEATGDFLIVTDGDGVLHPMAL
                     GVFARHLNEDPNVNFIFANEAEIDHSSTGLTNFLTKPPLDLFTLLRVPYIGRLCAMGR
                     DLVDRATQGGGPIFRDAYDGIEEHDLSLRLALTSGFNSRHVPLFTYYRRAGSHGQTRL
                     TDQKLVERRRRLAEEFIPRVYPGSSWSLTVRKDRDPLASTSIWLKDVPGWTCPKLLVV
                     IPFKDQVETTIQCLESIERQEHQLDVVVALVNNQSSEEETLPRLRLWIESPRAAKYQI
                     LNHDGAFNFARLNNEAVARFGGDRDLICLMNNDVELSMPHTLETLAMQLLADRSIGFV
                     GIKLLYPGGKEIQHGGVRVGELIYGSGYYEIKHDCNAQGFVDAERISMGVTFACAMTR
                     RQTFESLGGLDELYLPNGYGDVDMCLRAIDAGFRNYYLGSLSAIHHESKSRGSTNEDI
                     EITFLHQRNGQTIASWRHWHLNRSYRYAWPLVVLRWDDPRSSLLDELSQPMPAAFHGE
                     GTIPSPVPLRYRLADRLNGVLKLVLGPLHGTLRTGVSQTFQVIRAVRRPGVVVSAGRR
                     LLRPMPVVGNVARKGLRRARKIRYHWRVSTGLVKALWRKPEALRLLAMGLGRGGGHGL
                     RQSIRMLVPVANASHPTHQEWFDATRPSRLLLARLRRRRWPAQAPKFTVVTPVYNVKE
                     KWLRGAVESVIAQTYPHWEMICVNDSSTAWHIRPTLDELAARDPRVKVIHCASNRGVS
                     VATNLGLDQATGDYIAFMDHDDHLEPHALHRFAEAVLEDHPDMIYSDEAVTEVDINRV
                     QHVTCRPSFSYDYYLSYPLFVHLVAARTEIVRRIGGLNEAMTISQDIDYGLRLIEASR
                     HITHIPEVLYRWRTHPGSLGHQQKNKVYAMTRGALERHFDRIGVAVDFDDRVHFNFRD
                     LRFALTSSARVAIVIPTKDQPDRLRACVASLLCTVPPELAEIIILDRSSDERVTQSVL
                     EDLSGRHRILRVEGELNSSAILNCGAAASTAATHYLFLHDDTEAFANGWLEHMLGYAH
                     RADVGVVGGTLLYPDETIQHAGAVIGLNGTHDHVLKRNLFHVSEVGRCCGPNGMLLAS
                     RD"
     gene            4271..>4523
                     /locus_tag="VHS97_21820"
     CDS             4271..>4523
                     /locus_tag="VHS97_21820"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3450908.1"
                     /translation="MASHTKARGTRHAVDDAFDAVISVPVLEQVRDPFTAAKEITRVL
                     KPGGKLFCCVRFLQPFHGYPNHYYNMTHVGLASLFGGLKV"
BASE COUNT          966 a         1284 c         1316 g          957 t
ORIGIN      
        1 cgtcgcggct tgcgagcagc atgccgttgg gaccacagca acgcccgact tctgatacgt
       61 ggaaaagatt acgcttaagc acatgatcgt gcgttccgtt taagccgatc acggctccgg
      121 catgctgaat cgtctcgtca ggatagagca acgtcccccc gacgactcca acgtcggccc
      181 gatgtgcgta gccgagcata tgctcaagcc agccgttggc gaacgcttcg gtgtcatcat
      241 ggaggaaaag gtaatgagtt gcggccgtgc tcgcggcagc gccgcagttt aagatcgctg
      301 aagaattgag ctcaccttca acgcgcagaa tccgatgacg cccggacaaa tcctcgagca
      361 cggattgcgt gaccctttca tccgacgacc ggtctaggat gatgatttcg gcaagctcag
      421 gcggcacggt gcataaaaga ctggcgacgc aggctctcag tcggtccggc tggtctttgg
      481 tcggaatcac gatcgcaacg cgagcggatg acgtcagggc gaatctcagg tctcggaagt
      541 tgaaatgcac cctgtcgtcg aagtccacag ccacgccgat tcgatcgaaa tgccgctcga
      601 gtgcgccccg agtcatcgca tagactttgt tcttctgctg atggcccaga ctccctggat
      661 gggtccgcca gcgatagagt acctccggta tatgcgtgat gtggcggctg gcttcgatca
      721 gacgtaaacc atagtcgatg tcttgcgaga tcgtcattgc ttcgttcaga ccgccgattc
      781 gccggacgat ctccgtccgg gccgcgacca aatgcacgaa aagagggtag ctcaggtaat
      841 agtcgtaaga aaacgacggc cgacacgtga cgtgctgaac gcggttgatg tcgacttcgg
      901 tgaccgcctc gtcgctgtag atcatgtcgg gatgatcttc caaaacagcc tcggcaaacc
      961 ggtggagagc gtgaggttcc aggtggtcat cgtgatccat gaatgcgata tagtcaccgg
     1021 tggcctggtc gagtcctaaa ttggtggcga cactgacgcc tcggttgctc gcgcagtgga
     1081 ttacctttac tctcggatcg cgcgccgcaa gttcgtcgag agttggccgg atgtgccatg
     1141 ccgtcgaact atcattgacg cagatcatct cccaatgcgg atatgtttga gcgatgacgc
     1201 tctcgacggc ccctctcagc catttttcct tgacgttgta gactggagta acgactgtga
     1261 atttcggagc ctgtgccggc cagcggcggc ggcgcaaccg cgctaaaagt aggcgcgagg
     1321 gtcgcgtcgc gtcaaaccac tcttgatgag tcgggtgcga ggcgttcgcg acgggaacga
     1381 gcattcggat tgactgacgc aggccgtgcc ctcctccccg ccccaaaccc atcgccagga
     1441 gccgaagagc ctccggtttg cgccagagag ccttgaccaa tcccgtggaa acgcgccagt
     1501 gatacctgat cttgcgggct cgccgcagac ctttgcgtgc gacattgccg acgacaggca
     1561 tcggcctcag gagtcggcgc ccggcgctca caacgacgcc gggtcgtcgg accgcacgaa
     1621 tgacctgaaa agtttgagac actcccgtcc gcaaagtacc gtgcaatgga ccaaggacga
     1681 gcttgagcac cccattaagc cggtctgcca gtcgatagcg gagcggaaca ggactcggta
     1741 tcgttccttc accatggaat gccgcaggca tcggttgaga gagttcgtcc agcagcgaag
     1801 aacgcggatc atcccaacga agtacgacga gtggccaggc atagcgatag gatcggttca
     1861 agtgccagtg tcgccacgac gctatggtct gcccattgcg ctgatgaagg aaggttatct
     1921 cgatgtcctc gttggtcgag cctcgtgatt tcgactcgtg atgaatggcc gagagactgc
     1981 ccaggtagta attgcggaaa ccggcgtcga tcgcccgcag gcacatgtcg acatcgccat
     2041 agccattggg tagatagagc tcgtcgaggc cccccagact ctcgaaagtt tgtcgtcgag
     2101 tcattgcgca ggcgaacgtc acgcccatgc tgattcgttc ggcatcgacg aacccctggg
     2161 cgttacagtc atgcttgatc tcgtaatatc ctgatccgta gatgagttcg ccgactcgca
     2221 cgcccccatg ctgaatctct ttgcccccgg gatataacag tttgattccg acaaaaccga
     2281 tggaacggtc ggcgagcagt tgcatggcca acgtttctag cgtatggggc atggacaatt
     2341 caacgtcgtt gttcatgagg caaatcaagt cgcggtcacc accaaaacgg gcgacggcct
     2401 cgttgttaag cctggcgaaa ttgaaagcgc cgtcgtggtt gaggatctga tacttggccg
     2461 ctcgcggcga ctcgatccac aagcgcagtc gcggcagagt ctcttcctcg ctcgactggt
     2521 tgttgacgag tgccacaacc acgtcgagct gatgttcctg gcgctcgatt gattcgaggc
     2581 actggatcgt cgtctcgact tggtctttga acgggatgac aacgagcagc ttggggcagg
     2641 tccagccggg cacgtccttg agccagatgc tggtcgatgc gagaggatcg cgatccttgc
     2701 gcaccgtcaa cgaccagctt gagccgggat agacacgcgg tatgaactct tccgccaggc
     2761 gacggcgccg ttcgactagc ttttggtcgg tcaaccgcgt ttgtccgtgc gaaccggcac
     2821 gtcggtagta agtgaataac ggaacatgcc gggaattgaa gcctgaggtc agtgcgagcc
     2881 ggaggctaag atcgtgctct tcgatcccgt cgtacgcgtc acgaaagatt ggtccgccgc
     2941 cttgagtggc tcgatcgact agatcgcgac ccatagcgca taacctgccg atgtagggaa
     3001 ctcgcaagag cgtaaacaga tccaagggag gcttcgtaag gaagttggtc aagccggtgg
     3061 aagaatgatc gatttccgcc tcattggcga agatgaagtt gacgttcgga tcttcgttga
     3121 gatgacgggc gaaaactcca agcgccatcg ggtgcagaac accatcgccg tcggtgacga
     3181 tgagaaagtc gccggtcgcc tcttccatcg ccaggttctt cgcccaactt ggtccgaagg
     3241 gaatttcgat gtctctcaca tgaatccgtt catcacagtc ggcccacgtc tgggcgaatt
     3301 cgagccgttt tcgcgtggtg ctgccgtcgt cgaccagcac catttcccag ttttggtacg
     3361 attggcaacg taccgacaag atcagttctt tcaggtgctg caaccgacag tcacgcagca
     3421 ccgtcagtaa cgtgaatcga ggctggcgtg gaagctcgct taatcgataa gtctgttgtc
     3481 gcagcgcggt cacgagtggg tccgggtctt gggggtgcgc atcgatgatc cacggagttg
     3541 actggaggaa ccgcctttcg tcggtgtcac cgacaatcgc atccatgtag tcaccgattt
     3601 gaccattcct accggacatg gcagactccc tcccgacttt cgttgagaca acacgatcga
     3661 gcatgggcgc ggccgcgccg aaccagtgtg tgcatcaccg tcgaacaaag tgagcccgtc
     3721 gcacgccccc ttgtccttgg cggcgtgtca gctgccttct ttgtatcggc gccgtgggcg
     3781 tctgtcgtca tcccgcttgc gcaaatggca aaatcagcac tcggatcgac caccagcact
     3841 cgacgggaaa acacggctat cttcgctaat ttaccgcgtt ccgaccggga ttgagtgcca
     3901 ggaattgtcg cggacgcgat cttctttacc atgcggaaga tgctggcacc ggccatgttg
     3961 gaggtgagga actcctgtct cccagcgatc agtagaaaaa ttcaaatgga aaaactctta
     4021 ctcgccggta aaagaggttt gtgatcgccg attatggaca tagcgctcat tcttggcaat
     4081 ctcgtccttg ggagtgccaa atcgcctggt ctgcctagac tcgcagcttt ccgcccgtgc
     4141 cgatcaacga tccggcgaat tggcccgcaa cacggaagct cgaaacttga agtcgccgtc
     4201 aataagggct tggcgaacca cggagccaag tccggtaaga atgcgttccc gcgtatgcca
     4261 ggagaacgga ttggcttctc atactaaggc tagaggaact cgccacgctg tcgacgatgc
     4321 cttcgatgcg gtgatttctg tcccggtcct cgagcaagtt cgagacccgt tcacagcggc
     4381 caaggaaatc acgcgcgtac tgaagccggg cggaaagctc ttctgctgtg tccgcttcct
     4441 ccaaccattc cacgggtatc caaatcatta ctacaacatg acccacgtgg ggttggccag
     4501 cctgtttggc ggcttgaaag tgc
//
LOCUS       DATAQL010001266         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2726373, whole genome shotgun sequence.
ACCESSION   DATAQL010001266 DATAQL010000000
VERSION     DATAQL010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726373"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>1555
                     /locus_tag="VHS97_21825"
     CDS             40..>1555
                     /locus_tag="VHS97_21825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3450909.1"
                     /translation="MPNIQAQLPPELKLDLLFDQSTFVRAAVEGVLKEGAIAAGLTAL
                     MILLFLGSWRSTLIVAVSIPLSILTGIIILWGMGQSLNTMTLGGMALAVGILVDDATV
                     EIENVHRNLGMRKPIRRAILDGAAQIAVPAFVSTLAICIVFVPVVFLTGPAASLFTPL
                     ALSVVFAMMTSYFLSRTLVPTMVLYLLPPEVELYAEGEHGGSGGGPIWRFHRAFNRQF
                     ERLRRSYERFLSWALAHRAVTLTGLLGFALGSLALTPFIGRDFFPAVDAGQFRLHVRA
                     PAGTRIEETERIFGQVEEVIREIVPAHERELILDNMGMSPSFTVRAYIDNGTVSDADG
                     EILVSLKEEHGPTAGYVAKLRRELPRRFPDCMFFFQPADITSQILNFGLPAPINVQVV
                     GVNAAGNLAAAKKLRREVSKIRGIADVHLHQITDQPTLRLDVDRIMASELGLYQQNVA
                     GSVLVSLSSTSQTTPNYWVNPLNRVNYVLAVQTPPDRMDSVDAMMNTPILNYQAG"
BASE COUNT          268 a          514 c          456 g          317 t
ORIGIN      
        1 tcctccacac taagtgtcgt caaccgcgtt cgcgccgctt tgccgaacat ccaagcgcaa
       61 ttgccgcccg agctaaagct cgacttgctg tttgaccaat cgacctttgt ccgcgcggca
      121 gtcgaggggg tcttgaaaga aggcgcaatc gccgccgggc ttacggcact gatgattctg
      181 ctgtttctcg gctcgtggcg cagcaccttg atcgttgccg tctcgatacc gctgtcgatc
      241 ctcaccggga tcatcatcct gtggggcatg ggtcagtctc tcaacacaat gactctgggg
      301 ggcatggcct tggcggtcgg cattctggtc gacgacgcaa cggtcgagat cgaaaacgtg
      361 caccgcaacc tcggcatgag aaagccgatc cgccgcgcga tcttggacgg ggccgcgcaa
      421 atcgcggtgc ccgcgttcgt gtcgaccttg gccatctgca tcgtcttcgt gccggttgtg
      481 ttcctcacgg gtccggccgc ctcgctcttt accccgctgg ctctctccgt cgttttcgcg
      541 atgatgacct cttatttcct ctcccgaacc ctggtcccaa ccatggtact ctacctcctt
      601 ccgcccgagg tcgagctcta cgccgaaggc gagcacggtg gatcgggcgg agggccgatc
      661 tggcgattcc accgggcctt caaccggcaa ttcgagcgcc tgcggcggtc gtatgagcga
      721 tttcttagct gggcgctcgc gcaccgcgcc gtgaccttga ccggcttgct tggcttcgcg
      781 ctgggctcgc tcgcgttaac cccgttcatc ggtcgagatt tcttcccggc ggtcgatgcc
      841 ggccagtttc ggctccatgt ccgggccccg gctggcacgc gcatcgagga aaccgaacgg
      901 atcttcggtc aggtcgagga agtgatccgc gaaattgtgc cggcccacga acgggagttg
      961 attttggaca acatggggat gtccccgtcg tttacggtcc gcgcctacat cgacaacggc
     1021 acagtcagcg acgccgacgg cgagatcctg gtgtcgctca aggaggaaca cgggccgacc
     1081 gcgggctacg tcgccaagct gcgccgagaa ttgccgcgac gtttcccgga ctgtatgttc
     1141 ttctttcagc ccgccgatat caccagccag atcctaaatt tcggcttacc cgcaccgatc
     1201 aacgtccagg tggtcggcgt caacgccgcc gggaacctgg ccgccgccaa gaaactgcgc
     1261 cgggaggtct cgaagattcg cggcatcgcc gacgttcacc tccaccagat caccgatcag
     1321 ccgaccctgc ggctggacgt cgaccggatc atggcctcgg agctcgggct ctatcagcaa
     1381 aacgtcgccg gcagcgtgct ggtgtcgctc agctcgacca gccagacgac accgaactac
     1441 tgggtcaacc cgcttaaccg ggtcaattac gtactggctg tgcagacccc gccggatcgt
     1501 atggattcgg tcgacgcgat gatgaacacg ccgattctca actaccaggc cgggc
//
LOCUS       DATAQL010001267         4659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_281549, whole genome shotgun sequence.
ACCESSION   DATAQL010001267 DATAQL010000000
VERSION     DATAQL010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281549"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..3335)
                     /locus_tag="VHS97_21830"
     CDS             complement(222..3335)
                     /locus_tag="VHS97_21830"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX3450910.1"
                     /translation="MATVSEQAGIATASDKQGEAARDGGRDRGRGRRGMVVPRVFSVE
                     GTNPFDQVEWDLRAAEIKDERGRAIFQQLECEVPRNWSQLATNVVASKYFYGDLASGN
                     GSPAEGKREFSVRQLVDRVTRTIADWGKADGYFATAEDAGRFHDELTSLCLNQYGSFN
                     SPVWFNVGLFHEYGISGPANNWRWDEETRSVVKATSAYQFPQASACFIQSVSDDMEGI
                     MGLAKSEAMLFKFGSGTGTDLSTLRSSQEKLSGGGKPSGPVSFMRVYDAIASVVKSGG
                     KTRRAAKMQTLKCWHPDILEFIECKTKEEKKAQSLIRAGYEANFNGEAYSSVMFQNAN
                     LSVRCTDSLLSAVESDGDWTTRSVTTGRPMATFKARLILDKIALGTWLCGDPGIQYED
                     TIQRWHTCPNTAPINSSNPCSEYMFIDDSACNLSSINLMKFRREDGSFDVERFRAAVR
                     IFITAQEILVDHASYPTDRIAANSHKFRPLGLGYANLGSLVMASGLPYDSRDGRALAA
                     AITAIMHGQAYLTSSEHAAHVGAFDGFAVNREPMLRVMEMHRDAAEAIDGSAAPELLT
                     HARAVWSECLDMGRKHGYRNSQVTVLAPIGTIAFMMDCDTTGIEPDIALVKYKQLAGG
                     GMLKIVNRTVPMALRKLGYDEPQIRGVLDYIDAHDTIEGAPEMKDEHLPVFDCAFGPP
                     QGGRSIHFRGHLRMMAAVQPFLSGAISKTCNVPNEATVEEIRGAYLEGWKLGLKALAI
                     YRDGSKGSQPVSTKSESSDEAGAPGAEVAAAASGAPIPAQAVNLAKGASDETRSSVSV
                     EPAPAPANQPRRERLPHTRRSLTHKFDIQGHEGYINVGFYPDGRPGELFITMAKEGST
                     IGGLMDVLGTAISIGLQYGVPLEVFVNKFAHSRFEPAGFTKNPDIPIAKSIADYIFRW
                     LGMEFIPGYRETNAPKRPSSEIEVEPASARAPMLKVNGHRTATIADLEHAEAVMGAGA
                     YQAEPVAAPAAVSHETVLGDGLGEQERQFAHFQSDAPACDNCGALTVRCGTCYRCFNC
                     GNSMGCS"
     gene            complement(3845..4162)
                     /gene="rplU"
                     /locus_tag="VHS97_21835"
     CDS             complement(3845..4162)
                     /gene="rplU"
                     /locus_tag="VHS97_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX3450911.1"
                     /translation="MYAIIEEGSHQFRVREGDRIRVDRRDGKAGDELVFSKVLLVTGE
                     NGNPTIGTPAVAGARVVGKIVNQFRAKKIIIQKFRRRKNVRRRNGHRQPFTTVQITSV
                     VPA"
BASE COUNT          889 a         1473 c         1345 g          952 t
ORIGIN      
        1 aaccttgcgc aaggcaatca attgttctaa cgagcatagt tgtccgcgtt tgtagggtgg
       61 gcaccgccca ccggtccgct gtcggtgggc gatgcccacc ttacaaactc gaaatacggt
      121 aaatcgactt gtcccgcaat gaaaaaccct cacccggcat tgtgccaggt gagggttttt
      181 cctttggtag ccagcagtgc gacccgactc gcaattccgc gttacgagca tcccatgcta
      241 ttgccgcagt tgaagcagcg atagcatgtg ccgcaacgga cggtgagcgc gccgcagttg
      301 tcacaagccg gggcatcgct ctgaaaatgg gcgaactgac gttcctgctc gccgaggcca
      361 tcacccagca ccgtctcatg actcaccgcg gccggtgcgg caactggctc ggcctggtag
      421 gcccctgctc ccatcacggc ctcggcatgc tcgagatcgg cgatcgttgc ggtgcggtgg
      481 ccgttaacct tgagcatggg cgccctagcc gaggcgggct cgacctcgat ctccgaactg
      541 ggccgcttgg gagcgttggt ttcacggtag cccgggataa actccatgcc cagccagcgg
      601 aagatgtagt cggcgatact cttggcgatc gggatgtccg gattcttggt gaatccggcc
      661 ggctcgaacc ggctgtgggc gaatttgttg acaaagacct ccagcggcac gccgtattgc
      721 agtccgatcg agatcgccgt gccgagcaca tccatcaggc cgccgatggt cgaaccctcc
      781 ttggccatgg taatgaaaag ctcgccaggc cggccgtcgg gataaaaccc cacgttgatg
      841 tagccttcat gaccctgaat gtcgaactta tgggtcaggc tgcggcgcgt gtgcggcagc
      901 cgctcgcggc ggggttgatt ggccggcgcg ggagccggtt cgaccgagac cgacgaccga
      961 gtctcgtcgc tggcgccttt cgccaggttg accgcctgag ccggtattgg cgcgcctgag
     1021 gctgccgcgg caacttcagc gcctggggcg cccgcctcgt cgcttgactc ggacttcgtg
     1081 ctcacgggct ggctcccctt cgatccgtcg cgatagatag ccagggcctt gagccccagc
     1141 ttccaaccct ccagatacgc gccgcggatc tcttcgacgg tagcctcatt cggcacgttg
     1201 caggtcttgg agatcgcgcc ggacagaaac ggctgaacgg cggccatcat ccgcagatgc
     1261 ccacggaaat ggatgctgcg ccctccctgg ggcggcccga atgcacagtc gaataccggc
     1321 aggtgctcgt ctttcatctc gggcgcaccc tcgatcgtat cgtgggcgtc gatgtagtcg
     1381 agaaccccgc gaatctgcgg ctcgtcgtag ccgagcttgc gcagtgccat cggcacggtg
     1441 cggttgacga tcttgagcat gccgccgccg gccagttgct tgtatttcac cagtgcgata
     1501 tcgggctcga ttcccgtcgt atcgcagtcc atcatgaaag cgatcgtgcc gatcggggcc
     1561 aagaccgtga cctggctgtt gcgatagccg tgcttgcgcc ccatgtcgag acactccgac
     1621 cacacggccc gcgcgtgagt caaaagctct ggcgccgccg agccgtcgat cgcctccgcc
     1681 gcgtcgcgat gcatttccat gacccgcagc atcggctctc tgttgaccgc gaagccgtcg
     1741 aacgccccga cgtgggccgc gtgttcggaa ctcgtcagat acgcctgacc gtgcatgatc
     1801 gccgtgatcg cggcggcgag cgcccgtccg tctcgtgaat catagggaag cccggaagcc
     1861 atcaccaggc tccccagatt ggcatagccc agccccagcg gccggaactt gtggctgttg
     1921 gccgcaatcc ggtcggtcgg atagctggcg tggtcgacca gaatctcttg cgccgtgatg
     1981 aagatccgca cggccgcgcg aaaccgttcg acatcaaagc tgccgtcttc gcgccggaac
     2041 ttcatcaggt tgatcgacga caagttgcag gcagaatcgt cgatgaacat gtattcgctg
     2101 cacggattgg atgagttgat cggcgcggta ttggggcaag tatgccagcg ctggatcgta
     2161 tcttcgtact ggatgccggg gtcgccgcag agccaggtcc ccagggcgat cttgtccaag
     2221 atcagccgcg ccttgaacgt cgccatcggc cgccccgtcg tgaccgaacg ggtggtccag
     2281 tcgccgtcgg actcgaccgc gctcaagaga gaatcggtgc aacgcaccga caggttggcg
     2341 ttctggaaca tgaccgagct atatgcttcg ccgttgaaat tggcctcata accggcgcgg
     2401 atcaatgact gggccttttt ttcttccttg gtcttgcact cgatgaactc gaggatatcc
     2461 gggtgccagc atttgagagt ctgcatcttc gccgcgcggc gcgtcttgcc acctgatttg
     2521 accacgctgg cgatggcgtc atagacccgc atgaaactga ccggccccga cggtttgccg
     2581 ccgccggaga gcttctcttg gctcgaacgc aaggtcgaca ggtcggtgcc agtccccgaa
     2641 ccaaacttga acagcatggc ctcgctcttc gccagcccca tgatcccttc catgtcgtcg
     2701 ctgacgcttt ggatgaagca cgctgaggct tgcggaaact ggtaggcgct ggtggctttc
     2761 acgacgctgc gcgtctcttc gtcccaccgc cagttgttcg ccggccctga aatgccatat
     2821 tcgtggaaca accccacgtt gaaccagacc ggcgagttga atgacccata ctgattgagg
     2881 cacaacgacg taagctcgtc gtggaatcga ccggcgtcct cggcggtggc gaaatagccg
     2941 tcagccttgc cccaatcggc aatcgtgcgc gtcacccggt caaccagctg gcgaaccgag
     3001 aattcacgct tcccctcggc cgggctgccg ttgccgctgg ctaagtctcc gtaaaagtac
     3061 ttgctggcca ccacgttcgt ggcgagctgg ctccagttcc gcgggacctc acactcgagc
     3121 tgctgaaaga tcgctcgccc acgttcatcc ttgatctcgg cggcgcgcag gtcccattcg
     3181 acctggtcga acgggtttgt cccttcgacg ctgaagacgc gcgggacgac cattccgcgc
     3241 cgccctcgcc cacgatcccg gccgccgtcc cgcgcggctt ccccctgctt gtccgacgcc
     3301 gtcgcaatcc ctgcttgctc gctcaccgtt gccattgacc gctctccatg tgtcgacgcg
     3361 ccctgggttg gccaacgaat ccgcaccacc caagctagcc gatttgtgtt caaaaatcca
     3421 taggtgcaca aaagtcgagt taagttttta agcctaactc acagtatcgg cagaccgggg
     3481 gaccgatttg aatctgtctc gacgagtgaa aatggaacgc gcggccacga aacccgccga
     3541 aatgccggcc ccgcgcccaa aatctgcaag cctcggacca gcgcggcgac aatgtgtgaa
     3601 tgacattccc agaattgcca gttcacaccg aaccgcatca ctcccccggc gatccatcgg
     3661 ttctctccta gtagcagcct cctgcgtccg gcctcgcagc gccgactatg gtaacatccg
     3721 attcccactt ggaaacaatc cacgcccgca aaataaaaaa accgctctct cgccgtaaaa
     3781 atcggcataa tagagcggtt taggcaatat tcggaatata caacgaccag caaaagacgg
     3841 gtagctacgc tgggacgacg ctggtgatct gcacggtcgt aaatggctga cgatgaccgt
     3901 tgcgccggcg cacgtttttg cgtcggcgga acttctggat gatgatcttc ttggccctga
     3961 attggttgac gatcttaccg accacacggg cgccggcaac cgcgggcgtc ccgatagtcg
     4021 ggttgccgtt ttcgcccgtg acaagcagga ctttcgagaa gaccagctcg tcgccggcct
     4081 taccatcgcg gcggtcaacc cggattcggt ccccttcacg aacccggaac tgatgactac
     4141 cttcctcaat gatcgcgtac atccgtcaaa tctccttagc cggggccggc cgcatcattt
     4201 gcagcggaat gaacagtttg tccgcgaaga ccaaccccgt gattcaagag cacttggctc
     4261 ttcgttttgt cgttcatgat catcgttggc tcgactggtc gagaacttaa catggtaaac
     4321 gccttgtgcc gaaaagagaa gggccggctt gaccgtcaaa cggattcgag atatcaagcg
     4381 ccaccactcg ttttttctgc cggtcggatc gactgaaaat agcccgtctt gcccgcccgt
     4441 tgaggcaact ttcgttagcc gtttgcatct gcagttggac ggacccgtcc aaccatctcg
     4501 ctcgaataag cggttctaaa gctggggact gcacgaagcc ttgtttcaat cacgcttgca
     4561 ctcattgatg ctgccgtgga cactgatggc cttgtttagc caggtggtga gtccacgcga
     4621 accgatggcg cagaaggatc gggctgcgga aactgtcga
//
LOCUS       DATAQL010001268         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2421815, whole genome shotgun sequence.
ACCESSION   DATAQL010001268 DATAQL010000000
VERSION     DATAQL010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2421815"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(600..>1522)
                     /locus_tag="VHS97_21840"
     CDS             complement(600..>1522)
                     /locus_tag="VHS97_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX3450912.1"
                     /translation="FRIFLEMRRSPWLDCDHLASSGQSIEGSVGDVRQVEYPDDSPTD
                     FAQDHFKEGMAAMKYRPLIGVNSDYRATAKGRVPHCYVHSGYFDCLLSANALPVFIPP
                     LIRESDLMPILDRLDGVMLTGGDDLDPKKMGLTPHLAVTVVPERRELADRLLCKLVQQ
                     RRIPVLGIGLGMQELNVCAGGGLFLHLPEDMPKSIPHRDPHGGAHRHIVVMQPGTRLE
                     EIYGPGEIRVNSYHHQGIRKLAPIFRVAALAPDGLIEGFEGKDHSWWVTGVQWHPENE
                     GNISLDMQLIEAFVSAAATSKSMPALAKVG"
BASE COUNT          310 a          430 c          472 g          310 t
ORIGIN      
        1 tgaaaccacg atcaaggccg tcgcggtgat gcgtttcgtg ccgttcatcg gtttcccttt
       61 ttttcgttga gctcgcgtat gctagtggaa accctgggca accaggcagg gcgaaccgat
      121 ccgtgggaaa tccgcgcgga cggcgaccgc ggctcgaaag agagtcgagc cacagagccg
      181 cgccccttcc gtggtcctgt ggaggcgcag ggatgtctta ctgaagaccg atctatttat
      241 ccgaggaatg gccaggaaga tccggcgcct cgggagttaa gaatcggcgg atccgccgat
      301 ggtgggctgg tagagcaagt cgcgcgccga accagttttc gatgaaaatt gagtggtcta
      361 gccgcagtcc gttcggcgac cgttcggttc gtatcgatcg tgcgggggcg caagcgaata
      421 gagagccggc ccgagtggtt gctttttaca agcgaggtcg ctgtaaaatc aacacacgag
      481 gcagctggct ccgttgtcac cgccagcggt cgttcgaaat gaaaaagcgg cgttcccagc
      541 agggtttccc caaataggaa cgccgccaac aacgaggcac cggggttggg ccgcagctgt
      601 cagccgacct tggcaagtgc tggcatgctc ttgcttgttg ccgcggccga aacgaatgct
      661 tcgatcagtt gcatatccag cgagatattt ccctcatttt ctgggtgcca ctgcacgccg
      721 gtgacccacc aggagtgatc cttgccctcg aatccctcga tcagaccgtc gggcgcgagc
      781 gccgccacgc ggaagatggg agcgagcttg cggatgcctt ggtggtgata ggaattgact
      841 cggatttcac cgggtccgta aatttcttcg agccgagtgc ccggctgcat gacgacgatg
      901 tgcctgtggg ctcccccatg ggggtcgcga tggggaattg acttgggcat gtcttccggg
      961 aggtggagga acagcccgcc tccagcacag acgttgagtt cctgcatgcc caaaccgatg
     1021 cccaacacgg gaattctgcg ctgctggacg agcttgcaaa gtaagcggtc cgccaactcg
     1081 cggcgctcgg gcacgacagt gaccgccaga tgcggtgtca agcccatttt tttcggatcc
     1141 aggtcatcgc cgccggtcag catcacgcca tccagccggt cgagaatcgg catgaggtcg
     1201 gattccctga tcaagggcgg tatgaatacc ggcagcgcgt ttgcggacag caagcaatcg
     1261 aagtagccgc tgtggacgta acaatggggc acgcgaccct tggccgtcgc ccgatagtcg
     1321 ctgttgaccc cgatgagcgg acggtatttc atggcagcca tcccttcctt gaaatggtct
     1381 tgagcgaaat ccgttggtga atcgtccgga tactctactt gccgaacgtc gccgactgat
     1441 ccttcaatcg actggcctga actggccagg tgatcgcagt ctaaccacgg gcttcggcgc
     1501 atttcaagga aaatccggaa aa
//
LOCUS       DATAQL010001269         7512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4556114, whole genome shotgun sequence.
ACCESSION   DATAQL010001269 DATAQL010000000
VERSION     DATAQL010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4556114"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VHS97_21845"
     CDS             <1..943
                     /locus_tag="VHS97_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX3450913.1"
                     /translation="NRLGLARWLVDSANPLTARVAVNRFWAQIFAAGLVETEEDFGTQ
                     GEPPSHPELLDWLAIRYQESGWNTKALLRLIVTSATYRESSRVSPELLEKDPRNRLLG
                     RASRVRLEAEMVRDQALALSGLLSQRIGGPSVFPAQPEGLWQAAFNGQRTWSTSAGED
                     RYRRGLYTFWRRTVPYPSMAVFDAPSREICAIRRIRTNTPLQSFVTLNDPVYVEAAQS
                     LARRIIRDGGINVQSRVRYGLQLCLCRPPRPEQIEPLIGLYTTEYKRYCKDTPAALAL
                     ATVPLGPLPPCMEPADLAAWTAVANVLLNLDGVLTKG"
     gene            949..2454
                     /locus_tag="VHS97_21850"
     CDS             949..2454
                     /locus_tag="VHS97_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450914.1"
                     /translation="MDLPWKLHLARAQTRRQFLKTSQAGLGAIALRSLMAGSGAAWGK
                     EPEARGGKDRALLGRSSDNPMTPRPPHFAPKATRVIYLHMSGGPPQQDLFEFKPELVK
                     HHLQPCPDELLKNQKFAFIKGHPKLLGSPYRFQKWGQSGAMVSELLPHVAGAVDDIAI
                     IKSMYTDQFNHAPAELFLYTGSSRNGGAAMGSWITYGLGSENQDLPGFVVLISGGTDP
                     TGGKALWSTGFLPSVYQGVQCRTVGDPILYVSDPKGMDRTDRRHTLDALKQLNEYELA
                     EFGDPETLTRISQYELAFRMQMSVPDVMDIRQEPEQILAEYGARPGASSFANNCLLAR
                     RLVERGVRYVQLFDWGWDCHGTGTGDDIVTHLPEKCKEVDRPIATLLADLKRRGLLDE
                     TLVIWGGEFGRTSMNEARGGSKYLGRDHHPNCFTIWMAGGGIKPGITVGATDDLGYTI
                     TDNPVSIHDLQATILHLLGLDAWKLTYAYQGLNQKLIGPEGEAKIRKELLA"
     gene            complement(2565..4667)
                     /locus_tag="VHS97_21855"
     CDS             complement(2565..4667)
                     /locus_tag="VHS97_21855"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX3450915.1"
                     /translation="MAAASTPYVGCPSSRFAKADHHLSRFHMNQYGFVRITCASLRTA
                     VANPAKNADEILRVLDLATDSDIVVCPELSVTSYTCADLFGQSTLIDAGIRETLRIAR
                     ATVGRAQLVVVGAPIPVGNSLFNCAIVIGDGSILGIVPKQYIPNYKEFYESRWFSPAA
                     AGTVTYIELDGLRIPFGTDLLFEAKTHERSPGNGSVIVGIEICEDLWVPIPPSAFQAM
                     AGATVLLNPSASNETIGKSRYRTDLVVGQSGRTISAYAMAGSGPSESTTDVVFGGHCL
                     IAENGRLLAESPRVGDGQPVRRDSYTITQDVDIAKLETDRKVTTTFDDGSSTVKPFRR
                     IPFSLAETMEGLKRDVPGTPFVPMEGPELHRRCAEIFGIQCAGLAKRIEQLPPRTPLY
                     LGVSGGLDSTLALLVSVSTCDSLGLDRTLIHGITMPGFGTTQRTRTNALALMEHLGVR
                     AETIDISALALQAFEEMGHAPFGIECRGLDVASFRAALARVPREACQDLTFENVQARL
                     RTFLLMSKGFVVGTGDLSELALGWSTYNGDHMSMYNPNCSIPKTLVKFLVRYVALNQF
                     PEGAVRQTLLSIVETTISPELLPVSTAGEIEQSTEATLGPYELLDFILYHAIRCGYSP
                     AKILFMSQFATFTQTYPRGLIERTLGTFFSRFFRQQYKRSCVPDGPKVGTVSLSPRGD
                     WRMPSDADPSEWLKWGQD"
     gene            4807..>7512
                     /locus_tag="VHS97_21860"
     CDS             4807..>7512
                     /locus_tag="VHS97_21860"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX3450916.1"
                     /translation="MSASLLQRRLSSDSPLEFRSQHSMPAFNRAVGVWLIVAAAATYP
                     GALAQSSGAGRTAPERRPFAKTGTPRRTERIRFFDVKHIKAELLIDTKKRELRGTVTH
                     SLSPLHPYLKQLELDCGPQLKVSRVTVGPKRNRCTFATKDGKLAVTLDKAYGLGDTLE
                     LAIEYTGMPDRGLYFVMPETPYPEKSLSVWTQGEAEDTHHWLPCYDYPNERATSEMII
                     TVEKPLFVLSNGALVETKEHDGNTTTYHWKMNVPHSSYLISLAASDFAIYHDRAGDLP
                     VDYYVAKHVDEATARRFMGHTPRMIRFYGEKLGQPYPYAKYAQVCVPDFIAGGMENIS
                     ATTMTDSALHDEIAELEHQEDGLVAHELAHQWFGDLLTCKDWSHLWLNEGFASYFDPL
                     YAQHDLGDDVFRLEMKGSQDGYLGGDREYRRPIVESRYETSDDMFDGVTYSKGACVLH
                     ALRGLVGEEAWWRGIRGYVSAHKFDVVETDDLRTAMESAYGKDLKWFFDQWVYKAGHP
                     ELKVRWHYEDADKTVRVRFQQTQTLDDQTPLFRLPTTLEITEDVGKTRAIPIVIDGAS
                     HEFVIPAAARPKMVQIDPEGWLIKELDFEKDVEENLFQLEHAACILGRLDAARALVKP
                     AKDKPEVAKALAAAWKREKTARGQREMVEILANGDDVFRSALMDAAKASDARPRVSAI
                     AGLSKLSRDDAAEEALRAAWKNPTEAYGARQAALRGLAAWKVKDADQLVQGALKTSVD
                     HHSLAAAALEILLQTPGAKARELAALYSKYGQPHTMRSSAIKAFPRLAKDDPTLQDVI
                     VELADDNDRSVRFRAWDAVKELRIRKALPVLEARLAKDIGDFGGSARRVLEDTISLLK
                     EQPAEAKSDSGPTAEQAKTIAELERQAADLELKSKELRTKIAALKA"
BASE COUNT         1516 a         2386 c         2252 g         1358 t
ORIGIN      
        1 aaatcggctc gggctggcgc ggtggctcgt cgattcggcg aaccctctga cagcgcgtgt
       61 ggccgtgaat cgcttttggg ctcagatctt cgccgcggga ctcgtcgaaa ccgaagaaga
      121 tttcggcacg cagggtgagc cgccgagcca tcccgagcta ctcgactggc tcgccatccg
      181 ctaccaagaa tcaggctgga ataccaaggc gcttctgcgg ctgatcgtca cttcggccac
      241 gtatcgcgag tcttcacggg tcagccccga gcttctcgag aaggatcccc gcaaccggct
      301 cttgggacgg gcgtcccgag tccggctgga ggccgaaatg gttcgcgacc aggcgctggc
      361 gttaagtgga cttttgagcc agaggatcgg tggacccagc gtattccccg cgcagcccga
      421 gggcctgtgg caggcggcgt ttaacggcca gcggacctgg tcgacgagcg ccggtgaaga
      481 ccgttatcgc cgcggcctct ataccttctg gcgtcgaacc gttccctatc cctcaatggc
      541 cgtctttgac gctcccagcc gcgaaatctg cgcgattcgc cgcattcgga ccaacacccc
      601 gctccagtcg ttcgtcacgt tgaacgaccc ggtatatgtc gaggccgccc agtcattggc
      661 gcgcaggatc attcgcgacg gtggtataaa cgtgcaatca cgcgtccgct acggcctgca
      721 gctctgcctt tgccggccac cgaggcccga acagattgaa ccattgattg gcctctacac
      781 tacggaatac aaacgatact gcaaggatac gcccgccgcc ctggccctgg ccaccgtgcc
      841 gctcgggccg ctacctccgt gcatggagcc ggctgatctg gctgcatgga cggccgtcgc
      901 caacgtactc ttgaatctcg atggcgtctt gaccaaaggc tgagtttcat ggatctgccc
      961 tggaagttgc acctcgcgcg ggcgcagacc cgccggcagt ttttgaagac gtcgcaggcc
     1021 ggcttgggcg ccatcgccct caggtcgctc atggcgggca gtggtgccgc ttggggaaaa
     1081 gagcccgaag cccgcggtgg gaaagaccgc gcccttctag gtcgctcgag cgacaacccc
     1141 atgacccccc ggcccccaca cttcgcgccc aaggccacgc gggtgattta tctgcatatg
     1201 tcgggcggac ctccgcagca ggacctgttt gaattcaagc cggaactggt caagcaccac
     1261 cttcagcctt gccctgacga attgctcaag aatcagaagt ttgccttcat caaaggccac
     1321 cccaagctgc tcggttcgcc ctatcgtttt cagaaatggg gacagagcgg cgcaatggtg
     1381 agcgaactct tgccccacgt ggccggcgcg gtcgacgaca tcgcgatcat caagtcgatg
     1441 tataccgacc agtttaacca tgcgccggca gagctgtttc tctacaccgg ctcgtcgcga
     1501 aacggcggtg ccgccatggg ctcctggatc acctacgggc tgggttcgga aaaccaagac
     1561 ctgcctggat tcgtcgtctt gatcagcggc gggaccgacc ccaccggcgg caaggccctc
     1621 tggagcaccg gctttctccc gagcgtgtat caaggcgtac agtgtcgcac cgtcggcgat
     1681 ccgattctct atgtcagcga ccccaaaggc atggaccgaa cggatcgacg ccacactctg
     1741 gacgcgctca agcagctcaa cgaatacgag ctcgccgagt ttggcgatcc cgaaacactt
     1801 acacgcatca gccagtacga gctggccttt cggatgcaga tgtccgttcc cgacgtcatg
     1861 gatatccgcc aggaacccga acagatcctg gcggaatacg gcgcgcgacc gggggcgtct
     1921 tcgtttgcca acaactgcct cctggcgcgc cgcctggtcg agcgcggcgt tcgctacgtc
     1981 cagctcttcg actggggctg ggactgccac ggcacgggca ccggcgacga catcgttacg
     2041 cacctgccgg aaaaatgcaa agaagtcgac cggccgatcg ccaccctttt ggcggacctc
     2101 aagcggcgcg gacttctcga cgaaacgctg gtgatctggg gaggcgagtt tggacgcacc
     2161 tccatgaacg aagcgcgtgg cggttcgaaa tatctaggac gcgaccacca tcccaactgt
     2221 ttcacgatct ggatggccgg cggcgggatc aagcctggca tcaccgtcgg cgccaccgac
     2281 gacctcggct acaccatcac cgacaatccg gtgagcattc acgacctgca ggccaccatt
     2341 cttcacctgc tggggctcga cgcctggaag ctcacgtacg cctaccaagg gttgaatcag
     2401 aaactcatcg gccccgaggg cgaggccaag atccgcaagg aactgctggc gtgaacgccc
     2461 gcgctgcgcc tctcgaatta attacggcca tttcgctcgt catgattggc accatcgccc
     2521 tgttactcgg ctgcgcacgc accgcaaacg tcgcgaccgc acgtttaatc ttggccccat
     2581 ttcaaccatt ctgacggatc ggcatcggaa ggcattcgcc agtccccgcg aggcgagagg
     2641 ctgaccgtgc cgaccttggg cccgtcgggc acgcaagacc gtttgtactg ctggcggaaa
     2701 aaccggctga agaacgtgcc gagcgtccgc tcgatcaatc cgcgaggata agtctgggtg
     2761 aaagtcgcaa actggctcat gaacaagatc ttggcgggtg agtacccgca gcggatcgcg
     2821 tgataaagga taaagtcgag cagttcgtag gggccgagcg tcgcctccgt cgactgttcg
     2881 atctcgccgg ctgtcgagac tggcaaaagt tccggcgaga tcgtcgtctc gacgatcgac
     2941 agcagcgtct gccgcacggc gccctccgga aattggttga gcgccacgta acggaccaaa
     3001 aacttaacca gcgtcttggg gatcgagcag ttgggattat acatgctcat gtgatcgccg
     3061 ttatacgtcg accagcccag cgccagctcg gaaaggtcgc cggtgccgac gacgaaccct
     3121 ttcgacatca gcaggaacgt tcgcagtctg gcctgcacgt tttcgaaggt gaggtcctgg
     3181 caggcttcac ggggcactct ggccagggcc gcgcggaacg atgcgacgtc aaggccccgg
     3241 cactcgatcc cgaacggagc gtggcccatt tcctcgaagg cttgtagcgc cagcgcgcta
     3301 atgtcgatcg tctccgctct aacgcccagg tgctccatga gtgccagggc attggtccgc
     3361 gtgcgctggg tcgtgccaaa tccgggcatc gtgatcccat ggatcaaggt gcggtccagg
     3421 ccgagactgt cgcacgtcga gaccgagacc aagagcgcga gcgtcgaatc cagccccccg
     3481 gagacgccga gatagagcgg agtcctggga ggaagctgct cgatgcgctt cgcgagcccc
     3541 gcacactgga tgccgaagat ctcggcacag cggcgatgga gctcagggcc ttccattggt
     3601 acaaacggcg tccccggaac gtctcgcttc aggccctcca tcgtttccgc cagcgaaaac
     3661 ggaattcgcc ggaagggttt taccgtcgac gagccgtcgt caaatgtggt cgtgaccttt
     3721 cgatcggtct ccaacttggc gatatcgaca tcctgggtga tcgtgtacga gtcgcggcgg
     3781 actggctggc cgtcccccac gcgaggcgac tcggccagga gccgtccgtt ttcggcgatc
     3841 aggcagtggc cgccgaaaac tacatcggtc gtcgattccg acggcccgct gcccgccatc
     3901 gcataggccg agatcgtgcg gcccgactgg cctacgacca gatcggtgcg gtaccggctc
     3961 ttgcctatcg tctcgttgct ggccgacggg ttcagtaaga cggtcgcgcc ggccattgct
     4021 tggaaagcac tcggaggtat cggtacccac agatcctcgc agatctcgat gcccacgatg
     4081 acgctgccat ttccgggtga gcgttcgtgc gtcttggcct cgaacaggag atcggtccca
     4141 aaagggatac gcagtccatc gagctcgatg tatgtaaccg taccagccgc ggccgggctg
     4201 aaccatcgac tctcgtagaa ttccttgtag ttgggtatgt attgcttggg aacaatcccc
     4261 aagatagagc catcgccgat cacgatcgcg cagttgaaca gactgttgcc gacgggaatc
     4321 ggtgccccga cgacgaccag ttgcgcgcgg cctacggtcg cccgggcgat ccgcagcgtc
     4381 tcgcggatgc cggcgtcgat cagcgtcgac tgtccaaaga ggtcggcgca ggtgtagctc
     4441 gtgacagaaa gctcggggca gacgacgatg tcactatcag tcgccaggtc caggacgcgc
     4501 aagatctcgt cggcgttctt tgccgggttg gccacggccg tccggagcga ggcacaggtg
     4561 attcgtacaa acccatactg gttcatgtga aatctactca aatggtggtc cgccttagcg
     4621 aaccgggacg aagggcagcc gacatagggc gtacttgcgg ccgccacgag ttgcctccca
     4681 cgacaatcga ctcgcggcga ggttgacccc gtcgtaacat cgcgagtaaa acataacatg
     4741 acgtacaaca gcctttgtcc ggctcaaaag agagcgcccc gtcctttctt tacggatcga
     4801 taccgcatgt cggcctcgct tcttcaacgt cgcttgagct cggattcgcc gctcgaattc
     4861 cgatcccagc atagcatgcc cgccttcaat cgcgccgtcg gagtgtggct cattgtagca
     4921 gcggctgcca cttacccggg cgccttggct cagagctctg gtgccggccg cacggcgccc
     4981 gagcggcggc cgtttgccaa gacgggtacc ccccgtcgca ccgagcgcat ccgcttcttt
     5041 gatgtcaagc atatcaaggc cgagctgttg atcgacacca agaaacgcga attgcgcggg
     5101 acggttaccc attcgctcag cccgctgcac ccctatttga agcagctcga gctcgactgc
     5161 ggaccccagc tcaaggtgtc cagagtgacc gttggtccca agaggaaccg ctgtactttc
     5221 gcaaccaagg acggcaagct ggccgtcacg ctcgacaaag cctatgggct cggcgacacc
     5281 cttgaactgg caatcgaata cacaggcatg cccgaccgcg gactctactt tgtcatgcca
     5341 gagacaccgt atccggaaaa aagtctgtcg gtctggactc agggtgaggc tgaggataca
     5401 caccactggc ttccctgtta tgactaccct aacgagcgcg ccaccagcga aatgatcatc
     5461 acggtcgaaa agcctctgtt tgtactgtcc aacggggcgc tcgtagaaac caaggaacac
     5521 gacggcaaca cgacaactta ccactggaag atgaacgtcc cacattccag ctacctgatc
     5581 tcgctggccg catccgattt cgccatatac cacgaccggg ccggagatct cccggtcgac
     5641 tattacgtgg ccaagcacgt tgacgaagcg acggcccgac ggttcatggg ccacactccg
     5701 cggatgatcc gtttttacgg cgaaaagctg ggtcaaccgt acccctacgc caaatatgcc
     5761 caggtgtgcg tgcccgactt catcgccggc ggcatggaga acatctccgc caccacgatg
     5821 accgactccg cgctccatga cgagatcgcc gaactcgagc atcaggagga cggcttggtg
     5881 gcccatgagc tcgcccacca gtggttcggc gatctcttga cctgcaagga ttggtcgcac
     5941 ctctggctca acgaggggtt tgcttcctat ttcgacccgc tctacgccca gcacgacctg
     6001 ggcgatgatg tattccggct cgagatgaaa ggatcgcagg acggctacct tggtggcgac
     6061 cgggaatatc gccgcccgat cgtcgagtct cgctatgaaa catccgacga catgttcgat
     6121 ggcgtgacct attccaaggg cgcctgcgta ttgcacgcgc tgcgcggctt ggtcggcgaa
     6181 gaggcgtggt ggcgggggat ccgtggctac gtgtccgcac acaagttcga tgtggtcgaa
     6241 accgacgacc tgcgcacggc gatggaatcg gcttatggta aagacctcaa gtggttcttc
     6301 gatcagtggg tttacaaggc cggccatccg gagctcaagg ttcgctggca ctatgaagac
     6361 gccgacaaga cggtacgtgt gcgattccag cagacgcaga cgctcgacga ccagactcct
     6421 cttttccgcc tgccgacgac actcgagatc acggaagacg tcggcaagac gcgcgctatc
     6481 ccgatcgtaa tcgacggggc ctcgcacgag tttgtgatcc cggcggcggc ccgacccaaa
     6541 atggtccaga tcgacccaga gggctggctg atcaaggagc tcgacttcga gaaagacgtc
     6601 gaggaaaacc tgtttcagct cgagcacgcg gcgtgcatat tgggccggct ggacgcggcg
     6661 cgggctctgg tgaagccggc gaaggataag cccgaagtcg ccaaggcgtt ggcggcggcc
     6721 tggaagcggg aaaaaacagc gcgcggccag cgcgagatgg tcgaaatcct tgcgaacggc
     6781 gacgacgtct tccgaagcgc gctcatggat gcggccaagg cttccgatgc ccgcccgcgc
     6841 gtatcggcga tcgccggcct gtcgaagctc agccgcgacg atgccgccga ggaggcgttg
     6901 cgcgccgcgt ggaagaatcc cacggaagcc tacggcgccc gccaggccgc actgcgcggg
     6961 ctggccgcat ggaaagtcaa agacgccgac cagttggtcc agggcgcttt gaaaacgagc
     7021 gtcgaccacc actcgctcgc cgccgccgct ctcgaaatcc tgctgcaaac gcccggtgct
     7081 aaagcgcgcg agctggccgc gctttacagc aagtatggtc agccccatac gatgcgatcc
     7141 tcggcgatca aggcgttccc tcgcctggcc aaggacgacc cgactctcca ggacgtcatt
     7201 gtggaactgg ccgacgacaa cgatcggtct gtgcgatttc gggcctggga cgcagtcaag
     7261 gagctgcgca tccgcaaggc gctgccggtc ctcgaggctc gactcgccaa agatatcggc
     7321 gatttcggag gctccgcgcg ccgcgttctc gaagacacga tcagtctgct caaggaacaa
     7381 ccggctgagg ccaaaagtga cagcgggccg acagccgagc aggccaagac catcgccgaa
     7441 ctcgagcgcc aggccgccga tctcgagctc aaaagcaagg agctgagaac caagatcgcc
     7501 gctctcaagg cc
//
LOCUS       DATAQL010001270         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1198676, whole genome shotgun sequence.
ACCESSION   DATAQL010001270 DATAQL010000000
VERSION     DATAQL010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198676"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VHS97_21865"
     CDS             <1..282
                     /locus_tag="VHS97_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450917.1"
                     /translation="RTAMHHFRGKLFQGDKLCLDPANAYVDYDSREVDETSKWSGYLL
                     VGSEKDVVPGGTYTLKLEDGRAGQLRIDTLTPDDSDKVRAIFVGTGSLG"
     gene            379..753
                     /locus_tag="VHS97_21870"
     CDS             379..753
                     /locus_tag="VHS97_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450918.1"
                     /translation="MATDKIEKRLADARGRLNRAIKALAPKHQGGEWEEYLAAKESVL
                     KLERERAAAKREEYAIPLDFPVQWDIGAPLPYVIQNDNKTFLTFYVRETDPDWEPIFR
                     MKVLDTTKLSGGSGDIMPNPAA"
     gene            1093..1734
                     /gene="ycaC"
                     /locus_tag="VHS97_21875"
     CDS             1093..1734
                     /gene="ycaC"
                     /locus_tag="VHS97_21875"
                     /inference="COORDINATES: protein motif:HMM:NF041461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HEX3450919.1"
                     /translation="MPPDTTTALRGRLSADQSAVLLIDHQAGLLSLVQDYSPDEFRNN
                     VLALADTAKHFRLPTILTTSFEEGPNGVLMPELKAAFPDAPFIPRPGQINAWDNEDFV
                     EAVRKTGRKQLIMAGVVTDVCVAFPALCALEEGYQVFVVVDASGTFNKSVRDAALIRM
                     AHAGAVMVNWFAVACELARDWRNDMEGLAGLLGGHLPAYQNLITSYRANTAKR"
     gene            1802..>2002
                     /locus_tag="VHS97_21880"
     CDS             1802..>2002
                     /locus_tag="VHS97_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3450920.1"
                     /translation="MTFDEPADLILHGGKLTTLDPANPSATAAALKGDRILTVGSDEQ
                     ALVHFRGPKTTVIDLGGRRAIPG"
BASE COUNT          390 a          641 c          630 g          341 t
ORIGIN      
        1 agaacggcga tgcatcactt ccggggaaag ctcttccagg gcgacaaact ctgtctcgat
       61 cccgcaaacg cctacgttga ctacgattct agggaggttg atgaaacctc aaagtggtct
      121 ggctatctct tagtcggatc ggagaaggac gtggtgcctg gaggcaccta cacgctaaag
      181 ctggaggacg ggcgggctgg tcagctccgg atcgacaccc tcaccccaga cgactcggac
      241 aaggttcgag cgattttcgt cgggacgggg tcactggggt aagcggacgt agcagcaagc
      301 gaccgatgcc ctcgggactc tcgatcagct atcgctaccc tcggctgcga taaaggaggt
      361 cggagcaatc taccggccat ggccaccgac aagatcgaaa aacgactcgc tgacgctcgg
      421 ggtaggctca accgagcgat caaggctctc gcgccgaagc atcaaggagg tgagtgggag
      481 gaatacctcg ccgccaagga gtccgtgttg aaactggaga gggaacgcgc cgccgccaag
      541 cgcgaggagt atgccatccc gttggacttt cccgtgcagt gggacatcgg tgcgccgctc
      601 ccctacgtta tccagaacga caacaaaacg tttctgacct tctacgtccg agagaccgac
      661 cctgattggg aaccgatttt ccggatgaag gtgctcgata ctacgaaact ttcggggggt
      721 tcgggcgaca ttatgcccaa tccggcggcc tgatcttggg gccggctttg tatcgtgtcg
      781 gggccgtcat cccgccaaga attgaaggcg gtggttcgcc ccgacccatg cagttaattc
      841 ctgaacttgc tccgagcagg atggtaaagc cttcctgcgc actcattgcg tcttccagcc
      901 gacgccgatg aatcgtcggc ggcctaaccg aggcttgcgt gccgaggaat tcgtctttgc
      961 cgaaacgaaa cgcggcggct attctagacg cgtgtcccgc cgtccgcata ggagagaggg
     1021 gagatggcgt gagggacgaa atctcaggcc gcgaggtcgg ccgaccggga aaccgaaccg
     1081 cgggagacga gcatgccccc tgatactacg acggccctgc gcgggcgcct ctcggccgac
     1141 cagtcggccg tcctcctgat cgaccaccag gccggcctcc tgtcgctggt ccaggattat
     1201 agccccgacg agttccgcaa caacgtactc gccctggccg acaccgcgaa gcacttccgg
     1261 ctgcctacga tcctgaccac cagcttcgag gaaggcccta acggagtcct catgccggag
     1321 ctgaaggcgg ccttcccgga tgccccattc atcccgcgcc ccggccagat caacgcctgg
     1381 gacaacgagg acttcgtcga ggcggtccgg aagacgggcc gcaagcagct gatcatggcc
     1441 ggcgtggtca ccgacgtctg cgtcgcgttc ccggccctct gcgccctgga agagggctac
     1501 caggttttcg tcgtcgtgga cgcctcgggc accttcaaca agtccgtccg cgatgccgcg
     1561 ctgattcgga tggcccacgc cggggccgtc atggtcaact ggttcgcggt ggcttgcgag
     1621 ctggctcggg actggcgcaa cgacatggag gggctcgcgg gcctgctggg cggccatctg
     1681 ccggcctatc aaaacctcat cacgagctac cgcgccaaca ccgcgaagcg gtgacggggc
     1741 attgcccacc tcctgtccgc gaccggggga catcccggac aatgcaaggg agcgatcggc
     1801 catgactttc gacgagcccg ccgacttgat cctgcacggc ggtaagctca cgaccctgga
     1861 cccggccaac ccgtcggcga ccgcggcggc ccttaagggc gaccgcatcc tgacggtcgg
     1921 ctcggacgag caggccctgg tgcacttccg gggtccaaag acgacggtca tcgacctcgg
     1981 cggacggcgg gccattcccg gc
//
LOCUS       DATAQL010001271         4511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5472376, whole genome shotgun sequence.
ACCESSION   DATAQL010001271 DATAQL010000000
VERSION     DATAQL010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5472376"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VHS97_21885"
     CDS             complement(<1..725)
                     /locus_tag="VHS97_21885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3450921.1"
                     /translation="MEAQDHAGSDVRSAARRVVHAGLAIMETSDQVCIPVMKRISEIQ
                     KIAFVGDYLPRKCGIATFTYDLCTSVATQYPGSDCFVVPINDISSGYDYPAEARFEIE
                     EQEVDSYLRAADFLNFANTDIVCLQHEYGIYGGPAGSHVVRLLRDLRMPIVTTLHTVL
                     RDPTSEQHRILTQIADLSARLIVMSERARGFLRDVYDVPEAKIDLIAHGIPDMPFVDP
                     NFYKDQFAVEGKFVALTFGLLSP"
     gene            complement(1111..1593)
                     /locus_tag="VHS97_21890"
     CDS             complement(1111..1593)
                     /locus_tag="VHS97_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450922.1"
                     /translation="MKSYQQLSLFFVLAVLANGAAAQAQSQTDGRFNRVTTTGGRRAT
                     TNTSSQVRSVARSAGRTGAVGGPSARTDSLRPHIEAQARIQGQSVGTPQSSTWRQVPE
                     PPVAPPVVTESRSHNYYPGMRPSLAIQQPVTMTARATGIPHICTPSRSMMVGAGRHGR
                     "
     gene            1933..3309
                     /locus_tag="VHS97_21895"
     CDS             1933..3309
                     /locus_tag="VHS97_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3450923.1"
                     /translation="MSIITDRRSFLFGASVAGFGIFAQGRRGWARGVGPNETLNIACI
                     GVGGKGKSDTGHAARQGRIVALCDIDQIRLDEMAANHKEAKKYNDFRELLHELDSRID
                     AVVVSTPDHTHAAAAVTAMRLGKHVYCQKPLAHSVWEARLMRETARQKGVCTQMGNQG
                     TADPGFRRGAELVRSGAIGEVREVHIWTNRPFKYWKQSPDIVARPKETPPVPKHVHWD
                     LFLGPAPERPYNPVYHPHNWRGWWDFGTGSLGDMACHTANLTFMALQLGLPVRVSAQS
                     GEINSETYPAWATITYEFPARGDQPPVKVTWYEGAKDGKRNLPSGKLFPDSGFQPSDS
                     GSLLIGSKWRMYSPNDYGAVQMLWPAGEYKDLKIPERMLPRIEGAHGSDDGQKLEWVQ
                     AIRAGKPSLALSNFDYASNLTESMLLGNVAVRSGQAIDYNPETGEITGSSSASQYLKP
                     YFRKGWEI"
     gene            3313..4098
                     /locus_tag="VHS97_21900"
     CDS             3313..4098
                     /locus_tag="VHS97_21900"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX3450924.1"
                     /translation="MPTVEEVYHQAWGKVGGVGRLRRTFSLFVFMRQMLELQVKNKNP
                     GISGAELTWRTAKRMYFSSATTRQLLDRVGITSMATDDFPETIERILPILNELGVRFH
                     LTGGIAASYYGDPRFTQDLDVVIDLVNDRPETSLLLNRLSSGYFIDLPAAIDAIEHRS
                     LFQAIDERSMVKIDFHVGEKISGELDRATRREVVPGIMAPLVSKEDAILSKLLWIQQG
                     SGKSRHDVIEMLKRDEDLDRGCLRDRAATLGLSELLAEFENEI"
     gene            4171..>4511
                     /locus_tag="VHS97_21905"
     CDS             4171..>4511
                     /locus_tag="VHS97_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450925.1"
                     /translation="MPVSVLWGQTLFAASLLCWTFNLAHGDEPVKPTAPASETNPAVP
                     APGHSVHGEAFNDGPRHAAYRMPGMGKLHFPVATKQPEAQAFIDQGVAQLHSFYYFES
                     ERSFRQAAKIDP"
BASE COUNT          929 a         1374 c         1334 g          874 t
ORIGIN      
        1 ttgggcgaga gcagcccgaa cgtgagcgcc acaaatttgc cctcgaccgc gaactggtct
       61 ttataaaagt taggatcgac aaagggcatg tcggggatgc catgggcgat caggtcaatc
      121 tttgcttccg gcacgtcgta cacgtcccgc agaaagccgc gagcgcgctc cgacatcacg
      181 atcagccggg cggacaggtc ggcgatctga gtcaatatcc gatgctgttc gctggtggga
      241 tcgcgaagca ccgtgtggag cgtggtgaca atcggcatgc gcaggtcgcg cagcaggcga
      301 accacgtggc tgccggccgg gccgccgtaa atgccatatt cgtgctgcag gcagacgatg
      361 tcggtattgg cgaaattgag aaagtcggcg gcccgcaggt acgagtcgac ctcttgctcc
      421 tcgatttcaa accgagcttc cgcggggtag tcgtagcccg acgaaatgtc gttgatcgga
      481 accacgaagc aatccgaccc aggatactgg gtcgcgaccg acgtgcagag gtcgtatgta
      541 aaggtcgcaa tgccgcactt cctgggtagg tagtcaccga caaaggcgat cttttgaatc
      601 tcgctgatcc gtttcatgac gggaatgcaa acctggtcgc tcgtctccat aattgccaag
      661 ccggcatgaa cgacccggcg tgccgccgat cgaacgtcag agccggcgtg atcttgcgct
      721 tccattctac caagccttga ttccgccatt tccccgacag aacggagcaa aaccggtcac
      781 aaggaagtca gatccgtcca atgcacctgc ccgcgagcgt gggacgatgg tcgctggcat
      841 acccatcgtg ggtggccaat gcccctcggg cggaccttcg cccctatcat atcaagcctg
      901 ggcggacaaa caatccatgc gttgttcaag gcgtgctgcg ttccctagag cacttcccat
      961 tccgcccgat gcgccgtggc gcggcgtctg acgagcagtc tgcgcgaatg atccggcaac
     1021 tcgactccag ccacccagct tttgtcacct gcgaaggtgc tgaagttgaa ccatgccaaa
     1081 tagtgccagt cggtttttga cgtaactcta tcaccggcca tgacggccgg cgccaaccat
     1141 catcgatcgg ctgggagtgc agatatgcgg gataccggtg gcgcgggcgg tcatcgtgac
     1201 cggctgctgg atggcgagcg aaggccgcat ccccggataa taattgtgag acctagattc
     1261 tgtgacgacc ggcggagcaa ccgggggctc tggaacttgc cgccatgtcg acgactgcgg
     1321 cgtcccgacg ctctgaccct gaatccgcgc ctgagcctcg atgtggggcc gcagcgaatc
     1381 ggtccgggct gaaggtccgc caaccgcacc agtcctccct gccgaccgcg cgaccgatct
     1441 aacctgcgat gacgtgttgg ttgttgctcg ccgaccgccc gtcgtggtaa ctcgattgaa
     1501 gcgaccgtct gtttgactct gagcctgcgc cgcggcgccg ttggccaaga ccgccaggac
     1561 gaaaaacagg gacaattgtt ggtagctttt catcgaggta ctcggcaggt ttcggctcgt
     1621 cgagcgtacc acggccggat caactctgtc ctcttgaata tgcttcccct ttattatctg
     1681 cgcaacccgg cccaacacaa gagcccaccc aacctgaagc gtgggcaagg gtggatgatc
     1741 gccgccgagc gcatcggaat acactcgcaa ttggaccggg attgggacaa aatgaagctc
     1801 tgcgaggccg atatcgcggc cattgatcgt ttgtgtcgtt cctgcctttt gcttcgttgc
     1861 ctaccttgtg ccgtccgtcg tctccggtgt cgcggacgaa gagccttgag atcactccct
     1921 ggagccatgc tcatgagcat cattactgac cgtcggtcat tcctgtttgg ggcgtcggtt
     1981 gcgggtttcg gcatctttgc ccaaggccgc cgcggctggg cccgcggtgt cggaccgaac
     2041 gagaccctga atattgcctg tatcggcgtc ggcggcaagg gaaagagcga cacgggacat
     2101 gccgcacgcc agggccggat cgtggccctt tgcgacatcg accagatccg attggacgag
     2161 atggccgcaa atcacaaaga agccaagaaa tacaacgatt tccgcgaact gctccatgag
     2221 cttgattctc ggatcgatgc cgttgtggtg tcgactcccg accatacgca cgcggcggcg
     2281 gctgtcacgg ccatgcggct gggcaagcac gtgtattgcc agaagccact ggcccattcc
     2341 gtctgggaag cccgtttgat gcgtgagacc gcgcgtcaga agggggtgtg cacgcagatg
     2401 ggcaaccaag ggacggccga cccgggcttc cgccggggtg ccgagctggt gcgctccggt
     2461 gccatcggcg aggtgcgcga ggttcacatc tggaccaatc gcccattcaa atattggaaa
     2521 caatcgcccg atatcgtcgc ccgccccaag gaaacaccac cggtccccaa gcacgtccac
     2581 tgggaccttt tcctggggcc ggcccccgag cgcccgtaca acccggtcta tcatcctcac
     2641 aactggcggg gttggtggga ctttggaacc ggctcgctgg gagacatggc ctgccatacg
     2701 gcgaacctga ccttcatggc gctgcagctt ggtctaccag tgcgcgtctc ggcccagagc
     2761 ggcgagatca actccgaaac ctatcctgcg tgggccacga tcacgtacga attcccggct
     2821 cgcggcgacc agcctcccgt caaggtcacc tggtacgagg gggcgaagga cggcaaacgc
     2881 aatctgccca gtggcaagct ttttcccgac tcgggtttcc agccgtccga cagcggctcg
     2941 ctcttgatcg gttccaagtg gcgaatgtat tcacccaacg actacggtgc cgtccagatg
     3001 ctctggccgg cgggtgagta caaagatctc aaaatccccg agcgtatgct gccgcgcatc
     3061 gaaggcgctc atggttcgga cgacggacaa aagctggaat gggttcaggc catccgcgcg
     3121 ggcaagcctt cgctggcact atccaacttc gactacgcat caaacttgac cgagtcgatg
     3181 ctgctgggca acgtcgcggt gcgatcgggc caggccatcg actacaaccc tgaaaccggc
     3241 gagatcaccg gcagctcgtc ggcgtcacaa tatctcaagc cctacttccg caagggctgg
     3301 gagatctgag cagtgcccac ggtcgaagag gtgtatcacc aggcttgggg aaaggtcggc
     3361 ggagtcggca ggctccgccg gaccttttcg ctgtttgtgt tcatgcgaca aatgctcgaa
     3421 cttcaggtca agaacaagaa tccgggtatt agcggagccg aactcacctg gcgaacagcc
     3481 aagagaatgt atttctcgag cgcgacgacc cgccaactgc tggaccgggt gggaataaca
     3541 agcatggcga ccgatgactt cccggaaaca atcgaacgga tcctcccgat attgaatgag
     3601 cttggagtta ggttccactt aacaggaggg atcgcggcgt cttattacgg cgacccccga
     3661 ttcacgcaag atctcgatgt cgtgatcgat ctggtcaacg atcggcccga gacgagtttg
     3721 ttgctgaacc gcctctcctc gggatacttc atcgatcttc cagccgcgat agatgcgatc
     3781 gaacaccgga gtctgtttca ggcgatcgat gaacggtcga tggtcaaaat tgatttccat
     3841 gtcggcgaga agatttctgg cgaactggac cgcgcaactc gccgtgaagt tgttccgggc
     3901 attatggctc ccctcgtcag caaggaagac gccatcctct ccaagctact ctggatccag
     3961 caaggcagcg gcaagtcccg gcacgatgtg atcgaaatgc tgaaacgcga cgaagacctc
     4021 gaccgcggtt gcctccggga ccgggccgcc acgctcgggc tcagtgagct gctagcagaa
     4081 ttcgaaaatg agatctgatt gacggaggcc ggtgaaacca ggccctcgat cgaagaacct
     4141 gacaccaagt cgttcaccgg agagccgtcg atgcctgttt ccgtgttatg gggccagacc
     4201 ctgtttgccg cgagtcttct ctgttggaca ttcaacctcg cccacggtga cgagccggtg
     4261 aaaccgacgg cgccggcctc cgagaccaac ccggccgttc ccgcccccgg ccattcagta
     4321 cacggcgagg cgttcaatga cggaccgcgg catgccgcct accgcatgcc cggaatgggc
     4381 aagctgcact tcccggttgc gaccaagcag cccgaagccc aggctttcat cgatcaaggc
     4441 gtcgcgcagc tccattcttt ctattacttc gagtccgagc gatcattccg tcaggccgcc
     4501 aaaatcgatc c
//
LOCUS       DATAQL010001272         4944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3641773, whole genome shotgun sequence.
ACCESSION   DATAQL010001272 DATAQL010000000
VERSION     DATAQL010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641773"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..354)
                     /locus_tag="VHS97_21910"
     CDS             complement(7..354)
                     /locus_tag="VHS97_21910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450926.1"
                     /translation="MHPNDETLKGPQIVFALYKPHDGKDADLRQILAEHVPTLRRLEM
                     ATDRPTVLVKSKNGTYVEIFEWSTPESARLAHHHPEVAKVWEAMGKVADLAQLDSLEE
                     VKGMFPHFEPVDL"
     gene            complement(338..676)
                     /locus_tag="VHS97_21915"
     CDS             complement(338..676)
                     /locus_tag="VHS97_21915"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3450927.1"
                     /translation="MTRSRGERELEDLEVVFKALAHASRRHVLVVLNARGGSMTAGEI
                     ARRFSCSWPTTSRHLRVLEQAGLVRVDKQGREWVYVLKTDRIRQVVGGWIRWFEPSIE
                     EKGKEDASER"
     gene            998..1582
                     /locus_tag="VHS97_21920"
     CDS             998..1582
                     /locus_tag="VHS97_21920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3450928.1"
                     /translation="MMSPTSVSLLDRLKAARPDASDWNRLQGIYLPLIGRWLGRVPGV
                     GDEADDLAQEVFVVVIREIPRFRRQREGSFRAWLRQVTVNKVRTHRRRRHRRPLIGLD
                     PADGFLDRLADPNGDLAREWDMDHDRHVFEKLLAVVQPDFSATTWEAFRRFAVDGLPA
                     AVVAEEMGMSVNSVIQAKSRVLKRLREEAGDLLA"
     gene            1681..4140
                     /locus_tag="VHS97_21925"
     CDS             1681..4140
                     /locus_tag="VHS97_21925"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3450929.1"
                     /translation="MGAAAPVHPNDQTIQSFGLGKLDDALAASIQRHLESCTDCRRRV
                     AEVTSDSFLGRLSAAEGRADSSGPLVTTDTCLSTLAANASSTASSLADTIPGGLVNHP
                     DYEVIRELGQGGMGTVYLAKNRLMGRLEVLKVVSSHLIKRRGVMDRFLSEIRNAARLH
                     HSNIVTAYSASRLGESIIFAMEYVDGLDLAKLVKAKGPFAVAHACNYVYQAALGLQHA
                     HEHGMVHRDIKPSNLILAPQGGRAVIKVLDFGLAKIQSEGAVDGGLTHDGQMLGTPDF
                     VAPEQIGDARRADIRADIYSLGCTIYYLLTGGPPFKATSLYEILQAHHSMDALPLNLV
                     RPDVPVKLAALVARMMAKDPELRFQTPNEVAQALKPFFKPASFGSVKPELSFPGSPDV
                     VGDLRAPALPPATNKPDHLPASRPAVAVPGAGSVWERLIDLGEPDPLSDAPPVIAATA
                     RPTRPWPMVIGVSIVAILVVTALAWLVGHMTTTDDQNVSVALRAENRVLQSPNTKSPG
                     VTLKDETSGFTQTDASFGGPTPVSPRDPFPSSSANADNRFERAPSTVEQKSSSNTPAQ
                     STSQVKAETPEQAAQKPRTESPVSVAEVLSPDERLQQFGLKRTGRIYFLTRETDVERR
                     FNEFRSTLEEFRTNAQKKAANETGLATMKELEQANSKRNLWIDQLNQELSKQPPRPDN
                     QVKATFDAMRAERDQHELDRSAASFLWKELQGQLLGPREMYEINVAIERGRVSCRSAL
                     DELGELIKTTDAKIRGLKTNDQVKKALADLKDLRLDHSSGYQNTVKQIRQWDVFVKST
                     TERTEPKAEPVAKKKSSMPKR"
     gene            4264..>4944
                     /locus_tag="VHS97_21930"
     CDS             4264..>4944
                     /locus_tag="VHS97_21930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3450930.1"
                     /translation="MGVTAAVHPTDQVLQSYGLGKLDDASSVSVSKHLEGCDSCQRRV
                     AELSCDEFLGRLRYVQAKPDKSATSWSPSAASSHEGIWRPAVPPPPVDTLPPELVTHP
                     DYEIVRELGRGGMGVVYLAQNKLMGRPEVLKVVGGHLVERPGVRDRFLREVHSAAKLQ
                     HKNIVTAYSAMRLGESIVLAMEYIEGDDLAKVVKSSGPLLVMNACYVIYQAALGLQHA
                     HERGTVHRD"
BASE COUNT         1030 a         1455 c         1493 g          966 t
ORIGIN      
        1 gttaaatcac aagtcgacgg gctcaaaatg cgggaacatg cccttgacct cctcgaggct
       61 atcgagctgg gccaggtcgg cgactttgcc catggcctcc cagactttgg ccacctccgg
      121 gtgatggtgc gccagtctgg ctgactcagg cgtgctccac tcgaaaatct cgacgtacgt
      181 cccgttcttg gatttgacca gtacggtcgg ccggtccgtg gccatttcca accggcggag
      241 cgtcggtacg tgctcggcga gaatctggcg tagatcggcg tccttgccat cgtgcggctt
      301 gtagagtgca aagacgattt gcggtccctt cagcgtttca tcgttcggat gcatcttctt
      361 tccctttctc ctcgatcgag ggctcaaacc aacgtatcca cccaccgacc acctggcgga
      421 tgcgatctgt tttgaggacg tagacccact cccgaccctg tttgtcgaca cgcaccaagc
      481 ctgcctgttc gaggactcgc agatgacggc tggtcgtggg ccatgagcaa gagaaacggc
      541 gcgcaatctc cccggccgtc atgctcccgc cgcgcgcatt cagtacgacc aacacgtgac
      601 ggcgcgaggc gtgcgcgaga gccttgaaca caacttccag atcctcgagc tctcgttccc
      661 cacgacttcg tgtcattgag cggtttctct cagcagcatc cccaaatagt tagcagatgc
      721 gctaatcgtc gtcaagtacc tcgcgctgaa atgtcttgtt acgaagtgaa gaatccgctt
      781 aagtaaactc gtgtagagac ctttcgtatt catagtagtg ggcaactggg taccgaagct
      841 accttcttgt gttgatgtat tgtttgtgac agactcgaaa acttagactt cgttgttcaa
      901 aaaatgcgat gacggcattg aggcggttag gcaagcagat gtgcgcggag ggcacctggc
      961 ggaagttgcg ccggcgaaaa aggtgaacaa cacgaagatg atgagcccga cctccgtcag
     1021 tcttctcgat cggctcaagg cggcccggcc ggacgcctcc gactggaacc gtctgcaggg
     1081 catctatttg ccgctgatcg gtcgctggct aggccgagtg ccgggagtgg gcgacgaggc
     1141 cgatgatctg gctcaggaag tgtttgtggt ggtgattcgc gagattcccc gattccgtcg
     1201 gcagcgcgag ggatcatttc gagcatggtt gcggcaggtg acagtcaaca aggtccgcac
     1261 ccatcgccga cgccgccatc gccgtccatt gatcggtctg gatccggccg acggattcct
     1321 cgacagactg gccgacccca acggtgacct cgcccgtgag tgggacatgg accacgaccg
     1381 ccatgtcttt gagaaattgc tcgcggtcgt gcagccggat ttcagtgcga cgacttggga
     1441 agcgttccgc cgattcgccg tcgacggcct accggcggcc gtcgttgccg aggaaatggg
     1501 tatgagcgtg aattcggtga ttcaggccaa gtcgcgagtt ctcaagaggt tacgtgagga
     1561 agccggcgac ctgctggcgt aattttattt tttcgcggca ggtgtggcag tgtcgccgcc
     1621 tttctctatt agtcattgac cgacgatcgg tgaacatgac cctgactagg agattgcacc
     1681 atgggcgctg cagccccggt ccatcccaac gaccagacca tccagtcgtt cggtctgggt
     1741 aagctcgacg atgctctggc tgcgtcgatc caaagacatc tggaatcgtg taccgactgc
     1801 cggcgccgcg tcgcggaggt aacttccgac agctttctcg gtcggctcag cgccgcggaa
     1861 ggccgcgccg attcgtcggg tccgctggtc accacggata cgtgcttgtc cacgcttgcc
     1921 gcaaacgcga gctcaacggc atcttcgctg gccgacacca tacctggcgg actggtgaac
     1981 caccccgact acgaggtcat ccgcgaactc ggccaggggg gcatgggaac tgtctatctc
     2041 gccaagaacc gactcatggg acggcttgag gtcttgaagg tcgtcagcag ccatttgatc
     2101 aagcggcgcg gggtgatgga tcgctttttg agcgagatcc gcaacgcagc ccggctgcac
     2161 cactccaaca ttgtcactgc ctattcggcc agccgcctgg gtgagagcat catctttgca
     2221 atggagtatg tcgatggtct cgacctggcc aagctggtca aagccaaagg accattcgcg
     2281 gtagcgcatg cctgcaatta cgtctatcaa gcggctttag ggctgcagca cgcgcacgag
     2341 cacggcatgg ttcatcgcga catcaagccg agcaacctga tcctggcgcc gcagggggga
     2401 cgtgccgtga tcaaggtact ggacttcggc ctggccaaaa tccaaagcga gggtgcggtc
     2461 gatggcggct tgacccacga cggtcagatg ctgggcacac ccgacttcgt tgccccagag
     2521 caaatcggcg acgcgcggcg ggccgacatc cgagcggaca tctacagcct gggatgcact
     2581 atttactatc tgctgaccgg cgggcccccg ttcaaggcga cgagtcttta tgagatcctg
     2641 caggcccatc attccatgga cgcgctgccg ttaaatctgg ttcgaccgga cgtcccggtg
     2701 aagctggcgg cattggttgc caggatgatg gccaaggatc cggagctgcg attccagacg
     2761 ccaaacgagg tggcgcaggc actcaagccg ttcttcaagc cggcgagttt cggcagtgtg
     2821 aagccggagt tgtcgtttcc cggatcgccg gacgtggttg gtgacttacg cgcaccggcg
     2881 ctacctccgg cgacgaacaa gccggatcac ctccccgcct cgaggccggc ggtagcggtg
     2941 ccgggagccg ggtcggtgtg ggagcgattg atcgaccttg gcgaaccgga tccgctgagc
     3001 gacgcgccgc cggtcatcgc ggcgacggcg cggccgacgc gtccctggcc gatggtcatt
     3061 ggagtctcga tagttgcgat actggtggtc accgccctcg cctggctggt cggacacatg
     3121 acaacaactg acgaccagaa cgtgtcggtg gctctgcgcg ccgaaaacag ggtgctgcaa
     3181 tccccgaaca ccaaatcccc gggtgtgacg ctaaaggacg agacttcagg cttcacccag
     3241 acggacgctt ccttcggcgg tcccacccct gtgagcccgc gagatccttt cccgtcctcg
     3301 tcggctaatg cggacaaccg ctttgaacga gcgccgtcga ctgtcgaaca gaaatcatcg
     3361 tccaacacgc cggcgcaatc gacatctcaa gtcaaggcgg aaacgccgga acaagcagct
     3421 cagaaaccgc gtaccgaatc gccagtttcc gttgctgagg ttctttcacc ggacgaacgg
     3481 ctgcagcaat tcggactgaa gcggacgggc aggatctatt ttctgacgcg ggagacagac
     3541 gtcgaacgaa ggttcaatga atttcgctcg acgctcgagg aatttcggac caatgcgcag
     3601 aagaaagcgg cgaacgaaac cggcttggcg acaatgaaag agctggagca agccaacagt
     3661 aagcgaaatc tttggatcga ccagctcaat caggagctgt cgaagcaacc ccctcgcccc
     3721 gataatcaag tgaaggcgac ttttgatgcg atgcgagccg aacgcgacca gcacgaattg
     3781 gacagaagcg cggcctcatt tctttggaag gagcttcagg gccaactgct cggtccgcgg
     3841 gagatgtacg agatcaacgt cgcgatcgaa cgcggccgag tttcgtgtcg atcggcactg
     3901 gatgagctgg gcgagctgat caagactacg gacgcgaaaa tccggggtct caaaacaaat
     3961 gaccaggtca agaaagcctt ggcagacctc aaggatctca gactggatca ctcatctgga
     4021 tatcaaaata ctgtcaaaca gataaggcaa tgggacgtat ttgtgaagtc gacgactgag
     4081 aggacggaac cgaaggccga acccgttgcg aaaaagaaat ccagcatgcc gaaacgttag
     4141 ggtctcaaat gggaagattg ccgagaaatc cccatcttca cgacaggttg cgtggcatga
     4201 cggcctttct ctattagtga ttgatcgacg attggccttt gattccgacg aggagatcga
     4261 gcaatgggcg tcaccgctgc cgtccacccg actgaccagg tcttgcagtc ctacggcttg
     4321 ggcaagctcg acgatgcctc atccgtgtcg gtgagcaagc acctggaagg gtgcgattcc
     4381 tgccagcggc gggtcgccga gctctcgtgc gacgaattct tgggacggct tcggtacgta
     4441 caggccaagc cggacaagtc ggcgaccagt tggtcgccgt ccgctgcatc gtcccatgaa
     4501 ggcatctggc gacctgccgt cccgcccccg ccggtcgaca ctctgcctcc cgagctggtc
     4561 acccaccccg actacgaaat cgtccgcgag ctgggacgcg gcggcatggg agtagtctac
     4621 ttggcccaga acaagctgat gggacggcca gaggtgctca aggtcgtagg cgggcacttg
     4681 gttgagcggc ccggcgtgcg cgaccggttc cttcgagagg tccattcggc cgccaagctt
     4741 cagcacaaaa atatcgtcac cgcgtactcg gccatgcggc tcggcgagag catcgttctt
     4801 gcaatggagt acatcgaggg ggacgacctg gccaaagtgg tgaagtccag cggcccgctc
     4861 ctagtgatga acgcctgcta cgtcatatac caggcggccc tcggtttgca gcacgcccac
     4921 gagcgcggca cggtccaccg tgac
//
LOCUS       DATAQL010001273         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2422112, whole genome shotgun sequence.
ACCESSION   DATAQL010001273 DATAQL010000000
VERSION     DATAQL010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422112"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VHS97_21935"
     CDS             <1..714
                     /locus_tag="VHS97_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3450931.1"
                     /translation="TSGIGRAVALALAHAGADVIVHGRTSRDQAEAVAALVRARGGRS
                     AVLLADLADRAAGDRLVADAWSVWDGLDAWLHIAGADTLTGAAAKLPFDAKLDLLLSV
                     DVAATIRLCRAVGRLMADQGSGAIVTMGWDQAETGMEGDSGELFAASKGAVMSFTRSL
                     ALSLAPAVRVNALAPGWIKTAWGEGASRIWQDRVLKETPLARWGTAEDVAQVARFLVS
                     PAAGFLTGQVIRINGGSVR"
     gene            727..>1685
                     /locus_tag="VHS97_21940"
     CDS             727..>1685
                     /locus_tag="VHS97_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HEX3450932.1"
                     /translation="MNQETRSNSPRYLFVTGRLAEFALRKMLDDLAPRAGFTAQVAVL
                     PITVAALMTPRWIARHLVVPEGIDRVILPGYCVGDLAPIVEQARGAPVEPGPEDLRDL
                     PRYFDHACESNIPYGQYDIEILAEINHAPRLERQELLRQADRFRSQGADGIDLGCVPG
                     TTWTEAGDAVSALRDQGIRVSIDSFDPTEIAHAVSAGAELVLSVNATNRAHAPDWGVE
                     VVAIPDQPGSLTGLDETIEFLLRRKVPFRIDPILEPIGFGFAASLHRYFDIRDRYPDA
                     AMIMGVGNLTELTDVDSAGVNTVLIGICQELTIQSVLTTAVIN"
BASE COUNT          283 a          547 c          545 g          310 t
ORIGIN      
        1 acgtccggga taggccgcgc cgtcgccttg gccttggccc acgccggggc cgacgtgatc
       61 gtgcatggac gcacttcgcg agatcaggcc gaggcagtcg cggcccttgt gcgcgcacgc
      121 ggcggccgct cggcggtgct cctggccgat ctggccgacc gagcggccgg cgatcgcctt
      181 gtcgccgacg cctggtccgt ctgggatgga ctcgatgcgt ggctccatat cgccggtgcc
      241 gacacgttga ctggcgcagc cgccaagctt cccttcgacg ccaagctcga tttacttttg
      301 tcggtcgatg tcgccgcaac gatccgcctt tgccgcgccg tcggtcgact catggcagac
      361 cagggttcgg gtgcgatcgt gacgatgggc tgggatcagg cggagaccgg catggaggga
      421 gattcgggcg agctgttcgc cgcgtcgaag ggagccgtca tgtcgttcac ccgcagtctg
      481 gcactcagtc tggcgccggc ggtccgggtc aacgcgctgg cccccggctg gatcaagacg
      541 gcctggggcg aaggtgcctc tcgaatctgg caagaccgag tgctcaagga aacccctttg
      601 gcccgctggg gcacagccga agatgtcgcg caggtcgccc gcttcctggt cagcccggcc
      661 gccggattcc tcaccggcca ggtgatccgc atcaatggcg gatcagtgcg ctgaaagggg
      721 ccacgagtga atcaagagac ccgctcgaat tccccccggt accttttcgt gactggccgg
      781 ctggccgagt ttgctttgcg gaagatgctc gatgatctgg ctccgcgagc cgggttcacc
      841 gcccaagtcg ccgtcttgcc gatcacggtg gccgcgctca tgacgcccag gtggatagcg
      901 cgccacctcg tcgtccccga aggaatcgac cgcgtgatcc tgccgggata ctgcgtgggc
      961 gatctagctc caatcgtcga gcaagcacgg ggcgcacctg tcgagccagg gccagaggac
     1021 ctgcgcgacc tgccgcgcta ctttgaccac gcttgcgaat cgaacatccc ttacggtcaa
     1081 tacgacatcg agatccttgc cgaaatcaac catgccccgc gtctcgaacg tcaggaactg
     1141 ctcaggcaag cagatcggtt ccgctcacaa ggggccgacg gcatcgacct gggttgtgtc
     1201 ccgggcacga catggacaga ggccggcgat gccgtctcgg cattgcgaga ccaaggcatc
     1261 cgtgtctcga tcgacagctt cgatccgacc gagatcgcgc atgcggtctc ggcgggagcc
     1321 gaactcgtcc tgagcgtgaa tgcaacgaac cgggcgcacg cccccgattg gggagtggaa
     1381 gtcgtggcga tccccgacca accgggatcg ctgacgggtc tggatgagac gatcgagttt
     1441 ctgctccggc gcaaggtgcc gtttcgcatc gacccgattc tcgagccgat cggctttggc
     1501 tttgctgcct ctcttcaccg ctatttcgat atcagggacc gctaccccga tgcggctatg
     1561 atcatgggtg tgggcaacct caccgagctc accgacgtcg attccgccgg cgtcaacacg
     1621 gttctgatcg ggatttgcca ggagctgacg atccagagtg tactgacgac cgccgtgatc
     1681 aactg
//
LOCUS       DATAQL010001274         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1350830, whole genome shotgun sequence.
ACCESSION   DATAQL010001274 DATAQL010000000
VERSION     DATAQL010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350830"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1642)
                     /locus_tag="VHS97_21945"
     CDS             complement(251..1642)
                     /locus_tag="VHS97_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450933.1"
                     /translation="MPLGRGAPFFAAPRPGRPTQAPTPEPEGPEAADRGCLDLEPGRR
                     LRTPVAGIFLFLPLLARLRFDRLVAQAGYPGSQMIPADAALLSLPALKLLDKERRSHI
                     SDFNCDEGLGLFAGLNVLPKTAYATEYSYRTQRDQQQRLLSGWITALAPLLFPEGRSF
                     ALDFHTIPFRGDPAALENHHLPRRGKAGPSVLSFFAQEQGSRVVCYANANLARADQPG
                     ELMRFVEFWHQVAGHDPLWLIFDSKVVPYSGLSRVNRRGIHFVTIRRRGAAVIRRLRG
                     LPPRAWQRAVIDTPHRRHQRIRFVDESIRLPDYEGAIRQLAVDGLGREQPTLFLSNDL
                     EETARALVIRYAGRNGVEDGLGTSVNFFHLDCLASQVRLNVDLDVALTVLAHGCYRWL
                     AHQLKGFDRSAPKRVFRKFVATAGLVTIGEKGIAVRFDRRSHNPILREAGLDRECPAV
                     PWLNNLPITFAYR"
     gene            1696..1836
                     /locus_tag="VHS97_21950"
     CDS             1696..1836
                     /locus_tag="VHS97_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450934.1"
                     /translation="MGGDLLEGTPLDEVGPQRLIASVEGVVWLQEEAEAGGVVHDRGS
                     EM"
BASE COUNT          383 a          647 c          778 g          358 t
ORIGIN      
        1 acctgtaata gatattttgt cccatccctc cttgaggcgt ttggtcacgc cgctctcctt
       61 accaggtgcc cggctcaata gcgaaaccgc gacccgccgg atcatcgcca ggttggagcc
      121 cgcatggtcc tggcggattc ggctccgatc cttgcggaag accacgttca ggacccaatg
      181 gagtccattc tccgcggttt tccatgggtg gaaatcccct cacagtagga caactgtcag
      241 gtggcgatcg tcacctataa gcgaaggtga tgggtaggtt gttcaaccat gggacggcgg
      301 ggcactcgcg atccaacccc gcctcccgaa ggatcgggtt gtggctccgg cggtcgaacc
      361 ggaccgcgat ccctttctcg ccgatggtta ccaggccggc cgtcgcgacg aatttccgga
      421 acacccgctt cggcgccgag cggtcgaacc ccttcagttg atgcgccagc cagcggtagc
      481 aaccgtgggc cagcaccgtc aatgccacgt ccaggtccac gttcaaccgc acctggctgg
      541 ccaggcagtc gaggtggaag aagttgacgc tggtccccaa gccatcttcg acgccgttcc
      601 gcccggcata acggatgacc agcgcgcggg ccgtctcctc gaggtcgttg gacaggaaca
      661 acgtgggctg ctccctcccc aggccatcga cggccagttg ccggatcgcc ccttcgtaat
      721 cgggcaggcg gatgctctcg tccacgaatc ggatgcgttg atgacggcga tggggcgtgt
      781 cgatgacggc gcgctgccag gcccgcggcg gcaggccgcg caggcgacgg atcacagcgg
      841 cgccacgccg gcggatcgtg acgaagtgga tgccgcgccg gttgacgcgc gacagtccgg
      901 aataggggac caccttggaa tcgaagatca gccagagggg atcgtgcccc gcgacctgat
      961 gccagaactc gacgaaccgc atcaactccc ccggctgatc ggcgcgggcc aggttggcgt
     1021 tggcgtagca cacgacccgg ctgccttgct cttgggcgaa gaagctcagg acgctgggac
     1081 cggccttccc gcggcggggc aggtggtggt tctccagggc cgcgggatcg ccccgaaagg
     1141 ggatggtgtg gaagtccaag gcgaaggacc gcccctcggg gaacaggagt ggcgccaggg
     1201 ccgtgatcca gccggagagg aggcgttgtt gttgatcccg ctgggtgcga taggagtact
     1261 cggtggcata agcggtcttg ggcaggacgt tcaggccggc gaagaggccc aggccctcat
     1321 cgcagttgaa gtcgctgata tggctccgcc gctccttgtc caggagcttc agggccggga
     1381 ggctcagcag ggccgcgtcg gccgggatca tctgcgagcc ggggtagccc gcctgggcga
     1441 cgaggcgatc gaagcggagt cgggccagca gcggcaggaa gaggaagatg cccgcgacgg
     1501 gggtgcgcag tcgccggccc ggctccaggt ccaggcatcc tcgatcggcc gcctcgggcc
     1561 cctcgggctc gggagtgggg gcttgggtcg ggcgtccggg gcgcggggcg gcgaagaaag
     1621 gggcgccgcg cccgagcggc actgggtccg gaactggtgg atgacctggc gcggggtgtc
     1681 gtaggtgtat ccgaaatggg tggcgatctc ctggagggga cgccgttgga cgaagtaggc
     1741 ccgcagcgcc tcatagcgtc ggtggagggt gtcgtctggc tccaggaaga agcggaggca
     1801 ggaggagtcg tccacgaccg gggctccgag atgtgagggg gtttcgcctt gggtgtcggt
     1861 cgtcgatcat attgatgatg gctgatagga ccaagggagt accctcgcga gccgtcggag
     1921 ggaggggaaa cgggctgtgg ggtcgagcga tctgcgagat acaggaaggg atgttcaacc
     1981 gaaaggatca gtgtcgaggg gcgcttctgc cgaggaatcc cctcacaagg ggtcgcggca
     2041 gaacctgaaa tcccgcggga aaacaaaggc gatccccctc ggattgtgag ggggtttcca
     2101 tccatggaaa tccgcgttct cgatatccca gtgcccccgg acatatccgg cgatctcggc
     2161 cgccgt
//
LOCUS       DATAQL010001275         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2266459, whole genome shotgun sequence.
ACCESSION   DATAQL010001275 DATAQL010000000
VERSION     DATAQL010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266459"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /gene="larA"
                     /locus_tag="VHS97_21955"
     CDS             complement(<1..894)
                     /gene="larA"
                     /locus_tag="VHS97_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX3450935.1"
                     /translation="MKKMKLKLDYGRTGLDVELPTDRLVGPLAIRDVPPLPDPEAAVA
                     IALQDPIGAPSLREIARGRNDACILICDITRPVPNQTILRPMLEVLAQAGIPRERTLI
                     LVATGLHRPSTAAEKLEMLGEEIVAGYRVEDHYGTRLDEHTLVGTTARGIPGWIDSRY
                     VRADLKIATGLIEPHLMAGYSGGRKLICPGVAALETVKRWHGPELLEHPKADCGILEG
                     NPVHEENTAIARMAGCDFIVNVTLDSQRRVTSVVAGDMEQACHEGVRFMAGVCRALVP
                     KPVEIVVTSSAGYPLDTTFYQA"
     gene            complement(961..1806)
                     /locus_tag="VHS97_21960"
     CDS             complement(961..1806)
                     /locus_tag="VHS97_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3450936.1"
                     /translation="MIARTQSHSRSAIVAGLAMLTGSIATATDPPQGTSQAQQFPAQE
                     GWKALFNGKDLTGWIARNPRAKKTWVVCDQARLDPADPGRLLPIGKGGSADSVLLCGE
                     DGRGSDIMTEQKFGDYELHIEFTVPKGSNSGVYNRGLFEIQVFDSFGKPKLGWHDCGA
                     LYERAFPRDNFAKPPGDWQSYDITLKGKKLSMAWNGRSVYRDHDVRYGETDRDAFERL
                     NQENASRPEGLRVKLREENGRYVGYFGEGGTRSGLDGPDRPGPILLQGDHGPVAYRNI
                     MIRLR"
     gene            1948..2991
                     /locus_tag="VHS97_21965"
     CDS             1948..2991
                     /locus_tag="VHS97_21965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3450937.1"
                     /translation="MSQPTKPNRNVSPSRREFMQAGAAGLATAALATAGAESAHAQNA
                     AGIPLRPLGSTGQSVTLIALGGFHSSVHSNENDSIRLIQRAVDEGITFLDNAWDYHDG
                     RAEERMGKAIAQGNRRDKVFLMTKCCGRTAKDAQSNLEDSLRRLQTDHLDLWQFHEIV
                     YDNDPDWIFDKAGAIEYAVKAKEQGKIRFLGFTGHKDPAIHLKMLDKPYPWATVQMPL
                     NVMDGHFRSFQHRVLPVLSQRGIAPIAMKSLGGTGKIVSESVVSIDEALRYVLSLPIA
                     SLVSGIDSESVLEQNLKIVRDFKPMTAEEKAAVETKVRPYAGDGRFELFKSSKVFDGA
                     HHRKQHGFSTEIS"
BASE COUNT          673 a         1124 c         1095 g          676 t
ORIGIN      
        1 cgcctggtaa aacgtagtat cgagcggata acccgcggag ctcgtgacca cgatttcaac
       61 cggcttcggc acaagtgcgc ggcagacgcc cgccatgaag cgaacgcctt catggcaggc
      121 ctgttccatg tcaccggcca ccaccgacgt gacccgccgc tggctgtcga gcgtcacgtt
      181 gacgatgaag tcgcagccgg ccatgcgcgc gatcgccgtg ttctcctcat gcaccgggtt
      241 gccttcgagg atgccgcaat cggccttggg gtgctccagc agctcgggcc cgtgccaccg
      301 tttgacggtt tcaagtgcgg ccacgccggg acaaatcaac ttgcgacccc ccgaataacc
      361 ggccatcaga tgcggctcga tcaggccggt cgcgatcttc aagtccgccc ggacgtaacg
      421 gctgtcgatc catccgggaa tcccccgcgc ggtcgtgccg accagcgtat gctcgtcaag
      481 gcgcgtccca tagtggtcct cgactcgata gccggcgacg atttcctcgc cgagcatctc
      541 gagcttctcg gccgccgtgc tgggccggtg caatccggtc gcgaccagga tgagcgtccg
      601 ttcgcgcgga attcccgcct gagcgagaac ctcgagcatt ggccttaaga tcgtctggtt
      661 gggaaccggg cgcgtgatat cgcagatcaa gatgcaggcg tcgtttcgcc cccgggcgat
      721 ctcgcgtagg gaaggcgcgc cgattggatc ttgcagggca atcgcgacag ccgcttcggg
      781 atcgggcaac gggggaacgt cgcgaatcgc cagcggaccg acaagccggt ccgtgggaag
      841 ctccacgtcc aaacccgtcc gcccgtagtc cagcttgagc ttcatctttt tcatggtgtt
      901 gatcgtccca ggtcaggccg aagttcagac gtctctgtca ggcggatatc caagcttgtt
      961 ctagcgcaat cgaatcatga tgttccgata ggcgaccggt ccgtgatccc cttgcagcaa
     1021 tatgggcccg ggacggtcgg ggccgtcgag acccgaccgc gttcccccct cgccgaagta
     1081 gccgacgtat cgcccattct cctcacgaag cttgacgcgc aacccttcag gcctgcttgc
     1141 gttttcctgg ttgaggcgct cgaacgcgtc gcgatcggtc tcgccgtagc ggacgtcgtg
     1201 gtcgcggtac acggatcggc cgttccaggc catggagagt ttcttgccct tgagcgttat
     1261 gtcgtacgat tgccaatcgc cgggcggctt cgcgaagttg tcgcggggga aagcccgctc
     1321 gtaaagcgcc ccgcaatcat gccagcccag cttgggcttg ccgaagctgt cgaagacctg
     1381 aatctcgaac aggccgcggt tgtaaacccc agagttgctc cccttcggaa cggtaaactc
     1441 gatatggagc tcgtagtcgc caaacttctg ctccgtcatg atgtcggacc cgcgaccatc
     1501 ctctccgcaa agcaaaacgg aatcagccga ccctcctttg ccgatgggaa ggagacggcc
     1561 cggatccgcg ggatcgagcc gcgcctggtc gcacacgacc caggtcttct tggcacgagg
     1621 attgcgagca atccaacccg ttaagtcttt gccattgaag agcgccttcc agccttcctg
     1681 ggctggaaac tgttgcgctt ggcttgtgcc ttgcggcggg tccgtcgccg tggcgatcga
     1741 gccggtcagc atggccaaac cggccacgat cgcggagcgc gagtgcgatt gggtccgtgc
     1801 aatcatcttg tttcctcttc gcaatggact cggtgtggac gagaccgtcg gcgctgctcg
     1861 tgatcgacaa tcgcctgtca acgaggttac catctcgccg gagggttgtc ccgagattac
     1921 ccaacacctg aaaggcttga tgccagcatg tcgcaaccaa ccaagcccaa tcgaaacgtc
     1981 agtccgagcc gccgcgaatt catgcaggcg ggggcagccg gtctggcgac ggcagcgctc
     2041 gccacggcgg gagccgaatc ggctcatgcc cagaacgccg caggaatccc gctgcgcccc
     2101 ctcgggtcca ccgggcagtc cgttaccttg atcgcccttg ggggcttcca cagtagcgtc
     2161 cattccaacg aaaacgacag cattcgtctc atccaacgcg cagtcgacga ggggatcacg
     2221 ttcctcgaca acgcctggga ttaccacgac ggccgtgccg aggaacggat ggggaaggcg
     2281 atcgcgcagg ggaaccgccg tgacaaggtt ttcctcatga ccaagtgctg cggccgaacc
     2341 gccaaggacg cccagtcgaa cctggaagac agcctccgcc ggctgcaaac cgatcatctc
     2401 gatctttggc agttccacga gatcgtctat gacaacgacc cggactggat ctttgataaa
     2461 gcgggcgcga tcgaatatgc agtcaaggcc aaggaacaag gcaagatccg cttcctgggc
     2521 ttcaccggtc acaaggatcc ggcgatccac ttgaagatgc tcgacaaacc ctacccatgg
     2581 gccaccgtgc agatgcccct gaacgtgatg gacggccact tccgcagctt ccaacaccga
     2641 gttttgcccg ttcttagcca gcgagggatc gcgccgatcg cgatgaagag cttgggcggc
     2701 accggcaaga tcgtgagcga gtccgtggtg tccatcgacg aggcgctgcg gtatgtgctg
     2761 tcgctgccga tcgcatcgct cgtttcgggc atcgactcgg aaagcgtcct ggagcaaaac
     2821 ctcaagatcg tccgcgattt caagccgatg actgccgaag aaaaagccgc cgtcgagacc
     2881 aaggtgcgac cttacgccgg cgacgggcgg ttcgagctct tcaagagctc gaaagtcttc
     2941 gacggtgcgc atcatcgcaa gcagcacggg ttttcgacag agattagctg accggatgac
     3001 acgggccaac ggctatgacc tcgctgagtt atgatcaaga tttcgatgga ccaattccgt
     3061 attcgcagga tggcactatt tggcactatt tggcagtgat ggggcccgac ctcgggatcg
     3121 acgtcggtgg gctgtgtccc ccacccgcag cgagaccgaa ctgagcgagg cccagggtcg
     3181 tcgcagtttt gtggcccttg gacgagctgt actttccgtt acttcagcta ttgagcttgc
     3241 cgctgatctg gactttctgc gacatgattt gcgtcatgca gccgggccgg gaagccggtc
     3301 ggccgccggt gtcaccgggt cgccgttgga acaggacttg tatcaggccg cccgatttgt
     3361 ggataggcac tcagcatggc ctgggcggtt gcgcaatggt cggcggggcg cgcgagaagg
     3421 cgtcgccctc ctgttccggc gggggcgacg cccgatcgtc ccgtgagggc ggtcacagga
     3481 tgacgaacgg gttgatgtcc atccggaacc ggccccggcc aggcgcatcg ggggcggtca
     3541 gttccagcac gccgcccgtg ccctccag
//
LOCUS       DATAQL010001276         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_435080, whole genome shotgun sequence.
ACCESSION   DATAQL010001276 DATAQL010000000
VERSION     DATAQL010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435080"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2407
                     /locus_tag="VHS97_21970"
     CDS             <1..2407
                     /locus_tag="VHS97_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX3450938.1"
                     /translation="NRNSEHAAGRPTVEPAKATLRISPESYTIAADDPGLQLLAARNE
                     SGKVRDLTSQVAWTIEPPGMAELEPGGYLRPVGPGVVTVKAALEGQSVTARITLEPRS
                     TRSWDFAEDIVPVFTRLGCNTGACHGKADGQNGFHLSLFGYDRAGDFQALARDGGQRR
                     LSGLVPEASLFLTKATGTVPHAGGRRLTVGSPEYQTLLAWVKDGAPERRGTTHGPVVK
                     VAVEPGSEPFAEPGLRQLRVTAHYEDGHQRDVTRLATYRVNDDAAGSVTPQGQAALLR
                     RSETDLIVRYQSYVLNTRLGTVINPELAFDFGKLRRTNFVDDELFKRLEALKVPPSPR
                     AGDAAFLRRVSLDLTGEQPAPEDVRRFLADHDPDKRTKLVDRLLAQPEFVAFWRIKLG
                     DLLQISSARHGNGAYRYQAWIDRCLNKNEPWDKVVSTLLTAVGDPNDIETGGPVNYVM
                     DTFEPNVQAEQTAQRFLGLRIRCAQCHDHPFDVWTQDAYYGLASFFAKVQRSGRGAMM
                     GRPTVTINPDGKVVHLRTKQPVEPRLLDGRAVAVPAKDDPRKVLASWMTAPENPYFAR
                     ATANWVWSQLFGKGLVDPPDDMSRANPAVHPELLDALARHFVATKFDLRALVRTIAVS
                     ESYGLSSATVPGNERDTRLFSHQLPRPLTAHQMADALAQATDVANRFQNVARRAIDLP
                     DPMSASTILDTFGRCARTTACASTPAPALSLRQALLLIGGDVVEGKVSNLNGYLASAL
                     KLELEPEELVENLYFRTVCRPPTAEELSHWSAELKQAASKREAAEDLFWALLNSREFA
                     FNH"
     gene            2504..>3215
                     /locus_tag="VHS97_21975"
     CDS             2504..>3215
                     /locus_tag="VHS97_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3450939.1"
                     /translation="MSSIPSRQPCRGPNRREVLRIGSLGFLGLGLDEWLRFTARANST
                     KASDSVKVKNCILIWLAGGPSHIDTFDPKPEAGVDVRGEFKPIATSVPGLQISEVFPN
                     LAKVMDRVALIRSMTSPEADHDRASHHMLTGYRPTPALVYPGYGSVVSKTREATRGML
                     PPFVAIPDAPIYASSGFLTPAYDPFTVSGDPNQESFRVQNLTPPDKLTLERLLRRRDM
                     VRKLDDFAHDVPGTPLTSS"
BASE COUNT          597 a         1044 c          979 g          595 t
ORIGIN      
        1 aaaccggaat tcggaacatg ccgccgggcg accaacggtc gagccggcca aggcaacact
       61 gcggatttct cccgaatcgt acaccatagc ggcagacgat cccggtttgc aactcctcgc
      121 ggcccgaaac gagagcggta aggtccgaga tctgacctcc caggtggcat ggaccatcga
      181 accgccgggt atggccgagc tcgagccagg gggttatctg cggccggttg ggcccggcgt
      241 cgtcacggtc aaggctgcgc tcgaaggtca atcggtgacg gcgcggatca cgctcgaacc
      301 gcggtcgacg cggtcttggg atttcgccga ggatatcgta ccagtcttca cccggctcgg
      361 atgcaatacg ggtgcgtgtc acggcaaggc cgacggccag aacggctttc atctctcact
      421 cttcggctac gaccgagccg gcgattttca ggctctggca cgggacggcg ggcagcgccg
      481 gttatcgggt ctggttccgg aagcaagtct cttccttacc aaggccacgg gaacggtccc
      541 ccacgctggt ggtcggcgac tgaccgtagg ctcgccggag tatcaaactt tgctggcgtg
      601 ggtcaaagac ggggcaccgg aacgtcgcgg cacgacgcac ggaccggtcg tgaaggtggc
      661 ggtcgagccc ggctccgagc cttttgccga gccgggtctg aggcagctcc gtgtgacggc
      721 tcactacgaa gacggccacc agcgcgacgt gacccggctg gcgacttacc gcgtcaacga
      781 cgacgcggcc ggctccgtga cgccccaagg gcaggcggca ctcttgcgca ggtctgagac
      841 tgatttgatc gtccgctatc aatcgtatgt cttgaatacg cggctaggga ccgtcatcaa
      901 tcccgagctc gccttcgact ttggcaagct caggcggacc aactttgtcg atgacgagct
      961 cttcaagcga ctcgaggcgc tcaaggtacc accgagcccc cgcgccggcg acgccgcgtt
     1021 tctccgccgg gtttcgctcg acctgaccgg cgagcagccg gcacccgaag acgtccgccg
     1081 tttcctcgcc gatcatgatc ccgacaaacg tacgaaactc gtcgaccgat tgctcgccca
     1141 gcccgaattc gtggccttct ggcggatcaa gctaggcgac ctactccaga tcagctcggc
     1201 acggcacggc aacggggctt atcgctacca ggcgtggatc gatcgctgct tgaacaagaa
     1261 tgagccgtgg gacaaggttg taagtacgct tctgaccgcg gtcggcgatc ctaacgacat
     1321 tgagacaggc ggcccggtca actacgtgat ggacacgttc gagcccaacg tccaagccga
     1381 gcagacggct cagcgattcc tgggcctacg catccgctgt gcccagtgcc acgatcatcc
     1441 gtttgacgtg tggacgcagg atgcctacta cggcctggcg tcctttttcg ccaaggtgca
     1501 gcgcagcggt cgcggggcga tgatggggcg cccgaccgtc acgatcaatc ccgatggcaa
     1561 ggtcgttcat ctgcgcacca aacagccggt tgagccgcgg ctgctcgacg gcagggccgt
     1621 ggcggtgccg gcgaaagacg atccacgcaa ggtgctcgcc tcgtggatga cggcgccgga
     1681 gaacccttat ttcgcccgag cgaccgccaa ctgggtctgg tcgcagctct ttggcaaggg
     1741 actggtcgac ccgcccgacg atatgagccg cgcgaatccg gccgtccatc ccgaactgct
     1801 cgacgccctg gctcgccatt tcgtggccac caagttcgac ctgcgcgcct tggtccgcac
     1861 cattgccgtc tccgaatcct atggtttgtc gtcggccacg gtgccgggta acgagcgtga
     1921 tacgcgactt ttttctcacc aattgccacg ccctctgacg gcgcaccaga tggctgacgc
     1981 gctggcccag gccaccgacg tcgccaatcg atttcaaaac gtcgcccggc gagccatcga
     2041 cttacccgac ccgatgtcag cgagcacgat cctcgatacc ttcggccgtt gcgcccgaac
     2101 cacggcatgc gcgtcgacgc cggccccggc tctttcgctg cggcaggcct tgcttttgat
     2161 cggtggcgat gtcgtcgagg gcaaggtctc gaacttgaac ggctatctcg cgagcgcgct
     2221 caagctagag ctcgagccgg aagagctcgt cgaaaacctc tatttccgaa cggtgtgccg
     2281 gcctccgacg gccgaggaac tctcgcactg gagtgccgag ctcaaacagg ctgcctcaaa
     2341 gcgcgaggcg gcggaagacc tcttctgggc gctcttgaac tcacgggagt ttgcgtttaa
     2401 ccattgatcg cgcgcctggt gatcgcttag ttccgggata gctagaggac catccgagtc
     2461 tcgatccgaa agcttggctt ctgatacgaa agggaactga ccgatgtctt cgattccgag
     2521 ccggcaaccg tgtcgtggtc ccaaccgccg cgaggtcctt cggatcggtt cactcggctt
     2581 tctggggctg ggcctcgatg aatggctcag gttcacagcc agggccaact cgacgaaggc
     2641 cagcgattcc gtcaaggtga agaattgcat cttgatctgg ctggctggcg ggccgtcgca
     2701 catcgacacg tttgatccca agcccgaagc tggcgtcgac gttcggggag aattcaagcc
     2761 aatcgcaacc tcggtccccg gtttgcaaat tagcgaggtc ttccccaatc tcgccaaggt
     2821 catggatcgg gtggccctga tccggagcat gacctcgccc gaggctgacc acgaccgggc
     2881 atctcaccac atgcttaccg gctaccgccc gacgccggcc ctcgtgtatc cagggtacgg
     2941 cagcgtcgtg tcgaagaccc gcgaggcgac gcgcggtatg ctgccgccgt ttgtcgccat
     3001 tcccgacgcg ccgatctacg cttcgagcgg atttctgacg ccggcctacg acccgtttac
     3061 ggtgagcggc gatcccaacc aagagagctt ccgagttcaa aacctgactc cgccagacaa
     3121 actgactctc gaacggctac tgcgtcgccg cgacatggtc aggaagctgg acgatttcgc
     3181 ccacgacgtc cctggtacac cgttaacttc tagtc
//
LOCUS       DATAQL010001277         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3487808, whole genome shotgun sequence.
ACCESSION   DATAQL010001277 DATAQL010000000
VERSION     DATAQL010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487808"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VHS97_21980"
     CDS             <1..274
                     /locus_tag="VHS97_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450940.1"
                     /translation="RLLKTCYGLLDQAEQSVALLTGVDPAGNPLSAPFDASATIITRE
                     VVSAVTLSPEVESNPPKKRKPTATPPSPKKEPPAPTDPSGGVDPPF"
     gene            583..1557
                     /locus_tag="VHS97_21985"
     CDS             583..1557
                     /locus_tag="VHS97_21985"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX3450941.1"
                     /translation="MMSSPTASIESSALVEYLEGALRRVEEALSGYLPEIVAGEGAAC
                     PARLAMAMRYSVLGGGKRLRPVLCLMAAEACGGDAEMALPAACALEMVHTYSLIHDDL
                     PAMDDDDLRRGRSTCHRAFDEATAILAGDGLLTLAFEVVARDVKPPSAALQCVQILAE
                     AAGPSGMVAGQMADLQAEGRTDILGLPRNGGAKVETMATLAALESIHRRKTGALLRAP
                     LRMGAVIAAAPDSCVEALDRYGTSVGLAFQIVDDLLDVQGDETKLGKRVGKDSELGKW
                     TYPRFLGVDGSRRRARQLAEEAVAALGPFGPRGDRLKDLALALLERDR"
     gene            1557..3539
                     /gene="dxs"
                     /locus_tag="VHS97_21990"
     CDS             1557..3539
                     /gene="dxs"
                     /locus_tag="VHS97_21990"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX3450942.1"
                     /translation="MNHPDSVLGRIHSPADLKDLPETDLEQLAAEMRSELIDVVGRRS
                     AHFASNLGVVELCLALHLSFDFTRDRLIWDTGHQIYPHKLITGRAARLHTIRTKGGLM
                     GYPNPSESPFDLFMTGHAGCAPSTALGLKVGDELVGSPDRHAVAVIGDGALPSGIVFE
                     AFNHASGLRKKLLVILNDNKMSICPRVGGLADYLDRARMSSTYHDWNKRVKSLLPNIP
                     LVGEHAERLLHQFKDAIKASLRHGMLFEELGLVYLGPIDGHDLKTLRIYLERVKSLDE
                     PVLLHVMTNKGHGFEPAVKDPVKFHAPAPFQKTGNGVVPLKPSSSRVYTDAVSDTLYE
                     ACRRDSRIAVITAAMCEGNKLQKIRDTFPRQFFDVGICESHAVAFAAGMAKAGARPVV
                     DIYSTFLQRSYDQIFQEVALQNLPVVFCLDRAGLVGADGPTHHGAYDIGYLRVFPNMV
                     VMAPGDEKDIAPMFEFALNHSSPVALRYPRANLDTIERDVQPVELGQAEILEWETDGM
                     LLAFGAMVGSCLRVAERLHDRFGLRVGVVNARFIKPLDRVTITKAIEECGFILTVEEG
                     ALMGGFGSAVLEAANEAGLPTTHIRRIGIPDRYILHAERDEQLAEVGLDVEGITQAAL
                     ALARATGWTPPERETDTKAKTAINSAAAAERGILTG"
     gene            3576..4481
                     /locus_tag="VHS97_21995"
     CDS             3576..4481
                     /locus_tag="VHS97_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX3450943.1"
                     /translation="MGPADSTADPGPQPPRPLKLVILGNGIKPEVHILAKTLADAVRQ
                     APGIELTGVDLSADSNLSSLPADVAVVLGGDGTVLHAARRMEDHPTPVLGVNAGRLGF
                     LADLTPAAFQERLDDLAAQRFTIDNLMTMACTLTPRVGPTRAFRGLNDAVVRSAPVFH
                     LIDVGLSIDGESVMTYRGDGLIIATPVGSTAHSLSAGGPILPPNAHMFVVTPICPHTL
                     TQRPLVDSGHKVFEISPQGEGISTVLVIDGQVQIPLRSGDRVTIRRAKTPFPMVRLPG
                     HSFYRTLRDKLGWGALPTAATRSVI"
     gene            complement(4494..>5735)
                     /locus_tag="VHS97_22000"
     CDS             complement(4494..>5735)
                     /locus_tag="VHS97_22000"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX3450944.1"
                     /translation="IDPGGMIELPMEDIHGRFVFDDGKVTMNDVNFKFRGAPVKFSRG
                     NVFLEDSGQFDLNVHELCVDEIRFDLDLRKKMPLLMAQFALRLDDGRTFRAHGDLQIG
                     WSGVEGEPAWCKWWNTLVVFNDNSVRTGIPLEHIQGQLDNVSGWSNGAALEVKGAIRL
                     ASVSILGQQITEVESPFQISQGVARLIDVSGRYLKGEIQGDNCWITLDSTPHYNTNLS
                     IRGAQLEQYALTIPGRQSYRGNVAVKIELNGSGNDVRNLYGSGEAHITEGDLGELGPV
                     LRFATALNRVSNNLFSPPDRPRTPGKTAFDSADVAFTIARGHTTFDPIKFTGNAFSLQ
                     GRGTMNPQGNLDLRLSVLWGRDRLHIPLVSDFTREASTPLVIAHVWGTPSNPQFDIKP
                     LPLLDEFFKALGRGRAERRSR"
BASE COUNT         1094 a         1763 c         1756 g         1122 t
ORIGIN      
        1 gcggttgctc aaaacgtgct acggcctcct cgatcaggcg gagcaatccg tggcactctt
       61 gaccggggtc gaccccgcgg gcaatccgct ttccgctcca ttcgatgcga gcgccaccat
      121 catcacgcgg gaagtcgtct cggctgtgac actcagtcca gaggttgagt caaatcctcc
      181 caagaaacgg aaaccgacgg cgactccgcc ctccccgaaa aaggaaccgc cagcgccgac
      241 cgacccgtcc ggcggggtcg atccgccgtt ctgatcgctc ccgatgccac gaaattcgac
      301 ccgatgtcca tttagaacaa caagatggga gcatcgggca aaatatgcgg gctaagcgag
      361 cgcctttaac tttgtattgc aaaggatgta gggtgatggg tagactttca aggggcgaat
      421 gtcaacaata gcgaagcgta caagatggcc cgggtatgga agaccgataa gcatgcgttc
      481 ccaagccggg ggtgctgggc catcaggtcg tcgtttcgaa tacgccggct ttacccggtc
      541 aagttgccgg gaacgaatgg aaacggttga aacgattgaa ccatgatgag ttctcccacg
      601 gcatcgatcg aatcgtcagc gctagtcgag tatcttgaag gggcgttgcg gcgggtcgaa
      661 gaggccctga gcggctacct tccggagatt gttgcgggcg agggggcggc atgtccagcg
      721 cggctggcga tggcgatgcg gtacagtgtg ctgggggggg gtaagcggct gcgaccggtg
      781 ttgtgcctga tggcggccga ggcctgcggc ggcgatgcgg agatggcgtt gcccgcggcc
      841 tgtgcgctcg agatggtcca tacgtattcg ctgatacatg atgacttgcc ggctatggac
      901 gatgacgact tgaggcgtgg ccgttcgacg tgtcaccggg cgttcgacga ggccacggcc
      961 atcctggctg gcgatggtct tttgacgctt gctttcgagg tggtggcgcg cgatgtcaaa
     1021 ccgccgtcgg ccgcgctaca gtgcgttcaa atcctggccg aggcggcagg tccatcaggc
     1081 atggtcgccg gccagatggc cgaccttcag gccgaagggc gtaccgacat tctcggcttg
     1141 ccgcggaatg ggggcgccaa ggtcgagacg atggcgacgc tggcggctct cgaatcgatc
     1201 catcgacgta aaacaggggc gcttttgcgg gcccccttgc gtatgggcgc cgtgatcgcg
     1261 gcagcccccg attcgtgcgt cgaagcccta gaccgttacg gcacgtcggt gggattggct
     1321 tttcagatcg tcgatgacct gctcgatgtt cagggcgacg aaaccaagct gggcaagcgc
     1381 gttggtaagg attccgagtt gggcaaatgg acgtacccca ggtttctggg cgtcgacggc
     1441 agccgccgcc gcgcccggca gttggccgaa gaggcggtgg cggcgctcgg cccgttcggc
     1501 ccgcgtggcg accgactcaa ggacctggcg ctagctcttt tggaaaggga tcgttgatga
     1561 accatccgga ttcggtgctg gggcgaatcc actcgcccgc cgacctcaag gatctgcccg
     1621 aaaccgatct agaacagcta gccgctgaga tgcgatccga gttgatcgac gtggtcggcc
     1681 gccgctcggc tcatttcgcc agcaacctcg gagtcgtcga gctttgcctg gcgttgcatc
     1741 tgagttttga cttcacgcgc gaccggctga tctgggatac aggtcaccag atttatcctc
     1801 acaagttgat caccggacgg gctgcccgcc tgcacacgat tcggaccaag gggggcctta
     1861 tgggttaccc caatccgtcg gagagcccct tcgacctgtt catgacaggg cacgccggct
     1921 gcgcgccctc gacggccctg gggctcaagg tcggcgacga actcgtaggg tcccccgacc
     1981 ggcacgccgt tgccgtcatc ggtgacggcg cactcccctc ggggatcgtc ttcgaggcct
     2041 tcaatcatgc cagcggattg cggaaaaagc tgctggtcat cttgaatgat aacaagatgt
     2101 cgatctgccc gcgggtcggc ggcctcgccg attatctcga ccgcgcgcgg atgtcgagca
     2161 cctatcacga ctggaataag cgggtcaagt cactcctgcc caatatcccg ttggtcggag
     2221 agcacgccga gcgcttgctc caccaattca aagacgcgat caaggcctct ttgcgccacg
     2281 ggatgctctt cgaggaactc ggactcgtct acttggggcc gatcgacggc cacgatctga
     2341 agaccttgcg aatctatctc gagcgcgtca agtcgcttga cgagccggtg ttgcttcatg
     2401 tcatgaccaa caaaggccat ggatttgagc cggcggtgaa agacccggtc aagtttcatg
     2461 cccctgcccc ctttcaaaag accggcaacg gcgtcgttcc cttgaaaccc tcgtcgagcc
     2521 gtgtgtacac cgacgcggtc agtgataccc tttatgaggc gtgccgccgt gactctcgga
     2581 ttgccgtgat caccgccgca atgtgcgaag ggaacaaact gcagaagatt cgggatacat
     2641 tcccgcgcca attcttcgac gttggcatct gcgagagcca cgccgtcgca tttgccgccg
     2701 gtatggccaa ggccggagcg cggccggtgg tcgacattta cagcacgttc ctccagcgct
     2761 cctacgacca gatcttccag gaggtcgctc tccaaaactt gccggtcgtc ttctgcctcg
     2821 atcgcgccgg tttagtcggg gccgatggac cgactcatca tggcgcttac gacatcggtt
     2881 atttgcgcgt attccccaac atggtcgtca tggccccagg cgacgagaaa gacatcgcgc
     2941 ccatgttcga gttcgccctg aaccactcat cgccggtagc gctgcgttac ccgcgggcca
     3001 acctcgacac catcgaacgc gacgttcagc ccgtcgagct ggggcaggca gagatcttgg
     3061 agtgggagac cgacggcatg ctcttggcct tcggcgcaat ggtcggtagc tgcttgcggg
     3121 tggccgagcg gctgcacgat cgctttggcc tgcgcgttgg tgtggtgaat gcacggttca
     3181 taaagccgct cgatcgcgtg acgatcacca aggcgattga agagtgtggt ttcattctga
     3241 cagtggagga aggggctctg atgggcggct ttggctcggc agtgctcgag gcagccaacg
     3301 aggcgggcct tcccactacc cacattcgcc gtatcggcat ccccgatcgc tacatactcc
     3361 acgccgagcg tgatgaacag ctggcggagg tcggcctcga tgtcgaagga atcacccagg
     3421 cggcgctagc gctggcgcgg gccaccggct ggactccacc cgagcgcgag actgacacca
     3481 aggccaagac cgccatcaac tctgccgccg ccgccgaacg aggaatcctc accgggtagg
     3541 aaccgttctg cgcgcgctaa agagacgagt acgtcatggg acccgctgac tccacggctg
     3601 atccaggacc gcaaccaccg cggccgctca agctggtcat cctgggcaac ggcatcaagc
     3661 ccgaggtcca tattctggcc aagacactgg ccgacgccgt tcggcaggcc cctgggatcg
     3721 agctgaccgg cgtcgatctc tcggccgatt ccaacctgtc gtccttgccc gccgacgtgg
     3781 cggtcgtgct gggaggagac ggcaccgtcc tccacgcggc gcggcggatg gaggatcatc
     3841 cgacccccgt gttgggcgtt aacgccggcc ggctcggctt cctggccgac ctgacgcccg
     3901 ccgcatttca agaacggctt gacgatctgg ccgctcagag gtttacgatc gataacctga
     3961 tgaccatggc gtgcacactc acaccccgag tcggacccac ccgcgcgttc cgtggtttga
     4021 acgacgcggt cgtgcgttcg gccccagttt tccacttgat cgacgtcggc ctgtcaatcg
     4081 acggtgaaag cgtgatgacc tatcgcggcg atggcctgat catcgccact cccgtcggtt
     4141 cgacagccca cagcctctcg gcagggggac cgatactacc ccctaacgcc cacatgttcg
     4201 tggtcacccc gatctgcccg catacactga cccagcgacc gctcgttgac agcgggcaca
     4261 aggtcttcga gatttcacct cagggcgaag gtatctccac agtgctagtc atcgacggcc
     4321 aagtgcagat cccactccgt tctggtgacc gtgtcacaat ccgccgtgcc aagactccct
     4381 ttcccatggt ccggctcccc ggtcacagtt tctatcggac actgcgcgac aaactcggtt
     4441 ggggggcatt gccgacggcc gcgaccaggt cggtcatttg acccgtcgtt ttctcaacgc
     4501 gatcggcgtt cggcgcggcc tcttcccaga gccttgaaaa actcgtccag gagaggcagc
     4561 ggcttgatgt cgaattgcgg gttcgacggt gttccccaca cgtgggcaat cacgagagga
     4621 gtactcgcct cgcgcgtgaa gtcgctcacg agcggaatat gcaggcggtc tcgtccccac
     4681 aaaacgctga gccggagatc aagattgcct tgcgggttca tggttccccg accctgcagg
     4741 ctgaaggcgt tgccagtgaa cttgatcgga tcgaaagtcg tatgtccgcg agcgattgta
     4801 aacgcgacgt cggccgaatc aaacgccgtc tttcccggag tgcgtggcct gtctggcggc
     4861 gaaaagagat tattggaaac ccgattgagc gcggtggcaa accgaagcac cgggcccagc
     4921 tcgcctaaat cgccctcggt gatgtgggcc tcgccgctgc cgtacaggtt acgcacgtcg
     4981 ttgcccgagc cgttaagctc gatcttgacg gcgacattcc ctcggtacga ttggcgtccc
     5041 gggatcgtaa gcgcgtattg ctcgagctga gcaccgcgaa tcgacagatt cgtgttgtaa
     5101 tggggcgtcg aatcgagggt aatccagcaa ttgtctcctt ggatctcgcc cttcaaatag
     5161 cggccactta catcgatgag ccgtgccacc ccctggctga tttgaaatgg cgactcgacc
     5221 tcggtgatct gctgtccgag gatgctgaca ctggcgagtc taatcgcccc tttaacctca
     5281 agcgccgcac cgttcgacca gccgctgacg ttatcaagct gcccttgaat gtgttcaagc
     5341 gggatgccgg tcctgaccga attgtcgtta aacacgacga gggtgttcca ccacttacac
     5401 cacgccggct cgccctctac acccgaccaa ccgatctgaa ggtctccgtg ggctcgaaac
     5461 gtgcggccat cgtcaagtcg gagggcaaac tgcgccatca ggagaggcat tttcttgcgt
     5521 agatcgaggt cgaagcgaat ctcgtcgaca cacagttcgt ggacgttcag gtcgaattgg
     5581 cccgagtcct cgagaaagac gttcccacgg gagaacttga ccggcgcgcc gcggaacttg
     5641 aaattcacgt cgttcatggt gaccttgccg tcatcgaaga cgaaccggcc gtgaatgtcc
     5701 tccatgggca gctcgatcat accgccaggg tcgat
//
LOCUS       DATAQL010001278         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2878679, whole genome shotgun sequence.
ACCESSION   DATAQL010001278 DATAQL010000000
VERSION     DATAQL010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2878679"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..1623)
                     /locus_tag="VHS97_22005"
     CDS             complement(319..1623)
                     /locus_tag="VHS97_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3450945.1"
                     /translation="MNQQTFSRRTFLRGVGVSMALPWMESLRVWGDEAKAVRGGSEAP
                     VRLAVLFAGNGFHSKEWWARGAGRSMELGQVLAPLADFRDRMLFIRGLYNAEALKGNI
                     HSSQTGNLLSGAPLASGGEIRSGMSFDQLLARTHGKSTKVPSLVLACEKSNPSVHKNY
                     SMLYSSHISWTSPTAPSPLELYPALAFDRLFRDEAERGDKSVLDAVLADARDFRRVVS
                     SADQRKLDEYLDSVREVEQRIARAGQRGELQGWRPTLEVPNIARPADGIPQDIGEHMR
                     LMCDILVLGFQTDTTRICSLKLNNDHSSLRFPNLGVDYMIHHLLSHSDTADWLKVNQF
                     FLKQVAYIVRKLDLVREGNKTALDNSMIMFCSSMLNGNHDASQLPVVVLGGAGGRIDG
                     GRVLDYRDKPNRKMCSLYLSLLDKAGLHLDEFGDSRERLADV"
     gene            complement(1692..>4281)
                     /locus_tag="VHS97_22010"
     CDS             complement(1692..>4281)
                     /locus_tag="VHS97_22010"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3450946.1"
                     /translation="PGNLKPHSVAMHPSPSLRVAAGWKSPVSAILRVEGRVQHAHPEC
                     GNGVVWSLELRRGAVRQRLATGVSQGAKEIIAGPVQDLAVQPGDLVSLLIGPRDGNHA
                     CDLTAVDLTLAGGGHEWNLAREVSPNILADNPHADADGKFGVWHFYAEPDKQGPSESV
                     IPAGSLLARWQRAASADEKVRLAQALEALLKSGPPAAKDSPDGLLYRQLSSLRGPLVS
                     AVQHHVPANGPSSGVGVANATAAGLDPALFGKHPSGPVIDAASLCVQAPKVVEIRLPS
                     DLVEGCEFVTGGTLDTTTGAEGSVQLQLLSSRSEPAREVSPDLPIVVRDGSAARRRFE
                     TAFDIMRDVFPPALCYSKIVPVDEAVTLTLFHREDENLRRLMLDPAEAAELDRLWAEL
                     HFVSFDSLTLVDAFQQLLEYASQDGDPRVFEPMRKPITDRAAAFRRELVAAEPRHLDA
                     IVALADRAFRRPLSGNEASELRALYHKLRADDIPHDEAVRLVLARVLVSPAFLYRMES
                     APPGDKSAPVSDYELASRLSYFLWSSMPDAPLREAAASGRLQRPDVLAAQARRMAKDL
                     RVRRLATEFACQWLHIYDFDSLDEKSTRHFPTFAGVRSAMQEEAILFFADLFQNDGSV
                     LSIVDGDHTFLNEALAKHYGIPGVTGSDWRRVDGVKRYGRGGILGLAATLAKQSGASR
                     TSPILRGNWVAEVLLGDKLPRPPKNVPPLPDDEAATQGLTMRQLVERHTTDPRCASCH
                     VRIDAFGYALEAYDAIGRRRDRDLGDRPIDTSARLRDGTTFDGFDGLRDYLATTRREA
                     VIKQFCRKFLGYALGRAVQLSDDPLLQEMEQKLRNNGYRFSAILEAVVGSRQFREIRG
                     RDVTGS"
BASE COUNT          815 a         1340 c         1348 g          778 t
ORIGIN      
        1 tcccacgcgg agcgtgggaa cgagtctggc taccgcgaac gtccaggatc cgtcgctaga
       61 atcaaatgca ctgattaaga aatgtgtcga taacttactg tagaggggct gcaaagcagt
      121 ccggactctg cgcgtattga aaggacgcgg ccggactaag aaagttgccg gctgcctccg
      181 tactgagacg ttctttcgcc atgcacatct cgtcgttggt tccttcgccc aacttgacca
      241 gctttgccgg cttgctctgg gttggattgg ttggagaccc tcactcgaag ccgacgcgat
      301 cccctccacg tcaccgtgtc aaacgtccgc cagccgttcg cgtgagtcgc cgaattcgtc
      361 cagatgcaaa ccggccttgt caagcagtga aaggtacagg ctgcacatct tgcgattggg
      421 tttgtctctg tagtcgagca cgcggcctcc gtcgatgcga ccgccggccc ctcccaggac
      481 gacgaccggc agttgactgg cgtcgtggtt accgttgagc atactggaac agaacatgat
      541 catcgagtta tccagggccg tcttgttccc ttcacggaca aggtcgagct tacgcacgat
      601 gtaggcgacc tgcttcaaga agaactgatt gaccttcagc cagtcggccg tgtcggagtg
      661 cgacagcagg tgatggatca tgtagtcgac accgagattg ggaaatcgca gcgagctgtg
      721 atcgttgttg agcttgagcg agcaaatgcg cgtggtgtcg gtctggaagc cgagcacgag
      781 gatgtcgcac atcaggcgca tgtgctcgcc aatgtcttga ggaatcccat cggcgggtcg
      841 cgcaatattg ggcacttcta gagttggccg ccagccctgc agctcgccgc gctggccggc
      901 acgcgcaatt cgttgctcga cttcgcgcac cgagtccaga tactcgtcga gcttgcgctg
      961 atcggccgag ctcacaacac ggcggaagtc gcgggcgtcg gccagcacgg catcgagaac
     1021 gctcttgtcg ccccgctcgg cctcgtcacg gaaaagccga tcgaaagcga gcgccgggta
     1081 aagctccagt ggcgacggcg ccgtcggcga ggtccaggaa atgtgcgagc tgtatagcat
     1141 cgagtaattc ttatgcaccg acggattcga cttttcgcag gcgagtacca aactcggaac
     1201 cttcgtcgac ttgccgtggg tgcgcgccaa gagctggtcg aagctcattc ccgagcggat
     1261 ctcgcccccg gacgcgagag gggcgccgga aagcaggttt cccgtctgcg agctgtggat
     1321 gtttcccttc aaggcttcgg cgttgtagag cccgcggatg aagagcattc ggtcgcgaaa
     1381 atcggcgagc ggggcaagca cctggcccag ctccatgctc cggccggctc cgcgcgccca
     1441 ccattccttg ctatgaaaac cattgccggc aaagagaacc gccagccgca ccggcgcctc
     1501 gctgccaccg cggacagcct tggcctcgtc gccccagacg cggagcgact ccatccacgg
     1561 tagcgccatg ctcacgccga cgccgcggag aaaagtgcgg cgggagaagg tctgctggtt
     1621 catcgtttgc ctcgcggaac gtagtgcctt acagtctttt atactatcct gttcgacgac
     1681 agtcacgcgg atcaagagcc cgtcacgtcc ctgccgcgta tctcgcggaa ctgacggctg
     1741 ccgacgacag cctcgagaat cgccgagaat cgatagccgt tattcctgag cttttgctcc
     1801 atctcctgca gtaggggatc gtcggagagc tggactgcgc gacccagcgc gtagcccaaa
     1861 aacttgcggc aaaactgttt gatcacggcc tcgcgccgtg tcgtcgccag gtaatcgcgc
     1921 aggccgtcga agccgtcgaa ggtggttccg tcgcgcagcc gcgcggacgt gtcgatcggt
     1981 cgatcgccga gatcacgatc gcggcggcgg ccgatagcgt cgtaagcttc cagcgcataa
     2041 ccaaacgcgt cgatgcgcac atggcaacta gcacagcgtg ggtcggtcgt gtgacgttcg
     2101 acgagctgtc gcatcgtgag cccttgcgtg gcggcctcgt cgtcggggag tggcggcacg
     2161 ttcttcgggg ggcggggcaa cttgtccccc agcagtactt ccgccaccca gttccctctc
     2221 agaatcgggc tcgtacggga ggcgcccgat tgctttgcga gagtcgctgc caggcccaga
     2281 attccgcccc gcccataccg tttgacgccg tcgacacggc gccaatccga gccagtgacg
     2341 cccgggattc catagtgctt ggccagcgct tcgttcagga atgtatggtc cccgtcgaca
     2401 atcgagagca ccgaaccgtc attttgaaac agatcggcga agaagaggat cgcctcttcc
     2461 tgcatggcgc ttcggacccc ggcaaatgtc gggaagtgtc tggtgctttt ctcatcgagg
     2521 gaatcgaaat cgtaaatgtg cagccactgg caggcaaact cagtcgccag ccggcggacg
     2581 cgcaggtctt ttgccatccg acgcgcctga gcagccagga catcgggccg ttgcagccga
     2641 ccggaagcgg ccgcttcgcg cagcggtgca tcgggcatcg acgaccagag gaaatagctg
     2701 aggcggctcg ccagctcgta gtcagatacg ggcgctgact tgtctcccgg cggggcgctt
     2761 tccatccgat agagaaacgc aggcgaaacg agcacccttg ccagcaccag cctgaccgcc
     2821 tcgtcgtgag ggatgtcatc ggcgcgcagc ttgtgataga gagcacggag ctcgctggcc
     2881 tcgttgcccg agagcgggcg gcgaaaagcg cgatccgcca gggctactat tgcgtcgaga
     2941 tgacgcggct cggccgccac gagctcgcga cggaacgccg cggcccgatc ggtgatcggc
     3001 ttacgcatcg gctcaaatac acggggatca ccgtcctggc tggcatattc caaaagctgc
     3061 tgaaacgcgt ccaccagcgt gagcgagtcg aagcttacga agtgcagctc ggcccagagg
     3121 cggtcgagct cggcggcttc ggccggatcg agcatcagcc ggcgcaggtt ctcgtcctcg
     3181 cggtgaaaga gggtcagtgt gacggcttcg tccaccggga cgatcttgct atagcatagg
     3241 gcagggggga agacatcgcg catgatgtcg aacgcggttt caaaccgccg ccgcgcagcc
     3301 gaaccgtcgc ggacgacgat cggcaaatcg ggcgaaactt cccgcgccgg ctcggaccga
     3361 cttgagagca actgcagctg cacgctaccc tcggcgccgg ttgttgtatc cagagtgcca
     3421 ccggtcacaa actcacaacc ctcgacgagg tccgacggca accgaatctc cacgactttg
     3481 ggcgcctgta cacagagact cgccgcgtcg atgacggggc cgctggggtg cttgccaaag
     3541 agggcagggt cgagccccgc cgccgtcgca tttgcaacac cgactccgct cgagggtccg
     3601 tttgcaggca catgatgctg caccgcactg acgagcggtc cgcgcagcga cgagagctgc
     3661 cggtaaagca gcccgtctgg actgtccttg gcggcgggcg gaccagactt gagcaaagct
     3721 tcaagagcct gcgccagccg aactttctca tccgcgctcg ccgccctttg ccatcgggcg
     3781 agcaatgagc cggccggaat caccgactcg ctcgggcctt gcttgtctgg ctcggcgtaa
     3841 aaatgccaaa cgccgaattt gccgtccgcg tcggcgtgcg ggttatcagc caggatgttc
     3901 ggtgagacct cgcgtgcaag gttccactcg tgaccgccgc cggcgagtgt gaggtctact
     3961 gcggtgaggt cgcacgcgtg attgccgtcg cgcgggccga tcaaaagcga gaccaggtcg
     4021 ccaggctgga cggccagatc ctgtaccgga cccgcgatga tctctttcgc cccctgggag
     4081 acgccggtgg cgagcctttg ccggaccgcg ccccgccgca gctcgagcga ccagacaacg
     4141 ccgttgccgc attcagggtg agcatgctgc acacgcccct caacgcgaag gatcgcgctg
     4201 accgggcttt tccagccggc cgccacgcgc agggatggcg aaggatgcat cgccacgctg
     4261 tgtggcttca agttgccggg g
//
LOCUS       DATAQL010001279         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3795169, whole genome shotgun sequence.
ACCESSION   DATAQL010001279 DATAQL010000000
VERSION     DATAQL010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3795169"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..1838)
                     /locus_tag="VHS97_22015"
     CDS             complement(396..1838)
                     /locus_tag="VHS97_22015"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3450947.1"
                     /translation="MILPPDDPADLELPPEVMRAMGQAALERVVDFIASLPYQGACGD
                     IHAEELCRALRESAPEHGVPISELLDRLFHDWIPRSFNTAGPGYLAYIPGGGIFPAAL
                     ADLIADGTNRYTGIWQAAPALVQLEANVLDWFRDWMGFPSTTRGLFTTGGSMSVFNAV
                     LCARERQLGTEIRSGVLYTSNQAHAAVVKSAKLAGIFPDRVRLVSVDSQFRMRFDDLG
                     DQIARDQRAGLRPFLVVSTAGSTNTGAVDPLEAIADLCEREGLWHHVDGAYGAFFHLV
                     EPLRPLLAGLSRADSLTLDPHKGLFLPYGTGALLVRDGAALRNVHQVHASYMPPAVDQ
                     EFYDPAQYGPELSRGFPGLRVWLTIKTFGAARIRAAIAEKRALAVAAAERLAGVPGIN
                     LVAPPQLSLLAFCVQPTGSSLLEQNNATSRLLERVNARRRVMLTGCTVDGRFLARLCV
                     MSFRTRERHVELAVDQIIEEAAGILAEGVR"
     gene            complement(1977..3632)
                     /locus_tag="VHS97_22020"
     CDS             complement(1977..3632)
                     /locus_tag="VHS97_22020"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX3450948.1"
                     /translation="MNSPHPLGPRLELIRGGRSERIYELKGSDLHIGRIQGLDVYLDD
                     VRVSRHHARVEHRPDGTAYLVDLDSKGCTHLDGRRLTPFQAVPLRNGSRIRIVDYELI
                     FHDQAVELNDSGDGDSTILESLDNLSTEHLARRSVEPAQALKAILEINRAVGGGAELN
                     EVLNRALEGLMAVFPAAERGFILIVGPEGRPRLRALRQQDRGTQLPVLSRTILTQVVS
                     AGKAILINDTTIDPRFMRTKSVVSSFRTAMCVPLLGHDGRPLGMIQLDRRTGRKGFKA
                     GDLDLLAALSVPVGVAVENHHLLRERASWAAAREIQLALLPHGRPTIAGYAFWECYRP
                     TLEVGGDLYDYIAVEPSEPGEEGRARWAVTVGDVAGKGMAAALVVAGLCPEVRHSVRA
                     GAGPAEVLAGVNHRLCDQGVGGRFVTMALAVIDARSNVMTVANAGHLDPLVRRTSGTI
                     ESVGRSGAGLPLGVSAEAVYRPVSFNLEPGDLVVLYTDGVNESMNLDRQPFGEARVKE
                     VLAATKGGVGAAGEAILAAVRDHSAGRSQFDDITIVCFQRQPN"
     gene            3956..>4170
                     /locus_tag="VHS97_22025"
     CDS             3956..>4170
                     /locus_tag="VHS97_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450949.1"
                     /translation="MTRRHWLLLLAGLVVGLLISGRFALTAQDRAETPQAKSSHSPRE
                     KTGESAKLPALSADASDPLLRPLVVQG"
BASE COUNT          783 a         1341 c         1319 g          727 t
ORIGIN      
        1 gtgggacttt ttggaacacc acgcttgacg ctcgaaagga acctccgact attgtcagac
       61 taagtactac aatagtcata cgtcggccgg atgtcaaagc gctggtggtt cgcgggctgg
      121 ttttggcgat tatgcccatc aatctagtag ggctttccgc ctcaggcata agcggttgtc
      181 gtcggccgtg ctgcataagt ggcactccgg cgaagtgctc cagctcccgg aaccggaacg
      241 agtaggctgg tgccgatccg cgagaaatcg cggcgaggcc caccgacccg ccgtgactct
      301 cggtgggcct cacgatcctt tggatcgttc ggcgccaccc tacgaccgtc cgcggcgccg
      361 ccacgtcagg ggctatttca aagagcacat tcaagttatc tcacgccctc ggcgaggatc
      421 cctgctgcct cctcgatgat ctgatccacg gccagctcaa catgacgctc gcgtgtgcgg
      481 aagctcatca cgcacagccg ggccagaaat cgtccatcga cggtacagcc ggtgagcatc
      541 actcgccgcc gcgcgttgac ccgctcgagc agccgggagg tcgcattgtt ctgttcgagc
      601 agcgacgaac cagttggctg cacgcaaaag gccagaagcg aaagctgcgg gggcgcgacc
      661 aggttgatcc cgggtacgcc cgcgagccgc tcggcggccg caaccgccag cgcccgcttc
      721 tcggcgatgg cggcccggat ccgggcggcg ccgaatgtct tgatcgtcag ccagacccgc
      781 agtcccggga aaccacgcga cagctcgggg ccgtactgag cggggtcgta gaattcttgg
      841 tcgacggccg gcggcatgta gctggcatgc acctgatgaa cgttccgcag ggccgcgccg
      901 tcgcggacca agagcgctcc ggtaccgtac ggcaagaaaa gccccttgtg cggatcgagc
      961 gtcagcgagt cggcccgcga cagacctgcc agaagtgggc gcaggggctc gaccagatgg
     1021 aaaaacgcac cataggcgcc gtcgacgtgg tgccatagtc cctcgcgttc gcagagatcg
     1081 gcgattgcct ccagcggatc gacggcgccg gtgttcgtcg atcctgccgt cgacaccacc
     1141 agaaacggcc gcagacccgc gcgttggtct cgcgcgattt gatcgcccag atcgtcaaac
     1201 cgcatgcgga actgtgagtc gaccgatacc aggcggaccc gatcgggaaa gatgccggcc
     1261 agcttggccg acttgacgac cgccgcatgg gcctggttcg acgtgtaaag gacgcccgat
     1321 cggatctcgg tccctaactg ccgctcgcgg gcgcagagta ctgcgttgaa gacggacatc
     1381 gagccgccgg tcgtgaacag gcctcgggtg gtcgatggga agcccatcca gtcgcgaaac
     1441 caatcgagca cgttcgcctc gagctggacc aacgccggcg cggcctgcca aatgccggta
     1501 tagcgattcg tgccgtcggc aatcaggtcc gccagcgcgg ccgggaaaat gcccccgccg
     1561 ggaatatagg ccaggtatcc cggaccggcc gtgttgaacg agcgggggat ccaatcgtga
     1621 aagagccggt cgagcagctc cgaaatcggt acgccatgtt cgggcgcgct ttcgcggagc
     1681 gcacggcata attcctcggc gtggatatca ccgcaggccc cctggtatgg gagtgacgcg
     1741 atgaaatcca cgacccgctc cagcgcggcc tggcccatgg cgcgcattac ttcgggtggc
     1801 agctccaagt ccgcgggatc gtcgggcggc aagatcacta tcgggtgcac tccgtttcgg
     1861 gtgaagttgt cggctttgag cgaagattgt atgacaaagg cgggcgcccg gcgatcgtaa
     1921 gccagttcag cggcctctaa gaatcgcgcc acaacaacgc gaatgcgcgg gcgagttcaa
     1981 ttcggctggc gctggaagca gacgatggta atgtcgtcga attgcgagcg gccggcggaa
     2041 tggtcgcgga cggccgccag gatggcctcg ccggccgcgc cgactccgcc ctttgttgcg
     2101 gccaaaacct ccttcacccg cgcttcgcca aacggctgac ggtcgagatt cattgactcg
     2161 ttgacaccgt cggtgtagag gacgaccagg tcgcccggct cgagattgaa ggagaccggc
     2221 cgatagacgg cctccgccga cacacccagc ggcaggccgg ccccggagcg gcccacggac
     2281 tcgatcgtgc ccgaagtccg gcgcaccagg gggtccaagt gcccggcatt cgccacggtc
     2341 atgacgttcg accgagcgtc aataaccgcc aaagccatgg taacaaaccg cccaccaacc
     2401 ccctggtcgc agagccggtg gtttacgccg gcgaggactt ccgccggtcc ggctccggcg
     2461 cgcaccgagt gtcgcacctc cggacagagg cctgccacga cgagcgcagc ggccattcct
     2521 ttgccggcga catcaccaac cgtcaccgcc caccgcgctc ttccttcttc gcccggttcc
     2581 gagggttcca ccgcgatata atcgtaaagg tcgccgccga cttcgagagt tggccgatag
     2641 cactcccaga atgcatagcc ggcgatcgtg ggccggccgt ggggcaaaag tgccagctgg
     2701 atttcacgcg ccgccgccca tgaggcccgc tccctgagca aatgatggtt ttcgaccgcc
     2761 acgcccaccg gcaccgacag tgcggcgagc aagtcgaggt cacccgcctt gaagcctttt
     2821 cggccagtcc tccggtcgag ctggatcatg cctagcggcc ggccgtcgtg ccccaagagc
     2881 ggcacgcaca ttgccgtgcg aaacgaagag acgacgctct tggtacgcat gaaccgaggg
     2941 tcgatcgtcg tgtcgttgat gagaatcgct ttgccggcgc tgacgacctg ggtaaggatt
     3001 gtgcgagaaa gcaccggcag ctgcgttccc cggtcctgtt ggcggagcgc gcgcagccgc
     3061 ggtctcccct cgggccccac gatcaggatg aacccacgct ccgcagcggg gaaaaccgcc
     3121 atcaacccct ctagggcgcg attgagaact tcattgagct cggccccacc gccgaccgct
     3181 cgattgatct cgagaatggc cttgagtgct tgcgcgggct cgaccgagcg gcgtgcaagg
     3241 tgctcagtgc tcaagttatc gagactctcg agaatcgtcg agtccccgtc ccccgagtcg
     3301 ttgagctcga ccgcctggtc gtgaaagatc agctcgtagt cgacgatcct gatccggctg
     3361 ccgttccgga gcggcacggc ctggaatggc gtcagtcggc ggccgtcgag atgggtgcat
     3421 cccttgctgt cgagatccac aaggtaagcc gtgccgtccg gccggtgctc gacccgcgcg
     3481 tgatgacgtg atactcggac gtcgtcgagg taaacatcca ggccctggat ccggccgatg
     3541 tgcaggtcag accctttcag ttcataaatt cgctcggatc gcccgccccg gataagttcg
     3601 agtctggggc ccagcggatg tggactgttc atgatgttgc ctgcgcgagg gaagccgcca
     3661 agcccacccg ttgtgggtca cagctctcgt agcaccagtt cgtgcctaaa cttgttcgtt
     3721 tgccatggtc tcgaacgacc attctacttg accgctggct cgagtgcctc cctgtgaaag
     3781 attaggcgtg gttgagctga tcgaggtcaa cgtttcggcc gcaagtcccg ctggttggtc
     3841 cgagacgtcg cggttacaat gggccataat gttccagtaa aaggggaatg accggcgatt
     3901 ttgcgcctgc aggcgcccat tccgcctcgc gcctcgtgtc taaggggagc ggtgcatgac
     3961 acgaagacac tggttactgc tgctggcagg cctggttgtc gggctcttga tctcggggcg
     4021 attcgcgctc acggcccagg accgggccga gaccccacaa gcaaaaagta gccactcacc
     4081 cagggagaaa accggcgaat ccgccaaatt gccggcactg tcggccgatg cgagcgatcc
     4141 gctcttgcgg ccgctcgtcg tccagggtaa
//
LOCUS       DATAQL010001280         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_892089, whole genome shotgun sequence.
ACCESSION   DATAQL010001280 DATAQL010000000
VERSION     DATAQL010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892089"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VHS97_22030"
     CDS             complement(<1..252)
                     /locus_tag="VHS97_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450950.1"
                     /translation="MPSALVRRAACALWLALGTFTQAQTTGTRAPAENDNAEGSPPTS
                     AGPAAEPEEPPKSDEATPSSPPKTLLERFQRLLSPKEDPD"
     gene            complement(569..2323)
                     /locus_tag="VHS97_22035"
     CDS             complement(569..2323)
                     /locus_tag="VHS97_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450951.1"
                     /translation="MSLAIPDPNRYTIPPIKAIQTARALRNLRWCLTRNTIRSLLAGS
                     RLRVAMILFCSVVFWVGLLGLFLGGFELIGLSDELFQAIFEYLFSMFFLSISIMLFFS
                     TGIITYTALFHSREGAFLLSTPASTDRIFAHKFAEAVAISSWGFFLLGSPLMAAYGLT
                     VKAPAAFYVMFVIYLFMFVLMAGSLGATTAIVIANIFPKQQKLVLSLAVIATVAVVVG
                     VAVRLWQTPGDTLSSDWLVNMQNRLAFCQHPLWPSRWMSAGLLASARADWSRSGYYLM
                     ILSAHAGLLYLAAAAVARDLYFRGYSRVQGGRSNRRHTGLYAVDAVFHRLFFFLPRPI
                     RLLILKDLRTFLRDPAQWSQFLIFFGLLAFYFLNIPRLGYGVQSPSWRNLVSFLNISV
                     TALILSTFTSRFIFPLLSLEGRNFWVLGLLPVGRAQILWGKFAFSVGISLVATEGLVF
                     LSDLMLGMSPVMIALHVGMVAILCMGLSGISVGLGARLPNLRETDPSKIAVGFGGTLN
                     LLVSLVFIFAIVTALAVPCHLYFLGQEHAETTTLALSHEGFRFWLTVAIVVSLIIGAV
                     GTIVPLRIGIKAFRHMEF"
     gene            complement(2320..3087)
                     /locus_tag="VHS97_22040"
     CDS             complement(2320..3087)
                     /locus_tag="VHS97_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3450952.1"
                     /translation="MIALSGVTKRFGSKCAVDGLDLEVRPGELFAFLGPNGAGKTTTI
                     KMICGLLAPTSGTVRVGGFDASSQQARQLLGYVPDQPYLYDKLTGREFLRFVVEMYGL
                     EPRRAAQRIDELIETFEMGDFVDELCENYSQGMKQRVVFASALVHDPKVLVVDEPLVG
                     LDPRSARVVKDLFISQARSGCAVLMSTHLLAIAEELADRIGIVDHGRMLALGTLEQLR
                     GQVQHRGPLEDLFLKLTGDHRLATNGVVSSELAELQP"
     gene            complement(3084..>3669)
                     /locus_tag="VHS97_22045"
     CDS             complement(3084..>3669)
                     /locus_tag="VHS97_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450953.1"
                     /translation="LLKGNPHLGRSPSFRLRVESVYRVDSTGNLNSFNLDVKSRDSTE
                     TLIAVKGELKEKKMEIVSRGPVEFFNRKMSIDYLPRSVVQDVLGPLDRLPGLHVGQKW
                     KSQVINPFTARVEDVRVEVARKDLIHWNGDAVRAFEVVQKASLSGISLRTWVNFDGVI
                     LRQEVPTPFVRLMMERRADVDEEISKLPTTVPGP"
BASE COUNT          847 a         1076 c         1099 g          647 t
ORIGIN      
        1 atcgggatct tcttttggcg agagcaacct ctgaaagcgc tcaagcagag ttttcggcgg
       61 agagctcgga gtggcctcat cggatttagg gggctcttcc ggctcggcgg ctggaccggc
      121 agacgtcggt ggtgacccct cggcgttgtc gttttccgcc ggcgcgcgcg ttcccgtcgt
      181 ttgggcttgg gtaaaagtcc cgagagccag ccacaacgca caggcagctc ttcgaaccag
      241 cgcggaaggc atccacggtc tctgtcttcc aggggcgacc gattccaagc gcaccgcgct
      301 aagtcaatgt agatgacacg cccctcattc cgtcggtcgt ctccgggcga aacttgagcg
      361 aattggaagg accgtcggcg cgaagacgcc tcaaattgtg gacgcagcgc gtcttgcttg
      421 ggggccgggg gatggcttgg gcatcctaag tggacccgcc gaagtcgccg cgcagcgtag
      481 gtcgggcttt ctagcccgac gttttccttg aaggcacaaa atgtcaggct ggaaagcctg
      541 agctacacaa gtagttctgc ggtctcactt agaattccat gtggcgaaag gccttgatgc
      601 caatccgcag gggaacgatc gtaccgaccg ccccgatgat caagctgaca acgatcgcca
      661 cagtcagcca gaatcggaac ccttcatgcg agagggccag ggtcgtggtt tcggcatgct
      721 cctgaccgag gaaataaaga tgacagggga ccgccagggc cgtcacgatc gcgaaaatga
      781 agacgaggct gaccagcaag ttgagcgtac caccaaatcc gacggcgatc ttcgatggat
      841 ctgtctcgcg caggttaggg aggcgggcac ctaagccgac gctgatcccc gagagtccca
      901 tgcagaggat ggccaccatc ccaacatgga gcgcgatcat gaccgggctc attcccaaca
      961 tcaggtcgct caggaatacg agaccctcgg tcgcaaccag cgaaatcccc accgagaagg
     1021 caaacttgcc ccagagaatc tgggcccggc ccactggaag caggccaaga acccaaaagt
     1081 tacgcccttc cagcgaaagg agcgggaaga taaaccggct ggtgaaggtc gaaaggatga
     1141 gggcggtcac cgagatgttg agaaagctga caaggttgcg ccacgacggg ctctggacgc
     1201 cgtaacccag ccggggaatg ttaaggaaat agaaggcgag caaaccgaag aagatcagga
     1261 attgcgacca ctgcgctgga tcgcggagga aagtgcgcag gtctttcaag atgagaagtc
     1321 ggatcggccg gggcaagaag aagaaaaggc ggtgaaagac tgcgtcgacc gcgtagagcc
     1381 ccgtatgacg tcggttgctc cggccgccct gcacgcggct gtatccgcgg aagtacaggt
     1441 cgcgcgccac ggcagccgcg gccaggtaaa gaagacccgc gtgcgccgac agaatcatca
     1501 ggtagtagcc cgaacgcgac caatcggcgc gagccgaagc gagcaagccg gccgacatcc
     1561 agcggcttgg ccaaagcgga tgctggcaaa aggccagccg gttctgcata ttaaccagcc
     1621 agtcggagct cagcgtgtcg ccaggcgtct gccagagccg gaccgcaaca ccaaccacca
     1681 cggccaccgt cgcgatcacc gccagcgaga gaacaagctt ttgctgtttc gggaatatgt
     1741 tggcgatcac gatcgccgtg gttgcgccca agctccccgc catcaagacg aacatgaaca
     1801 gataaataac gaacatcaca taaaaggctg cgggtgcttt gaccgttaga ccataggccg
     1861 ccatcaatgg gctgcccaga aggaaaaagc cccagctcga gatcgccacc gcctcggcaa
     1921 acttgtgagc gaaaatccgg tccgtcgatg ccggggtgga gagcaggaaa gcaccttcgc
     1981 gcgaatgaaa cagcgcggtg taggtgatga ttcccgtcga gaagaagagc atgatcgaga
     2041 tcgacaggaa aaacatgctg aacagatatt cgaaaatcgc ctgaaacagt tcgtcagaga
     2101 ggccgatgag ctcgaaaccc cccaggaaca agccgagcag tccgacccag aagacgacgc
     2161 tgcaaaacag gatcattgca acgcgcagcc gcgagccggc caggagcgat cggatcgtat
     2221 tgcgcgtcag gcaccagcgc agattgcgta gagcgcgcgc cgtctgtatg gccttgatcg
     2281 ggggtatcgt gtatcgattg ggatcgggga tcgccaggct catggttgca gctcggccag
     2341 ttcagatgag accacgccgt tcgtcgcgag gcgatggtcg ccggtcagtt tcaagaacag
     2401 gtcttccagc ggtccgcgat gctgcacctg cccgcgcagt tgctcgagcg ttcctaatgc
     2461 cagcatccgg ccgtgatcga caatgccgat ccggtcggca agctcttccg cgatggctag
     2521 caggtgagtt gacatcaata cggcgcagcc cgatcgagcc tgtgatatga acagatcctt
     2581 gacgacacgc gcgctccgcg gatcgagccc gactaaaggc tcgtcgacta ccaagacctt
     2641 gggatcgtgt accagcgccg aggcgaaaac gacacgttgt ttcatacctt gcgagtagtt
     2701 ctcgcacagc tcgtcgacaa aatcgcccat ctcgaacgtc tcgatcagct catcgatccg
     2761 ctgggcagcc cgccgcggct cgaggccgta catctcgaca acgaaccgga gaaactcgcg
     2821 gccggtcagt ttgtcataga ggtaaggctg atcgggcacg taacctaaga gctggcgggc
     2881 ctgctgactg gacgcgtcga aaccgccgac tcggacggtg cccgacgtag gtgccagaag
     2941 cccgcagatc atcttgatgg tggtcgtctt ccccgcgccg ttgggcccga gaaaagcgaa
     3001 cagttcgccg gggcgaacct ccaggtcgag accgtccacc gcgcacttgg atccaaagcg
     3061 cttggtcacg ccgctgagtg cgatcatggg cctggtaccg tggtcgggag tttggatatt
     3121 tcttcgtcca cgtcagctcg gcgttccatc attagccgaa cgaaaggggt aggcacttct
     3181 tgccgaagaa tgacgccgtc gaagttaacc caggtgcgca aggatattcc acttagactt
     3241 gccttttgca caacctcgaa agcccgcacc gcatcgccgt tccaatggat cagatccttg
     3301 cgcgccacct cgacgcggac gtcttcgacc cgcgccgtga acggattgat tacctgggac
     3361 ttccatttct ggccaacatg tagccccggc aaccgatcca gcgggcccag gacgtcttga
     3421 accacgctcc gaggcaaata gtctatcgac attttccggt tgaaaaactc gaccggccca
     3481 cgcgatacga tttccatctt tttttccttg agctcgccct tgacggcgat caaggtctcg
     3541 gtcgaatcgc gcgacttgac gtcgaggtta aacgaattaa ggttgcccgt agagtccact
     3601 ctatagacgc tctcgactct gagcctgaag ctcggcgacc gacccaggtg cgggtttcct
     3661 ttcaagagt
//
LOCUS       DATAQL010001281         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1048238, whole genome shotgun sequence.
ACCESSION   DATAQL010001281 DATAQL010000000
VERSION     DATAQL010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048238"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            500..>1888
                     /locus_tag="VHS97_22050"
     CDS             500..>1888
                     /locus_tag="VHS97_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450954.1"
                     /translation="MSGQVQAGGGAVARSAVTLWAASAGAPTELAQIQTGPDGRFAID
                     SDASVGDEAILYLIAAGGEPAVNKTGGANNAIAFLALLGSKPPATVVVNEFTTVASVW
                     TATQFLDGTVIKGHPLGLRIAAGNVPNFVDLTTGGWGDAIQGPLNSGQTPTMANFATL
                     ADLLSGCSTRVKADACDKLFIAATPPKGGTPTDTLTAAQSVASYPWYRPERLFALLGE
                     FYPVPPGKNMRAVPFMPYLSFKPSAWVLPLKFDGGGYRAGGKAMFDSEGNLWVADNWT
                     VGWQGQDSLWQGNATKFAPNGRPLSPMTTGFAGGGMQGGPFGTAVDAKDNAWFTNYGS
                     KAIAVFDKNGKPLTAPDGINFDGRLGLMQGIIVTPSGDVWALGIEKSQLVHFPNGDLT
                     KGRIVCEGDKQEPCKSFRAPFHLGIDQQDRIWVSNSGVDHVTRFPAADPSKAENFKTG
                     INNSGLGIDSQGN"
BASE COUNT          367 a          585 c          597 g          339 t
ORIGIN      
        1 cgacgactgc agcgagcggc cgagatgtgg tggatcgccg accttcgcca acctcgcggg
       61 gaacggctag gaggcgccga gagcagtcat tccggtgaga gcgatttgac tccgggttcg
      121 acccactccg gccattcgcc ggacgtaggg ccatcggtca ctcgtgaacc aaagtggcca
      181 ttccgaggcg cgcattggac ctgttaagtt ggccggtcga cccgtgaccg ttgccatttg
      241 aaccttttgg ctacaaacgg ttgcagctca aggtcattta cgaacgaatt ggcccgtctc
      301 gcaccaaagc acgatatcgc ctggatcgat catgtggcag taatgctcag gacgaatgac
      361 gcatgatcaa gaatttctgt cgggaatgtc ggttaaatcc tctacgaggg agagacagat
      421 gttgaaaata agcagagcgc tcgcaatcgc tacggtgttg atctcggggg ccgccagccc
      481 ggccctggcc gctgttcact tgagcgggca ggttcaggcg ggcggcggcg cggtcgcgcg
      541 ctcagcagtc acgctctggg cagcaagtgc cggcgcgccg acggagctgg cacagataca
      601 gaccggtcct gacggccgat ttgcgatcga cagcgatgca tcggtcggcg acgaagcgat
      661 actgtatctc atcgcggcag gcggcgagcc cgcggtcaac aaaactggcg gggccaacaa
      721 cgcgattgcc ttcctggctc tgctgggcag caagccaccg gccacagtcg tcgtcaacga
      781 gttcaccacc gttgcctctg tctggacggc gacgcagttc cttgacggca cggtgatcaa
      841 gggacatccg ctcggcctgc gcattgccgc cggcaatgtt ccgaacttcg tcgatctcac
      901 gacgggcggt tggggggacg ccattcaggg cccgctcaac agcggccaga cgcctaccat
      961 ggccaacttc gccacgctgg ccgatttgct ctccggttgt tccacgcggg tgaaggccga
     1021 tgcatgcgac aagcttttca tagcggccac gccgccgaag ggaggcaccc cgaccgacac
     1081 tctgacggcg gcccagtccg tcgctagtta tccgtggtac cggcccgaga ggctgtttgc
     1141 gctgctgggc gagttctatc cggtcccgcc aggcaagaac atgcgcgcgg ttccgttcat
     1201 gccgtatttg agcttcaagc ccagcgcctg ggttctgccg ctcaagttcg acggtggcgg
     1261 gtatcgtgcg ggcggcaagg cgatgttcga cagcgagggc aacctctggg tcgccgacaa
     1321 ttggacggtc ggctggcagg gccaggattc gctctggcag ggcaacgcca ccaagttcgc
     1381 gccgaacggc cgcccgctct cgccgatgac caccggcttc gccggcggag gcatgcaagg
     1441 cggccccttc ggtaccgctg tcgacgccaa ggacaatgcc tggttcacca actatggcag
     1501 caaggccatc gcggtattcg acaagaatgg caaaccgctc acggccccgg atggcattaa
     1561 cttcgatggc cggctcggtc tcatgcaagg catcatcgtc acgcctagcg gagacgtctg
     1621 ggctctcggc atcgaaaaga gccagctcgt gcacttcccc aacggtgatt tgaccaaggg
     1681 ccggatcgtt tgcgagggcg acaagcagga gccgtgcaag tcgttccggg cgccatttca
     1741 cctcggaatc gaccagcagg accggatttg ggtgtccaat agcggcgtcg accacgtcac
     1801 gcgcttcccc gccgctgatc cgagcaaggc cgagaacttc aagaccggga ttaacaatag
     1861 cggtcttggc atcgacagcc agggcaac
//
LOCUS       DATAQL010001282         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4403961, whole genome shotgun sequence.
ACCESSION   DATAQL010001282 DATAQL010000000
VERSION     DATAQL010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4403961"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VHS97_22055"
     CDS             <1..445
                     /locus_tag="VHS97_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450955.1"
                     /translation="QVTDLASRKRLSQSAIIEAAVLSFLSPDGADRREAAFTRRLDRL
                     ILQIQRLERNTGVTMETLAVFVRFLLTITPTLPPDAQAAAQAKGQQRYEGFVEALGQR
                     LQKGQSFLNEIPEDVEKKQASARDDGLGRGQGDGSKTRSKMQNTV"
     gene            736..1686
                     /locus_tag="VHS97_22060"
     CDS             736..1686
                     /locus_tag="VHS97_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX3450956.1"
                     /translation="MEAAIWGLLGQRPVDVIDFDGFGHFWAVDVVEQLQVHGSRILKA
                     PPGEVPDLKITNWRHDVVLVANGTSAGTRIPDDDWIPANREGLVICDASSAAFAMHLP
                     WHKLDAVTWSWQKALGGEGAHGMLALSPRAVERLNTHQPAWPIPKLLRLVEDGRFRED
                     VFRGGSINTPSQLCVEDALDALRWAKTVGGLPALLERTDANYAILARWVEQTPWIAFL
                     AKEERIRSHASVCLSVVAPWYERLPLDGKAASIQRMTALLAAERVAYDIDANPMAPPG
                     LRIWCGPTVERDDIQALLPWLDWAYEIVSVEYELRSLASA"
BASE COUNT          334 a          507 c          532 g          326 t
ORIGIN      
        1 gcaggtcacc gatctcgcca gccgcaaaag gctgtcgcag tcggcgatca tcgaggcggc
       61 ggtcctgtcg ttcctgtcac cggacggtgc cgatcgccgc gaagcggcct tcacgcgccg
      121 tctcgaccgg ctgatactgc agatccagcg gctcgaacgc aacaccggcg tcacgatgga
      181 gacgttggcg gtgttcgtgc gtttcctgct caccatcaca ccgacgttgc cgcccgacgc
      241 ccaggcggcg gcgcaggcga aaggccagca gcgctacgag gggttcgtcg aagcgctggg
      301 gcagcggctt cagaaggggc agagctttct gaacgagatc ccggaggacg tcgagaagaa
      361 gcaggccagc gctcgcgatg acgggttggg gagaggccaa ggagacggca gcaagacacg
      421 ctcgaaaatg cagaacaccg tttgaaccag tatcccgatc ttaccgcata tctggaggca
      481 tcaaatgatg gacgacagaa ttcatcttcc cgggcgacct gcctgcaagc cacgtaacgc
      541 acaattcatg tcgggtccct gtccgaagag acctggatac gatgtcacga ccttggatac
      601 gagcttgctc ggccgttcac ggcgagccga agaaacgacc gcccgccgcc gggaagtgat
      661 cgatctgtca aaacagatac tcggcgtccc cgacacctgg catcttggga tcattcctgg
      721 atctgacatg gggccatgga agccgccatc tgggggttgc tggggcagcg gccggtcgat
      781 gttatcgatt ttgatggctt cggtcatttt tgggcggtcg atgtcgttga gcagttgcag
      841 gtccacggct caaggatcct caaggcgccg cccggcgagg tccccgatct caaaattacc
      901 aattggcggc atgacgttgt tctcgtcgcg aacggcacat cagcgggaac gagaatccct
      961 gatgacgact ggattcctgc caaccgcgaa gggttggtca tctgtgatgc atcttccgcc
     1021 gctttcgcga tgcatctccc ctggcacaaa ctcgacgcgg tcacctggtc ctggcagaag
     1081 gctttgggag gggagggcgc tcacggcatg ctggcgcttt cacctcgcgc cgtcgagcgg
     1141 ctgaacacgc accagccggc atggccgatc cctaaattat tacgcctggt ggaggatggc
     1201 agatttcgcg aagatgtgtt tcgcggcggg tcgatcaata cgccgtctca attatgcgtc
     1261 gaagacgcac tcgacgcttt gcggtgggca aaaacagttg gcgggctacc ggccttgctc
     1321 gaacgaaccg acgccaatta cgcgatattg gcccgatggg tcgagcaaac gccctggatc
     1381 gcgtttcttg caaaggaaga acgcatccgt tcccatgctt cggtctgtct gtccgtggtc
     1441 gcgccgtggt acgagcggct gccgctcgac ggcaaggccg cctcgatcca gcggatgaca
     1501 gctttgctgg cggcggagcg cgtcgcctac gacattgacg ccaatccaat ggcgccgcca
     1561 ggattgcgga tctggtgcgg cccgacggta gagcgcgacg acatccaggc gctcctgccc
     1621 tggctcgact gggcctacga aatcgtatca gttgaatatg aattgcgctc tctcgcttcg
     1681 gcttgaatgc cgcggctct
//
LOCUS       DATAQL010001283         6774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5474286, whole genome shotgun sequence.
ACCESSION   DATAQL010001283 DATAQL010000000
VERSION     DATAQL010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5474286"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHS97_22065"
     CDS             <1..202
                     /locus_tag="VHS97_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450957.1"
                     /translation="TGSLIASICMHGAFNGLSTLMMFVALLVGPEVDRKKILPEAMHA
                     LASPIDDGKQRVIHAPKLIQSL"
     gene            681..878
                     /locus_tag="VHS97_22070"
     CDS             681..878
                     /locus_tag="VHS97_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450958.1"
                     /translation="MNEPKSKKPAPLHEGFEWAGAVARVRRGGRTGRPGRRTALLSML
                     TAAYANSSRGTRTTDRTGAER"
     gene            1053..1580
                     /locus_tag="VHS97_22075"
     CDS             1053..1580
                     /locus_tag="VHS97_22075"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450959.1"
                     /translation="MPTPIARPKPPATFEYHYRDHPLVVSRGSPLPVGATATPSGINF
                     VLICRHGTDVWLVLSETCSSVIHAEIPLDPLTNRTGDHWHVRVDGLPEEFCYGYRVDG
                     PQGNGQRYDPENILLDPHARALSCGRPWSTGGTLPRRSLMTEFMRPSARDESANSLGR
                     HDHLRAAYTRLYDRP"
     gene            1507..3201
                     /locus_tag="VHS97_22080"
     CDS             1507..3201
                     /locus_tag="VHS97_22080"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3450960.1"
                     /translation="MNPRIPLEDTIIYELHIRGYTIDPNSRVKYPGTYAGLVEKIDEL
                     KALGITAVELLPVDEFDENDCAFVNPLTGEKLRNFWGYSPILFCAPKAAYAHNLERSG
                     PWQEFREMVDAFHETGIEVYLDVVFNHTAEGGEKGPTYNFRGLDNSLYYMLDEQGRYL
                     NYSGCGNTFSSDHPVVRNFLLDCLRTWVTEGGIDAFRFDLASVLGRDRYGNVLVEAPV
                     VKRISEDSLLRDTKLIAEPWDAAGLYQVGTFPGEGRWSDWNGRYRDDVRRFWRGDPGL
                     TSALATRLCGSDDLYQGRGPLHSINYICCHDGFTLHDLVSYNHKHNEANGEGNRDGSD
                     ANWSWNCGVEGPTLDPAVLAIRNRQVRNLMATLMISQGVPMIMGGDEFLRTQNGNNNA
                     WCQDNPLGWVDWSLKDRNADFVRFVRLMIAFRKAHHVLRRRTFFTGDRSGLPPEIVWH
                     GVDPAKPDFSYNSRSLAWVLDGRRSDRPQQIDRDIYVAMNAYWEPLPFRVPAAPSGRS
                     WRRAVDTALLPPEDIIEEDKRPHVPVLQVYRVQPHSMIILVSEAGDIRRWEHDGRA"
     gene            complement(3338..3410)
                     /locus_tag="VHS97_22085"
     tRNA            complement(3338..3410)
                     /locus_tag="VHS97_22085"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3375..3377),aa:Asn,seq:gtt)
     gene            complement(3582..3743)
                     /locus_tag="VHS97_22090"
     CDS             complement(3582..3743)
                     /locus_tag="VHS97_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEX3450961.1"
                     /translation="MPETVVHFQIRMPPLLHEQLASWAKEDKASLNALIVGILEKAIE
                     VHEKSGHKE"
     gene            4150..4713
                     /locus_tag="VHS97_22095"
     CDS             4150..4713
                     /locus_tag="VHS97_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX3450962.1"
                     /translation="MENSPGGVNHNTSLPLVDSERLIKEFVGHWGLMARAWGINSTMG
                     ELFALLYITGTDWTADDLRHWLGVSRGNVSMNLRELLLWGVVHKVHRAGERRELFRAE
                     TDVWTLFRKILSERKRRELDPSLSVLDRAFQAALEDPSLQELKGRIEPLRRFFRLIES
                     VAVQLLSVPAADLAEFSRIVRDDDPNP"
     gene            4968..6116
                     /locus_tag="VHS97_22100"
     CDS             4968..6116
                     /locus_tag="VHS97_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX3450963.1"
                     /translation="MSGASTEVYHPGLEGVIASETAIANIEGRDGAGGLEYRGYRIED
                     LAGHVSYEETAFLLLHGDLPNRAQLKEFDARLRASRSIPDALVELFRRIPHDIHPMDV
                     LRSSVSVLAHFDPDSSAAPTDHAANVRKAERLVAQMATAVAVRERIASGLPVIPPRDD
                     LDHAANFLNMVNGKVPSKTMRDAFDISMVLYTEHELNASTFAARVTVSTLSDIHSGIV
                     AAVGTLKGPLHGGANEEAWKVLERVGSPENAERWIQDALARKERIMGFGHRVYKTGDP
                     RAAILKKYCADLAREVGDDRWERIAEPIEQAVTAQKLLPPNVDWPSARLYHYMGLDID
                     LYTPIFAMARVAGWAAHIIEQLDHNRLMRPRALYIGPPHRDVKPIVDR"
     gene            complement(6303..>6774)
                     /locus_tag="VHS97_22105"
     CDS             complement(6303..>6774)
                     /locus_tag="VHS97_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450964.1"
                     /translation="AGGTIDFDLPPADLGKETGTLSPRIDWYVIDNRMPLREVFAHKN
                     ATVEVSQEKLNQRVGEVVREARLNGIRPMTIEHLLAFLGYDMNATLTGRPEGVDANAL
                     KRLTLPRRPAESTKPSVGTTTKADTNGDEMPAEKPQAKTKKAPTTKKADNGNEP"
BASE COUNT         1350 a         1902 c         2053 g         1469 t
ORIGIN      
        1 caccggcagc ttaatagcat caatttgtat gcatggcgcg ttcaacgggt tgagtacgct
       61 catgatgttt gtcgcactcc tggtcggacc tgaggttgac aggaaaaaga ttttacccga
      121 agccatgcac gcattggcgt ctcccatcga cgatggcaag cagcgtgtca ttcatgcgcc
      181 taaattgatt cagtcgttgt aatgcaacgt gagcgattaa aaaaacgaaa atcatgcgat
      241 tttttctaga cgccggcgca ccagaacgtt attgttctgg tggtgtcgtg ccaagtctca
      301 ttacggctgg cgggtgaatt cgtttcgccg gccagcctcg ttgtgtctgg tattcgggta
      361 cctgaatcgc caatgatgtc gcagatgaaa gttgaatcgc gagttatccg atgggttaaa
      421 gaccaggttt ggcgatttgg ggacgggtcg tggcgattgt tctgatcgct cgattgtgtg
      481 tgccggcgat ggcggggtga tcactgacgc ccgaccatcg cgagtcgatc cgtaaggctt
      541 cttgtgccgt gcggattctc ggatcaccga tgccaggacg gccgtgctgg ggggaatttg
      601 ggttctccct ggtccaatgg aatggtggag cgtggacctg ttattgtggg tcgcgtttct
      661 ccttgtggga gttgtgtctg atgaacgaac cgaaatccaa gaagcccgca cctttgcatg
      721 aaggttttga atgggccggg gctgtcgccc gggtccgtcg tgggggccgt accggtcgtc
      781 ctggccgtcg cacggcattg cttagcatgt tgaccgcggc ttatgccaac tcgagccgtg
      841 gcactcgcac caccgaccga accggggccg agcgctaaat cctgaaatgt gaacctttga
      901 ataggcgagg ctgcctcgct cgaagcgaaa ccaacggaac aaaagagaga cccgagccga
      961 acgtcttcaa cgacgcaaca gcgtccgaag aggttcggct tgtttttttg gtataacgca
     1021 gtggtacagc ttaatgaaac gcgaaggttg acatgccaac accgatcgca aggcctaagc
     1081 cgcctgccac gttcgagtat cactatcgtg accatccgct cgttgtgtcg cgcggcagtc
     1141 ccctgcccgt cggcgcaacg gcgacgccca gcggaatcaa cttcgtactg atctgccggc
     1201 acggaacgga cgtgtggctc gttctttccg aaacctgctc cagcgtgatt cacgccgaaa
     1261 tcccgcttga cccgctcacc aatcgtaccg gcgatcactg gcatgtccgg gtcgacgggc
     1321 tccccgagga gttttgttac ggctatcgcg ttgacggacc ccaaggaaac ggccagcgct
     1381 atgatccgga gaacatcctt ttggacccgc atgcgcgggc gctgtcgtgc ggccgacctt
     1441 ggtcgacggg aggaaccctg ccgcgccgca gcttgatgac ggagttcatg agaccttcag
     1501 cgcgtgatga atccgcgaat tcccttggaa gacacgatca tctacgagct gcatatacgc
     1561 ggctatacga tcgaccctaa ctccagggtc aaatacccgg ggacctatgc cgggcttgtc
     1621 gagaaaatcg atgaactgaa ggctttaggg attaccgcgg ttgagctgct cccggtggat
     1681 gagtttgacg agaacgactg cgctttcgtc aaccccttga cgggtgaaaa gctgcggaat
     1741 ttctggggct atagcccgat tctattctgc gcgcccaagg cagcgtacgc gcataacctt
     1801 gaacggtcgg gcccgtggca ggaatttcgc gagatggtcg acgcatttca cgaaaccggc
     1861 atcgaggttt acttggacgt cgtcttcaat cacaccgccg agggggggga aaaggggccc
     1921 acgtataact tccgcggcct ggacaattcg ctctattaca tgctcgacga gcagggccga
     1981 tacttgaact acagcggatg cggcaacact ttcagcagcg accacccagt tgtgcgtaac
     2041 ttcttgcttg actgcttgcg cacctgggtc accgagggag gaatcgacgc tttccgtttc
     2101 gatctggcat cggtgctggg ccgggatcgg tacggcaacg tgctcgtcga agcgcctgtc
     2161 gtcaagcgga tctcggaaga ttcactcctc cgcgatacca agctgatcgc cgaaccctgg
     2221 gacgcggcgg ggctttacca ggtcgggacg tttcccggcg aaggccgttg gtcggactgg
     2281 aacggccgct atcgagacga cgtccgcagg ttctggcgcg gcgaccccgg tttgacctcg
     2341 gcgctggcca cgcgattgtg cggcagcgac gatctctatc aagggcgagg gcctttgcac
     2401 tcgatcaact acatctgctg ccacgatggt tttaccctcc acgacctggt ctcatataat
     2461 cacaagcata acgaggccaa cggcgaaggt aaccgcgacg gcagcgacgc caactggagc
     2521 tggaattgcg gcgtcgaggg gcccacactt gatccggccg tgctggcgat ccgcaatcgg
     2581 caggttcgca acctgatggc gaccctcatg atttcgcagg gtgtgccgat gatcatggga
     2641 ggagacgagt ttctccgcac ccagaatggt aacaacaacg cgtggtgcca ggataacccc
     2701 ctgggttggg tcgactggtc cctgaaggat cggaatgccg atttcgtgcg gtttgttcgt
     2761 ctgatgatcg cgttccggaa agcgcatcat gtgctgcgtc ggcgcacgtt ttttacaggc
     2821 gatcgcagtg gactgccgcc ggagattgtc tggcacggtg tcgacccggc caagccggac
     2881 ttttcttaca acagtcgttc actggcatgg gtcctggatg gtcggcggtc cgaccgtcct
     2941 caacaaatcg atcgcgacat ttacgtcgcg atgaatgcat attgggaacc gcttccattc
     3001 cgggtcccgg cggcgccgtc gggccgttca tggcgccgtg cggtcgatac ggcacttctt
     3061 ccaccggaag acatcattga ggaagacaag cgccctcacg ttccggtgct tcaggtgtat
     3121 cgtgtacagc cccactccat gataatcctt gtatcggaag cgggcgacat cagacgctgg
     3181 gagcacgacg gccgcgcgtg acatccgatc gcctggccga ttcttgcggt gaaatctgtg
     3241 tcatctaaag tggtgccgtc tgccgcgatg ggtcgtagcg accagaaagt ttgctccgat
     3301 cgcggtcgcc gagatgccgt cgcgcgaaaa tgataagctc cccgacaagg actcgaacct
     3361 tgaacctagc ggttaacagc cgctcgctct accgattgag ctatcgggga atcatttgtc
     3421 gagcataccg gcacgcatgg tctggctcaa gggcagtcga atcgtggcga gccaggcatc
     3481 aagggagtgt gggagggccg ttgacaccgg ccgagcctgg gcgaaccatt tgggtaaggc
     3541 ccgactcaag ctcggccgga accgaccgaa aaaacgctga cttactcctt gtgccctgac
     3601 ttctcgtgca cctcgatcgc tttttcgagg atgcccacga ttaaggcgtt cagcgatgct
     3661 ttgtcttcct tggcccagct cgccaactgc tcgtgcagta agggaggcat gcgaatctga
     3721 aaatggacca cggtttcggg catagaaaca tctcctcgct acagaaaggg acccgtacaa
     3781 ggattgagtc tgtcgggtac cagccccgcc ttctgccata gagactgaaa cagggcatgc
     3841 aatcttattg tacccgatgt ctcgcgtgaa ctttcattat tttctgtgaa atgatacggt
     3901 ttggtagagg attgtttgta tgaaattgtt ggctgggttc gagatgggga agataagcct
     3961 gatgttgcac ccggatgacg cccctcatca gctcgaatct cgcaatcgcc atgtaggcaa
     4021 gcatccgcgg gtcgaatcac gatcacttgc atgtttgagc tggccctaaa tccgcacaag
     4081 aaccaccggc gtgatgctcc cgaccgaatg acggcacgct ttttctaatt gcctgtctcg
     4141 agtaaattta tggagaattc acccggcggc gtcaaccaca acacgtctct accgttggtc
     4201 gattccgaac gcctgatcaa ggaattcgtg ggtcactggg ggctcatggc tcgggcctgg
     4261 ggaatcaatt ccacgatggg cgagttgttt gcattgcttt atatcacggg gaccgattgg
     4321 acggcggatg atctgcgcca ttggctaggc gtatcgcgcg gcaatgtcag catgaacttg
     4381 cgcgagctgc tcttgtgggg ggtggttcat aaggttcatc gggcggggga gcgtcgagag
     4441 ttgttccgag cggagaccga cgtctggacg ctttttcgca agattttgag cgagcgcaaa
     4501 cgaagggaac ttgatcccag cctgagcgtt ctggatcgcg cgtttcaggc cgcccttgaa
     4561 gacccgtcct tgcaggagct gaaaggccgg atcgagccgc tgcgccgctt ttttcggtta
     4621 atcgaatcgg tggccgtgca acttttgtcg gtgccggcgg ccgatctcgc cgaattcagc
     4681 cgtattgttc gcgacgatga ccccaatccg tgaggtcgtt ccgcgagaac aggcgcatgc
     4741 tgcacgcgtc aggccgtagc aggggcggaa tcggcgcagg gtcatggaac accccggtcg
     4801 attcgtctag actaaaacaa taagatactg ggttttgtgc gatcccttga ctccggcaac
     4861 cggcaccgcg aatagcaccg gccgcgtctg cccgggcggt caatttcacg ctcgtatcta
     4921 cccgttagag tcaatttccg gtggtcctca aagtgcggag ttcggatatg agtggtgcgt
     4981 cgacggaagt gtatcatccc ggcctggaag gcgtgattgc gagcgaaacg gcgatcgcca
     5041 acatcgaagg aagagacggc gcgggggggc tggaatatcg gggttaccgg attgaggatc
     5101 tggcagggca cgtatcctac gaggagacgg cattcctgct tttgcacggg gacctgccca
     5161 accgggccca actcaaggaa ttcgatgccc gtttgcgtgc gtcgcgatcg atcccagacg
     5221 cactcgtcga gctctttagg cgtatccccc acgacattca cccgatggac gtgctacgca
     5281 gctcggtgag cgtactcgcg catttcgacc cggactcgag tgccgcgccg accgaccacg
     5341 ccgccaacgt gcgcaaagct gagcggctgg tcgcgcagat ggcgacggcc gttgcggttc
     5401 gcgaacggat tgccagcggt ctgcccgtca tcccgccccg tgacgatctc gatcacgcgg
     5461 ccaatttctt gaatatggtc aacggcaagg tcccgtcgaa gacgatgcgc gacgcgtttg
     5521 acatctcgat ggtcttgtac accgaacacg agctgaacgc gtcgactttc gccgcccgcg
     5581 tgaccgtttc gacactttcc gacatccact cggggattgt cgcggccgtc ggaactttga
     5641 agggtccgct ccacggtggt gccaacgaag aggcctggaa agtgcttgaa cgggtcggca
     5701 gccccgaaaa tgctgagcgc tggattcagg acgccctggc ccgtaaggag cggatcatgg
     5761 ggttcggcca tcgtgtgtac aaaacgggcg atccccgcgc cgcaattctc aagaaatact
     5821 gcgccgatct agcgcgggaa gtcggcgatg accgctggga gcggatcgcc gagccgatcg
     5881 agcaggccgt cactgcccag aaactccttc cccccaacgt agactggccc agcgcgcggc
     5941 tataccacta catgggtctg gatatcgacc tttacacccc gatctttgcc atggcccggg
     6001 tcgctgggtg ggccgctcac atcatcgagc aacttgatca taatcggttg atgcgaccgc
     6061 gagcgctgta tatcggcccg ccacatcgag acgtcaagcc gatcgtagat cgataggttt
     6121 gaccaaatgg cggtcccgag cggggttaca gagttcggcg ggccgttcag cgggcggcgc
     6181 gcggactcga actgccaaaa cgctaatcca gattgcaaaa ccaagctcgg ttctagccgg
     6241 gttttgcaat ctggactttt gaatgctgag ccaagcagcc gaatcgtcaa accaccgttt
     6301 tactacggct cgttgccgtt atcggccttc tttgtggttg gcgctttctt ggtcttggcc
     6361 tggggctttt cagcgggcat ttcgtccccg ttggtgtcgg ccttcgtggt cgtgccgacg
     6421 ctgggcttgg tcgactctgc tggccgccgg ggaagtgtaa gccgcttgag cgcgttcgcg
     6481 tcgacgccct cggggcggcc ggtgagcgtg gcattcatgt catagccgag gaaggcgagc
     6541 aggtgctcta tggtcatggg gcgaatgcca ttcaatcgag cttccctcac cacctcgccc
     6601 acgcgttgat tgagtttctc ttgtgagacc tcgactgtgg catttttatg ggcgaagacc
     6661 tctcgaagcg gcatgcggtt atcgatgaca taccaatcga tgcgcggcga aagcgtgccg
     6721 gtttccttac cgagatccgc ggggggaagg tcgaagtcga tcgtgccgcc ggcc
//
LOCUS       DATAQL010001284         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1655637, whole genome shotgun sequence.
ACCESSION   DATAQL010001284 DATAQL010000000
VERSION     DATAQL010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655637"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHS97_22110"
     CDS             <1..189
                     /locus_tag="VHS97_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450965.1"
                     /translation="NYAGNGGIGFTETGRVPNGAFGAAIRDFADGLSNTAAVSISLQT
                     WRALGTRNGGEAVSTGPP"
     gene            924..2408
                     /gene="ffh"
                     /locus_tag="VHS97_22115"
     CDS             924..2408
                     /gene="ffh"
                     /locus_tag="VHS97_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX3450966.1"
                     /translation="MFDDLQKRLSSAFRRFRVSGVLTEANMKEGLREVRSALLEADVH
                     YDVVQEFMAKINDEAAGTQLVKSIRPEQQIVKIVHDELIDLMGPVDPSIRFEKAGATV
                     LMLCGLQGAGKTTTCGKLARLLQGQGKKPMLVAADLQRPAAVEQLKVIGSQLGVPVYS
                     KLNSSPVAVCQEALAEANRAGCDTLILDTAGRLHVDDDLMAELTQIEKKVKPHQVFFV
                     CDAMTGQDAVASADAFNKALELDGVILTKLDGDARGGAALSVRKVTGVPIKFVGKGEK
                     LDKLEPFDPERVVGQMLGMGDIVGLVEAAQSAVDEEEARVQQERLAKGKFDLDDFRKQ
                     IVNMKKMGSMRDLMSKIPGLNQMGLDELGGIDADEEIKRIQGIIDSMTPDERSDPTLI
                     DISRRRRIAAGSGVDPADVSGLVKQFDAMAAVVRQMAQMSMLDKIRTLTGLGRAAATN
                     PAARIVPTKVGTGKRLSPKEREKLRKQREKEERRRRREQRNGSA"
     gene            2524..2856
                     /gene="rpsP"
                     /locus_tag="VHS97_22120"
     CDS             2524..2856
                     /gene="rpsP"
                     /locus_tag="VHS97_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX3450967.1"
                     /translation="MVRLRMKSLGRRHRPFFRICAMDSRTGRDGCPIEELGHYDPMCS
                     NSETQTVLNVSRVRYWLSVGAQPSHKVQVLLNKKGVKKPAPGEPWQVAETAAAPATAT
                     EALEPAAT"
     gene            2879..3541
                     /gene="trmD"
                     /locus_tag="VHS97_22125"
     CDS             2879..3541
                     /gene="trmD"
                     /locus_tag="VHS97_22125"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX3450968.1"
                     /translation="MSATPPYRIDVLTLFPPLFDGFLSQSILSRAIDKGLVQIERWDI
                     RSWAQGKHKQVDDRPFGGGPGMVLMPEPVVDATEAVRAAAEPPGHLIMLSPQGRRLDQ
                     RRAAELVEVPRLILLCGRYEGFDERIIEILEPELLSVGDYVLSGGEVAAMVLIDAVVR
                     LIPGVLGDERSAIDESFGPDGGLEYPQYTRPRVYRGRAVPEVLLSGDHSAIALWRLEN
                     RR"
     gene            3737..4060
                     /gene="rplS"
                     /locus_tag="VHS97_22130"
                     /pseudo
     CDS             3737..4060
                     /gene="rplS"
                     /locus_tag="VHS97_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
BASE COUNT          897 a         1234 c         1355 g          832 t
ORIGIN      
        1 aattacgccg gcaacggtgg tatcgggttc acagaaaccg ggcgcgtgcc aaacggggcg
       61 ttcggcgccg cgatccgcga tttcgccgac gggctgagca acacggccgc agtctcgatc
      121 tctctccaga cctggcgagc cctgggcacc cggaatgggg gagaagccgt ctcgacggga
      181 ccgccctagt gggccgtgaa tctcgaatga gcttcgtgat ccagatcacc gtgtttcttg
      241 acatccttat ctcttgtatt cccagatccg acgtgatatg tttggcttag tgggcgaacc
      301 agcctagcag gacgcctaga aaccgtagcc cttcggggtg gagaactgaa gggaagtccg
      361 ccaggttggc caatggtatg ccgaatgtct cgcccactcg gtctgaaggg tagtcggact
      421 ttccaaagag ccgaatgcaa cgccgacaat ttcgggctgg atgaagagat gctccaggcg
      481 gcatagtgaa acttgagcgc cttggagaaa actcccagac ctcgaaaacc ggtaaccgag
      541 gctcccacgt ctccaggcgt catggtttcc ctctagcgca acatcggctg gagacggatc
      601 tatgcgccgc cgaagcccgc aagaccgagc gtcatcggtt gggatgcgtc gaacgataag
      661 cccaacggtc ttgacccggc atacccccca aaaacggctt cccgttttcc ccctaggtca
      721 tccttggggg tttctttttt tgcgcctcgg ttgcgggacc gacggtcgtg actgattgct
      781 tgtcacaatc gagcgatttc gcggaacggc gtgtccccag cccggtccgg tccgatgctt
      841 caaagcacgt tgctaccgaa cgtaacccgc attataatca ttggttcttc ttcagaaatt
      901 ggtcggcggg gttttgacgg cagatgttcg acgacttgca aaaacgcctc tcgtccgcct
      961 ttcgtcgctt tcgcgtcagc ggtgttctga ccgaagcgaa tatgaaggaa ggtctgcgcg
     1021 aggttcgctc cgcactcctg gaggctgatg ttcactacga cgtcgtccag gagttcatgg
     1081 cgaagatcaa cgacgaggct gccggcactc agttggtcaa gagcattcgg cccgagcaac
     1141 agatcgtcaa gatcgttcac gatgagctga ttgacctgat ggggccggtg gatccgtcga
     1201 ttcgcttcga gaaggccggc gcgaccgtcc tcatgctgtg cggtctgcaa ggagcgggca
     1261 aaacgacgac gtgcggcaag ctggctcgat tgcttcaggg ccaggggaag aagccgatgc
     1321 tggtcgccgc cgatctccag cgaccggcgg ccgtcgagca actcaaggtc atcggcagtc
     1381 agctcggagt gccggtttac agcaaattaa actcaagccc ggttgcggtt tgccaggaag
     1441 cacttgctga ggccaaccgc gccggctgtg atacgctcat tctcgacacc gccggccggt
     1501 tgcacgtcga cgacgatctg atggccgagc tgacccagat cgaaaagaag gtcaaaccgc
     1561 accaggtctt ctttgtgtgc gacgcgatga ctgggcaaga tgctgttgct tcggcagacg
     1621 ccttcaacaa ggcactcgag ctcgacggag tcatcctcac gaagctcgac ggcgacgcgc
     1681 ggggcggggc ggcgctctcg gtccgcaagg tcaccggtgt ccccatcaag ttcgtgggca
     1741 aaggagaaaa actcgataaa ctcgagccct tcgatcccga gcgggtggtc gggcagatgc
     1801 tgggcatggg cgacatcgtc ggcctggtcg aggcggccca gtccgcggtc gacgaagaag
     1861 aggcgcgcgt ccagcaggaa cggctcgcca agggcaagtt cgacctcgac gactttcgca
     1921 agcagatcgt caacatgaaa aagatgggtt caatgcggga tctgatgagc aagatccccg
     1981 gattgaacca gatggggctc gatgaactgg gcggaatcga cgccgacgag gagatcaagc
     2041 gcattcaggg gatcatcgat agtatgaccc cggatgaacg aagcgaccca accctgattg
     2101 acatttcccg gcgccggcgg atcgccgcgg gaagtggcgt ggaccccgcc gacgtctcgg
     2161 gtctggtcaa gcaattcgat gcgatggcag cggttgtcag gcagatggcg cagatgagca
     2221 tgctcgacaa gatccggaca ttgaccgggc tgggtcgggc ggccgccacg aatccggcag
     2281 cccgcatcgt cccgaccaag gttggaaccg gcaagcggct ctctcccaag gaacgggaga
     2341 aactgagaaa gcagcgtgag aaggaagaac gccgccggcg ccgtgaacaa cgaaacggat
     2401 ccgcgtagtt cgggtccatg ccgacgcgtc gggtgacgcc ccaccaggag ttcgccgtgc
     2461 cggcaaattt ctgtgtattt gacttttgtt ttctgtgtaa ttgactttta gaggagtatt
     2521 ggtgtggttc gacttcggat gaaatccctg ggacgcaggc accgaccctt cttccgcatc
     2581 tgcgccatgg attcgcggac gggacgagac gggtgcccta ttgaagagct ggggcactac
     2641 gacccgatgt gctcgaattc cgaaacgcag acagtcctga acgtcagccg ggtcaggtac
     2701 tggttgtcgg tgggcgcgca gccgtcgcac aaggttcagg tgctgctcaa caaaaagggt
     2761 gtgaagaaac ccgctccggg cgagccgtgg caggttgccg agactgcggc cgcgccagcc
     2821 acggccacgg aggcgctcga gccggccgct acctgaccgg agacggacgt cgcgttttat
     2881 gtccgcaaca ccgccgtatc gcattgacgt gctgacgctt ttcccgcccc tttttgacgg
     2941 tttcttgagc cagagcattc tgagtcgggc aatcgacaag ggcttggtcc agatcgagcg
     3001 gtgggacatt cgctcctggg cccaggggaa acacaagcag gttgacgatc ggccgtttgg
     3061 cggtgggccg ggcatggtct tgatgcccga gccggtagta gatgcgaccg aggccgtccg
     3121 ggcggcggca gagccgcctg ggcacttgat catgctctca ccgcagggac gccgactgga
     3181 tcagcgtcgc gcggccgagt tggtcgaggt cccgcgatta attttacttt gtggtcgtta
     3241 cgagggtttt gatgagcgta tcatcgaaat actagaacca gagctgctct cggtgggcga
     3301 ctatgtcctc tccggaggag aggtcgccgc gatggtcttg atcgatgcgg tcgtgcggct
     3361 gattcccggc gtgctcggcg atgaacgaag cgccatcgac gagtcgttcg gacctgatgg
     3421 aggactcgaa tatccccaat acactcgtcc tcgcgtctac cgcggccgcg cggtccccga
     3481 agtccttttg agcggcgacc actctgccat cgcactctgg cggcttgaga atcggcggtg
     3541 aaataccctg ttttacgctt tctcggcccg ctagccattc gccgggcgat cccgctctaa
     3601 cacggccgat ggcctcccgg caaccggcgt gcgcttggcc gtataaaact cttgatcttg
     3661 tactaaagtc tataaatggg gccgcgcggg aacgctttcg gcctagagaa aggaagtttc
     3721 gatgcagaac aaattgatgg atgtggtgga aaaaaccacc atgaaagcca cgatcccgtc
     3781 attcgagatt ggcgatacgg tcgatgtgca cgtgcgaatt ctcgaaggcg acaaggaacg
     3841 gatccagatt tacaacggcg tcgtgatcgc tcggtccgga agcggaccgc gtgaaatgtt
     3901 cgtcgtgcgc cgcatagtgc agggggaggg tgtcgagcgc aaattcccgc tgcagtcgcc
     3961 gcggatcgcc gacatcactg tcaagcggtc gggcaaggtc cggcgcgcca agctctacta
     4021 tctgcgtgat cgctcgggca aggccgtcag gctcaaggag aggttcacca gcacggcggc
     4081 tacgagcgag gccaaggcgg cggccaagag cgaacgccgc aaggtcaggg ccgccaagaa
     4141 agagaaagac aaggtaaagg ccgcgacctg agcacgcccg cttcgcagcc tgtttggaag
     4201 caatgtggct ggaagctcgc tgaccatcct caatttggat ttcagcgtgc ctgatagctc
     4261 actgaccacg ctcaatttga aaatcagcgt gtctgatagc tcactgacca ccccccct
//
LOCUS       DATAQL010001285         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1963059, whole genome shotgun sequence.
ACCESSION   DATAQL010001285 DATAQL010000000
VERSION     DATAQL010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963059"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VHS97_22135"
     CDS             complement(<1..612)
                     /locus_tag="VHS97_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450969.1"
                     /translation="MAGSIDVPIAGSPSHLLVPGKPVVLVPYYSVIEKECEEGLRSLV
                     KAGLALSRFSLSAIDLLRSAMLSHALKDGFDQFLFIDADIGFDPRDAIRLFNRPEPVI
                     AGVYVHNKGRDFAGTFARGVTKVVFGPAAPGPYPMLYAPTGFLRIRAEVLRRMIDELT
                     LPECHWGSIKGIYPFFLPLCVPDGRGGTRYLTEDYSFSHRLAQI"
     gene            829..1416
                     /locus_tag="VHS97_22140"
     CDS             829..1416
                     /locus_tag="VHS97_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3450970.1"
                     /translation="MARHIARAAARLFAERGYDATSVREIVEAAGVAKPTLYYYFRSK
                     EGLAQALITVPLSNLTATLQRIVSQVEDPITCLEQVVDAQYNFCREDPDRGRFIYALL
                     FGPLGSEVASELEPFKESLSGWTDAAVRRLAEVQIVPRHRAEACSTACRGLIVISTLD
                     YLYRDKPLGRDLARQNVNDLLRGFGDSRYTKELES"
     gene            1419..2822
                     /locus_tag="VHS97_22145"
     CDS             1419..2822
                     /locus_tag="VHS97_22145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3450971.1"
                     /translation="MSWRSSWSNLETLASAIFLAGVAGCGHGGSEADIERSTAARVVP
                     VTVANLEHRSVERTVEVIGTLRGWEQVTVGTKRTGRVVKVHHDIGDRVKPGAPLVELD
                     PVDARLGVQQAESRYLGELIKLGITRRQAEEIVAKYGISEELLIGQVADDAIAKVPSV
                     IQKRVACDRAQQHLTRQRALTQRGAGTPQELEDAETDWRTAAANYDDAVQTARTVIAN
                     AIASKVSLSQAGQTLLDMTIRAPTPKLIPPSLTRTSVLSYGLTKRQVSEGQMIKEGES
                     IAELVIEDPIRLWSQVPEQYAEDVQLGQRVRLTTRAHPGVAIEGKIARINPAVETASR
                     TFQVETLVPNERGLLRPGGFAKASIIVDAEARAAIVPTESVVRFAGVTKLFIVENGKA
                     RSINDIETGAEGRGWVEVTSKLLPHAAQIVTTGQTQLAEGTPVLVREPEPPTEPKLGE
                     TDTIPETAATANVPSRR"
     gene            2868..>4189
                     /locus_tag="VHS97_22150"
     CDS             2868..>4189
                     /locus_tag="VHS97_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3450972.1"
                     /translation="MSFLPLYPGIPMTLSDVCINRPVFTWVLVAIPVVLGLFSYGELG
                     VDLFPDVDFPVCTVTTVLQGASVEEMETTVTKPIEDIINTVSGIDELRSTTTEGVSIV
                     TVQFLLSKNGDVGMQEVRDKVNTILAELPDGTDPPIVDKFDTSSMPVMTIAVSGRRDF
                     REVTELARKQIKERLETVNGVGSINLVGGRVRAMNVVLDTERLAGYNLSVEDVRIALT
                     RQNLEVPGGRMDQGARELVLRTLGRLRTQKEFNNLIVAHRNGYPIRLSDLGRAEDAFE
                     EPRSQARLDGESAVSLVVQKQSGVNTVKVVEDVKARLDLLKAALPADITTEVIRDQSR
                     FIKKSIEEVKFHLLLAAVLVSATILLFIRDWRTTLIATLAIPTSIIPTFMFMRYMGFT
                     LNNITMLGLILAIGIVIDDAVVVHENIFRHMEEDGMDGMAAARRGTRE"
BASE COUNT          895 a         1286 c         1247 g          761 t
ORIGIN      
        1 gatctgcgcc agccggtgag agaagctgta gtcctcggtg aggtaacgag ttccgccgcg
       61 tccatcgggg acgcacagcg gcaggaagaa cggataaatt cccttgatcg atccccaatg
      121 gcattctgga agcgtgagct catcgatcat tcgccgcaaa acctcggctc ggatccgtaa
      181 gaatccggtc ggtgcgtaga gcatgggata aggcccaggg gccgccgggc cgaaaacgac
      241 tttcgtgact cctcgggcga acgttccggc aaaatcgcgt cccttgttat ggacatagac
      301 ccccgcgatg accggctcgg gcctgttgaa gagccggatt gcatcgcgcg gatcaaagcc
      361 gatgtcggca tcgatgaaaa gaaactgatc gaagccgtcc ttgagcgcgt gagaaagcat
      421 cgcacttctc agcaggtcga tggctgacaa cgagaagcgg ctaagcgcca atcccgcctt
      481 gactaacgag cgcaagccct cctcgcactc tttctcgatg actgagtaat agggcacaag
      541 caccacaggt ttgcccggaa ccaaaaggtg cgaaggcgaa cccgcaatcg gcacgtcgat
      601 cgacccggcc acgatttgcg tcctctctaa atccctgagt gtctcacata cggcgatcca
      661 ttacttttcc gaattcgaca caagcggcac ttgaccaaaa tgcggattgg gtgcacaata
      721 cctaccgctc ggtagaactt gcgggtttta ggccaatacg ggcattttgg gtcgtttgtg
      781 taacacatga cgatcgctcc ggcttccaaa aaagaagagt cgagccctgt ggcgcgtcac
      841 atcgcgcgag ctgccgctcg tctttttgcg gaaagaggat acgacgccac gtctgtacgc
      901 gagatcgttg aagcggcggg ggtcgccaag cccacacttt actactactt tcgaagcaag
      961 gaaggattgg cgcaggcgct catcacagtt cccctgtcca acctgacggc gaccctccag
     1021 cggatcgtct cacaggtcga agatcccatt acctgcctgg aacaggtcgt tgatgcccag
     1081 tacaacttct gccgcgagga cccggaccgg ggccggttca tttatgctct tttgtttggc
     1141 cctctgggat cggaagtagc cagcgagctg gagcctttca aggaaagcct ctcgggctgg
     1201 accgacgcgg cggtgcgccg ccttgcggaa gtgcaaatcg tgccccggca ccgcgcggaa
     1261 gcctgttcga ccgcttgccg cgggctgatc gtcatttcaa cgctcgatta tttgtatcgt
     1321 gacaaacccc tgggacgcga cctggcacga cagaacgtga atgacttgtt gcgcggcttt
     1381 ggcgacagcc gatacactaa agaactggaa tcataattat gagttggcgg agtagctggt
     1441 caaatttgga aacgctggcc tcagcgatct tcctggccgg agtggccgga tgcggtcacg
     1501 gagggtcgga ggccgacata gaacgatcga ccgcggccag agtcgtgccg gtcacggtcg
     1561 cgaatctcga gcatcggtcc gtcgaacgga cggtcgaggt catcggcacg ttgcggggct
     1621 gggagcaggt gaccgtgggg accaagcgga ccggccgggt cgtcaaggtc caccacgaca
     1681 tcggcgaccg ggttaagcct ggcgcgccct tggtcgagct cgacccggtc gatgccaggc
     1741 tgggcgtgca gcaggccgag tcgagatact taggtgagct gatcaagctg ggaatcacca
     1801 gacgccaggc cgaggagatc gtcgcaaaat acggaatcag tgaggaactc ttgatcggcc
     1861 aggtcgccga cgacgccatc gccaaagtgc cctctgtcat ccagaaacgg gttgcttgcg
     1921 atcgagccca gcaacacctg acgcggcagc gtgccctcac ccagcgcgga gccgggacgc
     1981 ctcaggaact cgaggacgct gaaaccgact ggcggacggc cgccgcgaac tacgacgacg
     2041 cggttcagac ggcacgtacc gtcatcgcca atgcgatcgc atccaaggtc agcctcagcc
     2101 aggccggcca gaccttgctg gacatgacga tccgggctcc cacgcccaag ctcatccctc
     2161 ccagcctcac gcgcaccagc gtcctctctt acggactcac caagaggcaa gtgtccgagg
     2221 ggcagatgat caaggaggga gaatcgatcg ccgaactcgt gatcgaagat ccgatccgcc
     2281 tctggagcca ggtgcccgag caatatgccg aggacgtgca gctcgggcaa cgagtacgtc
     2341 ttacgacccg ggcccacccc ggcgtggcca ttgagggcaa gatcgcccgg atcaaccccg
     2401 cagtggagac cgcaagccgt acttttcagg tagaaacatt ggtgcccaac gagcgcggac
     2461 tgctgcggcc aggcggtttt gccaaggctt cgatcatcgt cgacgccgag gccagggcgg
     2521 ccatcgtacc gaccgaatcg gtcgtgcggt ttgcgggtgt caccaagctc ttcatcgtcg
     2581 aaaacggcaa ggcacgctcg atcaacgaca tcgagaccgg agccgaaggg cgcggatggg
     2641 tcgaggtgac cagcaaactt ctcccccacg ccgctcagat cgtcaccacc ggccaaacgc
     2701 agcttgccga aggaacgccc gtcctggtcc gcgagcccga gccccccacc gagcccaagt
     2761 tgggtgaaac cgacacaatt cccgagaccg cagcgaccgc caacgtcccg tccaggcgct
     2821 agcgttcggc caaatccacg tcgccaacgc gtgatcgatt ggattttttg tcctttcttc
     2881 ctctttatcc gggcattccc atgaccctat ccgatgtgtg tatcaatcgg cccgtgttca
     2941 catgggtact cgtggccatc ccggtagtgc tcgggctctt ttcctacggc gagctggggg
     3001 tcgacctctt tcccgacgtc gacttccccg tctgcacggt cacgaccgtg ctgcaaggcg
     3061 ccagcgtcga ggagatggaa acaaccgtca cgaaaccgat cgaagacatc atcaacacgg
     3121 tttcgggcat cgacgaactg cggtcgacca cgaccgaagg cgtttcgatc gtgaccgtcc
     3181 agttcttgct ctccaagaat ggcgacgtgg ggatgcagga ggtgcgcgac aaggtcaaca
     3241 caatccttgc tgagcttccc gatggcaccg acccgccgat cgtcgacaag ttcgacacga
     3301 gctcgatgcc cgtcatgacg atcgccgtct caggccggcg cgactttcgt gaagtgaccg
     3361 agctggctcg caaacagatc aaagagcgat tggaaacggt caatggcgtc gggtcgatca
     3421 acttggtcgg cggccgcgtg cgtgcgatga acgtcgtctt ggacaccgag cgcctggccg
     3481 gctacaacct ctcggtcgaa gacgtccgga tcgcactgac ccggcaaaac ctcgaggtcc
     3541 ccggcgggcg catggatcaa ggcgcccgcg agctcgtgct ccgcacgctg ggacggttgc
     3601 gaacccagaa agagtttaac aacctgatcg tcgcccatcg caacggctac ccgatccgct
     3661 tgagcgacct agggcgggcc gaggatgctt ttgaagagcc acggtcgcag gcgcggttag
     3721 acggcgaaag cgcggtcagc ctggtggtcc agaaacagtc gggcgtcaac acggtcaagg
     3781 tggtcgagga cgtcaaggcc cgactcgatc tcttgaaggc agccctcccc gccgacatca
     3841 ccaccgaagt catccgcgac cagtcgcggt ttatcaaaaa gtcgatcgag gaggtcaaat
     3901 ttcacctcct cttggccgcc gtcctggtgt cggccactat cctcctcttc atccgcgact
     3961 ggcggaccac cttgattgcc actctggcca ttccgacatc gatcatacca acctttatgt
     4021 tcatgcggta tatgggcttt acgctcaaca atatcacgat gctcggcctc atcctggcga
     4081 tcggcatcgt gattgacgat gcggtagtcg tccacgaaaa catcttccgg cacatggaag
     4141 aagacgggat ggacggcatg gcggcggcac gcagggggac gcgtgagat
//
LOCUS       DATAQL010001286         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3643967, whole genome shotgun sequence.
ACCESSION   DATAQL010001286 DATAQL010000000
VERSION     DATAQL010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643967"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHS97_22155"
     CDS             <1..202
                     /locus_tag="VHS97_22155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450973.1"
                     /translation="ASGLPAAASPTRAGEKWAPALDRPARLMMILPVEAAMLSKMDRT
                     RDDDAPGRLKAIKAICGAWSPY"
     gene            291..1253
                     /locus_tag="VHS97_22160"
     CDS             291..1253
                     /locus_tag="VHS97_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450974.1"
                     /translation="MQGTFFSIRTSPARKAVVAKRYLYMTAALAVALLGGGATGAGAQ
                     TLRWKLKSGEVLHYTLDTKQAANFKVMGRDKKSSRSHTINLSWTVKSVSATADAEIAL
                     KFDRIRMRIEQPPFVPLQFDSSPNNLEIPEEFESAERQIKALAGAEFSFELRPNGAVD
                     HLKIPDQTLKNLRDGAGPDAGGQGGISEQGLKDMLLQSSPPSFPAESLEPGKTWANKP
                     SRISAPGLGTLVVDQVYSFQGPDPKDPKMLLIGMEARVALEPAQDVTAKIRAQEGKGN
                     LTFDSESGRIVSSRVSQKMEMVISDRGQDLVQTTETTSTMMLDR"
     gene            complement(1293..4340)
                     /locus_tag="VHS97_22165"
     CDS             complement(1293..4340)
                     /locus_tag="VHS97_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3450975.1"
                     /translation="MLRVLWMGCSLGVLLYSIAVLTHVARMGSIGVRCMFGTKVEEEI
                     AADYIWHDDRPRIGDTLLTIGSSDIHERSYSDYIRALRGLSAQVGETVEVRWRDQHSN
                     EIRSASVKVQYPPSWTYYRSCVWFLQELLIFAIGARVFWKRPDDNSAQLFFAVCIVTV
                     GAFMGGYHWTEIVTEPLLIYPFALFAVFVPVVNLHFFLIFPRANPILLRRRKWVLGAL
                     YGIPTAYLLALWGSMYAARWSALNKDYWQTALAFQMVRRLALGYVVLAAFLFGLCILC
                     LTSSYRNAPSRAERNQVQWILLASLISLVLIAYLLAQAWMDPATLGRGNAAWPMFGVS
                     LLYTMAYAFSITRYKLMQVEEIINRRAVYFAFSLTAGLIYSGVLLVSGKLIGDQSFST
                     HSTWLGAVAAALSVIVVLILSEVARGRVQRVIDRQFFREKYKFDRAMHKMRLAVGSLI
                     DRVTLGRRLLEAAAEVLRLEWGALYLGDAPGRPLQLVASLGPAPEERSLTSDNPLAVR
                     LRQTSAVRLSHALASDGGSDPATDAMIALGGEAATSLGDDGHLSGLLILGPKRSGMPY
                     EDEEMAFLGALSSVATLVLHSADIQQTLESLNQELRDKVEKIAEQKRRILILQDQLRE
                     QAERERDGSGPSPERPLGNPADESNREGDLGPFESIRGVSAAVGKMIAMARKIAVTHS
                     AVLIRGESGTGKELLAAAIHQASPRADGPFVKLHCAALSQSLLESELFGHVKSAFTGA
                     DRDRVGRFEQANGGTLFLDEIGDINLEVQTKLLRVLQEMSFERVGSSQPITVDVRILA
                     ATHQDLEALIRAGRFREDLYYRLNVICLETPPLRERREDVFELAVWFLNLHAQRTGKL
                     VAHIDPEAVEALVAYDWPGNIRELENVLERAVVLADGPAVMFDDLPPELRQPVRRRLR
                     PRLPTMAASRAVSPHGPTSIGTTDVRSRNPVPRDQFPDSIWANSGSEPLPGEDWNSEF
                     VAYERQRLIDALNEAKGNKSVAARLLGMPRSTFFSKVKKHGIV"
BASE COUNT         1071 a         1442 c         1354 g          878 t
ORIGIN      
        1 cgcatccggc ctccccgccg cggcaagtcc gactcgagct ggtgaaaaat gggcgccggc
       61 gctcgatcgg cctgcacgac tcatgatgat tttgccggtc gaagccgcta tgctttccaa
      121 gatggaccgg acgcgagatg acgacgcccc aggcagactg aaggccatca aagcaatctg
      181 cggtgcgtgg tcgccctatt gacagcgcac ttacgtctgc ttcacggaac cgattgcctt
      241 ggaaccgccg gtaaaggatc gagtctttcc cttcgcgaaa ggattgtggg atgcaaggta
      301 cattcttctc gatacgcact tcgccggcaa gaaaagccgt cgtcgccaag cgatacttgt
      361 acatgaccgc ggcgctggcc gtagctttgc tgggaggcgg cgcgaccgga gccggcgcac
      421 aaactctgcg ctggaagctc aaatccggcg aggttcttca ctacacgctc gacaccaagc
      481 aagcggcaaa cttcaaagta atgggccgag ataaaaagtc atcaagatct catacaatta
      541 acctgagctg gacggttaag agcgtttcgg ctactgcgga cgccgagatc gcgctaaaat
      601 tcgaccgcat acggatgcgt atcgagcagc cgccgtttgt gcctctgcag ttcgactcga
      661 gcccaaacaa ccttgaaatc ccggaagagt ttgaatcagc cgagcgtcag atcaaagcgc
      721 tcgccggcgc cgagttttcg ttcgagctcc ggcccaatgg cgcggtggat catctcaaga
      781 ttcccgatca aaccttgaaa aacttgcgcg acggcgcagg accggacgcg ggggggcagg
      841 ggggcatctc cgagcaaggc ctgaaggaca tgctgctcca gtcgagtcct ccttcctttc
      901 cagccgagtc gctcgaacca gggaaaacct gggcgaacaa gccctccagg atttcggctc
      961 cgggcctcgg cactctcgtg gtcgatcagg tctattcgtt tcaaggaccg gatccgaagg
     1021 acccgaaaat gctgctgatc ggcatggagg ccagggtggc gctcgaaccg gctcaagatg
     1081 tcaccgccaa gatccgggca caagagggca agggaaacct cacattcgac tcggaatccg
     1141 gccgtatcgt ctccagccgc gttagtcaga aaatggagat ggtcatctcc gatcgcggtc
     1201 aagaccttgt tcaaacgacg gagacgacct cgacgatgat gctcgaccgt taggctctaa
     1261 tgtcggtctg cgcttgagtc aacgtgtcgg catcaaacga tgccgtgttt ctttactttg
     1321 ctgaaaaaag tgcttcgcgg catgcccaag agccgtgccg cgacgctttt gtttcccttg
     1381 gcttcgttca gcgcatcgat gagccgttgg cgttcgtagg ccacgaactc ggaattccaa
     1441 tcttcgccgg gcaacggttc cgaccccgaa ttggcccaga tggaatccgg aaactggtcg
     1501 cgaggaacgg gattccggct ccgaacatcg gttgtcccga tggacgtggg accatggggg
     1561 ctgacagcgc gcgacgcggc catcgtggga agccgcgggc ggagtcggcg acgaacaggc
     1621 tgccgcaatt ccggcggcaa atcgtcaaac atcaccgccg gcccgtcggc cagaacgacg
     1681 gcacgctcca ggacattttc cagctcccgg atattgccgg gccagtcgta agccaccaga
     1741 gcctcaacgg cctcgggatc gatgtgtgcg accagcttgc cggttcgttg cgcgtgaaga
     1801 ttgaggaacc aaaccgccag ctcgaaaacg tcctccctgc gctcgcgcag tggcggagtt
     1861 tccaggcaaa tcacattgag tcggtagtaa aggtcttcac gaaagcggcc ggcacgtatc
     1921 aaagcctcga ggtcttgatg agtcgcggcc aggattcgga cgtcaaccgt gatcggctgc
     1981 gagctgccca cgcgctcgaa tgacatttcc tgcaggacac gcagcagctt cgtctgaacc
     2041 tcgagattga tgtcgccaat ttcgtcaaga aagagcgtgc cgccgttggc ctgctcgaac
     2101 cgcccgacgc gatcgcgatc agcgccggta aatgcactct tgacgtggcc gaacagttca
     2161 ctttccagca ggctctgcga taaggcggca caatgcagct tgacgaaagg cccgtccgcg
     2221 cgggggctgg cttggtggat cgccgcggcc aggagttctt tgcccgtgcc gctctcgccc
     2281 cggatcaaaa ccgccgagtg ggtcactgcg atcttgcgag ccatggcgat catcttgccg
     2341 acggccgcgc tgacgcctcg aatcgactcg aaaggcccga gatcgccctc gcggtttgac
     2401 tcgtccgcgg gattgccaag cggtctttca ggcgacggac ccgagccgtc tcgttcacgc
     2461 tcggcttgct ctctgagctg atcctgcaag atcaagatcc gccgcttctg ttcggcgatc
     2521 ttttcgacct tgtcgcgaag ctcttgattc aagctttcca gggtctgctg gatatcggcc
     2581 gagtgcagaa ccagagtcgc gaccgagctc aaagcaccga ggaacgccat ctcctcatct
     2641 tcgtagggca taccgctgcg cttggggccc aagatcagaa ggcctgacaa atgcccgtcg
     2701 tctcccagcg atgtcgcggc ctcgcccccc agcgcgatca tcgcgtcggt cgccgggtcg
     2761 gatccaccgt cggaggccag cgcgtgggaa aggcgaaccg cggacgtttg ccgcaatcga
     2821 acagccagcg gattatcgga tgtgagcgac cgttcttccg gcgcggggcc aagactcgcc
     2881 accagttgca gcggccgacc gggcgcgtcc ccaaggtaga gagcgcccca ctccagacgc
     2941 aaaacctcgg cagcggcctc gagaagccgc cgacccagcg tcacccggtc gatcaaactc
     3001 cccaccgcaa gccgcatctt gtgcatggct cgatcgaact tgtacttctc ccggaaaaac
     3061 tggcggtcta tcacccgctg aacacgaccg cgagcgactt cagaaaggat gagcaccaca
     3121 atcactgaaa gggccgccgc caccgcacca agccaggtag agtgagtcga gaacgattga
     3181 tcgccgatca atttgccgct gaccaagagt acgccggaat aaatcagccc tgccgtcaag
     3241 ctaaaagcga agtaaaccgc tctgcggttg atgatttcct cgacctgcat cagcttataa
     3301 cgggtaatgc tgaaagcata tgccatcgta taaaggagtg atacgccaaa catcggccag
     3361 gctgcattgc ctcgtcccag cgtcgctgga tccatccaag cttgtgcaag caagtaagca
     3421 atcaagacca gcgaaatcag cgaagccagc aagatccact ggacctggtt gcgctccgcg
     3481 cgcgacggcg cattgcggta gctggaggtc aggcacagga tgcaaaggcc gaagagaaac
     3541 gcggccagaa cgacgtagcc aagggcaagc ctgcgcacca tttgaaacgc gagggcggtc
     3601 tgccagtagt ccttgttgag cgccgaccaa cgtgccgcat acatgctccc ccatagcgcc
     3661 agcaagtagg ccgtcggtat accgtatagt gcccccaaga cccactttcg gcgtcgcagc
     3721 aggatgggat ttgctcgggg gaaaatgaga aagaaatgaa gattcacaac cggcacgaaa
     3781 acggcgaaaa gcgcgaatgg gtaaatcagg agaggttcgg tgacaatctc agtccagtgg
     3841 tagcctccca tgaaggcacc gaccgtcacg atacatacgg caaagaacaa ctgcgccgag
     3901 ttgtcgtcag gccgtttcca aaaaacccgc gctccgatgg caaagatcag taattcctgg
     3961 agaaaccaga cgcacgagcg gtaataggtc caggaagggg ggtattgcac cttgaccgag
     4021 gccgagcgga tctcattcga atgctgatcg cgccatcgaa cttcgaccgt ctcgccaacc
     4081 tgagcactta gaccgcgcag cgccctgatg taatcagaat aacttcgctc gtgaatatcg
     4141 gaagaaccga tggtcagcaa cgtgtcgcca atccgtggcc gatcatcgtg ccaaatgtaa
     4201 tcggcggcaa tctcctcttc gaccttggtt ccgaacatgc agcgcacgcc aatggagccc
     4261 atacgggcga cgtgcgtcag cactgcgatt gaatacaaaa ggacgcccag ggagcatccc
     4321 atccacagca cgcgcagcat cgtccgccac caggatgatc ccagcgtcga aaagcccgtc
     4381 ggtcgccgac gcacacgtcc tcgaatgtcc ccgttcgaaa cgctgggcaa attcgggggt
     4441 ttagtccggc tcaacccacc gcgctgattc tgaggcaaat ccatggtgcc atcgctaaga
     4501 ggatgcggtc ccagagcggc aatgtccgat cgatgacctc attgtctgac gctctgaccg
     4561 cccttgtcta cactaccgtc tcggcgccga gacgcaagga tgtcgttagc gaaacttcgt
     4621 tattcctacc cactgatcgc ctctcggcga tttcacgagg attatttcca accacactta
     4681 tttgcacttt agacgatccg tgttcgacga gagcgcgcag actagggggc ttattccaac
     4741 aacct
//
LOCUS       DATAQL010001287        10883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3338806, whole genome shotgun sequence.
ACCESSION   DATAQL010001287 DATAQL010000000
VERSION     DATAQL010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338806"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..394)
                     /locus_tag="VHS97_22170"
     CDS             complement(107..394)
                     /locus_tag="VHS97_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3450976.1"
                     /translation="MPSRTEILDVISTVLARDLGLQASSAIDEHTRFFADLGLASIDA
                     VVLGERLQEHYGCPLPFNELMAEMGRRTQRDLSIGELVEFLAANLASANDS"
     gene            complement(400..5481)
                     /locus_tag="VHS97_22175"
     CDS             complement(400..5481)
                     /locus_tag="VHS97_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3450977.1"
                     /translation="MKRGRPYEIAIIGMGCRFAGASDLSAYFENILEARDCTRDVPRD
                     RWDAATFCDSHSQANDRVPSCRGGYLESPISFDAAAHGIMPRTVEGGEPEQFLVLDAA
                     AAAIADAGLTVGGLDQNRVEVVIGRGNYFNRGNLTRLQHGRMIAQTTSILEALHPEWS
                     QADRAAVRADLRASLPPFEAATIPGQLTNATAGRLANRLDLTGASFVVDAASASSLVA
                     LDLAARSLIEGRADLALAGGVYLEADVDFPLVFRQLNALSRSGTLRPFAAEADGMLSG
                     EGVGVVVLKRLADAERDLDRIYAVVQGIGLASDGKNQGLAAPSAHGHARAMRRAYRRA
                     RIDPATVMLVEGHGLGVPAADRAELRALKAVFPAPLQGKRALGAVSSMIGHAMPAAGI
                     AGVIKTALSLYHRVLPPTLHADKPHPLLEDPAGPFALNPAARPWIHADLETPRRAGVN
                     AFGFAGINAHAVLEEHAASADGHGKGALNRWDTEAILFSASDRSHLIDQTRELLGRLK
                     AKSPYTLLDVAYSQNSHCDHAPAGARLALVASSTADLAERLAALLPRLADPACRSIRD
                     GRGVYYWEEPLLDGGAGGLAFLFPGEGSQYPGMLADLCIHFPEVRRLFDTADRIARDL
                     GETVPPSEHLFGRAPAGDEKLWSAATAVNIVLNAQWALYQVLIRLGIEPEAVLGHSSG
                     ELLALSAAGVFETDRALERKLGRLGAIMSGFESLGDLPAARLVAVAAHRDRVEALCRD
                     VGAAGIDVAIDNCPHQVVLAVPSAELECVLDRLRQQSILWEVLPFSRAYHTPSFRPVV
                     GPIAAFFDQMSFRCPRVPIYSCASRARMPSDPGAIKELAIAQWTQTVAFRETIEAMHA
                     DGLRLFVDVGARGNLAGFVEDTLRGKPAFAIAANLPRRSGLTQLNHLVAATFAQGASL
                     KTDYLYARRSPRGIDWNAPKPSPHAFVELKIGFPEMHLSASLVERLRSDAEPPPCPDA
                     HEPAPALKDSWIETIDRGESAHRNGNGFAREDHPVAAWPQRRRRAPLAVESIDRPEAT
                     SRPPQNGRFEHEPVGQSDAITSAVDKAMLSFHQTMRAFLDTQQEVIAAYLGAPTGDHF
                     QTTAQLAETSLRDHSLLSFTHGNGIDASLTATKGPWAGEVRKLVAGSEVETLLVLEAH
                     DDPIAQHHTLGGRKISAVDPSLLGLSVVPFAVMAEMTAQAAALLVDSGQTLIGLKQVR
                     AHRWVRYEEQPVYLELRGHRLASSDDDRVWVGIFNRGTDGKADASRPVFESIAVFGKA
                     IPTPPPPAAWSLADPRPSRFTAFSVYDEQWLFHGPFFQAIARMGQISRHGIEGGIRVL
                     PLEPLLKRGQDAIFHTDLIVIDNFTQLLGAWGLDYLADGDVMFPLRMGDLEIYGKRPA
                     VGTEVACRISIDELQRHRVRVRAEFIRPDGTVWMRIHDWEDWRFHWPGRYRDAFRQPR
                     DYMVGEELPLDGQTTGAKAVWLEPPADMGRPVWRDVLEHTQLGPMERATYLTEPGPDQ
                     RRSQRLWGRIAAKEAARRLWKEAGDPATYPADLAIVAGEHGRPLLTRLGDYQLESTPA
                     ISIAHTEGIAVALAAADPDARVGIDVEPIVDRDASFLSAAFTPGERSLLGRRSGSNRA
                     EWTARFWCAKEAAAKASGLGLVGGPSSAEIVAVDDDTGAIDVRLGPKLEPAWPKDRRP
                     NPLRVMSARRGQHVWGWTITTGAKS"
     gene            complement(5478..10859)
                     /locus_tag="VHS97_22180"
     CDS             complement(5478..10859)
                     /locus_tag="VHS97_22180"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3450978.1"
                     /translation="MLGQVAALRSQTTTVADLHEEICARSTDFLDRTPPQVEPDGNVA
                     SRPQPSDIAIIGMSAVFPRAASVSQFWSNTLRGVDAITEVPPDRWDWRLYYDPDPKAP
                     DKIVSKWGGFLPDVAFDPLRYGMPPSSLPSIEPAQLLALEVVQTALADAGYAERPFRR
                     EQTAVVLGMGGGAAQLAMGYAFRSYLPMLDTVLPSGGKQAIASCQGLLPEWTEDSFPG
                     FLLNVTAGRIANRLNLGGANYTVDAACGSSLAAASLAVRELETGAADMVILGGVDTVQ
                     NPFTYLAFSKTQAFSPRGRCRPFDSSADGIVISEGVAAIVLKRLADAERDGDRIYAVI
                     KGVGASSDGRARGLTAPVVEGQTRALERAYHKAGVSPATIGYIEAHGTGTALGDVVEV
                     EALGELFRRAGANRRECALGSVKSLIGHTKCAAGLAGLINASLALYHKVLPPTIGIET
                     PNHKLDLRDGPFRLCKETQPWLHPYSDRPRRAGVSAFGFGGTNFHAVLEAYDRALVEV
                     PEQTMTDWPCELLVWQADESLQLMDQLDQLAAALDAGARPALRDLSHTLIRATQKRAA
                     RNSNARGATLAIVAASHTDLRNKLKLARKAIAEGRPSLDDPQGVVFEATPAWTGEKIA
                     FVFPGQGAQSTGMLRELAFIFPSVRNAFEEFDRVLLAGGGPPVGPLIFAPPAFGAAEE
                     EEARRALMPTDVAQPALGAASVAMLRLLAQLGCEPDLLGGHSYGELVALHAAGVLSAT
                     ALAELSLARGRLLRDAAKDRAGAMAALMAGPDDVERLVRDVPGVQTANWNGPRQTVIA
                     GPTAAVKQAVELAAAGGIQGMLLPVSCAFHTPMVASAREPLAQRARQLLTQAPRRPVY
                     SNLDARSHPEDPAAIAARLGEHLASPVRFADMITTMYADGARVFVEVGPGSILSPLIG
                     TILGERSHLAVACDRPGSPGLAGWLRAVGRLVAAGVPLCLEPLTAGRSPRVLDIQHLQ
                     AGDNIDLTPSTWMVNGSRARPANAPEPKRLGQALSRPETKMKNSTPPTGSSDRVIESF
                     QQTMQAFLEVQRSTMLAYLAGRGTAATTQDHAPHNAPSIGEVPAARSDVDSSSHSMNR
                     NGSAIEPSERSYGHKKSESNGHHHESNGNPGIQNGKAADSHASKSPAGGPDRTEITAR
                     LLETVRDRTGYPIETLGLDLDMEADLGIDSIKRVEILGKLRDEFPGLKALSDSPEMMD
                     AMARARTLGVIVERMTSLAQPKSGLVSAPANLHSAVLIATGTNGSGTANGKHESATQR
                     RLLEVVDAPLPRATSGLMAGGRMVITDDGRGLARILERRFQGAGIPVERLGGHDDPVD
                     WTSPASIDAGLEQLRSRGPLAGIVHALSLGSAVPVERIDSDWSARVGVEVRGLFLAAK
                     AMAADLENAAQAGGASLIAATALGGRYASAGSQASGFFPGHGGIAGLVKTLAREWPLI
                     RSRVVDFSPALPMETIATHLATEVFAGDGWAEVGYDQGRRIRLRTFASALDRNGSTME
                     LSPGEPVLITGGARGITALAAAELARLWRPTLLIVGTTPLPGDGEPADTLGLSAEADI
                     KVALLARLRRAGRPCSPAEIEKHYLALRRGREVRENLESLRQAGATVEYAQADVRDQA
                     ALAHVLDAWRARHGEIVALIHGAGLIKDKLIRQKSVESFDRVLETKLEGALNLIRLLR
                     GDALKFTALYSSIAGRFGNVGQSDYAAANEILNKLAHWLDRRWPGRVVSIIWGPWSGV
                     GMVAELESHLGRRGLGMISSEAGGSLLIDELRHGRKGDVEVIYSGKLGTLEQPILFAA
                     APECAEAFS"
BASE COUNT         1983 a         3564 c         3481 g         1855 t
ORIGIN      
        1 gtgacaccgt gaatcaagat cacgtctggc ccggagccag cctgctggta gtgaaagttc
       61 aatccgttcg ccttgagttt cggcatgaga gaagtctcgc ctgatattat gaatcgtttg
      121 cgcttgcaag attcgcggcc agaaactcga ccagctcgcc gatcgagagg tcgcgctggg
      181 tccttcgacc catctccgcc atcagctcgt tgaacggcag agggcagccg taatgttcct
      241 gaagtctttc gcccaggacg accgcgtcga tcgacgccaa gccgaggtcg gcgaagaaac
      301 gggtgtgctc atcgatcgcg cttgatgctt gcaacccaag gtctctggcg aggaccgtgg
      361 agatgacgtc gaggatctct gtccgagaag gcacggctat cacgatttcg ctcctgttgt
      421 gatcgtccag ccccaaacgt gctggccgcg ccgggccgac atcacgcgca gtggattcgg
      481 acgtctatcc tttggccaag ctggctcgag ctttgggcca aggcgaacat caatcgcacc
      541 tgtgtcgtcg tctaccgcga caatttcagc cgatgacgga ccacctacca accccagtcc
      601 cgaggccttg gccgcggctt ccttggcgca ccagaaccga gccgtccact cagcgcgatt
      661 cgagcccgac cggcgaccca agagagagcg ctcgcccggc gtgaatgcgg ccgacagaaa
      721 gctggcatcg cggtcgacga tcggttccac gtcgataccc actcgggcgt ctgggtctgc
      781 agcggccagg gccacggcga tcccctcggt gtgagcaatc gatatggcgg gcgtcgattc
      841 aagctgataa tcacctaatc gcgtcagcag gggtcggccg tgctcgccgg ccacgatcgc
      901 caggtccgcc ggatacgtcg ccggatcgcc cgcctccttc cagagtcggc gggcggcctc
      961 cttggcggcg atccggcccc agagccgctg cgaccgccgc tggtccggac ctggctcggt
     1021 caggtacgtc gcgcgttcca tcggcccgag ctgcgtgtgc tccaggacgt cgcgccacac
     1081 aggccggccc atgtcggcag gcggctcgag ccaaacagct ttcgccccgg ttgtctggcc
     1141 gtcgagcggc agttcctcac cgaccatgta atcacgaggc tggcggaatg cgtcgcggta
     1201 gcgccccggc cagtgaaacc gccagtcctc ccagtcgtgg atgcgcatcc agaccgtccc
     1261 atcgggccgg ataaactcgg cacgcacgcg cacacgatgc cgctggagtt cgtcgatcga
     1321 aatccggcac gccacctcgg tgccgacggc cggacgcttg ccatagatct caagatcgcc
     1381 catccgcagg ggaaacatca cgtccccgtc cgcgagatag tcgagacccc atgcgcccag
     1441 caactgggtg aagttatcga tgacgatcag gtcggtatgg aatatagcgt cctgccccct
     1501 ttttagtaag ggctcgagcg ggagcacgcg tattccaccc tcgatgccgt gccgtgagat
     1561 ttggcccatg cgggcgatgg cctggaagaa gggaccgtgg aagagccact gctcgtcata
     1621 caccgaaaaa gccgtgaacc tgctgggacg gggatcggca agcgaccagg ccgccggcgg
     1681 gggcggcgtg ggtatcgcct tgccaaagac cgcaatcgac tcgaacactg gtctggacgc
     1741 gtccgccttg ccatcggtcc cccgattgaa gatcccgacc cagacgcgat cgtcgtccga
     1801 tgacgccagc cgatgccctc gcagctcgag gtagaccggc tgctcctcat agcgcaccca
     1861 ccggtgggca cgaacctgtt tcaagccgat caaggtctgc cctgagtcga ccaagagcgc
     1921 agccgcctgc gcggtcatct cagccatcac cgcaaacgga acgaccgaca atcccaagag
     1981 cgacggatcg acggccgaga ttttgcgtcc ccccaaggtg tggtgctgcg cgatgggatc
     2041 atcatgggcc tcaaggacca ggagtgtttc gacctccgag ccggcgacca gcttgcgaac
     2101 ttcgcccgcc cagggaccct tggttgcggt tagggatgcg tcgataccgt tcccgtgtgt
     2161 aaacgaaagt aaagaatggt cgcgaaggga tgtctccgcg agctgcgcgg ttgtctggaa
     2221 atggtcccca gtcggcgcac ccagataggc cgcgatgact tcctgttgag tgtccaagaa
     2281 cgcccgcatc gtttggtgga acgagagcat ggccttgtcg acggccgatg tgattgcgtc
     2341 actctggccc actggctcgt gctcgaagcg gccgttttgt ggaggccggc tggtcgcttc
     2401 cggccgatcg atggattcga cggccagagg agcgcggcga cggcgctggg gccatgccgc
     2461 gaccggatgg tcttcgcgcg cgaacccgtt gccgttgcga tgtgcgctct cccctcgatc
     2521 gatcgtttct atccaggagt ctttcaatgc tggtgcgggt tcgtgcgcgt ctggacacgg
     2581 cggaggctcc gcatccgacc gcaagcgctc gaccaacgat gccgacaggt gcatttccgg
     2641 aaagccgatc ttgagctcga cgaaggcgtg gggcgatggc ttcggggcgt tccaatcgat
     2701 accgcgaggc gatcgccgtg catagagata atcggttttg agcgatgccc cctgcgcgaa
     2761 cgtcgctgcg acaagatgat tcaactgggt caaaccgctc cggcgtggga ggttcgctgc
     2821 tatcgcaaag gccggcttac cgcgcagcgt gtcttcgacg aatccggcca ggttgccccg
     2881 cgcccccacg tcgacaaaca gccgcaggcc atcggcatgc atggcctcga tggtctcacg
     2941 aaacgccacg gtctgggtcc actgcgcgat cgccagctct ttgatcgcgc cgggatcgct
     3001 cggcatccgc gcacgcgatg cgcacgaata gatcggcacg cgcgggcagc gaaagctcat
     3061 ttgatcgaag aacgcggcga tcgggccgac gaccggtcgg aagctgggcg tgtggtacgc
     3121 acgcgaaaac ggcagcacct cccagaggat gctttgctgg cgtaagcgat cgagaacgca
     3181 ctcgagctcg gccgagggga cggccagaac gacctgatga gggcagttgt cgatcgcgac
     3241 atcgatacct gccgccccga cgtcgcggca aagagcctcg acgcgatccc gatgggcggc
     3301 cacggcgacc aaacgagccg ccggcaaatc gcccaacgac tcgaaaccgc tcatgatcgc
     3361 gcccaatctg ccgagcttgc gctcgagcgc ccgatcggtt tcgaacacac ccgccgcgga
     3421 gagtgccagc agttcaccgc tgctgtggcc gagcacggcc tcgggctcga tccccagccg
     3481 gatcaagact tgataaagcg cccattgggc gttgagtaca atattcactg ccgttgcggc
     3541 cgaccagagc ttttcatcgc cggcgggtgc gcggccaaac aggtgctcgc tcggaggcac
     3601 ggtttcaccg aggtcgcggg cgatccgatc ggccgtatcg aaaaggcgac gaacttcggg
     3661 aaagtggatg cacagatcgg caagcatccc cggatattgc gagccctcgc ccggaaagag
     3721 gaaagccagg cctcccgcgc cgccgtccaa gagcggttct tcccagtagt aaacgcctcg
     3781 gccgtcgcga atcgaccggc aagccgggtc cgccaggcgg ggcagcaagg ccgccagccg
     3841 ctcagccaga tctgcggtcg aagaagccac cagggcgagg cgcgcgccgg ctggcgcatg
     3901 gtcgcagtgg ctgttctggg aataggccac gtccaaaagg gtgtacggcg acttggcctt
     3961 gagtcggccg agcagttcgc gcgtctggtc gatcaaatgc gatcgatcgc ttgccgagaa
     4021 aaggatggct tccgtgtccc agcgattgag cgcgcctttg ccgtggccgt cggcagaagc
     4081 cgcgtgctct tcgagaacgg cgtgcgcatt aattccggcg aagccaaacg cgttgacccc
     4141 ggcccgtcga ggtgtctcga gatcggcgtg aatccagggc cgggcggccg ggttgagcgc
     4201 gaacggaccg gcgggatctt cgagcagcgg atgcggtttg tcggcgtgca aggtgggcgg
     4261 caagacgcgg tggtagagcg agagcgccgt cttgatcacc ccggcgatgc cggcggccgg
     4321 catggcatga ccgatcatgg atgagaccgc acccagcgca cgcttgcctt ggagcggggc
     4381 ggggaaaacc gcttttagtg cgcgcagctc agcccgatcc gcagcgggaa cgcccaggcc
     4441 gtggccttcg accagcatca cagtagcagg gtcgattctc gccctgcgat acgctcgccg
     4501 catcgcgcgg gcgtgcccgt gcgctgaggg cgcagccaac ccttggtttt tgccgtcgct
     4561 ggccaggccg atgccctgga cgacggcgta gatccgatct aggtcccgct cggcgtccgc
     4621 gagacgtttt agaacgacga ctcccactcc ttctcccgag agcataccgt cggcttcggc
     4681 cgcgaacggc cggagtgttc ctgagcgcga cagggcattg agctggcgaa agaccaaggg
     4741 aaaatccacg tcggcctcga gatagacacc gcccgcaagg gcgagatcgg cgcgtccttc
     4801 gatcagtgac cgagccgcga ggtcgagcgc gaccaaggaa gaggcgcttg ccgcgtcgac
     4861 gacgaagctg gctccggtca ggtcgagccg gttggcgagc cgaccggctg tggcattggt
     4921 caattgaccg ggaatcgtcg ccgcctcgaa cgggggtaaa ctggctcgca gatccgctcg
     4981 cacggctgcc cggtcggcct gcgaccactc gggatgaagg gcttccagaa tcgaagtcgt
     5041 ctgcgcgatc atccggccgt gctgcagccg agtcaggttc ccacgattga aataattgcc
     5101 ccggccgatc acgacctcga ctcgattctg gtcgagcccg cccacggtca gtccggcatc
     5161 ggcgatcgcg gcggcagccg cgtccaacac caggaattgt tcgggctcgc ccccttcgac
     5221 cgtacgcggc atgatcccgt gtgcggccgc atcgaacgaa atcggcgatt ccaggtaacc
     5281 accccggcac gagggcacgc gatcgttagc ttgcgagtgc gagtcgcaaa aggtcgcggc
     5341 gtcccagcga tcgcgcggaa cgtcccgcgt gcagtcccgc gcctccagga tgttttcaaa
     5401 gtaagcgctc aggtccgatg cgccggcaaa gcggcaaccc atgccgatga tggcgatctc
     5461 gtagggacgt cctctcttca tgagaacgcc tccgcacact cgggagccgc cgcaaagagt
     5521 atcggttgtt cgagagttcc cagcttgccc gagtaaataa cctccacgtc acctttacgg
     5581 ccgtggcgca attcgtcaat caaaagtgag ccgcctgcct ccgacgagat catgccgaga
     5641 ccgcgccgcc cgagatgact ctcgagctcc gcgaccatcc ccactcccga ccaaggtccc
     5701 catatgatcg acacgacgcg gcccggccag cgtcggtcca gccagtgagc cagtttgttc
     5761 aagatctcat tggcggcggc gtaatccgac tggcccacat tgccaaatcg gcctgcgatc
     5821 gatgaataaa gcgccgtgaa cttgagcgcg tcgccgcgca agagccggat caggttgagc
     5881 gctccttcga gcttggtttc gagcacacgg tcaaacgatt cgaccgattt ctggcgaatg
     5941 agcttgtcct tgatcaaacc ggcgccgtgt atcaaggcca cgatttcgcc atggcgggcg
     6001 cgccaggcat cgagcacgtg tgccagggcc gcttggtcgc gcacgtccgc ctgggcatat
     6061 tcgacggtcg cgccggcctg ccgtaagctc tccaggtttt cgcgaacttc acggccgcgg
     6121 cggagcgcca ggtagtgttt ttcgatttcg gccggactgc acggccgccc tgcgcggcgc
     6181 aaccgcgcca agagcgcaac tttgatgtcg gcctcggcgc tcaagcccag cgtatcggcg
     6241 ggttcgccgt caccgggcaa cggggtggta ccaacgatca gaagggttgg ccgccagagc
     6301 cgcgccagct cggccgccgc cagggccgta attccgcggg caccccccgt gatcaacacg
     6361 ggctcgcccg gactgagctc catcgtggaa ccgttgcgat cgagcgcact ggcgaaggtc
     6421 cgcagtcgga ttcgccggcc ctggtcgtag ccgacctcgg cccagccgtc gccggcgaac
     6481 acttccgtgg ccaggtgggt ggcaatcgtc tccatcggta gggcgggtga aaaatcaacc
     6541 acacgcgaac gaatcaaagg ccattctcga gccaacgttt tgaccagacc ggcaatgccg
     6601 ccgtgtccgg ggaagaatcc ggaggcctgg ctgccggcgc ttgcatatcg ccccccaagt
     6661 gcagttgccg cgatcaagct cgctccgccc gcctgagcag cgttttccag atcggcggcc
     6721 atggccttgg ccgccaaaaa caggcctctt acctcgacac caacccgcgc cgaccagtcg
     6781 gagtcgattc gttcgaccgg gaccgcactc ccgagcgaaa gcgcgtgcac gatgccggcc
     6841 aacggaccgc gcgaccgcaa ttgttcgagc cccgcatcga tagacgccgg cgacgtccag
     6901 tcgaccggat catcgtggcc acccagccgc tcgaccggga ttccggcccc ctggaaacga
     6961 cgctcgagta tgcgggccaa gccgcgtcca tcatcggtga tcaccattcg gccccccgcc
     7021 atcaggccgg acgttgcacg gggcaggggg gcatcgacta cttccaaaag ccgccgctgg
     7081 gtcgcgcttt cgtgcttgcc attggcagtt ccgcttccgt tagttcctgt cgcgatcaac
     7141 accgccgagt ggagatttgc cggcgcgctg actaaaccgc tcttgggctg ggccaggctc
     7201 gtcattcgct cgacaatcac gcccaaagtg cgagcccggg ccatcgcgtc catcatctcc
     7261 ggcgaatcgg acagggcctt caatcccgga aactcatcgc gcagcttccc cagaatctcg
     7321 acccgtttga tcgagtcgat ccccaggtcg gcttccatgt cgaggtcaag ccccagggtc
     7381 tcgatggggt agcccgtacg atctcgaaca gtttcgagca gccgggcggt gatctccgtt
     7441 cgatccggtc cgccggccgg ggacttcgac gcgtgcgaat ccgcagcctt gccgttctgg
     7501 atcccagggt ttccgtttga ctcgtggtga tgcccgttgc tttcgctctt tttgtgcccg
     7561 tacgagcgtt cgcttggctc tatcgcggac ccgtttctgt tcatgctgtg tgaggacgag
     7621 tcaacgtccg agcgcgcggc cgggacctcg cctatggagg gcgcattgtg cggagcgtga
     7681 tcctgggtcg tagcggccgt gccacgcccg gcgagatacg ccagcatcgt cgacctctgg
     7741 acctcgagaa acgcctgcat ggtctgctgg aacgactcga tgacacggtc gctcgagccc
     7801 gtgggggggg ttgagttttt catttttgtt tccggtcgag aaagggcctg gcccagccgt
     7861 ttgggctcgg gtgcgtttgc tggccgagct cgactgccgt tgaccatcca agtcgacggc
     7921 gtcaggtcga tattgtcacc cgcctggagg tgctggatgt cgaggacgcg cggcgagcgg
     7981 cccgcggtga gcggctcgag acaaagcggc acgccggccg ccaccagccg accgacggcg
     8041 cggagccagc cggccagacc gggcgaccct gggcgatcac atgcgacggc aagatgcgac
     8101 cgctcgccca ggatggtgcc gatcaaggga gaaaggattg agcccggtcc gacctccaca
     8161 aacacgcgag ccccatcggc gtacatcgtt gtgatcatgt cggcgaatcg taccgggctg
     8221 gcaagatgct cgcccagccg cgccgcgatc gctgctgggt cctccggatg ggagcgggca
     8281 tcgagattgg agtaaaccgg tcggcgggga gcctgggtga gcaactggcg tgcccgctgt
     8341 gctaacggtt cgcgtgcgga cgccaccatc ggcgtgtgga atgcgcacga gaccggcaag
     8401 agcatgccct gaatgccgcc ggccgcggcc agctccaccg cctgtttcac cgccgcggtc
     8461 ggcccggcaa tcaccgtctg cctgggacca ttccagttag cggtttgcac gcccggcacg
     8521 tcgcgaacga gccgctcgac gtcatccggt ccggccataa gcgccgccat ggcgccggcg
     8581 cgatccttgg ccgcatcccg caggaggcgg ccacgggcca gcgagagctc ggccagggcc
     8641 gttgcgctca aaacacccgc tgcatgcagc gccaccagct caccgtagct gtggccgccc
     8701 aacagatcgg gctcgcaacc cagttgcgcg agcaggcgga gcatagcgac gctggcggct
     8761 cccaacgccg gctgggcaac atcggtgggc atcaacgccc gccgcgcctc ttcctcctcg
     8821 gcggcgccaa aggccggcgg cgcgaagatg agcgggccca ccggagggcc accaccagcg
     8881 agcagcacac gatcgaattc ttcaaaagcg ttgcggactg aggggaagat gaacgccaac
     8941 tcgcggagca tcccggtcga ctgcgcaccc tggccgggga agacgaacgc gattttctcg
     9001 ccggtccagg ccggcgtggc ctcgaaaacc acgccctgag gatcgtcgag gctgggccgc
     9061 ccttcggcga tcgccttacg ggccagcttt agcttgttgc gcaaatcggt gtgagacgcg
     9121 gccacgatcg ccagcgtcgc accgcgtgca tttgagttcc gcgctgcccg cttttgcgtt
     9181 gcgcggatca gcgtgtgcga caaatcccgc agcgccgggc gagcgcccgc gtctagcgcc
     9241 gcagccagtt gatcgagttg atccatgagt tgaagcgatt cgtcggcttg ccataccaac
     9301 aattcgcagg gccaatcggt catcgtttgc tcgggaactt cgaccagtgc gcgatcgtac
     9361 gcctcgagga cggcatggaa attggtgccg ccaaatccga aggcgctgac cccagctctg
     9421 cgcggccggt cgctgtaagg gtgaagccag ggctgcgtct ctttacagag ccggaacggc
     9481 ccatcgcgca ggtcgagctt gtggttaggc gtctcgatgc cgatcgtggg gggaagcacc
     9541 ttatgataaa gagccaagga tgcattgatc agtccggcca gaccggcggc gcacttggta
     9601 tgaccgatca gcgacttcac cgagcccagc gcgcattcgc gcctgttggc tccggctcgc
     9661 ctgaagagct ctccgagcgc ctcgacttca accacgtcgc ccagggcagt gccggtcccg
     9721 tgtgcttcaa tatagccaat ggtcgccggc gagacgccgg ctttgtgata cgctcgctcg
     9781 agtgcacgcg tctgcccttc gactaccggc gcggtcaggc cccgagcgcg gccgtcgctc
     9841 gaggcaccca cccctttgat caccgcatag atccggtcac cgtcgcgctc ggcgtcggcc
     9901 aggcgtttga gaacgatcgc ggcgaccccc tcgctgatga cgatcccatc ggcgctcgag
     9961 tcaaacgggc ggcagcggcc ccggggcgaa aaagcctggg tcttgctgaa ggccaaataa
    10021 gtaaacgggt tttggacggt gtccacccca cccaagatca ccatgtcggc ggcaccggtt
    10081 tcgagctcgc gcactgccag cgaggccgcc gccagcgacg aaccgcaggc ggcgtcaacg
    10141 gtgtagttgg cgccgcccag gttcagccgg ttggcgattc gacccgcggt aacgttgagc
    10201 aggaaacctg gaaacgagtc ttccgtccat tccggcagca acccctggca cgacgcgatt
    10261 gcctgctttc cccctgacgg caagacggtg tcgagcatgg gtaaatagga acggaacgca
    10321 tagcccatcg ccagctgcgc ggcccctccg cccatgccga gcaccaccgc tgtctgttca
    10381 cgacgaaacg gccgctcggc gtaaccggcg tccgcgagcg ctgtctggac cacctcaagc
    10441 gccagcagtt gagccggctc gatcgagggg aggctcgagg ggggcatccc gtaccgcaag
    10501 ggatcgaatg cgacgtcggg caagaatccg ccccacttcg acacgatttt gtcgggcgcc
    10561 ttcgggtccg gatcgtaata cagccgccaa tcccagcggt ccggcgggac ctcggtaatg
    10621 gcatcgacgc cccgcaacgt attggaccaa aactgcgaga cactggccgc gcgggggaaa
    10681 accgcggaca tcccgataat cgcgatgtcc gacggctgtg gccgggaggc gacgttgccg
    10741 tcgggttcga cctgtggggg agtgcggtcc aggaagtcag tactccgggc acaaatctcc
    10801 tcatggaggt cggcgaccgt cgtcgtctga ctccgtaggg cagcaacttg acccagcatg
    10861 tagaggccgt gggttgcctg ata
//
LOCUS       DATAQL010001288         7767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4100299, whole genome shotgun sequence.
ACCESSION   DATAQL010001288 DATAQL010000000
VERSION     DATAQL010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4100299"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..2681
                     /locus_tag="VHS97_22185"
     CDS             285..2681
                     /locus_tag="VHS97_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3450979.1"
                     /translation="MDRLAPRGWGLLTSLALIFWAPSALAQPTDKKLADYFGFQPLEL
                     YKLDNRIGNLHLVDLDGDKVDDIFVTNNGRSRIDLFLSSKKPADDNSSRPFRKDANEL
                     EYDRRMRLVSIPVNKAVVSVDTGDFNGDGKPDLVFYGTPAEVEILFNEGKGRFGSPKK
                     INTGDAIQRPAALAVGDFDQDGRDDLALLAEKELVFVYQTAAGIMGEPERVPHTAASP
                     WLIRAVDLDGDGAKDLVILDTESEHPIHVRFATDEKKLGPEQRFALEVPSAVAFGQVD
                     GKGGSEILVLEGQSGRAKVLTLDQSSDDDANRRGRLAFFGLPQGSERGRSLAVGDLDG
                     DHRKDVIVTDPANAQVWVYVQSGRAGLSAGLTFPSLGNARTVRLAHQESGGKDEVYVL
                     SEQEKQVGRSIFEKGRLSFPTPLSLTGEPVAMDVVDLDGDKAPEILYVSRAKAKTDKD
                     ADAFELRAVTRDKAGAYQPTKWGEVDSVALPDVKAVPAAVKTLDINQDGQMDLLIFKE
                     YGAPLLVLGEKGGPPRPFTGSLGPLSGVAPAGVSLANLDGPAVIVAQNTYARRVTLDA
                     DGHWNIKDQYNAGRNSAQIVGATALDTDGDGTKEIVMLDRATKSLLFLSMKDGVYRPS
                     GRLLIGTINFIGMSVADLDGDGRDDLLIAGTDRFAVLQTGRKGQRLKTIASYESKRTE
                     ARLTDLAIGDVNGDGLPDVVFSDVAEQSLEIATYAGDPELIPAITFKIFERKTFRNVG
                     DVIEPRDMAIGDVDGDGRADIVLVIHDRVVVLRQDAGKPGAKPEQPTVKSSTASRPAP
                     "
     gene            2789..4150
                     /locus_tag="VHS97_22190"
     CDS             2789..4150
                     /locus_tag="VHS97_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3450980.1"
                     /translation="MAKSAQGMHWPGYAGTATMLVLALLFPVGLMIFDPTLMFERGWE
                     QYVGTGIYFWAVVTLGRELVRIWRNEQAFEDAPLLLKRVNAALARVGPGDGFSATSVI
                     ADDDSRILPLRVRQLTSFLRESRSPSVTQLMEVNREGSGLDQEQMAGRFTLTRYILYL
                     LPVIGFIGTVEGISKALMNISKVLPMVKNLDGFLTNLTSVTAALQIAFDSTLLALFLS
                     AALMLVQTLVFRRSEDILARVDRWVVEHVLPHVGTDNPLAEQLTEVIGKQLDELGTKL
                     ARVLEPAVQALHEQTDKLGESLRVPIGQFTREMERLPDAFYSFKQGADTIGRIGADLE
                     AIGSASESLRRGVATLGRIETLLGQMPSSHAELEEIKRGLERAGASIDTLAGSWSAAY
                     ERSSRATQEQLARSLTSLKDALEMLNVSMEQGNSLYRTIVKKLFDERHEPGVHRPESI
                     KVA"
     gene            4178..4960
                     /locus_tag="VHS97_22195"
     CDS             4178..4960
                     /locus_tag="VHS97_22195"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEX3450981.1"
                     /translation="MRRSRRGGGSSSEFGGSGEDSFVAVVVTKLTGALLFILLLTMVI
                     MALLPKAVDLENEEKSTLKADVTEGLPLRIATPTRLPEAVAGRSYLLALAATGGRGPL
                     HWSVDEALPEWLRLDESTGQLAGTPPKATDKPLELDLRVSDGPQTASQPALLQVLPFQ
                     TPMTASSWLKQSLTIVPWRAWLEQGVGFLILWLVHLLGMNLLGNFERGSLEESVLAPD
                     HAAAQISVRKRFASYRLVVRLATLSAVMSLAVWLFLAMPRPH"
     gene            5188..6645
                     /locus_tag="VHS97_22200"
     CDS             5188..6645
                     /locus_tag="VHS97_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450982.1"
                     /translation="MSRLRITLISAAMMVALAALALNSLAQPPGRNGGGPGGFFGRGG
                     GPGGPGGSLLSLASNPAVQEDMKVKDKQKAQIKTLSDKFNVQSREIWTQMGGGPGGPG
                     GPGGGPPGGAPNAQGKGGRRQGQNGQGNGNAQADVQVAQGNGGFGGVDNGQIQDPNAP
                     GFDPNQGQVGRGQGGQGGGGRGNRGPGGQQDPGRAQQFAMMREAMDELTQSAEASLGK
                     ILDKTQAIRLKQIQLQLQGLGAILREDMMEKLGIDESQIQMLDEVRSNYRTAQRENGQ
                     ARRDVMKAVFAKAIPNQNNGQNADNAANGGNAGNNGNGNTANGNGGNGNGRNGGRGNR
                     GRPDPEAMKKAMEDPQIQAQMEQMNTQDQKLENQFSLAIAKVLTPRQRALYKKMLGAP
                     FDKSKMGTGGPWGGRGGNGPGGNPATAKAGATAGKTATATTNASADDDAVESSTTAKP
                     ATTTPAPAKTKAATTTPRKKSLRELRGSSSDSNDQ"
     gene            complement(7338..>7767)
                     /locus_tag="VHS97_22205"
     CDS             complement(7338..>7767)
                     /locus_tag="VHS97_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450983.1"
                     /translation="AGAYGSFVYDPTGTAWSFAGFSGVAANGSGFTAGNPPAPEGAQV
                     GVLQMTGSFSQSVAGWAGGSYFITFDAAQRGNYGGPAQDFEVLVDGTVVSPFTPSGTS
                     YQAYSTAAFTVAAGSHTITFQGLDSAGGDNTALIDAVAIS"
BASE COUNT         1626 a         2306 c         2436 g         1399 t
ORIGIN      
        1 aaacgactta caacaagccc ttttggttcg tgacggacat gacagactca agcaggtttt
       61 aaaccaattc tattttttcg cgtctgtcaa ggggcgcacg tcgcagggga cggcgtcagg
      121 accggcgcaa ttgtcaaatt gtgagcgcgc aatacgcagg agtcgagttc gaccgcgccg
      181 ttttcccttc ctcttgtcgt cgatacgggc tcttcgtacc ctgatgtttt ccgcgcgcca
      241 ttccgcgccg ggatctacgc ttacgcccaa tcaggatgga aatgatggat aggctagcgc
      301 ctcgcggttg gggactactt acttcactgg cgttgatttt ctgggcgccg tcggccctgg
      361 cccagcctac ggacaagaag ctcgccgatt acttcggatt ccaaccgctc gagctctaca
      421 agcttgataa ccggattggc aacctccatc ttgtggacct ggatggcgac aaagtcgatg
      481 acattttcgt gaccaacaac ggccggtcgc ggatcgacct cttcttgagt tccaagaagc
      541 ccgccgacga caatagttcg cgcccgttcc gtaaagatgc caacgagctc gagtacgacc
      601 gccggatgcg gctggtcagc atcccggtta acaaagcagt cgttagcgtc gacacggggg
      661 actttaacgg agatggtaag cccgatcttg ttttttatgg aacaccggcc gaggtcgaga
      721 tcctgttcaa tgagggaaag ggcagatttg gtagtcccaa gaaaatcaac accggcgatg
      781 caatacagag gccggcggcc ctggccgtgg gggacttcga ccaggatggt cgcgacgatc
      841 tcgccctcct ggcggagaag gagttggtct tcgtttatca gaccgccgca gggatcatgg
      901 gcgagccgga acgagtgccg cacaccgccg ccagcccatg gctgatcagg gcggtcgacc
      961 tggacggcga cggcgccaag gaccttgtca ttctcgacac tgaaagcgaa cacccgatac
     1021 atgtccggtt cgcgaccgac gagaaaaagc tcggccccga gcagcgcttc gcccttgagg
     1081 tgcccagtgc ggttgccttc ggccaggtgg acggcaaggg gggaagcgag atcttggtcc
     1141 tcgaggggca gtcgggccgt gccaaggttc tgacactgga ccaatcttcc gatgacgatg
     1201 ccaacagacg gggccggctg gcgttttttg gcctgccgca ggggtcggaa cgaggccggt
     1261 cactggcggt aggcgacctc gatggcgatc accgcaaaga cgtgatcgtc accgaccccg
     1321 ccaatgcgca ggtctgggtc tacgtccagt cagggcgagc ggggctcagc gcgggattga
     1381 cgttccccag cctgggcaat gcgcgcaccg tccgactggc gcaccaggaa tccggtggca
     1441 aggacgaggt ttacgtgctt tccgagcaag aaaagcaggt gggccgcagc attttcgaga
     1501 agggtcggtt gtcattcccc actccgctgt cgctgaccgg cgagcccgtc gcgatggacg
     1561 tggtcgacct cgatggcgac aaggcgccag agatccttta cgtgtcgcgc gccaaagcca
     1621 agacggacaa ggatgcggat gcttttgagc tccgagccgt aacgcgcgac aaagccggcg
     1681 cgtatcagcc aaccaagtgg ggcgaagttg actcggtcgc gctgccggac gtaaaggcgg
     1741 ttccagccgc cgtcaagact ctcgacatta accaagacgg gcagatggac ctcttgatct
     1801 tcaaggaata cggagcgccc ctcctcgtgc tgggggaaaa aggagggcct ccccggccat
     1861 tcaccggcag tctgggcccc ctctccggag tggccccagc gggagtaagt ctggcgaatc
     1921 tcgacggacc ggcggtgatc gtcgcccaga atacctacgc gcgacgggtc acactcgacg
     1981 ccgacggcca ctggaacatc aaggatcagt acaacgcagg ccgcaactcc gcccagattg
     2041 tcggcgccac cgccctggac accgacggcg acggcaccaa ggaaattgtc atgcttgacc
     2101 gtgccaccaa gtcgctcttg ttcctttcaa tgaaggatgg agtctacagg cccagtggca
     2161 ggctgttgat cggcactatc aacttcattg ggatgagcgt ggctgacttg gacggcgacg
     2221 gccgcgacga cctcctgatc gccggtacgg atcgattcgc cgtactccag acgggacgca
     2281 agggacaacg cctgaagacg atcgccagct acgaatcaaa acgcaccgaa gccaggctca
     2341 ctgacctggc gatcggcgac gttaacggcg atggattacc cgacgtcgtc ttttccgacg
     2401 tggcggaaca atccctcgag atcgcgacgt acgccggcga ccctgagctg attccggcga
     2461 tcaccttcaa gatcttcgag cgcaagactt tccgcaacgt cggcgacgtg atcgaaccgc
     2521 gcgacatggc aatcggcgac gtggacggcg atggacgagc cgacatcgtc ttggtcattc
     2581 acgaccgcgt tgtggtcctg cgtcaggacg ctggaaaacc gggagcgaag cccgaacagc
     2641 cgaccgtcaa gtccagcacg gcgtctcgcc ctgccccttg aatctctctt gacggaagag
     2701 ccgaacagag cgataccacc agggcaacgg aactaccggc ccgagccatc gctcgcgaca
     2761 gaccggatca ctcaggaggg agccagggat ggcgaaaagc gcgcaaggta tgcactggcc
     2821 aggatatgca ggcaccgcaa ctatgctggt cctggcgctg ctcttccccg tcggcctgat
     2881 gatcttcgat cccaccctga tgtttgagcg tggctgggag cagtacgtcg gtaccggcat
     2941 ctatttctgg gccgtcgtca cgcttggccg cgagctcgtc cgcatctggc gcaacgagca
     3001 agctttcgaa gatgcaccgc tattgcttaa aagagtaaac gcagccctgg cgcgcgtcgg
     3061 cccgggcgac ggtttctcgg ctaccagcgt cattgccgat gacgattccc gcatcctgcc
     3121 gctccgtgtt cggcagttga ccagtttctt gagggagagc cgcagcccat ctgtcactca
     3181 gctcatggaa gtcaaccgcg agggttccgg cctcgaccag gaacagatgg ccggtcggtt
     3241 cacgctcaca cgctatattc tgtatctctt gccagtgatt ggatttatcg gcacggtcga
     3301 gggcatctcg aaggcgctca tgaacatcag caaggtgctg cccatggtga aaaacctgga
     3361 cggcttcctg acgaacttga caagcgtgac ggccgcgctc cagatcgcgt tcgacagcac
     3421 cctgttagcc ctctttttga gcgcggcgtt gatgttggtt cagacgcttg tgttccggcg
     3481 ctcggaagac atcctcgcgc gggtcgaccg ctgggttgtg gagcacgttt tgccgcacgt
     3541 gggcaccgat aatcctctgg cggagcaatt gactgaggtg atcggtaagc aactcgacga
     3601 actgggaacc aaactcgcgc gggtgctcga gccggcggtc caggctcttc acgagcagac
     3661 cgacaagctt ggcgagagtc tgcgcgttcc gatcggacaa ttcaccaggg agatggagcg
     3721 tcttcccgat gccttctatt ccttcaagca gggtgccgac acgatcggac gcatcggagc
     3781 ggacctagag gcgatcggct cggcgagcga gtcgctgcgc cgcggggtgg ccacgctcgg
     3841 tcgcatcgag acattgctcg gtcagatgcc ctcgtcgcac gcggagctcg aggaaatcaa
     3901 gcgcggactg gagcgagccg gggcttcgat cgacacgctg gccggttcgt ggtcggcggc
     3961 gtacgagcgt agcagccgcg ccacgcaaga acagttggcg cggagtttga ccagcctcaa
     4021 ggatgccctg gaaatgttga atgtgagcat ggaacagggc aattcgctct atcgaaccat
     4081 tgtcaagaag ctattcgacg aacgacacga acccggcgtg catcgcccgg aatccatcaa
     4141 agtggcttga aacgaagcct gaagtggggg agtttgcatg cgacggtcac ggcgaggcgg
     4201 aggttcgagc tcggagtttg gcgggtcggg cgaggattcg tttgtcgcgg tcgtggtcac
     4261 caagctcacc ggggccctgc tctttatcct gctcctcacc atggtgatca tggctctcct
     4321 tccgaaggct gtcgacctgg agaacgagga gaagagtacg ctcaaggccg acgtgacaga
     4381 aggactgccg ctaagaattg cgactccaac gcgattgcct gaggctgttg ctggccgctc
     4441 ttacttgctc gccctcgccg ccacgggagg gcgtggcccg ctgcactggt cggtcgacga
     4501 ggcccttccg gaatggctca ggctggacga atccactggc cagcttgccg gcacgcctcc
     4561 caaagcgacg gacaaacccc tcgagctcga cctgcgcgtg agcgacggtc cccaaaccgc
     4621 cagtcaaccc gccctgctcc aagtcctgcc cttccagacg cctatgaccg ccagctcctg
     4681 gctcaagcag agcctgacta ttgtcccttg gagggcgtgg ttggaacaag gcgtcggttt
     4741 cctgatactc tggctggtgc acctgctggg aatgaatctc ctgggcaact tcgaacgcgg
     4801 ctccctcgaa gaatccgtgc tcgcaccgga tcatgcggcc gcgcaaatat ctgttcgcaa
     4861 acgatttgca tcctaccggc tggttgtcag actggccacg ttgagtgcgg ttatgtcgct
     4921 ggcagtctgg ctgttcttgg ccatgccacg cccgcactga tacaagtctc accaagcgag
     4981 atcctggcga tttattgcgg tcagtcgagt gggctcgcag tcgtttgcgc ctggtcggcg
     5041 gaatacgaat tgcatctttg acaaaaagtt atatggccaa cgtcagctcg attgccgcta
     5101 agatcattaa tatactggag cggtccccgt tgtccgaggg tcgttgcgcg actttgcgaa
     5161 gcccgcgtct gactttagga gttgatgatg tcacgactga gaatcacctt gattagcgcg
     5221 gcaatgatgg tcgcactggc tgcgctggct cttaactcgt tggctcagcc gcccggccgc
     5281 aacggcggtg gcccgggggg cttcttcggc cggggcggcg gccctggtgg tcccggcggc
     5341 tcgctgctgt cgctggcttc caacccggca gtccaagagg atatgaaggt caaggacaaa
     5401 cagaaagctc aaatcaagac tctcagcgat aagttcaacg tgcaatcgcg cgagatctgg
     5461 acccaaatgg gtggcggtcc cggaggtcct ggcggtcccg gtggtggtcc tcctggcggc
     5521 gcaccaaacg cccaaggcaa gggaggacgg cggcaaggcc aaaacggaca ggggaatgga
     5581 aacgcccagg cggacgtgca agttgcgcaa ggaaacggcg ggtttggggg cgttgacaac
     5641 ggccagatcc aggatccgaa cgccccgggc ttcgacccca accaagggca agtgggcaga
     5701 ggccaagggg gccaaggcgg tggtggacgc ggcaatcgtg gtcctggggg tcagcaagac
     5761 cccgggcgcg cccagcaatt cgccatgatg cgtgaagcta tggatgagct aacgcagagc
     5821 gcggaagcaa gccttggtaa gattcttgac aagacgcaag ccattcgcct caagcaaatt
     5881 cagcttcaac ttcaaggtct aggcgcgatc ttgcgggaag acatgatgga aaagctcggc
     5941 atcgacgaat cccagatcca gatgttggac gaggtgcggt cgaattaccg caccgcccag
     6001 cgcgagaacg gtcaagcacg ccgggacgtg atgaaagcgg tttttgcgaa ggctatcccc
     6061 aatcagaata atggccaaaa tgccgacaac gcggcgaatg gcggtaacgc cgggaacaat
     6121 ggaaacggca acacagccaa tggcaatgga ggaaatggta atggacgcaa cggcgggcgg
     6181 ggcaatcgcg gtcgcccgga ccccgaggcc atgaagaagg cgatggaaga cccccaaatc
     6241 caggctcaga tggagcagat gaacacccag gaccaaaagc tcgagaacca gttctccctg
     6301 gccatcgcca aagtcctgac cccccggcag cgggcgcttt ataaaaagat gctcggcgcc
     6361 ccgtttgata aatcgaaaat ggggaccgga ggaccatggg gcggacgggg cggcaatggt
     6421 ccgggaggca atccagcaac cgccaaggct ggggcgactg ccggcaagac ggcgactgcc
     6481 accacgaacg caagcgcgga tgacgacgcg gtcgaatcat cgacgaccgc gaagcctgcg
     6541 accacgaccc ctgcgcccgc aaagaccaaa gcggcgacga ccacacctcg aaagaagagc
     6601 ctacgtgaac tccgcgggtc gtcgtccgat agcaacgacc aataagaccc aacctgaaat
     6661 cacttaaggc ccgttataga tgcgacgcgg cgattgggcc cattatgtcg gaacgtggca
     6721 aagttcaact ttaacatctt cgcaggtgac aagtgcgggg tggctggggt cgagttggcg
     6781 acagccaacg agcccccggc gcggaagcct cagatctggg ggtgtcgccc ttcggccgac
     6841 gaccgcagcc atcctcggag gtctaacctg cttttgaaac ttgccaattt ttttgccata
     6901 ttcaaaccgg attttttcgg gtaaccgcac gaacgacgaa cgggacttga caggcgaggt
     6961 tcgcgagcat tcgcttcgaa cgaaccgatc acggacggcc tccgcgaacg aaccggcaat
     7021 atccgttggt gcacgtcccg acttggacac gcgccggtga acttgcggct gtcccgaagc
     7081 ctggaagccc gtgccgcgac gaaggtccag catcgctcaa aatcggcccc agtcgctttt
     7141 gtcatgacct tcaacgctga ttttccccgt atcgaggggg acggtgccgg tcaaattgga
     7201 aggttgtcgg tgggcaggac gtccatccgc cccggtcatg gacaggtttt cgggcttgcc
     7261 ctcactcggc ctgaggttcg cgagcatgta ggcggcgttc gatgtccacg gcacggggct
     7321 cgatataaag tgatttccta ggagatggcg acggcgtcga tcaaggcggt gttgtcgccg
     7381 ccggctgagt ccaatccctg gaaggtgatc gtgtgcgacc cggccgccac cgtgaacgcg
     7441 gcggtgctgt aggcctggta cgacgtgccg gagggggtga agggcgacac gacggtgccg
     7501 tcgaccagca cctcgaaatc ctgggccggc ccgccgtagt tgccgcgctg ggcggcgtcg
     7561 aaggtgataa agtacgagcc gccggcccag ccggcgacgc tctggctgaa cgacccggtc
     7621 atctgaagga cgccgacctg agccccctcg ggggcggggg gattgccggc ggtaaagccg
     7681 ctgccgttgg ccgccacccc ggaaaaaccg gcgaacgacc acgccgtgcc agtcgggtcg
     7741 tacacgaacg agccgtacgc cccggca
//
LOCUS       DATAQL010001289         6331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2424527, whole genome shotgun sequence.
ACCESSION   DATAQL010001289 DATAQL010000000
VERSION     DATAQL010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424527"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VHS97_22210"
     CDS             <1..535
                     /locus_tag="VHS97_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450984.1"
                     /translation="TGYNVYNLDPFVWFVHQQLNLSGYGFSFDDDVSDVGAGGASTLT
                     ITYASGPAANPVASQWFPSTPWGAVTAMATISAPEPSTGDYPGKSILTIQPQAAKAFW
                     QVYANDLNNGLVGAYVSTTAKGISIPPKTNVFFNLNSNALQFVLSTGVTESDTPIPVT
                     FTGYLPAQVLKSVKRRR"
     gene            complement(627..1658)
                     /locus_tag="VHS97_22215"
     CDS             complement(627..1658)
                     /locus_tag="VHS97_22215"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3450985.1"
                     /translation="MITPSLAAVLVLALAFVLPCASFAADLKDVPLPPSELLAPGTAV
                     TVAARTSFLEGPAVDASGDVYFSDMIGNRIYRMTSDGALSVFRPDSGRTNGNTFDAQG
                     RLVSCEGAEQGTGGRRRVVRTDMKTGVVEVLTDRFEGKRYNSPNDVVVDANGRVWFTD
                     PYYGEDRASLELDAEAVYRIETDGKVTRVVSQPQIERPNGLAVSPDTKTLYLIDSNSR
                     PGGNRKIWAFDLSADGTPSNQRLVYDFGRGRGGDGMRLDTRGNLWVAAGITLPRTAAE
                     TADVPPGVYVITPAGKILGRIPIPEDVITNLAFGGPDRKTLYVVAGKAIFKIPVAVQG
                     YALYPPLRP"
     gene            complement(2012..2878)
                     /locus_tag="VHS97_22220"
     CDS             complement(2012..2878)
                     /locus_tag="VHS97_22220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3450986.1"
                     /translation="MQKVIALKTAAEKAQKIAQAATFRFAGKVLSKDGTVIAFDRLGH
                     GWPVILVDGALCYRRMGETGQLAELLAHHFTVYTYDRRGRGDSGDTAPYAVEREVEDI
                     AALLSEAGGEAFIWGISSGAVLAMEATNCLSGIKKLALYEAPFIVDNSRRTTEDDWSR
                     IGEAVVLDRRSDAIKLFLKSVGVPDIFMAVMRLTPVWSKLKAVAHTLPYDGAIVRDNQ
                     KGKPLPATRWRSITVPALVLDGGKSPAWIRHANRSLATVLSNAQYCTLEGQTHMLKPK
                     VHAPILVEFFKN"
     gene            complement(2949..3422)
                     /locus_tag="VHS97_22225"
     CDS             complement(2949..3422)
                     /locus_tag="VHS97_22225"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3450987.1"
                     /translation="MKNIGAAEITTPTDHEIVITRVFDATRAVVFEAWTKAEHVAHWW
                     DPSGVPLAVSEIDLRPGGAFRFVHRAPEGAGHSFTRTYREIVPPARLVFATPAPTGGE
                     SVGILVFEERDGKTVLTMTIACASKADRDALLQMRIDVGTAQTLENLAAYLPKLA"
     gene            complement(3435..3800)
                     /locus_tag="VHS97_22230"
     CDS             complement(3435..3800)
                     /locus_tag="VHS97_22230"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3450988.1"
                     /translation="MEIHAREERLNLAFAALADPTRRAILVRLTRGDADVSELMKPFA
                     LSQPTISKHLNALERAGLVVCTRDAQRRPRTLVAVPLKDIADWLEPFRRFWEERFASL
                     DDYLKDMQKEEKTRGSTKK"
     gene            4088..4873
                     /locus_tag="VHS97_22235"
     CDS             4088..4873
                     /locus_tag="VHS97_22235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3450989.1"
                     /translation="MNREQGRVVAFFGVLVILLWGSCLGFTLWAMKPSESRKLESYGQ
                     LGDVFGSVNALFTGLALVGLVYTVFLQHKQTDALEQQLLHQKEESSRQARELFLTVRL
                     NTLVASIQVQSAQHSALCSDPNMPRGKLAEASMEPLNRAGAALEIMALEASQGFDGGA
                     WTPSVEKEAIRQYIVNLCQEFVASCEKHKANNDPHMASLQTQMARQQFTLLAEMYRAR
                     YPEIFAQCTSILAEFANDSDPTPAINWCRVAETFFQRGQLPWI"
     gene            complement(5063..5917)
                     /locus_tag="VHS97_22240"
     CDS             complement(5063..5917)
                     /locus_tag="VHS97_22240"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3450990.1"
                     /translation="MEIGRAAVTALLMTAAVQGGDVTIEKNISYLPPGRVETLDLYLP
                     PADQKTTAKRPGIVIIHGGGWTGGDKGAKREINIGTTLASHGYVCVSINYALAAEGRP
                     TWPGNLKDCKRAVRWLRKNAEKYGIDPDHIGAIGGSAGGHLTAMLAVTGPEDGFEPDE
                     DQGFSSRVQAAVPMYPGAAAGLNRDHVMFPGKQTEMPEVYRAAAPINHVTRDDAPMLI
                     LHGTADTTTPLSGSQRFADKLEEVGVEHQLVIVEGAPHSFDLQPAQRDLREPVVGFFD
                     KHLRGGKP"
     gene            complement(6054..>6331)
                     /locus_tag="VHS97_22245"
     CDS             complement(6054..>6331)
                     /locus_tag="VHS97_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX3450991.1"
                     /translation="PMTRTCPGGTPAVWRSENGGGSWKRLARGMARKESYFTVLRDAM
                     DVDDLLSPALYFGTTTGQLWIGREGGEEWSCLFDSLPPIHCVKVAVV"
BASE COUNT         1190 a         1915 c         1877 g         1349 t
ORIGIN      
        1 cactggttac aatgtatata acctcgatcc atttgtctgg ttcgtacatc aacagctaaa
       61 tctgtcaggt tatggctttt cgttcgacga tgatgtgtcc gatgtcggtg cgggcggcgc
      121 aagcacgctt accattacct acgcctccgg accggctgca aaccccgtcg cgtctcaatg
      181 gtttccgagt accccgtggg gcgccgtgac cgccatggca acgatttctg cacctgaacc
      241 gtcgactggg gattatccag gcaagtcgat actcacgatc caaccgcagg ctgcaaaagc
      301 tttctggcag gtctacgcga acgacttgaa caatggtctg gtgggagcgt acgtctcgac
      361 cacggcgaag gggatcagca ttccgccgaa gacaaatgtc ttcttcaatc tcaattcgaa
      421 cgcactgcag ttcgtactca gtacaggcgt gacggagagt gacacaccca tcccagtcac
      481 gtttactggt tacctgccgg ctcaggtgct taaatcagtc aagcgtcggc gttagacact
      541 ctcgactctc agctcagctc ggagccgctc gacgttgaac attccctgtt tgagggtgtc
      601 gaatacagaa tccgtgcgcg cacccctcac ggccgcagcg gcggatagag cgcataccct
      661 tggacggcga cgggtatctt gaagatcgcc ttgcccgcga ccacgtacag ggtcttgcga
      721 tctgggccgc cgaaggcgag gttcgtgatc acgtcttcgg ggatgggaat gcgccccagg
      781 atcttgccgg cgggtgtgat cacgtaaacg cccgggggga cgtcggcggt ctcggccgcg
      841 gtgcgcggga gggtaatgcc ggcggcgacc cagagatttc cccgggtgtc gagccgcatg
      901 ccgtcgccgc ctcggccgcg gccgaagtcg tacacgagcc gctggttcga gggggtgccg
      961 tcggccgaga ggtcgaacgc ccagatctta cggttgccac cggggcggga attcgagtcg
     1021 atcaggtaga gtgtcttggt gtcgggcgac acagctaagc cgttgggacg ctcgatctgg
     1081 ggctgcgaga ccacgcgcgt gaccttgccg tcggtttcga tccggtacac ggcctcggcg
     1141 tcgagctcca ggctcgcgcg gtcctcaccg tagtacgggt cggtgaacca gacccggccg
     1201 ttcgcgtcga cgacgacgtc gttcgggctg ttgtagcgct tgccttcgaa tcggtcggtg
     1261 agcacctcga cgacgcccgt cttcatgtcg gtgcggacga cgcggcggcg gccgccggtt
     1321 ccttgctcgg ccccctcgca gctgacgagc cggccctggg cgtcgaacgt gttgccgttg
     1381 gtgcggccgc tatcggggcg gaagacggag agggccccgt cggacgtcat cctgtagatg
     1441 cggttgccaa tcatgtcgct gaaatagacg tcgcctgatg cgtcgacggc cggtccttca
     1501 agaaagcttg tccgcgcggc gaccgtgact gcggtcccag gcgcgagcag ctcggacggc
     1561 ggcaatggga cgtccttgag atcggccgca aacgaggcgc acggtaggac gaatgcgagc
     1621 gcgagcacca agactgcggc gaggctggga gtgatcatcg atttcgggct ccggttgcgc
     1681 ggggaggaca caaagcgatc gggacaaccc aagtaaggtg cgcgaaacgc gacgggtttt
     1741 caagcaggcg gaaaggcatt gcaacgccag tcgtttcccg ctgggctaaa gccgtacaca
     1801 accctgaccc ttttattgcg aagcgattcg gatctcccgg caatgcctgg gctcagctga
     1861 ttccactttc gcgcggccga atgttcgagc tcggaccgcc cgaagtaccg gggccgatat
     1921 gccgctgaag cgattgtcgt tccctgagga agagggggac cgatcttggg atgcaatgcc
     1981 ttgtccattc cactcttctc cgcccgtcta cctaattctt gaaaaactcc accaaaattg
     2041 gcgcgtgcac cttcggtttc agcatgtgcg tctgcccttc gagcgtacaa tactgcgcgt
     2101 tcgacaatac ggttgcgagt gacctgttcg cgtgccgaat ccatgcgggg ctttttccac
     2161 cgtccaggac gagtgccggc accgtgatgg acctccaacg ggtagccggc aacggcttgc
     2221 ccttctggtt gtctcgtaca atcgcgccgt cgtagggtag cgtgtgcgca acggctttaa
     2281 gcttcgacca aacgggtgtt agtctcatta ctgccatgaa gatgtcagga actccgaccg
     2341 acttcaggaa gagcttgatt gcatcgcttc gccgatctaa gacaaccgcc tcgccgatcc
     2401 gggaccaatc gtcctcagtt gttcgacggc tgttatccac tatgaatggc gcctcgtaca
     2461 atgccagctt cttaatgcca ctgagacaat tcgtcgcttc cattgcgagc actgcgccgg
     2521 aggagatgcc ccaaatgaac gcttcgccac cagcttcact cagcaacgcg gcgatgtctt
     2581 cgacctcacg ctccacagca taaggtgccg tatcaccact gtcgcctcga cctcgccggt
     2641 cgtaggtgta gacggtaaag tgatgtgcca ggagttcagc cagttgtcca gtctcgccca
     2701 tccttcgata gcagagtgcg ccgtccacca gaatcaccgg ccaaccatga ccgagtctgt
     2761 cgaatgcgat cactgtgcca tccttcgaga ggacctttcc ggcaaaccgg aaagtcgccg
     2821 cctgcgcaat cttttgggct ttctctgcgg cagtcttcag agcaattacc ttctgcatag
     2881 atcctcccac tttttggctt cgatgctttt ctacaaactt cgcgctcttg tttaacctgc
     2941 cgttcggctt atgcgagctt gggtaggtat gccgcaaggt tttccagagt ttgagccgtg
     3001 ccgacatcaa tgcgcatctg gagcaaagcg tcgcgatccg ccttcgacgc acacgcgatc
     3061 gtcatcgtga gcactgtctt gccgtcgcgc tcctcgaata cgagtatgcc gaccgattcg
     3121 cctccagtcg gtgcgggcgt cgcgaaaaca aggcgcgccg gcggcacgat ctcgcgatat
     3181 gttcgcgtga aggaatgtcc cgcaccttcc ggcgcgcgat gaacgaaccg aaacgcaccg
     3241 cccggccgga ggtcgatttc ggagacggct agtggcacgc cgctcgggtc ccaccaatgt
     3301 gcgacatgtt ccgctttagt ccaggcttcg aaaactaccg cgcgcgttgc atcgaagacg
     3361 cgcgttatca cgatctcgtg atcggttggt gttgtgattt ccgctgctcc gatgttcttc
     3421 attggtgctc tcccttattt cttcgtgctt ccacgtgttt tctcctcttt ttgcatatcc
     3481 ttcaggtaat cgtccagact cgcaaatctc tcctcccaga agcgacgaaa cggttcaagc
     3541 cagtccgcga tgtccttgag tggaacggcg acgagcgtac gcgggcgccg ctgtgcatcg
     3601 cgtgtacaca caacgaggcc tgcgcgttcg agcgcgttga ggtgtttaga tattgtcggc
     3661 tgactcagtg cgaacggctt catgagctca gaaacatctg cgtctcctcg cgtgagacgg
     3721 acgaggatcg cgcgtctcgt tgggtccgcg agagctgcaa aggccaaatt gaggcgttct
     3781 tctcgtgcat gtatttccat tgagctatat tgctacttgg caatataaaa gtcaagcaca
     3841 aaagttccaa acttagccgc acttcaggcc ttgcaatgcc agccttgtcc ctggcggctg
     3901 acgctataca caaccctacc cgtttattgc gagatgatta tactttgact tatcagcgga
     3961 tagaccgtcc ttagcgaccg tttcctgtcc ctcgggcgat agattcgcta ttgtcgacca
     4021 tgctggcgtc tgtctcgatg ttcaagaccg gcgcgcgata cctgtcgaag cgcaggaggt
     4081 tgaaaagatg aaccgagagc agggccgcgt tgttgctttc tttggagtat tagtgatcct
     4141 tctgtggggg tcatgtcttg gattcacgct gtgggcaatg aagccgagcg aatcgcgcaa
     4201 actcgaatcc tacggtcaat tgggcgacgt gtttgggtcg gtaaacgctc tttttaccgg
     4261 attggcactt gtagggctgg tctacacagt ctttctccaa cacaagcaga ccgacgccct
     4321 ggagcaacag ctcttgcacc agaaggaaga atcaagcagg caagcgagag agctattctt
     4381 gacagttcga ctcaatacct tagtggcgtc gatacaggtg caatctgctc agcattcggc
     4441 gctgtgttcc gacccgaaca tgcctcgagg aaagttggcc gaagcgtcga tggagccgct
     4501 caacagggcc ggtgcagccc tcgaaatcat ggccttagag gcgagccaag ggttcgatgg
     4561 cggagcatgg acgccatcgg tagaaaaaga agctatacgt caatacatag tcaatttgtg
     4621 tcaggaattc gtggcctcat gcgaaaaaca taaggcgaac aacgatcccc acatggcttc
     4681 gctgcaaacg caaatggctc ggcaacaatt cacgctgctg gctgagatgt atcgtgcgag
     4741 atacccagaa atatttgccc agtgtacgtc catcttagct gagttcgcaa atgacagcga
     4801 tccgacacca gctattaact ggtgccgggt tgccgaaacc ttttttcagc ggggccaatt
     4861 gccctggatc tgatgtacca gggccggtat gtggcttaag cgtcagtagt tccttgagga
     4921 agacagggac cgacctgggg ctgcggacca tgtcgttgaa gtctttgaag tcctgtcctt
     4981 tgcagtccct ttccgctttg gatcgctcga gcccggtacg ctctgcatgt cctttcaacc
     5041 ccagggaacc gtcgcgcccc tcctacggct ttccgcccct caggtgcttg tcgaagaacc
     5101 cgaccacggg ctccctcaga tcgcgttgcg cgggctgcag gtcgaagcta tggggcgccc
     5161 cctcgacgat cacgagctgg tgctcgacgc cgacctcctc gagcttgtcg gcgaagcgct
     5221 gggatcccga gagcggcgtg gtggtgtcgg ccgtaccgtg cagtatgagc atcggcgcgt
     5281 cgtccctggt gacatgatta atcggcgcgg cggcgcggta gacctcgggc atctcggttt
     5341 gcttgccggg gaacatgacg tggtcgcggt tcaggcccgc ggcggcgccg ggatacatcg
     5401 ggacggccgc ctgcactcgg ctggagaagc cttggtcctc gtccggctcg aacccgtcct
     5461 ccggaccggt caccgcgagc atggccgtca ggtgcccgcc ggccgacccg ccgatggctc
     5521 cgatgtggtc gggatcgatt ccgtacttct cggcattctt gcggagccag cggacggccc
     5581 gcttgcagtc cttgaggttg cccggccacg tcgggcggcc ctccgcggcc agggcgtaat
     5641 tgatgctgac gcacacatag ccatgcgagg cgagtgtcgt gccgatgttg atctcgcgct
     5701 tcgccccctt gtcgccccca gtccagccgc cgccgtggat aatcacgatg cccggccgct
     5761 tcgcggtggt cttctggtcc gcgggcggga ggtagaggtc gagcgtctcc actcggccgg
     5821 gcgggagata gctgatgttc ttctcgatcg tgacgtcccc gccttgcacc gccgccgtca
     5881 tgagcaacgc ggtgaccgcc gccctgccga tttccatcgc tgtgcctccc gccgaggaac
     5941 ccgccccgat cgaacgccga cctagtccgc agagcgatca tccgcgatcg gccagacgaa
     6001 ggcaacacac tgctcaccgc aacctcatcg cgccggccgc gctgttgtcg ggctcacacc
     6061 accgcgacct tcacgcaatg gatcggcggc aatgagtcga agaggcaact ccattcctcg
     6121 ccgccttcac gaccgatcca gagttggccg gtcgtggtgc cgaaatagag cgcgggcgag
     6181 aggaggtcgt cgacatccat cgcgtcccgg aggaccgtga agtagctctc tttccttgcc
     6241 attccgcgcg cgagccgctt ccacgagccg ccgccgttct cgctgcgcca gaccgcgggg
     6301 gtgccgcccg ggcaagtgcg cgtcatcggc t
//
LOCUS       DATAQL010001290         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3184642, whole genome shotgun sequence.
ACCESSION   DATAQL010001290 DATAQL010000000
VERSION     DATAQL010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184642"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VHS97_22250"
     CDS             <1..835
                     /locus_tag="VHS97_22250"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX3450992.1"
                     /translation="YARFEAKKLVADIMDADIRQRTEKVLAKAKNVCVVEDVFPKLAD
                     NSGETPTIKDDPPFIYHHMERGEDEFYERIEEVLFRYRESLPDDRRVLLDRYAVKDKA
                     IKVVGVGSVGTVCSIALLMAGEKDPLFLQVKEARPSVLEAFAGPSIYPNHGQRVVKGH
                     RLMQSASDIFLGWTEDGTGRHFYVRQLRDMKLKFRIEEFKSAKMVIFAEWCGGTLARA
                     HARSGEPALISGYLGKADTFDRAIGDFAVAYADQVEHDYENFSKAVRDGKLEAVTDDR
                     V"
     gene            complement(1138..3117)
                     /locus_tag="VHS97_22255"
     CDS             complement(1138..3117)
                     /locus_tag="VHS97_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX3450993.1"
                     /translation="MDFFQNQEVARQKTGTLVFFFVLAVVLIVLSVYCAVATVLEYAE
                     PSSPDGTVSAVHSLWNPELFGAVALGTSALIAGGSLYKVAALSGGGHTVAELLGGRLL
                     HPDQANLDERRVLNVVEEMAIASGLPVPPVYLLENEPAINAFAAGHTTGDAIIAVTSG
                     TVKRLTRDELQGVIGHEFSHILNGDMRLNIRLMGVLFGILVIGLTGWIIFRSSTGGGY
                     VRMGARDDDDRKGFNPLPLIGLALYVIGYVGVFFGNLIKAAVSRQREFLADASAVQFT
                     RNPEGLAGALKKIAALDVGSQIGDPHAEEASHMFFGEAVRGFNELFGLLSTHPPLVER
                     IRRIDPSFNGDFSQVSVEMIPQEAPQAQRARSNGWPRPGVLGLTAARAVAGVGTLDQE
                     HIDYAADLKESIPRSLTESLRDPLGAQAVVFALLLDPGEAVRRLQLAWLDAHALPAAT
                     RETKKLAALAHGLAPEARLPLVEMAAPALCQMTPAQFHDFIRCVEALVRADQKMTPFE
                     YALQRLLIRHVVAHFVRTRPPIVKYTTIPPLVTPISVVLSALAYAGESTSEGAARAFA
                     AGVHALGRPGVQLGLAPPGTVGLSEIDAALEVLAMAAPQLKKQILQACAACICVDGTV
                     TIEEGELLRAVSDSLGCPMPPLIASGPDPQRVQND"
     gene            complement(3282..>3797)
                     /locus_tag="VHS97_22260"
     CDS             complement(3282..>3797)
                     /locus_tag="VHS97_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX3450994.1"
                     /translation="LVALRNRYKNAFSQIDVQLKRRYDLIPNLIEVAKGYMGHERATL
                     EAVIQARNQAVTAQQRAAANPGDPAAMSGLSAAEGQVSGVLGRLFALAESYPDLKANT
                     NMLALQEELTSTENKVAFARQAFNDAVMTYNTQREVFPASVIANLAGFAPAQLFEIES
                     PKEREAPKVSF"
BASE COUNT          726 a         1161 c         1210 g          700 t
ORIGIN      
        1 gtacgcgcgg tttgaagcca aaaagctcgt ggcggacatc atggacgccg acatccgcca
       61 gcgaaccgag aaggtcctcg cgaaagcgaa gaacgtatgc gtcgttgaag acgttttccc
      121 caagctagcc gacaactcgg gcgagacccc aacgatcaag gacgacccgc cctttatcta
      181 ccatcacatg gagcgcggcg aggacgaatt ttacgagcgg atcgaggagg tcttgtttcg
      241 ctaccgcgaa tcgctgcccg acgatcggcg agtcctcctg gaccgatatg ccgtcaaaga
      301 caaggccatc aaggtggtgg gtgtcggcag tgtcggtacc gtttgcagca tcgcgctctt
      361 gatggcgggt gagaaggacc cgctgttcct ccaggtcaag gaagctcgcc cctcggtgtt
      421 ggaggcgttt gcgggcccga gtatttatcc caatcacggg caacgggtcg tgaaggggca
      481 ccggctcatg caatcggcca gcgacatctt cctgggctgg accgaggacg gcaccggccg
      541 gcatttctac gtccgtcagt tgcgggacat gaagctcaag ttccgcatcg aggagttcaa
      601 atcggccaag atggtcatct tcgccgagtg gtgcggtggc acgctggcgc gcgcccacgc
      661 gcgttccggc gaaccggctt tgatcagcgg ctacctcggc aaggctgaca cgttcgatcg
      721 agcgattggg gactttgcgg tcgcttatgc cgatcaggtt gagcacgatt acgagaactt
      781 ttcgaaggcg gtacgcgacg ggaaattaga agccgtgacc gatgaccgcg tctgagtcgt
      841 gtcgggtgcg gtctgagatg tgagagaagt ggcggcgacg acaacgctca gccctgagcg
      901 ttacccattt tgtgtggcct tgcactggtt tgtcgtcgcc gccggagtct tctgctgtct
      961 caaccgtaaa atctcggcga ccgcgacacg cacggcccac aaaagtcgta tatttttccc
     1021 caataattcc gatcgtgccg acaccatgac aaaaaaggga cctagtgcat cgcctgggag
     1081 ccaaacgagc ccggacggcg tctgggccgt tcgagcgggg tatcagtttt ggtaggttta
     1141 gtcattctga acccgctgcg gatcgggacc gctggcgatc aaaggtggca tagggcagcc
     1201 cagcgaatcc gagacggctc gcagcaattc accctcttcg atggtcacgg tgccgtcgac
     1261 acagatgcag gctgcgcaag cctggagaat ttgcttcttg agctgtggtg ccgccatggc
     1321 gagcacttcg agcgcggcgt cgatctcact gagcccgacg gttcccggcg gtgccagtcc
     1381 gagctggacg cccggccgtc cgagagcgtg cacgcccgcc gcgaaggcgc gagcggcccc
     1441 ttcggacgtg ctttcgccgg cgtatgccag agccgagagg acaacggaaa tcggcgtgac
     1501 cagaggaggt atcgtcgtgt acttcacaat cggcggccgg gtgcgcacaa aatgcgcgac
     1561 cacgtgccgg atcaagagcc gctggagggc gtactcaaat ggtgtcatct tctggtctgc
     1621 tcgcaccagg gcctcgacgc agcggatgaa gtcgtgaaac tgggcgggcg tcatctgaca
     1681 cagggcaggg gcggccatct cgaccaaggg gaggcgagcc tccggagcca ggccatgggc
     1741 tagggcggcg agctttttgg tctcacgcgt cgcggcgggc agggcgtgcg cgtccagcca
     1801 ggcaagctgg agtcgtcgaa cggcttcgcc gggatcgagc aagagggcga ataccacagc
     1861 ctgggcgccc agagggtcgc gtagtgattc cgtaagtgac cgcgggatcg attccttgag
     1921 gtcggcggca tagtcgatgt gttcttgatc gagggtcccc acacccgcga cggcccgcgc
     1981 tgccgtgagg ccgaggaccc caggccgtgg ccatccgttc gaccgcgctc tctgagcctg
     2041 cggggcttct tgcgggatca tttccacgct gacttgagaa aagtcgccgt tgaacgaagg
     2101 atcaattcgc cggatccgct ccaccagggg agggtgcgtc gacagcagtc cgaacagttc
     2161 attgaagcct cgcacggcct cgccgaaaaa catgtggctc gcctcctctg cgtgcggatc
     2221 gccgatttgc gagcccacgt cgagtgctgc gatcttcttg agagcgcccg caagtccctc
     2281 tggattgcgg gtgaactgca cggccgatgc gtcggccaga aactcgcgct ggcgcgacac
     2341 ggcggccttg atcaggttgc cgaagaaaac tccgacgtag ccgatcacgt agagggccag
     2401 tccgatcaac gggagcgggt tgaagccctt gcggtcatcg tcgtcgcggg ctcccatccg
     2461 cacgtaaccg ccgcccgtcg agctgcggaa gatgatccag ccggtgagtc caatgaccag
     2521 gatgccgaaa agcacgccca tcagccggat attgagccgc atatcgccgt tcaagatgtg
     2581 gctgaattcg tggccgatca cgccttgcaa ctcgtcgcgc gtgagccgct tcaccgtgcc
     2641 cgaagtgacc gcgatgatgg catcgcccgt agtgtggccg gcggcgaacg cgttgatcgc
     2701 cggctcgttc tcgagcagat aaaccggggg taccggcaag cccgaggcga tggccatctc
     2761 ttcgaccaca ttcaagaccc gccgctcgtc gaggttcgcc tggtcggggt gaagcagccg
     2821 gcctcccagc aactcggcca ccgtatgccc gcctccggag agcgcggcca ccttgtacag
     2881 actccctcca gcgatgagcg cgcttgttcc cagggcaacc gctccgaaga gttccgggtt
     2941 ccacaaactg tggacggcac tcaccgtccc gtccggactg gacggctcgg cgtactcgag
     3001 tacggtcgcg acggcgcagt agaccgagag tacgatcagg acgaccgcca gcacgaagaa
     3061 aaagacgagc gtgcccgtct tctggcgcgc gacctcttgg ttttgaaaaa aatccatgcg
     3121 tgatcagcct cagtcacgcc gcttcgcggc acgggcattt tgaccgtcgc aaccattttc
     3181 acgggctgga agcccgtgcc acactgtcgg cacgatctga acgtttggcg ttcgcgcgtc
     3241 gcaaccatcc tcacgggctg gatgcccgtg ccacgatggg atcagaacga aaccttgggc
     3301 gcctcgcgct ccttgggaga ttcgatctcg aagagttgcg ccggcgcgaa tccggcgaga
     3361 ttcgcaatca cggacgccgg aaaaacctcg cgctgggtgt tgtaggtcat caccgcgtcg
     3421 ttaaacgctt gccgagcaaa tgcgaccttg ttctcggtag acgtcagctc ttcctgaagc
     3481 gcgagcatat tggtgttggc cttcaggtcg ggatagctct cggccagagc gaacaggcgg
     3541 ccgagcaccc ctgagacctg cccctcggcg gcactcaacc cgctcatcgc agccggatca
     3601 cccgggttcg cagccgccct ctgctgggcg gtcacagcct ggtttcgagc ctgtatcacg
     3661 gcctcgagcg tcgcgcgctc gtggcccata taccccttgg ccacctcgat caagttggga
     3721 atcaggtcgt agcgccgctt caactggacg tcgatctggc tgaacgcgtt cttgtagcgg
     3781 ttgcggagcg cgaccag
//
LOCUS       DATAQL010001291         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2268286, whole genome shotgun sequence.
ACCESSION   DATAQL010001291 DATAQL010000000
VERSION     DATAQL010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268286"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VHS97_22265"
     CDS             <1..219
                     /locus_tag="VHS97_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX3450995.1"
                     /translation="MFFACGTLNQELTRPVNDLDFEVALHIAFDSKEAHDAYQDAPSH
                     HRFINENKANWRQVRVFDADVESHASSA"
     gene            513..1868
                     /locus_tag="VHS97_22270"
     CDS             513..1868
                     /locus_tag="VHS97_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3450996.1"
                     /translation="MTSTWSVLVIDDDPGIRQSLRLCMETDGARVFGVGTATGALEAI
                     ERGRFDVVFLDLWLQSESGLALLPELLRRQPGVCIIVITAFATFETAVEAMKLGAVDY
                     LPKPFAPEQVRTAARRVVTANVLKRRLNELEDRLDETQAEGRFEARSPVFAAFLQTAT
                     RAAASEAVVLLRGESGTGKTVCARWIRSHSRRADGPFVTVNCPMLSTDLMSSILFGHR
                     RGAFTGAVADSIGKVEEAEGGTLFLDEIGDLGMDAQARLLRFLNDRTYERLGEAKERK
                     ADVRLLAATNRSLEEEVQAGQFREDLLFRLNVISLTLPPLRDRREDVLPMALHYLGSL
                     ERLQGRPHLSFSPGCEQTIATYSWPGNLRQLHNAVERAVILAPSTVLEVADLGLSAVT
                     HSDPDRTAVTLGADISLEEIEREHLARVVARAPSFEAAARILGIDVTTLQRKRKRYGM
                     S"
     gene            1996..>3859
                     /locus_tag="VHS97_22275"
     CDS             1996..>3859
                     /locus_tag="VHS97_22275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3450997.1"
                     /translation="MRIRRLQTRFLLAGCLLAMITLGSGLWSAWTFAQLSRVADRVLH
                     DSQTTIDLAAELAGSLEREDDALLLALSGNGLRAPRDLADERRRGDDCFGRLRSRLSI
                     DRADVDEIISELRREMDGYRASGSVLVQQSERSDALAHYHERVNPRLRRAVQVCSQIR
                     EREFAAMRLASVQARDEARAATRIVMTASVAALLLAMVVAGWLVRSVALPIRRLSSSV
                     EALRLGDFEQRVALTSEDELGRLAAGFNRMAETLAEYRRSSVGELIAAKTTLESTLNA
                     LPDAVFVIAPDGTFAALNPPARAILQAKRVGAGRRVHDLPLAQEDHEAIEAALAGRTV
                     LPAPADFSRVIQARLNGQPRKFLVSAAPIPEFAPRQTGAVVVLDDVTEFARLDELRSE
                     LVAVASHELKTPLTTMRMNLMLLGEATESFTFRQHEMLEAALLGCEELGSTIDELLDV
                     ARIEAGELRLDLAPIDLNTILDAALRLLQARIDDADLRLEVRREARPAIVHGDPTRLR
                     NVMTNLLANALKYSPRAGRVLIEIVSRHAADPLGPATLQFAVTDEGPGVPEPFRERIF
                     EKFFRVEHHVESAPEGVRGTGIGLYLCREVIKAHGGSIWCEPAQGGVGTVIAFRL"
BASE COUNT          658 a         1272 c         1229 g          700 t
ORIGIN      
        1 atgttcttcg cgtgcggcac cctcaatcaa gaactgaccc gtcctgtcaa cgatctcgat
       61 tttgaagtcg ccctccatat cgctttcgat tccaaggaag cacatgacgc ctatcaagat
      121 gccccgtcgc atcaccgttt catcaacgaa aacaaggcga actggcggca ggtgagagtc
      181 tttgacgccg acgtcgagtc acacgcgagc tcggcttagg gacattgcag ttgaatgagc
      241 agcgacttac gggaccgtgg taatcacact catcgcgctc ttgatctcat tgctgctgca
      301 cgcggtgcag gcggcacgag aggccgcctg acgcacgccg tgcaacgctc ctcatccgct
      361 ggctcacgcc ggtttactaa gcgcgactta tcggggtaga ttggaaagct ggccggcagg
      421 ttgggcgtcg gcgacttggc tggaatatta aaacgctccg cggagacgcg agcggagcag
      481 agtcactcga attcctggcg gcgacccagt ccatgacctc cacctggtcc gtactcgtca
      541 tagacgacga tccgggaatc cgccaatccc tccggctctg catggagacc gatggtgcgc
      601 gggtcttcgg ggtcggcacc gcaacgggcg cgctcgaggc gatcgagcgc ggccgcttcg
      661 acgtagtctt cctcgatctg tggctccagt cggaatctgg gctggcgctg ctaccggaat
      721 tgctccggcg ccagccgggt gtgtgtatca tcgtcatcac ggccttcgcc acgttcgaga
      781 cggctgtcga agcgatgaaa ctgggggccg tggactacct gcccaaaccg tttgcgcccg
      841 agcaggtgcg gacggcggcc cgccgggtcg tgactgcaaa cgtcctcaag cgccggctca
      901 atgagctgga agaccggctc gacgagacgc aggctgaggg ccgcttcgag gccaggagcc
      961 cggtctttgc cgcgttcctc cagacggcaa cgcgggcggc cgcctcggag gcggtcgttt
     1021 tgctgcgcgg agagagcggc accggtaaga ccgtttgcgc ccgctggata cgctctcaca
     1081 gccgccgtgc cgacgggccg ttcgtcactg tcaactgccc tatgctctcc accgatctga
     1141 tgagcagcat cctcttcggc catcgccggg gcgccttcac cggcgccgtc gccgattcca
     1201 ttggcaaggt cgaggaagcc gaagggggca ccctcttcct cgacgagatc ggcgacctcg
     1261 gcatggacgc ccaggcccgc ttgctccgat tcttgaacga ccgcacttac gagcgcctgg
     1321 gtgaggccaa ggagcgcaag gcagacgtgc ggctgctcgc agccaccaat cgctccctcg
     1381 aagaagaggt gcaggctggt cagttccgcg aggatctcct gttccgcctg aacgtgatca
     1441 gcctgaccct ccccccgctt cgcgatcgcc gcgaggacgt cttgccgatg gccctccact
     1501 acctcggcag cctggaacgc cttcaggggc ggccgcatct gtcgttttcc cccggatgcg
     1561 aacaaaccat cgcgacttat tcgtggccgg gtaatctgag gcagttgcat aacgccgtcg
     1621 aacgtgccgt catcctcgcc ccctcgactg ttctcgaggt ggccgacctc ggcctgtctg
     1681 ccgtcaccca ttccgatccg gaccggactg cggttaccct gggcgccgac atatcgctcg
     1741 aggagatcga gcgcgagcac ctggcacgcg tggtggcccg tgcaccttcg ttcgaggccg
     1801 cggcgcggat cctgggtatc gacgtgacga ccctgcagcg aaagcgcaag cggtacggaa
     1861 tgtcgtgatc ggccgcgagc caggcggccg aaggcgtggt caatgcgcag cctcagttca
     1921 gagtggcccg gcatggtcga ggcgctcgta caaaacggtt gaactcagcg accatcttgc
     1981 agagaacagt gatcgatgag gatacgacgc ctgcaaaccc gattcctcct ggcaggatgc
     2041 ctgctcgcca tgatcaccct tggcagtggg ctctggagcg cctggacctt tgcgcagctt
     2101 agccgcgttg ccgatcgggt gctgcatgac agccagacga cgatcgacct ggcggccgag
     2161 ctggcaggat cgctcgagcg agaagacgat gctctcctcc tggcgttgag tggcaacggt
     2221 ctgcgggcac ctcgcgacct ggccgacgag cggcgacgcg gcgacgattg ctttggacga
     2281 ctccggtcac ggctttcgat tgaccgtgcc gacgtcgatg aaatcatctc ggagctgcgc
     2341 cgcgagatgg acggctaccg cgcgtctggg tcggttctgg tgcagcagtc cgagcggtcc
     2401 gacgcgctcg cgcattatca cgagcgagtc aatccgcggc tgcgcagggc ggtccaggtg
     2461 tgcagccaga tccgcgagcg cgagttcgcc gcgatgcggc tagccagcgt tcaggcgcgc
     2521 gacgaggcgc gtgccgcgac gcgaatcgtc atgactgcgt ccgtggccgc tcttttactg
     2581 gcgatggtcg tcgcgggctg gctcgtacgg tcggttgcct tgccgatccg caggctgagc
     2641 tcgtcggtgg aggcacttcg tctcggcgac ttcgagcaac gcgtggcgct gacctccgag
     2701 gacgagctcg gccggctcgc ggctggcttt aaccggatgg ccgaaacact cgctgagtac
     2761 cggcggtcca gcgtggggga gctaatcgcg gcgaagacga cgctggaatc gaccctcaat
     2821 gcgctgcccg acgccgtctt cgtcatcgcc cccgacggca ccttcgccgc gctcaacccc
     2881 cccgctcgcg caatattgca ggccaagcgc gtcggcgccg gccgtcgggt tcacgatctg
     2941 cccctggcgc aggaagacca cgaggccata gaggcggcgc tcgctggtcg gaccgtcctc
     3001 ccggcgccgg ccgatttctc tcgcgtgatt caggcacgcc tgaacggtca gccgcgtaag
     3061 ttccttgtat ccgcggcgcc gatccccgag ttcgcaccgc gccaaaccgg ggccgtggtc
     3121 gtgctcgatg acgtaaccga gttcgcccgt ctggacgaac tgcggagcga gctggtcgcg
     3181 gtggcttcgc atgagctcaa gactccgctg acgaccatgc gaatgaacct tatgctcctc
     3241 ggcgaggcga ccgagtcctt cacattccgg cagcatgaga tgttggaagc ggccctgctc
     3301 ggctgcgagg agctcggaag cacgatcgac gaactgctcg atgttgcccg catcgaggcc
     3361 ggggagctcc gccttgacct cgccccgatc gacctgaaca ccatcctcga tgcggcgctc
     3421 cgcttgcttc aagcacgaat cgacgacgcg gacctgcggc tggaggtccg gcgcgaggca
     3481 cggcccgcaa tcgtccatgg cgatccgacc cgacttcgga atgtcatgac aaatctgctc
     3541 gcgaacgccc tcaaatactc gcctcgggcc ggccgtgtcc tcatcgagat cgtatcccga
     3601 cacgctgccg atcctctcgg cccggcgacc ctgcaatttg ctgtgaccga cgaggggcca
     3661 ggggtcccag aaccttttcg ggagcgcatt tttgagaagt tctttcgcgt cgagcaccac
     3721 gttgaaagtg ccccggaggg cgtgcgtggt acgggaatcg ggctctacct ctgccgcgag
     3781 gtcatcaagg cccacggcgg ctccatctgg tgcgagcccg cccaaggcgg agtcggcacg
     3841 gtcatcgcgt tccgactcc
//
LOCUS       DATAQL010001292         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2879925, whole genome shotgun sequence.
ACCESSION   DATAQL010001292 DATAQL010000000
VERSION     DATAQL010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2879925"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VHS97_22280"
     CDS             <1..985
                     /locus_tag="VHS97_22280"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3450998.1"
                     /translation="LDCVRLTGGEAEACGMEAPPVERRLAAILAADVEGYSRLMHGDE
                     EATMAALSASRALVDDLIGRHRGRIANTAGDSVLAEFASVLDAVHCAVEIQEALTRAN
                     YGEPDGRRMLFRIGVNVGDVMFKEGDIFGDGVNVAARLEGLVKGGEICVSRGVHDHLR
                     HRGMAFDDLGEQLVKNIAHPIRAFRLRIGGDQSEQEVPTPGKLAESPDPPSAPPAISE
                     LSADNEVALELALWDSVEDGSPAELESYLERYPEGTFASLARTRLDAAALSPAEPAAP
                     TPEEVAAEALDLAFWNSVKDSNRREELQAYLEQHPNGHFAGLARARLSSSE"
     gene            1215..1670
                     /locus_tag="VHS97_22285"
     CDS             1215..1670
                     /locus_tag="VHS97_22285"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3450999.1"
                     /translation="MEPTNRLVTTPPAPRGSAIPKSVSTPHRVGIHASHIPPTSLGTL
                     AVGVVTVAALYFGREVVVPMALAILLGFALAPLVLLLRRWHLGRVPSVIAVVVLAFLM
                     IVGIGAFVGNQLAHLAGELPGYQTNIAQKIRSLRDTTAKGALSTAPRRC"
     gene            1664..2719
                     /locus_tag="VHS97_22290"
     CDS             1664..2719
                     /locus_tag="VHS97_22290"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3451000.1"
                     /translation="MLSNLNNEITEPEEKEGGSVGNQVGALPHNNQPQDPVPVEIRRH
                     NPSPFLLFQQFAVPLLQPFATAGIVVVFVIFFLLQREDLRDRFIRLAGAGDLRRTTLA
                     LDDAASRLSRYLLTQTAINTSFGVLIGTGLWFIDVPHPMLWGILGMLLRFVPYIGPVI
                     AAALPAIVAFAVDPGWAMLLWAIALFLVVELAIGQVIEPWLYGHSTGLSGIAVVVAAA
                     FWTLLWGPVGLLLSTPLTMCLVVLGRHVEQLQFLEVLLGDRPALAPEEAFYQRMLAGD
                     PDEAAHQAEEFLQNKPLAAYYDEVAIGGLTLAQWDVNRGGAGTRAPGPDQRVGRRGHR
                     QSCRPRPVGCSESGREW"
BASE COUNT          507 a          900 c          911 g          567 t
ORIGIN      
        1 gcttgattgc gtgcggctaa ctggaggtga agcagaggct tgcgggatgg aggcgcctcc
       61 agtcgagaga cgtctcgcgg caattctcgc cgcggatgtc gagggctaca gccggctaat
      121 gcacggcgac gaagaagcaa caatggcggc gttgtccgcc agtcgtgcac tcgtcgacga
      181 cctgattggg cggcatcgag gccggatcgc gaacaccgcg ggagatagcg tgcttgcgga
      241 attcgcgagc gtgcttgacg cggttcattg cgcggtcgag atccaagagg cgcttacacg
      301 tgcgaactat ggtgagccgg acgggcgacg gatgctcttc cggattggcg tcaatgtcgg
      361 cgacgtcatg ttcaaagaag gcgacatctt cggtgatggc gtgaatgtcg ctgcccggct
      421 cgagggactg gtcaagggcg gcgagatctg cgtttcgcgg ggcgtgcacg atcacctgcg
      481 tcatcgcggc atggcctttg atgatctggg cgagcagctg gttaagaaca tcgcgcaccc
      541 gatccgggcg tttcggctcc gtatcggcgg tgatcagtcc gagcaggagg tcccgacccc
      601 cgggaagttg gctgagtcac ctgatccgcc ttccgccccg ccggccatct ccgaactctc
      661 cgccgacaat gaggtcgctc tcgagctcgc gctttgggac agcgtcgagg acggcagccc
      721 tgccgagctc gaatcctacc tcgagcggta tccggaaggt acctttgcct cgctcgcccg
      781 aacacggctt gatgccgccg cgctctcgcc cgcagagcca gcggcgccga cacccgagga
      841 agtcgccgct gaggctctcg acctggcctt ctggaactcc gtcaaagaca gcaatcgacg
      901 tgaggagctt caggcctatc tggaacagca tccaaacggc catttcgccg ggctcgctcg
      961 ggcgcgcctc tcttcctcag agtgacttag cgtgcatcgc cggatcgacg gctgtccgtt
     1021 caagcgccgg ggagataaag cgggagtgca cgcgcgtcgc acgggggtcc tgctgatcag
     1081 cttgccgacc gatcccagcg gcgagcctgt ttttacgatc ggcgcggtgg gggacaagcc
     1141 gacgcgcagc ctgccgtgcg ccagagccac tcggttcgcg gcgccgtcac aaatgtcaag
     1201 caatacaggg ccgaatggaa ccgacaaacc gattagtgac tacgcctccg gcaccgcgag
     1261 ggtccgcgat acccaaatcc gtttccaccc ctcatcgggt ggggatccat gcctcccata
     1321 ttccccctac aagtctcggg acccttgccg tcggtgtcgt taccgtcgca gcgctgtact
     1381 tcggacggga agtcgttgtt ccgatggccc ttgcgatcct tctggggttt gcgctcgcgc
     1441 cgctcgtgct ccttctgcgc cgttggcact tgggccgggt gccatcggta attgcagttg
     1501 tcgtgctcgc cttcctgatg atcgtcggga tcggcgcctt cgtcggcaat cagcttgccc
     1561 acctggccgg ggagcttccg ggataccaaa ccaacattgc acaaaagatc cgttcgcttc
     1621 gggacacgac agccaagggg gcgttgtcga ccgcacctcg gcgatgctga gtaatcttaa
     1681 taacgagatc acggagccag aagaaaagga agggggcagc gtagggaacc aagttggcgc
     1741 tttgccgcac aacaaccaac cgcaagaccc ggtgccggtc gagatacgcc gacacaatcc
     1801 gtcgccgttc ctgctcttcc agcagttcgc cgtacccctg cttcaacctt tcgcgacggc
     1861 cggaatcgtc gtcgttttcg tgatcttctt cctgctgcag cgggaagatc tgcgagatcg
     1921 cttcatccgt ctcgccggcg cgggagatct gcggcgcaca acgctggctc tcgatgatgc
     1981 cgccagccgc ctcagccgtt acctcctgac ccagaccgcg atcaacacga gcttcggcgt
     2041 attaattggc accggactgt ggttcatcga cgtgccccat ccgatgctct ggggcattct
     2101 cgggatgctg ctgcgctttg tgccctatat cggccctgtt atcgccgccg cgcttcccgc
     2161 gatcgtcgca ttcgcggtcg atcccgggtg ggccatgctg ctctgggcga tcgctctgtt
     2221 cctcgtcgta gagctcgcca tcgggcaggt gatcgagcca tggctctacg gccacagcac
     2281 cggcctttcc ggcatcgcgg tcgtcgtcgc cgcggctttc tggacactgc tttggggacc
     2341 ggttgggctg ttactgtcga cgccgctgac gatgtgcctg gttgtcctgg gccggcacgt
     2401 cgagcaatta cagtttcttg aagtgcttct cggcgaccgg cccgcactgg cgccggagga
     2461 agccttttac caacgcatgc tcgccgggga tcccgacgaa gcggcgcacc aagccgaaga
     2521 gttcctccag aacaaacctc tggcagccta ttacgacgaa gtcgcgatcg gggggctcac
     2581 actggctcaa tgggacgtaa atcggggggg cgctgggaca cgagcaccgg gtcctgatca
     2641 aagagtcggt cgaaggggtc atcgacaatc ttgccgacca cgccccgtgg ggtgctcaga
     2701 aagtgggaga gaatggtgag tcggaagtgc cgccagtttt ctcgcaagag gaacttgccc
     2761 ccgtttggcg ggacacagca gtattatgtg ttgccggccg cggatcgctc gacgaggcat
     2821 cggcggcaat gctggcacag cttcttcaaa aacacggcat cggcgcgcgt gtggtgcctt
     2881 ccgag
//
LOCUS       DATAQL010001293         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3949626, whole genome shotgun sequence.
ACCESSION   DATAQL010001293 DATAQL010000000
VERSION     DATAQL010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949626"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VHS97_22295"
     CDS             <1..858
                     /locus_tag="VHS97_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX3451001.1"
                     /translation="GFQLKERPWYDDPYQVIAELCHNKKVATDLGGNGCSPARREGDA
                     LRALRRCLTPLERMRLRELGRTLSAAVEATCRNFDPGEREADVAGHLAHRLIRQGVVP
                     VDLRVASDGRLARFRQPTFKATPIRKRATIAVTGRRHGLCASVTRSVAFGSVGSEFRD
                     HQALATMVDASCIFFSRPGEVISEVFRRAKRIYEKFGHPHEWTLDYQGHVVGYSPREI
                     VLRPDSNLTLESNMALAWSPSVGAARTEDTVIIDSRGYEVITKMQNWPAIEVAVKGYV
                     IPRPGILVR"
     gene            1037..1867
                     /locus_tag="VHS97_22300"
     CDS             1037..1867
                     /locus_tag="VHS97_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3451002.1"
                     /translation="MRLAWKLGRVAGIDFYVHPTFLVVFFLFPGVFPGGALSPLNILL
                     VLTVFGCVLLHELGHALTARRFGINTHDITLYPIGGVARLERMPRAPGAELLIALAGP
                     AVNFAIVLGLIAIFFLGGDLLSPFSWPVLFLQQVLVVNLVLGVFNLIPAFPMDGGRVL
                     RALLSGWLGRLRATMAAARVGRALAVLFGLLSLSYNPIQVALVHVALASFIYMAAKGE
                     EAQVLAEEGRRQGFGAENAGIWTAPPGYYWVRRGNGLWQLAPIGVGFGEPAHPAPPWR
                     "
     gene            complement(1905..3119)
                     /locus_tag="VHS97_22305"
     CDS             complement(1905..3119)
                     /locus_tag="VHS97_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451003.1"
                     /translation="MIDAPAETETAARETHDSETPFTSVEAALRDGGPAAAIGRLTEH
                     LAAAGENRALLDALLLKARLELGLPLVSSGSLAGIPEPERSQYEEKYIAAIRQVGARY
                     LDAGDIPTAWAYYRVIAETEPVAAAIGRYRPDDNDEKLGAVIEVAFNHGVSPERGFEM
                     ILEHYGTCPAITAFDQLPPHDQGARSACAGRLIRHLHRELSANLRSEIANRGQVIPPV
                     GTLIADLLQTRPWLLADESYHIDISHLAAVVRMSLLVDDRELRALAADLTEYGKRLSP
                     RLLFEGPPPFEKIFDDHGLYLKGLLGIEVDQAVAHFRGKVAAGDVDDNEASIPAQTLV
                     NLLNHVGELDAAIDVAAEHLAGLPESALSCPSLAQLCLRAGQPARLALLARAQKDLVT
                     FTAALIQTRSGA"
     gene            complement(3197..>3562)
                     /locus_tag="VHS97_22310"
     CDS             complement(3197..>3562)
                     /locus_tag="VHS97_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX3451004.1"
                     /translation="FATGFVIFPGGFGTLDELFEALTLVQTRKIHRFPIILFGRAYWQ
                     GLLDWITSTQLVEGAVSREDLNLLIVTDSVEEARDMLVDCYHSRCWSTWKHSVGARMD
                     ADPPGAPATALDPGKAAGE"
BASE COUNT          608 a         1096 c         1128 g          730 t
ORIGIN      
        1 ggctttcagc tcaaggaacg tccctggtac gacgaccctt accaggtgat cgccgagctc
       61 tgtcacaaca agaaagttgc gaccgacctg ggaggcaacg gctgctcgcc cgcgcgccgc
      121 gagggtgatg cactgcgggc cttgaggcgc tgtctgaccc cgctcgagcg gatgcggctg
      181 cgcgagctcg gacgcacgtt gagcgcggcc gtcgaggcca cgtgtcgcaa ctttgatccc
      241 ggcgagcgtg aagccgacgt tgccggccac ctggctcacc gcttgattcg ccagggcgtg
      301 gtcccggtcg acctgcgcgt cgccagcgat ggccgcctcg cccgttttcg tcagccgaca
      361 ttcaaggcaa cacccatccg caagcgagcg acgatcgccg tgacaggccg gcggcacggg
      421 ctgtgtgcat ccgtaacccg atcggtcgcg ttcggatcgg tcggctccga gtttcgggac
      481 catcaggcct tggccacgat ggtcgacgca tcgtgcatct ttttttcccg ccccggcgag
      541 gtcatctcgg aagtctttcg ccgggccaag cggatttatg aaaaattcgg ccacccgcac
      601 gagtggacgc tcgattacca aggacatgtg gtcggctatt caccccgcga gattgttttg
      661 cgccccgata gcaacctgac gctcgagtcg aacatggctc ttgcctggag tccgagcgta
      721 ggtgccgcgc gtaccgaaga taccgtcatt atcgacagcc gcggctatga ggtcattacc
      781 aagatgcaga actggccggc catcgaggtt gccgtcaaag gttacgtcat cccccgtccc
      841 ggcatcctag tgcgctagac ttcgctcagt taagtccttt gccagcaatc gcttcactcc
      901 aagtcccccc ttctctgccc accggccatc gatttatcgg ggctcctctt tccatcgaac
      961 tttccctgcc ctgactacga agtacaatag gatatgtaac cgaaggtttt gccttcggag
     1021 gaaggatatc acttcgatgc gtttagcttg gaaactgggc cgggtcgcag ggattgattt
     1081 ttacgtacac ccgaccttcc tcgtcgtttt ctttttgttt ccgggtgttt tccccggcgg
     1141 tgccttatca ccgctgaata tcttgctggt tctgaccgtt ttcggctgcg tgctcctgca
     1201 tgagctggga catgccttga cggcgcgaag gttcggaatc aacacccacg acattacgct
     1261 ctatccgatc ggcggcgtgg cgcggctcga gcggatgccg cgcgctccgg gagcggagct
     1321 attgattgcg ctcgcggggc cggccgtcaa cttcgccatc gttctgggtt tgatcgcgat
     1381 ctttttcctg ggcggcgacc tgctaagtcc attttcgtgg ccagttctat tcttacagca
     1441 ggttttggtg gtgaacctgg tgctcggcgt gtttaacctc atccccgcct ttccgatgga
     1501 cggcgggcgc gtcttacgtg cgctcttgag cggctggctg ggccgacttc gggccacgat
     1561 ggcggccgct cgggtcgggc gcgctttggc tgttctcttt ggcctcctgt cactgtccta
     1621 caacccgatt caggtcgccc tcgttcacgt ggctctggcc tcgttcatct acatggcggc
     1681 caagggcgaa gaggcccagg ttcttgccga ggagggacgc cgacagggat ttggcgcgga
     1741 gaacgcgggt atctggactg ctccgcccgg atattactgg gtccgccggg gaaacggcct
     1801 ttggcaactg gccccgatcg gggtcggctt tggcgaacct gcacaccctg ccccgccatg
     1861 gcgctgaccc aatcacgcta ctgcgtgtag tgtagtgtag cctctcaggc accggaacga
     1921 gtttgaatga gcgccgccgt gaaggtgacg agatccttct gcgcccgtgc gagtagcgcc
     1981 agccgagcgg gctggccggc gcgcaggcac agctgggcta agctggggca cgacagcgct
     2041 gactcaggca gacctgccag gtgctcggcc gccacgtcga tggcggcgtc cagttccccg
     2101 acgtgattca gtaggttcac gagcgtttgg gcggggatgc ttgcctcgtt gtcatcgacg
     2161 tcgccagcag ccacttttcc ccggaaatgg gcgactgcct ggtcgacctc tatgccgagt
     2221 aggcctttca aatagagtcc atgatcatcg aagatcttct caaacggagg aggtccctcg
     2281 aacagcagcc ggggcgagag gcgcttgccg tactcggtca ggtcggcggc cagcgcgcgc
     2341 aattcacggt cgtcgacgag aagcgacatt cgtactacgg ccgcaaggtg cgagatatcg
     2401 atatggtacg actcgtcggc gagcagccac ggtcgagtct gtaggaggtc ggcaatgagc
     2461 gttcccacgg ggggtatcac ctgacctcgg ttggcgatct cggagcgcag gttggcggaa
     2521 agctcacgat ggagatggcg aattaggcgc cccgcgcacg ccgaacgggc cccctgatcg
     2581 tgaggcggca actgatcaaa ggccgtgatt gccggacagg tgccataatg ttccaggatc
     2641 atctcgaatc cgcgctcggg gctgacaccg tggttgaagg cgacctcgat gacggctccc
     2701 agcttttcgt cgttgtcgtc cggtcgatag cggccaatgg cagccgccac cggctcggtc
     2761 tcggcgatga cgcggtagta tgcccaagcc gtcggaatgt cgccggcatc gagataccgg
     2821 gcaccaacct gccggatcgc cgcgatgtat ttttcttcgt actggctgcg ctcgggttcg
     2881 ggtatgccgg cgagggagcc cgagcttaca agtggcaggc cgagctcgag ccgcgccttg
     2941 agcaagagtg catcaagcaa ggcgcggttt tcaccggccg cggcgaggtg ctcggtcaaa
     3001 cgtccaatcg cggcggcggg acctccgtcg cgcaaggcgg cttcgacgga cgtgaagggt
     3061 gtctcggagt cgtgcgtctc gcgagcggcc gtctcggtct cggctggtgc gtcaatcatg
     3121 gctcgagcgg tcctcgtctt ggtgaaaaat accgtcgatt tcacgccgtc gcccgatcat
     3181 ttgccccgcg gatcactcat tcgccggccg ccttgcccgg atcgagcgcg gtcgccggcg
     3241 cgccgggggg gtcggcgtcc attcgggcgc ccaccgaatg cttccacgtc gaccagcacc
     3301 gggaatgata gcagtcgaca agcatgtcgc gagcctcctc gaccgagtcg gtaacgatca
     3361 agaggttcag gtcttcccga gagactgccc cttcgacgag ttgagtcgag gtgatccagt
     3421 cgagcagacc ctgccagtac gctctgccga acaggatgat cgggaaccga tggatcttgc
     3481 gcgtctggac gagcgtcagc gcctcgaaca gctcgtcgag ggtgccaaag ccgccgggaa
     3541 agatgacgaa accggtggcg aa
//
LOCUS       DATAQL010001294         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4253949, whole genome shotgun sequence.
ACCESSION   DATAQL010001294 DATAQL010000000
VERSION     DATAQL010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4253949"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..384
                     /locus_tag="VHS97_22315"
     CDS             34..384
                     /locus_tag="VHS97_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451005.1"
                     /translation="MSTQTAHPDAKDQKDNVHDTPNEYPFVLYNHKTRQTKLAKDKDE
                     YDKLSKAGFEEDPLEPEDPDMLTQDEMKLLRQLFAKAANALEKLARLSQSDDKGNDKA
                     QTAAGQKETAAHRK"
     gene            392..871
                     /locus_tag="VHS97_22320"
     CDS             392..871
                     /locus_tag="VHS97_22320"
                     /inference="COORDINATES: protein motif:HMM:NF024405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEX3451006.1"
                     /translation="MASLDPSSLDASASGALSNLFNVIAGEFSTEWMAIPDDSWLTVI
                     DPVGPVTGNASVLYAVAANEGAARNGSITITGFDLVFSVNQAAAVEEPPVEPPVEHPP
                     LAPIGPGGQSDNASWADLMCRFNTEVAAAQTNPFAPPPTTRTIEKWLRTLTPSSPTL"
     gene            832..1533
                     /locus_tag="VHS97_22325"
     CDS             832..1533
                     /locus_tag="VHS97_22325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451007.1"
                     /translation="MATDINTLLTDALIYVGAYAQGQTANSDDLALAFRVINRKLDSL
                     SAEKLSMVGMHRRQFALTGLASYNFGPGLTWDSSTRPIKIKSASVIAQNGVEKECNLP
                     TADQWAAIPDKTRTGIYVEDLFYDNGFPNGLFYVTPIPFSGSMILWSFEAIAQLAAQT
                     GTVNFAPGYEQTILTIAARELCIAFQRPLTQELSDAAEQSKNVIVQLNAELFNAPMPP
                     PQGPGPTSPPARTTT"
     gene            1534..>2366
                     /locus_tag="VHS97_22330"
     CDS             1534..>2366
                     /locus_tag="VHS97_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451008.1"
                     /translation="MTARPFLMRNFGSVNPLSLGKPAARFPGAIATDSDLAIAVDRQQ
                     TTLAQSLDSVSTTMTVASGSIVTAYNLLSIDAEIVKVTSAPAGNVISIQRGFDGSVPA
                     AHSAGATVSGYVDAWHHNALVAEIEAIESALGPNLSSIPGASPYQMSSAHNFAPQSPG
                     GSLGVGTNQITISPVPQGLNGSDAKHYLLISGGTGAQEAVLITGGSAVAGAPSGTLFI
                     TCANTHSGAWTIGSATAGIQEACIHAVAAAATAGVVWVSGSVTVYAKISVPAGITITG
                     AG"
BASE COUNT          513 a          758 c          686 g          409 t
ORIGIN      
        1 acctgaatcg agatcgaaaa aaggagtaac taaatgagca cccagaccgc gcatcccgat
       61 gcgaaggacc agaaggacaa cgtccacgat accccgaacg aatatccctt cgtcctctac
      121 aaccacaaga cccgtcagac caaactggcg aaggacaagg acgagtacga caagctttcg
      181 aaagccggat tcgaggaaga cccgctcgag ccggaagatc cggacatgct cacgcaggac
      241 gagatgaaac tcctgcggca gttgttcgcg aaagcggcga atgccctcga gaaactcgca
      301 cgattgagcc aatccgatga taaaggcaac gacaaagcgc aaaccgcagc cggtcaaaaa
      361 gagaccgcag cgcatagaaa gtaggagccc tatggcatcg ctagacccgt cgagcctcga
      421 cgcttcagcg tcaggcgccc tgtcgaatct cttcaacgtg atcgccggcg aattttcgac
      481 tgaatggatg gccatcccag acgactcatg gctcacggtg atcgacccgg ttggaccggt
      541 gaccggcaat gcaagcgtac tttacgcggt cgcggcgaac gaaggcgcgg cgcgcaatgg
      601 ctccatcacg atcaccggct ttgatctggt gttttcggtg aaccaggcgg ccgccgtgga
      661 ggagccgcct gtcgagccgc ctgttgagca tcctccgctc gctcccatag ggcctggggg
      721 gcagagcgac aacgcatcat gggcggacct gatgtgccga ttcaacactg aagtcgcggc
      781 tgcgcagact aatcccttcg cgccgccgcc gactacccgg acgatcgaaa aatggctacg
      841 gacattaaca ccctcctcac cgacgcttta atttacgttg gggcctacgc tcaaggccag
      901 accgcgaact cggacgacct ggccctggcc ttccgcgtaa tcaaccggaa gctcgactcc
      961 ctatccgccg aaaaactctc catggtgggc atgcatcgcc ggcagtttgc attgaccggc
     1021 ctcgccagtt ataacttcgg gcctggactg acgtgggatt cttcgacgcg gccgatcaag
     1081 atcaagagcg catcggtaat cgcccagaac ggcgtcgaga aggaatgcaa tctccctacc
     1141 gccgatcaat gggccgccat cccggataag acccgaacgg ggatctacgt ggaagatctg
     1201 ttctacgaca acggattccc gaacggcctt ttctacgtga ccccgattcc gttcagcggc
     1261 tcgatgattc tctggagttt cgaggcgatc gcgcagctgg cggctcagac cgggacggtc
     1321 aatttcgcgc ccggatacga gcagacgatc ctcaccattg cggcaaggga gctctgtatc
     1381 gccttccagc ggccgctgac tcaggaactg agcgacgccg cggaacagtc gaagaacgtc
     1441 attgtgcagt tgaacgcgga actctttaac gccccgatgc cgcctccgca ggggccggga
     1501 ccaacctctc ctcccgcaag gacgaccacc taaatgacag ccagaccatt tctcatgcgg
     1561 aatttcggct cggtgaatcc gctatcgctc ggcaagcccg cggcgcgttt cccgggcgcg
     1621 atcgcgaccg acagcgatct cgcgatcgcg gtggaccggc agcagaccac gctcgcccag
     1681 tcgctcgaca gcgtctctac cacgatgacc gtggcgtccg gctcaatcgt cactgcttat
     1741 aatctgctct cgatcgatgc cgaaatcgtc aaggtgacga gcgcgcctgc gggcaatgtc
     1801 atctccatcc agcggggatt cgacggaagc gtcccggcgg ctcactcggc cggcgccacg
     1861 gtctcgggat acgtggacgc ctggcaccac aacgccttgg ttgcggagat cgaagccatt
     1921 gaaagtgctc tcgggcccaa cctttcgagc attccggggg cctcgcctta tcaaatgtcg
     1981 tccgcgcata actttgcgcc gcagagcccg ggcggatctt tgggagtggg gaccaaccag
     2041 attacgatct ctcccgttcc gcagggactc aatggctcgg acgcgaagca ctatctcctg
     2101 atctcagggg gcacgggcgc gcaggaagcg gttctgatca caggcgggag cgcggtagcc
     2161 ggcgccccca gcggcacgct cttcatcacc tgcgccaaca ctcactcggg cgcctggacg
     2221 atcgggagcg ctaccgccgg catccaggaa gcctgcatcc acgctgtagc agccgcggcg
     2281 acagctggcg tcgtatgggt ttccggatcc gtgaccgtct acgccaagat ctctgtgccg
     2341 gcgggcatca cgatcacggg cgcggg
//
LOCUS       DATAQL010001295         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2880070, whole genome shotgun sequence.
ACCESSION   DATAQL010001295 DATAQL010000000
VERSION     DATAQL010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880070"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VHS97_22335"
     CDS             <1..799
                     /locus_tag="VHS97_22335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX3451009.1"
                     /translation="GRRGGGRKSREPLQAGDPVFSSIFDLGAGALAEFALVPENAAAL
                     KPANLDFVQAASIPMVGLTSWQALKDRAHLKPGQKVFIPAGAGCIGTFAIQLAKYLGV
                     TVATTTSAGKVDLVRSLGADEVIDYKKQQFEDVLRGYDAVLGTVRGDAIEKSLRILKP
                     GSTVVSLVGPPDAAFARARGMNFFMVFVFGLLSRKIIRRAREGGVAYSFLFVHPDGDR
                     LAEIGELLNSGRIRPVIDKVFPFEQAKQALAYLEKGRAKGKVVVQMR"
     gene            978..1379
                     /locus_tag="VHS97_22340"
     CDS             978..1379
                     /locus_tag="VHS97_22340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3451010.1"
                     /translation="MTPLEKIIAMKLPFAELKGVTFTEASQDKVVARMTVRPDLCTLG
                     HVLHGGAAMALADSVGAAATVINLPEDAKGTTTLESKTNFIGPAKEGATVIATATPIH
                     RGRRTQVWQTRLETEEGKLVAVVTQTQMVLV"
BASE COUNT          329 a          489 c          544 g          318 t
ORIGIN      
        1 cgggcgtcgt ggtggaggtc ggaagtcgcg tgagccgctt caagccggcg atcccgtgtt
       61 ctccagcatc ttcgacctgg gcgcgggcgc tctcgccgag tttgcgcttg tgcccgagaa
      121 cgccgctgcg ctcaaaccgg caaatctgga cttcgtgcaa gcggcatcga tcccgatggt
      181 cggattgacg tcgtggcaag cgctcaaaga ccgagcacat ctcaagcccg gtcaaaaagt
      241 tttcatcccg gcaggcgcgg gctgtatcgg cacgttcgcg atccagctcg cgaaatacct
      301 cggggtgacg gtcgcgacca cgacaagtgc ggggaaggtc gatctggtgc ggagcctcgg
      361 agccgacgag gtgatcgact acaagaagca gcaattcgag gacgtgctgc ggggctacga
      421 tgccgtgctg ggtacagtga ggggggacgc gatcgagaaa tcgcttcgga tattgaagcc
      481 ggggagcacc gtcgtctcgc tcgtcgggcc accggacgcc gcattcgccc gtgcccgagg
      541 catgaacttt ttcatggtgt tcgtgttcgg gttgttgagc cgtaagataa ttcgtcgggc
      601 cagggaaggc ggcgtcgcgt attcgtttct gttcgtgcac ccagacggtg atcgactcgc
      661 agagattggt gagcttctca actcagggcg cattcggccg gtgatcgaca aggtgtttcc
      721 gttcgaacag gcaaagcagg cgcttgcata tctcgagaag gggcgagcca agggcaaggt
      781 cgttgtccag atgaggtaat ggcgtcgaca tgggcgctgt cgactccggt gcctgaactg
      841 acaaccccgc ttggccgcga agccgagccc gccgtcacat caccataacc aagtcaccgc
      901 cgtctttacc tccgacgtca ttgcctgact tacccggcag cttctatcct caggccaaag
      961 ccgaaacggg aggacgcatg acgccgctgg aaaaaatcat cgctatgaag ctgccctttg
     1021 ccgagctgaa gggcgtgacc tttaccgagg cgagccagga taaagtggtg gcgcggatga
     1081 cggtgcggcc ggatctctgc accctcggcc atgtccttca cggcggtgcg gccatggcgc
     1141 tggccgattc cgtgggcgcg gccgcgactg tgatcaacct gcccgaggat gccaagggca
     1201 ccaccacgct cgaaagcaag accaacttca tcggcccggc gaaggagggc gcgaccgtga
     1261 tcgcgaccgc aaccccgatc catcgcggcc ggcgcaccca ggtctggcag acgcggctgg
     1321 agacggagga gggcaagctg gttgcggtcg tcacccagac ccagatggtg ctggtatgaa
     1381 ggtagcggcc gccgcccatt agcccagtgt tccgcgagca tttgaggcct accgtggcct
     1441 tgatttccat ggtgcaatgc acaatatggt ttgtgctagg gattcgacgc ttcctcgagg
     1501 gctatccaag aggagtattg atatgccata ccatgatacc tacagcgcgc aaccgggctt
     1561 tggctatgtg cggactctga gtcagcggga agagttgccg ctgttgcgcg atcacctgct
     1621 gcggctcgat acggaaagcc ggcacgaccg cttcagcgga tatctcgacg acgagttcat
//
LOCUS       DATAQL010001296        16009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1049248, whole genome shotgun sequence.
ACCESSION   DATAQL010001296 DATAQL010000000
VERSION     DATAQL010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 16009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16009
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049248"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VHS97_22345"
     CDS             complement(<1..1413)
                     /locus_tag="VHS97_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX3451011.1"
                     /translation="MNLLHQPWLELAVVITLLGSFGVSRVRHPERVYGWSVAFIGASF
                     GCTVLAWLAFYVGVPAESLARRSVQPLLFGRQIFVLDELNAPILPTVALLHLLVALST
                     SRTFMRRFSFSWSMAAETIRLLTFSCKEPWVLIALLAISTLPPYVELRNRRRSTRVYI
                     LHMALFVGLLIVGWSAVTLGGGPQGAAPWWAAVPLLVAVLVRCGTVPVHCWVTDWFEH
                     ASLGIAVLFVVPLSGVYVAVRLVLPIGPGWVLQSIGLLSLVTAVYAAGMAVIQREARR
                     FFAHFFLSHSSLVLVGLELHTSLSLTASLCLWFSVILSLGGFGLTLRALEARFGRLSL
                     SDFHGLYEHAPTLAVCFLLTGLASVGFPFTLGFISTELLVDSAVEASPYVGIIVITAG
                     ALNGIAVMRAYFLLFTGARHVSTVSLGIGLRERVVVLSFSALILGGGLFPQPGVATRR
                     RAAEEILQDRGKHLHLDQPGP"
     gene            complement(1410..2798)
                     /locus_tag="VHS97_22350"
     CDS             complement(1410..2798)
                     /locus_tag="VHS97_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX3451012.1"
                     /translation="MTYEPLLTLLGLTVVVSPLLLTIVLGVASFLDWKLPEKTTANLV
                     FVTIVSGLTAAASVLLLMLTTGTRHVAIHVGDWVTIPYLYHFSVKFEFDRLSVPFAIL
                     SFVLAGTIAAFATKYMHRERGFNRFFMLYALFVLGMAVTSLAGTIETLFVGWELVGLS
                     SALLVAFFQERPAPARNGLWVWTIYRVSDASLLLASVVMHHLRGEGDFDKLLGSGPWP
                     DQISAVTTAQALVIGMLLVVAAAGKSALVPFSGWLPRAMEGPTPSSAVFYGALSVHLG
                     AFLLLRVSPLLDISTILCVVIVVLGLSTAVLAYLAGTVQTDIKAALSFASLSQVGIIV
                     AEIGLGFRYVALVHLLGHASLRTLQFVRAPSLLQDYRTIEDAIGEHLPRTNTLWYRIA
                     TRHDLGWLYKVALERGYLDRALLQYVVAPFVRTFRWCDALERRWTDFLAGEASRESDL
                     VRPRFGTIEEFS"
     gene            complement(2833..5853)
                     /locus_tag="VHS97_22355"
     CDS             complement(2833..5853)
                     /locus_tag="VHS97_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEX3451013.1"
                     /translation="MIGHASHVLPAQGPITVFIHHNTLHAFEDLPFHEAVKKGAQVFG
                     CHPYLSEDRYRDALARGRIRFSDLQDVLEEDLGTRAGEEIPCFGNRIGLWLAMLQYPL
                     RSGPTEELVWYVAEANALRRVRSEVSSAVRSRLIAETRRWVIRDLRAGNGRATRSKPA
                     EQVSTSLAQLLTRFGEAPIETWTDEDWEGFTLQALWRLCCDGVRDLPPYTTPPPPPLR
                     QRDLLFEATGVDTDAMVHDVMIRFCAAFLDQGMASWQLPRRNEGFFRAFCALYRRPGW
                     APTDWMRGLAQELGRLDDEGVGPVDSIMESLDALGVSEEEWERFLAATLLALRGWGGM
                     IHQVELHGDRVVQPIPEGSMAGFLAIRLLLDRFALAYTARTTLGIHAPVREFWSLARG
                     KHDPHWPPSVEQRAFLVFQLAQVLGLSPDAIYRLTKPEWAILVREIESFSGLERRRIF
                     HLAYERRFCTQTLDAIALHARRPTSRPESPRFQAVFCIDEREESFRRHLEELAPDVET
                     FGVAGFYAVAIYYKGAADAHFTPLCPVVIRPGIWVTEEVQTELGEAHRRRAQTRRVLG
                     TVSLRIHEGSRGFALGAVLTGALGVLASFPLLARILFPRLTAQIRHKFGRLVQTPPLT
                     RLRIERTEPSPGPADTAIGFSVAEMIDAAERLLRDIGLTRGFARLLLLHGHGSHSQNN
                     PHDSAHNCGACGGGLGGPNARTMAQILNDQRVREGLAQRGLSLPVDTVGVGGYHNTCD
                     DSVTYYDLDRIPASHLQEFEKAREEIELTCDRNAHERCRRFYSAPLALSFAAAREHVE
                     GRAEDLAQVRPEFGHATNAITIVGRREWSRGLFLDRRAFLTSYDPTEDDADRTILTRV
                     LQAVFPVCSGINLEYYFSNVDNVGFGCGTKLPHNLASLLGVMDGAASDLRTGLPWQMV
                     EIHEPVRSQFVIETTPEAMLRIMDRNPGIGRLCKNGWVLLAVIDPETRALSVFEDGVF
                     RPYKPQASVLPRAASSVDWYRGWRDHLEFAEIES"
     gene            6886..7425
                     /locus_tag="VHS97_22360"
     CDS             6886..7425
                     /locus_tag="VHS97_22360"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX3451014.1"
                     /translation="MSRLHAFSIPSSERLILSERETMGRNDPFKLVFLITLAVLPVAI
                     LKMQPALRENYLPITACFVLGVLLLLSWWLDCLGHRLIVTDRRTIFRKGLLSKFTNEV
                     LHTHFRNIQISQTFFQRLMHVGSIGISSSGQAGIEIFIRGIPNPYDVKTIIDRYRFGD
                     STVDSDAVHDPQQYQLPVA"
     gene            7579..8067
                     /locus_tag="VHS97_22365"
     CDS             7579..8067
                     /locus_tag="VHS97_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451015.1"
                     /translation="MQQVQPAFIMFAQQSQQAWIIAQHSLSPLVQVKQTPMSIASHLH
                     IPIIMLQQQTIIPFIIIQQLHIPPAIIVHRFWSIPADVCSSQTQLIFIPPLDFSNVIL
                     QRGTIIILGAAGVVADAPVIPMPGMPIRFRSVIMVAIVQVLSLTLNRHSNSADPSNLL
                     RF"
     gene            8778..10166
                     /locus_tag="VHS97_22370"
     CDS             8778..10166
                     /locus_tag="VHS97_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451016.1"
                     /translation="MKKPESSRRMSRVPHLSAGLFLSLMGIVYLFCASVHGQLTLRDQ
                     GFVPFRDEPINYLSDRVSDPVAKLQQRIDQGQVALDFDPKHGYLESVLRFLKVPASSQ
                     TLVFSKTSFQYKKISPCSPRALYFNDNVYVGWVQGGHSLEIASFDPDQGAIFYLLDQQ
                     PSDQPAFVRATLDCTQCHVAPGTRGVPGVLLRSIFARPSGTQAIETDSFITGHETPFK
                     DRFGGWYVTGKHGRQLHMGNVFVEDTAHPERLNRSAGANMTDLSQRFPSATYLTPHSD
                     IVAHLVLAHQTQMHNLITLVNYKTRLALHADRAISKSGGHPDRSIPETTRGKIEAPAE
                     ELLSYLLFADEAPLETQIEGTSGFSQEFAATGPRDREGRSLRDFDLKRRIFKYPCSYL
                     IYSEAFDALPAPAKEFIYKRLLAVLSGRDQSGRYNRLTPGERRDILEILLATKPGLPD
                     EWKRLGTAPVAK"
     gene            10219..11454
                     /locus_tag="VHS97_22375"
     CDS             10219..11454
                     /locus_tag="VHS97_22375"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3451017.1"
                     /translation="MRKLVCLGILLTFGPLARGESVTGRWDATVTIKDAEIPFRIDFS
                     GEGTQFTGTVFNGDLPVSSTSGRLENGKLEVSFGHYLSRLDATVKDGQIEGKLSGRFE
                     RDRYISSYPFRAQRYTPLAATSENVPEIDGIWEIPYNSPKGEKSWHFIVRQKGPEVAA
                     AILRVDGDTGTLTGTYKDGRFSVSHFSGSRPLRLEITPGRNNTLEIVPSGAYTAKEKL
                     VAYRPADARAKGLPEPTDPDTHTRVKKPADVFKFSFPDVDGNVVSNTDSRFVGNVIVA
                     VVTGTWCPNCHDEAQLLVELDRKYRDRGLRVVALDFEEPEQQEDGLKRARSFIKKYGV
                     EYPYLIAGAPIEMWEKVPQAENLNSWPTTFFVGRDGRVKLIHAGFAAPASGEFHRKVR
                     EEFISTIERLLAENTTAAQ"
     gene            11848..12240
                     /locus_tag="VHS97_22380"
     CDS             11848..12240
                     /locus_tag="VHS97_22380"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-X9-DG-CTERM domain-containing protein"
                     /protein_id="HEX3451018.1"
                     /translation="MKRELGAPATHLDCHDETGGPLVFRSRDRSTRRSRSFDHSIDTF
                     ESAASSFHPGGVNVGFADGSVRFIKDSVSTLAFSQSTGLPNALIATPNGNCTNSAPIY
                     SFQPGTQLGVWQALSTRAGGEVVSADSY"
     gene            12303..13577
                     /locus_tag="VHS97_22385"
     CDS             12303..13577
                     /locus_tag="VHS97_22385"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX3451019.1"
                     /translation="MSRFVPSRAQWRLVLHTLVIAASVTVLASRAIAEDPALVDIEQD
                     AKRIHENVLVLDSHVDIPVDYGTGRNDPGIDGSTQVDLPKLERGGVDAAVFAVFAKQD
                     KRTPGGLLKAKAEADRKLKAILDIPRRYPHQAALALSAADVERIHREKKVAVIVGFLN
                     AASLGTDLSLIDSYYRAGIRTFGFVHAGNNDFADSSRPIGDDKYGEHGGLSSLGREAV
                     TRLNRLGVILDVSQLTPEALLQSVKLSKAPVVATHSAIKALVDSPRNLSDTELDAIKA
                     NGGVVQIVAFSYYLKAPRFDLASKYKELAARYKKDTRELSKEEDEELHRELYALAPRD
                     ATVDDLVNAITYAVKRIGIDHVGISSDFNHGGGIVGWKDESEALNVTAELLRRGYAED
                     QIAKLWGGNFLRVFRQVENVSEQLRLADSRRP"
     gene            13754..14002
                     /locus_tag="VHS97_22390"
     CDS             13754..14002
                     /locus_tag="VHS97_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451020.1"
                     /translation="MQATAPRTSFRIMADGEMIAAADSINAIADSVRYFHPDIYQVEE
                     VIDGNRGAGRRAFRFWGWVTHRDDGIVAVEPCLSVLEG"
     gene            14486..>16009
                     /locus_tag="VHS97_22395"
     CDS             14486..>16009
                     /locus_tag="VHS97_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX3451021.1"
                     /translation="MFALRTRTSFLATALASLIASGSATAADSPRESAVLPADQSAAL
                     DRITADSLRGHLSFIASDLLEGRETPSRGLDLAAEYIAAQFRRAGLQAVGDDGYFQTA
                     NWTMLEPDADGFAFEIKGPQAPPIQVVKEHVSLQRLRGFDVATDGIFKIDAQDAKSLE
                     TLKPADVEGQAVFAELAGAGSRDRARFAAVTAFMNRMTALKAKLVVNVSSQPTAATGL
                     APGRPVDPENPRTNDAARAERPGPPLVTVHDSKVFALFRSLPLGPSNKTLVSIHLGEP
                     VSRPIKLRNVVGLLRGSDPALKDTCVIVTAHYDHIGSRGAAGDGSDHIFNGANDDGSG
                     TVSVVEIASALALLKNRPKRSILFMTFFGEEKGLLGSRYYGRHPVVPIEKTVADVNLE
                     QVGRTDDSEGPRIGAATMTGHGYSDLTNVFTAAGQAEGVRLERHPQFSDAFFARSDNQ
                     ALADLGVPAHTISVAFMFPDYHGLTDHWQKIDYANMAKVDRMVARGILMLADNPVEPK
                     "
BASE COUNT         3405 a         4972 c         4681 g         2951 t
ORIGIN      
        1 cgggccgggt tgatccaggt gcaagtgttt cccgcgatcc tggagaatct cctcggcggc
       61 gcggcgccgt gtggcgacgc ccggttgcgg aaacagccca ccgcccaaaa tcagtgcgga
      121 aaacgacagc acaacaacgc gctcgcgaag tccaattcct agcgacacgg tcgagacgtg
      181 gcgggcgccg gtgaaaagta agaaataggc ccgcatgacc gcgatcccgt tgagcgctcc
      241 cgccgtgatc acaataatgc cgacgtacgg gctggcctcg accgcgctat ccaccaatag
      301 ctcggtcgag ataaagccga gcgtaaacgg gaacccgacg cttgccagcc ccgtaagcag
      361 gaagcaaaca gccagcgtgg gtgcgtgctc gtacagtccg tggaagtcag aaagcgagag
      421 tcggccgaat cgcgcctcca gggcccggag tgtcaggccg aatcccccga gtgacagaat
      481 taccgaaaac caaaggcaaa gggaggccgt tagcgacagg ctggtatgca attccagacc
      541 gaccaagacg agcgacgagt ggctcaggaa aaaatgcgcg aagaaccgcc gggcctcgcg
      601 ctgaatgacc gccataccgg ccgcatagac agccgtcaca agcgacaaga ggccgatcga
      661 ttgcaaaacc cagcccggcc cgatcggcaa cacgagccgc accgcgacat acactccgct
      721 gagcggaact acgaagagta cggcgatccc gagcgaggcg tgttcgaacc agtcggtcac
      781 ccagcaatgc accgggaccg taccgcaccg caccagcacc gcgaccaaga ggggcacggc
      841 tgcccaccac ggcgccgccc cttgaggacc gccgcccaag gtcacggccg accatcccac
      901 gatgagcagc cccacaaaga gggccatgtg caagatgtac acgcgcgtcg accggcggcg
      961 attacgcagc tccacgtacg gcggcagcgt agagatcgcc aggagcgcga tcaagaccca
     1021 cggctccttg caactgaaag tcaacagtcg aatcgtctct gcggccatcg accacgagaa
     1081 cgaaaatcgt cgcatgaacg tccgcgaagt ggacagggct accagaagat gcaagagcgc
     1141 caccgtcggc aggattggag cgttcagctc gtccaacaca aagatttgcc ggccaaaaag
     1201 gaggggctgg acgctccgac gcgcgagtga ctcggcgggc acgccgacgt aaaacgccag
     1261 ccacgccagc accgtgcagc caaacgacgc gccgatgaac gcgacgctcc agccgtagac
     1321 acgctcggga tgccgcaccc ggctgacgcc gaaggagccg agcaacgtga tcacgaccgc
     1381 cagttccagc cagggctgat ggaggagatt catgaaaatt cttcgatcgt tccgaagcga
     1441 ggcctgacca ggtcagactc gcgcgaggcc tcgcccgcga ggaagtccgt ccaccgccgt
     1501 tccagggcgt cacaccaccg gaaggtccga acgaacggcg cgacgacata ttgcagcagt
     1561 gccctgtcta agtagccccg ctcgagcgcg actttataaa gccagccaag gtcatggcga
     1621 gttgcgatgc ggtaccacag cgtattggta cggggcaaat gctcgccgat agcgtcttcg
     1681 attgtgcggt aatcctgcaa aaggctcggc gctctgacga actggagagt ccgcaggctg
     1741 gcgtgcccga gcaggtgcac gagagccacg taccggaacc ccaggccgat ttcggccaca
     1801 atgatgccca cctgcgagag cgacgcaaac gaaagcgccg ccttgatgtc cgtctgcacg
     1861 gttcccgcca ggtatgcgag tacggccgtc gacaaaccta agacgacaat caccacgcag
     1921 aggatggtgg agatgtccaa cagtgggctg acccgtaaga gcaggaacgc tcccaggtgc
     1981 accgacagtg cgccataaaa tacggcgctc gagggagttg gcccttccat cgcccgcggc
     2041 agccagcccg aaaaaggcac gagcgccgac ttgccggcgg cggcaaccac cagcaacatc
     2101 ccgatcacaa gcgcctgggc agtcgttacg gccgagattt gatccggcca ggggccggac
     2161 cctaagagct tgtcgaagtc tccctcgcct cggagatggt gcatgaccac cgacgccagc
     2221 aagagtgacg cgtccgagac acggtagatg gtccaaaccc agagtccatt gcgcgcggga
     2281 gcgggccgct cctgaaaaaa cgcgaccaga agcgccgacg acagccccac caactcccat
     2341 cccacaaata gcgtctcgat cgtgccggca agcgaagtca ccgccatgcc caacacgaag
     2401 agcgcataaa gcatgaaaaa ccgattgaat ccgcgttcgc gatgcatgta cttggtggcg
     2461 aacgccgcga tcgttccggc gagcacaaaa gagagaatcg caaacggaac ggagagccgg
     2521 tcaaactcga acttgaccga aaagtgatac aggtatggga ttgtgaccca gtctcccaca
     2581 tggatcgcga cgtggcgcgt ccccgtggtc aacatcaaga gcagcacgct tgcggcagcc
     2641 gtcagccccg aaacgattgt cacgaagacg aggttggcgg tggtcttctc cgggagcttc
     2701 cagtccagaa acgacgcgac gcctaggacg atcgtcaaca aaagtggact gacgacgacc
     2761 gtcaggccca agagcgttaa cagcggctcg taagtcattt ccctcgtctt tccgtagctt
     2821 gcaccagcgc tttcaactct cgatctccgc gaattcgagg tggtcgcgcc agccgcgata
     2881 ccagtcgacg gaggaggccg ccctgggcag caccgaggcc tgaggcttgt acggacggaa
     2941 caccccgtcc tcgaaaacgc tgagagcgcg cgtctcgggg tcgatcacgg ccaagaggac
     3001 ccagccgttc ttgcagagcc ggccgatccc tggattgcgg tccatgatcc gcagcatggc
     3061 ctcaggagtc gtctcgatca cgaactgcga gcgaacgggc tcgtggatct cgaccatttg
     3121 ccagggtagg cctgttcgca ggtcgctggc ggcgccgtcc atcaccccca agagcgacgc
     3181 caggttgtgc ggtaacttcg tgccgcaacc aaagcccaca ttgtcaacgt ttgagaagta
     3241 atactcgagg ttgatcccag agcaaacggg gaatacggcc tggaggacgc gcgtcagaat
     3301 cgtgcggtcg gcgtcgtctt cggtagggtc gtaggatgtg aggaacgcac ggcggtcgag
     3361 gaacaggccg cggctccatt cgcgccggcc tacgatggtg atcgcgttgg tcgcgtgccc
     3421 aaactcgggc cgcacctgtg ccaggtcctc cgctcgcccc tcgacgtgct ctcgcgcggc
     3481 ggcgaacgaa agcgccaagg gcgccgaata gaaccgccgg caacgctcgt gggcgttgcg
     3541 atcgcaggtt aactcgatct cctcgcgcgc cttctcgaac tcttgcagat gcgatgcggg
     3601 aatccggtcc aggtcgtaat aggtcaccga gtcgtcacac gtattgtgat agccgcctac
     3661 accaaccgta tcgacgggga gtgacaaccc gcgctgggcc aggccctcgc gcaccctctg
     3721 gtcgttcaag atttgggcca tcgtccgagc gttcggcccg cccaagccgc ctccgcaggc
     3781 cccgcagttg tgggccgagt cgtgcggatt gttctggctg tgcgaaccgt gcccatgcag
     3841 cagcaacagc cgggcgaacc cacgggtcag cccgatgtcg cggagtagcc gctcggccgc
     3901 gtcgatcatc tccgcgacgc tgaagccgat cgcggtatcg gcaggacctg ggctgggctc
     3961 cgtgcgctcg atgcgcagcc gagtcagagg cggtgtttga acaagccggc cgaacttgtg
     4021 gcggatctgc gcagtcagtc ggggaaagag aatgcgggcc agaagcggaa acgaggcgag
     4081 caccccgagc gccccggtca agacggcgcc cagcgcaaag ccacggctac cttcgtgaat
     4141 ccgcagcgaa accgttccca gcacccggcg cgtttgcgcc cggcggcggt gcgcctcgcc
     4201 gagctcggtt tggacttcct cagtgaccca gattccgggc cggatcacaa cggggcaaag
     4261 cggcgtgaag tgagcgtcgg ctgccccctt gtaatagatt gcaacggcgt agaagccagc
     4321 cacaccgaac gtctcgacat ctggagcgag ctcttccaag tgtcggcgga acgactcctc
     4381 gcgctcgtcg atgcagaaaa cggcctggaa ccgaggcgat tcgggccggc tcgtcggccg
     4441 tcgcgcgtgg agcgcgatgg cgtcgagcgt ctgtgtgcaa aatcggcgct cgtaagccag
     4501 gtggaagatt cgccgccgct ccaatcccga aaacgactcg atttctcgca cgaggatcgc
     4561 ccattccggc ttcgtgagac ggtagatggc gtcgggcgag agcccgagca cctgcgcaag
     4621 ctggaaaacg aggaaagctc gctgctcgac ggagggcggc caatgcgggt catgcttgcc
     4681 gcgcgccagg ctccagaatt cgcgcaccgg ggcatggatt cccagcgtgg tgcgtgcggt
     4741 gtaggcgagg gcgaaacgat cgagcaaaag ccggatcgcc aggaaacccg ccatacttcc
     4801 ctccgggatc ggctgaacga cccggtcgcc gtggagctca acctgatgga tcatcccccc
     4861 ccagccccgc agagccaaca gcgtggccgc gaggaacctc tcccactcct cctcgctgac
     4921 ccccagcgca tcaagcgatt ccatgatcga atcaacgggc ccaactccct cgtcatcaag
     4981 ccggccgagc tcctgagcga gcccgcgcat ccagtcggtg ggcgcccagc ccggacggcg
     5041 ataaagcgcg cagaaggcgc ggaagaatcc ctcgttccgc cgcggtaact gccagctggc
     5101 catcccctga tcgaggaacg ctgcacaaaa ccggatcatc acgtcgtgca ccatggcatc
     5161 ggtgtcgaca ccggtggcct cgaacagcaa gtcccgctgc ctcaaggggg gcggcggagg
     5221 cgtcgtatag ggaggcaggt cgcggacgcc gtcgcaacaa agcctccaaa gagcttgaag
     5281 cgtaaatcct tcccaatctt cgtcagtcca ggtctcgatc ggggcttcgc cgaagcgggt
     5341 caagagctgg gccaaactag tggaaacctg ttctgcaggt ttgctgcgag tggccctgcc
     5401 gttgccggcg cgcagatcgc ggatgaccca ccgccgtgtc tccgcaatca gccgggaacg
     5461 aactgccgac gacacctcgc tacgcacccg ccgtagcgcg ttggcctcgg cgacgtacca
     5521 gaccagttcc tccgtcggcc ccgatcgcaa cgggtactgg agcatcgcca gccagagtcc
     5581 aatccggttg ccgaaacaag gaatctcctc gccggcacgc gtgcccaggt cctcttcgag
     5641 tacgtcttgc agatcggaaa accggattcg cccccgcgcg agtgcatcgc gataccggtc
     5701 ttccgacagg tacggatgac acccgaagac ctgagcccct ttcttcacgg cctcatgaaa
     5761 cggcagatcc tcaaacgcat gcagcgtatt gtgatgaatg aaaaccgtga tcggcccttg
     5821 cgcgggcaac acgtgtgagg catgcccgat cacctcttcc aagcgacgta cccgcgttgc
     5881 ctgatccccg cgtcccagcg aagcgctttt ctgggatgca gaggcttgca cggagtgatg
     5941 gcctcaattg gagtcgttca taagaccgag ggatcggcac atcggagggg ggcaagccgc
     6001 ggaggatgac gctggggcat gcctgtgccg acggcactgc atccacgaac gacgaagggg
     6061 attggacaaa aatctatcgt ggcccatctg agcaaacacg caccaggggc ccgtgtgcaa
     6121 ccaaagtttt ccggcatgaa ttacggcaaa cgttcatata agcatggcga gcaacaaatt
     6181 gcgccggcgt ggcttgtgca aaaaaagatt ttaagggcac gtggagtttg aggcaagtgg
     6241 attaagggac attgcggtgc ccgcccgaga taatacccag cccgcgatag cgagcgcttt
     6301 gcgtcttcgt ccaacagcac cagcgtcgcg gccagcatgc cgtgcttgac ggcattgagc
     6361 tttgcgcgct cttcgccgga ctgggtgcga gggccacaac tcctaagcgc attgcggcga
     6421 ttcgctcggc ttcgacggcg aagaaggtaa tctcggtaac gcgactatgg ccgtagggga
     6481 tatgaaaaat gccgaccgaa ggaggtcccg acaagggaat tccgactgag ggaatcgctg
     6541 gtcggcggca tgcgttgcat gtccttggct ccgtcatctc gtaagcgatt atctgcgtgt
     6601 ctacgggctt gtcgtcggcg atcgagacgg gcagagcagc ggcgcgtcga ccgtaagcat
     6661 ccgaattcgt aggcgcccct attttcgctc tgattccggc acgcaataat atgaggcaat
     6721 aagtggggcg acgctgacgc tcaaaaccgt cgcggagtga tgtgcgcaaa gtcgtggctc
     6781 aaagccaatc gtggcatcta cggttgcgca gatctagttg ggctttgcag cgagcgtctc
     6841 actgctgggc caccctattc gcaagagaag tgcgaggaga atcgcatgtc tcgcctgcac
     6901 gcttttagca tcccgagctc ggaacgactc atcctcagcg aacgggaaac gatgggtagg
     6961 aacgacccat tcaagctcgt attcctaatc acacttgccg tgcttccggt cgcgatcttg
     7021 aagatgcagc cagccctccg agaaaactac ctgccgatta ccgcgtgttt cgtgctggga
     7081 gtgctcttgc tgctcagttg gtggctggat tgcctgggac atcggctcat cgtgaccgat
     7141 cgaagaacaa tattccgcaa aggtctcttg agtaagttta caaatgaagt attacatact
     7201 catttcagaa atattcagat tagccaaaca tttttccagc ggttgatgca tgtcggaagt
     7261 attggcattt caagttctgg ccaagccggc atcgagatct tcatccgagg cattccgaat
     7321 ccgtatgatg tgaagacgat catcgatcga taccgatttg gtgattcgac ggtggacagt
     7381 gacgccgtcc acgacccgca gcagtaccag ctcccagttg cttagcgatg ccgtcatcgc
     7441 catcactggc gatccatccg attaaaatag agcgacccag atcagactta cggctgatcg
     7501 aggtcgtcat ttcaaccatc gccgcaccgc acgccttttg atcagcagct acagcaaacc
     7561 ggcataccat tcatcatcat gcagcaggtg cagccggcct tcatcatgtt cgcgcagcag
     7621 tcgcagcagg cctggatcat tgcgcagcat tccttgtcgc cgcttgtgca agtgaaacaa
     7681 actcccatgt cgatcgcttc acacttgcac atacccatca tcatgttgca gcaacaaacc
     7741 atcattccgt tcatcatcat acagcaactg cacattccgc ccgccatcat cgtgcacaga
     7801 ttctggagca ttccggcaga cgtttgctcc tcgcaaacac agctcatctt cattccgcca
     7861 ttggacttct caaacgtcat cttgcagcgg ggaaccatca tcatattggg cgcagcggga
     7921 gtagtcgccg acgctccggt catccccatg cccggcatgc ccatcaggtt tcgctcagtc
     7981 atcatggtcg ccattgttca agtcctttct ttgacgctca atcggcacag caacagtgct
     8041 gacccctcga atctgcttag attttgacac gccagactct tcggatcaat acctcagtag
     8101 aaaattactt gattatgatc atggcaaagt cgatcaggat tgtgatgtag gtcagcttgg
     8161 gcggacgtcg tcggcgatcg cgctggattt gtgagttggt ggcggaccat catcaagtgc
     8221 gaatcgcttc cgcaaccaat tggtctaaaa tcgcttagga cgcgcatcag gtccgcgtcc
     8281 ctcggagtcg gtgcagccgc tcggcacagg ctctctttga cctgcgcacg catcataaag
     8341 tcgatagtga ttgttgagaa tcggcttggc aggccagagt gtgagccaga cagctttgac
     8401 gccaggttcg aaagcctgaa ctacttgccg tccacgtcga cgctatcggc gacgatgtcc
     8461 caatgtaaat tggatttcga gatgctgtcc caatccctcc tttggcaaat cagacccaac
     8521 acggagcgct ccctgcgaag tccggcgcca atagtcgact cgcccaccct gttaactggc
     8581 cagttccaga aaaagctatt gacatcgttt tgattatgtc gatagactta gttgacattg
     8641 ctgacgggtt tccctaaaat tgcgggcatc tgcggagttt tgaggactga ggttaaattc
     8701 ctcggattta cttgactaca tcgatcgaca tgattgggta cggacggcaa tagccgagtg
     8761 catggagcaa acattagatg aagaagcctg agagcagtcg gcgcatgagt cgcgtgcctc
     8821 atctgtcggc aggtctgttt ctgtcgttga tggggattgt ctacctgttt tgtgcgtccg
     8881 tccacggaca gttgacgctg cgcgatcagg ggttcgttcc gttccgcgac gaaccgatca
     8941 attacctgtc cgaccgcgtc agtgacccgg tcgccaagct gcaacagcga atcgatcaag
     9001 gtcaagtggc actcgatttc gatcccaagc acggatacct cgagtcggtt ctcagattcc
     9061 tcaaggtgcc cgcgtcgtcg cagaccttag tcttctcgaa gaccagcttt cagtacaaga
     9121 aaatctcgcc ttgctctccc cgagcgttgt acttcaacga caacgtctat gtgggctggg
     9181 tccaaggcgg tcattcgctc gagatcgcct cattcgaccc cgaccaaggg gcgatctttt
     9241 acctgctcga ccagcagcca tccgaccagc cggcctttgt gcgggctacc ctcgattgca
     9301 ctcagtgcca tgtcgcaccg ggaacgcgcg gcgttccggg cgtgctgttg cggtcaatct
     9361 ttgctagacc gtcgggaaca caagcgatcg agaccgactc attcattacc ggtcacgaga
     9421 ccccgttcaa ggatcgcttc ggaggctggt atgtaaccgg aaagcacgga cggcagctcc
     9481 acatgggcaa cgtcttcgtc gaggataccg cccatcctga gcggctcaat cgaagtgccg
     9541 gcgccaacat gaccgacctg tcgcaacggt tcccgagcgc gacgtatctg actccccaca
     9601 gtgacatcgt ggcgcacctg gtgctggctc accaaactca gatgcacaac ctgattaccc
     9661 tggtcaacta caagactcga ctggccctcc acgccgatcg ggccatcagc aaatcaggcg
     9721 gacatcccga tcggtcaatt cccgagacaa cccgcggcaa gatcgaggcc ccagccgaag
     9781 aactcttgag ctacctgctg ttcgccgacg aagcgccgct cgagacccag atagaaggca
     9841 catccggctt cagccaggag ttcgctgcta ccggaccgcg cgatagagag ggccggtcgc
     9901 tgcgcgactt cgatctcaag cgccgcatct tcaagtatcc gtgtagttac ttgatctact
     9961 ccgaggcttt cgatgcgctg ccagcgccag ccaaggaatt tatctacaag cgccttctcg
    10021 cggttctctc ggggcgcgac cagagtggcc gttacaaccg gctcacgccc ggcgagcggc
    10081 gagacattct ggagatcttg ctcgctacca agcctggtct gcccgacgaa tggaagcggc
    10141 tggggaccgc tcccgttgcg aaataattat ccccggctcg aagaaccaac cctatgtctt
    10201 gatgaaagga actactcgat gagaaaactg gtttgccttg gcattttgct caccttcggc
    10261 ccgctcgccc gcggcgaaag cgtgacgggt cggtgggacg cgactgtcac aatcaaggat
    10321 gccgagatcc cgttccgcat cgacttttcc ggcgagggca cgcagttcac cggcactgtc
    10381 tttaacggcg acttgccggt ttcgtcgacc agcggccggc tggagaacgg caagctggaa
    10441 gtaagtttcg gacactatct ctccaggctg gatgccaccg ttaaggatgg ccaaattgag
    10501 ggcaagctct ccggccggtt tgagcgcgat cgttatatca gctcgtatcc gtttcgtgcc
    10561 cagcgataca cgccgctggc tgcgacgtcg gaaaatgttc ccgagatcga cggcatctgg
    10621 gagattccat acaacagccc caagggggag aaatcgtggc atttcatcgt gcggcagaag
    10681 gggccggaag ttgcggcggc gattctgcgg gtcgacggcg acacgggtac cctcaccggg
    10741 acttacaagg acggcaggtt ctcggtgagc cacttctccg gctcccggcc gttacgtctg
    10801 gagatcacgc cgggccggaa taacacgctt gagatcgttc ccagcggggc ttacaccgcc
    10861 aaggagaagc tcgtcgccta tcggccggcc gatgcgcggg ccaaggggct gcccgagccg
    10921 accgatcccg acacgcacac tcgtgtcaaa aaacctgccg acgtcttcaa attcagcttt
    10981 ccggacgtgg acggcaacgt cgtgtcgaac accgactcgc gattcgtcgg caacgtcatc
    11041 gtcgcggttg ttaccggcac gtggtgcccc aactgccacg acgaagccca gctcttagtc
    11101 gagctcgatc gcaaataccg cgaccgcgga ctgcgggttg tggccctcga cttcgaggag
    11161 cccgagcaac aggaggacgg gctgaagcga gcccgctctt tcatcaagaa atacggggtt
    11221 gagtacccct acttgatcgc gggagcaccg attgagatgt gggagaaggt gccgcaggct
    11281 gaaaacctca attcatggcc cacgaccttc ttcgtcggcc gcgacggccg cgtgaaactg
    11341 atccacgccg gatttgcagc cccggcgagc ggcgagtttc accggaaggt aagagaggag
    11401 tttatctcca ccatcgagcg cctgttggcg gagaacacga ccgccgccca gtgacacgcc
    11461 gcctgtgcgg ttttaaagtg cctgaccacc tctccgtcag gccagttgaa ggcgggactc
    11521 gggcaagccg gttgaagccc acgtttcagc cggcgtcatc tcaccccccc tttcccgagt
    11581 tctgccttca attcccaaaa aaccatcctc ccgagccggg attcgctcgg ggtgcgcgca
    11641 caattccaga cggaaaccgc ggcacggccg cacgaaggag cactcacatg agatctcgtc
    11701 ccatcgctgg cgcggccatc atcgcctcat tcattctgat cggttgctca aacgagcaag
    11761 aacttgccgt tgtcaatgag aggacggggt cgccgttgct cgacaaagga agcgtttcca
    11821 atcctctgcg catacagaaa ccggccgatg aagcgcgagc tcggcgcgcc ggcgacacat
    11881 ctcgattgcc acgatgaaac cggtggacca ctggtattcc ggtctcgtga tcgcagtaca
    11941 cgtcgttctc gttcgttcga ccattcaatc gacaccttcg agtcggccgc ctcgagcttt
    12001 catcctggcg gagtcaatgt cggtttcgcg gacggctcgg tgcggttcat caaggattcg
    12061 gtcagcacat tggcgttcag ccagtcaacg gggctaccga atgcgctcat tgccacgccc
    12121 aacggtaact gcaccaactc ggcacccatt tattcgttcc agcctggtac gcagctcggc
    12181 gtctggcaag cactgtcgac ccgcgcaggc ggggaggttg tgagcgcgga ctcgtactga
    12241 ccggattccg cccagactcg tttcgtcctt tctcaacgac cgttcagcga aaggaaagac
    12301 tcatgtcaag gttcgtgccg agccgtgcgc agtggcggct cgttctccac actctcgtga
    12361 tcgcggcgtc ggtgacggtg ctcgcttcgc gagccatcgc agaagacccg gccttggtcg
    12421 acatcgagca agacgccaaa cggatccatg aaaacgtcct cgtcctggat agccacgttg
    12481 acattccagt tgactacggc accggccgca acgatcccgg tatcgacggc tcgacccagg
    12541 ttgacctacc caaactcgag cgcggcggcg tggatgcggc agtgttcgcc gtcttcgcca
    12601 agcaagacaa acgcacgcct gggggcctct taaaggccaa agccgaagcg gatcgtaagc
    12661 tcaaggccat tctggatatt cccagacgct accctcatca ggctgcgctg gcgctgtccg
    12721 ctgcggatgt cgagcgcatt caccgcgaga agaaggttgc ggtgatcgtc gggttcttga
    12781 acgcggcgtc gctcggcaca gatctttccc tgatcgactc ttattatcga gccggaatcc
    12841 gaactttcgg cttcgttcac gcaggcaata acgatttcgc cgactcgtcg cggccgatcg
    12901 gcgatgacaa atacggtgag catggcggtc tctcttcact gggacgtgag gctgtcacgc
    12961 ggttgaaccg gctgggcgtg atcctcgacg tctcgcagct cacacccgag gcgctcttac
    13021 agagtgtaaa gctatcaaag gctcctgtcg tcgcgactca ttccgcaatc aaggcgctgg
    13081 tcgatagtcc ccgtaacctg agcgatacag agctcgacgc gatcaaggcg aacggtggcg
    13141 ttgtgcagat cgtcgcgttt agctattacc tgaaagcgcc acgattcgac ctcgccagca
    13201 agtacaaaga gctcgccgcg aggtacaaaa aggacacgcg cgagctcagc aaggaggagg
    13261 acgaagagct gcaccgcgag ctttatgccc tcgcgccacg agacgccacg gtcgatgacc
    13321 tggtgaatgc gatcacctat gccgttaaac ggatcggtat cgaccacgtc ggcatcagct
    13381 cagacttcaa ccatggcggc ggcatcgtcg gctggaagga cgagagcgag gcgctcaatg
    13441 tcaccgcgga gctgctccgt cggggctacg ccgaggacca gatcgccaag ttatggggtg
    13501 gcaattttct gcgggtcttc cgccaggtcg aaaacgtctc ggagcagctt cgcctggccg
    13561 attcacgacg gccctgattg tgctgaagag cacgcgcagg gacggtccac ctgtcggctt
    13621 cccgtgcgtg atccgaaatc ggcaagaagt gtaaatccgc cgccgcctga ccgcaccttc
    13681 gccttcccgg gacgggtgct tcagagcggc gcggacgatt aagacggcat tctttgtgtg
    13741 aaggaaggta gaagtgcaag caactgcgcc tcgaacctcg ttccgaatca tggctgacgg
    13801 tgaaatgatc gcggctgcgg actcgatcaa cgcgattgcc gattcggtcc ggtattttca
    13861 tcctgatatc tatcaagtcg aagaagtcat cgacggcaac cgaggcgccg gccgccgcgc
    13921 gttccggttt tggggatggg tcactcaccg cgacgatggc attgtcgcgg ttgaaccttg
    13981 cctgagcgtg ctggaaggtt gatcgggcgt tggttacgtg aattcactgc ccgccggtgg
    14041 tgtcgaagca atttggccat ccgcgactgt cctgccgcca cgcccagcga aaaggcttgc
    14101 aacatgccct cccgggacga atccaacccg cggcggtcct cgatcggaca aagcaacagc
    14161 gacacttcca gaccggccga ggcgcgcgaa ccggcaacac tgcccgctcg gccgcctcga
    14221 gttgggcggt cttaacttgg gcatcgactt gctccaagcg ctgcttgatc gacgtatgct
    14281 cgctcgttcc cggagtctct gcgacctggg cggccacagg cctcgtgcag cgtccatttc
    14341 cggtcggagg gcgtggactc gggcctgctc gccgacgaac gaccctgcac acgtttcttt
    14401 cttgtgtcag agccttataa tccagtacaa aaaaccactc ggagctttat cattccacgc
    14461 cgagacgaga agggtatttc cgcgcatgtt cgcactccgc acaagaacca gcttcctggc
    14521 cacggctttg gcctcgttga tcgcgtctgg cagtgccacg gccgcggatt ctccgcgtga
    14581 aagtgcggtc ctccccgccg atcaatccgc ggctctcgac cggatcaccg cggactcgct
    14641 ccgtggtcac ctctcattca tcgcttctga cctcctcgaa ggccgcgaga ctccgtcgcg
    14701 cgggctcgac ctcgccgccg aatacatcgc cgcacagttc cgccgcgccg gtctccaagc
    14761 ggtcggcgac gacgggtatt tccagacggc caactggacg atgctcgagc ccgacgccga
    14821 cggatttgct ttcgagatca aaggtccaca ggctccgccc attcaggtcg tcaaggagca
    14881 tgtgagtttg caacgcctac ggggtttcga tgtcgcaact gatggcatct tcaagatcga
    14941 cgcccaagac gccaagagcc tcgagacgct caagcctgcc gacgtcgagg ggcaggccgt
    15001 gtttgccgaa ctcgccggcg ctggttcgag agacagggcc cgtttcgctg cagtcacggc
    15061 gttcatgaat cggatgacgg ccctcaaggc caagctggtc gtgaacgtca gtagtcagcc
    15121 caccgccgct acgggcctgg cccctggccg tccggtcgac ccggaaaacc cgcggacgaa
    15181 tgacgccgcc cgcgccgagc gcccggggcc gccactagtc acagttcacg attccaaggt
    15241 ttttgcgctc ttccgatcac tccctcttgg tccgtcgaac aagactttgg tttcgatcca
    15301 tctgggtgaa ccggtgtcgc gaccgatcaa acttcgcaac gtcgtcggcc tgctgcgcgg
    15361 ctccgatccg gcgctcaagg atacctgcgt gatcgtcaca gcccactacg accacatagg
    15421 cagccgtggc gcggctggcg acggtagcga ccacattttt aatggcgcca atgacgatgg
    15481 cagcggcact gtctcggtcg tcgagatcgc gtcggccctc gccttactca agaatcggcc
    15541 gaaacggagc attcttttca tgaccttttt cggtgaagag aaaggattgc tcggttcgcg
    15601 ctactacggc cggcaccctg tcgttcccat cgaaaagacg gtggccgacg tcaacctcga
    15661 acaggtcggc cgcacagacg actccgaagg tccgcggatc ggcgctgcga cgatgaccgg
    15721 gcacggctac tcggatctta cgaacgtctt caccgccgcg ggacaggccg agggggtgcg
    15781 gttggaacgg catccgcaat tcagcgacgc ttttttcgca cgcagtgata accaggcgct
    15841 cgctgacctg ggtgtgcccg cgcacacaat ctctgttgcg tttatgttcc cggactacca
    15901 cggccttacc gaccactggc agaaaatcga ctacgcgaac atggccaaag tcgaccgaat
    15961 ggtcgcgcgc gggattctca tgctcgcaga caatccggtc gaaccaaag
//
LOCUS       DATAQL010001297         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5475383, whole genome shotgun sequence.
ACCESSION   DATAQL010001297 DATAQL010000000
VERSION     DATAQL010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5475383"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VHS97_22400"
     CDS             <1..210
                     /locus_tag="VHS97_22400"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3451022.1"
                     /translation="KFSYRKAADIEREIGELEHELRELEDLLGQPATWRDPVKAVGFQ
                     DRHTGLKNRLETLYQHWETAVEANW"
     gene            210..1073
                     /locus_tag="VHS97_22405"
     CDS             210..1073
                     /locus_tag="VHS97_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3451023.1"
                     /translation="MPDPKKVLTTVRRASELTRTTPGRSGGIVALETAAEVMVVGDLH
                     GNIAAFKKVLAIAALERNPGRHLILQELIHGPLMYPDDKGDRSHQLLDVFTALKCQYP
                     ERAHLILGNHELSELTGRSIGKDGEGLNAKFRRGVETAYGESAGEILESYKTLFASLP
                     LAVRTRNRVYVCHTIPDESDLGNLDLELLKADVWPEDAMKRRGTIYALTWGRDTAPET
                     ADRFSAMVDADLFVTGHQPCDQGFRQANHRQIIIDGTHRHASYCLFPADGPITIESLL
                     SCVHLIDAFAG"
     gene            1213..>2166
                     /locus_tag="VHS97_22410"
     CDS             1213..>2166
                     /locus_tag="VHS97_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451024.1"
                     /translation="MKHLPRRTIRVSALIVILMGWSSPSLSAHADDEAGGDAATIYTA
                     SDFTWLAQGVDPGFTGPATLRVWAPPRDNWRLTEADGVLTLILASKEGDPEPQWRTLG
                     NVTLSQRKPLKIVVARDSADGGDKGPETPQDHPQSKTAKAEAAKSKQPLPVPALLLVS
                     ARPDIDTRATHDIVRGRTDSIEPTNDQRRTRVRTNWQGADFQAPQNVAAWQDRKRHLR
                     EQMLVALGLWPMFPKTPLAPKISGKLNRDQYTIEKVVLETFPGFTLSGNLYRPINQRG
                     RAPAIMCPHGHWDDGRVNPEVQQRCIRWAKLGCVVFLYDMVG"
BASE COUNT          451 a          710 c          629 g          376 t
ORIGIN      
        1 aagttctcct accgcaaagc cgcggacatc gaacgcgaga ttggtgaact tgagcatgaa
       61 ttgcgcgaac tcgaagacct gctcggccag cccgcaacct ggcgcgatcc cgtcaaagcc
      121 gtagggtttc aggatcgcca tacggggctc aaaaaccggc tcgaaactct gtaccagcac
      181 tgggaaaccg cagtcgaggc aaattggtaa tgcctgatcc gaagaaggtt ttgacgactg
      241 tccgccgcgc ctccgagctg acgcgtacca cgcccgggcg ctcggggggg attgtcgcgc
      301 tagagacggc cgccgaagtg atggtcgtgg gggatctgca cggcaatatc gcggcgttca
      361 agaaggtgtt ggcgatcgcg gccctcgagc gtaatcccgg ccgacatctg atcctacaag
      421 agttgatcca cggcccgctc atgtacccgg acgacaaggg ggatcggtcg caccagctcc
      481 tcgacgtatt cacggcgctc aaatgccagt atccggaacg agcccacctg atcctgggta
      541 atcacgagct gtcggagctg accggccgat ccattggcaa agacggcgag gggctcaacg
      601 ccaagtttcg gcgaggtgtc gagactgcct acggcgaatc ggcgggcgag attctcgagt
      661 catacaagac cctgttcgca tcgctccccc tggcggtgcg gacacgtaat cgggtctacg
      721 tctgccacac gatccccgac gagagcgacc tcggcaacct cgatctcgag ctgctcaagg
      781 ccgacgtttg gccggaggac gccatgaagc ggcgcgggac gatctacgcc ctcacgtggg
      841 gccgcgacac agcgcccgag acggccgacc ggttctccgc catggtcgac gccgacttgt
      901 tcgtcactgg tcatcagcca tgcgaccagg gattccgcca ggccaaccac cgccagatca
      961 tcatcgacgg cacccaccgc cacgcctcct attgcctctt cccggctgac ggccccatca
     1021 ccatcgaatc gctcctctct tgcgtccact tgattgacgc tttcgcgggg taaagaaccg
     1081 gcggaaccgg cgagtcgatc gacgtctccc cgcttgagtc aaccgcagtc tcggttagga
     1141 tgatgtctga gagccttata aagtacgtcc ctccctcgtt gcgccgcggc ttccaacaaa
     1201 cagggattca ccgtgaagca tctcccgcgc cggaccatcc gcgtatctgc gttgatcgtc
     1261 attctcatgg gctggagctc gccatctttg tccgcgcacg ccgacgatga agcgggcggt
     1321 gacgccgcaa cgatctacac tgcgagtgac ttcacctggc tggcacaggg agtggaccct
     1381 gggttcaccg gcccggccac tctccgggtc tgggctcctc cacgcgacaa ttggcgcctc
     1441 accgaggcag acggcgtcct cacgctcatc ctcgccagta aagagggcga cccagagccg
     1501 caatggcgga cgcttggcaa cgtgacactg tcgcaaagga agccgctcaa aatcgtcgtc
     1561 gctcgcgata gcgcggacgg cggggacaag gggccggaaa ctcctcagga tcacccgcaa
     1621 tcaaaaaccg ccaaggccga agcggccaag tcgaaacagc ccttgccggt tcccgcgctc
     1681 ttgttggtct ctgcccgccc ggatatcgat actcgagcca cgcatgacat tgtcagaggt
     1741 cgcaccgact cgatcgagcc aacaaacgac cagcgccgga cacgcgtgcg aaccaactgg
     1801 cagggggcgg attttcaggc cccccaaaat gtcgcggcct ggcaagaccg caagcgccac
     1861 ctccgcgagc aaatgctggt cgccctgggg ctctggccga tgtttcccaa gacgccactc
     1921 gctccgaaaa tcagcggaaa actcaatcgc gatcaataca caatcgaaaa ggtcgttctc
     1981 gagacgtttc ccggcttcac cctgagcgga aatctatacc ggccaataaa tcaacgcggc
     2041 cgcgcgccgg cgatcatgtg ccctcacggc cattgggatg atggccgggt caatcccgaa
     2101 gttcaacagc gctgcatacg ctgggccaag ctaggctgcg ttgtgtttct gtacgacatg
     2161 gtcggc
//
LOCUS       DATAQL010001298         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4405153, whole genome shotgun sequence.
ACCESSION   DATAQL010001298 DATAQL010000000
VERSION     DATAQL010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4405153"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..791
                     /locus_tag="VHS97_22415"
     CDS             297..791
                     /locus_tag="VHS97_22415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3451025.1"
                     /translation="MSKPRVYFDISIDGKPAGRITMELYNDVVPKTVDNFRALCTHEH
                     GFGYKKSQFHRVIPQFMLQGGDFTNHNGTGGKSIYGAKFNDENFSLKHNKPFLLSMAN
                     SGPNTNGSQFFITTVVTSWLDGKHVVFGEVTDGSDVVKKIESLGSSSGKPQAKIVIDD
                     CGEL"
     gene            938..2026
                     /locus_tag="VHS97_22420"
     CDS             938..2026
                     /locus_tag="VHS97_22420"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX3451026.1"
                     /translation="MQDEVVARWADLLVDYCLKVEQGETIALSSEILARPLVEACYKA
                     IVLRGAHPLVRLELPGLHEFFLQEATEAQLTHVPPPALFEAQTVNARIRIAAESDTRS
                     MGQINPARQALFERARDPIRQAARRARWVLTQYPTTAYAADARMPLSEYEAFVARALF
                     LDRPDPQGSWQELGRRQAGLVEYMAGVKSVRIEADDTDITLSVAGRTWINSDGKRNMP
                     SGEIFTGPVEESATGRLRCGFPVCRDGRELVGIRLEFVDGKVVQAKAEQGEDYLLAMV
                     DIDAGARRLGELGLGLNSGIDRFTGSILYDEKIGGTVHLALGQSYPETGGVNNSALHW
                     DLIVDTRTQGRITADGRLVMENGQWLVG"
     gene            2070..3416
                     /locus_tag="VHS97_22425"
     CDS             2070..3416
                     /locus_tag="VHS97_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451027.1"
                     /translation="MQRGSGSFLSTLYVGLIAAVLCDFAGARDARAQAGADAALRDRV
                     LQLVDRLDTEKVEAREAATTSLIKLGPTILSLLPDPATVTSTERKNRLVRIRAALAEK
                     AAELNTGASRVTIIGKGIRLSEALQQLQRQTGNAVTDIREQLGAEVTNPALDLDVRDK
                     SFFEAIDEIAKLADVSTNYSTGDGSVGIMAGASATPAPGMPANKGTPLIQYSGPFRIE
                     LQQLNLSRDFRAGTAVANIRLEAAWEPRLRPMLLKLKAEELKVADDRKKVVKPQIERE
                     AEEVVLRPENPAAEINLNLDAPERDAKKLGSLLVKAEVTIPAGIKTFKFPSLAQKGVT
                     SKQEDVSVTLEDIEVDEQVWKVNVTLVYPGEGPAFESYRQGLFNNRLWLQRADGSRFE
                     HNGGFSNTGSDGGKLGFEYLFVDAPGKPEDYQLIYETPSKVITIPLEFEFKDVVLP"
BASE COUNT          735 a         1033 c         1050 g          607 t
ORIGIN      
        1 ctcttccacg atgggactgg tcgccccatg gccattacaa tggcgagttg cgccgcaatt
       61 tcattgatga tcacactagc aagcgagcga atgaacaaag ttcacttgat gagtgccgcg
      121 caatcgtgac ggcaaaccag ggccgtgccc gtcgcaggcg ggaacttgag gtttggggga
      181 caagtcgtta aggtctaatc agacatttct gcacgcgggg aaccgctggg gacgagagcg
      241 agcaatgtgc ggttccgttc aatcacgagt caggaacagg atcgaggatc acagacatgt
      301 ccaaaccacg cgtttacttt gacatcagta tcgacggcaa gcccgccggg cgcatcacca
      361 tggaattgta caatgacgtc gttcccaaga ccgtcgataa cttccgagcc ttgtgcactc
      421 acgagcacgg ctttgggtac aaaaaatcac agttccaccg cgtgattcct caattcatgc
      481 tccagggggg cgatttcacg aatcacaacg gcaccggggg caagtcgatc tacggggcta
      541 agttcaacga cgaaaacttc tctctcaagc acaacaagcc cttcctgctc tcgatggcaa
      601 actccgggcc caacaccaac gggtcgcagt ttttcataac gacggtcgtg acctcgtggc
      661 tcgatggcaa gcatgttgtc ttcggcgagg tgaccgatgg cagtgacgtc gtgaagaaaa
      721 tcgagagtct gggctcgagc agcgggaaac cgcaggccaa gatcgtcatc gacgattgcg
      781 gcgaactttg agtgcgtgcg gaagagacat gcgaccgcgc cgcaggagcg aggtacgtgc
      841 tcaacgcgcc gcgacccatg ttgcagtggc gagggcgatg cgataagctt ttttcagggc
      901 tacgacactt gtctctcgga agcggggagc ggaacgcttg caagacgaag tggtcgcgcg
      961 ctgggccgat ctgctggtgg attactgctt gaaagtcgag caaggggaga cgatcgccct
     1021 aagctcggaa atcctggcgc gtccgctcgt ggaggcctgc tacaaggcga tcgtcttgcg
     1081 cggcgcgcac ccgctggtgc ggctcgagct gcccggtctt cacgagtttt tcctgcagga
     1141 ggcgaccgag gcccaactga cgcacgtacc tccgccggct ttattcgagg cgcagacggt
     1201 gaatgcgcgc attcggatcg ccgcggagag tgacacgagg tcgatgggtc agatcaatcc
     1261 ggcacgacag gcgctgttcg agcgagcgcg agacccgatc cgccaggcgg cgcgccgcgc
     1321 tcggtgggta ttgactcagt atccgaccac cgcctacgcg gcggacgccc gcatgcctct
     1381 ttcagaatac gaggcgtttg tcgcccgagc gctatttctc gataggcccg acccacaagg
     1441 gtcgtggcaa gagctagggc gtcgacaggc gggcctggtc gaatacatgg caggggtaaa
     1501 gtccgtccgt atcgaggccg acgacaccga catcaccctc tcggtcgccg ggcgcacctg
     1561 gatcaattcc gacggcaaac gtaacatgcc ttccggtgaa attttcacgg ggcccgtcga
     1621 agagtccgct accggccggc tgcgctgcgg gtttcccgta tgccgcgacg ggcgcgagct
     1681 ggtcgggatc cgccttgagt tcgtggacgg aaaggtcgtc caagcaaaag ccgagcaagg
     1741 tgaagactac ctgctggcga tggtcgacat cgacgccggc gcgcggcggc ttggcgagct
     1801 ggggctcggg ctcaacagcg gcatcgatcg ctttaccggc agcatcctct acgatgagaa
     1861 aattggcggg acggtccatc tggcactcgg gcaaagctac cccgagaccg gtggcgttaa
     1921 caattccgcc ttacactggg acttgatcgt cgatacgcga actcaaggcc ggatcacggc
     1981 cgacggccgg ctcgtcatgg aaaacggaca atggctcgtg ggatgatcgg ccatgtgccc
     2041 agccaatctc tgaaaacgga gaatccgaca tgcagcgcgg ctcaggctca tttctttcga
     2101 ccctgtacgt gggattgatc gcggcggttc tctgcgactt cgccggcgcg cgcgacgcac
     2161 gcgctcaggc cggggcggat gccgccttgc gtgatcgagt gctccagctc gtcgaccgtc
     2221 tggataccga aaaagtcgag gcacgcgagg ccgcgacgac gagcctgatc aagctcgggc
     2281 ccaccatcct gtccctgctt cctgatccgg cgacggtcac gagcaccgag cgcaaaaacc
     2341 gcctcgtgcg catccgcgcg gccctcgccg aaaaagcggc cgagctcaac accggcgcca
     2401 gtcgcgtcac gattatcggc aaggggatcc gcctgagcga agcgctccag cagcttcaga
     2461 gacagacggg caacgcggtc accgacatac gtgagcaact cggcgccgaa gtcaccaatc
     2521 ccgcgctcga cctcgacgtg cgagacaagt cattcttcga agcaatcgat gaaatcgcga
     2581 agctggccga cgtgtcgacg aactattcga cgggtgacgg ctcggtcggc atcatggccg
     2641 gcgcgtcggc gacgcccgcc cccggcatgc ctgccaacaa gggaaccccg ctgattcagt
     2701 acagcggacc gttccggatc gagctgcagc agttgaacct ttcgcgtgat ttccgggccg
     2761 ggacggcagt cgccaacatc cgcctcgagg ccgcctggga acctcgactc cgacccatgc
     2821 tcttgaagct caaagccgag gaactgaagg tcgccgacga ccgcaagaaa gtggtgaagc
     2881 cgcagatcga aagggaagcc gaagaagtcg tgctccggcc agagaatccc gccgccgaga
     2941 ttaacttgaa cctcgacgct cccgaacgtg acgccaaaaa actcggctcg ctcttggtca
     3001 aggccgaggt gacgatcccg gccggcatca agacattcaa gttccccagc ctcgcccaga
     3061 agggcgttac ctccaagcaa gaagacgtga gcgtgaccct cgaggacatc gaagttgatg
     3121 aacaggtctg gaaggtcaat gtcaccctgg tctatccggg tgaaggtccg gcattcgaga
     3181 gctatcgcca gggcctcttc aacaaccggc tctggctcca gcgcgccgat ggctcgcggt
     3241 ttgagcataa cggcggtttt tccaataccg gttccgacgg cggcaagctc ggctttgaat
     3301 acctcttcgt cgacgcgcca ggcaaaccgg aagattacca gctcatctac gaaacaccga
     3361 gcaaggtgat cacgattccg ctcgagttcg agttcaagga tgtcgttctg ccgtgatcgc
     3421 ggttg
//
LOCUS       DATAQL010001299         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1964020, whole genome shotgun sequence.
ACCESSION   DATAQL010001299 DATAQL010000000
VERSION     DATAQL010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964020"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..316
                     /locus_tag="VHS97_22430"
     CDS             170..316
                     /locus_tag="VHS97_22430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451028.1"
                     /translation="MAHQLRSNPNGPPQSLRKIAADLAERGYVTPSGRPYSTSAITAM
                     LGKT"
     gene            complement(604..1110)
                     /locus_tag="VHS97_22435"
     CDS             complement(604..1110)
                     /locus_tag="VHS97_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451029.1"
                     /translation="MLRMVLLGLLIPLGVGVLSAMELRTPPRSAVAIVQPPAEPMADI
                     SDSHEALAKGDRLEVVVVHSETPEQAVVRSKAPEQAVLIDEGIALPENNSVGSSEPPR
                     PIARHRHNSKSKKVTTAARPRSEPKATIIERRAISQRSKSASDTEPCRLKEFGGLRKA
                     LNSDDCEI"
BASE COUNT          283 a          458 c          459 g          340 t
ORIGIN      
        1 taacacccgc tttggccgca gttggagaga gtcgtatcga ggttggcctg tcctacggcc
       61 ggccaattgg ggcactctcc ggagccgacg ccgccgacct tactcgcgaa ctcgcgaaac
      121 aaggcatcgg ttttcgcccg agcggttagg tgggcaagag ctagtcgcca tggcgcacca
      181 attgcgcagc aatccaaatg gcccgcctca atcgcttcgc aagatcgctg ccgatctggc
      241 cgagcgtggt tacgtcacgc cgagcggtcg gccctattca acttcggcaa tcactgcgat
      301 gctcggtaag acgtgaggcg ctgatgattt caggttggcc ccggaagagt tccggtggcc
      361 ggcgtcgcgt gccctccctt cgcggagata tggggacgca ccaacgataa gggtcgcgcg
      421 caaacggcgg ggcggccggt gtcgtaggtg tttcctgttg gcccatcggc gaactgccgg
      481 catgtctcgc tgaggtccgc ttagcggggt atagcggctg agatttgctc atgttgagtt
      541 cttcgcatct tgacccacag cggaccacga gcacggattg ttcgacttcg attcgtaggg
      601 ctctcaaatt tcgcagtcgt ctgaattcaa agccttgcgc aagccaccaa actccttgag
      661 ccgacagggc tcggtatcgc tggcggactt tgaacgctgg gaaatggcac gtcgctcgat
      721 aatggttgcc tttggctccg accggggacg cgcagcggtc gtgaccttct tcgatttgga
      781 attgtgccga tgacgggcga tcggcctcgg aggctccgaa gagccgacgc tgttgttttc
      841 cggcagagca atgccttcgt cgatcaaaac agcctgttct ggcgccttgc tgcgcacaac
      901 ggcctgttct ggcgtctcgc tgtgcacaac gacaacttca aggcgatcac cctttgccaa
      961 cgcctcgtgt gaatcggaaa tgtctgccat tggctcggca ggcggctgga cgatcgctac
     1021 tgcactgcgc ggcggagttc tgagctccat tgctgacagg actcctacac ccaacgggat
     1081 gagcaagccc aacaacacca ttctcagcat gatgcgcctc tatcctgagt tagtgccggt
     1141 gaagattctc cgctccaatc aggcaatttg tcggcggctg ccggcccgtg tacggatcat
     1201 ttaccgacag ggttaacgtc ggcgcccccg acacttgcct cgatgtcggc tatcagaggt
     1261 aaccccgaca tggtcgccac gggacgaaat cgacgttcat gacccgaatg tatggtccgg
     1321 ccgtgcgttg caagtggttt cgccgagctg gcggtgagcg gtcttgcatc aatgtatccg
     1381 gcctttgatt ggagcatgtc tccgggccat catggatatc agcgcgcgtg cgttctcatt
     1441 accggatagg cctcgattgg accatttggg tcaccagtgt tcgcatgcgc cggtaagacc
     1501 gacctccatc tcgtctcatc ctctcgcaga cctcggctgg
//
LOCUS       DATAQL010001300         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4405187, whole genome shotgun sequence.
ACCESSION   DATAQL010001300 DATAQL010000000
VERSION     DATAQL010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4405187"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /locus_tag="VHS97_22440"
     CDS             complement(<1..2177)
                     /locus_tag="VHS97_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3451030.1"
                     /translation="MPRRPTVAWIVGLLLLVAGSARGQGPNRSSAKFYPDSSDTAEAL
                     LRNAASHARDRQWSAAIEIYQRVIDQFGDKVARLPKDDPAADQDGSFVLYVDDRHFCH
                     GAIAHFPPEARAIYRNRVDAIAERWFRQGASQRDAAQLRRVVDQAFCSSWGDDALELL
                     GDLAFQDGRFGEALAMYRRLVPDRALDTSLLVHPDPTVDLAKVEAKKLICRAAAGDDV
                     PAQAELGAFARRHPAAQGALAGRTGVYATIVAAAFESDHLARPFQPDSRWPTFAGSLT
                     RSKVVPGPVDVGSTQWRVELEKVWVNRQPTFGGPRGMTGGAQNTPQERLLAFHPIVLG
                     DQVIVCDGARILAYNLNDRPADSDQGMPRPVEPAWKHDPENGAQVPQAKPMQVGIPRY
                     TLTAVGNRIYARMGALSAAFLGGMGGRRGTSSIIALDWNTQGKKLWEQKSTSLSLPDR
                     PPDRNGSNSTVSFEGTPVANTRNVYVAVTDRREQTATYIACFDADTGASRWIRYLGAA
                     SPDVNNMFGMGMPMQFWMTAPGDFNHRLLTLDGPALYYQTNLGAVVALEAETGATLWV
                     ATYPRQEPNRLGSGSGSERDLNPAIVHDSRVFVAPSDADSIFAFDAGSGRLLWRSDPI
                     ADDVKLTHLLGVAKDRLVATGNRVLLFDVKTGKLLHAWPDSPKSLEGYGRGLLAGDLI
                     YWPTSGEIEVLDQRTALRAEPPIKLEATYHTKGGNLVAGDGYL"
BASE COUNT          435 a          738 c          687 g          417 t
ORIGIN      
        1 atcaggtagc cgtcccccgc gaccaggttg ccccccttgg tatgataggt cgcctccaac
       61 ttgatcggtg gctcggcacg caacgccgtc cgttgatcga ggacctcgat ctcgcccgag
      121 gtcggccagt aaatcaagtc accggccaag aggccgcggc cgtacccttc cagcgacttg
      181 ggggaatcgg gccaggcatg gagaagcttg ccagtcttga cgtcaaagag aagaacgcgg
      241 ttgccggtag ccaccagccg atctttggcc acccccagca agtgggtcag cttgacgtca
      301 tcggcgatcg ggtcgcttct ccatagcaag cgtccgctgc cggcgtcaaa tgcgaagatc
      361 gagtcggcat cgctgggggc cacgaagacc ctggaatcgt gtacgatcgc cggattcaaa
      421 tcgcgctcgc tgccgctacc actaccaaga cgattgggct cctggcgggg gtaggtcgcc
      481 acccacaacg tcgcgcccgt ctccgcctcg agtgccacga cagcgcccaa attggtctgg
      541 taatagagtg cgggtccatc gagcgtcagc aatcgatgat tgaagtcgcc cggcgcggtc
      601 atccagaatt gcatcggcat tcccatgccg aacatgttgt tgacatccgg cgacgccgca
      661 cccagatagc ggatccaccg gctggcgccc gtgtcggcat cgaagcacgc gatgtacgtc
      721 gcggtctgct cgcggcgatc ggtaacggcg acgtagacgt tgcgcgtgtt ggcaaccggc
      781 gtcccctcaa agctgactgt gctattgctg ccgttgcgat cagggggccg atcgggaagg
      841 gaaagagacg ttgatttctg ttcccagagt ttcttgccct gggtattcca gtcgagcgcg
      901 atgatcgagc tggtaccgcg ccgtcctccc atgccgccca gaaaggccgc gctcaacgcc
      961 cccatccggg catagatccg atttcccacg gccgtgagcg tataccgggg aatccccact
     1021 tgcatcggct ttgcctgcgg tacctgggct ccattctccg ggtcgtgttt ccaggcaggc
     1081 tcgaccggcc gcggcatgcc ctggtcggaa tcggccgggc gatcgttcaa gttgtatgcc
     1141 aggatccgag cgccgtcgca cacaatcacc tggtccccga gcacgatcgg atggaaggcc
     1201 aagagccgct cttgtggggt attttgcgct cccccggtca tgcctcgagg tccgccgaac
     1261 gtcggctgac gattcaccca caccttttcg agttccacgc gccactgggt cgagcccaca
     1321 tcgaccgggc caggaacaac cttcgaccgt gtcagcgagc cggcgaacgt cggccagcgg
     1381 ctgtcgggct gaaacgggcg tgccagatga tccgactcga acgctgcggc gacgatcgtt
     1441 gcatagactc ccgtgcgacc ggcgagtgca ccttgcgcgg cgggatgacg acgggcgaac
     1501 gcgcccaact ccgcctgggc cggtacatcg tctccagcgg ccgcgcgaca gatcagtttc
     1561 ttggcctcaa ccttcgccag gtcgaccgtt gggtcgggat gaaccagcaa actggtatca
     1621 agtgcgcgat ccggcaccag acgccgatac attgccaagg cttcgccaaa gcggccgtcc
     1681 tgaaacgcga gatcacccaa tagctcgagc gcgtcgtcgc cccacgaact acaaaatgcc
     1741 tgatcgacga ctcggcggag ttgtgcagca tcccgctggc tggctccctg gcggaaccag
     1801 cgctcagcga tagcatcgac ccggttgcga taaatcgcgc gcgcctctgg aggaaaatgg
     1861 gcaatcgcgc catggcaaaa gtggcggtcg tccacgtaca ggacgaaact accatcttga
     1921 tcagctgccg gatcgtcctt gggcaaccgg gccaccttgt cgccaaactg gtcgatcacg
     1981 cgctgataga tctcgatcgc cgccgaccat tgacgatcgc gtgcatggct cgccgcgttg
     2041 cgcagcaatg cttcggccgt gtcgctcgaa tcgggataaa acttggcgga gcttcggttt
     2101 ggtccctgac cgcgggctga tccggcgacc aacaacagca gtccgacgat ccaggctacg
     2161 gtgggacggc gaggcatcgt ggttcgatct gtcagccacg atcgcgaacc gcccatcacg
     2221 tgaccctctc ctacgacgtc gcccatcctg acgatattgg aaactagatc atcaggt
//
LOCUS       DATAQL010001301         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1202293, whole genome shotgun sequence.
ACCESSION   DATAQL010001301 DATAQL010000000
VERSION     DATAQL010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202293"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VHS97_22445"
     CDS             complement(<1..1529)
                     /locus_tag="VHS97_22445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3451031.1"
                     /translation="MIPDNSDRQTPTRPSRDGVGGAQDIEETGSYPPSASWWPTAPVA
                     DGPSTSHERFDFLSSPRADGEFGWLAHYRVLRLLGQGGMGLVFLAEDSLLARHVALKV
                     IRPEIADAPGIALRFTREARATAAIKHDHIVTIYQVGQENGVLFLAMELLTGLSLAQW
                     LDRGQIPSVELVLRIGREIAAGLSAAHRHGLIHRDIKPANVWLEAPSGRVKILDFGMA
                     RSVREDVEITRSGVVMGTPAYMAPEQARGEPANAGSDLFSLGCVIYRLCTCRLPFEGE
                     SVMAVLTALSTTTPRRPRDWRNELPPALDELVSRLLAKDPADRPESAEVVVKEIRTIE
                     RALFAERQKAERLAATPFSADVEFIKPNPTQAAEKPSLPQAAARPRNRIRTIGIGAAV
                     LSALMVAAVVGFVFAPARTSQVAMVAAGPTSAPANDDREVLRPFTTPKTDGVRTAQPV
                     ASPDRLSLRVDRGAASEPSPLTGLDVLQSKAAAEPALTLAPEDSQHVDPSAELVAPSV
                     R"
     gene            complement(2220..>2490)
                     /locus_tag="VHS97_22450"
     CDS             complement(2220..>2490)
                     /locus_tag="VHS97_22450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451032.1"
                     /translation="RERVSRPVHAAPPEDAPLPSIEVIERGHILTILGQTHWVIEGPR
                     GAAQIQGLHSQTSFAVAGQTRLRPNTLAYPSDSKANHNGGARLAG"
BASE COUNT          432 a          789 c          820 g          449 t
ORIGIN      
        1 tttctcacgc tcggcgcgac tagctcggcc gacggatcta cgtgttggga atcctccggt
       61 gcgagagtta gggccggttc ggccgcggct ttggattgca gcacatcgag accggtgaga
      121 gggctcggct ccgatgcggc ccctcgatca acccgcagac tgagacgatc ggggctggca
      181 accggttgag ccgtccgcac tccgtcggtc ttcggagtcg tgaatggccg aagcacctcg
      241 cggtcgtcat tggccggggc tgacgtcggc ccagcggcga ccatcgcgac ctggctcgta
      301 cgcgcagggg cgaagacaaa gccgacgact gccgccacca tgagcgccga caacactgcc
      361 gcgccgatcc caatcgtacg aatgcggttt cgcggccgcg ccgccgcttg tggcaggctc
      421 ggcttctcgg cggcttgggt cgggtttggt tttatgaatt ccacatccgc cgagaagggc
      481 gtggccgcca aacgttcggc cttttgccgc tcggcaaaga gcgcacgttc gatggtcctg
      541 atttccttga ccacgacctc ggccgactcc gggcggtccg ccgggtcctt ggcaagcagg
      601 cgcgatacca gttcgtccaa ggccggcggc agttcgtttc gccagtcccg aggccggcga
      661 ggcgtggtgg tcgacagggc ggtcagcacc gccatgacgg actctccctc gaaaggcaac
      721 cggcaggtac agagccggta gatcacgcag ccgaggctga acagatccga gccggcgttc
      781 gccggttcac cgcgggcttg ctccggagcc atgtaagccg gcgtgcccat gaccactccc
      841 gaacgagtga tctcgacgtc ctcgcgtacc gaccgcgcca tgccgaagtc aaggatcttc
      901 acgcggcccg agggcgcctc gagccagacg ttggccggtt tgatgtcgcg gtggatcagg
      961 ccgtgccggt gggctgcgga cagcccggct gcgatctcgc ggccgatccg caggacaagc
     1021 tctacggacg gaatctggcc tcggtccaac cattgggcta gagacagtcc tgtcaagagc
     1081 tccatggcca ggaacaggac accgttttcc tgcccgacct ggtagatggt gacgatgtgg
     1141 tcgtgcttga tcgcggcagt cgcccgagcc tcgcgcgtga accgtagcgc gatcccgggc
     1201 gcgtcggcga tctcgggccg gatgactttc aaggcgacgt ggcgggcaag cagactgtcc
     1261 tcggccagga agaccaggcc catgccccct tggcccaaga gcctgagaac gcgatagtgg
     1321 gccagccagc cgaactctcc gtcggcccga ggcgagctca gaaaatcgaa gcgttcgtgg
     1381 ctggtcgacg gaccgtctgc cacgggtgca gtgggccacc agctagccga cggaggatag
     1441 gagccagtct cctcaatgtc ctgggcccct cccacgccgt ctcgcgacgg acgggttggt
     1501 gtttgtcggt cagagttgtc ggggatcatg gctcactccg cggtcgacac gaagtaatta
     1561 tcgtaaaggc aagttagatt actggttatg ttcgcccagg gacttgacca ccctacggta
     1621 ggtggtctcg cgatcgcggt aggcccgggc gcagtcgttc agctgccgct ccttgtattt
     1681 gtcgcattcg gctccacaat cctgacaagc tagcgcgcac ggcggtcaca gtcaaagccc
     1741 ccagcatttc tcaacggatc gttggcattc tcccagtgcg tattggcttc cacatcaaca
     1801 acgcctgttg cgcggtacga gcccgtgcga cgcgtcagac tcgccgtcgc tatggcgaat
     1861 cgcacggctt gtgccaggcc gacgcacatg gggaatattg ctcgcaaacc caaaccgacg
     1921 atcaacctaa ggcgacggcg ttcatccagg cgcaagactg gctcgcgaaa gcgtaggatt
     1981 ggcatcacga aatgtcgtgg ctggatacga cggctcgtgg gccaagcgtg tggcgcccag
     2041 cttctttggc cagaccactc gtgtttgcgc ggtcgtccgc gctagagcag gggccgctct
     2101 ggatggcgcg gcagctcgac gccgccaagc ccgctgacct cgcccaaact tctcggcaaa
     2161 ttggaccggc accgggtcgg acggcgggcg gacgacgatg tgacggtcat aaccgttcgt
     2221 cacccggcca gccgcgcgcc gccgttgtga ttcgctttag agtcggacgg gtacgccaag
     2281 gtgttcggtc tcaagcgcgt ttgaccggca actgcgaacg aggtttggga gtgtaatccc
     2341 tggatctggg ccgcgccgcg cggcccctcg atgacccagt gggtctgccc gaggatggtg
     2401 aggatatgac cccgctcgat cacttcgatg gaggggagtg gtgcatcctc cggtggggcg
     2461 gcgtgaacgg gccgcgacac ccgttcacgc
//
LOCUS       DATAQL010001302         4508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3798049, whole genome shotgun sequence.
ACCESSION   DATAQL010001302 DATAQL010000000
VERSION     DATAQL010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3798049"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..604
                     /gene="trxA"
                     /locus_tag="VHS97_22455"
     CDS             272..604
                     /gene="trxA"
                     /locus_tag="VHS97_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX3451033.1"
                     /translation="MADSVKEFTDGNWKTEVLESPVPVVVDFWAPWCGPCRMLAPTIE
                     KLAGEFEGKIKVGKMNTDENQDTPGGLRISAIPTVLVFQNGKEVDRLVGVNPENKFKQ
                     SLDKLGVS"
     gene            685..1386
                     /locus_tag="VHS97_22460"
     CDS             685..1386
                     /locus_tag="VHS97_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX3451034.1"
                     /translation="MFHPVVGVKICGLTCVEDVTACAAAGVDWVGLNFHPGSPRFVQP
                     DRAAEIIASLPASISAIGVFVDRPAAEVEDLSQRVGLKIIQLHGSEPPEYLLTLGQLK
                     VIRAFRVGGTSAWDGVSEYLAQAEKLERPPYAILIDSCVPGQPGGTGATVGEDVLDSM
                     PPLPRLILAGGLTPANVAVKVERFRPWMVDVASGVESAPGRKDPALIAAFVRAAKSAR
                     VAVPVPSPVAVPGPS"
     gene            1541..2230
                     /gene="rnc"
                     /locus_tag="VHS97_22465"
     CDS             1541..2230
                     /gene="rnc"
                     /locus_tag="VHS97_22465"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX3451035.1"
                     /translation="MAECQKILGYRFGNLILLKEALVHASGADHRLASNERLEFLGDA
                     ILGAIVCEQLFRDFPDYLEGDLTRIKSIVVSRRTCAKISAELGLEQFLKIGKGMGSHA
                     QTPPSVLADVFESLIGAIYLDGGIEEAREFIIRHIGPEIDAAVEDTGGVNYKSNLQQI
                     AQRDFGETPSYLLLDEKGPDHSKCFQISALIGKRRFASAWGPNKKEAEQRAALNALCQ
                     LAGEPLPFETE"
     gene            2241..3521
                     /locus_tag="VHS97_22470"
     CDS             2241..3521
                     /locus_tag="VHS97_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451036.1"
                     /translation="MNSSLVTDLGLGPEIWLFLTLLGCVTLFFKFSRIWSVRNLDLLL
                     LFALAPGMMLLVGKGEHNSWVAYLGLFIGSGLWLIRCGADLGLARRPLLEPNLNASGL
                     LCLSLGILGLLLAETVSLPVEDGAARNPAEPTGREDRSAPGPGENSATAKQLIKIAPL
                     PSALKQQRPLVIVSRVLASLAHLGLVLGLLGIGWRHFDRPLTGMAMVACYLLLPYTRM
                     ALVDSAQLIPSALIVAAVFWHLRPALAGALIGLAGGWMPACLGLIALWCGFFRGRGAI
                     KFVTVALAVVICCALFGRFGPALVGDWARGLGARSIAEVGLLPHFQPRSEGSFWVSID
                     SSFRLPVLIAYLALVILFSIWPADKNLAELISLSALLLVASQFWYLEKGGTLVMLYLP
                     LAIAMMFRPTLSIRRPLSPAHQRRAVRPSLYPSS"
     gene            3586..4275
                     /gene="deoC"
                     /locus_tag="VHS97_22475"
     CDS             3586..4275
                     /gene="deoC"
                     /locus_tag="VHS97_22475"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX3451037.1"
                     /translation="MSFQLTYEAIAKRIDHSLLGPTLGHAELEDGCKLAARYGVASVC
                     IKPYGVALAARILKGSGVEVGTTVGFPHGGHLTAVKVFEAERAMADGATELDMVINIG
                     QAKGGEWEAIASDIAAVTKACHDGGAIVKVIFENCYLTDDQKVRLCKICGEVGADYVK
                     TSTGYGTGGATVDDLRLMRRSAPPHVKLKAAGGVRTLDAMIEAAALGCDRIGASRTAE
                     ILDELKGRLGA"
BASE COUNT          802 a         1319 c         1417 g          970 t
ORIGIN      
        1 tggttgccac ccccccaccg gtcttgcggc cgcggccaga aaaaggtgtc aggacccttt
       61 tttgcaaaaa agggtcctga cacctttttc tggccgcggc cgcaagaccg gtgggggggt
      121 ggcaaccaat cgaccaacca ccagccgtcc ccacggttga ctcgcttccc gggcacctta
      181 taatgcatcg tcaaggggtc gctgcgatcg aactcccttg cgttaagctt tgaatatcac
      241 tggactttcg cagatcgacg aggcaattga aatggcggac agcgtgaagg agtttaccga
      301 cggtaactgg aagaccgagg ttctcgagtc gccggttccg gttgtggtcg atttctgggc
      361 accttggtgc gggccgtgcc ggatgctcgc cccgaccatc gaaaagctgg cgggcgagtt
      421 cgaagggaag atcaaggtcg gcaagatgaa caccgacgaa aaccaagata ctccaggcgg
      481 cttacgcatc tcggccattc ccaccgtctt ggtgtttcag aacggcaaag aggtcgaccg
      541 tttggtcggc gtgaatcccg agaacaagtt caaacaatca ctggataagc tcggggtgtc
      601 ctgagcctgt tcgccgggcc aaccggccgg cggactatcc agattcgagc atggcctggc
      661 cgtgcaattt tgaagacttt gctcgtgttt caccccgtcg tcggagtgaa gatctgcggc
      721 ctgacttgcg tcgaagatgt gacggcttgc gcggcggctg gtgtcgattg ggtcggactt
      781 aactttcacc ccggttcgcc gcgttttgtg caaccggatc gagccgcgga gatcatcgcc
      841 tcccttcctg cctcgatctc cgcgatcgga gtttttgtgg atcggccggc ggcggaggtg
      901 gaagatcttt cccagcgcgt gggactgaaa atcatccagt tgcatggaag cgaaccgcca
      961 gaatacctcc ttacgctcgg tcagctcaag gtcatccgag cgttccgtgt cggtgggacc
     1021 tcagcctggg atggcgtgag cgaatatctc gcgcaggccg agaaactgga gcgcccgccc
     1081 tacgcgatct tgatcgactc gtgtgtcccc gggcagccgg gcggcacagg cgcgactgtg
     1141 ggcgaggacg tcctcgattc catgcccccc ctaccccggc tcatcttggc cggcggcctg
     1201 accccagcaa acgtggccgt caaagtcgag cggtttcggc cctggatggt cgatgtcgcc
     1261 agtggtgtcg agtcggctcc gggtcgaaag gatccggcgc taatcgctgc cttcgttcgc
     1321 gcggccaaga gcgctcgtgt tgccgttcct gttccatctc ccgtcgcggt tcctggtcca
     1381 tcttgagtcg gctttgaatc tgagcggatc gcaccctcaa catgagattg ttgacaagcc
     1441 ctggcgcggt ttatagccta tcagttctga atcgcaactc cttctgcgcc atgttggtgc
     1501 gaccatgccg cttacgactc ctcgggacga ttccggaccg ttggccgaat gtcagaagat
     1561 tctcggctac cggtttggaa acctgatttt gctgaaggaa gcgctcgttc acgcttcggg
     1621 agccgaccac cgactggctt ccaacgagcg gctcgagttc ttgggggacg cgatcttggg
     1681 tgcgatcgtg tgcgaacagc tgtttcgcga ttttcccgac tatctcgagg gcgatctgac
     1741 gcggatcaag tcgatcgtgg tatccaggcg cacttgtgcg aagatttccg cggaactggg
     1801 actcgagcag tttctcaaga ttggtaaggg tatgggttca cacgcacaga cgcccccatc
     1861 ggtactggcg gacgtttttg agagtttaat cggagcgatt tatctcgatg gagggatcga
     1921 ggaggctcgc gagttcatca tcaggcacat cgggcccgaa attgacgccg cggtcgagga
     1981 caccggcggc gtgaattaca aaagcaatct ccagcagatc gcgcagcgcg attttggaga
     2041 aactccgagc tatctcctgc tggatgaaaa ggggccggat cactcgaagt gtttccagat
     2101 ttcggcgctg atcggcaagc gccgcttcgc ttcggcctgg ggcccgaata aaaaggaggc
     2161 tgagcaacgc gcggcgctca acgcgctttg ccagctcgcg ggcgaacctc tccctttcga
     2221 gaccgagtga cgaggcagcc gtgaacagct cgctggtgac cgatcttggc cttggccccg
     2281 aaatctggct cttcctgacg cttttgggct gcgtcactct ctttttcaag ttcagccgta
     2341 tctggagcgt ccgcaacctc gacctgctgc ttctctttgc gctggcgccg gggatgatgc
     2401 tcttggtggg caagggtgaa cataactctt gggtcgcgta tttggggcta ttcatcgggt
     2461 cgggtttgtg gttgatccga tgtggggcgg acctgggatt ggcgcgtcgt ccgctactgg
     2521 agcccaatct caatgcatcg gggttactgt gtctctcgct gggaatactc ggtctgctgt
     2581 tggcggagac ggtgagcctg ccggtcgagg acggagcagc gcgcaaccca gccgagccaa
     2641 ccgggcggga ggaccgatcg gcacccgggc cgggcgaaaa cagcgcgacc gccaagcaat
     2701 tgatcaagat cgctccgctg ccatccgcgc tcaagcaaca gcgtcctctg gtgatcgtgt
     2761 cgcgcgtgct ggccagtttg gcccacctgg ggttagtgct gggcttgttg gggatcggct
     2821 ggcggcattt cgatcggccg ctgaccggta tggcgatggt ggcgtgctac ctgttactcc
     2881 cctacacccg catggctctg gtagatagcg cccagctgat cccgtctgct ctcatcgtcg
     2941 ccgccgtatt ctggcacctg cgcccggcat tggccggagc attgattggg ctggcaggcg
     3001 gctggatgcc ggcgtgtcta ggattgatcg cactttggtg tgggtttttc aggggccgag
     3061 gcgcaatcaa atttgtaacg gtcgcgctgg cggtcgtcat ctgctgcgcg cttttcggcc
     3121 ggttcggccc ggcgcttgtg ggcgactggg cacgtggcct gggcgcacgc agcatcgccg
     3181 aggtcggtct cttgccccac tttcagccca ggtcggaggg cagcttttgg gtgagtatcg
     3241 actcgagctt ccggctgccc gtactgatcg cttacctcgc tctggtgatc cttttcagca
     3301 tctggccggc cgacaaaaac ctggccgagc tgatctcgct ctcggctctc ttgctcgtcg
     3361 ccagccagtt ctggtacctc gagaaggggg gcacattggt gatgctctat ctcccgctgg
     3421 cgatcgccat gatgttccgc ccaacccttt cgatccgcag gccgttatcg ccggcacatc
     3481 aacgccgggc ggtccggcct tcgctctatc cttcctcttg attcaaactc gcgttatctt
     3541 ttggtggcca tcgatcggcg actggcattg gagaaacgtt taaagatgag ctttcaactg
     3601 acctacgaag caattgccaa gcgcatcgac cactccctct tgggaccaac gctgggccac
     3661 gcggagcttg aagacggctg caagttggcc gcgcgttacg gcgtggccag cgtgtgcatc
     3721 aagccctacg gcgtggcgct ggccgcgcgg atcttgaagg gctccggggt tgaggtcggt
     3781 acgaccgtgg gctttcctca cggtggtcat ctgactgcgg tcaaggtctt tgaggcggag
     3841 cgggccatgg ccgacggggc gaccgagctc gacatggtta ttaacatcgg ccaggcaaag
     3901 ggcggcgagt gggaagccat cgcctccgac atcgccgccg tgaccaaagc ctgtcacgat
     3961 ggtggagcga tcgtgaaagt catctttgaa aactgttact tgaccgacga ccaaaaagtg
     4021 cggctctgca aaatttgcgg cgaggtcggc gccgactacg tcaagacatc gacgggctac
     4081 ggcaccggcg gcgccacagt ggacgacctg cgcctgatgc gccgttcggc cccaccgcat
     4141 gtcaagctca aggcggccgg cggcgtccgc acgcttgacg cgatgatcga ggccgcggcg
     4201 ctcggctgcg accgtattgg cgcctcgcga accgctgaaa tcctcgacga gctgaagggt
     4261 cggctgggag cctgaccgcc ggttggtgaa agtaagctgc ttttgcccat cggtgaaggt
     4321 caagccggaa gctcctcatc gagtagaagc gggtacgact tccgactcac gagctcaggc
     4381 ttgatgtgca gcttggttga cgatccgttc gacaccggcg ttgctgccga cgatgagcgt
     4441 gtcgcaaagg tcgagaaaga gcccggtctc aagcaccccg gcaatacact ggagctgctt
     4501 atcgagcg
//
LOCUS       DATAQL010001303         3624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2730359, whole genome shotgun sequence.
ACCESSION   DATAQL010001303 DATAQL010000000
VERSION     DATAQL010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2730359"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VHS97_22480"
     CDS             complement(<1..625)
                     /locus_tag="VHS97_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3451038.1"
                     /translation="MEHKKAKHVIIGAGAMGAAAAYHLARRGEPVVLLEQFALGHDRG
                     SSHGAARITRHSYADPTYARLMPAAFRAWRELEAEGGESLYIRTGGVSFSPAAVDYAA
                     RVAANLRELDVPYWHASGRAWNKRYPAFGLPADYDVVFEPDAGVLRASRAVALELELA
                     RVHGRDQTRVIEQTPVRHIDLEGEKPVVITESAEIVADRVIVAAGGWV"
     gene            complement(674..2044)
                     /gene="glnT"
                     /locus_tag="VHS97_22485"
     CDS             complement(674..2044)
                     /gene="glnT"
                     /locus_tag="VHS97_22485"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEX3451039.1"
                     /translation="MPNLEQQLVELESRFQDDRISYLLIQFVDIHGAPKVKLVPAGNL
                     RSVAEVGAGFAGGAVWGMGQGPHSHDLQARPDLDSYTVLPYEPGVARLAADLYVDGQP
                     HPFCPRVNLKRVLAAAAAQGYLFNVGIEPEFFLVTKNPDGTIRGWDPDQVDDLAKPCY
                     DFKGMSAALGFLRELNDGLNRLGWGVYQSDHEDANFQYEVNFRYAEALITADRLTFFR
                     MMTGQLARRIGAIATFMAKPFSNRTGSGAHMHYHLADSSSGENLFLDPADRRGLGLST
                     LAYHFLGGVLEHAPALCAVTSPTVNCYKRLQMGPALTGSLSGYTWTPAFISYGDNNRT
                     QMLRIPEGGHVEDRSVSSASNPYLAIAAYLAAGLDGIARRLDPGEPNLGNLYAADMET
                     MVRRGVRTLPQSLPEALACFERDGVVRESLGPIAQEFYRLKRDEWREYHAQVESWEIK
                     RYLTAL"
     gene            2208..3152
                     /locus_tag="VHS97_22490"
     CDS             2208..3152
                     /locus_tag="VHS97_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine phosphoribosylpyrophosphate
                     amidotransferase"
                     /protein_id="HEX3451040.1"
                     /translation="MCGIVAFWDKSGGHEAATGKVILSMLEALACRGPDSAGIALVGP
                     EPEPEMTGVWLIRITPPDTELLDRLTGVGRLVAPARGAAWERQGNTLRFAFQPAPGIT
                     PLDLERALGARRGGLEVLSLGERMDLVKQVGSPSLLEDLYGVSTWSGPLAIGHTRMST
                     ESKIDLSHSQPFWAHGVPDLATVHNGHVTNYQQLRRRYQQRGATFLTDNDSEVIGVYL
                     RERMLAGRSLAEAMADSIADLDGAYNYLVVSSQGLGIVRDRFGFKPLMLAETERFVAV
                     ATEEIALRRAISGDYTVSEPSPRSALFYPLAARVPAFA"
     gene            3167..>3624
                     /locus_tag="VHS97_22495"
     CDS             3167..>3624
                     /locus_tag="VHS97_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEX3451041.1"
                     /translation="MTLAPRFDGTTVDCGDLSVRAINQAIRSAQVEGATQIRLIRPGA
                     RHNLGVALPAGLFVTIEGSVGYYVAGLNDGAAVTVHGSAGWGAGESMRDGSVVIDGNA
                     GNAVAASIRAGTVVVRGDVSTRAGIAMKGGTVIVAGDAGPMAGFMMQKGVL"
BASE COUNT          671 a         1109 c         1180 g          664 t
ORIGIN      
        1 tgacccagcc gccggccgca acgattacgc ggtcggcgac aatttcggcg gactcggtga
       61 tgaccaccgg cttctccccc tcgaggtcga tatggcgcac cggggtttgt tcgatcaccc
      121 gagtctgatc gcggccgtgg acccgggcca gctcgagctc gagggcgaca gcacgtgaag
      181 cgcggagcac tcccgcgtcg ggctcgaaga ccacatcgta atccgcgggc aagccaaagg
      241 cgggataacg cttgttccag gcccggcccg atgcatgcca gtacggcaca tccagctcgc
      301 gcaggttggc ggcgacgcgg gcagcgtagt cgactgccgc cggcgaaaac gaaacgccgc
      361 cggttcggat gtaaagcgat tcaccccctt ccgcctcgag ctctctccag gcgcggaacg
      421 ccgcgggcat caatcgggcg tacgtgggat cggcatacga gtgccgcgtg atccgcgccg
      481 ccccgtgcga actgcctcga tcatgaccaa gcgcaaattg ctccaagagc acaaccggct
      541 caccacggcg agcgagatga taggctgccg ccgcgcccat cgctccggct ccgatgatca
      601 catgttttgc ttttttatgt tccataactc gcatccacga tttttacggc gaggccgttt
      661 attgcacacg tttttagagc gccgtcaggt aacgtttgat ctcccacgac tcgacctgag
      721 catgatattc gcgccactca tcccgcttga gacggtaaaa ctcctgggcg atcgggccca
      781 agctctcgcg aaccacgccg tcgcgctcga aacaggcgag ggcttccggc aggctttgcg
      841 ggagcgtgcg cacgccccgg cggaccatcg tctccatatc cgccgcgtag agattgccca
      901 gattcggttc gccggggtcg agccggcgag cgataccgtc taacccggcg gccaggtagg
      961 cggcaatggc cagatagggg ttgctcgcgc ttgaaaccga gcgatcctcg acgtgccccc
     1021 cctcggggat tcgcagcatc tgtgtccggt tgttgtcgcc ataagagatg aaggccggcg
     1081 tccaggtgta gcccgacagc gagccggtca gggctggtcc catctgcaat cgcttgtagc
     1141 agttgaccgt cggcgaggtg accgcgcata gggcgggcgc gtgctcgagc acccccccga
     1201 gaaagtggta tgccagggtc gaaagaccca ggccccgacg gtccgctggg tcaagaaaca
     1261 gattctcgcc ggacgacgag tcggcgagat ggtaatgcat gtgggctccc gagccggtgc
     1321 ggttcgagaa cggcttggcc atgaatgtcg cgatcgcccc gatccgccgg gcgagctggc
     1381 ctgtcatcat cctgaaaaaa gtcagccgat cggccgttat aagtgcctcg gcatagcgaa
     1441 agttgacttc atactggaaa ttggcgtctt cgtgatcgga ctgatagacg ccccagccga
     1501 gccgattgag gccgtcgttg agctcgcgca aaaaccctaa cgcggcagac atgcccttga
     1561 agtcgtaaca cggcttggcc aggtcgtcga cctggtcggg atcccagccg cggatcgtgc
     1621 cgtccgggtt tttggtcacc aagaaaaact ctggctcgat acccacgttg aaaagataac
     1681 cctgggctgc tgccgcggcg agcacacgtt tgagattgac gcgaggacag aatggatggg
     1741 gttggccgtc gacatagagg tcagccgcga gccgcgcgac cccgggctcg tacggcaaga
     1801 ccgtatagct gtcgagatca ggtcgggcct ggagatcgtg ggaatgcggc ccttgcccca
     1861 ttccccagac ggcccctcca gcgaaaccgg cgccgacttc cgcgaccgac cgcaggttgc
     1921 cagccgggac aagcttgacc ttgggcgcac cgtgaatatc gacgaactga attagcaaat
     1981 aagaaatgcg gtcatcttga aatcggctct cgagctcgac aagctgttgt tccagattgg
     2041 gcatgcgtac gctggtcctt tcttgggccg atgtcgactc gccacctgga aaatggcctg
     2101 gcgagcgtgc atccccgttt tgaccctcga ttgttggcaa aatgactcgt ccaagccaac
     2161 aaaattggag ccgtggcacc tgaaatcggc agggctgggg gagtgatatg tgtggtattg
     2221 tcgcgttttg ggacaaaagc ggcgggcacg aggcggccac cggtaaggtg atcctgtcga
     2281 tgctcgaagc cctcgcctgc cgcggccccg atagcgcggg aatagccctg gtcgggccgg
     2341 aacctgagcc ggaaatgacc ggggtttggc tgatccggat cacgccgccg gacacagagt
     2401 tgctggaccg gctgacgggg gtcggccggc tcgtggcgcc tgcacgaggg gccgcctggg
     2461 agcgccaggg caacaccctg cgctttgcct tccagccggc accgggaatc acgcccctgg
     2521 acctggaacg ggcactcggt gctcggaggg gcgggctgga ggtgctcagt ctgggcgagc
     2581 gaatggatct ggtcaagcaa gtcgggtccc cctcgttgct cgaggacctc tatggcgtgt
     2641 cgacctggag cggccccttg gctattggcc atacacgcat gtcgacggag agcaagatcg
     2701 acctctccca ctcgcagccg ttttgggcgc acggagttcc tgacctggcc accgtgcaca
     2761 acgggcacgt taccaactac cagcagttgc gccgccgcta ccaacagcgg ggtgcaacct
     2821 ttcttaccga taacgactcc gaggtgatcg gagtctatct tcgcgagcga atgctcgcgg
     2881 ggcgatcgct cgccgaagcc atggccgact cgatcgctga cctcgacggc gcgtataact
     2941 acttggtcgt ctcatcgcag ggactgggga tcgtccgcga ccgcttcgga ttcaagcccc
     3001 tgatgctggc cgagactgag cgtttcgtgg ccgtagccac cgaggagatc gctcttcgcc
     3061 gcgcgatctc gggagattat accgtgtctg agccgtcgcc aagatcggcc ttattttatc
     3121 ccttggccgc tcgcgtgcca gcgtttgctt gaggtgcgtt gttgcgatga cacttgctcc
     3181 gcgttttgac ggtacaacgg tggactgcgg cgacctgtcg gtccgggcaa tcaaccaggc
     3241 catacggtcc gcgcaggtgg agggagccac ccagattcgc ttgattcgtc caggtgctcg
     3301 gcacaacctg ggcgtggcgc tgccggccgg cctcttcgtg acgatagaag gaagcgtcgg
     3361 gtactacgtc gccggcttga acgatggcgc ggcggtcact gtgcacggta gcgcaggctg
     3421 gggagccggc gagagcatgc gggacggctc ggtcgtgatt gatggcaacg cgggcaacgc
     3481 ggtggccgcg tcgatccgcg cgggaacggt ggtcgtccga ggcgacgtgt caacgcgggc
     3541 cggcatcgca atgaaagggg ggacggtgat cgttgccggc gacgccggac ccatggccgg
     3601 cttcatgatg cagaagggag ttct
//
LOCUS       DATAQL010001304         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4255484, whole genome shotgun sequence.
ACCESSION   DATAQL010001304 DATAQL010000000
VERSION     DATAQL010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4255484"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..>2296)
                     /locus_tag="VHS97_22500"
     CDS             complement(238..>2296)
                     /locus_tag="VHS97_22500"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451042.1"
                     /translation="ESAMTLQDRRASERLHSRPCLLVSTWLLSLVSICLANPSAQAGS
                     DPTLQIEVDARDLPRRLLHARIVVPCQPGKLGLWYPKWIPGTHAPSGAIQDVAGLRVE
                     TPEGKPVPWRRNDIDVYRVECDVPDGVREIVARLDVICNGPAVEASGHLSYGNNSVGM
                     INWSTCLLYPDGPSCDQIRAHLSLRLPRDWRFATALATESPRDAQVPKPAGDAPVAFK
                     TVTVTDLVDNPLIAGEHLRAIPLNTGPYPPAFLDLVSESPSALRIGPNVVDMYSRMVR
                     EAGALFGACHYPDFHFLVTCSDDLGYHGLEHLACSINGVHERDLLDDSKRKGWVANLI
                     PHEYVHSWCGKFRRPAGMCTPDFQTPLKTRLLWVYEGLTEYLGEVLMVRAGLVDSKDY
                     RETLAATIGSLSHNEGRRWRPLEDTAAASHLLRGHSPNWNELRRGQDYYSEGALVWLE
                     ADAIIRELSEGKKSLDDFCRKFLGVNRTDVSVVPYELPEIVSKLRELADFDWESFFAK
                     RVSLPQDSLPLEVVGRCGYRLQYATEPPSGSPISRQRGGISARDSVGLSFSLEGQIVD
                     VVPGMTGDRAGLAPGMTVIGVNSKKFSRQRLLDALADSVALRRIELLLLEGEEFRTVV
                     LDYADGPRYLVIVRDESKPDVLANILKPLTGQPTGTGAQPAGARTDGASPRSRTGSGT
                     GSK"
BASE COUNT          436 a          714 c          738 g          408 t
ORIGIN      
        1 aggcgtcgtc gtcgaggcgg ccgtcgatcc ggatcggtgc cgaggcgcga tagcagacgt
       61 aacctttggg tgcggggccg ccgtctctac gtctgtcgtc gtcagcggcg ccgcgggacg
      121 tgccggcgat cacggccatg agtcccagca aaaccgcccg ccacttgtct ttgcgcatga
      181 attggtatcc tgagctcgtg atcgatttcg tgggcatggg cccgtaagac atcgtcgtca
      241 tttggaaccg gtgccggaac cggtgcgcga gcgcggactc gcgccgtcag ttcgagcccc
      301 tgcaggctgc gcccctgtgc ccgtgggctg gccggtcagt ggcttgagaa tattggccag
      361 gacatcgggt ttcgattcat cacgaacgat caccagatat cgcgggccat cggcataatc
      421 gagcacgacc gtgcggaact cttcaccctc gagcaagagc agctcgatcc tgcgaagggc
      481 gacactgtct gccagcgcat cgaggagtct ctgccggctg aacttcttgc tgttgacacc
      541 gatgacggtc atgcccggcg ccaggccggc acggtcgccg gtcatcccgg gtacgacgtc
      601 gacgatctgg ccttcgagac tgaatgagag ccccaccgaa tcgcgagcgg agatcccgcc
      661 ccgttgccgt gagatcggtg aaccgctggg cggctcggtc gcgtactgga gtcgataacc
      721 gcaccggccg acgacctcca acgggagtga gtcttgcggc agtgaaactc gcttggcgaa
      781 aaacgattcc cagtcaaagt cagccagctc gcgcagcttt gagacgatct ccggcagctc
      841 gtaagggacc accgagacat cggtgcgatt gaccccgagg aatttgcggc agaagtcgtc
      901 caggctcttc ttgccctcgg aaagctcacg gataatggcg tcggcctcga gccagacgag
      961 ggcaccctcc gagtaataat cctgaccgcg cctcagttcg ttccagttgg ggctgtggcc
     1021 tcgcagcagg tgagaagccg ccgccgtatc ctcgagcggc cgccaccgcc ggccttcgtt
     1081 gtgagagagt gaaccgatcg ttgctgccaa cgtctcccga taatccttcg aatccaccag
     1141 accggcgcga accatgagca cttctcccag gtactccgtc aggccctcat agacccagag
     1201 cagccgggtc ttgagcggcg tctgaaagtc aggcgtgcac atgccggccg gccggcggaa
     1261 cttgccgcac caggaatgga catattcatg agggatgagg ttggcgaccc agcccttccg
     1321 cttggagtcg tccagaaggt cccgctcgtg cacgccgttg atactgcacg cgagatgctc
     1381 cagcccgtgg tagccgaggt catcactgca cgtcaccagg aagtgaaaat cggggtaatg
     1441 gcaggcgccg aagagagcgc cggcctctct caccatccgg ctgtacatgt cgaccacgtt
     1501 gggtccgatc cgtaacgcgc tgggtgattc cgaaaccaga tcgaggaagg cgggcggata
     1561 gggaccggta ttgagcggga tcgcccgcag gtgttcgccg gcaatcaagg ggttgtcgac
     1621 gaggtcggtg accgtgacag tcttgaatgc gacgggcgcg tcaccggccg gcttggggac
     1681 ctgcgcatcg cgcggtgatt cggtcgccag tgcggtcgcg aaccgccagt cgcgcggcag
     1741 ccgcaggctc aagtgagccc gtatttgatc gcacgacggt ccgtcgggat agagcaggca
     1801 tgtgctccag ttgatcattc ccacggagtt attgccataa ctcaggtgac cagatgcctc
     1861 gaccgcggga ccattgcaga tgacatcgag ccgcgcgacg atctcgcgga caccatcggg
     1921 tacgtcgcac tcgacccggt agacgtcgat gtcattgcgg cgccacggta cgggtttacc
     1981 ttcgggcgtc tcgacgcgca gcccggctac atcctggatc gcgccgctgg gggcatgggt
     2041 acccgggatc cacttgggat accagagccc tagcttgcct ggctggcacg gcacgacaat
     2101 ccgcgcgtgc aaaagccggc gcggcaagtc gcgcgcgtcg acctcgatct gcagcgtcgg
     2161 atccgagccg gcctgcgcac tcggattagc aaggcagatc gacaccaggc tgagtagcca
     2221 agtcgagacc agcaggcaag gacgcgagtg caaacgctca gaggcacgcc gatcttgcag
     2281 tgtcattgcg gattca
//
LOCUS       DATAQL010001305         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4560619, whole genome shotgun sequence.
ACCESSION   DATAQL010001305 DATAQL010000000
VERSION     DATAQL010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4560619"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VHS97_22505"
     CDS             complement(<1..860)
                     /locus_tag="VHS97_22505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3451043.1"
                     /translation="MPALAALFIVFAVLGQDTKAAPRLSDGFQLQDFRGAIHSLDDVR
                     DRKMVVIAFLGVECPVVSQYAPRLVELAGKFEPKGVAFFAIDANQQDSPSAMGRFAKE
                     NALPFPFLKDVGNELADRLQVQRTPEVVVLDAERAIRYRGRIDDQFDRGVHRSAPLRS
                     DLAIVLEELLAGRDVSVAKTDAVGCRVGRIRRPGHGEVTYAKQVARILNDRCVACHRP
                     GDIAPFSLTTYKQAAGWADTIAEVVRDGRMTPWHVSPQYGKFRNDINLSDDEKRLIAA
                     WVADGAPAGDL"
     gene            1112..>2041
                     /locus_tag="VHS97_22510"
     CDS             1112..>2041
                     /locus_tag="VHS97_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3451044.1"
                     /translation="MRQIDPGRAARESTRRQFLQVGYSGLVGLGLPGLLAARAAAGAG
                     GVRPGRARSVIVILLSGGLGQHDSFDMKPDAPDGMRSEFKPIATAVPGIHICEHLPGL
                     AARLRKVAIVRSMSHPEGNHLVAVHRVLTGQPSNPRGASDLDRVASRNDFPCYAAVLD
                     LVRRRSDGVPSGVALPTRLVEGPLTWPGQDAGFLGPRHDPWQLRLDPAHPEARDDSLA
                     LPPGLDPVRLHSRRHLLAQGAAASPSDPFLDQQDAALAMLCNGRVSRALDLDREDPRL
                     IDRYGRHCFGQSLLTARRLVEVGVPIVQATMGIV"
BASE COUNT          350 a          641 c          660 g          390 t
ORIGIN      
        1 aggtcgcctg cgggtgcgcc gtcggctacc caggcggcga tgagtcgttt ctcatcgtcg
       61 ctcaggttaa tatcgttacg gaacttgcca tactgggggc tgacgtgcca aggggtcatg
      121 cggccatcgc ggacgacctc ggcgatcgta tcggcccatc cggcggcctg cttgtaagta
      181 gtcagagaaa agggcgcgat gtcgccgggt cgatggcagg caacgcagcg gtcgttcagg
      241 atgcgcgcaa cttgtttcgc gtaggtcacc tcgccgtggc ccggccgccg aatgcggccg
      301 acccggcagc cgaccgcgtc cgtcttcgcg accgagacat cgcggccggc aagcagctct
      361 tcaagcacaa tagccaggtc actccgcaac ggtgcggacc ggtgcacgcc ccggtcgaac
      421 tggtcgtcaa tgcgtccccg gtatcggatt gctcgctccg catcgagcac gacgacttcc
      481 ggagtccgct gcacttgcag ccggtccgcc agctcatttc cgacgtcctt caggaacgga
      541 aagggcaacg cattctcctt ggcgaaccgc cccatcgcac tgggactatc ctgctggttc
      601 gcgtcgattg cgaaaaacgc gactcccttc ggttcgaact ttccggccaa ctccaccagc
      661 cgcggcgcgt attgactgac gacgggacac tcgaccccga gaaaggcgat tacgaccatc
      721 ttgcgatcgc gaacatcgtc caacgaatgg atcgcgccgc ggaaatcctg gagctgaaag
      781 ccgtcggata gtcgcggcgc cgccttggtg tcttgcccga ggacggcgaa aacgataaac
      841 aaggctgcga gagcgggcat ggttgatcga ccttttctgc aacggcaata acttcgttta
      901 gtctatgagc tggggcaacc ggtccatcat catcctacca caaatgtcgt ggatggcgag
      961 aggcttctgg agatttggaa agcagattga cgatgatcgc gcgattggca ctggaacctt
     1021 ggcaaatact tgttaaactt ctaagcggca taccgagctg tggacgtgat cgcgacccac
     1081 cctgcggcga cgcaacgcga ggatcagcgt gatgagacag atcgatccgg gacgggcagc
     1141 ccgcgagtca acccgtcgtc agtttcttca ggtggggtac tcggggctcg tggggctagg
     1201 attgccggga ctgctggcgg cgcgtgcggc cgcgggggct ggcggcgttc ggccgggacg
     1261 agcgcggtcc gtcatcgtga ttctcttgag cggcgggtta gggcagcacg actcgttcga
     1321 catgaagccc gatgcgcccg acgggatgcg cagtgagttc aagccgatcg cgacggcggt
     1381 gccgggaatc catatctgcg aacatctgcc cggtctggcc gcgcggctgc gtaaggtggc
     1441 gatcgtccga tcgatgtcgc acccggaggg caaccacctg gtggcggttc accgcgtctt
     1501 gaccggccaa ccctcgaatc cgcgcggggc cagcgacctc gatcgggttg cttcacggaa
     1561 cgatttcccg tgttatgcag ccgtgctcga cttggtgcgg cggcggagtg atggcgttcc
     1621 cagcggcgta gccttgccga cccgtttggt cgagggaccg cttacttggc cgggacagga
     1681 cgctggtttt ttgggtcccc gccacgatcc ctggcaactg cgcttggacc ccgcccaccc
     1741 cgaggcccgc gatgacagcc tggccctgcc gcccggtctc gatccggtcc ggctccacag
     1801 tcgccgccac ctgttggcgc agggcgccgc ggcctcgccc agtgatccct tcctcgatca
     1861 gcaggacgcc gcgctcgcca tgctctgtaa tgggcgtgtc agtcgcgctc tcgatcttga
     1921 ccgtgaagac ccgcggctga tcgatcgata tggccgtcat tgttttggtc agtcgctctt
     1981 gactgcgcgg aggctggtcg aggtcggcgt gccgatcgtc caggccacga tgggcatcgt
     2041 c
//
LOCUS       DATAQL010001306         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4866906, whole genome shotgun sequence.
ACCESSION   DATAQL010001306 DATAQL010000000
VERSION     DATAQL010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4866906"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1313
                     /locus_tag="VHS97_22515"
     CDS             195..1313
                     /locus_tag="VHS97_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451045.1"
                     /translation="MLQRRRGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRIQCT
                     NNLKQLGLALHNYESSNNSFPVASVFGVGTFAMPGVQCTSPGFGNNCQNTPWFVLMLP
                     YIEQGPLYNSFNAGFGMEGPALGGFLINSTVYTTKIPSFQCPSDNLQTFSFAALSKAT
                     GGSVPAFPWSPTKGNYGVNWGNTDYGQAAFGAFKTVYRQAPFGVNTTGSAPQLVRIAS
                     VTDGLSNTHFVAELLQGAIDDVRGTIWVDNPGAGSYFTRFTPNGNQDVLQLLGVWTGN
                     ATNNADNVASFAGTSVGTSPPGPGSLCDSQPAQLLGCNNQGSEGQEYTASRSRHPGGI
                     NALFGDGSVRFVKNSIGAQTWVAIGTINSGEVVSSDSY"
     gene            complement(1395..2525)
                     /locus_tag="VHS97_22520"
     CDS             complement(1395..2525)
                     /locus_tag="VHS97_22520"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX3451046.1"
                     /translation="MARRERPPKDSPVEDPAPIEVLVVDDSALVRQRLKSIIESDTQF
                     RVVLAADPYEAVAMLSKSVPGVIVLDVEMPRMDGLTFLRKLMRQHPMPVVLCTSQAER
                     AVTALQMGAIEVIAKPDWNNANRLTDWSQNLLESIRNAARAGRLPFRDDRPASATDSR
                     HTADVVLPKIPYSPRAGIHEKIVVCGVSTGGVQALQQLLAGFPATAPGIVIVQHMPAA
                     FTTEFAKRLHNDPRIELEVAEARHHEPIGAGRALVVPGDFHGLVRRSGTGYRVELVEG
                     PPVCRHRPSVEVLFRSAAQAAGPNAAGVIMTGMGDDGAIGLLEMREAGALTIAQNEAT
                     CVVFGMPREAIRRGAAKFVIPLESIAPTVMAWVGSADSGTWH"
     gene            complement(2532..3395)
                     /locus_tag="VHS97_22525"
     CDS             complement(2532..3395)
                     /locus_tag="VHS97_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX3451047.1"
                     /translation="MATSGVLPLPPADPEAWSEKDFVPITAFLRHQIGITLEAHRMGL
                     LQARLRSRLQVKGFNSFNQFYDQVLKPDPTGWGAQLLIDLSTVNHTSFFREPGHFTFM
                     AERVAAMLKESPATTVRIWSAGCSSGQEPYSMAMVLAESLINLPPQKVEIWASDLSLD
                     MLKSAAQAIYTTRDVQGVSPARLRRFFLLGKGPREGSFRVVPEVRDVVKFRHIDLRTP
                     SWPIPSDFPIIFCRNVSIYFADDERLTLLDRLAQHLRPGGWLAMGNGEILPDVPPTLR
                     KHSPSLFQKEG"
     gene            complement(3397..3903)
                     /locus_tag="VHS97_22530"
     CDS             complement(3397..3903)
                     /locus_tag="VHS97_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3451048.1"
                     /translation="MVDVETEASTDADRERASSYVVFQLGGEGYALEVMRVQEVKDML
                     ALTEVPGGPKSLLGVINLRGHVVPVYDLRLPFGLSKETKATRAPCVLIVESNVGSDVQ
                     ITGLLVDRVSDVLEFSPEEVQAAPQLGLGKATPFVRGLIRHQDAFLLVLDLDRIFSTL
                     GSLNGEGV"
     gene            complement(4024..>4587)
                     /locus_tag="VHS97_22535"
     CDS             complement(4024..>4587)
                     /locus_tag="VHS97_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451049.1"
                     /translation="GLLDRTVPMIRDTSILIQEIAAASQEQMAAIREINMGVSQLEEV
                     VQQNAAASHDLFNTATGMADQSTRLKRLVDFFQLDTSGSGWTGPGGNGAQPRSRPGAA
                     MRAHPSPVKPLPAPGRRAIPSHGFDGQQPSGHGGGGLDHPAPAPVLPPQHGPAAPSVH
                     PSSNSGTSPRGGVVVNLDDDDNFERFS"
BASE COUNT          917 a         1406 c         1371 g          893 t
ORIGIN      
        1 cacgacgtct caaaccgcag atttatgaac taggccctcg caaatcaaaa gcaggaatgt
       61 tagtttgaat gtgacgacgt ttaagtattt cggagaatat ggatcgtcgt cacctttctc
      121 atcaccgggc tcgtgcgtgc ccggttcgtt ttcgacaaga tatacaactc tgatttcggg
      181 ggggtggtga agccatgtta caacggcgtc gtggttttac gctgatcgag ctcttggtgg
      241 tgatcgcgat catcgctgtg ttgatcgcct tgctcttgcc agccgtgcag gcggcgcgcg
      301 aggccgccag acggatccaa tgcaccaaca atctcaaaca gttggggctg gctctgcaca
      361 actacgagtc atccaacaac tcgttccccg tcgcctcggt cttcggcgtg ggtaccttcg
      421 caatgccggg ggtccaatgc acgagtccag gattcggcaa caactgtcag aacacgccgt
      481 ggttcgtcct gatgctgccg tatatcgagc agggccctct ttacaactct ttcaatgccg
      541 ggtttgggat ggaggggcct gcgctcgggg gcttcctcat caacagcacc gtttatacga
      601 caaagatccc ctcgtttcag tgcccgagcg ataatcttca gacgttcagc ttcgcggcat
      661 tgtcgaaagc gactggcggc tcagtccccg cgttcccctg gtcgccgacc aagggaaatt
      721 atggcgtcaa ttggggcaat acggattacg gccaggctgc cttcggagca ttcaagaccg
      781 tgtaccgcca ggctcccttc ggcgtcaaca caactgggtc ggccccccag ttggtccgga
      841 ttgcgtctgt caccgacggt ctaagcaata cgcatttcgt ggccgagctt ctccaggggg
      901 ccattgacga cgtccgaggg acgatctggg ttgacaatcc cggcgcgggc tcgtacttca
      961 cgcgcttcac ccccaatggc aaccaagacg ttttacagct tctgggagtg tggaccggca
     1021 acgccaccaa caacgccgat aacgtggcgt cgttcgccgg aacgagcgtg ggaaccagcc
     1081 ctccgggtcc cggctcactc tgcgacagcc agccggctca gctgttgggc tgcaacaacc
     1141 aaggatccga ggggcaggag tacacggctt cgcgaagccg gcatcccggc ggaatcaacg
     1201 ctctcttcgg cgacggctcg gtgcgtttcg taaagaactc gatcggcgcc cagacatggg
     1261 tcgcgatcgg aacgattaac agtggcgagg tggtcagctc tgattcttac tgaggaaccc
     1321 tcgagcgaat agagagatta ggaaacaagg cgattggcga tcgagcgcca atcgccttgt
     1381 tctttcattc ccttctaatg ccacgtcccc gaatcggcac tgccgaccca ggccatgaca
     1441 gttggcgcga tcgattcgag cggtatcaca aacttggccg cgccgcggcg gatggcctcg
     1501 cgcggcatgc cgaaaacgac gcacgtcgct tcgttctggg cgattgtcag tgcgcctgcc
     1561 tcgcgcattt ccaagagacc gatggcgccg tcgtcgccca tgcccgtcat gatcacgccg
     1621 gccgcgttcg ggccggccgc ctgcgccgcc gaacgaaaca agacttccac gctggggcgg
     1681 tggcgacaga ccggcggacc ttcgaccagc tcgacccgat agcctgtccc cgaccggcga
     1741 acgaggccgt gaaagtcacc cgggaccacc agcgcccggc ccgccccgat cggctcatgg
     1801 tgccgggcct cggccacttc aagttcgatc cggggatcgt tgtgcagacg cttggcaaac
     1861 tcggtcgtga aagcggcggg catgtgctgc acgattacga tccccggcgc tgtcgcggga
     1921 aagcccgcca gtaattgctg caaggcttga acacctccgg tgctcacgcc gcaaacgacg
     1981 atcttttcat ggatgcccgc gcggggtgaa taaggaatct tgggcaaaac cacatccgct
     2041 gtgtgcctgg aatcagtcgc actcgcgggt cgatcgtctc gaaacggaag ccggcccgcg
     2101 cgcgcggcgt tgcgaatgct ctccagcagg ttttgcgacc agtccgtcaa acggttcgcg
     2161 ttgttccagt cgggcttggc gatgacctcg atcgctccca tctgtagcgc ggtcacggcc
     2221 cgctccgctt gcgaagtaca gagcacgacc ggcatcggat gctggcgcat cagcttgcgc
     2281 aagaacgtga ggccgtccat ccgtggcatc tcgacgtcga ggacgatcac tcccggcaca
     2341 gacttgctga gcatcgcgac cgcttcgtag ggatcggcgg cgagcacaac ccgaaactgc
     2401 gtgtcggatt caatgatcga tttcagccgc tgccgcacca gtgcgctgtc gtcgacgacc
     2461 agaacctcga tcggtgccgg atcctcgacg ggcgaatctt tcgggggccg ttcgcgccga
     2521 gccatgcccg cttaaccctc cttctgaaat agcgacggag aatgtttgcg gagtgtgggg
     2581 gggacatccg gtaaaatctc gccgttgccc atggccaacc agcctcccgg tcgcaagtgc
     2641 tgcgccaagc ggtccaacag tgtcagccgt tcgtcgtcgg cgaaatagat cgatacgtta
     2701 cggcaaaaga tgatcggaaa atccgagggg atcggccacg acggggtgcg cagatcgatg
     2761 tggcgaaatt tcacgacgtc gcggacctcg ggcacgacac ggaatgagcc ttcgcggggg
     2821 ccttttccta acaagaagaa ccgacggagt cgcgccggcg aaaccccctg aacatcacga
     2881 gtcgtgtaaa tcgcctgggc ggccgacttg agcatatcaa gcgaaaggtc actggcccaa
     2941 atctctactt tttgcggcgg gagattgata agtgactcgg ccaggaccat cgccatgcta
     3001 tagggttctt ggccggagga gcagcccgcc gaccagatcc ggacggtcgt cgctggcgac
     3061 tccttgagca tcgcggcgac acgctcggcc atgaaagtaa agtgcccggg ctcgcgaaag
     3121 aacgaggtgt ggttgaccgt ggacaggtcg attaagagtt gcgcgcccca gcccgtcggg
     3181 tcgggcttca agacctggtc ataaaactga ttgaacgaat tgaaaccctt gacctgaagg
     3241 cgcgaccgga ggcgggcctg caagagaccc atcctgtggg cctcgagggt gatgccaatc
     3301 tgatggcgca gaaacgccgt gatcggcaca aagtctttct cagaccaggc ttcgggatcc
     3361 gccggcggga gtggcagcac gcccgatgtc gccacgttac actccctcac cattcagcga
     3421 ccccaaggtc gagaaaatac gatctaagtc caatacgagc aagaaggcgt cttggtgacg
     3481 gatcaagccg cgcacgaagg gggtcgcttt cccaagtcca agctggggtg cggcttgcac
     3541 ttcctcaggg gaaaactcga gtacgtcgga aacccggtca accagaaggc ccgtgatctg
     3601 tacgtcgctt cccacgttgg actcgacgat gaggacgcag ggcgcacgcg ttgccttggt
     3661 ctctttcgac aggccgaatg gtaagcgcaa atcgtagacc ggaacaacat ggccgcgcag
     3721 gttgattacg cccaagagag atttcggtcc gccgggcact tcggtcagcg cgagcatgtc
     3781 cttcacttcc tgaacgcgca tcacttcgag agcgtaaccc tctccaccca attgaaacac
     3841 gacataggac gacgctcgct cgcggtcggc gtctgtactg gcctccgtct cgacatcgac
     3901 catcgtccca ccctcttgat cgtgtgcact agataatcta ggcgacttgg cgcccgaatc
     3961 ggctctcaac tgcggactga atcgcgtttt accgacatgt tttattaagg accgatcacc
     4021 ctatcacgag aatcgctcga aattatcgtc atcgtcaagg ttaaccacga ctccgccgcg
     4081 cggggacgta ccggaattgc tggatggatg gactgacggc gcggccgggc catgctgtgg
     4141 cggcaacacc ggcgccggag cgggatgatc cagaccgccg ccgccatggc cgctcggttg
     4201 ttgtccgtcg aatccgtggg aaggaattgc tcggcggccc ggtgctggga gcggcttgac
     4261 cgggcttggg tgcgctcgca tcgccgcccc gggacgcgag cgcggctgag ccccatttcc
     4321 cccgggcccc gtccagccac ttccggatgt atccagttga aagaagtcga ccaggcgctt
     4381 cagtcttgtc gactggtcgg ccatcccggt cgccgtgttg aacaagtcgt ggctggccgc
     4441 cgcattctgc tgcacgactt cctcgagctg gcttaccccc atgttgatct cgcgaatcgc
     4501 cgccatttgt tcctgtgagg cggcagcgat ctcttggatg agaatcgatg tatcgcgaat
     4561 catcggcacc gtccggtcca gaagccc
//
LOCUS       DATAQL010001307         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1052000, whole genome shotgun sequence.
ACCESSION   DATAQL010001307 DATAQL010000000
VERSION     DATAQL010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052000"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VHS97_22540"
     CDS             <1..165
                     /locus_tag="VHS97_22540"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEX3451050.1"
                     /translation="PKGNDLNELATRLKNTCGAGGTVKDGQIELQGDHLQAAERTLQA
                     IGYKTKARGG"
     gene            334..1188
                     /locus_tag="VHS97_22545"
     CDS             334..1188
                     /locus_tag="VHS97_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451051.1"
                     /translation="MRSLPLVICLLALEASTTSATNPTEEGLTLSWADNILNVRSRDL
                     PGGAVKVWYLEAFCRPGSTARDWAETVIPHGTKLIQEEPDSRRIRLRSTLEDGVIIEH
                     DIRAGRDEVDFRLTATNPTRRASQAHWAQPCVRVDAFTGVKPMRNSEEYLPKCFIFVD
                     GRLTRLPTQPWATKAKYTPGQVWCPAHVDRDDVNPRPLSELVPSSGLVGCFSADGKHV
                     LATAWEPYQELFQGVITCLHSDFRIGGLAPGETKSIRGKLYLTGPDVEALVKRYEADF
                     PEHHRRGR"
     gene            1506..3227
                     /locus_tag="VHS97_22550"
     CDS             1506..3227
                     /locus_tag="VHS97_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3451052.1"
                     /translation="MQRPSIVSQGSWIVMLFGIATFGSGCAEQAARNSARSAAGATST
                     ADRVNSRLTPDSENFLKYSSPPSGNTSQSSFLHLAKERLTTDEQAKSVDAAELPAELN
                     SEAYDRIVDNPFRRTANEPLSTFSIDVDTASYANVRRFLSQNMLPPKDAVRIEEMLNY
                     FAYRDAPPATASEHPFAVHVEVADCPWNSQNRLARIGIAARPIDQAQRPPSNLVFLID
                     VSGSMQEPNKLPLVQWSLQRLVEQLGENDMVAIVVYAGASGLVLPSTSCLHKAEIMSA
                     IDQLRAGGSTNGGAGIQLAYDVATRHFIKNGTNRVILATDGDFNVGVSNHGDLIRLIE
                     AKAKSGVFLSVLGYGMGNIKDGNLEKIADKGNGHYAYIDSPREAYKVLVEEMGSTLVT
                     VAKDVKIQVEFNPAKVGAFRLIGYENRIMAHQDFNDDTKDAGEIGAGHHVTALYELVA
                     PGKEGDLPGIDALKYAKPASSSSPSNESFTVKIRYKRADGDTSRRLEYGVVDEGRSFA
                     QASDDLKFASAVAGFGMLLRDSADKGSLTYPGILEIAESTLSKDSSGYRQEFVAAVRQ
                     AQALTRR"
BASE COUNT          710 a         1054 c         1018 g          595 t
ORIGIN      
        1 ccgaagggca acgacttaaa tgagctcgcc acgcgactca agaatacttg cggtgcgggc
       61 ggtaccgtaa aggatgggca gatcgagctt cagggcgatc acctccaggc ggctgagcgc
      121 acgctccagg cgatcggcta caagaccaag gcccgcggcg ggtgattccg ctcggggtga
      181 ctcaaaacga aaaagacttg tgattcgctt cgtcaccggc cagcatggcg atgcagaggg
      241 tcgaaccatc tcaaccttta tcggcgagct gatagccggc ttctatcgcg attagccccc
      301 aacgtagggg cctgagccat cgaggagatg cccatgcggt ctctgcccct ggtgatctgc
      361 ctgctggccc tcgaggcgag cacgaccagc gccacgaacc cgaccgaaga gggactgacc
      421 ctctcctggg ccgacaatat attgaacgtc cgcagccggg atctacccgg cggcgcggtc
      481 aaggtctggt acctggaggc gttctgtcgg cctggttcga ccgctcgcga ctgggccgag
      541 actgtgatcc cgcacgggac caagctgatc caggaagagc cggacagccg tcgaatccgc
      601 ctccgcagca cgctagagga tggggtaatc atcgagcacg acatccgagc cggccgcgac
      661 gaggtcgact tccgcctgac cgccaccaac ccgacgcgcc gggcctcgca ggcccactgg
      721 gcccagccct gcgtgcgcgt cgacgcgttc accggggtca agccgatgcg gaactccgag
      781 gagtatctgc ccaaatgctt catcttcgtc gacgggcggc tgacgcggct gccgacgcag
      841 ccgtgggcga cgaaggcgaa gtatacgccc ggccaagttt ggtgcccggc ccacgtcgac
      901 cgggacgacg tcaaccctcg ccctctcagc gaactcgtgc cgtccagcgg cctggtcggc
      961 tgcttctccg ccgatggcaa gcatgtcctg gcgacggcgt gggaaccgta tcaggagttg
     1021 ttccagggcg tcattacctg cttgcactcc gacttccgaa tcggtggcct tgcgccgggc
     1081 gagaccaagt ccatccgggg caagctctac ctgaccgggc cggacgtcga ggcactcgtg
     1141 aagcgctacg aggccgactt tcccgagcac caccgccggg gccgttaacc gggacgatgg
     1201 ctattgaatg gactcgcaac ggaaggacat gaggagtgtt ccagcggacg cgcatcgcaa
     1261 ggttgcaaag tcaatgtacg taatgtctct cccatagaac gggaaccaac ccgtcgcgtg
     1321 agcgtctaag gaaagacggc tggaccgttg aacgggtgaa agggcaatca tcatcgaccg
     1381 tcgttgcccg gtgcaacgct gttcgctccg ttagacctgg gtctgtcaga ccgagcacgt
     1441 cttcttctcc aatcttcgcc ctttgccgcg atccatgtcg gccgtcccat tcgaggacac
     1501 accccatgca gcgcccatcg atcgtttcac agggtagttg gatcgtgatg ctgtttggaa
     1561 tcgcaacctt cggctcaggt tgcgccgaac aagcggcgag gaattccgcc cggagcgcgg
     1621 ctggcgcgac gagcaccgca gacagggtca actcgcgact cacgcccgac agcgaaaact
     1681 ttctcaagta ctcaagtcca cccagcggga acacgagtca atcgtccttc ctgcatctcg
     1741 caaaagagcg cttgacgaca gacgaacagg caaaatcggt ggatgccgcc gagttacctg
     1801 ccgagctgaa cagtgaggct tacgaccgca tcgtcgacaa tccgttccgt cgcaccgcca
     1861 acgagccact ctcaaccttc tcgatcgacg tcgacaccgc cagctacgcc aacgtgcgcc
     1921 gctttctcag ccagaacatg cttccgccca aggatgcggt gcgcatcgaa gaaatgttaa
     1981 actactttgc gtatcgcgac gcgcctcctg cgacggcgag cgagcatcct tttgcggtcc
     2041 atgtcgaggt cgccgactgc ccctggaaca gccagaaccg gctggcccgc atcggcatcg
     2101 ccgcccgacc catcgatcaa gcccagcgtc ctccgagcaa cctcgtcttt ttgatcgacg
     2161 tctcgggctc gatgcaagag cccaacaagc tgccgctagt ccagtggagc cttcagcgcc
     2221 tggtcgagca gctcggcgag aatgacatgg tcgcgatcgt ggtctatgcg ggtgcgtcgg
     2281 gtttggtgct accatcgacc tcatgcctgc acaaagcgga aatcatgtcg gccatcgacc
     2341 agttgcgcgc cggcggctcg acgaacggcg gagcgggcat tcagctcgct tacgacgtgg
     2401 ccactcgaca tttcatcaag aacgggacca accgcgtgat tctcgccacc gacggtgatt
     2461 tcaacgtcgg cgtctccaat cacggagacc tgatccgact gatcgaggcc aaggcaaaga
     2521 gcggcgtctt cctgagcgtc ctggggtatg gcatggggaa tatcaaggac ggaaatctcg
     2581 agaagatcgc tgacaagggc aacggccatt atgcctacat cgactcgccg cgcgaggcct
     2641 acaaagtatt ggtcgaggag atgggctcga cgctggtcac ggtcgccaag gacgtgaaga
     2701 ttcaggttga attcaatccg gcgaaggtgg gcgcattccg gctgatcggc tacgagaatc
     2761 ggatcatggc tcaccaggat ttcaacgacg acaccaagga cgccggcgag atcggcgcag
     2821 gccaccatgt gaccgcgctt tacgagctgg tcgcgcccgg caaggagggt gacctgccgg
     2881 ggatcgacgc tcttaagtac gcaaagccag cttcgtcaag ttcgcctagc aacgagtcct
     2941 tcaccgtgaa gatccgttat aaacgagccg atggcgacac cagccgccgg ctggaatacg
     3001 gtgtcgttga tgaaggacga tcgttcgccc aggcatcgga cgacctcaag ttcgcctccg
     3061 cggtcgccgg tttcgggatg ctgctgcgcg attccgccga taaagggagc ttgacctatc
     3121 cgggaattct tgagattgcc gagtcgactt tatccaaaga ctcatccggc tatcgtcagg
     3181 agttcgtcgc ggctgttcga caagcgcagg ccttaaccag acggtgagaa tggagaatag
     3241 cagaacatag attggaagcc caaacgacac tcaatctcct cgatcgcggg caaaagcgac
     3301 gaagccattg gccgggatga ctacggtcgc cgagcagggc aggccgtgca tcggagggcc
     3361 atcggcgttg acgcccg
//
LOCUS       DATAQL010001308         5563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2427809, whole genome shotgun sequence.
ACCESSION   DATAQL010001308 DATAQL010000000
VERSION     DATAQL010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427809"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1223)
                     /gene="argC"
                     /locus_tag="VHS97_22555"
     CDS             complement(195..1223)
                     /gene="argC"
                     /locus_tag="VHS97_22555"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX3451053.1"
                     /translation="MTTKIGVAIVGASGYAARELIGILLGHPRVRVTVATTRQDELPR
                     LDALHPSLARRIDLVCEPFDADRIAERATFAFLALPHTASMAVVPSLRQRGVRVIDLS
                     ADYRLRDPQVYADWYGHAHTDPEGLRAAVYGLPELFRDRIVPVDLVANPGCYTSASIL
                     ALAPLVAEDLIERSSIIIDAKSGVSGAGRSPKLMYHFPECNESLSAYSVGQHRHTPEI
                     DQVLTAVAGGRGDPVEVVFTPHLVPMDRGIFATIYAQPKAHVTEHDLLALYRSFYARS
                     PFMRVGGHLPATKDSAFTNFCDITVRVVRGRVLVLASLDNFIKGAAGVAVQNFNLMTG
                     CPEETALF"
     gene            complement(1225..3849)
                     /locus_tag="VHS97_22560"
     CDS             complement(1225..3849)
                     /locus_tag="VHS97_22560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451054.1"
                     /translation="MRSKSFVLTSPGFRTGLAGGLAVVLILVAWLWPIGIGGKMPVGG
                     DVTQFFIGLMGFLSESLRQGRLPVWNDLWGFGFPGVAESQMGVFYPVHVILYRWLETE
                     TAYVVSLVAHTLWGGLGTYWAARRLKVSCTGAALAAGSWSTCGFFVIHLAHPWGYTTG
                     CWMPWAWGLAWCSLAPASAFQKIAPLLLSLVLVLQVLPGHFQLAFLTQFGILLFVLWM
                     VTEHWVGRARAAPTTPRDTAVTNLRGLGRVFLAMAAVFPLAAVQLWPTARLAGLAASQ
                     RDFEYMSGFASTPFHLVNYVAPGLFHRSPLWRPLVWTPFHTAPEEHLAYIGLVPLFLA
                     VMAMVREWRNDRTVRLLAILFFVTLCLSLGPYLPGFRQTIMVPGFSFFRAPSRWSLAS
                     ALALAILAGKGFDRWLEWPSAGRSLQRLNFLAFFWVLATVGIIELALASTRPPGWPAV
                     ARGFQRVFDAMPWNGDPTIANRDTTFAAVMAGAREQPDLPVVPVGLSRAMFLQENKSD
                     GSFASERVMIYSRELVELAALLIALWFIARLSERDMTGRARSRWALVALAFVDLWVLG
                     RHRLVDLGPLKPLAEQSPVLARLTREPRGTRIADDKLKNLPMLVGLAPLAAYRTLDLP
                     AIPELTSLTRAPTTAPAIAPYVTAAFRATGTGLRVFDPIENRREQLLGRGSIAKERID
                     DPALATWLFGATWVADHPWARAFSIWRAENPPVRAWLVPLDSIDSPVRLEESSGDPRE
                     ILKIIDSAAPLESESARPDELAISVKTDDPAWVIVSQLADPQWTALWVGLDEQGKLAG
                     EILPAFRRGTHPGGWQRIAVPGSGRWTLRLKYDARDVADGAVVSTIAWLCWLMAAFWM
                     TIQYGRGGRSPGRNQTEA"
     gene            4198..4386
                     /locus_tag="VHS97_22565"
     CDS             4198..4386
                     /locus_tag="VHS97_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEX3451055.1"
                     /translation="MLVLSRKLGQSFHIGPEIRVTIIKIDRNSVRIGIEAPDEVSVQR
                     EEIAFEVPEPRFLKVGAA"
     gene            4549..>5563
                     /locus_tag="VHS97_22570"
     CDS             4549..>5563
                     /locus_tag="VHS97_22570"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX3451056.1"
                     /translation="MRVVIAPDKFKGSLTALEAAAAMAVGVARAAPEASIDQVPMADG
                     GEGTTQALVAATGGSFREAQVTGPLAEPIIACFGLLGDGRTAVIEMAAASGLVLVPPE
                     KRNPLVATTRGTGELLLAAIAAGAGRVVIGIGGSATNDGGAGFGQAIGFRLLDDQDRD
                     LQPGGGNLARLARIDRLARRSELEGVEIAVACDVTNTLCGPRGASAVYGPQKGANPSM
                     VEILDQNLAHFAAVVERDLGVAIKDLPGSGAAGGLGGGLVAFAGGKLEPGVELIVDAV
                     KLADRLESADLCLTGEGAIDAQSAFGKTAVGVAQLARSLHCPTLALAGSIGPGARAVL
                     EHGI"
BASE COUNT         1165 a         1771 c         1692 g          935 t
ORIGIN      
        1 gttcacgccg gtcgccgtga acgtgccgtt gccgtgccat gttggcgatt tttgttgaac
       61 gggttgatcg gatagagcgc ccaagacagg gtgtcgaacg cgatggcatg acccagcaaa
      121 caaagcaatt ggaatccgtt ggggtgaatt tgccgttggc aagttacgct gcgcgacgac
      181 ttcggcgggt gcacttagaa caacgccgtt tcctctgggc acccagtcat caggttgaaa
      241 ttttgaaccg ccacacccgc cgcccccttg ataaaattgt ccagggacgc cagaacgagg
      301 acccgcccgc ggacgactcg cacggtaatg tcgcaaaagt tcgtgaacgc cgaatccttg
      361 gtcgcgggca agtgcccgcc gacccgcatg aacggactcc gcgcgtagaa cgagcgataa
      421 agcgccagca agtcgtgctc ggtcacatgt gctttgggct gagcataaat cgtggcaaag
      481 atgcctcggt ccattggcac caggtggggg gtaaaaacca cctcgacagg atcgccacgg
      541 ccccccgcaa cggccgtcag cacctggtcg atctcggggg tgtgacgatg ctgccccacg
      601 ctgtaggcgg acaggctctc gttgcactcg gggaaatggt acatcagctt gggcgaacgt
      661 cccgcgcccg aaactccgct cttggcgtcg atgatgatgc tcgaccgctc gatcaggtct
      721 tcagcgacca gaggagccag agccaggata ctcgccgagg tatagcagcc ggggttggcg
      781 accagatcga ccggcacgat ccggtcacga aagagctccg gcaatccata tacggcagcg
      841 cgtagcccct cagggtcggt gtgcgcgtgg ccataccagt cggcgtagac ctgggggtcg
      901 cgcaaccggt agtcggcgct caagtcgatg acccgcactc cgcgctggcg cagggagggt
      961 accacggcca tgctcgccgt gtgcggcaga gcgagaaagg caaaggtagc gcgttcggca
     1021 atccgatcgg cgtcgaacgg ctcgcagaca agatcaatcc gccgtgccag gctcggatgc
     1081 aacgcgtcca agcgcggcaa ctcatcctga cgcgtcgtcg cgaccgtaac tcggacccgt
     1141 ggatggccca gcaatatgcc gatcaactcg cgggccgcgt atcccgacgc ccccacgatc
     1201 gccacgccga ttttggttgt cacttcaggc ctcggtctgg tttcggcccg gcgaccttcc
     1261 gccgcgcccg tattggatcg tcatccaaaa agccgccatg agccagcaga gccatgcaat
     1321 ggttgaaacg accgctccat ccgccacatc gcgcgcatcg tactttaatc gtagcgtcca
     1381 gcgccccgag ccgggaaccg cgatacgctg ccagccaccg ggatgcgttc ctctgcggaa
     1441 tgctgggagg atttcgcctg ctaacttgcc ttgttcgtcc aggccgaccc agagcgccgt
     1501 ccattgcggg tcggcaagct gcgaaacgat cacccatgcc gggtcgtccg tcttgaccga
     1561 aatggccaat tcatccggcc gagccgactc agactcgagc ggagcggctg aatcgatgat
     1621 cttgaggatc tcgcgaggat cgcccgacga ttcctcgagc ctcaccgggc tgtcgatcga
     1681 gtccagaggg accagccagg cgcggacagg tggattctcg gcgcgccaga tcgaaaatgc
     1741 gcgcgcccag gggtgatcgg ccacccatgt tgcgccgaaa agccaggtcg ccagcgccgg
     1801 gtcgtcgatc ctttccttgg cgatgcttcc tcggccgaga agttgctccc tccggttttc
     1861 gatggggtca aaaacgcgca gccccgttcc agtcgcccga aacgccgctg tgacataggg
     1921 cgctatcgcc ggcgcggtcg tgggcgctcg cgtcagcgag gtcaactccg gtatggccgg
     1981 caagtcgaga gtgcggtacg cggcgagagg tgccaacccg acgagcattg gcaagttttt
     2041 cagcttgtca tccgcgattc gcgtcccgcg cggctcacgt gtcagcctgg ccagaactgg
     2101 gctctgctcg gccagaggct ttagcggtcc caggtcgact aaacgatggc ggcctagaac
     2161 ccagaggtca acaaatgcga gcgcgacaag cgcccaccga gaccgcgccc tgccggtcat
     2221 atctcgctca ctcaatcgag caatgaacca gagggcgatc agaagggccg ccaactctac
     2281 cagctctctg ctgtagatca tcactcgttc actggcaaaa ctaccgtcac ttttgttctc
     2341 ctgaagaaac atggcccgac tgaggccgac gggaacgacc ggtaggtctg gctgttcacg
     2401 cgcaccggcc atcaccgccg cgaacgtggt gtctcggttg gcgatcgtgg gatctccgtt
     2461 ccagggcatc gcatcaaaca cgcgctgaaa ccctcgcgcc actgccggcc agcccggtgg
     2521 cctcgtgctc gcgagcgcca gctcgattat ccccacagtc gccagcaccc agaaaaaggc
     2581 caagaaattc aagcgctgca gcgatcgccc tgcactcggc cattccagcc atcgatcgaa
     2641 acccttgcca gccaggattg ccagcgccag agccgaggcc aggctccacc tcgagggagc
     2701 gcgaaaaaac gaaaagcctg gcaccatgat cgtctgacga aaccccggga gataaggccc
     2761 aagactcaag caaagcgtca cgaaaaagag gatcgcgagc agccggactg tccggtcatt
     2821 gcgccattcg cgaaccatgg ccatcacggc cagaaacaag ggaaccaaac cgatgtacgc
     2881 cagatgctcc tccggggcgg tgtgaaacgg ggtccacacc aacggccgcc atagcggcga
     2941 gcgatggaac aagcccggcg cgacgtagtt aaccaggtga aacggagtcg atgcaaagcc
     3001 cgacatgtac tcgaaatcac gctgtgaggc cgcaagcccc gccagccgcg ctgtcggcca
     3061 aagctggaca gcagccaggg ggaacaccgc ggccatggcc aggaagacac gccccaaccc
     3121 tctcaggttc gtaacggcgg tgtctcgcgg cgtagtcggc gcggctcgcg cacggcctac
     3181 ccagtgttcc gtcaccatcc acaagacgaa gaggagaatc ccaaactgcg tcaggaacgc
     3241 cagttgaaaa tgcccaggca atacttgaag aaccagcacg aggctcagca ataacggcgc
     3301 gattttttga aaagcgcttg ccggggcgag gctgcaccac gccaagcccc acgcccaggg
     3361 catccagcaa ccggtcgtgt acccccaggg atgggccagg tgaataacga agaacccgca
     3421 cgttgaccac gaacccgcgg ccagggccgc accggtgcaa gagactttca accgccgggc
     3481 agcccagtat gtcccgagtc caccccagag ggtgtgagct acgagactca caacatacgc
     3541 agtctcggtt tctagccatc ggtagagaat cacatgaact gggtaaaaca cgcccatctg
     3601 actctcggcc acgccgggaa agccaaagcc ccagagatcg ttccagaccg gcagccgacc
     3661 ctggcgcagc gactcgctca gaaaacccat caggccgatg aaaaactggg tcacatcgcc
     3721 gcccacgggc atcttgccgc cgatgccgat cggccagagc cacgccacga gtatcagcac
     3781 gacagcaagc ccgccagcca atccagtcct gaaaccaggc gaggtcagaa cgaacgattt
     3841 cgacctcacc ctattctcca ttgcgagacc cctctctaaa aaaggttgct gcctgcatgc
     3901 aagttcggcg agacagtccg ccgcttacga tggcggtcaa ctcaaggaaa ttctttcaaa
     3961 atcagaatcg attttacacg ggaaaccgca aaacccgtat attcacgtcc accggaattg
     4021 tcgtctcaat ccaatcgtca actggaagtt acgctgttcg gctcgttcgg acgtatttca
     4081 cgtcgctttt ggcacgctag ctgctttggt taccgttcag aacaaacgca gatttacctc
     4141 tcgctttgtc gttgaacaga gccaggagaa cgcgtaagac tcaaggagga gcgtgtcatg
     4201 ctagtcctga gcaggaagct cggccagagt ttccacattg gtcctgaaat tcgcgtcacc
     4261 atcatcaaga tcgaccgcaa ctcggtgcgg atcggcattg aagcacccga cgaagtgtca
     4321 gtccagcgtg aggagatcgc cttcgaggtg ccagagcctc gcttcctgaa agtcggagcg
     4381 gcttgattcg gccggcggtc ccggcattac acgaagagtt gcccgattgg accgggttgc
     4441 gtggattgat tatgcttgca tcgacaagcc tgatccactg aataagacgc gcttcatgtc
     4501 gaagcagagc gtgagtggtc ggcgagcctt tgagcggggc cgacggcagt gcgcgtggtg
     4561 attgccccgg acaaattcaa ggggagcttg acggccctcg aggcggcggc cgcgatggcc
     4621 gttggtgtgg cgcgcgcggc acccgaggcg tcgatcgacc aggtaccaat ggctgacggc
     4681 ggtgagggaa ccacccaggc ccttgtcgcc gccaccggag ggtcattccg cgaggcgcag
     4741 gttaccggac ctttggcgga gccgatcatc gcctgtttcg gtctgctggg tgacggccgg
     4801 accgccgtga tcgagatggc ggccgcatca gggctcgtgc tcgtaccgcc cgaaaagcgc
     4861 aatccgctgg tcgcgaccac gcggggcacc ggcgagctgc tgctggcggc catcgccgcg
     4921 ggagcagggc gcgtagtgat cggcatcggc ggcagtgcga ccaacgatgg cggagccggg
     4981 ttcggacagg ccatcggctt ccggttgctc gacgaccaag atcgtgacct ccaacccggc
     5041 ggtggcaacc tggcccgcct ggcgcgcatc gatcgtttag cgcgccgtag tgagctcgaa
     5101 ggcgtcgaga tcgccgtggc ctgcgatgtc accaacaccc tgtgcggccc gcgcggagcc
     5161 tccgccgtct atggtcccca aaagggtgct aatccgtcga tggtcgaaat actcgaccaa
     5221 aacctggcgc atttcgcggc cgtcgtggaa cgcgacctgg gtgtggcaat caaagactta
     5281 cccggctccg gcgccgccgg cggactgggg ggcggcctgg tcgcctttgc cggcggcaag
     5341 ctcgagcccg gggtcgagct gatcgtcgac gccgtcaagc tcgccgatcg actcgaaagc
     5401 gctgacctct gcctgaccgg cgaaggggcg atcgatgccc agagcgcctt tggcaagacg
     5461 gcggtcggtg tcgcccagtt agcgcgatcg cttcactgtc ccacgctggc cctggccggc
     5521 tcgatcggtc caggcgccag ggccgttctc gagcacggaa tcg
//
LOCUS       DATAQL010001309         7162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_746658, whole genome shotgun sequence.
ACCESSION   DATAQL010001309 DATAQL010000000
VERSION     DATAQL010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746658"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="VHS97_22575"
     CDS             <1..1636
                     /locus_tag="VHS97_22575"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3451057.1"
                     /translation="MEVLCYDPIGQGERYQFLDAQGNPLVRGTTEHTMAGIGAILVGR
                     QLASYRIWDGLRALDYLASRPEIDPARLACTGNAGGGTMTSYLMALDERIAVAAPSCF
                     ITSLERLFATIGPQDAEQNITGQVAAGLEHADYITMRAPKPTLMTVGTRDFFDIQGSW
                     NSFREVKLIYGRFGFGERVDLFESDEPHGFTRPRRVATARWMRRWLLNKDDAVDEPDF
                     PIAADTELQCTRTGQVMSDFREISVFDLNRQRDHALRPSREAMSANALPSFRSEVKKR
                     LALDTGGLKTVRPTIGDTGQVRTLSFEVEPGLNLPALEVLSDRDASQGGPMVVKVGVD
                     WDKELAVLKGAEGRSGVGGNVVLFDPRGMGKTTPKAESGRGGSPFGRDWREAYVALHI
                     GRPLLGQRVADLISILAGLAAVSGAHGHNGFHVVGVGGAGPVVLHAALLDEHGLIKKV
                     TLERSLVSWSDIVERGVSRNQMGNVVPGVLESYDLPDLAARLAPLPLTIDSAVDAMGE
                     PVSQATLERSYARCAEAYGSGGKLILRANQGLSPSR"
     gene            complement(1612..4008)
                     /locus_tag="VHS97_22580"
     CDS             complement(1612..4008)
                     /locus_tag="VHS97_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451058.1"
                     /translation="MSPKSRARLTPIALSGLLITISTAAAQERMADDELSRHLTTVRS
                     QIENTSLNLSRREELALEMAGTLDRAAQSSTDPETRRRHWSEAIDLLDWFLKQHPDLP
                     RERQLRFQAGLYRWAQARSWTDTAVFDLNDPRPREQGAQALDNAIERFRAVAGDGNSP
                     TLADNLRFRLAEALADRADLYPAASSDRRTREREALDLLNQPPTEVGLAGYWHLLKAD
                     LLRRSAKPAEAEKEIAAAVKSTPPPPPREVVEVNVPLLLEQRKIADAIKALDSSKIDK
                     ATVALWMARIRLTERIGLPAGAERFKVDTDLFHWVNELRGGNTPERRQVLLDLARAEV
                     QPDARQPAEVWDALADAYGTAGEPAKAGAEMAKAAEHAAELGQTAAAMAYRLRGGGFL
                     LRAARFIEADALLSKVADDPSAGSLRAKAGMLRCLARGQALELGLQGSSSASYKAALE
                     QQLRDFPTDPSTDEARWLLGQLAVAAGDRARAETLWSAIAAVSPRWLDSRLAIAALDR
                     DELDRTLINPDRHQMQIIYQQADHFLDETIRRTRSEGALAELLLARARLELTPIVGRP
                     ESARELCERVARLAVGPGLRYRARLYRLVALVETSRYVEAEREAQTHSSWQVRTEHDA
                     FFDSVRLLDHGAAFAESDLRQRRFGLVLRLILEPLVAGDEKTDTDGRSELAMRYTRAL
                     LAVGADREARRSLPAWRGGPQSTDERVLRDLGDTYHRLGAHSLNIDVQRLRLKNNPSG
                     SLTWLDARYALALAYFNTGRAKDAAQLIDSTAILHPDLGGNALHDKFVHLRQRLGLKP
                     "
     gene            complement(3998..5305)
                     /locus_tag="VHS97_22585"
     CDS             complement(3998..5305)
                     /locus_tag="VHS97_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3451059.1"
                     /translation="MNSIRAKLSVMMFLELVIYGSWLPLLGLYIGNEYLKFTDDQQGW
                     VFNAFAIASITGMFFGGQLADRYFAQERFLGFSHLIGGLAMLGLAYQKTFWPFFGLML
                     LHCFFYVPTMSVTNAIAFANIKDARDFGFIRLWGTVGWIAASWPLIVIPIDWAKVPSI
                     EQAGGVIAWLGKALGTLKTGPGMEAALATTFTAAGIASLVLAVFCLFLPHTPPSKKAS
                     STFAPLEAFKLLAVPAILVLFIVTLFDSLVHYTYFYWASRYLRTIGPPENWISPSMCI
                     GQFAEIPAMAALGLALKRLGWRITMILGILGHVVRFGIYSIGNNDLLWLVILSNVMHG
                     FAYAFFFATVYIYVDENFPTDVRTSAQSLFNLLILGIGPLLSNFLSARLGVIFKGPDG
                     NVNYHQLFLVPLGLGLLATMILAIFFHPKAKDPVPEEVPVLVT"
     gene            complement(5410..6705)
                     /locus_tag="VHS97_22590"
     CDS             complement(5410..6705)
                     /locus_tag="VHS97_22590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451060.1"
                     /translation="MSSATMPKGPSGTNPLDSQQSGLAFAVADVGWFNTENLFREIDR
                     ESVEVLLLKCVDWVNGWRKGYYPWSRDCRLRRSSDTLWEQQFVLPSGWMKRFPRLGMR
                     PIARSIRRWWRMQPDTWRRGLVMSYPYYLYLHDQLRPDVSLYYNIDDYALYWPGAAEE
                     IRRLERAAICAADATVCVSKLRADELRALIPEAAGRIHHVPHGAPTSFLARHPLTQPG
                     RAPADLAHLPRPYLGYIGSLENRVDWELMNCISTTFSDVSIVVVGRVRNPVDEPWWNE
                     CARFLSQRNVHAIGWRSQEALADYYQAFDVTLIPYLMEHPFNRVCNPTKIMDAMGSGR
                     PIVATALPECRLHAERFHVAENRDEFVEAIRTILRQGSDDGRAGLRHAYALSNTCHEI
                     GERVLDLIDSAAGSNRPESGDPLALIRTCGTDTSSLRSR"
BASE COUNT         1412 a         2196 c         2255 g         1299 t
ORIGIN      
        1 gatggaggtt ctgtgttatg acccgatcgg gcagggggag cgataccagt ttcttgatgc
       61 gcaaggcaat ccgttggtgc gtggcaccac ggagcacacc atggcaggga tcggagcgat
      121 actggtcggc cgccagttgg caagctaccg gatctgggac ggattgcggg cgctcgacta
      181 cctggccagc aggcccgaaa ttgatcccgc tcgtctggcc tgcacgggta acgcgggcgg
      241 cggcacgatg acctcatacc tgatggctct cgacgagcgg atcgccgtgg cggcgccgtc
      301 gtgctttatt acctcgctcg agcggctctt cgccacgatc ggcccgcagg atgccgagca
      361 aaacattact ggtcaggttg ccgcgggtct ggaacacgcg gactatatca cgatgcgggc
      421 gcccaagccg actctcatga ccgtcggcac ccgcgacttc tttgacatcc agggaagctg
      481 gaattcgttt cgagaagtca aattgatcta cggccggttc gggttcggcg agcgggtcga
      541 cctttttgaa tcggacgagc cgcacggatt cacccgcccc aggcgcgtgg ccacggcccg
      601 atggatgaga cgctggctct tgaacaaaga cgatgcggtc gatgagcccg atttcccgat
      661 cgcggccgac accgagctac agtgcactcg gaccggtcag gtgatgagcg atttcagaga
      721 gatctcggtg tttgacctca accgccagcg cgatcatgca cttcgtccct cgcgcgaagc
      781 catgagtgcc aacgccctcc catccttccg atccgaggtc aagaagcggc tggctcttga
      841 caccggtggg ctcaagaccg tacggcccac tattggcgac accggccagg tgcgcacgct
      901 ctctttcgag gtcgagccag gcctgaactt gcccgctctc gaggtccttt cagatcgcga
      961 cgcgagccaa ggcggcccga tggtcgtcaa ggttggagtc gactgggaca aagagctggc
     1021 tgttctgaag ggagccgagg ggcgctcggg tgtcggcggg aatgtcgtgt tgttcgaccc
     1081 caggggaatg ggcaagacga cccccaaggc cgaatccggc cgcgggggtt ctccgttcgg
     1141 ccgcgattgg agagaagcgt atgtggccct ccacatcggc cggccacttc tcggtcagcg
     1201 cgtcgccgat ctcatctcga tcctcgcggg gctcgccgcc gtatcgggcg ctcatggtca
     1261 caacggtttc cacgtagtgg gcgtcggggg agccgggccc gtggtgctcc acgccgcctt
     1321 gctcgacgag cacggcctga tcaagaaagt cacgctcgag cgttcgctcg tatcgtggtc
     1381 cgacatcgtc gagcggggag tcagtcgcaa ccagatgggc aacgtggtcc ccggggtgct
     1441 cgaatcctac gacctgccgg acctggcggc gcgactggct ccgctgcccc tgacgatcga
     1501 ttccgccgtc gacgccatgg gcgagcccgt ttcccaggcc acgctcgagc ggtcttatgc
     1561 tcgctgtgcc gaagcgtatg gttcgggcgg aaaactgatt ctacgcgcga atcagggttt
     1621 gagcccgagc cgctgacgga ggtggacaaa cttgtcgtgg agtgcgtttc cccccaggtc
     1681 cgggtggagg atcgccgtcg agtcgatgag ctgagcagcg tccttggccc gtccggtgtt
     1741 gaagtacgcc agggccagcg cgtagcgtgc gtcgagccag gtgagggatc ctgacgggtt
     1801 gtttttgaga cgcagacgct ggacgtcgat gttgagcgag tgagcgccga gtcgatgata
     1861 cgtatcgccc aggtctcgga gaacgcgctc gtcggtcgat tgcggacctc cgcgccaggc
     1921 ggggagcgac cggcgggcct cacggtcggc gccgaccgcc agtagtgccc gagtgtaccg
     1981 catcgccagc tcgctacgcc cgtcggtgtc tgtcttttcg tcaccggcca cgagcggctc
     2041 caagatcaac ctgagtacga gcccaaaacg ccgctgccgc aagtcactct cggcgaaggc
     2101 ggcgccatgg tccagcaggc gaaccgagtc aaagaatgcg tcgtgttccg tgcgtacctg
     2161 ccacgacgaa tgggtctgtg cttcacgttc ggcctcgacg taccggctcg tctcgaccag
     2221 ggcaacgagg cggtagagcc gggcccggta ccggagaccg gggccgacgg cgagccgggc
     2281 aacacgttcg caaagctccc gggcgctttc gggcctgccc acgatgggag tgagctcgag
     2341 ccgggcacgg gccaagagca gctcggccag agcgccttcg gaacgggtac ggcggatcgt
     2401 ttcgtccagg aagtggtcgg cttgctgata gattatctgc atctggtgcc ggtctgggtt
     2461 gattagcgtg cggtcgagct cgtcgcgatc gagagcggcg atggccagcc gggaatcgag
     2521 ccagcgcggg gatacggcgg cgatcgccga ccacaaggtc tcggccctgg ctctgtcgcc
     2581 ggcggcaacg gcgagctgac ccagcagcca gcgtgcttca tcggtcgacg ggtcggtcgg
     2641 gaaatcgcgt agttgttgtt cgagcgccgc tttgtacgat gccgacgacg acccctgtag
     2701 acccagttcc agagcctggc ctcgcgccag gcagcggagc atacctgcct tggctcgcag
     2761 ggagccggcg cttgggtcgt cggcaacctt cgacaggagt gcgtcggctt cgatgaaacg
     2821 agcggcccgc aacagaaagc cgccgccgcg cagtcgataa gccatcgctg cggcggtctg
     2881 ccccagctcg gccgcatgtt cggctgcttt tgccatttcg gcgccggcct tggctggctc
     2941 acccgccgtc ccgtaagcat cagccaacgc atcccagact tcggcaggtt gcctggcatc
     3001 gggctggact tccgcccgcg ccagatccag gagcacctgg cggcgctccg gagtattccc
     3061 accccgcagc tcgttgaccc agtggaacaa gtcggtatcg accttgaaac gctctgcacc
     3121 ggccggtaat ccaattcgct cggtcaggcg aatccgcgcc atccagagtg ccacggtcgc
     3181 cttgtcgatc ttggacgaat cgagcgcctt gatcgcgtcg gcaatctttc gctgctccag
     3241 gaggagcggg acattcacct cgacgacctc tcgcggcggt ggaggggggg tcgatttgac
     3301 cgcggcggca atttccttct cagcctcggc gggtttcgcc gaacggcgca acagatcggc
     3361 cttgagcaag tgccagtaac cggcaagacc cacttccgtc ggaggctgat tcagcaggtc
     3421 gagggcctcg cgctctctcg ttcgccgatc actcgatgcg gccggataaa ggtccgcgcg
     3481 atcggccagt gcctcggcaa ggcgaaaccg cagattgtcg gccagggtcg ggctatttcc
     3541 gtcgccggca accgcgcgaa accgctcgat cgcgttgtcg agggcctgcg caccctgctc
     3601 gcgcggtcgt ggatcgttca ggtcgaagac cgccgtgtcg gtccagctcc gcgcctgcgc
     3661 ccagcgataa agtccggctt gaaaccgaag ctgccgctcg cgcggcagat cggggtgttg
     3721 tttcaggaac caatcgagca ggtcgatggc ttcggaccag tgccgtcgcc gcgtctccgg
     3781 atcggtagac gattgagctg cgcggtccag cgtgcctgcc atttcgagcg ccagctcttc
     3841 acggcgcgac aaattgagcg acgtgttttc gatctggctt cgaaccgtcg tcaagtgccg
     3901 cgacagctcg tcgtcagcca tgcgttcctg cgcggccgcc gtcgatatcg taatcaatag
     3961 acccgagagg gcaatcggcg tcagccgcgc ccgcgactta ggtgacaagt acgggaacct
     4021 cttcgggaac cgggtctttc gccttggggt gaaagaagat cgccaggatc atcgtcgcca
     4081 ggagccccag accaagcggt acgaggaaca gctggtgata attgacgttg ccgtcgggcc
     4141 ctttgaatat tacgcccagc cgggccgaga ggaaattgct gagaagggga ccaatcccaa
     4201 ggatcaacaa gttgaaaaga ctctgcgcac tggttcgtac gtccgtgggg aagttttcgt
     4261 ccacgtaaat atacaccgtc gcgaagaaga acgcataggc aaatccatgc atcacgttgc
     4321 tgaggatgac caaccagagc aagtcgttgt tgccgatcga atagatccca aagcggacca
     4381 cgtggccgag gatgccgaga atcatggtta tccgccaacc gagtcgtttg agcgccaagc
     4441 ccaaagcggc cattgccggg atctcggcga actgaccgat acacatcgac ggcgagatcc
     4501 agttttcggg agggccgatg gttctcaggt agcgacttgc ccaatagaag taagtgtagt
     4561 gcacgagcga gtcaaacaac gtcacgatga aaagcaccag gatggcggga accgccaaga
     4621 gcttgaacgc ctccaggggc gcgaaggtgg aactggcttt cttcgacggc ggggtatggg
     4681 gaaggaacag acagaacacg gcgagtacga gcgaggcgat cccggcggcc gtgaacgtcg
     4741 tggccagcgc cgcctccatt cccggaccgg tcttcagggt gccgagagcc ttacccagcc
     4801 atgcgatgac gccacccgcc tgctcaatgg acggaacttt cgcccagtca atgggaatga
     4861 cgatcaacgg ccaactcgcc gcgatccagc cgaccgtccc ccacaacctg atgaaaccaa
     4921 agtccctcgc gtccttgata ttcgcaaagg cgattgcgtt ggtcaccgac atcgtgggga
     4981 cgtaaaagaa gcaatgcaag agcatcaggc caaagaacgg ccagaacgtc ttctgatagg
     5041 ccaggccaag catggccagc ccgccaatca aatgactgaa accgagaaac cgctcctgcg
     5101 caaaatagcg gtcggcgagc tgaccgccga agaacatgcc agtaatcgac gcgatggcga
     5161 atgcgttgaa aacccagccc tgctggtcgt ctgtgaattt caagtactcg ttaccgatgt
     5221 ataaacccaa gagcggtagc caggagccat agatcacgag ctccagaaac atcattacgg
     5281 atagcttcgc acgaatcgaa ttcatctggt cgctcgccgg tctaggttgg tggaaattag
     5341 ttgatccctg gcctaggcgc cggccccagc caggatttga atgaatctac cgcttggcgg
     5401 ctcgagttgc tatctgcttc tgagcgaaga tgtatcagtt ccgcaggttc tgatcagagc
     5461 aagaggatcg cccgactcgg gccgattcga accagccgcg gaatcgatca gatccaggac
     5521 gcgctcgccg atctcatggc aggtgttcga aagggcatac gcgtgccgca acccggcgcg
     5581 gccatcgtca gatccctggc gaaggatcgt tcggattgcc tcgacaaact cgtcgcgatt
     5641 ctcggcgacg tgaaaccgct cggcgtgcag gcgacattcg ggcagcgcgg tcgccacgat
     5701 cggccgaccc gaccccatcg catccatgat cttcgtcggg ttgcacacac ggttgaacgg
     5761 gtgttccatc aagtacggga tgagcgtcac atcaaaagct tggtagtagt cggcgagagc
     5821 ctcctgggat cgccagccga tcgcatgcac gttgcgctga gacaaaaatc gcgcgcattc
     5881 gttccaccac ggctcgtcga ctgggttgcg tacccggccg accaccacaa tcgagacgtc
     5941 gctaaacgtg gtactgatac aattcatcag ctcccaatcc acgcggtttt cgagtgagcc
     6001 gatgtaaccg agataagggc gcggcagatg cgctaaatcg gcaggcgcgc ggcctggctg
     6061 cgtgagcggg tgccgggcca agaacgatgt gggagcgccg tgcggaacgt ggtggatgcg
     6121 cccggccgct tccggtatga gcgcacgcag ctcatcggcc cgcagctttg acacgcaaac
     6181 agtcgcgtcg gccgcgcaaa tggccgcccg ctcgagcctg cggatctctt cggccgcgcc
     6241 gggccagtac aaggcgtaat cgtcgatgtt gtagtagagg gatacgtcag gacgaagctg
     6301 gtcatgcagg taaagatagt aagggtacga catcaccaat ccccgccgcc atgtgtcggg
     6361 ctgcatgcgc caccaccggc gaattgatct ggcgataggg cgcatgccca gccgcggaaa
     6421 acgcttcatc cagccgctcg gtaagacgaa ctgctgctcc cacaacgtgt cgctagagcg
     6481 tcgcagccgg caatcgcgcg accagggata gtaccccttc ctccaaccgt tgacccaatc
     6541 gacgcacttg agcagcaata cctcgaccga ttcgcgatcg atttcacgga acaggttctc
     6601 agtgttgaac cagccgacat ccgcgacggc aaacgccaga ccggattgct gcgagtcaag
     6661 cggattcgtc cctgatgggc ctttaggcat cgtcgcgctg ctcatcctcg ctgcgcccca
     6721 acgccactct ccgagccgtc gttcacggac gcaacgccaa ctcggacgat gtggcggaat
     6781 tatttacggg atccgatcta acatcaaatg tataacccag ttccacccgc ccgtcaacgc
     6841 ggcgttcccc gcgaagcagg tagtcgccga agtccagcga gggccggtcg agcgtacgcg
     6901 gaaccattgc ctcttcgcgt tggcccgtcg ggatcggggt tggtttggcc ctccgcctgg
     6961 cagcgaaatc cgccttcttc gtaactgaag ttcacatccc atcgttgctt tcaagagaga
     7021 caagatcgaa gcaactcggc gggatgaatc gcttcgcggc aggtgaaatg ctcgatctgc
     7081 agccggcacg agaacacggg ggcaaccacg acagtgtctg gcgtagcgtg actgattgcg
     7141 ggtaagagac gctgttcacc ca
//
LOCUS       DATAQL010001310         7561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3492485, whole genome shotgun sequence.
ACCESSION   DATAQL010001310 DATAQL010000000
VERSION     DATAQL010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3492485"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VHS97_22595"
     CDS             <1..1143
                     /locus_tag="VHS97_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3451061.1"
                     /translation="EHDLVAVAVLSGNRNFEGRINPDVRANYLASPPLVVAYALAGTM
                     DIDLQTEPLGSDRQGKPVYLKDIWPSQREIADTILKSVRSEMFQTKYAEVFEGDKRWR
                     SLPVPQGDLYSWDPKSTYVKNPPYFLDMKIDPPPVAPIEKARVLAVLGDSITTDHISP
                     AGSIKPDSPAGRYLIEHGVGVADFNSYGARRGNDEVMVRGTFANIRLKNRLAPGTEGG
                     WTRHLPDGQQMTIFDASRRYLEEGVALIVIAGKEYGSGSSRDWAAKGPLLLGIRAVIA
                     ESYERIHRSNLVGMGILPLQFQPGQSAQSLSLTGEEIYSIEGLSPAIAGRFAGGRTLT
                     VVAEPVDAAPIRFEVSVRIDTPQEIRYYENGGILPYVLRQLLQEST"
     gene            1503..2522
                     /gene="pdhA"
                     /locus_tag="VHS97_22600"
     CDS             1503..2522
                     /gene="pdhA"
                     /locus_tag="VHS97_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX3451062.1"
                     /translation="MTMVQVQTEAKPIVNRTQAQVWLRQMMLIRRFEERAEMLYQKGN
                     KIGGFFHQYSGQEPVAVGSIGVLRENDYVITAYRDHGHALARGMSARAGMAELLGKAA
                     GCSKGKGGSMHFFDVEKGFLGGHAIVGSHIPLAAGVGFAIKYRGEDRVCVCYFGDGAV
                     NQGGVHEAFNMAALWKLPVIYIVENNSYAMGTSLARSSAVLDLTVRCAVPYGIPGYQI
                     NGNDVELMAKTTREAANRARAGEGPTFIDALTYRYKGHSISDPGKYRHKEELETAIRN
                     DPILVYQNVLRERGWIDQEAIDRMLESVKEEVEESIEFAEQCDPPAMETLYEDITVSP
                     FIPQE"
     gene            2525..3502
                     /locus_tag="VHS97_22605"
     CDS             2525..3502
                     /locus_tag="VHS97_22605"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX3451063.1"
                     /translation="MPVVSFREALNQAMTEEMERDENVFLMGEEVAEYDGAYKVSQGM
                     LKRFGPRRVVDTPISEEGFAGIGIGAAMVGLRPIIEFMTFSFSLVAIDQIVNNAANMR
                     YMSGGQFAVPIVFRGSSGMAGCLAATHSHRLEAWYAQIPGLTVILPGTPADAKGLLKS
                     AIRSDDPVVFIEHEVMYHEKGDVPEGEHLVPIGKADIKRPGSDVTLITYSRSLKATLA
                     AADKLAQEKIDAEVIDLRTIRPLDLDTLLTSVTKTHRAVIVEEDWPYCGLGAGIADRI
                     TQRVFDELDAPIRRVASKDAPIPYNKALENSMLPSVDRVIALVHDVLYI"
     gene            3550..4872
                     /locus_tag="VHS97_22610"
     CDS             3550..4872
                     /locus_tag="VHS97_22610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX3451064.1"
                     /translation="MPIEVTMPKLSPTMETGVIAHWLVKVGDQIKEGDSLADIETDKA
                     TMPMKSYDDGTIVHLDHAAGDEVALGQRVMVLAKKGEDPQKIVESMGLGKTPAAASGA
                     VARPNSAEESSAPSRSAGNGQQAPAVEAQPVGGRLKASPLARKVAASSQVDLGEVRGS
                     GPGGRIVRRDIESFLQGEPKARRTPTSAPSKPPAAAATTARLSSSQPSSIAPAGQRVP
                     HSRMRKTIASRMLQAKQAAPEIHLTIDARVDRLVSAREQLNKQLAGDGIKISLGDFVT
                     KAVALALRLHPGLNASFEPDAIVRHGEVNIGIAVALEEGLIVPVLHNADTLGLIDIRV
                     KSDALASAARGGTLTGEQLTGGTFTISNLWMFGTRQFDAILNLPEVGILAVGAAEKRP
                     VVVGDQLTVGTVMTLTLTADHRAVDGAMAARFLQTVKGLLEEPVSMLL"
     gene            4928..6313
                     /gene="lpdA"
                     /locus_tag="VHS97_22615"
     CDS             4928..6313
                     /gene="lpdA"
                     /locus_tag="VHS97_22615"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX3451065.1"
                     /translation="MAYDFDIVVIGGGPAGYAAAIRAAQLKKRTLCIEKDKLGGICLN
                     WGCIPTKALLTNAHFVELINNHGSHFGYSGAASWDFAQMIKRSRNVAGQLNKGIEGLF
                     RKYKVSSKFGVAKVITPHIVQVNGEAISAESIVIATGVRPRPLPGAEYDGKTIISYKE
                     AMSLATRPQSMLIIGGGPIGLEFGYFYNAIGTKVTVVELLDRILPGEDDDISAALEKS
                     LTKRGLTILTGSKTTKVDKTGKGIKAELETPKGKVTVEADLMLVAIGVLGNVEGLTAD
                     EVKLEIVKNHIKADPKNGYVTSVPGIYAVGDVIGPPWLAHVAHHEAVACIERLCGFAD
                     RPIDYTNIPGCTYTDPGVASVGLTEKAAKEAGHEVRIGKFPFNFSGRALASDETEGFV
                     KLVFDAQYGELLGAHLIGSQATEMIAELVLAKKLEATEEEILHAMHPHPTYSEAIMEA
                     AGQGMGESIHI"
     gene            complement(6320..7153)
                     /locus_tag="VHS97_22620"
     CDS             complement(6320..7153)
                     /locus_tag="VHS97_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451066.1"
                     /translation="MASFWSSVLRIEALWNSPLRVEVLRILATTIFFGLGIVAGRLWG
                     MWRRYRQLRIAERGESEDVVTIEKIILDRCPDGREVLRIRSCGRDPIETVFPNPAARD
                     EFQSRAGKTTTARPLVSMEGKMGSYLLQELAIWVCGQLRERNFRHDVWVMAPVYERGA
                     LYLGGHFLSTVLLIRLDDLFRFRDWDECAAIQVEHASHGERVLTLFGMAAEYDRQASA
                     IASRRAAGRRSSYEETMYILDMGLDTQTVDLATKTVPWERFESTLKQLGIAAREPVKA
                     A"
BASE COUNT         1573 a         2259 c         2330 g         1399 t
ORIGIN      
        1 gaacacgatc tggtcgccgt cgccgtcttg agtggcaacc gcaacttcga gggacggatc
       61 aatcccgatg tgcgggccaa ttacttggcc tcccctccct tggtggtggc ttatgccctg
      121 gccggcacaa tggacatcga ccttcagacc gagccgctgg gatccgatcg ccagggtaaa
      181 ccggtctatt tgaaggatat ctggccgagc cagcgcgaga ttgcggacac cattttgaag
      241 tcggtgcgct cggagatgtt tcaaacgaaa tatgccgaag tgttcgaggg agacaagcgc
      301 tggaggtcgt tacctgtgcc ccagggcgac ttgtactcgt gggacccgaa gtcgacgtac
      361 gtcaagaatc ctccgtattt cctcgacatg aagatcgacc cgccgcctgt cgcgccgatc
      421 gagaaggcgc gggtgttggc ggtcctgggc gacagcatca cgaccgacca catttctccg
      481 gccgggtcga tcaagccgga cagcccggcc ggccgctacc tgatcgaaca cggcgttggt
      541 gtcgccgatt tcaactctta tggcgcgcgg cgcggtaacg acgaggtgat ggtgcgagga
      601 actttcgcca acatccggct caagaaccgc ctggcacccg ggaccgaagg gggctggaca
      661 cggcatcttc ccgatggcca gcagatgacg atttttgacg cctcgcggcg ctacctggag
      721 gagggagtcg cgctgattgt aatcgccggc aaggaatacg gctcgggctc gtcgcgcgat
      781 tgggcggcca aagggccgct cttgttgggc atccgcgcgg tgattgccga gagctacgag
      841 cggatccatc ggtccaactt ggttggcatg ggaattctgc cgctccagtt tcagccaggc
      901 cagagcgcgc aaagtctctc tttgaccgga gaagagatct attcgatcga aggcctgtcc
      961 ccggcgattg ccggccgttt cgcggggggc cgtacgctga ccgtggtcgc cgaaccagtt
     1021 gatgctgctc cgattcgatt cgaggtgtct gtccggatcg acactcccca ggagatccgg
     1081 tactatgaaa atggcgggat tcttccgtac gtgcttcgcc aattgctcca ggagtcaacg
     1141 tgagcccgcc gactggtttc ctggccgttc cccgccgatt ctctagaggg cagccggatg
     1201 ccaacagcct aaggaattga gcagagcctc cctggaacgg cccgccgatc ttgtgtaagt
     1261 tattcccgac aaatactttg actcattcat accgcaagcc gtccccgcaa gaatcgatat
     1321 cgaggcatgg taagtttccg taactcccga tccggcatgg atcttcctgg cccgcctgcc
     1381 actggccgag ttgagcgagg cgttcatcac cgcgacgccg tctttcccgc tgacgcaacg
     1441 accgcgatgc cccgactcgt tttcatgccg ccggcccagt cccccatcca ctcaggagtt
     1501 ggatgactat ggtgcaagtg cagaccgaag cgaaaccgat cgtcaaccgc acccaggccc
     1561 aggtgtggct ccgccagatg atgctgatcc gccgattcga ggaacgggca gagatgctct
     1621 accagaaggg aaacaagatc ggcggctttt ttcaccagta tagcggccag gagccggtcg
     1681 cagtcggctc gatcggagtg ctccgtgaaa acgactatgt gatcacggcg tatcgcgacc
     1741 acggtcatgc gctggcgcgg gggatgagcg cgcgggccgg gatggccgag ctcctgggca
     1801 aggcggccgg ttgctccaag gggaaggggg gctcgatgca cttcttcgac gtggagaagg
     1861 gattcttggg gggccacgcg atcgtcggca gccatatccc cctggcggcg ggagtcggct
     1921 tcgcgatcaa gtaccggggc gaagaccggg tttgtgtctg ctacttcggc gatggcgcgg
     1981 tcaatcaagg tggggttcac gaagccttca acatggccgc actctggaag ctgccggtga
     2041 tctacatcgt cgagaacaat tcgtacgcca tgggcacctc gctggcccga tcgtcggcgg
     2101 tgctcgacct taccgttcgc tgcgccgtcc cgtatggaat tccgggctac cagatcaacg
     2161 gtaacgacgt ggagctgatg gccaagacga cgcgcgaggc tgccaaccgg gcccgggccg
     2221 gtgaggggcc cacattcatc gacgctttga cgtaccgcta caaggggcac tcgatcagcg
     2281 atcccggcaa gtatcgccac aaggaagagc tcgaaacggc gatccgtaac gacccgattc
     2341 tggtttatca aaacgtcttg agagaacgcg gctggatcga tcaggaggcc atcgatcgaa
     2401 tgctcgagag cgtcaaggaa gaagtggaag agagcatcga atttgccgag cagtgcgatc
     2461 cgcccgccat ggaaacgctc tacgaagata tcacggtgtc cccctttatc ccgcaggagt
     2521 gatcatgccg gttgtttcgt ttcgcgaggc cttgaaccag gcgatgaccg aggaaatgga
     2581 gcgcgacgaa aacgtcttcc tgatggggga agaagtcgcc gagtatgacg gagcgtacaa
     2641 ggtcagccag gggatgctca agcggttcgg tccgcggcgg gtggtcgaca ctccgatctc
     2701 ggaggaaggg ttcgccggca tcggaatcgg cgcggccatg gtgggcttac ggccgatcat
     2761 cgaattcatg actttcagct ttagcctggt ggcgatcgac cagatcgtca acaacgcggc
     2821 caatatgcgc tacatgagtg gtggccagtt tgcggtgccg attgtttttc gaggaagctc
     2881 gggcatggcc ggctgcctgg cggccacgca ctcgcaccga ctggaagcct ggtacgcaca
     2941 gatccccgga ttgaccgtca tcctgccggg gacaccggcc gacgccaagg gtctgctcaa
     3001 aagcgcgatc cgctccgatg atccggtcgt attcatcgag catgaagtga tgtaccacga
     3061 aaagggagac gtccccgagg gagaacacct ggtaccgatc ggtaaagccg acatcaagcg
     3121 tccgggctct gacgtcaccc tgatcactta ttcgcggtcc ttgaaggcaa cgctggccgc
     3181 cgccgacaaa ctcgcccaag aaaagatcga cgccgaggtg atcgacctgc ggaccattcg
     3241 tccgctcgac ctggatacgc tcctgacgtc ggtcaccaag acgcaccgcg ccgtgattgt
     3301 cgaggaagac tggccctatt gcgggctagg cgccggaatc gccgaccgga tcacccagcg
     3361 cgtctttgac gagctcgacg cgccgataag gcgcgtcgcg tcgaaagatg cgcccattcc
     3421 gtacaacaaa gcactcgaaa actcgatgct gccatcggtc gatcgagtca tcgcgctggt
     3481 gcatgatgta ttgtatattt gaatgcatct ggaagtcacc ggccggacca actggagggg
     3541 agaagagacg tgccgattga agttaccatg ccgaagctca gtccgacgat ggaaaccggc
     3601 gtgatcgcgc attggctggt caaggtaggc gaccagatca aggaagggga ttcactggcc
     3661 gatatcgaga ccgacaaggc aacgatgccg atgaagtcgt atgacgacgg gacgatcgtc
     3721 catctggacc atgccgccgg cgatgaggtc gcgctcgggc agcgggtgat ggttctggcc
     3781 aaaaagggcg aggatccgca aaaaattgtc gaatcgatgg gcctgggcaa gactccagcg
     3841 gcggcgagtg gagcagtcgc caggccaaat tccgcggagg aatcatcggc tcccagccgt
     3901 tccgcgggca acgggcagca ggcgcccgcc gtggaggcac aaccggtagg tggccggttg
     3961 aaggcgagtc cgctggcgcg caaagtggcg gcttcgtctc aggtggattt aggagaggtc
     4021 cggggctccg gtccaggcgg ccgtatcgtg cgccgcgaca tcgagtcatt tcttcaggga
     4081 gagcccaaag cgcgccgcac cccgacgtcc gccccgagca agccacccgc ggcggcagct
     4141 acgacggcaa ggttgtctag ttcacagcca tcgtcgatcg ctccggccgg ccagcgagtc
     4201 ccacattcgc gaatgcggaa gacaatagcc agccggatgc ttcaggccaa gcaggccgct
     4261 cccgagatcc atctgacgat cgatgcccgc gtcgaccggc ttgtatcggc gcgagaacaa
     4321 ctcaacaaac aactcgccgg cgatgggatc aagatttctc tgggcgactt tgtcaccaag
     4381 gcagtcgcgc tggcgctgcg gcttcatcct ggcctgaacg ccagtttcga gccggacgcg
     4441 atcgttcgtc acggcgaggt caacatcggg atcgccgtcg cactcgaaga gggcttgatc
     4501 gttccggtgc tgcacaatgc cgatacgctg ggattgatcg atatccgtgt caaaagtgat
     4561 gccctggcgt cggcggcgcg ggggggcacg ctcacgggcg agcaactgac cggcggcacc
     4621 tttacgatct ccaacctgtg gatgttcggg acgcgacagt tcgatgcgat tctcaacttg
     4681 cctgaggtcg gtatacttgc agtgggtgcc gcggaaaagc gaccggtcgt cgtgggagac
     4741 caactgaccg tcggcaccgt gatgactctc accttgacag ccgaccatcg cgcggtcgac
     4801 ggggccatgg ccgcccgatt cctccagacc gtaaaggggc tcctggaaga accggtgtcc
     4861 atgctgctct aggcgacgcc atcgccccca ctctgttgca atacctccgg gacgagaaaa
     4921 gagaatcatg gcctacgatt ttgacatcgt cgtcatcggc ggcgggcccg ccggttacgc
     4981 cgccgcaatt cgcgccgccc agctcaaaaa acgcacactc tgcatcgaga aagacaagct
     5041 cggtgggatc tgtctcaact ggggatgcat tccgaccaag gccctcttga ccaacgccca
     5101 ctttgtcgag ctgatcaata atcacggcag ccatttcggc tatagcggcg ccgccagctg
     5161 ggacttcgcc cagatgatca agcgcagtcg caacgtggca ggtcagttga acaaggggat
     5221 cgaagggctc ttccggaaat acaaggtaag ctccaagttt ggcgtcgcca aggtgatcac
     5281 gcctcatatc gtccaggtaa acggcgaagc gatctcggcc gagtcgatcg tgatcgcgac
     5341 gggagtgcga ccgcgtccgc tgcctggtgc cgagtacgac ggcaagacga tcatctcgta
     5401 caaggaagcg atgtcgttgg cgacccgtcc ccaatcgatg ctcatcatcg gcggcgggcc
     5461 gatcggcctg gaattcggct atttctacaa tgcgatcggc accaaggtga ccgtcgtcga
     5521 gctcctcgac cggatcttgc ccggcgagga cgacgacatc tccgcggctc tggaaaagag
     5581 cctgaccaag cgaggactga cgatcctgac gggttccaaa acgaccaagg tcgacaagac
     5641 cggcaagggg atcaaggctg agctcgagac accgaaggga aaggttactg tcgaggctga
     5701 cctgatgctc gtcgccatcg gagtgcttgg caatgtcgag gggctgacgg ccgacgaggt
     5761 caagctcgag attgtgaaga accacatcaa ggccgacccc aaaaacggct acgtcacgag
     5821 cgttccgggc atctatgccg tcggcgacgt gattggcccg ccgtggctgg cccatgtggc
     5881 ccatcacgaa gcggtcgctt gtatcgagcg attatgcggt tttgccgacc ggccgatcga
     5941 ctatacaaac atccccggtt gtacctatac tgatccgggc gtggccagcg tgggcttgac
     6001 agagaaagcc gccaaggagg ccggccacga ggtgcgcatt ggcaagtttc ctttcaactt
     6061 tagcggccgg gctctggcgt cggacgagac cgaggggttc gtgaagcttg tctttgacgc
     6121 ccagtacggc gaactcttgg gtgcccacct gatcggttct caggccaccg agatgatcgc
     6181 cgagctggtg ctcgccaaaa agttggaagc cactgaggag gaaatcctcc acgccatgca
     6241 tccccacccg acgtattccg aggcgatcat ggaagccgcc gggcagggga tgggggaatc
     6301 gatccatatc taaccaggtt caggctgcct tcacaggttc tctcgccgcg attcccagtt
     6361 gtttgagcgt tgattcaaac cgctcccagg ggacagtctt ggtcgcaagg tccaccgttt
     6421 gcgtatcgag ccccatgtcg aggatataca tcgtttcttc ataactggat ctccggcctg
     6481 ccgcccggcg gctggcgatc gcggaggctt gccggtcgta ttcggcggcc atgccgaaga
     6541 gcgttagcac gcgttcgcca tggctggcgt gttcgacctg gatcgccgca cattcgtccc
     6601 agtcgcgaaa ccgaaacagg tcatccagcc ggatcaaaag tacggtcgac aggaagtgac
     6661 cgcccagata aagcgcccct cgctcgtaca ccggagccat cacccagaca tcgtggcgga
     6721 agttgcgctc gcgaagctgg ccgcaaaccc agatcgccag ttcctgcaac aaataggacc
     6781 ccatttttcc ttccatcgat accagggggc gagcggtcgt cgtcttgccg gcgcgcgact
     6841 gaaactcgtc gcgggccgcc gggttgggaa agaccgtctc gatgggatcg cggccgcagg
     6901 accggatgcg caatacctct cgtccgtcgg ggcagcgatc gagaatgatc ttctcgatcg
     6961 taacgacatc ttctgattca ccacgctcgg cgatccggag ctgacgataa cgccgccaca
     7021 tgccccagag cctccccgcc acaataccca acccaaagaa aatcgtcgtc gccaggatcc
     7081 gcaggacctc aacccgcaag ggggagttcc acagagcctc aatgcgcaag accgagctcc
     7141 aaaagcttgc cacgttcgtc gtcctttact caataaaagg cgcggtctgc ctactggcga
     7201 tgtgcgccgc gtccaaaaac cttctgacca tgtctctcgg gacatagggt cgggcattct
     7261 gatccgcgcc ttaatcgaag cttgtcccac gtaaaacggt cgaatggacg cgcatgtcta
     7321 ttcgacaaat cttcgacgat cttggggagt gagcgacggc ggccagaccg aattcgacgc
     7381 agtcgctata tcggcttaca agcccgagac ttacacaact cgcaggggat ccagcgcccg
     7441 cggcaagcga cgcgcagtgc agtatggccg gtgccgacgt cgccatttca tgctgagctg
     7501 cccagcttgg aacacgtgtt ccgccgtggc aatagcaacc ggtttcttga actcggcggg
     7561 g
//
LOCUS       DATAQL010001311         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1052366, whole genome shotgun sequence.
ACCESSION   DATAQL010001311 DATAQL010000000
VERSION     DATAQL010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052366"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VHS97_22625"
     CDS             <1..1351
                     /locus_tag="VHS97_22625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451067.1"
                     /translation="ALNMRLVGSNDLQGRSAYQPTIAHQGDRYIAYIGHHGGTPEVPK
                     PMNSLSGQAEFNGTSIVDVTDPAHPKYLKHIPGLEGNYEDGGGQMTRVCPGRLLPKAD
                     PAKYYLLRVFGGQGHEIWDVTDPVNPTLLVRLMGLKDTHKSWWECDTGIAYLVSGVPG
                     WRVRRMTEVYDLSDPTKPVKIRDFGLPGQEPGATGTVPTELHGMISLGPQSNRIYFGY
                     GTNKGGILQIVDRDKLLRGPKEPTPENLRYPEVGRLEMTPMNGAHTVFPMPKMAVPEF
                     AGDKAGSTRDFVMIVDESLVNECQEAETRQMVWFADITIETKPMVVSNYTVPEKSGDF
                     CHRGGRFGSHSSNESMAPVFYKKVAFVTWFNAGVRAIDVRDPYRPKEIGYFIPAITEA
                     TDKRCIKVNGQDQCKVAIQSNNVETDDRGYVYVVDRANTGLSILELTPEGKKAAGLE"
     gene            complement(1505..>1778)
                     /locus_tag="VHS97_22630"
     CDS             complement(1505..>1778)
                     /locus_tag="VHS97_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451068.1"
                     /translation="RELNFNVYADNRALFRYDMHTLPSEVSGSIGTSTVWAAWNAAVY
                     VAQVEDERLAHVANDTRYIEATTEVLHKNGGLWFYDESYVISRRRE"
BASE COUNT          376 a          538 c          540 g          324 t
ORIGIN      
        1 agcgctcaac atgcggctcg ttggcagcaa cgatctgcaa gggcgcagcg cctaccaacc
       61 gaccatcgcc catcagggcg atcgctacat cgcctatatc ggtcaccatg gcgggacgcc
      121 ggaggtgccc aaaccgatga actcgttgag cgggcaggca gagttcaacg gcacgtcgat
      181 cgtggacgtg actgatcctg cccatcccaa gtacctcaag cacattcccg ggctcgaagg
      241 gaattatgag gacggcggcg ggcagatgac gcgtgtctgt ccgggccggt tactgcccaa
      301 ggcagatccc gctaagtatt acttgctgcg cgtgttcggt gggcaggggc acgagatctg
      361 ggacgtcacc gatccggtga acccgactct gctcgtgcgg ctgatgggcc tcaaagacac
      421 gcataagagc tggtgggaat gcgacaccgg cattgcctac ctggtctccg gtgtacccgg
      481 atggcgcgtg cgtcgcatga ccgaggtcta tgatctcagc gatccgacca agccggtgaa
      541 gatccgcgat ttcggacttc ccggccagga gccgggagcc accggaacgg tgccgaccga
      601 gctgcacggc atgatctcat tgggtccgca gtcgaaccgg atctacttcg gctacggcac
      661 caacaagggc ggcatcctgc agatcgtcga tcgcgataag ctcttgcgcg gccccaagga
      721 gccgaccccg gaaaacctgc gctatccgga agtggggcgc ctcgagatga cgccgatgaa
      781 tggcgcccat accgtcttcc cgatgccgaa gatggcagtt cctgaattcg cgggcgataa
      841 ggcgggatcg acgcgcgact tcgtcatgat cgtcgacgaa agtctggtca acgaatgtca
      901 ggaagccgag acccggcaga tggtgtggtt tgccgacatc accatcgaga ccaagccgat
      961 ggtcgtttcc aactatacgg tcccggaaaa gagcggtgat ttctgtcatc gcggcggccg
     1021 cttcgggtca cactcgagca acgagagcat ggcgccggtc ttctacaaga aggtcgcgtt
     1081 cgtcacctgg ttcaacgccg gcgttcgtgc catcgatgtg cgcgatccgt accggccgaa
     1141 ggaaatcggc tacttcatcc cggccattac ggaagcgacc gacaagcgct gcatcaaggt
     1201 caatggccag gatcagtgca aagtcgctat ccaaagcaac aacgtggaga ccgacgatcg
     1261 cggttatgtc tatgttgttg accgcgccaa cactggtctc tcgattctcg aactcacgcc
     1321 cgagggcaag aaggccgcgg ggttggagta gcgcgacaaa cttccagcct ctcggttcgt
     1381 catggccggc cttgtgccgg ccatctcgca taggaaagca cggtgcgtcc ctaatcgaga
     1441 tcgccgggac aagcccggcg atgacgagga gagtgggtca attgacgaga agacgctttg
     1501 ttgatcactc ccgccggcgc gagatcacat aggactcgtc atagaaccac agcccgccgt
     1561 tcttatgcag cacctcggtc gtggcctcga tgtagcgagt atcgttggcg acgtgtgcca
     1621 gccgctcgtc ttcgacctgc gccacgtaga ccgccgcgtt ccacgcggcc cacaccgtcg
     1681 acgttccgat cgagcctgag acctccgacg gcaacgtgtg catgtcataa cgaaacagcg
     1741 cgcgattatc ggcatagacg ttgaaattca gctcacgg
//
LOCUS       DATAQL010001312         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2428127, whole genome shotgun sequence.
ACCESSION   DATAQL010001312 DATAQL010000000
VERSION     DATAQL010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428127"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VHS97_22635"
     CDS             complement(<1..193)
                     /locus_tag="VHS97_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3451069.1"
                     /translation="MLLELTGISKAFAGVQALRSVSFDLCAGEVHALVGENGAGKSTL
                     IKVITGAHLPDLGTIEVMGR"
     gene            complement(223..1536)
                     /locus_tag="VHS97_22640"
     CDS             complement(223..1536)
                     /locus_tag="VHS97_22640"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEX3451070.1"
                     /translation="MPILTIPALVRDGDMASPGENVVRAFAGAKEQYAEWGVDVEVAL
                     ERLSAIAISLHCWQGDDVAGFENSSQELGGGLAVTGHYPGKARTPDELRADLDKTLSL
                     LPGSHRLNLHASYADTKGRKIERDQLQPEHFETWIHWAQSRRIGMDFNPTYFAHPLAA
                     DGYTLAHPDRSIRRFWIDHGIACRQIGAVFGQALGTPCVTNIWVPDGCKESPIDRKGP
                     RERLAESLDAIFARPLDARYIRDAVEGKLFGIGSESYVVGSHEFYLSYAITRQKLLCL
                     DTGHFHPTEIVSDKISAILLSLDEVLLHVSRGVRWDSDHVVLLSDELLAIAQELVRGG
                     YLNRVHIGLDFFDASINRVAAWVIGARAMQKALLLALLEPTETLRQLEADGDTTARLA
                     LLEEIKTLPYGAVWDHYCRSRDMPAGRAWLAEVKTYERDVLSKRS"
     gene            complement(1544..1696)
                     /locus_tag="VHS97_22645"
     CDS             complement(1544..1696)
                     /locus_tag="VHS97_22645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451071.1"
                     /translation="MQRTLVFGGFGRELPRSSFYLKKSGETDRRVKVLLLVVALAGTR
                     LQLRAE"
     gene            1962..3161
                     /locus_tag="VHS97_22650"
     CDS             1962..3161
                     /locus_tag="VHS97_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451072.1"
                     /translation="MRTRRGFTLIELLVVIAIIAILIALLLPAIQSAREAARRAQCTN
                     NLKQLGLATHSYLSQQGTFPPLVQNGSFDVWGSTYSGVNGGFAGRFYDPWPLDWTASI
                     LGQLDQIPLFNQLNFAFSSGWLGPFSTLGAWDPQNTTVLATQVGVLLCQSEDKKVTMI
                     GPGTRRNYVANFGGPANFMAWSGVLVPLKDTPPLSYAGVYANANSGTTFGMEGITDGS
                     SNTAIFSETLIGSGPASPIALAATPRQSTYLWFVPFNNIWDQGPQGGVTALLFMQACK
                     SIPGITQSVGLLPPVNGNIWLAGNPGSSLMWDAYNHYMPPNSTACAASNDFNVNPGGG
                     SISTATYGGWGTFMDALPPSSNHPGGVNIAFADGSVRWVKNSVTPNTWWSLGTRNGQE
                     ALSSDAY"
     gene            3333..3578
                     /locus_tag="VHS97_22655"
     CDS             3333..3578
                     /locus_tag="VHS97_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451073.1"
                     /translation="MRQVRWVAGGVVLAFTLAGCGESAPESGTMEYKPSNSPEIEAFT
                     KRMSENAKGGKGAMKKPEAASKPGETKPASEGKAESK"
BASE COUNT          774 a         1103 c         1129 g          740 t
ORIGIN      
        1 gccggcccat gacctcgatc gtccccaggt cgggcaggtg ggcgcccgta atcaccttga
       61 ttagcgtcga tttgccagcg ccgttttcgc cgaccaaggc gtggacttca cccgcacaaa
      121 ggtcgaacga gacgctccgc aacgcctgaa ctccggcaaa cgccttgctg attcccgtca
      181 attccagcaa catcggcacg acgcgattct ccgtgtcctg acctaactgc gcttcgaaag
      241 gacgtcgcgt tcgtacgttt tgacttccgc cagccaggca cggccggcgg gcatgtcacg
      301 cgaacggcag taatgatccc agacggcgcc gtaagggagc gtctttatct cctcgaggag
      361 cgccagccgc gcggtcgtat cgccgtcagc ctcgagctgc cggagagttt cggtcggctc
      421 gagcaaggcg agcaagagag ctttttgcat ggcccgcgca ccgatgaccc aggccgccac
      481 ccggttgatg ctggcgtcga agaagtcgag cccgatatgg acccggttga gataaccgcc
      541 gcgaacgagt tcctgggcga tcgcaagaag ctcgtcggac agcagcacca cgtggtcgct
      601 gtcccagcga actccccggc tgacgtgcaa caaaacctca tcgagggaca agagaatcgc
      661 cgagatctta tccgagacga tctcggttgg gtgaaagtgt ccagtgtcga gacacaaaag
      721 cttctggcgg gtgatggcat aactcagata aaactcgtgc gagccaacaa catagctctc
      781 cgacccgatt ccaaacagct tgccctcgac ggcgtcgcgg atgtaccgcg cgtcgagcgg
      841 tcgggcgaag attgcgtcca gcgattcggc gagtcgctcg cgcggtcctt tgcggtcgat
      901 gggactttcc ttacagccgt cggggaccca gatgttggtc acgcagggtg tgcccaaagc
      961 ttgaccgaaa acggcgccga tctgtcggca cgcgatcccg tgatcaatcc aaaaccgacg
     1021 gatcgaacga tcggggtggg cgagggtgta gccgtcggcc gcgagcgggt gggcgaaata
     1081 tgtgggatta aagtccatgc cgatccgccg cgactgcgcc cagtggatcc acgtctcgaa
     1141 atgttcgggc tggagctggt cgcgctcgat cttgcggccc ttggtatcgg catagctggc
     1201 gtgtaggttc aaccggtgtg agccgggcaa gagcgacaac gtcttgtcca agtcggcccg
     1261 aagctcgtcg ggtgtacgcg ccttgcccgg ataatggccg gtgaccgcca gtccgccccc
     1321 cagctcttgg ctagagttct caaagccagc gacatcatcc ccctgccagc agtggagcga
     1381 gatcgcgatc gccgacaagc gttcgagggc aacctcgaca tcgacgcccc actccgcgta
     1441 ttgctccttc gcgccggcga aggctcgtac cacgttttcg cctggagaag ccatgtcgcc
     1501 gtctcgcacg agggcaggaa ttgtcaggat gggcattcac ggttcattct gcccgaagct
     1561 gcaaccgcgt gccagccaga gcaacgacga gaagcagaac cttgacccgg cgatcggtct
     1621 ctccgctctt tttgaggtag aatgaagagc ggggcaattc gcggccgaat ccgccgaaca
     1681 caagcgttct ctgcacgcat gtgtgtttct aacggcagat gatcccttcg acttatgtac
     1741 ggaacctcct tgcggaatgt gcctgatgca ataggatagg cgttgagggc aaatttcagt
     1801 ccgcggtctt ccttggtttt aattgaactc agtcggaacc aggagaccac ggtttgatct
     1861 ggagaaaggc tctaaagtcc tagcccgctg ggagagtaag cgggatcgaa tcgatggaag
     1921 acttcgattt caaatcctgt cgtcccgaaa ggggagatcg catgcgaact cgccgaggct
     1981 tcacgctgat cgaactgttg gttgtcattg ccatcatcgc cattctcatc gcgctattgc
     2041 tgcccgcaat ccagtcagcg cgcgaggcgg cacggcgggc ccagtgcacc aacaacctca
     2101 agcaactggg tcttgccact cacagttacc tgtcccaaca ggggactttc cccccgttgg
     2161 tccagaatgg gagtttcgat gtctggggaa gcacctatag cggagtcaac ggcggctttg
     2221 ccgggcgctt ctacgatcct tggcccttgg actggaccgc gtcgatcctg ggtcagctcg
     2281 atcagatccc gctcttcaac caattgaatt tcgcgttcag ctcaggatgg cttggcccat
     2341 tttcgactct gggggcgtgg gatccgcaga acacgacggt gctcgctacg caggttggcg
     2401 tcttactctg ccagtccgag gacaagaaag tgacgatgat cggaccggga acgcgcagga
     2461 attatgtcgc caatttcggc gggccggcca atttcatggc ctggagtggc gttctcgtcc
     2521 cgctcaagga cacgcccccg ctcagctatg ccggtgttta tgcgaatgcc aacagcggca
     2581 ccacgtttgg gatggaagga atcaccgacg gcagctcgaa cacagcgatc ttcagcgaga
     2641 ccctgatcgg ttcaggtcct gcctcgccga tcgcgctggc cgcgacgcct cgtcaaagta
     2701 cgtatttgtg gtttgtcccg ttcaacaaca tctgggacca agggcctcaa gggggcgtca
     2761 ccgcgttgct tttcatgcaa gcctgcaaga gtatcccggg aatcactcaa tcggtcggtc
     2821 tattgccgcc ggtcaacggc aacatctggc ttgccggcaa ccccgggtcg agcttgatgt
     2881 gggacgcata caaccactac atgcccccca acagcaccgc ctgcgccgcg tctaacgact
     2941 tcaacgtgaa cccagggggc ggcagcatca gcacggcaac ctacggtggc tggggtacgt
     3001 tcatggacgc tttgcctccg agcagtaatc atcccggcgg tgtcaacatt gcattcgccg
     3061 atgggtcagt acgctgggtc aagaattcgg tgactcctaa cacctggtgg tcgcttggca
     3121 ctcgcaacgg ccaggaagcc ctcagctctg atgcgtattg atcgaggttc gatcggtcga
     3181 ggacggctca gagccgtcct cgaccctcgc tccaaagatc gggtggccag tggagagaga
     3241 cagagtctga tagctggtac actcgggtcc gacacagctg ctcatcaaga gtccaaatgc
     3301 aggttttgac ttcaagacag gagtacttat cgatgcgtca agtacgctgg gtggccgggg
     3361 gagtcgtatt ggccttcaca cttgcgggct gtggggaaag cgcaccggag tcagggacca
     3421 tggaatacaa gccgtcgaat tctcccgaga tcgaggcatt cacgaagcgg atgtcagaaa
     3481 acgccaaggg cggcaagggt gccatgaaga agccagaggc tgcgagcaaa ccgggcgaga
     3541 ccaaacccgc gtctgagggt aaggcggagt cgaagtagga atagtggtga aattctcgcc
     3601 attgcgcctg cggtgatcga ctgctcgtat accgacgccc cgtgggcagt ttacttgcct
     3661 ttcagattgg cgaggaactc gatcatgtcg cgcagctcgg ctcttgagag cttgcgtgcc
     3721 aggtcattgg gcatcgccga gggacc
//
LOCUS       DATAQL010001313         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_288001, whole genome shotgun sequence.
ACCESSION   DATAQL010001313 DATAQL010000000
VERSION     DATAQL010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288001"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VHS97_22660"
     CDS             <1..325
                     /locus_tag="VHS97_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451074.1"
                     /translation="AVTMSAKSTKGHGRTPWLDEKSETPLIDDYVQRLGSFLEAMADG
                     RIEASELKTQEDRVVALMKTVEPELSDEMHQKVTHLLCELSAYNIMHTIHQLVEKQPK
                     TKFRG"
     gene            682..>2382
                     /locus_tag="VHS97_22665"
     CDS             682..>2382
                     /locus_tag="VHS97_22665"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3451075.1"
                     /translation="MAKVVICPSCQSKASIPDDAQPARIRCPKCKQTFDTGAASKSSA
                     GQAKRPASAKRYPAAVSSVHDEIQSVQPLPTLSSSSSSRRTALDAARHSSGQSPVLYV
                     VLGVGGLAIVLLFVVLGVVLTRRGGEPAANAPRAEVVQNDPPARAIEPVAAVTPIALV
                     PTSTESVPAGSSASSNIDHEEIVRRLKEATVYLKHKVGNLTLGSGTGFVIEVRGDTVV
                     LATNRHVAVFDHDEIPPSVLPKGSKVEFEAVFRSGLGPQKEQTVPATIVAADTSEDLS
                     TDLAILMVKGVKNPPTPLSAFAKSDTTEGMAYTGAGFPFGGMLGKVTENKGNPSVTIT
                     RGGIAALRRDDHGHVDLFQVDGSLQPGNSGGPIVEEKTGKLIGLAVAKKGDVDTIGFV
                     VPADELRRTLAGKVGALDLTLKTLAEKNAHLVVKAEIVDPKGAVQNVMVHVAPASAGA
                     ISPNGDGTWPPLPNTTGVELKRDPKVAMASGDVQVSLSGEGAAARKILVQTAHRDTRG
                     QLVYSKPRAIDLPEKPGRVMVSNHMIRLLKTVQRKSCSMLGPLVDPEKDCKLTKSDDD
                     M"
BASE COUNT          508 a          669 c          733 g          472 t
ORIGIN      
        1 ggcagtaacc atgagcgcga aaagtaccaa gggacatggc cgtaccccgt ggctcgacga
       61 gaaatccgaa actccgttga tcgatgacta tgtgcaacga ctcggatcgt tcctggaagc
      121 gatggccgac ggccgaatcg aggcgagcga gctgaagaca caggaagatc gggttgttgc
      181 cctcatgaag accgtcgagc ccgagctgag cgacgagatg caccaaaagg ttacgcacct
      241 gttgtgcgag ctctcggctt acaacatcat gcatacgatc catcagctcg tcgaaaagca
      301 gccgaagacc aaatttcgcg gttgatatca gcctgagcta gtgaacgcgg cggccgtcgc
      361 gttcatgcgc gcccgaaaca tcgagggacc gccccgcctg ttaatcagtc ggaggaggtc
      421 gtgcccgttt acggaacgac acacatcgct gccttttgtg ttcgaattgc tgatctcagg
      481 atcgcaacga tgaatcggtc gtggcaattc ttggattcct gaccagtgcg tgcttgaatg
      541 cgtcgcacta tttcgctgaa atgattctat tatgaactta gacaagtcca cgtgcggatc
      601 gaaggtcgat cttggctaac cggatttgct catcggtcgt gtcgtcttct ccaattcgca
      661 gagtcccgac aggagggcgt tgtggccaag gttgtcatct gcccatcgtg ccagtcgaaa
      721 gcgtcgattc cggacgatgc gcagcccgcg cgtattcgtt gccccaagtg taagcaaaca
      781 tttgacactg gcgccgcgtc gaagtcgagc gcgggccagg cgaagcgtcc ggcctcagcc
      841 aagcgttacc ccgcggcggt gtcttcggtg catgacgaaa tccagagtgt tcaacccttg
      901 cccacactca gtagtagttc gagctcgcgg cgcaccgctt tagacgcggc ccgccacagt
      961 tccggccaat cccccgttct gtacgtggtg ctgggcgtgg gtgggcttgc catcgtgctg
     1021 ctttttgtcg ttctgggggt ggttctgacg cggagaggcg gtgaaccggc ggccaacgct
     1081 cctcgagccg aggtggttca aaatgacccg ccggcccgcg caattgagcc tgtagcggcc
     1141 gtgacgccga tcgcactggt accaacttca accgaaagcg tgccggcggg ttcaagtgcg
     1201 tcgtcgaata tcgatcatga agagattgtg cgccgtctga aagaggcgac ggtgtacttg
     1261 aaacacaagg tcggaaatct gaccctggga tccggtacgg gttttgtgat cgaagtccgc
     1321 ggcgatacgg tggttttggc gaccaacagg cacgtcgccg tgttcgatca cgacgaaatc
     1381 ccgccgagcg tcttgcccaa gggaagtaag gtcgagtttg aagcggtttt ccgcagtggc
     1441 ttggggcctc agaaagagca aactgttccg gcgacgatcg tcgcggccga cacgagcgag
     1501 gatttaagca ccgatttggc gatccttatg gtcaaggggg tcaagaatcc gccgactcca
     1561 ctcagcgctt tcgccaaatc ggacacgacc gaggggatgg cctataccgg cgctggattt
     1621 cccttcggcg ggatgctggg caaggttacc gagaacaagg gaaatccatc ggtcacgatt
     1681 acacgggggg gtattgcggc tctgcggcgc gacgatcacg ggcatgtcga cttgtttcag
     1741 gtcgacggct ccctgcagcc gggcaatagc ggcggaccta ttgtcgagga gaaaaccggc
     1801 aagcttatcg gtttggctgt ggctaagaag ggggatgtcg acacgatcgg atttgtcgtc
     1861 cccgccgatg agctgcgtcg gacgctggca gggaaagtcg gcgccctcga tctgactttg
     1921 aaaacattgg cggaaaaaaa cgcacatctc gtcgtcaagg ccgaaatcgt cgatcccaag
     1981 ggagctgttc agaacgtcat ggtgcacgtg gcccccgcat ctgccggcgc catcagcccc
     2041 aatggcgacg gaacatggcc tcctctgccc aatacaacgg gtgtcgagct gaagcgcgac
     2101 cccaaggtgg cgatggcgtc gggagacgtt caggtctcat tgagcggcga aggcgccgcg
     2161 gcgcgcaaga tcctggtcca gacagcgcat cgcgatacgc gcgggcaact cgtctattcg
     2221 aagccgcgcg cgatcgacct cccggaaaag cccggtcggg tgatggtctc aaaccatatg
     2281 attcgactgc tcaagactgt tcagcgtaag agctgttcaa tgctcggtcc cctggtcgac
     2341 cccgagaagg actgcaagct caccaagtcc gacgacgaca tg
//
LOCUS       DATAQL010001314         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4408133, whole genome shotgun sequence.
ACCESSION   DATAQL010001314 DATAQL010000000
VERSION     DATAQL010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4408133"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VHS97_22670"
     CDS             <1..194
                     /locus_tag="VHS97_22670"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HEX3451076.1"
                     /translation="SRAWCMRSIASALPASDPVRATLIGSADRHATAALEHVASGDYA
                     GEHWLASFAVYLLSCTQAD"
     gene            232..1611
                     /locus_tag="VHS97_22675"
     CDS             232..1611
                     /locus_tag="VHS97_22675"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3451077.1"
                     /translation="MFRIVHAHFRGRTMRLAGLVVLLTGTIYSATSAQEPDPAKVAKP
                     SPSSAPETLTADQRAKNVEAFDMLWQTIRDKHFDPKLGGLDWQSVRDKLRPRVEQAAT
                     MKVARAIMNEAIDQLHQTHFGIIPADLYKDLESPHSGPGELGADLRIVDGRAVVTAVT
                     EGQPASVAGVKTGWTVEKIDGKPVSEILQSAEAAYAKSGMLAARKTLAVAARLRGPVG
                     SAIALDFVDQNDKPVHKDLTAVEPKGVPATFGNLPTFHVRFTSKRVDRTVSYVSLNVF
                     FDLVNVLKQYGEAIEASRDADGLIIDLRGNPGGIGAMSFAMGGWLVSERGLKLGTLVT
                     RDGSLNFTLFPRPRPFTAPVAVLVDELSMSTSEILAGGLKDLKRARVFGVRTPGAALP
                     STIEVLPNGDRFQYAFANYISANGQPLEGVGVVPDAETPWTRAALLEGRDPAVEAALC
                     WIRSSRKSR"
     gene            1664..2434
                     /locus_tag="VHS97_22680"
     CDS             1664..2434
                     /locus_tag="VHS97_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451078.1"
                     /translation="MRPKVVFSLICLAVIFGQYKASAQAKPDLPTADTVLNQYIEATG
                     GKAAYEKLKNRVSTGTIELPAANIKGTIKLTQAAPNKALLFFELGPAGETRRGSDGKT
                     VWEISTLTGERDLDGDEKDTFIRETNFYKELLWKDLYAKVECVGIEDVNGKPAYKIVL
                     TPKSGKPLTEYYDKTTHLIVKQTSTTAGPMGEITVDVFPTDFKTVDGVLIPFTTTQKV
                     LTQDIVMKLTEIKHNVELPADAFQRPGKKEEPAKTKQN"
     gene            2610..2852
                     /locus_tag="VHS97_22685"
     CDS             2610..2852
                     /locus_tag="VHS97_22685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3451079.1"
                     /translation="MVDTGSERGINGGIWPAPPQGPSMVSLYVEVDDVGAYASRATEL
                     GGKVVMPPQKLPDGDEMAIILDVEGIPVGIFKPAAV"
BASE COUNT          602 a          860 c          834 g          571 t
ORIGIN      
        1 tgagccgcgc atggtgcatg cggagcatcg ccagcgcctt gcctgccagc gacccggtac
       61 gtgcgacttt gataggttcg gccgaccgtc acgccacggc cgcactcgaa cacgtggcga
      121 gcggcgatta cgctggtgaa cactggctgg cctcgtttgc cgtttatctt ctctcgtgca
      181 cccaggctga ctgagcggct cttgtcatcg ctctggagac aattctcgat catgttccga
      241 atcgttcatg cacattttcg gggtcgcacg atgcggctcg ccggcctcgt cgtgttgctt
      301 accggcacca tttactcggc gacgagcgcc caagaaccag atcctgcgaa agtcgccaaa
      361 ccgagtccat cgtctgcgcc cgagacactg acggccgacc agcgggccaa aaacgtcgag
      421 gcgttcgaca tgctctggca gacaattcgc gacaaacatt ttgatcccaa gctggggggc
      481 cttgactggc agtccgtccg tgacaagttg cgaccaaggg tcgagcaggc tgccacgatg
      541 aaagtcgcgc gcgcgatcat gaacgaagcg atcgaccagc ttcaccagac gcatttcggg
      601 atcattccgg ccgaccttta taaagacctc gagagtcccc acagcgggcc gggtgagctt
      661 ggtgccgatc ttcgcatcgt tgacggtcgt gcggtcgtga cggcggtgac cgagggtcag
      721 cccgcgtcgg tcgcaggtgt caagaccggt tggacggtcg agaaaattga cggtaagccg
      781 gtgagtgaaa ttctccagtc ggccgaggcg gcgtacgcca aatccgggat gttggcggct
      841 agaaagactc tggcagtcgc cgcccgacta cgtggtccgg tcggatcggc cattgcatta
      901 gacttcgtcg accagaacga caagccggtc cacaaggact tgacggccgt cgagccgaag
      961 ggcgttccgg cgactttcgg caaccttccc acattccatg tgcggttcac ctccaaacga
     1021 gtcgaccgca cggtcagtta tgtgtcgctc aacgtcttct tcgacctggt caatgtgctc
     1081 aagcagtatg gcgaggccat cgaggcgagt cgcgacgccg acggcttgat catcgacctt
     1141 cgaggcaatc ctggcggcat cggcgccatg tcattcgcaa tgggaggctg gctggtcagc
     1201 gagcgaggct tgaagctcgg cacgctggtc acgcgcgacg gttccttgaa ttttaccctc
     1261 tttcctcgcc ctcgaccctt cacggcaccc gtcgccgtac tggtcgacga gctgtcgatg
     1321 tccacgtcag agatccttgc gggcgggctc aaggatctca agcgcgctcg tgttttcggc
     1381 gtcaggacgc cgggcgctgc cctcccctcg acgatcgagg ttctccccaa cggagatcga
     1441 tttcagtatg cgtttgccaa ttacatttcc gccaatggcc aaccactcga aggggtaggc
     1501 gtcgtgcccg acgccgaaac tccctggacg cgcgcggccc tgctcgaagg ccgagacccg
     1561 gcggtcgagg ccgctctttg ctggatccgt tcttccagga agtctcgttg agattgcgcg
     1621 tgcatttctc aatcgaacct ctctttttca ggagactatg tttatgcgac cgaaagtcgt
     1681 tttcagtctg atttgcttgg ccgttatttt cggccagtac aaagcgagtg cgcaggcaaa
     1741 gcctgacttg cccacagccg acacggtact caaccagtac atcgaggcca cgggagggaa
     1801 ggccgcgtac gaaaagctca agaatcgtgt atcaaccggg acgatcgaac tcccggcagc
     1861 caacatcaag ggcacgatca aactcaccca ggccgcaccc aacaaggcgc tcctcttctt
     1921 tgaacttggt cccgccggcg aaacccgccg cggttccgac ggcaagaccg tctgggagat
     1981 ctcgacattg acgggcgagc gtgatctcga cggcgacgag aaagacacgt ttattcgcga
     2041 gacgaacttt tacaaagagc tgctctggaa ggatctctac gccaaggtcg agtgcgtcgg
     2101 catcgaggat gtgaatggca aaccggcgta caagatcgtg ctcactccca agtcgggaaa
     2161 accgcttact gaatactacg acaagaccac ccacttgatc gtcaagcaaa cgtcgacaac
     2221 ggccggcccg atgggcgaaa tcacggtgga tgtcttccct accgacttca aaacggtcga
     2281 tggcgttttg atcccgttta cgactacgca gaaggtattg acgcaggaca ttgtgatgaa
     2341 gttgacggaa atcaagcaca acgtcgaact gccggccgat gcgtttcaac gtcccggcaa
     2401 aaaggaagag ccggccaaga ccaagcagaa ttgacgcgga cattcgcggc cccggtctcg
     2461 gggccgcagc atatccaagg ggcggtaaac gcgttgcacc aacccttggc atctcatacc
     2521 aggcgtgggg caattcccct gcaccagttt tcatttatac caagctcttt gactgggaca
     2581 tcaacaccaa caacgcgctc aattatcgaa tggtcgacac cggctctgag cgcggaatca
     2641 acggtggcat ctggcccgct ccgccacaag gtccgagcat ggtcagtttg tatgtcgaag
     2701 tcgacgatgt cggggcctat gcgtcgcggg cgaccgagct tggtggcaag gtcgtcatgc
     2761 cgccccaaaa gcttccggac ggcgatgaga tggccatcat actagacgtc gagggtattc
     2821 cggtcgggat cttcaagccg gcggctgtct agttcgtgct gaagccg
//
LOCUS       DATAQL010001315         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1967122, whole genome shotgun sequence.
ACCESSION   DATAQL010001315 DATAQL010000000
VERSION     DATAQL010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967122"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VHS97_22690"
     CDS             <1..442
                     /locus_tag="VHS97_22690"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3451080.1"
                     /translation="ICEVEVDAETGAVEIVGYTAVDDVGRAVNPMIVHGQTHGGIAQG
                     VGQALLEHSHYDPETGQLLAASFMDYAMPRADTFPEIKTALSEVPSPSNRLGVRSGGE
                     GGTTPALAAVINAIVDALSEFGVRHIEMPATPERVWRAIQAANS"
     gene            complement(1370..>1882)
                     /locus_tag="VHS97_22695"
     CDS             complement(1370..>1882)
                     /locus_tag="VHS97_22695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3451081.1"
                     /translation="NLAQAGATTPEMIEPLSKRVNELGWHIQINATAAKIMEIMPILD
                     RVPSPIVFDHLAHIPEPEGVNHPLFVQIRALLDKGRTWVKLSGAYADTKVGPPTYADS
                     TAVAQAYVKAAPERLVWGSDWPHPGERENKPDDAVLFDLLLQWAPDESVRHRILVENS
                     AILYDYPKSA"
BASE COUNT          364 a          585 c          611 g          322 t
ORIGIN      
        1 gatctgcgag gtagaggtcg atgccgaaac cggggcggtc gagatcgtcg gctacaccgc
       61 ggtcgacgac gtcggccgcg cggtcaaccc gatgatcgtg cacggtcaga cccatggcgg
      121 catcgcccaa ggtgtcggcc aggcattgct ggaacacagc cattacgatc ccgagaccgg
      181 ccagttgctg gcggcgagct tcatggatta cgcgatgccg cgcgccgaca cctttccaga
      241 gataaagact gcgctcagcg aagttccttc gccgtcgaat cgcctcggcg tccgctcggg
      301 cggcgaagga ggcaccacac cggcgctggc ggcggtcatc aacgcgatcg tcgacgcgct
      361 ctccgagttc ggcgttcgcc acatcgaaat gccggccacc cccgagcggg tgtggcgcgc
      421 gatccaggca gcgaacagct aaggcaaaaa attcgtcggc accgggccgt ggggcaccgc
      481 tgagcaaggt aaccgtatcg gtctccggcg aggcgcccgg gaaggcaggc tcggcttcgc
      541 gcgccgcagt ttggcctgat ccgaattccc catttatggc aagcccgcgg tagcacgttg
      601 tacaacgggg actccgcccg ccgcaccagc catcgcggcg agcatccgca cataaaggac
      661 gctttgggga tacggaccga cgccaatgcg gttgggggct ccgttttcgt cttcatccag
      721 atagttccac agaaacagcg agagacgctc ggtgccgtgg cgcggtgcgg gcagaacaaa
      781 acgttggagg cgccgaaaaa catttccggc gagatttgga gcggaatgac gagcatggcc
      841 gcggggccag gagcggtcgt ccgcagaagg tccccaatgc cagggttctt ggccatgtaa
      901 taatcgtgcg cgccccagcc gaagaccggg taacgtgcgc tcggaaagtc tcgggtcaaa
      961 gcggccaacg gaccgcttaa cgcctcgaca ggcagcccca gttccgtgtg ccagccgctg
     1021 gagatgacat agatcacctc ggttggcgag gccgacccgg gatagggctt caccggggtc
     1081 gctgaggtgc aggcaacgac cccgcataag atccagacta cggtaacgac aagaaaccaa
     1141 taccgggccg gcaccctggc ggcgaccttg agaactgacc caaatggatc tcggagtgtc
     1201 agcagcttat ccgtcattcc cgcggtaagc gggaatccag agctccgggg ctcaggcagc
     1261 tgccccctca gcccgcccgc gtttgcgcgg gggtgacggg taagctattg ataacgtgca
     1321 ccccattttg agccggcctc tcagaacgcg tctgatctga cagagccctt caagcgcttt
     1381 tcgggtagtc gtagagaatg gccgagttct cgaccaggat acggtgacgc accgactcgt
     1441 ccggcgccca ctgcaacagc agatcgaata gtacggcatc gtcgggcttg ttctcccgct
     1501 cgccgggatg cggccagtcg ctgccccaga caagccgctc gggggcagcc ttgacatagg
     1561 cttgtgctac tgccgtcgaa tcggcgtagg tcggcggccc gaccttggtg tcggcatagg
     1621 cgcccgacaa tttgacccac gtccggccct tgtcgagcaa tgcacggatc tggacgaaca
     1681 gcggatgatt gaccccctcg ggctcgggga tgtgagcgag gtggtcgaag acgatcggcg
     1741 acggtacccg gtcgaggatc ggcatgatct ccatgatctt ggcggcagtg gcgttgatct
     1801 ggatgtgcca tccgagctcg ttcacccgct tcgagagcgg ttcgatcatc tccggcgtcg
     1861 ttgctccggc ctgcgccagg tt
//
LOCUS       DATAQL010001316         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2120430, whole genome shotgun sequence.
ACCESSION   DATAQL010001316 DATAQL010000000
VERSION     DATAQL010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120430"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1501
                     /locus_tag="VHS97_22700"
     CDS             <1..>1501
                     /locus_tag="VHS97_22700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX3451082.1"
                     /translation="GVLGVDKFSTNSLASTVLVNYDPKQVTKTQVIEIVDDALASAEQ
                     PERLDDLDMHLPICTASLPLAASAQFVAAPLLVPAAALYAFTSIPTFKEARRVFIEEK
                     RIGVDALDAVVMVGCLGTMSIFPGAVCCWCLSFGRFLVKQSRDRSQKLLTSAFGKQPR
                     FVWLCRGEAEVQVPTEKLQKGDVIVVHTGEAVPVDGHVVAGMALVDQHALTGEATPAE
                     KGTGDRVFASTLLVAGEIRVTVEMSGSETASARIGQILSDTAGYKMSSQHKGERLADQ
                     AVLPTLGAGALGMAMIGPLVAVAVLNSDLGTGIRMAVPLAMLSSLTLCASKGILVKDG
                     RALEMMNQVDTVLFDKTGTLTRDQPEVGRVFTARDWSGDQILAFAAAAERKFHHPIAM
                     AILQRAEQLGLALPPTDDSQYKVGYGITVQIEGHTIKVGSKRFSESEGIELCPWAMEA
                     LSEAHRAGHTMVMVGVDDQLGGAIELRAAVRPEVQSIIKGLRDRGISHIA"
BASE COUNT          279 a          446 c          510 g          266 t
ORIGIN      
        1 ggagtgcttg gggtcgacaa gttctcgaca aattccctcg ccagtaccgt cttggtgaac
       61 tacgatccca agcaagtgac caaaacgcag gtgatcgaaa tcgtcgatga cgcgctggcc
      121 agtgccgagc aaccggagcg gctggacgat ctcgacatgc acctgcccat ctgcacggca
      181 tctctccctc tggcagcgtc ggcccagttc gtggcggccc cgctgcttgt accggcggcg
      241 gcattgtatg cgttcacgtc gatccccacg ttcaaggaag cgcggcgcgt tttcatcgag
      301 gagaagcgga tcggggtcga cgcgctcgac gcggttgtga tggtcggctg cctggggacg
      361 atgtcgatct tcccgggagc tgtgtgctgc tggtgcctca gcttcggccg attccttgtg
      421 aagcagtcgc gcgatcggtc ccagaaacta ctcacgagcg cgttcggcaa gcagccgagg
      481 tttgtctggc tctgccgtgg cgaagccgag gtccaggttc ccaccgaaaa gcttcaaaaa
      541 ggggacgtga tcgtcgtcca taccggcgag gcggtcccgg tcgacgggca cgtggtcgcg
      601 ggaatggctc tggtcgacca gcacgcgttg accggcgagg caacgccggc agaaaaggga
      661 acgggcgacc gggtttttgc ctcgacattg ctcgttgccg gcgagatcag ggtcactgtc
      721 gagatgtcgg gtagcgagac ggcgtccgcc aggatcggcc agatattgag tgacacggcg
      781 ggttacaaga tgtcgtcgca gcacaaagga gagcggcttg cggaccaggc cgtgctgccg
      841 actctgggtg ccggggctct cggcatggcg atgataggcc ccttggtggc cgtggcggtg
      901 ctcaatagcg acctcgggac cggcattcgc atggccgttc cgctcgcaat gctcagctcg
      961 ctcaccctgt gcgccagcaa ggggatcctg gtcaaggatg gccgagcgct ggaaatgatg
     1021 aaccaggttg acacggtcct cttcgacaag accggtaccc tgacacgcga tcaacctgag
     1081 gttggccgcg tgttcacggc ccgcgactgg tcgggagacc aaatcctcgc gttcgcggcg
     1141 gccgccgagc gcaagtttca ccacccgatt gccatggcca tccttcagcg ggcggagcaa
     1201 ttggggctgg ccctgccgcc aactgacgac agccagtaca aggtagggta cggcatcacg
     1261 gtccagatcg aagggcacac catcaaggta ggcagcaagc gctttagcga gtccgaaggg
     1321 atcgaactgt gcccctgggc aatggaagcc ctgagcgagg cacatcgcgc ggggcacacg
     1381 atggtcatgg tgggcgtgga cgatcagctc ggcggggcga tcgagctgcg ggcggccgtg
     1441 cgccccgagg tgcagtcgat catcaagggt ctccgcgacc gtgggatctc gcacatcgcg
     1501 a
//
LOCUS       DATAQL010001317         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5326749, whole genome shotgun sequence.
ACCESSION   DATAQL010001317 DATAQL010000000
VERSION     DATAQL010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5326749"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VHS97_22705"
     CDS             complement(<1..511)
                     /locus_tag="VHS97_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451083.1"
                     /translation="MAEDNSRRHSPAKRWFFLVLAAVSATLVLVATLVVPLWARVLIP
                     NWLIKNASFALLWSLLVVYAVVAPAILVAGGCAVFGAIVMYYRRERRALEGAMRWALL
                     GSICLIGLIVSELVSGHVIRGGQRLAVLPTDFEQQRSRRSSDFGPSRAKPKAEYQASA
                     RQGNPSDQDE"
     gene            complement(650..1531)
                     /locus_tag="VHS97_22710"
     CDS             complement(650..1531)
                     /locus_tag="VHS97_22710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3451084.1"
                     /translation="MSTTISDYIERDLAVRIGASSGSAIPLTLHALSNHYGVSPTPVR
                     AALRRLLAEGFLVRRRNGRIDVNPRSGNTGPAYPDRPSGNQLPPERAHTLEAALTTEI
                     IRKSLSGEANYLREEATAAWFGVGRTAIRQAFLRLAGRGLIVHVPRCGWRVRAFDEND
                     LAAYLEVREVLENKALALARPHLVEADLRRMLSGNAPRSGGTRLDNSLHGYLVEKSGN
                     VYLQEFFNRHGAYYASLLDFAAPETRVVAAMARQHRTILRALIAQDWTRARRELTRHI
                     RAQRPIVRELLQRIGTG"
     gene            1699..2613
                     /locus_tag="VHS97_22715"
     CDS             1699..2613
                     /locus_tag="VHS97_22715"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3451085.1"
                     /translation="MTVVWKGVFPAATTQFRPNQALDVPATMAHIDKMIEAGIHGLIM
                     LGTVGENCSLEYAEKLELLRATVQQVAKRVPVLSGVAEYTTALASRFANDAQELGVDG
                     LMVLPAMVYKSDSRETIAHFRGVARSTSLPIMIYNNPVSYSVDITPEMFVELADQANL
                     VAIKESSENVRRITDLKNLCADRYILFCGVDDLVLESLLLGAAGWVSGLVNAFPAENR
                     LLWDLATAGRWDEALEVYRWYTPLLHLDTHVKLVQYIKLAQQECGLGSELVRAPRLPL
                     VGEERERVLAIIRQGIETRPGKTLMARS"
     gene            2647..3588
                     /locus_tag="VHS97_22720"
     CDS             2647..3588
                     /locus_tag="VHS97_22720"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX3451086.1"
                     /translation="MQSRISVIDTHTGGEPTRVVISGGPELGSGTMAERLAVFRDRFD
                     TFRSAVVNEPRGSDVMVGAILLPPADPDCAAEVIFFNNVGYLGMCGHGTIGVAVALAH
                     LGRIGPGSHRLGTPVGTVTITLEGRNTVAIDNVESYRLAKNVKVTVPGLKTYLGDVAW
                     GGNWFFLVQEHGEELSVANVERLTEVTWRIRLALREQGITGAAGGEIDHIELFGPPSV
                     PCADSKSFVLCPGKAYDRSPCGTGTSAKLACLSADGKLREGEVWRQESIIGSVFEGSV
                     RIVEGHLKPRIRGTAYVNAESTLLIDPADPFAMGIRP"
     gene            3585..>4291
                     /locus_tag="VHS97_22725"
     CDS             3585..>4291
                     /locus_tag="VHS97_22725"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3451087.1"
                     /translation="MTTNSTERHVVVVGGGVIGTACAYFLMRSGWRVTVIERARIGRG
                     SSHGNCGLICPSHVLPLAEPGMVATGIKSLFQRNSPFAIKPRFDPALWSWLVHFAARC
                     NNRDMIQAGRGIQPLLLSSLSLYQDLIASESLDCEFETRGLLFAYRSQAGMESYVATN
                     ELMREAFQCVAQRYDGDAVAELEPALRTGLAGGWFYHEDAHLRPDKLMQAWRRVLEAG
                     GVTVRENCGLQSFHSRNG"
BASE COUNT          838 a         1297 c         1329 g          827 t
ORIGIN      
        1 gttcgtcttg gtcggatgga ttcccttgcc gtgctgatgc ctggtattcg gccttgggct
       61 tggctcttga cggaccgaaa tccgatgagc gccgggatct ttgctgctca aagtcagtcg
      121 gaagaacggc gagccgttgc ccgcctcgta tcacgtggcc ggagaccaat tcacttacaa
      181 tcaaaccgat caggcagatc gaccccagga gcgcccatcg catcgcgccc tcgagcgcgc
      241 gacgctcgcg ccgatagtac atcacgatgg caccaaatac cgcgcagccg ccggccacca
      301 ggatcgcggg ggcgacgacg gcatacacga ccagcaacga ccacaagagg gcaaaggatg
      361 cattcttgat gagccaattc gggattaaga cccgcgccca tagtggcacc accaacgtgg
      421 caacgagcac gagcgtcgca ctcactgcag cgagcaccag aaaaaaccag cgcttcgccg
      481 gggaatgtcg acgagagttg tcctcggcca ttcgagactc gaagtcttac aaggaattga
      541 aaagtccgac tggtgaatcc acgttcgccg cagcgtgaac gctcatgtcc caacaacgat
      601 tatgcgatac cgattacttc gattgaaaac tcgattcgca cgcgacacgc taacctgttc
      661 cgatccgctg cagtagctcg cgaacaatcg gccgttgagc gcggatatgc cgcgtgagct
      721 cgcggcgcgc gcgggtccag tcctgggcaa tcaaggcccg caaaatcgtg cggtgctgcc
      781 gcgccatcgc cgcgaccaca cgcgtttccg gcgcggcaaa gtccagtagg gacgcgtaat
      841 acgcaccatg acggttgaag aactcttgga gataaacatt tcccgacttc tcaaccagat
      901 agccatgcag agaattgtcg agacgagttc cgccgctccg cggcgcattg ccggagagca
      961 tcctgcgcag gtctgcctcc accagatggg gccgggcgag tgcgagagct ttgttctcga
     1021 gtacctcgcg gacctcgaga taggcggcca ggtcgttttc gtcaaacgcc cggactcgcc
     1081 agccgcagcg cggcacgtga acgatcaatc ctcggccggc cagtcgcaag aacgcctgtc
     1141 gaatcgccgt cctccccacg ccgaaccagg ccgcggtcgc ttcctcgcgc aagtaattcg
     1201 cctcgccgct cagactcttc ctgataatct cggtcgtcag agcggcttcc agagtgtgcg
     1261 cccgctcggg aggcaactgg tttcccgaag gcctgtccgg gtaagcggga ccagtatttc
     1321 ctgacctcgg attgacgtca atgcggccgt tgcggcggcg aaccagaaaa ccttcggcca
     1381 gcaagcgccg cagcgccgcg cgcaccggtg tcgggctcac gccatagtgg ttggacaatg
     1441 catgaagtgt cagggggatg gctgatcccg agctcgcgcc gatgcggaca gcgaggtccc
     1501 tctcgatata gtcactgatc gtcgtcgaca tactagtctt tcgtccggtc gagcaggcac
     1561 cttgcgagcc caatagcgac ctgatctctg gaagattgac accgcctcgc gatcacgatt
     1621 atgtttgaca ttgcgtacaa agtcaacttt tttggtggca ccgagccaga gcatcgtacg
     1681 gtgtcgagga gcgagcgcat gaccgttgtg tggaagggtg tatttccggc ggccacgacg
     1741 cagtttcggc cgaaccaggc tttggacgtt cccgcgacga tggcccatat cgataagatg
     1801 atcgaagccg gcattcacgg actaattatg ctgggcacgg tcggagaaaa ctgctcgctc
     1861 gagtacgccg agaagctcga gctcttgcgt gcgacggtgc agcaggtggc caagcgagtg
     1921 cccgtcttga gcggagtcgc cgagtatacg accgcgctgg catctcgatt cgccaacgac
     1981 gcccaagagc taggtgtcga tggtttgatg gtgttgcctg ccatggttta caaatccgac
     2041 tcgcgcgaga caattgctca cttccgcggt gttgcccggt cgaccagcct gccgatcatg
     2101 atttacaaca acccggtgtc gtattcggtc gacatcacgc cggagatgtt tgtcgagctg
     2161 gcggaccaag ccaacctggt ggcgatcaaa gagtcttcgg agaacgtccg gcggatcacg
     2221 gacctgaaga atctctgcgc tgatcgctac atactgtttt gcggcgtcga cgacttggtg
     2281 cttgaaagcc ttctcctggg agccgccggc tgggtctcgg gcctcgtcaa cgcgttccct
     2341 gccgagaacc ggctcttgtg ggatctcgcc actgccggcc gctgggacga agcgctcgag
     2401 gtgtatcgct ggtacacccc gctcttgcat ctcgatactc acgtcaagct cgtccagtac
     2461 atcaagctgg cgcagcagga gtgcggcctg ggctcagagc ttgtgcgcgc accgcgcctt
     2521 ccgttggttg gtgaggaacg agaacgagtc ctggcgatca tccgccaagg aattgagacg
     2581 cgccccggga agacccttat ggcgcgatca tgaatggctc gatccgccgc gaggaggtaa
     2641 ctgatcgtgc aatctcgcat ctctgtgatc gatactcaca ctggcggcga gcccacgcgg
     2701 gtcgtgatct cgggtggacc cgagcttggc tcgggcacca tggccgagcg cctggcggtc
     2761 ttccgtgacc ggttcgacac gtttcgctcg gcggtcgtga acgagccccg cggatcagat
     2821 gtgatggtcg gggcgatctt gctgccaccg gcggacccgg actgtgcggc ggaagtgatc
     2881 ttcttcaata atgtgggcta cctcggcatg tgcggccacg gcacgatcgg agtcgcggtc
     2941 gcgctggctc acctgggccg gatcgggcct ggctcgcacc ggctcgggac gcccgtcgga
     3001 acggtgacga tcaccctcga aggtcgcaac acggtggcaa tcgacaacgt cgaaagctac
     3061 cgcctggcca agaacgtgaa agtgaccgtt cccggattaa aaacctatct gggggacgtg
     3121 gcctggggcg ggaactggtt ctttctcgtg caggaacacg gcgaagagct gagcgtcgcc
     3181 aacgtggaac ggctgacgga agttacctgg cgcattcgcc tggcgttacg cgagcaaggg
     3241 atcacgggcg ccgcgggagg cgagatcgac cacatcgagt tatttggccc ccccagcgtg
     3301 ccatgcgccg atagcaagag cttcgtgctt tgtccgggca aggcgtatga tcgctcacct
     3361 tgtggcaccg gcaccagtgc caagctggcc tgtttgagcg ccgacggtaa gctccgcgag
     3421 ggagaagtct ggcggcaaga aagtatcatt ggcagcgttt tcgaaggctc ggtgcggata
     3481 gtcgagggtc acttgaagcc gcgaatccgg gggacggcgt acgtcaacgc cgaatcgacg
     3541 ttgttgatcg accctgccga ccccttcgcg atgggcatcc gaccatgaca acgaactcga
     3601 cggagcgcca tgtggtcgtg gtgggaggcg gtgtcatcgg caccgcgtgt gcctatttcc
     3661 tgatgcgatc gggctggcgc gtgacggtga tcgaacgggc ccggattggc cggggcagct
     3721 cgcacggcaa ttgcggactc atttgcccga gccacgtcct gccgctggcc gagccaggaa
     3781 tggtcgccac gggtatcaaa agcctgtttc agcgcaattc ccccttcgcg atcaagcccc
     3841 ggttcgaccc ggcattgtgg tcatggctcg tgcattttgc cgctcgttgc aacaaccgcg
     3901 atatgatcca agccggccgt ggtatccagc ccctgctcct ctcctcgctc tctctctatc
     3961 aagatcttat tgcgagtgag tcgcttgatt gcgagttcga gacacgcgga cttctgtttg
     4021 cgtaccggtc gcaagcaggg atggagtctt acgtggcgac caacgagttg atgagagagg
     4081 cattccagtg cgtggcccag cgctatgatg gcgacgccgt cgcagagctc gagcccgctc
     4141 ttcgaaccgg gcttgccggc ggctggtttt accacgaaga tgcacacctc cggcctgaca
     4201 agctgatgca ggcctggcgg cgcgtgctcg aagccggcgg agtcaccgtc cgcgaaaact
     4261 gcggtctcca gagctttcac agtcgcaacg g
//
LOCUS       DATAQL010001318         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2428451, whole genome shotgun sequence.
ACCESSION   DATAQL010001318 DATAQL010000000
VERSION     DATAQL010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428451"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VHS97_22730"
     CDS             <1..228
                     /locus_tag="VHS97_22730"
                     /inference="COORDINATES: protein motif:HMM:NF036494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX3451088.1"
                     /translation="NGTKVKNLTHLVELVRSCQDEYLTFRFAEDYSETLVFRRRAMLD
                     ATEPLMSENGIPRRGSDDVLAIWSGKVAKSD"
     gene            435..>1941
                     /locus_tag="VHS97_22735"
     CDS             435..>1941
                     /locus_tag="VHS97_22735"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX3451089.1"
                     /translation="MRRSKLGISFVLVGVVLGGQAWAQTSKTPDIVAVGKKATALVEV
                     TSPGADGGGSGTAFCIDKLGLFITNAHVVEEAEGGRADIRIVLDLGQKRQIRRAKILR
                     SDELLDLSLLEIDPVPGLAALELGHDDRLVETMPVTTFGYPFGRVMTFRRDGYPDITV
                     LESKITSLRRDEGQLRKIQFDNQLNPGNSGGPVLASDGKVVGVAQATVKGSALNFAIP
                     VGLLQGFLKAPAILFNPPPLAYKDRAKPVTWTIKVQAPTASAPPAAGLSVSVRIATDV
                     TTPRSFSATPAGNGTYRVTLTPVPPESETPVELRVLINGQWLETAVTDRVLRVGKASL
                     RLSDLQQLMENPPRVITRKREIVVGPISNLGKAKAPAGRQGKQVTVDLSQASVIHVGP
                     SAAAKPLRAIEAIVELKQGNEVLATKAKRMEFPDAPVLVVDRARGIGMVVQPAAPQVI
                     RGQQGPSDDGLLKIGGVLDVDGLPCGAGKAIRPPSIAMGEARLGDAPPSAAG"
BASE COUNT          382 a          606 c          624 g          329 t
ORIGIN      
        1 aacgggacga aggtcaagaa tctcactcac ttggtcgagc tggtccggtc gtgccaggac
       61 gaatacctca cgttccggtt cgccgaagat tactccgaaa ccctggtctt ccgtcgcagg
      121 gcgatgctcg atgcgaccga gccgctcatg agcgagaacg gcattccccg ccgcggcagc
      181 gacgacgtgc tggcgatctg gagtggtaag gtcgcgaaat ccgactgagc gggaacggaa
      241 tgccgaggcc cggtcgagtc agatcaaaag acgagaggcc agaccgcggc ggagggactg
      301 ggcgagctct gatcggtcca gctctatcaa caacatacat aagcggctcc cgatattcgc
      361 gtcatgaggt tctcgtggac caccggactc cggaattcga gcacgttcgt ttctcaaggc
      421 ggaagggaag tttcatgcgt cgatcgaaac tcggtatttc ctttgttctg gtcggggtgg
      481 tcctcggggg acaggcgtgg gctcagacct ccaagactcc cgacatcgtg gcggtgggca
      541 agaaggccac ggcgctggtc gaggtcacga gtccaggggc ggatggcggc ggatcgggaa
      601 cggcgttctg catcgataag ttaggcctct tcatcaccaa cgcccatgtg gtcgaggagg
      661 cggaaggggg tcgggccgac atccggatcg tgctcgacct cggacagaaa cggcagatcc
      721 ggcgtgccaa gatcttacgc tccgacgagc tacttgacct gtcgctgctg gagatcgatc
      781 cagttcccgg actggccgca cttgagctgg gccacgatga tcggttggtt gaaacgatgc
      841 cggtgacgac cttcggctac cccttcggcc gcgtcatgac gttccgccgc gacggctacc
      901 ccgatatcac cgtgctggaa agcaagatca cgtcgctgcg gagagacgag ggccagctac
      961 ggaagatcca gttcgacaac cagctcaatc cgggaaactc cggcggcccg gtgctcgcaa
     1021 gcgacggcaa ggttgttggt gtggcccagg cgacagtcaa gggctcggcc ctcaacttcg
     1081 cgatcccggt gggactgctc cagggcttcc tgaaagcccc ggcgatcctc ttcaacccac
     1141 ctcctcttgc ctacaaagac cgcgccaagc cggtgacctg gacgatcaag gtccaggcgc
     1201 ccacggcgtc cgcgccgcct gccgccggcc tctcagtctc cgtgagaatc gccactgacg
     1261 tgactacgcc tcgaagcttc tcggccacgc cggcgggcaa cgggacctac cgggtgacgc
     1321 tcacgcccgt gccaccagag tcggaaacgc ctgtcgaact gcgtgtcctg atcaacggcc
     1381 aatggctcga gacggccgtt acggatcgcg ttcttcgggt gggcaaagcc tcactccggt
     1441 taagcgatct gcagcaactg atggagaacc cgcctcgcgt catcacacgt aaacgggaaa
     1501 tcgtcgtcgg gccgatctcg aacctaggca aggcgaaggc acctgcggga cgccagggga
     1561 aacaggtcac ggtcgacctc tctcaggcta gcgtgatcca cgttggtccc tctgcggctg
     1621 ccaaaccgct gcgagcgatc gaggcgatcg tcgaactcaa gcaaggcaac gaagtccttg
     1681 cgaccaaagc aaaacgcatg gagtttcccg atgcgccggt cctggtggtc gatcgagccc
     1741 gaggaatcgg gatggtggtt cagccggccg ccccgcaggt gattcgaggc caacagggcc
     1801 cgagcgacga tggcctgctc aagatcggcg gcgtcctgga cgtcgacggg ctgccttgcg
     1861 gcgccggcaa ggcgattcgg ccgccatcga tcgccatggg cgaggctcgc ctcggcgatg
     1921 ccccaccgtc cgcggcggga a
//
LOCUS       DATAQL010001319         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2732529, whole genome shotgun sequence.
ACCESSION   DATAQL010001319 DATAQL010000000
VERSION     DATAQL010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732529"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..2091
                     /locus_tag="VHS97_22740"
     CDS             217..2091
                     /locus_tag="VHS97_22740"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX3451090.1"
                     /translation="MRLLSGVITLTAISASAQTPAVPSRSTDLEALTRDPSQWVMPSH
                     DYASTRFSTLDQINTRNAGELQVAWTFSVGADRGQEAAPLVVNNTMYVISPYAGPHPN
                     EVFALDATTGDLKWSYAPKPEIAAQGVACCDVVARGVAYDQGKIFLNTLDNYSVAVDA
                     NTGQELWHTKLGDLNQGQTITMAPLVVKGKVLVGNSGGELGVRGWVTALDENTGQIVW
                     RAYSTGPDSDVLIGPEFKPFYDWLKGKDLGAKTWPANAWKTGGGTMWGWISYDPELNL
                     VYYGTGNPGPWNSNQRPGDNLWTTSIFARDPDTGAAHWAIPLNPHDLFDHDEVNENVL
                     VDLKIDGRLRKVLIHPGRNGYMYVIERTTGEILSADPFDDLTATKGVDIKTGRPIENA
                     EKAPALGKTVEDVCPAAPGAKDWQPTAWSPRTHWLYVPHQHLCMNYQTSDVGYIAGTP
                     FVGATVDMYSGPGGYRGEFMAWDPIERKRVWSIHENFPVWTGTLVTAGDVAFYGTLDR
                     WFKAVDARNGALLWQFHAGSGFVGQPITYRGADGAQYLAILAGAGGWPAAVAAAKIDP
                     RVRNAALGFVGATQDLPKYTRGGGELLVFRLAKGQSTPDDNPAHANGAQLVPPDARDR
                     "
     gene            2201..>2630
                     /locus_tag="VHS97_22745"
     CDS             2201..>2630
                     /locus_tag="VHS97_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451091.1"
                     /translation="MCADPNNLPFSNAAGGGFENKLAELVAGELVEPVTYTWWAQRRG
                     FIRNTLNAGLCDVVMGVPVDYGLVETTRPYYRSSYVFVSRTDRGLHIRSIKDPRLKTL
                     RIGVHLIGSDGTNTPPSEALGLQNIVDNVVGYMIYGDYREP"
BASE COUNT          508 a          856 c          808 g          458 t
ORIGIN      
        1 agccaggacc aagccgtcgc cgatgccggc gccgcggctt ttaaaacctg aaacatccat
       61 tcccaaggcc gatgggcacg cggtctgaaa cgttggttga ttgtcactgc ccatccccgc
      121 gggagtcctg atgcagctcg attgaggacg acaatgtatc aacgcgcccg ggcgagagcg
      181 gcaaccgcct cctttcagca tcatatccgc agcggcatgc gccttctaag cggggtaata
      241 accctcactg cgatatctgc atccgcccag actcccgctg ttcccagccg ttcaacggat
      301 ctcgaagctc tgacccgcga cccgagccaa tgggtcatgc cttcccatga ttacgcgagc
      361 acgcgtttca gcacgctcga tcagatcaac acgcggaatg ccggggaact tcaggtagcc
      421 tggacattct cggtcggtgc cgaccgcggc caggaagcgg ctcccctggt cgtcaacaac
      481 acaatgtacg tcataagccc ctacgccggt ccgcatccca acgaggtgtt cgccctcgac
      541 gcgaccaccg gtgacctgaa atggtcgtac gccccgaagc ctgagatcgc cgcgcaggga
      601 gtggcatgct gcgacgtggt cgcgcgcggc gtcgcctacg accaaggaaa gatttttctc
      661 aataccctcg ataactacag cgtcgccgtc gacgccaaca ccggccagga actctggcat
      721 acgaagctcg gcgacctcaa tcagggccag acgatcacca tggcgccttt ggtcgtcaag
      781 ggaaaggttc tggtcggcaa cagcggtggc gagttgggtg tgcgcggttg ggtgacggcg
      841 ctcgacgaaa acaccgggca gatcgtctgg cgcgcctatt cgacggggcc cgactccgat
      901 gttctgatcg ggcccgagtt caagccgttc tacgactggc tgaagggcaa ggatctcggc
      961 gcgaagacct ggccagccaa tgcctggaag accggcggcg gcaccatgtg gggctggatc
     1021 tcctacgatc ccgagctcaa cctcgtctac tacggaaccg gcaatcccgg accctggaac
     1081 tcgaaccaac ggcccggcga caatctttgg acgacctcga tcttcgcccg tgaccccgac
     1141 acgggagcag cacactgggc gattccgctc aatccccatg accttttcga tcacgacgag
     1201 gtcaacgaga acgtgctggt cgacctcaag atcgacggcc gcctgcgcaa ggttctgatc
     1261 catcccgggc gcaacggcta catgtacgtc atcgagcgga cgaccggcga aatcctgtct
     1321 gccgatccgt tcgacgacct cacggcgacg aagggggtcg acatcaagac cgggcggccg
     1381 atcgagaacg cggagaaggc tcccgctctc ggcaagaccg tcgaagacgt gtgccccgcg
     1441 gcgcccgggg cgaaagactg gcaacccacc gcgtggtcgc cgcgaaccca ttggctctac
     1501 gttccgcacc agcatctctg catgaattac cagacatctg acgttggcta tatcgccggt
     1561 acgccgttcg tcggtgcgac ggtcgacatg tactctgggc ccggcggtta tcgcggcgaa
     1621 ttcatggcct gggatcccat cgagcgaaaa agagtctggt ctatacatga gaacttccca
     1681 gtatggactg gcaccctcgt caccgccggg gacgtggcct tctatggtac gctcgaccgc
     1741 tggttcaaag ccgtcgacgc aaggaacggc gctctgctgt ggcaattcca cgcgggatcg
     1801 ggcttcgtcg gtcagcccat cacctacagg ggcgcagacg gggcgcagta tcttgccata
     1861 ctggccggcg ccggcggatg gcccgcggcg gtggctgcgg ccaagattga tccgcgcgtc
     1921 cgcaacgcgg cgctcggctt cgtcggcgcg acgcaggacc tccccaagta cacgagaggc
     1981 ggtggcgagc tgctcgtctt caggctcgcc aagggacaga gtacgccgga tgacaatccc
     2041 gcgcacgcca acggcgcaca gctagtgccg cccgatgcgc gcgatcggta gcggcgatcg
     2101 gaacgggcgc tcgcgccgcc tcgcgttggc ggcctttgag ctcgcgatcg gcttggccgc
     2161 gccttgtctc ctcctcgcgg cggagagcga tcatctccgg gtgtgcgcag atccaaacaa
     2221 cttgcctttc tcgaacgcgg cgggtggcgg attcgagaac aagctggccg agctcgtggc
     2281 aggggagttg gtcgagcctg tgacttacac ctggtgggca cagcggcgcg ggttcattcg
     2341 caatacgctc aatgccggat tgtgcgatgt cgtgatgggc gtgccggtcg actatggcct
     2401 cgtcgagacc acgcgaccgt attaccgctc gagctatgtt ttcgtctcgc gcaccgaccg
     2461 cggtcttcac atccggtcga tcaaggaccc gcggctgaaa acgctcagga tcggcgtcca
     2521 cctcatcggc agcgacggca cgaacacgcc gccgagcgag gcgctcggac tgcagaacat
     2581 cgtcgacaat gtggtcggct acatgatcta cggcgactat cgcgagcccg
//
LOCUS       DATAQL010001320         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2272514, whole genome shotgun sequence.
ACCESSION   DATAQL010001320 DATAQL010000000
VERSION     DATAQL010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272514"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..693)
                     /locus_tag="VHS97_22750"
     CDS             complement(160..693)
                     /locus_tag="VHS97_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX3451092.1"
                     /translation="MIDAKAIERFMAAPWLAEVDPDIKRAISGVLVEARASIGATLLE
                     QGQPNDHLTFLIAGTAEIVRTFGSGRREIITDLAAPAVFGTTSFFQPKPPTVTVKATS
                     DVWILSLYHPAHEALRAANPRAAEALALAVVRALSERFDLIDKLFTEFTMQHSDEPAR
                     KSEWATFRARLFEERGI"
     gene            complement(1933..2310)
                     /locus_tag="VHS97_22755"
     CDS             complement(1933..2310)
                     /locus_tag="VHS97_22755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451093.1"
                     /translation="MADRSAAPVAQPKSRQVRRSSALVQRAPAASQALKELRAAAAVF
                     PPLPSVSRLQGPAASTYPAGLDLLPVDPEHWGERVVASGAQAAVAAESITGAAYLAQA
                     RRDPASVASRAQIPNSGRSPVRV"
     gene            complement(2784..>3238)
                     /locus_tag="VHS97_22760"
     CDS             complement(2784..>3238)
                     /locus_tag="VHS97_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3451094.1"
                     /translation="RMPRGQFGGIRHQAIVQTVDLLPTIVSALGLPPIVREEAPLHGQ
                     DLLPLIRGEQTKIRDYACLGMDVAEFAIRTHLWHLVVPIEYDPDEPRTAELFRKPEDR
                     WDQNNVIEQNVEVAEHLELALRRFVEALKRDALEELPFLRDVARLGPI"
BASE COUNT          645 a         1048 c          959 g          586 t
ORIGIN      
        1 caagagtctc actccagaca ttgtgcggtc caattgcgcg cgcgtcaacc tccgacactc
       61 gatcgattgg taatcgttga ttatttgccc ttggaaaatg ggaacccgtc atgatcggtt
      121 cagtctatcg gccgggaaag cgaagccgca gcaggattct caaatcccgc gttcctcaaa
      181 taaacgagca cgaaatgtcg cccactcgct ttttctggcc ggctcgtcgg aatgctgcat
      241 cgtgaattca gtaaacagtt tatcgatgag gtcaaaccgt tcggacagtg cccgaacgac
      301 cgcgagcgcg agggcttcgg cagcgcgagg gttggccgcg cgcagagcct cgtgagcagg
      361 atgatagagc gacaggatcc agacgtccga ggtggccttg accgtgaccg ttggcggctt
      421 gggttgaaag aacgatgtcg taccgaacac cgccggcgcc gccaggtcgg tgataatctc
      481 tcggcggcct gaaccgaatg tgcgaacgat ttccgccgtc cccgcgatca agaacgtcaa
      541 atgatcgttc ggttgacctt gctcgagcag ggtagcaccg atcgacgccc gggcctcgac
      601 cagcacccca ctgatcgccc tcttgatgtc agggtcgact tccgcgagcc acggtgcagc
      661 cataaaccgt tctatagctt tggcatcgat catctgcgaa cctcgaaata agcccaacaa
      721 aacctcgtat ccgatgccgg cacgcaacac tgtgtctcac cggaaatttg gtcccttgtc
      781 ggagcggagg agaggataaa gctcccatac tagccgattg tattgttcac aatgtgcggg
      841 atgcggcaat tagaaagtct aattggaacg agcgaatcat gacgacctta aaacgggccg
      901 acagccgaat caatcggatg gtggcaggcg ccgtgccgat gaagacgtac acggaaccaa
      961 atcggcaaag attcggctcc agggactgga gcggaaggtc aattgaggtc gaggcgggag
     1021 gaaacgccat gagacgaacg ccggcggtcg cgctggtcgg gttgttgctg ggactttccg
     1081 gctgtgccgg tgttccgcaa cggttgggct ggtcatcgcc cgcgacggat cgttccgacc
     1141 cggctgatac ctcggcgccc ggtcgattga gctggtggcg ctggccacgt acggaaaact
     1201 cgacgccgga ccaagctacc aaaatcgccg aaacccgcga aaccacccaa ccggaattga
     1261 cgaccaaact gccgacggac gtgtggccag agtctcggtc cgagtggctc gcgcggcgtt
     1321 tcccgctatt gaatcgagcc ttgaacggac cgacggtctc agctgcggtt gatagcgacg
     1381 cacgcgtccg cgaactcaac ggccgtttgt tagctccctg gtcgactgcg acgggcagga
     1441 ccagaggtgg tcgggacgac aacgacgtac ggccggtcga cgcgacggcc actaacgaag
     1501 acgcctccgg ggatcgccag accgttcccg ggcagcgctc agagcggatg ccaccgctgt
     1561 tggcgacccc cttggtcgtc aaggtgcctc cgcactcctt acctgagagc tcgggcgacg
     1621 ttgcgcttga cattaaccac gcggagcctc gccaagccag ccaagtccaa tctcaatctg
     1681 atgaggcgga aatgccgtcg gcagaaactg gaccggcgtc gtcttcgacc gtcgattcgg
     1741 cgtcggtttc gtcgacgtcg tccggcgtcc gcccaaccag cgaattgccc gccagcagtc
     1801 agcccaacgc gtcgacgggt tccgcacaac ccgagccagc ttcgtcttcg cccgtcgact
     1861 cggcgtcggt cgtagctatg tctgccatcg accgttcctc cagcgagccg cccgccaata
     1921 acgcggcgga tgctacactc gaaccggtga ccgccccgag ttcggaatct gggctcgaga
     1981 cgccaccgac gcaggatccc gccgcgcctg cgccaaatac gcagcccccg ttatcgattc
     2041 cgccgccacc gccgcctgtg cgccagacgc caccacccgc tccccccaat gttccggatc
     2101 gaccggcagc aaatccagcc cagccggata cgtcgacgcc gccggcccct gaagtcgcga
     2161 aaccgaaggg agcggaggaa acaccgccgc agccgcccgc agctccttca acgcctgaga
     2221 cgccgcaggt gcccgttgca ccagcgccga cgaccgtcgc acctgccgcg acttcggctg
     2281 cgccaccggc gccgcgcttc ggtcagccat ttgtttccgg tacttaccgg ggactttacg
     2341 cgtcgccgcc tccgatggct cccccgcggc cccggcacaa attcctcggt ctatttttcg
     2401 tggaagatac gccacagccg ctcgcgacac ctcagttgcc accggcgatg ttccccgcaa
     2461 gttatcgcgt ccagacgcca ccgccgccac cagtttttgc ggcgtcgcag gcggttgccg
     2521 ctacgccgcc cccggttgct tgcgatgcct cgccaaagcc ctgtgtgctg tcggtgctat
     2581 ttcagaagat caagagctgc ggcaaacgat cgggttgctc gggctgccat cacggcggat
     2641 cagcaccatg ctgccagggt tgcacctgtt acaccgggac caacaagccg gcacaggtat
     2701 ctcctcaggg gagcctgaca tccccacagg gcaaatcggc atactgtcag ggccatccga
     2761 tgtcgcaggc ggcacaactc ggttcaaatg ggaccaagcc tggcaacgtc gcggaggaag
     2821 ggaagctctt cgagcgcgtc tcgtttgaga gcttcgacga aacgacgcag cgctagctcg
     2881 aggtgctctg ccacctcgac gttttgttcg atgacgttgt tctgatccca gcggtcctca
     2941 ggcttcctga agagctcggc ggtgcgcggc tcatcagggt cgtattcgat cgggacaact
     3001 aggtgccaga ggtgagttcg gatcgcaaac tcggcgacgt ccatccccag gcatgcgtag
     3061 tcgcgaattt tggtctgctc gccccgtatc aagggcagca agtcttggcc gtgcaacggc
     3121 gcttcctcgc gcactatcgg cggtagaccc agggccgaca cgatcgtcgg taacaggtcg
     3181 accgtctgaa cgatggcttg atgccggatg ccgccaaact gtccgcgcgg catccgga
//
LOCUS       DATAQL010001321         4563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1967714, whole genome shotgun sequence.
ACCESSION   DATAQL010001321 DATAQL010000000
VERSION     DATAQL010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967714"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1959)
                     /locus_tag="VHS97_22765"
     CDS             complement(154..1959)
                     /locus_tag="VHS97_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX3451095.1"
                     /translation="MVDRNLLREFDVSDDELTALVTAEDGTNTLDHFLGGGQSFEIGR
                     IVSGKVVEVVGDQVVVDVGYKSEGLVPLGEWEDEPSPVAGDEVEVLLEGMEDETGEIV
                     LSRKKAHRMRAWEMVISRYHEGDVVKGRVTRKIKGGLLVDIGVNVFLPASQVDIRRPS
                     DIADYIDQEIECMILKIDESRRNIVVSRRKLIEITRETQKKQLLEEISVGQVRKGTVK
                     NIADFGAFVDLGGIDGLLHITDMSWGRINHPGDMLKIDDQVEVMVLHVDKDREKIALG
                     LKQKSASPWENVEKKYPVATRVTGEVVNVMSYGAFVKLEEGIEGLVHISEMSWTKRIN
                     HPSELVNIGDKIEVVVLGINRDKQEISLGMKQTQVNPWDQVAGKYPTGTMVDGTVRNL
                     TNYGAFIEIEEGIDGLLHISDMSWTRKIGHPNEILEKGQKISCQVLNVDQDRKRIALG
                     LKQLKDDPWETDIPGRYEPNDVVKGKVTKLTNFGVFVELEPGLEGLLHISELADHKVD
                     SPEEVVKVGDEIEVKILRVDRGERKIGLSRKKAHWTKPESEREEGAQPGESAAEPAKE
                     SKELKGGLGEGGPLFSIGGTTASTDEQTAEESAAS"
     gene            complement(2257..3129)
                     /locus_tag="VHS97_22770"
     CDS             complement(2257..3129)
                     /locus_tag="VHS97_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451096.1"
                     /translation="MTLTRWALVVLAALTIGQAPPNGNVYILVDGSRCPPQGDDPKPA
                     FVALDLQKNRINPPGPADIDHDATLAALLAPGTDIDRFDATKGATIEGIVVRVKAGSK
                     ETCNCHATNPIDQDTHIELALSGSATRIQRVVVEVTPRIRKQMKDAGQDWSTKAFQGQ
                     DSADGIVGKWLRITGWLFFDDIHLRIAENTNPGGRNNVRATCWEIHPVTALEVLDSPP
                     AGAHELHPDLLAQLQKAQVKTIATSPALREEIARRNDSLLKKYGDEAVREAEEEAKAP
                     PMPEEIRKARSLRN"
     gene            complement(3230..>4563)
                     /locus_tag="VHS97_22775"
     CDS             complement(3230..>4563)
                     /locus_tag="VHS97_22775"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX3451097.1"
                     /translation="KWICAVLAALGVGTNAALGVGTNIDAHAQDTTEFRVGAADKDIT
                     PPQGIPLWGYGARHDAPAIGALDPLMAKAIVIAAGARKVALVGIDLGRGPTEAMMKTI
                     RREIAAKAGIEHVLITGSHTHHGPVIELIDEPGLGQGKFDTAVAYSQKLPVLLVEAIL
                     AADRDSKPARIGVAIESTTLNRNRHTKRQPKATDPMLAVVRFDDLDGKPIAILVNFAA
                     HPVMTEEKLLKYSADYPGFLKKKVEAELTAKCIFMQGASGDMSPNPNPGPWEPKSFGE
                     TVADHVIALAQSATTEIPKHPSVSGMVDTFRFQTRVNLKDPRVAAVFERAYFPEITRN
                     YVKLYGDGLTAELNTVVVNGELALVGGSGEFFCNHANRLKARSYVKNTLFFGYCNGHG
                     MYFPTIEAASEGGYGADAGVSLVELGGGERMMDKALINIYILLGKFASERR"
BASE COUNT          855 a         1364 c         1317 g         1027 t
ORIGIN      
        1 tgcagatttt ggggaatgag cggcttcagg cagacgcatc ggtatccgag cgttcagtcg
       61 agacccgcgc aaaaaagaaa ccgtcgccag gcggagagcc cggagacggt ttggggaaga
      121 atcgattgtg gatatcttac cagcgagtaa agatcaggac gcagcggact cctcggccgt
      181 ctgctcgtcg gtggaagccg tagtccctcc gatactgaag aggggtcctc cctcaccgag
      241 accgcccttc agttctttgg actccttggc cggttcggcc gccgattccc ctggttgagc
      301 accttcctcg cgctcggatt cgggcttggt ccagtgagcc ttcttgcgcg agaggccgat
      361 tttgcgttcg ccgcgatcga ccctgaggat cttgacctcg atttcgtcgc ccaccttgac
      421 gacttcctcg gggctatcca ccttgtggtc ggcgagttcg gagatgtgta agagtccttc
      481 cagccccggc tcgagctcga cgaaaacgcc aaaattcgtg agcttcgtga ccttaccctt
      541 gacgacgtca ttgggttcgt agcggccggg gatgtcggtc tcccagggat cgtccttgag
      601 ctgtttcaag cccagggcga tccgtttgcg gtcttgatcg acgttcaata cctggcagct
      661 gatcttctgg cctttttcga ggatctcgtt ggggtggccg atcttgcgcg tccagctcat
      721 atcggagata tgaaggaggc cgtcgattcc ctcttcaatc tcgatgaagg ccccgtagtt
      781 ggtaaggttg cgaacagtgc cgtcgaccat cgtgccggtc gggtatttac cggcaacctg
      841 atcccagggg ttgacctggg tctgcttcat gccgagagaa atctcttgct tgtcgcgatt
      901 gatacccagg acgacgacct cgatcttgtc tccgatattg accagctcgc tggggtggtt
      961 gatccgcttg gtccagctca tttcggagat gtggaccagt ccctcgattc cttcctcgag
     1021 tttgacaaac gcaccatagc tcatgacgtt gacgacttcg ccggttaccc gggtcgcgac
     1081 cgggtacttc ttctcgacat tttcccacgg gctggcggac ttctgcttga ggcccaaggc
     1141 gatcttttcg cgatccttgt cgacgtgcag gaccatgacc tcgacttgat cgtcgatctt
     1201 gagcatgtcg ccggggtgat tgatccggcc ccagctcata tcggtgatat ggagcagccc
     1261 gtcgatgccg cccaagtcga cgaaggcgcc gaagtcggcg atgtttttga ccgtcccctt
     1321 gcggacctgc ccgacactga tctcctcgag tagctgtttc ttttgggtct cgcgcgtgat
     1381 ctcgatcagt ttgcggcggc tgacgacgat attgcgccgg ctctcgtcga tcttcaagat
     1441 catgcattcg atttcttgat cgatatagtc ggcaatatcg gatggacggc ggatgtcgac
     1501 ctgactggcc ggcagaaaga cattcactcc gatgtcaacg agcagcccgc ctttgatctt
     1561 gcgcgtgacc cggcccttga cgacgtcacc ctcgtgataa cgggagataa ccatctccca
     1621 tgcacgcatc cgatgcgcct tcttgcgcga caagacgatc tcgccggtct cgtcttccat
     1681 gccctccagc aagacctcga cctcgtcgcc ggcgaccggc gacggctcgt cctcccattc
     1741 gccgagcggg acaagacctt cggatttgta gccgacatcc acgacgactt ggtcgcccac
     1801 aacctcgacg accttgcccg aaacgatgcg accgatctcg aagctttggc ccccgcccag
     1861 aaagtggtcg agcgtgttgg tgccgtcctc ggcggtcacc aaggccgtga gctcatcatc
     1921 actaacatca aattcacgaa gaaggttacg atctaccatg ttggagtttc gtttccctat
     1981 ccgcacccca ttttttgggg aggtgcggac cgacgcgtga gccgccgatc agactgtgcg
     2041 cgcccgcccc tttgcccatg cagcaatgat tggtggaaac gaacagagat ctcgacggca
     2101 taagactttt cggttgaaaa aaaacaccac ccctcaacgc aaggagcggt ccgacgcaaa
     2161 ccgctattat atagctgatc tgcctttaca caacagacgg tttgtgctgg gagccagcgg
     2221 cggtcgaaga aatgggaaat cgcgacgata ccatcatcaa ttgcgcagcg atcgggcttt
     2281 cctgatttcc tcgggcatcg gcggcgcctt ggcctcctct tccgcctcac gcaccgcttc
     2341 gtcgccgtat tttttcaaca gggagtcgtt acgacgcgcg atctcctcgc ggagagccgg
     2401 cgaagtcgcg atcgtcttga cctgggcttt ctgaagctga gccagcagat cggggtgaag
     2461 ttcgtgggca ccagcgggcg ggctatccaa aacctcgagc gcggtaaccg ggtgaatttc
     2521 ccagcatgtg gcacggacgt tgttccggcc gccgggattg gtgttctcgg cgatcctgag
     2581 gtggatatcg tcaaagaaaa gccaaccagt gattcgcaac cactttccta caatgccatc
     2641 ggcactatcc tgcccctgaa acgccttcgt tgaccagtct tgtccggcgt ctttcatctg
     2701 cttgcgaatt cgtggggtca cttcaaccac aacgcgctgg atccgcgtcg ccgaaccaga
     2761 cagggccagt tcgatgtggg tatcctggtc gatcgggtta gtcgcgtggc agttgcaagt
     2821 ctccttcgaa cctgctttca cgcgcacgac gattccctca atggtcgcgc cttttgtcgc
     2881 gtcgaaccgg tcgatatcgg tgccaggcgc aaggagtgct gcgagcgtcg catcatggtc
     2941 aatgtccgcc ggaccgggag gatttatacg gtttttctgg aggtccaggg cgacaaaagc
     3001 tggcttcgga tcgtccccct gtggcgggca ccgtgagcca tccaccagaa tgtatacgtt
     3061 gccattcggc ggcgcttgcc cgatcgtcag tgccgccagc accacgagag cccatcgcgt
     3121 cagtgtcatc ttatatctcc acgctctggc cttgagtgat cgcccgaatc cggaacctgc
     3181 tttgcccatc atatgcgctc ttggcgggtg acttagctgg caattgcatt caccgtcttt
     3241 cgctggcaaa cttgcccaat aatatgtaaa tattgatcag tgccttatcc atcatccgtt
     3301 caccgccgcc aagctcgaca agagatactc cggcatcggc gccgtagccc ccttccgagg
     3361 cggcctcaat ggtggggaag tacatcccat ggccgttgca gtagccaaag aagagggtgt
     3421 tcttgacgta cgaccgcgcc ttgagccggt tggcatgatt gcagaagaat tcgcccgagc
     3481 cgcccaccaa ggcgagctcg ccgttgacga caacggtatt taactcagcg gtgagtccgt
     3541 cgccgtatag cttcacataa ttacgggtga tttccgggaa gtaggctcgc tcgaatacgg
     3601 ccgccacacg gggatccttg aggttgacac gggtctggaa gcggaatgtg tcgaccatgc
     3661 ccgacaccga gggatgtttc gggatctcag tcgtggccga ctgcgccagt gcgatcacgt
     3721 ggtcggcaac cgtctcgccg aagctcttcg gctcccacgg gcctggattc ggattggggc
     3781 tcatatcgcc ggaagcgcct tgcatgaaga tacacttcgc agtcagttcc gcttcgacct
     3841 ttttcttgag gaagccgggg taatcggcgg agtatttgag gagcttttcc tcggtcatga
     3901 cgggatgcgc cgcgaaattg accaggatcg cgattggctt gccgtccagg tcatcgaacc
     3961 ggacaaccgc cagcatggga tcggtggcct tcggctggcg tttggtgtga cgattgcggt
     4021 tcaaggtggt tgactcgatt gccacgccga tcctggccgg cttgctgtcg cgatcggccg
     4081 cgaggatcgc ctcgacgagc aagaccggga gcttttggga ataggcgacg gccgtgtcga
     4141 actttccctg gccgaggccg ggttcgtcga tcagctcgat gaccgggccg tggtgggtat
     4201 gacttccggt aatcaagacg tgctcgatac cggccttggc ggcaatctcg cggcgaatgg
     4261 tcttcatcat cgcttcggtc ggtccgcggc ccaggtcgat gccaacaagc gcgaccttgc
     4321 gagcgcctgc cgcgatcacg atcgccttgg ccatcaaggg atcgagcgcg ccgatcgccg
     4381 gggcgtcatg gcgagcacca tacccccaca acggaattcc ctgcgggggc gtaatgtcct
     4441 tatccgccgc gccgacacga aattccgtcg tgtcctgggc atgagcatca atattagtgc
     4501 ccacgccgag cgcggcatta gtgcccacgc cgagcgcggc caaaaccgcg cagatccact
     4561 tta
//
LOCUS       DATAQL010001322         4436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1816412, whole genome shotgun sequence.
ACCESSION   DATAQL010001322 DATAQL010000000
VERSION     DATAQL010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816412"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            626..1150
                     /locus_tag="VHS97_22780"
     CDS             626..1150
                     /locus_tag="VHS97_22780"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX3451098.1"
                     /translation="MNGTKRITATALIVVSLAASCLAQDQPKKSWVWLTKQGVWGYGY
                     QIDEGPNRGLWRIDPETKRAPEEMVPTTDPYGFAAILNQYRAAAGLAPLVYDHELSAW
                     AAQNNVVQSEKGIGHHITANCFQNCAWNTPDAASTADEWMNSRGHRANMLTPAASRFG
                     IAYGPGPYWTMNAR"
     gene            complement(1331..2668)
                     /gene="thiC"
                     /locus_tag="VHS97_22785"
     CDS             complement(1331..2668)
                     /gene="thiC"
                     /locus_tag="VHS97_22785"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX3451099.1"
                     /translation="MTQIEMARQGIVSLEMEHVARRESLLPELIRSEVARGRMIIPAN
                     TVHLRLGLEPMAIGVKAKCKINANIGNSAVTSDIENELSKLRMAVELGSDTVMDLSTG
                     GNIDAIRQEIIAASPVPVGTVPLYQAIQEVKKVEELSATDLLDMVEHQAKQGVDYMTI
                     HAGILRDYIPLTAHRLTGIVSRGGSLMAQWMFARDQENPFYTHFDELCDIMREYDVSF
                     SLGDSLRPGSIADASDAAQFAELKTLGELTRRAQARGCQVMVEGPGHVPMDQIAMNME
                     KQAIECNEAPFYVLGPLVTDVAPGYDHITSAIGAALAGWHGASMLCYVTPKEHLGLPE
                     VEDVRQGVIAYKIAAHAADLARHRPGARDRDDALSRARYSFNWEEQFRLSLDPETARR
                     MHDETLPQDAFKTAAFCSMCGPKFCSMRITEDIRKQAAATASLVQLEPAGAGA"
     gene            2909..3313
                     /locus_tag="VHS97_22790"
     CDS             2909..3313
                     /locus_tag="VHS97_22790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451100.1"
                     /translation="MNRLAAHDRILESTAEELDLACGVLSQKASALACEARQMSKSHF
                     IPCSHADCRAQASYKIAARWSDGNLAELKTYGFACSEHIGAVFRDSEQRRREYVPSPG
                     ESIEELAIYRYEPGKRDRFLQRLWGLEENYRS"
     gene            3567..4046
                     /locus_tag="VHS97_22795"
     CDS             3567..4046
                     /locus_tag="VHS97_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HEX3451101.1"
                     /translation="MNILTTRFGLIQACETELILVPEGLLGFRAFTHYIHLPDPVVDG
                     LSWLQSVTAADLAFGLVAPPLAVSDYRVELRQADRAALELDDERSAMIYVILNRGEGG
                     VLTVNLQGPVVFNPIRRLGRQLVLTSSRYPVRYPLESCSSAPAPTLLPGPSPLRATA"
     gene            4180..>4436
                     /gene="csrA"
                     /locus_tag="VHS97_22800"
     CDS             4180..>4436
                     /gene="csrA"
                     /locus_tag="VHS97_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX3451102.1"
                     /translation="MLVLSRHRDESIIIGDDIVITVVDIRGDKVRLGIAAPIEISVHR
                     QEVYEAIQRENRQASRLEPHDARQIDRLNPPPRREPRRTRG"
BASE COUNT          856 a         1364 c         1337 g          879 t
ORIGIN      
        1 gcaactgatc gaagcattcg tttcggccgc ggcaacaagc aagagtatgc cggcacttgc
       61 caaggtcggc tgacagccgc ggcccaaccc cggtgcctcg ttgttggcgg cgttcctgtc
      121 tggggaaacc ctgctgggaa cgccgctttt tcatttcgaa cgacccccga cggtgactca
      181 cggagccggc tgccttgtgt gttgatttta cagcgatctc gcttgtaaaa agcgacctct
      241 cgggccggct ctgtatttag ttgcgccccc gcacgatcga tacgaaccga acggtcgccg
      301 cacggactgg ggctagacca ctcaattctg gtcgaaaatc ggttcggcgc gcgacttgct
      361 ttaccagccc accatcggcg gatccgccga ttcttgattc ccgaggcgcc ggatcttcgc
      421 ggccattcct cggataaata gatcggtctt cagtaagaca tccctgcgcc tccacaggac
      481 cacggaaggg gcgcggctct gtggctcgac tcttttgcga gccgcggtcg ccgtccgcgc
      541 ggattcccca cggatcggtt cgccctgcct ggttgcccag ggtttccact agcatacgcg
      601 agctcaacga aaaaaaggga aaccgatgaa cggcacgaaa cgcatcaccg cgacggcctt
      661 gatcgtggtt tcactggcgg cgtcctgcct ggctcaggac cagcccaaga agtcttgggt
      721 ttggctcacc aagcaaggcg tctggggcta cggctaccag atcgacgagg gccccaatcg
      781 gggtctgtgg cggattgacc ccgagacgaa acgggctccc gaggaaatgg taccgactac
      841 cgacccgtat ggattcgccg ccatcttgaa ccagtaccgc gccgccgccg gactggcgcc
      901 gttggtctac gatcacgaat tatcggcgtg ggccgcccag aacaacgtgg tgcagtcaga
      961 aaaaggtatc gggcaccaca tcacggcgaa ttgttttcaa aactgtgcct ggaacactcc
     1021 cgacgccgcc agcacggccg acgagtggat gaactcgcgc ggccaccggg caaacatgct
     1081 cacaccggcg gcatcccgct tcggcattgc gtacggtccg ggtccctact ggacaatgaa
     1141 tgctcggtga atcccgagtc gcggcatgcc gcgcatttct atgcactctt gaaactggac
     1201 cccgggattt cggacattgc caatcacggc gtggcgcacg accggtcgcc agccagggac
     1261 cacgcaatcc ggaaacccgg ggtcatctcc tgcacagcga caaccaattc tccctcggcg
     1321 agccccctct tcaggcgccg gcgcccgccg gctcgagctg aacgagcgac gccgtggcag
     1381 cggcttgctt gcgaatgtct tccgtgatgc gcatcgagca aaacttgggt ccgcacatgc
     1441 tacagaatgc cgcggtcttg aaggcgtctt gcggcagcgt ctcgtcgtgc atccgccggg
     1501 cggtctcggg atccagtgac aagcggaatt gttcctccca gttaaagcta taccgcgccc
     1561 gcgataacgc atcgtcgcgg tcacgagcgc ctgggcgatg ccgcgccaag tctgcggcat
     1621 gtgccgcaat cttgtaggcg atcaccccct ggcgaacatc ctcgacttcg ggcaaaccca
     1681 ggtgctcttt gggcgtcacg taacagagca tactggcgcc atgccagccg gccaatgccg
     1741 cgccgatggc cgaagtgatg tgatcgtatc ccggcgcgac gtcggtcacg agcgggccca
     1801 acacgtaaaa tggggcttcg ttgcattcaa ttgcctgctt ctccatgttc atggcgatct
     1861 ggtccatggg cacgtggcca gggccttcca ccatgacctg gcagccgcgg gcctgggccc
     1921 gtcgggtcag ctcacccaat gtcttcaact cggcgaactg ggcggcgtcg gacgcgtcgg
     1981 cgatgctgcc gggccgcagg ctgtcgccca ggctaaatga cacgtcatat tcgcgcataa
     2041 tgtcgcacag ctcgtcgaag tgcgtgtaaa atgggttttc ctgatcgcgc gcgaacatcc
     2101 attgggccat gagcgagccg ccgcgcgaga cgatgcccgt caggcgatga gccgtcaaag
     2161 ggatgtagtc gcgcaagatg cccgcgtgga tcgtcatata gtcgacccct tgcttggcct
     2221 ggtgttccac catgtcgagc aggtcggtcg ccgaaagttc ctcgaccttt ttgacttcct
     2281 ggatcgcctg atacaagggc acggttccta ccgggaccgg gctcgcagca atgatttctt
     2341 ggcggattgc gtcgatattg cccccggtcg acaggtccat cacggtgtcg gagcccagtt
     2401 caacggccat acggagcttg gaaagctcgt tttcgatgtc ggaggtgacc gccgaattgc
     2461 cgatgttggc gttgatcttg catttggctt tgacgccgat ggccatcggt tccaatccga
     2521 gcctcagatg gacggtgttg gccgggatga tcatccgccc ccgcgccact tccgaccgga
     2581 tcaactcggg taggaggctc tcgcgccgcg caacgtgctc catctccaac gaaacaatcc
     2641 cctgccgagc catttcgatc tgcgtcatgg tgcaagcctc tgaaaatcat tcgatcgcgg
     2701 ccgcgaaaat cttggcaccg gaaaaccggt tgctaagtag aagacttcgt gctcctcttt
     2761 tttataacaa tgtccgcagc aatcggtaag gtggaacggt cctctgccac aggttgaaca
     2821 tggttgcaag gcgctgcgga catgactgag gcgatcaaag gacgagtgcg caatgtgaat
     2881 tgtcgggggg tcggcgagat cgtcaggttt gaaccgactc gccgctcacg atagaatcct
     2941 ggaatcgacc gccgaagagc ttgaccttgc ttgcggagtt ttgtcgcaaa aagcttcggc
     3001 gctcgcgtgc gaggcccggc aaatgtccaa gagccacttc attccttgct cccacgctga
     3061 ctgccgggcg caggcctcgt acaaaattgc cgcgcgctgg agcgatggca acctcgccga
     3121 gctgaagaca tatggttttg cctgctccga acatatcggc gccgtctttc gcgattccga
     3181 gcagcgtcgc agagagtacg tcccttcccc gggcgagtcg atcgaagaac tggcaatcta
     3241 tcgctatgag ccgggaaaac gcgaccgctt tctgcaacgg ttgtgggggc tggaagagaa
     3301 ttaccggtca tgagggcgcc gtcggcggcg agacggctgc ggccacgaca aagtggcgct
     3361 cataaccggc acacgcgagc cagaggttag ttctacctcc gttacgaacg gagcgactgg
     3421 cgcagccatc gcgaggcgta ctgcgcttgg atttccgtcg acagggactc ttccccgtcg
     3481 agtggacggg acgatataaa ggtcggtaaa gctgctcccc tcggtcagcg attatgtttg
     3541 gatcagttca aggaggtaac caggaagtga acatcctcac gacgcgcttc ggcctgatcc
     3601 aggcttgtga aacggagttg atcctcgttc ccgaaggctt gcttgggttt cgcgcgttca
     3661 ctcattacat ccatctgccc gacccggtcg tggacggtct gtcgtggttg cagagcgtca
     3721 ccgccgcgga cctggcattt ggactggtcg cgcccccgtt ggcggtgagc gactatcggg
     3781 tcgagctccg ccaggcagac cgggcggcac tcgagctgga cgacgagcga tcggcgatga
     3841 tttatgtgat tctaaatcga ggtgaggggg gagtgctgac ggtcaattta caagggccgg
     3901 tggtcttcaa cccgatccga cgcctgggcc ggcaactggt tctcacctcc agtcggtatc
     3961 cagtgcggta tcctcttgaa tcgtgttctt ccgccccggc accaaccctg ttgcctgggc
     4021 cgtcaccgct gcgagcgacg gcgtaacccg ctctagcaga gcagcatgag tcttattggc
     4081 aacgtaccat ccccagctcc gccgtggtca ccaagtgccc gggcaacgtg atcagggacg
     4141 gtcgcgggcc tcgctggcag gaggaaggag ccgaatacga tgctggttct gtcccgtcac
     4201 cgcgatgaaa gtatcatcat tggcgatgac atcgtcatca ccgtggtgga tattcgcggc
     4261 gataaggttc gattggggat cgccgccccg atcgagatct ccgtgcaccg ccaagaagtg
     4321 tacgaggcca ttcagcgcga gaatcgccag gcgagccgac tggaacctca cgatgcccgt
     4381 cagatcgatc gcctcaatcc gcccccgcgg cgcgagccgc gccggacgcg cggcga
//
LOCUS       DATAQL010001323         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1660501, whole genome shotgun sequence.
ACCESSION   DATAQL010001323 DATAQL010000000
VERSION     DATAQL010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660501"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VHS97_22805"
     CDS             complement(<1..1034)
                     /locus_tag="VHS97_22805"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX3451103.1"
                     /translation="MKSNVRWFAATIILVLPLLGSQAHAAGSADSVVKVFSQMRLPNP
                     VRPWTKQNPIEAMGSGVVIERNRILTNAHLVLYADEIFVQGGQGGDRVEAKVAAIGPG
                     IDLAVLTLEDDAFFAKRPAIARASKLPEITSRVAVQGFPIGGTSLSTTQGTVARIEYD
                     IYETGAAGLRIQVDAVINPGNSGGPAVVDGKMIGVISGHAENIGYIIPNKEIDDFLTD
                     VADGHYEGKPFAFDQFQTMENEALRGKLGVAKNVHGLMVREPKHRDPSYPLKEFDILS
                     RIGTHEIDNEGMIQAEDNLRLSFMSQVPKLAKNGTVPVSVLRAGKTIEVALPVSREND
                     HLLRSYDGRYP"
BASE COUNT          301 a          473 c          451 g          315 t
ORIGIN      
        1 ggataccggc catcgtagct gcggagcaaa tggtcattct cgcgactgac tggcagggcc
       61 acctcaatcg tcttaccggc ccgcaacacg cttaccggca cggtcccatt cttggcgagc
      121 ttgggcacct gcgacatgaa cgagaggcgg agattgtcct ctgcctgaat catcccctcg
      181 ttgtcgatct catgcgtacc gatccggctc agaatgtcga attccttgag cgggtacgaa
      241 ggatcgcggt gcttgggctc gcgaaccatc agtccgtgca cgtttttggc cacgccaagc
      301 ttgccgcgca gtgcttcatt ttccatggtc tggaactggt cgaaggcgaa gggcttgccc
      361 tcgtagtggc cgtcggcgac atcggtcagg aagtcgtcaa tctccttatt ggggatgatg
      421 tagccgatgt tctcggcatg gcctgagatc acgccgatca tcttgccatc aacgacggcc
      481 ggcccaccgc tgttgccggg gttgatcacg gcgtcgacct ggatccgcag gcccgccgcg
      541 ccggtctcgt agatatcgta ctcgatgcgc gccacggttc cctgagtggt cgagagactg
      601 gtgccgccaa tcgggaagcc ctggacggcg acccgcgacg tgatttctgg gagctttgaa
      661 gcgcgggcga tggcgggccg cttggcgaag aaggcatcat cctcgagtgt cagcacggcc
      721 aggtcgatcc cgggcccgat ggcggccacc ttggcctcga ccctgtctcc cccttgcccg
      781 ccttgaacga agatctcatc ggcgtacaga accagatgcg catttgtcaa gatccggttc
      841 cgctcgataa ccacgccgct acccatcgcc tcgatggggt tttgcttggt ccacggacgc
      901 acggggttgg gtagccgcat ctggctgaag actttgacga ccgaatcggc tgaacctgct
      961 gcgtgggcct gggagcccaa aagtggtaag acgagaataa ttgtcgccgc gaaccaccgg
     1021 acgttgcttt tcatgatgct tgtttcctct cgtcgatcgg accttgagac tgactcgtac
     1081 ccatcagccg tacgcacgaa ggaccgagac cttacagttg cgccgtggat tgacgcggga
     1141 agtgccagca gaccggatcg tgcaatccac gtcgggaacc cttgcgcgat cgtattgatt
     1201 tagcgctttt tgacttcgcc cctaccttcc agacatatgt ctgactgcct cctcggcaca
     1261 gaaaaaacgc ggtcggacat atgtttcgga aggctgatcc ggaccatatg ttgtccagcg
     1321 gcacgacata tgtcgggatc aacaggggcg gaatcataaa tcccggcgtg tgaaccacgg
     1381 gacgagatca gacaccgatc ccgggacggg ataatacgcc gcccccccga gaggaaattg
     1441 aacgccgacc cgcgccgtat tgcatcgacc caccattcaa ccggcgacgc gctcatcggg
     1501 ctggtaatcg aaagaccgct cccctgcccc aacagcgtcg
//
LOCUS       DATAQL010001324         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4258132, whole genome shotgun sequence.
ACCESSION   DATAQL010001324 DATAQL010000000
VERSION     DATAQL010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4258132"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VHS97_22810"
     CDS             complement(<1..249)
                     /locus_tag="VHS97_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3451104.1"
                     /translation="MPIPSHTVDTGIAVGTDELTKSYGSATSPVYALRGVTLEVARGE
                     RIALLGKSGSGKSTLLNLLGGLDRATSGRLRVAGHEVDR"
     gene            589..1572
                     /locus_tag="VHS97_22815"
     CDS             589..1572
                     /locus_tag="VHS97_22815"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX3451105.1"
                     /translation="MSKKVLVTGAAGFIGSHLTERLVRDGHQVRAFVRYNGRDDRGNL
                     ADLPADIQAAIEVERGDLKDPGAVKRAVEGREWVFHLGALIAIPYSYHNPYDVVQTNV
                     LGTAHVLDACRASSTLARVVLTSTSEVYGTAQTVPIDERHPLQGQSPYAATKIGADAL
                     GESYHRAFGLPVAILRPFNTFGPRQSARAIIPTIISQALVRPKIKLGRLDPRRDLTYV
                     KDTAAGFVAIAGCDRALGRAVNIGRGEDIAIGELVETIARRLGRPIAVETDQERYRPA
                     ASEVERLLAGTALAQSLWGWKPRYSLEDGLDETIAWVRDHLDRFRIDAYTT"
     gene            1659..2645
                     /locus_tag="VHS97_22820"
     CDS             1659..2645
                     /locus_tag="VHS97_22820"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX3451106.1"
                     /translation="MTIDWTPLADLIETNDRFLVTTHVRPDGDALGSEVGMAGLLRQK
                     GKDVRVVNASPTPPRYDFLDPDSTLFEHFGHKVQPEELADRQVAVILDLSSWNQLGDM
                     ASFIRQFPGSRVVVDHHVSEDDLGARVFKDSTAEATGILVMKAIAALGGSLTPEVANA
                     LLTAIAMDTGWFRHSNTRSRTLRAAAELVDAGAKVDGLYRQLFERNTLSRVKLMGETL
                     GGMKTDLGGKIAYATISKDDLHRTKAIPPDSEDLVDFTVSLRGVEVGMLFIEQARGGV
                     KVSFRARNGLDCSLLAAKFGGGGHREAAGATISGTMPEVVDRVLETVRKAVG"
BASE COUNT          572 a          857 c          891 g          523 t
ORIGIN      
        1 ccggtcgact tcatgccccg cgacgcgcag ccggccggac gtcgcgcggt caaggccgcc
       61 gagcaggttg agcagcgtcg attttccaga acccgactta cccaatagag cgatgcgctc
      121 gccgcgcgcc acctccagcg tcaccccacg cagggcgtac acgggcgagg tagccgaacc
      181 gtacgacttg gtgagttcat ccgtccccac ggcgatcccc gtgtccacgg tgtgagacgg
      241 aatgggcacg atccttgtct ccgaccagcg aatcttacat ctgtcgcacg taagaaacat
      301 cctagcacac cagtgggatg aaaaaagggg cctggtcttg gggtctggat tcgggagcca
      361 ctcgaccggg tgaactttct catcattcca ggtcacgcat aagtttgacg cagaatcggt
      421 ttgatagcac gaacacccga gaagacttcc acgagatcgt ctggggcgat ggtcgattct
      481 gggacttatt cggtgtagcg aagaacgcca aaatgcctta cgatctgcga ccgaagaatc
      541 agtcgaaaac cgtgacgatt cgaggcgtgg caaaggagtg gcacgtcgat gagtaagaaa
      601 gtcctggtga cgggggcagc gggcttcatc ggcagtcacc tgaccgagcg gctggtccgg
      661 gatggccacc aggtgagggc gttcgtacgt tacaacggac gtgatgaccg cggaaatttg
      721 gcggatttac ctgccgatat tcaagcagcc atcgaggtcg agcgcggcga tttgaaagat
      781 cccggcgctg tgaagcgtgc ggtcgagggg cgagaatggg tctttcatct cggtgcattg
      841 atcgcgatcc cctactcgta tcacaaccca tacgacgtgg tgcagactaa tgtcctgggg
      901 accgcgcacg ttttagacgc gtgtcgagca agctccacgt tggccagggt agtgctcacc
      961 tcgacgtccg aagtttacgg caccgcccag accgtcccca tcgacgaacg gcacccgctc
     1021 cagggtcaat cgccctacgc ggcgaccaag atcggggccg atgcgctcgg ggagagttat
     1081 catcgggcgt tcggcctgcc ggtggctatc ttgcgaccgt tcaacacttt tggaccgcgg
     1141 cagtcggcgc gggcgatcat ccccacaatc atcagccagg ccctggtgcg gcctaagatc
     1201 aagctcggcc ggctagaccc gcgccgggac ctgacctatg tcaaagatac ggccgccggc
     1261 ttcgtcgcca ttgccggctg tgatagagcg ctgggcagag ccgtgaatat tggccgcggt
     1321 gaagacatcg ccatcggcga actggtcgaa acgatcgccc gccggctggg tcgaccaatc
     1381 gccgtcgaaa ccgatcaaga gcggtaccgg ccggcggcga gcgaggtcga acgactactc
     1441 gcaggcacgg cgcttgccca gagcctctgg ggttggaagc cgcgctactc gctcgaggac
     1501 ggcctcgacg agacgattgc ctgggtccgc gatcatcttg accgcttccg gatcgatgct
     1561 tatacgacct gaccggccgg cgttgccaat ccagcggcgg ggtcgctacg ctgtttctac
     1621 acacgatgac acgcaccgac cgtcacgcgg ggctgaaaat gacgatcgac tggacaccgc
     1681 tggctgacct gatcgagacc aacgaccgtt tcctggtgac cacgcacgtt cggccggacg
     1741 gagacgccct gggctcagag gtcggtatgg ccggactgct ccgccagaag gggaaggacg
     1801 tacgggtggt caacgccagt ccgacgccgc ctcggtatga cttcctcgat cccgactcga
     1861 ccctgttcga gcattttggc cacaaggttc agcccgaaga actcgccgat cgtcaggtcg
     1921 ccgtcattct cgacttgtct tcatggaacc agctcggaga catggcaagt ttcatccgcc
     1981 agtttcctgg ttcccgcgtg gtcgtcgacc accacgtgag cgaagacgac ctgggcgcgc
     2041 gggttttcaa ggattcgacc gcggaggcaa cgggcatcct cgtcatgaag gcaatcgcgg
     2101 cactcggggg ttcattgacg ccggaagtcg ccaacgcctt gctgacggcg atcgcaatgg
     2161 atactggctg gtttcgtcat tccaacacgc ggtcgcgaac gctccgggca gccgccgagt
     2221 tggtcgacgc gggagccaag gtcgacggac tctatcgcca gctcttcgag cgaaacacgc
     2281 tcagccgcgt caagctcatg ggagagacac tgggcggcat gaagaccgac ctcgggggga
     2341 aaatcgcgta cgcgacgatt tccaaagatg atctgcaccg caccaaggcg attccgcccg
     2401 actcggagga cctggtggac ttcaccgtca gccttcgcgg tgtcgaggtg ggcatgctct
     2461 tcatcgaaca ggctcgcggc ggcgtgaagg tctcgttccg ggctcgcaat ggcctcgact
     2521 gttcgctctt ggccgccaag ttcggcggtg gcggccatcg cgaagcagcg ggagcgacga
     2581 tatctggcac catgccggag gtcgttgatc gggtgcttga aaccgtgcgc aaggccgtag
     2641 gttaataagg tcgtaggtta atgcaactgc ggcagttgag ccctccgagc gctcaaggat
     2701 tgacgattcg ctggactcgg ccgtcaccac ggcgccgtcg aacgaccggc gacacgtgga
     2761 aattcatgga caaggcacta tagtaatcta ggaaatgatg gcgtttttgg tgaacgcctg
     2821 cctccccgcc tgcgaatcga tcc
//
LOCUS       DATAQL010001325         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1205960, whole genome shotgun sequence.
ACCESSION   DATAQL010001325 DATAQL010000000
VERSION     DATAQL010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205960"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /gene="lpxB"
                     /locus_tag="VHS97_22825"
     CDS             complement(<1..1150)
                     /gene="lpxB"
                     /locus_tag="VHS97_22825"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX3451107.1"
                     /translation="MRIFISAGEPSGDLHAANLISSLRDCFPTARFTGFGGERMAAAG
                     ADLLYPLANLAVMWFLNVFLNLITFIRLIFVADRYFRDKRPDAVVLVDYPGLHWWIAG
                     RAKARGIPVFYFVPPQIWAWGGWRVKKVRKYVDKVLCSLPFEPAWYHARGVTEAVYVG
                     HPYFDELADRPLDDRFLADETGRSSALVAILPGSRTQEVTRNLPDMVRAASKLAQKHT
                     DVRFAVACLHERHKKLAEQIIAQTIGRESTSPGPVIEVYSERTPELIRLARVAWAVSG
                     SVGLELMVEGLPSVVLYKVNRFDLWVARHFIKSRYISLVNLLADDEVFPEYLTWHDVS
                     DEMVRWAVRWLDDSETRQRTVDLILNVRQGVAQPGASKRAATHIAHWLF"
     gene            1526..>4068
                     /locus_tag="VHS97_22830"
     CDS             1526..>4068
                     /locus_tag="VHS97_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3451108.1"
                     /translation="MTLLAAGQTAMTRSTVERADAIFREQLLAEYQRVDRIFAFLLTA
                     EWLSAILVALMISPYAWAGETASIHIHVWTAIILGGAIVSLPVALALLSPGTSMTRHA
                     VGAGQMLLSALLIHLWGGRIESHFLIFVSLAFLAFYRDWKVLVTASALVALDHYLRGY
                     FWPRSVYGVRAVAAWRWAEHSGWVVFEDIVLVRGCLQSLREQWALAFRQAEVEASHAR
                     VEDTVAERTALLRRSNESLRIEVQERRRAENALRQSDAKARKLALVASHTRNSVILAD
                     AQGRIEWANDAFTSLTGYTLAEVIGRTPGSFLQGPRTDRETITLMRKKIHSGQGFVVE
                     ILNQNKSGRPLWLLVEAQPIHDGSGAVTQFIGVQEDITARKQAEWRLTAQHNAMRVLA
                     ESVSLDEAIPQLFRVIALGLGLDRGEFWQGEASAGMIRMTHDWSAAPAEDLDFAAGSR
                     AIYLARGDSLTGRVWALGRLERIEDVAHDANFLRSEMAELAGFRAAVGIPVLCGTEVI
                     GVMIFLNRTALEIDRSLHDLLLALGHQIGMFIERRRAEDKFRVIFEQSSDAHLLFHEK
                     DGIIDCNEAALHMTGCRDRRLLLGIHPASISEEFQPDGRRSMDKCLEMDAIARRDGHH
                     RFDWWIRRMDDGEVFPCEVTLTPVEVAGYSVLLVVLHDLTERKQHEEVINEAKLAAEA
                     ASRAKSEFLAFMSHEIRTPMNGIIGLTELALDTQLTARQREYLGLVKSSADSLLAVIN
                     DILDFSKIEAGKLDLDPTPFSLRDAIDETLQALALRAHAKGVELVCRPAPQVPDTLVG
                     DAGRLRQVLVNLVGNAIKFTELGEILVSVGLDHVGTEELVLRFAVADTG"
BASE COUNT          787 a         1213 c         1210 g          858 t
ORIGIN      
        1 cgaagagcca gtgagcgatg tgcgtggcgg cgcgcttcga agcgccgggc tgggccacgc
       61 cctgccgcac attcagaatc aaatcgactg tccgctgtcg tgtttctgaa tcgtcgagcc
      121 accttactgc ccaacgaacc atttcgtccg agacgtcatg ccacgtcaga tattcgggaa
      181 acacttcgtc gtcagccagg aggttcacca aactgatata tctcgacttg atgaaatgtc
      241 gcgctaccca gagatcgaaa cgattcacct tgtagagcac gaccgacggg agtccttcga
      301 ccattagctc gagtccgacc gaccccgaga ccgcccatgc aacccgcgcc agcctgatca
      361 gctcgggagt gcgctccgag tagacctcga tcaccggacc gggcgacgtg ctttcccgac
      421 cgattgtctg ggcgatgatt tgctcggcga gctttttgtg gcgttcgtgc aggcacgcga
      481 ctgcgaagcg cacatccgta tgtttttgag cgagcttcga cgccgctcgt accatgtcgg
      541 gaaggttgcg cgtcacttcc tgcgtccgtg agccgggaag gatcgccacg agcgcactag
      601 agcgcccagt ctcgtcagcc aggaagcgat cgtccagagg ccgatcggcc agctcgtcaa
      661 agtacggatg cccgacgtag actgcttcgg taacgccacg cgcgtgatac cacgctggct
      721 cgaagggaag gctgcacaag accttatcaa cgtacttgcg aaccttcttc acccgccagc
      781 cgccccaagc ccagatctga gggggaacga agtaaaacac cgggatgcca cgtgccttgg
      841 cgcggcccgc tatccaccaa tgcaaacccg ggtagtcgac cagtactacg gcatcagggc
      901 gtttatcgcg aaagtaacga tcggccacaa agatcaatcg aataaacgtg atcaaattca
      961 agaagacatt gagaaaccac atcaccgcaa ggtttgcgag cggatagagc aggtctgcgc
     1021 cggccgctgc catcctctca ccaccgaatc cggtgaatcg ggcagtcgga aaacaatcgc
     1081 ggagcgagct gatcaggttg gcagcgtgca gatcgccact gggctcaccc gctgagatga
     1141 agatacgcat tgggactcgc ttccttgctc agtgctggcc gtatcaatcg ttccctcgag
     1201 ccgccgtgag cctatcaaat ggtgttgttc ctgaaaagac gcaatgcgac cggtcgccgg
     1261 tagccatcta atgtcatcat tccactggga taatctttcg cgccgtgagc agttccgctc
     1321 acctgtcgtc gcacaaatcc tatgcttacg gtagaagcaa ccctcccgcc gaatgatcga
     1381 gtgtcgttgg gaatccttaa gtgcttacta tgtcaacggt tgggcattca gcgttcggtc
     1441 ttcggacaat ctaggggctc gtcgtgggat ccggtctttt cttcctgtca gctttcaacc
     1501 cgcccggcgg cggggagtcg cggcgatgac gcttttggcg gccgggcaga ccgcgatgac
     1561 gcggtcaact gttgagagag ccgacgccat tttccgagag cagttgcttg ccgaatacca
     1621 acgggtcgat cggattttcg cgttcttgct cacggcagag tggctgtcag ccattctcgt
     1681 cgctctgatg atttcccctt acgcctgggc tggcgagacc gcttcaattc atatccacgt
     1741 ctggactgcg attatcctgg gcggcgcgat cgtcagcttg ccggtcgctc tggctttgct
     1801 cagccccggc acgtcgatga ctcgccacgc cgtcggggcc ggtcagatgc tgttgagcgc
     1861 tcttttgatc catctctggg gggggcggat cgagtcccat ttcctcatct tcgtctcgct
     1921 tgccttcttg gctttctatc gtgactggaa agttctcgtc acggcatcag cgcttgtagc
     1981 cttggaccac tatctccgcg gatacttctg gccgcgttcg gtctacggcg taagggcggt
     2041 tgctgcctgg cgctgggccg aacactccgg ctgggtcgtt ttcgaggata tcgtgcttgt
     2101 ccgcggctgc cttcagtcgc tccgcgagca gtgggcgctt gcctttagac aagcggaagt
     2161 cgaggcatcc catgcccgtg tggaagacac ggtcgcagaa cgtaccgccc ttctgcgacg
     2221 gtccaatgag tcgctgcgca tcgaagttca agagcggcgc cgcgccgaga atgccctgcg
     2281 tcaaagcgac gcaaaggctc gcaagctggc tttggtggct tcccatacgc gtaacagcgt
     2341 gatcttggcc gatgcgcagg gccggatcga gtgggccaac gatgctttta cgagcttgac
     2401 cggctacacg ctcgccgaag tgatcggccg gacgcccggc tcctttcttc aagggccgag
     2461 aaccgaccga gagactatca ctttgatgcg gaaaaagatc cactccggcc agggattcgt
     2521 cgtcgagatc ttgaatcaga acaagtccgg tcggccattg tggctcttgg tcgaggcgca
     2581 gcctatccac gacgggtcgg gtgcggtgac ccagttcatc ggtgtccagg aggacattac
     2641 cgcgcggaaa caagcggagt ggcgattgac ggcccagcac aacgcgatgc gcgtcttggc
     2701 cgagtcggtg tcgctggatg aagcaatccc tcagcttttt cgcgtcatcg cgctagggtt
     2761 gggcctggat cggggcgagt tctggcaggg cgaagcgagc gcgggcatga tccgaatgac
     2821 ccacgactgg tcggccgctc cggcagagga tcttgatttt gcggccggtt cgcgcgcgat
     2881 ctaccttgct cgaggcgaca gtctcacggg ccgcgtctgg gcgctcggtc gactcgagcg
     2941 tatcgaagac gtcgctcacg acgccaattt cctgcgctcc gagatggcag agctcgccgg
     3001 gtttcgcgcc gccgtcggca tacccgttct gtgcggcacg gaggtaatcg gcgttatgat
     3061 ttttcttaac cgaactgctc tggagatcga ccggtcgctt cacgatcttc ttctggctct
     3121 tggacatcag atcggcatgt ttatcgaacg tcggcgggcc gaggacaagt tccgcgtcat
     3181 atttgagcag tcgtccgatg cccatctgct attccacgaa aaggacggca tcattgactg
     3241 taatgaagcc gcgctgcaca tgacgggttg tcgcgacagg aggcttttgc tgggcataca
     3301 tccggcatcg atatcggagg agtttcaacc cgacggccgc cgctcgatgg ataagtgcct
     3361 tgagatggat gccattgcgc gccgcgacgg acaccatcgg tttgactggt ggatccgccg
     3421 gatggatgac ggcgaggtct tcccttgcga ggtgacgctc acgccggtcg aggtcgcagg
     3481 gtactcagtc ctgcttgttg tcctgcacga cctgaccgag cgaaagcagc atgaagaggt
     3541 gatcaatgag gcgaagctgg ccgccgaggc cgccagccgc gccaagagcg aattcctcgc
     3601 cttcatgagc cacgaaatcc gcaccccgat gaatgggatc atcggcttga ccgagttagc
     3661 actggatacg cagctcacgg cccggcaacg tgagtatctt ggcctcgtaa agagctcggc
     3721 cgattccctg ctcgccgtga tcaacgacat actcgacttt tccaagatcg aggcgggaaa
     3781 gctcgacctt gacccgacac ctttttccct ccgcgatgcg atcgatgaaa ccctccaggc
     3841 cctggccctg cgcgcgcatg ccaagggcgt cgagctggtc tgtcggcccg caccccaggt
     3901 tccagacacc ttggtcggcg acgctggccg gttacgccag gttttggtca acctggtcgg
     3961 caatgcgatc aaattcaccg aactgggtga aatcctggta tctgtcgggc tcgatcacgt
     4021 gggaactgag gaacttgtac tgcgctttgc agtggccgac acgggaat
//
LOCUS       DATAQL010001326         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_747803, whole genome shotgun sequence.
ACCESSION   DATAQL010001326 DATAQL010000000
VERSION     DATAQL010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747803"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VHS97_22835"
     CDS             complement(<1..443)
                     /locus_tag="VHS97_22835"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3451109.1"
                     /translation="MPENGVKKYIVRLSADEREALEALISKGKRPASLILKARILLKA
                     DISDAGEGWSDGRIIKALDTSATTVYRTRQRLVEEGFDAVLRRKPRERPSIPRIFDGE
                     SEARLITLACSEPPAGHAKWTLRLLEEKVVELGIVPRASDNTIGWT"
     gene            616..1341
                     /locus_tag="VHS97_22840"
     CDS             616..1341
                     /locus_tag="VHS97_22840"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3451110.1"
                     /translation="MMAAPRRVIEIPIGAIERIELEAIARSRTEWACRAQRARILLAY
                     HADPSTYAVGETVGVTHQTVQRCLDRAVRFGVMAALDDSPRPGREPTITDDAKAWVVS
                     LACQKPKELGHPHEVWTTRLLARHIREHAEAAGHRCLTHLAQGTVCKILDKRDVQPHK
                     VRYYLERRDDAFEQKMAEVLCVYREVAVMRENEPAQPNVAIISYDEKPGIQAIGSTAP
                     ACRRVRDITGDGRATMNTSATAR"
     gene            1344..>1567
                     /locus_tag="VHS97_22845"
     CDS             1344..>1567
                     /locus_tag="VHS97_22845"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3451111.1"
                     /translation="MLAGIDLLTGKVHGCVENRHRSREFIAFLQKIDAAYSTDTAIKL
                     ILDNHSAHISKETKTWLAAQPDGRFTLVFTP"
BASE COUNT          312 a          484 c          483 g          288 t
ORIGIN      
        1 gtccacccga ttgtgttgtc actggccctg ggcacgatcc cgagttcaac gaccttctcc
       61 tcaagcagac gaagggtcca tttcgcatgg ccggccggtg gttcggagca ggcgagtgtg
      121 atcaatctgg cttcgctctc accgtcgaag atgcggggga tcgagggtcg ctcgcgaggc
      181 ttgcggcgca gcaccgcatc gaacccttct tcgaccagcc gctgacgtgt tcggtaaacc
      241 gtggtggcgc tggtatcgag tgccttgatg atacggccgt cgctccaacc ctcgccggca
      301 tcggagatgt cagccttgag caggatccga gctttgagga taaggctggc gggacgtttg
      361 cccttgctga tcagagcttc aagcgcttct cgctcgtccg cgctcaggcg aacaatgtac
      421 ttcttgaccc cgttttctgg cacgacccga atcccggcag cgttgacaac gttgccatag
      481 ggtcatggga ccccgtcaat ttcaatgacg caagccacta gtacagcgat tcggtggaac
      541 cgaccggttg gggcagattc tcatcttggg catcatatgc tgtaatccgc caaagatcac
      601 ggacgacggc agggcatgat ggcagcacct cggcgggtca ttgaaatccc gatcggagcg
      661 atcgagcgca tcgaactgga ggcaatcgct cgatcgcgaa cggaatgggc ctgtcgagcc
      721 cagcgggcac gcatcctgct ggcctatcac gccgatccat cgacttacgc ggtcggcgag
      781 acggtcggtg ttacccatca gaccgtgcag cgttgcctgg atcgcgcggt gcggtttggg
      841 gtgatggcgg cgctcgacga cagcccccgg cctggcaggg agccgacgat tacagacgac
      901 gccaaggcat gggtggtgtc gttggcgtgc cagaagccga aagagctggg acacccgcac
      961 gaagtttgga cgacacggct gctggcgcgt cacatccggg agcatgccga ggcagcaggg
     1021 catcgctgcc tgacacacct tgctcaaggc accgtttgca agatcctcga caagcgcgac
     1081 gtgcaaccac acaaggtccg ctactacctt gaacgccgcg acgatgcgtt cgagcagaag
     1141 atggcagagg tgctgtgcgt gtatcgcgag gtcgctgtga tgcgcgagaa cgaacctgcc
     1201 cagccgaacg ttgcgatcat ctcctatgac gagaagccgg gcatccaggc aatcggcagt
     1261 accgccccag cctgccgccg cgtccgggat atcacagggg atgggcgcgc gaccatgaat
     1321 acaagcgcca cggcacgcta agcttgctgg ccgggatcga cctgctcacc ggcaaggttc
     1381 acgggtgtgt cgagaatcgg caccgctcgc gggagttcat agcctttctc cagaagatcg
     1441 acgccgccta ttcgaccgac accgccatca agctgatcct cgacaatcac tcggcgcaca
     1501 tctccaagga gaccaagacg tggcttgccg cccagccaga cggccgcttc accttggtgt
     1561 tcacccc
//
LOCUS       DATAQL010001327         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_441100, whole genome shotgun sequence.
ACCESSION   DATAQL010001327 DATAQL010000000
VERSION     DATAQL010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441100"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..618
                     /locus_tag="VHS97_22850"
     CDS             367..618
                     /locus_tag="VHS97_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451112.1"
                     /translation="MGEVTRFALLAALDEDPDNLAEEFCRADRRRHLSPLSSELSVSH
                     EHPNNFRLIERGLVYGPLDGFRLYLLALVKELLYPSDHV"
     gene            1153..1746
                     /locus_tag="VHS97_22855"
     CDS             1153..1746
                     /locus_tag="VHS97_22855"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEX3451113.1"
                     /translation="MLAFLLEFLWSPPTALVLACTPIVTSDHFRERGVNKMFKQIWSA
                     IGILGLAAAMAIQATPAQAQGGVRVGTLNCNVASGWGFVFGSSRELRCVFSAGPGHED
                     HYTGSISRFGVDIGFTRGGVLVWAVFAPTTNLAPGALAGDFGGATAGAAVGVGAGANV
                     LIGGSNRTISLQPLSIEGNTGLNVAAGIGAMTLRHEP"
     gene            2051..>2612
                     /locus_tag="VHS97_22860"
     CDS             2051..>2612
                     /locus_tag="VHS97_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX3451114.1"
                     /translation="MSPIIITLLVLLGVGLAFALWLMGIYNRLVVAGNRFKNAFAQID
                     VQLKRRYELIPNLVEAVKGYMGHERETLDAVIKARNSAMTAEQKVAVNPSDPAAMKEF
                     NQAEAQLGGTLGRLFALSEAYPDLKANQNMLALQEELTSTENRIGFARQAFNDAATDY
                     NIRREVFPAVKVAGMFGFKEAALLETA"
BASE COUNT          479 a          770 c          793 g          570 t
ORIGIN      
        1 tttttgaggc aattcgcgat cgcgaattaa atttgcaaag ggtcttgccc gtgttgcgag
       61 tgcctcatca ctcaggtcag cccgcagcca acagttcggc tggtcagtgc agggcagtgt
      121 tccatggaac gggtcgctat agcggccgca ttccaatgaa ccgagtcatt gttccatgga
      181 acagagttgg gtttgggtgc gaacgaaatt cggttgccgc cgcgaacgtc agtttacgcg
      241 accctcgcgg tcgccattcg ctggtactcg atgcggagcc aggaggaaat ttcgcacctg
      301 ccggtcattc aggccgcccg ccctgagccg ctgggaaata gccgcgtcaa gttcctcgtc
      361 gcttaggtgg gcgaagtcac caggtttgcc ctgctcgctg cgctcgatga agaccccgac
      421 aatcttgccg aggagttctg tcgcgcggat cgccgccgcc acttgtcccc tctctcgagc
      481 gagctctcgg tcagccacga gcatccgaat aatttccgtc tgattgagcg aggtctcgtt
      541 tatggcccgc tggacggctt tcgcctctac ttgctcgcgc tggtcaagga gctcctctat
      601 ccgtctgatc acgtctgacc gttgtctgat tttccaggca gacttgcgat tgcccgataa
      661 gcctgcggct ttatgggcgt cttcaagctt ggcgcccttt atgatctctt gggcgaagca
      721 ttcatgacga acgtttcgga gcacagacat gatgctgtgt aatttaggcg atgatgccgc
      781 ttaaggccat cggtcgtgat gatgtcggtg cgcatgagca ggtcctccag atcggggcta
      841 gcacggcgct aaccatgacc acacacactc acactctctt ccgtcccata agccagtcgt
      901 ttgtcgataa tatcgtggct ttgttttgtt ggggtcagcc cgagcataac attgctcgtc
      961 gggccagcag cgccaaaaag ccgagcgttc atcgtttgcc gctcgttcct ttctccgggt
     1021 acgctgtgcc cggcggtgta cgatcatcgt accgcctggg accagcttgg gccggctaac
     1081 cgaagcgcag acggctgtcc tggcaaaaat tgggacgctc agcccggtat tgggattctg
     1141 gttcatctca atgtgctcgc attcctgtta gagtttttat ggagtccacc gacggctttg
     1201 gtcctcgctt gcacacctat cgtcacttcg gatcatttcc gcgaacgggg ggtgaataaa
     1261 atgttcaagc agatttggtc ggcgattggc atcttgggtt tggcggctgc gatggcaata
     1321 caggcgacgc cggcccaggc gcagggtgga gttcgagtcg gcacgcttaa ctgcaatgtc
     1381 gcgagtggtt ggggttttgt gttcggctcg tcgagagagc tgcgttgcgt cttctcagcc
     1441 ggacccggcc acgaggatca ctataccggc tccatttcca gattcggggt cgacatcggc
     1501 tttaccagag gcggggtcct ggtatgggca gtgtttgcgc cgaccacgaa tctggctcct
     1561 ggcgcgcttg ccggagattt cggtggtgcc accgcgggcg ccgcggtcgg cgtcggggcc
     1621 ggcgccaatg tgctgatcgg cggctccaac cgcaccatct cgctgcagcc gttaagtatc
     1681 gaaggcaata ccggtctcaa tgtcgccgcg gggatcggtg cgatgaccct tcgccacgaa
     1741 ccgtgatgtg cggctcactg acgccgatga ttttcgcgta tcgtgcttcc gttgcgggtg
     1801 cgtaccgagg gggccgttca gcggccgaag taagccgcat ttcggggaaa tatttgtcag
     1861 gctcgccggg accctccaac atacgtaatg ccaaggggtt gtgcgtagtc ggcccgccga
     1921 cgattgaaag gtcgacatag accgtccgtg gaatatgcca agttctcggc aggattccat
     1981 aagataagtc ttgcgccagc cgttgtgcga tggaccagac caacagtcct ctcgtcggag
     2041 gtcagggcca atgagtccca tcataatcac cctgctcgtg ctcctgggcg tcgggctggc
     2101 gttcgcgctc tggctcatgg gcatctacaa ccggctggtg gtcgccggga accgcttcaa
     2161 gaacgctttc gcccagatcg acgtgcagct caaacgccgg tacgagctga ttccgaacct
     2221 ggtcgaagcc gtcaagggtt acatgggcca cgagagggag acgctcgacg ccgtcatcaa
     2281 ggcccgcaac agcgcgatga cggccgagca gaaggtcgcg gtcaacccca gcgacccggc
     2341 ggccatgaag gagtttaacc aggccgaggc ccagctgggc ggcaccctgg gccggctctt
     2401 tgccctgtcc gaggcgtacc cggaccttaa ggccaaccag aacatgctcg ccctgcaaga
     2461 ggagctgacc tcgaccgaga acaggatcgg cttcgcccgg caagcgttca acgacgccgc
     2521 caccgactac aacatccggc gcgaggtctt cccggccgtc aaggtggccg gcatgttcgg
     2581 cttcaaggag gcggccctgc tcgaaaccgc ag
//
LOCUS       DATAQL010001328         4789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_441109, whole genome shotgun sequence.
ACCESSION   DATAQL010001328 DATAQL010000000
VERSION     DATAQL010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441109"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4789)
                     /locus_tag="VHS97_22865"
     CDS             complement(<1..>4789)
                     /locus_tag="VHS97_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451115.1"
                     /translation="VEEAVKGFNSANSLVTDALKAQFQALAGITLSNRIRVTGEAERS
                     WLITDPGRSVYLVEELTGPRRLAVSIASLELVATDLTAMITGPNADRFGYDLDARSFF
                     INPVLPDRRQPYREFTIIYHFAGPGAVQAFSQFRNANSSAALTAGNDQFAINYGSAAI
                     GAEILANRLGVGPMGNADAVDLKYEEFFLSSWAVGDPAMVVDVPANARNSAVDATDTG
                     TRVAQKPLFTIDVIDNSLTQAMGNGSGVVPQALKDAFAANGVALAAQAIVTTNIPQVL
                     STGANPAVKSTPGQWIVLDPFSRNNAPAADRDRYSIVSSALRNEGGKPHMTLTVYKGF
                     PASNVSALTFNSLAHEKDPKIKATQAYYPDDPSNVYHSYIRDHVKFRILHAGPGPSHV
                     HHLHAHQWLHAAGSSNSQYLDSQLIVPGSAYTLEIVHNGSGNRNLTVGDSIFHCHFYP
                     HFAQGMWSLWRSHDVLEAGTWLDAAGRPVTHVIRVNDRDYPVSREANDGKIVLFYQDD
                     QGTRREIADVNQVKRAWNRALPDGEIEAGTPIPALVPLPSLAMPLLPARVRLTDLDPF
                     LTTGSKGQGRRVEVEPTPGSGNGYDNPGYPFFIPGIGGHRPPHPPLDFAWREDANGNR
                     IPAISAQTVGGAPAWGTPPQRKTTELARGIVNPGVAAGTALAFFSSGTGGRAMMGSLL
                     VQAPTSPPEPPATISIDGTVVAGAPTWVVNNNPSAPAVSVVVKPGDNVVWKAGTDNVG
                     VAFATQAEAETVLHFLPGGSLDGGLPRHVVLGGQIVKEYSTRWDFTRDFIAYDKAKRP
                     IAGKLSALHLPEDGTPLEHAAMKHHSTRTHRSYLPDGKLGNITRNGLVAKPGAPYAPP
                     EVDEYGNADFNPRRYQAAVIQTDVVFNKLGWHFPQQRMLTLWEDVADMTGGRRAPQPL
                     FFRSSTNDTIEYWHTNLVPSYYELDDFQVRTPTDVIGQHIHNVKFDVTASDGAANGFN
                     YEDGTFSPDDVRDRISAINRPGGPFGQGGILAFNPRTGFVNSGATPEKLRVVPVHEAY
                     PKRANAGDEQNGLFGKPPLGQDWDGAQTTIQRWDADPLLNDRGMERTLRTIFTHDHLG
                     SSTHQQVGLYAGLVVEPENSLWYLPNGERMNRRADGGPTSWEGFIQTKDPQKSIREFV
                     LEFQDTQLVYNKQSMTVVSGARFDPTASASASASAAFDVGQYGELSGAVGKKNGGSAR
                     ADIPAFVRILNRDEIPEAFGNLIFPSMGIKLTSKATAHRAKMVPDNHEWVIEEPTDSD
                     LNAGAIYRLVANNDESSLLVYTPGIAPGWSDPKNALNPPGDGNNRTTGAPFPQLVSSG
                     SVGTYTMNYRNEPALARLTGGGGGQLDATDPAFVFSSIPRTDPALNTQPTPGSPINPP
                     PQPPAPTVIEIDGDIVSGTPTWVLKGGGSAANVTVKPGDVIVWKAVTGKHGVVFDTQA
                     VAQAFLDFQQGAGFPVLGPQTVGTETVWGTAPQAGQASGTMLAQATVKAGVAPGTTLG
                     FFCSQHGRAMSGSLATAAAAAAIEIDGSIVNGTPTWVLKGGGSAANVTVKPGDVIVWK
                     AVTGTHGVVFDTQAVAQAFLDFQQGVGIPVLGPQTVGTETVWGT"
BASE COUNT          815 a         1479 c         1508 g          987 t
ORIGIN      
        1 gtcccccaca cggtctcggt cccgaccgtc tgcgggccaa gcactggtat tcccacgcct
       61 tgctggaaat ccagaaacgc ttgagccacc gcctgcgtgt caaacaccac tccgtgtgtg
      121 cctgtcacgg ccttccatac aatgacatcg cccggcttca ctgtaacatt cgccgccgac
      181 ccgcctccct tcagaaccca tgtcggcgtg ccgttgacga tgctgccgtc tatctcaatc
      241 gctgccgctg ccgcggccgt tgccagtgac ccgctcatcg cgcggccatg ctgactacag
      301 aaaaacccca gcgtcgttcc aggcgccact cctgctttca ctgtcgcctg tgccagcatc
      361 gtcccagacg cctgccccgc ttgcggcgcc gtcccccaca cggtctctgt cccgaccgtc
      421 tgcggcccaa gcaccggaaa tcccgcgccc tgctggaagt ccagaaacgc ttgcgccact
      481 gcctgcgtgt caaacacgac tccgtgcttc cccgtcacgg ccttccatac aatgacatcg
      541 cccggcttca ctgtaacatt cgccgccgat cctcctccct tcagaaccca tgtcggcgtg
      601 ccgctgacga tgtcgccgtc aatctcaatc acggttggcg caggtggttg cggaggcggg
      661 ttgatcggtg agcccggtgt aggctgagtg ttcagagccg gatccgttcg cgggatcgaa
      721 ctgaatacga aggccgggtc agtcgcgtcc agctggcctc cgccaccccc cgtgagccgc
      781 gcgagcgctg gctcgttgcg gtagttcatc gtgtaagtcc cgacagaccc agagctgacg
      841 agctgtggga atggggcacc tgtcgtcctg ttattgccgt cgcccggcgg gttcagggca
      901 ttcttcgggt cagaccaccc tggcgcgatt ccgggggtgt agacgaggag agacgactcg
      961 tcattgttgg cgacaagacg gtagatcgcc ccggcgttga ggtcgctgtc tgttggttcc
     1021 tcgatcaccc attcatggtt gtccgggacc atcttggcgc gatgggccgt tgctttgctg
     1081 gttagcttga ttcccattga ggggaagatc agatttccga acgcctctgg gatttcatcg
     1141 cgattgagga ttctcacgaa cgctggaata tccgcccgcg cgctcccccc gttcttcttg
     1201 ccgacagcgc cggaaagctc gccgtactgt cccacgtcga atgcggcaga ggcagaggca
     1261 gaggcgcttg cggtcgggtc gaatctcgcg ccgctgacga ccgtcatgct ctgtttgttg
     1321 taaacgagct gtgtgtcctg aaactcgagc acgaattcgc gaatgctctt ctgcgggtcc
     1381 tttgtctgaa taaaaccctc ccagcttgtg ggcccgccgt cggcgcgacg attcatccgc
     1441 tcgccattgg gcaagtacca caacgagttt tcgggttcga cgacgagccc tgcatacaag
     1501 ccgacctgtt gatgagtcga ggacccgagg tggtcgtgag tgaagatggt acggagagtg
     1561 cgctccattc cgcgatcatt gagcaagggg tcagcatccc agcgctggat cgtcgtctgg
     1621 gcaccgtccc aatcctgacc aagtggcggt ttgccgaaaa ggccattctg ctcgtcccca
     1681 gcatttgcac gcttcgggta ggcttcgtgc accggtacaa cgcgcagttt ctccggagtg
     1741 gccccgctat taacgaagcc ggtgcgcgga ttgaaggcca ggattccgcc ttgcccaaat
     1801 ggtccgccgg ggcggtttat cgcactgatc cggtcgcgaa cgtcatcggg gctgaaggtg
     1861 ccgtcctcgt agttgaagcc attggcggca ccatcggaag cagtgacgtc gaacttcacg
     1921 ttgtggatgt gctgaccgat cacgtcggtt ggggtccgca cctggaaatc gtcaagttcg
     1981 tagtaggacg gaaccaggtt ggtgtgccag tattcgatgg tgtcgttggt gctcgagcgg
     2041 aaaaacaggg gctgaggggc ccggcgcccg ccggtcatgt cagcgacgtc ctcccaaagt
     2101 gtcagcatcc gttgctgcgg gaagtgccaa cccagtttgt tgaagaccac gtcggtctgg
     2161 atgacggccg cttgataccg cctggggttg aagtcggcat tgccgtattc atcaacctca
     2221 ggtggcgcgt acggagctcc cggctttgcc accagtccat tacgggtgat gttgccgagc
     2281 ttgccgtcgg gcaggtacga gcggtgggtg cgcgtcgagt ggtgtttcat ggcggcgtgt
     2341 tcaaggggcg ttccgtcttc aggcaggtgc aatgcgctga gcttgccggc gatcggccgc
     2401 ttggctttgt cgtatgcgat gaaatcgcgc gtgaagtccc agcgagtcga gtattccttg
     2461 acgatttgac cgccgaggac gacgtgccgc ggcaggccgc cgtcgagcga gccgccgggc
     2521 aggaaatgga gcaccgtctc ggcctcggcc tgcgtggcaa aggcgacgcc gacgttgtca
     2581 gtgccagcct tccagacgac gttgtccccc ggtttgacga cgacgctcac ggccggcgcg
     2641 gacggattgt tgttcacgac ccaggttggc gctcccgcga cgaccgttcc gtcaatcgag
     2701 atggtcgccg ggggctctgg cggcgacgtc ggggcctgaa ccagtaacga gcccatcatc
     2761 gcgcgaccgc ctgtgccgct tgagaaaaag gccagcgccg tgcctgcggc aacccccggg
     2821 ttgacaatcc ctcgcgccag ctcggtcgtc ttccgttgcg ggggcgtgcc ccaagccggc
     2881 gccccgccaa cggtctgtgc gctgatggcc ggaatccggt tgccgttggc atcctcgcgc
     2941 catgcgaagt ccaagggcgg atggggcggc cggtggccgc cgattccggg aatgaaaaag
     3001 ggatagcctg ggttgtcgta cccgttccca ctacctggcg tgggctcgac ctccacgcgc
     3061 cgcccctgcc cctttgagcc ggtcgtcaga aatgggtcca ggtcggtgag tctcacgcgc
     3121 gccggcaata gcggcattgc aaggctcggc agcggcacca gggccggaat cggcgtacca
     3181 gcttcaatct caccgtcggg tagagcgcgg ttccacgccc gttttacttg attgacgtcg
     3241 gcgatttcac gccgggtccc ctggtcgtct tggtaaaaga gcacgatctt gccgtcgttg
     3301 gcttcccgag aaaccggata gtcccggtcg ttcaccctga ttacgtgcgt gaccgggcgg
     3361 ccggcggcgt caagccatgt gcccgcctca agaacgtcgt gcgaacgcca gagcgaccac
     3421 attccctggg caaagtgcgg gtaaaagtgg cagtggaaga tcgagtctcc caccgtgaga
     3481 ttgcggttgc cgctaccgtt gtgaacgatc tcgagcgtgt atgcagagcc cgggacaatg
     3541 agctggctgt ccaggtactg gctgttcgag cttcctgcgg catgcagcca ctgatgggca
     3601 tgaaggtggt ggacgtgcga cggaccgggg ccggcatgaa gaattcggaa cttcacgtga
     3661 tcgcgaatgt aactgtgata tacgtttgac gggtcgtccg ggtagtacgc ttgggttgcc
     3721 ttgatcttcg ggtccttttc gtgggccagg ctgttgaatg tcaaggcgct tacgttgctt
     3781 gccgggaagc ccttgtagac ggtgagcgtc atgtggggtt tacccccctc atttcgcagg
     3841 gcagagctga cgatcgaata gcgatcgcga tcggcggccg gcgcattgtt ccggctgaaa
     3901 gggtcgagca cgatccactg accgggcgtg ctcttcaccg ccgggttggc accggtagag
     3961 agcacttgcg gtatgttcgt tgtcacgatt gcctgggcag cgagtgcgac tccgttggcc
     4021 gcgaaggcgt ccttgagcgc ctgtggaacg accccgctgc cgttgcccat cgcctgggtc
     4081 aggctgttgt cgatcacgtc gatggtgaac aggggctttt gtgccacccg cgttcctgta
     4141 tccgtggcgt cgaccgcaga gttgcgggca ttggccggca cgtcgacgac catcgccggg
     4201 tctcccaccg cccatgagct taagaaaaac tcttcatact tcaagtcgac ggcatcggca
     4261 ttgcccatcg gcccaacacc cagccggttg gcgaggatct ccgcgccgat ggcggccgac
     4321 ccgtagttga tcgcgaactg gtcgttgccc gcggtaagcg ccgcgcttga gttggcgttc
     4381 ctgaactgcg aaaacgcctg cacggcccct ggtcccgcga aatgatagat gatcgtgaac
     4441 tcgcgatacg gctgccgccg gtcggggagc acggggttga tgaaaaacga acgtgcatcc
     4501 aggtcgtaac cgaagcggtc ggcgttcgga ccggtaatca tcgccgtcag gtctgtcgcc
     4561 accagttcaa ggctcgcgat cgagaccgca agccggcggg ggcccgtcag ctcctcgaca
     4621 agatagaccg atcggcccgg gtcggtgatc agccacgatc gttccgcctc tcccgtgacc
     4681 cggatgcgat ttgaaagcgt gatcccggcc agcgcctgaa actgcgcttt gagcgcgtcg
     4741 gtcaccaggc tgttggcact attgaatccc ttgacggctt cttccacgt
//
LOCUS       DATAQL010001329         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3343937, whole genome shotgun sequence.
ACCESSION   DATAQL010001329 DATAQL010000000
VERSION     DATAQL010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3343937"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VHS97_22870"
     CDS             complement(<1..320)
                     /locus_tag="VHS97_22870"
                     /inference="COORDINATES: protein motif:HMM:NF027503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3451116.1"
                     /translation="MSDSYVWRPTQAVMERSNIGRFMRRNGIAGYHDLVARSTSDIEW
                     FWNAVIEDLGIEFYRPYERLLDAGGGMPWSRWFVGGQINLVHNCLDRHIRSSRRNLTA
                     VIWEG"
     gene            complement(632..1240)
                     /locus_tag="VHS97_22875"
     CDS             complement(632..1240)
                     /locus_tag="VHS97_22875"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3451117.1"
                     /translation="MTGLLTNAQRSYHFDVRQFHRMMAAGIFDDQKVELVAGKIYAMT
                     DLPPHIFAVGRLHEGLRSVLLRDEWTIREEKPVLFGRYWAPKPDIAVLRGSDAIYAAR
                     HPRPRDVVLLVEVSDTTYHRDRGRKWRRYAAACIPIYMIVRLKGPETLIEVWTGPTGR
                     GRVARYTDVVRYSALAGESVPIEVDGSVHGQVAVVDLVTRQA"
     gene            1531..2634
                     /locus_tag="VHS97_22880"
     CDS             1531..2634
                     /locus_tag="VHS97_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451118.1"
                     /translation="MRRSLCKGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRMTC
                     VNNLKQIGLALHNYHEQQGTFPMGSGQPTMDKVGGQVPIAKQGLSIHVAILPQLGEMP
                     VYNAINFSFGMADNTPLYAFQVNQTAQKTQINEFLCPSDPNATTSLDTSTATGNNNYY
                     GCIGATTATFGPVLGDPVTGSAGMNWSSIPYTGLFAYQQSKGVQNVTDGTSNSIAFAE
                     GTVGAATETPRQKLIGLTGVSIPAAALQFNVFTNPVGVQSGIAACTAAWSAGTSPDVQ
                     RGDSWVHGSMAMTLFNTVVVPNGESDQWTYCSDTGSGSASRFSNSDSYHSGGVNVLMA
                     DGHAQFIKNSISQPTWWSLGTVAGGEVISSDSY"
     gene            2882..>3187
                     /locus_tag="VHS97_22885"
     CDS             2882..>3187
                     /locus_tag="VHS97_22885"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX3451119.1"
                     /translation="MKSYVALAGTTPLMAGLLKAQSAEAAGPRLAYVGTFSSPLRDVL
                     PTQVDLPPGNGRGIHVFQVNRSTGAMTPAGIHEMGTSPSCLALNSAGTRLYSANETDR
                     "
BASE COUNT          644 a          995 c          917 g          631 t
ORIGIN      
        1 ccctcccaga tgaccgctgt caggttgcgc cgtgaggatc ggatatgacg gtcgaggcaa
       61 ttatggacga ggttgatctg tccaccgacg aaccaccgcg accagggcat gccgccgccg
      121 gcgtcgagaa gccgttcgta cgggcggtaa aactcgatac cgagatcctc gatcaccgcg
      181 ttccagaacc attcaatgtc cgatgtcgat cgggcgacga ggtcgtggta gccggcgatc
      241 ccattccggc gcatgaagcg gccgatgttg gaacgttcca tgacagcctg agtcggcctc
      301 cagacataac tgtcagacaa ggaacctccg atcagagtcc ttaagacgtt gaatcgcacg
      361 aaagcccgac ctatcccacc ctccggcgcg cgtttgaacg gccgtgacgg ttgtgaggtt
      421 gagatgcagg ccaaagtcta tcgggaagtc cgccaagctc tcgttcgccg gttccttcat
      481 tggatttatg atcgctagag ggcgtatcga gtcgtagtgc tggcggtgta tggtgagccc
      541 cgggtacgtt tccgctcggc cacaaattga agagggagca gccactcacg ttcatctgtg
      601 atccgctggc gcactggcta ctggagagtg tctaagcctg ccgagtgaca agatcgacta
      661 ccgcaacctg accgtgtacg ctgccgtcca cttcgatggg gaccgactcg ccggccagtg
      721 cgctgtagcg aaccacatcg gtgtagcggg ccaccctgcc tcgcccggtt gggcccgtcc
      781 agacctcgat gagcgtttca ggtcctttga gacgaactat catgtagatg ggaatgcatg
      841 ccgcggcata tctgcgccac tttcgcccgc gatcgcggtg gtaggtcgtg tcggagacct
      901 caaccagaag aacgacgtcg cgaggtcgcg ggtgccgcgc ggcgtagatg gcatcgcttc
      961 cgcgcagcac cgcaatgtcc ggcttgggcg cccagtagcg accgaacaag accggctttt
     1021 cctcacggat ggtccactcg tctcgcagaa gcacggaccg cagcccctcg tgaagccgcc
     1081 cgacggcgaa gatgtgcggt ggcaggtcgg tcattgcata gattttcccg gcgaccagct
     1141 cgaccttctg gtcgtcaaag atgccggcgg ccatcatgcg gtgaaattga cggacgtcaa
     1201 agtgatagct tcgttgagcg tttgtcaaga ggccagtcat tgggcaaatc ctccttgctc
     1261 aaggtagcgg aattcgacat ctgaagcaag cgagccatcc gaggcacggc actcccgaat
     1321 tcgcgcgcgg gtttacattt gaaccgctcc aaccagcgtc gcgagactca cccgctccga
     1381 gccagattca tgaccgcgga cctcaaatga ctgagacatg tcgttattct tttcgccttt
     1441 gattgtgtta gcagatattc ttgtgaggag attcatcgca gccacggagc tccattgcgg
     1501 accccaagcc cacacttatg gagtactccc atgagacgat ccctttgcaa aggctttacc
     1561 ctgatcgaac tgttggtggt gatcgccatc atcgccgtct tgatcgcgct gctgcttccc
     1621 gccgtgcagg cggcccgcga ggcggcccgc cggatgacgt gcgtcaacaa cctcaagcag
     1681 atcgggctcg ccctgcacaa ctaccacgag cagcagggca ctttcccgat ggggagcggc
     1741 cagcccacga tggataaggt gggcggccaa gttccaatcg ccaagcaagg cttgagcatt
     1801 cacgtcgcga ttttaccgca actcggagaa atgccggtct acaacgcgat caattttagc
     1861 tttggcatgg cggacaacac gcccttgtac gccttccagg tcaaccaaac ggcgcagaaa
     1921 acgcaaatca acgagttcct ctgcccctcc gatccgaacg cgaccacttc gcttgatacc
     1981 tccacggcga ccggcaacaa caactactac ggctgcatcg gggccacgac cgcgactttt
     2041 ggacccgtcc tgggcgatcc ggtgaccggt agcgcaggga tgaactggtc ttcaatcccc
     2101 tatacgggac tcttcgctta ccagcaatcc aaaggggtcc agaatgtcac cgatggcacg
     2161 tccaacagca tcgccttcgc ggaagggacc gtcggcgcag cgacggagac gccccggcag
     2221 aagttgatcg gactgaccgg ggtgagcatt cccgccgcag ccttgcagtt caacgtcttc
     2281 accaatccgg tcggtgttca gtccgggatc gcggcctgta cggcggcctg gagtgctggc
     2341 acgagccctg acgtccagcg cggtgattcc tgggtgcacg ggtcgatggc catgaccctg
     2401 ttcaataccg tggtcgttcc caacggcgag tccgatcagt ggacttattg cagcgatacc
     2461 ggctccggct cggcatcacg gttcagtaac tccgacagct accactccgg cggcgtcaac
     2521 gtacttatgg ccgacggcca cgcccagttc atcaagaatt cgatcagcca gccaacttgg
     2581 tggagcctcg gaacggtcgc cgggggcgag gtgatcagca gcgactccta ttgatggccg
     2641 ttgattgatt gatcacagcc cacgctccat ccgccgatcg cgtgtgtgct gacccgaaca
     2701 atcgccctcc gggatctcct gggaaagata gagtagtggc gtcacagacg tccgccgcgc
     2761 tctggttcat ttttgccgga attgctgtta cgcacctgat catgctcctc gcaccaaccg
     2821 tcccctcaac acgcaaggcc gtttgcacat gaagccgctc gctagttccc gacgctcgtt
     2881 tttgaaatcc tatgtcgcac ttgcggggac aacaccgctc atggcgggtt tgctcaaagc
     2941 gcaatcggca gaagccgccg gcccacggct cgcgtatgtc ggaaccttca gttctccgct
     3001 ccgcgatgtg ttgcctaccc aagtcgatct gccgcccggc aatggtcgcg gcatccacgt
     3061 gtttcaggtg aatcgctcca ccggggcgat gacgcccgcc ggcatccacg agatgggcac
     3121 gagcccgagc tgcctggctc tcaatagcgc cggaacccgt ctgtattcgg ccaacgaaac
     3181 ggatcgg
//
LOCUS       DATAQL010001330         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_136631, whole genome shotgun sequence.
ACCESSION   DATAQL010001330 DATAQL010000000
VERSION     DATAQL010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136631"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VHS97_22890"
     CDS             <1..891
                     /locus_tag="VHS97_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3451120.1"
                     /translation="TPLAPRREALEALLQAKDPSLASTLHGLVRDSGLGGHAVRGLSA
                     YDDPATPDVLIKAYKSLGAPERRDALNTLAARKPWAKELLAAVEAGKLPRTDLTADLV
                     RQLRNLKDPSLDAQLVQVWGNVRETTGDRARLIAQYKSLLSSKPARAPEPLLGRAVFA
                     KVCQQCHTLFGVGGQVGPDLTGSNRADLDYVLSNVLDPGALIGKDYLAHVIATTDGRV
                     LTGIIRAEDKDKITLVTANETITLPKSEIEERRSSEQSMMAEDLWKPLSEHEIRSLVS
                     YLASPAQVALPAGETGRSAP"
     gene            complement(1056..2111)
                     /locus_tag="VHS97_22895"
     CDS             complement(1056..2111)
                     /locus_tag="VHS97_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451121.1"
                     /translation="MPQNRRRPYSRAFTLIELLVVIAIIAVLIALLLPAVQAAREAAR
                     RMQCTNNLKQIGLALHNYHDSVNSFPPGYVSAIDRTVLDACNMDAESQHGVDLGSGWA
                     WGSMILPFLEQQPLYNSINFNLSVAFRDNDTCSLNPLAVYLCPSDPGPSVVPVFEDPP
                     DPNNPGSFNATHIVDTLSRGNYVGMYGIGEVCAQSGALDSPNNNGAGPQGQHAGIFYR
                     NSPTSVAAVTDGTSNTIMVGERSHNLSYVTWVARSIDGWLGKTSPIEGGTDQFNPSPE
                     ECWTQIMGPCGLEDGLRTINNPEAHVEDYWSMHPGGANMLFGDGSVHFLKSTINPIPW
                     RAMATRAFGEIVSSDSY"
     gene            complement(2143..>2713)
                     /locus_tag="VHS97_22900"
     CDS             complement(2143..>2713)
                     /locus_tag="VHS97_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3451122.1"
                     /translation="MPRAAVEQPTQQIDFAAATRPLLGSLVAAARRILRDEDAAWDAV
                     QEALISLWRESEMPLNVRSWLLRTVAHRSLHLARCRSRRLEHEREAGLARGEESDRDD
                     PARRLEGEELGCIMEKALCRIAPDHHAVLVKSVIEQMDYESIAMSLQIPIGTVRSRMN
                     RARRSLREVLIHNLPDEYLIRPHSRPEST"
BASE COUNT          521 a          800 c          866 g          526 t
ORIGIN      
        1 acgccccttg ccccgcgacg cgaggcactc gaggcattgc tccaggcgaa agacccatca
       61 ctggcgtcga ccctccacgg tttggttcgc gactccggac tgggggggca cgctgtccgg
      121 gggctttcgg cctacgacga cccggcgact ccggatgtct tgatcaaggc gtacaagtca
      181 ctgggagctc ccgagcgccg cgatgccctg aacactctcg cggcccgcaa gccatgggcc
      241 aaagaattgc tggcagcggt cgaagcgggc aagctgccgc gtacggactt gaccgccgac
      301 ctggtacgcc agctccgcaa tttgaaagac ccaagcctcg atgcacagct cgtccaggtt
      361 tggggtaacg tccgcgagac gacgggcgac cgtgctcggc tgatcgcgca gtacaagtcg
      421 ctcctcagct caaaaccggc gcgggctccc gagccgttgc tgggtcgggc tgtcttcgcc
      481 aaggtatgcc agcagtgcca cacgcttttt ggggtgggtg gtcaggtcgg gccggacctg
      541 acgggctcga accgggcgga cctcgattac gtgctttcca atgtcctcga tcccggtgcc
      601 ttgatcggta aggactatct ggctcacgtg atcgccacca ccgacggccg cgtgttgaca
      661 ggcatcatcc gcgccgagga taaagacaaa atcactctcg tgacggccaa cgagacaatc
      721 acgctgccga agagcgagat cgaagagcgg cggtcaagtg agcaatcgat gatggccgag
      781 gacctctgga agccgctctc cgagcatgaa atccgctcct tggtttctta cctcgccagc
      841 ccggcgcagg tcgcgctgcc tgctggcgag accgggcgga gtgctccttg acgatcagat
      901 cccgcgagcg ctctgtttaa agacagaccc ctccgtcgat cgagcgggcg catgactatg
      961 aatgtacgac gaccggggct ggaccgaccg gtggagagcg gtcggaacgt gccccggtcg
     1021 tcgtgggggg ggttcggctg atgaacgact tgtggtcaat acgaatcgga agagacgatc
     1081 tcaccgaagg ctcgggtagc catcgcacgc cagggaatcg gattgatcgt cgacttgagg
     1141 aaatgcaccg aaccgtcccc aaacagcatg tttgccccgc ctgggtgcat gctccagtag
     1201 tcctccacgt gagcttcggg gttgttgatc gtgcgaaggc catcctccag cccacagggg
     1261 cccatgatct gcgtccagca ctcctcgggc gatgggttga actggtcggt gcctccttcg
     1321 atcggtgacg tcttgcccag ccacccgtcg atcgatcggg ccacccaggt gacgtagctc
     1381 aggttgtggc tccgctcacc gaccatgatc gtgttgctcg tcccatcggt gacggccgcg
     1441 acggatgtcg ggctattgcg gtagaaaatt ccggcatgct ggccctgagg gccggcgccg
     1501 ttgttatttg gcgagtcgag agcacccgac tgcgcgcaga cctcgccgat gccgtacatg
     1561 cctacataat tgccgcgcga aagggtatcg acgatgtgcg tggcgttaaa actgccgggg
     1621 ttgttcgggt cgggcgggtc ttcgaagacg ggtacgacag atggaccagg gtctgagggg
     1681 cagaggtaga cggcgagcgg gttcagactg catgtgtcgt tgtcgcggaa ggccaccgag
     1741 aggttgaagt tgattgagtt gtataacggc tgctgttcca ggaagggcag gatcatgctt
     1801 ccccatgccc aacccgagcc gaggtcgacg ccgtgctggc tttccgcgtc catgttgcag
     1861 gcatcgagaa ccgtccgatc gatcgcgctg acgtagccgg gcgggaagga gttcacactg
     1921 tcgtgatagt tatgcagtgc caggccgatc tgtttcaggt tattagtgca ctgcatgcgt
     1981 cgggcagcct cgcgggccgc ctggacggct ggcaaaagca gcgcaatcaa cacggcaata
     2041 atcgcgatga cgacgagcag ctcaatgagt gtgaaagccc ggctgtaagg tcttcttcga
     2101 ttctgaggca tacgttgtct cccgatttca ataaatgaag tttcaagtgg attcagggcg
     2161 gctatgagga cgaataaggt actcgtcggg caagttgtga atgagcacct ctcgaagcga
     2221 tctacgggct cgattcattc tcgatcgcac cgtaccgatg gggatttgta acgacatggc
     2281 gatcgactcg taatccatct gttcgatcac actcttgacc agaacagcgt ggtggtccgg
     2341 agcgatcctg cagagcgctt tttccatgat gcacccgagc tcttcacctt ccagacgacg
     2401 agccggatca tcgcgatcgc tctcttcgcc acgcgccaat ccggcttccc gttcgtgttc
     2461 gagacgacgg gagcggcagc gtgcgagatg gaggctccga tgagccacag tgcggagtag
     2521 ccaggagcgc acgttgagcg gcatctcgga ttcgcgccac aagctgatca aggcctcctg
     2581 gactgcatcc caggcagcat cctcgtcgcg gaggatccgc cgcgcagcgg cgacgagaga
     2641 tccgagcagc gggcgcgtcg ctgccgcgaa gtcgatctgt tgggtcggtt gttccaccgc
     2701 cgcgcgtggc ata
//
LOCUS       DATAQL010001331         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1817108, whole genome shotgun sequence.
ACCESSION   DATAQL010001331 DATAQL010000000
VERSION     DATAQL010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817108"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VHS97_22905"
     CDS             complement(<1..896)
                     /locus_tag="VHS97_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX3451123.1"
                     /translation="MENEWVRPTLLVVDDERDVLSSVHDLLRIDYRVITYERGQEALE
                     YLRTGQAADAILCDQRMPEMTGVDVLRQVKAIRQETTRLLFTAYSDIRTVIDAINQGH
                     VFRYLGKPCDPDELAAVVRQAVEHHNLIVEKNALLAELRESNERLTEANRLKGAFIEV
                     ASHELNTPVTVVLGMLELWKMSQSPSASPQERQWVDRITGAAARLARTVERMLKLVRN
                     REFSQSLDKQPTELEPLVQQAIDELSPYLELRSQAVSVSVEPNLGSLDLDQSKIADVL
                     TNLLANAVKFTPDGGTIRVEART"
     gene            complement(899..1795)
                     /locus_tag="VHS97_22910"
     CDS             complement(899..1795)
                     /locus_tag="VHS97_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3451124.1"
                     /translation="METALIVEDHPEQAELVARILRLRDYEPILAEDGETGLRLARQH
                     LPDVMLLDLMLPDINGFDVCRRLRTDRATMMIPVVMLTALNDVQHQVHGFRVGANAYV
                     TKPYGVDDLFEAITAARDWRTSIEKRALRGEIHVELNSEINFLKDLNDFLMLVCQATP
                     LSNDQVIQLRQAVMEMAHNAIEWGNLHQSDRLVNITYLIYDDRLEIEVRDQGPGFDRT
                     SLPHAAVAGDPFTHLDVREKLGLRAGGFGLLICQGMVDEMRYNDIGNEVTLIKRFASR
                     RRPLDGSSPPSAIDSDDENETR"
     gene            complement(1883..2878)
                     /locus_tag="VHS97_22915"
     CDS             complement(1883..2878)
                     /locus_tag="VHS97_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX3451125.1"
                     /translation="MPANLPPQYLKADQEYRQATTPLERLEKLRELFRLLPKHKGTEK
                     LQSDLKQKISQLKDEQERGIRGGKKTGLSYHVPREGAGQVMLIGPPNTGKSTLLAALS
                     NARPEIAPYPFTTRVPQPGIMMWQDVPVQLVDMPPVSAEFVESWVPNVIRSADAALLV
                     ADLASDDVADATIEVLERLASTHTELVGELPYDIEDESIRHLKTMLVASKLDADGARE
                     RLDVLSEWFAPKFPILAVSVHSKEGLETLPIATYHLLGVVRIYTKVPGKPADRSKPFT
                     LPIGSTVLDLAREIHRDLEHTMKSARIWGSGVFDGQSVKRDHELHDGDLIELHTS"
     gene            3163..3684
                     /locus_tag="VHS97_22920"
     CDS             3163..3684
                     /locus_tag="VHS97_22920"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX3451126.1"
                     /translation="MYVLDFLRSHGVWFETLLHRPVSSSAKRAGSVHVPGRRVAKAVL
                     IKAGDSFLLVVLPSTSQIDLMRLSEVVGAPPALVRLGTPDELIELFPDCEPGVVPPFG
                     RLYGLTTLVDSGLADSAEIIVGANTRHEGLRMLFHDFQRLEEPIRASFTRPLGPEPQP
                     PNLSRAGRNRRAG"
BASE COUNT          724 a         1134 c         1078 g          855 t
ORIGIN      
        1 gtgcgggcct cgactcgtat cgtgccgccg tccggtgtga atttgacggc gttggccaaa
       61 aggttggtca gcacgtcggc aatcttggat tggtctaaat cgagtgagcc aaggttgggc
      121 tcgacggaga ctgacaccgc ctggctgcgg agctcgagat aaggcgacag ctcgtcgatt
      181 gcctgctgca ccagcggctc gagctcggtc ggttgcttgt cgagcgactg gctgaactcc
      241 cgatttcgaa caagcttgag catccgctcc acagtccggg ccagccgagc ggcggcgccg
      301 gtaatccgat cgacccactg gcgctcctgc ggcgaagcac tcggactctg cgacattttc
      361 cagagctcga gcatgccgag tacgaccgtc accggcgtgt taagctcgtg gctggcgacc
      421 tcgatgaacg cacccttcag ccggttcgcc tcggttagcc tctcgttgct ttcccgcagt
      481 tcggccagta gtgcgttctt ttccacgatc aggttgtggt gctcgaccgc ctgccgcacc
      541 acggcagcga gttcgtcggg gtcgcacggc ttgccgaggt agcgaaaaac gtgtccttga
      601 ttgatcgcat caatgacagt ccggatgtcg gagtaagccg tgaacaacag gcgtgtggtc
      661 tcctgccgga tggccttgac ctggcgcaac acgtcaacgc ccgtcatctc cggcatccgc
      721 tgatcgcaga ggatcgcgtc ggcggcctgg cccgtccgaa ggtactcgag cgcctcctga
      781 cctcgctcgt aagtgatcac ccggtaatcg atgcgcagta agtcatggac cgagctcaag
      841 acgtcgcgct catcatcgac gaccaagagc gtcggtcgca cccactcgtt ttccaacttt
      901 acctcgtttc attctcatcg tctgaatcga tggcggacgg cgggctggaa ccgtccagcg
      961 gccgacggcg cgaagcaaaa cgcttgatga gcgtcacttc gttgccgata tcgttatatc
     1021 gcatctcatc gaccatccct tgacatataa ggaggccaaa gcctccggca cgcaggccca
     1081 gcttttcgcg aacatcgaga tgcgtaaacg gatctccagc aacagccgcg tggggcaacg
     1141 acgtgcggtc aaagcctggc ccctggtcgc gaacctcgat ctccagacgg tcatcgtaga
     1201 taagataagt gatgttgact aaccggtctg attggtggag gtttccccat tcgatcgcgt
     1261 tatgggccat ttccatgacc gcctggcgca attgtatgac ctgatcgttc gacaggggcg
     1321 tggcctggca aaccagcatc agaaagtcgt tcaaatcttt gagaaaattg atctcgctgt
     1381 tcagctcgac atgaatttcc ccccgcaggg cgcgcttctc gatactggta cgccagtcgc
     1441 gggccgcggt gatcgcctcg aacaggtcat ccacgccata tggcttggta acgtaagcgt
     1501 ttgcacccac acggaaaccg tgaacctgat gctggacgtc gttcaaggca gtaagcatga
     1561 ccaccggaat catcattgtg gcgcggtcgg tgcgcaggcg ccggcagaca tcgaacccgt
     1621 tgatgtcggg caacatcaaa tccaggagca tcacgtcggg aaggtgctgt cgcgcgagac
     1681 gcaatccggt ctcgccatcc tctgcgagga ttggctcata gtcacgcagg cggagtatcc
     1741 tggcaaccag ttccgcctgc tcaggatgat cctccacgat cagagccgtt tccatcgtcc
     1801 gattccccgc actccttcgg gctgaagtcg caccacaagt tcacttcaat caagcagtta
     1861 ggcactacgt attggcgatc tttcacgagg tgtgcaattc aattaagtca ccgtcgtgca
     1921 gttcgtgatc gcgcttgaca ctctggccat cgaaaacacc cgaaccccaa attcgggcgg
     1981 atttcatcgt atgctcgaga tctctatgga tttccctggc aaggtccagc accgtgctgc
     2041 cgatgggaag tgtgaatggt ttggaccgat cggccggctt gccgggcacc ttggtataaa
     2101 ttcgcacaac acccagtaaa tggtatgtcg ctatgggtag cgtttccaga ccctccttgc
     2161 tgtgtaccga taccgcgagg atcggaaact tgggcgcaaa ccattcgctg agaacatcca
     2221 ggcgttcgcg cgctccgtcg gcatctagct tggatgctac gagcatcgtc ttaagatggc
     2281 gtatcgactc gtcctcgatg tcgtagggaa gctcgcccac cagctcggta tgagtggagg
     2341 ctagtcgttc caagacttcg atagtggcgt cggccacgtc gtcgctcgcc aggtcggcca
     2401 ccagtaaagc ggcatcggcc gaacgaatga cattcggcac ccacgactcg acgaattcag
     2461 ccgagacagg tggcatgtcg acgagttgca caggtacgtc ctgccacatc atgattcctg
     2521 gttgcggcac gcgggtcgtg aacgggtaag gggcgatttc aggacgtgca ttggaaagcg
     2581 ccgctaacaa cgtgctcttc cccgtgttcg gtggtccgat cagcatcacc tggccggccc
     2641 cctcacgtgg cacgtggtag gaaagcccgg tcttcttgcc ccctcttatt ccccgctctt
     2701 gctcatcctt gagctggctt atcttctgct tcagatccga ctggagcttt tccgttccct
     2761 tgtgtttggg gagcaaacga aacaattccc gtagtttttc cagccgctcc aggggggtgg
     2821 ttgcctggcg atactcctga tcggccttca gatattgcgg gggtaggttg gccggcatcg
     2881 tggtgtacct ccagggcgca ccaaacggac cctgggcacc gtatcgactt cggcgccgcg
     2941 atgcaagggc ctggtctcaa accgccgacg gcgcgagttg ctttatagca acactttgtg
     3001 aactgttgtc cgtcattccc agccttcagg tcgatcttac tgccgagttt gatgatttta
     3061 ataaatcaga cagcaccgaa tttccctcat tatccgatca tgtttcccag acactctata
     3121 cttcaagcag aagacatttc ttgcttttgg agttaacacc cgatgtatgt gctcgatttt
     3181 ctccgcagcc atggtgtttg gttcgagacg ctcttgcacc ggccagtatc atcgtcggcc
     3241 aagcgggcgg gaagcgttca cgtgccgggt cgtcgtgtcg cgaaagctgt gctgatcaag
     3301 gcgggtgact ccttcttgct agtggttttg ccatcgacat cgcagatcga ccttatgcgg
     3361 ctgagcgagg ttgtgggggc gccgcccgct ctggtgcgcc ttggcactcc ggatgagttg
     3421 atcgagctct tccctgactg cgagccgggc gtagtgccgc cgtttgggcg actgtatggg
     3481 ctgacgacgc tcgtggattc gggattggcc gattccgccg agatcatcgt cggggccaat
     3541 acccgccacg aaggacttcg catgctcttc catgacttcc agagacttga ggagccgatc
     3601 cgagcatctt tcacccgccc gctcgggccc gagccgcagc cgccgaattt atcccgtgcc
     3661 ggccgaaatc gccgtgccgg ttgaaaggac cagtcaagcc cgtccgcgcg cccttcgcct
     3721 tcgtccacgc gcccttcgcc ttgtcaccca cctttgtatg accgacggtt gtgtgcttgt
     3781 cgatctcaag t
//
LOCUS       DATAQL010001332         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1968510, whole genome shotgun sequence.
ACCESSION   DATAQL010001332 DATAQL010000000
VERSION     DATAQL010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968510"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VHS97_22925"
     CDS             complement(<1..221)
                     /locus_tag="VHS97_22925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX3451127.1"
                     /translation="MHFIQVGMLGALAALAIPIIIHLLFRQRARTVDLGTLQFLKIVL
                     RDNARRRRLKRWLLLALRMACVALIAFLFA"
     gene            complement(295..1119)
                     /locus_tag="VHS97_22930"
     CDS             complement(295..1119)
                     /locus_tag="VHS97_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451128.1"
                     /translation="MRLLISPILILSFAVAGDGPQGADSQAERLRAMRELADGVKMQI
                     VSSAPPQKLERLAEPIYRFDDPARRFFDGTVWAWGRSGRPAALLTLAKQRATTGGYLW
                     LAELTSLAPGQISATVPEIGAWQPASAGVVMRDLPKAPTPGEDATTRLRQMRELVRQV
                     KAYEFFAPQDQPRPERYELRVLPQPVHRYADANSGLIDGGLFMISYGLNPELVLLVEA
                     RRAKSSPPAWSFGVARISIAEIHVDFDGKEIWSHQGGYSKGPQDIYWLFTKSIAGE"
     gene            complement(1137..2033)
                     /locus_tag="VHS97_22935"
     CDS             complement(1137..2033)
                     /locus_tag="VHS97_22935"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3451129.1"
                     /translation="MPFDPRSLIDPAFFSRLESIELRARSIVEGSMHGLHRSPYLGLS
                     VEFATHREYTPGDDLRHLNWKLYARHDRLYIKEYDADTNLNLYLLLDASASMACANTG
                     RSKLHYAASLAAALAHLALKQRDAVGLTVFSDSVTAHLAPRAKPGQFDEILATLAAAE
                     VRPATDNDRALHQGAEFARRRGMVVLFSDLFGDLATIVGGLDHLRYKSHEVIIWHIMD
                     PWERDLSVDGQIRFRDLESGETLTTQSEGIRQAYRLAVDEWRQALELECRKRAIDRIE
                     LTTDDSPERALLDYLVRRAKTF"
     gene            complement(2040..>2476)
                     /locus_tag="VHS97_22940"
     CDS             complement(2040..>2476)
                     /locus_tag="VHS97_22940"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3451130.1"
                     /translation="TIKEEMLIVKGTTGTHTGEARAVLKADELLRLQELVRGVPIADS
                     VTSYAVRFVAASRPGESKEIEGIHKYLLYGASPRASQYLVLGAKARAILAGKYHVDFD
                     DVKNLVGPVLRHRLVLNFHARADGVDADAIIRRLVENVKQEA"
BASE COUNT          460 a          784 c          781 g          451 t
ORIGIN      
        1 gcgaacaaga aggcgatcaa ggccacgcat gccatccgca gcgccaataa gagccagcgc
       61 ttgagccgcc tgcgccgcgc gttgtcgcgc agcacgatct tgaggaattg gagcgtcccc
      121 aggtcgaccg tgcgggcccg ctgacggaac agcaggtgaa tgatgatcgg gatcgccagg
      181 gcggccaggg caccaagcat tccgacctga ataaagtgca taacgcctcg aaaggatatt
      241 gatattcacc cggaacccgc cccaagcgtc gtttcggtcc agatcggcgt cgcgtcactc
      301 acccgcgatg gacttggtaa atagccagta gatgtcttgc ggacctttgg aatagccccc
      361 ctgatgcgac cagatctcct tgccgtcgaa atccacgtgg atttcggcga tcgagattcg
      421 tgcgacgccg aaagaccaag ccggcggcga tgacttcgca cggcgtgctt ctacaagtaa
      481 cacaagctcc gggttcagcc cgtacgagat catgaacagg cctccatcga tcagcccgga
      541 gttcgcgtcg gcgtaacggt gtaccggctg tggtagaacg cgcagctcat agcgttcggg
      601 tctgggctgg tcttggggtg cgaagaactc atatgccttg acctgtcgca ccagttccct
      661 catttgcctc aatcgcgtcg tcgcgtcctc gccgggcgtt ggcgccttgg ggagatcccg
      721 catgacgacc cccgcgctcg ctggttgcca cgcgccaatc tcggggacag tggccgagat
      781 ctgtcccggc gccagtgacg tcagctcggc aagccatagg tagcctcccg tggtcgcccg
      841 ctgcttcgcc aaggtcaaca gtgcggcggg ccggcccgat cggccccagg cccagaccgt
      901 accgtcgaaa aaccgccgcg ccggatcgtc gaaacggtag atgggctcgg ccaagcgctc
      961 caacttctgt ggcggcgccg atgaaacgat ctgcatcttg acgccgtcgg cgagttcgcg
     1021 catcgcgcgg agccgctcgg cctgactgtc ggcgccttgg ggcccgtccc ccgcgaccgc
     1081 gaagctcagg atcagaatcg gagaaatcag caggcgcacg gctcacctct ttccgttcag
     1141 aacgttttcg ctcgacggac caggtaatcc aggagcgccc tttcgggcga gtcgtccgta
     1201 gtcagctcga tgcgatcgat ggcgcgcttg cggcactcaa gctcgagcgc ctgccgccac
     1261 tcgtcgacgg ccagccggta cgcctggcgg atgccctcag actgcgtggt caaggtctca
     1321 ccactctcca gatcgcggaa gcggatctga ccgtcgaccg acaggtcgcg ctcccaggga
     1381 tccatgatgt gccagatgat cacttcatgg ctcttgtagc gcagatggtc gaggccgccg
     1441 acgatcgtgg ccaggtcgcc gaacaggtcg ctaaagagaa cgaccatgcc gcgacgtctg
     1501 gcaaactcgg cgccttggtg cagggcgcgg tcgttgtcgg ttgccgggcg gacttcggcg
     1561 gcggcgagcg tcgccaagat ctcgtcgaac tggcccggct tggcgcgagg ggctaggtgc
     1621 gccgtcaccg agtcggagaa cacggtcaga cccacggcgt cgcgctgttt cagggccagg
     1681 tgtgcaagcg ctgccgccag cgacgccgcg taatgcagct tgctccggcc agtgttggca
     1741 caggccatcg aggcactggc atccaagagc aggtagaggt tcaggttggt gtcggcatca
     1801 tattccttga tgtaaagccg atcgtgccgc gcgtacagct tccagttcaa gtgccggagg
     1861 tcatcgccag gtgtgtattc gcggtgcgtg gcaaattcga cggagaggcc caagtagggg
     1921 ctgcggtgca ggccgtgcat cgacccctcg acgatcgacc gggcgcgcaa ctcgatcgat
     1981 tcgagtcgtg agaaaaacgc ggggtcgatc aatgatcgag gatcgaaggg catctaggct
     2041 caggcctcct gtttgacgtt ctcgaccagg cggcgaatga tcgcatcggc gtccacgccg
     2101 tcggcgcggg cgtggaagtt gagcaccagg cggtgccgga gaaccgggcc aaccagattc
     2161 ttgacgtcgt cgaaatcgac gtggtacttg ccggcgagga tcgccctcgc cttcgcgcct
     2221 aaaaccaggt actgcgaggc ccgcgggctc gctccgtaaa gcaggtactt gtgtatgccc
     2281 tcgatttcct ttgactcacc cggccgcgag gccgcgacga atcgcacggc ataggacgtg
     2341 accgaatcgg caatcggtac tcctcgcacc agctcctgaa gccgcaacag ctcgtcggct
     2401 ttgaggactg cccgcgcctc acccgtatgc gttccggtcg tccccttcac gatcaacatc
     2461 tcctctttga tcgtgg
//
LOCUS       DATAQL010001333         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2121839, whole genome shotgun sequence.
ACCESSION   DATAQL010001333 DATAQL010000000
VERSION     DATAQL010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121839"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1288)
                     /locus_tag="VHS97_22945"
     CDS             complement(212..1288)
                     /locus_tag="VHS97_22945"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX3451131.1"
                     /translation="MRNVKLLFVIFLLLAACPAFAQQRRKIIIDQDCSGPGGSNMQTL
                     LTLIQSPAVEVLGITVVSGNQWRDEEIARTLRLLEIIGRTDIPVVPGAVFPLVHRRKE
                     AQLWQATYGKVAFAGAWDDRWWHEHDVIPKLIEGEPHTKASDEDAAHFLLRMVHQYPH
                     EVTIYEGAPMTDLALANAIDSHFVELAAGLVFMGGSLEPQTDDPEFLSAPRHEFNFWF
                     DPEAAKSVLVAPWKTIICTPVDISVKTRITDAMVAEINKSNNAAAQYVVKYFMKDQGG
                     YYMWDELAAAAWIDPTLITRKVARYMSVNVDHGPTYGDTLTWTEKDNPGLGARLVEIQ
                     MDVDLARFNKMFVDLMKAPTPQAH"
     gene            complement(1405..>1746)
                     /locus_tag="VHS97_22950"
     CDS             complement(1405..>1746)
                     /locus_tag="VHS97_22950"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3451132.1"
                     /translation="GRIIMIGSCVGERMMTPGLAAYSTTKGAVKMFTQGLSRELGDRA
                     ITVNNVQPGPIDTDLNPAAGDWATPQKAMTALNRYGTVDEVASLVAFVASPEASYITG
                     ANLTVDGGTNA"
BASE COUNT          341 a          478 c          552 g          375 t
ORIGIN      
        1 cggggcacgt gcgttcatcg cttgcggccc ggataattac gtctctgtcg tggaccttaa
       61 aacattggaa gtcacgggga ctatcgatgt cggtggggag ccggacgggt tggcgtgggc
      121 agtaaagtag ccccggacgc aatcggtgcg caacgagact ctcgggcccc aaggcttgcc
      181 agaatcggct ctggcgaact gtcagcgtta ctcagtgggc ttgcggcgtg ggcgccttca
      241 tcaagtcgac gaacattttg ttgaatcttg ccaggtccac atccatctgg atttccacga
      301 ggcgcgcgcc aaggccggga ttgtcttttt cagtccaagt taacgtgtcg ccataggtgg
      361 gaccgtggtc tacgttcacg ctcatgtaac gcgcgacttt tctggtgatt agcgtcggat
      421 cgatccacgc ggccgcggcc agttcatccc acatgtaata gccgccctga tccttcatga
      481 agtatttcac gacgtactgc gctgcggcat tgttgctctt gttgatttcc gcaaccatag
      541 cgtctgtgat tctggttttg acggagatat ctaccggcgt gcaaatgatc gttttccaag
      601 gagcgaccag cacgctcttc gcggcttcgg ggtcgaacca gaagttgaat tcgtgccgcg
      661 gcgcgctgag aaattccgga tcgtcggttt gcggctcgag gcttccgccc atgaagacga
      721 ggcccgcggc gagttcgaca aagtgagaat cgatggcatt ggcgagcgcc agatccgtca
      781 tgggcgcgcc ctcatagatg gtcacttcgt gcggatactg gtgcaccatg cgcagcaaga
      841 agtgcgcggc gtcttcgtcg gaagctttgg tatggggttc gccttcaatc agtttcggaa
      901 taacgtcgtg ctcgtgccac cagcgatcgt cccacgcgcc ggcgaaggcg actttcccat
      961 aggtggcttg ccacaactga gcttccttgc ggcgatgcac gagcggaaac acagcgccgg
     1021 ggacgacggg gatatcggtg cggccaataa tttcgagcag acgcagtgtc cgcgcgattt
     1081 cttcatcgcg ccactggttc ccgctgacaa cggtaattcc caaaacttcg accgcgggag
     1141 actgaatcaa ggtcagcagc gtttgcatgt tgctgccgcc gggaccggaa caatcctggt
     1201 caatgatgat tttgcggcgt tgttgcgcga aagccggaca agccgccagc agaaggaaaa
     1261 ttacaaaaag cagttttacg tttcgcatga gagaggagag tataaccaca aatccgcggt
     1321 gaacgggtgg tcgcgaagtc ggcgctggga tgcagatgtc cagtttggcg tggtcggagg
     1381 ttaaaggatg cgtgccgggg ggtgtcaggc gttggtgccg ccgtcgacgg tcagattcgc
     1441 gccggtgatg taggacgcct cggggctcgc cacgaacgcc accagcgagg caacctcgtc
     1501 aacggtgccg taacggttga gcgcggtcat ggccttctgc ggcgtggccc aatcgcccgc
     1561 ggcggggttc aagtccgtgt cgataggccc cggctggacg ttattgacgg tgatagcgcg
     1621 gtcgcccagt tcccgtgaca gaccttgcgt aaacatcttg acggctccct tggtggtgga
     1681 gtaggcggcc aagccgggcg tcatcatgcg ctcaccaacg cacgagccaa tcatgatgat
     1741 gcggcc
//
LOCUS       DATAQL010001334         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2429657, whole genome shotgun sequence.
ACCESSION   DATAQL010001334 DATAQL010000000
VERSION     DATAQL010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429657"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="otsB"
                     /locus_tag="VHS97_22955"
     CDS             complement(<1..607)
                     /gene="otsB"
                     /locus_tag="VHS97_22955"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX3451133.1"
                     /translation="MDPETTEPSNRSVGDLPRVLDDLLQLGERLPGAESVLLFLDFDG
                     TLAPIVDDPTLAKLPAATRQTLNELAALENVTIAIVSGRALGDVKGRVGMPGLIYAGN
                     HGLEIEGPGLSFEHPVAAGLRRKVRQITERVAAKAACLDGVEIESKGLSSSVHFRRSS
                     VAAQIELDLVIREIIADDDPDIAIAAGKMVHEIRPRVGWDKG"
     gene            complement(608..2089)
                     /locus_tag="VHS97_22960"
                     /pseudo
     CDS             complement(608..2089)
                     /locus_tag="VHS97_22960"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(2520..>3131)
                     /locus_tag="VHS97_22965"
     CDS             complement(2520..>3131)
                     /locus_tag="VHS97_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3451134.1"
                     /translation="IMPPLFRWAGRMELPGTSTAMALIAAFGLAWLAEQCGSALIIGA
                     FAAGLLVAAVPQAHQIERGITEIGHFFVPLFFVSVGASVDLKALDPFHPASRFALATG
                     GVLIVMGVAGKLVAGYAPFWFRGNKTVIGVGMVPRGEVGLIFAQTGLAAHVFDAGLFG
                     AATLMVMVTTLLAPPLLKRLLGPAPDPIPDKELEVIEDLVVGA"
BASE COUNT          672 a          936 c          893 g          630 t
ORIGIN      
        1 ttcccttgtc ccagccaacg cgcgggcgga tttcatgaac catctttcct gccgcgatcg
       61 cgatatccgg gtcgtcgtca gcaattatct ctctaatgac caagtcgagc tcgatctgag
      121 ccgcgacgct cgaccgccgg aagtggaccg aagagctcaa acctttcgac tcgatctcaa
      181 ctccatccag gcacgcggcc ttggccgcga cccgctcggt gatctggcgc acttttcgcc
      241 tcaggcccgc ggcgaccgga tgctcgaacg acagacccgg tccttcgatt tctagcccat
      301 gattgccggc ataaatcaaa cccggcatcc ccacccgtcc cttgacgtct cccagcgcgc
      361 ggccgctcac gatcgcaatc gttacgttct caagtgccgc cagctcgttc aacgtctggc
      421 gcgtcgccgc cggcaacttg gccagcgtcg gatcgtcgac gatgggcgcc agcgtgccgt
      481 cgaaatccaa aaacagcagc acactttcag ccccggggag ccgttcgcct aactgcaaaa
      541 gatcgtcaag gacgcgtggc agatcgccga ctgatcggtt cgatggttcc gtggtctcag
      601 ggtccattca ggatgcctct ggtatttcaa ttttgagtaa agcggacagg atcttgcccg
      661 cccagcgata gacgttgttg tcttgtacgg ccgatcgcat gcgctgcatg cgccgcttac
      721 gttcggtgtc aggcatctcc accgcctgat ggatcgcgtc gaccatctcg tcagtcgcga
      781 acggattgaa gaggatggca tcggtcaact cgactgccga acccgcaaac tggctcaaga
      841 gcaaaatgcc gtcgtcatca atccggctgg cgacaaactc cttggcaacc aggttcatac
      901 catcgtgcag agaactgaca atgcagatat tggcgatccg gtgcagcgcg gtcatttcga
      961 ccatcgagaa gtggcgtttg aagtagatca agggccgcca tttcgatgta ctccacttcc
     1021 agttgatgcg ttcgaccagg cagtcgatct cctcgtcgac cgcccggtac tgagggatat
     1081 ggcccctggt ggggactccg acctggatgt atgtcagccg ctcgcggtaa tcgggaaaac
     1141 gctcgaaaaa acggtcgagc gcttgcagcc gttcgggtat tcccttggta tagtcgattc
     1201 gctcgatgcc gatcgcgact aagtcacctc gctgccgaag ctggcgctcg agcttcttta
     1261 tttcctgttc gacctcggtg ctgagtgcca ctgccgcctg ccgctcaaaa tcgatgctaa
     1321 tcggaaacgg ccgtaccaag gtctacttgt tgttgcgcac gatctcggat tgctcgtggt
     1381 cgacgcgcgc ctcgatcgtg cgctcaaccg tctcgatgaa attctgacag tgatatctca
     1441 gatgaaaacc taagagatcg ttgccgagca gtccatctag cagttcttcc ttccagggaa
     1501 aaacgcgaaa tgtctcgcgg ttgggccatg gtatgtgcca gaactgggcg atgatcaggt
     1561 tcgggttgcg atctttcaag tagcgcggca ggagagcaaa atggtagtcc tggatgaaca
     1621 caaacgtggg ccgatcgtcg gcttcctcta gaacggcatc accaaagagt gcattgacct
     1681 tgcggtaagt ctcccaatct ttggaattga aaaccggacg agtgaaggtg acgtggcaga
     1741 ggggccagag cccttcattg gcaaggccgt gataatagcc ctcttcctct tccttggtca
     1801 accagacgcg gcgcaaagtg taagagggat cctcgggagg gaccgcgaca tgagcggcct
     1861 cgtcgacagt ggcacgatcg gcatcccccg agccgtgccc aacccacacc ccgccggcgg
     1921 ctcgcatcac cggatcaaga gccgccgcca tgccgctggc aggacgctgg cactcgatcg
     1981 agtcccccgc gaatcgatga atgtagggct cgcgatttgc aacaacgatc atctgatagt
     2041 cggtcattct tgtcgtgatg agatcatgta acgatgcctt atcccacatg tttcgccctt
     2101 ttgtgtaaga gttgccgccc gtggtaggta ttggttgagt cacccgtgca ctgccgaatc
     2161 gctcaacgca tgcatcgctc agatcgggcg ggaaagcttt aaaccgtgtc tcggctgacc
     2221 atccgggtca gagtggcacg atcccggctc ctcggggagg ttccgacata aatcggagtg
     2281 atccaaggtc ttacttggca ggatcgctag ttccctgacc gtcatgaccg caaacatgac
     2341 ggcgcatccg tggaaataag ccgcggttgc ttggttgata tgcctttgac aactgtggcg
     2401 tcgcagtaat catcattgtg agcttggcca cgcaaaattc ataccagaac caaaaaaagt
     2461 ggcgacacat gacggccttg caccttgctc ggctggccgc cggatgaggc gtggttcgct
     2521 tatgccccga cgaccagatc ttcaatcacc tcaagctcct tgtctgggat cgggtcgggg
     2581 gccggtccca agaggcgctt gaggagaggc ggggccagga gagtggtgac catgaccatc
     2641 agggtggcgg cgccgaagag tcccgcgtca aagacatgag cggcgagccc cgtctgagcg
     2701 aaaatcaacc cgacctcgcc acgaggaacc attccaacgc cgatcacggt cttgttgccg
     2761 cgaaaccaga agggagcata gcccgcaacg agcttacccg caactcccat cacgatcaaa
     2821 acgcctcctg tggccagtgc gaaccggctg gccggatgga atgggtcgag ggccttcaga
     2881 tcgaccgaag ctccgacaga gacgaaaaaa agcggtacga agaagtgacc gatctcggtg
     2941 atcccgcgtt cgatttggtg ggcttgtgga accgccgcga cgagcagccc ggcggcgaac
     3001 gcgccgatga tcagcgccga gccgcattgc tcggccagcc aggcgagccc aaatgcggcg
     3061 atcaaagcca tcgccgtcga cgtgccagga agctccatcc ggccggccca acgaaacaaa
     3121 ggcggcatga t
//
LOCUS       DATAQL010001335         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2884837, whole genome shotgun sequence.
ACCESSION   DATAQL010001335 DATAQL010000000
VERSION     DATAQL010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884837"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VHS97_22970"
     CDS             <1..1107
                     /locus_tag="VHS97_22970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX3451135.1"
                     /translation="LEACADNPPDVEDRGSPREAMSAGSPVSLRDFERLASGVMTRPA
                     WEFISSGAGDEQTVRWNEEAFGRLRLRQRVLEDVSRLDTRIRLLGRERPHPILLAPTS
                     NHTLVHPDGEQATARGAGSAGATMVVSTFADKSLEEIARAATQPLWHATYVMKDRGRT
                     QDLLRRAEAVGCEAICVPVDSPVVGARDREHRTYRFPREPVSFLDYPVDYWRFPTTWK
                     DIDWIRSQTKLPLVLKGILDPADAELGIRAGAQAIFVSNHGGRNLDTLPATLEVLSDV
                     ADKVAGRVPILVDGGVRRGTDVLKALALGASAVAIGRPYLYGLACSGAAGVRAVVNIL
                     RNELEMAMALTGRPTIASIDRSVVHPTPSGPSRS"
     gene            1405..2169
                     /locus_tag="VHS97_22975"
     CDS             1405..2169
                     /locus_tag="VHS97_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX3451136.1"
                     /translation="MPDPVAPPSDCPPKLLISLATFNEAGNLKPLVETIRQVAPASAI
                     LVIDDNSPDGTGSIADELRDTLPNFDVIHRPGKLGLGTATLAAVRYAIDHHFDYLLNL
                     DADFSHPPRFIPDLLKGMADHDVMIGSRYVPGGGVEGGFNLKRRLMSSGINGYARLLL
                     GLASKDNSGAFRCYRVSKLAELDLDLVRSRGYSFQEEILYWCKTVGCRIGETPILFEN
                     RRAGSSKINMREAVSALWILLLLGTARMTGSISKKS"
     gene            complement(2185..2547)
                     /locus_tag="VHS97_22980"
     CDS             complement(2185..2547)
                     /locus_tag="VHS97_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451137.1"
                     /translation="MNLARNGANGNPGMLEVDDADDDMDDVGGTETRAGAASPELKRS
                     LADIKGQAQFLLYLADQIEESLDQLATEADACHGVFLCKVLGMYSGQLESKHQGLGEK
                     IAETCQEVYVTVREREMV"
BASE COUNT          463 a          858 c          818 g          516 t
ORIGIN      
        1 ctggaagcat gcgccgacaa tccgccggat gtcgaggacc gggggtcgcc gcgcgaagcg
       61 atgtcggccg ggagccccgt cagcctccgc gatttcgagc gcctggcgtc cggggtgatg
      121 acgcggccag cctgggagtt catcagcagc ggcgccggcg atgagcaaac ggtgcgctgg
      181 aacgaggagg cgttcggccg gctccggctc cgccagcggg tcctggagga cgtctcacgg
      241 ctggacacgc ggatccggct gttgggccgc gagcggcccc atccgatcct cctcgcgccg
      301 acctccaatc acaccctggt ccaccccgat ggggagcagg ccacggcgcg cggggcgggc
      361 tccgccggcg cgaccatggt cgtcagcacc ttcgccgaca agtccctgga ggagatcgcc
      421 cgggcggcca cccaacccct ctggcacgcc acctacgtca tgaaggatcg cggccgtacc
      481 caagacctgc tgcggcgcgc cgaggccgtc ggctgcgaag caatctgcgt tcccgttgat
      541 tcccccgtgg tcggcgcccg ggatcgcgag catcgtacct accggttccc gcgcgagccg
      601 gtgtcctttc tggactaccc ggtggattac tggcgtttcc cgacgacctg gaaggacatt
      661 gactggattc gctcccagac caagctcccg ctcgtgctca agggcatcct cgaccctgct
      721 gacgccgagc tgggaatccg ggcgggggcg caggcgatct tcgtctcgaa ccatggcggg
      781 cgtaacctgg atacacttcc ggccacgctg gaggtcttat ccgacgtcgc cgataaggta
      841 gcagggcgcg tcccaatcct ggtcgatggc ggcgtgcggc gaggtacgga cgtgctcaag
      901 gccctggccc tgggagcctc ggccgtggcg atcgggcgac cctacctcta tggactggcc
      961 tgctcgggcg ccgcgggtgt ccgggctgtg gttaacatct tgcgcaacga actggagatg
     1021 gcgatggcat tgaccggccg tcccacgata gccagcatcg accggtcggt cgtccatccc
     1081 acgccgtccg ggccctcgcg aagctgattt gctgccattt atgtgtttct cgaccggatg
     1141 actggaccta cctgaccgta cttctgagtc taccgctctc agccgacgac gttaccttcc
     1201 cggacgaggc tgtgcgcatc tcgcgaacgc agagccgcct ggccgcaacg ctgaggtgca
     1261 ttcgcaacac aaggtgcacc tgctttcgaa ttggcttggc gcagcttgct ttcgagcgat
     1321 atcttaagca actcaccggc ttggagtgcg aatcggttcg catccacggc caattcgacc
     1381 gtctgaatcc ggaaatccat ggccgtgccc gacccagtag cgccaccgtc cgactgtcct
     1441 cccaagctgc tgatttccct ggcgaccttc aacgaggcgg gcaacctgaa gcccctggtg
     1501 gagacgattc gccaagtcgc tccggcgagc gcgatcctcg tcatcgacga caactcgcct
     1561 gacggaactg gctcgattgc tgacgagttg cgtgacactt tgcccaattt tgacgtgatc
     1621 caccggcccg gcaaactggg actgggaacg gccacgctgg cggcggttcg atatgcgatc
     1681 gatcatcact tcgattatct gctgaacctg gatgccgatt tcagccatcc gccacggttc
     1741 attcccgacc ttttgaaggg gatggccgac cacgacgtca tgatcggctc gcggtacgtg
     1801 ccgggaggcg gcgtcgaagg agggttcaac ctcaagcgac ggctaatgag ctcgggaatc
     1861 aacggctacg cccgtctgtt gctcggcttg gctagcaaag acaacagcgg ggcgtttcgg
     1921 tgctatcggg tcagcaagct ggctgagctc gacctggacc tggttcggtc gcgaggctac
     1981 tcgtttcagg aagagattct ctactggtgc aaaaccgttg gctgccggat cggtgaaaca
     2041 cccatcctat tcgaaaaccg ccgtgccggc tcgtcaaaaa tcaacatgcg cgaggccgtc
     2101 tcggctctct ggatcctttt gttgctgggg accgcgcgca tgaccggcag catctccaag
     2161 aagagttagc cgggagacaa cctactaaac catctcgcgc tcgcggacgg tgacgtaaac
     2221 ttcctgacag gtctcggcga tcttctcgcc cagtccctga tgcttgctct cgagctgacc
     2281 ggaatacatg ccaagcacct tgcacagaaa cacgccgtgg cacgcgtctg cctcggtggc
     2341 aagctggtcg agcgattctt cgatctgatc ggcgagataa agtaagaatt gggcctgtcc
     2401 cttgatatcc gccaatgagc gcttgagctc tggtgaggcc gcccccgcac gtgtctcggt
     2461 gccgccgacg tcgtccatat cgtcgtcggc atcgtccact tcgagcatcc cgggattacc
     2521 gttcgcgccg tttcttgcta agttcattgt aggtctccgc ggtcgtgcgg gtccgacgag
     2581 ccgtcgacca tggattcgaa ggcggcgcac aaatcgccta cgagtcgcat gttctggctt
     2641 tcgagtctga gattc
//
LOCUS       DATAQL010001336         9771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5175390, whole genome shotgun sequence.
ACCESSION   DATAQL010001336 DATAQL010000000
VERSION     DATAQL010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5175390"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4391)
                     /gene="gltB"
                     /locus_tag="VHS97_22985"
     CDS             complement(<1..4391)
                     /gene="gltB"
                     /locus_tag="VHS97_22985"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX3451138.1"
                     /translation="MSIGLPLAQGLYDPRYEHDSCGVGFIVDLKGRRSHSIVRDGLTA
                     LNNLNHRGACGCENNTGDGAGILIQIPHEFLVSRCLSLGISLPAPEEYGVGAFFTSPE
                     ESQQKFGMELFERLVAQEGQEFLGWRRVKTDASSLGESARQVEPVMWHAFVGRSASLG
                     DAERFERKLYVIRKRFEKEIEDSGLDDHKYFYFSSLSCRTLVYKGMLTTEQLSWYFAD
                     DLGNPLCTSALCMFHSRFSTNTFPSWQLAHPYRMISHNGEINTLRGNINWMRAREALF
                     SSDLYEPGDIAKIRPIIREGLSDTACLDNAVELLVRSGYSLPHAMMMLIPEAWENHES
                     MSQAKKNFYRYHSCLMEPWDGPASVTFTDGKTIGAVLDRNGLRPSRFVETDDGRVIMA
                     SEVGALEIAPEKIVRKGRLEPGKMFLVDLQKGQIVNDEELKHVLATAHPYGEWLNTFM
                     VPLADLPPAPEVPGPDHDTLLQRQIAFGYTLEDLKYILGPMGSNGEEAIGSMGTDTPL
                     AVLSDRAQPLFNYFKQLFAQVTNPPLDAIREELVTSVFTGAGGEGNLLSPTPEACRQI
                     ALELPILDNDELARIKQLDGWRGFKSHTLPMLFRAAEGAQGLATALESLFEQACVAIV
                     AGANLIVLSDRGVTPEIAAIPSLLACSGLHHEMVRRGLRSRAGLIIECGDAREVHHFA
                     LLLGYGAGTVNPYQAFESLDDMIRQGTISGIDHDEAVYRYGKAIKKGVVKVMSKMGIS
                     TIQSYRGAQIFEAIGLHEDFVRKYFDKTASRVGGVGLAEIAAETLHHHHRAFSSREAR
                     TSGLLEGGQYQWRRDGEHHLFNPETVFRLQHATQAGRYDIFKKYTSLVDDQNERLCTL
                     RGLFQLRFDRTKPVPIEEVESVESIVRRFATGAMSYGSISAEAHETLAIAMNRIGGKS
                     NTGEGGEDPERFKPLANGDSKKSAIKQVASGRFGVTSEYLVSAQELQIKMAQGAKPGE
                     GGQLPGHKVWPWIAKVRYSTPGVGLISPPPHHDIYSIEDLAQLIYDLKNSNPKARISV
                     KLVAEMGVGTVAAGVAKAHSDVVLISGHDGGTGASPLTSLKHAGIPWELGLAETQQTL
                     VMNKLRDRIVVQTDGQLKTGRDVVIAALLGAEEYGFATAPLVVMGCVMMRVCHLDTCP
                     VGIATQNPALRAKFQGQADHVINFFRFIAQEVRELMAQLGFHTIADMVGHSELLDMNR
                     AITHYKARGLDFSKILHRPDTGPGVPVRKMRDQEHGIESSLDVTTLIPSCLPALERGE
                     NVALDLPIRNINRTVGTILGSELTRRHGGAGLPDDTIELKFTGSAGQSLGAFLPRGIT
                     LTLEGDANDYTGKGLSGGKIIVYPPKIASFVAEENILIGNVALYGATSGRAFFRGRAG
                     ERFCVRNSGASAVVEGTGDHGCEYMTGGVAVIIGTTGRNFAAGMSGGVAYVLDAAGDF
                     RGRCNLDMVDVEALTEPDDVDLLRDLL"
     gene            complement(4580..5539)
                     /locus_tag="VHS97_22990"
     CDS             complement(4580..5539)
                     /locus_tag="VHS97_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3451139.1"
                     /translation="MQLESLKIFCDVVRWASFSRGAAENGISQSSASQAVHQLEVRLG
                     VKLIDRSKRPLVLTPPGRVYHEGCKDLVDRYLELENRVKALEDVDTVVGTVGVAAIYS
                     VGLNHMSQYVKSFEERHRGANVRLEYLHPSRVLERVNNGEAELGLLSFPRKWPDLNVV
                     TWREEAMVVTVHPSHRFARRSSIMVADLDGEAFVGFDPDLSIRRAIDRFLRRHDVQVD
                     VVLEFDNIENIKRAVEIPSGISILPEPSLAQEVKAGSLVAIPIEGTDPSDRITRPLAI
                     IHRRQAILEPAAAKFLELLIGDDGSNKTRVTAEPKRVPASMSP"
     gene            6417..7010
                     /locus_tag="VHS97_22995"
     CDS             6417..7010
                     /locus_tag="VHS97_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3451140.1"
                     /translation="MVNPHESGRTGGVDVPPAGDTGGVSKGKAFLGLFLKAQRRIFAY
                     IFTLLPNRADAEDVLQEVSKLMWEKFDEADPPGDFVAWGCRIAFYKVMEHRRNHRRDR
                     VIFSDAMLERVAETVSEHADALQLDERHDALACCLGKLSRGDRQLLDERFKDGATVRS
                     VAERLGRSADAVYKALARVRRGLHDCVERTIAAGSHA"
     gene            7007..7414
                     /locus_tag="VHS97_23000"
     CDS             7007..7414
                     /locus_tag="VHS97_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451141.1"
                     /translation="MTLEPERLNELPRLLEALCEERITPEEAARLEALVLADPRAEAL
                     YITYLTMQADLAREIGGHHAAAAGGGRRCPARRAPGPGAGPAAPVVVLGAVGPRGRGL
                     PAGGDDVGPRGLAGCRRRAIGRAPLRPGGVRAG"
     gene            7341..8732
                     /locus_tag="VHS97_23005"
     CDS             7341..8732
                     /locus_tag="VHS97_23005"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX3451142.1"
                     /translation="MAWRGAGGARSGEPRSGPAASAPGEPGTGGGRLVVHNRDAVAVV
                     MRLVDARWEPSDGPAPTEGRPVPAGRLRLRSGLATLSFVNGVTLTLEGPADVDLVSID
                     RVFCRRGKLRTRVPKGAEGFVVASPSSAVVDLGTEFALNVGDDGRARVMVFEGVAEAA
                     VLSASGAPRHSQFIERDRAFDLDAGAGRIREATVRPEAFAPAPDLRDRDLDLAPSYVR
                     EVLRSQPRGYWRFESLSDGFFPEEVADGPPLRAHGSVRAGRGPGGNGYCAFPPGDPGQ
                     YLDTEPLWDLPAEPGHAVEFWFLSEGIAYASLVGFYPPRGLNPPDQGDRFVHTFFVEA
                     TAYQRQSLQKPASVRFLHRWPLDIKVNDSLFSEGFYVPRRWHHVVAQKNDGRLELYFD
                     GQLDRSSLLESVHPTLSCHLTVGRRTPESDNPKDSRSFVGRLDELAIYDHPLSAEEVR
                     AHYRLGSESPRPD"
     gene            8843..>9771
                     /locus_tag="VHS97_23010"
     CDS             8843..>9771
                     /locus_tag="VHS97_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451143.1"
                     /translation="MRRQGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRSQCTNN
                     LKQLGLAMHNYHDSVSSFPIGAMGVRSLIAGGRYPDGTPVGNARRTWLPMLLPYIEQG
                     VVANSYNFSQIFNSYLNTTAELTQISALSCPSDPNQGSIENSPPRYQHNYVVNWGNTT
                     WQQNMTTIYNPTVGIYPPGTTNSVPFLGAPFTMDKSFGIPSITDGTSNTLLMAEVKIG
                     ADITGGIQDHRGDVWNDDWMCAMFTAFTPPNVSFNDYLGNDCNYPYQNNPPCIVKAPY
                     FIAARSYHSGGINALMADGHVQFFKNTINYIVWR"
BASE COUNT         1724 a         3188 c         3069 g         1790 t
ORIGIN      
        1 atcaagaggt ctcgtaacag gtcgacatcg tcgggctcgg tcagggcttc gacgtctacc
       61 atatcgaggt tgcagcgtcc gcggaaatcg cccgcggcat ccagcacgta cgccacgccg
      121 ccgctcatgc ccgcggcgaa gttgcggcct gtcgtgccga tgatcacggc gacgcctccg
      181 gtcatgtatt cgcaaccatg gtctcccgtg ccttcgacca cggccgaggc accgctattg
      241 cgaacgcaga aacgctcgcc ggctcggccg cggaaaaaag cgcggccact ggtcgcgccg
      301 tagagtgcca cgttgccgat caagatgttc tcttcggcaa caaaactcgc gatcttgggc
      361 ggataaacga tgattttgcc gcccgacagg cccttaccgg tatagtcgtt ggcgtcgcct
      421 tcgagggtca gagtgattcc ccgagggagg aatgcgccca aactctgccc ggccgatcca
      481 gtaaacttca gctcaatcgt gtcgtccggc aacccggcgc cgccgtggcg acgcgtgagc
      541 tcgctcccca ggatcgtgcc gaccgtacgg ttgatgttgc ggattggcaa gtcgagcgcg
      601 acgttctcgc ctcgttcgag agccggcaga catgagggaa tcaaggtcgt cacgtccagc
      661 gacgactcaa tgccgtgttc ctggtcgcgc atttttcgga ccggaacacc ggggccggta
      721 tcgggtcggt gaagaatctt gctgaaatcg aggcccctgg ccttgtagtg ggtaatggcc
      781 ctgttcatgt caagcagctc gctatgaccg accatgtcgg ctatcgtgtg aaagccaagc
      841 tgcgccatca gctcgcgcac ttcctgggcg atgaatctga agaagttgat cacgtgatcg
      901 gcctgccctt gaaacttggc gcgtaaggcg gggttctggg tcgcgatgcc gaccgggcag
      961 gtatccaggt ggcagacgcg catcatcacg caacccatca caaccaacgg ggcagtcgcg
     1021 aagccgtatt cctccgcacc caggagcgcg gcgatcacga catcgcgacc ggtctttaat
     1081 tgcccgtcag tctggacgac aatccgatcg cgcagcttgt tcatcaccag ggtctgctgg
     1141 gtctctgcca gacccaactc ccaggggatg ccggcgtgct tgagcgaggt gagcgggctg
     1201 gcgccggtgc cgccatcatg gccgctgatc aagaccacgt cgctatgggc cttggccact
     1261 cccgcggcca ctgtgccgac tcccatctcg gcgaccagct tgacgctgat tcgtgccttg
     1321 gggttagagt tcttgaggtc gtaaatcaac tgggccaggt cttcgatcga atagatgtcg
     1381 tgatgaggcg gcgggctgat caggcccacg ccgggtgtcg agtaacgcac ctttgcgatc
     1441 cagggccaga ccttgtgccc ggggagctga cccccttcgc ccggcttcgc cccttgggcc
     1501 atcttgattt gcagctcttg ggcgctgacc agatactcgc tggtgacgcc gaaccttccc
     1561 gaggcaactt gcttgatcgc gctcttcttg ctgtctccat tggcgagcgg cttgaagcgc
     1621 tcgggatcct cgccaccttc gccggtattc gacttaccac cgatacgatt catggcaatc
     1681 gccagggttt catgcgcctc ggccgagatc gagccgtagc tcatggcgcc cgtcgcaaac
     1741 cgccggacga tcgactcgac cgactcgacc tcctcgatcg gcaccggttt tgtccggtca
     1801 aaccgcaact ggaagaggcc gcgcagcgtg cagagccgtt cgttctggtc gtcgaccaag
     1861 ctcgtgtact tcttgaagat gtcgtatcgg cctgcctgcg tcgcatgctg gagacggaag
     1921 acggtctcgg ggttgaacag atgatgctcg ccatcgcggc gccactggta ttggcccccc
     1981 tccaagagcc cgctcgtccg cgcctctcgg ctggaaaaag cgcgatggtg gtgatgcaga
     2041 gtctcggcgg caatctccgc taagcccacg ccgccaaccc gcgacgccgt cttgtcgaag
     2101 tacttgcgaa cgaaatcttc gtgaaggccg atagcctcga agatctgcgc tccccgataa
     2161 ctctgaattg tgctgatgcc catcttggac atcaccttga cgaccccctt cttgatcgcc
     2221 ttgccgtagc gatagacggc ttcgtcgtgg tcgatgccag aaatcgtccc ttgccggatc
     2281 atgtcgtcaa ggctctcgaa tgcctgatat ggattaacgg tgccggcgcc atatcccaac
     2341 aggagcgcga agtggtgcac ctcgcgggcg tcgccgcatt cgatgatcag gccagcgcgc
     2401 gagcgcaacc ctctgcggac catctcgtgg tgcaaacccg agcaggcgag taacgagggg
     2461 atggccgcga tctcgggagt cacgccgcga tcggagagga cgatcaggtt ggcgcctgct
     2521 acgatggcaa cgcaggcctg ctcaaagagc gattccagtg ccgtcgccag gccttgggct
     2581 ccctcggcag cccggaagag catggggagc gtgtgcgact tgaaaccacg ccaaccgtcg
     2641 agttgcttga tccgcgccag ctcgtcgtta tcgaggatcg gtaactcaag cgcgatctga
     2701 cggcatgcct cgggagtcgg cgagagcaag tttccctccc caccggcgcc cgtgaaaacc
     2761 gatgtcacaa gctcttcgcg aatcgcgtcc aaggggggat ttgtgacctg ggcgaatagc
     2821 tgcttgaaat aattaaagag aggctgcgcc cggtcggaaa gtaccgccag cggcgtgtcg
     2881 gtccccatag accctatcgc ctcttcgcca ttagacccca tcgggcccag gatgtacttc
     2941 aagtcttcga gcgtgtagcc aaacgcgatt tgccgctgca gtagggtgtc gtggtcgggg
     3001 ccagggacct ccggcgccgg cggcaagtcg gccagcggca ccatgaacgt gttcagccac
     3061 tccccataag gatgcgctgt ggcgaggacg tgctttaact cctcgtcgtt gacaatctgt
     3121 cccttctgca ggtctaccag gaacatcttg cccggctcca atcgcccctt cctgacgatc
     3181 ttttccggag cgatttccag ggcgcccacc tcggacgcca tgatcacgcg gccgtcgtcg
     3241 gtctcgacga atcggctggg acgcaggccg ttgcgatcga ggaccgcgcc gatcgtcttg
     3301 ccgtcggtga acgtgaccga cgcggggccg tcccaaggtt ccatcaggca actatgatat
     3361 ctgtagaagt ttttcttggc ctgagacatc gattcgtggt tttcccaggc ctcggggatc
     3421 aacatcatca tggcgtgcgg caggctgtat cctgagcgga ccaacagctc aactgcgttg
     3481 tcgaggcatg cagtatccga gagcccttcg cgaatgatcg gccggatctt ggcgatgtcg
     3541 cccggttcgt aaagatccga tgaaaacaag gcctcgcgcg ccctcatcca gttgatgttt
     3601 ccccgcaacg tgttgatctc gccgttgtgc gagatcatgc gatacgggtg ggcgagctgc
     3661 cagctcggga acgtgttggt gctaaagcgc gaatggaaca tgcacagagc gctcgtacat
     3721 agcgggttgc cgagatcgtc ggcgaaatac cagctaagct gttcggtcgt gagcatcccc
     3781 ttgtagacca gggtgcggca cgacaggctc gaaaaataga aatacttgtg atcatcgagc
     3841 cccgagtctt cgatctcttt ctcaaaccgc ttgcggatga catagagctt gcgctcgaac
     3901 cgctcggcgt cgcccaggct ggcgcttctg ccgacgaaag cgtgccacat caccggctcg
     3961 acctgccggg cgctttcacc cagcgaactg gcgtcggtct tgacgcggcg ccagcccaga
     4021 aattcttgcc cctcttgagc gacgagtcgc tcgaaaagct ccatgccgaa tttctgttgc
     4081 gactcttccg gcgacgtgaa gaatgcgccc acgccatact cttccggcgc gggcagcgag
     4141 atcccgagcg acaggcaacg cgagacgaga aactcgtggg ggatctggat caagatcccc
     4201 gctccatcac cggtgttgtt ttcgcacccg caggcgccgc gatggttcaa attgttgagg
     4261 gcagtcaagc catcacggac aatactatgc gaccgccgtc ctttgagatc gacgatgaag
     4321 cccacgccgc agctatcgtg ttcgtagcgc gggtcgtaca gcccctgggc aagcggtagg
     4381 ccgatactca tctctggttg tccttggcta gcctgacatg cacacaaaat gatcgcagga
     4441 ggtgatgcgg aaatgccagc gcatcgcgtg ccgtatctgg gggaaaaggt ctgtccatcg
     4501 gggcggttgt tctcgttcta gcggtaaaga cggcttcgcg cggagcaatc ctgccggcca
     4561 cgactcgccc taacaaaact cagggagaca tactggcggg aacgcgtttc ggctcagccg
     4621 taacccgggt cttgttggac ccatcgtcgc cgatcagtag ttccagaaac ttggccgcag
     4681 caggctcgag gattgcctgc cggcgatgaa tgatcgccag tgggcgagta atgcggtcgc
     4741 tcgggtccgt tccttcgatc gggatcgcga ccaatgaccc cgccttgacc tcttgcgcca
     4801 atgacggctc gggaaggatg gagatgcccg aagggatctc gaccgcgcgc ttgatgtttt
     4861 cgatgttgtc gaactcgagc accacgtcca cctgaacatc atgacggcgc aaaaagcggt
     4921 cgattgcccg ccggatcgac agatccggat caaagccgac gaacgcttca ccatcgagat
     4981 ccgccaccat gatgctggaa cggcgggcga atcggtggga cggatgaacg gtgacgacca
     5041 tcgcctcctc gcgccaggtg acaacgttca ggtcgggcca cttgcgcgga aatgacaaca
     5101 agccgagttc cgcctcaccg ttgttcacac gttcgagtac gcggctcggg tgcaaatatt
     5161 cgagccgaac gtttgccccg cgatgacgct cttcgaaaga tttgacgtac tggctcatgt
     5221 ggttgagccc gaccgagtaa atcgcggcga cgcccaccgt gcctacgacg gtgtccacat
     5281 cctcgagagc cttgacccgg ttttccagct cgaggtaccg atcgaccaga tccttgcagc
     5341 cctcgtgata aaccctgccc ggtggcgtga gcacgagcgg ccgtttggaa cggtcgatca
     5401 acttcacccc gagccgcacc tcaagctggt gaacagcctg gctcgcggag gactgcgaga
     5461 ttccgttttc cgcggccccc cgcgaaaaac tcgcccaccg gacgacatca cagaaaatct
     5521 tcaacgactc gagctgcata cgttccttgc ctatcggcat ttccttgata atgcgctgaa
     5581 aaccttattg gaatctgtaa tagtagagat catcgttcgc aggagtcaat agggatgcac
     5641 ctactttttg gttgtagttg cctattttca cgtcgtcgcg gccgtcgctt gacactggcg
     5701 acatacaaca acctgcgtcg gatttgtaag tttcgcctca ccgatttaca aaggtgcagg
     5761 atcggatcat cgtcctcccg aacgtcggct gtttgcgcgg tggtggcccg cctcattcgc
     5821 gccgacttcg ggtgccacgc gtgtgccagc atttccgtcg cagatggact ttgagttcgt
     5881 tcgcgccgct tccgagttcg atacgtgcgc ccgcaaatcc ggcacagatg gactttgggt
     5941 tcgttcgcgc cgtttccgag gggatcgtcc caaatcgtcc gcttggggcc tgagtgagct
     6001 ggacggttgc ggccaggtgt acgcgcggcg acccaacgtc acaatccccg cgcaggctaa
     6061 gggttaaatg cgccgaactc ccggcgcttc ggggtcagca aagtgtcaag ttctaaatcg
     6121 gcggttaggg acgatcccca aaaaggcgag cggcgttttc cgcaatcggc gacggcggaa
     6181 ggacttgggc cgagcggcgg gccaacctga cgggccgccg gatttttcgc ttccaaaaag
     6241 cggcccggcc ggccacttct ctacaaagcc cattctccag caggtcaacc gcagtcgtcg
     6301 ccgcacaccg gggggtgtct ccgccgcgcc gacgagcctc cgtcagccgg gactgctccg
     6361 gtcgtcacgg cgggcgcctc gtgtcgtgcg cgaggcgcat tgaagggtcg agcgtcatgg
     6421 tcaatccgca cgagtccggg cgtactggcg gcgtcgacgt cccgcccgcg ggggacacgg
     6481 ggggggtgtc gaaggggaag gcgttcctcg gcctgttcct caaggcgcag cgtcggatct
     6541 tcgcctacat cttcaccctg ctgcccaacc gcgccgacgc ggaggacgtg ctccaggagg
     6601 tcagcaagct gatgtgggag aagttcgacg aggcggaccc gccgggggac ttcgtcgcgt
     6661 ggggttgccg gatcgccttc tacaaggtga tggagcaccg gcggaaccac cggcgagatc
     6721 gggtgatctt cagcgacgcg atgctggagc gggtggccga gacggtgtcg gagcacgccg
     6781 acgcgctgca gctcgacgag cgccacgacg cgctggcgtg ctgcctcggc aagctgagcc
     6841 ggggcgaccg gcaactgctc gatgagcggt tcaaggacgg ggccacagtc cggtcggtcg
     6901 ccgagcggct agggcgatcg gccgacgcgg tctacaaggc ccttgcgcgg gtccggcggg
     6961 gcctgcacga ctgcgtcgag cggacgatcg ccgcggggag ccacgcatga ccctcgagcc
     7021 cgaacgcctg aacgagttgc cgaggctgct ggaggcgctc tgcgaggagc ggatcacgcc
     7081 cgaagaggcc gcgcggctgg aggcgctggt gctggccgac ccgcgggcgg aggcgctgta
     7141 catcacctac ctgacgatgc aggccgacct ggcccgcgag atcggcgggc accacgcggc
     7201 ggcggccggc ggggggaggc gatgcccggc ccgacgtgcc cccggccccg gagccggccc
     7261 ggcggcgccg gtcgtggtcc tgggtgccgt gggccctcgc ggtcggggcc tgcctgctgg
     7321 cggcgacgac gtcgggcctc gtggcctggc ggggtgccgg cggcgcgcga tcgggcgagc
     7381 cccgctccgg cccggcggcg tccgcgccgg gtgagcccgg caccggcggc ggccggctcg
     7441 tcgtccacaa tcgcgacgcc gtggccgtgg tgatgcggct ggtcgacgcc cgctgggagc
     7501 cgtcggacgg cccggcgccc acggagggga ggcccgtccc ggccggccgc ctgcggctcc
     7561 gatcggggct ggcgacgctg agcttcgtca acggggtcac gctcaccctc gagggccccg
     7621 ctgacgtcga cctggtgtcg atcgaccgcg tcttctgccg ccggggcaag ctccggaccc
     7681 gagtgccaaa gggggccgag ggcttcgtcg tcgcgtcgcc gtcgtcggcc gtggtcgacc
     7741 tgggcaccga gttcgccctg aacgtcggcg acgacggccg ggcccgcgtg atggtgttcg
     7801 agggggtcgc cgaggccgcc gtgctcagcg cctcgggcgc gccccggcac tcgcagttca
     7861 tcgagcggga ccgggcgttt gacctcgacg ccggggccgg ccgcatccgg gaggcaacgg
     7921 tccggcctga ggcgttcgcc ccggcccccg acctccgcga ccgcgacctg gacctcgcgc
     7981 cctcctacgt tcgggaggtc ctgcggtccc agccgcgcgg ctactggcgg ttcgagtcgc
     8041 tgagcgacgg cttctttccg gaagaggtcg ccgacggccc gccgctccgc gcccatggct
     8101 ccgtccgcgc ggggcgcggg cccgggggca acgggtactg cgctttcccg ccgggcgacc
     8161 ccgggcagta cctggacacc gagccgctct gggacctccc cgccgagccg gggcacgccg
     8221 tcgagttctg gttcctctcg gaggggatcg cctacgcctc gctcgtgggc ttctacccgc
     8281 cgcggggcct gaaccccccc gaccagggcg accgtttcgt gcacacgttc ttcgtcgagg
     8341 ccacggcgta ccagcgccag tctcttcaga agccggcgtc ggtccggttc ctgcaccgct
     8401 ggccgctcga catcaaggtc aatgacagcc tgttctcgga gggcttctac gtcccccgcc
     8461 gctggcacca cgtggtcgcg caaaagaacg acggccggct ggagctgtac ttcgacggcc
     8521 agctcgaccg gtccagcctc ctggagtccg tccacccgac cctcagctgc cacctgaccg
     8581 tcgggcgcag gacgccggag tcggataacc cgaaggacag ccgctccttc gtcggccggc
     8641 ttgacgagct ggccatctac gatcacccgc tctcggccga ggaggtgcgc gcacactacc
     8701 gtctcggctc ggagagcccg cgtcccgact gatccgatcc gctcaccgcc gggccggccg
     8761 ccaggcccga tccgtatcga gtctgtccca caggtcgtgt gggggcctgc acgtcgtttc
     8821 ccgtcccgaa ggaggtttcg ccatgcgcag acagggtttc acgctgatcg agctgctcgt
     8881 cgtcatcgcc atcatcgccg tgctgatcgc gctcttgctc ccggccgtgc aggcggcccg
     8941 cgaggccgcc cgccggtcgc aatgcaccaa caacctcaag caactcggcc tcgcgatgca
     9001 caactatcac gactcggtca gcagctttcc gatcggcgcg atgggggtgc ggagcctgat
     9061 cgcagggggc cggtacccgg acgggacgcc cgtgggcaac gcccgccgga cctggctccc
     9121 gatgctcttg ccctacatcg aacagggggt cgtggccaac tcctacaact tcagccagat
     9181 cttcaactcc tacttgaaca cgaccgccga gctgacccag atcagcgccc tatcgtgccc
     9241 ctccgacccg aaccaggggt cgatcgagaa ttcccccccg cggtaccaac acaactatgt
     9301 ggtcaactgg gggaacacga cctggcaaca gaacatgacg acgatctaca accccacggt
     9361 cggcatctac cctcccggca cgaccaactc ggtcccgttc ttgggggcgc cgttcacgat
     9421 ggacaagtcg tttgggatcc cgagcatcac cgacgggacg agcaacactt tgctgatggc
     9481 cgaggtgaag atcggcgccg acatcaccgg cggcatccag gaccaccgcg gcgacgtctg
     9541 gaacgacgac tggatgtgtg cgatgttcac ggcgttcacc ccccccaacg tctccttcaa
     9601 cgactacctc ggcaacgact gcaactaccc ctaccaaaac aacccaccct gcatcgtcaa
     9661 ggccccctac ttcatcgccg cgaggagcta ccactcgggc ggcatcaatg cgctcatggc
     9721 ggacgggcac gtgcagttct tcaagaatac gatcaattac atcgtctggc g
//
LOCUS       DATAQL010001337         5344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5175406, whole genome shotgun sequence.
ACCESSION   DATAQL010001337 DATAQL010000000
VERSION     DATAQL010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5175406"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2062
                     /locus_tag="VHS97_23015"
     CDS             <1..2062
                     /locus_tag="VHS97_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX3451144.1"
                     /translation="QFHQVVLNDLAPTPPASGLLVVDLDADGRADLAAPSAEGHVYAW
                     RNATERTTTTQTRLTFEPWPTNASRWRSAQAIDLDLDGRTDLLGLLASSSKVSEFDVP
                     LWARNERALLATKTLPVQLESPGLDGLIAVDLVGDPLPDILAVRPGSPPVLARNLGNG
                     RHWLAFELGGLWHVKPELMRTNSHGLGTRVLVEGQGTHVTHEHTTQETGLGQSVVPFV
                     LGLGERERAELIHLRWPDGVMQCELNVTGDQKLSLVEKNRKTGSCPVLFTWNGRRFVC
                     IGDFLGGGGLGYLVAPGVYGQPDRDEAIAVTAEQLQPSNGVLRISITEPMDEVAYLDH
                     LRLDAIDCPPGVSATPDERFAPDGTRPTGELIAWRAAIEPVRATDLEGRDLTEILRYW
                     DRRTADLFRKRDGWIGYSEEHAIILDFGDRLSRFGPSDRLVLCLAGWVEYPYSQTNYA
                     AATAGVAILPPAIERRREDGRFETIERNAGYPAGMPRLMTVDLTGKLTGPKCVLRIKT
                     NMECYFDQAFIVARDRFAESSLRVSSLPVARAELGHRGYTREISPDGRQPLIYDYDYV
                     DPAPLASFAGKLTRYGDVAPLLRSDDDQLCLVGPGDEVRIEFQASTLPPLAPGWTRRY
                     VVRSFGYCKDADPFTATSDTIEPLPWRTMPAFPFGAGVKRPSDPAYETYLRTYQIRPA
                     GGGN"
     gene            complement(2110..4587)
                     /locus_tag="VHS97_23020"
     CDS             complement(2110..4587)
                     /locus_tag="VHS97_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3451145.1"
                     /translation="MARRGTKSPVPPRSRPAERPAPTRGSAEDDGRDRAEEATRLGEN
                     LRRLALGLTAALVTARAFWPSEPDLKEGAGRGLLWVLSVFVVFGLALAAALIGGRFRF
                     RWSWTDAMVIGLVTLVARSAWHAVDRRPAINLAWEWLSLGLTYLLMRNLPRTRNESSA
                     LAGMFVATAFAVSCYGLYQVGVELPTIRAAFQRNPQAILRALDIEPGSRGEELLRNRL
                     LFSSELFSTFALANSLAGFVVGPFVLALAVSLQNLVRKDVPGSRWTALGMATPVILVL
                     LVGLILTKSRSAWVGLVVAMIFWAWHARRDLSVRVLLAAGLGGMVLVTALVAAGLATG
                     RLDREVLTQSTMSLRYRWEYWQGTWGVITGGASNLGSILRSPYLWWGVGPGNFSGHYL
                     KYKLPQASEEIQDPHNLFLEVWSTAGVWALLALIGALAWGLWNLLSSSRRVEERQGAS
                     PTDRGRNRDSRRESAVDMPRGRDRDQEIDRPPQRTTWLVGFAGVGGWVLVVLLGQLNP
                     FELQMSERWLILGMSWLAAVILGAPLWRRLPISAGALGAAVVAVVINLLAAGGIGVPT
                     VALLLWSMMALGLNLRDDRSCGRLREYESRVPPFVLAVAWAALFGTFAGLIAPFWRSE
                     AAIARAHDAVSHHPPNVEHADLEYQAAIAADRYSARAWRELANLHLRVWQDGGGAVDD
                     KETRWSMKTIPLLYQWAVTPPRNPDSWSMHNERARAIHQMLNVAGSKLQPLELMRLRG
                     ELVKSTRTASLLYPTNIELHARLAEASADISMYQDAVDEATEALRLDRIMPHRDKKLP
                     ASIRGHLEAQVPTWSENAARMPIQKTP"
     gene            complement(4505..>5344)
                     /locus_tag="VHS97_23025"
     CDS             complement(4505..>5344)
                     /locus_tag="VHS97_23025"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX3451146.1"
                     /translation="DIYNLNWKVRLVIQLTCAALVAFSGIRVTLFGPFTHPILGGTVT
                     VLWIVGLTNAFNMLDNMDGLSASVGLIAASLFCGAQLAAGSLFAPAVLLVLVGALAGF
                     LMHNHAPARLFMGDSGSNFLGFLLGALTVVGTFTRDERGGAYSPYGVLAPLLVMAVPL
                     YDMISVILIRLSEGRSPFVGDRRHFSHRLVARGLTASQAVLTIDLVTLAGGLGALLLH
                     RLGGLGACVVMAQTCCLLGVVGILELSASRSEQVNGQTRHEITGSAPVETSREAGAHA
                     GVG"
BASE COUNT         1080 a         1756 c         1608 g          900 t
ORIGIN      
        1 tcagtttcac caggtcgtac tcaatgatct agctccgacg ccacccgcgt ccggcctgct
       61 cgtcgtcgat ctggatgccg acggtcgggc tgacctggcg gctcccagcg cggaagggca
      121 cgtctatgcc tggcgaaacg cgacggaacg aaccacgacg acacagacca ggttgacttt
      181 tgagccctgg ccgaccaacg cgagccggtg gcgctcggct caggcgatcg acctcgatct
      241 cgacggccgg actgacctgc tgggtctgct tgcctcgtcg agcaaagtca gcgaattcga
      301 cgtgccactc tgggcccgaa acgaacgggc gctgctcgcg accaaaaccc ttcctgttca
      361 gctcgaaagc ccgggtctcg atggcttgat cgcggtcgac ctggtcggtg atccgcttcc
      421 cgatatcctt gccgtacgcc ccggtagtcc ccccgtattg gctcgcaacc tgggcaacgg
      481 caggcactgg ctggcgttcg agctcggtgg gctctggcac gtcaagccgg aactgatgag
      541 aaccaactct cacggactcg gtacccgcgt ccttgtcgaa gggcagggga cccatgttac
      601 gcatgagcat acaacacagg aaaccggctt ggggcaatcg gtcgtcccgt tcgtgcttgg
      661 cttgggcgag cgtgagcgag ccgagttgat ccatttgcgc tggcccgacg gcgtgatgca
      721 gtgcgagttg aacgttacgg gcgatcagaa gctgagcctg gtggaaaaga accgcaagac
      781 cggcagttgc ccggtccttt tcacgtggaa cggccggcgg ttcgtctgta tcggcgactt
      841 tctgggtgga gggggccttg gctaccttgt tgccccggga gtctacggcc agcccgaccg
      901 ggacgaggcg attgcggtta cggccgaaca actccaaccc agcaatgggg tcttgcgcat
      961 ttcgattacc gagccgatgg acgaggtcgc gtatctcgat catctgagac tcgacgccat
     1021 cgattgcccg ccgggcgtct ctgccacacc tgacgagcga ttcgcaccgg acggaacaag
     1081 accgaccggc gaattgatcg cctggcgggc ggccatcgaa cccgtgcgcg ccaccgattt
     1141 ggaaggccga gatctgacgg agatccttcg ctactgggat cgccgcacgg cggacttgtt
     1201 tcgcaagcgc gacggatgga tcggctacag cgaagaacat gcgatcattc tcgatttcgg
     1261 tgaccggctc agccgcttcg gcccgtccga tcgtctcgta ctctgtctgg caggctgggt
     1321 cgagtacccc tactcccaga ccaactacgc ggcggcgaca gccggcgtcg caattcttcc
     1381 tccggcaatc gagcgccgac gcgaggatgg tcgatttgaa accatcgagc ggaacgccgg
     1441 gtacccggcc ggcatgcccc ggctcatgac ggtcgacctg accggcaagc tcaccggtcc
     1501 caagtgcgtc cttcggatca agaccaatat ggagtgctat ttcgatcagg cgttcatcgt
     1561 cgccagggat cggttcgccg aatcgtcgct gcgtgtctcg agtctgcccg tggctcgggc
     1621 cgagctcggt catcgtggct atactcgcga aatatcgccg gacggacgac aaccgttgat
     1681 atacgattac gattatgttg atcccgctcc gctggcgagt ttcgcgggca aactcacccg
     1741 ctatggcgat gtggcgccat tgcttcggtc tgatgatgac cagctttgtc tggtcgggcc
     1801 gggcgatgaa gtgagaatcg agttccaggc gtcgactctg ccgccattgg caccggggtg
     1861 gacacgccgc tatgtggtcc ggagcttcgg atattgcaag gacgccgacc cgttcacggc
     1921 caccagcgat acgatcgagc ccttgccctg gcggacgatg ccagcctttc catttggtgc
     1981 cggcgtcaag cgacccagcg atccggctta tgagacgtat ctgcgcacat accagattcg
     2041 acccgccggg ggcggcaatt aggcggcgtt tggactcgac agactggctg gaatcgaaag
     2101 ccttgggttt catggcgtct tctggatcgg catcctggct gcgttttcgc tccacgtggg
     2161 gacctgggct tcgagatggc cccggatcga agcgggcagc ttcttgtcgc gatgaggcat
     2221 gatccggtcg agtcgcagag cttcggttgc ctcatcgacc gcgtcttgat acatgctgat
     2281 atcggcgctc gcttccgcca accgcgcatg gagctcgatg ttggtcggat agagcaacga
     2341 cgccgtccgc gttgatttga ccagctcacc ccgtaaacgc atgagttcca gcggctgcag
     2401 tttcgagccg gcgacattga gcatctggtg gatcgcccgc gctcgttcat tgtgcatgct
     2461 ccaggaatcg gggttgcgag gcggagtcac ggcccactgg tacaggagcg gaatagtctt
     2521 catgctccac cgcgtttcct tgtcatcgac ggcgccgcct ccatcttgcc acacccgaag
     2581 atgcaagttc gccagctccc gccaggcacg agcggagtac cgatcggccg cgatggcggc
     2641 ctggtactcc aggtcggcat gctcgacatt ggggggatga tgactcaccg cgtcgtgggc
     2701 tcgggcaatt gcggcttccg accgccagaa tggtgcgatc aaaccggcaa acgttccgaa
     2761 gagcgcggcc caggccacgg caaggacaaa aggaggaacg cgactctcgt actcacgaag
     2821 acgcccgcac gaccggtcgt cgcgcagatt gagcccgagt gccatcatcg accagagcaa
     2881 tagcgccaca gtggggacgc cgattccccc ggcggccagc aggttgatca cgaccgcgac
     2941 gaccgccgcc cctaacgcgc cggccgagat cgggagccgt cgccataagg gggcgcccag
     3001 gatcaccgcc gccagccagc tcatgcccaa gatcagccaa cgttccgaca tttgcaactc
     3061 gaacggattc aactggccca agagcaccac aagcacccag ccgccgaccc cggcgaaacc
     3121 aaccagccag gtcgtgcgtt gcggcggacg gtcgatctct tgatctcgat cccgccccct
     3181 cggcatgtcg accgccgatt cgcgccgtga gtcgcgattt cgaccgcgat cggtggggct
     3241 ggcgccctgg cgctcttcca cgcgccggga cgaacttaag agattccaca gcccccatgc
     3301 cagggcaccg atcagggcaa gcagagccca cacgcctgcc gttgaccaaa cttcgaggaa
     3361 caggttgtgc ggatcttgga tctcctcgct ggcttgtggc agcttgtact tgaggtagtg
     3421 accactgaag ttgcctggcc ccacccccca ccagaggtag ggcgacctca gaatgcttcc
     3481 caggttgctc gctcctcccg tgatgacccc ccaagtacct tgccagtatt cccagcggta
     3541 acgaagcgac atcgtcgact gcgtcagcac ttcacgatcg aggcggccgg tggcgagccc
     3601 cgccgccacc agtgccgtga ccagcaccat cccgcccaag cccgcagcca acagcacacg
     3661 aaccgaaaga tcccggcgcg cgtgccaggc ccagaagatc atggcgacca cgagccccac
     3721 ccacgcgctg cggctcttgg tgaggatcag gccgaccagc agaacgagaa tcacaggtgt
     3781 cgccatgccc agagcggtcc atctcgatcc cggcacgtcc ttgcgcacca ggttttgaag
     3841 actcacagcg agtgcgagca cgaatggccc cacgacgaag ccggccagcg agttggccag
     3901 ggcaaaggta gagaagagct cactggaaaa cagcagccgg ttcctcagca gctcctcacc
     3961 gcgcgagccc ggctcgatat cgagcgcacg caatattgct tgtgggttac gctgaaacgc
     4021 cgctcggatg gtgggcagct cgacaccaac ctgatagaga ccataacaag agacggcgaa
     4081 agcggtcgcc acaaacatgc cggccagggc cgacgactcg ttgcgcgttc gcggtaagtt
     4141 gcgcatgagc agatatgtga gaccaagcga gagccactcc catgccagat tgatcgccgg
     4201 ccgtcgatcg accgcgtgcc atgcgctgcg tgcaactaag gtcacgagcc cgatgaccat
     4261 ggcatcggtc cacgaccagc ggaagcgaaa gcggccgccg atgagcgcag cggccagggc
     4321 cagcccgaag acgacaaaga ccgagagcac ccacagcagc ccgcgcccgg ccccttcctt
     4381 caagtcgggc tcgctgggcc agaaagcgcg ggccgtcacg agcgcggccg tgagccccag
     4441 cgccagccgg cgaagattct cgcccagtcg cgtcgcctct tccgcgcggt cccggccgtc
     4501 atcttcagcc gacccccgcg tgggcgccgg cctctctgct ggtctcgacc ggggcggaac
     4561 cggtgatttc gtgcctcgtc tggccattta cttgctccga acgactcgcc gagagctcaa
     4621 gaatccccac gacccccagc aagcaacagg tctgggccat caccacgcaa gccccgagcc
     4681 cgcccaaccg gtgcaacagc aacgcgccta aacctcccgc caaggtgacc agatcgatcg
     4741 tcaaaacggc ctgagatgcc gtcagaccgc gcgccaccaa ccggtgcgag aaatgccggc
     4801 gatcaccgac aaacggactt cgaccctcgc tcaaacgaat caaaatgacg gatatcatgt
     4861 catataaggg aaccgccatg acaaggagcg gcgcgagcac gccataggga gaataggcac
     4921 cgccgcgctc atcgcgcgtg aacgtcccca cgaccgtgag tgcccccagc aagaacccga
     4981 ggaaattgct tcccgaatca cccatgaaga gccgagcggg cgcatggttg tgcatcaaaa
     5041 agcccgccag agcacccacc agaaccagca agaccgccgg cgcgaacaag ctgcccgccg
     5101 cgagctgggc gccgcagaac aaagatgcgg caatcagccc cacgctcgcc gagagcccgt
     5161 ccatgttgtc gagcatgttg aacgcattag tcaaacccac gatccacagc accgtcaccg
     5221 tgcctcccaa gataggatgg gtgaacgggc cgaacagagt gacgcgtatt ccactaaagg
     5281 cgaccagcgc ggcacaggtc aactggatga ccagccggac cttccaattg agattgtaga
     5341 tatc
//
LOCUS       DATAQL010001338         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1661623, whole genome shotgun sequence.
ACCESSION   DATAQL010001338 DATAQL010000000
VERSION     DATAQL010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661623"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="VHS97_23030"
     CDS             complement(<1..1511)
                     /locus_tag="VHS97_23030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451147.1"
                     /translation="MASLEKPHSPIETSTRFITSTHGAASLIKSAFHVLIRKGEQLVV
                     DGGVVRLSTKFVAITRREGLSVALAHATRKVRKKLSRFPLLSRLINPFSGCAYYTPPL
                     KMETYEVWRRLNRGNSLRRQLLGNARYPGGDMPRFSVVVPVYNPPVYVLRAMIASVFE
                     QSYSSWELILVDDASPDERVHRELQEWAERDRRINTIFRPDNGNISVATNQAAETARG
                     EFIVFVDHDDLLDHDALAHLALFLDAHPETDLVYSDDDKIGSDGIHHSPQFKPGWSPE
                     LLLSFCYTAHLSAVSSSLYRAVGGLRPGFEGSQDHDFWLRASELAKQVGHIPQVLYHW
                     RVLPGSTALDGACKPSSFEAGRRAVEEAFDRRGVTCRVEQADWAGRAGCAIFEPVMPD
                     DGPSVAILIPTRNHEYRLKKLLDSLAATTYRNYQLYIIDNASDEAGTLRYLASLPHKV
                     MRVPNPAGTFNFAAINNTAAAAVTEHLLLFLNDDVEVSNPRWLSQMVGWSRLPG"
BASE COUNT          349 a          465 c          505 g          342 t
ORIGIN      
        1 cccggcagcc gcgaccaacc caccatctgg ctcagccatc gcggattcga gacctcgaca
       61 tcgtcattga ggaataagag caggtgttcg gtgactgccg cggccgctgt gttgttgatc
      121 gcggcgaaat tgaatgttcc cgcgggattg gggacgcgca tgaccttgtg tggtaacgac
      181 gccagatagc ggagcgtccc agcttcatca ctggcgttat caataatata gagttgatag
      241 tttcgataag ttgttgcggc taacgagtcc agcaatttct tcaggcgata ttcatggttc
      301 cgtgtgggta tcagaatcgc gacggatggg ccgtcgtcgg gcatgaccgg ttcaaaaata
      361 gcacaaccgg cgcgtccggc ccaatcggcc tgttcgactc gacaagtaac tccgcgccgg
      421 tcaaaagcct cttcgacagc ccggcggccc gcctcgaagc tgctcggttt gcacgcacca
      481 tcgagcgcgg tcgatccggg caagactcgc cagtgataga ggacttgtgg gatgtggccg
      541 acttgctttg ccagctcact cgcgcgcagc cagaagtcat gatcttggga cccttcaaag
      601 ccggggcgaa gcccgcctac tgcacgatag agactgctcg ataccgccga cagatgggca
      661 gtgtaacaga acgataaaag gagctcgggc gaccaaccgg gcttgaactg aggggaatgg
      721 tggatgccat cggatccgat cttgtcatcg tcgctataga ccaggtcggt ttccggatgg
      781 gcatcgagaa agagtgccag gtgggccaat gcgtcgtgat cgaggagatc gtcatggtcg
      841 acaaagacga tgaattcgcc gcgcgcggtc tcggctgctt ggttggtcgc gaccgagatg
      901 ttgccgttat cgggccggaa aattgtattg attcgtcggt cgcgctcggc ccattcttgc
      961 agctcccggt gcacgcgctc gtcagggctg gcatcatcga ccagaatgag ctcccacgaa
     1021 gaatagctct gctcgaacac gctggcgatc atcgcccgta acacatagac gggtggatta
     1081 tagaccggca cgacgaccga gaaccgcggc atgtcgccac ctggatagcg agcgttcccg
     1141 agcagctggc gtcgcaatga attgccgcga ttgagcctgc gccagacctc gtacgtctcc
     1201 attttcaggg gcggagtgta atacgcacaa ccgctgaatg gattgatcaa acgtgagagc
     1261 agaggaaacc ggcttagttt cttccggacc ttacgggttg catgggccaa tgcgacagac
     1321 agcccttcgc gacgtgtgat cgcgacaaat ttcgtgctca agcgtacgac cccgccgtcg
     1381 acgacgagct gctcgccttt tcggatcaag acgtgaaacg ctgacttgat caagctggcg
     1441 gcgccgtggg tcgatgttat gaaacgtgtc gaagtctcga tgggggaatg cggcttttcg
     1501 agagaggcca tgttcgctcg atccttcgag tgcacgttcg ggccgaattc cgcgtcggct
     1561 ttccccccct tgtgacgggg gggttaggag gggtggtccc ggcgctatca gccgcgaggc
     1621 ttcaaaaaaa caacccaata ccgcgtcagc tttccccccc t
//
LOCUS       DATAQL010001339         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1968995, whole genome shotgun sequence.
ACCESSION   DATAQL010001339 DATAQL010000000
VERSION     DATAQL010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968995"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2466
                     /locus_tag="VHS97_23035"
     CDS             <1..2466
                     /locus_tag="VHS97_23035"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3451148.1"
                     /translation="PGAHERSPIWSPDGKSIAFFSDASGEYALVVCPQDGKGEVRSYP
                     LGGAGFYERPVWSPDSQKIAYIDNARALYWIDLGTGAQKWIDAEPVYSPINTLSYRWS
                     PDSKWLAYTHSSRAGFQSIQLYSLATTKSHALTDSLIEAGEPAFDSGGKYLYFLGSTD
                     AGPVKNWFDQSNTDMQATSSVYLMTLAKSTPNPLLKESDEEPAAVTKDGPKTQEPPVT
                     KLKDTRRLDNVTDSVQIDRIIKENEKILEENRKIVEEDQKKLKDDLEKTKKESETNAV
                     VAIDFDGIAGRVVALPIESGHISNLSAGTDGQIYFIRRVGAIPGRGPAARGKASLRRF
                     DWKTREEETLAENIDEFHLSADHKKILYRVSAPPRMSPFSEPPAPPVMGIVDAGKFTK
                     GDGTLDLESLSVPIEPRAEWAQILGEAWRINRDYFYAPNMHGADWKRIRAKYEELLPH
                     LTNRGDLNDVMRMMLSELSVGHSYLGGGERLYEPKPIPVGLLGADYEAAEGRDRFKTI
                     YGGAYWDPSLRAPLTAPGVDVKTGEFLMAVDGRELKTDSEVYRLFEKTVGKRIELKVG
                     PRSDGSGARTVVVEPIADESGLRNRAWVEGNLRKVHERTGGKVAYVYVPNTAEVGFTY
                     FKRYFYPQADKEAIIVDERFNGGGQVADYYIDMLRRPLVSFWATRHGEPIRTPSAAIM
                     GPKVMIIDETAGSGGDLLPWMFRKFRLGTLVGKRTWGGLVGILGFPALMDGGAVTAPD
                     LAIFTEEGWVVENVGVAPDIEVEQDPAAVAAGKDPQLDRAIEVVLAELAKSPPVRPPA
                     RPPFPVRVRTGANGNSVSAGTAR"
     gene            complement(2496..2948)
                     /locus_tag="VHS97_23040"
     CDS             complement(2496..2948)
                     /locus_tag="VHS97_23040"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX3451149.1"
                     /translation="MQKTHETLIMGGLLAMGAVALAASLSVGSTKADQGPQLAHMVYF
                     KLKDNSGAYRAKLSAACKLYLSGHEGTVSFGTGTRAGDFNRDVNDRDFDVSLHLVFVN
                     KEAHDKYHVHPRHLKFIEENKDNWEKVRVFDSYLSPALPSTRTTSIEK"
BASE COUNT          679 a          893 c          952 g          605 t
ORIGIN      
        1 cccggcgcac acgagcgttc gccgatatgg tcgccggacg gcaagtcgat cgcctttttt
       61 tccgacgcct ctggtgagta tgccctggtc gtctgtcctc aggatggtaa gggggaagtc
      121 cgctcttatc ccttgggcgg ggcaggcttt tatgaacgac cggtctggtc gcccgatagc
      181 cagaaaatcg cctatatcga caatgcccgc gcactctact ggatcgatct cggcaccggc
      241 gcccagaaat ggatcgatgc ggagccagtc tacagcccga tcaacacgct ctcgtatcgg
      301 tggtcacccg actcgaaatg gctggcctac acccattcga gccgagcggg atttcaatcg
      361 atccagctct attcgctcgc gaccacaaaa tcccacgcct tgaccgacag cctgatcgag
      421 gccggcgagc ccgcgttcga ctcgggcggc aagtacctct acttcctcgg gtcaaccgat
      481 gcgggacccg tcaaaaactg gtttgaccag tccaataccg acatgcaggc cacttcgtcg
      541 gtctatctga tgacgctcgc aaaatcgact cctaacccgc tgttgaaaga gagcgacgag
      601 gagccggctg ccgtgaccaa ggacggaccg aagactcagg aaccgccggt gacgaagctc
      661 aaggatactc gcagactgga taacgttacc gactcggtgc aaattgacag aatcataaaa
      721 gagaatgaga aaatattaga ggaaaacagg aaaatagttg aggaagatca aaagaagttg
      781 aaagatgatc tcgaaaaaac aaaaaaggag agcgagacca atgcggtcgt cgcgatcgac
      841 ttcgacggga tcgccggccg ggttgtcgcg ttgccgatcg aatcaggaca catcagcaat
      901 ttgtcggcgg gaaccgacgg gcagatctac ttcattcgca gggtcggagc cataccaggc
      961 cgggggccgg ccgcgcgcgg taaggcctca ctgagacggt ttgactggaa gacacgcgaa
     1021 gaggaaactc tggccgaaaa catcgacgag ttccatctct cggccgatca caagaagatt
     1081 ctttatcgtg tcagtgcgcc gccacggatg agcccgttca gcgagccgcc ggcaccgcct
     1141 gtcatgggca ttgtcgatgc aggtaagttc accaagggcg atggcacgct cgatctcgag
     1201 tcgttgtcgg taccgatcga gccgagggcg gagtgggcgc agatcttggg cgaggcctgg
     1261 cggatcaatc gcgattactt ttacgcgccc aacatgcacg gagccgactg gaagcgcatc
     1321 cgagccaagt acgaagagct cctcccccac ctgaccaatc gcggcgatct gaacgacgtg
     1381 atgcgcatga tgctcagcga gctgtcggtt ggccacagct atttgggggg tggagagcgg
     1441 ctctatgagc cgaaacccat acccgtcggc ttgctcggcg ccgactacga ggcggctgaa
     1501 ggccgcgacc gattcaagac gatctacggc ggagcgtact gggacccaag ccttcgtgcc
     1561 cccctgactg cgccgggagt cgacgtaaag acgggtgagt ttctcatggc cgtcgatggc
     1621 agggagttga agacagacag cgaggtctat cggctctttg agaaaaccgt cggcaaacga
     1681 attgagctca aggtgggtcc ccgttccgat gggtcgggtg cgcggacggt ggtcgtcgag
     1741 cccattgccg atgagagcgg tctgcgcaat cgcgcatggg tcgaaggtaa cctccgcaag
     1801 gtccacgaac ggaccggtgg aaaagtggcc tacgtctatg tgcctaacac ggccgaagtt
     1861 ggctttacct acttcaagcg ctatttttat ccccaagccg acaaagaggc gatcatcgtc
     1921 gatgaacgct ttaatggcgg gggccaggtt gccgactatt acatcgatat gctccgtcgg
     1981 ccgctggtca gcttctgggc aacccggcac ggcgagccaa ttcgcacccc gagtgccgcc
     2041 atcatggggc ccaaggtgat gatcatcgac gagacggccg gttcgggagg tgacctcttg
     2101 ccgtggatgt ttcgtaagtt ccgactcgga accttggtcg gcaagaggac ctggggcggc
     2161 ctggtcggta tacttggctt tcccgcccta atggatggcg gcgccgtcac ggcgcccgac
     2221 ctggccatct ttaccgagga gggttgggtc gttgaaaacg tcggggtcgc tcctgacatc
     2281 gaggtcgagc aagacccggc cgccgtggct gccggcaaag acccgcagct tgaccgagcc
     2341 atcgaggttg tcctggccga actggccaag agccccccgg tccggccgcc ggcgcggccg
     2401 ccatttcctg ttcgcgtgcg gaccggcgca aacggcaatt ccgtgagcgc cggcaccgcg
     2461 cggtgaagtc aaggaattcg cgtcctcggc attcgtcatt tctcgatgga cgtggtcctg
     2521 gtcgaaggga gagctggcga gagatacgaa tcaaagaccc ggaccttctc ccagttatcc
     2581 ttgttttcct cgatgaactt taagtgccga ggatgcacgt ggtatttgtc gtgagcctct
     2641 ttgttgacga agacaagatg gagggagaca tcgaagtcgc gatcgttgac gtcgcggttg
     2701 aagtcgcctg cgcgggtacc cgtgccgaac gagacggtcc cttcatgacc cgagagatag
     2761 agcttgcacg cggcagacag tttggcccgg taagctcctg aattatcctt gagcttgaaa
     2821 tacaccatgt gagccagctg aggcccttgg tccgccttgg tcgacccgac ggagagtgag
     2881 gctgccagtg ctacggcacc catggccaat aagccaccca tgatcagcgt ttcgtgtgtc
     2941 ttttgcatga gacttcctca aaaataagaa tgctcgaatc gacgtgcgaa ccgacggcgc
     3001 gacgtcatat cgtggtccgt catgatcgcc cgaagtcatt aggatcgcaa gtctccaccc
     3061 ggtgttccgg gctcgatcaa atcacggtca atttgccggg gattctttcc gcagcagcgg
     3121 taggggcac
//
LOCUS       DATAQL010001340         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1357758, whole genome shotgun sequence.
ACCESSION   DATAQL010001340 DATAQL010000000
VERSION     DATAQL010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357758"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VHS97_23045"
     CDS             <1..295
                     /locus_tag="VHS97_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3451150.1"
                     /translation="MDVAGGHAAARQTGLAILATMRAHLGSLDKVVRLIKSLGMVNAT
                     PEFTDHPKVINGYSDLMKEVFGEAGVGTRSAVGMGGLPGGIAVEIEAIFQVKD"
     gene            339..1193
                     /locus_tag="VHS97_23050"
     CDS             339..1193
                     /locus_tag="VHS97_23050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3451151.1"
                     /translation="MLRPGRLVGRTCLIVGGTSGIGLASARRFLEEGARIVVTGRDPE
                     IGRTPLEQLAPFGSASEFSLELDQGEEGIARMFAFALDALGGRLDVLLHVAGISGRKF
                     GDGPLHDCSTEGWERVMRTNAEGAFLTNRAAVRLMLDQPIDANGLRGTVVNVGSVLDR
                     SPSPVHFGTIAYAASKGAIRSMTLSAAARYAADHIRFNLLVPGLIDTPMAARATNDSR
                     IRNYLATKQPIAAGPGSADDVADAALYLCEPASRFVTGAELVVDGGWSVSEGLEEGEA
                     PSEPNHSS"
     gene            1190..1972
                     /locus_tag="VHS97_23055"
     CDS             1190..1972
                     /locus_tag="VHS97_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3451152.1"
                     /translation="MSDRPDVFSQYLAAVRAAVDDIEATQLPLIREAAARFAETIKKE
                     RLVHVFGTGHSRMAVEEMFPRYGSFPGFHPIVELSMTYHNAVVGANGQRQAMFLENVQ
                     GFGAVLWRNFATAPDDALLAISTSGCNAVTIDVALEAKKRGMVVVALTSLDHAAVSAS
                     RHESGKKLHEVADLVLDQKAPAGDSAVWIDGLETPVAPLSSVTGCTIINLVKAEVARL
                     LTEAGSPPKVLTAACHLGDARARELFEETYDEYRRRVGVLYR"
BASE COUNT          391 a          643 c          694 g          431 t
ORIGIN      
        1 catggacgtg gcgggcggtc acgcggcggc gcggcagacc gggctcgcca ttctggcgac
       61 gatgcgagcc catctcggat cactcgacaa ggtcgtgcgg ctgatcaaga gcttgggtat
      121 ggtgaacgcg acccctgaat tcacggacca ccccaaggtc atcaacggct attccgattt
      181 gatgaaagag gtttttggcg aagcaggggt tggaacccga agcgctgtcg gaatgggggg
      241 gcttcccgga ggaatcgctg tcgagattga ggcgattttc caggtcaagg attgagtcgt
      301 tcacttggtg agcaccgttg ccgatggagg tctcgcgcgt gctacgacct ggccggctcg
      361 ttgggcggac gtgcttgatt gtcgggggga cgagcggtat cggcctggcc tcagcacgac
      421 ggttcctaga ggaaggggct cggatcgtcg tgaccgggag agaccctgag atcggccgca
      481 cgccgctgga acaactcgct ccattcggct ctgcgtcgga gttctcgctc gagctcgacc
      541 agggtgaaga gggcattgcg cggatgttcg cgttcgcact tgacgcgctg ggcggacggc
      601 tcgatgtcct tttgcacgtc gccgggatca gcggtcggaa gtttggcgat gggccgttgc
      661 acgattgttc gaccgaaggt tgggagcgcg tgatgcggac caacgcagag ggtgcgtttt
      721 tgaccaatcg cgcagccgtg cggctcatgc tcgatcagcc gattgacgcc aacggcctgc
      781 gaggaaccgt ggtgaatgtc ggttcggtcc tggaccgttc gccctcgccg gtgcatttcg
      841 gtacgatcgc ttacgcggcc agcaagggag ccatacgttc gatgactttg tccgccgcgg
      901 cgcggtatgc cgctgaccat atccggttca acttgctcgt accgggtttg atcgacacgc
      961 cgatggcagc gcgagccacg aacgattccc gaatccggaa ctacctggcg accaagcaac
     1021 cgatcgccgc tggtcccgga tcggctgacg acgtcgccga tgccgcactc tatctctgcg
     1081 agcccgcgtc acggttcgtt acgggtgccg aactggttgt cgatggtggc tggtctgtat
     1141 ctgaaggtct tgaagagggc gaggctccat ccgagccgaa tcattcctca tgagcgatcg
     1201 ccccgacgtg ttctcccagt atctggccgc cgtccgcgcc gcagtcgacg acattgaggc
     1261 gacgcaattg cccctgatcc gcgaggcggc cgctcgcttc gcggagacga tcaagaagga
     1321 acgcttggtc catgtatttg gcaccggcca ttcgcgaatg gcggtcgaag agatgtttcc
     1381 ccgctatgga tcctttccgg gctttcatcc tatcgtcgaa ctttcgatga cgtatcacaa
     1441 cgccgtggtg ggagccaatg gtcagcgcca ggcgatgttc ctggaaaacg tgcaaggctt
     1501 tggtgcggtc ctgtggcgta atttcgcgac cgcgcccgac gacgcgctct tggcgatctc
     1561 gaccagtggt tgtaacgccg tgaccattga cgtcgccctg gaggccaaga agcgcggtat
     1621 ggtcgtcgtt gctctgacat ccctggacca cgccgccgtc tcggcttcgc gacacgaatc
     1681 gggaaaaaag ctgcatgagg tcgctgattt ggtgctcgac cagaaagccc cggccggcga
     1741 ctcggccgtc tggatcgacg gcttggagac tccggtcgcg cctctttcga gcgtcaccgg
     1801 ctgcacgatc atcaacctgg tcaaagccga ggttgctcgc ctgctcaccg aggctggatc
     1861 gccacccaag gtgttaacag ccgcctgtca tctgggtgac gcccgggcgc gcgagctttt
     1921 cgaggaaacg tacgacgaat accgccgccg cgtaggtgtg ctctatcggt agaaccagtt
     1981 ccgacgcgct accccgcaca accgtagcgc ccatagcaaa cggagaacgc gctggttttc
     2041 caggccgaac aaacacgacg gcttccccaa tcccgaacga tcgtcaggca tcctcgtgat
     2101 cccgaaagaa cgcgatgagc gataccttgc gttcggccgc gatggagtcc gtcagacgc
//
LOCUS       DATAQL010001341         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4869584, whole genome shotgun sequence.
ACCESSION   DATAQL010001341 DATAQL010000000
VERSION     DATAQL010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4869584"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            783..1349
                     /locus_tag="VHS97_23060"
     CDS             783..1349
                     /locus_tag="VHS97_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3451153.1"
                     /translation="MTEVTHILRHSEAGEAERLLPLVYGELKKLSAAWLAHELPGQTL
                     QPTPLVHEAYVRLVDVQQAQGWRSRGHFFAAAAEAMRRILVDAARRKRRDKHGGNRQR
                     VSLDDIAPTAPDFRHDLLALDAALTQLTDQDPQAARLVELRHFAGLSLSEASQVLGIS
                     PRTADRVWAYARAWLHREITAEVDPADS"
     gene            1458..2036
                     /locus_tag="VHS97_23065"
     CDS             1458..2036
                     /locus_tag="VHS97_23065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451154.1"
                     /translation="MSERELFLATLELPDPRARALYLDKACAGDSALRARVEALLKSH
                     DEAGSFLNKPAMGTTIPPAAPGASHDMSTTGPGPALQATRAVIGEAQARADLSVAARA
                     DATGGWTTDPNDSARTADGNGASRDLTRGATVRYFGDYEIQKELGRGGMGVVYKARQL
                     SLNRPVALKMIKAGLLADDAELRRFQNEAWLR"
     gene            2041..2610
                     /locus_tag="VHS97_23070"
     CDS             2041..2610
                     /locus_tag="VHS97_23070"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX3451155.1"
                     /translation="MRRADQFVLIGSDVPLSWLVMMAVHEAGHVLGALVTGGTVKVVV
                     LHPLKISCTVLSHNPHPLLVTWAGPVIGVLIPLAAFGVAAAIRMPGAHLLRFFAGFCL
                     IANGAYIGVGSIERIYDADDLLRHGSQTRHLWFFGAVTIPLGLLLWHRLGPYFGLGKA
                     RGQVSRGAAIACLVLLVVVAGLEFVLGDE"
     gene            2977..4053
                     /locus_tag="VHS97_23075"
     CDS             2977..4053
                     /locus_tag="VHS97_23075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX3451156.1"
                     /translation="MAKSLPPRPNLDHLRRQAKALMSAVQTGDADAVAMIRDHLPAAK
                     GMTAEQVCQAGFRLADAQSAVARKTGFASWPQLARHVEQLRALEGTWVFAHLEVDGHA
                     VPPGMMGNSRILIDGDCFRTESPEATYEGVFNINVEARPHEIDIEFIAWPEAGNWNFG
                     IFRLAGDQFELCLDLNGQPRPTEFCTSPRSGHAYERLTRSSGARPANVSGGTPPAQQA
                     LPTKVDSTEFSYVECPTLMRLQGEWAAVQVVLDGNELPPMMLATGLRTATQNQVKVTF
                     GGQLMVDALARIDERADPIHIDYFNRCGQAKGQVQLGIIQWVGQEACIIMAAPGQARP
                     TDFTCPAGSGRTLSRWRRRTSRGS"
     gene            4236..4487
                     /locus_tag="VHS97_23080"
     CDS             4236..4487
                     /locus_tag="VHS97_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451157.1"
                     /translation="MGASVDQTEARCARLLKAAELTGLRAQADGEGGVADERLEQPRL
                     LVQAAQLFDQGRPQLHSRQAIGSTRAEVLDSFSVDHEDP"
     gene            4484..>4711
                     /locus_tag="VHS97_23085"
     CDS             4484..>4711
                     /locus_tag="VHS97_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451158.1"
                     /translation="MTAGLAGCTDDEDKQHSAARDRMRRVKNWQSGVMALWSTAAAFD
                     SASKEFRRIMDHEHFWMLKAALDEHSCDQSLV"
BASE COUNT          906 a         1444 c         1478 g          883 t
ORIGIN      
        1 gactcggcac gcacaaccgc cgcgggatcc tgacggtggg tggttccgtc aaggacgaca
       61 tcgatcacgt ggaccaccac ctcgcgttcc tcgtcgaaaa gagagcccgc ctcgagaagc
      121 tccgacccca gccaccccaa cccgcgcctc atgcgcaacg caagggtgag gtgccgtagg
      181 ctccgaaacg ccgatccgag gcgcttcggt ccgacacacc aaagcatcga acatgcgatt
      241 tgcgcgcgtc ggtcgatcag acgaatggca ctagcttagg cgtaagtcat ctcaggtaaa
      301 gcatttggac tcgtagcagg cggatttctc gcaggtaatt tcataaattg ttcaaattac
      361 tactattgga ctagatgaga aacaaggggc ttcagatgga gtccccaaga atcaagccgg
      421 agaagcagga aacgcaattt ctgtaagttt aattccgata atggtttgcg cttaagctag
      481 tgccattcgt cgatcagacc gacgcctcaa gacccgcggg gccatcttgc ggcccgagaa
      541 ccgcaaggac tgcggcatcg ccagcggctc ggcgaggcag cggtttccag cgacccgcca
      601 ccccgggacc attttcaaga cgatgattgg acgccgtaga cggacgatcg aggccgggag
      661 tcacgacccg gcgaacggcc gttccggatc acctcacgtg gttcgccgat ctcgaacccg
      721 tacgcattcg ggaacccgca cggattcgcg ttccagagca acagtggttt acgctacttg
      781 acatgaccga agtcacccat atccttcgac acagcgaggc aggcgaggca gagagactgc
      841 tgcctttggt gtacggtgaa ctgaagaaac tctcggcggc ttggttggcc cacgagcttc
      901 cggggcaaac cctgcagcct acccctctcg tccacgaggc ctacgtgcgg ttggtcgatg
      961 tccagcaggc gcagggctgg cgaagccgag gtcacttctt cgccgcggcc gccgaggcga
     1021 tgaggcgcat tctggtcgac gcagcccggc ggaaacgacg agacaaacat ggggggaatc
     1081 ggcaacgcgt ctctctcgac gatatcgccc ccacggctcc ggatttccgg catgacctgc
     1141 tcgcgctgga cgcggctctc actcagttga ccgaccagga cccgcaagcg gcccggctgg
     1201 ttgaacttcg ccacttcgcc ggtctgtccc tgtctgaggc gtcgcaagtg cttggcatct
     1261 caccacgtac ggccgatcgc gtgtgggcct atgcccgggc ctggctgcac cgggaaatca
     1321 ccgccgaggt cgatccggcg gattcctgaa aagtaattgg cgtgattccc gcaagaactc
     1381 gcattggtag caggccagcc aagctgtccc ggctggcggg tcgtggtcga gcgcagttcc
     1441 aagaagggcc cccagtcatg agcgaacgcg aattgttcct ggcgacgctc gagcttccgg
     1501 atcctcgtgc gagggccctc tacctggaca aagcctgcgc gggcgattcc gcgctccgtg
     1561 ccagggtcga ggccctgctc aaatcgcacg acgaggccgg tagtttcctg aacaaacccg
     1621 caatgggcac cacgatccct ccggcggcgc ccggcgcctc tcacgacatg tccaccacgg
     1681 gccccggccc agcgctccag gcaacccgtg ctgtgattgg ggaagcgcaa gcgcgcgcgg
     1741 atttgtctgt cgcggcacgg gccgacgcca ccggcggctg gacaacggat cccaacgatt
     1801 cagctcgcac ggccgacggg aacggagcca gccgcgacct cacccgcggc gccaccgtcc
     1861 gctacttcgg cgactacgag attcagaaag agctggggcg cggcggcatg ggcgtggtct
     1921 acaaggcccg ccagctcagc ctcaatcgac ccgtcgccct caagatgatt aaggccggtc
     1981 tgctggccga cgatgccgag ctgcggcgat ttcagaacga ggcgtggttg aggtaagccc
     2041 atgcggcgag ctgaccagtt tgtcctcatc ggctcggacg ttccgctttc ttggctggtt
     2101 atgatggccg tccacgaagc tggccacgtt ctaggggccc tcgtgactgg cggcaccgtc
     2161 aaagtggttg tcctccatcc gctcaagatc tcctgcaccg tcctctccca caacccacat
     2221 cctctgctcg tcacctgggc tggtccggtg attggtgtcc ttattccgct cgcggccttt
     2281 ggcgtcgcgg ccgccataag aatgcccggt gcccacttgc tgcgtttctt cgccgggttc
     2341 tgcttgattg ccaacggggc ctacatcggt gttggttcca tcgagaggat ttacgatgcg
     2401 gacgatttgc tccgtcatgg cagtcagact cggcacctgt ggttctttgg ggccgtcact
     2461 atcccattgg gtttgttgct ttggcaccga ttggggccgt atttcggact tggcaaggct
     2521 agaggccagg tgagccgagg tgccgctatt gcctgcctgg ttctgctggt cgtcgttgct
     2581 gggttggagt tcgttctcgg tgatgagtaa agtcagatgt taccctgctg acgggcgacg
     2641 tggatgaagc ggattgggtg gtttatgatg acccgggtca gtcggggcga caggacaccg
     2701 ttagaacttt ttctgggggg catgactggg tgggaggcct ggatacggca gtggattgaa
     2761 ccgtctcaac cgcaaccgga gttgtgatga cggataaccc agttaagtta cttgcctgcg
     2821 tttccgaggg ttcgtatcat aacggcggct cgaagttagc cgccggatcc gcgggaaggg
     2881 tttaacccag cttcatctca ccaacaactt gtgatcgatc gcacgttctt cgtgccgcct
     2941 gcttgcggat cccggcgcga acatggagaa cgtcacatgg ccaagagcct tcctccccgt
     3001 cccaatctcg accacctccg gcgtcaagcg aaagcgctga tgagcgctgt gcagaccggc
     3061 gacgcggacg ccgtggcgat gatccgggat catctacccg cagccaaagg catgacagcc
     3121 gaacaggttt gtcaagcggg attccgattg gcggatgcgc agtccgccgt tgcccgcaaa
     3181 actggattcg ccagttggcc gcaactggcc cggcatgtgg agcagttgcg ggcgctcgaa
     3241 gggacgtggg tgttcgccca cttggaggtt gacggtcacg ccgtcccgcc gggcatgatg
     3301 ggcaactcac gcatcctcat cgacggtgac tgcttccgca ccgagtctcc ggaagcgacc
     3361 tacgaaggag ttttcaacat caacgtcgaa gcccggccgc acgagatcga catcgagttc
     3421 atcgcgtggc cggaggctgg gaactggaat tttggcatct tccgtcttgc cggcgaccaa
     3481 ttcgaactat gcctcgacct gaacggccaa ccgcgcccga ccgagttctg cacgtcgccg
     3541 cgcagcggcc acgcttacga gcggctgacg cgcagttccg gtgcacggcc ggcaaacgtg
     3601 agtggtggta cgccgccggc gcagcaagcg ctgccgacca aggttgatag cacggaattc
     3661 agttacgtcg agtgccccac gctgatgcgc ctgcaaggcg aatgggcggc tgtgcaagtc
     3721 gtcctcgacg gcaacgaact gccgccgatg atgcttgcca cgggcctccg tacagcgacg
     3781 caaaatcagg tgaaggtcac cttcggcggg cagctgatgg tcgatgccct ggcccgcatc
     3841 gacgagcgcg cggatccgat ccacatcgac tatttcaacc gctgcgggca ggccaagggg
     3901 caggtgcaac tgggcatcat acagtgggtc ggccaggagg cctgcatcat catggccgcg
     3961 cccggccagg cacggccgac cgactttaca tgcccagcag gcagcgggcg cacgctcagc
     4021 cggtggcggc ggaggaccag ccgggggagt tgaatcgggt cgccgaccag cgccgcttcc
     4081 catcctctct tctcaagggt tgcgtcgcga ttcgggaacc gtttctggat gtaatcgttt
     4141 gaattccgtg acgaccagtg ggcgaaagcg gttacgacaa ctgccgaaaa gaacaatggc
     4201 gacgcacgac gcaatttgaa ccatcgagca cgcgattggg cgcatccgtc gatcagaccg
     4261 aagcgcgctg tgccagattg ttgaaggcgg cggaactcac cggacttcgt gcgcaagcgg
     4321 acggggaagg cggcgtggcg gatgaacgac ttgagcaacc tcggctgctg gtgcaggcgg
     4381 cccagctctt cgaccaaggc cgccctcagc tccactctcg gcaggcgatc ggatcgacgc
     4441 gcgcggaagt gcttgactcg ttcagcgtcg atcatgagga tccgtgacgg cagggctagc
     4501 aggatgcacg gacgacgagg acaagcagca ctcggcagcc cgcgatcgta tgcgtcgggt
     4561 gaagaactgg caaagcggcg tgatggcgtt gtggtcgaca gcagcggcct tcgattcggc
     4621 ctcgaaggag ttccgccgga tcatggacca tgaacatttc tggatgctga aggccgcttt
     4681 ggacgagcac tcttgcgatc agtcgcttgt c
//
LOCUS       DATAQL010001342         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1512453, whole genome shotgun sequence.
ACCESSION   DATAQL010001342 DATAQL010000000
VERSION     DATAQL010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512453"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VHS97_23090"
     CDS             complement(<1..409)
                     /locus_tag="VHS97_23090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3451159.1"
                     /translation="MRRRRFLEGCGLGLTSLLAAQSGNARARAAGRGATEITADIVIV
                     GGGLGGCAAALAALRAGRSVILTESTDWIGGQLTSQAVPPDEHPWIEQFGASASYRTL
                     REGIRDFYRRRYPLTADAHAQRFFNPGSGGVSRL"
     gene            514..596
                     /locus_tag="VHS97_23095"
     tRNA            514..596
                     /locus_tag="VHS97_23095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:547..549,aa:Leu,seq:tag)
     gene            993..1325
                     /locus_tag="VHS97_23100"
     CDS             993..1325
                     /locus_tag="VHS97_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451160.1"
                     /translation="MIGLRELALVAVVVLVLYGRTGVVKSRQFQTILPWISPFKRPRG
                     KTGAMNSPRARSASATAEAMLPRPKPFLLRGNRLFWFLTILAATAVAAWIVTRVIIVS
                     TAGPGVTH"
     gene            1420..1632
                     /locus_tag="VHS97_23105"
     CDS             1420..1632
                     /locus_tag="VHS97_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX3451161.1"
                     /translation="MMPFAFLPTFGPWEMIIVGMVCLLIFGNRLPSVMRSLGKSVTEF
                     KKGISGIEDDLDQAVTADKKPTPPNA"
     gene            1763..2104
                     /locus_tag="VHS97_23110"
     CDS             1763..2104
                     /locus_tag="VHS97_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX3451162.1"
                     /translation="MFNLGPMEMLIVMGIAVLLFGKRLPEVGRSLGKGIVEFKKGLSG
                     VGDEFDISGTGTGRSSTGYGSSWRADDRPSGESSYADASVPKFELPGAASSAEPVSSP
                     HTEFQADQPRY"
     gene            complement(2129..2635)
                     /locus_tag="VHS97_23115"
     CDS             complement(2129..2635)
                     /locus_tag="VHS97_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX3451163.1"
                     /translation="MLTEEIPPRPSSFSRVSLAIITEPGQANAFGTLHGGVILKLADE
                     CGAIAALRHAGKGQITTAAIDSMIFLSPVYVGERVEFSAEVTYVGRTSIETKIEIFAE
                     PIEVSERRKVAVGYALYVALDESGRRPRPVPPLLIDNDAGHRAALAAQARQTNRLARR
                     ALAQGQGT"
     gene            2771..3310
                     /locus_tag="VHS97_23120"
     CDS             2771..3310
                     /locus_tag="VHS97_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451164.1"
                     /translation="MLIDQFNELIDPVIRAGGSVLEIGEEFREPPLDVLRYYHRPVSM
                     NWVPVLGKAHSVVALVQQPVDIDGSRAGQERVLTRLAMAVNGRFPPWRGLVIGLTALV
                     LTPEPIGPGDDAILRDVLEVKLKRMRVVPFAFLRVNLGQEAIALAIHSSPDSLFPEPM
                     LLGDALCERFRRYVPLIDM"
     gene            complement(3324..3476)
                     /locus_tag="VHS97_23125"
     CDS             complement(3324..3476)
                     /locus_tag="VHS97_23125"
                     /inference="COORDINATES: protein motif:HMM:NF022101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEX3451165.1"
                     /translation="MTYAIDPPSAGGLGRHVDPATGVDPWWKPFQVSFDWDYPGPPSA
                     PPSAVN"
BASE COUNT          639 a         1070 c         1092 g          692 t
ORIGIN      
        1 agagccgcga cacgccaccg ctcccgggat tgaaaaaccg ctgtgcgtgg gcgtcggcgg
       61 tgagggggta acgccggcgg taaaaatcgc gaataccctc tctgagagtc ctgtaggatg
      121 cactcgcgcc aaactgctcg atccaggggt gctcgtcggg gggcacggcc tgggaagtga
      181 gctgaccgcc gatccagtcg gtggattcag ttagaatgac cgaacggccg gctcgtaacg
      241 cggccagtgc cgcggcacag cctcccagcc cgcctcccac gatgacgatg tccgccgtaa
      301 tctcggtcgc gccccggccg gctgcgcgag cccgtgcatt ccctgattga gccgcgagca
      361 ggctggttaa ccccaagccg cacccctcga gaaatcgtcg gcgtcgcatg gtcgctgtga
      421 gtcccttggc aatgaaggcg tcaatgagga taattctgaa atcgtagaca tcgactcggc
      481 accgccgata gacagcagtg ttcggtcgga aggggcctcg tggcggaacg gcagacgcgt
      541 cggtcttagg agccgatgtc cgaaaggacg tgggagttcg agtctccccg aggccagtgt
      601 tcaactttat ccgatgttgc cacaacggtt ttcgtcaagg cacctgtggc acgtcgactc
      661 ggtcggacgg cggcggtgcc aagaagatcg tggctggaaa acatttgaca gcaacgtccg
      721 gtgcgttacg atagtagtcg agtaatctgc cccgcgtcgt ccctgtcgag acgctttggc
      781 cgcggttgga gccgatctgt cggccccccc ttccatccgg cgtattccta ccgtgttgcc
      841 gcttctttgt tcgtcgtcct cgggacgtac aaaacctgcc attggtcatg tcggcaccgc
      901 gccggtcttg agggcgattg cgccaccgcc ctccggtatc gaccaagcgc ggaccggccg
      961 gcctgagatc gacctcgagg aaggaagcga tcatgatcgg cttgcgggag ttggcgttgg
     1021 tcgccgtcgt ggtcctggtg ctgtacggtc gaactggtgt cgtcaagagc cgccagttcc
     1081 agacgatcct cccgtggatt tcgcccttca agcggccacg gggcaagacg ggcgcaatga
     1141 attctccgcg agcacgctcg gcgagcgcca ctgcggaagc aatgcttccg cgccccaaac
     1201 cgttcctgct gcgaggaaac cgcttattct ggttcttgac gatcctggcg gccacggccg
     1261 tggcggcctg gattgtcact cgcgtcatca tcgtgagcac cgccggaccc ggggtcactc
     1321 actgaaacga aattgtccgc cgagcgcgca cccaacgggc tcgattccgt cgaattccgc
     1381 gttaagacct gcaaatcgct ttgatggagg tttccgtcca tgatgccctt cgcgtttctg
     1441 ccgactttcg gcccgtggga gatgatcatc gtcggtatgg tatgcctctt gatcttcggt
     1501 aaccgtctgc ccagcgtgat gcgatcgcta ggcaagagcg tgaccgagtt caagaaaggc
     1561 atctcgggga tcgaggatga cctcgaccag gccgtgaccg ccgacaagaa gccaaccccg
     1621 cccaacgcct gagaaaaccg tcgcgcgaac cgccggtcat gccggctcct cgacgctttc
     1681 aacttacgga gcgcggtcgc cgcggtcgcc atccgaaaat tctaacagat catgatattt
     1741 aaactcaatt ggagagtgcc ccatgttcaa tcttggtccg atggaaatgc tcatcgttat
     1801 gggcattgcc gtcctgctct ttggcaaacg gctgccggaa gtggggcggt cgttgggtaa
     1861 agggatcgtc gaattcaaga agggactgag cggggttggt gacgaattcg atatttccgg
     1921 gacaggcacc gggcgttcgt cgacgggcta cggatcgtcc tggcgtgccg acgaccggcc
     1981 atcgggcgag tcgagctatg ccgatgcgtc cgttcccaag tttgagctgc cgggagcggc
     2041 gtcgtctgcg gagccagtga gttccccgca cacggaattt caggcggacc agccccgcta
     2101 ctgatcgtgg ccaacccaaa ttgacgcctt aggtgccttg tccttgggct agggcgcggc
     2161 gcgccaagcg attggtctgg cgcgcctggg cggcgagggc ggctcggtgg ccagcatcgt
     2221 tatcgatcaa aaggggcgga accggccgcg gacgccggcc cgactcgtcg agggctacat
     2281 agagcgcata gcccacggcc actttccggc gctctgacac ctcgatcggc tcggcgaaga
     2341 tctcgatctt ggtctcgatc gatgttctgc ccacgtaagt cacctcggcc gagaactcga
     2401 cccgctcgcc aacgtaaacc ggactgagaa agatcatcga atcgatcgcc gccgttgtaa
     2461 tctgcccttt gccggcgtgc cgtagcgcgg cgatcgcgcc gcattcgtcg gcgagcttca
     2521 agatcacgcc gccgtggagc gtgccgaacg cattggcctg accgggctca gtgatgatcg
     2581 ccagactcac ccgggaaaaa gagctcggtc gaggggggat ctcctccgtc aacatttatc
     2641 gcctcctatt cttgcacacc agtttatcgc gacgggccgg ctagcgggta ggcacttcga
     2701 cgcgggccag gcgctcactg ccttactttg atcgacgagc aatgatccgg ttcgcctgag
     2761 gccccgcccc atgctgatag accagttcaa cgagctgatc gaccctgtca ttcgcgccgg
     2821 cggttcggtg ctcgaaatcg gcgaagagtt tcgtgaaccg ccgctcgatg tgctccgcta
     2881 ttatcaccgg cccgtgtcaa tgaactgggt cccggtcttg gggaaagcgc acagcgtcgt
     2941 ggcactcgtc cagcagccgg tcgacatcga tggttcccgt gcaggccaag aacgtgtttt
     3001 gacccggttg gcgatggccg tcaacggccg ttttccgccc tggcgggggc tggtcatcgg
     3061 cttgacggct ctggttttga cccctgagcc gatcgggccg ggcgacgacg ccatcctgcg
     3121 cgacgtgctg gaggtcaagc tcaagcggat gcgggttgta ccgttcgcct tcttacgcgt
     3181 taacctcggt caagaggcga tcgccctggc gattcactcc agccccgaca gcctctttcc
     3241 cgagcccatg ctgttgggcg atgcgctttg cgaacgattt cgccggtacg ttcccttgat
     3301 cgacatgtga acgctcagat ggatcagttg accgcagacg gaggcgcaga gggagggccg
     3361 ggatagtccc aatcaaacga tacttgaaag ggcttccacc acggatccac gccggttgca
     3421 gggtcgacgt ggcggcccag tccaccagca gatggaggat cgatcgcgta ggtcagtttg
     3481 aacgagccgg ctc
//
LOCUS       DATAQL010001343         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3649747, whole genome shotgun sequence.
ACCESSION   DATAQL010001343 DATAQL010000000
VERSION     DATAQL010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3649747"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VHS97_23130"
     CDS             <1..1161
                     /locus_tag="VHS97_23130"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX3451166.1"
                     /translation="DRGTGLPHVISCSPDDLAAAKTSLASGDTRLMPALQQLLRDADQ
                     GLRQAAVSVLEKETSVDGAAPRDYVSFAPYWWPNPNSADGLPYIRKDGQHNRDRRALG
                     DVGNFSAVKRSVSALALGYYFTDDTRYARHAADLLRAWFLDPKTGMNPNLNHAQAVPG
                     VATGRPAGLIEFRDMPQLLDSLGLLERSPDWGEPDRKAMRAWLTSYYDWLTTNPMALK
                     EQKAANNHGTWYDVQAAALALYIDRPDDARRIAKEFGPNRIASQVEVDGRQPRELARV
                     DSWGYSTFNVAAMIGMAELAERVGVDIWRFQTGDGRSIKAAIGYLTPYLEEGRKWPHD
                     QGGSGSIRRGQLSSPLLRAMRGLGPDSYERFYLSFPRSEREARRERLLYDRR"
     gene            1238..2248
                     /locus_tag="VHS97_23135"
     CDS             1238..2248
                     /locus_tag="VHS97_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451167.1"
                     /translation="MSTLERRAFTLIELLVVIAIIAVLIALLLPAVQAAREAARRIQC
                     VNNLKQMGLAVMNYESTNSSLPPTGNAVVSNSFSMKPRILNYMEQTSAYNAINQSFVY
                     SDATNWTVRTIQIQSFLCPSDANVPSSTATLNGVVGQIGYGSYPNSIGTLFWNQNMLD
                     GPAYRIGGEPNVTLASVTDGTSNTAIWSEWVRGMYKVATPGLHQVYIVPYNHGIAIPL
                     PTLSAACQASILYYDAVVGGSTTVAWDQKGDDWMHNNCAQGGCYSHTNTPNTKACNFQ
                     DETSDHTYYSIVGASSNHPGGVNVGFLDGSVRFIKNSVAQPVWWGIATKAGGEVLSSD
                     SY"
     gene            2443..2580
                     /locus_tag="VHS97_23140"
     CDS             2443..2580
                     /locus_tag="VHS97_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451168.1"
                     /translation="MRVGAAAMAFPGQSAFIPKLSIVGNVTLRDVRENPVVALFGTRE
                     P"
BASE COUNT          535 a          884 c          813 g          507 t
ORIGIN      
        1 gaccggggaa cagggttgcc tcacgtcatc tcctgcagcc cggacgacct ggccgcggct
       61 aaaaccagtc tcgcttcggg cgacacacgt ctcatgccgg cattgcagca actgctccgg
      121 gacgcggacc agggactgcg ccaggctgcc gtatcggtct tggaaaaaga gacttcggtc
      181 gacggagccg ccccccgcga ctatgtcagc ttcgcgccat actggtggcc caatcccaac
      241 tccgccgacg gcctgcctta tatccgcaag gacggccaac acaatcgtga ccggcgcgca
      301 ctgggcgacg tgggaaactt ttcggccgtc aaaaggtccg tcagcgcgct tgcgctcggc
      361 tattacttca cggacgacac ccggtatgcg cggcacgccg cagatctgct gcgggcatgg
      421 ttcctggacc cgaaaacggg gatgaatccg aacctgaatc atgcgcaggc ggtgcccggg
      481 gtcgccaccg gccggcccgc gggactgatc gaatttcgtg atatgccgca gttgctcgac
      541 tcactcgggt tgctggagcg gtccccggac tggggcgagc ccgatcgcaa ggcgatgcga
      601 gcgtggctaa cgagttacta cgactggctc accaccaatc ccatggccct caaggagcag
      661 aaggcggcca ataaccacgg gacctggtac gacgtgcagg ccgcggccct ggccctctac
      721 attgaccggc cagacgacgc ccgacgcatc gccaaggagt tcggcccaaa tcgcatcgcc
      781 agccaggttg aggtcgatgg gcggcagccg cgagagctcg cccgggtcga ctcctggggt
      841 tatagcacct tcaatgttgc ggcgatgatc ggcatggcgg agctcgctga gcgcgtgggc
      901 gtcgacatct ggcggtttca aaccggcgac ggccgaagca tcaaggccgc aatcggctac
      961 cttactccct atcttgaaga gggccggaag tggcctcacg accagggcgg ttcggggagc
     1021 atccggcggg gacaactgag cagcccactt ctccgcgcga tgcgcgggct cggccccgat
     1081 tcgtatgagc ggttctattt gtcgtttccc cgatcggaac gtgaggcccg ccgcgagcgc
     1141 ctgctctacg accggcgctg accggccttc gcgaaccgac cggctagttc ttgtcgtgct
     1201 gtacattatc catttctgag tactccgagg ttacatcatg tcgactcttg agcgtcgcgc
     1261 cttcaccctg atcgagttgc ttgtcgtgat cgccatcatc gcggtattga tcgctctctt
     1321 gttacccgcg gtgcaggcgg cgcgtgaggc ggctcgccgc atccaatgcg tcaataacct
     1381 gaagcagatg ggcttggccg tcatgaatta cgagagcact aactcgtctc tgccgcccac
     1441 gggcaatgcc gtcgtctcaa actctttctc gatgaagccc cgaatcctca attacatgga
     1501 gcagacctcc gcttacaacg cgatcaacca aagcttcgtg tacagcgacg cgaccaactg
     1561 gacggtccgc accatacaga tccaatcgtt tctgtgcccc tccgacgcga acgtgccgtc
     1621 ttcaacggcc acgctcaacg gagtcgttgg ccagatcggg tacggcagct acccgaacag
     1681 cattggcacc cttttctgga accagaacat gctcgatgga ccggcctacc gcatcggtgg
     1741 tgagccgaac gtcaccctcg ccagcgtcac cgatggcacc tcgaacacgg cgatctggag
     1801 cgagtgggta aggggaatgt acaaggtcgc cacgcccggg ctgcaccagg tctatattgt
     1861 gccctacaat cacggtatcg ccatcccgct ccccactttg tccgcggcgt gccaggcttc
     1921 gatcctttac tacgacgccg ttgtcggggg ctcgacgaca gtggcgtggg accagaaggg
     1981 agacgactgg atgcacaaca actgcgccca gggaggctgt tacagccaca ccaacactcc
     2041 gaacacgaag gcctgcaact tccaggacga gacctcggat cacacctatt acagcatcgt
     2101 cggcgccagc tcaaaccacc ccggaggcgt gaacgtgggt ttcctcgacg gctcggtccg
     2161 gttcatcaag aacagcgtcg cccaaccggt ttggtggggc atcgccacca aggcgggagg
     2221 cgaagtcctg agctccgaca gctactgacc gtcccatcca ctagccgatg tgccggcgat
     2281 tcgtcggcca cgacggtgtc gtccaggcag ccgtcggtga actcgaccag ccggttgcgg
     2341 tggtccgtca gaacgagata ctcgtcctcg ctccatcgac cctgcggcgg aagtatttcc
     2401 tcgagcacat ccttccattc ttgagcagaa ggaagggcgg tcgtgagagt cggtgcagcc
     2461 gccatggcgt tccccggaca aagcgctttc attcccaagc tcagcatagt cggaaatgtc
     2521 acgctccgcg atgtgcgtga gaatccggtc gtggcgttat tcggcacgcg ggaaccgtga
     2581 tgcgaatcgc aatcgtatcc gacatccacg gcaatcgtac cgcgtttgaa gcggtcctgg
     2641 cggatctgcg gcatacctct cccgatttga tcctccacgg cggcgatctg gctcacggag
     2701 gagcaaatcc cgcggagatc gtggatcgca ttcgcgata
//
LOCUS       DATAQL010001344         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3344881, whole genome shotgun sequence.
ACCESSION   DATAQL010001344 DATAQL010000000
VERSION     DATAQL010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3344881"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..>1733)
                     /locus_tag="VHS97_23145"
     CDS             complement(376..>1733)
                     /locus_tag="VHS97_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451169.1"
                     /translation="FKALIDCRLGRFDQAQAEIRQLLAQTKDDSLPSNVYWIVGSELE
                     NHAATHDLAVTAYETGSAKLAAENSNLGLNFDYGPGKRLVSIYARDNRLEDARKLLMD
                     VPKTDNTQGYPDGYFEQMKMQSLGSAAGELLKLGFAADAVTLYNESIGMAKEIPADSP
                     NYIGNREGLLQQQRDGLTRALDELRPEDLAASVTRMIASSEAAAPKKNGDANHGSSKT
                     NKGDALLNLVVLVHPRELDKATIRSLLADTIAASGSASSPNELKARDLLAATLEQSRK
                     IHPDDLSLAIGEALLALGSGETKRIEPALARLDELVEKNPLEVLEAGGRANSRQRALA
                     AKQIPLWLVARACWKQKNATDFKVVAEKLAARAQEAARRQSENSSLLAMLREQGELAL
                     ARGDRASAEGAWSQMLKLVVEPTDRTVKKPPAKPTNPAATAAAPRAIVTGQPRAKAAG
                     P"
BASE COUNT          294 a          544 c          536 g          359 t
ORIGIN      
        1 gatcgaccca gaccgagccc gtcgccaaga cgtcgccgga cgccaagccc aaaacctgga
       61 cgttttcgat ctcgcttggc agggaaaagc cgctccagat caaggggacg atctcggtcg
      121 acgatcacgc ggccacggca ccttgaggat cgggccacgg ggaaatcccg gcttcttgag
      181 gcccatggat cgagtcggtg gctatcgaga acgcaggtgg cgggagtttt gtccgagacc
      241 ccagccaccc agtcgcatga ttgccatagt attcgttagg ttgatttcgc gcgccccctt
      301 tgcctcgatt gattgcccga aacaactttg cgtgagcacc agccgcttgg tccgctgcga
      361 cgacgcttta caagttcaag gtccagccgc cttggcccgc ggttgaccgg tgacgatcgc
      421 ccggggtgcc gccgcggtgg cggccgggtt cgtcggcttc gccgggggtt tctttacggt
      481 ccgatcggtg ggctcgacca cgagcttgag catctggctc caggcgccct ccgcgctggc
      541 ccggtcgccc cgcgccagcg caagttcccc ttgctcgcgg agcatggcca agagagaaga
      601 gttctccgac tgacgccgag ccgcctcttg cgctcgagcc gcaagttttt cggcgacaac
      661 cttgaaatcg gtcgcgtttt tctgcttcca gcaggctcgg gcgaccagcc aaagcgggat
      721 ctgtttcgca gccagggcgc gctgacgcga attggctcgc cctccagcct caagaacttc
      781 tagcgggttc ttctccacga gttcgtccag tcgcgccagc gcaggctcga tccgcttggt
      841 ctcgcccgat cccagcgcca gcagagcctc gccgatcgcc agcgaaaggt cgtcgggatg
      901 gatcttccgc gactgctcta gggtggccgc tagcaggtcc cgcgccttca actcgttggg
      961 cgacgacgca ctccccgagg ccgcaatcgt gtcggccagc aggcttcgaa tcgttgcttt
     1021 gtccagctcg cgaggatgga ccagcaccac caggttcaag agcgcgtcgc ccttattcgt
     1081 cttggaggag ccatgatttg catcgccgtt tttcttgggg gctgccgctt cggaactggc
     1141 gatcattcgg gtgacactcg ccgccagatc ttcgggccgg agttcgtcga gagctcgcgt
     1201 caacccatcg cgttgctgct ggagcagccc ttcccgattg ccgatgtagt tgggactatc
     1261 ggcggggatc tccttggcca tgccaatcga ctcattgtag agcgtgaccg cgtcggcggc
     1321 gaaaccgagc ttgagcaact caccggcggc cgatcccagg ctttgcatct tcatttgctc
     1381 aaaatatcca tcaggatagc cctgggtgtt gtcggtcttg ggaacgtcca tcagaagctt
     1441 tctggcgtct tccaggcggt tgtcgcgggc ataaatcgag accaagcgtt tgccgggccc
     1501 ataatcgaag ttgagcccca aattcgagtt ctcggcggcg agcttggcac taccggtttc
     1561 gtaggcggtc acggccagat cgtgggttgc ggcgtggttt tcgagctcgg aaccgacgat
     1621 ccagtagacg ttggacggaa gcgagtcgtc cttggtctgg gcgagcaact gccggatctc
     1681 cgcctgggcc tgatcgaaac gcccgagccg gcagtcgatc agcgctttga aga
//
LOCUS       DATAQL010001345         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2122525, whole genome shotgun sequence.
ACCESSION   DATAQL010001345 DATAQL010000000
VERSION     DATAQL010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2122525"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VHS97_23150"
     CDS             <1..636
                     /locus_tag="VHS97_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451170.1"
                     /translation="AWAAYRGDWVPVLNFLREHGFHDKQTMINYVAEFSQLPALDLVP
                     SNRRVVPLKRQDLPRLVELGSDLFGEVDVPAVERFYWNNPFYNFPDTIFAWKDSENDE
                     ILGAFLLVVSDRFADPTKIDAAMPCFRLGAFGTERERHKRVTGLFSCAFANEAEGDQM
                     FPAALAATRGLSRLTHIAAQAPSTATSLCAWYDRYFQRQASFPILSRRLSG"
     gene            770..1045
                     /locus_tag="VHS97_23155"
     CDS             770..1045
                     /locus_tag="VHS97_23155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451171.1"
                     /translation="MNTWTVILSTILVICGPLVLYGFHRLGLWMEEQGYIYYLHKKPK
                     GSAAGSFVALQKMIEPRAEYVIQVTRADHKLGEEKPSGQGDPDRSRQ"
     gene            complement(1075..1977)
                     /locus_tag="VHS97_23160"
     CDS             complement(1075..1977)
                     /locus_tag="VHS97_23160"
                     /inference="COORDINATES: protein motif:HMM:NF042562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6687 family protein"
                     /protein_id="HEX3451172.1"
                     /translation="MTALERRFQYIEESYEGEIRAISCDGLVPGAALDLTHWQGNRTP
                     PQYKADTSTEIALNFVGSPESGEQWANAVVVNNHFDTDGILSAWVLLDPEKAMAHGAL
                     LVAAAESGDFDEWPALDRGLWLDAAIRQLAAGAADDEAAYQLVLPQLPELIHDLDQRR
                     DLWGREWDDLQSAVAALESGGLRAERLHMLGVMIHAIGQPEVPGALLARRFLPAARRY
                     LLAFGREGGRYDYRYERPHYAWADTVIRPVLPQPDAGALAKALGLEWTDEGLPGMTGI
                     VRTSRPVEEQPDALARRLLEIDLT"
     gene            complement(1996..2307)
                     /locus_tag="VHS97_23165"
     CDS             complement(1996..2307)
                     /locus_tag="VHS97_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451173.1"
                     /translation="MSEMTSYTYLARREGSRYEQYFFKGRNLRAETLYRATIGSEPMT
                     PAEVADDYGVPVAAVLEAIQYSVQNAVLLQREREEDWDESQARGLVVSRPLTSGSRGA
                     G"
     gene            2442..2996
                     /locus_tag="VHS97_23170"
     CDS             2442..2996
                     /locus_tag="VHS97_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX3451174.1"
                     /translation="MQSLKGHLLIATPQLESTIFARAVILMLEATDEAAAGVILNQPI
                     KTTLTELSGKIFAEDFEWDKPISLGGPLASSLMVLHSEEHLADKEVIPGVYFTAEPTK
                     VHELVERRLEPSLVLANYSAWGPGQLDGEIRRDSWLTLPATSKQVFWNGEKELWKAVW
                     SEVRTSKLAHMLRIRELPVDPRLN"
     gene            complement(2956..>3217)
                     /locus_tag="VHS97_23175"
     CDS             complement(2956..>3217)
                     /locus_tag="VHS97_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451175.1"
                     /translation="WIETKEHQAVDAFEPLIARPDVALVLLAIRWSSHAFGDVKQICD
                     RHGKLLVRLPGGYNPNQVAAQILAQSSAQLSSDVGLQAARES"
BASE COUNT          646 a         1012 c          900 g          659 t
ORIGIN      
        1 gcctgggccg cttaccgagg cgactgggtt cctgtcctca acttcctccg cgagcacgga
       61 tttcacgaca agcagacgat gatcaactat gtggccgagt tctcacaact ccccgcgctc
      121 gatcttgtgc catcgaaccg gcgggtcgta cctctgaaac gccaggatct acctcgattg
      181 gtcgagctcg gatccgatct cttcggcgaa gtcgatgtgc cagcggtcga gcgattttac
      241 tggaacaatc ctttctacaa cttccccgac acgatctttg cctggaaaga cagcgagaac
      301 gacgagattc tgggagcatt cctgctcgtc gtcagcgatc gtttcgccga cccgaccaag
      361 atcgacgcgg cgatgccctg ctttcgtctt ggcgccttcg gtaccgagcg cgagcgacac
      421 aagcgtgtga ccggcctttt ttcgtgcgcc ttcgcgaatg aggccgaggg cgaccagatg
      481 ttcccggctg cgctggcagc gacccgaggc ctatccaggc tgactcatat cgcggcccaa
      541 gctccctcga ctgcgacttc cctctgtgcc tggtatgacc gctacttcca gcgccaggct
      601 tcgttcccca tcctgtctcg gcggttgagc gggtagttcg ccgccctcgt gaatcggcgg
      661 atcgtcgatt caacgactga ctcatttccg caagttcgaa caattctcga cgcccaagct
      721 gtccgttaca tgacggaatc tcacgatttc atcgcccgag gatcctgcca tgaacacgtg
      781 gaccgtgata ctctcgacca tcctcgtcat ttgcgggccg ctggtgctct atgggttcca
      841 tcgtctcggc ctgtggatgg aagaacaggg ctacatctac tacttgcaca aaaagccaaa
      901 aggaagcgcg gccggctcgt ttgtggccct acaaaagatg atcgagccgc gggcggagta
      961 cgtcattcag gtcacgcgcg ccgaccataa actcggcgag gagaaaccgt ccggacaagg
     1021 tgatcctgat cggagtaggc agtagtgtct tcgctgccgc ctgctcgtgg cctgtcacgt
     1081 caaatcgatc tccaagagtc tgcgggccag agcatcgggt tgttcttcca ccgggcgaga
     1141 ggtacggaca atgccagtca tgcctggtaa accttcgtcg gtccattcca atcccagtgc
     1201 tttggccagc gcgccggcgt cgggttgagg caagactggg cgaatcacgg tatcggccca
     1261 ggcataatgc ggacgttcat agcggtaatc ataccggccg ccttcgcggc cgaaggccag
     1321 cagatagcgc cttgcggcag gaaggaaccg tcgagcgagg agcgctcctg ggacctccgg
     1381 ttgtcctatc gcgtggatca tcacaccgag catgtgcagc cgttctgcgc gcagaccgcc
     1441 tgactcgagc gcggcgaccg ccgactggag atcgtcccat tcccggcccc aaagatcgcg
     1501 acgttgatcc aggtcatgga tcagctcggg caactggggg agaaccagtt gataggcggc
     1561 ttcgtcatcc gccgccccgg cagcgagctg gcgaatcgcg gcatccagcc aaaggcctcg
     1621 atcgagcgcc ggccattcgt cgaagtctcc cgactcggcc gcggcgacca acagtgcccc
     1681 atgggccatc gctttttccg gatcaagcag aacccacgcc gatagaatgc cgtcggtgtc
     1741 aaaatgattg ttgaccacta cggcgttcgc ccactgctcg ccggactccg gcgaaccgac
     1801 aaaattcagg gcgatctcgg tcgacgtatc ggccttatac tgcggcggcg tgcggtttcc
     1861 ctgccagtga gtcaggtcga gcgcggcccc cggcactaag ccgtcgcaac tgatggcccg
     1921 tatttcgcct tcataagact cttcgatata ctgaaagcga cgttccagtg cagtcacggt
     1981 gaggctcctt cacgttcatc ccgcgcctcg ggaacccgac gtcagaggcc gtgatacaac
     2041 cagacctcgg gcctggcttt cgtcccagtc ctcttcccgt tcgcgctgaa gaagcacggc
     2101 attctgcact gaatactgaa tagcctcgag cacggccgcg accggtacgc cgtagtcgtc
     2161 ggctacctcg gccggcgtca ttggctccga cccgatcgta gcgcgataaa gcgtttcagc
     2221 tcgcaagttc cggcccttga agaagtattg ttcatagcgc gagccctcac gccgggccag
     2281 atacgtgtag cttgtcattt cactcatgat aggcctccaa caaccatctt acaccaattg
     2341 ggtttttggc atcacacgtg ctgcgatata attgggtggc tttcagtcga gcttcatcga
     2401 cccgcccatc gacccgtctc cgccagatag gctgttcagc aatgcaatcg ctcaaagggc
     2461 atcttctgat cgcgactccc caacttgaat ccacgatctt cgctcgcgcg gtcattttga
     2521 tgctcgaagc cacggatgaa gccgcggctg gcgtcatctt gaatcaaccg atcaagacca
     2581 ccctgacgga actctccggg aaaatcttcg ccgaggattt tgagtgggac aaacccatct
     2641 cgctgggcgg accactggca agctcgctta tggtgctcca tagtgaagaa cacctggccg
     2701 acaaggaagt catccccggt gtctacttca cagccgaacc caccaaagtc cacgagctcg
     2761 ttgagcggcg gcttgaacca tcgctcgtcc tggccaatta ttcggcctgg gggccgggtc
     2821 agctcgacgg cgagatcagg cgcgattcat ggctcaccct cccagccaca tctaagcagg
     2881 tcttctggaa cggggaaaaa gagctctgga aagccgtctg gagcgaagtc cgtaccagca
     2941 agctggcgca catgctcagg attcgcgagc tgcctgtaga cccacgtctg aactgagctg
     3001 ggcactcgat tgcgcgagga tctgggcggc aacctggttg gggttatatc cccctggcag
     3061 acggaccagg agcttgccat gtcggtcgca tatctgcttt acatctccaa atgcatggct
     3121 cgaccagcgg atggccagta aaaccaaggc gacatccggc cgggcgatca acggctcaaa
     3181 ggcatcgacc gcctgatgct ccttggtctc gatccat
//
LOCUS       DATAQL010001346         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3190839, whole genome shotgun sequence.
ACCESSION   DATAQL010001346 DATAQL010000000
VERSION     DATAQL010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190839"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..723
                     /gene="rnpB"
                     /locus_tag="VHS97_23180"
     ncRNA           190..723
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHS97_23180"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(807..1202)
                     /gene="rbsD"
                     /locus_tag="VHS97_23185"
     CDS             complement(807..1202)
                     /gene="rbsD"
                     /locus_tag="VHS97_23185"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEX3451176.1"
                     /translation="MKKGGILNPAICSLLAELGQSDELLIVDAAYPLPTDGHVIDLTL
                     TPGIPRLLDVLRAVTEELVIDAVTVPAEIKEFSPRLYQEIVKMVGETDVDEVPFHEFK
                     EQALDVKGIIRSAEFSPFASVRIVAGSAF"
     gene            1499..>2323
                     /locus_tag="VHS97_23190"
     CDS             1499..>2323
                     /locus_tag="VHS97_23190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451177.1"
                     /translation="MGMRGGGPGWAFSIPVLLLALWMVGVSVVVWRGLGPRRSRILNR
                     FVRITGGRTQNAEADVLPAGPSRLADPRLSSLRKAAESWRGATGPKRLVVDQVCLVPD
                     EVAFLEAIAAWDERSYFPILIDEPAWTLPFLRAFRPARVVRYAGRARTTKAWIDGGSL
                     LSRDGRERWLRANEAVSRAWSGPSDSGRVLPAADLPPRWLKDRPPGLVLAEPQAPMLA
                     GAVALAAGRFQPLVRLQLNTGGPLRIGESAQTRRFGDVLSVEQAWQLARAVEARVAS"
BASE COUNT          436 a          655 c          777 g          455 t
ORIGIN      
        1 caccggcgaa gatgctaaag ttgaaccatg ccgtataact cgtttctttg tcctgtcggg
       61 gcggacgatt gagacgcatc cgcgcgtctg ccagaatccg ttcgcgcacc ctggccaacg
      121 tcggtgatta acgcgagtgg aacgcgggtt gttgtgggcg actcttgtgc gtatgatcag
      181 atagagttcg tggcgggcgc gatgatcgcc gtggcgcttg gacttggggt gttgagccgg
      241 gaaaccggtc caccgctcga ggaccgaggt cgtgccacgg aggaaagtcc ggactcctaa
      301 gggcgcgatg gtgggtaacg cccaccgtct gagagcgcag tgcgtggctc ggatagggaa
      361 agtgccacag aaaacagacc gccccggacg tggcgcttgc cgtcacgatc ggggtaaggg
      421 tgaaacggcg aggtaagagc tcaccagcag gccgggtgac cggcttggct cggcaaaccc
      481 catcgggagc aagaccaaat aaggaagcag aacggttggc gggacgaggc agcgcggttt
      541 atccgcgtga atggccctgt caatcattca cggatcggcc cgatccgtta cgacttccgg
      601 gtaggtcgct cgaggcgtca ggtaactgac gtcccagaaa aatgatcatc cggtctcggt
      661 cgcgacgtga aaacgtcgct gcagagattc gacaaaatcc ggcttatgat gcccgccacg
      721 accttccttt ccttcggaga agatcgcaag agtttgatct ggtcgttctc cgcaccgagc
      781 tcgctcgagt aatcctcgct tgcccctcag aacgcgctac cggccacgat gcgaacgctc
      841 gcaaacgggc taaattcggc cgaccggatg attcccttga cgtcgagcgc ctgttccttg
      901 aattcgtgga acggaacttc gtcgacgtcg gtctcgccga ccatcttgac gatctcttga
      961 tagaggcggg gactaaattc cttgatctcg gccggcaccg tgacggcatc gatgacgagc
     1021 tcttcggtga cggcccgcaa gacatcgagc aaacgcggga tgcccggggt cagggtcagg
     1081 tcgatgacgt ggccgtcggt cggcagcggg taggcggcat ccacgattaa cagttcgtcg
     1141 gattgcccga gctcggccaa aagagaacag atcgccggat tgaggatgcc accctttttc
     1201 atgggacttg aaccttaacg agccaggcaa gacctcgttc acgccgtcag tctaacctta
     1261 ggggacgatg cgttttcgcg ggtcgggaga ttcggtgacg cgtcctggcg aggatgcctg
     1321 gaaatcgggg gttgcgggtt ccacgacctt ttggagtata acgattatga tcaacctgct
     1381 ctacccagga tgggatctag cccgaaaccc gccgtgaacg cgaagagcag gcccatcgat
     1441 gagacaacct cttgactgag aagacatgcc gccaacgaat ctgcctcgag gagcagtaat
     1501 ggggatgcgc ggcggggggc caggctgggc cttttcgatt ccggtgttgt tgctggctct
     1561 gtggatggtt ggagtgagcg tggtcgtctg gcgcggactt ggcccccgcc ggagcagaat
     1621 cctcaatcgc tttgtacgga ttaccggggg ccggacccaa aacgccgagg ccgacgtact
     1681 gcccgccggg ccgtcacgac tggctgaccc tcggctgagc tcactgcgta aggccgcgga
     1741 gtcgtggcga ggggcgacag ggccgaaacg gctggtcgtc gaccaggtct gcctggtgcc
     1801 cgatgaagtc gcttttctcg aggcgatcgc cgcgtgggac gaacggtctt acttcccgat
     1861 cctgattgac gagcctgctt ggacacttcc gtttctccgc gcatttcgtc cggctcgcgt
     1921 ggtgcgttat gcggggcgcg ccaggactac gaaggcgtgg attgatggcg gctcgctact
     1981 gtcacgggat ggtcgcgagc ggtggttgcg ggccaatgag gccgtatctc gagcttggtc
     2041 gggtccatcc gactctgggc gcgtgctgcc tgcggccgat cttcccccac ggtggttaaa
     2101 agatagaccg ccgggcctgg tcctggccga gccccaagct ccgatgcttg ccggggccgt
     2161 cgcacttgcg gccggccggt ttcagccgct ggttcggcta cagctaaaca ccgggggacc
     2221 actgagaata ggtgaatcgg cgcagactcg gcgatttggc gatgtcctga gcgtagagca
     2281 ggcgtggcag ttggcgaggg cggtggaggc gcgggtcgcc tcg
//
LOCUS       DATAQL010001347         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1969565, whole genome shotgun sequence.
ACCESSION   DATAQL010001347 DATAQL010000000
VERSION     DATAQL010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969565"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VHS97_23195"
     CDS             <1..677
                     /locus_tag="VHS97_23195"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX3451178.1"
                     /translation="ALPEVLARVKSLFDLNARPDVIASHLADDARLGCVAATYPGLRV
                     PGAFDGFELAVRAILGQRVSVTAATSLAGRLVGRLGEPIETPVPALHRLFPSPARLAS
                     VEELELRSLGISGARAVTISAIARAVLGRQIDLEPSPDPEAVIAKLQELPGIGDWTAQ
                     YIAMRALRWPDAFPAGDLGLLKAMGEKSVQRLRDAGEAWRPWRAYAAMYLWESQRMSN
                     RKDHHD"
     gene            670..1233
                     /locus_tag="VHS97_23200"
     CDS             670..1233
                     /locus_tag="VHS97_23200"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX3451179.1"
                     /translation="MIESDSLSICHSLYSSPIGDLLLTSDGEALTGLHMCQRRGKPAP
                     EPEPSCRRDDAAFQAVREQLDAYFGGELRDFEIPLRMPGTPFQRMVWAGLRAIPYGST
                     VSYAELARRIGRPGASRAVGSANGRNPIAIVVPCHRVIAADGTLGGYGGGLDRKEWLL
                     ELEGATRQSAKETHPAERLVATCLAVD"
     gene            1347..2528
                     /locus_tag="VHS97_23205"
     CDS             1347..2528
                     /locus_tag="VHS97_23205"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3451180.1"
                     /translation="MIRRRDVPLAAAAQGLAIWSGSAFAQTKRADKKETATALAPVHA
                     DERVSRILAPIRDEHHLPGLIGAVLKGDRLAAIGALGIRKIGSSEPMRVTDQVHLGSC
                     TKAMTATMIGTLIDAGKLSADTTIRQVFPKDAPEFHPEFQSVTLWHLLTHRAGLPTEG
                     PWWDLSGETTTEKRLDLLARMLGDAPQSRPGATYAYSNVGYVLAALMAEQVTGRSWED
                     LMRKRVFEPLGMISAGFGSPGHAGRVDQPWGHHPSGREIRPTREDNAPAMGPAATVHC
                     TVPDWAKFAAVHLRGSQGKPSLLKPATFRGLHTPPRGHDYAGGWLVGERSWAGGRTLS
                     HNGSNTNWYAAIWIAPARDFAVLTATNQGGAAGDKATDQSASKLIESVAFLTRQESRK
                     L"
BASE COUNT          472 a          822 c          881 g          475 t
ORIGIN      
        1 ccgcgctgcc cgaggttctg gcccgggtga agagcctttt cgatttgaac gcgcggccgg
       61 acgtgatcgc ctcgcatctt gcagacgatg cccgattggg gtgtgtggcc gcgacttatc
      121 ctggattgcg agtaccggga gcgttcgacg gtttcgagtt ggcggtgcgc gccatcctcg
      181 gccaacgtgt gtcggtaacg gcggctacga gtctggccgg ccgcctggtt ggccggctgg
      241 gcgagccgat cgagacgccg gtcccggcct tgcaccggct gttcccttcg ccagcacgac
      301 tggcgtctgt cgaggaattg gagttgcggt cgctcgggat cagcggagct cgcgccgtaa
      361 ccatcagcgc catcgctcga gccgtgctcg ggcgccagat cgacctcgag ccgagccccg
      421 acccggaggc cgtgatcgcc aagctccagg aactgccggg aatcggcgac tggacggccc
      481 agtatatcgc catgcgcgcc ttgcgctggc cggacgcatt ccccgctgga gatttgggat
      541 tgctgaaagc gatgggggaa aagtcggtgc aacgtctccg ggacgccggc gaagcctggc
      601 gcccttggcg tgcctacgcg gctatgtatc tgtgggaaag tcaacgtatg agcaatagaa
      661 aggaccacca tgattgaatc tgactccctg agtatatgcc actctttgta ctccagtccg
      721 atcggcgacc tgctgctgac gtccgatggc gaggcattaa caggactgca tatgtgccag
      781 cgcagaggga agcccgcgcc ggagccagaa cccagttgcc gacgggatga tgcggcattt
      841 caggcggtcc gcgagcagtt agacgcctac ttcgggggcg agctgcgcga tttcgagatt
      901 ccgcttcgga tgcctggaac gccctttcag cgcatggtgt gggcgggatt gcgagcgatt
      961 ccttacggat cgaccgtgag ctacgccgag ctcgcccgga gaattgggcg accgggggcg
     1021 tcgcgagccg tcggatcggc gaatggtcgc aacccgattg ccatcgtggt tccctgtcat
     1081 cgcgtcattg ccgccgatgg aaccctgggg gggtacggcg gcggcctcga ccgcaaggaa
     1141 tggttgcttg agcttgaagg ggccacccgc cagtcggcga aagagacaca cccggcagag
     1201 cgcctcgttg cgacgtgtct cgcggtcgac taagctctta tttagaggtg ctgacgccgg
     1261 cgcactataa agaactctgc ggccgaatgc tcggccggtt cgagatagag gcagcgtcga
     1321 ccccggaata agcgagcgaa gagatcatga tcaggcgtcg cgacgttccg ctggccgcgg
     1381 cggcgcaagg gctggcgatc tggtccggct cggcgtttgc ccagacaaaa cgtgccgata
     1441 agaaggaaac tgcgactgcc ctggcgccgg tgcatgccga tgagcgggtc agccgcatcc
     1501 ttgcaccgat tcgcgatgag caccacctcc ctggattaat cggtgcggtc ttgaagggcg
     1561 atcggctcgc ggcgattggg gccctgggaa tccgtaagat cggctcgtcc gagccgatgc
     1621 gggtcacgga ccaggttcat ctcgggtcgt gcaccaaggc catgacggcc accatgatcg
     1681 gcactctgat tgacgcgggc aagctttcgg ccgacacgac gatccgccag gttttcccca
     1741 aggacgctcc ggaatttcat cctgaatttc aatccgtcac gctctggcat ctcttgacgc
     1801 acagagctgg tcttcccacc gagggcccct ggtgggacct atcaggcgag accacgaccg
     1861 aaaagcgcct ggacctgctc gccagaatgc tcggcgacgc gccccagagt cggccgggag
     1921 ccacgtacgc ctactcaaac gttggctacg tcctggccgc attgatggcc gaacaagtga
     1981 ccggccgttc gtgggaggat ctgatgcgca agcgggtgtt cgagccgctg ggaatgatat
     2041 cagcggggtt tggatcgccg ggccacgccg gtcgagtcga ccagccctgg ggccatcacc
     2101 cctcgggaag agagatcagg ccgacccgcg aagacaacgc gccggcgatg gggcccgccg
     2161 cgactgtgca ttgcacagtg cccgactggg ccaagtttgc cgcggtgcac ctgcggggat
     2221 ctcagggaaa gccctcgctg ttgaagccgg ccactttcag gggcctgcat actcctccgc
     2281 ggggccacga ctacgccggc ggctggctcg tcggcgaacg gtcatgggcc ggcggtcgca
     2341 ccctgtcaca taacggtagc aacacaaact ggtacgccgc gatctggatt gcgccggcgc
     2401 gcgatttcgc cgtcctcacc gcgaccaacc agggaggcgc cgctggcgac aaggccactg
     2461 accagtcggc tagcaagctg atcgagtctg tcgcatttct gacccggcaa gaatcacgaa
     2521 aactctgagg cccggcgcct gtgtggctcg cgattcactt ggatccagac ccggagttgc
     2581 cgctttcgag gtcgatccgg ccccagacgt gatcgccgct cacggggttg gatcgggcga
     2641 cctgtccgtc
//
LOCUS       DATAQL010001348         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2274487, whole genome shotgun sequence.
ACCESSION   DATAQL010001348 DATAQL010000000
VERSION     DATAQL010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274487"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(679..2055)
                     /locus_tag="VHS97_23210"
     CDS             complement(679..2055)
                     /locus_tag="VHS97_23210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3451181.1"
                     /translation="MGNLSRRSVLRTSLAATAVGTLARPYITNAAATTAEVWWAQGFV
                     PEEDTAIKKVVADYEKASGNTIDLTITPFAPQRQKIVAAVQSGIVPDVFPNNPAEITA
                     LYAWDDKLIDVSDVVETQKEEYTETALLNTFCYNRVTKDRSFYGVPDTTAALPNHVWR
                     PLVEKAGFKIEDIPKTWDAYYSFFKDVQKSLRKQGLRNVYGLGLNVTTNGVDPNNVFN
                     YFVIAYGGQNLVTKDGRLHLDDPQVKEAVIKAMTYPATAYTEGFVPPGAINWNDADDN
                     NAFHAKQIVMDLDGTISTEVAVLSQGKKEDYDDIVTMGLALSNDGKPVPSHATNNTLL
                     VPKGAKNVAVAKDFLKYIIQPKVNNERLKTGLGRNIPCMPSIVKTDHWWFDDPHRAAY
                     TTQGLLSPTVPQFWAFNPAYAQIQNEHVWMQGWMDIIQSGMTPQAAAEKAFKRVEEIF
                     AKYPIAQA"
     gene            2400..2696
                     /locus_tag="VHS97_23215"
     CDS             2400..2696
                     /locus_tag="VHS97_23215"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX3451182.1"
                     /translation="MPHSGPSALTVGTALLAPEADHPDGESTVAGMRIGSRRDDGCLQ
                     FDLMLPKEDDKHVFLVEKWCDQEALDAHAKGPMPGNTYKDWIVGRKMERGAPAD"
     gene            2710..>2910
                     /locus_tag="VHS97_23220"
     CDS             2710..>2910
                     /locus_tag="VHS97_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451183.1"
                     /translation="MGFTLRIFRMWLGAVLIACGAATGAVADGTTMLAGKSAKTSAET
                     APPAEIQVLLNLLADPKVQDWLK"
BASE COUNT          547 a          862 c          879 g          622 t
ORIGIN      
        1 tcgatgtgcc gtgtcttgcg gcagcccaag aacgtccagg atctcattga gccatctttc
       61 ctgatctgac attttcgtgc gcctttaccg agctttcgga cttcaagtta ggtaagcgcg
      121 ccgaccgtca tgaaatgcaa actgtgccac agaagcgcag cggatgagat cgatatagcc
      181 tgctgcgcga tgatcgccta ctcatccgag gaccccgctc acccggttga aaatatgctc
      241 gatggaccgt ccggcccggg agggacgcgc tgggtcagcg cgcgtcccga cacggctgaa
      301 cacatcgtag tggaatttgg accagccgcg ggcgatctcg cggctcgtct acgaagtcga
      361 ggagacgatg cgcgaacgca cccaggtgaa ggcatttcat cggtccatac ccagcgcttc
      421 agaacaggag cggattggag catgaacaag gttgactgat gaagtggtcg gttgaatccg
      481 gggggccgcc gtcggactcg tccggttgat tttatccact agcgacccta ttcgactgaa
      541 ctggggtcgt cggggcattc gaactcgcct gacaacccca gtaatgccgc gcggagctca
      601 attccgcgcc cacgacccct aggtaaccgt tagccgcgtc ggcgcggcga ataatgacgc
      661 ccagcagctc gattcgcttc aggcttgggc gatggggtac ttcgcaaaga tctcctcgac
      721 ccgcttgaag gccttttctg ccgcggcctg gggtgtcatg ccgctttgga tgatgtccat
      781 ccagccctgc atccaaacat gctcgttctg gatttgggca taggccgggt tgaacgccca
      841 gaattgcggc accgtcgggc tcagcagccc ttgggtggtg tatgccgctc ggtgcggatc
      901 gtcgaaccac cagtggtcgg tcttcacaat cgagggcatg cacggaatat tgcgtccgag
      961 ccccgtcttt agccgctcgt tgttgacctt gggctggata atgtacttca agaagtcctt
     1021 ggcgaccgcg acgtttttgg ctcccttcgg gaccagcaag gtgttgttag tggcatggct
     1081 cggcaccggc ttgccgtcat tgcttagcgc caggcccatc gtgacgatgt cgtcgtagtc
     1141 ttccttcttt ccttgtgaca gcaccgcgac ttcggtcgag atcgtgccgt ccagatccat
     1201 cacgatttgc ttggcatgga aggcgttgtt gtcgtcggca tcgttccagt tgatggctcc
     1261 cggcggcacg aagccttccg tgtaggccgt ggccggataa gtcatcgcct tgatgaccgc
     1321 ctccttgacc tgtgggtcat cgagatgcaa cctgccatcc ttagtaacga ggttctggcc
     1381 gccataggcg atcacgaaat agttgaagac gttattgggg tcgaccccgt tggtcgttac
     1441 attcaacccg aggccgtaga cgttgcgcag gccttgttta cgcaggctct tctgcacatc
     1501 cttgaagaaa ctgtaatagg catcccaggt cttcgggatg tcctcgattt tgaagcctgc
     1561 cttctcgacc agcggtcgcc agacatggtt cggcagcgct gcggtcgtgt caggcacgcc
     1621 ataaaagctg cggtccttgg tgaccctgtt gtagcaaaaa gtgttgagca gcgcggtctc
     1681 ggtatattcc tccttttgcg tctcgacgac gtcgctgacg tcgatcagct tgtcgtccca
     1741 ggcatagagc gcggtgatct cggccgggtt gttggggaac acatctggga cgatgccgct
     1801 ctgcaccgcc gcaacgatct tttggcgctg cggggcaaac ggcgtgatcg tcaggtcaat
     1861 cgtgttaccg cttgccttct cgtaatcggc gacgaccttt ttgatggcag tgtcctcttc
     1921 ggggacgaag ccctgcgccc accacacttc cgcggtcgtg gccgcagcat tggttatgta
     1981 aggccgcgcc aacgtcccga ccgctgtcgc agccaacgaa gtgcgaagca ccgagcgcct
     2041 gctcaggttt cccatgtccg ccttccttcc tgttcatgcc gcgcgatccg ccccgcgcgt
     2101 gcaatgtgtt tatgaacgca ggttgagaca gcccgctccg acagtcaacg gattctttgg
     2161 caggaagaca tcggcagagc gagcaacctg ccgccacctg agttgagtgg agacccgcgc
     2221 gttatttggc tggacgatgt cgctcttgag ggcgctttgg ggtccaaacg caaccgctca
     2281 acaggaaccg agagaatgac cgcaaacggc gcatccttcc cgttcccgct cgctctggcg
     2341 gaggtcggct aactgaacgg acaccggccg ttcagcttag gcggcgggaa cgggtcaaag
     2401 tgccccacag cggaccctca gccctcacgg taggaaccgc tcttcttgcc cccgaagctg
     2461 accatccgga tggcgaaagc accgtagcgg gcatgcgcat cggctctcgc cgtgatgacg
     2521 gctgcctgca attcgacttg atgctgccga aagaagacga caagcacgtc tttttggtcg
     2581 agaagtggtg cgaccaggaa gcgctcgatg cccacgcgaa ggggccgatg ccgggcaaca
     2641 cctacaaaga ctggatcgtc ggccgcaaaa tggagcgtgg cgcaccggct gattagcggg
     2701 agagacgcca tgggcttcac gctgcgcatt tttcggatgt ggctcggggc ggtactgata
     2761 gcttgcggcg cagccacggg tgccgtcgcc gacggcacga cgatgcttgc cggcaaatcg
     2821 gcaaagacct cggccgaaac ggcgccgccg gcggaaatcc aggtgctgct gaacctcttg
     2881 gctgacccca aggtgcagga ttggctgaag
//
LOCUS       DATAQL010001349         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3955530, whole genome shotgun sequence.
ACCESSION   DATAQL010001349 DATAQL010000000
VERSION     DATAQL010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3955530"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VHS97_23225"
     CDS             complement(<1..1210)
                     /locus_tag="VHS97_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX3451184.1"
                     /translation="MPDVHPFRAVRYDVARVGNLSDVVAPPYDVIDTALQDRLYEASP
                     HNVIRLELNRDEPGESGSSDRYARAARMLKDWLREGVLRQDDHPAIYVYDQTFHVDGK
                     KHTRKGFMARVRLEPIGQGKIYPHEQTLAGPKADRLALYHATGFNLSPVFGLYPDAAA
                     EVQRTIEAGLRDRTPLVATDHLGVENRLWILTDQEAHTAVSGLMAAKPVFIADGHHRY
                     ETALKYRDEVAQAGQSSGHDDPSNFCLMMLVGMSDPGLLILPTHRLVSGYPGLTAAEL
                     SKRLEPQFEVRNVGSGETGCRAAWNLIDGGGDQDLLGFGTVADGEWVVARLRSDTAMD
                     RLAPEHSSDWRSLGVSVLHVLVLNELLADKGTASCRYVHSIAEVLADTAARNCDLACL
                     VPPAQMGHVES"
     gene            complement(1535..1663)
                     /locus_tag="VHS97_23230"
     CDS             complement(1535..1663)
                     /locus_tag="VHS97_23230"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX3451185.1"
                     /translation="MYQVPKEVDEWVAALKLKTMGISIDTLTSEQKKYLESWEMGT"
BASE COUNT          314 a          495 c          538 g          335 t
ORIGIN      
        1 tgctttcaac gtggcccatc tgtgcgggcg ggacgagaca agcgaggtcg cagttgcgtg
       61 cggccgtgtc agcgagaacc tcggcgatcg aatggacata gcggcaggac gcagtcccct
      121 tatcggcgag aagttcgttg agcacgagga cgtgcaaaac gctcacgcct agactgcgcc
      181 aatcagagct atgctccggg gccagccgat ccatcgcggt gtccgatcgc agcctggcga
      241 cgacccattc gccgtcggcg accgttccaa agccgagcag atcctgatcg ccccccccgt
      301 caatcaagtt ccatgcggct cggcagccgg tctcgccgct gcctacgttc cgcacctcga
      361 attgaggctc aagacgcttt gaaagctccg cggccgtgag cccgggataa cccgaaacaa
      421 ggcggtgcgt gggcaagatg agcaatccgg gatcgctcat tcccacgagc atcatcaagc
      481 agaagttaga cggatcatcg tgaccggacg actggccggc ttgggcgacc tcatcgcgat
      541 acttgagcgc ggtctcgtag cggtggtggc cgtcggcgat gaagacgggt tttgcggcca
      601 tcagcccgct gacggcggtg tgtgcttctt gatcggtcaa gatccagagc cggttttcaa
      661 cccccaagtg atcggtggcc accagcggtg tgcgatcgcg cagcccggcc tcgatcgtgc
      721 gctggacttc cgcggcggcg tcgggataga ggccaaagac gggactgaga ttgaaaccgg
      781 ttgcgtggta aagcgccagt cggtcggcct tgggaccggc aagcgtctgt tcgtgggggt
      841 agattttacc ctggccgatc ggttcgagtc gtacgcgcgc catgaacccc ttgcgggtgt
      901 gttttttccc gtcgacgtgg aaggtctggt cgtacacata aatcgcgggg tgatcgtcct
      961 ggcggagaac tccctcgcgc aaccagtcct tgagcatccg tgccgctctt gcgtagcggt
     1021 cggacgagcc gctctcgccc ggctcatcgc gattaagctc caggcggatg acgttatgcg
     1081 ggctggcttc gtatagccgg tcttgcaagg cggtgtcgat cacgtcgtaa ggaggtgcca
     1141 ccacgtcgga caaattgccg acgcgtgcga cgtcgtatcg gacagcgcga aaaggatgca
     1201 cgtcaggcat gggtcatctc gccgcggagg gtggaaatga ttagtctctc ggccaatgtc
     1261 gggatttgca atggtcgaat acgacctgaa taaaagtggc tgtgagacgc ttttcggcga
     1321 ttctgacagg acaccgtagc aaagccgcgc cctcagccca accttctcgc cgtcggggcg
     1381 agggcgatcg attggccttg tggccggcga aaccgggttg acccgggttg gctggacctc
     1441 tccccgttga ggggaaaggt ccgccacagg ccggttgagg gggcaaaccg ccgggattca
     1501 cgacgttcac gattccaagc ggatattgac gggctcaggt acccatctcc cagctttcga
     1561 gatacttctt ctgctcactc gtcaaggtgt cgatcgagat acccatggtc ttgagcttga
     1621 gggcggcaac ccattcgtcg acttccttgg gcacctggta caccttgtgc tcgagcttgc
     1681 cc
//
LOCUS       DATAQL010001350         3611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1207668, whole genome shotgun sequence.
ACCESSION   DATAQL010001350 DATAQL010000000
VERSION     DATAQL010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207668"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHS97_23235"
     CDS             <1..199
                     /locus_tag="VHS97_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451186.1"
                     /translation="LEDHGCAVDDPLVRAWWNDTGFVGRCPHCDGWIHFTIRSKRAIT
                     PDEASQHPQLPDDWHARATIL"
     gene            348..1847
                     /locus_tag="VHS97_23240"
     CDS             348..1847
                     /locus_tag="VHS97_23240"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3451187.1"
                     /translation="MKRAFLCLIGVLALAVGAVPLRAVEDVKPNIVFFLADDMGFMDI
                     GANNPRTFYETPNIDRLASSGMRFTEGHSSCPVCSPSRASLMTGKYPPRTGVTDYISR
                     GRYTQGKLTAAPNQDHIALEEVTIAERLRAGGYATFLAGKWHLGDGVFSPNAQGFGPG
                     LLTGKAGMFYYPPSDKPAPDPKVDPHTSDRIADEAVSFIYRHRDQPFFAYLPFLDVHT
                     PLQAPADLVAKYERKKSAAPPDSWGQEGERKVRLVQNHAIYAAMVEQLDRAVGRVVTA
                     IERAGLASRTIVIFTSDNGGLSTSEGHPTSNVPLRAGKGWPYEGGIRVPLIISAPGVT
                     RPGSLCQTPVITTDFYPTLLELTSLAPAPEQHLDGVSIVPLLRGGEILARTLFWHYPH
                     YGNQGGAPCGFVRDGDWKLIEWYEDGRRELYNLRDDPREHRDLAATNPDRLSNLTARL
                     AAWRHEVNAVMPTPNLQWDPKSQTKGPAANYSTIISPFMTIQWPGKVQR"
     gene            complement(1787..>3611)
                     /locus_tag="VHS97_23245"
     CDS             complement(1787..>3611)
                     /locus_tag="VHS97_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX3451188.1"
                     /translation="WPQVITTKGKPGQTGSWPYVVDTIEPPFRNPWNALLFFGDLDFF
                     ADGTAMLATVQGDVWRVDGLDEKLDQVRWRRFAMGLHQALGVAVAENKVYVLGRDQIT
                     CLHDLNDDGEADFYECFSNSYPTSTGGHDFISGLQRDGSGRFYTASSKDGLLRIAADG
                     RSFEKVATGFRNPDGLGLAPDGTMTVPNSEGEWVPTSMICEVRPGGHYGYPGPKAGQP
                     PDLPLVYLPRGIDNSSGAQVTVPDGRFGPLQGQLLHLSYGTGSHFLVLRDKVDGQPQG
                     AGVLLPGEFLSGAHRGRFNPKDGQLYVAGMAGWGTYTPADGCFQRVRYTGGPVQLPVA
                     WHAHENGVALTFSRPLTREVAGHANRHFAQAWNYHYSASYGSQELSIRHPGQPGHDPL
                     AIRSAHVLADGQTLFLEIPDLQPVNQLHLHVSPGAGEPIDLYATVHRLAAPFTGFPGY
                     LPSQKTIGAHPILADMEALKHPPAPNPWKGRIRGERAVSIDAGKNLSYSVRSFKVKTG
                     EPIRLTFVNPDSVPHNWALIKPGTLNRVGDLVNKIIAEPDAASRQYIPRTSDVLVYTD
                     IVVPQEQFAISFRAPTAPGRYPYLCTFPGHWMVMNGEMIVE"
BASE COUNT          732 a         1142 c         1065 g          672 t
ORIGIN      
        1 cttggaggat cacggatgtg ccgtcgatga ccccttggtg cgcgcctggt ggaacgacac
       61 cggctttgtt ggccgatgtc ctcactgcga cggctggatc cacttcacca tccgatcgaa
      121 gcgcgccatc acacctgacg aggcttccca gcatccgcaa ctgccggacg actggcatgc
      181 ccgcgcgacc atcctttagc ggtcaatagc cgcgccgcga agaacacgcg cccgccctga
      241 cgagcacgac accatcctcg cccgaaataa aaccaacctg cgatcgaaac atgtccagag
      301 atcgggacaa cgcgccacag cgccggccgt tgtttgcagg ggcaatcatg aaacgtgcat
      361 tcttatgtct gatcggtgtc ttagcacttg ccgtaggcgc agtccccctg cgcgcggtcg
      421 aagacgtgaa gcccaatatc gtcttctttc tcgccgatga catgggcttc atggatatcg
      481 gcgccaataa tcctcgaact ttttatgaga cgccgaatat cgaccgcctg gcctccagcg
      541 gcatgcggtt caccgaaggt cactcaagtt gcccggtgtg ctccccttcg cgcgcgagcc
      601 tgatgactgg caagtatccc ccccgaaccg gagtgaccga ttacatctcc cggggaagat
      661 acacccaggg caagctgaca gccgctccca accaggacca tattgccctc gaagaagtta
      721 caatcgcaga gcgcctgcgg gccggcggct acgcgacttt cctcgcgggt aaatggcacc
      781 tcggcgatgg cgtattttcg cccaacgcac agggctttgg accagggctg ctcaccggca
      841 aggccggcat gttttactat ccgcccagcg acaagccagc gcctgatccc aaggtcgacc
      901 cgcacacctc cgaccgtatc gccgatgaag ccgtcagttt catctaccga catcgcgacc
      961 aaccgttctt cgcgtatctc ccgttcctgg acgttcacac gcccctccag gcgcccgccg
     1021 acctcgtcgc caaatatgag cgaaagaaat ccgctgcgcc gcccgattcg tggggtcagg
     1081 aaggtgagcg caaggtgcgt ctcgtacaga accatgccat ctatgcggcc atggtcgaac
     1141 agttggacag ggcggtcgga agggtggtta ccgcgatcga gcgcgctggg ttggcctcgc
     1201 ggacgatcgt cattttcaca tcggacaacg gcggactctc gacttccgaa ggacacccca
     1261 cctcgaatgt acctctgcgt gctggcaaag gatggcctta tgaaggaggc attcgtgtcc
     1321 cgttgattat cagcgcgccg ggcgtcactc gccccggaag tctgtgtcaa acacctgtca
     1381 tcacaaccga cttctatccg acacttctcg agctgaccag cctggcgccc gcgcccgagc
     1441 aacaccttga cggtgtcagc atcgtcccgt tgctcagagg aggcgagatt ctcgcgcgca
     1501 ccctcttctg gcattaccct cattatggca accaaggggg tgccccgtgc gggttcgtgc
     1561 gggacggcga ctggaagcta atcgaatggt acgaagacgg tcgtcgcgag ctttataacc
     1621 tgcgcgacga tccccgcgaa caccgcgatc ttgccgcgac gaatccggac aggctcagca
     1681 acctcacggc gcgattagct gcctggcgcc acgaggtcaa cgccgtgatg cccacgccga
     1741 atctccagtg ggatcccaag agccagacga aagggccggc cgcaaactac tccacaatca
     1801 tctccccatt catgaccatc caatggcctg ggaaggtgca gagataggga taacgcccag
     1861 gcgcagtggg ggcgcgaaac gagatcgcga actgttcttg cggcacgacg atgtcggtgt
     1921 ataccaagac gtcgctcgtc ctgggaatgt actggcgcga ggcagcgtcg ggctcggcga
     1981 tgatcttgtt caccaggtca ccgacgcggt tcagtgtccc aggcttgatc aaagcccaat
     2041 tgtggggcac cgagtcgggg ttcacaaacg tcagccggat tggctcgccc gtcttaacct
     2101 tgaatgagcg caccgagtaa ctcaggtttt tgccggcgtc aatcgagacg gctcgctcgc
     2161 cgcggatacg tcctttccag gggttgggag ccggcggatg ctttagcgcc tccatatcag
     2221 ccaggatcgg atgagcgcct atcgttttct gggacggcag gtagccggga aatcccgtga
     2281 acggcgcagc caggcgatgc acagtcgcat ataggtcaat gggttcgccc gcacccgggc
     2341 tgacgtgcaa atgaagctga ttgaccggct gaagatccgg gatctcgaga aaaagagtct
     2401 gtccgtcggc caggacgtgc gcggaccgga tcgccagggg gtcgtggccc ggctgaccgg
     2461 gatgtcgaat cgaaagttcc tgagagccgt agctggcgct atagtgatag ttccacgcct
     2521 gagcgaaatg acgattcgcg tggccggcga cttcacgggt caacggtcga gaaaacgtta
     2581 gcgcgacccc gttttcgtgg gcgtgccatg caactggcaa ttgaaccggg ccgccggtgt
     2641 agcgcacgcg ttgaaaacag ccgtcggccg gcgtatacgt gccccatccg gccatacccg
     2701 cgacatagag ctgcccatcc ttgggattaa atcgtccgcg gtgggctccc gagaggaact
     2761 cgccgggcag aagcacaccg gccccttgcg gctggccgtc gaccttatcc cgcaagacca
     2821 ggaagtggct tcccgtcccg taggagagat ggagcaactg tccctggagc ggcccaaatc
     2881 tgccgtcggg caccgtgacc tgggcaccgc tggaattatc aattccgcgc ggcaggtata
     2941 ccaaagggag atcgggcggt tggccggctt tgggacctgg ataaccataa tggccgccgg
     3001 gtcgaacctc gcagatcatc gatgtcggga cccattcccc ttcggaattg gggacggtca
     3061 ttgtgccatc aggcgccagc ccgagaccgt caggattacg aaacccggtc gcgaccttct
     3121 cgaacgagcg gccatcggct gctatccgca gcagtccatc cttgctcgac gcggtgtaaa
     3181 agcggcctga tccgtcgcgc tgcaggccac ttatgaagtc atggccgccc gtggaggttg
     3241 gatacgagtt cgagaagcac tcgtaaaaat cagcttcgcc gtcgtcgttc aggtcgtgga
     3301 ggcacgtgat ctggtcgcgc cccagcacgt ataccttgtt ttcggcgacc gccacgccca
     3361 gagcttggtg caaacccatt gcaaaccgcc gccaccgcac ctgatcgagt ttttcatcga
     3421 gaccgtcgac tcgccaaaca tccccctgca cggtcgcgag catcgccgtg ccgtcggcga
     3481 agaagtccag gtcgccgaag aacaggagcg cattccacgg attgcgaaac gggggctcaa
     3541 tggtgtcgac cacgtagggc cacgatccgg tctggcccgg ctttcccttg gtcgtgatga
     3601 cctgcggcca c
//
LOCUS       DATAQL010001351         9369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5176199, whole genome shotgun sequence.
ACCESSION   DATAQL010001351 DATAQL010000000
VERSION     DATAQL010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5176199"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="VHS97_23250"
     CDS             complement(<1..2001)
                     /locus_tag="VHS97_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX3451189.1"
                     /translation="MGRVNRRRQQTERLNVMVVNACVVLAMGSAILACSNAGLAAEPR
                     KPAVKDSSKAIKPKTAVLTASVEPAEARPGDTVTFKVTAKVDPGYHIYKYSKTKVAGG
                     PVNTSFDFFDPADLEVKGDWTPSTEPEKHKDPNFPELDSVDYHEDEVTWSIKLKIPES
                     TAPGKKVLHCQAGYMVCDAKHCSIPGLWTLPETVLTVLPGDGKGPKAAVTAPDTQPKT
                     PAPTPANQKSEMATSDLPKNSTPSDTKPAASLPSAPVVQSEIAQKAQQGLIPFLIASA
                     IGGLFALVMPCVWPMVPITVNFFVKQGQGKGGRNKATGLAIIYCLSIIGIFTAVGVLF
                     SFFFSASSLQTLANNPWLNLFVAGLFVAFGLSLLGLFELRLPNFLLNASAQGESKGGL
                     VGVVFMALTLTITSFTCTFPVVGGLLVMAAGGDFFYPVVGLATFATVIALPFFLLALS
                     PGLISKMPRSGDWMNAVKVVGGLVEIGAAFKFVNTAELAWVTPENAWFDAQVVLTSWV
                     VLSVVCGIYLLGLFRTDHDHDEVKVGPGRIMCGSLFLALALFMAPALFGRPPASLVWD
                     RLIVGILPPDSNELSADVQIAGKANESAGSGSLKEVKATSTDPAKAEREEKKAHGVLW
                     GMSFDQARELAATEKKPVLIDFTGVNCANCRLMERRVLPRPEV"
     gene            2361..2990
                     /locus_tag="VHS97_23255"
     CDS             2361..2990
                     /locus_tag="VHS97_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEX3451190.1"
                     /translation="MRKALHGLILLIAAAGALWLIVLDRRGRLVWDHWDEAKRDVLYR
                     SGQLTGDQLTAAIKRHNIRTVINLQLPGDSMMAERALTARLGVNFVNLPMPGDGFGEE
                     WQFREILKITDDPQRRPVLVHCARGTCRTGAAVAIYRLERDGWLLEDVAAEMRRQTYR
                     YGWLPGYIYAMAKNKPALAGPRPEMIDDRNQVADSSPQGLAAIGQGGQP"
     gene            2987..4666
                     /locus_tag="VHS97_23260"
     CDS             2987..4666
                     /locus_tag="VHS97_23260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451191.1"
                     /translation="MSLDPTLIKTVHRFKFKTLRHDRAATWIPPALNRTGVWLGMAGL
                     TALFVVLGVINLDLGSPEAKLGLAAGEMLGPVGQVFGYWAPDLWPARVFPSLVLGRLE
                     AGGRPSPAAVRWPEAIAAIVVGWMIARRMCRQFGLRAGLLFGVCWFGGLALIDRSAGT
                     GIDWIMGLAAVAAIDRLIAQGSDWVAGLWAALAFLAGGWPPLVVIALAVIVVGKPISK
                     FSLGLVLPPVLTSIAWSVWTIQACSVELWAAALSRPFTRKPDWSFCLSVLALGLPWSP
                     FSLLALFRSVRESWRPEGRSWLNSWLLVTVASSIGATIVPGVSPAARLVVLAGLMMVT
                     TLCLESAWARTLTDSSRLAFFIAFGCVFGAWLAVMLYGSYVWNLAMPFYRPLGIIMTI
                     MALGVGVLGMSALETRNSRRGLVTLMVIAVGLKLAHWGYYVPEWNYRYSQGPWGRAIG
                     QWIPKKWVVYTFHDWQPDLAFFVKHPVRQLRSPHYLEYEGGHESKFVLLQDSEYENWP
                     DSALPVTLVAKFLDQSARERVLARTAGPLPPPLGPNPLSFNASRDNGVASE"
     gene            4972..5919
                     /locus_tag="VHS97_23265"
     CDS             4972..5919
                     /locus_tag="VHS97_23265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3451192.1"
                     /translation="MARTVPKVQDNSECAPMLGKMMDFFGSGTSTSSKGPRRRENLQR
                     RFSIVSETSQGSMSRVYRAIDNLTARTVCLKVQSREKNEAAKLRASVSEIRPPEGEMA
                     IQFVHPHIVRTLEYGTSTQGEHYLVMEYIDGVSIQYVRESRSARTAQKVELLAQAAEG
                     LAAVHASGFIHHDINPRNFLVDRDQNVKLIDFGLTVPNAPAFRRPGNRTGTVHYMAPE
                     LLRREPIDERIDIFAFGVLAFEFLTDRLPYDATNTTTMMLQRINSEPLNPAAVKPKLS
                     DELCNILRQLTARRKDERWPRMCTLADALRSIPVKRQRV"
     gene            complement(6053..6967)
                     /gene="prmC"
                     /locus_tag="VHS97_23270"
     CDS             complement(6053..6967)
                     /gene="prmC"
                     /locus_tag="VHS97_23270"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX3451193.1"
                     /translation="MNGRQHPTPQPSPLTPPEDPWSVGRLLTWTTDYLKRHGSESARL
                     DAEVMLAHVLEWQRVQLYTHFEEEISQAARTAFRELVRGRALGTPVAYLVGRKEFYSL
                     SFAVSPAVLIPRPESEFVVVEYLEVTRHLPTASAVDVGTGSGCLAIASAHHHQGSRFV
                     AIDVSEPALAVARRNAAEIGVANRIDFRHGDRLGPVTGDGPFDAIVSNPPYIPSESIE
                     HLEPGVRDHEPTIALDGGPGGLDMVARLIEESVGHLKPGGHLILEIGTGQEGPVRSLI
                     MAQPGLRLAPTVFDHSNHPRVIRATRTD"
     gene            complement(7041..8108)
                     /gene="prfA"
                     /locus_tag="VHS97_23275"
     CDS             complement(7041..8108)
                     /gene="prfA"
                     /locus_tag="VHS97_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX3451194.1"
                     /translation="MFEKLQADYQRFVEIEQQLLDPEIVTDPNKATTLAKERGTLAKI
                     AIPYGRYLELSRQLDEAEQLLASETDAEMRSYAEAELETLRARHEQDGEVLRDMLYDR
                     TAGTGHTALIVEIRAGTGGGEAALFARDLYEMYRRFAERMRWKFEILDLEATELGGFR
                     EVSFSVAGEAAFRHLQFESGVHRVQRVPETEAQGRLHTSTATVAVLPEPEDVDIVIRT
                     EDLQIDVMRSGGPGGQHQNKTESGVRITHLPSGIVVNCRDERSQHKNKAKAMRILRSR
                     LFDQIQESARSHRDQTRRDLIGSGDRSQKMRTYNFPQNRLTDHRIGLTLYNLNQVVQG
                     DLLALTKALIDHDRREQLGDL"
     gene            complement(8462..8704)
                     /gene="rpmE"
                     /locus_tag="VHS97_23280"
     CDS             complement(8462..8704)
                     /gene="rpmE"
                     /locus_tag="VHS97_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX3451195.1"
                     /translation="MKDGIHPKYQECVVTCGCGNSFKTRSTKAKIMVEVCSKCHPYFT
                     GSVKFVDAAGRVDKFNKKFQGTYGKAKKATATADKT"
     gene            complement(8755..>9369)
                     /locus_tag="VHS97_23285"
     CDS             complement(8755..>9369)
                     /locus_tag="VHS97_23285"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3451196.1"
                     /translation="LDSNHSGYDHDHALPIFSRQKMRSGFTVLAATILTAGLAIVCVA
                     RTACSDESSSADAVRLDRLTWKQLQERLVNNKVKFTIVDAWSTTCGPCKENFPHVVEM
                     HRKYASKGLAVISLSLDDPTDTAAVAEAEKFLKAKKAACTNVLLDETFGDGFEKLKIN
                     GIPAVFVFGPDGKEVKRFTMDDPNNQFTYDEVEKAVVALLDGKQ"
BASE COUNT         1758 a         2786 c         2812 g         2013 t
ORIGIN      
        1 aacctcgggc cggggcaaga ctcgccgctc catcagccgg caattcgcgc agttgacgcc
       61 ggtgaagtca atcagtaccg gcttcttctc agtggcggca agctcacggg cctggtcaaa
      121 gctcatgccc cagagcacac cgtgagcttt cttttcctcg cgctcggctt tggcgggatc
      181 agtcgatgtc gccttgacct ctttcaacga gcccgagcca gcggactcat tggctttgcc
      241 cgcaatctga acgtctgcgg acagctcgtt ggaatctggc ggcaggatgc cgacgatcaa
      301 acgatcccag acaaggctcg ccggcggccg gccgaatagc gcgggcgcca tgaaaagcgc
      361 cagcgccaga aagagagatc cacacatgat tcgcccgggc cctaccttga cttcgtcgtg
      421 gtcatggtcg gtgcggaaaa gtccgagcag gtagatgccg catacgaccg acagcacaac
      481 ccagctcgtc aagaccacct gggcatcgaa ccacgcgttc tcgggagtga cccaggccag
      541 ctcggccgtg ttgacgaact tgaaggcggc gccgatctcg accaagcccc ccacgacctt
      601 gaccgcgttc atccagtcgc cgctccgggg catcttggag atcaatcctg gagacagggc
      661 aagcaggaag aacggcaagg caattacggt ggcaaaagtc gccagcccga ccaccggata
      721 aaagaagtca cctccagccg ccatcacgag cagcccgccg acgaccggga aggtgcaggt
      781 gaacgatgta attgtcaggg tgagtgccat gaagaccaca ccgacaaggc ctcccttgct
      841 ttctccttga gcggaggcgt tcaagaggaa gttcggcagg cgcagttcga acaatcccag
      901 cagactgagc ccgaacgcca cgaagaggcc ggccacgaac aggttcagcc acggattgtt
      961 ggccaaggtc tgaagggaag acgcggagaa aaagaacgag aagaggaccc cgaccgccgt
     1021 gaagatcccg atgatcgaca ggcaatagat gatggccagc ccggtcgcct tgttccgccc
     1081 tccctttccc tgtccttgct tgacgaaaaa gttgacagtg atcggcacca tcggccacac
     1141 gcagggcatc accagcgcga acaggccgcc gatcgccgag gcgatcagaa agggaatcaa
     1201 gccttgctgg gccttctggg caatttcgct ttgaacgacg ggcgctgaag gcagcgaggc
     1261 tgctggcttc gtgtccgagg gagtggagtt cttgggcagg tcgctcgtcg ccatttcgga
     1321 tttttggttc gccggcgtcg gtgcgggggt tttcggctgg gtatctggcg cggtcacggc
     1381 ggccttgggg cctttgccgt cgccgggcaa gaccgtcagc accgtttcag gaagggtcca
     1441 caggcccggg atgctgcagt gcttggcgtc gcagaccatg taaccagcct ggcagtgtaa
     1501 aactttctta ccgggcgcgg tactttccgg aatcttgagc ttgatgctcc aggtgacctc
     1561 atcttcgtga tagtccaccg aatcgagctc ggggaaattg ggatccttgt gcttctcggg
     1621 ctcggtggag ggggtccagt ctcccttgac ctcgaggtcc gctgggtcga agaagtcgaa
     1681 ggaggtattg accggtcccc ctgctacctt cgtcttcgaa tacttgtaaa tgtgatagcc
     1741 cggatcgact ttggctgtca ccttgaacgt tacggtatca cccggtctgg cctccgccgg
     1801 ctcgaccgac gctgtgagaa ccgcagtctt cggcttgatc gctttcgagg aatctttgac
     1861 ggccggcttg cgaggctcgg ctgccaaccc cgcgttcgag cacgccaaaa tcgcggagcc
     1921 catcgccagc accacgcatg catttacaac catgacgttg aggcgctctg tttgctgcct
     1981 acgacgattg actcgaccca tgattagatt tcccatacgg attgggacgt gaaacaacgc
     2041 tctcgggagc gccaaaaata catcttcatc ctagagcctg ggacgaagac ggacaagccg
     2101 atcgaacgcc gtagctcgga cgttcctttc agcaaatccc ttggtctcgg ccagaatgtg
     2161 atcgcagtcg ggcctggttc ggattgacca ttccgccaat ctagcctacg attccccgcc
     2221 gtgaagaggc gatcctcacc ttgtgcattt cttcctcgct cgcttctcat cctcaatccg
     2281 agccgcgcct cgcccagaaa tgtcggccgc gcggtttccc ggcacgggtc aggtccccgt
     2341 gtcagcaagg actttgctcg atgcgcaagg cgctccacgg tctgatcctg ctcatcgccg
     2401 cggccggcgc cctttggctg atcgtgctgg atcgacgagg caggctggtc tgggaccact
     2461 gggatgaggc aaagagagat gtcctttatc gcagtggcca gttaactggc gatcagctta
     2521 cggccgcgat caagcgacac aacattcgta ccgtcatcaa cttacaatta cctggcgaca
     2581 gcatgatggc cgagcgtgcc ctgacggctc gtctcggcgt caatttcgta aacctgccga
     2641 tgccaggcga tggttttggc gaagagtggc aatttcgcga gattctcaag atcaccgacg
     2701 atcctcagcg acgccccgtg cttgtccact gcgcccgcgg aacatgccgc accggtgccg
     2761 ccgtggctat ctaccggctt gagcgtgatg gctggttgct cgaagacgtt gccgccgaga
     2821 tgcgtcggca aacctatcga tacggctggc tcccgggtta catctacgct atggccaaga
     2881 acaagccggc attggccggc cctcgtcccg agatgatcga cgaccgcaac caggtggcgg
     2941 actcgtcgcc ccaaggcctc gcggcgattg ggcaaggagg ccagccatga gccttgaccc
     3001 gaccttgatc aagacggttc atcgtttcaa gttcaagaca ttgcggcatg atcgagccgc
     3061 gacatggata ccgccggcac tgaaccgaac cggggtatgg ttgggtatgg ccgggctgac
     3121 cgctttgttt gtggttctgg gtgtaatcaa tcttgatctc ggctcacccg aagcaaaact
     3181 cggattggcg gcgggcgaaa tgctggggcc cgtgggccag gtctttggct actgggcgcc
     3241 cgatctctgg ccggctcggg tctttcccag cctggtgctc gggaggctgg aggcgggagg
     3301 ccggcccagc ccggccgcgg ttcgctggcc ggaagcgatt gccgccatcg tcgtgggatg
     3361 gatgatcgcg cgacgcatgt gccgccaatt tggtttgcgc gctggtcttc tgttcggtgt
     3421 ttgctggttc ggcggcctgg ctttgatcga tcgctccgcc ggtacgggca tcgactggat
     3481 catgggattg gccgcagttg cggcgatcga ccgtctcatc gcgcagggat cggattgggt
     3541 cgccggcctg tgggcagccc tggcgttcct ggcgggagga tggcctccgc tggtagtgat
     3601 cgccctggca gttatcgtcg tcggtaaacc gatctcaaaa ttctctctcg gtctggtgtt
     3661 gccgcccgtc ttgacgtcaa ttgcgtggtc ggtttggacg attcaggcgt gttcggtcga
     3721 gctctgggcg gccgccctca gccgtccctt cactcggaag cccgattggt cattctgcct
     3781 ctctgtgctg gctctgggct taccctggag cccgttttcg ttgctggccc tcttccggtc
     3841 ggtacgtgag agttggaggc cggaaggtcg gtcttggttg aacagttggt tactcgttac
     3901 cgtggcgtcg tcgatcggcg cgaccatcgt gccgggtgta agtcccgccg cgcggttggt
     3961 cgtgctggcc gggctgatga tggttacgac cctctgcctc gaatcggcct gggcgcgtac
     4021 actgaccgat tcgtcgcgtc tcgcattctt catcgcattc ggctgtgtgt ttggtgcttg
     4081 gttggccgta atgctttatg gcagttacgt ctggaacctg gcgatgccgt tttatcggcc
     4141 gctgggaatc atcatgacca tcatggcact cggggtcggc gttctgggta tgtcggcttt
     4201 ggagacgcgc aattcccggc gcgggctggt tactttgatg gtcattgcgg tcggcttgaa
     4261 gctggctcat tggggctatt acgtccccga atggaactac cggtacagcc agggtccgtg
     4321 gggccgagcg atcggccagt ggatccccaa gaaatgggtc gtgtacacgt tccacgactg
     4381 gcagcctgac ctggccttct tcgtcaaaca tcccgttcgc caattgcgca gcccgcacta
     4441 tctggaatac gagggcggtc acgagagcaa atttgtgttg cttcaagatt ccgaatatga
     4501 aaactggccc gattccgccc tgccggtgac cctggtcgca aaatttttgg accaatcggc
     4561 gagggaacga gtcctggcgc gcactgctgg cccactccct cctccgctcg gaccgaatcc
     4621 cctgagtttt aacgcctcgc gtgataacgg tgtcgcgtcc gagtaaattc ggacttgccc
     4681 gccgacagcg tcccctagcc cacatttcca caacgaagcg cgcgaaactt gccttgaact
     4741 tatgtcactt catcgcacat ccgtcgtatg attgctgagg gatccaacgc aatggccatg
     4801 cgcgccgggg aaattgccac gcgtcgcgag cgaatttagc tgagtgacct gagagatctg
     4861 tcggtccatg gtgactgatc ctgatagatc gcagggtacg aggtttgccc agtcgaatcc
     4921 gctatcactg atgcgtgcgt gaattatcag cgtccgatat aacaagaagt gatggcacga
     4981 acggttccga aggttcaaga caacagcgag tgtgccccga tgctcggcaa gatgatggat
     5041 ttttttggtt ccggcacgtc gacctcgtcg aagggtcctc ggcgccgcga gaacttgcag
     5101 cgccggtttt cgattgtgtc ggagacgtcg caggggagca tgtcacgcgt ctaccgtgcg
     5161 atcgacaatc tgaccgcccg caccgtgtgc ctgaaggtcc agtcccgcga aaagaacgag
     5221 gcggcaaagt tgcgggcgtc ggtgagtgag atccgtccgc ccgaaggcga gatggcgata
     5281 caatttgtgc atccgcacat cgttcgcacc ctcgagtatg gcacctcgac tcagggagag
     5341 cattacctgg tgatggagta catcgacggc gtgagcattc agtatgttcg cgagtcgcgc
     5401 tcggcccgaa ccgcccagaa ggtcgagttg cttgcccagg cggcagaggg acttgcggcc
     5461 gtacatgcga gcggttttat ccatcatgac atcaaccccc gtaatttcct ggtcgaccgc
     5521 gatcagaacg tgaagttgat cgacttcggt cttaccgttc ccaacgcgcc cgcgtttcgg
     5581 cgcccaggga atcgcaccgg cacggtgcac tatatggctc ccgagttgct gaggcgcgag
     5641 ccgatcgacg agcgcatcga tatctttgcg ttcggtgtct tggccttcga gttcctgacc
     5701 gatcgccttc cctacgacgc cacgaatacc accaccatga tgctccagcg aatcaacagc
     5761 gaaccgctca acccggccgc tgttaaaccc aagctctcgg atgagctgtg taatatcctc
     5821 cgccaactga cagcgaggcg caaagacgag cgctggccgc gtatgtgtac gctggcagac
     5881 gcgttgcgct cgatacccgt caagcgacaa cgggtttagg cgaccggcga gtgcaagatt
     5941 gcccaggtag ggtgggcacc gcccaccacc cggggccccg ttctacaaga cggggtacgt
     6001 aattcctgcc ggtggcctta atcggcgggc gcgatcggcg ggaggaactc gctcagtccg
     6061 tgcgggtcgc ccggatgacg cggggatggt tggaatggtc aaacacggtc ggggccaacc
     6121 tcagcccggg ctgcgccatg attaatgagc gaaccggacc ttcttgtccc gtgccgattt
     6181 ccaggatcaa gtgaccgccc ggtttcagat ggccaaccga ttcctcgatc agccgggcaa
     6241 ccatgtccag tcctcccggg ccaccgtcga gcgcgatcgt tggttcatga tcacggactc
     6301 ccggctcgag atgctcgatt gattcgctag gtatgtaagg ggggttcgaa acgatcgcgt
     6361 cgaacgggcc atcgccggta accggtccca gacgatcgcc gtgtcgaaaa tcgatccggt
     6421 ttgcaacgcc aatctcggcg gcattccgcc gcgcgaccgc cagcgccggc tccgagacgt
     6481 caatggcgac gaaccgactt ccttgatggt ggtgagccga ggcgatagcc agacagcccg
     6541 agccggttcc gacatcgacg gcagaggccg tcggtaggtg gcgggtgacc tcaagatact
     6601 cgacgaccac aaactccgat tccgggcgcg gtatcagcac ggccggcgag actgcgaagc
     6661 tcagcgaata aaactccttg cgtcccacga gataggccac cggcgtcccg agcgcacggc
     6721 cgcgaaccag ctcgcgaaat gcggtgcggg cagcctggct gatctcttcc tcgaagtgag
     6781 tgtagagctg cacgcgctgc cactccaaga catgcgccag catgacctcg gcgtcgagtc
     6841 gcgcgctttc cgacccatgg cgcttcagat aatcggtggt ccacgtcaac agcctcccca
     6901 cactccaggg atcctcggga ggcgttagag gactcggttg cggcgtcggg tgctggcgtc
     6961 cgttcatcgc gggcaataag acctcactcc attgtcagcc gtcgagtcgg ccttcctttc
     7021 gcgcatcacc ggacctggta ctacaggtca ccgagctgct cacggcgatc gtggtcgatc
     7081 agggctttgg tcagcgcaag cagatcgcct tgtacgacct ggttgagatt ataaagggtc
     7141 aagccgattc gatggtcggt caaccggttt tgcgggaagt tgtaggtacg catcttctga
     7201 gagcgatccc ccgatccaat caagtcgcgg cgagtctggt cgcggtgaga gcgcgcactt
     7261 tcctgaatct ggtcgaaaag ccggctccga agaatccgca tggccttcgc tttgtttttg
     7321 tgctggcttc tctcgtcgcg gcaattgacg acgatgcccg agggaagatg ggtgatccgt
     7381 acgccgcttt ccgttttgtt ctggtgctgc ccgcccggcc ctcccgaccg catgacgtca
     7441 atttggaggt cttcggtccg aatcacgata tcgacgtctt cgggctcggg caagacagcg
     7501 accgtcgcgg ttgacgtgtg cagccgccct tgggcttcgg tttcgggcac gcgctgaacg
     7561 cggtgcacgc cactttcgaa ttgtaaatgt cggaaggccg cctcgcccgc cacactgaac
     7621 gacacttccc ggaagccgcc cagttcggtc gcctcgaggt ccaggatctc gaacttccag
     7681 cgcatgcgct cggcgaaccg ccggtacatt tcgtagaggt cgcgcgcgaa caacgctgcc
     7741 tcgccgcctc ctgtccccgc ccggatctca acgatgaggg cagtatgacc cgttccggcg
     7801 gtgcggtcgt acagcatgtc acgcaagact tctccgtctt gctcatgccg tgcccgcaag
     7861 gtctccagct cggcttcggc ataggaacgc atctcggcgt ccgtctcgct cgcgaggagc
     7921 tgttcggctt cgtcaagctg acggctaagt tcgagatagc ggccataagg aatcgcgatc
     7981 ttcgccagtg tgccacgctc cttggcgagt gtcgttgcct tattcggatc cgtgacgatc
     8041 tcgggatcga gcaattgctg ctcgatctcg acaaatcgtt ggtaatcagc ctggagcttc
     8101 tcgaacacgg aagaggcctc gatattctgg agtcgctgtc tcgagccagg aattttttcg
     8161 gagccgctgg tcccttaagc cgcgtaagta ctttccccca cttgtgatgg gggggttggg
     8221 gggtggtcag ggcgctatca gacacaaggc ttcggataaa gttgccacct gatcggcgtt
     8281 cgttccctcg ttcttgatga gtttgaatcg ctgcttctga ttgcgccggg accaaccccc
     8341 caaaccccca cttcacaagg cggggaaagc tcggtcgccc gttctctgac aacgcagaac
     8401 ggcccgcgac cgaagctggc gctccggtcg cgggccgtca tcgcaatttc gtagttcccg
     8461 gctaggtctt gtcggctgtg gccgtcgctt tcttcgcttt gccgtaagtt ccctgaaatt
     8521 tcttgttgaa cttatcgaca cgtccggccg cgtcgacgaa cttcaccgat ccagtgaaat
     8581 acggatggca cttcgagcat acctcgacca tgatcttggc cttggtacta cgggtcttga
     8641 agctattgcc acatccgcag gtgacaacgc attcctgata tttgggatgg attccgtctt
     8701 tcattgcttt cgagctcggt caagaggtcc agttggtctc gcttcctttt tatcttactg
     8761 tttgccgtca aggagtgcga ccacggcctt ttcgacttca tcgtaggtga actggttgtt
     8821 ggggtcgtcc atggtgaatc gcttcacctc tttgccgtcg ggtccaaaca caaagaccgc
     8881 ggggatcccg ttgatcttca gcttttcgaa cccgtcgcca aaagtttcgt cgaggagaac
     8941 gttggtgcaa gcggcctttt tcgctttgag aaacttctct gcctcggcga cggccgccgt
     9001 gtcggtaggg tcatcgagcg agagggagat cacggccaag cccttgctgg cgtacttgcg
     9061 gtgcatttca acgacatgag gaaaattctc cttacaaggt ccacaggtgg tggaccatgc
     9121 gtcgacaatc gtgaacttga ctttgttgtt caccaagcgt tcttgtaact gtttccatgt
     9181 aagccggtcg agcctgacgg catccgctga ggagctctcg tctgagcagg cggttctggc
     9241 aacacacacg atggccaatc cggccgtaag aattgtcgcg gcgagaacag taaatccaga
     9301 tcgcattttt tgcctcgaaa agattggcaa cgcgtggtcg tggtcataac ccgaatgatt
     9361 cgagtcaag
//
LOCUS       DATAQL010001352         4500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4717036, whole genome shotgun sequence.
ACCESSION   DATAQL010001352 DATAQL010000000
VERSION     DATAQL010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4717036"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1442)
                     /locus_tag="VHS97_23290"
     CDS             complement(231..1442)
                     /locus_tag="VHS97_23290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451197.1"
                     /translation="MTAIQVVILVVYAVIVAAWPIRLFVLDVILRRQSALVSESPRYD
                     RPDPPLVSAILPAKDEEAYLALCLQSICKLTYPNLEILVVDDRSTDRTGEIARRIASG
                     DARVRVFTIDELPPGWTGKTHALEHASHLARGQWLLFVDADTIHAPDSLGIMMEFAHR
                     EQAVLASLLPELRCESFWERVMQPIAAITLMQSFPLHEVNNDWSKLAFANGQYILIER
                     TAYDAAGGHEAVRDRFVEDIALAQRVKALGLPIRVALARGIVSCRMYASFAQLVRGWS
                     RIFYDALGRNPWRLVKKLLDPIVFCQTGHVALGAGLVLLARGGGIFAVSLSAMSIAHH
                     AFMYLVFRRVYRAAAERPSFSSWYPIANLVVDLILIRAICMCLTGNVTWRGTNYAVSA
                     PAIELDAGSQK"
     gene            complement(1512..2078)
                     /locus_tag="VHS97_23295"
     CDS             complement(1512..2078)
                     /locus_tag="VHS97_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451198.1"
                     /translation="MPSEFWLVLTVAAAFLLLGIAGSPLAWVVLYHRRSRLEIAMDRK
                     YQYLFVQLKELDGRVLQLESAGSDDRRLQSPSAARVSMRTDSPAETIGRVKSPWWRTP
                     TVGANDPGEHALIAVPSLEPAHSDRGVTMNGLKERYAAIWALADKGATLEQMARATGQ
                     PIGQIELILGLRRQIDGPPTRIPHAPHT"
     gene            complement(2202..3407)
                     /gene="larC"
                     /locus_tag="VHS97_23300"
     CDS             complement(2202..3407)
                     /gene="larC"
                     /locus_tag="VHS97_23300"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX3451199.1"
                     /translation="MRLGYFDCFSGIAGDMTLAALVDAGVDRRPIQEAVASLGLPCEL
                     TFETVRRGGMRATYAKVTAPHEHVHRHWHHIEAIIDKSLLTSRQQDLAKRIFLKLGEA
                     EAKVHGVDLAKIHFHEVGAVDSIVDIVGSAVGLDLLGVDRYEASPVPTGRGWVKAAHG
                     RMPLPAPATAELLRGVPLALSMVDGEMTTPTGAAILTTVAERFTSLPAMTIEAIGLGA
                     GTREVPDQANILRLFVGDVNLPAASDRIWVLETNLDDLPGEIIGYTMTRLLEAGALDA
                     FVTPIQMKKNRPGVMVSVLCDEPKIPALESLLFRETSTLGIRRYQVSRHKLKRQPVKV
                     ETPYGAVQGKLGWLAERPPTFSPEYEDCARIAAAHGVPLREVYDAAHAAYAQQSRVTE
                     STGHEHNHT"
     gene            complement(3583..4404)
                     /locus_tag="VHS97_23305"
     CDS             complement(3583..4404)
                     /locus_tag="VHS97_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3451200.1"
                     /translation="MQDRYRNRYRVPVASDRVRRQIAREAARRLYSTLIPAGKDHPPN
                     WLDLAGPNDLYLAKRKAAAVLGHRIRPGDLPSDAEVREELVFLQRESSGDPDPLDTTP
                     EPAADTVPLSITDHLDRFAIYKMRLEPLETVKQNPKYHPEGDALFHSLQVFELARDER
                     PYDEEFLLAALLHDVGKAIDPQDHVAAGVEALRGAVTERTLWLIEHHMDLLSGREKPL
                     AARLKRELESSEYFEDLKLLRELDGAGRVPGQRVDSLDEVLAYLKWLEDESYLDQ"
BASE COUNT          920 a         1328 c         1374 g          878 t
ORIGIN      
        1 acgcttgccg tgatcagagc gattcgagcc ggcgcagaag accgatcgtg cgggaaagga
       61 gacccgtcct cttacttcag atcctcaaag cgaatcaacc tggggacatg acttcacctt
      121 ataagcgaag ttggctcgaa acgcaatcag cttgagaaga aaatcgtttt gacctgggaa
      181 aggcttctta tcgcgtctcg atgtaaggac tgaaggtgcc ggccagaggc tcatttctga
      241 cttccggcgt cgagctcgat cgcgggagcc gaaacggcgt agttggttcc gcgccaggtg
      301 acattgccgg tcaagcacat gcagatggct cgaatcaaga tcagatcgac gacgaggttt
      361 gcgatcgggt accacgacga aaaagaaggg cgctcggccg cggccctgta tacccggcgg
      421 aatacgaggt acatgaaggc gtgatgggcg atgctcatag ccgaaagaga gactgcaaag
      481 attccgccgc cccgggccaa gagcacgagg ccggcgccca atgccacatg tccggtctgg
      541 cagaagacga ttggatcgag gagttttttc acgagccgcc agggattccg accgagcgcg
      601 tcataaaaaa tgcggctcca acccctgaca agctgcgcaa aggaagcgta cattcggcac
      661 gacacaatgc cacgggccag ggccacacgg atcggcaagc cgagtgcctt gacacgctgg
      721 gccagcgcga tgtcttcgac aaaccggtca cgcactgcct catggccgcc ggctgcgtcg
      781 tatgccgtcc gctcgatcag aatgtactga ccgttggcaa aggccaattt agaccagtcg
      841 ttattgacct cgtgcagcgg gaacgactgc atgagggtga tcgccgcgat cggctgcatc
      901 acgcgttccc agaacgattc acaccgcagc tcagggagca agctggcgag gacggcctgc
      961 tccctgtggg cgaattccat catgattccc aagctgtccg gagcgtggat cgtatcggca
     1021 tccacaaaca aaagccactg cccgcgcgcc agatgagagg cgtgttcaag cgcgtgtgtc
     1081 ttgccggtcc agccgggagg cagttcgtcg atcgtgaaga cacgaacgcg cgcgtcgccg
     1141 gatgcaattc gtcgggcaat ctcaccagtc cggtcggtgc tccggtcgtc caccaccagg
     1201 atttccaggt tgggataagt cagtttgcag atcgattgca ggcacagggc caggtaggct
     1261 tcctcgtctt tggcgggcag gatcgccgat acgagtggcg ggtctggtcg atcgtagcgg
     1321 ggcgactcgg acacgagcgc actttggcgg cggaggatga catccagcac aaacagacga
     1381 attggccagg ccgcgacaat gaccgcgtaa acgacgagaa taacgacctg gattgccgtc
     1441 atggcgagga gccctcagcc tcttcgtcga ggaatctgcg tgtcgccgcg tcgaggcttt
     1501 ccgaactgtt ttcaggtatg aggtgcatgg gggatcctgg tcggcgggcc gtcaatttgg
     1561 cggcgcaggc cgagtatcaa ttcgatttga ccgatgggct ggccggttgc gcgggccatc
     1621 tgttcgagcg tggctccttt atcggcgagt gcccagatcg cggcataacg ctccttgaga
     1681 ccattcatag tgacgccgcg gtcgctatgt gccggctcca ggcttggaac cgcgatcaga
     1741 gcgtgctcgc ccggatcgtt tgcgccaaca gtcggcgttc gccaccaggg agatttcacg
     1801 cgacctatcg tttcggccgg cgagtctgtt cgcatcgaga ctcgagctgc ggacgggctc
     1861 tgtagacgcc ggtcatccga gccagcggat tcgagctgga gtacccggcc atcgagctct
     1921 tttaattgaa cgaacagata ctgatacttt cggtccatcg cgatttccag acgtgaccgg
     1981 cggtgataga gaacaaccca ggccagtggg gagccggcga tccccaagag caggaacgcg
     2041 gcggcgaccg tcaagacgag ccagaattcc gacggcatcg atcacatcct tccgtggatg
     2101 aaagcgggct catgggttct ctgcggttat ttctgggcgg cgggccgttt ggctttccgc
     2161 gtcgccctac cggcgtgagt cattgtgagt ttgggtcttg atcatgtgtg attatgctca
     2221 tgtccggtcg attccgtcac acggctctgc tgcgcgtagg cggcatgagc ggcgtcgtac
     2281 acctcccgca gcgggactcc gtgtgccgcc gcaatccggg cgcagtcttc gtattcaggg
     2341 ctaaatgtcg gaggccgctc cgccagccag ccgagttttc cctggacagc accatacggg
     2401 gtctcgacct tgacgggctg gcgtttcagc ttgtgccggc tgacctggta gcggcgaatt
     2461 cccaaggtcg aggtctcgcg aaaaagcaag ctttcaagag cgggaatttt gggctcgtca
     2521 cacagcaccg acaccataac tccgggccgg ttttttttca tctggatggg cgttacaaag
     2581 gcatcaaggg cgccagcctc aagcagtctg gtcatcgtgt agccgatgat ctcgcctggc
     2641 aggtcgtcga ggttggtttc cagaacccaa attcggtcgc tcgcggcggg gagattcaca
     2701 tccccgacga acagacgaag gatattggcc tggtccggga cttcgcgcgt tccggctccc
     2761 aggccgattg cttcgatcgt catagccggc aacgacgtga accgctctgc cactgtggtc
     2821 aggatcgcgg ctcccgtggg cgtcgtcatc tcgccgtcga ccatcgatag agcaagggga
     2881 actccgcgca agagttcagc tgtggcgggc gcaggcaggg gcattcgccc atgagccgcc
     2941 ttgacccatc cccggcctgt cgggaccgga ctggcctcat accgatcgac tcccagcaga
     3001 tcaagcccca ccgccgagcc cacgatgtcg acgatcgaat caacggcacc gacttcgtga
     3061 aaatggatct tcgccaggtc gacgccatga actttcgcct cggcttcccc gagcttgagg
     3121 aaaatacgct tggccaggtc ttgctgccga gacgtcagca ggctcttgtc gatgatcgcc
     3181 tcgatatggt gccagtggcg gtggacgtgc tcgtgcggcg cagtgacctt ggcataagtc
     3241 gcccgcatcc caccgcggcg gaccgtctca aacgtcaact cgcaaggcag tccgaggctg
     3301 gccaccgctt cctggatggg acgccgatcg acaccggcat cgaccaaggc cgccaaggtc
     3361 atgtcgcccg cgataccgct gaaacaatcg aaatagccga gtcgcacgcc cttacccctt
     3421 gccctgaatg cggaaacagc ccatgagctc acattctatc cgcccaccaa ccagcggcaa
     3481 ggccggaatg aaacgagccg gagccagtcg cgaagaatgg acgaagatag cgaagtttgc
     3541 agcacgcggg tagccgcaac cttcgctatc ttcgtgcagc gcctattggt cgaggtaact
     3601 ctcatcctcg agccacttga gatatgccag tacctcgtca agcgagtcga cacgttgacc
     3661 cgggacgcgg ccggcgccgt cgagctcacg aagcagcttg aggtcctcga aatactccga
     3721 cgactccagc tcgcgcttga ggcgggcagc cagcggtttt tcgcggcccg agagtaagtc
     3781 catatggtgc tcgattagcc acaacgttcg ctcggtgacg gcgccgcgca gggcctcgac
     3841 gccggcggcg acatgatctt gcggatcaat ggccttgccg acgtcgtgca aaagcgcggc
     3901 taacagaaac tcttcgtcat aagggcgctc gtcgcgagcg agttcgaaga cctgcaagct
     3961 atgaaataac gcatcgcctt cgggatgata tttgggattt tgtttgaccg tctcgagcgg
     4021 ttccaatcgc atcttgtaaa tcgcgaatcg atcgagatga tcggtaattg acagggggac
     4081 tgtatcggct gccggttcag gtgtcgtgtc gagcggatcc ggatccccgc tcgattcacg
     4141 ctggaggaag accaattcct cgcgaacctc ggcatcggac gggagatcac ccgggcggat
     4201 tcggtggccg agtaccgccg ctgccttgcg tttggccagg taaagatcat tggggccagc
     4261 caggtcgagc cagttcggcg gatggtcctt cccagcggga atcagcgtgc tatacagacg
     4321 tcgtgcggcc tcgcgtgcga tttgacggcg gacgcggtcc gatgccaccg gaacccgata
     4381 ccggttgcga tacctgtctt gcatgatcca catgcctcca aagcatttta tccctgccga
     4441 ctccacgagg tcacctttgg aggccaatca ggttgattaa tctgatcaat ttccgcagac
//
LOCUS       DATAQL010001353         5843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4410889, whole genome shotgun sequence.
ACCESSION   DATAQL010001353 DATAQL010000000
VERSION     DATAQL010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4410889"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VHS97_23310"
     CDS             <1..708
                     /locus_tag="VHS97_23310"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3451201.1"
                     /translation="KKNDKLGSESAMKSPAALRDSKTNPVFAVEGNAVPSYNKMPSRS
                     EWTRPKITQLHLGMKTTGWMQAEIPRELHAVPYVPVIDGQINEEIEVYKKYGIVGGAI
                     ERAPGSSGYQPLCLYSFPNYPDGSLRTSVNHLARFLLAYINQGAYGGARILEADTVRF
                     MLTRQNANPPYQGLCWVSLAHDGQPHWFHNGSDPGIRTNMSFRPADGVEAIVFVNRTD
                     VELTKFADRLFEASARF"
     gene            complement(894..1019)
                     /locus_tag="VHS97_23315"
     CDS             complement(894..1019)
                     /locus_tag="VHS97_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451202.1"
                     /translation="MSNRSLAARHRERPVWDAPRDRDDNNPQKPYCVRWDVGPKR"
     gene            complement(1068..1775)
                     /locus_tag="VHS97_23320"
     CDS             complement(1068..1775)
                     /locus_tag="VHS97_23320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451203.1"
                     /translation="MAIAMRASWDGTVLATMTAVVLAAHLAAWGQKTVAKTGPEDQKG
                     LVERWAREVAQYAVETHTEPAAALALKSEPALRWTNPERETDGGLVFLWLGRGRPEAV
                     SCFYRVRFEGRLVEAHEFVSLAVVGLTATLRGRIVWSPPVPGLEPRPVPGAPRPSATA
                     AERLRQLRALAREFHIAVDVDTRPTELRLLPQPVARFGGEDATATALADGTSRSPDGA
                     LFGFVFATDPEAWLVID"
     gene            complement(1884..5180)
                     /locus_tag="VHS97_23325"
     CDS             complement(1884..5180)
                     /locus_tag="VHS97_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451204.1"
                     /translation="MAPETEAAPPPEPWTAERVSEWNAYYDFYVKSAVLLLVFIASCN
                     YAKDSHLWLHLKTGQLIADQGSPVTTDVFSYTANGQSWTDLPWLFQWSQAALYKLVLS
                     LVPVNPTDPTANRESAEQIAIGTLVVLSALIRLATAGILLKIRHRGPGLWWSAIVVAL
                     ALGVVYHPGYGIMMGGIAGPAFVAPATWGLLLFAFLMYILFQAFSLGRAGALWLLIPT
                     FVLWANVDESFFTGLLVFAAAVIGRLLDGTNATAVAGRPEKPAPGAADAPSTQEKTPS
                     PPRVATALIILALSAAACLLNPFTYRAYQAAIYPYLQLFAPAGRVTTVDLLSFFGPWL
                     RQHGGSDWFWLPTFYLVVVALGLGSFLLNANRFSWSRFLPFALLSVIWGIFMQANPMF
                     AVVFAAVVGINGQEWFQERFGTEGRLGGLWRSWSTGGRLVTLGLVFFMIFTGITGRAN
                     SPQDVRFGLGFNVDDFALEAAAFLENHPEIKGNILNTSMPQGNALIWKNAPQRKSYID
                     SRTRLFSRELMEQWEKTRKALSEDDVETWKPLLDQYQISAIMIETGPKGSPLTHRTLS
                     HSPNWIPFYDDGQIVMFGRTDAPASDLAFFKTNRLDPELRAFRTTHPIPGAERPPNPT
                     TWIDDVFKNRTYGRLQARNDAARRWLESGDPTDPAYAPNDPPLPEPARCLMAIQDART
                     ALARSPDDWVAFRTLNEAYRYLMVQEAAMLAGIPIKPESRARIRSVSPNLEQLMSRFQ
                     QRVTVLNFAIQTTPPPRDLAARRELHSLNLELSQLYMTGNALDLARDRLQSVIETSQT
                     EDFPPEVKMQLQGQLDQLNQQMKLLEDKLADLEIERQAGPVEQAQFALSQGGAGKAIA
                     LLAEAERTNVSPAVVKPRLIDLYCNTGQPDKALELLAVGAIDDPNLGSEPGSAALRQG
                     RVYFLLGNYLSTVSLWKDRAIPRVRFDRSSRVLSASSSLTHGEAVQATNLFVALPSTL
                     NQQASWEFDLAMCSLEAGMPEDAATHFTQALTLAPDLGVRPIAAYYLEKLGKPVPPLK
                     RGTAAAVDDKSATKPPVSPIIPAQGPTEPTPPSVTTPNAPEAVTPAAPKDVAKTTNPT
                     PAAANVPTPK"
     gene            complement(5427..>5843)
                     /locus_tag="VHS97_23330"
     CDS             complement(5427..>5843)
                     /locus_tag="VHS97_23330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451205.1"
                     /translation="EPPPPWWRHVLFWSGWLSFPEKAQASFTGFLLAALTACAALLLR
                     WPSGYWLSAALVVIAAVFCLDAGLSYADVAGSRHGVVTRETIARKGIGKDYEPAFDQP
                     LKDGAEFTVLSENGDWIFGHFEGIGDGWLRREHTVR"
BASE COUNT         1158 a         1749 c         1841 g         1095 t
ORIGIN      
        1 aaaaaaaacg acaagctagg ctccgaatcg gcgatgaaat ccccagcggc cctgcgcgat
       61 tcgaagacga atcccgtctt cgccgtggaa gggaatgccg ttccgtccta caacaaaatg
      121 ccctcgcggt cggagtggac ccgtcccaag atcacgcaac tccatttagg gatgaagacg
      181 acgggttgga tgcaggcgga gattccccgc gagttgcacg cggttccgta cgttcctgtg
      241 atcgacggac agatcaacga agagatcgaa gtttacaaga aatatggaat cgtcgggggc
      301 gcgatagagc gtgccccggg tagcagtggc taccagccgc tttgcctgta tagctttccg
      361 aattacccgg atggctctct acgcacgagc gtcaatcatt tggcccgatt tctgctcgct
      421 tacatcaatc agggtgctta cggcggcgcg cgaatcctcg aggctgacac cgtccgcttt
      481 atgctcacgc gccaaaatgc aaatccgcca tatcaagggc tgtgttgggt ttcacttgcg
      541 catgacggcc agccacactg gttccacaac ggcagtgatc cgggcatccg gacgaatatg
      601 tcgttccggc cagcggacgg agtggaagcg atcgtgttcg ttaacaggac cgatgtcgag
      661 ctgacgaaat ttgccgatcg gctttttgag gcatctgccc gattctagag gcgactggcg
      721 gagcaaggga agtatgctat tgctcctcgg agaagtggca aaacgcagcc gctcacccct
      781 ggctgcgcgt cgaagcagat ccgccctgac tgtctcgaag gccatagaag taaaaacgat
      841 tttcttcgaa agaacttcct ccgcgtaatc aatggctttg atcaatgcaa agctcaccgc
      901 ttaggcccga cgtcccagcg gacgcaatag ggcttctgag ggttgttgtc gtcgcggtcg
      961 cgcggggcgt cccagacggg ccgctcgcgg tggcgggcgg cgaggctgcg gttcgacatc
     1021 cgcgcgaacg cgtagttcca ggcggtgccg ccctccgagg cgcggcgcta gtcgatcacg
     1081 agccaggcct ccggatcggt ggcaaacacg aaaccgaaaa gggcgccgtc gggggagcgc
     1141 gaggtaccgt ctgccagtgc ggtcgcggtt gcatcttccc ccccgaaccg ggcgaccggc
     1201 tgcgggagga gccggagttc agtgggcctg gtgtcgacgt cgacggcgat gtgaaactcg
     1261 cgagccaggg cgcggagctg acggagccgc tcggcggcgg ttgccgacgg ccgtggcgcg
     1321 cctgggaccg ggcggggctc cagcccaggt accggcggcg accagacgat ccgcccgcgg
     1381 agcgttgccg tcaggccgac aacggcgagc gacacgaact cgtgcgcttc gacgagccgc
     1441 ccctcgaagc ggacccggta aaaacatgag accgcttccg gccggccccg gccgagccag
     1501 aggaacacta gcccgccgtc agtctcgcgc tccggattgg tccagcgcaa ggccggctcg
     1561 gacttcaagg ccaacgcggc cgctggctct gtgtgcgtct cgactgcata ttgggcgacc
     1621 tcacgcgccc accgctcgac cagccccttc tggtcctcgg gcccggtctt ggcaacggtc
     1681 ttctgtcccc acgcggccag atgtgcagcc agcacgacgg ccgtcatcgt ggccaacacc
     1741 gtgccatccc aacttgcccg catcgcgatc gccatggcat cccccccggc ccaggctaca
     1801 agttgccccg tacggctaca aatataaccg ggcgactcgc acggtcatag gggggtttcg
     1861 gctgcggggt acagatgact aatttactta ggcgtcggca cattcgcggc agcgggagtt
     1921 gggttcgtcg ttttcgcgac atccttcggt gcggctggtg taacggcctc tggcgcattc
     1981 ggtgtggtga cagaaggagg cgtcggctcc gtcggccctt gcgcggggat tatgggacta
     2041 acgggtggct tggttgcgga cttgtcgtcc acagcagccg ctgtgccacg cttgagtggc
     2101 gggacaggtt tgccgagttt ctcgagataa tacgccgcaa tcggccgaac ccccaggtcg
     2161 ggagccaggg tgagggcctg ggtgaaatgc gtggccgcgt cttcgggcat acctgcttcg
     2221 agactgcaca tcgccaggtc gaactcccag ctcgcttgtt ggttcaaagt cgacggcagg
     2281 gcgacgaaca ggttcgtcgc ctggacagcc tccccgtgcg tgagcgaaga cgacgccgat
     2341 aatacccggc tgctgcggtc aaagcggacg cgggggatcg cccggtcttt ccagagtgag
     2401 acggtggaaa gatagttgcc caggaggaaa tacactcgac cctgtcgcag agccgccgag
     2461 ccgggctcgc tgcccaagtt ggggtcgtcg atcgctccga ccgccaagag ctcgagggcc
     2521 ttgtcaggct gaccggtgtt gcagtagagg tcgatgagcc gaggcttgac gactgccggg
     2581 ctgacgttgg tgcgttcggc ttcggcgagc agagcaatgg cctttccggc tcctccctgt
     2641 gacaaggcga attgtgcctg ttcgaccggc ccagcttgtc gctcgatttc gagatcggcc
     2701 agcttgtcct cgagtagttt catttgctgg ttgagctgat cgagctgccc ctggagctgc
     2761 atcttaactt ctggcgggaa gtcttcggtt tgacttgtct cgatcaccga ctgaaggcga
     2821 tcgcgggcca agtccaaggc gtttccagtc atgtaaagct gagaaagctc cagattgagc
     2881 gaatgcagct cgcgccgagc agccaggtcc ctgggaggtg gcgttgtctg gatcgcgaag
     2941 ttgagaaccg tcacgcgttg ttgaaaccgg ctcatcaact gctcaaggtt gggacttact
     3001 gatcggattc gagcccgaga ctcgggcttg atcggaatgc ctgccagcat ggcggcttcc
     3061 tggaccatga ggtaacggta agcttcgttg agcgtgcgaa aggcgaccca gtcgtccgga
     3121 ctgcgcgcaa gcgcggtccg cgcgtcctgg attgccatca agcatcgggc tggctcgggc
     3181 aaaggtggat cattcggcgc gtaggcgggg tcagtcgggt cgcccgactc gagccaccgc
     3241 cgagccgcgt cgtttcgcgc ctgcagtcgg ccataagtgc gattcttgaa cacatcgtca
     3301 atccacgtgg tcgggtttgg cgggcgctcg gcgccgggga tgggatgcgt ggtccgaaac
     3361 gcacgcagct cgggatcgag ccgattggtc ttgaagaacg cgagatcaga agccggtgcg
     3421 tcggtgcggc cgaacatcac gatctggccg tcgtcgtaaa acgggatcca gttcgggctg
     3481 tgcgagagcg ttctgtgagt aagcggagat cccttcggtc ccgtctcgat catgatcgcc
     3541 gagatctggt actggtcgag caacggcttc caggtctcga catcatcttc gctgagcgcc
     3601 ttgcgagttt tttcccactg ttccatgagt tcgcgcgaga acaggcgagt ccggctgtcg
     3661 atgtacgact tgcgctgcgg tgcgtttttc cagatcaagg cgttaccttg aggcatggat
     3721 gtgttcagga tgtttccctt gatctcggga tggttctcca ggaatgccgc ggcctcgagc
     3781 gcgaagtcgt cgacgttgaa tcccagcccg aatcgaacat cctgcggcga gtttgctctc
     3841 cccgtgatgc cggtgaagat catgaagaag acgagtccca gcgtgaccag tcgtccgccg
     3901 gtggaccagc tcctccatag acctcccagc cggccctcgg ttccgaaccg ctcctggaac
     3961 cattcctggc cgttaatccc cacgaccgcg gcaaagacca ccgcgaacat gggattggcc
     4021 tgcatgaata tgccccagat taccgagagc agggcgaacg ggagaaatcg gctccaggaa
     4081 aaccggttcg cattcaacaa aaacgatccg agtcccaggg cgacgacgac cagataaaac
     4141 gtcggcagcc aaaaccagtc gctgccccca tgctggcgga gccagggccc aaagaacgac
     4201 aggaggtcaa cagtcgtgac tctccccgcg ggcgcgaaca gctgcaggta cggatagatc
     4261 gcggcctggt aggcgcggta agtgaacggg ttcaacagac acgcggctgc gctcaatgcc
     4321 aggatgataa gcgcggtcgc cactcgcggc gggcttggcg ttttttcctg tgtactcgga
     4381 gcgtcggctg ctccaggtgc tggcttttcc ggacggcccg caaccgccgt ggcattagtc
     4441 ccgtcgagca aacggccgat gaccgcggcg gcgaacacca ggagtcccgt aaagaaagat
     4501 tcgtcgacgt tcgcccagag cacaaacgtc ggaatcaaca gccagagggc gcccgctctt
     4561 cccagcgaaa acgcctggaa aagtatgtac attagaaagg caaaaagcag taagccccac
     4621 gtcgcgggcg ctacaaaggc aggtccggcg attccaccca tcatgatccc gtagcctgga
     4681 tggtagacga ctcccaaggc cagggccacg acgattgccg accaccataa gcccggcccg
     4741 cgatgacgga tcttgagcaa gatcccggcc gtcgccagcc gtatgagtgc actcaaaacc
     4801 acgagtgttc cgatggcgat ctgttcagcg ctctctcggt tggccgtcgg gtcggtcgga
     4861 ttgacgggga ccaaactcaa caccagcttg taaagcgccg cctgactcca ctgaaacagc
     4921 cacggcaggt cggtccacga ctgtccgttt gccgtatagg agaaaacgtc ggtcgtcacc
     4981 ggcgaccctt ggtcggcgat caactggccg gttttcaaat gcagccaaag gtgcgaatcc
     5041 ttcgcataat tgcacgaagc tatgaatacc agaagaagca ctgctgactt gacgtaaaaa
     5101 tcgtagtaag cgttccattc gctcacacgc tccgccgtcc agggttccgg aggcggcgcg
     5161 gcctccgtct cgggggccaa ggtttcctcc gtggccgtcg cactcgcggt atcgttttct
     5221 ggaacaggtt gtgaaacttc gggttcggtc atcagattca acttataaga tcgagatgaa
     5281 tggccataac gtttggctcg gacgccaccc cgcgcggggg caactttatg ctaggtgccg
     5341 acgaccaagg cggtcaagag gggggcggcc caaagagcag cctgatcgtc ggtttttatt
     5401 cgagataaag gtcgttggtt tgtcattcat cgcaccgtgt gctcacgccg cagccagccg
     5461 tcaccgatgc cttcgaaatg tccgaaaatc cagtcgccgt tctcggagag gaccgtgaat
     5521 tcagcgccgt ccttaagggg ctggtcgaag gccggttcgt agtccttgcc gatccctttg
     5581 cgggcgatgg tttcgcgggt gacgacgcca tgccgcgacc cggcgacgtc ggcgtacgac
     5641 agaccggcat cgaggcagaa gacagcggcg atcacgacga gggccgcgct gagccaatac
     5701 ccgctcggcc agcggagaag cagggccgcg cacgccgtca gagccgccag caaaaatccc
     5761 gtgaacgacg cttgggcctt ctcgggaaac gacaaccagc cgctccagaa caacacgtgt
     5821 ctccaccatg gcggaggcgg ttc
//
LOCUS       DATAQL010001354         6391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3650454, whole genome shotgun sequence.
ACCESSION   DATAQL010001354 DATAQL010000000
VERSION     DATAQL010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3650454"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..873
                     /locus_tag="VHS97_23335"
     CDS             253..873
                     /locus_tag="VHS97_23335"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3451206.1"
                     /translation="MSTAMPAVRRLRIGPRSAGLLLTTAEFDHARFREGWRYELINGV
                     LVVSPTPSRKERHPNDRLGQWLWNYQEQHPLGSSLDLTLPEETIEGKQNRRRADRAIW
                     AGLGRDPEEGEAPTIVVEFVSQGKVAQERDYVAKRAEYREIGVREYWIIDRFRRTLTV
                     FTFSGESAQERVIPENQNYTTPLLPGFELPLGRLLKLADRWAKKRR"
     gene            964..1419
                     /gene="purE"
                     /locus_tag="VHS97_23340"
     CDS             964..1419
                     /gene="purE"
                     /locus_tag="VHS97_23340"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX3451207.1"
                     /translation="MGSKSDWETMRHASELLTELGILHESKIVSAHRTPDRLFRYGRE
                     AEARGLEVLIAGAGGAAHLPGMLAAVTILPVLGVPVESKVLRGVDSLLSIVQMPRGVP
                     VGTLAIGPSGAANAAILAAAILARKYPSIRESLARFRLAQTDAVGESPS"
     gene            1416..2615
                     /locus_tag="VHS97_23345"
     CDS             1416..2615
                     /locus_tag="VHS97_23345"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX3451208.1"
                     /translation="MTTSQRADCTPVARGCGLFPPASLGVIGSGQLGRMFIQAAQRMG
                     YSVGVLSATEETPAAQVAHWTVIGPPDHLPALRSFAQRAEAVTVEFENVSAPALRWLA
                     RRRTVRPGWRTLWICQNRLREKRFLATHSIPHAPWRPVRTAAELDQAVGDLGLPLILK
                     TAASGYDGKGQILVNEASQATGAWASLGRVPCVAEGWIDFAAEVSVVVARGFDAHAVC
                     YPVALNHHERHILDTTLMPAPVGPIVAHEARGLALAVAQALGTVGVLTVEFFLTAAGR
                     LLVNEIAPRPHNSGHLTIEAAVTSQFEQQVRSLCGLPLGLADLLNPAAMVNLLGDLWE
                     EAGGEPHWESALTLDPGVKLHLYGKRTPAAGRKMGHLTVLDSDPEAALHRALAARRAL
                     AAGHRGC"
     gene            2657..3658
                     /locus_tag="VHS97_23350"
     CDS             2657..3658
                     /locus_tag="VHS97_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3451209.1"
                     /translation="MQFAQPGWLWLLILMPLPWLLDRARPRIAWPSLGGFPSRHRIGW
                     FWLRALPAILRGLAIGALAVALARPQTVGGITRIAGQGVAIVVALDHSSSMNAVDFPT
                     DRGTRLISRLEAAKTTFTDFVEGRSDDLVGLVVFANYPELACPPTLDHAFLLETTDAV
                     RSARPLDDGTNIGDAIAVGLETLLATSPKKKVLVLLTDGNNEPGVRHPLPPEEGAVLA
                     RDFGVTMHTIAIGRIGRGTQGIDPGLKIKVTDDAAGPNLALLERLSQLTGGRSFAATD
                     ADALTEVFRTIDALEKSPVRGQILTRYNEHFAPWAGLALCLLILDRLLSLGRLRRLP"
     gene            3737..4693
                     /locus_tag="VHS97_23355"
     CDS             3737..4693
                     /locus_tag="VHS97_23355"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEX3451210.1"
                     /translation="MDQRRLEVQIYQIADDAVLKQNRRDGTGWEWGWADWQRDWMNAT
                     PNRFAYRCLPLTIINQTGWWIKNPVGFTAIWRGPTAPGSVEFRFDASAEIWRSWINSQ
                     FGEGIITWNTPFLFRTKPEGSRLLICGPPNYFKVNAHPLTALIESDWVSMSFTMNWKI
                     MVPNQPVRFERGEPLFQAIPLVSNVCADLETADVSYQKLTDDPDLYRAYLEWDQGRRR
                     FHDQKAAGQVRPDGWQKDYFQGRDATGKEAITHHMTKVKPPEVRGISADPSRAAAGTG
                     SLAGNEVERRDQAVEWLYSRANVAIVMMGQEPDPDAEERPAQ"
     gene            complement(4718..5410)
                     /locus_tag="VHS97_23360"
     CDS             complement(4718..5410)
                     /locus_tag="VHS97_23360"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX3451211.1"
                     /translation="MTTIDREAPTSGLDMVAITAEPLDDRRCRFVVGRPVYPGRWAYF
                     GGEEQARVAPLADRLFAIKGVIGVLIAHDKVTVTLEGQSGIPVLGTAIRTVRALVGGR
                     SGDAASWTSLARRIGSAIREHLAAGEPAVADPPPVHVPNESELRERIQRVLDEQVNPV
                     IAGHGGGVSILDVKDNVVYVQMWGGCQGCGLADMTLKNGVEAAVRDAVPEVGEIIDLT
                     NHRAGKNPFRQK"
     gene            complement(5565..6077)
                     /locus_tag="VHS97_23365"
     CDS             complement(5565..6077)
                     /locus_tag="VHS97_23365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3451212.1"
                     /translation="MSRSKRFSLTLGTADDAAALAALHTAVAENLTRLHGRGAWSTKT
                     SENGVLYAMRTCRVFVAREDAEIVATMRLATKKPWAIDVSYFTPCSRPLYLLAMAVAP
                     ARQRKGLGRKCLEEATRAARRWPADAVRLDAFDAAAGAGGFYARCGWSEVGRASYRNT
                     PLIYYEFLLK"
BASE COUNT         1177 a         2059 c         1940 g         1215 t
ORIGIN      
        1 ccccgcagcc aattacgccc agtcgagtct ctcctgaatt catcgttact ccatttcgag
       61 ttcggacttc gtccgtgcaa ggatcgcttt tctcgtcatg atcgattccg agattgtcgt
      121 ccctggtccc gagtcggtca attcacgggc gccgaagacg gatcgtccca atctctgaat
      181 tgaggtagaa tgaaagaatt ggtgattgag gcacgaagta acccgttctg gctttggacg
      241 cgagttcacg ccatgtcgac agcaatgccc gccgtccgcc gcctccgaat cggacctcga
      301 tcggctggac tgcttctcac aaccgccgag ttcgatcacg cccgcttccg cgaaggttgg
      361 cgttatgaat tgatcaacgg agtcctcgtt gtgtccccaa cgccatcacg caaggagcgc
      421 catcccaatg accgattagg acaatggctc tggaattatc aagaacagca tcccctaggc
      481 tcctcactgg acttgacatt gcccgaggag accatcgagg gcaaacaaaa ccgtaggcgg
      541 gcggaccgtg cgatctgggc cggcctcggg cgcgatccag aagagggcga ggccccgaca
      601 atcgtggtcg aatttgtctc tcagggtaag gttgcccaag agcgcgatta cgtcgccaag
      661 cgggccgagt atcgtgagat cggcgttcga gaatactgga tcatcgaccg cttccgccgg
      721 accttgaccg ttttcacctt cagcggcgag agcgcccagg agcgcgtcat cccggagaac
      781 cagaactaca cgaccccgtt acttcccgga ttcgagcttc cactgggccg tttgttgaaa
      841 ctggccgacc gctgggcaaa gaagcggcgt tgaatcgttg ccctcacccc cccctgcctc
      901 gtttttctct tgagtgatat ggcgaccgag aattcagaag ccggaccgct tgtcggcgta
      961 gtcatgggca gcaaatccga ctgggagacg atgcgtcatg cctccgagct cttgaccgag
     1021 ttgggcatac ttcatgagtc gaaaatcgtc tcggcccatc ggacgccaga tcgcttgttc
     1081 cgctatggcc gcgaggccga ggcacgaggc ctggaagtct tgatcgcggg cgccggaggg
     1141 gcggcgcact tgccgggtat gctggcagcg gtcacgatcc tgccggtact gggcgtgcct
     1201 gtggagagca aagtgctgcg cggggtcgac tcgctgttgt cgatcgtgca gatgccgcgc
     1261 ggcgtcccgg tcggcacgct cgccattggt ccctcgggcg cggccaacgc ggcgatactg
     1321 gccgcggcga tactggcgcg gaagtacccg tcgatccgtg agtctctcgc tcgttttcgc
     1381 ttggctcaga ccgacgcggt aggagaatcg ccctcatgac cacgtcccag cgcgccgact
     1441 gtaccccggt ggcacgtggc tgcgggcttt tcccaccagc atcgctgggg gtgatcggca
     1501 gtggccagct cggccggatg ttcatccagg cggctcagcg aatgggatac agcgtcggag
     1561 tattgagcgc gaccgaagag acgcccgccg ctcaagtcgc acactggacg gtcatcggtc
     1621 ctcctgatca tctgccggcg cttcggtcct ttgcccagcg cgccgaggct gtgaccgttg
     1681 agtttgaaaa tgtgtcggca cctgcgctac ggtggctggc gcgccggcgc actgtgcgtc
     1741 ccggttggcg taccttgtgg atctgccaga atcggctgcg cgaaaagcgc ttcttggcca
     1801 cgcactcgat ccctcacgct ccctggcggc cggtccgcac cgccgccgaa cttgaccagg
     1861 cggtcggcga cctcgggctg ccgctgattc tcaaaaccgc ggcatcgggc tatgacggca
     1921 agggtcagat actcgtaaac gaagcgtcgc aggccactgg cgcgtgggcc agtctcggcc
     1981 gagtcccctg tgtcgcggag gggtggatcg acttcgccgc ggaagtctcg gtcgtcgtgg
     2041 cccgcggctt cgatgctcac gccgtctgct atccggtcgc gcttaaccac cacgagcgac
     2101 atattctcga caccacactc atgcccgcgc cggtaggccc gatcgtcgcg cacgaggctc
     2161 gcggtttggc cctggctgtc gcgcaggcgc tcggcaccgt cggcgtcctg accgtcgaat
     2221 tcttcctgac cgcggcaggc aggctcttgg tcaacgaaat cgctccccgc ccccacaact
     2281 cgggacacct cacgatcgaa gcggccgtca ccagccagtt cgaacagcag gtccggtcat
     2341 tgtgcgggtt gcctctgggc ctggccgacc tgctgaatcc ggcggcgatg gtcaatctgc
     2401 ttggcgactt atgggaagag gcaggcggcg agccgcactg ggaatccgct ttgacgctcg
     2461 atcctggcgt caaacttcac ctttacggca agcggacgcc agccgcgggc cgaaagatgg
     2521 gccatctgac tgtcctcgat tccgatccgg aggctgcgct ccaccgcgcc ttggcagcgc
     2581 gtcgcgcgct cgccgccggc catcgcggct gctgatgctc tgtcaacact cccgatatcg
     2641 ctcgacggtg cagcgcatgc aatttgctca acctggttgg ctctggctct tgatcttgat
     2701 gccgcttccg tggttgctcg accgcgcccg gccccggatc gcctggccaa gcctgggtgg
     2761 atttccctcg cgtcatcgca tcggttggtt ctggttacgg gccctaccgg ccatcctgcg
     2821 tgggctggcg atcggtgctt tggcggttgc attggcccgg ccccagaccg tcggcggcat
     2881 aacccgcatc gctggacaag gggtcgcgat cgtcgtcgcg ctcgatcata gctccagcat
     2941 gaacgccgtc gattttccca ccgaccgcgg cacccgcttg atatcgcgcc tcgaagcggc
     3001 caagacgacg tttaccgact ttgtcgaagg acgttccgac gacctggttg gcctggtcgt
     3061 tttcgccaat taccccgagc tggcatgtcc cccgaccttg gaccatgcct tcttgctgga
     3121 aacaaccgac gccgtgcgct cagcgcggcc gctcgacgac ggcacaaaca tcggtgatgc
     3181 catcgcggta ggactcgaaa cactcctggc cacctcccca aagaagaagg ttctcgtgct
     3241 cttgaccgac ggcaacaatg agccgggcgt ccgccatcca cttccccccg aggagggggc
     3301 tgtcctggcc cgcgatttcg gtgtgaccat gcacacgatc gcgatcggcc ggatcggtag
     3361 gggcacgcaa ggcattgatc ctggattgaa gataaaggtc acagatgatg ccgcgggtcc
     3421 gaatctagcg cttctggagc gcctgtccca gctcacgggc ggacgctcgt tcgcggcgac
     3481 tgacgccgat gcgctgaccg aggtattccg gacaatcgac gctctcgaaa aaagtcccgt
     3541 gcggggacag atcctcactc gctacaacga gcacttcgcc ccttgggccg ggctggctct
     3601 gtgcttgctc atcctcgaca ggcttctttc gctcggacga ttgcggcggt tgccctaacc
     3661 gagacgatcc ttcaaaataa ggtgcgtgcg cttgctggtc cggccgacca gactcgggaa
     3721 tcaaggagaa tatctcatgg atcagcgtcg actcgaggtc cagatctacc agatcgccga
     3781 cgatgccgtg ctgaaacaaa accgccgcga tggaacgggc tgggaatggg gctgggccga
     3841 ctggcagcgc gactggatga atgcgacgcc gaaccggttt gcgtatcgct gtcttccact
     3901 cacgatcatc aatcagaccg gttggtggat caaaaacccc gttggattca cagcaatctg
     3961 gcgcgggccg acagcaccgg gaagcgtgga attccgtttc gacgcctcgg ccgaaatctg
     4021 gcggagctgg atcaacagcc aattcggtga aggcatcatc acttggaaca caccgtttct
     4081 ctttcgcacc aagccggagg gctcgcggct cttgatctgc gggccgccca actacttcaa
     4141 ggtcaacgca catccgctga ccgcgttgat cgaaagtgac tgggtcagca tgtcgttcac
     4201 catgaactgg aagatcatgg tgccaaacca gccggtcagg ttcgagcgcg gcgaaccctt
     4261 gttccaggcg attccgctcg tgagcaacgt ctgtgccgac ctcgaaacgg ccgacgtgtc
     4321 ctatcaaaaa ctgaccgacg accccgactt gtaccgcgct tatcttgaat gggaccaagg
     4381 gcgcaggcga ttccacgacc agaaagccgc tggacaggtc cggcccgatg gttggcagaa
     4441 agactatttt cagggccgcg acgccactgg gaaggaagcc atcactcatc atatgacgaa
     4501 agtgaaaccg ccagaagtac gaggcatctc ggccgacccg agccgtgcgg ccgccgggac
     4561 gggtagcctg gcgggtaacg aggtggaacg gcgggatcaa gccgtggaat ggctatactc
     4621 gcgggccaac gtggcgatcg tgatgatggg gcaagagcca gatcccgacg ccgaagagcg
     4681 tcctgctcaa tagccgcttg agtcgaccgc ttcgccgtta tttctggcga aacggattct
     4741 ttccagcccg gtgattggtg aggtcgatga tctcgccgac ctcggggacg gcgtcgcgca
     4801 cggcggcctc gacgccattc ttgagggtca tgtcggccag tccgcagccc tggcagcctc
     4861 cccacatctg cacgtacaca acgttgtctt tgacgtcgag gatcgaaacc ccaccgccgt
     4921 ggccggcgat caccgggttg acttgctcgt ccagcacgcg ctggattcgc tcgcgcagtt
     4981 cggactcgtt cggtacgtgt accgggggcg gatcggcgac ggccggctcg cccgcggcca
     5041 ggtgctcacg aatcgcggaa ccgatccgcc gcgccagcga ggtccagctg gccgcgtcgc
     5101 ccgatcggcc gccaacaagc gcgcggacgg tgcgtatcgc cgtaccgagg acaggaatcc
     5161 ccgattgtcc ttcgagggtt accgtaacct tgtcatgggc gatcaataca ccgatcaccc
     5221 ccttgatcgc gaagagccga tcggccaatg gcgcgactcg tgcctgttct tccccaccga
     5281 agtaggccca acgcccaggg tagaccggcc gcccgaccac gaaccgacag cgacggtcat
     5341 cgagcggctc ggccgtgatg gccaccatat ccaggccgct ggtcggggct tcgcgatcga
     5401 tcgtggtcaa acgccaggcc ctccgccatc gcgttcggtc attttctgaa catcactaga
     5461 tacacacgat ggagacgcat agaattcttc cttcattcta gttaactacc ccgcgcgcag
     5521 caacttcaga cggtggggat ccgcgatccc gtcgaaccat gcggctactt gagtaggaac
     5581 tcgtagtaga ttagcggtgt gtttcgatag gaagctcgtc ccacttcgga ccaaccgcac
     5641 cgcgcgtaga agccgccggc cccggccgcg gcgtcaaacg catcgaggcg aaccgcatcc
     5701 gccggccacc gccgcgcagc tcgcgtggct tcttccaggc actttctgcc aagtccttta
     5761 cgttgccttg ccggggccac cgccatcgcc aggagataca gtggccggga gcaaggcgta
     5821 aaatagctta catcgatggc ccaaggcttc ttggtcgcca gtcgcattgt ggcaacaatc
     5881 tcagcgtctt cgcgtgccac gaagacgcga cacgtacgca ttgcatagag gacgccgttc
     5941 tcggacgtct tggtcgacca ggctccgcgg ccgtgaagac gggtcagatt ttcggcgacc
     6001 gcagtgtgca gcgcggccag ggccgccgca tcatcggctg tgccgagcgt cagactgaaa
     6061 cgctttgaac gcgacataac cggccttaac aacgacgacc caatcacccg tccagctcgt
     6121 cgcgcgtgcg atgcagccgc ctggccagga gtccgcccgg cagggcgacg atcggggtca
     6181 aggccgttac tcgcgaagcc ctcagggacg tgctacaatc ctcacccatg aatcgtcgaa
     6241 ccttccttct aggtggtctg gtggccactg gggctgctcc cgtgatcgcc gtcgctcagc
     6301 cgcaagacaa gctggacgtc gcagcggatg tcttgaccac cgctgtggcg ggaggacagg
     6361 tggccgccgc cgtcctctac gtccgccaca g
//
LOCUS       DATAQL010001355         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5023466, whole genome shotgun sequence.
ACCESSION   DATAQL010001355 DATAQL010000000
VERSION     DATAQL010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5023466"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1906
                     /gene="guaA"
                     /locus_tag="VHS97_23370"
     CDS             344..1906
                     /gene="guaA"
                     /locus_tag="VHS97_23370"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX3451213.1"
                     /translation="MAANGPASRPVLVLDFGAQYVQLIARRVRERHAFARIVRHDITA
                     ERVRQLDPLALILSGGPSSVYEPGAPGCDPAIFQLGLPILGICYGMQLACKALGAKVD
                     ASPAREFGRASCHVLDPAEPLFRDVPHETIVWMSHGDQIHDAGSDFRPLATTATCPVA
                     AARHCDLPIYGLQFHPEVSHTPYGSLILGNFLDRICRNPRTWTMEAFIERSLHEMTSR
                     VGPNERVVCGLSGGVDSAVCAALLAKALGTRVVCVFVDTGLLRLGERESVALAFGSRT
                     SAELRVIDASQRFLKTLKGVVDPQEKRRRIGHEFIEVFRDEARSIPGARYLAQGTLYP
                     DVIESGGEPDGPAATIKIHHNVGGLPEELGFELIEPLRDLFKDEVRRLGIELGLPDSL
                     VWRHPFPGPGLAVRCLGEVTAERLEILSQADAIFLDELHQAGLERRVAQAFAVLLPVQ
                     AVGVMGDARTYESVLALRAVETDDFMTADWSRLSHDLLASASTRITNTVKGVNRVVYD
                     ITSKPPGTIEWE"
BASE COUNT          346 a          604 c          603 g          396 t
ORIGIN      
        1 aatatttcaa accgacgccg atttcacggt gtaaccgatt gagacgccat gactggggcc
       61 ggcgaatggc gttggaggtt gggttaatca atgggccaat cgcgccatcg cgcttggtac
      121 agcggatgat atgtgtcgac ggccagcgga gcaagcaccc tgatgggatt gctctgaaat
      181 accgtgaatt ttatggcgac gagttggtcg ctctagcgaa tgcccacgcg ggacgctgta
      241 tcgaattggc gtgccgagca ccgtctggta gacttggtgc acgtatccat aggtatctct
      301 ctcaaacgtt tcggcgattc cggttctttc gagaaaaaag ctcatggcgg ctaatggccc
      361 cgcgtcccgg cccgtcctcg ttcttgattt cggtgcccag tacgtccagc tcatcgcccg
      421 cagggttcgc gagcggcacg cctttgcccg catcgtccgc cacgatatta ccgccgagcg
      481 ggttcgccag ctcgatccgc ttgcgctgat cctctcgggc gggccgtcga gcgtgtacga
      541 gcccggagcg ccgggttgcg atccggcgat cttccagctc ggattgccga tcttgggaat
      601 ctgttacggg atgcaattgg cctgcaaagc actcggcgcc aaggtcgatg cgtcccccgc
      661 gcgcgaattc ggccgcgctt catgccacgt cctcgaccct gccgagcccc tctttcgcga
      721 tgtacctcac gaaacgatcg tgtggatgag ccatggcgac cagattcacg acgccggcag
      781 cgatttccgg ccgctcgcga ccactgcgac ctgcccggtc gcggcggccc gccattgtga
      841 tctgccgatt tacggactcc agttccatcc cgaggtttcg catacgccgt atggctcact
      901 catcctgggg aacttcctgg accggatctg ccgcaaccca cggacctgga ccatggaggc
      961 gtttatcgaa cggtcgctgc atgagatgac cagccgcgta gggccgaacg agcgcgtggt
     1021 ttgcggctta tcaggcgggg tcgactcggc cgtttgcgcg gccttgcttg ccaaggctct
     1081 gggaactcgg gtcgtctgcg tcttcgtgga cacagggctg ctccgcctgg gagagcgcga
     1141 atcagtcgcc cttgcgttcg gctcacgcac gagtgccgag ctgcgtgtga tcgatgcgtc
     1201 gcaacgattt ctcaagaccc taaagggcgt cgtcgacccg caagaaaagc ggcgccgaat
     1261 tggccatgag tttatcgagg ttttccgcga cgaggcccgc tcgatccccg gagcgcgcta
     1321 tctggcgcaa ggaacactgt accccgatgt gattgagagc ggcggcgagc ccgacggacc
     1381 cgcggcgacg attaagatcc atcacaacgt gggcggactc cccgaagaac tgggcttcga
     1441 gctcatcgag ccgttgcgcg accttttcaa agatgaagtc cgccggctgg ggatcgagct
     1501 gggtctgccg gattccttgg tatggcgcca tccttttccc ggtccgggac tggcggtacg
     1561 ctgcctggga gaggtaaccg ccgagcggct cgagattctc agccaggccg atgccatttt
     1621 cctcgatgag ctgcaccagg ccggtctcga gcgcagagtc gcccaggctt tcgccgttct
     1681 gttgccggtc caggccgtgg gtgtgatggg ggatgcgcgc acctatgaaa gcgtgctcgc
     1741 tctccgtgcg gtcgagaccg acgacttcat gacggccgac tggtcccggc tttcacatga
     1801 tttacttgcc agcgcctcca cgcgaatcac caacaccgtc aaaggcgtca accgcgtcgt
     1861 atacgacatt accagtaagc cccccggcac gatcgagtgg gagtgatggc aggcgctcgg
     1921 cgaatcggca taatccgcca ggcgaaacg
//
LOCUS       DATAQL010001356         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4411275, whole genome shotgun sequence.
ACCESSION   DATAQL010001356 DATAQL010000000
VERSION     DATAQL010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4411275"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VHS97_23375"
     CDS             <1..1629
                     /locus_tag="VHS97_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX3451214.1"
                     /translation="GSGKSTLMHALITNLALNYSPDEIDLYLIDFKKGVEFKVYATRE
                     LPHASVVAIESEREFGISVLERLDAELRLRADRFRDAGVQDVNGYRNVPGTPPLPRIL
                     LIVDEFQEFFVEEDKLAQEAALWLDRLVRQGRAFGVHIILGSQSLGGAFTLARSTLGQ
                     MAVRIALQCSENDAHLILSENNIAPRMLSRPGEAIYNDANGAPEGNHFFQVVWLSDEQ
                     REAYLTQLHEMARQKKPVLARTPIVFEGDAPADLARNALLNVRIAAPAWPDSPRSAPA
                     WVGDAISIKDPTAALFRRQGGNHLLIVGQNDEAAIGVMISTLLSLGAQYPPAKSTTVR
                     SGARVYIIDGTPEDHPSTGVLARIAAVLPHGVDVGDWREVPRILAEVDAELKRRQEAK
                     TDGPELFLFIHDLSRFRDLRRREDEFSFSKRDEDASPADHLDAILREGPLLGVHVIAW
                     ADTVNNLNRCFTHQTLREFEMRVLFQMSPTDSGHLLDAPHASKLGPHRAFFSSEEQNR
                     IEKFRPYGVPGDEWLRWMRDQLANRAAALGVGGS"
     gene            complement(1719..>3203)
                     /locus_tag="VHS97_23380"
     CDS             complement(1719..>3203)
                     /locus_tag="VHS97_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451215.1"
                     /translation="SHRFTVVPVGGLEAIEPSRSSTRFAKPLTEISLRTDFPDPPGDR
                     DIDLVVLGESSAEGVPYQRWASVGKIISWKLQQVIPDRPIRLQVLARSGDTLEKQHQI
                     LSNLNRRPDMLVVYCGHNEFYSRLWWSRNLDHYVVDQRPYRFRVIIDKLERRSSVCTL
                     IREIADKCRIAIPPPAGTRRDLVDVPVYTAEEYRLLLADFQNRLDEMVSYAERVGALP
                     ILILPPANDAGFEPNRSYLPPTTPRAERNAFERRFLAARRLETADPAAARQAYQGLLA
                     RQPCFAEAHYRLAELLEQSGDWDGAYQQYVAARDTDGMPMRCPSSFQQAYRDVADRHD
                     CILIDGQSYFHAIGRHGLLDDELFQDAMHPSLRGQIALAQAVLVALQTRHALGWPANS
                     PLPVIDPSQCASHFGIDKSAWRSIAMWWNGFNVLVTPLRYDRNMRWRKRAAAIAAVEL
                     IDAGVAPEKAGLPNVGIPAGIPVIDNLPVPSSGFAMPITPCLTE"
BASE COUNT          628 a          959 c         1004 g          612 t
ORIGIN      
        1 ggttcgggca agtcgacctt gatgcacgcc ctgatcacca atctcgcgct caattacagc
       61 cccgacgaga tcgaccttta tctgatcgac ttcaagaagg gggtcgaatt caaggtttac
      121 gcgacgcgcg agctgccaca cgcgagcgtc gtcgcgatcg agagcgagcg cgagtttggc
      181 atcagcgtgc tcgagcggct cgacgccgag ctgcgcctcc gcgccgaccg tttccgcgat
      241 gcaggcgtgc aagacgtaaa cggctatcgc aatgtaccgg gaactccccc actgccccgt
      301 atactgctca tcgtcgacga gtttcaggag ttctttgtcg aagaagacaa gctggcgcag
      361 gaggcggcgt tatggctgga ccgcctggta cggcagggtc gtgcgtttgg tgttcacatc
      421 atcttaggat cgcagagtct ggggggcgct ttcacgttgg cccgcagcac gctgggccag
      481 atggccgtac ggatcgcgct ccagtgcagc gagaacgacg cccacttgat cctgagcgag
      541 aataatatcg cgccgcgaat gctgtcgcgt ccgggcgaag cgatttacaa cgatgccaac
      601 ggtgcgcccg aggggaacca cttcttccag gtggtctggc tttcggatga gcagcgtgag
      661 gcctatctga cgcagctcca tgagatggcc cgccagaaga agccggtctt ggcgcgcacc
      721 ccgatcgttt tcgaggggga tgcgccggcc gacctggcgc gaaacgcgct cttgaacgtg
      781 cggatcgccg ccccggcgtg gcccgattca cctcgttcgg cgccggcgtg ggtgggcgac
      841 gcgatctcga tcaaggatcc gaccgcggcc ctttttcggc gccaaggggg taatcatctc
      901 ttgatcgtgg gccagaacga tgaggccgcg atcggagtca tgatctcgac gctcctctcc
      961 ctgggcgctc agtatccgcc ggccaaatcg acgacggttc gctcgggggc acgcgtctac
     1021 attattgatg gcacgcccga agaccacccc agcaccggag tcctcgcgcg catcgccgcg
     1081 gtcttgccgc acggcgttga cgtcggcgat tggcgcgagg ttcctcgaat cctcgccgaa
     1141 gtcgacgccg agctcaagcg ccgccaggag gccaagaccg acggtcctga gttgttcttg
     1201 ttcatccacg acctgtcgcg gttccgcgat ctccgccgcc gcgaagacga gttcagcttt
     1261 tcgaaacgag acgaagacgc cagccccgcc gaccaccttg atgcaattct ccgtgagggg
     1321 ccgctcctgg gcgtgcacgt catcgcctgg gcggatacgg tgaataacct gaatcgctgc
     1381 ttcacgcatc agactctgcg cgaattcgag atgcgggttt tgttccagat gagcccgacc
     1441 gactcgggcc atttgctcga cgctccccac gcgagcaagc ttgggccgca tcgcgcattc
     1501 ttttcgagtg aagagcaaaa ccggatcgag aagttccgcc catacggcgt tcctggcgac
     1561 gaatggctcc gctggatgcg cgatcagctc gcgaaccgcg ccgcggcgct cggagtgggc
     1621 ggatcgtgac gcggcgccgg tttctttgaa tggagagtcc gggaatgagt gaatcccagc
     1681 agggcgcgcc gctccattcc agtctgcgat gcgcgcgatc attcggtcag gcatggcgtg
     1741 atcggcattg cgaatcctga actgggtaca ggtaggttgt caatgaccgg tattcccgcc
     1801 ggaattccta cgttgggtaa tccggccttt tcgggagcaa cgcccgcatc gatcagctcg
     1861 acggcggcga tggccgccgc tcgcttgcgc cagcgcatgt tccgatcgta ccggagcggg
     1921 gtaaccagaa cgttgaatcc gttccaccac attgcgattg atcgccaggc gctcttgtcg
     1981 attccaaaat ggctcgcaca ttgagacggg tcgatcactg gaagagggga atttgccggc
     2041 cagcccaacg cgtgtctcgt ttgcaaagcc accaagaccg cttgcgccaa cgcgatctga
     2101 ccgcgcagcg aggggtgcat cgcgtcttgg aaaagctcat cgtccaaaag cccgtggcga
     2161 ccgatggcat gaaagtatga ctgcccgtca atcaagatac agtcgtgacg atcggcgacg
     2221 tcgcggtaag cttgttggaa ggagctcggg catctcatcg gcatgccgtc ggtatccctc
     2281 gccgcgacgt attgctgata ggccccatcc cagtcgccgg actgttcgag gagttcggct
     2341 aaacggtaat gggcttccgc gaagcacggt tggcgtgcga ggagtccttg gtaggcctgt
     2401 cgggcagcgg ccggatcggc agtttcaagc cggcgagccg ccaaaaaccg gcgctcgaat
     2461 gcgttgcgct cggcccgcgg agtcgtcggt ggcaggtacg accgattggg ttcaaaacct
     2521 gcatcgttgg ctggcggcag gatgaggatt ggcaaggctc cgacccgctc ggcataagag
     2581 accatctcgt ccaaccggtt ctggaagtcg gccaggagca aacgatattc ctccgcagta
     2641 tagacgggta catcgaccaa gtcccgcctg gtgccggcag gaggcggaat cgcgatccgg
     2701 catttatcgg cgatttcacg gatcagcgta cagaccgaag agcgtcgctc caacttgtcg
     2761 atgatgacgc gaaagcggta cgggcgttga tcgacaacgt agtgatcgag gttgcgcgac
     2821 caccagagac gcgaatagaa ctcgttgtgc ccgcaataaa ccaccaacat atcggggcgg
     2881 cggttcaagt tcgacaggat ctggtgctgc ttttccaagg tgtctcccga ccttgccagc
     2941 acctgaaggc ggatgggtcg atccggaatt acttgttgaa gcttccagga gatgatcttg
     3001 ccgaccgaag cccaccgctg atacggaact ccctcggcgc ttgactcgcc aaggactacg
     3061 agatcgatat cgcggtcgcc cggtggatcg gggaaatccg tccggaggct gatttccgta
     3121 agtggtttcg caaagcgcgt cgaagaacgc gaaggctcaa tggcctctag accaccgaca
     3181 ggaacgaccg tgaaccgatg aga
//
LOCUS       DATAQL010001357         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3956131, whole genome shotgun sequence.
ACCESSION   DATAQL010001357 DATAQL010000000
VERSION     DATAQL010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3956131"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VHS97_23385"
     CDS             <1..798
                     /locus_tag="VHS97_23385"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3451216.1"
                     /translation="AYQTYFSVPEAAGQTDVTSGLWYAFTVGSVHVISIANDDVVYQD
                     GGNSYVRGYSAGAQKAWLEKELAAARNDRAIDWIVVCMHQVAISTVDKFNGADLGIRQ
                     EWVPLFDKYGVDLVVCGHEHHYERSHPIRGQEANPTLTPVTAATATDVIDTTKGTVHM
                     VIGGGGTSVPSNTLFFNPPQCRVITAVGAPDPVTGKRPPVYVKEPAQWSAVRNAAHSY
                     GLAAFAVDPGPSRGGLTTIKVTYYDVVGADGQLAPFESFTLRRPCRD"
     gene            1077..1754
                     /locus_tag="VHS97_23390"
     CDS             1077..1754
                     /locus_tag="VHS97_23390"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3451217.1"
                     /translation="MTSSPIVFLVDVDNTLIDNDHIQADLKRHLEREFGAACRDRYWA
                     ILEQLFTDLGYRDYLGALQRYRVEHPQDINLLSMSSYLVDYPFANRLYPGALDVLERM
                     RGWGQTVILSDGDVVFQPRKVERSGIFEAVDGHVLIYIHKEEELVDVEQRYPASRYVL
                     VDDKPRILAAVKKIWGDRVMTILPRQGQFALGDDAPSYPPADLTVERIGDLVAYDIPA
                     LFTVKVT"
     gene            1754..>2263
                     /locus_tag="VHS97_23395"
     CDS             1754..>2263
                     /locus_tag="VHS97_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX3451218.1"
                     /translation="MKATQRLHDLGQSIWLDNITRDLMTSGTLKNYIDGLSVTGLTSN
                     PTIFDHAIKSSTSYDASIREKLKEGKSGEKLFFELALDDLTRAAGLFLPIHQQTNGVD
                     GWVSLEVSPLLAHDTASTLAAAKDLHARAGRPNLLIKIPGTKEGLPAIEEAIFAGIPI
                     NVTLLFSREH"
BASE COUNT          441 a          700 c          678 g          444 t
ORIGIN      
        1 gcctatcaga cctatttctc ggtgcccgag gcggccggcc agacggatgt gaccagcgga
       61 ctttggtatg cgttcaccgt cggctcggtg catgtgatca gcatcgccaa tgacgacgtc
      121 gtgtaccagg acggcggcaa ctcctacgtc cgaggctatt cggccggcgc tcagaaggcc
      181 tggctggaaa aggaattggc cgctgcccgc aatgatcgtg cgatcgactg gatcgtggtg
      241 tgcatgcacc aggtcgccat ctccaccgtc gacaagttca acggtgcgga cctcgggatc
      301 cgccaggaat gggtgccgct gttcgacaaa tacggcgtcg acctggttgt ttgcggacac
      361 gagcaccact acgaacgctc acacccgatc cgcggccagg aagcaaatcc caccttgacc
      421 ccggttactg ctgcaaccgc caccgacgtg atcgacacca ccaagggaac ggtgcacatg
      481 gtcatcggcg gcggcgggac ctcggtcccg tcgaacacgc tgttcttcaa cccgccgcaa
      541 tgccgcgtga ttactgcggt gggggcgcct gacccggtta ccggcaagcg ccctccggtc
      601 tatgtgaagg agccggcgca atggtccgcg gtgaggaacg ctgcgcattc ctacggcttg
      661 gcggcattcg ccgtcgaccc gggtccaagc cgcggcggcc tgacgacgat caaggtgact
      721 tattacgacg tggtcggggc agacggtcag ctggccccgt tcgagagctt cacgctgcgc
      781 cgcccctgcc gcgattagcc tcggcgacgg gcgccgaagt ttcagaagct gcaacgcctt
      841 ttcatttccg cttcatttcc gcagtcggcg agcgccggtc cgatcacaaa actgaaactt
      901 aacccatcat aaaatcgcga agcgtatttc cccaatccga ccgttgccgg accgcgaaca
      961 gcttaaggcg gctttaggct cgactcccgc gcggttggct ggttgcgcca cttgacgagc
     1021 acctcggacc gggcgtcttt ggagaagcag gctttgccgg aaaggaaggt agcgttgtga
     1081 cctcgagccc gatcgtcttc cttgtcgatg tcgacaacac gctgatcgac aacgaccata
     1141 tccaagccga tctcaagcgg catctcgaac gcgaattcgg tgcggcctgc cgcgaccgct
     1201 attgggcgat cttggagcag ctgtttaccg atctaggtta ccgggattac ctcggagcgt
     1261 tgcaacgcta tcgggtcgag cacccgcagg acataaatct tctgtcgatg tcctcttatc
     1321 tggtggatta cccgttcgcc aaccgcctct atcccggggc gctcgatgtc ctcgagcgga
     1381 tgcgcgggtg ggggcagacg gtgatcctgt cggacggcga cgttgtcttt cagccgcgca
     1441 aggtcgagcg ctccggaatc ttcgaggcgg tcgacggcca tgtgctgatc tatatccata
     1501 aggaggagga actcgtcgac gttgagcaac gttaccctgc gagccgatat gtcctggtcg
     1561 atgacaagcc gcggattctg gctgcggtca aaaagatttg gggcgatcgc gtgatgacga
     1621 tactgccccg tcaaggtcag ttcgctctcg gcgacgacgc gccctcgtat cctcccgccg
     1681 acctgactgt cgaacgcata ggcgacctcg tcgcttacga tattcccgcc ctcttcacag
     1741 taaaggtgac atgatgaagg cgacccaacg gctccatgac ctgggccaga gcatctggct
     1801 cgacaacatc acccgcgacc tgatgacgtc aggcacgctt aaaaactaca tcgacgggct
     1861 gtcggtaacc gggttgacct cgaacccgac gatcttcgat cacgcgatca agagcagcac
     1921 ttcctacgac gcgtcgatcc gcgagaagct caaggaaggc aagtcggggg agaagctgtt
     1981 ttttgagctg gcgctggacg accttacccg ggccgccggc ctgtttcttc cgatccatca
     2041 gcagaccaac ggggtggatg ggtgggtctc gctcgaggtg tcgccgctcc tcgcccatga
     2101 caccgcgagc acccttgccg cagcgaaaga tctgcatgcg cgagcggggc ggccaaatct
     2161 gctgattaag attccgggca ccaaggaagg ccttcccgcg atcgaggagg cgattttcgc
     2221 cggcattccg ataaacgtga ccttgttgtt ttcgcgcgag cat
//
LOCUS       DATAQL010001358         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3191560, whole genome shotgun sequence.
ACCESSION   DATAQL010001358 DATAQL010000000
VERSION     DATAQL010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3191560"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..984
                     /locus_tag="VHS97_23400"
     CDS             127..984
                     /locus_tag="VHS97_23400"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX3451219.1"
                     /translation="MQLTPAAFDDIRKAVHDLCGIVIAPDKQYLVKARLEPILLTNGL
                     ASYELLVRQLREPNSVLLRDQTVEAITTNETSFNRDGHPFDALRSFIIPELASRLIER
                     RATTRLPHSQARIWSAAVSTGQEAYSVAMAVADCLVARSAPGVTLDDFPILASDISSK
                     ALAFAREARYSSAEVDRGLSALERARFVRQENGAWIVDAALRRMVEFRRLNLVQPLPE
                     LGTFDLILCRNLLIYLDDATRRRLCRGLHSALNPRGILMIGAAESLYGVTDAFATERF
                     GSTVVYRKP"
     gene            complement(1016..2125)
                     /locus_tag="VHS97_23405"
     CDS             complement(1016..2125)
                     /locus_tag="VHS97_23405"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3451220.1"
                     /translation="MWQRVWTLVVKELLAALRDPRGRYILIVPPILQMLVFSVAATQE
                     VKNVRMAVLNEDYGTAARDLIARFEGSTNFAKVSYLRGEADIAPAIDSRSVLMVLHLG
                     PDFSRRLEGGMPAEVQLILDGRRSNAAQIVAGYAQAIFDRFERELAATRRTPPPPSVV
                     TARIWFNPNLETTWNTVPALVAILTTLMGLVVTALSVARERELGTFEQLLVSPLDPHE
                     IVIGKTVPALIIGLTEGTFMLLVGIFVFRVPFQGSFVLLYLAMTVFLAAVIGVGLFIS
                     SLATTQQQGILGAFVFMVPAILLSGFATPIENMPEWLQTLTLANPLRYYVAIIKGVFL
                     KGMPAATVVQLLWPLAVIALATLSAATWLFRHRVE"
     gene            complement(2130..3287)
                     /locus_tag="VHS97_23410"
     CDS             complement(2130..3287)
                     /locus_tag="VHS97_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3451221.1"
                     /translation="MKTAPPDTSGRLRRILGLIRKETLQIVRDPSSYLIAVVLPLLLL
                     FIFGYGVSLDLRNLPVGLVVEQPSPEADSLVASFHNSRYFTIRLARHRSLVEDDLVSG
                     RLKAVVVLAADFAQRLGRGETAPVQVIVDGSDPNTAGLAENYLQGVWDNWLQQEAVSA
                     GGLAVRPGAASRIVAQPRYWFNPQIDSRDFLIPGAVAVNMSLIGTLLTSLVVAREWER
                     GTMEALMATPVGVADFLLGKLIPYFVLGMAAMVLSVIMATTVFRVPFRGSAWALVGVS
                     SAFLAAMLPLGLLISTVTKNQFAASQAALMAAFLPAFELSGFIFEIDSMPAPIRLFTQ
                     ILPARYFVSSLQTLFLAGDVPSVLIPDSLALLTIAAVLNLMLIRSSRMRLE"
     gene            complement(3268..>4360)
                     /locus_tag="VHS97_23415"
     CDS             complement(3268..>4360)
                     /locus_tag="VHS97_23415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3451222.1"
                     /translation="DGPPRELTARVEGRSFLVQGAGAARREVLATALRLPEILDAAIQ
                     GESVRLVVRRGSMPPEPSALGRDGIEITPVAPRFEDAFIDLLGGSSKAESPLAGPGRH
                     RDDGRSVVEAHELTKRFGDFTAADRITFRIGRGEIFGLLGPNGAGKSTTFKMMCGLLR
                     PTFGSARVAGFDLYRAASSARARLGYMAQKFSLYGDLSVRQNLEFFAGIYGLPRDRRR
                     AMTARMVESFGLQPYLGGNAGSLPLGFKQRLALACAVMHEPEVLFLDEPTSGVDPLTR
                     REFWSHINALVERGVTVMVTTHFLDEAEYCDRVALVYRGRIIAKGSPDELKDKARSDS
                     TPEPTLEDAFIALVEAYDRQGPDHEDRAA"
BASE COUNT          866 a         1397 c         1397 g          700 t
ORIGIN      
        1 cgaggcgacc agtgtcgtgt ggggcatgcc gggtgcagcg gtcgccaccg gccaagtcga
       61 tgagatcctg ccgctggacg acatcggtcc caaggtcgcc gcgctcctgg ggaccgggat
      121 accgccatgc aactgacgcc cgccgcattc gacgatatcc gcaaggccgt tcacgaccta
      181 tgcggcatcg tgatcgcccc agataaacaa tacctggtga aagcgcgttt ggagcccatc
      241 ctcttaacca acggccttgc ctcctacgag ttgctcgtgc ggcagctcag agagccgaat
      301 tcggtcttgc tccgcgatca gacggtcgag gccatcacga ccaacgagac ttcattcaat
      361 cgcgacggcc atcccttcga cgcactgcgt agttttatca ttccggagct cgccagccgg
      421 ttgatcgaac ggagagcaac gacacgactc cctcattccc aggcgcgtat ctggtccgcg
      481 gcggtgtcga ccggccagga agcctacagc gtggcgatgg ccgttgccga ttgcctggtc
      541 gcacgatcgg ccccaggcgt gacattggat gattttccaa tcctggcctc cgatatctcc
      601 agcaaggcac tcgcgtttgc ccgcgaggca cgatattcct ccgcggaggt cgaccgcggc
      661 cttagcgccc tcgagagggc tcgattcgtc cgccaggaaa atggagcctg gatcgtcgat
      721 gcggcgctcc gccgcatggt cgaattccgc cgacttaacc ttgtccagcc gctgcccgaa
      781 ctgggaactt ttgacctgat attatgccgg aatctcctga tttatctcga cgacgcgacc
      841 cgccgccgtc tctgccgtgg cctgcactcg gccctcaatc cgcggggaat cctcatgatt
      901 ggcgcggccg agagcctcta tggagtgacc gacgccttcg cgacggagcg tttcgggagc
      961 accgtggtgt atcggaaacc ataagcggcg gacgaacgcc agtcgcaaaa ctcgtctact
     1021 caacccgatg ccggaacagc catgttgccg ccgagagcgt ggcgagcgcg atcaccgcca
     1081 acggccaaag cagttgcacc acggtcgccg cgggcattcc ctttaaaaac acccccttga
     1141 tgatcgccac gtaataccgc aatgggttag ccagggtcag cgtctggagc cactcgggca
     1201 tgttctcgat tggagtagcg aatcccgata gcagaatcgc cggaaccatg aacacaaacg
     1261 cgcctaagat gccctgctgc tgcgtcgtgg ctagcgacga gatgaataga ccgacgccga
     1321 tcacggcggc caggaatacc gtcatggcca ggtagagcag cacgaaggat ccttgaaacg
     1381 gcacgcggaa aacgaagatt ccgaccagga gcatgaacgt cccctcggtc agcccgatga
     1441 tcaaggccgg caccgtcttg ccgatcacga tctcgtgcgg gtcgagcggc gaaaccaaga
     1501 gctgctcgaa cgttccgagc tcacgttcac gggccacgga gagcgccgtc acgactagcc
     1561 ccatcagcgt tgtgaggatc gccaccaggg cgggcaccgt gttccaggta gtttccaggt
     1621 tcggattaaa ccagatccgg gcggtcacga cgctcggcgg aggcggggtg cgccgtgttg
     1681 cggcgagctc ccgctcgaaa cggtcgaaga tggcttgcgc gtagccggca acgatctggg
     1741 ccgcgttcga gcgccggccg tcgaggatga gctggacctc ggccggcatt ccaccttcga
     1801 gccgtcgcga gaagtcgggc cccaagtgca ggaccataag aacgctccgc gagtcgatgg
     1861 ccggcgcgat gtcggcctcg ccgcggaggt agctgacctt ggcaaagttg gtcgagccct
     1921 cgaaccgcgc gatcaggtcg cgcgcggctg tgccgtagtc ttcgttcagg acggccatcc
     1981 gcacgttctt cacctcctgc gtggccgcga cggagaagac gagcatctgg aggatcggcg
     2041 gcacgatgag gatgtaccgc cccctcgggt cgcgcaaggc ggcgagcaac tccttgacaa
     2101 cgagcgtcca gacgcgctgc cacatcgaat cactccagcc tcatccggct cgagcggata
     2161 agcatcaggt tgagcacggc ggcgatggtc aagagggcca gcgagtcggg tatcaagacg
     2221 ctcggcacat cacctgcaag gaagagcgtc tggaggctcg atacgaaata acgcgcgggc
     2281 aaaatctggg tgaagaggcg gatcggcgcg ggcatgctgt cgatctcgaa gatgaagccc
     2341 gagagctcga atgccggcag aaatgcggcc ataagcgcgg cctggctcgc ggcgaactgg
     2401 ttcttcgtca cagtcgagat caacaggccc agaggcagca ttgcggcaag gaacgcggac
     2461 gacacaccca cgagcgccca cgccgagccg cgaaacggca cccggaaaac cgtggtcgcc
     2521 atgatcaccg agagaaccat cgcggccatc cccaacacga agtagggaat gagcttgccg
     2581 agcaggaagt cggcgacgcc cacgggggtt gccatcagcg cttccatcgt gccacgctcc
     2641 cattcgcggg cgacgaccag cgaggttaag agcgtgccga tcaaggacat gttgaccgcg
     2701 actgcgccgg ggatcagaaa gtcgcggctg tcgatctgcg ggttgaacca gtatcggggc
     2761 tgggccacga tccgggatgc ggcgcccggc cgtacggcca gcccgccagc cgatacggcc
     2821 tcttgctgca accagttgtc ccagactccc tgcaaataat tttcggccag gccggccgtg
     2881 ttagggtcgc tgccgtcgac gatgacctgc accggcgccg tctcaccgcg gccgagccgc
     2941 tgggcgaagt cggccgccag aacgaccacg gccttcagcc ggccagagac caggtcgtcc
     3001 tcgactaggg atcggtgccg ggcgagccgg atcgtgaagt agcgggagtt gtgaaacgag
     3061 gccaccaggc tatcggcctc gggcgacggc tgttcaacca ccagtccgac cggcaggttt
     3121 cgcaggtcga gcgaaacccc gtagccaaaa atgaacagca acaaaagcgg tagcacgacc
     3181 gcaatcagat agctgctggg gtcgcggacg atctgcaagg tctctttgcg tatcaggcca
     3241 agaatacgcc gaagccggcc ggacgtgtca ggcggcgcgg tcttcatggt caggtccttg
     3301 ccggtcgtaa gcttccacca gggcgataaa cgcatcctca agtgtcggct ccggcgtgga
     3361 gtcggagcgt gctttgtctt tgagctcgtc cggagagccc ttggcgatga tgcggcccct
     3421 gtagaccaag gcgacacggt cgcaatactc ggcctcgtcg agaaaatgag tggtaaccat
     3481 cacggtgacg ccgcgctcga ccagggcgtt gatgtgcgac cagaactcgc ggcgggtcag
     3541 cgggtcgacg cccgaggtcg gctcgtccag gaacagcact tccggctcgt gcatgaccgc
     3601 gcaggccagg gcgagccgct gcttgaagcc caagggcagc gagcccgcgt tgccacccag
     3661 gtagggctgc aggccgaacg actcaaccat ccgggcagtc atagcgcggc gccggtcgcg
     3721 aggcagacca tagattcccg cgaagaactc gaggttttgc cgcacgctaa ggtcgccgta
     3781 aagggagaac ttctgggcca tgtagcccag ccgcgcccga gcagacgagg cggcacgata
     3841 caggtcgaac cccgccacgc gcgccgaccc gaacgtcggg cgcaagaggc cgcacatcat
     3901 cttgaacgtg gtcgacttgc ctgcaccatt ggggccgagc aggccgaaga tctcgccccg
     3961 gccgatccgg aacgtgatcc ggtcggcggc ggtgaagtcg ccgaagcgct tggtcagttc
     4021 gtgggcttca acgaccgagc ggccgtcatc ccgatggcga ccgggcccgg cgagcggcga
     4081 ttcggctttc gagctgccgc ccaagaggtc gatgaacgca tcctcgaacc gtggcgcgac
     4141 cggcgtgatc tcaatcccgt cgcgcccgag agccgatggt tccggcggca tcgaccctcg
     4201 acgcacgacc agccggacac tctcgccttg gatggcggcg tcgaggattt cgggcagcct
     4261 gagggccgtg gccaagacct ctcgacgggc ggcgcccgca ccctggacga ggaagcttcg
     4321 tccttcgacg cgcgccgtca gctcccgggg tgggccatcg
//
LOCUS       DATAQL010001359         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_291099, whole genome shotgun sequence.
ACCESSION   DATAQL010001359 DATAQL010000000
VERSION     DATAQL010001359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291099"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            714..2393
                     /locus_tag="VHS97_23420"
     CDS             714..2393
                     /locus_tag="VHS97_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3451223.1"
                     /translation="MRRLLAFFLAMLLVAGTSGSVTRAEPPLARPAGSASSAAGKAAA
                     EDAETVLERGLDLERARNWSAAIETYHTALERWPSRVDFGRRLRLCEIHFKITRRYLD
                     TSFRNILLRLPPEQAADLYDEVLERIETNYVDPVPLEPLVRNGLDNLEVALRDAVFVK
                     TNAPAATPERVNLLRDTLRQYRARLSVADRPAAIRLALTSGELARQAIGMNATPVLLE
                     FACGACDALDDFTSYLTPDKLEDLYAMIDGNFVGLGVELKIEKDGLRLVGVIRGGPAW
                     DAGLKIGEVIVKIGGRSIKGLSLDEAANRLQGAEGTTIDLEVRRPDNTVQALRLVRRH
                     VDVESVSKLGMVDQADGIGYLQLSGFQKTSGEEIDQAIDSLVRQGMRVLVIDLRGNPG
                     GLLNVSVEIAERFIDSGLIVSTRGRAQGQSQNLSANGRARWRMPLYVLVDHDSASASE
                     ILAGALQDHQRATILGTRTYGKGSVQSIFSLRSAPAGLKLTTAKFYSPHNRPYSEQGV
                     QPDIPITLRSAAKPTEDGTVPREEQTFGDPQRDPVLATAIRTAKSQLQTAR"
     gene            2612..>3033
                     /locus_tag="VHS97_23425"
     CDS             2612..>3033
                     /locus_tag="VHS97_23425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451224.1"
                     /translation="MPEIGGRPGTRIEPTTTWTVVTDSPLKGLVFAREAGAILAWDEG
                     NQLYLLNARGETHSTSRVPNRILAGSISDEGSLIALLVEAEDAGLLLLSADFDILSER
                     PAPSEASFVTIDPHGRFIALGTRQNAVYVINRFGRPACR"
BASE COUNT          591 a          949 c          910 g          583 t
ORIGIN      
        1 tcgtggccgt gaccctggaa cgggtcaggt aaccacggtt gtcgaaggaa agagccagtc
       61 gcagcccgtg ttgcgcttgt cgggcattca ccatgattca gccgcatgat cggatctcct
      121 gtctcgccat attcgactac ccgttccgca gacagtccgc gtttttgaat ttgtgacaat
      181 cggtctggta agagagacag aaaccaaaat gcccatctga tcagcctaca gcctattttt
      241 tttgcttctt gcccggttat ccgggattgt gagtccggca tcagaatctg cgaagtgtgg
      301 acttaggcgg gaaagccgcg tctcgtgcga ctcgctattc atccggaaaa tcatcaacac
      361 gaagagccaa cttacccttc cccgtgtggt atgattggcg tgaatgcgga tgcgggtcca
      421 aaaccgtccg tattccggac cggcgagttc ggcctcgatt ttgcaagggt cgaggccgtg
      481 aatgtcgtgc ccgctttggc acggaatgac tccatcgaac ggcaacgacg gagcgtggcg
      541 caaggaatct gtggtcgcac cagcgtgcaa ggtttccgga ttcacgacag tcgccgggtt
      601 caaccgactt gatcggcaga agtttcggcc cactccgttg aacggttccc cctcctgcgt
      661 gccggcgacg acgatccgtg gcgcaacgct ccccagcggg aggattgtac gccatgcgcc
      721 ggctgctcgc gtttttcctg gccatgttgc tcgtggccgg gacgtccggc tcggtcaccc
      781 gggcggaacc tcctctcgca cgccctgctg gcagtgcttc atcggccgct ggcaaggccg
      841 cggccgagga tgccgagacc gttttggagc gcgggctcga cctcgagcgg gcacgcaact
      901 ggtcggcggc gatcgagact tatcacacgg ccctcgaacg ctggccgagt cgggtcgatt
      961 tcggacgccg gcttcggctc tgcgaaatcc acttcaagat cactcgccgc tacctcgaca
     1021 ccagttttcg caacatcctg cttcgcttgc cccccgagca agccgccgat ctctacgacg
     1081 aagtgctgga gcgcatcgag accaactacg tagaccccgt accgcttgag cctttggtgc
     1141 ggaacggact ggataacctc gaagtagccc tgcgtgacgc tgtgttcgtc aagaccaacg
     1201 cgccggcggc aacgcccgag cgcgtgaact tgcttcgcga cacgcttaga caatatcggg
     1261 ctcggctgag cgttgccgat cgcccagctg cgattcgcct cgccttgacc agcggcgaat
     1321 tggcccgaca ggccatcggc atgaacgcca cgccggtctt gctggagttc gcctgcggcg
     1381 cctgtgatgc acttgacgat ttcaccagtt acttgacgcc tgataagctc gaagaccttt
     1441 acgccatgat cgatggcaac tttgtcggcc tgggcgttga acttaaaatc gaaaaggacg
     1501 gcctgcgctt ggtcggtgtg atccggggcg gaccggcgtg ggatgccggg ctcaagatcg
     1561 gcgaagtgat cgtcaagatt ggcggccggt cgatcaaagg attgagcctc gacgaagcgg
     1621 ccaaccggct tcaaggggcc gagggaacca ccattgatct cgaagtgcga cgacccgaca
     1681 acaccgtcca ggctctccgc ttggtgcgcc gccacgtcga cgtcgagagc gtcagcaagc
     1741 tgggaatggt tgatcaggcc gatggaatcg gctatctcca gctctccggc ttccaaaaga
     1801 cctccggcga agagatcgat caggccatcg actcgctcgt tcggcaggga atgagggtgc
     1861 tcgtcatcga cctgagagga aacccgggcg gcttgcttaa cgtttcggtc gagatcgccg
     1921 agcgcttcat cgactccggc ttgatcgtgt cgactcgcgg gcgcgcccag ggacaatcgc
     1981 aaaacctctc cgccaacggt cgggcccggt ggcgaatgcc gctgtatgtt ctcgtcgatc
     2041 acgacagcgc cagcgccagc gagatcttgg cgggtgcact acaggatcac cagcgggcaa
     2101 cgatcctcgg cacccgcaca tacggcaaag gctcggtcca gagcatcttt tccctccggt
     2161 cggcgccggc cggcctcaaa ctaaccaccg ccaaattcta ttcgccccac aatcgtccct
     2221 atagcgagca aggcgtccag cccgatatcc cgatcaccct ccgctctgcc gccaaaccaa
     2281 ccgaggatgg caccgtccct cgcgaggaac agactttcgg tgaccctcaa cgagaccccg
     2341 tcctcgcgac cgcaatccgc acagccaaga gccagcttca gacagcgcgg taacagcgaa
     2401 agacataggt aaatccccgc caattcagca tcgaccgggc gaatcgccct gtgcaacaga
     2461 caaagccgag cgtctccgcg cgtgagtgtc gaacggggtg agtcggtcgt ggtacgatcg
     2521 gacacttagg caagagcgcc ctagttgtgt tgccgccaga cattctggca ggcccagatc
     2581 gacagccgcg gcgagcgggg gaagacaagc gatgcctgag atcggcggca ggcctgggac
     2641 gcgaattgag cccacgacaa cctggacggt cgtgaccgat tctccactca aggggctcgt
     2701 attcgcgcgc gaggcgggcg cgatcctcgc atgggacgag gggaaccagc tttacttgtt
     2761 aaacgcgcgg ggtgaaacgc attcgacgtc gcgcgtcccc aatcgcattc tcgcgggctc
     2821 gattagcgac gaaggcagcc tgatcgctct cttggtcgag gcggaggacg ccggtttgct
     2881 gctcttgagt gcggatttcg acatcctgtc cgagcggccc gcgccgtctg aggcgtcgtt
     2941 tgtcacgatc gacccgcacg gtcgctttat cgcgttggga acccgccaaa atgcggttta
     3001 tgtgatcaat cgcttcggcc gtccggcctg ccg
//
LOCUS       DATAQL010001360         5131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1513869, whole genome shotgun sequence.
ACCESSION   DATAQL010001360 DATAQL010000000
VERSION     DATAQL010001360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513869"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VHS97_23430"
     CDS             <1..265
                     /locus_tag="VHS97_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451225.1"
                     /translation="SNGLRQVLDEYVIRPGRRVVAHTLIWSGAIVLMVLGTYAILMFE
                     KDQAYIHARQSARRGEVVSAPEESLVTPADSPRVVRAGRMLTP"
     gene            327..2183
                     /gene="sdhA"
                     /locus_tag="VHS97_23435"
     CDS             327..2183
                     /gene="sdhA"
                     /locus_tag="VHS97_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX3451226.1"
                     /translation="MNNYHRFECVIVGAGGAGLMAALESSRRVGTAVVSKLYPTRSHT
                     GAAQGGIAAALSNLENDSWENHAYDTIKGGDFLVDQTAAEILCREAVECIYELEHMGL
                     PFDRTAEGRISQRRFGGHTKPNPGYDPAAPAGTEAAKPRLPVMRACHSADRTGHMILQ
                     TLYQQCIKQGVTFFDEFHMLDLIMVEGECRGVVALEIATSQLHVFHAKSVLLATGGFG
                     RMFKVSSNAYALTGDGPAIAYRHGLPLEDMEFYQFHPTGIYRLGILLSEACRGDGGIM
                     FNGQGERFMEKYAPNLKDLASRDVCSRSIYREVRAGRGVGGEDFVYLDIRAATMNKYK
                     SSPLHPEKDVDSAWVEKRLPDIIEFARTYLGVDPIHEPMPIQPTAHYAMGGLPTDLDG
                     RIVKDASGTVVRGLYSAGESACVSVHGANRLGTNSLVDLVVYGRRAGKAMAEYARHHD
                     FEHIPPHSEAAAQAAVEGLLSRPRTEPWVRLRDEMQTTMMDNCGVFRTNETLTRARDD
                     IAGLKVRFRNLGVQDKGAVYNTNLLEIIELGNLLDLAEATVAAALSRTESRGAHFRED
                     FPDRNDDAFFKHSLVYSTGDSQTRVDYKDVDILMVEKDGQRVPKYPLEIRKY"
     gene            2254..3045
                     /locus_tag="VHS97_23440"
     CDS             2254..3045
                     /locus_tag="VHS97_23440"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX3451227.1"
                     /translation="MTQIPASSVGSRSAVRHLTVRIKRYDPERDLPPRWQEFKIDANP
                     MDRVLDVLQAIKWNQDESLALRRSCAHGVCGSDAMRINGANALACKTLVKSLEGDVLT
                     IEPILGLPVIKDMIVDMKQFFDHYKSVMPFLVNDEPPPTTERLQSPEQRARFDDTTKC
                     ILCACCTTACPPFWGDKKYVGPASIVNAHRFIFDSRDRAAADRLDILNDVEGVWRCRT
                     VFNCTSACPRDIHVTKAIQEVKQAMLTGSLGDEQASRTIGGDGGR"
     gene            3102..4844
                     /gene="dacB"
                     /locus_tag="VHS97_23445"
     CDS             3102..4844
                     /gene="dacB"
                     /locus_tag="VHS97_23445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX3451228.1"
                     /translation="MAHRSRCRARGGWALFGHRFTLVVVAAIALAQNGAITGQEVSRA
                     KAHDTQARRVESVLATPGFEHGHWGILVVDRKTGQTIYERNPDLLFAPASVAKLFSTA
                     AALIELGPNFRFQTPLYRRGDVDAGGTLHGDLILLAQGDLAMGGRTGPEGKLVFHDDD
                     HTYADGNLRSQVVPVDPLAGLDHIAREVRAAGILEVTGLVIIDDRLFAAAEATGSGPK
                     RVSPIVINDNVIDILARPADKVGEPSIVTFQPATQFMTMDAQVMTVDASKSPSLEVHA
                     VGPRRFAVRGQLPLGHTRVVKIREVDEPGSFARALLIEALGRRGVRVAASPLGHNDTG
                     SLAPRTDVARLPKVAEYTSPPFSEYIRIILKVSHNLHASTLPLLLAARHGERTLAPGL
                     RRQGQILKSLGVEPGTISFGGGAGGARADLASPRATVNLLRVMASRPEFAVYDAALPI
                     LGRDGTLAKAVAQDSPARGHAHAKTGTFYVENELDGTTVLTSKALAGYLETASGRSLI
                     LAAFVNNVPLDAPKPDRPVFEATAAAGRLLGKLCEVFYTDTADDSTVPSRLGPSTADE
                     SPAAKDAKLKSQIH"
BASE COUNT         1000 a         1544 c         1593 g          994 t
ORIGIN      
        1 ctctaacggt ctgcgccagg tgctcgacga gtacgtgatt cgtccgggcc ggcgcgtggt
       61 tgcgcacacg ctgatctggt ccggcgcaat cgttttgatg gtgctgggaa cctatgccat
      121 tttgatgttc gagaaagacc aggcatacat ccacgccagg caaagcgccc ggcgcggcga
      181 ggttgtgagc gctcccgaag agagtttggt cactcccgcg gattcgccac gggtcgtccg
      241 cgcggggaga atgctgacgc cctgaaatat cctgcctccg gccgcggtta tggaatagtc
      301 gcacccttga ttcaagcatt tcaattatga ataactacca tcgatttgag tgtgtgattg
      361 tcggagcggg tggggcgggg ttgatggcgg ccctcgaatc gagccgccgc gtcggcacgg
      421 cggtggtgag caagctgtat cctacgcgct cgcacactgg ggccgcgcaa ggagggatcg
      481 ccgcggcgct ctcgaatctc gaaaacgatt cgtgggagaa ccacgcttac gacacgatca
      541 agggtggtga ttttctggtc gatcagacgg ccgccgagat tttgtgccgg gaagctgtgg
      601 aatgcatcta cgagctcgag cacatgggtc tgccattcga ccgcacggcc gaggggcgga
      661 tctcgcagcg gcgctttggc ggccacacca agcccaatcc gggttacgac ccggccgcgc
      721 cggccggcac cgaggccgcc aaaccgaggc tgccggtgat gcgagcctgc cactcggccg
      781 accgaaccgg gcacatgatc ttgcagacct tgtaccagca atgcatcaag cagggggtga
      841 ccttcttcga cgaattccac atgctcgatc tgatcatggt cgagggcgaa tgccgcggag
      901 tcgtcgcgct cgagatcgcc accagtcagc tccacgtctt tcatgccaag agcgtgctcc
      961 tggcaacagg cggcttcggt cgaatgttca aagtatcttc caatgcctac gccttgaccg
     1021 gcgatggtcc ggcgatcgct tatcgccacg ggttaccgct tgaagatatg gagttttacc
     1081 agtttcatcc gacgggtatc tacaggctgg gaatcctctt gagcgaggct tgccggggcg
     1141 acggcggcat catgttcaac ggccaggggg agcgattcat ggaaaagtac gctcctaacc
     1201 tcaaggatct ggccagccgc gatgtctgtt cgcggtcgat ctatcgggaa gttcgcgccg
     1261 gacgcggggt ggggggcgag gactttgtct acctcgacat ccgcgccgca acgatgaaca
     1321 agtacaagtc gagcccgctc catccggaaa aggacgtcga tagcgcctgg gtcgaaaaac
     1381 ggctgccgga catcatcgag tttgcccgaa cttatcttgg ggtcgaccca atccatgaac
     1441 cgatgccgat tcagccgacg gcgcattatg ccatgggtgg gctacccacc gaccttgacg
     1501 gccggatcgt gaaagatgcc tccggcacgg tcgtacgggg actttattcg gccggcgaga
     1561 gcgcctgcgt cagcgtccac ggcgccaacc gcctgggaac caacagcctg gtcgacctgg
     1621 tcgtgtacgg caggcgggcg ggcaaggcca tggccgaata cgcccgtcac cacgatttcg
     1681 agcacattcc tccccattct gaggccgccg cgcaggccgc tgtggagggg ctactatcgc
     1741 ggccgcgcac cgagccctgg gtcaggctcc gcgacgagat gcagacgacg atgatggaca
     1801 attgcggcgt gttccgcacc aacgagacct tgacgcgagc ccgcgatgac atcgccggac
     1861 tcaaggtccg ctttcggaat ctcggtgtac aagacaaggg tgcggtctac aacaccaacc
     1921 tgctggagat catcgagctc ggtaacctgc tggaccttgc cgaggcgacg gtcgccgctg
     1981 ccctgtcgcg cacggaaagt cgcggagccc atttccgcga ggatttcccc gatcgcaacg
     2041 acgacgcctt cttcaagcat tcgcttgtgt attcaactgg cgattcccag acgcgcgtcg
     2101 attacaagga cgtagacatc ctgatggtcg agaaagatgg ccagcgagtc cccaagtatc
     2161 cgctggaaat tcgtaagtac taagaggccg attcgccgcc ccgaaagaga tcggccggga
     2221 atcgcaacac gatccgatgg aaggcagacg tcgatgactc agatacccgc gagttcagtt
     2281 ggttcccgct cggccgtccg ccacctgacg gttcgtatca aacgatacga tcccgaacgc
     2341 gacctgcccc ctcgctggca ggaattcaag atcgacgcca atccgatgga ccgcgtcctc
     2401 gatgtgttgc aagcgatcaa gtggaatcag gatgagtcac tggccttgcg ccgttcgtgc
     2461 gcacatggcg tgtgcggttc cgacgcgatg cggatcaatg gcgccaatgc cctggcctgc
     2521 aagactttgg tcaagagcct cgagggcgat gtcctcacga tcgagccgat cctggggctg
     2581 ccggtgatca aggatatgat cgtcgacatg aagcagttct tcgatcacta caagtctgtg
     2641 atgccgtttc ttgtcaatga cgagcctccg ccgacgaccg agcgcctgca atcgcccgaa
     2701 cagcgcgcgc gattcgacga tacgaccaag tgcatacttt gtgcttgttg cacgacggct
     2761 tgtcctccat tctggggtga caagaaatat gtcgggccgg cttcgatcgt caacgcccac
     2821 cgtttcatct tcgactcgcg cgaccgcgcg gccgccgacc gactggacat tctcaacgat
     2881 gtcgagggcg tgtggaggtg ccgtacggtt tttaattgca ccagcgcctg cccgcgcgat
     2941 attcacgtga cgaaggccat ccaggaagtc aaacaagcga tgttgaccgg aagcctggga
     3001 gacgaacaag cttcccggac gattggtgga gacggtggcc ggtgatgttt cgccacggtc
     3061 tcgttgactg agacatcgag tgaaaaaggg gtcaacgcga aatggcacac cgaagtaggt
     3121 gtagagcgcg aggcggttgg gcgctatttg gtcatcgttt caccttggtc gttgttgcgg
     3181 ctatagcact cgcgcaaaac ggagcaatca cgggtcaaga agtctcgcgc gccaaggcac
     3241 acgacaccca ggcgcggcgc gtcgagtctg tgctggcgac gcccggcttc gagcacggtc
     3301 attggggcat tctggtcgtc gaccgcaaga ccggccagac catttatgag cggaatccgg
     3361 atctcttgtt tgctcctgcg tcggtcgcca agctgttcag tacggcggct gccctgatcg
     3421 agctagggcc taacttcagg ttccagacgc cgctctatag acgcggcgac gtcgacgccg
     3481 gcggcactct tcacggtgat ttgatcttgc tggcccaagg cgacctggcc atgggtggac
     3541 gcactggccc agagggaaag cttgtattcc acgacgatga ccacacctac gccgatggaa
     3601 accttcgcag ccaggtggtg ccggtcgacc cattggcggg tcttgaccac atcgcgcgcg
     3661 aggtgcgggc cgcgggaatc ctcgaggtaa ccggccttgt gatcattgac gaccggttgt
     3721 tcgctgcggc cgaggcgact ggcagcggcc caaagagagt gagcccgatc gtgatcaatg
     3781 acaacgtgat cgacatactg gcccggccgg ccgacaaggt tggggaaccg tcgatcgtca
     3841 cgtttcagcc ggcgacccag ttcatgacga tggatgccca ggtgatgacc gtcgacgcca
     3901 gcaagtcacc ttcactggag gttcatgcgg ttggtccgcg caggttcgca gtgcgcgggc
     3961 agttgccgct gggccataca cgcgtcgtca agatccgcga agtcgatgaa cctggctctt
     4021 ttgctcgcgc gctattgatc gaggctctag gtcgccgcgg cgtccgggtt gcggcttcgc
     4081 cgctgggcca taacgacacc gggagcctgg cgccgcgcac cgatgtggcg aggctcccca
     4141 aagtggccga atacacatcg ccccctttca gcgaatatat ccgcataatc ttgaaggtta
     4201 gccacaatct gcacgccagc acgttgcccc tgctgctggc agcccgccac ggagagcgga
     4261 cactggcgcc tggactcagg cgtcaaggac agatcttaaa gagcttgggc gttgagccag
     4321 ggacgatctc cttcggcggc ggggcagggg gcgcacgtgc cgatctcgcc tctccccgcg
     4381 caacggtcaa tctcctgcgc gtgatggcat cgcggcctga gttcgcggta tatgacgcgg
     4441 cgctgccgat tctcggtcgt gacgggacgc tcgccaaggc ggttgcccaa gacagccctg
     4501 cccgcggcca tgcccacgcc aagaccggca ctttttacgt cgaaaacgag ttggatggca
     4561 caaccgtctt gaccagcaag gcactcgctg gttatctcga gaccgcgtcg ggccgttcgc
     4621 tgatcctcgc ggctttcgtc aacaatgtac cgctcgacgc ccccaagccc gatcgtccgg
     4681 tcttcgaagc aaccgcggcg gccgggcggc ttttgggcaa actctgcgaa gtgttttaca
     4741 cagatacggc ggacgacagc acggttccgt ccagactggg tcccagcact gccgatgaaa
     4801 gtccggcggc aaaggacgcc aagctcaagt cgcaaattca ttaggcagaa cggtctgggg
     4861 gaacgcgcct cgcggggaaa cgttcttccc ggagtccttc aattcctgct gctgctggtg
     4921 agtctccatt gcgggcagtc gacgccacgt gttttggagt tgatctcctc attttcctcc
     4981 tttgccatca cgacagggtt cggagacgac acgctcgatc attccgcagg ggcttgggtg
     5041 tacgctttct catgaatggg tacgtggggc ttgctcgttg cggcgatccg ccctgaggca
     5101 cgacgcgcgc ccttggaaag gcgcacgacg t
//
LOCUS       DATAQL010001361         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5330079, whole genome shotgun sequence.
ACCESSION   DATAQL010001361 DATAQL010000000
VERSION     DATAQL010001361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5330079"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1439
                     /locus_tag="VHS97_23450"
     CDS             333..1439
                     /locus_tag="VHS97_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451229.1"
                     /translation="MHRRGFTLIELLVVIAIIAVLIALLLPAVQAAREAARRAQCVNN
                     LKQIGLANHNYHQVADSFALGVSLNMYSLPPYWRAKNSWGHFGLLLPFLEQQAVFNAA
                     NFNWGVEEGAPGSSLPIDINVTAADTQLNMFLCPSDPLSGNGGSLSFYNNNDRDTSSY
                     YGCVGATTSLSVPNGVDTGTIQFIPTANFPSTESTGMFSFQKVYGLRDCTDGSSNTIF
                     YSEGVVNPPNTGPKVKNEGVNNVGMDPSARILSAWSNPAAILTAMLLCDQMWQTGGSF
                     DNQKGRDWAHGCMTQTLFNTIVPPNKYRWTHCSNTGSTTMAPFSNAMSYHPGGVNTLF
                     ADGSVRFIKDSIAQNIWWGLGTRNGGEVISSDSY"
     gene            complement(1616..2470)
                     /locus_tag="VHS97_23455"
     CDS             complement(1616..2470)
                     /locus_tag="VHS97_23455"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3451230.1"
                     /translation="MILVTGATGNIGKELVAQLLAEKGQQLRVVSRDERKLLNLDPDV
                     ERLIGDLRERTTVERAVRGVDRLFLVWSLVDESHEADRMLIDEAQRAGVRHVVAISSL
                     ATSLAADRGIGALHREKERFIESSGMSWTFLRPGAFMSNALQWIGTIKSQGKVFSPTG
                     DGQTAPISPYDIAAVAAVALTAPGHEFQIYELTGSELLSAHEQVKILARELGKPIECV
                     DVPSSVAAEGMRKAGLPAFVVDSLAEFWEVARRGGSAIKTDTAKRLTEREPQTFETWC
                     REHRAAFL"
     gene            2743..3342
                     /locus_tag="VHS97_23460"
     CDS             2743..3342
                     /locus_tag="VHS97_23460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3451231.1"
                     /translation="MGQRQPAVDETRTRIIAAARKLIMSRSGFADFSIDAVARKASVA
                     RMTVYYQFKSKVGLLEALLDDIAERGEIKRISGALANPNPRDALAECIAVFGRFWTLD
                     RVVIRRLRAMATLDPDFEKGLRGRDAHRHEKIRILLQALAEKYGRPNPHALGDAVDLV
                     VVLTSFGTFDHLAGRARTPEEVVPVITRLVFQALGLDSE"
     gene            3667..3825
                     /locus_tag="VHS97_23465"
     CDS             3667..3825
                     /locus_tag="VHS97_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451232.1"
                     /translation="MARALGISIFTEADNWIELQTQVRDAVRCHFEEDGRPKFVRLHY
                     VREEVFAV"
     gene            3822..4064
                     /locus_tag="VHS97_23470"
     CDS             3822..4064
                     /locus_tag="VHS97_23470"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX3451233.1"
                     /translation="MKLPRNLSGEDLAKALERLGYQVTRQTGSHLRLTTDQNGEHHAT
                     IPRHDSLRIGTLSSILRDIAEHHGLSKDDLTDSLFQ"
BASE COUNT          859 a         1319 c         1188 g          861 t
ORIGIN      
        1 catgctttaa tgactgtgaa cgcgaatcta attacagcat aattatgatc ttggtgtgtc
       61 acacgtcact cacttgactc ggatccgctg attcgtcatc atgagggatg gataggggtc
      121 ctaaatctct gtcggttcca tttctccctc accgccaacc atacgtaacg acatctgtcg
      181 gcctcacatg ggagccatct tgctctcgga atcagatgtt gagcgaggcc gagatcaaca
      241 acgatgtcgg gattcttgga gctccaatcc cgtcttgctc tgcgcgttac caaggcatgt
      301 tgaacagagg tttcctttcg ggagatgaag atatgcatcg ccgcggattt acgctgatcg
      361 aattgctggt ggtgatcgcg atcatcgcgg tcttgatcgc gctgctcttg cccgccgtac
      421 aggccgcccg cgaggcggct cggcgcgccc aatgcgtcaa caacctcaag cagatcggcc
      481 tggccaacca caactaccac caggtcgccg attccttcgc gttgggcgtc tcgctcaaca
      541 tgtactcgct gcccccctac tggcgggcca agaactcgtg gggccacttc gggttactgc
      601 tgccgttcct cgagcagcag gcagtcttta acgcggccaa cttcaattgg ggcgtcgagg
      661 agggtgcgcc gggaagtagc ttaccgatcg acatcaacgt cacggcggct gacacacagc
      721 tcaatatgtt tctctgcccg tccgacccgc tgtcgggcaa cggcggctcg ctcagcttct
      781 acaacaacaa cgaccgcgac accagcagct actacggttg cgtcggcgcg accacgagtt
      841 tgtccgtccc caacggcgtc gacaccggca ccatccagtt catcccgacg gccaacttcc
      901 ccagcactga atcgaccgga atgttcagct tccagaaggt ctacggctta cgcgactgca
      961 cggatgggtc gtcgaacacg atcttctact ccgagggcgt cgtgaacccg ccgaatacgg
     1021 gcccgaaggt caagaacgag ggggtcaaca acgtcggcat ggatccgagc gcacgcatac
     1081 tcagcgcctg gtccaacccc gccgcgatcc tgaccgccat gctactctgc gaccagatgt
     1141 ggcagacagg cggcagcttc gacaaccaga agggccgcga ctgggcccac ggatgcatga
     1201 cgcagacgct gttcaacacg atcgtcccgc cgaacaagta ccggtggacc cactgcagca
     1261 acaccggctc aacaaccatg gcccccttca gcaacgccat gagctaccac cccgggggcg
     1321 tcaacacgct gtttgccgac ggcagcgtca ggttcatcaa ggattccatc gctcaaaaca
     1381 tttggtgggg cctgggaact cgtaacggtg gcgaggtcat cagctccgat agttattgag
     1441 tattcggcag gaggatcgag ccggcctgcg tcagccagta gagcgagccc gcgaggcagg
     1501 ccgcatgctt tcatcgcgcg ctgtcctctt ttcccgccca ttgtgggcgt ttcgacagcc
     1561 gcgcgtcgca accttcattg cctgaccgcc tgggtcctcg ccggcgaact gccgattaca
     1621 ggaaggctgc acggtgctcg cggcaccagg tctcgaaagt ctgcggctcg cgttcggtca
     1681 agcgcttggc cgtgtcggtt ttaatggcac tcccaccgcg gcgagcgacc tcccagaact
     1741 cagccaaact atcgaccaca aacgcaggta ggcccgcctt gcgcattccc tcggccgcga
     1801 cgctcgacgg cacgtcgaca cactcgatcg gctttccgag ctcgcgtgca aggatcttaa
     1861 cttgctcatg cgcgcttaaa agctcgctgc cggtcagctc gtagatctgg aactcgtgcc
     1921 cgggtgccgt gagggccacg gccgcgaccg ccgcgatgtc gtacggagag atcggtgccg
     1981 tctggccgtc gccggttggt gagaaaacct tcccctgcga cttgatcgtg cctatccatt
     2041 ggagtgcgtt ggacatgaac gcgcctggcc gcagaaatgt ccagctcatg cccgacgatt
     2101 caataaatcg ctccttttcc cgatggagtg ctccaatgcc gcggtccgct gcaaggctcg
     2161 tcgccagcga tgagatggcc actacgtggc gcacccccgc gcgctgggct tcgtcaatga
     2221 gcatccgatc ggcttcgtgg ctctcgtcaa cgagggacca caccaagaac aatcgatcca
     2281 ctcccctgac cgcgcgctcg accgtcgtcc gctcgcgcag atcgccgatc aagcgctcta
     2341 cgtccggatc gagattcaac agcttacgct catcgcgcga cacgacgcgc aactgttggc
     2401 ctttctccgc aagaagctgc gccaccagct ctttgccgat attccccgtc gcgcctgtaa
     2461 cgagaatcat cacacactcc tttagttcat gctcgatgaa agaaattcgg acatccgaat
     2521 ttgtgcaaca accagtgact ccgctgcatc agctaacgaa gcggctacgg cccgcctccc
     2581 aggcggtata atacctgcgg cgccccgaat gtcggccgtt cgtgcgatgt aactcgcctc
     2641 tcggtcgttc gaccccgccc cagccgccat ccaagttatt tgtatagact gtcaaaacaa
     2701 aatgaggttg tcaatcccgc tatgtgccct cgaccttatc gcatgggtca acgtcagccg
     2761 gcagttgatg agactcgcac caggattatc gctgccgccc ggaagttgat catgtcgcgc
     2821 agcggttttg ccgacttctc gattgacgcg gtcgcgcgaa aggcgagcgt cgctcggatg
     2881 actgtctatt accagttcaa gtcgaaggtc ggtctgctgg aggcgttgtt ggacgacatt
     2941 gccgaacgag gcgagatcaa gcggatatca ggtgcgttgg ccaatcccaa tccgcgtgac
     3001 gcgctggccg aatgcatcgc agtcttcggg cggttttgga ctctggaccg cgtggtgatt
     3061 cgcaggctgc gcgccatggc cacactcgac ccggatttcg agaaagggct gcgcggtcgc
     3121 gacgcgcacc ggcacgagaa gattcggatt ctcttgcagg ccctggctga gaaatatggc
     3181 cgtcccaacc cccatgcgct cggtgatgcc gttgacctag tggtcgtgct gaccagcttt
     3241 ggcaccttcg accacctcgc cggccgcgca cgaactcccg aggaagtcgt ccctgtgatc
     3301 acacggctcg tattccaggc actcgggctc gacagcgagt gaagcgatgg ctcgatcgca
     3361 tgggagcaga cgcttgtgta gtcgcaacca aaaacgttac gcggccatcg gaaccgttga
     3421 aaagacccgt tcgccagaga tgccacgcga gagggaacgg tgggcttcga cggccttttg
     3481 tggtacctgg caagacactc gatcgagacg ttgtggtaag attaccgaag cggatgtgcg
     3541 ccgttcttgc tctctgagcc tggatctgac aagagcttat ggtgccgcac cagggacgtt
     3601 ccaagagatt cgcaacctat gactgaattg atattccttg ttgaggaatc cccagacgga
     3661 gggctcatgg ctcgggcgct cggcatttcg attttcactg aggcggacaa ctggatcgag
     3721 cttcagactc aggtccgcga tgcggtgcgt tgccactttg aggaggacgg gcgccccaaa
     3781 ttcgtccggc tccactacgt acgcgaggaa gtcttcgccg tatgaagctg ccgcggaatc
     3841 tctcgggtga agatctggcc aaagccctcg agcgactggg ttatcaggtc acacgtcaga
     3901 ccggctcaca cctacgcttg acgaccgatc agaacggcga gcaccacgcg acgattccac
     3961 gccacgattc attgcgcatc ggaacactgt cgtcgattct gcgcgatatt gccgaacacc
     4021 acggattgag caaggacgac ctgaccgatt cattgttcca gtaggataac ttacggatcg
     4081 acgaggagca gtcggaacat gcctatggcc ccgcagcgaa gtcgacagat tgccgcattc
     4141 cctttgtccg tcttctgcct tggcgtcgtc ggctgaccaa gagttggtgc gtacgacagt
     4201 tggttcaaag atcaagccta gctttca
//
LOCUS       DATAQL010001362         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1970751, whole genome shotgun sequence.
ACCESSION   DATAQL010001362 DATAQL010000000
VERSION     DATAQL010001362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970751"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VHS97_23475"
     CDS             <1..1151
                     /locus_tag="VHS97_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3451234.1"
                     /translation="IDPHAGQICYAVTVADVNGDQKPDVVAVTEDAVVWYENPSWQKH
                     DIIRKVTARDNVCIQPHDVDGDGRIDFALGAGWRPPDTKNPSTLQWLGRDKSGIWQVH
                     PIRYQEPTLHRLRWGDVKGTGKKQLVVAPLQGRGTKGPNWGEGDGVRVLAYDVPADPG
                     TEIWPVEVAESSLHTAHNLQILDIDGDRQDEIVLACWEGVFVLDRSPEGNWTKTRIGT
                     GNQDSVPSKGSSEVKVGRLHARKSYVATIEPWHGYQVVVYTDQNQNSSTPAVPQLWSR
                     QVIASPVQWGHAVWTANLDDDDDEELLIGQRDPNKPGTAGPRGPGLFVFDPKPGSNPL
                     EFERYTIDDGGMACEDAVAADLDGDGRADLVAGGRATHNVRIYWNRGK"
     gene            1234..1959
                     /locus_tag="VHS97_23480"
     CDS             1234..1959
                     /locus_tag="VHS97_23480"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3451235.1"
                     /translation="MIVPAVSAALIILAVTGGWTQTAGTGTKTDPKINEPFKNPDLKG
                     FIKKFESTEREVYAKRKDIVAALELKPGMAVADVGAGTGLFTRLFADKVGPSGKVYAV
                     DIAPDFLKHIAADAAKRGQTQVVTVVGSQDSINLQRASVDLVFLCDVYHHLENPERNL
                     SSIRRALRERGRLVVIDFDRVEGKSTEFVLKHVRASQDVFRREIEAAGFKLSPLAKLP
                     PLTETFFLRFEKAASARNTSNEK"
     gene            complement(2001..2333)
                     /locus_tag="VHS97_23485"
     CDS             complement(2001..2333)
                     /locus_tag="VHS97_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451236.1"
                     /translation="MHFGDEEEVDHVDPPAVPATDLADGFRRIAGRSNPPPPIFHPLA
                     YRGVVAEWPVEWRERWGRRANALEESGLSWRDAEAQAFVEVWKEIRREQPAESATPNV
                     TSSEPARN"
BASE COUNT          528 a          700 c          752 g          450 t
ORIGIN      
        1 aaatcgaccc gcatgcgggc cagatctgct atgcggtgac ggtggccgac gtgaacggcg
       61 atcagaaacc cgacgtggtc gcggtgaccg aagacgcagt tgtctggtat gaaaatccga
      121 gctggcagaa acacgacatc attcgcaagg taactgcccg cgacaacgtc tgcatccagc
      181 ctcacgacgt cgacggtgat ggccgaatcg atttcgctct cggagccggc tggcgcccgc
      241 cggatacgaa gaatcccagc acgctgcagt ggctcggtcg ggacaaatcg gggatctggc
      301 aggttcatcc cattcggtac caagaaccca ccctgcaccg cctgcgctgg ggcgacgtca
      361 aggggacggg caaaaaacag ctcgtcgtcg ctcccttgca aggccgcggc acgaagggtc
      421 caaactgggg ggaaggtgac ggggtccgcg tactcgctta tgacgtgccg gccgaccccg
      481 gcactgagat ctggcccgtc gaagtcgccg aatcgtcgtt gcacacggcg cacaatctgc
      541 aaatccttga catcgacggc gatcgtcagg acgagatcgt cttggcgtgc tgggaaggag
      601 tcttcgtact tgaccgcagt ccggagggga actggacaaa aacccgcatt gggacgggca
      661 accaggattc cgtgccgtcg aagggatcga gtgaagtcaa ggtcggcagg ctacacgccc
      721 gcaaatcgta tgtcgcgacg attgagccgt ggcacggcta tcaggtcgtc gtttacaccg
      781 atcaaaatca aaattcgtcg acgccggcgg tcccgcaact ctggtcgcgg caggtgatcg
      841 cctcgccagt gcaatggggg cacgccgtct ggaccgccaa ccttgacgac gatgacgatg
      901 aagaactgtt gatcggccag cgcgacccca acaagcccgg gacggcggga ccgcgaggcc
      961 cgggtctatt cgtgttcgac cccaaacctg gctcaaaccc gcttgaattc gagcgctaca
     1021 ccatcgacga tgggggcatg gcgtgcgaag atgctgtcgc cgccgacttg gatggcgacg
     1081 gacgtgcgga cttggttgcc ggaggacgag ccactcataa tgtgcggatc tattggaacc
     1141 gaggcaaata gcgagcggcc agaaggtttc gggcgtaaaa cgtgtgacga gtaggagggt
     1201 ttgaggatgg atcgactatg cgttcatcgt ctcgtgatcg tcccggcagt gagtgcggcg
     1261 ttgattattc tggcagtaac cgggggatgg acccagacgg ccggtaccgg aaccaagacc
     1321 gatccgaaaa tcaacgaacc tttcaagaac ccggacctca agggttttat caaaaagttt
     1381 gaatccaccg aacgcgaagt gtatgccaag cgaaaggata ttgttgcggc tctggagttg
     1441 aagccgggca tggctgtcgc ggacgtcggg gcgggaacgg ggctgttcac tcggttgttc
     1501 gccgacaagg tggggccgag cggcaaggtt tacgccgtcg atatcgcgcc tgatttctta
     1561 aaacacattg ccgcggacgc cgccaaacgc ggccagacac aggtcgtgac cgtggtggga
     1621 agccaggact cgatcaatct gcagcgagcc tctgtcgacc tggtttttct ctgcgacgtc
     1681 taccaccacc tggaaaaccc ggagagaaat ctgtcttcga tccgtcgggc cctcagggag
     1741 cggggaaggc tcgtggtgat cgatttcgac cgggtggaag gtaaaagtac ggagtttgtt
     1801 ttgaagcacg tgcgggccag tcaggacgtg ttccgaaggg aaatcgaggc ggccggattc
     1861 aagctttcgc cgctggccaa gctgcctccg ctcacggaga ctttttttct tcgcttcgaa
     1921 aaagccgcat ccgcccgcaa tacatcaaat gaaaaatgac gagttgcgcc cacaaggtcg
     1981 cgcaaccgtc attgggcgaa ttaattcctc gcgggctccg acgacgtgac attgggtgtt
     2041 gccgattcgg cgggttgttc gcggcgaatt tccttccaga cctctacaaa tgcctgagct
     2101 tccgcgtcgc gccacgagag gccactttcc tccagcgcat tggcgcgccg gccccaacgc
     2161 tcacgccact ccacaggcca ttccgccacg acgcctcgat aggcgagtgg atgaaaaatg
     2221 ggtggcggag gattggatct gccagcaata cgacggaaac cgtcggcaag atccgtcgca
     2281 gggacggctg gaggatccac gtggtcgacc tcctcttcgt ccccgaaatg catcgagagc
     2341 cttccttgga cacaccgaac gcgagaccgt tctcggcctc ttctcagggt ccgaagctcg
     2401 ctcacaggaa acccctaact tccccgttgt
//
LOCUS       DATAQL010001363         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3496166, whole genome shotgun sequence.
ACCESSION   DATAQL010001363 DATAQL010000000
VERSION     DATAQL010001363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3496166"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VHS97_23490"
     CDS             <1..1683
                     /locus_tag="VHS97_23490"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3451237.1"
                     /translation="AIIAMAQPRWGRLAASPLPPGHDVVLLVDVSRSMAVEDAVPNRL
                     AAAVEAAESLVNALAREASNRVAVVAFAGRGVKHCPLTENLGAVLDALHRLRPGVVRP
                     GGTDLGAALDAAREALGPEQHAEGRAIVIFSDGEDHADAWSSRLDRLRQEDIVVHAVA
                     IGDLDQGHPVPDGKTAETIQYHGEQVLSRRIDLALESVARRTDGSIVKLGLASGDLGT
                     LYRTKIEPAARRRRESTRLADPAERFPLLLIAALTSLLAGCWPARRGWSWPWNWYWTW
                     SWRRSLAKAGTAVLVVIMAGLATGAGDSPARVESASAAEAVARGKSAYDLRRWEEALA
                     EFQTAIARAPASPVPRYNAGASLYQLGRYSEAHQRYLEASERADLYLRTKIDYAIGNT
                     ALAEGDIPGAIRSYDECLASTARGAALDAIRRDAAINRSFALEQPQSLAAPQDDRSGD
                     GSQSRKPDRKRSSKTPGAGEKQSSETEPESDPANAGANGENDTRGDRNRPPASRRRMG
                     GAGGGRTTPPEARGDSPDDRLDAALEHIRAAQSNRIPDDEPPVSANDDRKDW"
     gene            1754..3073
                     /locus_tag="VHS97_23495"
     CDS             1754..3073
                     /locus_tag="VHS97_23495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451238.1"
                     /translation="MRRQPRASIGILGFALALLIGLYPGGRTASQEADRPRVQAEVAP
                     GPYHVGQGFELLVGVFAKGKEPKIDLPEIKGARAWTIGTELRPISSTGIGRVVAQEYQ
                     YVVKFRVVAGRAGPLEIPAIQAQVGGRSGRSQPKRLLVKPVPVQGRPAEFLGGVGRFE
                     LSAEASPKTVRVGQELDFGIKVTGPAAWGMSGRPDLARYERPELGLRITAGPDQTSDE
                     PPVRTFVYRLRPARAGDMVLPPVSIASFDPALARYVTHVTAGVPVRVVAVPSFDPTTI
                     PDESLSAGAGWPSRIIWAVTAAIPVLLGAYLMLRHVRRRLERATAPYPELARRYAKRS
                     VLESTSPEMISNTAVRIAERLSTYMKLGLGRPMGALTPDEAQEGVVRLTGSVELGDQA
                     GRLTMRCDTILYAETTREAEDGRELLDDARRLFDALGRVKYSNRSGV"
     gene            complement(3070..4296)
                     /locus_tag="VHS97_23500"
     CDS             complement(3070..4296)
                     /locus_tag="VHS97_23500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3451239.1"
                     /translation="MIRRVLEEPTVGFQSMGLSTTMLGALKQVRYVTPSPIQAAFIPQ
                     ALDGFDVIGQAKTGTGKTAAFGIPIIEMLEARGRGPQAIILAPTRELTQQITGELQRL
                     TFGGDVVVCGTFGGEPIERQIRALTRGVDIVVGTPGRVLDHIERRTLYLGDIVHVVLD
                     EADRMLDIGFRPDIERILRKLPTPRQTLLLSATINADVRKLAQRYMFEPLEVNLSQDE
                     PSVDTIQQFYISVNTDRKFDLLLLLLKRDRPRQCIVFTRTKRGADRLADRLSRVIKGV
                     SAIHGDLPQTTRDRVMRGFRSGQISVLVATDVVGRGIDVEGISHVINYDMPDDAENYV
                     HRIGRTGRMGKDGVAYLFVCPDQGEPLTAIENLINRVIPPLSIEGFGKPRPRAEPAAR
                     ELFNTAATRTHALAVR"
BASE COUNT          873 a         1388 c         1537 g          853 t
ORIGIN      
        1 gcgatcatcg ccatggctca gccgcggtgg ggacggttgg ctgcgtcgcc actaccgcct
       61 gggcacgatg ttgtcctcct ggtcgacgtc agtcggagca tggcagtcga agacgcggtt
      121 cccaatcggc tggccgcggc ggttgaggcg gccgagagcc tggtgaatgc cctggcgcgc
      181 gaagcgagca accgcgtggc cgtcgtggcg ttcgccgggc gaggggtcaa gcattgtccc
      241 ttgacggaaa acctcggtgc cgtactcgac gcgttgcaca gactgcggcc cggagtcgtg
      301 cggccggggg gcacggacct cggtgcggcc ctcgatgcgg ctcgcgaggc tctcggtcct
      361 gaacaacacg ccgaagggag ggccatcgtc attttctccg atggcgaaga tcatgccgat
      421 gcctggagct cgcgactgga ccggcttcgt caggaagata tcgtggtcca cgccgtcgcg
      481 atcggcgacc tcgaccaagg tcatcccgta ccggatggca agacggccga gaccattcaa
      541 tatcatggcg aacaggtcct ctcgcgccga atcgacctgg cgctcgagtc cgtggcgcgg
      601 cggacggacg gatcgatcgt gaagctcgga ttggcgtcag gcgatctcgg tacgctctac
      661 cggaccaaga tcgagcctgc cgctcgtcgg cgtcgtgagt cgacgaggct ggctgacccg
      721 gcggaacgat ttcctttgct cttgatcgcc gcgctcacat cccttctggc cggttgctgg
      781 ccggcgcggc gcggatggag ctggccgtgg aactggtatt ggacttggag ctggcggcgt
      841 tcgctcgcca aggcgggcac ggccgttctg gtcgttatca tggccggtct ggcgacgggc
      901 gccggcgaca gcccggcgcg ggtcgaatcg gcgtccgcgg ccgaagcggt tgcccggggc
      961 aaatccgctt acgatttacg ccggtgggag gaagccctcg cggagttcca gacggcgatc
     1021 gcgcgcgcgc ccgcttcgcc cgtgccgcgc tataacgcgg gagcatcgct ttatcaactg
     1081 gggcgctata gcgaggccca tcagcgttat ctcgaggcca gcgagcgggc cgacctttat
     1141 ttgcggacca agatcgatta cgcaatcggc aacaccgcac ttgccgaagg agacattccc
     1201 ggggcaatcc gctcctatga tgagtgcctg gcctcgaccg cgcgaggcgc ggctttggac
     1261 gctatccgca gggatgccgc gatcaaccgc agcttcgcgc tggaacagcc gcaatcactt
     1321 gccgctcccc aagacgatcg ctccggcgac ggctcgcaat cacgaaagcc cgatcggaaa
     1381 cgaagctcga aaacgccggg cgccggcgaa aagcagtctt ccgagacgga accggaaagc
     1441 gatccggcga acgccggtgc caacggtgag aacgatacgc gcggcgatcg caaccggccc
     1501 cctgcgagtc ggcgccggat ggggggcgcg ggtggcggac ggactacccc gccggaagca
     1561 cgaggagact cgcccgacga tcggctcgac gctgcgcttg agcacatccg ggccgcccag
     1621 agcaatcgca ttcccgatga cgagccccct gtgtccgcca acgacgatcg taaggattgg
     1681 tagattgatc ggacagagta gccgaaaccc gaggaattct gagcgccgcg acctttccca
     1741 atctcgcgaa atcatgagaa gacagccacg tgcgtcgatc ggcatccttg ggttcgccct
     1801 ggcgcttttg atcgggctct atcctggtgg gcgtacggct agccaggaag cggatcgccc
     1861 gcgcgtgcag gccgaggttg cgccaggacc ttaccacgtg gggcagggtt tcgagctctt
     1921 ggttggcgta tttgccaagg ggaaagagcc caagatcgat ctgccagaga tcaaaggcgc
     1981 gcgggcctgg acgatcggca cggaactgag accaatcagt tccacgggca ttggcagagt
     2041 ggtggcgcag gaataccagt atgtcgtcaa gtttcgcgtg gtcgcggggc gtgccggtcc
     2101 gctcgagatt ccggcgatcc aggcccaggt cgggggccgt tccggacgca gccagccgaa
     2161 gcgcctgttg gtcaaaccgg tgccggtaca gggacggcca gccgagtttc tggggggggt
     2221 aggccgattc gaactatcgg ccgaggcatc gccgaagacc gtgcgggtag ggcaggagct
     2281 cgatttcggg atcaaggtga ctgggccggc cgcctggggt atgtctggtc gtcccgatct
     2341 ggcgcgatac gagcgcccgg agctcggcct gcggatcacg gctggccccg atcagacgag
     2401 cgatgagcca ccagttcgga cgtttgttta ccgccttcgt ccagcgcggg cgggcgacat
     2461 ggtcttgccg ccggtgtcga tcgcctcgtt cgacccggcg ctcgcccgtt atgtgaccca
     2521 cgtcacggcg ggcgtgcccg tccgcgtcgt cgcggttcca tcgttcgatc ccacgacgat
     2581 ccccgatgaa tcgctctccg cgggggcggg ctggccgagc cgaatcatct gggccgtgac
     2641 tgccgcaata cccgttctac tcggggcgta tctgatgcta aggcacgtgc ggcgcaggct
     2701 tgaacgcgcg actgcgccct atccggagct ggcgcggcgc tatgcgaaac ggtcggttct
     2761 agaaagcaca tcgccggaaa tgatttcgaa caccgccgta cggatcgcgg agaggctgag
     2821 cacttacatg aaactcgggt tgggacggcc gatgggcgca ttaacgccgg atgaagccca
     2881 ggaaggagtg gttcggttga cgggttcggt ggaactcggc gatcaggccg gccggctcac
     2941 catgcgatgt gacacgatac tctatgcgga aactacgagg gaggccgaag atgggcgtga
     3001 attgctcgac gacgcccgaa ggctgtttga tgccctcggg cgggtcaaat attcgaaccg
     3061 cagtggcgtc tagcgaacgg ccaaggcatg agtgcgcgtg gcggcagtgt tgaaaagctc
     3121 gcgggccgcc ggctcggctc tggggcgggg tttgccaaag ccctcaatcg agagcggcgg
     3181 tatgactcgg ttgatcaggt tttcgattgc cgtgagcggc tcaccctgat cggggcagac
     3241 gaacagatat gcgacaccgt ctttacccat tcgtccggta cggccgatgc ggtggacgta
     3301 gttctcggca tcgtcgggca tatcgtagtt aatcacatgg ctgatgccct cgacgtcgat
     3361 gccgcgaccg acgacgtcgg tggcgaccag aacggaaatc tggccgctgc gaaatccgcg
     3421 catgacgcgg tcgcgcgtgg tttggggaag atcaccatga atggccgaaa cacccttgat
     3481 caccctgctc agacgatccg caagccggtc ggccccccgc ttggtgcggg tgaagacgat
     3541 gcactgcctt ggtcggtcac gcttgagcaa aagaagcagc aggtcgaatt tccggtcggt
     3601 attgacggaa atatagaact gctggatcgt gtcgaccgat ggctcatctt gggaaagatt
     3661 gacctcgagc ggctcgaaca tgtaacgttg cgcgagtttt cgcacatcgg cgttgatcgt
     3721 ggcggacagc agaagcgttt ggcgcggcgt gggcagcttg cgaaggatcc gttcgatatc
     3781 agggcggaag ccaatgtcga gcatccgatc ggcctcatcg agtacgacgt gcacgatatc
     3841 gccgagatac agcgttcgtc gctcgatatg gtcgagcaca cggcccggcg tgccgaccac
     3901 aatgtcgaca ccgcgcgtaa gggcgcggat ttgacgctcg atgggctcac cgccgaatgt
     3961 cccgcaaacg acgacgtcgc ccccgaaagt cagccgttgc agctcgcccg taatctgctg
     4021 agtcaactcg cgggtcggcg ccaggatgat cgcctgcggc cctcgtcccc tggcttcgag
     4081 catctcgatg atcggaattc caaaggcggc cgttttgccg gtgccggttt tggcttgtcc
     4141 gatgacatcg aatccatcga gcgcttgggg aatgaatgca gcctgaatgg ggctgggtgt
     4201 aacgtagcgg acttgcttca gggcgccgag catcgtcgtg ctcagcccca tggactggaa
     4261 ccccacggtg ggttcctcaa gaactcgtcg aatcaatcaa ccctccagaa acttttccta
     4321 aacgaatcgc tgacaacatc ttgactacca aagatcttat ccgtttaacc cacattcgtc
     4381 aaaggcaata cagccagagc cgcgcgaatg catcaatttc tctcggatgt gatgcggctg
     4441 gagaatcttg acggccgcac aagcagtatt gagacgctga gattgctcgt ctcttggctt
     4501 gctcacactg acgaccgcgc gctactctct atgtttgtag gaggcaacgg aaggtgggat
     4561 cggtttctat ttacgcctcg gtcgattcaa ctcccgaggc tcccccacga aacgactagt
     4621 gttcgcagga taatgtcggg cccaagaaga t
//
LOCUS       DATAQL010001364         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5482331, whole genome shotgun sequence.
ACCESSION   DATAQL010001364 DATAQL010000000
VERSION     DATAQL010001364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5482331"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VHS97_23505"
     CDS             complement(<1..828)
                     /locus_tag="VHS97_23505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX3451240.1"
                     /translation="MAKSRSLIFEYLESRIVRAAIGLPWRDPLHLTLSFAPDGTSIAA
                     NSSSLFQDLDARFPSIVAWQDDIVRAFQTWAAETNISLGLVADNGAPFGTAGLMQGDP
                     RFGDIRIGARPMSPEVMAITVPPDPYMSGTLSGDMILNSAADLNPGNLFDVALHEAGL
                     ALGLGETTDPSSVMYPVINPRAALSPTDIQNIQTLYGTRAPDPNNNTITTATPISQPL
                     LYLGLTPLVAYGNLAAPSNTDFYSVQPPSLYAGPVTVQLQTSGISFLQPEVQVFNQHF
                     "
     gene            complement(938..>2437)
                     /locus_tag="VHS97_23510"
     CDS             complement(938..>2437)
                     /locus_tag="VHS97_23510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3451241.1"
                     /translation="LLGSDIPDAEHEQRESGMALARETMDRYRVMESPRWQEAPLVNA
                     LVPEQREQVRDDMGELLLLLAGAVAQQTQLDFALRLNDLAAGCYPADAVPCAIWRQRA
                     ELVRSAGQSDEAQRLLIKCEAAPVRSPRDRYLLLLTVYRQRGRFPEALPWLKEASRCQ
                     KDNFSVWLILGNCYAALGKRNDAVECYDMAAALWPESHWPSMCRGLAFLEQGNDRRAL
                     AAFDEVIRLRPETLPAYHNRAVAKYHLGDLAGARADLTHLLSNPKPPLRGYFLRAKVR
                     AKEGDREGARRDQEDGLRGEPRDERDLTARGLVRQPRDPRAALADYESALKLNPHYLT
                     ALQNKANVLGEDLRRTEEAVATLDTLLAFFPNYVPALAGRGVLYARLGRREAALADAR
                     EALPKDSKPYNVYQVAGIYALTSRQNSDDRREALRLLESALSQGFGLDVLEKDRDLDA
                     IRDQPEFRRLVEGARARRTKAAPRVTPPRTAVERGGRAESSGQSNAPSG"
BASE COUNT          412 a          752 c          810 g          463 t
ORIGIN      
        1 gaagtgctga ttgaagactt gcacttctgg ttgtaggaag ctgatccccg aggtctggag
       61 ctgaacggta accggcccag cgtagagcga gggaggctgc accgagtaga agtcggtatt
      121 cgagggggcg gcaaggttgc cgtacgctac cagcggcgtc aatccgaggt agaggagcgg
      181 ctgactgatc ggggtggcgg tggtgatcgt gttgttgttt gggtccgggg cgcgggtgcc
      241 gtagagcgtc tggatgttct gaatgtcggt cggcgacagt gcggcccggg gattgatcac
      301 gggatacatg accgaacttg ggtctgtggt ctcacccagc ccgagcgcga ggcccgcctc
      361 gtggagcgca acgtcgaaca ggttgccggg gttcaagtcc gccgcactgt tgagaatcat
      421 gtcgcccgac aacgtccccg acatgtaagg gtccggcggc acggtgatgg ccatgacctc
      481 cggcgacatc ggccgcgccc cgatccggat atctccgaag cgcggatcgc cctgcatcag
      541 tccggcggtg ccgaacggag caccattgtc ggccaccagg cccaacgaaa tattggtctc
      601 cgccgcccag gtctggaacg cgcggacgat gtcgtcttgc caggccacga tgctggggaa
      661 tcgggcgtcg agatcctgaa acaagctgct cgagttggcg gcgatcgatg tgccgtcggg
      721 cgcgaagctg agggtaaggt ggagggggtc tcgccagggc agtccaatgg ccgcccggac
      781 gatccggctt tccaaatact caaagatgag cgatcttgac ttggccatgg cttcctcccc
      841 gaagcgtttg cccgtcgatg ggcggtcaat agtggcttcg attacgtgct acttcgagtg
      901 tgtggtcatc ggtggaaacg tattcaattc tcagagctca tccggaaggg gcattggatt
      961 gtccgctgct ttcggcccga ccgcctcgtt cgaccgctgt acgcgggggc gttacccgcg
     1021 gtgcggcctt ggtgcggcga gctcgcgccc cctcgaccag acgccggaac tcgggctggt
     1081 cccggatcgc gtccagatcg cgatccttct cgagcacgtc caagccgaat ccctggctca
     1141 gggccgattc tagcaaccgc agggcttccc ggcggtcgtc tgagttttgg cgcgacgtca
     1201 gagcgtagat gccggcgacc tgatagacgt tgtacggttt cgaatccttc ggcagcgctt
     1261 cgcgggcgtc ggcaagcgcg gcttcacggc ggccgagccg agcgtacagt accccccggc
     1321 ccgcaagcgc cggcacatag ttcggaaaga aagcgaggag cgtgtcgagt gtcgcgacgg
     1381 cctcttcggt gcgcctgagg tcctctccga gaacgttcgc cttgttctgg agggccgtta
     1441 gatagtgggg attgagcttg agtgcgctct cgtaatccgc caaggccgcc cgcgggtcgc
     1501 gcggttgccg taccaagcct cgcgccgtca agtcgcgttc gtcgcgcggt tccccacgca
     1561 atccgtcctc ttgatctcgg cgggcgccct cgcggtcccc ttccttcgct cggactttgg
     1621 ccctcagaaa gtagccgcgg agcggtggct taggattgct cagcaggtgc gtcaagtcgg
     1681 cccgcgcccc ggcgagatcg cccaggtggt acttcgcgac ggcgcgattg tgatacgccg
     1741 gcaaggtctc gggccgcagc cggatgactt cgtcgaaggc agcgagggcc cgccgatcgt
     1801 ttccctgttc caagaaggcc aagcctctgc acatggacgg ccagtgggac tctggccaca
     1861 aggcggcggc catgtcgtaa cactccaccg catcgttccg cttccccagg gcggcataac
     1921 aattcccaag gatcagccac accgagaagt tgtccttctg gcatcggctg gcttccttca
     1981 accaaggcag ggcttccgga aagcgcccac gctgccggta taccgtcagc aggagcaggt
     2041 atcggtcgcg gggcgatcgc accggggcag cctcgcactt tatcagaagt cgctgggctt
     2101 cgtcagactg gccggccgaa cgaaccagct cggcgcgctg tcgccagatc gcacatggga
     2161 cggcgtctgc ggggtagcaa cctgccgcca ggtcgttcag ccgcagcgcg aaatcgagct
     2221 gagtttgctg cgcgaccgct cccgccagca gcaaaagcag ttcgcccatg tcgtcccgga
     2281 cttgttccct ctgctccggt acaagtgcgt tcaccagggg agcctcctgc cagcggggcg
     2341 attccataac ccgatatcga tccatggtct cgcgggccag ggccatcccg gattcacgtt
     2401 gctcgtgttc ggcgtcgggg atgtcggatc ccaggag
//
LOCUS       DATAQL010001365         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3496417, whole genome shotgun sequence.
ACCESSION   DATAQL010001365 DATAQL010000000
VERSION     DATAQL010001365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3496417"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VHS97_23515"
     CDS             complement(<1..585)
                     /locus_tag="VHS97_23515"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3451242.1"
                     /translation="MSLRINSEAPNFTAETTQGVIDFHEWIGNGWAILFSHPKDFTPV
                     CTTELGYMAGLKPEFDKRNCKIIGLSVDPVDNHSRWAKDIEETQGHAVNYPMIGDPQL
                     KVVKLYDMLPAEAGDASEGRTAADNATVRSVFVIGPDKKIKAMLTYPMSSGRNFDEVL
                     RLLDSIQLTAKQPVATPVNWRQDDDVIIVPSLSDE"
     gene            complement(733..951)
                     /locus_tag="VHS97_23520"
     CDS             complement(733..951)
                     /locus_tag="VHS97_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451243.1"
                     /translation="MSRIMLISSLAALLAASGTHNAKAIQSADQAAWAQDDLACADVG
                     IDPGSAVFSQCVADLRHSLWAEQNLYKS"
     gene            complement(1649..2494)
                     /locus_tag="VHS97_23525"
     CDS             complement(1649..2494)
                     /locus_tag="VHS97_23525"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX3451244.1"
                     /translation="MRNRCPSDGPPLDLRSDPLQSGSPDYLTGLIIDGTILPLPADQA
                     WATGQFNRVPIINGTVEDEGGFGATLNEYFSGQPLTAAGYTGLVTGIYTGNTNFFNGP
                     GGIAGVPPFYPAGTDTKVLARYPLTAYASPSVAEIAILTDTESGRARHLNHLLSQWVT
                     LYTYEFRDRNAPWYFPPLSFPHGAAYTIDLQFFFPLWHGGPLGTPHPLTTQELKLSDE
                     LVAAWTSFMHSGDPDFVGNHPWPRYDADSPAYLSENVPRLSTLSESDFAAAHQCAFWD
                     GILAF"
     gene            2587..>2817
                     /locus_tag="VHS97_23530"
     CDS             2587..>2817
                     /locus_tag="VHS97_23530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451245.1"
                     /translation="MALVDRALALNPSFARGWFISGVLRLYAGQPEIAIEHAEASLRL
                     SPRARVGWALLTIGAAHFYTRHFEKAVPKLLLA"
BASE COUNT          535 a          833 c          899 g          550 t
ORIGIN      
        1 ctcgtccgag agcgacggga cgatgatcac gtcatcgtct tgcctccagt tgaccggggt
       61 ggcgaccggc tgtttggcgg tcaactggat cgaatcgagc aaccggagca cctcgtcaaa
      121 gtttcgcccg ctgctcatcg gataggtcag catcgccttg atctttttgt cgggtccgat
      181 cacgaacacc gagcgtacgg tggcgttgtc agcagcggtc cgcccttcag aggcgtcgcc
      241 cgcttcggca ggcagcatgt cgtaaagctt gaccaccttc agttgggggt cgccgatcat
      301 cgggtaattc acggcatgac cctgggtttc ctcgatatcc ttcgcccagc gcgagtggtt
      361 gtcgaccgga tcgacgctta gcccgataat cttgcaattg cgcttatcga actccggctt
      421 cagccccgcc atgtagccga gctcggtcgt gcagaccggc gtgaaatcct ttgggtgaga
      481 gaataggatc gcccatccgt tgccgatcca ctcatggaag tcgataacgc cttgggtagt
      541 ctccgcagtg aagttcggag cttcggagtt gatcctcaaa gacatggcac ctctcctcat
      601 cgtttgattg tctgattgtc gaacgggatc gctcgaatgt cgaacgactg cctgtcggtt
      661 ataaatagtc gggcgcgtat tgcgagcaag gtctgcgaat ccgaaacccg cagactgctc
      721 gcaagaccac agtcaggatt tgtacagatt ctgctcggcc cacagggaat ggcgtaaatc
      781 cgcgacgcac tggctgaaga cggcgctgcc tgggtcgata cccacatcgg cgcaggccag
      841 atcgtcctgc gcccaagcgg cttgatcggc gctctggatg gccttggcgt tgtgggtgcc
      901 actcgctgcg agcagggcgg caagggagga aattaacatg atgcgtgaca tggcactctc
      961 ggatcttagg ggctatcagg ttaggcggtg cttcgttttg ccggacgggg acggttgccc
     1021 gatggccgtc ggcattcagg gagttcgtgc ggtgtttacg acgcacattg cgaaagttgg
     1081 cgtaccgggc tggagcccct tctgggcaaa tgcgctgcgc tggctcgcaa ccagtcgctg
     1141 ctgatcccat ccggacaggc ttctatcgag gctcctgacg caggtgtcat agcgatcgcc
     1201 agatggatcg agccccaaga cgacggcaca ggcgtggtgc tcctgcgccg ttcgcgacga
     1261 tttctctgcc gcacgggcag aggtgtgagc ggaggcgagc agagcgacgg ccgccgcacc
     1321 gacgatcaat ctcgacaccc gagtgcgacg actgaagcaa ccggatggat cacaagcttg
     1381 cgaatgggtg atgttggaca taacctttcc cctcatggtt cgattgctaa acgggtcaga
     1441 gattgcgccg aacggttgcc gtctccagac cgaattttgt tcattcgttg ctcagttcgc
     1501 cgaggtaacg agggcgatgc cgggctatga tgagcggacc tgcgcggtcg cccgcaaact
     1561 cacccgccgc ggcccgccgg gatcctacgc tggtgcgccg aaggccaggc cgtcgacgcc
     1621 gcagcgctta agggggcagt cgtcgcggtc agaaggccaa gataccgtcc cagaacgcac
     1681 attggtgagc ggcggcaaaa tccgactcgc tgagcgtcga caatctgggc acgttctccg
     1741 acaagtaggc tggcgagtcg gcgtcgtagc gtggccacgg atggttcccg acgaaatccg
     1801 gatcgccgct atgcatgaag ctcgtccagg cggcgaccag ctcgtccgag agcttcaact
     1861 cttgagtggt gagcgggtgc ggcgttccca aaggtcctcc gtgccacagc gggaaaaaga
     1921 actggaggtc gatcgtgtag gctgcgccgt gcgggaagct cagcggcgga aaataccagg
     1981 gcgcgttgcg gtcgcggaat tcatatgtgt agagcgtcac ccattgggac agcaaatgat
     2041 tgaggtgccg ggcccggccc gattccgtgt ccgtcaatat cgcaatctcg gccacgctcg
     2101 gactcgcgta agcagtgagc gggtagcgcg ccaaaacttt cgtgtccgtt ccggcaggat
     2161 aaaacggggg gacgcccgct attccaccgg gaccattgaa aaaattggtg ttgccggtgt
     2221 aaatgccggt gacgagcccc gtgtaccccg ctgccgtcag cggttgcccg ctgaagtatt
     2281 cgttgagggt cgcgccgaaa ccaccctcgt cctcgacggt tccgttgatg atcggcaccc
     2341 gattgaactg gccggtagcc cacgcttggt cagccgggag cggtagaatc gtgccgtcaa
     2401 taatgaggcc ggtcagataa tcaggactgc cgctctggag cgggtcggag cgaagatcca
     2461 gcggcggacc gtctgaaggg caccgatttc gcacggcgag cgctggaagt agccggcgac
     2521 gacccggcta tcctggcgaa cgccgcctac acgctgggct atctcggcga agacatcggc
     2581 gccacgatgg cgctggtgga ccgcgcgctg gcgctcaacc cgagcttcgc gcgcggctgg
     2641 ttcatcagcg gcgtcctgag attgtacgcg ggccagcccg agatcgcgat cgagcacgcc
     2701 gaagcttcgc tgcggctcag cccgcgcgcc cgggtcggct gggctctcct cacaataggc
     2761 gccgcgcatt tttacactcg acacttcgag aaagcagtcc ccaagctcct gctcgcg
//
LOCUS       DATAQL010001366         4661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2581211, whole genome shotgun sequence.
ACCESSION   DATAQL010001366 DATAQL010000000
VERSION     DATAQL010001366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581211"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="VHS97_23535"
     CDS             <1..1807
                     /locus_tag="VHS97_23535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451246.1"
                     /translation="YDVPEAKIDLIAHGIPDMPFVDPNFYKDQFAVEGKFVALTFGLL
                     SPNKGIEQMLRAMPAILEEFPNFVYIVLGATHPNLLREQGERYRISLERLAKDLGIKP
                     NVSFYNRFVEIDELIEFIGMADIYITPYLNPAQIISGTLAYAFGCGKAVVSTPYWYAE
                     ELLADGRGVLVPFGDSEALARETCELLRDEPRRHSMRKKAYMLGREMIWSHVAHLYME
                     SFQRARRSRLDVPYKPLAVRTLAEQPMDLPGWRLDHLVRMSDSAGMLQHANSTIPNFA
                     EGYCTDDNARALLLTVTLEQLGQSTAQVYRLATTYAAFLNYAFDRTRSRFRNFLGFDR
                     NWLEKVGSDDSHGRALWVLGACVGRSRRRDLQFWASQLFDLALPTITETRSPRAWAFG
                     LIGVCLYLERFSGARPATQVRDVLTDRLTECHEKIATDRWPWFEDVLTYDNAKLPHAL
                     IASGRSSGSDQTVELGLQTLQWLVEQQKSPTGCFRPIGSNGFYQRDRERARFDQQPIE
                     ANSTVSACIEAYHATEDAGWLKEARLAFEWFLGANDLGFDLYDAKTGGCCDGLQEDRL
                     NQNQGAESTLAFLLALGEMKLLESTLATYRQVQVS"
     gene            1804..3309
                     /locus_tag="VHS97_23540"
     CDS             1804..3309
                     /locus_tag="VHS97_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEX3451247.1"
                     /translation="MNQIMTIAGESPDGHRHKLNVRRTGIVLKPNNSRVVIRPFEPTD
                     EHRVERIVSRVMSLSDQDVDLMVEDVMREFHNRHQKTRHFFLHRFEQVRKYLITNLPL
                     GENRRMLIGAYFTQEYALESAALFNPSMVWHADQSGLPEGSRRFVLSLRATGEGHISS
                     ITFRSGTIDAGNRIRMDEPTRFVTAPEQVPNTLYDKNLFFRKLLELGVDGPFVGKVLG
                     TLKDQFTLEEMQRAASRAVHQSRPRHHENEPAVQGMLTLAKANYEISYSPEQSISERV
                     IFPSSPTEANGIEDARFVQFRDDDGSVKYYATYTAFDGKVILPQILETDDFLRFNVST
                     LNGPEVRNKGFALFPRKVNGLYAMLSRQDNENIYLMYSDMLHFWYTKELIAKPTYSWE
                     FVQLGNCGSPLETEAGWLVLTHGVGPMRKYAMGAFLLDIDDPSRVIGRLETPLLEPDA
                     NEREGYVPNVVYSCGAVIHNRELVIPYAMSDYASTFATVSVDEILGAMVRD"
     gene            3741..>4661
                     /locus_tag="VHS97_23545"
     CDS             3741..>4661
                     /locus_tag="VHS97_23545"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3451248.1"
                     /translation="MRSWDIGSRADCDVVVDSSLVSARHCQLTQTADGYFLNDLGSTN
                     GTYVNGLRVTAPTRLVPGDSITLGKTMPMPWPPELVRVVQVGRLAGNDVVVDDQRVSG
                     HHAQLVMIEGFETRIEDRGSSNGTFLNSADRRVTGPTTITEADTIYFGTLDVPVARLL
                     DGLREHKTTAPAFAPAPAAVAEPIPEPTAAPPLVADRKRYRWLVVWLVQAPVFAFLIV
                     LLFGRDIATPITMASWAAVENGIASTSFALALAAIWLGCSLAVAATATGPLPVPRVGD
                     IPTNRFVPIGKRLAVLVSLCALGCAVLFAIA"
BASE COUNT          891 a         1462 c         1374 g          934 t
ORIGIN      
        1 gtacgacgtg ccggaagcaa agattgacct gatcgcccat ggcatccccg acatgccctt
       61 tgtcgatcct aacttttata aagaccagtt cgcggtcgag ggcaaatttg tggcgctcac
      121 gttcgggctg ctctcgccca ataagggcat cgagcaaatg ctgagggcga tgccggcaat
      181 cctcgaggag tttcccaact ttgtgtacat cgtcttgggg gcaactcacc ccaacttgct
      241 ccgtgaacag ggtgaacgat accgcatcag cctggaacgg ctggccaagg acctgggcat
      301 caaacccaac gtcagcttct acaaccggtt cgtcgagatc gacgaactga tcgaatttat
      361 cggcatggcc gacatctaca tcacccccta tctgaaccca gcccagatca tttccggaac
      421 tttggcgtac gccttcggct gcggtaaggc ggtcgtctcg acgccgtact ggtatgccga
      481 ggagcttttg gccgatggcc gaggcgtact cgttccgttc ggtgattccg aggcccttgc
      541 acgagagact tgcgagctgt tgcgcgacga gccccggcgc cattcgatgc gaaagaaagc
      601 ctacatgctg ggccgcgaga tgatctggag ccacgtcgcc catctgtaca tggagtcgtt
      661 tcagcgggcg cggcggagcc ggctggacgt accttacaag ccgctggcgg tccgcacctt
      721 ggccgagcaa ccgatggacc tccccggctg gcggctcgac catctggtgc ggatgagcga
      781 ttcggccggc atgctccagc acgccaactc gaccatcccc aattttgcgg aaggctactg
      841 cacagatgac aatgcccgcg cattgctttt gaccgtcaca ctggagcagc tcggtcagag
      901 cacggcacag gtctatcggc tggcgacaac ttacgccgca tttctcaact acgccttcga
      961 tcgcacgcgg agccggtttc gcaatttcct gggcttcgac cgcaactggc tcgaaaaggt
     1021 cggctcggac gactctcacg gccgggctct ctgggtcctg ggggcctgcg tcggccggtc
     1081 gcggcggcgc gatctgcagt tctgggcgtc gcaactcttt gacctggcgc tacccacaat
     1141 caccgagaca aggtccccgc gggcctgggc gtttggcttg atcggcgtgt gcctttatct
     1201 cgagcgattc agcggcgccc ggccagccac ccaggtgcgc gacgtcttga ccgatcggct
     1261 caccgaatgc cacgagaaga tagcgaccga caggtggccc tggttcgagg acgtcttgac
     1321 ttatgacaac gccaagctgc cccatgcctt gatcgccagc gggcgctcca gcggtagcga
     1381 tcagaccgtg gagctgggcc tacaaacgct gcaatggctc gttgaacagc aaaaatcgcc
     1441 gaccggttgt ttccggccga tcggttccaa cggcttttat cagcgcgatc gcgagcgggc
     1501 ccgtttcgac cagcagccga tcgaggctaa ctcgaccgtc tccgcctgca tcgaggccta
     1561 ccatgccacc gaagacgcgg gctggctcaa agaggcccgt ctggcgttcg agtggttctt
     1621 gggcgccaac gacctcgggt tcgatctcta cgatgccaaa accggcggct gttgcgatgg
     1681 tctccaggaa gatcgcctca accagaacca gggggccgag tcgaccctgg cgttcctgct
     1741 ggctttggga gagatgaagc tcttggaaag cacgctggcg acttaccggc aggtgcaagt
     1801 cagttgaacc agatcatgac catcgccggc gagagcccgg acgggcaccg tcacaagctg
     1861 aacgtgcgca ggacggggat cgtcctgaag cccaacaata gccgggtcgt gatccggcca
     1921 ttcgaaccga ccgacgagca tcgggtcgaa cggatcgtct cgcgggttat gtcgctttcc
     1981 gaccaggacg tcgacctcat ggtcgaggac gtgatgcgcg agtttcacaa ccgccatcaa
     2041 aaaacccgtc acttcttcct gcaccggttt gaacaggtca ggaaatacct gatcaccaac
     2101 ctgcccttgg gcgagaatcg ccgcatgctg atcggcgcat actttaccca ggagtacgcc
     2161 ctggaatcag cggctctgtt caatccgtcg atggtctggc acgccgacca gtcgggtctg
     2221 ccggaaggct cgcgccggtt tgtgctcagc ctgcgcgcca ccggcgaggg gcatatctcg
     2281 tcgatcacct tccgtagcgg cacgattgac gccggaaatc gcatccgcat ggacgagccg
     2341 acgcggttcg tgaccgcgcc cgaacaggtg cccaatacgc tttatgacaa gaacttgttt
     2401 ttccgcaagt tgctcgagct gggagtcgac ggtccgttcg tcggcaaggt cttgggcacg
     2461 ctcaaggacc agttcacact cgaagagatg cagcgcgccg ccagtcgcgc cgtccaccag
     2521 agccgaccac ggcaccacga gaacgagccg gctgtccagg gcatgctcac cctggccaag
     2581 gccaattacg agatcagcta ttcccccgag caaagcatct ccgagcgcgt catttttcct
     2641 tcatcgccca ccgaagccaa cggcatcgaa gacgccaggt tcgtccagtt tcgcgacgac
     2701 gacggctcgg tcaagtacta tgccacctac actgcctttg acggcaaggt gatcttgccg
     2761 cagatccttg aaactgacga cttcctccgc ttcaacgtca gcacattgaa cggaccggag
     2821 gtccgaaaca agggcttcgc gcttttcccg cgtaaggtca atgggctcta cgccatgctg
     2881 tcgcggcagg acaacgagaa tatctacctg atgtactccg acatgctcca tttctggtac
     2941 acgaaggagt tgatcgccaa gccgacctac tcatgggaat ttgtgcagct cggcaattgc
     3001 ggctctccgc tggagaccga ggccggctgg ctggtattga cccatggcgt cggcccgatg
     3061 cgcaagtacg cgatgggagc gttcctgctc gacatcgacg acccatcgcg cgtgatcggc
     3121 cggctcgaga cgccgctgtt ggagcccgac gccaacgagc gcgaaggcta cgtccccaac
     3181 gtcgtctaca gttgcggggc cgtcatccac aaccgcgaac ttgtcatccc ctatgccatg
     3241 tccgattacg ccagtacatt cgcaaccgtc tcagtcgacg aaatcttggg cgcgatggta
     3301 cgggattgat aaactgtggc ggatcggtaa gtcgacgagg ctcgcgctca ttcggttcaa
     3361 ttggcataag cgtataagtt catagcaagt tataatcccc tcccctcttg tgggggaggg
     3421 ccagggtggg gggcgttcgc attgcggacc gcagccgaat ccattttccg ccaactgcct
     3481 ccccccaccc caaccctccc ccacgagggg ggagggagtc ggactgacat cggcataacg
     3541 aattacatct aacttagccg tgctggggct ggcgcaccgg agctcagcag gctggcctcg
     3601 agctccacga atgcgatgtc aggtttggag tctcagaggt gcgcgatcgt cgtctggcac
     3661 gcactcgacg aacgcaggcg gttcctgtag cctgacttac gaatactcat ccgggttttc
     3721 gggttggcgg aagggcatct atgcggtcgt gggatatcgg ttccagagcg gattgtgacg
     3781 ttgtcgtcga ctcgtcgctg gtgtccgcgc ggcactgtca attgacccag accgccgatg
     3841 gctatttcct caatgacctg gggtcgacca acgggactta tgttaacggc ttgcgagtca
     3901 ccgcgccgac ccgcttggtc cctggcgact cgatcacgct gggcaagacg atgccgatgc
     3961 cgtggcctcc ggagctggtc cgtgtcgtcc aggtcgggcg cctcgcgggc aatgacgtcg
     4021 tggtcgatga tcaaagagtc tcgggccacc acgcacagct cgtgatgatc gagggattcg
     4081 agacccggat cgaggatcgg ggatcatcga acgggacgtt tctcaattcc gccgatcggc
     4141 gtgtgaccgg cccgaccacg atcaccgaag ccgatactat ttattttgga acgctcgacg
     4201 ttcccgttgc tcgacttctg gacggcctcc gcgaacacaa gacgaccgcc ccggctttcg
     4261 ctcctgctcc cgccgctgtc gcggagccga tacccgagcc caccgccgcg ccgcccctag
     4321 tcgccgaccg gaagagatat cgctggctcg tggtctggct ggtgcaagcg ccagtattcg
     4381 cgtttctgat tgtgttgctt ttcggccgcg atatcgcgac accgataacc atggcaagct
     4441 gggccgcggt cgagaacgga atcgcctcca cgtcgtttgc cctcgcactg gcggcgatct
     4501 ggttaggttg ctcactcgcc gttgccgcca cggcgaccgg accattgcct gttccgcggg
     4561 ttggtgacat cccgacgaat cgcttcgttc caattggtaa gcgactcgcc gtgctcgtct
     4621 cgctttgcgc cctcggatgt gcggtgcttt ttgcaatcgc t
//
LOCUS       DATAQL010001367         5002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4871686, whole genome shotgun sequence.
ACCESSION   DATAQL010001367 DATAQL010000000
VERSION     DATAQL010001367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4871686"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VHS97_23550"
     CDS             <1..743
                     /locus_tag="VHS97_23550"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3451249.1"
                     /translation="SLDVYESEGIDVSHIRILEGVASGVALIFVDETGENMIGVASGA
                     NLKLTPEDVDRLPSSLFRTGDVLLASLEIPVETSIRALRRGIAARMRTILNPAPAPSL
                     SRSTTKELLSVATVITPNRVEALALAGWTPEAGVEPDWAACGSRLSEMGPAAVVITLG
                     SRGCQVIAGKPRLVPAPQVQAVDTVGAGDAFNGALAVAIAEGRPLEEAARWANAAAAL
                     AVTQHGAQAALGFREAIDQLATREMDAN"
     gene            780..2507
                     /gene="ggt"
                     /locus_tag="VHS97_23555"
     CDS             780..2507
                     /gene="ggt"
                     /locus_tag="VHS97_23555"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3451250.1"
                     /translation="MANRAEIAHCYLIIAVACLTVLNLGIDRRAEAGDRPSGPLHKTR
                     SSVMARHGMAATSQPLATEAAIHVLRQGGNAVDAAIAANAVLGVVEPMSCGLGGDLFA
                     IVWDNKTKKLYGLNASGRAPAAATIELFKAKGLTAIPTHGPLSWSVPGCVDGWDQLRK
                     RFGTRPWPELLAPAIDYAETGFPVSEIIAADWLSAEPSLRVIPSSAACFLPSGHAPEK
                     GTIFRNPSLARSLRAIAQGGRDAFYQGPLAEAIVRYSTTVGGLFSHSDFSEHTSNFID
                     PVSTSYRGYDVWELPPNGQGIAVLQMLNLLEPFDLKGMGALSADALHLMIEAKKLAYE
                     DRAKYYADPDFAQLPIAALISKPYAARRRAELKLDQANLSPTAGEPAGADTIYMTVVD
                     GDFNCVSLIQSNFLGFGSYHVPTDLGFALQNRGSLFALDPKHLNRLEPRKRPFHTIIP
                     GFVTKAGQPWLSFGLMGGDMQAQGHAQMICNLIDFGMDVQDAGDAPRFRHLGSSEPTG
                     QPAQGGGSVALESGISAETRRQLEARGHRLVESVGGFGGYQAIRIDLEHGVLIGGSDP
                     RKDGCAMGY"
     gene            complement(2552..3397)
                     /locus_tag="VHS97_23560"
     CDS             complement(2552..3397)
                     /locus_tag="VHS97_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX3451251.1"
                     /translation="MLSPLHSRMRMRFLVGVLALTTIGLGTAGRTQEIPSFAREVPRI
                     RTGEPIFRFNGKNLTGFYSYLRGHKYDDPAKVFTVQNGMIRISGEEFGGLATKDEFHD
                     YHLVAEWKWGEQTFGTRKTNARDSGILLHCVGPDGAAGGNWMESQECQIIEGGCGDLL
                     MVGGRERPRLACETRVGSDGQPYYQKGGKSVTRNSGRYNWWGRDPGWKDILGFRGSRD
                     VEKPTGEWNRMEVICDGDSITNIVNGYVVNIGTQSSLTKGKIMVQSEGAEIFFRTIEI
                     RPLIK"
     gene            complement(3440..3718)
                     /locus_tag="VHS97_23565"
     CDS             complement(3440..3718)
                     /locus_tag="VHS97_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451252.1"
                     /translation="METPQVRPGLVKHAEKMPRVVVFFPNSALGNLAIQLLMQIGIPN
                     DKLGVTPPEQIEGGQGMILSIACPDEKKLAQVESICRSQGGRIHRQRH"
     gene            3944..4852
                     /locus_tag="VHS97_23570"
     CDS             3944..4852
                     /locus_tag="VHS97_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451253.1"
                     /translation="MPTEIAIVTICLALMADPFPLERLGWLDEKLIPEASGIVKSRRY
                     PGIFWVHNDSGNPPLLFAIKGDGRLVRQFRLGVPNIDWEDIAIDDQGHLYVGDIGNNA
                     GALPLRAIYRIDEPDPGSPADEPLAASAMTFYTLPEENRFDAESLFVDRGSATLVAKY
                     HDGREAELFAVPLEPPSPLLRPARPRSIGRLPEFTEPATGADLSTDQTLLVVCASAVT
                     RVYRRDDVNSPAWRLLAVVRYAALPIEGIAWDSRDIALVAEGGGIYRLSEKTWRSALP
                     ARSEPAAPSRSHGQLRDKGGKGLESR"
     gene            4852..>5002
                     /locus_tag="VHS97_23575"
     CDS             4852..>5002
                     /locus_tag="VHS97_23575"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3451254.1"
                     /translation="MAEAVDVVIVGGGVIGLSIALALAREGVRPTVLDRREMGREASW
                     AGAGLI"
BASE COUNT          911 a         1544 c         1535 g         1012 t
ORIGIN      
        1 ggtcgctcga tgtttacgaa agtgaaggga tcgacgtcag ccacatccgg atcttggaag
       61 gagtcgcgtc gggcgtggcg ctcatcttcg tcgacgagac cggcgaaaac atgatcggcg
      121 tcgcgtcagg cgccaacctc aagctaaccc cggaagatgt cgaccgcctg ccgtcttcac
      181 tctttcgtac aggcgacgtg ctcttggcga gcctggagat tcccgtcgaa acgtcgattc
      241 gcgccttgcg gcgcggaatc gcggcgagga tgcggacgat cttgaatccg gcacctgccc
      301 cgtcactctc ccggtctacg acaaaagagt tgctgtcggt cgcgaccgtg atcacgccca
      361 atcgggtgga ggcgctcgcg cttgccggct ggacgcctga ggcaggagtc gagcccgact
      421 gggcggcttg tggttctcgc ctgagtgaaa tgggccctgc ggcggtcgtg atcacgctgg
      481 ggtcgcgcgg ctgccaggtc atcgccggca agccacgatt ggttcccgcg cctcaagtgc
      541 aggccgtcga taccgttggc gccggcgacg cgttcaacgg tgcgctggcg gtcgcgattg
      601 cagagggccg gccgctcgag gaagcagccc ggtgggcaaa cgccgccgcg gcgctcgcag
      661 tgacgcaaca cggagcgcag gcggctctgg ggtttcgcga ggcgatcgac caactggcga
      721 cgcgcgagat ggacgcaaac tgatgtagcc tgactcatcc gaatcgaagg aacctggtaa
      781 tggccaatcg agctgaaatc gcccactgtt acctgatcat cgccgtggcc tgtctgacag
      841 tgctgaatct cgggatcgac cgccgcgcgg aggccggcga ccggccgtcg gggccgctgc
      901 acaagacacg ctcgtcggtg atggcacgtc acggaatggc cgcgaccagt caaccgctcg
      961 ccacggaggc cgccatccac gtcttgcgac aagggggcaa cgcggtggat gccgcgatcg
     1021 ctgccaatgc cgtcttgggg gtggtcgagc cgatgtcttg cggactcggt ggcgacttgt
     1081 tcgccatcgt ctgggataac aagaccaaga agctctacgg tttgaatgcg agcggccgcg
     1141 ctccggccgc cgccacgatt gaactgttca aagccaaggg attaacggcc attccgaccc
     1201 acgggccact gagctggtct gttcctggat gtgtcgatgg ctgggaccag ctcagaaagc
     1261 gctttggaac caggccgtgg cccgaattgc tcgcaccggc gatcgactac gcagagaccg
     1321 gttttccggt gagtgagatc atcgccgccg attggctgag tgccgagccc agcctgaggg
     1381 tgataccctc ctcggccgca tgcttccttc cctccggtca tgcgcccgaa aaagggacga
     1441 tctttcgcaa tcccagcctc gcccggtctc ttagagcgat cgctcagggc ggccgcgatg
     1501 cgttctatca ggggcctttg gccgaggcta tcgtacggta ctccacaaca gtcggcgggc
     1561 tcttctccca ctccgatttc tccgaacaca ccagcaattt tatcgatcct gtctcgacca
     1621 gttaccgcgg ctacgacgtc tgggaactcc cgcccaacgg tcaagggatc gccgtcctcc
     1681 agatgctcaa cctgctcgag ccctttgatc tgaagggaat gggggcgctc tcggccgacg
     1741 ctctccatct gatgatcgag gccaaaaagc tggcctacga ggatcgggcc aaatactacg
     1801 ccgaccccga ttttgcccag ctaccgatcg cggccctcat ttccaaaccg tacgccgccc
     1861 gtcggcgcgc tgagctcaaa ctagaccaag ccaatctgag ccccacagca ggcgagcccg
     1921 ctggagccga cacgatttac atgaccgtgg ttgatggcga cttcaactgc gttagcctta
     1981 ttcagagtaa cttccttggc ttcggttcgt accacgtccc gaccgatctg ggctttgctc
     2041 ttcaaaaccg cggttcgctt ttcgcgctcg atcccaagca tctcaatcgg ttggagcctc
     2101 gcaagcggcc tttccacacg atcattcccg gcttcgtcac caaggccggg cagccatggc
     2161 taagcttcgg tttgatgggg ggcgacatgc aggcccaagg gcacgcccaa atgatttgca
     2221 atctgatcga cttcggcatg gatgtgcagg acgctggtga tgcgccccgg ttccgccact
     2281 tgggctcatc cgagccgacg ggtcagccgg cgcaaggggg tggatccgtc gctctggagt
     2341 cgggtatctc cgccgaaact cgacgtcagc tcgaggcccg gggacatcgc ctggtcgagt
     2401 cggttggtgg attcggcggc tatcaagcca ttcgcatcga tttagagcat ggtgttttga
     2461 tcggtggttc cgacccgcgc aaggacggct gtgcaatggg ctactaaaaa ccgcgctagc
     2521 gaccacggtg aacgtgtcaa aagccttccc gtcacttgat caggggccgg atctcgatcg
     2581 tgcgaaagaa gatctcggca ccctcggatt gaaccatgat cttgcccttg gtcaggctgg
     2641 actgggtgcc gatgttgacg acgtagccgt tgacgatgtt ggtaattgaa tcgccgtcgc
     2701 agatcacctc catgcgattc cattctcccg tcggcttttc gacgtcgcgg ctcccgcgga
     2761 agccgaggat gtctttccag cctgggtctc ggccccacca gttatatcga cccgagttgc
     2821 gggtgacgga cttgccgcct ttttggtagt agggctgacc gtcggatccc accctcgtct
     2881 cgcaggctag ccgcggtctt tcccgaccgc cgaccatcag caggtcgcca catcctcctt
     2941 cgatgatctg gcattcctgc gactccatcc agttgccgcc ggctgcgccg tcgggcccga
     3001 cacaatgaag caagatcccg gagtcacgcg cgtttgtctt gcgagttcca aaggtctgct
     3061 cgccccattt ccattccgcg accaggtgat aatcgtgaaa ctcgtctttg gttgccagcc
     3121 cgccgaattc ctcacccgaa atgcggatca tgccgttttg gaccgtgaag accttggcag
     3181 gatcatcgta tttatgtccg cgcagatagc tgtaaaaccc cgtcagattc ttgccgttga
     3241 accggaagat tggctcgccg gttcgtatgc gcggcacctc ccgagcgaaa gagggtattt
     3301 cctgggtccg gcccgcggtg cccaacccta tcgtagtcag ggcgagaacg ccgaccagga
     3361 aacgcatacg catccgacta tggagtggac tcaacatcaa gcggactctc cgggtgtggc
     3421 gcggcgctca aggtcgacct caatgacgct ggcggtggat ccttccaccc tgagagcggc
     3481 agatcgactc gacctgggcc aattttttct cgtcagggca ggcgatcgat aggatcatgc
     3541 cctggccgcc ttcgatctgt tccggaggtg tgacgcccag cttgtcgttg gggatgccga
     3601 tctgcatcag gagctggatg gccagattgc cgagggcgga gttcgggaag aagaccacga
     3661 cgcggggcat tttttcggcg tgcttaacca acccaggccg aacttggggc gtttccatgg
     3721 acaagccact ctctcgtcga atcgatgcgg cggtatattc ggctgccgcg ttccacgtat
     3781 tcagtaattc gcagccaagt ttgacgtaca agatcgcaaa tcgcaagcag gttccgttca
     3841 cggagtgccc cttgtgatca cccgcgatcc gtggccaatc ccagtacgga cctggtagta
     3901 tgcaagttca tccgagatca acgcgcggga gccccctcga ctcatgccta ccgaaattgc
     3961 gatagtcaca atttgcctgg cattaatggc ggatccgttt cccctcgaac gtttgggctg
     4021 gctcgacgaa aagctcattc ccgaggcgtc gggcatagtc aagagccggc gatacccggg
     4081 catcttttgg gttcataacg actccggcaa ccctcccctg ctcttcgcga tcaagggcga
     4141 cggccggctc gtccgccagt ttcgcttggg agttcccaac atcgactggg aagacatcgc
     4201 aatcgacgac cagggacatc tttatgtcgg ggacattggc aacaacgccg gcgctctgcc
     4261 gttgcgcgcg atatatcgca ttgacgagcc cgacccgggg tcgcccgcgg acgagccgct
     4321 ggccgcttcg gccatgacgt tttatacctt gcccgaggaa aaccggttcg acgcggagag
     4381 cctgtttgtc gatcgaggat ccgcgacctt ggtggcgaag tatcacgatg gtcgtgaagc
     4441 cgagctgttt gcagtgccgc ttgagccccc gtcccccttg ctccggccgg cgcggcctcg
     4501 atcgatcgga cgattgcccg aattcacaga gcctgcaacg ggcgccgatc ttagcaccga
     4561 tcagacgttg ctagttgttt gcgcgtcggc agtgacgcgc gtgtaccggc gtgacgacgt
     4621 gaattcgccg gcatggcgac ttctggccgt agtacgttac gcggccttgc caatcgaggg
     4681 gatcgcctgg gacagccgtg acatcgcctt ggtcgccgag ggtggcggga tttatcgcct
     4741 ctccgaaaag acctggcggt ccgcactgcc ggctcggagc gagccagctg cgccgtcgcg
     4801 ttcgcacggg cagcttcgag ataagggcgg caagggtttg gaatcaaggt gatggccgag
     4861 gcggtcgatg ttgtgatcgt aggcggcggt gtgatcggcc tgtcgatcgc tcttgccctt
     4921 gctcgcgagg gagtccggcc gacggtactc gatcgccgcg aaatgggccg cgaagcgtcg
     4981 tgggcgggcg ccggcctgat tc
//
LOCUS       DATAQL010001368         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1514596, whole genome shotgun sequence.
ACCESSION   DATAQL010001368 DATAQL010000000
VERSION     DATAQL010001368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514596"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1148
                     /gene="pstC"
                     /locus_tag="VHS97_23580"
     CDS             174..1148
                     /gene="pstC"
                     /locus_tag="VHS97_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX3451255.1"
                     /translation="MSPLSTSAENPGGAPAEAALWAGHSHFLAFWEKAVTVGLVICAL
                     ITVFTTAGIIVVLGTQSLEFFARAKLNLTDFLFGTVLKPDAAPPSFGIVPLLWGTFLV
                     AAGSSLIALPIGLLSAIYLSEYAPRSVRSVLKPALELLAGVPTIVYGYLALLLVTPML
                     KRLMEPLEIRVETFNALAACIVVGVMIIPLVSSLSEDVLSSVPRGLREAGYGLGATKF
                     EVSTRIVLPAGLSGVFASFILAVSRAVGETMAVVLAAGMRPQITLNPLTSIETMTTYI
                     VQIIGGDASYGEPKYLSLFAVGLTLFGVTLILNIISGLVLRHFREVYQ"
     gene            1145..2092
                     /gene="pstA"
                     /locus_tag="VHS97_23585"
     CDS             1145..2092
                     /gene="pstA"
                     /locus_tag="VHS97_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX3451256.1"
                     /translation="MSPSPGPRFRPNGFIRRVIGPIFGAACLLATCTGVAVLFLLLGS
                     ILAAALQGPPDHPWYAIGQNLGELSGLVRKLISHSQSTDPKIAGFKVGIVGSLWLLAL
                     VALFGIPVGVGAGVYLEEYSPPGRLRRIVQTNIANLAGVPSIVYGILGLAVFVRAFGV
                     KALALGPTLLAGVLTLSLLILPIIVITTQEALRAVPQSLRQAALALGGTRWQVVRDHV
                     LPSAMPGIMTGTILGLSRAIGETAPLLMVGAAGSLLQLPKGPLDRYSALPVEIYNYAK
                     EPDTRFQTVAAGGILILVVLLLMMNSTAILIRNRYTRYH"
     gene            complement(2089..2247)
                     /locus_tag="VHS97_23590"
     CDS             complement(2089..2247)
                     /locus_tag="VHS97_23590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451257.1"
                     /translation="MRDDPAAESAANSGGSLEDRSARFSETLINGLLMKEEFSVVSAL
                     FGIMGEPP"
     gene            2246..3031
                     /gene="pstB"
                     /locus_tag="VHS97_23595"
     CDS             2246..3031
                     /gene="pstB"
                     /locus_tag="VHS97_23595"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX3451258.1"
                     /translation="MSGPIVLSTRALSVWYGSSLAIKEITIDIPRNKITALIGPSGCG
                     KSTLLRCFNRMNDLINGARMEGEVRFDGESIAGDDTDPVVLRRRIGMVFQKPNPFPKS
                     IYNNVAWGARINGYQGDMDELVERSLRRAALWDEVKTKLNRTGLDLSGGQQQRLCIAR
                     TLAVEPEVILMDEPCSALDPISTARVEDLMDDLKKDYTIVIVTHNMQQARRVSDMTAC
                     LMLDENSADAHRTGILAEFSPTDILFTNPKDKRTEAYITGRIG"
     gene            3174..>4120
                     /locus_tag="VHS97_23600"
     CDS             3174..>4120
                     /locus_tag="VHS97_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX3451259.1"
                     /translation="MSPYKFVSNPKPTLGVEIELNLVDSHTMALRSGAPQILSEIPPE
                     LASSIKPELLQCYIELNTKVCRNVAEVESDLAEKLRLVRAIADRHELRLFWGGTHPFS
                     PWQDQDVSADERYRGLIQLLQETARRLVTFGLHVHVGVDSGDKAIMICDRILQHLPTL
                     LALSVNSPFWQGRNTGLHSQRSRVMDSLPTAGLPPMMRNWSEYSWLLNNMIETGFITT
                     IREIWWDVRPHHNFGTVEVRICDMPPCLEDVLGITALVQSLVHDLSEEIDRGTYQFDC
                     HPYLIRQNKWRACRYGMDAKLVDPATHQSVEARRVVHNLV"
BASE COUNT          773 a         1280 c         1189 g          878 t
ORIGIN      
        1 agttcctcaa gtattacgtc gagaatctcg agcaacttgc caccaaggcc ctttacgatc
       61 cacccacggc cacggacaaa acagcaaact tcgaaacgct cgccacgctt ttgcccggcg
      121 gcgccggcga agccaagaca accgccaagg tggcggagta aggaacgcac aaattgtccc
      181 ctctctccac atcggccgag aacccggggg gcgcaccggc cgaagccgct ctgtgggcgg
      241 gtcattctca tttcctcgcg ttctgggaaa aagcggtgac ggtcggtctg gtcatttgcg
      301 cgctcatcac ggttttcacc accgccggga tcattgttgt gctcggcacg cagagcctcg
      361 agttttttgc gcgcgcgaag ctcaacctga cggattttct cttcggaacg gtcctcaagc
      421 ctgacgccgc ccccccttcg ttcgggatag tacccttgtt gtggggaacg tttctggtcg
      481 cggcaggctc atcgctgatc gcgctaccga tcgggctctt gagcgcgatt tacctgagcg
      541 agtacgcccc ccggtccgtg cggtcggtgc tcaagccggc tctcgagctc ttggccggag
      601 ttcccacgat cgtctatggc tacctggcgc tgctcctggt cactccgatg ctaaagcgac
      661 tgatggaacc actggaaatt cgtgtcgaaa cattcaacgc gctagcggca tgtatcgtgg
      721 tgggagtgat gatcatcccg cttgtttctt ccttgagcga agatgtgctt tcatcggttc
      781 cccgcgggct ccgagaggcc ggctacgggc tgggagcgac caagttcgag gtctcgactc
      841 gcatcgtact tcccgccggt ctctccggcg tcttcgcttc gttcatcctg gctgttagcc
      901 gggcggtcgg cgagacgatg gcggtggtgc tcgctgccgg catgcgtcct cagatcacgc
      961 tcaaccccct gacctcgatc gagacgatga ccacctacat cgttcagatc atcggcggcg
     1021 acgcctcgta cggcgaaccg aaatacctgt cgctgttcgc cgtcggcctg acgctctttg
     1081 gcgtgacctt gatcctcaat attatctcgg gattggtact gcgccatttt cgcgaggtct
     1141 atcaatgagt ccgtctccag gacctcgctt ccgtcccaat ggtttcataa ggcgggttat
     1201 cgggccgatc ttcggcgcgg cctgcctgct cgcaacctgc accggcgtgg cggtcctctt
     1261 tctgctctta ggctcgattt tggcggccgc actgcaaggg ccgcccgacc acccttggta
     1321 tgcgatcggg cagaacctcg gcgaattgag cggattggtg cgaaagctga tttcacattc
     1381 acagtcgacc gacccgaaga tcgcggggtt caaggtcggc attgtcggga gtctttggct
     1441 gctggcactg gtcgccctgt tcggtattcc ggtcggagtg ggtgccggtg tctatctcga
     1501 ggagtattcc ccgcccggcc gtctccgtcg gatcgtacag accaacatcg ccaatctcgc
     1561 cggtgtgccg tcgatcgtct atgggatcct cggtctggcc gtgttcgtgc gggccttcgg
     1621 ggtcaaggcc ctcgcgcttg gcccgactct cttggccgga gtgctcaccc tcagcctgtt
     1681 gatcctgccg attatcgtga tcacaaccca agaggcattg cgcgccgtcc ctcaatcgct
     1741 ccgtcaggcg gcactggcac tgggagggac gcgatggcag gttgtccgcg accacgtgct
     1801 tccctcggcg atgccaggaa tcatgacggg cacgatcctg gggctctcac gcgccattgg
     1861 tgagacggct cccttactca tggtcggggc agccggctcg ttactccagt tgcctaaagg
     1921 tccgctggat cgctattcgg ccctcccggt ggagatctac aattatgcca aggagcccga
     1981 tacccggttt cagaccgtcg ccgccggcgg catcctgatc ctggtggtgc tcttgctcat
     2041 gatgaattcc accgccattc tgattcggaa ccgttacacg cgttatcatt agggcggctc
     2101 acccatgatc ccgaaaagcg cggacacgac tgagaattct tccttcatca agagaccatt
     2161 gatgagcgtc tccgaaaaac gcgccgaccg atcctcgagc gaaccacccg aattggcggc
     2221 tgattccgcc gccggatcat ctcgcatgag cggcccgatc gttctgtcca cgcgcgctct
     2281 ctctgtgtgg tacgggtcga gcctggcgat caaggagatc acgatcgata tcccgcgcaa
     2341 caagatcacg gccttgatcg gaccgtcggg ttgcggcaaa agtaccctgt tacgttgttt
     2401 caatcggatg aacgacctca tcaacggtgc acggatggag ggcgaggtcc gctttgacgg
     2461 cgagtcgatc gcgggcgacg acaccgaccc cgtcgttctg cggcggcgga tcggcatggt
     2521 ctttcaaaag cccaacccgt ttcccaagag tatttacaac aacgtcgcct ggggcgcgcg
     2581 catcaatgga tatcaggggg acatggacga gctggtcgag cgctcgctgc gtcgcgcggc
     2641 cttgtgggat gaggtgaaga ccaagttgaa tcgcaccggc ctcgaccttt ctggcggaca
     2701 gcaacaacgc ctttgcatcg cgcggacact ggccgtcgag cccgaagtta tcttgatgga
     2761 cgaaccttgc tcggcccttg accccatctc gacggcccgc gtcgaagacc tgatggatga
     2821 cctcaagaaa gactatacga tcgttatcgt cacccataat atgcagcaag cccggcgcgt
     2881 cagcgacatg acggcttgcc tgatgctcga cgagaattcg gctgacgcgc atcgcaccgg
     2941 tattctcgcc gagttcagcc cgacggatat cttgtttacc aatcccaagg acaagcgcac
     3001 cgaagcttat atcaccgggc gcatcggttg aatcccagcg agaaatccag tcaacataga
     3061 actcgagcga ataggcgtcg tcgcgatcga agcgtcgatc gtggactggc ggcatccaaa
     3121 gtcctcgctc gaagcgtgat cctgctggcc aatctctccc ggagcgacac cgcgtgagcc
     3181 catacaagtt cgtcagcaat cccaagccca cactcggagt cgagatcgag ctcaatctgg
     3241 tcgatagcca tacgatggca ctgcggagcg gcgctcccca gattttgagc gaaatcccgc
     3301 cggagcttgc gagctcgatc aaaccagagt tgctccagtg ttacatcgag ctcaacacca
     3361 aggtttgccg caacgtggcc gaagtcgaga gcgacctggc cgaaaagctc cgtttggtcc
     3421 gcgcgattgc ggaccgtcac gagctgcgac ttttctgggg aggaacccat ccgttttcgc
     3481 cctggcagga tcaggatgtc tcggcggacg agcggtaccg cggcttgatc cagctccttc
     3541 aggaaacggc ccgacggctg gtgacctttg gcctgcatgt ccacgtcggc gtcgattcgg
     3601 gcgacaaggc gatcatgatc tgcgatcgga tcctccagca cttgcccaca ctgctcgcgc
     3661 tttctgtgaa cagcccgttc tggcagggcc gcaacacagg gctgcactca cagcgcagca
     3721 gggttatgga ctcgctgccg acggctgggc tgcccccgat gatgaggaac tggtccgagt
     3781 atagctggct gttgaacaac atgatcgaga cgggtttcat cacgacgatc cgcgaaattt
     3841 ggtgggatgt tcgcccgcat cacaacttcg gaacggtcga ggtccggatt tgcgacatgc
     3901 cgccctgcct ggaagacgtc ctcggcatca ctgccctcgt tcaatcgttg gtacacgacc
     3961 tctccgaaga gatcgaccgc ggcacctatc agttcgattg ccatccgtac ttgatccggc
     4021 aaaacaagtg gcgagcttgc cggtatggta tggacgccaa gctcgttgat cccgctacgc
     4081 atcagtccgt ggaagcacgc cgagtcgtcc ataatcttgt
//
LOCUS       DATAQL010001369         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4108073, whole genome shotgun sequence.
ACCESSION   DATAQL010001369 DATAQL010000000
VERSION     DATAQL010001369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4108073"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..>2042
                     /locus_tag="VHS97_23605"
     CDS             34..>2042
                     /locus_tag="VHS97_23605"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3451260.1"
                     /translation="MSTGDGASPTPPDPMMTGQDCSDFLSSILRIDVDHTEPGKAYRI
                     PADNPFINTPGVRPEIWAYGFRNPWKMSIDKVTGKLWVGDVGWELWELVFDVKRGGNY
                     GWSVMEGPQPVNVEARRGPTPILPPIKVHPHSEATSITGGYVYRGTRLPELAGTYIYG
                     DFMTGIIWGLRSLGDKITWQRELARTPVHLVAFGESTSGELYMLDHDRTHQIYRLVRS
                     PTLTDVSDFPRRLSQTGLFTSTIHQQPAPGVIPYSVNAELWSDGATAERFVAVPEDGR
                     VGLENRGSWQFPDGSVLVRSISIEREQGQPASRRRMETQILHLEADAWRPYSYVWNDD
                     QTDAFLAEPAGSTVNVLQKTPGGRREVYYRVYARTECVLCHSPWVEKKTTIYGIQSAS
                     PLGVNTPQLNRPSGNDANAPNQLSAWHKLGLLAWTPEVGKLPRLVDPYDESADLDRRA
                     RSYLQTNCAHCHQFGSGGSANMELRFDLPLDKTKTIGQRPIQGTFNISGARIIAPSDP
                     AGSVLYYRIAKLGGGRMPRVGSQQVDERAIRMIHDWIARMPADGPSNGAPSPTKIAPE
                     DRAAIETLRQGDRLSPSDRTTAISRLALSTRGALMLLAFIDQGSCSESFKRDVVSITK
                     NSPLVEVRDLFERFIPEQERTKRLGDVINRAAILALPGDSARGLSV"
BASE COUNT          421 a          626 c          599 g          396 t
ORIGIN      
        1 tgtctcgtct tcggcaatga cggatgtctc tatatctcca ctggagacgg tgcgtcaccg
       61 acccctccgg acccgatgat gaccggccaa gactgctccg actttctgtc gagtatcttg
      121 cgcatcgacg tggaccatac cgaaccgggc aaagcgtacc gcattccggc cgacaatccg
      181 tttatcaata cgccgggagt tcggcccgaa atttgggcct atggctttcg aaacccctgg
      241 aagatgagca tcgacaaagt taccggcaaa ctgtgggtcg gtgacgtggg ctgggaactg
      301 tgggaattgg tctttgacgt caagcgcggg ggaaattacg gctggagcgt gatggaagga
      361 ccgcaacccg tgaatgtcga agcacggcgc ggacccacgc ccattctgcc tccaatcaag
      421 gtgcatcccc actctgaagc cacctcaatc accggtggct acgtctatcg cggcacgcgc
      481 ctgccggaac tggccggcac gtatatctat ggtgatttca tgactggaat catctggggc
      541 ctgcggtctc taggggacaa gatcacgtgg cagcgcgagc tggcccgaac ccctgtgcat
      601 ctcgtggcgt ttggtgagag cacaagcggt gagctctaca tgctcgacca tgatcggacg
      661 caccagatct atcgcctggt acgcagtccg acattgacag acgtgtccga tttcccgcgc
      721 cgattgagcc agaccggcct tttcacctcg accatccacc agcagccggc gccgggcgtg
      781 atcccctact cggtcaacgc cgaactctgg tccgacggag cgaccgccga aaggtttgtg
      841 gccgtccctg aggatgggcg tgtcggtctt gaaaaccgag gaagctggca gtttcctgat
      901 ggctcggtcc tcgtccgatc catttcgatt gaacgagagc agggccagcc ggccagtcgg
      961 cggcgaatgg aaacccagat ccttcacctc gaggcggacg cgtggaggcc atattcatac
     1021 gtatggaacg acgaccagac cgatgccttt ttggctgaac ccgccgggtc gaccgtgaac
     1081 gtgcttcaga aaacaccggg cgggaggcga gaggtctatt accgcgttta tgctcgaacc
     1141 gagtgcgtgc tctgccacag tccctgggtt gaaaagaaga ccacaatcta cggcattcag
     1201 tccgcttcac cactcggagt caacacgccc cagctgaacc gcccctcggg gaacgatgcc
     1261 aacgcgccca accagctgag tgcatggcac aagctgggat tgctcgcctg gactcccgag
     1321 gtcggcaaac tacctaggct ggtcgatcct tatgacgagt cggccgatct cgatcgtcga
     1381 gctcgatcct atctccaaac gaactgcgcc cattgccatc aattcggctc cggcgggtca
     1441 gctaatatgg aactgagatt cgacttgccg ttagataaga ccaaaacgat tggtcagcgg
     1501 ccgatccagg ggacattcaa tatctcggga gccaggatca tcgctcccag cgacccggcc
     1561 gggtctgtgc tgtattatcg aatcgccaag ctgggaggcg gccgcatgcc gcgcgtcgga
     1621 tcgcagcagg ttgacgagcg cgcgatccgc atgatccacg attggatcgc tcggatgccc
     1681 gcggacgggc cttctaacgg ggcccccagt ccgaccaaaa tcgcccccga agatcgggcg
     1741 gcgattgaaa cgttgcgtca aggcgatcgg ctttcgccct ctgaccgaac gaccgccatc
     1801 agccgcctcg cattatcgac gcgcggggcc ctcatgctcc tggcattcat cgatcagggc
     1861 tcctgctccg agagcttcaa gcgcgatgtg gtgtcgatca cgaagaatag tccgctcgtc
     1921 gaagtgcgcg atctgttcga acgattcatt cccgagcaag agcgaacgaa gcggctgggt
     1981 gacgtgatca accgggcagc aattctggcg ttgcctggcg actcagcccg cgggctctcc
     2041 gt
//
LOCUS       DATAQL010001370         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2124712, whole genome shotgun sequence.
ACCESSION   DATAQL010001370 DATAQL010000000
VERSION     DATAQL010001370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124712"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2039
                     /locus_tag="VHS97_23610"
     CDS             <1..2039
                     /locus_tag="VHS97_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX3451261.1"
                     /translation="DEPQPSIDELGRIVAWIEKDFLAAQCANGESSAPVVLRRLNRRE
                     YNNTIRDLVGLDLHLADAFPADDIGFGFDNVGSALNISPVHVEKYLDAAEIALQKAIV
                     LPDAAGFSPAELIGLKTYPLPPTEAVEFKHSLKPGRYLADFSLVRVGIPESVAPPKLV
                     IGFGKDSRTVDAVRVQDETVVYRYWLTVAQGDNLVHVKLAPGQEKGPNVVRPKELADN
                     VSGDKRYGNEIGLHVDSMVVRGPVVVRADQLPESHRKILFRTPLYGDQSRLDCAREVI
                     ARFTERAFRRPAQPEEVERVLQIYRLAHDRGESFERAVQLALTTVLASPRFLFLVEPE
                     EAREDRPLNDFELASRLSYFLWSTMPDDELFREAREKTLRFNLRHQVVRMLDDPRSEE
                     FVANFTGQWLQLRKLAGVARDKDLFRGFDDTLRDAMRQETERFFAFILRNNRSALELI
                     DADYTFVNETLARHYEMEGVKGSEFRRVSLKDRRRGGVLTQASILTLTSNPNRTSPVK
                     RGQWILQQILGTPPPPPPPEVAKLDESPQAAEVASLRERTELHRSNPQCASCHQQMDP
                     LGFALENYDAIGRFRTTDGEFPIDPSGELIGGRKFADAKELKQLLGSTAATKFARCLV
                     ENMLTYGLGRGLEAYDFCTVDDIRQLLAANDYRIRSVIFGIVESKAFQNRGVAR"
     gene            2128..>2578
                     /locus_tag="VHS97_23615"
     CDS             2128..>2578
                     /locus_tag="VHS97_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX3451262.1"
                     /translation="MTMNVTRRTLLRGMGAALALPWLESLTPIARAAAKSGTPPVRMC
                     YWYVPNGVHLPTWFPKQEGTLIELPETLEPLSFARDYLNCFHGLTHNTAKTNGDSEGC
                     GHGQGSASFLTGAQAYKTQDAVRVDISADQLYARHVGNQTRFPTLELG"
BASE COUNT          509 a          802 c          768 g          499 t
ORIGIN      
        1 cggacgagcc tcagccatca atcgacgagc tcggcagaat tgttgcatgg atcgagaaag
       61 acttcctggc cgctcagtgt gcgaatggcg agagctcggc accggtcgtc ttgcgccggc
      121 tcaaccggcg cgagtataac aacacgattc gcgacttggt cggcctggat ctccacctcg
      181 cggatgcctt ccccgcggac gacatcggtt tcggcttcga caacgtcggc tccgcgctca
      241 atatctcacc cgttcatgtc gagaagtatc tcgacgccgc ggaaatcgcc ttgcaaaagg
      301 cgatcgtttt gcccgacgcc gccggctttt cgcccgccga attgattggc ctgaaaacct
      361 atccgcttcc tcccaccgaa gccgtcgaat tcaaacattc gctcaagccc ggacggtacc
      421 tggccgactt cagcctggtg cgggtgggca ttcccgagtc cgtcgcgccg cccaagctgg
      481 tgattggctt tggcaaggac agtcggacgg tggatgccgt gcgcgtgcag gacgagacgg
      541 tcgtataccg ctactggctc acggtcgccc aaggggacaa tctggtccac gtcaagctgg
      601 cgcccggtca ggaaaagggc ccgaatgtcg ttcgacctaa ggaactggcg gacaacgtca
      661 gtggcgataa acgctacggg aacgaaatcg gtttacacgt cgactcgatg gtcgtgcgcg
      721 ggcccgtcgt cgtacgcgcc gaccagctcc ccgagtcgca tcgaaaaatc ctgttccgca
      781 cacccctcta cggcgatcaa tcgcggctgg attgtgcccg cgaggtgatt gcgcggttca
      841 ccgagcgcgc attcaggcgg ccggcccaac cggaggaagt cgaacgagtt ctccagatct
      901 atcgcctggc gcacgatcgg ggcgaaagct ttgaacgcgc cgtccagctt gccctcacaa
      961 cggtgcttgc ctcgccccgg tttttgtttc tggtcgaacc cgaagaagca cgcgaggatc
     1021 gcccgcttaa cgacttcgag cttgccagcc ggctctcgta ttttctctgg agcaccatgc
     1081 ccgacgatga gctgttccgc gaggcgcgcg agaaaacgct gcgattcaat ctccgtcatc
     1141 aggtcgtccg gatgctcgat gaccccaggt ccgaagagtt cgtggccaac ttcaccggcc
     1201 agtggctgca gctccggaaa ctcgctggag tggctcgcga caaggatctc ttccgcggtt
     1261 ttgacgacac cttacgtgat gcaatgcggc aagagaccga gcggttcttt gccttcatcc
     1321 tccgcaacaa tcgcagcgcg ctcgagctga tcgatgccga ctacacgttt gtcaacgaaa
     1381 cactggcgcg ccattacgag atggaaggtg tcaagggatc ggaatttcgg cgcgtatctt
     1441 tgaaggatcg gcggcggggt ggtgtcttga cgcaagcgag catcctcacg ctgacttcaa
     1501 atccgaatcg gacgtctccc gtcaagcgcg gtcagtggat cttgcagcaa attctgggga
     1561 cgcctcctcc gcctcccccg ccggaagtgg ccaagcttga tgaaagccct caggccgccg
     1621 aagtcgcctc gctgcgcgag cggacggagc tgcaccggtc caacccacaa tgcgcatcgt
     1681 gccatcagca gatggatccc ctgggctttg ccctggagaa ttacgacgcg atagggcggt
     1741 tccgaaccac ggatggcgaa ttcccgatcg atccgtcggg cgagctgatc gggggacgca
     1801 agtttgccga cgccaaagaa ctgaagcagc tgttggggtc gacggccgcg acgaaatttg
     1861 cgcggtgttt ggtcgaaaac atgctaacct atggcctggg acgaggtctg gaagcttacg
     1921 atttctgtac ggtcgatgac attcgccagt tgctcgccgc caacgactat cgcattcgca
     1981 gcgttatctt cgggatcgtg gaaagcaagg cgttccagaa tcggggcgta gccaggtagg
     2041 tgaccgtcgt tcggctagtc ggcccgcaac ctggggccgg ccctgagaaa tcgcccgccg
     2101 ttctagtgtg atcaggggaa aaaagcgatg acgatgaacg tgacccgccg aacgttgctc
     2161 cgtggcatgg gagccgccct ggcgttgccc tggctcgaga gtttgactcc cattgcacgc
     2221 gcggccgcga agtcgggcac gccgccggtc cggatgtgct actggtacgt tcccaacggc
     2281 gtgcacctgc ctacctggtt tccgaagcaa gaaggaacgc tcatcgagtt gcccgagaca
     2341 ctagagcccc tatcgttcgc ccgcgactat ctcaactgct ttcacggctt gactcacaac
     2401 acagccaaaa ccaacggcga cagcgaaggt tgcggccacg gtcagggttc ggcgagcttt
     2461 ttaaccggtg cccaggccta caagacgcaa gacgccgttc gcgttgatat ctccgccgac
     2521 cagctctacg cgcgccacgt cggcaaccag acgcggtttc ccaccttgga gctcggct
//
LOCUS       DATAQL010001371         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_444244, whole genome shotgun sequence.
ACCESSION   DATAQL010001371 DATAQL010000000
VERSION     DATAQL010001371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444244"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VHS97_23620"
     CDS             <1..626
                     /locus_tag="VHS97_23620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3451263.1"
                     /translation="ASSPGRGHGSTFRLELVTVLAPVSAKPPRAGGRPSVSLARTPDR
                     RSLRILLVEDNRDTLRYLATVLRQRGHQVVTADRLSTALTAVKEAETPFDLLLSDIEL
                     PDGDGLNLMRDINAHGRMAGVAMSGFGSEEDLRQSREAGFFDHLTKPIDLNRLDEAIR
                     GATESEASRTANTEDASRPFRLRGEANSSGEFKIVWAREPESENSSL"
     gene            complement(645..>1652)
                     /locus_tag="VHS97_23625"
     CDS             complement(645..>1652)
                     /locus_tag="VHS97_23625"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX3451264.1"
                     /translation="VLSPVTGYLASFVATLSQRWNAFWYTPADPTLLGMIRILTGLML
                     VYTHAVWGLALHDFFGPDGWISRDLVELVQTNQFAYSLWWLVPPSWLWPAYAAAMVIL
                     VLFTVGLWTRITSLLSLLIVISFVNRVPEALFGLDKLNVILTFYLAIGPSGSALSLDR
                     WLALERGASLPRAPRQSVSAAFTLRLIQVHMCIVYFFAGISKLQGVAWWNGQAMWLAF
                     GNLEYQSADMTWLAWHPWAVNFLTHFTALWELSFCVLVWIPLLRPLVLIAAVILHVAI
                     GACLGLWTFSLIMLVGCASFLPRDGVARLVTALTSRPKQRVRTKGSGRFSEEKGARPS
                     N"
BASE COUNT          378 a          525 c          487 g          262 t
ORIGIN      
        1 cggcttcgag tccaggacgt gggcacggct cgacattccg gttagaactg gtgaccgttc
       61 tcgccccggt cagcgccaaa ccaccccgcg ccggcggacg gccttccgtc tcactggccc
      121 gaactcccga ccggcgcagc cttcgtatct tgctcgttga agacaatcga gacacgctcc
      181 gctatctggc gaccgtcctc cgccagcgag gtcaccaggt ggtcacggcc gatcgactct
      241 cgaccgcgct caccgcggtc aaggaggccg agaccccctt cgacttgctg ctgtcggaca
      301 tcgagctacc cgacggcgac gggctcaacc tgatgcgcga tatcaacgcg cacggaagaa
      361 tggccggcgt tgccatgagt ggctttggca gtgaggaaga tctgcggcag agccgcgagg
      421 ccggattttt cgaccatctg accaagccga tcgacctcaa ccggctcgac gaggcgattc
      481 ggggtgccac cgaaagcgag gcctcgcgca ccgccaatac ggaagatgcc tctcgaccct
      541 ttcgcctacg aggcgaggcc aatagctccg gggaattcaa aatcgtctgg gcgcgcgagc
      601 ccgaatccga gaattcaagt ctctgacaac agggccagga cccttcagtt agagggcctg
      661 gccccttttt cttcagaaaa gcgtcccgac ccttttgttc tgaccctttg cttgggccgg
      721 gaggtgagcg cggttaccaa cctcgcgact ccgtctctcg gcaagaacga cgcgcagccc
      781 accaacatga tcagcgaaaa cgtccacagt cccaggcagg ctccaatcgc cacgtgcaag
      841 atgaccgcgg cgatcaagac cagcggccgc aacaggggaa tccagaccaa gacgcaaaac
      901 gaaagctccc agagggccgt gaagtgggtc aggaagttca ccgcccacgg atgccaggcc
      961 agccacgtca tgtcggccga ctggtactcg aggttgccga aggccagcca catcgcctgc
     1021 ccattccacc atgccacccc ctgcagtttt gagatgcccg caaaaaaata cactatgcac
     1081 atgtgaactt gaattagacg tagagtgaag gctgcggaca cgctttggcg gggtgctcgg
     1141 ggtaggctgg cgccccgctc tagggccagc cagcgatcga gcgaaagcgc gctgccgctg
     1201 gggccgatgg cgaggtagaa cgtgaggatg acgttcagct tatcaaggcc gaacaaggct
     1261 tccggaacgc gattcacaaa cgaaatcacg atcaggagcg acaagagtga cgtgatgcgc
     1321 gtccacaaac cgacggtgaa aagtaccaga atcaccattg cggcggcgta ggccggccag
     1381 agccacgagg gcggcacaag ccaccagagc gagtaggcga attgattcgt ctggaccagc
     1441 tcaaccagat cccggctaat ccagccgtcg ggcccgaaaa aatcgtggag cgccagcccc
     1501 cagacggcgt gcgtgtaaac cagcatcagg ccggtcaaaa ttcggatcat tcccaagaga
     1561 gtcgggtcgg ccggcgtgta ccaaaacgcg ttccatcgct gactcaaggt ggccacgaag
     1621 cttgcaagat acccggtaac cgggctcaaa ac
//
LOCUS       DATAQL010001372         3673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2887915, whole genome shotgun sequence.
ACCESSION   DATAQL010001372 DATAQL010000000
VERSION     DATAQL010001372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887915"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..2350
                     /locus_tag="VHS97_23630"
     CDS             260..2350
                     /locus_tag="VHS97_23630"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3451265.1"
                     /translation="MIHGLLIGTLALMLGTTVQPDAGQPEDPYQWLEDVTGEKPLAWV
                     KERNAESTGELTGSEQFQKLNHRILEILDSDARIPAIQKIGPYYYNFWRDAQNPRGLW
                     RRTTLDEYRKLKPDWEIVLDLDALGKEEKVNWVWHGAQTLKPEYKLALISLSRGGADA
                     SVVREFDLTKKAFITDGYTLPEAKSQVSWRDRDSVFVGTDFGAGSMTKSGYPRIVKEW
                     KRGTPLAEAALVLEVQPDDMMVTAFRDLTPGFERDVVVRRPTFWTNETFLRRDGRLNK
                     IDKPDDARVTLVREWLLLQLRSDWTTGGKTYAAGALLATDLEKFLKGDRVFDVLFEPG
                     ERKSLVGFNPTRHHILLTELDNVRSRIYVLTHRDQGWHREPLPGVPDFGEVNVDDVDA
                     DESDDYFQNATDFLTPTTLAFGTVGAGPATTLKQLPAFFDAGGLAVSQHEAVSNDGTS
                     IPYFEVARKNMTNDGKNPTLLYGYGGFEIPMLPSYRPAVGAAWLETGGVYVVANIRGG
                     GEFGPQWHQSALKANRHRAYDDFIAVAEDLIKRKVTTPQHLGIQGGSNGGLLMGNMLV
                     RRPDLFGAIVCQVPLLDMRRYHHLLAGASWMGEYGNPDLPEEWAFIRGFSPYHNVEKS
                     MKYPRTLFTTSTRDDRVHPGHARKMVAKMKQLGHNVIYYENIEGGHAGAADNKQAAFM
                     QALAYSFLWNELKN"
BASE COUNT          803 a         1055 c         1084 g          731 t
ORIGIN      
        1 catcgtacgg ggagacccca ctttgatcca gacatcgata tcgtggcaac gagatccgtc
       61 gaaaatgatg tatgaacgta gattgatcat gagcttgccg acctttgcgg atcacaaacc
      121 tgtttctggc gctcgcctcg aacgcccagg agaccttgaa aaggtatgta atcaaccacc
      181 gaatcgacta ataatggttc tgaggtaaga atggggaaga cttccaacga cgtccaacac
      241 tgagccagag gaaacctcga tgattcatgg tctattgatc gggacactcg cactgatgct
      301 aggcacaacc gttcaacctg acgccggaca acccgaagac ccttatcagt ggctggaaga
      361 cgtcaccggc gaaaagcccc tggcgtgggt caaggagcgc aacgccgaga gcaccggtga
      421 gctgaccggg tctgagcaat ttcaaaagct caaccaccga atcctggaga tcctcgattc
      481 cgacgcccgc atcccggcca tccagaagat tggcccgtat tactacaatt tctggcgcga
      541 cgcccagaac ccgcgcggac tctggcggcg aacaacattg gacgagtacc gcaaactcaa
      601 gcccgactgg gaaatcgtgc tggatctcga cgctttggga aaagaagaga aagtcaactg
      661 ggtctggcac ggtgctcaga ccttgaaacc cgagtacaaa ctcgccctca tatccctttc
      721 acgcgggggc gccgacgcca gcgtggtgcg cgaattcgac ctgacaaaga aggcgttcat
      781 cacggatggt tacaccctgc ccgaggccaa gagccaagtt tcgtggcgcg accgcgatag
      841 cgtattcgtc gggactgact ttggagcggg atctatgacc aagtcgggtt atccgcgcat
      901 cgtcaaggaa tggaaacggg gaacgccgct ggccgaggcg gcattggtcc tcgaggtcca
      961 gcccgacgac atgatggtca ccgcttttcg agacctcacg ccgggctttg agcgcgacgt
     1021 cgtggtgcgc cgaccgacat tctggacgaa tgaaacgttt ctccgccgtg acggcaggct
     1081 caacaagatc gacaagcccg acgacgcccg agtcacattg gtccgcgagt ggctcttgct
     1141 ccagcttcgg tccgactgga cgacgggtgg taagacttat gcggccggtg cattacttgc
     1201 gaccgatctc gagaaattcc tgaaagggga tcgcgtcttc gacgtgctct tcgagcctgg
     1261 cgaacggaag tcactcgtag gtttcaatcc gacgcggcac cacattctcc tgaccgaact
     1321 cgacaacgta cggagccgga tctacgtctt gacgcaccgc gatcaaggtt ggcatcgtga
     1381 gccgttgccg ggcgttcctg actttggcga ggtgaatgtc gacgatgtcg acgccgatga
     1441 atccgacgat tactttcaga acgcgaccga ctttctgacg ccgaccacgc tggccttcgg
     1501 tacggtcggc gcgggtccgg cgacgacgct caaacagctt cccgcgtttt tcgacgccgg
     1561 tggcctggcg gtttcgcagc acgaggctgt ctcgaacgac ggcacgagca ttccttactt
     1621 cgaggttgcc cgcaagaaca tgactaatga cggcaagaac ccgaccttgc tctacggtta
     1681 cggcggcttt gaaataccga tgctgccctc gtaccgcccc gcggtgggcg cggcttggct
     1741 cgagacagga ggggtctatg tggttgccaa catccgcggc ggcggcgaat ttggaccgca
     1801 gtggcaccaa tcagcactca aggccaaccg gcacagggcc tatgatgatt tcatcgcggt
     1861 ggccgaagac ctgatcaagc gcaaggtgac cacgccgcag cacctgggca tccagggagg
     1921 gagtaacggc ggtctcttga tgggcaacat gctggtgcgt cgccccgacc tttttggcgc
     1981 gatcgtctgt caggtcccgc tcctggatat gcggcggtat caccatctgc tcgccggcgc
     2041 gagctggatg ggggagtatg gcaatcccga tttgcccgag gaatgggcgt ttatccgcgg
     2101 cttctctccc tatcacaatg tcgaaaaatc gatgaagtat ccgcggaccc ttttcacgac
     2161 ctcgacccgt gacgaccgtg tccatccggg gcacgcgcgg aagatggtgg ccaagatgaa
     2221 gcaactcggt cacaacgtca tctattacga aaacattgag ggaggccacg ccggtgccgc
     2281 cgacaacaag caagcggcgt tcatgcaggc gctggcgtat tcattcctgt ggaatgagct
     2341 gaagaactga tgggacgacg aactgtgatt gtcgtcccat cagtggccga tcggatcgaa
     2401 cgcttcgagc agcagcgccg cgattggagg cgggacaggc tcgtgtttgc tcttttggtc
     2461 ggaactccga tattgttgga cgatgcgttt gacgcaatgg gagggcggcc gtcatgcgac
     2521 gaaaatgcaa ccggcgcccg tttgcaactc atgtctcccg tatcgtggga tgcggtagaa
     2581 atgcttctcg ggacgcgagt gcaattcccg ccgcctccac atcttatgtc ataactcgtt
     2641 agcttataaa gccttatgtc tacaagggac gcgagtaagt gacccgccgc cttgagaatc
     2701 tcaagctgcc agtgttgatc ggaagcatcg cactaagaac aacttgagtc gttgttttca
     2761 gaacgacgct gagtcgattc gcgttccaat ttgcaggtat cgccgccgaa ctcccggcca
     2821 aaatccgacg acggccgatt tttgatgccg agaagccgaa gattgcttgg tgtcgtccga
     2881 ctagcccggc gagagtttag ttcgacaagg acctatcttc cgccagcccg gagtcgtact
     2941 gctgaggagc ctacattccc ggacgcgcat cggtaagccg gcccgagttc ggtatggtgt
     3001 ccttgcgtca ggaaagcttg ggaaaggagg aagagactcg aactcagaaa cctttcgttg
     3061 gaaacctgcg ggtacgaatc ccgcctggca aagccgggcc agcagccaga agcgagtctt
     3121 gcgtggcggt ggggcgaccc accctgcgaa gcgtagacag cgaggtcaaa ggtcgtgtga
     3181 ttgagccccg aaattacaag ccgtggggag ccttcgtcgt ggacacagac gggggccgcg
     3241 tcaggcgcgt cgcacttggt cccacagggc gcgtctggtc ctaccggggt cgtagagcac
     3301 ggacaatgac catcagggtt ctccaggaac ctgagagccc ccgacgtttc catcgcaata
     3361 ccggatgggg agaccggtac cgacaactcc ctggcccccg gtgggacgcc cccggccgag
     3421 gggtgggacc gatcactaat ccggcagcga tttcaatcgt ttggagggca acgctcggag
     3481 ttcttgttgc cagcacgtcg atgagaccgt ttatctctct gtcttgttcc cgggaacacg
     3541 atcggatgag atcagaccga tcgcgaactc agacccaggt tccatctttt ggagagcgtg
     3601 accaaacagc ggctccttcc ttggtccaac cggatgcgca aaaccgtctt cttcattgtc
     3661 ttgttccaac gat
//
LOCUS       DATAQL010001373         7094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1210135, whole genome shotgun sequence.
ACCESSION   DATAQL010001373 DATAQL010000000
VERSION     DATAQL010001373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210135"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VHS97_23635"
     CDS             <1..816
                     /locus_tag="VHS97_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX3451266.1"
                     /translation="ILVQRGRTVCLIQLSQAVINSADLLVVGFLSVASDVGRYGAPHR
                     MVTAFLTFGLILQQAAFPTLSRLWRQTAGAGQEALDSLVEVLMTGLVPVAVGCTVLAD
                     PLVHLFLPNGYAGAGFLLALGIWRAPLLILAYLYQITLIALNRETVGVRTLVAGALVI
                     VPLVALLRLSFGLPGAAAGVLLVGLALVLAGYSCLAREGRQPAWHHHLARPILASLAM
                     VPACMALKHYHVLLAVVGGAVTYSAVWLALGGLRHTRLWGRALRPLARSGTTS"
     gene            907..2274
                     /locus_tag="VHS97_23640"
     CDS             907..2274
                     /locus_tag="VHS97_23640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3451267.1"
                     /translation="MKFRMITTFFGEHSFGGDAAYVDRLSQALCRRGHEVHVYYCVDA
                     FNTVRGDHPLRPYTPPPGIHLHPLKSRFGILSPLATQTTGLPLLKTAELREALDDPET
                     DVVHFHNISLVGGPGVLGLGANRRAVRIMTAHEHWLVCPMHLLWKYDRKPCDAPTCVS
                     CCLQGRRPPQVWRATRAIERGLRNLDALVFPSRHALEEHRRRGLAAHVPLVHVPYFLP
                     DDWSAGVEQAPRTVPDRPYVAAAGRLVKMKGFQHLIPLMRFLPEVDLHIAGTGPYEPA
                     LRAMAAELPNVRFEGLLGGQALAALFRHARAVVVPSLFPETFGYVVLEAFAVGTPVIV
                     HEGGGALYETGFLSGGGLAYRSDTELLTAMRRMVHDRALHDELAARGVAMRMGPWSET
                     EHVDEYLRLIDQSQSARQRKPFPRPHSLNRPNAAPARVDSLPWSELGNEPMKAAGINR
                     PHGMI"
     gene            2287..4794
                     /locus_tag="VHS97_23645"
     CDS             2287..4794
                     /locus_tag="VHS97_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451268.1"
                     /translation="MRERRWRPTALVSALTLVILAGVCFARLVVQPAAIIVDEERPSL
                     DHANPGEPRGIGNDAAFLFLPHHLSIAKVIRTFGHVPSWDVRGFGGRPLIGNPQGGTF
                     YPPVWLVWWSGAPAALGWLTVGHLLWGGAGMYVLLRSLGAGPWGATIAAASYQASPLL
                     LAHTFEGHYPHVWAACWFPWAFWAFHDHRSGHVRGRLLLPIILVFTYLTGHPQEWLLL
                     VLALTAWCLGAAWRLWRAQGPRESVAQVVWWAGTLALSIGIAAVEIAPELAVRPWLTR
                     NSDAPAGIEIPRRYHLGAGNLLQLLSPTALGGPSDYFGDDNYWETLFSIGLVPLVLAV
                     TAAVWHRDRKQIRGWLWLMGLAIWFACGRRLLFFVIAYLTVPGVSWFRVPARALFLAN
                     LAGAALAGLGVETLQIHRGRSQFWHKYAVRFASVFVIGLACLFSIRSYHGSDSSSRIA
                     LAAGRVLDDASFRSVLATIAAVILAGCLVRGWRNPLLAGRLLGLLVLCELGWNGVSLI
                     QVARADPFLGHHPVSAALTQFRHDSPGAGRLRVKARDLFYSDLQAASIDVEKTNINDV
                     FQLAHASRLYAQLYPVASRLRRSIDDHMHEAVEYHRRQIRQAVFDRFSVAYLVSDRYE
                     SDPGWPVAAQGEWHGSPWVIQQNTSALPRAYVVPTAAIVSEDESSPRARFLDVDPRES
                     VLMNDDPLVHLADRPRQPFTPAQWTSLDPDHPVITVTTHGAGLLVVSDTWMPGWTARV
                     DGAKTPIYRGNIAQRVIPLWQAGRHTIEMDYVPPGLALGCILSALSLLSWALMCGSAI
                     AKSLKNEVPRHCGDFRVLTTRTTRRSPASSSHLRIPG"
     gene            complement(4826..5647)
                     /locus_tag="VHS97_23650"
     CDS             complement(4826..5647)
                     /locus_tag="VHS97_23650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451269.1"
                     /translation="MRVATILMLGTLSLGTGCTNSALRNSTVSQANTMTELQYQMVLE
                     NLATLAANAGTMPWHANITGGTTQMTDSAQGTLGIGINSYYRPKNGFLNISPAVNGSR
                     TVVQQWGHSPVTDGDELRLLRIAYRRALGYDEMPSADLLDDLAREIKKQIVATEDLKV
                     ESSLFYREMFSSKNKSYAALDEGTDSTVGDKKFFDSTGDPARAARKTPLAREVASEVN
                     EIVDELKKIHPGWYKVGRKHDIPKNASYVAHYKDVYVWVEPEGVDGLTEFTLSVL"
     gene            complement(5871..>7094)
                     /locus_tag="VHS97_23655"
     CDS             complement(5871..>7094)
                     /locus_tag="VHS97_23655"
                     /inference="COORDINATES: protein motif:HMM:NF020340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1793 domain-containing protein"
                     /protein_id="HEX3451270.1"
                     /translation="CAAIAKMEGTAEFSSTWWPQLTRWEAYLEKYGEDPENQLCTDDF
                     MGHLAHNANLSVKAILAIGAYGELCRMRGDQASAKKYKNLAKEYAQHWIKVAADGEHY
                     RIAFDKPNTWSQKYNLVWDELLGLDIFPAEVARKEVAFYKTRLERFGVPLDSRTRLTK
                     TDWSLWIATMAKVPADFEAMVSPIHDYLNQTTARLPFVDSYMTNNAQSDGMRARPVIG
                     GVFIKMLADVATWKKWAARDRARVGNWAPAPAPPRVTEVVPTSEHKPVVWRYSFTKPG
                     ADWARPDFDDSKWKQGPGGFGKSGTPGAVIGTTWDTPDIWLRREIILPDGATPANIQL
                     RVYHDEDLEVYLDGVLAASQSGYLTAYDQVEIRPATLKLLTSGKKFVLAVHCHQTSGG
                     QGVDVGLVDVVEAGR"
BASE COUNT         1164 a         2153 c         2203 g         1574 t
ORIGIN      
        1 atcttggtgc agcgtggacg cacggtctgc ttgattcagc tttcacaggc ggtgatcaat
       61 tcggcggacc tgctcgttgt cggattcctg agtgttgcga gcgacgtcgg ccggtacggg
      121 gcaccgcacc ggatggtgac ggcattcctg acgttcggtc taatcctcca gcaagcggcg
      181 tttcccacgc tctctcgatt gtggcgccag acggcgggtg ccgggcaaga agcgctcgac
      241 tcgctggttg aggtcttgat gaccgggctg gtgccggtcg ccgtcggctg tacggtgctg
      301 gccgatccgc tggtgcacct gtttctgccg aatgggtatg cgggggccgg cttcttgctg
      361 gccctgggga tctggcgagc ccccctcttg atattggctt atctgtatca gatcacgctt
      421 atcgcgctca atcgcgagac tgttggagtg cggaccctgg tggccggcgc cctggtgatt
      481 gtccctttgg ttgccctgct gcggttgagc tttgggctgc cgggagccgc ggccggcgtg
      541 ctgttggtcg gtcttgctct ggtcttggca ggttacagtt gcctggctcg cgagggccgg
      601 cagcccgcct ggcaccacca cctggcgcgg ccgattttag cgtcactggc gatggtaccg
      661 gcttgcatgg cgctcaagca ttatcatgtg ctattggctg tagtcggtgg cgcagtcact
      721 tatagcgccg tctggctggc cttggggggc ttgcgtcaca ctcgactatg gggccgcgcg
      781 ctgcggccct tggcacgttc cgggacgact tcgtgattct taagggcgtc aactcgtggg
      841 caatccccac agccgatcct gagacatcta ctgcatctga gacgcgaagc gacatggagg
      901 tcgccgatga aattccgaat gatcacgaca ttcttcgggg agcacagctt cggtggtgat
      961 gctgcatatg tcgatcgact cagccaggcg ctctgccgcc gtggacatga agtccatgtg
     1021 tattattgtg tggatgcctt taacacagtt cgcggcgacc accctctacg gccctacaca
     1081 cctccgcctg gaattcacct ccatcctctc aagagccggt tcggtattct ttcgcccctg
     1141 gccacgcaga cgaccggcct gccactctta aagacggcgg aactgcgtga ggcactcgac
     1201 gaccccgaaa ccgatgtcgt gcactttcac aacatttcgc tcgtaggtgg gccgggcgta
     1261 ttggggctcg gagccaatcg acgcgcagtc cggatcatga ccgcgcacga acattggctc
     1321 gtctgtccga tgcatcttct ctggaagtac gatcgcaagc cgtgcgacgc ccccacctgt
     1381 gtgagttgct gcttgcaggg ccggcggccg ccccaggtct ggcgagcaac gagagcaatc
     1441 gagaggggct tgcgaaacct cgacgcgctc gtcttcccca gtcgccacgc tctggaagag
     1501 catcggcgac gcgggttggc ggcgcacgtg cccttggttc atgttcccta cttcctgccg
     1561 gatgactggt cggcgggagt ggaacaagcg cctcgcacag tgcctgatcg cccttacgtc
     1621 gccgccgcgg gaaggttggt gaaaatgaaa ggatttcagc atttgattcc gttgatgcga
     1681 tttcttcccg aggtcgacct gcatattgcg ggaaccggtc cgtacgagcc ggcgctgcgc
     1741 gcgatggcag cggaactgcc gaatgtgcgt ttcgaggggc tcttgggagg acaagcactt
     1801 gccgcccttt ttcgacacgc ccgcgccgtc gtggtaccgt cgctcttccc ggaaaccttc
     1861 ggctacgttg tgctcgaggc cttcgcagtg ggaacaccgg tcatcgtgca tgaagggggc
     1921 ggcgctcttt acgagaccgg gtttttgagt ggcggcggac tggcctatcg gtccgacacc
     1981 gagctgctga ccgcgatgcg caggatggtc catgatcgcg ccctgcacga cgagctcgcg
     2041 gcgcgcggtg tcgccatgcg catgggccca tggtccgaga ccgagcatgt ggacgagtac
     2101 ctgcgtctca tcgaccagag tcagtccgca cgccagcgca agccgttccc ccgcccgcat
     2161 tccctcaacc ggcccaatgc ggctcctgcg cgtgttgaca gcctgccgtg gagcgaactt
     2221 gggaacgaac caatgaaggc cgcaggtatc aatcggccgc atggcatgat ctaggagccg
     2281 ccacccgtgc gagagcgccg atggcgaccc accgcgctgg tttcggcctt gacactggtc
     2341 attttggccg gcgtttgctt cgcgcggctg gtggttcaac cggcggcaat catcgtcgac
     2401 gaagagcggc ccagcctcga tcacgccaac ccgggcgagc cacgcggtat cggcaacgac
     2461 gctgcgtttt tgtttctgcc gcaccacctc tcgatcgcca aggtcattcg cactttcggc
     2521 cacgtaccca gttgggacgt tcgaggattt ggcggacggc ccttgatcgg taatccgcaa
     2581 gggggcacgt tttatccgcc ggtctggttg gtctggtggt caggtgcccc ggccgcgctc
     2641 ggatggctga ccgtcgggca tctcctctgg ggcggggcgg ggatgtacgt gctcttgcgg
     2701 tctctcgggg caggcccatg gggcgcgacg atcgcggcag cctcgtacca agcatcgcca
     2761 ctgctcttgg ctcacacctt cgaagggcac tatccgcatg tctgggccgc ctgctggttt
     2821 ccatgggctt tctgggcttt ccacgaccac cggtcgggac acgtgcgcgg acgtttactc
     2881 ttgccgatca tcctcgtctt cacttatttg acaggccatc ctcaggaatg gttgctcctg
     2941 gttctggcac tgacagcctg gtgcctgggc gcggcctgga gattatggcg cgctcagggg
     3001 ccgcgcgaaa gcgtggctca ggttgtctgg tgggctggta cgctggcgtt atcgatcggc
     3061 atcgcggccg tcgagatagc gccggagctt gccgtccgcc cctggcttac gcgcaactcg
     3121 gacgcgccgg cgggaatcga gattccccgg cgatatcacc tcggcgctgg caatctcttg
     3181 cagttgctca gtccgaccgc actcggtgga ccatccgact atttcggcga cgacaactac
     3241 tgggagaccc tcttctctat cggcttggtg ccgctggttc tcgccgtaac cgcggccgtg
     3301 tggcatcgtg accgcaaaca gattcgtggt tggctctggc tgatggggct ggcgatctgg
     3361 ttcgcctgcg gacgccgcct gttgttcttt gtgattgctt atctgacggt gccgggcgtc
     3421 agctggttcc gagtgccggc gcgggctctc tttcttgcca atctcgccgg cgcggcgttg
     3481 gccggtctgg gggttgaaac tcttcaaatt cacaggggtc gctctcagtt ctggcacaag
     3541 tacgctgtcc gattcgcatc ggtttttgtg atcggtctgg cgtgcctatt ctcgatccgg
     3601 agttatcatg gatcagacag ttcatcgcgc attgccctgg cggcggggcg ggttctcgac
     3661 gatgcgtctt tcaggtcggt gctcgcgacc atcgcggccg tgatcctggc gggatgcctg
     3721 gttcgcggct ggcgaaatcc gctcctggct ggtcgcctgc ttgggctctt ggtgctatgc
     3781 gagctcggct ggaatggggt ttcgctgatc caggtcgctc gcgcagatcc gttcctgggc
     3841 catcatcctg taagtgcggc gctgactcaa tttcgtcatg attcaccggg cgccggccgg
     3901 cttcgcgtca aagcacgcga tctgttttac agcgatctcc aggctgcctc gattgacgtt
     3961 gaaaagacca acatcaacga tgtcttccag ctcgcccacg cttcccggct ttatgcacag
     4021 ctctacccgg tggcgtctcg tctccgccgt tcgatcgacg atcacatgca cgaggcggtc
     4081 gaataccacc gtaggcaaat ccggcaggca gtcttcgatc gcttcagcgt cgcttacctt
     4141 gtttctgacc ggtacgaatc ggatccgggt tggccggtcg cggctcaagg cgaatggcat
     4201 ggctctccgt gggtgattca gcaaaatacc tccgcgctgc cgcgcgcata tgtcgtgccc
     4261 accgcggcaa tagtctccga ggacgaatcg agccctcgtg cgcgattcct tgacgttgat
     4321 ccccgcgaat cggttttgat gaatgacgat cctctcgttc acctcgccga tcgaccgaga
     4381 cagcccttta cccccgccca atggacctcg ctcgatcccg atcacccggt catcaccgta
     4441 acgacgcacg gtgccggcct actggtagtg agcgacacgt ggatgcccgg atggacggcc
     4501 cgtgtggacg gcgcgaaaac tccaatttac cggggaaata tcgcccagcg ggtcatcccg
     4561 ctgtggcaag cgggccgcca taccatcgag atggactatg ttccgccggg gcttgcactg
     4621 ggatgtatcc tctcggcact ttcacttctg agctgggctc tgatgtgcgg ctctgcaatc
     4681 gccaagtcac tcaagaatga agtgccccgc cattgtggcg actttcgggt cttgacgact
     4741 agaacgacac gtcgaagccc ggcgagtagt tcacacctcc ggatcccagg ctgatcgggg
     4801 gcggcgggtg aatagtcgaa gccatctaca ggacggacag cgtgaattcc gtcaggccgt
     4861 cgacgccctc tggttcgacc cagacgtaca cgtctttgta atgggcgacg tacgaggcgt
     4921 ttttgggaat gtcgtgtttg cggcccacct tataccagcc tgggtgaatc ttcttgagct
     4981 cgtcgacgat ctcattgacc tcgctggcga cctcgcgcgc aaggggtgtc ttccgagcgg
     5041 cacgcgcggg atcaccggtc gagtcgaaaa acttcttgtc gccgaccgta gagtcagtac
     5101 cctcgtcgag cgccgcgtag gatttatttt tggagctaaa catctctcga taaaagagtg
     5161 atgattcgac tttcaggtcc tcggtggcga cgatctgctt cttgatttcc cgcgccaggt
     5221 cgtcgagtaa gtcagcggag ggcatctcgt cgtatccgag ggcgcggcgg tacgcgatcc
     5281 gcagcagccg gagctcgtca ccatcggtaa cggggctgtg gccccactgc tggacaaccg
     5341 tccgcgagcc gttgacggcc ggcgagatgt tcaagaaccc gttcttgggc ctgtagtatg
     5401 aattgatccc gatgccgagg gtcccttggg ccgagtcggt catctgggtc gtgccgcccg
     5461 tgatgttggc gtgccacggc attgtccccg cgttggcggc cagcgtcgcc agattctcca
     5521 ggaccatttg gtattgcaac tcggtcatgg tgttcgcctg gctgactgtc gaattccgca
     5581 gcgcagaatt cgtacagccc gtcccgagtg acagcgtgcc cagcatcaaa atcgtggcca
     5641 cgcgcatcgt caggtctcaa gtcgcgagaa ttgatacgga ggatcctgcc tattatatcg
     5701 accgcgcgag atttggcact tgagtcgaat gcgcgggccg atagaggctc gcgcgaccgg
     5761 agaaattaca tcctgggagt gggcgggtaa gccctgactt cccgtcggat atcgcttatc
     5821 tcggcggcgc aatcgagtgc caagacccgt gtcacttagc gttcgtcgat ctaccgtccc
     5881 gcttcaacca catcgaccag gccgacgtcc accccctgcc ctccactggt ttgatggcaa
     5941 tgcacggcga gaacgaattt ctttccagaa gtcagcaatt tgagtgtagc cggtcgaatc
     6001 tcgacttgat cgtacgctgt caagtaaccc gactggctgg cggcgaggac gccatcgagg
     6061 tagacctcga ggtcttcgtc gtggtagacg cgaagctgga tgttagcggg agtcgcaccg
     6121 tcaggcagga ttatttcgcg gcgcagccag atgtctggcg tgtcccaggt cgtgccgatg
     6181 acggcgcccg gcgtgccgga cttgccaaac cctcctggtc cttgtttcca cttgctgtcg
     6241 tcaaagtcgg gcctggccca gtcagcgccg ggtttggtaa atgagtagcg ccatacgacg
     6301 ggcttgtgct cggacgtcgg cacgacttcg gtgactcgag gcggtgcggg ggccggcgcc
     6361 cagttgccga cgcgcgctcg gtcccgtgca gcccacttct tccaggtcgc aacatccgcg
     6421 agcatcttga tgaacacgcc gccgatcacg ggcctggcac gcatgccgtc gctctgagcg
     6481 ttgttggtca tgtacgagtc tacgaacggg agccgcgcgg tcgtctggtt cagatagtcg
     6541 tggatcggcg agaccattgc ctcaaagtcg gccgggactt tcgccatcgt cgcgatccag
     6601 agactccagt ccgtcttggt gagacgcgtg cgcgagtcga gagggacccc aaatcgctcc
     6661 aaccgggtct tgtaaaaggc gacttccttt cgtgccactt cggcaggaaa aatatcgagg
     6721 cccaagagct cgtcccacac aagattgtac ttctggctcc atgtgttcgg cttgtcgaag
     6781 gcaattcgat agtgctcgcc gtcggcggcg acttttatcc agtgctgtgc atattccttc
     6841 gcgagattct tgtacttctt tgcggacgct tgatcgccac gcatgcgaca gagttcaccg
     6901 tacgccccga tggccaggat tgccttgact gacagattgg cgttgtgggc cagatgaccc
     6961 atgaagtcat cggtgcagag ttggttttcg gggtcctcgc cgtacttttc cagataggcc
     7021 tcccaccgag tgagctgggg ccaccatgtc gacgagaatt cggcggtccc ctccatcttg
     7081 gcgatggcgg cgca
//
LOCUS       DATAQL010001374         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5178734, whole genome shotgun sequence.
ACCESSION   DATAQL010001374 DATAQL010000000
VERSION     DATAQL010001374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5178734"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VHS97_23660"
     CDS             complement(<1..864)
                     /locus_tag="VHS97_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451271.1"
                     /translation="MAARPSIRQGIYDLLSAVEIDTKEEGRTHVEPWMSQQLVINAVA
                     KGLDEGVHEFVILKCRQVAITTVCSVIELFWALANPGVQGAIIADRTDNLERLRRIFA
                     ALLETLPPEWRSTEHRLTQNNRNGMAFANRSVIDLMAAASNPDLGASRALNMMHATEC
                     GQWKSLAGVESLKASLARVNPTRLYVWESIANGFNWFYNHCQQAKVDRHMRFIFIGFW
                     ANPTYSIPKSDPDYKTFWDGGHLTDEEIEKARFVKRTYGVTVKPEQIAWWRRESEVRA
                     EEYMLRHYPWNE"
     gene            complement(848..1063)
                     /locus_tag="VHS97_23665"
     CDS             complement(848..1063)
                     /locus_tag="VHS97_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451272.1"
                     /translation="MPKAKAFTTEPNDNGHQLDPIVDGLLDRLPAPGDVFMPADRQLW
                     IKIMTDVFQLIYLDKEPETTETDGGAA"
     gene            complement(1097..1618)
                     /locus_tag="VHS97_23670"
     CDS             complement(1097..1618)
                     /locus_tag="VHS97_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451273.1"
                     /translation="MDNGGRAYREEVFAIEREVKREADILRLAEVNETTPETILQGHL
                     SNRWKRRDGDIYRAAAAAWVDFIPDPGWVDNSIRARIMNIGLSSTLQVIGDISAPLPA
                     NQKARVGILPEGYRPARTYRTSGAVVGGPTGITWAYAIWEITATGEVWTVWGIGSSPA
                     NSTGSINAIVPMD"
     gene            complement(1731..>1950)
                     /locus_tag="VHS97_23675"
     CDS             complement(1731..>1950)
                     /locus_tag="VHS97_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451274.1"
                     /translation="LTFFGATAADAQITNCFRNGFGISCYTNPTYPTYQGPSVAQQFL
                     DGFAQGRMERHNRELQLENERLRRQLGQ"
BASE COUNT          320 a          567 c          623 g          440 t
ORIGIN      
        1 ttcgttccac ggataatgtc ggagcatgta ctcctcggcc cggacctccg attcgcgtcg
       61 ccaccatgcg atttgctccg gtttgacggt cacgccgtag gtccgtttga cgaagcgggc
      121 cttttcgatt tcctcgtcgg tcaggtgtcc gccgtcccaa aaggtcttgt agtcgggatc
      181 tgacttgggg atcgagtagg tcggattcgc ccagaagccg atgaagatga accgcatgtg
      241 acggtcgacc ttggcttgct ggcaatggtt gtaaaaccag ttgaagccat ttgcgatcga
      301 ctcccagacg tagagccggg ttgggttgac gcgcgccagc gaggccttga gcgattcaac
      361 gcccgccaga gatttccact gcccgcactc ggtcgcgtgc atcatgttga gcgcgcggga
      421 cgcgccaagg tccgggtttg aggccgcagc catgaggtcg atcacgctgc ggttggcgaa
      481 cgccatgccg ttgcggttgt tctgggtcag ccgatgttcg gtcgatcgcc actcgggcgg
      541 cagggtttcg aggagcgcgg cgaagatgcg ccgcagccgt tcgaggttgt ccgtcctgtc
      601 ggcgatgatc gcgccctgca cgcccggatt ggcgagcgcc cagaacagtt cgatcacgct
      661 gcacacggtg gtgatcgcga cttggcggca cttgaggatg acgaactcat ggacgccctc
      721 gtcgagcccc ttggcgacgg cgttgatgac gagctgctgg ctcatccacg gttcgacatg
      781 ggttctacct tcctctttgg tgtcgatttc gaccgcgctt aagaggtcgt agatgccttg
      841 ccggatgcta ggccgcgccg ccatctgtct ctgtcgtctc cggctccttg tcgaggtaga
      901 tcaactgaaa tacgtcggtc atgatcttga tccagagctg ccgatcggcg ggcatgaaca
      961 cgtcgccggg cgcgggcagg cggtcgagca agccgtccac gatcgggtcc agttgatgac
     1021 cgttgtcgtt gggttcggtg gtgaaggctt tggctttcgg catttagctc ctcccaaaaa
     1081 ctgagctgtc gcacgttcaa tccatcggga caatcgcgtt gatcgagccg gtgctgttcg
     1141 ccggactgct gccgatgccc cacactgtcc acacctcgcc ggtcgccgtg atttcccaaa
     1201 tcgcataggc ccaagtgatc ccggtcgggc cgccaacgac ggccccgctc gtgcgatagg
     1261 tccgggccgg tcgatagcct tccggcagga tgccgacgcg agccttttgg ttcgctggca
     1321 acggcgcgga gatgtcgccg atgacttgca gcgtcgagga caagccgatg ttcatgatcc
     1381 gggcgcggat gctgttgtcc acccatccgg ggtctggaat gaagtcaacc catgcggctg
     1441 cggcggcccg atagatgtcg ccgtcgcgcc gcttccagcg gttcgacagg tggccttgca
     1501 ggatggtttc gggggtggtt tcgttcactt cagctagacg caggatgtcg gcctcgcgct
     1561 tcacttcgcg ctcgattgca aagacctcct cgcggtatgc gcggcctccg ttgtccattg
     1621 caacctcctg tggtatttgt gcgaagcgcc ccgccgtgac cggggcgtgg tcccctaatg
     1681 tgtgtctttg gtggtcgcca tcggaggtag ctccccgatg gcgatccttt ttactgtccg
     1741 agttgacggc gcagccgttc gttttccagt tgcagttccc gattgtggcg ttccatgcga
     1801 ccttgagcga agccatcgag gaactgttga gccaccgatg ggccttgata ggtaggatag
     1861 gtaggatttg tgtaacaaga tatgccgaag ccgttgcgga aacagttcgt aatttgcgcg
     1921 tcagcggcgg tggcgccgaa gaacgtcaac
//
LOCUS       DATAQL010001375         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1057832, whole genome shotgun sequence.
ACCESSION   DATAQL010001375 DATAQL010000000
VERSION     DATAQL010001375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057832"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VHS97_23680"
     CDS             <1..367
                     /locus_tag="VHS97_23680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3451275.1"
                     /translation="AMSNQIVWVDIPVLDLDRAIRFYSAVLGGEVQKQEYPGMAIGLL
                     PGYDSDVSGCLFTKADERPTPTGPLIYLNAQGRLDEAVAVVEPNGGSVLQPPHQIGPH
                     GFRAVILDSEGNRVALHSR"
     gene            complement(469..2970)
                     /locus_tag="VHS97_23685"
     CDS             complement(469..2970)
                     /locus_tag="VHS97_23685"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX3451276.1"
                     /translation="MSQSSEATQQLSADERRRLLAEMLQKKASRSDERPLSFGQERLW
                     LMARLDPGSSLYNIAVAYRIEGPLDLTVLKESIERVAERHEVLRATFPAGKGQPVQKV
                     ALEVPSVFAVREHGDLTEDWRTTSVPQFAVEEAKRPFDLTQGPLWRVSVFRKSDEEHY
                     IVLVMHHIVSDAWSFYILCHELAEFYDAAVLGRPARLAELPIQYADFGQRQRQWLSGS
                     VFEQQLAHWRAHLQGNIPRLQLPADRPGSTATYHRGAFQTLAIPTAVSQTLGELSRRE
                     SATMFMTLLAGFQVLLHQYSGQEDLVLCTPASGRHRSQTKELIGYFNNILPMRFDLSG
                     DPTFTQVLRRTRRVALDAFKYQDLPFQIIADSPNLKALSLSRVLFSVDIEWPPKLALK
                     GLACEAWAVRTDTADFDLTVSLWMEGEELRGVFEYKTELFNDGTITRAIADYQELLAT
                     VAAKPETAISSLPARTKPDAEVHTVPLGRRQTAYHPPQSPTELRIINELEDLLGIHPI
                     GIEDDLLELGAPSLAVARLSERLRRIFGVELPLAALFQARTVAKIADLVRCGSSGLSR
                     SALAPIQPAGVHPPLFLCEGLGIYYPLTRHLDKEQPVYGLVTEIARADHPRVEDLAAW
                     YVAEVRAVQPEGPYFLGGLSFGGIVAFEMAQQLCAVGQEVALLALFDTPTPWAFTAKP
                     LLPRLAGHMINLRRFGFRYVQKKLGRRLKSLQGIVAHRRGPPADSQSDIIANTDQLRR
                     VFSTTADQYELRSYPGRAALFILSERDGMSDSLFDPALGDIDPQLGWGRVVAGGVEVY
                     EVPGEHVSIFREPNVRSLAEKLTNCLEMARETALK"
     gene            complement(3042..>3439)
                     /locus_tag="VHS97_23690"
     CDS             complement(3042..>3439)
                     /locus_tag="VHS97_23690"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3451277.1"
                     /translation="SIIEWLVQWIARELGLPPVEIETGKSLLNYSLSSVTAMMLVGDL
                     EEWLGLTLPPTLVWDYPSIDSIADYLIEQLGTSVAGTPLSNGAAASADWQLPGTGTAP
                     MPPLAEMSDQEVTALLSQLIADERAGSSL"
BASE COUNT          696 a         1062 c         1033 g          648 t
ORIGIN      
        1 cgctatgtcc aatcaaatcg tatgggtcga tattccagtt ttggacctcg atcgcgcgat
       61 ccgcttttac tcggccgtcc tggggggcga ggttcaaaaa caagagtatc cgggcatggc
      121 aatcggcctc ttgcccggat acgacagcga cgtcagtggc tgcctgttta ccaaggccga
      181 cgagcggccg actcccacgg gccccttgat ttacttgaac gcacaagggc ggctcgacga
      241 agccgtggca gtggtcgaac ccaacggcgg ctcagtgctc cagcccccgc accagatagg
      301 acctcacggt ttccgggctg tcatcctcga tagtgaagga aaccgagtgg cactccattc
      361 acggtgaagc cgtcgatcag aagtgtatct accaggtggc gcggggcttc ttcgattccc
      421 tgcgcaccag agtgactcaa tcgaccagcc tcaacgatcc attcccgatt acttcagagc
      481 cgtctcgcgg gccatctcca ggcaattcgt cagcttctct gccaggctcc gcacgtttgg
      541 ctcgcggaat atgctcacgt gctcaccggg cacttcgtag acctccaccc ctccggctac
      601 cacccggccc catccgagct gtggatcgat atcacccagg gccgggtcaa acagagagtc
      661 actcatacca tcgcgctcgg aaagaatgaa aagcgcggcc cggcccggat aactgcgcag
      721 ctcatattga tctgcggtcg tgctgaagac acgtcgcaac tgatcggtat tggcgatgat
      781 atcggattga gagtccgcgg gcggccccct cctgtgggcg acaatcccct gcaaactttt
      841 cagccgcctc cccaacttct tctgcacata gcggaagccg aatcggcgca agttaatcat
      901 gtggccggcc agcctcggga ggaggggctt tgctgtaaag gcccatgggg tcggtgtgtc
      961 gaaaagcgcc aagagcgcga cttcctgacc aacggcgcaa agttgctggg ccatctcgaa
     1021 cgcgacaatc ccgccgaacg acaatccgcc caggaaatag ggcccctcgg gctgcacggc
     1081 ccggacctcc gcgacatacc atgcggccag gtcctccacg cgcgggtggt cggcccgggc
     1141 gatttcggtc acaagcccgt agacgggttg ctccttgtcc aggtggcgag tcagcggata
     1201 gtagataccc aggccctcgc agaggaataa ggggggatga acgccagccg gctgaatcgg
     1261 cgccagggcg gaacgcgaaa gccccgagct cccacatcga accaggtccg cgatcttggc
     1321 aaccgttctt gcctggaaaa gtgcggcgag cggtagctca accccgaaaa tcctgcgcag
     1381 gcgctccgaa aggcgagcga ccgccagcga cggtgcccct agctcgagga gatcatcctc
     1441 gatgccgatc ggatggatgc ccaggaggtc ctccagctca ttgataatcc gcagctcggt
     1501 cggggattgc gggggatgat atgcggtttg gcgccggccg aggggtaccg tgtgtacttc
     1561 ggcatcgggt ttggtcctgg ccggcaggga cgatatcgcc gtctcgggct tcgcggcgac
     1621 tgttgccagc aattcttgat aatcggcaat cgcccgggtg atcgtgccgt cgttgaacag
     1681 ctccgtcttg tactcgaaca ccccccgtag ctcctcaccc tccatccaca acgatacggt
     1741 caggtcgaaa tccgccgtgt cggtgcggac cgcccacgcc tcgcacgcga ggcccttgag
     1801 cgcgagcttt ggcggccatt cgatgtcgac ggaaaacaag acacggctca gcgataatgc
     1861 cttcaggttc ggtgaatcgg caatgatctg aaagggtaga tcctgatatt tgaatgcatc
     1921 taacgcgact ctccgcgttc ttcgaagcac ttgggtgaag gtgggatcgc cgcttaggtc
     1981 aaaacgcatg ggaaggatgt tattgaagta accgatcaac tccttcgtct gggagcggtg
     2041 gcgaccagag gccggcgtgc agagtaccag gtcttcctgg ccgctgtact gatgcaggag
     2101 gacttgaaag ccggccaaaa gcgtcataaa catcgtcgcg ctttcgcgcc ggctcaactc
     2161 gcccaaggtc tggctcacgg cggtcgggat cgcgagcgtt tgaaacgcgc ctcgatggta
     2221 agtcgcggtc gatccggggc gatcggccgg caactgcagc ctggggatat tcccctggag
     2281 atgcgcccgc cagtgagcca actgctgctc gaataccgag cccgagagcc actggcgctg
     2341 ccgctggcca aagtcggcat actggatcgg cagctcggcc aggcgcgcag gccggcccaa
     2401 gacggcggca tcgtaaaact cggccagctc gtggcataag atgtagaatg accaggcatc
     2461 agagacgatg tggtgcatga caaggacgat atagtgttct tcgtccgact tccggaagac
     2521 cgaaacgcgc cacagcggcc cttgtgtcag gtcaaacggc cgtttcgctt cctccaccgc
     2581 gaactgagga acgctggtcg tgcgccaatc ttcggtcaaa tctccgtgtt ctctgaccgc
     2641 gaacaccgat gggacctcga gggcgacctt ttgcaccggt tgccccttcc cggccgggaa
     2701 tgtcgcccgt aacacttcat gacgttccgc gacgcgctcg atgctctctt tcaatacggt
     2761 caagtcgagg ggaccctcga tacgataggc gaccgcgata ttgtacagcg agctgccggg
     2821 gtcgaggcgc gccatcagcc agagccgttc ttgcccgaac gagaggggac gctcgtccga
     2881 gcggcttgcc ttcttctgaa gcatctccgc gagcagccgg cgcctctcat ccgcggacag
     2941 ctgctgggtg gcttccgaag actgactcat caactcctcc tgtgaccctc accgtcaggg
     3001 tcttttgatc gcatcgtcct gggccgacca agaccgagtc ttcaaagcga agaaccagcg
     3061 cgctcgtcgg cgatcaactg actgaggagg gcggttacct cctgatcgga catttcggcg
     3121 agaggaggca tcggcgcggt gccggtgccg gggagttgcc agtcagcaga tgccgctgcc
     3181 ccattgctga gcggagtgcc cgccacggag gtgccgagct gctcgatcaa gtagtcggca
     3241 atggaatcga tcgaagggta atcccagacc aaggtcggag ggagcgtcag acccagccat
     3301 tcctccaggt cgcccaccag catcatggcg gtgacagagc tcaagctgta gttcagcaga
     3361 gacttgcccg tctcgatctc cacgggaggg agtcccaact ctctggcgat ccattggacc
     3421 aaccattcaa tgattgaat
//
LOCUS       DATAQL010001376         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5025667, whole genome shotgun sequence.
ACCESSION   DATAQL010001376 DATAQL010000000
VERSION     DATAQL010001376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5025667"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /gene="ltrA"
                     /locus_tag="VHS97_23695"
     CDS             complement(<1..1279)
                     /gene="ltrA"
                     /locus_tag="VHS97_23695"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX3451278.1"
                     /translation="MHGHEKSDPEIVAIRTNAVGMSREKPANNAAPTAAELVERRAGT
                     KGNAGQQSTLRAQDRAGVSQALERIRQAARHNKKERFTALLHHVNSETLRLAFNALKK
                     DAAPGVDGTTWSDYEVDLERRLEDLHDRVHRGAYRPQPSRRVYIPKADGRQRLLAVAA
                     VEDKIVQGAVAMVLSAICEVDFLGFSYGFRPGRGQHDALDALVVGIDSRKVNWILDAD
                     ISSFVDTVDQQWMIRFVEHRIGDPRIIRLIQKWMKAGVLEDGEFKASDRGTGQGSVIS
                     PLLANIYLHYAFDLWANRWRQREATGDMIILRYADDTVVGFEHEADARRFLDMMRARL
                     EEFALSLHPEKTRLIEFGRHAAADREQRGLGKPETFNFLGFTFICSKSRKGRFLIIRK
                     TRRDRMMAKLLELKEEMRRRMHWPISEQGEWLKQ"
BASE COUNT          300 a          622 c          509 g          389 t
ORIGIN      
        1 cctgcttcag ccattctccc tgttcggaga tcggccagtg catgcgtcgt cgcatttcct
       61 ccttcagttc caggagcttc gccatcatac ggtctcgccg tgttttcctg atgatcagga
      121 agcggccctt gcgggactta ctacagatga aggtaaaccc caggaagttg aaggtctccg
      181 gtttgcccag cccgcgctgc tctcggtcgg ctgccgcgtg gcggccgaat tcgatcaggc
      241 gagttttctc cggatggagc gacagcgcaa actcctccag gcgcgcgcgc atcatgtcga
      301 ggaaccgacg ggcgtcggcc tcgtgctcga agccgacgac ggtatcatcc gcatagcgca
      361 ggatgatcat atcgccggtc gcctcccgct gtcgccagcg attggcccag aggtcgaagg
      421 catagtgcag gtagatgttg gccagcagcg gtgaaatcac cgagccctgg cccgtccctc
      481 tgtcgctggc cttgaactct ccgtcttcaa gcacgcccgc cttcatccat ttctggatca
      541 ggcggatgat gcgcggatcg ccgatccgat gttcaacgaa acgaatcatc cattgctggt
      601 caaccgtgtc gacaaacgaa ctaatgtcgg cgtctagtat ccagttcacc ttccggctgt
      661 cgatcccaac caccagtgcg tccagcgcat cgtgctggcc acgtcccggt cggaacccat
      721 acgagaaccc gaggaagtct acctcgcaga tcgcgctcag caccatcgcg accgcgcctt
      781 ggacgatctt gtcctcgact gcggcgaccg cgagtaggcg ctgccggccg tccgcctttg
      841 gtatgtacac ccggcgtgac ggttgaggcc gatatgctcc tctgtgcacc cggtcgtgta
      901 ggtcctcgag cctgcgctcc aagtctacct cgtagtccga ccaggtcgtc ccatccactc
      961 cgggggccgc gtcctttttg agggcgttga atgccagacg cagcgtttcg gagttgacgt
     1021 gatggagaag cgcggtgaac cgctccttct tgttgtgcct cgcggcttgc cgtatgcgct
     1081 ccagcgcctg ggacacgcct gcccggtcct gagcccggag cgtgctttgc tggcccgcat
     1141 ttcccttggt ccctgccctt cgctccacca gctccgccgc ggttggggcc gcattgttcg
     1201 ccggcttctc ccggctcatg ccaacggcat tcgtccggat cgctactatt tcagggtctg
     1261 acttctcgtg tccgtgcatc gtcggctacg gctcctcgcc ttcccgacgc gggccgagca
     1321 gcccgagggc cgcccggtca gacatgagat ctcccaggtt ccgacctgtt ccttttatgc
     1381 gtgatggggt ctttgaccac gacggagtgt cggcgccttg catggcggca tcgcacatgt
     1441 tgccttcggc actgggggac ggcctcggcc tccgtcattt tcaaactttc gcggctcaat
     1501 atcccacccc gcataattgc tgtgtacgct tcatggccgc cgtcgccggc gggccacgca
     1561 acactcgcta ccgggcggtt gctagcccta cccggaccgg actctcaccg gctggaccag
     1621 atcagcttat cctgacgcac cccccaatct tgagccagcc cccaacgaca gcagacccca
     1681 cggggctatt cccacctcag tctgaaatcg atgccttgat caagaacatc aaaaccgttc
     1741 caaccaccaa tgccatcgcg tgtgtggcca tggaattctc cacccgtggc aagcgcggtg
     1801 ctccgccaag tgccgtgccg
//
LOCUS       DATAQL010001377         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1821404, whole genome shotgun sequence.
ACCESSION   DATAQL010001377 DATAQL010000000
VERSION     DATAQL010001377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821404"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VHS97_23700"
     CDS             complement(<1..585)
                     /locus_tag="VHS97_23700"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEX3451279.1"
                     /translation="MNAKLFRAAGFPMLLAAVLVASAATPTSRTTLQGSIPPWANDRN
                     LIGSADPNGDVGFRVYLGWTDPTGAEALARAVSDPTSSSYGKYMTPAKFRSRFAPAAS
                     DVAKVQNWLRSQGFDVQYAPANNHYISAEGTIAQAQNAFGVQFGSYAVRGQSVRSPTA
                     NVSIPGSLASIVKGIVGLDDSSQFVQTHLKVDKNA"
     gene            1179..>2831
                     /locus_tag="VHS97_23705"
     CDS             1179..>2831
                     /locus_tag="VHS97_23705"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX3451280.1"
                     /translation="MTKHLRALRFAFCLALTVASGQAALAADPPAQPQDAQYTKLIGD
                     YLVDKRITTELVDHMPASDSVPSPLKFFGRIPGTPGELTYAKDIERYYQELAKSSPRA
                     RFWKLGTSEEGRDQVVLAIADEETLKNLDKYKADLAALADPRRTTEEQAKAIIHTSKP
                     VYWITSGIHSPDTGGPEMLIELAFRLMVEETPFIQNIRDNVITFITPVVEVDGREKEV
                     DTYYYGKKTGKPRPPLMYWGKYVAHDNNRDGMGQFLKLTQNITKGVLEWHPTVLHDLH
                     EAQSYLYVSTGTGPYNPSLDPVQSDEWWLLAETEVSEMAKRKVPGVWTWGFYDGWVPN
                     YLFWIAITHNSFGRFYEVQSYGPDIQKGLKLAPTVTSHEWYRPNPPLPSIDWGPRNNT
                     NIQESAILFALNRVAKDHDLYLENFWIKSKHAVEAGRSGAVNAWVIPAAQRAPLNVAA
                     MINDLRHQGVEVNTASEAFTAGAVSVSTGDFIVRADQPYRTLADMYFSVQDYPAPNPR
                     PYDDTGWTMQYMRDVKLSPVHDPEILNKSMSLLAADVTPKGAI"
BASE COUNT          590 a          857 c          826 g          558 t
ORIGIN      
        1 agcgttctta tcgaccttca aatgggtttg tacgaactgg gaactgtcgt cgagtccgac
       61 gatgcccttg acgatcgatg cgagcgagcc cgggattgaa acattggcag tcggcgaccg
      121 cacgctctga ccgcgaaccg cgtaacttcc gaattgaaca ccgaacgcgt tttgcgcctg
      181 ggcgatcgtg ccttccgcgg atatgtagtg attgttcgcc ggggcgtact ggacatcgaa
      241 tccctggctg cgcaaccaat tttgcacctt ggcgacgtcg ctggcggcgg gtgcgaagcg
      301 gctgcggaat ttcgcgggcg tcatgtattt gccgtacgac gaactggtgg gatctgacac
      361 agcccgcgca agggcctcag cgccggtcgg atcagtccag ccgagataga cgcgaaaacc
      421 gacatcgcca ttcgggtccg cgctaccgat cagattccgg tcattggccc aaggcgggat
      481 gctgccttgt aaggtcgtgc gggaggtcgg agttgcggcg ctggcgacca gtacggcggc
      541 caacaacatt ggaaagcctg cggctcggaa cagtttcgca ttcattatgt tatctccccg
      601 ccaggatcgt aacgcgaggc gccggcaaac gacgtcgctg ccgatgcgct tgaatcaacg
      661 tggtcaccgt cttgaggtcc atctcgtggt ggctctgctc acctttttat tccggcgcac
      721 attttgccgt ttgtgagaca tatagtccca tatgtggcgg aagtctagac ctataagctt
      781 gaggagccaa gcgtggcggg taaacccttc tcaacgagaa cggcatcgga gcctcgattc
      841 ggtttgcccc tcgtcgctgg tactggagat tttctcgcgc acctatatcg agcagctctc
      901 gctactgaaa gtacgtgttg gtcatctccc gcagcttgaa agatgtttgc tgacttcccg
      961 taaaaatcag aaagatcatt ccatggcaca tggcatagac tcggccctgg aatgacagga
     1021 gggcgagcct gttcccggct catttgcggt gtccccctat ccaacagcgg tctcaagaat
     1081 tcttgagagg actcaactat ccgccgcgtt ccgccctcgc tacaatccaa cgcacctgtt
     1141 cttacagaca taacaaaccg ttggggtagc gtgatcgcat gacgaagcac ctgagagcct
     1201 tgcgctttgc attctgcctc gcattgacag ttgcatccgg gcaggccgca ctcgctgccg
     1261 atcccccggc gcaaccgcag gacgctcagt acaccaagct gatcggcgac tatctcgtcg
     1321 ataaacgcat taccaccgag ttggtcgatc acatgccggc ctccgactcg gtgccttcgc
     1381 cgttgaagtt cttcggacgc attcccggca cgcccggtga gctgacgtac gcgaaggata
     1441 tcgagcgtta ctaccaggag ttggcgaaat catcgccccg ggcgcggttc tggaagctcg
     1501 gcacgtccga agagggccgt gaccaggtgg tgctcgctat cgcggacgag gagaccctca
     1561 aaaacctcga caagtacaag gccgacctgg cggcgctggc ggatccccgc cgcaccaccg
     1621 aggagcaggc aaaggccatc atccacacct cgaaacctgt ttactggatc accagcggca
     1681 ttcactcgcc cgataccggt ggcccggaaa tgctcatcga gctggccttc cgattgatgg
     1741 tcgaggaaac gccgttcatt cagaatatcc gcgacaacgt catcacattc atcacgccgg
     1801 tggtggaggt cgacgggcgc gagaaagagg tggataccta ctactacggt aagaagaccg
     1861 gcaagccgcg gccgccgctc atgtactggg gtaagtatgt cgcgcacgac aacaaccgtg
     1921 acggcatggg gcagttcctg aagctgacgc agaacatcac gaagggtgtg ctcgaatggc
     1981 atccgacagt gctccacgat ctgcacgagg cgcaatccta cttgtatgtg tcgacgggga
     2041 ccggcccgta caacccctcg ctcgacccgg tgcagtccga cgagtggtgg ctgctcgccg
     2101 agaccgaagt gtccgaaatg gcgaagcgca aagtcccggg cgtgtggacc tggggcttct
     2161 acgacggctg ggttcccaac tacctgttct ggatcgccat cacccacaac agtttcggcc
     2221 gcttctacga agtgcagagc tatggtcccg acatccagaa agggctcaag ctcgctccca
     2281 cggtgaccag ccacgagtgg tatcgcccga atccgccgtt accctcgatc gactggggtc
     2341 cgcgcaataa caccaacatc caggagtcgg cgatcctgtt tgcgctgaac cgtgttgcga
     2401 aagaccacga cctgtacctg gagaatttct ggatcaagag caaacatgcg gtagaagcgg
     2461 gccgctcggg tgccgtcaac gcctgggtca ttcccgcggc tcagcgcgcc cccctcaatg
     2521 tggctgcgat gatcaatgac ctgcgccacc agggtgtcga ggtcaatacc gccagcgagg
     2581 cgttcaccgc cggcgcagtg agcgtgtcga ccggggattt catcgtgcgc gcggaccagc
     2641 cgtaccggac tttggcggac atgtactttt cggtgcagga ctacccggcg ccgaatcccc
     2701 gaccgtacga cgacaccggt tggacgatgc agtacatgcg cgacgtgaag ctctcaccgg
     2761 tgcacgatcc ggaaatcctg aacaagtcca tgagcttgct cgccgccgat gtgaccccga
     2821 agggcgcgat c
//
LOCUS       DATAQL010001378         4509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_902092, whole genome shotgun sequence.
ACCESSION   DATAQL010001378 DATAQL010000000
VERSION     DATAQL010001378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902092"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VHS97_23710"
     CDS             <1..1333
                     /locus_tag="VHS97_23710"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX3451281.1"
                     /translation="KLGVVVITTADCANGVARHVAETALRMMLSVRIGLPLATPATSQ
                     AISGQQASQLEGHYVHGDHSVDITQRGGKLFLSPFADGMTVEVRKRGDSFVVDDRLAS
                     CSPIELDGNRIALEGKRYEKKATAKAAPLPADWEVLIGEYGWDHDVLYIFEKDGNLHT
                     LIEWFFDYPLKEIGPDQFLFPDYGLYAGERLVFTRDANQAVNHVEVASMVFKRRRLDG
                     EGGKSFRITPRRSVDEIRTDARSARPPTEPGEFRQPDLVELISLDPTIRLDIRYATTN
                     NFLGIPLYTQPRAFMQRPAAEALLRVHRSLAKDGFGLLIHDAYRPWQVTKLFWEATPD
                     SGRIFVADPAKGSKHNRGAAVDLTLYDLATGKPVKMVGGYDEFSPRSYPDYPGGTALE
                     RWHRELLRRSMEAEGFTVYEFEWWHFDHRDWTKYPILNQAFEDLTVTGGGK"
     gene            complement(1375..1899)
                     /locus_tag="VHS97_23715"
     CDS             complement(1375..1899)
                     /locus_tag="VHS97_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451282.1"
                     /translation="MALTFTVGGARGTFEDRFADEVAEVLDHAFGAEGEWEESPTLHY
                     GELADTGWSELQRRAIDELGADTIPNLNALGEDGRGVYLPAQLQAVSFPLSVGGPLKC
                     ASLLGLRNELAELAERWELPLDDVGLQALLRVAQDPDDGWVADPPEVLTFARLALAAN
                     EAMRRDCPLWLVGR"
     gene            2404..>4509
                     /locus_tag="VHS97_23720"
     CDS             2404..>4509
                     /locus_tag="VHS97_23720"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX3451283.1"
                     /translation="MPGSREGQLRAEVHALAVPTPENARSWLRTLTAEPHVAGTPADH
                     KTAVFVRDKLRDWGWKAELEELEVLLNYPRELPSLQITRPFNKKLSLDEAPLATDKDS
                     ASSRAFGAFNGYGVSGVVNGQVVYVNYGRPEDYVAIEKLGVDVKGKIVLARYGSLFRG
                     LKVRNAQKRGAAGILIFSDPADDGFAKGDVYPSGRFRPGSAIQRGSVQFLSLGPGDPS
                     TPFGPSVKGAKRLPFDVSDGFSMAELSRSGDDLIRRGGGGDQAAVLSREEWEKATGLK
                     RADYFATIPCLPISYDTAHEILKVLAGPNVPSGWQGGLPLAYHVGPGPAEVGMSVSMD
                     YQVRTIWNVIATITGAVEPDRWIMIGNHRDAWVYGAVDPGSGTAATLEMCRALGAAVK
                     NGWKPRRTLMYASWDAEEYGLVGSTEWAEQNASMLSEKAVLMLNVDSAVSGTEFGATG
                     VPSLRELVLDAAGAVINPRDGKSLRTAWTESHREAWAGSSPLVLADPIWDSEVAGTAH
                     SETAGQGKSGFFPQMASLGSGSDFTAFLDHLAIPAVEVGFSGGYGVYHSIYDSFNWME
                     KFGDPEFLTHAMAARLYTVIAMRAAAADVVPIKFVPYGLALREHVDQLRLIHVRRTRK
                     AEPGKMKARPEFEGLSSLVEAVCAFQVQADELDRATNAVTERGQFSGDELAGLNHALA
                     QVERAFLLDKGLPDRPWFKH"
BASE COUNT          860 a         1318 c         1442 g          889 t
ORIGIN      
        1 caagctgggc gtggtcgtaa tcacgactgc ggactgcgcc aacggagtgg cccgtcacgt
       61 cgcagagacc gcgctgcgga tgatgttgtc cgtccgcatc ggcctgccgc tcgcgacgcc
      121 ggcgacctct caggcgatct cgggacagca agcaagtcaa ctggaggggc attatgtcca
      181 cggcgaccat tcggtcgaca tcacacagcg gggaggcaag ctgtttctga gtccgtttgc
      241 ggacggcatg accgtcgaag tccggaaacg gggcgattcg tttgtggtcg atgatcgact
      301 tgcgtcttgt tccccgatcg agctcgatgg caatcgaatc gcgctggagg gtaaacggta
      361 cgagaagaag gctaccgcta aggcagcgcc tctgccagcc gactgggagg ttttgatcgg
      421 ggaatatggc tgggatcatg acgtgctgta cattttcgag aaagatggga acctgcacac
      481 gctcatcgag tggttctttg attatccgct gaaggaaatt gggccggacc aatttctgtt
      541 ccctgactac gggttatacg ctggcgagcg attggtcttc acacgagatg caaaccaagc
      601 cgtcaatcat gtcgaggtgg cgagtatggt cttcaagcgc cgccggctcg acggcgaagg
      661 gggcaagtcg tttcgcatca cgccgcgacg atccgtcgat gagattcgca ccgacgcgcg
      721 atcggctcgg ccgccgaccg agcccggaga gtttcgccaa ccggatttgg tggagctgat
      781 cagcctggac ccaacgatcc ggctcgatat tcggtacgcg acgaccaaca actttctggg
      841 cattccactt tacacgcagc cccgcgcctt catgcagaga ccggccgcgg aagctctctt
      901 gcgcgtgcac agatcgctag ccaaggacgg attcgggctc ttgattcacg acgcctaccg
      961 gccatggcaa gtgaccaaac tgttctggga agccactccc gattccggcc ggatcttcgt
     1021 ggccgaccct gccaaagggt ccaagcacaa ccgcggcgca gccgtcgatc tgacgcttta
     1081 cgacctcgcg acgggcaagc cggtgaagat ggtcggcggt tacgacgagt tttcaccacg
     1141 atcgtatccc gattatccgg gcgggacggc gcttgagcgc tggcaccgcg agcttttgcg
     1201 gcggtcgatg gaggctgagg gctttacggt ctacgaattc gagtggtggc acttcgacca
     1261 tcgcgactgg acgaagtatc cgatcttgaa ccaagccttc gaagacctga cagttacggg
     1321 aggtggcaag tgatgatttt tgtccacccc tcgttggtag cggaatcaag ccacttaccg
     1381 gcccacgagc cagagcgggc aatcgcggcg catcgcctcg ttagcagcca gggcgagtcg
     1441 agcgaacgtg aggacttcgg gcggatcggc gacccagcca tcgtcggggt cttgggcgac
     1501 gcgcagcaag gcctggagac cgacgtcgtc cagcggcagc tcccaccgtt cggccagctc
     1561 ggcgagctcg ttgcgcagac cgagaaggct ggcacatttg agcggtccgc cgacggaaag
     1621 tgggaacgag acagcctgaa gctgcgccgg cagatagacg ccgcggccgt cttcgcccaa
     1681 agcgttcagg ttgggaattg tgtcggcacc cagttcgtcg atcgctcgcc tttggagttc
     1741 ggaccatcct gtgtcggcga gctcaccata atggagggta ggcgattctt cccattctcc
     1801 ctcggcgccg aaggcgtggt cgagaacctc ggccacttcg tcggcgaaac gatcctcgaa
     1861 cgtgcctcga gcgcccccga cggtgaacgt tagagccatg gtgtggtcct tctcaaatca
     1921 ccagtggaag taagtctccg gttgtcacga gcgtaacgtg cgaggctctc agatgcagag
     1981 tggtagtaga agcagaattg gcttgccgga aagaatccag cgtcgaacga gtcgccggcg
     2041 gatgactgac atgcctctat caaggccgcc cgaagcatcg tatcttggat gcggcttttt
     2101 tcccgtgatc gggcccactc cgtccggcct ttgcctgccg ccagccactc cgcttcgtct
     2161 gtcccggccg aacttgcaga caactcccct accctgagtg tgctcctgag atttggtcct
     2221 gtcaagcagc ttatacttga gaaaggtctg ttgatgaggc gactctgcaa aaccctcggc
     2281 agtttgttgg cggtctgttt gttcataact ctgggcggcg gctcatttcg agggctcgaa
     2341 acgcttgcgt gcgcggcccc cggttcatcg gtcgacgcac ctgcggcatc accgctgggt
     2401 ttcatgccgg gttctcgcga ggggcaactc agggccgagg ttcatgcgct cgcggtgccc
     2461 acgcccgaga acgcgcggag ttggttgcgc actttaaccg ccgagcccca tgtcgccggc
     2521 acgcccgccg accacaagac cgctgtcttt gtgcgcgata aactgcggga ctggggttgg
     2581 aaggctgaac tagaggaact ggaggttttg ctcaattatc cgcgtgaatt accttctctt
     2641 caaattacgc ggcccttcaa caagaagctc tctctcgatg aggccccact ggcgaccgac
     2701 aaggattcgg ccagcagccg cgcgtttggc gcgttcaatg gctacggggt ctcaggcgtg
     2761 gttaacggac aagtcgtcta cgtaaactac ggccggcccg aagactacgt ggcgatcgag
     2821 aagctgggtg tcgatgtgaa gggcaagatt gtcttggccc gctacggcag tcttttccgc
     2881 gggctcaagg tgcgaaatgc ccagaaacgc ggcgctgccg gcatcctaat cttctcagac
     2941 ccggctgatg atggattcgc caagggagac gtctacccca gtgggcgatt tcgacctgga
     3001 tccgcaattc agcgcggcag cgtccagttc ctgtcgctgg ggcctggaga tccgtcgaca
     3061 ccgttcggtc cttcggtgaa gggtgcgaag cggctgcctt tcgacgtgtc cgacggtttt
     3121 agtatggcgg aactctctcg gtctggagac gaccttatcc gaaggggcgg cggtggcgac
     3181 caggccgctg ttttgtcacg ggaagagtgg gaaaaggcga caggtctcaa gcgagccgac
     3241 tactttgcga cgatcccctg cctgcccatc agctacgaca cggctcacga aatcttgaag
     3301 gttctcgccg ggcccaacgt accatccggc tggcaagggg ggctgccttt ggcttatcac
     3361 gtcggtcccg gaccggcgga ggtcgggatg tcggtgtcga tggattatca ggttcgcacg
     3421 atctggaacg tgatcgccac gattaccggc gcggtcgagc cggatcgctg gatcatgatc
     3481 ggcaaccatc gcgacgcctg ggtgtacggc gctgtcgatc cggggagtgg cactgcggcg
     3541 acgttggaaa tgtgccgagc gctcggggcg gcggtcaaga atgggtggaa gccgcggcgg
     3601 actctcatgt acgcaagctg ggacgccgag gaatacggac tggttggctc aactgaatgg
     3661 gccgaacaga acgcttcgat gttgagcgaa aaggccgtgc tcatgctcaa tgtcgattcg
     3721 gcggtgagcg ggacggagtt cggggcgacc ggcgtaccgt cgttgcgaga gttggtgcta
     3781 gacgccgccg gcgcggtcat caacccgcgc gatggcaagt ccctccggac cgcctggacc
     3841 gaatcccaca gagaggcatg ggccggcagc tcaccgctgg tccttgccga cccaatttgg
     3901 gactcagaag tcgcgggcac tgcccattcc gagactgccg gacagggcaa gagcggcttc
     3961 tttccccaga tggcctcgct ggggtcggga tccgatttca ccgcattcct cgatcatctg
     4021 gcaatcccgg cggtagaggt cggtttcagc ggcgggtacg gagtgtatca ctcgatctac
     4081 gacagcttca actggatgga gaagttcggc gatcctgagt tcttaacgca cgcaatggcg
     4141 gcccggttgt acacggtaat cgcgatgcgg gctgcggccg ccgacgtggt tccaatcaag
     4201 ttcgtcccct acggcctagc cctgcgtgaa cacgtcgacc agctcaggct cattcatgtc
     4261 cggcgaacgc gtaaggctga accgggcaag atgaaggcca ggcccgagtt tgaagggctg
     4321 tcaagcctgg tcgaagcggt gtgtgccttc caagtccagg cggacgagct cgatcgggcg
     4381 accaatgcgg tcacagagcg cggccaattc tccggcgacg agctggccgg gctcaaccat
     4441 gcgctcgcgc aggtcgagcg agcgttcctc ttggacaagg gcttgccgga tcgcccatgg
     4501 ttcaagcat
//
LOCUS       DATAQL010001379         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4873144, whole genome shotgun sequence.
ACCESSION   DATAQL010001379 DATAQL010000000
VERSION     DATAQL010001379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4873144"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VHS97_23725"
     CDS             <1..327
                     /locus_tag="VHS97_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX3451284.1"
                     /translation="IEGCKCGGSAQRRLRHWFMVHCSILHDFPIDRIVRYLTIPLRQP
                     AYRQGRDHRDFLRNLSLPRNILVDAIRGDCLAGSPSQPGLSVPWGVVQTLLSEKFANQ
                     AWIERF"
     gene            472..2235
                     /locus_tag="VHS97_23730"
     CDS             472..2235
                     /locus_tag="VHS97_23730"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3451285.1"
                     /translation="MERPTVANQSNLSLIEDYYQRWLNDPDSVDASWRNFFEGYDLGR
                     ESSDGSAPSGDAIAAQGQEAVTRLIDAYRAFGHYLADVDPLKLTKGRESPEHLEPAAF
                     GLNETDMDRVFYHKLSPSGHSTLRDLIQILRDTYCRTVGVEFMHIRDAEIRNWLLERM
                     EPVRNRPAFDIKQKRRIVYKLNVAELFETFLHKNYVGQKRFSLEGGEMLIPLLDTVIE
                     RAGGHGVKEIVMGMPHRGRLNVLTNILDKPYGMIFNEFEGNHLPETVGGDGDVKYHLG
                     FSADRVTADKHPIHLTLTANPSHLEAVNPVVEGRMRAKQRRFNDRDRRLGVPVLIHGD
                     AAFAGQGLVAETLNLSQLPGYRTGGTIHIVVNNQIGFTTSPSDGRSTRYCTDVAKMIE
                     VPIFHVNGEDPQAVIFVGELALDFRQRFGKDVVIDMVCFRRHGHNEGDEPAFTQPLMY
                     EKIKNRISIRELYTEQLVMAGDLTSQEAETIAETFADRLREVLEEVRRGGGQSPSDPR
                     GFAGAWKGLTPDFSFKPVETGVAYETLRRITEKAAQVPPGFTVNPKLARLIAGRVKTM
                     EAKGALDWANAEMLAFGSLLS"
     gene            2251..3279
                     /locus_tag="VHS97_23735"
     CDS             2251..3279
                     /locus_tag="VHS97_23735"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451286.1"
                     /translation="MSGQDSRRGTFSQRHAVLFDMRTGEAYTPLDHLKDNQPEFCVYD
                     SLLSEAAVLGFDYGYSLDEPHMLIMWEAQFGDFANGAQVIIDQFIASAESKWGRGSGL
                     VMLLPHGYEGQGPEHSSARLERFLQLSAEDNIQVAVPTTPAQYFHLLRRQVRRDFRKP
                     LIVMTPKSLLRHKLAVSPVDHLTVGHFHDVLDDPSSPDRVRRVLFCSGKVYYDLVAKR
                     AEVGSQREVAVIRLEQLYPWPVDQLKSVLERYRSAREWIWVQEESQNMGAWTFVAPRL
                     QELMGLSVQYVGRDASASPATGSKLVHDREQAEIVEAAVGGAAVPHLVSASSIRQRAA
                     LTARPGVS"
     gene            3282..>4002
                     /locus_tag="VHS97_23740"
     CDS             3282..>4002
                     /locus_tag="VHS97_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX3451287.1"
                     /translation="MPSVPVKVPSVGESITEGILAHWMKADGEAVLEGDSLYELETDK
                     ASSVFPAPSSGVLKIGVAEGDTVAIGATIGTIDPAGTPKAATPKAPAAAPAAPTPSAG
                     RANTPAAPAANEQEKRPAAPAAPATAAPGDGSSSQRLSPAVRRIVAEAGVDVARVAPT
                     GPGGRVTKRDVLAYLDEPQPTADTTSTSAPAAAYAPAPANSSRTAERETRQRMSGLRQ
                     RIAQRLVEVQQTAAILTTFNEA"
BASE COUNT          788 a         1242 c         1262 g          710 t
ORIGIN      
        1 atcgaggggt gcaaatgcgg cggcagcgcc cagcggcggc tgcggcactg gttcatggtt
       61 cactgttcga tcctgcacga ctttcccatc gaccggatcg tgcgttacct gacgattcca
      121 cttcgccagc cggcatatcg ccaggggcgc gaccatcgcg acttcttgag gaacctcagt
      181 ttaccgcgta atattctggt ggatgcgatc aggggagatt gtctcgccgg ctcgccatcg
      241 caaccggggc tgagcgtgcc gtggggagta gtccaaacgc tgttgtcgga gaaattcgcc
      301 aatcaggcct ggatcgagcg attctagttc gccattctcc gggcgccgtg acgtatgata
      361 agtccaacgg tctcgtcgct cggcgagcga cgggagtctt caaccgggaa ccgaccacac
      421 ggcctgcgcc ggcccccttt gcacatccga ctggcctgga accacgctac catggaacgc
      481 ccgacagtcg ccaaccaatc gaacttgagc ctgatcgagg actactacca gcgctggctc
      541 aacgaccctg actcggtcga cgcatcttgg cgtaactttt ttgaaggata tgatttaggc
      601 cgcgaatcct ccgacggctc cgccccgagc ggcgatgcca tcgcggccca ggggcaagag
      661 gccgtcacgc gtctcatcga cgcttatcgc gcgtttggcc actatttggc cgacgttgat
      721 ccgctcaagc tcaccaaagg gcgcgagtct cccgaacatc tggagccggc agcgtttggc
      781 ctgaacgaaa ccgacatgga ccgggtcttc taccacaagt tgagcccgag cggtcattcg
      841 accttacgcg acctgatcca gatcttgcgc gacacctatt gccgcacggt cggcgtcgag
      901 ttcatgcata tccgtgacgc cgagatccgc aactggctgc tcgagcggat ggagcccgtg
      961 cgcaaccggc cggccttcga tatcaagcaa aagcgaagga tcgtctacaa gctcaacgtg
     1021 gccgagctgt tcgagacgtt tctccacaaa aactatgtgg ggcaaaagcg gttttcgctc
     1081 gaagggggcg agatgttgat tccgcttttg gacacggtca tcgagcgggc gggaggacac
     1141 ggagtcaagg agatcgtcat gggcatgccc caccgcgggc ggttgaatgt cctgacgaac
     1201 attctcgaca agccgtacgg catgattttt aacgagttcg agggaaacca tttgccggag
     1261 acggtcggcg gcgacggcga tgtgaagtat cacctcggtt tctcggccga tcgcgtcacg
     1321 gccgacaagc acccgatcca cctgacgctg acggccaatc ccagccactt ggaggcggtc
     1381 aacccggttg tcgaggggcg gatgcgggca aagcagcgcc gattcaacga ccgcgaccgc
     1441 cggctgggag tgcccgtctt gattcatggc gacgcggcgt tcgccggaca ggggctggtc
     1501 gcggaaacct tgaacctctc gcagctcccc ggttatcgaa ccgggggtac aatccatatc
     1561 gtggtcaata accagatcgg tttcaccacg tcccccagcg acgggcggtc gacgcgctac
     1621 tgcaccgacg tcgccaagat gatcgaggtg ccgatcttcc acgtcaacgg cgaagatccg
     1681 caagccgtga tctttgtcgg cgagctggcg ctcgacttcc gccagcgctt cggcaaagac
     1741 gtggtcatcg acatggtctg ctttcggcgg cacgggcaca acgaggggga cgagcctgcg
     1801 tttacccagc cgctcatgta tgaaaagatc aagaatcgta tcagcattcg cgagctgtac
     1861 accgagcaac tggtgatggc gggcgatctg accagccagg aggccgagac gatcgccgag
     1921 acctttgccg acaggctgcg cgaggtgctc gaggaggtcc ggcgcggcgg cggccagtcg
     1981 ccatccgacc ccagaggctt cgcgggcgca tggaaggggc tgacgcccga tttttcgttc
     2041 aagccggtcg agacgggggt cgcgtacgag acgctacgca ggatcacgga aaaggcggcc
     2101 caggtgcccc ctgggttcac ggtcaatcct aaactggcca ggctcattgc ggggcgggtc
     2161 aagaccatgg aggccaaggg agctctcgac tgggccaatg ccgagatgct cgcttttggc
     2221 tcgttgttga gctaagacac cccagtccgc ttgagcggcc aggatagccg ccgcggcaca
     2281 tttagccagc ggcacgccgt gctcttcgat atgaggacag gcgaggccta tacgcccctt
     2341 gatcacttga aagacaacca gcccgaattt tgcgtttatg acagcctgct ttccgaggcg
     2401 gccgtgctgg ggtttgacta tggctactcg cttgacgagc cgcacatgct gatcatgtgg
     2461 gaggcccagt tcggcgattt cgccaacggc gcgcaggtca tcatcgacca gttcattgcc
     2521 tccgccgagt cgaaatgggg gcgcggcagc ggcctggtca tgctcttacc gcacggctac
     2581 gagggacaag gccccgaaca ttcgagtgcg cggctggaac ggtttctcca gctctctgcc
     2641 gaagacaaca tccaggtcgc cgtgccgacc acgccggcgc agtactttca cctgttgcgc
     2701 cgccaggtgc ggcgagactt tcgcaagccc ttgatcgtga tgacgcccaa gagcctgctg
     2761 cggcacaagc tcgcggtatc gccggtcgat cacctgactg tgggccattt tcatgacgtc
     2821 ctcgatgacc caagctcccc tgaccgggtg cgccgggtct tattctgctc gggaaaggtc
     2881 tattacgacc tggtcgccaa gcgggctgag gtcggcagtc aacgcgaagt cgccgtgatt
     2941 cgactcgagc aactgtatcc ctggccggtc gaccagttga aatcggtgct cgagcggtac
     3001 cgatccgcac gcgagtggat ctgggtgcag gaagagtcgc aaaatatggg agcgtggacc
     3061 ttcgtggcgc cacggctgca agaactcatg ggattgagcg tccagtacgt cgggcgggac
     3121 gccagcgcca gtccagccac cggctcgaag ctggtccacg atcgggaaca ggctgagata
     3181 gtcgaggctg ccgtcggcgg cgcggccgta cctcacctgg tttccgcttc gtcgatccgc
     3241 cagcgcgcgg ccctgacagc ccggccagga gtaagctgaa catgccgagc gttccagtca
     3301 aagtgccgag cgtgggagaa tccattacag aagggattct ggcacattgg atgaaggctg
     3361 acggtgaagc ggttctagag ggggattcgc tctacgagct cgagaccgat aaggccagca
     3421 gcgtctttcc ggcgccgagc tcgggggttc tcaagattgg cgtcgcggaa ggcgataccg
     3481 tcgccatcgg cgcgaccatc ggcacgatcg acccggccgg aacgcctaaa gcggccactc
     3541 caaaagcccc tgcggcagcg cctgcagcac cgacgccctc ggcgggccga gcaaatacac
     3601 ccgctgcgcc ggccgcaaat gagcaagaaa aacgaccagc ggcaccggcc gcaccggcta
     3661 cggctgcgcc tggtgacggg tcaagtagcc agcggctttc gccggcggtg cggcggattg
     3721 tcgcggaggc tggggtagac gtggcgcgcg ttgctcccac cggacccggt ggccgcgtga
     3781 cgaaacgtga tgtgttggcc tacctcgatg aaccacaacc gacggcggat acgacatcga
     3841 catcggcccc agccgctgcc tacgcaccgg caccggccaa ttcaagccgc acggccgagc
     3901 gcgaaacccg ccagcggatg agcgggctgc gccagaggat cgcccagcgc ctggtcgagg
     3961 ttcagcaaac cgccgcgatc cttaccacgt tcaacgaagc cg
//
LOCUS       DATAQL010001380         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2582820, whole genome shotgun sequence.
ACCESSION   DATAQL010001380 DATAQL010000000
VERSION     DATAQL010001380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582820"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1039)
                     /locus_tag="VHS97_23745"
     CDS             complement(23..1039)
                     /locus_tag="VHS97_23745"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX3451288.1"
                     /translation="MDETGPYQVVENWFKPGVDHWNQVVTSVAVDTPNRIIIASADEH
                     MARPASLILSADGVPYNLRPDAKETPKPEAEKTHLHQILVLNAAGKVVEDWSQWNDLI
                     VVPHSVAINPYDKERHVWIIDREGQQILEFTNDGKKLVLKIGEKGVAGTDQGHFNLPA
                     GIAFLPDGSFYVADGYRNTRVIKFDAHGKYQFEWGSKGAEPGQFNLVHAVAVDRLKRV
                     FVADRSNNRIQIFDAQGKFLDQWTNIRQPAFIQISADNKSVWVASNGEDRIAKYDMNG
                     VLQTYWGVHGRFPGWIDDFHHFAVDPAGNLFVADAFNNRVQKFVPRAGADKARVVDQP
                     FVFK"
     gene            complement(1166..>1539)
                     /locus_tag="VHS97_23750"
     CDS             complement(1166..>1539)
                     /locus_tag="VHS97_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451289.1"
                     /translation="ALSICTGILVKQAKLRYAWVTGAPLVWLAIVTTAATWEKVVSSD
                     VRVGFLAAADHMAANLAAGTLPAAQAAVAPQLIFNQRLDAVLSVVLAAILWVVIADTV
                     RVCLEVIRGMPVRPSSEAGAV"
BASE COUNT          296 a          496 c          437 g          310 t
ORIGIN      
        1 ccaacaccca acctggctgc tgttacttaa atacaaacgg ctgatccacc accctcgctt
       61 tatcggcacc agcccgcggc acaaacttct gcacccggtt gttgaacgca tccgccacga
      121 aaagattccc cgccgggtcc acagcgaagt ggtgaaagtc gtcgatccac ccggggaagc
      181 ggccatggac gccccaatag gtctgcaaca ccccattcat atcatacttc gcgatacggt
      241 cctcgccgtt cgaggcgacc cacacggact tattgtccgc cgagatctgt ataaaagcgg
      301 gctggcgaat attcgtccac tggtcgagga attttccctg cgcatcgaag atctggatgc
      361 ggttgttgct ccgatcggcg acgaaaaccc gcttgaggcg atccacggcg accgcgtgca
      421 ccaggttgaa ttggccgggc tccgcgccct tgctacccca ttcgaactgg tacttgccat
      481 gggcgtcgaa tttgatgacc cgggtattcc ggtagccgtc cgcgacgtaa aacgagccgt
      541 cgggaaggaa ggctatgccg gcgggtaggt tgaaatgccc ttgatccgtc cccgccacgc
      601 ctttctcgcc gatcttcagg accagtttct tgccgtcgtt ggtgaattca agaatctgct
      661 ggccttcacg atcgatgatc caaacgtgcc gctccttatc gtagggattg atggcaacgc
      721 tgtggggaac cacgatcaga tcattccact ggctccagtc ttcgacgact ttgccagcgg
      781 cattcagcac caggatctgg tgtagatgcg tcttttccgc ttcgggtttc ggagtctcct
      841 tggcatcggg cctcaggttg tacgggacgc cgtcggcgct gaggatcagc gacgccgggc
      901 gcgccatgtg ctcatccgcg ctggcgatga tgatgcggtt tggcgtgtcg acggcgacgg
      961 aggtgacgac ctggttccaa tggtcgacgc ccggcttgaa ccagttctcg acgacctggt
     1021 acgggccggt ttcgtccatt ccgccgcgct ccgtcgtctt ttgcgcgagg gccgggccgc
     1081 tcgccacggc gcaagttgca agggctgccc acgtggcgag cctgactacc ttgagaatct
     1141 tcatcgacgt cttcccccta gttgactaaa ccgcccccgc ctcagaactg ggccgcaccg
     1201 gcatgcctcg aatcacctcc aggcaaacgc ggacagtatc cgcgatcacc acccaaagta
     1261 tggcggccaa caccacggac agcaccgcgt ccagacgttg attgaaaatc aactgcggcg
     1321 ccacggctgc ctgcgcggcc ggcagcgtac cggctgccag atttgccgcc atgtggtcgg
     1381 ccgcggcgag aaatcccaca cgcacgtcgc tggagacgac cttctcccag gtggccgccg
     1441 tggtaacgat tgccagccag accaacggcg cgccggttac ccatgcgtaa cgcaattttg
     1501 cctgcttcac caggatgccg gtgcagatgc tcaaagcga
//
LOCUS       DATAQL010001381         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1972891, whole genome shotgun sequence.
ACCESSION   DATAQL010001381 DATAQL010000000
VERSION     DATAQL010001381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972891"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VHS97_23755"
     CDS             complement(<1..969)
                     /locus_tag="VHS97_23755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3451290.1"
                     /translation="MKGKVMRDSRLGPVLKEIHALYSVGTQTGLTDGELLERFVTNEG
                     EGSQFAFSALVRRHGPMVLRVCQRLLSDVHESQDAFQATFLILAKKAGSLRDCGSVGP
                     WLFGVANRVAAKSRTATERRTRHERQYLERLATEGQIEDKDRWDVNVVLHDEVRRLPD
                     RFRIPIVLCYLEGVTQEEAAAQLGWPVGTVASRLARARERLRGRLTRRGVTLSATALA
                     SALAQNVSAAPSMLVNSTVRAAISFAEGGATTVWVVPASVDLLMKTVARSILMTKLKI
                     AGAVLITVGALATSAGVFAYQPAGPDEFEPPAQTEWGLEKPERESRS"
BASE COUNT          325 a          555 c          470 g          325 t
ORIGIN      
        1 ggaccgggac tccctctctg gtttctcaag gccccactct gtctgggccg gcggttcgaa
       61 ctcgtccggc cccgctggct gataggcgaa aacacccgcg cttgtcgcca gtgcacccac
      121 tgtaatcaga accgcccctg cgatcttcaa cttggtcatt agaatgctcc tggctacggt
      181 tttcatcaac aaatcgacag atgccgggac cacccaaacg gtcgtcgctc caccttccgc
      241 gaaactgatt gcggctctca ccgtggaatt gacgagcatc gaaggcgccg ccgacacgtt
      301 ttgcgcaagc gcgcttgcta gcgccgtcgc tgaaagggtc accccgcgcc gggttagtcg
      361 gccccgaagc cgttcccttg cccgtgcgag acggctcgcg accgttccga ccggccagcc
      421 aagctgggcc gcggcttcct cctgcgtcac accttcgagg taacagagta cgatggggat
      481 ccggaaccga tccggaagcc gtcgaacctc atcgtgcaat acgacgttga cgtcccatcg
      541 gtccttgtcc tcaatctggc cttcagtcgc cagtcgttcg agatactggc gctcgtgtcg
      601 tgtgcgccgc tccgtggcgg ttctcgactt ggcagcgacc cggttggcca cgccgaataa
      661 ccaagggccg accgagccgc agtctctgag cgaaccagct ttcttagcca gaatcaagaa
      721 agtcgcctgg aatgcgtcct gactctcgtg cacatcagat agaagacgct gacagacccg
      781 gaggaccatt ggaccgtgcc gtctcaccag cgcggagaac gcgaactgcg agccttctcc
      841 ctcgttggtg acaaaccgct ccagaagctc accatccgtc agaccggttt gcgttcccac
      901 gctgtagagc gcgtggattt ctttcagtac cggacccaga cgactgtccc gcatgacctt
      961 gcctttcaac cccagaatcc gcctagtact ccctctccgc ggctcgatct atccgttttt
     1021 cttggaaaaa gccgaagcag aaaggcattg caccgcctgc cgttgaccgg aggacggatg
     1081 agcccgggta tgcaacccgt tagtgcagag cagtttcgga tcactgcgcg atgcccagac
     1141 cgagggattc cccgatcgcg cgagagacca tccacgcttc gacgtgcgac gtccctccgc
     1201 ggccgagcct gccgcttctt tcccgtcccg tgccgaaagt ggtgaggaag ttcccaaccg
     1261 tattgcgttg acgaacgacg acgccgtccg ccgttgctat cgcaaatctg gcctcgcgcg
     1321 cagcccatga gctcgaagtc tttcccgcgc caatcggggt cgaacgcggg cccagacgat
     1381 tctggcaaac gatgatgctg gcccttgaat tgcaacatgc aacccttttt cgggatgaac
     1441 ccatggctgt ggccctcgca ctgaacaagg aaaaaatcgc tcgaagctca atgccgtcac
     1501 gcacggcatg cgggctgaaa cactcgtgtt gatcgatgag gatcctcagg cgctcgaaga
     1561 ccggagagcg gcctcgaacg cccggctggt gccgtctgat gacgtcgcgc gacgcattgt
     1621 cgaagacgcc gtcgtgtcga catggctgca agatcgggcc cggcgcgctc aggtc
//
LOCUS       DATAQL010001382         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1211061, whole genome shotgun sequence.
ACCESSION   DATAQL010001382 DATAQL010000000
VERSION     DATAQL010001382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211061"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1567
                     /locus_tag="VHS97_23760"
     CDS             <1..>1567
                     /locus_tag="VHS97_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEX3451291.1"
                     /translation="ASLDVVSRAWRRFVIGPDRTIDRRAYTLCVLQRLQDGLRRRDVF
                     VERSERWGDPRAKLLRGTEWEAARSQVCLSLGRRATAAAELESLRRQLDDAYRRTADN
                     LPANEAIRIEVVAGRDSLTLTPLDKLDEPASLWTLRQAVADLLPRVDLPDVLLEIQAR
                     TNFAEAFTHVSEAEARVTDLAVSVCAVLLAEACNIGLEPLIRPEVSALTRGRLAWVQQ
                     NYIRAETLIRANALLVDAQTKVPLVGAWGGGEVASADGLRFVVPVRTVNAGPNSKYFG
                     AGRGITYYNFTSDQFTGFHAIVIPGTLRDSLFILEGLLEQQTSLRPLEIMSDTAGASD
                     VVFGLFWLLGYQFSPRLADVGEARFWRMDPAANHGAMNGLARHRVNTELIARNWDDML
                     RVAGSLARGTISASELIGSLLRSSRPSTLTRAIGELGRVAKTLYLLSYLDDETYRRRI
                     LTQLNRGESRHGVARAIFHGQRGEVRQRYREGQEDQLGALGLVVNVVVLWNTLYMDAA
                     LAHLRCQHVETNPE"
BASE COUNT          258 a          528 c          512 g          269 t
ORIGIN      
        1 agcctccttg gacgtggttt cgcgcgcctg gcggcgattc gtgatcggtc cggaccggac
       61 gatcgaccgc cgcgcctaca ctctatgcgt gctgcagcgg ctccaggacg gcctccgccg
      121 tcgcgacgtg ttcgtcgaac gtagcgaacg ctggggcgac cctcgagcta agctcttgcg
      181 ggggacggag tgggaagcgg cccggtccca ggtctgtctc agtttgggtc gccgggcgac
      241 cgccgccgct gaactggaat cactccgccg ccagctcgac gacgcctatc gacggaccgc
      301 cgacaatctc ccggccaacg aggccatccg catcgaggtc gtcgcgggcc gcgactcgct
      361 gacactgact ccgttggaca aactggacga acccgctagc ctgtggacgc tccgtcaggc
      421 agtggccgat ttgctcccgc gtgtggacct gccggacgtc ctcctggaaa tccaggccag
      481 gaccaacttc gccgaggcgt tcacccatgt cagcgaggcc gaggcccgcg tgaccgacct
      541 ggcggtcagc gtttgcgcgg tgctgctggc cgaggcctgc aacatcgggt tggagccgct
      601 gatccgcccc gaggtatccg ccctgacccg cggtcgactc gcctgggtcc agcaaaatta
      661 catccgggcc gagaccttga tccgcgccaa cgccctcctg gtcgatgccc aaaccaaagt
      721 cccgctggtg ggggcctggg gcgggggaga ggtcgcctca gccgacggcc tgcgtttcgt
      781 ggtaccggtc cgcaccgtca acgccggacc caacagcaag tacttcgggg ccggccgagg
      841 gatcacctat tacaacttca cctccgacca gttcaccggg ttccacgcca tcgtgatccc
      901 cggcacgctc cgcgactctc tgttcattct ggaaggcttg ttggaacaac agacgagctt
      961 gcgtcctctg gaaatcatga gcgatacggc aggggccagc gacgtggtct tcggactgtt
     1021 ttggcttctg ggctaccagt ttagccccag gctggccgac gtcggcgagg cccggttctg
     1081 gcgcatggac cccgccgcca accacggggc gatgaacggt ctggcgcggc accgagtcaa
     1141 caccgagctg atcgcccgga actgggacga catgctacgg gtggcggggt cgttggctcg
     1201 ggggaccatc agcgcctcgg aattgatcgg ctcgctgctg cggagcagcc gtccctcgac
     1261 cctgacgcgg gcgatcggcg agttggggcg ggtggctaag acgctctacc tgctttccta
     1321 ccttgacgac gagacttatc gccggcgcat cctcacacag ctgaaccgtg gcgagagtcg
     1381 ccacggcgtg gcccgggcga tcttccatgg ccagcgcggc gaagtccgcc aacgctatcg
     1441 cgagggccag gaagaccaac tcggagcgtt gggactggtc gtcaacgtgg tggtgctctg
     1501 gaacaccctg tacatggacg cggcgctcgc ccatctgcgt tgccagcacg tcgagacgaa
     1561 tcccgag
//
LOCUS       DATAQL010001383         6001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1972904, whole genome shotgun sequence.
ACCESSION   DATAQL010001383 DATAQL010000000
VERSION     DATAQL010001383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972904"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VHS97_23765"
     CDS             complement(<1..824)
                     /locus_tag="VHS97_23765"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX3451292.1"
                     /translation="MTTRTALIFPEWKQETWDRLFGYRNAFDEPVVAVIVSILGVALT
                     ITPLAILIMDRAGRLGPALKDDLWRRYVSWLVMVPVVILPILLGAAWSLLLFTALGLL
                     SFREFARATGVFREKVMSLLVVIGILSLTFAVADHFYRLFVALTPMNIVVITAVATSQ
                     DRPKGYIQRVALTIFGFMLFGACLGHLSYMTNDTHYRSLVLLLIFCVQLNDVFAFIVG
                     KSLGGPKLAPLTSPNKTISGALGAVVLTTLLVYWLGGIVFPAGQLAEPLQRVVLGFL"
     gene            1152..2201
                     /locus_tag="VHS97_23770"
     CDS             1152..2201
                     /locus_tag="VHS97_23770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX3451293.1"
                     /translation="MREITADTAADYLRESGWVPDRAVIDVRELSGGVSNIVLRVDTQ
                     GKAPIVIKQCRERLRVAMDWRAPLDRIWTERATLDLLHSILPEGTVPTVLFEDRPEYL
                     FAMTCAPDDAVTWKADLMAGQIDPGIAARLGTILGTIHAKASSSPALSETLSDTSLFE
                     ELRVDPYYRTAARAHPNLASRIALLIDSMNRPAGERNLVLGDFSPKNILVHSGGLILL
                     DFECAHAGDAAFDLGFFLSHLLLKTIRAAAVDRQLASRYLALTPQFWAGYLDSRGFDT
                     VARSELVRRAVQHVAACALARVDGKSPVEYLDVAGQAVARSFALEVLLNGPTTWDDLC
                     MVLSNAIGGGIEWQV"
     gene            2189..3517
                     /gene="eno"
                     /locus_tag="VHS97_23775"
     CDS             2189..3517
                     /gene="eno"
                     /locus_tag="VHS97_23775"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX3451294.1"
                     /translation="MASLKALKARQVLDSRGRPTVEVDAIASTGALGRAIVPSGASTG
                     RHEALELRDPENPHFGGLSVTRAIDNVVHEIARAVLGRDLDDQTGLDAQLIALDGTLN
                     KSRLGANALLGVSLAVAHAAAAARGEELFVHLNRLWCNQLGGSGTASASSEPTLPLPM
                     VNMISGGLHAGGNLDVQDVLIIPVGASRYRDALEMIVAIYRSVGAVLKKQGFESDLVG
                     DEGGFGPRLRSNEQAFEIVVEAILACGFEPRRDVAIAVDVASTHFFDPVRGVYRLRST
                     GEQILDSQGVIDLLAGWIERYPIVSIEDGLAEDDWDGWAAITNRFDTSVQLIGDDLFA
                     TQVDRLQKGIDRHAGNAILIKLNQVGTLTETLGALKLAREHGFRAVVSARSGETEDTT
                     IADLAVATAAGQIKIGSVARSERLAKYNRLLRIEEALGQTGRFAGRSNLI"
     gene            complement(3620..4327)
                     /locus_tag="VHS97_23780"
     CDS             complement(3620..4327)
                     /locus_tag="VHS97_23780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451295.1"
                     /translation="MLQTDQGEQVPGAVGEHRAVDFGVVLNCDQKVVERFLGPALRQR
                     GERVLGGVHVLGSHPLAQGLAPHTPWGERHRCDRTQNLVASSTALLDAAGVAIKDFQD
                     GQRPRGLRQLAAHVKRRCHRHHRVKADIIFTAERPGVGKRSRRDQPLQFDPTLQFVDH
                     DGHETLRGRLLHDRDQAFQLPEVQTRLRTVDPRAGQSEVSGGARTERRDQSPSSHVRE
                     KLSSVSLGTHGDLLPSG"
     gene            complement(4349..4843)
                     /locus_tag="VHS97_23785"
     CDS             complement(4349..4843)
                     /locus_tag="VHS97_23785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451296.1"
                     /translation="MEKVAPSLHAVTSNLVGAEYQAGDGISRVAHLGHVSPRIGRVAR
                     VDSDRITVDAAGWLVGLSFGSAAAGVAQDQLVGRERDGFADRVRGHALGQGGRCRVGG
                     LIGGSLENGVTQVLDPRGVGLPSPERLSGQVILVQEFGQDDAGLVRLGRVLPVAFALD
                     AIVD"
     gene            complement(5291..5902)
                     /locus_tag="VHS97_23790"
     CDS             complement(5291..5902)
                     /locus_tag="VHS97_23790"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX3451297.1"
                     /translation="MDLIDYGADPKDDRKFRSHLGKLVTKTLNKIVAIPVVKDHGSAG
                     VTGALKNMSHGTVNNVNRSHSTPFTNVCNQFIPQMVSHPMIRQKFVLQIMDGIRGVYQ
                     GGPFARREGKWTWENNSIFFATDPVALDHVEWDIVDAQRLKQNLPPVAAAGGKAIDPL
                     KTEGFDVRQPQHIALAGALGLGYFDYKSPKGRRFSIQHRVVNV"
BASE COUNT         1193 a         1816 c         1856 g         1136 t
ORIGIN      
        1 agaaatccca gtaccacacg ttgcagcggt tcggcgagtt gacccgccgg gaacacgatt
       61 cccccaagcc agtacaccag aagggtcgtg agcacaacag cgcccagcgc gccggatatc
      121 gtcttgttcg ggctggtcag cggtgccagt tttggccctc ccagcgactt gcccacgata
      181 aaggcgaaca catcgttaag ctgcacgcaa aaaatcaaga gcaagacaag cgaacgatag
      241 tgcgtgtcgt tggtcatgta ggaaaggtgg cccaggcagg ctccgaacaa catgaagccg
      301 aagatcgtca gggcgacgcg ctggatatat cctttgggcc ggtcttgcga cgtggccacc
      361 gcagtgatca ctacgatgtt catgggagtc agcgcgacga atagacgata gaagtggtcg
      421 gccacggcaa aggtgagcga cagtatccca atcacgacga gaagactcat gaccttctcg
      481 cgaaacacgc ccgtcgcccg ggcgaactcc cggaagctaa gcaagccaag cgccgtgaac
      541 aacaggagtg accatgcggc ccccaacaag atgggcaaaa tgactacagg caccatgaca
      601 agccaagaaa cgtagcgccg ccagaggtcg tccttgagag ccggtccgag ccggcccgcc
      661 cgatccataa ttaggattgc caacggtgtg atggtcagag caactccgag gatcgatacg
      721 atgactgcca cgaccggctc gtcgaaggcg ttgcgatagc cgaaaagacg atcccaggtc
      781 tcctgcttcc actcggggaa gatcagagca gttctcgtcg tcacgacacc ttgacccttg
      841 gctgacaaaa actcaacgcg ctctcaagta gttcatggcg aaaaaatccg atggcgtgcc
      901 aacttcgcaa gacgattcat tcgatcgggc tggctccgct tgaaaggtcg acaacgacac
      961 gagcccgcaa gagatcatga agctacgagg accgttgccg aagatcaatc tgttgcattc
     1021 ctcgtttcga tacttctgga agttggtctg acatccgatg acccactcga ccggaagtgg
     1081 atgtggcgcg cagtctgaga aatccttgat cggcgattgc gtgcagcggt agagttcatg
     1141 aaacagagcc aatgcgtgaa atcaccgcgg acacagctgc cgactatctc cgtgaatccg
     1201 gttgggttcc cgatcgagct gtgatcgacg tccgcgagtt gagtggcggg gtttccaaca
     1261 tcgtcctgcg agtcgacact caaggaaaag cgccgatcgt gatcaagcaa tgccgtgagc
     1321 ggctacgcgt ggcgatggac tggcgcgcgc cgctcgaccg gatctggacg gaacgcgcca
     1381 cacttgatct actccattcg atcctgcctg aaggaacggt cccaacggta ttgttcgagg
     1441 atcgtcccga gtatctgttc gcgatgacct gcgcccctga cgacgcggtt acgtggaagg
     1501 ccgacttgat ggctggccag atcgatccgg gcatcgccgc gcggctgggt acgatcctgg
     1561 gtacgatcca tgccaaagcc tcttcctcgc ctgccctgag cgaaaccttg tccgacacga
     1621 gcctgttcga ggagctacgg gtcgacccct actaccgtac ggccgcgcgt gcccatccga
     1681 atctggcctc gcgcatcgca ctgttgatcg actccatgaa ccggcccgcc ggcgagcgga
     1741 acctcgtcct gggggacttt agcccgaaga acatcctggt gcactcgggt ggactgatcc
     1801 tgctcgattt tgagtgcgcg catgcgggcg acgcggcttt cgacctgggg tttttcctga
     1861 gtcacctcct gctcaagacg atcagagccg ctgccgtgga tcgacagctc gcctcgcgat
     1921 acctcgcgct gactcctcag ttctgggccg gctatttgga tagtagagga tttgacacgg
     1981 tcgcccggtc agagttggtt cgtcgtgccg tccagcacgt cgcggcttgc gcgctggctc
     2041 gggtcgacgg caagagcccg gtcgaatacc tcgacgtcgc gggtcaagcc gtcgcccggt
     2101 ccttcgccct tgaggtcctt ctcaatgggc cgaccacatg ggacgatctg tgcatggttc
     2161 tcagtaatgc gatcggtggg gggatcgaat ggcaagtttg aaggcactca aggcccgcca
     2221 ggtgctcgat tcgcgcggtc gtcctacggt ggaggtggat gcgatcgcat cgaccggggc
     2281 cctgggacga gcaatcgtac catcgggcgc cagtacgggc cggcacgagg cgctcgagct
     2341 tcgcgacccc gaaaaccctc attttggcgg tttgagtgtc actcgggcta tcgacaacgt
     2401 ggtccacgag atcgcccggg ccgttctcgg cagggacctg gacgaccaaa ctggcctcga
     2461 cgcccaactc atcgcgctcg acggtacttt gaataagagc cggctgggtg ccaacgcact
     2521 gctcggcgtt tcactggccg tcgcccatgc ggcggctgca gcacgcggtg aagaactctt
     2581 cgttcacttg aacaggcttt ggtgcaacca acttggcgga tcgggaaccg cgtccgcttc
     2641 gagcgagccg accttgccgc tcccgatggt gaatatgatt tcaggcggtt tgcatgccgg
     2701 cggcaacctc gacgttcaag atgtgctgat catcccagtc ggcgcgagca ggtatcgcga
     2761 tgctctcgag atgatcgtcg cgatctatcg ttctgtcggc gccgtgctaa agaaacaggg
     2821 ctttgagtca gacctggtgg gcgacgaggg gggcttcggg ccgcggctgc gaagcaacga
     2881 acaggcgttc gagatcgtcg tggaggccat actcgcctgc ggctttgagc ccaggcgtga
     2941 cgtagcgatc gctgtcgatg tggcttccac gcacttcttt gaccccgtcc gcggagttta
     3001 ccgacttcgc tcgacgggcg agcaaattct cgacagccag ggcgtgatcg acctcctggc
     3061 cggctggatc gagcggtatc cgatcgtttc gatcgaggat gggctcgccg aggacgactg
     3121 ggacgggtgg gccgctatca cgaaccgatt tgacacttca gtccagctca tcggcgacga
     3181 ccttttcgcc acgcaggttg accgcctcca aaagggcatc gaccgccatg ccggcaatgc
     3241 catattgatc aagctcaatc aagtgggaac gcttaccgag accttgggtg cgctcaagct
     3301 ggcgcgcgaa cacggctttc gcgcggtcgt ctcggcccgc tcgggcgaga ccgaggatac
     3361 aacgatcgcc gacctggccg ttgccaccgc ggccggtcag atcaagatcg gttcggtcgc
     3421 gcggtcggaa cggttggcca agtacaatcg attgctacga atcgaagaag cccttggcca
     3481 aacgggccgt ttcgccggac ggtcgaattt gatctgactg gctcgtctct tgcttcttga
     3541 ccggtctcgg atcaacaatt gctgaaagct cctgaaccgt cgccggtcat gtcgtgcggc
     3601 cgaccggtta aggaaacgtt cagccagagg gaaggagatc gccgtgagta ccaagcgaaa
     3661 ccgaagagag tttctcgcgg acgtgggacg agggactctg atcgcggcgc tcggttcgag
     3721 cgccgccgct gacctcggat tggccagcgc gggggtcgac agtccggagc cgcgtctgaa
     3781 cttccgggag ctggaaggct tggtcgcggt catgcaagag acgcccgcgc agcgtctcgt
     3841 ggccgtcgtg gtcgacgaac tgaagcgtgg ggtcgaattg cagcggctgg tcgcggcggc
     3901 tgcgcttgcc aacgcccgga cgttcggcgg tgaagattat gtcggctttc acacgatgat
     3961 ggcgatggca ccggcgtttc acatgagccg cgagttgccg gagaccacgc ggccgctgcc
     4021 cgtcttgaaa gtcctttatc gcaacaccag ccgcatccag gagagcggtg gacgaagcca
     4081 cgaggttctg cgtgcggtcg caccggtgtc tctctcccca gggtgtgtgg ggggcgaggc
     4141 cttgcgcgag cgggtgcgag ccaaggacat ggacgccgcc gagcacacgt tcgccgcgct
     4201 ggcggagcgc gggcccgagg aagcgttcaa cgaccttctg gtcgcagttc aagacaacgc
     4261 cgaagtccac cgcacggtgc tcccctaccg cgcctgggac ctgctcacct tgatcggtct
     4321 ggagcatgcc cacaccctgc tgcggcagtc agtccactat tgcgtcaaga gcgaacgcga
     4381 ctggaagcac acgcccgaga cggacaagcc ccgcgtcgtc ttgcccaaac tcctggacca
     4441 gtatcacctg gccggaaagg cgctcgggac tcggcagccc gacgccgcgt gggtcgagca
     4501 cctgagttac gccattttcg agggaaccgc cgatcaagcc gccgacgcgg cagcggccgc
     4561 cctggccgag ggcatggccc cggacgcggt cggcgaagcc gtctcgctcg cggccaacca
     4621 gttgatcctg cgcgacgccg gccgccgcgc ttccgaagct cagtccaaca agccagccgg
     4681 cagcgtccac ggtgattcga tcggagtcca cgcgtgcgac tcggccaatg cgtggcgaaa
     4741 catggccaag gtgagcaacc ctcgaaatac cgtcgcctgc ctgatactcg gcgcctacca
     4801 ggttgctcgt gaccgcgtgg agcgaggggg cgactttctc cactggtcgt ctcgaccgtt
     4861 ggcagccgat cttgatccca tcaaggaaag acagccagag gcgctcctca agcaactcga
     4921 cgcggcgatt cgtgccaacg atcagtcatc ggcctgcgcg gtcgttcatc aaatcggcgc
     4981 gctcgagcaa tcgccgcgag ccgtcttcga cctgcttttg cgctatgcgg tcagtgaaga
     5041 cggggcgctc cacgccgaga agtattaccg caccgtcacc gaagagtttg ccgccacccg
     5101 gccggcgttc cgatggcgac atctggtggc gctggcacgg gtaaccgcca gcgagtacgg
     5161 tcggccggcg gctggcctcg cggaggctcg agaacggcta ggaatcggtt gtgttccgtt
     5221 tgaacccaaa cgacccgttg gccaggtcca acgggtcgat ttcgaaccag attcgtccgc
     5281 tggttcacgc ttacacgttc acaacccggt gctgtatcga gaaccgccgc cccttaggcg
     5341 atttgtaatc gaaatatccc agtcccagcg cgccggccaa ggcgatgtgc tggggctggc
     5401 gtacatcaaa gccttcggtt ttgaggggat cgatggcctt accccccgcc gcggcgaccg
     5461 gaggaaggtt ttgcttgagc cgctgggcat cgacgatgtc ccactcgacg tgatcgaggg
     5521 ccacggggtc ggtcgcaaag aagatggagt tgttctccca cgtccactta ccttcccggc
     5581 gtgcgaaagg cccgccttgg tagacgccgc ggataccatc catgatctgc aggacgaact
     5641 tctgacgaat cataggatga gaaaccatct ggggaatgaa ctggttacag acgttggtaa
     5701 acggagtgct gtgtgatcga ttcacgttgt tgacagtccc gtggctcata ttcttcaagg
     5761 ccccggtcac tccggccgat ccgtggtctt tcacgaccgg aatggctacg atcttgttga
     5821 gagtcttggt gaccagtttg ccgagatgag agcggaattt gcgatcgtcc ttgggatctg
     5881 ccccataatc gatcaaatcc atttttacaa acgtatcatg atcgaaaccc gcgatcggat
     5941 cgctcctgaa tgacgggaaa tccagggtga actgattgcc ttcggggtcc agtccgcccc
     6001 a
//
LOCUS       DATAQL010001384         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4568376, whole genome shotgun sequence.
ACCESSION   DATAQL010001384 DATAQL010000000
VERSION     DATAQL010001384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4568376"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VHS97_23795"
     CDS             <1..964
                     /locus_tag="VHS97_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX3451298.1"
                     /translation="GTGGPEVLHIREIPTPEPRGEQVRVRVRACGLNRADLMQCRGYY
                     PAPPGAPADIPGLEYAGEVDALGPDVIGPLKLGDRVFGIVAGGGQAEYVLTHERLAVS
                     IPLNLDFVEAAAVPEAFITAHDALLTQGRLAPGERVLVHAAGSGVGTAAVQIAHAVGS
                     LVWGTVRTADKLERSKSLGLDHGVDNSNGGFAEVVASQTMGKGVNVVIDLLGGSALAG
                     NLAALAFRGRLVLVGLLAGTSGPLDLSLMLRKRITIVGTTLRARPLEEKIAATVRFAN
                     EVVPWLERGLVCPVVDSVFAFEDFANAQARVESNLVFGKVVLRL"
     gene            complement(1125..1835)
                     /locus_tag="VHS97_23800"
     CDS             complement(1125..1835)
                     /locus_tag="VHS97_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3451299.1"
                     /translation="MIEVVHVEKSYRSGSRLVPALRGISCSIDRGRFAFIVGPSGSGK
                     STLLYLLGAIDRPSAGKIIVNGQDLGRMSEAAQNVYRRDQIGFIFQSFNLISNLTALE
                     NVLVPFLPRGVTPELRQRGVDLLTEVGLGERLDHRPYQLSGGEQQRVAIARALVKQPI
                     LVLADEPTGELDSKSGDEIYTILRRLQESSRTTLVVVTHDRRFITPDDLVLEIQDGQL
                     AGRNGSASPALHQALITR"
     gene            complement(1853..3067)
                     /locus_tag="VHS97_23805"
     CDS             complement(1853..3067)
                     /locus_tag="VHS97_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3451300.1"
                     /translation="MGNFAWRNLLTRPMRTMLALVGLSIPILGVLGLFCLSNGLRDLV
                     GDTLSRIEGLIILRENVPSPVFSHLPASLVDDLKKIPGMRAVAPEVWGIAPTIEGRGM
                     LVSSLVSGKSMMNSLLDQPVVVGQDIVSHQNLHSAIFPRALKEKGTGRYLEPSDRGQA
                     NIVISRKIASQHPDAERNPKKVGDTLRIGGKPFQIIGIYETGSMILDVVIIMDIETAR
                     VVLNEPKDSISCIYVEGDNPAHNGDLAAAIEKAHPGFDARSMNEAQDNFSSMMGQIDM
                     FLLLTVSLALTVGIVGIINTMLMSTTERFVEFGVLRTNGWSQGNILALVTLESAYLGL
                     LAGLVGCLLAWVGTIVANRFITGGIHLGMTPLLAALGIGLSVMMGTLGGLYPAWRAAR
                     LMPMDAIRLGSH"
BASE COUNT          655 a         1037 c         1082 g          665 t
ORIGIN      
        1 cgggaccggc ggcccggaag ttctgcacat ccgcgagata cccacacccg agccaagggg
       61 cgagcaggtg cgcgtgcggg tgcgagcctg cggcttgaac cgggccgacc ttatgcaatg
      121 ccggggctat tatcccgctc cgcccggagc accggccgat attccgggac tcgaatatgc
      181 cggcgaagtc gatgcgctgg ggccagacgt gatcggcccg ctcaaactgg gagaccgcgt
      241 cttcgggatc gtggccggcg ggggtcaggc cgagtacgtc ttgactcacg agcgcctggc
      301 ggtatcgatc ccgcttaacc tcgactttgt tgaggctgcg gcggtccccg aggcgtttat
      361 caccgcgcat gacgcattgc tgacccaagg ccgtctggcg cccggcgagc gcgtgctcgt
      421 tcacgccgcc ggcagcggcg ttggcacggc ggccgttcag atcgcccatg ccgtggggtc
      481 gttggtctgg ggcacggtgc gtacagcaga caagctcgag cgatccaagt cgctcggatt
      541 ggatcatggc gtcgacaatt cgaacggcgg ctttgccgaa gtcgtcgcga gccagactat
      601 gggcaaggga gtcaacgttg tgatcgacct cttgggcggg tcggcgctcg cgggtaatct
      661 ggctgccctt gctttcaggg gccggctggt cttggtcgga ctgctggccg gaacgagtgg
      721 gcctttggac ctgagtctca tgctgcgtaa acggatcacg atcgtcggca cgaccctgcg
      781 cgcgcggccg ctagaagaga aaatcgcggc gactgtgcgg ttcgccaacg aggttgttcc
      841 ctggctcgag cgcggcctcg tctgtcctgt cgtcgattcc gtcttcgcgt tcgaagattt
      901 cgctaacgcc caggcgcggg tggagtcgaa cctggtcttc ggcaaggtcg tcctgcggct
      961 gtgaacggac gtgagggaac cacgaaagga cgagtgtgta gggtgggcac ctcccaccga
     1021 caggcgtctg gtgggcggtg cccaccctac gaaatccgac gaacgcaacg gttaattggt
     1081 cgtgatattt ctgcctggct cgtgagtgtc gctgccagcg gggttcagcg agttatcaag
     1141 gcctggtgaa gggccggcga tgcgctgcca ttgcggccgg cgagttgccc gtcctgaatc
     1201 tcgaggacaa ggtcgtcggg tgtgatgaag cggcggtcgt gggtcacgac cacgagagtc
     1261 gttcgggacg actcttgcag gcgcctgaga atcgtgtaaa tctcgtcgcc gcttttcgag
     1321 tcgagctctc cggtcggctc gtcggccagg accaggatgg gctgcttgac caatgcgcgg
     1381 gcgatcgcca cccgttgctg ttctccgccg gaaagctggt aggggcggtg gtcgagccgc
     1441 tcgccgagac cgacctccgt cagcaaatcg acgccgcgct gacgcagctc gggcgtcacc
     1501 cctcgcggta agaacggtac cagtacgttc tcgagagccg tcaggttgct gatcaggttg
     1561 aatgattgga agatgaaccc aatctggtcg cggcggtaga cgttctgagc cgcctcggac
     1621 atcctgccca agtcctggcc gttgacgatg atcttgcccg cactcgggcg atctatcgcc
     1681 cccagcaggt agagaagcgt gctcttaccc gagccgctcg ggccgacgat gaatgcaaac
     1741 cgaccccgat cgatcgagca actgattccg cgcaacgccg ggaccaaccg gctgccgctc
     1801 cggtaggatt tttcaacgtg taccacctcg atcattacga gacctctgtt cgttaatgcg
     1861 agcccaatcg gatggcgtcc attggcatga gccgggctgc gcgccaggca gggtagagcc
     1921 ctcccagggt acccatcatc accgacaaac caattcccaa tgcagcgagc aacggggtca
     1981 taccgaggtg aattcccccg gtaatgaacc gattggcgac gatcgtgccg acccaggcca
     2041 agagacagcc gaccaggccc gcaagcaaac ccagatacgc gctctcgagc gtgaccaagg
     2101 ccaggatgtt gccctgcgac cagccgttgg ttcggagaac gccaaactcg acgaaccgct
     2161 cggtcgtgct cataagcatc gtgttgatga tcccgacgat tccgacagtg agtgccagac
     2221 tgaccgtcaa caacagaaac atgtcgatct ggcccatcat tgagctgaag ttgtcctggg
     2281 cctcgttcat gctccgcgcg tcgaatccgg gatgcgcttt ctcgatcgcg gccgccaggt
     2341 cgccgttgtg ggcggggttg tctccttcga cgtagatgca tgagatcgag tctttgggct
     2401 cgttaagcac gacgcgcgca gtctcgatgt ccatgatgat gaccacgtcg agaatcatcg
     2461 agccggtctc ataaatgccg ataatctgaa acggcttgcc gccaattcgc agcgtgtcgc
     2521 caaccttctt cgggttacgc tcggcgtcgg gatgttggct ggcaatctta cggctgatca
     2581 cgatgttggc ctgacctcgg tcgctcggct cgagataccg tccggttccc ttctccttta
     2641 aagcgcgggg aaagatcgca ctgtgcaggt tttgatgcga gacgatgtct tgacccacga
     2701 cgaccggctg gtctaacagc gaattcatca tgctcttacc ggaaaccagg ctgctcacga
     2761 gcatcccccg cccctcgatg gtcggtgcga ttccccatac ctcaggcgcg actgctcgca
     2821 ttcccggtat tttcttcaag tcgtcgacca gactggccgg caagtggctg aaaaccgggc
     2881 tggggacgtt ctcgcgaagg atgatcaacc cctcgatccg ggagagcgta tctccgacca
     2941 agtcgcgcag tccattggaa aggcagaaaa gcccgagcac ccccaggatg gggatcgaca
     3001 gccccacgag cgccagcatt gtccgcatcg ggcgcgtgag caggttgcgc caggcaaaat
     3061 tacccatgat tcatccattt cgaggcaggg cagggcctat cgacacctcc gagtgtcggg
     3121 cggctcgacg gcagcatgca gttaacagca ttatagtcaa tacgtcgagt cggcataaga
     3181 cgagcgacag tgctgcgaga tcaacgtccg atggtggact ttgcgaacgc cgaacccaaa
     3241 aggcctaaac tagcaaatcg cacgccatcg tgagcagggg acgggcccgt acggcacaca
     3301 cgtcatgcag ccgagccggt ccgaatcgat gtcacccatt taaatccgtt tagaccgagc
     3361 tggacgccgc ctggttgtcg ttttccgtcg gcggagtgga cggcgtttcc gtggagtcag
     3421 ccgaatgctc ggacgaact
//
LOCUS       DATAQL010001385         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4109862, whole genome shotgun sequence.
ACCESSION   DATAQL010001385 DATAQL010000000
VERSION     DATAQL010001385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4109862"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="VHS97_23810"
     CDS             complement(<1..1857)
                     /locus_tag="VHS97_23810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3451301.1"
                     /translation="MIATISATRNIRRARSAPGMVGSRPLGPVDVLVLSAWCGLGGGL
                     LEVATTVCVRNLIATTRGYLMTRHFVWLAPLSNLMFFSAMGLLLAAIVWMWPRRGGWL
                     VPRFITFLAVLPVLMVTSTKLYPIAWTILAAGIASCIVPILEWNAPRLRRQLLLSFPG
                     LLVLVLIPAGWVVGGEWLMERHESGRPSPPGDPPNVLLITLDTVRADHLSVYGYERPT
                     TPTLERLAKKGIRFGGARATAPWTVPSHASIFTAHWPHELGVNWDTPLDEKYPTLAES
                     LGSLGYATAGFVANTMECSYDRGLSRGFTHYDDYSLEHLLPFRTAWLVDHFMQAVSDA
                     GVFVGRAFDIGPFRPMQDSWVARLFIRWPRKDAGSINHAFFDWLSQRREPGRPFFAFL
                     NYFDAHAPYVLPVGAEYRFGLVPRRPADFMFLMEYWETIDKLTLRPVYRDLARDSYDN
                     CLAYLDQRLGELFNELERQGVLDRTLLIVTADHGEGLGEHGLFDHGGSLYRSEIQVPL
                     LIVLPAGRRSNGIVHETVSLRDLPATILDLVGLEKGSPFPGRSLVHLWRDPSRGAGSA
                     DVGGAISELPKPNPYDPNHGRSPAEHGPLTSLAEGDFVYIRNDGDGTEELFNE"
     gene            2323..>3015
                     /locus_tag="VHS97_23815"
     CDS             2323..>3015
                     /locus_tag="VHS97_23815"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3451302.1"
                     /translation="MERSIHLSVDRVPLLGTTPVTIVPGLHLLGGLEPAAAYVVETSL
                     GLVLVDSGLRGDAGVLKSQLASLGLDWRQVRAVLLTHVHGDHCGGAEYLRAVLGAKVY
                     AGQGDAAVLRAGGPHEAFFSAFYMPDDNPHPTTVDVELKGGESLVFGDVRFLALATPG
                     HTSGSTCYLMERAGYRALFAGDVIAMLRGDPQSHSQNRRPLGTYSVYLPPRYRGDARA
                     YLTSLRALRALPV"
BASE COUNT          611 a          891 c          947 g          566 t
ORIGIN      
        1 ctcgttaaac aattcctcgg tgccatcgcc atcattgcgg atatagacga aatcgccctc
       61 ggccagggag gttagagggc cgtgctcagc cggtgatctg ccgtgattcg gatcatacgg
      121 gttgggcttc ggcagttcgg agatcgcccc gccgacgtcg gccgaacccg ccccgcgcga
      181 agggtcacgc cacagatgca ccagcgaccg gcccgggaag ggcgatccct tctccaagcc
      241 aacgaggtcg aggatcgtcg ctggcagatc gcggaggctt acggtttcgt gaacgatccc
      301 attggacctg cgaccggcgg gcaagacgat aaggagcggc acctggatct ccgagcggta
      361 caggctccca ccatggtcga agaggccatg ctcccccagc ccttccccgt ggtcggcggt
      421 tacgatcaat agcgtccgat cgagtacgcc ctgacgttcc aattcattga acagttcgcc
      481 caatcgctgg tcgaggtagg ccaagcagtt gtcgtatgag tctcgggcaa ggtcccgata
      541 caccgggcgc aacgtcagct tgtcgatcgt ctcccagtac tccattagga acataaaatc
      601 ggctggcctc cggggtacca gcccgaagcg atattcggct cccacgggca ggacgtaggg
      661 ggcgtgagca tcgaaataat tgaggaaggc aaaaaacgga cgtccgggct cacgcctctg
      721 cgacagccag tcaaaaaatg cgtgattgat cgatcctgcg tcctttcttg gccaccgaat
      781 gaacagtctc gccacccagg agtcctgcat ggggcggaat gggccgatgt caaaggcgcg
      841 ccccacaaat actcctgcgt cggagacggc ctgcatgaag tggtcgacta gccaggccgt
      901 tcggaacggc agcaggtgtt cgaggctata atcgtcgtag tgggtgaatc ctcggctcaa
      961 cccccggtcg tatgagcatt ccatggtatt ggcgacgaag cccgctgtgg catagccgag
     1021 agatcccaat gactcggcca aggtcgggta tttctcatcc agcggagtat cccagttcac
     1081 gcccaactca tggggccagt gggcagtgaa gatgctcgca tgcgaaggga ccgtccaggg
     1141 agctgttgcg cgcgccccac caaagcgaat gccctttttg gccagccgct cgagcgtcgg
     1201 ggtcgttggt cgttcatagc cgtacacgct tagatggtcg gcacggacag tgtccagcgt
     1261 gatcaagagc acgttcgggg ggtcgcccgg cggggagggg cggccggatt catgtcgctc
     1321 catcagccac tctcccccga cgacccaacc tgccgggatc aggaccagga cgagcaagcc
     1381 ggggaagcta aggagcaact gcctgcgtaa gcgcggcgca ttccactcga ggattggaac
     1441 gatgcacgaa gcgatccctg ccgccaggat cgtccaggcg atcggataaa gtttcgtgct
     1501 ggtcaccatg agcacgggca agacggcgag gaaggtaatg aaccgcggga cgagccaccc
     1561 cccgcgcctg ggccacatcc acacgatagc cgcaagcagt aatcccattg ccgagaagaa
     1621 catcaagttc gatagcggtg cgagccagac gaagtgccgg gtcatcaagt atccccgggt
     1681 ggtcgcgatc aagttcctga cacagaccgt cgtcgccacc tcgagcaacc cgccgcccaa
     1741 accgcaccaa gcggacagga cgagcacatc cactgggccc agcgggcggc tgcccaccat
     1801 gcctggggcg gatcgggcac ggcgaatgtt ccgagtggcc gaaatggtcg cgatcacgag
     1861 gcgtgttctc cgcggattaa cctaaccttc tcaggaagga attaggatat acgtgcacta
     1921 aggaattgag atatacgacc aagaaggaat taggatatag gcgttttccg ccccgtttcg
     1981 gccgggcatc tactaattat ttcagtaatt tcttcgcttg aatcgtcgct gtagcgagta
     2041 gaagtggcgg aagtgcccgt gataccttga gaggggtcgc gccaaaatcg gagtgcgggc
     2101 atgagccgcc ggtgagctgg aaatccgcgg cggaatcgga ccggccatcc ccgaaccgct
     2161 cggctcggcc ataatcgcgt gcgtcgcggc gttggtttga cgatgaggaa catcgtgcgg
     2221 gcatccgtgg ttcgattgaa gagttcacgc gggcgatggg tggcgagtct gatcgtatta
     2281 atcggggtct cgctccttgc tttcgcgtac cttcgacatc gaatggaaag gagcattcat
     2341 ctatccgtag accgggtgcc gctcttgggc acaacgccgg tgacgatcgt cccaggactt
     2401 caccttctcg gagggctgga gccagcggcg gcttatgtgg ttgagacttc ccttggactg
     2461 gtcctggtcg attcaggact ccgaggggac gcaggcgtcc tcaagtcgca gcttgccagc
     2521 ctggggcttg attggaggca agtgcgtgcc gtcttgttga cccatgtcca cggcgatcat
     2581 tgcgggggag ccgagtacct gcgggcggtg ttgggggcaa aggtctacgc gggccagggt
     2641 gacgccgcgg tcttgagggc gggcggacct cacgaggcgt tcttcagcgc cttttacatg
     2701 cccgatgaca atccccatcc gaccaccgtc gacgtcgagc tgaaaggggg tgagtcactc
     2761 gtttttggcg acgtccgatt tcttgcactg gccacgcccg gccacacgtc gggaagcacc
     2821 tgctacttga tggagcgagc agggtaccgc gcgcttttcg cgggcgatgt gatcgcaatg
     2881 ttacgtggcg atccgcaatc ccactcccaa aatcgacgac cgctcgggac gtattcggtc
     2941 tacctgccac ctcgctatcg gggcgatgcc cgagcctacc tgacctcgct gcgagcgctg
     3001 cgagcgctgc ctgtc
//
LOCUS       DATAQL010001386         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3349091, whole genome shotgun sequence.
ACCESSION   DATAQL010001386 DATAQL010000000
VERSION     DATAQL010001386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3349091"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VHS97_23820"
     CDS             <1..811
                     /locus_tag="VHS97_23820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3451303.1"
                     /translation="FGPLLGGLLLCLPLADTWRLRVPFLVAAAFSTMAWVLVLTRLPE
                     SRHPGQPAREIARVASWKGIVATISIPGIGRLILIGFLAVFAFAAFEGTFALFLQRRM
                     HWDERNAAFAFAGIGFLSAVVQGGLIRRLVPRFGEARLIIAGLVLAACGFVGMSLVAN
                     ALELAGSMVLLGVGQGLLSPSVSGLLSRYTPMSAQGTVFGTLTSAQTLARMISYSASN
                     ILLGKVSTSAPYWGAFGVELLALAAATRGGTNVHEQSRPVLFSGLERREGR"
     gene            complement(776..3256)
                     /locus_tag="VHS97_23825"
     CDS             complement(776..3256)
                     /locus_tag="VHS97_23825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3451304.1"
                     /translation="MREAETIPIDKSTARDAQRLRPALADATQLSESVGEISRRPLGG
                     LGLKTWIVLVISLAVLVPTAGDFGVTWDEPQYRHCQLLAVQWCEQLAKVRSWHDVTEV
                     LDPITLLYYWDYGHHGISFHPPLAGQLNLAAHALFGHWMKDIPARRMASIIEFALTIT
                     IGFHFLSRRYGEWVGLVMAGSLLFMPRLYGQAHLMDTDIPGLLLWAAAALAFWKGLYE
                     PGARRWRVAVGILLGLAFVEKMGAVVVLLPLLLWLVVGHLPRSFARPGARANWIDGTL
                     TMGAMLVPLGLAFQQIQILQQQLPPPAQTNLFVHRPVSDWPGAILAIPLAVWIVRRLL
                     GGFFPNNKVWGVERPALETWTAILAFAPVVGWLGNPSWWREALPRLAHYYTLSLDREL
                     SLPRIQIMYFGQIYEYSLPWHNAWTLIAITVPVAILGASVIGLLWAIGQIRRDRLPLY
                     FVVHFLTLPVIRMLPTPAHDGVRLFLPTFFFLAGFAGWGTVWVADILARGVRIPTWIS
                     RLALAGAVLGSSAVSLYRIHPYELSYYNELIGGPRGAWERGFELSYWYDAFNDQVIDD
                     LNRRLPPGAKIDFLNEMTKTAVGTFYLRKDLGSLRRDIILGRPDLPFPFVWLLAQDSK
                     TTAFTRLLFAMQPWYASEPRQLDGARIVSVDDPVAVSRAWALFVLLDAADRSPKDPDA
                     APEWIRDHTPWLARLWGDKLIKAHRLAMNQAVLAWSRSDPDGLLAAARVIASRQPIEN
                     GTPAEQLMRLMTTESTPRPSEQVRHILMAQLLDARPEALVEAVQILNAHRDEIVKVMT
                     RYGYTDPKAIGGYLDRDLPTFAALETGK"
     gene            3643..3957
                     /locus_tag="VHS97_23830"
     CDS             3643..3957
                     /locus_tag="VHS97_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451305.1"
                     /translation="MKRHLTTLVLGGVLGSMVLVGNAEACHKKKCGHAPVCAAPAPVA
                     YTCAPKIKHCGFNFKLPTLCHKRAVTTVACATPVYRAPAPVCYSAPVGYPIASPQTSA
                     QH"
BASE COUNT          841 a         1277 c         1286 g          801 t
ORIGIN      
        1 attcgggccg ctcttgggcg gactgctctt gtgcctgcct ctagccgata cctggcggct
       61 tcgagtgccg ttcctggtgg ccgcggcatt ctcgacaatg gcgtgggtgc tcgttctgac
      121 gcgcctcccc gagtcgcggc acccgggcca gcccgcgcgt gagatcgcgc gggtggcgag
      181 ctggaaggga atcgtcgcta ccatatcgat tcctgggatt ggccggctca ttctgatcgg
      241 ctttctcgcc gtctttgcgt tcgcggcgtt tgagggaacc tttgcactct tcttgcagcg
      301 ccgcatgcat tgggacgagc gcaatgccgc atttgcgttt gccggcattg ggtttttgag
      361 cgcggtcgtg caggggggcc tgatccgccg cctcgtcccg cgctttggcg aagcgcggct
      421 cattatcgcc ggcctggtgc tggcggcgtg cggattcgtc ggcatgtcgt tggtggcgaa
      481 cgctctggag ttggctggct cgatggtgct cctgggcgtc ggccagggac ttctcagtcc
      541 gtccgtctcg ggcttgctct cgcggtatac accgatgagc gcccagggaa ccgttttcgg
      601 gacacttaca tcggcgcaaa cgctcgctcg catgatcagt tattcggctt ccaatatctt
      661 gcttggaaag gtctcgacca gtgcacctta ctggggcgcg ttcggagtcg aactgctcgc
      721 cctggccgcg gccactcgtg ggggcacaaa cgtccacgag caatctcgtc cagtgctatt
      781 ttccggtctc gagcgccgcg aaggtcggta gatctcgatc aagatatccg ccgatcgcct
      841 tgggatcggt gtacccgtat cgggtcatga ctttgacgat ttcgtcacga tgggcattca
      901 gaatctgcac cgcctcgacg agggcctcgg gacgggcgtc gagtaattgc gccatgagaa
      961 tatgtcgtac ctgttcactg gggcggggtg tggactccgt agtcattagt cgcatgagtt
     1021 gttccgccgg agttccgttt tcgatcggct gacgtgacgc gatcacccgc gcggctgcga
     1081 gcagcccgtc ggggtcggat cgcgaccagg caaggacggc ctgattcata gccaggcggt
     1141 gggccttaat taatttatcg ccccagagcc gcgcgagcca aggcgtatgg tcgcggatcc
     1201 attcaggagc cgcgtccggg tctttcgggc tgcggtcggc cgcgtcaagc agtacaaaga
     1261 gcgcccacgc gcgcgatacg gctacaggat cgtccacgga aacgatccgc gcgccatcga
     1321 gctggcgtgg ctcgctcgca taccagggtt gcattgcgaa caagagccgc gtgaaggctg
     1381 tcgtcttcga gtcctgggcc aagagccaga cgaaaggaaa cgggagatcg ggcctgccga
     1441 ggatgatgtc gcgtctcaac gatccaaggt ctttacggag gtaaaacgta ccgaccgccg
     1501 tcttggtcat ctcgttcaga aagtcgatct tggccccggg tgggagtcgg cgattgagat
     1561 cgtcgatgac ctggtcatta aacgcatcgt accagtaact cagctcgaag ccccgttccc
     1621 acgcaccgcg tgggccgccg atcagctcgt tgtagtaaga cagctcataa ggatgaattc
     1681 gatagagcga gaccgctgat gagccgagca ctgcccctgc taacgccaac cgggaaatcc
     1741 acgttgggat gcgaacccct cgcgcgagaa tatcggccac ccagaccgtt ccccagccgg
     1801 cgaaccccgc cagaaagaaa aaggtgggca gaaacagtcg cactccatcg tgcgcgggcg
     1861 tcggcaacat gcggatcacg ggcaaggtca ggaagtggac gacaaagtac agtggcagcc
     1921 ggtcgcggcg tatctgccca atcgcccaca gaagcccgat cacgctggcg cccaggatcg
     1981 caacgggcac ggtgatcgca atcaaggtcc atgcattgtg ccaggggaga ctgtattcgt
     2041 agatttgacc gaaatacata atctggatgc gagggagcga gagttcgcga tcgaggctca
     2101 gcgtgtaata atgggcgagc cgcgggagcg cctcacgcca ccagctcgga ttgcccagcc
     2161 agccgaccac gggagcaaag gccaggatgg ccgtccatgt ctcgagtgcc ggccgctcga
     2221 ccccccaaac tttgttattg ggaaagaacc cgcccagaag gcggcggacg atccagacag
     2281 ccagcggaat tgcgagaatc gctccgggcc agtcactaac gggtcgatgc acaaaaaggt
     2341 ttgtttgggc gggagggggc agttgctgct gcaggatctg gatttgctgg aaggccagcc
     2401 ccagcgggac caacatcgcg cccatggtca gcgtaccatc gatccagttc gctctcgcac
     2461 ccggccgcgc gaaggatctc ggtaaatgcc cgacgaccaa ccagagcaag agcggtaaga
     2521 gcaccacgac cgcgcccatc ttctcgacga acgcgagacc gagcagtatt ccgaccgcga
     2581 cccgccatcg ccgcgcgccc ggctcgtaca atcccttcca gaatgccaac gccgccgccg
     2641 cccacagcaa gagccccggt atatcggtat ccatcaggtg cgcctggccg taaagacgcg
     2701 gcataaacaa gagcgaaccg gccatgacca gcccgaccca ttcgccatag cgccgtgaca
     2761 ggaaatgaaa gcctatcgtg atcgtcagcg caaattcgat gatcgatgcc atccgccgcg
     2821 ccgggatgtc cttcatccaa tgaccgaaca gcgcgtgcgc ggccagattc aactgaccgg
     2881 ccagtggggg atgaaagctg atgccatggt ggccgtaatc ccagtaatac aataacgtga
     2941 ttggatcgag aacttccgtc acgtcgtgcc aggaccggac ttttgccagt tgctcgcacc
     3001 actggaccgc caacaactgg caatggcggt attgaggctc atcccaggtc acgccgaagt
     3061 cgcctgctgt cggcaccagc acggcgagag aaattaccag aacgatccag gtcttcaagc
     3121 caagaccgcc gagcgggcgt cggctgatct ccccgacact ttccgatagc tgggttgcgt
     3181 ccgcgagggc cggacgcagc ctctgggcgt cgcgggcggt ggatttatcg atcgggatcg
     3241 tctcggcttc gcgcatgttt gggatccgta ccgctcgctg gggtcaatct cgtcggcgac
     3301 gactgctcag gtcggcagcc gcgaacgcca caggtgagcc cgccccgcca ttcccactct
     3361 gcctcgctct acctttggat tatcggagcc cttggtttga cacggcaagc ggatacttcg
     3421 attcccggct cacattgggc gactgcctag gaggtcgaca aaccggtaaa agccgtgaac
     3481 tcggcaagat ccgtgttcta ggatcaataa ggttgaaagt tacaggccga ttatttaccc
     3541 tgctttgcgg gaaactcctg agtttcccag ttatgacacc tgtcgatcgg atgcttggat
     3601 gttcgagcac cgtcggcaca accgaaataa ggagatgtat ccatgaaacg acacctgacg
     3661 actttggtcc tgggcggagt cttgggatca atggtcctcg tcggcaacgc tgaagcgtgc
     3721 cacaaaaaga aatgcgggca tgcacctgtc tgcgcagcac cggcgcccgt cgcctacaca
     3781 tgcgcgccca agatcaaaca ctgcggcttc aacttcaagc tgccgacatt gtgccacaag
     3841 agggccgtaa caaccgtagc ttgcgcaacg cctgtgtacc gcgcacctgc accagtttgt
     3901 tattccgcgc cagtgggcta ccccatcgct tccccccaga cttcggcgca gcactgacac
     3961 ttggcggcgc cggttcaaac acgcacggca acgattcact ccgaacaggc cggtcgggcg
     4021 gggagcgcat cgcttcaacg tccaatcgga atggtctgag cctgaattcg ggctcgcgcg
     4081 ttcagtcgta agaactcgct gtaggggaat atccatacca agggaacgcc cagcttggtg
     4141 actgtgtagg cggggcgacc ctgggcagcg agggcccggt cggcgtctaa aaaggcgccc
     4201 ggccg
//
LOCUS       DATAQL010001387         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4720600, whole genome shotgun sequence.
ACCESSION   DATAQL010001387 DATAQL010000000
VERSION     DATAQL010001387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4720600"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..528)
                     /locus_tag="VHS97_23835"
     CDS             complement(331..528)
                     /locus_tag="VHS97_23835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451306.1"
                     /translation="MKDIGRRVAVGIDTKPIVVWGTGFVGRIVIAEIVEHPLFELVGV
                     GGEQPREGGPRRRRDLPGLRS"
     gene            complement(548..1780)
                     /locus_tag="VHS97_23840"
     CDS             complement(548..1780)
                     /locus_tag="VHS97_23840"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX3451307.1"
                     /translation="MDIADMITRFDHHDPVIMADPAAAYRRFRNECPVGWSNVHQGFW
                     VVSRYDDVLHAAGDAEQYCSGQGIAIPGTSTGARIEPQEADGPRHAQIRKQMNAWFAR
                     GPVLAMRPAIEDLSASLLEQCRTRITFDLAIDYTVPLASLTMLRVLGIPIEYEGRLRK
                     NLDVLLHARTAEESVVFGSYIELYECISEVLVGKQKGAEGNPGAEDDLINKIMAVEID
                     GTPTTVAEQVSMILSLVLAGLETSALAIASTAACLIDQPELRDRLIADPRLIPKAIDE
                     FLRFISPVQAIGRRVTSQAQLGERTLNGGDQVLLLYGSANRDPAIFNEPDEIVLDRHP
                     NRHVTFGYGVHRCLGHHVARLELDVALRDMLGLLPALAPQGRVEWSFGENRGIRSLPV
                     TLVHEASDAVRETPRVPA"
     gene            complement(1854..2438)
                     /locus_tag="VHS97_23845"
     CDS             complement(1854..2438)
                     /locus_tag="VHS97_23845"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3451308.1"
                     /translation="MCSCDERAIERILFRYARCVDSADWDCLGELFRYGQVVTEGVDG
                     VAVGPDAVRDVWRTVNRVHPDGTLRTRHLLTNVIVDVSPDDDIANADAYFMVFQATPE
                     LPLQPIAGGWYEDTFHKIDGVWWFKQKKIHVSLVGDVSDHLSLAFDSAGQLSDPPPAD
                     LLAAASGRSPADPANSPDSEPGKGCLPLWEDRLR"
     gene            2660..3556
                     /locus_tag="VHS97_23850"
     CDS             2660..3556
                     /locus_tag="VHS97_23850"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3451309.1"
                     /translation="MTRPALSAARSVDVLNFMASRPRESFTLSELSRQLGISTASGHA
                     VLQVLTAANYLVRDPVQKRYLLGPTLIALGHSALESHPAIDRARDEVRHLADSLQLEC
                     LAEIVCDDEIMILARAGRPRKFEFLPRVAQRLPLIPPVGTALCAWADEPTTKKWLDRL
                     GPIPTQEMAHITQAIEGIRARGYDVGLETPTRKQIADLLARRAHEADPKEWEPMLADL
                     FKTLAREENQIVGPIDPRREYLVNYIMAPVVNNRGEALMCLTVLGFEDPLSGEEIQKI
                     GQVVVQAGALAGIAGREASTYP"
     gene            complement(3721..>4315)
                     /locus_tag="VHS97_23855"
     CDS             complement(3721..>4315)
                     /locus_tag="VHS97_23855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3451310.1"
                     /translation="GIMPAGRFVEEDDAVTDAIIDINVKGLLHGTKLALPGMLERGSG
                     HIVNVASYAGKFPAAGLATYCASKHAVVGFSESLRDELAATGVTVTAVLPSAVRTDLV
                     AGVQLGGLLPTVDPERIADAVVASCRHRSAIVAVPAWMRSYEAAAALVPDRLLGAIRG
                     RLTRDRALRTLDTGARADYDARVRRDASAPANTQPTR"
BASE COUNT          804 a         1360 c         1357 g          794 t
ORIGIN      
        1 gcgagatcgc cggccagggc acgaaagtgg gcgcagacct gcggcgcctg gccgaccacg
       61 acgtcgatga ggtgttcttc gaaaccggcc gcggcccgat gcccatcgcc gagcaactcg
      121 atctggttga tgactggcgg agcgagttga gtgcctgacc tcagcgaagt tgcaggcggc
      181 cccgacatgc cgctaccgga cgcgatgacg acgcagcgtt ccatccggca ccgtgatcgg
      241 caagtcacga tgacctgacc ggtgcggccg gtgcgagcgg gaactcagcc gggccgaacg
      301 gcacgtcatc tacaacgacg gcgccaacgt ttatgagcgc agacccggca gatctcgccg
      361 acgtcgcggc ccaccttctc ggggttgctc acccccgacg ccgaccaact cgaacaacgg
      421 atgctcgacg atttcggcga tcacgatcct gccgacaaaa cccgtgcccc agaccacgat
      481 tggcttggtg tcaatgccaa cggctacgcg tcgcccaatg tctttcactc aaccctcgtc
      541 aagactttca cgccggaacc ctcggtgttt ccctgactgc atccgatgct tcgtgaacaa
      601 gcgtcacggg tagggatcgg atcccgcggt tctcgccgaa cgaccactcg accctgcctt
      661 gcggtgcgag cgctggcagc agacccagca tgtcgcgcag cgcgacgtcg agttcaaggc
      721 gagccacgtg atgccccaga catcgatgaa cgccatagcc gaacgtaacg tgacgattcg
      781 gatgccgatc gaggacgatc tcatcgggtt cgttgaatat cgccggatca cggtttgccg
      841 agccgtagag aagcaggact tggtccccgc cattgagggt cctttcgccg agctgcgctt
      901 gggatgtgac ccttctgcct atcgcctgga ccggagaaat gaatcgaagg aattcgtcga
      961 tagccttcgg aattaggcgt gggtcagcga tcagccgatc tctgagctcg ggctgatcga
     1021 tcaaacatgc cgcggtcgac gcgatggcaa gggcactggt ctcgagcccg gcaagcacaa
     1081 gactcaagat catggaaact tgttcggcaa cggtggttgg cgtgccatcg atttccacgg
     1141 ccatgatctt gttgatgagg tcgtcttcgg ccccgggatt tccctcggcg cccttctgct
     1201 tcccaaccag aacctcgcta atgcattcat acagttcgat atacgaaccg aatacgacgc
     1261 tctcttccgc cgttctcgcg tgcaagagca cgtccaggtt ctttcggagc cggccttcgt
     1321 actcgattgg tattccaaga actcgcaaca tggttaacga ggccaggggc accgtgtagt
     1381 caattgcgag gtcgaatgtg atgcgcgttc tgcactgttc cagcagagag gccgataggt
     1441 cttcgatagc cggccgcatc gccaggactg gaccccgagc gaaccacgcg ttcatctgct
     1501 ttcgaatctg tgcatgccgg gggccgtctg cttcttgcgg ctctatccgc gctcccgtcg
     1561 aagtgccggg tatcgcgatt ccctgaccgg agcagtactg ctcggcatca ccggccgcgt
     1621 ggagcacgtc gtcgtagcgc gagaccaccc aaaaaccctg gtggacattc gaccacccga
     1681 cggggcattc gttcctaaag cggcgatagg ctgcagcggg atccgccatg attaccggat
     1741 cgtggtgatc gaaacgagtg atcatgtctg caatgtccat ggcgaccagc tcctcctggt
     1801 tgaagtttcg atagggaagg gcgaagaatg ctggaactga ccgttattcg tggctaacgc
     1861 agtcggtcct cccagagcgg gaggcacccc ttgcccggct ccgaatccgg cgagttcgcc
     1921 ggatcggcag gcgaccggcc ggaagcggcg gccagcaggt cggccggcgg gggatcgctg
     1981 agctggccgg cgctgtcgaa ggccaaactg agatggtcgc tcacgtcgcc gaccaggctc
     2041 acgtgaatct tcttctgctt gaaccaccac actccgtcga tcttgtggaa cgtgtcctcg
     2101 taccacccgc cggcgatcgg ttgcagcgga agctcagggg tggcttggaa aaccatgaag
     2161 tacgcatcgg cgttcgcgat gtcgtcgtcg ggggatacgt cgacgataac gttggtgaga
     2221 agatgtcgag tccgtaacgt gccgtcaggg tgcacccggt tcacggtgcg ccagacgtct
     2281 ctgacggcgt ccggacccac ggctaccccg tcgacacctt cggtgaccac ttgtccgtac
     2341 cggaagagct caccgaggca atcccaatcg gccgaatcga cacatcgcgc gtagcggaac
     2401 aagatccgct cgatcgctcg ctcatcgcag ctacacataa gtctccttcg gtaggtcttc
     2461 ttcagggctc agccgagaaa cgtgcatggc gtgcccggaa ctgagccgca cgactttcga
     2521 acgtctgcgc ttaacccgcc catcttatcg taatatggag aagtctctcg ctatttcgag
     2581 atgatcgtag gtgggatact tggtccgcgc aagtcaatgc gccggcccaa cccgtgacga
     2641 gaggaaccga ccaccaccca tgacccgacc tgctctctcc gctgcgcggt ccgtcgacgt
     2701 cttgaacttc atggcttcca ggccacgcga gtcattcacg ctctcggagc tgtcgcgaca
     2761 gctcggcatc agcacggcgt cgggacatgc cgtgttgcag gtactgacgg cggcgaacta
     2821 tctggtgcgc gatcccgtac agaagcgcta ccttctcggc cccacgttga tcgccctcgg
     2881 acactccgcc ttggaatcac atccggcgat tgaccgggcc cgtgacgagg tccgacacct
     2941 cgccgattcg ctccagcttg agtgcctggc cgagatcgtc tgcgacgacg agatcatgat
     3001 cttggcgcga gccgggcggc cacgaaagtt tgaattcctt ccccgggttg ctcagcgtct
     3061 gccgctgatt ccgccggttg gcaccgccct ttgcgcctgg gccgatgagc ccaccacgaa
     3121 gaagtggctc gatcgactcg gcccgatccc gacacaagag atggctcata tcacgcaggc
     3181 gatcgagggc atccgagccc gcggctacga cgtgggcctt gaaacgccca cacgaaagca
     3241 aatcgccgat ctgctagctc gacgtgcgca cgaggccgat ccaaaggagt gggagcccat
     3301 gctcgccgat ctcttcaaga ccctcgcgcg ggaagagaac cagatcgtgg gcccgatcga
     3361 tccacgtcgg gagtacttgg tcaactacat catggctccg gtggtcaaca accgcggcga
     3421 agccttgatg tgccttacgg ttctcgggtt cgaagatcca ctcagcggcg aggagatcca
     3481 gaagatcggc caggttgtgg tgcaagctgg cgctctcgcc ggaattgcgg gacgcgaggc
     3541 gtcgacctac ccgtagcacc atcattggtg acctcgctcg tcttggatgg aaaagtcgac
     3601 cgaggcggcg ggggctggcc gaaggttccg cgacgggtgg cactggccat tccgctgtcg
     3661 acacggccgg ccgggtctgg gcgctgagac gacgaacacg tcggcaggga gctggtcaga
     3721 ttagcgtgtc ggttgagtat tggcgggggc gctcgcgtcg cggcgcaccc gggcgtcgta
     3781 gtcggcgcgg gcgcctgtgt cgagggtgcg cagcgcgcgg tcgcgggtca ggcggccgcg
     3841 gattgcgccg agcagccggt ccggaacgag agcggcggca gcttcgtagc tgcgcatcca
     3901 ggctgggacg gcgacgatcg cggaccggtg cctacaggag gccacgaccg cgtcggcgat
     3961 ccgctcgggg tcgacggtgg gcagcagccc gccgagctgc actccggcga cgaggtcggt
     4021 ccgcaccgcg gatggcagca ccgcggtcac ggtgacgccg gtggcggcga gttcgtcacg
     4081 caacgattcg ctgaatccca ccaccgcgtg cttgctggcg cagtaggtcg ccagcccggc
     4141 ggcaggaaac ttgccggcgt aggacgcgac gttgacgatg tggccgctgc cgcgctccaa
     4201 catgccgggc agcgccagtt tcgttccgtg cagtaggccc ttgacgttga tgtcgatgat
     4261 cgcgtcggtg accgcgtcgt cctcttcgac gaaccgcccg gcgggcatga tgccg
//
LOCUS       DATAQL010001388         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4263980, whole genome shotgun sequence.
ACCESSION   DATAQL010001388 DATAQL010000000
VERSION     DATAQL010001388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4263980"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2040
                     /locus_tag="VHS97_23860"
     CDS             <1..2040
                     /locus_tag="VHS97_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3451311.1"
                     /translation="RFVIYADYFTRPFTGRILRSMKAIPISPSGGPKMILQAFREAGR
                     ALDAGELVCLFPEGQLTRNGVMAPFQRGLERIVKGRTTPIIPVHLDRLSNSVFAPASR
                     RRIPKQIPYPVTVSIGSPLPANVPLYQIRQTIRELDTKAWDYRKADCRPLHHGFIHRA
                     RRHPARLAFADLLTPKVSYFRALAGSIAIARAIKRRWEGQANVGVLLPASVGGALVNL
                     AASLAGKAVINLNFTTGRTGMESAAAQAGLKTIVTSRTFLEKAKLESPLGTELIYLED
                     VMKTITRKDRLVAMVLAILAPIGTIERLAGAAKKPTVDDTATIIFSSGSTGEPKGVVL
                     SHFNIDSNVEAIGQVYRVLKNDRLIGILPFFHSFGYTMFWFAANSGIGTACHPSPLDA
                     AVIGSLVERYRVTVLLATPTFLQLYMRRCTPAQFGSLRLVLAGAEKLPESMALAFEDT
                     FGIRPMEGYGLTECSPVVAVNTFDWREPGFFQPGSRRGYVGQPLPGVSVRIVSSETYE
                     PLGPDCPGLVLVKGPNVMQGYLGRDDLTKAAFHEGWYVTGDQGFVSEDGFLKITGRLS
                     RFSKIGGEMVPHGLIEELLQQGVAADEQVFAVTAVGDERKGEKLVVLHTLDDEKVDQA
                     LERLGSHGLPNLFIPRRDQFIKVDKLPMLGTGKLDLRGMRRVAEEAMKGREAVSV"
BASE COUNT          428 a          657 c          718 g          443 t
ORIGIN      
        1 cgattcgtga tctacgccga ctacttcact cgtcccttta ccggccggat cttgcggtcg
       61 atgaaggcga ttccgatctc ccccagcggc ggaccgaaga tgatcttgca ggcgtttcgg
      121 gaggcgggcc gagcgctcga cgcgggagag ctcgtctgcc tgtttcccga gggccagctc
      181 acgcggaacg gcgtaatggc tccgtttcag cgcgggctgg agcgaatcgt caaagggcgc
      241 accacgccga tcatcccggt gcatcttgat cgactcagca acagcgtctt cgcgccggcc
      301 agtcgtcggc ggatacccaa acagattcct tacccggtta cagtctcgat cggatcgccg
      361 ctgccggcaa acgttcctct gtatcagatc cggcagacga ttcgcgagct cgacaccaag
      421 gcgtgggact accgcaaggc ggattgccgc ccgctgcacc atgggtttat ccaccgggcg
      481 cggagacatc ccgcgcggct ggcgtttgcg gacctcttga cacccaaggt ctcgtacttc
      541 cgtgccctgg ccggctcgat cgccatcgca cgggcgatta agaggcggtg ggagggtcag
      601 gccaacgtcg gtgttttgct gcctgccagc gtgggcggag ctctggtcaa cctggcggcg
      661 tcgctggccg gcaaggccgt gatcaacctc aacttcacca ccggccgtac cggcatggaa
      721 tcggcggccg cgcaggcggg tctcaaaacg atcgtcacca gccgcacgtt cctcgaaaag
      781 gccaagctcg agtcgccgct agggactgag ctcatctatc tcgaagacgt gatgaagacg
      841 atcacgcgga aggaccggtt ggtcgccatg gtcctggcaa ttcttgctcc aattggcacg
      901 atcgagcgac tggcaggggc ggcgaagaaa ccgaccgtcg acgacacggc gaccatcatt
      961 ttcagcagcg gcagcacggg tgaacccaag ggggtcgttc tatcgcactt caacatcgat
     1021 tcgaacgtcg aggccatcgg ccaggtctat cgcgtactca agaacgatcg attgattggc
     1081 attttgcctt tctttcactc attcggctac acgatgtttt ggttcgcggc caattcaggg
     1141 atcgggaccg cctgccatcc cagcccgctc gacgcggccg tgatcggctc gttggttgag
     1201 cggtaccgag tgactgtgct gctcgccact cccactttcc ttcaattgta catgcgtcgg
     1261 tgtacgccgg cgcagtttgg ctcgctgcgc ctggtcctgg ccggagcgga gaagctgccg
     1321 gaatcgatgg cgctggcttt cgaggacact tttggaatcc ggccgatgga aggctatggc
     1381 ctgaccgagt gttcgccggt agtggccgtc aacacgttcg actggcggga acctggcttc
     1441 tttcagcccg gctcgcggcg gggctacgtg ggccagccgc tccctggcgt atcggtgcgg
     1501 atcgtttcga gtgaaaccta cgagccgctc ggaccggatt gtcccggcct ggtactggtc
     1561 aagggcccga acgtgatgca gggctatctg ggccgcgacg accttacgaa ggctgccttc
     1621 catgaaggct ggtatgtaac gggggaccaa ggtttcgtca gtgaggatgg attcttgaag
     1681 atcaccggac ggctctcgcg gttttccaaa atcggcggcg agatggttcc gcacgggctc
     1741 atcgaagagc tgctgcagca gggagtcgcg gccgacgaac aggtcttcgc cgtcacggcc
     1801 gtaggcgacg agcgcaaggg agaaaagctg gtcgtcctgc acaccttgga cgacgagaaa
     1861 gtcgaccaag ccctggagcg cctgggatcg cacggtctgc cgaatctgtt tatcccgcgc
     1921 cgcgatcaat tcatcaaagt cgacaagctg ccgatgctgg gcactggcaa acttgacttg
     1981 cgggggatgc gacgagtcgc agaagaggcg atgaaaggca gggaagcggt gagcgtgtag
     2041 tcttccgcct gccgtggtag gacgcatcag caaagagcga tcttaaacat caagattgag
     2101 cttagagcgg gctaggctag gcgtgcgttg tcatacgaga gcaaggcgaa cttcgaccgg
     2161 atggacgcga ctctctggat cgagaagaat ggcatctttt ggacagacgg ttgtccggaa
     2221 gctggacatc gggctctcgt ttgtag
//
LOCUS       DATAQL010001389         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_902918, whole genome shotgun sequence.
ACCESSION   DATAQL010001389 DATAQL010000000
VERSION     DATAQL010001389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902918"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1105)
                     /locus_tag="VHS97_23865"
     CDS             complement(239..1105)
                     /locus_tag="VHS97_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451312.1"
                     /translation="MFAIVTIALLFLLMMMPRGREQARLLACQKNLGQIGKALALYHQ
                     TEHQLPAIVALAGIDQPNQTTSSGPLRILLDTLQLPDLTGLADLKSPPKALPGQVPGE
                     IPVPGFVCGSDPNATAGLFRAPISYRATTGDSPAGDNGLFALGRVIKLEDVEAADGLS
                     FTAAFSERMVGDNQSGHPFLGNYRAVPGPLSGPSCSADAGDSPWRGDAGSSWNWADYR
                     STLYNHALVPNQRPSCLALDGQTAFMGASSGHVRGVNLLKVDGSVSLVTPAIDPKIWK
                     EFARISPPAKPE"
     gene            complement(1447..>2330)
                     /locus_tag="VHS97_23870"
     CDS             complement(1447..>2330)
                     /locus_tag="VHS97_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451313.1"
                     /translation="SEVHDTKLVSRFGSLSWRADLPPRTAIAFETRTGNVGEPDETWS
                     PWSATQSNPASGATVAPLARFIQYRAKLTTADPRRTPDLRSVSLSYRTSNLAPEISRL
                     DVPDLSTADGAARQTRLNLRWDASDPNDDELSFTLKARKDGWPEWIDLTDEPITEKTF
                     AWDTTAFPSGSYRLKLVASDRLSNSPDDVLTRERESLSFIVDHDSPAVTVTARKSGAD
                     IMLKDEQTRLVKADYALDGGRWVPIFPDDGLFDTVQEKITLALPDLKPGVHLVMVRAT
                     DSAGNVGAGDALIEVKK"
BASE COUNT          471 a          719 c          689 g          451 t
ORIGIN      
        1 tagcgaattc ctcgggagcc acccggccac tctcgaattg agcgaggaga ccagcaaaac
       61 cgccgccgga gatccgctgg cgaaacactt cgccggattc gcctatacgg gcgcctaacc
      121 gttcgcaagc cttcccatag tcgaagaaag cgactacatt tccgaaatcg aatatcaaag
      181 catgttcgcg catgatcgct ccctgattac ttttgttgca ggcaccatcg cgcttttttc
      241 actccggttt ggccggtggg ctgatcctgg cgaactcttt ccagatcttg ggatcgatcg
      301 ccggcgtgac caggctgacg ctgccgtcga ctttaagcag gttgacgccg cggacatgtc
      361 cgctcgatgc acccatgaaa gccgtctggc cgtccaaggc cagacaggat ggtctctggt
      421 tcgggaccaa ggcatggttg taaagtgtcg agcgatagtc ggcccagttc cacgacgagc
      481 cggcatcacc gcgccatggg gaatctcctg cgtccgcgga acaactcggg ccggagaggg
      541 gcccgggaac cgctcgatag ttacccaaga acgggtggcc ggattgattg tcccccacca
      601 tgcgttcgga aaatgccgcg gtaaagctca ggccatcggc cgcctcaacg tcctctagct
      661 tgatcacgcg gcccaaagcg aacaggccgt tatcgcccgc aggcgagtcg ccggtcgtcg
      721 cacgataact gatcggggcc cggaacaagc cagccgtcgc gttgggatcg ctcccgcaga
      781 caaaaccggg gacgggaatc tcgcccggga cctgacccgg tagtgccttc ggcggtgatt
      841 ttagatcggc gagccctgtc aagtcgggca attgcaaagt atcgagtaaa atgcgcagcg
      901 ggcccgacga tgtcgtctgg tttggctgat cgatgccggc aagagcgacg atggccggaa
      961 gctgatgctc ggtttgatgg tacagcgcca gggctttgcc gatctgtccc aggttttttt
     1021 ggcaggccaa tagcctggct tgctctcgac cgcgtggcat catcatgagt aagaacaata
     1081 atgcgatggt gacgatcgcg aacacgacga tgacttcgac gagagtgacg ccgcgaaaac
     1141 gattgaagga gcactttccc ccccttgtga tgggggggcc agggggggtg gtcagtgagc
     1201 aatcaggcac aggtcttgca aagtttgcat taataaacca gcttaatctc atccatcgac
     1261 gttgatcatc ccatagttga tagcactgcg accactccaa ttcaagttga ccacaccgtg
     1321 gccgatcgct cgctgaccac ccccacctaa cccccccttc acaagagggg gaaagctgcc
     1381 ctcgacatcg tggcgcacaa taaacgcgac gaatacagca gcgttgccgc acggaccgcg
     1441 tccccattac ttcttgacct cgatgagcgc atctcccgcg ccgacgtttc cggccgaatc
     1501 ggtggcgcgc accatcacca agtgcactcc cggcttcaag tccggcagtg cgagcgtgat
     1561 tttctcctgg acagtatcga acaaaccgtc atcagggaaa atcggcaccc agcgacctcc
     1621 gtcgagcgcg taatcggcct tcaccaaccg cgtctgctcg tccttgagca tgatgtcggc
     1681 cccgctcttt cgagccgtga ccgtcaccgc cggcgagtcg tggtccacga tgaacgacaa
     1741 gctctcgcgc tcgcgcgtca ggacatcatc cggactgttc gagagtcgat cgctggccac
     1801 gagcttgagc cgatagctac ccgagggaaa cgcagtcgtg tcccaagcga atgtcttctc
     1861 cgtgatcggc tcatcggtga ggtcgatcca ctcgggccag ccatccttgc gagccttgag
     1921 cgtgaaactg agctcgtcgt cgttggggtc gctcgcatcc caccgcagat tcagccgcgt
     1981 ctggcgagcg gcgccatccg ccgtgctcag atcgggtaca tcgagccggc tgatctctgg
     2041 cgccaggttc gatgtacgat agctgagcga aaccgaccgc agatcggggg tacgccgcgg
     2101 gtcggcggtc gtcagcttgg ctcgatactg aatgaatcga gccagcggtg caaccgtcgc
     2161 tccactcgcc ggattgcttt gcgtggcgga ccaaggcgac caggtctcgt ccggttcacc
     2221 gacgttgccg gtccgtgtct cgaacgcgat cgccgtgcgc ggcggaaggt cggcgcgcca
     2281 ggagagcgat ccgaatcggc tcacaagctt ggtgtcgtgg acctcggata
//
LOCUS       DATAQL010001390         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1211885, whole genome shotgun sequence.
ACCESSION   DATAQL010001390 DATAQL010000000
VERSION     DATAQL010001390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211885"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1336)
                     /locus_tag="VHS97_23875"
     CDS             complement(176..1336)
                     /locus_tag="VHS97_23875"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HEX3451314.1"
                     /translation="MTCPHCHESARFVEYRPKTVQSLVGAFSLERGYYHCRSCGKGMV
                     PWDETLGLSPQALTAGARELVCIAGAVDSFGEAADVVLKKLAGLRVSESTVERTSEAV
                     GDDIGQRQAAGATFGAAAPWGWHKDAEGKTCAYVSLDLTGLGMQGPEGAAAEGRMAAV
                     GMIYNPLPEDPARWARPRGRAPQFQARYVAGLDGQASLAEPMRRQAAQVGMDQAQRWI
                     ALSDAGAGVEDLLRVNFGRVEAVILDFYHVAEHLGDLGRALYPADESTRKEWWERWCH
                     RLKHDGGQAVLDELRSLPADPRESVRKVHGEVVGYFENQVHRMDYPTYRAKGWAIGSG
                     PIESACKTVIGKRMKNGGMRWGEDGADGMCHLRALFASGEDQWDAYWHPSRN"
     gene            complement(1333..1575)
                     /locus_tag="VHS97_23880"
     CDS             complement(1333..1575)
                     /locus_tag="VHS97_23880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451315.1"
                     /translation="MSRLALSPQQAELSDRIFQTLRQAAEADLRSLADLLASKEDRQL
                     LGKTEFEVRDLVHRIGAKALETALDERKKGGTKGPA"
BASE COUNT          288 a          644 c          536 g          292 t
ORIGIN      
        1 gacccgagca cgggccggca gaccctggtc acccaggggg gcagcctcga gttcccgatc
       61 gcgctccagc ccgggccggg caacacgatc tatgtcgccg acttcgacac caactccatc
      121 tacctggccg acctccatac cgggtaggtc gaacttctgt gggtataccg tctgttcagt
      181 ttcgactggg atgccagtag gcgtcccact gatcttctcc gctggcgaat agggcgcgca
      241 ggtggcacat gccatcggct ccgtcttcgc cccagcgcat gcccccgttc ttcatccgct
      301 tgccgatgac cgtcttgcag gccgactcga tcgggccgga gccgatcgcc cagcccttgg
      361 cacgatacgt cggatagtcc atccgatgca cctgattctc gaagtaaccc accacctcgc
      421 cgtggacctt ccgcaccgac tcgcgagggt ccgccgggag cgagcgcaac tcgtccagga
      481 ccgcttgacc cccgtcgtgt ttcagccgat ggcaccaccg ctcccaccac tccttccggg
      541 tcgattcgtc cgccgggtag agcgcccggc ccaggtcgcc gagatgttcc gcgacgtggt
      601 agaagtcgag gatcaccgcc tcgacgcggc cgaagttcac ccgcagcagg tcctccaccc
      661 cggccccggc gtccgacagc gcgatccagc gctgggcctg gtccatgccc acctgggccg
      721 cctgcctccg catcggctcg gccagcgacg cttgaccatc cagtccggcc acgtaacgcg
      781 cctggaactg cggcgcgcgg ccccgcggcc gggcccaccg cgcgggatcc tccggcaacg
      841 ggttgtagat catcccgacc gccgccatgc ggccttcggc cgccgccccc tcggggccct
      901 gcatccccaa cccggtcaga tcgagcgaga cgtaggcaca ggtcttccct tcggcgtcct
      961 tgtgccaacc ccacggggcg gccgccccga aggtcgcgcc ggcggcctgt cgctgaccga
     1021 tgtcgtcgcc gaccgcctcg ctcgttcgct ccaccgtcga ttccgagacg cgcaacccgg
     1081 cgagcttctt gagcaccacg tcggcggcct cgccgaagct gtccaccgcg ccggcgatgc
     1141 agaccagttc gcgggccccc gcggtcagcg cctggggcga cagcccgagg gtctcatccc
     1201 aggggaccat ccccttgcca caggatcggc agtggtaata gccccgctcc agcgagaagg
     1261 cccccaccag gctctggacc gtcttgggcc gatactcgac gaagcgggcc gactcgtgac
     1321 aatgcgggca ggtcatgctg gacccttggt accccccttt ttccgctcgt ccagagcggt
     1381 ctcgagggcc ttggcaccga tgcggtggac gaggtcgcgg acctcgaatt cggtcttccc
     1441 caggagttgg cgatcctcct tggaggcgag caggtcggcg agggaccgaa ggtcggcctc
     1501 ggcggcttgg cgaagggttt ggaagatgcg gtcggagagt tcggcctgct gcggggacaa
     1561 cgcgagtcga gacatgggag ccctccgtga acagatgaca acgaaatccg agcagacgca
     1621 tcggagacct ctgttgtacg gaatttccca cccacaatcg agacgcctac ccccggaccc
     1681 gttccgtgcc tcataagaat tgacagatca tcgcccttgc tctaaaatac aacagtggga
     1741 tcggtccatc caggaggctg
//
LOCUS       DATAQL010001391         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1211887, whole genome shotgun sequence.
ACCESSION   DATAQL010001391 DATAQL010000000
VERSION     DATAQL010001391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211887"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1007)
                     /locus_tag="VHS97_23885"
     CDS             complement(57..1007)
                     /locus_tag="VHS97_23885"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEX3451316.1"
                     /translation="MTIGYDKPLYILPFDHRGTFQKNMFGWTGKLTTEQAATIAAMKC
                     LIYDGFQAALVGSVPEAKAGILVDEQFGTDILRDAAKRHFVTACPAEKSGQDEFDFEY
                     GNDFGHHIETFQPTFCKVLVRYNPEGDAAMNRRQSERLRRLSDYLHSVGLSRFMFELL
                     VPATPAQLERSAGDKKEYDRALRPELMIEAITELQEASIEPDVWKIEGIDRPEDCAKV
                     VATVRRGGRDRVSCIVLGRGEDDTKVRQWLKTAAAVPGFIGFAVGRTSFWEPLTDWKD
                     GRIARDAAVSEIARRYLEWVDIFESGRLPGRAAVAQGGGA"
     gene            complement(1040..1444)
                     /locus_tag="VHS97_23890"
     CDS             complement(1040..1444)
                     /locus_tag="VHS97_23890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451317.1"
                     /translation="MARRALAEGAILSLGSLWIDEERAHNFSLYARHADRVTLLLCGE
                     EDVARPVKEVVLFCLLFLANGIPIFAAGDEFMRTPGSIARSMSLIRDKKRRRPRSDTG
                     SWPDQWWSLWEERTVRDHPKDRSRGQFVLFRC"
     gene            complement(1500..2099)
                     /locus_tag="VHS97_23895"
     CDS             complement(1500..2099)
                     /locus_tag="VHS97_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451318.1"
                     /translation="MIVTKYTADDLKKLPLRAIATFAARCARRVEHQALLPDDHPEYE
                     KRRTAVAAAICLAEDFARDSLCTSFESVVRNVAESQTVAEGDFVRDIAMSAVVQAARA
                     AGAGLRVLQLRAEPEESHMFGAAKPNPFPHLADVTADLAAREAFTAAMEAAAATGHAD
                     DFINAGIADYEKLLQLDLGSYPQAGKPIDPSSTGPLGPL"
     gene            complement(2189..>2659)
                     /locus_tag="VHS97_23900"
     CDS             complement(2189..>2659)
                     /locus_tag="VHS97_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451319.1"
                     /translation="FIPSPDGKPYPNLAQHALREAKVLARNIHGVLNGQSPRPFVYNT
                     LGMMGSLGHSRAFGQLLKVRVHGVVAWFVRRTYYLLQMPGWSRRLRIMIDWTFAFLFR
                     PDIVKISLDSETVSLLREAAAGAVPENGRLAASHQSGEPSPVRGTVPGVGLGGT"
BASE COUNT          488 a          855 c          813 g          503 t
ORIGIN      
        1 ctgatcgagc gggtgcctgc gcgacttgat tgtcgggcta ttgtcgatac ggaccgtcat
       61 gccccacccc cttgggcaac cgccgccctg cccggtaagc ggcccgactc gaaaatgtcc
      121 acccactcca gatagcggcg agctatttcg ctgaccgcgg cgtcccgcgc gatccggccg
      181 tccttccagt cagtaagcgg ttcccagaag ctcgtccggc ccacggcgaa gccgatgaaa
      241 cctggcactg ccgcggcggt cttcagccat tggcggacct tggtgtcgtc ttcgccacga
      301 cccaagacga tgcagctcac acgatcgcgc ccgccgcgcc gcaccgtcgc gacaaccttg
      361 gcacagtcct cgggccggtc aatgccctca attttccaca cgtccggctc gatgctcgcc
      421 tcctgtagct cagtgatcgc ctctatcatc agctcgggcc ggagggcccg gtcatattcc
      481 ttcttgtcac cggcagatcg ctcgagttgt gccggcgttg ctggtaccag cagctcgaac
      541 atgaaccggc tgaggccgac ggagtgcagg taatccgata gccgcctcag ccgctccgac
      601 tggcgacggt tcatcgcggc atcgccctcg gggttgtagc gtaccaagac cttgcagaag
      661 gtcggctgaa acgtttcgat gtggtggccg aagtcgttgc cgtactcgaa gtcgaactcg
      721 tcctgcccac tcttctcggc cgggcacgcc gtgacgaaat ggcgtttcgc cgcgtcgcgg
      781 aggatgtcag tgccgaactg ctcgtccacg aggatgccag ctttcgcctc tgggacgctc
      841 ccgacgaggg ctgcctggaa gccgtcgtag atcaggcact tcatggcggc gatcgtcgcc
      901 gcctgctcgg tggtcagctt gcccgtccag ccgaacatgt tcttctggaa ggtccctcgg
      961 tgatcgaagg gcaggatgta gagcggtttg tcgtatccga tggtcatcac tatgagctcc
     1021 gttacgtttg agatggaatc taacagcgaa acaaaacaaa ctgaccgcgc gatcggtctt
     1081 ttgggtgatc acgtacggtc cgttcttccc acaaggacca ccactgatct ggccaggacc
     1141 cggtatcgct gcgagggcgc cggcgcttct tgtcccggat cagcgacatc gagcgggcta
     1201 ttgagcccgg cgtccgcatg aactcgtcgc cggcggcgaa tatcggaatg ccgttggcca
     1261 agaacaggag gcagaagagg accacctctt tcaccggccg ggccacgtcc tcctcgccgc
     1321 agagcaagag ggtcactcga tcggcatgcc gggcgtagag ggagaagtta tgggcgcgct
     1381 cctcgtcgat ccacagcgat cccagagaga ggatcgcccc ctcggcaaga gccctccttg
     1441 ccatcgcaga tcatttcatc gggattggga cagggtatta ccgctggaga ttcagcctgt
     1501 cagagcggcc cgagcggtcc cgtcgacgag gggtcgatcg gctttcctgc ctgcggatag
     1561 ctgccgaggt caagctgcaa gagtttctcg tagtcggcaa ttcccgcgtt gataaagtcg
     1621 tcggcgtgcc cggtggccgc ggccgcctcc atggccgcgg tgaaggcctc acgcgcagcc
     1681 aggtcagccg tgacatcggc gagatgcgga aaaggattgg gcttggcggc tccgaacatg
     1741 tgtgactcct ccggctcggc gcggagctgg aggacgcgca gaccagcccc agctgcgcgc
     1801 gcggcttgga cgaccgcgga catcgcgatg tcgcgcacga agtctccctc cgcgacagtc
     1861 tgactctccg cgacattgcg gacgacggac tcgaaggatg tacacagtga gtccctcgca
     1921 aaatcctcgg ccagacagat ggccgcagcc acggcggtcc gacgcttttc gtactccggg
     1981 tggtcatccg gcaggagagc ttgatgctcg actcggcgcg cgcaacgggc ggcgaaagtg
     2041 gcgatcgcgc gcagcggcaa tttcttcaaa tcgtcggcgg tatatttcgt aacgatcatc
     2101 ctcgtctctc tcggttgggg aacgaccatt tctgtaaatc accatagtct cacagcataa
     2161 tatctagtcg gcattcactt cgacccgctc acgtaccgcc cagtcccaca ccagggacag
     2221 tccctctcac cggtgacggc tcgcccgatt ggtggcttgc cgcgagtcga ccgttctcag
     2281 ggacagcacc tgccgcggct tcgcggagga gcgacaccgt ctcgctgtcc aggctgatct
     2341 tcacgatgtc cggccgaaac agaaacgcaa aggtccagtc gatcatgatg cggagccgcc
     2401 ggctccagcc cggcatctgg agcaggtagt acgtgcgtcg cacgaaccac gccacgaccc
     2461 catgcacccg cactttgagg agctggccaa acgcccggct gtgacccagg gaacccatca
     2521 tccccagcgt gttgtagacg aacggccggg gcgactgtcc attcaggacg ccatggatgt
     2581 tgcgggccag cactttggcc tctcgcaagg catgctgggc caggttcgga taaggtttgc
     2641 catcggggga tgggatgaa
//
LOCUS       DATAQL010001392         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3654397, whole genome shotgun sequence.
ACCESSION   DATAQL010001392 DATAQL010000000
VERSION     DATAQL010001392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3654397"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..461)
                     /locus_tag="VHS97_23905"
     CDS             complement(81..461)
                     /locus_tag="VHS97_23905"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX3451320.1"
                     /translation="MLFPGGFTFPNFLTDVFSIFIFVLWFWLLIVVFGDLFRRRDVSG
                     LGKVLWVIVLIVFPYIGVFVYLISQGRGMAERRNQQVQQARDELRQVVGFSVADELEK
                     LERLKNSNTISNEEYMRLRARVVQ"
     gene            complement(764..1270)
                     /locus_tag="VHS97_23910"
     CDS             complement(764..1270)
                     /locus_tag="VHS97_23910"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX3451321.1"
                     /translation="MSDLLVIEFLSEEKAEGVRETLLAMQKEYLIELGDAVVAVKDAN
                     GRIKLSQLFQPVAHGAAASGLFWGSLIGLLFMMPLAGAAIGAASGAISAHLTDLGIDD
                     EFMKQAARTLQSGNAALFLLIRKMTTDKVLAALHGAGGAVLRSSFDETKEEALRAALA
                     GAQAAAAG"
     gene            complement(1362..>1733)
                     /locus_tag="VHS97_23915"
     CDS             complement(1362..>1733)
                     /locus_tag="VHS97_23915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451322.1"
                     /translation="VGTSSEGDRIMTQYRKRVLMAACTAVTLIGGSVAHAQAAATPLE
                     QAELAGLSPEMRAQVQARAVGGNSVTEVLQVMLLNNIKIKHQASLIVALDWGKGVAVV
                     RLPSGGMAVVRFDPTTLQIKS"
BASE COUNT          364 a          564 c          483 g          322 t
ORIGIN      
        1 tagcaacggc gcgtcggtcg gacgacaccg atccgccgca gacctgatca caaagcggtg
       61 cgcccaagct ccagtcccgg ctattgcacg actcgggcgc gcagccgcat gtattcctcg
      121 ttcgagatcg tgttcgaatt cttcagccgt tcgagtttct cgagttcgtc ggccacgctg
      181 aagccgacta cttggcgcag ttcgtcgcga gcttgctgga cttgctgatt gcgtcgctcc
      241 gccatcccgc gaccctgcga gatcaggtag acaaacaccc cgatataagg gaacacaatc
      301 agcacgatca cccaaagcac tttgcctaag ccggacacgt cccgccggcg gaacaggtcg
      361 ccgaagacca cgatcagcaa ccagaaccac aacacgaaga taaagatcga aaagacgtcg
      421 gtcagaaagt taggaaaagt gaaaccacct ggaaacaaca tgtcagcctc cccttttgat
      481 ggcttgcacc gcccgccgag cgggtgaaca tcacgccgac cgaagtaatc gtggtccgat
      541 ttccggcctg tggcagaggc agccggcatc accggccgac gcggctatat cacccgacct
      601 gtgagagacc acggctctca ccttcaagca atctcgaacc gacattctcc ggatagttct
      661 gtgccccgcc ggacttgagc atggataacg caatggaccc gatcaagagc gtcagcgtgc
      721 tccaaggcgg gcgtgagatc cggacagccc atcggctcaa agctcaacct gcggctgccg
      781 cctgagcgcc cgcaagggcg gctcgcaagg cttcttcctt ggtctcgtca aacgaactcc
      841 gcaaaactgc gccgccggcc ccgtgcagcg ctgcaagcac cttgtcggtc gtcattttgc
      901 ggatcagcaa gaacagggcg gcgttgccgg attgcaaagt tctcgccgct tgtttcatga
      961 actcatcgtc gatgccgaga tcggtcaaat gtgcgctgat cgcaccggat gcggccccga
     1021 tcgcagcgcc cgccagcggc atcatgaata gcaacccgat cagcgaaccc cagaacaaac
     1081 ccgacgcagc ggcgccgtgt gcgactggtt gaaagagttg gctcaacttg atgcggccgt
     1141 tggcgtcctt caccgcgacc acagcgtctc ccagctcgat caaatattcc ttttgcatgg
     1201 cgaggagcgt ctcacgaacg ccttcggcct tttcttcgct cagaaactcg ataacgagca
     1261 gatcagacaa cgggaatcct ccactcgccg cgaccggaca ggatttgagc aggggcgcgt
     1321 tgcaccggcc gcggcgcgct cctgtccggt tgctcggatg cctaactctt gatttgcaac
     1381 gtggtcgggt cgaaacggac gaccgccatg ccaccgctcg gcagccgcac gacagctacg
     1441 ccttttcccc aatccagggc gacgatcagg ctggcctgat gcttgatctt gatattgttt
     1501 agaagcatca cctgcaggac ttccgtgacc gagttgccgc cgacagctct ggcttgaacc
     1561 tgggcgcgca tctccgggct gaggccggct agctcggcct gctccagcgg tgtcgcggcg
     1621 gcctgggcat gcgccaccga gccgccaatc agcgtgacgg ccgtacacgc ggccatcaac
     1681 acgcgtttgc gatattgtgt catgatgcga tctccttcgg acgatgttcc cac
//
LOCUS       DATAQL010001393         3531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3959795, whole genome shotgun sequence.
ACCESSION   DATAQL010001393 DATAQL010000000
VERSION     DATAQL010001393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3959795"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1510
                     /locus_tag="VHS97_23920"
     CDS             179..1510
                     /locus_tag="VHS97_23920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451323.1"
                     /translation="MATGLRPPFALRPMIADPVEPTRTEVGLALAIAILFVVATRWPV
                     ARIAPFEFDEFSFLGQTAVHWFPMHHTLFMTFGRVLGLVAGEPYRGFILLDMLVSALA
                     LVSVWWMLRAIVRPATAAAATLVLGVGPVFWSYGAMAGNYPAIIVVGSLLLGIAYRGL
                     SSPRAWQPLAASVVLAIGTGYRQDIGIFWLPVFGVILWQHRWKPAIAAGLLFTVLNAA
                     WLSAMLLEVGGWTHYRAASAEFAHEAGYLNSVWNLGFTDGPLRYAVKLGMALVGTLGP
                     ALFFVPRGAARLGRVDGGWFLGSLMVLSALPALGSHLLIHFGVAGYSFHYVPALLALL
                     ALGVSRSSATDCSALESFPDSPARDLVGTATPRLIAIALVLAGLFWFYPTDYAQRGWR
                     GSFDLSFCRFTRIGLKTPLPDHAPEYWRTANSRPLAGTPVRRPADSRAGAG"
     gene            complement(1580..2464)
                     /locus_tag="VHS97_23925"
     CDS             complement(1580..2464)
                     /locus_tag="VHS97_23925"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX3451324.1"
                     /translation="MVNYISGLVFVVLLLLGIVLAAAIGQTSPVTAVIFSLAWLFLDV
                     VVSSSIRLAAQWQKAVVFRLGKFHSIKGPGLFMIIPLIDQLRMVDTRVLAVNIPKQQV
                     ITRDNVPVTIDGVLFFRVDNPAEAIIMVQDYRYMIAQYSQTSLRDVIGQMSLDQLLTE
                     REEIAKAIEQHVEKDTKGWGLEVTGLRIQDIDMPEELKKMMSRQASAEREKRATITKA
                     EGDKEAAVNLSLAAKTMAASPGAMQLRTLQTIDGLGPTASNTVVLAVPIEILESLALL
                     KEAYRNNAAAERSAAPSA"
     gene            complement(2601..>3531)
                     /locus_tag="VHS97_23930"
     CDS             complement(2601..>3531)
                     /locus_tag="VHS97_23930"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX3451325.1"
                     /translation="AQLDRTEASARLKMATAEVKEKQGLLKSPATSQSLSPDVIEAQI
                     EAAQAKLELAQLALDRCTLRAPFAGRLVALPVCAGQYVLKGTTVAELADLSSLKTLLP
                     VDRREVAAGAALTVQIEGRDVAGKVQAILPLPERYTALRELATPFATAWVVVPNTKGD
                     LEPGLRVRPMTIPVAPIATVAKRAVKQEDPRKNEGTMVQVIRNEYVVNVPVRVLGETG
                     PERVQITGLLRRSDSLIVSSSVALLHGTLVRFGEGTSSRAVEGVAPNPAFGGAEAGIT
                     APGGTRARGAISGSTPNQTKPATEAPKTGSAPF"
BASE COUNT          582 a         1093 c         1104 g          752 t
ORIGIN      
        1 taccccccat ctccgaaaaa gttcatgtat gaccaagctg agcgtttcga cgcacatatc
       61 cagattgacg aggaatttgt cgccgtgctc gacgtagcgg atcgcggctt gcgatactcg
      121 ctattttcca ttattgttac cccatcatga cgatcccctg ggaaggagtc ccgacctaat
      181 ggcaacagga ctgcgcccgc cattcgctct gcgaccaatg atcgccgatc cggtcgagcc
      241 cacccgaaca gaggtgggcc tggcgctggc aatcgcaatc ctctttgtcg tcgccacgcg
      301 ctggccggtc gcgcggatcg cgccttttga attcgatgaa ttctcgttcc tggggcagac
      361 ggcggtgcac tggtttccca tgcaccatac gctcttcatg acgtttggcc gcgtcttggg
      421 gctggtcgcc ggagagcctt accgcggttt catccttctc gatatgctgg tgagcgctct
      481 agctctcgta agcgtgtggt ggatgcttcg cgcgatcgtc aggccggcga ccgcggctgc
      541 ggcgacgctc gtcctgggcg tgggtcccgt gttttggagc tatggtgcca tggcgggtaa
      601 ctacccggcc atcatcgtgg tcggcagctt gttgctcggc atcgcctacc gtggcttgtc
      661 ctcacccaga gcctggcagc cgttggcggc gtccgtcgtg ttggcgatcg ggacgggcta
      721 ccgacaggat atcggaatat tctggctgcc agttttcggc gtgatcctct ggcaacaccg
      781 ctggaagccg gcgatcgctg cgggtctact tttcacggtg ctcaacgcgg cgtggttgtc
      841 ggctatgctc ttggaggtcg gcggctggac ccattaccgc gccgcgagcg ccgagttcgc
      901 ccatgaggcc ggttatctga actcggtctg gaacctgggg tttaccgacg gcccgctccg
      961 atacgcggtc aagctgggga tggccctggt cgggacgctt ggtccggctc tcttcttcgt
     1021 tccgcgcggg gcggcgcggc tgggtcgcgt agacggcggc tggtttctcg gctcgttaat
     1081 ggtgctgtcc gccttgccgg cgctgggatc tcatctcttg atacattttg gagttgccgg
     1141 ctatagcttt cattacgttc cggccctgtt ggcgctcttg gcactcggtg tgagccgatc
     1201 gtcagcgacg gactgctctg ccctggaatc gtttcctgat tcgccagctc gcgatttagt
     1261 ggggactgcg acaccgcggc taattgcgat cgcgcttgtc ctggcgggcc tgttctggtt
     1321 ttacccgacc gactacgcgc agcgaggctg gcgcggcagc ttcgacttgt cgttttgccg
     1381 gttcacgcgg attggactca aaacgccgct gccggaccac gcgccggagt actggcggac
     1441 cgccaattcg cgtccactag cgggcacccc ggttcgccgg cccgctgatt cgcgggccgg
     1501 cgccggatag atcgcgcgta tcggctgcgc accaaatcgt cgctaatggc aaggaatgtc
     1561 cttgccatta gccattgcct caggcggacg gggcagccga ccgttcggcg gccgcgttgt
     1621 tgcgataggc ttccttgagc aaagccaggc tctcgaggat ttcgatcggc acggccagga
     1681 cgaccgtgtt ggaggccgtc gggcccagcc cgtcgatcgt ttgcagcgtt ctgagctgca
     1741 tcgccccagg actggccgcc atcgtcttgg ccgccagcga caggttgacc gcggcttcct
     1801 tgtcaccttc ggccttggtg atcgtggctc gcttttcccg ctcagcggaa gcctgccgcg
     1861 acatcatctt cttcagctct tcaggcatat cgatatcttg aattcgcagc ccggtgactt
     1921 ccaggcccca gcccttggtg tctttctcga catgttgctc gatcgccttg gcgatctctt
     1981 cgcgctcggt gagcagctgg tcgagcgaca tctggccgat gacgtcacgc aacgaggttt
     2041 gcgaatattg ggcgatcatg tagcgatagt cctgaaccat gatgatggct tccgccggat
     2101 tatccacccg gaagaagagc actccatcaa tggtcaccgg gacgttgtcg cgcgtgatca
     2161 cctgctgctt gggaatgttt actgccagca cgcgcgtatc caccatccga agctggtcga
     2221 tcaagggaat aatcatgaag agccctgggc ccttgatcga gtgaaacttg cccaggcgaa
     2281 acacgaccgc cttctgccac tgggcggcca ggcggatcga cgacgagacg acgacgtcga
     2341 ggaacagcca tgccaggctg aagataacag ccgtgacggg gctagtctgg ccgatcgcgg
     2401 ccgccagcac gatccccaag aggagcagca cgacgaacac gagacccgag atgtaattga
     2461 ccatcggaat tgcccttacg ctttcaatgt cggtgccgga cggacgacgt ccggagtgag
     2521 cggctgcccg ggacacccgt gccgcgtgct cactgatcca ttcgcgtggt gcaatcggtt
     2581 attgatgtga atcggacggt ctaaaacgga gcggaccccg ttttgggggc ctcggtcgcc
     2641 ggcttggttt gattgggggt cgaccccgat atcgcgccac gtgcgcgcgt gccgccgggc
     2701 gcagtgatgc cggcctcggc accaccgaat gcgggattgg gagccacccc ctcgaccgcc
     2761 cgcgaactgg tcccctcgcc aaagcgaacc aaggttccat gcaagagcgc gaccgacgac
     2821 gacacgatca gtgagtccga ccggcggagc aacccggtga tttgaacgcg ctccgggcca
     2881 gtttcgccca ggacgcgcac tggcacgttg acaacgtatt cgttgcggat cacttgaacc
     2941 atcgttcctt cgtttttccg aggatcctct tgtttgacgg cgcgtttggc gaccgtggcg
     3001 atcggtgcga cggggatcgt catcggtcgt acgcggaggc ccggctccaa atctcccttg
     3061 gtattgggaa caacaaccca tgccgtggca aagggcgtcg ccagctcgcg tagagccgtg
     3121 tagcgctcgg gcaggggtaa gatcgcctgg accttacccg cgacatctcg cccctcgatt
     3181 tgtacggtca gggcagcccc ggctgcaact tcgcggcgat cgacgggcaa gagcgtcttc
     3241 aggctggaga gatcggccaa ctctgcaacc gtcgtccctt tgaggacgta ttgtccggcg
     3301 cacaccggca aggcgaccag ccgccccgca aacggcgccc gcagtgtgca gcgatcgaga
     3361 gcgagctgag ccagctcgag cttcgcctgc gcagcctcga tttgcgcttc gatgacatcc
     3421 ggtgaaaggg attgagacgt cgccgggctc ttcaagagac cctgcttttc tttgacctcg
     3481 gcagtggcca tcttcagtcg agcggacgcc tcggtgcggt cgagctgggc g
//
LOCUS       DATAQL010001394         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_753784, whole genome shotgun sequence.
ACCESSION   DATAQL010001394 DATAQL010000000
VERSION     DATAQL010001394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753784"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..318)
                     /locus_tag="VHS97_23935"
     CDS             complement(157..318)
                     /locus_tag="VHS97_23935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451326.1"
                     /translation="MTKRLANCRSRMEKLGAGRLPGRFFAASRAKLWEIGECLGVWYM
                     VNLAGCPMR"
     gene            complement(373..1707)
                     /locus_tag="VHS97_23940"
     CDS             complement(373..1707)
                     /locus_tag="VHS97_23940"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX3451327.1"
                     /translation="MSWTQEQARALSDKILAMSKAPACAVTLRRQTLGHTRFAANDVT
                     TAGISENVSITIESIDGARSATTTADTTDEAGLRAAVARNEAMLATARPNPEAVEPLG
                     PQEYPVIAAFDESTAHAGATVRSQGVKAALDLARGRDLNASGFFETGATWSAIANSKG
                     NFGFHRSTSAGYSATMRTQDGTGSGYAALEAPRLAGVDAAALAQRAAHKAENSAKPRD
                     LAPGEYPVILEPQAVSDLLSGIVMAFNARAAEEGRSYFSKPGGGTRLGEKVFADCVML
                     RSDPTDPRVPGAPWAVGGLPVHKTTWIENGIVRNLTCDRYWAKKSGREPLPFSGGLVV
                     EGGSGSLDELIAQTERALLVTRFWYIRSVNPREGLVTGLTRDGVWLIEKGKIAYPVNN
                     FRFNDGPIRLLQNVDAMSAPYPTGSGGSFFSAAMLPAIRARAILFTSKSDAI"
     gene            complement(1709..>3212)
                     /locus_tag="VHS97_23945"
     CDS             complement(1709..>3212)
                     /locus_tag="VHS97_23945"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX3451328.1"
                     /translation="GAAAAEMGEDERAKLAATALEIARSQGASYADIRINRYRNQSIS
                     FRAQASPGERGPVEVPGVADSESFGFGIRVLAEGTWGFASSYDVSNDAIARATARAVE
                     IARANAPLRRQPVQLAPTPAYKDSYRTAIAVDPFSVPIAQKLDLLRAVAIETLAVKGV
                     FSVTAFIRQRSEDRLFASSDGSMIHQVIYQVGPEFTAQAVDAGRKLKSRTYRPNAVCA
                     WYEAVSRAKLVENASRVGAQAVEHLKAPSVSPGVKDLILLPTHLGLTIHESIGHSTEL
                     DRALGYEANYAGTSFLTPDKLGKFRVGSEIVNVFGDRTRPESLSTCGYDDDGVKTRQF
                     PIIKDGIFVGFQTIRDQAHLIGQTESMACCYADSYDSIPFQRMPNVWLAPSSQATTLD
                     DLISGVDDGILIDGRGSYSIDQQRYNFQFGGDAFFAIKGGKLGPMIADVAYQSRTPDF
                     WASCDGIGGEALWENVGLTNDGKGQPGQVNAMSHGCAPARFRRINVLRTA"
BASE COUNT          581 a         1010 c         1014 g          607 t
ORIGIN      
        1 ggtcaggttc aattgttcct gattcccttc gggcttcgat tcagtggact tccgccagga
       61 cgttatggga tatgcctgac gacgtgcagt acctccctgc cgcggcaagg cgttcgaacg
      121 ccgacggttc gacgcgatct agattttaga atcgtgtcat cgcatcggac aacccgccag
      181 attgaccatg taccacacac cgaggcattc gccaatttcc cacagcttcg cgcggctggc
      241 cgcaaagaag cggcccggca accgacccgc gccgagcttc tccatccggc tccggcaatt
      301 cgccaggcgt ttggtcacgg cgagcaactc gtcgattcgc ccacttgatc ggacgtgaaa
      361 tggcgacaat cgttagatcg cgtcactttt ggaggtgaaa agaatcgcgc gagcgcgtat
      421 cgccgggagc atcgcggcgc taaagaacga tccgcctgaa ccggtcggat atggcgcgct
      481 catcgcgtcg acgttctgga ggaggcgaat cggaccgtcg ttaaatcgga agttgttgac
      541 ggggtaggcg atcttgccct tctcaatcag ccagactccg tcacgagtca gacctgtgac
      601 gagaccctcg cgcgggttga ccgaacggat gtaccagaat cgggtgacga gcaaggctcg
      661 ctccgtttgg gcgatcagtt catcgagcga gcctgaaccc ccctccacga cgaggccgcc
      721 cgagaacggg agcggctcgc gcccagactt tttggcccag tagcgatcgc aagttaagtt
      781 gcgcacaatc ccattttcga tccacgtcgt cttgtggacg ggcaagcctc caaccgccca
      841 gggtgcgccg ggcacgcgcg ggtcggtggg gtctgatcgc agcattacgc agtctgcaaa
      901 aaccttttcg ccgagacgcg tgccaccccc gggtttcgag aagtagctgc ggccctcctc
      961 ggccgcccgg gcgttaaacg ccatgacgat tccgctcagc aagtcggaga cggcctgggg
     1021 ctcgaggatc acggggtatt cgcccggtgc caggtcgcgg ggttttgccg aattctcggc
     1081 cttgtgcgcc gcccgctggg ccaatgcggc ggcgtcgacg cccgccagac gcggagcctc
     1141 gagcgcggca tatccggagc cagtgccgtc ttgcgttcgc atcgtcgcgg aatagccggc
     1201 cgaggtcgaa cggtggaatc cgaaattacc cttggaattg gcgatcgccg accaggtcgc
     1261 tccggtctca aaaaaccctg acgcgttcag atctcggccc cgcgccaggt cgagcgcagc
     1321 cttcacgccc tggctacgga cggttgcgcc ggcatgggcc gtactttcgt caaaggccgc
     1381 aatgaccgga tattcttggg gaccgagcgg ctcgaccgct tccgggttcg gacgcgccgt
     1441 cgccagcatg gcttcgttgc gggcgaccgc tgcacgcagg ccggcctcat ccgtcgtgtc
     1501 ggccgtggtc gtggccgacc gtgccccgtc gatgctctcg atcgtgatcg acacgttttc
     1561 tgatattccc gccgtggtga catcattggc ggcaaatcgg gtatggccga gcgtctgccg
     1621 gcggagcgta accgcgcacg caggtgcttt gctcatcgcc aaaatcttgt cggacagggc
     1681 gcgggcttgc tcttgggtcc agctcatctc aggcggtcct caacacgttg attctgcgga
     1741 atcgagccgg agcgcacccg tggctcatgg cgttgacttg ccccggctgc cctttgccat
     1801 cgttggtcag accgacgttc tcccagagag cctcgccacc gatcccgtcg cacgaggccc
     1861 agaagtcggg cgtccggctc tggtaggcga catcggcgat catcggtccc agcttccctc
     1921 ccttgattgc gaaaaaggca tcgccgccga actgaaagtt gtaccgctgc tggtcgatcg
     1981 agtacgaccc acgaccgtca atcagaatgc catcgtccac gccggagatc aggtcatcga
     2041 gcgtggttgc ctgggacgac ggagcaagcc aaacgttggg catgcgctga aacgggatcg
     2101 aatcatacga gtcggcatag cagcaagcca tcgactcagt ttgaccgatc aggtgggcct
     2161 ggtcgcggat cgtctggaag ccgacgaaga tgccgtcctt gatgatcgga aattgccgcg
     2221 tcttgacccc gtcgtcatcg taaccacagg tcgaaagact ctccggccgg gtccgatcgc
     2281 cgaagacgtt gacgatctcg gatccgacgc ggaacttgcc aagtttgtca ggagtcagaa
     2341 agctggtgcc ggcgtagttg gcctcgtaac ccaacgcacg gtccagctcg gttgagtggc
     2401 cgatcgattc atggatcgtc aatcccaggt gggtcgggag caagatcaga tccttgacgc
     2461 cgggtgatac cgacggggcc ttcaggtgct cgacagcctg ggcgccgacg cggctggcat
     2521 tttcgaccag cttggctcgc gacacggcct cataccacgc gcatacggcg ttgggccggt
     2581 aggtgcgcga cttgagctta cggccggcat cgacagcctg ggccgtgaac tcggggccga
     2641 cctgatagat cacctggtgg atcatactcc cgtcgctcga cgcgaatagt cgatcttcgc
     2701 tccgctggcg gatgaaggcc gtgaccgaga agaccccctt gaccgccagg gtttcgatcg
     2761 cgaccgcgcg gagaagatcc agtttctggg cgatcggcac cgagaacgga tcgacggcga
     2821 tggcggtacg atacgagtct ttgtatgcgg gtgtcggagc tagttgtacg ggctggcgcc
     2881 gcaagggcgc gttcgccctg gcgatctcga cggcccgggc agtggcacgg gcaatcgcat
     2941 cattgctcac atcgtacgac gaggcaaagc cccacgtccc ttccgccagc acgcggatgc
     3001 cgaagccaaa gctctcggaa tcggccacgc cgggcacttc gaccgggccc cgttcgcccg
     3061 ggctagcctg tgcccggaag ctgatggact ggttgcgata gcgattgatc cggatgtcgg
     3121 cgtaagacgc accctgtgag cgggcgatct ccagggcagt cgcagcgagc ttggcgcgct
     3181 cgtcctcgcc catctcggct gcggccgcgc cg
//
LOCUS       DATAQL010001395         4974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4415398, whole genome shotgun sequence.
ACCESSION   DATAQL010001395 DATAQL010000000
VERSION     DATAQL010001395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415398"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            614..1348
                     /locus_tag="VHS97_23950"
     CDS             614..1348
                     /locus_tag="VHS97_23950"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX3451329.1"
                     /translation="MVASSRGILAIGLGLTGLAILVAPTLGQPQQKDGAVRKAATPTS
                     STKWPTPIAPVIGTVDLELVFKSYHKVAQSNKEYSAALMARKNELMKIMSEAQEESQM
                     LSKLAPGTEDYRKHENKVTQLKAQHEAGREQAEREFALRQAEAMATLYKEIQEMVKML
                     AKERNMNYVVKVSSQPITGTDPNSVMAAISSTMVYADPRNDLTNDVIHNLNRFYKATA
                     VPAAKSAAGAGSAPGLPANAPGTDGN"
     gene            1529..2353
                     /locus_tag="VHS97_23955"
                     /pseudo
     CDS             1529..2353
                     /locus_tag="VHS97_23955"
                     /inference="COORDINATES: protein motif:HMM:NF015299.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
     gene            2348..2920
                     /gene="fabZ"
                     /locus_tag="VHS97_23960"
     CDS             2348..2920
                     /gene="fabZ"
                     /locus_tag="VHS97_23960"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX3451330.1"
                     /translation="MARRLLRHAAQSTAPRGLESTSHDHGSLDIHAIMNLLPHRYPFL
                     LVDRVLELEPGHRALAIKNVSANEPYFRGHWPGQAIMPGVLIVEALAQAAGILVAKSV
                     ERRPGWVALIASINGVKLRRPVVPGDQLRLEVFGQKIKSNSACVSGQAKVGDALAAEA
                     KLRFVMVDAERAARSFDSNALLEKVPTRVG"
     gene            2986..3792
                     /gene="lpxA"
                     /locus_tag="VHS97_23965"
     CDS             2986..3792
                     /gene="lpxA"
                     /locus_tag="VHS97_23965"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX3451331.1"
                     /translation="MATLIADTACIDPRADVGDDVEIGPYCVVGPDVTIGRGTRLIAH
                     ACIQGTTILGEENVVHPFAVIGGEPQDVSFRGSQTCLEIGDRNVIREGVTIHRGSEKE
                     QGVTRIGSNNLLMVNVHVAHDCVIDDRVIIANNTILGGHGHVESYVTISGGVGIHPFV
                     SIGSYSYVGALSRIYHDVPRFMIIDGNPSKVRCINVVGLRRHGISPEAIAALHEAHRL
                     IYRARMAVSHASEILESHGHMCPEVKSLLEFIEEQHLGKHGRARERRRSA"
     gene            3789..4898
                     /locus_tag="VHS97_23970"
     CDS             3789..4898
                     /locus_tag="VHS97_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3451332.1"
                     /translation="MKRLRVGVVGVGHLGQHHARIFSSLAGVELVAIADARPEQAHAV
                     AARCGTVALCDYRPLLDIVDAVSIAVPTALHREVAGAFLEKGIPTLVEKPMAGTLLES
                     EQLVALARARDTLLQVGHIERFNPALRALDKLPVRPKFVNAERLSTYTFRSTDIGVVL
                     DLMIHDIDLVLSLIPAPVRSLAAVGVSLFGEHEDVANARIEFEDGSVANLTASRASYS
                     PSRKMRIWGAEGYASLDFAAKQATLIRPSEQLKGGRLDLEGVDLSQAAAVREHLFGKV
                     LRVDRVQEEGREPLSLELEDFVSAARGLSRPRVSGEDALRAVRLADQILRSLDAHRWD
                     AETGPAAAQTVESVSILQGPHAWRTRSMRQVSNRA"
BASE COUNT          962 a         1502 c         1534 g          976 t
ORIGIN      
        1 gcggaccgaa cacttcggcg agctgctcct ctcgctgttc tcagcggccg gcgaggttcc
       61 ccgcaacaag aaatatcgct ttcacgattt cgtcttggcc tactccggag agtgaaagtc
      121 ggagttcgca cagcctgaat atcgacccgc ttttttccct tcgggaatcc cgtggctttt
      181 ccggttagag ctgggctgtc acgggattcc gttatttgta tcgctctgca gccgacactt
      241 gatgcttgca tgtatttggc atcgaccgtc acgcgaatat acggcaggtc tcggcttgcg
      301 cggtcggtta gagcgttgta aacgcgccag ttcggcttga cccatccact tcctgagcgc
      361 aaaatgccgc gcgttgccat ccggcgaaca ctttcagctc gggcgaacgg ggttttctct
      421 ccgatcgtag atcatcgctc cgactgaaag atccaccccg cgggtgcatc gagacgacaa
      481 ccgcctttcg cattcaagat cgcccggtca ggcagccggc ttgatctcgt gagtgcgtgg
      541 ctgtcgagcc ttggactaaa aagagtgact tcattacgaa tcggattgcc tcggtagaaa
      601 aggagttgta ccgatggtcg cttcatctcg cgggatcttg gcaatcgggc taggcctgac
      661 aggtttggca attctggtcg ctccaacgct gggtcaaccc caacagaagg atggtgcggt
      721 ccgcaaagcc gcaaccccaa cgtccagcac caaatggccc acgccgatcg ccccggttat
      781 cggcaccgtc gacctcgagc tcgtgttcaa gagctaccac aaggtcgcgc aatcgaacaa
      841 ggaatatagc gcggccctta tggcccggaa gaatgagctg atgaagatca tgtccgaggc
      901 tcaggaagaa tcgcagatgc tgtcgaaact ggcgcctggc accgaagact accgaaagca
      961 cgaaaacaag gtgacccagc tcaaggcaca gcatgaggcc ggccgcgagc aggcagaacg
     1021 cgagtttgcg ttgcggcagg ccgaagcgat ggccacgctc tacaaggaaa tccaggaaat
     1081 ggtgaagatg ctcgccaagg aacgcaacat gaactacgtc gtcaaagtgt cgagccagcc
     1141 gatcacgggg actgatccca attcggtgat ggcggccatc tcgagcacta tggtgtatgc
     1201 cgacccgcgg aacgatctca ccaacgacgt gatccataac ctcaatcggt tctacaaggc
     1261 gaccgcggtt cccgcggcca agtccgccgc cggtgcgggc tctgcccctg gcctcccggc
     1321 taatgctcct gggaccgacg gtaactaagc gatccgtggt cggtccgcat tcaagaccgg
     1381 cgccatcggg cgccggccgc ggactgacag cacagaaggg gccggttcca tcacgcgaat
     1441 cggccgcttc atgggtaagt gagcggtcga accgtctccg acgccggacg ctagggccat
     1501 gtcgtaggat gacctgaagg ggcaaggaat gctcagagct acgcgtccag agcgcaccat
     1561 tgcccgcacg acggaagtgc gcgggtttgg cttctttcac ggcgctgatg tgacgcttcg
     1621 attcaacccg gccgatgcgg gcacgggcgt ggtctttgta cgcacggact tgccggaccg
     1681 accggccgtg ccggcccgga ccgaaatggt gatcccgtca cagcgccgca cgaccatacg
     1741 caagggcgcc gcgtcagtcg agatgatcga acacgtaatg gccgcactag ccggtctgcg
     1801 tatcgacaac tgcactgttc agatcgatgc gggcgagtgc cccgggggcg atggatctag
     1861 tcaagctttc gtcgaagccc tggatcgtgc gggagccatc gagcaggacc ggatgcgcca
     1921 agccttggtg atggagcggg ggatcagcgt gcgtgaaggg gacgccgtcc ttgcggcgca
     1981 tcccggtgtg cctggaacgc tgacgctttc gtatcacctc gactatggcc agggttcgcc
     2041 gatcgcttcc cagagctttt gacttggcct gtcgccaaag tcctttcgcg acgagctagc
     2101 ggcgagccgc actttcgtgt tggagtcgga agccgaagcc cttcgcgccc aaggcatcgg
     2161 cacccgtgcg accgccgccg atatcttgac cttcggtcgg gacggggtaa taggcaatgt
     2221 cttgcgttac cctgacgaat gcgcgcgcca caaaacgctg gacattgtgg gcgacctcgc
     2281 tctcctgggt ttcgatcttc acggattcgt cgtggcgcac cggtctggtc accagaccaa
     2341 ccacgtcttg gcgcgacgcc tgctgcgaca tgccgcccaa tcgacggcgc cccgtgggct
     2401 cgaaagtacc tcccacgacc acgggtcgct cgacatacac gcgatcatga atttgctgcc
     2461 gcatcgctac ccttttttgc tagtcgacag ggtgctcgag ctcgagcctg ggcaccgggc
     2521 cctggcgatc aaaaacgtca gcgcgaacga gccgtatttc cgcgggcact ggccgggaca
     2581 ggcgatcatg cctggcgtgc ttatcgtcga ggcgctggcg caagcagccg gcatcttggt
     2641 tgccaagagc gtcgaacgac ggccgggctg ggtcgcgctc atcgcttcga tcaacggtgt
     2701 caagcttcgc cggccggtcg tgcctggcga tcagctccgg ttagaagtgt tcggccagaa
     2761 gatcaagtct aattcggcct gcgtctcggg ccaggccaag gtgggtgacg ccttggccgc
     2821 ggaggcaaag ctgcggtttg taatggtgga cgccgagcgt gccgcccgat cattcgacag
     2881 caacgctctc cttgaaaaag ttccaacccg ggttggataa gatcgatcgc gctacgggat
     2941 cgatcttttt ctccgtctat tttcggtcga gaggatggtt gccccatggc cactctgatc
     3001 gctgacacgg catgtatcga tccccgcgcg gacgttggtg atgatgtgga aatcgggccc
     3061 tactgcgtcg tcggcccaga tgtgacgatc ggccgtggta ctcgcctgat cgcccacgcc
     3121 tgcatccagg gcacgaccat tctcggcgaa gaaaacgtcg tgcatccgtt tgccgtgatc
     3181 ggtggggagc cccaagacgt gtcatttcgg ggaagtcaga cctgcctcga gatcggtgac
     3241 cgtaacgtga ttcgcgaagg ggtgacgatt caccgcggca gtgaaaaaga acaaggggtc
     3301 acgcggatcg gctcaaataa ccttctcatg gtcaacgtgc acgtcgcgca cgattgcgtt
     3361 atcgacgacc gagtcatcat tgccaacaac acgattctcg gtggtcacgg ccatgttgag
     3421 tcgtatgtga cgatctcggg cggagtgggc attcacccgt ttgtgtccat cggctcgtac
     3481 agctacgtag gcgctttgtc gcggatctat cacgacgtcc cgcggttcat gatcatcgac
     3541 ggcaacccat ccaaggttcg atgtatcaac gtggtcgggc tgaggcggca tgggatcagc
     3601 ccggaggcga tcgccgcctt gcatgaagca caccggctga tttatcgagc gcggatggcg
     3661 gtaagccacg cctccgagat ccttgagtcg cacggccaca tgtgccccga ggtcaaaagt
     3721 ttgctcgagt ttatcgaaga acagcatctg ggcaaacatg gccgggcgcg ggaacgacgg
     3781 agatcggcat gaaacgattg cgagtgggcg tcgtcggggt cggccatctg gggcagcacc
     3841 atgcacggat cttctcatct cttgccggcg tcgagttggt agcgatcgcg gatgcacggc
     3901 cagagcaggc gcatgccgtg gccgcacgat gcgggaccgt ggccctctgc gactacaggc
     3961 cacttctgga tattgtcgat gccgtctcca ttgccgtgcc gaccgcgcta caccgcgagg
     4021 tcgcgggggc cttcctggaa aaaggaatcc ccaccctcgt cgagaagccg atggctggca
     4081 cgcttttgga atcggagcag ctcgtggcgc tggcacgcgc tagggacacg ctcttgcagg
     4141 tcggccatat cgagcgattc aaccccgccc tgcgggctct cgacaagttg ccggtgcgtc
     4201 ccaagtttgt gaacgccgaa cggctctcga cttatacctt tcgctcgact gatatcggcg
     4261 tcgttctcga cctgatgatt catgacatcg acctggtgct ctcactgatc cccgcgccgg
     4321 tacggtcgct cgcagcggtc ggcgtaagcc ttttcggcga acatgaagac gtcgccaacg
     4381 cccggatcga atttgaggat gggagtgttg ccaacctcac tgctagccgg gcgagctatt
     4441 ccccctcgcg caaaatgcgg atctggggcg cggaggggta tgcttcgctc gattttgccg
     4501 ccaagcaggc caccctgatt cgcccttctg aacaactgaa gggcggccgg cttgacttgg
     4561 aaggcgtcga cctgagccag gcggcggcgg ttcgggagca tctcttcggc aaggtcttac
     4621 gcgtcgaccg cgtccaggag gaaggccggg aacccctgtc cctcgaacta gaagacttcg
     4681 tcagtgcagc gcgtgggctg tcgcgtccca gggtcagtgg agaagacgcc ctccgcgcgg
     4741 tcaggctggc cgaccagatt ttgcgttcac tggacgcgca tcgctgggat gccgaaaccg
     4801 gccccgcagc cgcgcagacg gtcgagtcgg tctcgatctt gcaaggccct cacgcgtggc
     4861 gaacccggag catgcggcaa gtttccaacc gtgcctaacc gcgaaggacc gtctgagttt
     4921 tcgcacggtg acaggctcgt cgcgcacctc gtcggcttcg tttcgcaaaa tgaa
//
LOCUS       DATAQL010001396         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3044395, whole genome shotgun sequence.
ACCESSION   DATAQL010001396 DATAQL010000000
VERSION     DATAQL010001396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3044395"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..378
                     /locus_tag="VHS97_23975"
     CDS             1..378
                     /locus_tag="VHS97_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451333.1"
                     /translation="MLLYAWASPTTLVGLLAGLLTLCSGGRVERRRGVLEFHGGFSQR
                     FADWIGFGAMTLGHVIIGRDPFCLAICRDHEHAHVRQVERWGAAFIPAYLLASVLAWW
                     LGGHYYLDNYFERDARRACGEPD"
     gene            432..1136
                     /locus_tag="VHS97_23980"
     CDS             432..1136
                     /locus_tag="VHS97_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX3451334.1"
                     /translation="MHQVAHIERHFTASELVRDIVIGMADGLTVPFALAAGLSGTNVA
                     TSLVVTAGLAEVAAGSIAMGLGGYLAAKSDAEHYVSEKHREETEILTLPHVEREEVAE
                     VFREYGLEAPEIEPILQAFANNHPAWVDFMMRFELGLEEPDPKRALRSAMTIAGAYIA
                     GGLIPLSPYMVVSETLKGLGISIFVTLVALLVFGFIKGHFTGTRPIRSALQTTLIGSI
                     AAAAAFLIARVFSGTG"
     gene            complement(1151..1882)
                     /locus_tag="VHS97_23985"
     CDS             complement(1151..1882)
                     /locus_tag="VHS97_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX3451335.1"
                     /translation="MIARMLLSACRPSSVVAGLMLVVLTGCHGLGKKHEANSIPQYGT
                     IDPHQPGELREVSQQPYVIEPPDELEITVFPAFPDWTPATFTVQADGNVDLGIAGDAY
                     VRGLTLAEAEERIAVQLNDQARQQKGNPSQPYRVSVRLSAGQSKYYYVLGTVTTQGRF
                     KSNGNETVLDAILQAGLRSNSLPEKAYLVRPHPVGGHDQVYKIDWFGIKDRGDTLTNY
                     QVFPGDRIVVPGTKPPGLISSLLGN"
     gene            2262..2519
                     /locus_tag="VHS97_23990"
     CDS             2262..2519
                     /locus_tag="VHS97_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3451336.1"
                     /translation="MEEITKVVQHFILQEFLPGEDPNELTDRTPLITGGILDSISTLK
                     LVVFLEEHFGITVEAYEAGVEHLNTIGLIAALVATKKKSAA"
     gene            complement(2538..>2867)
                     /locus_tag="VHS97_23995"
     CDS             complement(2538..>2867)
                     /locus_tag="VHS97_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX3451337.1"
                     /translation="EPVDLRRFRDCLDGELSNRNADYLAHRVPGVGLPPPAVIVAPSG
                     SFESWMRRRGKLGGQNKVPRMDNTGTLTGDLVQFLRETGRDGIEVSPHYSDMLVEAGA
                     LMSAVGG"
BASE COUNT          572 a          891 c          818 g          586 t
ORIGIN      
        1 ctgcttttgt atgcctgggc ctcgccaaca accctggttg gcctgctcgc cggtctttta
       61 accctctgtt cgggaggtcg cgtagagcgc cgccgtggcg tgctcgagtt tcacggcggc
      121 ttctcgcaaa gattcgccga ctggatcggc tttggtgcca tgaccctggg acacgtgatc
      181 atcggccgtg atccgttttg cctggcgatc tgccgagacc atgagcacgc ccacgtgcgg
      241 caggtcgagc ggtggggggc tgcgttcatc ccggcgtacc tgctcgcgag tgtcttggca
      301 tggtggctgg gcggacatta ctatctcgac aactacttcg agcgcgacgc gcgtcgggcc
      361 tgcggcgaac ccgattgatc ggggcgattg atcaagcctg cgcctccacc agccccagaa
      421 aagatagccc gatgcaccag gttgcccata tcgaacgtca tttcaccgcc agcgaacttg
      481 tgcgtgatat cgtgatcggg atggccgacg gactgaccgt gccgttcgca ctcgcggcgg
      541 gattgtcagg aacgaacgtc gcgacttcgc tggttgtcac ggccggcctg gccgaagtcg
      601 ccgcaggctc gatcgccatg ggactaggcg gctacctggc agccaagagc gacgccgaac
      661 attacgtcag cgagaaacac cgcgaagaga ccgagatcct gacccttccc cacgtcgagc
      721 gcgaggaagt cgccgaggtt tttcgtgaat acgggctgga agcgcctgaa atcgagccga
      781 tcctgcaggc atttgccaat aaccaccccg cctgggtcga tttcatgatg cggtttgagc
      841 tgggtcttga ggaacctgat cccaaacgag cattgcgcag cgccatgacg atcgccgggg
      901 cctatatcgc cggcggcctg ataccgctgt cgccttacat ggtcgtctct gagacgctca
      961 aaggactcgg gatctcaatc ttcgtcaccc tggtagcgtt gctcgtgttt ggcttcatca
     1021 agggacattt caccggcacg cgcccgattc ggagcgcact gcagactaca ttgatcggca
     1081 gtattgccgc ggccgctgca tttctgatcg cgcgggtgtt ttccggaacg ggttgatcag
     1141 ggcggccagc tcagttacct aaaaggctac tgataagtcc aggcggtttg gtccccggaa
     1201 ccacgatccg gtcgcccgga aacacctggt agttggtcaa cgtatcgccc cgatccttga
     1261 tgccgaacca atcgatcttg tagacctggt cgtggcctcc tacgggatgc ggacgaacca
     1321 gataggcctt ttccggcaaa ctgttggacc gcagtcctgc ctggagaatc gcatccagga
     1381 ccgtctcgtt accgttggac ttaaagcggc cctgggtcgt caccgttccg agcacgtaat
     1441 agtacttgct ctgaccggcg gacagcctga cagacacccg gtaaggttgg cttggattcc
     1501 ccttctgttg ccgggcctgg tcgttcaact ggacggcaat ccgctcttcg gcttccgcca
     1561 acgtcaggcc tcttacatat gcgtctcccg caatgccaag gtcgacgttg ccgtccgcct
     1621 ggactgtgaa agtcgcggga gtccaatcgg ggaacgccgg gaataccgtg atctccagct
     1681 catccggcgg ttcgatcacg tacggttgct gtgagacctc gcgcaactcg cccggttggt
     1741 gaggatcgat cgtgccgtac tgggggatgc tgttcgcttc gtgcttctta cccagcccat
     1801 gacagccagt cagtacgaca agcatgagac cggccacgac tgaggacggg cggcaagcgg
     1861 acaggagcat ccgtgcaatc atcgacgtac caccctcgct tagtccaggc ccgcggactt
     1921 atgctcgtcc ccacaaccgc tcttgatcgc gacgtgccca gaaacagtat tcgttcgtgc
     1981 gcaagcggtg taattcatca acgcccaact gtcaacgtaa tgatccttgt tgctcaattg
     2041 tcgacaaacg caccagactt gcggcgaaaa aaccgtatca attccctatt cggcaatcct
     2101 tcacgtaact ctctaggaaa actagagatt gaccggattg gccctggaga gtaacgtgcg
     2161 gcggtaaagg gttcggcctc atcgatcgca cgattgatca gccggcgaat gttcggtttc
     2221 tgcgcacttc gtcctggatg agggagtcgc ccgaggccat catggaagaa atcacaaagg
     2281 tcgttcagca cttcattctg caagagtttc tccccggcga ggatccaaac gagctcacgg
     2341 atcgaacgcc gctgatcacc gggggaatcc tcgattccat cagcacgctg aagctcgtcg
     2401 ttttcctcga ggaacacttt ggtatcacgg tcgaggcgta cgaggcgggc gtcgaacacc
     2461 tgaacacaat cgggctgatc gccgcacttg tcgccaccaa gaagaagtcg gctgcctgaa
     2521 atgcaacgtc ccccgaatta ccccccgacg gcgctcatca gagcgcctgc ctcgaccaac
     2581 atatccgagt agtggggcga cacctcgatg ccgtcccgac ccgtctcgcg cagaaactga
     2641 accagatcac ccgtgagcgt gcccgtattg tccatcctgg gaaccttgtt ttgcccgccc
     2701 agcttcccgc gccggcgcat ccaggactca aaactccccg acggtgccac aatcaccgca
     2761 ggcggcggca gccccacacc cggaacccgg tgagccagat aatcggcatt gcgatttgag
     2821 agctcaccgt ccaggcaatc gcgaaaccgc cgcaaatcga ccggttc
//
LOCUS       DATAQL010001397         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4110962, whole genome shotgun sequence.
ACCESSION   DATAQL010001397 DATAQL010000000
VERSION     DATAQL010001397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4110962"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VHS97_24000"
     CDS             <1..187
                     /locus_tag="VHS97_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451338.1"
                     /translation="LVECELAWLSPDKLKAIRLLGMQPIDAIDDLDVARIFLASYVIR
                     GSAGDPAHQERRPQWIR"
     gene            667..1494
                     /locus_tag="VHS97_24005"
     CDS             667..1494
                     /locus_tag="VHS97_24005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03000 domain-containing protein"
                     /protein_id="HEX3451339.1"
                     /translation="MKRFWSNFAGASVGALALALSMPATASAQWGSSGGSNGGSSHGS
                     LFSGHGSSGGSSHGSLFSGHGSSGGSKHSFFSGHGSSGGSSGGSKHSLFSGHGSSGGS
                     SHGSLFSGHGSSGGSSHGSLFSGHGSSGGSNGGSYGSNGGSYGSGGGYFMESNDYVPS
                     PTSYVVPTSYVAAPVVQPSTGVAYLNVNVPADAKVYLQDKLMTIGGTDRRFVTPEIQA
                     GVQYLYTVKVEIVRNGQTVSKTAQATVVAGQEVAVAVAFDAQNQQELVASAALVAGR"
     gene            1639..2220
                     /locus_tag="VHS97_24010"
     CDS             1639..2220
                     /locus_tag="VHS97_24010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451340.1"
                     /translation="MSPCADERPEALECALAPKVPRSRLLRWAINLALAFHVVAIIIA
                     PAAVSPSSDLVQAGWNLFQPYLQILYLNHGYHFFAPEPGDSTLLAFQAERPDGTVVSG
                     RIPNRDIEPRLLYHRHFMLTEQMKDAPAELRDEWLGSYAEHICRKNGAARVRLTGQTH
                     HLPTMEMVRSGVRLNDPSSYEDEDLGVFECDAH"
     gene            2205..>3137
                     /locus_tag="VHS97_24015"
     CDS             2205..>3137
                     /locus_tag="VHS97_24015"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX3451341.1"
                     /translation="MRRALNPVCRYSLNVLRTVEREWNAFWYTPADPTLLGLLRVLTG
                     LMLVYTHAVWGLALDDFFGKTSWLSPSLVRAIESQQYTYSFWSWIPHQWMWPAYALSM
                     AVFVCFTFGVWTRIASVMALFAAISYTSRVPAALFGLDQINVMLTLYLAIGPSGRSLS
                     LDQWLARRRLGPQAPPSAPSVTANVAQRLIQVHMCVIYLFAGISKLQGPSWWSGEAMW
                     RAFANLEYQSLDMTWLAWHPWLVNIMSHVSVAWEIAFCVLIWKPLWRPLMLAVAVVLH
                     VGIGACLGMWTFGLIMLVGCASFLPNEAVRELVDR"
BASE COUNT          524 a          905 c         1010 g          698 t
ORIGIN      
        1 cctggtggag tgcgagctcg cctggctctc gcccgacaaa ctcaaggcca tccgccttct
       61 gggcatgcag ccaatcgacg ccatcgacga tttggacgtc gcccggatct tcctcgcctc
      121 ttacgtaatc cgcggcagtg ctggtgaccc cgcgcaccaa gagaggcggc cgcaatggat
      181 tcggtgaatt aagatgcgtg aattcgtcgg ctggccagcg ccaaacgtcc tcgtgagcgg
      241 tcgggcaaag tcttgcgctt taaaaaaacg cgctgaaagt tccgctaatc gtttgacatt
      301 gcccaaaata cccagtattc tcagtcttag tagtattcgt agaggcctta cattgacgag
      361 cacacggggt tcgctctgca gctcggtctg cgggtaactc atttcgctcg ctcttgaacc
      421 gcacaatcac tctgaaagca gtgggcgagt ttcctggttg ttcatgcata ggcgcgtgga
      481 tatcctgacc ttatcgcgat cggcgcacgg cagggccggg cctgatccgc gaccgacgtg
      541 ggtgtgggtg tgcgacgaga gaggtctcga atggatcgat ctgtcagttc ggattgaggc
      601 ggcacggttc gcgtttcgtt tttcgatttc acgtaagagc gcttggcaac acttgaggat
      661 ttgacgatga agagattttg gtcgaatttt gccggggcat cggtgggcgc actggccttg
      721 gccttgagca tgccggcaac ggcttcggct caatggggtt cgagcggcgg ctccaacggc
      781 ggcagctcgc acgggtcgct gttctcgggt cacggctcca gcggcggcag ctcgcacggg
      841 tcgctgttct cgggccacgg ctccagcggt ggcagtaagc actcgttctt ctcgggccac
      901 ggctccagcg gtggttcgag cggcggcagc aagcactcgt tgttctcggg tcatggctca
      961 agcggcggca gctcgcacgg gtcgttgttc tcgggccacg gttctagcgg cggcagctca
     1021 cacgggtcac tgttctcggg ccacggttcg agcggcggct ccaacggcgg ttcgtacggc
     1081 tccaacggcg gctcgtacgg ctccggcggc ggctacttta tggaatctaa cgactacgtc
     1141 ccgtcgccca cttcctatgt ggtcccgacg agctacgtgg cggcgcccgt cgttcagccg
     1201 agcaccggcg tggcgtatct caatgtcaac gttcccgcag atgccaaggt ttatctccag
     1261 gacaagctga tgacgatcgg tggcaccgac cgccgatttg ttacaccgga aatacaggcc
     1321 ggcgttcagt acctttacac cgtgaaggtt gagattgtgc ggaacggcca gacggtttcg
     1381 aagacggcgc aggccacagt cgtggccggc caagaggttg cggtcgcggt tgcctttgat
     1441 gctcagaacc agcaggagct ggtggcctca gcagctctgg tcgcagggcg ctaatcgctg
     1501 ggccgtgacc atcgctcgtt gttccacgtg cagattttgt gggttgcgct atcggggaag
     1561 gcggatggat ccgtcttccc cgcggctatt ggcggtgtgg gaaacatgat cgcgggcaca
     1621 gaaaacggag atcgcggaat gtccccttgc gcggatgaac gaccggaggc gctcgagtgc
     1681 gccctggctc cgaaagttcc tcggtccagg ttgctgcgct gggcaatcaa tctggcactg
     1741 gcgtttcacg tcgtggcgat catcattgca cccgcggcgg tgagcccttc ttcggatctg
     1801 gttcaagcgg gctggaacct gtttcagccg tatcttcaga tcctttacct gaaccacggt
     1861 taccattttt tcgcgcccga gccgggcgac agcacgctgc tggcatttca ggctgagcgg
     1921 cccgacggga ccgtggtgag cgggcggatt cccaaccgcg acatcgagcc tcgcttgttg
     1981 tatcaccgtc atttcatgct gaccgagcag atgaaagacg ctccagcaga gctgcgggac
     2041 gaatggctcg gatcgtatgc cgagcacatc tgtcgcaaaa atggtgcggc cagggtcagg
     2101 ctgaccgggc agacccatca tctccccacc atggagatgg ttcgttcggg cgtgcggtta
     2161 aacgatccgt cgagctacga ggatgaagat ctgggggtct tcgaatgcga cgcgcactga
     2221 atcctgtttg ccgatactcg ttaaacgtct tacggacggt cgagcgggaa tggaatgcgt
     2281 tctggtacac tcccgccgac ccgactttgc tgggcctctt acgcgtcttg actggcctga
     2341 tgttggtcta cacccacgcg gtctggggcc tggcgctcga tgacttcttc ggcaagacga
     2401 gctggctgtc gccgagcttg gttcgtgcaa tcgagtctca gcagtacacg tattccttct
     2461 ggtcttggat accccaccag tggatgtggc cggcgtatgc gctgtcgatg gccgtgtttg
     2521 tgtgtttcac gttcggagtc tggacgcgaa tcgcatccgt gatggcgctc tttgccgcga
     2581 tttcctacac cagccgcgtc ccggcggctt tgttcgggct cgatcagatc aacgtgatgc
     2641 tgacccttta cctggcgatt gggcccagcg gccggagctt gtcgctggac caatggctgg
     2701 cccggcgccg gctgggcccg caggcgcctc cgtcggcgcc gagcgtgacg gccaacgtag
     2761 cccagcggct aattcaagta cacatgtgcg tgatctatct cttcgcgggc atctccaagc
     2821 tgcaagggcc gtcgtggtgg agtggcgagg cgatgtggcg ggcgtttgcc aacctggagt
     2881 atcagtcgct cgacatgacc tggctggcgt ggcacccctg gctggtcaac atcatgtcgc
     2941 acgtctcggt ggcctgggag atcgcgtttt gcgtgttgat ctggaagccc ctgtggcggc
     3001 cgctcatgct cgccgtcgcg gtggtgctcc acgtcggaat cggcgcttgc ctggggatgt
     3061 ggaccttcgg gttgatcatg ctggtcggct gtgcgtcgtt cttgcccaat gaggctgtgc
     3121 gagagctggt agaccgg
//
LOCUS       DATAQL010001398         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3499839, whole genome shotgun sequence.
ACCESSION   DATAQL010001398 DATAQL010000000
VERSION     DATAQL010001398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499839"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VHS97_24020"
     CDS             <1..697
                     /locus_tag="VHS97_24020"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX3451342.1"
                     /translation="AVYIDWTNDIIDPDDPVPVQIAPPARFAEAMATRGIGDQTLVVA
                     VDHAGGQFATRLWWALKYYGHDNARVLDRGWNAWKTEERPVEAGETTPPRAHFTPRIK
                     AGLRVTALQLAGRLDHPDLDWQLLDARDPGQYSAARRRGPRGGHIPGAINVPRELFFA
                     PQGGFLPLDDLRGRVDELQIRSDRPTVAYCNGGVAATVVLFNLARLGYADLANYDGSW
                     NEWSSRLDLPVEP"
     gene            981..1616
                     /locus_tag="VHS97_24025"
     CDS             981..1616
                     /locus_tag="VHS97_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3451343.1"
                     /translation="MREHDPKKPGGPNGHSRRDFLRGSGAAAATAVLTGQATLALDEA
                     QAAQAAEPKILSGTVELTLRVNGQDRSCAVEPRSTLLDTLRHRLDVTGPKRVCDRASC
                     GACTAIVDGDPVYSCTTLAVSCQGKSIQTLESFDTGSQGVPYAFHQNDALMCGYCTPG
                     FVTSCKALLDKNPNPSLEEVKKGLEGNICRCGTYVGVMQAALAAAKAMKGA"
     gene            1622..3784
                     /locus_tag="VHS97_24030"
     CDS             1622..3784
                     /locus_tag="VHS97_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3451344.1"
                     /translation="MATWPQNPRLIGTRITRLDGMAKASGQAKYPSDVRPQGTLFGVM
                     LYSPYAHATVKSIDISAAEQMPGVKAVIAIAAAGKPLRYHGDDIAAVAAETEEQARDA
                     VRAIKVEYEVLPHIATEALSMAQGAPPVFKPNNVRPGRGQTKGKPEEAMAAAEVTSEG
                     TYSVPLITHVCLESHGLTVMLEEGGDKVKAWASTQNVGTTGVAKDLADAVGVPVTNVT
                     VLTDYMGGGFGSKFGADIWGKTAAQLSKKAGGRPVKMFLDRVQEHLAAGNRPSGWAHI
                     KMGANRDGKVVSMIAEARGTGGVNPGADVILPYVYSFPNTSVAQSTVMTNFGGSRAMR
                     APRHPQSCLLTEAAMDDLADKLGMDPIEFRLKNLDPNDFHTPIYEAELKMGSELIGWR
                     DKRKPRGQTGTGPIRHGMGLGLHQWGGGGTRDKKVSCTINPDGSVELRAATQDIGTGA
                     RTILAIIAAEVLGLQPTDIFSNIGNSTFPPGQPSGGSTTTPSMSPPCLDAATKARDAL
                     FNKIAPAVQATPADLSLKDSQLWVSGKPVMSWKDACRKLGTSTISEMGSYDPNEKGLS
                     STGVGGCQFAEVAVDIETGMVRVTKIVAIQDTGLIIDKMTWESQVYGGVIGGLNYGLF
                     EERIVDPTTGIMVNPDMEWYKLAGAADIPEIIVRAYEPPEQKARGVIGVGEPPTISTA
                     AAIGNAVTNAIGVRVSEWPMTPRNVLNALATASKEGKA"
     gene            3790..4782
                     /locus_tag="VHS97_24035"
     CDS             3790..4782
                     /locus_tag="VHS97_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX3451345.1"
                     /translation="MKAFEYAAPGTIEDAVKLLGSPTAAALSGGTDLIGRMKDYVTSP
                     ERVVYLKDIKALSGVEGDGKTAGMTIGAGTKLSDIVVSTAIKEVYPALWQATVEVGSP
                     QIRNMATVGGNLLQRPRCWYFRAGHGILGMKDGKSLVRAGDNRYHAIFLTDGDALFVT
                     PSSLAVALSALDAQATITGPKGERTVKVEALYQVPKTENDSELLIGPGEILTNVAIPP
                     ATGKNASYEARQKQAQDWPLVLASVNLAMEGDSVSGAKVVVYGVAPIPWRSTAAEKAI
                     AGKRVTIETAAAAGEAAAEGAVPLSMNGYKVPLTKTVVKRALLAAVGNRYWEEA"
BASE COUNT         1000 a         1495 c         1517 g          840 t
ORIGIN      
        1 cgcggtctac attgactgga ccaacgacat catcgacccc gacgatcccg tccccgtcca
       61 gatcgcgccc cccgcgcggt ttgccgaggc gatggcgaca cggggcatcg gtgatcaaac
      121 tctggtggtt gccgtcgatc atgcgggcgg ccagtttgcg acccgactgt ggtgggcgct
      181 caaatattac ggccatgaca acgcgcgcgt gctcgaccgc gggtggaacg cgtggaagac
      241 ggaagagcgg ccagtcgagg ctggggaaac gacgccaccg cgcgcccact tcacgccgcg
      301 tatcaaggcc ggattgcgtg tcacggccct tcaacttgcc ggccgcctgg atcatcccga
      361 tctcgattgg caacttcttg acgcgcgaga cccgggtcaa tattccgctg cccggcgacg
      421 cggtccgcgc ggcggccaca tccccggcgc gatcaacgtt ccacgcgagt tgttcttcgc
      481 gccccaggga ggatttctac cgctggacga tttgcgagga cgagtcgacg aacttcagat
      541 ccgatccgac cggcccacgg tcgcctactg taacggtggc gtcgcggcga ccgtcgtcct
      601 tttcaacctg gctcgactgg gctacgccga tctggccaat tacgacgggt cgtggaacga
      661 atggtcgagc cggctcgatc tgcccgtcga gccctgacat ttccaatccg gcagtctcga
      721 tgccgctttt ttttcgacgc cggcaatgcc atgatacgag caattggcga cagaaaaata
      781 gcggcttatc gattgatatt gagataggcc tctcttgccg ccgacaaaca ggcgaagtag
      841 tttggtggtt tcgaacgaga acgtatacgg gacagcggta tttgacacgg ctcgaccctt
      901 ttaagaggta aaagccgaac cggtgcccga gatcaatgga gtggactttg tgggcaaagt
      961 gaaaagggga gattcgctcg atgcgagaac atgacccgaa gaaacccggc gggcccaatg
     1021 gtcacagccg gcgcgacttc ctgcgggggt ctggtgccgc ggccgccacg gcagtattga
     1081 cggggcaggc caccttggcg ctcgacgagg cgcaggccgc gcaagcagcc gagcccaaga
     1141 tcctctcggg cacggttgag ctcacgctca gagttaatgg ccaggatcgc tcatgcgcgg
     1201 tcgagccacg cagtactctg ctcgacaccc tgcgccaccg gctggatgtg acgggcccca
     1261 agcgggtctg cgatcgcgcg agttgtggag cgtgcacggc aatcgtcgac ggcgatccgg
     1321 tctattcctg caccacgctg gccgtctcgt gccagggcaa gtcaatccag acgctcgaga
     1381 gtttcgatac aggctcccaa ggcgtgcctt acgccttcca tcaaaatgac gccctcatgt
     1441 gcggctattg cactccgggg tttgtcacct cttgtaaagc cctcctcgac aaaaacccca
     1501 atccgagcct cgaagaggtc aagaaggggc tcgagggcaa catctgtcgc tgcggcacgt
     1561 acgtcggcgt catgcaagcg gcgctcgccg cggccaaagc catgaaagga gcgtgaatac
     1621 catggcaact tggccccaaa atccacgttt gatcggcacc cggatcaccc ggctcgacgg
     1681 aatggcaaaa gcgtcgggac aagccaaata tccctccgac gttcggccgc agggcacgct
     1741 cttcggcgtg atgctctaca gtccttacgc ccatgccacg gtcaagtcga tcgatatctc
     1801 tgccgcagag cagatgcctg gcgtcaaggc cgtcatcgcg atcgcggcgg cgggaaagcc
     1861 cctcaggtat cacggcgacg atatcgccgc tgtagccgct gagaccgaag aacaggcccg
     1921 cgacgccgtg cgcgccatca aggtcgaata cgaagtgctg cctcacatcg ccaccgaggc
     1981 cttgtcgatg gctcaagggg cgcccccagt tttcaagccg aacaatgtca ggccagggcg
     2041 gggtcagacc aagggaaagc ccgaggaagc gatggccgcg gccgaggtaa cgagcgaggg
     2101 aacttactcg gtgcccttga tcacgcacgt ctgcctcgag tcgcacggct tgaccgtgat
     2161 gcttgaagag ggtggagaca aggtcaaggc gtgggcaagc acgcaaaacg tcggaactac
     2221 tggcgttgct aaagacctcg cggacgcagt gggcgttccg gtgacgaacg tcacggttct
     2281 gacggattat atggggggcg gattcggctc gaaatttggc gccgatattt ggggcaagac
     2341 ggcagcacag ctttcgaaaa aggctggcgg ccggccggtg aagatgttcc tcgatcgtgt
     2401 ccaggaacac ctcgccgcag gcaaccgtcc cagcggctgg gctcacatca agatgggggc
     2461 taaccgcgac ggtaaggtcg tctccatgat cgccgaggca cgcggcaccg gcggggtcaa
     2521 ccccggggcc gacgtaatcc tgccttacgt ctatagtttt cccaatacgt ccgtcgcgca
     2581 atcaaccgtc atgaccaact ttggcggcag ccgcgccatg cgggctccga ggcatcctca
     2641 gagctgcttg ctcacggaag cggccatgga tgaccttgcc gacaagctgg gaatggatcc
     2701 gatcgaattc cggctcaaga accttgaccc caatgatttc cataccccca tctacgaggc
     2761 ggagctcaag atggggtcgg aattgatcgg ctggcgcgac aagcgcaagc ctcgcggtca
     2821 gaccggcacc ggaccgatcc ggcacgggat gggcctcggg ctgcatcagt ggggcggcgg
     2881 tggcacccgc gacaagaaag tctcctgcac cattaacccc gatgggtcgg tcgaactccg
     2941 agccgctacc caagacatcg gcacgggtgc gcgcacgata ctggcgatca tcgccgcgga
     3001 agtcctcggg ctccaaccga ccgacatctt ctcgaatatc ggcaactcga ccttcccgcc
     3061 ggggcagccc tcgggaggtt cgaccacgac gccctcgatg tcgccgccat gcctggacgc
     3121 ggccaccaag gcacgtgacg ccttgttcaa caagatcgcg ccggccgtgc aggcgacgcc
     3181 ggcggaccta tcgctcaaag atagtcaact ctgggtctcg ggcaagccgg tgatgtcgtg
     3241 gaaggatgcg tgccgcaagc tcgggacgtc gacgatttcg gagatgggtt cctacgatcc
     3301 caacgagaag gggctatcca gcaccggggt cggcggctgc cagtttgccg aagtggctgt
     3361 tgacatcgag accggaatgg tgagggtcac gaaaatcgtc gccattcaag acaccggcct
     3421 catcatcgac aagatgacct gggaaagcca ggtttacggt ggtgtgatcg gcggtttgaa
     3481 ctacggactc ttcgaggagc ggatcgtcga cccgaccaca ggcatcatgg ttaatcccga
     3541 catggaatgg tacaagctgg ccggtgcggc cgacattccc gagatcatcg tgcgggccta
     3601 cgagccgccc gagcaaaagg cacgtggcgt gatcggtgtg ggcgagcccc cgacgatctc
     3661 gacagcggcc gccatcggca acgcggtcac taacgcaatc ggcgtacgag tttctgagtg
     3721 gccgatgact ccccgaaacg tcttgaacgc cctggccacg gcctctaaag aagggaaggc
     3781 gtaaaaacta tgaaagcttt tgagtacgct gcacccggca cgatcgaaga cgccgtcaag
     3841 cttttgggct cgccgaccgc ggcggccctt tcgggcggca cagacttgat cggccggatg
     3901 aaagactacg tgacttcgcc cgaacgtgtg gtctatttga aggacatcaa ggcgctttcc
     3961 ggcgttgagg gcgacggcaa gaccgccgga atgacgatcg gcgcggggac aaagctctcg
     4021 gatatcgtcg tgagcacggc tatcaaagag gtctatcccg cgctctggca agcgaccgtc
     4081 gaggtcggct cgccacagat tcgcaacatg gccaccgtcg gcggcaactt actgcaacga
     4141 ccgcgctgct ggtacttccg cgccggccac ggaatcctgg gcatgaaaga cggcaagagt
     4201 ttggtccgcg cgggcgataa tcgctatcac gcgatcttcc tgaccgacgg cgatgctctt
     4261 ttcgtcactc cctccagtct ggccgtggcc ctgagcgcct tggacgctca ggcgacgatc
     4321 accgggccca agggggaacg aaccgtaaag gtcgaagctc tctatcaggt ccccaagacc
     4381 gagaatgata gcgagctctt gatcgggcct ggcgagatcc tcaccaacgt tgccatcccg
     4441 ccggccactg ggaaaaatgc ctcctacgaa gccagacaaa agcaggcgca agattggccg
     4501 ctcgtcttgg cttcggtcaa cctggcgatg gaaggcgaca gcgtctccgg cgccaaggtc
     4561 gtggtttatg gcgtagcgcc gataccgtgg cggagcacgg cggcggagaa ggcgatcgcc
     4621 ggcaagcgtg tgacgatcga gacggccgcc gccgccggcg aggccgcggc ggaaggcgcg
     4681 gttccactgt ccatgaacgg ctacaaggtc ccactgacca agactgtggt caagcgggcc
     4741 ctgctcgcgg ctgtcggcaa ccgttactgg gaggaggctt gaatagcctc aagtcgatgg
     4801 acaccgccaa accgtctgac cttccggtct tgagcacgaa gctctcgccc tg
//
LOCUS       DATAQL010001399         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3499843, whole genome shotgun sequence.
ACCESSION   DATAQL010001399 DATAQL010000000
VERSION     DATAQL010001399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499843"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VHS97_24040"
     CDS             <1..589
                     /locus_tag="VHS97_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3451346.1"
                     /translation="SITLDCRLDEAIPLLRQDPGKLKQILYNLLSNAIKFTPEGGRVT
                     LRARPEGRFVVVEVEDTGIGIADEDRETIFEKFRQAKVPGQGENVLTREHQGTGLGLS
                     IVRELTKLLGGDVHLESRTGHGSTFTVRIPMMLPGNRKFEVTLSDERIDLTKARRIEA
                     HPPLPHLPLGRPGPILPDGEVYDPARGRKRNPSAT"
     gene            618..1604
                     /locus_tag="VHS97_24045"
     CDS             618..1604
                     /locus_tag="VHS97_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3451347.1"
                     /translation="MARQGDGPPVVGIDLGGTKILAGVVSRDHRIMGRAKRTTPAKEG
                     GPAILATVISCVDEALTAAGVSRDVIAGAGIGSPGPLDVNAGVILFSANLNVRNFPIG
                     PDVARALDRPVIVRNDVRVGGYAEFRLGAGRGFRDIIAVFVGTGIGGCLIRGGEIIAG
                     ATGNAGELGHMIIKAGGPRCGCGNRGCLEALASKTAIARRVDKAVRKGLQTVLGEKMA
                     KKGGRLKSRDLAEAVASRDLVALKEVQRAGHFLGIGLGSLINLLGPEIVIVGGGVAGA
                     LGDPYLEIVRASARTQAITDPQGKIGIDRAALGDDAGILGAALLAREHFALN"
     gene            complement(1615..3699)
                     /locus_tag="VHS97_24050"
     CDS             complement(1615..3699)
                     /locus_tag="VHS97_24050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3451348.1"
                     /translation="MRVNPRRPQSAEEVRLRIARLECIPLRPLTARMLSTAVAPETDD
                     EFDAAPRSERRSIFQLDPGWVLAETRAAGRVESLALLAGTPWWQSTPGSTAAGDFIGR
                     LWRYSVAVSVAARSLAREAGDPDPDGVARAGLLSRLGCWAVTAVDPDWMARWWQDGSV
                     LSRRQRELADLGTDLDDLGRRLAERWGCEPLVVDAVWLHGTHGSALQALAAEPTRLAY
                     IQQACRWAKQTPWSFAAGSSAEGMQTDPRLRILVAEVQARTGLAFVATDANSYEERMT
                     QQNAQLRMLLASARRDRESADRFLKALASSDPALGPEDWALEAAKIWCAEPEVSAASV
                     VWVEPPTSLATVKQPNDASAPASRETPQSTSPDPRPPALVVPLKGQGRVRALVQLWSD
                     HGPSDLQKRLEQRIALGAWESWAMMVADRALLERRVQAVVASLRDRIETEEIQLGQRK
                     LDALGEFAGGAGHELNNPLAVIVGRAQLLLARTSDPETARSLHIMLNQAGRAHRILRD
                     LMFVARPLAPRPRNFRPSDLLRACLRDFQDECAARGIRLASEIDDSIPVVACLDPDAL
                     RHLADILMRNAMDATPSGGKISVRSSMHVDELSWWFGDSGKGIAPHEAAHLFDPFFCG
                     RQAGRGLGLGLPRAARIVEQARGRLSWTSHPGRGSAFQIDLPLAPQIASAGQIPRSMI
                     ASSISGDRPPKS"
     gene            complement(3814..>4455)
                     /locus_tag="VHS97_24055"
     CDS             complement(3814..>4455)
                     /locus_tag="VHS97_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3451349.1"
                     /translation="PLTGRGRTTSLISQDGTYTTMKTVFTTGEAAKVCKVSQQTIIRC
                     FDSGQLKGFRVPGSRFRRIPRDALYRFMKENGIPTDALESGRRRVLIVDDDQGVVDLI
                     SEVLAGDARFETKVVNNGFGAGILAKEYHPDLIILDVMLPDINGQQVCELLRKDPTMS
                     DIKIICISGMIEEDKIQELRESGADDFLHKPLDIDELMRRVCRLLDMESSLIS"
BASE COUNT          854 a         1356 c         1419 g          826 t
ORIGIN      
        1 gagcatcacc ctcgactgcc ggctcgacga ggcgatcccg cttttgcgtc aagatcccgg
       61 caaactcaag caaatcctgt ataacttgct ttcaaacgcg atcaagttca ctcccgaggg
      121 gggccgtgtg acgcttcggg cccggccgga aggccggttc gtcgtcgtcg aagtggaaga
      181 cactggaatc ggtatcgcgg acgaagaccg ggaaacgatc ttcgaaaaat tccgccaggc
      241 caaggtgcct ggccagggtg aaaacgtcct gacccgcgag caccaaggga ctgggttggg
      301 cctttccatc gttcgtgagc tgaccaagct cttgggcggc gatgtccatc tcgagagccg
      361 gaccggccac ggcagcacgt tcacggtgcg gatcccgatg atgctgccag gaaatcgcaa
      421 gtttgaggtt accctctctg acgagcggat cgacttgacc aaggcgaggc gtatcgaggc
      481 gcaccccccc ctgcctcacc ttcctcttgg gcggcccggc ccgattttac ccgatggcga
      541 agtctatgat ccggcaaggg gccgcaaacg gaatccgtcg gctacctagg cctcactgac
      601 gtttggagcc ttcgcgaatg gccaggcagg gtgatggacc cccggttgtc ggtatcgacc
      661 tgggtggcac caagatcttg gcgggtgtgg tcagccgcga ccatcggatc atgggtcgcg
      721 ccaagcggac gactccggcg aaggaggggg gaccggccat cctggccaca gtgatcagtt
      781 gcgtcgatga agcgctgacg gccgccggcg tgtcgcgcga cgtgatcgca ggcgcaggta
      841 tcggatctcc gggtccctta gacgtcaacg cgggcgttat cctcttcagc gccaatttga
      901 acgtcaggaa tttcccaatc ggaccggacg tcgccagggc gctggatcgc cctgtcatcg
      961 tgcgaaacga cgttcgcgtc ggcggatatg cggaattccg gctcggagcc ggtcgcggct
     1021 ttcgtgacat tatcgcggta ttcgtgggga ccgggatcgg cggttgcttg attcgtggag
     1081 gcgagattat tgcgggtgcc accggcaacg caggcgaact ggggcatatg atcatcaagg
     1141 ccggaggacc gcggtgtggt tgcggtaatc ggggttgcct ggaagccttg gcgagcaaga
     1201 ccgccatcgc gcggcgagtc gataaggctg tccgcaaagg gcttcagaca gtgctcggcg
     1261 aaaagatggc caagaagggg ggccggctca agagtcgcga cctcgccgag gcagtcgctt
     1321 ccagagactt ggtcgcgctc aaggaagttc agcgggcagg gcatttcctg ggaattggtt
     1381 tggggagcct gattaacctg ttgggtcccg agatcgtcat cgtcggcgga ggagtagccg
     1441 gtgcactcgg cgatccgtat ctcgagattg tcagagcgtc agcccggacc caggcgatca
     1501 ctgatccgca gggaaagatt ggcatcgatc gcgcagcctt gggcgacgac gccggcatcc
     1561 tgggggcggc cctgctggcc cgcgagcatt tcgctcttaa ctgaaccgcc gtgctcagga
     1621 tttgggcgga cgatcgccac taatggaaga agcgatcatg gagcgcggga tttggccggc
     1681 cgatgcgatt tgcggtgcga gaggtaggtc tatctggaaa gcagatcctc ggccgggatg
     1741 tgaagtccaa ctcaggcgac cgcgtgcctg ctcgacgatc cgagcggcgc gtggcaaacc
     1801 gaggcccaat ccccttccgg cttgccgtcc acagaaaaac ggatcgaaaa gatgggccgc
     1861 ttcgtggggt gcgatcccct tgccggagtc gccaaaccac cacgacagct cgtcgacatg
     1921 catactcgac ctgaccgaga tcttgccgcc ggaaggagtc gcgtccatgg cgttgcgcat
     1981 caggatgtcc gcgagatgcc gcagagcgtc cggatcgagg cacgcgacga cgggtattga
     2041 atcgtcgatt tcgcttgcga gccgaatgcc cctggcagcg cactcgtctt gaaaatcccg
     2101 caaacacgcg cggagaagat cggaaggtcg gaagttccgc ggtctgggtg cgagcggccg
     2161 cgccacgaac atcaggtcgc ggagaatgcg atgagcacgc ccagcctgat tgagcatgat
     2221 gtgcagtgac cgtgcggtct cgggatcgct agtccgagcc agcagcaatt gcgcgcggcc
     2281 gacaatgacc gcgagcggat tgttgagctc atggccggct ccccctgcga actcacctag
     2341 cgcgtcgagc tttcgctggc cgagctggat ttcctcggtt tcgattcgat cgcgcaacga
     2401 tgcgacgacg gcttggaccc ggcgctcgag caaggcgcga tcggcaacca tcatcgccca
     2461 tgattcccag gcgcctaggg ctatccgttg ctcgagccgc ttttgcaggt cggatggtcc
     2521 gtgatcgctc cacaattgaa cgagcgcgcg aacgcgcccc tgccctttca acgggaccac
     2581 cagcgcggga ggacgcggat ccggtgaggt tgactgcgga gtttcgcggc ttgccggagc
     2641 ggacgcgtcg ttcggctgtt ttacggtggc aagagacgta ggcggttcca cccagaccac
     2701 actcgccgcg ctgacttccg gctcggcgca ccagatcttg gcggcctcta aggcccagtc
     2761 ttccggcccg agtgcggggt cggaactcgc gagtgctttg agaaaccgat cggcactttc
     2821 ccggtcccgc cgcgcggacg ccaacagcat ccgcaattgt gcattctgct gcgtcattcg
     2881 ctcttcataa gaattcgcat cggtggcgac gaaagctagt ccggttcggg cctgaacttc
     2941 agcgaccaaa atccgtagcc gagggtccgt ttgcatcccc tccgccgacg agccagccgc
     3001 aaatgaccac ggcgtctgct tggcccaacg gcatgcttgc tggatgtagg caaggcgggt
     3061 gggttcggcc gccagtgcct ggagagccga accgtgagtg ccatgcaacc agacagcatc
     3121 gacgaccagc ggctcgcagc cccagcgctc ggccaagcgg cgaccgaggt cgtccaggtc
     3181 ggtgcccagg tcggcaagct ctcgttgccg ccggctgagg acactgccgt cttgccacca
     3241 cctggccatc caatcgggat cgacggccgt tacggcccag caaccaagac ggctaaggag
     3301 accagcgcgt gcgacgccgt ccggatcggg atcgccagcc tcgcgcgcga gcgaccgcgc
     3361 agccacgctg accgctaccg agtaacgcca gagccgaccg atgaagtcgc cagcagccgt
     3421 ggaaccagga gtggactgcc accacggcgt tccggccagg agggcgagcg attcgacgcg
     3481 accggcggcg cgagtttctg cgagcaccca accgggatcg agctggaata ttgaacgccg
     3541 ctctgagcgc ggagccgcgt caaactcgtc gtcggtctcc ggggcaaccg ccgtgctcag
     3601 catccgtgcc gtcagcgggc gcagcggaat gcactcgagc cgcgcaatac ggagtcgaac
     3661 ttcctctgcg gattgcggtc gacgggggtt gactcgcacc gggcgggctc cttcccgact
     3721 tccgaagcgg aatgtccttt tctcaatcta cgctcttcgc atccatgcac tcacggcagc
     3781 ctggcatggg gccaggtcgc atgaaggatt ccgtcagcta attaggctgg actccatgtc
     3841 cagcagccga caaacgcgcc gcattaattc atcgatatcg agcggcttgt gcaggaaatc
     3901 gtcggctcct gattcgcgta gttcctgaat cttgtcttcc tcaatcatgc ccgagatgca
     3961 gatgatcttg atgtcggaca tggtcgggtc tttgcgaagc agttcgcaaa cttgttgacc
     4021 attgatgtcg ggaagcatca cgtccaagat gatcaggtcc gggtggtatt ccttggcgag
     4081 gattccggca ccgaaaccat tgttgacgac tttggtctca aaccgggcat cgccagccaa
     4141 gacctccgag atcaaatcga caacgccctg atcgtcgtcc acgatcaaga cgcgccggcg
     4201 cccgctctcc aaggcgtcgg tggggattcc attctccttc atgaagcggt agagggcatc
     4261 ccgaggaatt cgccggaacc gtgatccggg gactcgaaac cccttgagtt gacccgagtc
     4321 aaagcagcga atgatcgtct gctgactcac tttacagacc ttggccgcct cgcctgtcgt
     4381 gaaaaccgtc ttcatcgttg tgtaggtccc atcttgcgag atcaaggacg tggtcctccc
     4441 tctcccagtt aatgg
//
LOCUS       DATAQL010001400         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1823836, whole genome shotgun sequence.
ACCESSION   DATAQL010001400 DATAQL010000000
VERSION     DATAQL010001400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823836"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VHS97_24060"
     CDS             complement(<1..1013)
                     /locus_tag="VHS97_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3451350.1"
                     /translation="MPRRLAPLAAILCAVGFWYQQPVIALDTPAMTPEAARDSIQVER
                     GFKVELVASEPLVKDPIAFEWGADGKLWVVEMGDYPLGIDGRGKPGGVVRYLEDTNND
                     GRYDKQTTFLDGLGFPTGVMPWREGVLVACAPDIFYAEDRDGDGKADRREVLYTGFVE
                     GNQQHRVNGFELGLDGWVYGANGDSGGTVRSVKARKTTQISGRDFRFRPDTGDFEAES
                     GRTQYGRHRDDWGHWFGNNNPNWGWHYVLANRDLSRNPDFAPPDTRATLEPDTRLFPI
                     SHTLARFNDPGAANHVTSANSPTPYRDDLFGAEFATSLFVSEPVHNLVHRMVLEPQGM
                     SFRG"
     gene            1217..1795
                     /locus_tag="VHS97_24065"
     CDS             1217..1795
                     /locus_tag="VHS97_24065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX3451351.1"
                     /translation="MKPRLILASASPRRRQMLANAEYDFEVDPSGIDEPDPIAEMSPW
                     AYAAHLAWRKAAEVARRRSSGLILGVDTICVVDGQILNKPIDRADAERMIRLQEGHDT
                     HVISGICLYRADRHEWLGAVEVSVVRFKSLSDAERSAYLDLERWQGKSGGYGVQDGDP
                     FVTVVRGSFSNVVGLPIERLEILVQMYSSLTL"
     gene            1882..2463
                     /locus_tag="VHS97_24070"
     CDS             1882..2463
                     /locus_tag="VHS97_24070"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX3451352.1"
                     /translation="MAPRLIGHGHYFKGMFKYAVLPLLIVGLAVSLLAPFLQWFRPAE
                     ANEAMFAPPAPINGERAFGYLKEICEIGPRIAGSEANARQRKLVADHFTKTGGTVSEQ
                     TFRAQHPVTGERLSMVNLIGSWQPDRPRRVLISAHYDTRPHPDEEVDPTRQKLPFLGA
                     NDCASGVALLMEIANHLNELTTPWGVDLVLFDG"
     gene            2596..3519
                     /locus_tag="VHS97_24075"
     CDS             2596..3519
                     /locus_tag="VHS97_24075"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX3451353.1"
                     /translation="MVGGRNLQLKQEPNSLMLACMNDAKSYAMPQKLNETMGGATVGE
                     VVESCSNLFAVGDSVRGMLGWAEYGVAKPEALSKVDARLIPLSAYLGAAGMPGMTAWY
                     GFNKILEPKAGETVVVSAAAGAVGSVVGQLARAAGIRAVGIAGGADKCRYVEEELGFD
                     ACIDYKAAADAKALAGMIAAAAPKGVDAHFENVGGDVLNAVMTLLNPFARVAICGLIA
                     DYNATPGQMMKSAIVNPAVFLVARFKMQGFIISDHLDLWPQGLKELAGLVASGKLKYK
                     ETVAEGLEAAPAAFIGLLKGHNFGKQLVRLY"
     gene            3525..>3742
                     /locus_tag="VHS97_24080"
     CDS             3525..>3742
                     /locus_tag="VHS97_24080"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3451354.1"
                     /translation="MKQLILHHYPMSPFAEKIRLILGFKNLHWSSVLIPNIMPKPDLT
                     ALTGGYRKTPVLQIGADIYCDTALIAEV"
BASE COUNT          732 a         1074 c         1173 g          763 t
ORIGIN      
        1 ccgcggaagc tcatgccctg gggctcgagc accattcgat gcaccaggtt gtgtaccggc
       61 tcactgacaa acaggctggt ggcgaattcg gcaccgaaga gatcgtcgcg atagggcgtc
      121 gggctattgg ctgaggtgac atggttggcg gcgccggggt cgttaaaccg ggccagcgta
      181 tggctgatgg gaaacagccg ggtgtcgggc tcaagtgtcg ctcgcgtatc gggtggggcg
      241 aaatccgggt tgcggctgag gtcgcgattg gcgaggacgt agtgccagcc ccaattaggg
      301 ttgttgttgc cgaaccaatg gccccagtcg tcgcggtggc gaccgtactg cgtccgcccg
      361 ctctcggctt caaaatctcc ggtgtccggt cgaaatcgga aatcacggcc gctgatctgg
      421 gtagttttgc gcgctttgac ggaacggact gtgccgccgc tgtcgccgtt ggcgccatag
      481 acccagccgt ccaaacccag ctcgaagccg ttcacgcgat gctgctggtt cccctcgaca
      541 aaaccggtat aaagcacttc gcgacgatcg gcttttccgt cgccatctcg gtcctcggcg
      601 tagaaaatat cgggagcgca ggcgaccaag acgccctcgc gccagggcat cacgccggtc
      661 ggaaagccaa gcccgtccag aaaggtcgtt tgcttgtcgt atcgcccgtc attgttagta
      721 tcttccagat accgaacaac gccgccaggt tttccacggc cgtcgattcc cagcggatag
      781 tcccccatct cgacgaccca gagcttgccg tcggcccccc attcgaacgc aatcgggtct
      841 ttgacaaggg gctcgctcgc gaccagctcc accttgaatc cgcgctcaac ctggatggaa
      901 tcacgtgccg cctcgggcgt catcgccggc gtatcaaggg cgatcacggg ctgttggtac
      961 cagaagccga ccgcacacaa gatcgcggcg agcggcgcga ggcggcgcgg catgagagca
     1021 ttccttcatg gtagtaacgg acggggcgta gcattcgcgg tcccaatatt ttggacggtc
     1081 tgtgcgggtt tgcaaacccc gttcatcagg ctgtcgggtt tacggcttgt tggcaagggg
     1141 ccagtggcga taccatcagg tttccgacca gccgccggcg ctcgggaact atcgcaagaa
     1201 tggaccggac gctcctatga aacctcgctt gatactcgcc agtgcgtcgc cgaggcggcg
     1261 gcaaatgctc gcgaatgccg aatatgattt cgaggtcgac ccttccggga tcgatgagcc
     1321 cgacccgatt gccgaaatgt ctccctgggc ctacgcggcg cacctggcgt ggcggaaggc
     1381 cgcggaggtc gccagacgcc ggtcatctgg gttgatcttg ggagttgaca cgatctgtgt
     1441 cgtcgacggt cagatactca acaagccgat cgatcgagcc gacgccgagc ggatgatccg
     1501 gcttcaagaa gggcacgata ctcatgtaat ctctggcatc tgcctgtatc gggccgatcg
     1561 ccatgagtgg ctcggggcgg tcgaggtgag cgtcgttcgt ttcaagtcgc tgagcgacgc
     1621 ggaacgtagc gcttatctcg acttagaacg ctggcaaggc aagtcagggg gttatggcgt
     1681 ccaggacgga gatccgtttg tcaccgtcgt tcgcggcagt ttctcaaacg tagttggttt
     1741 accgatagaa cgcctggaaa tactggttca gatgtattct tcgttgacgc tttgagccgg
     1801 tcatcccgcg caagtttggg acgatgagga aacgaccgtg agcaagaccg aacgggacaa
     1861 ttcaatcacg ggagtgatta gatggcacct cgcctgatcg gtcatggcca ttatttcaag
     1921 ggcatgttca agtacgcggt cttgccgctt ctgatcgtgg gcttggccgt gagcttgttg
     1981 gcccctttcc tgcagtggtt ccgacccgcg gaggccaatg aggcgatgtt cgcaccccct
     2041 gcaccgatca atggcgaacg cgcttttgga tacctcaagg agatttgcga gatcggccct
     2101 cgcatcgctg ggtcggaagc caatgcccgc cagcgcaagc tggtcgcaga ccatttcacc
     2161 aagactggtg gcacggtaag tgagcagaca ttccgtgcgc aacatcccgt gaccggggag
     2221 aggctgtcga tggtcaactt gattggctca tggcagccgg atcgcccgcg gcgggtcttg
     2281 atctccgccc actacgatac tcggccgcac ccggatgagg aggtcgaccc cactcggcaa
     2341 aagctcccgt tcttgggtgc caatgattgt gcgtcgggag tcgcgctctt gatggagatc
     2401 gccaatcact tgaacgagtt aacgacgcct tggggagtcg acttggtcct cttcgacggc
     2461 taggagttgg tttttgggaa taatccgcgc gtgggcgaat atttcctcgg ctccaaggag
     2521 tttgcgcgtg tttatgcacg gcaggtcgat cgaccacgac cgaagatgcg ctatgaggcg
     2581 gggatcttat tcgacatggt cggcggccgc aacctccagc tcaagcaaga gcctaacagc
     2641 ctaatgcttg catgcatgaa cgatgcgaaa tcttatgcga tgcctcagaa gctcaatgag
     2701 accatgggcg gcgccacggt gggtgaagtc gtcgaatcgt gcagcaatct gtttgcagtc
     2761 ggcgatagcg ttcgggggat gctgggttgg gccgaatacg gcgttgccaa gcccgaagcc
     2821 ctatccaagg tcgatgcccg gctgatcccg ctctcggcgt acttgggtgc cgccggcatg
     2881 cctggaatga ctgcctggta cggcttcaac aaaatcctcg agccgaaggc cggcgaaacc
     2941 gtggtcgtca gcgctgcggc gggcgccgtc ggcagtgtgg tcggacagct ggcgcgcgcc
     3001 gccggcattc gtgcggtagg catcgcggga ggagccgaca agtgccgtta cgtcgaagag
     3061 gaactgggat tcgacgcgtg catcgactac aaggcggctg ccgacgcgaa agcactggcg
     3121 ggaatgatcg ccgctgcggc cccgaagggc gtcgacgccc actttgaaaa tgtcggtggc
     3181 gacgtgctca atgccgtgat gacattgctc aatccgtttg cgcgagttgc gatttgcggc
     3241 cttatcgccg actacaacgc gacaccgggc cagatgatga agagcgcaat cgtgaacccc
     3301 gcggtgtttc tggtggcgcg tttcaagatg cagggcttca tcatctccga tcatctggat
     3361 ttgtggccgc aagggttgaa ggaactcgcc ggcctggtcg catccggaaa attgaaatac
     3421 aaggagaccg tcgcggaggg actcgaagcg gcgcccgccg cgttcatcgg cttgctgaag
     3481 ggccataact tcggcaagca attggtccgg ctttactaaa acttatgaaa cagctcattc
     3541 ttcatcacta tccgatgtcg cctttcgccg agaagatccg gctgatattg ggattcaaga
     3601 acctgcactg gtcctcggtg ctcattccca acatcatgcc gaagccggac ctgacggcgc
     3661 ttaccggcgg ctaccgcaag acgcccgtac tgcagatcgg cgccgacatc tactgcgaca
     3721 cggcgctgat cgccgaggtc at
//
LOCUS       DATAQL010001401         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2436317, whole genome shotgun sequence.
ACCESSION   DATAQL010001401 DATAQL010000000
VERSION     DATAQL010001401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2436317"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1751
                     /locus_tag="VHS97_24085"
     CDS             <1..>1751
                     /locus_tag="VHS97_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3451355.1"
                     /translation="SLKLAIDELRSGRADAMLCGGVSRPDPLYTQMGFSQLRALSTRG
                     KATPLDQDADGLVVAEGAGMFLLKRLDHAQAHGDRIYGVVAGIGLSNDIHGDLLAPSA
                     EGQLRAMRMAYERAGWSPNDVDLVECHAAGTPVGDAVEAESLRALWGEAGGKKHQCAI
                     GSVKSNIGHALTAAGAAGLLKLLLALKNQTLPPTANFRRPAPKLRLDESAFRVLTEPE
                     PWHRNTPNEPRRAAISGFGFGGINCHVLIEEYDSKLRNERGEFVSQPRPGPSSGLGTK
                     PAGPVAIVGLSACFGPFQGKKAFEERVLGYERAITPADPGNWWGIADSSWFKSQGWNR
                     TRFAGYYLDSLEFPLEKFRIPPRELGDMLPQQSLMLKVAADALGDARWDSRQAVRTGV
                     LIGIGLDLNTTNFHLRWSMDEQARAWNDLFDLGLSADDLARWTDELRHSAGPALTANK
                     TMGSLGGLIASRIAREFRIGGPSFTVSCDETSGVQALAIAACWLQRSELDAVIVGAVD
                     FAGDARAVITRRQLNSDPSRSNETLAAPTSGNPVVSTACDGAVALVVKRLADAERDGD
                     PIYAIIGDVATGCKRGN"
BASE COUNT          334 a          552 c          568 g          297 t
ORIGIN      
        1 actccctcaa gctggcgatc gacgagctgc ggtccggtcg ggccgatgcc atgctgtgcg
       61 gcggcgtatc tcgccccgat ccgctgtaca ctcaaatggg tttctcgcaa ctccgcgcgc
      121 tgtcgacgcg aggcaaggcc acgccgctcg atcaagacgc cgatggattg gtggtcgccg
      181 aaggggccgg gatgttcctg ctcaagcggt tggaccacgc acaagcccat ggcgatcgga
      241 tctacggcgt cgttgccggc atcggcctgt caaacgacat ccacggcgat ctcttggccc
      301 caagcgccga ggggcagctt cgcgcgatgc ggatggccta cgaacgggcc ggctggagcc
      361 cgaacgatgt cgatctggtc gaatgccacg cggcgggaac tccggtcggg gacgccgtcg
      421 aagccgagag cctgcgagca ctctggggag aagccggcgg aaaaaaacac caatgcgcga
      481 tcggctcggt caaatccaac atcggccacg cgctcacggc cgcgggtgcc gccgggctgc
      541 tcaaacttct cttggccctg aagaaccaga cactcccccc gaccgcgaat ttcaggcggc
      601 cggcaccaaa gctgcggtta gacgagagcg cattccgggt cttgaccgag cctgaaccct
      661 ggcaccggaa caccccgaac gaaccccgtc gcgcggccat cagcggattt ggctttggcg
      721 ggatcaactg tcacgtcttg atcgaggaat acgactcaaa gctccggaac gagaggggcg
      781 aatttgtcag tcagccgcgc cccggcccgt ccagcgggct gggcacaaag cctgcagggc
      841 cggtcgcgat tgtgggcctg tcggcctgtt tcggaccatt tcagggcaag aaggcgtttg
      901 aagaacgggt gctgggctac gaacgagcga ttaccccggc cgatccgggg aactggtggg
      961 gaatcgccga ctcgagttgg ttcaaaagtc agggctggaa ccgcacccgg ttcgcgggct
     1021 attacctcga ctcgctcgag ttcccgctcg aaaagtttcg gattccaccc agggaactgg
     1081 gcgacatgct gccacagcag tcgttgatgc tgaaagtcgc cgccgacgcc cttggcgacg
     1141 cgcgatggga ttcgcgccaa gccgtgcgga ctggcgtgct catcggaatc gggctcgatc
     1201 tgaacacgac caatttccac cttcgctggt caatggacga acaagcccgg gcctggaacg
     1261 atttattcga tctcgggctt tccgccgacg atctcgcgcg ctggaccgac gagctaagac
     1321 actcggccgg gcccgcgttg acagccaaca agacgatggg ctcgctcggc gggctgatcg
     1381 ccagccgaat cgcgcgtgag tttagaattg gcggcccaag tttcacggtc tcgtgcgatg
     1441 agacatcggg ggttcaggca ctggcgatcg ccgcctgctg gctgcaacgt agcgagcttg
     1501 atgccgtaat cgtgggagcg gtcgacttcg ccggcgacgc ccgcgcggtc attacccggc
     1561 gccagcttaa ttcagatccc agtcggtcaa acgaaacgct cgcggcgcct acgtccggca
     1621 atcccgtcgt ttcgaccgcc tgcgacgggg cagtggcgct ggtcgtcaaa cgcctggcgg
     1681 atgcagaacg ggacggcgat ccgatctacg cgatcatcgg cgacgtggcg accggttgta
     1741 aacgcggaaa c
//
LOCUS       DATAQL010001402         3865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3500688, whole genome shotgun sequence.
ACCESSION   DATAQL010001402 DATAQL010000000
VERSION     DATAQL010001402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500688"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VHS97_24090"
     CDS             complement(<1..213)
                     /locus_tag="VHS97_24090"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3451356.1"
                     /translation="MIESQAPARSKHSIIEWLVQWIARELGLAPVEIETDKSLLNYSL
                     SSVTAMMLVGDLEEWLGLTLPPTLVWD"
     gene            complement(337..2148)
                     /locus_tag="VHS97_24095"
     CDS             complement(337..2148)
                     /locus_tag="VHS97_24095"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3451357.1"
                     /translation="MKPIHSAVMAEPDVSRKRANELIGWLRQYAEERIDSRLFDERRC
                     VPPFVILDFGNRGVLGMQIPEEYGGLALRNRDFIRVMEQLSAIDLSLASLVFIHNANG
                     IRPIVGYATPALRAELLPLLAHGRELSAFALTEPVAGSNLPSIATVAVPDGEGGWRLR
                     GVKRWNGSGWAGVVSVFARQVDSSGRLGHATGFVVRQGMPGLRVGPESLTMGVRSIMQ
                     NSIYFDDVAVRPEHVWGEVGKGMEVADEALLVARLCMGAMSLGGMKRCAQLMHRYASR
                     RVVSTGRLLDSPITRAAFSRLAIEITLVEALVDRLVEVMDQGEYPPEEACMMVKILGS
                     DSLWAAADDLVEMLGGRGYMENNIAPQIMRDCRMLRIGEGANELMTLSVGRRVYHSEK
                     LHQFLRIQLGCPEMSDRLMEASQKIQHRCLSPAAPFGDRSTALSWAFTLIGQVAIKGV
                     RLAAAQATARQTRSPLLDRAVLWANLQFESALDQALRGNPAESFLLDSGVTSQAINGY
                     IEAIGDLEQGPPGVEEAIDPLLRRNPKGGGFPDLSHLPGNVIPEELVERPSPVSAPNS
                     DVDALTALEKRRLAAELLRRKVAGNAVREPESSAIRV"
     gene            complement(2175..>3865)
                     /locus_tag="VHS97_24100"
     CDS             complement(2175..>3865)
                     /locus_tag="VHS97_24100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX3451358.1"
                     /translation="LNDGGREPERLSYAELDREARRIAAWLQVSRAPGDRALLLFPAG
                     LDFLKAFFGCLYAGVIAIPAPPPEASRLKRTLPRLRAIAADAGVSIVISNPTIRALVD
                     DSRDNIPGLVAMEYVDIGEIPIRYADSWQSPALEADDLAYLQYTSGSTTSPKGVMISH
                     RNVVHHCSYLQRVCEYTPDSVSVTWLPYFHDYGLIEGLLEPIYNGTPGYAISPFAFLK
                     RPFNWLQAISLQRATHTQAPNFAYEQCVRRVKPEQLAQLDLGCMRSMGNGAEPINPRV
                     LDAFYRAFAPCGFRWEAFCPAYGLAETTLMVSCCSPSLTPRIGRFRADELALNRVVET
                     TDDGLATREVVSCGRIVAEFDVAIVNPDTSVRCEPDQVGEIWVSDPSVAQGYWRRDAE
                     TEATFRAYLADTGEGPFLRTGDLGFIRDGELFVTSRIKDLIIVAGANHYPQDIEWTVE
                     KCHADVRPNCVAAFSLPVEGDERLVVAAEFERGSIERPEDTAVILDAIKKAIAEEHEV
                     PVHALLLLARGSLPKTASGKIQRHGCWRLLEADSSDVLASWTSGTTRLAPPPSN"
BASE COUNT          762 a         1256 c         1164 g          683 t
ORIGIN      
        1 atcccagacc aaggtcggag ggagcgtcag acccagccat tcttccaagt cgcccaccag
       61 catcatggcg gttaccgagc tcaagctgta gttcagcaga gacttgtccg tctcgatctc
      121 cacgggagcg agtcccaact ctctggcgat ccattggacc aaccattcaa tgattgaatg
      181 cttcgaacgc gccggggcct gggattctat cattacgacg ctcctttttt cgattcgtgc
      241 tgaggtctca agtggaactg acaagctacg agaatccagc gttgcactgt atccacgtgt
      301 gcactatttg aaagaaattc acaacacaac actcaactac actctgatcg cagacgattc
      361 gggctcacgg actgcattgc cagcaacctt ccggcgcaga agctctgccg cgagccgccg
      421 cttctctagg gcggtaaggg cgtcgacatc ggaattggga gccgaaacgg gcgacggcct
      481 ctccaccaat tcctccggaa tgacgttccc tggcaagtgc gacagatcgg ggaaccctcc
      541 gcccttgggg ttgcgcctga ggaggggatc gatggcctcc tcaacgccag gcggcccttg
      601 ctcgaggtcg ccgatcgcct cgatatagcc attgatcgcc tggctagtca ctcctgagtc
      661 cagcaggaaa gactctgctg ggttaccccg gagagcctga tccaaggccg actcgaactg
      721 gagattcgcc cataggacgg cgcgatcgag cagcggcgat cgtgtttggc gcgcggtcgc
      781 ttgagcggcc gcgagccgca ctcctttgat cgcgacctga ccgatcaggg tgaacgccca
      841 cgagagtgcc gtcgatcgat cgccaaaggg cgccgcgggc gacagacacc gatgctggat
      901 cttttgagat gcctccatca agcggtcgga catctcgggg caaccaagct gaatccgcag
      961 gaactggtgc agtttctccg aatgatagac ccttcggccc acggaaaggg tcatcaattc
     1021 attggcgcct tcaccgatgc gcagcatccg gcaatcgcgc atgatctgcg gggcgatgtt
     1081 gttctccatg taccctcgcc cgccgagcat ttcgaccagg tcgtctgccg ccgcccacag
     1141 cgagtcagac cccaggatct tgaccatcat gcaagcctct tccgggggat actccccttg
     1201 gtccatcacc tcgaccagcc ggtcgacgag tgcttctacc agggtgatct cgatcgccag
     1261 ccgactgaag gccgcccgcg tgatcgggct gtcgaggagg cgccccgtag aaaccacacg
     1321 tcggctggcg tagcggtgca tgagctgcgc gcaccgcttc atccccccca ggctcatcgc
     1381 tcccatgcac aggcgggcga ccaagagcgc ctcgtcggcg acttccattc ccttacccac
     1441 ctctccccag acgtgctccg gacggaccgc cacatcatca aaatagatgc tgttctgcat
     1501 gatgctccgg acccccatgg tgagggactc cgggccgacc cgcaggccgg gcatgccctg
     1561 gcggacgaca aaccccgtcg catgtcccag ccggccgctc gagtcaacct gccgggcgaa
     1621 gacgctgacc acgccggccc agccggaacc attccagcgc ttgacccctc gcaaccgcca
     1681 gcccccctcg ccgtcgggca cggcgaccgt cgcgatgcta gggagattcg agcccgcgac
     1741 cggctcggtc agcgcaaagg ccgacagctc gcgtccatgg gctaacaagg gcagcaattc
     1801 cgctcgcaga gccggcgtcg catagccgac gatcgggcga attccgttgg cgttgtggat
     1861 gaagacgagt gacgcgaggc tcaaatcgat ggcggagagc tgctccatca cgcggataaa
     1921 atcgcggttc ctcagcgcca gtccgccata ttcctcgggg atctgcattc ccagcacgcc
     1981 ccggttgccg aagtccagaa tgacgaacgg tgggacgcac cgccgctcgt cgaacagccg
     2041 cgagtcgatg cgttcctccg catattgacg cagccaaccg atgagctcgt tggcgcgctt
     2101 gcggctgacg tcgggctcgg ccatcacggc gctatggatc ggtttcatgg atgtcaggct
     2161 cttcgggtgc cgagtcaatt cgatggtggg ggagcgagac gcgttgtgcc gctagtccag
     2221 ctcgccaaaa catccgagct gtccgcttcg agaagccgcc aacaaccgtg gcgctggatc
     2281 ttgccactgg ccgtcttcgg caggcttccg cgggccaaga ggagcaaagc gtgcacgggc
     2341 acctcatgct cctcggcgat cgccttcttg atcgcgtcca agatcaccgc ggtgtcttcg
     2401 ggccgctcga tagagccccg ctcgaattcc gcggcaacca ccagccgctc atcaccctca
     2461 accgggagcg agaaggccgc gacacaattg ggacgtacgt ccgcgtgaca tttctcaacc
     2521 gtccactcga tgtcctgggg atagtgattt gcccccgcga caatgatcag gtctttaatg
     2581 cggctggtga caaaaagctc gccgtcccgg atgaagccaa ggtcgccggt gcgtaaaaac
     2641 ggcccttctc ccgtatcggc caggtaggcg cgaaacgtgg cctcggtttc ggcgtcgcgc
     2701 cgccaatagc cctgcgcgac gctcgggtcg gagacccaga tttctccgac ttgatcgggc
     2761 tcgcagcgga cactggtatc tggattgacg atggccacgt caaattcggc gacgatccgg
     2821 ccgcagctca caacctcccg cgtggccaga ccatcgtccg ttgtttcgac gactcgattt
     2881 agggcgagct cgtccgcccg gaagcgaccg atgcgagggg tcagggacgg cgagcagcac
     2941 gaaaccatga gcgtcgtctc cgccagaccg tacgcgggac aaaaggcctc ccagcggaaa
     3001 ccgcagggag caaaggcccg gtagaacgca tcaagcaccc tggggttgat cggctcggcg
     3061 ccgttgccca tggagcgcat acagccgagg tcgagctggg caagctgttc gggcttgacg
     3121 cgtcgcacgc actgttcata ggcaaaattc ggcgcttgag tgtgggttgc ccgctgaagt
     3181 gagatcgcct ggagccagtt aaagggccgc ttgaggaagg caaagggcga tatcgcataa
     3241 ccgggggtcc cgttgtagat cggctccaag agcccctcga tcagcccgta gtcgtggaaa
     3301 tacggcagcc aggtcacact gacactatcg ggcgtgtact cgcaaaccct ctgaaggtaa
     3361 ctgcaatgat ggacgacgtt gcggtggctg atcatgactc ccttgggact cgtcgtcgaa
     3421 cccgaggtgt actggagata ggccaggtcg tcggcttcga gcgcgggcga ctgccacgaa
     3481 tccgcgtagc gaatcgggat ctccccgatg tcgacgtact ccatcgccac cagcccgggg
     3541 atattgtcgc gggagtcgtc cacgagcgcc ctgatcgtcg gattggagat cacgatcgag
     3601 accccggcat ccgcggcgat cgcccgaagt cgcggcagcg tccgcttgag ccggctggcc
     3661 tccggaggag gcgcggggat ggcgatcacc cccgcgtaga ggcagccgaa aaacgccttg
     3721 aggaaatcca acccggccgg aaagagcaac agcgcacggt cccccggggc cctggacacc
     3781 tgcagccacg ccgcgatccg acgtgcctca cggtccagct cggcgtagga gagtcgttct
     3841 ggctcacgcc ctccatcatt cagga
//
LOCUS       DATAQL010001403         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_755372, whole genome shotgun sequence.
ACCESSION   DATAQL010001403 DATAQL010000000
VERSION     DATAQL010001403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755372"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VHS97_24105"
     CDS             <1..196
                     /locus_tag="VHS97_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX3451359.1"
                     /translation="RLNALPYTNTCIDCQRENERVGQSRVLEPDSKRWAKVYEKSEEG
                     ETDSKINLSDFEMDFSETGR"
     gene            526..2100
                     /locus_tag="VHS97_24110"
     CDS             526..2100
                     /locus_tag="VHS97_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3451360.1"
                     /translation="MLSRSSAVIISLTSLSAGIVLGILLGRGGPVVSAQVAGSGIGGP
                     VISISGSQTKTLSRQKLADDATYDVLAKQYEQFQQINRTFELVAKAVSPSVVHIVAQK
                     TLRFEKTGKARQIEETGSGVIIRSDRVPGLFVLTNHHVVDGSEPGKIRVLLSDSRSIS
                     VGKVWTDSKADIAVLKLDRDDLPAARLGNSDDAAIGTWVMALGSPFGLRHSVSQGIIS
                     ARGRQMDELPDVVNQDFIQTDAAINPGNSGGPLVNMKGEVIAINNSIASNGGGNEGVG
                     FSIPINLARWIMNELIVHGRVTRGALGVDLHPEFPAERAISMGMDRPRGAWIDVVHES
                     TPAAKAGLLAGDVVLQFAGVNVIDLNHLINMVSMAPVGEPAEIVIWRDRRELKLNVTV
                     DERERTLKQFTSALAGGPVIDSSGLLHRPNRPNATSNFALGLELSTLNPQLAQRFGIP
                     ESWRGVLVLNVDPDSPLARFVQPADVISAIDNEAIRSSEQAVKLLNKRADHVQSIISL
                     DRPVNGDMQKHTVRLP"
     gene            2100..3164
                     /gene="trpD"
                     /locus_tag="VHS97_24115"
     CDS             2100..3164
                     /gene="trpD"
                     /locus_tag="VHS97_24115"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX3451361.1"
                     /translation="MNPLSAALVTLAGGNSLSSGESCAAVGTLLDGGAPDAIVAAFLT
                     ALRLKGETADELEGAVTAVRERMIRWESGMNALPLLDTCGTGGDGADTVNISTAAAIV
                     VAACGVSVVKHGNRAATSRSGSSDVLSALGVAADLEPGLSHRCLAELKIAFLFAPRFH
                     AGLACVAPVRRQLPFRTIFNLLGPLCNPASPTHQLVGVPNEMQAELLAQVIARQPHIG
                     RAAVVTGWSGLDEVTLEGPTQVRLIESGRVRREEWEPQDFGLSRHAVAAIKVRDPVES
                     AARLTSAFDGEKGPVRDYILANSAAALWVTGVSSLREGTDRAASAIDSGAAARLLDRW
                     RELAPASLAGGAPSTLTGGP"
     gene            complement(3142..3648)
                     /locus_tag="VHS97_24120"
     CDS             complement(3142..3648)
                     /locus_tag="VHS97_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3451362.1"
                     /translation="MNPLPPTVLIRDAVLADGPTIVEFSRRLALETEDKVLDRATLEA
                     GVATALADPDRLRFWIAEIGDLPRIVGQAAITREWTDWRNGWLWWLQSVYVVESERGR
                     GVFRALFQHIRQQAQANPDVIGLRLYVENGNQAAMRTYRSLGMKPGGYSVYEQLWLER
                     SLKDRPST"
     gene            complement(3645..4586)
                     /locus_tag="VHS97_24125"
     CDS             complement(3645..4586)
                     /locus_tag="VHS97_24125"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3451363.1"
                     /translation="MGIYDREYYRGETGGTGLFSGVSPVTKSIIVINAAVFLLDRLIN
                     PDASQQSFIDTHFAATPNGILHHFRIWQLLTATFLHVGIWHLLGNMWFFWIVGREMES
                     LYGSRDFLIFYLGAAIFSTLAWVLITAASSRNPNVPMMGASGAVMAVVTLYTLFYPRR
                     EILLFFIPMPMWLLLAIYLFVPLLPVIAGGATDVAVQSHLAGAGFGFLFKQFDLRFSR
                     LFSGRVFKPRLRIFSPAPREQTRTRSPNSSRTPASVGSGGKSAQVSVLPEEQLDARLD
                     EVLAKIAREGRAGLTEEEFRVLQEASRRARIRRSDRI"
     gene            complement(4814..5197)
                     /locus_tag="VHS97_24130"
     CDS             complement(4814..5197)
                     /locus_tag="VHS97_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3451364.1"
                     /translation="MCAKQIPSNDEAFTIERHGDLTIITATRSLEAIEFGLEEQVADM
                     VLKPLRGQENPLIVFDLSQADNFGSMFLALLIRCWKLALSQGGTMALSGVTDRTRELL
                     RVTALDMVWPMYESRREAMEALLLD"
BASE COUNT         1145 a         1634 c         1612 g         1062 t
ORIGIN      
        1 acggttaaac gcgctgccat acacgaatac ttgcatcgac tgtcaacgcg agaacgagcg
       61 cgttgggcag tccagggtac ttgagcccga ctccaaacgt tgggccaaag tctacgagaa
      121 gtcggaggaa ggcgagaccg actcgaagat caatctcagc gacttcgaga tggatttcag
      181 cgaaactggc cgctgacgga cagccctgtc gcccaagcaa tgagcattta gttgccgccg
      241 gagatcatcc ggcggctttt ttttgtccac atgcccacat ggcaatagtt tgccgaacga
      301 gcatttgctg ctttttttga aattaggtca aggcgggaat gtattcaaaa atgcacgcaa
      361 tgtttgacgt taagcaactt cggcgcctgc gcgcggtcga tctttgactt ggctgcctga
      421 taggcagtat attagaaaga cgcccaccgt ccgtaggaga aggatcttca aattaccaag
      481 cggatcttcg tccctttgat ccgccgttgg cgtggagtct ggttgatgct ttcccgatct
      541 tccgcagtca ttatctctct gacttcgctg agtgcgggaa tcgtgctggg aattctcctg
      601 ggacggggcg gccctgttgt ctcggctcaa gttgccggct cgggaattgg cgggccggta
      661 atctcgatat ctggctcaca gacgaaaacc ttgtctcgtc aaaagcttgc agacgacgcc
      721 acttatgatg tcctagccaa acagtacgag caatttcagc agatcaatcg gacgttcgag
      781 cttgtcgcca aggccgtttc gccctccgtt gtgcacatcg ttgctcaaaa gacgttgcgg
      841 ttcgagaaaa ctgggaaggc ccgccagatc gaggaaaccg ggtcgggtgt catcatacgt
      901 agcgatcgcg tccccggcct cttcgtgttg accaatcacc acgttgtcga tggctcggaa
      961 ccgggcaaaa ttcgcgtgct cttgagcgat tcgcgctcga tttcggttgg caaagtctgg
     1021 accgacagta aggccgacat cgcggttttg aagctcgacc gcgacgatct gccggcggcg
     1081 cgattgggca acagtgacga cgccgcgatt ggaacgtggg tcatggcact cggcagccca
     1141 tttggcctga gacattcggt cagccaggga atcatcagtg ctcgcgggcg tcagatggac
     1201 gagctcccgg acgtggtaaa tcaagacttc attcagacgg acgccgcgat caaccccggc
     1261 aactcggggg gcccccttgt caacatgaag ggggaagtga tcgccatcaa caactcaatc
     1321 gcatcgaatg gcggcggcaa cgaaggtgtg ggctttagca tccccatcaa cctggcgcgc
     1381 tggatcatga acgagctgat cgttcacggc cgcgtcacac gcggcgcgct cggcgtggat
     1441 ctacacccgg aatttcctgc ggaacgagcg atctcgatgg gcatggaccg accgaggggc
     1501 gcctggatcg atgtcgtcca tgaatcgaca ccggccgcca aggcgggtct gctcgccggc
     1561 gacgtcgttc tccagttcgc gggagtgaat gtgatcgatc tgaatcacct gatcaatatg
     1621 gtctcgatgg ccccggtcgg agagcccgcc gaaatcgtga tttggcgcga tcgacgcgag
     1681 ctcaagctca atgtaaccgt ggacgaacgc gaacgcacct tgaaacagtt tacctcggca
     1741 cttgcagggg ggccggtgat tgattcgtcg ggcttgctac atcgccccaa tcgccccaac
     1801 gccacgtcga attttgcact cggtctggaa ctgtcgacgc tgaatcctca gctcgctcag
     1861 cgattcggca ttcccgaaag ctggcgcggc gtactggtct tgaatgtcga tccggatagc
     1921 cctctggccc gtttcgtcca gccggcagat gtgatctcgg cgattgataa cgaggcgatc
     1981 cggtcgtctg agcaagccgt caaattattg aacaagaggg ccgaccacgt ccagtcgatc
     2041 atcagcctcg accggccggt caacggcgat atgcaaaagc atacggtccg attgccgtga
     2101 tgaacccgct gtccgcggcc ctcgtgacgc tcgctggcgg caacagcctt tccagcggtg
     2161 agagctgtgc cgccgtcgga acactgctgg atggtggagc gcccgatgcc atcgttgccg
     2221 cgttcctgac cgctctgcgg ttaaaggggg agaccgccga tgagctcgag ggtgccgtca
     2281 cagccgtccg cgaacgcatg atccgctggg aaagcgggat gaacgccctc ccactgctcg
     2341 atacctgcgg caccgggggt gatggcgccg acacggtcaa catctcaacc gccgcggcaa
     2401 tcgtcgtggc cgcctgcggc gtctcggtcg tcaaacacgg caaccgcgcg gccactagcc
     2461 gctcgggcag ctctgatgtt ctctcagccc tgggtgtggc ggctgacctc gagccgggtt
     2521 tgtcgcaccg ctgcctggcc gagctgaaga tcgcgttcct tttcgccccc aggtttcatg
     2581 caggcctggc gtgtgtggca cccgtccggc gtcagctccc cttccggacg atcttcaacc
     2641 tgctcggccc gctctgcaac ccggccagcc cgactcacca actagtcggt gtgcccaacg
     2701 agatgcaggc ggagcttctc gcacaagtca tcgcacgaca gccgcatatc gggcgcgccg
     2761 cggtggtcac ggggtggagc ggattggacg aggtgacact cgaaggcccg actcaggttc
     2821 gcttaatcga gtcgggacgg gttcgccgcg aggaatggga gccgcaggac ttcgggcttt
     2881 cacgccacgc cgtcgccgcg atcaaggtcc gcgatcccgt tgaaagcgcg gccagactca
     2941 cgtccgcctt cgacggagaa aaagggccgg tgcgagacta catccttgcc aacagtgcgg
     3001 ctgctctgtg ggtgaccgga gttagctcgt tgcgcgaggg gacggatcgt gccgcatccg
     3061 cgatcgattc gggggcggcc gcccgactcc tcgatcgatg gcgagagctc gcacccgcgt
     3121 cgttggccgg aggggctccc tctacgttga cgggcggtcc ttgagagagc gctcgagcca
     3181 taactgctcg tagaccgaat agcccccggg tttcataccc agcgatcgat aggttcgcat
     3241 cgcggcttga ttgccgtttt ccacgtagag tcgcagaccg atcacatcgg gattcgcctg
     3301 tgcctgctgt cggatatgtt gaaagagagc gcgaaacaca ccacggccac gttcagactc
     3361 tacgacatag acgctttgga gccaccacaa ccagccgtta cgccagtcag tccattcccg
     3421 cgtgattgcg gcctgaccaa cgatgcgcgg aaggtcaccg atctcggcga tccagaaccg
     3481 cagccgatcc ggatcggcca aggccgttgc gactccagcc tcgagtgtcg cccggtcaag
     3541 gaccttgtct tcggtctcga gagccaaacg ccggctgaac tcgacgatcg tgggaccgtc
     3601 ggcgagcact gcgtcacgga tcagcaccgt cggcggcagt gggttcaaat gcgatcgctc
     3661 cggcggatac gggcgcgacg gcttgcctct tgaaggacgc ggaactcttc ctcggtcagg
     3721 ccggcgcgcc cttcgcgtgc gattttggcc agtacctcat cgagccgcgc atcgagctgt
     3781 tcctcgggca gcacgctaac ctgggcggac ttgccgccac tccctacact cgccggggtt
     3841 cgcgagctat tcggcgatcg cgttcgggtc tgttcgcggg gcgctggcga aaagattcgg
     3901 agccgcggtt tgaagacgcg ccccgaaaat agtcgcgaga agcgtaaatc gaattgtttg
     3961 aagagaaatc caaagcctgc tccggccaga tgcgactgga cggcgacgtc tgtggcaccg
     4021 ccggcaataa ccggcagcag cggtacaaac aaatagatcg ccagcaagag ccacatgggc
     4081 atcgggataa agaaaaggag gatttcgcgc cgggggtaaa acagcgtgta gagtgtcacc
     4141 accgccatga cggccccgga ggctcccatc atagggacgt ttggattgcg gctcgacgca
     4201 gccgtaatca agacccaggc caaggtgcta aaaattgccg cgcccaggta aaagatcagg
     4261 aaatcgcggc tgccgtacaa tgactccatt tccctgccga cgatccagaa aaaccacata
     4321 ttgcccagca agtgccagat gccaacgtgc aggaacgtcg ccgtcagcaa ctgccagatg
     4381 cgaaaatggt ggagaattcc attgggtgtc gccgcgaagt gggtgtcaat gaacgattgt
     4441 tgactggcgt ctgggttgat caggcggtcc agtaaaaaca cggcggcatt gatcacgatg
     4501 atggacttgg tgaccggcga aacgccgcta aacaagccgg taccaccggt ttcgccacga
     4561 taatactcgc ggtcgtagat gcccatgacg gctccgctcc ggcggaacga aatccagacc
     4621 tgtgaacgat ggtcgagacc gatccgaggt cgtctgcctg ttcatcatcg gactcgccga
     4681 cgaagctgtc aacggcccgc gccttcatgc tcttcgacga gtcgccggct agagcgacct
     4741 gtgcaccgcg aattggtcaa ttggggacgg caagaccgtg cgcgtctcgg ggttcgcgga
     4801 cgattcgacc tactcaatcc agcagcaaag cttccatcgc ctcgcgcctg gattcgtaca
     4861 tcggccacac catatcgagc gcggtcaccc gcagcaattc ccgtgtccga tcggtgaccc
     4921 cggaaagggc catcgttcct ccctgcgaca acgcaagctt ccagcaacgg atcaagagcg
     4981 ccaggaacat cgagccgaag ttgtcggcct ggctcaaatc aaagacgatc agcggatttt
     5041 cctgtccccg caacggcttc aaaaccatat ccgcgacctg ctcctccagt ccgaattcaa
     5101 tggcttccag cgaacgcgtc gccgtgatga tggtcaaatc gccatgacgc tcaatagtga
     5161 acgcctcatc attcgaaggt atttgcttgg cgcacatcag gttggctcaa aatccgcggg
     5221 agcgggattt tcgtcactcg acacaaaact gcggtacctc agttcattac cttagaggtt
     5281 tacgatcgac tagacaagcc ccccccactt atgaagccga cggacgagag ttcgagaacg
     5341 cgcgcacgac ctcgtaaagg tcgttactga ctaaaagttc atcgtcctcg aaatcgcgta
     5401 gccaaacgta attgtcacga tacgcctgag ccaagagcgg ataaagctca ctc
//
LOCUS       DATAQL010001404        14632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1975692, whole genome shotgun sequence.
ACCESSION   DATAQL010001404 DATAQL010000000
VERSION     DATAQL010001404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 14632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14632
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975692"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="VHS97_24135"
     CDS             <1..1754
                     /locus_tag="VHS97_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451365.1"
                     /translation="TAEVSRILAAPDADPAAVIAIQPDVIGPTSEIAVSQIELTQDAF
                     TESLEAAIAAHEIAALGQPERFDQPAPANLTEPEAPADTPAAAEQTDEPPMRAEPTPI
                     KPNEDAPESAVRRSRPSSRLTDDTPAEPDPRRHRQASSRGKSGLGARREASHRTGMDR
                     DFRPTEQYPPDPRSAAKPEKLPSAKDILATHCSRPRPQATPVQAVRKTPRDAPTPARE
                     PAHWSLPAWVAGPPAAVFVMTAGLASCTLSWLWADESHSASIMTARLLAANRIVQRGP
                     LPESCAPPAGSWTRSTAQHLAHWAIFMSLAEPGQEPSPQQVRSLLNSALAVSPLNPTA
                     RLALAQLDQASDARAISTRGLGHSRDAISLSWCARRLLATGHKQDALRMYAAALNLAI
                     KRGPVRVAPPQYSNDPQAPRYLLPGEECVRDVVVELLSKRPWTFDEWSAALPKSSMAF
                     VAAARVLREQSLSEAQVLIDRVMDLREPLDAVPAARALTLAARAEACALQSGWKQAEE
                     QYRLAIEVCEDETIKRSWWFNLADIEQRLDDEGQRQIALRAAAVPSGDDIARRAADIQ
                     RANIAPSFSRFNGTKAN"
     gene            1784..2794
                     /locus_tag="VHS97_24140"
     CDS             1784..2794
                     /locus_tag="VHS97_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEX3451366.1"
                     /translation="MLVTNASRPPTPNSNHESESLVQLLLRSLIDPGQRKFWIGALVS
                     VGLFVRLFWDNLYHFYYVWTTDENYSHGFLVPLISLYFANQVARRGPVSVSGGQWCGG
                     LLLVTALLVRLVTIPLPIPFLGDIAFLVGLAGLFTILCGTTAFRRYWFAFFFLIFMVP
                     LPIALYSRIASPLQLLASRVASTVMNATGVPVLCEGNRMTLPGGLQMFVAEACSGMRQ
                     LTGFLALTTAVAYLSTRPIWYRVIIVVSALPIALSANIARVMLTGYIMHFVNPQFALG
                     TYHTLEGLVMMAFGLALLHCECCILDLIFRMAAGESEASAGAAHLTRGRTQSARLEPR
                     GM"
     gene            2843..3517
                     /locus_tag="VHS97_24145"
     CDS             2843..3517
                     /locus_tag="VHS97_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEX3451367.1"
                     /translation="MSNLVRTMISGAILATGLAAQAGLERLNQTVRPPLLRPLHSIPL
                     VMGDWVGRDEPVNADIVDRSQTTEYLNRVYESRKQPGVRLRVWINYSNLGNNLRHTPE
                     ICLPSGGWTKIESMTRVIDVPLDSERSMEVTRLAYARGELVEHVGFWYYIFGEGKLEN
                     YVRRLPITSRSSHGQTTRGSSMTVEVFYPGDNDPDGDALREFADELVKSLEPILPLER
                     AIYHIP"
     gene            3554..4570
                     /gene="gmd"
                     /locus_tag="VHS97_24150"
     CDS             3554..4570
                     /gene="gmd"
                     /locus_tag="VHS97_24150"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX3451368.1"
                     /translation="MKRALITGITGQDGSYLAEMLLGKMDYEVHGLVRRSSSLNRQRI
                     DHFFARDPSVHERFHLHYADLADASSLAMVMEEVRPDEVYNLGAQSHVRVSFDQPLYT
                     ADVVGLGTLRLLEAVRHLSKSKSVKFYQASSSEMYGSAPPPQGPNTPFHPRSPYACAK
                     LYGHWQTINYREAYGLFACSGILFNHESPRRGESFVTRKVTLGATRIKEGLQKKLVMG
                     NLDSKRDWGFAGDYVRAMWLMLQQDKPEDYVVATGETHSIHELLDVAFSHLQLDYRDF
                     IEFNTKYTRPSEVDVLLGDATKARESLGWTPEVDFKGLVTMMVEHDLELARREKFAKS
                     YRGG"
     gene            4628..5986
                     /locus_tag="VHS97_24155"
     CDS             4628..5986
                     /locus_tag="VHS97_24155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3451369.1"
                     /translation="MEKRRFADSVLVACPDARPPAYQAVVGLCRARLLRSFVTSTYYD
                     RDGHLASIMRRLAPHALARLEGALLRRHEPEIPAALVRTLPSFDVALRIEARMEATHP
                     RITRALARWRTDQFDRRLARTVGRSRLGAVLLFSDVGSGATLPLCRRLGIPSIVSMVH
                     GDVRDEERVMETESAIAPEFMGMYLGGGSLDRKLLAWLHERRLRDLAVADRILVPSDH
                     IAHRLTQQGTALDRLHVIPYAADCRRFGPGSEKRDPDTCTFLFAGGISQRKGIKYLLE
                     AWQRIRRPGWRLQLLGPLPSKPGPLGPYLESVEPLGRVSHAEMPARMREADVFVFPSL
                     FEGSAVVTYEALASGLPCVVTAEAGSVVRDGLEGFVVDARDVATLAARMETLARDRDL
                     RACMAGAARARALAFDWPRYHRAIVAVVNDLIGQENDLALTRPDGRHARGFVPARAFS
                     RS"
     gene            6013..7125
                     /locus_tag="VHS97_24160"
     CDS             6013..7125
                     /locus_tag="VHS97_24160"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3451370.1"
                     /translation="MKRIKELDSIRGLAALVIVFYHLWFQNIGILGAAVDLFFVLSGY
                     LITTIILANTFSERFLFSFYMRRGLRIWPIYYLTLVAMVLIYAYLPPCGNLNDLPYYL
                     TFTQEVVHYRPSMEPSFPSAFRHTWSLAIEEQFYLIWPPLVWWLGSKRLPIASMLLVV
                     VAVFARALDFSAFLLITHCDGLALGGLLAGILAAHEGSVTGSRKYRLGMGILGVVSTV
                     ATIAVIALPGCLNSQWPGLVPDVLVRVIKPLCLNLVFFAVVGNIVSYAGLPGLGWLRD
                     SRLVYLGSISYGIYLYHHIIFEVFAYYASFYGWNDSVVIDFGKVALSIALAALSWRFV
                     EQPILAFKDRFSYREATRAVLEIGGAVDDIRGVQAG"
     gene            7132..8487
                     /locus_tag="VHS97_24165"
     CDS             7132..8487
                     /locus_tag="VHS97_24165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451371.1"
                     /translation="MDDLYSVYATGGFILLYFLIQVARGSFDPFAPVWLFFVGFLQVY
                     VLQAISYHEWAIEIRGKDLVAAANFRAFWALSWFLIVYQFGPGRMFSALLPDPPRGWS
                     TAVVSALSPPLILWGLFCAGMVLRSGVDAQETVSAEGTLLLSFPFVMMVAGVLLIVTG
                     RSLANPRPQFLTAGLMVSGAYVLIWMFNGKRSHSLIGVLATVCAFYITRLKRPSWPVL
                     LATGFTGSMVVAIAIGWRGNLDYERSFTGFISYLGDFKVNKVLESLNVSEGDDEEESR
                     SHETAEYGGFLLMMDTVPQKSGYDHGQNYIRVFSTFIPRLLWPSKPLYGRAEWVSAWI
                     AGSELERDEEFTGPAIGILGATHLNGGAAGTLIVLACVALSLRTAYEYFRAHADVPWV
                     QFWWSITFYNSWFMVVGDDPMTWFYYNWGFTTFPIVIVVWLANQALARTQPGRLASAA
                     A"
     gene            8542..9771
                     /locus_tag="VHS97_24170"
     CDS             8542..9771
                     /locus_tag="VHS97_24170"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3451372.1"
                     /translation="MKTAWSLDSITKSLRSFPLTANEKIDICRGVFAFLVVAAHAVDL
                     SWSIHPDAPARFSWWLHRFLLYVVAAGVYWVIGFFVISGYCIQLSVSRSISENSFPLT
                     RYLAARFTRILPLYYLALAFAVVVEWLIAGARPPYWSNGINGSVLFAQIFILQNLSQT
                     FGSFAPSWSITNEMFYYLFYGALVCFALKRGIRAVKLGMIVCLAVAVPMDLLYFGWIR
                     SRFVANVGLLFGLGTFWFLGAFVAEYRDTLRCSHLARSVSRFWPLLLAAAIAMWFSQR
                     VHILVVYVVLAVAFTVMLIRIVAADKGSPGRDERRRPSRLVEMLGLASYPTYLFHGPL
                     LFLVGSAILRWKFVSDWRLTWLILVSVGISTGIALGYLAERPIMNRRAAFLSRLRSQR
                     TPPARGDVKVPVLSIQQ"
     gene            9795..10994
                     /locus_tag="VHS97_24175"
     CDS             9795..10994
                     /locus_tag="VHS97_24175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451373.1"
                     /translation="MTVAICFTNFGPYHLARLRALAARLGTTGIRLVAYEVAGREQAY
                     PWSPDRNIEPFEWITLFPDRVLETIAPADCRRAMAVALDRDQPDVLCLVGYARPESMA
                     AASWAKRHGRPAILLSESQAIDRPRIWWKELIKMRRTRQFDAALVGGPAHRDYLVRLG
                     MPPERIALGYNAVDNGYFTTEAQRWRNSATGRAGLPAAPYFLTVCRFAPEKNLVRLIR
                     AFARYREESNPQSAWELVLCGDGPGSAEVEGAIVRSGCAAAIHRPGFLQTDALTRWYG
                     HAEAFVLPSLSEPWGLVVNEAAASGLPLLVSARAGCAATLVPEPEGTTGGQFDPLDVE
                     ELTTKLAWVASLTAEDRRAMGRRAAQVVSDWGPDRFAHGVIEAIELARAKTQWPARTA
                     LTATSRS"
     gene            10999..12222
                     /locus_tag="VHS97_24180"
     CDS             10999..12222
                     /locus_tag="VHS97_24180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451374.1"
                     /translation="MIATRPNNNVALAINAPPGRRGSPHAEVNPATGWLHLCNGLDPV
                     RDGGMVPSILGMTEALSRLKGEVTIVTPTRSRLDQTQLGNGLTLKGPETDLEAAVGSA
                     EIVHMHGLWQAHTRRGARAARAARVPYLIAAHGMAEPWALRHKRWKKRVYMALVEAKN
                     LRQASCLHALARPEIAHLRSIAPRTPICFVPNGVDLAPFENMPPRSVLEAQYPELKGK
                     FVLLFFGRVHVKKGLDLLAEALYRTIPDHRALHLVVAGTDDGAWSPFRDRIASLGLTD
                     SMTYLGHVAAERARQVWGAADAFVLPSYSEGFSMAVLEALACSLPCLVTTACHFPELA
                     AAGGGIEVAATAESVTQGLRDLVERTPAERARLGQVGRRLVEENYTWDRQAERLASVY
                     AWLKGGGPPPEPVVP"
     gene            12313..13254
                     /locus_tag="VHS97_24185"
     CDS             12313..13254
                     /locus_tag="VHS97_24185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3451375.1"
                     /translation="MGKTPVSVIIPVRNEAENLERCLPALAWADEVFVVDSQSSDQTA
                     EVAERSGASVVQFHFNGAYPKKKNWALDNLPFRNEWVLIVDADEVVIPELATEIAGRI
                     ARDEADGFYLNSQYYFLGRRIRHCGYASCWNLRLFKHRLGRYERMPDVTGGRTGDNEA
                     HEHVELEGRVLRLNHELEHHAYPTVAIWVEKHNRYAIWEAALAERLIHEPIPNSIGSV
                     QRYKRRLKKIAWRLPLRPLVRFLYAYVARLGFLDGKPGLYFCGLLAFYDFLAMANRYE
                     RQLAETGATPGVAAGFPGSRRQALLDSRLPNSRIRVF"
     gene            13363..14118
                     /locus_tag="VHS97_24190"
     CDS             13363..14118
                     /locus_tag="VHS97_24190"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX3451376.1"
                     /translation="MRGIIENFTRDWVIRRRLSADFSSAPIHVTPSAGLRYLFRPMNS
                     VDPSLLELVKEFIAPGAVVWDIGANIGLFSFAAASIAGPEGLVVALEPDAWLVQLLRR
                     SALEQPAGSASVQVVPAAVASEVSLRTLCLANRSRAANYLAEFGTTQTGGSREQQSVV
                     AVTLDWLMDSLPAPSVLKIDVEGAELEVLKGAHRLFETVRPVVFCEVIPASEPGVTDF
                     FNEHNYQIFNGELPSGERQPLSASPWCTVAIPA"
     gene            complement(14285..>14632)
                     /locus_tag="VHS97_24195"
     CDS             complement(14285..>14632)
                     /locus_tag="VHS97_24195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451377.1"
                     /translation="NPGGWSQLERSNRHDEFDRNPGGWRQLKRSNRYDDLDGHPGGIG
                     RFECADDNWNDLRDNDGQYRDEPDRRDHAHTFVAGSTGFDFNVYKRQHRRRRFRLRQF
                     IRADFDLIVGEFQ"
BASE COUNT         2583 a         4557 c         4459 g         3033 t
ORIGIN      
        1 caactgcgga agtctcgagg attctggccg ctcctgatgc cgaccctgcg gctgtgatcg
       61 cgatccagcc cgatgtcatc ggccccacgt cagagatcgc cgttagccaa atcgagctaa
      121 cccaagacgc ctttaccgag agcctcgagg ccgccatcgc tgcccatgaa atcgcggcac
      181 tcgggcaacc agagcgtttc gaccaaccag ctcccgccaa cctgaccgag cccgaggcac
      241 cggcggacac gcctgctgcc gccgagcaaa cggacgagcc gccgatgcgg gcggagccca
      301 cgccgatcaa gccgaacgaa gacgcgccgg aatcggccgt caggcgaagc cgcccctcgt
      361 cccgcctcac ggacgatacg cccgccgagc ccgacccacg ccggcacagg caggcatcga
      421 gccggggcaa gagcggcctg ggagcacgaa gggaagcatc ccaccgaact gggatggaca
      481 gggatttccg gccaacagag cagtatccgc ctgatccgcg ctccgccgcc aaaccggaaa
      541 aactgccatc cgccaaggac attctggcga cacattgcag tcgaccacgg ccccaggcga
      601 cgccagtgca ggcagtgcgc aagacaccaa gagatgcgcc caccccggca cgcgagcccg
      661 cccattggtc gttgccagcc tgggtcgccg gtccgccggc cgcggtgttt gtcatgaccg
      721 ccggcctcgc gagctgcacc ctctcgtggt tgtgggcgga cgagtcgcat tcggcatcga
      781 tcatgaccgc acgattgttg gcggctaacc gcatagtcca gcgcggaccg ctcccggaat
      841 catgcgcccc gcccgcgggc tcatggactc gctcgaccgc gcagcacctg gcacactggg
      901 cgatcttcat gagtctcgcc gaacccggac aggaaccctc gccgcagcaa gtccgatcgc
      961 tcttgaatag tgcgctcgcg gtttcgccgc tcaatccgac ggcccggctg gcgctggcac
     1021 agcttgacca agcaagcgac gctagggcga tctccacgcg cgggctgggc cactctcgcg
     1081 acgcgatcag cctctcgtgg tgcgcccgtc ggcttttggc gaccggccac aagcaagacg
     1141 ccctcagaat gtatgccgcg gccttgaact tggccatcaa gcgtgggccc gtccgagtag
     1201 ccccgcctca gtacagcaac gacccccagg ccccacgcta cctgttaccc ggcgaggagt
     1261 gtgtgcgtga cgtcgttgtc gagctcttat caaaacgccc atggacgttt gatgagtggt
     1321 cagcggctct tcctaagagc agcatggctt tcgtggcagc cgctcgcgtc ttgcgcgagc
     1381 aaagtctttc cgaagcgcaa gtgctgatcg accgggtcat ggatcttcgc gagccgctcg
     1441 acgccgtccc ggcagcaagg gccttgactc tggccgcgcg tgccgaagcc tgcgcgctcc
     1501 agtcaggctg gaaacaggcc gaagagcagt atcgcctggc gatcgaggtc tgcgaggacg
     1561 agacgatcaa gcggtcgtgg tggttcaacc tggcggacat cgagcagcgg ctcgacgacg
     1621 aagggcagcg acagatcgcg ctgcgggccg ccgccgtccc atccggtgac gacatcgcgc
     1681 gccgagccgc cgacatccag cgagccaaca tcgctccatc attctcgcgg tttaatggaa
     1741 caaaggctaa ttgatcataa gtcgaaatga agggaaacga tccatgttgg tgacaaacgc
     1801 gtcgcgtccc ccgacgccga atagcaatca cgagagtgaa agcctggtgc agttgctctt
     1861 acgcagcctt attgatcccg ggcaacgaaa attctggatt ggcgccctgg tcagcgtcgg
     1921 actattcgtt cgtttgttct gggacaacct gtaccatttc tactatgtct ggaccaccga
     1981 cgaaaactac agccacggct tcctcgtacc actgatcagc ctctatttcg ccaatcaagt
     2041 cgcgcgccgg gggccggtgt ccgtttcggg aggtcagtgg tgtggcggat tgttgctcgt
     2101 gaccgcgctc ctggtccggc tcgtcaccat tcccctgccg atcccgtttc taggggacat
     2161 tgcgttcctg gtcggcctgg cgggtctgtt tacgattctt tgtggaacga cggccttccg
     2221 gcgttactgg ttcgccttct ttttcctgat tttcatggtg ccgttgccaa tcgcgctcta
     2281 ttccaggatc gcctcaccac tccagttgct ggccagccgc gtggcgtcaa cggtcatgaa
     2341 tgccacgggc gttcccgttt tgtgtgaggg gaaccgaatg acgctgccgg gcggccttca
     2401 gatgtttgtc gccgaggcgt gtagtggcat gaggcaattg acaggctttc tggcactgac
     2461 caccgctgta gcctacctgt ctacgcgccc aatatggtac cgcgtcatca ttgtagtctc
     2521 ggcattgcca atcgcccttt cggccaatat cgcgcgggtg atgctcaccg gctacatcat
     2581 gcatttcgtc aacccacagt ttgccctggg gacgtaccat acactcgagg gtttggtgat
     2641 gatggcgttt ggactggcat tgcttcattg cgaatgttgc atactcgacc tgatctttcg
     2701 catggccgcg ggagagtcgg aagcaagtgc gggtgcggcc catcttacgc gcggccgcac
     2761 gcagtcggct cgccttgaac cgcgcgggat gtagggaaaa cacagccaat ccgcacagag
     2821 atcgaggttg caaggaggga ccatgtcgaa cttggtaagg accatgatca gcggtgcgat
     2881 tctggcaacg gggctggccg cccaggcggg ccttgagcgg ctgaaccaga cggtccgccc
     2941 accgctctta cgtccgttgc actccattcc gctcgtcatg ggagactggg tcggcaggga
     3001 cgaacccgtc aacgctgaca tcgtcgatcg atcgcagacc actgaatatc tcaaccgggt
     3061 gtatgaaagc cgcaagcagc ccggcgttcg cctccgcgtc tggatcaatt attctaacct
     3121 tggcaacaac ttgcgccaca cgcccgaaat ctgcctcccg tctggcggct ggaccaaaat
     3181 tgaatccatg acccgcgtga tcgacgtgcc acttgactcg gagcggtcga tggaagtcac
     3241 gaggttagca tatgcacgag gtgaactagt cgagcacgtg gggttctggt attacatttt
     3301 cggagaaggt aaactagaga attatgtgcg gcgccttccg attaccagcc ggagcagcca
     3361 cgggcaaaca acgcgcggtt cttcaatgac tgttgaggtt ttttatcccg gcgacaacga
     3421 tccggatggg gacgcgctgc gggaatttgc cgacgagctg gtaaagtcac tggagccgat
     3481 actaccactt gagcgagcca tctatcatat tccatgatgg gctgcgccgg attattcaac
     3541 ggaacaagat tgcatgaaac gcgctttgat caccgggatc accgggcagg acgggtcgta
     3601 tctcgccgaa atgctgctgg gcaagatgga ttacgaggtg cacggcttgg tccgtcgttc
     3661 cagtagtttg aatcgtcagc gaatcgacca ctttttcgcg cgtgatccgt cggttcacga
     3721 gcggtttcac ctccattacg ccgacctggc ggacgcctcg agccttgcca tggtcatgga
     3781 agaggtccga cccgacgagg tatataatct aggcgcccag agccacgttc gggtgtcgtt
     3841 cgatcagccg ctgtacactg ccgacgtggt cggcctgggc accttgcgtc tgctcgaggc
     3901 ggtccggcat ctgagcaagt cgaaatcggt caagttctat caggcgtcca gctcagaaat
     3961 gtacggctcg gcgccgccgc cccagggacc caacacgccc tttcacccgc gcagccccta
     4021 cgcatgcgcc aagctttatg gccactggca gaccatcaat taccgagaag catatggcct
     4081 ttttgcctgc tcgggaatct tgttcaacca cgaaagcccg cggcgcggtg agtcgttcgt
     4141 tacccgcaag gtcacgctgg gagccacgcg catcaaggaa ggcctccaaa agaaactcgt
     4201 catgggcaat ctcgactcca agcgcgattg gggcttcgcc ggcgactacg tccgcgcaat
     4261 gtggctgatg ctgcagcagg acaagcccga agactatgtg gtcgccaccg gcgaaacgca
     4321 ttcgatccac gagcttctcg atgttgcatt ctcgcacctg cagctcgact atcgcgactt
     4381 catcgagttt aacaccaaat acacgcgacc atcggaagtg gacgtcctcc tgggcgatgc
     4441 gaccaaagca cgtgagtctc tcggttggac ccccgaggtc gacttcaagg gtctggtgac
     4501 catgatggtc gagcatgacc tcgaactggc ccgccgcgag aaatttgcca agtcctaccg
     4561 cggcggctaa gaattgtgcg gcaacggccc ggatggccag cgtagatcac ggaagatcga
     4621 ggccgacatg gaaaaacgac gattcgccga ttcggtttta gttgcctgcc cggatgcgcg
     4681 accgccggcg taccaggccg ttgtcgggct ctgccgggcc aggctgcttc gatcgtttgt
     4741 gacgtcgacg tactacgacc gcgacggaca tctggcctcg ataatgcgca ggctcgctcc
     4801 ccatgcgctt gcccggctcg agggcgcgct actgcggcgg cacgagccgg aaattccggc
     4861 ggccttggtt cgcacgctcc ccagcttcga cgtggcgctg cgaatagagg ccaggatgga
     4921 ggcaacccat ccgcgcatca cacgggcgct cgcacgctgg cgcaccgacc agttcgacag
     4981 gcggttggcc cgcactgtcg ggcgatcgcg cctgggcgca gtcttactgt tcagcgacgt
     5041 cggctcggga gcaactctgc cactttgtcg ccggctcggc atcccgtcga tagtcagcat
     5101 ggtccacggc gacgttcgcg atgaagagcg cgtcatggag accgaatccg cgatcgcgcc
     5161 ggagttcatg ggaatgtatc tgggcggcgg atcgctcgat aggaagcttc tcgcctggct
     5221 ccatgagcgc cggctgcgcg acctggcagt ggccgaccgg atcctggttc cgtccgacca
     5281 catcgcccat cgtctgacgc agcagggcac cgcactcgac agattgcatg tcatccccta
     5341 cgcagccgac tgtcggcgtt ttggccccgg ctctgagaaa cgcgatccag acacctgcac
     5401 gtttctgttt gcggggggga tcagtcagcg caagggaatc aaatatctgc tcgaagcttg
     5461 gcaacggatc cgccgccctg gctggcggct ccaacttttg ggccccctgc cgagcaagcc
     5521 cgggccactc gggccttacc tcgaatcggt cgagccgctg ggacgcgttt cgcacgcgga
     5581 aatgcccgcg cggatgcggg aagccgacgt ctttgtgttt ccatcactct ttgagggttc
     5641 ggccgtcgtg acctacgagg ccctggctag cggcttgcct tgcgtggtca cggccgaagc
     5701 cggctcggtg gtacgcgacg gcctcgaggg atttgtcgtg gacgcgcgcg acgtcgccac
     5761 tctggccgcg cggatggaaa cgctcgcgcg cgatcgcgac ctgcgcgcct gcatggccgg
     5821 tgccgcccga gcccgcgcac tggccttcga ctggccgcgg taccaccgcg ccattgtcgc
     5881 cgtcgtcaac gaccttatcg gccaggaaaa cgacctcgcc ctcactcgac cagacggccg
     5941 gcatgcccga gggttcgtgc ccgcacgcgc gttcagccgc tcttaacccc gttgctgtat
     6001 ggaggcgcaa cgatgaaacg catcaaagaa ctcgactcga tccgcggcct ggccgcgctc
     6061 gtcatcgtat tctaccacct ctggtttcaa aatattggca tattgggcgc ggccgtcgat
     6121 ctcttctttg tcctgtccgg ctacctgatc acgacgatca tcctcgccaa tacgttcagc
     6181 gaacgtttct tgttcagctt ctacatgcga cgcggcttac gaatctggcc catttattac
     6241 ctgactctgg tcgccatggt cttgatttac gcctacctgc cgccgtgcgg caatttgaat
     6301 gacttgcctt attatctgac attcacgcag gaggtcgttc attacaggcc gtcgatggag
     6361 ccaagctttc cctcggcctt tcgccacacc tggagcctgg caatcgagga acagttttac
     6421 ctgatctggc cacccttggt ctggtggttg gggagcaaac ggctgccgat tgcctcgatg
     6481 ttgctggtgg tagtcgccgt cttcgcccgc gcgctagact tcagcgcctt ccttctgatc
     6541 acgcactgcg acggcctcgc gctaggggga cttttggccg gcattctggc cgcgcacgag
     6601 ggttcggtca cagggtcgcg gaagtatcgt ttgggaatgg gcattctggg tgtcgtgtcg
     6661 actgtcgcga ccatcgcggt catcgcgctt cccggatgct tgaactctca gtggcccggc
     6721 ctcgttcccg atgtgctggt tcgcgtgatc aaaccgctct gcctcaacct ggtgtttttc
     6781 gcggtcgtgg gcaatatcgt cagctacgcc ggcctaccgg gtctaggatg gctgcgcgac
     6841 agcaggctgg tctacttagg atcgatcagc tatggaatct atctctatca tcatataata
     6901 ttcgaagtat ttgcatatta cgctagcttt tacggatgga acgacagtgt tgttattgat
     6961 ttcggcaagg ttgctctctc catcgccctt gcggcactgt cgtggagatt cgtggagcaa
     7021 ccgatcctgg cgttcaagga tcgatttagc tatcgcgaag ccacccgggc ggtgctcgag
     7081 atcggcggcg cggttgacga cattcgcggt gtccaggcgg gttgagcttc catggacgat
     7141 ttatactctg tctatgcgac tggcgggttt atcctgctgt acttcctcat ccaggtggcg
     7201 agaggctctt tcgatccctt tgccccagtc tggctgttct ttgtcggctt tttgcaggtt
     7261 tatgtcctcc aggcgatatc gtaccacgag tgggcgattg agatccgggg gaaagacctg
     7321 gttgcggccg ccaactttcg tgcgttctgg gccctttcgt ggtttctgat tgtctatcaa
     7381 ttcgggcccg gcaggatgtt ctccgcactg ttgcccgatc cgccgcgggg ctggtcgact
     7441 gctgtcgtca gcgcgctcag cccgcccctg attctctggg gacttttctg cgcggggatg
     7501 gtcttgagaa gcggcgtgga cgcgcaggaa acggtttctg ccgaaggtac attactgctc
     7561 tcgttcccgt ttgtgatgat ggtcgcgggg gtcttgctga ttgtgactgg ccgctcgctg
     7621 gcgaatcccc gcccgcaatt cttgacggcg gggcttatgg tatcgggcgc ctatgtcttg
     7681 atctggatgt tcaacggcaa gcggtcgcat tcactgatcg gcgtgctggc aaccgtatgc
     7741 gcgttttata tcacacggct caagcgcccc tcatggcctg tcctcttggc taccggattc
     7801 acgggctcga tggtcgtggc gattgccatc ggctggcgcg gtaatctcga ttacgagcgg
     7861 tcgttcacgg gcttcatcag ttacctcggc gacttcaagg tcaacaaggt cctggaaagc
     7921 ttgaacgtca gtgaaggcga cgacgaggaa gaaagccgaa gtcatgagac cgccgagtat
     7981 ggtggcttcc tgctcatgat ggacacggtt ccccagaaat caggatacga ccacggccag
     8041 aactacattc gggtcttttc gacgttcatc cctcggctcc tctggcccag caagccgctc
     8101 tacggccgtg ccgagtgggt gagcgcctgg attgccggct cggaactgga acgcgacgaa
     8161 gaatttaccg gccccgcgat cggcatcctg ggcgcaaccc acttgaacgg aggcgcggcc
     8221 ggcacgttga tcgtactggc gtgtgtcgct ctctcgcttc gcactgccta cgaatatttc
     8281 cgcgctcatg cagatgtacc ctgggtccag ttttggtggt cgatcacctt ctacaattcg
     8341 tggttcatgg tcgtcggcga cgatcccatg acctggttct actacaactg gggtttcacg
     8401 acgttcccga tcgtcattgt tgtctggtta gcgaaccagg ccttggcccg aacgcagccc
     8461 ggacggctcg catcggccgc cgcgtaaatc gacgaccaaa gcggatctgg aagggccgat
     8521 cgtttgcgga aggaatccct gatgaagaca gcctggtctc ttgactcgat cacaaaatcg
     8581 ctcagatcct tcccactgac cgcgaatgag aagattgaca tctgtcgcgg cgtgttcgcg
     8641 ttcctcgtcg ttgccgccca cgcagtcgac ctctcgtggt caatccatcc cgacgctccg
     8701 gcccgatttt cgtggtggct gcacaggttt ttgctttatg tcgtcgccgc cggcgtgtac
     8761 tgggtcatcg gctttttcgt gatcagcggg tattgcattc agctttctgt atctcggagc
     8821 atttcagaaa actcgtttcc gctgacgcgc tatctggctg cccgcttcac gcgcattctc
     8881 ccgctctact atctggcgct ggccttcgcc gtggtcgtcg agtggctcat cgccggcgcg
     8941 cgacctccct actggtccaa cggaatcaat ggcagtgtac tcttcgccca gattttcatc
     9001 ttgcagaacc tctcccagac ctttggctcg ttcgcgccgt cttggagcat caccaacgag
     9061 atgttttact acctattcta tggcgcgttg gtctgtttcg cgctcaagcg tggcattcgt
     9121 gccgtcaagc tggggatgat cgtctgcctt gccgtcgctg tgccgatgga cttgctttat
     9181 ttcggctgga tccgttcgcg ctttgtcgcc aacgtgggtc tgctcttcgg tctgggaaca
     9241 ttctggtttt tgggcgcgtt cgtcgccgag tatcgcgaca ctctgagatg ctcgcatctg
     9301 gcaaggtcgg tgtcgcgctt ttggccgttg ctcttggcgg ctgccatcgc gatgtggttt
     9361 tctcagcgcg tccatatcct ggtggtgtac gtggtgctcg ctgtcgcctt caccgtgatg
     9421 ttgattcgca tcgtggcggc ggacaagggc tcgcccggtc gggacgaacg tcgtcgcccg
     9481 tcgcggctgg tggaaatgct cgggctcgcc agctatccga cctacttatt ccacggaccg
     9541 ctcctcttcc tcgtgggatc agcaatcttg cgctggaagt ttgtcagcga ttggcggctc
     9601 acctggttga ttctcgtgag cgtgggaata tccacgggga tcgcgctggg ttacctggcc
     9661 gagcgaccaa tcatgaaccg gcgggcagcc ttcttgagcc ggctccggtc gcagagaact
     9721 ccgcccgcgc ggggtgacgt caaggtcccg gtcctgagca tccagcaatg acacccgaag
     9781 cgggagcttg tcgaatgacc gttgccatct gtttcaccaa tttcggcccc tatcatcttg
     9841 cacggctgcg cgctctggcc gctcgtctgg gcacgacggg aatccggctg gttgcttatg
     9901 aggtcgcggg ccgcgagcaa gcgtacccct ggtcgccgga tcgaaacatc gaaccattcg
     9961 agtggatcac cctgttcccc gaccgggtct tggagacgat cgcgccggcc gattgtcggc
    10021 gcgcgatggc cgtggcactc gatcgcgatc agcccgacgt gctttgtctg gtcgggtacg
    10081 cgcgtccgga atcgatggcc gcggcgagtt gggccaagag gcacgggcgg ccggcgatct
    10141 tgctgtccga aagccaggcc atcgaccggc cacgcatctg gtggaaagag ctgatcaaga
    10201 tgcgaagaac acgccagttc gacgcggcct tggtcggtgg gccggcgcac cgcgactatc
    10261 tggtgcggct gggtatgccc cctgagcgca tcgccctggg atataacgcg gtcgacaatg
    10321 gctactttac caccgaggcc cagcgctggc gcaatagcgc gacgggtcgc gcgggcttgc
    10381 cggcagcgcc ctatttcctg acggtatgcc ggttcgcccc cgagaaaaac ctggtccgcc
    10441 tgatcagggc gtttgcgcgt taccgtgaag aatccaaccc ccaatcggcc tgggagctgg
    10501 tgctttgtgg cgatggtcca ggttccgccg aggtagaggg cgcaattgtc cgtagcgggt
    10561 gcgcggcggc gattcaccgc ccgggctttc tccagaccga cgcactcacg cgttggtacg
    10621 gccacgccga ggcatttgta ctaccgagtc tctcggagcc ctggggcctg gtcgtcaacg
    10681 aggctgccgc cagcggtttg ccgctcttgg tatccgcgcg cgccggctgc gcggccacgc
    10741 tcgtgccgga gccggaaggg acgaccggcg gccagtttga tccgctcgat gtagaagaac
    10801 tgacgacgaa gctggcgtgg gtcgcgagtc taactgcaga agaccggcgg gccatgggcc
    10861 ggcgcgcggc tcaggtcgtg tccgattggg gaccggaccg tttcgcccac ggggtcatcg
    10921 aggccatcga actcgccaga gccaaaacgc agtggccggc tcgtaccgct ttgaccgcga
    10981 cttccaggag ctaacgcgat gatcgcaacc cggcccaaca acaacgtcgc tctcgccatc
    11041 aacgcgcccc ctggtcgtcg cggctctccc cacgcggagg tgaatcccgc cacggggtgg
    11101 ctgcatctct gcaacggact cgaccccgtg cgtgatggtg gcatggtgcc gagtattctg
    11161 gggatgaccg aggccctgag tcgtctcaag ggggaagtca cgatcgtgac gccgacacga
    11221 tcgcggcttg atcaaacgca actcggcaac ggattgacac tgaaaggccc ggaaaccgac
    11281 ctggaagcgg cagtgggctc cgccgagatt gtccatatgc acgggctctg gcaggctcac
    11341 acccgccgcg gcgccagggc agcccgcgcc gcgcgtgtcc cgtatctgat cgcggcgcac
    11401 ggcatggccg agccctgggc cctacggcac aaacgctgga aaaagcgcgt ctacatggcg
    11461 ctcgtcgagg ccaagaatct gcggcaggca tcgtgcctgc acgcgctggc tcgtcccgaa
    11521 atcgcccacc ttcgaagcat tgctccccgg acgcccatct gcttcgttcc caatggtgtc
    11581 gatcttgcgc cttttgaaaa catgccgcct cggtctgtcc tcgaggccca ataccctgag
    11641 ctcaagggaa agttcgtgct gctctttttt ggccgagttc atgtcaagaa gggcctcgac
    11701 ttgctggctg aagcgctcta ccgaacgatt ccagaccacc gcgcgctcca cctcgtcgtt
    11761 gccggcactg acgacggcgc ctggtctccg ttccgagatc ggatcgcaag cctgggcctg
    11821 accgactcaa tgacgtatct cgggcatgtc gccgccgagc gagcgcggca ggtgtgggga
    11881 gcggccgacg cctttgttct ccccagctac agcgaaggat tcagtatggc cgtcctcgag
    11941 gcgctggcat gctccctgcc gtgcctggtc acgaccgctt gccatttccc cgagttggcg
    12001 gcggcgggcg gtggcatcga ggtcgccgcc accgccgagt ccgtcaccca gggcctgcgc
    12061 gacttagtcg agcggactcc cgccgagcgc gcacgactcg gccaggtcgg ccgtcggctt
    12121 gtcgaagaaa actacacatg ggaccgtcag gcggaacggc tcgcgtcggt ctatgcatgg
    12181 ctcaaagggg gagggccgcc ccctgagccg gtcgtgcctt gaattccccg cacgttttgc
    12241 cccaaagggg cacagacata aaagcccagg gcaacgccct ggggaggcaa cgccttgtga
    12301 ggtccgcgac ctatgggtaa gacaccggtc agtgtgatca tcccggtcag gaacgaggct
    12361 gaaaacctcg agcgctgcct gccagcgctg gcgtgggccg acgaggtctt cgttgtcgac
    12421 agccagagca gcgatcagac cgcggaagtc gccgagcgat ctggcgcgtc ggtggttcag
    12481 ttccatttca acggggccta ccccaagaag aaaaactggg cacttgataa cctgccattc
    12541 cgcaatgagt gggtcttgat cgtcgatgcc gacgaagtgg tcatacccga gctcgcaacg
    12601 gaaatcgccg ggcggatcgc ccgcgatgaa gccgacggat tttatctgaa ctcacagtac
    12661 tacttcctcg gccggcggat ccggcactgt ggttacgcgt cgtgctggaa tctgcgtttg
    12721 ttcaagcatc ggctcggccg ctacgaaaga atgcccgacg tcaccggcgg ccgcacggga
    12781 gataatgaag cgcacgagca tgtcgagctc gagggccgcg tgttgcggct caaccacgag
    12841 ctggaacacc acgcctaccc aacagtggcc atctgggtcg aaaagcacaa tcgctacgct
    12901 atatgggagg ccgcacttgc cgagcgcctg atacatgagc cgattcccaa ctcgatcggc
    12961 agcgtccagc gctacaagcg acgactgaaa aagatcgcct ggcgactgcc gctacgtcct
    13021 ttggttcgat tcctttacgc gtatgtggcg cgcctcggct tcctggacgg caaaccagga
    13081 ctttatttct gtgggcttct ggccttttac gactttctgg ccatggcaaa ccgctatgag
    13141 cgacaactcg cggaaacagg tgcgactcca ggcgtggcag ccgggttccc gggctcgcgt
    13201 cgccaggcgc ttcttgattc gaggttgcca aattcccgaa tccgagtatt ttgagggccg
    13261 ccgctcccga gatcgtgacg cccagaacgg cgcttgtcat cgagtcgcga ccgcgagcca
    13321 acgaccgaca aatggattca tcatcaggga ggatcatccg tggtgcgcgg aatcattgag
    13381 aatttcaccc gtgattgggt gatccgacgg cggctatcgg ccgacttttc gagtgcaccg
    13441 attcacgtca cgccatcggc gggacttcgc tacctcttcc ggccgatgaa ttcggtcgat
    13501 ccttcattgc tcgaactcgt gaaggagttt atagctccgg gcgccgtcgt ctgggatatc
    13561 ggagcaaaca tcggcctttt ctcgttcgcg gccgcgagca tcgcgggacc tgaagggctt
    13621 gtggtcgcgc tcgagcccga tgcctggcta gtgcagcttt tgcggcgtag tgcgctcgaa
    13681 caaccggcgg gctcggcctc ggtgcaagtc gtacccgccg cggtggcttc cgaggtctcg
    13741 ctgcgtacgc tctgcctcgc caaccggtcg cgggccgcaa attacctggc cgaattcggc
    13801 acgacgcaga ccggcggttc gcgcgagcaa cagtcggtgg ttgcggtgac tctcgactgg
    13861 ctcatggact cattgccggc tccctcggtc ctcaagatcg acgtcgaagg agccgagctc
    13921 gaggtgctca aaggtgcgca tcggctgttc gagacggtcc ggccggtcgt cttctgcgag
    13981 gtgattccgg ccagcgaacc gggtgtcacc gattttttca acgagcacaa ctaccagatc
    14041 ttcaacggcg agctcccctc cggcgagcga caaccgctgt cggcctcacc ttggtgcaca
    14101 gtcgcgatcc ccgcctgaaa ggcggcgagt agtactgctg gatcaccctt agaccgaggt
    14161 ccccgaagaa gtcgacgtcg aagtttgcgc ggccgcagtg ccggatcccg ccccggcgct
    14221 gcctgaattc gccgaggacg aggtcgtggt cgacgtctgg gcgctaggcg ccggagatcc
    14281 cgcgttactg gaattcgccg acgatgaggt cgaagtctgc gcggatgaat tgcctgagcc
    14341 ggaatcgccg gcggcggtgt tggcgtttgt agacgttgaa gtcgaagcct gtgctcccgg
    14401 cgacgaaggt gtgggcgtgg tcacggcggt cgggctcgtc gcggtattgc ccgtcgttgt
    14461 cgcggaggtc gttccagttg tcgtctgcgc actcgaaccg cccgatcccg cctgggtgcc
    14521 cgtcgaggtc gtcgtaccgg tttgagcgct tgagctgcct ccagccgcct gggttcctgt
    14581 cgaattcgtc gtgccggttt gagcgctcga gctgactcca gccgcctggg tt
//
LOCUS       DATAQL010001405         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3045963, whole genome shotgun sequence.
ACCESSION   DATAQL010001405 DATAQL010000000
VERSION     DATAQL010001405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045963"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VHS97_24200"
     CDS             complement(<1..1120)
                     /locus_tag="VHS97_24200"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX3451378.1"
                     /translation="MFEDDVLERADLGAQEAGTIQSVPALPHEANDRPSNETARVRDL
                     PQQKTPAAMRSAPPDEFKEASPPAIGKSHRNGWLRRRPVVSAIAALLFALALGGGYLY
                     LDHARRFQSTDDAFIAARQFSIAPKVSGYLTAVPVTDNQHVKAGDVIARIDDRDYRTA
                     LAQAEAQVAAAHASIENIDAQLDVQQAQIAANRAQVDQAQAALVFAEQQAARYEHLGQ
                     TGYGTVQNEQQYTSQLHQQQAALASAQATLKLAQRQVEALKAQRNGAVASLGQAEAQR
                     DQARLNLSYTTVMAAQPGRVVSLTVAVGQFVQPGTNLTMFVPDEIWVTANFKEIQLDH
                     MRPGDPVTLAIDAYPERRIHGHVESVQPGSGTAFSLLPA"
     gene            complement(1113..1397)
                     /locus_tag="VHS97_24205"
     CDS             complement(1113..1397)
                     /locus_tag="VHS97_24205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451379.1"
                     /translation="MSKLLAIAATLKAILIAASFALARGDMLSVILISALFNLAHVGA
                     RNSGERTRRDSTVASRGHDQWRLDPLNPTQLNESRAPRLALLTWRIDDHV"
     gene            1555..>2112
                     /locus_tag="VHS97_24210"
     CDS             1555..>2112
                     /locus_tag="VHS97_24210"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3451380.1"
                     /translation="MDLLGTFKVLVRVTETGSFSAVARERDLTQAAVAKQISQLEEHF
                     GVRLFHRTTRKLSLTDDGRMLLDLARPVLDGVEGIEASLGRQSASPVGLVRVGVTGTA
                     SRLLAQRLPTLLAGHPGLKVELVISDRFGDMIEDRLDLAMRVGEVTDASLVVRRSGAA
                     RFVVVAAPSYIERKGSPATPADLAGH"
BASE COUNT          373 a          636 c          667 g          436 t
ORIGIN      
        1 gtgccggcaa cagcgaaaac gcggtgccag atccaggctg gacgctctcg acatgaccgt
       61 ggatcctgcg ttccggatag gcgtcgatcg cgagcgtcac cggatcgccg ggccgcatat
      121 ggtcgagttg aatctccttg aagttcgccg tcacccagat ttcatccggc acgaacatgg
      181 tcaggttggt gcccggctgg acgaattggc cgactgcgac ggtcaggctg acgacacgcc
      241 ccggctgggc cgccatcact gtcgtgtacg acagattgag tcgcgcctga tcacgctgtg
      301 cttcagcctg cccaaggctt gcaaccgcgc cattgcgttg agccttcagc gcctcgactt
      361 gccgctgcgc cagcttaagg gtcgcctgtg cgctcgcaag cgcagcctgc tgctggtgga
      421 gctgcgaggt atattgttgt tcgttctgaa cggtgccata gccggtttgc ccgagatgct
      481 cgtaccgtgc cgcctgctgc tcggcgaaaa ccaacgcggc ttgcgcctga tccacctgtg
      541 cccgattggc ggcgatctgc gcctgctgaa catcaagttg cgcatcgata ttctcgatgc
      601 tggcatgcgc ggccgcaact tgtgcctctg cctgcgcgag cgccgtgcga taatcgcggt
      661 cgtcgatgcg ggcgatcaca tcgccggcct tgacatgctg gttgtcggtg acggggacgg
      721 cggttagata gccagagact ttcggtgcga tcgaaaactg ccgcgccgcg atgaaggcgt
      781 cgtcggtgga ttgaaaacgc ctggcgtggt ccagataaag atagccgcca cccagtgcta
      841 aagcgaaaag aagcgcagcg atcgccgaga caacagggcg acgccgtagc cacccgtttc
      901 ggtgcgattt gccgatcgct ggcggcgagg cttctttgaa ctcatccggt ggggccgacc
      961 gcatggcggc aggagttttt tgttgcggga gatcccgcac cctggccgtt tcgttcgacg
     1021 gccggtcgtt cgcctcgtgc ggcaaggccg ggacggattg gatagttcca gcctcttgag
     1081 ccccgagatc ggccctttcc agaacatcgt cttcaaacat gatcgtcgat cctccatgtc
     1141 aggagagcca ggcgcggcgc tcgcgattcg ttcagttgag ttgggttgag tggatcaagc
     1201 cgccactgat cgtggccgcg cgaagctacc gtgctgtcgc gccgcgtcct ttcaccggaa
     1261 ttcctcgccc ctacatgggc cagattgaac aaggccgaga tcaggatgac cgagagcatg
     1321 tctccgcgcg ccaaagcgaa gctcgccgcg atgaggatgg ccttcagagt ggccgcgatt
     1381 gcgagaagtt ttgacatcgg gcacctcgct ttcttctccg gactgcctgg caccagcaaa
     1441 gggcgaccgc ctcacgtgta tcgctggcct gctacgctgc tgacatagcc ccttgcatca
     1501 gtgggattag cccgcatctc ttcccatgag ttttgacttt ctgtggggaa atcgatggac
     1561 cttctgggca cgttcaaagt cctcgtgcgg gtcacggaga ccggttcctt ttcggctgtg
     1621 gcccgtgagc gcgatctaac ccaagctgcg gtcgccaagc agatttctca gcttgaagag
     1681 catttcggcg tgcgcctatt ccaccgcacg acaagaaagc tcagcctgac ggacgatgga
     1741 cggatgctgc tggacttggc gcggccggta ctggacggcg tcgagggaat cgaagcgtct
     1801 cttggaagac agagcgcctc gccggtgggg ctggtgcgcg tgggtgtcac aggaacagcc
     1861 agccgtttgc tcgcccaacg tctgccgacc ctgctcgccg gtcatccagg gctaaaggtc
     1921 gaactcgtga tcagcgatcg gttcggcgac atgatcgaag accggcttga cctcgctatg
     1981 cgtgttggcg aggtcacgga tgcttcccta gtggtgcgcc gatcgggagc ggctcggttt
     2041 gttgttgtgg cagcgccaag ctacatcgag cgaaaaggca gccccgctac acccgccgat
     2101 cttgccggtc ac
//
LOCUS       DATAQL010001406         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3501288, whole genome shotgun sequence.
ACCESSION   DATAQL010001406 DATAQL010000000
VERSION     DATAQL010001406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501288"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..608)
                     /locus_tag="VHS97_24215"
     CDS             complement(93..608)
                     /locus_tag="VHS97_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451381.1"
                     /translation="MYHTNRAGRGVTLAVLLTLCPPALEAVAGQDTAVPARNTSVFDV
                     PNDAELFAAYQSDHSNQKVQSWGQYRGWVQTFYKGNLMSDGWTKFSEVTVSKVKSAEP
                     RQAVIAHINELGRIISLEWAKDSSVRKITTADLRSWNEAMATARQSDYGSGQQIMDAL
                     KAVRRMAENRR"
     gene            854..1834
                     /locus_tag="VHS97_24220"
     CDS             854..1834
                     /locus_tag="VHS97_24220"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEX3451382.1"
                     /translation="MMWFYCLMVALGTAEKDAAEATDWMKPATWQGNFWGPAATRARS
                     TGALPPLPTNPVMTHWATWGKNLLRDGDIVFRLGDAKALGGTFPLSQFIATATGSRFS
                     HTGIVSVESGSPVVYDCSSAGVQRQPFEVWMLDCVGAVGVKRLKLEYRNHVPAILGYC
                     RAKFEQQVPFDFEFRPDDAAFYCVELTEKAFRSQGLKLSEPVRIGDWEHLGSYPLTAL
                     ATPLVTKLFLTEPISLEQPVYVPGNDHEGVWSSSLLETVYSAEPPASVLAAHADLARV
                     NLRGDFDLIRFAIGAVRHVNQPSVTSHDVRGRQAVSAPDSRTATSVATTN"
     gene            complement(2345..2417)
                     /locus_tag="VHS97_24225"
     tRNA            complement(2345..2417)
                     /locus_tag="VHS97_24225"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2382..2384),aa:Val,seq:cac)
     gene            2623..>3923
                     /gene="xylA"
                     /locus_tag="VHS97_24230"
     CDS             2623..>3923
                     /gene="xylA"
                     /locus_tag="VHS97_24230"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX3451383.1"
                     /translation="MAEFFPGVPKIQFGGPKSRNPLDFKHYNPDEVVDGKTMKEHLRF
                     SVVYWHTFCNALSDPFGVGTALRPWDDGSNSVANAQRRAKVAFELFDKLGAPFYAFHD
                     RDVAPEGTNLKESHANLDEVVKVLKDEQSRTGVKLLWGTANLFTNPRYMHGAATSPNF
                     DVFAFAGAQVKKAMEVTHALGGTGYTFWGGREGYSTLLNTDMKRELDHLGRFLHMAVN
                     YKKQIGFQGTFYIEPKPKEPTKHQYDSDAAACLNFLRTYELLPHFKLNLETNHATLAG
                     HEMMHELEVAIGAGALGSIDANTGDYLVGWDTDQFPTSIYLTTQCMLPILGMGGLTTG
                     GINFDAKVRRESFEPIDLFHAHIGGMDAFARGFKIARAIIADGRLAAFVKERYRSWDC
                     ELGRRVEGGNASFGDLESYILPKGDAAKNSSGRQEMLENLI"
BASE COUNT          805 a         1186 c         1092 g          840 t
ORIGIN      
        1 ccattaaccg gctcctacca acacgcatcg gcgcgatcca tttacatcga cagaatgcct
       61 tagcgacgga cgtcgtcacc ctggtcatcg cgtcagcgcc ggttctcggc cattcttcgg
      121 accgctttca gcgcgtccat aatctgttgt ccgctgccat agtcgctctg tcgcgcggtg
      181 gccatcgctt cgttccagct tcttaggtcg gccgtggtga tctttcgcac gctcgaatcc
      241 ttggcccatt cgagactgat gattcttccc agctcgttga tatgggcgat gacggcttgc
      301 cgtggttctg cggatttcac tttgctcacg gtcacttcac taaacttggt ccagccgtcc
      361 gacatcagat tacctttgta gaaggtctgg acccagcccc ggtattgccc ccaactctgc
      421 accttttggt tcgagtggtc gctctgatag gctgcaaaca actcggcatc gttgggcaca
      481 tcaaaaacgg acgtattccg ggcgggcacg gccgtgtctt ggccagcgac ggcttctagg
      541 gcgggcgggc acaacgtgag taacaccgca agagtcaccc cacgtccagc acgattggtg
      601 tgatacatcg ttctctttcc ctctacgttt cttggttcgt ctcgggtgta tctcctcgtc
      661 tcgttatcgc cacccgcgat gcgatcggac aaatttccgt cggcccagtc cttttcttga
      721 tgattttcta ttgctggatg ccttacccga gaaaatccat ggtccgatat ccgctcggct
      781 cgcggatggt gataacccca ttgagagggc cgggcgtcgg ctcgattcga agtgtcgaga
      841 ggggagtttc gccatgatgt ggttctactg cttgatggtc gcactcggga ctgctgagaa
      901 agatgcggcg gaagcaacag attggatgaa gccggcgacg tggcagggaa acttttgggg
      961 cccggcagcg acccgtgccc ggtcgacggg agcactgcca ccgcttccga ctaacccggt
     1021 gatgacccat tgggccactt ggggcaagaa cttgcttcga gacggagaca tcgtctttcg
     1081 gctcggcgac gcgaaagccc tgggcggcac gtttccgctc agtcagttca tcgccaccgc
     1141 caccggcagt cgcttctctc acacggggat cgtatcggtg gagagcggct caccggtggt
     1201 ttacgattgc tcgtcggccg gcgttcagcg ccagccgttc gaagtttgga tgctcgactg
     1261 cgtcggtgcg gtgggcgtca agcggctcaa gcttgagtac cggaatcacg tcccagccat
     1321 cctcggctat tgtcgcgcaa agttcgagca gcaggttccg ttcgactttg aattccgccc
     1381 ggacgatgcc gcgttttatt gtgtcgagct cacggaaaaa gcgttccgat ctcaggggct
     1441 gaagctctct gaacccgtgc ggatcggcga ttgggagcat ctgggcagct atccgctgac
     1501 cgctcttgcg acaccactcg tcactaaact cttcttgacc gagccgatca gtttggagca
     1561 gcccgtttac gtgccgggaa acgatcacga gggtgtgtgg tcttcatcct tgctggagac
     1621 cgtttacagt gcggagccgc cggccagcgt actggcggcg cacgcagacc tcgcccgcgt
     1681 caacctgcgg ggagactttg atttgatccg gttcgccatc ggagcagttc gccacgtgaa
     1741 tcagccgtcg gtgacttcgc atgacgttcg cggaaggcaa gcggtttctg ctccggattc
     1801 gcggacggcg acgtctgtcg cgacgaccaa ttgagcatcc tcccagtaag tttcatcatg
     1861 caagtcttgc cccgaacgat tggacgcgcc aatgcacacc tgtaatcgtg cccgttcgcg
     1921 caacgttcaa tccatcgggc gtcgcttata cgtttgccga cactcggttc agaggtccgc
     1981 cgcacaggtt agccctccaa agctaagcgc gaagtgcgag aacgaaaacc gagcacggag
     2041 cagcaatcgc cgtcagtcca atgggcggcg aaggattcga acctgtgact tccaccgtgt
     2101 gagaacggcc cgcattggcg aaatccctgc agacgcctcg cgagtcaatt cacgatctag
     2161 tgaaagaggc aaaacccgac acccaaggct accggaccgt agcgccaact ccgggcaaga
     2221 acagggttgg gagtcgattc ccgggaaccg gttagaggtc caccgttaag aattgcactg
     2281 tcaaaatcat cgccgggagc aaaacgaaaa ctgacgatct ggcggcgttt cgccgtaagt
     2341 ctaatgggcg gcgcgggatt cgaacctgcg acttccaccg tgtgaggatg gcactctaac
     2401 cgctgagtta gccgcccaat cgtgactgac cggactcgat ctcaatccag tcacttcaga
     2461 atcaaaaata gcgactcaca ctgccgtcgg caagagtcat gacacccggc tcaatcccga
     2521 ggtcgacact gaggggttgc ccaaaaccta gcgccgtctt agcattatcg cgtgggtgtc
     2581 tgtaccgatt gtcaggttgg tgaactgtgt gagtcccctg cgatggccga gtttttcccc
     2641 ggcgtgccca agatccagtt cggtggccct aagtcccgca atccgctgga tttcaaacat
     2701 tataatcccg atgaggtcgt cgatggaaaa acgatgaagg agcacctccg cttctcggtc
     2761 gtttactggc ataccttctg taacgcgctt tccgatccat tcggagtcgg tacggctttg
     2821 cgcccctggg acgatggcag caactccgtt gccaacgccc agcgccgggc caaagtcgcc
     2881 ttcgagctct tcgacaaact cggcgccccc ttttacgctt tccacgaccg cgatgtcgcc
     2941 cccgagggaa ccaacctcaa ggagagccac gccaacctcg atgaagtggt caaggtcttg
     3001 aaagacgaac aatctcggac cggcgtcaag ctgctctggg gaacggccaa cctctttacc
     3061 aaccctcgat acatgcacgg cgcagccacg agccccaatt tcgacgtatt cgcctttgcg
     3121 ggagcacagg tcaaaaaagc catggaggtc acccatgcgc tcgggggcac cggctacact
     3181 ttttggggtg gtcgcgaggg ctattcgacc ttgctcaaca ccgacatgaa gcgcgagctc
     3241 gaccacctcg gccgctttct gcacatggcc gtcaactaca aaaaacaaat cggcttccag
     3301 gggacttttt atatcgagcc caagcccaag gaacccacaa agcaccagta tgattccgac
     3361 gccgcggcat gcctcaactt tttgaggaca tacgagcttc tcccccactt caaactcaat
     3421 ctcgaaacca accatgcgac ccttgctggg catgagatga tgcacgagct cgaagtcgcc
     3481 atcggcgcag gcgcactggg gtcgatcgac gccaacaccg gtgattactt ggtcggatgg
     3541 gataccgatc aattccctac cagtatctat ctcacaaccc agtgcatgct tcccatactc
     3601 ggtatgggag ggctcaccac cggcggtatc aatttcgatg ccaaggtccg tcgcgagagc
     3661 ttcgagccaa tcgacctatt tcatgcgcat atcgggggca tggatgcctt tgcccgtggg
     3721 ttcaagatcg cgcgcgcaat cattgccgac ggccggctcg cggcgttcgt caaggaacgc
     3781 taccggagct gggactgcga gctgggccgg cgcgtcgaag gcggcaacgc aagctttggc
     3841 gatctcgaat cctatatcct ccccaaggga gacgccgcca agaattccag cggccggcag
     3901 gagatgctcg agaatcttat taa
//
LOCUS       DATAQL010001407        13641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3808889, whole genome shotgun sequence.
ACCESSION   DATAQL010001407 DATAQL010000000
VERSION     DATAQL010001407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 13641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13641
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808889"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..291)
                     /locus_tag="VHS97_24235"
     CDS             complement(73..291)
                     /locus_tag="VHS97_24235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451384.1"
                     /translation="MYALKRIAAYAIDMAIFWLPMTLLINLGEAPLVGRMPARFHMFG
                     ALGSWGFSILGPVLVNGTLTGLTGRSPG"
     gene            complement(594..1202)
                     /gene="hisH"
                     /locus_tag="VHS97_24240"
     CDS             complement(594..1202)
                     /gene="hisH"
                     /locus_tag="VHS97_24240"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX3451385.1"
                     /translation="MIVIIDYGMGNLRSVQKAIEAVGSAAEITSDPHRVAQASKVILP
                     GVGAFADAMAELRRTGLGEAFREAVHKGKPCLGVCLGLQLLFDTSSEDGEHVGLGLLP
                     GRVVRFPARPGLKVPHMGWNTLRIKKPAPLLAGLPAEPSVYFVHSYHAVAAVEDDIAA
                     ESDYPEPFAAVVWRDNLTACQFHPEKSQAMGPAMYANFVATG"
     gene            complement(1303..2070)
                     /locus_tag="VHS97_24245"
     CDS             complement(1303..2070)
                     /locus_tag="VHS97_24245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3451386.1"
                     /translation="MTGENTQRFDDAQELKELGHLAAAIGHNLINAFSAIVSNAELVR
                     SRSIDTSDPSELDSLASGIIETSLDAAQIARKLIDWTRRTTAVDVDTPGHGTSDIDLN
                     KLLTETVELEKVLAPPQVSWAVDLAPIPPIPGLAAHLRTMFGHLLRNACESLPGGAGT
                     VAVSTYIDPRNWLVVAIRDSGCGMSAEVLRHATEPFFTTKPDQSGVGLTIAQGIWRRH
                     RGALSIDSRPGQGTTIRLSIGPLSKAGPDNPSPPRTS"
     gene            complement(2168..3430)
                     /locus_tag="VHS97_24250"
     CDS             complement(2168..3430)
                     /locus_tag="VHS97_24250"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEX3451387.1"
                     /translation="MLFLPRKTCSLYRGALVAAALSITHFADGGASALAATSSGAASD
                     PVFKALLVDGSTVSGRIVSLGPGAIKLASAEGPARELPLGRLIKLTREYSVAFPSLDR
                     SHVIFPGGDCLMRAVVGSSAETTLDVRSDALGKMAIPLESMLGLYLAGQRSDSELDPI
                     MDRVRSEPRTTEVVWLANGDRQAGGFLGIDENKITMQVGGKPAMVDRAGVVALGFDPA
                     LVVYPLPNSDFLELTLKDGSRLGVTEARLDDSTVLATTRFGQALKVSLNELVRVHVRS
                     KSVVYLSEREPIRVSYVPYFGPTREIRVDRTVDGHYFQLAGQTFDRGIGMQSKTLVAY
                     RLQPGDRRFQALVGVDERAGPLGSVVFRVLVDSNERFQTPPLTDHDSPKTIDVDVAGG
                     KFVILATEFGDRGDVRDLADWVEARIIR"
     gene            complement(3448..4563)
                     /locus_tag="VHS97_24255"
     CDS             complement(3448..4563)
                     /locus_tag="VHS97_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX3451388.1"
                     /translation="MGADRLGLFSDCASARFWVGLALTLALGATARAQATDQTKAAQG
                     FGESRRGDVPDGTLEMMTPETDQAIKTGLLWLARNQNADGSYGTQSYRGNIAVTSLAG
                     LAFMASGSSPGRGPYGSQIDRALAYVMRNTSPSGFIAVLATSTHGPMYSHGFGTLFLA
                     EAYGMTHRPEIREKLQKAVRLIIDSQNIEGGWRYQPVRRDADLSVTICQINALRAARN
                     AGLFVPKEIVDACIRYVKQAQNPDGGFRYMLQGGATSEFPRSAAGIVALQSAGEYDSK
                     EVRDGIAYLRHYSQGLRVGRRNNHYFYGQYYAAQAMWLEGKEAWAEWYPAIRKELVDK
                     QSASGFWTDSGVCNEYGTAMALIILQIPNDYLPIFQR"
     gene            complement(4597..5712)
                     /locus_tag="VHS97_24260"
     CDS             complement(4597..5712)
                     /locus_tag="VHS97_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX3451389.1"
                     /translation="MAALRAVRACAFARSSALCWFVILIMSGGSALAQEAARDQGKSV
                     EGFGEARRGDVPAGTLEMMTPETDNAIKSGLAWLARTQSADGSFGTGTYRGNIAVTSV
                     AGLAFMASGSSPGRGPYGPQIDKALVFVMDNTSPAGFIAVVAASTHGPMYSHGFGTLF
                     LAEAYGMTRRPEIREKLQKAVRLIVDTQNVEGGWRYQPIRRDADLSVTICQINALRAA
                     RNAGLFVPKETVDACIRYVQKSQNPDGGFRYMLQGGASAFPRSAAGVVALQSAGEYDT
                     KEVKEGITYLRQYMREIKLGNRYSHYFYGHYYAAQAMWLRGGDDWAEWYPAIRNELLH
                     RQEVSGFWSDSICNEYGTAMALIILQIPNNYLPIFQR"
     gene            complement(5717..6712)
                     /locus_tag="VHS97_24265"
     CDS             complement(5717..6712)
                     /locus_tag="VHS97_24265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451390.1"
                     /translation="MMLKSAKFILKFAILAAIMGGDRAWAQDTAKSKDDALDSLMKEL
                     RESEKAPAQPAKPGTAETQKPTRPQDKSKVEPRAKATGDKDGQAPAAKSDKPSAGKSP
                     DAAKLAPKDQAIDDLLGKLGESKDDPTAAPEPRNRAPGQGPTEPSRGQKPGGDKLGAK
                     DKDLDERLEELTGRKKKRPSSEQERTGPIGDMIKEMREVEQRLGKPDPSEDTQKKQKQ
                     IVKRIETLIEQVKQQGGSAGSMAMRRMQQQGRQPGQQEGNQPGAQARGAPAMKPAKPT
                     SQHSTAGGKDVWGHLPPELRKIMDNSFKETELDSKKELISRYFLSVDKGKLVREE"
     gene            complement(6716..10576)
                     /locus_tag="VHS97_24270"
     CDS             complement(6716..10576)
                     /locus_tag="VHS97_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HEX3451391.1"
                     /translation="MTPRQLESRIRALRGSLRRLLALHGLSWVLGILVPLVVVAGFAD
                     WAFQLDSVIRATLLAALAGGLIYLGYRRVLRPLFVKFADLDIAMRIEERWPGFNDRLA
                     STIQFVRLDAQDKRFGSQALREATVRQTVEEASKIDFREVIEPKPVVRALGVAASALA
                     LGALLLLIAPVTSRIALKRLFVPFGSTSWPRQTHLILDEGLTTLKVARGDSFTLAVKV
                     RPGDKVPESAEATYLFADGESAGEPMRSIEGGEFRGRIDSVNQPFRFSVAAGDDSTSI
                     RDVEVKVVPPPTLKSLAIKLIPPTYTGIKEQALAPGLTQLRALEGTQLELDGIANKPL
                     AHAELRQGENEAGTALTFDTSATRFKTTLPVQGTFGFWFDLKDTEGFRNREAVRYDVR
                     GFRDEAPRVVIEEPRADRDVPADSTIPVRITLDDDFGLHSARLIYTLATGDSEPHAEV
                     AIPLWSAQEQEPKGAAAGFTKHQEISHRWELAPLKLQVGTVITFYGNARDFDTIKGPN
                     VGKSREIRLRIVSKEDAVRQFDDARRELRDELSRVLNMQKQAITPVDNAVRTLSQTDR
                     LAARERDDLKNGTMIQRQVTSRINNRDEGVGARISHVLDDLKNFKIDNADAQKQMEDM
                     LARLGMIRDQHLGPAEQQLARATKTLEDEAAATKDAQPSPAANPADNKPAAADQAQPD
                     SKDQTQKGASPSPAAKATEAKPATAKQLESAAETGNERGDRSPGKNEQGDASKGNERS
                     GEAAKEASKPETPSKTSPTQPGKESTQLALAESNKNQKAIANELQKMLDGLSEFETYR
                     GVVKDAQELLKQHEQTMKQTDEAADKPDTRGKPLDALNQEQKADLGNLASRQSQVGKG
                     LQNLQERMAEMARRMDESDPMAASAMREAAEKSRQKGTAGKLAEAADQLEKNQMGQAQ
                     ARQEQAREELRDLVDNIQNRRERELARLVKELKNAEAELANTRARQAQNLKKTREAQR
                     NPDAKQRADQLKRLAKEQAEIQKDLKRQLQKLAKLSADRAAQKGQTASGKMDRAQGDL
                     DDDQGEQAGKEQEEALADLDDAQEELEQTRKDAEEQLAMEQLVRMGDRLKGLAERQAK
                     VVSETSSFEAMRQKNDGKLSIAQRTGVRGLGQVQSGLKEETAELIENLDGAPVFALTL
                     RRAKESMENAAKRLAELKTDGLTEQAAKAASDRFKQLLESLKSDSAGGGQGGGGGGGG
                     GGGGAGGNGGGDGIPATAQLKMLKSLQQEINERTEALDEVQRRNQKLTSDQDAELRRL
                     GEEQGVLADLVRDMTRPKRDDGEE"
     gene            complement(10655..13126)
                     /locus_tag="VHS97_24275"
     CDS             complement(10655..13126)
                     /locus_tag="VHS97_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3451392.1"
                     /translation="MTDWLRGLKVTDWLLQGLADRLGVAPPQAGEATNPRIRFEQPWD
                     QTLLVFTAVLSVALIVWLYRHEGKASTATKIFLAGLRIAIVFLAMFMLSEAVLSVERT
                     GLPYVTILIDDSDSQRIADQYEKPEVQKALDELAALSGPDGVGATATTTNDKETTRLD
                     IAKGLILQDQAKLLSELEKQHKVRLYLVSSATRNLADVDRPRDLDEAVKRVRGILPQG
                     SQSRLGDGVRNVLTELRGSPPSAIVLFTDGQTTEGEPLSKAAALAARKGVPIFAVGLG
                     SAEPARDIELTELLVDDVVFVDDTVRFQAKLLARGFQGEKVVVRLKELEPGSRDPKTA
                     RELEKVEVDAPPNGEPKRVELVHHPKTTGERTYILEVDKKPRELQIENNLIERMVTVR
                     KEKLKVLFVDSEPRYEFRYLKNYLERDETIDLNIVLLSSDPAYSQQDRIALPTFPAAK
                     DDLFAYDVVLFGDADTSFLSQSQMQNLFEFVNEKGGGVLFVAGELFNPLSYRGSPLEV
                     LLPVELADARNPTAVGTGITSFRPELTLEGRSSPIFRFGDSEAASIEIWQKLPELFWY
                     LEAPRKKPAALVLAEHPTAIGSDGKLPLVLYHFVGAGKAMFHAFDDTWRWRFRVGDRY
                     FGRFWVQTIRFMARSKLVGQRQAEVQTDRKRYQRGQPIQFRVRFPNPGLAPTTGDIAV
                     QLERRGQGPRKLALQLVPGTRNVFEGALAQTPEGEYDVRLLPPPVLEGPIPSTSFRVE
                     APVNEFERIEMNQPELIRVAESTGGKFYTPLTAVTLLKDLPKPSKVPLDTDPPIPLWN
                     TWPILTLFLLLITTEWVFRKRKQMV"
     gene            complement(13139..>13641)
                     /locus_tag="VHS97_24280"
     CDS             complement(13139..>13641)
                     /locus_tag="VHS97_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451393.1"
                     /translation="PVMQEIVLRAAAGRMSERNIRVGQPFDQSFPSAGAAAPVTVVPP
                     RGAPVAAKLQPAGGISQLHFDQTDVSGRYEIRIGPPLARESSFAANTDPAESDLAKLD
                     RAGLAELLPGWNFLYLTNSRELTTDASSVRSRGELHRPLLYGLLILLLIESLLAWKFG
                     HHDSSS"
BASE COUNT         2381 a         4179 c         4212 g         2869 t
ORIGIN      
        1 ctcgcgcaaa atgccctggg cgatgccggg cgggtcaccc ccggcatcct ttactgtcaa
       61 gaacatgagg aactatccag gtgatcgacc ggtcagaccc gtcaaggtgc cgtttacgag
      121 cactgggccg aggatcgaaa agccccagct accgagcgct ccgaacatgt gaaaacgtgc
      181 cggcatgcgg cccacgagcg gagcctcgcc gagattgatc aacagcgtca tcggcaacca
      241 gaatatagcc atgtcgatcg catacgccgc gattcgtttc agggcataca tggagatcaa
      301 ctccccgctg tttctcgaag tgaaatggat tcaatcgact cggtcgaccg ggatgttcgg
      361 tctatcgcgg cgccgcggac gtggcaattc cacagtccca gaccgaacct tgaacggact
      421 aataatctac ccgttgcgac cgctcttgga tagaaaattt accgttaacg ttgcgaatct
      481 gaacgatcca tagtcttatt cgccgaagca ctgagcaagg gctacaactt gggtgtattt
      541 ttgcggatga gcgacttgtg catgataccg gacacaagac aaagcttgtc cgatcagccg
      601 gtcgcgacga aatttgcata catggcaggc cccattgcct ggctcttctc gggatggaac
      661 tggcaggccg tcagattgtc gcgccagacg accgcggcga agggttcggg atagtcggat
      721 tccgcggcga tatcgtcttc tactgccgcc acggcgtgat acgagtggac gaaatagacc
      781 gagggctcag ccggcaaccc cgccagtaga ggggcgggct tcttgatccg aagcgtgttc
      841 cagcccatat gcggtacttt caagcccggt cgcgccggga atcggaccac gcggcccggc
      901 aacaatccga gccccacgtg ctcaccgtct tcgctgctcg tatcaaacaa gagctgcagg
      961 ccgagacaca cccccaggca cggtttgccc ttatgcacgg cttcacgaaa cgcttcgccc
     1021 aggcccgtcc gccgcagctc ggccatggcg tcggcaaacg caccgacgcc cggcaggatc
     1081 accttcgacg cttgcgccac ccgatgcggg tccgaggtga tttcggcagc actaccgacc
     1141 gcctcgatcg ccttctgaac gcttcgcagg ttacccattc cgtagtcgat aatgacgatc
     1201 atccggcatg gtctccggtc ccggaccaca atgcgctcgc tcagacattt gacttcaaca
     1261 tcaactcaga gttgagccgc gggttttttg gcacttcgat tttcaagatg tgcggggcgg
     1321 cgacggattg tcgggcccgg ctttcgacag cggtccgatg gaaagtcgaa tggtggttcc
     1381 ctggcccggg cggctatcga tcgagagggc accgcgatgt cggcgccaga ttccttgtgc
     1441 gatggtcagg cccacgccgc tctgatcggg cttggtggtg aaaaaaggtt cggtcgcgtg
     1501 tctcaacacc tccgcgctca ttccgcagcc ggagtcgcgg atggcgacga ccagccagtt
     1561 tcgcgggtcg atgtaggtag agaccgcgac ggtgccggcc ccgccaggca gtgactcgca
     1621 cgcgtttcgt aatagatgcc cgaacatcgt ccgcagatga gcggcaagac cgggaatcgg
     1681 tggaatcgga gccagatcga cggcccagct cacctgggga ggcgccagaa ctttttcgag
     1741 ttcgacggtt tccgtgagca atttattgag gtcgatgtca gaggttccgt ggcccggtgt
     1801 atcaacgtca acggccgttg tacgtcgggt ccagtcgatc agcttgcgcg cgatttgcgc
     1861 ggcatcgagc gacgtttcga tgattccgct cgccaggctg tcgagctcgg aaggatccga
     1921 agtgtcgatg gaccgcgaac gaacgagctc ggcgttactg acgatggcac tgaaggcatt
     1981 gatcaagttg tggccaatcg ccgccgcgag atgaccgagc tctttgagct cttgcgcgtc
     2041 atcaaaacgc tgtgtatttt caccggtcat gagagtttcc ggcgcgtggc tttcaaggtt
     2101 actgcaatcg atcccggcga aatccgccgg accgatacaa cgtcgtttac gtcagtcgat
     2161 cgagtcttca gcgaatgatt cgcgcctcca cccagtcagc gaggtcgcgc acgtcgcccc
     2221 gatcaccgaa ttcggtggcc aggatcacaa acttgccacc agcgacgtca acgtcaatgg
     2281 tcttgggtga atcatgatcg gtcaagggcg gcgtttggaa gcgttcgttg ctgtcgacca
     2341 gcacgcgaaa gacgacgctc cccagcggcc ccgcccgctc gtcgactccg acaagcgcct
     2401 gaaatcttcg atcgccgggt tggagacgat acgctaccaa ggttttgctc tgcatcccga
     2461 tgcctcggtc gaacgtctgg cctgccagtt ggaaatagtg gccatcaacg gtgcgatcta
     2521 ctcgaatttc acgagtgggg ccgaaataag gaacataaga aactcggatt ggctcccgtt
     2581 ccgaaagata aacgaccgac ttgctccgga cgtgcacacg taccaactcg tttaacgata
     2641 ctttgagagc ctgtccaaaa cgcgtggtcg cgagaaccgt gctgtcatcg agcctggcct
     2701 ccgtcacacc gagacgggag ccgtccttca gcgtcagctc gagaaaatcc gaattcggca
     2761 gcggataaac gacaagcgcc ggatcgaagc cgagggcaac aacgccggcg cgatcgacca
     2821 tggcgggctt gccccccacc tgcatcgtga tcttgttttc atctatgccg aggaagcctc
     2881 cggcctgccg atcgccgttg gctagccaga caacctccgt cgtgcgaggt tcgctccgga
     2941 ctcgatccat gatcgggtcg agctcggagt cggaccgctg gccggcgaga tagagcccca
     3001 gcatgctctc gagcggaatc gccatctttc ccagcgcatc ggatcggacg tccagggtgg
     3061 tttccgctga cgacccgacc accgccctca tgaggcaatc tcccccgggg aagatgacat
     3121 gagaacggtc cagcgacgga aaagcgaccg aatactcgcg cgtcagcttg atcaagcggc
     3181 ccagcggcag ctcgcgggcg ggcccttccg cagacgcgag cttgatggca cccggtccca
     3241 gcgagacgat ccgtcccgag accgtgctcc catcaaccaa gagcgccttg aatacaggat
     3301 ccgaagccgc cccggacgac gtggcggcca gggcgctggc cccgccgtca gcgaagtgcg
     3361 tgatcgataa agcggctgcg acaagcgcac cacggtaaag ggagcacgtt tttcgaggta
     3421 agaacagcac cgctagagct cttccgttca tctttgaaag atgggcaagt aatcgttggg
     3481 aatttggagg atgataagcg ccatggccgt tccgtattcg ttgcagacac cactgtcggt
     3541 ccagaaaccc gatgccgatt gtttgtcgac cagctccttg cgaatcgctg ggtaccattc
     3601 cgcccaggcc tccttaccct ccagccacat agcctgggcc gcataatatt gaccgtagaa
     3661 gtagtggttg ttgcggcggc cgaccctcaa tccctggctg tagtgtcgga gataggcaat
     3721 cccgtcgcga acctccttcg agtcatattc ccctgcactt tgcagggcca caatgccggc
     3781 ggcggaacga ggaaactcgc tcgtcgcccc tccctggagc atgtaacgaa atccaccgtc
     3841 ggggttttgg gcctgtttca catagcgtat gcaggcatca actatttctt tgggcacaaa
     3901 gagcccggcg ttccgcgcgg cccgcagggc gttgatctgg cagatggtca cggacaaatc
     3961 ggcatcgcgg cgcaccggct gatagcgcca tcccccttcg atattttgac tatcgatgat
     4021 caggcggacg gctttctgga gcttttcgcg gatctccggc cgatgcgtca tcccgtaggc
     4081 ttcggccagg aacagcgtgc cgaacccgtg ggaatacatc gggccatgcg tcgacgttgc
     4141 gagcaccgcg ataaagcccg atggcgaagt attccgcatc acgtaggcga gcgccctgtc
     4201 gatctgcgat ccgtacggcc cacggccggg gctcgagccc gaggccatga aggccagacc
     4261 ggccaagctg gtcaccgcaa tgttgcctcg gtaggattga gtaccgtacg aaccgtcggc
     4321 gttttgattg cgcgccagcc acaagaggcc agtcttgatc gcctggtcgg tttcgggcgt
     4381 catcatctcc agagtcccgt ccggcacgtc cccccggcgt gactcaccga aaccttgggc
     4441 ggctttggtt tgatcggtcg cctgggctcg cgcggtcgcg cccaaggcca gggtgagcgc
     4501 gagcccaacc caaaaacggg ccgacgcgca atcagagaaa agtcccaggc gatccgcccc
     4561 catctgatcg ctccttctca cgaaactaaa ggtcgtctag cgctgaaaga tcggcaagta
     4621 gttgttgggg atctgcagga tgatgagcgc catcgccgtg ccgtactcat tgcaaatgct
     4681 gtcgctccag aaaccggaaa cttcttgacg gtgcaagagc tcgttacgga tggccggata
     4741 ccattccgcc cagtcgtcgc ccccgcgcag ccacatcgcc tgggccgcgt agtagtggcc
     4801 gtagaaatag tggctgtagc ggttgccgag tttgatctcg cgcatgtatt ggcgaaggta
     4861 cgtgatcccc tccttaacct ctttggtatc atactcgccg gcgctctgga gagcgaccac
     4921 tccagcggcc gacctgggaa aggcgctcgc gcccccctgg agcatgtaac gaaaaccacc
     4981 gtcaggattc tgcgacttct gcacataccg tatgcaagca tcaacagtct ctttgggcac
     5041 gaaaaggccg gcgttgcggg ctgcccgcag ggcattgatc tggcagatag tcaccgacag
     5101 atcggcatcg cggcgtatcg gctggtagcg ccacccaccc tcgacattct gggtgtcgac
     5161 aatcaggcgg acggccttct ggagtttttc acggatctcc gggcggcggg tcatcccgta
     5221 agcttccgcg agaaacagcg tgccgaaccc gtgcgaatac attggcccgt gcgtcgacgc
     5281 ggcgaccacg gcgatgaagc cggccggcga cgtgttatcc atcacgaaaa cgagcgcctt
     5341 gtcaatctgc gggccatatg gtccgcgtcc cgggctcgag ccggacgcca tgaaggccag
     5401 ccccgccacg ctggtgacgg cgatattgcc tcgataggtt ccggtgccga aggaaccgtc
     5461 ggcgctctgg gtgcgggcca gccatgccag accgctcttg atggcgttgt cggtctcggg
     5521 ggtcatcatc tcgagcgtcc cagcggggac gtcgccccgg cgggcctcgc cgaaaccctc
     5581 gaccgatttg ccctgatcgc gcgcggcttc ctgcgccaac gcactgccgc cggacatgat
     5641 cagaatcacg aaccagcaca gcgcggatga acgggcgaaa gcgcacgcgc gaactgcccg
     5701 tagggcggcc atggcttcac tcctctcgca cgagctttcc tttatctacg gagagaaaat
     5761 accgactgat cagctctttc ttggagtcga gctccgtttc cttgaagctg ttatccatga
     5821 tcttgcgtag ctcaggcggc aggtgccccc agacgtcttt gccgccggcg gttgagtgct
     5881 ggctggtggg cttggccggt ttcattgctg gagcaccgcg ggcctgcgcg ccgggttgat
     5941 taccctcctg ctggcccggc tgtcggccct gctgctgcat gcggcgcatg gccatgctcc
     6001 cggccgatcc accctgctgc tttacctgtt cgatcagggt ctcaatgcgc ttgacgatct
     6061 gcttttgctt tttctgcgtg tcctcgctgg gatcgggttt gcccagccgc tgttcgactt
     6121 cgcgcatttc cttgatcata tcgccgatgg ggccggtgcg ctcctgttcg gaggacgggc
     6181 gttttttctt ccgcccggtc aactcctcga gccgctcgtc cagatccttg tctttagctc
     6241 cgagcttatc gccacctggt ttctggccgc gcgatggctc agtcggcccc tgaccgggag
     6301 cccgattccg gggctctggg gcggcggttg gatcgtcctt cgattcaccc agttttccga
     6361 ggagatcgtc gattgcttga tccttggggg ccagcttggc ggcgtccggc gacttgccgg
     6421 cggatggctt gtcgctcttg gctgcgggcg cttggccatc cttgtcgccg gtcgccttcg
     6481 ctcggggctc aaccttcgat ttgtcttggg gccgggtcgg cttctgggtc tcggctgtcc
     6541 caggcttggc gggttgcgcc ggggccttct cggactctcg gagctctttc atcagcgaat
     6601 cgagcgcgtc gtccttcgat ttggcggtat cctgggccca cgcgcgatcg ccgcccatta
     6661 tggcggcgag gatcgcgaac ttgaggatga actttgcgga tttgagcatc atcgattact
     6721 cctccccgtc gtcccgtttc ggtcgggtca tatcacgaac caggtcggct aataccccct
     6781 gctcttcgcc aagccgcctg agctcggcgt cctgatcgga ggtcagcttc tggttgcggc
     6841 gctgaacttc gtcgagcgct tcggtccgct cgttgatttc ttgttgaaga gacttgagca
     6901 tcttgagctg ggcggttgcg gggatgccgt cgccgccgcc gttaccgcct gctccaccac
     6961 caccgccgcc gccccctcct ccgccccctt ggccgccgcc ggcagagtcg gacttgagcg
     7021 attcgaggag ctgcttgaac cgatcggacg ctgctttggc agcttgttcg gtgaggccgt
     7081 cggtcttgag ctccgccagc cgcttggcgg cgttttccat actctctttt gcgcggcgga
     7141 gagtgagggc gaacacgggc gccccatcga ggttttcaat aagttcggcc gtctcttcct
     7201 tgaggcccga ctggacctgg cccagccccc tgacgcctgt tcgctgcgcg atcgagagct
     7261 tgccgtcgtt tttctggcgc atggcttcaa aagagctggt ctccgaaacg accttcgcct
     7321 gccgttcggc gagccccttc aaacggtccc ccatccggac gagctgttcc atggcaagtt
     7381 gttcctctgc gtccttgcgc gtttgctcga gctcttcctg ggcgtcgtcg agatcggcca
     7441 gggcttcctc ctgttccttg ccggcctgtt cgccctgatc gtcatcgagg tcgccctggg
     7501 cgcggtccat cttgccgctg gcggtctggc ccttttgggc ggcgcgatcg gcactcagtt
     7561 tcgccagctt ttgcagttgc cgcttcaagt ccttttgaat ctcggcttgc tctttagcga
     7621 gccgtttgag ctggtcggca cgctgcttgg cgtcgggatt gcgctgggct tcacgggtct
     7681 ttttcaagtt ttgtgcctgg cgggcgcggg tgttggccag ctcggcctcg gcgttcttga
     7741 gctctttgac aagtctggca agctcgcgct cgcggcggtt ctggatattg tcgaccaagt
     7801 ctctcagctc ttcgcgtgcc tgctcctggc gagcctgggc ctggcccatc tggtttttct
     7861 caagctgatc ggccgcttcg gccagcttgc cggcggttcc tttttggcgc gatttctcgg
     7921 cggcctcgcg catggccgat gcggccatcg ggtcggactc gtccatgcgg cgagccatct
     7981 cggccatccg ttcctgcagg ttttgcagcc ctttgcccac ctgcgattga cgagaggcca
     8041 gattgccgag atcggctttt tgttcctggt tgagggcatc gaggggcttt cccctcgtat
     8101 cgggcttgtc ggcggcttcg tccgtttgct tcatggtctg ctcgtgctgt ttgagcagtt
     8161 cctgagcgtc tttgaccaca ccgcggtagg tttcaaattc gctcaggccg tcgagcattt
     8221 tctgcagttc attagcaatg gccttctggt tcttattgga ttcggccaga gcaagttgtg
     8281 tcgattcctt gccaggctgg gtcggagagg ttttcgatgg ggtctcgggc ttggaagctt
     8341 ccttggccgc ttcaccactt cgttcgttcc ccttcgatgc atcaccctgc tcgttctttc
     8401 ccggtgaccg atctccgcgt tcgttgcccg tctcggccgc gctctctaac tgcttggcgg
     8461 tcgcaggttt tgcctcagtc gctttcgcgg ccggcgatgg agacgcgccc ttctgcgtct
     8521 ggtcttttga gtcgggctgc gcctgatcgg ccgcggccgg cttgttgtcc gctggattcg
     8581 cggcgggcga cggttgagcg tccttggttg ccgcggcttc gtcctccaag gtcttggtcg
     8641 cgcgggccaa ctgttgctcg gccgggccta agtgctggtc gcgaatcatt cccagccgcg
     8701 cgagcatgtc ttccatctgc ttctgggcat cggcgttgtc gatcttgaag tttttcagat
     8761 cgtcgagcac gtgcgagatc cgcgcgccga ccccttcgtc gcgattattg atgcggctgg
     8821 tgacctggcg ctggatcatt gtcccgttct tgaggtcgtc gcgctctcgg gcagccagac
     8881 gatcggtctg cgacaaggtc cgcacggcgt tgtcgaccgg cgtgatcgct tgcttctgca
     8941 tgttgaggac acgcgacagc tcgtcccgca gctcgcgccg cgcgtcgtcg aactggcgga
     9001 ctgcgtcttc cttggaaacg attcgcaggc gaatctcgcg gctcttgccg acgtttggtc
     9061 ccttgatcgt gtcgaaatcg cgtgcgttgc cgtagaaagt gatcacggtc cccacctgga
     9121 gcttgagcgg ggcgagttcc caccggtgcg atatttcctg gtgtttggtg aagcccgcgg
     9181 cggcgccttt tggttcctgt tcctgagccg accagagagg aatggcgact tccgcatgcg
     9241 gctctgagtc gccggtcgcc agcgtgtaga tgagccgcgc ggagtggagg ccgaagtcgt
     9301 cgtcgagggt tatgcggacg ggtatcgtgg agtccgccgg aacatcgcgg tcggccctag
     9361 gttcctcgat gacgacgcgc ggggcctcgt cgcggaaccc ccggacgtcg tagcggactg
     9421 cctcgcggtt gcgaaatccc tcggtgtctt tgagatcaaa ccagaatccg aaagtgccct
     9481 ggaccggcag cgtcgtcttg aatcgggtcg cacttgtgtc gaacgttaac gccgtgcccg
     9541 cctcattttc gccctggcgg agctctgcgt gggccagcgg cttgttcgca ataccatcca
     9601 attcaagctg ggttccttcc agggcgcgga gctgtgtcag gccgggggca agcgcttgct
     9661 cctttatgcc ggtgtacgtc ggagggatca gtttgatggc gagcgacttc aaggtggggg
     9721 gagggacgac cttgacttcc acgtcacgga tcgaggtgga atcgtcgcct gcggcgaccg
     9781 agaaccggaa tggctgattg accgagtcga tccgtccccg gaactcccct ccctcgatcg
     9841 accgcattgg ctcgccggcg ctttcgccgt cggcgaacag ataggtcgcc tcagccgatt
     9901 cggggacctt gtcgccgggg cgaaccttga cagccagagt gaacgagtct ccccgcgcga
     9961 ccttgagggt ggtcaggcct tcgtccagaa tcagatgggt ttgtcgcggc cagctcgtcg
    10021 atccaaacgg aacaaacagt cgtttcaggg cgatccgtga cgttaccggt gcaatcagaa
    10081 gcaggagtgc tcccagtgcc aatgcgctgg cggcgacgcc cagcgcccgg acgaccggct
    10141 tgggctcgat cacctcgcga aagtcgattt tgctcgcttc ctcgaccgtc tgtcgaacgg
    10201 tcgcctcgcg cagggcctgc gagccgaagc gcttgtcttg ggcatcgagg cgaacgaact
    10261 ggatggtgct cgccagccgg tcattgaaac cgggccaccg ttcctcgatc cgcatggcga
    10321 tatcgaggtc ggcaaacttg acgaacaggg ggcgaagaac gcggcgatat ccgaggtaga
    10381 tcaagccgcc cgccaaagcc gccagcaggg tcgctcggat caccgagtcg agctgaaacg
    10441 cccagtcggc gaagcccgcc acgaccacga gcggaacgag gatccccagc acccacgaca
    10501 gcccatggag cgccagcaac cgcctgagag aaccacgtaa ggccctgatt cgcgattcca
    10561 actgacgagg agtcatcgag tcctccctag ttgccggttt ccgctgagtg gtccgagttc
    10621 caatggaatt agtaacttac gactactata cgaatcatac catctgcttg cgttttctga
    10681 aaacccactc cgtcgtgatc aagagtagaa acaatgtcag gatcggccag gtgttccaga
    10741 gcggaatggg gggatcggta tcgagtggga ccttggaggg tttaggaagg tccttcaaga
    10801 gcgtgacggc ggtgaggggt gtgtagaatt tgccgccggt tgactctgcg acgcggatca
    10861 gttcgggctg gttcatttcg atgcgttcga actcgttaac cggcgcctcg acgcggaagc
    10921 tggtggaggg aatcgggccc tcgagtaccg gcggcggcag gagccggacg tcgtattcgc
    10981 cttccggcgt ttgcgccagt gcaccctcga agacatttct ggtgccgggg acgagctgaa
    11041 gcgcgagctt gcgcgggccc tggcctcggc gttcgagctg aacggcgatg tcgccggtcg
    11101 tcggagccag tcccggattg gggaagcgga cgcgaaactg gattggctgt cctctttggt
    11161 accgtttgcg atcggtttgt acctcggcct ggcgctggcc gaccagcttt gatcgggcca
    11221 tgaaccggat cgtctggacc cagaagcgtc cgaaatacct gtcgccgacg cgaaaccgcc
    11281 agcgccaggt gtcgtcgaag gcatggaaca tcgccttgcc cgcgccaaca aagtggtaga
    11341 ggaccagggg gagcttgccg tcagacccga ttgccgtcgg gtgttcggcc agtacgagcg
    11401 ccgccggctt tttgcgcgga gcctccaggt accagaacaa ctcgggaagc ttctgccaga
    11461 tttcgatgct cgcggcttca ctgtcgccga agcgaaagat tgggcttgac cgtccctcga
    11521 gcgtcagctc ggggcggaac gaggtgatgc cggtgcccac ggccgtgggg ttgcgtgcgt
    11581 cggccagctc gacaggcaag agcacctcga gcgggctgcc gcgatacgac aatgggttaa
    11641 acagctcgcc cgcgacgaag agcactccgc cccccttctc gttgacgaac tcgaacaggt
    11701 tttgcatctg cgactgactg aggaagctgg tgtcggcatc gccgaaaagg acgacgtcgt
    11761 aggcgaacag gtcatccttg gcggcgggaa atgtcggcag tgcaatgcgg tcttgctggc
    11821 tgtacgcggg atcggacgac agcagaacga tgttcaaatc aatggtctcg tcgcgctcga
    11881 ggtagttctt caaatagcgg aactcgtagc gcggctcgct gtcgacgaac aggaccttga
    11941 gcttttcctt acggaccgtg accatgcgct cgatcagatt gttctcgatc tggagctcgc
    12001 ggggtttttt atcgacttcg aggatgtacg tccgctcgcc agtggtcttg gggtgatgga
    12061 caagctcgac ccgtttaggc tcgccgttgg ggggagcatc gacctcgact ttttcgagtt
    12121 cgcgcgccgt cttgggatcg cgcgaccccg gctcaagctc cttgagccga acgaccactt
    12181 tttccccctg gaagccccgg gccagcagct tggcctggaa tcggacggtg tcgtcgacga
    12241 agaccacgtc atcgaccaag agttcggtca gctcgatgtc gcgggcaggc tcggcgcttc
    12301 ccaggccgac ggcaaagatg ggaacgccct tgcgtgcggc gagcgccgcg gccttggata
    12361 gaggctctcc ctctgtggtc tggccgtcgg tgaataagac gatggccgag gggggtgaac
    12421 cgcggagctc cgtcaagacg tttcggacgc cgtcgcccaa tcgtgattgt gatccctgcg
    12481 gcaggatccc ccgcacacgt tttactgcct cgtcaaggtc ccgaggtcga tcgacgtcgg
    12541 cgaggttacg ggtcgcgctc gagaccagat aaagccggac cttgtgctgt ttctccagtt
    12601 cggagagcag cttggcctgg tcctgcaaga tcaagccctt ggcgatgtcg agccgggtcg
    12661 tctccttgtc gttagtggtc gtagccgtcg caccgacgcc atccggcccg gatagggctg
    12721 ccagctcgtc aagcgctttc tggacttcgg gtttctcata ctgatcggcg atgcgctggc
    12781 tgtccgagtc gtcaatcagg atcgtcacgt agggaagccc ggtccgctcg acggatagaa
    12841 cggcctcgga gagcatgaac atggccagaa acacaatcgc gatccgcagc ccggccagga
    12901 aaatcttggt cgcggtcgac gcctttccct catggcggta aagccagacg atcaaggcca
    12961 cgctcaagac cgccgtgaag acgagcagcg tctgatccca cggttgttcg aagcggattc
    13021 tgggattggt tgcctcgcct gcctgggggg gtgccactcc gagtcgatcg gccaagcctt
    13081 ggaggagcca gtcagttact ttcaagccac ggagccagtc ggtcacgttc aaagcacctc
    13141 aggacgagga atcatggtgc ccgaatttcc acgccagcaa tgattcgatc aagagaagga
    13201 tcaagagacc gtacagcaag ggccggtgca gctcgccgcg actacggacc gagcttgcat
    13261 ccgtggtcag ttcgcgcgaa ttggtcagat aaaggaaatt ccagcctggc agcaactcgg
    13321 ccaatcccgc gcggtcgagc ttggcgagat cgctttcggc gggatcggtg ttggcggcga
    13381 aactcgattc gcgcgccagc ggcggcccga ttcgtatctc gtaccggccc gatacgtcgg
    13441 tctggtcgaa gtggagctgg ctgataccgc cggccggttg cagcttggcc gcgaccggcg
    13501 ctcccctggg tggcaccacc gtgacagggg ccgcagcccc ggccgacgga aacgactgat
    13561 cgaacggctg gccgacgcga atattgcgct ccgacatccg gccggccgcc gccctcagca
    13621 cgatctcctg catgaccggc g
//
LOCUS       DATAQL010001408         6567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1365369, whole genome shotgun sequence.
ACCESSION   DATAQL010001408 DATAQL010000000
VERSION     DATAQL010001408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365369"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VHS97_24285"
     CDS             complement(<1..349)
                     /locus_tag="VHS97_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HEX3451394.1"
                     /translation="MATVVEAPIEVLPNEPEANKLFRMVMKFKGSDLHLKVGMAPAMR
                     LAGVLRIMQLPPLSITDMERLMFPLLTPRQKDILEETGGIDFAHIIFEPNGDETRFRV
                     NLFRQRGRLSLVAR"
     gene            complement(451..1299)
                     /gene="hisF"
                     /locus_tag="VHS97_24290"
     CDS             complement(451..1299)
                     /gene="hisF"
                     /locus_tag="VHS97_24290"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX3451395.1"
                     /translation="MLAKRIIPCLDVNQGRVVKGTNFVNLKDAGDPVAVAARYDQEGA
                     DELVFLDITASHEDRAILLDIVRRTAEVCFMPLTVGGGIRKLDDIRTLLSAGADKVSI
                     NSAAVRDPELIRQSARRFGSQCVVVNIDPKRVKRDGRLMWEVHINGGRVPTGKEAVSW
                     AREVEELGAGEIVLTSMDADGTQNGYDLEMTRAVADAVAVPVVASGGAGRPEHLRAVL
                     ADTGASAALAASIFHYRHYSIKETKEYLASRGVPVRLVEPALAEEAGQGWAVAGTKAG
                     SRVFNQ"
     gene            complement(2189..2563)
                     /locus_tag="VHS97_24295"
     CDS             complement(2189..2563)
                     /locus_tag="VHS97_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX3451396.1"
                     /translation="MGKKLYVGNLTYGVSDSTLEQLFSQFGTVQSAQVIQDRATGRSK
                     GFGFVEMDTEAQAQAAIEGLHDQEHDGRRLTVNEAKPREDRGGGGGYGGGGGGSRGGR
                     GGGGGGGYGGGGGYGGGGGRRY"
     gene            2963..3874
                     /locus_tag="VHS97_24300"
     CDS             2963..3874
                     /locus_tag="VHS97_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX3451397.1"
                     /translation="MIAVAWVPLLVFLTTLNYQERPTTSAPEQPPAVPQSPVDAPASP
                     TAPEKNSSKSPEKKQSGPSRKSPAARAKMPDTAKSRSTTPVAPEEVRVRSQAEMRKPK
                     SPFLLAPGGSPQDRYGPDADTDWTEVPPWRQTSFFGVRARGLFFVYVVDCSGSMIDDD
                     RMPRATIELRRSVLALQSPQKFEVIFYNVESIPMPGGPIARSADLQAKNQLMTWLRQI
                     EPDGGTDPRVAVKQALWLRPDAVFLLSDGALPEGTADQIAKLNTKKIPIHCVDLAGGL
                     GGDHLKRIAQASGGQYVSRPGDMQGRP"
     gene            3959..4684
                     /gene="msrA"
                     /locus_tag="VHS97_24305"
     CDS             3959..4684
                     /gene="msrA"
                     /locus_tag="VHS97_24305"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX3451398.1"
                     /translation="MNYRTSVIVCMGILLGLFAALYLGGTYVKPNSSPETATALKPAA
                     ATLLKPEATKAVKSAETPAGMATATFGAGCFWCTEAVFQRLKGVHSVVSGYSGGSVQN
                     PTYRQISTGATGHAESIQVTYDPAVVSYEELLEVFWQTHDPTTQDRQGNDVGPQYRSV
                     IFYHTDAQKKLAEEYKQKLDHSGAFDGPIVTQIVPFTEFFRAEDYHQNYYNKNSQQPY
                     CRVVIGPKLAKLKKVFQTKLKSE"
     gene            complement(4733..4999)
                     /locus_tag="VHS97_24310"
     CDS             complement(4733..4999)
                     /locus_tag="VHS97_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451399.1"
                     /translation="MATVILGWVLTVVGVLFIFLGLVAASRQVRVTTASAPKPGIRGV
                     LHDAAVLLDALRKLPLWLALTIIGIALVTVGQMLVHGWPSALPP"
     gene            5617..6321
                     /locus_tag="VHS97_24315"
     CDS             5617..6321
                     /locus_tag="VHS97_24315"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3451400.1"
                     /translation="MSNVPNIDNSAKAPEAVPSRRSFLTRAAGVSAIAVPTLTLITSR
                     KARAAVSKKLTGLNAELINEVMDDEDAHVPILQGLLNDPDNPLPVPIRKTPNLNFVAL
                     RQPNLQAFLETASAFENTGSGLYHGALLNITQTQEYFPIAAGLAAVESRHASWLNSLL
                     GESLVPDFAPVEAPIAQSTVLSRVTPFVTDPRATFPSFDTTVVSDANNFFILDFVLFL
                     EYIESAFYNLNVPLFT"
     gene            complement(6310..>6567)
                     /locus_tag="VHS97_24320"
     CDS             complement(6310..>6567)
                     /locus_tag="VHS97_24320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451401.1"
                     /translation="RADHHRERRRCPQETPQFHVHHIVFHLMTALGADPRFEETPERG
                     GNEHCKMTQREVLATLTPMIGISALCDSGADADRSALFSRE"
BASE COUNT         1362 a         2040 c         1891 g         1274 t
ORIGIN      
        1 ggcgggcgac caggctcaat cgacctcgtt ggcggaacag attgacgcgg aatctcgtct
       61 catcaccgtt gggttcaaag atgatgtggg caaagtcgat cccgccggtt tcttccagga
      121 tgtctttctg acgcggcgtc aagagcggaa acatcagccg ttccatgtcg gtgatcgaca
      181 gtggcggaag ctgcattatc cgcagcaccc ccgccagccg catggcggga gccatgccga
      241 ctttcaagtg caagtccgag cccttgaact tcatcaccat ccgaaaaagc ttgttggctt
      301 cgggctcgtt gggcaaaact tcgatgggtg cttcgacgac ggtcgccatg ggtgtgcact
      361 cctgctctag caaagacttg aagatcacct caattggccg ctaaatcgtc ccctactcgc
      421 cgcgatacac ttcaattccc ggcggtcgat tcattgattg aaaactcggg agccggcctt
      481 tgtgccggcc accgcccagc cctgaccggc ctcttcggcg agggcgggct cgacgagccg
      541 gacgggcacg ccgcggcttg ccagatattc cttggtttct ttgatggaat aatgccggta
      601 gtggaaaatg ctggcggcaa gggcggcact tgcccccgtg tcggctaaaa ccgcccggag
      661 gtgctcgggc cggcccgctc caccggaagc caccaccggg accgcaaccg catctgccac
      721 cgcccgcgtc atctccaaat cgtagccatt ctgagtaccg tcggcgtcca tgctggtgag
      781 cacgatctct ccggccccca gctcctcgac ctcgcgagcc caagagaccg cttcttttcc
      841 ggtcggaacg cggccgccgt tgatgtgcac ctcccacatc agacggccgt cgcgcttgac
      901 cctcttgggg tcgatgttga cgacgacgca ctgactgcca aaccgccggg cagactggcg
      961 aatcaactct gggtcgcgca cggccgccga gttgatcgaa accttatcgg cccccgcgct
     1021 caaaagcgtg cggatgtcgt cgagcttgcg aataccccca ccgacagtca gcggcatgaa
     1081 acagacctct gcggttctcc ggacgatgtc caggaggatc gcgcgatctt catgactggc
     1141 ggtaatgtcg agaaacacca gctcgtcggc tccctcttgg tcgtaccgcg cggccaccgc
     1201 gactgggtcg cccgcgtcct tgaggttgac gaaattcgtt cccttgacca cgcggccttg
     1261 gttgacgtcg agacagggaa taatccgctt ggccagcatg cgcagtctca acccattatg
     1321 ccgtcggcgt caatgctcgc cagcaaaacg ttcgtgtacc cgaaccacga attatcagaa
     1381 cccgaaggaa tagactgtat tctataaaag tgcagagagc gatcctagag gttgtcaaga
     1441 agcgattatt ttgagcctcc acccccgtcg ggccatgctt atgaagctgg cgtcaagtaa
     1501 ctagtgtggc gtcaacctgc gattgatggg tcaactcgcg ggggtcgttg tcagttcgca
     1561 ttctttgcgc cattagccct tcggaaagac cagccgtcaa tcgaaggttg gcgggacact
     1621 agagcgcgcg gtgccgcatt cgacgctggc aaagcgcgat tcgcgctggc cgcaactggc
     1681 gcgtataacg acaccatcgt gccgcgtgtc gaagcttttg gttggcttca cgctgaaggg
     1741 cgatgaccgc gacatgagac ccgagacagg accgcttcag acgatgtgtc agggatcgat
     1801 caaggtcgca tcgatcacgc tgtcgacgat ggcgacgccc acgccttcac cagccggaac
     1861 accaaccttt gatttgtcga tcttgggcat cgcaggctcg gactggcgca tcgcgcattg
     1921 tggatgaatg ccaccccgcg agtagacagc ctgatgacaa accggacaac gaagccgagt
     1981 ggacaccggc gtatggaagt gtttgggatt catccggtcc gtccggtagg tagaagagaa
     2041 atggcgcgcg caggcgtgcc gtcagtgggt ggcgagcttg agagatggcg gtgcccgtaa
     2101 agccgaggtg catgtgccgc gctctagaga acgcggcgtt ttcgcgaacg gcagtccaag
     2161 acatccgcca ccgggagcca acctgcgatc aataacgccg gccgccgccg ccgccataac
     2221 cgccgccgcc gccgtagccg ccgccaccac caccgccgcg accaccacga ctgccgccgc
     2281 cgccaccgcc gtatccgcca ccaccgccac ggtcttcacg cggcttggcc tcgtttacag
     2341 tgagacgccg tccgtcgtgc tcttggtcat gcagcccctc gattgctgct tgagcctgcg
     2401 cttcggtgtc catttccaca aagccgaaac ccttgctgcg gcccgtcgcg cgatcctgaa
     2461 taacctgagc gctctgcacg gttccgaact gagagaacaa ctgctccaga gtggagtcgc
     2521 tgaccccgta ggtcaaattc ccaacgtaca acttcttgcc caccgctagt ctcctgggcg
     2581 aatccaaacc gacccatatc gtctttccgg gtcgcagaga aaggggctgc gagacagccc
     2641 tgtcggtggg aaaggcgagg gagggaacaa ggtcagggct cactgagcca gaactgctct
     2701 cttcagtcga cagacctacc aattcggtat gttagccgaa ttttcgtcta aatgataggg
     2761 gaaattgact agagcgactg cttggctccg agtcaaggga cgcgagctcg cccacctggc
     2821 ggcagcggcc agtccgtcac tgcggaagtg cgccagtcgg ccgcctgtct tcgatcgatc
     2881 gacttgtgat aaactcagat tggatccggg cagtcgggga gcggccaagc ccggcgtgtt
     2941 cgctgtcatg gaaatttgtg gggtgatcgc cgtggcctgg gtccctcttc tggttttctt
     3001 gaccaccttg aattaccagg aacgccccac cacctccgcc ccggaacagc cgcccgcggt
     3061 gcctcagagc cccgtcgatg caccggcatc gcccacggcg cccgagaaaa actcgtcgaa
     3121 gtcgcccgag aaaaagcaaa gcggaccgtc gcgcaagtcc ccggcggcca gggccaaaat
     3181 gcccgacaca gccaaatcac ggtccacaac tcccgtcgcc ccagaagagg tccgcgtccg
     3241 gtcgcaagcc gagatgcgca agcccaagag cccgtttctt cttgccccgg gcggttcgcc
     3301 gcaggatcgc tacggccctg acgcggacac cgactggacc gaagttccac cttggcggca
     3361 gacgtcgttc ttcggcgtta gggcgcgggg cctgtttttc gtgtacgttg tagactgctc
     3421 cggcagcatg atcgacgatg accggatgcc gcgcgccacg atcgagttaa gacggagcgt
     3481 cctcgcgctt cagtcgcccc aaaagttcga agtgatattc tacaatgttg agtcgatacc
     3541 gatgcccggt gggccaattg cccgctctgc tgacttacag gcaaagaatc aattgatgac
     3601 ctggctgcga cagatcgagc ccgacggcgg aaccgacccg cgtgtcgcgg tcaagcaggc
     3661 gctttggctg cggcccgacg ccgtattcct cctgtccgac ggagcattgc ccgagggaac
     3721 ggccgaccag atcgccaaac tcaacaccaa gaagatcccg atccattgcg tcgatctcgc
     3781 cggcggcttg ggcggcgacc atctcaagcg gattgcccag gcgagcggtg gccagtatgt
     3841 ttcgcgaccg ggagatatgc agggtcggcc gtgaggcggt ttcgtcgtgc aatggggact
     3901 ctcccgcgac gattccccga cgacgcccaa tcggaaacca tcgcggagga ggttgctcat
     3961 gaactaccgg acgtccgtga tcgtgtgtat gggaatactt ctgggtttgt tcgccgccct
     4021 ttacctggga ggaacgtacg tgaagcccaa ttcgtcaccc gaaacggcaa ccgctctcaa
     4081 gcccgcggca gcaacccttc tgaagcccga agcaacaaag gcagtgaagt ccgcggaaac
     4141 gccggcgggc atggcgaccg cgacgttcgg cgcgggctgc ttttggtgta ccgaggccgt
     4201 gtttcagcgg ttgaagggag tgcactcagt tgtatcgggc tatagcgggg gctcggtgca
     4261 aaaccccacc taccggcaga tttccaccgg cgcgaccggc cacgccgaat cgatccaggt
     4321 gacgtacgac cctgccgtgg tctcgtacga agagcttctg gaagttttct ggcagacgca
     4381 cgacccgacc acgcaagacc gccagggcaa cgatgtgggc ccgcagtatc gatcggtgat
     4441 cttttaccat accgacgcgc aaaaaaagct cgccgaggaa tacaagcaaa agctcgacca
     4501 ttccggcgca ttcgacggtc cgatcgtcac acagattgtc ccgttcaccg agtttttccg
     4561 cgccgaagat taccaccaga actattacaa caaaaactcg caacagccct actgccgggt
     4621 cgtgatcgga cccaagctag caaagctgaa aaaagttttt caaaccaagc taaaatccga
     4681 gtaagagact caggaccctt aaacgcggcg atttcgctcg acatggtgac tctcacggcg
     4741 gaagtgccga cggccagcca tgcacgagca tttgtccgac ggtaaccaat gcgattccga
     4801 ttattgtaag tgccagccag agaggcaatt tgcggagggc gtcaagaagt acggccgcgt
     4861 catgcaagac acctcttatc cctggcttgg gcgccgacgc tgtcgttact cgaacttgtc
     4921 ttgaagcggc caccaatccc aagaatatga agaggacgcc aacgaccgtg agcacccatc
     4981 ccaagatcac ggtcgccatg cacgagctcc tcgacctgga tcctgttgtg cggcggccca
     5041 cctcggccgg ctcggcacgc gtcggcgacg atcataccga aatcatatcc gcggaatgaa
     5101 gatcgagccg atcgcatctc cggccccgcc gctggccgaa ttgggacggg cagaaaaaag
     5161 cgtaaccaag ccgacgcttc aggtctcttg tcatgagaaa agcgggccga ccgtgggtcg
     5221 cagcgaagcg ttaggtgcac aagtaggtcg ctggcgatcg acggtggagc cttgatgctc
     5281 gctcgtttcc tcgcgtgtgg agaaacgcga ccgacctggc gaccccggtg aatgaacgtc
     5341 tcagcgattc gccggacgtg acaggccgtg gagtcttttc gcaaccgccc gaaacccggg
     5401 aaatgggccg gggcgattga tcggcattat cctggccggc acgcatccgg actgggtcag
     5461 gctgcgtgcg caattaatgt gcatcacttt atagtattta tatcattatt cagtaagtca
     5521 ttggacctgt tgggtattgg gagcaatgtg tcctcaccgc cgggtccgat tccggtgtcc
     5581 gtccgtagcg atagatcacc ctaggaggca gtatccatga gcaatgtgcc caacatcgac
     5641 aattctgcca aagcccccga ggccgtccca tcgcggcgtt ccttcctgac ccgcgctgcc
     5701 ggcgtgtcgg ccatcgcggt gccgactctc accttgatta cttcgaggaa ggcccgcgcg
     5761 gcagtttcca agaaactgac agggctgaat gcggagttga tcaacgaggt catggacgac
     5821 gaagacgcac acgtcccgat tcttcaggga ctcctcaacg atccggacaa tccactccct
     5881 gtaccgatcc ggaagacgcc gaatctcaac ttcgttgcac tgagacagcc gaatcttcag
     5941 gcgttcctgg agacggcatc cgcgttcgag aacaccggat cgggactcta tcacggtgcc
     6001 ctgctcaata tcactcaaac tcaagagtat ttcccgatcg cagccggact tgctgctgtg
     6061 gaatcgcgtc atgcctcatg gctcaactcg cttttgggtg aatccctcgt gccggatttc
     6121 gccccggtcg aggcgcccat cgcgcagtcg accgtgctgt cgcgggtcac accatttgtt
     6181 accgaccctc gcgccacatt cccgtcgttc gacacgaccg tcgtaagtga cgccaacaac
     6241 ttcttcatcc ttgatttcgt gctcttcctc gagtacatcg agtcggcttt ttacaatctg
     6301 aacgtcccgc tattcacgtg aaaaaagagc cgaacgatcg gcgtcagcgc cgctgtcaca
     6361 aagggcgctg atgccgatca tcggcgttaa ggtcgcgaga acctcgcgtt gggtcatttt
     6421 gcagtgctca ttccctcctc gttcgggcgt ttcctcgaaa cgtggatctg cgccgagcgc
     6481 agtcatcaag tggaaaacaa tgtgatgaac atgaaattgc ggcgtctcct gtgggcaacg
     6541 gcgacgttcg cggtggtggt cggcgcg
//
LOCUS       DATAQL010001409         5740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1669095, whole genome shotgun sequence.
ACCESSION   DATAQL010001409 DATAQL010000000
VERSION     DATAQL010001409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669095"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VHS97_24325"
     CDS             complement(<1..218)
                     /locus_tag="VHS97_24325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX3451402.1"
                     /translation="MKHITIGLIGLGTVGTGVARLLTEHPDRIARRAGRRVRWAWAAV
                     RDLEKPRACRLDGVWLTTDAAEVIDDPDV"
     gene            complement(230..1003)
                     /locus_tag="VHS97_24330"
     CDS             complement(230..1003)
                     /locus_tag="VHS97_24330"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX3451403.1"
                     /translation="MPSPFPGMNPFLEQDDAWHDFHEKIIPMIAERLVVQVRPDYIVK
                     IDEHIYVHELPPEPRRYLGRADVSVSRSEDITGRSGIGLLGAPTEVRLPVHDVEQLAF
                     VEIRDRRSRELVTVIEVLSPSNKQSGSSRDQYLAKRQELLDSRANLVEIDLLRGGKPM
                     PLDERPDRAYAYSVLVSRVDDRPIAGFWPIGLRNRLPVIPVPLRAPASDARLDLQEAL
                     NHVYDASGYEDYIYSGLPDPPLDQEDKAWAESLIPRLQA"
     gene            complement(1071..3683)
                     /locus_tag="VHS97_24335"
     CDS             complement(1071..3683)
                     /locus_tag="VHS97_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HEX3451404.1"
                     /translation="MIETHEPVTEPSAVTHALGGVSRQLGLRSRVKPELLAPAGDRTC
                     LIAAVENGADAVYFGLQRHNARIRASNFDGTDLPEVMALLHRRGVKGYVTLNTLVFPD
                     ELTSLEETIRDLATAGVDAAIVQDLGLVRLIRAITPDLEIHASTQMSITSEEGVALAR
                     ELGCTRVILARELALAEIDKIRSAIDFPVEVFVHGALCVAYSGQCLTSEALGGRSANR
                     GECAQACRMPYQIVCDGRLVDLDNIQYLLSPQDLAAYDLIPRLIDLGVASLKIEGRLK
                     SAEYVANITRHYREAIDAAWAGHPVAFTRRDVEEMQLSFSRGFSHGFLDGTDHKVLVR
                     GDYAKKRGIPLGVVESVSSSGVRLKVSAQVKPGDGLVFDGDERAGRAEQGGRVYEVIA
                     LDRTGKNAGSRVKGGGEQGHVELRFGRGDIELRALEIGQRVWKTDDPELTRRLRRSFE
                     GPPSRKIELTLEVFAVTGERLRLSGRAPLGLRASVQSADVLGPAASRPADLELFQAQL
                     GRLGGTSYELIDIEATIEGRPMVPMSLLNQLRRELIARLDELASAVPARAIAIEPVLP
                     TLLGPIARERDRQLERLRNRYQPLELSVLCRNTEQIEAAVTFGVSTIYAEYQDIKQYA
                     QAVAAVRRGTARAAIYLATPRIEKPGEANLFSYLAKQGADGILARNAGGMKFCSERGI
                     PFVADFSLNAANPLSVELLKSRGAVRVTASYDLNVDQLMDLIDSTPPEWIEVVIHQQI
                     PMFHMEHCVYCAFLSPGTDATNCGRPCDTHDVKLRDRVGMDHPLKADVGCRNTLYNAV
                     PQTAVEYLPRLQKHGARFLRIEFLDDSPATVERTITLYREVIAGEREGKALWRELKAR
                     NQYGVTRGPLAVIT"
     gene            complement(3925..5403)
                     /locus_tag="VHS97_24340"
     CDS             complement(3925..5403)
                     /locus_tag="VHS97_24340"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEX3451405.1"
                     /translation="MSYFARLWGRDSRRRPVPARYVLEALEGRTMLSGTGATTAASIS
                     SQTTIYTNFPTVVAGAQVAFLGVVQNASTGAPITSGNVTFVAESPNKIVLGEASVNSQ
                     GQATIVTSDLTTIGSYQIEAQYTPSNSSVSASVSSPETTNVIPLPLDVPTATTVVSGA
                     ASAEAGQSVPLLATVHDAGTGDQVNAGKVEPISGTVEFVEDSPQPVVLGKVTLNKNAQ
                     AALSTNMLKNVGPYQIEADFVSANNYFAGSNSARTAMTITPTTVNAPTVTSLTAVTPS
                     VETGEPMTFSASVQNTNSSLADGVVEFVTVGRHPVVLGKVDVGTFGQQLSLTTSKLEK
                     VGTYQIEAKYLPNTNRFAESASPPVNVTVTPLRPASFRVTPTLRRAHINVPLSFTVTA
                     VDAQKQPVTNYTGTVLLSSPTDSWTTFPTAVYRSLKTPAPSPLTQGLATFTTQSYTFT
                     LADHGSHTFVGAVTFGKGGAEILQVTQANNHEVRGKATFAIS"
BASE COUNT         1121 a         1747 c         1751 g         1121 t
ORIGIN      
        1 acgtcggggt cgtcgatcac ttcagccgca tccgtagtaa gccagacgcc gtccaagcgg
       61 caggctcgcg gcttttccag gtcgcgcacg gccgcccagg cccagcggac cctgcggccg
      121 gcccggcggg cgatccggtc ggggtgctcg gtcaagaggc gcgccacgcc ggttcccaca
      181 gttcccagac cgatcaagcc gatggtgatg tgtttcattc gcgagcgaat caagcttgaa
      241 gacgcggaat caggctttcg gcccaggctt tatcttcctg gtcgagggga ggatcgggca
      301 agcccgagta aatgtaatcc tcgtagcccg atgcgtcgta gacgtgattg agcgcctctt
      361 gaaggtcgag tcgggcgtcg ctggcggggg cgcgaagtgg gaccggaatg accggcagcc
      421 gattacgcag gccgatcggc cagaatccgg ctatcggtct gtcatccact cggctcacga
      481 ggaccgaata ggcgtaggcg cgatcgggac gctcatccag cggcatgggc ttgccgccac
      541 gcagcaagtc gatctccacc aggttggctc ggctgtccag aagttcctgc cgcttggcca
      601 gatattgatc tcgactcgag cccgactgct tattggacgg gctgagcacc tcgataacgg
      661 tcacgagctc tcgactgcgg cgatcacgta tctccacgaa agccaactgt tcaacatcat
      721 gcacgggcaa acgcacttcg gtcggcgcac ccagcaggcc gattccagat cgtccggtaa
      781 tatcctcgga tcgggacacg gaaacatcgg ctcgtcccag ataacgtcga ggctccggcg
      841 gtagctcgtg aacgtatatg tgctcatcaa tcttgactat atagtctggc cgcacctgaa
      901 ctacaagtcg ctcggcgatc atggggatga tcttctcatg gaagtcatgc caggcgtcat
      961 cctgctccag gaaggggttc atgccgggaa agggcgatgg cattggtgga actccgggaa
     1021 tcgtcgtgac atccagtgtg tgtgtgtgac tcttgtggtt cgtacgaacg ttatgtaatg
     1081 accgccagcg gcccgcgcgt cacgccatat tggttcctcg ccttcagctc gcgccagaga
     1141 gccttaccct cgcgttcacc ggcgatcacc tcgcgataga gagtgatcgt gcgctcgacg
     1201 gttgcaggtg aatcatcgag aaactcgata cgcagaaagc gggcgccgtg cttttgcaac
     1261 cgcggcagat actcaacggc cgtctggggc accgcgttat agagcgtatt gcgacaaccg
     1321 acatctgcct tcaagggatg atccatgccg acgcgatcgc gcagctttac atcgtgggtg
     1381 tcgcaggggc ggccgcagtt ggtcgcgtcg gtacccggtg acaggaatgc acagtagaca
     1441 cagtgctcca tgtgaaacat gggaatttgc tgatgaatca ccacttcgat ccactcgggc
     1501 ggcgtcgagt cgatcaagtc catgagctgg tcgacattca ggtcgtaaga ggctgtcacc
     1561 cggacggccc cgcggctctt caagagctcg accgaaagcg gattggccgc gttgagcgag
     1621 aaatccgcca cgaacggaat accgcgctcg gaacaaaact tcatcccccc cgcattgcgt
     1681 gccaaaatgc catcggcccc ttgtttggcc agatacgaga agagattggc ctcgcccggc
     1741 ttctcgatgc gcggggtcgc caggtagatc gccgcccttg ccgtgccgcg gcggaccgca
     1801 gcgacggctt gggcgtactg tttaatgtcc tgatattcgg cgtaaatcgt cgaaacgcca
     1861 aatgtcacgg ccgcctcgat ttgctcggta ttgcgacaga ggaccgagag ctccaggggc
     1921 tggtaacggt tgcgcaacct ttccaactgg cggtcgcgct ctcgagcgat cggcccgagc
     1981 aaagtaggca agaccggctc gatagcgatc gcgcgcgctg gcaccgcgga cgccaattca
     2041 tccagccggg caatcagctc cctgcgcaac tggttgagca ggctcatcgg taccatcggg
     2101 cgcccctcga tcgtggcttc gatgtcgata agctcatatg aggtcccgcc cagacgtccc
     2161 aattgagcct ggaacaactc gaggtcggcg ggtcgcgatg cggccggacc aagcacatcg
     2221 gccgattgga ccgaagcccg cagacccaag ggcgccctac cgctcaatcg taagcgctca
     2281 cccgtcacgg caaagacctc gagcgtcagc tctatcttac gactcggcgg tccttcaaag
     2341 gatcgtcgca gccggcgggt caactcgggg tcgtcggtct tccagacgcg ctggccgatc
     2401 tcgagtgcgc gcagctcgat gtctccgcgc ccgaaccgca gctcgacatg tccctgctcg
     2461 ccgccgccct tgactcgtga gcccgcgttt ttcccggttc gatcgagcgc aatcacctcg
     2521 tagacgcggc ccccttgctc ggcgcggccc gccctttcgt cgccgtcgaa gaccagccca
     2581 tcccccggct tgacctgggc cgataccttc agacgaacac ctgagctgct caccgattcc
     2641 acgaccccca gcggaatacc ccgtttcttg gcataatcgc cgcgaaccaa aactttatgg
     2701 tcggtgccgt ccagaaaccc atgcgaaaac ccgcgcgaaa atgagagctg catttcctcg
     2761 acgtcgcggc gagtgaacgc caccggatgg cccgcccagg ccgcatcaat cgcttcgcgg
     2821 taatggcgcg taatgttcgc cacatactcg gccgacttga gccgcccctc tattttgagg
     2881 ctggcgactc ccaggtcgat caaacgcggg attagatcgt acgccgccag atcttggggg
     2941 ctcagcaggt actggatgtt gtcgaggtcg acaagacggc cgtcgcacac aatctggtaa
     3001 ggcatccggc aggcctgggc acattcaccg cgattggccg accgaccccc gagtgcctca
     3061 ctggtcaggc attgaccgga gtaggccacg caaagggcgc cgtgcacgaa gacctcgacc
     3121 gggaaatcga tcgcactgcg gatcttatcg atttcggcaa gcgcaagctc gcgagccagg
     3181 atcacgcgtg tgcaacccaa ctctcgtgcc agcgccaccc cttcctcgct cgtgatcgac
     3241 atctgagtcg aggcatgaat ctcgagatcg ggtgtgatcg ctcggatcag acgaacaagt
     3301 cccagatcct gaacgatcgc cgcgtcaaca cccgccgttg ccaggtcgcg tatcgtctct
     3361 tcgagactgg tcagctcgtc ggggaagaca agcgtattca gggtcacata gcctttcaca
     3421 cccctacggt gcaagagggc catgacctcg ggaaggtcgg ttccatcgaa attactggca
     3481 cggatgcgcg cattatgccg ctggagaccg aaataaacgg cgtctgcgcc attttcgacg
     3541 gcagcgatca agcacgtccg atcgccagcc ggtgcgagca gctcgggctt gacacgcgac
     3601 cgcaatccga gttgtcgaga cactccacca agcgcgtgcg taacagcgct gggctcggtg
     3661 accggctcgt gcgtctcgat cataaaactg ctttcaatcg ggatgttatc gcgcgttgga
     3721 actccaggcg acccaactga gtttagcggc tcgtactgaa atcggcatca ccttttcaga
     3781 ccaggacagt tcatcgtccg attgagaatc gacggcgatc gattcgaaac gccggtcccc
     3841 aaagcgagac gatcgatctt cctcgccggc ggaaaaccag ggtgacgacc aacaagtttt
     3901 ctggcgtgat accccacgta cgactcaact gatggcaaaa gtcgctttgc cgcgcacctc
     3961 gtggttgttg gcctgggtga cctgcaagat ctcggctccg cctttaccaa aagtgaccgc
     4021 gcctacgaat gtgtgtgatc cgtggtcggc gagtgtgaag gtatacgact gcgtggtgaa
     4081 agtcgccaga ccctgggtca gtggggacgg tgcgggggtc ttaaggctcc tgtacaccgc
     4141 tgtcgggaag gtcgtccagg aatcggtggg gctggacaat aacaccgtgc cggtgtaatt
     4201 cgtcacgggc tgcttctgtg catcgacggc cgtcaccgtg aagctcaagg gcacattaat
     4261 gtgggctctt cgcaatgtcg gggtcactcg gaacgaggcg ggcctcagcg gggtgactgt
     4321 gacgttgacg gggggcgagg cgctctcggc gaaacggttc gtgttcggca gatacttggc
     4381 ctcgatctga taggtgccga ccttttcgag cttggaagtc gtcaggctga gctgctggcc
     4441 gaacgtgccg acgtcgacct tgcctaacac gacggggtga cggcccaccg tgacaaactc
     4501 cacgactccg tcggcgaggc tgctgttggt attttgaacg ctggcgctaa aggtcatcgg
     4561 ctcgcccgtc tcgacactcg gcgtcacggc cgtgaggctc gtgacggtag gggcgttcac
     4621 cgttgtggga gtgatcgtca tggccgtgcg tgccgagttg ctgcccgcga aatagttatt
     4681 ggcagacacg aagtcggcct cgatctggta cggccctacg tttttcagca tgttcgtcga
     4741 gagggctgcc tgtgcgtttt tattcagagt aactttgccc agaacgacgg gttgaggaga
     4801 atcttcgacg aactccaccg ttccactgat gggttccacc ttgccagcgt tcacctggtc
     4861 gcctgtgccc gcatcgtgca cggtcgcgag caatggaacg gactgacccg cttccgcgct
     4921 tgcggccccg gatacgacgg tcgtggctgt cggtacgtcc agtggcagcg gaatgacatt
     4981 ggtagtctcg ggactcgaca cgctcgccga gacgctggag ttgctcggtg tgtactgcgc
     5041 ctcgatttgg taacttccga tcgtggtgag gtcgctggtc acaatggtag cctggccctg
     5101 gctgtttacg ctggcttctc cgagcacaat tttattgggc gactcggcga cgaaggtgac
     5161 atttcccgag gtgatcgggg cgccggtact ggcgttttgc actacgccga gaaaagccac
     5221 ttgtgcgccc gccacgacag tggggaaatt cgtgtagatg gtggtctgag aggatatgct
     5281 cgccgcggtc gtagctcccg tgccgctgag catggtccgc ccttccaggg cttccaggac
     5341 atagcgcgcg ggcaccgggc gacgacgcga gtcccgaccc caaaggcgcg caaagtacga
     5401 catcgtttcc tcgctggcag tcggcggtcg atcgacgtga cgctcacacg ggcctaagac
     5461 acgcggaaaa taccgaaagc ggtcgcgcag ccgacccttt caagacaaga tgggaagcga
     5521 catgttaagg agcgtctcga ttttgtcaag acctgtgagg ggttgcgcgc gacggcgggg
     5581 tgcgccaacg gattctggca ttcgctcgcg aaaactctag ccgcggtctg tgaccccggc
     5641 ccatcgcgaa ccgccggcct cacagaggcc agctacaaag accccggtcg cacgaatccg
     5701 ttggcgcacc cccgtgtacc aggcgagggc gttcgagatc
//
LOCUS       DATAQL010001410         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4267546, whole genome shotgun sequence.
ACCESSION   DATAQL010001410 DATAQL010000000
VERSION     DATAQL010001410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4267546"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2648)
                     /locus_tag="VHS97_24345"
     CDS             complement(<1..2648)
                     /locus_tag="VHS97_24345"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX3451406.1"
                     /translation="MLHVFSLIALTFPLLGQVPDKVDALQTVAERSLYRATARYDDVA
                     AWCREFAKVTPNAHLTELGRTSEGRSIPLLIVADPPITTAAQAASSGKLVCLVIGNIH
                     AGEVCGKEALPMLLRDVFGSRHPALLKDVILAVAPIYNTDGNERVSKTNRPGQVGPED
                     GMGQRANARGLDLNRDFIKLEAPETRGLVRFLNEWNPHLFIDTHTTDGSYHQYIVTYD
                     GPKNPAGDSRVEGFMRHTFFPAVTTAFEKRPGMKSFYYGNFNRDHTQWTSYPAEGRFG
                     TTYVGLRNRLSVLSEAYSHAPFKTRVLATRDFVLDCLQTAVNHKAEIVKLLAQARPSP
                     GAPGREAGESSETVSVPIRSRVKASDKPVTVLGYVERDENGRRAKTDTPKDYSLQLMN
                     EFEATESVSRPFAYLVPPSASEAIANLKRHGLDLQELREDIELDVEIYKIDAIDKSPR
                     RFEGHNMVELKTSVRRVAQKVPAGTLMVRTAQPLGALAVYLLEPRSEDGLAAWNFFDA
                     ELKAGGDFPVLRMIKSVPISIAAAEPLAETVGPLRPITFDTPGAAGGGRMRGGFMGAP
                     RWLDGEHWLAVREGRLMKVHAATGRTLPFIDTKALANGLSRLSSLDAETARSIAARTS
                     FDMDPGKQGFLFEQGQDLYYATFDGSKAVRLTTDPGREQFPQFSPDGKSVAFVRDFDL
                     YVVDIAGPTERRLTTGGRDDLRHGHADWVYYEEIFNRRWPAFWWSPDSKKLAFMEFDD
                     TGVPFHTVLDDTASPRREEKTHYPRSGEPNPKVRFGVVAGAGGPVQWADLSDYSADSF
                     LISDVGWWRDGSAAYCYVQNRTQTWLDFVKFTPDAEAPAVNRLFRDATKAWIESPGPV
                     HWLDDGTFLWLSERDGWKHIYHYDAGG"
BASE COUNT          538 a          899 c          908 g          536 t
ORIGIN      
        1 ccgccggcgt cgtaatggta gatatgtttc cagccgtcgc gctccgagag ccagaggaac
       61 gtgccgtcgt cgagccagtg caccggcccc gggctctcga tccaggcctt ggtcgcatcg
      121 cggaacaacc gattcaccgc cggcgcctcg gcgtcgggtg tgaacttgac gaagtcgagc
      181 caggtctggg tccggttctg gacgtagcaa taggctgccg aaccgtcgcg ccaccagcct
      241 acgtcgctga tcaagaatga gtcggccgaa tagtcggaga gatcggccca ctggaccggc
      301 ccgccggcac cggcgaccac gccgaatcgg accttggggt taggctcacc agatcgggga
      361 taatgcgtct tttcctcccg gcgcgggctg gccgtatcgt caaggacagt gtgaaacgga
      421 acgccggtat cgtcaaactc catgaatgcc agttttttgg agtcggggct ccaccagaaa
      481 gccggccagc ggcggttgaa gatctcctcg taataaaccc agtcggcgtg cccgtgccga
      541 aggtcgtcgc gcccgccggt agtgagcctg cgctccgtcg gaccggcgat gtcgacgacg
      601 tagaggtcga agtcacggac aaacgctacg gacttgccgt cggggctgaa ctgggggaac
      661 tgctcgcggc cgggatcggt cgtgaggcgc accgcttttg aaccgtcgaa ggtggcgtaa
      721 tagaggtcct gcccctgctc gaacaagaag ccctgcttcc cggggtccat gtcaaacgac
      781 gtccgtgcgg cgatcgaccg cgcagtctcc gcgtccagcg agctgagccg agaaaggccg
      841 ttggccagtg ctttcgtgtc gataaacggt agggtgcggc cggtcgccgc atgtaccttc
      901 atcagccggc cctccctgac ggccagccaa tgctcgccat caagccagcg cggcgcgccc
      961 atgaaacccc cccgcatccg gccgccgccg gccgcgccgg gagtatcgaa ggtaatcggc
     1021 cggagcgggc ccacagtttc cgcgagcggc tcggcagccg cgatcgagat cggcaccgac
     1081 ttgatcatcc ggagtaccgg gaaatcgccg ccggctttca actcggcgtc gaagaaattc
     1141 caggctgcca atccgtcttc cgaccggggc tcgagcaggt aaaccgccag ggcgccgagc
     1201 ggttgcgcgg tgcggaccat cagcgtgccc gcgggaacct tttgtgccac gcgcctcaca
     1261 ctggttttca gctcgaccat gttatgtccc tcgaaccggc gtggcgactt atcgatcgcg
     1321 tcgatcttgt agatctcgac gtcgagctcg atgtcctcgc gcagctcttg gagatccagg
     1381 ccatgccgtt tgaggttcgc gatcgcctcg cttgcggagg ggggaaccaa gtaggcgaac
     1441 ggccgcgaga cagactccgt ggcctcgaat tcgttcatca attgcagcga atagtctttg
     1501 ggcgtgtcgg tcttggcccg gcggccattc tcgtcgcgct cgacataccc gagtacggtg
     1561 acgggtttgt cggacgcctt gacgcgcgag cggatcggta ccgagaccgt ttcgctgctc
     1621 tcgccggctt cacgtcctgg cgctcccggc gacggccgcg cctgcgcgag cagcttgacg
     1681 atctcggcct tgtggttcac ggccgtttgc aggcagtcga gcacgaaatc gcgagttgcg
     1741 agcacgcgcg tcttaaaggg agcatgggaa tacgcctccg agaggaccga aagccgattt
     1801 ctcagtccca cgtaggtggt gccaaaccgc ccctcggctg gatacgacgt ccattgcgtg
     1861 tggtcgcgat tgaaattgcc gtagtagaac gacttcatgc ccggccgctt ttcaaaggct
     1921 gtggtgacgg cgggaaaaaa cgtgtgtcgc atgaagcctt cgaccctgga atcgccggcg
     1981 gggtttttgg ggccatcgta cgtgacgata tattggtggt aggatccgtc ggtcgtgtgg
     2041 gtatctatga acaggtgcgg attccactcg ttcaaaaacc gcaccaaacc acgcgtctcg
     2101 ggcgcctcca gcttgatgaa gtcgcgattg aggtcgagcc cacgggcgtt ggcgcgctga
     2161 cccatcccgt cttcggggcc gacctgtccg ggccggttgg tcttggaaac ccgttcattg
     2221 ccgtcggtgt tgtaaatcgg cgcgacggcg agtatcacgt ccttgagcag agcgggatga
     2281 cggctgccga acacgtcccg gagcaacatc ggcagcgctt ccttgccgca aacctcgcct
     2341 gcgtggatgt tgccgatgac gagacagacg agcttgcccg agctggcggc ctgggcggct
     2401 gtggtgatcg gcggatcggc tacaatcaac aggggaatcg accgtccttc cgacgtccgg
     2461 ccgagctcgg tcagatgtgc gttcggggtg accttggcaa actcacggca ccatgcggcc
     2521 acgtcgtcgt accgagcggt agcgcggtaa aggctgcgct cagccactgt ttgcaacgcg
     2581 tcgaccttgt caggaacctg acccagtaaa ggaaacgtta aagcgatcaa gctgaaaacg
     2641 tgaagcatca ccggaagtcc ctcgcgcacc gaaaccaggg tttcacaacc aaagcatcat
     2701 ttgccagacc acgtcgattt tcccggtacc aagctatccg accgcgaccg ccaaatcgag
     2761 gcctgccaac cttatcggcc tttttctaag gtctcgtagg atgagcatcg ccccccaggc
     2821 acccccagcg ggaaaacccg gtcgagctgc cacgacagcc gtactccgcg atcgacgagg
     2881 t
//
LOCUS       DATAQL010001411         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4113587, whole genome shotgun sequence.
ACCESSION   DATAQL010001411 DATAQL010000000
VERSION     DATAQL010001411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4113587"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VHS97_24350"
     CDS             <1..450
                     /locus_tag="VHS97_24350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451407.1"
                     /translation="EKMRGVRDELQGLEADARRTKMLEINKEMNASALKAFGEFLKPD
                     QITRLKQISYQVRGPMVFSDPEVASKLNITDSQKSEIQTIVEESRGKMPTREDFTDNR
                     EDAMKKAAEVQKATLAQVTAKLNDEQQKTWKELVGAPFEIKYEPRPQ"
     gene            complement(602..2443)
                     /gene="glmS"
                     /locus_tag="VHS97_24355"
     CDS             complement(602..2443)
                     /gene="glmS"
                     /locus_tag="VHS97_24355"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX3451408.1"
                     /translation="MCGIVGYTGAHEAAPILVAGLRRLEYRGYDSAGLATLDGNEMNL
                     RKRAGRVRALEELLDCEAAPGACGISHTRWATHGPATDKNAHPHLGGRGGRAAVAVVH
                     NGVIENHAALRRELESQGFNFVSQTDTETIAHLIARELEDGNDLFEAVQRALPRLQGT
                     YGLAVVSPGRPGEIVGARFGSPLVVGLGEGEHLLASDAVAIAPHTLKVAYLQDGEVVR
                     LTDREFEIRHLEHGPITPRIDRIDWKPDTVELGGHAHYMIKEIRAQPETVVDACRGRL
                     RRAEATAHLGGLNLTARQLRRVRRIVFAACGTSWHAALVGEYLVERLAHLPVEVEYAS
                     EFRYRNAPLDDRTLVFVLSQSGETADTLGALREAKRRGHPTLAIVNTVGSTIAREADG
                     GIYLHAGPEVGVASTKAFSAQVTVLLLLALYLGRLRQLSFEDGLSVVDAIESVPDLLE
                     DTFNAEPAIEAAAERVSSAKSALYLGRDLHFPVALEGALKLKEISYIHAEGYPTAEMK
                     HGPIALVDSVTPCIFVAPRGALHAKTMSNLEEVKARNGTIITVGTVGDRALVELSDVF
                     LPIPEAPEIVQPLLAVVPLQLLAYHVARLRGCDIDKPRNLAKSVTVE"
     gene            2993..3922
                     /locus_tag="VHS97_24360"
     CDS             2993..3922
                     /locus_tag="VHS97_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3451409.1"
                     /translation="MRILVTGGAGYVGSTMVPMLLEQGHRVRVVDTLKFGGHGLLPCC
                     ANRFFELTKGDICDPATAKAALDGVEAVIHLAAIVGYPACKKEPQLAQTVNVDGTANL
                     LRHRKPDQKFLFASTGSIYGSIPDYVCNEETPRAPITLYGETKAAAEQSVLDAGNSVA
                     YRFATAFGVSNRMRLDLMPNDFTYQAVHNRNLIVYEGGFKRTFVHVRDMARSFIFALE
                     RWNEVKDDVYNVGHESMNFTKEEVARKILEHVDYYLHFAEVGSDADQRNYEVSYEKIR
                     KIGFETTVDLDRGVAELVRAARLIEFQNPFANV"
     gene            3961..>4272
                     /locus_tag="VHS97_24365"
     CDS             3961..>4272
                     /locus_tag="VHS97_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3451410.1"
                     /translation="MKSFWSGKRINVTGGAGFLGQHVVKRLESYGATVFVPRQREYNL
                     VSLDACLRCMLEHPCDVLFHTAAYYGGIGINQSEPAKLYYTNLVMGANLMEAARLSQV
                     AK"
BASE COUNT          860 a         1407 c         1252 g          753 t
ORIGIN      
        1 gaaaagatgc gtggagtccg cgacgagttg caggggctcg aggccgatgc gcggcggacc
       61 aagatgctag agatcaacaa ggagatgaac gcgtcggctt tgaaagcatt cggtgaattc
      121 ctcaaaccgg accagatcac acgcttgaag cagatttctt atcaggtgcg gggccccatg
      181 gttttcagcg acccggaggt ggcctcgaag ctcaatatca ccgactccca gaaaagcgag
      241 atccaaacga tcgttgaaga gtctcgcggg aagatgccca cccgcgaaga tttcaccgat
      301 aaccgcgagg atgcgatgaa aaaggcggcc gaagtgcaga aagcaaccct cgctcaagtc
      361 accgccaagc tcaacgacga acagcagaag acctggaaag agctggttgg cgctccgttc
      421 gaaatcaaat acgagccgcg cccgcagtga tccagtttgg cgaccgttgg cctaggagca
      481 gtgaaggcgc tcggaagtga cccgccgccg acacgcaacg agatctcgga tccttcggtc
      541 cgaggtctcg ttttgctttc tcgccggcgg gcggttcaga tacggtcgag ccgggccggt
      601 gtcactccac ggtgacgctc ttggccagat tgcgcggttt atcgatatca cagccgcgca
      661 gccgcgccac gtggtaagcg agcaactgca gcggcacgac ggccaggagc ggctgcacga
      721 tctcgggcgc ctcgggaatc ggcaagaaaa catccgatag ctccaccagc gcgcgatccc
      781 cgaccgtgcc gaccgtgatg atcgtgccgt tgcgtgcttt gacctcctcg agattgctca
      841 tggtcttggc gtgcaacgcg cctcggggag ccacgaaaat acagggcgta acgctatcca
      901 ccagcgcgat cggcccgtgt ttcatctcgg ccgtcgggta cccttcggca tggatgtagc
      961 tgatctcctt gagcttgagg gccccttcga gcgccaccgg aaagtgcagg tcgcgaccga
     1021 ggtagagggc gctcttggcc gagctcacgc gctcggccgc ggcttcgatc gcgggctctg
     1081 cattgaaggt atcttcgagg agatcgggaa ccgattcgat tgcgtccacc acggacaggc
     1141 catcctcgaa cgaaagctgt cgcaaccggc ccagataaag ggccagaaga agcagcacgg
     1201 tcacctgcgc cgaaaaagcc ttggtgctgg cgacgcctac ctccggcccg gcatgcagat
     1261 agatgccgcc gtcggcctcg cgggcgatcg tgctgccgac ggtgttgacg atcgccaacg
     1321 tcgggtgccc gcggcgcttt gcctcccgca aggcacccag ggtgtctgcc gtctcgcccg
     1381 actgggagag cacaaagacc agcgtgcggt cgtcgagcgg tgcattgcgg taacggaact
     1441 cgctggcgta ttcgacctcg acgggcaaat gagcgagccg ctcgaccagg tactcgccca
     1501 ccaaggccgc gtgccagctc gtcccgcacg ccgcgaaaac gatccgccgc acccggcgaa
     1561 gctggcgggc cgtcaggttc aagccgccga ggtgcgcggt cgcttcagcc cgtcgcagcc
     1621 ggccgcggca cgcgtcgacg accgtctcgg gctgcgcacg gatctccttg atcatgtaat
     1681 gcgcgtgccc ccccagctcg accgtgtcgg gcttccagtc gatccggtcg attcgagggg
     1741 taatcggtcc gtgctcgagg tgacggatct cgaattcacg atcggtcagg cgaacaacct
     1801 cgccatcctg caaatacgcg accttgagcg tatgaggcgc gatcgccacc gcgtcgctgg
     1861 ccagcaggtg ctccccctcg cccagaccga cgaccagcgg actgccaaac cgcgccccca
     1921 caatctcgcc aggccggccg ggactgacaa ccgccagccc gtacgtccct tggagtcgag
     1981 gcaaagcccg ctgcaccgcc tcgaacaggt cattcccgtc ttcgagctcg cgcgcgatca
     2041 agtgcgcgat cgtctcggtg tcggtctgac tgacaaaatt gaatccttgt gactcgagct
     2101 cgcgccgcag ggccgcatgg ttttcgatga cgccgttgtg aacaaccgca acggccgccc
     2161 taccccctcg ccccccgaga tgcggatggg cgttcttgtc ggtcgccggt ccgtgagtcg
     2221 cccagcgcgt atggctgatc ccgcaagccc ctggggcggc ctcgcagtca agcaactctt
     2281 ccagagcgcg cacgcggccg gcccgcttgc gcaaattcat ttcattccca tcgagcgtcg
     2341 ccagacccgc gctgtcgtag ccgcgatatt cgagccgccg cagcccggcg accaggatcg
     2401 gcgcggcctc gtgggctccg gtgtaaccga cgattccgca catcgccacg gtccttccgc
     2461 gaaagacttg ctctcagccg aattcgggtt tccccgcggt ccgctcggga aaaatgccgc
     2521 cactttgctt cggaagcccg gcagcacttc ctcacccgca agattcgaac gaaattataa
     2581 ctcgggagcc ctttttgcgg atatcccgcg atgccatcct gcaaaaaagg cccagaaaaa
     2641 agaggcccag cccgtcgccg atcacgccct aaactcgatc ctatctcaga gcgccaatgg
     2701 cgatggggaa aaggagtggc tcgttcccca aaacatcgcc aaagtggcgt tgacaggccg
     2761 tctggactgg gcgatagtgt cgccccgacg ggacggcttc gggcgaggcc ggccggcttc
     2821 tgcggaaagc aatgacgatc gcctgtcacg cacggcaaca ctacggaaac ggccgcaagg
     2881 acgagggcct ggcgacttct tctccccgtc tctccttgcc gcatggttta cccttaaaca
     2941 gacaccctcg acgtaccagg cgcgtttcga gccaccaagg atggagcccg cgatgcgaat
     3001 tctcgttacc ggcggagccg gttacgttgg ctcgaccatg gttcccatgc tcctcgagca
     3061 gggacaccga gttcgagttg tcgacaccct caagtttggc ggccacggcc tcttgccctg
     3121 ctgcgccaat cgctttttcg agctgaccaa aggcgatatc tgtgatcccg cgaccgccaa
     3181 ggcggcgctc gatggtgtcg aggccgttat tcacctggcc gccatcgtgg gataccccgc
     3241 ctgcaagaaa gagccccagc tcgcccagac ggtgaacgtc gacggcactg ccaacctgct
     3301 ccgccaccgt aagcccgatc agaaattcct cttcgcatcc accggcagca tctacggctc
     3361 gattcccgac tacgtctgca atgaggaaac cccccgcgcc cccatcaccc tctacggcga
     3421 aacaaaggcc gccgccgagc agtcggtgct cgacgccggc aacagcgtcg cctaccggtt
     3481 cgccactgca ttcggcgtca gcaatcggat gcgcctcgac ttgatgccta acgacttcac
     3541 ctatcaagcc gttcacaacc gcaacctgat cgtctacgag ggaggcttca agcgaacgtt
     3601 cgtccacgtg cgcgatatgg cccggtcatt tatcttcgcg ctcgagcgct ggaacgaggt
     3661 caaggacgac gtttacaacg tcggccatga gtccatgaac ttcaccaagg aagaggtcgc
     3721 gcgcaagatc ctcgagcacg tcgactatta tctccacttc gccgaggtcg gatccgacgc
     3781 cgatcagcgc aactacgaag tctcgtacga gaagatccgc aagatcggct tcgaaaccac
     3841 cgtcgacctc gaccgcggcg tcgccgaact ggttcgtgcg gccaggctga tcgagtttca
     3901 gaacccattc gccaacgttt gatcgatgac caatgaccaa tgacagcagg agcatcaacc
     3961 atgaagagtt tctggtccgg caaacgcatc aacgtcacag gcggagccgg cttcctcggc
     4021 cagcacgtgg tcaagcggct cgaaagttac ggcgcgaccg tgtttgtgcc tcgccagcgc
     4081 gaatacaatc tggtctcgct cgacgcgtgt ctgcggtgca tgctcgagca tccgtgcgat
     4141 gtcttgtttc acacggccgc gtattacggc ggcatcggca tcaatcagag cgagccggcc
     4201 aagctctact acaccaatct tgtcatgggc gctaacctga tggaggccgc gcgactttct
     4261 caggtcgcca ag
//
LOCUS       DATAQL010001412         6184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_450023, whole genome shotgun sequence.
ACCESSION   DATAQL010001412 DATAQL010000000
VERSION     DATAQL010001412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450023"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VHS97_24370"
     CDS             complement(<1..456)
                     /locus_tag="VHS97_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3451411.1"
                     /translation="MSGPAPASRRTTSELAGQVALITGAAHGQGRASALALAREGVRI
                     AALDVARPMTYPGYPMGSADDLETLSQDCRALGVDCLTFAVDVRDDAGVGIAVAATID
                     QFGSIDILFNNAGIRGYGLAHELTEDAWDSMLDINLKGPFLVGRRVIPIM"
     gene            complement(456..986)
                     /locus_tag="VHS97_24375"
     CDS             complement(456..986)
                     /locus_tag="VHS97_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451412.1"
                     /translation="MARISPLQDEGAHPESRAALDQIRTAHGRVTNMKRTLARSPVAL
                     RALMTWYDLRDEVVPFLGERPTTLFAHAISAGTDCLVCSTFFRRLLVDAGEDPEALRL
                     DEWEQTVVAYGRQLAVDPHGVSDELYGRLAARLEPDQIVVLTAFGGLMVATNLFNNAL
                     QVDLDDYLQPYRKEKR"
     gene            1407..1907
                     /locus_tag="VHS97_24380"
     CDS             1407..1907
                     /locus_tag="VHS97_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3451413.1"
                     /translation="MTYAETILPEFDQEMANTRKVLERIPDDRLDWQPHPKSHTIGWN
                     ANHVADLPNWLVETLTKPSLDIAPVGGEPYKFPKASSRQEILDVFDRNVAAARKAITQ
                     VTDEDATSMWTLSQGGKPIFTMPRSAVVRGMVLSHLIHHRAHLCVYLRLNNLPVPGMY
                     GPSGDE"
     gene            2657..3448
                     /locus_tag="VHS97_24385"
     CDS             2657..3448
                     /locus_tag="VHS97_24385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451414.1"
                     /translation="MRRTKTAVLPSVDVMEPRLLLSTAAPLLSKHALTGVVREIRAIV
                     STLAKTGDTAQASAQLAGLSSQIPSGPAGLAQSWQSDIGLYRPHSARSIVSMETRIVG
                     DLDRYNRGVVDGGNRPVSGSGSTTSTTPNQGTGGTATPVPMPKSGQGTAGTSTPAPLP
                     SLDSVRIQNTTGLALQVTVHLEVPQNHQPSITETIPAQGSSIASFDFGTATNVFMTMD
                     VSRADGGGQSPPPWTNINLSQPLSGYNGVLFTISLFGSYFSVNVP"
     gene            complement(3508..4140)
                     /locus_tag="VHS97_24390"
     CDS             complement(3508..4140)
                     /locus_tag="VHS97_24390"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX3451415.1"
                     /translation="MTESQDKRLIVGVTGATGIVYAARALKVLRSIGIETHLIVSRAG
                     QVTRAYETDLSKADLASLADHVYAIMDMGAPVSSGSFKTLGMLIAPASMRSVAEIATG
                     VTSSLLTRAADVVLKERRRLVLMVREAPLNLIHLRNMVTITEAGGIIFPPVPAFYSRP
                     TTLEQVIDQSVGRALDQFGLHTDIFPRWDEDLRKKVAKHETVISTRGSSQ"
     gene            4732..5544
                     /locus_tag="VHS97_24395"
     CDS             4732..5544
                     /locus_tag="VHS97_24395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451416.1"
                     /translation="MAQAPTIDLAAAGGLGATERRDGWWIGPLATGLGLGGFIIYATF
                     RACYNADYRLGAGTGVLEGHAYLLSPLYSPLIVLPASLAWISPALFILWAPGGFRVTC
                     YYYRKAYYRAFFLDPVGCAVGEPSRFCGIRRGREYQGESRLLLFQNLHRYFLYLALVF
                     LVILGLDVVHSCRWPDGFGVSVGTLVLAVNTAMLTLYTASCHSFRHLIGGRLDGFSRV
                     GLGKLRYRWWSWASALNKHHMLWAWVSLFSVAFADFYVWMVASARITDIRLI"
     gene            5549..>6184
                     /locus_tag="VHS97_24400"
     CDS             5549..>6184
                     /locus_tag="VHS97_24400"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX3451417.1"
                     /translation="MDKYEQQEYDVLVVGAGGAGLRAAIEASSLGARTALVCKSLLGK
                     AHTVMAEGGVAAALASADTRDSWQVHFRDTMRGGKMLNQWRMAQLHAQESPARVRELQ
                     EWGAVFDRTREGDILQRNFGGHTYPRLAHVGDRTGLEMIRTLQDRGVHQGIDVFMECT
                     LRALLKEGDHIAGGFGYLRESGRFVLFRAKAVVLATGGIGRCWEISSNSWEY"
BASE COUNT         1194 a         1862 c         1899 g         1229 t
ORIGIN      
        1 catgatgggg ataacccgcc gaccaacgag aaaaggcccc ttcagattga tgtcgagcat
       61 ggaatcccag gcgtcttccg tgagctcgtg cgccaggccg taaccgcgga tccccgcgtt
      121 gttaaagagg atgtcgatgc tgccgaactg atcgatcgtc gcggcaacgg ctataccgac
      181 accggcatcg tcacgaacgt cgacggcgaa cgtcaggcag tcgacaccga gcgcacgaca
      241 atcctgagag agggtctcca gatcgtcggc cgagcccatc gggtaacccg gataagtcat
      301 tggccgcgcg acatcaaggg ccgcgatccg gactccttcg cgagccagcg ccagcgccga
      361 tgcccgccct tggccgtgcg cggctcccgt aataagggcg acctggccgg cgagttcgga
      421 cgtcgttcgc cggctcgccg gcgccggtcc gctcatcacc gcttctcctt gcgatacggt
      481 tgcaggtagt catcgaggtc gacctgcaat gcattgttga aaaggttggt cgcgaccatg
      541 agcccgccga atgcggtcaa tacgacaatc tggtcgggct cgagccgagc ggccagacga
      601 ccatagagct catcagacac gccatgcggg tcaacagcga gctgccggcc gtaggcgaca
      661 actgtctgct cccattcatc gagccgcagg gcctcggggt cttcacccgc atcgacgagc
      721 agccggcgaa agaaggtcga gcagacaagg cagtcggtcc ccgccgagat ggcgtgggca
      781 aagagcgtgg tcggccgctc gcccagaaat ggaacgacct cgtcgcggag gtcataccag
      841 gtcatgagcg cgcgcagggc gaccggcgag cgcgccaacg tccgcttcat gttggtcact
      901 cgcccgtgcg ccgtgcggat ctggtcgagc gctgcgcggg attcggggtg ggcaccttcg
      961 tcttgcagcg gtgaaattcg cgccatagtc cggcctgata gcagccttca aattcggtta
     1021 gtcgaccttg ccggtgacgt tctcgtcaag gttgcgcatg aattgatcgt agcatcttgt
     1081 gcgatctatg gtaattccag tcaggctcag gccagaatcg cgaccaggaa ggcgggaacg
     1141 gtgaattcta tctcgaccag ctcgtccgtc gcgagtacga gttcgcccag ctctggatca
     1201 cttgggccaa gccagtggta aagactgccc agaaagcgtg cttgttatcc ctattcgacg
     1261 aagggcctcc aaagtcgagg ctcgattccg aatctacctc ttgaaattag tgtacgggcg
     1321 ttgacaggcg gtcgataatt ggttccaatc cacgagggtc gacggcagta cccgaggtga
     1381 ggtcttaaaa ccgaatggag aatcccatga cttacgccga aactatcctg cctgagttcg
     1441 atcaagagat ggcgaacacc cgcaaagtgc tcgaacggat tccggacgac cggctcgatt
     1501 ggcagccgca tcccaagtca cacacgatcg gctggaacgc caaccacgtg gcggacctcc
     1561 cgaattggct cgtggagacg ctcacgaagc catcactcga tatcgctcca gtcggcggcg
     1621 agccttacaa gtttcccaag gcgagtagcc gacaagagat cctcgacgtc ttcgatagga
     1681 atgtcgcggc ggcgcgaaaa gcgatcacgc aggtcacgga cgaggacgcg accagcatgt
     1741 ggacgctgtc tcagggcggg aagccgattt tcaccatgcc ccgttcggcg gtggtgcggg
     1801 gaatggtcct cagccacttg atccaccatc gcgcccacct atgcgtctac ctccgattaa
     1861 acaacctccc ggttcctggg atgtatgggc catcgggaga cgaataaaca acagggatat
     1921 ggtgtccccg ccggccctcc ctgaggattc cgccggcgcg ggcacaccac ttgtgcgccg
     1981 taaaccgaga tccggaccat gttgtctgca gtacttgcaa tatggtcgag atgtgcgggg
     2041 cggaagttga cagcttcatc agggatagtt cggcaggatc ggaacaggaa aaagaactct
     2101 tgcaattgat gcacatatat ttacttttat ataatcagca gagaagcatt ttttgtgcaa
     2161 tatggtatac atcgcgcgct acgtcagcgc gaactcaacc gcacgctacc gctgagacag
     2221 tttgaacaat cgcgctggca gacattcgac tgacgatcat cgttctctgg cctggcgtcg
     2281 atcatcattc cctgctggaa caattgggtt tacacggcgg tgcatcgcag caatcgttcg
     2341 tcatacacgc cggccgtggg aactgatcgc ccatgtccgt ccttcggcgc aatgatgaac
     2401 ccgctttcgt ccgctctgcg ttccgtagcg tgaacgcccc gtggatccgc gacactatcg
     2461 acggtatccg ccgctcaagg atttaggcgc cgcgaaataa taaggtgagc gaatttcggc
     2521 tccgcagtgg cacgggcatc ttgcccgtgt ctaaaaatgc cacgggcaag atgcccgtgc
     2581 cacccagcct caccgattgc cagccgatgt ccgctccgtg tggtatagat attgtacgca
     2641 cctggaggat gaatccatga gaagaacgaa gacggccgtt ttgccgagcg tggatgtcat
     2701 ggagccgagg ttgttgctgt ccacggcagc acccctcctg tccaaacacg cgttgaccgg
     2761 cgtcgttcgc gaaatcaggg ccatcgtgag cacgcttgcc aagacgggag acactgccca
     2821 ggcaagtgcc cagctcgccg gactttcgtc gcagattcca tccggacccg cggggctcgc
     2881 gcagtcgtgg cagagcgaca tcggcctcta tcggcctcat tccgcgaggt cgatcgttag
     2941 tatggagaca cggatcgtcg gcgacctcga ccgttacaac cggggcgttg tcgatggcgg
     3001 taaccgacca gtatccggct cgggctccac gacctccacc actcccaacc agggcacggg
     3061 gggtactgcc actcccgtgc cgatgcccaa gtccggccag ggcacggcgg gtacgtccac
     3121 tcctgctccc ctgccaagtc tcgacagcgt gaggattcag aacacgacgg gattagccct
     3181 ccaagtaacc gtccatctgg aggtgccgca gaaccaccaa ccgtcgatca ccgagacgat
     3241 tccggcgcag gggagttcga tcgcttcctt cgatttcgga accgcaacca atgtcttcat
     3301 gacgatggac gtgagcaggg cggacggggg cggccaaagt ccgcccccgt ggaccaacat
     3361 caacctttcg cagccgctca gtggctacaa cggcgttctg ttcacgatct ccttattcgg
     3421 ttcttatttc agcgtcaacg tcccctgacc cccggctaat tgcctgaaca ttcgcgtggg
     3481 aatgccgggc gatcctcgaa gaggaactca ttgagaactg cctcgggtgc tgatcactgt
     3541 ttcgtgcttg gcgactttct tcctgagatc ttcgtcccat cgtgggaata tgtcggtatg
     3601 gaggccgaac tggtcgaggg ctcgcccgac tgattgatcg atcacctgct ccaacgttgt
     3661 gggcctcgaa tagaacgcag gtaccggtgg aaagatgatc ccaccggcct ccgtaatcgt
     3721 caccatgttg cgcagatgaa tcaggttcaa tggagcctca cgaaccatca gaactaatcg
     3781 gcggcgctcc ttgagcacca cgtcggcggc gcgtgtcagg agcgatgatg tcacgcccgt
     3841 ggcgatctcg gcaaccgaac gcattgatgc gggagcgatg agcattccca gcgtcttgaa
     3901 agaaccggat gacacggggg ctcccatgtc catgatcgcg tagacgtggt cggccagcga
     3961 cgcgaggtcc gccttgctga ggtccgtttc gtaggcacgc gtcacttgtc ctgcacggct
     4021 gacaatcagg tgcgtctcga tgccgattga gcgaagcact ttgagcgccc gtgcggcata
     4081 gacaatgcca gtggcgccag tgacgccgac gatgagtctt ttgtcctgcg attcggtcaa
     4141 gatgcgggct gctccagatt gagttgcggg gcatgagcac gaggattgac atcacagaat
     4201 tgcgcgcgcc gattcagacg ttccgattcc attgggctcc tgcctcattg atgggacgca
     4261 ggccttccca ttgcctcggc ttcgacattt tgaactgaat cagacccgcg ccgcagtctc
     4321 tctgtggtgg tgcccattgt caagaaccaa gatcggcgat tcttgagctt cagcaaggtt
     4381 cggcgtcgcc gaatcaaggt gtcgtggtaa tagcggacga ggggcgtgct gccccccacg
     4441 atactcagaa aagatcacca tcacactcgc ttggccatca agtcggcgag accgactgtc
     4501 gttgcagcgt gtgtcctttt ttcctttttt cctggacacg gtcgagtgga gcgggttgaa
     4561 tggcgcatca ggtgacctgg atggaggctg aacttcgctc tccacacttg ggagagttgg
     4621 tgcgacctga cccgtccggt cggtccggaa ctagggcgag tcggggccga attccaggaa
     4681 agcctggggc gattcatcgg ctcgacgcac ttcatagccg agggggtcac catggcgcag
     4741 gctccaacga tcgatctggc ggcggccgga ggattgggcg cgacggagcg cagagacggg
     4801 tggtggatcg gtcccctggc gacgggattg ggactgggcg gcttcatcat ctacgccaca
     4861 ttccgcgcgt gctacaacgc cgattaccga ctcggagcgg ggacgggggt cctggaaggt
     4921 catgcttatc tgctgtcccc cttatattcg ccgctgatcg tgctgcccgc atcgctggca
     4981 tggatctcgc ccgcgctctt catcctgtgg gcacccggcg gcttccgggt cacctgctac
     5041 tattatcgca aggcgtacta cagggccttc ttcctcgacc ccgtcggctg cgccgtgggc
     5101 gaaccgtcgc ggttctgtgg catccggcgc gggcgggagt atcaggggga gtcgaggctt
     5161 ttgctgttcc agaatctgca tcgctacttc ctgtacctgg cgctggtctt tctggtcatc
     5221 cttggcctcg acgtggtcca ttcttgccgc tggcccgacg gtttcggtgt gagcgtcggc
     5281 acgctggtcc tggccgtcaa caccgcgatg ctgactcttt acaccgcctc gtgccactcg
     5341 tttcggcacc tgatcggggg gcgactcgac ggcttctccc gcgtcggctt ggggaagctc
     5401 cgttaccgct ggtggtcgtg ggcctcggcc ctgaacaagc accacatgct ttgggcctgg
     5461 gtcagcctgt tctcggtcgc attcgccgac ttttacgtct ggatggtcgc gtcggcccgg
     5521 atcacggaca ttagattaat ctaagaacat ggataagtac gagcagcaag agtacgacgt
     5581 cttggtcgtg ggcgcggggg gggcggggct gcgggctgcg atcgaggcct cctcgctggg
     5641 ggcgaggacg gcgctcgtat gcaagtcgct gttgggcaag gcccatactg tcatggccga
     5701 gggcggggtc gcggccgccc tggccagcgc cgacacgcgg gatagctggc aggtccactt
     5761 ccgcgacacg atgcgcgggg gcaagatgct caaccaatgg cgcatggccc agttgcacgc
     5821 ccaagagtcg cccgcgcggg tgcgcgagct gcaggaatgg ggggcggtct tcgaccgcac
     5881 gcgggagggt gatatcctcc aacgcaactt cggcggtcac acctacccac ggctggccca
     5941 cgtcggcgac cgcaccgggc tcgagatgat ccgcacgctc caggaccgcg gtgtccacca
     6001 aggtatcgac gtcttcatgg aatgcaccct ccgcgcgctc ctcaaggaag gcgaccacat
     6061 cgccggcggc ttcggctatt tgcgagagtc gggacggttc gtcctgttcc gggccaaggc
     6121 ggtggttctc gccacggggg ggatcggccg ctgctgggag atcagctcga attcctggga
     6181 atac
//
LOCUS       DATAQL010001413         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1826245, whole genome shotgun sequence.
ACCESSION   DATAQL010001413 DATAQL010000000
VERSION     DATAQL010001413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826245"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VHS97_24405"
     CDS             <1..777
                     /locus_tag="VHS97_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HEX3451418.1"
                     /translation="TVRLLVVTGGPAVARAALASKRRAIVAGPGNPPVVVDATACLEN
                     AAKSIISGGAFDNNLLCIGEKQVFAVAQVFDQLMEAMTRHGGFRLTASQVDALTQAAF
                     QRGSDGALHVNKDLIGQDPAVLGRHAGVQVPQGTELLFGETGAEHAFVDHEQMMPFVP
                     FVRVSNVDRAIVLAKESEHGFGHTAVLHSRDTSVMARMGKIMDCTIFVVNGPSTAGLG
                     MTGEKVLSFSIAGPTGEGVTTPLTFCRQRRTTIAGSMRFI"
     gene            788..1048
                     /locus_tag="VHS97_24410"
     CDS             788..1048
                     /locus_tag="VHS97_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX3451419.1"
                     /translation="MQLGRVVGTAIATVKHPTLQAERMLVVQLETAAGQPDGEPILAF
                     DRLGAGRGDRVLVTNDGRALQELLGATTPGRWSVMGLPDDRS"
     gene            1082..1345
                     /locus_tag="VHS97_24415"
     CDS             1082..1345
                     /locus_tag="VHS97_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX3451420.1"
                     /translation="MIGRVTLSRSNPQLKGGRLLIALPMPLAALTEADPARGEEVVVY
                     DILGAGPGSLIALSEGREAANPFGKTKTPVDAYCACLIDELSF"
     gene            1403..2398
                     /locus_tag="VHS97_24420"
     CDS             1403..2398
                     /locus_tag="VHS97_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3451421.1"
                     /translation="MIGNGTVMNEWKFREQMCEIGRRIYNKGFAAANDGNISLRLGDD
                     RVLCSPTRVSKGFMKPDDLCIVDLDGKQISGKRKRSSEILLHLSIMKARADVRSVVHC
                     HPPHATAFAVAREPIPKCVLPEIEVFLGEVAISPYETPGGQSFADTVLPYVKDTETIL
                     LANHGTVTYGTDLEDAYFKTEIIDAYCRILILAKQIGRVNYYTDEKAAELIKLKPKLG
                     IPDPRLERGLENCDLCGNSLFREGYGGANPEPHVFIHPKIAGSEPVTSPIAGVLTPLY
                     SDLGKSAAVQASPSRVASASKPSNNGHTGAGSLDPDALVQAITDQVMAALNGALK"
     gene            2503..>3099
                     /locus_tag="VHS97_24425"
     CDS             2503..>3099
                     /locus_tag="VHS97_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEX3451422.1"
                     /translation="MKVSIIGGGGLVGSSAAFALQCGAVVSQIDLIDLNADQVRGQAL
                     DLLHGASLVADQRIRATGYEAIPESDVIIITAGLRRKPDESRLDLINRNVELFLGILG
                     QATAAGIKRDAIVLVVSNPVDVLTYLAVRLLDLPSSQVIGLGTQLDTARFRSLIAEKL
                     QLPPTQVTALILGEHGDSMVPIWSTAQAAGLPLERFAGW"
BASE COUNT          584 a          984 c          971 g          560 t
ORIGIN      
        1 actgtccgtc tgctggttgt gaccggcggg ccagcggttg ctcgtgccgc cctggccagc
       61 aagcggcggg caatcgtcgc tgggccgggt aatccgccag tcgttgtcga cgccactgcc
      121 tgcttggaaa acgcggccaa gtcgatcatc agcggcggcg ccttcgacaa caacttgctc
      181 tgcatcggcg agaaacaagt cttcgccgtc gcgcaagtct tcgatcagtt gatggaagcc
      241 atgacgcgcc atggcggctt tcgcctcacg gcctcacagg tcgacgcctt gacccaggcc
      301 gcctttcagc gggggtctga tggcgcattg cacgtcaata aagacctgat cggccaggac
      361 ccggccgtgc tcggtcgcca cgcgggagtt caggtacccc agggaacaga gctcctcttc
      421 ggggaaaccg gtgccgagca tgccttcgtc gatcacgagc agatgatgcc gttcgtgccg
      481 ttcgtacgcg tctccaacgt cgatcgtgcg atcgtcctgg ccaaggagag cgagcacggc
      541 ttcggccaca cggctgtcct ccactcacgc gatacttcag tgatggcccg gatgggcaaa
      601 atcatggact gcacaatctt cgttgtgaat ggcccttcca cggccggact tggcatgacg
      661 ggcgaaaagg tcctctcgtt ttcgatcgcc ggtccgaccg gcgaaggggt cactacgcca
      721 ctgacattct gccgccagcg acgcacgacg atcgccggca gcatgcgatt tatttgaggg
      781 gggccacatg cagctcgggc gagtggtcgg tacggcgatc gccacggtca agcatccgac
      841 cttacaggcg gagcggatgc tggtggtgca gctcgaaacg gccgcgggcc agcccgacgg
      901 cgagccgatc ctggcgttcg accggctggg ggcggggcgg ggtgatcggg tgctcgtgac
      961 caacgacggg cgagcccttc aagaattact gggggcgacc acaccgggcc gctggagcgt
     1021 gatggggctt cccgacgaca gaagctgaag atttggggga cgcgcattga gaatcgcgga
     1081 agtgattgga cgagtgacct tgtcgcggtc caatccccag ctcaaggggg ggcggctgct
     1141 gatcgctcta ccgatgccgc tggcggcttt gaccgaggcc gacccggcgc gcggtgagga
     1201 agtcgtcgtt tacgacatcc tcggcgcggg ccctggcagc ctgatagccc tctccgaagg
     1261 ccgcgaggcc gccaatccgt tcggaaaaac caagacgccg gtcgacgcct actgcgcgtg
     1321 tttgatcgac gaactttctt tttgaacatt ccatgtggga tcgagactgg attcaacggc
     1381 ataggacgat cgcgagggac acatgatcgg caacggcacg gtgatgaacg aatggaagtt
     1441 tcgcgagcag atgtgcgaaa tcggccgccg gatctacaac aagggattcg ccgcggccaa
     1501 tgacggcaat atcagcttgc ggctgggcga cgaccgggtg ctctgctcgc cgacccgcgt
     1561 gtccaagggt ttcatgaaac ccgacgatct ctgcatcgtc gacctcgatg gcaagcagat
     1621 ctccggcaaa cgcaagcggt cgagcgagat cctcttgcac ctttcgatca tgaaggcgcg
     1681 ggccgatgtg cgttcggtcg tccactgtca tccaccccac gcaaccgcct tcgccgtggc
     1741 gcgcgagccg atacccaagt gcgttttgcc ggagatcgag gtgttcctgg gcgaggtagc
     1801 catctcgcct tacgaaacac ccggtggcca gtcgtttgcc gacaccgtgc tcccctacgt
     1861 caaggacacg gaaaccattc tgctggccaa ccacggcact gtcacctatg gcaccgacct
     1921 cgaggacgcc tacttcaaga ccgaaatcat tgacgcgtat tgccggatct tgatcctggc
     1981 caagcagatt ggcagggtga attattacac cgacgagaaa gcagccgagc tgatcaagct
     2041 caagcccaag ctgggcatac ccgacccgcg cctcgagcgg ggtctcgaga attgcgacct
     2101 gtgcggcaat agtctcttcc gcgaaggtta cggcggggcc aaccccgagc cacatgtgtt
     2161 catccatccc aagatcgcgg gcagtgagcc ggtcacgtcg ccgatcgcag gggtattgac
     2221 gccgctctac agcgatctcg gcaagtctgc agcggttcaa gcttcgcctt cacgcgtggc
     2281 atccgcgagc aagccgtcca ataacggcca caccggtgcg ggctcgctcg atcccgatgc
     2341 tctcgtccag gcgatcaccg atcaagtaat ggcagcgctc aacggcgccc tgaagtaaac
     2401 aatcgctgcg cgggcagctt gtccgtggtt ctttcggttc gcgtacctgc cccgaggggg
     2461 ctcgacaaat cagcccaggg caccgccctg ggaggggaag ccatgaaagt cagcatcatc
     2521 ggcggcggcg gcctggtcgg atcgtccgcg gccttcgcgc ttcaatgcgg cgccgtcgtc
     2581 agccagatcg acctgatcga cttgaacgcc gaccaggtgc gtggacaagc ccttgacctg
     2641 ctccacggcg cgtcgctcgt cgcggatcag cggatccggg cgacaggata tgaggcaatc
     2701 cctgaaagcg acgtcatcat tatcaccgcc ggcctgcgcc gtaagccgga cgagagccgg
     2761 ctcgacttga tcaatcgcaa tgtcgagctc ttcctgggaa tcctcggcca ggccaccgcg
     2821 gccggcatca agcgcgacgc gatcgtgctg gtcgtttcca acccggtcga tgtcctgacg
     2881 tatctggccg ttcggctgct cgacctgccc tcgagccagg tgatcggcct gggaacccag
     2941 ctcgacaccg cacggtttcg cagcttgatt gccgaaaaat tgcagctccc gcccacccag
     3001 gtcacggccc tcattctggg cgagcacggc gatagcatgg tgccgatctg gtcgacggcc
     3061 caggccgccg gcctgccgct cgagcgattc gccggctgg
//
LOCUS       DATAQL010001414         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5337239, whole genome shotgun sequence.
ACCESSION   DATAQL010001414 DATAQL010000000
VERSION     DATAQL010001414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5337239"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VHS97_24430"
     CDS             complement(<1..524)
                     /locus_tag="VHS97_24430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451423.1"
                     /translation="MFKTTKNASSTHRRMRRGMLVTTSLAACVVASFGAVVPAQTISR
                     EQLIFLTAEWKGERFSDGRPKVPDSVLARMRKVSIEEAWGYMRSHGYPLQFEGDWKTI
                     HDNIPVVGRALTAQYVPARAELAKRMLDRGHQEGRVGAMNSWPIDALQPGDVYVADGF
                     GKIHEGTLIGDNLG"
     gene            complement(847..2499)
                     /locus_tag="VHS97_24435"
     CDS             complement(847..2499)
                     /locus_tag="VHS97_24435"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX3451424.1"
                     /translation="MSISPHLALAFGILAAASDGHAPENDSIRRDHLKADLIFLAGDA
                     FRGRLTATPENSLATEFVASRFSRLGLRPVTNDGSFFLRYNLITARADSTGSLEAARG
                     RSTRKFFQGADFYPHRFSASGVAHGKFAFAGFGIVAPDQGRDDYKGLDVRGRVVLVLD
                     HEPGETDPASLFDGVVTSEHADPLKKAIEAERKGAVALLIVSDLHNHPDPENFEALAA
                     AYWPASPPRIERYALQSRVEQVSIPVAQVSRSVACELLHGLNESLVALSRSAESSGGA
                     GAVAVDDVIVTLSSAVRRHVVPDRSVIAGLQGSDPKLKDQWVVISSHHDHEGADGAVI
                     FNGADDNGSGTIGLIAIAEAYASAAGKGQLPRRSILFASFNSEERGLLGSWAFVERPP
                     IPLAKIVAILNMDMVGRDEEVPEGGGPKFRGLPVQKAETNRNTFTLLGHSRSTALAES
                     IERSNATGGFGLRIKKDYDNNVSNLIRRSDHWPFLQYGVPGVWFHAGLHPDYHTANDR
                     PEKIRYDKMEQIVRLVHQASWDLAQQEDRPRLGEMHVRLHGQ"
     gene            complement(2575..3717)
                     /locus_tag="VHS97_24440"
     CDS             complement(2575..3717)
                     /locus_tag="VHS97_24440"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX3451425.1"
                     /translation="MTDSTTVVTVLGGYGIFGGRVAEALARDQICRVRIVGRSAKIGG
                     NFAHRVGADFYPSTLEDRDALCRAIDGSFLVIHAAGPFQGTDYHVAEQCINAGAHYLD
                     LSDAREFVAGIGRLDDDARRRRLMVVSGVSSTPAITSAMIAELIPEFSQIDEIHTALS
                     PGNQNPRGAATIAAVLSYLGRRIRVWRDGQWVERPGWGDSERIEFPLPVGRRRVYNCE
                     VPELELFPKIFAAKTVRFSAGLELNLLNYLLSFCGLQSRAFGLDFTRHARIFLKVSLM
                     LFPWGTTNGALAIWVRGLDHHGGPIERRIALVTDYDGPATPSSAAVVLARKILINGAP
                     RTGAFPGVGLLSLGELLAHLHPLGIWCARGDSSGWSIRLGSSHETI"
     gene            complement(3714..>4052)
                     /locus_tag="VHS97_24445"
     CDS             complement(3714..>4052)
                     /locus_tag="VHS97_24445"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEX3451426.1"
                     /translation="PVHLEVDSLEDREVWHRHFPGQTLRSVQWARGHLLMERIGLWSF
                     SSTLEVRGSRVDYVFGRSWFLGVPVPHGLAPFVESYADAGDSGWLVAVHIVAPCLGEI
                     VDYEGWVEPE"
BASE COUNT          807 a         1265 c         1222 g          758 t
ORIGIN      
        1 ttgccgagat tgtcgccgat caaggtccct tcgtggatct tgccgaaacc gtctgctacg
       61 tatacgtcgc caggctggag agcgtcgatg ggccaggaat tcatggcacc gacgcgtcct
      121 tcctgatgcc cgcggtcaag catgcgcttg gccaactcgg cgcgcgcggg cacgtattga
      181 gccgttaggg cgcggcccac aacgggtatg ttgtcgtgaa tcgttttcca atcaccctcg
      241 aactgcaaag gatagccgtg cgagcgcata tagccccatg cctcttcgat cgagaccttc
      301 cgcattcgag ccaggaccga atcgggtacc ttgggccggc cgtccgagaa acgttcgccc
      361 ttccattcgg ccgtgagaaa aatcagctgc tctcgggaaa tggtttgagc cggaacaacc
      421 gcgccgaaac tcgcgacgac gcacgctgca agcgacgtgg ttaccaacat gccgcgccgc
      481 atgcgccggt gagtcgacga tgcgttcttg gttgtcttga acatgtctag catctcctga
      541 ggacggtttt cgcgggatga cgttttcggg cgtaatttga agacgcctcg cgaaacgcca
      601 cttcgttttc cttgacccca tccctcgccc cgccggcagc gatttttcca atgaccctga
      661 ttttcacccg tcccggagca cttgtctagg gcgtggcgca gcggttgtct ggggcgtggc
      721 ggagcgggac aaggctggtc aaaccccccc gggggaaacc atgctggcct aacccaatag
      781 cgggctccga cagactcctc gcgtcggttc agtggttgcc atagtcggta taagctcctc
      841 gatcaattac tgtccatgaa gacgtacatg catctctccc agtcgcgggc ggtcctcttg
      901 ctgggccagg tcccagctcg cctggtggac tagccgcacg atctgctcca tcttgtcgta
      961 acggatcttt tcgggacgat cgttcgcggt gtgatagtcg gggtgcaagc cggcgtggaa
     1021 ccagaccccg gggacgccgt attgcagaaa cggccagtgg tcacttcggc ggatcaggtt
     1081 ggacacgttg ttatcgtaat ccttcttgat tctcagtccg aatccaccgg tggcattaga
     1141 gcgctcgatg gattcggcca gcgcggtgct tcggctatgg cccaaaagtg tgaacgtgtt
     1201 gcgattcgtc tccgccttct ggacagggag tccgcggaac ttcggcccac ccccctcggg
     1261 tacctcctca tcgcggccaa ccatatccat gttgaggatt gccacgatct tcgcaagcgg
     1321 gatcggaggc ctctcgacga acgcccatga gccgagaagt ccacgttcct cggagttgaa
     1381 cgacgcgaac aagatcgacc ggcgcggcag ctgtcccttg ccggccgcgc tcgcgtaggc
     1441 ttcggcgatc gcgatcagac cgatggtgcc ggaaccgttg tcgtcggccc cgttgaagat
     1501 caccgcgcca tcggctcctt cgtgatcgtg gtgcgaactg atgacgaccc actggtcctt
     1561 gagcttcggg tctgatcctt gaaggccggc gatcacgcta cgatccggga cgacatgtcg
     1621 ccgcacggcc gacgaaagcg tcacgatcac gtcatcaacc gcaactgccc ccgcgccgcc
     1681 gcttgattca gccgagcgag acagtgcgac caaggattcg ttcaaaccat gcagcaactc
     1741 gcaagcgacc gatcgactga cctgagctac cgggatcgag acctgttcga cgcgcgattg
     1801 gagcgcatat cgttcgatcc tgggcggact ggccggccag taagcagcgg caagagcctc
     1861 gaaattctcg ggatccgggt gattgtgaag atccgatacg atcagcagcg cgaccgcccc
     1921 ctttcgctct gcctcaatgg ctttcttcag cgggtcggcg tgctcggaag tgacaacacc
     1981 gtcgaacaga cttgcggggt cggtttcgcc gggctcgtgg tcaagcacaa ggacaactcg
     2041 cccccgcacg tcaagcccct tgtagtcatc gcgtccctgg tccggagcga cgatcccaaa
     2101 gccagcgaat gcgaacttgc catgcgccac gccgctggcg ctgaagcgat gtgggtaaaa
     2161 gtcagcgcct tgaaagaatt tacgggtaga ccggccgcgg gccgcttcga gactaccggt
     2221 cgaatcggcc ctcgccgtga tcaaattgta gcgcaggaag aacgatccgt cgttcgtaac
     2281 cggtctgagt cccaggcgag aaaaccggga ggcgacgaac tcggtggcaa ggctgttctc
     2341 gggggtcgcg gtgaggcgcc cgcgaaaggc atctcccgcg agaaagatca ggtccgcctt
     2401 cagatggtcc ctgcggatgg aatcgttctc gggcgcatgg ccgtcggacg cggccgcaag
     2461 aatgccaaac gcgagggcga ggtgcgggga gatgctcacg gcgtggtctc cgtctgcgat
     2521 tcggggtcgt gatgaagacg atggtgcttg tcggtcactt aaaaccaaga cgctctaaat
     2581 agtctcgtgc gacgatccga gccgaattga ccagcctgaa ctgtcgccac gcgcgcacca
     2641 gattcccagc ggatgaaggt gcgccaacag ttcgccgagc gacaacagac cgacgccagg
     2701 aaatgccccg gttcgcgggg ctccattgat caagatcttc cgcgccaaga cgaccgccgc
     2761 cgagctgggc gtggccgggc cgtcgtaatc ggttacgagc gcgattcgtc gctcgatcgg
     2821 cccgccgtga tggtccagtc ctcggaccca gatcgccagc gccccgttgg ttgttcccca
     2881 gggaaagagc attagcgaga ctttcaaaaa gatgcgcgca tgacgtgtga aatccaggcc
     2941 aaaggcgcgg ctctgaagtc cgcaaaacga caggaggtaa ttgagcaggt tcaactccag
     3001 tccggcggaa aagcgaaccg tctttgctgc gaagatcttc ggaaatagct ccagctcggg
     3061 cacctcgcag ttgtagacgc gtctgcgtcc aaccgggagg ggaaattcaa tgcgctcgga
     3121 atcaccccaa cccggccgtt cgacccactg gccatcgcgc catactcgga tccttcgtcc
     3181 caggtacgat aggactgccg caatcgtggc tgcgcctcgc ggattttggt ttcccggcga
     3241 cagagccgtg tgaatttcgt cgatctggga gaactcgggg atcagctcgg cgatcatcgc
     3301 tgaggtaatc gcgggagtcg aactcacccc ggagacgacc attaaccgcc gccggcgggc
     3361 gtcgtcgtcg agccgaccta tacccgcgac aaactcccgt gcgtcggaca ggtccaggta
     3421 atgcgcgccg gcattgatac actgctcggc cacgtgataa tccgtcccct gaaacgggcc
     3481 agcggcgtga atgaccagaa acgacccgtc gatcgctcgg cacaaggcgt cgcgatcttc
     3541 gagcgtcgag gggtaaaagt cggcaccgac gcggtgcgcg aagttaccgc cgatcttggc
     3601 gctgcggcca acgatgcgga cgcggcagat ctggtcacgc gccagtgcct cggcaacgcg
     3661 gcctccaaag atgccgtagc cgcccagcac agtcacgacc gtcgtggagt ctgtcattca
     3721 ggctcgaccc acccctcgta atcgacaatc tcacccaggc acggcgcaac gatgtgaaca
     3781 gccaccagcc agccagagtc gccggcgtct gcatagctct cgacgaatgg tgcgagcccg
     3841 tgcggtaccg gaacgcccag aaaccacgac cgtccaaaca cgtaatcgac ccgcgacccg
     3901 cgcacttcga gcgtgctcga aaacgaccag agcccgatgc gctccatgag caagtgtcct
     3961 cgtgcccatt gcaccgacct taatgtctgc ccgggaaagt ggcggtgcca gacctcgcgg
     4021 tcttcgagcg agtcgacttc gaggtgtact gg
//
LOCUS       DATAQL010001415         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_146201, whole genome shotgun sequence.
ACCESSION   DATAQL010001415 DATAQL010000000
VERSION     DATAQL010001415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146201"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VHS97_24450"
     CDS             complement(<1..1427)
                     /locus_tag="VHS97_24450"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451427.1"
                     /translation="MNTPCISMKSKRASFALVTLILGGIVGAISRPAHGQDPPPTPSP
                     VADGADPQGAPAGDQTNPQGGQVLTRGPIHEAFAAPVVHDPASGPVISKQPPDPIEEM
                     PPDQKPAGQNVQWIPGYWSWDVSRNDYLWVSGIWRDPPPSSQWVPGYWHQVEGGNQWV
                     SGTWVPVSTNQGQGSSQGQGSGQGQPSYLPKAPPPSLESGPTTPQPNPNVAWTPGFWS
                     WQGMNYVWRPGFWAAVQPNWIWMPAHYVWTPSGYLFVAGYWDLPVANRGLMFAPVYYP
                     QPVYAQPGFVFTPSISIVGSAVTANLFVSAGTNQYLFGDFYAQNFVSVGITPWFSFSF
                     GTGRPLFYDPLFSYYAVINVRQNPRWITQVREEYVLRRDNVAMRPPHTYIEQTRLIER
                     NVTIDRNMAMPLNRMAAERGMRLERVSEAERRQVRQQVAQVHQLREQRAQQEREGARA
                     RLAGGAAASHPRPMNLPHSPIAAHPA"
     gene            complement(1654..2454)
                     /locus_tag="VHS97_24455"
     CDS             complement(1654..2454)
                     /locus_tag="VHS97_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3451428.1"
                     /translation="MYEAHFGLVERPFGETVSPAAYVALPSRDTVLRRLRYALEHGQG
                     PAVLYGAPGTGKTLLARRLANELGGPAIHITFPALSAAELVAHLAEELGGTPVQPTSL
                     GVALREVRGQLAALATRRERLLVVVDDAHLIEAVSTFEALRLLLNFATQGPPDMSLLF
                     VGGAEVLLELPPGLSDRLAARCLLPPLTETESSTYVLGRLENAGARLPLFSPDALIAL
                     HRAANGLPSHLNHLADLALLIAYAQEMSTADASLVAIAAREYNRNVAA"
     gene            complement(2472..3251)
                     /locus_tag="VHS97_24460"
     CDS             complement(2472..3251)
                     /locus_tag="VHS97_24460"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX3451429.1"
                     /translation="MREVDEALARAFAQREQNDPRPKVPPAPHWPVRSQKTVVPRAHP
                     LDAEAQAIELQWPAVVLTLEREWGERFEKMAHLMRAVQERQGFKAFLFTSCHRAEGRS
                     TLVLTLARALAREPIKTLVLDADLTGPMIARLLGLRPQVGLDDVVESGLALSDALIDA
                     RDDHLGILPMRAAVSRPRDFMSNPAWTCTMARLRREFDLILVDGGPLFAGLSAAVLHR
                     SVDAAVLVHNRGMTGERSVIRAREVLEAGGVPLLGLAETFV"
     gene            complement(3238..3852)
                     /locus_tag="VHS97_24465"
     CDS             complement(3238..3852)
                     /locus_tag="VHS97_24465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451430.1"
                     /translation="MADEIQKRAFIYQQLETRTESFELNREGGEGAAMSQSDTLSKMP
                     AIRSDRDLSLALEVLIEVPDLKGSRMGRGRTQPPSQEPISRPRYVRTKRRLRREVRVA
                     GCALLALMPLVSLCTIGSSSQPSRVVACSIADTIAGRNNLAATAPRDRALEPATNASR
                     STGAVVLSVEPPALVPGNDTEVPVVFPGYVLPDDSLEDAAHEGS"
     gene            complement(4404..>4824)
                     /locus_tag="VHS97_24470"
     CDS             complement(4404..>4824)
                     /locus_tag="VHS97_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451431.1"
                     /translation="SVELHANSGSMLNGQDDKDRIVLYEYNLVYGLDGKVDETNPMAA
                     DWIKVGGEAMFAPLNVLELVESRWGGHNQQVTEANVRSIDMANGGLSRGRLASSRPPD
                     FRPVAQYEAGRTQMIADSDNMIRTPRFGGFLRGFFGR"
BASE COUNT          915 a         1543 c         1505 g          861 t
ORIGIN      
        1 gctgggtgcg cggcgatcgg cgagtgtggt aagttcatcg gccgagggtg agacgcggcc
       61 gcgccccctg ccaaccgcgc ccgtgcccct tcccgctctt gttgcgcccg ctgctcacgc
      121 agctgatgca cctgcgcgac ctgttggcgc acctgccggc gctcggcttc gctcacccgc
      181 tcgagccgca tgcccctttc ggccgccatc ctgttgagcg gcatcgccat gttacgatcg
      241 atcgtgacat tgcgctcgat cagacgcgtc tgctcgatgt acgtgtgcgg cggccgcatc
      301 gcgacattgt cacgtcgcaa tacgtactct tcacgcacct gggtgatcca gcgcgggttt
      361 tgccgaacat tgatcacggc gtagtacgaa aacaatgggt cgtaaaagag cggccgaccg
      421 gtcccgaagc tgaaggaaaa ccagggagtg atcccgacgc tcacgaagtt ttgagcgtaa
      481 aagtcaccga aaagatactg gttcgtgccc gccgaaacga agaggttggc ggtaacggcc
      541 gatccgacga tgctgatcga tggcgtgaag acaaaacccg gctgcgcgta aacgggttgc
      601 ggataataga ccggggcaaa catcagccct cggttcgcga ccggcaagtc ccagtagccg
      661 gcgacgaaga ggtagccgct gggcgtccag acgtagtgag caggcatcca gatccaattg
      721 ggctggacgg ccgcccagaa tccggggcgc cagacgtaat tcattccttg ccacgaccag
      781 aagcccggag tccaggcgac gttcggattc ggctggggag tggtcggacc cgattcgagg
      841 ctcggcggcg gcgctttggg aagatatgac ggctggcctt ggcccgaacc ctggccctgg
      901 ctcgaaccct ggccctgatt cgtgctgacg ggcacccacg ttcctgagac ccactggtta
      961 cctccttcaa cctggtgcca gtagccgggc acccactggc tactgggcgg agggtcgcgc
     1021 cagatgccgc tgacccagag gtagtcgtta cgcgagacgt cccagctcca gtatcccggg
     1081 atccattgga cgttctgacc tgcgggcttc tggtccggcg gcatttcctc gatcggatcg
     1141 gggggctgct tcgagattac cggtcccgag gccggatcgt gaaccaccgg cgcggcgaac
     1201 gcctcgtgga tcgggccgcg tgtcaagacc tgtcctccct ggggattggt ctgatctccc
     1261 gccggcgccc cttgtggatc agctccatcg gcgaccggac tcggagtagg gggagggtcc
     1321 tgtccgtgtg ccggccggct tattgccccg acgattcccc cgagaatgag cgtcaccaaa
     1381 gcaaagctgg cacgcttcga tttcatagaa atgcaaggtg tattcatcag agccctcctt
     1441 gactcggcaa gaccgcgaat ccgtcgaatt caatcggcgg cgcggatcgc ccaagttcgt
     1501 cgccagttcc ttcgcggtcg tgcatccgca ggtccgggat tctaccttcc gggcatgaat
     1561 tcgtctgccg gattcgccag agtttcagca ccccgttgcc gcgaatcgaa agatagagaa
     1621 acctggacga ttgcatgtcc ctgagaaaga tcgtcaggcg gcgacgtttc ggttgtattc
     1681 gcgggcggca atagcgacga gcgaggcgtc ggcggtggac atctcctggg cgtacgcgat
     1741 cagtaaagca agatcggcca gatggttaag gtggctaggc agcccattgg cagcgcgatg
     1801 gagtgcgatc aaagcatccg gcgaaaatag aggcaaccgg gcgccggcat tttcgagccg
     1861 tccgaggacg taggttgacg actcggtttc cgtgaggggt gggagcaggc agcgggccgc
     1921 gagccgatct gacaggccgg gaggaagctc gagtaagacc tccgcgccgc cgacgaaaag
     1981 aagcgacatg tcaggaggtc cttgggttgc gaagttgagc aacagccgca gcgcttcaaa
     2041 agtggagaca gcctcgatca ggtgggcatc gtcgacgacc accagcaggc gctcacgccg
     2101 cgttgccagc gcggcgagct ggccgcgcac ttcccgcagc gcgacaccaa gcgatgtggg
     2161 ttgaaccggc gtaccaccaa gctcttcggc cagatgcgcc actagttcag ccgcggaaag
     2221 ggccgggaac gtgatgtgta ttgccggacc ccctagctcg ttcgccagcc ggcgcgccaa
     2281 gagcgtcttg cccgtgcccg gtgcgccgta cagcactgcg ggcccctggc cgtgttcgag
     2341 tgcataacga agcctgcgca gcacggtgtc gcgactcgga agtgcgacat aggcagccgg
     2401 gctcaccgtc tcgccgaacg gacgctcaac caggccgaag tgcgcttcgt acacggggcc
     2461 tccaccgtga gtcagacgaa ggtctcggcc aaacccagga gcggcacgcc cccagcctcg
     2521 agcacctcgc gggcgcgaat gaccgagcgc tcaccggtca tgcctcgatt atgcaccagg
     2581 accgcggcat cgacggaccg atggagaacc gccgcgctca agcccgcaaa gagtgggccc
     2641 ccgtccacca gaatcaggtc gaattcccga cgcaggcggg ccatcgtgca ggtccaggcc
     2701 ggattggaca taaagtctcg agggcgcgag accgccgccc gcatgggcaa gatccccaga
     2761 tggtcgtcac gcgcgtcgat caacgcgtct gatagggcga gcccactctc gacgacgtca
     2821 tccaacccca cttggggtcg cagacccaga agccgcgcga tcatcggccc ggtcagatcg
     2881 gcgtcgagca ccaacgtctt tatcggctct cgcgccaggg cgcgcgccag ggtcaagacg
     2941 agcgtcgagc gtccctcggc acgatggcaa ctggtgaaca gaaaggcttt gaacccctgc
     3001 cgctcttgga ccgcgcgcat cagatgcgcc attttttcaa accgttcgcc ccattctcgt
     3061 tcgagcgtga gcactaccgc cggccattgg agctcgatcg cctgagcctc cgcgtccagt
     3121 ggatgcgccc gtgggaccac ggtcttctgt gagcggactg gccagtgagg tgccggcgga
     3181 accttggggc gagggtcgtt ctgctcacgc tgggcaaagg ctcgagccag cgcctcgtca
     3241 acttccctca tgcgctgcgt cctccaggct atcatcgggc aacacgtagc cggggaacac
     3301 cacggggact tccgtgtcgt tgccaggaac cagcgctggc ggctcgaccg acagcacgac
     3361 cgccccggtc gacctcgagg cattggtcgc gggctccaac gctcggtcgc gtggggccgt
     3421 tgcggccagg ttgttgcgtc cggcgatcgt gtcggcaatc gaacacgcga cgacccggct
     3481 gggctggctc gacgacccga tcgtgcacag agagacgagt ggcatgagag ccaagagggc
     3541 gcaacctgcc acgcgaacct cgcggcggag ccggcgcttg gtgcgcacgt atctcggccg
     3601 cgaaatgggc tcttgactgg gcggttgcgt tcttccacgc cccatccggc tgcccttcag
     3661 atcaggcact tcgatcaaca cctcgagcgc gagcgaaagg tctcgatcgg atcgaatcgc
     3721 cggcatcttt gagagagtat cggattgact catcgcagca ccctcgcccc cctcgcggtt
     3781 cagctcgaag ctctcggtcc tggtttctaa ctgttggtag ataaacgccc gcttctgaat
     3841 ttcatcggcc attgcggctc taccgattcg gtgtgtttca tcgtctggaa agctcgtcaa
     3901 acttgaacac ccttgacgaa aatgacggac ccgaaagcct gcccgatcga tttggttgaa
     3961 tggccgttat gttcaggaca accggagcgg cataatcgct acattccctc acccgacagc
     4021 acggaaccgc ccgattaaaa gtgggaggcc cggcccgggt ggcctggcgt aaaccagact
     4081 ccgggtcggg cctcgtgaaa gcacagcggg gcaaaagatc accccgtgcg ctcgaacata
     4141 agaatcgcga gctcctcggg cgcttcgtcg tggcacgggc ttccagccca tgtctaagaa
     4201 acccacgggc aagatgcccg tgccacttgc tcgggcgctt cgtcgtggca tgggcttcca
     4261 gcccatgtct aagaaaccca cgggcaagat gcccgtgcca cttgctcggg cgcttcgtcg
     4321 tggcacgggc ttccagccca tgtccaggaa acccacgcgc aagatgcccg tgccagctgc
     4381 tcaacttatt tttctccggt cccttagcgc ccgaagaacc cacgcaggaa tccgccaaac
     4441 cgcggagtgc gaatcatgtt gtcgctatcg gcaatcatct gggtccggcc ggcctcgtac
     4501 tgggcgaccg gccggaagtc cggcggcctc gacgaggcca ggcgtcctcg actgaggccg
     4561 ccatttgcca tgtcgatcga ccggacgttt gcttccgtca cttgctggtt gtggccgccc
     4621 cagcgcgatt ccaccaactc gagaacgttg agtggagcaa acatcgcctc gcccccaact
     4681 ttaatccagt ccgcggccat ggggttggtc tcgtcgacct tgccgtccag gccgtaaacc
     4741 aggttgtact cgtagaggac gatgcgatcc ttgtcatcct gaccgttcaa catcgagccc
     4801 gaattcgcat gcagctcgac ggag
//
LOCUS       DATAQL010001416         5211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5184427, whole genome shotgun sequence.
ACCESSION   DATAQL010001416 DATAQL010000000
VERSION     DATAQL010001416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5184427"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VHS97_24475"
     CDS             complement(<1..533)
                     /locus_tag="VHS97_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX3451432.1"
                     /translation="MPRIKQMLSEGKSVRVFGVGQLVSPKFIEILGEHGEFDALWLDF
                     EHGGLTMKEIELATMAARSYGMDHFVRLPATDYATVMRPLEAGAGGVMISMVQSPADV
                     EKAVRWAKFWPRGERGLIGGNRDGRIGLTPIAEYTARANAETFFGVQIETAGALASLN
                     EIAAVPDVDLLFVGPSDL"
     gene            694..1380
                     /locus_tag="VHS97_24480"
     CDS             694..1380
                     /locus_tag="VHS97_24480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451433.1"
                     /translation="MPPASNKQLDIDARWLYWRMFLLFFFVFEMIVWQRLQRFPIRAP
                     GHRMIVFLFARGATPRSLMRAIFAGGVFTLLTTLIVRLIVRPLLNFWLRPTADSSGGL
                     FHLTASETIVANVPARRRTGWVWKPGSLALTNRRLWFFPAGFDDEPWFLPLNDVADVV
                     PERPLLGELAPLRNWPEYLRVSARSGPDAIFAVADPSAVLAWLNFPAGRDAAALLAAG
                     AGHPAGVFDE"
     gene            1373..2353
                     /locus_tag="VHS97_24485"
     CDS             1373..2353
                     /locus_tag="VHS97_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX3451434.1"
                     /translation="MSDRFTVLIADFLDETSIESVILDDIARIVMANAKDEAELANYL
                     PEADAILLFHAISILGEQSFARAPRCRCVVRAGVGYNNIDLAAATRHCVIVCNVPDYG
                     TEEVADHTIMLLLAIVRRLVPSHMAIRAGTWDYRTAVGAPRLRGKTFGVVGCGRIGTA
                     TALRARSLGLDVVFYDPFLRQGMDKALGIRRVFQLEELLEQSHFVSLHCYLDSTTRHI
                     INERTLALMRPGAVLINTARGPLVDEKALLDALDSGHIASAALDVVESEPLENDRIRS
                     HPNILLTPHTAFYSVEGYTELRTKTAQEVRRVLLGEPPRNPVNAPSARTL"
     gene            2386..3840
                     /locus_tag="VHS97_24490"
     CDS             2386..3840
                     /locus_tag="VHS97_24490"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX3451435.1"
                     /translation="MSFDDSRYGNRLSQGTDSQPWLSWIVVVTLTAFLTAVSTSQALH
                     QYQKLHSGWSWDLAYYNQWFWSLTQGDGTITVRPVSAYAQEGPSVWKMNYLAPIRLVL
                     APVYRLNPGPIVLLVIQNVMFWWVIPAAFTLVRSETRSDAVALSAAALVPLTPLLWPL
                     VWNDFRELQLAGPFVLWAVQGVRSRSAGWAAIGIAGMLACRQEYAIMVATFAFLPPRE
                     PESLSRTLLWRRTVFLVGVVWLVVGFLGYLRFTAGPKTSELYIDQFLGPKASFQEAGA
                     TSLETLVVGMGAWALLACLVPRIAILALPWIWGPCGGNWAMRLLSTSEWHAVRYVMPM
                     AAILLAAGLIGYARLATALLARRGGRAGLAVVWVCAAVTFGMGLRDITDRLAHAPVLI
                     DREDAEQIWYWIKQVGDDDAVMADYEVSAPLSSRRRIYSYIMDVNLPKNFPELDSDFR
                     WLFIRKGYPLLNRLLEQGFEVVCQGKYLTIARRS"
     gene            complement(4914..>5211)
                     /locus_tag="VHS97_24495"
     CDS             complement(4914..>5211)
                     /locus_tag="VHS97_24495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451436.1"
                     /translation="LRSPDHDRQIGPPAALEIELMRTVEHVNHLPRYRGLTRIPLSIS
                     RGASCRGVGMNPGDIMIVFSLRADVTAVRVSVSLSSRTRHERRNRVRSKRHGDQ"
BASE COUNT          933 a         1593 c         1572 g         1113 t
ORIGIN      
        1 aggtcggagg gtccgacgaa cagcagatcg acgtcgggca ccgccgcaat ctcgttcaaa
       61 gacgcaaggg cccccgccgt ctcgatctgg acgccgaaaa aggtctcggc gttggcccgg
      121 gcggtgtatt cagcgatcgg agtcaagccg atccggccat cgcgattgcc tccgatcaaa
      181 ccacgctcgc ctctgggcca gaatttggcc caccgcacgg ctttttcgac atccgcgggc
      241 gactggacca tactgatcat gactcccccg gcccccgcct cgaggggccg catcaccgtc
      301 gcgtagtcgg ttgccggcag ccgtacgaaa tgatccatgc cgtagctccg ggccgccatg
      361 gttgccagct cgatctcttt catggtcagc cccccatgct cgaagtcgag ccagagagcg
      421 tcgaactcac catgttcacc caggatctcg atgaatttgg gactcacgag ctgtcccacc
      481 ccaaagaccc ggaccgactt gccctcgctg agcatctgct tgattcgtgg catcccaata
      541 cttccctcac tagggcgact ataggacaat ggatcgatcg tgcgcgcttg gctttggcac
      601 gcacttaaac acctggcttg aaactacccg gccggccgtt ccggaccaag gggtttctcg
      661 ccggcgccag acgaaggata agggctaatt gcgatgccgc cggcgagcaa caagcaactg
      721 gatatcgacg cacgctggct gtactggcgg atgttccttc tctttttttt cgttttcgag
      781 atgatcgtct ggcagcggct ccagcgattt ccgatccgcg cgcccgggca ccgaatgatc
      841 gtgtttctgt ttgcccgcgg ggccacgccg cgctccttga tgcgggcgat cttcgcggga
      901 ggcgtgttta cactgctgac gaccctgatt gtccggctga tcgtccgccc gctcttgaac
      961 ttctggctgc ggccaacagc tgactcatca ggaggactct ttcacttgac cgccagcgag
     1021 acgatcgtcg cgaatgtgcc agcgcggcgc cgaacgggat gggtgtggaa acccggatcg
     1081 ctggccctca ccaatcgtcg actctggttt tttcccgcgg gatttgacga cgagccctgg
     1141 ttcctgccac tcaacgacgt tgcggatgtc gtgccggagc ggcctttgtt gggcgaactg
     1201 gcgcccttgc gcaactggcc ggaatactta cgcgtgagcg ctcgttccgg cccggatgcc
     1261 atcttcgcgg tcgccgaccc aagcgccgtg ctcgcctggc tcaacttccc ggcggggcgc
     1321 gacgccgcgg cactactcgc ggccggcgcc ggccatccgg caggagtgtt cgatgagtga
     1381 tcgattcacg gtgctgatcg ccgatttcct cgatgagact tcgattgaat ccgtaatcct
     1441 cgacgacatc gcacggatcg tcatggcgaa tgccaaagat gaggcggagc ttgcgaatta
     1501 cctgcccgag gctgatgcga tcttgctttt tcacgctata tcgatcttag gcgagcagag
     1561 tttcgcgcgc gcgccgcgct gccggtgcgt ggtccgtgcg ggcgtcggct acaacaatat
     1621 cgacctcgcg gcggccacac ggcactgcgt gatcgtctgc aacgtgcccg attatggaac
     1681 cgaggaggtg gccgaccaca cgattatgct tttattagcg atcgtgcgca ggctggtccc
     1741 ctcgcatatg gccattcgcg cagggacctg ggactaccga actgcggtgg gcgctccccg
     1801 gctccgaggc aagacgttcg gcgtggtcgg ttgcggtcgg atcggcacgg cgacggctct
     1861 ccgcgctcgg tcgctggggc ttgacgtcgt cttttacgac ccatttctgc gacaaggcat
     1921 ggacaaggct ttggggatcc gccgcgtgtt tcagctcgag gaattgctcg agcaaagcca
     1981 ttttgtcagc ctccattgct atctagattc gacgaccagg cacataatca acgagcgcac
     2041 gctggccttg atgcgacctg gggcagtgct catcaacacc gcccgcggcc cgctggtcga
     2101 tgagaaagca cttctggatg cgctcgactc gggccatatc gcatccgccg ccctcgacgt
     2161 tgtcgaaagc gagccgctgg aaaacgaccg catccgcagc caccctaaca ttctgctcac
     2221 gccccacacg gcgttttaca gcgttgaggg ctacaccgag ctgcgcacca aaaccgctca
     2281 ggaagtaagg cgggtcttgc tgggcgaacc gccgcgaaac ccggtgaatg cgccgtccgc
     2341 acgaacgctt tgatgtgctc agcaaacaaa cgtggcgaac ttgggatgag ttttgatgat
     2401 tcacgatacg gcaatcggtt aagccaaggc acagattccc agccctggct gagctggatc
     2461 gtggtcgtaa cgctgaccgc ctttttgacc gccgtctcga catcgcaggc gctgcaccaa
     2521 taccaaaagc tgcacagcgg ctggtcgtgg gatctggctt attacaacca gtggttctgg
     2581 tcgctcacgc agggggatgg gaccatcacc gtccgaccgg tctcggcgta cgctcaggag
     2641 ggtccgtccg tctggaagat gaattatctg gctccgatcc ggctggtgct cgcaccggtc
     2701 taccggttga atcctggtcc gatcgtgcta ttggtcattc agaatgtgat gttctggtgg
     2761 gtaatcccgg cggcattcac gctggtgcgc tccgagactc gatccgacgc cgtggcgctt
     2821 tcagcggccg cgcttgttcc cctgacgccg ctcctctggc cgctcgtctg gaacgatttt
     2881 cgtgagctgc aactcgccgg ccctttcgtt ttgtgggccg tacagggggt gcgaagccgg
     2941 tcggcgggtt gggccgcgat tggaatcgcc gggatgctcg cgtgccggca agaatacgcc
     3001 atcatggtcg cgaccttcgc gtttttgccg ccgcgcgaac ccgagtcgct cagtagaacg
     3061 cttctctgga ggcggacagt cttcttggtc ggtgtcgtct ggctggttgt cgggtttctg
     3121 ggctatctga gattcacggc cggccccaaa acctcggagt tgtacatcga tcagtttttg
     3181 ggtcccaagg cgtcgttcca ggaagccggg gctacctcgc tcgagaccct cgtggtggga
     3241 atgggggcat gggcgctcct ggcatgcctg gtgccgcgga ttgcgattct cgccttgcct
     3301 tggatctggg gcccttgtgg aggaaattgg gcgatgcggc tgctgtcgac ttcggaatgg
     3361 catgccgtcc gctatgtgat gccgatggcc gccatcttgc tggctgccgg cttgatcggc
     3421 tacgcgcgcc tggcgaccgc gctcctggcg cgacgcggcg gccgagcagg ccttgcggtc
     3481 gtctgggtct gcgcggccgt gacctttggc atggggctcc gagacatcac cgatcggctg
     3541 gcccatgccc ccgtcctgat cgatcgcgag gatgccgaac agatctggta ttggatcaaa
     3601 caggtcggcg acgacgacgc cgtcatggct gactacgagg tctcggctcc cttgtcgtcg
     3661 cgacgacgga tttatagcta catcatggat gtcaacctgc ccaagaattt tcccgagctc
     3721 gattctgact ttcggtggct gtttatccgc aagggatatc ctttactcaa ccggttgctg
     3781 gaacaggggt ttgaagtcgt ttgccagggc aaatacctga cgattgcccg gcgctcctag
     3841 ggctctctct gtccgagatt ccgaattttt ccgattttgc gcgaacacaa accctcgata
     3901 agatgtctat agaccgtacc gccggacagg gagcgctctc agattgggcg atccttgaaa
     3961 taggcggtgc tgaatcgaac ttgtgatttg ttcggccggg tgtgttgccc ggccggatcg
     4021 aggtatcggg agctccgacg gaccccgtga ctcggtgact gatcgaggga tgcccaagtc
     4081 gtcccaattc ccgtgatgcg atagcccgat tttctgcaaa ggctattgct tcacctcgtg
     4141 accccgccct ttctgcggcg gatgtgaggg tctcgccatg ttgaaccgca cgaacctgac
     4201 gctgtcggcc ttggcacttt ccaaccggct gaatctcggg acgcggtcgc tcgccgctaa
     4261 cgcgagcgtc aacttcggcg gtcagaccct acgcacccgc cagtctcatc atgacgtcat
     4321 gcgcgaaagg atggactggt atgtcgtatt atcgatcccc cgaccgacgt acgcgattcc
     4381 agccattcca ggctcatttt tccacgtcct gcaacgtcaa ttgagttttg tgacatcaga
     4441 gcaaagtccg gtcgctgtcc atgtttgcgt cgtatccgag ccgaggccag cagacctcgt
     4501 cgatcgatgt gaacgagacg tgccagttcc tgatcccagt cttcaaccga gcgattctct
     4561 cgtgggaatc gcgataccca gcgcgagtga ttgcaagtcg cgctcggcgg ttaaatcggc
     4621 tgggaaacgc ggattgaagc gcaccgcaaa gcgttccgct ccaagagcgg ccgccacgat
     4681 gtcacgaact catggaaagt gagcaccgat cgctcgattc cgtgaccttc ttccaaacgt
     4741 cccttggcac ttgactcata aggtttccag ccgggaaccg caacgagctg caaagtgcac
     4801 gcggtcgtaa ggttgaagac aaagacggac tcgccaaatc ggtttcgcac ggctggcccc
     4861 gatcaacctg gttttacttc ggaatcggac ttggccaccg cgcacgcgcc gtctcattgg
     4921 tcgccgtgcc gttttgaccg cactcggttg cgacgttcgt gccgtgtcct ggaagacaag
     4981 gagacgctca cacgcacggc ggtcacatcg gctctgagcg agaagacgat catgatgtca
     5041 cccggattca tgccgacgcc ccggcacgaa gcgccgcgag agatcgaaag cggaattcgg
     5101 gtaagccccc ggtatcgggg aaggtggttc acgtgttcaa cggtacgcat gagctcgatc
     5161 tcgagagcag ccggtgggcc gatctggcga tcgtggtccg gcgagcgcag g
//
LOCUS       DATAQL010001417         8322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3963371, whole genome shotgun sequence.
ACCESSION   DATAQL010001417 DATAQL010000000
VERSION     DATAQL010001417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3963371"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1518
                     /locus_tag="VHS97_24500"
     CDS             247..1518
                     /locus_tag="VHS97_24500"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX3451437.1"
                     /translation="MARDKVVLAYSGGLDTSVAVKWIDETYGMDVVAYTCDLGQGQDI
                     EAIRQKALKTGAIDAVAEDARNLFVDYFVWPSLAAGALYEGKYPLATALGRPLIAQLM
                     VRVARDHGAVAVAHGCTGKGNDQVRFDVTFQTLAPDLKIIAPVREWKWTRTQELEFAA
                     KHGIEVEATKKSIFSTDQNLWGRSIEAGILEDPWVEPPPETFQWTTDPRLAPDLPAEI
                     EIRFDRGKPVALDGRELDGVELIGELNQLAGKHGVGRIDHIENRLVGIKSREIYEAPA
                     AILLHEAHREVEFLTLSKEALRFKTQVSQAYADLIYNGLWFSQLHQDLMAFTVSNQQY
                     VSGTARIKLYKGHAHTVGRKSDHSLYRHELATYEEGDQYDSSAALGFIKIHGLGQTTQ
                     AKHQLLRGPGGRPLELPSIIPPSRAESPDKS"
     gene            1787..3127
                     /locus_tag="VHS97_24505"
     CDS             1787..3127
                     /locus_tag="VHS97_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEX3451438.1"
                     /translation="MPEMMTTDVRAIMDRSPFDVAAVADLRELLNRDPSRYRTLREAV
                     ANIKEREKKEKESKPETHLRLGVGEVLLGRYNSGLEHLKKAGDLGMACFFRGLALENQ
                     QRYDQAAAAFAQAAKQGYDAKTADLHRAGALRRAGNIEEAKKILASLQKHGESSPEYH
                     FQQGCVLAASGELVEASAQFEKTLSIEKDHNGALFELAYINDLYGNDDLALEYYRRCT
                     DRPPVPLAAWINLGILYEDEMRFRDAEQCYRHVLAHEPNHPRARLFVKDCQASKGMYY
                     DEEAERGYTVLKQLHEIPVTDFELSVRSRNCLRKMNIRTLGDLTRTTEVALLSSKNFG
                     ETSLAEIKDMMQAKGLRLGMSLEGNERAGHDHRVEQPQEVPPELQAIMNKPIGDLNLS
                     VRARKCMSKLNIQTVGDLLKHSGDELLECKNFGVTSLTEVREKLTSLSLKLKND"
     gene            3134..4279
                     /gene="tgt"
                     /locus_tag="VHS97_24510"
     CDS             3134..4279
                     /gene="tgt"
                     /locus_tag="VHS97_24510"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX3451439.1"
                     /translation="MRIATGASTASSTPIRFTSQAVDSGSGARLGLLETRSGSLETPA
                     FMPVGTQATVKGLTPEQLRTSGTRMLLANTYHLALRPGAEVVRSLGGLHTFMGWDGPI
                     LTDSGGFQVFSLADRTRVTDHGVTFRSHLDGRLLELTPESATAIQEALGADVAMCLDH
                     CPSLPASTAQIADAVGRTVDWARRCKAAHERRDQALFGIVQGGPHADLRAQCASELLA
                     LDFDGYAVGGVSVGEDRQTVRQALAVTTHLLPANRPRYLMGVGRPQDILDAVATRIDL
                     FDCVMPTRNGRNATCFTQTGYVKLRNAVHRLDPRPLEEGCDCLACRRFSRSYLRHLFL
                     AKEMLGPILASIHNLTFLHRLTSRIRAAIAAGRFVQFRLEVLEALGP"
     gene            4411..4764
                     /gene="yajC"
                     /locus_tag="VHS97_24515"
     CDS             4411..4764
                     /gene="yajC"
                     /locus_tag="VHS97_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX3451440.1"
                     /translation="MIGLLAEIPLLFAQDAASGSIWSLLLPLAPIPFLYYFLVFMPQQ
                     QQEKKRRAMIDGLKKNDKVLTNGGIYGTVMSVDSTNDKVVLRVDDEKGVRMAFTKSSV
                     ARVLELSAEKSTEST"
     gene            4907..7972
                     /gene="secD"
                     /locus_tag="VHS97_24520"
     CDS             4907..7972
                     /gene="secD"
                     /locus_tag="VHS97_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX3451441.1"
                     /translation="MKRFGWKIGLIVGSTLLGLAFMWPPSQKLKLGIDLSGGTILVYE
                     AENLPPGFNLDELIAALKKRADPEGVKEIPIRKIGGNRIEIILPQASNEEVEEVKKML
                     TDVGSLEFRILANGKHDKEVVGRALGAILSKPPSRYMWARLGEISNGTNPTFTSDSIT
                     DPDQSWKKDLYAGTDVYVTGKDSSGNSRTESVKVRRNTTNTLILDKPHGLKTVTSYRI
                     EYNPSHLKGGDPNNLGPNDLIVREEKVAPGRVETYILANLDRPGQEVTGKYLRQAKPD
                     VDDRLQPAVRFEFDSRGASRFGKLTREHKPEEGGAFRYQLAILLDKLVMSAPAINSEI
                     RDSGIIEGGPQGFKAKEVEHLIQVLRAGSLPASLNPNPLQEEKVGPTLGEDTIAKGVF
                     AIWVSLLVVPIFMIIYYRFAGVVAVLALLVNMILLIGSMSFIQATFSLPGLAGLALTI
                     GMAVDANVLVFERMREEKERGASLAQQIRNGFNRAWVTIFDSHVTLFLAALVLYMVGT
                     EEVKGFALTMIIGMAWNLFTAVFMSRVIFDYFYSRGWLKQVNMLKLMDKTNFDFIGPR
                     YYCMAGSVILIVLGLIATGVRGQSMFNIDFTGGTLVTIRLNESDPSVKPLTESQRAQL
                     VREKASVLPDVTVESLRVGNDKNLARFNIRTTEQDQSKVKRVVLEAFGSSLARVEMNY
                     GEAKPIAAATPPAAEAGKAAPPVVNRFAGGRQYELTFNTTAFNSEQSPAQVVSAVFAR
                     VLDNAKIANPISRFELIPGPDRPAAATAGGASTAPAAGTRLILRTDLEPEVAKVELEQ
                     LKKALADDPDLLFERSMNFGGTVAGETRQRALVATFASWLIIIAYLWWRFHSFTYGFA
                     AVLAVVHDVLIALGAIAVSYWLAKVPGLSTLLQIDQFKIDLPIVAAFLTLIGFSVNDT
                     IVIFDRIREIKGKTPHLTNKMVNDAINQTLSRTILTSFTAWLVVVILYFFGCEGLHGF
                     AFALVVGFLSGTYSTVYIATPILIDFVATKPGPPTKVGKQLAAARSLLD"
BASE COUNT         1709 a         2510 c         2503 g         1600 t
ORIGIN      
        1 gcgaggtgcg gatcaacgtc ggcacggata tcgcgcgcgg taaggagggc ctcgaatgcc
       61 tgaaacgcgc attcgccctc tagccagaga ttgacctgaa gtagacaatt agcctcggag
      121 cgaggctaaa ttgcaatgga gacctccggt cggccgacgg gcggggtcag gagatccgcg
      181 cccaacaatt agcctcgaat cgagactgga tcgaatcgca ccactgacga gacgggagga
      241 gtgagggtgg caagggacaa ggtcgtgctg gcgtatagtg gcgggctcga cacatcggtg
      301 gccgtcaagt ggatcgacga gacgtatggc atggatgtgg tcgcctacac gtgcgacctg
      361 ggacaaggac aagacatcga ggcgatccgc caaaaggcgc ttaagaccgg cgcgatcgac
      421 gcggtcgcag aggacgcacg taatctgttc gtcgattatt tcgtctggcc gtcgctggcg
      481 gctggtgcgc tttatgaggg gaaatacccg ttggcgacgg ccttgggacg tcctttgatc
      541 gcgcagctca tggtgcgtgt cgcgcgggat cacggcgcgg tcgcggtggc gcatggctgc
      601 accggcaagg ggaacgacca ggtccggttc gacgtgacgt ttcagacgct tgcccccgat
      661 ctcaagatca tcgcgccggt gcgcgagtgg aagtggacgc gaacccagga gctcgagttc
      721 gccgccaagc acggcatcga ggtcgaggcg acgaaaaagt cgatcttcag caccgaccaa
      781 aacctctggg gccggtcgat cgaggccggc attctcgaag atccctgggt cgagcccccg
      841 cccgagacct tccagtggac gaccgacccg cgtctggcac ccgacctacc ggcggagatc
      901 gagatccgtt tcgatcgcgg caagcccgtg gccctggacg gccgggagct cgacggagtc
      961 gagttgatcg gcgagctcaa tcagcttgcc ggcaagcatg gtgtcggccg gatcgaccat
     1021 atagaaaacc gcttggtcgg tatcaagtcg cgtgaaatct acgaggcccc ggccgccatc
     1081 ttgctccacg aagcccaccg ggaggtcgag tttttgaccc tttccaagga agcgctccgg
     1141 ttcaagacgc aggtcagcca ggcctatgcc gatttgatct ataacgggct ctggttcagc
     1201 cagctccacc aagacctgat ggccttcacg gtctcgaacc agcaatacgt gtcggggacc
     1261 gcccggatta agctctacaa agggcacgca cacaccgtgg gccgcaaaag cgaccatagt
     1321 ctttaccgtc acgagctggc gacctacgag gaaggggatc agtacgactc gtctgccgcc
     1381 ctcgggttca tcaagatcca cggactgggc cagacgacgc aggccaagca ccagcttttg
     1441 cgtggccccg gcggccggcc gctcgagctc cccagcatca ttccgccgtc acgggctgaa
     1501 tcccctgaca agagctaggc ccggatgacg cggctgggca cctcgcgcgt tagcgcacct
     1561 aaccggggct ttgacaaccg tttctccttt ggtaacatgt tagattcgac ctggatcgcc
     1621 ggggcgaccg gtgcacgccc ggccactctt aatccgaacg tcttcgcatg gatcccgcag
     1681 gcggggagtg aaggattcgc cgtggttcga gggctggacg ggcagcccga cgggctgcgg
     1741 cgtgaagtcg agccaggcag agtcagggtc aggagataat ttaagcatgc cggagatgat
     1801 gaccaccgac gttcgagcga tcatggaccg ctctccgttc gacgtcgccg cggtggcgga
     1861 cctgcgtgag ctcttgaatc gcgatccgtc tcgttaccga acgctgcgcg aggccgtggc
     1921 caatatcaag gaacgcgaga agaaagaaaa agagtccaag ccggagacgc acttgcgtct
     1981 gggtgtcggc gaggtgttgc tgggtcgtta caattcgggc cttgagcact tgaagaaagc
     2041 cggcgacctg ggaatggcgt gttttttccg gggtctcgcg cttgagaatc agcagcggta
     2101 cgaccaggcg gccgccgcgt ttgcccaggc ggccaagcag ggttatgacg ccaagaccgc
     2161 cgatctgcac cgcgccggcg ccctccgccg cgccggcaat atcgaagagg ccaagaagat
     2221 cctcgccagc ctgcaaaaac acggcgaatc gtcccccgaa tatcacttcc agcaaggctg
     2281 cgtgctcgcc gcctcgggcg aactggtcga agcatcggct cagtttgaaa agacgctgag
     2341 catcgaaaaa gaccataacg gcgcgctctt cgagcttgct tatatcaacg atctctatgg
     2401 caacgacgat ctggctctgg aatactaccg ccggtgcaca gatcgccccc cagtgccctt
     2461 ggcagcgtgg atcaacctcg gcatcctcta cgaggatgag atgaggtttc gagacgccga
     2521 gcagtgctac cgccacgtgc tggctcacga gcccaatcat ccccgcgccc ggctgtttgt
     2581 caaagactgc caggccagca aggggatgta ttacgacgag gaagccgagc gcggctatac
     2641 ggtcctcaag cagttgcacg aaattccggt caccgacttt gagctctcgg tccggagccg
     2701 caactgcctg cgcaagatga atattcgtac gctgggagac ctgacacgca ctaccgaggt
     2761 cgccctcctg tcgagcaaga attttggtga gacttcgctc gccgaaatca aagacatgat
     2821 gcaggccaaa ggtctgcgtc tggggatgtc gctggaaggc aacgaacgcg ccggccacga
     2881 tcaccgggtc gagcaaccgc aagaagttcc ccccgagctg caggcgatca tgaacaagcc
     2941 gatcggggac ttgaacctgt cggttcgcgc ccgcaagtgc atgagcaagc ttaacatcca
     3001 gacggtcggc gacctgctca agcattccgg cgacgaactt ctcgaatgca agaacttcgg
     3061 cgtgacgtcg ctcaccgagg ttcgtgaaaa gctcacctct ctgagcttga aactgaagaa
     3121 cgattgagcg gacatgcgca ttgcgacagg agcgtcgacc gcgtcgtcta cccccatccg
     3181 gtttacgagc caggctgttg attcgggttc tggggcgcgg cttgggctct tggaaacgcg
     3241 gagcggatct ctcgagacgc cggccttcat gcccgtggga actcaggcca cggtcaaggg
     3301 tctcaccccc gagcagcttc gaaccagcgg cacccggatg ctgctggcga acacctatca
     3361 tcttgcgctt cggcccggcg ctgaggtggt gcggtcattg ggcggtctgc acacgttcat
     3421 gggctgggac ggtccgatcc tcaccgattc gggtgggttt caagtgttca gcctggccga
     3481 ccgcactcgc gtgaccgacc acggcgtcac ctttcgatcg catctcgacg gtcggctctt
     3541 ggagctgaca cccgagagtg ccacggccat ccaagaggct ctcggggccg atgtagccat
     3601 gtgcctggac cattgcccct ctctgccggc gtcgacggct cagattgccg acgcggtcgg
     3661 ccgcacagtc gattgggcgc ggcggtgcaa ggccgcacat gagcgccggg atcaggcact
     3721 ctttgggatc gtgcagggcg ggccgcatgc agacctgcgc gcccaatgcg ccagcgagct
     3781 tctcgccctg gatttcgacg gttatgccgt cggcggcgtg agcgtcgggg aagatcgcca
     3841 gacggtgcgc caggcgctcg ccgtgaccac ccaccttttg ccggccaatc ggcctcgcta
     3901 tctgatgggc gtcggccgcc cgcaagatat cctcgacgcc gtcgccacca ggatcgatct
     3961 cttcgactgc gtgatgccaa ctcgcaacgg tcgcaatgca acttgtttta cgcaaaccgg
     4021 atatgtcaaa cttcgaaacg ccgtgcatcg gcttgatccc cgaccgcttg aggaaggttg
     4081 tgactgcttg gcctgccgcc ggttcagccg ctcttatctg cggcacctgt ttctggccaa
     4141 ggagatgctc ggcccaatct tggcgtcgat ccacaacctg acgtttcttc atcgcctgac
     4201 ttcccggatc cgcgcagcga ttgcggcggg tcggtttgtc cagttccgtc tagaagtcct
     4261 tgaagcatta ggaccctaag cgttagagtg caatcgaagg gtcaagtcgc tgcacgatgc
     4321 tcaactgtaa ccacggcgac gaccggctcg gccacacgtc gaccgggctc ttcgcgccga
     4381 tcgccctgat atagaatgga ttttcccacc atgattggat tattggccga gattcccctg
     4441 ctgtttgccc aggacgcggc gagcgggagc atctggtcgc tcctcttgcc gctggcgccg
     4501 attccgtttt tgtattactt cctggtcttc atgccccagc agcagcagga aaagaagcgc
     4561 cgcgcgatga tcgacggcct gaaaaagaac gacaaggtcc tcaccaatgg tgggatctac
     4621 gggaccgtga tgtctgttga ctccaccaac gacaaagtgg tgttgagggt cgacgacgaa
     4681 aaaggagtaa ggatggcgtt caccaagtct agtgtggcac gcgtcctgga gctatctgcg
     4741 gaaaagtcaa cggaatcgac ctgatatccg cttttgcaca gacaaccgca caccgcattt
     4801 cttgatatga tcgttgcaat cgccccccag aggcccctcg tgcgatccct gatagccggc
     4861 cgaagcgagc agctggcatc gataaagagg ggctggagaa agaacaatga aacggtttgg
     4921 atggaagatc ggcttgatcg tcggcagcac ccttctcggc ctggccttca tgtggccgcc
     4981 atcgcagaag ctgaagctgg gtatcgacct ctcgggtggg acgattctgg tttatgaagc
     5041 cgagaatctg cctcccggct tcaacctgga cgaattgatc gccgcgctca agaagcgtgc
     5101 cgatccggaa ggggtcaagg aaatccccat ccgcaagatc ggcggcaatc gcatcgagat
     5161 catcctgccg caggcgagca acgaggaggt cgaagaagtc aagaagatgc tgacggatgt
     5221 cggctcgctc gagttccgca tcttggccaa tggcaagcac gacaaggagg tcgtgggccg
     5281 ggcactcggg gccattttgt ccaagccgcc gtcgcgctat atgtgggccc gcctgggcga
     5341 aatctcaaat ggaaccaacc ccaccttcac gagcgactcg atcaccgatc cggaccagtc
     5401 gtggaagaaa gatctctacg ctggaaccga tgtctatgtg accggcaaag actcgtcagg
     5461 caactcacgg accgaatcgg tcaaggtgcg ccgcaatacg accaacacat tgatcttgga
     5521 caaaccgcat ggcctcaaga ccgtcacgtc ttatcggatc gaatacaatc ccagccatct
     5581 gaaggggggc gaccccaaca acctcgggcc caatgacctg atcgtgcgcg aggagaaagt
     5641 cgctcccggc cgcgtcgaga cctatatcct ggccaacctc gatcggccag ggcaggaagt
     5701 cacggggaag tatttgcggc aagcaaagcc cgatgtggac gaccggctcc agccggccgt
     5761 gcgattcgag tttgattcgc gcggagcaag ccgctttggc aaactcacac gcgaacacaa
     5821 gcccgaagag ggtggcgcgt tccgctatca attggcgatt ttgctcgaca agctggtgat
     5881 gtcggcgcct gcgatcaact ccgagatccg cgactcgggg atcatcgagg gagggccgca
     5941 gggcttcaag gccaaggagg tcgagcacct gatccaggtg ctccgcgctg gcagcttgcc
     6001 cgcgagcttg aacccgaacc cgctgcagga agaaaaggtc ggacccactc tgggcgaaga
     6061 cacaatcgcc aagggtgtgt ttgcgatctg ggtatcgctg ttggtagtgc ccatcttcat
     6121 gatcatctat taccgcttcg ccggtgtcgt ggcggtcctt gccttgctcg taaacatgat
     6181 cctcttgatc gggtcgatgt cgttcatcca ggccaccttt tcgcttcccg gcctggctgg
     6241 cctcgcgctc acgatcggta tggcggtcga cgccaacgtg ctggtgttcg agcgcatgcg
     6301 cgaggaaaaa gaacgcgggg ccagcctggc ccaacagatc cgcaacggat tcaaccgagc
     6361 ctgggtcacg atcttcgact cgcacgtcac cctcttcctt gccgccctcg tgctgtacat
     6421 ggttggcacc gaggaagtca aagggttcgc gctcacgatg atcatcggca tggcctggaa
     6481 cctgttcacg gccgttttca tgtcgcgtgt catctttgac tatttctata gccgcggatg
     6541 gctcaagcaa gtgaacatgc tcaagttgat ggacaagacc aacttcgact tcatcggccc
     6601 gcgctattac tgcatggctg gctcggtgat cctgatcgtc ctggggctga tcgcgacggg
     6661 tgttcgtggt caatcgatgt tcaacatcga cttcacggga ggaaccctgg tcacgatccg
     6721 cttgaatgaa agcgacccca gcgtcaaacc cttgaccgaa tctcaacgag cgcaactggt
     6781 tcgcgaaaag gccagcgtct tgccggacgt gaccgtcgag agcctgcggg tgggaaacga
     6841 caaaaacctg gcgaggttta acatccgcac gaccgaacag gaccagagca aggtcaagcg
     6901 cgtcgtcctc gaggcgttcg ggtccagcct ggcgcgggtc gagatgaact acggcgaggc
     6961 caaaccgatc gcggcggcga ctccgccggc cgccgaagct ggtaaggccg caccgcccgt
     7021 ggtgaaccgt ttcgccggtg gccgccagta cgagctcacg ttcaacacaa ccgcatttaa
     7081 cagcgagcaa tcccccgcac aggttgtctc ggccgtcttc gcgcgggtgc tcgacaacgc
     7141 caagatcgcc aatcccatct cgcggtttga gttgatcccg ggccccgatc gccccgccgc
     7201 ggccacggcc ggcggcgcgt cgacggcacc agcggcggga accaggctta ttctccgcac
     7261 cgacctggag cctgaggtgg ccaaggtcga gctcgagcag ctcaagaagg ctttggctga
     7321 tgaccccgat ctcttgttcg agcgaagcat gaacttcggc ggcaccgtcg ccggcgagac
     7381 ccgccagcgc gccttggtcg ccacgttcgc aagctggctc atcatcatcg cgtatctctg
     7441 gtggcgtttt cactcgttta cctatgggtt tgccgccgtg ctggccgtgg tgcacgacgt
     7501 gttgatcgcc ttgggcgcaa tcgccgtctc ctactggctg gccaaggtgc ccggcctgtc
     7561 aactctgctg cagatcgatc aattcaagat cgacctgcca atcgtggcag ccttcctgac
     7621 cttgatcggc ttttcggtca acgacacgat cgtgatcttt gaccgtatcc gcgagatcaa
     7681 gggcaaaacg cctcacctga ccaacaagat ggtcaacgac gcgatcaacc agacgttgag
     7741 ccgaaccatc ctgacatcgt tcacggcctg gctcgtcgtc gtgatcctct actttttcgg
     7801 ctgcgaaggc ctacatggct ttgcgttcgc actcgttgtc ggcttcttga gtggcaccta
     7861 cagcacggtc tatatcgcca cgccgatctt gatcgacttt gtcgcgacca agcccgggcc
     7921 gcccaccaag gtcggtaaac agttggcagc ggccaggtca ctattggatt aagaccgaat
     7981 tggccgaaag cagatcactt tccccccctt gtgaaggggg gggcaggggg ggtggtcagt
     8041 gagcaatcag gcacagttct tccagagatt taattagtaa atcaggctgc tttcactcat
     8101 tagctatgaa tattatgtaa ttgatagcac cgggaccacc tcaattcaca ttgaccacac
     8161 tgtgtctggt agctcgctga ccaccccccc aacccccctt cacaaggggg ggaaagctgc
     8221 gttgatcttg attgctttat gatgccgctc agatcagcgt ttccagcaag agtttcagct
     8281 tgcgcaactc gacttcgcga tcgaaggggt agtcgggctg gt
//
LOCUS       DATAQL010001418         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_146637, whole genome shotgun sequence.
ACCESSION   DATAQL010001418 DATAQL010000000
VERSION     DATAQL010001418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146637"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VHS97_24525"
     CDS             complement(<1..194)
                     /locus_tag="VHS97_24525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451442.1"
                     /translation="MSGRRLILRASLAASLIVNAGCRSRENPDIAARAPQGSPTGGLA
                     RASEPKPSEAVANGNPENAAG"
     gene            336..455
                     /locus_tag="VHS97_24530"
     CDS             336..455
                     /locus_tag="VHS97_24530"
                     /inference="COORDINATES: protein motif:HMM:NF028380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEX3451443.1"
                     /translation="MGKGDRRSKRGKIFRGTYGKKRPKPQEERKKAKAAAAQS"
     gene            685..912
                     /locus_tag="VHS97_24535"
     CDS             685..912
                     /locus_tag="VHS97_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451444.1"
                     /translation="MAKLRLLKTGHGDLTLAEWDKDRPETVATAEAAFAEHCTAGRLA
                     FRLDGPGRSTPIRQFEKSASEILIVPAIQGG"
     gene            1045..1908
                     /locus_tag="VHS97_24540"
     CDS             1045..1908
                     /locus_tag="VHS97_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451445.1"
                     /translation="MIRTGYLRWEIEVQDVRSQDDPQSREVIFLARPSYRVIRVKRTS
                     HADAAERFVRGEGTYDSVLRTLDRDGGETDVRVPNIGPRPFRYTESDRAGLPRYWMEK
                     LQREVSAFAHEVVRTAASFPQLVISSPALRAGYARLVTWEIFRVIEGKSHPGATEENE
                     PMRLDASSYQEGVPELVAAELVRNVVEPKEFHEFCEFDCLTVLNGDKYYRIPRRPHAL
                     IEVWDAKTRRALSRLCVVFQDPGMPPSDEVVMKYLLAKHQPDLLWKVGIRFSPPTGKF
                     EVTPPRRWTLD"
     gene            complement(1944..3200)
                     /locus_tag="VHS97_24545"
     CDS             complement(1944..3200)
                     /locus_tag="VHS97_24545"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3451446.1"
                     /translation="MRLRRFGSKIASAVLVYGWAWLALAGCAQEEPLEPDPGASPFYP
                     GPSVANGAAAKAEKTAQSGLNQSDAKAAADDSAAAGSPGTDSIDTRFGTNDVEKRLRS
                     ALRTAPKDPVAAAEQLDKLLAIEPIHREALYGRATLALEQSRSANSPEERTAHVDKAV
                     QSVRALTRAYDNIKPHEKELFARILYRNTQVLAEKGHVDQAVAVVKEASELGFDAFGK
                     VESDSSMAPLRATSQFQAAQKADDDLKLAQSRGRVKSILEKPLNARFDFTLPDLSGKK
                     QSLQDFKGRVVVLDFWGTWCGPCRETIPRLIELYKRRHASGLEIVGLSYERDATSESQ
                     AAELVKEFVKSNGVPYPCLLGDEATIKQVPGFRGFPTTVIVDRAGKVRLIITENSAQT
                     PEVITDVVRVLLAEPVPDGAAAAKKS"
BASE COUNT          628 a          973 c          989 g          641 t
ORIGIN      
        1 ccggcggcat tttccggatt cccgtttgcc accgcctccg aaggtttcgg ctccgacgcg
       61 cgggcaagcc cgccggtagg cgaaccctgc ggcgcacgtg cggcgatgtc agggttttcg
      121 cggctccgac agcccgcatt aacgatcaaa ctggcggcca gacttgctcg caaaatcaaa
      181 cgacgaccgg acatcgaaat cactcctgcc gtcgagatcg atgacgcgca gttaaaacga
      241 caaacgtcag tgatttgatt catcctaatc ggataggctc ggaaactcaa gctgcttact
      301 gaaatcgtgt gccatgtttt ctggggagga cagtgatggg taaaggtgac cgacggtcga
      361 agcgaggtaa gatcttccgg gggacatacg ggaagaagcg tcccaaacct caggaagaac
      421 gcaaaaaggc caaggcagcg gctgcccagt cgtagcccgc cctggccgcc gcgaaccgcg
      481 gcgaactccg ctgtcaagag gcaaaatgac gtggaaccac gttcgcagtg gctgcgattc
      541 gggcggttga ttagaatcat cggtccgcgc cgtttgaaag cagttgtgcc tcgagcaggc
      601 gcgttttgtc ggaagattct atagtctggg cgatggactt aactggacca gcgaatggat
      661 cgaggtggaa ggagagctcc agcgatggca aagcttcggc tgctcaagac cgggcacggc
      721 gacctgaccc tagccgagtg ggacaaagat cgacccgaga cggtcgccac cgcggaagcc
      781 gcgttcgccg agcattgcac ggcggggcgc ctggcttttc gactcgacgg accgggtcgt
      841 tcgaccccga ttcgtcagtt tgaaaaatcg gcgtcggaaa tcttgattgt cccggcgatt
      901 caaggaggct gatgcccaac acctgttcgc gccgtcatgg ccgatttagc ggccgccgcc
      961 acggccgcgc acaaacggct ggacagacgc gctactggcg gcagcgactt tcaactatgt
     1021 tcaggccgtc tggtgagcgt ggttatgatc cgaacgggat acctacgctg ggaaatcgaa
     1081 gtccaggacg ttcgcagcca ggacgacccg caatcgcgcg aggtcatctt ccttgcccgg
     1141 cccagctacc gggtgatccg cgtcaagcgg accagccatg ccgacgcggc ggaacggttc
     1201 gtccggggcg aggggaccta cgactcggtc ttgcgaacac tcgatcgcga cggcggcgag
     1261 accgacgtta gggtcccaaa tattggcccc cggccgttcc gctacaccga aagcgatcgg
     1321 gccgggctcc cgcgttactg gatggaaaaa ttgcagcgtg aggtcagtgc cttcgcccac
     1381 gaagtcgtac gcacggccgc ttcgttcccc cagttggtca tctcgagtcc ggctctccgc
     1441 gccgggtatg cgcggctggt cacgtgggag atcttccgtg tgatcgaggg caagagccat
     1501 cccggtgcca ctgaggaaaa tgagccaatg cggctcgacg cgtcgtccta tcaggagggc
     1561 gtgcccgaac tggtcgctgc cgagctcgtt cgcaatgttg tggaacccaa ggagttccat
     1621 gagttttgcg agttcgattg cctgaccgtc ttgaatggcg acaaatacta ccgcatcccg
     1681 cggcggcccc acgccctgat cgaagtttgg gacgccaaga cccgccgcgc cttgagccgg
     1741 ctctgcgtgg tctttcagga cccgggaatg cccccgtccg atgaggtcgt catgaaatat
     1801 ctactcgcca agcaccagcc ggatctactc tggaaggtgg ggatccgctt cagtccgccg
     1861 accggcaagt tcgaggtcac tcccccgcgg cgctggactc tcgactagac gcgatcattg
     1921 cggccatcgc agcctgtcac cgatcaagac ttcttggctg cggcggctcc gtcgggaacc
     1981 ggctctgcga gcagtacgcg gaccacgtca gtgatcacct caggtgtctg tgccgaattc
     2041 tcggtgatga ttagccgcac cttgcctgct cggtcgacaa tcaccgttgt ggggaagccg
     2101 cggaatccag gcacctgctt gatcgtcgct tcatcgccga gcagacaggg atagggaacg
     2161 ccgttactct ttacgaattc cttgaccaac tcggctgcct gcgactcgct cgtggcgtcc
     2221 ctttcataac tgagcccgac gatttccaga ccactcgcgt ggcgcctctt gtagagctcg
     2281 atcaggcgcg gaatcgtctc gcggcagggg ccgcaccagg tcccccaaaa gtcgagcacg
     2341 acgactctcc ccttgaagtc ttggagcgac tgctttttgc cgctcaagtc gggcagggtg
     2401 aagtcgaaac gggcgttcaa gggcttttcg agtatactct tcacgcgccc gcgagactgg
     2461 gcgagcttca aatcatcgtc ggctttctga gccgcctgaa attgagacgt cgctcgcagc
     2521 ggtgccatcg acgaatcgga ctcgaccttg ccgaaggcgt cgaaacccag ctccgacgct
     2581 tccttgacga cggccacagc ctggtcgaca tgaccttttt cggccaagac ttgtgtgttt
     2641 cgatagagga ttcgagcgaa caattccttt tcgtgaggtt tgatattgtc ataagcgcgt
     2701 gtgagcgcgc gcacggactg aacggctttg tcaacatgtg cggtacgctc ttctggcgaa
     2761 ttcgcgctgc gcgattgttc gagagccaag gtcgctctgc catagagtgc ctcgcgatgg
     2821 attggctcga tggcgaggag cttgtcgagc tgctctgccg cggccacggg atccttcggt
     2881 gccgtccgca gtgcgctccg taggcgtttt tcgacgtcgt tggtgccaaa tcgcgtatcg
     2941 atggagtcag tcccaggcga gccggctgcg gccgaatcgt cggcggccgc tttcgcgtca
     3001 gactggttca ggccgctttg ggcggtcttt tccgctttgg cggccgctcc atttgcgacg
     3061 gacgggcccg gataaaaggg cgatgcgcca ggatcgggct cgagcggctc ttcttgagca
     3121 cacccagcca gagccagcca ggcccaacca tataccaaga ccgcagaggc aatcttcgac
     3181 ccgaatcgac gcaaacgcat gagttactcc ggtcaactgt ttttgtcgaa g
//
LOCUS       DATAQL010001419         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4419034, whole genome shotgun sequence.
ACCESSION   DATAQL010001419 DATAQL010000000
VERSION     DATAQL010001419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4419034"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1195)
                     /locus_tag="VHS97_24550"
     CDS             complement(20..1195)
                     /locus_tag="VHS97_24550"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEX3451447.1"
                     /translation="MRDVALLARLLALPNPWRVERVGFDPDEQRVDLWLVHRRNATFT
                     CPECGARSPLHDHLPWRTWRHLDHGECVTWLHARPPRVDCPAHGIHQVRLPWALPGCR
                     FTIPFERRAIDVLLQADVLGGAGLLRISWDEAWHLMERAVARGQRAKRRRVMARLGVD
                     EKAASKGHRYVTLVFDLDRSTVEYIADDRKKASLDAFYLSLSEEQLTGIEAVAMDMWE
                     PFVASTAEHVPDGRSKIVFDRFHVMKHMTEAVDAVRKWEHRRLQAEGDDTLKKTKYLW
                     LYSEENLQESSRERFAALRSLHLKTGRAWAIKESLRDLWEYRRRGWALRHWRHWYFWA
                     THSRLKPVIKVARMIRDRLDNVLTYLEHRITTATCEGFNSKIQTIKKTPMASAIGTI"
     gene            complement(1449..>3521)
                     /locus_tag="VHS97_24555"
     CDS             complement(1449..>3521)
                     /locus_tag="VHS97_24555"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX3451448.1"
                     /translation="EATGLEVDKLSKQFVKAYYDYYLDKFKEATGGLMGKRGLHYLLH
                     DNFEVGQGNWTNEMMAEFKRRRGYDMTPWLPTLVGHVVESAEASDRFLWDFRKTIAEM
                     TAEYNYDMLSDMLKERGMGRKTEGQEFTRVYVVDGMDVKRSAAIPMGAAWAGNPWETS
                     HKEDLREAASVAHIYGQNLVGAESFPAFAGGEGDGGGRSSLYAFAPSMLKPTADAALA
                     DGVNRFEIYSFAHQPVDDKAPGIAEAYGQWFTRHETWAEYAKPWMTYLARSSYMLRQG
                     HFVADILYYYGEDSNITALFGERGPDVPEGYALDYASSDVVLNRIGVTNGRFTTTTGM
                     SYRVLVVDDNAVHMPLNVLKKIRDLVNAGAIVIGPKPTDSPSNSDNQAEFKTIADQLW
                     GSGEGARKVGKGQVFAGQSAAAVLKALQISPDFEYTKPQSDTSVAFVHRKISDGDIYY
                     VYNRKNRAENVEATFRLAGKAPELWHAETGEIEPASYRVANGRTTVSLKLDPAEAVFI
                     VFRKAGSAQGRTLPQVTETAVGTIDGSWTVAFQPNRGAPASVTLDKLSAWNDSADPGV
                     KYFSGTATYTKTIEAPAEWFATGAKMLLDLGSVKNIAEVTVNGKSAGVLWKPPFRADV
                     TGALKPGANTVEIKVTNLWANRLIGDQQPNVTKKYAWTSLDAPGADSPLLPSGLLGPV
                     RVLRTSSN"
BASE COUNT          626 a         1125 c         1091 g          679 t
ORIGIN      
        1 tgaaagaaga tggccgtctt cagatggtcc cgattgcgga agccatagga gtttttttaa
       61 tcgtctgaat cttcgagttg aacccctcgc aggtggccgt ggtgatccga tgctccagat
      121 atgtcaagac gttgtcgagg cggtcacgga tcatccgggc gaccttgatg accggcttca
      181 gccgcgaatg cgtcgcccag aagtaccagt gccgccaatg ccgcagcgcc cagccgcggc
      241 gccggtactc ccacaggtcg cgcaacgact ccttgatcgc ccaggcgcgg ccggtcttca
      301 agtgcagcga ccgcagggcc gcgaaccgct cacggctcga ctcctgcagg ttctcctccg
      361 agtacagcca caggtatttc gtcttcttca gcgtgtcgtc cccctccgcc tgcaaccggc
      421 ggtgctccca cttccgcacc gcgtcgaccg cctccgtcat gtgcttcatc acatggaagc
      481 gatcgaagac gatcttggat cggccgtcgg ggacatgctc ggccgtcgag gcgacgaagg
      541 gctcccacat gtccatggcg acggcctcga tccccgtcaa ttgctcctcg gacagggaca
      601 aatagaaggc atccaaactg gccttcttgc ggtcatcggc gatgtattcg accgtcgacc
      661 ggtccaggtc gaagaccagc gtgacgtagc ggtgcccctt ggaagccgct ttctcgtcca
      721 cgccgagccg ggccatgacg cggcgccgct tggcccgctg gccccgggcc accgcccgct
      781 ccatcaggtg ccacgcttcg tcccaactga tccgcagcag cccggcgccg cccaggacat
      841 ccgcctggag caagacgtcg atggcgcggc gttcgaaggg gatggtgaac cggcagccgg
      901 gcaaggccca ggggagacgg acctggtgga tgccatgggc ggggcagtcg acccggggcg
      961 ggcgggcatg caaccaggtc acgcattcgc cgtgatcgag atgccgccac gtccgccacg
     1021 gcaggtgatc atgcaggggc gatcgggcgc cgcattccgg gcaagtgaag gtggcattgc
     1081 ggcgatgcac cagccagaga tcgacccgct gctcatccgg gtcgaagccg acgcgttcga
     1141 ccctccaggg gttgggaaga gccaggagtc gagcgaggag agcgacgtcg cgcatggccg
     1201 ggacctcgtg gaaggcgaca gggccccgaa tcgggatggt cttggtgcat tgtccgacgg
     1261 gacagagaag aggggaaggg ggtgggggtc gggacctggg ggtcctgctg gtcccgcccc
     1321 ccaagccccc gcgacggaaa acgcggccgc aatcccccaa gccttgtaca cgactggctc
     1381 tgaccgctgg atttgtaccc acacgaaacc cggaagaacc gaaattgata tgcccggcta
     1441 tggcttcgct agttactcga cgttctcagg acccgcaccg gcccaagaag gccggaggga
     1501 aggaggggtg aatcggcgcc tggagcgtct aatgacgtcc acgcgtactt cttcgtgacg
     1561 ttgggctgct ggtcgcctat caaccggttg gcccacaggt tcgtcacctt gatctccacc
     1621 gtattggcgc cgggcttcag cgcgccggtg acatcagccc ggaacggcgg tttccacagc
     1681 acgccggcgg acttgccgtt caccgtgacc tcggcaatgt tctttacgct cccgagatcc
     1741 agcagcatct tggcgccggt cgcgaaccat tcggccgggg cctcaatggt cttcgtataa
     1801 gttgccgtgc cggagaagta cttcactccg ggatcggcac tatcgttcca cgcgctcaat
     1861 ttatcgagcg tgacgctggc cggagcgccc cggttgggct ggaacgcgac cgtccaggaa
     1921 ccatcgatcg tcccgaccgc cgtctccgtg acctgcggca gcgtgcggcc ctgcgcagac
     1981 ccggccttgc gaaagactat gaatacggcc tcagccggat cgagcttcaa agaaacggta
     2041 gtgcgtccat tggcgactct gtatgacgcc ggctcgatct cgccggtctc ggcatgccaa
     2101 agttccggcg ccttgccggc caaccggaaa gtggcttcca cgttctccgc gcggttcttg
     2161 cggttgtaca cgtagtagat atcgccatcg gaaatcttgc gatggacgaa agccacggac
     2221 gtgtcgcttt gaggtttcgt atactcgaaa tccggagaga tctgcaaagc cttgagaacg
     2281 gcggctgcgg actggccagc gaatacctgg ccctttccga ccttgcgcgc gccctcgccg
     2341 gaaccccaaa gctgatcggc gatggttttg aattcggctt gattgtcgct gttgctaggg
     2401 ctgtccgtcg gcttcggacc gatcacgatc gcgccggcgt tcaccaggtc gcgaatcttc
     2461 ttcagcacgt tgagcggcat gtgaaccgcg ttgtcatcga cgaccagcac gcgatagctc
     2521 atgccggtgg tcgtcgtgaa gcggccgttc gtaacgccaa tgcggttcag gaccacgtcc
     2581 gagctggcgt agtcgagcgc gtagccctcc ggcacatccg gccccctctc gccgaagagc
     2641 gccgtgatgt tggaatcttc gccgtagtag tacaggatgt cggctacgaa gtggccctgc
     2701 cggagcatat agctgctgcg cgccagatag gtcatccacg gcttggcgta ctcggcccag
     2761 gtttcgtgac gagtgaacca ctgaccgtaa gcctcggcaa ttcctggagc tttgtcgtcc
     2821 accggctgat gggcgaagct gtaaatctcg aaccggttta ccccgtccgc gagtgcggcg
     2881 tccgcggtcg gtttgagcat cgagggcgcg aaggcgtaca agctgcttcg cccaccgcca
     2941 tcaccctcac cgcctgcgaa ggcgggaaat gactcagccc caaccaggtt ctgcccatag
     3001 atgtgggcca cggaggcggc ttcgcggagg tcctccttgt gagatgtctc ccacgggttg
     3061 ccggcccacg ccgcgcccat cgggatggcg gcgctccgct ttacgtccat gccgtctaca
     3121 acgtagacgc gggtgaattc ttgcccttca gtcttgcggc ccatgccgcg ctctttcagc
     3181 atgtccgaga gcatgtcata attgtactcg gccgtcattt cggcgatggt tttgcggaag
     3241 tcccagagga accggtcgct ggcttcggcg ctctccacca catgaccgac cagcgtcggg
     3301 agccaaggcg tcatgtcgta gccgcggcgc ctcttgaact ccgccatcat ttcgttggtc
     3361 cagttcccct ggccgacttc aaaattgtcg tgaagcaggt agtgcaggcc gcgtttgccc
     3421 atcagcccgc cggtagcttc cttaaattta tccaggtagt agtcgtaata ggccttcacg
     3481 aactgcttgc tgagcttgtc cacttcgagg ccggtcgcct c
//
LOCUS       DATAQL010001420         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2743316, whole genome shotgun sequence.
ACCESSION   DATAQL010001420 DATAQL010000000
VERSION     DATAQL010001420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2743316"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1062)
                     /locus_tag="VHS97_24560"
                     /pseudo
     CDS             complement(1..1062)
                     /locus_tag="VHS97_24560"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1120..>3741)
                     /locus_tag="VHS97_24565"
     CDS             complement(1120..>3741)
                     /locus_tag="VHS97_24565"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX3451449.1"
                     /translation="AGEFILATSSATGNERISVLRTGATVGASLTLVGSINFSFPTNW
                     EHTSFASVVRPTPGQSGNFDVIFNIGAQYNGVVIGGNGSVVLDNKGNPTYEPSTGTVS
                     ASGLISGTLVGDSLYMVTLHDDNHNPVVSNLTRISSGLRNAASLAIDPTSGDLYLADN
                     GIDGNNGGNEAWSADELNRIPKAQIGVTVPFFGFPEVINGQLTYSYVKTIDKPGEPVT
                     VVNSGVGVQPLVAFQPLPDPVLTAEGSESEGSSGFALSPSGFPAGLNHGAFIGFHGVF
                     DAGGTANDENPLVFANPSTGHYFDFVSNNESGIGHIDDILSTPDSLYLADIASGGSMG
                     SGAGQGVIYQIKAITNQVVNHPPVLGSIADQTVDEGTKLTVQASATDPDPSQTITYSL
                     GPGAPAGASIGAQSGTFTWTPNPYSSTGTYSITVVATDNGSPPLSDSIAFTANVLAVN
                     HPPNLQNIPTQVVEQGQPLQVKVRDFVSDPDIPAQALSYSLAPGAPSGASIDATGLFT
                     WTPQSNQPINTYTIGVVVTDNGSPPLSGSKTFTVNVVPFNHPPVLSTIPTQNVDEGNP
                     LTIDLTATDSDVPAQSINYTLGAGTPAGAAIDSQSHRFIWTPNAYSGSGTYSITIIAT
                     DNGYVPKSDSTTFTVNVLPVNHPPVIAAIPTQTAVLKQKLQLNLARYTSDPDTPAQML
                     AYSLVPGAPSGVIVDPVSGLLTWTLSSRQRIGTFSIGVVVSDSGSPQLSQTTSVTVNV
                     FDVGPAATIARASVSTKNGLAITLKFSQPLDPSTAENASNYVLVPIKLKNSKTPTIVT
                     PIPLSANYNASTSTVTLTSTVRVKARQSFKLTVIGQGPDGIANVSGLLMAGVRKRAGT
                     NYVASITGKSIRQISKS"
BASE COUNT          653 a         1072 c         1268 g          748 t
ORIGIN      
        1 cgggctcgag attggtcttc agctccacgt tcgcccgcgc agccagcgcg tgtttggcgc
       61 ggccggccag agcacgcact cgggcttcga cctctcggat gccgatcagg tcgagcaggt
      121 ccaccgcggc ttccatggcc accacccgtg gatcatcctg ctggcccacg gcctcgaact
      181 tccgggcgcc cttgagttcg tcgctccagc ccgccgaaac gatcgaaggc cagagctgct
      241 cgatccgctc ggcgcggacg aagaggatcc cggcttccag cgggcccatc ggccacttgt
      301 gcgcgctcgc tgagtaggag tcgcacccga gatccttgag attcacatcg cccgcgccga
      361 aggtctggcc ccgtccacgt gcaggaagat gtcgcgccgt cgggccagct cggcgatcgg
      421 tttgatgggg tatcggatgc cggtcgtgct ggtcaggtgg gtgaccgcga tcacgcgggt
      481 gcgcggcgtg agggctttct ctatgccggc gaccaggtcc tcggcagaac gcgccgggac
      541 ggagaccggc acactcttga ccaccagggc ctcgcgccga ccgcgcagcc tccagctgtc
      601 gttgttcgag ggatggttgt ggtcggtaat gatgacctca tcgccgggct tcaggtcgat
      661 cccccggacg acgaggttgg tgccttcact ggtgttgcgg gtgatggcga cctcgtcggc
      721 ggagacgccg aggagcgcgg ccaccttttt gcggaccgaa tcccgaagcg ggcggtactt
      781 gtctcgattc tggaacgacg ggttggcctg gaagtcgcgg agcagcgcgg cgtaccgatc
      841 cagcaccggc cgcgaggcgg ggcagacgtt ggccgcgttc aagtagatga gcccatcctc
      901 gagcgggaac tgccgcctga cgagttgcca cagcgcctcc tcggagcggc cgctcgctga
      961 ggctcgggcc gtacctgcgg ccgcgagcgc cgcctcgaac cccaaccagc tcgccggcag
     1021 ggtcgccgag cgatgcagga actggcgtcg ggtcggtttc atggctgact ccctctctcg
     1081 ttccgctgga agaaaaacgc gacgcgcaga ttgcgatgct cacgacttgc taatctgcct
     1141 gatgctcttg ccggtgatgc tcgccacgta gtttgtgccg gctcgtttgc ggacgccggc
     1201 catcagcaaa ccgctcacat tggcgatgcc gtcgggtccc tggccgatga ctgtcagctt
     1261 gaaggactgc cttgccttga ctcgtacggt tgaagtgagc gtgacggtgc tggtactggc
     1321 gttgtagttc gcgctcaatg ggattggcgt cacgatcgtg ggcgtcttcg aattcttgag
     1381 cttgatcggg accaggacgt aattgctggc gttctccgcc gtcgaaggat cgagtggctg
     1441 agagaacttg agggtaatcg ccaagccgtt cttggtgctg acgctggccc gcgcgatggt
     1501 cgccgcgggg ccgacgtcga acacgttgac ggtaacgctt gtcgtctggc tgagctgtgg
     1561 tgagccacta tcggaaacaa ccacaccgat ggaaaaggtc ccgatacgct gccgcgagga
     1621 gagtgtccag gtgagaaggc cggagaccgg gtcgacaatc acacccgaag gtgcacccgg
     1681 gacgaggctg taggcgagca tctgggccgg cgtatcagga tctgacgtgt agcgtgcgag
     1741 attgagctga agcttctgct tgagcacggc ggtctgagtg ggaatggcgg cgatcaccgg
     1801 cggatgattc acgggcagca cattgactgt gaatgtggtc gagtcgctct tgggcacgta
     1861 gccgttgtcg gtcgcgatga tcgtgatcga atacgtccct gagcccgaat aggcgttggg
     1921 cgtccagatg aaacggtgtg actgcgagtc gatcgccgcc ccagccggtg tgcccgcgcc
     1981 cagggtatag ttgatcgatt gggccggcac gtccgaatcg gtagcggtca ggtcgatcgt
     2041 caacgggttc ccttcatcaa cgttctgcgt tggaatcgtc gagagcacgg gcgggtgatt
     2101 gaatggcacg acattgactg tgaaggtctt cgatccgctc agcggcggcg atccgttgtc
     2161 ggtgaccacc acgccgatcg tgtaagtgtt gatgggctgg ttcgattgcg gagtccacgt
     2221 gaacagacct gtggcgtcga tactcgctcc cgagggtgcg cccggagcca aactatagga
     2281 aagcgcctgc gctggaatgt cggggtcgga gacgaagtct cgtaccttga cctggagcgg
     2341 ctgaccttgc tcgacgacct gcgtgggaat gttctgaaga tttggaggat ggtttacggc
     2401 taggacattg gccgtgaagg cgatcgagtc gctcagagga ggtgagccgt tgtcggttgc
     2461 gacaacagtg attgaatagg tgcctgtgct cgagtaggga ttgggcgtcc aggtgaaggt
     2521 ccccgattgt gcaccgatcg acgctccggc gggtgcgcca gggcccaagc tgtaggtgat
     2581 cgtctggctg gggtcagggt cggtcgctga ggcctggaca gtgagcttgg tcccttcatc
     2641 gaccgtctgg tccgcgattg accccagcac aggcgggtga tttacgactt gattcgtgat
     2701 cgccttgatc tgatagatca ccccctggcc agctccactt cccatacttc cgccggatgc
     2761 gatgtcggcg aggtacagcg agtccggggt ggacaagatg tcgtcgatgt ggccgatgcc
     2821 agactcgttg ttcgagacga agtcgaagta atgaccggtg ctcgggttgg cgaagacgag
     2881 cggattttca tcgttggccg tcccgcccgc gtcgaaaact ccgtgaaaac cgatgaaggc
     2941 gccgtgattg agtccagccg gaaagccgga cggagacagt gcgaaccctg aggagccctc
     3001 gctctctgag ccttccgcag ttagaacggg gtcgggtaga ggctggaagg cgaccagcgg
     3061 ctggacgccg acgccagaat tgaccaccgt caccggctcg ccgggcttgt cgatggtctt
     3121 gacgtagctg taagtgagct gtccgttgat cacttcgggg aatccgaaga aaggaacggt
     3181 gacgccgatc tgcgccttgg ggattcgatt cagctcgtcg gcgctccatg cctcgttgcc
     3241 gccattgttg ccgtcgattc cattatcagc aaggtagagg tcgccgctcg tggggtcgat
     3301 cgccaggctc gctgcattgc gcaggcccga cgaaattcgt gtcaggttcg acacgacagg
     3361 attgtggttg tcgtcatgaa gcgtgaccat gtagagcgag tcgccaacga gcgtgccgct
     3421 gatcaagcca ctcgccgaaa cggtcccggt cgacggctcg tatgtcgggt tccccttgtt
     3481 atcgaggacc acgctgccgt tgccgccgat gaccacgccg ttgtactgcg cgccgatgtt
     3541 gaagatcaca tcgaaattgc ccgattgacc tggcgtcggg cggacgacgg acgcaaagct
     3601 ggtgtgctcc cagtttgtcg ggaaggaaaa gttgatgctc ccgaccagcg taagtgaagc
     3661 gcccacggtt gcgccggtgc gaaggacgga aattcgctcg ttgccggtcg cgctgctggt
     3721 cgcgaggatg aactctccgg c
//
LOCUS       DATAQL010001421         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_451247, whole genome shotgun sequence.
ACCESSION   DATAQL010001421 DATAQL010000000
VERSION     DATAQL010001421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451247"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VHS97_24570"
     CDS             <1..274
                     /locus_tag="VHS97_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451450.1"
                     /translation="DPMMPVAWVKTFTGESGKPARIFTTTMGAATDIESPGVRRMLVN
                     AVYWTLGLEDKISSESNVSLVGEYKPRPFGFDGAAKGVKPEVHAAH"
     gene            complement(307..>2073)
                     /locus_tag="VHS97_24575"
     CDS             complement(307..>2073)
                     /locus_tag="VHS97_24575"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX3451451.1"
                     /translation="SANAPAASEDEPHRSPIALALSADGALLLSANQTAGTVSLVDTK
                     GARVLHELKTGDKPSGVAISKDGRRGVVTHWYGYDLCVLSINDGKLAIEGRLEVGPEP
                     RGVAMAADGSTAYVAIGVSNEVARVDLSTRTVTGRLPAGREPRGIAISPDGTRLLVSN
                     ARSQNVSLIDVKGWKVVCTIPIDGDNLRQVTVSADGKTGYIANMRNRGFATTRNNIDL
                     GWVLGQRLTRVPLDGSVSYDTLSLDTQGKAASDAHGVAVSQDERFVAVSCGGTHEVMI
                     FRTDKKRLPWRSNGSRDLIAPELLKKDGRYRRVELGGRPTELAFAPVGSILYVANYLD
                     DSIQVVDAESAELIKTISLGKSKTLSLARRGEILFHDADRSHNAWYSCNTCHSDGHTS
                     GLDFDTLNDGRQDLSSAHLRSRKKAPTLRHVTKTQPWTWHGWQTNLDDAMVESFTKSM
                     QGPKPKADEVTALVAYLDTLEFPKNPFRQPDGSLTPAAQRGRDVFRSAKAACNTCHGG
                     AELTDGKIHEVGLEERDDAYRGYNPPSLRGVYDKDPYLHDGRSKTLHQALSGPHSADN
                     VTGLGELTEQELDDLIAYLKSL"
BASE COUNT          359 a          620 c          685 g          409 t
ORIGIN      
        1 cgatccgatg atgccggtcg catgggtgaa gactttcacc ggtgagtccg gaaagcccgc
       61 ccgaatcttc acgaccacga tgggggccgc gaccgacatt gagagccccg gcgtccggcg
      121 gatgctggtc aatgccgtct actggaccct cgggctggag gacaagatct cgtccgagag
      181 caacgtcagt ttggtcggcg agtacaagcc tcgtcccttt ggcttcgacg gcgccgccaa
      241 gggcgttaag cccgaagttc acgcggcgca ttgagcaagt gccccgatgg cgagagatcg
      301 ccgaattcac aggctcttga gatacgcgat caggtcgtcg agctcttgct cggttagctc
      361 acccagtcct gtcacattgt cggcactgtg tggtccgctc aacgcctgat gcagcgtttt
      421 ggagcggccg tcgtggagat aagggtcttt gtcgtaaacg ccgcggagcg acggcggatt
      481 gtagccgcgg taggcgtcat cgcgctcctc gaggccgact tcgtgaatct tgccgtcggt
      541 aagctcggcg cctccgtggc acgtattgca ggccgccttg gcagagcgaa atacatcgcg
      601 cccgcgctgg gcggcgggag taaggctgcc gtcgggttgg cggaatgggt ttttgggaaa
      661 ttcgagagtg tccaggtatg caaccagggc tgtgacctcg tccgctttgg gcttgggacc
      721 ctgcatgctt ttggtgaacg actcgaccat cgcgtcgtcg agattggtct gccagccgtg
      781 ccaggtccag ggctgggtct tggtgacgtg gcggagagtg ggggccttct tgcgactgcg
      841 gaggtgcgcg gagctcaggt cctgacggcc atcgttgaga gtatcaaagt ccaggccgct
      901 ggtgtggccg tcgctgtggc aggtgttgca gctgtaccac gcgttatgcg agcggtcggc
      961 atcatggaac aagatctcac cacgccgcgc cagcgagagc gttttggatt tccccagcga
     1021 gattgttttg atcagttcgg ctgactcggc gtcgacgacc tgaatcgaat cgtcgagata
     1081 gttcgcgaca tagaggatcg agccgacggg agcgaaggcg agctcggtgg ggcgtccgcc
     1141 cagctcgacg cggcggtacc ggccgtcctt tttgagcagc tcgggcgcga tcaggtcgcg
     1201 ggaaccgttg gaccgccagg ggagccgttt tttgtcggtg cggaagatca tgacctcgtg
     1261 cgtgcccccg caactgaccg caacgaatct ctcatcttga ctgaccgcga caccatgcgc
     1321 atcgctggcg gccttgccct gtgtgtcgag cgacagcgtg tcatacgaga ccgagccatc
     1381 aagcggcact cgcgtcaagc gctggcccaa aacccagccc agatcgatgt tgttgcgagt
     1441 cgtcgcaaac ccgcgattgc gcatgttcgc gatgtaaccg gtcttgccgt cggcgctgac
     1501 ggtgacttgg cggaggttgt caccatcaat cggaatcgta cagacgacct tccatccctt
     1561 gacgtcgatg agcgaaacgt tttgcgaccg tgcattactc acgagcagcc gcgtcccgtc
     1621 gggcgagatg gcaattccgc gcggctcacg gccggccggc agccggccgg tcaccgtacg
     1681 tgtgctcaaa tcgacgcggg cgacttcgtt gctgacgccg atggcgacgt aggcggtcga
     1741 gccgtcggct gccatggcca cgccgcgagg ttcgggtccg acctccaagc ggccttcgat
     1801 cgcaagcttg ccatcgttta tcgaaagaac gcacaggtcg taaccgtacc aatgggtgac
     1861 cacgccgcga cggccatcct tcgagatggc cacgccagaa ggcttgtcgc cggttttgag
     1921 ctcatgcagc acccgcgccc ccttcgtatc caccagcgaa accgtcccgg cagtttggtt
     1981 ggcggacaag agtagtgcgc cgtcggccga gagcgccagt gcgatcggag agcggtgcgg
     2041 ctcgtcctcc gacgcggccg gcgcgtttgc gct
//
LOCUS       DATAQL010001422         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4114960, whole genome shotgun sequence.
ACCESSION   DATAQL010001422 DATAQL010000000
VERSION     DATAQL010001422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4114960"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..1317)
                     /locus_tag="VHS97_24580"
     CDS             complement(319..1317)
                     /locus_tag="VHS97_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3451452.1"
                     /translation="MGFALAEMRRTHALNDGGDELSDEYLLDQFLNGVETESQEAFGT
                     LVERHGPMVLGICRHVLHAHHDAEDAFQATFLVLARKGGSIRNGRVLAGWLHEVAYRI
                     AIKARASAARRRTLERDGVAMAPAAIEPDNQNEVAAWKELRPVLHAEIELLPDRYRIP
                     VILSYLEGKTNEEVAKLLRWPVGTVKGRLSRARDLLRRRLVRRGLALSATFLMTAISH
                     GTVFAEVVPADLVRRTVRLAGKHGARVAQPVPSDSEPPDALEPNPPQSAGATARLKNG
                     LMLVNRQMVARIILTIAAFLITIGIWMAVRGGSGWSSEVRSAMSMVIPAHDGAGCH"
     gene            complement(1602..>2565)
                     /locus_tag="VHS97_24585"
     CDS             complement(1602..>2565)
                     /locus_tag="VHS97_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX3451453.1"
                     /translation="IEQPEKRIELEAAELRCRSLAMEISSWLQHEVKDSVYWVEVVNK
                     GRSRIKLASAPLDVGPSLRRLMFDRVPTCVLTSATLCVGSPPKFGFIKSRLGLTSCEE
                     LALGSPFDYPSQVTVHLPSNLPDPSDQPDLFERRAIRAIAHYLEQTEGKAFVLFTSHK
                     MLEAAARALTPWLAQRNIALFAQSDGMPRSKMVDAFKADINSVIFGADTFWQGVDVPG
                     ESLSNVIITRLPFSVPSHPLLEARLEDIRCRGGSPFVEYQIPEAVIKLKQGFGRLIRT
                     RTDRGIVVILDPRVLNKPYGRTFLNSLPQCPRIVEAPSFGDASA"
BASE COUNT          539 a          781 c          758 g          487 t
ORIGIN      
        1 caacgacgcg ttcaagaagc cgttcgacgg agcgctcctg gccgagcgtg tccgccgctt
       61 gatctcgcag aagaaggccg acgaaccctg aggcacgacg ccaacgggaa cccgacttct
      121 acctcggcag ccaaccgagg cgaacgagcc caacggtctg ctctcacccg atcaaagccg
      181 gtgagggggg ccgtcgggct cgtttgttat tttgacgcag ccgtacgacg ccgcgtgggt
      241 gtgatcgcag cgccactaga tcaatgccgg acgctcaggc gggctcgcag tctcacctta
      301 agaccgagcg agcttgactt aatggcagcc tgcaccgtcg tgtgccggaa tgaccatgga
      361 catggcggac cggacctcag aagaccatcc ggagcccccc cgcacggcca tccagattcc
      421 gatcgtgatc aaaaacgccg cgatcgtcaa aataattcgg gccaccattt gacggttgac
      481 cagcataagt ccgttcttca accgcgccgt cgctccagcg gactggggcg gattcggctc
      541 gagcgcgtct ggcggttcag aatcactcgg aacgggctga gctactcgag cgccgtgctt
      601 gccggcaaga cgcacggtcc gcctgactag atcggccgga acaacctcag caaacaccgt
      661 tccatgcgag atggccgtca tcaggaatgt cgccgacagg gccaggcctc ggcgtaccag
      721 tcgtctccgg agcaggtcgc gagcgcgcga taggcgtcct ttcaccgtgc ccaccggcca
      781 ccgcaagagt ttggcgacct cttcgttcgt cttaccctcc aagtagctca aaatcactgg
      841 gattcggtac ctatcgggta acaattcaat ttcggcgtgc agaacaggtc gcaattcctt
      901 ccacgccgcg acttcgtttt gattgtccgg ctcgatcgct gcaggcgcca tcgccacccc
      961 atctctttca agagtgcgcc gccgggcggc actggctcgc gccttgatcg cgatgcgata
     1021 ggcgacttcg tgcagccagc cggccagaac gcggccattt cggatcgagc cacccttccg
     1081 tgctagcaca agaaacgttg cctgaaacgc atcttcggcg tcgtgatggg catgaagcac
     1141 gtggcggcaa atgcccaaca ccatcggacc atggcgctcg accaaggtcc cgaaggcttc
     1201 ttgagactcc gtttctacgc cgttcaagaa ctggtcgagc aggtactcgt ccgaaagctc
     1261 gtcgcccccg tcgttcaatg cgtgggtgcg acgcatttct gctaacgcaa agcccattct
     1321 cgatgctccc tattcctctg gcgcaaacct cagcacgaat cttggctgcc cgacactcgc
     1381 aacgctttcg gcgaaaacct tttccgcaag tactttatgt cgaacctccg ttggaggttg
     1441 tccatcatcg cgtagggact gtgagccgga aatatcattt cgcgcaatat ttagagggga
     1501 atcttgagcc ggcggttaca gaatcacgga aaaatagcaa acgacctcat gaaagagata
     1561 cgacctcatc aagatggacg cgctgcggac caggggcaag ctcacgccga ggcatcgccg
     1621 aacgagggcg cctcgacgat acgcgggcat tggggcagag agttgagaaa cgtgcggcca
     1681 taaggtttgt tcagcacgcg ggggtcgagg atcacgacga tgccgcgatc ggtccgcgtc
     1741 cggatcagcc tgccgaagcc ctgtttgagc tttatgaccg cttcgggtat ctggtattcg
     1801 acgaaaggac tgccgccgcg gcagcggata tcttccagcc gcgcctccag gagtggatgg
     1861 ctcgggacgg aaaaggggag gcgcgtgatg atcacgttgg agagggactc accgggaacg
     1921 tctactcctt gccagaatgt gtcggcgccg aagatcacgc tgttgatatc ggccttgaac
     1981 gcatcgacca tcttcgaccg tggcatgccg tcgctttgag cgaacaaagc gatgttgcgc
     2041 tgcgccagcc agggtgtgag tgcccgcgcg gcggcctcga gcatcttgtg cgaagtgaac
     2101 aagacgaatg ccttgccctc tgtctgttcc aggtaatggg caatcgcccg gatggccctc
     2161 cgctcgaaca agtccggctg atcggacggg tcaggcaagt tgctgggcag atgcactgtc
     2221 acctggctgg gatagtcaaa cgggctgccg agtgcgagtt cctcgcacga agtcaaccca
     2281 agccgcgatt tgatgaatcc aaatttcgga ggagagccga cgcaaagtgt cgccgaggtc
     2341 aggacacagg tcggaacccg gtcgaacatc aaacgacgta agctggggcc cacgtccaga
     2401 ggggccgagg cgagtttgat ccgagaacgg cccttattga ccacctcgac ccagtaaacg
     2461 ctgtccttaa cctcgtgctg gagccagctc gagatttcca tcgccaaaga gcggcagcgc
     2521 agctcggccg cttcgagctc gattcgcttt tcgggctgtt cgatt
//
LOCUS       DATAQL010001423         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2894416, whole genome shotgun sequence.
ACCESSION   DATAQL010001423 DATAQL010000000
VERSION     DATAQL010001423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2894416"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VHS97_24590"
     CDS             complement(<1..851)
                     /locus_tag="VHS97_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3451454.1"
                     /translation="MCRRFTVLLLVSCVFSGFATDVARADDKANYDHKVDVVYGRKFG
                     MALTMDVFTPKSNANGAAIVWVVSGGWFSAHEAINQGFVGELLKRGYTVFAVVHGSQP
                     RFTIPEIVKDMNRAVRFIRFHAKDYQIDPDKIGITGASAGGHLSLMQGTAGDNGDKTA
                     KDPVDQTSSRVQAVGCFFPPTDFLNYGKPGEIALGCGVLKGFKPPFDFHEQDPTTKTF
                     VRITDLGKILEIGRQISPVYHASSDDPPTLIIHGDADLLVPIQQAELVVEKLKSSGVE
                     TKLIVKKG"
     gene            921..1991
                     /locus_tag="VHS97_24595"
     CDS             921..1991
                     /locus_tag="VHS97_24595"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3451455.1"
                     /translation="MMTPKIQDEKSLDSFRINRRAALRSTITAAGVALVAPESQSAGA
                     NHPEVANAPDPRKSASKSYRMKKSINLWAFPYPQRMSLNECLQLAKEAGFEGIELNYD
                     LDNDLSPKASTNQYRAIRKAAEEIGIAISGLCSFLYWPYSLTDNDPARRKRGLELAGL
                     IIEAAHELGTENVLTIAGSTYIPWIPEREPVAIDVCDRRARESIGQLVPLAEKHGVFL
                     NIENIGFNGYLTTPGELNAFVDSFGSNHIKIHFDTGNVMLFQFPEHWIPLLGRRINNV
                     HFKEFSKKGTDHTLESFRTLLDGTTDWPAVMDALERAGYEGYVTFEYFHPFLHYPEAL
                     IEQTSDALDRILGRPVARRRLS"
BASE COUNT          459 a          661 c          613 g          422 t
ORIGIN      
        1 cccttcttga cgatcagttt ggtctcgacg cccgacgact tgagtttctc gacaacgagc
       61 tcggcttgct ggatcggcac caataaatcg gcgtcgccgt gaatgatcag ggtgggagga
      121 tcgtcggacg acgcgtgata gaccgggctg atctggcgac cgatctcgag gatcttgccc
      181 aggtcagtga tccgaacgaa cgtcttggta gtgggatcct gttcgtgaaa gtcgaacgga
      241 ggcttgaacc ctttcaagac gccgcagccc agggcgatct cgccgggctt gccgtagttg
      301 agaaaatccg tcggcgggaa aaagcagccg acggcctgaa cccggctcga cgtctggtcg
      361 accgggtcct tggcagtttt gtcgccgtta tccccggccg tcccctgcat cagagatagg
      421 tgtccgccgg ccgaggcgcc cgtgatgccg atcttgtccg ggtcgatttg ataatccttg
      481 gcgtgaaatc gaatgaatcg gacggcccgg ttcatgtcct tgacgatctc gggaatcgta
      541 aacctgggct ggctgccgtg gacaacggca aagaccgtgt agccgcgctt gagcaactcg
      601 ccaacaaatc cctggttgat cgcttcgtga gccgaaaacc agccgccgct gacaacccag
      661 acgatggccg caccgttggc gtttgatttc ggcgtgaaga cgtccatcgt gagcgccatg
      721 ccaaacttgc gaccgtagac cacgtcgacc ttgtgatcgt aattggcctt gtcgtcggcg
      781 cgggcaacat cggtggcgaa tcccgaaaag acgcaggaca cgagcagaag gacagtgaat
      841 cttcgacaca tggcgtcgac tccggtagaa cgatggagga gtggtacatt tcgatcaacc
      901 gttcggcaaa ggaagttccc atgatgacgc ccaaaattca ggacgagaag agccttgatt
      961 cctttcgaat caatcgccgt gctgccctcc gatcaacgat caccgcggcc ggcgtcgcgc
     1021 tggtcgcgcc ggaaagtcaa tccgccgggg caaatcaccc cgaggtcgcc aatgcacccg
     1081 atccgcgcaa gtccgcgtcc aagtcgtatc gcatgaagaa atcgatcaac ctttgggcgt
     1141 tcccctaccc ccagcgaatg tcgctcaacg aatgcctgca actcgctaaa gaagccggtt
     1201 ttgaaggcat cgaactcaat tatgatctcg acaacgatct ctcacccaaa gcgtccacca
     1261 accagtaccg cgcgatacgc aaggctgccg aagagatcgg catcgcgatc agcggcctct
     1321 gctcgttctt gtattggccc tattcactca ccgacaacga tccagcccgc cgcaagcgag
     1381 gcctggagct ggcagggctc atcatcgagg ccgcccacga gctcgggacc gaaaacgtac
     1441 tgacgatcgc gggctcgacg tacatcccgt ggattcccga acgagagcca gtcgcgatcg
     1501 acgtatgcga ccgccgcgcc cgcgagtcga tcggccagct cgtcccgctc gccgaaaagc
     1561 acggcgtctt cctcaacatc gagaacatcg gtttcaacgg ctatctcacc acccctggcg
     1621 agttgaacgc cttcgttgac tcattcggta gcaaccacat caaaattcat tttgacaccg
     1681 gcaatgtcat gctctttcaa ttccccgagc actggattcc gctgttgggc cgccggatca
     1741 acaacgtcca tttcaaggaa ttctccaaga aagggacgga tcatactctg gagtcgtttc
     1801 ggaccctgct ggatggaacc accgactggc cggcggtgat ggacgcactc gaacgagccg
     1861 gctacgaagg ttacgtcacg ttcgagtact tccacccatt tctgcattat cccgaggccc
     1921 tgatcgagca gacctcggac gctctggatc gtattctggg ccggccggtc gcgcggcgtc
     1981 gtttgtcgtg atcgaagcgg accggaccga atcgccgtct tatgccgacg tccaggtcgc
     2041 aatctggcca gagcgatacg cgcggttacc gagatgggcg gccgaggcgg cactgacgcc
     2101 ggcttcggcc ggagcattcg ggcgcgcccg catacgaatg cattcgaccc agttt
//
LOCUS       DATAQL010001424         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4269124, whole genome shotgun sequence.
ACCESSION   DATAQL010001424 DATAQL010000000
VERSION     DATAQL010001424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269124"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1530
                     /locus_tag="VHS97_24600"
     CDS             247..1530
                     /locus_tag="VHS97_24600"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX3451456.1"
                     /translation="MADLARCTDLVLARLLHLTNNPSQVLAAMSRHRVGLWLIGAFGG
                     VGTTITLGLAAMVRGLADRTGLVTELPILKRLPLPEPEDFVIGGHDIRRSSFAESAEE
                     FRRNSGVFEVEWLSACRDELAAATARVHPGTHFGAGRAIANLGHWGNAKPPRTAGQAV
                     DRIAADMAAFVEAESVDHMVVLNVASTEPPFSLGAIHQNWNALNSALTDGGPDLLPAS
                     SLYAVAALRSGYTYINFTPSLGASLPALDELAQSTGSLYAGKDGKTGETLVKTVLAPM
                     FAHRNLKVLSWVGHNIFGNRDGLVLDDPSNKSSKVETKDRVVTQILGYKPSSIVTIEY
                     LPDMGDWKTAWDHIHFQGFLGTKMALQFTWQGCDSLLAAPLAIDLARLADLEKVRGGR
                     GLLTHLACFFKSPEQCDENDFFKQFTMLLEHLPAD"
     gene            1618..2463
                     /locus_tag="VHS97_24605"
     CDS             1618..2463
                     /locus_tag="VHS97_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3451457.1"
                     /translation="MRLAFSTNAYLKHPFDDAASRIASFGYEGLELLADVPHAWPAGL
                     LAVQKRGILEAMERSRLAFSNINAFMMNAVNDRRQPYWHPSFIEPERHYRQVRIDHTK
                     RALDLCAELGAPHITTEPGGPLAPGQPRQEAIDLFVEVLKPLAEHAHERGVLLLIEPE
                     PDLLLETTDQYLEVAERLNAPSIGLNFDVGHAFCVREDLPRAISKLAPHIRHYHFEDI
                     AATRVHHHMVPGTGAIDFAAVMAAIRATGYDGWLTVELYPFLDDPDTAARTALEVLRP
                     LVRRV"
     gene            2521..3417
                     /locus_tag="VHS97_24610"
     CDS             2521..3417
                     /locus_tag="VHS97_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX3451458.1"
                     /translation="MPYLQLIRLPNVVTAAADSLAGWLLATGSTSDPGRWLPLIAASM
                     VLYASGIALNDVFDIEIDRSERPGRPLPSGRVSYKTAAWFGGIGLVIGPALALVSGRV
                     SSIIVAAILALFILAYNAGLKHTWLGPVFMGSCRGLNLLLGMSHAQALGGPIAWSAAV
                     AYGLYVLGITVVSRSETSGGIRGGLVAGLGLQDLALLGLAGVTFAHNHFPQPDPEQPL
                     IPLEGLLVLALVALAVNSTAARAINEPVPQLIQKTVKTGILSLVWLHVGVVAGVRGLE
                     LAAYVAIVWVPAYLLGRWLYST"
BASE COUNT          679 a         1199 c         1082 g          734 t
ORIGIN      
        1 ggacgctttc ataaaaggaa cccgaccctt tttggtctgc gccgcgacga atcgcggcag
       61 gcaaaggcat ggccgcaacg aaaaccgacg aggggtcatg ccctgacccc aaacgtgcgc
      121 cgtgccagca gagaggtcaa cgcatcgtcg ctcacttcgc tctcatttgc gggaaggttg
      181 aatgccccgc aggatggggt cataataaga attcgtatgc gagcgtttca ggtcaggtgg
      241 ccgcatgtgg ccgacttggc ccgatgcacc gacctcgtgc tagcccgcct tctgcacctt
      301 accaataatc cgtcacaggt tctcgccgcc atgtcacgtc atcgcgttgg cctctggtta
      361 attggggcat ttggaggggt cggcacgaca atcacgctcg gtctggcggc gatggtcagg
      421 gggttggccg accgaaccgg tctcgtcacc gagttgccca tcttgaaacg gctgcccctg
      481 cccgagcccg aggattttgt gattggcggg catgacatcc gccggagttc attcgctgaa
      541 tcggccgagg agtttcggcg aaactcaggt gtgttcgaag tcgagtggtt gagcgcgtgc
      601 cgcgacgagc tcgcggcggc aaccgcgcgc gtacatccag gcacgcactt cggcgcgggt
      661 cgcgcgatcg ccaatctcgg tcactggggc aacgcgaagc cgccgcgtac agccggccaa
      721 gccgtcgacc gcattgcggc ggatatggcc gcgtttgtcg aggcggaatc agtcgaccac
      781 atggtcgtgc tgaacgtcgc cagcaccgaa cctcccttta gcttgggcgc gatccaccag
      841 aactggaacg cgttaaactc cgcgctcacc gacgggggcc ccgacctgct tcccgccagc
      901 tcgctttacg cggtggccgc cttacggtcc ggctatacct atatcaactt cactccgagc
      961 ctgggcgcct cgctgcccgc ccttgatgag ctggcccaat cgacgggctc gctttatgcc
     1021 ggtaaggacg gcaagacggg cgagacgttg gtgaaaactg tcctcgctcc catgtttgcg
     1081 caccgcaatc tcaaggtctt gagctgggtg ggccacaaca tcttcggcaa tcgcgacggc
     1141 ttggtcctcg acgatccgtc gaacaagtct tcgaaagtcg aaaccaaaga ccgggtcgtc
     1201 acccagatcc tgggctacaa gccgtcgtcc atcgtcacaa tcgagtatct gccggacatg
     1261 ggcgactgga agaccgcctg ggaccatatt catttccagg gattcctcgg caccaagatg
     1321 gcacttcagt tcacgtggca aggttgtgac agcctactgg cagctccgct ggccatcgac
     1381 ctggcacggc tggccgacct cgaaaaagtg cgcggcggcc gcgggctttt gacccacctc
     1441 gcttgctttt tcaagagccc cgagcagtgc gatgagaacg acttcttcaa gcaattcacc
     1501 atgctgctcg agcatttgcc ggccgactga gccccgcgcg acggcaccgc ggcgatccat
     1561 caacggtgat cttctaattt gtgtccctcg atccttgata agaaagcacc ccgcgccatg
     1621 cgcctcgcct tcagcaccaa cgcctacttg aaacatcctt tcgacgacgc agcttcccgc
     1681 attgcttcgt tcggttacga ggggttggaa ctgctcgccg acgtacccca cgcctggcct
     1741 gccggtctgc tggccgtgca gaagcgggga atactagagg ccatggagcg atcccgtctg
     1801 gcgttttcga acatcaatgc tttcatgatg aacgcggtca acgaccggcg ccagccttac
     1861 tggcatccgt cgttcattga gcccgaacgc cactaccgcc aggtccggat cgaccacacc
     1921 aaaagggcgc tcgacctgtg cgccgagctt ggcgcgcccc atatcaccac cgagcccggc
     1981 ggtcccctgg caccgggaca gccgcggcaa gaggccatcg acctcttcgt cgaggtcttg
     2041 aagcccctgg ccgagcatgc ccatgagcgg ggagtcttgc tcctgatcga gcccgagccc
     2101 gacttgctgc tggaaacgac cgatcaatac ctcgaagtcg ccgagcgatt gaatgcgcca
     2161 tcgatcggcc tcaacttcga cgtaggccat gcgttttgcg ttcgcgaaga cctgcctcgc
     2221 gccatctcca agctggcgcc tcacattcgc cactaccact tcgaggacat cgccgcgacc
     2281 agggtgcatc accacatggt gccgggtaca ggcgccatcg atttcgccgc ggtgatggcg
     2341 gccatccgcg ccacgggcta cgacggctgg ctgacggtgg agctctaccc gttccttgac
     2401 gacccggaca ccgccgcgcg tacggctctc gaggtattgc ggcccttggt ccgaagggtg
     2461 tagatcgtgg cggaaaactt acggcagtca gaatcgtccg cgacgttttg gcagacgata
     2521 atgccctacc tccaactgat ccggctgccc aacgtcgtga cggccgcggc agatagcctg
     2581 gccggctggc tgctcgcgac tgggtcgaca tccgacccag ggcgctggct gccgctaatc
     2641 gctgcatcga tggtgctcta cgcctccggc atcgccctta atgacgtgtt cgacattgag
     2701 atcgatcgat ccgaacggcc gggccgccct ctgccgtccg gacgcgtgtc ctacaagacc
     2761 gctgcctggt ttggtggcat cggactcgtg atcggcccgg ctctggccct cgtgagcggg
     2821 agggtgagca gcatcatcgt cgccgcgatt ctcgcgctct tcatcctggc ttacaacgcc
     2881 ggtctcaagc acacctggct cgggccggta tttatgggct cttgccgcgg actgaacttg
     2941 ttgctgggca tgtcgcacgc ccaagcactc ggtggtccga tcgcctggtc cgcagccgtc
     3001 gcatatggtc tgtatgtcct gggaatcacc gtggtgagcc ggtccgagac atcgggagga
     3061 atccgcggcg gcctggtggc gggcctgggg ctccaggacc tcgctctgct cggcctggcc
     3121 ggcgtaacct ttgcccataa ccacttccca cagcccgatc ctgaacaacc gctcataccg
     3181 ttggaaggac tccttgttct cgccttggtc gcgctggcag tcaattccac cgctgcgcga
     3241 gcgataaacg agcccgtccc tcagctcatc cagaaaacgg tcaaaactgg catcctctcc
     3301 ttggtctggt tgcacgtcgg agtcgtcgcc ggtgtacggg gattggaact ggcggcttac
     3361 gtggcgatcg tgtgggtacc cgcctacttg ctagggcgat ggctctattc cacctgagat
     3421 ggcttcgaac catgccatgc catgcgactc gtgcgtcggc tggccttcgt gacccatccg
     3481 tgaaataata ggacggattg tccgatctca agtcgtcccc tgaggcctga accgatcatg
     3541 acgactgcaa cccaacaaac cacgcaatcg gcgatcgagt accccgacgt tgacggcgag
     3601 cccatgtcgg acaatacgtt gcaagtcaag tggatcgtcg tcatcaagga acgccgcgag
     3661 gcccttttcg gccacaatcc ccaggttttt gtag
//
LOCUS       DATAQL010001425         5114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3811601, whole genome shotgun sequence.
ACCESSION   DATAQL010001425 DATAQL010000000
VERSION     DATAQL010001425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3811601"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VHS97_24615"
     CDS             <1..244
                     /locus_tag="VHS97_24615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3451459.1"
                     /translation="ELTKFHTPPAVRAEPKNAPANALGIRRIMFAVDDIDDIVARQRS
                     HGAELVGEIAQYEDIYRLCFLRGPDGIIIGLAEQLS"
     gene            complement(373..1659)
                     /locus_tag="VHS97_24620"
     CDS             complement(373..1659)
                     /locus_tag="VHS97_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3451460.1"
                     /translation="MSNWDVFHGDRLELERGLSAAAILDALARGDLRDDDLVRPAGTT
                     VAWARLAELPELLESARAGSDQPASPVAPIQTAPLPPNSIPPSESQGDFEIQVAESGA
                     TPVLSIEPTLRPQDRFALRPDSDVTFPVINDVPADAKFLPGGANNPVLPGAGGWVWAE
                     TDDEADEEEDDNDFDGDLEIVEDLGADDLEILDDESDVGDDVSVAVVKHHEEESRSSR
                     HDQESRSSRVALPVVNARGWDDTGADEELPDEDAFSLSRSGPATVEELDLAPMVDVAF
                     QLVLFFMVAAQTVLYKTLEIPKPSQEQAPSAVAQGRSRTLDDLKEDFVLVEIDSSGAM
                     KIDRQPVAADMNALVERLRTARESTHRKAMLLSADFATPHRNSVLAYDAANEIGLGIV
                     IARPANPQGPAPSLGPAQPAAKSPAPPAAAAGSEPN"
     gene            complement(1702..2136)
                     /locus_tag="VHS97_24625"
     CDS             complement(1702..2136)
                     /locus_tag="VHS97_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3451461.1"
                     /translation="MLLRRDVDRELNEHIDMTPMVDVVFQLMTFMLFSVQMTGGEKVD
                     VPPARHGVGVEETAATFLTLTKPRVPGGETQLLVGLGEGPVATLEQARQAVADGAKEG
                     RRKVILQADGDVPHGEVVKVAGAVAQVEGITVHVGVQEPQGH"
     gene            complement(2153..2695)
                     /locus_tag="VHS97_24630"
     CDS             complement(2153..2695)
                     /locus_tag="VHS97_24630"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3451462.1"
                     /translation="MLYRSLAKKSIREPGVLIDQVRDLCQARRFDAAINLCQSPPYWH
                     TALAQLIAVALRSRSKGLAKIKQLVVTEFHSEVIAGMENRLGSVSTIVRMGPLLGLVG
                     TVASMIAAFSRIGGSEKADPHALATDISLALWATGAGLLIANPMMLIGNDVHARLRRL
                     RDRTERQLQDVLEILEPNQN"
     gene            3186..4034
                     /locus_tag="VHS97_24635"
     CDS             3186..4034
                     /locus_tag="VHS97_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3451463.1"
                     /translation="MPRTVLVVDDERDTNDILANLVQARGLEPVQVFSGEQALAAVAA
                     TTPDLILLDLMLPDIDGFELCDRLKRARETNLIPIVMVTALQDAHHRATGVRVGANGY
                     LSKPFTPAQLYEIIDTAFAWHDEHQQRGTTGEINFDVRSELTYLSQASDMLADLFAHT
                     PLTDRHLKDLKQAVMEMGGNAIEWGHRKNAELVLRITYRIDPNAVTLIIKDQGPGFNP
                     RNLPHAASDEDPIGHIDIRNELGIREGGFGIMLARGLVDKFWYNKLGNEVTLVKYFEP
                     QKQTES"
     gene            complement(4124..4768)
                     /locus_tag="VHS97_24640"
     CDS             complement(4124..4768)
                     /locus_tag="VHS97_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451464.1"
                     /translation="MLRITTVAAAVGLITLGIQGLAPCARGQAIAFEPVVGFVPTGST
                     LTVTPVVSADRRYVRLSVNPFFNALNGFSTFGIPGAVSGGPSGNAGMNGVIGPVGFGD
                     GSGINPYGFVGNVGEMRAGPIPMNGGLGAGNSLAQNDGIMEAPAAVMNGGPEGAFDGS
                     QAWPLGMTNRSVASGGQRGKSSAKAVSRQRRTARKAATKSKSTSTTEPSIRPAR"
BASE COUNT         1041 a         1587 c         1499 g          987 t
ORIGIN      
        1 tgagctgacg aagttccaca cgccgccggc ggtcagagcc gagccgaaaa acgccccggc
       61 gaacgcgctg ggcatacgcc gcatcatgtt cgccgtcgac gacatcgacg acatcgttgc
      121 ccgccaacgc agccacggtg ctgaactcgt cggcgagatc gcgcagtacg aggacatcta
      181 ccggctctgc ttcctgcgtg gccctgatgg catcatcatc ggactggccg agcagctcag
      241 ctaaaacatt agacgcaggc cacgccgccg acgacaacag acaagacttt tcggatcggg
      301 acgccgatgc gaaaagtctt gtctgttggt gccgacctct gcgatagctc catggccgta
      361 cgccaccgga attcagttcg gctctgaacc tgctgccgcg gcggggggag cgggtgactt
      421 ggcggcgggc tgcgccggac ccaagctcgg ggctgggccc tgcggattcg ccggacgggc
      481 gatgacaatg cccaggccaa tctcattggc cgcgtcgtac gccagcaccg aattacgatg
      541 cggcgtcgca aaatccgccg aaagcaacat cgccttgcga tgggtcgact cgcgcgctgt
      601 tcgcagccgc tcaactaggg cgttcatatc cgccgcaacc ggctggcgat cgattttcat
      661 cgcgccggac gagtcgattt cgaccagaac aaaatcctct ttgagatcgt cgagcgtacg
      721 cgaccgaccc tgcgcgaccg cgctcggcgc ttgctcttgg gagggcttgg gaatctcgag
      781 cgtcttgtaa agcacggtct gggcggccac catgaagaag aggacgagct ggaaggcaac
      841 gtccaccatc ggcgccagat cgagctcttc gacggtggca ggcccgctgc gcgaaagcga
      901 gaaagcgtct tcgtcaggaa gctcctcatc ggccccggtg tcgtcccagc cgcgcgcgtt
      961 gacgacggga agagccacac gactcgaacg actttcttga tcgtggcgac tggagcgact
     1021 ttcttcttcg tggtgcttga caaccgcgac tgaaacatcg tcgccgacat ccgactcatc
     1081 gtcgaggatc tcgaggtcat cggctcccag gtcctcgacg atctcgaggt cgccatcaaa
     1141 atcattgtca tcctcttcct catctgcttc atcgtcagtc tcggcccaaa cccatccgcc
     1201 cgcaccgggt aacacaggat tgttcgcgcc gccgggcagg aacttcgcgt cggccggaac
     1261 atcattgatc accgggaagg tcacgtcaga atcgggtctc agagcgaacc ggtcctgtgg
     1321 gcgcaacgtc ggttcgatcg aaagcacggg agtcgcgccg gactctgcca cctgaatctc
     1381 gaagtcaccc tgagactccg agggtggtat cgaattcggc ggcaaaggcg ccgtttgtat
     1441 gggcgcgacc ggcgacgcgg gttgatctga acctgcgcgg gctgactcga gcaattcggg
     1501 caattccgcc aaccgcgccc aggcgacggt cgttcccgca ggccgaacca ggtcgtcgtc
     1561 gcgcagatcg ccgcgtgcaa gcgcgtccag aatcgcagcc gcactcaggc cgcgctcgag
     1621 ttcgaggcgg tcaccgtgga agacatccca attcgacaat gggctctcca tcgcagggta
     1681 cttcgcgggg accttctacg gctagtggcc ctggggttcc tgaaccccga cgtgcacagt
     1741 gatcccctcg acctgagcga ccgcgccggc aaccttgaca acttccccat gcggaacatc
     1801 gccgtcagcc tggagaatca ccttgcggcg accctccttg gccccatcgg cgaccgcctg
     1861 gcgcgcttgc tcgagggtgg ccactggccc ctcacccagc cctacaagaa gctgcgtctc
     1921 gccccccggt acccttggct tggtcagcgt taaaaaggtc gcggccgttt cctccacacc
     1981 cacgccatga cgagctggcg gtacatccac cttttcacca ccggtcatct gcactgagaa
     2041 cagcatgaag gtcatcaact gaaaaacgac gtcgaccatt ggggtcatgt cgatgtgttc
     2101 gttgagctcc cgatcgacgt cgcggcggag caacatggta gaatcgggac ctctagttct
     2161 ggttgggctc gaggatttca aggacgtcct gaagctgacg ctcggtgcga tcgcgcagcc
     2221 gacgcagccg agcatggacg tcgttaccga taagcatcat ggggtttgcg atcaataaac
     2281 ccgcgccagt cgcccagagc gccagactga tgtcggtcgc cagcgcgtgc ggatctgcct
     2341 tctccgaccc accgatgcgg ctgaacgcgg cgatcatgct ggcgaccgta ccgaccaagc
     2401 ccaagagcgg acccatccgc acgattgtcg agaccgagcc gagacggttt tccattcccg
     2461 cgatcacctc gctgtgaaac tcggtgacga cgagttgctt gatcttcgcc agccccttgg
     2521 atcggctgcg caatgccacc gcgatcaatt gggcgagcgc cgtatgccag taaggggggc
     2581 tttgacatag atttatggcc gcatcgaacc gccgcgcctg gcacagatcc cgaacctggt
     2641 cgatcagaac acccggttcc cgaatcgatt tcttggcgag acttcggtac aacataattc
     2701 cattatagac cccccagacc gcctaaagcg ccaaggaaac atagatgacg taaccaaaag
     2761 catcgatgat ttttgtgaca atcatggccc tgccatctgt gcgaacacga tcaacagttg
     2821 tgccgaggat gtgaaaaccg taaccgtcat tagcaatacg cagggagggt cactcacgga
     2881 cttgccgcct gtaatattat tgtgccgggc ttggtcagtt gccgcaaggg agtgtgacaa
     2941 cggctcgact gtggtcggat cggagtccga gtcttccgtc gcttccgcgc cgacggggcg
     3001 caggacgtct ggagtttcgg attctgcgga tcgatctgcg ggccgcaata gctatcacgg
     3061 tggctctggt gttccaatgc cttgccacgc tgagcttggc gcgagagaat atggtttccc
     3121 agccttggga tcgtgatacg gcacaggaag cggtcgaact tccgcagtct cttcgagttt
     3181 tccccatgcc gcgcaccgtt ctggtggtcg acgatgagcg cgacaccaac gatatcctgg
     3241 ccaatctcgt tcaagctcgt ggtttggaac cggtccaggt tttttcgggt gagcaagcac
     3301 tcgctgcggt cgccgcaacg acaccggacc tgatcctcct cgacctgatg cttcctgaca
     3361 tcgatggctt cgagctttgc gaccgattga agcgtgcgcg agaaacgaat ctcattccga
     3421 tcgtcatggt gacggcactg caggatgcac accatcgggc gacaggcgtg cgggttggag
     3481 cgaatggcta tttaagcaag ccgttcacgc ccgcgcagct gtacgagata attgatacgg
     3541 cgttcgcctg gcatgatgag caccaacagc gaggcacgac cggcgagatc aatttcgatg
     3601 tccgtagtga gctgacctac ctgtcacaag ccagcgacat gctggccgac ctgtttgcac
     3661 acacaccttt gaccgaccgc cacttgaagg atctgaagca ggccgtcatg gaaatgggcg
     3721 gcaacgcgat cgagtggggt caccgcaaga atgcagagct tgtgctacgc atcacctacc
     3781 ggatcgatcc caacgcggtc actctaatca tcaaggacca agggcccggc tttaatccca
     3841 ggaatttgcc tcacgccgcg tctgatgaag acccgatcgg ccacattgac attcgcaacg
     3901 aactcggaat ccgcgagggg ggctttggca tcatgctcgc cagggggctc gtggacaagt
     3961 tctggtacaa caagctcggc aacgaggtca ctctggtcaa atacttcgag ccgcagaaac
     4021 agaccgagag ctgagatcgg ttcgacttat cgttcgccgg gatcgccgtc atcaagactc
     4081 acccacaccg tagtgagctt tgcgtgcagt gtggggactc gaattaacgt gcgggacgaa
     4141 tggacggctc ggtggtgctt gtggacttgg attttgtggc tgccttacgg gcagtgcggc
     4201 gctgcctgga aacggccttg gcggaggatt tgccgcgttg gccgccgctg gcgaccgagc
     4261 gattggtcat tccgagcggc cacgcctggg acccgtcaaa cgcgccctca ggcccaccgt
     4321 tcatcacggc tgccggcgct tccatgatcc cgtcgttctg agcgagagaa ttgcccgcgc
     4381 caagcccacc attcatgggg atcggacctg ccctcatctc tccgacattg ccgacgaatc
     4441 cgtatgggtt gatcccgctg ccatcgccga atccaaccgg cccgataaca ccattcatcc
     4501 cggcgttgcc actggggccg ccactgacag cgcccgggat cccaaacgtg ctgaaaccat
     4561 tcaaggcatt gaaaaatggg ttaacgctca ggcgaacata gcgccggtcg gcggagacga
     4621 cgggagtaac cgtgagagtc gatccggtcg ggacaaagcc tacgaccggc tcgaatgcga
     4681 tcgcttgacc ccgcgcgcaa ggcgcgagac cctggatacc caacgtgatc agtccgacag
     4741 cggccgctac cgttgtgatc cttagcatgt caggcctcgt cttcgtctgt gagactccgc
     4801 tcggtcgtca gatctgccaa aaacctaatt atcggcaatc agcaactcgt attatgccgc
     4861 gattggccac gagcgaccag accgaagtca attattgcgc gatccgcgca tcgcctttgg
     4921 cgacctcctg catcgtagac cgccggcctc ggtgaaccgt ccatctcgaa cggctcaaga
     4981 gttcgttctt cccaggcccg ggatgatcca gcacgagtcg tgtgagtgca taccgatctt
     5041 gggatagtaa gtacgtgcct tgggagcagc cagcaagatc agggtcgtct gcagtcctgc
     5101 gtattggtgg gtgc
//
LOCUS       DATAQL010001426         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2743950, whole genome shotgun sequence.
ACCESSION   DATAQL010001426 DATAQL010000000
VERSION     DATAQL010001426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2743950"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            802..999
                     /gene="csrA"
                     /locus_tag="VHS97_24645"
     CDS             802..999
                     /gene="csrA"
                     /locus_tag="VHS97_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX3451465.1"
                     /translation="MLVLSRKLGEKIVIGDNIVVTVVKIDRNQIRIGIEAPSDVPVYR
                     EEIAPQRLPGATRGDAVLVSR"
     gene            1382..>3709
                     /locus_tag="VHS97_24650"
     CDS             1382..>3709
                     /locus_tag="VHS97_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX3451466.1"
                     /translation="MLATWIETAAKGRLGGPVRAGMWSAAVIGTHPVQDNQRVWLQLA
                     ADDLILGRLPAYWIENKGGNSFWHAPIPPQAVGIRLHYRAIVERVDGVSAESTYQDTI
                     VRPNLPDRTESVDSLLLGAEGLVGNRLMTVRIDARGSTYDVYFPTVGLHSYVRPKEGD
                     LPQSRCHFRGIIGGLAVGRRLDWFTERGAWDSYQQYQGATNLLTTKLTWKQGPIQVLI
                     TDFAAMGDCLPSNAGREKSPGQYIKRFLIKNEGNQARRAVFAVYVQAEVNGGVGDVGL
                     SWHDIDRSLLAINRGHIHANRKLARDSTIEFALAFDGRGDVECEPTGPNEAILFRTIE
                     LPAGGGVTVDMLVSGAFTGWSGDRGTFEHWLRPALNWFRSADLDLVEHTTAREWDDFI
                     EPLPDLHFPKPTFAVHLRRSALAAALHADAERGGIASGFDRGLSAYCWPRDAIWVGGA
                     LERLGHPSIGRGVYQWLNKVRQRHRPFLYWFQKYSLDGVPEWETPAVDQTALIPWGLE
                     RHYRRTGDLDLVASVWPMIDQAATVCCGNSGGHPGLMLLDDLNLITSAASGNQLFGAF
                     LYSNACVVAGLRSAARLATQLGVNNSAERWIAFADRIWNDGILRVIASSRSGSPGMVD
                     PESGRFLQARRLSTLRGFWTDNPDFLIDHSTSLDINMLGVAAPFGLLPASDPRLTKTA
                     ESILRANDALKGDSTVLARATFDLTASNRTGSHSDQHDVSSLATLWMVRYLIQLGRET
                     GQARHWSRALVMLEGILSRLSQLGLMLRSPGRSMES"
BASE COUNT          735 a         1109 c         1104 g          761 t
ORIGIN      
        1 tcaaatcata gagcggctat ggccgatgtc gccggaaaca gagaaacggg aggcagtgtg
       61 cccacctccc cctattcaac cgacggcaaa ggaaggattt atcaattggg aagtaaagta
      121 tcaaatttcc gaggggacag acctagccgt gagcgccact tcaatcggtt tgtaccccgc
      181 aacccagagt caggttcccg tcgacatggg ctaccgcggg ctcgggaatg aacgtttgcg
      241 acgtgattcc caaccccgcc ccgtgcgcgc tatatacctg atgccgaagt ccgcggcggc
      301 atgtgctgga cggtctcgga atgctcgtaa accaagacgt catcgccttc aagctctggt
      361 cgcgcttgtc tgctccaccc tcagtcacga ggcactttct cgcgcccctt tttctgccgc
      421 ttcctcggat gagcacgacg ggaccgagtc tgcgcacgtc agagttattt tcgttcacgc
      481 gcctttccgc agactggtgt tttccttctt ctcacaacct acaaataggc cgacaaatag
      541 gccgagcgca aactattctt ctcgttttta cacaaggaca cggagaagga gcgaggttgt
      601 tagcaccttt tgttcagtaa tcaggcggtc gcgccggtca acaattggaa gcaaaacatt
      661 atcagagtca cctttatgat aacgatcaaa ctcgtattcc aggtgttggc ataaaagatg
      721 cctgtaatag ataatgaatg gtttacaggc gaacgatgac ccaagttttc gtcgtggaaa
      781 gttcaaccgg gagatcacta tatgctggtt ctaagcagga agctgggtga gaagatcgtc
      841 atcggtgaca atattgttgt tacggtggtt aaaatcgatc gcaatcaaat ccgaatcgga
      901 atcgaagccc ccagcgacgt gccggtctat cgcgaggaga ttgctccgca gcgtctccca
      961 ggtgccactc gtggcgacgc cgttctcgtc agcagatagg cagcgcggcc ttcacgctaa
     1021 gccaccatga tcgcggtgtt cgatgtcggg gaactcgagc ctcacagaca gctcgcgaag
     1081 tacgtattgt gttccggggg accccacccg ctaaggtccc cgcccgacgc cgagttcgaa
     1141 cgccataccc acggcggatt gcctgtcgac atttcccagc cggtttgacc tgcccgcggc
     1201 tcgcggtgct attttgtggc cggttcgcgg gccacgcgag tcgggctccc cttggcgcac
     1261 ctaccgcgaa acaccagaat cgatcgttca cctccatgcg aggagaacgg aatcgaaagg
     1321 aagccgagtc ggatctcctt tcgagagcgc gacgtcggta agggtgcgag gcgattgact
     1381 gatgctggcg acctggatcg aaacggcggc caaaggccgt cttggcggcc ccgttcgagc
     1441 gggaatgtgg tcagccgcag tgatcggcac ccatccggtt caggacaacc agcgagtctg
     1501 gctgcagtta gccgccgacg atctgatact cgggcgcttg ccggcctact ggatcgaaaa
     1561 caaagggggc aacagctttt ggcacgcgcc gatccctccg caagcggtgg ggatcaggct
     1621 ccattaccgc gcgatcgttg agcgtgttga cggagtctcc gccgagagca cataccaaga
     1681 tacgatcgtg cgccccaatc ttccggaccg aactgaatcg gtcgattcgc tgctcttggg
     1741 agccgaggga ttggtcggta accgattgat gacggtccgg attgacgcgc gtggttcgac
     1801 ctacgacgtc tactttccca ccgtcggcct tcattcctac gtccgtccca aggaaggaga
     1861 ccttccccag agccgctgcc atttccgggg gatcatcggt ggtctcgcag tcggacgccg
     1921 gctcgactgg ttcacggaac ggggggcgtg ggactcttat cagcagtacc agggagcgac
     1981 aaaccttttg acgacaaagc tgacctggaa gcaggggccg atccaggtct tgatcaccga
     2041 ctttgcggct atgggcgact gcctgccctc aaacgcgggc cgggaaaagt cgcccggcca
     2101 gtacatcaag cggttcttga tcaagaacga aggcaaccag gcccgtcgcg cagtcttcgc
     2161 cgtttacgtg caggccgaag tgaacggcgg agtgggggac gtgggcctga gctggcacga
     2221 catcgatagg tcgctgctgg cgatcaaccg cggccatatc cacgccaacc gcaagctggc
     2281 gagggattcg acgattgagt ttgcactggc gttcgacggc aggggagatg tcgagtgcga
     2341 gccgaccggt cccaacgagg cgatactctt tcgtaccatc gagctccccg ccggcggcgg
     2401 agtgacggtc gacatgcttg tgagcggagc gttcacagga tggagcggcg atcgcggaac
     2461 ttttgagcac tggctccgcc ccgcgctcaa ctggtttcgc tcggccgatc tcgacttggt
     2521 cgagcacacg accgccaggg aatgggacga tttcatcgag cccttacccg acctgcattt
     2581 ccccaaaccg acattcgccg ttcatctgag gcgctcggcc ctggcggcgg cgctccatgc
     2641 cgacgccgag cgggggggca tcgcgtcggg tttcgatcgc ggattaagtg cctattgctg
     2701 gccacgcgat gcgatctggg tcggcggtgc cttggagcgc ctgggccacc cctcgattgg
     2761 acgcggagtt tatcagtggc tgaacaaggt ccgtcagcgc catcgcccgt tcctctactg
     2821 gttccaaaaa tattcattgg acggagtgcc cgagtgggaa actccggccg tcgaccagac
     2881 agcattgatt ccgtggggac tcgaacggca ttatcgccgc accggcgacc tggacctggt
     2941 tgcgtcggtc tggccgatga tcgaccaggc ggcaacggtt tgttgcggga actccggcgg
     3001 ccatcctggc ttgatgctgc tcgatgacct gaacttgatc acctccgcgg cgagcggcaa
     3061 tcagcttttc ggtgcttttc tttattcaaa cgcttgtgtc gtggcgggtc tgcggtcggc
     3121 ggcgcggttg gctacccaac tgggtgtgaa caattcggcc gaacgctgga tcgcgtttgc
     3181 ggatcgaatc tggaacgacg gtattctcag ggtgatcgcg agcagccgtt ccggcagtcc
     3241 cggcatggtt gatcccgagt cgggccgctt cctccaggcg cggcggctct caacattgag
     3301 gggcttttgg accgacaacc ccgacttctt gatcgaccat tcgaccagcc tggacattaa
     3361 tatgctgggc gtggctgccc ctttcggcct tcttcccgcg tccgatcccc gcctgaccaa
     3421 gaccgccgag agtattctcc gcgccaacga cgctctcaag ggcgattcga ccgtgctggc
     3481 acgcgccaca ttcgatctca ccgcgtccaa ccggaccggc tcgcacagcg atcagcacga
     3541 cgtatcgagc ctggcgacgc tctggatggt cagatacttg attcaactcg gccgcgaaac
     3601 aggacaggcc cgtcactggt cacgtgccct cgtcatgctc gaaggcatct tgagccgact
     3661 ctcgcagctt ggactcatgc tgcggtcacc cggccgtagc atggaatcc
//
LOCUS       DATAQL010001427         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_147602, whole genome shotgun sequence.
ACCESSION   DATAQL010001427 DATAQL010000000
VERSION     DATAQL010001427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147602"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VHS97_24655"
     CDS             complement(<1..613)
                     /locus_tag="VHS97_24655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451467.1"
                     /translation="MLHATEIIGAETYDSFGNYVGRVKEMFVEPADQPNRVAKLLLGR
                     GQYRALLARYDQIGSVAPGKIKLTTDESALELYQPNEAWLAVGKDLLDQQIIDTNGRK
                     VVRVNDIDLADQRTNGNTELRITQVDVGLPGAVRRLLQGIVPPMAIRRIQKRLPPRKI
                     LWEFVNLIEPDPLRRVKLRLSSDKLEALHPADLADIMEELSPDE"
     gene            complement(1025..1645)
                     /locus_tag="VHS97_24660"
     CDS             complement(1025..1645)
                     /locus_tag="VHS97_24660"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX3451468.1"
                     /translation="MPQSRKTNSEIEVKLRVTDVHATARAIKSLGAASRGRVFEQNAL
                     YDTPQSDLRRRRWLLRVRMEASAKPGASNGRIRRVLLTSKAPLQQAYGTRKSPRYKER
                     AEREVVADPSRSWPASLAALGFKPSFRYEKYRTSFRLRGLHMDLDETPVGVFLELEGQ
                     PRAIDRVARALGYTPADYIRGTYWDVYAADCRHRGRDPKNMLFPAK"
BASE COUNT          298 a          485 c          552 g          367 t
ORIGIN      
        1 gctcgtctgg cgaaagctct tccatgatgt cggcgaggtc cgcagggtgc aacgcttcca
       61 gtttgtcgct cgaaaggcgc agcttcacgc gacgcagcgg gtccggttcg atcagattca
      121 cgaattccca gaggattttt cgcggaggta gtcttttctg gatgcggcgg atggccatgg
      181 gcggcacgat tccctgcaat agacgccgaa cggcaccggg caggccaaca tccacttgcg
      241 tgatgcggag ctcggtgttg ccattggtcc gctggtcggc aaggtcgata tcgttgacgc
      301 gcacgacttt tcgtccgttg gtgtcaatga tttgctggtc gagtaggtct ttgccgacgg
      361 cgagccaggc ttcgttgggt tggtagagct cgagcgcgga ttcgtcggtg gtcaacttga
      421 tttttccagg agcgaccgaa ccgatctggt cgtagcgggc cagcagcgcg cgatattggc
      481 cgcgacctag gagcagcttg gcgacgcgat tcggttggtc ggcgggctcg acgaacattt
      541 ctttcacgcg gcccacatag ttgccgaacg aatcgtacgt ttcggctccg attatttcgg
      601 ttgcgtgcaa catggtgact cctttacagc atgctcaaaa cccggcttca ggggctaaag
      661 cccacgatga acatcagagg ctcatggcgg ggctgaagcc ccgcccttcc gttcttctcc
      721 gcgccgggcc aaaaaaaaat gcccgggacc ggcgacttga accggcctcg ggcatgcaga
      781 aatcgcccta cgattacctc aggccgggcc tcgcctccct gtccccgcgc ccctcgcagg
      841 tccagcgaac cgataccgca tgggaagttc cctgccctgg ccgagagttt ccggcggggt
      901 cagcgaggca gctacttccc ggttccgatt tggacggaag ctccattgcg ttgcgctttt
      961 gtccgaattg actagtaaat gcagacgttg agtttgtcaa gcaaagagtg gacctttgca
     1021 cgatttattt tgcggggaac agcatatttt tcgggtcgcg accgcgatgg cggcaatctg
     1081 cggcatagac gtcccaatac gtgccgcgaa tgtagtcggc gggggtgtat ccgagcgcgc
     1141 gagccacgcg atcgatcgcc ctgggctggc cttcgagttc gaggaatacg cctactggcg
     1201 tttcatccag gtccatgtgg aggccgcgca ggcggaaact ggtgcggtac ttctcgtagc
     1261 ggaagctcgg cttgaaaccc agggccgcaa gcgaggcggg ccaggagcgc gatgggtcgg
     1321 caacaacctc gcgttcggcc cgctctttgt atcgtgggct cttcctggtg ccgtacgctt
     1381 gctgcagggg cgctttggag gtcagtagga cacggcggat gcgtccgttg gaagcgccag
     1441 gcttggctga ggcttccatt cggacgcgca agagccatct gcggcgtcgc aggtcggact
     1501 ggggcgtgtc gtagagcgcg ttttgttcga agacgcgacc gcgggaggcg gcgccgagcg
     1561 actttatcgc acgcgccgtg gcgtggacgt ccgtgacccg tagcttaacc tcgatctccg
     1621 agttcgtttt tcgcgattga ggcacggcgc agtataaatc atgtgccggt tacggcggca
     1681 ccctcggtgt tgccaaacgc gc
//
LOCUS       DATAQL010001428         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_908669, whole genome shotgun sequence.
ACCESSION   DATAQL010001428 DATAQL010000000
VERSION     DATAQL010001428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908669"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1249
                     /locus_tag="VHS97_24665"
     CDS             347..1249
                     /locus_tag="VHS97_24665"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX3451469.1"
                     /translation="MLPEQKSAPGRLLSLDAYRGFVMLAMASRGAVMIGGLARSDDTV
                     SSHLLQILKQQLDHVSWRGCSFWDLIQPSFMFMVGLAKPFSYARRRARGDRWIQMFGH
                     AVQRSLMLIVLAIFLASNGSRSTQFSFVNVLGQIGLGYPFVFLLLGRRPALQLAAAIG
                     ILLGAWLLFDSYPLPGEDFPAAKYGANDPQLVMTGSFAHWNKNANIAAAFDRWFLNQF
                     PHPTDHPFVFKEGGYATLNMNSIAIYLMAQLMKPFVGAPLRTHFGHELFSGPYGPLIR
                     GSCTLLVFWLICLWLYRQKIFIKI"
     gene            1296..3122
                     /locus_tag="VHS97_24670"
     CDS             1296..3122
                     /locus_tag="VHS97_24670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451470.1"
                     /translation="MPRTAWLRIVRLTVLGMACTLGLFGIMLLPDLIRLIPERIRAWW
                     VEALSLVCLIVYAIARRLASVKSGGRPSTELARAGLRRLDFFTDRALKDGLALALALL
                     CLGLVLVWVPHYLTWPWSRDQETFAVLAQSWDQGILPYRDIRAYNFPGATYISWVLGK
                     VFGWGHTVPLYAFDAGCIVLLGAALSAWSRRRMGGAIPGLIGYLAFLTLYLNLRYQDV
                     VERDWHTALLMCLALMVLQAWPGRFAQLASALAAALALTIRPHAVLFLPALLWEVAQG
                     PDALGENRSKSKRIRAVAHWCMWMAIFVTIAFAPLVLAGIAGDFVRGLRIVAYGGPYS
                     KTTPADMMRVFVDQLTSWKTDVPLIATLLAAARPREALSRMAGSWSLALLAALLYKPI
                     HPLHHGYLIIPIALVSSITWAFAVSWLLSLHRLGRPVLVLAIALLVYEIMPARPYMCS
                     VTNSLSALPPLFLGETPPTPPLGCLWAFGVRSDRWDNYREVLSYIGKETSPQTLVANV
                     LNRFPYESLNGPTGRLSPFLAESGICWMSHVDIDLDPEFAESLIAAADSVVVWSPSES
                     KVEPRLKLERGIAVTRQYYEPAARFGRIEVWKRKAPGGRHSP"
     gene            complement(3607..>4760)
                     /locus_tag="VHS97_24675"
     CDS             complement(3607..>4760)
                     /locus_tag="VHS97_24675"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3451471.1"
                     /translation="SWRVIGRSSSRTSQTMSGQKAHEMPADVTIAYFDLFPDGRVVAN
                     KSFGYRFDPSGMFPRLPNDAEQAKSGWVSKNERDSKTTEFKHSSTDGSHGDEWAFDAV
                     DHGIWDKIYLMSSHARNFFDLKKGLVKRIENENTQDYGFHGKGTGTVTLTAMEEQNPD
                     QIKALDHESAHYFAANQKYEDLTTEAGKDAEHVKDLLAKAETTLKDAKDSLTLPDLRE
                     QIDVQLKKHKQMESYYTEEAKNRSAVLGKPAADWETKDLEGKTHSLKGYNGKVVILDF
                     WYRGCGWCIRAMPQMKELAEDFKGQPVAVLGMNTDSEEKDAKFVVDEMKLNYPVLKAE
                     GLPEKYHVRGFPTLIIVDQTGKVADVHVGYSSTLREEVAKAVKRLLARQ"
BASE COUNT          796 a         1419 c         1413 g         1132 t
ORIGIN      
        1 cgaacataaa ttcgtgtaca tgtttaccag cggcaacaaa atggcccgag cgtcgtggaa
       61 atggtgtcgg gcgggggacc ttccagcgcg acgttaaacg gcgtacctgc gaaatcgtct
      121 gttcgatcaa ggggggcgaa ttccgcaaaa atcatcggca ctggcgcgag ccgatctggc
      181 atgggaatcc accgccgtgg gcgcccggag accacgagcg aacggtcacg ggggtccgtc
      241 ctattcgacc caccgtctga tcacatacaa tcagcctttc acggacttgt ttctccgcga
      301 ttagaccctg ccgggttcac tggccttcct cagagggcaa acacccatgt tgcccgagca
      361 gaaatcggct ccagggcggc tcctttccct cgatgcctac cgcggttttg tcatgctcgc
      421 gatggcttca aggggcgctg tcatgatcgg cggcctcgcc aggtctgatg acacggtgtc
      481 gtcgcatctg cttcagattc tcaagcagca gctcgatcac gtgtcttggc gaggctgttc
      541 cttctgggac ttgattcagc cctcattcat gttcatggtc ggtttggcaa agccgttctc
      601 ctacgccagg cggcgcgccc gcggcgatcg ttggattcag atgttcggcc acgcagttca
      661 gcgctcgctg atgttgatcg tgctggcgat tttcctcgcg tccaacggca gccgctccac
      721 tcaattctcg ttcgtcaacg tgctcggaca gatcggtctg ggatacccgt tcgttttcct
      781 gctgctcggc aggcggcccg cgctgcagct tgctgccgcg attgggatac tgctcggagc
      841 ctggctgctc ttcgactctt atccgctgcc gggcgaggac tttcccgccg ccaaatacgg
      901 cgctaacgat ccccagcttg tgatgaccgg ctctttcgcc cactggaaca agaacgccaa
      961 tatcgccgcc gcgttcgacc gttggttctt gaatcagttc ccccacccaa ccgatcatcc
     1021 tttcgtgttc aaagagggag gatacgcgac cctgaacatg aactcgatcg ccatctacct
     1081 gatggcccag ctcatgaagc cgttcgtcgg cgcaccgttg cggacccatt tcggccatga
     1141 gctattcagt gggccgtatg gacctctcat tcgagggtcc tgcacgcttc tggtattctg
     1201 gttgatctgc ctgtggctct atcgtcagaa gatcttcatc aagatttgag cgtcgagttc
     1261 ccctcgccgc cggctttgcg agacactgaa tactcatgcc gcgaacagcg tggttgcgaa
     1321 tcgttcgcct gaccgtcctg ggtatggcgt gcacgctcgg actgttcgga atcatgctac
     1381 tgcccgattt gatccgactg atccccgagc ggattcgtgc ttggtgggtc gaggctctct
     1441 cgctggtctg tctcatcgtc tatgcgatcg cgcggcgatt ggcgtcggtc aaatccgggg
     1501 gaagaccttc caccgagtta gcccgggctg gcttgagacg gcttgatttc ttcactgacc
     1561 gcgccttgaa ggatggtctg gcgctcgccc ttgcactgct ttgcctcggg ctcgtgcttg
     1621 tatgggttcc ccactacctg acgtggccgt ggagtcgcga ccaggagacg tttgccgtac
     1681 tcgcccaatc gtgggatcaa gggattcttc cgtaccgcga catccgggcg tacaactttc
     1741 cgggagcgac ttacatttcc tgggtgctcg ggaaggtctt tgggtgggga cacaccgttc
     1801 cactttacgc cttcgatgcg gggtgcatcg ttctcctggg cgcggccttg agcgcctgga
     1861 gccggaggag gatgggcggc gccatccctg ggttgatcgg ttatctcgcc ttcttgacat
     1921 tgtacctgaa tctgcgctac caagacgtcg tcgagcgtga ctggcacact gcgctcttga
     1981 tgtgcctggc gctcatggtc ctgcaggcct ggccgggacg ttttgctcag ttggcgtctg
     2041 ctctggcggc cgcgctcgcg ttgacaatcc gtccccatgc cgtgcttttc ttgcctgcat
     2101 tgctctggga ggttgcgcag ggaccagatg ccttgggcga aaaccggtcc aagagcaaga
     2161 ggattcgcgc cgtcgctcac tggtgcatgt ggatggctat tttcgtgacg attgcgttcg
     2221 cgccgctggt gttggcggga atcgccggcg acttcgttcg cggccttcgc atcgtcgcct
     2281 acggagggcc ctatagcaag accacgcccg cggacatgat gcgcgtgttc gtagatcagc
     2341 tcacctcgtg gaagactgac gttccgctga ttgcgacctt gttggcggcg gcccgtccgc
     2401 gtgaagcgtt gagccggatg gctgggtctt ggtcgttggc attgctggcc gcgctgcttt
     2461 acaagccgat ccaccccttg catcatgggt acctcattat tcccatcgct ctggtcagtt
     2521 cgatcacctg ggcattcgct gtgtcctggt tactctcatt gcaccggttg ggcaggcccg
     2581 tacttgttct ggccatcgcg ctcttggtgt atgaaatcat gccggcgcgc ccttatatgt
     2641 gcagtgtcac taacagccta tcggctctgc cccccctgtt cctcggagaa acaccgccca
     2701 cacccccgct cggctgtctc tgggcgtttg gagtacgaag cgaccgatgg gacaactatc
     2761 gcgaggtatt aagctacatc ggtaaagaga cgagcccgca gacgctcgtt gccaacgtcc
     2821 ttaaccgctt cccttatgag tcgctcaacg gtccgacagg ccgactctct cccttcctgg
     2881 ctgaatcggg gatctgctgg atgagccatg tcgacatcga cctcgaccca gaattcgcag
     2941 agtcgctgat cgccgccgcc gattcggtgg tggtctggtc gccgagcgag agcaaggtgg
     3001 aaccgcgtct gaagctcgaa cggggcatcg cggtgacccg gcagtactac gagccggcgg
     3061 cgcggttcgg tcggatcgag gtgtggaagc gcaaggcacc aggcgggagg cacagtcctt
     3121 gatccggaat gaatcacggt cttgatcgct gaacgcgaac agccgatacc gaagggagaa
     3181 aaagcccgtc ccaacgtgag gccaggcgct ggactggcgt gggcgctctt gtgccttctg
     3241 gtggcaaggt ggtttgagcg gcgtcgtttg gtcgagcggg tggacgagtg acgaggtgac
     3301 cgcaccccct cacgcaacgc gagctaagca gacgtaaaag aacatcactt gatgtgacaa
     3361 ttggagtgtc cttccccaac ggggagagga atgagcggcg agcgggagcg ccgttacgca
     3421 tgatacgcgt agttagatcg atgattggtg tcgaaaacct ttggttttat cttgtaagaa
     3481 ggggcttcgt tccccgatcg agattgccgg tcggagcccc gtcctacagc gaatcgcacc
     3541 actcggttct cgccgaaacc gtgcttaacg gcgccgcctg aggggaaact ctgcttgcag
     3601 ttctgctcac tgccgcgcca gaagccgttt caccgccttg gcgacttcct cgcggagcgt
     3661 ggacgagtag ccgacgtgca catcggctac cttgcccgtc tggtcgacga tgatcagtgt
     3721 ggggaacccg cgcacgtgat atttttcggg caatccctcg gccttcaaga ctgggtaatt
     3781 caacttcatt tcgtcgacga cgaacttggc atccttctcc tcgctgtcgg tgttcattcc
     3841 aaggacggcc accggttggc ccttgaagtc ttcggcgagt tctttcatct ggggcatggc
     3901 ccggatacac cagccgcagc ccctgtacca gaagtcgagg atgacgacct tgccgttgta
     3961 gcccttgagc gaatgtgtct tgccttcgag gtccttggtc tcccagtcgg cggcaggctt
     4021 gcccaggacg gcgctgcggt ttttggcttc ctcggtgtag tacgactcca tctgcttgtg
     4081 ctttttcaac tgcacgtcga tctgctcgcg caggtccggc agcgtgagcg agtcctttgc
     4141 gtctttcagc gtcgtctcag ccttggcaag caggtccttc acgtgctcgg catccttgcc
     4201 cgcctcggtc gtgaggtctt catatttttg attcgccgcg aagtagtgcg ctgactcgtg
     4261 gtcgagcgcc ttgatctggt cggggttctg ctcttccatg gctgtgagcg tcactgtccc
     4321 ggttcctttc ccgtgaaaac cgtagtcttg cgtgttctcg ttctcgatgc gcttgacgag
     4381 gcctttcttg agatcgaaga agttccgcgc gtgcgagctc atcaaataaa tcttgtccca
     4441 gattccgtga tcgacggcgt caaatgccca ttcatctccg tgcgatccgt cggtgctcga
     4501 gtgcttgaac tcggtggtct tgctgtcgcg ttcattcttc gacacccagc cgcttttggc
     4561 ctgttcggcg tcgttcggca gacgggggaa catgccagac gggtcgaacc ggtagccgaa
     4621 cgatttgttg gcgacaacgc gtccatcggg aaagaggtcg aaataggcga tcgtcacatc
     4681 cgctggcatc tcgtgcgcct tctgtccgct catagtttga gacgtccggc tgctcgagcg
     4741 gccgatgacg cgccagcttc
//
LOCUS       DATAQL010001429         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5185966, whole genome shotgun sequence.
ACCESSION   DATAQL010001429 DATAQL010000000
VERSION     DATAQL010001429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5185966"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VHS97_24680"
     CDS             complement(<1..236)
                     /locus_tag="VHS97_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HEX3451472.1"
                     /translation="MAALLAQMNPNPWTTYVWLMMGAIGFFALIASIFLAKYFNLWIQ
                     AKMTLANVTIWELVGMSFRKVNPNIIVRSKIMAY"
     gene            complement(521..2746)
                     /locus_tag="VHS97_24685"
     CDS             complement(521..2746)
                     /locus_tag="VHS97_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX3451473.1"
                     /translation="MIRPRAARLQANFGPFLARAWPVLLLPLLFVVGAPAQEAGARGD
                     GAPGQFFTITEPITHDTLARVRAATRQLVDRNASAEQGTRPILVFEFLSGESAPGASE
                     FGACYDLANLISKELAGARLTVAYVPQPVRGYAVLPAIACTEIVMGSSASLGPITPEN
                     LSADAALREPVRFLAMRKTRDPDLLLGMLDRDADLRLVHTADKTVHYVLADNMKAFRQ
                     THEVADDKPAWEGGQRGVLTAARAREEGFCKRTADSPAELASIYQIGGQSAVDDPTLG
                     QAIRPAWIKIDGPLDARLVFYLTRSLEQARQEKVNLLILEINSTGGMETEGDNLADLV
                     SSIKDMKTVAYIDDRATGVAALLPLACRDIVFSKKARMGDVRQVIGGGGGHLVDLTEL
                     MRSSLAKKAAMLARQKGHPEAVALAMVDPDTEVIEAKDSKTGAARLITRSELEAENGR
                     FQALQTRKEAGTLLTVRADDATSYGLGQMVADDDELKGLYGLRGRAIRVDGPGWVDSL
                     VTILTDPYVSWLLLFIGAFMLVIELKLPGIGLPAIISALAFLLFFWSHYLSGTADQLE
                     IILFLIGLVSLAVEIFVFPGFGVFGMSGILLMLCSIVLASHTFIWPTQDYEYQELGHT
                     LLQVTGMLVAVGGCAVVLARYFPSLPFFNRLVLKPEPWTRVEPEDSLGRPIAEGYESL
                     AFLVGETGRTTSPLRPTGKAQFGGLVIDVTADRAFVETDSLVEVVDVQGPRVIVKRVG
                     A"
     gene            complement(3160..>4038)
                     /locus_tag="VHS97_24690"
     CDS             complement(3160..>4038)
                     /locus_tag="VHS97_24690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451474.1"
                     /translation="TLAVAQDRGKVAQPAQRQQQPAAAQPAMNMDQLLQLWAGQSAKL
                     ETLELDIYRIDEQKAWGDVEHYLGHAAFKAPQLAFLDFRKVKMQEKADPKDKQKKVVV
                     PVMKNNKIDSGPYETIVCTGDEVWHYRYDVKKIFVFPLDKNQRKRAIEEGPLPFLFNM
                     NVADAQRRYQMELVGEDQKKGYMVKVVPQLKEDQESFSVAWVQLDKKFLLPTRIYLLA
                     PDKKSSKDFQLSTIQANKAVNKQFFVGVNPGKGWKVERNPGGPAPAPADAKGLRRQPN
                     GQPPQRAAAPADGQPR"
BASE COUNT          789 a         1197 c         1210 g          842 t
ORIGIN      
        1 tggtaggcca tgatcttgga acgaacgatg atgttgggat taaccttgcg aaatgacatt
       61 ccgaccagtt cccagatcgt gacgttggcc aaagtcatct tggcctggat ccagaggttg
      121 aagtatttcg ccaggaaaat gctcgcgatc aatgcgaaga acccgatcgc gcccatcatc
      181 aaccagacgt aggtcgtcca ggggtttggg ttcatctgag cgagcaatgc cgccatcttc
      241 ttgcatcctt agttgttggt gttcgacccg gcttgtcgga gggaaagaga ccgacaaggt
      301 cgccaagctt ttgaaaaagg ggagacaacc cgcctccgtc gagcctcgat aaggttcggt
      361 ccggttgtat tcgtgttgtg tttgttagtt acagtttaat cgaagaccgc gctcgttcgc
      421 caacccctgg caccatccta gccacgttcc gccattcgag gggccgatct tgcccgcgtc
      481 gccgggggcc ggtcgagctc gatccctcgc acggagtcag tcaggcgccg actctcttga
      541 cgatgacccg cgggccctgc acgtcgacga cctcgaccaa actatccgtc tctacaaagg
      601 cgcggtcggc cgtcacgtcg atgacgagac ctccgaactg ggctttgccg gtcggccgca
      661 gcggcgaggt ggttctgccg gtctctccca ccaggaaggc gagcgattcg tacccttccg
      721 cgatcggcct gcccagagaa tcctctggct cgacgcgcgt ccagggctcg ggtttcaaga
      781 ccaggcggtt gaagaacggc aacgagggaa aataacgagc caagacgacc gcgcaacctc
      841 ccactgcaac cagcattccg gtcacttgca gcaatgtatg ccccagctct tgatactcat
      901 aatcctgggt gggccagatg aaggtatgac tggcgaggac tatgctgcaa agcatgagca
      961 ggatgccgct cattccgaag acgccgaagc ccgggaatac gaaaatctcc acagccaaac
     1021 tgacgagccc aatcaaaaac aagatgattt cgagctggtc ggccgtaccg ctcaggtagt
     1081 ggctccagaa gaagagcagg aatgccagtg ccgagatgat tgccggcagg ccgatgccgg
     1141 gcagcttgag ctcgatgacc agcatgaagg cgccgataaa gagcaggagc cagctcacgt
     1201 agggatcggt gagaatcgtc acgagcgaat cgacccaccc tgggccgtcg acccggatcg
     1261 cgcggccgcg cagaccgtag aggcccttga gctcgtcgtc atcggccacc atctggccca
     1321 gaccgtacga tgtggcatcg tcggcccgca ctgtcagcaa ggttccggcc tccttgcggg
     1381 tctgaagtgc ctggaatcgg ccattttccg cttcgagctc tgaccgagtg atcaggcgag
     1441 cggcgccagt ttttgaatcc ttggcttcga tgacctcggt gtcgggatcc accatcgcca
     1501 gagccacggc ctcgggatgc cccttttgcc gcgccagcat tgcggctttt tttgccaggc
     1561 tcgagcgcat cagttcagtc aggtcgacca ggtgtccgcc gccaccgccg atcacttgtc
     1621 ggacgtctcc catcctcgcc tttttactga acacgatgtc acggcacgcc aggggcaaga
     1681 gggccgccac cccggtcgcg cggtcgtcaa tgtaggcgac cgttttcatg tccttgatgg
     1741 atgagaccag atcggcgaga ttgtctccct cggtttccat gccgccggtg ctgttgatct
     1801 ccaggatcag caggttaact ttctcttgcc gggcctgctc gaggctccgc gtcaggtaga
     1861 aaaccaatct ggcgtcgagc ggcccgtcga tcttgatcca ggccgggcga atcgcctgac
     1921 ccagggtcgg gtcgtcgacc gcggattgcc cgccgatctg ataaatgctg gcgagctcgg
     1981 cggggctatc ggccgtgcgc ttgcaaaaac cctcctcgcg tgcccgcgcc gcagtcaaga
     2041 cgccacgctg gcctccttcc catgcgggct tgtcgtcggc cacttcgtgg gtctgacgaa
     2101 aggccttcat attgtcggcc aaaacgtaat gaactgtttt atccgccgtg tgcacaagcc
     2161 gaaggtcggc gtcgcggtcc agcatcccca agaggagatc ggggtcgcgc gtcttgcgca
     2221 tcgccaagaa tcgtaccggc tcgcgcaggg cggcatcagc gctcaggttt tcgggagtga
     2281 tcgggcctaa cgaagctgac gaccccatca cgatctcggt gcacgcgatc gcgggtaaca
     2341 cggcataccc gcgcaccggc tgcggcacat aggccaccgt cagcctggcc cccgcaagtt
     2401 ctttcgagat caggtttgcc aggtcgtagc aggcaccgaa ttcgctggcc ccgggggcag
     2461 actctccgga caggaactcg aagaccagaa tcggtcgtgt tccttgctcg gccgacgcat
     2521 tgcggtcgac gagctgccgt gtggcggccc gaacgcgggc gagcgtgtcg tgcgtgatcg
     2581 gctcggtgat cgtgaaaaac tggcccggtg ccccatcgcc acgggcgccc gcttcttgag
     2641 ccggcgctcc aaccacgaag agaagcggca ataagagaac gggccaagcc ctggccagaa
     2701 agggcccaaa attggcttga agacgcgcag cacgggggcg gatcatagca gccagactcc
     2761 ctccgagagt cgaacgcgcc tctgttctcc ctcgcggcgg cacgacaaac cgagcttcaa
     2821 agccgtgcga ttccgcagaa ctccttcaca gagaaatcaa acactttgat tatagcgtgc
     2881 caaagagggc gcggaccgaa ccaaccagat cgttctcgtc aagattataa gctggcggat
     2941 agggagggtc aaacatcctc gaacgcgaac ggcgcggctg gaggcggcgc agcccggccc
     3001 ggcggcagaa ttgaatgaat agccgaatcg tgtaattttt accttgttcg gagcaattcc
     3061 tgaatatttg attgagtata tgacggggtg ttcgtcaccg gcgtaggggt gccgccggga
     3121 ttgcacaggg ctatcgactg cgaggcgccc gacagagggc tagcggggtt ggccgtcggc
     3181 cggagccgca gcgcgttggg ggggctggcc attgggctgg cgccgtagac ctttcgcgtc
     3241 ggctggtgcc ggtgccggtc cgcccggatt tcgttcgacc ttccagccct ttccggggtt
     3301 gacgcccacg aaaaattgtt tattcacagc cttattcgcc tggatcgttg atagctggaa
     3361 gtctttgctg ctcttcttgt cgggggcgag cagatagatt cgggtcggca aaaggaactt
     3421 tttatcgagc tggacccatg caacgctgaa gctttcttgg tcttccttga gctgaggcac
     3481 taccttgacc atgtagccct ttttttgatc ttcccctacg agttccattt ggtagcgccg
     3541 ctgggcgtcg gcaacgttca tgttgaacaa gaagggcagt ggtccctcct cgatcgcgcg
     3601 cttacgttga tttttatcga gcggaaaaac gaagattttc ttgacatcgt agcgataatg
     3661 ccagacttcg tcgccggtgc aaacgatggt ttcgtagggg ccagagtcga tcttgttgtt
     3721 tttcataact ggcacgacca ccttcttttg cttgtccttg ggatcggcct tttcctgcat
     3781 tttgactttg cgaaagtcga gaaacgcgag ttgcggagct ttaaacgcag cgtgccccag
     3841 ataatgttcc acgtcgcccc aggctttttg ctcatcgatc cggtagatgt cgagctcaag
     3901 agtctcgagc ttcgcgcttt gcccggccca aagctgcagg agctggtcca tattcatggc
     3961 cggctgggct gccgccggtt gttgctgccg ttgcgccggc tgagcaactt tgccccgatc
     4021 ctgggcgacg gcgagcgt
//
LOCUS       DATAQL010001430         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_300008, whole genome shotgun sequence.
ACCESSION   DATAQL010001430 DATAQL010000000
VERSION     DATAQL010001430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300008"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VHS97_24695"
     CDS             complement(<1..881)
                     /locus_tag="VHS97_24695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245755.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX3451475.1"
                     /translation="MATDVLDEPEIQTEAKQATWTLNFGPQHPATHTTLRLILELDGE
                     RIIKATPDIGYLHSGFEKLGEHLNFNQYVTVVDRKNYISPPLNEVAWHHAVEKLLDIE
                     LTPRCQYIRVIISELARISDHLLCTGAAALDLGAFTAFLFAFNLREQIYDVYEEMSGY
                     RFHPGYTRVGGLLYDFNDRVIEKVRLVLASFPQVYADMEKLLFRNRIFLDRVRGVGVL
                     SKADAIAYSCTGPIARASGVTYDLRKDEPYLAYPDFDFEVPYTVEGDCWARFIVRMEE
                     MRQSHRILQQALEKLPGG"
     gene            complement(921..1454)
                     /locus_tag="VHS97_24700"
     CDS             complement(921..1454)
                     /locus_tag="VHS97_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX3451476.1"
                     /translation="MSTAAASSALQHSATLGNLTKVFGDGVYSTSRFRDNLRLFVPGS
                     RLIELLELLKNTCGFNLLSELGATDYLGYPGRTRARFEVHYVLKNLDTAECLVVKCGV
                     DDPDPKVPSAVPIWPGADWMEREVFDMFGIRFDGHPDLRRILMPEEFAAYPLRKDYPL
                     RGRGERHNFPRLSRAES"
     gene            complement(1526..2239)
                     /gene="nuoB"
                     /locus_tag="VHS97_24705"
     CDS             complement(1526..2239)
                     /gene="nuoB"
                     /locus_tag="VHS97_24705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX3451477.1"
                     /translation="MAIGTPGSSATPATHRAPAGPTLSVQPAGVPELPENVHVTTLDS
                     VVNWCRKYSLWPMPFATACCGIELMAVGASRFDIARFGAEVMRFSPRQCDLMIVAGRV
                     AMKMMPVLQRIWLQMPEPKWCFSMGACASTGGIFDTYAVVQGIDRFIPVDVYIPGCPP
                     RPEQILRALLDIQEKIQKGGSMRGGNGLPEMLERERYMDEVRKSPLGLATAGERGDED
                     RFGYRLPGGPARLGASESS"
     gene            complement(2230..2628)
                     /gene="ndhC"
                     /locus_tag="VHS97_24710"
     CDS             complement(2230..2628)
                     /gene="ndhC"
                     /locus_tag="VHS97_24710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX3451478.1"
                     /translation="MGGEYTPVFLLLLAAIAIGGGMMIASAFLAPYKQTKIKQMPYES
                     GMDPIGDARQRFDVRFYLVAIVFLLFDVELLFLYPWAVAQWSAGLSGDGAAGIPADFR
                     GWVFWEIMVFIVILAAAFAYAWRKGVFEWR"
BASE COUNT          653 a          929 c          954 g          588 t
ORIGIN      
        1 ccgccgggga gtttctcgag cgcttgctgg aggatccggt gactctgccg catctcctcc
       61 atccgcacga tgaatctcgc ccagcaatcg ccttcgacgg tataagggac ctcgaagtcg
      121 aaatcggggt aggcgagata gggctcatcc ttgcgcaggt cgtaagtgac gccgcttgcc
      181 cgtgcgatcg gtccggtgca ggaataggcg atagcgtccg ccttggacag cacaccgacg
      241 ccccgcaccc gatcgaggaa gatgcgattg cggaacaaga gcttctccat gtcggcgtag
      301 acctggggga agctggccag caccaagcgg actttctcga tcacccggtc gttgaagtcg
      361 tagagcaggc cgccgactcg ggtatagccg gggtggaacc ggtagcccga catctcttcg
      421 tagacgtcgt aaatctgttc cctgaggttg aaagcgaaca agaaggcggt gaatgccccg
      481 aggtcgagcg cggcggcgcc cgtgcagagc aagtgatcgc ttatccgcgc cagctcggag
      541 atgattacac ggatgtactg gcaccggggg gtcagctcga tatccagcag cttctcaacg
      601 gcgtggtgcc aggccacctc gttcaggggg gggctgatgt agtttttgcg gtcgaccacc
      661 gttacgtact gattgaaatt gaggtgttcg ccgagtttct cgaaccccga atggagatag
      721 ccgatatcgg gcgtggcctt gatgatccgc tcgccatcga gctctaagat cagccgcagc
      781 gtcgtatgcg tggcgggatg ctgaggcccg aaattcaacg tccaagtggc ctgctttgcc
      841 tcggtctgga tctccggctc gtcgagtacg tcggtcgcca tcgcgtcatc ctgcctgact
      901 ggtgaagagc gggggccgcg tcatgattcg gcccgcgaca gccgcggaaa attatgccgt
      961 tctccgcgcc cccggagtgg atagtccttg cgcagcggat acgcggcgaa ctcttcgggc
     1021 atgaggatgc gccgcaagtc gggatgtccg tcgaatcgga tgccgaacat gtcaaaaact
     1081 tcccgctcca tccaatcggc gcccggccag atcggcacgg ccgacgggac tttgggatcg
     1141 gggtcgtcga ccccacactt gaccaccagg cattcggccg tgtcgaggtt tttgagaacg
     1201 tagtgaacct caaaacgagc ccgggtccgg ccggggtagc cgagataatc ggtcgcaccc
     1261 agctcggaga gaagattgaa gccgcacgtg ttcttgagta attcgagcag ctcgatcagc
     1321 ctggaaccgg gcacgaaaag ccgcaggttg tctcgaaacc gtgacgtgct ataaacgccg
     1381 tcgccgaaaa ccttggtcaa gtttcccagc gtggcggagt gctgaagagc ggaagatgcc
     1441 gcggcggtgc tcattgggca gtggctattt ttcagtttac cgttacacga gaaaacacga
     1501 tttcaagacg atcgttgccg gtgcgtcagc tcgattcact ggccccgagt cgggccggtc
     1561 ctcccggcag gcggtagccg aaccggtctt cgtcgccacg ttcgccggcg gtggccaggc
     1621 cgagaggcga ctttcgcacc tcgtccatgt atcgttcgcg ctcgagcatc tcgggaaggc
     1681 cgtttccacc acgcatcgag cctccctttt ggatcttttc ctgaatgtcc aagagggcac
     1741 gcagaatctg ttcgggccgg ggaggacagc ccgggatata aacgtcgacc ggaatgaaac
     1801 ggtcgatacc ctgcaccacg gcatacgtat caaagatccc gccggtcgac gcacaggctc
     1861 ccatgctgaa gcaccacttg ggctcgggca tctgcagcca gatgcgctgc aagaccggca
     1921 tcatcttcat cgcgacgcga ccggcgacga tcatcaagtc gcattggcgc gggctaaatc
     1981 gcatgacttc ggcgccaaac cgggcgatgt cgaatcggct ggcacccacg gccatcagct
     2041 cgatgccgca gcatgccgtg gcaaacggca ttggccagag gctgtacttg cgacaccagt
     2101 tgacgaccga gtcaagcgtt gtcacgtgca cattttcggg cagctcgggc actccggcag
     2161 gctgaacgga cagagtcggc ccggccggtg ccctgtgggt ggcgggcgtg gccgaagagc
     2221 cgggtgtacc tatcgccatt cgaagacccc ttttcgccat gcataagcga aggccgcggc
     2281 caggatcacg atgaagacca tgatttccca gaacacccag ccacgaaaat cggcaggtat
     2341 tcccgccgcc ccgtcgccag acaagcccgc gctccactga gcgactgccc agggataaag
     2401 aaacaaaagc tcgacatcga aaagcaggaa gacaatcgcg accagataga aacgcacgtc
     2461 aaatcgctgc cgtgcgtcgc cgatcgggtc catccccgat tcgtacggca tctgcttgat
     2521 ttttgtctgc ttatagggag ccaagaaagc gctggcgatc atcatgcccc cgccgattgc
     2581 aatcgctgcc aagagtaaga ggaaaaccgg cgtgtattcc ccgcccatcg atcgctactc
     2641 ctacctttgg cccgcccaat ccgagagacc tttgtgaagg gtttcacaaa gtcggatcca
     2701 aaaaaaaccg ggcttgcgcc aggatcgagt tcaatacgcg cgatcgcagg actgtccgcc
     2761 agccattgcg atctcgatca gtctcattag atggccgccc gcccgacgaa acaacgagct
     2821 caagctccgg cggcccaacg acgcccctat tgtaaggttt gagaatccag cgtcaacgag
     2881 ttagcgattt ccggtacccg gcgattctcg ggcttaatta tcggatgggg gaaccaaagg
     2941 ggtcgtcgta gacggtgtgc gacggttcgc cccggttgtt ggtcggctgg gcgtccggga
     3001 gggaaagccc ggagccgttg catcgtcatc catgttcaat cgaagcgagc gattgagaac
     3061 atcagaagga ctgcgccggg ttcgacttcc tcgggtccct agcccaggga agaggggcgg
     3121 agcc
//
LOCUS       DATAQL010001431         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2284747, whole genome shotgun sequence.
ACCESSION   DATAQL010001431 DATAQL010000000
VERSION     DATAQL010001431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284747"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..1016
                     /locus_tag="VHS97_24715"
                     /pseudo
     CDS             369..1016
                     /locus_tag="VHS97_24715"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
     gene            complement(1115..2263)
                     /locus_tag="VHS97_24720"
     CDS             complement(1115..2263)
                     /locus_tag="VHS97_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX3451479.1"
                     /translation="MATKLVNVENIGSYFESLSDPRHTRNRKHLLADILVITVCGILC
                     GSDGPTAIHRWADNRLDWLKQFLPLPNGIPSRDCIRRLLMMLKPEAFQKCFQEWTTHV
                     IQVDDSGSKRLVAIDGKTCRRSHDAAHQLGSLHIVSAWASEEGVALGQVATEAKSNEI
                     TAIPELLKQIELKDTLITIDAMGCQKEIAHDIVDGGGDFVISLKDNQPKLREAVETYF
                     AKHLELDLEDLEYRSHETSESSHGRLDERSYFLTKIPPDFALKKEWPWIKAIGYSLRV
                     TQHADGSETDETRYYILSRYLSGKRFCEAVRGHWAIESMHWVLDVNFREDDSRTRERT
                     LGNNLSWLRRFSVSLLKRHPVNDSLRGKMIRCSLNTEFLAEVLTIQRD"
BASE COUNT          525 a          788 c          757 g          516 t
ORIGIN      
        1 tcaccgcctc aacagccctg gcgaccctcg cccctacgac gatctcgcag gcgcagacga
       61 ccaaacggct caaagtgatc atcccgatga cgaatccgga ccagctttcg gagctgcagg
      121 cggctgcgcc cggggcagag ctggtccagt gccggattga ggacgagacg ctcgagcgtg
      181 tcatcgacgc cgacgcgttg tatggcttta tcacaccgcg ggtgatccgc gccggcaagt
      241 cgcttcggtg ggttcaacag cccagcgccg gggtcgagca tctgatgaag atccctgaac
      301 tcgtgcaaag cgacatcata ctgaccaaca tgcaacgggc ctatgccccg gagattgccg
      361 atcaggcaat ggggtacctg gtcgcgttca cccgcagcct ggctcacttc gtccgcgcgc
      421 agccgagcca agagtggcgg tccagcggac ggggggttgc tctcgatgag ctgatgggta
      481 agacgctctt ggtcgtcggc ctgggcggca tcggtagcga gatctcgcgc cgcgccgcgg
      541 cattcggcat gcgggtcgtg gccacagacc caaaggtcct gtaacggccg ccgtgcgtcg
      601 acgaactgca caaaccgtcc gcgttccacg ccctgctgcc tcaggccgac gtcgtcgcca
      661 gcgccgtgcc cctcacgaac gtgtcgagga agatgatcgg cgccaaggag tttggaatga
      721 tgaaacctgg cgtcatcctg atcaatgttt ctcggggtgg agtggtcgat acccaggccc
      781 tgatcgatgc cctggatcgc aagcaggttg ccgccgcggg gctcgacgtg accgacccag
      841 agccgctgcc caagggacac gcgctctggt cgcgaaacgt gatcatcacg ccccacagcg
      901 cgggccagtc gcccggcggc gaacgacgaa agcacgaagt cctgtgtgag aacctacgcc
      961 ggttcgcgtc gggcgagatg ctgctgaaca tcgtcgacaa gagcgtcggc tattgatttg
     1021 tcgtcacttc aggccgtagc caaacgctga aggtagtacg gggagccgat tcgagacagg
     1081 agcgcctcgt cggtctgatc agggtcagcg catatcaatc tcgttgaata gtcagtactt
     1141 cggcgagaaa ctcggtattc aaactgcacc gaatcatctt gccgcggaga ctgtcgttga
     1201 ccgggtgacg cttcagcagg ctcacggaga accgtcgtag ccagctcaaa ttgttgccca
     1261 gtgttcgctc gcgtgtgcgg ctgtcgtctt cgcggaagtt cacatccaat acccagtgca
     1321 tcgattcgat tgcccaatgc ccacgaaccg cttcgcagaa tcgctttccg ctgagataac
     1381 ggctgaggat gtagtagcgg gtctcatcag tttcgctgcc atcagcgtgt tgtgtaaccc
     1441 gcagcgaata gccaatagcc ttgatccatg gccactcctt cttgagcgcg aagtcgggtg
     1501 gaatcttggt caaaaagtat gaacgctcgt cgagtcggcc atgagacgac tcgctcgttt
     1561 catggctccg atactcgagg tcttccaggt caagctcgag atgtttcgcg aaataggttt
     1621 cgacggcctc gcggagcttg ggttggttat ccttgaggct gatcacgaaa tcgccgcccc
     1681 cgtcgacaat gtcgtgtgca atctctttct ggcagcccat ggcatcgatc gtgatcaacg
     1741 tgtccttgag ctcgatctgc ttcaataact cgggaatcgc cgtgatttcg ttggatttcg
     1801 cctcggttgc cacctgaccc aacgcgaccc cttcctcgct cgcccaggcg ctgacgatat
     1861 gcagcgatcc cagttggtgg gccgcatcat gcgacctccg gcaagtcttg ccatcgatcg
     1921 cgaccagccg cttggaaccg ctgtcatcga cctggatgac atgagttgtc cattcctgaa
     1981 agcacttctg aaatgcctcg ggcttgagca tcatgagcaa acgacgaata caatcgcgtg
     2041 acgggattcc gttgggcaga ggcagaaact gttttagcca atcgaggcga ttgtcggccc
     2101 agcgatggat ggccgtcgga ccatcgctac cacatagtat cccgcacacc gtgatcacca
     2161 aaatatccgc cagcaaatgc tttcgatttc gggtgtgtcg gggatcggag agtgactcaa
     2221 aatacgagcc aatgttctca acgtttacca atttcgtggc catggaacac atcttggtat
     2281 gaggcgggca gacgttccgc gcgacccaca gccgccatag aatcggaaca ccgccattaa
     2341 ccgagatgta acatgtttcg atcgatcgcg ctagtctttg tagattacct agctatgcgc
     2401 gcgggccctg gccacgaccc cgcacctggt gaacgactcg atgacgaccc tcgccggccg
     2461 catcggccgt ctgaaaagca agcacgcgca cgccgaaagc ccgcgctcgc gcacccgccg
     2521 ccagatcgcc aacgtgcggg tctggcaggg gcccggcgac ctgttcagcc ggagcgtctt
     2581 cgacgt
//
LOCUS       DATAQL010001432         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4420479, whole genome shotgun sequence.
ACCESSION   DATAQL010001432 DATAQL010000000
VERSION     DATAQL010001432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4420479"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..943
                     /locus_tag="VHS97_24725"
     CDS             11..943
                     /locus_tag="VHS97_24725"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX3451480.1"
                     /translation="MAYQMTFAIITVALVAGSVADRMRFSAFVWFAILWLPIVYVPVA
                     HWGWGNGWLMKLGILDFASGTVVHLNAGVAGLVAAYMVGKRAGYGGENFAPHDLSLAV
                     IGTGLLWVGWFGFNGGSALAAGSRAAFAIVATHLAASAGALTWTLLEWWTRGKPSVLG
                     MISGAVAGLGTITPASGFVLPWHGIVIGITAGAVCFWAWTKLKQALGYDDSLDVFGVH
                     GVGGAVGTLLTGVFATTALSITPEAPAGSPGLLDGNPGQVLIQLYGIAATITWSAFAT
                     FVLLKAIGLIVPLRVSKEMEMDGLDLTQHGEALQ"
     gene            1327..>1556
                     /locus_tag="VHS97_24730"
     CDS             1327..>1556
                     /locus_tag="VHS97_24730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX3451481.1"
                     /translation="MPLAVHPGRLLKRELEARNLSANRLALDLGVPSGRITDILNGRR
                     SISADTAVRLGRYFGNRAQFWLDLQSQYDIAV"
BASE COUNT          260 a          465 c          503 g          328 t
ORIGIN      
        1 aatcctcttc atggcctacc agatgacatt tgccattatc acggtcgcgc tagtggcggg
       61 atcggttgcg gaccgaatgc ggttttcggc tttcgtgtgg tttgcaatcc tttggctccc
      121 gatcgtctac gttccggtcg cgcactgggg ctggggcaat ggttggctaa tgaagctcgg
      181 cattctcgat tttgcaagcg gcaccgtcgt ccacctcaat gcgggcgtcg ctggtctcgt
      241 cgccgcctac atggtcggca agcgtgcggg atatggcggc gagaatttcg cgccccacga
      301 tctgtcgctg gcggtgatcg gtaccgggct tctctgggtc ggctggttcg gattcaacgg
      361 cggttcggcg ttggccgcgg gctcgcgcgc ggccttcgcc atcgtggcga cgcacctggc
      421 cgcctcggcc ggcgcgttga cctggacact cctggaatgg tggacgcggg gaaagccgtc
      481 cgtgctcggc atgatttcag gcgcggtcgc aggcctcggc acgatcaccc cggcctccgg
      541 tttcgtgctg ccatggcacg ggatcgtgat cggcatcacg gcaggcgcgg tctgcttctg
      601 ggcctggacc aagctcaagc aggccctcgg ctatgatgat tcgctcgacg tgttcggggt
      661 gcacggcgtc ggcggggccg tcggcacgct tctcaccggc gttttcgcca ctacagcgtt
      721 gtcgatcact ccggaggcac cggcgggaag tcccggcctc ctcgacggca atcccggcca
      781 agtgctgatc cagctttacg ggattgcggc gaccatcacc tggtcggctt ttgcgacgtt
      841 cgtcctgctc aaggccattg ggctgatcgt gccgctgcgg gtgagcaagg agatggagat
      901 ggacggtctc gacctgaccc agcatggcga agcacttcag tagacgcgag cggagagttt
      961 tagcccggca ctagcgtatg gctgaccgca gccgaggtga cgatgtgacc gacatcctga
     1021 tccttggggg cctgggcaga tcgctcgcgt ggctaccgac ctttcctcaa agagaccgac
     1081 gcacgcctgg cgctctatct gcgcaatgcg aggcggtggt gaaggtggtc gaggcgagca
     1141 atatcgaaaa gtgaagggtc gtcgcccagt ctctacagaa ttcagtcctc tcaagagcat
     1201 tgggtatcac caactcaagg gtgaccgggc cggacactgg gcgttacggt caacgcacgc
     1261 tggcgcgtct cgttttcgtt caggagggca atgcgcataa tcgtcgatta gcataggggt
     1321 tagaccatgc cgctcgcagt gcatccaggc cgcctcctga agcgtgaact ggaggcacgc
     1381 aatctcagtg caaaccggct cgcgctcgat ctcggagtgc cctcgggccg tatcaccgat
     1441 atcctgaatg gtcgccgctc tattagcgcc gacacagcag tgcgactcgg ccgctatttt
     1501 ggaaatcgcg cacagttctg gcttgacctg cagagccaat acgacattgc cgtgat
//
LOCUS       DATAQL010001433         8278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_759938, whole genome shotgun sequence.
ACCESSION   DATAQL010001433 DATAQL010000000
VERSION     DATAQL010001433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759938"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VHS97_24735"
     CDS             <1..576
                     /locus_tag="VHS97_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451482.1"
                     /translation="EEAREAFRSRAYHAHPDRGGETKRFIRIRKAYEQILADLERQPV
                     PDADKNEQAVDKSAQSPHRDRRSKPPDPNWEPDFVLLDEPPRRTRPPRPRDPNWKPDL
                     ILLDDPASDRESTNSADVSWKTSRALFQRISDQSQQKDSTRQFGWIHAVGMLILFMMI
                     VLLSLTLFWTFSTSERQTEPVESPTYYPAVQ"
     gene            738..1712
                     /locus_tag="VHS97_24740"
     CDS             738..1712
                     /locus_tag="VHS97_24740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451483.1"
                     /translation="MPEQNDSVDRGDDDVAINPVDIQFDHAEYATPAPDGPSCGVCQS
                     PIEGEYYETGGKVCCPTCRHNIEAALRGGSRVARVLKSLILGSAASAVGAVLYYAILR
                     GTGYNIGLVAVVVGVIVGRAVRKGTGGRGGRFYQFLAVFLTYTAIVVMYVPLVLEGLS
                     KPAQNAPQAMPQVAKAGSDSAKSEARSKVADASKDGPSGPKADSPTDPAAAPEITAKD
                     RAVTPQEKTAVVDDGGAADVKEVPPVHKPAQIPLALLVATVFLIGLFYTVPVQIAVAA
                     PISGLIFAFALWEAWKANRGVHLSFNGPFRVSARGSSGPEPEVERDGR"
     gene            1702..2793
                     /locus_tag="VHS97_24745"
     CDS             1702..2793
                     /locus_tag="VHS97_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3451484.1"
                     /translation="MADELRSRVPLLAHNCTVCGSEIAPTLLSCPSCHRLVHGARLKE
                     LAADAEAAEKAGELSAALAFWNEAVSLLPAESRQYAAIADRIAMLGRRVEAGPKPPAQ
                     STPTVPHAKPTASSSSPWSGGALSGIAATLALLAWKFKFLAVLLLTKGKILLLGLTKA
                     STFLSMFVSMGMYWTVFGGWFAAGLVLSIYVHEMGHVVALSRYGIHAGAPLFVPGLGA
                     FIRLRQELTDPKQDARVGLAGPFWGLGAALVCLAAYVVTRQPILAALAQFGAIINLFN
                     LLPIWHLDGGRAFRSLTRSQRWLAVAAIAAAWAITDEGLLVLLLLGGIVRTLMDKPSD
                     KPDPSVVGQYAILIWILAALTRLPVPLPG"
     gene            complement(2899..3834)
                     /locus_tag="VHS97_24750"
     CDS             complement(2899..3834)
                     /locus_tag="VHS97_24750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451485.1"
                     /translation="MKTKLEMMVAYLAGRKGDAPESIRRELEDPTSEASRCLEAVRWR
                     SRGVFGAELPEMADPVPSRSIRNRASARATPVRRLPSLLVGATSAAVVFIAVGMAWRA
                     QDNRLLHLETTLARREDRLGDRFNRLEATLTRRETPSQKQPASSTGPNLQEPKPPTTA
                     DRPTILALARIEARLGELEQQLGERQPRQDQDDQQVAQLRRDLDRLRQEAEIAVRASK
                     QESQRLSMAVGEILTLLRLLTMQSRGTEPMQVPVPIPVPPLGQGPGVGQGPGMMPGPG
                     QMPGQAQMPGHAQMPGQAQMPGQDFPNMAPGRGKR"
     gene            complement(3831..4544)
                     /locus_tag="VHS97_24755"
     CDS             complement(3831..4544)
                     /locus_tag="VHS97_24755"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3451486.1"
                     /translation="MGTFAQKNGRPTAAAAEERPTCDAAYERVAREFLRQLALDHDEA
                     FAAAVALRFKEAPAKICCRAIGGWIDQKLLGFGWTQHDLADRIGVDRSAVAKWTAGGA
                     ISLGHLVLVLLEFRGDFADLPLPARRELALEGYLAALSHVRARIDPEAAREPLDRERF
                     WCLFHLFSEPHWERAIRRQDRELVKQEADRVLRRAGESLGTRPRRVVGVEGLRRLVEE
                     WTAAWVICLELLPGSWSIR"
     gene            5265..5666
                     /gene="rpsL"
                     /locus_tag="VHS97_24760"
     CDS             5265..5666
                     /gene="rpsL"
                     /locus_tag="VHS97_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX3451487.1"
                     /translation="MPTINQLVRKPRRPVKYKTKAPVLEASPFKRGVCLQVKTMTPKK
                     PNSALRKVARVRLSNGKEITAYIGGEGHNLQEHSIVLVRGGRVRDLPGVRYHIVRGVL
                     DCQGVAERKQARSKYGAQRKKAAAPGAAKKK"
     gene            5741..6214
                     /gene="rpsG"
                     /locus_tag="VHS97_24765"
     CDS             5741..6214
                     /gene="rpsG"
                     /locus_tag="VHS97_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX3451488.1"
                     /translation="MARKFTASKTHLRPDPRFGSKLAGKFINCVMHNGKKSVAQRLFY
                     DAMDLIQKRLPNENPIDVFNRAVENVKPAIEVRSKRVGGATYQVPMQVNKNRQQTLSI
                     RWLLMAAREKKGRPMAIKLADELMAAYNREGAAVTRRENVHRMADANKAFAHFAW"
     gene            6267..7544
                     /locus_tag="VHS97_24770"
     CDS             6267..7544
                     /locus_tag="VHS97_24770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451489.1"
                     /translation="MGQDPLHVLWVEPNFPGRLGAVADWLVRRRGYRCSFYCHTADPR
                     STWPPSVGKGLDVQTFGVGGVARDASVNWSRVLERSLCYAYGCWEVLEKRRPRPIDLI
                     VGRSNGLGSTLFAPVYWPAAPVANFVDYYYHAHDHDLAGEAGPETSPAYFHWRRSMAA
                     IDLLDLEQTALAWTPTHWQRDLFPSEYREQFLVLHDGIDTERFARSAWHAAAAGPRSI
                     AGRVVADDVRIVTFVARSLDRLRGFDRFLTIANELLRTRDDVICVVVGDPIVRRGLDV
                     EFHNRDYAAHLMAKQPPFDPDRVWFLGPSTPTLVAEVLAAADLHIAPSRPYPVARSLL
                     EAMAAGCIVLASDTAPHREIVEPGRSGLLVSGQDTDALKSQALAVLADIALYRSLGDA
                     ASVLVHEHYSQDVCIPRLAERFTALAVAGGQPS"
     gene            7541..>8278
                     /locus_tag="VHS97_24775"
     CDS             7541..>8278
                     /locus_tag="VHS97_24775"
                     /inference="COORDINATES: protein motif:HMM:NF023426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX3451490.1"
                     /translation="MNILFIHDAFPAQFGRLGLELTRRNGWRCSFLVQSLSSCPTASS
                     EMLETLELHKVPLAPEHRNSDGIPWPQIYGQYLEHCRVVHDAIKAKPNLRPDLIVAHG
                     GRGAPALLLREVVDCPIINYCEYYFANSHRDISYRIDLPPAEPAPFFPRCINARTLAT
                     LVDCDAGYSPTQWQKESFPRRFHTKIEVHFDGIETDLYHPGPARRQLGETVIAAGTRI
                     ITFVSRGLESIRGFDLFMKVAHRIAAAR"
BASE COUNT         1481 a         2631 c         2531 g         1635 t
ORIGIN      
        1 gaggaggcca gggaggcctt tcgcagccgg gcatatcatg cccacccgga tcgggggggc
       61 gaaacaaaga ggtttatccg gatccgcaag gcctatgaac aaatcctcgc cgatctggag
      121 cgccagcctg tccccgatgc cgataagaat gagcaagccg tggacaagag cgcccaatcg
      181 ccacaccgcg accgccgctc gaagccgccc gatcccaact gggagcccga ctttgtcttg
      241 cttgacgaac cgccgcgccg tactcgtccc cctcggccgc gcgatcccaa ctggaagccc
      301 gacctgatcc tgctcgacga cccggcttcc gacagggaaa gtacaaattc cgccgatgtc
      361 tcgtggaaaa cctcccgcgc ccttttccaa cgcatttcag accagtcaca gcaaaaggat
      421 tcgacgaggc aatttgggtg gattcatgcc gtcgggatgt tgattttatt catgatgatc
      481 gtcctcctta gcctcacgct cttttggacg ttttcaacct ccgagcggca aaccgagcca
      541 gtcgagagtc caacatacta tcccgcggta caatgatgtg ccggctgcag ctttgagctc
      601 gtgtcgatca cagttgactt caacaagtct ttcgccggcc gttctttgtt ttgtggcgaa
      661 gtgttaccta tagtggagtg atccggccgt taatccggtc gcatgctagc ctcttttctg
      721 ccaaccaagg aatgatcatg ccggagcaaa atgactccgt cgaccgaggt gacgacgacg
      781 ttgccatcaa cccggtcgac atccagttcg accacgcgga gtacgcgact cctgcccccg
      841 atggtccgag ttgcggtgtc tgccagagtc cgatcgaagg cgaatattat gagacgggcg
      901 gcaaggtttg ctgcccgaca tgccgccaca atattgaggc ggcccttcgc ggcggatcgc
      961 gcgttgccag ggtgctcaag tcgctaatcc ttggttcggc ggcatcggcg gtcggtgcgg
     1021 tgctttatta cgcgatcctg cgcgggaccg gctacaacat cggcctggtt gcggtcgttg
     1081 tcggcgtcat tgtcggcagg gcggtgcgca agggtacggg cggccgcggc ggccgatttt
     1141 atcagttcct cgccgtcttt ctgacctata ctgcgatcgt ggtaatgtac gttccgctgg
     1201 tgttagaagg attgagcaaa ccagctcaga atgcaccgca agccatgcct caggtcgcga
     1261 aagcgggctc ggactccgcg aaatccgaag cccgatccaa agttgccgac gccagcaagg
     1321 atggtccatc gggtcccaag gcggacagtc cgaccgatcc ggccgccgcg cccgagatta
     1381 ctgcgaagga tagggcggtg acgccccagg aaaagaccgc cgtcgttgat gacggaggtg
     1441 ccgccgatgt gaaggaggtg ccgccagtac ataagccggc gcagatcccg ctggctttac
     1501 tggtagccac ggtattctta atcggccttt tctacacggt tccggtgcag atagccgtgg
     1561 ccgctcccat ctccggactg atattcgcat ttgcgctctg ggaggcatgg aaggctaaca
     1621 ggggagtgca cctgtcgttc aacggacctt ttcgcgtgag cgcgaggggc tcgagcggac
     1681 cggagccaga ggtcgagcgt gatggccgat gagttgagat cgcgcgtccc gctgttggcg
     1741 cataactgta cggtttgtgg cagcgagatc gcgcccacgc tcctgagctg cccatcctgc
     1801 caccgcttgg tacatggcgc acggctcaaa gagctggcgg ccgacgcgga ggccgccgaa
     1861 aaggccggcg agttgtcggc cgccttggct ttctggaacg aagccgtgtc gctgttgccg
     1921 gcggaaagtc gacagtacgc ggccatcgcc gatcgaatcg ccatgctggg caggcgcgta
     1981 gaggccgggc cgaaaccgcc tgcgcagtcc acgcccaccg taccgcatgc caaaccaaca
     2041 gcgtcgtctt cttcaccgtg gtccggcggc gcactctcgg gaatcgccgc aaccctggcc
     2101 ctccttgcct ggaagtttaa gtttctggcg gtgctcttac tgaccaaggg caaaattctc
     2161 ttgctggggc tgaccaaggc gagcacgttt ctgtcgatgt ttgtttcgat gggcatgtac
     2221 tggaccgtct tcggcgggtg gttcgccgcg ggtctggtat tgtcgatcta cgtccacgaa
     2281 atgggccacg tcgtggcact gtcgcgctat gggattcacg ccggagctcc tctattcgtg
     2341 ccgggcctgg gtgcgttcat tcgcttgagg caagagctga ccgacccgaa acaggacgcc
     2401 cgagtcgggt tggccgggcc gttctgggga ctgggcgccg cactcgtgtg cctggcagcg
     2461 tacgttgtca cccggcagcc gatcctggct gccctggccc agttcggggc catcatcaac
     2521 ctgttcaatc ttctgccgat ctggcacctt gacggcggac gggcctttcg cagcctgacc
     2581 cgctcgcaac gttggcttgc cgtcgccgcg atcgccgccg cttgggccat cacagacgag
     2641 gggcttttgg tcttgttact tctgggcggg attgtccgta cgctcatgga caaacccagc
     2701 gacaaacccg acccgtctgt ggtcggtcag tacgcgatct tgatctggat tctcgcggcc
     2761 ttgacgcgct tgcccgtgcc gctgccgggt tagatgtcgg cgatctcgcg gttggccttc
     2821 tcgtaggtca aattcgagat cgggaagaaa cgggcaggga ctgcatgcca gtccaattaa
     2881 ggctcctcga tatccccgtt accgtttacc ccggccgggg gccatattgg gaaaatcctg
     2941 gcctggcatt tgagcttgac ctggcatttg tgcatgacct ggcatttgag cttgacctgg
     3001 catttgcccc ggcccgggca tcataccggg tccttggcca actcccggcc cttgccctag
     3061 tggcgggacc gggatcggca ctggaacttg catcggctcc gtcccccgcg attgcattgt
     3121 caggagtcgg aggagtgtga ggatctctcc gacggccatg ctcaacctct ggctctcctg
     3181 cttgctggcc cggacggcaa tctcggcctc ttgcctgaga cgatcgaggt cccgccgaag
     3241 ttgggcgacc tgctggtcgt cttggtcttg cctcggctgt ctttcgccga gttgttgttc
     3301 gagctcgccg agcctggcct cgatccgagc tagcgccaaa atcgtcggcc tgtctgctgt
     3361 tgtcggcggc tttggctctt ggaggtttgg tccagtcgag ctagcgggct gtttttgcga
     3421 aggtgtctcc cgtctcgtga gggtcgcctc gagacggttg aaacggtctc ccaaccgatc
     3481 ctcgcgccga gcgagtgtcg tttcgaggtg gaggagccgg ttatcctgcg cccgccacgc
     3541 catacccacc gcgatgaaca ccaccgcagc agacgtggcg ccgacgagca gcgaaggcag
     3601 ccgtctcacg ggcgtggcgc gtgcgctcgc ccgattgcga attgagcgag acgggacggg
     3661 atcggccatc tccggcagtt ccgcgccgaa gaccccccgc gatcgccacc gcactgcctc
     3721 caagcagcgg ctcgcctcgc tcgtcgggtc ctcgagctcg cgccggatcg attccggggc
     3781 gtcccccttc cgcccggcca ggtacgcgac catcatctcc agcttggtct tcatcggatc
     3841 gaccagctcc ccggtaagag ttcgaggcag attacccacg cggccgtcca ctcctcgaca
     3901 aggcgcctca ggccctcgac acccacaacc cgccggggcc gggtgccgag cgactcgccc
     3961 gcccgcctga ggacccggtc cgcctcctgc ttgacgagct cgcggtcctg ccgccggatc
     4021 gcccgttccc agtgcggctc ggaaaacaga tggaagaggc accagaaacg ctcgcggtcg
     4081 aggggctcac gagccgcctc gggatcgatc cttgcccgga cgtgcgatag ggccgcgagg
     4141 taaccctcca gggcgagctc gcggcgggcc ggcagcggca gatcggcgaa gtcgccgcgg
     4201 aactcgagca ggaccaaaac gagatgcccc agcgagatgg ccccgcccgc ggtccacttg
     4261 gccacggcgg agcggtcgac gccgatccgg tcggccaggt catgttgggt ccagccgaag
     4321 ccgaggagct tctgatcgat ccatccgccg atcgctcggc agcagatctt cgccggtgcc
     4381 tccttgaagc ggagcgcgac cgcagcggcg aatgcttcat catggtcgag cgcaagctgg
     4441 cggaggaact cccgagcgac cctttcatag gccgcgtcgc aggtgggccg ttcctccgcg
     4501 gcggccgccg tcgggcggcc atttttctgg gcgaatgtcc ccatcgccgc gtccctcctc
     4561 gtgcggggcg cgacttcgcc cctcctgagg gactccatca taccggaacg gctcggcgga
     4621 aagtgagagg cattcacgtt cctgcccctg gcggtcgcat acgatgcttt aaggacgtga
     4681 gcccattgcg gagccacgcc gatctgatga tcacggtcga gaagagcgac gagagaaaaa
     4741 ggggcaggcc gcagggacgc tggctgcccc aaccccatcc ccatcggcgc tcggtgagcc
     4801 accgccgagc cgatggggct cagtttcgaa tcgtgcatcc ccttttggat cgccccaaat
     4861 cgaaggtttc actcctgtga ttctccgcat ttcccaaatc tgtcggcgca ctccgtgagg
     4921 cgattcttct cgacgaactt cttgacaatc ctcccgctta ggttatccta aaagcttcgg
     4981 caattggtga atccaagccc actgcgcgcc gtgccggatg gtgcgtgcgg ccgggtcgga
     5041 ccgtcaggcc gtgcgtcgag agcaaagcga ttgccattgg ttcgggtcga tcgagagccg
     5101 cgcgacgcgg cccgaaacga ccggcccaga agcgtcgtca acatcgagtc agtgacatcc
     5161 cctcggcgtg gaacgccggt tcgaggcgat cgcgagcttt cgcgattttg ccggatcgaa
     5221 tcgcacgtct cgtcaagccg tcgggacaat ggaacgacga agtaatgccc acgataaacc
     5281 agcttgtccg taagccgcgc cgaccggtga aatacaagac caaggcgcct gtgctggaag
     5341 catctccttt caagcggggt gtctgcctac aggtcaagac gatgacgccc aaaaagccca
     5401 actcggccct acgtaaagtg gcccgcgtac ggctttccaa cggtaaggaa atcacggcct
     5461 acatcggtgg cgaagggcac aacctgcagg aacactccat cgtcttggtt cgtggcggcc
     5521 gcgtccgaga cttgccgggc gtgcgttatc acatcgtgcg gggtgtactt gattgccagg
     5581 gagtcgccga acgaaagcag gcgcgctcca agtacggagc ccagcgcaag aaggccgccg
     5641 caccgggcgc cgccaagaag aagtgagtcg gcaacccccc agtctggaac tgattgctga
     5701 agacatgttt gtgatcgttt aacgagcagg aacgacccag atggcccgaa agttcacagc
     5761 cagcaagacc caccttcgcc ccgatcctcg tttcggctct aagctggccg gaaaatttat
     5821 caattgtgtg atgcacaacg gcaagaagag cgtggcacaa cggctcttct atgacgcaat
     5881 ggacctgatt caaaagcggc tgcccaacga aaacccgatc gacgtcttca atcgcgccgt
     5941 tgaaaacgtc aagccggcga tcgaagttcg ctctaagcga gtcggcgggg caacttacca
     6001 ggttcctatg caagtcaaca aaaatcgtca gcaaaccctt tccatccgct ggctcttgat
     6061 ggcggcgagg gagaagaaag ggcggcccat ggcgatcaag ctggcggacg agctgatggc
     6121 ggcttataac cgcgaaggtg ccgcagtgac gcgccgcgaa aacgtgcacc gcatggccga
     6181 cgccaacaag gcgtttgccc atttcgcgtg gtgagacgga tcggcttcgg cctgactttt
     6241 tcacgcgttt agacccgcgc tattccatgg gtcaagaccc gctccacgtg ctctgggtag
     6301 agcccaactt tcccggccgg ctgggagcgg tagccgattg gctagtgcgg cgccgcggtt
     6361 accgttgttc gttttactgc cacacggctg atccacgttc gacctggccg ccatcggtgg
     6421 gcaaagggtt ggatgtccag acgttcgggg tcgggggcgt ggcgcgcgat gcgtctgtca
     6481 actggtcgcg cgttctggag cgtagcctct gctatgccta cggctgctgg gaagttctcg
     6541 aaaagcggcg gccccggccg atcgacctga tcgtgggtcg ttccaatggg ctgggatcca
     6601 ctcttttcgc cccggtctat tggccggcag ctccggttgc taacttcgtc gactattact
     6661 atcacgccca cgaccacgat ctggctggtg aagccggccc cgaaacctcg cccgcctatt
     6721 ttcactggcg gcggtcgatg gcggcgatcg atctcttgga ccttgagcaa actgcgctcg
     6781 cctggacgcc gactcactgg caacgcgact tgttcccgag tgaataccgc gagcaattcc
     6841 tggttttgca cgacggcata gacacagagc gcttcgcccg gtcggcatgg catgccgccg
     6901 ccgccggccc cagatcgatt gcaggccgag tcgtcgcgga cgatgtgcgg atcgtcacgt
     6961 tcgtggcccg gtcgctcgac cggctgcgag ggttcgaccg cttcctgact atcgccaacg
     7021 aactgctgcg gacacgcgac gacgtcattt gcgtcgtcgt gggcgacccg atcgtccgtc
     7081 gaggtctgga cgtcgaattt cataatcgcg attatgccgc tcacctgatg gcaaagcagc
     7141 ccccgttcga tcccgatcgg gtctggtttc ttgggccttc gacacctacg ctagtggcgg
     7201 aggtcctggc cgccgctgac ttgcacattg ctcccagccg gccctatccg gtcgctcgct
     7261 ccctcctgga ggcgatggcc gcgggatgca ttgtgttggc ctccgatacg gctccgcacc
     7321 gtgagatcgt tgagccgggg cgatccggcc tgctggtgag cggtcaggat accgatgccc
     7381 ttaaaagtca ggccctggcg gtgctcgccg acatcgccct ataccggtcg ctgggcgacg
     7441 ccgcctcggt gctggtgcac gagcactact ctcaggatgt gtgcatacct cgactcgccg
     7501 agcggttcac ggctcttgcc gtcgctgggg gtcaaccgtc gtgaacatct tgttcatcca
     7561 cgacgcgttc cccgcccagt tcggccggct cggtctggaa ctgacccggc gcaacggttg
     7621 gcggtgcagc tttttggtgc agagcctttc gagctgtccg accgcatcgt ccgagatgct
     7681 tgagacgctc gagcttcaca aagtgcctct cgccccggag cacagaaata gtgacggcat
     7741 tccctggccc cagatctacg gacagtacct cgaacactgt cgggtcgtcc acgacgcgat
     7801 caaagccaag ccgaatctac gacccgacct gatcgtcgcg catggcggcc gcggcgcgcc
     7861 cgcgctcttg ctccgtgaag tcgtcgactg cccgatcatc aactactgtg aatattactt
     7921 tgccaatagc catcgcgata tctcgtatcg gatcgacttg ccgccggccg agcctgcgcc
     7981 gttctttccc cgctgcatca atgcacggac gctggcaacg ctggtcgact gcgatgcggg
     8041 ctactcgccg acccagtggc agaaagaatc gttcccacgc cgctttcaca ccaaaatcga
     8101 ggtgcatttc gatgggatcg agaccgatct ctatcacccg gggccggcgc ggcggcaatt
     8161 gggtgaaacg gtcattgctg ccggcacccg tattatcacg ttcgtctccc gcggcctcga
     8221 atcgatccgg ggcttcgatc tattcatgaa ggtggcccac cggatcgccg cggcacgc
//
LOCUS       DATAQL010001434         4327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2285698, whole genome shotgun sequence.
ACCESSION   DATAQL010001434 DATAQL010000000
VERSION     DATAQL010001434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285698"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2306
                     /locus_tag="VHS97_24780"
     CDS             <1..2306
                     /locus_tag="VHS97_24780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451491.1"
                     /translation="LFHQTLFCSKVLSPADVLLVSAGFRGDGAGDYEPANRLLMDPVL
                     QFQPWLEFSRRMIRSGKLPLWNGHAGCGAPHLANGQSAVFDPFHLLAYLGPTPEAYAW
                     IAAARLWVAGLGMFLLGRAWGLGPWGRWFAGLVYPFCGFLVLWLLFPVTAVAIWMPWL
                     FLATDRVFRAPGLKGAGGLAVVVALIIFGGHIQTSAHVLLAGGLYALAKGYWERADPS
                     KARQAVISWAVGTCLGLGLAAVQIIPLGFYLAKSSVWSDRQSERPPWWTIDRPRLLDA
                     ACTAVPYLYGSQRRGHPNVARALGVHNLNESAGGYTGLATLIWLAPLAVITRGRMPQV
                     AFLTGLVVVGSMGAFRLPPVDNLLRSLPVLDVTDNRRLTLWVAFGLTLLGGIGLDQLH
                     QSRRIARSWLIAWIVGGLLLGSVACSIPMFERPLRERANAHYRQAASSSPGADTQTYQ
                     TRADRQVRQIVGFFPAYHGLIAAELGLLTALATCVRRRRGCPFWIQPVVLGLTLFDLA
                     LLGFDLNPAISASVHSFEPALIARLRQGVPKNGRVLGLGEELPPNTLMRFGLDDPRNY
                     DSVELASSLAWFAPLFEPTGSSRSSRGEITWSGVIAARDRLRESGVSAVVAALSPPEM
                     TFDKVERVGRVWIAWQHAKPWASCVSPRSRLDVVRDHGWARILVDLEEADRLTIRETW
                     DSGWTALLDGVPVRIRPKSRVFLDVDIPSGQHKLILKYDPIEVRIGLAFSLGGLVLLI
                     LVLTGIPLFWIPGVSTAKGLDGAEPSG"
     gene            2368..2676
                     /locus_tag="VHS97_24785"
     CDS             2368..2676
                     /locus_tag="VHS97_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451492.1"
                     /translation="MVHFTCDLCGKELTGSGESRYVVKIEAYPGFDPTEIKEDDLDDD
                     PMEAVAQILQRDEALVSEDLETSLPKAFRFDLCGSCHVKFVKDPLGKDAIRTFDFSKN
                     "
     gene            complement(2691..3986)
                     /locus_tag="VHS97_24790"
     CDS             complement(2691..3986)
                     /locus_tag="VHS97_24790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3451493.1"
                     /translation="MDSRVESGGPGKDPQSSSTVDETQLEKSIIRRGLATPAEIEACK
                     AQRQQLVAKENDSSQSLLEIMVEAKVLTRSQVTRLVQQVGDANRKFQIPGYQMIQKLG
                     KGSMGIVFKAKQISVDRVVAIKILLDPLAQNKEFIKRFEREAMIAAKLSHNNVVNAID
                     AGQIDGHYYFVMEYVEGPTIKDYLDKNKTFDEKEALRITLAIAEALKHAAQRGLIHRD
                     IKPENVILTRQGEVKLADLGLARLTGDEKWGLSEAGMAIGTPYYISPEQVRGQTNIDI
                     RADIYSLGATLYHMVTGRVPYGGETPNEVMRKHVDPNVAMVPPDHLNSRLSGGLGMVV
                     ETMMAKNREHRYQNPDDLILDIKCLQRGESPMIAGQKPETLEALAEGEFDEQYRRGAS
                     EDELVELASYVNNRNNVIATMAMLLAVSVITNVILFVAR"
BASE COUNT          735 a         1280 c         1311 g         1001 t
ORIGIN      
        1 ccctttttca tcaaacgctc ttctgcagca aggtcttgag cccggccgat gtcttgctgg
       61 tttcggccgg ttttcgcggc gacggggcag gggattacga gccggcgaac cggcttttga
      121 tggacccagt cctccagttt cagccttggc tcgaattcag ccgacggatg atcagaagcg
      181 gcaagctgcc gctttggaac ggccatgccg gctgtggcgc gccccatctc gcgaatggtc
      241 agagcgcggt ttttgacccc ttccatcttc tggcctacct tggtccgacc ccagaagcgt
      301 atgcctggat cgctgcggcc cggctgtggg tggccgggct gggaatgttc ctgctgggac
      361 gcgcttgggg gctcggaccc tgggggcggt ggttcgcggg attggtctac ccgttttgcg
      421 gctttctcgt gctatggctg ctgtttccgg tcaccgccgt ggcgatctgg atgccctggc
      481 tgttcctggc gaccgatcgc gtctttcgag cgcccgggct caaaggggcc ggcgggctcg
      541 ccgtcgtggt cgcactgatc atcttcgggg gacacatcca gacatccgct cacgtgctgc
      601 tggccggagg actctatgca ctcgccaagg gatactggga acgcgccgac ccgtccaagg
      661 cacggcaagc cgtcatttcc tgggcggtgg ggacgtgttt ggggttggga ctggcggcgg
      721 ttcagatcat tccgctgggg ttttatctcg ccaagagctc ggtctggagc gaccgtcaga
      781 gcgagcggcc cccgtggtgg acgatcgatc gtcctcgact actcgacgcc gcgtgcacgg
      841 cagtacctta tctctatggg agccagcgcc ggggacatcc caatgtggct cgagccctgg
      901 gcgtccacaa cctgaatgag tcggccggcg gctatacggg cctcgcgacc ttgatctggc
      961 tggcgcccct ggcggtgatc acgcgcgggc gcatgcccca ggttgctttc ctgaccggtc
     1021 tcgtggttgt cggctccatg ggggcgtttc gcttgccacc ggtcgacaac ctcctgcgca
     1081 gcctgcccgt tctcgatgtc accgacaatc gcagactcac gctttgggtc gccttcggtt
     1141 tgacgcttct gggtggaatc ggtctcgatc aactgcacca atcgcggcgg atcgcgcgaa
     1201 gctggctcat tgcatggatc gtcggagggc tattgctggg ttcagttgcc tgctcgatcc
     1261 caatgttcga acgcccgctt cgggaacggg caaatgccca ctaccgacag gccgcatcgt
     1321 cgagcccggg cgccgacacc cagacctacc agacacgggc cgatcgccag gtgcggcaga
     1381 tcgtcggctt tttcccggct taccatggcc tcatagcagc tgaactcggc ctgctgaccg
     1441 cgctcgcgac atgtgtacgt cgtcgtcgtg gctgtccgtt ctggattcag cccgtagtgc
     1501 tcggccttac attgttcgac ctggcattgc taggtttcga tctcaatccc gcaatttccg
     1561 ccagcgtcca ttctttcgag ccggcactga tcgcacggct gcggcaaggt gtgcccaaga
     1621 atggccgcgt tcttgggttg ggcgaagaac taccccccaa caccttgatg cggttcggcc
     1681 ttgacgatcc gcgcaactac gattcggtcg agctggcctc cagcctggcc tggtttgcac
     1741 cgctctttga accgaccggg tcctcgcggt cgagccgcgg cgagatcaca tggagcggag
     1801 tgatcgccgc gcgcgatcgg ctgcgcgagt cgggagtcag cgcggtcgta gccgccctta
     1861 gcccgcccga gatgacattc gacaaggtag agagggtcgg ccgtgtctgg atcgcctggc
     1921 agcacgccaa gccctgggct agttgcgtgt cgcctcggag ccggctcgac gtggttcggg
     1981 accacggctg ggcccgtatt ctggtcgatt tagaagaagc cgatcggctc acgatccgcg
     2041 aaacgtggga ctctggttgg acggccctgc tcgacggcgt gcccgtcaga attcggccaa
     2101 aatcgagggt tttcttggat gttgatatac cgtcgggtca gcataagttg atcttaaagt
     2161 acgatccgat cgaggttcgg atcggcttgg cattctcact tggcggtttg gttctcttga
     2221 ttctggtgtt gacaggaatt ccattgtttt ggattcctgg agtaagcact gcaaagggct
     2281 tggacggagc tgagccctcg ggttagaatc gtggtatcac cttccggact cgttaaccgg
     2341 ctcataaaac tgagggatgt aacgctgatg gtccacttca cgtgcgatct ctgcggcaag
     2401 gaattgaccg gttccggcga aagccgttac gtggtcaaga tagaagccta tcccggcttc
     2461 gacccgacgg aaatcaagga ggacgatctg gatgatgatc cgatggaggc tgttgcccag
     2521 atcttacagc gcgatgaggc cctcgtgagc gaagaccttg agacgtcact gcccaaggca
     2581 ttccgattcg atctttgtgg gtcttgtcac gttaagtttg tcaaagaccc tctgggaaaa
     2641 gacgcgatcc gtactttcga cttcagcaag aactgaagcg cacggtctct ctaacgcgca
     2701 acgaagagga tcacgttggt gatcaccgaa acggccagga gcatcgccat cgtggcgatc
     2761 acgttgttgc ggttattgac gtaggaggcg agttcgacga gctcgtcttc gctagcgccg
     2821 cgacgatact gctcgtcaaa ctccccttcg gccagtgcct cgagtgtttc gggcttctga
     2881 ccggcgatca tcgggctttc gccccgctgc aggcacttga tatcgaggat cagatcatcg
     2941 gggttttggt agcggtgctc gcggttcttg gccatcattg tttccacaac cattcccagg
     3001 ccgccggaaa gcctggaatt gagatggtcg gggggcacca tggccacgtt gggatcgacg
     3061 tgcttgcgca tcacttcatt gggtgtttca ccgccgtagg gaactctgcc tgtgaccatg
     3121 tggtacagcg ttgcccccag gctgtagatg tcggcgcgga tgtcgatatt ggtctgaccg
     3181 cgcacctgct cggggctgat atagtacggc gtgccaatcg ccatgccggc ttccgaaagc
     3241 ccccatttct cgtcgccggt cagtcgagct aaaccgagat cagccagctt gacttccccc
     3301 tgccgcgtca gaatcacgtt ttcaggcttg atgtcgcggt ggatcaaacc gcgctgggcg
     3361 gcgtgtttca aggcttcggc gatcgccagc gtgatacgca gtgcctcttt ctcgtcgaac
     3421 gtcttgttct tgtcgaggta atccttgatg gtcggtcctt ccacgtactc catcacgaaa
     3481 tagtaatggc cgtcgatctg gccggcgtcg atggcgttga cgacattgtt atgcgatagc
     3541 ttggcggcga tcatcgcctc tcgctcgaag cgcttgatga actctttgtt ctgcgcgagg
     3601 ggatcgagca gaatcttgat cgccaccacc cggtcgacgc tgatctgctt ggccttgaag
     3661 acaatcccca tcgagccttt gccgagcttc tggatcatct gatagcccgg gatctgaaac
     3721 ttgcgattgg cgtcgccgac ttgttgcacc agccgcgtta cctggctgcg ggtcaagacc
     3781 ttggcctcga ccatgatttc cagcaggctt tgggaagaat cgttttcctt ggcgaccagt
     3841 tgctgacgct gcgccttgca agcctcgatc tcggcgggcg tggccagccc gcgacgaatg
     3901 atcgatttct ccaactgagt ctcgtcgacc gtcgacgaac tctgggggtc tttgccaggc
     3961 ccgccacttt cgacccgact gtccatctcg cagtctcctt ctctgcctaa ctcaccggac
     4021 tcgaccgatc gattacaatc cggccctggt cctggcggca gaccatgctc taggcctgcg
     4081 tcgcgaggtc aagacaatgc gcgtctctcg cacgggattc ggttcatgaa actatgtttg
     4141 tcaacacccg cttgtcaacg tggttctcag cccgattgcg ctatcaagcg ctcgggctgt
     4201 cagaacgcga cctggcgagt atgactgatg agtattaccg acacttgagc ggagaggtgc
     4261 cgtcttttat attacatgcg ctcggcacgc tattctacga tcttcaatcg cgatggtgac
     4321 gatcgga
//
LOCUS       DATAQL010001435         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_148891, whole genome shotgun sequence.
ACCESSION   DATAQL010001435 DATAQL010000000
VERSION     DATAQL010001435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148891"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VHS97_24795"
     CDS             <1..643
                     /locus_tag="VHS97_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451494.1"
                     /translation="GSIFLNAAENDEKLELSKIVPSKFGDADTKITTTLELADIVRKT
                     ASLGATYPQIVSILENAKRQRNLAGELVVDAVPVSNRVYLEAVLGKDTTAKRDDSVKR
                     ASGESSRSGRRWLWGIFGRNTDDAATKKSSSDKSSNNASSDDADSSKSNTLGDPSAKN
                     NETDSKRPEKAKQGDAGAGDSSAKKDDAVQKAKVDDQAPARPRLFDLFRRDDE"
     gene            1410..1985
                     /locus_tag="VHS97_24800"
     CDS             1410..1985
                     /locus_tag="VHS97_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3451495.1"
                     /translation="MKKVFTTGQVAKICKVAPRTVSKWFDSGRLRGYRIPGSQDRRIP
                     REHLLRFLKEHGMPLGDLEAEVYHKVLVVGADAPLQSILRDHLRETDDFRIETAASGF
                     EAGIRAESFHPDCIIIDMALGRIEAGQIAQNLRKSPDHLGTILLALTSDEPGEETFAL
                     GFNDAFKKPFDGALLAERVRRLISQKKADEP"
BASE COUNT          465 a          647 c          618 g          401 t
ORIGIN      
        1 aggctcgatc ttcttgaatg ccgccgagaa cgacgaaaag ctcgagctga gcaagatcgt
       61 tcccagcaaa ttcggcgatg ccgacaccaa gatcacgacc acgctcgagc tcgcagatat
      121 cgtgcgcaag acggccagcc tgggggcaac atatccccag atcgtcagca tcctcgagaa
      181 cgcgaagcgt caaaggaacc tggctggcga actggttgtc gacgccgtgc cggtttccaa
      241 ccgcgtctac ctggaggccg tgctcggcaa ggacacgact gccaagcgcg acgactctgt
      301 gaagcgagcc tcgggtgaat cctccaggtc aggccgacgg tggctgtggg gaatcttcgg
      361 tagaaacacc gacgacgccg ccaccaagaa atcctcctcc gataaatcaa gcaacaatgc
      421 atcctctgat gacgctgatt cgtccaagag caacactctc ggcgatccgt ccgccaaaaa
      481 taatgagacc gactcgaaac gtcctgagaa ggctaaacaa ggcgacgcgg gggccggtga
      541 ctcatcagcc aaaaaagatg acgccgttca aaaggccaag gtggatgacc aggcaccggc
      601 ccggccgcgg ctgtttgatc tctttcgtcg cgacgatgag tgatggacca ggccagcgag
      661 gtctgattgg tcgcattctt gaagtaaaac taaaatccat ttcaagttcg gtaaactgca
      721 accatcgcct aggccccaga aacaggaatg acggcgtccc ggttgcgtca gccattgccg
      781 gtcatcccaa tgagctcaag caatcacttc cccccgctgg cggtgcccag tgcccggacc
      841 gacgagacaa cattttgtct aattttgcat ctccgacgta actcctacgc gcagcacgga
      901 cttcacaagc ctgttagccg ccaagatcgg cttgcatggt ggggctagtc ctcgcccctc
      961 ttaaagtccc acgccgcgcc gttcgaatat tagtcttgac ggtcaggtcc actacagcac
     1021 ggaggcaagc gacacctctt tgttggccac gggccagacc gctccccgcg cacgacgcgg
     1081 cgggggggtc tccgtacttg gtcggagtcg agtcgggttc ccgatccgtc actcaactca
     1141 cggaaacggc agtggaaatc ttgagagaaa gagcagttat gccgctcttt tgatcgggct
     1201 ctacgctgct gcctagacag gaccaggcgc gggggcgggg tcggagcgac ttgcacgatt
     1261 gccgtctctc gcggcgattc aagcgcattc gcatgcgttt gagcccaacc gccgagccgg
     1321 gactgtcacc gcgccgacat ggttcctatc cggagggcgg aggaaacctc cgtggtctcg
     1381 caccaatctg ggcgagtgga gtcctcgtga tgaaaaaggt gtttacaacc ggccaggtcg
     1441 cgaaaatctg caaagtcgcg cctcggaccg taagcaagtg gttcgactcg ggtcgcttgc
     1501 gtggttatcg catccccggc tcgcaggatc gtcgcatccc gcgggagcat ctcctgcggt
     1561 tcctcaagga gcatggaatg cccttgggcg acctcgaagc cgaggtttat cataaggtgt
     1621 tggttgttgg agccgacgct ccgctgcagt cgatcttgcg cgatcatctt cgcgaaaccg
     1681 atgatttccg tatcgagacg gctgcttcgg gctttgaagc ggggattcgt gccgagagtt
     1741 ttcatcccga ttgcatcatc atcgacatgg ccctaggacg catcgaagcc ggtcagatcg
     1801 cgcagaatct tcgcaaaagc ccagaccacc tgggcacgat tctgctcgct ttgaccagcg
     1861 acgaacctgg cgaggagacc ttcgcgctcg ggttcaacga cgcgttcaag aagccgttcg
     1921 acggagcgct cctggccgag cgtgtccgcc gcctgatctc gcagaagaag gccgacgaac
     1981 cctgaggcac aacgccaacg ggaacccgac ttctacctcg gcagccaacc gaggcgaacg
     2041 agcccaacgg tctgctctca cccggtcaaa gccggtgaga ggggccgtcg ggctcgtttg
     2101 ttattttgac acagccgtac gattgcgcgc g
//
LOCUS       DATAQL010001436         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3050495, whole genome shotgun sequence.
ACCESSION   DATAQL010001436 DATAQL010000000
VERSION     DATAQL010001436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050495"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..1723
                     /locus_tag="VHS97_24805"
     CDS             404..1723
                     /locus_tag="VHS97_24805"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX3451496.1"
                     /translation="MASSTDTALQPTLPARPIERVAILFAGGPAPAANAVISTAAASF
                     RKIGIEVLGIMHGYAHLIDYRDDKPLQEGRDYIVLDQNNLKRTRNTRGILVGTSRTNP
                     GKDISQPAHLADPERTAQLRAVHRALKSLGVNALVSIGGDDTLKTANKFKRFQDYLPP
                     EEPRISVVHVPKTIDNDYRGIDFTFGYFTAVETLAGEIRNLLADAEAGRMYFMVETMG
                     RSAGWLAYGAAIAGEASLVLSVEDLSDDVMTEETITDPATGATSRRRIMVIEKIVDQI
                     VRVIIGRETEGKEFGAIVLAEGLAQYMPAEYLEGVKFDDHGNISLSQTNLARNMTKLV
                     EAEYEKRQGKKRRVTGVQLGYEARCALPHAFDVILGSQLGVGAYRALVENRADGVLVS
                     VSGQLNLNYVPFDSLIDPDTLVTVVRFIQPGSDFQRLARSLENYPQD"
     gene            complement(1758..>2924)
                     /locus_tag="VHS97_24810"
     CDS             complement(1758..>2924)
                     /locus_tag="VHS97_24810"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX3451497.1"
                     /translation="CTGWMQATLSWGRHMNPALALTPRGEFGALVLSLSVIGPVILLT
                     FVGLDLASQEWNVSSLAIVAACVVGYGVYLGWGWSSLMRWSGTVRPASDRLRGIASRV
                     AEQIDIRPRAIEQVALPMANAFAFFSTNSIGVTDAALAVLSDNELDTICLHELAHLAE
                     SRWVRAARLSYGFVLALGIAVCGLTPGVLNSLGSVPFALLYLANIVVLITAIIWYRRL
                     VLRMEIRADALVRRFEVTPGVYAGALEKLYATNMVPVVLGSRRKTHPELFDRMITAGV
                     SPDYARPEPPPRRPLYIGLVVMLIGATLGILGLGRIAHDVPRAVLDTESAALWTIGMD
                     GGSLTELVALWNIQYDRGTLSKTPDEIDSITAGPNRSNAAGRPSPVGELGAEAE"
BASE COUNT          632 a          871 c          858 g          563 t
ORIGIN      
        1 tcgagtcgag cgcaagcacc tcggcatcta gacggcgcgc caacgccaca ccaatttcgg
       61 tcttgcccga agccgtcggt ccggtcaggt agatcgcgcg atgaaaaacg ttgtccgaca
      121 ttggcctatt cattccgaaa accgcttatt attttccgcc ttcgcccatg cgcattcgtg
      181 cggagctaaa ggcactgagg tttgttgaga aagccgttgt cgctgacttg gcgctacgtt
      241 aatgtgttga tggattccag cgggatctct tcccaccgat tctctgagct cgatcaaccg
      301 gtaaacctcg accgcgatcc agcataaaag cagagccatc atcgtacgcc aaccgttgaa
      361 acgatgggca gatcaaacga ttgaaattgg aacggagcat tccatggcgt cttccactga
      421 cacagcgtta caaccgactt tgcctgcgcg gccgatcgag cgtgtggcaa tcttgtttgc
      481 cggcggtccc gcgcccgcgg ctaacgcggt catttcgacc gcggcggcct cgtttcgtaa
      541 aatcggcatc gaagtcctgg gcatcatgca cggttacgcc catcttattg actaccgcga
      601 tgacaagccg ctccaggagg gacgcgacta catcgtcctt gatcagaaca acctcaagcg
      661 gacgcggaat acgcgcggca tcttggtcgg gacatcgcgg accaatccgg gtaaggacat
      721 ctcgcagccg gcccatctgg ccgatcctga gcgtaccgcc caacttcgtg cggtccatag
      781 agcgctcaag tcgcttggcg tgaatgcgct cgtatcaatc ggcggcgacg acacgctcaa
      841 gaccgccaac aagttcaagc ggtttcagga ctacctgccc cccgaagagc cgcgaatctc
      901 ggtggtgcac gttcccaaga ccatcgataa cgattaccgc ggcatcgatt tcactttcgg
      961 ctatttcacg gccgtcgaga ccttggcagg ggaaatccgc aatttgctgg ccgatgccga
     1021 ggcgggccgg atgtacttca tggtcgagac gatgggccga agcgctggtt ggctcgctta
     1081 tggtgcggca atcgccggtg aggctagcct ggtcctctcg gtcgaggatc tttccgacga
     1141 tgtgatgacc gaggaaacaa taaccgaccc ggcgaccggt gcgacgagta ggcgtcgaat
     1201 catggtcatt gaaaagatcg tcgatcagat tgtccgcgtg atcatcggcc gtgagacgga
     1261 aggaaaagag tttggtgcca ttgtcttggc ggagggcctg gcgcagtaca tgccggcgga
     1321 gtatctcgag ggggtgaagt ttgacgacca cggcaacatc tcactttcgc agacgaacct
     1381 ggcccgcaac atgacgaagc tggtcgaggc cgaatacgaa aagcggcaag ggaagaaaag
     1441 gcgcgtgacg ggagttcagc tcggctacga ggcgcgctgc gccttgccgc atgcattcga
     1501 cgtcatcctc gggagtcagc tcggtgtcgg cgcttaccgg gcgctggtgg aaaaccgcgc
     1561 cgacggagtt ctcgtttcgg tctcgggtca gctcaacctg aattatgttc ccttcgattc
     1621 cttgatcgat cccgataccc tggtgaccgt cgttcgcttc atccagccag gctcggactt
     1681 ccagaggctg gcgcgatcgc tcgaaaatta tccgcaggac taacgaacaa caagcgacgg
     1741 ccgtgcgggc gattcagtca ttcggcctca gcgccgagtt cgccgaccgg tgaaggccta
     1801 ccagccgcgt ttgaacggtt ggggcccgcg gtaatgctgt cgatttcgtc gggcgttttc
     1861 gagagtgtcc ctcggtcgta ttgaatgttc cacagcgcga caagctcggt aagtgagccg
     1921 ccgtccatcc cgattgtcca gagtgcggcg gactcggtat ccaggaccgc acggggcaca
     1981 tcgtgagcga tgcgccccag cccgaggatc cccagtgtcg cgccgatcag catgaccact
     2041 aacccaatgt aaagaggccg tcgcggcggg ggctcgggtc gtgcataatc cggggatacg
     2101 ccagcggtga tcatccgatc gaaaagctcc gggtgggtct tgcgccgaga gcccagtacg
     2161 acgggaacca tgttcgtcgc atagagcttt tccagcgcgc cggcatacac acccggagtc
     2221 acttcaaatc gacgtaccag ggcgtcggcc cgaatttcca ttctcagaac cagccgacgg
     2281 taccagatta ttgccgtgat cagcacgacg atattggcaa ggtacagcag agcgaacgga
     2341 accgatccga ggctgttgag caccccggga gtcagtccgc aaaccgcgat tcccagtgcg
     2401 agtacgaaac catatgacag ccgcgccgcc cggacccagc gtgactcggc cagatgcgcc
     2461 aactcatgta gacatatcgt gtccagctcg ttatcactca gaacggccaa ggccgcgtct
     2521 gttactccga tactgtttgt cgagaagaat gcgaaggcgt tcgccatcgg aagggcgacc
     2581 tgttcaatag ccctcgggcg aatatcgatc tgctcggcga cgcgcgatgc gatgccgcgc
     2641 aaccgatcag acgctggccg gactgtcccg ctccagcgca tcagactcga ccacccccaa
     2701 ccgaggtata caccatatcc gacgacgcag gccgctacga ttgcgagaga tgatacattc
     2761 cattcttggg aagccaggtc gagaccaacg aaggtcagga ggataaccgg gccgatcacg
     2821 gataaactga ggaccaaagc cccgaattcg ccgcgaggag taagcgccag cgcggggttc
     2881 atgtggcgcc cccagcttag cgttgcctgc atccaaccgg taca
//
LOCUS       DATAQL010001437         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2745538, whole genome shotgun sequence.
ACCESSION   DATAQL010001437 DATAQL010000000
VERSION     DATAQL010001437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2745538"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1138
                     /locus_tag="VHS97_24815"
     CDS             89..1138
                     /locus_tag="VHS97_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEX3451498.1"
                     /translation="MSESLNRRTFLSVSAAASLAATTLGAAPRRPVGTHRPMVISSAN
                     GLRTVEKAMELIQAGADPLDAAIEGVAIVEADPKDHSVGYGGLPNEDGVVELDAAVMH
                     GPTHGGSSVAAIRNIMHPAAVARLVMQRTRHCLLVGEGALRFARAHGFPEIDLLTEES
                     RQIWLHWKETRDPKDDWLPPPDEKVASTIREVLANRVTGTIHCSVLDSHGDLGYTTTT
                     SGLSWKIPGRVGDSPILGAGLYLDNEVGSAGSTGVGELNLLNLSSALIVEGMRRGLHP
                     KDAIMEVCKTIATRCIRDPKRRAKDGRLAGNVSFYAVSKDGRFAGGSIYPGGKMSVHD
                     GEQARVVHCDPLFAK"
     gene            1178..>3010
                     /locus_tag="VHS97_24820"
     CDS             1178..>3010
                     /locus_tag="VHS97_24820"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3451499.1"
                     /translation="MSDQETALSAGIEGTRSESRGDSLASSRRLGILMGLAVGIAAAI
                     PFIPVLSNGWVVFDDDGNFLDNVDYRGIGWAQISWAWTTFKLGVYQPLAWMLLELEYI
                     LFGLTPRGYHLTSLVLHSIVAVALYALFAKVLFRLLSPKTPGQRASVLCASSLGAAMF
                     AAHPLRVEVVAWASCQPYLQCALFSLLAVLAYLHAFDGAESQRVGWMVTAWLLFLAAQ
                     LSKAVAVMLPVVLVVLDIAVLRRIGPDNWVGPSARRVWREKLPFFAVSAIFLVLAILA
                     KGTNDSLISIQDYGPAARIAQSCYGIAFYLAKTVWPVGLAAYYPLPRPSSGLWTLPFI
                     LGIPAVVLVTLVAALLWRRRPAVLESWAAFLIILAPNMGLMRIGNQIAADRYSYVASM
                     PLFLLTAAGLYRLIHWAHRGRRRLVGIGCLGVTIVLVLGGLSWRLSSAWRDTGALWAN
                     NSRQGYVNSTVLCYLGMVEETKGDLGEAEDYYLTALRSNPQSYDAHNLLGAVLDHQGR
                     RDKAMAHYIDALRIEPEYAAAQNNLGSALARQGRLDQAIARFAEAVRLKPDFAMARKN
                     LAKALAQTGRVRKALPEFQEAARLAPNDANVRNDYGRALAQAGQL"
BASE COUNT          526 a          902 c          971 g          611 t
ORIGIN      
        1 tgacccaccc taccagacta tgatgaatca cgaaggccgc gcgacgcgcc ttgacccgac
       61 ccgttcgacc cggagggaga gagaatcgat gtccgagagt ctgaaccgcc gcacgttcct
      121 gtccgtcagc gccgccgcga gcctggccgc gacgacgctg ggggccgcgc cccgacggcc
      181 cgtcggcacg caccggccga tggtgatctc cagcgccaac ggcctgcgca ccgtcgagaa
      241 ggcgatggag ctgatccagg ccggcgccga tccgctcgac gcggcgatcg agggggtggc
      301 gatcgttgaa gccgacccca aggatcactc ggtcggttat ggcggtctgc ccaacgaaga
      361 cggggtcgtt gagctggacg ccgccgtcat gcatgggccg acacacgggg gcagctcggt
      421 tgccgcgata cgcaacatca tgcaccccgc ggccgttgcc cgactcgtca tgcagcgcac
      481 ccggcattgc cttttggtcg gcgaaggcgc gctgcggttt gcccgggcgc acgggtttcc
      541 ggagatcgat ctgctgactg aagagagccg gcaaatttgg ctgcattgga aggagacgcg
      601 cgaccccaag gacgactggc ttccacctcc tgatgagaaa gtggcatcca ccattcgcga
      661 agtgctggcc aatcgggtca caggcacaat tcactgctcg gtactggaca gccatggcga
      721 tctgggctac acaacaacga cctcgggcct ttcgtggaag atccctggtc gagtcggcga
      781 ttcgccgatc ctcggggccg ggctttatct cgacaatgag gtcggctcgg cgggttcgac
      841 gggagtgggt gagctgaacc tgctaaacct ctcgagcgcc ttgatcgtcg agggaatgag
      901 gcgtggatta catcccaagg acgcgatcat ggaggtctgc aagacgatag ccacgcggtg
      961 catccgagac ccgaagcggc gggccaaaga tggacgactc gccggcaatg tgtcgttcta
     1021 cgccgtgagc aaggacggga ggtttgccgg tggcagcatc tatcccggcg gcaagatgtc
     1081 cgttcacgac ggcgaacagg cccgagtcgt tcattgcgat ccgctgttcg cgaagtaatt
     1141 ggcgtgggcc tctagggggg cactcgatca aacttttatg agcgatcaag agaccgcgtt
     1201 gtcggccgga attgagggga cccgctcgga atcgagaggc gattcgctcg caagttcgcg
     1261 gcggctgggc atcctcatgg ggctggcggt gggcatcgcg gctgctatcc cgttcatccc
     1321 tgtcttgtcg aatggctggg tcgtcttcga tgacgatggt aattttctcg acaacgtcga
     1381 ctaccgcggg atcggctggg cacagatatc ctgggcctgg acaacgttca agctcggtgt
     1441 ctaccagccg ctcgcctgga tgttacttga gcttgaatat atcctcttcg ggctcacacc
     1501 tcgcgggtat caccttacga gcttggtgct tcactcgatc gtggcggtgg cgttatatgc
     1561 acttttcgcc aaggtgctct ttcggctgct gtccccaaaa actcctgggc agcgcgcgtc
     1621 ggtgttgtgc gcgtcatcgc tgggcgcggc aatgttcgcc gcgcatccgc tacgggtcga
     1681 ggtcgtcgct tgggcatcgt gtcagcccta tttgcaatgt gcactcttct cattgctcgc
     1741 cgtacttgca tatcttcatg cattcgacgg ggcagagagc cagcgtgtcg gctggatggt
     1801 aacggcatgg ttgttgttct tggcggccca actgtcgaag gcggtcgcgg tcatgttgcc
     1861 ggtcgtgctg gtggtgctcg atatcgccgt attgcggcgg atcggccccg acaactgggt
     1921 cggccctagt gcgcggcggg tctggcgaga aaagctgccg ttcttcgcgg tcagtgcgat
     1981 ctttctggtc ctcgccattc ttgccaaggg gaccaacgac tcgttgatct cgattcaaga
     2041 ctacgggccc gcggcacgca tcgcgcaatc ctgctatggg atcgcgttct atctcgccaa
     2101 gaccgtctgg cccgtgggcc tggcggcgta ctaccccctg ccccggccgt cgtcagggct
     2161 ctggacgctg cccttcatcc ttgggatacc agcggtcgtg ctggtgacgc tggtcgcggc
     2221 acttctctgg cgccgacggc cggctgtgct cgagagttgg gccgcatttt tgatcatcct
     2281 ggcgccgaac atgggactga tgcggatcgg caaccagatc gccgccgatc gatacagcta
     2341 tgttgcctca atgcctctct tcctgttgac agccgccggg ctctatcgtc tcattcattg
     2401 ggctcatcgc gggagacggc gcctggtcgg gatcggctgc cttggtgtga caatcgtttt
     2461 ggttctgggc ggtttgagtt ggcgtctctc tagcgcatgg cgagacaccg gtgcgctctg
     2521 ggcgaacaac tctcggcagg gttacgtgaa ctcaacggtc ctctgctatc tgggtatggt
     2581 cgaggaaaca aaaggggatc tcggagaggc cgaggactac tatctaacgg ctttgcgctc
     2641 taatccgcaa agctacgatg cccacaacct actcggggct gtcctcgatc accaaggccg
     2701 gcgtgacaag gcgatggcac actatatcga cgcgttacgg atcgagccgg aatacgccgc
     2761 cgcgcagaat aatctcggat cggcgctggc ccgtcagggg cgactcgacc aggcgatcgc
     2821 cagattcgcc gaggctgtgc gtctcaagcc ggatttcgcg atggcacgaa agaacctggc
     2881 gaaagccctg gctcagaccg ggagagtgcg taaggcactg cccgagttcc aggaagcggc
     2941 gcggcttgcc ccgaacgatg cgaacgtgcg caacgactac gggcgtgccc ttgcccaggc
     3001 tggccagctt
//
LOCUS       DATAQL010001438         9601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5187319, whole genome shotgun sequence.
ACCESSION   DATAQL010001438 DATAQL010000000
VERSION     DATAQL010001438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5187319"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="VHS97_24825"
     CDS             <1..1703
                     /locus_tag="VHS97_24825"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX3451500.1"
                     /translation="RSQALINGGLIDRPALELNTYYVLTLNHDNTITVVDPLFSFGGT
                     KLLALVPLPAGGEDWMFGPGARQVFVALAETNQVAIVDTQSWKITASVPAGIRPHRLA
                     LQPDGRYVWVAGGVEGHADSGVTVLRESDGSIAARIRTGPGRHDLALSDDSRHAFVTN
                     TEAGSVAVVDALALRTVATIPTGRRPTSIAYSAHAAMAYVTDAVDGTISAIDPVAGRL
                     VNRITAEPGLGQIRFSSTGGAGFVINTQRSLLHVLDPASNRIVQTAQLEAGPDQIAFS
                     SDFAYIRHSGSVNVVMVSLKTAGKAGAPLSVLRFPAGERPPGSQNGGIPADSIIPAPG
                     ARAMLVANAADKSVYYYKEGLAAPMGTFSNYKREPRAILVLDRSMRERAEPGVYETVA
                     RLDRAGRFDVIFFLDQPRIAHAFPFEVEADPALQLARKRGKVKIEPILAVNEVDAGKT
                     LRPLFQLIDLESGTVKSGVKNLEILMFRVGGGWQDRRPAREIAAGIYGAEFVPDRPGV
                     HEIFVVLDSAGLKAEGPRGLSIRVVETAAGGPGDLRALVAHPQSAGTATAEKDGSYDQ
                     "
     gene            1700..2971
                     /locus_tag="VHS97_24830"
     CDS             1700..2971
                     /locus_tag="VHS97_24830"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3451501.1"
                     /translation="MSTRALGLGLICCTGLAVAALVGFGSVCLRPGPSQAVDADARAD
                     CCAPTDGPDRSDSAVPLAAANADAAEDSRPIKVPDVELVDQHGRAVRFYSDLVKGRLV
                     AINFIFTTCKGVCPPIGATFGKLSQGLEDQGVHLISVSVDPVNDTPERLAAWAGKFGA
                     QSNWTLVTGTKQDVDGLLKGLGVFSSDKANHSPLILLGNDRALTWRRIHGLSPLETIR
                     AAIDTVRKAPGLEASAAVTDAKPAGEPISPAHRYFTDVSLVNQYGERMRLYTDLIRSK
                     VVVIHVFFASCKNTCPLLATTFQKLQEHLGDRLGRDVHLISITVDPQSDDQKVLEDYS
                     RRLGARQGWYLLTGPSRDVETALTKLGLAVARRDEHSNIFLIGNEKTGLWKKVRGLGP
                     ADSIIEVLDGVIADKGEPSAQVPAPVQGGGD"
     gene            2975..4639
                     /locus_tag="VHS97_24835"
     CDS             2975..4639
                     /locus_tag="VHS97_24835"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3451502.1"
                     /translation="MRDSRRLLWLAGVLYVLAVSAGGCRPEASATTPTPVGPPSDAEA
                     RPAITRDEDDRALSQRRGRRIYFEGTSSSGSSIVAVIGSSREGVSAALVTCGNCHGRD
                     GRGRPEGGIEPRDVRWSVLSKPLAATERPGRPAYTRAVAGRAITMGLDAGGARLGDGM
                     PRYRLSPEDLADLVAYLEVLGSEVDPGISPTSITLGTLLPQEQSAPELRQAIVSTVQA
                     FADDLNDRGGLYNRKIVIQFEDMNPAPSGSGRDVEELLKRGPMFALLAPYFAGRDQEL
                     ARVVRDEGLPVIGPFSNLSSSGAAAERCIFHIHSAIDDQARALAIFAIGQPRARAPAI
                     IESDDRLSPQAADAVAQVWARAGWSVPERIVIPGGEEGAQVTRLVERLAARGNDVIVY
                     LGPAALVQPLLVAADGASYRPSVFLIGALSGGGVLDLPASFDGRAFLAMPHLDQDVTR
                     SGHEDYAHLSARYKLVPDHRAAQLAVLAAARVLEEGLRRCGRDLSRAKLIDELERLEE
                     FPTGYSRPVTFGPNRRAGTAGAHVLGADLVEHRFKPLGWTDVGRRQ"
     gene            4719..5453
                     /locus_tag="VHS97_24840"
     CDS             4719..5453
                     /locus_tag="VHS97_24840"
                     /inference="COORDINATES: protein motif:HMM:NF016377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451503.1"
                     /translation="MSSNRIQGLWVGRRLSFMERLSIASFLHHGHEYHLYTYGPVEGV
                     PEGAVVLDGGSILPESMIFQYREHASYAGFSNYFRYKLLLERGGWWVDTDTICLKPFD
                     FDEPYVFASQPTKDGREIPTASPIKAPAGSPAFAYAWDYCLSREPQDLVWGETGPRLV
                     AKAIERFSLERFRHSREVFCPLDPGEWKRVVDPAALWRFEESSRAVHLWNELWRKEGW
                     DKDRDYPKGCLYECWKEQFLAARPTN"
     gene            5548..6498
                     /locus_tag="VHS97_24845"
     CDS             5548..6498
                     /locus_tag="VHS97_24845"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3451504.1"
                     /translation="MKLVIAGLRALDGYVSREFSFIIRELIDQYGWKHLEFAALRHSG
                     KPVADALQEACGELPRVVLFWEGYDFLCAHAREVQALPCLKGIFADDIHDKTIVTRWG
                     RIGAYLSCDIVFAAYADRFEELFPEVCRLMRVVWTPHSASPEFLLPWNPRARNAVLLS
                     GMVNEFYPLRQAMKSLCDTGRQAIVHHPHPGYHCGYRRDDSRVGRAYAQLLNGFRTAF
                     TDSSRFRYIVAKFFEIPAAGALLLADRNAEPSLTKLGFKAGVHYVPATFKDLESQVEF
                     VLDRSNRDALDQIRLRGQRLVWKRHTTSNRARLIDETCRM"
     gene            7142..8182
                     /locus_tag="VHS97_24850"
     CDS             7142..8182
                     /locus_tag="VHS97_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451505.1"
                     /translation="MNRRAFTLIELLVVIAIIAILIGLLLPAVQSAREAARRAQCTNN
                     LKQIGIAVHNYEGSFGSYPLGTIIAIWPSDPALSQGNYRWGTLAFLTPFFEQTSVFNS
                     LNFSFPLYGEPVPVLSSQVYPANRTSVNVMASLFLCPSDRMERLETADGFLGGEGRQF
                     APTNYQFCAGSGSGGGDVTVADGVFRVNVITRHQAITDGLSNTAFASESLLGSGGVRT
                     ILPNAGPFDPKVLFASVSWQGSAPLSITPTACANPISYSPNRLFTWADGSYSDGLYNH
                     YYPPNTLLMDCIVGINGVNDGWKAARSRHPGGVNVLYGDGSVHNVKDSINSLVWAGIG
                     TRAGGEVATDTQ"
     gene            8358..9512
                     /locus_tag="VHS97_24855"
     CDS             8358..9512
                     /locus_tag="VHS97_24855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3451506.1"
                     /translation="MKAADWHLALPTGLSGLGLLITLILGFWMARRPKMPFVLLPGFI
                     SCAVCLGMIVSPRSLWFFFFLGLFNLFETITRPAITAIIRTNYPVATRGLVTGRLRQW
                     SAGLFLASALGTAQVLDRSGSWAVIQVILLTAATLQVGAYIAFSFIRVQPESADIDEE
                     ASEQDLWTFAQSTSVTLRQDSRFLRYLSGCFLHGVSALTYDPIVRAYFSNEFGFNYTQ
                     CVLLVDFLPSVCSVLTVGYLGAWFDRTNPLVAWTFIRAAWGLDPLLLALAPSYPAGSL
                     PIALAARISRGSVMNGSWVLGWQLATNYFARRRELTSVYMACYLTVTGIQRLIGPPLG
                     ALLLGVFSRREVLFIGGFFVMLSSLHAWRQAASERIDGRYPTFADKESDV"
BASE COUNT         1700 a         2945 c         3054 g         1902 t
ORIGIN      
        1 tgcgctctca ggcgttgatt aatgggggct tgatcgaccg cccggccctg gaactgaata
       61 cctactacgt gctgacgctc aaccacgaca acacgatcac ggtggtcgac ccgctgttca
      121 gcttcggtgg cacgaagctg ctggctctcg taccgctgcc ggcgggcggc gaagactgga
      181 tgttcgggcc cggggcacgc caggtcttcg tggctttggc tgaaacgaat caggttgcca
      241 tcgtcgatac acagtcgtgg aagatcaccg cgagcgtgcc ggctggtatc cgcccgcacc
      301 gcctggctct gcagccagat ggccggtatg tctgggtcgc cggcggcgtg gagggccacg
      361 ccgactcggg cgtaaccgtt ctgcgggaga gcgacggcag catcgccgcc cggatccgca
      421 ccggtccggg ccgacatgat cttgcgctca gcgacgacag ccgccatgcc tttgtcacga
      481 acaccgaggc aggctcggtc gcggtcgtcg acgccctggc ccttcgcacg gtggcaacga
      541 tcccgactgg ccggagaccg acgtcgatcg catactctgc tcacgcggcg atggcctacg
      601 tgaccgatgc ggtggacggg acgatcagcg ccatcgatcc ggtggccggc cggctcgtca
      661 atcgcattac ggccgaaccg gggctggggc agatccgatt cagttcgacc gggggcgccg
      721 ggtttgtgat caacacccag cggagccttc ttcatgtgct cgatccggcg agcaaccgca
      781 ttgtccagac cgcccaactg gaagcagggc ccgaccagat cgcgttctca agtgattttg
      841 cgtacatccg gcacagcggc agcgtcaacg tcgtgatggt ctcgctcaag actgcgggga
      901 aggcgggcgc cccgctgtcg gtccttcggt ttcccgcggg ggaaagaccg cctggatcgc
      961 agaacggcgg gataccggcc gattcaatca tcccggcccc gggcgccagg gccatgctgg
     1021 tggcaaacgc cgctgacaag tccgtctatt actacaaaga gggcttggcc gctcccatgg
     1081 gaacgttcag caactacaag cgcgagccgc gggcgatcct ggtgcttgac cggagcatgc
     1141 gcgagcgtgc agagcccggt gtgtatgaga ccgtggcgcg gctcgaccgt gccggccggt
     1201 tcgacgtgat cttcttcttg gatcagccgc ggatcgcgca cgctttccca tttgaagttg
     1261 aagccgaccc ggcgctccag ctcgcacgaa agcgggggaa ggtcaagatt gagccgatct
     1321 tggccgtgaa cgaggtcgac gcggggaaga ccctccgccc gttgttccag ctcatcgacc
     1381 tggagagcgg caccgtgaag tccggagtca agaatctcga gatcctgatg tttcgagtcg
     1441 gcggcggctg gcaggatcgg cggccggcgc gcgagatcgc tgcgggcatc tacggcgcag
     1501 agtttgtgcc ggaccgaccg ggcgttcatg agatctttgt tgtcctcgac tcggccggcc
     1561 tgaaggcgga gggcccgcgg ggcctttcga tccgagtggt cgaaaccgcc gcgggcgggc
     1621 cgggggacct gagggccctc gtcgctcatc cgcaatcggc cgggacggcg accgcagaga
     1681 aggatgggag ttacgatcaa tgagcactcg tgccctcggc ctgggtctga tctgctgcac
     1741 cgggctcgcc gtcgcggcgc tggttggttt cggatcggtc tgcctgcgac ctgggccgtc
     1801 gcaggcggtc gacgccgatg cacgggcgga ctgctgtgcc ccgacggacg gtccggatcg
     1861 aagtgactcg gctgtgcctc tagcggcggc gaatgccgat gccgcagaag attcccggcc
     1921 gatcaaagtc cccgatgtcg agctggtcga tcagcatggc cgagcggttc ggttttacag
     1981 tgacctggtc aagggccggc tggttgcgat caacttcata ttcacgacat gcaagggcgt
     2041 gtgcccgccg atcggggcga cgttcggaaa gctctcgcaa gggctcgagg atcagggcgt
     2101 ccatttgatc tcggtgagtg tcgatcccgt gaacgatacg cctgagcgcc tcgccgcctg
     2161 ggccgggaaa tttggcgctc aatcgaactg gacgcttgtc acaggaacaa agcaggacgt
     2221 cgacgggctg cttaagggcc tgggagtctt tagctcagac aaggccaacc attcgccgct
     2281 gatcttgctt ggaaatgatc gcgccctaac ctggcggcgg attcacggcc tctctccgct
     2341 cgagacaatc cgggccgcga tcgatacggt acgtaaggct ccgggtctcg aggcgagcgc
     2401 ggccgttacg gacgcgaaac cagcagggga accgatctcg ccggctcatc ggtacttcac
     2461 ggacgtgagt ctggtgaacc agtacggaga acggatgagg ctttacaccg acctgatccg
     2521 ttcgaaggtg gttgtgatcc acgtcttttt cgcctcgtgt aagaacacgt gtccgctcct
     2581 ggcgacaacg tttcaaaagt tgcaagagca cctgggcgat cgcctgggac gcgacgttca
     2641 cctcatctcg atcacggtcg atccgcaaag cgacgatcag aaggtgcttg aagattactc
     2701 gcggcggcta ggcgcgcgcc aaggctggta tcttttgacc ggaccgagcc gcgatgtcga
     2761 aaccgctctc acaaaactcg gtctggcggt tgctcgccga gacgaacatt cgaacatttt
     2821 cttgatcggc aatgagaaga cggggctctg gaagaaagtc cgcggtctcg gccccgcgga
     2881 ttcgatcatc gaagttctgg acggagtgat cgccgataag ggcgaaccgt cggcacaggt
     2941 gccggcgccc gttcaaggag gaggggattg atcgatgcgg gactcgcggc ggcttctctg
     3001 gctcgcgggc gtcttgtacg tcctcgcggt ttcggcgggg ggctgtcggc ccgaggcgtc
     3061 ggcaacgacc cccactcccg tggggccgcc ttccgatgcg gaggctcgtc cggccattac
     3121 gcgcgatgag gacgaccgtg ccttgagcca gcgccgcggt cggcggattt attttgaggg
     3181 aaccagctct tccggcagct caattgtcgc ggtgatcggg tcgtcgcgcg aaggcgtgtc
     3241 cgccgccctg gtcacgtgcg gcaactgtca tggccgcgac ggccgcgggc ggcccgaggg
     3301 ggggatcgag ccacgcgacg tgcggtggtc ggtgctcagc aagccgcttg ccgcaacgga
     3361 gcgccctggc cgcccggcat acaccagagc ggtcgccggg cgggccatca cgatgggcct
     3421 cgatgccggg ggcgctcgct tgggtgacgg aatgccccgc tatcggctct cgccggaaga
     3481 cctggcagac ctggtcgcgt atctcgaagt gctgggctcg gaggtcgatc cgggcatctc
     3541 gcctacgagc atcacgctcg gcacgcttct accccaggag cagagcgcgc ctgagctgcg
     3601 gcaggcaatc gtttctacgg tccaagcttt cgcagacgac ctgaatgatc ggggcggatt
     3661 gtacaatcga aagatcgtca ttcagtttga agacatgaat cctgcaccga gcggatcggg
     3721 ccgggatgtc gaggagctct tgaagcgagg cccgatgttc gcgctcctgg cgccttactt
     3781 cgcaggtcga gaccaggagt tggcgcgagt cgtccgcgac gaggggcttc cggtcatcgg
     3841 cccgttctcg aacctgtctt caagcggggc tgcggcggag cgatgtatct tccatatcca
     3901 ctcggcgatc gacgaccagg cgcgtgcgct cgcaattttc gcgatcggtc agccccgcgc
     3961 cagagcaccg gccattatcg agagcgacga tcggctgtct ccgcaggcgg cggacgccgt
     4021 ggcccaggtc tgggcgcgcg ccggatggag cgtaccggaa cgaatcgtaa tcccgggagg
     4081 cgaggaaggc gcccaggtaa cgcggctggt cgagcggctg gcggcgcgcg gtaacgacgt
     4141 cattgtctac ctcggaccgg ccgcgctcgt tcaaccgctc ctggttgcgg ccgacggcgc
     4201 ctcttaccgc ccgagcgtct ttttgattgg cgctctctcg ggcggaggag tcctcgatct
     4261 ccccgcgtcc ttcgacggtc gcgccttcct ggcgatgccg cacctcgacc aagacgtcac
     4321 acgatccggg cacgaagact acgcccatct gagcgctcga tataaactgg ttcctgacca
     4381 ccgggccgcg cagcttgcgg tccttgcggc tgccagggtc ctggaagaag ggcttagacg
     4441 ctgtggccgg gacctgagcc gggcgaagct gatcgacgag cttgagcgac tggaggagtt
     4501 tccgaccggc tattcccgcc cggtgacatt tggaccgaac cggcgtgccg ggacggcggg
     4561 ggcgcatgtg cttggtgccg atctcgtcga gcaccgcttc aaaccgctcg gatggaccga
     4621 cgtggggcgc cgtcaatgag caatgtggtg aagcgaccga gagctcggcc ccgcgccggc
     4681 acaatctaac gtacttcctg tgaagggtcg aacgcggcat gagcagcaat cgcattcagg
     4741 gcctttgggt cggtcgtcgc ttgtcattca tggagcgtct ctcgatcgcg tcgttcttgc
     4801 accacggaca cgaatatcat ctctatacgt acgggccggt cgaaggtgtg cccgagggcg
     4861 ccgtcgtcct tgatggcggc tcgatccttc cggagtcgat gatttttcag taccgagaac
     4921 acgccagtta tgcgggattc tcgaactatt tccgctataa gctgctgctc gagcggggag
     4981 ggtggtgggt cgacaccgac accatctgcc tgaagccatt cgacttcgac gagccgtacg
     5041 tttttgcttc gcagccgacc aaggacggtc gcgagattcc gacggcgtca cccatcaagg
     5101 cgcccgcggg aagcccggcc tttgcctatg cctgggacta ctgcctctcg cgcgagccgc
     5161 aggatctcgt ctggggtgag actggtccgc ggctcgtcgc caaggccatc gagcgattct
     5221 cgctcgagcg attccggcat tctcgcgaag ttttttgccc gcttgatccg ggcgagtgga
     5281 agcgggttgt tgaccccgcc gccctatggc gatttgagga gtcttcccgg gctgtccatt
     5341 tatggaacga attatggagg aaagaggggt gggacaagga ccgcgactac ccgaagggtt
     5401 gcctgtacga gtgctggaaa gagcagtttc tcgcggcgcg gccgacgaac tgagatcgtg
     5461 cgcccgttcg cgcatgtcca gaatcgcctt accttcaacc gttcgagcga cgtcaggagg
     5521 ttgctcactc gcgatcttgg agccgctatg aaactggtca tcgccgggct cagggctctg
     5581 gacggctacg tcagccggga attctcattc atcatcagag agttgatcga tcaatacgga
     5641 tggaagcacc tggagttcgc cgcactgcgg cactcgggaa agcccgtggc ggatgccctt
     5701 caggaggctt gcggcgaatt gccacgggtc gttcttttct gggaaggtta cgacttcctg
     5761 tgcgcacatg cgcgagaggt ccaggcgctt ccctgcctga aggggatatt tgccgatgat
     5821 attcacgaca agaccatcgt gacacgatgg ggcaggatcg gggcgtacct gagctgcgac
     5881 attgtctttg cggcttacgc cgatcggttc gaggagttgt tcccggaggt gtgccgactg
     5941 atgcgggtcg tgtggacgcc gcactcggcc tcgcccgaat ttctgttgcc ctggaaccca
     6001 cgagcccgaa acgccgttct cctcagcgga atggtcaacg agttttatcc gctacgacag
     6061 gcgatgaagt cgttatgcga cacgggccga caggcgatcg tccaccatcc gcatcctggc
     6121 tatcactgtg gctatcggcg tgacgactcg agagtgggaa gggcttacgc tcagttgttg
     6181 aacgggtttc ggaccgcgtt cacagacagc tctcgattcc gctacattgt ggcgaaattc
     6241 ttcgagatcc cggccgccgg cgcgctcttg ctggcagacc gcaatgccga gccatctctc
     6301 acaaaactcg gcttcaaagc gggcgtgcac tacgtgccag cgaccttcaa ggacctggaa
     6361 agtcaggtcg agttcgtgct cgaccgatcc aaccgagatg cgctcgatca gatccggctc
     6421 cggggccagc gcctggtgtg gaaacgccat accacgagca atcgagcgcg gctcattgat
     6481 gagacttgcc ggatgtgaac gagccatcga ttgcgatcga tttttcgatc gaaaacccgc
     6541 cgagataaac cggtaacggt tgatctgtta gccttggttg aattaatcgt cagatctggc
     6601 gactaccatc tggaggaaag cccagcggca cattaaactg atttcgcccg atcagtccac
     6661 tccgatgaca gagacgtcca cggggaactg atcacgccaa aaatggactt cattcacctc
     6721 gtcttttttg tctcaagccg ctggaccctc cgacgaacga tcgcggctct caatcagcga
     6781 aaacggcctt ggcaagggca cgattgcacc ggtcgacgaa taaggacttc gtgatgttac
     6841 gtcgcggatc ccgtttccat ccctgtgtcg tttacaaagc ctgtgggctg gcgattttgc
     6901 tctgttcgca cggatgcagc tcggaaccgc aatcaccggc tgcgcagggc gctctaccgg
     6961 tggaagtcac ctgcgactcc tgcggaaaga aggtcgcccg gacggacacg gagtcgcaca
     7021 tcagccttga gggattggac gtaaacgtct gccgggcctg cgcgggcaaa gcagcaaagg
     7081 gccggccggc atctccgccg ccgggtcgag cttcggccaa aacttccaaa cgggggacgt
     7141 catgaatcgg cgagcattca ctctgattga gctgctggtg gtgatcgcga tcatcgcgat
     7201 cctgatcggg cttttgcttc ccgcggtgca atcggcgcgg gaagcggctc gtcgtgccca
     7261 gtgcaccaac aacctcaagc aaatcggtat cgcggttcac aactacgaag gctcgttcgg
     7321 cagttatccg ctgggcacga tcatcgcgat ctggccgagc gatcccgctc tctcacaggg
     7381 caattaccgg tggggaacgc tcgcgttcct cacgccgttt ttcgagcaga caagcgtttt
     7441 caactcgctg aatttctcgt ttccgcttta cggagagccg gtcccggtgt tgtcgtccca
     7501 ggtttatccg gccaatcgga cgagtgtcaa tgtcatggcc tcgctgtttt tgtgcccgag
     7561 cgatcgcatg gagagattgg agacggccga cggattcctg gggggagagg ggcggcagtt
     7621 cgctccgaca aactaccagt tctgcgctgg gagcggttca ggcggcggtg acgtgaccgt
     7681 ggcagacggc gtcttccgtg taaacgtcat cacgcgacac caggcgatca ccgacggtct
     7741 ctcgaatacc gccttcgcca gcgaatcgct gctggggtcg ggaggcgtgc gaaccattct
     7801 gccgaatgca ggcccgttcg acccaaaggt cctcttcgcc tccgtgtcgt ggcaaggctc
     7861 ggcgcccttg agcattactc caactgcctg cgcgaacccg atctcttact ctcccaaccg
     7921 actgttcacc tgggccgatg gtagttacag cgacggcctg tacaaccatt actatcctcc
     7981 aaacacgttg ctcatggact gcatcgtcgg aatcaacgga gtcaacgatg ggtggaaggc
     8041 ggcgcgcagc cgccatccgg gcggcgtcaa cgtgctctac ggtgatgggt cggtgcacaa
     8101 cgtgaaagac tcgatcaaca gtctcgtctg ggcggggatc gggacgcgcg ccggcggtga
     8161 ggtcgcgacg gacacccaat gaacctctga gcgcggcgta tcgcatcgat gattcgaatc
     8221 atcggagaac gaccctggaa gctccagacc tcccgatcga agcccgtcgg acctattggt
     8281 tccattgggt ctactccgtc ctgggtggga tcacctccgg tatcctgacc aatgccccga
     8341 ccatcggcat caaggtcttg aaggcggccg actggcatct cgccttgcct acgggcctct
     8401 cggggctcgg actgctcatt acgctgatcc tgggcttctg gatggccaga cgacccaaga
     8461 tgccattcgt cctgctcccg ggcttcatct cctgcgccgt ctgcctgggg atgatcgtct
     8521 cgccccgatc gctgtggttc ttcttcttcc tgggcctctt caacctgttc gagaccatca
     8581 cgcgccccgc gatcaccgcc ataatccgca cgaattatcc cgtcgccacg cgcggcctgg
     8641 tgacgggcag gctccgccag tggagcgcgg ggctcttcct cgcgtcggcc ctcggcacgg
     8701 cgcaggtgct ggatcggtcc ggatcctggg cggtcatcca ggtcatcctg ctgacggcgg
     8761 caacgctcca agtcggcgcc tacatcgcct tcagcttcat ccgtgtccag cccgagtcgg
     8821 ccgacatcga cgaggaggcc tcggaacagg acttgtggac cttcgcgcag tccacctcgg
     8881 tgaccctgcg gcaagactct cggttcctcc gctacctgtc cggctgcttc ctgcacgggg
     8941 tgagcgcgct gacgtacgac cccatcgttc gcgcctattt ttcgaacgag ttcggattca
     9001 actatactca atgcgtcctg ctcgtcgact tcttgccgag cgtctgttcg gtcctgaccg
     9061 tcggctacct gggcgcgtgg ttcgaccgga ccaatccgct ggtggcgtgg accttcatcc
     9121 gcgccgcctg gggtctggac cccttattgc tggcgctggc gccgtcctac cccgccggct
     9181 cgctgccgat cgcgctggcc gcacggatct cgcgcggcag tgtgatgaac gggtcgtggg
     9241 tcctgggatg gcagctcgcc accaattatt tcgcgcggcg ccgcgagttg acctcggtct
     9301 acatggcgtg ttatctcacg gtcacgggaa ttcagcgcct gatcggcccc cctctggggg
     9361 cgctactgct cggcgtcttc tcgcgccgcg aagtgctctt cattgggggc ttcttcgtaa
     9421 tgctgtcgtc tctccacgct tggcggcagg ctgcatcgga gaggatcgac ggtcgctatc
     9481 cgaccttcgc cgacaaggaa agcgatgttt agatccaaaa caagcataac atattaaaca
     9541 gtcgacgacg aattaaatca tgtttatatt ttagttaaat gcgcaccttc ttcggtcgca
     9601 a
//
LOCUS       DATAQL010001439         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4880854, whole genome shotgun sequence.
ACCESSION   DATAQL010001439 DATAQL010000000
VERSION     DATAQL010001439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4880854"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VHS97_24860"
     CDS             <1..648
                     /locus_tag="VHS97_24860"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX3451507.1"
                     /translation="IDDFNGENFDHSGLGFFGGGYIQCGHAGAPPIAGRAVPRGTPTW
                     GSEWKHATVKWYHHFTRFNTQGSVYASRHNYMDLDPTYKDAFGRPMIRLTYNGTENDH
                     AMSRYLLAKLEGVIKAMNPTHYELRPRPRNFTIVPYQSTHNTGGTIMGADPGSSVVNR
                     YLQAWDAHNLFIQGASVFPQQHGYNPTGTVGALAYWSADAIVSQYLKRPGPLVHA"
     gene            2028..>2485
                     /locus_tag="VHS97_24865"
     CDS             2028..>2485
                     /locus_tag="VHS97_24865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451508.1"
                     /translation="MSCNRITRLHMAAFFAMLSLIVAGGAPAAGQAAQLPERPEQHQH
                     GHDLNIPSGVTDNCEPAYTYDEGPVGPSHWGGVCNAGHLQTPIDISKPDKVSLALLPQ
                     LLFGYEPSQLDMVNDCNHYQLKLRFPPNHWLKVAKKPYRLSEVRFREPGET"
BASE COUNT          553 a          757 c          703 g          472 t
ORIGIN      
        1 atcgatgatt tcaacggcga aaatttcgat cactccggcc taggcttctt cggcggcgga
       61 tacatccagt gcggccacgc cggcgccccg ccgatcgccg gccgcgcggt tccacgcggc
      121 acaccaacct ggggctccga atggaagcat gcgacggtga agtggtatca tcacttcacg
      181 cgcttcaaca cccagggatc ggtctacgcc agtcggcata actacatgga cctcgatccc
      241 acctacaagg acgcgttcgg ccgtccgatg atccggctga catacaatgg gaccgagaat
      301 gatcacgcga tgtcgcgcta cctgctggcc aagctcgagg gcgtcatcaa ggcgatgaac
      361 ccgacccatt acgagttgcg gccgcggccg cggaatttca ccatcgttcc ctaccagtcg
      421 actcacaaca cgggtggcac catcatgggc gccgatccgg ggtcgagcgt ggtcaatcgc
      481 tacctgcagg cctgggacgc ccataatctg ttcattcaag gggcgtcggt attcccgcag
      541 cagcacggct acaatccaac cggcaccgtc ggcgcgctgg cctactggtc cgcggatgcg
      601 atcgtatcgc aatatctgaa gcggcccggt cccctggtgc atgcgtaaac cgaaaccggg
      661 atcaagtaaa aagccgcacc aggagcatag ggaccacctc aaagaggccc gacccgcggc
      721 ctcgtgacct tgatttcacc gcggataaaa gatgcgcgcg gttcgtaggg atcgatagct
      781 gccggcctat tgtgagcgat ccctccgagt tgggcagcaa gctccgcgag gtcctgcggg
      841 tgtcgagatc gcctggagcg gtggtcgccg tcgtaggtcc cgtgtccgaa ggtccgcttg
      901 cccgacgggt aacggtggcg acgagggggt gtgcgcgccg gcgaccggtc gaaatcgcgg
      961 tagaactgtt agcttcatcc gcggggcgac gcccgccggg gatttgaagc cggcccataa
     1021 taccggtcga tcccgcttgt ccaagttagg cggaacagtg tgtcctggtg tcacgctcgg
     1081 cgaaaatgac aacgacgatg atgcagacgg gcaggagagc accaatccag gccgtcgagc
     1141 caagcaatat ccgctcaaag cactcactcc gcccgaagta cccgtgccgc ccaggtgcga
     1201 cgcaaacacc ccctccgact tccccgtatc tgcggcgcac cggggcgtga ggcgaacgat
     1261 attcgttggt tatggattgc gctccgcccg gtgccgcgaa ggctcactcg aacgcaacag
     1321 gcctcggcgc tggagcgtgg cgaggcgtcc atcggtatca ggcagaaaag ccggcttgtg
     1381 gctggaaagc tgacggctga catttttggc atgcttgtgc tgcaggtgcg ccgtgtccac
     1441 gagccgcatt gacctcgccg acggtcttgt gcaggggcca cggcaggtct gttggctctc
     1501 gaagacattc aaaaccgagg catcggctgg attgccagaa gagcagatgt cagggagtga
     1561 attaccgttc ttttgcgaaa gaaccccgcg tctacacttt caccattcaa cctcgccagc
     1621 aaaactacgt cccacgacta tattcatgga gaactaatac ctgaagatcc gcaccacgta
     1681 aacgaggatg gtttctaaaa acgccacaag tcttgacgat tgcggtattt tcgctcgttt
     1741 caaggtcatc catcgccgtc acaacaccgg ttcaaacgga aggcccgcac taacgatgcc
     1801 gcttttcacg catacacttt tcatcgcttt gccgcaatcg acatgatcaa ccacgagaaa
     1861 actttattgc agtttcgtga cgatcgaata aggtggtcgg cattctcgca cgactacact
     1921 aaaaaaccat gtcttggtag agtgatgccg gtgatttaga gccaacaata aagataaggt
     1981 cagagcgcca cacggcttcg cgaagaccct aacaaccgag taggaacatg tcctgcaacc
     2041 gaattacgcg ccttcacatg gcggcattct tcgcaatgtt gtcgttgatc gtcgccggcg
     2101 gcgcgcccgc cgccggccag gcggctcagc ttcctgagag acccgagcag caccagcacg
     2161 ggcatgacct gaacatccct agcggagtca ccgataactg cgaacccgcg tacacctacg
     2221 acgagggacc agtcggaccc agtcattggg ggggtgtttg caatgccggt cacctgcaaa
     2281 cgccgatcga tatctcaaag ccggacaaag tttcgctcgc gttgcttccg cagctcctgt
     2341 tcggctatga gcctagtcag ctggacatgg ttaatgactg caatcactac cagctcaagc
     2401 tcagatttcc tccaaaccac tggctcaaag tcgcaaagaa accttatcga ctgagcgagg
     2461 tccgttttcg cgaaccgggc gagac
//
LOCUS       DATAQL010001440         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5340535, whole genome shotgun sequence.
ACCESSION   DATAQL010001440 DATAQL010000000
VERSION     DATAQL010001440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5340535"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VHS97_24870"
     CDS             <1..549
                     /locus_tag="VHS97_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX3451509.1"
                     /translation="PRQIDDARSAAAMALAEAKHPQHGSPLFPQIIETAAAYQIDPTR
                     EGYPDLIALPDEPYWVRTKLTSSVSWVEPDPNLPGTHRPEGIVALGAAGLAPGRYLNA
                     HLIDATPTILALLGQAIPAHIEGKPIVGTAATPHETPCFAPRRHDGAEQLLEGPHKKP
                     FEYSSEEQEIIEQRLADLGYLE"
     gene            complement(582..1073)
                     /locus_tag="VHS97_24875"
     CDS             complement(582..1073)
                     /locus_tag="VHS97_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX3451510.1"
                     /translation="MQREPITTVAISESPVEKFREFLEIRGEKLTEPRRVLVRHIFNS
                     HKHFDADELVRDLHDAGHHVSRSTVYRTLRLLVEAGLLRELRLTNRSAFEHDYGYPTH
                     DHLHCTSCNRIVEFRNDEILKLRDSVSLVHGFRPSGHRFLISGICSACSRSHTPRRRL
                     DLI"
     gene            complement(1225..>1657)
                     /locus_tag="VHS97_24880"
     CDS             complement(1225..>1657)
                     /locus_tag="VHS97_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451511.1"
                     /translation="HHIRPADLRPIVDAVQEGDLYRVRGHCGAPSTDFIEYVHVVIYE
                     RRLGRLSAHSFSYSGSTQGCDQSVRQLHTILENLQAKLSRQSNPSQTGPSPTVTPDGS
                     NPLPGGPAPAFGPHIGNQPTAPAVNPGGGSVIPLTPVDRLQ"
BASE COUNT          341 a          501 c          504 g          311 t
ORIGIN      
        1 cccaggcaga tcgacgacgc tcgctccgcc gccgccatgg cactcgccga ggccaagcat
       61 ccccaacacg gctcgccact gtttccacaa atcatcgaga ccgccgctgc ttaccagatc
      121 gatccgaccc gggaaggata tccggacttg atcgcgctgc cggatgaacc gtactgggtc
      181 cgcacgaaac tgacatcgag tgtgtcgtgg gtcgagccag accccaatct gcccggaact
      241 caccgtcccg aaggaatcgt cgcgctgggc gcggctggac tggctccagg ccgttatttg
      301 aacgcccatc tgattgacgc cacgccgaca atcctcgctc ttctggggca agccatcccg
      361 gcgcatatcg aaggcaagcc gatcgtcggc acggctgcga caccccacga aactccctgc
      421 ttcgccccaa ggcggcacga tggcgcggaa caactgctcg aaggacctca taagaagccg
      481 ttcgagtatt cctccgagga gcaggaaatc atcgaacagc gcctcgccga tctgggctat
      541 ctggagtgat tgtgcccacc cagactcgct cgcaaatctc tttaaatcaa atccaaccga
      601 cgtcgtggcg tgtgcgatcg gctacaggcc gagcagattc ccgagatcaa gaagcggtgg
      661 cccgacgggc gaaagccgtg caccagactg accgaatcgc gcagcttgag gatctcgtca
      721 ttgcggaatt cgacaatccg gttgcatgag gtgcaatgta ggtgatcatg ggtcgggtaa
      781 ccgtagtcgt gctcgaacgc gctgcgattg gtcaaccgga gctcgcggag aagtcccgcc
      841 tcgaccagca agcgcaatgt gcggtagacg gttgagcggc tgacgtgatg accggcgtcg
      901 tgcaggtcac ggacgagctc atcagcatcg aagtgcttgt gcgaattgaa aatgtggcgg
      961 acgagcacgc gccggggctc cgtgagcttt tcccctcgta tttccaggaa ctcgcggaat
     1021 ttctcgaccg gcgactcgga aatcgccaca gttgtgattg gttcgcgttg catttccgcc
     1081 ccgattccga ttcgcgccgc tccacgatcc aggtgacacc attcacgcgg catggtttga
     1141 atcaataatg attctccatt ctaccgacgg ttacggcagt cttccagtcg acgcgatgcg
     1201 aaggcgcacg acagatcgag gtcttcactg caaacgatcc accggtgtca acggaatcac
     1261 tgaaccgccg ccggggttga ccgccggtgc ggtgggctgg ttgccgatgt gagggccaaa
     1321 cgccggtgcc ggaccgcccg gaaggggatt tgatccatcc ggagtgacgg tcggtgacgg
     1381 gccggtctga ctcggatttg actggcggct caacttggcc tggaggtttt ccaggatcgt
     1441 gtgcagttga cgaaccgatt ggtcgcaccc ctgtgtactg cccgaatacg agaacgagtg
     1501 agcagacaag cgtcccaatc ggcgttcgta aatgacgacg tggacgtatt cgatgaaatc
     1561 cgtcgaaggc gcgccgcagt ggccccggac ccggtagagg tcgccctctt gaaccgcgtc
     1621 gacgatcggc cggagatcgg cggggcgtat gtggtgg
//
LOCUS       DATAQL010001441         9509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5340592, whole genome shotgun sequence.
ACCESSION   DATAQL010001441 DATAQL010000000
VERSION     DATAQL010001441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5340592"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..1341
                     /locus_tag="VHS97_24885"
     CDS             550..1341
                     /locus_tag="VHS97_24885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451512.1"
                     /translation="MNKFTYAIALGAGLVLSLTAPKVQADPIVQTLTPATISAAQFNT
                     DFTPVTTVLSQPYAFINTNSAGVVESQVFQGKGALAGLYAYAYQFGVNNVNDTSTNQP
                     TSVNSASMLFNATPLTTNQIVPGTTSATYVVTDGQVGQINVPQAAPGTVVQTPASIAW
                     LPGTSTGSLTFQYLNPTTNSSPLGAGATSGTVVVISNQPHTQQFVSVQNPEPQTTYPK
                     AFSPSGGPIEQVPAPEPATVLAWTSVIAALAVGHRFRRAHKVIVA"
     gene            1669..2448
                     /locus_tag="VHS97_24890"
     CDS             1669..2448
                     /locus_tag="VHS97_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HEX3451513.1"
                     /translation="MNPEREPAGVKCNTPRPEHSEREPTAAALDAKGATGRSNIVPYM
                     APMIAYIALGALESYLPSNDGQPSPVWYPLAYTARVVLVALVAWRYRATWSDFRPWPS
                     PAVTGLAVLIGVIVWGLWIGLDGHYPALPFLGTRVAFDLGVMSPAARWAFVGARMVGL
                     VVVVPLIEELFWRSFLIRWLIDQEFERVPIGRITPLAAVVTSVSFALVHPEWLPALLT
                     GALWVWLLWSTRSLGACLVSHATANLALGLYVIAAGDWKYW"
     gene            2455..3696
                     /locus_tag="VHS97_24895"
     CDS             2455..3696
                     /locus_tag="VHS97_24895"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3451514.1"
                     /translation="MIIPWGTDAPLYHRPIATVGLIVVNIVVYIMFPTALNQDWTLAL
                     GDGIHPAQWVTNLFLHSGFWHVAGNMIFLWTFGFVVEGKLGWWAFMLVYLGLGTVESA
                     GMQLIVNTAEPTHLMGSSGIVFGLLAMCLVWAPKNEVVCILWLRFAPTVFDAPIVLFA
                     VGYIVLDVLTSSLTGVLMANVTNRSTGAILAVVLDHSVGAVLGFVLAVALLKLKWVDC
                     ENWDLFAVMEGRQGQSKAAARRAKARVHLVSAEYRRSNTPKQKKKGRKAKGTVSSIED
                     AAAVALRTLRRHLELDEVEAAVAVYQKSSRSLAGWQPEESDWIALIQAVLDQNAWGDA
                     ALIMRDYVKRSPAPSPRIRMKFAQVLIQRLSRPTLGLTVLDQIPQGVLPESLEATRRK
                     LVRQAEQMREDGELELQDELW"
     gene            complement(3726..4823)
                     /locus_tag="VHS97_24900"
     CDS             complement(3726..4823)
                     /locus_tag="VHS97_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451515.1"
                     /translation="MEKKRLSPVPSPSRRIVSLDQFRGYTVVGMLFVNFLGGYQVVPP
                     IFKHHNTYCSYADTIMPQFFFAVGFAYRLTFLRRLAAAGVLAAAAAVLKRNAGLLLLG
                     FVLYHLDGKVQSWAELEQLGLHGFLTQAFRREIFQTLVHIAITSVWILPVIGAGPAIR
                     ILFMIASAGLHLWLSNRFYFHWAWTVPVIDGGPLGFMTWAIPTIVGSLAYDAVAQSHD
                     DPNLIAKLTGWSFVFLTLGYALSCMGGYRATPPFLPPSASQVVDIWTMSQRTGSVSYL
                     TFSAGVSLAVYALFVLICDHGGWQFSLFRIFGQNALAAYIIHPMVASAVKPYLPKNAP
                     LWYVAAGFGLYFAICALLNRYLEKHELFLRL"
     gene            complement(4835..5842)
                     /locus_tag="VHS97_24905"
     CDS             complement(4835..5842)
                     /locus_tag="VHS97_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3451516.1"
                     /translation="MRTRIGFDHYTIAHRGFTARQTLEFAQAHHFDGVQFLEPTSIDP
                     RLDYARLSEFRRQADAMGLYLEIGLPSPNPAHRSRELDREVAPAELAQELLPHVEAVA
                     ALSCRHARVFVGNRHDRFRADNPWSSQIEATIEVIDRLTPALKRLGIRLAIETHADLT
                     GDELIALLGRIDPDIAGVTLDTGNIVMRLDDPVELARRLAPYVVATHVKDAVLAFTPR
                     GLCWQARPVGSGIMPLPDILAVLLRQNPAVTLSIELHPRTYDLPIHDDKWLSYFPGLR
                     PLSLAAVVRLAALAEERYLDGSLEPPETIEAVPWPCRDLDWLASSLGYLRSVVPTLAA
                     I"
     gene            complement(6935..7462)
                     /locus_tag="VHS97_24910"
     CDS             complement(6935..7462)
                     /locus_tag="VHS97_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEX3451517.1"
                     /translation="MRTQRIAMGLVVATGLWQSGCATTAPSPERRLTADNSYSAGRAI
                     QDFALPANKVGEALTAAMDDLKMTAIEPGRDGAVYKIDAKTADNRTVMVTMRPRQGQT
                     RLSCRIGWFGDEALSKALLERTGVRLGTLPPAAVPEDPPSTPGSNPFFSRNAVPDEEM
                     LRDVAEAPYRDRVIP"
     gene            7843..8355
                     /locus_tag="VHS97_24915"
     CDS             7843..8355
                     /locus_tag="VHS97_24915"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3451518.1"
                     /translation="MKIPTIAKLLEDQLKDIHSAEAQLVKALPKMAKKASSSELKNAI
                     TSHLEETRHHVERLDQIGQTLGVKLSGKKCKAMEGLIQEGAEVLEADGPGSVIDAALI
                     AAAQRVEHYEISAYGSARALASHLAHDDIVELLQETLDEESAADEKLSSISEEEILPT
                     ALAVEQGVGG"
     gene            complement(8352..9206)
                     /locus_tag="VHS97_24920"
     CDS             complement(8352..9206)
                     /locus_tag="VHS97_24920"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX3451519.1"
                     /translation="MPGVRNRRSSRRSALAVIIAVFFILNQAPPASAWGRLGHRVVAQ
                     LAELHLTSKTREAVKILLEPGETLADCSTWADQHQREVKGSGCWHYVDVPLNEPRYDA
                     RFSAPVPEKGCIVDKIAEFRDILKDQSRTIQERRLALRFVVHLVEDLHMPLHVGDNDD
                     RGGNDTQVRWFEFGSNMHRVWDTQMIERTANTEDAWLNTLRELDSESNRLKWMSGTVE
                     DWATESLLLARAAYLIPGTGAPIKSGQELGDSYRAANLPSVRQRLAQAGVRLAWVLNQ
                     AFPENGLR"
     gene            9240..>9509
                     /locus_tag="VHS97_24925"
     CDS             9240..>9509
                     /locus_tag="VHS97_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451520.1"
                     /translation="MTTVSQSIIITEIAKVNPTGAAPQIALAGCFAAKGVDMRIKQLK
                     WICAVLAALGVGTKIDAHAQDTTEFRVGAADTDITPPQGIPMWGYG"
BASE COUNT         1937 a         2805 c         2937 g         1830 t
ORIGIN      
        1 gggctatcgc caccgagcga cgggagtggc aagatgctct cgggtttctc aagcgcagtc
       61 tggcgcgctc ggcgccgagc gattcgattg tgcggaaact gtatgccttt agcctggtgt
      121 gtcttcacat tctttagcgc gatcgcggcc gaaatcgtgt tgacctgttt ttcggttcct
      181 gatagggttc cgatcgcctt ggtgaggttg gcgttggatc caaggcacga aggtgagaga
      241 gtcgcgtccc tcgcgaccgc gactggtcga atgtcgacgg caccctggcc caagccatga
      301 agcagggcga ggcgtctccg atccggcgat cggtatatct aaggaatgac aactcgacac
      361 ggaagtatga agaaccgggg cgtgtccggt gattttcttc aagacttcca cgcaccccaa
      421 actcgacgcg accaaggccc gcgaggcccg tccggagacg ttctccggcc ggcgagttat
      481 ggaatgactc gctcccctgg gttcgttcgc gtgacgaacc tttccgatca accctggaag
      541 atcgctagaa tgaacaagtt cacttacgca atcgccctgg gggctggtct ggtgctgagc
      601 ctgaccgccc cgaaggttca ggccgacccg atagtccaga cactcacgcc ggccacgatc
      661 tcggcggcgc agttcaatac cgatttcacc cccgtcacga ccgtcttgag ccaaccatac
      721 gcgtttatca acacgaacag cgcgggtgtc gtggaatctc aggtgtttca gggcaagggc
      781 gccctcgccg gtttgtatgc ctacgcctat cagttcggcg ttaacaacgt taacgataca
      841 tcgaccaatc aaccgaccag cgtcaacagc gcgtcgatgc tgttcaacgc gacgccgctc
      901 acaaccaatc aaatcgtccc gggaaccacc tcggccacct atgtggtgac tgacggtcaa
      961 gttggccaga tcaacgtacc gcaggctgca cccggcacgg tcgtccaaac gccggcttcg
     1021 attgcctggc tgcctggcac gtcgaccggg tcgctcacct ttcagtactt gaacccgacc
     1081 accaacagct cccccctcgg agccggcgcg accagtggca ccgtggtcgt gatctcgaat
     1141 caaccgcaca cacagcaatt cgtgagcgtg caaaaccccg agccgcagac gacttatccc
     1201 aaggctttct ctccctcggg aggtccgatc gagcaggtgc ctgcaccgga acccgcgacc
     1261 gtcttggcgt ggacgagcgt catcgccgcc ctcgcggtcg gacaccgctt tcgccgtgcc
     1321 cacaaggtta tcgtcgcttg aatagagtgc gacgtcaggc gcggcgaatc cttcacaagc
     1381 ggcgagtcgc ctacgccgct ttccgtccta atcaaaacga ttccgcccct tgcggacgcc
     1441 tgagatggcc gcaaggggct ttgtcgtttg gcttctggcc gggtaaaatc tcgcctgcgt
     1501 caattgtcgt gcaattgccc gtaacgtacg cgaattgaag cgacagcgat ggcggcacgg
     1561 gcgcggaggc ccggaagttg attcgtgcgg tcggatttga tcgaccgtgg agacgaaccg
     1621 gcgctctgga gcttgatcga atccgcgtcc tagggaaatc aaacccggat gaaccctgaa
     1681 cgagagcccg ccggcgtgaa gtgcaacacg ccgcggccgg aacactctga gcgagagcca
     1741 accgctgctg cgttggacgc aaaaggggcg acgggccgct ccaacatcgt gccgtacatg
     1801 gcacccatga tcgcctacat cgcgctaggc gcactcgagt catatctgcc ttctaacgac
     1861 ggtcaaccaa gccccgtctg gtatccgctg gcgtacactg cccgcgtcgt gttagttgcg
     1921 ctcgtagcat ggcgatatcg ggccacgtgg agcgacttcc ggccgtggcc aagccccgct
     1981 gtgaccgggt tggcggtatt gatcggagtg atcgtctggg gcttgtggat cggcctggat
     2041 ggtcactatc cggccctgcc gtttctcggc acgcgggtcg cctttgatct cggcgtcatg
     2101 tcgccggcgg cgcgctgggc gttcgtcgga gcgagaatgg tgggattggt cgttgtcgtg
     2161 ccgttgatcg aagagctatt ctggcgctct ttcttgattc gttggctgat cgaccaggaa
     2221 ttcgaaaggg tgccgatcgg gcgaatcacc ccgttggcgg cggtcgtcac ctcggtctcg
     2281 tttgcgctcg tacatccgga atggctgccg gcgctattga cgggggcgct ctgggtctgg
     2341 ctgctatggt cgactcgatc actcggtgcg tgcctggtga gccacgccac ggctaacctg
     2401 gcgctgggac tttacgtgat tgccgccggc gattggaaat actggtagat acgaatgatc
     2461 atcccatggg ggactgatgc gccgctctat caccgaccga tcgccacggt cggactgatc
     2521 gtcgtcaaca ttgtcgtcta catcatgttc cccaccgccc tcaaccagga ctggactctc
     2581 gccctgggag atgggattca ccccgcacag tgggtgacga acctcttctt gcattcggga
     2641 ttctggcacg tcgccggcaa catgatcttt ctctggacgt tcggctttgt cgtcgaggga
     2701 aagctgggct ggtgggcgtt catgctggtc tatctcggtt tgggcacggt cgaaagcgcc
     2761 ggcatgcagc tcatcgtgaa taccgcggag ccgacgcacc tgatgggatc atcgggcatc
     2821 gtatttggcc tgctggcgat gtgcttggta tgggcaccca agaacgaagt tgtatgtatc
     2881 ctctggctgc gatttgcgcc gacggtattc gacgcaccga ttgtcttgtt cgccgttggt
     2941 tacatcgtgc tcgacgtgtt gacttcgagt ttgaccgggg tcttgatggc gaacgtaacc
     3001 aaccgctcca ccggcgcgat tctcgccgta gtgctggacc actcggtggg cgccgtgctc
     3061 ggattcgtgc tggcggtcgc gctgctcaaa ctcaagtggg ttgattgtga aaactgggat
     3121 ctgttcgccg tcatggaagg gcggcagggc cagtcgaagg cggcggcccg gagagcgaag
     3181 gcacgcgtcc atctggtgtc tgccgaatat cgtcggtcga acacacccaa acagaagaag
     3241 aagggcagga aggccaaggg gaccgtgagc tcgatcgaag atgccgcggc tgtcgcgctg
     3301 cggaccttgc gccggcatct ggagctggac gaagtcgagg cggccgttgc agtctaccaa
     3361 aagtcgagcc gttcgctcgc aggatggcag ccggaagagt ctgactggat cgccttgatc
     3421 caggcagttc tcgatcaaaa cgcctggggc gacgcagcat tgatcatgcg tgactacgtg
     3481 aagcgctcgc ccgcgccgtc accccgcatc cgcatgaagt tcgcccaggt cctgattcag
     3541 agactgtcgc gtcccacact gggtttgacc gtcctggacc aaatccccca gggtgtgctt
     3601 cctgaatcgc tcgaagcgac acgacgcaaa ctcgttcgac aggctgagca gatgcgcgaa
     3661 gacggcgaac tcgagctaca ggacgagttg tggtaggtcc accggcgaat ctcgttgctt
     3721 acggctcaga gtcgcagaaa gagctcgtgc ttttccaggt agcgattcaa gagcgcgcag
     3781 attgcgaaat agaggccaaa gccggctgcg acgtaccaca ggggtgcatt tttaggtagg
     3841 tacggcttga cggcgctcgc gaccatgggg tggatgatgt aagcggcgag agcattctga
     3901 ccgaagattc gaaacagact gaactgccat cccccgtgat cgcagatgag tacaaatagc
     3961 gcatagaccg ctagcgaaac gccggcgcta aaggtgaggt aggatacgct tcctgtgcgc
     4021 tggctcatcg tccagatgtc gacgacttgg ctggcagagg ggggcaggaa cggaggggtg
     4081 gcgcgatagc cgcccatgca cgacagagca tagccgagcg tcaagaagac aaaggaccag
     4141 ccggtaagtt tggcgatcaa attggggtcg tcgtggctct gggccacggc atcataagcc
     4201 agcgagccga cgatggtcgg gatggcccac gtcatgaagc cgagcggtcc gccgtcgatc
     4261 acggggacgg tccaggccca atggaaatag aatcggttcg aaagccacag atgcaggccc
     4321 gccgaggcga tcatgaaaag gattcgaatc gccggcccgg ccccgatcac gggaagaatc
     4381 cagactgacg tgatcgcgat atgaaccaag gtctggaaga tttcacgccg aaatgcctgc
     4441 gtcagaaatc cgtgtaggcc aagctgttcg agctcggccc acgactgcac tttgccgtcc
     4501 aaatggtaca gcacgaagcc cagtagtaac agcccggcgt tgcgcttgag caccgcggcg
     4561 gcggccgcca aaacgcccgc cgcggcgagc cggcgcagga aagtcaagcg ataggcaaac
     4621 cccaccgcga aaaaaaattg tggcatgatc gtatcggcat aactgcaata agtgttatgg
     4681 tgtttgaaaa ttggcggcac cacctggtag cccccgagga agttgacgaa gagcatgccc
     4741 accacggtgt agccgcgaaa ctggtcgagc gagactatcc gtcggctcgg ggagggcacc
     4801 gggctcaatc gtttcttttc catgcgatgt caattcagat cgccgccaag gtcgggacaa
     4861 ctgaccgcag atagcccagt gagctcgcca gccaatcaag gtcacgacag ggccagggga
     4921 ctgcctcgat ggtttcgggc ggctcgagtg agccatccaa ataccgctct tcggccaacg
     4981 cggccaggcg gacgaccgca gccaacgaaa ggggacgcag gccaggaaaa taggacagcc
     5041 acttgtcatc gtggatcggc aggtcatacg tccgcggatg tagctctatc gaaagcgtga
     5101 ctgccggatt ctgacgtaag agcacggcca ggatgtcagg caggggcata attcccgagc
     5161 ccaccggacg ggcctgccag cacagcccgc gcggggtgaa cgccaggacc gcgtctttga
     5221 cgtgcgtggc cacgacatag ggggccagcc gccgggccaa ctcgaccgga tcatcgagcc
     5281 tcatcacgat gttgccggta tcaagggtca ccccggcaat gtcgggatcg atccggccca
     5341 agagcgcgat caactcatct ccggtcaaat cggcgtgggt ctcgatcgcc agcctgattc
     5401 ccaggcgttt gagcgccggt gtgagccggt caataacctc gatggtcgct tcgatctggc
     5461 tcgaccacgg gttgtcggca cggaaacgat cgtgccgatt tcccacaaac acacgggcat
     5521 gacggcacga aagcgcggcg accgcctcga catgcggcag gagctcttgg gccagctcgg
     5581 ccggcgcgac ctcgcgatcg agctcgcggg aacggtgcgc tgggtttggt gacggcagcc
     5641 cgatctcaag gtatagcccc atcgcgtcgg cttgccggcg aaactcgctg agccgcgcat
     5701 agtccagtcg tgggtcgatt gacgtcggct cgaggaactg cacgccatcg aagtgatgag
     5761 cctgggcaaa ttccaaagtc tgccgtgccg tgaatccacg gtgagcgatc gtgtagtggt
     5821 caaacccgat ccgtgttcgc accagcgatc gctcccagta ttcgtaggcg attggcaccg
     5881 ctctttgccg gttgcctttc ctgcttccgg aacaacccgc cagggcgtgt gccaccgtcg
     5941 gacgtccgga gatcgaagga gtctcgtccg tgggctaatt ttggcatctt ggtcggtatt
     6001 ccgaggcccc gaggagtcag gcagagcgag ccaagccacg agtctgccat ggccagggcg
     6061 gcaacgcaac ggtctacctg cgagtcgaaa gggcaccagg taagggaaac aggtgcggta
     6121 cgcaggtagc cggctggcgc aaggcggatc cgaaataatg tgcggacctg gccggcaatt
     6181 gccgactgga tcctgcggcg cacgggacct cgaacgcaaa aaaatctggc gagagggttg
     6241 acgagtttcc aggatagtcg tattttagcc ttcgtcttca gggtacaaca cattgatgat
     6301 cgcttgggtg atcgctctca cttatgttag tatcgagatt gactcctgcc gggtagtccg
     6361 actttcgtcg ggaaacgggt tcaccgtttt ccgaaaatac gttatcccaa aaaaatgagg
     6421 ttcgattcct ggcggcacgg atgcgactgc caggttcggc ccttctagtg gcgagggatg
     6481 atcgaggttt agaccaagat cacacccctg gcgccgaggc ctcccggtcc gctgttcgat
     6541 tcggcaagga tgcctgaatc gacgcgggcc gcgattggcc aaactaaaca gtcgaaggtg
     6601 ctcgagaacc cgcggagacc tgcggtcggc ggaggggcgg ggtcatgaga cccgcccacg
     6661 acagaagggc gaggtcagcg taaagggagg gggcttttct ggcacctgcc acggaggtca
     6721 taggccccgc tacagcacac tcgcggccga tgcatgtgac tggctccctc cctagggaaa
     6781 gggagctagt gccgacggaa acccctccct ctaacggcgg ggtcaggaag gggcggggtc
     6841 gttcattcgt cgcgggtcta gtcatgagac ccgcgcccaa cgggtcaaga gacccgcgcc
     6901 caacgggtca ggagacccgc gcccaaagtc gacgctaggg gatcacgcga tcgcggtagg
     6961 gcgcctcggc gacgtcgcgc agcatttctt catcagggac ggcattgcgc gagaagaaag
     7021 ggttggaacc gggagtgctc gggggatctt cggggaccgc ggccgggggg agcgttccga
     7081 gccggacgcc cgtgcgctcc agaagagcct tggacagcgc ttcgtcgccg aaccagccga
     7141 tccggcaact gaggcgcgtt tgcccctggc ggggccgcat tgtcaccatg acagtacggt
     7201 tgtccgcggt tttggcgtcg atcttgtaga cagcaccatc gcgccctggc tcgatcgccg
     7261 tcatcttgag gtcgtccatg gccgccgtga gtgcttcgcc aaccttgttg gcaggcagcg
     7321 cgaagtcctg aatggctcgg cccgcactgt aagaattgtc ggcagtgagt cggcgctcgg
     7381 ggctcggtgc cgttgtggcg catccgctct gccagagtcc ggttgccacc accagtccca
     7441 tcgcaatccg ttgcgtcctc atcgtcggcc tcacgtccga acctgatttc aagctacccg
     7501 cgccgcgccg gcctcggccc tgaccgccgc aagttatctc gacggggcaa aatggtcaag
     7561 agcggtcaga tcgtgaataa cgggccgcga ccaataatga aagcgatcac gtcgaccatt
     7621 cacgtgtgtt tcatggcgat aaatgtgcga attgcacagt atttgaaaaa tgttatttat
     7681 gattattatc tgctaccatt caaccgcgtc gttcccaccg tcgaccgaca tatgggaaag
     7741 cacggtcgcg cggcatcgcg tttgcttaac tcgaaatggc gttggcgcca cagagcgaaa
     7801 accgactctg caactagaaa tcaaaataag aagaggacca tcatgaaaat tccgacgatt
     7861 gccaagttgc tcgaagacca gctcaaggat atccacagcg cggaagcgca gctcgtcaag
     7921 gcgcttccca agatggcaaa gaaggcttct tcttccgagc tgaagaacgc gatcacgagc
     7981 catctggagg aaacgcgcca tcatgtcgag cgtctggacc agattggtca gacgctggga
     8041 gtcaagctct ccggcaaaaa gtgcaaggcc atggagggac tgatccagga aggggcggaa
     8101 gtactcgagg ccgacggccc gggctctgta atcgacgccg ccttgatcgc cgcggcccag
     8161 cgcgtggagc attacgagat ttcagcctat ggctcggctc gcgctctcgc cagccacctg
     8221 gcgcacgacg acatcgtcga gcttcttcag gagacgctcg atgaggaatc tgcggcggat
     8281 gagaagctgt ccagcatttc tgaggaagag atcctcccaa cagccttggc tgtcgagcaa
     8341 ggcgtaggag gctagcgcaa tccgttctcc ggaaacgcct ggttgagcac ccacgcgaga
     8401 cgtacacccg cctgggccag acgctggcgg accgacggta gattcgcagc gcggtaagag
     8461 tcgccgagct cttggccgga cttgatcggt gcgcccgttc ccggaatcag ataggccgcg
     8521 cgggccagca gcaaactctc ggtcgcccaa tcctcgactg taccgctcat ccacttgagc
     8581 cgattcgatt cggagtcgag ctcgcgaagc gtgttcaacc aggcgtcctc ggtattggcg
     8641 gtcctctcaa tcatctgcgt gtcccatacg cggtgcatgt tgctgccgaa ttcaaaccac
     8701 cttacttgcg tgtcgttgcc gccccgatcg tcgttgtcgc cgacatgcag cggcatgtgc
     8761 aggtcctcga ccaggtggac gacaaaccgc agcgcgagcc ggcgctcctg aatcgtcctg
     8821 ctctggtctt tcagaatgtc gcggaactcc gcgatcttgt cgacgataca tcctttctcg
     8881 ggaaccggtg ccgagaagcg cgcatcgtat cgaggctcgt tcaaaggcac atccacgtaa
     8941 tgccagcagc cgctcccctt gacctcgcgc tggtgctgat cggcccatgt cgagcaatcg
     9001 gcaagtgtct ctccgggctc cagcaagatc tttactgcct ctcgtgtctt cgaggtcaaa
     9061 tgcagctcgg caagctgtgc gacaacccga tgcccgagcc gcccccacgc cgaggccggc
     9121 ggtgcctggt tcaggatgaa aaacacggcg attattaccg cgagagccga acggcgagat
     9181 gatcgtctgt ttcgcacacc gggcataaac ttctccgagt aagctgctga gtcgctcttt
     9241 tgaccacggt ttcgcaatcg attatcatca ccgaaattgc taaggtcaat ccgaccggag
     9301 ccgctccgca aatcgcgctg gcgggatgtt tcgccgcgaa aggtgttgat atgcgcataa
     9361 aacagttaaa gtggatctgc gccgttttgg cagcgctggg cgtgggcact aaaattgatg
     9421 ctcatgccca ggacacgacg gaattccgtg taggcgcggc ggacacggac attacgcccc
     9481 cacagggaat tccgatgtgg gggtatggt
//
LOCUS       DATAQL010001442         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4117857, whole genome shotgun sequence.
ACCESSION   DATAQL010001442 DATAQL010000000
VERSION     DATAQL010001442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4117857"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VHS97_24930"
     CDS             complement(<1..730)
                     /locus_tag="VHS97_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX3451521.1"
                     /translation="MNRPGRSCVRMIVLGAVVVIFTTGSPASAQRAPFPIPDPDPEIE
                     RKSFVVADGFQVNLFASDPLIAKPLQMNFDPMGRLWIASSEVYPQIKPGAVANDRVLV
                     VEDRDHDGRADKTTIFAQGLLIPTGIEPGDGGVYVANSTELLHFKDSDGDGKADTTRV
                     VLSGFGTEDTHHILHTLRWGYDGMLYFNQSVYIHSHIETPRGVRRLGGGGIWQFRPET
                     MELEVFTHGLVNPWGHHFDRWGQSF"
     gene            complement(727..1983)
                     /locus_tag="VHS97_24935"
     CDS             complement(727..1983)
                     /locus_tag="VHS97_24935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3451522.1"
                     /translation="MRVLFSGLIGLLCLSASTEARSDQPPHFELRNGDRVVFLGDTLI
                     ERDQRYGYLETLLTIANPDKDLVFRNLGWSGDTVGGVSRAGFGRPEAGFEQLRQQLAA
                     VKPTMVLVGYGMADSFAGEMGLPGFEQGLNRLLNVIDSMKARVVLLSPVAHADLGRPL
                     PDPAVHNRALKYCSDVIHKVAVDRKSGFVDLFDFFENRYARGREYRLSLTTDGIHLSA
                     AGYHAAASRIGELFGYNHALPGCLVRLGHDGKVIVQERARPSQVATTPRGLRFELIDD
                     LLPMPSFTESRTSGGENGSARIVSIQGLMPGKYELKIDGTGVATADAAGWHQGVALWR
                     SPEFQQADLLRAAINRKNELFFHRWRPQNNTYLFGFRKHEQGNNAVEIPQFDPLVAEQ
                     EKIIARLKKPIAHVYELSRMEREVGL"
     gene            2192..2908
                     /locus_tag="VHS97_24940"
     CDS             2192..2908
                     /locus_tag="VHS97_24940"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3451523.1"
                     /translation="MSTITSPPPSVATVPPLPVPSAAIHAEQRIAIRDIPWDLYDRLS
                     EAIGEGQNVHLAYDGKDLEIMVIGPTHEDAKEGLHLFVKAVAFELRIRCRGLGQTTWK
                     RPETLRGIEADLCYFFDPAKLAIAADARMRRSNDIADYPNPDLAIEIDISPSQVDRPG
                     IYRALSVKEVWHFDGSSLVIDQLGSDGTYVASESSLFLPVRADEVFRWVAEEDSNDEI
                     AWETRLREWARSELAPRMKP"
BASE COUNT          629 a          930 c          897 g          592 t
ORIGIN      
        1 cgaatgattg gccccagcgg tcgaaatgat ggccccacgg gttgaccaga ccgtgggtga
       61 acacttcgag ctccattgtc tcgggccgaa actgccagat cccccctccc cctagccggc
      121 gaactccgcg aggtgtttct atgtggctgt gaatgtaaac cgactgattg aaataaagca
      181 tcccgtcata cccccacctc agcgtatgga ggatatggtg ggtgtcttcg gtaccaaacc
      241 ccgagagaac aacgcgggtc gtgtcggcct tgccgtcgcc gtcggagtct ttgaagtgca
      301 acagctcggt actgttggcc acgtaaacgc cgccatcgcc gggctcgatt cccgtcggga
      361 tcaacagccc ctgggcaaag atcgtcgtct tgtcggctcg gccgtcgtga tcgcgatcct
      421 caacgacaag caccctgtcg ttggcgaccg cgcctggttt gatctgagga taaacctccg
      481 agctcgcaat ccagagccgg cccatgggat cgaagttcat ttggagcggc ttggcaatca
      541 aggggtcgga cgcaaacaga ttgacttgga agccatcggc gaccacgaac gatttccgtt
      601 cgatctcagg atcgggatcg gggatcggga agggtgcccg ctgggccgag gctggcgaac
      661 cggtcgtgaa gatcacgacc accgcgccga ggacaatcat gcgaacacac gaacgacccg
      721 gtcgattcac agtccaacct ccctctccat ccgcgacaat tcataaacat gagcaatcgg
      781 ctttttgagc cgcgcgatga tcttttcctg ctcggcgacc agcggatcga attgggggat
      841 ctcgacggcg ttgttgcctt gctcgtgctt gcggaaccca aagagatacg tgttgttctg
      901 cggccgccac cgatggaaga acagctcgtt cttgcgattg atcgcggccc gcagtaagtc
      961 ggcctgctga aactcggggc tgcgccagag cgccacgccc tgatgccatc ccgcggcatc
     1021 ggcggtcgcc acgccggtcc cgtcgatctt caattcatac tttcccggca tgagtccctg
     1081 gatcgataca attcgggcgg acccgttttc tccgccggac gtacgtgact cggtgaatga
     1141 gggcataggc aagaggtcgt cgatcagctc aaatcgcaaa ccccggggcg tggtcgcaac
     1201 ttgagatggg cgagcccgct cctggacaat gaccttgccg tcatgtccga gcctgactag
     1261 acagcccggc agcgcgtgat tgtagccgaa taattcgccg atcctcgagg ctgcggcgtg
     1321 gtagcccgcc gcgctcaagt gaatgccatc ggtggtgagc gatagccgat attcgcgccc
     1381 gcgggcatac cgattttcga agaagtcaaa cagatcgaca aaaccgctct tccgatcgac
     1441 ggccaccttg tgaatcacgt cgctgcagta cttgagcgcg cggttgtgga cggctgggtc
     1501 aggaaggggc cgaccaaggt ccgcatgggc aaccggcgac agcagaacga ctcgcgcctt
     1561 catggagtcg atgacgttca gcagcctatt caaaccctgt tcgaacccgg gaaggcccat
     1621 ctcacccgcg aacgaatcgg ccatgccgta gccgaccaaa accatcgtcg gcttgaccgc
     1681 cgcgagctgc tgccggagct gctcaaaccc agcttccggc cggccgaatc ccgctcgaga
     1741 gacaccgccg accgtatcgc cgctccagcc aaggttgcga aagaccaggt ccttgtcggg
     1801 attggcgatc gtcaggagcg tctccaagta accataccgc tggtcgcgtt cgatcaatgt
     1861 gtctcccaga aatacgacgc ggtcgccgtt gcgcagctcg aaatgaggcg gttgatcgct
     1921 tcgggcctcg gttgacgcgg agagacacag cagcccgata agacctgaga aaagaactct
     1981 caccgaaaag gctcccagac gcggacgtcg cgacacggat tcaggcatga tcgaacaggc
     2041 gggccggtag ctgcgggatc acacgacggc tttgattgta agccgcggtc cagccacggt
     2101 cgagaccggt accgaatgga tggagaggtt tcagtctggt cggctataat acgatgtcaa
     2161 acgatgcccg agcccgacag gagtcgactt catgtccacg atcaccagcc cgccgccgtc
     2221 tgtggcgaca gtgcccccct tgcccgtgcc gtccgccgcc attcacgcag aacaacgaat
     2281 cgcgatccga gacattccct gggatcttta cgaccggcta tccgaagcga ttggcgaggg
     2341 gcagaatgtg catctggcct atgacgggaa ggatctggag atcatggtaa ttggcccgac
     2401 ccatgaggat gccaaggaag ggttgcattt gttcgtcaag gcggtagcct ttgagctgag
     2461 aattcgttgt cgcgggctcg gccagaccac ctggaaacgg ccggaaacgc tccgaggcat
     2521 cgaggccgac ctttgctatt tcttcgaccc ggcaaaattg gccatcgccg cggacgcacg
     2581 gatgcgccga tccaacgaca tcgctgacta tcccaatccc gacctggcga tcgagatcga
     2641 catctccccg tcccaggtcg accggcctgg catctatcgt gcgcttagtg tcaaggaggt
     2701 ctggcatttt gacggcagtt cgttggtgat cgatcagctc ggcagcgatg gcacctacgt
     2761 cgcgtctgag tcaagcttgt ttcttcccgt ccgggctgat gaggtttttc gatgggtcgc
     2821 cgaagaggat tccaatgacg agatcgcctg ggagacccgc ttgcgggaat gggctcgttc
     2881 cgagctggcg ccgcggatga aaccataggc ctgaaccaaa cagccgcgct cgatcatact
     2941 gaggcggccg tctcttgcgt taccatgtcc cgtcacgttt tcaccaagtt ctccttgctc
     3001 acctccgcga acgacttgca cccgctcagg cccagcgtca agtcgatg
//
LOCUS       DATAQL010001443         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2746395, whole genome shotgun sequence.
ACCESSION   DATAQL010001443 DATAQL010000000
VERSION     DATAQL010001443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746395"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VHS97_24945"
     CDS             complement(<1..1155)
                     /locus_tag="VHS97_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX3451524.1"
                     /translation="MAKTRDWTEILVRRGVIGPDQLKEANRMESVAVEEALVKLGYAD
                     ADDIMKAKAEQHGLPFVELKEIEIPASVIELVPESLARENIVMPLSQQSGVIRVIMHD
                     PLDFETIDKLRFVLNREIEVSLAPKESIVEAINKYYGSATSETESVDSMLQEFTDTAI
                     DFSEDGGVGGKSSVTNTLEEGDAPVIKLVHLIIQEAVTMRASDIHIEPFAERVRIRYR
                     IDGVLMERDNAPRRLLGAIISRIKIMGFIDIAEKRRPQDGRIKILVAGKDIDMRVSII
                     PTSHGQSVVMRILDRENIKVGLQDLGFGDEDFGRFKQLIKRPNGILLVTGPTGSGKTT
                     TLYAALNELNRPDTKIITAEDPVEYYLPGVNQCEVKAKIGMTFARIIRAML"
     gene            complement(1456..2523)
                     /gene="purM"
                     /locus_tag="VHS97_24950"
     CDS             complement(1456..2523)
                     /gene="purM"
                     /locus_tag="VHS97_24950"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX3451525.1"
                     /translation="MNEPLDYRSTGVDLKVYEETMSRLPPLMRRTFSPRVIEWKDGFA
                     GLFRLDDQIGLLSRTYRDPVLVASTDGVGSKLKVALATGRHATVGIDLVAMSVNDCLC
                     AGAEPLLFLDYVAMNRDDPELTTQIVKGISDGCIEAECALLGGETAILPDIYKEGDYD
                     LAGFCVGVVERKHILDGRDIRAGDRVIGLASSGLHSNGYSLVRKIVFERGGLLPETVV
                     KELGRTVADILLEPTRIYVRAVKTVYRHYRVKRIVHGMAHITGGGLIDNPPRVLPEGC
                     SIRLRRGSWQIPPVFPWLQRLGDVDRAEMDHVFNMGIGLVLIVAEYYADAIVRHLTHQ
                     VKVPAWIIGEVVPGERKVEWD"
     gene            2891..3358
                     /locus_tag="VHS97_24955"
     CDS             2891..3358
                     /locus_tag="VHS97_24955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451526.1"
                     /translation="MIRKRLAFLLGAGFLVVLGIAASSSLSQAQDKEESPLEHIMEKV
                     NKHNTTITKGTRNQVNFAKSQKDVEKSAKELVKLAKSAKPIKDAIKKAKELPEAQKKW
                     DLYIDDLIMTSEKLGEVSAKPSATVADAKSAFNSVKKACADCHKDFRVDEPSN"
     gene            3459..>3649
                     /locus_tag="VHS97_24960"
     CDS             3459..>3649
                     /locus_tag="VHS97_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX3451527.1"
                     /translation="MTCLDLTCSSIAENLALDEALLIEADAGRGDRILRFWEPADFAV
                     VLGASRRIDVDVRLGACRA"
BASE COUNT          766 a         1050 c         1108 g          725 t
ORIGIN      
        1 gagcatggcc cgaatgatgc gcgcgaaggt catgccgatc ttcgccttga cctcacattg
       61 gttcactccc ggcaagtaat actccaccgg atcctcggcc gtgatgatct tggtatcggg
      121 ccggttcagc tcgttgagtg cggcgtagag cgtggtcgtt ttgcccgaac ccgtcggtcc
      181 ggtgacgagc aagattccat tgggacgttt gatcagctgc ttgaagcggc caaagtcttc
      241 gtcgccgaat cccagatcct gcaatccgac cttgatgttc tctcggtcga gaattcgcat
      301 cacaaccgac tgaccatgac tggttggtat gatgctgacg cgcatgtcaa tgtctttgcc
      361 ggcgaccagg attttaatcc ggccgtcttg cggacggcgc ttctcggcga tgtcgatgaa
      421 gcccatgatc ttgatacggc tgatgatcgc gcccaggaga cgccgtggag cgttgtcgcg
      481 ctccatcaag accccgtcga tccgatagcg gatgcgcacg cgctcggcga acggctcgat
      541 atggatgtcg ctcgcacgca tggtgacggc ttcctgaatg atcaggtgca ccagtttgat
      601 aactggcgcg tccccttcct ccagggtgtt tgtcaccgac gatttgcccc ccaccccgcc
      661 gtcttcggag aaatcgatgg cggtgtcggt gaactcctga agcatcgagt cgactgactc
      721 ggtctcggaa gtcgcgctgc catagtattt attgatggcc tcgacgatcg attccttcgg
      781 agccagcgag acctcgatct cgcgattcaa tacaaaccgc aatttgtcga tcgtctcgaa
      841 atcgagcggg tcatgcatga tgacacgaat gaccccgctc tgttgtgaga ggggcatgac
      901 gatgttttcg cgggccagcg actcgggcac cagttcgatc accgatgccg gaatctcgat
      961 ctctttaagc tcgacgaagg gaagaccgtg ttgttcggcc ttggccttca tgatgtcgtc
     1021 ggcgtcggca taaccgagct tgacgagagc ctcctcgacg gcgacgctct ccatccggtt
     1081 tgcttctttg agctgatcgg ggccgatgac accccgccgg acgagaattt cggtccaatc
     1141 ccgagtcttg gccatgactt agcaaactcc agagatgtca attcacgtga gatggcggtt
     1201 ggagaacggg cgatcgccac gggcgctaac ggcgcgcaac cgcgctactc ggattccccg
     1261 gttgtgaagg gcggcgaggc ggattgcggg ataacgtcaa ctacgcggcg cgaccgatcc
     1321 tgtgactgtg ccggaactat ccgtccgggc ttgcccgacg atgtcgactc ggccaattga
     1381 tgggaataag caataggatg atgacacttg aaagtaccag gattagagcg cctcggcaag
     1441 gaactcgtga agaattcagt cccattcgac ttttcgttca cccggcacga cctctccaat
     1501 gatccatgcg ggaaccttca cctgatgggt gagatgacga acgatcgcgt cggcgtaata
     1561 ctcggccacg atcaggacca gaccgatccc catgttgaag acatggtcca tctcggcccg
     1621 gtcgacgtcg cccaagcgct ggagccaggg gaacacgggg gggatttgcc acgagccgcg
     1681 acgaaggcga atcgagcagc cttcgggaag cacgcgcggc gggttgtcga tcaagccgcc
     1741 cccggtgata tgggccatcc cgtgcacgat ccgcttgact cgataatggc ggtagaccgt
     1801 cttgacggca cgaacgtaga tccgtgtggg ctcgagcaaa atgtcggcga ccgtccgacc
     1861 aagttccttg acgaccgtct cgggcagtag tccgccgcgc tcgaaaacga tcttgcgcac
     1921 caagctgtag ccgttggagt gcagacccga gctcgccagg ccgatcaccc tgtcccctgc
     1981 tcggatgtcg cggccgtcca agatgtgctt gcgctcgacg accccgacgc aaaacccggc
     2041 caggtcgtaa tccccctcct tatagatatc aggaaggatg gccgtttcac cgcctaagag
     2101 tgcgcactcg gcctcgatgc agccgtcgct gatccctttg acgatctggg tggttaattc
     2161 cgggtcgtcg cggttcatgg cgacataatc aagaaacaag agaggctccg cgccggcgca
     2221 cagacaatcg ttgaccgaca tggcgaccag gtcgatgccc acagttgcat ggcggccggt
     2281 ggcgagggcg accttgagct tgcttcccac cccatcggtc gacgcgacca aaaccgggtc
     2341 gcgataggtc cgcgacaaga ggccgatctg gtcgtcgagg cgaaaaagtc ctgcaaagcc
     2401 gtccttccat tcgatcacgc ggggagaaaa ggtccggcgc atgagcggag gaagccggga
     2461 catcgtctcc tcatacacct tgaggtcgac gccggtcgag cgataatcga ggggttcgtt
     2521 catcggtgga cgaagcgcat cgagaaagcc gtgagcatac gggcgttgtg atcgggcaca
     2581 gcgtaccagc gttggatcat cgcagagccg accggatccg agcaatccca aggcagtact
     2641 tcaaagccag gtaagtattt caggcgatgg ggttatgacg gtttcgcggc cggggcggac
     2701 ccggttgtac cgcggaaaag cactcgatgt ggggggacta ttgcccgaag ttcttgatcg
     2761 gcaagccgga cagggctatc tttatgatga tgccaggccc gctgccaggg tcggcgtctc
     2821 gccagcctca aggatcctgc cgacacttgc ctttggttcc ccgctacaat caaaaatgga
     2881 gattcgcaac atgattcgga aaaggctcgc gttcctcttg ggcgcggggt ttctcgtcgt
     2941 gctgggcatt gccgccagtt ccagcctcag ccaagctcaa gacaaggaag agtcaccgct
     3001 cgagcacatc atggaaaagg tcaacaagca caacacaacg atcaccaagg gtacgcggaa
     3061 ccaggtgaat ttcgccaaat cgcaaaagga cgtcgagaaa agcgccaaag agctggtaaa
     3121 gctcgccaaa tccgccaagc cgatcaagga tgccatcaag aaggccaagg agctgccgga
     3181 agcccagaag aaatgggacc tgtacatcga cgatttaatc atgacctcgg aaaagctcgg
     3241 cgaggtgagc gcgaagccga gtgcgacggt tgcggacgcc aagagtgcct tcaactccgt
     3301 caagaaggcc tgcgctgatt gccacaagga tttccgcgtc gatgaaccaa gcaactgatc
     3361 ggacttctgg tctctggggg gtgcggtcca aacgcccccc gaggcgccct ttttgagggt
     3421 attgggtcgc ggcgttcgga ggccctctgc tgggtgaaat gacctgtctc gatctgacct
     3481 gctcgagcat cgcggaaaat ctggcgctcg atgaggcact tttgatcgag gccgatgccg
     3541 gccgaggaga tcggatcctg cggttctggg agccggctga ctttgccgtc gtgctggggg
     3601 catcgcgccg gatcgacgtg gacgtgcggt tgggcgcatg tcgggccga
//
LOCUS       DATAQL010001444         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3357208, whole genome shotgun sequence.
ACCESSION   DATAQL010001444 DATAQL010000000
VERSION     DATAQL010001444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3357208"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VHS97_24965"
     CDS             <1..753
                     /locus_tag="VHS97_24965"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX3451528.1"
                     /translation="VPVLVFPATRHSGVRLRMLSPEGVPLQRRFFCPRDGKEVRADEI
                     VRGYELDDSSYIVVSDRELEAIEPDKSREIDLREFVDLAEIAPAFLERGYYLTPLKEA
                     TKAYRLLADVMEKTRRAGIATFVMRDREYLVAIFAENGILCAETLRFHDEVKSPEDIG
                     LPKAVTVPRQRVSAFERAIDALSKETLAREELVDQDTRRLRAVIDKKRRAGKDLIRTD
                     HEAVDVNSEEDGDIDLLATIRQSLRHTGKGYR"
     gene            750..>3370
                     /gene="ligD"
                     /locus_tag="VHS97_24970"
     CDS             750..>3370
                     /gene="ligD"
                     /locus_tag="VHS97_24970"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX3451529.1"
                     /translation="MSLEQYRRKRHFGKTSEPEGGPLVKGGGQYLIQKHDASRLHYDF
                     RLELDGVLKSWAVPKGPSLDPAKKSLAVHVEDHPLEYGSFEGVIPQGEYGGGTVMLWD
                     RGTWEPEEDAAESYRRGKLVFRLNGERLKGRWALIRMGGKAGQDGKNWLLKKIDDEEA
                     RSGKDDDILSRMTTSVATSRTMEQIANNMKPVKKSTSKSRRPKKSAASERPDRKVRKP
                     SERNLLDPSKIAEARRTAMPAKIAPELPTLVDKIPQGTGWLHELKLDGYRMIGFVRDH
                     RASLFTRRGHDWTDRFPTIAAALRELEVKSAIFDGEIVMLKPDGTTDFQALQNMMRLG
                     NDAPIVYYVFDLLYYNGHDLRQVPLIERKKLLSQLIALGKHARSIRYSDHITGEGPQV
                     FSSACHKGLEGIVAKRADSPYVERRTADWVKVKCLKRQEFVIGGWTDPGGGRESLGAL
                     LVGYYRPPSDFVYCGRVGTGFTEQSLRELHKLLKPLEQRTSPFSNPPTGVAARGVIHW
                     VKPKVVAEVVFAEWTDDGLLRQASFKGIREDKVPTEITREVPADERVLEKHPSINDKQ
                     SEGQTMAPASTATSKGKANAATIIAGVRLTHPDRLVFPDEKITKRDLAVYYTDVAPYM
                     LPHIEGRPLSVVRCPDGSKKACFYQKHLAETMPEWVRGIPIKEKAGNATYIAVDGVAG
                     LISLVQMGVLEIHPWASREDRIDRPDRLILDIDPAVDRTWNDVVESARHVRERFNDLG
                     LVSFVRTTGGKGLHVVVPLARRSSWEELKDFAKSFADALVREKPKHYIAQSSKAKRAG
                     KLFLDYLRNEKGATAIASYSTRARPGATVATPLSWDELSASLRPDQFNVRTVPERLRT
                     MKQDPWTGFFDVRQAIT"
BASE COUNT          769 a          950 c         1014 g          637 t
ORIGIN      
        1 gtgcccgttc ttgtctttcc cgccacccgc cattcgggcg tccggctgcg aatgctcagc
       61 cccgagggcg tgcctctgca acgccgcttt ttttgcccac gggacggaaa agaggttcgt
      121 gccgatgaaa tcgtgcgcgg ctatgagctc gacgacagtt cgtatattgt cgtcagcgac
      181 cgggagctcg aggctatcga gccggacaaa tcccgcgaaa tcgatctgcg ggaatttgtg
      241 gacctggccg aaatcgctcc tgcgtttctc gaacggggat attatctgac tcctctcaag
      301 gaggctacca aagcgtatcg cctgctggcg gacgtcatgg aaaaaactcg gcgcgccggc
      361 atcgcgactt ttgtcatgcg tgaccgcgag tatctcgtgg cgatctttgc cgaaaacgga
      421 atcctctgcg cggagacgct ccggtttcat gacgaagtca agagtcctga ggacatcggt
      481 ttacccaagg ccgtgactgt tcctcgtcag cgggtctcgg cattcgagcg tgccatcgac
      541 gccctttcca aagagacgtt ggcccgcgaa gagcttgtcg accaagacac gcgccggctc
      601 agggcggtca tcgacaagaa gagacgcgca ggtaaggatt tgatccgcac agatcatgag
      661 gccgtggatg tcaattccga agaagatggc gacatcgatc tgctcgcgac aatccggcag
      721 agcctgcgac acacgggcaa ggggtataga tgagtttaga acaataccgc cgcaagaggc
      781 attttgggaa aaccagcgag ccagaaggtg gccctctggt caaggggggc ggccagtacc
      841 tgattcagaa gcacgacgcc agtcgcctgc actatgactt tcggctggag ctggatgggg
      901 tattgaaaag ctgggcggtc cctaaagggc ccagccttga ccccgccaag aaatcgttgg
      961 ccgtccatgt cgaagatcat ccacttgagt acggttcgtt cgagggggtg atcccgcagg
     1021 gcgagtatgg cggcgggacg gtgatgctct gggatcgtgg aacctgggag ccggaagagg
     1081 atgcggccga gagttaccgg cgcggcaagc tggtgtttcg gctcaatggt gagaggctca
     1141 aaggccgctg ggccttgatc agaatggggg gcaaggcggg gcaggacggt aaaaactggc
     1201 tgctcaaaaa gattgatgac gaagaggctc gatccggtaa agacgacgac atcctgtccc
     1261 ggatgacgac gagcgtcgcg acaagccgga ctatggagca gatcgccaac aacatgaagc
     1321 cggtaaagaa gtcgacctcc aagtcgcgac gaccgaaaaa gtccgccgcc tcggagcggc
     1381 ccgaccgcaa ggtgcgcaaa ccgagcgagc gcaatctcct cgacccttct aaaatcgcgg
     1441 aagcgagacg aacggcaatg ccggccaaga ttgcgcccga gctacctaca ttggtggaca
     1501 agatccccca gggaacgggg tggctgcacg agctgaagct cgacggctac cgaatgatcg
     1561 gctttgtgcg cgaccacagg gcgtcgctct ttacgcgccg agggcacgac tggacggatc
     1621 gattcccaac aattgccgcc gctctccgcg agctggaagt caaaagcgcc atctttgacg
     1681 gcgagatcgt gatgctcaag cccgacggaa caacggactt tcaagcgttg caaaacatga
     1741 tgaggctcgg gaatgacgcg ccgatcgttt actacgtatt tgacctgctt tactacaacg
     1801 ggcatgacct gcggcaggtt cccctgattg aacgaaagaa actattgtcg cagttgatcg
     1861 ctctaggcaa gcacgcacga tcgattcgtt acagcgacca cattaccgga gaagggcccc
     1921 aggtcttctc gagtgcctgc cacaagggct tagaagggat tgtcgccaag cgcgccgaca
     1981 gcccttacgt cgagcgccgg accgccgact gggtgaaagt caagtgtctc aaacgtcagg
     2041 aatttgtgat cggagggtgg accgacccag gcggtgggcg cgagtctctg ggggccttgc
     2101 tggtcggata ctatcgccct ccatccgact tcgtctattg cgggcgggtt ggaaccggct
     2161 ttacagagca gtcgctccgc gagttgcaca aactgctcaa gccgctggag caaaggacat
     2221 cgccgttttc caatccaccg accggagtcg cggcccgcgg cgtcattcac tgggtcaaac
     2281 cgaaggtggt cgccgaagtg gtattcgcgg aatggacaga cgatggtttg ttgcgtcagg
     2341 cttcgtttaa gggtatccga gaagacaaag tcccgaccga gatcacgcgt gaggttccgg
     2401 ccgacgaacg agtgctggaa aaacatcctt cgattaatga taaacaatcc gagggccaaa
     2461 cgatggcacc tgccagcact gcgacatcga aaggaaaggc gaatgccgct acgattatcg
     2521 cgggcgtgcg gctaactcat cccgatcgtc ttgtatttcc cgacgagaag attaccaaac
     2581 gcgatctggc ggtttactac acagacgtcg cgccctacat gttacctcac atcgagggcc
     2641 gaccgctttc cgtagtgcgc tgtcccgacg gatcaaagaa agcgtgcttc tatcaaaaac
     2701 acctggccga aacgatgccg gaatgggtac gcggaattcc catcaaggag aaagccggta
     2761 atgccaccta cattgccgtc gacggcgtcg cgggattgat ttccttggtg caaatgggcg
     2821 tcctggaaat tcacccctgg gcaagccgtg aggaccgcat cgaccggccg gaccggctta
     2881 tactcgacat tgatcccgcg gtggatcgca cctggaacga tgtggtggag tcagctcgcc
     2941 acgttcgcga gcgttttaac gacctggggc tcgtcagctt tgtgcggact acaggtggga
     3001 aaggcttaca tgtggttgta ccactcgccc ggcgctcatc gtgggaagaa ctcaaagact
     3061 tcgccaaatc attcgccgat gcgctcgttc gcgagaagcc caaacactac atcgcccagt
     3121 caagcaaggc aaagcgggcc ggcaaactct tcctcgatta cctcagaaat gaaaaaggtg
     3181 caaccgcgat cgcgtcttac tccacgcggg cgcgacccgg ggcgaccgtc gccactcctc
     3241 tttcatggga tgagttatcc gcttcgttgc ggcccgatca attcaacgta cggaccgtgc
     3301 ccgagcgact ccggacgatg aagcaagatc cctggacggg ctttttcgat gtccgccaag
     3361 cgatcacaag
//
LOCUS       DATAQL010001445         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_454535, whole genome shotgun sequence.
ACCESSION   DATAQL010001445 DATAQL010000000
VERSION     DATAQL010001445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454535"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..403)
                     /locus_tag="VHS97_24975"
     CDS             complement(113..403)
                     /locus_tag="VHS97_24975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX3451530.1"
                     /translation="MICVAVTYVIKPGHEDEAITLFAKMTEHTRAEPGCRMYLAHRSR
                     ADARRFFLYEQYDDEAALDAHRSAAYFEQYVKGGLFLILESRSPELYVPLTD"
     gene            649..1923
                     /locus_tag="VHS97_24980"
     CDS             649..1923
                     /locus_tag="VHS97_24980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX3451531.1"
                     /translation="MTASTDLASGLSVAPTDLIRAGFPALERVYQGHPVAYFDGPGGT
                     QVPQAVVEAMADYLIHHNANTHWAFPTSAETDVALAHARESLADFLNASPAEITFGLN
                     MTTLTFHLARALGRQLGAGDAIIVTELDHHANIDPWRALERERGITILKVRMVPETGE
                     LDWTDLDRCLTDHRVKLLAIGAASNALGTINDVARAIAMAREADALSFVDAVHYAPHQ
                     LVDVRDWGCDFLACSAYKFHGPHVGVLYGKRDRLAALDLPKLEPAPETVPERLETGTQ
                     NHEGIVGAAAAVNYFASLASEATAAESSSRRVRLQIVFDAFHERGSALLKQLWEGLVG
                     IKGVKLFGPPPSAPRTPTLAFTVAGRQAAEVSQLLANRGIFASHGNFYALTVVERLGL
                     ADDGLVRLGCACYTTDEEVARVIEAVGAIARA"
     gene            complement(1958..2992)
                     /locus_tag="VHS97_24985"
     CDS             complement(1958..2992)
                     /locus_tag="VHS97_24985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451532.1"
                     /translation="MSRWMRRILAGVLAVAALLADQPGQAAAEIKLGKDFLCGIVEKL
                     PPTTFDKTDKYRGTVHSYRLVAIDPQTRRFLIACQIEGEFRPPVTGPISERVGRSPQT
                     PPGWRKFRFDVKARVNIEPGADGAPRFRIEIDEVKKRDLDGFSGVLAKILGQYFDELV
                     TQIASGRASRLNKRLNDEILKRVSVFREYGVFQRIDYSPSEVVLHFDLTRFRSEGIVG
                     HVFALQRPGTVPLYRWLHPQDGSHYYTTFPNAPDRPNSVSEGISCHIYDHPVTATAAL
                     YCWRNRRDDLFTMSDTGENAARNGYRPCGIAGYIHRDPLPGTAPLYRFYDPVRHQHFY
                     TLHPNAEFAK"
     gene            3244..3639
                     /locus_tag="VHS97_24990"
     CDS             3244..3639
                     /locus_tag="VHS97_24990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451533.1"
                     /translation="MRLFRVRFGVRRLMVLVAVIAAFLWAGVEGVRLWKRVRYYDSLV
                     ATYAAMQRWAQEEAARKEARIASWADRPEDPRQERWAKELSRSRAQAAYWSALSNKYR
                     RLSSHPWEPNTPDPPPTAGFPTQPPPGTE"
BASE COUNT          726 a         1140 c         1199 g          771 t
ORIGIN      
        1 ggcagcttca atactttgtg gctgtcggtt ggcatcggca accggtcctt cctctcgggg
       61 atcatgttcg cgatcggcgc ggctgccgat cgactcggcg ccgtctgaat cgctaatccg
      121 tcaggggaac atagagttcc ggggagcggc tttcgagtat caagaacaat ccacccttga
      181 cgtactgctc gaaataggcc gccgaccgat gggcatcgag cgccgcttcg tcgtcgtact
      241 gctcgtagag aaaaaaccgt cgcgcatcgg ccctcgagcg gtgtgccagg tacatccggc
      301 agccgggttc agcgcgggtg tgctcggtca tcttggcgaa aagcgtgatc gcttcgtctt
      361 cgtgacccgg tttgatgacg taggtaacgg ccacgcagat catcggaacc tccagcctcg
      421 attctcaggt tgatcgcggt tacgattcta aatgcccttg cacgatcctg ggagggcgag
      481 cctctcggcg agcctcgtcg caacccggct cggacggagc ctcgccctcc cggaatcacg
      541 caaggccgtt tagggaatca tcctcgctga gacgacacga ggatgccatc cctcgccgct
      601 gaaactggcg gaatgaatcc gaacgacggc cgaatcgaag gtgtacccat gaccgcttcc
      661 accgatctag catcggggct ttccgtcgcc ccaaccgacc ttatccgcgc gggcttccca
      721 gcgctcgagc gcgtttatca aggtcatccc gtggcatact tcgatgggcc aggcggaacg
      781 caggtccccc aggcggttgt cgaggcgatg gctgattatc ttattcatca taatgcgaac
      841 acgcactggg cattcccgac cagcgccgaa accgacgtcg ccctggcgca cgcgcgcgaa
      901 tccctggcgg actttcttaa tgcgtctccg gcagagatca cgttcggtct caacatgacc
      961 acgttaacgt tccacctggc gcgcgccttg gggcgccagc ttggagcagg cgatgcgatc
     1021 attgtgaccg agctcgacca tcacgccaac atcgacccct ggcgcgcgct cgagcgtgaa
     1081 cggggcatca ccatcctcaa ggtgcgcatg gtcccggaga ccggcgagct tgattggacc
     1141 gacctcgacc gctgcctgac cgaccatcgc gtgaagctat tggcgatcgg ggcggcgtca
     1201 aacgcgctgg gaacgatcaa cgatgtggcc cgcgccattg cgatggcgcg cgaggccgat
     1261 gccctttcgt tcgtcgatgc cgtgcactat gcgccgcacc agctcgtcga cgtacgggat
     1321 tggggctgtg attttctggc gtgctcggcc tacaagtttc atgggccgca cgtcggggtt
     1381 ctctacggca aacgagatcg gctggcggcg ctcgatttac ccaagctcga gccggcgccc
     1441 gagaccgtgc ccgagcggct ggagacaggg acgcaaaacc acgaggggat tgtcggcgcg
     1501 gcggccgcgg tgaattattt cgcctcgctt gccagcgagg caacggctgc tgaatccagt
     1561 tcacggcgcg tccggctgca aatcgtgttc gacgcctttc acgaacgtgg ctcggcgctc
     1621 ttgaaacagc tctgggaggg tctcgtcggc atcaagggcg tcaagctgtt tggcccccct
     1681 ccctcggcgc cgcgcacgcc gacacttgcc ttcaccgtcg cggggcggca ggccgcggaa
     1741 gtcagtcagc tgctcgcgaa ccgagggatc tttgcttctc acggtaactt ttacgcgctg
     1801 accgtcgtcg agcgcctggg cttggccgac gacggtctcg tcaggctcgg ctgtgcctgc
     1861 tatacgaccg acgaagaagt cgctcgggtt atcgaagctg tgggcgcgat tgcgcgggcg
     1921 taatagtaaa atacatatat attaaactct atgcgctcta tttcgcaaat tcggcattgg
     1981 gatggagtgt gtaaaagtgt tgatggcgaa ccgggtcgta gaagcgatag agaggtgcgg
     2041 tcccaggtag tggatcgcgg tgaatatagc cggcgatgcc gcacggccga taaccgttac
     2101 gtgccgcgtt ttcgccggtg tcggacatcg tgaaaaggtc atcgcgccgg ttacgccagc
     2161 aataaagcgc ggctgttgcg gtcacgggat ggtcgtagat gtggcaggag atcccttcgc
     2221 tgaccgagtt gggtcgatcg ggggcgttag gaaaggtggt gtagtaatgc gagccatcct
     2281 ggggatggag ccagcgatag agggggactg ttcccggccg ctgaagggcg aaaacgtggc
     2341 cgacgatccc ctcggatcgg aaccgcgtca ggtcaaagtg gaggaccact tccgacggtg
     2401 agtaatcgat cctttggaac accccatatt ccctgaacac gctgacgcgt ttgagaatct
     2461 cgtcatttag ccgtttgttc aaccgcgatg cgcgcccgct ggcaatctgg gttaccagct
     2521 catcgaagta ttggcccaga atcttggcca gaaccccgga aaagccgtcc aggtctcgct
     2581 ttttgacctc atcgatctcg atccgaaatc ggggtgcgcc gtccgcgccg ggctcgatgt
     2641 tgacccgcgc cttcacgtcg aagcgaaact tccgccatcc tggcggtgtt tgcggactac
     2701 ggcccacgcg ctccgagatc ggaccggtaa ccggtgggcg aaactccccc tcgatctggc
     2761 aggcgatcaa gaagcggcgg gtttgcggat cgattgcgac gagtcgatac gagtggactg
     2821 tgcctcgata tttgtccgtc ttgtcgaagg tagtcggtgg gagtttttcg actatgccgc
     2881 agaggaaatc cttgccgagc ttgatttcgg ccgcagcctg tcccggctgg tcggctagta
     2941 gcgccgctac ggccaacaca ccggcgagga ttcgtcgcat ccaccgtgac attggtacca
     3001 cttcgctcca gccgtctgct gacggaccat cgacgggatg ggaaataccg ggtaagttgc
     3061 ggcgatggct ggagtgtccc tattcggccg gtcgcggccg tggttgtacc caagtttgac
     3121 aattatgact agatgaaacg tcctttccgg atcgccgatc cttcttgcta cactcggacc
     3181 gcagttaggt cgtcgtgcgg gcgaattggc ccatgtcccg atcagggctt gcaggacttg
     3241 cccatgagac tatttcgcgt gcggttcggc gtgcgtcggc tgatggttct agtggcagtg
     3301 atagctgcct tcttgtgggc cggcgtggaa ggcgtccgct tatggaagag agtgaggtac
     3361 tacgattcgc ttgtcgcgac ttacgcggcg atgcagcgct gggcacaaga agaggccgcc
     3421 aggaaagagg cgcggattgc cagttgggct gatcgtcccg aagacccacg gcaggaacga
     3481 tgggccaagg agttgtcgcg atcgcgcgct caggcggcgt actggtcggc cctaagtaac
     3541 aagtatcgtc gtttgtcctc gcacccctgg gagcccaata caccggatcc accgccgacg
     3601 gccggttttc cgactcaacc gcctccagga acggaataat tccaaaactc gagaacggaa
     3661 gcgcgtccac tcatcagtgg aaagaccccg gaactccagc gcgcaagcct gggcagcacc
     3721 gtccttatca gggagctacg agagcaggtt tcggtcgttc agcggaccat aacgcccgct
     3781 cgtcagaccg cgcgctcaac agccgcaagc atcgccgcca ggggctcggg gtcagc
//
LOCUS       DATAQL010001446        11700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2746867, whole genome shotgun sequence.
ACCESSION   DATAQL010001446 DATAQL010000000
VERSION     DATAQL010001446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746867"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VHS97_24995"
     CDS             complement(<1..243)
                     /locus_tag="VHS97_24995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3451534.1"
                     /translation="MKFSSPRYGGASAMKVWLTFDDGPHPEHTDRVLDVLQSFGISAT
                     FFVLGASVDRWGTRILERMGTEGHSIGNHAYSHTDLT"
     gene            complement(289..5097)
                     /locus_tag="VHS97_25000"
     CDS             complement(289..5097)
                     /locus_tag="VHS97_25000"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3451535.1"
                     /translation="MPQHEPDPKPDTKPESGAQARRGTEPRSADETEADSRLAVDRGA
                     GSQTGDPGLPLTEIEPPPASGSATEPDAGRTGAAGESPSPLNSGNSDLLAAAMRRWEE
                     TQRHNPDPSPKSFGIDKVPFLDEFHRGIRKGKPEPPPPGAMIPSAAATRDNQARPADM
                     DITRAQDALDVAPARTVRRADPVLIGRYRLIRIIGEGGYGRVFLAHDPDLDRNVAIKV
                     PIHVDSSKLLDVERYLREARVLARLSHPNIVPVYDVGRTQDENCFVVSKYMDGGDLAA
                     RISHCRLGFADSAGLVSLLCDALHYTHTQDMFHRDIKPANILLDSAGVPYLADFGLVL
                     RDENLGRGEEIVGTAAYMSPEQARGEGHRVDGRSDIFSMGIVLFELLTGRRPFRGGNH
                     LEVMKQIINTEPRPPRQIDDTIPWDLERICMKALSKRAAERYSTARDLAEELRHYLKS
                     ATWAVANPDASSKSGFPTSASLSGQWDTTTRPIRIVPKGLAAFDENDAEFFLGLLPGP
                     RGRDGLPDGLRLWRTRIESTDTDKTFRVGLIYGPSGCGKSSLVKAGLIPLLGRHVASI
                     YVESSTLETESRLLRGIRKRFPSLPVDTGLVGSLTLLRQGHGLENGYKLLLVLDQFEQ
                     WLCAQKGDEGTELIAALRQCDGEHVQALCLVRDDFWMAANRFMTHLEIDLVSDRNVAA
                     IDLFDPKHARKVLEACGRAYEVLPPTGDLTKEQNTFLDQAVTGLAQDGRVVPVRLALF
                     AEMVKGKPWSPATLRDLGGMDGVGVKFLEDTFSSSRSNPKHHYHQKATQAILKSLLPD
                     TNTDIKGRMRSIDDLRRVSGYAERPVDFVELIKILDNDLRLITPVDPEGSIHDEPRSL
                     PSGEGSYQLTHDYLVHSMRDWLTCKQKETRRGRAEIRLGTITSYWRDRPGTRRLPALL
                     EWLDIVCYTQSRLWSESERRMMRAATRHYAIRAVAAAAVVVVLSVAIKEYRGRDRAQA
                     VISKLLVADTGQLSEVIKEIDDDLGRTRLTLDRIAGDPNRSAKDRLHAGLALLPFEKA
                     HDDYLFERLLGAEPDELRVFGERLRSRRSDFLYRLWATASDATTDRKHKLRAACALAM
                     LDSGDTRWKVLAGDIAGSLVLDENAFRLERWLDALRPVRKSLLEPVAAIFRDRARPGD
                     ERFKATVILEQFAADDPEFLVTLIRDADLRQYKILMPVLRPHRDSVLESLIAELDRAP
                     ARGASDQAKNELARQQANCAVALLLLGRPHAVWSLLRESPEPRVRGFLIDRIERLGVD
                     PSMIFDRLRDENDLSIRRALLLVLADYRGKGLPTADGESLETSLLEMFRTDPDSGIHS
                     AAELVLRRCGHGNKVDALAKSLKSVKLNSGARWYVNSEGYTMVVIDPRGKDPALSCGR
                     PVDRVFEMAAKEVSVEQYLRFRNKIEHNAEHSPTTSCPINCVSWYDASAYCRWLSEQE
                     KVPPNQMCYPPTSDIKEGMRLPPDYLQRTGYRLPTEAEAEYACRAGTVTSRFFGSADS
                     LLPAYAFFRDTSGNHSWAVGSLRPNDLGLFDILGNILEWCQESRSPVLKLMDVEDTEP
                     VSNTIDRVLHGGSYEKVIELLQSDRSEHALPPVKFNSIGFRVARTHPSGR"
     gene            complement(5510..8401)
                     /locus_tag="VHS97_25005"
     CDS             complement(5510..8401)
                     /locus_tag="VHS97_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX3451536.1"
                     /translation="MSRSRYLVGIDLGTTNCAVAYVDTHGRERPSADIRSFEVPQLVA
                     AGETAPRAMLPSFLYLPGEHELPAGATRLPWNEGADRLIGEFARLQGAKVPSRLVSSA
                     KSWLCHAGIDREADILPWGSPPEVKKISPVEASAAYLRHIRDAWNDTFAREDPSNRLE
                     RQEVVLTVPASFDEAARELTIEAAKRAELATITLLEEPQAAFYCWIVSHQDRWQREVR
                     AGELILVCDIGGGTTDFSLITVVETPTGPGFRRVAVGDHLMLGGDNIDLALAHHVEKK
                     LGGARLDSEQWSALRFACRTAKEKLLGDQQSGIERWPVTIAGRGSKLIGGSIQSELTR
                     AEVETIALDGFFARVTRAEDPQRTAKIGLQEFGLPFVADPAVSKHLSFFLRRHRAEAI
                     APEGHQPADRPARPDAILFNGGALTPESVRNRIVQVIGSWFADDPGPPFTPRVLTNIS
                     LDLAVAQGAAYYGVVRRGGGIRIGGGTARSFYVGLETTAASKPWLCVVPRDAQEGEEI
                     TIAGHDFDLLMGQPVVFPLASSSVRPDDKPGALVKADPDSIRELPPLQSLMRVSRKAK
                     AERVPVSLAARVTEVGTIELWCQSRTDNRRWRLQIQLRGPAGQLAAEGPVTGNESDRV
                     VIEQSEVDAAVAAIRLAFESPTASSPAGSSEEGGPSRLVKRLEEVLDVPRDQWPPSAL
                     RACWEPLLELVEKRQRSPRHESRWFNLAGFFLRPGRGFPLDELRLKALWPLFHQGVKH
                     TRDVQCWAEWWILWRRVAAGLSRPHHEEIFRRLTPFLLPAKGTSPAKKAGRPKPEPHE
                     LAEMWRCAASLERLTPEIKESLGNALLKDAAHETLGAHVLWCVGRLGARVPLYGPANT
                     VVRKETAERWASALLDRSFSLGREAADAIFALAQVARVASDRARDLDNGLRAEVIARL
                     HELGADETVLRPVREFHELESAQQGQALGDSLPIGLRLLSDAGAPADSG"
     gene            complement(8439..11180)
                     /gene="leuS"
                     /locus_tag="VHS97_25010"
     CDS             complement(8439..11180)
                     /gene="leuS"
                     /locus_tag="VHS97_25010"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX3451537.1"
                     /translation="MPAYHPQRIEPKWQAEWEKNKTFRAIDLDPSRPKLYVLDMFPYP
                     SGAGLHVGHPEGYTATDIVCRYKRMRGFNVLHPMGWDAFGLPAEQYAVQTNTHPRITT
                     QANIDNFRRQIKSLGFSYDWDREIDTTDPDYYKWTQWIFLKIHDTWYDPDFEWTDRHG
                     RARKGKGRPIAELPIPAGTEYPEAYRDSRRLAYRALVAVNWCPGLGTVLANEEVIDGK
                     SERGGFPVVRMPLTQWMLRITAYAERLIEDLEDVDWPKPIKDMQRNWVGRSEGAEVDF
                     PVRVGAGQETELIRVYTTRPDTLFGATYMVLAPEHPLVELISTDAQRPDVERYRHAAA
                     TKSDLDRTDLAKTKTGVFTGAHATNPVNGQLIPVWIADYVLMGYGTGAIMAVPGHDQR
                     DFEFAQAFDLPIVRVVASSAAEADAPLARAEAEPGVAVHSHNDDITLDGLATDLAKSA
                     ITDWLEKRGLGKKTTNYKLRDWLFSRQRYWGEPFPVVLDANDRTLPVAESELPVRLPE
                     LDDFKPTGKPDPPLSKATEWVRYSEKYRRETNTMPQWAGSCWYYLRYIDPHNDKLPWD
                     PEKERYWLPVDLYVGGAEHAVLHLLYSRFWHKVLFDRGLVSTPEPFQKLVNQGMILGE
                     TEYTAYRDPEGRWIRPEESGRGDVIAVKLEEDLVVKKAEGFVLAADTSVRVDARAHKM
                     SKARGNVINPDAIVAEYGADSLRLYEMFMGPLEAVKPWSMKGVEGVYRFLGRAWRMIV
                     DAEADLVRLDPRVQPAQLSVEQAKVVARTIAAVTDDFEALRFNTAISRLMEFVNFFTG
                     EETRPKAAMENFALMLAPMAPHMAEELWSLLGHDKSLAHEPWPQFDPALLKDEEIEIP
                     VQLNGKLRGRIVVAAGADGAEIERAARADSKIAVLLGGKTVKKVVVVPGKLVNFVVG"
     gene            complement(11381..>11700)
                     /locus_tag="VHS97_25015"
     CDS             complement(11381..>11700)
                     /locus_tag="VHS97_25015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451538.1"
                     /translation="RFHSRPTSQIGSQSRWYCKRDAECTIDLSTMIERYSWLSTQVYT
                     PFGRSAEFARLFFGFAQVKSPCFRKVVCQKFQTSRNICTFVYNATHWYVQKIYPTGAI
                     GRV"
BASE COUNT         2189 a         3689 c         3525 g         2297 t
ORIGIN      
        1 ggtgaggtcc gtgtgcgagt atgcgtggtt acctatggag tggccctcgg ttcccatccg
       61 ttccaggatc ctcgttcccc accgatccac gctcgcgccc aaaacaaaga aggtggcgct
      121 gatcccgaac gactggagca catcgagaac cctgtcggtg tgctcgggat gtggcccatc
      181 gtcaaaagtc agccacacct tcatggcaga ggcgccgcca tagcgaggcg aggaaaactt
      241 cacggcgatc tccgtcttcc tcgaacgagc tggaaaatcg cgggaaagtc atcgccccga
      301 tgggtgagtc ctggcaacgc gaaaaccaat cgagttgaac ttcacaggcg gaagtgcgtg
      361 ttcggagcga tccgattgca agagttcgat gaccttttca tatgagccgc cgtgcagcac
      421 gcggtcgatc gtgttgctca ccggttcggt gtcttccaca tccatgagtt ttaggaccgg
      481 agaacggctc tcctggcacc actcgagaat gttgcccagg atgtcgaaca ggcccaggtc
      541 gttcggccga agggagccta ccgcccagga atggttcccg gaggtgtcgc ggaagaaggc
      601 gtaagcaggg agcagagaat cggctgagcc gaagaaacgg ctcgtcactg tgccggcgcg
      661 acaggcatat tcggcttcag cctcggtcgg gagacggtag ccggttcgct ggaggtagtc
      721 cggaggaagc cgcataccct ccttgatgtc gctggtcgga gggtaacaca tttggtttgg
      781 cggcaccttt tcctgctcgc tgagccaccg gcaataggca ctcgcgtcat accagctcac
      841 gcaattgatc gggcagctcg ttgtcggact gtgctcggcg ttgtgctcaa ttttgttgcg
      901 gaaacgaaga tactgctcga ccgaaacctc tttggcggcc atctcgaaga cgcgatcgac
      961 tggcctgccg cacgacaatg ccgggtcttt ccctctgggg tcgatcacca ccatcgtgta
     1021 cccttcgctg ttgacgtacc atctggcgcc cgagtttagt ttcacactct ttagactttt
     1081 ggccaacgca tcgaccttgt tgccgtgacc acacctgcgc aaaaccaact ccgccgcgga
     1141 gtggatgccg gagtccgggt cggtccgaaa catttccagg agactggtct cgagtgactc
     1201 tccgtctgcg gtgggcaaac ccttgccgcg gtagtcggcg agtacgagca aaagcgctct
     1261 gcgaatcgac aggtcattct cgtcccttag ccgatcaaag atcatgctgg gatcaacgcc
     1321 cagccgctcg attcgatcga tcagaaatcc ccggactcta ggttccggcg actctctgag
     1381 caaagaccag accgcgtggg gccgccccaa gagaagcagg gcgaccgcgc agtttgcctg
     1441 ttgccttgcg agttcgttct tcgcttggtc cgaagcacct cgagccggcg ctcgatccag
     1501 ctctgctata agcgactcga ggaccgaatc gcggtgcggc ctcaacacag gcataagaat
     1561 cttgtattgc cgcaggtcgg cgtccctgat caacgtgacc aggaattcag gatcgtcggc
     1621 ggcaaattgc tcgagaatga cagtcgcttt gaaccgctcg tccccgggtc gcgcgcggtc
     1681 gcgaaagatc gccgccacag gttctaagag agacttgcgg accggtcgca aggcgtcgag
     1741 ccaccgctcc agacggaagg cgttttcgtc gagcaccagc gagccggcaa tgtcacccgc
     1801 cagcaccttc cagcgagtat cgccagaatc gagcatggcc agcgcacagg cggcccgaag
     1861 tttatgcttg cgatccgttg tcgcgtcgct cgctgtggcc cagagccggt acaggaaatc
     1921 tgatcgccgg gaccgaagcc gctcgccgaa tactctcagt tcatcgggct ccgcaccaag
     1981 cagtcgctcg aagagataat cgtcgtgagc cttctcaaac gggagaagag caagaccagc
     2041 atggagtcgg tcttttgccg aacgattcgg gtcgccagcg atccggtcaa gcgtgagcct
     2101 ggttcgaccg agatcatcgt cgatctcctt gatcacctcg gacagttggc cggtgtcggc
     2161 gaccagtaac ttggagatga ccgcttgggc gcgatctctg ccgcggtact ctttgatcgc
     2221 gaccgataag acgacgacaa cggcagccgc ggctaccgcg cggatggcgt agtgccgtgt
     2281 cgctgcccgc atcatacggc gctcggactc ggaccataag cgactttgcg tatagcacac
     2341 aatgtcaagc cattccaaga gcgccgggag ccggcgcgtc cccggccgat cgcgccagta
     2401 cgatgtgatc gtgcccaggc ggatctccgc gcggccccga cgcgtctcct tttgcttgca
     2461 cgtcagccag tcccgcatgg aatggaccag gtaatcatgg gtcagttgat atgacccctc
     2521 gcccgaaggg agcgaccttg gctcgtcgtg gatcgagcct tcggggtcga ccggcgtgat
     2581 caagcggagg tcgttgtcga ggattttgat caattcgaca aagtcgaccg gccgctcggc
     2641 gtatcccgaa acgcgcctga gatcgtcgat cgaacgcatc cgacccttga tgtcggtgtt
     2701 agtgtcggga agcagagatt tcaagatcgc ttgcgttgct ttttgatgat aatgatgttt
     2761 agggttggac cggctcgagc tgaacgtgtc ttcgagaaac ttcacgccga ccccatccat
     2821 gccgccgaga tcgcgcaatg tcgccggcga ccagggcttg cccttgacca tttcggcgaa
     2881 cagcgcgagc cgcaccggca cgacgcggcc gtcttgcgcg aggccggtta ccgcctggtc
     2941 gaggaaggta ttctgttctt tggtcaggtc gccagttggc ggcaatacct cgtaagcgcg
     3001 cccgcaagcc tccagcacct tgcgcgcatg tttcgggtcg aataagtcaa tcgccgccac
     3061 gtttcgatca gagaccaggt cgatctcgag atgagtcatg aaccggttcg ccgccatcca
     3121 aaaatcatcc cgaaccaggc agagggcttg aacgtgttcg ccatcgcact gacgcaatgc
     3181 ggcaatcagc tcggtcccct cgtccccttt ttgcgcgcaa agccactgct cgaactgatc
     3241 gagaaccaag agcagcttgt agccgttttc gagaccatga ccttgccgca gcagcgtcag
     3301 cgacccgacc agcccggtgt ccaccggcag gctggggaac cgtttgcgga tgcccctcag
     3361 tagtcgcgat tccgtttcca gcgtgctcga ctcgacatag atcgaggcaa cgtgccgccc
     3421 caaaagcgga atcagcccgg ccttgacgag cgacgacttg ccgcacccag aaggcccgta
     3481 gataaggccg acccgaaatg ttttgtccgt gtcggtggac tcgattctcg ttctccacaa
     3541 ccggagcccg tccggcaatc catcgcgacc ccgcgggccg ggcaagagcc cgaggaagaa
     3601 ttccgcgtcg ttctcatcaa acgcggcaag tcccttgggg acgatccgga tggggcgggt
     3661 ggtcgtgtcc cactggcccg acagcgaggc gcttgtcggg aatccggact tggaggacgc
     3721 gtccggattc gcaaccgccc atgtggcgct cttgagataa tgccgcaact cctcggcgag
     3781 gtctcgcgcg gtcgaatacc gctcggcagc tcgcttcgaa agcgccttca tgcaaatccg
     3841 ttcgaggtcc caggggatcg tgtcgtcgat ctgccttggc ggccgcggct cggtgttgat
     3901 gatctgcttc atcacctcca ggtggttgcc accccgaaac gggcgccgcc cggtcaagag
     3961 ctcgaacaag acgatcccca tgctgaagat gtccgaacgg ccatcgaccc tgtgcccttc
     4021 gcctcgagct tgctccggac tcatgtaggc agccgttccc acgatctctt ctccgcggcc
     4081 caggttttcg tcccttaaaa ctagcccgaa gtcggcaaga taaggtacgc cggcggagtc
     4141 gagcaggatg tttgcgggct tgatgtcgcg gtgaaacatg tcttgagtgt gggtgtaatg
     4201 aagcgcatca cagagcaagg agacgaggcc ggcggaatcg gcgaaaccaa gccggcaatg
     4261 actgatcctg gcggccagat cgccgccgtc catatacttc gaaaccacga aacaattttc
     4321 gtcctgggtg cggcccacgt cgtacactgg cacgatgttc gggtgtgata accgggcaag
     4381 gacgcgcgcc tcgcgcaagt agcgctcgac gtcgagcaac ttcgaggagt cgacgtgtat
     4441 aggcaccttg atcgccacat tgcggtcgag gtcgggatca tgcgccagaa aaacccgccc
     4501 atatcccccc tcgccgatga tccgaatcaa ccggtagcga ccgattaaga ctgggtcggc
     4561 ccggcgaacg gtccgcgccg gcgcgacatc aagcgcgtct tgtgccctcg tgatgtccat
     4621 gtccgccggc cgcgcctggt tgtctcgggt cgccgcggcc gacggaatca ttgcccccgg
     4681 cggcggtggc tctggcttcc ccttcctgat cccacgatgg aattcgtcta agaatggtac
     4741 tttgtcgatc ccgaacgact tgggggatgg atcagggttg tggcgttgcg tttcctccca
     4801 acgacgcatc gcggcagcga ggaggtcact gttgccgctg ttgaggggcg acggtgattc
     4861 gccggcggcg cccgttcgcc cggcgtcggg ctcggtggcg ctgcccgagg ctggcggagg
     4921 ctcgatttcc gttaatggca gaccgggatc gccggtttgg gagccggcgc cgcgatcgac
     4981 cgcgagccga gaatcggctt cagtctcgtc ggcacttcgt ggttccgttc cgcgacgggc
     5041 ttgcgcgcct gactcgggtt tggtgtcagg ttttgggtcc ggctcatgtt gcggcatcag
     5101 gcgctccgct gggtcgtcgc gcttggtcag atcggcgcgg ggaaaccact gagccggcgc
     5161 gtcgcgcccg accaaagctt accggccgaa attcggcgca ttgccaatac tcgctctcaa
     5221 actatccggc cgaaatcatc agcttacgga cttcaaaccg ccgttgctct gtcatggcag
     5281 agtcaatcca tcacgtcagt cgttcgttcg agatcgtggc agtcacggca agcgcgccca
     5341 gagacggtgc gccgagccat tcagatgggt cgtaattatg ccgcctcgga caatcctcga
     5401 tgctgtgtat ggtaccgaac tgtctcgatt cactcaacga aatccgcttt tcttcccaac
     5461 tttcttgccg tggaccgacg gtgcgcacac caaggacccg aaatgtggtc tatccggaat
     5521 cggcgggagc gccggcatcg cttagcagcc gcaaaccgat cggcagtgaa tcgcccaagg
     5581 cctggccttg ctgggctgat tcgagctcgt gaaactcgcg gaccggacgc aatacggttt
     5641 cgtcagcccc cagttcgtgc aatcgtgcga tcacttccgc gcgtaagccg ttatcgaggt
     5701 cgcgcgcgcg atcgctggcg acccgcgcca cttgcgccag cgcaaagatc gcatcggccg
     5761 cctctcgacc caggctgaac gaacgatcca gtaacgcgct ggcccagcgc tcggctgttt
     5821 ccttgcgaac gaccgtgttg gccggcccat agagtggaac ccgagcaccc agccgcccca
     5881 cacaccataa gacatgtgcc cctagcgttt cgtgggccgc atcctttaag agagcattgc
     5941 ccaacgattc cttgatctcg ggcgtcagac gctcgagcga cgcggcacat cgccacatct
     6001 cggcaagctc gtgcggctca ggtttcgggc gtccggcctt cttggcgggg cttgtcccct
     6061 tggcggggag taaaaagggg gtgagccgcc gaaagatttc ctcgtggtgc ggacgcgata
     6121 gaccagccgc caccctacgc cagaggatcc accactcggc ccaacactgc acgtcccgcg
     6181 tgtgcttgac accctggtga aagagcggcc agagggcctt caatctcagc tcgtcgagcg
     6241 ggaatcctcg tcccggacgg aggaagaatc cggcgagatt gaaccatcgc gattcatgac
     6301 gagggcttct ctgacgcttt tcgaccagtt cgagcaatgg ctcccagcaa gcccgcagcg
     6361 ccgacggggg ccactgatcg cgcggcacgt ccagaacctc ttcgagccgc ttcaccagtc
     6421 gggatggacc cccttcttca ctggaacctg ccggtgatga tgcagtaggc gattcgaatg
     6481 cgagacgaat ggcggcgacg gcggcatcga cctcagattg ttcgatcaca acccgatctg
     6541 attcattccc tgtgacgggg ccctccgccg cgagctgacc agctgggccg cgaagctgga
     6601 tttgcagccg ccagcgcctg ttgtcggtcc gcgactgaca ccatagctcg atcgtcccca
     6661 cctcggtgac ccgcgccgcc aggctgaccg gcacccgctc agccttggct ttgcggctca
     6721 cgcgcatcag gctttggagc ggaggaagct cacggatcga gtcggggtcg gctttgacga
     6781 gtgccccggg cttatcgtcg ggccggaccg aactgctggc aagcgggaag acaaccggct
     6841 gccccatcag gaggtcgaaa tcgtggccgg cgatcgtgat ctcttccccc tcctgggcat
     6901 cgcggggcac cacgcaaagc caaggtttac tggctgcggt ggtttcgagc ccgacgtaaa
     6961 atgaccgggc cgtccctccg ccgatgcgga tgcctcctcc gcgccgcacg acgccgtagt
     7021 aggctgctcc ctgggccacg gccagatcga gagatatgtt ggtgagaacc cggggcgtga
     7081 aaggagggcc cggatcatcg gcgaaccacg agccgatcac ctggacgatg cgattgcgaa
     7141 cgctttcggg cgtcagcgcg ccgccgttga acaagatggc gtccggccgc gccggccgat
     7201 ccgccggctg gtgcccctcc ggcgcgatcg cctcggcccg gtgcctccgc aggaagaagc
     7261 tgaggtgttt ggaaactgcc ggatcggcga caaatggcag cccaaactct tgcaagccaa
     7321 tcttggctgt acgctgggga tcctcggccc gagtcacacg agcgaagaaa ccatcgagcg
     7381 cgatcgtctc gacctcggct cgcgtcagct cagactggat gctgccgcca atcagctttg
     7441 atccgcggcc ggcaatcgta accggccagc gctcgatgcc ggactgctgg tcccccagca
     7501 acttttcctt ggccgtccgg caggcaaacc gcagggcgct ccattgctcg gagtcgagcc
     7561 gcgcgccccc cagttttttc tcgacgtgat gcgccagtgc caggtcgatg ttgtcgccgc
     7621 ccaacatcag gtgatcgccg acggcgacgc ggcggaaccc cggcccggtt ggcgtctcga
     7681 cgaccgtgat caggctgaaa tcagtggtgc caccgccgat gtcgcagacc aggatcagct
     7741 cgccggcccg aacctcgcgc tgccagcgat cctggtgcga aacgatccag caataaaacg
     7801 ctgcctgcgg ttcctcgagc agggtgattg tggcgagctc cgcccgcttg gccgcctcga
     7861 tcgtcagctc gcgagccgcc tcgtcaaaag aggccggcac cgtcaacacg acctcctggc
     7921 gttccaagcg atttgaagga tcttccctgg caaacgtgtc gttccaggcg tcgcggatgt
     7981 gccgcagata cgcggctgac gcctcgaccg gcgagatttt cttgacctcc ggcgggctgc
     8041 cccacggcag tatgtccgcc tcgcggtcga tcccggcatg gcacaaccag ctcttggcgc
     8101 tactgacgag ccggctgggt accttggcac cctggagacg cgcgaattcg ccgatcaggc
     8161 ggtcggcacc ttcattccag gggagccggg tcgcgccggc gggaagctcg tgttcgcctg
     8221 gcaagtaaag aaacgaaggg agcatcgcgc ggggagcggt ctcccccgcc gcgaccaatt
     8281 gcggcacctc aaaactgcgg atgtcggccg acggccgctc tcggccgtgc gtgtccacgt
     8341 acgcgaccgc acaattggtc gtacccaggt cgatcccgac gagatagcgc gaccgactca
     8401 ttccaccgcc tcacgaatga ccgttcgacc gcaatcgact atcccaccac gaaattcacc
     8461 agcttgcccg gcaccacgac caccttcttg accgtcttgc ctccgagcaa gacagcaatc
     8521 ttggaatcgg cgcgcgcggc ccgctcgatc tccgcgccat cggcaccggc cgccacaacg
     8581 attcggcccc gcagctttcc gtttaactgg acgggaatct ctatttcctc gtcctttaac
     8641 agcgctgggt caaattgagg ccagggctca tgggccaggc tcttgtcgtg acccaagagc
     8701 gaccagagct cttccgccat gtgcggggcc atcggggcca gcatgagcgc aaagttttcc
     8761 atcgccgcct tgggccgcgt ctcctctccg gtgaagaaat tcacaaattc catcagccgg
     8821 ctgatcgccg tgttgaaccg cagggcctcg aaatcgtcgg tgaccgctgc gatcgtccgc
     8881 gcgacaactt tggcttgttc caccgacaat tgggcgggct gcaccctcgg atcgagccgt
     8941 actagatccg cttcggcatc tacaatcatt cgccatgccc tgcccagaaa gcggtagacg
     9001 ccctcaactc ccttcatact ccacggcttg accgcttcaa gcggtcccat gaacatctcg
     9061 taaagtctca ggctgtcggc gccgtattcc gccacgatcg cgtcgggatt aatcacgttg
     9121 ccgcgcgctt ttgacatctt gtgcgcgcgt gcgtctaccc gcacgctggt gtcggccgcc
     9181 agcacgaaac cctctgcctt cttcacgacc agatcttcct cgagtttgac cgcaatgacg
     9241 tcgccccgac cgctttcctc cggccggatc cagcgaccct cggggtcgcg ataggcggtg
     9301 tactcggtct cccccaggat catgccctgg ttgaccagtt tctgaaaggg ttcgggtgtg
     9361 ctgacgagtc cccggtcaaa cagtaccttg tgccaaaacc gactgtataa gaggtgcagc
     9421 acggcgtgct cggcgccgcc gacatacagg tccaccggca gccagtaccg ctctttttcc
     9481 ggatcccatg gcagcttgtc gttgtgcggg tcgatgtacc gcaggtaata ccagcacgaa
     9541 ccagcccatt ggggcatcgt attggtctcg cgcctgtatt tctcggaata gcggacccat
     9601 tcggtcgcct tggagagggg cgggtcgggc ttaccggtcg gcttgaaatc atcaagttcg
     9661 ggtagccgca cgggcagctc tgactcggcc accggcagcg tgcggtcgtt ggcatccagc
     9721 accacgggaa atggctcgcc ccagtaccgc tggcgcgaaa acagccagtc acgcagctta
     9781 taattcgtgg tcttcttgcc cagtccacgt ttctccagcc agtcagtgat cgctgatttg
     9841 gccaggtcgg ttgcgaggcc gtcgagcgtg atatcgtcgt tgtgcgagtg gaccgcgacg
     9901 ccgggctccg cttcggcacg tgcgagcgga gcgtctgctt ccgccgcgct cgacgccacg
     9961 acccggacga ttggcagatc gaaagcctgc gcaaactcga agtcgcgctg atcgtggccc
    10021 gggactgcca tgatcgcgcc ggtgccatat cccatcagga cgtagtcggc gatccacacg
    10081 gggatcaatt gaccgttaac cgggttggtg gcgtgcgcgc cggtaaagac gccggtcttg
    10141 gtcttggcca gatcggtgcg gtccaggtcg ctcttggtcg ccgcggcgtg gcggtacctt
    10201 tcgacgtccg ggcgctgggc atcggtgctg atcagctcga ccagcggatg ctcaggcgcg
    10261 agtaccatgt aggtcgcgcc aaacagcgta tcggggcgcg tcgtatagac gcggatcagt
    10321 tcggtttctt gcccggcgcc gacccgcacg ggaaaatcga cctcggctcc ttcgctgcgg
    10381 ccgacccagt tacgctgcat gtctttgatc ggcttgggcc agtcgacgtc ctccaagtct
    10441 tcaatcagac gctcggcgta ggccgtgatc cgcagcatcc attgcgtgag cggcatgcgc
    10501 acaaccggaa agcccccgcg ttcactcttg ccgtcgatca cttcctcatt ggccagcacc
    10561 gtacccagcc cgggacacca attgacggca acaagggccc gataggcgag tcgccgcgaa
    10621 tcgcgatagg cctcaggata ctccgttccc gccggaattg gcagctcggc aattggccgg
    10681 cccttcccct tgcgcgcgcg gccatgccga tcggtccatt caaagtcggg gtcgtaccaa
    10741 gtgtcgtgga tcttcagaaa gatccactgc gtccacttgt agtaatcagg atcggtcgta
    10801 tcgatttcgc gatcccagtc gtaggagaag ccgagcgact tgatctggcg gcgaaaatta
    10861 tcgatgttgg cctgggtggt gatcctggga tgagtatttg tctgaacggc gtattgttcg
    10921 gccggtagtc cgaaggcgtc ccagcccatc ggatgcaaga cgttaaaccc gcgcatcctc
    10981 ttgtatcggc aaacgatgtc agtggccgtg taaccctcgg gatggcccac gtgcagcccg
    11041 gctccgctgg gataggggaa catgtcgagc acatagagtt tcgggcggga tgggtcgagg
    11101 tcgatcgcgc gaaatgtctt attcttttcc cattcggctt gccacttggg ctcgatccgc
    11161 tgtgggtggt acgcaggcat tgctctggct ctctcccccg cgacctccga ggtagctcgc
    11221 gttcgctcat ggccgctggg tgcactgttg ataaaaaaag cccgccgcgg gttcgggggc
    11281 gataccgtat gatcggtcat cctagcatac cgattcggcc tgactcgtgc cactttccgg
    11341 ctgaggctcg cgtcgccacc acatcagccg attcgagtgg tcaaaccctg ccgattgcac
    11401 ccgtcgggta gattttctgg acataccaat gcgtggcgtt gtagacgaaa gtacagatat
    11461 tgcgcgaagt ttggaacttt tgacaaacga ccttgcggaa acacgggctt ttgacttgag
    11521 caaaaccgaa aaataatcgc gcgaattccg cgcttcggcc aaacggcgtg tagacttgag
    11581 tcgaaagcca ggaatatcgc tcgatcatcg tggacaggtc gatcgtgcat tcggcatccc
    11641 gcttgcagta ccagcgtgat tgagatccga tctgggatgt cggacgagag tggaatctgg
//
LOCUS       DATAQL010001447         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_150504, whole genome shotgun sequence.
ACCESSION   DATAQL010001447 DATAQL010000000
VERSION     DATAQL010001447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150504"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1658
                     /locus_tag="VHS97_25020"
     CDS             360..1658
                     /locus_tag="VHS97_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3451539.1"
                     /translation="MGQGKSATCLSVTRRDFLRVGGAGLGSIGLGLTDFDALAGIERQ
                     PDRSVILLLLVGGPSQLETWDPKPDAPAEVRGPFRSIATRCPGVRFCEHLPRMAARMD
                     RFAVVRSVHHDLAPIHETGYQLLQTGRLCRAGEQYPHIGSVIARLDGAKNGLPAAAIL
                     PRPIASTGVDIPQGQSAGWLGAAYDPFHLNADPAAPEFCAAAALDRACTAFDRTMNNS
                     GCAQVPVHNTLSLVRRHTRNPFDIAQEDDRVREEYGRTSFGQSCLLARRLVEAGVRFV
                     TVNMFDTVFNRAGWDCHGAAPFSTFGDYASFVLPTFDQTFSALIDDLDRRGRLESTLV
                     IAAGEFGRSPRINASGGRDHWPGVWSVVLAGGGVRGGQVLGASDAHATAPVDRPVTPE
                     DLAVTIYHSLGIGENASIIGPDGQSRPVLENGSLLRELFA"
     gene            complement(1712..3445)
                     /locus_tag="VHS97_25025"
     CDS             complement(1712..3445)
                     /locus_tag="VHS97_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX3451540.1"
                     /translation="MPSNYQTSTYTKHSIPGPLFTRVGKSFVLGQFAQKPDSDGALTI
                     LHGPPLHKGGKGKASATPPSLSRYLFFVVSAFLSTALSLCTVALGDDGQTKDKKDTPA
                     PQTNTTAGKEGGARAEPKNDGSREAKPEVKIEPKRADQPISFIRDVAPVLVENCIACH
                     NPRKSESKYVMTTFAQLAKGGQQGEGTTLEPGKPEESNLVDVLLPDAQPRMPFKQDPL
                     PPEKIALIKRWVAEGAKYDGTSPGEDWTIVLRKTQQVAIPAAYPVTVPITALEFSPDG
                     SAIAASGYHEITFWKTSDGTLDRRLTGLAERVYDIAYSPDGKWLATASGDPGVYGVAK
                     LWLAEPGGGGKPVRDLAETQDVVFAVAFSPDSKKVATAGADRTIRVFDVDTGKLLTQI
                     EDHADWIFGIAFSPDGKRLASASRDKTAKVFDIEKKESLVTFPGHAQPVYAISFTPDS
                     KGVATGGEDSRIRIWNPDGDGKSIRDVGGFGGTVFKVRYTPDGKNLLGCGADKAISVL
                     DAKGSPQSRLQGHNDWIYALTVSRDGKTVASGSWDGEVKLWNLADGKLVRTIVAAPGY
                     KPAGVQAAAAK"
BASE COUNT          586 a         1099 c         1053 g          710 t
ORIGIN      
        1 tatcggctca cgcccgccgc tgctctgacc gtcaccaaga gaacgcacct aagggcgacg
       61 acgcgatcac attccgcagt cgaacccgtt ctcacagttt tatcggcttc actcagattc
      121 gtagctgaaa cgattttacc aaaattaccc aatctagtta aaatcactag aatcggccta
      181 atcgttagat tccgcagttc tgagctgatt ggaggcacgg cgccggtttt tcgtaaacgg
      241 ccgcagtgtt cgttctcatt ctgtgacgcc gtcgaatttt cgccggaaac tgtcgccgcc
      301 tagttttctg cgttagtatg ctcgctatca acacgaaagg agaaccgacc gtgatcacca
      361 tgggtcaggg aaaatcggca acctgtttga gcgtgacacg ccgcgatttc cttcgtgtcg
      421 gaggggccgg gctgggctcg atcggtctgg ggctgactga cttcgacgcc cttgccggca
      481 tcgagcggca gccggatcgc tcggtgatct tgctcttgct agtcggtgga cccagccagt
      541 tggagacctg ggatcccaaa cccgacgcac cggcggaagt gcgcgggccg ttccgctcga
      601 tcgcgacccg ctgcccgggt gtgcgatttt gcgagcatct gccccgaatg gccgcgcgga
      661 tggatcggtt tgcggtcgtt cgttcggttc accacgactt ggcgccgatt cacgagacgg
      721 gctatcaact gctccagacc ggccggctct gccgggccgg cgagcaatac ccccacatcg
      781 gctcggtgat cgcgcggctg gacggggcga agaacgggtt acccgcggcc gcgatcctgc
      841 cgcgccccat tgccagcacg ggcgtcgaca tcccccaagg gcagtcggcg ggctggctgg
      901 gagccgccta cgatcccttc cacctgaatg ccgatccggc agcacccgag ttttgcgccg
      961 ccgcggccct cgatcgcgcc tgcactgcgt tcgaccgcac catgaacaat tctggatgtg
     1021 cgcaggtgcc agtacataac accctatcgc tcgtgcgccg gcacacccgc aaccccttcg
     1081 acattgccca agaggacgat agggtccgcg aagaatatgg tcgaacaagc ttcggtcaaa
     1141 gctgtctcct ggcccgtcgg ctggtcgagg cgggggtgcg gtttgtcacc gtcaatatgt
     1201 tcgacaccgt cttcaaccgc gccggctggg actgccacgg cgcggccccg ttcagcacct
     1261 tcggcgacta tgccagcttt gtgctaccga ccttcgacca gactttctcg gctctgatcg
     1321 acgacctcga ccggcgcggg cgactcgagt cgacgctggt gatcgcggct ggagaattcg
     1381 gccgcagccc ccgcatcaac gcgtcgggcg gccgcgacca ctggccgggc gtttggagcg
     1441 tcgttcttgc tggcggcggc gttcgcggcg gccaggtctt gggcgccagc gatgcccatg
     1501 ccaccgctcc ggtggaccgg cctgtaaccc cagaagacct ggccgtcacc atctaccaca
     1561 gcctgggcat cggcgaaaac gcgtccatta tcggacccga cggccagtcg cgccccgtcc
     1621 tcgaaaacgg gtctctgtta cgagaattgt tcgcatagca taggcgctcg ttccggcgtg
     1681 gagatgcgcc atcagtccgt atgcgaggcc gttacttcgc cgccgccgcc tgtactccgg
     1741 ccggcttgta ccccggcgcg gccacgatgg tccgaacgag cttgccgtcg gccagattcc
     1801 acagcttcac ctcgccgtcc cagcttcccg acgcgaccgt tttgccatcg cgtgagaccg
     1861 tcagggcata aatccagtca ttgtgtccct gcagtcggct ctgcggcgac cctttggcgt
     1921 cgaggacgga aatcgctttg tcggcaccgc atcccagcaa gtttttgccg tccggcgtat
     1981 accgcacctt gaacactgtg ccgccgaagc cgccgacgtc gcgtatcgac ttgccatcgc
     2041 catccgggtt ccagatccgg atgcggctgt cctcgccgcc ggtggcaacg cctttgctgt
     2101 cgggcgtgaa cgagatggcg taaaccggct gggcgtggcc cgggaacgtc accagcgact
     2161 ctttcttttc aatgtcaaaa accttggcgg tcttgtcccg gctggcgctg gccaggcgct
     2221 tgccgtcggg cgagaaggcg atcccaaaga tccaatcggc atggtcttcg atctgagtca
     2281 agagcttgcc ggtgtcgacg tcgaagaccc ggatggttcg gtcggcgccc gcggtggcga
     2341 ccttcttgct gtcggggctg aaggcgacgg cgaagacgac atcctgggtc tcggcgaggt
     2401 cgcgcaccgg cttgcccccg cctcccggct ccgccaacca gagcttggcg acgccataga
     2461 cgcccggatc gccgctggcg gtcgccagcc acttgccgtc cggactgtag gcaatgtcgt
     2521 acacccgctc ggccagcccg gttaacctgc ggtcgagcgt gccatcggat gtcttccaaa
     2581 atgtaatttc atgataaccc gatgccgcaa tagccgatcc atccgggctg aattcgagcg
     2641 ctgtgatggg cacggtcacg ggataagccg cgggaattgc aacctgctgc gttttgcgca
     2701 agacgatcgt ccagtcctcg ccaggcgaag tgccgtcgta cttggcccct tcggcgaccc
     2761 agcgcttgat gagcgcgatt ttttctgggg gcagggggtc ttgcttgaac ggcatcctgg
     2821 gctgggcgtc gggcaggagc acgtcgacca ggttgctctc ctcgggcttg cctggttcga
     2881 gagtcgtccc ttctccctgc tgcccccctt tggcaagctg cgcaaacgtc gtcatgacgt
     2941 acttactttc cgacttgcga gggttgtggc aggcgataca gttctcgacc aggacgggcg
     3001 cgacgtcgcg gataaagctg atcggctggt ccgcccgttt cggctcgatc tttacctccg
     3061 gctttgcctc ccttgacccg tcgttcttcg gttcagcccg agcaccgccc tctttgcctg
     3121 cggtggtgtt ggtctgaggt gccggcgtgt ctttcttgtc cttggtctga ccgtcgtcgc
     3181 cgagtgcaac ggtacacagt gataatgccg tggacagaaa cgccgaaaca acgaaaaaaa
     3241 ggtaccggga caacgaaggc ggagtggcgg atgcctttcc tttccccccc ttgtgaagcg
     3301 gggggccgtg gaggatagtc agggcgccat cggaatcagg tttttgggcg aattggccaa
     3361 ggacgaagct ctttcccacc cttgtgaaga gggggccagg gatagagtgc tttgtataag
     3421 tagaagtctg ataattcgac ggcattgg
//
LOCUS       DATAQL010001448         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3052003, whole genome shotgun sequence.
ACCESSION   DATAQL010001448 DATAQL010000000
VERSION     DATAQL010001448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3052003"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1854
                     /locus_tag="VHS97_25030"
     CDS             325..1854
                     /locus_tag="VHS97_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3451541.1"
                     /translation="MNMRWMISVGLAALFLGLPHRAFAAADQAQADAQESKIRESVVK
                     ISATMRYPDLTHPWTKHSPQEASGTGVVIDGKRILTNAHVVLYASQLFVESYQSSDKL
                     PATVESVSPGIDLAVLKLEDDAFFEKRPPIMRTSDLPEVKETVLVYGYPQGGSALSIT
                     KGIVSRIEFAGYNEGLSGVRVQIDAAINPGNSGGPALIDGKMVGLIFSKLTRTESIGY
                     IIPSEEIDLFLKDVADGTYDGKPAMHEALQTLENDALRSFLGLDRKTQGMVVHSSDPA
                     NPQDPLKPFDLLAKIGDNEIDNVGMVKVKDNLRLNFQYLIQKVVKDGKVPLTVVRQGK
                     SLAIDVPAKTKYPMLIESLKGKYPSYFIYGPLVFSPVTTEFIASFDRTGRLYSALALM
                     SSPLATRRGDLPKFEGEQLVVVTSPIFPNRIGKGYDNPLSKIVKEVNGVSIKNLRHFV
                     EVLRDSKEKFTTIVFNDKFSETIVFNHQDALKVTDEILSDNGIREQASEDLTAVWSKK
                     K"
     gene            1865..2707
                     /locus_tag="VHS97_25035"
     CDS             1865..2707
                     /locus_tag="VHS97_25035"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX3451542.1"
                     /translation="MNDLAVDRAARAIATADALLIGAGAGMGVDSGLPDFRGNDGFWR
                     AYPPYQRLGLDFVALASPHWFAEDPTLAWGFYGHRMGLYRQTSPHEGFSILRRWTSSA
                     REGSFVFTSNVDGQFQRAGFAPEAIVEVHGSFDGMQCIDECGVGIFAGGSFHVEIDPK
                     SMRAIHPLPSCPQCGALARPNILMFGDWAWESARTDLQMCRLNNWIESLDGARLVVIE
                     SGAGKAVPTVRITCENIARQKRGILVRINPREPDVPAGHVSLPMGALNALRALDARLN
                     ALAS"
BASE COUNT          590 a          806 c          807 g          569 t
ORIGIN      
        1 caatcgcgtt ttgtcgcact ggaaaataga gcgttgaatt tagtgccttc gaggccgagt
       61 tacaatgtat ggcctgcaac cagcctgttc aatcatcggc cactggtcgg tgccaaggac
      121 caaggacccc ggaccatgga cgattgagtc ttctctaccc acacgagaag ccaggggata
      181 agatgatgca caatctctgt cggctttgat cgcacgtttg ctgtcacgcc agattgcgcg
      241 acgagcgtgt caattccggc gagccgcggt ctggattggc ggcgattgaa tttcagcgtt
      301 cgataaccat aagcgagttc cgcgatgaat atgcgatgga tgatttcggt tgggctggca
      361 gccctgtttc tggggcttcc gcatcgggcc ttcgcggcgg cggaccaagc acaggcggac
      421 gctcaggaga gcaagatacg agagtcagtc gtgaagattt ccgcgaccat gcgctatccc
      481 gatcttactc atccgtggac caagcatagc ccacaagaag ccagcggcac cggagttgtc
      541 atcgacggca agcgcatcct gaccaacgcc cacgtggttc tgtatgccag ccagctcttc
      601 gtggaaagct accaatcgag tgacaaacta cccgcaacgg ttgagtcggt cagcccggga
      661 atcgacttgg ccgtactcaa gctcgaggac gatgcattct tcgaaaaacg gccgccgatc
      721 atgcgcacct cggatttgcc ggaggtcaag gagacggtgc tcgtttatgg ctatccccag
      781 gggggctcgg cgctttcaat caccaaggga atcgtgtcgc gcatcgaatt cgccggttac
      841 aacgaggggc tctcgggcgt gcgggtgcag atcgacgccg cgattaaccc cggcaacagt
      901 ggtgggccgg ccttgatcga cggcaaaatg gtcggcctca tcttcagcaa gctcacgcga
      961 acagagagta tcggctacat catccccagc gaggaaatcg acctgttctt gaaggacgtt
     1021 gcagacggga cctacgatgg caaaccggcg atgcacgaag cgctccagac acttgaaaac
     1081 gatgccttac ggtcctttct aggcctggac aggaaaaccc agggcatggt cgtccactcg
     1141 agcgatcccg ccaatccaca agaccccttg aagccgtttg acctgctcgc caagatcggc
     1201 gacaacgaga tcgacaacgt cggcatggtc aaggtcaaag ataatttacg tttgaatttt
     1261 caatatctga ttcagaaagt cgtcaaggat gggaaggtgc ccttgacggt tgtgcgtcaa
     1321 ggaaagtcgc tggcgatcga cgtgccggcc aaaacgaaat acccgatgct gatcgaatcg
     1381 ctcaaaggga aatacccttc atattttatt tacgggccct tggtattttc gccggtgaca
     1441 acggaattca tcgcgtcgtt cgaccgcact ggtcggctct actcggcgct cgcgctgatg
     1501 agcagccccc tggccacccg ccgcggcgac ctacccaagt ttgaaggtga acagttggtc
     1561 gtagtcacct cgccgatctt ccccaaccgg atcggcaagg gatacgacaa cccgctctcc
     1621 aagatcgtca aggaagtcaa cggtgtgtcg atcaagaacc ttcgtcactt cgtcgaggtg
     1681 ctgcgcgact cgaaggagaa attcacgacg atcgtcttca atgacaaatt ctcggaaacg
     1741 atcgtcttca atcaccagga tgcgctcaag gtcaccgacg agatcctttc agacaacggc
     1801 attcgcgagc aagcttcgga ggatctgacg gcagtctggt ccaaaaagaa gtgagtcctg
     1861 attgatgaat gacctcgccg tggaccgggc ggctcgagcg atcgccaccg ccgacgcctt
     1921 gttgatcggc gccggcgcgg gcatgggagt cgacagcgga ctgcccgatt tccgcggcaa
     1981 tgacggcttt tggcgcgcct atccgcctta tcagcggctt ggattggatt ttgtcgcgct
     2041 ggccagtccg cactggttcg cggaagatcc cacgctcgcc tggggtttct acgggcaccg
     2101 catggggctg taccgccaaa cgtctcccca cgaagggttc tcgattctgc gccgctggac
     2161 cagctcggct cgcgaaggca gtttcgtttt cacgtccaat gtcgacggcc aatttcagcg
     2221 cgccggcttt gcccccgaag cgatcgtcga ggttcacggc tcgtttgatg gaatgcagtg
     2281 catagacgag tgtggcgtgg gcatctttgc gggcggatcg ttccatgtcg aaatcgatcc
     2341 caagagcatg agggcgattc acccattgcc atcgtgcccg caatgtggtg ccctggcccg
     2401 gcccaatatt cttatgttcg gcgactgggc gtgggagtcg gcccggaccg atctccagat
     2461 gtgccggctg aataactgga ttgagtcgct cgacggcgcg cgcttggtcg tgatcgaatc
     2521 tggcgccggc aaggcggtcc cgaccgtgcg catcacctgc gagaacatcg cccgccaaaa
     2581 gcgcggcatt ctggtgcgga ttaatccgcg cgagcccgac gtgcctgccg gccatgtttc
     2641 cctgccgatg ggagccttga atgcgctccg tgccctcgac gcgcggttga acgcactcgc
     2701 ctcttgaaca tatctacagc gcaaagccgg acaaggtcag cgggttcccc gagcgtcagg
     2761 tcaagacttc cg
//
LOCUS       DATAQL010001449         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_761631, whole genome shotgun sequence.
ACCESSION   DATAQL010001449 DATAQL010000000
VERSION     DATAQL010001449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761631"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VHS97_25040"
     CDS             <1..185
                     /locus_tag="VHS97_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451543.1"
                     /translation="DRMSWDIEQHGKDEAWFNFSIFADDNSWMAGVELATASERPARK
                     LRIWGRRASGVDPSHP"
     gene            434..856
                     /locus_tag="VHS97_25045"
     CDS             434..856
                     /locus_tag="VHS97_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX3451544.1"
                     /translation="MLLIAHGSRRQSANEDLHGLAARLTAQGTYPIVEACFLELSEPD
                     IPTGGHKCVSRGASRVLLIPYFLSAGVHLRRDLTAARDELSRLHPSVDFLLGAALGPH
                     PLLDELVAKRIRELDPRDPHNAAAIRPLSSIDTNLANQ"
     gene            953..2134
                     /locus_tag="VHS97_25050"
     CDS             953..2134
                     /locus_tag="VHS97_25050"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX3451545.1"
                     /translation="MTVPLQSLAVPALNLRAQYQTIRDEIEPVVLRLFESQMFVLGPE
                     VEKLEAELAEYCGVARGIGCASGTDALLLPLMARAIGAGDEVITTPYTFFATAGSIWR
                     TGAKPVFVDIEPDTYNIDPTRIEAAITPQTRAIIPVHLYGQTADMDPILEIARKHDLF
                     VIEDAAQAIGAIYKGRCAGSLGDVSALSFYPSKNLGGFGDGGMMTTDDPVLARSLARL
                     RVHGMEPKYHHHEVGFNSRLDALQAAVLRVKLSHLDDWTLKRREVASRYRGLFEAHGL
                     TKAVGLPREREGHYHVFNQYIIRVPAPVRDPLRDYLTSRQVGTEIYYPIPLHLQPCFA
                     ELGHKPGDFAVAEAAANETLALPIYPELSDEQQRYVVGTIRQFLDSHAHAHLISADRA
                     A"
     gene            2666..>2968
                     /locus_tag="VHS97_25055"
     CDS             2666..>2968
                     /locus_tag="VHS97_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451546.1"
                     /translation="MTRRQESMGSRLGCGENEAEEPGSPVRYEGSALDQDEGWVSGMQ
                     ARLMAWYTEARRDLPWRATRDPYRILVSEMMLVQTTVTAVIPYFERFLNQFPDAGSL"
BASE COUNT          585 a          891 c          874 g          618 t
ORIGIN      
        1 ttgaccgtat gtcatgggat atcgaacagc acggaaagga tgaggcgtgg ttcaacttta
       61 gcatcttcgc agatgacaac agctggatgg ctggagtcga gcttgcgaca gccagcgagc
      121 gcccggcgcg gaagcttcgg atctgggggc gtcgcgcttc gggcgtcgac cccagccacc
      181 cctgaggtgt aacctgcgtt tgtacctcgc caaactttgt ttccaaacgg tgtcgcaggc
      241 catggaagag caagggggct gggagacgat cgccctggca ggtccggtgg ggccgattca
      301 accctcgcat tgacccacgg cgtttggcac ctacgatatt gggtgtcgag cgagactccg
      361 cctgcgggtt gggtagaaca tggaacaatc cggcagaaat gctggcatat cgtcatgctc
      421 tgactgcacg gcggtgctac tgatcgctca tggcagccgg cggcagtcgg caaacgaaga
      481 cctacatggt ctggccgcgc gattgacggc tcagggaacc taccccatcg tcgaagcctg
      541 ttttctcgag ctctccgagc ccgacattcc gacaggcggc cacaaatgcg tgtcgcgcgg
      601 cgcaagtcgt gtgctgctga tcccttactt cctctccgcc ggcgttcacc tgcgccgcga
      661 cctgactgcg gcgcgggatg aattaagccg tctccatccc tcagtcgact tcctcttagg
      721 tgcagccttg gggccgcatc ctttactcga tgaactcgtc gccaagcgga tccgcgagct
      781 cgacccgcgc gacccgcaca acgcggcggc tattcggcct ttaagctcca ttgacacaaa
      841 tcttgcaaac caatagcgtg cggcgtccga tatctccatg gagggagatg gtgcgcatcg
      901 atgggtgaac gacccttcgc actcgcatga ggcgtcaaag gagtgatcgc cgatgactgt
      961 tccgctgcaa tctctggccg tgccggccct taacctccgc gcgcaatacc agacgatccg
     1021 cgatgagatc gaacccgtgg tcctccggct ctttgaaagc cagatgtttg tactggggcc
     1081 cgaggttgaa aagctcgaag ccgagcttgc cgaatattgc ggcgtggctc gaggaatcgg
     1141 ctgtgcatcg ggaaccgacg cgctcctctt gcctctgatg gcacgagcga tcggcgctgg
     1201 tgacgaggtg atcacgactc cctacacatt ctttgccact gctgggtcca tctggcgcac
     1261 cggcgccaag ccggtctttg tcgacatcga gcctgatacc tacaacatcg atcccacgcg
     1321 gatcgaagct gccatcaccc cgcaaacacg ggcgatcatc ccggtacatc tttacggcca
     1381 aactgccgat atggacccga ttctcgagat cgcgcgaaag catgacctgt tcgtgattga
     1441 agacgccgct caggccatcg gagcgattta taagggccgc tgcgcgggca gcctgggtga
     1501 tgtgtcggcg ctgagctttt atccgtccaa aaacctgggg ggcttcggcg acggcggcat
     1561 gatgacgact gacgacccgg tgctggcccg ttcgctggca aggctgaggg ttcacggaat
     1621 ggagcccaag tatcatcacc acgaggttgg cttcaactcg cggctcgacg cgctccaggc
     1681 ggctgtctta cgcgtaaagc tttcccacct cgacgactgg actctcaaga ggcgcgaggt
     1741 cgcgtcgcgg tatcgaggtt tattcgaggc ccacggactg accaaggcag tcggcttgcc
     1801 gcgcgaacgg gaaggccatt accacgtctt caaccagtac attatccgcg tgccggcgcc
     1861 cgtccgcgac ccgctgcgcg actatctaac ttcacgtcag gtcggcaccg agatctacta
     1921 tcctataccg ttgcacttgc aaccttgctt cgccgagctg ggccacaaac ccggtgattt
     1981 cgcggtcgcc gaagcggctg cgaatgagac tctggcattg ccgatctatc ccgagctgag
     2041 cgatgagcag cagcgttacg tcgtgggaac gattcgtcag ttcctcgatt ctcacgccca
     2101 tgcccacctg atctcggcgg accgggcggc ctgagggtcc gacgggccgg aacgaagctc
     2161 gaacggcgat cggcaaggtc gccgtcgact tcgagcttcg tctcaattgg tcgattctag
     2221 ctcctcgctc tcggccggcc agatggcgca caatcgctgg gcaagatggt gcgccatcgg
     2281 catgtatcgg actgcaagcc cgcgctgatc ctcgttcaac gggattcgat cagggcaggg
     2341 gacgctactt tcggcgtcgg tgctgtcgta ttcttgagtc tctggcgacg ttttgtttcc
     2401 cggtgcctca agctcccttg aattcgcttt gtcggcaagg gacacccgcc aagcggacaa
     2461 cggccctcga ccacgcctcg attcgctatc cacgagcacc cccatggact gtttttaata
     2521 aatgtctctg tcgtaaaaaa aagcgatttc cttggagatt gtgtcccttc tatcactcat
     2581 tcaacctgcg ttatcgttct ggattgggta cgcaccatag aaatcctaaa aacggttcat
     2641 gaatttgcga aaagaggaat gcgacttgac gcgacggcag gaaagcatgg gttctcggct
     2701 cggttgcggt gaaaacgagg cagaggagcc tggctcacca gttagatacg agggatcggc
     2761 tctcgatcag gacgagggct gggtcagtgg catgcaagcg cggctaatgg cttggtatac
     2821 agaggcccgg cgggacttac cctggcgggc cacccgcgac ccgtaccgca tcctggtatc
     2881 ggagatgatg ctggtccaaa ccactgtgac ggcggtcatt ccgtactttg aaagattcct
     2941 caatcaattt ccggatgccg gctcactg
//
LOCUS       DATAQL010001450         5829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3815129, whole genome shotgun sequence.
ACCESSION   DATAQL010001450 DATAQL010000000
VERSION     DATAQL010001450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3815129"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VHS97_25060"
     CDS             <1..664
                     /locus_tag="VHS97_25060"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX3451547.1"
                     /translation="VFQTKIALFQCTSDRSNDFVPAAPFNTATRGNYVINWGNTQYDQ
                     GLITTNLSSAKPPIVTAPMPFPLDKAVSFAAFTDGLSNSLLMSECLQGSGMDLRGYIW
                     TSLPGAGGFYTRFTPNGFTDFYQLPVPTRQAGWPASGIANADITDENFCNPEPAAGMP
                     CLVVSTFAYAYAAARSRHPGGVNALFADGSVRFIKNSINGATWIALGSIGAGEVISSD
                     SY"
     gene            complement(757..2079)
                     /locus_tag="VHS97_25065"
     CDS             complement(757..2079)
                     /locus_tag="VHS97_25065"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HEX3451548.1"
                     /translation="MRPVALTGLLICSLAATVRAAGDDADRFLETAKKLIQAINRHDS
                     LAIQASLDAQMQQVLPPDKATPFFRGLVAAKGKLKEAGAPQVTGPTAIVRVTAERGAW
                     DFKISLRPSGKIAGLLITEAAAEAPAPASAQRFTKMANKLIQAINSNDSAAIQASLDT
                     QMRRAFPPDKTTSFFGGLVSAAGKLKDAGVPQVTGPTAIVRVTAERGAWDFKITLDAS
                     DKISGLSVTPPGSAPAADSTAVPRSRTPMQLPFRGEWYVFGGGDNEKVNDHVSALGQR
                     RAADILIKGADDRSHKGTGRRNEDYFVYGKEILAAAPGTVVTAIDGVPDNEPGSMNPL
                     CAVGNCLIIDQGSNEYAVYAHLKPGSLRVHRGDKVRRGQVLALCGNSGNSSEPHLHFH
                     LQDNAILQDGAGITPYFTNVKCRRGDATLTDAEYTFLQGDHIQSATKK"
     gene            2304..2708
                     /locus_tag="VHS97_25070"
     CDS             2304..2708
                     /locus_tag="VHS97_25070"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3451549.1"
                     /translation="MKETSGHSHFTPDGWHTVTPRIVVNDAERLVAFLREVFGATGAY
                     RPDLPSEMKIGDSIVMVSDAGIRKPMPAFLYVYVHNTDDTYRLALQAGASSLEEPSDM
                     PYGDRRGMVQDEWGNTWQIATHLLMSRPAGQR"
     gene            complement(2860..4677)
                     /locus_tag="VHS97_25075"
     CDS             complement(2860..4677)
                     /locus_tag="VHS97_25075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451550.1"
                     /translation="MSRPVFVLATDLRREEAAWASMEAQAASLQAFAERTLGMSAWVD
                     LACLPARSTEDPGEYQIGRVIDRRVGDGTSLLFVMPAAFDQNIWQRTMLGEELGAARR
                     RHAGASIHHDSVEPTHSLLVDCFAGQILRALDERGVEPRNAGVVLVADGQGDPATRAD
                     SYRLMRLVWEQAGLGRGDVGFVRHPQPFLPETLERCLSEPLEWVLQPQRQWDGELCDF
                     ARVMLDDHRRSHPEAAGWRLLDPPREHPAIQAWLEQRMLRLWQEKRARQAARIPSARN
                     ELPPARAGVWSGADWVSAAEAPSRPRAGYVARARDSAALAEVLARVLPPAERYLVKVT
                     WHGYAPGTYTGPLALDLLLGALPARAVLLEGHTSSRNLGGTELDWDADVKRHRIWIRQ
                     QDVEFLRRMGLAEVIARHGAQYLNVTEAWWDGACAPAEDVRAVLGKVALRNPELAGFV
                     PSALMELRGIPLISFARFKGPTRLGISNLFGLIPRPLRTEWHGPDITHFASVCCDLAR
                     LYGSFFPLFGVVEAFDSAVRWDRNGLYRSRWGNYDLVLTDGLFTVSEGLVGADLLASR
                     LQGQDVLRSGFYDVVRERLGWSAAEAEIALPGDLQAILV"
     gene            complement(4689..5768)
                     /locus_tag="VHS97_25080"
     CDS             complement(4689..5768)
                     /locus_tag="VHS97_25080"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX3451551.1"
                     /translation="MKDQRDEAAIPSAYAEKEKKRIKELGLEPADSIEDKSDISCFQR
                     GSSPHWSGINTFLKLPYVEDVRKVGEYDVAFVGAPFDIGCTFRTDTRFGPQGMRRISS
                     LYQKYSYEHGIDLGETLAMCDVGDIFCPSNITKGHDQITKGVAHILSQGTLPMIMGGD
                     HSIGYPIARGVAQCVEGNVGIIHVDRHVDTQDKDMDEVMHTCPWFHATNIPNCPPKNL
                     VQLGIGGWQVPRAGVKVGRERGTTILTVNDIEKIGIDKTIEIALEVAWKGASAVYMSF
                     DIDSLDAGFVPGTGWPEPGGLLPREALKLVNGIAKEGIAAMEVCEVSPPYDISDVTSL
                     MGVRLMMDVLGTMAKNRRIGGRLKI"
BASE COUNT         1062 a         1841 c         1835 g         1091 t
ORIGIN      
        1 tgtctttcag accaagattg ctcttttcca atgcacaagc gatcggtcaa acgacttcgt
       61 ccccgcggca cccttcaaca ccgcgactcg gggaaattat gtgatcaact ggggcaacac
      121 tcaatatgac cagggattga tcacgaccaa cctctcgagc gcgaagccgc cgatcgtcac
      181 tgcgcccatg ccctttccgc tcgacaaggc cgtctcattc gcggcattca ctgatggatt
      241 aagtaattct ttgctcatgt cagaatgtct acagggttca gggatggacc ttcggggcta
      301 tatctggacc agcctgccag gggcgggcgg cttttacacc cgattcacgc ccaacggctt
      361 caccgatttt tatcaactgc cggtccccac gcggcaggcc ggctggccag cgagcgggat
      421 cgccaatgcc gacatcacgg acgaaaactt ttgtaacccg gagccggctg ctgggatgcc
      481 gtgtctggta gtcagcacgt tcgcctatgc atatgccgcc gcccgaagcc gtcaccccgg
      541 aggcgtcaac gcactcttcg ccgacggatc cgtgcgattc attaagaatt cgatcaacgg
      601 tgccacttgg atcgcactgg gatcgattgg cgccggcgag gtcatcagct ccgactcata
      661 ctgagcggca gtacgggaat tacctgacct tggcagaatc cgttggcgca ccctttctcc
      721 acccgcgagc gagagaccag ggtgatcagg actgggttac ttcttggttg cggactgaat
      781 gtggtcgcct tggaggaagg tgtattcggc atcagttagg gtggcatcgc cgcggcggca
      841 cttgacgttg gtgaaatagg gagtgatgcc ggcgccgtcc tgaaggatgg cgttatcctg
      901 cagatgaaaa tggaggtggg gttcgctgga attgccgctg ttgccgcaga gggcgagcac
      961 ttggcccctg cggaccttgt cgccgcggtg gacgcgcagg gacccgggct tgagatgcgc
     1021 gtagacagcg tactcgttgg agccttggtc aatgatcagg cagttgccga cggcgcacaa
     1081 agggttcatg gagcctggct cgttatcggg gacgccgtcg atcgcggtga cgacagtgcc
     1141 gggggctgcg gccaggattt ccttcccgta aacaaagtaa tcttcgttgc ggcgaccggt
     1201 acctttgtgc gagcggtcgt cggcaccttt gataagtata tctgcggcgc ggcgctggcc
     1261 aagcgcggag acatggtcgt tgaccttttc gttgtcgccg cccccaaaga cgtaccactc
     1321 gcctcgaaac ggaagctgca tgggggtgcg gctgcgcggg acggccgtcg agtcggccgc
     1381 gggagcggaa ccgggcgggg tcacggataa accggaaatc ttgtcggacg cgtcgagggt
     1441 gattttgaaa tcccacgctc cgcgctcggc cgtcactcgc acgatggcgg tgggaccggt
     1501 gacttgaggg acgccggcgt ctttcaactt gccggcggcg gacacaagtc cgccgaaaaa
     1561 ggaggtggtc ttgtcgggcg gaaaggcccg ccgcatctga gtgtcgaggc ttgcctggat
     1621 ggcggccgaa tcgttgctat tgatcgcctg aatgagtttg ttggccatct tggtgaagcg
     1681 ctgggcggaa gcgggcgcgg gcgcttccgc agcggcctcg gtgatgagca gtccggcgat
     1741 cttgcctgag ggtctcaggc tgattttgaa gtcccaggct ccgcgctcgg ccgtcactcg
     1801 cacgatggcg gtgggaccgg tcacttgcgg cgcgccggct tcctttaact tgcctttggc
     1861 ggccacaagt ccgcggaaaa aaggagtggc cttgtcgggc ggcaagacct gctgcatctg
     1921 agcgtcgagg cttgcctgga tggcgagcga gtcatggcgg ttgatggctt ggatcaattt
     1981 cttcgcggtt tcgaggaagc ggtcggcgtc gtcgccggcg gcgcggacag tggcagccaa
     2041 tgagcaaatc agcaagccag ttagcgcgac gggtcgcata aaaggagtcc ttgtcggttt
     2101 gaggccgaaa tgtagagaat cgaaggcatt tgagttagtg taacgagagg gcttcagggc
     2161 cgacagagac aggaattcga gttggggctt ggttattcgt ccggcggcac aaatctgccg
     2221 cgacccatgg ccagcttaag gctttacggc cgatgagata gcgattgcga tcaggccgcc
     2281 ggtgcaaatc gagggaatac gcgatgaaag aaacgagtgg ccattcgcac ttcacccccg
     2341 atggctggca tacggtgact ccacgaatcg tggttaacga cgcagaacgg ctggtcgcgt
     2401 ttctccgaga ggtgttcggg gcaacagggg cctatcggcc agatctacca tccgagatga
     2461 agatcggcga ctcgatagta atggttagcg acgccggaat ccgcaaaccg atgcccgcct
     2521 ttctgtacgt ctacgtccac aacaccgatg acacgtacag gctggctctc caagctggtg
     2581 caagctccct ggaagagcca tcggacatgc cctatggcga ccgtcgcggc atggttcagg
     2641 atgaatgggg taacacgtgg caaatcgcca ctcacctctt gatgtcgcga cctgctgggc
     2701 agcggtagct ctcctccggc gcaagcgaaa tgcacggtcg accgcgccac atcctttaca
     2761 tggaattctt caccgacgcc agcacatgtg gccgcgtcgg aaagtccatg gtgacacctc
     2821 cacgcagctc gtaagatcga agcgcgggtg cttcttactt catacaagta tcgcttggag
     2881 atcgccgggg agtgcgatct ccgcctccgc cgccgaccag cccagtcgct cgcggaccac
     2941 gtcatagaac ccgctacgca atacgtcctg gccctgcagt cggctcgcca ggaggtcagc
     3001 ccccaccagc ccttcgctca cggtgaatag cccgtcggtg aggaccaggt cgtaatttcc
     3061 ccaacggctc cggtacagcc cattgcgatc ccagcgcacg gccgagtcga aggcctccac
     3121 gacaccgaac aggggaaaga aagacccgta gagccgcgcc aggtcgcagc agaccgacgc
     3181 gaagtgggtg atatccggcc catgccattc tgttcgcaaa gggcgcggga tcaagccgaa
     3241 gaggttcgag atgccgagtc gggtcggccc cttgaagcgg gcgaagctga tcaacggaat
     3301 cccgcgaagc tccatcaagg ccgagggaac gaaaccggcc agctccggat tccggagcgc
     3361 gaccttcccg agcacggccc ggacgtcctc ggcaggcgca caggccccgt cccaccacgc
     3421 ctcggtcaca ttcagatact gggccccgtg ccgggcgatc acctccgcca accccatccg
     3481 ccgcaggaat tcgacgtctt gctgccggat ccagatgcgg tgacgcttga cgtcggcgtc
     3541 ccagtcgagc tccgtaccac caagattccg gctgctcgta tgcccctcca gcaaaaccgc
     3601 ccgcgcgggc agtgcgccga gcaggaggtc gagcgccagc ggccccgtgt aggttcccgg
     3661 cgcatagccg tgccaggtca ccttcaccag gtagcgttcg gccggcggga gtacccttgc
     3721 caggacctcg gccagcgcgg cggagtctcg ggcccgcgcg acatacccgg cccgcggtcg
     3781 gctcggcgct tcggccgccg acacccaatc cgcgccggac cacaccccgg cccgcgccgg
     3841 cgggagctcg ttcctggcgg agggaatccg cgccgcctgg cgggctcgct tctcttgcca
     3901 gagccggagc atccgttgtt cgagccaggc ctggatcgcc gggtgttccc gcggcgggtc
     3961 aagcagccgc cagcccgccg cctcggggtg ggaccgtcgg tgatcgtcga gcatcacccg
     4021 ggcgaagtcg cacagttcgc cgtcccactg gcgctgcggc tgcaacaccc actccagcgg
     4081 ctcgctcagg caccgttcca acgtctcggg gaggaatggc tgcgggtggc ggacgaagcc
     4141 gacatctccc cggcccaggc cggcttgctc ccagaccaac cgcatcaggc gataagagtc
     4201 ggcgcgggtg gccggatcgc cctgcccgtc tgcgacgagg acaactccgg cgtttcgcgg
     4261 ctcgacaccc cgctcgtcca gcgcccgcaa gatctggccg gcaaagcaat cgacgagcag
     4321 cgagtgcgtg ggctcgacgg aatcatgatg gatcgaggcc ccggcgtgcc gacgccgcgc
     4381 cgcgcccaac tcctcgccta gcatcgtccg ctgccagata ttttgatcga atgcggccgg
     4441 catcacgaaa agtaatgagg tcccgtcgcc gacgcgtcga tcgatcaccc ggccgatctg
     4501 gtactctccc gggtcttccg tcgatcgggc tgggaggcag gcgagatcga cccacgctga
     4561 catcccgagg gtgcgctccg cgaacgcctg aaggctcgct gcctgggcct ccattgacgc
     4621 ccaggcagct tcctcccgtc gcagatcggt cgcgagtaca aagacgggtc tcgacaccgc
     4681 cgatccaatc agatcttgag ccggccgccg atccggcggt tcttggccat cgtgcccagc
     4741 acgtccatca tcagccgcac acccatcagc gacgtgacgt ccgagatgtc gtacggcggc
     4801 gacacctcac agacctccat ggcggcgatc ccttctttcg cgatgccgtt gacgagcttg
     4861 agcgcctcgc gggggagcag tccgccgggc tcgggccagc cagtgccggg gacgaatccc
     4921 gcatcgagcg agtcgatgtc gaacgacata tagaccgcgc tcgccccctt ccacgcgact
     4981 tcaagggcga tctcgatcgt cttgtcgatc ccgatcttct cgatgtcgtt gacggtcagg
     5041 atcgtcgtgc cgcgctcccg ccccaccttc acgccggcgc ggggtacctg ccagcctccg
     5101 atccccaact gcaccaggtt cttcggcggg cagttcggga tgttcgttgc gtggaaccat
     5161 ggacatgtat gcatgacttc gtccatatcc ttgtcctgcg tgtccacgtg acggtcgacg
     5221 tggatgatgc ccacgttccc ttccacgcac tgggccacgc cgcgggcgat gggatagccg
     5281 atggagtggt cgccgcccat gatcatcggc agggttccct gcgacaggat gtgcgcgacc
     5341 cccttggtga tctggtcgtg gcccttggtg atgttggacg ggcagaagat gtcgccgacg
     5401 tcgcacatgg cgagcgtctc acccaggtcg atcccgtgct cgtagctgta tttctggtag
     5461 agcgaggaga tccgtcgcat cccttgcggc ccaaacctgg tatcggtccg gaacgtgcag
     5521 ccgatgtcga acggcgctcc cacaaatgcg acgtcgtatt cgcccacctt gcggacgtcc
     5581 tcgacgtaag gcaacttcag gaaggtgttg atgcccgacc aatgcggcga gcttcctcgc
     5641 tggaagcaac tgatgtcgct cttgtcctcg atagagtcgg ctggctcaag tcccagctct
     5701 ttaatccgct tcttctcctt ctcggcgtag gccgagggaa tggcggcctc gtcacgctga
     5761 tccttcatcc cctcgtactg gctcaggtcc ggaaggtttc gagcatgctg ccgatggttc
     5821 ggagcgtgc
//
LOCUS       DATAQL010001451         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2592448, whole genome shotgun sequence.
ACCESSION   DATAQL010001451 DATAQL010000000
VERSION     DATAQL010001451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2592448"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..>1838
                     /locus_tag="VHS97_25085"
     CDS             388..>1838
                     /locus_tag="VHS97_25085"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX3451552.1"
                     /translation="MAAILLALSVGLCAGYLDVGVIVFRKLFWNQEGYFRTGRDFLWT
                     VPVGHALLLLIPGLAVVAVNRMRPGLISVRGGLWLYATLGISGALLRIPLHIAANLVL
                     AAGLGRLASDVVTARWLQPRPARSILAALIGLLFALAALSSGRQALGEYRAVAGLSPA
                     PPGARNVVLIVWDTVRSYNLSLHGYPRDTTPNLTKWALEGVRYDRALAPAPWTYPSHS
                     SFFTGQWPFKLNTQWNFRLDTPDSTLAEYLTSRGYQTAGFSANTNHCSYETGLARGFT
                     HFEDYALVTRSVLSRTVPGNWILKNILSRGNFYAEKWIGLQSLGARELNQSFLDWLSH
                     RRPDRPFFAFLNYYDAHDPYIPPAEFEGRFGIRPKTARDYQLLFSFEQPHELPTTKRD
                     ILMARDCYDDCIAFLDEQLGRLLDELKRRGLLDTTEVIITSDHGESFGDHGVFGHSAS
                     VDLDEIGVPLVILSPAAPAGQVVDSPVSLCDLPA"
BASE COUNT          304 a          606 c          540 g          388 t
ORIGIN      
        1 tgatcttgtc gatgatttac cgggacagtc gtcaccctat catacatgat cactgggaag
       61 aacgaacgcg tcggtcgcac acgcttcgag tcgaaaggtt gttcctggcc gatccactct
      121 ggaaacgaga gccgacaagg gatctggcca gaaccactcg aaacgtctcg ggcaacatga
      181 taccgtaaaa agcaggcttt ggtcggggcg tctcggtttt cgccgaacgc gcggttcggc
      241 gttgttcgtc gcccgccggc gtaatcgcat ggctttgctc taggcagcga tcgcccacaa
      301 gattaacgcg cagcgtaggc ctcgatggac accaccccgc ccccaacagc gtctcggcat
      361 ctgccgagcc agattccgct gtccccaatg gctgcgattc tgctggcgct ctccgttggc
      421 ctctgcgcgg gctacctcga tgtcggcgtc attgttttta gaaagctctt ttggaaccag
      481 gagggttatt tccgtaccgg gcgggatttc ctctggaccg tcccggtggg ccacgcgctt
      541 ttgctgctga ttccggggtt ggcagttgtg gccgtgaacc gaatgcggcc gggactcatc
      601 tcggtccgcg gaggcttgtg gctgtacgca acgctcggga tctccggggc actcttgcgg
      661 attcccctgc acatcgcggc caacctggtc ctggccgccg ggctgggtcg gctagccagc
      721 gacgtggtca cggcccgttg gctgcaaccg cggcccgcgc gctctatcct ggcggcgctt
      781 atcgggctgc tctttgccct ggcggcccta tcttccggcc gacaggcgct cggcgagtac
      841 cgcgcggtgg ctggattgtc ccccgccccc ccgggcgccc gtaacgttgt gctgatcgtc
      901 tgggataccg ttcgttccta caacctgagc ctccatggct atccccgcga caccaccccc
      961 aacctgacaa agtgggccct tgagggcgtc agatacgacc gcgcccttgc gccagcgccg
     1021 tggacttacc cctcgcatag ttcgttcttc accggccagt ggccgttcaa gctcaatact
     1081 cagtggaatt tccggctcga caccccggat tcgaccctgg ccgagtacct gacatcacgc
     1141 ggctatcaga ccgccgggtt ctcggcgaac accaatcact gcagctacga gaccgggctg
     1201 gcccggggtt tcacccattt cgaggattac gcgctcgtga cgcggtctgt gctcagtcgc
     1261 acggttcccg gcaactggat cctcaagaac attctgagtc gcggcaactt ctacgccgag
     1321 aagtggatcg gtcttcaatc gctgggcgcg cgcgagctca accagtcgtt tcttgactgg
     1381 ctgagccacc ggcggccgga tcgtcccttt ttcgccttct tgaactacta cgatgcccac
     1441 gacccgtaca tcccgccggc ggaatttgaa ggccgcttcg gaattcggcc caagaccgcg
     1501 cgcgactacc agctcctttt cagttttgaa caaccgcacg agctaccgac gacgaagcgg
     1561 gacatcctga tggctcgcga ctgctacgac gattgcatcg cgttcctcga tgaacagctc
     1621 ggtcgtttgc tcgacgagtt aaagcgccgg gggctgctcg acaccacgga ggtgatcatc
     1681 acctcggacc atggggaatc gttcggcgat cacggtgtgt tcggccactc ggccagcgtc
     1741 gacttggacg agatcggcgt tccgctggtg atcctctccc cggctgcgcc tgcgggccag
     1801 gttgttgaca gccctgtcag cctctgcgac ctgccggc
//
LOCUS       DATAQL010001452         3540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_609535, whole genome shotgun sequence.
ACCESSION   DATAQL010001452 DATAQL010000000
VERSION     DATAQL010001452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609535"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..2301
                     /gene="uvrB"
                     /locus_tag="VHS97_25090"
     CDS             241..2301
                     /gene="uvrB"
                     /locus_tag="VHS97_25090"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX3451553.1"
                     /translation="MPAFELVNPFQPAGDQPQAIEQLVDGLRQGRSHQTLMGVTGSGK
                     TFTMANVIAQYGRPALVMSHNKTLAAQLYAEFKEFFPRNAVRYFVSYYDYYQPEAYIP
                     QRDIYIEKDAAINQEIERLRLACTSALVSREDVLVVASVSCIYGLGSPEDYRKMMVRL
                     QLGDMIDRDKLLLKFVDIQYDRNDVSFERGKFRVRGDVVELWPAYEEFGYRIELFGDE
                     VERLATIDPLTGSVLDTHKDMYIYPAKHFVLPEERIQGAVENIGNELEGRLKDLKEQG
                     KLLEAQRLAARTRYDMEMLMEVGYCSGIENYSRHLSNRKPGETPTTLLDFFPPDSLLI
                     MDESHVTVPQVRGMFAGDFSRKSTLVDHGFRLASALDNRPLRFDEWEKKFDRRLFVSA
                     TPSDYEVAMSGGEVVEQVIRPTGLIDPIVRVEPARGQVPALLKEAKERAARGERVLIT
                     TLTKRLAEDLSRYLKEQGLRCKWLHSELDAFERVTILRELREGAFDALVGVNLLREGL
                     DLPEVSMVCILDADKEGFLRSETSLIQTIGRSARHVNAEVVLYADKITPSMRRAIDET
                     ERRRALQLEYNKEHGITPETIVKAIRRGIEEEIQARTIVRRAVGRDETTDATEEYLSA
                     LEAEMLDAAEKLEFERAAALRDKILELRSGNDDGPRRPAPSPQAQSARTKARAKARGG
                     RRKQ"
     gene            2451..3395
                     /locus_tag="VHS97_25095"
     CDS             2451..3395
                     /locus_tag="VHS97_25095"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3451554.1"
                     /translation="MRRWFCFLDKDKMPTHRNPVRLAGIPALGALLLFTAVVSIGCDS
                     NSFVPPRPEELRGTGSTTLAPLKNASSDPAGLDFASARAVEIVLAPRDGDEAEVWKSA
                     ARTQSGHEKIKVKVTIAAADQPTSKQMELIREAMARHPRVLVVEPADPSDSALAQAVA
                     ETRAQGIPVVLLGRPLTGDNKTPATASTSLTGPLVVVAPPPFSSSARELVAAAIRNAN
                     AAELDPNGGAIIVINTLGDQFIPDRVAGIRDALKTAGITSVEEIRFEGDVTKGENLVK
                     DGLKAHPKATLIFTVDYLSTSAMRGLLNATANSPTFVA"
BASE COUNT          775 a         1021 c         1095 g          649 t
ORIGIN      
        1 agatccatgg cccagacggc agtccaagga caaaggactg aggattgagg actgagaact
       61 gaggactgag gactgaggac tgaggactga ggactgagga ctgaggactg aggactgagg
      121 actgaggact gaggacggtg caccaatgac caatgactga tgaccaatga ccaaattcgg
      181 taaaataata gagaagttga gaaagggacc tatctatccc gcaccaaggt aggacacttc
      241 atgcctgcat ttgagctcgt caatccattt cagccggccg gcgatcagcc ccaagcgatc
      301 gaacagctgg tcgatggtct gcgccagggc cgttcccacc agacattgat gggggttacc
      361 gggtctggca agacgttcac gatggccaac gtgatcgccc agtacggtcg ccccgccttg
      421 gtcatgtcgc acaataaaac actcgccgcc cagctttatg cggagttcaa ggaatttttc
      481 cccagaaacg cggtccgcta cttcgtcagc tattacgact actatcagcc cgaggcctac
      541 attccccagc gcgacattta tatcgagaaa gacgctgcca tcaatcaaga gatcgagcgg
      601 ctgcgcctgg cctgcaccag tgcgctcgtc agccgcgaag acgtcctggt cgtggccagc
      661 gtctcgtgca tctacggctt gggctcgccg gaagattatc gcaagatgat ggtccggcta
      721 cagttaggtg acatgatcga ccgcgacaaa ttgctcctca agtttgtcga tattcagtat
      781 gatcgcaatg acgtgtcgtt tgagcgcggt aaattccgcg tgcgcggcga tgtggtcgag
      841 ctgtggccgg cctacgaaga gttcggctat cggatcgagc tatttggcga tgaggtcgag
      901 cggctggcga cgatcgaccc gctgacggga agcgtgctgg acacgcacaa ggacatgtat
      961 atttatcctg caaaacactt cgttctgccc gaggagagaa tccagggggc cgtcgagaac
     1021 atcggcaacg agctggaagg acgattgaaa gatctcaagg agcaggggaa acttttggag
     1081 gcgcagcggc tggcggcgcg cacgcggtac gacatggaga tgctcatgga ggtgggctac
     1141 tgctcgggca tcgagaacta ctcgcgacat ctgtccaatc gcaagcccgg cgaaaccccg
     1201 accacgctgc tcgatttctt cccgccggat agcttgctga tcatggacga atcgcacgtg
     1261 acggtccccc aggtgcgggg aatgttcgca ggtgatttca gccgcaagag cacgctggtg
     1321 gatcatgggt tccggctcgc aagcgcgctt gacaaccggc cgttgcgatt tgatgagtgg
     1381 gagaaaaagt tcgaccggcg actgttcgtc tccgcgacgc ccagcgatta cgaagttgcg
     1441 atgtcagggg gcgaggttgt cgagcaggtc attcggccca ccggcctgat cgatccgatc
     1501 gttcgggtcg agccggcgcg aggacaggtc cctgccctgc tcaaggaagc caaggaacgg
     1561 gcggcgcgcg gcgagcgcgt tttgatcacc acgttgacca agagacttgc cgaagacctg
     1621 tcgcgctacc tgaaggaaca ggggttaagg tgcaagtggc tccactccga gctggacgcg
     1681 ttcgagcgag tcacgatcct ccgcgagctg cgcgaaggcg ctttcgatgc cttggttggc
     1741 gtcaacctgc tgcgcgaggg tctcgacctc cccgaagttt ccatggtttg tatcctcgac
     1801 gcggataaag aaggctttct gcggagcgag acctcgctga tccagacaat cggccgctcg
     1861 gcccggcatg tcaacgccga ggtcgtcctc tacgccgaca agatcacgcc gtcgatgcgg
     1921 cgggcaatcg acgagaccga gcgacgccgg gctctgcaat tggagtacaa taaagaacac
     1981 ggaatcacgc ccgagacaat cgtcaaggcg atccgccgtg ggattgaaga ggagatccag
     2041 gcgcggacca tcgtccgccg cgcggtcggt cgagacgaga cgacggatgc aaccgaggaa
     2101 tatctgagtg cgctcgaagc cgagatgctg gatgcggctg aaaaactcga attcgagcgg
     2161 gccgcggcac tgcgcgacaa gatcctcgag ctccgctcgg gcaatgacga cggcccgcgc
     2221 cgaccggccc catcgcctca ggctcaaagc gcgcgaacca aggcccgggc aaaagccagg
     2281 ggcgggcggc gaaagcaata agatttgatc atgatcggag gaagtcgacg ccaagtacac
     2341 ggcgtcatcc caaccgcaac ggtctgtcgc accggtccga tgcggcggaa aaaccagcct
     2401 tgtgctctca tatgacgatc aatttttctc gagtttccaa ggagtcattc atgcggcggt
     2461 ggttctgttt tctcgataag gacaagatgc cgactcaccg gaatcctgtc cggttggcgg
     2521 gtatacctgc gctcggggca ttgctcttat tcacggccgt cgtatcgatc ggttgcgact
     2581 cgaactcgtt cgtgccgccg cggccggaag agcttcgtgg aacggggtcg acaactttgg
     2641 caccgctgaa gaatgcttcg tcggacccag caggcctcga cttcgcgtcc gcgcgggctg
     2701 tcgagattgt cctggccccg cgcgacgggg acgaagctga agtctggaaa agcgcggctc
     2761 gcacccagtc aggtcacgaa aagatcaagg tgaaggtcac gatcgctgcc gcggatcaac
     2821 ccacgtcaaa gcaaatggag ttgatccgcg aggcgatggc ccgccatccc cgcgtgcttg
     2881 tcgtcgagcc ggccgaccct tcggacagtg ccctggctca ggcagtcgca gagacgcggg
     2941 cacaagggat accggtcgtg ctcttgggca ggccgctgac cggtgacaat aaaactcccg
     3001 cgaccgcaag cacctcgttg actgggccgc tcgtagtggt cgcgccgccc ccgttttctt
     3061 catccgcccg tgagctagtc gctgcggcga ttcgcaatgc caatgccgct gaactggatc
     3121 cgaatggtgg ggcgatcatc gtgattaaca cgctcggcga tcagttcata cccgaccgag
     3181 tcgccggaat ccgggacgcg ctcaagaccg cgggaatcac ctcggtcgag gaaatacgat
     3241 tcgagggcga tgtcacgaag ggcgaaaatc tcgtgaaaga cggcctcaag gctcatccga
     3301 aggctaccct gatcttcacg gttgactacc tgagcacctc ggcaatgcgc ggcctgctaa
     3361 acgcgacggc aaattctccg accttcgttg cgtgatgcta taccagcgat gccaacatgc
     3421 atgataccag catgcaaatg gccgtggcgg ccgcggtcga cttcacgccg gtgcgactgc
     3481 ttcgcaaggc gatcgctacg gccgccagcc tggcacaggg caaagtcatt tcgagcccag
//
LOCUS       DATAQL010001453         5007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2898212, whole genome shotgun sequence.
ACCESSION   DATAQL010001453 DATAQL010000000
VERSION     DATAQL010001453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2898212"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            604..903
                     /locus_tag="VHS97_25100"
     CDS             604..903
                     /locus_tag="VHS97_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX3451555.1"
                     /translation="MADKSSDSPVRIIGSRELHQNLPGILRELENDRVRYVLTVHGKP
                     RAVLIGAEPYLSMISDGGRSSEALIGLQLTALLGGSLDSVNLDDLEKALEQKVSG"
     gene            complement(935..1642)
                     /locus_tag="VHS97_25105"
     CDS             complement(935..1642)
                     /locus_tag="VHS97_25105"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX3451556.1"
                     /translation="MDQLWHVIEYVIELVRSVIELNPTTLNQYADSLGPWLYAVLFGI
                     IFAETGLVVTPFLPGDSLLFAVGAVAAHPASPIRLDLIALLLIIAAVVGDAVNYFLGY
                     KIGPRVFSREDSWLLNKKHLLQAHNFYEEYGGITIILARFIPIVRTFAPFVAGIGKMT
                     YWRFAIFNIMGGVAWILCFLLMGRWFGGQEFVQKNFKLVTLAIIVISVLPGVFEFIRT
                     RRSPNKPAIESSAQPID"
     gene            complement(1783..2217)
                     /locus_tag="VHS97_25110"
     CDS             complement(1783..2217)
                     /locus_tag="VHS97_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3451557.1"
                     /translation="MLASRVQSDEPPVINMTPMVDVILCLLVFFMAATRLYDWDESEF
                     AVNVPEVSEAAPATAAPDDLVLTVIKRGVVSMADTNYNLDQLTTLLREARGRYVNQGV
                     VIRGDASLAYQDLADVLSACDEAGIRNVRLPVRSRDERSKLP"
     gene            complement(2301..3056)
                     /locus_tag="VHS97_25115"
     CDS             complement(2301..3056)
                     /locus_tag="VHS97_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3451558.1"
                     /translation="MPPAPIDDSRRQPKPAVDRSSVLRLVLHANPMLWPLAICSIVTL
                     GYVMERFAALRRERVIPREFVERFLERLASGKLDRERALELCRAHESSAARIFSLVVN
                     AWGQPGATIRQVVSHDAAGEVVELKRNLRILSAMSTLGPLLGLLGTVVGIIQSFDALG
                     GRLGPARGEALAHGISLALVATAFGLAIAIVSVVFYYFFMNRVDVLVKELDDRTRQVI
                     DLVSGEFHRLPLADRRHGAIGSAESHRQESRAI"
     gene            complement(3291..4295)
                     /gene="ilvC"
                     /locus_tag="VHS97_25120"
     CDS             complement(3291..4295)
                     /gene="ilvC"
                     /locus_tag="VHS97_25120"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX3451559.1"
                     /translation="MPAKMYYDQDADLALLKGKTIAVIGYGSQGHAQAQNLRDSGCEV
                     VIGQRPGSPNYDAAVKDGFRPVSAAEAAEAADLINILLPDEVQGGVYTRDIEPHLRPG
                     DLLLCSHGFNIHFGQVVPPQGVDCALVAPKGPGHLVRSEFVKGGGVPCLIATTETCTP
                     GGKALALAYAKGIGGTRAGVLQTTFAEETETDLFGEQVVLCGGVSALVKMAFETLIEA
                     GYQPESAYFECLHELKLIVDLMYQGGLNYMRYSISNTAEYGDYTRGPRIVNESTRAEM
                     KKILAEIRSGQFAREWILENRANRPVFNATKRLEREHPVEQVGKRLRSLMPWIDAKTV
                     "
     gene            complement(4313..4951)
                     /gene="ilvN"
                     /locus_tag="VHS97_25125"
     CDS             complement(4313..4951)
                     /gene="ilvN"
                     /locus_tag="VHS97_25125"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX3451560.1"
                     /translation="MTTQFSSSAIPPAGGPAEKPDSAQKRHLLSALVQNQPGVLAHVS
                     GMFSSRGFNIESLAVGETENPHLSRITVVVMGDDRHLDQVRKQLEKIVTVVKVHDISR
                     EDYVERDLMLIKIGAPPARRPEINALVQIFRGRILDVSPDQLMIEISGQEKKIEAFIE
                     QVRPYGIHELARTGRIALVRGNRPLEAEAGTELAAANHAPEPEATVQGHSEL"
BASE COUNT         1015 a         1496 c         1493 g         1003 t
ORIGIN      
        1 cactcgaagc aacggctccc tggctcccga ccgactggcg tttgacgaca atccggaaac
       61 ggagcacttg cgtggcttcg atcgacctgt cttgcggcta gggcctggca cgctcgctcg
      121 actcactgct gaagctacgc cgcgcgacgg aacgggttga ttgtccgtcg tctgtattgc
      181 ctgatccgtt gtccgctgcc ggtgacacgc tcgaatcgat cgctacgcca aacgagacga
      241 tccgttggcg cacccgctta aatcgccggc ggtgaaccaa cgaccaacga aagcagaccc
      301 ttgcatttgg gcaatccgtc tgtaaaatga catgaccgca aggcggcaag cacgtcagaa
      361 ttaccgaact ctcgggatgg acggcttttg ggaccagggt cggagccagt tgtctctttc
      421 tcggcccccg ttcgtggcga agcggatcaa gggatagcgt ggtcccgcca tagggcttga
      481 gtattatagg tccgttcgac tgctggtcga tcgcgctcta tttgagctgt tttcgtcgtc
      541 cgtcgaccaa gcctaggtcc tcgtgttccg tactgccgct ggtccaaggg aggcacatct
      601 ttcatggcgg acaagtcttc ggactctccc gttcggatta tcgggagtcg ggagttgcac
      661 cagaatctgc cgggaatctt gcgagagctt gaaaacgatc gtgtgcgtta tgtcctcacc
      721 gtccacggca agccgcgcgc tgtgctgatt ggagccgaac cctacctgag catgatcagc
      781 gacggcggtc ggtcaagtga agcgttgatc gggcttcagc tcactgccct gttgggcgga
      841 agtctcgatt ccgtaaacct ggatgacctc gaaaaggctc tcgaacaaaa ggtcagcggt
      901 taattcatca tcaaagcctg ccgcgcggcc gctctcaatc aatcggctgc gcgctgctct
      961 cgatcgctgg cttattgggg ctgcgccgcg ttcggatgaa ctcgaatact ccggggagca
     1021 ccgagatcac gatgatcgcg agggtcacca acttgaaatt cttctgaacg aactcttggc
     1081 ccccaaacca ccggcccatc agcaagaagc agagtatcca ggcaactccc cccatgatat
     1141 tgaaaatcgc aaatcgccag taggtcatct tgccaatgcc ggccacgaac ggtgcaaacg
     1201 tccgcacgat ggggataaaa cgcgccagta tgatggtgat gccgccatac tcctcataga
     1261 agttgtgggc ctggagcaaa tgctttttgt tgagcagcca ggaatcctcg cgcgagaaca
     1321 cgcgggggcc aatcttgtag ccaagaaagt agttgactgc atcgcccacg accgccgcga
     1381 tgatcaagag cagggcgatt aggtcgagcc ggatcggcga cgcaggatgg gcggcgacgg
     1441 cgccgaccgc gaagagcagc gagtcgccgg gcaagaaagg cgtcaccacc agccccgttt
     1501 ccgcgaagat gatcccgaac aagaccgcgt aaagccaggg gcccagtgag tcggcgtact
     1561 gatttagagt cgtgggattg agttcaatca cggaacgtac aagctcgatc acatactcga
     1621 tcacgtgcca aagttgatcc atcctctgct ccgttgcgat gtttttatgt tggagggcaa
     1681 tcgggtcgct ttaagtcgtt cgattcatct tacactaaca gatttgctct tcgaggatgg
     1741 tcgggtcgcg gcaggcccgg tcttggtcgg caaccgccgc ggtcacggca atttcgatcg
     1801 ttcgtctcga gacctgacgg gaagcctgac attgcgaatt cccgcttcgt cgcacgccga
     1861 caggacgtcg gccaggtcct gataggcgag actcgcgtct ccgcggatca cgaccccctg
     1921 gttgacgtag cgcccacgcg cctcgcgcag caatgtcgtg agctgatcga ggttataatt
     1981 cgtgtccgcc atcgagacga cgccgcgctt gatcacggtc agtacgagat catcgggggc
     2041 ggccgtggcg ggggcggcct cagaaacctc gggaacattc acggcaaact cgctctcgtc
     2101 ccaatcatac aaacgcgtgg cggccatgaa gaacaccagg aggcaaagaa taacgtcgac
     2161 catgggcgtc atgttaatga cgggcggctc gtcggattga actcggctgg cgagcatctt
     2221 ggcaaacccc gcgccagttt tgggcgcttc gacctttggg ttctatcgtc gacatttaca
     2281 aggcttctcg tgcgacggcg tcagatcgcg cgcgactcct gacggtgaga ctcggccgat
     2341 ccaatcgcgc cgtgacgccg atccgccagc ggcagacgat gaaactcgcc agagacgaga
     2401 tcgatgacct gacgcgtgcg gtcgtccagc tctttcacca gtacatcaac tcggttcatg
     2461 aagaagtagt agaacacgac cgagacgatg gcgatcgcca ggccaaaggc agtggcgacc
     2521 agggccaggc tgatgccgtg ggccagggcc tctccccggg ccggccccag ccggccgccg
     2581 agtgcgtcga acgactgaat gatccccaca acggttccca gtaagcccag gagaggtccc
     2641 aaggtcgaca tggcactcaa tattcgcaag ttacgtttga gttcgacgac ttcaccggcc
     2701 gcgtcgtgac tcacgacttg gcggatcgtc gcgccaggct gaccccaggc gttcaccacc
     2761 aacgaaaaga tccgtgcagc agaactttca tgggcccggc aaagctcgag cgctcgctcg
     2821 cgatcgagct tcccactcgc caaacgttcg agaaatcgtt cgacaaactc gcgagggatg
     2881 acccgttcgc gacgcaaggc ggcgaacctt tccatgacat aacccagtgt cacaatcgaa
     2941 cagatcgcca gcggccagag catgggattg gcgtgcaaca caagccgcaa gacgctggat
     3001 cgatcaaccg ctggcttggg ctgccgcctg gagtcatcga tcggcgcggg gggcacgctg
     3061 ggctcggcgg aaagagctga cgcaccggcg gatcctctct tgtttgcatc ctcggttggc
     3121 gaggcggatt gagctcgcgc cggctgtcga gacaagagac caagaagaca aagacacagg
     3181 ccgagaacat gagccaggac tcgcggtttg ccgaccatcg tttcggatcc tccaggttag
     3241 caaccgccgc atcgtatcgc acagcccggc attttcctag cgcggcaggg tcaaacggtc
     3301 ttggcgtcga tccatggcat caggctgcgc agacgtttgc cgacctgttc caccgggtgc
     3361 tcgcgctcca gccgcttggt cgcattgaac acgggtcgat tggctcggtt ttccaggatc
     3421 cattcgcggg caaactggcc cgaacgaatc tcggccaaaa tcttcttcat ctcggcacgc
     3481 gtcgattcgt tgacgatgcg cggacctcgg gtgtaatcac cgtattcggc ggtgttggag
     3541 atgctatacc tcatgtagtt gagcccccct tgatacatga ggtcgacgat gagcttgagc
     3601 tcatgcaggc attcgaaata cgcgctttcg ggttgatagc cggcctcgat cagagtttca
     3661 aacgccattt tcacgagggc gctcacgccg ccgcaaagga cgacctgttc gccgaagagg
     3721 tccgtttcgg tttcctcggc aaacgtggtc tgcaacacgc ccgctcgggt gccaccgatc
     3781 cccttggcat acgcgagtgc cagggccttg ccgcccggtg tgcaggtttc cgtcgtggcg
     3841 atcaagcagg gaacgcctcc acctttcacg aattcactcc gaaccaggtg gcccggaccc
     3901 ttgggggcga ccaaggcaca gtcgacacct tgggggggaa cgacctggcc gaaatgaatg
     3961 ttgaacccat gcgagcaaag caagagatcg cccggccgca gatgaggctc gatatcgcgg
     4021 gtgtagacgc ccccctgaac ctcgtcgggc aaaaggatat tgataaggtc ggcggcttcg
     4081 gcggcttcgg cggctgatac tggacgaaac ccgtctttga ccgcggcatc gtaattgggc
     4141 gagcccgggc gctggccgat cacgacttcg caacccgagt cgcgcagatt ttgagcctgg
     4201 gcgtgaccct ggctgccata accgatcacc gcgatcgtct tgcccttcaa aagcgcgagg
     4261 tcggcatcct ggtcgtaata catcttggca ggcatcggcg tttcctcttt cttcagagct
     4321 ctgaatgccc ttgcacagtc gcctcgggct caggggcgtg atttgcagca gccagctcgg
     4381 tgccggcctc tgcctcgagc ggacgattcc ctcgcaccag ggcgatccgg ccggttcgcg
     4441 ccagctcgtg aatgccgtac gggcggacct gttcgatgaa tgcctcgatc ttcttttcct
     4501 gtccggaaat ctcgatcatc agttggtcag ggctcacgtc gagaattctg ccacgaaaga
     4561 tttgaaccaa ggcgttgatc tcaggccggc gcgcgggggg cgcgccgatc ttgatcagca
     4621 tcaaatcgcg ttcaacatag tcttctcgtg aaatgtcgtg gaccttcacg accgttacga
     4681 tcttctcgag ctgcttgcgc acttgatcga ggtgccggtc gtctcccatc acgaccaccg
     4741 tgattcgcga gagatggggg ttttcggtct cgccgaccgc caggctctcg atgttgaatc
     4801 cgcgcgagga aaacatgcct gagacgtgcg ccagcactcc aggctggttt tgcaccaggg
     4861 ccgagagcag atggcgtttt tgggcggaat cgggcttttc ggccggtccg cccgcgggcg
     4921 gaatggcact cgagctgaac tgggtcgtca tggtggtcac ctgcggttgc gagctactcg
     4981 tcgtgagtcc gcctcagcgc ggccgta
//
LOCUS       DATAQL010001454         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3662579, whole genome shotgun sequence.
ACCESSION   DATAQL010001454 DATAQL010000000
VERSION     DATAQL010001454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3662579"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1989)
                     /locus_tag="VHS97_25130"
     CDS             complement(184..1989)
                     /locus_tag="VHS97_25130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX3451561.1"
                     /translation="MLHLIAMLLAAAPGLASPADGAVSPPAVGAAVAEFRLNDVHRRP
                     RSIGGFKDKKAFVVAFVDTECPVSNLYVPALIDLHRKYTNNGVQFLAINSSGQDSFVS
                     VSAHAQERDIPFPVLKDFDQNVADAFGATRTPEAFVLDANRVIRYHGRIDDQYGVGVR
                     RDGPTRRDLAEAIDDVLAGRSVALPDTEVSGCPLERRRNSREGVDLTYARHVAPILQK
                     RCQECHRPGEIGPFSLLSFEDAEKRTGRIREAVLEERMPPWHADPRHGKFANDRRLTQ
                     EEHDTLLAWIDQGAKKGDDKDMPPAVTFTQGWKIGEPDKVFSMTEEFKVPATGVLDYQ
                     RFVVDPGFKTDVWVRAAECRPGNRKVVHHILVYILAPGRREPYEADGTASTLVGWAPG
                     DMPATYARDTARLIAAGSKLVFEVHYTPDGTEQIDRSSVGIRFAQGKPPQSVETNILA
                     NMLFIIPPKASNYKGQMTYTFPADALVLSFMPHMHLRGVSAEYVLTRPDGTIETLLAV
                     PDYDFGWQSVYRFAEPLKVPKGSKLMWTGHWDNSADNPRNPDSTKAVHWGLQTWDEMQ
                     NGWMEVVWKTPKPKSHPTGLAPRQSQGGAEPVLQR"
     gene            2200..3081
                     /locus_tag="VHS97_25135"
     CDS             2200..3081
                     /locus_tag="VHS97_25135"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX3451562.1"
                     /translation="MPELPDITLYIEALDKRILGERLESVRIQSPFLLRTFEPPLKEA
                     EGKTVRTLRRIGKRIALGLDNDLWLVLHLMIAGRLHWKPPGATLLGKSSLASLSFSSG
                     TLQLTEAGTKHRASLYFLRGEDALRDQDPGGLEPLTATFEQFVAALRRENHTLKRSLT
                     DPRLFSGIGNAYSDEILHQARLSPLALTHKLTEDEAKRLYTATRGSLVEWVDRLRAET
                     GDGFPEKVTAFRPGMAVHGRFGELCPVCAAPVQRIRYADNETNYCPRCQTGGRILADR
                     SLSRLLKDDWPRSLDEL"
     gene            complement(3094..>3694)
                     /locus_tag="VHS97_25140"
     CDS             complement(3094..>3694)
                     /locus_tag="VHS97_25140"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX3451563.1"
                     /translation="WDYDNDGWLDFYLGTGESSLATLVPHRMFKNVSGERFASITAFS
                     GTGHLQKGHGVACGDWDRDGDVDVFIEAGGAVNGDKYDSILFQNPGQGNHWLTLKLIG
                     KKTNQAAIGARIKVVTAGENPLTIYRHISSGSSFGANTLQQTIGMARAQRVAILEIHW
                     PASGTTQVFRDIAADQAIEVTEFAESIRPLDWRPVPQPQ"
BASE COUNT          695 a         1129 c         1107 g          763 t
ORIGIN      
        1 atgaagcaaa gccttaccgg ccaatgagcc gcccatggct cgaatcccag tcgtgttacc
       61 acaggcgccg gcactatgtt cattacgtga acgcagagcg gcattgaaac tcgccgtgcc
      121 tgcgttgacc gcaaccatcc ctgtgctggc ggccgctccg gccggttcag acgaccccct
      181 tgatcatcgt tgcaagacgg gttcggcacc accttgggat tgccgcggtg ctagaccggt
      241 tgggtgcgac ttcggtttcg gcgtcttcca caccacctcc atccagccgt tctgcatctc
      301 gtcccaggtt tgcaagcccc aatgaaccgc cttggttgag tctgggttgc gcgggttgtc
      361 tgcggagttg tcccagtgac cggtccacat gagcttgctt cctttgggga ccttcagcgg
      421 ctcggcgaaa cggtaaacgc tctgccagcc gaaatcgtag tccggcaccg ccagcaatgt
      481 ctcgatcgtg ccatcgggcc gcgtcaacac gtactcggcg ctcacacctc tcaagtgcat
      541 gtgcggcatg aacgatagaa cgagagcatc ggcggggaac gtgtacgtca tctgcccttt
      601 gtaattcgag gccttgggcg ggatgatgaa cagcatgttc gccagaatgt tggtctcgac
      661 ggattgagga ggcttgccct gtgcgaaccg gattccgacc gacgagcggt cgatctgctc
      721 ggttccatcg ggggtatagt ggacctcaaa aaccagcttg gagccggcgg caatgagccg
      781 ggccgtgtcg cgcgcatagg tcgctggcat gtcgccgggc gcccagccca ccagggtcga
      841 cgccgtgccg tccgcctcgt agggttcgcg gcgaccgggt gctaggatgt aaacaaggat
      901 gtgatggacg accttgcggt tgcctggacg gcactcggcc gcccgcaccc aaacgtcggt
      961 tttgaaaccg ggatcgacaa cgaatcgttg atagtccagg acaccagtgg ccggcacctt
     1021 gaactcttcg gtcatcgaga aaactttgtc cggctcacca atcttccagc cctgtgtaaa
     1081 cgtcacggcc ggaggcatgt ccttgtcgtc ccctttcttg gcgccttggt cgatccaggc
     1141 caaaagggtg tcgtgttctt cttgtgtcag gcgtcggtcg ttggcgaact taccgtgacg
     1201 tgggtcggcg tgccaggggg gcatccgctc ttctagaacc gcttcgcgaa tgcggccggt
     1261 ccgtttctcg gcgtcttcaa atgagagcag tgagaacggt ccgatctcgc ccggccgatg
     1321 acattcctga cagcgttttt gcaggatcgg cgccacgtgc cgcgcatagg tcaaatccac
     1381 gccctcgcgc gaattgcgac ggcgctcgag tgggcagccc gacacttcgg tgtcaggcag
     1441 tgcgaccgaa cgccctgcca gcacgtcgtc gatcgcctcc gccagatcgc gccgggtcgg
     1501 accgtcgcgg cggacgccga cgccgtactg atcgtcgatc cgaccgtgat agcggatcac
     1561 gcgattcgca tccagcacga acgcctcggg cgtgcgggtc gcaccaaatg cgtcggccac
     1621 attctggtcg aagtctttga gaaccggaaa tgggatatcc cgctcctgtg catgtgcaga
     1681 cacgctgaca aacgagtcct ggccgcttga attgattgcc aggaactgaa cgccattgtt
     1741 ggtgtacttg cggtgcaggt cgatcaaggc cggcacgtag aggtttgata caggacattc
     1801 ggtgtcgaca aatgcgacga caaaggcttt cttgtccttg aaaccgccga tcgagcgggg
     1861 gcggcgatgg acgtcgttca agcgaaactc agcaacggcc gcacccaccg cagggggaga
     1921 gaccgcccca tccgccgggc tcgccaatcc gggcgcggca gccagcagca ttgcgatcaa
     1981 gtgcagcacg attcgttcct ccgggaaatg gtgagcgtgg ttacgccgac gttcgcgccc
     2041 gcgcgagggt agcacaccgc gtcttggcga ttataccatg gaaccgcggc tttctcgaag
     2101 ccattggtgt cgcctttgac gcggaccacg gggtgtaatt gcagctagga gtcggtgcga
     2161 tcgtgttttc tggttcttga tccgaggact ctcaacctca tgcccgaact tccggacatt
     2221 accttgtaca tcgaggcgct cgacaaacgc attctgggcg agcggttgga gtcggtccgg
     2281 atccagagcc catttctgct ccgaacattt gaaccacctt tgaaagaagc cgaaggcaag
     2341 acagtccgca cgctgcgacg aatcggcaag cgaatcgcgc tgggactcga taatgatctc
     2401 tggctggtgc tccacctgat gatcgccgga cggctgcatt ggaagccgcc tggagccaca
     2461 ctcctgggaa aatcctcctt ggcgtccttg tcgttttcca gtgggacttt gcagctcacg
     2521 gaggccggca cgaaacaccg cgcttcgctc tacttcctgc gaggggagga tgccctgcgc
     2581 gatcaagacc caggcggcct cgaaccgctc actgcgactt tcgagcaatt tgttgcggcc
     2641 ttgcgccggg aaaaccacac actcaagcga tctttgaccg atccgcggct cttcagcggc
     2701 atcggcaatg cctattccga tgagatcctt catcaggctc ggctctcacc gctggcactt
     2761 acgcacaagc tcacagaaga cgaggcgaag aggctttaca cggccacgcg cggatcgctc
     2821 gttgaatggg ttgaccgtct ccgtgccgag accggcgacg gctttcccga aaaggtcacc
     2881 gcctttcgac cgggcatggc cgtgcatggt cgattcggag agttgtgtcc cgtctgcgcg
     2941 gcccctgtgc aacggatacg ctacgcagac aacgaaacaa actattgccc acgttgccag
     3001 accggcgggc gcatcctcgc cgaccgatca ttgtctcggc tactcaagga tgattggccg
     3061 cgatcgctgg atgagctttg aaaatccgca ctctcattgc ggctgtggca cgggccgcca
     3121 gtcgagcgga cggatcgact cggcgaactc ggtcacttcg atcgcctggt cggcggcgat
     3181 atcgcggaag acctgggtcg ttccgctggc cggccagtgg atctccagaa tggccactcg
     3241 ctgcgcgcgc gccataccga tcgtttgttg caacgtattg gctccgaaac tactgccgga
     3301 tgaaatgtgc cggtagatcg tcagaggatt ttcaccggcc gtgaccacct tgatccgtgc
     3361 gccgatggct gcctgattgg tcttcttgcc gattagcttc aacgtcagcc aatgatttcc
     3421 ctgcccgggg ttctggaaga gaatactatc atatttgtca ccgttgacgg ctcccccggc
     3481 ttcgataaat acgtcgacgt cgccgtctct gtcccagtcg ccgcaagcca cgccatggcc
     3541 cttttgcaag tggccggtgc cggagaacgc cgtgatcgag gcgaaccgct cgccgctgac
     3601 gtttttgaac atgcgatggg gtacgagcgt ggccagactg gattctccgg tgcccaagta
     3661 gaagtcaagc cagccgtcgt tgtcgtagtc ccac
//
LOCUS       DATAQL010001455         6119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3052504, whole genome shotgun sequence.
ACCESSION   DATAQL010001455 DATAQL010000000
VERSION     DATAQL010001455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3052504"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHS97_25145"
     CDS             complement(<1..224)
                     /locus_tag="VHS97_25145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451564.1"
                     /translation="MAGITVVAPQAPAHCSLRESRAALKLAVRALTATIPVLAAVPAG
                     ADDPPHYRFKTGSARPWDCRGARPVGCDFG"
     gene            complement(232..924)
                     /locus_tag="VHS97_25150"
     CDS             complement(232..924)
                     /locus_tag="VHS97_25150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3451565.1"
                     /translation="MGFRTPNIDRIAREGMIFTDYYAEQRCTAGRSSFITGQCTFRTG
                     LSKVGVPGATVGLKKEDPTIAELLKPHGYATGQFGKNHLGDRNEYLPTAHGFDEFFGN
                     LYHLNAEEEPEQRTYPRDPTFKERFGPRGVLRCKATEQDDQTQQPRWGRVGKQVIEDT
                     CPLTKKRMETIDDETSDAAVDFSQPYLVFAAQVVTSKFLDTFKEFPPRQRAASFSIDQ
                     AVEKMKQSLTSQ"
     gene            complement(930..1253)
                     /locus_tag="VHS97_25155"
     CDS             complement(930..1253)
                     /locus_tag="VHS97_25155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451566.1"
                     /translation="MIPKYASIRALAVVASGALLGGTAATADLPRSAVPSKQEAAGGA
                     ILVGKAADAAKTCCSSDLARGSLLALAEHNQSVAANAAQAGKNPNFRSYSARISARRM
                     SAPIR"
     gene            1642..2067
                     /locus_tag="VHS97_25160"
     CDS             1642..2067
                     /locus_tag="VHS97_25160"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3451567.1"
                     /translation="MKTNGSAVWQGGLKDGKGAISTRSGALKDYPYGFNSRFEGKPGT
                     NPEELIGAAHAGCFTMALSLILGEAGLTAEHMETTAEVTLDKVDDGYAITAVHLTLKA
                     KIPGADQAKFDQLTAMAKAGCPVSKLLNARITLDATLAT"
     gene            complement(2090..2710)
                     /locus_tag="VHS97_25165"
     CDS             complement(2090..2710)
                     /locus_tag="VHS97_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3451568.1"
                     /translation="MRYAAGQKQRTRAKIVRAAGKVFRREGYHAAGVDKVMEEAGLTA
                     GGFYAHFVSKEALLAATLEPTAAEVGGRRDKELESLAGRAWVEAFIERYLAPQHMRNT
                     EGGCPLPALASEVARAGSPVKASFESVVRGFAARLVDCAGGELTEERALAIVALCVGG
                     LGVARSVKDDALSERILASCRELAKASLAADPQSEAKAKPRLRKAK"
     gene            complement(2746..3660)
                     /locus_tag="VHS97_25170"
     CDS             complement(2746..3660)
                     /locus_tag="VHS97_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX3451569.1"
                     /translation="MTADAPILVTGAAGRVGGVGRDVVEILRRRGLPVRAMVYREDER
                     AQALRAMGAQVVVGDLTRADDVARALDGCRRMYFGMSVSATYLEATIIAAAVARQRGD
                     LEVFVNISQLTVSQMSLTEMTDSPQHRQHWLGEQALDWSGLPVVHIRATVFLQHPFFS
                     AWAAESIAADNTIRLPFGKGRTSPVDAADVAEVVAAVLSSPELHIGKVYELTGPRSQD
                     MNGVAAECSDALGRTITYVDIPFQLWLEQELGARHLPEHVFEHLRTMARLHAENRYDR
                     LTQDVLAVTGRPATSVRDYVAKHPELFG"
     gene            3993..4838
                     /locus_tag="VHS97_25175"
     CDS             3993..4838
                     /locus_tag="VHS97_25175"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3451570.1"
                     /translation="MERRAFGSTKHEVPVIGQGTWYIDHDDRSTAIAALRRGITLGMT
                     HVDTAEMYGAGKAEEMVAEAIAGKRDEVYLVSKVLPENASRRGTIRACERSLDRLQTD
                     RLDCYLLHWRGRYPLEETIAGFEQLRREGKIRSWGVSNFDVPDLEEVSALVGSGNLAC
                     NQVLYHLQERAIEHAVIPWCASHGVAVVAYSPFGRGNFPGPRTSARQVLEEIADKHHA
                     TSHQVALRFLVRHPSLFTIPKATSPDHAAENASAGDLELSEAELALIDRTFPLGPRPS
                     DLPTL"
     gene            4862..5008
                     /locus_tag="VHS97_25180"
     CDS             4862..5008
                     /locus_tag="VHS97_25180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451571.1"
                     /translation="MLSRLLEAHEITLNDAHGAAAKVAEHGDNGTNDLILSDVIRSGE
                     LQAW"
     gene            5050..5334
                     /locus_tag="VHS97_25185"
     CDS             5050..5334
                     /locus_tag="VHS97_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX3451572.1"
                     /translation="MICEHVVNLESVPPGTLGCEECLKTGTPWVHLRLCLTCGHVGCC
                     DSSPGRHATKHFHRSGHPVIASFEPGERWAWCYIDEAELPVPEPVTPYLR"
     gene            complement(5583..>6119)
                     /locus_tag="VHS97_25190"
     CDS             complement(5583..>6119)
                     /locus_tag="VHS97_25190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451573.1"
                     /translation="VIQEFTANPQGLIYSSPAYFDGKVYIQGVGDVIKAFALKLDPAT
                     NTMMLDETPVTQGNIVSGFPGTVESISADGTSNGIVWSAQVDGFASDGPAILRAYEAN
                     NLATPLYASNQAGPRDTAGGGVKFTTPTIANGHVYLGTQFEVDAYGLLPTAGSASNSV
                     RTAARTVRIPSRVPSPGA"
BASE COUNT         1208 a         1887 c         1854 g         1170 t
ORIGIN      
        1 aaaccgaagt cgcacccaac cggtcttgca ccgcggcaat cccaaggccg tgccgatccc
       61 gtcttgaaac gataatgagg cgggtcgtcg gcaccggccg gaactgccgc gagcacaggg
      121 atggtcgcgg tcaacgcacg cacggcgagt ttcaatgcgg ctctgctttc gcgtaacgaa
      181 caatgcgccg gcgcctgtgg tgcgacgact gttattccag ccatgggcgg ctcattggct
      241 ggtaaggctt tgcttcatct tttccacagc ctgatcgatg ctgaaactgg cggcccgctg
      301 ccggggcggg aactctttga acgtatcgag gaacttggac gtcacaacct gggctgcaaa
      361 aaccaagtac ggctgcgaga agtcaacggc cgcgtccgac gtctcatcgt cgatcgtctc
      421 catccgcttt ttcgtcagcg ggcaagtatc ctcgataacc tgcttaccga ctcgtcccca
      481 ccgcggttgc tgggtttggt catcctgctc ggtcgcctta cagcgcagta cgccgcgggg
      541 accgaatctc tccttgaaag tcgggtcacg cgggtaagtc cgctgctcgg gctcttcctc
      601 tgcgttcaag tggtagagat tgccgaagaa ctcgtcgaag ccgtgggcgg taggaaggta
      661 ctcattgcgg tcgccgaggt gattcttgcc gaactgcccg gtcgcgtagc cgtgcggctt
      721 caacagttcg gcaatggtgg gatcttcctt cttgaggccc acggtcgccc cagggacgcc
      781 gactttgctc aggcctgtgc gaaaggtgca ctgtcccgta atgaacgacg accggccagc
      841 agtgcatctc tgctcggcat agtagtctgt gaagatcatc ccttcgcgag cgattcggtc
      901 gatgttcgga gtacggaagc ccatcagccc tatcgaatag gcgctgacat tcgtctggcc
      961 gatatccttg ccgaatatga ccggaagttc ggattctttc cggcctgggc cgcattcgcg
     1021 gcgacggact gattgtgttc cgccaatgcc agtagtgacc cgcgggcgag gtcactcgag
     1081 caacacgtct ttgccgcatc cgcggctttg ccgaccaaaa tcgcgccgcc cgcggcctct
     1141 tgtttggatg gaaccgccga tcgcggcagg tccgcggtcg ctgccgtgcc acccaagagc
     1201 gcaccggacg ccaccacggc caaggcccga atactcgcgt atttcgggat catgttcctc
     1261 tcctgctcta ccaaagcttt tcgacatgtg agtgcggacg acttgcgagc catgtgagcg
     1321 ttcgtcggcg ggtacgctgc gtcgagcaaa atgttggcaa ggccgcatgg ccgtaagacg
     1381 acctgcaatg acgcccgtct cgaaactcta actggcgcct acaagcgagt ccagcatttc
     1441 gtaagaaagg atgagcaata acggcacttg cccgccagac taaatccggc agtacgacaa
     1501 tctcgaaacg agacggccat gaaggtccgc tcgcgttatg tctgcggcgc atcaccacga
     1561 cgctcgacgc gcgaggtttg tgccgttaaa attgcttatc cgttggatcg atttcgacga
     1621 ccaaaccagg gagagctcac gatgaaaacc aatggatcgg ccgtgtggca gggcgggctc
     1681 aaggatggca agggagcaat ctcgactcgg agcggtgcgc tcaaggacta tccctacggc
     1741 ttcaacagcc gcttcgaggg caagcccggc acgaaccccg aagagttgat aggggcggcc
     1801 cacgccggtt gcttcacgat ggcactgtca ttgatcttgg gagaggccgg cttgacggcc
     1861 gagcacatgg agacgaccgc cgaggtcacg ctcgacaagg tcgacgacgg ctacgccatc
     1921 acggcagtcc acctgaccct gaaagccaag attccggggg cagaccaggc gaaatttgac
     1981 cagctcacgg ccatggccaa agccggttgt cccgtgtcga aactcttgaa tgccaggatc
     2041 accttggacg cgacgctggc gacctgacgc atcgatcgga gaacatttgc tacttggcct
     2101 tgcgtagtcg aggcttggcc ttcgcctccg attgaggatc ggcggccaga ctcgcctttg
     2161 ccagttcgcg acaagacgcg aggatccgct cgctcaaggc gtcgtccttg accgatcgcg
     2221 ctacccccag cccaccgacg cacagcgcga cgatcgcgag tgccctttcc tccgtgagtt
     2281 cgccccccgc gcagtccacg agacgcgcgg cgaagccgcg aacgacggac tcaaagctcg
     2341 ccttcacggg acttcccgcg cgagcgacct cggaggccag tgccggcagc gggcagcccc
     2401 cttcggtgtt cctcatgtgc tgcggggcca ggtagcgctc gataaaggct tcgacccagg
     2461 cgcggcctgc caagctctca agctctttgt cgcgccggcc gcccacttcg gccgcggtgg
     2521 gctcgagcgt cgcggccaaa agcgcctcct tcgacacgaa atgagcgtag aagccgccgg
     2581 ccgtgagccc tgcctcttcc atgaccttgt ctactcccgc cgcgtgatac ccctcacggc
     2641 ggaaaacctt accggccgcc cggacgatct ttgcccgggt cctttgcttt tgaccggccg
     2701 catagcgcat gggatgcctc caccgaacga atccttgtta gaatttcatc cgaacaattc
     2761 cgggtgcttg gcgacgtaat cgcgcacgct cgtcgcgggc cttcctgtga ccgccaaaac
     2821 gtcttgggtg aggcgatcgt agcggttttc ggcgtgcagg cgagccattg tccgcaggtg
     2881 ctcgaaaacg tgctctggaa gatggcgggc gccaagctcc tgttcgagcc acagttggaa
     2941 tgggatgtcg acatacgtga tcgtccggcc tagggcgtcg gaacattcgg ctgcgacgcc
     3001 gttcatgtcc tgcgatcgcg ggcctgtcaa ttcgtagact ttgccgatgt gaagctccgg
     3061 actggacaaa acggccgcaa ccacttccgc cacgtccgcg gcgtcgaccg gcgaggtccg
     3121 gcctttgcca aacggaagcc ggatggtgtt atccgcggcg atcgactcag cggcccaggc
     3181 cgagaagaac gggtgctgaa ggaagaccgt cgcgcggatg tgcacgacgg gcaatcccga
     3241 ccagtcgagc gcctgctcac ccagccagtg ctgacggtgc tggggagagt cggtcatctc
     3301 ggtcagactc atctgcgaaa cggtcagctg ggaaatatta acgaatacct cgagatcacc
     3361 acgctggcgc gcaacggcgg ccgcaatgat agtcgcctca aggtaggtcg ccgacacgct
     3421 catcccgaaa tacatccgtc gacagccgtc gagcgcacgg gcgacatcgt cggcgcgggt
     3481 gagatcgccg accacgacct gggcgcccat tgcccgcagc gcctgggcgc gttcgtcctc
     3541 acgataaacc attgcacgca ctggcagacc gcgtcggcgg agaatctcca cgacgtcgcg
     3601 tcctacacca ccaacgcggc ccgctgcgcc cgtgacgaga atcggggcgt cagccgtcat
     3661 cgatgcactt ccaatgccaa acagacccgc tccgggtcct cacgcgagcg ctgccacgcc
     3721 ttttacactg cgatcggaaa agatccaccc tgacgcattc gtgtcaacaa acagacagat
     3781 ccacagaccg acgaacaata atatcacgtt cgtaatataa aggcaacggt catgttacta
     3841 tcagcgttta aagtggcccg aacggtcgaa cccttttctg cccccgaatt gcgcggcaat
     3901 ccccgagact cccgcaagcc tgtggaaccg agtggcgagg attgtcatca tggcgtaatt
     3961 ctgatattcg tcagtggacc aagggggaag acatggaacg gcgggcgttc ggttccacga
     4021 aacacgaggt tccagtaatc ggtcaaggaa cctggtacat cgaccacgat gaccgcagca
     4081 ccgccatcgc cgccctccgc cggggtatca ccttgggcat gacccatgtc gacacggcgg
     4141 agatgtacgg cgcgggcaaa gccgaagaga tggttgccga ggctatcgca gggaaacgcg
     4201 atgaggtgta tttggtctcg aaagtcctgc cggaaaatgc atcgcgtcgc gggacaatca
     4261 gggcgtgtga gcggtccctg gatcgtcttc aaacagaccg gctcgattgc tatctgcttc
     4321 attggcgtgg ccgataccca ctggaggaga cgatcgcggg tttcgagcaa ttgagacgcg
     4381 aaggaaagat ccgctcctgg ggagtgagta attttgatgt gccggatctg gaggaggtaa
     4441 gcgcgctcgt gggcagcggc aacctcgctt gcaatcaggt gctgtatcat ctccaggaac
     4501 gagccatcga gcacgcggtc attccctggt gtgcgagcca cggggttgcc gttgtcgcct
     4561 acagcccgtt cggccgcgga aactttcccg ggccacgcac aagcgcgaga caagtgctgg
     4621 aagaaatcgc cgacaagcat catgcgacgt cccatcaagt cgctctccgg tttctggtac
     4681 gccatccgtc gcttttcacg atccccaagg ccaccagccc cgatcatgcc gccgagaacg
     4741 cgagtgcggg agatcttgaa ctttccgagg ctgaactagc actgatcgat cggacctttc
     4801 ctctcggtcc acgccccagc gacttgccga ctctatagtc ttctgcgaag aagtgcctgc
     4861 aatgctttcg cgactcctgg aggcacacga gatcaccctc aatgacgccc acggcgcagc
     4921 cgcgaaggtt gctgaacacg gcgacaacgg tactaacgat ttgatcttat cggacgtcat
     4981 ccgctccgga gagctgcaag cttggtgaat cccccaatcc aaggaatctc tgcgtcggga
     5041 gtattactaa tgatctgtga acatgtggtg aatctcgagt cagttccacc cggaactctg
     5101 ggctgcgaag agtgtctgaa gacggggacg ccctgggttc atctgcgtct ctgcctgacg
     5161 tgcggccacg tgggctgttg tgacagttcg cctggccggc acgccaccaa gcacttccat
     5221 cgcagcggtc acccggtgat agcgtccttc gagccaggag agcgctgggc gtggtgttac
     5281 atcgacgagg cggaactccc agtgccggaa cccgtcacgc catacttgcg gtgacgaaat
     5341 agtgcacatt cgaatcgtgc aaccggacgt tgatgccgta ttcacatata cgacaatgcg
     5401 ttataacatg tcgctggatt aatcggcgta tcatgatttg tgggtataaa aggagcgtcc
     5461 ttgcaagggg aacctgcgtc ccggcgcgat tggcggcggg tgcggtggtg gtgatgccga
     5521 ccaccaccgc accgatcgtg gggaggggcc ccgcggctga aaatctcaag ggattctcga
     5581 tcctacgcgc ccggcgacgg caccctgctt gggattcgaa cggtgcgggc cgcggtcctg
     5641 accgagttgg acgcgctacc cgccgtcgga aggagtccgt aggcgtcaac ctcaaattgc
     5701 gtgccgaggt agacgtgccc gttggcgatc gtcggcgtgg tgaacttgac cccgccgccg
     5761 gcggtgtcgc gcggcccggc ctggttgctg gcgtacagcg gtgtcgccag gttgttcgct
     5821 tcgtaggcgc ggaggatcgc cgggccgtcc gaggcgaagc cgtccacctg cgccgaccag
     5881 acgatcccat tgctcgtgcc gtcggccgag atgctctcca ccgtcccggg gaagccggag
     5941 acgatgttgc cctgagtgac cggggtctcg tccagcatca tcgtgttcgt cgccgggtcg
     6001 agcttcaggg cgaaggcctt gatcacgtcg cccactccct gaatgtagac cttgccgtcg
     6061 aagtaggccg gcgagctgta gatgaggccc tgggggttgg ccgtgaactc ctggataac
//
LOCUS       DATAQL010001456         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2747738, whole genome shotgun sequence.
ACCESSION   DATAQL010001456 DATAQL010000000
VERSION     DATAQL010001456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747738"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VHS97_25195"
     CDS             <1..257
                     /locus_tag="VHS97_25195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451574.1"
                     /translation="QARLWAERIMTGPSQSDPERLDSLYEIAFSRAPTLEEARACLAF
                     LDRQMQAGHARQGYDTQRQTLAWTALCHVLFNMKEFIFID"
     gene            297..1763
                     /locus_tag="VHS97_25200"
     CDS             297..1763
                     /locus_tag="VHS97_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3451575.1"
                     /translation="MHCRNFVEPGLDRRDLLLRCSAGFGALALAALFREPAFGARIAD
                     APTEHPNDATLAAANPLVSRPAHFPPKARSVIFLFMDGGPSQVDTFDPKPRLDREHGE
                     PIKVKTHPTQFNNVGKVMRCPWKFRNYGESGIPVSDLFPHVAQRVDELAIIRSMASNF
                     SEHTTANYFLHTGSGLQGRPSHGAWITYGLGSECQNLPAFVVLNGGLIPPGGIDCFNS
                     GFLPAAFQGSVFKPSATPVANIARAESTPELQREKLDLLRTLDEGVLGRMGRHDSLEA
                     AISNYELAFRMQSAIPELMSLHGESAATERLYGLDELYPPTRIFGRECLIARRLVERG
                     VRFIELLCPAVGGDRWDQHSGLKSGHENNARAVDKPIAALLTDLKGRGLLESTLVVWG
                     GEFGRTPMAQGSDGRDHNPYGFTMWMAGGGIKGGTIHGATDDYGYHAVENKVEIHDLH
                     ATILHLLGINHKRLTYRFGGRDMRLTDVHGEVVNAILA"
     gene            complement(1780..2088)
                     /locus_tag="VHS97_25205"
     CDS             complement(1780..2088)
                     /locus_tag="VHS97_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451576.1"
                     /translation="MAVPKLVSVDQPGASPLPISARLRSQLVYFMADATAAGVPPLGE
                     NEYWFTREEVAKWLDEGVFYLVSPLDTANMTEVELTEEQEAMLTWLDRNQVRHVRVVE
                     "
     gene            complement(2101..2718)
                     /locus_tag="VHS97_25210"
     CDS             complement(2101..2718)
                     /locus_tag="VHS97_25210"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HEX3451577.1"
                     /translation="MKRLSLTVVKVGGSLFDWPKFPHRFATFLQTLEADDGRERIVLI
                     AGGGPAADLIRLLDDLHSLGDETAHRLALLAMELSAHILTALLPKTVAIHDLDGLRSV
                     WSTGTIPILAPKLICDEIESSGQDLLPMNWNTTSDSIAARIAMHLAADRLILLKSAPL
                     PDGADRHEAARLGLVDPMFPVVTRSLARVEYVDLREDAAAPRLLP"
BASE COUNT          572 a          850 c          859 g          543 t
ORIGIN      
        1 gccaggctcg cctctgggcc gagcgcatta tgaccggacc cagccaatcg gaccccgagc
       61 ggctagacag cctttacgag atcgcctttt cccgagctcc cactttagag gaggccagag
      121 catgcctggc gtttctcgac cgccaaatgc aagccgggca cgccagacag gggtacgata
      181 cgcaaaggca aacgctcgcg tggaccgccc tttgccatgt tcttttcaac atgaaagagt
      241 ttatttttat tgactgagcc cagtgtcggt ccatctacag caatcgcgga accgccatgc
      301 attgccgaaa ctttgtcgaa cctggactgg accggcgcga ccttttattg cggtgcagtg
      361 cgggatttgg cgcgctggct cttgcggcgc tatttcgtga gccggccttt ggggcaagga
      421 tcgccgacgc gccgaccgag catccaaacg acgccacgct ggcggccgcc aacccgctcg
      481 tgtccaggcc cgcgcatttt ccaccgaaag cgcgcagcgt catttttctg ttcatggacg
      541 gcggaccgtc gcaggtcgac acgttcgacc ccaagcctcg gcttgaccgc gaacatggag
      601 agccaatcaa ggtcaagacg cacccgacgc agttcaacaa tgtcggcaag gtgatgcgct
      661 gtccttggaa gttccgcaat tatggcgaga gcgggatacc ggtcagcgac ttgttcccac
      721 atgtcgccca gcgcgtggac gagctggcaa tcatccgatc aatggcatcc aacttctccg
      781 agcatacgac ggccaactat tttctgcaca caggcagcgg gctgcagggt cggccaagcc
      841 atggcgcgtg gatcacatat ggtctcggga gcgagtgcca gaacctacct gcttttgtgg
      901 tgctaaacgg cggcttgatt ccaccagggg gcatcgattg cttcaacagc ggattcttgc
      961 cggcggcttt tcaagggtcg gtcttcaagc cgtcggcaac gccggtggcg aacatcgcca
     1021 gggcggaatc gacgcctgag ctccagcgcg aaaaactcga tctcctccgc acgctcgacg
     1081 aaggggtgct cggtcgcatg ggccgccacg acagcctgga agccgcaatc tccaactacg
     1141 agctagcctt tcggatgcag tcggccattc ccgagctcat gagcttgcac ggcgagtcgg
     1201 cggcgactga gcggctgtac ggtctagatg aattgtatcc accgacacgc atatttgggc
     1261 gtgagtgtct gattgctcga cggttggtcg agcgcggcgt gcggttcatt gagttgctct
     1321 gcccggccgt gggaggcgac cgttgggatc agcactcagg cctgaaatca gggcatgaaa
     1381 acaacgcacg cgccgtcgac aagccgatcg cggccctgct cacggaccta aaggggcgcg
     1441 gactcttgga gagcactctg gtcgtctggg gcggggagtt cggccgtacc ccgatggccc
     1501 agggatccga cggccgcgat cacaatccct acgggttcac catgtggatg gccggcggcg
     1561 gaatcaaggg ggggacaatc catggggcca ccgacgacta cggttatcat gccgtcgaaa
     1621 acaaggtcga gatccacgac ttgcacgcga cgatccttca tctcctggga ataaaccaca
     1681 agcggctaac ctaccgtttc ggcggtcgcg acatgcggct cacggacgtc catggcgaag
     1741 tcgtgaacgc gatcctggcc tgagaatcgc ggccatgcgt cactcgacca ctcgaacgtg
     1801 ccggacctgg ttgcgatcga gccaggtgag cattgcctct tgttcctcgg tgagctcgac
     1861 ctcggtcatg ttggccgtgt cgagaggcga gacgagataa aatacccctt cgtcgagcca
     1921 ttttgcgact tcttcacgcg taaaccagta ctcgttctcg cccagcggag ggacaccggc
     1981 ggctgtcgcg tcggccatga agtagacaag ctgcgatcgc agtctcgccg agattggtaa
     2041 cgggctcgct ccaggctgat ccaccgaaac aagctttggc acagccatgg cgtttcctta
     2101 ttacggcaac aatcgaggtg cggcggcatc ttctcgcagg tccacgtatt ctacccgcgc
     2161 gagtgatcga gtgacgaccg ggaacatcgg atcaaccaat cccagcctgg ccgcttcatg
     2221 acggtcggcg ccatccggca gaggcgcact cttgagcaag atgagtcgat ccgcggcgag
     2281 gtgcatggcg attcgggcgg cgatcgagtc ggaggtcgta ttccagttca tgggaagcag
     2341 gtcttggcca gagctttcga tttcatcgca gatcaatttc ggagccagaa tcggaatcgt
     2401 cccggtactc cagaccgagc gaaggccatc caggtcgtgg attgcgacgg ttttgggaag
     2461 aagtgcggta aggatgtgcg ccgacagctc catcgccaag agggccagtc ggtgtgcggt
     2521 ctcatcgccc aggctgtgaa gatcgtcgag caagcgtatc agatcggcgg ctgggccgcc
     2581 gccggcgatc agcacgatcc gctctctgcc gtcatcggcc tcgagcgtct ggagaaacgt
     2641 ggcaaagcga tgagggaact tgggccagtc aaagaggctt ccccccacct tgaccactgt
     2701 gagggatagg cgcttcatat cgggtcgtcc gtatgtcgat accgctccgc cgcgagtttg
     2761 accaaagcat gagcacaccc ggccgacgaa gcgactgccc cccaggcttc tttcaaactg
     2821 acga
//
LOCUS       DATAQL010001457         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4730398, whole genome shotgun sequence.
ACCESSION   DATAQL010001457 DATAQL010000000
VERSION     DATAQL010001457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4730398"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VHS97_25215"
     CDS             complement(<1..441)
                     /locus_tag="VHS97_25215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451578.1"
                     /translation="MTLRLVLVSMVAALGLTIPNRMDCKRFFDCAESRASSFLAGWDT
                     WRPGEGPGHRKSGATATRECELCRLARAQVALRAQKPADEVAIDSKAIAKVSSNDSEV
                     AVSKVQTAVPACTVSDTLITYEPIDVDVEICGGLALELNRAIEGI"
     gene            complement(611..3667)
                     /gene="treY"
                     /locus_tag="VHS97_25220"
     CDS             complement(611..3667)
                     /gene="treY"
                     /locus_tag="VHS97_25220"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX3451579.1"
                     /translation="MSPIEFADKLLSQAVGAAAARRPLPESTYRLQFHAGFTFRDALE
                     ITPYLSQLGITHCYASPYLKARPGSTHGYDIIDHGSLNPEIGTAEEYDEWVSALHEHG
                     LGQILDTVPNHMAVGTNDNPWWNDVLENGPASRYATYFDIAWRSSLRPELSDKVLLPV
                     LADLYGDVLEAGQFTLVYDDGAFSVDYAGRRFPIAPRSYDRILGHCLDELERGLGADD
                     TSFVELKSILTAVRNLPGSSETEPERVAERQREKEVIKRRLAALASISPPIREWIDGK
                     VAVFGGKPRDPASFALMDDLLEHQCYRLSYWRVGSDEINYRRFFDIDDLAALAMEREE
                     VFEAAHALIFRLVADGKVDGLRIDHPDGLFDPATYFRRLQDHALIARARHAFESDDAR
                     ESYDWKDVEQHIRDRLAAVDAPAGADGRADPPLYVAVEKILGSREQLVPTWPVHGTSG
                     YDFVNQLNGLFVDSGQVERVNRLYREIVPDAMPFSELVYRKKLLIVQASLASELHMLT
                     YQLDRLAQKSRRSRDFTFNTLRYALREVIACFPVYRSYVDSAGASAADRRYVESAVRM
                     ARIRNPLLSGRVFRFVRDMVLGDFPDLLGEHDRAEQQRFAGKFQQVTAPVMAKGVEDT
                     AFYVAQRLISLNEVGGDPARFGIRPEALHAFNQEQRLKWPFALCPLSTHDTKRSEDVR
                     ARINVLSEIPDDWGAAVSRYRELNRPHLQQVEDQVVPDPNEEYFLYQTLIGAWPLEPC
                     SADEYSIFVKRIQAYMEKALHEAKVHSSWINPDPDYDKGIQDFVGLILDDKLSGPFLT
                     EFRALERRVSQYGLFNSLSQTLLKLTSPGAADTYQGTELWDYSLVDPDNRRPVDYQVR
                     QEMLRQIRSSVAQSDDARELCRELLAARVDGRIKLYVHHKTLNLRKGQPGLFSTGEYA
                     PLFAAGSRTDHLFAFARRSGETVAIVAIPRLFTRLIPGADQVPLGQSVWQDTRLLLPA
                     EIMSADWRNLFTGERVAASRCDGQLSLPAAAVFADFPVAVLLNGRT"
     gene            complement(3705..>3891)
                     /locus_tag="VHS97_25225"
     CDS             complement(3705..>3891)
                     /locus_tag="VHS97_25225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451580.1"
                     /translation="TNPTAAAFEASGEYPLRERSLVVLVTRKPEDKGQIVTSTEARSA
                     LTGAKGSGSAPPGPSPI"
BASE COUNT          785 a         1132 c         1218 g          756 t
ORIGIN      
        1 aatcccttcg atggcacgat tgagctcaag cgcaagtcct ccgcagattt caacatccac
       61 gtcaatcggc tcgtacgtga taagggtgtc actgaccgtg catgccggca ccgcggtttg
      121 caccttggag actgcaacct ccgagtcatt ggacgagacc ttcgcgatcg ccttagagtc
      181 gatggcaact tcgtccgccg gcttttgtgc gcgcaaggcg acctgtgccc gcgccagccg
      241 gcagagctcg cattcgcggg ttgccgtcgc gcccgactta cgatggccag gtccctcacc
      301 gggtctccaa gtgtcccagc ccgcgaggaa tgagctcgcc cgcgattctg cgcagtcaaa
      361 gaagcgtttg cagtccattc ggttaggaat agtcaggccc agggcggcca ccatactgac
      421 caggaccagt cgcaacgtca tcgaaggatc tccggcacgt gtcgttttca tgggtctttg
      481 taaccgtccg gtacgtcacg gtgcatgtcc tagttgcatt ccacctgcgt gatctggtaa
      541 gagttatcga cttagcagac tggagacttc aagaagacaa gaaaatatag acaatatgaa
      601 tggcgcttca tcaggttcgc ccgttcagca gtacggcgac cgggaaatcg gcgaagaccg
      661 cggccgccgg cagcgagagc tggccgtcgc accgggaagc agcgacgcgt tcgccggtaa
      721 atagatttcg ccagtcggcg ctcatgatct cggcaggtag caagagccgc gtgtcttgcc
      781 acacgctttg gccgagtggc acttgatcgg cgccaggaat caagcgcgtg aagagccgcg
      841 gtatggcgac gatcgcgacg gtctcgcccg atcttcgcgc gaaggcaaag agatggtccg
      901 tacgactgcc agccgcgaaa agcggcgcat actcgcccgt actgaaaagt ccaggctggc
      961 ctttgcggag gttgagtgtc ttgtgatgga catagagctt gattcgcccg tcaacgcggg
     1021 ctgccaggag ctcgcggcag agttcccgtg catcgtctga ctgcgccaca gacgaccgga
     1081 tctgccgcag catttcctgc cgcacttgat agtccaccgg ccggcggtta tcgggatcca
     1141 cgaggctata gtcccagagt tccgtcccct gatacgtgtc cgcggcgcct ggtgaggtca
     1201 gtttgagcag cgtctgtgac agggaattga aaagaccgta ttggctgacc cgtcgttcca
     1261 gagcacggaa ctcggtcaga aagggaccgc tgagcttgtc atcgagaatc agcccgacaa
     1321 agtcctgaat gcccttgtca taatccggat cgggattgat ccagctcgaa tggaccttgg
     1381 cctcatgcaa cgccttttcc atgtaggcct gaatgcgttt gacgaagatc gaatactcgt
     1441 cggcggaaca aggctcgaga ggccaggcgc cgatcaatgt ctgataaagg aaatattcct
     1501 cattgggatc cgggaccact tgatcctcga cctgctgaag gtgcggccgg ttcaattcgc
     1561 ggtaccgcga aaccgcggca ccccagtcgt cgggaatctc ggagagcaca ttgattcgcg
     1621 cgcggacatc ctcgctgcgc ttggtgtcgt gggtcgataa tggacagagc gcgaacggcc
     1681 atttgagccg ctgctcttga ttgaacgcgt gcagcgcctc gggtcggatg ccaaaccgag
     1741 cggggtcgcc accgacttca ttgagcgaga tcaagcgctg ggcgacgtaa aaagctgtgt
     1801 cctcaactcc cttggccatg acgggagcgg tgacttgttg gaacttgccg gcgaaccgct
     1861 gttgctcggc ccgatcgtgc tcgccgagta agtcgggaaa atcaccgagt accatgtcgc
     1921 gaacgaaacg aaagacccgg ccgctcaaga gcgggttgcg tatccttgcc atccgcacgg
     1981 ccgactcgac atagcgccga tcggccgcgc tcgctccggc cgagtcgacg tacgagcgat
     2041 aaacaggaaa gcaggcaatc acttcgcgca gcgcgtatcg gagagtattg aaagtaaagt
     2101 cgcgcgagcg ccgcgacttc tgagccaatc ggtcgagctg atacgtcagc atgtgcagct
     2161 cactcgccag cgacgcctgc acgatgagca gcttcttgcg atagacaagt tctgagaacg
     2221 gcatggcgtc cggaacaatt tcgcggtaaa gcctgttgac gcgctcgacc tgtccgctat
     2281 ccacgaagag gccgttgagc tgattgacga agtcgtagcc actggtgcca tggaccggcc
     2341 aggttggaac gagttgctcg cgcgagccca ggatcttttc gacggcgacg tagagcggcg
     2401 gatcggcgcg accgtcggcg ccggctggcg catccacggc ggcgagacga tcgcgaatgt
     2461 gttgctccac atccttccag tcgtaagatt cgcgcgcgtc gtcggactcg aaagcgtgtc
     2521 gagctcgggc aatcaacgcg tggtcctgga gcctgcggaa gtaagtcgcc ggatcaaaaa
     2581 gtccgtcggg gtggtcgatg cgcagtccgt cgaccttgcc gtcggcaacc aggcgaaaga
     2641 tcagggcgtg cgccgcctcg aatacttcct cccgctccat ggccagcgcg gccaggtcgt
     2701 caatgtcgaa gaatcggcga tagttgattt cgtcggagcc cacacgccag taggaaagtc
     2761 ggtaacactg atgctcgagc aggtcatcca tgagcgcgaa actggcgggg tcgcggggtt
     2821 tgcctccgaa aacggccact ttgccgtcga tccactcgcg aatcggcggg cttatggagg
     2881 caagggccgc cagccgccgc ttgattactt ctttctcgcg ctgacgttcg gcaacccgtt
     2941 cgggctcggt ctctgagcta ccggggagat tacgcacggc cgtcaggata ctcttgagtt
     3001 cgacaaagct ggtgtcgtcc gctcccaggc cgcgctcgag ctcgtcgaga cagtggccca
     3061 ggattcggtc gtagctgcga ggggcgatgg gaaatcggcg gccggcgtag tcaacagaga
     3121 atgccccatc gtcgtaaacg agcgtgaact ggccggcctc gagcacatcg ccgtagagat
     3181 cggccaggac cggcagcagt accttgtcgg agagttctgg ccgcagagac gaccgccagg
     3241 cgatgtcgaa gtaagtggcg taccgggagg ccgggccgtt ctccagaaca tcgttccacc
     3301 acgggttatc gttggtgcca actgccatgt gattgggcac ggtatcgagt atctgaccca
     3361 gaccgtgctc gtggagagcg ctgacccact cgtcgtactc ttcggccgtg ccgatttccg
     3421 gattgagcga tccgtggtcg atgatgtcgt agccatgagt gctgccgggc cgggccttca
     3481 ggtagggtga cgcatagcag tgggtgatac ccagctggct caagtacggc gtgatctcga
     3541 gcgcgtcgcg gaaagtaaaa cccgcgtgaa actgcaagcg gtaggtcgat tcaggcaacg
     3601 gcctgcgcgc ggctgccgca ccgacggctt gcgagagcag cttgtcagcg aattcgatcg
     3661 ggctcatggt taactcgcgt gcttaagatc gctccgcgcc aagctcaaat cggagaaggg
     3721 cccggcggtg cgctgccgct gcctttcgcc ccagtaaggg cagatctggc ctcggtggac
     3781 gtgacgatct ggcccttgtc ttcgggtttg cgagtgacca ggacaaccag tgaacgttct
     3841 cgcaaaggat attcgccgga agcttcaaag gccgccgcgg ttgggtttgt c
//
LOCUS       DATAQL010001458         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1675354, whole genome shotgun sequence.
ACCESSION   DATAQL010001458 DATAQL010000000
VERSION     DATAQL010001458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675354"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VHS97_25230"
     CDS             complement(<1..464)
                     /locus_tag="VHS97_25230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451581.1"
                     /translation="MSEYAKKSMSRWGQAVACAAFSALLGLGGIWWWQGRPERYLARA
                     ENAFREDDPDEALAWLAVPESVKGTRERAQLLRARIAVEQGRLSEAALALDQVDPNGP
                     SAYDHAYWKGRMHYAARQPIPAITWFVAALKWRPDDADSHRWLAAASYDQGNR"
     gene            complement(687..2324)
                     /locus_tag="VHS97_25235"
     CDS             complement(687..2324)
                     /locus_tag="VHS97_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX3451582.1"
                     /translation="MNGLMMDWPLLIPNILRRAGQFFPEKEIVSRRCDGEVYRSTYGE
                     VQKRVHRLMNVLRKLGVNPGDRIATCAWNHQKHLELYFAVPALGAILHTVNFRLARDQ
                     LLYIINHAQDRLVFLDKSLGGILADLECELPSVEKYVLIDEGGPEPPALPQPSLDYEE
                     LLADASDQVEFPPLDEKMAAGLCYTSGTTGEPKGVLYSHRSTFLHALAGCAVDGGGMG
                     EKEVVLSVVPMFHVNAWGLPYSCTMAGAKQVLPGPQMIGKPIAELIESERVTSAAGVP
                     TIWNLLYQFLRTEADRPYDLSSLHTVLVGGAAASRTLVDSFEKDLGLPVLHAWGMTET
                     SPIGTISRLKSGMQDWTAEQKMAVRLKQGIPVMGVEVRILGEQGQELPWDGQHAGELV
                     VRGPWIASSYYNNPGADGAFTADGWFRTGDIAMVDPLGYVQITDRKKDLIKRKGEWIS
                     SVDMENLVLAHPGVLEAAVVARVDDVRDEVPVVFVVARPDLSTPINVQEIIDLIGTKF
                     ALWQLPKPEDIRFVTSLPKTGVGKLDKKVIRKMLVER"
     gene            2546..>4213
                     /locus_tag="VHS97_25240"
     CDS             2546..>4213
                     /locus_tag="VHS97_25240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3451583.1"
                     /translation="MASGPATTSAFYLSALLRSGTFAGLSDNELLERFASRRDEGDES
                     AELAFATLVARHGPMVLRVCRAVLADRHKAEDAFQATFLVFATRVSSIRRRQSVGSWL
                     HGVALRVAARARSGVAQRRRHERRRAEMTMRTTEADGDSPVLDDDRDCVLHEEIGRLP
                     ERFRSALVLCYLEGLTHEMAADQLCCPVGTIRSRLATARERLRRRLTHRGLAPAMFPV
                     GLSGSAVIPVADSAALSISLPVALVDTTVRGALRVSLGKSALAGIVSVEAVTLMEGVL
                     NNMVTTKLTLLTATILVAGLVTTGAGVAAYSALGRGEGLAGGPNPAQANFQEPVASPP
                     ASQKPAPRSLGPPPDVKEKLRRRSEDMVRTLLREYEVESVTSRKTMQNAKTAEERKAL
                     QAQPHPNPASYAGALLYEAEANPGTASAEDALIWIVTHLPYGSMAERAKEMIARDHVR
                     SEKIESLFVPSQTSMSGSTATERLFQEASAKNPSRKIQAIACFYLARFLENKASFIRL
                     GKMFDPTERENVELPIRREGWGHDYAERLYRLDPQEIEREAVQLYERL"
BASE COUNT          816 a         1305 c         1243 g          849 t
ORIGIN      
        1 cggttaccct gatcatagga cgccgccgcc agccatctgt ggctgtcggc atcgtctggc
       61 ctccatttca gggccgcgac aaaccaggtg atcgccggga tgggttgcct cgccgcatag
      121 tgcatccgcc ccttccagta cgcgtggtcg tatgcactcg gtccgttcgg gtcgacttga
      181 tccaacgcca aggctgcctc tgacagccgg ccttgctcca ccgcgatccg cgctcgtagc
      241 aactgagctc gctcgcgcgt tccctttact gactcaggta cggccagcca agccagtgcc
      301 tcgtctggat cgtcttcgcg gaaggcgttc tctgctcgag ccaggtaccg ttcaggtcgt
      361 ccttgccacc accaaatccc ccccagcccc agcaaagcgg aaaacgccgc gcacgcgacc
      421 gcctgtcccc acctcgacat cgactttttg gcgtattcgg acaatatcca cctcggtgag
      481 tcgggtggct catttcggtc attttgtctt gttcacgccc accaaagctc gaccatacct
      541 tgaatggaat cttgagctta gtgacggcga gctcgatctc gcggtacgcg gcaaaggcga
      601 tcagcaagat gatgctgaac caggccgacc ccagtttgaa ttgccagcct cgcaccccag
      661 tgcgagggcc aacgtcacaa tgctcgctac cgctcgacca gcatcttgcg tatcaccttc
      721 ttatcgagct ttcccacccc ggtcttgggg agtgaagtga cgaagcgaat atcctccggc
      781 ttgggcaatt gccacaatgc gaacttggtg cctatcaaat cgatgatttc ttgtacattg
      841 atgggtgtgc tcaggtcggg tcttgccact acgaatacga ccgggacctc gtcgcggaca
      901 tcgtctaccc gcgcgaccac ggcggcctcc agcactccgg ggtgagccag aacaaggttc
      961 tccatatcga cgctcgagat ccactccccc ttacgcttga tcagatcttt cttgcgatcg
     1021 gtgatttgta cgtagcccaa ggggtcgacc atcgcgatgt cgccggttcg gaaccagccg
     1081 tcggcggtga aagcgccgtc ggcgccggga ttgttgtagt agctactcgc gatccagggt
     1141 ccccgcacga cgagctcccc ggcgtgctgg ccgtcccagg ggagctcttg cccctgctcg
     1201 ccgagaatgc gcacctcgac ccccatgacc gggattccct gcttgagccg cacggccatt
     1261 ttttgctccg ctgtccagtc ttgcattccg gacttgagcc gcgagatcgt cccgatgggc
     1321 gaggtttccg tcatccccca agcgtgcagg acgggcagcc ccaggtcttt ttcaaagctg
     1381 tctaccaagg tccgcgaggc ggcggcccca cccaccaaca ccgtgtgcag gctggacagg
     1441 tcgtacggcc ggtccgcctc ggttctcaga aactgataga gcaaattcca gatcgtcggc
     1501 acacccgccg cagacgtcac ccgctcactt tcgatcagct cggcgatcgg cttgccgatc
     1561 atttgcggac ccggtaatac ctgcttggca cccgccatgg tgcaagaata aggcaatccc
     1621 cacgcgttga cgtggaacat gggcacgacc gacagcacga cctccttttc acccatgccg
     1681 ccgccatcga cggcgcaccc ggccagggca tggaggaagg tggaacggtg gctgtaaagt
     1741 acgcccttgg gctctccggt cgtccccgac gtgtaacaca gcccggcggc catcttctcg
     1801 tcgagcggcg gaaactcgac ctggtctgag gcatccgcca agagctcctc ataatccagt
     1861 gaaggttgcg gcagagccgg cggctcgggt cctccctcgt cgataaggac gtatttctca
     1921 acgctcggaa gctcgcactc gaggtcggcg agaatcccac cgagcgattt atcgagaaaa
     1981 acaagccggt cttgggcatg attgatgata tagagcaatt gatcgcgggc gagccgaaaa
     2041 ttgaccgtgt gcaggatcgc acccagagct gggacggcga agtagagctc caggtgtttt
     2101 tgatgattcc aggcgcaggt ggcgatacgg tcgccgggat tcacacccag tttgcgcaag
     2161 acgttcatga ggcggtgaac gcgcttttgc acttctccgt atgtcgaacg gtaaacttct
     2221 ccatcacatc gtcggctgac gatctctttc tccggaaaga actgaccggc ccggcgcaga
     2281 atgttcggga tcaagagcgg ccagtccatc atcagtccgt tcatcgtttg acctcgctcc
     2341 tcgaattgcc cagccgttcg attcgtgttg ttcgaacgta cgatgataac aagccgtcag
     2401 ccccgatttc atcaggcggc tgccaggagc gaatgggttc cgtagcatga agtcaacgat
     2461 ttttggaaaa tagatcgatt ggtcatgccc tctggagaca ctatccgagg acaacaatca
     2521 cgcgttttcc tcgggggctc ccgtagtggc cagcggacct gccaccactt ctgcctttta
     2581 cttatcagcc ctgctccggt ccggaacgtt tgccgggctg agcgataacg agcttctcga
     2641 acggtttgca tcccggcgcg acgaaggtga cgagtcggca gagctggcct tcgccacgct
     2701 cgtggcacgg cacggcccga tggtgttgcg ggtctgccgc gccgtgctcg ccgaccggca
     2761 caaggccgag gacgcatttc aagcgacttt cctcgtcttc gcaacacggg tgagctcgat
     2821 ccggcgacgt caatcggttg gatcttggtt gcacggagtg gcgttacgcg tggcggcccg
     2881 cgcacgatca ggcgtggcac aaaggcggcg acatgagcgg aggcgtgccg agatgacgat
     2941 gcgcaccaca gaggctgatg gcgatagccc cgttctcgat gacgacagag actgcgtcct
     3001 ccacgaggag atcggccgct tgcccgagcg gttccgatcg gccctggtgt tgtgctatct
     3061 cgaaggttta acccacgaaa tggccgccga ccagttgtgc tgcccggtcg ggacgattcg
     3121 gagccggctg gcgacggcac gggaacggtt gcgccggcga ctgactcatc gcggtttggc
     3181 acccgcgatg ttcccagtcg gtttgtcggg atcggccgtc attccggttg ccgattcggc
     3241 cgcattgtcc atctcgcttc cggtcgccct ggtcgatacc accgtgcgtg gtgcgctgcg
     3301 tgtgagcctg ggcaagagcg cactggccgg gattgtctcg gtagaggctg ttaccttgat
     3361 ggaaggggtt ttgaacaaca tggtgaccac caagctgact ctgttgacgg cgacgatcct
     3421 ggtcgccggg ctcgtgacca ccggcgcggg tgtggcggcc tattccgcgc tggggcgagg
     3481 cgaaggcctc gctggcgggc cgaatcccgc ccaagcgaac tttcaagaac cggtagcaag
     3541 tcccccggct tcccaaaagc cggcacctcg ctctttggga ccaccgcctg acgtgaagga
     3601 gaaactgcgt cgacgttctg aagatatggt gcgaaccttg ttgcgcgagt atgaggtgga
     3661 aagtgtgaca tctcgaaaaa cgatgcagaa cgcaaaaacc gccgaggaac gcaaggcatt
     3721 gcaagcccag ccacacccga atccagcgtc ttacgcggga gcattgcttt acgaggctga
     3781 ggcgaacccg gggacggcct ctgcggaaga cgccctgatc tggatcgtta cccatcttcc
     3841 ctacggctcg atggcagagc gggccaagga gatgatcgcc cgagaccatg tccggagcga
     3901 gaagatcgaa tccctgttcg ttccgtcgca gacgagcatg tcgggatcaa cggccactga
     3961 gcgcctcttt caagaggctt ccgccaagaa cccaagccga aaaattcagg cgattgcttg
     4021 cttctatctg gcgcgcttcc ttgaaaacaa ggcctccttc atccggctcg gcaagatgtt
     4081 cgatccaacg gaacgggaaa acgtggagtt gccgatccgg agggaaggct ggggccatga
     4141 ttacgccgag cggctttaca ggctggatcc acaagaaatc gagcgtgaag ccgtgcagtt
     4201 gtacgagcgg ctc
//
LOCUS       DATAQL010001459         4957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_610464, whole genome shotgun sequence.
ACCESSION   DATAQL010001459 DATAQL010000000
VERSION     DATAQL010001459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610464"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..915)
                     /locus_tag="VHS97_25245"
     CDS             complement(118..915)
                     /locus_tag="VHS97_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX3451584.1"
                     /translation="MKENPVKRALKAGKPQIGTWLTLGSIAAARFMARAGFPWLTVDL
                     EHTHTDIQTAAFMFGAIADAGCVPLARIPAGKHEYIKSVLDCGAMGIVAPMVMDRAEA
                     ELIVAACKYPPRGNRSIGGGMHALNFSATAEEYYSKADDEILVVIQTEHIQAVEIADE
                     IYSVPGIDAVFVGPNDLKYSMRAPDGTFADKQTFEATLTRIREAAQRQKLPCGLHVFS
                     AADATRRAREGWQFIAVVSELKMMLDGSADIVRQLNLQDAPGNVANY"
     gene            1262..2224
                     /locus_tag="VHS97_25250"
     CDS             1262..2224
                     /locus_tag="VHS97_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX3451585.1"
                     /translation="MEVIESLQGFVGENLFLLSPVDQAWQPTDYLPDLTTDDWAHNLS
                     QYRDTAQRVSDDLLVILVGNLVTEEALPNYSVSLEQIAHDPTGTGTAPWARWLRGWTA
                     EENRHGDLINAYLRLTGRVDMRAVERTVHHLIRNGFAPGWDHAYEGLVYAAFQERATR
                     ITHGNVGKLAAALGEENLALICRKIAADETRHETFYTRVIGQVMDRDPHGGMLAYNAM
                     LKGKIAMPGRLMFDGKDPDLFDHFGVVTQRNGAYTFQDYGEIITHLNGAWDIARRSVH
                     GEAAKAQEYICRQPERYMRLAGEIADRLAQQPHASFSWIHGRQV"
     gene            2357..3517
                     /locus_tag="VHS97_25255"
     CDS             2357..3517
                     /locus_tag="VHS97_25255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3451586.1"
                     /translation="MRVTLVTETYFPQVNGVSRTLRELVRVLTECGDEVQLIYPDYGE
                     AVDREKVHTVRSIVLPFYKELRLPLPPFSAVHRAIKSFQPDVLHIATEATLGLSVLRH
                     ALRRRLAVVSSFHTNFDQYSNHYHVGWAKMPIWRYLRWFHNRTRETYVPSETTIRELK
                     GLGFERLVLWKRGVDSTLFRPARPGRIDVRRKLGWAPNDVVISYVSRIAPEKNVDYLA
                     DALEIVASRRPDVRMLLVGDGPTRPALQRRVGSFAHFAGYRQGEDLADHYAASDIFAF
                     SSLTETFGNVVLEAMASGLPVVAVRAGGVGETVRSGTTGILVEPADPPARMAAALLES
                     IENREQRLTMAAAAREYALSQSWDAIMAGLRSRYEAAIDEHAGAAGASRVRI"
     gene            3614..4045
                     /locus_tag="VHS97_25260"
     CDS             3614..4045
                     /locus_tag="VHS97_25260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451587.1"
                     /translation="MQECSERPKRRVPGEDKLRGFSLQGRYDLLCARRDQGMAAIGLV
                     AQQVIDEEMPGRAVEPRVKMQGTLPSPNVQPRIFPDRDTAADVAPHLTQNDPLPSPLV
                     ANSQLLRGHEQIVADLKDDCRADRCCQCESRKGRAQQAPWL"
BASE COUNT          986 a         1498 c         1541 g          932 t
ORIGIN      
        1 gcgagcgcgg agtggcacgg gcttccagcc cgtggctgaa aatctcacgg gcaagatgcc
       61 cgtgccacgt acgccagccg agccgcttgc tcaacttact ctttcgcggt gccttagtta
      121 gtagttggca acgttacccg gtgcgtcttg cagattgagt tggcgtacga tatcggccga
      181 gccatcgagc atcattttga gctcgctcac gaccgcgatg aactgccagc cttcgcgggc
      241 acgacgcgtc gcgtcggcgg ctgagaacac gtgaaggccg cacggcagct tttgtcgttg
      301 cgcggcctcg cggatgcggg tcagagtggc ctcgaacgtt tgcttgtccg cgaatgttcc
      361 atcgggggct ctcattgagt atttcaagtc gttgggaccg acgaacacgg catcgatccc
      421 gggcacggag taaatctcgt cggcgatctc gaccgcttgg atgtgctcgg tttggatcac
      481 gaccaagatc tcatcatcgg ctttgctgta gtactcctcg gcagtcgcac tgaagttgag
      541 cgcgtgcatg ccgcctccga tcgatcggtt gccgcgggga gggtatttgc acgcggcgac
      601 gatcaactcg gcttcggccc tatccatgac catgggcgcg acgataccca tggcgccgca
      661 gtcaaggacg gacttgatgt attcgtgttt tcccgcgggg atgcgcgcca gcggtacgca
      721 gcccgcatcg gcgatcgcgc cgaacatgaa tgcggccgtc tgaatgtcgg tgtgcgtgtg
      781 ttccaggtcg acagtcagcc atggaaaacc cgccctcgcc atgaaccgag ccgccgcgat
      841 gctcccgagc gttagccagg tcccgatctg gggtttcccc gccttgagcg cccgcttgac
      901 cggattctct ttcaaaacag ttcctcgact ctattggggg aatgcgggcc tcgcgaacta
      961 tcacggccct gaagaagtga gtttcgcacg tccgggctgt gtcagcaaga gtttcagcgt
     1021 gttcatcgct gaggaattcg agtgaacggt tcaacacacg gaaacgaatg agaggcgatc
     1081 gccggactga aggctgtcct cttagagtgc tgattcgcag gggcggacgt cgaacggcat
     1141 tccgggcggt ccgccgagtc ctaatcaccg gctggataaa gtccagtcgc gtccgcgcgg
     1201 cgcaggtagc atgatgagtg agacatattc gagtgcggtc gagccagaaa ggcatccggg
     1261 aatggaagtg atcgagagtt tgcaagggtt tgtcggggag aacctcttcc ttctctcccc
     1321 cgttgatcag gcctggcaac cgaccgacta cctgcccgat ttgaccaccg atgattgggc
     1381 tcacaatctc tcacaatacc gggatacggc acagcgggtc tccgacgatc tgctggtgat
     1441 tctggtggga aatctcgtca ccgaagaggc cctgcccaac tattccgtct cgctcgagca
     1501 aatcgcccac gaccccactg ggacgggcac agcgccttgg gcgcgctggc tgagaggatg
     1561 gactgcggaa gaaaatcggc acggcgatct gatcaacgcg tacctcaggc tcaccggccg
     1621 agtggacatg cgcgcggttg agcggaccgt tcatcatttg atcagaaacg ggttcgcccc
     1681 gggctgggat catgcgtatg aggggctcgt ctacgcggca ttccaagagc gcgcgacgcg
     1741 gatcacccac gggaacgtgg gtaagcttgc cgctgcttta ggggaggaaa atctggccct
     1801 gatctgccgc aagatcgcgg ccgacgagac gcgacacgaa acgttttaca cccgcgtgat
     1861 cggtcaggtc atggaccggg atccgcatgg gggaatgctc gcctataacg cgatgctcaa
     1921 gggcaaaatc gcgatgcccg ggcgcttgat gttcgatggc aaagaccctg acctctttga
     1981 tcacttcgga gtagtcaccc agcgcaacgg ggcgtacacc ttccaagact acggcgagat
     2041 tatcacccac ctgaacggag catgggatat tgctcgccga tcggtccacg gcgaagccgc
     2101 caaagctcag gaatacattt gccggcagcc cgaacgctat atgcggcttg ctggcgagat
     2161 tgcggatcga ctggcccaac aaccccatgc gtccttctct tggatccatg gccggcaggt
     2221 ctagcgctca caccggctat gcgggcaccc tgccgtcgac gacgggatag tctgttccgc
     2281 tcggcgcgag tctggtaggg tctttcggtg tcaaacttgc cctccctttc accctggcgt
     2341 cgtctcgacg tacgatatgc gggtcacact ggtcacggaa acgtacttcc cccaggtcaa
     2401 tggtgtctcg cggacgctgc gtgaactggt tcgcgttttg acggagtgcg gcgacgaagt
     2461 tcaattgatc tatcccgatt acggcgaagc cgtggatcgc gaaaaggtcc atacggtccg
     2521 gtcgatcgtt ctgccgtttt acaaagagct gcgtcttccc ttgccaccgt ttagcgcggt
     2581 ccatcgggcg atcaagtcgt ttcagcctga tgtcctccat atcgccaccg aggcgacgct
     2641 cggcttgagc gtgctccggc acgcgctgcg tcgccgtctc gctgtcgtgt caagttttca
     2701 caccaatttc gaccagtaca gcaaccatta ccatgtcggc tgggcgaaaa tgccaatctg
     2761 gcgatatctg cgatggtttc ataaccgtac gcgagaaacg tacgtcccgt cggagacgac
     2821 aatacgagag ctcaagggcc ttggctttga gcgtctggtg ttgtggaagc gcggcgtcga
     2881 tagcacgctc tttcggcccg cgcggcccgg acgcatagac gtacgccgga agttgggctg
     2941 ggcgcccaac gatgtggtaa tcagttacgt gagtcggatc gcgcccgaga aaaacgtcga
     3001 ctatctcgcg gatgccctcg agatcgttgc gtcccggcgc ccggacgtac gaatgctctt
     3061 ggtaggcgac gggccgacac gaccggcctt gcagcggcga gtcggttcct ttgctcactt
     3121 tgccggatat cgccaagggg aggacttggc ggaccattac gccgcgtcgg acatcttcgc
     3181 cttctcgagc ctgactgaaa cttttggcaa tgtcgtgctc gaggcgatgg cgtcgggtct
     3241 gccggtcgtc gccgtccggg ccggcggcgt cggcgagacc gtccgatcgg gaaccacggg
     3301 tattctcgtc gaaccggcgg atccccccgc acgcatggcc gccgcactcc tcgagtcgat
     3361 cgaaaaccgg gagcaaaggc tcacgatggc tgccgcggcg agggagtatg cgctgagcca
     3421 aagctgggac gccatcatgg ccggacttcg aagccgatac gaggccgcga tcgacgagca
     3481 cgccggcgcc gcgggcgcga gccgcgtccg catttgaccg cgatggctcg accattggcg
     3541 cggtctcgaa gcgcccaaaa tcgtcgctct tgatccacag gccgggcatc aaccgcgatg
     3601 ggagataatg ctcgtgcaag aatgctctga gcgaccgaaa cggcgcgtac ccggtgaaga
     3661 taagctccgg gggttttcgt tgcagggccg ctatgatctc ctctgtgcgc ggcgcgatca
     3721 ggggatggcc gcgatcggcc tggtcgcgca gcaggttatc gacgaagaaa tgccgggtcg
     3781 ggctgtcgag ccgcgcgtaa aaatgcaggg gactttgcca tccccaaacg tacagccgag
     3841 gattttccca gatcgcgaca cggcggccga tgtcgcgccc catctgacgc agaacgaccc
     3901 actgccgtcc ccccttgtag cgaacagtca gctcttgagg ggacacgagc agatagtcgc
     3961 ggatctgaag gacgattgtc gcgccgatcg ctgctgccag tgcgagagtc gaaagggccg
     4021 cgcccaacag gctccatggc tttgatcgag gttttatagt gaccggcaga ggaaaacgtt
     4081 ccccgccggg tggctcagtt ggcgcccgtg cgatcgcgcg agcgagcagt gcagttgcgt
     4141 cggccagaca gaccgccacg gcaatcgctg tgccggcaat cggcaaaaga tagtagtgcg
     4201 gccagtacag acctgggagg gccacctggg cccatgccgc gagagtccat gcagcgacca
     4261 cgcgccgctc tagcgtttgc cgcggatgca gcatcagata tgcaagcgca ggaatggaga
     4321 ccagccatag gggccagcta ccggttcccc accagaccag gtagtcggtc gatccaaatg
     4381 gccaggggag ccggccggcc ggatcggcgt tgccggtgat cggccgcaga actgccagcg
     4441 gggcgttggg ctcaggcaag gtgtccgtcg cgagtgctct gccatactga aagatgtcca
     4501 ccgcggccca acgtccagcg ccttgagcga tcaggattat tgccgcggcc gccaagacca
     4561 ggaacaggcc catgccgagc gacgcgaggt tgatcaggca gcgcctgatt ttcgcctgcc
     4621 atccggcgtc ctgcgtccaa tctcgccagg cgacgcccac tacaaagacg agcaccgggg
     4681 caatagcaac ctgcttgacc agggccgctg cacccagaca cattcccgat ccgaagagcg
     4741 accaacggct ggcgcgatcc catccacgaa tcaatagaac aagcgacgcg accgcgaaac
     4801 agttgatgaa atgctcaaga ttggcaccat tgccgaacag ataaggatcc gtgctcaaga
     4861 agacgaatat cgctgcggcc acagacgccg accccggctc cgcgaggcgc agcgcgatcc
     4921 accacactag cacaattgtc gccaggacaa acggaat
//
LOCUS       DATAQL010001460         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3968385, whole genome shotgun sequence.
ACCESSION   DATAQL010001460 DATAQL010000000
VERSION     DATAQL010001460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3968385"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VHS97_25265"
     CDS             <1..777
                     /locus_tag="VHS97_25265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX3451588.1"
                     /translation="SPRLQQMLGSLQETTTNLREMSDSIRPAVESTVGHVEDMTRKLN
                     NNSPRIEKIILNVEGLTTEAEGYLLDNRENVKHSIASIRDLLASSNDIVAKDRAKVER
                     ILDGVEVTRTRADRLLYQADQIASQVSSVLTRSRADVERSVTNIRDATDWANRTVQKI
                     YANPIVVTPFYKPSHEDQRAQAVFDTAVVWTKGAQELHDTIKTIETMAARANTQQQQQ
                     ELTQLLRHARAMAETMNETSARLAEGFQKPGVKVRERTIR"
     gene            1027..1311
                     /locus_tag="VHS97_25270"
     CDS             1027..1311
                     /locus_tag="VHS97_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX3451589.1"
                     /translation="MEIYGAGGTQGPQPIYPRLAAFSLDAGQTVHAGAPRDHVEISPL
                     GQMLDGIGQLPEIRHERVDEIRRQLATGSYETPEKLELALDRMLDELIGW"
     gene            1625..>3017
                     /locus_tag="VHS97_25275"
     CDS             1625..>3017
                     /locus_tag="VHS97_25275"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX3451590.1"
                     /translation="MDSSHSSSVPPTGGSLPVERGVGGGSAEETMLSDLSAAEQNLIN
                     VLVKKGLLSSDQVRVAQVYGQEHNRDLRQSILELNLISPELLNQLAFERLSALATDNG
                     DDQVAGPSQAVAAAPPLSPNRTQHHRDVRKELQEKAAIVSLSELVNQILERACDCEAT
                     DIHFDPQENGLRVRYRIDGQLQDILFVEPAMATPVISRLKVISNLNIVERRHSQDGRI
                     TILHHNTPRDLRLATFPTIFGEKIVIRIHEVLTDVEGFTHLGMTAQQAETLDRLVSQP
                     YGAILVAGPVGAGKTSTLYNCLERINSPLRNVMTIEDPIEHRIPGANQCQISNPGEMG
                     FSEGLRAMLRQDPDVIMIGEIRDDETARIGIRAALTGVLVFSTLHGSDAPSTISNLYN
                     FGIPGYQLSSSLLAIVSQRLIRKICPYCRVAYSGDEKVFAALELDPDQHPELRMHRGL
                     GCPACFQTVYLGRT"
BASE COUNT          670 a          904 c          880 g          563 t
ORIGIN      
        1 agtccgcggc tccagcagat gctcggctcg cttcaggaga cgacgaccaa cctgcgtgaa
       61 atgagcgact cgatccgccc cgcggtcgaa tcgaccgtcg gccacgtgga agacatgacc
      121 cggaaactga ataacaattc accccgcatt gagaagatca ttctcaacgt cgagggactc
      181 acaaccgagg cggagggata tctgctcgac aatcgcgaga acgtgaaaca tagcatcgcg
      241 tcgatacgcg acctgttggc ctcgtccaac gatatcgtgg ccaaggatcg agccaaggtt
      301 gagcggatcc ttgatggcgt ggaggtgacg cggacccgcg ccgatcgtct cttgtatcag
      361 gctgaccaga tcgccagcca ggtgtcaagc gtcttgaccc gcagccgcgc ggacgtcgag
      421 cgttcggtta ccaatattcg cgatgcgaca gactgggcga acaggacggt tcagaaaatc
      481 tacgcaaacc cgatcgttgt gacccctttt tacaagccat cgcatgaaga tcagcgggca
      541 caggcggttt tcgacacggc cgtggtctgg accaaaggcg cgcaggagct gcatgataca
      601 atcaagacaa tcgagaccat ggccgctcgg gcaaatacac agcagcagca gcaggaactc
      661 actcaactcc tgcggcatgc gcgggccatg gcggaaacga tgaacgaaac ttccgcgcgg
      721 ttggccgagg ggttccaaaa gcctggcgtc aaagtgcggg agcggactat tcgatagatg
      781 tggcggcgaa acggggggtg ttgcgttcgc ggctttgatt aaactcgctc caaattccgg
      841 tcgatatgta gggtgtagat gctctctgta gggcatcgaa acgttgacgg agaccggagc
      901 gaggagatcc gactggcaaa cggtcggctc ccccctctcg cgtgcctccg tgggtaacga
      961 gtctgacaac acttggttct gccgatggcg gagtccatcg gccaaagcac ccgattggga
     1021 ggcgcaatgg aaatctacgg cgctggggga actcaaggcc ctcaacccat ttacccgcga
     1081 ctggccgcgt tcagcctgga tgccggtcaa acagtgcatg ccggggcgcc acgcgaccac
     1141 gtcgagatct ctcccttagg tcagatgctc gacgggattg gccagttgcc tgagatccgt
     1201 cacgagcgag tcgacgaaat tcgtcgtcag ctcgcaacgg gctcgtacga aaccccggaa
     1261 aagctcgagc tggccctcga tcgcatgctc gacgagctca tcggctggtg acgatcccca
     1321 aaaggggtct ccgagcggat gtgatgtaac gaaaatgata tggccgacgt gggggagcct
     1381 ctcaaggtat ccctgacgtc ggccgtttcg ttgaccggcg gcgatctttg cgtaaaaaac
     1441 tcctgcggtc gcgaaaatag cttggcaaga gggtcggaat tgggaaacaa tacccgaagc
     1501 ttcgccgccg cagcttacga cccacttcac gtttgacgac tcttgaattc aacaaccggg
     1561 tttttttaaa cgtgttcgcc ccgaggcaac cgagggcgaa ttgcccgccg aggaggtctc
     1621 gaccgtggat tcttcgcatt cctcatccgt ccctcccacg gggggatcgc tgccggtcga
     1681 gcgcggggtt ggcgggggct ccgccgagga aaccatgctt tccgatctgt ccgcggccga
     1741 acagaacctg atcaatgtcc tggtcaaaaa aggcctgctc tcgagcgacc aggttcgcgt
     1801 cgcgcaggtc tacggccaag agcacaaccg cgacttgcgg cagtcaattc ttgagctcaa
     1861 tcttatttct cccgaactgc tgaaccaact ggctttcgag cgtttgagcg cgctcgcgac
     1921 cgacaatgga gatgatcagg tggcgggacc aagtcaagcc gtcgcggccg cgccgccgct
     1981 ctcaccaaac cggacgcagc accaccgcga cgtgcgcaaa gagttacagg aaaaggccgc
     2041 gatcgtcagc ttatctgagc tggtcaacca gatcctagag cgcgcttgcg attgcgaggc
     2101 gaccgacatt catttcgatc cgcaggaaaa cggtttgaga gtccgctacc ggatcgacgg
     2161 ccagctccag gatatcttgt tcgtcgagcc ggcgatggcg acgccggtca tcagccggct
     2221 caaggttatc tccaacttga acatcgtcga gcggcggcac tcgcaggatg gccggatcac
     2281 cattctgcac cacaacacgc cgcgcgacct gcggctggcg acatttccaa cgatctttgg
     2341 cgagaagatc gtgatacgaa ttcacgaggt cctgactgac gtcgagggct ttacccacct
     2401 ggggatgacc gcccaacagg cggaaactct cgaccgtttg gtctctcaac catacggtgc
     2461 catccttgta gccgggccag tcggcgccgg caaaacctcg accctttaca actgcctcga
     2521 gcgaatcaac tccccgctca ggaacgtgat gacaatcgaa gatccgatcg aacatcgcat
     2581 tccaggtgct aaccaatgcc agatcagcaa cccgggcgag atgggcttca gcgaaggatt
     2641 gcgggcaatg ctccgccagg atcccgacgt catcatgatc ggcgagattc gcgatgacga
     2701 aactgcccgg atcggcatcc gcgccgccct caccggcgtc ttggttttca gtacgttgca
     2761 tggctcggat gcccctagca cgatcagtaa cctctacaac ttcggaatcc caggatatca
     2821 gctctccagc agcctactgg cgatcgtctc acaacggctg atccgcaaaa tctgcccata
     2881 ctgccgggtt gcctacagcg gtgacgaaaa ggtgtttgcg gccttggagc tcgatcccga
     2941 ccagcacccg gaactgcgaa tgcatcgagg gctgggctgc ccggcgtgct tccagaccgt
     3001 ctacttaggg cgaaccg
//
LOCUS       DATAQL010001461         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1983040, whole genome shotgun sequence.
ACCESSION   DATAQL010001461 DATAQL010000000
VERSION     DATAQL010001461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983040"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VHS97_25280"
     CDS             complement(<1..622)
                     /locus_tag="VHS97_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX3451591.1"
                     /translation="MRSKMALAEGISSERRSLDEVHGSVVVPTTHGTGQRLRRFFAFL
                     GPAYLVSVGYMDPGNWATDLEGGARFGYALIWVLLMSNVMAVLLQTLAARLGVVTGHD
                     LAQACRAEYSRLLNAVLWVLAEVAIAATDLAEILGTIIALKLLFGLPLLWGCLITAFD
                     TFLLLYLQRWGIRQMEAVILALVATIGACFLVQIILARPDMGAVGLG"
     gene            911..1396
                     /locus_tag="VHS97_25285"
     CDS             911..1396
                     /locus_tag="VHS97_25285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451592.1"
                     /translation="MTRTLLLTATILLSHALATSTQAQSTPYDARGFNSTAPGAWNYG
                     NLPNGSFGFDYAQPLPAGSLVMDQYGMVYATPTVESAPRAVVAQPAPITRTSGSTSNR
                     AKAKPRYQIPTGSLGWNGANGVILYSPAVRHETYDAGYARGPYGVVNYGHMSWGWPNG
                     Y"
     gene            1464..1943
                     /locus_tag="VHS97_25290"
     CDS             1464..1943
                     /locus_tag="VHS97_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451593.1"
                     /translation="MPVCSKTLAPATLFVAMLSAGVGANEARAQGVVGGGYSSSYRVS
                     DFLGAPGYYGMSYGFASYGMPQTYSVFSAFPGASYGSNYPPYGIQPGRFGVGLWRPGF
                     VAPGYVYGASYYQPWNSLSYHTFAVGSPYGSPANRIASPPPVGVYAPALGPRTLYGW"
     gene            2094..>3226
                     /locus_tag="VHS97_25295"
     CDS             2094..>3226
                     /locus_tag="VHS97_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX3451594.1"
                     /translation="MPGTIRRLIRGSRLRERVAPGGTIAVGIGSRGVHQIDLVARTIV
                     DTLRDMSYKPFIVAAMGSHGGATPEGQRELLAGYGVTSESMGVAVKTDMETVVLGTNP
                     VGLPIYFDKNAFEADGIILANRVKPHTDFRATHESGVLKMLVIGLGKRDGASQVHKLG
                     VRGLQEVLPAVGRFLVKSTPFALGLAIVENAQDVPAEIAAIEPETIFEIEPVLLERAR
                     GLMGRLPFDQIDLLVVGELGKNYSGAGMDPNVIGRLFVETQPDFDRPVVTRLVVLDVS
                     DESHGNIVGIGFADLTTDRLVSRMDQQSFRINTLTSCCLQRSRIPLTMATDREAIQAG
                     LETCWRIDPALARVVVIPNTLELQSLWVSKSLDDEVRAHPHLT"
BASE COUNT          663 a         1001 c          933 g          629 t
ORIGIN      
        1 aacccaggcc aaccgccccc atatcgggcc gcgccaggat aatctgaacc aggaaacagg
       61 cgccgatggt tgcgacgagc gccaggatga cggcctccat ctggcggatg ccccatcgtt
      121 gcagataaag aagcaagaag gtgtcgaacg cggtgatcaa gcaaccccac aagagcggta
      181 aaccgaaaag cagcttgagc gcgatgatcg ttcccagtat ttccgccaga tccgtcgcgg
      241 cgatggcaac ctcggccaaa acccacaaga cggcgttcag caggcgcgag tattcggcgc
      301 gacaagcctg cgccaggtcg tgcccagtca ccacccccaa tcgcgcggcc agggtctgca
      361 acagcacagc catgacgttc gacatcagaa ggacccagat cagcgcgtag ccgaatcgcg
      421 ccccgccctc cagatcggtc gcccagttac cggggtccat gtaacccaca ctgaccaggt
      481 aagccggacc gaggaacgca aagaagcgcc tgagccgctg ccccgtcccg tgcgtcgttg
      541 gcaccacgac cgagccgtgc acttcatcga gcgaacgacg ttccgaggag attccctcgg
      601 ccaaggccat cttcgacctc acagtcaaga gtaactattc tggtgcgcgc tcgactcgcc
      661 cacaccactc cccggagctc tcgaggccga acgccgagcc ggcactggtt ccattcttgt
      721 caagcaactg attcctactt taactcatct ttcggtttta tccaatgcca tccttgaaac
      781 cttaaaaaca attatttgga cacccaaaaa aagaaaatcg gtctcacttt gtccctatgc
      841 ctacccaaga gcgataatag aattaggacg acaagagcac cgcgctgatt tcacacctgg
      901 agaaactcgc atgacccgca cccttctatt gacagcgacg atcttgctgt cgcacgcctt
      961 ggcgacttcc acccaggcac agtcgacccc atacgatgcc cgcggattta attcgacggc
     1021 acctggggcc tggaactacg gcaacctgcc aaatggcagc ttcgggttcg attacgcgca
     1081 gccgttgccg gcgggaagtt tggtgatgga tcagtatggg atggtgtacg cgacaccgac
     1141 ggtcgaatca gcgcctcgag cggtggttgc gcaacctgca ccgatcacgc ggacgtctgg
     1201 ttccacctcc aatcgcgcca aggcaaagcc ccgctatcag atcccgactg gctctctggg
     1261 atggaacggc gcgaacggtg ttatccttta ctcaccggcg gttcgccacg aaacttatga
     1321 cgcggggtac gcgcgcggtc cttacggcgt ggtaaactac gggcatatgt cctggggttg
     1381 gcccaacggc tattgatcaa ccgagggacc catgcagtat tcgctaaacg agataacgga
     1441 ccgcacaaag aagggaaaaa ctcatgccag tctgctcgaa gacgctcgcg cccgcaacgc
     1501 tcttcgtggc gatgctgagc gcgggagtcg gtgcgaatga ggcgcgggct cagggtgttg
     1561 taggcggggg ctattcgagc tcgtaccggg tgagtgattt cctcggagcg cctgggtatt
     1621 acgggatgag ctacggtttc gccagctacg gcatgccgca gacatactcg gtcttttccg
     1681 cgttcccagg ggcgtcgtac ggttcgaact atccgcccta cggcatccag ccgggtcgtt
     1741 tcggcgtcgg tttgtggcga ccgggattcg tggctccggg ttacgtttat ggcgcctcct
     1801 actaccagcc gtggaactca ctttcctatc atacattcgc ggttggttcc ccctacggtt
     1861 cgccggccaa tcgcatcgca tcgcccccgc ccgtcggggt ttacgcaccc gcccttggcc
     1921 cgagaaccct ttacggttgg tgaagtttgg tacaatcatg cctttgtgcg atggaatgac
     1981 ggacttcttg gtcctgaagt ggacccgact ggcagcaaag gtcatgattc cctatggtat
     2041 ttccaccctt ggctcgagtc cggcaatcga tcccgcagcc gcgacttctt gacgtgccgg
     2101 gaacgattcg gcggttgata cgcggcagcc gcctgcgaga gagagttgcg cccggaggca
     2161 cgatcgccgt cggcatcggc agccgcggag tccaccagat cgacctggta gcgcggacga
     2221 tcgtcgatac gctccgggac atgagctaca aacctttcat cgtcgccgcg atggggagcc
     2281 atggcggcgc gaccccggaa ggccaacgcg agctgctggc cgggtacggg gtcacgtccg
     2341 agtccatggg cgttgccgtc aagacggaca tggaaaccgt cgtcctggga accaaccccg
     2401 tcggtttgcc gatctacttc gacaagaacg ccttcgaggc cgacggcatc atcctggcaa
     2461 accgggtcaa gccgcacact gactttcgcg caacccacga atccggcgtt ctcaagatgc
     2521 tggtgatcgg gctgggaaag cgcgatgggg ccagccaggt gcacaagcta ggcgttcgcg
     2581 gcctgcaaga ggttttgccg gctgtcgggc ggtttctggt caagagcact ccgttcgccc
     2641 tgggcctggc aatcgtagaa aacgcccagg acgtgcccgc cgaaattgcc gcgatcgagc
     2701 ctgagacaat ctttgaaatc gagccggtct tgctcgagcg tgcccgcggt ttgatgggcc
     2761 ggctgccatt cgaccagatc gaccttctgg tcgttggtga gctgggcaaa aattactccg
     2821 gagcgggcat ggatcccaac gtcatcggcc gactgttcgt cgagactcag cccgacttcg
     2881 accgtcccgt agtgacacgg ctggtcgtgc tcgacgtgtc ggacgaaagt cacggcaaca
     2941 tcgtcggaat cggcttcgcg gatctgacga ctgatcgtct ggtttcccga atggaccagc
     3001 aatcgtttcg gatcaatact cttacgtcct gctgtctgca gcgatcgcgg atcccgctca
     3061 caatggcgac cgaccgtgaa gcgatccagg ccgggttgga aacgtgctgg cgcatcgatc
     3121 ctgccctagc gcgggtggtc gtcattccca acactctcga gctgcagagt ctctgggttt
     3181 caaaatcttt ggatgacgaa gttcgcgctc atcctcacct cacctg
//
LOCUS       DATAQL010001462         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_913059, whole genome shotgun sequence.
ACCESSION   DATAQL010001462 DATAQL010000000
VERSION     DATAQL010001462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913059"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHS97_25300"
     CDS             <1..315
                     /locus_tag="VHS97_25300"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3451595.1"
                     /translation="HVACGRLAVQFVVHAIDKEGILATRAKFYREHRIHLDAAGKYGI
                     GVVTAGTLVANDGETPIGSLFVVDAEDRTTLDAFTRSDPYYVNGVWETVEIHAYRRKR
                     GS"
     gene            926..1894
                     /locus_tag="VHS97_25305"
     CDS             926..1894
                     /locus_tag="VHS97_25305"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3451596.1"
                     /translation="MRRREFIAGLGGAAVWPLGLDAQSPAIRRIGYVWIGARGTEVNA
                     AGLSQGLIDQGYLIGRDVAIEDRYADGYAERISALIGELLALKVDLLATPGTPIARAA
                     QRATSIVPIVCVTDNPIGFGLVASLSQPRGNITGLELVDSYYGEKSLQILQEIAPKRR
                     RIAVLKNPDNPLIVREIERLQGEAQVLGFDLTVFSVRSTDLEVNLAAINNRDFGGLVV
                     APDYFLEPLAPRIIAFAAERRLPAIYPFITAAEQGGLISYSADYFTIWRHAASYVDRI
                     FRGTRPADLPIEQAADLMLKVNLKTAKALGLNLPSTLVAGADQVIE"
BASE COUNT          416 a          560 c          620 g          435 t
ORIGIN      
        1 catgtcgcct gcgggaggct tgcggtgcaa ttcgtagttc atgctatcga caaggaaggc
       61 attctggcga cacgcgccaa attctatcgc gagcatcgaa tccatttgga cgcggcagga
      121 aaatatggga tcggcgtggt cactgccggc acattggtcg ccaatgatgg cgaaacgccc
      181 atcggcagcc tttttgtcgt cgatgcagaa gaccggacga cgctcgacgc cttcacccgc
      241 agcgatcctt attacgtcaa tggggtgtgg gaaacggtcg agatccacgc ctatcggagg
      301 aagcgcggct catgagggcg gtcgacggcc ttctcggccg agcagggccg cgaaagcgtt
      361 cgagttcagg caccaggggt cgcaacctaa cgccagtcga gaagctgatc cgtcgcggtc
      421 tccggaaata tggaacgacg acgtgtccga atagcatggt tgcttgcgag agtgccccgc
      481 gacgatcgga acagttcaga acttgccgtt caggccgagt ccgtgaggac cgagccccaa
      541 tgtggtgtcc ggcgcaaccg agagaccgcg ccgcggccca ttttgagatt gatcagattt
      601 attcgggtcg cttttcggca aacctgctga gttgtcggcc cgtggctttg gtgccggaca
      661 tggattcata attgtcggtt gacacgtcgt ctgggcgtga acgctggcta tgcttgcgag
      721 caatgccgcg gcggccatcc ctaaaattgt ggtagccgtt ctcatactgt cggtgcctcg
      781 tttgaatagg tatgctggca ttaaaagagg gaccgattgt gtcgcggaac cggaattgct
      841 gtggcagagc caaggccgaa acttaccgac ctgtttcaaa gcgcattgca tctcgtgata
      901 cgctgccgca ggcacggcga aaatgatgag acggcgcgaa ttcatcgcgg gtcttggcgg
      961 ggcggcggta tggccgttgg ggctcgacgc acaatcgccg gcaatccggc ggatcggcta
     1021 tgtctggatc ggtgctcgtg gaaccgaggt gaatgctgca gggctatcgc aaggccttat
     1081 cgatcaaggg taccttatcg gacgtgacgt tgcgatcgag gatcgatacg ccgatggata
     1141 cgccgagcgg atatcggccc tcattggtga gcttctcgcg ctcaaggtcg atcttctcgc
     1201 gacgcccgga acacccattg cccgcgcggc ccagcgcgca acttcgatag ttccgatcgt
     1261 atgcgtcacc gacaacccga tcggatttgg tctcgtggcc agcctatcgc aaccgagggg
     1321 caatatcacg ggcttggagc tggttgacag ctattacggc gaaaaatctt tacagatact
     1381 gcaagagatt gctccgaaac ggcgtcgaat cgcggtgttg aagaacccgg acaatccgct
     1441 gatcgttaga gaaattgagc gcttgcaagg agaggcacag gtgctcggct tcgatttgac
     1501 cgttttctcg gtgcggtcga ccgacctcga ggtcaatctt gccgcgatca acaacaggga
     1561 ctttggaggc cttgtcgttg cgcccgacta ctttcttgaa cctttggcac caagaatcat
     1621 agcgttcgct gctgagcgtc ggctgccggc catttatcca ttcatcacgg ctgcggagca
     1681 gggagggctg atctcgtatt cggccgacta ctttacaatt tggcgacatg cggcgagcta
     1741 cgtcgatcgc atcttccggg gtacgcgtcc ggcagatctg cccatcgagc aggcggctga
     1801 tctgatgctc aaggtcaacc tcaagacagc caaagcactc ggtcttaatc taccatctac
     1861 gcttgtcgcg ggtgccgacc aggtcatcga atgataggaa ttctatgcag gggcatgagt
     1921 ccgctgcgcg aagcccgtca atcgccccct ggtacccgac catgggcatt tttcgacagc
     1981 atggtcgcgg cgattggccc gggtcgtcac caagatcgtt gcggcgctcc a
//
LOCUS       DATAQL010001463         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1831902, whole genome shotgun sequence.
ACCESSION   DATAQL010001463 DATAQL010000000
VERSION     DATAQL010001463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831902"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1166)
                     /gene="msrP"
                     /locus_tag="VHS97_25310"
     CDS             complement(210..1166)
                     /gene="msrP"
                     /locus_tag="VHS97_25310"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX3451597.1"
                     /translation="MVIRRRRGWELRESAATPEAVFLGRRAVIKAIAAGPILAPALSS
                     ALPAEEDPSAGLYPAKRDPRYTLDRPLTDEKLATAYNNFYEFGSQKSIASDAQALKIR
                     PWTVKIDGLVEKPLTVDIDDLLKKMPLEERLYRHRCVEAWSMAVPWSGFPMAALIDFA
                     KPLGSAKYVRMETFLDPKTAPGQKEFWYPWPYVEGVTVAEAENELAFLVTGMYGKPVP
                     KQDGAPLRLALPWKYGFKSVKSIVHFSFTEKRPVSFWETVQGSEYGFWANVNPEVPHP
                     RWSQATERVLGTNERVPTLKWNGYGEFVAHLYDGLEKERLFV"
     gene            complement(1244..>2008)
                     /locus_tag="VHS97_25315"
     CDS             complement(1244..>2008)
                     /locus_tag="VHS97_25315"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451598.1"
                     /translation="TQEMVLWGQGDIHLQIAIDRLRTRHNLAVNGRRAGVPYKETIRQ
                     GAQQHSRFKRQSGGHGQFADVTIEVKPLPRGSGFTYTNSVVGGAIPRNYIPAVEEGVI
                     EALLHGPIGFPVVDVAVNLITGQFHAVDSSDQAFKTAGRQAVQEALPKCEPILLEPID
                     SVEVSVPNAFTARVQRLVSGRRGQILGYDAKPDWPGWDVVSAHLPQSELHDLIVELRS
                     LTMVVGSFAHQFDHMQELTGRPAEKVLASRTADAAQ"
BASE COUNT          349 a          649 c          617 g          393 t
ORIGIN      
        1 ggtcgcagcg gattgggggg cccgttgcgc aacggccggc cagtgccacc atgccatcgc
       61 gagcaggacc gccgcagcgg ccgggagcgt ccatttcttc atcgtcacca cctcgctgtt
      121 attgcccccg cggcgataca gaacaaaaca tgatcaaatt atgcaagcta aatttttccg
      181 gtcgcctctc gggataagtc tgccttccct cagacgaaga gccgctcctt ctccagcccg
      241 tcatagagat gggccacgaa ctcgccgtag ccattccatt tgagggtcgg cacgcgctcg
      301 tttgtgccga gcacccgctc cgtggcctgg ctccagcgcg ggtgcggaac ttcggggttg
      361 acgttcgccc agaacccgta ctcgctgccc tgcacggttt cccagaagga caccgggcgc
      421 ttttcggtga acgagaagtg gacgatcgac ttgactgatt tgaaaccgta tttccacggc
      481 aatgccaggc gcaacggcgc cccgtcctgc tttggaacgg gcttgccgta catgccggtc
      541 accagaaagg cgagctcgtt ttccgcctcg gcgaccgtga cgccctcgac atagggccag
      601 gggtaccaga attccttttg cccaggcgcg gtcttggggt cgagaaacgt ctccatccga
      661 acgtatttcg ccgagcccag aggcttcgcg aaatcgatca gggccgccat cgggaagccg
      721 ctccacggca ccgccatcga ccaggcctcg acgcagcgat ggcggtagag ccgctcttcg
      781 agcggcattt tcttaagcag atcgtcgatg tctacggtga gcggtttttc gaccagtccg
      841 tcgatcttga ctgtccacgg acggatcttc agcgcctggg cgtcggatgc aatcgatttt
      901 tgcgagccga actcgtaaaa attgttgtag gccgtggcca gcttttcatc ggtcagcggc
      961 cggtcgagcg tgtagcgcgg gtcgcgcttg gcgggataga gaccggccga gggatcttcc
     1021 tccgcaggaa gggccgaact caatgcgggc gcgaggatcg ggccggcagc gatcgctttg
     1081 atcaccgctc tgcgcccgag gaataccgcc tcgggtgtcg ccgcactctc gcgaagctcc
     1141 cagcctcttc ttcgccggat caccacgacc gcacctcgct accgttgcga cccagaagtt
     1201 ggatcggcct ccggcggcgc caagtcacgc tttcgcgatc ggcctactgc gcggcgtcgg
     1261 cagtgcgtga ggcgagcacc ttctccgccg gtctgccggt cagctcctgc atatggtcga
     1321 actgatgggc aaagctgccg accaccattg tcagcgaccg caactcgacg atcagatcat
     1381 gcaattcgct ttgcggcaga tgcgcgctga cgacgtccca gccgggccag tcgggcttgg
     1441 cgtcgtagcc gaggatctgg ccgcgccgtc ccgagaccaa tcgctgaacc cgggcggtga
     1501 acgcgttcgg cacggagacc tcgaccgagt cgatcggctc gagcaggatc ggctcgcatt
     1561 tcggcaatgc ttcctggacc gcctgccgtc ccgcggtttt gaatgcctga tccgaactgt
     1621 ccaccgcgtg gaattggccg gtgatcagat tgaccgcgac atcgacgacc ggaaacccaa
     1681 tcggtccgtg gagcagcgcc tcgatcaccc cttcctcaac cgccgggata tagttgcgcg
     1741 ggatggcccc gccgacgacg ctgttggtgt aggtaaagcc ggagccgcgc ggcaacggtt
     1801 tcacttcgat cgtcacatcg gcgaattgtc cgtgcccgcc gctctgacgc ttgaagcggg
     1861 aatgctgctg ggctccctgc cggatcgtct ccttgtaggg gaccccggcg cgcctgccgt
     1921 tgaccgcgag gttgtggcgg gtacgcaagc ggtcgatggc gatctgcaga tggatgtcgc
     1981 cttgccccca caagaccatt tcttgcgt
//
LOCUS       DATAQL010001464         7785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4424366, whole genome shotgun sequence.
ACCESSION   DATAQL010001464 DATAQL010000000
VERSION     DATAQL010001464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4424366"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VHS97_25320"
     CDS             complement(<1..1047)
                     /locus_tag="VHS97_25320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3451599.1"
                     /translation="MLPLDDFTANVAKSGLVSPDVVALIRAQLAPAPADDASLRLARQ
                     LIGGGWLTTYQARKLLAGATRGFFLGGYRLLRLLGEGGMGKVYLAVGNDRQNVAIKVL
                     PPRKALEDENVLQRFRREMELSQRCFHPNLARTLSVGNDGDVHYMVMEYIPGKSLYNL
                     VKSERAGPLRVPDAARLFLKLLDGLESAHHSGLIHRDIKPSNIMITPDGDVKLLDMGL
                     ARALDDETGLTRANTVLGTLDYASPEQLRDAAKADVRSDLYSVGCTLYFALAGRAPFE
                     GGDMINKIFRQRMEDPDPLETQARGVPASFAAIVRKLMSKKPQERHQTCAELRGDLAR
                     WTDHARARAILGAEA"
     gene            complement(1119..2093)
                     /locus_tag="VHS97_25325"
     CDS             complement(1119..2093)
                     /locus_tag="VHS97_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX3451600.1"
                     /translation="MSAVWRLINWLKSLLGLGDIAHVDDPATEEFATLSSFTLLPGQL
                     KLRVGVVSVKGNYRDHNEDNFFVPGRRSVRHDNNSESNTEMPSLTLEPSNLFIVADGM
                     GGQQAGEQASLMAVELIPRAIARRLSADENEPNRIKEVIRDAVAEVNQEILGSSGAIH
                     EYSNMGTTVVLAQFRFDRVFVAGIGDSRAYRLREGRLEQLTKDHSLADALLDAGTITA
                     DELATHKFKNVLYLYLGSKDARTGPEDVRVLDVRPGDRLLMASDGLTGVVPDKDLARV
                     LGTVDDPQKAAVELKNLALANDSKDNVTCLIIHAVAQHDSSTASRNGP"
     gene            complement(2174..2920)
                     /locus_tag="VHS97_25330"
     CDS             complement(2174..2920)
                     /locus_tag="VHS97_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3451601.1"
                     /translation="MAQTQDPELDLIAVGAHPDDVEIACGGTLARLAARGYSVGIVDL
                     TDGEPTPLSPGPEVRFAEAKRAAEILGATCRINLNMPNRRLFDSFENRVALAKVFRRY
                     RPKVVIGFGGKTVLASPDHYQAMLITDAAVFYSRLTKWDEYFDGLPTHTIVNQLSFPI
                     ALHGLDLPESSGYIVADIGSTLELKLEAIRAYRTQFPASKAGIFRAVESMNRYQGLTA
                     GFEAGEVFMTYRSVGIDDLMRWASPAHARP"
     gene            complement(3024..4289)
                     /gene="cbiE"
                     /locus_tag="VHS97_25335"
     CDS             complement(3024..4289)
                     /gene="cbiE"
                     /locus_tag="VHS97_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX3451602.1"
                     /translation="MSGARPKLVIVGIGDDGLAGLTERARKLVTSADLILGAPTSLSL
                     LESVPVRKQALEPDMSVALRQVREALAHTKPVLISYGDPLFYGVARYLCDRLGKDHFE
                     VVPHVSSMQLAFARIMESWEDAYLTSLSGRPIEAVIDRIRTAEKIGLFSSDDCPPGRL
                     ARELLDRGIDYFRAYVCENLGSPDERVTQALLGDLAGMEFSPLHVMILIREPNRPDRA
                     SRASRHRLFGNPDDAFAQSLPKRGLVTHAEVRAIALAQLDIRATSVVWDIGAGSGSVA
                     IEAAQLAHQGMVYAIEPEPSDIALIQANTEFFGVPNVQSILGRAPEILATLPEPEAIF
                     VGGTGRQVDLVLSTAYARLCQGGSMAVNVATIEGLSTAYHTLKALAGLVRVWNVSIAR
                     GIEQMDRLRFESIAPTFLLAVTKRDETGD"
     gene            complement(4500..5489)
                     /locus_tag="VHS97_25340"
     CDS             complement(4500..5489)
                     /locus_tag="VHS97_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3451603.1"
                     /translation="MSQILPDPRAGAEVADGEPSRQPCPAPANFRAEVARYDAQAEVS
                     RWSGPRYRMTYRILGEGPPLILAPGIASTYRTFALLLNRLGERFRTVLYDYPGEHSDD
                     AADLGHITHDHLVDDLFGLIEHLNIGRVFLVGFSFGSTVTLKALCREPRRFPRAVVQG
                     AFAFRTFTRGERWALKLGRLVPGNVERLPLRRRVLTYNSKSEFPSILDDRFSYYLEQN
                     GLTPIRSLAHRVELLTELDLRPILAQIPTELLLIQGNEDRIVARGYFEQLKAALPRAE
                     GLILPTVGHNPQLTHAEVTARLIGDWLLPCAPESCTSDQRSRAGCDAAGSPGM"
     gene            complement(5917..6753)
                     /gene="amrB"
                     /locus_tag="VHS97_25345"
     CDS             complement(5917..6753)
                     /gene="amrB"
                     /locus_tag="VHS97_25345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX3451604.1"
                     /translation="MPSLERPKLRLVVPRRVEHQGQPFVLLQDPTGVVAQPIILPFDG
                     FQHIVRHFDGQSTLNEIQARVLRQTGLFLALRELQDLVSRLDAAMILDGPTFAAFHQL
                     YRESGRRPAALAGRSYAATVRALRAQLEQFFVGSGGAGLPRDGHAGSATSFRGILSPH
                     IDFQRGGPVYTFSYKELVERSQADTFVVLGVAHQYCRRRFALTRKDFETPLGLVPTDR
                     SYVDRIAALAGHELFDDELSHRTEHSIEFQVVFLQYLLGAKRDFSIVPILVGSFHDLM
                     ER"
     gene            7368..>7785
                     /locus_tag="VHS97_25350"
     CDS             7368..>7785
                     /locus_tag="VHS97_25350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451605.1"
                     /translation="MPFPRRIWIAPLVALFSAGFTLAVAQDRGKVAQPAQRQQQPAAA
                     QPAMNMDQLLQLWAGQSAKLETLELDIYRIDEQKAWGDVEHYLGHAAFKAPQLAFLDF
                     RKVSLQEQADPKDKKKKVVVPVKKNNKIESHPYETII"
BASE COUNT         1666 a         2398 c         2300 g         1421 t
ORIGIN      
        1 ggcttccgcg ccgaggatgg cacgcgcccg ggcgtgatcg gtccaccgag ccaggtcgcc
       61 gcgcagctcg gcacaagtct ggtggcgctc ctggggtttc ttgctcatga gtttgcgaac
      121 aatcgccgcg aatgatgccg gtacgccgcg agcctgggtc tcgagtggat cgggatcctc
      181 catccgttgc cggaagatct tgttgatcat atcgcccccc tcgaacggcg cacggcccgc
      241 cagtgcgaaa tagagggtgc agccaacact gtagaggtcg cttctcacgt cggctttggc
      301 ggcgtcgcgc agctgttcgg gactcgcata atcgagcgtg cccaacactg tgttggctcg
      361 ggtcagcccg gtttcgtcgt ccaaggcccg tgccagaccc atatcgagca gctttacgtc
      421 cccgtcgggc gtgatcatga tgttggacgg ttttatatcg cgatggatca acccggaatg
      481 gtgagccgac tcgagaccgt cgagcagctt caagaataac cgggccgcgt cggggacccg
      541 gagcggaccg gctcgctcgc tcttgactaa attgtagagg cttttgccgg ggatgtactc
      601 catgaccatg tagtgaacat cgccgtcgtt gcccaccgaa agcgtgcgcg ccaggttagg
      661 atggaaacag cgttgtgaga gctccatttc acgtcgaaac cgctgcaaga cgttctcgtc
      721 ctcgagcgct ttccgagggg gaaggacctt gatcgcaacg ttctggcggt cgttgccgac
      781 cgcgaggtaa accttcccca tgcccccttc gcccaatagg cggagcagac gataaccgcc
      841 caagaaaaac ccgcgtgtag cccccgcaag gagctttctc gcctgatagg tggtgagcca
      901 accccctccg atcaattgcc tcgccagtcg gagcgacgca tcgtcggcgg gcgctggcgc
      961 gagctgggcg cggatcaaag ccaccacgtc gggcgagacg agtcccgact tggcgacatt
     1021 ggccgtgaag tcgtccagcg ggagcattgt ccacacactt cggtcatttg agcgcagtcc
     1081 gcaacaccaa ccgagttttt tcggaatgcg cccgactgtc aagggccgtt gcgagaagcg
     1141 gtcgatgagt catgctgggc gacagcatgg atgatcaggc aggtcacatt gtccttcgag
     1201 tcgtttgcaa gagccaggtt tttgagctcg accgcggctt tttgggggtc gtcaacggtt
     1261 cccagaactc tggccaggtc tttgtcgggc acgacgccgg tgaggccgtc gctggccatc
     1321 aaaagtcggt cgcccgggcg gacgtcgagc acccgcacgt cttctggacc ggtgcgcgca
     1381 tccttgcttc ccagatagag ataaaggacg ttcttgaact tatgcgtggc gagctcgtcg
     1441 gccgtgatcg tgcccgcatc aaggagggca tcagcgagcg agtggtcttt ggtgagttgt
     1501 tccaaacgac cctcgcggag gcgataggcg cgggaatcgc cgatacccgc cacgaaaacc
     1561 ctatcgaatc gaaactgggc gagcaccacc gtggtaccca tattggagta ctcgtggatc
     1621 gcgcccgagc tgcccaggat ctcttgattg acttcggcga ccgcgtcccg gatcacttcc
     1681 ttgatccggt tgggctcgtt ttcatcggcg gacaaccggc gggcgatcgc cctggggatc
     1741 agctcgacgg ccatcaggct cgcctgctct ccggcctgct gcccacccat gccgtcagcc
     1801 acgatgaaca gattggaagg ctcgagagtg agcgacggca tttcagtgtt gctttccgag
     1861 ttattgtcgt gccgcaccga tcgtcggccg ggaacgaaga aattgtcttc gttatggtcc
     1921 cgatagttgc ctttgaccga caccacgcct accctcaatt tcaattgacc cggcaagagc
     1981 gtaaacgagg aaagagtggc gaactcctcg gtggccgggt cgtcgacatg agcaatgtcc
     2041 cccaggccca agaggctttt caaccagttg atcaatcgcc aaaccgcgct cacaagacgc
     2101 tcacccttcc ccaagaaccc cgattccccg cacgatcgca tcggcaaagc gattgcgatc
     2161 aaatcacgga aaatcacgga cgtgcgtgcg ccgggctggc ccagcgcatc aggtcgtcga
     2221 taccgaccga tcgataggtc atgaacacct ctcccgcctc gaatcccgcg gtgagccctt
     2281 gatagcgatt catcgactcg acggcccgaa atattcccgc cttgctggcc ggaaactggg
     2341 tccggtatgc acgaatcgcc tcgagcttca gttccagggt cgatcctatg tcggcaacaa
     2401 tgtaaccgga tgactcgggt aagtccaacc cgtgcaacgc gattggaaaa cttagctggt
     2461 tgacgatggt gtgggtcggc agaccgtcga agtactcgtc ccacttcgtc aggcgactgt
     2521 agaaaaccgc ggcgtcggtg atcaacatgg cctggtaatg gtcgggagag gcaagcacgg
     2581 tcttgccgcc gaaaccaatg acgaccttgg gtcggtaccg gcggaacacc ttggcaagcg
     2641 cgactcgatt ctcgaacgag tcaaacagcc gccggttggg catgttgagg ttgatcctgc
     2701 acgttgcacc caggatctcc gcagcccgct tcgcctcggc gaaccgcacc tcgggccctg
     2761 gtgaaagcgg ggtgggctcg ccatcggtca gatcgacgat tcccacagaa taaccccgcg
     2821 cagcgagccg cgccagggtt cccccgcagg cgatttcgac gtcatcgggg tgggcgccga
     2881 ccgcaatcag gtcgagctcc gggtcctgcg tctgcgccat gcgtctttaa cacctcgcga
     2941 atctgcgacc atcacccgga atcgatccaa tatataccgt gaaatcgatt cttcctgtcc
     3001 aacccatggg cggaaactat tgctcaatcg cccgtttcat ctcgtttggt caccgccagc
     3061 aaaaatgtcg gtgcgatcga ctcgaaccgc agccggtcca tttgctcgat ccccctggca
     3121 atcgatacat tccagacgcg caccaggccg gcaagtgcct tcaacgtatg ataggctgtc
     3181 gagagcccct cgattgtcgc cacgttgacc gccatgctcc ccccctggca aagccgcgca
     3241 taggccgtac tcaagaccag gtcgacctgc cgccccgtcc ctccgacaaa gatggcctcg
     3301 ggctcgggga gagtcgccag gatctccggg gcgcgtccca agatcgactg cacgttgggc
     3361 acaccgaaaa actcggtgtt cgcctgaatg agcgcgatgt ccgacggctc aggctcgatc
     3421 gcatagacca tcccctggtg agccagctgc gccgcctcga tcgcgaccga gcccgacccc
     3481 gcgccgatgt cccagacgac cgacgtggca cgaatatcga gctgagccag ggcgatggcg
     3541 cgaacctcgg cgtgggtgac cagtccccgc tttggaagcg actgcgcaaa ggcatcgtcg
     3601 ggattgccga acaagcggtg gcggctggcg cggctcgccc ggtcgggacg attcggctcg
     3661 cggatcaaaa tcatgacatg cagcggactg aattccatgc ccgctaaatc gcccaaaaga
     3721 gcctgtgtca cgcgctcatc gggtgaaccc agattttcac acacatacgc tcgaaagtag
     3781 tcgattccgc ggtcgagcaa ctctctggcc agacgccccg gaggacagtc atcactcgaa
     3841 aacagaccga tcttctcggc ggtcctgatc cgatcgataa ccgcctcgat cggccggccg
     3901 gaaaggctcg tcagatacgc gtcctcccaa ctttccatga ttctggcaaa ggcaagctgc
     3961 atgctgctga catgcggcac aacctcgaag tggtccttgc ccagacggtc gcagagatag
     4021 cgggcgacac catagaaaag aggatcgcca taggagatca ggaccggctt ggtatgcgca
     4081 agcgcctcgc gtacctgccg caaggccacg ctcatatcgg gctcaagagc ctgcttgcgc
     4141 accggaactg actcgagcag cgacagactc gtcggcgccc ccagaatcag gtcagcggac
     4201 gtgaccagtt tgcgcgcccg ctcggtcaat ccggcgagtc catcatcgcc gatccccacg
     4261 atcaccaact taggtcgggc accgctcaac gtcgccgtcc cagattgtcc cagcctcaat
     4321 gccgctagct caatttcacc aaggccgtgc gtagtcgcat tgtactccgc gagcgactcg
     4381 caatccaact ggtaagagac aatcaatcca gaacatctga ttgccggagg cccgtgttcc
     4441 ctgagccagt cgacttgccc acgaaagtcg tctcccgact ctgccatcat cacttgcatt
     4501 cacatgcccg gcgatccggc ggcgtcgcag ccagctcgcg atctttgatc gctcgtgcag
     4561 ctttcgggag cgcaagggag cagccaatcg ccgatcagcc tggccgtgac ctcggcatgg
     4621 gtcaactggg ggttatggcc cacggtcggc aggatcaagc cctctgcccg cggcagcgcc
     4681 gccttcaact gctcgaaata accgcgggcg acgatgcggt cttcatttcc ttgaatcaaa
     4741 aggagctcgg tgggaatttg tgcgagaatg ggccgcaaat cgagttccgt caggagctcg
     4801 actcgatgag cgagcgaccg gatcggcgtc agaccgtttt gctcgagata atagctaaag
     4861 cggtcgtcga gaatcgatgg gaactcactc ttgctgttgt acgtaagcac gcgccggcgc
     4921 aagggtagtc gttcgacgtt ccccggaacc agtcgcccca acttgagcgc ccaccgctcg
     4981 ccccgagtga aagtgcgaaa ggcaaacgcg ccttgcacca cggccctagg aaagcggcgc
     5041 ggctcgcgac agagcgcctt gagcgtgacc gtcgagccga acgaaaaacc gaccagaaac
     5101 acacgaccga tgttgagatg ttcgatcaat ccgaatagat cgtcgaccag atgatcgtgc
     5161 gtgatgtggc caagatcggc ggcgtcgtcg gagtgctcgc ccgggtagtc gtacagcaca
     5221 gtccggaacc gctcaccgag ccggttgagc aacaaggcga aggtgcgata ggttgacgcg
     5281 attcctggcg ccaggatgag cggaggtccc tctcccagaa tccgataggt cattcgatag
     5341 cgaggaccgg accagcggga tacctcggcc tgcgcgtcat aacgagcgac ttccgcgcga
     5401 aagttcgccg gagcagggca tggctggcga ctgggttcgc catcagcaac ctctgccccg
     5461 gcacgcgggt caggcaggat ttgcgacatg agactcggcc gttgtcagag gagggtcgtc
     5521 ggcatggaat atcatgctcg caaagctcac gcagcatgtc cgtcggtcgt cgagagcctg
     5581 aatatacctg agcaactgac ctcgtgccgg cccgatcgca tgcaacatgg tgtatgtcgc
     5641 ggcgaggccg cagacccgat agcggttgcc cacgcccgcg acagtctgaa accatccgct
     5701 cgggtcgcac gccgcggccc gctcgagcag gcggtcgtca aagagcctga cctgctcttg
     5761 aaaccccggg tcgacaggat gaggatcgcc aaactcaggc ccgacgtggc tcaggtcgat
     5821 gccaccgata taggcgactc gcttgccgtg agcaaactcg gcggctcgca gcgcctcgat
     5881 gaatcggttt acttctgggt cctcgatggg atcgattcag cgctccatca agtcgtgaaa
     5941 cgagccgacc agaattggca cgatcgaaaa gtcgcgtttg gctcccagca ggtactggag
     6001 aaacacgacc tgaaactcga tcgagtgttc ggtccgatgt gatagctcgt catcgaaaag
     6061 ctcgtgcccc gccagcgcag caatcctgtc gacgtacgag cggtccgtcg gcaccagtcc
     6121 cagcggggtc tcgaaatcct tgcgagtgag ggcgaaacgg cgccggcagt attgatgggc
     6181 gacacccaac accacaaagg tatcggcctg cgaccgttcc acaagctctt tgtacgaaaa
     6241 ggtatagaca gggccgccgc gctggaaatc aatgtgcggg ctcaagatcc cgcgaaagct
     6301 cgtcgccgac cctgcgtgcc cgtcacgcgg cagtccggca ccaccggacc caacgaaaaa
     6361 ctgttcgagc tgggcccgca gcgcacgtac tgtggcagcg tacgagcgac cagcaagcgc
     6421 ggcgggacgc cgacccgatt cgcgatacaa ctggtgaaac gccgcgaacg tcggcccatc
     6481 caagatcatg gcggcatcca gtcggcttac cagatcttgc agctcacgaa gagccagaaa
     6541 aagaccggtc tggcgaagca cccgagcctg gatctcgttc aaggtcgact gaccgtcgaa
     6601 atggcgaacg atatgctgaa acccatcgaa cggcaggatg atcggctgtg cgacaacacc
     6661 ggtcggatct tgcagcaata caaagggttg cccttggtgc tctacgcgcc taggcactac
     6721 cagccgaagt ttcggtcgtt cgagactcgg catgccagaa accaggccct cgcaacgcca
     6781 taagaccgta ttgaaatgcc cacgcctgcg cgcgaggatc atacgatcat gttagccgag
     6841 agccgccagc cgtcaagccg tatggcgcgg accacgggga agactcgaga tcaacggcag
     6901 tggccacgcc ggtggagcaa tctcgcggat cggttggggt cgcatcgcaa tgcctccctc
     6961 atcacacgca gacgtcaatg aaataggaat cgagaccgcc aacaggacag agcaaaaacg
     7021 atgccaaacc ttgaaggatt gtccgaagtc tctatttcac cgccagttca acttgattag
     7081 tcggcaggat tttgcgccgc gaagcggtcg ggaaggtcat tgcgacatcg cttccgcctt
     7141 ttccggtagc ggcaatgcca ccaagatgat cagcggaaga agatcgcgag ggctaaagat
     7201 gaagcgagcc ggcaacgggt gcaagggccc cgcatgcggc tgctgcactg gtggtcgtgg
     7261 ccgaactatg gtagttttcg cgcccgcaag ggtctttaag gcgaccgcga gcacgattgc
     7321 gcgcgagtca taggggctgg gacggatagc agggaggacg attacccatg ccatttccaa
     7381 gaaggatctg gatcgcaccg ctggtcgcgt tattctccgc gggtttcacg ctcgccgtcg
     7441 cccaggatcg gggcaaagtt gctcagccgg cgcaacggca gcaacaaccg gcggcagccc
     7501 agccagccat gaatatggac cagctcctgc agctttgggc agggcaaagc gcgaagctcg
     7561 agactcttga gctcgatatc taccggatcg atgagcaaaa agcctggggc gacgtggaac
     7621 attatctggg gcacgccgct tttaaagctc cgcaactcgc gtttctcgac tttcgcaaag
     7681 tcagtttgca agaacaggcc gatcccaagg acaaaaaaaa gaaagtcgtg gtgcccgtca
     7741 aaaaaaacaa taagatcgaa tcgcacccgt acgaaaccat tattt
//
LOCUS       DATAQL010001465         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1983474, whole genome shotgun sequence.
ACCESSION   DATAQL010001465 DATAQL010000000
VERSION     DATAQL010001465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983474"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VHS97_25355"
     CDS             <1..904
                     /locus_tag="VHS97_25355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451606.1"
                     /translation="TQASAGATDRHMPIVIALIAIAELAWLAWFLIVPLPNANNVGVQ
                     PEKAVRRGLLLLKTFPQVVPDTPFRDSLLGKGLKELSHLENLPQRLPIILTAGLIAAA
                     AVGLGDLVIRGLRAHVALSIAERIAIDYGLGAGLLGVITLLIGRLGQLDPWLFRVGLG
                     LLAVAGLAGSAVWRGGWRRIDASSWPGALLFAPFVLVMILGSMLPSIDFDVLEYHLQG
                     PKEYYQAGRIAFLPHNVYTNMPFGVEMLHLMGMEVLGDFWWGGLAGQLLIALFAPAAA
                     VLIAATASRSGSARAAWVAAIVYL"
     gene            929..2083
                     /locus_tag="VHS97_25360"
     CDS             929..2083
                     /locus_tag="VHS97_25360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451607.1"
                     /translation="MIAYVEGPLCFYHAALLWAVVRGWADRSLPRRRIWFAIGLLAGC
                     AMGCKYPALISAVVPFGVVALWDCRRSRSLEPLLFYVLGWGIVMGPWLVKNVIDTGNP
                     VYPLADSVFHGRFWDQALEQKWSAAHGRRPVSYQELIGSLIDVAGRSDWQSPLYVALA
                     PLALLRPGSRRLAMALWGLVAYLFATWWLLTHRLDRFWLPLLPALAILAGLGADWVRN
                     RGWTLVLGVIMAIGLLANFTYITTALAGLNEWTGDLVFLRRDIPRRWNAPLARLDTEL
                     PANARPLLVGQAAVFHLTHPVAYNTVFNPETIELLAKDKNNDEFHDSLRQRKLSHIYV
                     DWKEIKRHRQPGGYGFTDFVTPSRFAQWVADGVLERPLLVGPEQELYQVR"
     gene            2133..>2603
                     /locus_tag="VHS97_25365"
     CDS             2133..>2603
                     /locus_tag="VHS97_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3451608.1"
                     /translation="MDDLTIVTGGAGFIGSHLVAHLVNSGQQVRVIERPGVAVDHLPR
                     IAQLHFADIRDRAALEPALAGGKWVYHLAANPHLWVRDRREFQEVNFEGTKNVLDVSL
                     KAGAQRILHTSTESILTKAEVTGPIDENIEVSLSDAVGPYCRSKLLAERYAFSLA"
BASE COUNT          445 a          768 c          786 g          604 t
ORIGIN      
        1 aacccaggca tccgccggtg ccaccgatcg tcacatgccg atcgtgatcg ccttgatcgc
       61 catcgccgag cttgcttggc tcgcctggtt cttgatcgtc ccgcttccca acgccaacaa
      121 cgtgggagta cagccggaga aagcggtgcg gcggggtttg ctcttgctga agacgttccc
      181 acaagtagtg cccgacacac cgtttcgtga ctcactgctc ggcaagggac tcaaagagct
      241 cagccatctc gaaaacttgc cacaacgcct tccgattatc ctcaccgcgg gcttgatcgc
      301 ggctgcggcc gtggggctgg gcgatctggt gatccgcggc cttcgcgctc atgtggcttt
      361 gagcattgca gagcgaatcg caatcgacta tggcttgggc gccggactgc tgggggtcat
      421 cacgcttctg atcgggcggc tcggccagct tgatccgtgg ttattccgtg tcggactcgg
      481 gctgttggcg gtcgccggac ttgcaggttc cgcagtctgg cgcgggggct ggcgcaggat
      541 tgacgcttct tcctggccag gggccctgct tttcgccccg tttgtgctgg tcatgattct
      601 gggctcgatg ctgccatcca tcgacttcga cgtcctcgaa tatcacctcc aaggcccgaa
      661 agagtattac caggcggggc ggatcgcatt cttgccccat aatgtctaca caaatatgcc
      721 ctttggcgtt gagatgctcc acctgatggg gatggaggtg ctgggcgatt tttggtgggg
      781 aggactggcc ggccagcttt tgatcgcgct ttttgcgccg gcggcggcgg tattgatcgc
      841 cgcgactgcg tcacgcagtg gctcggcccg cgcggcctgg gtcgctgcga ttgtctacct
      901 ttagacaccg tggatctatc gtctggcggt gatcgcctac gtcgaaggtc cgctttgttt
      961 ttatcacgcg gcgcttttgt gggcggtagt ccgcggctgg gctgatcggt cactgccgcg
     1021 tcgaaggatc tggttcgcga ttggcctttt agccggctgt gccatgggat gcaagtatcc
     1081 agcattgatc tccgctgtcg tgcctttcgg ggtcgtcgcg ctctgggatt gccggcgcag
     1141 tcgctcgctg gaaccgctgc ttttttacgt gctgggatgg ggaatcgtga tggggccatg
     1201 gctggtcaaa aacgtgattg acacggggaa ccccgtgtat cctcttgccg attccgtctt
     1261 ccacggccgc ttttgggatc aggcgctcga acagaagtgg tccgctgcac acggccgtag
     1321 gccggtctct taccaagagt taattggatc tttgatcgat gtcgccggtc gttccgattg
     1381 gcaatcgccg ctttacgtgg ccctggcgcc actggctttg ttgcggcccg gttcacgacg
     1441 gctcgccatg gcgctctggg gcctcgtggc ctacctgttt gcaacctggt ggctcttgac
     1501 tcaccggctg gaccggtttt ggctcccgct tcttccagca ctggcgatcc ttgcaggact
     1561 gggagccgac tgggtccgaa accgaggatg gaccctcgtg ctgggagtga tcatggcgat
     1621 cggacttctc gcgaatttca cctacatcac gacggccctc gctggcttga acgaatggac
     1681 gggagacctg gtctttctcc ggcgtgacat tccgaggcgc tggaatgcac cgttggccag
     1741 actcgacact gagcttcctg cgaacgcgcg tcccttactt gtcggccagg ccgcggtttt
     1801 ccatctaact cacccggttg cctacaacac cgtcttcaat cccgagacga tcgagctcct
     1861 tgccaaagac aagaacaacg acgagttcca tgactcgctt cggcagcgta agctcagcca
     1921 catttacgtc gactggaagg aaatcaagcg gcatcgccag cccggcggtt acgggttcac
     1981 cgatttcgta acaccgtcga gattcgcgca atgggtcgcg gacggcgtgc tcgagcgacc
     2041 actcctcgtg ggacccgaac aagagctata ccaggttcgc taaagtcatt tgattccatt
     2101 tgatcatact ttgctaaaac aagttaggat tcatggacga tttgacgatc gtgaccggcg
     2161 gcgcgggttt catcggtagc cacctggtgg cgcacctggt caattctggc caacaagttc
     2221 gtgtgattga gcgacccggc gtcgcggtcg accacttgcc ccgaatcgcg cagctacact
     2281 tcgccgacat tcgcgaccgc gcggcgctag agcccgcgtt ggcaggaggc aaatgggttt
     2341 atcacctggc ggcaaatccc cacctgtggg ttcgggatcg ccgcgagttc caagaggtga
     2401 atttcgaggg cacgaaaaac gtcctagacg tatcgctaaa agcgggtgcc cagcgcattc
     2461 ttcataccag cactgagagc attctcacca aagcggaggt gactggcccg attgacgaaa
     2521 acatcgaagt ctctctgagc gatgctgtcg gtccgtactg tcggtcgaag ctgctcgccg
     2581 agcggtatgc cttttctctg gcc
//
LOCUS       DATAQL010001466         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2289099, whole genome shotgun sequence.
ACCESSION   DATAQL010001466 DATAQL010000000
VERSION     DATAQL010001466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289099"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VHS97_25370"
     CDS             <1..352
                     /locus_tag="VHS97_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451609.1"
                     /translation="FSTSSDAVVRYLEDSGNHQGDDRFRRFRAAAFPDALTYACVDLD
                     AVHQLAGKHHARLLQILAARQNRPPALVERDLAQALALAKLFRAAFITSRLHADATAV
                     HRSAGLIVRDQDAK"
     gene            complement(430..1479)
                     /locus_tag="VHS97_25375"
     CDS             complement(430..1479)
                     /locus_tag="VHS97_25375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451610.1"
                     /translation="MFAHARRRAWRRPALSPRLEPLEGRELLSTLFTSPSATLPVRTT
                     GGIFQIQVSGPGVVKAHPAGRSAIQLTAFGTTSATTITIGQLRPPWHSPSRLLVLNKV
                     VVVSGQLGSFNATPSELNGSMTQINNTVNTLEFGEIGPNAQIKVNGDVGTMTANQVDL
                     GPLGQVVISGALNTNDLTGSMTIGNLTVNSGRFVIGADSIAPISVTGDMTISKNGLFS
                     VGRDMDGSLTVNGNLELDTGGQLFVGRNMSNLTVNGNVIVNPSGSGIVVNGALGSLSV
                     TGIFQGQGGTSAPTLFDLGVGLTLDGLNIAGGTLSQNGLINANIRAGSTISGVNITYG
                     TVNSTIQPNTPPPVV"
     gene            complement(1916..2083)
                     /locus_tag="VHS97_25380"
     CDS             complement(1916..2083)
                     /locus_tag="VHS97_25380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451611.1"
                     /translation="MLFRMVSQEKRPVPAGTWSIDVGLEDVTEKIYPPFTKVDREADY
                     ARTRDVFQKKR"
     gene            2398..>2756
                     /locus_tag="VHS97_25385"
     CDS             2398..>2756
                     /locus_tag="VHS97_25385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451612.1"
                     /translation="MNEDNVSDGVLSQEPSNPTVHGRRRSNARKVSLGDTSPRMTPGG
                     IESFEIKESVAAAQEAEAAAVRDIIASAPVHDIPAVANSLRAILDGASPEDAALLRGV
                     LLDNAAGTEAPLDTYPD"
BASE COUNT          518 a          850 c          808 g          580 t
ORIGIN      
        1 cttcagcacg tcgtcagacg ccgtcgtgcg ttacctggaa gattcaggaa accaccaggg
       61 tgacgaccgc ttccgccggt tccgggccgc ggcattcccc gacgccctta cctatgcgtg
      121 cgtcgatctc gatgccgtcc atcagctcgc cggcaaacat cacgctcgcc tgcttcaaat
      181 cctggccgcc cgccagaacc ggccacccgc cctggtcgaa cgtgacctcg cccaggctct
      241 ggccctggca aaactttttc gagccgcttt tatcacgagc cgattgcacg ccgacgccac
      301 tgccgtgcac cgaagcgcgg ggctaatcgt gcgcgatcaa gacgccaagt aaccggcctt
      361 tcctcggcgc ttgaccgcgc gatcggtcga cggctcgttc gcaaccgcgc gaccggtgcg
      421 ttacttcgct catactaccg gcggcggcgt gttgggctgg atcgtgctgt tgaccgtgcc
      481 gtaggtgata ttcacacccg aaatagtgct gccggcacga atattggcgt tgatcagtcc
      541 attttggctg agcgtacctc cagcaatgtt caggccgtcc aaagtcaggc cgacccccag
      601 atcaaagagg gtgggggcgc tggttccccc ttggccctgg aagattccgg tcacgctcag
      661 tgagccaagc gcaccattga ctacgatacc gctgccgctg ggattgacga ttacgttgcc
      721 gttaacggtc aggttggaca tgttgcgacc gacaaagagc tgaccgcccg tgtcgagttc
      781 gagattgcca ttgaccgtga gcgaaccgtc catatcgcgg ccgacggaaa acaatccgtt
      841 cttactgatc gtcatatcac ccgtcaccga aatcggtgcg atcgaatcgg caccgatcac
      901 aaaccggccg ctgttgaccg tcaagttgcc gatcgtcatc gagcctgtca ggtcatttgt
      961 gttgagtgca cccgagatca ccacttggcc aaggggtccg aggtcgacct gattggcggt
     1021 catcgtgccg acgtcgccgt tgactttgat ttgcgcgttt gggccgatct ccccgaactc
     1081 gagggtattg acggtgttat tgatctgggt cattgacccg ttcaattccg atggagtcgc
     1141 attgaaacta ccgagctggc ccgagacaac gaccaccttg ttcagaacca ggaggcggct
     1201 gggggaatgc caggggggtc taagctggcc gatggtgatc gtcgtggcac tggtcgtgcc
     1261 aaacgcagta agctgaatgg cggagcggcc cgccgggtgg gctttgacca cacctggacc
     1321 gctgacctgg atctgaaata ttccaccggt cgttcgcacc ggaagggtcg ccgagggact
     1381 ggtgaataat gtagagagca gctctcggcc ctccagcggc tccagtcgcg gtgagagtgc
     1441 cggccgccgc catgctcgtc gtcgcgcgtg cgcaaacatc aataaggctc cttccctttt
     1501 cccgatccgg gaatcctgca aatccggaca atcttgcgcg accggcatca tcgctacagt
     1561 cggcgctttg atcgcatggc cgtgatcact gcgactctca cgttctcctt tttcggtcgt
     1621 gagggggctt caactttgtt tggcttggca actgagttcg ttccggtggg gttgtgtgcg
     1681 cggttgaaag gtccgctcaa cagccttgtc gcgaaaatca aacgtggact gcaggtagta
     1741 gaggacccgc tcgcggacgt tctccgcgag acgcacgggg aaagttggtc tactcgagtt
     1801 ccgtcaaaac tcgaacgccg cgcctggtcg gcatggctgc catatttgat cgtcgctttc
     1861 ccgtagtcga atgaccagag cggcgtcgat ccagtcagaa cgttgttacc ttgccctaac
     1921 gttttttctg gaagacgtcg cgcgttcgcg cgtagtcggc ctcgcggtcg accttggtga
     1981 atggcggata aatcttctcg gtgacgtcct cgaggccgac atcgatcgac caggtgccgg
     2041 cgggtaccgg gcgtttttct tgcgaaacca tgcggaatag catttggccg gtttctaccg
     2101 agctccggca gaagtcgtct tgctagcacg ctcttggtgc cctctcgtgg ccaggctaag
     2161 attagagata tggttcggat ttgcagttca acagatgcgg agcacgcgac cgtaccgaat
     2221 cgcggccggg cacgcgacaa tcggtgaaat cggagtgcgg atctcttacg cgagcgtgtc
     2281 tctgccccac ttgcatggtc cgccattgaa gtgggatctc caacgccagg aagctcagct
     2341 cattctaccc ttgatcaatt tgacgcactg cctttctgcc gtgttgcagg accagtcatg
     2401 aacgaagata acgtctccga cggtgtgctc tcccaagagc cttcaaatcc gacggtccat
     2461 ggccgccgcc gttccaacgc gcgaaaggtc agcctcgggg acacctctcc ccgcatgact
     2521 ccgggaggga tcgagtcgtt cgagatcaag gagtcggtcg ccgcggcaca ggaagcagag
     2581 gccgccgccg tgcgcgacat catcgccagc gccccagtcc atgacatccc cgccgtggcc
     2641 aattccctac gagcgatcct cgacggggcc tctccggaag acgccgccct gctccgcggc
     2701 gtcctgctgg acaatgcggc cggaaccgaa gcgccgctcg acacttaccc cgatga
//
LOCUS       DATAQL010001467         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2289286, whole genome shotgun sequence.
ACCESSION   DATAQL010001467 DATAQL010000000
VERSION     DATAQL010001467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289286"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VHS97_25390"
     CDS             <1..434
                     /locus_tag="VHS97_25390"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX3451613.1"
                     /translation="LLVWAATVALSAWTPPHFAWPEAPFLRPERFLPFWSYYVSSRLT
                     DLTGLFSQLLAFVPLGVLLAVRTRRQSIAGAALIGLAGGFAVEFVQIFLPARTAELTS
                     VLMAATGTALGVALWRWGESLHHSSHGVARYRIGPQAERRV"
     gene            complement(490..774)
                     /locus_tag="VHS97_25395"
     CDS             complement(490..774)
                     /locus_tag="VHS97_25395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451614.1"
                     /translation="MDHAPCPRCGDSTTVAGWSDLPDQSHWFQPDGIHWLRHLLWHFG
                     EKTHVHLPDRFRACLGCGLIWNSLPPEQLREVLSREAVAIGDEWTPPLID"
     gene            1246..>1772
                     /locus_tag="VHS97_25400"
     CDS             1246..>1772
                     /locus_tag="VHS97_25400"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3451615.1"
                     /translation="MFCTRLSAMALIAAGATLGYFAASIDFGGSRLASAASPSDGPAI
                     LGENSDCCSEGANKSLLLASTVKAAAPAVAQERKPNIVVIMGDDVGVWNIGAYHRGMM
                     AGRTPNLDRLAAQGMLFTDYYAEASCTAGRANFITGQLPIRTGMTTVGQAGSPIGLPA
                     QAPTTATVLKSMGYAT"
BASE COUNT          310 a          551 c          544 g          367 t
ORIGIN      
        1 cccttttggt ctgggccgcg accgtcgccc tgtccgcctg gaccccgccc cacttcgcct
       61 ggcctgaagc ccctttcttg cgtcccgagc ggtttttgcc attctggtcc tattatgtaa
      121 gcagccggct cacggacctt accgggctgt tcagtcaatt gctggccttc gtgccgctgg
      181 gggtactttt ggccgtgcgc actcgccgcc aatcgatcgc gggcgctgcc cttatcggcc
      241 tggcgggtgg atttgcggtc gaattcgtcc agatcttttt gccggctcgc acggccgaac
      301 tcacctccgt gctcatggcg gccacaggca cggccctcgg agtggcactc tggagatggg
      361 gtgagtcact gcatcactcg agccacgggg tcgcgcggta tcgcatcggc ccgcaagccg
      421 agcgcagggt ttgattccca ccaccgagat gcggatcgct cttgataacg agttgtgcca
      481 ctattgagct cagtcgatga gcgggggcgt ccattcatcg ccgatggcca ccgcttcgcg
      541 gctgaggacc tcgcgcaatt gttcaggggg caggctgttc cagatcagac cgcaccccaa
      601 gcaggcccgg aatcggtctg gtaggtggac atgtgtcttc tcgccgaaat gccaaaggag
      661 gtgccgcaac caatggatgc catccggctg gaaccaatgc gattggtcag gtaaatcgct
      721 ccatccggcg accgtcgtcg agtcgccgca ccgcgggcag ggtgcgtgat ccatgttgac
      781 ctcttgcccc aagcgccaga agcgggtttg atcggcccgt cgagggagag cctcgagacg
      841 tgaggacgat ccacttttta tttgatcagc gcccgccccg aacttcaata ggtgaatgca
      901 gaaaatgtta atgcgtacca tagcacgtcg cagattcgag cttggcgtta gaaattgtgc
      961 tcccccggat tgcccgacgg ccttgatcat gtcgcaagct gggtcgaggc cggaccggac
     1021 tgtcgctcga cgcctccggt tcgacccaag ctcttcacct cgacgactgg ctcaaacgcc
     1081 gcgcggatgt caaacgacag catggctcgt cgctgtcggg ctgggaacta tagcgggctg
     1141 tcaatttcgt ggatattatc tcggacggtc agggtcattg attcagactt tttttgatca
     1201 attcgcccga atgtttcgcg ctaaccacag aggagtcttc cccgcatgtt ttgcacccga
     1261 ctctccgcga tggcgctcat cgccgccggc gcgacgctag gttatttcgc tgcctcgatc
     1321 gactttgggg gttctcgctt ggcgtccgcg gcgagcccgt cggacggccc ggcgatcttg
     1381 ggggagaata gcgactgttg ttcggaagga gcgaacaaga gtttgttgtt ggccagcacg
     1441 gtaaaggcag cggccccggc ggtcgcccaa gagcgcaagc caaatatcgt cgtcatcatg
     1501 ggtgacgacg tcggcgtctg gaacatcgga gcgtaccacc ggggcatgat ggccgggagg
     1561 acgcctaacc tcgaccggct cgccgcgcag gggatgctgt tcaccgacta ctacgccgag
     1621 gcgagctgca cagccgggcg tgccaatttc atcaccggtc agctccccat ccgcacgggc
     1681 atgacgaccg tcggccaggc gggatcgccg atcggtctcc ccgcgcaggc accgaccacc
     1741 gcgacagttt tgaagtcgat gggctacgca ac
//
LOCUS       DATAQL010001468         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4120561, whole genome shotgun sequence.
ACCESSION   DATAQL010001468 DATAQL010000000
VERSION     DATAQL010001468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4120561"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1133
                     /locus_tag="VHS97_25405"
     CDS             63..1133
                     /locus_tag="VHS97_25405"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3451616.1"
                     /translation="MDGQHIKVEGGKRFERRWGMRPYSRDLRERVATAVDHGEGSLRE
                     IARRFRVSLSFVARLLRRRRQTGSLDPKPHGGGHPPALDPAGLERLRELVRQQPDATL
                     DELRQRIGAPCSRMALWRAVRKLKLTRKKKSLHARERDTPENQAKRVAFLEKLAGIDP
                     HRLVFVDETGATTAMARAYGRAPRGERVVGTVPGRWTSVTLISALRLSGVVAPLAFEG
                     ATDTAAFQTYAEEALAPQLRPGDVVIWDNLKPHKDAAVARAIERAGARVESLPPYGSD
                     LTPIEELWSKAKEFLRSVAARTTDAVYDAMGAALRSVRPEDIVGWFRFCGWCGEQGSK
                     TSGPGYSVDGGIHSRCSPCATQ"
     gene            1278..>1782
                     /locus_tag="VHS97_25410"
     CDS             1278..>1782
                     /locus_tag="VHS97_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451617.1"
                     /translation="MLKIPFATWIANAAVGLTGIYGDVTRQAEVADCSRQTIYDHAQK
                     VQSAVEDAHDGGPTRAALIEQNHLLQRENSQLWDGLALTVEFPLSQQREFAVTACAMG
                     LSLNQVLVLLALILGTQAGPGRSTMHRWIKAAALAAGRVLKSLDERCRALVLVGCLDE
                     IFFHGRPV"
BASE COUNT          300 a          585 c          609 g          288 t
ORIGIN      
        1 tgacttacgc tcgacgaccg ggcagaaaac ccaaagaaac agcttgctag cgcaattccc
       61 ccttggatgg ccaacacatt aaagtggagg gggggaagag atttgagagg aggtggggga
      121 tgaggccgta ttcgcgagat ctccgcgagc gcgtcgccac ggcggtcgat cacggcgaag
      181 gctcgctccg ggagatcgcc cgccggttcc gcgtcagcct ctcgttcgtc gcccggctgc
      241 tccgccgccg acgccagacc ggctcgctcg atcccaagcc ccacggcggt gggcacccgc
      301 ccgccctcga ccccgccggc ctggaacgcc tccgcgagtt ggtgcggcag cagcccgatg
      361 ccaccctcga cgagctgcgc cagcggatcg gcgccccctg cagtcgcatg gcgctgtggc
      421 gcgccgtgcg gaagttgaag ctcacccgca agaagaagtc gctccacgcc cgggagcggg
      481 ataccccgga gaaccaggcc aagagggtgg cgttcctgga gaagttggcc gggatcgacc
      541 cgcaccggct ggtgttcgtg gacgagaccg gggcgaccac ggcgatggcg cgggcctacg
      601 gccgtgctcc ccggggggaa cgcgtcgtgg ggacggtgcc cgggcggtgg acctcggtga
      661 cgctgatctc cgccctgcgg ctgtcggggg tggtggcgcc tttggcgttc gagggggcca
      721 ccgacacggc cgccttccag acctacgccg aagaggcgct ggcgccgcag ttgcggccgg
      781 gcgatgtggt gatctgggac aacctcaagc cgcacaagga tgcggccgtg gcgcgggcga
      841 tcgagcgggc cggggcgcgg gtcgagtcgt tgccgccgta cggttcggac ctgacgccga
      901 tcgaggagtt gtggtcgaag gcgaaggagt tcctgaggtc ggtcgcggcg cggaccaccg
      961 acgcggtgta cgacgcgatg ggggcggcgt tacggtctgt tcgtcccgag gacatcgtgg
     1021 gctggttccg cttctgcggc tggtgcggtg agcagggatc gaagacttct gggcctgggt
     1081 actccgtcga cggaggaatc cactcccgct gtagtccgtg cgcaactcaa tagtgggcac
     1141 cgtcccaaac tgacctcaat tggccgcatt caggttgaca attcacaact ccaggccatg
     1201 acatcttgat cgtgcaaggg ggtggatccc cagagtggga ttgcttacgt gatccacact
     1261 ccaaaaaggc ccaccccatg ctcaagatac ccttcgccac ctggatcgcc aacgccgccg
     1321 tgggactgac aggcatctat ggcgatgtca ctcgccaagc cgaggtcgcc gactgtagcc
     1381 gacagaccat ctacgatcat gcccagaagg tccaatccgc cgtcgaggac gcccacgacg
     1441 ggggaccgac ccgggccgcc ttgatcgagc agaatcacct cctccagcgg gagaattccc
     1501 aactctggga cgggctcgct ctgaccgtgg agttcccctt gagccagcag cgcgagttcg
     1561 ccgtcaccgc ctgtgcgatg ggattgagcc tcaaccaggt cctcgtcctg ctcgccctga
     1621 tcctgggcac gcaggcaggc cccggacgtt ccacgatgca ccgctggatc aaagccgcgg
     1681 ccctggccgc gggacgtgtc ctgaagtccc tcgatgagcg atgccgggcg ttggtcctgg
     1741 tcggctgcct cgatgagatc ttcttccacg gccgccccgt gc
//
LOCUS       DATAQL010001469        12348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5190525, whole genome shotgun sequence.
ACCESSION   DATAQL010001469 DATAQL010000000
VERSION     DATAQL010001469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12348
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5190525"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1172)
                     /locus_tag="VHS97_25415"
     CDS             complement(219..1172)
                     /locus_tag="VHS97_25415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451618.1"
                     /translation="MPDPIELLKAMSMAAAVAAAMLGIFGWPRRKEQPTWIDDGWVLG
                     VTAGFILGCWAIGIRPHWPPREDLDRLLALVIPAVLAVELVAAFPKVPRWLIWPLRMV
                     IVAWGTRVLLHGSGYITDLAGPATSEWSPAQTWTILSGVAAIEAAVWVCLALLARRAP
                     GSSVPLSLAIVIAGSAVTVMLSGYSTGGQVGLPLAAALLGSTVAAAVLPRSSPKTAQL
                     GVPIVGLFSLLVIGRFFGQLTTAHAILLVLAPLLAWIPELIYPRRLPPWARGLLRLVL
                     VGSLVAAVLVHAQSKFNHDFQSPSGSGSKEPSLEDYMNFGK"
     gene            complement(1198..1773)
                     /locus_tag="VHS97_25420"
     CDS             complement(1198..1773)
                     /locus_tag="VHS97_25420"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX3451619.1"
                     /translation="MHKQFLQMALAGALSFGLGTVVKADDYAIDAVHSGVSFQISHVG
                     LAYIHGRFDDFSGNFTIDTSDPTKSAFSLSIKSESVDTNNSKRDDHLRSPDFFNVKQF
                     PTISFTSTSVKPIDGGYEVTGDLTIHGATKPVTFSLKGGASAEFPKGVSRTGFSTSQI
                     VVKRSDFGVGKPMPVLGDEVYVSISFEGTKK"
     gene            complement(1846..2331)
                     /locus_tag="VHS97_25425"
     CDS             complement(1846..2331)
                     /locus_tag="VHS97_25425"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3451620.1"
                     /translation="MMRTTGSGGRERRRLPILLNPEELELRTWTQMMRTSSRMERRTE
                     QALERHGLSIAQFDILATLGFEQGITQQDLAERLLVTKGNICGMIDRMEVSGWVERRP
                     DPGDRRVNRLFLTRHGKTKLGQTLPEQHALLKKLMSALEPAEIQSLYQFLDRLDEAVD
                     E"
     gene            complement(2401..4026)
                     /locus_tag="VHS97_25430"
     CDS             complement(2401..4026)
                     /locus_tag="VHS97_25430"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3451621.1"
                     /translation="MMSIRSAFLVITIVFLPASGFAQAPAKAQERAPAQTGGGPSPSA
                     TEQSGEVKAVTQAILEEIDKRSELMANIEYLCDMIGPRLTGSPNLTKANHWTRDKFQQ
                     YGLSNAHVEPWTIERAWTRGEAAGRVVVPVEQRILLESAGWSPSTKGPKRGPIIHLKA
                     QSVDELSPYKGNLKGAWVLFSEVSTQPSPKQPAPNLDGEMRRRMRDYTRMREFRPALK
                     KFLVEEGAAGVLRDSNKEHGLINMTGASDNFTPAELPEAFLTTESYGLIWRLLKRGPV
                     EIELDLKNSFSTGEVQVFNTVAELKGGDKADEVVLIGGHIDSWDLGTGATDNGTGIMA
                     VLEAARALKVVDAKPKRTIRFVLFSGEEEGLHGSRAYVKAHEKEMPKIAGVLIHDMGT
                     GRVKSIGLQGRYDLREAMDRVVEPFKEALNLEELSMRSMMGTDHLSFLPHGVPAFAVV
                     QDGAEYRKTHHTESDTVDKVYPDEVNQGAKVLAAWAYNVAMLPEILPRDPKPPQRGMF
                     DSPEFRQPRDEKNKSEDSKQPDAKPRVAAEAGR"
     gene            complement(4215..5834)
                     /locus_tag="VHS97_25435"
     CDS             complement(4215..5834)
                     /locus_tag="VHS97_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX3451622.1"
                     /translation="MSSATPATAAISGTGVLPPVLGRLLSGTFWLALRVPLQFVFALL
                     TTRLVLDAIGPFETGAYAFAWGFGFFQFLFEFGASSALQRQISDAWTRGDRAGVDRAI
                     ACGMTFYAAMAVLQVAALLGVAYWALPHSKLHVSSYPLVIKLLWLQAVTAPCFGMSVV
                     VSSVLQAARRYAFVPKLEVGITILRFTVLLIGVRSEVDFFRIIAAQTAIQVGLSLGPA
                     LWVMVHELGHVPHFRGARWIDYKVLGQFSFYIALIQISVVMADKLDTTILGFILAADP
                     EPAITVYTVVSKPFLQLRQTGWMLAYMVMPAVASLAAARDLKGLDRVKYDGTRLHVGA
                     LLPVGLLAWIYASPFLSLWLGDRQWDAMDALGYDLADVTWLMRLFLIAAIPIVLSVPV
                     QMSIGLNNVKVIGLSALAGSLFNLPISCFLTARLGVAGVIWGTILTTLFSNFLVPGIY
                     VFRVLEIDPRTYLRRTLIAPLAGGASLIVMTWVLRQLMPNANAGPAPWSRALSLVLHL
                     SVGTIAYLAGYLLVPGGRADFWDLFTRLRRR"
     gene            complement(5988..6533)
                     /locus_tag="VHS97_25440"
     CDS             complement(5988..6533)
                     /locus_tag="VHS97_25440"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX3451623.1"
                     /translation="MSPRQFRKAIRAGVFGERRVELLGGIPFIMSENPPHIFASSRVF
                     SALIALAARPRWFVNKDHRLELGQWLPLPDAVVLDGPDTIYGTRLARPDDVALLVEIA
                     DTSYSKDSGPKLRRYASFRIPVYWIVDLNRRLVEVHSQPFGKGKQAGYARCDTYFEND
                     FVPVVLAGNEVGRIAVADLLP"
     gene            6861..8375
                     /gene="guaB"
                     /locus_tag="VHS97_25445"
     CDS             6861..8375
                     /gene="guaB"
                     /locus_tag="VHS97_25445"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX3451624.1"
                     /translation="MHDRIAYQGITFDDVLLEPGYSDLLPHQVDTRSELTARIGLNIP
                     ILSSPMDTVTEAELAIALAQEGGLGVIHKNMSIEEQTREVDKVKRSENGIIVDPITLP
                     PDALVGEARKIMRGHNISGVPITVGGYLRGILTRRDLRFLESNDLRIEEVMTKSNLVT
                     APADTSLEEADRILTKNKVEKLLLVDDEYRLKGLITIKDIDKLDRYPHACKDARGRLR
                     VGAAVGVLDYERISSLLNAGVDVLIVDSAHGHSKNVTETVRRIKNSFDIQVVAGNVAT
                     GEGTRALIEAGADAVKVGIGPGSICTTRVVSGVGVPQVTAIYQSAKAAAGKVPIIADG
                     GIRYSGDITKAIAAGAHVVMIGGLFAGLAESPGDTIIYRGRSFKTYRGMGSIGAMAKG
                     SHERYRQEPGTRIQDKDDKTTPNQKLVPEGVEGRVPYKGPLADFVFQLVGGLRAGMGY
                     CGARTIDDLRTKTRFIQVTSASVQESHPHDIVITQEAPNYSSFSNENELGRGSV"
     gene            8428..10980
                     /locus_tag="VHS97_25450"
     CDS             8428..10980
                     /locus_tag="VHS97_25450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451625.1"
                     /translation="MRPTTSVLTTAVLGAAVTASTVLAQQLRAPDGAPQGDSAAAGST
                     PATGSASPVDSSGLFGMAPSRGARHLLRNGLDYLNYQQYERALKFLRDAETRQKELNE
                     PEKLVLKQGIERAQRGLREAADAESPYAISERSRRRNGFAPAQAETRVTANAAQTKFP
                     VQKTKTNRPTPLAPLASDGDDQGEPIRLASGETPVTNPTVDANSDTPPSQTTNAAPPV
                     GSERGQPANMPEIPQIPPVAPLADPTETGGSAHQLAAGAISPAGTGSTGAQAAATSRQ
                     TPVPPAEKQHGQPELLSSLPPLAPASELTAQATNPSPASAAPTAEPATAEVPGVTTQP
                     STPTTAQSVDTSPSPEAIKRAPVIDLETITSGATSPTASPSVDPSAPPHRESEEATPG
                     TAPGAVENTPANPPTTNVTATPTATEGVSEPPLLPPNLGRLVATPATPETSASPSATA
                     SAPLTASSGAEDLPPLPADLGRSDAANPATTASPARGNDIPASPLPAAAEASTQPAQV
                     GPAPLADAPAPIGDVQQPAGSTGDNRKPEDTGAAILAPATPLAAGADARVAPERTSAP
                     TDTETPQPPATASNSPASNVAAPTQPYPTRNPNLERQAAPTGGTSAESTPDVSSLPLA
                     LPGRMQASSSDSYIPDRAIPSSSLTPDLQREVEQIARSQVEEMHRQAQAQPPPAPAPT
                     APPDTTSSDLRTQTQLDISRAPSPAEARPIKAIPVPEDWVPLPPRNWSPQRKYWAAAA
                     TCHLPLYFQDPVLERYGHSVEQFVGPFGRFLTYPVDDPTQSTQRNQMLQPWVSAGLMG
                     LQIIAWPYNLIMDPPWEAQYDLGYYRPGDNIPTDTYWLPLHGYGPPLRGSNY"
     gene            11413..12183
                     /gene="phoU"
                     /locus_tag="VHS97_25455"
     CDS             11413..12183
                     /gene="phoU"
                     /locus_tag="VHS97_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX3451626.1"
                     /translation="MPQQRNALDSDWDNERVTVGRNFLRDMEALWSEVLKLAAVVEDA
                     LNQSVQGLCDGRVDLAHELKGRKHAVDRWEVQIERECVRVLALHQPVASDLRRVAAVL
                     KINGDLERLCDLARHIAKRVKKLAADPQAFPIPQTLENLALEALSQIHASLDALTQSN
                     VSRAQAVIAADQAVDRNYRAVQKQLKAEIIRDPDRIDTWLRLVNTARNLERIADHAAK
                     IAESVIYLKEGEIFRHRTSGSSRDASLVSLPPAASSQS"
BASE COUNT         2719 a         3886 c         3511 g         2232 t
ORIGIN      
        1 gagcttggct ttctcggact gtatctcatc tgccaccagg agaaccggcg aagccgctgc
       61 agcagcaaac atcaaccaac acaagctgcg gcgcacagaa atccgatccg tcccaaaagc
      121 ccacatggca tgctccagtt ggccgaggtt tgagtattat ttaacatcgg ccaacggact
      181 gctcttgtca agaacttcgt ttgactgtcc ctcgggcttt actttccgaa gttcatgtag
      241 tcttcaagcg acggttcctt cgagcctgat cctgatgggg attgaaaatc gtgattaaac
      301 ttgctttgcg catgaaccag gacggccgcg accagcgatc cgaccaggac cagtcgcaat
      361 aggccgcgcg cccaaggagg gagccggcgc ggataaatta actcgggtat ccaggcgagc
      421 aagggggcaa gaacaagcag aattgcatgc gccgtcgtga gttgaccaaa gaaacggccg
      481 atcacgagca ggctgaaaag cccaacgatt ggcactccga gttgtgcggt cttaggtgat
      541 gatcgaggca agacggccgc ggcgactgtc gaccctagaa gtgcagccgc gagcggtagc
      601 ccgacctgtc cgcccgtgga ataacccgag agcatcaccg taacggccga gccagcaatc
      661 acgatcgcca aggataacgg cacggacgac ccgggcgctc gacgagccag aagggccaga
      721 caaacccata ccgctgcttc gatcgccgcc acgcccgaca aaatcgtcca cgtctgcgcc
      781 ggtgaccatt cgctcgtcgc cggcccagca aggtcggtga tatagcccga cccatgtaat
      841 aagactcgag tgccccaggc cacgataacc attcgtaatg gccagatcag ccagcgtggc
      901 accttgggga aagccgcgac caactcgacc gcgagcacgg ctggaatcac cagggccagc
      961 agacggtcca gatcttcgcg cggaggccaa tgaggccgga ttcctatggc ccagcacccg
     1021 agaataaagc cggccgtcac acccaacacc cacccgtcgt cgatccaggt gggttgttcc
     1081 ttgcgcctgg gccagccaaa gattccgagc atggcggccg caacggcggc cgccatgctc
     1141 atcgccttca agagttcaat cggatcaggc atgagctcga tcctctttta cgcatactta
     1201 ctttttcgtg ccttcaaagc tgatcgagac atagacctcg tcgcccagaa cgggcatcgg
     1261 cttgcccacc ccgaaatcgg atcgcttgac cacgatctgg gatgtcgaaa agccggtccg
     1321 actgactccc ttggggaact cggccgaagc gcctcctttg agcgaaaaag taacgggctt
     1381 ggtggcaccg tgaatggtca ggtcgcccgt gacttcgtag cccccatcga tcggcttgac
     1441 cgacgtgctg gtaaagctga tggtcggaaa ctgcttgacg ttgaagaagt cagggctacg
     1501 gaggtggtca tcacgcttcg agttattggt atccacgctc tccgacttga ttgagagcga
     1561 gaacgccgac ttggtggggt cgctcgtatc gatcgtgaaa ttgccgctga agtcgtcgaa
     1621 ccggccatgt atgtaggcta aaccaacgtg cgaaatctga aaagaaacgc ccgagtgcac
     1681 ggcgtcgatg gcgtaatcgt cggccttgac gactgttccg aggccaaaag agagcgcacc
     1741 cgcgagggcc atctgaagaa actgcttgtg catcagtcta gtcctttctg atcgaaaaaa
     1801 tggaaacccc gggttcaaac cgaaacgtga agtcatgcct tgatctcatt cgtccacggc
     1861 ttcgtcgagt cgatcgagga actgataaag ggactgaatt tccgcgggct ctaatgcact
     1921 catcaacttc ttgaggagcg catgctgctc gggaagggtc tggccgagct tcgtcttacc
     1981 atgacgggtc aggaataatc gattcacgcg gcgatccccg gggtcgggcc tgcgctcgac
     2041 ccatccactc acttccatcc ggtcgatcat cccgcagata ttccccttcg tgaccagtag
     2101 ccgctcggcg agatcctgct gcgtgatgcc ctgctcaaac cccagcgtcg ccaggatgtc
     2161 gaactgagcg atcgacaggc cgtggcgctc gagcgcctgc tccgttcgcc gctccatacg
     2221 gctcgaggtg cgcatcatct gagtccaggt gcgcaactcc agttcttcgg gattgagcag
     2281 tatcggcagc ctccgtcgtt ctcggccgcc gctgcccgtc gttctcatca tgtcgtttac
     2341 ttgtaaacta tctcggtgaa gcggtcaaga gtagaggccc tcgtggtgaa ccttacgaag
     2401 tcatcggccc gcctcggcgg cgacgcgagg cttggcatcg ggttgcttcg agtcttccga
     2461 tttgttcttc tcgtcacgcg gctggcggaa ttcgggactg tcgaacatcc cgcgctgggg
     2521 tggcttgggg tcgcgcggca ggatttccgg cagcatggcc acgttgtagg cccacgctgc
     2581 cagcaccttc gccccttggt tgacttcgtc gggatatacc ttgtcgacgg tgtcgctctc
     2641 ggtgtgatgc gtcttgcgat actcggcccc gtcttgaacc acagcgaagg caggcacgcc
     2701 atgaggaagg aacgacaggt ggtcggtccc catcatgctg cgcatgctca gctcttcgag
     2761 gttgagcgcc tccttgaacg gctcgacgac gcggtccatc gcctcgcgca gatcgtagcg
     2821 accttgcagc ccgatgctct tgacccgccc ggtacccatg tcatggatca agacgccggc
     2881 gatcttgggc atctcctttt catgtgcctt cacataagca cgcgatccat gcagcccttc
     2941 ctcttctccg gagaagagga caaagcggat ggttcgtttg ggctttgcgt ctacaacttt
     3001 aagggcccgc gcggcttcga gcaccgccat gatgccggtc ccgttgtcgg tggcgcctgt
     3061 tcctaagtcc cagctatcga tgtggccgcc gatcaagacg acctcgtcgg ccttgtcgcc
     3121 cccctttagc tccgcgacag tattaaaaac ctggacctcg ccggtgctga acgagttttt
     3181 cagatcaagc tcgatctcga cggggccgcg cttgagcaac ctccagataa ggccatacga
     3241 ctcagttgtc agaaacgctt cgggcaattc cgccggcgtg aagttgtctg atgcgccggt
     3301 catgttgatc aagccgtgct ccttgttcga atcacggagc acgccggcgg ctccttcctc
     3361 aaccagaaac tttttcaggg ccgggcggaa ctcgcgcatc cgcgtatagt cgcgcatccg
     3421 ccgccgcatc tcgccatcca gattgggcgc cggctgcttg ggcgagggct gcgttgagac
     3481 ctcggaaaac aagacccagg cccccttgag gtttccttta taggggctca gctcgtcgac
     3541 ggactgcgcc ttcaaatgga taatcggccc gcgcttcggg cctttggtcg atggactcca
     3601 acccgccgat tcgaggagga ttcgctgctc aaccggcacg accacacgac cggcggcctc
     3661 tccacgggtc cacgcgcgct cgatcgtcca cggctcgacg tgggcgttcg acaacccata
     3721 ttgctggaac ttatcgcggg tccagtggtt ggccttggtc aagttgggcg agccggttaa
     3781 ccgcggcccg atcatgtcac aaaggtactc gatattggcc atcagctctg aacgcttgtc
     3841 aatttcctcc aggatcgcct gggtcaccgc cttgacctcg ccgctttgct cggttgccga
     3901 cgggcttggg ccgccgccgg tctgggctgg tgctctttcc tgggccttcg ctggcgcctg
     3961 ggcgaaacca gacgcaggta gaaatacgat cgtgatcaca aggaacgcgc ttcggatcga
     4021 catcatgatg aaaactatcc cccctgacgg ttgagtcgat ttggaccggc cttgcctgag
     4081 aataaacgga gagggcgtgt acgatcggtg atttccaggt aaagagtatg ccccgcgcgc
     4141 aagcgcgcgt ccatcgtgca gtagcagcgc ctccggtgac caaatggctg gctgaacaag
     4201 ccgaccaagc tctctcaccg tcttcggagc ctggtaaaca gatcccagaa gtcggctcgc
     4261 ccgccgggca caagcaaata gccggccaag taggcaatcg tgcccacgct caggtgcaat
     4321 accagcgaga gggcgcgcga ccaaggagcc ggtccagcat tcgcgttagg catcaactgt
     4381 cgcaaaaccc aggtcatcac gatcagggag gctccccccg ccaagggagc gatcaaggtc
     4441 cgcctcaagt acgtccgcgg atcgatctcc agtacgcgga atacataaat tcccggcacc
     4501 agaaaattcg agaacagggt cgtcagaatc gtaccccaga tgactcccgc cacgcccagc
     4561 ctggcggtca gaaaacaact gatcggcagg ttgaagagcg agccggccag cgccgacaaa
     4621 ccaatgacct tgacgttgtt caagccgatc gacatctgaa cggggaccga aagcacgatt
     4681 ggtatcgcgg cgatcaagaa gagccgcatc agccatgtga cgtccgcaag gtcataaccc
     4741 aaggcatcca tcgcatccca ctggcggtcg ccaagccaaa gagacaggaa cgggctggca
     4801 tagatccacg ccaagagccc taccggcaag agcgccccga cgtgcagccg ggtaccgtcg
     4861 tacttgaccc gatccagccc ctttagatcg cgcgcagctg ccaggctggc gacggctggc
     4921 atcaccatgt aagccagcat ccagccggtc tggcggagct gcaagaatgg cttgctgacc
     4981 acggtgtaaa cggtgatggc tggctcggga tcggcagcaa gaatgaagcc cagtatcgtc
     5041 gtgtcgagct tgtcggccat cacgacgctg atctggatga gcgcgatata aaagctgaac
     5101 tggcccagaa ccttgtagtc gatccatcgt gcgcctcgaa aatgcggcac gtgtcccagt
     5161 tcatggacca tgacccagag cgccggcccc aggctcagac caacctggat cgcggtctga
     5221 gccgcaataa tccggaagaa gtcgacttcc gagcgtacac cgatcaagag cacggtgaat
     5281 ctcaggatgg tgattccaac ctcgagcttg ggaacaaaag cgtagcggcg cgccgcctgg
     5341 agcacgctcg agaccacgac cgacataccg aagcatggag ccgtcaccgc ctggagccag
     5401 agaagcttga taaccagcgg atacgagctg acatgaagct tgctgtgggg caatgcccag
     5461 tatgcaactc ccagcaatgc cgcaacctgc aaaacagcca ttgccgcata gaatgtcatt
     5521 ccgcatgcga tcgctcggtc aacccccgcc cggtcgccgc gcgtccaggc atcggagatc
     5581 tgccgctgta gggccgagct cgcgccgaac tcgaagagaa attggaaaaa accaaagccc
     5641 catgcgaacg cgtaggcccc tgtctcgaag ggcccgatcg catctaagac caagcgagtg
     5701 gtcaggagcg caaagacgaa ctgcaacgga actcgtagcg ctagccaaaa tgtcccgctc
     5761 aacaggcggc ccaaaacagg cggcaagacc ccagtgccgg aaatagcagc ggttgcaggc
     5821 gtggccgaac tcacggaatc cttcctgggc gaggtcaatc cattcgctcc ctggcagcga
     5881 aagcgagatc atagctccat cttaggccta gtcgaaaggc caagatcaga gcccaggatg
     5941 aacaaatgag agtcaccgcg ccatacggca agttcgcaag tcctggttca aggcagcaag
     6001 tcggccacgg cgatccggcc gacttcattc ccagcgagca ctacggggac aaagtcgttt
     6061 tcgaaatagg tatcgcatcg agcatatccc gcttgcttgc ccttcccgaa cggctggcta
     6121 tggacttcca caagccggcg gtttaaatca acgatccaat agacggggat tcggaaactg
     6181 gcatagcgcc gtaacttcgg accgctgtcc ttggagtacg aggtatcggc gatctcgacc
     6241 aagagggcga catcatcagg gcgggccaag cgcgtgccgt agatcgtgtc aggaccgtcg
     6301 agtaccacag catccgggag cggcagccac tgacctaatt cgaggcgatg atctttgttg
     6361 acaaaccatc tcggtcgagc cgccagggca atcagggcag aaaaaaccct ggacgacgcg
     6421 aatatgtgcg gtggattctc agacatgata aaggggatcc ctcccagaag ctcaacacgc
     6481 cgctcgccga aaacaccggc cctgatcgcc ttgcggaact ggcgcgggct catgcgatag
     6541 attccaggga tcatcgtggt atttgctccc gaaccgcttt cattcaagcg agttcttctt
     6601 gaggcacttc tgattcagca taatctaaga tctcgaaccg ggccaacgga aagacgtgac
     6661 tgggattgca tgcagtgccg gttgcccttg tcagcgaacg ccgcggcggg tttaatgtgt
     6721 agggaaaggc ccctgaatct aaccacgccc gattgatctc gcaccccgga cctttcgatc
     6781 ctgtctgccc tcgttgtcag tcagatcgat cgtcacgtcg tgccggcatc tctttccggg
     6841 ttgaagtgga gcttttcccg atgcacgacc gcatcgccta tcaaggcatc actttcgacg
     6901 acgttctgct cgaaccgggt tattccgatc tgcttcctca ccaggtcgac actcggtcgg
     6961 aattgaccgc gcggatcggc ctgaacatcc ccatcctctc gtcaccgatg gacacggtca
     7021 ccgaggccga actggccatt gcgctggcgc aggagggtgg cctcggcgtg atccacaaga
     7081 acatgtcgat cgaggagcaa acccgcgagg ttgacaaggt caagcgttcc gagaacggta
     7141 tcatcgtcga tccgatcacc ctgccgccgg atgccctcgt cggcgaggcc cgcaaaatca
     7201 tgcgcgggca caatatctcc ggagtaccga tcaccgtcgg cggctatttg aggggcatcc
     7261 tcacgcgccg cgacctccgc tttttggaat ccaatgacct gcggatcgag gaggtcatga
     7321 ccaaaagcaa tctcgtcacc gcgccggcgg atacgagctt ggaggaagct gatcggattt
     7381 taacgaaaaa taaggtcgag aaacttttgc tggttgacga tgaatatcga ctgaaagggc
     7441 tgattacgat caaggacatc gacaagctgg accgctatcc ccatgcatgc aaggacgcaa
     7501 gaggtcggtt acgagtcggt gccgcggtcg gcgtgctcga ttatgagagg atcagctcgc
     7561 tcttgaacgc gggcgtcgat gtgctgattg tcgactcggc acatggtcat agcaagaatg
     7621 tgacggagac tgtacggcgg atcaagaaca gcttcgacat ccaggttgtg gccggcaacg
     7681 tggccaccgg cgagggcaca cgggctctga tcgaggcagg cgcggatgcg gtcaaggtgg
     7741 gtatcgggcc agggtcgatc tgtacgacgc gggtggtttc gggcgtagga gtccctcaag
     7801 tgaccgcgat ctaccagtcg gccaaagcgg ccgccggcaa ggtcccgatc attgccgatg
     7861 gcggaatccg ctactccggc gatatcacca aggccatcgc cgccggcgcc cacgttgtca
     7921 tgatcggcgg cctattcgcc ggcctggccg aaagccctgg tgacaccatc atttaccgcg
     7981 ggcgcagctt caagacttat cgcggcatgg gctcgatagg cgccatggcc aagggctctc
     8041 atgaacgcta tcggcaggag cccggcacga gaattcaaga caaagacgac aaaacgactc
     8101 cgaatcagaa gctggtcccg gaaggagtcg aggggcgggt tccatacaag ggtcctctgg
     8161 ctgatttcgt cttccagttg gtcggcggct tgcgggccgg tatgggttat tgcggagccc
     8221 ggacaatcga cgacctacgc accaagactc ggtttattca agtcacatcc gcctcggtac
     8281 aggaaagcca cccccacgac atcgttatta ctcaggaagc cccgaattac tcgagcttca
     8341 gtaacgaaaa cgaattaggt cgcggatcag tttgagtgtc aaaaaaaaac tccctggttg
     8401 tcacgacaga aagcaaggag gcgtggcttg cgtcctacca catcggtatt gaccacggcg
     8461 gttcttgggg ccgccgtgac ggcctcgacc gtgttggccc aacagttgcg cgctcccgac
     8521 ggtgcaccgc agggggattc ggccgcggcc ggatcgacac cagcgacagg gtcagcctcg
     8581 ccggtcgaca gctccggtct ctttggaatg gccccgagcc gaggcgcccg gcatctgcta
     8641 cgcaacggtc tggattacct gaactaccag caatatgagc gggctctcaa gtttctccgc
     8701 gatgctgaga ctcgccagaa agaactcaac gaacccgaaa agcttgtgct caagcaaggc
     8761 atcgagcgcg cccagcgcgg cctgcgcgaa gcagccgacg cggaatcccc gtacgccatc
     8821 agcgaacggt cgcgccggcg caacgggttc gccccggcac aggcggaaac gcgagtcacc
     8881 gccaatgccg cccagaccaa gtttccggtt caaaagacca agacgaatcg acccaccccc
     8941 ctagccccgc ttgctagcga tggcgatgac cagggcgagc cgatccgtct cgcgagcggc
     9001 gaaacaccgg tcaccaaccc cacggtagac gccaattctg acacgccccc ctcccagacg
     9061 accaatgcgg ccccgccagt tggctccgag cgcggccagc cggcgaacat gcctgaaatt
     9121 ccccaaattc cgccagtcgc cccacttgct gatccgacag aaaccggtgg gtcagcgcac
     9181 cagttggcgg ccggtgcgat tagcccggcc ggaaccggat ccaccggggc gcaagcggcc
     9241 gcaacgtctc gacaaacgcc agttccgccg gctgaaaagc agcatgggca gccggaactg
     9301 ctttcctcac tgccaccgtt ggcacctgca tccgagctca cggcacaagc gacgaatccc
     9361 tctccggcca gcgctgcccc gaccgccgag ccagccacgg ccgaagtccc gggggtgact
     9421 acacagccat caacgccaac aaccgcgcag tccgtcgaca cgagtccatc gcccgaagca
     9481 ataaagagag ccccggtgat cgacctggag acgatcacgt cgggggcgac gtctccgacg
     9541 gcttccccat cggtcgatcc aagcgcaccg cctcacaggg aaagcgaaga ggcaacgcct
     9601 ggtacggcgc ctggcgccgt ggaaaatacg ccggcaaacc cacctacaac gaacgtgacc
     9661 gctacaccaa cagccactga gggcgtcagc gaaccgccac tactgccccc gaacctgggt
     9721 cgtctggtag ccaccccggc tacgccggaa acttcggcta gcccatcagc cacggcgtct
     9781 gctccgctaa ctgcgtcatc cggcgcggag gaccttcctc cgctgccagc ggaccttggt
     9841 cgttccgacg cggcgaatcc agcgacaact gcatcgccag cacgagggaa tgatatcccc
     9901 gcatccccac tccctgccgc tgcggaagcg agcacccagc cagcacaggt aggtccggcg
     9961 cccctcgcgg atgcccccgc gcccatcggc gatgtgcaac aacccgcggg atcaacgggt
    10021 gacaaccgca aaccagagga caccggagcc gcaatcctgg ctccagccac gccgctggct
    10081 gcgggcgctg acgctcgcgt cgcaccggag cgcacctccg cgccaactga taccgaaacg
    10141 ccgcaaccac ctgccacggc gagcaactca ccagcgagca atgtagcggc acctacccag
    10201 ccatatccca ccagaaaccc gaacctggaa aggcaagcgg cgcctacggg gggaaccagt
    10261 gccgaatcca cacccgacgt gtcttcattg ccgctggcgc tacccggccg gatgcaggcg
    10321 tcgagctcag attcgtacat ccccgaccgc gctattccat cctcaagcct aacccctgac
    10381 ctacagcggg aggtcgagca aatcgcccgc tctcaggtag aggaaatgca tcggcaggcc
    10441 caggcccagc cgccgccagc gcctgcgcct accgcacctc ccgacacgac gtcgtcggat
    10501 ctacgcacgc aaacccagct ggacatcagc cgcgccccca gccccgccga agcgaggcca
    10561 atcaaagcca tcccggttcc cgaggactgg gtccccttgc caccccgtaa ctggtcacct
    10621 cagcgcaagt actgggcggc cgccgcaacc tgccatttgc cgctttactt ccaggatcca
    10681 gttctggaac gctacggcca cagcgtcgaa cagtttgttg gaccgttcgg acggtttttg
    10741 acctatccag tcgacgaccc cacacagtca acccagcgca atcagatgct ccagccgtgg
    10801 gtctcggccg gcttgatggg cctacagatc attgcctggc cctacaacct gatcatggat
    10861 ccaccttggg aagcgcagta tgatctcggt tactaccgtc ctggcgacaa tatcccgacc
    10921 gacacatact ggctcccctt acacggctac ggcccaccgc tccgcggcag caactactga
    10981 gtgcaccaga tctacccttg accggaacac tcggtctggg attcaacgca taaagccaaa
    11041 aatcaattct tgaccccggg gcaaaggctc cggggttttt cgtttaccgg gacgttccac
    11101 tcgccctcgc cattaaaatt agtaaaacat aatataatca tttgattatt aagcttgaag
    11161 gtctcgatcg tcgatcatcg aatcgacatg attcccacaa atcgtgaatt tctggtgtat
    11221 ttgcgggatg gcggttgttg gccgggactc tattacataa gctgaagttg ttacaccgca
    11281 atcactccgg gatgagacga tcatcgacac catttgaaat gtgccaggca cgtcgcaatc
    11341 acttgagatg gagccaggac caggacgagg caagccaagc caacaaagtc aatgacgacg
    11401 gggatcgcgt cgatgccaca gcaacgcaac gcattggatt cggactggga caacgaacgg
    11461 gtcacggtcg gccgcaattt tctcagggac atggaggcgc tgtggtccga ggtgctcaag
    11521 ttggctgcag tggtcgaaga cgccctcaac cagagcgtcc aggggctatg cgacggtcgc
    11581 gttgatcttg cccatgagct caagggaaga aagcatgccg tggatcggtg ggaggtccag
    11641 atcgagcgcg agtgcgtacg cgtgcttgcc ctgcatcaac ctgtggcctc ggacctgcgg
    11701 cgggttgccg cggtcctcaa aatcaacggc gacctcgaac gcctttgcga cctggctcgg
    11761 catatcgcca agcgagtcaa gaagctggca gccgatcccc aggcctttcc cattccccag
    11821 acgctcgaaa acctggcact ggaagccctg tcccagattc acgccagtct cgatgccctc
    11881 acccagtcta acgtgtcgcg ggcccaggcc gttatcgccg ctgaccaagc agtggatcgt
    11941 aactacaggg cagtgcaaaa acagctcaag gccgagatca tccgcgatcc cgatcggatt
    12001 gacacctggc tgcggctggt aaatactgca cgcaacctcg agcgaatcgc cgatcacgcc
    12061 gccaagatcg ccgagtcagt gatctacctc aaggagggag aaatcttcag gcaccggacg
    12121 tcgggttctt cgcgggacgc cagcctcgtt tcactccccc ctgccgctag cagccagagt
    12181 taacagccac tgaccccgct gaatcaaatc ggcatgcgaa atatcttttc cggttcaccg
    12241 agtatcgcca agtggacagt gcgacggtcg cgggcgatga atccggcaga ctgatagagc
    12301 cgagccgcgg gtatgttgcg acaatcgacc gccagttcca gccgagag
//
LOCUS       DATAQL010001470         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2900046, whole genome shotgun sequence.
ACCESSION   DATAQL010001470 DATAQL010000000
VERSION     DATAQL010001470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2900046"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..1738)
                     /locus_tag="VHS97_25460"
     CDS             complement(383..1738)
                     /locus_tag="VHS97_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX3451627.1"
                     /translation="MAVIAPAPTQAVPGPRRQWLFGNLKEFGRDQLGTMTRWAREYGD
                     LVSARFGPRNVVFANHPDLVEEVLVNQNRKFIKHYRLRQTRRTLGQGLLTSEGEFWRG
                     QRRLAQPAFHRDRIAAYGRLMVDFTARLLESWHDGQVRDVQEDMMRLTLEIVAKTLFD
                     AEIAGDTALASAAMETLMRSFIARTGSLLAPPHWFPTPLNLRVERAISRLERILAKVI
                     AERRAGGEDRGDLLSMLLHAQDEESGRQMSDQQLRDEVMTLFMAGHETTANTLAWTWV
                     LLSAHPEAEARLHAELDQVLGDRLPTTADLPRLKYAESVINETLRVYPTVWVIGREAI
                     EPVELGGYSIPAGTTVFMPQWVIHRDGRWFQDPEAFRPERWEGGFLQRIHRYAYFPFG
                     GGPRICIGNNFALMEATLILATIARKYRLKLAPDANIVPLPTITLRPAYGVKAVLTER
                     R"
     gene            1866..3191
                     /locus_tag="VHS97_25465"
     CDS             1866..3191
                     /locus_tag="VHS97_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3451628.1"
                     /translation="MALEREGLSRRGFLDRSLATLTMAGLPLWYARDVVADDEEKNAA
                     ASKLKKLGPNDQIIMAAIGVGGQGTGIMKWAKKKPGVKFVAVCDLDDDRRKKAAKEVG
                     SDCHEYKDFRELLAKEDVNAVTIGTVDHWHALTSIAAMKAGCDVYCEKPLSLTIEEGK
                     AMVKAARKYDRVFQTGSQQRSDARYRLACELVRNGRIGKVHTVEARIGYNPIGGPFKT
                     SPVPEGLDWDFWKGPTADVPYIKERCHYEFRWWYEYSGGKLTDWGAHHNDIAQWGLGM
                     DGSGPVSVTASGTKPADKPDCYNCHPHFAVTYTYADGTHLVTSSDGENGNRFIGDKGW
                     IFVSRERIEASDKALLDEPLHGETVRLYVSNDHMGNFIDGTRTRHRPICDVEVGYRSV
                     TVCHLGAIALRVGIPLDWDPKAERFVGPCADKGNAMISREMRSPWRLEV"
     gene            complement(3328..>3771)
                     /locus_tag="VHS97_25470"
     CDS             complement(3328..>3771)
                     /locus_tag="VHS97_25470"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX3451629.1"
                     /translation="FFYLNESWSPSELKIFEQGNTLAKVIEADTDITNLQSDVFLFTA
                     SISGTVVSDQGHATDWWSSSRGVADVTVELEDSNGSALATTTTDGEGHYSFDQLNAVS
                     ATGDYMVRLIAPSGFKQASANPSTILISRGDISVRGVNFAIVRAN"
BASE COUNT          782 a         1176 c         1111 g          702 t
ORIGIN      
        1 cggaatgcca taactcgaat cgtttaatta catgagtgta tttagccaat cgctagccga
       61 ccgatgcgac gatgcgcttc accgctttca tcgctcgttt gacgtgtcat tcgtcgaaca
      121 caaccggaaa acggagtgcc aaacatcggc gcggcgagcc ttgccgatgc gagacctcaa
      181 tcgctgcaag gattggcagc tataaatgcc agacggtttc aatcggtcgg atccggcgtc
      241 aaaggattct ttcttaagaa aatttaagaa tccgagcgcg ttgactgggt tgtatcgtca
      301 cgccataccc atcttcgagc ggcgttaggg cgcgcgattg tcgaggatcg cacaagtcgg
      361 atccgatccg tagccatcgg cttcagcgcc gctccgtcaa aaccgccttg accccgtacg
      421 ccggccgtag agtgatcgtg ggcaatggga cgatgttggc gtcgggcgcg agcttgagcc
      481 ggtacttgcg ggcaatcgtc gccaggatca gcgtcgcctc catcagagcg aagttgttgc
      541 caatgcagat cctgggcccg ccgccgaagg ggaaataggc ataacggtga atccgctgca
      601 agaagccccc ttcccaccgt tcgggacgaa atgcctcggg atcttgaaac cagcggccgt
      661 cacggtggat gacccactgc ggcatgaaga ccgtcgtccc ggcgggaatg gagtaccccc
      721 ccagctcgac cggctcgatc gcctcgcggc cgatgaccca cacggtcgga taaacccgca
      781 atgtctcatt gatgacactc tcggcgtact tgagtcgcgg cagatcggcg gtcgtcggca
      841 aacggtcccc caatacctgg tcgagctcgg catggagccg ggcctccgcc tccgggtgtg
      901 ctgacagcaa cacccacgtc catgccaggg tattggcggt cgtctcgtgg cccgccatga
      961 agagcgtcat gacctcgtcg cgaagctgct ggtcgctcat ctgccgaccg ctttcctcat
     1021 cctgggcatg aagcagcatc gatagcaggt cgccccgatc ctcgccgccg gcgcgccgct
     1081 cggcaatcac tttcgcgaga atgcgctcca gccggctgat tgcccgctcg acgcgcagat
     1141 tcaaaggagt cggaaaccaa tgtggcggcg ccagcaggct accggttcgc gcgatgaagg
     1201 agcgcatgag cgtctccatc gccgcactcg caagggccgt atcgccggcg atttccgcgt
     1261 caaacaaggt cttggccaca atctccagcg tgagccgcat catgtcttcc tggacgtcgc
     1321 gcacctgccc gtcgtgccac gattcgagca gccgcgcagt gaaatccacc atcaaccggc
     1381 cataagcagc gattcgatcg cggtgaaacg ccggctgggc aagtctgcgc tggcctcgcc
     1441 aaaattcccc ttcgctggtc aagagcccct gaccgagcgt ccgccttgtt tgccggagcc
     1501 gataatgttt gatgaacttg cgattctggt tgaccaggac ctcttcgacc aggtcgggat
     1561 gattggcgaa gacgacattc ctgggtccga atcgggctga taccaagtcg ccatactcac
     1621 gcgcccagcg ggtcatcgtc cccaactgat cgcggccaaa ctccttcaaa ttgccgaata
     1681 gccactgccg ccttggtccg ggaacggcct gcgtcggggc gggcgcgata acggccattt
     1741 cagagcatcc tcatcttgcc tggagggtca aggaaccggc acgtgggcat ttcaccccgt
     1801 cgatcgtcag tgctatgata tcgcagtgat caatcatagc cgcaacttct tgggggaaat
     1861 tgacgatggc actcgagcgt gaaggattgt cgcgacgggg atttctcgat cgctcgctgg
     1921 ccacgttgac gatggctgga ctaccgctct ggtacgcccg cgatgtcgtc gccgacgatg
     1981 aagagaaaaa cgcggctgcg agcaagctca aaaaactcgg acccaatgac cagatcatca
     2041 tggcggccat cggcgtcggc ggccagggca ctggcatcat gaagtgggcc aagaagaagc
     2101 cgggcgtcaa gttcgtggcc gtgtgcgacc tcgacgacga ccgcagaaag aaagcggcca
     2161 aggaggtcgg cagcgactgt cacgaataca aagacttccg tgagctcctg gcaaaagaag
     2221 atgtcaatgc tgtcacgatc ggcacggtcg accactggca cgccctgacc tcgatcgcgg
     2281 ccatgaaagc gggttgcgac gtctattgcg agaagccgtt gtcgctcacc atcgaggaag
     2341 gtaaggcgat ggtcaaggcg gcgcgcaagt acgatcgcgt gttccagacc ggcagtcagc
     2401 agcggtccga cgcgcggtat cgcctggcct gcgagctggt gcgcaacggc cggatcggca
     2461 aggtgcacac cgtcgaggct cgtatcggtt acaatcccat cggaggccca ttcaagacca
     2521 gccctgtccc tgaggggctc gactgggact tctggaaagg ccccaccgct gacgtcccgt
     2581 atatcaaaga aagatgtcac tatgaatttc gctggtggta tgaatacagc ggcggtaagc
     2641 tcaccgactg gggcgcccac cacaacgaca tcgcgcagtg gggcctggga atggatgggt
     2701 caggaccggt aagcgtcacc gcgtcgggca caaagccggc cgacaagccc gactgctaca
     2761 actgccaccc ccatttcgct gtgacctaca cctacgccga cggcacccac ttggtcacga
     2821 gtagtgacgg cgaaaacggc aaccgcttca tcggcgacaa aggatggatc ttcgtcagtc
     2881 gcgagcggat cgaggccagc gacaaagcgc tcttggacga accactccat ggggagaccg
     2941 tccggctcta cgtctcgaac gaccacatgg gcaacttcat cgacgggacc cgcacgcgcc
     3001 atcggccgat ctgcgacgtc gaggtcggct accgctcggt gacggtctgc cacttgggcg
     3061 cgatcgcatt gcgagtgggc atcccgctcg actgggaccc caaggccgaa cgtttcgtag
     3121 gtccctgcgc cgacaaaggc aacgccatga tttcacgcga aatgcgctca ccgtggcggc
     3181 ttgaggtcta gatcagcaac ccctccgcgc tcgatcgcgc tccgcgtccc acgtcccggg
     3241 agggcgagcc tccgtgcgag ccagatccac gcgggccgag gctcattccg gctcggacag
     3301 agcctcgccc tcccaccaat cggctcgcta atttgccctt actatcgcaa agttcactcc
     3361 cctcacgcta atgtcgccgc ggctgatcag aatcgttgag ggatttgccg acgcttgctt
     3421 gaatccggac ggcgcgatga ggcgcaccat gtaatcgccg gtggcgctca ccgcattgag
     3481 ttggtcaaag ctgtaatgcc cctcgccgtc agtcgtggtc gtagcgagcg cgctcccgtt
     3541 cgaatcctca agctcgacag tgacgtcggc aacgccccgt gacgacgacc accaatcggt
     3601 ggcatggcct tggtcgctaa ccacggttcc actgatcgag gccgtgaaca agaagacgtc
     3661 gctttgtaga ttagtgatgt cggtatcggc ctcgatgact ttggccagcg tattcccctg
     3721 ctcgaagatc ttcagctcac tcgggctcca cgattcgttc aagtaaaaga a
//
LOCUS       DATAQL010001471         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5037559, whole genome shotgun sequence.
ACCESSION   DATAQL010001471 DATAQL010000000
VERSION     DATAQL010001471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5037559"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(631..1662)
                     /gene="holB"
                     /locus_tag="VHS97_25475"
     CDS             complement(631..1662)
                     /gene="holB"
                     /locus_tag="VHS97_25475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX3451630.1"
                     /translation="MSWQSVRGHDRVVDSLQSSLRQGRFPHALLFVGAQGIGKRTFAR
                     KLAQALLCETRPADLLDPCGICPGCVQVEGGTHPDSIEVGRPEDKHELPISVIRELCD
                     QFALKPARGMRKVAIVDDADYLNEEASNAFLKTLEEPPPGALLILIGTSAELQLETIV
                     SRCQLVRFDPLPESEIAALLLEKGVASNADDAARLAALGEGSVSRAIGLADGELERFR
                     RSLIDEVAAEHGFDPPALAHRLHAFIKQAGKESVDQRGRASLLIGELARFFRGVLWQT
                     AGLAPPCPDPVDRRCVSNLAKRLEPEDVFVLADRCVLADYHVQRRVYMPLILESLMHD
                     LGKMINSRG"
     gene            complement(1634..2335)
                     /gene="tmk"
                     /locus_tag="VHS97_25480"
     CDS             complement(1634..2335)
                     /gene="tmk"
                     /locus_tag="VHS97_25480"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX3451631.1"
                     /translation="MPSSGTHAHPGLFLVLDGPDGGGKSTQAARLADWLCSLCRDVVT
                     CRDPGGTALGERLRAILLERAAISISLRSEMLLYMASRAQLVEEVIAPALAAGGVVIS
                     DRYLLANIVYQGSAGGLLEEEIALVGMVATGGLLPDLTIVLDIAPDTASARVGPARDR
                     IEDRPLFYRERVRAGYLAAARGESDPGATPGEPMGCPYYPAPIVLIDASNDPDTVFRE
                     IKREVERVLAIRPRP"
     gene            complement(2328..2795)
                     /locus_tag="VHS97_25485"
     CDS             complement(2328..2795)
                     /locus_tag="VHS97_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX3451632.1"
                     /translation="MTAFHERSAGVIPYRWGDDRVPLYLILHSATVRNPRAKWEFPKG
                     GVEVGESTRQAAAREFREETSLVDWSFRDGFEQTLTYTYIRRGRKVVKTVTYYVVEVR
                     DPTPLACSPEHDVDPFGQWYRWGSFDEITLLLYHTKIRQVFADADGWLRGKDA"
     gene            complement(2792..3190)
                     /locus_tag="VHS97_25490"
     CDS             complement(2792..3190)
                     /locus_tag="VHS97_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX3451633.1"
                     /translation="MPPASVEIDGGTEDKTGFTMRLAAVVLPDLGTGPDTPIVISHWF
                     AACGDRVWEGERLVEVLVGPATFDVSSPVSGRLAEIRGWEDDRVVAGAVLGLIATADD
                     DAYDDLASEPKRDRRRGQSGDEPPSSRPTT"
     gene            complement(3317..>3509)
                     /locus_tag="VHS97_25495"
     CDS             complement(3317..>3509)
                     /locus_tag="VHS97_25495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451634.1"
                     /translation="SFNQWLAGIRGSYDVKPPREYTVNPGKPGELWLRDRYVVLDLVP
                     RGMDEQRVTGELSRSKAVR"
BASE COUNT          678 a         1137 c         1065 g          629 t
ORIGIN      
        1 gtctgcaccg actccaacgt gacccaggcc tcgaactggc agaaatctct gagtcggatc
       61 agacgagcgt cggtcactcg catttccatc gtccttgatt catggtaact ggtacattct
      121 atggacccgg gaggccaccc acgtgtcgat taccagccgg acgcttcctt acctgtcggg
      181 ctggaaagtg ggtcgcttgt tcaaccacat cggtaccaca acgtcaagag caagagcgct
      241 gattcgtggt acaggcgctc ttggggaccc gtacgccacc tcgcttgcgt cgcctcggca
      301 tcgcccagct atgggccggc agtaggatcg gcgagcgccc tgctcacaat aagtggacct
      361 gaccggaacg gtggttgcag attttttccg cgaatgccga gattgagtcg atcaatgtca
      421 ccagcacgtt ggtcggcttg acgtcgcgat gaacggtcac tttctggtgg gcgtgttgca
      481 taacctggca gaccgggata aacagctcca ggcgagttca atccagcgtc acggcggggc
      541 aagcgacctg gaagtcatcg aggtcctcga aactcagccc acagccctga atgtccagcc
      601 ggttgagctt ggggagggcc ttcccaagtc tcatcctcgg gaattgatca tttttcccag
      661 gtcgtgcatg agcgactcga gaatcaaagg catgtaaacc cggcgctgca cgtggtagtc
      721 ggcgaggacg cagcggtcgg ccaggacaaa aacatcctcg ggctcgagac gcttggccag
      781 attgctcacg caacggcgat cgaccgggtc ggggcaaggg ggtgccaggc cggccgtttg
      841 ccagaggacg ccgcggaaaa agcgtgccag ctcgccgatc aaaagactgg ctctgccgcg
      901 ctggtcgact gattccttac ccgcctgctt gatgaacgcg tgcagccgat gggccagggc
      961 agggggatcg aagccatgtt cggcggcgac ctcgtcgatc aatgaccggc gaaagcgctc
     1021 gagctcgccg tcggctagcc cgatcgcgcg gctcacactc ccctcgccca gcgcggccag
     1081 ccgcgcggca tcgtcggcgt tactcgccac ccccttttcg agtagcaacg ccgcgatctc
     1141 ggactcaggc agcgggtcaa aacgcacgag ctggcagcgc gagacgatcg tttcaagctg
     1201 gagctcggcc gaagtgccga tcaggatcaa gagcgctccc ggcgggggct cttccagcgt
     1261 cttcaagaat gcgttggacg cctcttcgtt caagtaatcg gcatcgtcaa caatcgccac
     1321 ctttcgcatt ccgcgcgccg gcttgagcgc gaattggtcg cacaactcgc ggatcacgct
     1381 gatcggcagc tcgtgcttgt cttcgggccg gccgacctcg atcgagtcgg gatgcgtgcc
     1441 gccctcgacc tgaacgcagc ccgggcagat cccgcatgga tcgagaaggt cggccgggcg
     1501 ggtttcgcac agaagtgctt gcgcgagctt gcgagcgaag gttcgcttgc caatcccctg
     1561 ggcccccacg aaaagcagtg cgtgcgggaa acgcccttga cgcagactgc tttgcaacga
     1621 gtcgaccacg cgatcatggc cgcggacgga ttgccaggac acgttcaacc tcgcgtttta
     1681 tttctctgaa cacggtgtcg ggatcgttgg aggcatcgat caagacgatg ggggcgggat
     1741 agtacgggca acccatcggt tcgcccggcg tcgcccccgg gtccgactcg ccgcgcgccg
     1801 ccgcaagata gccggcgcgc acccgctctc gataaaacag gggccggtcc tcgatccggt
     1861 cgcgcgcggg cccgacgcgg gcgctcgcgg tgtccggagc gatgtcgagc acgatcgtca
     1921 gatcgggcaa aaggccgccg gtcgccacca tgcccacgag cgcgatttcc tcttcgagca
     1981 ggccgcctgc acttccttgg tagacaatgt tggccagaag gtaacggtcg gagatcacga
     2041 caccccccgc ggccagggcc ggggcaatga cttcctcgac gagctgcgcc cggctggcca
     2101 tgtagagaag catttccgac cggagcgata tactgatagc ggctcgctcg agcaagatgg
     2161 cgcgcagccg ttccccgagc gccgtcccgc cgggatcgcg gcaggtcacc acgtcccggc
     2221 aaagactaca cagccaatcc gccagccgcg ctgcctgcgt cgacttgccg ccgccgtcgg
     2281 gaccatcaag caccaggaag agaccgggat gagcatgggt gccagagcta ggcatctttt
     2341 cccctgagcc agccgtcggc gtcggcaaac acctggcgga tcttggtgtg gtataacagc
     2401 agtgtgatct cgtcaaagct gccccagcga taccactggc caaacggatc aacgtcgtgc
     2461 tccggcgaac aggccagggg cgtcgggtcg cgaacctcga ccacgtaata cgtaaccgtt
     2521 ttcacgactt tacggccgcg ccggatgtac gtgtaagtga gcgtttgctc gaagccgtca
     2581 cgaaacgacc agtccacgag ggaagtctcc tcgcgaaact cgcgcgcggc cgcttggcgg
     2641 gtcgactcgc ccacttccac gccccccttg gggaattccc acttggcgcg cgggttacgc
     2701 accgtcgcgg agtgaaggat caggtacaac ggcacccgat catcgcccca gcgataggga
     2761 atgacgccgg cggatcgctc atgaaacgcg gtcatgtcgt ggggcgactt gacggcggtt
     2821 cgtcaccgct ttggccgcgc cggcgatcgc gcttcggttc cgaagcaaga tcgtcatacg
     2881 catcgtcgtc ggccgtggca atcagaccga gaaccgcacc cgccaccacg cgatcgtctt
     2941 cccagccgcg gatctccgcc agacggccgg acacgggcga ggaaacgtcg aaagtcgctg
     3001 gtccaaccag gacttccacc aaccgttccc cttcccagac ccggtccccg cacgcggcaa
     3061 accagtgact gatgacaatc ggtgtgtccg gaccggtccc caggtcgggc agaaccactg
     3121 ccgccaaacg catggtgaac cccgtcttgt cttctgttcc accgtcgatc tcgaccgagg
     3181 ccgggggcat cgcggacgat acaccttatg gaacacctcg atcacttcaa ctctagctta
     3241 tctggcctgt cgccttttgg ccagatcgat gtcgagtcgc cgactaccgc cgagcatttt
     3301 tcgcaggtca atttccctaa cgaacggctt tggaacgact taactcgcct gtcactcgct
     3361 gttcatccat ccctcgcgga acgaggtcca gcacgacgta ccggtcccgc agccagagtt
     3421 cgccgggctt gcccggattg accgtgtact cgcgaggagg cttgacatcg tatgagccgc
     3481 gaatccctgc taaccattgg ttgaacgag
//
LOCUS       DATAQL010001472         4286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_2137213, whole genome shotgun sequence.
ACCESSION   DATAQL010001472 DATAQL010000000
VERSION     DATAQL010001472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137213"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VHS97_25500"
     CDS             complement(<1..661)
                     /locus_tag="VHS97_25500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451635.1"
                     /translation="MSMSLRMLTASWLAVVLIGLLGFKLVHDTDIFWQVKLGQIMLEE
                     GQIPIADRFTYTHAGEPVPPIYCLAQILLAVLYKLGGWHLARAGHQLALVGSLVAAAA
                     TCRRDRTSHFSVIVAMTIGFVLMLSNADFRPQSFGVLGFAFFLALARSGAPFWFKLLV
                     APAILVIWQNMHPSVLMGAVAVGGLAVADYHDRRHIHVNPWEQVILALLAFVSQFATP
                     LG"
     gene            1074..4109
                     /locus_tag="VHS97_25505"
     CDS             1074..4109
                     /locus_tag="VHS97_25505"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3451636.1"
                     /translation="MAVGSFEVEPKTRVIDVKSRVVAPGFIDLHTHSDPRISEPSTRL
                     NQNYLTQGVTTVVTGNCGLGVSDVAKYLAAIDAHGAGTNVIHLIPHGSVRSTVMGTAE
                     RPPTASELERMKQLIERGMDAGAWGLSSGLIYVPGRYADTNELIELARVARRRGGIYA
                     SHIRNEGARLLQSIDEAIAIGKGADIPVHISHLKASGKAYWGTVGPALGRIARARETG
                     QVVTADQYPYIASSTQLAAMVVPHWAIQGSSDEFARLAADPARGQSLRREIQQALDER
                     DGGASIRIARYAAHPDWAGLDLNTIARHEQKTPLEIIIEIQRNGGAQAISFGMSEDDV
                     REVMRHEFVATASDGATHSPSRVGDHPHPRAYGTFPRKIRYAVDDKTLSLEQAIRSCS
                     GLPAEILGLPDRGVIRPGAFADIVVFDPSTFRDAATFDHPTRYAPGVSHLFVNGTALI
                     DEGKLEVSANPKGKLPGRALRLALDGPADLIIKVKRIWTGDHANPWAEALAARGGAIA
                     AVGNIADVVRFRGPLTRVIDRPDAFATPGLIDAHGHMESLGASEEELDLRGVASLDQV
                     VERVQERISATPGDSWITGRNWDQSLWPGGAFPTASVLDAVSPKRPVWLERVDGHAGW
                     ANSEAMRRAKVNKDSMAPSDGQIIRDSQGQPTGVFIDGAMSLVGRAVPVPTLNDVKRR
                     LLKAQRTALRNGLTGVHDAGIGKLVAEAYRELDRQGELAVRIYGMASPPAGGEVAFAS
                     QRPQFVATPSKFELRAIKLFVDGAMGSRGGLLFRPYSDDPGNSGLMLIDPKVLEATTA
                     AALKHGWQVCTHAIGDKGNALVLDAYAAALKAVPEARNPRLRIEHAQVVRKEDVSRFA
                     QLGVIASMQPSHASDDMRWADARLGPGRVDGAYAWRWFVDAGVTLAHGSDFPVEIVSP
                     WWGIYAGVTRQDPQGKPPGGWHPEQRLSLEETLRGFTAGAAFAAFAEDKVGILKPGLR
                     ADLTIVDRDLFLAENTDLLATQVLMTVVDGVVVYDGAKP"
BASE COUNT          856 a         1283 c         1356 g          791 t
ORIGIN      
        1 cgcctaacgg agtggcgaat tgggatacga acgcgagaag agcgagaatg acctgctccc
       61 aaggattcac atgaatgtgg cggcgatcat ggtagtcggc cacagccaat ccgccgaccg
      121 cgacggcgcc catcaaaacc gacggatgca tgttttgcca gattaccaga atggctggtg
      181 cgaccaagag cttgaaccaa aatggcgcgc cggagcgtgc cagtgccaga aaaaatgcga
      241 agccaagaac gccgaagctc tgaggccgaa aatcggcgtt tgaaagcatc aggacgaaac
      301 cgattgtcat cgcgacgata acactgaaat gtgatgtgcg atcgcgccgg cacgtcgcag
      361 cggccgccac caatgagccg accaaggcga gttgatgacc ggctcgcgcc agatgccatc
      421 cgccgagctt atagagcacc gcaagcaaga tttgagcgag gcagtaaatc ggcggaactg
      481 gttcgccggc gtgggtgtac gtgaaccgat cggcgattgg gatctggccc tcctccagca
      541 tgatctggcc gagctttacc tgccagaaga tgtcggtgtc gtgcacgagt ttgaagccta
      601 gcaaaccgat aagcacgacg gctaaccacg atgcggtaag catgcgcagc gacattgaca
      661 cttagctttc tattttggtg tttgcgggca cgcccctcca gcgcaaggat agtacaggaa
      721 aaaagagaca aagcgcggac gtgcgaagat tgtaggggcg cgcatcgaca ggccctttgt
      781 tccgctacac tgaaacaacg caagactttg tagctcaggg ctgcgaatcc gtacggaggt
      841 tcacgatggt gatggtcggt cgcagtgttt gggggttgca tttactcgct cgtgtcgtag
      901 ccgcaatcgt ttgttcgaca gtttgtcgcg gagctgccga ttcactcggc ccggctgatc
      961 gcgcgccgaa ggagcccatt catgctgact ttgtcataaa gggggggacg cttatcgacg
     1021 gcactggggc gccagcgcgg ccggccgatg tcgctgtagc aggcggtcga atcgtggcag
     1081 tcggttcctt tgaagtcgaa cccaaaacac gcgtcatcga cgtaaaatcg cgcgtggtcg
     1141 cgccgggatt catcgatctg cacactcact cagacccccg gatcagcgag ccgagcacgc
     1201 ggctcaacca aaactatctg actcaaggtg tgacgaccgt cgtcaccggt aactgtgggc
     1261 tcggcgtatc cgacgtcgcc aaatacttgg cagcgattga tgcccatggt gcaggcacca
     1321 acgtgatcca tctcatcccg cacggttcgg tccgttcgac cgtcatgggc actgcggaac
     1381 gtccgccgac cgcgtcggag ttggaacgaa tgaagcagct gatcgagcgg ggcatggacg
     1441 cgggtgcctg gggactttca agcggcttga tatacgtccc cggccggtat gccgacacca
     1501 atgagctcat cgagcttgca agagtcgcca ggcgccgggg gggcatttac gcgtcgcaca
     1561 tccgcaacga aggcgccagg cttctccagt cgatcgacga agccattgcc ataggcaagg
     1621 gcgcggacat cccggttcac atctcgcacc tgaaggccag cggcaaagcc tactggggta
     1681 cggtcggacc ggccctcggt cggatcgcgc gtgcgcgtga gaccgggcaa gttgtcacgg
     1741 ccgaccagta tccttatatc gcgtcgagta cacaactcgc cgcgatggtc gtgccgcact
     1801 gggcgattca gggttcgtca gacgaattcg ccagactggc agcggacccg gcccgtggcc
     1861 agtcgcttcg tcgcgaaatc cagcaggcac tcgacgaacg cgatggcggt gcctcgatcc
     1921 ggattgcgcg gtacgcggct cacccggact gggccgggct cgatctgaac acgatcgcac
     1981 gccatgagca aaagacgccg ctagaaatca ttatcgaaat tcagcggaac ggcggtgctc
     2041 aggcgatcag ctttggcatg agcgaagacg acgtccgcga agtgatgcgg catgagttcg
     2101 tcgcaaccgc gtccgatggc gctactcact cccctagccg ggtgggggat catccgcacc
     2161 cgcgcgccta cgggacgttt ccgcgcaaaa tccgctacgc cgttgatgac aaaactctct
     2221 cgctcgaaca ggcgattcgt tcgtgctctg gcctgccggc ggaaatcctc gggctccccg
     2281 accgaggcgt aatcagaccc ggggcgtttg ccgatatcgt cgtcttcgat ccgtcgacgt
     2341 ttcgcgatgc tgcgaccttc gaccacccga cgagatacgc accgggagtc agtcacctat
     2401 ttgtcaacgg aaccgccttg atcgatgaag gcaagcttga ggtctcggcg aaccccaaag
     2461 gcaagctccc tggccgtgca ttgcggctgg ccctcgacgg cccagccgat ttgatcatca
     2521 aggtcaaacg catatggaca ggggatcatg caaacccttg ggccgaggct ctggcggcgc
     2581 ggggcggtgc gatcgccgcg gtcggtaaca tcgccgacgt cgtgcgattt cgcggaccat
     2641 tgacccgcgt gatcgaccgg cccgacgcct ttgcgacgcc ggggttgatc gacgcccacg
     2701 ggcacatgga gtcgctaggc gccagcgagg aagagctcga cttgcgcggc gtggcatcgc
     2761 tcgatcaggt cgtagagcgc gtgcaggaaa ggatcagtgc aaccccgggc gattcgtgga
     2821 ttaccggacg aaactgggac cagagtctct ggccaggcgg tgcctttccc acagcgtccg
     2881 tactcgatgc cgtgtcgcca aagcggcctg tctggctcga gcgggtcgac ggccacgccg
     2941 gctgggccaa ctccgaggcg atgcggcgag ccaaggttaa caaggactcg atggccccat
     3001 ccgacggcca gatcatccgt gattcccaag gtcaaccgac cggcgtcttc atcgacggcg
     3061 cgatgagttt ggtcggccgg gccgttccgg tacctaccct gaacgacgtg aagcgaaggt
     3121 tgctgaaggc gcaaaggacc gctttgcgca atggactcac gggcgtgcac gatgccggta
     3181 tcggaaagtt agttgctgag gcctatcgcg agctcgatcg ccagggggag ctggcggtcc
     3241 gtatttacgg catggcatcg cccccggcag gaggcgaagt tgcgtttgca agccagcggc
     3301 cgcaattcgt tgccacgccg tccaagttcg agctgcgagc catcaagctt ttcgttgacg
     3361 gtgcgatggg ttcccggggc gggctcttgt ttcgtcccta cagcgacgat ccgggtaact
     3421 ccgggctcat gctgatcgat cccaaggttc tcgaggcgac gacggccgcc gctctcaagc
     3481 atggctggca ggtgtgcact catgcgatcg gcgataaggg aaatgccctc gtgctcgatg
     3541 cctatgccgc cgccctcaaa gcggtccccg aggcacgtaa cccacgtcta cggatcgaac
     3601 acgcccaggt cgtgcgcaag gaagacgttt ctcggtttgc gcaactgggg gtgatcgcct
     3661 cgatgcaacc ctcgcacgcg agcgacgaca tgcgctgggc cgatgcacgg ctgggtcccg
     3721 gccgtgtcga cggggcttat gcgtggcgct ggtttgtcga cgcaggcgtg acgctggcgc
     3781 acggcagcga tttcccggtc gagatcgtca gcccgtggtg ggggatctac gcaggcgtga
     3841 cgcgccagga cccacagggg aagcctcctg gcggctggca tccggaacag cgcctttctc
     3901 tggaggaaac cctcaggggc tttacggccg gcgctgcctt cgcggccttc gccgaggaca
     3961 aggtcgggat tctcaagccc gggctccgcg ccgatttgac catcgtcgac cgcgacttat
     4021 tcctcgccga gaataccgat ttgctcgcga cccaagtctt gatgacggtt gtcgatggcg
     4081 tggtcgtata cgacggcgcc aaaccctgaa aattttcagc gagccgacgc aagacgattc
     4141 ctgcgccggc tcgcggttgc tttcgatcat ttcggcagtt cgtcgtactt gggcgtgacc
     4201 ttgcggatat tgggctcgac actggcgacg atacgtccag ttgcccagcc cagtgcgccc
     4261 agaagcagcg tctgataatt tgggtt
//
LOCUS       DATAQL010001473         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_5037866, whole genome shotgun sequence.
ACCESSION   DATAQL010001473 DATAQL010000000
VERSION     DATAQL010001473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5037866"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="VHS97_25510"
     CDS             <1..1776
                     /locus_tag="VHS97_25510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451637.1"
                     /translation="PYWSANLKQVPSAWGIDGWPENQGPFGLLFFNPKLQEGYKAWLK
                     ALLTRPNPYTKIPLAQDPALAIIQLQNEDSLLFWTAQNIKGKQAELLGRQFGDWLQAK
                     YGSLGAAFKAWANDKVQEDRPDEGIVGLLMVWEWTQAREGGRKRRLDDQLQFYAEKMY
                     GFNREIERYLRAELGCKQLVNAGNWKTADAVKLDDAERWSYTANEVLAVNRYYSPVHI
                     GPDRGWRIDPGDSFEDASVLLRPRELPVNLKQAAGHPMMITESHWVPPLGYQSEGPFL
                     VATYESLTGVDVFYWFATGESEWSNQDRGAWDSASRAKWAIATPMVLGQFPAAALLFR
                     KGYLNEGQPVLEEHRSLRQIWERVPPILAEDPGYDPNRDLGDTARRSGLKGSVDPLAF
                     LVGPVKVHYDSEPAQTKLADLSGFIDHKNKIVRSNTGQVRFDYEHGVCTIDGPAAQGV
                     TGFVKTVAPVELNTVTIDSENTYASVLVVSLDGAALSQSRSALIQVGTRARPTGWVQR
                     TATFAGDDGKQTFHGKQVVSTGTMPWVVEDTKMTVTVKNPELRKAKLLDINGNVRGDL
                     TVATSAGAIKFSLPKDAMYIVLQSE"
     gene            1836..2537
                     /locus_tag="VHS97_25515"
     CDS             1836..2537
                     /locus_tag="VHS97_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3451638.1"
                     /translation="MGIRVLVVEDEATIADFLIRGLREEGYVVEHAADGGTAWNMLRS
                     SEWDLIVLDWWLPDQDGLTLLQRFRQIGRHTPVLFLTARDAVSDRVRGLDAGADDYLA
                     KPFAFEELLARIRALVRRRDSVEHMTIVYRDVRVDLAAQRAERAGHALPLTAKEHALL
                     VFFVRHPGQILSRTRIYEHVWDERYDGLSNTLEVHVKELRRKLEAYGPRLIHTLRGRG
                     YLLGDLPEAHQGDES"
     gene            2534..>3216
                     /locus_tag="VHS97_25520"
     CDS             2534..>3216
                     /locus_tag="VHS97_25520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451639.1"
                     /translation="MSLATRVSGFFLAALALALIGFSTTLYLLARSHFQRDLDERLVT
                     ALDVLSGSAKVTPGRVEWEPLARPMIKSTTPQEDPVCWVVSDGQAKVLENSWPDLTNE
                     DIVSVSSLSPDFGHNHGTFVDRAGRHWRLAVQGIPAAPSSASPPDRDEIERDESEEDS
                     GRGQSGTSSLGGSPSLILAAGAPSEPMDVSLRNVALTLAGVSIAIWLLAALVGRRVCD
                     RALSPVTRMA"
BASE COUNT          642 a          938 c         1002 g          634 t
ORIGIN      
        1 ccatattggt ccgctaacct caaacaagtc ccatcggcct ggggaatcga tggctggccg
       61 gaaaaccaag ggccgtttgg tctattgttc ttcaatccca agcttcaaga gggatacaag
      121 gcctggctca aggcattgct cacacgcccg aatccgtaca cgaaaatccc tctggcgcaa
      181 gaccccgcgc tggcaatcat tcagctccag aatgaagaca gccttctgtt ctggacggcc
      241 caaaacatca aggggaaaca ggccgagctc ctgggtcgcc agtttggtga ttggctccaa
      301 gcaaaatacg gctcgcttgg cgccgcgttc aaagcttggg cgaacgacaa ggtccaggaa
      361 gataggccgg atgagggtat cgtgggcctg ctcatggtct gggagtggac ccaggctcgc
      421 gaggggggtc gcaagcgtag gctcgatgac cagcttcagt tctatgccga gaaaatgtac
      481 ggcttcaatc gcgaaattga gcgctatctc cgcgcagagc tcggatgcaa gcaattggtc
      541 aacgccggca actggaagac ggccgatgcg gtgaagcttg acgacgccga gcgctggtcg
      601 tacacggcga atgaggtttt ggctgttaac cgctattaca gcccggtaca cattggtccg
      661 gaccgcggct ggcggatcga tcccggcgat tccttcgagg acgcgtcggt attgcttcgg
      721 ccccgagagc tgcccgtgaa cctcaaacag gccgccggcc acccgatgat gatcaccgag
      781 agccactggg taccaccctt gggttatcaa tcagagggac cgttcctcgt ggcgacttac
      841 gagtcattga ccggcgtgga tgtattctac tggtttgcga cgggggaatc cgaatggtca
      901 aaccaggatc gaggggcgtg ggactctgct tcgagagcca aatgggcaat cgcgacgcct
      961 atggtcctgg gccagtttcc cgccgcagcg ctcctttttc gtaaaggcta cttgaatgaa
     1021 ggccaaccgg tcctggaaga gcaccgctca ctgcggcaaa tctgggagcg tgtaccgccg
     1081 attctggcgg aggatcctgg atatgacccc aatcgcgacc tgggtgacac ggcccgccgc
     1141 tccggtttga aggggagtgt tgatccgctc gcgtttttgg tcggaccagt caaagtgcat
     1201 tacgattccg aacccgcgca gacgaaatta gccgacctca gtgggttcat cgaccataaa
     1261 aacaagatcg ttcgctcgaa cacaggccag gttcgatttg actatgaaca tggcgtttgc
     1321 acgatcgacg gtcccgccgc ccagggcgtt acaggttttg tgaagacggt cgcgcccgtc
     1381 gagcttaaca ccgtaacgat tgattcggag aacacatatg cctcggtgct ggtggtttct
     1441 ctggatggcg cggctttgtc acaaagccgg agcgccttga ttcaggtcgg gaccagggca
     1501 cgaccgaccg ggtgggtcca gcgcacggcc actttcgcgg gcgacgacgg caagcaaact
     1561 ttccacggca agcaagtggt cagcacgggt acgatgccct gggtcgtgga agacacgaaa
     1621 atgacagtga ccgtgaagaa cccggaactc aggaaggcaa agttactcga cattaacggc
     1681 aatgtgcgcg gcgacttgac cgttgccact tccgccggcg ccatcaaatt cagcttgccc
     1741 aaggacgcga tgtacatcgt tctccagtcg gaataaaccc gcgccgcatg agacgcgaat
     1801 actccggggc gagcaggtcg agatcgaggt cagtcatggg cattcgcgtt ttggtggtcg
     1861 aggatgaggc gacgatcgct gatttcttga tccgagggct gcgagaagaa gggtacgtgg
     1921 tcgagcatgc cgccgacggt gggaccgcat ggaacatgct caggtccagc gagtgggacc
     1981 tgatcgtcct ggattggtgg ctgcccgacc aggacgggtt gacactcttg caacgttttc
     2041 gccagatcgg ccgccacacg ccagtgctgt tcttgacagc ccgtgacgcg gtatccgacc
     2101 gggtacgggg tctcgacgcc ggggccgacg attacctggc caaaccgttt gcgttcgaag
     2161 aactcctggc ccgaatccgg gctttggttc gccggcggga ttccgtcgag cacatgacga
     2221 tcgtataccg agacgtgcgg gtggatctgg ccgcacaacg ggccgaacgt gccggccacg
     2281 ccctaccgct gacggccaag gagcacgcgc tcttggtatt cttcgtgcgg catcccggcc
     2341 agatcttgtc gcggacgcgg atttacgagc acgtgtggga cgagcggtac gacgggctct
     2401 ccaacacgct ggaggttcac gtcaaagagc tgcgccgcaa gctcgaggct tacggcccgc
     2461 gcttaatcca cactttgcgg ggccggggtt atctgctggg cgacttgcct gaggcacatc
     2521 aaggagatga gtcatgagtc tggccacacg cgtcagcggt ttttttctgg ccgctttagc
     2581 ccttgccctc atagggtttt ccaccacgct ctatttgctg gcgcgatccc attttcagcg
     2641 ggacctggac gagcggctgg tgacggccct cgacgtcctc tcgggctcgg ccaaggtgac
     2701 accaggacgg gtcgaatggg agcctctggc ccggcccatg attaaaagca ccaccccgca
     2761 agaagatccg gtctgctggg ttgtctctga cggtcaggcc aaggtactcg aaaactcttg
     2821 gccggatctg accaacgagg acatcgtcag cgtctcgagc ctaagccctg atttcggcca
     2881 caaccatggc acattcgtcg accgcgccgg caggcattgg cgcctggcgg tgcagggcat
     2941 tccggccgct ccgtcttcgg catcaccacc cgatcgcgat gagattgaac gcgatgagag
     3001 tgaagaggac tccgggcgcg gtcaatccgg cacgtcgtcg ttgggcggct ctccctcgtt
     3061 gatcctggcg gccggcgccc cctccgagcc catggacgtc agtcttcgaa acgtggcctt
     3121 gaccctggcg ggtgtatcga ttgcgatctg gctcctcgcg gccctcgtgg gtcggcgggt
     3181 ttgcgatcgg gcgctctcac cggtaacgcg catggc
//
LOCUS       DATAQL010001474         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4732199, whole genome shotgun sequence.
ACCESSION   DATAQL010001474 DATAQL010000000
VERSION     DATAQL010001474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4732199"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VHS97_25525"
     CDS             <1..918
                     /locus_tag="VHS97_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3451640.1"
                     /translation="RVVAFVNAFHGRTYGAMSLLGSKPMQRRGFSPLVPDIHHARYGD
                     VESVRALFRTICPPDELAAIFVEPIQGEGGYIVPPPDFLPGLRRLCDEHGVLLVFDEV
                     QSGMGRTGKMFAAEHWGVAGDIMCLAKGIANGLPLGAIVAKASVMDWPSGSHASTFGG
                     NPVACAASLATIKLLETRYRANAERRGHQLHAGLTALAHRFSMIREVRGLGLMIGAEI
                     QSEAGEPDATLRDQIVEIAFRRGLLLLPCGPSTVRFCPPLCVNRRQIEIGLELFSSTL
                     EAALGEMKRGEPDGADSIPELEELAQEPA"
     gene            986..2413
                     /locus_tag="VHS97_25530"
     CDS             986..2413
                     /locus_tag="VHS97_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451641.1"
                     /translation="MTVIAESAAPVVFDWKRWPRTELLVDELIETALSGNAFATELAG
                     RMSRETGTRFKDWVDHLVIADEEGLSHRLEEAGFERQRAAYAVGIPVFAHPGGIFPRI
                     ALASRQKRSNGAPGGVVSEVAIKVESVADFSRAHDLGLEMVGYSMGPYRVGRVPGDKT
                     ALAVVERRGYLGFEPFPGELAREGRMKPHAARDALAARDLWQARRRSFDDDLEGFDAT
                     EATLERVIELAGSTDLACHLVFEVERDYWQSRNKAARAQKARQDRLGLGWANHDHHTF
                     RCSRRFFSRVIGMFSRLGFALRERFHAGEHAGWGAQIVEHPVTGIVIFADLDLAPEEA
                     TADFAHQSLPDLPRPGTVGLWVALHGESILEAGMHHLEAQFDFDALRDGLKEDAGIET
                     MPPFSNFPFLRQAFTAGERWPVAKHRVDRALSQGWITAVQHEKFLREGAIGSHLENLE
                     RREGYKGFNQQAVSAIIAATDPRLH"
BASE COUNT          464 a          769 c          851 g          495 t
ORIGIN      
        1 cgggtagtgg cgttcgtcaa tgcgtttcac gggcggacct acggagccat gtcgctcttg
       61 gggtccaagc cgatgcagcg ccggggtttt tctcccctcg tccccgacat ccatcatgct
      121 cgttacggcg acgtcgaaag cgtaagagca ctcttccgga cgatctgccc accggacgag
      181 ctcgcggcga tttttgtcga gccgatccag ggtgaagggg gctacatcgt gcctccgccg
      241 gattttctgc ccggactgcg taggctttgc gacgagcacg gcgtgctctt ggtcttcgat
      301 gaggttcaaa gcggcatggg tcgcaccgga aagatgttcg ccgccgagca ctggggggtc
      361 gccggggata tcatgtgtct ggccaagggt atcgccaatg gcctgccctt gggagcgatc
      421 gttgccaagg cctcggtcat ggactggccc agcggcagcc acgcgtcgac gtttggcggc
      481 aaccccgtag cgtgcgccgc ctcgctggcg acgatcaagc tgctcgaaac gcgctaccgt
      541 gccaatgccg agcggcgcgg ccaccagctt cacgccggcc taaccgcgct ggctcatcgt
      601 ttttccatga ttcgcgaggt gcgggggctg ggactgatga tcggggcaga gatccaatcc
      661 gaagcgggag agcctgacgc caccctgcgt gaccaaatcg tcgagatcgc gttccggcgt
      721 ggtctgctct tgctgccgtg cgggccgagc acagtccggt tttgcccgcc attgtgcgtg
      781 aaccggcgac agatcgagat cggtctcgag ttgttctcaa gcacgttaga agccgctctt
      841 ggcgagatga agcgaggaga gcccgacggc gcggactcga tccccgagct cgaggagctc
      901 gcccaggaac ccgcgtgatg ccaatgggcg acccttgctc ggatcgggtc gtcgcaccgg
      961 aattacggag cttaggagac tagccatgac ggtgatcgcc gagtccgccg ccccggttgt
     1021 gtttgactgg aagcgctggc cgcggacgga attactggtc gacgagttga ttgagacggc
     1081 cctgagcggc aatgcgttcg caactgaact ggcgggccgg atgagccgcg agaccgggac
     1141 cagattcaag gactgggtcg atcaccttgt gatcgcggac gaagaaggtc tttcccaccg
     1201 actggaggag gctggcttcg agcggcaaag ggccgcgtac gccgtcggaa taccagtgtt
     1261 cgcacatccg ggcggaatct ttccccggat cgctctggcg agtcgccaaa agcggtccaa
     1321 cggggcgcca ggcggtgtcg taagtgaggt ggcgatcaaa gtggaaagtg tcgccgattt
     1381 ttcccgcgcg catgatctgg gtttggagat ggtcggctat tcgatgggcc cataccgagt
     1441 cggccgagtt cccggcgata agaccgcgct ggccgtggtc gagcggcggg gctacctggg
     1501 cttcgagccg tttcctggcg aactggcccg cgaggggcgg atgaagccgc acgcggcacg
     1561 cgatgccctg gcggcacgcg acctctggca ggcgcggcgc cgctcttttg atgacgatct
     1621 tgagggattc gacgccacag aggcgacact cgagcgcgtg atcgagctgg cgggctcgac
     1681 cgacctggcg tgtcatctgg tcttcgaggt cgagcgagat tactggcagt cgcgcaacaa
     1741 ggcggcgcgc gctcaaaaag cgcggcagga tcgactgggc ctggggtggg ccaatcatga
     1801 ccatcatacc ttccgctgtt cgcggcggtt cttttcccgc gtgatcggca tgtttagccg
     1861 cctcggcttt gcgctacgtg agcggttcca cgccggagag catgccggct ggggtgcaca
     1921 gatcgtcgag catccggtca ccggcatcgt catcttcgcc gatctcgact tggcccccga
     1981 ggaagccacg gccgactttg cccaccaatc attgccggat ttacctcggc ccgggacggt
     2041 cgggttgtgg gtcgccttgc acggcgagtc gatcttagaa gccggcatgc accaccttga
     2101 ggcccagttt gacttcgacg ccctgcgcga cggcctcaaa gaggatgcgg gtatcgaaac
     2161 gatgccgccg ttctccaatt tcccgttctt gaggcaggca ttcactgcgg gcgagcgctg
     2221 gccggtcgcc aagcatcggg tcgaccgggc actttcgcag ggttggatca ccgccgttca
     2281 acatgagaaa ttccttcgtg aaggtgcgat tggcagccac ctcgagaacc tcgaaaggcg
     2341 cgaaggctat aaaggtttca atcagcaggc ggtctccgcg atcattgccg ccaccgatcc
     2401 cagactgcat taaagaccag cgtggaaaca acttgactga ttagcgtttg cggtctaccg
     2461 attgcgtagg gtgggcactg cccaccaacg ggagcctggt gggcagtgcc caccctacaa
     2521 aatcgcgaag atcgtttaag ttaatttcgc gcggatcctg agacagccaa gccggcaaa
//
LOCUS       DATAQL010001475         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_4426196, whole genome shotgun sequence.
ACCESSION   DATAQL010001475 DATAQL010000000
VERSION     DATAQL010001475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4426196"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VHS97_25535"
     CDS             complement(<1..463)
                     /locus_tag="VHS97_25535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3451642.1"
                     /translation="MIYKRTLIPAAFVSIGFVSLAISLCSGADEPPPPRPRPGLTAKQ
                     TKEAVKIAQGAMVELRKKTEGASAPGADVREYVVGVELLATSVASSPSTTKPTPPAET
                     EPAETKAPAKEKENGKESETEKENRPSPGPRAIVTSYRYFDDITVFSTIDLA"
     gene            complement(778..>3267)
                     /locus_tag="VHS97_25540"
     CDS             complement(778..>3267)
                     /locus_tag="VHS97_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX3451643.1"
                     /translation="LPILALNPMKGGAAVIDFSDIFMTDFAQLGLGMLDRSRSTWTKV
                     KGFPNNVELEVEATFAGGGGRRWHMGAGDSVADHRGITVVIHYSLMKTPDFGYRPRKA
                     DDRVGHFLNATKDYGVTDTDTNFVRLINRWRLEKANPASKLSPPKKQIVWYVEDTVPI
                     EYRPYVEEGIREWNKAFEKIGFKDAIAVRWEESGRDDFDPEDTNYCTFRWVTAENNGL
                     IAMSCFRANPLTGEMIDGDVVFDAGFIRYMKQQYALLIGSMTALDGQPQTAPLAIGEL
                     VSPILASKLGYGQPNASALLGVDALDKSPNQMTAEIVPADQNAITWQLGRNLARGSRG
                     FCQSQFGLQRDFGLAAIAMAESYDDPKPADEKKPEEKKDEKDKDKEKKKPQPKDELPE
                     EFIGQAIKETVMHEVGHSLGLRHNFKASTMLPADQLHDTTITRAKGLVGSVMDYNPIN
                     IAPKGKKQGDYYTTTIGPYDYWAIEYAYKPIDGDEATELKKIASRAPEHDLAYGTDED
                     SFLNDDPYVNLWDLGSDPCQFGKDRIALATELMKDLDARVVKDGESWARTRRAFGMLL
                     EQWGDGATLASQYVGGQSVSRDHKADKDAHDPIAPVPGAKQRECLKFLADTILSDKSF
                     QFSPTLLRRLGTEHWSHWGNDHTFGAGVNISVLERILAIQKIVLGQCLSSSTLARLEN
                     QQLQADPGSDPLRVDELFRALTDGIWSDLDHLPATSDDKPGKFALTTIRRNLQREYLR
                     RLSGMVIGTTPNTYGDSLGFVVFLRGGNSSVPADARALARLHLREIANRIGKVLEAKN
                     LTIDDTCKAHLEECRHRISKVLEANLDVREP"
BASE COUNT          548 a          972 c         1013 g          734 t
ORIGIN      
        1 tcgccaggtc gatcgtggaa aagaccgtga tgtcgtcgaa atagcgatac gacgtcacga
       61 ttgcgcgcgg gccaggactc ggccggttct ctttttctgt ctcactttct ttcccgtttt
      121 ccttttcctt tgcgggcgct ttggtctcgg ccggttcggt ctcggcagga ggcgttggct
      181 tggttgtgga cggcgaactg gctacgctgg tggccagcag ctcgaccccg accacatact
      241 cacgcacgtc ggcgccgggg gcggacgctc cttccgtctt cttgcgcagc tcgaccatgg
      301 cgccctgggc gatcttgacc gcctctttgg tctgcttggc ggtcaatccg ggccgcggcc
      361 gcggtggtgg cggctcgtcg gcaccagagc acagagaaat cgccaggcta acaaagccaa
      421 tgctcacaaa ggcagcgggg atcaatgttc gtttgtaaat catctcggtt ccctcgcgac
      481 tcacggtcct taaggccccc tcgccggccc tgcgggtggg actgatgaac ggtaggtctt
      541 ccccgcccgg acgttcaacc ttttttgttg cgcctgctgg cgccccttga gaggttggtg
      601 gctgtggtcg aagccggctt caatccggcg agcccacagt ggtcgtcact gggggcgtcg
      661 ccctttctga cgacgatccc agccacccag tcttgcctgt atagcgcaac ccaatccgaa
      721 ttcgatcatc ggctccagat atcggcgagg gggccaccgt cgtgatgccc cccgcgctca
      781 cggctcgcgg acgtcgaggt tggcctcgag gactttgctg atccggtgcc ggcattcctc
      841 gaggtgagct ttgcaggtgt cgtcgattgt caggttcttt gcctccagga ctttaccgat
      901 ccggttggca atctcccgaa gatggagccg agctaatgcg cgggcgtcgg cgggtactga
      961 ggagttaccc cctcgcaaga agacgacaaa gccaagcgag tcgccgtagg tgttgggggt
     1021 ggtgccgatg accatgccgc tcaaccggcg caggtactcg cgctggaggt tgcgacggat
     1081 tgtcgtcaaa gcaaacttgc cgggcttgtc gtcgctcgtt gcgggcaaat gatccaggtc
     1141 ggaccagatc ccatcggtca gggctcggaa aagctcatcg acgcgcagcg ggtcggagcc
     1201 tggatcggcc tggagctgct ggttttcgag gcgtgccagc gtcgaggagc tcaagcactg
     1261 accgagcacg atcttctgaa tcgccaggat gcgttcgagc accgagatat taactccggc
     1321 tccgaacgtg tggtcgttgc cccagtgcga ccaatgctcg gttcccagcc gccggagcaa
     1381 cgtcggcgag aattggaacg atttgtcgct caggatggtg tcggcaagaa acttgaggca
     1441 ctcgcgctgt ttggctcccg gcacgggagc gatcgggtcg tgcgcgtcct tgtcggcctt
     1501 gtgatcgcgc gagaccgatt ggccccccac atactgggaa gccagggttg ccccgtcgcc
     1561 ccattgctcg agtaacatgc caaaggcacg ccgcgtccgc gcccacgatt cgccgtcttt
     1621 taccacacgg gcgtcgagat ctttcatcag ttccgttgcc aaggcgatcc ggtccttacc
     1681 aaactggcac gggtcggatc ccaggtccca gagattcacg taggggtcat cgttcaagaa
     1741 agagtcttcg tcggtcccgt aggcgaggtc gtgctcggga gcgcgtgagg cgatcttttt
     1801 gagctcggtt gcctcgtccc cgtcgatggg cttgtaggca tattcgatcg cccagtaatc
     1861 gtaaggaccg atcgtggtgg tgtagtaatc cccctgcttt ttccccttgg gggcgatgtt
     1921 gatcgggttg taatccatga cgctccccac caggcccttg gccctggtga tcgtggtgtc
     1981 gtggagctga tcggctggca gcatcgtgct ggccttgaag ttgtgccgca gtcccaacga
     2041 gtggccgact tcgtgcatca cggtttcctt gattgcctga ccgataaact cctcgggcag
     2101 ctcatccttg ggctgaggct ttttcttttc tttgtctttg tccttttcgt ctttcttttc
     2161 ctctggtttc ttttcgtccg ccggcttggg gtcgtcgtac gactcggcca tggcgatcgc
     2221 ggccagaccg aaatcgcgtt gcaggccaaa ctgagattgg caaaagcctc ggcttccccg
     2281 cgccagattc ctgccgagct gccaggtgat cgcattttga tcggcgggta cgatctcggc
     2341 tgtcatctgg ttggggctct tgtccagcgc atcgacgccc agcaaggcgc tggcattggg
     2401 ctggccataa cccaatttcg acgccaggat tgggctgacc agctcgccaa tggccagcgg
     2461 cgccgtctgc ggctgaccgt ccagggccgt catgctgccg atcaaaagcg cgtactgttg
     2521 cttcatgtag cgtatgaagc cggcgtcgaa cacaacgtcg ccatcgatca tctcaccggt
     2581 cagcgggttg gctcgaaaac acgacatggc aatcaggcca ttattctcgg ccgtgaccca
     2641 gcggaacgtg cagtagttgg tgtcttcagg gtcgaagtca tcgcggccgg actcttccca
     2701 gcggacggcg atggcgtctt tgaagccaat cttctcgaac gccttgttcc attcccggat
     2761 cccctcctca acgtacgggc ggtactcgat ggggacggtg tcttccacgt accagacaat
     2821 ctgcttcttc ggcggcgaga gcttggaggc aggattggcc ttttcgagcc gccagcggtt
     2881 gatcagccgg acaaagttcg tatcggtgtc ggtgacgcca tagtctttgg tggcattcaa
     2941 gaagtggccg acgcgatcgt cggctttgcg gggacgatag ccgaaatccg gggtcttcat
     3001 gaggctgtaa tgaatgacaa cggtgatgcc ccgatggtcg gcaacgctgt cgccggcgcc
     3061 catgtgccaa cggcggcctc cgccgccagc gaaggtggct tcgacctcga gctccacgtt
     3121 attgggaaaa cccttgacct tggtccaggt gctgcggctg cgatcaagca tgccgaggcc
     3181 gagctgggca aagtcggtca tgaagatgtc ggaaaagtcg atcacggccg cgcccccttt
     3241 catcgggttg agcgcgagga tcggcag
//
LOCUS       DATAQL010001476         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_915814, whole genome shotgun sequence.
ACCESSION   DATAQL010001476 DATAQL010000000
VERSION     DATAQL010001476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915814"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VHS97_25545"
     CDS             complement(<1..821)
                     /locus_tag="VHS97_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3451644.1"
                     /translation="MSLANTSHEYDVVVLGGGAAGLVAAARAAECGARVLLCEKNRRP
                     GVKILMSGGTRCNITNARGLRRLEVVSGSIDPAYDRGQSRGIRSIQQAFGDKGNFLAP
                     SLRRFDVDATVRMFEDAGVATKIEGNGKIFPVSDRATDVLEALSRRLERSGAVFRALS
                     PALGVERLDTEPGQGSAFTVKLADSSVTAQRVIVAIGGASYPGCGTTGDGYAIARRFG
                     HTIVEPRPALVPLRVKADWVPGLKGLSLPDVVASVYSARNELLAQRREAILFAHFG"
     gene            complement(945..2261)
                     /locus_tag="VHS97_25550"
     CDS             complement(945..2261)
                     /locus_tag="VHS97_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3451645.1"
                     /translation="MTLTVASPLKQVSTSNGRSAFYPRSIENFDEAGLNAAIVESLVL
                     KFLLNVGMAPGRRVAAELGLPFGPFPDFLRGLKNQQILAYANSASANDYIYSLTDTGR
                     ARAKMYVEECAYVGPAPVPFDDYLKSVALQTITNEHPKEDDLRRAFADLLIAEETFRL
                     LGPAINSGRGLFLYGYPGNGKTSIAERITRCFGTNIWIPKVIQAEGQIIKLFDMANHE
                     PVETEQSPGLLDESDHDHRWIQIKRPTIVVGGELQMEDLEIHSDPVTKISEAPLQLKS
                     NLGTFLIDDFGRQRMQPVELLNRWIVPLEKRYDFLSLAGGKKIRVPFDQLIIFSTNLE
                     PRQLVDEAFLRRIPYKIQAADPTEDMFRKMIAMFAPKLGFKEVDGTAIDYLVDRHYQR
                     ASRPFRCCHARDLLLQVRNYCMYNDVPLELKPEYFDMAVTNYFTMT"
     gene            complement(2386..>2631)
                     /locus_tag="VHS97_25555"
     CDS             complement(2386..>2631)
                     /locus_tag="VHS97_25555"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX3451646.1"
                     /translation="IYHFDAYRLENPQAFEDLGVADYWGAGGVCLVEWADRVRGLLPD
                     DCWLISIQQSGPVARRVRIEIPESAAVILERLAERLT"
BASE COUNT          538 a          792 c          808 g          493 t
ORIGIN      
        1 ccgaagtggg caaaaagaat cgcctccctg cgctgcgcta gcagctcatt cctggcggaa
       61 tagaccgagg cgaccacgtc cggcagggag agccccttga ggccgggaac ccagtcggcc
      121 ttgaccctga gcggcacgag cgccgggcga ggttcgacga tcgtgtggcc aaatcgacgc
      181 gcgatcgcgt agccgtcgcc cgtggtgccg cacccagggt acgacgcacc gccgatcgca
      241 acgatcaccc gctgcgcggt cacggacgaa tccgccagct tgaccgtgaa agccgagcct
      301 tggccaggct cggtgtccag tcgctccacg ccgagggcag ggctcaaagc gcggaacacc
      361 gctccagatc gttcaagccg ccgcgagagc gcttcgagaa cgtcggtggc gcggtctgag
      421 accgggaaga tcttaccgtt tccttcgatc ttggtcgcga cgccagcatc ttcgaacatc
      481 cgcacggtcg cgtcgacgtc gaaccggcgc aaggagggag cgaggaagtt tcccttatcg
      541 ccgaaagcct gctggatgga ccggatgccg cgcgactggc cccggtcgta ggccgggtcg
      601 atcgagcccg acaccacttc gagccgccgc aggccgcggg cattggtgat attgcaccgg
      661 gtgccaccgg acatcaggat cttgactccg ggacgccgat tcttctcgca cagtagcacg
      721 cgcgcgccgc actccgcggc ccgcgccgcg gcgaccagcc ccgccgcccc accgcccagg
      781 acgaccacat catattcgtg ggatgtattc gcgagactca cggaatcccg tcaaaaaggg
      841 gtcaggaccc tttttcgccc agcctgcgtg atccggctct ccgacggatt gatgatagca
      901 caacgggacg tcgggatacg tcgcgcattc gatccgcgga ccgttcacgt catcgtgaaa
      961 tagttcgtca cggccatgtc gaagtattcg ggcttgagct caagtggcac atcgttgtac
     1021 atgcagtaat tacggacctg taaaagaaga tcgcgggcgt ggcaacagcg aaacggacga
     1081 ctggctcgct ggtaatggcg atcgacaagg tagtcgatcg ccgtcccatc cacttccttg
     1141 aacccgagct tgggagcgaa catggcgatc attttccgga acatgtcctc ggtagggtcg
     1201 gcggcctgga tcttgtaggg tatccggcgg agaaacgctt catcgacgag ctggcgcggc
     1261 tcgagatttg tcgaaaatat aatgagttga tcaaacggaa cccggatctt cttgccgccg
     1321 gcgaggctca agaagtcgta acgcttttcg aggggcacga tccagcggtt caagagctcg
     1381 accggctgca tccgctggcg gccgaaatcg tcgatcaaga aggttcccag attgctcttc
     1441 aattgcagtg gggcctcgct gatcttggtc acgggatcag agtggatttc caggtcctcc
     1501 atttgcagct cgcccccgac aacgatcgtc ggacgcttga tctgaatcca gcggtggtcg
     1561 tggtcggact cgtcaaggag gccaggggat tgctcggtct caaccggttc gtgattcgcc
     1621 atgtcgaaca gtttgatgat ctgcccttcc gcctggatca ctttgggaat ccagatgttc
     1681 gtgccgaagc agcgggtaat tcgctcggcg atactggtct tgccgttgcc gggataacca
     1741 taaagaaaga ggccacggcc cgagttgatc gccgggccca gcaggcggaa tgtctcctcg
     1801 gcgatcaaga ggtcggcaaa agcccgcctc aagtcgtctt ccttgggatg ttcgttcgtg
     1861 attgtctgga gggccaccga cttcaaataa tcgtcgaacg gaaccggtgc agggccgacg
     1921 taggcacact cttccacata catcttggcg cgcgcccggc cggtgtcggt aagcgagtag
     1981 atataatcat ttgcagatgc agaattcgca taggcgagaa tctgctggtt cttgagcccg
     2041 cgaagaaaat cgggaaatgg tccaaaaggc aagccgagct cggccgcgac ccgccgaccg
     2101 ggtgccatgc cgacattgag caggaacttg aggaccaggc tttctactat cgccgcgttg
     2161 agcccggctt cgtcgaagtt ctcgatggat cgcgggtaaa aagcgctcct gccattagag
     2221 gtcgagacct gtttcagcgg tgaagcgact gtaagggtca cgtcaagagt ctcccttcgg
     2281 ttgagcgcgg caatgatctg atccacggcc tggcccggcg catggtatcc cgcacaaccc
     2341 taggtcatgc acacaaaggc gtcaaaccgc ccggcaccaa ctgtttcacg tgagccgttc
     2401 ggccagtcgt tcgaggatga cggcggccga ctcgggaatc tcgatccgga ctctgcgcgc
     2461 cacaggcccg ctctgctgga ttgaaatcaa ccagcagtcg tcaggtaaga ggccgcggac
     2521 gcgatcggcc cattcgacca gacagacgcc gcccgcgccc cagtagtcgg cgacaccgag
     2581 gtcttcaaag gcctgcggat tttcgagacg gtacgcgtcg aagtggtaga t
//
LOCUS       DATAQL010001477         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_3511245, whole genome shotgun sequence.
ACCESSION   DATAQL010001477 DATAQL010000000
VERSION     DATAQL010001477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511245"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..993)
                     /locus_tag="VHS97_25560"
     CDS             complement(154..993)
                     /locus_tag="VHS97_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3451647.1"
                     /translation="MNSNSAVERESPPKILELARCLSCGNPLAGEPICLACGRAYPTR
                     DGITEAIGPLAGRNQIVADFYDGPGWVRFRPWEQAFLIFQGGVRRARMEILHHVFGVD
                     TATARVLEVGIGSGENLTFLRRGWDVFGIDIARTQLEACLRRHPAMAGHLAWAEAERL
                     PFADAAFDASWSVGGFNYFSDHERALREMRRVTKPGSPVVVADEVPGLHRAGLGHLLG
                     IPSLDAWWLRKLGLDREFVRVVLEFNVDLTGLANRVWPHHTRHRIWHGMGYCLVDKSG
                     LSE"
     gene            complement(1013..1795)
                     /locus_tag="VHS97_25565"
     CDS             complement(1013..1795)
                     /locus_tag="VHS97_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3451648.1"
                     /translation="MSARPIVLLTGAAHGIGHATAVALARTGTPLGLIDRDAASLGEL
                     VKELKDQGATVAHAAVDVTDRHALIRAVADINAALGAIDVLVACAGIGSLTQVPQLET
                     ITLRQTLDVNLVGVAQSIEAVLPGMITRGRGHIVGVASVAGYRGFPWMISYSASKAAL
                     IAYLEAVRPGLRRRGVTVTTVCPGFVRTQMSIDIPYRQPVKMIEPEVAARYLVRAIDR
                     RPRNCVFPWDMRIGLAILKYMPDWFFDRLMYWFGPRALKIEF"
     gene            complement(1792..2649)
                     /locus_tag="VHS97_25570"
     CDS             complement(1792..2649)
                     /locus_tag="VHS97_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3451649.1"
                     /translation="MPKLMANGLNFHYQQAGSGPDVILIHGVTGDLSIWFLCEAMGVL
                     ARSFRVTAFDLRGHGYSDVPRDGYTSADQAGDVLAIMDGLGIERAALVGHSFGAVIAT
                     HAAVLYPGRIDGLVLSDPSFPALRHLENLSRWGHWQNFREEAAQAGVTLSDEHWYDLG
                     RFFDQVLHLDGERLLRFRQAVGLPGFNRLLRLAHTTCGDDAKAIAGLTESMIQSVTQP
                     VLAIFGEHSPFLATALYLGQHLAHCVNRLVPGAKHRAPEENGPMFVELVNEYLTSQES
                     TAQLARTTR"
BASE COUNT          598 a          902 c          863 g          501 t
ORIGIN      
        1 tgcgagcact gcatgaccga cgttgccgga acgtattgca acgcgccctg gcaatcgagg
       61 caccgccaga tgtcccagtc agattgccag tgtgaggttg tgacctgccg ggggctgatg
      121 gtcgacatgg agagactccc aaattggatg agattattcc gacaagccgg acttgtctac
      181 aagacaataa cccatcccgt gccagatgcg gtgccgtgta tggtgcggcc acacacggtt
      241 ggcaagaccg gtgagatcga cattgaattc gaggaccacc ctgacgaact cacggtcgag
      301 cccgagcttg cgcagccacc aggcatcgag tgacggaatg cccaacaaat ggcccaggcc
      361 ggcgcgatga agcccgggga cctcatcggc gactaccacg gggctgcccg gtttggtcac
      421 gcggcgcatc tcgcgcagtg cccgctcgtg gtcgctgaaa tagttgaacc cgccgaccga
      481 ccaggaagcg tcaaacgcgg catcggcgaa cggaaggcgc tcggcctcgg cccaggccaa
      541 gtgtcctgcc atagcgggat gccggcgcag gcacgcttca agctgggtcc gcgcgatgtc
      601 gattccaaaa acgtcccatc cacggcgcag aaaggtcaga ttttctccgc taccgatccc
      661 gacctcgagc acacgagcgg tggccgtatc aactccgaag acatggtgca agatctccat
      721 acgggcccgg cgaactcccc cttggaaaat caaaaacgcc tgttcccagg gccggaacct
      781 gacccagccg ggaccgtcat aaaaatcggc gacaatttgg ttgcgcccgg ccagcggtcc
      841 gatcgcctcg gtgatcccgt cccgcgtcgg atatgcgcgt ccgcacgcca ggcagattgg
      901 ttcgcccgcg agcgggttgc cacacgatag gcagcgcgcc agctcaagaa tcttgggcgg
      961 tgattcacgc tcgacggcgc tattcgagtt catcgtgctc tctctggcca ttctaaaact
     1021 cgatcttgag cgctcgcggt ccaaaccagt acatgagccg atcaaaaaac cagtctggca
     1081 tgtacttgag gatcgcgagg ccgatcctca tgtcccaggg aaagacgcaa ttgcgcggcc
     1141 ggcgatcgat ggcacggacg aggtatcgtg ccgccacctc gggctcgatc attttcaccg
     1201 gctgccgata cggtatatcg atgctcattt gtgtgcgcac gaacccaggg cataccgtgg
     1261 tcaccgtcac cccacgccgg cgcagaccgg gccgcactgc ctcaagataa gcgatcaggg
     1321 ccgccttgga ggcggaatag gaaatcatcc agggaaaacc acgatagccg gccacgctgg
     1381 ccacaccaac aatgtgaccg cgcccgcgtg tgatcatccc gggtaagacg gcttcgattg
     1441 attgagccac acccactaaa ttgacgtcca gcgtctgccg cagcgtgatc gtttcgagct
     1501 gcgggacctg ggtcagcgaa ccgatcccgg cgcacgcgac caggacgtcg attgccccaa
     1561 gcgcggcgtt gatatcggct accgcccgga tcaaggcatg ccggtccgtg acgtcgaccg
     1621 ccgcatgggc gacagtcgca ccctggtcct tcagctcttt gacaagctcc ccgagcgacg
     1681 ccgcgtcgcg atcgatcagg ccgagcggag tgccggtccg ggccagcgcc acggccgtcg
     1741 catggccaat tccgtgagcc gcccccgtca atagcacgat cggtctggca ctcatcgtgt
     1801 cgttctcgcg agttgagccg tggattcttg agacgtcaga tattcattca caagctcgac
     1861 gaacatcggt ccgttttctt caggagcgcg gtgtttggcc ccgggcacca gacggttgac
     1921 gcaatgagcg aggtgctggc ccagataaag ggcggtggcc aggaatgggg agtgctcacc
     1981 gaagatggcg agtaccggtt gagtcacgct ttggatcatg gattcggtaa ggcccgcgat
     2041 cgccttggcg tcgtcgccgc atgtggtatg tgccagtcgc aacagacgat tgaagcccgg
     2101 caagccgacg gcctggcgaa accgcaagag ccgctcgccg tccaggtgga gcacttgatc
     2161 gaaaaaccgg cccaggtcat accaatgctc atcggacagc gtgacaccgg cctgagccgc
     2221 ttcctcgcgg aagttttgcc agtgacccca tcggcttaag ttttccaggt gacggagagc
     2281 cggaaatgac ggatccgaca ggaccaaacc gtcgatcctg ccgggataaa gcaccgccgc
     2341 gtgcgtggcg atcaccgcgc cgaagctgtg cccgaccagc gccgcacgct cgatgcccag
     2401 gccgtccatg atcgccagga cgtcgcctgc ctggtcggcc gaggtgtatc catctcgagg
     2461 aacatcgctg tagccgtgtc ctctcaggtc gaaggccgtg accctgaacg agcgcgcaag
     2521 cacccccatg gcctcgcaca gaaaccagat ggacaagtcg ccggtgacac cgtgaatcaa
     2581 gatcacgtct ggcccggagc cagcctgctg gtagtgaaag ttcaatccgt tcgccatgag
     2641 tttcggcatg agggaagtct cgcctgatat tatgaatcgt ttgcgcttgc aagattctcg
     2701 gccagaaact cgaccagctc gccgatcgcg aggtcgcgct gggtccttcg accaatctcc
     2761 gccatcagct cgttgaacgg cagagggcgg ccgtaatgct actgaagtct ttcgcccagg
     2821 acgacggcat cgatcgacgc caagccgagg tcggcgaaga agcg
//
LOCUS       DATAQL010001478         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Isosphaeraceae bacterium isolate SMAG_U5466
            k141_1678699, whole genome shotgun sequence.
ACCESSION   DATAQL010001478 DATAQL010000000
VERSION     DATAQL010001478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956291
            Sequence Read Archive: SRR11836028
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Isosphaeraceae bacterium (soil metagenome)
  ORGANISM  Isosphaeraceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Isosphaerales;
            Isosphaeraceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:00:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,116
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 24 of 37
            Pseudo Genes (internal stop)      :: 9 of 37
            Pseudo Genes (multiple problems)  :: 4 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Isosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678699"
                     /isolate="SMAG_U5466"
                     /isolation_source="soil"
                     /db_xref="taxon:2691532"
                     /environmental_sample
                     /country="Australia: New South Wales"
                     /lat_lon="34.5 S 150 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VHS97_25575"
     CDS             <1..686
                     /locus_tag="VHS97_25575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX3451650.1"
                     /translation="QPEAPAHPVTLATFYIDKHEVTLRQYALFLKESGHKDSERQRAL
                     AREASGGCDSEDCPVVMVSAYEARDYAKWAGKVLPTEAQWEMAARGTDGRPFPWGMTA
                     PAWDKKREPRQVDPIMSFPADVSPYGVYDMAGNAMEWTKDWFDPNYYKHFRKSPAIDP
                     TGPSRTRTVQLAVKGGSKTWIITKRDGIKPESRLATLGFRCALQADPPQAAPPADAAG
                     GGVPSMVPF"
     gene            complement(870..>2401)
                     /locus_tag="VHS97_25580"
     CDS             complement(870..>2401)
                     /locus_tag="VHS97_25580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3451651.1"
                     /translation="TLYKLTPDGKVRWSYRNPAYQGAPSQAEARPKAEQPSTVRLQPS
                     DEGSILGSALVSGDTVFFSDLGGWIYALDRATGKERWKLSTRGKEFPGAHPMYLSFAS
                     PILAQGKLIVAGGTSEQARAAMPGYKGCTGRGFVAALEPQTGRVTWKYDVGPEPQPLD
                     PPITIEDSSGKHTFYYGPATSSVWSTPSFDAESGTVFFGTDVNTAPRRPTADNPRLHT
                     RESCAVIALDVRDGAEKWVTQINPGDVWTNAMRSYDPKDGRYKDQSIGDTPKIYTIPV
                     EGKATKVVGVGCKNGGFYVLGAMDGRVLDHTPIYTGPPTYPLSPEPDRRMLALPSAIG
                     GLQTGCATDGETIFTNGIDALWLGSLKPPLPSGGYVSGGRVVALSLDTRTERWRHERP
                     KVASLGGPPPKRVFTDVGDPVASGIAVANGVVYFTAVASGKLTALDAATGEVLKEIGL
                     GPVWSGPSVSRGRVYVGTGNTLLTPSAYQAFLPKKYTGVLYSFGLPGEDEVSRLGAGK
                     E"
BASE COUNT          401 a          802 c          802 g          396 t
ORIGIN      
        1 agcagcccga ggcccccgcc cacccggtca cgctggccac cttttatatc gacaagcatg
       61 aagtgaccct gcggcagtac gccctcttcc tcaaggagtc ggggcacaag gacagcgaac
      121 gacaacgcgc cctggcccgc gaggcgtccg gcggctgcga ttcggaggat tgccccgtcg
      181 tcatggtcag tgcctacgag gcccgcgact atgccaaatg ggccgggaag gtgctcccga
      241 ccgaggcgca gtgggagatg gccgcgcgcg ggaccgacgg ccgaccgttc ccctggggga
      301 tgaccgcgcc cgcctgggac aagaagcgcg agccgaggca ggtcgacccg atcatgtcgt
      361 tccccgccga cgtctcgcct tacggcgtct atgacatggc cggcaacgcg atggagtgga
      421 cgaaggactg gttcgacccg aattactaca agcacttccg gaaatcgccc gccatcgacc
      481 ccaccggccc gagccggacc cggaccgtgc aactggcggt caaggggggc tcgaagacct
      541 ggatcatcac gaagcgcgac gggatcaagc ccgaatcccg gctggccacc ctcggcttcc
      601 gctgcgcctt gcaggccgat cctccgcaag ccgccccccc cgccgacgcg gccgggggcg
      661 gagtcccgag catggttccc ttctgagcga aaagggggcc agcctccggc tcagggacta
      721 tatcgttcaa tgagagcggt tcacgtacgg tttgcagtcg atgggtaggg ccggccattg
      781 ccggccatct tgagaaagct cggccggcaa tggtcggccc gagtggcatc gcgcccgtgc
      841 cgttcgaccc ctacgtggaa cattgccctc tattcctttc cagcccccag acgactgact
      901 tcgtcctcgc ccggcaaccc gaaggagtac aggacgcctg tgtatttctt cggcaagaag
      961 gcctggtagg ccgacggtgt gagcagcgtg ttgcccgtgc cgacgtagac ccggccgcgg
     1021 gaaaccgaag ggcccgacca caccgggccg aggccgatct ccttgagcac ttcgcccgtg
     1081 gctgcatcca gggcggtcag ctttccactg gcaaccgctg tgaagtaaac gaccccattg
     1141 gcgacggcga tccccgaggc gaccgggtcg ccgacgtcgg tgaagacgcg cttcggcggc
     1201 gggccgccga gcgaggcgac tttgggccgc tcatgccgcc accgctccgt gcgcgtatcg
     1261 aggctcaggg ccacgacacg accgcccgac acataaccgc cgctgggcag cggaggcttc
     1321 agcgacccga gccagagagc gtcgatgcca ttggtgaaga tcgtctctcc gtcggtcgcg
     1381 cagccggtct gcaagccccc gatggcgctc ggcaaggcga gcatccgccg gtccggctcc
     1441 ggcgaaagcg ggtacgttgg cgggccggta tagatggggg tgtggtccag gacgcgcccg
     1501 tccatggccc ccaggacgta gaagcccccg ttcttgcagc ccacgccgac caccttggtg
     1561 gccttacctt ccacggggat cgtgtagatc ttgggcgtgt cgccgatcga ctggtccttg
     1621 taccgcccat ccttcgggtc gtaggaccgc atggcgttgg tccagacgtc gccgggattg
     1681 atctgagtca cccacttctc ggcgccgtcg cggacgtcga gggcgatgac cgcgcacgat
     1741 tcgcgcgtgt gcagtcgggg gttgtcggcg gtcggccgcc gcggagcggt gttgacgtcc
     1801 gtgccaaaaa agaccgtgcc agactccgcg tcgaacgacg gggtgctcca gacgctgctc
     1861 gtggccgggc cgtaataaaa ggtgtgcttg cccgagctat cctcgatcgt gatcggcggg
     1921 tcaagaggct ggggctcggg gccgacgtca tacttccacg taacccgccc ggtctgtggc
     1981 tccagggcgg cgacgaatcc ccggccggta cagcccttat agccgggcat ggcggccctg
     2041 gcctgctcgg acgtgccgcc ggcgacgatg agtttgcctt gggccaggat cggcgaagcg
     2101 aaggagaggt acatcggatg ggcgccgggg aattctttgc cccgggtgct cagcttccaa
     2161 cgctccttcc cggtcgcccg gtcaagcgcg tagatccagc ctcccagatc gctgaagaag
     2221 acggtgtcac cgctgacgag tgccgatccg aggatacttc cctcgtcgga cggttggagc
     2281 cgcacggtgc taggctgctc tgccttcggc cgcgcttcag cttgagaggg cgcgccctgg
     2341 taagccgggt tacggtacga ccatcggacc ttgccgtcgg gtgtaagctt gtataacgtc
     2401 g
//
